bluuebunny/crossref_metadata_2025 huggingface dataset:
Detection of COVID-19 epidemic outbreak using machine learning
Authors: Cho Giphil , Park Jeong Rye , Choi Yongin , Ahn Hyeonjeong , Lee Hyojung
Published: December 2023

Background

The coronavirus disease (COVID-19) pandemic has spread rapidly across the world, creating an urgent need for predictive models that can help healthcare providers prepare and respond to outbreaks more quickly and effectively, and ultimately improve patient care. Early detection and warning systems are crucial for preventing and controlling epidemic spread.

Objective

In this study, we aimed to propose a machine learning-based method to predict the transmission trend of COVID-19 and a new approach to detect the start time of new outbreaks by analyzing epidemiological data.

Methods

We developed a risk index to measure the change in the transmission trend. We applied machine learning (ML) techniques to predict COVID-19 transmission trends, categorized into three labels: decrease (L0), maintain (L1), and increase (L2). We used Support Vector Machine (SVM), Random Forest (RF), and XGBoost (XGB) as ML models. We employed grid search methods to determine the optimal hyperparameters for these three models. We proposed a new method to detect the start time of new outbreaks based on label 2, which was sustained for at least 14 days (i.e., the duration of maintenance). We compared the performance of different ML models to identify the most accurate approach for outbreak detection. We conducted sensitivity analysis for the duration of maintenance between 7 days and 28 days.

Results

ML methods demonstrated high accuracy (over 94%) in estimating the classification of the transmission trends. Our proposed method successfully predicted the start time of new outbreaks, enabling us to detect a total of seven estimated outbreaks, while there were five reported outbreaks between March 2020 and October 2022 in Korea. It means that our method could detect minor outbreaks. Among the ML models, the RF and XGB classifiers exhibited the highest accuracy in outbreak detection.

Conclusion

The study highlights the strength of our method in accurately predicting the timing of an outbreak using an interpretable and explainable approach. It could provide a standard for predicting the start time of new outbreaks and detecting future transmission trends. This method can contribute to the development of targeted prevention and control measures and enhance resource management during the pandemic.
The comparison between effects of Taichi and conventional exercise on functional mobility and balance in healthy older adults: a systematic literature review and meta-analysis
Authors: Li Yiting , Liu Meng , Zhou Kaixiang , Dong Gengxin , Manor Brad , Bao Dapeng , Zhou Junhong
Published: December 2023

Background

Taichi is beneficial for functional mobility and balance in older adults. However, such benefits of Taichi when comparing to conventional exercise (CE) are not well understood due to large variance in study protocols and observations.

Methods

We reviewed publications in five databases. Eligible studies that examined the effects of Taichi on the outcomes of functional mobility and balance in healthy older adults as compared to CE were included. Subgroup analyses compared the effects of different types of CE (e.g., single and multiple-type exercise) and different intervention designs (e.g., Taichi types) on those outcomes (Registration number: CRD42022331956).

Results

Twelve studies consisting of 2,901 participants were included. Generally, compared to CE, Taichi induced greater improvements in the performance of Timed-Up-and-Go (SMD = −0.18, [−0.33 to −0.03], p = 0.040, I2 = 59.57%), 50-foot walking (MD = −1.84 s, [−2.62 to −1.07], p < 0.001, I2 = 0%), one-leg stance with eyes open (MD = 6.00s, [2.97 to 9.02], p < 0.001, I2 = 83.19%), one-leg stance with eyes closed (MD = 1.65 s, [1.35 to 1.96], p < 0.001, I2 = 36.2%), and functional reach (SMD = 0.7, [0.32 to 1.08], p < 0.001, I2 = 86.79%) tests. Subgroup analyses revealed that Taichi with relatively short duration (<20 weeks), low total time (≤24 h), and/or using Yang-style, can induce significantly greater benefits for functional mobility and balance as compared to CE. Uniquely, Taichi only induced significantly greater improvements in Timed-Up-and-Go compared to single- (SMD = −0.40, [−0.55 to −0.24], p < 0.001, I2 = 6.14%), but not multiple-type exercise. A significant difference between the effects of Taichi was observed on the performance of one-leg stance with eyes open when compared to CE without balance (MD = 3.63 s, [1.02 to 6.24], p = 0.006, I2 = 74.93%) and CE with balance (MD = 13.90s, [10.32 to 17.48], p < 0.001, I2 = 6.1%). No other significant difference was shown between the influences of different CE types on the observations.

Conclusion

Taichi can induce greater improvement in functional mobility and balance in older adults compared to CE in a more efficient fashion, especially compared to single-type CE. Future studies with more rigorous design are needed to confirm the observations here.
Public first aid education model design study based on user experience
Authors: Luo Jing , Zheng Kaiqiao , Hong Wudi
Published: December 2023

Background

Presently, China’s first aid penetration rate remains relatively low, leaving ample room for improvement in the existing first aid education model. Given its role as a service for the general public, public first aid education must thoroughly consider the learning needs and experiences of the public when designing the teaching mode.

Methods

Semi-structured interviews were employed to gather detailed insights into participants’ experiences in the first aid learning process. Subsequently, NVivo was utilized to analyze the interviews and identify specific design strategies. Additionally, a 7-point scale questionnaire was employed to assess the intervention effects of music familiarity and the simulation of teaching aids on users’ willingness and confidence in learning. Building upon the design strategy, a “feedback device + app” approach was proposed. Finally, user satisfaction was evaluated through a scale questionnaire.

