bluuebunny/crossref_metadata_2025 huggingface dataset:
PTCY-Based Haploidentical Donor Transplantation versus HLA-Matched Related and Unrelated Donor Transplantations in Patients with Refractory or Relapsed Lymphoma—A Matched-Pair Analysis
Authors: Haebe Sarah , Fraccaroli Alessia , Stauffer Elena , Prevalsek Dusan , Zoellner Anna K. , Drolle Heidrun , Stemmler Hans-Joachim , Dreyling Martin , von Bergwelt-Baildon Michael , Tischer Johanna
Published: October 2023

Allogeneic hematopoietic stem cell transplantation (allo-HSCT) has demonstrated its potential as a curative option for patients with r/r lymphoma. With the introduction of post-transplant cyclophosphamide-based (PTCY) graft-versus-host disease (GvHD) prophylaxis, allo-HCT using haploidentical related donors (Haplo-HSCT) has emerged as a valuable alternative for patients without an available HLA-matched donor. In this study, we compared intermediate and long-term outcomes between Haplo-HSCT and HLA-matched related donor (MRD) and unrelated donor (URD) transplantations in 16 matched pairs using age, disease status, lymphoma classification and performance status as matching criteria. Of note, 88% of patients in each group presented with active disease at the time of conditioning. After a median follow-up of >10 years, 10-year overall and progression-free survival and non-relapse mortality incidence after Haplo-HSCT were 31%, 25% and 38%, respectively, and did not differ compared to the values observed in MRD-HSCT and URD-HSCT. A remarkable lower incidence of acute GvHD ≥ II and moderate and severe chronic GvHD was observed after Haplo-HSCT compared to MRD-HSCT (50%/50%, p = 0.03/0.03) and URD-HSCT (44%/38%, p = 0.04/0.08), resulting in slightly higher 10-year GvHD-free and relapse-free survival (25%) and chronic GvHD-free and relapse-free survival (25%) in the Haplo-HSCT group. In conclusion, Haplo-HSCT is an effective treatment in patients with non-remission NHL. Given its advantage of immediate availability, haploidentical donors should be preferably used in patients with progressive disease lacking an HLA-matched related donor.
Functional Proteomics Characterization of the Role of SPRYD7 in Colorectal Cancer Progression and Metastasis
Authors: Montero-Calle Ana , Jiménez de Ocaña Sofía , Benavente-Naranjo Ruth , Rejas-González Raquel , Bartolomé Rubén A. , Martínez-Useros Javier , Sanz Rodrigo , Dziaková Jana , Fernández-Aceñero María Jesús , Mendiola Marta , Casal José Ignacio , Peláez-García Alberto , Barderas Rodrigo
Published: October 2023

SPRY domain-containing protein 7 (SPRYD7) is a barely known protein identified via spatial proteomics as being upregulated in highly metastatic-to-liver KM12SM colorectal cancer (CRC) cells in comparison to its isogenic poorly metastatic KM12C CRC cells. Here, we aimed to analyze SPRYD7’s role in CRC via functional proteomics. Through immunohistochemistry, the overexpression of SPRYD7 was observed to be associated with the poor survival of CRC patients and with an aggressive and metastatic phenotype. Stable SPRYD7 overexpression was performed in KM12C and SW480 poorly metastatic CRC cells and in their isogenic highly metastatic-to-liver-KM12SM-and-to-lymph-nodes SW620 CRC cells, respectively. Upon upregulation of SPRYD7, in vitro and in vivo functional assays confirmed a key role of SPRYD7 in the invasion and migration of CRC cells and in liver homing and tumor growth. Additionally, transient siRNA SPRYD7 silencing allowed us to confirm in vitro functional results. Furthermore, SPRYD7 was observed as an inductor of angiogenesis. In addition, the dysregulated SPRYD7-associated proteome and SPRYD7 interactors were elucidated via 10-plex TMT quantitative proteins, immunoproteomics, and bioinformatics. After WB validation, the biological pathways associated with the stable overexpression of SPRYD7 were visualized. In conclusion, it was demonstrated here that SPRYD7 is a novel protein associated with CRC progression and metastasis. Thus, SPRYD7 and its interactors might be of relevance in identifying novel therapeutic targets for advanced CRC.
The Influence of Obesity on Outcomes with Immune Checkpoint Blockade: Clinical Evidence and Potential Biological Mechanisms
Authors: Hahn Andrew W. , Venkatesh Neha , Msaouel Pavlos , McQuade Jennifer L.
Published: October 2023

Immune checkpoint blockade (ICB) is a mainstay of treatment for advanced cancer, yet tumor response and host toxicity are heterogenous in those patients who receive ICB. There is growing interest in understanding how host factors interact with tumor intrinsic properties and the tumor microenvironment to influence the therapeutic index with ICB. Obesity, defined by body mass index, is a host factor associated with improved outcomes in select cancers when treated with ICB. While the biological mechanism for this obesity paradox is not fully understood, pre-clinical and translational studies suggest obesity may potentially impact tumor metabolism, inflammation, and angiogenesis. Herein, we summarize clinical studies that support an obesity paradox with ICB, explore potential biological mechanisms that may account for the obesity paradox, and address methodological challenges to consider when studying obesity and treatment outcomes.
Attenuating Colorectal Cancer Using Nine Cultivars of Australian Lupin Seeds: Apoptosis Induction Triggered by Mitochondrial Reactive Oxygen Species Generation and Caspases-3/7 Activation
Authors: Mazumder Kishor , Aktar Asma , Ramasamy Sujatha , Biswas Biswajit , Kerr Philip G. , Blanchard Christopher
Published: October 2023

