bluuebunny/crossref_metadata_2025 huggingface dataset:
Hypoxia shapes the immune landscape in lung injury promoting inflammation persistence
Authors: Mirchandani Ananda S. , Jenkins Stephen J. , Bain Calum C. , Lawson Hannah , Coelho Patricia , Murphy Fiona , Griffith David , Zhang Ailiang , Sanchez-Garcia Manuel A. , Reyes Leila , Morrison Tyler , Arienti Simone , Sadiku Pranvera , Watts Emily R. , Dickinson Rebecca. S. , Clark Sarah , Ly Tony , Lewis David , Kelly Van , Spanos Christos , Musgrave Kathryn M. , Delaney Liam , Harper Isla , Scott Jonathan , Parkinson Nicholas J. , Rostron Anthony J. , Baillie Kenneth J , Clohisey Sara , Pridans Clare , Campana Lara , Starkey-Lewis Philip , Simpson A John , Dockrell David , Schwarze Jurgen , Hirani Nikhil , Ratcliffe Peter J. , Pugh Christopher W. , Kranc Kamil , Forbes Stuart J. , Whyte Moira K. , Walmsley Sarah R.
Published: March 2022

Acute Respiratory Distress Syndrome (ARDS), an often-fatal complication of pulmonary or systemic inflammation, has no cure. Hypoxemia is a defining feature, yet its impact on inflammation is often neglected. Patients with ARDS are monocytopenic early in the onset of the disease. Endotoxin or Streptococcus pneumoniae acute lung injury (ALI) in the context of hypoxia replicates this finding, through hypoxia-driven suppression of type I interferon signalling. This results in failed lung monocyte-derived interstitial macrophages (IM) niche expansion and unchecked neutrophilic inflammation. Administration of colony stimulating factor 1 (CSF1) rescues the monocytopenia, alters the circulating classical monocyte phenotype in hypoxic endotoxin-driven ALI and enables lung IM population expansion, thus limiting lung injury in endotoxin- and virally-induced hypoxic ALI. Hypoxia directly alters immune dynamics to the detriment of the host and manipulation of this aberrant response offers new therapeutic strategies for ARDS.
The mechanical stability of Tension Gauge Tethers
Authors: Liu Jingzhun , Le Shimin , Yao Mingxi , Huang Wenmao , Tio Zhikai , Zhou Yu , Yan Jie
Published: March 2022

Mechanotransduction of cells relies on responding to tension transmitted along various supramolecular linkages. Tension gauge tethers (TGTs), short double-stranded DNA (dsDNA) fragments that undergo irreversible tension-dependent dissociation under shear-stretching mode, have been widely applied in live cell experiments to provide critical insights into the mechanotransduction activities of cells. However, the current physical understanding of the mechanical responses of TGTs remains limited, which restricts the range of information that can be extracted from experimental observations. In order to provide quantitative in-depth understanding and interpretation of experimental observations, in this work, we quantified the tension-dependent lifetime of TGTs from which the mechanical stability of TGTs under various physiologically relevant stretching conditions can be derived. Applications of the determined mechanical stability of TGTs to cell studies strongly suggest revisiting the previous interpretations of several reported experimental observations.
Kaposi’s sarcoma-associated herpesvirus induces specialised ribosomes to efficiently translate viral lytic mRNAs
Authors: Murphy James C. , Schumann Sophie , Harrington Elena , Vasconcelos Elton J. R. , Mottram Timothy J. , Aspden Julie L. , Whitehouse Adrian
Published: March 2022

Historically, ribosomes have been viewed as unchanged homogeneous macromolecular machines with no intrinsic regulatory capacity for mRNA translation. However, an emerging concept is that heterogeneity of ribosomal composition exists, which can exert a regulatory function or specificity in translational control. This is supported by recent discoveries identifying compositionally distinct ‘specialised ribosomes’ that actively regulate mRNA translation. Viruses lack their own translational machinery and impose a high translational demand on the host cell during replication. Here we explore the possibility that Kaposi’s sarcoma-associated herpesvirus (KSHV) can manipulate host ribosome biogenesis during infection to produce specialised ribosomes which preferentially translate viral transcripts. Quantitative proteomic analysis has identified changes in the stoichiometry and composition of precursor ribosomal complexes during the switch from latent to lytic KSHV replication. Intriguingly, we demonstrate the enhanced association of ribosomal biogenesis factors BUD23 and NOC4L, and a previously uncharacterised KSHV lytic protein, ORF11, with small ribosomal subunit precursor complexes during lytic KSHV infection. Notably, BUD23 depletion resulted in significantly reduced viral gene expression and progression through the lytic cascade, culminating in a dramatic reduction of infectious virion production. Importantly, ribosome profiling demonstrated that BUD23 is essential for the reduced association of ribosomes with KSHV uORFs in late lytic genes, required for the efficient translation of the main open reading frame. Together our results provide new mechanistic insights into KSHV-mediated manipulation of cellular ribosome composition inducing a population of specialised ribosomes to facilitate efficient translation of viral mRNAs.
The KASH5 protein involved in meiotic chromosomal movements is a novel dynein activating adaptor
Authors: Agrawal Ritvija , Gillies John P. , Zang Juliana L. , Zhang Jingjing , Garrott Sharon R. , Shibuya Hiroki , Nandakumar Jayakrishnan , DeSantis Morgan E.
Published: March 2022

