bluuebunny/crossref_metadata_2025 huggingface dataset:
Influence of Pairing Startling Acoustic Stimuli with Postural Responses Induced by Light Touch Displacement
Authors: Misiaszek John E. , Chodan Sydney D. C. , McMahon Arden J. , Fenrich Keith K.
Published: January 2020

The first exposure to an unexpected, rapid displacement of a light touch reference induces a balance reaction in naïve participants, whereas an arm-tracking behaviour emerges with subsequent exposures. The sudden behaviour change suggests the first trial balance reaction arises from the startling nature of the unexpected stimulus. We investigated how touch-induced balance reactions interact with startling acoustic stimuli. Responses to light touch displacements were tested in 48 participants across six distinct combinations of touch displacement (DISPLACEMENT), acoustic startle (STARTLE), or combined (COMBINED) stimuli. The effect of COMBINED depended, in part, on the history of the preceding stimuli. Participants who received 10 DISPLACEMENT initially, produced facilitated arm-tracking responses with subsequent COMBINED. Participants who received 10 COMBINED initially, produced facilitated balance reactions, with arm-tracking failing to emerge until the acoustic stimuli were discontinued. Participants who received five DISPLACEMENT, after initially habituating to 10 STARTLE, demonstrated re-emergence of the balance reaction with the subsequent COMBINED. Responses evoked by light touch displacements are influenced by the startling nature of the stimulus, suggesting that the selection of a balance reaction to a threatening stimulus is labile and dependent, in part, on the context and sensory state at the time of the disturbance.
Acute Effects of Midsole Bending Stiffness on Lower Extremity Biomechanics during Layup Jumps
Authors: Zhu Zhiqiang , Fu Weijie , Shao En , Li Lu , Song Linjie , Wang Wei , Liu Yu
Published: January 2020

Purpose: This study aims to investigate the acute effects of shoe midsole stiffness on the joint biomechanics of the lower extremities during specific basketball movements. Methods: Thirty participants wearing stiff midsole shoes (SS) and control shoes (CS) performed layup jumps (LJs) while the kinematics and ground reaction forces were simultaneously collected via the Vicon motion capture system and Kistler force plates. Furthermore, the joint angles, range of motion (ROM), joint power, joint energy, and jump height were calculated. Results: No significant differences were observed between SS and CS conditions for both jump height and the metatarsophalangeal (MTP) joint biomechanics except that the minimum angular velocity of the MTP joint was significantly lower in SS the condition. However, the ROM in the ankle joint was significantly greater in the SS condition than in the CS condition (p < 0.05). Additionally, the maximum plantarflexion power, energy absorption (EA), and energy generation (EG) in the ankle joint were significantly greater in the SS condition than in the CS condition (p < 0.05). Compared with the CS condition, jump height in the SS condition did not increase. Conclusion: During a single LJ, the longitudinal midsole stiffness did not influence the jump height and MTP joint biomechanical patterns but significantly increased the maximum power, EA, and EG during the push-off phase of the ankle joint. These preliminary results indicate that wearing SS could change the ankle joint mechanical patterns by modulating the lower extremity kinetic chain, and may enhance muscle strength in the ankle.
Virtual Bone Augmentation in Atrophic Mandible to Assess Optimal Implant-Prosthetic Rehabilitation—A Finite Element Study
Authors: Butnaru-Moldoveanu Sînziana Anca , Munteanu Florin , Forna Norina Consuela
Published: January 2020

The scope of our study was to analyze the impact of implant prosthetic rehabilitation, in bilateral terminal partial edentulism with mandibular bone atrophy, and potential benefits of mandibular bone augmentation through finite element analysis. A 3D mandible model was made using patient-derived cone-beam computed tomography (CBCT) images, presenting a bilateral terminal edentation and mandibular atrophy. A virtual simulation of bone augmentation was then made. Implant-supported restorations were modeled for each edentulous area. Forces corresponding to the pterygoid and the masseter muscles, as well as mastication conditions for each quadrant, were applied. The resorbed mandible presented high values of strain and stress. A considerable variation between strain values among the two implant sites in each quadrant was found. In the augmented model, values of strain and stress showed a uniformization in both quadrants. Virtually increasing bone mass in the resorbed areas of the mandible showed that enabling larger implants drastically reduces strain and stress values in the implant sites. Also, although ridge height difference between the two quadrants was kept even after bone augmentation, there is a uniformization of the strain values between the two implant sites in each of the augmented mandible quadrants.
Enhancing the Desalination Performance of Capacitive Deionization Using a Layered Double Hydroxide Coated Activated Carbon Electrode
Authors: Lee Jaehan , Kim Seoni , Kim Nayeong , Kim Choonsoo , Yoon Jeyong
Published: January 2020

Capacitive deionization (CDI) is a promising desalination technology because of its simple, high energy efficient, and eco-friendly process. Among several factors that can affect the desalination capacitance of CDI, wettability of the electrode is considered one of the important parameters. However, various carbon materials commonly have a hydrophobic behavior that disturbs the ion transfer between the bulk solution and the surface of the electrode. In this study, we fabricated a layered double hydroxide (LDH) coated activated carbon electrode using an in-situ growth method to enhance the wettability of the surface of the carbon electrode. The well-oriented and porous LDH layer resulted in a better wettability of the activated carbon electrode, attributing to an enhanced capacitance compared with that of the uncoated activated carbon electrode. Furthermore, from the desalination tests of the CDI system, the LDH coated carbon electrode showed a higher salt adsorption capacity (13.9 mg/g) than the uncoated carbon electrode (11.7 mg/g). Thus, this enhanced desalination performance suggests that the improvement in the wettability of the carbon electrode by the LDH coating provides facile ion transfer between the electrode and electrolyte.
Quantitative Analysis of Melanosis Coli Colonic Mucosa Using Textural Patterns
Authors: Lo Chung-Ming , Chen Chun-Chang , Yeh Yu-Hsuan , Chang Chun-Chao , Yeh Hsing-Jung
Published: January 2020

