bluuebunny/crossref_metadata_2025 huggingface dataset:
Health Problems of Students during Distance Learning in Central and Eastern Europe: A Cross-Sectional Study of Poland and Ukraine
Authors: Długosz Piotr , Liszka Damian , Bastrakova Anastasiia , Yuzva Luydmila
Published: August 2022

The COVID-19 pandemic has significantly disrupted the functioning of society. Issues of deteriorating health were among the main problems resulting from restrictions such as self-isolation, social distancing, and remote education. The aim of this research was to attempt to probe the psychophysical condition of students after more than a year of remote education. The survey method (CAPI) was used to collect the data on a representative sample of 1000 students in Poland and 1022 in Ukraine. The research sample was selected in a randomly stratified manner, taking into account such characteristics as: gender, age, and place of residence. The results of the research showed that 44% of Polish and 50% of Ukrainian students experienced health problems. The burden of remote education mainly contributed to the reduction of physical fitness. Young Poles more often paid attention to the deterioration of mental well-being, and Ukrainians to the deterioration of their physical condition. Based on the conducted analyses, it was also established that health problems appeared more often among the females, students with lower social support and with lower trust levels. The main risk factors were Internet addiction, secondary effects of the pandemic, and negative remote education experiences. Research has shown that remote education and problems that arise in students’ households during the pandemic may have significantly contributed to the deterioration of their psychophysical condition.
A Survey of the Challenges Faced by Individuals with Disabilities and Unpaid Caregivers during the COVID-19 Pandemic
Authors: Sharma Yashoda , Whiting Alison , Dutta Tilak
Published: August 2022

The COVID-19 pandemic negatively affected many individuals. In particular, it is likely that individuals with disabilities and unpaid caregivers were disproportionately affected, however, its exact impact is largely unknown. The primary objective of this work was to identify challenges faced by individuals with disabilities and unpaid caregivers. A secondary objective was to identify potential solutions to the major challenges experienced by both populations. Two surveys were administered online to individuals with disabilities and unpaid caregivers, respectively between September 2020 and January 2021. We used an inductive thematic analysis within an interpretivist paradigm to analyze survey responses. A total of 111 survey responses were collected amongst both surveys. Separate thematic maps were created for individuals with disabilities and unpaid caregivers, and maps were drawn to compare challenges. Potential solutions to mitigate the challenges experienced by both populations include revising financial assistance programs and improving awareness of support programs that are available.
How Does Adjacent Land Use Influence Sediment Metals Content and Potential Ecological Risk in the Hongze Lake Wetland?
Authors: Guo Yanhui , Xu Yongfeng , Zhu Chenming , Li Pingping , Zhu Yongli , Han Jiangang
Published: August 2022

Metal pollution in lake wetlands has become increasingly serious in China and worldwide due to the rapid growth of urbanization and agricultural activities. However, comprehensive assessments of metal pollution in lake wetland sediments that are associated with land use change have been limited from an international perspective. Metal concentrations (As, Cd, Cr, Cu, Hg, Mn, Pb, and Zn) were measured in the surface soils and surrounding sediments of five land use types in the eastern Hongze Lake wetlands, including Farmland (FL), Culture Ponds (CP), Reed Land (RL), Poplar Forests (PF), and Willow Forests (WF). The metal pollution status was assessed using the geo-accumulation index and the potential ecological risk index; The results showed that the average concentrations of As, Cd, Mn, and Zn in the surface soils and As, Cd, Cu, and Zn in the sediments, exceeded the background values of Jiangsu Province, China. The FL soils and surrounding sediments were moderately contaminated with As, whereas the sediments surrounding the CP were uncontaminated to moderately contaminated with Cd. Metal pollution in both soils and sediments was greater on farmland than on other types of land use. Furthermore, there were significant positive correlations between the values of the soil risk index and the values of the surrounding sediment risk index. Correlation analysis (CA) and principal component analysis (PCA) found that metals may be derived from agricultural activities such as the application of chemical and organic fertilizers, as well as domestic sewage, industrial wastewater, and geological anomalies. These findings shed new light on the quantitative impacts of adjacent land use practices on sediment metal pollution and provide a scientific foundation for wetland management decision-making.
Bibliometric Analysis of Research on the Use of the Nine Hole Peg Test
Authors: Moreno-Morente Gema , Hurtado-Pomares Miriam , Terol Cantero M. Carmen
Published: August 2022

Manual dexterity is essential for performing daily life tasks, becoming a primary means of interaction with the physical, social, and cultural environment. In this respect, the Nine Hole Peg Test (NHPT) is considered a gold standard for assessing manual dexterity. Bibliometrics is a discipline that focuses on analyzing publications to describe, evaluate, and predict the status and development trends in certain fields of scientific research. We performed a bibliometric analysis to track research results and identify global trends regarding the use of the NHPT. The bibliographic data were retrieved from the Web of Science database and then analyzed using the Bibliometrix R package, resulting in the retrieval of a total of 615 publications from 1988 to 2021. Among the 263 journals investigated, the most prolific were the Multiple Sclerosis Journal, Clinical Rehabilitation, and Multiple Sclerosis and Related Disorders. North America and Europe were the areas with the highest production of publications, with the United States (n = 104) ranking first in terms of the number of publications, followed by the United Kingdom (n = 62) and Italy (n = 62). The analysis of keywords revealed that there were two main lines of research, with one related to the study of recovery and disability of the upper limbs caused by certain diseases and another related to the study of reliability and validity. Structured information can be useful to understand the research trajectory and the uses of this tool.
Spatiotemporal Pattern Evolution and Driving Factors of Brucellosis in China, 2003–2019
Authors: Xu Li , Deng Yijia
Published: August 2022

