bluuebunny/crossref_metadata_2025 huggingface dataset:
Will Multidisciplinary Collaboration Reduce the Disability Rate of Diabetic Foot (2009–2019)?—A Study Based on the Perspective of Organizational Reform
Authors: Hou Mengchi , Gong Xue , Chang Wenhu , Dong Jie , Zhao Feifei , Ji Zhili , Guo Rui
Published: October 2021

Objectives: Discuss the experience and practice of multidisciplinary cooperation of diabetic foot in China and analyze its impact on the quality of care.

Methods: This study observed the medical procedure by interviewing 12 key personnel in-depth. We extracted data from medical records and assessed the effect of MDT in three dimensions: quality, efficiency, and cost, to eventually achieve a final conclusion.

Results: The studied reform includes the following three aspects: the adjustment of hospital buildings layout and disciplines, one-stop outpatient, and one-stop inpatient service. After the multidisciplinary collaboration, the rate of above-knee amputation is reduced by 3.63%, the disability score per 100 diabetic foot patients decreases by 6.12, the average length of stay decreases significantly, and the cost of hospitalization shows an increasing trend.

Conclusions: Multidisciplinary collaboration is performed based on spatial layout adjustment and clinical pathway optimization, which provide more comprehensive and integrated care than a general medical team or a single specialist, thereby reducing the rate of disability, shortening the length of hospitalization. Besides, the new measurable indicator called disability score per 100 diabetic foot patients has been verified to evaluate the living ability of patients after surgery. This paper provides a reference for organizational reform of multidisciplinary diseases to support treatment and management of other multiorgan diseases.
Early Classification of Intent for Maritime Domains Using Multinomial Hidden Markov Models
Authors: Carlson Logan , Navalta Dalton , Nicolescu Monica , Nicolescu Mircea , Woodward Gail
Published: October 2021

The need for increased maritime security has prompted research focus on intent recognition solutions for the naval domain. We consider the problem of early classification of the hostile behavior of agents in a dynamic maritime domain and propose our solution using multinomial hidden Markov models (HMMs). Our contribution stems from a novel encoding of observable symbols as the rate of change (instead of static values) for parameters relevant to the task, which enables the early classification of hostile behaviors, well before the behavior has been finalized. We discuss our implementation of a one-versus-all intent classifier using multinomial HMMs and present the performance of our system for three types of hostile behaviors (ram, herd, block) and a benign behavior.
In vitro Characterization of Biofilm Formation in Prevotella Species
Authors: Albaghdadi Shurooq Zakariya , Altaher Jenan Bader , Drobiova Hana , Bhardwaj Radhika G. , Karched Maribasappa
Published: October 2021

Background: Periodontitis, a chronic inflammatory oral infection is the outcome of disturbances in the homeostasis of the oral biofilm microbiota. A number of studies have found the occurrence of Prevotella species in elevated levels in periodontitis compared to healthy subjects. Even though different aspects of Prevotella as part of oral biofilm have been studied, in vitro biofilms formed by these species have not been characterized systematically. The objective of this study was to characterize biofilms formed by several Prevotella species and further to assess biofilm inhibition and detachment of preformed biofilms.

Methods: Biofilms were grown in 24-well plates containing brucella broth in anaerobic conditions for 3 days, and were quantified using crystal violet staining. Images of SYTO 9 Green fluorescent stained biofilms were captured using confocal microscopy. Biofilm inhibition and detachment by proteinase and DNase I was tested. The biochemical characterization included quantification of proteins and DNA in the biofilms and biofilm-supernatants.

Results:Prevotella loescheii, Prevotella oralis and Prevotella nigrescens showed highest biofilm formation. P. nigrescens formed significantly higher amounts of biofilms than P. loescheii (P = 0.005) and P. oralis (P = 0.0013). Inhibition of biofilm formation was significant only in the case of P. oralis when treated with proteinase (P = 0.037), whereas with DNase I treatment, the inhibition was not significant (P = 0.531). Overall, proteinase was more effective in biofilm detachment than DNase I. Protein and DNA content were higher in biofilm than the supernatant with the highest amounts found in P. nigrescens biofilm and supernatants. P. oralis biofilms appeared to secrete large amounts of proteins extracellularly into the biofilm-supernatants.

Conclusion: Significant differences among Prevotella species to form biofilms may imply their variable abilities to get integrated into oral biofilm communities. Of the species that were able to grow as biofilms, DNase I and proteinase inhibited the biofilm growth or were able to cause biofilm detachment.
Growing Spatial Overlap Between Dam-Related Flooding, Cropland and Domestic Water Points: A Water–Energy–Food Nexus Management Challenge in Malawi and Ghana
Authors: Li Chengxiu , Yu Weiyu , Dzodzomenyo Mawuli , Asamoah Moses , Kerapetse Catherine Tlotlo , Kandel Matt , Wright Jim
Published: October 2021