Melanosis coli (MC) is a disease related to long-term use of anthranoid laxative agents. Patients with clinical constipation or obesity are more likely to use these drugs for long periods. Moreover, patients with MC are more likely to develop polyps, particularly adenomatous polyps. Adenomatous polyps can transform to colorectal cancer. Recognizing multiple polyps from MC is challenging due to their heterogeneity. Therefore, this study proposed a quantitative assessment of MC colonic mucosa with texture patterns. In total, the MC colonoscopy images of 1092 person-times were included in this study. At the beginning, the correlations among carcinoembryonic antigens, polyp texture, and pathology were analyzed. Then, 181 patients with MC were extracted for further analysis while patients having unclear images were excluded. By gray-level co-occurrence matrix, texture patterns in the colorectal images were extracted. Pearson correlation analysis indicated five texture features were significantly correlated with pathological results (p < 0.001). This result should be used in the future to design an instant help software to help the physician. The information of colonoscopy and image analystic data can provide clinicians with suggestions for assessing patients with MC.
Modelling a Segmented Skutterudite-Based Thermoelectric Generator to Achieve Maximum Conversion Efficiency
Authors: Yusuf Aminu , Ballikaya Sedat
Published: January 2020

Thermoelectric generator (TEG) modules generally have a low conversion efficiency. Among the reasons for the lower conversion efficiency is thermoelectric (TE) material mismatch. Hence, it is imperative to carefully select the TE material and optimize the design before any mass-scale production of the modules. Here, with the help of Comsol-Multiphysics (5.3) software, TE materials were carefully selected and the design was optimized to achieve a higher conversion efficiency. An initial module simulation (32 couples) of unsegmented skutterudite Ba0.1Yb0.2Fe0.1Co3.9Sb12 (n-type) and Ce0.5Yb0.5Fe3.25Co0.75Sb12 (p-type) TE materials was carried out. At the temperature gradient T∆ = 500 K, a maximum simulated conversion efficiency of 9.2% and a calculated efficiency of 10% were obtained. In optimization via segmentation, the selection of TE materials, considering compatibility factor (s) and ZT, was carefully done. On the cold side, Bi2Te3 (n-type) and Sb2Te3 (p-type) TE materials were added as part of the segmentation, and at the same temperature gradient, an open circuit voltage of 6.2 V matched a load output power of 45 W, and a maximum simulated conversion efficiency of 15.7% and a calculated efficiency of 17.2% were achieved. A significant increase in the output characteristics of the module shows that the segmentation is effective. The TEG shows promising output characteristics.
Data-Driven Design Solution of a Mismatch Problem between the Specifications of the Multi-Function Console in a Jangbogo Class Submarine and the Anthropometric Dimensions of South Koreans Users
Authors: Lee Jihwan , Cho Namwoo , Yun Myung Hwan , Lee Yushin
Published: January 2020

The naval multi-function console provides various types of information to the operator. It is equipment that is key for submarine navigation, and fatal human errors can occur due to the mismatch between the console specifications and the operator’s body size. This study proposes a method for deriving console specifications suitable for the body size of Korean users. The seat height, seat width, seat depth, upper edge of backrest, and worktable height were selected as the target design variables. Using six anthropometric dimensions, a mismatch equation for each target design variable was developed. Anthropometric measures of 2027 Korean males were obtained, and the optimal specifications of the console were derived via an algorithmic approach. As a result, the match rate, considering all the target design variables, was improved from 2.57% to 76.96%. In previous studies and standards, the optimal console specifications were suggested based on the anthropometric data of a specific percentile of users, and it was impossible to quantitatively confirm the suitability of the console design for the target users. However, the method used in this study calculated the match rate using the mismatch equation devised for comfortable use of the console and a large amount of anthropometric data that represented the user population, and therefore the improvement effect of the recommended specification can be directly identified when compared to the current specifications. Moreover, the methodology and results of this study could be used for deciding the specifications of multi-function consoles in several fields, including nuclear power plants or disaster situation rooms.
Interface Crack Approaching a Three-Material Joint
Authors: Djoković Jelena M. , Nikolić Ružica R. , Ulewicz Robert , Hadzima Branislav
Published: January 2020

The problem of an interface crack that approaches a three-material joint with two interfaces is analyzed in this paper. Two possible cases are considered: the crack that lies at the interface between materials A and B, approaching the joint of materials A, B, and C, deflects into the interface between materials A and C or into the interface between materials B and C. Analysis is performed within restrictions imposed by the linear elastic fracture mechanics (LEFM), linear elastic behavior of materials, and the small plastic zone around the crack tip, based on the crack deflection criterion proposed by He and Hutchinson. That criterion is applied in this paper to a joint of the three homogeneous isotropic materials. The energy release rates for the crack deflection into one interface or the other are compared to each other, and, based on this comparison, a conclusion is drawn as to which of the two interfaces the crack would deflect. If the value of the ratio of the energy release rates GBC/GAC is greater than the ratio of the corresponding fracture toughnesses of the two interfaces, the crack will deflect into the BC interface. If this ratio is smaller than the ratio of the corresponding fracture toughnesses, the crack will deflect into the AC interface. Knowing the ratio of energy release rates for deflection into one interface or the other can be used for designing the interface, namely for prediction of the direction of further crack propagation.
New Frontiers in Stem Cell Research and Translational Approaches
Authors: Alessio Nicola , Siniscalco Dario , Peluso Gianfranco , Galderisi Umberto
Published: January 2020

