bluuebunny/crossref_metadata_2025 huggingface dataset:
Chromomycosis: literature review and clinical case
Authors: Yakovlev A. B.
Published: November 2021

A clinical case of leg skin chromomycosis is described, a review of the literature is given, the main modern medical methods, as well as methods of historical significance are briefly described. The individual links of the pathogenesis of chromomycosis (CM) are described, a list of nosologies to which chromomycosis should be differentiated is presented.

Materials and methods. A clinical case of chromomycosis in a 63-year-old patient is presented. The process developed as a result of systematic frequent injury of the shins with sharp parts of plants.

Results. The diagnosis of chromomycosis was established on the basis of anamnesis, clinic data, detection of the pathogen in the biopsy material.

Conclusions. Thus, CM is a serious deep skin mycosis, which still causes an epidemiologically significant morbidity in workers of agricultural and related fields! It is difficult to treat it, especially in more severe clinical forms. Treatment usually consists of long periods of treatment with antifungal drugs, often associated with physical procedures and immunotherapy. This publication is intended to extract HMM from the list of ′forgotten diseases′ at least for a short time!
Efficacy of interleukin 17 inhibitor therapy in overweight and obese psoriasis patients (preliminary data)
Authors: Vladimirova I. S. , Smirnova I. O. , Obrezan A. G. , Zhelonkin A. R. , Yantsevich N. K.
Published: November 2021

In recent years, comorbidity in psoriasis has been actively studied, one of the most significant of which is obesity. Obesity is a risk factor for dermatosis in susceptible individuals, which can affect the effectiveness of therapy for the disease, including genetically engineered biological drugs.

The aim of the study. To study the effectiveness of therapy with an interleukin‑17 inhibitor (iskekizumab) and the dynamics of lipid parameters in patients with psoriasis and overweight and obesity.

Material and methods. A retrospective study of 25 patients diagnosed with psoriasis vulgaris was carried out. Inclusion criteria were PASI more than 12 points, BSA more than 10% and sPGA more than 3 points, age over 18 years. Anthropomeric parameters and lipid profile data were studied. All patients received iksekizumab treatment according to the standard regimen. Evaluation of the effectiveness of therapy was carried out according to the dynamics of PASI indicators, as well as the frequency of patients achieving the response PASI 75, PASI 90 and PASI 100.

Results. Obesity and overweight were diagnosed in 13 patients who made up the main observation group. The comparison group included 12 nonobese patients. Patients in the observation groups did not differ in sex and severity of psoriasis. In patients of the main group, hypertriglyceridemia was significantly more often diagnosed (55.6%; 95% CI: 33.7–75.4; in its absence in the comparison group; p = 0.0200), as well as other comorbidity – hypertension and metabolic syndrome (p = 0.0016 and p = 0.0052, respectively). On the background of therapy, skin rashes were resolved in patients of both observation groups. By the seventh week of therapy, there was a significant decrease in PASI, sPGA and BSA, by the 36th week, the rash was completely resolved in all patients (p < 0.001). There were no significant differences between the groups in the dynamics of clinical indicators of the severity of the disease. Body mass index did not change statistically significantly over the observation period in patients of both groups (p = 0.6690). Changes in lipid profile for all parameters were statistically insignificant. There were no significant differences in the frequency of achieving PASI of 75, 90 and 100% between the groups.
Antipruritic therapy issues on background of skin xerosis
Authors: Kruglova L. S. , Tipaeva E. V.
Published: November 2021

The relationship of xerosis with various skin diseases is very multifaceted, at the same time, xerosis can be the cause of the onset or aggravation of itching. Modern recommendations for the external treatment of chronic dermatoses, accompanied by xerosis and itching, imply two directions: direct therapeutic measures during an exacerbation and the use of dermatocosmetics adapted to certain symptoms.

Material and methods. We observed 26 patients (mean age 38.7 ± 1.9 years with atopic dermatitis, eczema, psoriasis or pruritus, where xerosis was present in the clinical picture and pruritus was noted. All patients used topical drugs in accordance with the nosology, in as an adjuvant therapy – Neotanin Comfort Plus cream.

