bluuebunny/crossref_metadata_2025 huggingface dataset:
Obsessive–compulsive existential type: a dialectical-phenomenological approach
Authors: Fukuda Lívia , Tamelini Melissa , Messas Guilherme
Published: September 2023

The clinical presentation of obsessive–compulsive patients is characterized by unwanted, intrusive, nonsensical, self-related, and recurrent ideas, thoughts, images, or impulses associated with active compulsive compensations. Under the operational diagnostic criteria adopted by the biological- and cognitive-oriented neopositivist medical paradigm, it is known as “obsessive–compulsive disorder.” However, this paradigm has been criticized for its controversial assumptions, limited methodologies, theoretic biases, and inconsistency in producing practical outcomes. To bypass some of these issues, we propose a complementary approach that draws on and further develops existing psychopathological studies of the obsessive–compulsive anthropological condition based on dialectical phenomenological psychopathology. As such, we refer to the global clinical configuration as the “obsessive–compulsive existential type.” Our theoretical inspiration comes from the classical phenomenological work on obsessions undertaken by Straus and Gebsattel, which identified the negative transformation of the obsessive–compulsive life-world or the endogenous emergence of the anti-eidos (diluting existential force). We then propose to broaden the concept of anti-eidos, especially in its dialectical correlation with eidos (unifying existential force), representing the existential dialectic between transformation and permanence. Next, we detail the dynamics of anthropological disproportions in obsessive–compulsive existential type, essentially the supremacy of the anti-eidos over the eidos. This primary imbalance modifies the obsessive–compulsive existential structure, consisting of polymorphic temporality; weakened intentionality; maladjusted calibration of distance with the world and others; an integral, isolated, besieged self with dwindling self-agency, and tense and over-protecting embodiment. We also analyze compensatory hyperreflexivity and compulsive rituals as expressions of structural counterbalancing designed to contain the primary structural disproportions and derangements. The heterogeneous obsessive–compulsive clinical manifestations are the complex result of the primary structural alteration and subsequent phenomenological compensations. They tend to be variable in temporal span and rarely assume a fixed form, hindering diagnosis. We correlate structural frameworks with multiple clinical examples. Finally, we raise some insights on how our study may contribute to scientific research and therapeutic proposals.
Fostering public climate change discussions from a social interaction perspective
Authors: Tian Jianchi , Zheng Xiaoqing , Sun Yan
Published: September 2023

Public discussions on climate change, as a form of social interaction, are widely recognized as effective tools for promoting collective action. However, there is limited research on examining the factors that influence climate change discussions from a social interaction perspective. In the present study, we conducted a large sample (N = 1,169) survey to investigate personal (such as self-efficacy and personal response efficacy) and others' (such as perceived others' response efficacy and social norms) factors influencing climate change discussions from a social interaction perspective. The results showed that (i) for people with high climate change perceptions, personal response efficacy, self-efficacy, and social norms have positive effects on climate change discussions, but the effect of perceived others' response efficacy on climate change discussion is not significant; (ii) for people with low climate change perceptions, self-efficacy and social norms have positive effects on climate change discussions, but the effects of personal response efficacy and perceived others' response efficacy on climate change discussion are not significant; (iii) irrespective of individuals' high or low perceptions of climate change, social norm remains the most important predictor of climate change discussions. These findings make valuable contributions to the theoretical literature and intervention efforts regarding climate change discussions from a social interaction perspective.
Quality of life in families under quarantine: a cross-sectional study in seven countries during the first outbreak of COVID-19
Authors: Moya-Salazar Jeel , Chiu-Higa Erika , Jaime-Quispe Alexis , Cañari Betsy , Moya-Espinoza Jeel G. , Contreras-Pulache Hans
Published: September 2023

Background

The COVID-19 pandemic has disrupted human well-being worldwide in unforeseen ways. In early 2020, the spread of the virus left its mark on every affected country, impacting mental health by limiting daily activities and causing fatalities amidst public health strategies to mitigate its impact. The influence of COVID-19 on the quality of life (QoL) may vary between countries, underscoring the need to examine its effects on individuals and families during the mandatory home quarantine. We aimed to assess the QoL of individuals and families during home isolation by COVID-19 lockdown.

Methods

A cross-sectional study was conducted from February to May 2020. We included adult partners (≥18 years) of families from Brazil, Colombia, Spain, Japan, Peru, Russia, and Venezuela. Using the 26-item World Health Organization Quality of Life Brief (WHOQOL-BREF) questionnaire we assess the impact of COVID-19 on their partner/family member’s QoL.

Results

The survey was completed by 466 participants (mean age = 38.59 ± 13.75 years; females = 298) and 76% worked mostly as health professionals from South America (69.2%), Europe (18.4%), and Asia (12.4%). The WHOQOL-BREF mean score from 38.38 ± 11.55 (range = 22.8–43.4). The average quality of life in South America (41.9 ± 1.2) was significantly higher than that of European countries (30.9 ± 11.5) (p = 0.002). The social relations dimension was the only one with values close to 100 (mean = 83.3) in 6/7 evaluated countries, where only Spain had a low score (41 ± 33.12). Women had a slightly lower quality of life than men, but it was not significant (40.2 ± 8.8 vs. 41.5 ± 9.9, p = 0.354), while we found differences in the overall QoL between young and older, and by employment type (p < 0.05). According to family structure, we found differences on QoL between nuclear and siblings’ families (p = 0.024).