Dynein harnesses ATP hydrolysis to move cargo on microtubules in multiple biological contexts. Dynein meets a unique challenge in meiosis by moving chromosomes tethered to the nuclear envelope to facilitate homolog pairing essential for gametogenesis. Though processive dynein motility requires binding to an activating adaptor, the identity of the activating adaptor required for dynein to move meiotic chromosomes is unknown. We show that the meiosis-specific nuclear-envelope protein KASH5 is a dynein activating adaptor: KASH5 directly binds dynein using a mechanism conserved among activating adaptors and converts dynein into a processive motor. We map the dynein-binding surface of KASH5, identifying mutations that abrogate dynein binding in vitro and disrupt recruitment of the dynein machinery to the nuclear envelope in cultured cells and mouse spermatocytes in vivo. Our study identifies KASH5 as the first transmembrane dynein activating adaptor and provides molecular insights into how it activates dynein during meiosis.
Alveolar regeneration following viral infection is independent of tuft cells
Authors: Huang Huachao , Jiang Ming , Zhang Yihan , Biermann Jana , Melms Johannes C. , Danielsson Jennifer A. , Fang Yinshan , Yang Ying , Qiang Li , Liu Jia , Zhou Yiwu , Wang Manli , Hu Zhihong , Wang Timothy C. , Saqi Anjali , Sun Jie , Matsumoto Ichiro , Cardoso Wellington , Emala Charles W. , Zhu Jian , Izar Benjamin , Mou Hongmei , Que Jianwen
Published: March 2022

Severe injuries following viral infection cause lung epithelial destruction with the presence of ectopic basal progenitor cells (EBCs), although the exact function of EBCs remains controversial. We and others previously showed the presence of ectopic tuft cells in the disrupted alveolar region following severe influenza infection. Here, we further revealed that the ectopic tuft cells are derived from EBCs. This process is amplified by Wnt signaling inhibition but suppressed by Notch inhibition. Further analysis revealed that p63-CreER labeled population de novo arising during regeneration includes alveolar epithelial cells when Tamoxifen was administrated after viral infection. The generation of the p63-CreER labeled alveolar cells is independent of tuft cells, demonstrating segregated differentiation paths of EBCs in lung repair. EBCs and ectopic tuft cells can also be found in the lung parenchyma post SARS-CoV-2 infection, suggesting a similar response to severe injuries in humans.
PplT domain proteins – ubiquitous potential prokaryotic phospholipid translocases
Authors: Hauser Janna N. , Kengmo Tchoupa Arnaud , Zabel Susanne , Nieselt Kay , Ernst Christoph M. , Slavetinsky Christoph J. , Peschel Andreas
Published: March 2022

Flippase proteins exchanging phospholipids between the cytoplasmic membrane leaflets have been identified in Eukaryotes but remained largely unknown in Prokaryotes. Only MprF proteins that synthesize aminoacyl phospholipids in some bacteria have been found to contain a domain that translocates the produced lipids. We show here that this domain, which we named ‘prokaryotic phospholipid translocator’ (PplT), is widespread in Bacteria and Archaea, encoded as a separate protein or fused to different types of enzymes. We also demonstrate that theEscherichia coliPplT protein interacts with many phospholipid-synthetic enzymes and deletion ofpplTimpaired bacterial growth, which supports its potential role in membrane lipid metabolism. PplT domain proteins may be general prokaryotic lipid flippases with critical roles in cellular homeostasis.
IFN-induced protein with tetratricopeptide repeats 2 (Ifit2) limits autoimmune inflammation by regulating myeloid cell activation and metabolic activity
Authors: Kim Dongkyun , Rai Nagendra Kumar , Burrows Amy , Kim Sohee , Tripathi Ajai , Weinberg Samuel E. , Dutta Ranjan , Sen Ganes C. , Min Booki
Published: March 2022

Besides anti-viral functions, Type I IFN expresses potent anti-inflammatory properties and is being widely used to treat certain autoimmune conditions, such as multiple sclerosis (MS). In murine model of MS, experimental autoimmune encephalomyelitis (EAE), administration of IFNβ effectively attenuates the disease development. However, the precise mechanisms underlying the treatment remain elusive. In this study, we report that IFN-induced protein with tetratricopeptide repeats 2 (Ifit2), a type I and type III IFN-stimulated gene, plays a previously unrecognized immune regulatory role during autoimmune neuroinflammation. Mice deficient in Ifit2 display greater susceptibility to EAE and escalated immune cell infiltration in the central nervous system. Ifit2 deficiency is also associated with microglial activation and increased myeloid cell infiltration. Unexpectedly, myelin debris clearance and the subsequent remyelination is impaired in Ifit2-/- CNS tissues. Clearing myelin debris is an important property of reparative M2 type myeloid cells to promote remyelination. Indeed, we observed that bone marrow derived macrophages, CNS infiltrating myeloid cells, and microglia from Ifit2-/- mice express cytokine and metabolic genes associated with proinflammatory M1 type subsets. Taken together, our findings uncover a novel regulatory function of Ifit2 in autoimmune inflammation in part by modulating myeloid cell function and metabolic activity.
The role ofUBE3Ain the autism and epilepsy-related Dup15q syndrome using patient-derived, CRISPR-corrected neurons
Authors: Elamin Marwa , Dumarchey Aurelie , Stoddard Christopher , Robinson Tiwanna M. , Cowie Christopher , Gorka Dea , Chamberlain Stormy J. , Levine Eric S.
Published: March 2022

