bluuebunny/crossref_metadata_2025 huggingface dataset:
Special Staining and Protein Expression of VEGF/EGFR and P53/NF-κB in Cryptorchid Tissue of Erhualian Pigs
Authors: Liu Penggang , Shao Yiming , Liu Caihong , Lv Xiaoyang , Afedo Seth Yaw , Bao Wenbin
Published: January 2024

Erhualian pigs exhibit one of the highest reproductive rates globally, and cryptorchidism is a crucial factor affecting reproductive abilities of boars. This investigation focused on cryptorchid tissues from Erhualian pigs, where the histological structure of cryptorchidism was observed using specialized staining. In addition, protein expression of P53/NF-κB in cryptorchid tissues was assessed using Western blot and immunohistochemistry. In comparison to normal Erhualian testes, Masson’s trichrome staining indicated a reduction in collagen fibers in the connective tissue and around the basal membrane of the seminiferous tubules in cryptorchid testes. Moreover, collagen fiber distribution was observed to be disordered. Verhoeff Van Gieson (EVG) and argyrophilic staining demonstrated brownish-black granular nucleoli organized regions in mesenchymal cells and germ cells. When compared to normal testicles, the convoluted seminiferous tubules of cryptorchids exhibited a significantly reduced number and diameter (p < 0.01). Notably, VEGF/EGFR and P53/NF-κB expression in cryptorchidism significantly differed from that in normal testes. In particular, the expression of VEGF and P53 in cryptorchid tissues was significantly higher than that in normal testes tissues, whereas the expression of EGFR in cryptorchid tissues was significantly lower than that in normal testes tissues (all p < 0.01). NF-κB expressed no difference in both conditions. The expressions of VEGF and NF-κB were observed in the cytoplasm of testicular Leydig cells and spermatogenic cells, but they were weak in the nucleus. EGFR and P53 were more positively expressed in the cytoplasm of these cells, with no positive expression in the nucleus. Conclusion: There were changes in the tissue morphology and structure of the cryptorchid testis, coupled with abnormally high expression of VEGF and P53 proteins in Erhualian pigs. We speculate that this may be an important limiting factor to fecundity during cryptorchidism.
Efficient Photocatalytic Luminous Textile for Simulated Real Water Purification: Advancing Economical and Compact Reactors
Authors: Assadi Amin Aymen
Published: January 2024

The growing worldwide problem of wastewater management needs sustainable methods for conserving water supplies while addressing environmental and economic considerations. With the depletion of freshwater supplies, wastewater treatment has become critical. An effective solution is needed to efficiently treat the organic contaminants departing from wastewater treatment plants (WWTPs). Photocatalysis appears to be a viable method for eliminating these recalcitrant micropollutants. This study is focused on the degradation of Reactive Black 5 (RB5), a typical contaminant from textile waste, using a photocatalytic method. Titanium dioxide (TiO2) was deposited on a novel luminous fabric and illuminated using a light-emitting diode (LED). The pollutant degrading efficiency was evaluated for two different light sources: (i) a UV lamp as an external light source and (ii) a cold LED. Interestingly, the LED UV source design showed more promising results after thorough testing at various light levels. In fact, we note a 50% increase in mineralization rate when we triple the number of luminous tissues in the same volume of reactor, which showed a clear improvement with an increase in compactness.
Regulated Phase Separation in Al–Ti–Cu–Co Alloys through Spark Plasma Sintering Process
Authors: Lee Seulgee , Chokradjaroen Chayanaphat , Sawada Yasuyuki , Yoon Sungmin , Saito Nagahiro
Published: January 2024

With the goal of developing lightweight Al-Ti-containing multicomponent alloys with excellent mechanical strength, an Al–Ti–Cu–Co alloy with a phase-separated microstructure was prepared. The granulometry of metal particles was reduced using planetary ball milling. The particle size of the metal powders decreased as the ball milling time increased from 5, 7, to 15 h (i.e., 6.6 ± 6.4, 5.1 ± 4.3, and 3.2 ± 2.1 μm, respectively). The reduction in particle size and the dispersion of metal powders promoted enhanced diffusion during the spark plasma sintering process. This led to the micro-phase separation of the (Cu, Co)2AlTi (L21) phase, and the formation of a Cu-rich phase with embedded nanoscale Ti-rich (B2) precipitates. The Al–Ti–Cu–Co alloys prepared using powder metallurgy through the spark plasma sintering exhibited different hardnesses of 684, 710, and 791 HV, respectively, while maintaining a relatively low density of 5.8–5.9 g/cm3 (<6 g/cm3). The mechanical properties were improved due to a decrease in particle size achieved through increased ball milling time, leading to a finer grain size. The L21 phase, consisting of (Cu, Co)2AlTi, is the site of basic hardness performance, and the Cu-rich phase is the mechanical buffer layer between the L21 and B2 phases. The finer network structure of the Cu-rich phase also suppresses brittle fracture.
Reactivity of Calcined Clays as SCM—A Review
Authors: Overmann Steffen , Vollpracht Anya , Matschei Thomas
Published: January 2024