Conclusion

Families from seven countries of three continents reported poor QoL during the first outbreak of COVID-19. The pandemic scenario has dramatically weakened the QoL in 3/4 dimensions, where social relationships have remained high. It is important to fully address the impact of this reduced QoL on families after several waves of infection and to provide comprehensive support in the post-COVID future.
Trends and profiles of acute poisoning cases: a retrospective analysis
Authors: Liu Shifang , Ling Lijing , Ma Jin , Yuan Hua , Guo Zhiqiang , Feng Qiupeng , Xia Xiaohua
Published: September 2023

Acute poisoning is a significant public health concern. This retrospective study investigates trends in acute poisoning cases and explores the clinical and sociodemographic profiles associated with this condition. Medical data from 859 hospitalized patients diagnosed with acute poisoning between January 2017 and December 2022 were comprehensively analyzed. The descriptive statistical analysis revealed that 360 patients had underlying diseases, with depression being the most prevalent among them. Furthermore, urban areas accounted for 87.2% of the acute poisoning cases, indicating a higher incidence compared to rural areas. The substances implicated in acute poisoning incidents varied, with drugs of abuse being the most common (53.2%), followed by pesticides (22.2%), carbon monoxide (11.8%), and alcohol (5.4%). Suicide attempt/suicide emerged as the leading cause of acute poisoning incidents, accounting for 75.9% of cases, while poisoning accidents predominantly occurred within the home setting. Through chi-square tests, it was determined that risk factors for suicide attempt/suicide included female gender and underlying medical conditions. Temporal analysis showed that the total number of acute poisoning cases increased from 2017 to 2019 and decreased from 2019 to 2022. Notably, suicide-related cases exhibited an upward trend, with suicide attempt/suicide accounting for over 80% of all acute poisoning cases after 2020. This study contributes valuable insights into the trends, profiles, and risk factors associated with acute poisoning cases.
Neurocognitive outcomes of children exposed to and living with HIV aged 3–5 years in Kilifi, Kenya
Authors: Chongwo Esther Jebor , Wedderburn Catherine J. , Nyongesa Moses Kachama , Sigilai Antipa , Mwangi Paul , Thoya Janet , Odhiambo Rachel , Ngombo Katana , Kabunda Beatrice , Newton Charles R. , Abubakar Amina
Published: September 2023

Introduction

Globally, 1.7 million children are living with HIV, with the majority of them residing in sub-Saharan Africa. Due to reduced rates of vertical transmission of HIV, there is an increasing population of children born to HIV-infected mothers who remain uninfected. There is a growing concern around the development of these children in the antiretroviral therapy era. This study examined the neurocognitive outcomes of children who are HIV-exposed infected (CHEI), HIV-exposed uninfected (CHEU) and HIV-unexposed uninfected (CHUU) and explored the relationship between child neurocognitive outcomes and child's biomedical and caregivers’ psychosocial factors.

Methods

CHEI, CHUU and CHEU aged 3–5 years and their caregivers were recruited into the study. Neurocognitive outcomes were assessed using a validated battery of assessments. One-way analysis of variance and covariance (ANOVA and ANCOVA) were used to evaluate differences among the three groups by neurocognitive outcomes. Linear regression models were used to investigate the association between child neurocognitive outcomes and biomedical factors (nutritional status, HIV disease staging) and caregivers’ psychosocial factors [symptoms of common mental disorders (CMDs) and parenting behaviour].

Results

The study included 153 children and their caregivers: 43 (28.1%) CHEI, 52 (34.0%) CHEU and 58 (39.9%) CHUU. ANOVA and ANCOVA revealed a significant difference in cognitive ability mean scores across the child groups. Post hoc analysis indicated that CHEU children had higher cognitive ability mean scores than the CHUU group. Better nutritional status was significantly associated with higher cognitive ability scores (β = 0.68, 95% CI [0.18–1.18], p = 0.008). Higher scores of CMDs were negatively associated with inhibitory control (β = −0.28, 95% CI [−0.53 to 0.02], p = 0.036). While comparing HIV stages 2 and 3, large effect sizes were seen in working memory (0.96, CI [0.08–1.80]) and cognitive ability scores (0.83 CI [0.01–1.63]), indicating those in stage 3 had poor performance.

Conclusions

Neurocognitive outcomes were similar across CHEI, CHEU and CHUU, although subtle differences were seen in cognitive ability scores where CHEU had significantly higher cognitive mean scores than the CHUU. Well-designed longitudinal studies are needed to ascertain these findings. Nonetheless, study findings underscore the need for strategies to promote better child nutrition, mental health, and early antiretroviral therapy initiation.
Is non-mentored initiation of laparoscopic colorectal surgery safe? Single surgeon initial experience with the first 40 cases
Authors: Bakula Branko
Published: September 2023

Introduction

Although laparoscopic colorectal surgery is now accepted as a standard procedure in treating colorectal cancer, the proportion of laparoscopically operated patients with colorectal cancer is still generally quite low. The aim of this study is to assess feasibility, safety, and outcomes of a non-mentored initiation of laparoscopic colorectal resections by a young surgeon without previous experience in laparoscopic colorectal surgery.

