bluuebunny/crossref_metadata_2025 huggingface dataset:
The Anti-Inflammatory Role of Bilirubin on “Two-Hit” Sepsis Animal Model
Authors: Tran Duc Tin , Jeong Yong Yeon , Kim Jeong Min , Bae Hong Bum , Son Sung Kuk , Kwak Sang Hyun
Published: November 2020

Introduction: Bilirubin is a product of the heme catabolism pathway, and it is excreted in bile and removed from the body through the urine. Bilirubin has potent antioxidant properties but also plays a role in anti-inflammation by protecting the body against endotoxin-induced lung inflammation, down-regulating the expression of adhesion molecules, and inhibiting the infiltration of inflammatory cells. Thus, bilirubin is a promising agent that could use in inflammation disease treatment. The application of bilirubin on the “two-hit” sepsis animal model has been, to date, unknown. Methods: we used lipopolysaccharide to induce initial insults in C57BL/6 mice. After 24 h, mice underwent cecal ligation and puncture to induce the “two-hit” sepsis model. Next, mice were administered 30 mg/kg bilirubin and we observed an improvement. Results: We observed that bilirubin inhibited the expression of pro-inflammatory cytokines, while the levels of anti-inflammatory cytokines were significantly augmented in the lung. Bilirubin improved the survival rate in the sepsis model. Furthermore, we suggest that bilirubin can modulate the accumulation of T-regulatory cells and myeloid-derived suppressor cells. Notably, bilirubin suppressed the activation and functions of T-cells. Conclusions: These results clarified that bilirubin might improve tissue injury in sepsis through anti-inflammatory mechanisms.
Therapeutic Strategies to Reduce the Toxicity of Misfolded Protein Oligomers
Authors: Kreiser Ryan P. , Wright Aidan K. , Block Natalie R. , Hollows Jared E. , Nguyen Lam T. , LeForte Kathleen , Mannini Benedetta , Vendruscolo Michele , Limbocker Ryan
Published: November 2020

The aberrant aggregation of proteins is implicated in the onset and pathogenesis of a wide range of neurodegenerative disorders, including Alzheimer’s and Parkinson’s diseases. Mounting evidence indicates that misfolded protein oligomers produced as intermediates in the aggregation process are potent neurotoxic agents in these diseases. Because of the transient and heterogeneous nature of these elusive aggregates, however, it has proven challenging to develop therapeutics that can effectively target them. Here, we review approaches aimed at reducing oligomer toxicity, including (1) modulating the oligomer populations (e.g., by altering the kinetics of aggregation by inhibiting, enhancing, or redirecting the process), (2) modulating the oligomer properties (e.g., through the size–hydrophobicity–toxicity relationship), (3) modulating the oligomer interactions (e.g., by protecting cell membranes by displacing oligomers), and (4) reducing oligomer toxicity by potentiating the protein homeostasis system. We analyze examples of these complementary approaches, which may lead to the development of compounds capable of preventing or treating neurodegenerative disorders associated with protein aggregation.
Peripheral Nerve Regeneration and Muscle Reinnervation
Authors: Gordon Tessa
Published: November 2020

Injured peripheral nerves but not central nerves have the capacity to regenerate and reinnervate their target organs. After the two most severe peripheral nerve injuries of six types, crush and transection injuries, nerve fibers distal to the injury site undergo Wallerian degeneration. The denervated Schwann cells (SCs) proliferate, elongate and line the endoneurial tubes to guide and support regenerating axons. The axons emerge from the stump of the viable nerve attached to the neuronal soma. The SCs downregulate myelin-associated genes and concurrently, upregulate growth-associated genes that include neurotrophic factors as do the injured neurons. However, the gene expression is transient and progressively fails to support axon regeneration within the SC-containing endoneurial tubes. Moreover, despite some preference of regenerating motor and sensory axons to “find” their appropriate pathways, the axons fail to enter their original endoneurial tubes and to reinnervate original target organs, obstacles to functional recovery that confront nerve surgeons. Several surgical manipulations in clinical use, including nerve and tendon transfers, the potential for brief low-frequency electrical stimulation proximal to nerve repair, and local FK506 application to accelerate axon outgrowth, are encouraging as is the continuing research to elucidate the molecular basis of nerve regeneration.
The Anti-Neuroinflammatory Role of Anthocyanins and Their Metabolites for the Prevention and Treatment of Brain Disorders
Authors: Henriques Joana F. , Serra Diana , Dinis Teresa C. P. , Almeida Leonor M.
Published: November 2020

Anthocyanins are naturally occurring polyphenols commonly found in fruits and vegetables. Numerous studies have described that anthocyanin-rich foods may play a crucial role in the prevention and treatment of different pathological conditions, which have encouraged their consumption around the world. Anthocyanins exhibit a significant neuroprotective role, mainly due to their well-recognized antioxidant and anti-inflammatory properties. Neuroinflammation is an intricate process relevant in both homeostatic and pathological circumstances. Since the progression of several neurological disorders relies on neuroinflammatory process, targeting brain inflammation has been considered a promising strategy in those conditions. Recent data have shown the anti-neuroinflammatory abilities of many anthocyanins and of their metabolites in the onset and development of several neurological disorders. In this review, it will be discussed the importance and the applicability of these polyphenolic compounds as neuroprotective agents and it will be also scrutinized the molecular mechanisms underlying the modulation of neuroinflammation by these natural compounds in the context of several brain diseases.
Gene Modified CAR-T Cellular Therapy for Hematologic Malignancies
Authors: Lin Wen-Ying , Wang Hsin-Hui , Chen Yi-Wei , Lin Chun-Fu , Fan Hueng-Chuen , Lee Yi-Yen
Published: November 2020