As Australian lupin cultivars are rich sources of polyphenols, dietary fibers, high-quality proteins, and abundant bioactive compounds with significant antioxidant, antidiabetic, and anticancer activities, this research work is aimed at investigating the colon cancer alleviation activity of nine cultivars of lupin seeds on HCT116 and HT29 colon carcinoma cell lines through anti-proliferation assay, measurement of apoptosis, and identification of the mechanism of apoptosis. Nine cultivars were pre-screened for anti-proliferation of HCT116 and HT29 cells along with consideration of the impact of heat processing on cancer cell viability. Mandelup and Jurien showed significant inhibition of HCT116 cells, whereas the highest inhibition of HT29 cell proliferation was attained by Jurien and Mandelup. Processing decreased the anti-proliferation activity drastically. Lupin cultivars Mandelup, Barlock, and Jurien (dose: 300 μg/mL) induced early and late apoptosis of colon cancer cells in Annexin V-FITC assay. The mechanism of apoptosis was explored, which involves boosting of caspases-3/7 activation and intracellular reactive oxygen species (ROS) generation in HCT116 cells (Mandelup and Barlock) and HT29 cells (Jurien and Mandelup). Thus, the findings showed that lupin cultivars arrest cell cycles by inducing apoptosis of colorectal carcinoma cells triggered by elevated ROS generation and caspases-3/7 activation.
Safety and Feasibility of Functional Repetitive Neuromuscular Magnetic Stimulation of the Gluteal Muscles in Children and Adolescents with Bilateral Spastic Cerebral Palsy
Authors: Grosse Leonie , Schnabel Julian F. , Börner-Schröder Corinna , Späh Malina A. , Meuche Anne C. , Sollmann Nico , Breuer Ute , Warken Birgit , Hösl Matthias , Heinen Florian , Berweck Steffen , Schröder Sebastian A. , Bonfert Michaela V.
Published: October 2023

Background: For children and adolescents affected by bilateral spastic cerebral palsy (BSCP), non-invasive neurostimulation with repetitive neuromuscular magnetic stimulation (rNMS) combined with physical exercises, conceptualized as functional rNMS (frNMS), represents a novel treatment approach. Methods: In this open-label study, six children and two adolescents (10.4 ± 2.5 years) with BSCP received a frNMS intervention targeting the gluteal muscles (12 sessions within 3 weeks). Results: In 77.1% of the sessions, no side effects were reported. In 16.7%, 6.3% and 5.2% of the sessions, a tingling sensation, feelings of pressure/warmth/cold or very shortly lasting pain appeared, respectively. frNMS was highly accepted by families (100% adherence) and highly feasible (97.9% of treatment per training protocol). A total of 100% of participants would repeat frNMS, and 87.5% would recommend it. The Canadian Occupational Performance Measure demonstrated clinically important benefits for performance in 28% and satisfaction in 42% of mobility-related tasks evaluated by caregivers for at least one follow-up time point (6 days and 6 weeks post intervention). Two patients accomplished goal attainment for one mobility-related goal each. One patient experienced improvement for both predefined goals, and another participant experienced improvement in one and outreach of the other goal as assessed with the goal attainment scale. Conclusions: frNMS is a safe and well-accepted neuromodulatory approach that could improve the quality of life, especially in regard to activity and participation, of children and adolescents with BSCP. Larger-scaled studies are needed to further explore the effects of frNMS in this setting.
Lengthening Patients Previously Treated for Massive Lower Limb Reconstruction for Bone Tumors with the PRECICE 2 Nail
Authors: Campanacci Laura , Cevolani Luca , Focaccia Marco , Di Gennaro Giovanni Luigi , Dozza Barbara , Staals Eric , Zuccheri Federica , Bianchi Giuseppe , Donati Davide Maria , Manfrini Marco
Published: October 2023

The objective of this study was to determine the efficacy of the PRECICE 2® nail in the treatment of lower limb length discrepancy in patients with a history of bone tumors. This study reports on outcomes, complications, and the safety of the PRECICE 2 limb lengthening nail in a cohort of pediatric patients with limb length discrepancy after surgery for bone tumors. Seventeen patients were treated with intramedullary magnetic nails. The average patient age at the time of surgery was 19 (range 11–32). The PRECICE 2 nail was used on 14 femurs (6 retrograde and 8 anterograde) and 3 tibias. The average consolidation time was 141 days (range 50–360) with a mean CI of 31 ± 12 days/cm. The ASAMI bone score showed 14 (82%) excellent results, 1 (6%) good result, and 2 (12%) poor results. The ASAMI functional score showed 13 (84.6%) excellent results, 3 (11.5%) good results, and 1 (3.8%) fair result. Patients treated with chemotherapy for bone cancer did not show any increase in distraction time or consolidation time. A total of 3 (17%) problems, 1 obstacle (5.5%), and 1 complication (5.5%) were encountered in our case series. The PRECICE 2 nail allows for effective and accurate lengthening preserving the range of motion in patients treated for bone tumors.
Influence of Electron Beam Treatment on Structure and Phase Composition of TiB2–Ag Coating Deposited by Electrical Explosion Spraying
Authors: Filyakov Artem D. , Pochetukha Vasilii V. , Romanov Denis A. , Vashchuk Ekaterina S.
Published: October 2023