Calcined mixed clays are one of the most promising alternative supplementary cementitious materials. However, their standardized use is difficult due to the wide range of compositions of the raw materials. The reactivity potential of different clays can hardly be estimated on the basis of simple characteristics so far. This review aims to identify and compile the factors that determine reactivity. At first, an overview of the methods to evaluate reactivity is presented in order to provide a definition of this term. Subsequently, the reactivity-determining factors are compiled and subdivided into the characteristics of the raw material (chemical and mineralogical composition), the parameters of calcination (furnace type, temperature, grain size, retention time, and cooling), and the characteristics of the calcined material (physical properties and amorphous phase). Interrelations are discussed qualitatively. In the second step, a quantitative literature analysis was conducted to quantify correlations between the different factors and reactivity. However, since the characterization methods in the literature are very different, the data can hardly be analyzed quantitatively. Consequently, this paper points out what information is needed to conduct profound, comparable studies to evaluate the reactivity potential of clays.
Fatigue Life Prediction for Injection-Molded Carbon Fiber-Reinforced Polyamide-6 Considering Anisotropy and Temperature Effects
Authors: Choi Joeun , Andrian Yohanes Oscar , Lee Hyungtak , Lee Hyungyil , Kim Naksoo
Published: January 2024

The effects of anisotropy and temperature of short carbon fiber-reinforced polyamide-6 (CF-PA6) by the injection molding process were investigated to obtain the static and fatigue characteristics. Static and fatigue tests were conducted with uniaxial tensile and three-point bending specimens with various fiber orientations at temperatures of 40, 60, and 100 °C. The anisotropy caused by the fiber orientations along a polymer flow was calculated using three software connecting analysis sequences. The characteristics of tensile strength and fatigue life can be changed by temperature and anisotropy variations. A semi-empirical strain–stress fatigue life prediction model was proposed, considering cyclic and thermodynamic properties based on the Arrhenius equation. The developed model had a good agreement with an R2 = 0.9457 correlation coefficient. The present fatigue life prediction of CF-PA6 can be adopted when designers make suitable decisions considering the effects of temperature and anisotropy.
Polymersomes as the Next Attractive Generation of Drug Delivery Systems: Definition, Synthesis and Applications
Authors: Fonseca Mariana , Jarak Ivana , Victor Francis , Domingues Cátia , Veiga Francisco , Figueiras Ana
Published: January 2024

Polymersomes are artificial nanoparticles formed by the self-assembly process of amphiphilic block copolymers composed of hydrophobic and hydrophilic blocks. They can encapsulate hydrophilic molecules in the aqueous core and hydrophobic molecules within the membrane. The composition of block copolymers can be tuned, enabling control of characteristics and properties of formed polymersomes and, thus, their application in areas such as drug delivery, diagnostics, or bioimaging. The preparation methods of polymersomes can also impact their characteristics and the preservation of the encapsulated drugs. Many methods have been described, including direct hydration, thin film hydration, electroporation, the pH-switch method, solvent shift method, single and double emulsion method, flash nanoprecipitation, and microfluidic synthesis. Considering polymersome structure and composition, there are several types of polymersomes including theranostic polymersomes, polymersomes decorated with targeting ligands for selective delivery, stimuli-responsive polymersomes, or porous polymersomes with multiple promising applications. Due to the shortcomings related to the stability, efficacy, and safety of some therapeutics in the human body, polymersomes as drug delivery systems have been good candidates to improve the quality of therapies against a wide range of diseases, including cancer. Chemotherapy and immunotherapy can be improved by using polymersomes to deliver the drugs, protecting and directing them to the exact site of action. Moreover, this approach is also promising for targeted delivery of biologics since they represent a class of drugs with poor stability and high susceptibility to in vivo clearance. However, the lack of a well-defined regulatory plan for polymersome formulations has hampered their follow-up to clinical trials and subsequent market entry.
New Method for Precise Measurement of Clamping Plate Deformations on Forming Presses
Authors: Ivanov Georg , Burkhardt Thomas , Penter Lars , Ihlenfeldt Steffen
Published: January 2024

The deformation of press bolster plates under load can significantly impact the quality of manufactured workpieces. Consequently, press manufacturers are usually obliged to provide the metric proof of the permissible deformation values during the press commissioning process. Unfortunately, the existing measurement methods for determining bolster plate deformations present flaws in the measurement accuracy, the application flexibility, the metrological effort, and other aspects. These issues have been addressed through the development of a new measurement method using multiple inclination profiles on the surface of the measured object. Based on the difference in the inclination between the unloaded and loaded states, the method approximates the inclination surface and calculates the deformation of the measured object through integration. The measurement method was first used for commissioning tests of forming presses. A comparison with the results obtained with a measurement frame equipped with high-resolution measurement probes demonstrates an accuracy of the new method of less than 20 µm.
Evaluating the Discrete Generalized Rayleigh Distribution: Statistical Inferences and Applications to Real Data Analysis
Authors: Haj Ahmad Hanan , Ramadan Dina A. , Almetwally Ehab M.
Published: January 2024

Various discrete lifetime distributions have been observed in real data analysis. Numerous discrete models have been derived from a continuous distribution using the survival discretization method, owing to its simplicity and appealing formulation. This study focuses on the discrete analog of the newly generalized Rayleigh distribution. Both classical and Bayesian statistical inferences are performed to evaluate the efficacy of the new discrete model, particularly in terms of relative bias, mean square error, and coverage probability. Additionally, the study explores different important submodels and limiting behavior for the new discrete distribution. Various statistical functions have been examined, including moments, stress–strength, mean residual lifetime, mean past time, and order statistics. Finally, two real data examples are employed to evaluate the new discrete model. Simulations and numerical analyses play a pivotal role in facilitating statistical estimation and data modeling. The study concludes that the discrete generalized Rayleigh distribution presents a notably appealing alternative to other competing discrete distributions.
Fractional Boundary Layer Flow: Lie Symmetry Analysis and Numerical Solution
Authors: Jannelli Alessandra , Speciale Maria Paola
Published: January 2024