In sub-Saharan Africa, land cover change, expansion of hydropower infrastructure, and increased flooding complicate country-level efforts to meet the Sustainable Development Goal target concerning access to safe water. The Water, Energy and Food (WEF) nexus approach recognises that addressing these complex challenges requires cross-sectoral analyses at multiple scales. Building on such an approach, our study examined the interrelationships between land cover change, dam-related flooding and access to safe water via a national-level spatial analysis with local case studies in Malawi and Ghana. Our assessment of the water–food interactions found that areas of overlap between water points and cropland increased from 2000 to 2020 for both countries at national scale, but overlap extent varied greatly depending on the land cover product used. Local-scale exploration of water point installation patterns in Zomba, Malawi confirmed this pattern, highlighting increasing non-governmental funding of borehole installation programmes. Our assessment of water–energy interactions found that flooding mediated by hydropower dams increased for the White Volta Basin in Ghana, thereby increasing inundation of groundwater points. Local-scale focus group discussions revealed flooding resulted in contaminated water sources and high risk of injury or drowning whilst fetching water. Overall, our study highlights how socio-economic drivers are bringing water points, flooding and cropland into closer proximity, requiring flood mitigation measures at water points and agro-chemical management to minimise potential water quality impacts. Given differences between land cover products, we recommend more robust integration of existing land cover products to better monitor these phenomena.
Research on Labor Market Institutional Environment and Labor Agglomeration—Analysis Based on Dynamic Spatial Panel Data
Authors: Guan Huaping , Zhang Jianwu , Zhu Xiaoping
Published: October 2021

The institutional environment not only has a great impact on economic development but also has a certain impact on labor agglomeration. This paper explains the phenomenon of labor agglomeration from the perspective of the labor market institutional environment. Taking the labor market institutional environment including social contract environment and labor contract environment as the premise, it is considered that a good labor institutional market can promote labor agglomeration and technological progress can stimulate the agglomeration of highly-skilled talents through theoretical analysis. Based on the provincial panel data from 2001 to 2017, the empirical analysis is conducted to verify the relevant factors through the dynamic spatial Durbin model. The paper finally puts forward some policy suggestions to maintain a good institutional environment of the labor market, including that the local government should continue to improve the labor dispute settlement system, strengthen the protection of labor rights and interests, and enhance the institutional environment of the regional labor market.
Biocultural Diversity for Food System Transformation Under Global Environmental Change
Authors: Argumedo Alejandro , Song Yiching , Khoury Colin K. , Hunter Danny , Dempewolf Hannes , Guarino Luigi , de Haan Stef
Published: October 2021

Biocultural diversity is central to the nutrition, resilience, and adaptive capacity of Indigenous and traditional peoples, who collectively maintain the longest ongoing human experiences with the provision of food under environmental change. In the form of crops and livestock and associated knowledge on their cultivation and use, food-related biocultural diversity likewise underpins global food security. As food system transformation is increasingly recognized as an urgent priority, we argue that food security, sustainability, resilience, and adaptive capacity can be furthered through greater emphasis on conservation, use, and celebration of food-related biocultural diversity. We provide examples from the Parque de la Papa, Peru, a “food biocultural diversity neighborhood” which through advocacy and partnerships based around its diversity, has both enhanced local communities and contributed to food security at a much larger scale. We outline collaborative actions which we believe are important to up- and out-scale food biocultural diversity neighborhood successes. Further research and knowledge sharing are critical to better document, understand, track, and communicate the value, functions, and state of biocultural diversity in food systems. Expanded training and capacity development opportunities are important to enable the interchange of experiences and visions on food, health, sustainability and resilience, climate adaptation, equity and justice, and livelihood generation with others facing similar challenges. Finally, strengthened networking across food biocultural diversity neighborhoods is essential to their persistence and growth as they increasingly engage with local, national, and international organizations, based on shared interests and on their own terms, across five continents.
Developing a Method to Precisely Locate the Keypoint During Craniotomy Using the Retrosigmoid Keyhole Approach: Surgical Anatomy and Technical Nuances
Authors: Jian Zhi-heng , Sheng Min-feng , Li Jia-yan , An De-zhu , Weng Zhi-jian , Chen Gang
Published: October 2021

Objective: To explore the precise location of the keypoint during craniotomy using the retrosigmoid keyhole approach.

Methods: This study included 20 dry skulls and 10 wet cadaveric specimens. On the inner surface of dry skulls, the junction between the inferior margin of the transverse sinus (ITS) and the posterior margin of the sigmoid sinus (TSJ) was marked. The keypoint (D) was identified as the TSJ's corresponding point on the external surface of the temporal mastoid process (MP). The distance from the keypoint to the top point of the digastric groove, mastoidale, and asterion were noted (AD, BD, CD, respectively). A method to accurately locate the keypoint was developed based on these relationships. The developed method was used on the wet cadaveric specimens to evaluate its accuracy, safety, rapidity, and minimal invasion.

Results: No significant difference was found between the AD, BD, and CD of the left and right sides. The drilling point was oriented on a straight line 12 mm above the top point of digastric groove, perpendicular to the Frankfort horizontal plane (FHP). In the cadaveric specimens, the operative area was clearly exposed. No venous sinus rupture occurred. The average craniotomy time was 28.74 ± 3.89 min.

Conclusions: A potentially safe, accurate, and rapid craniotomy procedure was developed with the added advantage of preserving the visibility of the operating field and preventing venous sinus injury.
Rare Metastasis of Gastric Cancer to the Thyroid Gland: A Case Report and Review of Literature
Authors: Wu Zehui , Guo Tao , Li Qiang , Cheng Liang , Hu Xiaosi , Xu Aman
Published: October 2021

Background: It is common for patients with gastric cancer to develop distant metastases in the liver, lung, bone, and brain. Although the thyroid also has an abundant blood supply, gastric cancer metastasis to the thyroid is uncommon. Due to the rarity of such metastasis, its clinical features are not well understood. Here, we present the case of a patient with gastric cancer metastasis to the thyroid treated at our hospital.