With advances in the understanding of characteristics of molecules, specific antigens on the surface of hematological malignant cells were identified and multiple therapies targeting these antigens as neoplasm treatments were developed. Among them, chimeric antigen receptor (CAR) T-cell therapy, which got United States Food and Drug Administration (FDA) approval for relapsed/refractory (r/r) diffuse large B-cell lymphoma (DLBCL) as well as for recurrent acute lymphoblastic leukemia (ALL) within the past five years, and for r/r mantle cell lymphoma (MCL) this year, represents one of the most rapidly evolving immunotherapies. Nevertheless, its applicability to other hematological malignancies, as well as its efficacy and persistence are fraught with clinical challenges. Currently, more than one thousand clinical trials in CAR T-cell therapy are ongoing and its development is changing rapidly. This review introduces the current status of CAR T-cell therapy in terms of the basic molecular aspects of CAR T-cell therapy, its application in hematological malignancies, adverse reactions during clinical use, remaining challenges, and future utilization.
FTIR Spectroscopy to Reveal Lipid and Protein Changes Induced on Sperm by Capacitation: Bases for an Improvement of Sample Selection in ART
Authors: Pachetti Maria , Zupin Luisa , Venturin Irene , Mitri Elisa , Boscolo Rita , D’Amico Francesco , Vaccari Lisa , Crovella Sergio , Ricci Giuseppe , Pascolo Lorella
Published: November 2020

Although being a crucial step for Assisted Reproduction Technologies (ART) success, to date sperm selection is based only on morphology, motility and concentration characteristics. Considering the many possible alterations, there is a great need for analytical approaches allowing more effective sperm selections. The use of Fourier Transform Infrared (FTIR) may represent an interesting possibility, being able to reveal many macromolecular changes in a single measurement in a nondestructive way. As a proof of concept, in this observational study, we used a FTIR approach to reveal features related to sperm quality and chemical changes promoted by in vitro capacitation. We found indication that α-helix content is increased in capacitated sperm, while high percentages of the β-structures seem to correlate to poor-quality spermatozoa. The most interesting observation was related to the lipid composition, when measured as CH2/CH3 vibrations (ratio 2853/2870), which resulted in being strongly influenced by capacitation and well correlated with sperm motility. Interestingly, this ratio is higher than 1 in infertile samples, suggesting that motility is related to sperm membranes stiffness and lipid composition. Although further analyses are requested, our results support the concept that FTIR can be proposed as a new smart diagnostic tool for semen quality assessment in ART.
Upregulation of iNOS Protects Cyclic Mechanical Stretch-Induced Cell Death in Rat Aorta Smooth Muscle Cells
Authors: Zhao Jing , Nakahira Kiichi , Kimura Akihiko , Kyotani Yoji , Yoshizumi Masanori
Published: November 2020

Aortic dissection and aneurysm are associated with abnormal hemodynamic loads originating from hypertension. Our previous study demonstrated that cyclic mechanical stretch (CMS, mimicked hypertension) caused the death of rat aortic smooth muscle cells (RASMCs) in a mitogen activated-protein kinases (MAPKs)-dependent manner. The current study investigated the effects of inducible nitric oxide synthase (iNOS) on CMS-induced RASMC death. cDNA microarrays for CMS-treated RASMCs showed that iNOS expression levels were increased in response to CMS. Real-time polymerase chain reaction (PCR) analysis demonstrated that this increase was p38 MAPK (p38)-dependent. NO production was also increased. This increase could be inhibited by p38 and iNOS inhibitors. Thus, CMS-induced iNOS synthesized NO. CMS-induced cell death in RASMCs was increased by the iNOS inhibitor but abrogated by the long-acting NO donor DETA-NONOate. Increased iNOS expression was confirmed in the abdominal aortic constriction mouse model. Signal transducers and activators of transcription 1 (STAT1) was activated in stretched RASMCs, and iNOS expression and NO production were inhibited by the STAT1 inhibitor nifuroxazide. Our findings suggest that RASMCs were protected by iNOS from CMS-stimulated cell death through the STAT1 and p38 signal pathways independently.
Pseudomonas aeruginosa Biofilms
Authors: Thi Minh Tam Tran , Wibowo David , Rehm Bernd H.A.
Published: November 2020

Pseudomonas aeruginosa is an opportunistic human pathogen causing devastating acute and chronic infections in individuals with compromised immune systems. Its highly notorious persistence in clinical settings is attributed to its ability to form antibiotic-resistant biofilms. Biofilm is an architecture built mostly by autogenic extracellular polymeric substances which function as a scaffold to encase the bacteria together on surfaces, and to protect them from environmental stresses, impedes phagocytosis and thereby conferring the capacity for colonization and long-term persistence. Here we review the current knowledge on P. aeruginosa biofilms, its development stages, and molecular mechanisms of invasion and persistence conferred by biofilms. Explosive cell lysis within bacterial biofilm to produce essential communal materials, and interspecies biofilms of P. aeruginosa and commensal Streptococcus which impedes P. aeruginosa virulence and possibly improves disease conditions will also be discussed. Recent research on diagnostics of P. aeruginosa infections will be investigated. Finally, therapeutic strategies for the treatment of P. aeruginosa biofilms along with their advantages and limitations will be compiled.
Transthyretin Stabilization: An Emerging Strategy for the Treatment of Alzheimer’s Disease?
Authors: Saponaro Federica , Kim Jin Hae , Chiellini Grazia
Published: November 2020