Due to many factors, the electrical explosion spraying process is not stable, which directly causes unstable coating quality and structure. Electron beam treatment may be used to improve the surface and modified structure of coatings sprayed by electrical explosions. In this study, a new TiB2–Ag metal matrix composite coating was deposited by electrical explosion spraying and modified by electron beam treatment. The prepared coatings were characterized by surface macro- and microanalysis, XDR, cross-section SEM, and TEM. The composition of the spray-coating phase differs from sample to sample. The electron beam treatment normalized the phase composition. Ag TiB2 B2O became the main phase in the modified coating. Increasing the pulse energy density and duration leads to a reduction in the low-melting Ag phase and the formation of copper contact phases due to heating and melting of the copper substrate by excess electron beam energy. The coating structure consists of a silver matrix and TiB2 inclusions. The electron beam treatment did not affect the structure; however, the microstructure of the coating transformed into a cellular crystallization structure. The silver matrix nanostructure was transformed into a nanocrystalline structure with an average crystal size ranging from tens to hundreds of nanometers.
The Optical Properties of Thin Film Alloys of ZnO, TiO2 and ZrO2 with Al2O3 Synthesised Using Atomic Layer Deposition
Authors: Nosidlak Natalia , Jaglarz Janusz , Vallati Andrea , Dulian Piotr , Jurzecka-Szymacha Maria , Gierałtowska Sylwia , Seweryn Aleksandra , Wachnicki Łukasz , Witkowski Bartłomiej S. , Godlewski Marek
Published: October 2023

In this work, the results of ellipsometric studies of thin films of broadband oxides (ZnO, TiO2, ZrO2) and broadband oxides doped with Al2O3 (Al2O3–ZnO, Al2O3–TiO2, Al2O3–ZrO2) are presented. All layers have been produced using the atomic layer deposition method. Ellipsometric studies were performed in the wavelength range of 193–1690 nm. Sellmeier and Cauchy models were used to describe the optical properties of the tested layers. Dispersion dependencies of refractive indices were determined for thin layers of broadband oxides on silicon substrates, and then for layers of Al2O3 admixture. The EDX investigations enabled estimation of the composition of the alloys. The Bruggeman effective medium approximation (EMA) model was used to determine the theoretical dependencies of the dispersion refractive indices of the studied alloys. The refractive index values determined using the Bruggeman EMA model are in good agreement with the values determined from the ellipsometric measurements. The doping of thin layers of ZnO, ZrO2 and TiO2 with Al2O3 enables the creation of anti-reflective layers and filters with a specific refractive index.
Construction of Flower-like FeCo2O4 Nanosheets on Ni Foam as Efficient Electrocatalyst for Oxygen Evolution Reaction
Authors: Zhang Lijuan , Quan Zhonggui , Wang Yan , Li Hangyang , Yang Xu
Published: October 2023

Developing efficient transition metal oxide electrodes is essential to energy conversion and storage. In this work, flower-Like FeCo2O4 nanosheets supported on Ni foam were synthesized by facile hydrothermal and calcination treatment. Various temperatures influence the morphologies and oxygen evolution reaction activities. Especially, FeCo2O4/NF-120 °C catalysts showed the best oxygen evolution reaction (OER) activity due to the fact that 3D Ni foam provided good conductive substrate-forming FeCo2O4 nanosheets, which enhanced the electrochemical stability and facilitated the transport of electrolyte and release of oxygen. In addition, the synergistic effect between Fe and Co also enhanced active sites and promoted the OER catalytic performance. The flower-like FeCo2O4/Ni electrodes showed a low overpotential of 124 and 339 mV at the current density of 10 and 50 mA cm−2 for OER, respectively. Also, they displayed a low tafel slope of 43.78 mV dec−1 and good stability in alkaline electrolyte. This research could promote the design of low-cost electrocatalysts for OER.
Preclinical Synergistic Combination Therapy of Lurbinectedin with Irinotecan and 5-Fluorouracil in Pancreatic Cancer
Authors: Tummala Tej , Sevilla Uruchurtu Ashley Sanchez , Cruz Arielle De La , Huntington Kelsey E. , George Andrew , Liguori Nicholas R. , Zhang Leiqing , Zhou Lanlan , Abbas Abbas E. , Azzoli Christopher G. , El-Deiry Wafik S.
Published: October 2023

Pancreatic cancer is a devastating disease with a poor prognosis. Novel chemotherapeutics in pancreatic cancer have shown limited success, illustrating the urgent need for new treatments. Lurbinectedin (PM01183; LY-01017) received FDA approval in 2020 for metastatic small cell lung cancer on or after platinum-based chemotherapy and is currently undergoing clinical trials in a variety of tumor types. Lurbinectedin stalls and degrades RNA Polymerase II and introduces breaks in DNA, causing subsequent apoptosis. We now demonstrate lurbinectedin’s highly efficient killing of human-derived pancreatic tumor cell lines PANC-1, BxPC-3, and HPAF-II as a single agent. We further demonstrate that a combination of lurbinectedin and irinotecan, a topoisomerase I inhibitor with FDA approval for advanced pancreatic cancer, results in the synergistic killing of pancreatic tumor cells. Western blot analysis of combination therapy indicates an upregulation of γH2AX, a DNA damage marker, and the Chk1/ATR pathway, which is involved in replicative stress and DNA damage response. We further demonstrate that the triple combination between lurbinectedin, irinotecan, and 5-fluorouracil (5-FU) results in a highly efficient killing of tumor cells. Our results are developing insights regarding molecular mechanisms underlying the therapeutic efficacy of a novel combination drug treatment for pancreatic cancer.
The Predictive Value of CD3+/CD8+ Lymphocyte Infiltration and PD-L1 Expression in Colorectal Cancer
Authors: Liu Jihong , Li Jinbang , Luo Feng , Wu Shigang , Li Bingquan , Liu Kunping
Published: October 2023