Case Summary: We report the case of a 63-year-old female with a mass in the anterior neck and mild hoarseness for 6 months. The patient underwent proximal subtotal gastrectomy for Siewert III oesophagogastric junction cancer 6 years ago. Subsequently, she received 8 cycles of adjuvant chemotherapy. Her condition was stable until mild hoarseness developed for no apparent reason 6 months prior to presenting at our clinic. Both ultrasonography and computed tomography confirmed a heterogeneous mass in the right lobe of the thyroid gland. Blood thyroid function tests and tumor marker expression levels were normal. Thyroid malignancy was suspected, and the patient underwent a right thyroidectomy. During the surgery, a tumor was found that had invaded the right recurrent laryngeal nerve and trachea. H&E staining and immunohistochemistry results suggested that the cancer cells originated from gastric cancer. The patient was diagnosed with thyroid metastasis of gastric cancer. She refused further treatment and died within 6 months.

Conclusion: Metastasis of gastric cancer to the thyroid is rare and is associated with a poor prognosis. Immunohistochemical diagnosis is essential for a conclusive diagnosis. For patients with a history of malignant tumors, the possibility of metastatic thyroid nodules should be ruled out when diagnosing thyroid nodules.
Commensal Rodent Habitat Expansion Enhances Arthropod Disease Vectors on a Tropical Volcanic Island
Authors: Wu De-Lun , Shih Han-Chun , Wang Jen-Kai , Teng Hwa-Jen , Kuo Chi-Chien
Published: October 2021

On volcanic islands, the release of animals from predators and competitors can lead to increased body size and population density as well as the expanded habitat use of introduced animals relative to their mainland counterparts. Such alterations might facilitate the spread of diseases on islands when these exotic animals also carry pathogenic agents; however, this has rarely been investigated. The commensal Asian house rat (Rattus tanezumi) is confined to human residential surroundings in mainland Taiwan but can be observed in the forests of nearby Orchid Island, which is a tropical volcanic island. Orchid Island is also a hot spot for scrub typhus, a lethal febrile disease transmitted by larval trombiculid mites (chiggers) that are infected primarily with the rickettsiaOrientia tsutsugamushi(OT). We predicted an increase in chigger abundance when rodents (the primary host of chiggers) invade forests from human settlements since soils are largely absent in the latter habitat but necessary for the survival of nymphal and adult mites. A trimonthly rodent survey at 10 sites in three habitats (human residential, grassland, and forest) found onlyR. tanezumiand showed moreR. tanezumiand chiggers in forests than in human residential sites. There was a positive association between rodent and chigger abundance, as well as between rodent body weight and chigger load. Lastly, >95% of chiggers wereLeptotrombidium delienseand their OT infection rates were similar among all habitats. Our study demonstrated potentially elevated risks of scrub typhus when this commensal rat species is allowed to invade natural habitats on islands. Additionally, while the success of invasive species can be ascribed to their parasites being left behind, island invaders might instead obtain more parasites if the parasite requires only a single host (e.g., trombiculid mite), is a host generalist (e.g.,L. deliense), and is transferred from unsuitable to suitable habitats (i.e., human settlements on the mainland to forests on an island).
Effects of Stem Cell Factor/c-Kit Signaling on In Vitro Maturation of Porcine Oocytes and Subsequent Developmental Competence After Fertilization
Authors: Kim Eunhye , Cai Lian , Hyun Sang-Hwan
Published: October 2021

Stem cell factor (SCF), also known as c-Kit ligand, plays an important role in the proliferation of primordial germ cells and the survival of oocytes during follicular development. The aim of this study was to investigate the effect of SCF/c-Kit signaling on in vitro maturation (IVM) of porcine oocytes by analyzing nuclear and cytoplasmic maturation, oocyte size, cumulus cell expansion, and developmental competence to the blastocyst stage. Moreover, mRNA expression patterns of porcine cumulus cells and oocytes were evaluated using qRT-PCR. Following 42 h of IVM, 10 and 50 ng/mL SCF-treated groups exhibited significantly (P < 0.05) increased polar body extrusion rates and intracellular glutathione levels compared with the control group. The cumulus expansion index significantly (P < 0.05) increased in all SCF-treated groups compared with the control samples. mRNA levels of the proapoptotic gene Bax and apoptosis-related cysteine peptidase Caspase3 were lower in SCF-treated cumulus cells than in the control group. Notably, the diameter of oocytes after IVM, the mRNA expression of well-known oocyte-secreted factors (GDF9 and BMP15), and an oocyte-specific protein essential for ovulation and oocyte health (YBX2) were significantly (P < 0.05) higher in SCF-treated than in non-treated oocytes. Inhibition of c-Kit during porcine IVM using ACK2, an antagonistic blocker of c-Kit, significantly (P < 0.05) decreased the polar body extrusion rate compared with the control, as well as blastocyst formation rate compared with the 10 ng/mL SCF-treated group. In conclusion, the effect of SCF/c-Kit-mediated signaling during porcine IVM could be ascribed to the reduced expression of apoptosis-related genes and higher expression of oocyte-specific/secreted factors.
The Role of European Schools and University Departments of Public Health in the 2020 COVID-19 Response, European Region, 2020
Authors: Bauernfeind Ariane , Foldspang Anders , Fernandez-Ajuria Alberto , Otok Robert , Middleton John
Published: October 2021

Objective: The main objective was to examine, how European Schools of Public Health (SPHs) responded to the COVID-19 pandemic through 2020, across the main activity domains of the SPHs.