Stem cell biology represents a challenging research area with a huge potential translational approach. This review focuses on the most recent findings on stem cell basics and clinics in several fields of research, as final outcome of the 10th conference held by Stem Cell Research Italy (SCR Italy) in Naples, Italy in June 2019. Current state-of-the-art and novel findings on stem cell research are discussed, bringing together basic and applied research with the newest insights in stem cell therapy.
Isolation and Bioactivity of Secondary Metabolites from Solid Culture of the Fungus, Alternaria sonchi
Authors: Dalinova Anna , Chisty Leonid , Kochura Dmitry , Garnyuk Varvara , Petrova Maria , Prokofieva Darya , Yurchenko Anton , Dubovik Vsevolod , Ivanov Alexander , Smirnov Sergey , Zolotarev Andrey , Berestetskiy Alexander
Published: January 2020

The fungus, Alternaria sonchi is considered to be a potential agent for the biocontrol of perennial sowthistle (Sonchus arvensis). A new chlorinated xanthone, methyl 8-hydroxy-3-methyl-4-chloro-9-oxo-9H-xanthene-1-carboxylate (1) and a new benzophenone derivative, 5-chloromoniliphenone (2), were isolated together with eleven structurally related compounds (3–13) from the solid culture of the fungus, which is used for the production of bioherbicidal inoculum of A. sonchi. Their structures were determined by spectroscopic (mostly by NMR and MS) methods. Alternethanoxins A and B, which were reported in A. sonchi earlier, were re-identified as moniliphenone and pinselin, respectively. The isolated compounds were tested for phytotoxic, antimicrobial, insecticidal, cytotoxic and esterase-inhibition activities. They did not demonstrate high phytotoxicity (lesions up to 2.5 mm in diameter/length at a concentration of 2 mg/mL) when tested on leaf disks/segments of perennial sowthistle (Sonchus arvensis) and couch grass (Elytrigia repens). They did not possess acute toxicity to Paramecium caudatum, and showed moderate to low cytotoxicity (IC50 > 25 µg/mL) for U937 and K562 tumor cell lines. However, chloromonilicin and methyl 3,8-dihydroxy-6-methyl-4-chloro-9-oxo-9H-xanthene-1-carboxylate (4) were shown to have antimicrobial properties with MIC 0.5–5 µg/disc. Compound 4 and chloromonilinic acid B were found to have contact insecticidal activity to wheat aphid (Schizaphis graminum) at 1 mg/mL. Compounds 2 and methyl 3,8-dihydroxy-6-methyl-9-oxo-9H-xanthene-1-carboxylate displayed selective carboxylesterase inhibition activity at concentration of 100 µg/mL. Therefore, the waste solid substrate for production of A. sonchi spores can be re-utilized for the isolation of a number of valuable natural products.
Serine Protease Inhibitors—New Molecules for Modification of Polymeric Biomaterials
Authors: Szałapata Katarzyna , Osińska-Jaroszuk Monika , Kapral-Piotrowska Justyna , Pawlikowska-Pawlęga Bożena , Łopucki Rafał , Mroczka Robert , Jarosz-Wilkołazka Anna
Published: January 2020

Three serine protease inhibitors (AEBSF, soy inhibitor, α1-antitrypsin) were covalently immobilized on the surface of three polymer prostheses with the optimized method. The immobilization efficiency ranged from 11 to 51%, depending on the chosen inhibitor and biomaterial. The highest activity for all inhibitors was observed in the case of immobilization on the surface of the polyester Uni-Graft prosthesis, and the preparations obtained showed high stability in the environment with different pH and temperature values. Modification of the Uni-Graft prosthesis surface with the synthetic AEBSF inhibitor and human α1-antitrypsin inhibited the adhesion and multiplication of Staphylococcus aureus subs. aureus ATCC® 25923TM and Candida albicans from the collection of the Department of Genetics and Microbiology, UMCS. Optical profilometry analysis indicated that, after the immobilization process on the surface of AEBSF-modified Uni-Graft prostheses, there were more structures with a high number of protrusions, while the introduction of modifications with a protein inhibitor led to the smoothing of their surface.
The Application of Pollen as a Functional Food and Feed Ingredient—The Present and Perspectives
Authors: Kostić Aleksandar Ž. , Milinčić Danijel D. , Barać Miroljub B. , Ali Shariati Mohammad , Tešić Živoslav Lj. , Pešić Mirjana B.
Published: January 2020