Aim: The immune system plays an important role in tumor development and treatment. In this study, we aimed to determine the relationships among the expressions of PD-L1, CD3, CD8, MMR proteins, clinicopathological features, and prognosis of CRC. Methods: Immunohistochemistry was used to determine the expression of PD-L1, CD3, and CD8 in 771 patients with CRC. Results: The expression of PD-L1 in TC was related to the right colon, adenocarcinoma, and dMMR, and in IC, it was related to younger CRC patients and the TNM stage. The expression of CD3 and CD8 in tumor-infiltrating lymphocytes was related to lymph node metastasis and the TNM stage. The expression of PD-L1 in TC and IC was correlated with the infiltration of CD3+ and CD8+ lymphocytes. Univariate survival analysis showed that the expression of PD-L1 in TC, IC, and dMMR was related to a better prognosis. Multivariate survival analysis showed that age, TNM stage, and dMMR were independent prognostic factors for CRC. The OS of the chemotherapy was significantly higher than that of the non-chemotherapy in III-IV TNM stage patients; CRC patients with positive PD-L1 expression in TC or IC and dMMR did not benefit from chemotherapy. Conclusions: PD-L1 expression in TC and IC was closely related to the density of CD3 and CD8 infiltration in tumor-infiltrating lymphocytes. The expression of CD3 and CD8 in tumor-infiltrating lymphocytes and the expression of PD-L1 in IC were linked to the TNM stage of CRC patients. PD-L1 expression in TC and IC and MMR status may act as an important biomarker for guiding the postoperative treatment of III-IV TNM stage CRC patients.
The Phylogenetic Relationships of Australian Species within Charopidae (Gastropoda: Punctoidea)
Authors: Colgan Donald James , Stanisic John
Published: October 2023

Charopidae is a highly speciose family of land snails mostly distributed in the main landmasses and archipelagos of the Southern Hemisphere, with a few genera in the Northern Hemisphere. DNA sequencing is making substantial contributions to understanding the phylogeography of Charopidae and the systematic relationships of its subfamilies and related families in the Punctoidea. Here, new data from 28S rRNA are combined with available GenBank sequences to investigate whether Australian Charopidae comprise a single clade, exclusive of species from other regions. Phylogenetic analyses show that this is not the case, with most species from Australia and other regions included in a bootstrap-supported clade that excludes some Australian species. Analyses of concatenated 28S rRNA data and available cytochrome c oxidase subunit I (COI) also contradicted monophyly of Australian species, as did analyses of inferred COI protein sequences. In DNA analyses, the genus Hirasea and Australian taxa with an incomplete secondary ureter were basal within Charopidae. We also examined whether known doubts about the taxonomic status of supra-generic clades in Punctoidea could be resolved by considering autapomorphic COI amino acid changes. Such variation could not generally be applied as an objective criterion for taxonomic categorization but did identify some clades for taxonomic investigation.
Correlation of HbA1c Level with Lipid Profile in Type 2 Diabetes Mellitus Patients Visiting a Primary Healthcare Center in Jeddah City, Saudi Arabia: A Retrospective Cross-Sectional Study
Authors: Sharahili Abdulaziz Yahya , Mir Shabir Ahmad , ALDosari Sahar , Manzar Md Dilshad , Alshehri Bader , Al Othaim Ayoub , Alghofaili Fayez , Madkhali Yahya , Albenasy Kamal Shaker , Alotaibi Jazi S.
Published: October 2023

Introduction: Type 2 diabetes mellitus (T2DM) patients are at high risk of dyslipidemia, which in turn is associated with macrovascular diseases, such as heart diseases and stroke, and microvascular diseases, such as neuropathy and nephropathy. There are contradictory findings in the literature regarding the relationship between glycated hemoglobin (HbA1c) and the lipid profile among T2DM patients. This study was performed to investigate the association between HbA1c level and the lipid profile in elderly T2DM patients at a primary care hospital in Jeddah City, Saudi Arabia. Methods: This study is a retrospective cross-sectional study conducted at the Prince Abdul Majeed Healthcare Center (PAMHC) in Jeddah, Saudi Arabia. The sociodemographic and clinical data of the T2DM patients who had visited the PAMHC from 1 January 2020 to 31 December 2021, were collected from the data registry of the PAMHC and analyzed for publication. Results: The study included a total of 988 T2DM patients (53.3% male). Of the participants, 42.9% were aged between 55 and 64 years. Dyslipidemia parameters were presented as high LDL-c (in 60.3% cases), low HDL-c (in 39.8% cases), high triglycerides (in 34.9% cases), and high total cholesterol (in 34.8% cases). The correlation of HbA1c with total cholesterol (TC) and triglycerides (TGs) was positively significant, thereby highlighting the important link between glycemic control and dyslipidemia. A mean increase of 4.88 mg/dL and 3.33 mmHg in TG level and diastolic blood pressure, respectively, was associated with the male gender, in comparison to the female gender. However, the male gender was significantly associated with the reduction in the mean cholesterol level, BMI, HbA1c, HDL-c, and LDL-c by 11.49 mg/dL, 1.39 kg/m2, 0.31%, 7.47 mg/dL, and 5.6 mg/dL, respectively, in comparison to the female gender. Conclusions: The results of this study show that HbA1c was significantly associated with cholesterol and triglyceride levels in the T2DM patients included in the study. Our findings highlight the important relationship between glycemic control and dyslipidemia.
Competition and Cooperation for Multiple Solar Powered Unmanned Aerial Vehicles under Static Soaring
Authors: Wu Yansen , Li Ke , Zhao Anmin , Wang Shaofan , Li Yuangan , Chen Xiaodan
Published: October 2023