Methods: A cross-sectional survey based on an online questionnaire concerning the anti-COVID-19 activities from 1st March to 31st October 2020 of the 117 members of the Association of Schools of Public Health in the European Region (ASPHER). The questionnaire asked about 33 sub-themes within the four main themes of teaching, health communication to the public, research, and consultancy/advice.

Results: Fifty-nine SPHs (50%) completed the questionnaire. Seventy-nine per cent of participants were involved in COVID-19 related teaching; health communication to the public, 76%; research, 80%; consultancy/advice, 81%. Eight out of ten participants had been involved in all of the four main themes.

Conclusion: The study demonstrated a substantial body of COVID-19 related work by SPHs in Europe, and an outstanding potential to deliver crucial knowledge and skills to support the governance and the public health systems necessary to combat COVID-19.
A Soft Systems Approach to Knowledge Worker Productivity: A Purposeful Activity Model for the Individual
Authors: Óskarsdóttir Helga Guðrún , Oddsson Guðmundur Valur , Sturluson Jón Þór , Sæmundsson Rögnvaldur Jóhann
Published: October 2021

This research attempted to find and define holistic systems that affect the productivity of the knowledge worker (KW), using the soft systems methodology (SSM). It is not enough to look at the management and improvement of knowledge worker productivity (KWP) from the viewpoint of the organization. The viewpoint of the individual KW needs to be considered as well. The KW owns the means of production; they carry their knowledge in their heads and take it with them when changing jobs. This paper proposes a conceptual framework that describes the process in which the KW uses resources to execute actions to create tangible or intangible artifacts with the intention of generating value. It was based on interpretations and inferences made from an extensive literature review using the snowballing method. This paper highlights what implications the lessons learned from the conceptual framework have on managing and improving KWP and delves deeper into four key concepts: value in knowledge work, knowledge, personal resources, and competencies.
Musculoskeletal Disorders in Agriculture: A Review from Web of Science Core Collection
Authors: Barneo-Alcántara Manuel , Díaz-Pérez Manuel , Gómez-Galán Marta , Carreño-Ortega Ángel , Callejón-Ferre Ángel-Jesús
Published: October 2021

Agricultural workers suffer from many work-related illnesses. Some of the most common include musculoskeletal disorders (MSDs). These types of disorders are caused by diverse factors. This research study intends to demonstrate the worldwide concern for MSD in agriculture. The objective is to compile and recognize in detail all the available studies on musculoskeletal disorders in agriculture developed in the world and by countries in the last 26 years. The Web of Science database was used for this purpose. Two hundred and fifty-six documents were found, of which 221 were finally selected for inclusion in the study. The studies span a period from 1995 to 2020. Of these, studies that were linked to improving sustainability stood out. Most of the research of this type emanates from developed countries. The conclusions to be drawn are that few countries pay attention to this area and that preventive measures are insufficient. Mechanization and improvement in work methods and organization are methods to reduce risks.
Antibiotic Consumption and Its Relationship with Bacterial Resistance Profiles in ESKAPE Pathogens in a Peruvian Hospital
Authors: Pérez-Lazo Giancarlo , Abarca-Salazar Susan , Lovón Renata , Rojas Rocío , Ballena-López José , Morales-Moreno Adriana , Flores-Paredes Wilfredo , Arenas-Ramírez Berenice , Illescas Luis Ricardo
Published: October 2021

A descriptive design was carried out studying the correlation between antimicrobial consumption and resistance profiles of ESKAPE pathogens (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter spp.) in a Peruvian hospital, including the surgical, clinical areas and the intensive care unit (ICU) during the time period between 2015 and 2018. There was a significant correlation between using ceftazidime and the increase of carbapenem-resistant Pseudomonas aeruginosa isolations (R = 0.97; p < 0.05) and the resistance to piperacillin/tazobactam in Enterobacter spp. and ciprofloxacin usage (R = 0.97; p < 0.05) in the medical wards. The Pseudomonas aeruginosa resistance to piperacillin/tazobactam and amikacin in the intensive care unit (ICU) had a significant reduction from 2015 to 2018 (67% vs. 28.6%, 65% vs. 34.9%, p < 0.001). These findings give valuable information about the rates and dynamics in the relationship between antibiotic usage and antimicrobial resistance patterns in a Peruvian hospital and reinforce the need for continuous support and assessment of antimicrobial stewardship strategies, including microbiological indicators and antimicrobial consumption patterns.
Combined Resistance to Ceftolozane-Tazobactam and Ceftazidime-Avibactam in Extensively Drug-Resistant (XDR) and Multidrug-Resistant (MDR) Pseudomonas aeruginosa: Resistance Predictors and Impact on Clinical Outcomes Besides Implications for Antimicrobial Stewardship Programs
Authors: Meschiari Marianna , Orlando Gabriella , Kaleci Shaniko , Bianco Vincenzo , Sarti Mario , Venturelli Claudia , Mussini Cristina
Published: October 2021