Pollen is recognized as an excellent dietary supplement for human nutrition, which is why it can be found in different forms on the market (granules, capsules, tablets, pellets, and powders). But, the digestibility of pollen’s nutrients is strongly affected by the presence of a pollen shell, which can decrease the bioavailability of nutrients by 50% and more. Since consumers have become more aware of the benefits of a healthy diet and the necessity to improve pollen digestibility, different pollen-based functional food products have been developed and extensive studies were done to estimate the beneficial effects of pollen-based feed on animal growth, health, and rigor mortise stage. Considering the positive effects of pollen nutrients and phytometabolites on human and animal health, the aim of this paper was to give an overview of recent achievements in the application of pollen in the formulation of functional food and animal diets. Special attention was paid to the effects of pollen’s addition on the nutritional, functional, techno-functional, and sensory properties of the new formulated food products. Anti-nutritional properties of pollen were also discussed. This review points out the benefits of pollen addition to food and feed and the possible directions in the further development of functional food and feed for the wellbeing of everyone.
Both Matricaria chamomilla and Metformin Extract Improved the Function and Histological Structure of Thyroid Gland in Polycystic Ovary Syndrome Rats through Antioxidant Mechanism
Authors: Alahmadi Ahlam Abdulaziz , Alzahrani Areej Ali , Ali Soad Shaker , Alahmadi Bassam Abdulaziz , Arab Rana Ali , El-Shitany Nagla Abd El-Aziz
Published: January 2020

There is increasing proof that polycystic ovary syndrome (PCOS) is associated with the increased frequency of thyroid disturbances. Chamomile (Matricaria chamomilla L.) herb and metformin showed therapeutic efficacy against polycystic ovary syndrome (PCOS). This study aimed to investigate the possible therapeutic effect of both chamomile flower extract and metformin against thyroid damage associated with PCOS in rats. The PCOS model was developed in rats by injecting estradiol valerate, and it was confirmed to be associated with thyroid hypofunction biochemically and pathologically. Treatment of PCOS rats with both chamomile extract and metformin resulted in an improvement in serum level of thyroid hormones (TSH, p < 0.01; T3 and T4, p < 0.05) and the disappearance of most thyroid gland pathological changes demonstrated by light and electron microscopes. They also reduced the level of serum estrogen (p < 0.01). Both chamomile extract and metformin decreased MDA (p < 0.05) and increased GPx and CAT (p < 0.01). Only chamomile extract increased GSH (p < 0.01). Both treatments reduced the apoptotic death of thyroid cells as noted by the reduction of caspase-3 immunoexpression (p < 0.01). In conclusion, both Matricaria chamomilla extract and metformin ameliorated hypothyroidism associated with PCOS through an antioxidant and antiapoptotic mechanism.
Differential Expression of CD31 and Von Willebrand Factor on Endothelial Cells in Different Regions of the Human Brain: Potential Implications for Cerebral Malaria Pathogenesis
Authors: Mbagwu Smart Ikechukwu , Filgueira Luis
Published: January 2020

Cerebral microvascular endothelial cells (CMVECs) line the vascular system of the brain and are the chief cells in the formation and function of the blood brain barrier (BBB). These cells are heterogeneous along the cerebral vasculature and any dysfunctional state in these cells can result in a local loss of function of the BBB in any region of the brain. There is currently no report on the distribution and variation of the CMVECs in different brain regions in humans. This study investigated microcirculation in the adult human brain by the characterization of the expression pattern of brain endothelial cell markers in different brain regions. Five different brain regions consisting of the visual cortex, the hippocampus, the precentral gyrus, the postcentral gyrus, and the rhinal cortex obtained from three normal adult human brain specimens were studied and analyzed for the expression of the endothelial cell markers: cluster of differentiation 31 (CD31) and von-Willebrand-Factor (vWF) through immunohistochemistry. We observed differences in the expression pattern of CD31 and vWF between the gray matter and the white matter in the brain regions. Furthermore, there were also regional variations in the pattern of expression of the endothelial cell biomarkers. Thus, this suggests differences in the nature of vascularization in various regions of the human brain. These observations also suggest the existence of variation in structure and function of different brain regions, which could reflect in the pathophysiological outcomes in a diseased state.
5-Hydroxymethylfurfural and Furfural Base-Free Oxidation over AuPd Embedded Bimetallic Nanoparticles
Authors: P. Ferraz Camila , Costa Natalia J. S. , Teixeira-Neto Erico , Teixeira-Neto Ângela A. , Liria Cleber W. , Thuriot-Roukos Joëlle , Machini M. Teresa , Froidevaux Rénato , Dumeignil Franck , Rossi Liane M. , Wojcieszak Robert
Published: January 2020

The heterogeneous catalytic partial oxidation of alcohols and aldehydes in the liquid phase usually needs the addition of a homogeneous base, which in turn makes the products’ recovery cumbersome, and can further induce undesired side reactions. In the present work, we propose the use of novel catalysts based on metallic Au, Pd and bimetallic AuPd nanoparticles embedded in a titanosilicate matrix. The as-prepared catalysts showed good efficiency in the base-free partial oxidation of furfural and 5-hydroxymethylfurfural. Au4Pd1@SiTi catalyst showed high selectivity (78%) to monoacids (namely, 5-formyl-2-furancarboxylic acid and 5-hydroxymethyl-2-furancarboxylic acid) at 50% 5-hydroxymethylfurfural (HMF) conversion. The selectivity even reached 83% in the case of furfural oxidation to furoic acid (at 50% furfural conversion). The performances of the catalysts strongly depended on the Au–Pd ratio, with an optimal value of 4:1. The pH of the solution was always below 3.5 and no leaching of metals was observed, confirming the stabilization of the metal nanoparticles within the titanosilicate host matrix.
Interfacial Biocatalytic Performance of Nanofiber-Supported β-Galactosidase for Production of Galacto-Oligosaccharides
Authors: Misson Mailin , Jin Bo , Dai Sheng , Zhang Hu
Published: January 2020

