bluuebunny/crossref_metadata_2025 huggingface dataset:
Role of Promising Secondary Metabolites to Confer Resistance Against Environmental Stresses in Crop Plants: Current Scenario and Future Perspectives
Authors: Chen Delai , Mubeen Bismillah , Hasnain Ammarah , Rizwan Muhammad , Adrees Muhammad , Naqvi Syed Atif Hasan , Iqbal Shehzad , Kamran Muhammad , El-Sabrout Ahmed M. , Elansary Hosam O. , Mahmoud Eman A. , Alaklabi Abdullah , Sathish Manda , Din Ghulam Muhae Ud
Published: May 2022

Plants often face incompatible growing environments like drought, salinity, cold, frost, and elevated temperatures that affect plant growth and development leading to low yield and, in worse circumstances, plant death. The arsenal of versatile compounds for plant consumption and structure is called metabolites, which allows them to develop strategies to stop enemies, fight pathogens, replace their competitors and go beyond environmental restraints. These elements are formed under particular abiotic stresses like flooding, heat, drought, cold, etc., and biotic stress such as a pathogenic attack, thus associated with survival strategy of plants. Stress responses of plants are vigorous and include multifaceted crosstalk between different levels of regulation, including regulation of metabolism and expression of genes for morphological and physiological adaptation. To date, many of these compounds and their biosynthetic pathways have been found in the plant kingdom. Metabolites like amino acids, phenolics, hormones, polyamines, compatible solutes, antioxidants, pathogen related proteins (PR proteins), etc. are crucial for growth, stress tolerance, and plant defense. This review focuses on promising metabolites involved in stress tolerance under severe conditions and events signaling the mediation of stress-induced metabolic changes are presented.
LcNAC13 Is Involved in the Reactive Oxygen Species-Dependent Senescence of the Rudimentary Leaves in Litchi chinensis
Authors: Wang Congcong , Liu Hao , Huang Lijie , Chen Houbin , Lu Xingyu , Zhou Biyan
Published: May 2022

Litchi is an important evergreen fruit tree. Floral formation in litchi is induced by low temperatures (LTs). However, unstable flowering is a challenge for litchi production in times of global warming and climate change. Previous studies have shown that the methyl viologen dichloride hydrate-generated reactive oxygen species (ROS) could promote flowering. Leaves in the panicles may affect the development of the inflorescence in litchi under high-temperature condition. In this study, potted litchi trees were transferred to growth chambers at LT and high temperature (HT). From a previous dataset of the RNA sequencing of the ROS-treated rudimentary leaves, a NAC transcription factor-encoding gene LcNAC13 was identified. By genetic transformation of LcNAC13 to Arabidopsis thaliana and tobacco, it was found that the ROS-induced senescence of the leaves was accelerated. Silencing LcNAC13 by virus-induced gene silencing (VIGS) delayed ROS-dependent senescence. Our results suggested that LcNAC13 regulates rudimentary leaf senescence. Our study provided a new target gene for the future molecular breeding of new cultivars that could flower under global warming conditions.
Molecular Determinants of in vitro Plant Regeneration: Prospects for Enhanced Manipulation of Lettuce (Lactuca sativa L.)
Authors: Bull Tawni , Michelmore Richard
Published: May 2022

In vitroplant regeneration involves dedifferentiation and molecular reprogramming of cells in order to regenerate whole organs. Plant regeneration can occur via two pathways,de novoorganogenesis and somatic embryogenesis. Both pathways involve intricate molecular mechanisms and crosstalk between auxin and cytokinin signaling. Molecular determinants of both pathways have been studied in detail in model species, but little is known about the molecular mechanisms controllingde novoshoot organogenesis in lettuce. This review provides a synopsis of our current knowledge on molecular determinants ofde novoorganogenesis and somatic embryogenesis with an emphasis on the former as well as provides insights into applying this information for enhancedin vitroregeneration in non-model species such as lettuce (Lactuca sativaL.).
Flavonoid Synthesis and Metabolism During the Fruit Development in Hickory (Carya cathayensis)
Authors: Chen Jia-Hui , Hou Na , Xv Xv , Zhang Da , Fan Tong-Qiang , Zhang Qi-Xiang , Huang You-Jun
Published: May 2022

Hickory (Carya cathayensis) kernel is rich in powerful bioactive flavonoids, which can remove excess free radicals in the human body and play an important role in regulating the physiological metabolism of the plant. This study investigated the changes of flavonoids in hickory exocarp and embryo during development. In this study, 72 DEGs involved in the regulation of flavonoid biosynthesis in fruits were identified, and TT4, CCoAOMT1, UGT71D1, C4H, F3H, TT8, FLS1, and LDOX were identified as the core genes of flavonoid biosynthesis. A total of 144 flavonoid-related metabolites were detected by metabolite analysis. Transcriptome and metabolome analysis combined to construct the flavonoid biosynthesis regulatory pathway in the development stage of hickory fruit. Our results provide a theoretical basis for the exploration and regulation of functional genes related to flavonoid biosynthesis and metabolism in hickory and other plants and the breeding of new walnut varieties.
Democracy and Technocracy in Sweden's Experience of the COVID-19 Pandemic
Authors: Andersson Staffan , Aylott Nicholas , Eriksson Johan
Published: May 2022

