bluuebunny/crossref_metadata_2025 huggingface dataset:
Research Progress of Electroplated Nanotwinned Copper in Microelectronic Packaging
Authors: Chen Ke-Xin , Gao Li-Yin , Li Zhe , Sun Rong , Liu Zhi-Quan
Published: June 2023

Copper is the most common interconnecting material in the field of microelectronic packaging, which is widely used in advanced electronic packaging technologies. However, with the trend of the miniaturization of electronic devices, the dimensions of interconnectors have decreased from hundreds of microns to tens of or even several microns, which has brought serious reliability issues. As a result, nanotwinned copper (nt-Cu) has been proposed as a potential candidate material and is being certified progressively. Firstly, the physical properties of nt-Cu have been widely studied. Notably, the higher thermal stability and oxidation resistance of the (111) texture causes nt-Cu to maintain excellent physical properties under high-temperature serving conditions. Secondly, recent works on the electrolyte and electroplating processes of nt-Cu on wafer substrates are summarized, focusing on how to reduce the thickness of the transition layer, improve the twin density, and achieve complicated pattern filling. Thirdly, nt-Cu can effectively eliminate Kirkendall voids when it serves as UBM or a CuP. Additionally, the high (111) texture can control the preferred orientation of interfacial intermetallic compounds (IMCs) at the Cu–Sn interface, which should be helpful to improve the reliability of solder joints. nt-Cu has superior electromigration resistance and antithermal cycling ability compared to ordinary copper RDLs and TSVs. Above all, nt-Cu has attracted much attention in the field of microelectronic packaging in recent years. The preparation–performance–reliability interrelationship of nt-Cu is summarized and displayed in this paper, which provides a solid theoretical basis for its practical applications.
Design of Pt-Sn-Zn Nanomaterials for Successful Methanol Electrooxidation Reaction
Authors: Milošević Dragana , Stevanović Sanja , Tripković Dušan , Vukašinović Ivana , Maksimović Vesna , Ćosović Vladan , Nikolić Nebojša D.
Published: June 2023

This work highlights the potential for the synthesis of new PtSnZn catalysts with enhanced efficiency and durability for methanol oxidation reaction (MOR) in low-temperature fuel cells. In this research, PtZn and PtSnZn nanoparticles deposited on high surface area Vulcan XC-72R Carbon support were created by a microwave-assisted polyol method. The electrochemical performances of synthesized catalysts were analyzed by cyclic voltammetry and by the electrooxidation of adsorbed CO and the chronoamperometric method. The physicochemical properties of obtained catalysts were characterized by transmission electron microscopy (TEM), thermogravimetric (TGA) analysis, energy dispersive spectroscopy (EDS) and by X-ray diffraction (XRD). The obtained findings showed the successful synthesis of platinum-based catalysts. It was established that PtSnZn/C and PtZn/C catalysts have high electrocatalytic performance in methanol oxidation reactions. Catalysts stability tests were obtained by chronoamperometry. Stability tests also confirmed decreased poisoning and indicated improved stability and better tolerance to CO-like intermediate species. According to activity and stability measurements, the PtSnZn/C catalyst possesses the best electrochemical properties for the methanol oxidation reaction. The observed great electrocatalytic activity in the methanol oxidation reaction of synthesized catalysts can be attributed to the beneficial effects of microwave synthesis and the well-balanced addition of alloying metals in PtSnZn/C catalysts.
Multi-Omics Data Analysis Identifies Prognostic Biomarkers across Cancers
Authors: Demir Karaman Ezgi , Işık Zerrin
Published: June 2023

Combining omics data from different layers using integrative methods provides a better understanding of the biology of a complex disease such as cancer. The discovery of biomarkers related to cancer development or prognosis helps to find more effective treatment options. This study integrates multi-omics data of different cancer types with a network-based approach to explore common gene modules among different tumors by running community detection methods on the integrated network. The common modules were evaluated by several biological metrics adapted to cancer. Then, a new prognostic scoring method was developed by weighting mRNA expression, methylation, and mutation status of genes. The survival analysis pointed out statistically significant results for GNG11, CBX2, CDKN3, ARHGEF10, CLN8, SEC61G and PTDSS1 genes. The literature search reveals that the identified biomarkers are associated with the same or different types of cancers. Our method does not only identify known cancer-specific biomarker genes, but also proposes new potential biomarkers. Thus, this study provides a rationale for identifying new gene targets and expanding treatment options across cancer types.
Effect of Temperature on the Morphology and Corrosion Resistance of Modified Boron Nitride Nanosheets Incorporated into Steel Phosphate Coating
Authors: Muhammad Mustafa , Ma Ruina , Du An , Fan Yongzhe , Zhao Xue , Jun Niu , Cao Xiaoming
Published: June 2023