Research results. In atopic dermatitis, the corneometry index increased by an average of 21%, with eczema – by 20%, with psoriasis – by 22%, with skin itching – by 12%. Transepidermal water loss decreased in atopic dermatitis by 19.8%, with eczema – by 22.8%, with psoriasis – by 21.8%, with pruritus – by 18.4% The value of the total BRS index decreased by more than two times.

Conclusion. The use of Neotanin Comfort Plus cream in combination with topical drug therapy (GCS, multicomponent drugs) is highly effective and safe in patients with atopic dermatitis, eczema, psoriasis or pruritus, which makes it possible to recommend it for use in wide clinical practice.
Case of bullous pemphigoid in patient with psoriasis, after new coronavirus infection COVID‑19
Authors: Rudneva N. S. , Sadunashvili T. G. , Chumakova Ya. Yu. , Natarova E. V. , Losev A. V.
Published: November 2021

In this work we discuss a case of Bullosus pemfigoid (B.P.) developed after pneumonia caused by new coronavirus disease COVID‑19 in a patient with psoriasis. Provided description is supported with the data of histological and serologic analysis, the lgG of epithelial basement membrane was detected to antigen ВР230-CF and to antigen ВР180-NC16A.
Experience of using photodynamic therapy for treatment of genitourinary syndrome of menopause
Authors: Surkichin S. I. , Avin M. , Mayorov R. Yu.
Published: November 2021

This study reports about efficacy and safety of photodynamic therapy in Genitourinary Syndrome of Menopause (GSM).

Material and methods. The study group included 12 patients with a diagnosis of urogenital syndrome of menopause. The trismeglumine salt of chlorin E6 in the form of a 1% gel was used as a photosensitizer. LED phototherapy apparatus Garmonia (photodynamic therapy apparatus (emitter 660 nm) was used as a radiation source. The PDT procedure was performed once every 7 days. The following scales were used to assess the effectiveness: Vaginal Health Index, Visual Analogue Scale, Dermatology Life Quality Index score, and general satisfaction with the course of procedures was also assessed.

Results. The patients noted a significant regression of itching, burning, pain and dyspareunia after the course of photodynamic therapy. There was a decrease in dryness, on average, up to 3 points upon completing the course of procedures. Itching completely regressed in 6 out of patients. Dyspareunia significantly decreased in 4 patients and completely disappeared in 2 patients. The greatest effect according to the results of assessing the index of vaginal health was noted in relation to Ph-metry, as well as in relation to elasticity (4.17 points) and moisture (4.33 points). The median satisfaction score with treatment outcomes was 2.7 at last follow-up. There were no serious side effects during the entire period of treatment and after it.

Conclusions. The following study reports that the photosensitizer with trismeglumine salt of chlorin E6 and PDT with a wavelength of 660 nm with an exposure for 30 minutes, an energy of 100 J/cm2, a power of 100 mV/cm2 , once every 7 days, is an effective and harmless method of treating GSM.
ST. JOHN'S WORT (HYPERICUM PERFORATUM L.) FLOWER BASED CARBON/GRAPHENE QUANTUM DOT STRUCTURE PRODUCTION AND CHARACTERIZATION FOR BIOIMAGING AND DRUG DELIVERY SYSTEMS
Authors: KARACA AÇARI İdil , ÖNAL Yunus
Published: November 2021

In this study, it was aimed to obtain carbon and graphene quantum dot structures from St. John's Wort (Hypericum perforatum L.) flowers, originating from the city of Hatay. Hypericum perforatum L. flower sample was subjected to carbonization at different temperatures such as 200, 225 and 250 ℃ for the desired quantum dot structure yields. It has been observed that the best radiation after carbonization is at 250 ℃. Fourier transform infrared spectrometer, X-ray diffraction and scanning electron microscopy techniques were used to determine the structural characterizations and surface morphology, respectively. The UV radiation of Hypericum perforatum L. flower-based carbon and graphene quantum structures was followed at 365 nm and the blue glow was observed very clearly. With this study, quantum and graphene dot structures based on Hypericum perforatum L. flower have been introduced to the literature for the first time. In addition, the quantum dot structures with blue radiation obtained within the scope of the study will be an alternative reference for many bioimaging and drug delivery system studies.
Blokzincir Uygulamalarında Kişisel Veri Problemi: Depolama Riskleri ve Öneriler
Authors: DİRİ Nurcan , YALÇINKAYA Bahattin
Published: November 2021