Results

The use of familiar music had a significant positive effect on participants’ willingness and confidence to learn, while users’ fear of teaching aids had no effect on willingness and confidence. The user experience-based first aid education model can better meet the public’s learning needs for first aid knowledge and skills.

Conclusion

This study proposes a first aid education model based on user experience design methodology, which optimizes the public’s self-learning experience by evoking positive emotions while circumventing negative emotions. The educational model was recognized by users in terms of design concepts and is expected to help increase first aid prevalence in the future.
Turnover intention of nurses in public hospitals and its association with quality of working life: a cross-sectional survey in six provinces in China
Authors: Tang Changmin , Zhou Sitong , Liu Chaojie , Min Rui , Cai Ruipeng , Lin Taoyu
Published: December 2023

Objectives

High turnover intention can exacerbate the workforce shortage of nurses. This study aimed to determine the level of turnover intention of public hospital nurses in China and its associated factors.

Methods

A cross-sectional questionnaire survey of 2,863 nurses was conducted in 48 public hospitals across six provinces in mainland China, measuring the sociodemographic (gender, age, marital status, and monthly basic salary) and work characteristics (professional title, workload, night sleep deprivation, and workplace violence) of respondents, their quality of working life (QWL), and turnover intention. Multivariate logistic regression models were established to determine the association between QWL and turnover intention after adjustment for variations of the sociodemographic and work characteristics.

Results

Overall, 42.8% of respondents reported turnover intention. Higher QWL scores (AOR = 0.824 for job and career satisfaction, p < 0.001; AOR = 0.894 for professional pride, p < 0.001; AOR = 0.911 for balance between work and family, p < 0.05) were associated with lower turnover intention. Workplace violence was the strongest predictor of higher turnover intention (AOR = 3.003–4.767) amongst the sociodemographic and work characteristics, followed by an age between 30 and 40 years (AOR = 1.457 relative to <30 years), and night sleep deprivation (AOR = 1.391–1.808). Senior professional title had a protective effect (AOR = 0.417 relative to no title) on turnover intention.

Conclusion

High levels of turnover intention are evident across China in nurses employed by public hospitals, in particular in those aged between 30 and 40 years. Low QWL and poor work environment are significant predictors of turnover intention.
A reflection on COVID-19 and oral mucosal lesion: a systematic review
Authors: Kot Wai Ying , Li Jing Wen , Chan Alice Kit Ying , Zheng Li Wu
Published: December 2023

Introduction

The aim of this systematic review is to provide a clinical update of the current knowledge on COVID-19 and oral mucosal lesions, to analyze the types and prevalence of oral mucosal lesions in patients with COVID-19, and to clarify the potential association between COVID-19 and oral mucosal lesions.

Methods

The literature search was conducted using PubMed, Web of Science, Scopus and the Cochrane Library, as well as literatures via manual searches of the reference lists of included studies. Studies published in English that mentioned oral mucosal lesions in patients with COVID-19 were included, resulting in a total of 31 studies.

Results

Most of the included studies were considered to have a moderate to high risk of bias according to the Joanna Briggs Institute bias assessment tools. Based on COVID-19 severity, the characteristics and patterns of oral mucosal lesions in COVID-19 patients were described, analyzed and synthesized. Overall, ulcers without specific diagnosis had the highest prevalence in COVID-19 patients, followed by traumatic ulcers, candidiasis, petechiae and aphthous-like lesions. Homogeneity of data cannot be achieved in statical analysis, indicating randomness of outcome (ulcers without specific diagnosis, 95% CI: 28%–96%, I2 = 98.7%).

Discussion

Given the limited evidence from currently available studies, the association between COVID-19 and oral mucosal lesions remains difficult to clarify. Healthcare professionals should be aware of the possible association between COVID-19 and oral mucosal lesions, and we hereby discuss our findings.
Effect of sorbitan ester structure on the separation between tetrahydrofuran and water
Authors: Bartokova Bibiana , Marangoni Alejandro G. , Laredo Thamara , Pensini Erica
Published: December 2023

This study separates tetrahydrofuran (THF)-water mixtures containing varying THF percentages, using sorbitan esters (Spans) with different tail characteristics. We probe the separation mechanisms using attenuated total reflectance-Fourier transform infrared spectroscopy and small angle X ray scattering (SAXS). THF and water are miscible and interact through hydrogen bonds. Water splits the COC absorbance band of THF into a peak at ≈1,070 cm−1 (crystalline THF) and a dominant peak at ≈1,050 cm−1 (glassy THF), indicating disorder. Depending on the Span, separation occurs for mixtures containing up to 70% THF (v/v, relative to water). Spans with unsaturated tails separate the lowest THF percentages. Tail length and number of Span tails enhances ordering of THF, and the crystalline THF peak at ≈1,070 cm−1 dominates. Spans interact with THF through hydrogen bonds, as reflected in the splitting of the COC band of THF. Furthermore, C-H…O hydrogen bonds cause a blueshift in the νas(CH2) band of Spans with increasing THF. This effect is most significant in Span 40 and 60, indicating that they interact with THF more strongly than Span 20, Span 80 and Span 85. In contrast, they interact with water less strongly than Span 20, Span 80 and Span 85, as indicated by their flocculation at low THF percentages. Therefore, we propose that separation between THF and water occurs primarily through two mechanisms: 1) Span 20, Span 80 and Span 85 compete against THF for interactions with water through their hydrophilic head, and 2) Span 40 and Span 60 preferentially interact with THF through their tails. Nonetheless, water also interacts with the heads of Span 40 and Span 60, as indicated by SAXS. SAXS shows that in THF Spans self-assemble into micelles, which aggregate into either surface fractals or mass fractals. There are two persistence lengths because of the limited order in THF. Water orders self-assembled structures, likely by favoring the formation of micelles which host water in their interior. Therefore, we identify a single persistence length (≈25 Å), representative of the distance between the micelle centers.
Ultra-low-mass and small-radius white dwarfs made of heavy elements
Authors: Xia Cheng-Jun , Huang Yong-Feng , Li Hong-Bo , Shao Lijing , Xu Ren-Xin
Published: December 2023