Sweden's management of the coronavirus pandemic, beginning in early 2020, has been much discussed because it deviated from other countries' equivalents. Set in the context of scholarly debate about the balance between politicians and experts in political decision-making, we argue that a necessary condition for this case of Swedish exceptionalism was the manner of policy-making adopted by the Swedish authorities. In this article, we describe this policy-making procedure, which involved a radical form of delegation by elected politicians to appointed experts, and seek to explain how it came about. We focus on the 1st year of the pandemic, and use media reports and other public documents, including parts of a public inquiry, as our empirical material.
Previous Experience Seems Crucial to Eliminate the Sex Gap in Geometry Learning When Solving a Navigation Task in Rats (Rattus norvegicus)
Authors: Aguilar-Latorre Alejandra , Romera-Nicolás Víctor , Gimeno Elisabet , Chamizo V. D.
Published: May 2022

There is much evidence, both in humans and rodents, that while navigating males tend to use geometric information whereas females rely more on landmarks. The present work attempts to alter the geometry bias in female rats. In Experiment 1 three groups of female rats were trained in a triangular-shaped pool to find a hidden platform, whose location was defined in terms of two sources of information, a landmark outside the pool and a particular corner of the pool. On a subsequent test trial with the triangular pool and no landmark, females with prior experience with two other pool shapes–with a kite-shaped pool and with a rectangular-shaped pool (Group Long Previous Experience, LPE), were significantly more accurate than control rats without such prior experience (Group No Previous Experience, NPE). Rats with a short previous experience–with the rectangular-shaped pool only (Group Short Previous Experience, SPE) did not differ from Group NPE. These results suggest that the previous experience with different shaped-pools could counteract the geometry bias in female rats. Then, Experiment 2A directly compared the performance of LPE males and females of Experiment 1, although conducting several test trials (i.e., shape, landmark, and preference). The differences between males and females disappeared in the three tests. Moreover, in a final test trial both males and females could identify the correct corner in an incomplete pool by its local, instead of global, properties. Finally, Experiment 2B compared the performance of NPE rats, males and females, of Experiment 1. On the test trial with the triangular pool and no landmark, males were significantly more accurate than females. The results are explained in the framework of selective attention.
Administrative Ethics Conflict and Governance of Grassroots Government Staff Under the Human Relationship Society
Authors: Yin Yue , Li Taotao , Yang Fan
Published: May 2022

The conflict of administrative morality among civil servants at the grassroots level arises from the background of China’s long-standing traditional culture, and the current administrative system cannot keep up with the pace of economic development. In the process of grassroots management, due to the lag in the construction of administrative morality, the traditional official standard thinking, the imperfection of the current system, and the restriction of human nature, it is easy to cause the administrative moral conflict of the grassroots civil servants in practice. This paper takes the interpersonal society as the research background, analyzes the influence of the interpersonal society on the environment, and studies the administrative ethics conflicts and governance issues of basic civil servants from the interpersonal background. In addition, this paper conducts a more detailed field investigation based on fuzzy cluster analysis, analyzes the manifestations and causes of the administrative moral conflict of grassroots civil servants in the context of human society from multiple perspectives and levels, and then proposes countermeasures to solve the administrative moral conflict. The administrative moral conflict and governance of grass-roots civil servants under the background of interpersonal relationship is of great positive significance for solving the administrative moral conflict and improving the service awareness and service level of grass-roots civil servants.
Effects of Including Gender Pronoun Questions in Surveys
Authors: Palanica Adam , Lopez Luke , Gomez Amy , Fossat Yan
Published: May 2022

This research examines whether the mere presence of asking about gender pronouns (e.g., she/her, he/him, they/them, and ze/zir) in a survey enhances participants’ attitudes and satisfaction of answering the questions. A large sample (N = 1,511) of heterosexual, cisgender, and LGBTQIA+ participants across the United States (US) were surveyed an online “personality test” (as a deception), with the real purpose of examining whether asking a pronoun question enhanced their perceptions of the survey. Three demographic groups were included: (i) heterosexual–cisgender (n = 503), (ii) gay–cisgender (n = 509), and (iii) genderqueer (trans, non-conforming, other, n = 499). Half of each group were randomly given either a survey that included a gender pronoun question (test) or not (control), and then all rated their perceptions of the survey questions. For participants who identified as heterosexual or gay, no major differences were found between survey conditions. However, participants who identified as genderqueer experienced significant increases of satisfaction, comfort level, and perceived relevance of the questions when given a survey that asked their gender pronouns versus the survey that did not. These findings have implications for any surveys that ask about personal demographics, and suggest that any form of written communication should include clarity about gender pronouns.
High Emotional Similarity Will Enhance the Face Memory and Face-Context Associative Memory
Authors: An Shu , Zhao Mengyang , Qin Feng , Zhang Hongchi , Mao Weibin
Published: May 2022

Previous research has explored how emotional valence (positive or negative) affected face-context associative memory, while little is known about how arousing stimuli that share the same valence but differ in emotionality are bound together and retained in memory. In this study, we manipulated the emotional similarity between the target face and the face associated with the context emotion (i.e., congruent, high similarity, and low similarity), and examined the effect of emotional similarity of negative emotion (i.e., disgust, anger, and fear) on face-context associative memory. Our results showed that the greater the emotional similarity between the faces, the better the face memory and face-context associative memory were. These findings suggest that the processing of facial expression and its associated context may benefit from taking into account the emotional similarity between the faces.
Untying Moral Efficacy and Meaningfulness in Promoting Students’ Social Entrepreneurial Intentions: The Mediating Role of Positive Reciprocity
Authors: Xiang Jian , Zhang Yanjun
Published: May 2022