In this paper, we present a fractional version of the Sakiadis flow described by a nonlinear two-point fractional boundary value problem on a semi-infinite interval, in terms of the Caputo derivative. We derive the fractional Sakiadis model by substituting, in the classical Prandtl boundary layer equations, the second derivative with a fractional-order derivative by the Caputo operator. By using the Lie symmetry analysis, we reduce the fractional partial differential equations to a fractional ordinary differential equation, and, then, a finite difference method on quasi-uniform grids, with a suitable variation of the classical L1 approximation formula for the Caputo fractional derivative, is proposed. Finally, highly accurate numerical solutions are reported.
A Bioinspired Test Generation Method Using Discretized and Modified Bat Optimization Algorithm
Authors: Arasteh Bahman , Arasteh Keyvan , Kiani Farzad , Sefati Seyed Salar , Fratu Octavian , Halunga Simona , Tirkolaee Erfan Babaee
Published: January 2024

The process of software development is incomplete without software testing. Software testing expenses account for almost half of all development expenses. The automation of the testing process is seen to be a technique for reducing the cost of software testing. An NP-complete optimization challenge is to generate the test data with the highest branch coverage in the shortest time. The primary goal of this research is to provide test data that covers all branches of a software unit. Increasing the convergence speed, the success rate, and the stability of the outcomes are other goals of this study. An efficient bioinspired technique is suggested in this study to automatically generate test data utilizing the discretized Bat Optimization Algorithm (BOA). Modifying and discretizing the BOA and adapting it to the test generation problem are the main contributions of this study. In the first stage of the proposed method, the source code of the input program is statistically analyzed to identify the branches and their predicates. Then, the developed discretized BOA iteratively generates effective test data. The fitness function was developed based on the program’s branch coverage. The proposed method was implemented along with the previous one. The experiments’ results indicated that the suggested method could generate test data with about 99.95% branch coverage with a limited amount of time (16 times lower than the time of similar algorithms); its success rate was 99.85% and the average number of required iterations to cover all branches is 4.70. Higher coverage, higher speed, and higher stability make the proposed method suitable as an efficient test generation method for real-world large software.
Mixed-Mode Fracture Analysis of a Patterned Si Electrode during Lithiation/Delithiation
Authors: Gwak Yunki , Han Jang-Woo
Published: January 2024

We investigate the mechanical degradation of patterned Si based on large deformation theory and mixed-mode failure. Mode I debonding at the center of Si is found to suddenly increase and lead to crack initiation during the early stages of lithiation. The generated crack propagates to the surface and hinders Li diffusion, thus increasing the inhomogeneity of Li within Si. During delithiation, very little Mode I and Mode II debonding occur near the center of the patterned Si; however, both Mode I and Mode II debonding develop considerably at the surface. In addition, the effects of the charge/discharge rate are considered. A very low state of charge induces crack initiation at the center of Si, regardless of the charge rate. The charge/discharge rate is correlated with the total crack length, which is directly proportional to the charge rate. Based on our simulation results, we propose a new shape of the patterned Si with a hole in order to enhance mechanical stability. The hole prevents crack growth by releasing the internal stress, and Mode I debonding at the center of the patterned Si becomes significantly lower, with much slower increases during lithiation.
Optimal Error Quantification and Robust Tracking under Unknown Upper Bounds on Uncertainties and Biased External Disturbance
Authors: Sokolov Victor F.
Published: January 2024

This paper addresses a problem of optimal error quantification in the framework of robust control theory in the 𝓁1 setup. The upper bounds of biased external disturbance and the gains of coprime factor perturbations in a discrete-time linear time invariant SISO plant are assumed to be unknown. The computation of optimal data-consistent upper bounds under a known bias of external disturbance has been simplified to linear programming. This allows for the computation of optimal estimates in real-time and their application to achieve optimal robust steady-state tracking even when facing an unknown bias in the external disturbance. The presented results have been illustrated through computer simulations.
An Optimized LSTM Neural Network for Accurate Estimation of Software Development Effort
Authors: Iordan Anca-Elena
Published: January 2024

Software effort estimation has constituted a significant research theme in recent years. The more important provocation for project managers concerns reaching their targets within the fixed time boundary. Machine learning strategies can lead software management to an entire novel stage. The purpose of this research work is to compare an optimized long short-term memory neural network, based on particle swarm optimization, with six machine learning methods used to predict software development effort: K-nearest neighbours, decision tree, random forest, gradient boosted tree, multilayer perceptron, and long short-term memory. The process of effort estimation uses five datasets: China and Desharnais, for which outputs are expressed in person-hours; and Albrecht, Kemerer, and Cocomo81, for which outputs are measured in person-months. To compare the accuracy of these intelligent methods four metrics were used: mean absolute error, median absolute error, root mean square error, and coefficient of determination. For all five datasets, based on metric values, it was concluded that the proposed optimized long short-term memory intelligent method predicts more accurately the effort required to develop a software product. Python 3.8.12 programming language was used in conjunction with the TensorFlow 2.10.0, Keras 2.10.0, and SKlearn 1.0.1 to implement these machine learning methods.
Adaptive Normalization and Feature Extraction for Electrodermal Activity Analysis
Authors: Viana-Matesanz Miguel , Sánchez-Ávila Carmen
Published: January 2024