Seven possible ultra-low-mass and small-radius white dwarfs have been recently identified, with masses ranging from ∼0.02 M⊙ to ∼0.08 M⊙ and radii ranging from ∼ 4,270 km to 10670 km. The mass–radius measurements of these white dwarfs pose challenges to traditional white dwarf models, assuming they are mostly made of nuclei lighter than 56Fe. In this work, we consider the possibility that those white dwarfs are made of heavier elements. Due to the small charge-to-mass ratios in heavy elements, the electron number density in white dwarf matter is effectively reduced, which reduces the pressure with additional contributions of lattice energy and electron polarization corrections. This consequently leads to white dwarfs with much smaller masses and radii, which coincide with the seven ultra-low-mass and small-radius white dwarfs. The mass of the most massive white dwarfs is effectively reduced and could possibly account for the sub-Chandrasekhar progenitors in underluminous Type Ia supernovae. The corresponding equation of state and matter contents of dense stellar matter with and without reaching the cold-catalyzed ground state are presented, which are obtained using the latest Atomic Mass Evaluation (AME 2020). Further observations are necessary to unveil the actual matter contents in those white dwarfs via, e.g., spectroscopy, asteroseismology, and the discoveries of other ultra-low-mass and small-radius white dwarfs.
A widespread picornavirus affects the hemocytes of the noble pen shell (Pinna nobilis), leading to its immunosuppression
Authors: Carella Francesca , Prado Patricia , De Vico Gionata , Palić Dušan , Villari Grazia , García-March José Rafael , Tena-Medialdea José , Cortés Melendreras Emilio , Giménez-Casalduero Francisca , Sigovini Marco , Aceto Serena
Published: December 2023

Introduction

The widespread mass mortality of the noble pen shell (Pinna nobilis) has occurred in several Mediterranean countries in the past 7 years. Single-stranded RNA viruses affecting immune cells and leading to immune dysfunction have been widely reported in human and animal species. Here, we present data linking P. nobilis mass mortality events (MMEs) to hemocyte picornavirus (PV) infection. This study was performed on specimens from wild and captive populations.

Methods

We sampled P. nobilis from two regions of Spain [Catalonia (24 animals) and Murcia (four animals)] and one region in Italy [Venice (6 animals)]. Each of them were analyzed using transmission electron microscopy (TEM) to describe the morphology and self-assembly of virions. Illumina sequencing coupled to qPCR was performed to describe the identified virus and part of its genome.

Results and discussion

In 100% of our samples, ultrastructure revealed the presence of a virus (20 nm diameter) capable of replicating within granulocytes and hyalinocytes, leading to the accumulation of complex vesicles of different dimensions within the cytoplasm. As the PV infection progressed, dead hemocytes, infectious exosomes, and budding of extracellular vesicles were visible, along with endocytic vesicles entering other cells. The THC (total hemocyte count) values observed in both captive (eight animals) (3.5 × 104–1.60 × 105 ml−1 cells) and wild animals (14 samples) (1.90–2.42 × 105 ml−1 cells) were lower than those reported before MMEs. Sequencing of P. nobilis (six animals) hemocyte cDNA libraries revealed the presence of two main sequences of Picornavirales, family Marnaviridae. The highest number of reads belonged to animals that exhibited active replication phases and abundant viral particles from transmission electron microscopy (TEM) observations. These sequences correspond to the genus Sogarnavirus—a picornavirus identified in the marine diatom Chaetoceros tenuissimus (named C. tenuissimus RNA virus type II). Real-time PCR performed on the two most abundant RNA viruses previously identified by in silico analysis revealed positive results only for sequences similar to the C. tenuissimus RNA virus. These results may not conclusively identify picornavirus in noble pen shell hemocytes; therefore, further study is required. Our findings suggest that picornavirus infection likely causes immunosuppression, making individuals prone to opportunistic infections, which is a potential cause for the MMEs observed in the Mediterranean.
Functional identification of PGM1 in the regulating development and depositing of inosine monophosphate specific for myoblasts
Authors: Zhao Wei , Cai Zhengyun , Wei Chuanhao , Ma Xiaoping , Yu Baojun , Fu Xi , Zhang Tong , Gu Yaling , Zhang Juan
Published: December 2023

Background

Inosine monophosphate (IMP) is naturally present in poultry muscle and plays a key role in improving meat flavour. However, IMP deposition is regulated by numerous genes and complex molecular networks. In order to excavate key candidate genes that may regulate IMP synthesis, we performed proteome and metabolome analyses on the leg muscle, compared to the breast muscle control of 180-day-old Jingyuan chickens (hens), which had different IMP content. The key candidate genes identified by a differential analysis were verified to be associated with regulation of IMP-specific deposition.