This study chooses to describe social entrepreneurship as a social mission that enables business students to identify opportunities to launch start-ups and social enterprises by understanding the nature of social responsibility and fostering a reciprocal attitude to solve social issues. We collected data on students from different business schools in mainland China through a structured questionnaire (n = 326) and analyzed them through structural equation modeling (SEM). The results show that moral efficacy and meaningfulness are positively related to positive reciprocity, which leads to students’ social entrepreneurial intentions. The study concluded that potential social entrepreneurs should take ethical courses as part of their training to help them develop a responsible mentality and social entrepreneurial aspirations. On this basis, the practical and theoretical significance of this study is proposed, and its limitations and future development directions are pointed out.
Integration of Urine Proteomic and Metabolomic Profiling Reveals Novel Insights Into Neuroinflammation in Autism Spectrum Disorder
Authors: Liu Wenlong , Li Liming , Xia Xiaochun , Zhou Xulan , Du Yukai , Yin Zhaoqing , Wang Juan
Published: May 2022

Autism spectrum disorder (ASD) comprises a group of neurodevelopmental disorders whose etiology and pathogenesis are not fully understood. To gain insight into the molecular basis of ASD, we performed comparative integrated proteomic and metabolomic analyses of urine samples from children diagnosed with ASD and healthy children. All 160 samples underwent proteomics analysis and 60 were analyzed by liquid chromatography-mass spectrometry to obtain metabolite profiles. We identified 77 differentially expressed proteins (DEPs; 21 downregulated and 56 upregulated) and 277 differentially expressed metabolites; 31 of the DEPs including glutathione, leukocyte antigens, glycoproteins, neural adhesion factors, and immunoglobulins, have been implicated in neuroinflammation. The proteomic analysis also revealed 8 signaling pathways that were significantly dysregulated in ASD patients; 3 of these (transendothelial leukocyte migration, antigen processing and presentation, and graft vs. host disease) were associated with the neuroimmune response. The metabolism of tryptophan, which is also related to the neuroimmune response, has been found to play a potential role in ASD. Integrated proteome and metabolome analysis showed that 6 signaling pathways were significantly enriched in ASD patients, 3 of which were correlated with impaired neuroinflammation (glutathione metabolism, metabolism of xenobiotics by cytochrome P450 and transendothelial migration of leukocyte). We also found a correlation between prostaglandin (PG) E2 levels and the inflammatory response in ASD. These results underscore the prominent role of the neuroimmune response in ASD and provide potential biomarkers that can be used for diagnosis or as targets for early intervention.
The Development of a Short Chinese Version of the State-Trait Anxiety Inventory
Authors: Du Qingyun , Liu Haowen , Yang Chengjiao , Chen Xiaoyu , Zhang Xiaoyuan
Published: May 2022

Background

Few studies on anxiety in China have used the full version of the Spielberger State-Trait Anxiety Inventory (STAI) due to its length, despite its numerous advantages. The goal of this study was to develop a short Chinese version of the STAI and examine its reliability and validity among the general Chinese population and psychiatric patients diagnosed with anxiety.

Method

A total of 1,142 participants were invited to test the short Chinese version of the STAI; item analysis, validity testing and internal consistency reliability analysis were performed. Subsequently, 40 participants were enrolled to assess retest reliability 1 week later. Finally, 330 participants, including 33 psychiatric patients with anxiety, were used to assess the criterion and empirical validity. The Self-Rating Anxiety Scale (SAS) and Satisfaction with Life Scale (SWLS) were used as criteria, and receiver operating characteristic (ROC) analysis was conducted to evaluate the discrimination of the short version of the STAI between the groups with and without anxiety disorders.

Result

The short Chinese version of the STAI contains six items for each subscale (STAI-S-6 and STAI-T-6). The Pearson correlation coefficients between the two shortened Chinese versions of the STAI and the full-length STAI were 0.970 and 0.962, the Cronbach’s α coefficients were 0.871 and 0.842, and the retest reliability values were 0.728 and 0.813 (p < 0.001). Confirmatory factor analysis showed that the 2-factor model achieved an adequate model fit: for the STAI-S-6, CFI = 0.986, TLI = 0.974, and RMSEA = 0.075, and for the STAI-T-6, CFI = 0.994, TLI = 0.988, and RMSEA = 0.052. The short Chinese version of the STAI had a significant positive correlation with the SAS score (r = 0.289, 0.313; p < 0.001) and a negative correlation with the SWLS score (r = −0.476, 0.554; p < 0.001). A significant difference in the level of anxiety was found between participants with and without anxiety disorders. The diagnostic accuracy of the STAI-S-6 and STAI-T-6 for major anxiety disorder was acceptable, with areas under the ROC curves of 0.798 and 0.745, respectively.

