bluuebunny/crossref_metadata_2025 huggingface dataset:
The Lego hypothesis of tissue morphogenesis: stereotypic organization of parallel orientational cell adhesions for epithelial self‐assembly
Authors: Zhang Lili , Wei Xiangyun
Published: September 2024

How tissues develop distinct structures remains poorly understood. We propose herein the Lego hypothesis of tissue morphogenesis, which states that during tissue morphogenesis, the topographical properties of cell surface adhesion molecules can be dynamically altered and polarised by regulating the spatiotemporal expression and localization of orientational cell adhesion (OCA) molecules cell‐autonomously and non‐cell‐autonomously, thus modulating cells into unique Lego pieces for self‐assembling into distinct cytoarchitectures. This concept can be exemplified by epithelial morphogenesis, in which cells are coalesced into a sheet by many types of adhesions. Among them, parallel OCAs (pOCAs) at the lateral cell membranes are essential for configuring cells in parallel. Major pOCAs include Na+/K+‐ATPase‐mediated adhesions, Crumbs‐mediated adhesions, tight junctions, adherens junctions, and desmosomes. These pOCAs align in stereotypical orders along the apical‐to‐basal axis, and their absolute positioning is also regulated. Such spatial organization of pOCAs underlies proper epithelial morphogenesis. Thus, a key open question about tissue morphogenesis is how to regulate OCAs to make compatible adhesive cellular Lego pieces for tissue construction.
NMC‐4 Ameliorates Depression‐Like Behavior and Neuroinflammation Caused by Chronic Unpredictable Mild Stress
Authors: Chen Yawen , Gu Hongshun , Ye Danni , Li Yi , Chen Yan , Qiao Haoyi , Huang Yujiao , Tao Ran , Yu Shuhui , Zhang Jianjun , Fei Wenting , Wang Linyuan
Published: September 2024

Depression is a prevalent mental disorder, but the side effects of antidepressants also make depressed patients resistant. Effective and safe antidepressants should be developed from traditional herbs, with the aim of reducing the side effects of antidepressants and improving the efficacy of drugs. In this study, the new macamide compound‐4 (NMC‐4) was synthesized for the first time, addressing the problem of difficult extraction, isolation, and low content of natural macamide. NMC‐4 was characterized using mass spectrometry, nuclear magnetic resonance, and infrared spectroscopy. The protective effect of NMC‐4 against cell injury was demonstrated to be stronger than that of natural macamide (N‐benzylhexadecanamide, XA) using a PC12 cell injury model. The study explored the effects of NMC‐4 on chronic unpredictable mild stress (CUMS)‐induced depressive symptoms. NMC‐4 significantly improved depressive‐like behaviors. NMC‐4 ameliorated CUMS‐induced depressive‐like behaviors by mitigating neuroinflammation and modulating the NF‐κB/Nrf2 and BDNF/PI3K/Akt pathways.
Impact of the swallowable Allurion intragastric balloon on body weight and composition: An Ecuadorian experience
Authors: Patiño Araujo Bernarda , Duque Jácome Karla Elizabeth , Salgado Báez María Emilia , Villavicencio Logroño Gabriel , Salgado Macías Napoleón
Published: September 2024

People with excess weight and obesity compose 64.5% of the Ecuadorian population. The Allurion Intragastric Balloon (IGB) is a noninvasive weight loss alternative for patients ineligible for other bariatric procedures. The impact of the procedure on weight loss and body composition, along with insertion complications and secondary effects were investigated. This is a single‐centre retrospective study of patients who underwent Allurion IGB insertion from July 2020 to March 2021. This study followed 167 patients for 12 months after EIGB insertion. Our sample had a mean initial weight of 83.6 ± 13.8 kg and initial body mass index of 31.3 ± 3.6 kg/m2. Percentage total weight loss was 4.65% ± 0.56%, 8.5% ± 4%, 12.29% ± 4.65%, 15.68% ± 5.22%, 17.14% ± 6.05%, and 14.68% ± 18.02% for months 1, 2, 3, 4, 6 and 12, respectively. In the fourth month, 92 patients (56.4%) had lost ≥50% excess body weight; at month 6, 104 patients (67.5%) achieved this goal. Regarding body composition, body fat percentage started at 38.99% ± 7.92%, and at month 6 was 35.67% ± 6.84%, this was the only significance in the statistical analysis.This study aligns with the literature's safety and efficacy results for the Allurion IGB while illustrating the need for further research regarding weight behaviour after its expulsion.
Diagnostic utility of direct immunofluorescence test panels for cutaneous vasculitis: A scoping review
Authors: Lehman Julia S. , Ferringer Tammie C. , Fung Maxwell A. , Cassarino David S. , Shalin Sara C.
Published: September 2024

Background

Due to the immune‐mediated nature of non‐infectious cutaneous vasculitis, skin biopsy specimens are often submitted for direct immunofluorescence (DIF) testing when vasculitis is considered clinically. However, evidence regarding the clinical value of DIF has not been rigorously appraised.

Objective

In this scoping review, we aimed to systematically evaluate the peer‐reviewed literature on the utility of DIF in vasculitis to assist with the development of appropriate use criteria by the American Society of Dermatopathology.

Methods

Two electronic databases were searched for articles on DIF and vasculitis (January 1975–October 2023). Relevant case series involving more than or equal to three patients, published in English, and with full‐text availability were included. Additional articles were identified manually via reference review. Due to study heterogeneity, findings were analyzed descriptively.