Results

The results showed that the differentially expressed (DE) proteins and metabolites jointly involve 14 metabolic pathways, among which the purine metabolic pathway closely related to IMP synthesis and metabolism is enriched with four DE proteins downregulated (with higher expression in breast muscles than in leg muscles), including adenylate kinase 1 (AK1), adenosine monophosphate deaminase 1 (AMPD1), pyruvate kinase muscle isoenzyme 2 (PKM2) and phosphoglucomutase 1 (PGM1), six DE metabolites, Hypoxanthine, Guanosine, L-Glutamine, AICAR, AMP and Adenylsuccinic acid. Analysis of PGM1 gene showed that the high expression of PGM1 promoted the proliferation and differentiation of myoblasts and inhibited the apoptosis of myoblasts. ELISA tests have shown that PGM1 reduced adenosine triphosphate (ATP) and IMP and uric acid (UA), while enhancing the biosynthesis of hypoxanthine (HX). In addition, up-regulation of PGM1 inhibited the expression of purine metabolism pathway related genes, and promoted the IMP de novo and salvage synthesis pathways.

Conclusion

This study preliminarily explored the mechanism of action of PGM1 in regulating the growth and development of myoblasts and specific IMP deposition in Jingyuan chickens, which provided certain theoretical basis for the development and utilization of excellent traits in Jingyuan chickens.
Diffusion tensor-based analysis of white matter in dogs with idiopathic epilepsy
Authors: Beckmann Katrin M. , Wang-Leandro Adriano , Steffen Frank , Richter Henning , Dennler Matthias , Bektas Rima , Carrera Ines , Haller Sven
Published: December 2023

Introduction

The understanding of epileptic seizure pathogenesis has evolved over time, and it is now generally accepted that not only are cortical and subcortical areas involved but also the connection of these regions in the white matter (WM). Recent human neuroimaging studies confirmed the involvement of the WM in several epilepsy syndromes. Neuroimaging studies investigating WM integrity with diffusion tensor imaging (DTI) in canine idiopathic epilepsy are lacking. This study aimed to test the hypothesis that WM diffusion changes can be found in dogs affected by idiopathic epilepsy.

Method

Twenty-six dogs with idiopathic epilepsy (15 Border Collies and 11 Greater Swiss Mountain dogs) and 24 healthy controls (11 Beagle dogs, 5 Border Collies, and 8 Greater Swiss Mountain dogs) were prospectively enrolled. Most dogs with idiopathic epilepsy (17/26) were enrolled within 3 months after seizure onset. Diffusion tensor imaging of the brain with 32 diffusion directions (low b value = 0 s/mm2; maximal b value = 800 s/mm2) was performed in a 3 Tesla scanner. Tract-based spatial statistics (TBSS), a voxel-based approach, was used to investigate changes in fractional anisotropy (FA) and mean diffusivity (MD) in the idiopathic epilepsy group compared to the healthy control group. Additionally, FA and MD were investigated in the region of corpus callosum and cingulate white matter in both groups.

Results

We observed subtle changes in WM DTI between the idiopathic epilepsy group and the healthy control group limited to cingulate WM, with a significantly lower FA in the idiopathic epilepsy group compared to the healthy control group in the region of interest (ROI) approach (p = 0.027). No significant changes were found between the idiopathic epilepsy group and the healthy control group in the TBSS analysis and in the corpus callosum in the ROI approach.

Conclusion

This study supports the cingulate area as a target structure in canine epilepsy. The subtle changes only might be explained by the short duration of epilepsy, small sample sizes, and the higher variability in canine brain anatomy. Furthermore, all included dogs showed generalized tonic-clonic seizures, possibly affected by generalized epilepsy syndrome, which are also associated with less pronounced DTI changes in humans than focal epilepsy syndromes.
APOBEC3 family proteins as drivers of virus evolution
Authors: Jonathan Michael , Ikeda Terumasa
Published: December 2023

The apolipoprotein B mRNA editing enzyme catalytic polypeptide-like (APOBEC) family consists of cytosine deaminases implicated in diverse and important biological functions. APOBEC3 (A3) proteins belong to the APOBEC/AID family, and they catalyze the deamination of cytosine to uracil in single-stranded DNA and, to a lesser extent, in RNA substrates. In humans, seven A3 genes have been identified (A3A, A3B, A3C, A3D, A3F, A3G, and A3H). The introduction of lethal G-to-A or C-to-U mutations into certain viral genomes leads to virus inactivation. However, the mutagenic capability of A3 proteins could serve as a source of mutations to drive virus evolution. Therefore, recent studies have implied the role of A3 proteins in aiding the evolution of viruses, conferring them with severe manifestations such as drug resistance and/or immune evasion. In this review, we discuss in depth the interactions of A3 proteins with viruses that infect humans and our self-proteins.
Dentinogenesis imperfectában és hemofília A betegségben szenvedő tinédzser protetikai ellátása
Authors: Lipták Laura , Lipták Klaudia , Balaton Gergely , Rózsa Noémi Katinka
Published: December 2023