Conclusion

The short Chinese version of the STAI demonstrates sound psychometric properties and is applicable in evaluating the level of anxiety in Chinese populations.
Cognitive and Experienced Flexibility in Patients With Anorexia Nervosa and Obsessive Compulsive Disorder
Authors: Sternheim Lot Catharina , van Passel Boris , Dingemans Alexandra , Cath Danielle , Danner Unna Nora
Published: May 2022

Objective

Anorexia nervosa (AN) and obsessive-compulsive disorder (OCD) share a neuropsychological profile characterized by cognitive inflexibility as evident in set-shifting problems, and by strong detail focus. Clinically, both patient groups display a strong rigidity which may be explained by these neurocognitive difficulties. Cognitive inflexibility may hinder treatment uptake and help explain suboptimal treatment outcomes in both AN and OCD. This is the first study to compare clinical AN and OCD groups andto examine similarities and differences in cognitive flexibility. Specifically, this study aims to investigate neuropsychological outcomes and self-reported difficulties in both clinical groups and a control group, and explore associations between the different flexibility outcomes and illness.

Method

Two hundred participants (61 AN, 72 OCD and 67 HC) performed neuropsychological tasks on set-shifting abilities (Trail Making Task, Stroop color-word interference, Intradimensional-Extradimensional shift task), detail focus (Group Embedded Figures Test) and self-reported set-shifting abilities and attention to detail (DFlex).

Results

Similarities between patient groups were found in terms of reduced set-shifting ability on the Trail Making Task and detail focus. Moreover, both patient groups self-reported more set-shifting problems but a less strong detail focus than HC, which in turn were not related to neuropsychological task outcomes in either of the groups. In both patient groups longer illness duration was associated to longer reaction times in the switching tasks and for both groups symptom severity was associated to higher experienced inflexibility and attention to detail.

Conclusion

Cognitive inflexibility processes are largely similar in patients with AN and OCD. Both patient groups report inflexibility, yet this is unrelated to neuropsychological outcomes. Illness duration seems to contribute to poorer set-shifting and higher illness severity is linked to more experienced inflexibility. Findings highlight the need for entangling different domains of cognitive flexibility and detail focus and examining self-report measures for a cohesive understanding of clinically relevant flexibility weaknesses in AN and OCD.
Switzerland’s Dependence on a Diamorphine Monopoly
Authors: Schmitt-Koopmann Caroline , Baud Carole-Anne , Junod Valérie , Simon Olivier
Published: May 2022

In 2021, the manufacturer of diamorphine reported a possible impending shortage for Switzerland and Germany. This led us to investigate this controlled medicine’s manufacture, market, and regulatory constraints. Based on our analysis of legal texts and gray literature in the form of reports and documents, we propose recommendations to prevent and address diamorphine shortages in Switzerland. Diamorphine, also known as pharmaceutical “heroin,” is used medically to treat persons with severe opioid use disorder in a handful of countries. The controlled medicine is manufactured from morphine, which, in turn, is extracted from opium poppies. Studying data from the International Narcotics Control Board for 2019, we find that Switzerland accounts for almost half of the worldwide medical consumption of diamorphine. It manufactures more than half of the worldwide total and keeps the largest stocks. Moreover, Switzerland is dependent on a sole supplier of diamorphine (monopoly). As a niche product, diamorphine has an increased risk of shortage. Such a shortage would immediately threaten a valuable public health program for around 1,660 Swiss patients. We believe it is urgent to curtail the monopoly and ensure a stable supply for the future.
Improving Communication of Public Health Bachelor's Degree Programs Through Visual Curriculum Mapping
Authors: Nelson-Hurwitz Denise C. , Tagorda Michelle , Patil Uday , Kehl Lisa
Published: May 2022

Undergraduate students balance course requirements for the university, college, school, and major. Each set of requirements, including degree-specific curriculum, is intended to promote synergistic interaction of competence, skills, and knowledge, beyond serving as a collection of individual courses. Understanding of curriculum is important for program recruitment as undergraduate students are more informed when deciding between bachelor's degrees options. Among cohorted programs, this understanding is also helpful in communicating and promoting common intellectual experiences. Comprehension of curriculum is especially important for persistence when students are better able to articulate the connections between course and competencies needed to advance in coursework. To improve universal design for learning within program advising, visual curriculum maps were created as infographics to support student understanding of Bachelor of Arts in Public Health degree requirements and specific capstone course pathways. This map is printed as a small booklet and has been pilot tested among prospective students with positive feedback, then implemented in routine advising sessions. Visual maps of capstone requirements were well-received in concept, however constructive student feedback during pilot testing necessitated further revision. Student feedback also encouraged the application of culturally appropriate visuals and analogies to celebrate student diversity. Visual aids such as these may improve access to information among students through universal design, and also improve recruitment, retention efforts, and student buy-in to degree curricula.
What Can Movements Teach Us About Brain Function?
Authors: Kinder Kaleb T. , DiMercurio Abigail , Buss Aaron T.
Published: May 2022

Humans typically make thousands of movements every day, which allow us to navigate and interact with the world around us. The brain controls the body’s movements and, amazingly, can instruct the body to move in just a split second. Even though the brain can rapidly move the body, most of our movements appear very smooth and efficient. While some movements are easy or automatic, such as blinking our eyes, other movements are more challenging, such as moving the arm to choose between two toys. Scientists know that the brain functions in separate ways for different types of movements. Hidden in our movements, however, lies valuable information that can provide clues about how the brain works. Scientists are using a recent technological advance, called movement tracking, to better understand brain functions such as decision making, attention, and memory.
Psychological Safety in High-Performance Sport: Contextually Applicable?
Authors: Taylor Jamie , Collins Dave , Ashford Michael
Published: May 2022