Materials and methods

We analyzed the characteristics of the first 40 elective cases of laparoscopic colorectal resections performed by a single surgeon during the period between June 2019 and March 2022. All of the operations were performed without the attendance or supervision of an experienced surgeon in laparoscopic colorectal surgery. The patients were divided into three groups (the early, intermediate, and late group).

Results

The conversion rate, complications rate, and postoperative recovery were similar among groups. The mean overall operative time was 219.5 min (range 130–420 min) and had reduced significantly during the learning curve (p = 0.047). The overall conversion rate was 12.5%. In two cases (5%), the oncological principles were violated (incomplete total mesorectal excision). In three patients (7.5%), intraoperative complications had occurred (small bowel injury, splenic injury, and significant bleeding from the minor peripancreatic artery). Three cases of major postoperative complications (Clavien–Dindo grade III) were recorded, two of which required reoperation (anastomotic bleeding and fascial dehiscence). There was no 90-day mortality reported. The overall mean number of lymph nodes retrieved was 12.45, which did not differ significantly among groups (p = 0.678). The average follow-up was 13.75 months (range 1–31 months). Cancer recurrence was recorded in four patients (10%). Port-site metastasis was not detected in any of the cases.

Conclusion

A safe and non-mentored initiation of laparoscopic colorectal surgery with an acceptable rate of complications and acceptable oncological results can be achieved. Still, when compared with a structured initiation in a controlled environment with the supervision of an experienced surgeon in laparoscopic colorectal surgery, the results of a non-mentored initiation are worse in most of the fields, including operative time, conversion rate, complications rate, and duration of hospital stay. Therefore, I strongly recommend engaging young surgeons in fellowship programs on structured laparoscopic colorectal surgery whenever possible before starting performing these procedures on their own.
Examining the Theory of Planned Behaviour in Green Purchasing Behaviour – A Case of Millennial’s Consumption of Organic Food
Authors: Ali Bakhtiar , Fatima Mehreen , Ahmed Qazi Mohammed
Published: September 2023

The study aimed to better understand how millennials' attitudes, buying intentions, and behavior related to green products with the help of the theory of planned behavior. The research also looked at the function of green purchase intentions (GPI), a subjective norm (SN), in mediating the affiliation between attitudes toward green products (AGP), perceived control behavior, and green purchasing behavior (GPB). The data was gathered from 256 millennials from Pakistan's twin cities using a well-structured questionnaire. The Smart PLS computer application was used to examine the data by means of the partial least squares (PLS) technique. Additionally, the results revealed that GPI had a notable impact on GPB. The interaction between AGP and GPB was mediated by GPI. Similarly, SN and GPB were also mediated by GPI. Promoting knowledge of the many factors influencing green purchase behavior, the study will assist policymakers and green marketers in formulating and putting into practice initiatives to stimulate green shopping.
Assessing Capacity in Rural Nonprofits
Authors: Walters Jayme , Brown Aaron R. , Wallis Dorothy , Snow Janice
Published: September 2023

Organizational capacity is necessary for nonprofits to meet their missions and contribute to society. Less is known about rural nonprofits’ organizational capacity and characteristics, as most research focuses on urban nonprofits. The present study first considers the utility of an organizational capacity assessment and identifies pertinent areas of organizational capacity in rural nonprofits. The second objective of the study is to examine relationships between areas of organizational capacity and organizational-level characteristics. In May 2019, nonprofits (n = 290) from persistently poor, rural counties in 14 southern states participated in a survey providing organizational characteristics and assessing organizational capacity. An exploratory factor analysis on the organizational capacity assessment revealed four organizational capacity areas: Organizational Identity, Fund Development, Volunteers, and Organizational Procedures. Then, descriptive, bivariate, and multivariate analyses were conducted to understand the relationships between the organizational capacity areas and organization characteristics, including organizational age, expenses, life stage, NTEE classification, and executive director tenure. Findings indicate significant relationships exist between organizational capacity and characteristics, consistent with previous studies. Older, mature, and/or larger rural nonprofits have increased capacity in several areas. Thus, targeting younger and smaller rural organizations for capacity-building efforts may be impactful.
Research on Differential Steering Dynamics Control of Four-Wheel Independent Drive Electric Tractor
Authors: An Yuhui , Wang Lin , Deng Xiaoting , Chen Hao , Lu Zhixiong , Wang Tao
Published: September 2023