In this study, the impact of various temperatures (25 °C, 45 °C, and 55 °C) on q235 steel specimens was studied to analyze the influence on the morphology and corrosion resistance of modified boron nitride nanosheets incorporated into the phosphate coating. The morphology and surface modification of the coating were characterized by scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR). SEM results showed that the S-45 steel sample produced a higher number of nucleation sites and reduced grain size with a denser, more robust, and more corrosion-resistant phosphate coating compared to the S-25 and S-55 samples, respectively. The potentiodynamic polarization results demonstrate that the S-45 steel sample exhibited the best corrosion resistance performance with an electric current density of 5.48 × 10−8 A/cm2, an order of magnitude lower than the S-25 and S-55 samples, respectively. The coating weight results showed that the S-45 steel specimen achieved the densest and most uniform coating (32.14 g/m2).
Reconfigurable Terahertz Spatial Deflection Varifocal Metamirror
Authors: Fang Jianhui , Zhong Renbin , Xu Boli , Zhang Huimin , Wu Qian , Guo Benzheng , Wang Jianian , Wu Zhenhua , Hu Min , Zhang Kaichun , Liu Diwei
Published: June 2023

A traditional optical lens usually has a fixed focus, and its focus controlling relies on a bulky lens component, which makes integration difficult. In this study, we propose a kind of terahertz spatial varifocal metamirror with a consistent metal–graphene unit structure whose focus can be flexibly adjusted. The focus deflection angle can be theoretically defined by superimposing certain encoded sequence on it according to Fourier convolution theorem. The configurable metamirror allows for the deflection of the focus position in space. The proposed configuration approach presents a design concept and offers potential advancements in the field of developing novel terahertz devices.
Dietary Fiber from Soybean (Glycine max) Husk as Fat and Phosphate Replacer in Frankfurter Sausage: Effect on the Nutritional, Physicochemical and Nutraceutical Quality
Authors: Araujo-Chapa Ana P. , Urías-Orona Vania , Niño-Medina Guillermo , Muy-Rangel Dolores , de la Garza Ana Laura , Castro Heriberto
Published: June 2023

The objective of the present study was to evaluate the effect of dietary fiber from soybean (glycine max) husk as fat and phosphate replacer on the nutritional, physicochemical, and nutraceutical quality of Frankfurter sausage. A traditional formulation was used for the pork-based sausage and three treatments were established: control treatment (CT), sausage without SHDF; treatment 1 (T1), sausage and 1% SHDF; treatment 2 (T2), sausage and 1.5% SHDF. T2 showed the best nutritional contribution of the treatments, significantly favoring a lower content of fat and sodium, thus increasing the contribution of dietary fiber and calcium. A positive effect of SHDF on the water-holding capacity of the treatments was also observed. In addition, T2 remained stable during storage, while T1 and CT showed significantly reduced water-holding capacities of approximately 5%; this was in turn linked to hardness, as it was observed that on day 7 of storage, 27% less force was required to deform the T2 sausages. Regarding color, no significant difference was observed with the addition of SHDF to the product. The results suggest that the dietary fiber extracted from soybean husks has potential for application in food and can be used as an ingredient to improve the functional and nutritional quality of Frankfurter sausages by reducing the content of fat and phosphates.
Electrospun Sandwich-like Structure of PVDF-HFP/Cellulose/PVDF-HFP Membrane for Lithium-Ion Batteries
Authors: Zi Xingfu , Wu Hongming , Song Jiling , He Weidi , Xia Lu , Guo Jianbing , Luo Sihai , Yan Wei
Published: June 2023

Cellulose membranes have eco-friendly, renewable, and cost-effective features, but they lack satisfactory cycle stability as a sustainable separator for batteries. In this study, a two-step method was employed to prepare a sandwich-like composite membrane of poly(vinylidene fluoride-co-hexafluoropropylene) (PVDF-HFP)/cellulose/ PVDF-HFP (PCP). The method involved first dissolving and regenerating a cellulose membrane and then electrospinning PVDF-HFP on its surface. The resulting PCP composite membrane exhibits excellent properties such as high porosity (60.71%), good tensile strength (4.8 MPa), and thermal stability up to 160 °C. It also has exceptional electrolyte uptake properties (710.81 wt.%), low interfacial resistance (241.39 Ω), and high ionic conductivity (0.73 mS/cm) compared to commercial polypropylene (PP) separators (1121.4 Ω and 0.26 mS/cm). Additionally, the rate capability (163.2 mAh/g) and cycling performance (98.11% after 100 cycles at 0.5 C) of the PCP composite membrane are superior to those of PP separators. These results demonstrate that the PCP composite membrane has potential as a promising separator for high-powered, secure lithium-ion batteries.
Water-Induced Regeneration of a 2,2-Diphenyl-1-picrylhydrazyl Radical after Its Scandium Ion-Promoted Electron-Transfer Disproportionation in an Aprotic Medium
Authors: Nakanishi Ikuo , Shoji Yoshimi , Ohkubo Kei , Ito Hiromu , Fukuzumi Shunichi
Published: June 2023