Chromosome 15q11-q13 duplication syndrome (Dup15q) is a neurodevelopmental disorder caused by maternal duplications of this region. Autism and epilepsy are key features of Dup15q, but affected individuals also exhibit intellectual disability and developmental delay.UBE3A, the gene encoding the ubiquitin protein ligase E3A, is likely a major driver of Dup15q because individuals with maternal, but not paternal 15q duplications have the disorder, andUBE3Ais the only imprinted gene expressed solely from the maternal allele in mature neurons. Nevertheless, the exact role ofUBE3Ais yet to be determined. To establish whetherUBE3Aoverexpression is required for Dup15q neuronal deficits,UBE3Aexpression was manipulated in patient-derived induced pluripotent stem cell (iPSC) lines using antisense oligonucleotides. Dup15q neurons exhibited hyperexcitability features compared to genome-edited isogenic control neurons, and this phenotype was generally prevented by normalizingUBE3Alevels throughoutin vitrodevelopment. Overexpression ofUBE3Ain an iPSC line with a paternal duplication resulted in a profile similar to that of Dup15q neurons except for synaptic phenotypes. These results indicate thatUBE3Aoverexpression is necessary for most Dup15q cellular phenotypes. However, the inability ofUBE3Aoverexpression to recapitulate synaptic phenotypes suggests an important role for non-imprinted genes in the disorder.
Neuromodulation of striatal D1 cells shapes BOLD fluctuations in anatomically connected thalamic and cortical regions
Authors: Markicevic Marija , Sturman Oliver , Bohacek Johannes , Rudin Markus , Zerbi Valerio , Fulcher Ben D. , Wenderoth Nicole
Published: March 2022

Understanding how the brain’s macroscale dynamics are shaped by underlying microscale mechanisms is a key problem in neuroscience. In animal models, we can now investigate this relationship in unprecedented detail by directly manipulating cellular-level properties while measuring the whole-brain response using resting-state fMRI. Here we focused on understanding how blood-oxygen-level-dependent (BOLD) dynamics, measured within a structurally well-defined striato-thalamo-cortical circuit, are shaped by chemogenetically exciting or inhibiting D1 medium spiny neurons (MSNs) of the right dorsomedial striatum (CPdm). We characterize changes in both the BOLD dynamics of individual cortical and subcortical brain areas, and patterns of inter-regional coupling (functional connectivity) between pairs of areas. Using a classification approach based on a large and diverse set of time-series properties, we found that CPdm neuromodulation alters BOLD dynamics within thalamic subregions that project back to dorsomedial striatum. In the cortex, the strongest changes in local dynamics were observed in unimodal regions, i.e., regions that process information from a single sensory modality, while changes in the local dynamics weakened along a putative cortical hierarchical gradient towards transmodal regions. In contrast, a decrease in functional connectivity was observed only for cortico-striatal connections after D1 excitation. Our results provide a comprehensive understanding of how targeted cellular-level manipulations affect local BOLD dynamics at the macroscale, including the role of a circuit’s structural characteristics and hierarchical cortical level in shaping those dynamics. These findings contribute to ongoing attempts to understand the influence of structure–function relationships in shaping inter-regional communication at subcortical and cortical levels.
The role ofStaphylococcus agnetisandStaphylococcus hyicusin the pathogenesis of buffalo fly skin lesions in cattle
Authors: Naseem Muhammad Noman , Turni Conny , Gilbert Rosalind , Raza Ali , Allavena Rachel , McGowan Michael , Constantinoiu Constantin , Ong Chian Teng , Tabor Ala E. , James Peter
Published: March 2022

Buffalo flies (Haematobia irritans exigua) are hematophagous ectoparasites of cattle causing production and welfare impacts in northern Australian herds. Skin lesions associated with buffalo fly infestation andStephanofilarianematode infection are manifested as focal dermatitis or ulcerated areas most commonly on the medial canthus of the eye, along the lateral and ventral neck and on the abdomen of cattle. For closely related horn flies (Haematobia irritans irritans),Staphylococcus aureushave been suggested as a contributing factor in the development of lesions. To investigate the potential role of bacterial infection in the pathogenesis of buffalo fly lesions, swabs were taken from lesions and normal skin, and bacteria were also isolated from surface washings of buffalo flies and surface-sterilised homogenized flies. Bacterial identification was conducted by MALDI-TOF, strain typing by rep-PCR and DNA sequencing to determine species similarity and virulence factors. Of 49 bacterial isolates collected from lesions, 37 were identified asStaphylococcus agnetisand 12 asStaphylococcus hyicus, whereas from normal skin four isolates wereS. hyicusand one wasStaphylococcus sciuri. Of theStaphylococcusisolates isolated from buffalo flies, five were identified asS. agnetisand three asS. hyicus. Fifty percent of the buffalo fly isolates had rep-PCR genotypic patterns identical to the lesion isolates. Genome sequencing of 16S. agnetisand fourS. hyicusisolates revealed closely similar virulence factor profiles, with all isolates possessing exfoliative toxin A and C genes. The findings from this study suggest the involvement ofS. agnetisandS. hyicusin buffalo fly lesion pathogenesis. This should be taken into account in the development of effective treatment and control strategies for lesions.
Cryo-EM structure of amyloid fibril formed by α-synuclein hereditary A53E mutation
Authors: Sun Chuanqi , Zhou Kang , DePaola Peter , Shin Woo Shik , Hillyer Trae , Sawaya Michael R. , Zhou Z. Hong , Jiang Lin
Published: March 2022