Transthyretin (TTR), previously named prealbumin is a plasma protein secreted mainly by the liver and choroid plexus (CP) that is a carrier for thyroid hormones (THs) and retinol (vitamin A). The structure of TTR, with four monomers rich in β-chains in a globular tetrameric protein, accounts for the predisposition of the protein to aggregate in fibrils, leading to a rare and severe disease, namely transthyretin amyloidosis (ATTR). Much effort has been made and still is required to find new therapeutic compounds that can stabilize TTR (“kinetic stabilization”) and prevent the amyloid genetic process. Moreover, TTR is an interesting therapeutic target for neurodegenerative diseases due to its recognized neuroprotective properties in the cognitive impairment context and interestingly in Alzheimer’s disease (AD). Much evidence has been collected regarding the neuroprotective effects in AD, including through in vitro and in vivo studies as well as a wide range of clinical series. Despite this supported hypothesis of neuroprotection for TTR, the mechanisms are still not completely clear. The aim of this review is to highlight the most relevant findings on the neuroprotective role of TTR, and to summarize the recent progress on the development of TTR tetramer stabilizers.
miR-27b Modulates Insulin Signaling in Hepatocytes by Regulating Insulin Receptor Expression
Authors: Benito-Vicente Asier , Uribe Kepa B. , Rotllan Noemi , Ramírez Cristina M. , Jebari-Benslaiman Shifa , Goedeke Leigh , Canfrán-Duque Alberto , Galicia-García Unai , Saenz De Urturi Diego , Aspichueta Patricia , Suárez Yajaira , Fernández-Hernando Carlos , Martín Cesar
Published: November 2020

Insulin resistance (IR) is one of the key contributing factors in the development of type 2 diabetes mellitus (T2DM). However, the molecular mechanisms leading to IR are still unclear. The implication of microRNAs (miRNAs) in the pathophysiology of multiple cardiometabolic pathologies, including obesity, atherosclerotic heart failure and IR, has emerged as a major focus of interest in recent years. Indeed, upregulation of several miRNAs has been associated with obesity and IR. Among them, miR-27b is overexpressed in the liver in patients with obesity, but its role in IR has not yet been thoroughly explored. In this study, we investigated the role of miR-27b in regulating insulin signaling in hepatocytes, both in vitro and in vivo. Therefore, assessment of the impact of miR-27b on insulin resistance through the hepatic tissue is of special importance due to the high expression of miR-27b in the liver together with its known role in regulating lipid metabolism. Notably, we found that miR-27b controls post-transcriptional expression of numerous components of the insulin signaling pathway including the insulin receptor (INSR) and insulin receptor substrate 1 (IRS1) in human hepatoma cells. These results were further confirmed in vivo showing that overexpression and inhibition of hepatic miR-27 enhances and suppresses hepatic INSR expression and insulin sensitivity, respectively. This study identified a novel role for miR-27 in regulating insulin signaling, and this finding suggests that elevated miR-27 levels may contribute to early development of hepatic insulin resistance.
The Molecular and Functional Characteristics of HLA-G and the Interaction with Its Receptors: Where to Intervene for Cancer Immunotherapy?
Authors: Attia Jiji V. D. , Dessens Charlotte E. , van de Water Ricky , Houvast Ruben D. , Kuppen Peter J. K. , Krijgsman Daniëlle
Published: November 2020

Human leukocyte antigen G (HLA-G) mediates maternal-fetal immune tolerance. It is also considered an immune checkpoint in cancer since it may mediate immune evasion and thus promote tumor growth. HLA-G is, therefore, a potential target for immunotherapy. However, existing monoclonal antibodies directed against HLA-G lack sufficient specificity and are not suitable for immune checkpoint inhibition in a clinical setting. For this reason, it is essential that alternative approaches are explored to block the interaction between HLA-G and its receptors. In this review, we discuss the structure and peptide presentation of HLA-G, and its interaction with the receptors Ig-like transcript (ILT) 2, ILT4, and Killer cell immunoglobulin-like receptor 2DL4 (KIR2DL4). Based on our findings, we propose three alternative strategies to block the interaction between HLA-G and its receptors in cancer immunotherapy: (1) prevention of HLA-G dimerization, (2) targeting the peptide-binding groove of HLA-G, and (3) targeting the HLA-G receptors. These strategies should be an important focus of future studies that aim to develop immune checkpoint inhibitors to block the interaction between HLA-G and its receptors for the treatment of cancer.
Increased C-X-C Motif Chemokine Ligand 12 Levels in Cerebrospinal Fluid as a Candidate Biomarker in Sporadic Amyotrophic Lateral Sclerosis
Authors: Andrés-Benito Pol , Povedano Mònica , Domínguez Raúl , Marco Carla , Colomina Maria J. , López-Pérez Óscar , Santana Isabel , Baldeiras Inês , Martínez-Yelámos Sergio , Zerr Inga , Llorens Franc , Fernández-Irigoyen Joaquín , Santamaría Enrique , Ferrer Isidro
Published: November 2020