Brucellosis is a prevalent zoonotic disease worldwide. However, the spatiotemporal patterns evolution and its driving factors of Brucellosis have not been well explored. In this study, spatiotemporal scan statistics were applied to describe the spatiotemporal pattern of evolution in Brucellosis from 2003 to 2019 in mainland China, and GeoDetector analysis was further conducted to explore the driving effects of environmental, meteorological, and socioeconomic factors. We identified a distinct seasonal pattern for Brucellosis, with a peak in May and lowest incidence between September and December. High-risk clusters were first observed in the northwestern pastoral areas and later expanded to the southern urban areas. The spatiotemporal heterogeneity was mainly explained by total SO2 emissions, average annual temperature, sheep output, and consumption of meat per capita with explanatory powers of 45.38%, 44.60%, 40.76%, and 30.46% respectively. However, the explanatory power changed over time. Specifically, the explanatory power of average annual temperature tended to decrease over time, while consumption of meat per capita and total output of animal husbandry tended to increase. The most favorable conditions for the spread of Brucellosis include 0.66–0.70 million tons of SO2 emissions, 9.54–11.68 °C of average annual temperature, 63.28–72.40 million heads of sheep output, and 16.81–20.58 kg consumption of meat per capita. Brucellosis remains more prevalent in traditional pastoral areas in Northwest China, with the tendency of spreading from pastoral to non-pastoral, and rural to urban, areas. Total SO2 emission, average annual temperature, sheep output, and consumption of meat per capita dominated the spatial heterogeneity of Brucellosis with changes in explanatory power over time.
The Impact of the COVID-19 Pandemic on Physicians’ Working Hours and Earnings in São Paulo and Maranhão States, Brazil
Authors: de Oliveira Bruno Luciano Carneiro Alves , Andrietta Lucas Salvador , Reis Regimarina Soares , de Carvalho Ruth Helena de Souza Britto Ferreira , de Britto e Alves Maria Teresa Seabra Soares , Scheffer Mário César , Russo Giuliano
Published: August 2022

Evidence exists on the health impacts of the current COVID-19 pandemic on health workers, but less is known about its impact on their work dynamics and livelihoods. This matters, as health workers—and physicians in particular—are a scarce and expensive resource in low- and middle-income countries (LMICs). Our cross-sectional survey set out to explore changes in working hours and earnings during the second year of the pandemic in a representative sample of 1183 physicians in Brazil’s São Paulo (SP) and Maranhão (MA) states. Descriptive analysis and inferential statistics were employed to explore differences in working hours and earnings among public and private sector physicians across the two locations. The workloads and earnings of doctors working exclusively in the public sector increased the most in the second year of the epidemic, particularly in MA. Conversely, the largest proportion of private-only doctors in our sample saw a decrease in their working hours (48.4%, 95% CI 41.8–55.0), whereas the largest proportion of public-only doctors in MA saw an increase in their working hours (44.4%, 95% CI 38.0–50.8). Although earnings remained broadly stable in the public sector, a third of public sector-only physicians in MA saw an increase in their earnings (95% CI 24.4–36.2). More than half of private-only doctors across both states saw a decrease in their earnings (52.2%, 95% CI 45.6–58.8). The largest proportion of dual practitioners (the majority in Brazil and in our sample) maintained their pre-pandemic levels of income (38.8%, 95% CI 35.3–42.3). As public-sector doctors have been key in the fight against the pandemic, it is critical to invest in these cadres in order to develop epidemic preparedness in LMICs, and to find new ways to harness for-profit actors to deliver social benefits.
Ammunition Waste Pollution and Preliminary Assessment of Risks to Child Health from Toxic Metals at the Greek Refugee Camp Mavrovouni
Authors: Glatz Brubakk Katrin , Gjengedal Elin Lovise Folven , Enger Øyvind , Sripada Kam
Published: August 2022