Synucleinopathies, including Parkinson’s disease (PD), dementia with Lewy bodies (DLB), and multiple systems atrophy (MSA) have the same hallmark pathologic feature of misfolded α-synuclein protein accumulation in the brain. PD patients who carry α-syn hereditary mutations tend to have an earlier onset and more severe clinical symptoms and pathology than sporadic PD patients who carry wild-type (WT) α-syn. Therefore, revealing the structural effect of α-syn hereditary mutations on the wild-type fibril structure can help us understand synucleinopathies’ structural basis. Here, we present a 3.38 Å cryo-electron microscopy structure of α-synuclein fibrils containing the hereditary A53E mutation. The A53E fibril is symmetrically composed of two protofilaments, as are many other synucleopathic structures – including WT. Interestingly, the interface between the protofilaments in A53E has significantly less buried surface area than all other documented fibril structures of α-syn and its other mutants. The A53E fibril also exhibits slower formation/growth in in vitro fibrillation experiment compared to other mutants. This implies that the structural differences - both in the protofilament and between each protofilament of A53E – change the aggregation mechanism, or in the least, its kinetics of formation. These differences influence the molecular characteristics of each fibril mutant and likely plays a macro-scale role in progressing one clinical pathology over another.
Exploring the link between learning from error climate and professionals' engagement in social learning activities after errors
Authors: Smeets Laura , Gijselaers Wim , Meuwissen Roger , Grohnert Therese
Published: March 2022

Purpose

Learning from errors is a complex process that requires careful support. Building on affective events theory, the purpose of this paper is to explore how a supportive learning from error climate can contribute to social learning from errors through affective and cognitive error responses by individual professionals.

Design/methodology/approach

A total of 139 early-career auditors completed an online questionnaire consisting of validated survey scales, allowing for serial mediation analysis to compare direct and indirect effects.

Findings

Learning from error climate was directly and positively related to engagement in social learning activities after committing an error. Furthermore, the authors found a double mediation by error strain (an affective error response) and reflecting on errors (a cognitive error response) on this relationship.

Practical implications

Organizations can actively encourage professionals to learn from their errors by creating a supportive learning from error climate and holding professionals accountable for their errors.

Originality/value

The present study enriches the authors’ understanding of the mechanisms through which learning from error climate influences engagement in social learning activities. It extends prior research on learning from errors by investigating the sequential effects of engagement in error-related learning activities performed individually and in social interaction.
Understanding open data business models from innovation and knowledge management perspectives
Authors: Corrales-Garay Diego , Ortiz-de-Urbina-Criado Marta , Mora-Valentín Eva-María
Published: March 2022

Purpose

This paper aims to analyse the open data business models (ODBMs) as a source of knowledge and innovation to generate economic and social value. A framework for understanding ODBMs is presented. First, the knowledge structure of the ODBMs literature is identified. Second, a conceptual model for analysing the ODBMs is proposed. And finally, the future trends in ODBMs research are discussed.

Design/methodology/approach

In this paper, co-word analysis is performed to identify the topics related with ODBMs.

Findings

The ODBMs structure of knowledge is articulated in five themes: business model, smart city, business ecosystem, decision-making and innovation. Based on these results, a five-step model for analysing ODBMs is proposed. Finally, a discussion of the future trends of ODBMs focussed on a knowledge management perspective, open data ecosystems and business intelligence is presented.

Originality/value

The paper presents a picture of what, where, for whom and for what ODBMs have been studied and develops a new model to explain the value creation process of ODBMs. Taking a step further, applying the principles and models of knowledge management and business intelligence to ODBMs is also recommended in order to transfer and transform open data into valuable knowledge that can be used for developing apps. In that context, the importance of encouraging collaboration between different agents in the so-called open data ecosystem is presented.
Overeducation wage penalty among Ph.D. holders: an unconditional quantile regression analysis on Italian data
Authors: Gaeta Giuseppe Lucio , Lubrano Lavadera Giuseppe , Pastore Francesco
Published: March 2022

Purpose

The wage effect of job–education vertical mismatch (i.e. overeducation) has only recently been investigated in the case of Ph.D. holders. The existing contributions rely on ordinary least squares (OLS) estimates that allow measuring the average effect of being mismatched at the mean of the conditional wage distribution.

Design/methodology/approach

The authors implement a recentered influence function (RIF) to estimate the overeducation gap along the entire hourly wage distribution and compare Ph.D. holders who are overeducated with those who are not on a specific sample of Ph.D. holders in different fields of study and European Research Council (ERC) categories. Moreover, the authors compare the overeducation gap between graduates working in the academic and non-academic sector.

Findings

The results reveal that overeducation hits the wages of those Ph.D. holders who are employed in the academic sector and in non-research and development (R&D) jobs outside of the academic sector, while no penalty exists among those who carry out R&D activities outside the academia. The size of the penalty is higher among those who are in the mid-top of the wage distribution and hold a Social Science and Humanities specialization.