Bilgi ve İletişim Teknolojilerinin (BİT) hızla gelişmesiyle birlikte, çok miktarda kişisel veri oluşmakta, kullanılmakta ve depolanmaktadır. Depolanmakta olan kişisel veriler, son kullanıcıların teknik ve hukuki yönlerden korunmalarını gerektirmektedir. Blokzincir teknolojisi kişisel verilerin gizliliğini korumak ve kontrolünü sağlamak için son yıllarda önemli gelişmeler kaydeden yenilikçi teknoloji olarak görülmektedir. Merkezi olmayan ve Eşler Arası (Peer-to-Peer-P2P) bir dağıtık dijital defter olan blokzincir teknolojisi, dijital varlıkların tüm işlemlerini depolayabilen, merkezi olmayan, doğrulanabilir ve değiştirilemez bir defter hizmeti sunar. Bir ağdaki katılımcılarla onlara tam olarak güvenmeye gerek kalmadan veri paylaşma konusunda yeni bir yaklaşım sunmaktadır. Yakın zamanda tanıtılan Genel Veri Koruma Yönetmeliği (GDPR) ve Kişisel Verilerin Korunması Kanunu (KVKK), kişisel verilerin nasıl ele alınacağı konusunda büyük değişiklikler getirmektedir. GDPR ve KVKK, kişisel verilerin kullanmasıyla veri denetleyicileri ve işlemciler için yeni koruma zorunlulukları getirmiştir. GDPR ve KVKK, veri koruma mevzuatı kapsamında birliğinin sağlanması için kişisel olarak tanımlanan verilere (PII) daha kolay erişim, silme, düzeltme ve taşıma hakkı verilmesi gibi yeni uygulamalar getirmektedir. GDPR ve KVKK kapsamında, merkezi yapıların hâkim olduğu bir toplumda kişisel veri işleme faaliyetlerinin çoğunlukla merkezi yapılar tarafından gerçekleştirilmesi ve uyulması gereken bir takım usul ve esasları vardır. Ancak blokzincir platformunda ortaya koyulan araştırmalarda kişisel olarak tanımlanan verilerin saklanmasında; merkezi tüzel veya gerçek kişilerin veri saklama, işleme ve silme, gibi uygulamaları gerçekleştirmesi yönünde hazırlanan KVKK ve GDPR hükümlerinin uygulanmasında bazı uyumsuzluklar bulunmaktadır. Bu çalışmada, blokzincirin temel özellikleri detaylandırılmış, kişisel verilerinin kullanımı için blokzincir teknolojisi destekli çözümler açıklanmış ve konuya dair sorunlar ile zorlukları tartışılmıştır. Literatür incelendiğinde; kişisel verilerin günümüzde blokzincir ağında saklanmamasına yönelik tavsiyeler verildiği görülmüştür. Kişisel verilerin KVKK ve GDPR kapsamındaki birincil haklarının, blokzincir teknolojisinin karakteristik yapısına uygun olmadığı, akademik ve uygulamalı araştırmalarda gösterilmiştir. Blokzincir teknolojisindeki gelişme ve güncellemelerin, teknolojinin kendisi ile çelişeceği ve blokzincirin karakteristik özelliğini yok edeceği akademik çevrelerce düşünülmekte ve bundan dolayı temel yapıyı etkilemeyecek (özel anahtarın silinmesi, zincir dışı depolama, karma değeri silinmesi vb.) küçük çaplı değişikliklerin yapılması önerilmektedir.
Perbedaan Pemahaman Matematis Ditinjau Gaya Kognitif dan Gender Siswa Kelas VII SMPN 12 Kendari
Authors: Ayu Nita Aprilia , Fahinu Fahinu , Busnawir Busnawir
Published: November 2021