A neutral, stable radical, 2,2-diphenyl-1-picrylhydrazyl radical (DPPH•), has been frequently used to estimate the activity of antioxidants for more than 60 years. However, the number of reports about the effect of metal ions on the reactivity of DPPH• is quite limited. We have recently reported a unique electron-transfer disproportionation of DPPH• to produce the DPPH cations (DPPH+) and anions (DPPH−) upon the addition of scandium triflate [Sc(OTf)3 (OTf = OSO2CF3)] to an acetonitrile (MeCN) solution of DPPH•. The driving force of this reaction is suggested to be an interaction between DPPH– and Sc3+. In this study, it is demonstrated that the addition of H2O to the DPPH•–Sc(OTf)3 system in MeCN resulted in an increase in the absorption band at 519 nm due to DPPH•. This indicated that an electron-transfer comproportionation occurred to regenerate DPPH•. The regeneration of DPPH• was also confirmed by electron paramagnetic resonance (EPR) spectroscopy. The amount of DPPH• increased with an increasing amount of added H2O to reach a constant value. The detailed mechanism of regeneration of DPPH• was proposed based on the detailed spectroscopic and kinetic analyses, in which the reaction of DPPH+ with [(DPPH)2Sc(H2O)3]+ generated upon the addition of H2O to [(DPPH)2Sc]+ is the rate-determining step.
Optimizing the Extraction and Enrichment of Luteolin from Patrinia villosa and Its Anti-Pseudorabies Virus Activity
Authors: Fu Lian , Li Su , Men Xiaoyu , Cai Xiaojing , Wang Zhiying , Xu Yongkang , Ren Zhiyuan , Shao Yi , Zhu Yan
Published: June 2023

Luteolin from Patrinia villosa exhibits strong antiviral activity. Here, the conditions for extracting and enriching luteolin from P. villosa were optimized. Response surface methodology was used to determine the optimal extraction parameters in terms of reflux time, solvent ratio, extraction temperature, material-to-liquid ratio, and number of extractions. Thereafter, a macroporous resin method was used to enrich luteolin from P. villosa. Finally, the following optimal extraction and enrichment conditions were established: an extraction time of 43.00 min, a methanol/hydrochloric acid solvent ratio of 13:1, an extraction temperature of 77.60 °C, a material/liquid ratio of 1:22, and a total of two extractions. NKA-9 was determined to be the most appropriate resin for enrichment. The ideal adsorption conditions were as follows: a pH of 5.0, a temperature of 25 °C, an initial luteolin concentration of 19.58 µg/mL, a sample loading volume of 2.9 BV, and a sample loading rate of 2 BV/h. The ideal desorption conditions were as follows: distilled water, 30% ethanol and 80% ethanol elution, and 5 BV at a flow rate of 2 BV/h. After optimization, the enrichment recovery rate was 80.06% and the luteolin content increased 3.8-fold. Additionally, the enriched product exhibited a significant inhibitory effect on PRV (Porcine pseudorabies virus) in vitro and in vivo, providing data for developing and applying luteolin from P. villosa.
Nigella sativa-Manganese Ferrite-Reduced Graphene Oxide-Based Nanomaterial: A Novel Adsorbent for Water Treatment
Authors: Tara Nusrat , Abomuti May Abdullah , Alshareef F. M. , Abdullah Omeima , Allehyani Esam S. , Chaudhry Saif Ali , Oh Seungdae
Published: June 2023

In this study, a novel nanohybrid composite was fabricated via the incorporation of manganese ferrite (MnFe2O4) nanoparticles into the integrated surface of reduced graphene oxide (rGO) and black cumin seeds (BC). The nanohybrid composite was prepared by a simple co-precipitation method and characterized by several spectroscopic and microscopic techniques. The characterization analysis revealed that the rGO-BC surface was decorated with the MnFe2O4. The strong chemical interaction (via electrostatic and H-bonding) between the integrated surface of rGO-BC and MnFe2O4 nanoparticles has been reported. The prepared composite was highly porous with a heterogeneous surface. The average size of the prepared composite was reported in the ranges of 2.6–7.0 nm. The specific surface area of the prepared composite was calculated to be 50.3 m2/g with a pore volume of 0.061 cc/g and a half pore width of 8.4 Å. As well, many functional sites on the nanohybrid composite surface were also found. This results in the excellent adsorption properties of nanohybrid composite and the effectual elimination of methylene blue dye from water. The nanohybrid was tested for various linear isotherms, such as Langmuir and Freundlich, for the adsorption of methylene blue dye. The Freundlich isotherm was the well-fitted model, proving the adsorption is multilayer. The maximum Langmuir adsorption capacity of nanohybrid composite for methylene blue was reported to be 74.627 mg/g at 27 °C. The adsorption kinetics followed the pseudo-second-order recommended surface interaction between the dye and nanohybrid composite. The interaction between methylene blue and the nanohybrid composite was also confirmed from the FTIR spectrum of the methylene blue-loaded adsorbent. The rate-determining step for the present study was intraparticle diffusion. Temperature-dependent studies of methylene blue adsorption were also carried out to estimate adsorption’s free energy, enthalpy, and entropy. The methylene blue adsorption was feasible, spontaneous, and endothermic. A comparison study revealed that the present materials could be successfully prepared and used for wastewater treatment.
Finding the Right Solvent: A Novel Screening Protocol for Identifying Environmentally Friendly and Cost-Effective Options for Benzenesulfonamide
Authors: Cysewski Piotr , Jeliński Tomasz , Przybyłek Maciej
Published: June 2023