The Mavrovouni refugee camp near the former Moria camp on the island of Lesvos, Greece, housed approximately 3000 asylum-seekers including children as of October 2021. The camp was built on the site of a military shooting range. This study aimed to characterize the soil contaminants and assess the risk of toxic environmental exposures for children living in Mavrovouni. Methods: Samples of surface soil (0–2 cm depth; particle size < 2 mm) from eight locations inside the camp were compared with two reference samples. Soil samples were microwave digested using a mixture of nitric and hydrofluoric acids and analyzed for lead (Pb), antimony (Sb), bismuth (Bi), and other metals using inductively coupled plasma mass spectrometry. These values were compared with action limits established by the Norwegian Environment Agency for kindergartens, playgrounds, and schools. Findings: Five of eight soil samples from inside the camp exceeded Pb levels of 100 mg/kg, which is currently the maximum acceptable value of Pb in soil for playgrounds in Norway. Two sites had extreme soil Pb levels of approximately 8000 mg/kg and 6000 mg/kg. The concen-tration of Sb and Bi in the surface soil of the firing range area strongly indicated environmental contamination, most likely from previous military activity and ammunition residue that has re-mained on the surface soil. Concentrations of arsenic (As), cadmium (Cd), copper (Cu), and zinc (Zn) in surface soil were lower than action limits. Discussion: Extremely high levels of Pb, together with high levels of Sb and Bi, were identified in soil where children live and play in the Mavrovouni refugee camp. This is the first independent study of environmental contamination at this camp and adds to the limited evidence base documenting Pb exposures prior to migrant and refugee reset-tlement. On top of the multiple existing public health crises and traumas that these asylum-seeking families face, exposure to toxic ammunition residues may have profound impacts on children’s development and health for years to come.
Assessment of Malnutrition in Heart Failure and Its Relationship with Clinical Problems in Brazilian Health Services
Authors: Barbosa Juliana Santos , Souza Márcia Ferreira Cândido de , Costa Jamille Oliveira , Alves Luciana Vieira Sousa , Oliveira Larissa Marina Santana Mendonça de , Almeida Rebeca Rocha de , Oliveira Victor Batista , Pereira Larissa Monteiro Costa , Rocha Raysa Manuelle Santos , Costa Ingrid Maria Novais Barros de Carvalho , Vieira Diva Aliete dos Santos , Baumworcel Leonardo , Almeida-Santos Marcos Antonio , Oliveira Joselina Luzia Menezes , Neves Eduardo Borba , Díaz-de-Durana Alfonso López , Merino-Fernández María , Aidar Felipe J. , Sousa Antônio Carlos Sobral
Published: August 2022

Malnutrition in heart failure (HF) is frequent and associated with a worse prognosis. Due to differences in investment and the profile of those assisted, the objective of this study was to evaluate the frequency of malnutrition in hospitalized patients with HF and its association with clinical outcomes in the public and private health systems. Methodology: A cross-sectional study, with 247 volunteers hospitalized with HF in three public hospitals and one private hospital in Aracaju, SE, Brazil. A subjective global nutritional assessment (SGA) and mini nutritional assessment (MNA) were performed. Results: Sample with 72.5% users of the public health system and 75.3% with malnutrition (public = 74.9%; private = 76.5%; p = 0.793). Regardless of the healthcare system, hospital stay (>14 days) was longer (p = 0.020) among those with malnutrition (48.4%) than well-nourished patients (29.5%). Malnutrition in the public system had higher mortality (7.5%; 5.8%; p < 0.001) and hospital transfer rate (21.1%; 0.0%; p < 0.001) than those in the private system. Death after discharge was observed only in the public system (p = 0.039). Conclusion: Malnutrition was frequent in both systems and was associated with longer hospital stays and, in the public hospital, in-hospital death and transfers.
Vaccination against SARS-CoV-2 in Haemodialysis Patients: Spike’s Ab Response and the Influence of BMI and Age
Authors: Ponce Pedro , Peralta Ricardo , Felix Carla , Pinto Carla , Pinto Bruno , Matos João Fazendeiro
Published: August 2022

Patients with chronic kidney disease (CKD-5D) in dialysis have been associated with higher rates of SARS-CoV-2 infection. Objective: To identify the CKD-5D patients’ immune system behavior regarding the Pfizer-BioNTech (BNT162b2 mRNA) vaccine (Comirnaty©). This was a multicenter study carried out in 38 dialysis units in NephroCare Portugal. Eligible patients from two cohorts—one composed of completely vaccinated patients with Comirnaty© (vaccinated group) against a second cohort of patients who recovered from SARS-CoV-2 infection (control group)—were selected through representative sampling for each cohort. Humoral response was assessed at 3 (t0) and 6 months (t1) after complete vaccination and, in the control group, 6 months after COVID-19 recovery. In the vaccinated group, at t0, the median anti-Spike IgG level was 1120 AU/mL and, at t1, all participants’ antibody level decreased to a median of 455 AU/mL. In the control group, the median serum SARS-CoV-2 antibodies level was 1836 AU/mL. In the vaccinated group, at t0, patients < 70 years presented a significantly (p = 0.002) higher level of anti-Spike IgG titres. In contrast, older patients from the control group presented a significantly (p = 0.038) higher IgG. No correlation was found between age and anti-Spike IgG antibodies level in any of the studied groups. Patients with a higher body mass index showed a greater immune response in both the vaccinated and control group, although without significance. We concluded that, in the vaccinated group, elderly patients developed a lower immune response than younger patients and the levels of anti-Spike IgG antibodies declined faster between t0 and t1, while in the control group, the oldest and overweight patients developed the best humoral response.
The Coronavirus (COVID-19) Pandemic and Mental Health of African Immigrants in the United States
Authors: Yusuf Korede Kafayat , Madu Ednah , Kutchava Salome , Liu Sun Kei
Published: August 2022

The impact of the COVID-19 pandemic on immigrants in the United States is understudied. We investigated the effect of the pandemic on the mental health of African immigrants in the United States and if subjective religiosity was a protective factor. We analyzed primary data collected using an online survey (n = 260). The study’s outcome variable was incident mental health disorder associated with the pandemic, and the primary independent variable was religiosity. Chi-squared and Mann–Whitney U tests were used to compare the distribution of variables by incident mental health disorders. Logistic regression models were used to quantify the association between predictors and the outcome. There were 39 (15.0%) new cases of mental health disorders related to the pandemic. The median scores in the three domains of religiosity of those who developed a mental health disorder and those who did not were not significantly different. Significant predictors of this outcome included having a strong religious support network and loss of household employment income. African immigrants experienced an increased rate of mental health disorders during the pandemic. Implementing community-based strategies to provide social support during a disaster may be essential in promoting mental health in the African immigrant community.
Distrustful, Dissatisfied, and Conspiratorial: A Latent Profile Analysis of COVID-19 Vaccination Rejection
Authors: Lamot Monika , Kerman Katja , Kirbiš Andrej
Published: August 2022