Results

Of 255 articles identified, 61 met the inclusion criteria. Cumulatively representing over 1000 DIF specimens, several studies estimated DIF sensitivity to be 75%. While vascular immunoglobulin A (IgA) deposits on DIF were associated with renal disease, other systemic associations were inconsistent. Vascular IgG deposition may be overrepresented in ANCA‐associated vasculitis. Granular vascular and epidermal basement membrane zone Ig deposition differentiated hypocomplementemic from normocomplementemic urticarial vasculitis. Few studies have assessed the added value of DIF over routine microscopy alone in vasculitis.

Conclusions

This scoping review discovered that DIF testing for vasculitis has been performed not only for diagnostic confirmation of vasculitis but also for disease subtype classification and prediction of systemic associations. Future studies on test sensitivity of DIF compared to that of histopathology are needed.
Cofrogliptin once every 2 weeks as add‐on therapy to metformin versus daily linagliptin in patients with type 2 diabetes in China: A randomized, double‐blind, non‐inferiority trial
Authors: Ren Qian , Li Ling , Su Xiuhai , Hu Xiaolin , Qin Guijun , Han Jie , Liu Yu , Wang Junmin , Ji Linong
Published: September 2024

Aim

We evaluated the efficacy and safety of cofrogliptin, a novel dipeptidyl peptidase‐4 inhibitor taken once every 2 weeks (Q2W), compared with linagliptin (taken daily) in patients with type 2 diabetes inadequately controlled on metformin in China.

Materials and Methods

In this phase 3 randomized, double‐blind, active‐controlled, multicentre study, patients were randomly assigned 1:1:1 to receive cofrogliptin 10 mg Q2W, cofrogliptin 25 mg Q2W, or linagliptin 5 mg daily, all as an add‐on treatment to metformin, for 24 weeks. Eligible patients could enter an open‐label extension period and receive cofrogliptin 25 mg Q2W for an additional 28 weeks. The primary endpoint was change in glycated haemoglobin from baseline to 24 weeks, with a non‐inferiority margin of 0.4% for cofrogliptin versus linagliptin treatment.

Results

Overall, 465 patients entered the 24‐week treatment period (median age: 57.0 years). The least‐squares mean (standard error) change in glycated haemoglobin from baseline to week 24 was −0.96 (0.063), −0.99 (0.064) and −1.07 (0.065) for the cofrogliptin 10 mg, cofrogliptin 25 mg and linagliptin 5 mg groups, respectively. The between‐group difference met the predefined margin for non‐inferiority of cofrogliptin (10 and 25 mg) versus linagliptin treatment. The incidence of common adverse events (≥5% patients) during the 24‐week treatment period was similar between treatment groups. There were no serious hypoglycaemic events.

Conclusion

In Chinese patients with type 2 diabetes inadequately controlled on metformin, the glucose‐lowering effect of cofrogliptin (Q2W) was non‐inferior to linagliptin (daily), with a similar safety profile maintained over 52 weeks of treatment.
Neurovascular dysfunction in psychiatric disorders: Underlying mechanisms and therapeutic approaches
Authors: Sanches Eliane Swely , Simões Daniela , Baptista Filipa Isabel , Silva Ana Paula
Published: September 2024

Background

Neurovascular interfaces, specifically the blood–brain barrier (BBB) and blood–retinal barrier (BRB), play pivotal roles in maintaining the homeostasis of the central nervous system (CNS). For a long time, these structures were seen only as a way of protection, but we currently know that they have a critical role in CNS (dys)function. Several studies have identified neurovascular alterations in early stages of brain and eye diseases, contributing to the pathophysiology of such conditions. More recently, interesting data have also highlighted the importance of neurovasculature in psychiatric disorders.

Methods

Using the PubMed database, we brought together the evidence concerning the changes in BBB and BRB under psychiatric conditions, with a focus on anxiety, major depressive disorder (MDD), attention‐deficit/hyperactivity disorder (ADHD) and drug abuse, specifically related with methamphetamine (METH) and cocaine consumption.

Results

We summarized the main findings obtained from in vitro and animal studies, as well as clinical research that has been undertaken to identify neurovascular abnormalities upon such neuropsychiatric disorders. The drivers of barrier alterations were examined, namely the role of neuroinflammation, while reporting putative barrier‐associated biomarkers of these disorders.

Conclusion

This review underscores the critical need for a deeper understanding of BBB and BRB function in neuropsychiatric conditions and their potential as therapeutic targets while elucidating the key players involved. The innovative approaches to managing these complex disorders are also addressed while bridging the gap concerning what is currently known regarding the association between neuropsychiatric conditions and their vascular implications.
Spatial Abilities and Endodontic Access Cavity Preparation: Implications for Dental Education
Authors: Collet Philippine , Tra Robenson , Reitmann Anna , Valette Sébastien , Hoyek Nady , Maurin Jean‐Christophe , Ducret Maxime , Villat Cyril , Santamaria Julie , Richert Raphaël
Published: September 2024

Introduction

Access cavity preparation is a crucial step in root canal treatment but is one of the most complex procedures in the curriculum to learn, with students often reporting spatial orientation difficulties during drilling. The present study aimed to evaluate the influence of spatial abilities on the preparation of endodontic access cavities among third‐year dental students.