Penelitian ini bertujuan untuk: (1) Untuk mengetahui gaya kognitif siswa SMP Negeri 12 Kendari, (2) Untuk mengetahui pemahaman matematis siswa SMP Negeri 12 Kendari, (3) Untuk mengetahui perbedaan pemahaman matematis siswa ditinjau dari gaya kognitif field dependent dan gaya kognitif filed independent, (4) Untuk mengetahui perbedaan pemahaman matematis siswa ditinjau dari gender dan (5) Untuk mengetahui perbedaan pemahaman matematis siswa ditinjau dari gaya kognitif dan gender. Jenis penelitian ini adalah penelitian ex post facto.Teknik pengumpulan data menggunakan tes pemahaman matematis dan tes GEFT. Hasil penelitian sebagai berikut: (1) Terdapat perbedaan pemahaman matematis siswa ditinjau dari gaya kognitif field dependent dan gaya kognitif filed independent, (2) Tidak ada perbedaan pemahaman matematis siswa ditinjau dari gender. (3) Tidak ada perbedaan pemahaman matematis siswa ditinjau dari gaya kognitif dan gender.
Analisis Fitokimia dan Aktivitas Antibakteri Beberapa Jenis Tanaman terhadap Bakteri Patogen pada Budidaya Ikan
Authors: Salosso Yuliana , Olga Olga , Aisiah Siti , Ressie Jeny Dorotea , Foes Yenni Welhelmina , Pasaribu Wesley
Published: November 2021

Penelitian ini bertujuan untuk mengetahui kandungan fitokimia serta aktivitas antibakteri sepuluh jenis tanaman  terhadap bakteri Vibrio alginolyticus dan Aeromonas hydropilla. Dipilih  10 jenis tanaman yang sering digunakan sebagai obat dan banyak ditemukan di kota Kupang seperti tanaman Phyllanthus acidus, Euphorbia hirta, Persea gratissima, Euphorbia thymifolia,  Morus australis, Justica gendarussa, Paederia scandeus, Annona squamosa,  Sesbania sesban,  dan Jatropha gossypifolia. Bagian tanaman yang digunakan adalah daun, kecuali E. hirta dan E. thymifolia digunakan seluruh bagian tanaman.  Kesepuluh jenis tanaman tersebut dibuat bubuk,  kemudian diekstraksi dengan cara dimaserasi secara bertingkat mulai dari pelarut n-heksan, etil asetat dan metanol, selain itu, juga digunakan ekstrak air  tanaman tersebut. Semua jenis ekstrak tanaman diuji antibakteri dengan menggunakan metode cakram. Jenis ekstrak yang aktif selanjutnya diuji antibakteri pada konsentrasi 10%, 1% dan 0,1%.  Hasil penelitian menunjukkan bahwa jenis  tanaman  yang berpotensi sebagai antibakteri pada budidaya ikan laut adalah  esktrak air dan metanol E. hirta dan E. thymifolia. Sedangkan jenis tanaman yang berpotensi sebagai antibakteri pada budidaya ikan air tawar adalah ekstrak air  E. hirta dan E. thymifolia daun J. gendarussa.
PENEMPATAN SDM POLRI MENUJU PERSONIL YANG PROFESIONAL PADA KEPOLISIAN DAERAH PROVINSI SULAWESI TENGGARA
Authors: Amrullah Amrullah , Suaib Eka , Yusuf Muhammad
Published: November 2021