Molecular distribution, structural conformation and catalytic activity at the interface between enzyme and its immobilising support are vital in the enzymatic reactions for producing bioproducts. In this study, a nanobiocatalyst assembly, β-galactosidase immobilized on chemically modified electrospun polystyrene nanofibers (PSNF), was synthesized for converting lactose into galacto-oligosaccharides (GOS). Characterization results using scanning electron microscopy (SEM) and fluorescence analysis of fluorescein isothiocyanat (FITC) labelled β-galactosidase revealed homogenous enzyme immobilization, thin layer structural conformation and biochemical functionalities of the nanobiocatalyst assembly. The β-galactosidase/PSNF assembly displayed enhanced enzyme catalytic performance at a residence time of around 1 min in a disc-stacked column reactor. A GOS yield of 41% and a lactose conversion of 88% was achieved at the initial lactose concentration of 300 g/L at this residence time. This system provided a controllable contact time of products and substrates on the nanofiber surface and could be used for products which are sensitive to the duration of nanobiocatalysis.
Mitochondrial Fusion Via OPA1 and MFN1 Supports Liver Tumor Cell Metabolism and Growth
Authors: Li Meng , Wang Ling , Wang Yijin , Zhang Shaoshi , Zhou Guoying , Lieshout Ruby , Ma Buyun , Liu Jiaye , Qu Changbo , Verstegen Monique M. A. , Sprengers Dave , Kwekkeboom Jaap , van der Laan Luc J. W. , Cao Wanlu , Peppelenbosch Maikel P. , Pan Qiuwei
Published: January 2020

Metabolic reprogramming universally occurs in cancer. Mitochondria act as the hubs of bioenergetics and metabolism. The morphodynamics of mitochondria, comprised of fusion and fission processes, are closely associated with mitochondrial functions and are often dysregulated in cancer. In this study, we aim to investigate the mitochondrial morphodynamics and its functional consequences in human liver cancer. We observed excessive activation of mitochondrial fusion in tumor tissues from hepatocellular carcinoma (HCC) patients and in vitro cultured tumor organoids from cholangiocarcinoma (CCA). The knockdown of the fusion regulator genes, OPA1 (Optic atrophy 1) or MFN1 (Mitofusin 1), inhibited the fusion process in HCC cell lines and CCA tumor organoids. This resulted in inhibition of cell growth in vitro and tumor formation in vivo, after tumor cell engraftment in mice. This inhibitory effect is associated with the induction of cell apoptosis, but not related to cell cycle arrest. Genome-wide transcriptomic profiling revealed that the inhibition of fusion predominately affected cellular metabolic pathways. This was further confirmed by the blocking of mitochondrial fusion which attenuated oxygen consumption and cellular ATP production of tumor cells. In conclusion, increased mitochondrial fusion in liver cancer alters metabolism and fuels tumor cell growth.
CD276-Positive Circulating Endothelial Cells Do Not Predict Response to Systemic Therapy in Advanced Colorectal Cancer
Authors: Gootjes Elske C. , Kraan Jaco , Buffart Tineke E. , Bakkerus Lotte , Zonderhuis Barbara M. , Verhoef Cornelis , Verheul Henk M.W. , Sleijfer Stefan
Published: January 2020

CD276 can discriminate between tumor derived and normal CECs (circulating endothelial cells). We evaluated whether CD276+CEC is a clinically relevant biomarker to predict response to palliative systemic therapy in patients with metastatic colorectal cancer (mCRC). Samples were prospectively collected from patients with mCRC enrolled in the ORCHESTRA trial (NCT01792934). At baseline and after three cycles of 5-fluorouracil/leucovorin and oxaliplatin ± bevacizumab, CECs were measured by flowcytometry (CD34+CD45negCD146+DNA+; and CD276+). A clinically relevant cut-off value of (CD276+)CECs was determined as 100% sensitivity (and 80% specificity in 95% confidence interval) identifying patients with progressive disease within 6 months. There were 182 baseline samples and 133 follow up samples available for analysis. CEC and CD276+CEC counts significantly increased during treatment from 48 to 90 CEC/4 mL (p = 0.00) and from 14 to 33 CD276+CEC/4 mL (p = 0.00) at baseline and at first evaluation, respectively. CEC and CD276+CEC counts were not predictive for poor response (area under the curve (AUC) 0.53 for CEC and AUC 0.52 for CD276+CEC). Despite numerical changes during therapy, CEC and CD276+CEC counts do not adequately predict poor response to first line palliative systemic therapy in patients with mCRC.
TRPC Channels in the SOCE Scenario
Authors: Lopez Jose J. , Jardin Isaac , Sanchez-Collado Jose , Salido Ginés M. , Smani Tarik , Rosado Juan A.
Published: January 2020

Transient receptor potential (TRP) proteins form non-selective Ca2+ permeable channels that contribute to the modulation of a number of physiological functions in a variety of cell types. Since the identification of TRP proteins in Drosophila, it is well known that these channels are activated by stimuli that induce PIP2 hydrolysis. The canonical TRP (TRPC) channels have long been suggested to be constituents of the store-operated Ca2+ (SOC) channels; however, none of the TRPC channels generate Ca2+ currents that resemble ICRAC. STIM1 and Orai1 have been identified as the components of the Ca2+ release-activated Ca2+ (CRAC) channels and there is a body of evidence supporting that STIM1 is able to gate Orai1 and TRPC1 in order to mediate non-selective cation currents named ISOC. STIM1 has been found to interact to and activate Orai1 and TRPC1 by different mechanisms and the involvement of TRPC1 in store-operated Ca2+ entry requires both STIM1 and Orai1. In addition to the participation of TRPC1 in the ISOC currents, TRPC1 and other TRPC proteins might play a relevant role modulating Orai1 channel function. This review summarizes the functional role of TRPC channels in the STIM1–Orai1 scenario.
Methods for Early Detection of Microbiological Infestation of Buildings Based on Gas Sensor Technologies
Authors: Garbacz Monika , Malec Agnieszka , Duda-Saternus Sylwia , Suchorab Zbigniew , Guz Łukasz , Łagód Grzegorz
Published: January 2020