Trust in science and scientists, satisfaction with the national government, and endorsement of conspiracy theories are important factors in the decision to be vaccinated. In this study, we investigated whether there are different profiles of individuals depending on the above factors and whether they differ in their sociodemographic composition. We used data from Round 10 of the European Social Survey for Slovenia, employing a nationally representative sample of 1252 participants. Based on latent profile analysis, three distinct profiles emerged: Profile 1 expressed moderate trust in science, satisfaction with government, and high endorsement of conspiracies; Profile 2 expressed low trust and satisfaction and moderate endorsement of conspiracies; Profile 3 expressed high trust and satisfaction and low beliefs in conspiracy theories. In addition, Profile 3 expressed the strongest support for vaccination and Profile 2 the lowest. Our results suggest that distrust, dissatisfaction, and the presence of conspiracy theories are the “perfect storm” for vaccination rejection. In contrast, despite conspiracy theories, a certain level of trust and satisfaction may reduce vaccination rejection.
Psychological Distress among Hospitalized COVID-19 Patients in Denmark during the First 12 Months of the Pandemic
Authors: Moseholm Ellen , Midtgaard Julie , Bollerup Signe , Apol Ása D. , Olesen Oskar B. , Jespersen Sofie , Weis Nina
Published: August 2022

This study aimed to investigate psychological distress among patients hospitalized with a COVID-19 diagnosis in Denmark during the first 12 months of the pandemic and to assess changes in psychological distress in the three months following discharge. A single-center prospective observational survey study among patients hospitalized with a COVID-19 diagnosis between May 2020 and May 2021 was conducted. Participants completed a survey at three time points: at admission, and 1 and 3 months after discharge. Psychological distress was assessed by validated scales measuring symptoms related to depression, anxiety, stress, insomnia, post-traumatic stress disorder (PTSD), and health-related quality of life (HRQoL). In total, 95 patients were included. At admission, the proportion of patients with symptoms of depression was 43%, symptoms of anxiety 32%, moderate/high level of stress 39%, insomnia 52%, and probable/positive PTSD 19%. The burden of symptoms related to depression and anxiety decreased significantly over time, while there was no significant change over time in stress, insomnia, or PTSD. Suboptimal levels of physical and mental HRQoL were detected at admission but improved over time. Patients hospitalized due to COVID-19 during the first year of the pandemic experienced considerable levels of psychological distress at admission, with some improvements within 3 months after discharge.
Fast Tacrolimus Metabolism Does Not Promote Post-Transplant Diabetes Mellitus after Kidney Transplantation
Authors: Jehn Ulrich , Wiedmer Nathalie , Boeckel Göran Ramin , Pavenstädt Hermann , Thölking Gerold , Reuter Stefan
Published: August 2022

Post-transplant diabetes mellitus (PTDM) after kidney transplantation induced by tacrolimus is an important issue. Fast tacrolimus metabolism, which can be estimated by concentration-to-dose (C/D) ratio, is associated with increased nephrotoxicity and unfavorable outcomes after kidney transplantation. Herein, we elucidate whether fast tacrolimus metabolism also increases the risk for PTDM. Data from 596 non-diabetic patients treated with tacrolimus-based immunosuppression at the time of kidney transplantation between 2007 and 2015 were retrospectively analyzed. The median follow-up time after kidney transplantation was 4.7 years (IQR 4.2 years). Our analysis was complemented by experimental modeling of fast and slow tacrolimus metabolism kinetics in cultured insulin-producing pancreatic cells (INS-1 cells). During the follow-up period, 117 (19.6%) patients developed PTDM. Of all patients, 210 (35.2%) were classified as fast metabolizers (C/D ratio < 1.05 ng/mL × 1/mg). Fast tacrolimus metabolizers did not have a higher incidence of PTDM than slow tacrolimus metabolizers (p = 0.496). Consistent with this, insulin secretion and the viability of tacrolimus-treated INS-1 cells exposed to 12 h of tacrolimus concentrations analogous to the serum profiles of fast or slow tacrolimus metabolizers or to continuous exposure did not differ (p = 0.286). In conclusion, fast tacrolimus metabolism is not associated with increased incidence of PTDM after kidney transplantation, either in vitro or in vivo. A short period of incubation of INS-1 cells with tacrolimus using different concentration profiles led to comparable effects on cell viability and insulin secretion in vitro. Consistent with this, in our patient, collective fast Tac metabolizers did not show a higher PTDM incidence compared to slow metabolizers.
Diagnostic Potential of Circulating Tumor Cells, Urinary MicroRNA, and Urinary Cell-Free DNA for Bladder Cancer: A Review
Authors: Koguchi Dai , Matsumoto Kazumasa , Shiba Izuru , Harano Takahiro , Okuda Satoshi , Mori Kohei , Hirano Shuhei , Kitajima Kazuki , Ikeda Masaomi , Iwamura Masatsugu
Published: August 2022