Traditional tractors can only achieve steering through mechanical structures such as steering knuckles and steering trapezoids. Among them, the mechanical structure is more complex, and various parts are easily damaged, making the tractor malfunction. The four-wheel independent drive differential steering mode differs from the traditional Ackermann steering mode, which realizes steering by controlling the inner and outer wheel torque, which can accurately steer the working state of high-end agricultural machinery equipment and improve the operating efficiency of agricultural machinery equipment. Aiming at the dynamic control problem in the steering of electric tractor four-wheel independent drive, a layered control strategy based on the sliding mode control of yaw torque at the upper level and the optimal torque distribution level based on the mean load rate of vehicle tires at the lower was proposed. By analyzing the differential steering mechanism of a four-wheel independent drive, a dynamic model of differential steering of the electric tractor is established, and a dynamic controller of a four-wheel independent drive is designed according to the layered control strategy. The upper controller tracks and controls the expected yaw speed on the basis of the sliding mode control to track the driver’s intention, and the lower controller realizes the optimal torque distribution based on the principle of the optimal average load rate of the vehicle tire to ensure the steering stability of the electric tractor. The effect of the controller was simulated and analyzed under typical conditions of double line shift, serpentine, and step. The results showed that the sliding mode controller is better than the PID controller in driver intention tracking. Compared with the average allocation strategy, the average maximum load rate of the vehicle tire under the three working conditions is reduced by 16.9%, 13.8%, and 17.3%, respectively, which proves the effectiveness of the layered control strategy. In the real car test, the sliding mode controller is better than the PID controller in the driver intention tracking. This study has important guiding significance for improving the maneuverability and stability of electric tractors.
The Impact of Multi-Dimensional Vectors on China’s Agricultural Products Export: Based on fsQCA
Authors: Yin Xiaomiao , Xing Lirong , Cui Chunxiao
Published: September 2023

Since 2004, China has been experiencing persistent and significant agricultural trade deficits. The longstanding unfavorable balance has induced a series of problems that require urgent attention. The fundamental approach to narrow the agricultural trade gap is to increase agricultural exports. Based on the cultural, institutional, geographical, and economic theoretical framework and using the fuzzy-set qualitative comparative analysis configurational approach, this study clarifies the configurational paths of the impact of multi-dimensional vectors on China’s agricultural exports in recent years. The findings show that (1) the multi-dimensional vectors configuration paths from 2016 to 2020 have certain similarities, and the impact paths are relatively stable; (2) the configuration paths of multi-dimensional vectors are different when comparison involves’ China’s high-level and non-high-level agricultural exports; (3) all the four vectors simultaneously affect high-level agricultural exports; and the absence of cultural and institutional vectors influence China’s non-high-level agricultural exports. Therefore, policymakers should note that one size does not fit all in promoting agricultural exports and that improving the overseas dissemination of a country’s culture and its institutional policies can greatly increase agricultural exports.
Effect of Pesticides and a Long-Life Inoculant on Nodulation Process and Soybean Seed Quality during Storage
Authors: Araújo Rodrigo S. , Silva Gisele C. , Teixeira Itamar R. , Silva Guilherme R. , Souza Bruna Mayara F. , Devilla Ivano A. , Araújo Marcos Eduardo V. , Corrêa Paulo C.
Published: September 2023

Inoculants containing strains of bacteria that fix atmospheric N that are tolerant or resistant to chemical products commonly used in seed treatment are classified as long-life inoculants, which contribute to improving the efficiency of nodulating bacteria in soybean crops. The objective of this work was to evaluate the effects of applying commonly used pesticides in seed treatment and a long-life inoculant, alone or in combination, on the nodulation process and seed quality of soybeans during storage. The first experiment was carried out in a greenhouse using a completely randomized design, in an 8 × 2 factorial arrangement, with three replications. The treatments consisted of applications of industrial seed treatments: control, MaximAdvanced, Fortenza, long-life inoculant, MaximAdvanced + Fortenza, MaximAdvanced + long-life inoculant, Fortenza + long-life inoculant, and MaximAdvanced + Fortenza + long-life inoculant. The seeds were sown in pots containing soils from two crop areas. The second experiment was carried out in a laboratory, using a completely randomized design in an 8 × 7 factorial arrangement, with four replications. The treatments consisted of the same eight treatments used in the greenhouse experiment, which were applied before packaging and storing the seeds for two months. The industrial seed treatment with the mixture of fungicide, insecticide, and inoculants improved the soybean photosynthesis and nodulation processes, regardless of the history of the area. Soybean seeds can be stored for commercial purposes for up to 40 days, regardless of the seed treatment with fungicide, insecticide, and long-life inoculant applied alone or in combination.
Hemp (Cannabis sativa L.) Tolerates Chelator Stress Showing Varietal Differences and Concentration Dependence
Authors: Liu Feihu , Hu Jianming , Zhang Yating , Li Xuan , Yang Yang , Du Guanghui , Tang Kailei
Published: September 2023