Materials and Methods

Students from Lyon dental faculty participated voluntarily. The mental rotation test (MRT) evaluated spatial ability. Students prepared access cavities on 3D‐printed mandibular molars, subsequently scanned and assessed against eight evaluation points, including morphology, canal access, floor preservation and convergence angle. Principal component analysis (PCA) assessed dataset variations.

Results

A total of 43 volunteers participated. PCA revealed two principal components accounting for 80.8% of variations: the first PC primarily consisted of MRT score (64.3%) and morphology (14.1%); the second comprised operative time (46.1%) and morphology (18.0%). There were significant differences in morphology based on MRT scores, but no correlation was found between other parameters.

Discussion

Lower MRT scores were associated with larger cavity preparations, raising questions about potential curriculum adaptations to enhance spatial reasoning. The operative time was not correlated with higher MRT scores but did contribute to variations in cavity morphology.

Conclusion

Spatial abilities have a substantial impact on the quality of endodontic access cavity preparations; further studies should evaluate if the incorporation of 3D atlas exercises could be beneficial.
Fully protected marine areas linked to reduced home ranges of fishes
Authors: Ohayon Sarah , Abecasis David , Almeida Pedro R. , Alós Josep , Aspillaga Eneko , Belo Ana Filipa , Costa José Lino , Di Franco Antonio , Di Lorenzo Manfredi , Ferguson Adrian , Guidetti Paolo , Kraft Sebastian , La Mesa Gabriele , Olsen Esben Moland , Parsons Darren , Pickholtz Renanel , Quintella Bernardo R. , Silva Ana Filipa , Taylor Brett M. , Villegas‐Ríos David , Belmaker Jonathan
Published: September 2024

Home range size is a fundamental trait that can affect the probability of fish being harvested and, at the same time, may be affected by fishing. The relationship between home range size and fishing will impact the effectiveness of fully protected areas (FPAs), as it will influence the number of fish moving into fished areas, affecting both spillover and edge effects. One hypothesis is that individuals within FPAs will present reduced home range size relative to individuals in fished areas. This pattern can be driven by demographic selection (e.g. fishing of individuals with large home ranges leaving the FPAs), improved habitat requiring less foraging movements, or behavioural changes associated with reduced fishing threats. To test the relationship between home range size and protection, we compiled 1143 individual‐level home range sizes based on acoustic tracking, covering 17 species from 11 FPAs in 7 countries, with information on distance from FPA borders. A dichotomic analysis (in/out of FPAs) did not support a significant change in the home range size between FPAs and fished areas. However, continuous analysis across the FPA borders demonstrated reduced home range size within the FPAs. We did not find an effect of FPA age or size on this pattern. While we cannot pinpoint the underlying mechanism for the pattern revealed, we suggest behavioural changes as the main driver for reduced home range within FPAs. This mechanism will lead to more resident populations within FPAs, reducing fishing mortality within FPAs yet limiting spillover benefits to adjacent fisheries.
Synthesising a network of evidence on a seabird bycatch mitigation measure
Authors: Gilman Eric , Chaloupka Milani , Debski Igor , Kim Mi Ae , Kingma Eric , Ochi Daisuke
Published: September 2024

Robust estimates of the relative efficacies of alternative management interventions are essential for developing evidence‐informed fisheries bycatch policy. Bycatch is a major threat to the conservation of albatrosses and other pelagic seabirds. Branchline weighting is one approach prescribed by regional fisheries management organisations and the Agreement on the Conservation of Albatrosses and Petrels to reduce seabird bycatch in pelagic longline fisheries. We used a Bayesian multilevel network meta‐regression modelling approach to conduct the first synthesis of available evidence to assess the relative efficacies at mitigating seabird bycatch of alternative pelagic longline weighting designs. Unlike conventional pairwise meta‐analysis, network meta‐analysis enables the simultaneous comparison of multiple interventions within a coherent modelling framework. There was a > 97% probability that all weighting designs significantly reduced seabird bycatch compared to a reference design with no weight within 5 m of the hook. Nonetheless, some weighting designs were significantly more effective at reducing seabird bycatch than others—for instance, the 2 designs with weights >60 g and >1 m from the hook performed the best with >93% probability that those 2 designs performed significantly better than 2 more commonly used designs with less weight but attached closer to the hook. These two best performing designs reduced seabird bycatch by ca 89% relative to the reference design. These relative efficacies and rankings, when combined with other performance criteria such as costs to commercial viability and crew safety, support robust evaluations of alternative bycatch management strategies.
Tuning residual stresses in welded structures by exploiting bio‐inspired suture interfaces
Authors: Ferro Paolo , Tang Keke , Berto Filippo , Salvati Enrico
Published: September 2024

Nature offers a valuable source of inspiration when designing optimized structures. For instance, when two or more shells are joined during growth processes, Nature prefers to exploit interlocking paradigms to enhance the strength of the bond—cranial sutures are one example. Mimicking this distinctive characteristic when welding thin metallic elements may offer some structural benefits. The present study theoretically investigates the possibility of mitigating the detrimental tensile residual stress that always originated by welding processes, by exploiting Computational Welding Mechanics. Specifically, two plates joined by laser welding following sinusoidal paths (i.e., wave‐like), alongside a linear one, will be studied and compared. Thanks to the distinctive temporal and spatial heat distributions of sinusoidal welding, both welding stress and strain develop in dissimilar ways compared with linear path welding—this is reflected to the resulting weldment distortion too. The results show that geometrically optimized sinusoidal welding can effectively reduce residual stress magnitudes compared with the linear configuration counterpart. The implications of these findings are of particular interest when dealing with the structural performance of welded structures, especially in fatigue contexts.
A Close‐to‐Optimal Discretization Strategy for Pumping Test Numerical Simulation
Authors: Figueroa Ronny , Bresciani Etienne
Published: September 2024