Electrodermal Activity (EDA) has shown great potential for emotion recognition and the early detection of physiological anomalies associated with stress. However, its non-stationary nature limits the capability of current analytical and detection techniques, which are highly dependent on signal stability and controlled environmental conditions. This paper proposes a framework for EDA normalization based on the exponential moving average (EMA) with outlier removal applicable to non-stationary heteroscedastic signals and a novel set of features for analysis. The normalized time series preserves the morphological and statistical properties after transformation. Meanwhile, the proposed features expand on typical time-domain EDA features and profit from the resulting normalized signal properties. Parameter selection and validation were performed using two different EDA databases on stress assessment, accomplishing trend preservation using windows between 5 and 20 s. The proposed normalization and feature extraction framework for EDA analysis showed promising results for the identification of noisy, relaxed and arousal-like patterns in data with conventional clustering approaches like K-means over the aforementioned normalized features.
Dissipative Fuzzy Filtering for Nonlinear Networked Systems with Dynamic Quantization and Data Packet Dropouts
Authors: Jing Shuxia , Lu Chengming , Li Zhimin
Published: January 2024

This paper discusses the dissipative filtering problem for discrete-time nonlinear networked systems with dynamic quantization and data packet dropouts. The Takagi–Sugeno (T–S) fuzzy model is employed to approximate the considered nonlinear plant. Both the measurement and performance outputs are assumed to be quantized by the dynamic quantizers before being transmitted. Moreover, the Bernoulli stochastic variables are utilized to characterize the effects of data packet dropouts on the measurement and performance outputs. The purpose of this paper is to design full- and reduced-order filters, such that the stochastic stability and dissipative filtering performance for the filtering error system can be guaranteed. The collaborative design conditions for the desired filter and the dynamic quantizers are expressed in the form of linear matrix inequalities. Finally, simulation results are used to illustrate the feasibility of the proposed filtering scheme.
Research on Optimization Method of Evaporation Duct Prediction Model
Authors: Cui Yingxue , Hu Tong , Qi Ke , Qiu Zhijin , Zou Jing , Li Zhiqian , Wang Bo
Published: January 2024

The sea surface roughness parameterization and the universal stability function are key components of the evaporation duct prediction model based on the Monin–Obukhov similarity theory. They determine the model’s performance, which in turn affects the efficiency and accuracy of electromagnetic applications at sea. In this study, we collected layered meteorological and hydrological observation data and preprocessed them to obtain near-surface reference modified refractivity profiles. We then optimized the sea surface roughness parameterization and the universal stability function using particle swarm optimization and simulated annealing algorithms. The results show that the particle swarm optimization algorithm outperforms the simulated annealing algorithm. Compared to the original model, the particle swarm optimization algorithm improved the prediction accuracy of the model by 5.09% under stable conditions and by 9.97% under unstable conditions, demonstrating the feasibility of the proposed method for optimizing the evaporation duct prediction model. Subsequently, we compared the electromagnetic wave propagation path losses under two different evaporation duct heights and modified refractivity profile states, confirming that the modified refractivity profile is more suitable as the accuracy criterion for the evaporation duct prediction model.
Idiopathic Slow Transit Constipation: Pathophysiology, Diagnosis, and Management
Authors: Vlismas Luke , Wu William , Ho Vincent
Published: January 2024

Slow transit constipation (STC) has an estimated prevalence of 2–4% of the general population, and although it is the least prevalent of the chronic constipation phenotypes, it more commonly causes refractory symptoms and is associated with significant psychosocial stress, poor quality of life, and high healthcare costs. This review provides an overview of the pathophysiology, diagnosis, and management options in STC. STC occurs due to colonic dysmotility and is thought to be a neuromuscular disorder of the colon. Several pathophysiologic features have been observed in STC, including reduced contractions on manometry, delayed emptying on transit studies, reduced numbers of interstitial cells of Cajal on histology, and reduced amounts of excitatory neurotransmitters within myenteric plexuses. The underlying aetiology is uncertain, but autoimmune and hormonal mechanisms have been hypothesised. Diagnosing STC may be challenging, and there is substantial overlap with the other clinical constipation phenotypes. Prior to making a diagnosis of STC, other primary constipation phenotypes and secondary causes of constipation need to be ruled out. An assessment of colonic transit time is required for the diagnosis and can be performed by a number of different methods. There are several different management options for constipation, including lifestyle, dietary, pharmacologic, interventional, and surgical. The effectiveness of the available therapies in STC differs from that of the other constipation phenotypes, and prokinetics often make up the mainstay for those who fail standard laxatives. There are few available management options for patients with medically refractory STC, but patients may respond well to surgical intervention. STC is a common condition associated with a significant burden of disease. It can present a clinical challenge, but a structured approach to the diagnosis and management can be of great value to the clinician. There are many therapeutic options available, with some having more benefits than others.
EUS-Guided Combined Injection Therapy as a Secondary Prophylaxis of Gastric Variceal Bleeding in a Patient Contraindicated for TIPS: Case Report
Authors: Asenov Krasimir , Dimov Rosen , Kraeva Maria , Basheva-Kraeva Yordanka
Published: January 2024