The world’s arable land has been contaminated by heavy metals to a large extent, which has led to the decreasing availability of farmland. Thus, the remediation of heavy metal pollution deserves due attention, and phytoremediation is preferred. Hemp has been proposed as an ideal alternative crop for remediating heavy-metal-contaminated soil, owing to its well-developed roots, large biomass, and tolerance to heavy metals. Chelators can activate heavy metals to enhance plant absorption, but they may cause stress to plant growth. Therefore, it is very important to optimize the combination of chelator and plant (cultivar) for efficacious phytoremediation. The effects of different concentrations (2, 5, 10, 15, and 25 mmol·L−1) of the chelators disodium ethylene diamine tetra-acetate (EDTA) and citric acid (CA) on the seed germination and plant growth of hemp cultivars were investigated. Triple application of increasing concentrations of EDTA or CA two days apart gradually reduced the germination potential, germination rate, radicle length, and embryonic shoot length of hemp seed, although 2 mmol·L−1 of CA could even promote seed germination. Distinct varietal differences were found in the response of hemp to chelator stress. Under the scheme of four-time uses one week apart, both chelators caused a concentration-dependent linear decrease in the plant height, stem diameter, and biomass of hemp plants, but the growth inhibition due to CA was relatively milder. This could be partially explained by the change in tested physiological indices in hemp leaf. In conclusion, 2 mmol·L−1 of CA helped with seed germination and was almost nontoxic to plant growth; cultivar BM was more tolerant to the chelators than cultivar Y1.
Finnish Farmers Feel They Have Succeeded in Adopting Cover Crops but Need Down-to-Earth Support from Research
Authors: Peltonen-Sainio Pirjo , Jauhiainen Lauri , Känkänen Hannu
Published: September 2023

In Finland, there is an ongoing adoption and learning process considering the cultivation of cover crops (CCs). The primary aim is to claim the benefits of CCs for agricultural production and ecosystems, which are both appreciated by Finnish farmers. A farmer survey with 1130 respondents was carried out to build an up-to-date understanding of how farmers have succeeded with CCs and whether they intend to continue with the use of CCs and to collect farmers’ views on knowledge gaps that should be filled by research or better knowledge sharing. The studied groups were farmers who had selected CCs as a registered measure in 2020 to receive agricultural payments. Data came from the Finnish Food Authority. Organic farmers were slightly more positive: they have had longer experience with CCs, but organic production is also more dependent on the ecosystem services provided by CCs. A high share of respondents agreed that their experiences with CCs have improved over time and were confident that CCs had become a permanent element of their production systems. Most of the farmers also agreed that the area under CCs would expand significantly in Finland and considered the cultivation of CCs as an effective measure to improve soil conditions. They often considered that challenges in adopting CCs were exaggerated and disagreed that bad experiences prevented them from expanding or continuing the use of CCs. The agricultural payment available for Finnish farmers to support the cultivation of CCs is quite reasonable (EUR 97 + EUR 50 per hectare) to compensate for any economic risks of CCs. Free word answers from the farmers highlighted research needs (in descending order) in the following areas: crop protection, sowing practices, the use of diverse CCs and their mixtures, and impacts on yield and profitability. Many of these are universal, i.e., have been reported elsewhere. Younger farmers (≤50 years) highlighted profitability, which is, in many European countries, a key barrier to the deployment of CCs. Farmers from the east and north regions, where the growing season is short, highlighted alternative CC choices as a knowledge gap.
Effect of Perineal Urethrostomy on the Length of the Urethra of the Cat: A Cadaveric Study
Authors: Shipov Anna , Israeli Inbar , Billet Jean-Philippe , Adam Yoav , Milgram Joshua
Published: September 2023

Perineal urethrostomy in cats is indicated for urethral pathologies located distal to the bulbourethral glands. The description of the bulbourethral glands as the cranial landmark when performing a PU is based on the increased urethral diameter at this location, rather than on an anatomical limitation. This suggests that urethral pathologies cranial to the bulbourethral glands could potentially be treated with PU. At present, the extent to which the pelvic urethra can be mobilized is unknown. Characterization and quantification of the effect of PU on the pelvic urethra is required prior to attempting to define the location of the pelvic urethra, cranial to the bulbourethral glands, which can be exteriorized when performing a PU. Our aim was to characterize and quantify the effect of performing a PU on the location and length of the pelvic urethra. Methods: Ten male feline cadavers were used, and four markers were placed on the pelvic urethra via a ventral approach to the peritoneal and pelvic cavities. Two orthogonal radiographic views were acquired prior and subsequent to performing a PU. The distance of each marker to a predefined landmark/origin and the distances of the markers relative to each other were measured on all radiographs. Results: PU resulted in significant caudal translation of the markers relative to the predefined landmark on all radiographic views; however, PU did not result in a significant change in the distances between the markers. Conclusions: Performing a PU results in caudal translation and minimal stretching of the mobilized pelvic urethra.
Migration Route of Sthenoteuthis oualaniensis in the South China Sea Based on Statolith Trace Element Information
Authors: Fan Jiangtao , Fang Zhou , Ma Shengwei , Zhang Peng , Feng Xue , Chen Zuozhi
Published: September 2023