Practical implications

Two policies could reduce the probability of overeducation: (a) a reallocation of Ph.D. grants from low to high demand fields of study and (b) the diffusion of industrial over academic Ph.Ds.

Originality/value

This paper observes the heterogeneity of the overeducation penalty along the wage distribution and according to Ph.D. holders' study field and sector of employment (academic/non-academic).
Investigating boycotts and buycotts as antecedents towards attitude and intention to engage in ethical consumption
Authors: Akintimehin Olamide , Phau Ian , Ogbechie Rose , Oniku Ayodele
Published: March 2022

Purpose

This paper aims to investigate the attitude towards ethical consumption and intention to engage in ethical consumption behaviour by consumers within a developing economy, building on the ethically minded consumer behaviour (EMCB) framework.

Design/methodology/approach

A quantitative research approach was adopted in this research. A cross-sectional survey was further done in collecting data from 397 respondents through an online questionnaire. Collected data was analysed using the Statistical Package for Social Sciences version 25, as well as the structural equation model via the Analysis of Moment Structures version 23.

Findings

Corporate social responsibility-mindedness was found to lead to a positive attitude towards ethical consumption behaviour and an intention to engage in ethical consumption behaviour; recycling-mindedness did not influence a positive attitude towards ethical consumption behaviour but influenced an intention to engage in ethical consumption behaviour. However, eco-mindedness did not influence a positive attitude towards ethical consumption behaviour and an intention to engage in ethical consumption behaviour: findings from this research showed that a positive attitude towards ethical consumption behaviour led to an intention to engage in ethical consumption behaviour.

Originality/value

To the best of the authors’ knowledge, this research is the first within a Sub-Saharan African region of a developing economy to adapt the EMCB framework in investigating the extent to which consumers within a developing economy intend to engage in ethical consumption behaviour.
Individuals' fixed digital mindset, internal HRM alignment and feelings of helplessness in virtual teams
Authors: Wong Sut I , Solberg Elizabeth , Traavik Laura
Published: March 2022

Purpose

The present study investigates whether individuals having a fixed digital mindset (comprises fundamental beliefs about technological ability and organizational resources as work becomes more digitalized) experience greater helplessness working in virtual teamwork environments. The authors examine how perceived internal human resource management (HRM) alignment moderates the positive relationship expected between individuals' fixed digital mindset and feelings of helplessness. Together, the paper aims to contribute to a greater understanding of the personal and contextual factors that influence an individual's experience of helplessness in virtual team settings.

Design/methodology/approach

The authors test the hypotheses using time-lagged survey data collected from 153 information technology (IT) engineers working in virtual teams in Europe.

Findings

The authors find that individuals with higher levels of fixed digital mindset experience greater helplessness in virtual teamwork environments than individuals with lower levels. Furthermore, the authors find that having higher-fixed beliefs about organizational resources is positively related to helplessness when individuals perceive that the broader HRM system is misaligned with the virtual teamwork environment.

Research limitations/implications

The data were obtained from IT engineers in Europe, which is potentially limiting the generalizability of the authors' findings to other work contexts and cultures.

Practical implications

The authors' study helps leaders in virtual teamwork environments to better understand and manage the personal and contextual factors that could affect individuals' well-being and effective functioning in such settings.

Originality/value

The authors' research contributes to the scant literature investigating the personal characteristics important in virtual teamwork environments and the contextual factors important for aligning virtual teamwork designs with the organizational system. The authors extend this research by looking at personal and contextual factors together in a single model.
Shaping path of trust: the role of information credibility, social support, information sharing and perceived privacy risk in social commerce
Authors: Tseng Hsiao-Ting
Published: March 2022

Purpose

Currently, the Covid-19 pandemic is changing how consumers shop, encouraging deeper levels and increased reliance on e-commerce systems and social media such as online communities. The online interaction of consumers is more important and brings many uncertainties, which impact the original commerce environment. This study aim to investigate the ethical consideration of information credibility and perceived privacy risk from a psychology perspective in marketing, this study draws on social support theory from social psychology to develop a research model to investigate the role of information credibility and perceived privacy risk on social commerce websites.

Design/methodology/approach

This study investigated social commerce websites and communities. The research data of this study was collected through a questionnaire from consumers on three famous social commerce platforms. Using PLS-SEM to perform data analysis, this study research the importance of information credibility, perceived privacy risk and trust on social commerce websites.

Findings

The findings discuss individuals' reaction to privacy issues and to understand the motives to disclose or reveal personal information within a marketing or consumption context. The research also explores the theoretical implications by integrating theories from information systems and social psychology to investigate ethical issues in social commerce.

Originality/value

Covid-19 makes peer-to-peer communication in online communities is developing collaborative consumption, and information produced in these communities can influence the decisions of consumers. Covid-19 has exacerbated such a change in social commerce environment. Therefore, information credibility plays an important role in developing online communities. It is important to look at the psychological antecedents that drive consumers' willingness to share their personal information when using online communities. The author has clarified which aspects of trust in social commerce should be strategized, including information trustworthiness, perceived privacy risks, social support and information sharing. These are the details that companies should pay more attention to when operating social commerce. Only by paying more attention to these details and giving consumers a positive feeling can consumers' trust be maintained or enhanced, ultimately leading to a successful trust economy.
The intricate relationships of consumers’ loyalty and their perceptions of service quality, price and satisfaction in restaurant service
Authors: Ahmed Selim , Al Asheq Ahmed , Ahmed Ezaz , Chowdhury Ujjal Yaman , Sufi Tahir , Mostofa Md. Golam
Published: March 2022

Purpose

The purpose of this study is to determine how consumers perceive restaurant service in Bangladesh. The goal of this study is to determine the impact of perceived service quality and price fairness on consumer satisfaction and loyalty toward restaurant services.