Sporadic amyotrophic lateral sclerosis (sALS) is a fatal progressive neurodegenerative disease affecting upper and lower motor neurons. Biomarkers are useful to facilitate the diagnosis and/or prognosis of patients and to reveal possible mechanistic clues about the disease. This study aimed to identify and validate selected putative biomarkers in the cerebrospinal fluid (CSF) of sALS patients at early disease stages compared with age-matched controls and with other neurodegenerative diseases including Alzheimer disease (AD), spinal muscular atrophy type III (SMA), frontotemporal dementia behavioral variant (FTD), and multiple sclerosis (MS). SWATH acquisition on liquid chromatography-tandem mass spectrometry (LC–MS/MS) for protein quantitation, and ELISA for validation, were used in CSF samples of sALS cases at early stages of the disease. Analysis of mRNA and protein expression was carried out in the anterior horn of the lumbar spinal cord in post-mortem tissue of sALS cases (terminal stage) and controls using RTq-PCR, and Western blotting, and immunohistochemistry, respectively. SWATH acquisition on liquid chromatography-tandem mass spectrometry (LC–MS/MS) revealed 51 differentially expressed proteins in the CSF in sALS. Receiver operating characteristic (ROC) curves showed CXCL12 to be the most valuable candidate biomarker. We validated the values of CXCL12 in CSF with ELISA in two different cohorts. Besides sALS, increased CXCL12 levels were found in MS but were not altered in AD, SMA, and FTD. Therefore, increased CXCL12 levels in the CSF can be useful in the diagnoses of MS and sALS in the context of the clinical settings. CXCL12 immunoreactivity was localized in motor neurons in control and sALS, and in a few glial cells in sALS at the terminal stage; CXCR4 was in a subset of oligodendroglial-like cells and axonal ballooning of motor neurons in sALS; and CXCR7 in motor neurons in control and sALS, and reactive astrocytes in the pyramidal tracts in terminal sALS. CXCL12/CXCR4/CXCR7 axis in the spinal cord probably plays a complex role in inflammation, oligodendroglial and astrocyte signaling, and neuronal and axonal preservation in sALS.
Glycation Increases the Risk of Microbial Traversal through an Endothelial Model of the Human Blood-Brain Barrier after Use of Anesthetics
Authors: Weber Veronika , Olzscha Heidi , Längrich Timo , Hartmann Carla , Jung Matthias , Hofmann Britt , Horstkorte Rüdiger , Bork Kaya
Published: November 2020

The function of the human blood–brain barrier (BBB), consisting mainly of the basement membrane and microvascular endothelial cells, is to protect the brain and regulate its metabolism. Dysfunction of the BBB can lead to increased permeability, which can be linked with several pathologies, including meningitis, sepsis, and postoperative delirium. Advanced glycation end products (AGE) are non-enzymatic, posttranslational modifications of proteins, which can affect their function. Increased AGE levels are strongly associated with ageing and degenerative diseases including diabetes. Several studies demonstrated that the formation of AGE interfere with the function of the BBB and may change its permeability for soluble compounds. However, it is still unclear whether AGE can facilitate microbial traversal through the BBB and how small compounds including anesthetics modulate this process. Therefore, we developed a cellular model, which allows for the convenient testing of different factors and compounds with a direct correlation to bacterial traversal through the BBB. Our results demonstrate that both glycation and anesthetics interfere with the function of the BBB and promote microbial traversal. Importantly, we also show that the essential nutrient and antioxidant ascorbic acid, commonly known as vitamin C, can reduce the microbial traversal through the BBB and partly reverse the effects of AGE.
A Potential Theragnostic Regulatory Axis for Arthrofibrosis Involving Adiponectin (ADIPOQ) Receptor 1 and 2 (ADIPOR1 and ADIPOR2), TGFβ1, and Smooth Muscle α-Actin (ACTA2)
Authors: Bayram Banu , Owen Aaron R. , Dudakovic Amel , Dagneaux Louis , Turner Travis W. , Bettencourt Jacob W. , Limberg Afton K. , Tibbo Meagan E. , Morrey Mark E. , Sanchez-Sotelo Joaquin , Berry Daniel J. , Kocher Jean-Pierre A. , Wijnen Andre J. van , Abdel Matthew P.
Published: November 2020