Sthenoteuthis oualaniensis (Lesson, 1830) is a pelagic species with a complex population structure and wide migration range. The trace elements in statoliths are effective indicators for reconstructing the life history of an individual. In this study, the trace elements in statoliths were determined via laser ablation inductively coupled plasma mass spectrometry, and a multiple regression tree (MRT) model was used to trace the migration of S. oualaniensis and identify its potential habitats in the South China Sea. Na, Mg, Fe, Sr, and Ba were the effective trace elements, with significant differences found among stocks (p < 0.05). The MRT was divided into five clusters representing five life history stages. The Mg:Ca and Sr:Ca ratios decreased initially and increased thereafter, and the Mg:Ca, Sr:Ca, and Ba:Ca ratios differed significantly among the stages of the life history in each stock (p < 0.05). The hatching water temperatures for the winter and summer–autumn spawning populations were 28.05–28.88 °C (temperature at 25 m) and 27.15–27.92 °C (temperature at 25 m). The winter stock hatched in the southern South China Sea, and the larvae then migrated northwest during the summer monsoon. The summer–autumn stocks hatched in the northern South China Sea, and the larvae migrated southward under the mesoscale closed anticyclonic circulation in the northern South China Sea. These results provide insight into the migration of S. oualaniensis in the South China Sea.
Salmonella Control in Fattening Pigs through the Use of Esterified Formic Acid in Drinking Water Shortly before Slaughter
Authors: Bernad-Roche María , Marín-Alcalá Clara María , Vico Juan Pablo , Mainar-Jaime Raúl Carlos
Published: September 2023

The presence of Salmonella in pig feces is a major source of abattoir and carcass contamination, and one of the main sources of human salmonellosis. This study assessed whether using a form of esterified formic acid (30% formic acid) in drinking water (10 kg/1000 L) 5 days before slaughter could be a helpful strategy to mitigate this public health issue. Thus, 240 pigs from three Salmonella-positive commercial fattening farms were selected. From each farm, 40 pigs were allocated to a control group (CG) and 40 to a treatment group (TG). At the abattoir, fecal samples from both groups were collected for Salmonella detection (ISO 6579-1:2017) and quantification (ISO/TS 6579-2:2012). Salmonella was present in 35% (95% IC = 29.24–41.23) of the samples collected. The prevalence was significantly higher in the CG than in the TG (50% vs. 20%; p < 0.001). In all farms, the TG showed a lower percentage of shedders than the CG. A random-effects logistic model showed that the odds of shedding Salmonella were 5.63 times higher (95% CI = 2.92–10.8) for the CG than for the TG. Thus, the proportion of pigs shedding Salmonella that was prevented in the TG due to the use of this form of organic acid was 82.2%. In addition, a Chi-squared analysis for trends showed that the higher the Salmonella count, the higher the odds of the sample belonging to the CG. These results suggest that adding this type of acid to drinking water 5 days before slaughter could reduce the proportion of Salmonella-shedding pigs and the Salmonella loads in the guts of shedder pigs.
Acclimation and Blood Sampling: Effects on Stress Markers in C57Bl/6J Mice
Authors: Marin Nerea , Moragon Amparo , Gil Domingo , Garcia-Garcia Francisco , Bisbal Viviana
Published: September 2023

Blood sampling in rodents is common practice in scientific studies. Some of the refined methods widely used are the puncture of the saphenous vein or tail vein, or even tail docking. The handling needs of these different blood sampling methods are different and can directly affect stress, increasing the variability of the study. Moreover, there is less aversion and stress if the animal is accustomed to the environment, handling and technique. Therefore, our study aimed to assess the influence of these three blood sampling techniques (saphenous puncture, tail vein puncture and tail vein docking) and the use of previous acclimation on different indicators of animal stress, assessing blood glucose concentrations and faecal corticosterone metabolites (FCMs). Twenty-four young adult male and female C57Bl6/J mice were divided in three groups by sampling method: tail docking (TD), saphenous vein puncture (SV) and caudal vein puncture (CV) groups. All mice were studied with and without acclimation, which was performed during 9 consecutive days. The results showed that both males and females present very similar responses to the different handling and sampling methods without significant differences. Nevertheless, acclimation in all sampling methods decreased glucose and FCM levels significantly. The method that obtained the lowest glucose and FCM levels with significance was saphenous vein puncture. Therefore, we can say that it causes less stress when performing prior acclimation, even when this involves greater handling of the animal. Our results contribute to refinement within the 3R concept and could serve researchers to programme and select a good handling technique and a welfare-friendly blood sampling method for their experiments.
African Swine Fever Shock: China’s Hog Industry’s Resilience and Its Influencing Factors
Authors: Shi Zizhong , Hu Xiangdong
Published: September 2023

African swine fever has damaged the foundation of China’s hog industry, caused a serious decline in hog production, highlighted the contradiction between supply and demand in the pork market, and led to major economic and social impacts. The industrial resilience of 31 Chinese provinces to African swine fever shock and its spatial and temporal differentiation characteristics from 2018 to 2021 were measured in this study from the two dimensions of resistance and recoverability. Using Geodetector, the key factors influencing the resilience of China’s hog industry were explored. The results showed that 2018–2019 and 2020–2021 represented the resistance and recovery periods of the hog industry under African swine fever shock, respectively, with poor resilience characterizing the resistance period and improved resilience characterizing the recovery period. At the early stages of the African swine fever outbreak, the hog industries in Tianjin, Shanxi, Guangxi, and Yunnan had robust resistance due to the slaughter rate, economic level, mortality rate, carcass weight, and culling rate in those areas. At the most severe stage of the outbreak, resistance was generally poor in all provinces due to the slaughter rate, per capita consumption, and scale level at the time. During the period of rapid recovery in hog production, the recoverability of each province was very strong due to the industrial structure, culling rate, economic level, and resource carrying capacity at that time. During the reasonable adjustment period of hog production capacity, the recoverability based on the breeding sow inventory in 13 provinces, including Henan, Shandong, and other large hog-breeding provinces, was negative due to the scale level, slaughter rate, per capita consumption, and resource carrying at that time. Taking measures to enhance the resilience of the hog industry, strengthen the prevention and control of hog epidemics, improve the monitoring and early warning mechanisms, and enhance the ability of the hog industry to cope with major animal epidemics is recommended.
Thermal Response of Laboratory Rats (Rattus norvegicus) during the Application of Six Methods of Euthanasia Assessed by Infrared Thermography
Authors: Domínguez-Oliva Adriana , Hernández-Ávalos Ismael , Olmos-Hernández Adriana , Villegas-Juache Juan , Verduzco-Mendoza Antonio , Mota-Rojas Daniel
Published: September 2023