Numerical modeling offers a valuable alternative to analytical solutions for pumping test analysis. However, little is known about how discretization impacts results accuracy and runtime. This study presents a systematic method for defining the spatiotemporal discretization of pumping test numerical models based on dimensionless parameters. Two types of analysis are considered: one where observations are made in the pumping well, and another one where observations are made in different wells. The influence of the discretization parameters on results accuracy and runtime is investigated and an optimal set of parameters is determined that minimizes runtime while maintaining the maximum error under 1% for an “average” aquifer. Lower runtimes are achieved when the analysis focuses on the pumping well, which is attributed to the steady‐state analytical solution approximating drawdown in the well in the numerical scheme employed. Additional tests demonstrate the robustness of the derived set of parameters in different configurations.
Effects of pre‐deacidification on 3‐MCPDE and GE contents of high‐acid oils during refining
Authors: Kantekin‐Erdogan Meryem Nur , Emektar Kubra , Yorulmaz Asli , Tekin Aziz
Published: September 2024

Crude fats and oils may have high free fatty acid (FFA) content depending on raw material quality and processing conditions. Their refining is always a challenge due to refining loss and the formation of some process contaminants such as 3‐monochloropropane‐1,2‐diol esters (3‐MCPDE), and glycidyl esters (GE). In this study, crude hazelnut oil having 12.10% FFA was degummed, bleached, and pre‐deacidified by a molecular distillation (MD) unit before neutralisation and deodorisation. Changes in FFA, diglyceride(DG), 3‐MCPDE, GE, and tocopherol contents were monitored. Almost no changes were detected in FFA and DG contents during degumming and bleaching while the latter resulted in the 3‐MCPDE formation and GE reduction. Some tocopherol losses were also observed after these steps. On the other hand, a huge amount of FFA was distilled (from 12.10% to 1.71%) by the MD process performed at 200 °C, 0.25 mbar, as well as with a 53.6% loss of tocopherol. The samples were then subjected to deodorisation before (MD‐PHY) and after neutralisation (MD‐CHE) at 230 °C under 2 mbar absolute pressure for 2, 4, and 8 h. While some 3‐MCPDE and GE formations were detected during MD‐CHE, there were almost no formations in these contaminants after MD‐PHY. When the bleached oil having 12.10% FFA was also directly deodorised at given conditions, no formations but some reductions in 3‐MCPDE and GE contents of the oils were determined, possibly because of esterification of FFA with DGs that are an important precursor of these contaminants.
Opinion on the prospects of emerging food processing technologies to achieve sustainability in the industry by reduced energy consumption, waste reduction and valorisation, and improved food nutrition
Authors: Gavahian Mohsen
Published: September 2024

Conventional food processing technologies cannot meet the demands of sustainability in the face of societal concerns about climate change, resource scarcity, and food insecurity. Therefore, the food industry must embrace emerging food processing technologies such as ohmic heating, microwave heating, cold plasma, pulsed electric field, and ultrasound to achieve sustainable food production. Although these technologies have been researched worldwide, they have yet to be widely adopted in the industry as concerns such as high capital investments, regulatory issues, technological limitations, and scalability limit their practical applications. This manuscript discusses key contributions of emerging technologies in enhancing sustainability, including reducing energy consumption, waste reduction and valorisation, and enhancing nutrition. Despite these promising capabilities, fully benefiting from emerging technologies in the industry requires further technological advancements, optimisation, economic viability, consumer acceptance, regulatory compliance, and sustainable workforce education. These could be achieved only by close collaborations between academia, industry, and technology developers. Such considerations and actions to resolve the concerns could facilitate the industrial application of emerging technologies to achieve a more sustainable future with reduced carbon emission, environmental impact, and carbon footprint, along with the capability of improved resource efficiency and further contributions to food security and food nutrition improvement.
Effects of pH, kappa‐carrageenan concentration and storage time on the textural and rheological properties of pea protein emulsion‐gels
Authors: Cappa Evangelina R. , Sponton Osvaldo E. , Olivares Maria Laura , Santiago Liliana G. , Perez Adrián A.
Published: September 2024

This study examined the rheological and textural properties of gels produced through emulsion gelation using pea protein isolate (PPI). Emulsions were prepared with varying PPI concentrations (2%–10%) and 0.25 oil fraction, followed by heat treatment. Higher PPI concentrations improved encapsulation efficiency (EE), but heat slightly reduced it, though EE remained above 90%. Droplet size decreased with higher PPI. The emulsion was then gelled with different kappa‐carrageenan (KC) concentrations (1%–2%) and pH levels (4–7). Gel hardness increased with KC content, while adhesiveness decreased. Springiness, cohesiveness and water‐holding capacity (WHC) rose with pH and KC, while meltability decreased. Rheological tests showed stronger gels with higher KC and lower pH. Storage time generally increased gel strength, except at pH 4. This work highlights the interplay between PPI, KC, pH and storage time in shaping the properties of pea protein‐based emulsions and gels.
Production of starch edible films using chitin nanocrystals extracted from mushroom
Authors: Mirpourian Nastaran Sadat , Fathi Milad , Maleky Farnaz
Published: September 2024