Design/methodology/approach

The data for this study were collected from 326 respondents who have used restaurant services in Bangladesh. Partial Least Squares Structural Equation Modeling (PLS-SEM) was used to evaluate the research data with SmartPLS 3.3.3.

Findings

The current study’s findings indicate that perceived service quality and price fairness both have a direct and significant effect on consumer satisfaction. Additionally, the research findings reveal a considerable association between perceived pricing fairness and consumer pleasure and consumer loyalty. Additionally, findings indicate that perceived service quality and price fairness both have a significant indirect effect on customer loyalty via the mediating effect of consumer satisfaction.

Practical implications

The current study demonstrates that perceived price fairness has a substantial effect on restaurant consumers’ satisfaction and loyalty; thus, this evidence can assist restaurant owners and managers in developing and implementing their pricing strategy to retain customers. Additionally, the findings have significant implications for restaurant operators, practitioners and policymakers.

Originality/value

Only a few research have been conducted to determine the effect of service quality and pricing fairness on consumer satisfaction and loyalty when it comes to restaurant services. This research conclusion provides guidance to service providers on how to increase customer satisfaction and loyalty through an emphasis on price fairness and service quality.
Ecological engineering across a spatial gradient: Sociable weaver colonies facilitate animal associations with increasing environmental harshness
Authors: Lowney Anthony M. , Thomson Robert L.
Published: March 2022

The spatial distribution of animals in a landscape depends mainly on the distribution of resources. Resource availability is often facilitated by other species and can positively influence local species diversity and affect community structure. Species that significantly change resource availability are often termed ecosystem engineers. Identifying these species is important, but predicting where they have large or small impacts is a key challenge that will enhance the usefulness of the ecosystem engineering concept.

In harsh and stressful environments, the stress gradient hypothesis predicts that community structure and function will be increasingly influenced by facilitative interactions.

To test this hypothesis, we investigate how the ecosystem engineering role and importance of sociable weavers Philetairus socius varies across a spatial gradient of harshness, for which aridity served as a proxy. These birds build large colonies that are home to hundreds of weavers and host a wide range of avian and non‐avian heterospecifics. We investigated the use of weaver colonies on multiple taxa (invertebrates, reptiles, birds and mammals) at multiple sites across a >1,000 km aridity gradient.

We show that sociable weaver colonies create localized biodiversity hotspots across their range. Furthermore, trees containing sociable weaver colonies maintained localized animal diversity at sites with lower rainfall, an effect not as pronounced at sites with higher rainfall.

Our results were consistent with predictions of the stress gradient hypothesis, and we provide one of the first tests of this hypothesis in terrestrial animal communities. Facilitation and amelioration by ecosystem engineers may mitigate some of the extreme impacts of environmental harshness.
Citri Reticulatae Pericarpium alleviates postmyocardial infarction heart failure by upregulating PPARγ expression
Authors: Chen Mengli , Zhu Hongyan , Zhu Qingqing , Wu Xiaodong , Zhou Yufei , Gao Rongrong , Shi Mengsha , Zhang Ting , Yin Ting , Zhang Haifeng , Shang Hongcai , Li Xinli
Published: March 2022

Heart failure after myocardial infarction (MI) is the leading cause of death worldwide. Citri Reticulatae Pericarpium (CRP) is a traditional Chinese herbal medicine that has been used in the clinic for centuries. In this study, we aimed to investigate the roles of CRP in cardiac remodelling and heart failure after MI, as well as the molecular mechanisms involved. Male C57BL/6 mice aged 8 weeks were subjected to coronary artery ligation to mimic the clinical situation in vivo. Echocardiography was used to assess the systolic function of the mouse heart. Masson trichrome staining and Wheat germ agglutinin (WGA) staining were utilised to determine the fibrotic area and cross‐sectional area of the mouse heart, respectively. Cardiomyocytes and fibroblasts were isolated from neonatal rats aged 0–3 days in vitro using enzyme digestion. TUNEL staining and EdU staining were performed to evaluate apoptosis and proliferation, respectively. Gene expression changes were analysed by qRT–PCR, and protein expression changes were assessed by Western blotting. Our findings revealed that CRP attenuated cardiac hypertrophy, fibrosis and apoptosis and alleviated heart failure after MI in vivo. Furthermore, CRP mitigated cardiomyocyte apoptosis and fibroblast proliferation and differentiation into myofibroblasts. In addition, the PPARγ inhibitor T0070907 completely abolished the abovementioned beneficial effects of CRP, and the PPARγ activator rosiglitazone failed to further ameliorate cardiac apoptosis and fibrosis in vitro. CRP alleviates cardiac hypertrophy, fibrosis, and apoptosis and can ameliorate heart failure after MI via activation of PPARγ.
Energy‐conserving dimethyl sulfoxide reduction in the acetogenic bacterium Moorella thermoacetica
Authors: Rosenbaum Florian P. , Poehlein Anja , Daniel Rolf , Müller Volker
Published: March 2022