Early detection of primary bladder cancer (BCa) is vital, because stage and grade have been generally accepted not only as categorical but also as prognostic factors in patients with BCa. The widely accepted screening methods for BCa, cystoscopy and urine cytology, have unsatisfactory diagnostic accuracy, with high rates of false negatives, especially for flat-type BCa with cystoscopy and for low-risk disease with urine cytology. Currently, liquid biopsy has attracted much attention as being compensatory for that limited diagnostic power. In this review, we survey the literature on liquid biopsy for the detection of BCa, focusing on circulating tumor cells (CTCs), urinary cell-free DNA (ucfDNA), and urinary microRNA (umiRNA). In diagnostic terms, CTCs and umiRNA are determined by quantitative analysis, and ucfDNA relies on finding genetic and epigenetic changes. The ideal biomarkers should be highly sensitive in detecting BCa. Currently, CTCs produce an unfavorable result; however, umiRNA and ucfDNA, especially when analyzed using a panel of genes, produce promising results. However, given the small cohort size in most studies, no conclusions can yet be drawn about liquid biopsy’s immediate application to clinical practice. Further large studies to validate the diagnostic value of liquid biopsy for clinical use are mandatory.
Low Light Stress Increases Chalkiness by Disturbing Starch Synthesis and Grain Filling of Rice
Authors: Li Qiuping , Deng Fei , Zeng Yuling , Li Bo , He Chenyan , Zhu Youyun , Zhou Xing , Zhang Zinuo , Wang Li , Tao Youfeng , Zhang Yu , Zhou Wei , Cheng Hong , Chen Yong , Lei Xiaolong , Ren Wanjun
Published: August 2022

Low light stress increases the chalkiness of rice; however, this effect has not been fully characterized. In this study, we demonstrated that low light resulted in markedly decreased activity of ADP-glucose pyrophosphorylase in the grains and those of sucrose synthase and soluble starch synthase in the early period of grain filling. Furthermore, low light also resulted in decreased activities of granule-bound starch synthase and starch branching enzyme in the late period of grain filling. Therefore, the maximum and mean grain filling rates were reduced but the time to reach the maximum grain filling rates and effective grain filling period were increased by low light. Thus, it significantly decreased the grain weight at the maximum grain filling rate and grain weight and retarded the endosperm growth and development, leading to a loose arrangement of the amyloplasts and an increase in the chalkiness of the rice grains. Compared to the grains at the top panicle part, low light led to a greater decrease in the grain weight at the maximum grain filling rate and time to reach the grain weight at the maximum grain filling rate at the bottom panicle part, which contributed to an increase in chalkiness by increasing the rates of different chalky types at the bottom panicle part. In conclusion, low light disturbed starch synthesis in grains, thereby impeding the grain filling progress and increasing chalkiness, particularly for grains at the bottom panicle part.
Genome-Wide Super-Enhancer-Based Analysis: Identification of Prognostic Genes in Oral Squamous Cell Carcinoma
Authors: Saito Tomoaki , Asai Shunichi , Tanaka Nozomi , Nohata Nijiro , Minemura Chikashi , Koma Ayaka , Kikkawa Naoko , Kasamatsu Atsushi , Hanazawa Toyoyuki , Uzawa Katsuhiro , Seki Naohiko
Published: August 2022

Advanced-stage oral squamous cell carcinoma (OSCC) patients are treated with combination therapies, such as surgery, radiation, chemotherapy, and immunotherapy. However, OSCC cells acquire resistance to these treatments, resulting in local recurrence and distant metastasis. The identification of genes involved in drug resistance is essential for improving the treatment of this disease. In this study, we applied chromatin immunoprecipitation sequencing (ChIP-Seq) to profile active enhancers. For that purpose, we used OSCC cell lines that had been exposed to cetuximab for a prolonged period. In total, 64 chromosomal loci were identified as active super-enhancers (SE) according to active enhancer marker histone H3 lysine 27 acetylation (H3K27ac) ChIP-Seq. In addition, a total of 131 genes were located in SE regions, and 34 genes were upregulated in OSCC tissues by TCGA-OSCC analysis. Moreover, high expression of four genes (C9orf89; p = 0.035, CENPA; p = 0.020, PISD; p = 0.0051, and TRAF2; p = 0.0075) closely predicted a poorer prognosis for OSCC patients according to log-rank tests. Increased expression of the four genes (mRNA Z-score ≥ 0) frequently co-occurred in TCGA-OSCC analyses. The high and low expression groups of the four genes showed significant differences in prognosis, suggesting that there are clear differences in the pathways based on the underlying gene expression profiles. These data indicate that potential stratified therapeutic strategies could be used to overcome resistance to drugs (including cetuximab) and further improve responses in drug-sensitive patients.
Expression of NUCB2/NESF-1 in Breast Cancer Cells
Authors: Kmiecik Alicja , Ratajczak-Wielgomas Katarzyna , Grzegrzółka Jędrzej , Romanowicz Hanna , Smolarz Beata , Dziegiel Piotr
Published: August 2022