This study investigated the solubility of benzenesulfonamide (BSA) as a model compound using experimental and computational methods. New experimental solubility data were collected in the solvents DMSO, DMF, 4FM, and their binary mixtures with water. The predictive model was constructed based on the best-performing regression models trained on available experimental data, and their hyperparameters were optimized using a newly developed Python code. To evaluate the models, a novel scoring function was formulated, considering not only the accuracy but also the bias–variance tradeoff through a learning curve analysis. An ensemble approach was adopted by selecting the top-performing regression models for test and validation subsets. The obtained model accurately back-calculated the experimental data and was used to predict the solubility of BSA in 2067 potential solvents. The analysis of the entire solvent space focused on the identification of solvents with high solubility, a low environmental impact, and affordability, leading to a refined list of potential candidates that meet all three requirements. The proposed procedure has general applicability and can significantly improve the quality and speed of experimental solvent screening.
Research Progress of Hydrogen Production Technology and Related Catalysts by Electrolysis of Water
Authors: Li Haiyao , Guo Jun , Li Zhishan , Wang Jinsong
Published: June 2023

As a clean and renewable energy source for sustainable development, hydrogen energy has gained a lot of attention from the general public and researchers. Hydrogen production by electrolysis of water is the most important approach to producing hydrogen, and it is also the main way to realize carbon neutrality. In this paper, the main technologies of hydrogen production by electrolysis of water are discussed in detail; their characteristics, advantages, and disadvantages are analyzed; and the selection criteria and design criteria of catalysts are presented. The catalysts used in various hydrogen production technologies and their characteristics are emphatically expounded, aiming at optimizing the existing catalyst system and developing new high-performance, high-stability, and low-cost catalysts. Finally, the problems and solutions in the practical design of catalysts are discussed and explored.
Profiling of the Polyphenol Content of Honey from Different Geographical Origins in the United States
Authors: Nyarko Kate , Boozer Kaitlyn , Greenlief C. Michael
Published: June 2023

The presence of phenolic compounds in honey can serve as potential authenticity markers for honey’s botanical or geographical origins. The composition and properties of honey can vary greatly depending on the floral and geographical origins. This study focuses on identifying the specific markers that can distinguish honey based on their geographical areas in the United States. The main approach presented in this study to identify the geographic origins of honey involves chemometric methods combined with phenolic compound fingerprinting. Sample clean-up and phenolic compound extraction was carried out using solid phase extraction (SPE). Reversed phase liquid chromatography in combination with tandem mass spectrometry were utilized for the separation of the compounds. The honey physicochemical qualities were predominantly determined via spectrophotometric methods. Multivariate statistical tools such as principal component analysis (PCA), analysis of variance (ANOVA), and partial-least squares discriminant analysis (PLS-DA) were employed as both classification and feature selection tools. Overall, the present study was able to identify the presence of 12 potential markers to differentiate the honey’s geographical origins. The total phenolic content ranged from 81.6 to 105.7 mg GAE/100 g corresponding to honey from Colorado and Washington, respectively (GAE: gallic acid equivalents). The regression analysis shows a tendency for the total phenolic content of honey to increase as the color of honey increases. The most important result obtained in this study is the demonstration that the geographical origin of honey plays a critical role in predicting the physical properties and phenolic composition of honey.
Preparation and Characterization of Non-Crimping Laminated Textile Composites Reinforced with Electrospun Nanofibers
Authors: Sanchaniya Jaymin Vrajlal , Lasenko Inga , Kanukuntla Sai Pavan , Mannodi Anunand , Viluma-Gudmona Arta , Gobins Valters
Published: June 2023

This research investigated the use of electrospun nanofibers as reinforcing laminates in textiles to enhance their mechanical properties for use as smart and technical textile applications. Crimping plays a crucial role in textiles. Because of crimp, fabrics have extensibility, compressibility, and improved quality. Although crimping is inevitable for fabrics used in smart textiles, it is also a disadvantage as it could weaken the fibers and reduce their strength and efficiency. The study focused on preparing laminated textile composites by electrospinning a polyacrylonitrile (PAN) polymer onto textile fabric. The research examined the effect of electrospun nanofibers on the fabric by using a tensile testing machine and scanning electron microscopy. The results revealed that the prepared laminated textile was crimp-free because of the orientation of the nanofibers directly electrospun on the fabric, which exhibited perfect bonding between the laminates. Additionally, the nanofiber-reinforced composite fabrics demonstrated a 75.5% increase in the elastic moduli and a 20% increase in elongation at breaking. The study concluded that the use of electrospun nanofibers as laminates in textile composites could enhance the elastic properties, and prepared laminated composites will have the advantages of nanofibers, such as crimp-free elastic regions. Furthermore, the mechanical properties of the laminated textile composite were compared with those of the micromechanical models, providing a deeper understanding of the behavior of these laminated composites.
Nutritional Care for Older Patients Undergoing Rehabilitation
Authors: Morita Kae , Shirai Yuka , Tohyama Momoko , Momosaki Ryo
Published: June 2023

Malnutrition is a common complication in patients undergoing rehabilitation [...]
Assessment of Lifestyle, Eating Habits and the Effect of Nutritional Education among Undergraduate Students in Southern Italy
Authors: Moscatelli Fiorenzo , De Maria Antonella , Marinaccio Luigi Antonio , Monda Vincenzo , Messina Antonietta , Monacis Domenico , Toto Giusi , Limone Pierpaolo , Monda Marcellino , Messina Giovanni , Monda Antonietta , Polito Rita
Published: June 2023