This study aimed to produce chitin nanocrystals (CHNC) from mushroom and investigate their potential application in improving physicochemical properties of starch edible films. Alpha‐Chitin was extracted by demineralisation, deproteination, and discoloration treatments of mushroom powder, while CHNC were obtained by acid hydrolysis. Hydrolysis using hydrochloric acid for 120 min at concentration of 3 m was selected as the best conditions for CHNC. X‐ray Diffraction Analysis and Transmission Electron Microscopy showed a crystallinity index of 77 ± 1.2% and needle‐like morphology for CHNC with an average length of 81 nm and diameter of 19 nm. Chemical, morphological, and thermal properties of CHNC were characterised using FTIR, SEM, and DSC analysis and results indicated an increase of the purity of CHNC after hydrolysis. Different concentrations of CHNC (1, 3, 5, and 7% W/W) were used to produce a nanocomposite green film from corn starch. Quantification of the mechanical properties of the green films documented a direct relationship between CHNC concentration and the samples tensile strength and Young's modulus. However, increasing CHNC concentration resulted in a reduction in the samples' water vapour permeability, water solubility, and moisture absorption. The results of this study are promising, suggesting enhancements in the physicochemical properties of the edible film through CHNC incorporation.
Examination of diet quality and alcohol on serum IgG levels after first and second COVID‐19 vaccines
Authors: Cesur Fatih
Published: September 2024

Rationale

The change of IgG of COVID‐19 vaccine was thought to be an effect of diet quality or daily habits.

Aims and Objectives

This study aimed to correlate diet quality and healthy living factors with serum IgG response in the blood.

Methods

Participants were selected from volunteers who had their first vaccination and did not have COVID‐19 disease (Male = 21 Female = 40). Serum IgG levels were measured on average (avg) 28 days after the COVID‐19 vaccine. Information was obtained directly from the participants by questionnaire method (Food consumption record, frequency of food consumption, Diet Quality Index [(DQI], etc.).

Results

A significant difference was observed in the IgG levels of the second measurement of age (young/middle) and gender (male/female) (p < 0.05). A significant difference was found in the first measurement of serum IgG levels and IgG avgs of those with medium diet quality and those who did not drink alcohol (p < 0.05). When the IgG2/1 ratio was examined between alcohol users and nonalcohol, a significant increase was observed about two times in non‐alcohol users (p = 0.039). There is a positive significant moderate strength relationship between the second measurements of IgG and anthropometric measurements and the first, second, and avg measurements of IgG with DQI. It was found that there was a negative significant medium‐strength relationship between individuals' amount of alcohol consumption and IgG avg (r = −0.535, p = 0.009).

Conclusions

Medium diet quality has been seen to affect antibody levels positively. At the same time, it is thought that alcohol use negatively affects serum IgG antibody response in the long term. Other than that, there was shown to be a correlation between IgG levels and DQI.
Distinct characteristics of various autoimmune liver diseases: A 22‐year hospital‐based study in Taiwan
Authors: Chang Ming‐Ling , Le Puo‐Hsien , Chen Wei‐Ting , Chen Tai‐Di , Su Chung‐Wei , Chen Cheng‐Jen , Lin Cheng‐Yu , Wu Chi‐Huan , Kuo Chia‐Jung , Sung Kei‐Feng , Chien Rong‐Nan
Published: September 2024

Background and Aim

The characteristics of autoimmune liver diseases (AILDs), including primary biliary cholangitis (PBC), autoimmune hepatitis (AIH), and PBC‐AIH overlap syndrome (OS), have rarely been investigated and compared in Asia.

Methods

At the Taiwan tertiary referral center, 330 PBC patients (87% treated with ursodeoxycholic acid [UDCA]), 143 AIH patients (94.4% treated with immunosuppressive therapy [IST]) and 21 PBC‐AIH OS patients (85.7% treated with UDCA and IST) were enrolled.

Results

Compared with AIH patients, PBC patients were older at baseline and had greater female‐to‐male sex ratios, alkaline phosphatase (ALP) and γ‐glutamyl transferase (γ‐GT) levels, and liver cirrhosis (LC), dyslipidemia, and hepatic and cardiometabolic complication rates. PBC patients had the lowest transaminase levels, whereas AIH patients had the highest transaminase levels. PBC patients had greater 22‐year all‐cause mortality and liver transplantation (ACMaLT) (43.5 vs 25.4%, P = 0.004), LC (75 vs 58.5%, P < 0.01), dyslipidemia (54.4 vs 45.9%, P = 0.001), and cerebrovascular accident (11.3 vs 0.8%, P = 0.019) cumulative incidences (CIs) than did AIH patients; PBC‐AIH OS patients had greater systemic lupus erythematosus (28.9 vs 8.9%, P = 0.009) CI than did PBC patients. Baseline ALP (hazard ratio: 1.001), albumin (0.514), platelet count (0.997), and LC (3.438) were associated with ACMaLT; age (1.110), albumin (0.350), cirrhosis (46.219), and hepatitis C virus antibody positivity (5.068) were associated with hepatocellular carcinoma (HCC); and female sex (2.183) and body mass index (1.054) were associated with autoimmune diseases.