Recently, the expression of NUCB2/NESF-1 has been linked to tumor development. We report NUCB2/NESF-1 expression and its relation to clinicopathological parameters in breast cancer cells. Immunohistochemical reactions were conducted on 446 cases of invasive ductal carcinoma (IDC) and 36 cases of mastopathy. The expression of NUCB2/NESF-1 was also examined at the mRNA and protein levels in breast cancer cell lines. A statistically significant higher level of NUCB2/NESF-1 in IDC cells was noted compared to that in mastopathy samples. The level of NUCB2 expression in the cytoplasm of IDC cells decreased with the increasing degree of tumor malignancy (G). Higher NUCB2 expression was found in tumors with estrogen receptor (ER)-positive and progesterone receptor (PR)-positive phenotypes compared to that in estrogen-receptor-negative and progesterone-receptor-negative cases. Moreover, a higher expression was shown in ER(+) and PR(+) MCF-7 and T47D cell lines compared to that in triple-negative MDA-MB-468 and normal human breast epithelial cells. The analysis of the five-year survival rate indicated that a positive NUCB2/NESF-1 expression in tumor cells was also associated with longer patient survival. The study results suggest that NUCB2/NESF1 may play an important role in malignant transformation and may be a positive prognostic factor in IDC.
3D-Printing to Plan Complex Transcatheter Paravalvular Leaks Closure
Authors: Ciobotaru Vlad , Tadros Victor-Xavier , Batistella Marcos , Maupas Eric , Gallet Romain , Decante Benoit , Lebret Emmanuel , Gerardin Benoit , Hascoet Sebastien
Published: August 2022

Background: Percutaneous closure of paravalvular leak (PVL) has emerged as an alternative to surgical management in selected cases. Achieving complete PVL occlusion, while respecting prosthesis function remains challenging. A multimodal imaging analysis of PVL morphology before and during the procedure is mandatory to select an appropriate device. We aim to explore the additional value of 3D printing in predicting device related adverse events including mechanical valve leaflet blockade, risk of device embolization and residual shunting. Methods: From the FFPP registries (NCT05089136 and NCT05117359), we included 11 transcatheter PVL closure procedures from three centers for which 3D printed models were produced. Cardiac CT was used for segmentation for 3D printed models (3D-heartmodeling, Caissargues, France). Technology used a laser to fuse very fine powders (TPU Thermoplastic polyurethane) into a final part-laser sintering technology (SLS) with an adapted elasticity. A simulation on 3D printed model was performed using a set of occluders. Results: PVLs were located around aortic prostheses in six cases, mitral prostheses in four cases and tricuspid ring in one case. The device chosen during the simulation on the 3D printed model matched the one implanted in eight cases. In the three other cases, a similar device type was chosen during the procedures but with a different size. A risk of prosthesis leaflet blockade was identified on 3D printed models in four cases. During the procedure, the occluder was removed before release in one case. In another case the device was successfully repositioned and released. In two patients, leaflet impingement was observed post-operatively and surgical device removal had to be performed. Conclusion: In a case-series of complex transcatheter PVL closure procedures, hands-on simulation testing on 3D printed models proved its usefulness to plan and facilitate these challenging procedures.
Mesenchymal Stem Cells Profile in Adult Atopic Dermatitis and Effect of IL4-IL13 Inflammatory Pathway Inhibition In Vivo: Prospective Case-Control Study
Authors: Campanati Anna , Orciani Monia , Marani Andrea , Di Vincenzo Mariangela , Magi Simona , Gregoriou Stamatios , Diotallevi Federico , Martina Emanuela , Radi Giulia , Offidani Annamaria
Published: August 2022

Atopic dermatitis (AD) is an inflammatory disease that typically begins in childhood and may persist into adulthood, becoming a lifelong condition. The major inflammatory mediators of AD are known to be interleukin IL4 and IL13, so Dupilumab, which is able to inhibit both interleukins by blocking the shared IL4Rα subunit, has become an attractive option for treating AD. Mesenchymal stem cells (MSCs) are involved in the onset and development of AD by secreting specific interleukins. The aim of this study was to isolate MSCs from healthy controls (C-MSCs) and patients with AD before (AD-MSCs T0) and after 16 weeks of treatment with Dupilumab (AD-MSCs T16); to evaluate the expression mainly of IL4 and IL13 and of other inflammatory cytokines in C-MSCs, AD-MSCs at T0 and at T16; and to evaluate the efficacy of Dupilumab on MSCs immunobiology. C- and AD-MSCs (T0, T16) were isolated from skin specimens and characterized; the expression/secretion of IL4 and IL13 was evaluated using immuno-cytochemistry (ICC), indirect immune-fluorescence (IIF) and an ELISA test; secretion of IL2, IL4, IL5, IL6, IL10, IL12, IL13, IL17A, Interferon gamma (IFNγ), Tumor necrosis factor alpha (TNFα), Granulocyte Colony-Stimulating Factor (G-CSF), and Transforming Growth Factor beta1 (TGFβ1) were measured with ELISA. IL13 and IL6 were over-expressed, while IL4 was down-regulated in AD-MSCs at T0 compared to C-MSCs. IL6 and IL13 expression was restored after 16 weeks of Dupilumab treatment, while no significant effects on IL4 expression were noted. Finally, IL2, IL5, IL10, IL12, IL17A, INFγ, TNFα, G-CSF, and TGFβ1 were similarly secreted by C- and AD-MSCs. Although Dupilumab blocks the IL4Rα subunit shared by IL4 and IL13, it is evident that its real target is IL13, and its ability to target IL13 in MSCs reinforces the evidence, already known in differentiated cells, of the central role IL13 rather than IL4 in the development of AD. The inflammatory cascade in AD begins at the mesenchymal level, so an upstream therapeutic intervention, able to modify the immunobiology of atopic MSCs, could potentially change the natural history of the disease.
Advances and Innovations in Ablative Head and Neck Oncologic Surgery Using Mixed Reality Technologies in Personalized Medicine
Authors: Karnatz Nadia , Möllmann Henriette L. , Wilkat Max , Parviz Aida , Rana Majeed
Published: August 2022