Background: The years spent at university represent a critical period that can influence both the quality of lifestyle and the eating habits of subsequent adulthood, and also, in the long term, the health of the individual. The aim of this study was to investigate the lifestyle of university students living away from home. Methods: Each subject recruited for the study was given a questionnaire to obtain general information, eating habits and physical activity levels before (T0) and after six month of training seminars (T1). Blood pressure, body composition and questionnaire responses were investigated. Results: The main findings of this study are a significant decrement in blood pressure; an increment in physical activity practice; an increased number of subjects who pay attention to the calorific value of food and also an improvement in BIA parameters. Conclusions: In conclusion, this study demonstrated the challenges that university students face in leading a healthy lifestyle and caring for their nutritional needs, particularly when they are away from their families. No intervention specifically targets young adults, even though much emphasis is placed on the promotion of a healthy lifestyle based on a varied and balanced diet and sufficient exercise. Our study showed that it is possible to improve lifestyle through educational events aimed at making students aware of the health risks deriving from unhealthy lifestyles.
Legume Consumption and Blood Pressure Control in Individuals with Type 2 Diabetes and Hypertension: Cross-Sectional Findings from the TOSCA.IT Study
Authors: Vitale Marilena , Giosuè Annalisa , Sieri Sabina , Krogh Vittorio , Massimino Elena , Rivellese Angela Albarosa , Riccardi Gabriele , Vaccaro Olga , Masulli Maria
Published: June 2023

Background: Our aims were to evaluate the relationship of habitual legume consumption with blood pressure (BP) control in a large cohort of people with T2D and hypertension, and to investigate whether specific nutritional components of legumes or other foods may contribute to regulate BP levels. Methods: We studied 1897 participants with T2D and hypertension. Dietary habits were assessed through a validated food frequency questionnaire. Sex-specific quartiles of legume consumption were created. Results: Higher legume consumption was associated with a lower intake of energy, carbohydrates, glycaemic load, alcohol, and sodium, and a significantly greater intake of proteins, fat, monounsaturated, polyunsaturated, fibre, potassium, and polyphenols. Significantly lower systolic and diastolic BP values were observed in the highest vs. lowest quartile of legume consumption (132.9 ± 6.7 vs. 137.3 ± 7.0 mmHg, p < 0.001; 78.9 ± 4.1 vs. 81.0 ± 4.2 mmHg, p = 0.002; respectively), as well as the proportion of people meeting the treatment targets (61.3% vs. 37.4% and 71.3% vs. 52.4%, respectively, p < 0.01). This association was independent from other foods whose consumption is associated with the high legume intake. Conclusions: In people with T2D and hypertension, three servings of legumes per week are associated with significantly better BP control. This gives further support to current dietary guidelines in recommending the frequent consumption of legumes, as a “ready-to-use” dietary strategy to achieve optimal BP control.
Oxygen in the Earth System
Authors: Hocke Klemens
Published: June 2023

Atmospheric oxygen is produced and consumed by life on Earth, and the ozone layer protects life on Earth from harmful solar UV radiation. The research on oxygen in the Earth system is of interest to many different geoscientific communities, from paleoclimatology to aeronomy. I provide a brief overview of the research activities and their motivations. In situ measurements and remote sensing of atmospheric oxygen are described. The global evolution, distribution, and trends of atmospheric oxygen are discussed.
Functional Granulocyte–Macrophage Colony-Stimulating Factor (GM-CSF) Delivered by Canine Histiocytic Sarcoma Cells Persistently Infected with Engineered Attenuated Canine Distemper Virus
Authors: Marek Katarzyna , Armando Federico , Asawapattanakul Thanaporn , Nippold Vanessa Maria , Plattet Philippe , Gerold Gisa , Baumgärtner Wolfgang , Puff Christina
Published: June 2023

The immune response plays a key role in the treatment of malignant tumors. One important molecule promoting humoral and cellular immunity is granulocyte–macrophage colony-stimulating factor (GM-CSF). Numerous successful trials have led to the approval of this immune-stimulating molecule for cancer therapy. However, besides immune stimulation, GM-CSF may also accelerate tumor cell proliferation, rendering this molecule a double-edged sword in cancer treatment. Therefore, detailed knowledge about the in vitro function of GM-CSF produced by infected tumor cells is urgently needed prior to investigations in an in vivo model. The aim of the present study was to functionally characterize a persistent infection of canine histiocytic sarcoma cells (DH82 cells) with the canine distemper virus strain Onderstepoort genetically engineered to express canine GM-CSF (CDV-Ondneon-GM-CSF). The investigations aimed (1) to prove the overall functionality of the virally induced production of GM-CSF and (2) to determine the effect of GM-CSF on the proliferation and motility of canine HS cells. Infected cells consistently produced high amounts of active, pH-stable GM-CSF, as demonstrated by increased proliferation of HeLa cells. By contrast, DH82 cells lacked increased proliferation and motility. The significantly increased secretion of GM-CSF by persistently CDV-Ondneon-GM-CSF-infected DH82 cells, the pH stability of this protein, and the lack of detrimental effects on DH82 cells renders this virus strain an interesting candidate for future studies aiming to enhance the oncolytic properties of CDV for the treatment of canine histiocytic sarcomas.
An In Silico Study Investigating Camptothecin-Analog Interaction with Human Protein Tyrosine Phosphatase, SHP2 (PTPN11)
Authors: Bajia Donald , Derwich Katarzyna
Published: June 2023