Background: Although bleeding from gastric varices is less observed than esophageal variceal bleeding (VB) (25% vs. 64%), it is associated with an exceedingly high mortality rate of up to 45%. Current guidelines suggest that endoscopic cyanoacrylate injection therapy (ECI) is the first-line treatment for gastric variceal bleeding (GVB). A major concern, however, is the possibility of embolic incidents, which are clinically evident in approximately 1% of cases. There are no guidelines for secondary prophylaxis of GVB. Radiological treatments using a transjugular intrahepatic portosystemic shunt (TIPS) or balloon occlusive transvenous obliteration (BRTO) are considered viable. However, they are not universally inapplicable; for instance, in the setting of pulmonary hypertension (TIPS). EUS-guided combined injection therapy (EUS-CIT) (embolization coils + cyanoacrylate) is an emerging procedure with a perceived reduced risk of systemic embolization. Case presentation: A patient with alcoholic liver cirrhosis was subjected to EUS-CIT as a secondary prophylaxis for GVB. He had three VB episodes of prior presentation treated by endoscopic band ligation (EBL) and ECI. Due to recurrent episodes of bleeding, he was referred to TIPS, but was considered contraindicated due to severe pulmonary hypertension. EUS-CIT was conducted with two embolization coils inserted into the varix, followed by an injection of 1.5 mL of cyanoacrylate glue. A 19 Ga needle, 0.035″ 14/70 mm coils, non-diluted n-butyl-caynoacrylate, and a transgastric approach were utilized. There were no immediate complications. Complete obliteration of the GV was observed in a follow-up endoscopy on day 30. Subsequent endoscopies in months three and six showed no progression of gastric varices. Conclusions: Our initial experience with EUS-CIT suggests that it can be successfully used as secondary prophylaxis for recurrent GVB.
Phage Lytic Protein CHAPSH3b Encapsulated in Niosomes and Gelatine Films
Authors: Marchianò Verdiana , Duarte Ana Catarina , Agún Seila , Luque Susana , Marcet Ismael , Fernández Lucía , Matos María , Blanco Mª del Carmen , García Pilar , Gutiérrez Gemma
Published: January 2024

Antimicrobial resistance (AMR) has emerged as a global health challenge, sparking worldwide interest in exploring the antimicrobial potential of natural compounds as an alternative to conventional antibiotics. In recent years, one area of focus has been the utilization of bacteriophages and their derivative proteins. Specifically, phage lytic proteins, or endolysins, are specialized enzymes that induce bacterial cell lysis and can be efficiently produced and purified following overexpression in bacteria. Nonetheless, a significant limitation of these proteins is their vulnerability to certain environmental conditions, which may impair their effectiveness. Encapsulating endolysins in vesicles could mitigate this issue by providing added protection to the proteins, enabling controlled release, and enhancing their stability, particularly at temperatures around 4 °C. In this work, the chimeric lytic protein CHAPSH3b was encapsulated within non-ionic surfactant-based vesicles (niosomes) created using the thin film hydrating method (TFH). These protein-loaded niosomes were then characterized, revealing sizes in the range of 30–80 nm, zeta potentials between 30 and 50 mV, and an encapsulation efficiency (EE) of 50–60%. Additionally, with the objective of exploring their potential application in the food industry, these endolysin-loaded niosomes were incorporated into gelatine films. This was carried out to evaluate their stability and antimicrobial efficacy against Staphylococcus aureus.
Comparative Genome Analysis of Polar Mesorhizobium sp. PAMC28654 to Gain Insight into Tolerance to Salinity and Trace Element Stress
Authors: Khanal Anamika , Han So-Ra , Lee Jun Hyuck , Oh Tae-Jin
Published: January 2024

In this study, Mesorhizobium sp. PAMC28654 was isolated from a soil sample collected from the polar region of Uganda. Whole-genome sequencing and comparative genomics were performed to better understand the genomic features necessary for Mesorhizobium sp. PAMC28654 to survive and thrive in extreme conditions and stresses. Additionally, diverse sequence analysis tools were employed for genomic investigation. The results of the analysis were then validated using wet-lab experiments. Genome analysis showed trace elements’ resistant proteins (CopC, CopD, CzcD, and Acr3), exopolysaccharide (EPS)-producing proteins (ExoF and ExoQ), and nitrogen metabolic proteins (NarG, NarH, and NarI). The strain was positive for nitrate reduction. It was tolerant to 100 mM NaCl at 15 °C and 25 °C temperatures and resistant to multiple trace elements (up to 1 mM CuSO4·5H2O, 2 mM CoCl2·6H2O, 1 mM ZnSO4·7H2O, 0.05 mM Cd(NO3)2·4H2O, and 100 mM Na2HAsO4·7H2O at 15 °C and 0.25 mM CuSO4·5H2O, 2 mM CoCl2·6H2O, 0.5 mM ZnSO4·7H2O, 0.01 mM Cd(NO3)2·4H2O, and 100 mM Na2HAsO4·7H2O at 25 °C). This research contributes to our understanding of bacteria’s ability to survive abiotic stresses. The isolated strain can be a potential candidate for implementation for environmental and agricultural purposes.
Chemical Constituents of Halophyte Suaeda glauca and Their Therapeutic Potential for Hair Loss
Authors: Kim Yun-Na , Park Min-Gyu , Kim Yu-Jung , Lee Jae-Sun , Kwon Bong-Oh , Rho Jung-Rae , Jeong Eun-Ju
Published: January 2024