The benefit of computer-assisted planning in head and neck ablative and reconstructive surgery has been extensively documented over the last decade. This approach has been proven to offer a more secure surgical procedure. In the treatment of cancer of the head and neck, computer-assisted surgery can be used to visualize and estimate the location and extent of the tumor mass. Nowadays, some software tools even allow the visualization of the structures of interest in a mixed reality environment. However, the precise integration of mixed reality systems into a daily clinical routine is still a challenge. To date, this technology is not yet fully integrated into clinical settings such as the tumor board, surgical planning for head and neck tumors, or medical and surgical education. As a consequence, the handling of these systems is still of an experimental nature, and decision-making based on the presented data is not yet widely used. The aim of this paper is to present a novel, user-friendly 3D planning and mixed reality software and its potential application for ablative and reconstructive head and neck surgery.
Assessing the Impact of a Utility Scale Solar Photovoltaic Facility on a Down Gradient Mojave Desert Ecosystem
Authors: Devitt Dale A. , Apodaca Lorenzo , Bird Brian , Dawyot John P. , Fenstermaker Lynn , Petrie Matthew D.
Published: August 2022

A field study was conducted in the Mojave Desert (USA) to assess the influence of a large photo voltaic facility on heat and water transport into an adjacent creosote (Larrea tridentata) bursage (Ambrosia dumosa) plant community. Air temperature, plant physiological status, soil water in storage and precipitation were monitored over a two to four year period. A service road built 27 years before the construction of the PV facility decoupled the wash system at the site leading to a significant decline in soil moisture, canopy level NDVI values and mid-day leaf xylem water potentials (p < 0.001) down gradient from the PV facility. Measurements along a 900 m gradient suggested that plants closer to where the wash was decoupled were placed under significantly greater stress during the higher environmental demand summer months. Air temperatures measured at three 10 m meteorological towers revealed warmer night time temperatures at the two towers located in close association with the solar facility (Inside Facility—IF and Adjacent to facility—AF), compared to the Down Gradient Control tower (DGC). As the warmer air was displaced down gradient, the temperature front advanced into the creosote—bursage plant community with values 5 to 8 °C warmer along an east west front just north of tower AF. Based on our research in Eldorado Valley, NV, USA, a down gradient zone of about 300 m was impacted to the greatest extent (water and heat), suggesting that the spacing between solar facilities will be a critical factor in terms of preserving high quality habitat for the desert tortoise and other species of concern. Greater research is needed to identify habitat zones acceptable for animal populations (especially the desert tortoise) within areas of high solar energy development and this should be done prior to any fragmentation of the ecosystem.
Genome-Wide Analysis of the NAC Family Associated with Two Paleohexaploidization Events in the Tomato
Authors: Yuan Jiale , Liu Ying , Wang Zhenyi , Lei Tianyu , Hu Yanfang , Zhang Lan , Yuan Min , Wang Jinpeng , Li Yuxian
Published: August 2022

NAC transcription factors play an important regulatory role in tomato fruit ripening. We chose a novel perspective to explore the traces left by two paleopolyploidizations in the NAC family using a bioinformatics approach. We found that 85 (S. lycopersicum) and 88 (S. pennellii) members of the NAC family were present in two tomatoes, and most of them were amplified from two paleohexaploidizations. We differentiated NAC family members from the different paleohexaploidizations and found that the SWGT-derived NAC genes had more rearrangement events, so it was different from the DWGT-derived NAC genes in terms of physicochemical properties, phylogeny, and gene location. The results of selection pressure show that DWGT-derived NAC genes tended to be positively selected in S. lycopersicum and negatively selected in S. pennellii. A comprehensive analysis of paleopolyploidization and expression reveals that DWGT-derived NAC genes tend to promote fruit ripening, and are expressed at the early and middle stages, whereas SWGT-derived NAC genes tend to terminate fruit growth and are expressed at the late stages of fruit ripening. This study obtained NAC genes from different sources that can be used as materials for tomato fruit development, and the method in the study can be extended to the study of other plants.
Deformation Characteristics, Formability and Springback Control of Titanium Alloy Sheet at Room Temperature: A Review
Authors: Li Hao , Chen Shuai-Feng , Zhang Shi-Hong , Xu Yong , Song Hong-Wu
Published: August 2022

Titanium alloy sheets present inferior formability and severe springback in conventional forming processes at room temperature which greatly restrict their applications in complex-shaped components. In this paper, deformation characteristics and formability and springback behaviors of titanium alloy sheet at room temperature are systematically reviewed. Firstly, deformation characteristics of titanium alloys at room temperature are discussed, and formability improvement under high-rate forming and other methods are summarized, especially the impacting hydroforming developed by us. Then, the main advances in springback prediction and control are outlined, including the advanced constitutive models as well as the optimization of processing paths and parameters. More importantly, notable springback reduction is observed with high strain rate forming methods. Finally, potential investigation prospects for the precise forming of titanium alloy sheet in the future are suggested.
A Methodology for Optimizing the Calibration and Validation of Reactive Transport Models for Cement-Based Materials
Authors: Addassi Mouadh , Marcos-Meson Victor , Kunther Wolfgang , Hoteit Hussein , Michel Alexander
Published: August 2022