Bevezetés: A dentinogenesis imperfecta (DI) egy dentint érintő genetikai betegség. Az autoszomális dominánsan öröklődőrendellenesség csak a fogak kötőszöveti eredetű szöveteiben manifesztálódik, így a zománcban és a fogágybannincs változás. A szabálytalan dentin a fogak elszíneződését okozza, melyről a zománc könnyen leválhat. A DI-benszenvedő betegek fogászati kezelésében nagyon fontos a korai felismerés, így beavatkozhatunk mielőtt a fogazat súlyosállapotba kerülne.Esetismertetés: Egy 15 éves fiúbeteg érkezett a Gyermekfogászati és Fogszabályozási Klinikára az alsó metszőfogairólleesett direkt héjai, valamint minden fogát érintő esztétikai igénye miatt. A páciens anamnézisében jelezte hemofíliaA betegségét, illetve édesapja DI érintettségét. A kezelési tervben indirekt restaurátumokat terveztünk. A kopott fogállománymiatt harapásemelésre is szükség volt. Mindkét állcsontra végleges ellátásként monolitikus cirkónium-dioxid szólókoronákat, illetve síneket terveztünk.Összefoglalás: A DI-ben szenvedő betegek számára a korai diagnózis kulcsfontosságú. A tudományos bizonyítékokazt mutatják, hogy a kórkép esetén az indirekt restaurátumokat előnyben kell részesíteni a direkt restaurátumokkal szemben.Hemofília A beteg kezelése során szoros együttműködés szükséges a hematológusával.
A 4 mm Micro Servo Control System in Fiber Positioner
Authors: Guo Shaoxiong , Yang Yan , Zhai Chao
Published: December 2023

As multi-object spectrographs (MOSs) continue to evolve, a notable trend has emerged—the increasing accommodation of fiber positioners on ever-more compact focal planes. This progression has seen the traditional stepper motors being supplanted by more space-efficient miniature hollow-cup motors. A significant challenge faced in the employment of these 4 mm diameter motors is the absence of compatible angle sensors, resulting in reliance on open-loop control methods for positioning. Addressing this challenge, this paper introduces a novel miniature angle sensor designed specifically for 4 mm hollow-cup motors, and presents a newly formulated closed-loop control scheme, which leverages this sensor to achieve accurate positioning. This marks the first implementation of an angle closed-loop control system within a 4 mm miniature hollow-cup motor used in MOS fiber positioners. Experimental evidence suggests that this sensored closed-loop mode substantially improves upon the energy efficiency and precision of fiber positioner placement, compared with traditional open-loop stepper control methods. Furthermore, the integration of these microsensors mitigates collision risks during the concurrent operation of fiber positioners by deactivating the motor power supply to prevent potential damage to the system.
Vibration Suppression of a Flexible Beam Structure Coupled with Liquid Sloshing via ADP Control Based on FBG Strain Measurement
Authors: Kong Chunyang , Zhao Dangjun , Liang Buge
Published: December 2023

In this study, an adaptive dynamic programming (ADP) control strategy based on the strain measurement of a fiber Bragg grating (FGB) sensor array is proposed for the vibration suppression of a complicated flexible-sloshing coupled system, which usually exists in aerospace engineering, such as launch vehicles with a large amount of liquid propellant as well as a flexible beam structure. To simplify the flexible-sloshing coupled dynamics model, the equivalent spring-mass-damper (SMD) model of liquid sloshing is employed, and a finite-element method (FEM) dynamic model for the beam structure coupled with the liquid sloshing is mathematically established. Then, a strain-based vibration dynamic model is derived by employing a transformation matrix based on the relationship between displacement and strain of the beam structure. To facilitate the design of a strain-based control, a tracking differentiator is designed to provide the strains’ derivative signals as partial states’ estimations. Feeding the system with the strain measurements and their derivatives’ estimations, an ADP controller with an action-dependent heuristic dynamic programming structure is proposed to suppress the vibration of the flexible-sloshing coupled system, and the corresponding Lyapunov stability of the closed-loop system is theoretically guaranteed. Numerical results show the proposed method can effectively suppress coupled vibration depending on limited strain measurements irrespective of external disturbances.
Solid–Liquid Separation and Its Environmental Impact on Manure Treatment in Scaled Pig Farms—Evidence Based on Life Cycle Assessment
Authors: Zhang Yijia , Bo Qinqing , Ma Xintian , Du Yating , Du Xinyi , Xu Liyang , Yang Yadong
Published: December 2023

Recently, there has been a significant focus on the issue of pollution caused by livestock and poultry rearing, which is recognized as a prominent contributor to nonpoint source pollution in the agricultural sector. This study employed the life cycle assessment (LCA) methodology to evaluate the environmental impact of several pig manure processing scenarios with the aim of determining the appropriate solid–liquid separation tool for large-scale pig farms. The findings indicate that the utilization of a screw extruder for solid–liquid separation in Scenario 2 has a lower environmental impact. In contrast to Scenario 1, Scenario 2 exhibits reduced environmental potential in the areas of global warming, human toxicity, acidification, and eutrophication. Specifically, the global warming, human toxicity, acidification, and eutrophication impacts decreased by 56%, 81%, 83%, and 273%, respectively, due to the implementation of solid–liquid separation. The type of solid–liquid separation equipment used during the processing of swine manure, as well as the subsequent treatment, have a significant impact on environmental emissions. Compared to Scenario 2, Scenario 3, which utilizes a centrifugal microfilter for solid–liquid separation, exhibits a lower environmental impact in terms of human toxicity, resulting in a reduction of 0.736 kg DCB-eq. In general, solid–liquid separation is a viable and environmentally friendly method for the disposal of waste from large-scale pig farms. The adoption of this method is highly recommended. During its implementation, careful consideration should be given to factors such as separation efficiency and pollution emissions. It is crucial to select appropriate equipment for solid–liquid separation to effectively process the waste.
Effect of Varied Salinity on Marigold Flowers: Reduced Size and Quantity Despite Enhanced Antioxidant Activity
Authors: Guzman María Rita , Marques Isabel
Published: December 2023