Moorella thermoacetica is one of the well‐studied thermophilic acetogenic bacteria. It grows by oxidation of organic substrates, CO or H2 coupled to CO2 reduction to acetate. Here, we describe that M. thermoacetica can also use dimethyl sulfoxide as terminal electron acceptor. Growth of M. thermoacetica on glucose or H2 + CO2 was stimulated by dimethyl sulfoxide (DMSO). Membranes showed a DMSO reductase activity, that was induced by growing cells in presence of DMSO. The enzyme used reduced anthraquinone‐2,6‐disulfonate, benzyl‐ and methyl viologen as electron donor, but not NAD(P)H. Activity was highest at pH 5 and 60°C, the Km for DMSO was 2.4 mM. Potential DMSO reductase subunits were identified by peptide mass fingerprinting; they are encoded in a genomic region that contains three potential dmsA genes, three dmsB genes and one dmsC gene. Transcriptome analysis revealed that two different dmsAB gene clusters were induced in the presence of DMSO. The function of these two and their predicted biochemical features are discussed. In addition, the data are in line with the hypothesis that M. thermoacetica can use DMSO alongside CO2 as electron acceptor and DMSO reduction is catalysed by an energy‐conserving, membrane‐bound electron transport chain with DMSO as final electron acceptor.
Damned if you do, damned if you don't: How formerly incarcerated men navigate the labor market with prison credentials*
Authors: Lindsay Sadé L.
Published: March 2022

Although employment is central to successful reentry, formerly incarcerated people struggle to find work because of criminal stigma, poor education, and sparse work histories. Prison credentials are proposed as one solution to alleviate these challenges by signaling criminal desistance and employability. Evidence regarding their efficacy, however, is inconsistent. In this article, I develop a novel explanation—the prison credential dilemma—highlighting the numerous and contradictory ways employers may interpret prison credentials as positive and negative signals. Drawing on 50 qualitative interviews with formerly incarcerated men in Franklin County, Ohio, I examine how the prison credential dilemma and the uncertainty it produces shape their job search strategies and pathways to employment. I find that participants concealed or obscured institutional affiliations of prison credentials on job applications to signal employability rather than their criminal records. In job interviews, however, prison credentials were used to divert conversations away from their criminal record toward skills and criminal desistance via the use of redemptive narratives. Participants also attempted to acquire credentials outside of prison and/or pursued temporary, precarious jobs, aspiring for such physically strenuous and poorly paid work to materialize into stable employment. This study has implications for prison programming as well as policies and practices aiming to improve reentry outcomes.
Enhancing learning in small businesses
Authors: Coetzer Alan , Redmond Janice , Sharafizad Jalleh , Lundy Judy
Published: March 2022

This study addresses knowledge gaps relating to how small business owner/managers facilitate employee learning and how distinctive characteristics of small businesses affect employee learning. Data were collected through interviews with owner/managers and employees and observation of conditions in the physical, social, and organisational environments that might help or hinder informal learning. The findings provide a situated, context‐sensitive, and triangulated account relating to two research questions. We find that owner/manager physical and social proximity to employees is a key facilitating factor. Proximity creates the potential for owner/managers to facilitate learning through modelling, provision of immediate feedback and guidance. The owner/manager's positive influence on learning stemming from their physical and social proximity to employees is accentuated by owner/managers employing management styles facilitative of learning. Our findings suggest that a “family‐like” culture and the social cohesion in the businesses had enabling effects on employee participants' informal learning, while their lack of opportunities for formal learning would have inhibiting effects.
Genesis of the Taishanmiao Au Deposit in the Ningshan–Zhenan Ore Field, South Qinling Orogen, China: Evidence from Geochemical, Geochronological and S‐Pb‐H‐O Isotopical Study
Authors: GAO Zuoyu , LIU Yunhua , SUN Naiquan , LI Yuntao , LI Bo
Published: March 2022

The Taishanmiao Au deposit is in the western part of the Ningshan–Zhenan ore field, in the South Qinling orogen. Based on geological and geochemical features, we propose that the Taishanmiao Au deposit is a magmatic‐hydrothermal type of deposit. All samples have high SiO2, K2O + Na2O contents and differentiation index values, low CaO, MgO, P2O5, and TiO2contents, are enriched in high field‐strength elements, and depleted in large ion lithophile element. The stable isotopeδ34S values of pyrite vary from 6.8%–7.8%, and the H‐O isotopic compositions of quartz from quartz‐pyrite veins indicate the ore‐forming fluid is a mixture of a small amount of magmatic‐hydrothermal solution and groundwater. Lead isotope ratios of pyrite and silicalite can show that the ore‐forming materials were derived from a mixed source containing mantle and crustal materials. At the same time, the LA‐ICP‐MS U‐Pb dating of monzogranite is 198.4 ± 4.2 Ma. Combined with the regional geological background, the intracontinental extension in the late collisional orogeny and large‐scale lithospheric thinning associated with mantle uplift may lead to large‐scale mineralisation in the region.
Pore‐Fracture Distribution Heterogeneity of Shale Reservoirs Determined by using HPMI and LPN2 GA Tests
Authors: ZHANG Junjian , QIN Zhengyuan , HAN Yanning , WANG Huaimeng , HOU Maoguo , YAN Gaoyuan , FENG Guangjun , ZHANG Xiaoyang , YIN Tingting , ZHANG Hainan , WEN Shupeng
Published: March 2022