(1) Background: Arthrofibrosis is a common cause of patient debility and dissatisfaction after total knee arthroplasty (TKA). The diversity of molecular pathways involved in arthrofibrosis disease progression suggest that effective treatments for arthrofibrosis may require a multimodal approach to counter the complex cellular mechanisms that direct disease pathogenesis. In this study, we leveraged RNA-seq data to define genes that are suppressed in arthrofibrosis patients and identified adiponectin (ADIPOQ) as a potential candidate. We hypothesized that signaling pathways activated by ADIPOQ and the cognate receptors ADIPOR1 and ADIPOR2 may prevent fibrosis-related events that contribute to arthrofibrosis. (2) Methods: Therefore, ADIPOR1 and ADIPOR2 were analyzed in a TGFβ1 inducible cell model for human myofibroblastogenesis by both loss- and gain-of-function experiments. (3) Results: Treatment with AdipoRon, which is a small molecule agonist of ADIPOR1 and ADIPOR2, decreased expression of collagens (COL1A1, COL3A1, and COL6A1) and the myofibroblast marker smooth muscle α-actin (ACTA2) at both mRNA and protein levels in basal and TGFβ1-induced cells. (4) Conclusions: Thus, ADIPOR1 and ADIPOR2 represent potential drug targets that may attenuate the pathogenesis of arthrofibrosis by suppressing TGFβ-dependent induction of myofibroblasts. These findings also suggest that AdipoRon therapy may reduce the development of arthrofibrosis by mediating anti-fibrotic effects in joint capsular tissues.
Why Methodology Is Important: Coffee as a Candidate Treatment for COVID-19?
Authors: Belaroussi Yaniss , Roblot Paul , Peiffer-Smadja Nathan , Delaye Thomas , Mathoulin-Pelissier Simone , Lemeux Joffrey , Le Moal Gwenaël , Caumes Eric , Roblot France , Bleibtreu Alexandre
Published: November 2020

Background: During this pandemic situation, some studies have led to hasty conclusions about Corona Virus Disease-19 (COVID-19) treatment, due to a lack of methodology. This pedagogic study aimed to highlight potential biases in research on COVID-19 treatment. Methods: We evaluate the effect of coffee’s active part, 1,3,7-trimethylxanthine (TMX) on patients with COVID-19. A cohort of 93 patients, with a diagnosis of COVID-19 is analyzed. Results: TMX group and control group included, respectively, 26 and 67 patients. In the TMX group, patients had a median length of stay in hospital of 5.5 days shorter than in the control group (9.5 vs. 15 days, p < 0.05). Patients in the control group were more severe than patients in the TMX group with a significantly higher National Early Warning Score 2 (NEWS-2 score) (8 vs. 6, p = 0.002). Conclusions: Multiple biases prevents us from concluding to an effect of coffee on COVID-19. Despite an important social pressure during this crisis, methodology and conscientiousness are the best way to avoid hasty conclusions that can be deleterious for patients. Identifier: NCT04395742.
Digital Twin Based Optimization of a Manufacturing Execution System to Handle High Degrees of Customer Specifications
Authors: Barni Andrea , Pietraroia Dario , Züst Simon , West Shaun , Stoll Oliver
Published: November 2020

Lean production principles have greatly contributed to the efficient and customer-oriented mass production of goods and services. A core element of lean production is the focus on cycle times and designing production controls and buffers around any bottlenecks in the system. Hence, a production line organized by lean principles will operate in a static or at least quasi-static way. While the individualization of products is an interesting business approach, it can influence cycle times and in-time production. This work demonstrates how performance losses induced by highly variable cycle times can be recovered using a digital twin. The unit under analysis is an industrial joiner’s workshop. Due to the high variance in cycle time, the joinery fails its production target, even if all machines are below 80% usage. Using a discrete event simulation of the production line, different production strategies can be evaluated efficiently and systematically. It is successfully shown that the performance losses due to the highly variable cycle times can be compensated using a digital twin in combination with optimization strategies. This is achieved by operating the system in a non-static mode, exploiting the flexibilities within the systems.
Underwater Glider Propulsion Systems VBS Part 1: VBS Sizing and Glider Performance Analysis
Authors: Orozco-Muñiz Juan Pablo , Salgado-Jimenez Tomas , Rodriguez-Olivares Noe Amir
Published: November 2020

The Variable Buoyancy System (VBS) is a critical device in the operation of underwater gliders that should be properly sized to achieve the required vehicle propulsion; safety within the operating range; and adequate efficiency at the nominal depth rating. The VBS budget volume depends mainly on the glider hydrodynamics and the main operating states of the vehicle. A method is proposed with analytical equations to analyze the performance of underwater gliders and to estimate the resultant velocities of the vehicle as a function of the buoyancy change and the glider angle. The method is validated to analyze the glider performance of underwater gliders and is essential to get the main design requirement for the propulsion system: the VBS budget volume. The paper presents the application of the method to obtain the VBS sizing for an academic glider; a comparison with the historical hydrodynamic data of the Slocum glider; the results of the glider performance study; and the development of the characteristic charts necessary to evaluate the performance of the vehicle and its flight parameters.
Observations and Preliminary Vulnerability Assessment of a Hybrid Dune-Based Living Shoreline
Authors: Winters Maria A. , Leslie Brian , Sloane Evyan Borgnis , Gallien Timu W.
Published: November 2020

A novel hybrid (e.g., vegetation, sand, cobble, rip-rap) nature-based dune structure was constructed at Cardiff State Beach in Encinitas, California, to protect a critical transportation artery from undermining and frequent flooding. A collaboration between regulators, funders, state agencies, professional practice and academia developed a high resolution robust unmanned aerial vehicle (UAV) based monitoring strategy to observe dune construction and evolution. Fifteen construction surveys were conducted to observe each substrate element for future morphodynamic modeling efforts. Six post-construction surveys were conducted to observe seasonal and storm-by-storm dune evolution. Backshore vulnerability was assessed using a sixty-one year time series of tides and hindcast wave forcing fit to a general extreme value distribution. The dune crest is above calculated 100-year water levels; however, the dune remains vulnerable to mass wasting caused by swash interaction at the toe of the dune. Sea-level rise will substantially increase the probability of dune erosion, breaching, and overtopping.
Starvation-Dependent Inhibition of the Hydrocarbon Degrader Marinobacter sp. TT1 by a Chemical Dispersant
Authors: Rughöft Saskia , Vogel Anjela L. , Joye Samantha B. , Gutierrez Tony , Kleindienst Sara
Published: November 2020