This work examines the competition and allocation of multiple solar-powered unmanned aerial vehicles (SUAVs) to a single thermal since multiple SUAVs often demonstrate superior mission performance compared to a single SUAV. Additionally, they can harvest extra energy from thermal updrafts. This work considers two conditions, a non-cooperative competition and a cooperative allocation of thermal. In each case, corresponding objective functions and constraints are established, and assignment schemes are derived by solving these objective functions. The allocation results are simulated and integrated with the dynamics and solar energy model. The numerical results show that, in the non-cooperative mode, the first vehicle to reach the thermal can occupy it for soaring, while the remaining SUAVs will fly towards the destination directly. But in the cooperative mode, the multiple SUAVs will allocate the thermal to the SUAV with the highest energy gain through soaring, to maximize the overall electric energy storage of the SUAV group.
Fear of COVID-19, Perceived Stress, and PTSD: The Serial Mediating Role of Sense of Coherence
Authors: Padmanabhanunni Anita , Pretorius Tyrone Brian
Published: October 2023

The literature has identified that a sense of coherence plays a protective role in the relationship between adverse events and mental health. The current study examines the role of a sense of coherence (SOC) in the relationship between fear of COVID-19, perceived stress, and dimensions of post-traumatic stress disorder (PTSD). Participants (n = 322) were students at a metropolitan university in South Africa who completed the Fear of COVID-19 Scale, the Perceived Stress Scale, the 13-item Sense of Coherence Scale, and the PTSD Checklist. Path analysis was used to conduct a serial mediation analysis. The results show that SOC mediates the relationship between perceived stress and the dimensions of PTSD but does not mediate the relationship between fear of COVID-19 and PTSD. Furthermore, the relationship between fear of COVID-19 and dimensions of PTSD was mediated by serial perceived stress and sense of coherence, supporting the hypothesis that higher levels of fear of COVID-19 leads to higher levels of perceived stress. However, while high levels of fear of COVID-19 increase perceived stress, SOC significantly mediates the subsequent impact on PTSD symptoms.
Foraging Niche Differentiation of Five Woodpecker Species in the Primitive Broadleaved Korean Pine Forests of Northeast China
Authors: Si Yuhui , Meng Dehuai , Zhong Han , Zhu Ziwei , Zou Hongfei , Rong Ke
Published: October 2023

Explaining the mechanism of the coexistence of sympatric species is an important goal of ecology. Five species of woodpeckers coexist in the broadleaved Korean pine forest of Liangshui National Nature Reserve, including the Black Woodpecker (Dryocopus martius), Great Spotted Woodpecker (Dendrocopos major), Lesser Spotted Woodpecker (Dendrocopos minor), Three-toed Woodpecker (Picoides tridactylus), and White-backed Woodpecker (Dendrocopos leucotos). Woodpeckers are considered to be keystone species because of their role as ecosystem engineers, creating breeding and shelter sites for many vertebrate and invertebrate taxa. As woodpeckers are predominant in primary forests, they are sensitive to changes in forest ecosystems. To understand their coexistence mechanisms and propose conservation strategies, it is necessary to investigate their foraging niche differentiation. This study aimed to identify the foraging behavior parameters and foraging tree parameters of five woodpecker species in Liangshui Reserve from October to December. The foraging niches of five woodpecker species were observed, including the type of foraging techniques, foraging height, foraging site, foraging duration, tree species being foraged upon, diameter of the foraging tree at breast height, foraging tree height, and decay status of trees. Our results identified that there were significant differences in the overall foraging ecology of the five species of woodpecker at Liangshui Reserve. The Great Spotted Woodpecker and Lesser Spotted Woodpecker had more diverse foraging patterns and preferred to forage on live trees. The Black Woodpecker and Three-toed Woodpecker excavated and pecked at the trunks of decaying spruce and fir trees. The White-backed Woodpecker preferred to forage in broadleaved trees. The choice of foraging sites was complicated. The size of the foraging trees and decay status of trees were important bases for woodpeckers when choosing trees to forage from. Different woodpeckers achieve stable coexistence through the separation of their foraging niches. This information regarding foraging behavior and foraging tree characteristics provides a basis to study the coexistence patterns of woodpeckers. Our research into woodpecker foraging should be used to inform forest management practices, protect forest ecosystem diversities, and maintain woodpecker community diversity.
Improvement of Laccase Activity in Co-Culture of Panus lecomtei and Sporidiobolus pararoseus and Its Application as an Enzymatic Additive in Biomass Hydrolysis and Dye Decolorization
Authors: Peláez Rubén Darío Romero , Serra Luana Assis , Wischral Daiana , Cunha Joice Raísa Barbosa , Mendes Thais Demarchi , Pacheco Thályta Fraga , de Siqueira Felix Gonçalves , de Almeida João Ricardo Moreira
Published: October 2023