Refinement is one of the principles aiming to promote welfare in research animals. The techniques used during an experimental protocol, including euthanasia selection, must prevent and minimize suffering. Although the current euthanasia methods applied to laboratory rodents are accepted, the controversial findings regarding the potential stress/distress they can cause is a field of research. The objective was to assess the thermal response of Wistar rats during various euthanasia methods using infrared thermography (IRT) to determine the method that prevents or diminishes the stress response and prolonged suffering. Pentobarbital (G1), CO2 (G2), decapitation (G3), isoflurane (G4), ketamine + xylazine (G5), and ketamine + CO2 (G6) were evaluated at five evaluation times with IRT to identify changes in the surface temperature of four anatomical regions: ocular (T°ocu), auricular (T°ear), interscapular (T°dor), and caudal (T°tai). Significant differences (p < 0.05) were found in G2 and G4, registering temperature increases from the administration of the drug to the cessation of respiratory rate and heart rate. Particularly, isoflurane showed a marked thermal response in T°ocu, T°ear, T°dor, and T°tai, suggesting that, in general, inhalant euthanasia methods induce stress in rats and that isoflurane might potentially cause distress, an effect that must be considered when deciding humane euthanasia methods in laboratory rodents.
Generation of Antibodies Selectively Recognizing Epitopes in a Formaldehyde-Fixed Cell-Surface Antigen Using Virus-like Particle Display and Hybridoma Technology
Authors: Schatz Stefanie , Willnow Lena , Winkels Monika , Rosengarten Jamila Franca , Theek Benjamin , Johnston Ian C. D. , Stitz Jörn
Published: September 2023

Efficient induction of target-specific antibodies can be elicited upon immunization with highly immunogenic virus-like particles (VLPs) decorated with desired membrane-anchored target antigens (Ags). However, for example, for diagnostic purposes, monoclonal antibodies (mAbs) are required to enable the histological examination of formaldehyde-fixed paraffin-embedded (FFPE) biopsy tissue samples. Aiming at the generation of FFPE-antigen-specific mAbs and as a proof of concept (POC), we first established a simplified protocol using only formaldehyde and 90 °C heat fixation (FF90) of cells expressing the target Ag nerve growth factor receptor (NGFR). The FF90 procedure was validated using flow cytometric analysis and two mAbs recognizing either the native and FFPE-Ag or exclusively the native Ag. C-terminally truncated NGFR (trNGFR)-displaying native and FF90-treated VLPs derived from HIV-1 did not reveal distinctive changes in particle morphology using transmission electron microscopy (TEM) and dynamic light scattering (DLS) analysis. Mice were subsequently repetitively immunized with trNGFR-decorated FF90-VLPs and hybridoma technology was used to establish mAb-producing cell clones. In multiple screening rounds, nine cell clones were identified producing mAbs distinctively recognizing epitopes in FF90- and FFPE-NGFR. This POC of a new methodology should foster the future generation of mAbs selectively targeting FFPE-fixed cell-surface Ags.
The Visibility of Changes in the Antioxidant Compound Profiles of Strawberry and Raspberry Fruits Subjected to Different Storage Conditions Using ATR-FTIR and Chemometrics
Authors: Sachadyn-Król Monika , Budziak-Wieczorek Iwona , Jackowska Izabella
Published: September 2023

Strawberry cultivars Portola and Enduro, as well as raspberry cultivars Enrosadira and Kwazi, were evaluated for their antioxidant potential after treatment with gaseous ozone and different refrigeration storage conditions. Their antioxidant capacity was investigated with ABTS and DPPH methods, and the chemical composition was determined by measuring the total phenolic (TPC) and flavonoid (TFC) compounds. The classification of different samples of berry puree was influenced significantly by both the cultivars and the refrigeration storage method. Moreover, FTIR spectroscopy coupled with chemometrics was used as an alternative technique to conventional methods to determine the chemical composition of strawberries and raspberries. The chemometric discrimination of samples was achieved using principal component analysis (PCA), hierarchical clustering analysis (HCA) and linear discriminant analysis (LDA) modelling procedures performed on the FTIR preprocessed spectral data for the fingerprint region (1800–500 cm−1). The fingerprint range between 1500 and 500 cm−1, corresponding to deformation vibrations from polysaccharides, pectin and organic acid content, had a significant impact on the grouping of samples. The results obtained by PCA-LDA scores revealed a clear separation between four classes of samples and demonstrated a high overall classification rate of 97.5% in differentiating between the raspberry and strawberry cultivars.
Effects of Dietary Supplementation of a Resin-Purified Aqueous-Isopropanol Olive Leaf Extract on Meat and Liver Antioxidant Parameters in Broilers
Authors: Vasilopoulou Konstantina , Papadopoulos Georgios A. , Lioliopoulou Styliani , Pyrka Ioanna , Nenadis Nikolaos , Savvidou Soumela , Symeon George , Dotas Vassilios , Panitsidis Ioannis , Arsenos Georgios , Giannenas Ilias
Published: September 2023