During marine oil spills, chemical dispersants are used routinely to disperse surface slicks, transferring the hydrocarbon constituents of oil into the aqueous phase. Nonetheless, a comprehensive understanding of how dispersants affect natural populations of hydrocarbon-degrading bacteria, particularly under environmentally relevant conditions, is lacking. We investigated the impacts of the dispersant Corexit EC9500A on the marine hydrocarbon degrader Marinobacter sp. TT1 when pre-adapted to either low n-hexadecane concentrations (starved culture) or high n-hexadecane concentrations (well-fed culture). The growth of previously starved cells was inhibited when exposed to the dispersant, as evidenced by 55% lower cell numbers and 30% lower n-hexadecane biodegradation efficiency compared to cells grown on n-hexadecane alone. Cultures that were well-fed did not exhibit dispersant-induced inhibition of growth or n-hexadecane degradation. In addition, fluorescence microscopy revealed amorphous cell aggregate structures when the starved culture was exposed to dispersants, suggesting that Corexit affected the biofilm formation behavior of starved cells. Our findings indicate that (previous) substrate limitation, resembling oligotrophic open ocean conditions, can impact the response and hydrocarbon-degrading activities of oil-degrading organisms when exposed to Corexit, and highlight the need for further work to better understand the implications of environmental stressors on oil biodegradation and microbial community dynamics.
Thermal Stress Analysis of Process Piping System Installed on LNG Vessel Subject to Hull Design Loads
Authors: Hwang Se-Yun , Kim Min-Seok , Lee Jang-Hyun
Published: November 2020

In this paper, the procedure for the strength evaluation of the piping system installed on liquefied natural gas (LNG) carriers is discussed. A procedure that accounts for the ship’s wave load and hull motion acceleration (as well as the deformation due to the thermal expansion and contraction experienced by the hull during seafaring operations) is presented. The load due to the temperature and self-weight of the piping installed on the deck is also considered. Various operating and load conditions of the LNG piping system are analyzed. Stress analysis is performed by combining various conditions of sustained, occasional, and expansion loads. Stress is assessed using finite element analysis based on beam elements that represent the behavior of the piping. The attributes of the piping system components (such as valves, expansion joints, and supports) are represented in the finite element model while CAESAR-II, a commercial software is used for finite element analysis. Component modeling, load assignment, and load combinations are presented to evaluate pipe stresses under various load conditions. An evaluation model is selected for the piping arrangement of LNG and the evaluated stress is compared with the allowable stress defined by the American Society of Mechanical Engineers (ASME).
A Fast Algorithm for the Prediction of Ship-Bank Interaction in Shallow Water
Authors: Huang Jin , Xu Chen , Xin Ping , Zhou Xueqian , Sutulo Serge , Guedes Soares Carlos
Published: November 2020

The hydrodynamic interaction induced by the complex flow around a ship maneuvering in restricted waters has a significant influence on navigation safety. In particular, when a ship moves in the vicinity of a bank, the hydrodynamic interaction forces caused by the bank effect can significantly affect the ship’s maneuverability. An efficient algorithm integrated in onboard systems or simulators for capturing the bank effect with fair accuracy would benefit navigation safety. In this study, an algorithm based on the potential-flow theory is presented for efficient calculation of ship-bank hydrodynamic interaction forces. Under the low Froude number assumption, the free surface boundary condition is approximated using the double-body model. A layer of sources is dynamically distributed on part of the seabed and bank in the vicinity of the ship to model the boundary conditions. The sinkage and trim are iteratively solved via hydrostatic balance, and the importance of including sinkage and trim is investigated. To validate the numerical method, a series of simulations with various configurations are carried out, and the results are compared with experiment and numerical results obtained with RANSE-based and Rankine source methods. The comparison and analysis show the accuracy of the method proposed in this paper satisfactory except for extreme shallow water cases.
Investigations of the Potential Application of k-out-of-n Systems in Oil and Gas Industry Objects
Authors: Rykov Vladimir V. , Sukharev Mikhail G. , Itkin Victor Yu.
Published: November 2020

The purpose of this paper was to demonstrate the possibilities of assessing the reliability of oil and gas industry structures with the help of mathematical models of k-out-of-n systems. We show how the reliability of various structures in the oil and gas complex can be described and investigated using k-out-of-n models. Because the initial information about the life and repair time of components of systems is only usually known on the scale of one and/or two moments, we focus on the problem of the sensitivity analysis of the system reliability indices to the shape of its components repair time distributions. To address this problem, we used the so-called markovization method, based on the introduction of supplementary variables, to model the system behavior with the help of the two-dimensional Markov process with discrete-continuous states. On the basis of the forward Kolmogorov equations for the time-dependent process’ state probabilities, relevant balance equations for the process’ stationary probabilities are presented. Using these equations, stationary probabilities and some reliability indices for two examples from the oil and gas industry were calculated and their sensitivity to the system component’s repair time distributions was analyzed. Calculations show that under “rare” component failures, most system reliability indices become practically insensitive to the shape of the components repair time distributions.
A Simplified Numerical Method for the Design and Analysis of FPSO Platform Brackets Subjected to Operational Conditions
Authors: Martins Kauê L. , Pinto Vinícius T. , Fragassa Cristiano , Real Mauro V. , Rocha Luiz A. O. , Isoldi Liércio A. , dos Santos Elizaldo D.
Published: November 2020