The human PTPN11 gene encodes for the src tyrosine phosphatase protein (SHP2) is now gaining much attention in many disorders, particularly its oncogenic involvement in many types of cancer. Efforts in developing molecules targeting SHP2 with high efficacy are the future of drug discovery and chemotherapy. However, the interaction of a new camptothecin analog with the catalytic domain of SHP2 protein remains unknown. Therefore, this study aims to provide in silico rationale for the recognition and binding of FL118 and irinotecan with the catalytic domain of human protein tyrosine phosphatase-SHP2 (PTPc-SH2-SHP2, chain A). The docking interaction of the human SHP2 protein’s catalytic domain as well as Y279C and R465G mutants with FL118 and irinotecan ligands were calculated and analyzed using the Autodock 4.2 programme, setting the docking grid to target the protein’s active site. The camptothecin analog FL118 had the best lowest negative affinity energies with PTPc-SHP2 wildtype and SHP2-Y279C mutant model (−7.54 Kcal/mol and −6.94 Kcal/mol, respectively). Moreover, the protein-ligand complexes revealed several hydrogen bond interactions reflecting the degree of stability that each structure possesses, with the FL118-SHP2-wildtype forming the most stable complex among the structures. In addition, the FL118-SHP2 wildtype complex was validated for RMSD, RMSF, hydrogen bonds, and salt bridges. This revealed that the complex generated became stable over time. This in silico rationale identifies the novel FL118 camptothecin analog as a potent selective inhibitor of PTPc-SH2 domain of SHP2 protein, paving way for further in vitro investigations into the interactions and binding activity of analogs with SHP2 for potential therapeutic applications in PTPN11-associated disorders.
Cyclic RGD-Functionalized pH/ROS Dual-Responsive Nanoparticle for Targeted Breast Cancer Therapy
Authors: Yao Pu , Wang Xiaowen , Wang Qianmei , Dai Qing , Peng Yu , Yuan Qian , Mou Nan , Lv Shan , Weng Bangbi , Wang Yu , Sun Fengjun
Published: June 2023

Breast cancer is the most common malignant tumor in women and is a big challenge to clinical treatment due to the high morbidity and mortality. The pH/ROS dual-responsive nanoplatforms may be an effective way to significantly improve the therapeutic efficacy of breast cancer. Herein, we report a docetaxel (DTX)-loaded pH/ROS-responsive NP that could achieve active targeting of cancer cells and selective and complete drug release for effective drug delivery. The pH/ROS-responsive NPs were fabricated using nanocarriers that consist of an ROS-responsive moiety (4-hydroxymethylphenylboronic acid pinacol ester, HPAP), cinnamaldehyde (CA, an aldehyde organic compound with anticancer activities) and cyclodextrin (α-CD). The NPs were loaded with DTX, modified with a tumor-penetration peptide (circular RGD, cRGD) and named DTX/RGD NPs. The cRGD could promote DTX/RGD NPs penetration into deep tumor tissue and specifically target cancer cells. After internalization by cancer cells through receptor-mediated endocytosis, the pH-responsive acetal was cleaved to release CA in the lysosomal acidic environment. Meanwhile, the high ROS in tumor cells induced the disassembly of NPs with complete release of DTX. In vitro cellular assays verified that DTX/RGD NPs could be effectively internalized by 4T1 cells, obviously inducing apoptosis, blocking the cell cycle of 4T1 cells and consequently, killing tumor cells. In vivo animal experiments demonstrated that the NPs could target to the tumor sites and significantly inhibit the tumor growth in 4T1 breast cancer mice. Both in vitro and in vivo investigations demonstrated that DTX/RGD NPs could significantly improve the antitumor effect compared to free DTX. Thus, the DTX/RGD NPs provide a promising strategy for enhancing drug delivery and cancer therapy.
More Than Pigments: The Potential of Astaxanthin and Bacterioruberin-Based Nanomedicines
Authors: Morilla Maria Jose , Ghosal Kajal , Romero Eder Lilia
Published: June 2023

Carotenoids are natural products regulated by the food sector, currently used as feed dyes and as antioxidants in dietary supplements and composing functional foods for human consumption. Of the nearly one thousand carotenoids described to date, only retinoids, derived from beta carotene, have the status of a drug and are regulated by the pharmaceutical sector. In this review, we address a novel field: the transformation of xanthophylls, particularly the highly marketed astaxanthin and the practically unknown bacterioruberin, in therapeutic agents by altering their pharmacokinetics, biodistribution, and pharmacodynamics through their formulation as nanomedicines. The antioxidant activity of xanthophylls is mediated by routes different from those of the classical oral anti-inflammatory drugs such as corticosteroids and non-steroidal anti-inflammatory drugs (NSAIDs): remarkably, xanthophylls lack therapeutic activity but also lack toxicity. Formulated as nanomedicines, xanthophylls gain therapeutic activity by mechanisms other than increased bioavailability. Loaded into ad hoc tailored nanoparticles to protect their structure throughout storage and during gastrointestinal transit or skin penetration, xanthophylls can be targeted and delivered to selected inflamed cell groups, achieving a massive intracellular concentration after endocytosis of small doses of formulation. Most first reports showing the activities of oral and topical anti-inflammatory xanthophyll-based nanomedicines against chronic diseases such as inflammatory bowel disease, psoriasis, atopic dermatitis, and dry eye disease emerged between 2020 and 2023. Here we discuss in detail their preclinical performance, mostly targeted vesicular and polymeric nanoparticles, on cellular models and in vivo. The results, although preliminary, are auspicious enough to speculate upon their potential use for oral or topical administration in the treatment of chronic inflammatory diseases.
Refractive Properties of Conjugated Organic Materials Doped with Fullerenes and Other Carbon-Based Nano-Objects
Authors: Kamanina Natalia
Published: June 2023