Conclusions

Compared with AIH patients, PBC patients had greater cardiometabolic CI, and ACMaLT CI, which was associated with cholestasis, liver functional reserve and LC. Older AILD patients with LC and females with obesity demand special caution for the development of HCC and extrahepatic autoimmune diseases, respectively.
Unveiling the applicability of the Brussels Infant and Toddler Stool Scale for hard stool in young children: A South Asian perspective
Authors: Bui Hung Manh , Trinh Oanh Thi Hoang , Lin Shyh‐Hsiang , Nguyen‐Hoang Anh , Le Nguyen Quoc Khanh , Nguyen Nha Tran , Nguyen Ngan Thi Kim
Published: September 2024

Background and Aim

The Rome IV criteria, the standard for diagnosing functional constipation (FC), deem the Bristol Stool Scale (BSS) unsuitable for assessing stool consistency in young children. Hence, the Brussels Infant and Toddler Stool Scale (BITSS) was developed. We aimed to validate and test the reliability of BITSS for hard stools and FC among infants and toddlers, where there is limited evidence in Asian populations.

Methods

The research evaluated FC in children aged 0–48 months who came for medical examination using Rome IV criteria. Stool properties provided by caregivers were assessed sequentially through three methods: the BSS, the BITSS, and caregiver reports.

Results

A total of 370 responses were received, with an average age of 26.2 months. Substantial agreement was observed between the BITSS and caregiver reports for hard stools (concordance rate: 91.9%, κ = 0.75), while near‐perfect agreement was found between BITSS and BSS (concordance rate: 93.5%, κ = 0.81). The BITSS exhibited higher sensitivity than the BSS in assessing hard stools (95.3% vs 87.5%, P < 0.001). And the BITSS (23.5%) identified the highest prevalence of FC than the BSS (20.5%) and caregiver report (18.7%), with near‐perfect agreement. Moderate agreement was reported when evaluating the test–retest reliability between BITSS and caregiver reports (concordance rate: 86.2%, κ = 0.44).

Conclusions

The BITSS, more sensitive than the BSS in identifying abnormal, especially hard stools, aids in early FC detection in young children. These findings support using BITSS over BSS for evaluating hard stools in infants and toddlers, both in Vietnam and globally.
Spatiotemporal trends in the burden of colorectal cancer incidence and risk factors at country level from 1990 to 2019
Authors: Li Quanmei , Zhang Xiaorui , Wang Yijie , Gao Ran , Zhang Yijun , Zheng Xite , Huang Fengyi , Liu Wanqi , Luo Chuning , Liu Fen
Published: September 2024

Background and Aim

Worldwide, the incidence of colorectal cancer (CRC) continues to rise and remains a major public health concern. This study aimed to analyze the temporal and spatial trends in CRC incidence and related risk factors at the country level.

Methods

Data on CRC and related risk factors were obtained from the Global Burden of Disease Study (GBD) 2019 study. Temporal trends were evaluated using estimated annual percentage change while spatial trends were analyzed using spatial autocorrelation and autoregression. Additionally, linear mixed‐effects models were employed to identify risk factors linked to CRC incidence.

Results

Globally, from 1990 to 2019, the incidence cases of CRC increased by 157.23%. At the national level, the incidence of CRC increased in most countries, with the highest increases of age‐standardized incidence rate (ASIR) in Equatorial Guinea, Vietnam, and China. In both 1990 and 2019, global spatial clustering of CRC ASIR highlighted hotspots in Europe, characterized by elevated CRC ASIR levels. A comparative analysis of risk factors between hotspot countries and others indicated that gender and alcohol use exerted greater influence in hotspots than elsewhere.

Conclusion

Although from 1990 to 2019, the highest growth in ASIR of CRC has been observed in African, Asian, and Latin American countries, the hotspots are still concentrated in Europe. In the identified hotspots, gender and alcohol use exert a more significant impact on CRC incidence compared with other countries. Thus, we should pay attention to countries where the CRC incidence is increasing and these risk factors.
End‐of‐life emergency department use and healthcare expenditures among older adults: A nationally representative study
Authors: Gettel Cameron J. , Kitchen Courtney , Rothenberg Craig , Song Yuxiao , Hastings Susan N. , Kennedy Maura , Ouchi Kei , Haimovich Adrian D. , Hwang Ula , Venkatesh Arjun K.
Published: September 2024

Background

Emergency department (ED) visits at end‐of‐life may cause financial strain and serve as a marker of inadequate access to community services and health care. We sought to examine end‐of‐life ED use, total healthcare spending, and out‐of‐pocket spending in a nationally representative sample.

Methods

Using Medicare Current Beneficiary Survey data, we conducted a pooled cross‐sectional analysis of Medicare beneficiaries aged 65+ years with a date of death between July 1, 2015 and December 31, 2021. Our primary outcomes were ED visits, total healthcare spending, and out‐of‐pocket spending in the 7, 30, 90, and 180 days preceding death. We estimated a series of zero‐inflated negative binomial models identifying patient characteristics associated with the primary outcomes.

Results

Among 3812 older adult decedents, 610 (16%), 1207 (31.7%), 1582 (41.5%), and 1787 (46.9%) Medicare beneficiaries had ED visits in the final 7, 30, 90, and 180 days, respectively, of life. For Medicare beneficiaries with at least one ED visit in the final 30 days of life, the median total and out‐of‐pocket costs were, respectively, $12,500 and $308, compared, respectively, with $278 and $94 for those without any ED visits (p < 0.001 for both comparisons). Having a diagnosis of dementia (odds ratio [OR] 0.71; 95% confidence interval [CI] 0.51–0.99; p = 0.04) and being on hospice status during the year of death (OR 0.56; 95% CI 0.48–0.66; p = <0.001) were associated with a decreased likelihood of having an ED visit. Having dementia was associated with a decreased likelihood of having any healthcare spending (OR 0.50; 95% CI 0.36–0.71; p = 0.001) and any out‐of‐pocket spending (OR 0.51; 95% CI 0.36–0.72; p = <0.001).