This work investigates the effects of the co-culture between the filamentous fungus Panus lecomtei and the yeast Sporidiobolus pararoseus in the production of laccases. The variations of time interval and inoculum volume of S. pararoseus in co-cultures with P. lecomtei stimulated laccase production, reaching its highest activity at nearly 2960.7 ± 244 U/mL with a maximum time point of 120 h and 2.0% (v/v), respectively. Further application in the pretreated sugarcane bagasse hydrolysis was performed, using P. lecomtei and S. pararoseus extract added to an enzyme mixture from the co-culture of P. lecomtei and Trichoderma reesei that positively favored the hydrolysis efficiency by 66.87%. Furthermore, the addition of P. lecomtei and S. pararoseus extract increased the degradation of industrial anthraquinone Remazol Brilliant Blue R by 78.98%. As a result, the extract derived from the co-culture of P. lecomtei and S. pararoseus rich in laccases presents potential in biotechnological applications, being suitable in the hydrolysis of lignocellulosic biomass and the degradation of unwanted dyes released in the environment.
How Many Hungarian Consumers Choose Lactose- and Gluten-Free Food Products Even When They Do Not Necessarily Need to?
Authors: Kasza Gyula , Szabó Erika , Izsó Tekla , Ózsvári László
Published: October 2023

The popularity of “free-from” food products (FFFPs), which exclude several ingredients such as lactose, gluten, or sugar, is increasing globally. However, experts agree that avoiding these ingredients without medical reasons can lead to nutritional deficiencies. A representative consumer survey was conducted in Hungary (n = 1002); it focused on behaviors related to FFFPs, particularly lactose- and gluten-free products. This study revealed that consumers often consider “free-from” claims during shopping. Lactose- and gluten-free foods were popular, even among those without specific dietary needs. A distinct “free-from consumer group” (7.8% of the sample, predominantly women) was identified, who consume both lactose- and gluten-free foods frequently. However, only 15.4% of the group had medical reasons for their preference, such as lactose intolerance or gluten sensitivity. The majority (75.6%) chose these products without medical justification, relying on self-diagnosis, through the involvement of family members, or the belief that they were healthier. This consumer group accounts for nearly 6% of Hungary’s adult population, exceeding 470,000 individuals. Extrapolating these figures to other European countries suggests that 25–30 million EU citizens might be in a similar situation, highlighting the need for improved health education and awareness-raising campaigns to prevent imbalanced nutrition and foster the recognition and treatment of real health problems.
Utility of the Comprehensive Trail Making Test in the Assessment of Mild Cognitive Impairment in Older Patients
Authors: Bednorz Adam , Religa Dorota
Published: October 2023

Introduction. The purpose of this study is to determine the usefulness of the CTMT (Comprehensive Trail Making Test) in diagnosing mild cognitive impairment in older patients. The test is used to assess executive functions, of which impairment is already observed in the early stages of the neurodegenerative process. Materials and Methods. The study includes 98 patients of a geriatric ward assigned to 2 groups of 49 patients each: patients diagnosed with a mild cognitive impairment and patients without a cognitive impairment, constituting the control group (group K). A set of screening tests was used in the initial study: the MMSE (Mini-Mental State Examination), MoCA (Montreal Cognitive Assessment), and CDT (Clock Drawing Test), GDS (Geriatric Depression Scale). The second study included the performance of the CTMT; the performance indicator was the time of performance. Results. Statistically significant differences are obtained between patients with mild cognitive impairments and those in cognitive normality in the performance of the CTMT test (p < 0.01). Patients with MCIs took longer to complete all trails of the test. To identify cognitive impairment, cutoff points were proposed for the CTMT total score and the other test trails. The CTMT overall score and CTMT 5 scored the highest AUCs (CTMT overall score = 0.77, CTMT Trail 5 = 0.80). Conclusions. The Comprehensive Trail Making Test may be useful in diagnosing mild cognitive impairment as a complementary screening tool.
Establishing Age-Based Color Changes for the American Burying Beetle, Nicrophorus americanus Olivier, with Implications for Conservation Efforts
Authors: McMurry Robert Shane , Cavallaro Michael C. , Shufran Andrine , Hoback William Wyatt
Published: October 2023

The American burying beetle, Nicrophorus americanus Olivier, is a federally protected insect that once occupied most of eastern North America. Adult beetles feature distinct, recognizable markings on the pronotum and elytra, and color changes with age have been observed. Among the challenges faced by research scientists and conservation practitioners is the ability to determine beetle age in the field between and including teneral (young) and senescent (old) adult stages. Using 20 (10 male and 10 female) captive-bred beetles, we characterized the change in greyscale and red, green, and blue (RGB) color channels over the lifespan of each beetle for field-aging applications. Individual beetles were photographed at set intervals from eclosion to death, and color data were extracted using open-source ImageJ Version 1.54f software. A series of linear mixed-effects models determined that red color showed the steepest decrease among all color channels in the pronotum and elytral markings, with a more significant decrease in the pronotum. The change in greyscale between the pronotum and elytral markings was visibly different, with more rapid darkening in the pronotum. The resulting pronotum color chart was tested under field conditions in Oklahoma, aging 299 adult N. americanus, and six age categories (day range) were discernable by eye: teneral (0–15), late teneral (15–31), early mature (31–45), mature (45–59), early senescent (59–76), and senescent (76–90). The ability to more precisely estimate age will improve population structure estimates, laboratory breeding programs, and potential reintroduction efforts.
The Impact of Climate on the Energetics of Overwintering Paper Wasp Gynes (Polistes dominula and Polistes gallicus)
Authors: Kovac Helmut , Käfer Helmut , Petrocelli Iacopo , Amstrup Astrid B. , Stabentheiner Anton
Published: October 2023