In this review, the problem of microbiological infestation of buildings was discussed. The techniques of detection were described as well, with special attention drawn to the rapid-early detection methods based on gas sensor arrays. The physical and chemical conditions of the building environment conducive to the development of microorganisms and the technical conditions influencing the problem of microbiological infestation were investigated. Additionally, the harmful effects on human health caused by the microbiological contamination were discussed, with a short review of particular groups of microorganisms causing sick building syndrome. Among the detection techniques, the traditional microbiological techniques as well as the molecular and chemical methods were presented. Different designs of the gas sensor arrays together with the various techniques of analyzing the received multidimensional signal were described, analyzed, and compared in detail.
An Investigation of the Mechanical and Physical Characteristics of Cement Paste Incorporating Different Air Entraining Agents using X-ray Micro-Computed Tomography
Authors: Abd Elrahman Mohamed , El Madawy Mohamed E. , Chung Sang-Yeop , Majer Stanisław , Youssf Osama , Sikora Pawel
Published: January 2020

Improving the thermal insulation properties of cement-based materials is the key to reducing energy loss and consumption in buildings. Lightweight cement-based composites can be used efficiently for this purpose, as a structural material with load bearing ability or as a non-structural one for thermal insulation. In this research, lightweight cement pastes containing fly ash and cement were prepared and tested. In these mixes, three different techniques for producing air voids inside the cement paste were used through the incorporation of aluminum powder (AL), air entraining agent (AA), and hollow microspheres (AS). Several experiments were carried out in order to examine the structural and physical characteristics of the cement composites, including dry density, compressive strength, porosity and absorption. A Hot Disk device was used to evaluate the thermal conductivity of different cement composites. In addition, X-ray micro-computed tomography (micro-CT) was adopted to investigate the microstructure of the air-entrained cement pastes and the spatial distribution of the voids inside pastes without destroying the specimens. The experimental results obtained showed that AS specimens with admixture of hollow microspheres can improve the compressive strength of cement composites compared to other air entraining admixtures at the same density level. It was also confirmed that the incorporation of aluminum powder creates large voids, which have a negative effect on specimens’ strength and absorption.
Pseudorandom Number Generator (PRNG) Design Using Hyper-Chaotic Modified Robust Logistic Map (HC-MRLM)
Authors: Irfan Muhammad , Ali Asim , Khan Muhammad Asif , Ehatisham-ul-Haq Muhammad , Mehmood Shah Syed Nasir , Saboor Abdul , Ahmad Waqar
Published: January 2020

Robust chaotic systems, due to their inherent properties of mixing, ergodicity, and larger chaotic parameter space, constitute a perfect candidate for cryptography. This paper reports a novel method to generate random numbers using modified robust logistic map (MRLM). The non-smooth probability distribution function of robust logistic map (RLM) trajectories gives an un-even binary distribution in randomness test. To overcome this disadvantage in RLM, control of chaos (CoC) is proposed for smooth probability distribution function of RLM. For testing the proposed design, cryptographic random numbers generated by MRLM were vetted with National Institute of Standards and Technology statistical test suite (NIST 800-22). The results showed that proposed MRLM generates cryptographically secure random numbers (CSPRNG).
Exploiting Grasshopper and Cuckoo Search Bio-Inspired Optimization Algorithms for Industrial Energy Management System: Smart Industries
Authors: Ullah Ibrar , Hussain Irshad , Singh Madhusudan
Published: January 2020

Industries are consuming more than 27% of the total generated energy in the world, out of which 50% is used by different machines for processing, producing, and assembling various goods. Energy shortage is a major issue of this biosphere. To overcome energy scarcity, a challenging task is to have optimal use of existing energy resources. An efficient and effective mechanism is essential to optimally schedule the load units to achieve three objectives: minimization of the consumed energy cost, peak-to-average power ratio, and consumer waiting time due to scheduling of the load. To achieve the aforementioned objectives, two bio-inspired heuristic techniques—Grasshopper-Optimization Algorithm and Cuckoo Search Optimization Algorithm—are analyzed and simulated for efficient energy use in an industry. We considered a woolen mill as a case study, and applied our algorithms on its different load units according to their routine functionality. Then we scheduled these load units by proposing an efficient energy management system (EMS). We assumed automatic operating machines and day-ahead pricing schemes in our EMS.
Characterization and Development of Genomic SSRs in Pecan (Carya illinoinensis)
Authors: Zhang Chengcai , Yao Xiaohua , Ren Huadong , Chang Jun , Wu Jun , Shao Weizhong , Fang Qing
Published: January 2020