Conclusions

One in three older adults visit the ED in the last month of life, and approximately one in two utilize ED services in the last half‐year of life, with evidence of associated considerable total and out‐of‐pocket spending.
Dyadic examination of parents' general psychological distress and coparenting in families with young children: The mediating role of couple satisfaction
Authors: Güre Pelin , Gürmen M. Selenga , Acar İbrahim H.
Published: September 2024

The current research explored the dyadic relationships between general psychological distress (GPD) and coparenting dimensions (cooperation, conflict, triangulation) through the mediation of couple satisfaction among parents with young children. The sample comprised 184 heterosexual couples (184 mothers, 184 fathers, age range from 25 to 57 years) married for 10 years on average. The actor–partner interdependence model (APIM) and APIM Mediation Model analyses demonstrated significant relationships between mothers' and fathers' GPD and all three of their own coparenting dimensions (direct actor effects), also through their own couple satisfaction (indirect actor–actor effects). Additionally, mothers' GPD had direct effects on fathers' coparenting cooperation (partner effect). Fathers' GPD had significant indirect effects on all dimensions of mothers' coparenting through mothers' couple satisfaction (partner–actor effects), plus on mothers' coparenting triangulation through fathers' couple satisfaction (actor–partner effect). Findings were in line with Family System Theory and consistent with prior research. Clinical implications were discussed.
Experiences, health needs, and access to health services of university students with female circumcision: A qualitative study
Authors: Evrenol Öçal Simge , Şimşek Çetinkaya Şahika
Published: September 2024

Aims

This study aimed to assess the female circumcision (FC) experiences, healthcare needs, and access to health services of foreign university students who are victims of female genital mutilation and studying in Turkey.

Methods

This descriptive qualitative study was conducted in the Faculty of Health Science in a state university in Turkey from December 2021 to January 2022. Eight students who had been subjected to FC participated in this study. Data were collected by conducting in‐depth, semistructured online interviews and using a pilot‐tested interview guide. The data were analyzed using inductive thematic analysis.

Results

Four themes emerged: “reasons for performing FC, ‘health effects of FC,’ ‘psychological problems linked to FC,’ and ‘FC survivors' access to health services.’” Students stated that they exposed to this practice because of limiting their womanhood, they experienced problems, such as pelvic pain, infection, loss of sexuality, fear of pregnancy and birth, and they had healthcare needs due to FC but face some barriers.

Conclusions

FC negatively affects women's health and has specific healthcare needs. Studying in a different country can be turned into an opportunity to solve the health problems they experience. For this, the right approach with knowledgeable and culturally sensitive health professionals can change stereotypes towards practice.
Voicing expertise: Exploring strategic use of vowel variants in the English pronunciation of Chinese language instructors
Authors: Mei Yunbo
Published: September 2024

Accent often carries social implications and can serve as an identity marker, reflecting how speakers perceive themselves and how they wish to be perceived by others. This study employs a variationist approach to examine the agency of Chinese English language teachers in negotiating their professional identities through accent. Instead of a loose association between identity and accent, detailed sociophonetic analyses reveal that these teachers construct desired self‐representations through the strategic use of linguistic resources. Findings indicated that participants’ perceptions of the relationship between teacher qualifications and native‐like/first‐language‐influenced English accent can predict their pronunciation patterns. Relating to how they perceive nativeness and professional identity, participants’ use of robust DRESS–TRAP nuclei and larger tongue movements in MOUTH and PRICE can be interpreted as strategies to distance themselves from a “non‐native” identity, which is often stigmatized within the language teaching community. The utilization of stylistic resources allows participants to construct a professional teacher persona and signify expertise in language teaching.
Characterisation of Fusarium and Neocosmospora Species Associated With Crown Rot and Wilt of African Eggplant (Solanum aethiopicum) in Ghana
Authors: Okorley Benjamin Azu , Ravnskov Sabine , Brentu Francis C. , Offei Samuel K.
Published: September 2024

African eggplant (AEP) (Solanum aethiopicum group Gilo) is an important vegetable with considerable economic value in Ghana and tropical Africa. However, fungal diseases threaten its cultivation. Surveys conducted in 2021 and 2022 growing seasons across 35 commercial farms in five regions of Ghana revealed symptoms of crown rot and wilt affecting AEP. This study was undertaken to identify and characterise 36 fungal isolates causing these diseases in AEPs using morphological, molecular and pathogenicity assays. Morphological and molecular analyses of the Btub2, Tef‐1α and Rpb2 sequences identified two Fusarium species (F. elaeidis and F. fredkrugeri) and three Neocosmospora species (N. falciforme, N. suttoniana and N. solani) associated with the plant diseases. F. elaeidis (14 isolates) and N. falciforme (14) were the most commonly isolated species from symptomatic plants. Specifically, F. elaeidis was found in wilting plants, while F. fredkrugeri and the three Neocosmospora spp. were more associated with wilting plants with crown rot symptoms than plants with only wilt symptoms. All identified species exhibited pathogenicity when inoculated onto AEP roots and stems, confirming field observations. F. elaeidis was the most aggressive in inducing wilt symptoms, while N. solani and N. suttoniana were particularly aggressive in inducing crown rot symptoms. This study is the first to document that F. elaeidis, F. fredkrugeri, N. falciforme and N. suttoniana are pathogens causing wilt and crown rot in AEP in Ghana. These findings provide essential insights for developing effective disease management strategies to reduce losses from these fungal species.
Clinical and pathological characteristics of a dog with scleromyxoedema
Authors: Guaguère E. , Husson J. C. , Degorce‐Rubiales F. , Delverdier M. , Muller A.
Published: September 2024