Penelitian ini bertujuan menganalisis penempatan sumber daya manusia Polri, menganalisis profesionalisme personil Polri dan mendeskripsikan faktor-faktor yang mempengaruhi penempatan sumber daya manusia Polri pada Kepolisian Daerah Provinsi Sulawesi Tenggara,  Penelitian ini menggunakan pendekatan deskriptif  kualitatif  menggunakan teknik analisis model interaktif terdiri dari pengumpulan data, reduksi data, penyajian data dan penarikan kesimpulan/verivikasi, Hasil penelitian menunjukan penempatan sumber daya manusia Polri secara umum masih belum maksimal dan belum konsisten menerapkan prinsip meryt system dalam penyelenggaraan promosi, mutasi dan demosi personel, secara prosedural penempatan personel telah dilaksanakan dengan memperhatikan segala ketentuan pedoman/petunjuk yang tertuang PerkaPolri, namun masih ada tendensi terjadinya tindakan afirmatif yang berkembang dalam pelaksanaanya. Profesionalisme personil Polri dapat dikatakan cukup baik, anggota personel telah mempunyai keterampilan  tinggi dalam suatu bidang ,mempunyai ilmu &  pengetahuan  sesuai dengan  pendidikan  & pelatihan kejuruan mencakup keahlian teknik, taktik, strategi, maupun manajemen yang didukung dengan pertanggung jawaban administrasi sesuai dengan jenis bentuk dan tatarannya, mempunyai sikap yang berorientasi ke depan dengan  melakukan pemantapan terhadap fungsi mitigasi dan penegakan hukum menyikapi empat jenis kejahatan (konvensional, transnasional,kekayaan negara, dan kejahatan berimplikasi kontijensi) , telah mempunyai sikap mandiri dengan cukup baik dalam  melakasanakan tugas & fungsi kepolisian baik fungsi utama, fungsi operasional, fungsi organik, dan fungsi pendukung Polri lainya. Kata Kunci: Penempatan SDM, Profesional Polri, Polda Sultra
ANALISIS PERAN STAKEHOLDER DALAM PERENCANAAN PEMBANGUNAN DAERAH KABUPATEN BUTON UTARA
Authors: Amalan Amalan , Utha Arifin , Yusuf Muhammad
Published: November 2021

Tujuan penelitian ini menganalisis peran stakeholder dalam perencanaan pembangunan daerah di Kabupaten Buton Utara. Penelitian kualitatif ini menggunakan studi kasus deskriptif. Informan penelitian berjumlah sembilan orang yang ditentukan secara purposif. Teknik pengumpulan data yang digunakan adalah observasi, wawancara dan dokumentasi. Analisis data menggunakan model deskripsi kualitatif. Hasil penelitian menunjukkan peran stakeholder dalam perencanaan pembangunan daerah selaku pembuat kebijakan, fasilitator, koordinator, pelaksana dan mempercepat proses perencanaan belum berjalan secara optimal di Kabupaten Buton Utara. Sebagai pembuat kebijakan yakni ikut membahas program pembangunan yang ditetapkan dalam bentuk RPJMD, RKPD dan Renstra masih terdapat ketidaksinkronan. Musrenbang hanyalah sebatas partisipasi ide, gagasan, usulan dan penyampaian masukan saja. Koordinasi dan fasilitasi yang diperankan oleh Bappeda Kabupaten Buton Utara belum berjalan dengan baik, demikian pula SKPD dalam mengimplementasikan pelaksanaan kebijakan juga masih terjadi ketidakselarasan. Belum nampak peran stakeholders yang memberikan kontribusi dalam mempercepat capaian target sasaran pembangunan. Kondisi ini dapat mengakibatkan target yang direncanakan belum tercapai secara keseluruhan. Kata kunci: perencanaan pembangunan, program pembangunan, stakeholders
PENGEMBANGAN KEMAMPUAN SUMBERDAYA APARATUR DI SEKRETARIAT DAERAH KABUPATEN KOLAKA
Authors: Suryandi Endang , Suaib Eka , Utha Arifin
Published: November 2021

Penelitian ini bertujuan untuk menganalisis Pelaksanaan Pengembangan Kemampuan Sumberdaya Aparatur Pegawai Negeri Sipil di Sekretariat Daerah Kabupaten Kolaka. Penelitian ini menggunakan pendekatan kualitatif dengan informan sebanyak 9 orang yang ditentukan secara purposive sampling. Data dalam penelitian ini diperoleh melalui wawancara dan studi dokumentasi, data yang diperoleh ini kemudian analisis secara kualitatif melalui analisis data interaktif yang terdiri dari reduksi data, penyajian data serta penarikan kesimpulan. Hasil penelitian ini menunjukkan bahwa Pelaksanaan pengembangan Sumberdaya  Aparatur di Sekretariat Daerah Kabupaten Kolaka, baik melalui pendidikan formal maupun non formal, perlu mendapat perhatian yang serius dari Pemerintah Daerah Kabupaten Kolaka, khususnya dukungan dari pimpinan Sekretariat Daerah Kabupaten Kolaka untuk melakukan diplomasi terutama terkait dengan kuota peserta tugas belajar, dan diklat serta peningkatan anggaran untuk kegiatan pengembangan kemampuan sumberdaya aparatur  di Sekretariat Daerah Kabupaten Kolaka.Kata Kunci: Pengembangan Kemampuan, Sumberdaya Aparatur Sipil Negara, Pendidikan Latihan
Communicating climate risk: a handbook
Authors: Roberts Freya Eloise , De Meyer Kris , Hubble-Rose Lucy
Published: November 2021