A retrospective case-control study was conducted at Modena University Hospital from December 2017 to January 2019 to identify risk factors and predictors of MDR/XDR Pseudomonas aeruginosa (PA) isolation with resistance to ceftazidime/avibactam (CZA) and ceftolozane/tazobactam (C/T), and of mortality among patients infected/colonized. Among 111 PA isolates from clinical/surveillance samples, 60 (54.1%) were susceptible to both drugs (S-CZA-C/T), while 27 (24.3%) were resistant to both (R-CZA-C/T). Compared to patients colonized/infected with S-CZA-C/T, those with R-C/T + CZA PA had a statistically significantly higher Charlson comorbidity score, greater rate of previous PA colonization, longer time before PA isolation, more frequent presence of CVC, higher exposure to C/T and cephalosporins, longer hospital stay, and higher overall and attributable mortality. In the multivariable analysis, age, prior PA colonization, longer time from admission to PA isolation, diagnosis of urinary tract infection, and exposure to carbapenems were associated with the isolation of a R-C/T + CZA PA strain, while PA-related BSI, a comorbidity score > 7, and ICU stay were significantly associated with attributable mortality. C/T and CZA are important therapeutic resources for hard-to-treat PA-related infections, thus specific antimicrobial stewardship interventions should be prompted in order to avoid the development of this combined resistance, which would jeopardize the chance to treat these infections.
Prevalence, Patterns, Association with Biofilm Formation, Effects on Milk Quality and Risk Factors for Antibiotic Resistance of Staphylococci from Bulk-Tank Milk of Goat Herds
Authors: Lianou Daphne T. , Petinaki Efthymia , Cripps Peter J. , Gougoulis Dimitris A. , Michael Charalambia K. , Tsilipounidaki Katerina , Skoulakis Anargyros , Katsafadou Angeliki I. , Vasileiou Natalia G. C. , Giannoulis Themis , Katsarou Eleni I. , Voidarou Chrysoula , Mavrogianni Vasia S. , Caroprese Mariangela , Fthenakis George C.
Published: October 2021

The objectives of this work were to study the prevalence and the patterns of antibiotic resistance of staphylococcal isolates from bulk-tank milk of goat herds across Greece, to assess possible associations of the presence of antibiotic resistance with the quality of milk in these herds and to evaluate herd-related factors potentially associated with the presence of antibiotic resistance among these staphylococcal isolates. A cross-sectional study was performed on 119 goat herds in Greece. Bulk-tank milk samples were collected for bacteriological examination; staphylococcal isolates were evaluated for resistance to 20 antibiotics. Oxacillin-resistant, resistant to at least one antibiotic, and multi-resistant staphylococcal isolates were recovered from 5.0%, 30.3%, and 16.0% of herds, respectively. Of 80 isolates, 7.5% were resistant to oxacillin, 50.0% were resistant to at least one antibiotic and 27.5% were multi-resistant. Resistance was seen more frequently among coagulase-negative staphylococci (59.3%) than among Staphylococcus aureus (23.8%). Resistance was more frequent against penicillin and ampicillin (41.3% of isolates) and fosfomycin (27.5%). No association was found with biofilm formation by staphylococci. For recovery of oxacillin-resistant isolates, the presence of working staff in the herds emerged as a significant factor; respective factors for the isolation of staphylococci resistant to at least one antibiotic were part-time farming and high (>10) number of systemic disinfections in the farm annually. The same three factors concurrently were also identified to be significant for the recovery of multi-resistant isolates.
Toxicity of Naphthenic Acids on the Chlorophyll Fluorescence Parameters and Antioxidant Enzyme Activity of Heterosigma akashiwo
Authors: Zhang Huanxin , Zhou Yumiao , Kong Qiang , Dong Wenlong , Lin Zhihao
Published: October 2021

Petroleum hydrocarbons can serve as a carbon source for marine phytoplankton; so, marine high-acid crude oil pollution events are likely to result in algal outbreaks or harmful algal blooms (HABs) in surface waters. Naphthenic acids (NAs) are the primary acidic component of crude oil, and red tide is of great concern due to its high diffusivity and strong destructive properties. It is important to study the mechanism of the toxic effect of NAs on the typical red tide algae, Heterosigma akashiwo, for the balance and stability of marine algae. The mechanism of NAs’ damage effect was investigated in terms of the antioxidant enzyme activity, cell number, the chlorophyll positive fluorescence parameters, and the cell morphology of microalgae. Experiments confirmed the hormesis of low-concentration (0.5, 2, and 4 mg/L) NAs on Heterosigma akashiwo, and the indicators of high-concentration (8 and 16 mg/L) NA exposures showed inhibition. In this study, the toxic effect of NAs on the target organism showed a clear concentration–dose relationship. The 16 mg/L NAs stress caused severe damage to the morphology and structure of the target biological cells in a short time (96 h), and the population growth decreased. The target organisms showed a staged oxidative stress response to NAs. The behavior in the low-concentration treatment groups showed toxicant excitatory effects on the photosynthetic efficiency and antioxidant enzyme activity of the target organisms. This study provides theoretical and practical data for the development of an important toxicological model of the toxicant’s excitement effects and antioxidant defense mechanisms. In addition, it provides prospective research data for the prediction and avoidance of ecological risk from NA pollution in marine environments.
ER-Mitochondria Calcium Flux by β-Sitosterol Promotes Cell Death in Ovarian Cancer
Authors: Bae Hyocheol , Park Sunwoo , Ham Jiyeon , Song Jisoo , Hong Taeyeon , Choi Jin-Hee , Song Gwonhwa , Lim Whasun
Published: October 2021