Research Highlights: The distribution of simple sequence repeat (SSR) motifs in two draft genomes of pecan was evaluated. Sixty-six SSR loci were validated by PCR amplification in pecan. Twenty-two new development markers can be used for genetic study in genus Carya. Background and Objectives: Pecan has good nutritional and health benefits and is an important crop worldwide. However, the genetic research in this species is insufficient. One of the main reasons for this is the lack of enough accurate, convenient, and economical molecular markers. Among different marker types, SSR loci are enormously useful in genetic studies. However, the number of SSRs in C. illinoinensis (Wangenh.) K. Koch is limited. Materials and Methods: The distribution of SSR motifs in the pecan genome was analyzed. Then, the primers for each SSR were designed. To evaluate their availability, 74 SSR loci were randomly selected and amplified in pecan. Finally, 22 new SSRs and eight former ones were picked to evaluate the genetic diversity in 60 pecan genotypes and to determine their transferability in other Carya species. Results: 145,714 and 143,041 SSR motifs were obtained from two draft genomes of ‘87MX3-2’ and ‘Pawnee’, respectively. In total, 9145 candidate primers were obtained. Sixty-six (89.19%) primers amplified the target products. Among the 30 SSRs, 29 loci showed polymorphism in 60 pecan genotypes. The polymorphic information content (PIC) values ranged from 0.012 to 0.906. In total, 26, 25, and 22 SSRs can be used in C. cathayensis Sarg., C. dabieshanensis W. C. Cheng & R. H. Chang, and C. hunanensis W.C. Liu, respectively. Finally, the dendrogram of all individuals was constructed. The results agree with the geographic origin of the four species and the pedigree relationships between different pecan cultivars. Conclusions: The characterization of SSRs in the pecan genome and the new SSRs will promote the progress of genetic study and breeding in pecan, as well as other species of genus Carya.
Sparse Pinus Tabuliformis Stands Have Higher Canopy Transpiration Than Dense Stands Three Decades After Thinning
Authors: Chen Zuosinan , Zhang Zhiqiang , Chen Lixin , Cai Yongmao , Zhang Haiquan , Lou Junpeng , Xu Zhou , Xu Hang , Song Conghe
Published: January 2020

Hydrological effects of forest thinning have been studied at small watershed scales using the paired watershed approach since the 1920s. However, how forest transpiration, a critical component of evapotranspiration, changes decades after thinning is not well understood despite its importance for modifying drought resilience of forest ecosystem under climate change. In a semi-arid mountainous area of northern China, we measured growing season sap flow of Pinus tabuliformis, a widely planted afforestation species, in 44-year-old monoculture plantation stands with low (983 stems ha−1), medium (1688 stems ha−1), and high (2160 stems ha−1) density. Three decades after thinning, diameters at breast height (DBH) were larger in sparse stands than in dense stands. While its relation with sapwood area was density independent, the accompanying high sapwood area at the tree level for sparse stands resulted the highest stand sapwood area in the medium density stand (33.26 m2 ha−1), rather than in the high density stand (29.84 m2 ha−1). Similar to short-term studies, sparse stands demonstrated higher sensitivity to climatic fluctuations and drought depressions than dense ones. Nevertheless, stand density had no effect on the isohydric strategy of Pinus tabuliformis. Contrary to the positive relation between stand density and stand canopy transpiration soon after thinning, sparse stands exhibited higher growing season canopy transpiration than dense stands three decades later. In the dry year 2014, these density differences were relatively most pronounced, with July-September transpiration totals of 56.7 mm, 31.1 mm, and 22.1 mm in the low, medium, and high density stands, respectively. Our findings highlighted that stand density was not an appropriate indicator of thinned forest transpiration over long time scales. Interactions between soil droughts and thinning on forest transpiration need to be further clarified, especially in longer periods of time.
Characteristics of Bread Made of Various Substitution Ratios of Bran Pulverized by Hammer Mill or Jet Mill
Authors: Lee Dabeen , Kim Mi Jeong , Kwak Han Sub , Kim Sang Sook
Published: January 2020

The physicochemical and antioxidant properties of dough and bread were measured in wheat flours substituted with two types of bran (HMB: bran pulverized by a hammer mill and JMB: bran pulverized by a jet mill) at various ratios (0%, 5%, 10%, 15%, 20%, and 25%) of substitution. The particle size of hammer mill bran (HMB) (119.71 µm) was larger than that of jet mill bran (JMB) (25.78 µm). Wheat flours substituted with HMB contained more total dietary fiber than those with JMB. A significant increase of water absorption and dough development time in Mixolab® analysis was observed depending on the level of HMB or JMB substitution. The breads made with HMB or JMB (5% or 10%) showed a higher specific volume and lower crumb hardness than the control bread. However, breads made with ≥15% HMB or JMB had a decreased specific volume and increased crumb hardness. Overall, breads made with wheat flour substituted with 5%–10% HMB or JMB were of a higher bread quality and had more antioxidant properties.
Impact of Type and Enzymatic/High Pressure Treatment of Milk on the Quality and Bio-Functional Profile of Yoghurt
Authors: Tsevdou Maria , Theodorou Georgios , Pantelaiou Sofia , Chatzigeorgiou Artemis , Politis Ioannis , Taoukis Petros
Published: January 2020