An 8‐year‐old male cross‐Labrador retriever was presented for a progressive appearance of folds all over the body of the dog. Scleromyxoedema was diagnosed based on clinical signs and histopathological features. Clinical signs were characterised by a papular and vesicular eruption and severe skin thickening causing exuberant folds along with concurrent severe osteoarthritis of the coxofemoral joints. Thyroid disorders were excluded and the condition was not associated with monoclonal gammopathy. Histopathological features consisted of mucin deposition, fibroblast proliferation and fibrosis. Prednisolone was prescribed to decrease mucin synthesis which allowed a marked clinical improvement. Due to the progressive inability to walk, the dog was euthanased 6 months after the first consultation.
Napping during cognitive behavioural therapy for insomnia: Friends or foes?
Authors: Faraut Brice , Gaillard Louise , Labonne Annabelle , Dubois Julie Margrethe , Adrien Joëlle , Léger Damien
Published: September 2024

Cognitive and Behavioural Therapy for Insomnia (CBT‐I) is the gold standard treatment for chronic insomnia, with one crucial step being the restriction of time spent in bed. This restriction often intensifies early afternoon sleepiness, leading to a natural gateway for a short recuperative nap, which might foster adherence to CBT‐I over time. In practice, mental health professionals providing CBT‐I lack consensus on whether or not to tolerate short naps during the CBT‐I period for requesting patients. In this pilot study, we examined the effects of authorised napping on CBT‐I efficiency in patients with insomnia (a napping group was compared with a matched non‐napping group, n = 108). We report that napping enhanced early afternoon alertness and importantly did not affect CBT‐I‐mediated improvements in the Insomnia Severity Index and Beck Depression Inventory‐2 and in self‐reported sleep efficiency, latency, and wake after sleep onset (assessed by the sleep diaries). Further investigations using objective methods of sleep assessments are now needed to confirm that napping behaviour does not compromise the improvements enabled by CBT‐I and may even strengthen adherence to the treatment.
Macroscopic and histopathological description of gastric lesions in horse, donkey, and mule fetuses in the last trimester of gestation
Authors: Medina‐Bolívar Angie Lorena , Faleiros Rafael Resende , Martínez‐Aranzales José Ramon
Published: September 2024

Background

Limited information is available on gastric diseases in neonatal foals as compared with extensive studies in young, adult, and geriatric horses. Reports on fetuses are scarce.

Objectives

Assess at necropsy stomachs of horse, donkey and mule fetuses in the third trimester of gestation to characterize lesions present during intrauterine life.

Animals

Forty‐six fetal stomachs from both sexes (21 horses, 21 donkeys, and 4 mules) in the third trimester of gestation were collected from a processing plant immediately after slaughter.

Methods

Measurements of longitudinal and transverse axes, weight and volume and gastric fluid pH were taken, and glandular and squamous mucosae were inspected. All findings of the gastric mucosa and measurements of the stomachs were presented descriptively. Groups were compared statistically, with significance level set at P < .05 for all evaluations.

Results

All gastric contents had pH >5.8, and mules had larger stomachs and higher weights compared with horses (P < .05). Macroscopic lesions were classified as hyperemic, punctate, and erosive. Histopathologically, lesions were consistent with a chronic inflammatory process.

Conclusions and Clinical Importance

Our study provides evidence of macroscopic and histopathological lesions in the gastric mucosae of equid fetuses in the last trimester of gestation. Relevant information for perinatology and neonatology is provided regarding the prevalence and classification of preulcerous lesions in equids before birth.
New insights into the reproductive physiology and culture approaches of Paratilapia sp., a Malagasy cichlid with potential interest for sustainable aquaculture
Authors: Caballero‐Huertas Marta , Canonne Marc , Rakotomanana Domoina , Tianandriatsiferana Rovaniaina Jospin Césaire , Andrianjafisoa Tsiriniaina , Rasoloariniaina Jean Robertin , Mortillaro Jean‐Michel
Published: September 2024

One of the primary objectives in freshwater aquaculture is the search for sustainable systems based on agroecological intensification. In this context, the cultivation of local species is decisive, although requiring the identification of new candidates. Malagasy aquaculture is an example of the development of such an approach. We aimed to deepen the knowledge of the biology of the Paratilapia sp., specifically, their reproductive traits and zootechnical aspects, with special emphasis on the juvenile stages. The information provided has interest for its commercial production and the species conservation, as populations are subjected to overexploitation and habitat degradation. This study is a consolidated report on morphological characteristics, sexual dimorphism, reproductive biology, and early developmental ontogeny. The main results suggested a marked sexual dimorphism, as well as greater growth of males compared with females. Aggressive behavior between males was observed before pair formation. Generally, female and males provided protection until fry are independent. Females produced 2756 ± 598 eggs per spawn on average (gonadosomatic index = 4.6%), with a reproductive frequency of one to two spawns. Despite the need to improve breeding protocols, this work represents a breakthrough in producing a local freshwater species of potential interest.