This handbook is a practical guide to communicating climate risk, designed specifically for those working at the interface of climate science and policy. It explains insights from psychology and neuroscience on how our brains engage with the idea of climate risk, it highlights journalism hacks for writing about risk clearly, it shares lessons learned from the authors' experience working with policymakers on climate risk, and it offers a set of useful questions to help other researchers ascertain what policymakers need from climate risk research. Contact the authors on Twitter @UCL_CAU or email climateactionunit@ucl.ac.uk for further information.
Gambaran Kelelahan Kerja Karyawan PT Paving Meriba Jaya Kelurahan Tavanjuka Kota Palu
Authors: Politon Fellysca Veronica Margareth , Christine Christine
Published: November 2021

Kelelahan kerja merupakan salah satu permasalahan keselamatan dan kesehatan kerja yang dapat menjadi faktor risiko terjadinya kecelakaan kerja dan dapat berdampak terhadap penurunan produktivitas kerja dan penurunan konsentrasi kerja. Di Kota Palu terdapat tiga Pabrik Paving Yaitu PT. Supplier Paving Batako Press Kanstin, PT. Watu Meriba Jaya, dan PT. Batu Split Palu. PT. Watu Meriba Jaya memiliki jumlah pekerja dengan jumlah total pekerja 38 orang yang terbagi pada divisi pencampuran, pencetakan, pengeringan, inising (penghalusan produk), serta pembuatan behel dan driver. Tujuan penelitian ini adalah diketahuinya Gambaran kelelahan kerja karyawan di PT. Watu Meriba Jaya Kelurahan Tavanjuka Kota Palu. Metode dalam penelitian ini adalah deskriptif dengan. Sampel penelitian ini adalah 38 karyawan. Data diolah dengan Analisa Univariate untuk menampilkan gambaran kelelahan kerja karyawan PT.Watu Meriba Jaya Tavanjuka Kota palu dan disajikan dalam bentuk tabel dan narasi. Hasil penelitian ini menunjukan bahwa masa kerja terbanyak adalah masa kerja baru 32 orang (84,2%). status gizi terbanyak adalah status gizi normal 33 orang (86,8%) . Divisi kerja terbanyak yaitu percetakan 15 orang (39,5%)tingkat kelelahan tertinggi dengan kelelahan kerja terbanyak yaitu kelelahan kerja ringan 27 pekerja (71,1%) Diharapkan bagi pekerja yang terkait yang dapat menyebabkan kelelahan, agar mematuhi segalah aturan yang telah di tetapkan oleh perusahaan.

Kata Kunci      : Kelelahan kerja
Pelatihan Budidaya Curcuma spp dan Pembuatan Olahan Minuman Kesehatan untuk Peningkatan Imunitas
Authors: Rantesigi Nirva , Sufyaningsi Ulfa , Agusrianto Agusrianto
Published: November 2021