Due to the high demand for optoelectronics for use in new materials and processes, as well as the search for their modeling properties, the expansion of the functionality of modified materials using nanotechnology methods is relevant and timely. In the current paper, a specific nanotechnology approach is shown to increase the refractive and photoconductive parameters of the organic conjugated materials. The sensitization process, along with laser treatment, are presented in order to improve the basic physical–chemical properties of laser, solar energy, and general photonics materials. Effective nanoparticles, such as fullerenes, shungites, reduced graphene oxides, carbon nanotubes, etc., are used in order to obtain the bathochromic shift, increase the laser-induced change in the refractive index, and amplify the charge carrier mobility of the model matrix organics sensitized with these nanoparticles. The four-wave mixing technique is applied to test the main refractive characteristics of the studied materials. Volt–current measurements are used to estimate the increased charge carrier mobility. The areas of application for the modified nanostructured plastic matrixes are discussed and extended, while also taking into account the surface relief.
Vibration of a Liquid Crystal Elastomer Spring Oscillator under Periodic Electrothermal Drive
Authors: Li Kai , Lou Jiangfeng , Hu Shaofei , Dai Yuntong , Wang Fei , Yu Yong
Published: June 2023

The oscillations of electrically actuated thermally-responsive liquid crystal elastomer (LCE) microfibers under cyclic electric actuation have been discovered in recent experiments. Periodic electric actuation is a common method of active control with potential applications in the fields of micro-actuators. In this paper, the vibration behavior of LCE spring oscillator under periodic electrothermal drive is studied theoretically. Based on the dynamic LCE model, the dynamic governing equation of the LCE spring oscillator is established, and the time history curves of the vibration are obtained by numerical calculations. The results show that the periodic electrothermal drive can cause periodic vibration of the LCE spring oscillator. With the increase of time rate, the vibration amplitude increases first and then decreases. In a small damping system, there exist optimal sets of electrothermal drive period and electrothermal drive time rate to maximize the system amplitude. For the optimum periodic mode, the vibration amplitude of the spring oscillator is affected by the current heat, damping coefficient, gravital acceleration, spring constant and shrinkage coefficient, but not by the initial velocity. The application examples of LCE materials show that periodic electrothermally driven LCEs have promising applications. The results of this study are instructive for the design of soft robots and LCE-based electric locomotives.
Three-Dimensionally Printed Hydrogel Cardiac Patch for Infarct Regeneration Based on Natural Polysaccharides
Authors: Loureiro Jorge , Miguel Sónia P. , Galván-Chacón Victor , Patrocinio David , Pagador José Blas , Sánchez-Margallo Francisco M. , Ribeiro Maximiano P. , Coutinho Paula
Published: June 2023

Myocardial infarction is one of the more common cardiovascular diseases, and remains the leading cause of death, globally. Hydrogels (namely, those using natural polymers) provide a reliable tool for regenerative medicine and have become a promising option for cardiac tissue regeneration due to their hydrophilic character and their structural similarity to the extracellular matrix. Herein, a functional ink based on the natural polysaccharides Gellan gum and Konjac glucomannan has, for the first time, been applied in the production of a 3D printed hydrogel with therapeutic potential, with the goal of being locally implanted in the infarcted area of the heart. Overall, results revealed the excellent printability of the bioink for the development of a stable, porous, biocompatible, and bioactive 3D hydrogel, combining the specific advantages of Gellan gum and Konjac glucomannan with proper mechanical properties, which supports the simplification of the implantation process. In addition, the structure have positive effects on endothelial cells’ proliferation and migration that can promote the repair of injured cardiac tissue. The results presented will pave the way for simple, low-cost, and efficient cardiac tissue regeneration using a 3D printed hydrogel cardiac patch with potential for clinical application for myocardial infarction treatment in the near future.
What Is Wrong with Hyaluronic Acid Chemistry? A 15N/13C Solid-State NMR Re-Evaluation of Its Dopamine Conjugates
Authors: Grosu Ioana-Georgeta , Moț Augustin , Filip Xenia , Filip Claudiu
Published: June 2023

In this work, a systematic 15N/13C solid-state NMR investigation is performed on three dopamine (DA) conjugates of hyaluronic acid, considered in both its native (HA) and NaIO4-oxidized (HAOx) forms. Two of them, here named HAEDC-DA and HAOx-DA, have been previously introduced as covalent conjugates involving DA amine nitrogen: the former by EDC-mediated amide bond formation, and the latter by reaction of the Schiff base with the aldehyde moieties presumed to exist in HAOx. The third conjugate, HA-DA, is reported here for the first time; it is obtained by simply mixing hyaluronan with DA∙HCl at pH 5. The 15N ss-NMR spectra were found to be consistent in all the systems, and the DA molecules were found to be in their charged -NH3+ form, which contradicts the HAEDC-DA/HAOx-DA covalent bonding schemes proposed in the literature. The 13C ss-NMR results add useful new insights into the structure and interaction patterns of the conjugates. All of our findings are relevant for future practical applications, for instance in developing novel HA-based hydrogels. In addition, the present study demonstrates the importance of using the most appropriate analytical tools when investigating composite systems due to the complexity of hyaluronic acid conjugates. Solid-state NMR proved essential to answering the question in the title: actually, there is nothing wrong with hyaluronic acid chemistry; the claimed covalent bonds between DA and the HA(HAOx) chain do not exist in these systems, because the conditions for their formation do not hold in practice.
Changes in Vegetation Resistance and Resilience under Different Drought Disturbances Based on NDVI and SPEI Time Series Data in Jilin Province, China
Authors: Ma Jiani , Zhang Chao , Li Shaner , Yang Cuicui , Chen Chang , Yun Wenju
Published: June 2023