In recent years, high-performance sport has seen a rising interest in Psychological Safety, a construct with a strong empirical basis in certain business contexts. As research and practice interest grows in PS, there are early indications of practitioners and, to a lesser extent research, treating the construct as being universally transferable. We offer three central concerns with this situation. Firstly, it seems that a variety of different interpretations in use may limit the practical application of the construct. Secondly, a concern that not all dimensions of PS are transferable or applicable in the HPSs context, especially for athletes. Finally, emerging evidence from outside of sport suggests potential downsides to the perceptions of PS in a performance/selection sets. We suggest that, as with all theories and constructs, there is a pressing need for nuance and context-specific evidence in how researchers and practitioners approach transferability plus, perhaps, a little more understanding of the real-world high-performance context.
Transurethral Incision of the Bladder Neck at Three Points with a Needle-Type Electrode for Bladder Neck Contracture
Authors: Cao Guihua , Liu Liangcheng , Du Jianping , Li Wei , Li Qiang , Luo Na , Liu Xun , Zhou Junjie , Wu Tao
Published: May 2022

Purpose

This study aims to evaluate the efficacy of transurethral incision of the bladder neck (TUIBN) at three points with a needle-type electrode for treatment of bladder neck contracture (BNC).

Materials and Methods

Between January 2016 and April 2021, the bladder necks of 53 patients with BNC after surgery were incised by the needle-type electrode at the 5, 7, and 12 O’clock positions. Patient’s preoperational characteristics, peri- and postsurgical outcomes, such as time of operation, postoperative bladder irrigation, and postoperative hospital stay, and data of the international prostate symptom score (IPSS), maximum flow rate (Qmax), and postvoid residual (PVR) were recorded 3 and 6 months after surgery.

Results

All 53 cases of BNC were successfully treated in 35.00 (25.00, 45.00) min with 18.00 (14.00, 21.00) h for postoperative bladder irrigation with little intraoperative bleeding (less than 50 mL). The postoperative hospital stay ranged from 2 to 8 days, a mean of 3.50 (3.00, 5.00) days. No major intraoperative or postoperative complications were observed. All cases that underwent follow-up assessment at 3 and 6 months after the surgery showed significantly decreased IPSS and PVR and increased Qmax compared to preoperation ones (p ≤ 0.001). Of these 53 patients, there was no recurrence in severe BNC patients, but 5 of 53 (9.4%) BNC patients developed BNC again within 6 months and required repeated TUIBN. Thirty patients comprised five recurrent cases with a follow-up period of more than 1 year.

Conclusions

TUIBN at three points provides a safe, effective, and reliable option in treating patients with BNC.
Case Report: First Documented Hip Arthroplasty on Chinese Patient with Ochronotic Arthropathy
Authors: Ying Zhibo , Lu Jufeng , Wang Xucheng , Zeng Qinghe , Jin Hongting , He Bangjian
Published: May 2022

Alkaptonuria (AKU) is a rare autosomal recessive disorder caused by homogentisc acid (HGA) accumulation, the deposition of which in the joints usually causes ochronotic arthropathy. With no specific therapy for AKU currently, total joint arthroplasty in ochronotic arthropathy is applied to relieve the symptoms. A 63-year-old female patient came to our Orthopedic Surgery Department in 2019, complaining of severe limitation of movement and pain in the right hip for more than one year. A right total hip arthroplasy (THA) was performed due to the ineffective conservative therapy. At a follow-up of more than 15 months, the woman had full mobility with no complaining of pains. Since there is no relevant case reported about THA therapy for Chinese AKU patients, this report provides a feasible scheme, which makes clinical data more comprehensive.
Bibliometric Analysis of Mexican Publications on Stereotactic and Functional Neurosurgery From 1949 to 2021
Authors: Carrillo-Ruiz José Damián , Armas-Salazar Armando , Navarro-Olvera José Luis , Beltrán Jesús Q. , Bowles Brigham , González-Garibay Guillermo , Lee Ángel
Published: May 2022

Background

Stereotactic and functional neurosurgery (SFN) is a rapidly evolving field and some emerging countries, especially Mexico, have made significant contributions to this discipline. A bibliometric analysis has never been performed in Latin America, and this would be particularly important to show the areas that remain poorly studied, and design research strategies for the future.

Methods

Scopus was queried using keywords pertaining to functional neurosurgery, restricting the affiliation country to Mexico, and considering documents published after 1949. Added to the initial search, a complementary literature exploration by author, considering the publications of the most productive neurosurgeons, was performed. A descriptive statistical analysis was carried out.

Results

From 5,109 articles, only 371 were eligible. Scientific production has gradually increased with time. Epilepsy (31%) and movement disorders (27.4%) were the most studied neurological conditions, whereas the other 41.6% corresponded to pain, behavior disorders, spinal cord injuries, neuromodulation, stereotactic biopsies, and SFN history. Level of evidence was predominantly level V (59.1%). Publication output is highly skewed to Mexico City, which represents 78.4% of national production. Relative to factors associated with impact of research, publications in English had more citations (28.5 mean citations per paper), and journals with an impact factor greater than one had more than 10 mean citations per paper.