Suaeda glauca, a halophyte in the Amaranthaceae family, exhibits remarkable resilience to high salt and alkali stresses despite the absence of salt glands or vesicles in its leaves. While there is growing pharmacological interest in S. glauca, research on its secondary metabolites remains limited. In this study, chemical constituents of the aerial parts of S. glauca were identified using 1D- and 2D-NMR experiments, and its biological activity concerning hair loss was newly reported. Eight compounds, including alkaloids (1~3), flavonoids (4~6), and phenolics (7 and 8), were isolated. The compounds, except the flavonoids, were isolated for the first time from S. glauca. In the HPLC chromatogram, quercetin-3-O-β-d-glucoside, kaempferol-3-O-β-d-glucoside, and kaempferol were identified as major constituents in the extract of S. glauca. Additionally, the therapeutic potential of the extract of S. glauca and the isolated compounds 1~8 on the expressions of VEGF and IGF-1, as well as the regulation of Wnt/β-catenin signaling, were evaluated in human follicle dermal papilla cells (HFDPCs) and human umbilical vein endothelial cells (HUVECs). Among the eight compounds, compound 4 was the most potent in terms of increasing the expression of VEGF and IGF-1 and the regulation of Wnt/β-catenin. These findings suggest that S. glauca extract and its compounds are potential new candidates for preventing or treating hair loss.
Effect of Oat Fiber Preparations with Different Contents of β-Glucan on the Formation of Acrylamide in Dietary Bread (Rusks)
Authors: Miśkiewicz Karolina , Rosicka-Kaczmarek Justyna , Kowalska Gabriela , Maher Agnieszka , Oracz Joanna
Published: January 2024

In the literature, there are few reports indicating hydrocolloids as a factor capable of reducing the amount of acrylamide formed in food. Therefore, the aim of the study was to examine the ability of soluble oat fiber to reduce the amount of acrylamide formed in the process of obtaining rusks. The effect of the concentration of β-glucans in oat fiber preparations at 20% and 30% and the amount of preparations used at 10%, 15%, and 20% was investigated. On the basis of the obtained test results, it was shown that the most optimal concentration of oat fiber preparation in rusks recipe is at 15%, regardless of the content of β-glucan in it. This concentration makes it possible to reduce the amount of acrylamide formed in baked goods and rusks by ~70% and ~60%, respectively, while maintaining the desired physical and chemical properties of the product. In addition, it was shown that the browning index and water activity strongly correlate with the content of acrylamide in rusks, which makes them good markers of this compound in rusks. The use of hydrocolloids in the form of oat fiber preparations with different contents of β-glucan as a tool for reducing the amount of acrylamide in rusks, at the same time, offers the possibility of enriching these products with a soluble dietary fiber with health properties.
Co-Immobilization of Laccase and Mediator into Fe-Doped ZIF-8 Significantly Enhances the Degradation of Organic Pollutants
Authors: Li Zixuan , Shi Qinghong , Dong Xiaoyan , Sun Yan
Published: January 2024

Co-immobilization of laccase and mediator 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) for wastewater treatment could simultaneously achieve the reusability of laccase and avoid secondary pollution caused by the toxic ABTS. Herein, Fe-induced mineralization was proposed to co-immobilize laccase and ABTS into a metal–organic framework (ZIF-8) within 30 min. Immobilized laccase (Lac@ZIF-8-Fe) prepared at a 1:1 mass ratio of Fe2+ to Zn2+ exhibited enhanced catalytic efficiency (2.6 times), thermal stability, acid tolerance, and reusability compared to free laccase. ABTS was then co-immobilized to form Lac+ABTS@ZIF-8-Fe (ABTS = 261.7 mg/g). Lac@ZIF-8-Fe exhibited significantly enhanced bisphenol A (BPA) removal performance over free laccase due to the local substrate enrichment effect and improved enzyme stability. Moreover, the Lac+ABTS@ZIF-8-Fe exhibited higher BPA removal efficiency than the free laccase+ABTS system, implying the presence of a proximity effect in Lac+ABTS@ZIF-8-Fe. In the successive malachite green (MG) removal, the MG degradation efficiency by Lac@ZIF-8-Fe was maintained at 96.6% at the fifth reuse with only an extra addition of 0.09 mM ABTS in each cycle. As for Lac+ABTS@ZIF-8-Fe, 58.5% of MG was degraded at the fifth cycle without an extra addition of ABTS. Taken together, this research has provided a novel strategy for the design of a co-immobilized laccase and ABTS system for the degradation of organic pollutants.
Heat Treatment Augments Antigen Detection of Dirofilaria immitis in Apparently Healthy Companion Dogs (3.8% to 7.3%): Insights from a Large-Scale Nationwide Survey across the United States
Authors: Barrantes Murillo Daniel Felipe , Moye Annie , Wang Chengming
Published: January 2024