The compressibility of shale matrix reflects the effects of reservoir lithology, material composition, pore structure and tectonic deformation. It is important to understand the factors that influence shale matrix compressibility (SMC) and their effects on pore size distribution (PSD) heterogeneity in order to evaluate the properties of unconventional reservoirs. In this study, the volumes of pores whose diameters were in the range 6–100 nm were corrected for SMC for 17 shale samples from basins in China using high‐pressure mercury intrusion and low‐temperature nitrogen gas adsorption analyses, in order to investigate the factors influencing the SMC values. In addition, the variations in fractal dimensions before and after pore volume correction were determined, using single and multifractal models to explain the effects of SMC on PSD heterogeneity. In this process, the applicability of each fractal model for characterizing PSD heterogeneity was determined using statistical analyses. The Menger and Sierpinski single fractal models, the thermodynamic fractal model and a multi‐fractal model were all used in this study. The results showed the following. The matrix compression restricts the segmentation of the fractal dimension curves for the single fractal Menger and Sierpinski models, which leads to a uniformity of PSD heterogeneity for different pore diameters. However, matrix compression has only a weak influence on the results calculated using a thermodynamic model. The SMC clearly affects the multifractal value variations, showing that the fractal dimension values of shale samples under matrix compression are small. Overall PSD heterogeneity becomes small for pores with diameters below 100 nm and the SMC primarily affects the PSD heterogeneity of higher pore volume areas. The comparison of fractal curves before and after correction and the variance analysis indicate that the thermodynamic model is applicable to quantitatively characterize PSD heterogeneity of shale collected from this sampling area. The results show that PSD heterogeneity increases gradually as micro‐pore volumes increase.
Thalassinoides Ichnofabrics of Lower Cambrian Longwangmiao Formation (Stage 4, Toyonian) on the Yangtze Platform, South China: Improving Paleoenvironmental Interpretations
Authors: TANG Ruifeng , ZHANG Jizhi , DU Yao , WEN Siying , ZHONG Bo , JIANG Huachuan
Published: March 2022

An analysis of Thalassinoides ichnofabrics in the Cambrian Stage 4 Longwangmiao Formation from six sections near Chonqing in the Middle and Upper Yangtze Block of South China was conducted to promote the understanding of the paleoenvironment of this period. Thalassinoides ichnofabrics are divided into three types according to their morphology, bioturbation index, abundance and related parameters: banded, mottled and grid. The completeness of the ichnofabrics gradually increases from banded to grid, the scale of the burrows expands, and the structure transitions from two‐dimensional to three‐dimensional. Malacostracans, phyllocarids and enteropneusts are the most likely burrowers. The findings reveal that frequent changes in paleoenvironmental conditions are the dominant factors for generating the various Thalassinoides ichnofabrics. The changes in depositional rate, substrate properties and nutrient levels affect the behavior and building patterns of the burrowers, thus leading to the diversity of Thalassinoides ichnofabrics. In the lower Longwangmiao Formation beds, many incomplete Thalassinoides burrow systems were built in the substrates with terrigenous detrital minerals, revealing significant environmental pressure on the burrowers. Later, the Thalassinoides burrows went into complete three‐dimensional boxworks. At the end of the stage, bioturbation almost disappeared, which indicates that burrowers were finding it difficult to survive in the harsh environment.
High mRNA expression of POU2F3 in small cell lung cancer cell lines predicts the effect of lurbinectedin
Authors: Matsui Shinji , Haruki Tomohiro , Oshima Yuki , Kidokoro Yoshiteru , Sakabe Tomohiko , Umekita Yoshihisa , Nakamura Hiroshige
Published: March 2022

Background

Small cell lung cancer (SCLC) is a progressive disease with a poor prognosis. Recently, a method to classify SCLC by the expression status of four transcription factors, ASCL1, NEUROD1, POU2F3, and YAP1, was proposed. Here, we investigated the potential relationships between expression of these four transcription factors and the effect of lurbinectedin.

Methods

mRNA and protein expression of ASCL1, NEUROD1, POU2F3, and YAP1 were quantified in eight SCLC cell lines and analyzed for potential correlations with drug sensitivity. In addition, ASCL1, NEUROD1, POU2F3, and YAP1 expression were evaluated in 105 resected cases of high‐grade neuroendocrine carcinoma of the lung, including 59 resected cases of SCLC.

Results

Based on the results of qRT‐PCR and western blot analyses, the eight SCLC cell lines examined were classified into NEUROD1, POU2F3, and YAP1 subtypes, as well as five ASCL1 subtypes. There were no correlations between cell line subtype classification and drug sensitivity to cisplatin, etoposide, or lurbinectedin. Next, we compared relative mRNA expression levels of each transcription factor with drug sensitivity and found that the higher the mRNA expression level of POU2F3, the lower the IC50 of lurbinectedin. Evaluation of resected SCLC tissue revealed that the composition of subtypes defined by the relative dominance of ASCL1, NEUROD1, POU2F3, and YAP1 was as follows: 61% ASCL1, 15% NEUROD1, 14% POU2F3, 5% YAP1, and 5% all‐negative.

Conclusion

In our experiments, high mRNA expression of POU2F3 in SCLC cell lines correlated with the effect of lurbinectedin.