Salinity is an increasing problem worldwide that limits crop production. The cultivation of salt-tolerant flowers is a potential sustainable strategy for the exploitation of saline soils while avoiding the use of freshwater resources. However, our understanding of how flowers can adapt to salinity is still limited. In this study, we investigated the effects of salinity on a widely-used ornamental plant (Tagetes patula) by submitting three cultivars (cv. Aurora Orange, Fireball, Safari Scarlet) to different salinity levels (0, 50, 100, 300 mM). The plants were grown under controlled conditions and followed over two weeks. We tested the effect of salinity on plant growth and flower production, as well as on the levels of total carotenoids, polyphenols, and flavonoids, and the activities of antioxidant enzymes (catalase, peroxidase activity, ascorbate peroxidase). The findings indicate a substantial decline in both plant growth and flower production under saline conditions. Overall, plant height was ¼ lower, and there were 1/3 less flowers under salinity. Additionally, there was a consistent rise in antioxidant compounds, highlighting the swift response of defense mechanisms, both enzymatic and non-enzymatic, to combat stress. The high levels of antioxidant compounds sustain the use of marigold flowers as a new source of nutritional compounds, with enriched nutritional contents. Yet, cultivation of these plants in saline conditions should carefully consider the pronounced adverse effects observed at high salinity levels (e.g., 100 and 300 mM) on both plant and flower production.
Knowledge and Perceptions of Final-Year Nursing Students Regarding Antimicrobials, Antimicrobial Resistance, and Antimicrobial Stewardship in South Africa: Findings and Implications to Reduce Resistance
Authors: Teague Elisma , Bezuidenhout Selente , Meyer Johanna C. , Godman Brian , Engler Deirdré
Published: December 2023

Antimicrobial resistance (AMR) is being increasingly seen as the next pandemic due to high morbidity and mortality rates, with Sub-Saharan Africa currently having the highest mortality rates driven by high rates of inappropriate prescribing in ambulatory care. In South Africa, nurses typically provide a range of services, including prescribing, in public ambulatory care clinics. However, little is currently known about the perception of final-year nursing students regarding antibiotic use, AMR, and antimicrobial stewardship (AMS). Consequently, we sought to address this important evidence gap. A quantitative descriptive study using a self-administered online questionnaire via Google Forms® was undertaken among six universities in South Africa offering a Baccalaureus of Nursing. Knowledge on the classes of antibiotics, organisms covered, and mechanism of action was lacking. The sample size to achieve a confidence interval of 95% with a 5% error margin was 174, increased to 200 to compensate for possible attrition. Only 15.3% of nurses knew that ceftazidime is not a fourth-generation cephalosporin, and only 16.1% knew that clavulanic acid does not decrease inflammation at the site of infection. In addition, only 58.9% and 67.7% agreed that the prescribing of broad-spectrum antibiotics and poor infection control, respectively, increase AMR. AMS was also not a well-known concept among final-year nurses. The lack of knowledge regarding antibiotics, AMR, and AMS among final-year nurses could have important repercussions in practice once these nurses are qualified. Consequently, this information gap needs to be urgently addressed going forward with updated curricula and post-qualification educational activities to reduce AMR in South Africa
Reactive Oxygen Species Damage Bovine Endometrial Epithelial Cells via the Cytochrome C-mPTP Pathway
Authors: Song Pengjie , Sun Mingkun , Liu Chen , Liu Jianguo , Lin Pengfei , Chen Huatao , Zhou Dong , Tang Keqiong , Wang Aihua , Jin Yaping
Published: December 2023

After parturition, bovine endometrial epithelial cells (BEECs) undergo serious inflammation and imbalance between oxidation and antioxidation, which is widely acknowledged as a primary contributor to the development of endometritis in dairy cows. Nevertheless, the mechanism of oxidative stress-mediated inflammation and damage in bovine endometrial epithelial cells remains inadequately defined, particularly the molecular pathways associated with mitochondria-dependent apoptosis. Hence, the present study was designed to explore the mechanism responsible for mitochondrial dysfunction-induced BEEC damage. In vivo, the expressions of proapoptotic protein caspase 3 and cytochrome C were increased significantly in dairy uteri with endometritis. Similarly, the levels of proapoptotic protein caspase 3, BAX, and cytochrome C were markedly increased in H2O2-treated BEECs. Our findings revealed pronounced BEEC damage in dairy cows with endometritis, accompanied by heightened expression of cyto-C and caspase-3 both in vivo and in vitro. The reduction in apoptosis-related protein of BEECs due to oxidant injury was notably mitigated following N-acetyl-L-cysteine (NAC) treatment. Furthermore, mitochondrial vacuolation was significantly alleviated, and mitochondrial membrane potential returned to normal levels after the removal of ROS. Excessive ROS may be the main cause of mitochondrial dysfunction. Mitochondrial permeability transition pore (mPTP) blockade by cyclophilin D (CypD) knockdown with CSA significantly blocked the flow of cytochrome C (cyto-C) and Ca2+ to the cytoplasm from the mitochondria. Our results indicate that elevated ROS and persistent opening of the mPTP are the main causes of oxidative damage in BEECs. Collectively our results reveal a new mechanism involving ROS-mPTP signaling in oxidative damage to BEECs, which may be a potential avenue for the clinical treatment of bovine endometritis.
Exploring the Antioxidant Potential of Blackberry and Raspberry Leaves: Phytochemical Analysis, Scavenging Activity, and In Vitro Polyphenol Bioaccessibility
Authors: Varzaru Iulia , Oancea Alexandra Gabriela , Vlaicu Petru Alexandru , Saracila Mihaela , Untea Arabela Elena
Published: December 2023