The objective of the present study was to investigate the effect of the high pressure (HP) processing and transglutaminase (TGase) treatment of bovine (cow) or ovine (sheep) milk, when applied individually or sequentially, on the quality parameters and anti-hypertensive and immunomodulatory properties of yoghurt. Low-fat (2% w/w) bovine or ovine milk samples were used. Results showed that HP treatment of milk led to acid gels with equivalent quality attributes to thermal treatment, with the more representative attributes being whey separation and firmness, which ranged from 47.5% to 49.8% and 23.8% to 32.2% for bovine and ovine yoghurt, respectively, and 74.3–89.0 g and 219–220 g for bovine and ovine yoghurt, respectively. On the other hand, TGase treatment of milk, solely or more effectively following HP processing, resulted in the improvement of the textural attributes of yoghurt and reduced whey separation, regardless of milk type, exhibiting values of 32.9% and 8.7% for the whey separation of bovine and ovine yoghurt, respectively, and 333 g and 548 g for the firmness of bovine and ovine yoghurt, respectively. The HP processing and TGase treatment of milk led to the preservation or improvement of the anti-hypertensive activity of the samples, especially in the case in which ovine milk was used, with Inhibitory activity of Angiotensin Converting Enzyme (IACE) values of 76.9% and 88.5% for bovine and ovine yoghurt, respectively. The expression of pro-inflammatory genes decreased and that of anti-inflammatory genes increased in the case of samples from HP-processed and/or TGase-treated milk as compared to the corresponding expressions for samples from thermally treated milk. Thus, it can be stated that, apart from the quality improvement, HP processing and TGase treatment of milk may lead to the enhancement of the bio-functional properties of low-fat yoghurt made from either bovine or ovine milk.
Acacia Gum as a Natural Anti-Plasticizer for the Production of Date Syrup Powder: Sorption Isotherms, Physicochemical Properties, and Data Modeling
Authors: Mansoori Nasim , Majzoobi Mahsa , Gavahian Mohsen , Badii Fojan , Farahnaky Asgar
Published: January 2020

The thermoplastic and hygroscopic behaviors of date syrup (DS) challenge the DS drying process. In this context, DS was mixed with 30%, 40%, 50%, and 60% acacia gum (AG) and subjected to a drum dryer. The chemical composition, bulk density (pb), caking degree (CD), glass transition temperature (Tg), and color values of DS powders were studied. The sorption isotherms were also obtained and compared to that of those predicted by mathematical models. According to the results, increasing the AG concentration enhanced the moisture content, pb, brightness, and Tg while it reduced the CD and equilibrium moisture sorption. All DS powders had type III isotherm behavior, i.e., similar to high-sugar foods. Guggenheim-Anderson-de Boer (GAB) and Peleg models were found to be suitable for fitting the experimental data and these models explained the monolayer moisture content decrease with increasing AG concentration. These results of the present study, for the first time, verified that the AG can be used as a natural anti-plasticizer agent for DS powder production.
Genomic Selection for Optimum Index with Dry Biomass Yield, Dry Mass Fraction of Fresh Material, and Plant Height in Biomass Sorghum
Authors: Habyarimana Ephrem , Lopez-Cruz Marco , Baloch Faheem S.
Published: January 2020

Sorghum is one of the world’s major crops, expresses traits for resilience to climate change, and can be used for several purposes including food and clean fuels. Multiple-trait genomic prediction and selection models were implemented using genotyping-by-sequencing single nucleotide polymorphism markers and phenotypic data information. We demonstrated for the first time the efficiency genomic selection modelling of index selection including biofuel traits such as aboveground biomass yield, plant height, and dry mass fraction of the fresh material. This work also sheds light, for the first time, on the promising potential of using the information from the populations grown from seed to predict the performance of the populations regrown from the rhizomes—even two winter seasons after the original trial was sown. Genomic selection modelling of the optimum index selection including the three traits of interest (plant height, aboveground dry biomass yield, and dry mass fraction of fresh mass material) was the most promising. Since the plant characteristics evaluated herein are routinely measured in cereal and other plant species of agricultural interest, it can be inferred that the findings can be transferred in other major crops.
Competition Seriousness and Competition Level Modulate Testosterone and Cortisol Responses in Soccer Players
Authors: Jiménez Manuel , Alvero-Cruz José Ramón , Solla Juan , García-Bastida Jorge , García-Coll Virginia , Rivilla Iván , Ruiz Enrique , García-Romero Jerónimo , Carnero Elvis A. , Clemente-Suárez Vicente Javier
Published: January 2020

This study aimed to analyze the modulating effect of competition seriousness and competition level in the testosterone and cortisol responses in professional soccer player. Ninety five (95) soccer players were included in this study (professional, n = 39; semiprofessional, n = 27; amateur, n = 29) before and after training, friendly game and official games. Repeated measures ANOVA showed higher testosterone levels (F(1,89) = 134, p < 0.0001, η2p = 0.75) in professional soccer players, when compared with semiprofessional (p < 0.0001) or amateur athletes (p < 0.0001). After winning a competition game an increase in testosterone levels was observed in professionals (t = −3.456, p < 0.001), semiprofessionals (t = −4.400, p < 0.0001), and amateurs (t = −2.835, p < 0.009). In contrast, this momentary hormonal fluctuation was not observed after winning a friendly game or during a regular training day. Additionally, statistical analysis indicated that cortisol levels were lower in professional (t = −3.456, p < 0.001) and semiprofessional athletes (t = −4.400, p < 0.0001) than in amateurs (t = −2.835, p < 0.009). In soccer players a rise in testosterone was only observable when the team was faced with an actual challenge but did not support a different response between categories. Thus, the desire to achieve a goal (and keep the social status) may be one of the key reasons why testosterone levels rise promptly. Conversely, testosterone did not change after friendly games, which suggests these situations are not real goals and the players do not perceive an actual threat (in terms of dominance) more than the preparation for their next competitive game.