Olive leaves are byproducts οf the agro-industrial sector and are rich in bioactive compounds with antioxidant properties. They could be supplemented in poultry diets powdered or less frequently as extracts to improve performance, health and product quality. The objective of this study was to investigate the possible beneficial effects of an aqueous isopropanol olive leaf extract—purified through filtration (250–25 µm) and a resin (XAD-4)—when supplemented in broiler chickens’ diets, on meat quality parameters, focusing mainly on antioxidant parameters as there is limited published information. For this purpose, four-hundred-and-eighty-day-old broilers were randomly assigned to four dietary treatments: T1 (control: basal diet); T2 (1% olive leaf extract); T3 (2.5% olive leaf extract); T4 (positive control: 0.1% encapsulated oregano oil commercially used as feed additive). At the end of the experimental period (day 42), the birds were slaughtered, and samples from breast, thigh meat and liver were collected for antioxidant parameters evaluation. On day 1, after slaughter, in thigh meat, Malondialdehyde (MDA) was lower in T2 compared to T3, and total phenolic content (TPC) was higher in T2 compared to T3 and T4. Total antioxidant capacity (TAC) was increased in T2 and T4 breast meat compared to the control. In liver, T4 treatment resulted in higher TPC. The lack of dose-dependent effect for olive leaf extract may be attributed to the pro-oxidant effects of some bioactive compounds found in olive leaves, such as oleuropein, when supplemented at higher levels. In summary, it can be inferred that the inclusion of 1% olive leaf extract in the feed of broilers has the potential to mitigate oxidation in broiler meat and maybe enhance its quality.
Novel Kefir Exopolysaccharides (KEPS) Mitigate Lipopolysaccharide (LPS)-Induced Systemic Inflammation in Luciferase Transgenic Mice through Inhibition of the NF-κB Pathway
Authors: Liao Chun-Huei , Yen Chih-Ching , Chen Hsiao-Ling , Liu Yu-Hsien , Chen Yu-Hsuan , Lan Ying-Wei , Chen Ke-Rong , Chen Wei , Chen Chuan-Mu
Published: September 2023

A novel kefir exopolysaccharides (KEPS) derived from kefir grain fermentation were found to have a small molecular weight (12 kDa) compared to the traditionally high molecular weight (12,000 kDa) of kefiran (KE). KE has been shown to possess antioxidant, blood pressure-lowering, and immune-modulating effects. In this study, we characterized KEPS and KE and evaluated their anti-inflammatory properties in vitro using RAW264.7 macrophages. The main monosaccharide components were identified as glucose (98.1 ± 0.06%) in KEPS and galactose (45.36 ± 0.16%) and glucose (47.13 ± 0.06%) in KE, respectively. Both KEPS and KE significantly reduced IL-6 secretion in lipopolysaccharide (LPS)-stimulated macrophages. We further investigated their effects in LPS-induced systemic injury in male and female NF-κB-luciferase+/+ transgenic mice. Mice received oral KEPS (100 mg/kg) or KE (100 mg/kg) for seven days, followed by LPS or saline injection. KEPS and KE inhibited NF-κB signaling, as indicated by reduced luciferase expression and phosphorylated NF-κB levels. LPS-induced systemic injury increased luciferase signals, especially in the kidney, spleen, pancreas, lung, and gut tissues of female mice compared to male mice. Additionally, it upregulated inflammatory mediators in these organs. However, KEPS and KE effectively suppressed the expression of inflammatory mediators, including p-MAPK and IL-6. These findings demonstrate that KEPS can alleviate LPS-induced systemic damage by inhibiting NF-κB/MAPK signaling, suggesting their potential as a treatment for inflammatory disorders.
Hide45: A Method for Optimal Payload Data Hiding in Base45 Encoded Strings
Authors: Botta Marco , Cavagnino Davide , Druetto Alessandro
Published: September 2023

Base45 encodes pairs of octets using 3 characters from an alphabet of 45 printable symbols. Previous works showed the ability to hide payload data into encoded strings by exploiting the unused configurations of the Base45 encoding. In this paper, we present Hide45, an algorithm for hiding data into Base45 encoded strings that optimizes the embedding capacity, according to the frequency distribution of the input data. Experimental tests show that an optimal assignment of bit configurations to the most frequent pairs of octets allows to reach a payload capacity very close to the theoretical capacity of the method, improving over a baseline assignment by up to 53%.