Conclusions

Mexico has experienced an increase in the productivity of SFN literature, addressing the most prevalent issues in the country (epilepsy and motor disorders). However, it is necessary to report studies with a higher level of evidence, as well as to decentralize the research collaborating with national institutions outside Mexico City. On the other hand, it is imperative to promote scientific production in English and in high-impact indexed journals to increase the visibility of our production. We would like to call upon our colleagues in other countries to reproduce our methodology, in order to determine the factors associated with the impact and productivity on SFN research.
The Exploration of miRNAs From Porcine Fallopian Tube Stem Cells on Porcine Oocytes
Authors: Fu Tzu-Yen , Wang Shu-Hsuan , Lin Tzu-Yi , Shen Perng-Chih , Chang Shen-Chang , Lin Yu-Han , Chou Chih-Jen , Yu Yu-Hsiang , Yang Kuo-Tai , Huang Chao-Wei , Shaw Steven W. , Peng Shao-Yu
Published: May 2022

Fallopian tube is essential to fertilization and embryonic development. Extracellular vesicles (EVs) from Fallopian tube containing biological regulatory factors, such as lipids, proteins and microRNAs (miRNAs) serve as the key role. At present, studies on oocytes from porcine oviduct and components from EVs remain limited. We aim to explore the effect of EVs secreted by porcine fallopian tube stem cells (PFTSCs) on oocyte. When the fifth-generation PFTSCs reached 80–90% of confluency, the pig in vitro maturation medium was utilized, and the conditioned medium collected for oocyte incubations. To realize the functions of EVs, several proteins were used to determine whether extracted EVs were cell-free. Field emission scanning electron microscope and nanoparticle tracking analyzer were used to observe the morphology. By next generation sequencing, 267 miRNAs were identified, and those with higher expression were selected to analyze the Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment maps. The selected miR-152-3p, miR-148a-3p, miR-320a-3p, let-7f-5p, and miR-22-3p, were predicted to target Cepb1 gene affecting MAPK pathway. Of the five miRNAs, miR-320a-3p showed significant difference in maturation rate in vitro maturation. The blastocyst rate of pig embryos was also significantly enhanced by adding 50 nM miR-320a-3p. In vitro culture with miR-320a-3p, the blastocyst rate was significantly higher, but the cleavage rate and cell numbers were not. The CM of PFTSCs effectively improves porcine oocyte development. The miRNAs in EVs are sequenced and identified. miR-320a-3p not only helps the maturation, but also increases the blastocyst rates.
Enhancing Algal Yield and Nutrient Removal from Anaerobic Digestion Piggery Effluent by an Integrated Process-Optimization Strategy of Fungal Decolorization and Microalgae Cultivation
Authors: Qian Jun , Zhang Jiaqi , Jin Zeyu , Cheng Jiali , Li Jingjing , Song Hanwu , Lu Qian , Li Hugang , Wan Ting , Fu Siyi , Li Jun , Zhou Wenguang
Published: May 2022

The dark brown anaerobic digestion piggery effluent (ADPE) with a large amount of ammonium generally needs high dilution before microalgae cultivation due to its inhibiting effects on algal growth. Due to the strong decolorization of fungi by degrading organic compounds in wastewater, the process-optimization integrated strategy of fungal decolorization of ADPE and subsequent microalgae cultivation with ammonium-tolerant strain may be a more reliable procedure to reduce the dilution ratio and enhance algal biomass production, and nutrient removal from ADPE. This study determined a suitable fungal strain for ADPE decolorization, which was isolated and screened from a local biogas plant, and identified using 26s rRNA gene sequence analysis. Subsequently, ADPE was pretreated by fungal decolorization to make low-diluted ADPE suitable for the algal growth, and conditions of microalgae cultivation were optimized to achieve maximum algal yield and nutrient removal from the pretreated ADPE. The results showed one promising locally isolated fungal strain, Nanchang University-27, which was selected out of three candidates and identified as Lichtheimia ornata, presenting a high decolorization to ADPE through fungal pretreatment. Five-fold low-diluted ADPE pretreated by L. ornata was the most suitable medium for the algal growth at an initial concentration of ammonium nitrogen of 380 mg L−1 in all dilution treatments. Initial optical density of 0.3 and pH of 9.0 were optimal culture conditions for the algal strain to provide the maximum algal yield (optical density = 2.1) and nutrient removal (88%, 58%, 65%, and 77% for the removal rates of ammonium nitrogen, total nitrogen, total phosphorus, and chemical oxygen demand, respectively) from the pretreated ADPE. This study demonstrated that fungal decolorization and subsequent microalgae cultivation could be a promising approach to algal biomass production and nutrient removal from ADPE.
Neutrophils and Asthma
Authors: Yamasaki Akira , Okazaki Ryota , Harada Tomoya
Published: May 2022