Gynes of paper wasps (Polistes sp.) spend the cold season in sheltered hibernacles. These hibernacles protect against predators and adverse weather conditions but offer only limited protection against low temperatures. During overwintering diapause, wasps live on the energy they store. We investigated the hibernacles’ microclimate conditions of species from the Mediterranean (Italy, P. dominula, P. gallicus) and temperate (Austria, P. dominula) climates in order to describe the environmental conditions and calculate the energetic demand of overwintering according to standard metabolic rate functions. The temperatures at the hibernacles differed significantly between the Mediterranean and temperate habitats (average in Austria: 3.2 ± 5.71 °C, in Italy: 8.5 ± 5.29 °C). In both habitats, the hibernacle temperatures showed variance, but the mean hibernacle temperature corresponded closely to the meteorological climate data. Cumulative mass-specific energetic costs over the studied period were the lowest for the temperate P. dominula population compared with both Mediterranean species. The lower costs of the temperate species were a result of the lower hibernacle temperature and acclimation to lower environmental temperatures. Model calculations with an increased mean temperature of up to 3 °C due to climate change indicate a dramatic increase of up to 40% in additional costs.
Cardiovascular Toxicities of Radiation Therapy and Recommended Screening and Surveillance
Authors: Narowska Gabriela , Gandhi Sakshi , Tzeng Allison , Hamad Eman A.
Published: October 2023

Radiation therapy is a key part of treatment for many cancers. Vast advancements in the field of radiation oncology have led to a decrease in malignancy-related mortality, which has uncovered some of the long-term side effects of radiation therapy. Specifically, there has been an increase in research looking into the cardiovascular side effects of chest radiation therapy for cancers of the esophagus, breast, and lung tissue as well as lymphomas. The manifestations of cardiac injury from irradiation range from short-term complications, such as pericarditis, to long-term damage including cardiomyopathy, valvular disease, and conduction disturbances. The aims of this article are to describe the cardiovascular side effects and the associated risk factors, to discuss risk reduction strategies, and to provide guidance in pre-radiation screening, post-radiation surveillance, and the management of these conditions.
Contaminant Metals and Cardiovascular Health
Authors: Lundin Karl Kristian , Qadeer Yusuf Kamran , Wang Zhen , Virani Salim , Leischik Roman , Lavie Carl J. , Strauss Markus , Krittanawong Chayakrit
Published: October 2023

A growing body of research has begun to link exposure to environmental contaminants, such as heavy metals, with a variety of negative health outcomes. In this paper, we sought to review the current research describing the impact of certain common contaminant metals on cardiovascular (CV) health. We reviewed ten metals: lead, barium, nickel, chromium, cadmium, arsenic, mercury, selenium, zinc, and copper. After a literature review, we briefly summarized the routes of environmental exposure, pathophysiological mechanisms, CV health impacts, and exposure prevention and/or mitigation strategies for each metal. The resulting article discloses a broad spectrum of pathological significance, from relatively benign substances with little to no described effects on CV health, such as chromium and selenium, to substances with a wide-ranging and relatively severe spectrum of CV pathologies, such as arsenic, cadmium, and lead. It is our hope that this article will provide clinicians with a practical overview of the impact of these common environmental contaminants on CV health as well as highlight areas that require further investigation to better understand how these metals impact the incidence and progression of CV diseases.
Gothic Fairy-Tale Feminism: The Rise of Eyre/‘Error’
Authors: Farrar Aileen Miyuki
Published: October 2023

The ways Gothic fairy tales and fairy-tale feminism interact are not always clear. An undercurrent of feminist studies of fairy tales is fueled by the 1970s Lurie-Lieberman debate, which focused on the question of whether fairy tales liberate or repress women. Meanwhile, critics such as Lorna Piatti-Farnell and Lucie Armitt have offered studies of the interplay between Gothic horror and fairy tales. However, these studies have limits, often emphasizing the violence, self-mutilation, and cannibalism of women, like those in Jacob and Wilhelm Grimm’s versions of “Cinderella” and “Snow White”. This paper argues that “Rapunzel” (1812) is key for understanding the Gothic and feminist discourses of Charlotte Brontë’s Jane Eyre (1847). Firstly, this paper argues that a self-reflexive and self-productive relationship between subjectivity and desire shapes and disrupts the Gothic, fairy-tale, and feminist discourses of Jane Eyre, resulting in a specular feminine-I that has inspired pluralistic readings of the text. Secondly, an analysis of the Rapunzelian metaphors of ‘wicked’ hunger and ideological towers unmasks the double consciousness that not only fetters feminine subjectivity but delimits the domestic structures of marriage and home. Multiplying the ways nineteenth-century Gothicism, fairy tales, and feminism may interact, Brontë’s specular study of feminine desire makes way for a productive and agential feminine speaking-I.
A Novel Spacetime Boundary-Type Meshless Method for Estimating Aquifer Hydraulic Properties Using Pumping Tests
Authors: Ku Cheng-Yu , Liu Chih-Yu
Published: October 2023

This article introduces a new boundary-type meshless method designed for solving axisymmetric transient groundwater flow problems, specifically for aquifer tests and estimating hydraulic properties. The method approximates solutions for axisymmetric transient groundwater flow using basis functions that satisfy the governing equation by solving the inverse boundary value problem in the spacetime domain. The effectiveness of this method was demonstrated through validation with the Theis solution, which involves transient flow to a well in an infinite confined aquifer. The study included numerical examples that predicted drawdown at various radial distances and times near pumping wells. Additionally, an iterative scheme, namely, the fictitious time integration method, was employed to iteratively determine the hydraulic properties during the pumping test. The results indicate that this approach yielded highly accurate solutions without relying on the conventional time-marching scheme. Due to its temporal and spatial discretization within the spacetime domain, this method was found to be advantageous for estimating crucial hydraulic properties, such as the transmissivity and storativity of an aquifer.
Asymmetric Graph Contrastive Learning
Authors: Chang Xinglong , Wang Jianrong , Guo Rui , Wang Yingkui , Li Weihao
Published: October 2023