Phytosterols, which are derived from plants, have various beneficial physiological effects, including anti-hypercholesterolemic, anti-inflammatory, and antifungal activities. The anticancer activities of natural products have attracted great attention, being associated with a low risk of side effects and not inducing antineoplastic resistance. β-sitosterol, a phytosterol, has been reported to have anticancer effects against fibrosarcoma and colon, breast, lung, and prostate cancer. However, there are no reports of its activity against ovarian cancer. Therefore, we investigated whether β-sitosterol shows anticancer effects against ovarian cancer using human ovarian cancer cell lines. We confirmed that β-sitosterol induced the apoptosis of ovarian cancer cells and suppressed their proliferation. It triggered pro-apoptosis signals and the loss of mitochondrial membrane potential, enhanced the generation of reactive oxygen species and calcium influx through the endoplasmic reticulum-mitochondria axis, and altered signaling pathways in human ovarian cancer cells. In addition, we observed inhibition of cell aggregation, suppression of cell growth, and decreased cell migration in ovarian cancer cells treated with β-sitosterol. Further, our data obtained using ovarian cancer cells showed that, in combination with standard anti-cancer drugs, β-sitosterol demonstrated synergistic anti-cancer effects. Thus, our study suggests that β-sitosterol may exert anti-cancer effects against ovarian cancer in humans.
Choroidal Neovascularization Screening on OCT-Angiography Choriocapillaris Images by Convolutional Neural Networks
Authors: Taibouni Kawther , Miere Alexandra , Samake Abdourahmane , Souied Eric , Petit Eric , Chenoune Yasmina
Published: October 2021

Choroidal Neovascularization (CNV) is the advanced stage of Age-related Macular Degeneration (AMD), which is the leading cause of irreversible visual loss for elder people in developed countries. Optical Coherence Tomography Angiography (OCTA) is a recent non-invasive imaging technique widely used nowadays in diagnosis and follow-up of CNV. In this study, an automatic screening of CNV based on deep learning is performed using OCTA choriocapillaris images. CNV eyes (advanced wet AMD) are diagnosed among healthy eyes (no AMD) and eyes with drusen (intermediate AMD). An OCTA dataset of 1396 images is used to train and evaluate the model. A pre-trained convolutional neural network (CNN) is fine-tuned and validated on 80% of the dataset while the remaining 20% is used independently for predictions. The model can accurately detect CNV on the test set with an accuracy of 89.74%, precision of 0.96 and 0.99 area under the curve of the receiver operating characteristic. A good overall classification accuracy of 88.46% is obtained on a balanced test set. Detailed analysis of misclassified images shows that they are also considered ambiguous images for expert clinicians. This novel CNN-based application is truly a breakthrough to assist clinicians in the challenging task of screening for neovascular complications.
A Model for the Estimation of the Residual Driving Range of Battery Electric Vehicles Including Battery Ageing, Thermal Effects and Auxiliaries
Authors: Cannavacciuolo Gianmatteo , Maino Claudio , Misul Daniela Anna , Spessa Ezio
Published: October 2021

Sustainable mobility has recently become a priority of research for on-road vehicles. Shifting towards vehicle electrification is one of the most promising solutions concerning the reduction in pollutant emissions and greenhouse gases, especially for urban areas. Nevertheless, battery electric vehicles might carry substantial limitations compared with other technologies. Specifically, the electric range could be highly affected by the ageing process, non-optimal thermal management of the battery and cabin conditioning. In this paper, a model for the estimation of the residual range of electric vehicles is proposed accounting for the influence of battery state of health, battery pack temperature, power consumption of the main vehicle auxiliaries, and battery pre-heating on the residual driving range. The results of the model application to an L7 battery electric vehicle highlighted that the electric range can be highly affected by several factors related to real-world driving conditions and can consistently differ from nominal values.
Wear of 17-4 PH Stainless Steel Patterned Surfaces Fabricated Using Selective Laser Melting
Authors: Sanguedolce Michela , Zekonyte Jurgita , Alfano Marco
Published: October 2021