Reactive transport models are useful tools in the development of cement-based materials. The output of cement-related reactive transport models is primarily regarded as qualitative and not quantitative, mainly due to limited or missing experimental validation. This paper presents an approach to optimize the calibration process of reactive transport models for cement-based materials, using the results of several short-term experiments. A quantitative comparison of changes in the hydrate phases (measured using TGA and XRD) and exposure solution (measured using ICP-OES) was used to (1) establish a representative chemical model, limiting the number of hydrate phases and dissolved species, and (2) calibrate the transport processes by only modeling the initial tortuosity. A case study comprising the early age carbonation of cement is presented to demonstrate the approach. The results demonstrate that the inclusion of a microstructure model in our framework minimizes the impact of the initial tortuosity factor as a fitting parameter for the transport processes. The proposed approach increases the accuracy of reactive transport models and, thus, allowing for more realistic modeling of long-term exposure.
Description of Dynamic Recrystallization Behaviors and Grain Evolution Mechanisms during the Hot Forming Process for SAE 5137H Steel
Authors: Zhang Yu-Qing , Quan Guo-Zheng , Lei Sheng , Zhao Jiang , Xiong Wei
Published: August 2022

Describing the dynamic recrystallization (DRX) behaviors and grain evolution mechanisms in the hot forming process contributes to controlling microstructures and enhancing mechanical properties of materials. Here, the isothermal compression experiments for SAE 5137H steel were conducted under temperatures of 1123–1483 K and strain rates of 0.01–10 s−1. The DRX kinetics models, including DRX volume fraction and grain size models, and the meso-scale cellular automaton (CA) models, were established based on the obtained true stress–strain curves and microstructure observation results. In order to dynamically reveal DRX behaviors and grain morphology evolution, a multi-field and multi-scale coupling finite element (FE) model for the hot compression process was developed by embedding the solved DRX kinetics models and CA models. Results show that the DRX volume fraction and grain size increase with temperature increasing and strain rate decreasing. The DRX grains are easier to nucleate at the initial grain boundaries. As strain increases, DRX grains grow up by devouring the matrix grains until DRX occurs completely. The microstructures after compression are composed of equiaxed DRX grains. Finally, the comparisons of grain size between experimental results and simulation results were performed. The mean relative errors between experimental results and predicted results from DRX kinetics models, and between experimental results and predicted results from CA models, were evaluated as 6.5% and 6.0%, respectively. It proves that the developed FE model can well describe the microstructure evolution in the hot deformation process of SAE 5137H steel.
Energy Channelization Analysis of Rough Tools Developed by RM-MT-EDM Process during ECSM of Glass Substrates
Authors: Singh Tarlochan , Dvivedi Akshay , Sidhu Sarabjeet Singh , Shlykov Evgeny Sergeevich , Muratov Karim Ravilevich , Ablyaz Timur Rizovich
Published: August 2022

In the present work, the effect of tool surface roughness on energy channelization behavior was analyzed during the fabrication of micro holes by an electrochemical spark machining (ECSM) process. In this study, rough tools were fabricated by a rotary mode multi tip electric discharge machining (RM-MT-EDM) process. The electrical characterization was also carried out to investigate the electric field intensity over the surface of tool electrode, and it was found that the use of rough tools improves the electric field intensity by 265.54% in comparison to the smooth tool electrodes. The use of rough tools in the ECSM process forms thin and stable gas film over the tool electrode, and as a result the rough tools produced high frequency spark discharges. Energy channelization index and specific energy were considered as response characteristics. The use of rough tools improves energy channelization index by 248.40%, and the specific energy is reduced by 143.263%. The material removal mechanisms for both of the processes (RM-MT-EDM and ECSM process) have also been presented through illustrations.
Anion Exchange Membranes for Alkaline Polymer Electrolyte Fuel Cells—A Concise Review
Authors: Kuppusamy Hari Gopi , Dhanasekaran Prabhakaran , Nagaraju Niluroutu , Neeshma Maniprakundil , Dass Baskaran Mohan , Dhavale Vishal M. , Unni Sreekuttan M. , Bhat Santoshkumar D.
Published: August 2022

Solid anion exchange membrane (AEM) electrolytes are an essential commodity considering their importance as separators in alkaline polymer electrolyte fuel cells (APEFC). Mechanical and thermal stability are distinguished by polymer matrix characteristics, whereas anion exchange capacity, transport number, and conductivities are governed by the anionic group. The physico-chemical stability is regulated mostly by the polymer matrix and, to a lesser extent, the cationic head framework. The quaternary ammonium (QA), phosphonium, guanidinium, benzimidazolium, pyrrolidinium, and spirocyclic cation-based AEMs are widely studied in the literature. In addition, ion solvating blends, hybrids, and interpenetrating networks still hold prominence in terms of membrane stability. To realize and enhance the performance of an alkaline polymer electrolyte fuel cell (APEFC), it is also necessary to understand the transport processes for the hydroxyl (OH−) ion in anion exchange membranes. In the present review, the radiation grafting of the monomer and chemical modification to introduce cationic charges/moiety are emphasized. In follow-up, the recent advances in the synthesis of anion exchange membranes from poly(phenylene oxide) via chloromethylation and quaternization, and from aliphatic polymers such as poly(vinyl alcohol) and chitosan via direct quaternization are highlighted. Overall, this review concisely provides an in-depth analysis of recent advances in anion exchange membrane (AEM) and its viability in APEFC.