The goal of this research was nutritional evaluation through the phytochemical analysis of blackberry and raspberry leaves, the screening of their biological activity (antioxidant capacity and inhibition of lipid peroxidation), and the investigation of the effect of in vitro gastrointestinal digestion (GID) of blackberry and raspberry leaves on the bioaccessibility of polyphenol subclasses. The concentrations of the analyzed liposoluble antioxidants were higher (p < 0.05) in blackberry leaves compared to raspberry leaves, while a significant (p < 0.05) higher content of water-soluble antioxidants was registered in raspberry leaves (with a total polyphenol content of 26.2 mg GAE/g DW of which flavonoids accounted for 10.6 mg/g DW). Blackberry leaves had the highest antioxidant capacity inhibition of the superoxide radicals (O2•−), while raspberry leaves registered the highest inhibition of hydroxyl radicals (•OH), suggesting a high biological potency in scavenging-free radicals under in vitro systems. The maximum inhibition percentage of lipid peroxidation was obtained for blackberry leaves (24.86% compared to 4.37% in raspberry leaves), suggesting its potential to limit oxidative reactions. Simulated in vitro digestion showed that hydroxybenzoic acids registered the highest bioaccessibility index in the intestinal phase of both types of leaves, with gallic acid being one of the most bioaccessible phenolics. The outcomes of this investigation reveal that the most significant release of phenolic compounds from blackberry and raspberry leaves occurs either during or after the gastric phase. Knowledge about the bioaccessibility and stability of polyphenol compounds during digestion can provide significant insights into the bioavailability of these molecules and the possible effectiveness of plant metabolites for human health.
Antioxidant and Anti-Inflammatory Effects of Garlic in Ischemic Stroke: Proposal of a New Mechanism of Protection through Regulation of Neuroplasticity
Authors: Bautista-Perez Sandra Monserrat , Silva-Islas Carlos Alfredo , Sandoval-Marquez Oscar Uriel , Toledo-Toledo Jesús , Bello-Martínez José Manuel , Barrera-Oviedo Diana , Maldonado Perla D.
Published: December 2023

Stroke represents one of the main causes of death and disability in the world; despite this, pharmacological therapies against stroke remain insufficient. Ischemic stroke is the leading etiology of stroke. Different molecular mechanisms, such as excitotoxicity, oxidative stress, and inflammation, participate in cell death and tissue damage. At a preclinical level, different garlic compounds have been evaluated against these mechanisms. Additionally, there is evidence supporting the participation of garlic compounds in other mechanisms that contribute to brain tissue recovery, such as neuroplasticity. After ischemia, neuroplasticity is activated to recover cognitive and motor function. Some garlic-derived compounds and preparations have shown the ability to promote neuroplasticity under physiological conditions and, more importantly, in cerebral damage models. This work describes damage/repair mechanisms and the importance of garlic as a source of antioxidant and anti-inflammatory agents against damage. Moreover, we examine the less-explored neurotrophic properties of garlic, culminating in proposals and observations based on our review of the available information. The aim of the present study is to propose that garlic compounds and preparations could contribute to the treatment of ischemic stroke through their neurotrophic effects.
Chemical Composition and Biological Properties of New Romanian Lavandula Species
Authors: Marchidan Ionuț Georgică , Ortan Alina , Marcu Spinu Simona , Avramescu Sorin Marius , Avram Ionela , Fierascu Radu Claudiu , Babeanu Narcisa
Published: December 2023

The aims of the present study were to evaluate for the first time the chemical composition and antioxidant, antibacterial, antifungal and antiproliferative potentials of the Romanian George 90 lavender species, as well as parental species, L. angustifolia and L. latifolia. The L. angustifolia, L. latifolia and George 90 essential oils were analyzed by GC-MS/MS and the L. angustifolia, L. latifolia and George 90 hydroalcoholic extracts were analyzed by HPLC-DAD. The antioxidant, antibacterial, antifungal and antiproliferative assays revealed that all the investigated species showed significant activities. The results highlighted the chemical composition and the promising biological potentials of the L. angustifolia, L. latifolia and George 90 lavender species, validating their ethnomedicinal value, which offers potential applications as natural drugs.
Physiological Response of Spotted Seabass (Lateolabrax maculatus) to Different Dietary Available Phosphorus Levels and Water Temperature: Changes in Growth, Lipid Metabolism, Antioxidant Status and Intestinal Microbiota
Authors: Guo Jiarong , Wang Ling , Song Kai , Lu Kangle , Li Xueshan , Zhang Chunxiao
Published: December 2023

A 10-week growth experiment was conducted to assess the physiological response of spotted seabass (Lateolabrax maculatus) raised at moderate (27 °C) and high temperatures (33 °C) to different dietary available phosphorus (P) levels. Five diets with available P levels of 0.35, 0.55, 0.71, 0.82 and 0.92% were formulated, respectively. A water temperature of 33 °C significantly decreased growth performance and feed utilization, and increased oxidative stress and lipid deposition of spotted seabass compared with 27 °C. A second-order polynomial regression analysis based on weight gain (WG) showed that the available P requirement of spotted seabass raised at 27 °C and 33 °C was 0.72% and 0.78%, respectively. The addition of 0.71–0.82% P to the diet improved the growth performance, feed utilization, and antioxidant capacity of spotted seabass and alleviated the excessive lipid deposition compared with the low-P diet (0.35% P). Moreover, the addition of 0.71–0.92% P to diets increased the diversity of intestinal microbiota and the relative abundance of Lactococcus lactis and decreased the relative abundance of Plesiomonas compared with the low-P diet. Thus, dietary supplementation with 0.71–0.82% P improved the growth performance, antioxidant capacity and microbial composition of spotted seabass, and alleviated the disturbance of lipid metabolism caused by high temperature or low-P diet.
Buspirone Enhances Cell Survival and Preserves Structural Integrity during Oxidative Injury to the Retinal Pigment Epithelium
Authors: Biswal Manas R. , Paulson Ryan J. , Vichare Riddhi , Lewin Alfred S.
Published: December 2023