The recent developments in additive manufacturing (AM) are providing unprecedented opportunities in various fields, including the fabrication of advanced materials for tribological applications. The present work describes the results of an exploratory study focused on the analysis of 17-4 PH steel surfaces obtained using selective laser melting (SLM). In particular, the study includes the analysis of baseline (as-produced) and textured steel surfaces. Surface texturing comprises hexagonal prism structures (with or without dimples) arranged in a honeycomb pattern with 50 µm or 100 µm gap spacing. Starting from the minimum printing size enabled by the 3D printing platform, various textures are prepared by scaling up the characteristic dimensions of the prisms up to 500%. The obtained surface patterns are characterized (qualitatively and quantitatively) using a non-contact computerized numerical control (CNC) measuring system. The coefficient of friction (COF) was investigated using a Ball-on-Disk configuration using bearing steel balls as counterparts. For a fixed sliding speed, different contact loads and sliding radii were considered, while the tests were carried out in either dry or lubricant-impregnated conditions. The results of wear tests in both dry and lubricated conditions indicated that the baseline samples are provided with lower COF compared to the textured ones. For the latter, neither the gap spacing nor the presence of dimples led to significant variations in the COF. However, in lubricated conditions, the values of the COF for baseline and textured surfaces were closer and much smaller. In particular, the results provide clear indications regarding reducing the gap between prisms, which had a beneficial effect on the COF in lubricated conditions. Similarly, sensitivity to dimples was quite remarkable, with a reduction in the COF of about 30% when the larger gap spacing between the prisms was used.
A Hybrid MCDM Model for Live-Streamer Selection via the Fuzzy Delphi Method, AHP, and TOPSIS
Authors: Lim You Rang , Ariffin Aini Suzana , Ali Mazlan , Chang Kuei-Lun
Published: October 2021

The development of the Internet is a key revolution of the 20th century. The Internet has led to a boom in e-commerce. Online shopping is generally the most popular shopping option for consumers. The coronavirus disease 2019 (COVID-19) pandemic has resulted in adopting various measures, such as lockdown and stay-at-home orders, in various countries. This has led to changes in people’s consumption habits. In Taiwan, consumer behavior has also rapidly shifted to online shopping after the outbreak of the COVID-19. As livestreaming breaks time and space barriers, its real-time, interactive, and authentic features are unparalleled compared to other marketing methods, thereby creating a brand-new shopping experience for consumers. Accordingly, livestream shopping, a new consumption model, has developed and become an essential part of people’s daily lives. Live-streamers, who are similar to salespersons in traditional markets, play a vital role in e-commerce livestreaming. The success of livestream shopping has highlighted the importance of live-streamers. The competition among live-streamers has become more intense because of the arrival of many newcomers. Thus, operators must make careful hiring decisions. However, no related literature in the past has investigated this important topic. Therefore, this study applied a hybrid multi-criteria decision-making (MCDM) model comprising the fuzzy Delphi method (screen the selection criteria), analytic hierarchy process (AHP) (obtain the weight of each dimension and criterion), and technique for order preference by similarity to ideal solution (TOPSIS) (rank the alternatives) to assist the managers of shopping websites in selecting live-streamers. We interviewed the managers of shopping websites and reviewed the related literature to compile the selection criteria. Following the fuzzy Delphi method, 15 important selection criteria were retained based on the 30 managers’ opinions. Further, the criteria were classified into dimensions based on the previous literature and interviews conducted on managers to establish a hierarchical framework. On the basis of this hierarchical framework, AHP and TOPSIS were applied to help a case company select live-streamers. A comparative analysis between the outcomes from AHP and AHP/TOPSIS was also conducted in this study. This study is the first empirical study on live-streamers’ selection and adds to the literature on livestreaming.
The Effects of a Zirconia Addition on the Compressive Strength of Reticulated Porous Zirconia-Toughened Alumina
Authors: Lee Chae-Young , Lee Sujin , Ha Jang-Hoon , Lee Jongman , Song In-Hyuck , Moon Kyoung-Seok
Published: October 2021

Porous ceramics have attracted researchers due to their high chemical and thermal stability. Among various types of porous ceramics, reticulated porous ceramics have both high porosity and good permeability. These properties of porous ceramics are difficult to replace with porous metals and polymers. ZTA is used in a variety of applications, and a wealth of experimental data has already been collected. However, research reports on reticulated porous zirconia-toughened alumina (ZTA) are insufficient. Therefore, we prepared reticulated porous ZTA via the replica template method. In this study, various processing conditions (average particle size, zirconia content, solid loading, dispersant, and thickener) were adjusted to improve the compressive strength of the reticulated porous ZTA. As a result, the optimized processing conditions for improving the compressive strength of reticulated porous ZTA could be determined.
Effect of Magnetic Field on the Corrosion of API-5L-X65 Steel Using Electrochemical Methods in a Flow Loop
Authors: Parapurath Shahid , Ravikumar Arjun , Vahdati Nader , Shiryayev Oleg
Published: October 2021

Limited studies have been conducted on the effect of a magnetic field on the corrosion behavior of steels. Investigating the effect on pipeline material in the oil and gas industries will be beneficial regarding corrosion prediction and control. In this work, the effect of a magnetic field on the corrosion process of API 5L X65 carbon steel was investigated in a well-developed flow loop using potentiodynamic polarization curves and electrochemical impedance spectroscopy (EIS). Using permanent magnets and a well-designed corrosion electrode set-up, the corrosion mechanism of API 5L X65 steel was studied at different magnetic orientations and different flow conditions in a NaCl solution. The surface morphology of the corroded samples was studied using a scanning electron microscope, and the micro-morphologies of the corrosion deposits and the surface elemental composition were analyzed. The results show that the presence of a magnetic field increases the corrosion rate of API 5L X65 carbon steel, and that flow velocities and magnetic orientation have a significant influence on the anodic corrosion current. The results of the polarization curves indicate a negative shift in the Tafel curve, leading to an increase in the corrosion rate with the introduction of a magnetic field in the flow system. The results of the EIS show that the charge transfer rate is decreased when a magnetic field is applied. This work provides important direction in terms of the understanding of the combined effect of magnetism and flow on the corrosion in pipelines used in the oil and gas industries.
Assessing the Microbiological Safety Parameters of Minas Artisanal Cheese Samples in Retail Environments in São Paulo, Brazil
Authors: Allaion Josisleine Recalde , Barrionuevo Karina Ghougassian , Franco Bernadette Dora Gombossy de Melo
Published: October 2021