The present work develops a simplified computational methodology to predict brackets’ mechanical behavior to support mooring systems’ fairleads under maximum environmental loads. The methodology consists of estimating environmental loads in Campos’s basin (Brazil) using standards available in the literature and applying them to determine the stress distribution in the brackets’ solid domain using a code based on the Finite Element Method. The brackets studied here are composed of AH36 steel plates. Six different geometric configurations for the brackets considering thinner plates than those ordinarily employed in the existent brackets of the P-66 Petrobras oil production platform were studied. Theoretical recommendations about geometric configurations to reduce the von Mises maximum stress in brackets’ plates were determined in the light of constructal design. The results proved the importance of the geometric investigation in this problem. For instance, for the accidental limit state (ALS) the best configuration leads to a mechanical performance two times superior to that reached for the worst one, which had a maximum stress superior to the yielding limit. Moreover, the best designs for the superior and inferior brackets in the ALS have different configurations.
Dynamics of the Land–Sea Breeze System and the Surface Current Response in South-West Australia
Authors: Rafiq Syeda , Pattiaratchi Charitha , Janeković Ivica
Published: November 2020

The land–sea breeze (LSB) system, driven by the thermal contrast between the land and the adjacent ocean is a widely known atmospheric phenomenon, which occurs in coastal regions globally. South-west Australia experiences a persistent and one of the strongest LSB systems globally with maximum wind speeds associated with the LSB system often exceeding 15 ms−1. In this paper, using field measurements and numerical simulations, we examine: (1) the local winds associated with the land–sea breeze with an emphasis on the ocean; and, (2) the response of the surface currents to the diurnal wind forcing. The measurements indicated that the wind speeds decreased between midnight and 0400 and increased rapidly after 1100, reaching maxima >10 ms−1 around 1800) associated with the sea breeze and decreased to midnight. Wind directions were such that they were blowing from south-east (120°) in the morning and changed to almost southerly (~200°) in the afternoon. Decomposition of the wind record to the diurnal and synoptic components indicated that the diurnal component of winds (i.e., LSB) was oriented along the south-west to north-east axis. However, the stronger synoptic winds were from the south-east to south quadrant and in combination with the LSB, the winds consisted of a strong southerly component. We examined the evolution, horizontal extent, and propagation properties of sea breeze fronts for characteristic LSB cycles and the sea breeze cell propagating offshore and inland. The results indicated that the sea breeze cell was initiated in the morning in a small area, close to 33° S, 115.5° E, with a width of ~25 km and expanded onshore, offshore and alongshore. The sea breeze cell expanded faster (30 kmh−1) and farther (120 km) in the offshore direction than in the onshore direction (10 kmh−1 and 30–40 km). Winds during the LSB cycle followed a counterclockwise rotation that was also reflected in the surface currents. The winds and surface currents rotated anticlockwise with the surface currents responding almost instantaneously to changes in wind forcing but were modified by topography. The diurnal surface currents were enhanced due to the resonance between the LSB forcing and the inertial response.
The Wave Energy Converter Design Process: Methods Applied in Industry and Shortcomings of Current Practices
Authors: Trueworthy Ali , DuPont Bryony
Published: November 2020

Wave energy is among the many renewable energy technologies being researched and developed to address the increasing demand for low-emissions energy. The unique design challenges for wave energy converter design—integrating complex and uncertain technological, economic, and ecological systems, overcoming the structural challenges of ocean deployment, and dealing with complex system dynamics—have lead to a disjointed progression of research and development. There is no common design practice across the wave energy industry and there is no published synthesis of the practices that are used by developers. In this paper, we summarize the methods being employed in WEC design as well as promising methods that have yet to be applied. We contextualize these methods within an overarching design process. We present results from a survey of WEC developers to identify methods that are common in industry. From the review and survey results, we conclude that the most common methods of WEC design are iterative methods in which design parameters are defined, evaluated, and then changed based on evaluation results. This leaves a significant space for improvement of methods that help designers make better-informed decisions prior to sophisticated evaluation, and methods of using the evaluation results to make better design decisions during iteration. Despite the popularity of optimization methods in academic research, they are less common in industry development. We end this paper with a summary of the areas of WEC design in which the testing and development of new methods is necessary, and where more research is required to fully understand the influence of design decisions on WEC performance.
21 May 2003 Boumerdès Earthquake: Numerical Investigations of the Rupture Mechanism Effects on the Induced Tsunami and Its Impact in Harbors
Authors: Masina Marinella , Archetti Renata , Lamberti Alberto
Published: November 2020