Background: Heartworm disease (HWD) is a vector-borne disease caused by the filarial nematode Dirofilaria immitis. Low antigen levels caused by immune complex formation preclude HWD diagnosis. Heat treatment is an immune complex dissociation technique used to enhance antigen detection. Only a few studies have reported the benefits of heat treatment in nationwide surveys. Methods: To investigate the impact of heat treatment on the seroprevalence of HWD in companion dogs in the USA, serum samples (n = 3253) were analyzed for D. immitis antigen (DiroCHEK®, Zoetis) without and with heat treatment of the samples. Results: Compared to sera without heat treatment, heat treatment significantly increased overall prevalence from 3.8% (123/3253) to 7.3% (237/3253) (p < 10−4), expanding antigen detection from 32 to 39 of the 48 states and Washington District of Columbia included in this study. Conclusions: This study represents the largest nationwide survey of HW antigen detection in dogs in the US applying heat treatment to canine sera. The heat treatment used herein has the advantage of requiring a low volume of serum, making it optimal for use in routine diagnosis. Heat treatment should be used routinely by reference laboratories and veterinary clinics in patients with a negative initial test.
A Novel Combined Dry Powder Inhaler Comprising Nanosized Ketoprofen-Embedded Mannitol-Coated Microparticles for Pulmonary Inflammations: Development, In Vitro–In Silico Characterization, and Cell Line Evaluation
Authors: Banat Heba , Csóka Ildikó , Paróczai Dóra , Burian Katalin , Farkas Árpád , Ambrus Rita
Published: January 2024

Pulmonary inflammations such as chronic obstructive pulmonary disease and cystic fibrosis are widespread and can be fatal, especially when they are characterized by abnormal mucus accumulation. Inhaled corticosteroids are commonly used for lung inflammations despite their considerable side effects. By utilizing particle engineering techniques, a combined dry powder inhaler (DPI) comprising nanosized ketoprofen-embedded mannitol-coated microparticles was developed. A nanoembedded microparticle system means a novel advance in pulmonary delivery by enhancing local pulmonary deposition while avoiding clearance mechanisms. Ketoprofen, a poorly water-soluble anti-inflammatory drug, was dispersed in the stabilizer solution and then homogenized by ultraturrax. Following this, a ketoprofen-containing nanosuspension was produced by wet-media milling. Furthermore, co-spray drying was conducted with L-leucine (dispersity enhancer) and mannitol (coating and mucuactive agent). Particle size, morphology, dissolution, permeation, viscosity, in vitro and in silico deposition, cytotoxicity, and anti-inflammatory effect were investigated. The particle size of the ketoprofen-containing nanosuspension was ~230 nm. SEM images of the spray-dried powder displayed wrinkled, coated, and nearly spherical particles with a final size of ~2 µm (nano-in-micro), which is optimal for pulmonary delivery. The mannitol-containing samples decreased the viscosity of 10% mucin solution. The results of the mass median aerodynamic diameter (2.4–4.5 µm), fine particle fraction (56–71%), permeation (five-fold enhancement), and dissolution (80% release in 5 min) confirmed that the system is ideal for local inhalation. All samples showed a significant anti-inflammatory effect and decreased IL-6 on the LPS-treated U937 cell line with low cytotoxicity. Hence, developing an innovative combined DPI comprising ketoprofen and mannitol by employing a nano-in-micro approach is a potential treatment for lung inflammations.
Sub-Nanosecond, High Peak Power Yb:YAG/Cr4+:YAG/YVO4 Passively Q-Switched Raman Microchip Laser with the Emission of Multiple Pulses
Authors: Wang Xiaolei , Zhang Chaoyi , Zhang Yanlu , Fan Shengying , Ma Xinqiang , Cheng Wei
Published: January 2024

This paper demonstrates the capability of sub-nanosecond, high peak power Yb:YAG/Cr4+:YAG/YVO4 passively Q-switched Raman microchip lasers at 1134 nm operated in multiple pulses mode under quasi-continuous-wave (QCW) pumping. Total pulse energy for the Stokes laser was 1.8 mJ with a 4 mm YVO4 crystal and TOC = 16%. The corresponding pulse repetition rate reached 225 kHz within a single pumping pulse. By employing a compact plane-concave cavity and 5 mm YVO4 crystal, the single pulse energy for the Raman laser was further scaled up to 44 μJ. The corresponding peak power was 95 kW. A highest output pulse repetition rate of 87.8 kHz and shortest pulse duration of 464 ps were found for the Raman laser. The results indicate that the Raman microchip laser configuration under QCW LD pumping is a promising approach for developing high peak power, commercial and portable Raman lasers with a pulse duration of several hundred-picoseconds at a pulse repetition rate of hundred kilohertz.
Dual-Polarized Reconfigurable Manipulation Based on Flexible-Printed Intelligent Reflection Surface
Authors: Jia Xiaozhe , Tan Hongrui , Dong Xinyu , Ye Fuju , Cui Haoyang , Chen Lei
Published: January 2024