Minas artisanal cheese is the best known and most consumed type of cheese in Brazil. Prepared with raw bovine milk and starter cultures, these cheeses face many opportunities for post-processing contamination during their transport to commercial establishments as well as in how they are stored, displayed, and maintained for cutting/slicing at retail establishments. It is a common practice for retailers to purchase entire cheeses from the producers, cut them into smaller pieces for retail sale, and store them at room temperature instead of properly refrigerating them. This study evaluated the microbiological safety parameters of samples of Minas artisanal cheeses collected at retail establishments in the city of São Paulo, Brazil, to more realistically assess consumer exposure to the most common pathogens. Samples were submitted for investigation of Salmonella spp., Listeria monocytogenes, and the counts of total coliforms, Escherichia coli, and coagulase-positive enterotoxigenic staphylococci using culture and real-time PCR methods. A worrisome number of samples failed to comply with the current Brazilian legislation for foods in retail environments and presented more than one non-compliance issue. Results highlighted that quality and safety management tools, such as good hygiene practices and HACCP, in retail environments deserve more attention to reduce the possible risks to consumer health.
Current Status of Optical Systems for Measuring Lycopene Content in Fruits: Review
Authors: Villaseñor-Aguilar Marcos-Jesús , Padilla-Medina José-Alfredo , Botello-Álvarez José-Enrique , Bravo-Sánchez Micael-Gerardo , Prado-Olivares Juan , Espinosa-Calderon Alejandro , Barranco-Gutiérrez Alejandro-Israel
Published: October 2021

Optical systems are used for analysing the internal composition and the external properties in food. The measurement of the lycopene content in fruits and vegetables is important because of its benefits to human health. Lycopene prevents cardiovascular diseases, cataracts, cancer, osteoporosis, male infertility, and peritonitis. Among the optical systems focused on the estimation and identification of lycopene molecule are high-performance liquid chromatography (HPLC), the colorimeter, infrared near NIR spectroscopy, UV-VIS spectroscopy, Raman spectroscopy, and the systems of multispectral imaging (MSI) and hyperspectral imaging (HSI). The main objective of this paper is to present a review of the current state of optical systems used to measure lycopene in fruits. It also reports important factors to be considered in order to improve the design and implementation of those optical systems. Finally, it was observed that measurements with HPLC and spectrophotometry present the best results but use toxic solvents and require specialized personnel for their use. Moreover, another widely used technique is colorimetry, which correlates the lycopene content using color descriptors, typically those of CIELAB. Likewise, it was identified that spectroscopic techniques and multispectral images are gaining importance because they are fast and non-invasive.
Synthesis of Chitosan-Coated Silver Nanoparticle Bioconjugates and Their Antimicrobial Activity against Multidrug-Resistant Bacteria
Authors: Shinde Surbhi , Folliero Veronica , Chianese Annalisa , Zannella Carla , De Filippis Anna , Rosati Luigi , Prisco Marina , Falanga Annarita , Mali Avinash , Galdiero Marilena , Galdiero Massimiliano , Franci Gianluigi
Published: October 2021

The increase in multidrug-resistant bacteria represents a true challenge in the pharmaceutical and biomedical fields. For this reason, research on the development of new potential antibacterial strategies is essential. Here, we describe the development of a green system for the synthesis of silver nanoparticles (AgNPs) bioconjugated with chitosan. We optimized a Prunus cerasus leaf extract as a source of silver and its conversion to chitosan–silver bioconjugates (CH-AgNPs). The AgNPs and CH-AgNPs were characterized using transmission electron microscopy (TEM), dynamic light scattering (DLS), Fourier transform infrared spectroscopy (FT-IR), ultraviolet–visible spectroscopy (UV–Vis), and zeta potential measurement (Z-potential). The cytotoxic activity of AgNPs and CH-AgNPs was assessed on Vero cells using the 3-[4.5-dimethylthiazol-2-yl]-2.5-diphenyltetrazolium bromide (MTT) cell proliferation assay. The antibacterial activity of AgNPs and CH-AgNPs synthesized using the green system was determined using the broth microdilution method. We evaluated the antimicrobial activity against standard ATCC and clinically isolated multisensitive (MS) and multidrug-resistant bacteria (MDR) Escherichia coli (E. coli), Enterococcus faecalis (E. faecalis), Klebsiella pneumonia (K. pneumoniae), and Staphylococcus aureus (S. aureus), using minimum inhibitory concentration (MIC) assays and the broth dilution method. The results of the antibacterial studies demonstrate that the silver chitosan bioconjugates were able to inhibit the growth of MDR strains more effectively than silver nanoparticles alone, with reduced cellular toxicity. These nanoparticles were stable in solution and had wide-spectrum antibacterial activity. The synthesis of silver and silver chitosan bioconjugates from Prunus cerasus leaf extracts may therefore serve as a simple, ecofriendly, noncytotoxic, economical, reliable, and safe method to produce antimicrobial compounds with low cytotoxicity.