Chronic oxidative stress impairs the normal functioning of the retinal pigment epithelium (RPE), leading to atrophy of this cell layer in cases of advance age-related macular degeneration (AMD). The purpose of our study was to determine if buspirone, a partial serotonin 1A (5-HT1A) receptor agonist, protected against oxidative stress-induced changes in the RPE. We exposed differentiated human ARPE-19 cells to paraquat to induce oxidative damage in culture, and utilized a mouse model with sodium iodate (NaIO3)-induced oxidative injury to evaluate the effect of buspirone. To investigate buspirone’s effect on protective gene expression, we performed RT–PCR. Cellular toxicities and junctional abnormalities due to paraquat induction in ARPE-19 cells and buspirone’s impact were assessed via WST-1 assays and ZO-1 immunostaining. We used spectral-domain optical coherence tomography (SD-OCT) and ZO-1 immunostaining of RPE/choroid for structural analysis. WST-1 assays showed dose-dependent protection of viability in buspirone-treated ARPE-19 cells in culture and preservation of RPE junctional integrity under oxidative stress conditions. In the NaIO3 model, daily intraperitoneal injection (i.p.) of buspirone (30 mg/kg) for 12 days improved the survival of photoreceptors compared to those of vehicle-treated eyes. ZO-1-stained RPE flat-mounts revealed the structural preservation of RPE from oxidative damage in buspirone-treated mice, as well as in buspirone-induced Nqo1, Cat, Sqstm1, Gstm1, and Sod2 genes in the RPE/choroid compared to untreated eyes. Since oxidative stress is implicated in the pathogenesis AMD, repurposing buspirone, which is currently approved for the treatment of anxiety, might be useful in treating or preventing dry AMD.
A Multistage Rigid-Affine-Deformable Network for Three-Dimensional Multimodal Medical Image Registration
Authors: Strittmatter Anika , Caroli Anna , Zöllner Frank G.
Published: December 2023

Multimodal image registration is an important component of medical image processing, allowing the integration of complementary information from various imaging modalities to improve clinical applications like diagnosis and treatment planning. We proposed a novel multistage neural network for three-dimensional multimodal medical image registration, which addresses the challenge of larger rigid deformations commonly present in medical images due to variations in patient positioning in different scanners and rigid anatomical structures. This multistage network combines rigid, affine and deformable transformations in three stages. The network was trained unsupervised with Mutual Information and Gradient L2 loss. We compared the results of our proposed multistage network with a rigid-affine-deformable registration with the classical registration method NiftyReg as a baseline and a multistage network, which combines affine and deformable transformation, as a benchmark. To evaluate the performance of the proposed multistage network, we used four three-dimensional multimodal in vivo datasets: three renal MR datasets consisting of T1-weighted and T2-weighted MR scans and one liver dataset containing CT and T1-weighted MR scans. Experimental results showed that combining rigid, affine and deformable transformations in a multistage network leads to registration results with a high structural similarity, overlap of the corresponding structures (Dice: 76.7 ± 12.5, 61.1 ± 14.0, 64.8 ± 16.2, 68.1 ± 24.6 for the four datasets) and a low level of image folding (|J| ≤ 0: less than or equal to 1.1%), resulting in a medical plausible registration result.
Influence of Open Differential Design on the Mass Reduction Function
Authors: Karakašić Mirko , Konjatić Pejo , Glavaš Hrvoje , Grgić Ivan
Published: December 2023

The transmission of power and motion in road vehicles with internal combustion engines is achieved by different design variants of differential transmissions. The open differential transmission (ODT) is installed to a greater extent in passenger cars with rear-wheel drive due to its simpler design. Due to its robustness, it is possible to reduce its mass. Reducing the mass of the ODT, as well as reducing the mass of the other design elements of the vehicle, contributes to reducing the overall mass of the vehicle and improves the energy efficiency of the vehicle. The paper develops and proposes an algorithm that combines the design of the ODT according to ISO 23509:2006, the numerical calculation of the design elements (ring gear and drive shaft with pinion) using the finite element method (FEM) and the numerical global–local model, the topological optimization method (TOM) and the results of the FEM analysis in determining the design parameters. In addition, the proposed algorithm uses the application of the response surface method (RSM) in the construction of a mathematical model. With the proposed mathematical model, the mathematical objective function of the ODT overall mass reduction describes the influence of the previously selected design parameters on the overall mass reduction of the ODT. The mathematical model is also used to analyze the partial influence of the design parameters on the objective functions of the partial mass reduction of the ring gear and pinion drive shaft. Using the R2 and root mean square error (RMSE), an accuracy check of the proposed mathematical model was performed. According to the proposed algorithm and mathematical model, the two mentioned design elements of the ODT were optimized. After optimization, the overall mass of the ODM was reduced by 16.5%.