WHO menetapkan Corona Virus (nCoV-19) sebagai wabah pendemi global. Ancaman pandemic semakin besar ketika berbagai kasus menunjukkan penularan antar manusia (human to human transmission) pada petugas kesehatan yang merawat pasien, menyebabkan peningkatan jumlah kasus yang luar biasa hingga kasus terkonfirmasi covid 19 sampai Juni 2021 berjumlah 178.837.204. pada kelompok rentan dengan imunitas yang menurun rentan untuk tertular dan menderita infeksi Covid 19, peningkatan imunitas masyarakat penting untuk mencegah dan melawan infeksi. Tujuan kegiatan ini adalah untuk Memberikan pengetahuan dan keterampilan kepada masyarakat dalam  membudidayakan dan mengolah curucuma sebagai salah satu minuman untuk meningkatkan imunitas tubuh. Metode kegiatan ini adalah pelatihan, pembuatan olahan minuman kesehatan dan pembuatan kebun Tanaman Obat Keluarga (TOGA). Hasil kegiatan yaitu meningkatnya pengetahuan tentang manfaat curcuma untuk imunitas, keluarga membuat kebun TOGA dan mampu membuat plahan dari bahan curcuma. Kesimpulan yaitu meningkatnya pengetahuan dan keterampilan masyarakat dalam budidaya dan pengolahan curcuma menjadi minuman kesehatan. Saran bagi petugas kesehatan untuk rutin memberikan pendampingan kepada masyarakat dalam upaya peningkatan imunitas fisik seperti penggunaan bahan alam dimasa pandemi covid.
Edukasi Persiapan Persalinan yang Aman di Era Covid-19
Authors: Hardayanti Dyah Ayu , Ramadhan Kadar , Suharto Dewi Nurviana , Agusrianto Agusrianto
Published: November 2021

Proses persiapan persalinan merupakan suatu proses dimana seorang Ibu yang menanti kelahiran si buah hati yang selama ini dinantikan. Persiapan persalinan dapat dilakukan dengan cara mempersiapkan diri dengan berbagai informasi tentang hal-hal yang menyangkut persalinan, terutama dimasa pandemi saat ini. Ibu hamil pasti akan merasa cemas dan khawatir dalam menghadapi proses kelahiran, sehingga perlu mempersiapkan ibu hamil melalui proses persalinan dengan aman di masa pandemi COVID-19. Tujuan kegiatan pengabdian kepada masyarakat ini yaitu agar ibu lebih siap dan siaga saat proses kelahiran walaupun pada masa pandemi. Metode pengabdian masyarakat yaitu dengan memberikan penyuluhan kepada seorang ibu hamil di desa Matako. Hasil pengabdian menunjukkan bahwa terdapat peningkatan pengetahuan tentang proses persiapan persalinan yang aman di era COVID-19. Kami menyarankan kepada ibu hamil untuk melakukan vaksinasi, mengurangi aktivitas di luar, dan terus menerapkan protokol kesehatan jika mengharuskan beraktivitas di luar rumah.
Edukasi Pencegahan Anemia pada Ibu Hamil
Authors: Abas Istiqamariah , Ramadhan Kadar , Manggasa Dafrosia Darmi , Rantesigi Nirva
Published: November 2021

Anemia yang terjadi pada ibu hamil dapat meningkatkan risiko komplikasi serius, sehingga harus dicegah sedini mungkin. Kegiatan ini bertujuan untuk meningkatkan pengetahuan ibu hamil tentang anemia. Metode kegiatan ini berupa penyuluhan yang diberikan kepada seorang ibu hamil di Kelurahan Lawangan. Kegiatan ini dilaksanakan pada tanggal 14 September 2021. Kegiatan diawali dengan pemberian pretest, kemudian dilanjutkan dengan penyuluhan menggunakan media leaflet. Kegiatan diakhiri dengan pemberian posttest. Hasil kegiatan menunjukan terdapat peningkatan pengetahuan ibu tentang anemia dari 50% sebelum penyuluhan menjadi 90% setelah penyuluhan. Kami menyarankan agar ibu hamil dapat melakukan pencegehan anemia seperti mengonsumsi makanan sumber besi serta perlu melakukan tes kadar hemoglobin darah.
Diabetes Mellitus as a Risk Factor for Trigger Finger –a Longitudinal Cohort Study Over More Than 20 Years
Authors: Löfgren Jin Persson , Zimmerman Malin , Dahlin Lars B. , Nilsson Peter M. , Rydberg Mattias
Published: November 2021

Background and Aim

Trigger finger (TF) or stenosing tenosynovitis has been associated with diabetes mellitus (DM), although today’s knowledge is mostly based on cross-sectional and case-control studies. Thus, the aim of the present population-based cohort study over more than 20 years was to investigate DM as a risk factor for TF.