Although eosinophilic inflammation is characteristic of asthma pathogenesis, neutrophilic inflammation is also marked, and eosinophils and neutrophils can coexist in some cases. Based on the proportion of sputum cell differentiation, asthma is classified into eosinophilic asthma, neutrophilic asthma, neutrophilic and eosinophilic asthma, and paucigranulocytic asthma. Classification by bronchoalveolar lavage is also performed. Eosinophilic asthma accounts for most severe asthma cases, but neutrophilic asthma or a mixture of the two types can also present a severe phenotype. Biomarkers for the diagnosis of neutrophilic asthma include sputum neutrophils, blood neutrophils, chitinase-3-like protein, and hydrogen sulfide in sputum and serum. Thymic stromal lymphoprotein (TSLP)/T-helper 17 pathways, bacterial colonization/microbiome, neutrophil extracellular traps, and activation of nucleotide-binding oligomerization domain-like receptor family, pyrin domain-containing 3 pathways are involved in the pathophysiology of neutrophilic asthma and coexistence of obesity, gastroesophageal reflux disease, and habitual cigarette smoking have been associated with its pathogenesis. Thus, targeting neutrophilic asthma is important. Smoking cessation, neutrophil-targeting treatments, and biologics have been tested as treatments for severe asthma, but most clinical studies have not focused on neutrophilic asthma. Phosphodiesterase inhibitors, anti-TSLP antibodies, azithromycin, and anti-cholinergic agents are promising drugs for neutrophilic asthma. However, clinical research targeting neutrophilic inflammation is required to elucidate the optimal treatment.
Abnormal Uterine Bleeding in Perimenopausal Women: The Role of Hysteroscopy and Its Impact on Quality of Life and Sexuality
Authors: Vitale Salvatore Giovanni , Watrowski Rafał , Barra Fabio , D’Alterio Maurizio Nicola , Carugno Jose , Sathyapalan Thozhukat , Kahramanoglu Ilker , Reyes-Muñoz Enrique , Lin Li-Te , Urman Bulent , Ferrero Simone , Angioni Stefano
Published: May 2022

Abnormal uterine bleeding (AUB) is a frequent symptom in perimenopausal women. It is defined as uterine bleeding in which the duration, frequency, or amount of bleeding is considered excessive and negatively affects the woman’s quality of life (QoL) and psychological well-being. In cases of structural uterine pathology, hysterectomy (usually performed via a minimally invasive approach) offers definitive symptom relief and is associated with long-lasting improvement of QoL and sexuality. However, over the past 30 years, uterus-preserving treatments have been introduced as alternatives to hysterectomy. Hysteroscopic polypectomy, myomectomy, or endometrial resection/endometrial ablation are minimally invasive techniques that can be used as an alternative to hysterectomy to treat AUB due to benign conditions. Although associated with high patient satisfaction and short-term improvement in their QoL, hysteroscopic treatments do not eliminate the risk of AUB recurrence or the need for further intervention. Therefore, considering the impact of different treatment options on QoL and sexuality during preoperative shared decision making could help identify the most appropriate and personalized treatment options for perimenopausal women suffering from AUB.
The Role of SNHG15 in the Pathogenesis of Hepatocellular Carcinoma
Authors: Damaskos Christos , Garmpis Nikolaos , Dimitroulis Dimitrios , Garmpi Anna , Diamantis Evangelos , Sarantis Panagiotis , Georgakopoulou Vasiliki E. , Patsouras Alexandros , Despotidis Markos , Prevezanos Dionysios , Syllaios Athanasios , Marinos Georgios , Koustas Evangelos , Vallilas Christos , Antoniou Efstathios A. , Kontzoglou Konstantinos , Savvanis Spyridon , Kouraklis Gregory
Published: May 2022

Long non-coding RNAs (lncRNAs) are transcripts of more than 200 nucleotides which cannot be translated into proteins. Small nucleolar RNA host gene 15 (SNHG15) is a lncRNA whose dysregulation has been found to have an important impact on carcinogenesis and affect the prognosis of cancer patients in various cancer types. Hepatocellular carcinoma (HCC) is one of the most common cancers with a poor long-term prognosis, while the best prognostic factor of the disease is its early diagnosis and surgery. Consequently, the investigation of the mechanisms of hepatocarcinogenesis, as well as the discovery of efficient molecular markers and therapeutic targets are of great significance. An extensive literature search was performed in MEDLINE in order to identify clinical studies that tried to reveal the role of SNHG15 in HCC. We used keywords such as ‘HCC’, ‘hepatocellular carcinoma’, ‘SNHG15’ and ‘clinical study’. Finally, we included four studies written in English, published during the period 2016–2021. It was revealed that SNHG15 is related to the appearance of HCC via different routes and its over-expression affects the overall survival of the patients. More assays are required in order to clarify the potential role of SNHG15 as a prognostic tool and therapeutic target in HCC.
Important Risk Factors in Patients with Nonvalvular Atrial Fibrillation Taking Dabigatran Using Integrated Machine Learning Scheme—A Post Hoc Analysis
Authors: Huang Yung-Chuan , Cheng Yu-Chen , Jhou Mao-Jhen , Chen Mingchih , Lu Chi-Jie
Published: May 2022