Extreme drought is increasing in frequency and intensity in many regions globally. Understanding the changes in vegetation resistance and resilience under aggravated drought is essential for maintaining regional ecosystem stability. In this study, a drought event–vegetation response framework was developed to explore vegetation resistance and resilience changes. The normalized difference vegetation index (NDVI) was correlated with the standardized precipitation evapotranspiration index (SPEI) at multiple timescales to screen out the vegetation response time to drought. Then, the SPEI for the response time was detected using run theory to identify drought events during the period 2000–2017. Finally, drought-induced NDVI anomaly changes were identified using a sliding window to explore the changes in resistance and resilience to drought. This study focuses on Jilin province, China, which contains a famous environmentally vulnerable area. The results illustrate that the response time of vegetation to drought is 3 months. The northwest of Jilin province is considered to be drought-vulnerable because it has suffered the most drought events, i.e., 19–21 times, with severities in the range of 2.6–3.2 and durations in the range of 3.6–4.1 months. Grassland shows the weakest resistance and the strongest resilience, and tree cover shows the strongest resistance and the weakest resilience under severe drought disturbance among all vegetation. As the severity and duration of drought increase, the resistance decreases, and the resilience increases. During the growing season, the drought from May to July significantly impacts the vegetation resistance. Drought occurring from June to July has much less impact on resilience. Drought in August to September has less impact on resistance and a more significant impact on resilience. The results of this study may increase our knowledge regarding the response of vegetation to drought and guide ecosystem stability restoration.
Performance Degradation Assessment of Railway Axle Box Bearing Based on Combination of Denoising Features and Time Series Information
Authors: Liu Zhigang , Zhang Long , Xiao Qian , Huang Hao , Xiong Guoliang
Published: June 2023

In the existing rolling bearing performance degradation assessment methods, the input signal is usually mixed with a large amount of noise and is easily disturbed by the transfer path. The time information is usually ignored when the model processes the input signal, which affects the effect of bearing performance degradation assessment. To solve the above problems, an end-to-end performance degradation assessment model of railway axle box bearing based on a deep residual shrinkage network and a deep long short-term memory network (DRSN-LSTM) is proposed. The proposed model uses DRSN to extract local abstract features from the signal and denoises the signal to obtain the denoised feature vector, then uses deep LSTM to extract the time-series information of the signal. The healthy time-series signal of the rolling bearing is input into the DRSN-LSTM reconstruction model for training. Time-domain, frequency-domain, and time–frequency-domain features are extracted from the signal both before and after reconstruction to form a multi-domain features vector. The mean square error of the two feature vectors is used as the degradation indicator to implement the performance degradation assessment. Artificially induced defects and rolling bearings life accelerated fatigue test data verify that the proposed model is more sensitive to early failures than mathematical models, shallow networks or other deep learning models. The result is similar to the development trend of bearing failures.
An Improved YOLOv7 Lightweight Detection Algorithm for Obscured Pedestrians
Authors: Li Chang , Wang Yiding , Liu Xiaoming
Published: June 2023

The detection algorithm commonly misses obscured pedestrians in traffic scenes with a high pedestrian density because mutual occlusion among pedestrians reduces the prediction box score of the concealed pedestrians. The paper uses the YOLOv7 algorithm as the baseline and makes the following three improvements by investigating the variables influencing the detection method’s performance: First, the backbone network of the YOLOv7 algorithm is replaced with the lightweight feature extraction network Mobilenetv3 since the pedestrian detection algorithm frequently needs to be deployed in driverless mobile, which requires a fast operating speed of the algorithm; second, a high-resolution feature pyramid structure is suggested for the issue of missed detection of hidden pedestrians, which upscales the feature maps generated from the feature pyramid to increase the resolution of the output feature maps and introduces shallow feature maps to strengthen the distinctions between adjacent sub-features to enhance the network’s ability to extract features for the visible area of hidden pedestrians and small-sized pedestrians in order to produce deeper features with greater differentiation for pedestrians; and the third is to suggest a detection head based on an attention mechanism that is employed to lower the confidence level of target neighboring sub-features, lower the quantity of redundant detection boxes, and lower the following NMS computation. The mAP of the suggested approach in this work achieves 89.75%, which is 9.5 percentage points better than the YOLOv7 detection algorithm, according to experiments on the CrowdHuman pedestrian-intensive dataset. The algorithm proposed in this paper can considerably increase the detection performance of the detection algorithm, particularly for obscured pedestrians and small-sized pedestrians in the dataset, according to the experimental effect plots.