In order to obtain a fair and reliable description of the wave amplitude and currents in harbors due to the tsunami generated by the 21 May 2003 Boumerdès earthquake (Algeria), a numerical investigation has been performed with a standard hydraulic numerical model combined with various source fault models. Seven different rupture models proposed in literature to represent high frequency seismic effects have been used to simulate tsunami generation. The tsunami wave propagation across the Western Mediterranean Sea and in bays and harbors of the Balearic Islands is simulated, and results are checked against sea level measurements. All of them resulted in a significant underestimation of the tsunami impact on the Balearic coasts. In the paper the best fitting source model is identified, justifying the energy intensification of the event to account for low frequency character of tsunami waves. A fair correspondence is pointed out between damages to boats and harbor infrastructures, reported in newspapers, and wave intensity, characterized by level extremes and current intensity. Current speed and amplitude thresholds for possible damage in harbors suggested respectively by Lynett et al., doi.org/10.1002/2013GL058680, and Muhari et al., doi.org/10.1007/s11069-015-1772-0, are confirmed by the present analysis.
Melanin as a Virulence Factor in Different Species of Genus Paracoccidioides
Authors: C. P. Emidio Elúzia , E. Urán J. Martha , B. R. Silva Leandro , S. Dias Lucas , Doprado Mariana , Nosanchuk Joshua D. , Taborda Carlos Pelleschi
Published: November 2020

Paracoccidioidomycosis (PCM) is a granulomatous systemic mycosis caused by the thermo-dimorphic fungi of the genus Paracoccidioides. Melanin production by fungi can affect their pathogenesis and virulence. This study evaluates the production of melanin by different isolates of genus Paracoccidioides and examines how the presence of this polymer affects yeast cell phagocytosis, as well as laccase enzyme production. The results obtained showed that the isolates of genus Paracoccidioides: P. lutzii (Pb01, Pb66, ED01, Pb1578, and Pb8334), P. restrepiensis (PS3-Pb60855), P. brasiliensis (S1-Pb18), and P. americana (PS2-Pbcão) produce melanin in the presence of L-3,4-dihydroxyphenylalanine (L-DOPA). Phagocytosis assays were carried out with peritoneal macrophages from C57Bl/6 mice that were challenged with Pb18, Pb60855, and Pb01. We observed that melanin interferes with phagocytosis in the presence or absence of complement or heat-inactivated serum. This article confirms that different species of the genus Paracoccidioides produce melanin in different magnitudes and that the polymer functions as a virulence factor.
The Textuality of the Modernist Rural Landscape: Belgrade Agricultural Combine (PKB) as a Driver of the Urban Development of Third Belgrade
Authors: Djokić Vladan , Milovanović Aleksandra , Ristić Trajković Jelena
Published: November 2020

This paper considers the landscape as both a material and an ideological representation and starts from the assumption that spatial patterns arise as a result of the ideological imperative of the process that forms the landscape. The research takes on a historical-interpretative approach in the domain of architectural and urban studies, enabling in-depth qualitative exploration of the textuality and layering of the modernist rural landscape through a case study of the PKB Agricultural Combine as a driver of the urban development of Third Belgrade, the spatial framework of the left riverbank of the Danube in the administrative area of Belgrade. The research was conducted by chronologically interpreting primary sources, notably planning documents of different levels and scope, as well as studies, programs and development models for the urbanization of this territory. The research aims to decode the impact of socialist agrarian policy on the land-use in the wider metropolitan area of Belgrade, as well as the impact of the agricultural combine as a spatial, social, economic, environmental and political entity on the urban development process at different spatial levels. The research has identified four periods in the development of Third Belgrade: (1) Production of the Modernist Rural Landscape, (2) Establishment of the Self-Management Planning Framework, (3) Humanization of Environment, and (4) Post-socialist Transition and the Collapse of the Agricultural Combine. The paper demonstrates not only that environmental transformation cannot be separated from social transformation but also that they are in constant interaction and that their synergy has had a profound impact on the development of the PKB Agricultural Combine system in socialist conditions. The textuality of the modernist rural landscape confirms that an object-oriented approach is not enough to explore and interpret the landscape, but rather, we should look at the way it is socially produced through decoding the planning, institutional and policy frameworks determining the urban development of a territory.
Comparison of the Physicochemical Properties of Carboxylic and Phosphonic Acid Self-Assembled Monolayers Created on a Ti-6Al-4V Substrate
Authors: Cichomski Michal , Prowizor Milena , Kowalczyk Dorota Anna , Sikora Andrzej , Batory Damian , Dudek Mariusz
Published: November 2020

This study compared the tribological properties in nano- and millinewton load ranges of Ti‑6Al-4V surfaces that were modified using self-assembled monolayers (SAMs) of carboxylic and phosphonic acids. The effectiveness of the creation of SAMs with the use of the liquid phase deposition (LPD) technique was monitored by the contact angle measurement, the surface free energy (SFE) calculation, X-ray photoelectron spectroscopy (XPS), and Fourier-transform infrared spectroscopy (FTIR) measurements. The obtained results indicated that more stable and well-ordered layers, which were characterized by the lowest values of the coefficient of friction, adhesion, and wear rate, were obtained using phosphonic acid as a surface modifier. Based on the obtained results, it was found that the Ti-6Al-4V alloy modified by phosphonic acid would be the most advantageous for practical applications, especially in micro- and nanoelectromechanical systems (MEMS/NEMS).