Our study aims to develop an effective integrated machine learning (ML) scheme to predict vascular events and bleeding in patients with nonvalvular atrial fibrillation taking dabigatran and identify important risk factors. This study is a post-hoc analysis from the Randomized Evaluation of Long-Term Anticoagulant Therapy trial database. One traditional prediction method, logistic regression (LGR), and four ML techniques—naive Bayes, random forest (RF), classification and regression tree, and extreme gradient boosting (XGBoost)—were combined to construct our scheme. Area under the receiver operating characteristic curve (AUC) of RF (0.780) and XGBoost (0.717) was higher than that of LGR (0.674) in predicting vascular events. In predicting bleeding, AUC of RF (0.684) and XGBoost (0.618) showed higher values than those generated by LGR (0.605). Our integrated ML feature selection scheme based on the two convincing prediction techniques identified age, history of congestive heart failure and myocardial infarction, smoking, kidney function, and body mass index as major variables of vascular events; age, kidney function, smoking, bleeding history, concomitant use of specific drugs, and dabigatran dosage as major variables of bleeding. ML is an effective data analysis algorithm for solving complex medical data. Our results may provide preliminary direction for precision medicine.
Personalized Model to Predict Small for Gestational Age at Delivery Using Fetal Biometrics, Maternal Characteristics, and Pregnancy Biomarkers: A Retrospective Cohort Study of Births Assisted at a Spanish Hospital
Authors: Dieste-Pérez Peña , Savirón-Cornudella Ricardo , Tajada-Duaso Mauricio , Pérez-López Faustino R. , Castán-Mateo Sergio , Sanz Gerardo , Esteban Luis Mariano
Published: May 2022

Small for gestational age (SGA) is defined as a newborn with a birth weight for gestational age < 10th percentile. Routine third-trimester ultrasound screening for fetal growth assessment has detection rates (DR) from 50 to 80%. For this reason, the addition of other markers is being studied, such as maternal characteristics, biochemical values, and biophysical models, in order to create personalized combinations that can increase the predictive capacity of the ultrasound. With this purpose, this retrospective cohort study of 12,912 cases aims to compare the potential value of third-trimester screening, based on estimated weight percentile (EPW), by universal ultrasound at 35–37 weeks of gestation, with a combined model integrating maternal characteristics and biochemical markers (PAPP-A and β-HCG) for the prediction of SGA newborns. We observed that DR improved from 58.9% with the EW alone to 63.5% with the predictive model. Moreover, the AUC for the multivariate model was 0.882 (0.873–0.891 95% C.I.), showing a statistically significant difference with EPW alone (AUC 0.864 (95% C.I.: 0.854–0.873)). Although the improvements were modest, contingent detection models appear to be more sensitive than third-trimester ultrasound alone at predicting SGA at delivery.
Land Cover Classification from Hyperspectral Images via Local Nearest Neighbor Collaborative Representation with Tikhonov Regularization
Authors: Yang Rongchao , Zhou Qingbo , Fan Beilei , Wang Yuting
Published: May 2022

The accurate and timely monitoring of land cover types is of great significance for the scientific planning, rational utilization, effective protection and management of land resources. In recent years, land cover classification based on hyperspectral images and the collaborative representation (CR) model has become a hot topic in the field of remote sensing. However, most of the existing CR models do not consider the problem of sample imbalance, which affects the classification performance of CR models. In addition, the Tikhonov regularization term can improve the classification performance of CR models, but greatly increases the computational complexity of CR models. To address the above problems, a local nearest neighbor (LNN) method is proposed in this paper to select the same number of nearest neighbor samples from each nearest class of the test sample to construct a dictionary. This is then introduced into the original collaborative representation classification (CRC) method and CRC with Tikhonov regularization (CRT) for land cover classification, denoted as LNNCRC and LNNCRT, respectively. To verify the effectiveness of the proposed LNNCRC and LNNCRT methods, the classification performance and running time of the proposed methods are compared with those of six popular CR models on a hyperspectral scene with nine land cover types. The experimental results show that the proposed LNNCRT method achieves the best land cover classification performance, and the proposed LNNCRC and LNNCRT methods not only further exclude the interference of irrelevant training samples and classes, but also effectively eliminate the influence of imbalanced training samples, so as to improve the classification performance of CR models and effectively reduce the computational complexity of CR models.
Ecological Security and Ecosystem Quality: A Case Study of Xia-Zhang-Quan Metropolitan Area in China
Authors: Liang Fachao , Bai Mengdi , Hu Qiyu , Lin Sheng-Hau
Published: May 2022

Ecological security patterns are an effective tool by which to balance economic development with ecological protection. This study used the Integrated Valuation of Ecosystem Services and Tradeoffs (InVEST) model in conjunction with circuit theory to evaluate ecosystem quality from four dimensions: background conditions, topography, landscape structure, and ecological resistance. Our objective was to identify ecological “pinch points” in order to create an ecological security pattern that would be sustainable under a range of land use functions. We selected as the research target the Xia-Zhang-Quan metropolitan area in China due to the extensive soil erosion and general degradation of ecosystems caused by its rapid socio-economic development. Our analysis identified 17 ecological source sites covering 11,512 km2, which accounts for 45.36% of the total area. The inter-source corridor includes 31 key corridors and 10 potential corridors covering 3305 km. The average ecosystem quality of this area was estimated at 0.65 at an optimal granularity of 400 m. The distribution of resistance values in the study area was relatively concentrated with the land divided into an ecological buffer zone (34.6%), an environmentally sensitive zone (10.9%), and a blocking zone (9.2%). Our analysis also revealed various corridors based on the regional and functional characteristics of ecological elements and improvement strategies at the pinch points to help restore the function of ecological sources. Protection of these corridors will help to maintain regional ecological security patterns and optimize the structure of ecological spaces with the aim of achieving sustainable development.