In the background of 6G communication requiring a high data rate and energy efficiency, global coverage and connectivity, as well as high reliability and low latency, most existing reconfigurable metasurfaces face limitations in flexibility, integrability, energy consumption, and cost. This paper proposes a dual-polarized intelligent reflection surface (IRS) based on a paper-based flexible substrate as a solution. The proposed design uniquely enables the independent control of two orthogonally polarized electromagnetic waves to achieve customized scattering effects. Compared to conventional reconfigurable intelligent surfaces using PCB technology and active components, this design utilizes paper as the substrate material combined with conductive ink and silver ink, significantly reducing production costs and process complexity. The manufacturing cost is only about one-tenth of the traditional PCB solutions. This approach is not only cost-effective but also excels in both flexibility and portability. These attributes signify its suitability for a broader range of potential applications, encompassing areas where traditional RIS may be impractical due to cost, rigidity, or complexity constraints. By drawing rotationally symmetric small metal block structures on paper using silver ink, four structures are designed that achieve a phase difference of 90 degrees for both x-polarized and y-polarized wave incidences at the resonant frequency of 4.5754 GHz, realizing independent phase modulation. The dual-polarized flexible 2-bit intelligent reflection surface consists of 20×20 unit cells, and six different coding patterns are designed for single-beam and dual-beam design based on different scattering angles. The experimental results show that this polarization-independent flexible 2-bit intelligent reflection surface structure successfully allows independent control of two orthogonally polarized electromagnetic waves, enabling customized scattering effects. The experimental results are highly consistent with the simulation results. The independent control of two orthogonal polarized electromagnetic waves is a key feature of our design, enabling more flexible and effective signal coverage in complex urban environments. This precise control over polarization not only enhances the adaptability of the system but also offers practical solutions for real-world applications, particularly in meeting the growing demands of urban communication. The proposed metasurface based on paper-based flexible substrate is low-cost and highly portable, and the polarization independence provides more degrees of freedom for the metasurface, which is beneficial for more precise and efficient beam control and can be applied in the field of communication, especially 6G communication and IRS wireless communication. In addition, it also has broad application prospects in radar systems and remote sensing applications.
Identification of Genes Responsible for the Synthesis of Glycitein Isoflavones in Soybean Seeds
Authors: Horitani Masaki , Yamada Risa , Taroura Kanami , Maeda Akari , Anai Toyoaki , Watanabe Satoshi
Published: January 2024

Soybean (Glycine max (L.) Merrill) isoflavones are among the most important secondary metabolites, with functional benefits for human health. Soybeans accumulate three aglycone forms of isoflavones: genistein, daidzein, and glycitein. Soybean landrace Kumachi-1 does not accumulate malonylglycitin at all. Gene structure analysis indicated that Glyma.11G108300 (F6H4) of Kumachi-1 has a 3.8-kbp insertion, resulting in a truncated flavonoid 6-hydroxylase (F6H) sequence compared to the wild-type sequence in Fukuyutaka. Mapping experiments using a mutant line (MUT1246) with a phenotype similar to that of Kumachi-1, with a single-nucleotide polymorphism (SNP) in F6H4, revealed co-segregation of this mutation and the absence of glycitein isoflavones. We also identified a mutant line (K01) that exhibited a change in the HPLC retention time of glycitein isoflavones, accumulating glycoside and malonylglycoside forms of 6-hydroxydaidzein. K01 contains an SNP that produces a premature stop codon in Glyma.01G004200 (IOMT3), a novel soybean isoflavone O-methyltransferase (IOMT) gene. We further analyzed transgenic hairy roots of soybeans expressing Glyma.11G108300 (F6H4) and Glyma.01G004200 (IOMT3). Those overexpressing F6H4 accumulated malonylglycoside forms of 6-hydroxydaidzein (M_6HD), and co-expression of F6H4 and IOMT3 increased the level of malonylglycitin but not of M_6HD. These results indicate that F6H4 and IOMT3 are responsible for glycitein biosynthesis in soybean seed hypocotyl.
Fine-Root Distribution and Soil Physicochemical Property Variations in Four Contrasting Urban Land-Use Types in South Korea
Authors: Tran Lan Thi Ngoc , An Ji Young , Carayugan Mark Bryan , Hernandez Jonathan O. , Rahman SK Abidur , Youn Woo Bin , Carvalho Julia Inacio , Jo Min Seon , Han Si Ho , Nguyen Hai-Hoa , Park Byung Bae
Published: January 2024

Urbanization and associated forest conversions have given rise to a continuum of native (forest fragments) and modified (artificial grasslands and perennial ecosystems) land-use types. However, little is known about how these shifts affect soil and fine-root compartments that are critical to a functioning carbon and nutrient circulation system. In this study, soil physicochemical properties, fine-root mass, and vertical distribution patterns were investigated in four representative urban land-use types: grassland (ZJ), perennial agroecosystem (MP), broadleaf deciduous forest patch (QA), and coniferous evergreen forest patch (PD). We quantified the fine-root mass in the upper 30 cm vertical profile (0–30 cm) and at every 5 cm depth across three diameter classes (<2 mm, 2–5 mm, and <5 mm). Soil physicochemical properties, except for phosphorus, nitrogen, ammonium nitrogen, and sodium cations, varied significantly across land-use types. The total root biomass (<5 mm) decreased in the order of QA (700.3 g m−2) > PD (487.2 g m−2) > ZJ (440.1 g m−2) > MP (98.3 g m−2). The fine-root mass of ZJ and MP was correlated with soil nutrients, which was attributed to intensive management operations, while the fine-root mass of QA and PD had a significant relationship with soil organic matter due to the high inputs from forest litter. Very fine roots (<2 mm) presented a distinct decremental pattern with depth for all land-use types, except for MP. Very fine roots populated the topmost 5 cm layer in ZJ, QA, and PD at 52.1%, 49.4%, and 39.4%, respectively. Maintaining a woody fine-root system benefits urban landscapes by promoting soil stabilization, improving ground infiltration rates, and increasing carbon sequestration capacity. Our findings underscore the importance of profiling fine-root mass when assessing urban expansion effects on terrestrial ecosystems.