Learning effective graph representations in an unsupervised manner is a popular research topic in graph data analysis. Recently, contrastive learning has shown its success in unsupervised graph representation learning. However, how to avoid collapsing solutions for contrastive learning methods remains a critical challenge. In this paper, a simple method is proposed to solve this problem for graph representation learning, which is different from existing commonly used techniques (such as negative samples or predictor network). The proposed model mainly relies on an asymmetric design that consists of two graph neural networks (GNNs) with unequal depth layers to learn node representations from two augmented views and defines contrastive loss only based on positive sample pairs. The simple method has lower computational and memory complexity than existing methods. Furthermore, a theoretical analysis proves that the asymmetric design avoids collapsing solutions when training together with a stop-gradient operation. Our method is compared to nine state-of-the-art methods on six real-world datasets to demonstrate its validity and superiority. The ablation experiments further validated the essential role of the asymmetric architecture.
The Efficient Energy Collection of an Autoregulatory Driving Arm Harvester in a Breeze Environment
Authors: Zhang Chao , Yang Xinlong , Zhang Boren , Fan Kangqi , Liu Zhiming , Liu Zejia
Published: October 2023

Breezes are a common source of renewable energy in the natural world. However, effectively harnessing breeze energy is challenging with conventional wind generators. These generators have a relatively high start-up wind speed requirement due to their large and steady rotational inertia. This study puts forth the idea of an autoregulatory driving arm (ADA), utilizing a stretchable arm for every wind cup and an elastic thread to provide adjustable rotational inertia and a low start-up speed. The self-adjustable rotational inertia of the harvester is achieved through coordinated interaction between the centrifugal and elastic forces. As the wind speed varies, the arm length of the wind cup automatically adjusts, thereby altering the rotational inertia of the harvester. This self-adjustment mechanism allows the harvester to optimize its performance and adapt to different wind conditions. By implementing the suggested ADA harvester, a low start-up speed of 1 m/s is achieved due to the small rotational inertia in its idle state. With the escalation of wind speed, the amplified centrifugal force leads to the elongation of the driving arms. When compared to a comparable harvester with a constant driving arm (CDA), the ADA harvester can generate more power thanks to this stretching effect. Additionally, the ADA harvester can operate for a longer time than the CDA harvester even after the wind has stopped. This extended operation time enables the ADA harvester to serve as a renewable power source for sensors and other devices in natural breeze environments. By efficiently utilizing and storing energy, the ADA harvester ensures a continuous and reliable power supply in such settings.
Comparison of PDMS and NOA Microfluidic Chips: Deformation, Roughness, Hydrophilicity and Flow Performance
Authors: Turcitu Tatiana , Armstrong Curtis J. K. , Lee-Yow Niko , Salame Maya , Le Andy Vinh , Fenech Marianne
Published: October 2023

Microfluidic devices are frequently manufactured with polydimethylsiloxane (PDMS) due to its affordability, transparency, and simplicity. However, high-pressure flow through PDMS microfluidic channels lead to an increase in channel size due to the compliance of the material. As a result, longer response times are required to reach steady flow rates, which increases the overall time required to complete experiments when using a syringe pump. Due to its excellent optical properties and increased rigidity, Norland Optical Adhesive (NOA) has been proposed as a promising material candidate for microfluidic fabrication. This study compares the compliance and deformation properties of three different characteristic sized (width of parallel channels: 100, 40 and 20 µm) microfluidic devices made of PDMS and NOA. The comparison of the microfluidics devices is made based on the Young’s modulus, roughness, contact angle, channel width deformation, flow resistance and compliance. The experimental resistance is estimated through the measurement of the flow at a given pressure and a precision flow meter. The characteristic time of the system is extracted by fitting the two-element resistance-compliance (RC) hydraulic circuit model. The compliance of the microfluidics chips is estimated through the measurement of the characteristic time required for channels to achieve an output flow rate equivalent to that of the input flow rate using a syringe pump and a precision flow meter. The Young modulus was found to be 2 MPa for the PDMS and 1743 MPa for the NOA 63. The surface roughness was found to be higher for the NOA 63 than for the PDMS. The hydrophilicities of materials were found comparable with and without plasma treatment. The results show that NOA devices have lower compliance and deformation than PDMS devices.
Anchor Loss Reduction in Micro-Electro Mechanical Systems Flexural Beam Resonators Using Trench Hole Array Reflectors
Authors: Kazemi Mohammad , Nabavi Seyedfakhreddin , Gratuze Mathieu , Nabki Frederic
Published: October 2023

The quality factor of microelectromechanical resonators is a crucial performance metric and has thus been the subject of numerous studies aimed at maximizing its value by minimizing the anchor loss. This work presents a study on the effect of elastic wave reflectors on the quality factor of MEMS clamped–clamped flexural beam resonators. The elastic wave reflectors are a series of holes created by trenches in the silicon substrate of the resonators. In this regard, four different shapes of arrayed holes are considered, i.e., two sizes of squares and two half circles with different directions are positioned in proximity to the anchors. The impact of these shapes on the quality factor is examined through both numerical simulations and experimental analysis. A 2D in-plane wave propagation model with a low-reflecting fixed boundary condition was used in the numerical simulation to predict the behavior, and the MEMS resonator prototypes were fabricated using a commercially available micro-fabrication process to validate the findings. Notably, the research identifies that half-circle-shaped holes with their curved sides facing the anchors yield the most promising results. With these reflectors, the quality factor of the resonator is increased by a factor of 1.70× in air or 1.72× in vacuum.