Methods

Data from Malmö Diet and Cancer Study (MDCS), including 30,446 individuals, were analysed with regards to baseline DM and known or potential confounders. Information regarding TF diagnosis until study end date of Dec 31st, 2018, was retrieved from the Swedish National Patient Register (NPR) using ICD-codes. Survival probability was investigated in Kaplan-Meier plots. Cox proportional hazard regression model was used to evaluate DM as risk factor for TF, adjusted for several confounders and presented as Hazard Ratio (HR) with 95% confidence intervals (CI).

Results

At baseline, 4.6% (1,393/30,357) participants had DM. In total, 3.2% (974/30,357) participants were diagnosed with TF during the study period. Kaplan-Meier plot showed that the probability for incident TF was significantly higher in participants with baseline DM compared with individuals without baseline DM. Adjusted HR for DM as risk factor for TF was 2.0 (95% CI: 1.5-2.6, p<0.001).

Conclusion

This longitudinal study showed that DM is an important risk factor for developing TF. When adjusting for sex, age, BMI, manual work, statin use, smoking and alcohol consumption, DM remained the main risk factor for TF.
Identification of Immune-Related Genes for Establishment of Prognostic Index in Hepatocellular Carcinoma
Authors: Li Yinfang , Zou Ling , Liu Xuejun , Luo Judong , Liu Hui
Published: November 2021

Background: Immune checkpoint inhibitor (ICI) therapy has been proved to be a promising therapy to many types of solid tumors. However, effective biomarker for estimating the response to ICI therapy and prognosis of hepatocellular carcinoma (HCC) patients remains underexplored. The aim of this study is to build a novel immune-related prognostic index based on transcriptomic profiles.

Methods: Weighted gene co-expression network analysis (WGCNA) was conducted to identify immune-related hub genes that are differentially expressed in HCC cohorts. Next, univariate Cox regression analysis and least absolute shrinkage and selection operator (LASSO) analysis were used to detect hub genes associated to overall survival (OS). To validate the immune-related prognostic index, univariate and multivariate Cox regression analysis were performed. CIBERSORT and ESTIMATE were used to explore the tumor microenvironment and immune infiltration level.

Results: The differential expression analysis detected a total of 148 immune-related genes, among which 25 genes were identified to be markedly related to overall survival in HCC patients. LASSO analysis yielded 10 genes used to construct the immune-related gene prognostic index (IRGPI), by which a risk score is computed to estimate low vs. high risk indicating the response to ICI therapy and prognosis. Further analysis confirmed that this immune-related prognostic index is an effective indicator to immune infiltration level, response to ICI treatment and OS. The IRGPI low-risk patients had better overall survival (OS) than IRGPI high-risk patients on two independent cohorts. Moreover, we found that IRGPI high-risk group was correlated with high TP53 mutation rate, immune-suppressing tumor microenvironment, and these patients acquired less benefit from ICI therapy. In contrast, IRGPI-low risk group was associated with low TP53 and PIK3CA mutation rate, high infiltration of naive B cells and T cells, and these patients gained relatively more benefit from ICI therapy.
Preparation of Monodisperse Poly(Methyl Methacrylate)/Polystyrene Composite Particles by Seeded Emulsion Polymerization Using a Sequential Flow Process
Authors: Watanabe Takaichi , Karita Kengo , Manabe Midori , Ono Tsutomu
Published: November 2021

We develop a sequential flow process for the production of monodisperse poly (methyl methacrylate) (PMMA)/polystyrene (PS) composite particles through a soap-free emulsion polymerization of methyl methacrylate (MMA) using the first water-in-oil (W/O) slug flow and a subsequent seeded emulsion polymerization of styrene (St) using the second W/O slug flow. In this process, monodisperse PMMA seed particles are first formed in the dispersed aqueous phase of the first W/O slug flow. Subsequently, removal of the oil phase from the slug flow is achieved through a porous hydrophobic tubing, resulting in a single flow of the aqueous phase containing the seed particles. The aqueous phase is then mixed with an oil phase containing St monomer to form the second W/O slug flow. Finally, monodisperse PMMA/PS composite particles are obtained by a seeded emulsion polymerization of St using the second W/O slug flow. We compared the reaction performance between the slug flow and the batch processes in terms of particle diameter, monomer conversion, particle size distribution, and the number of particles in the system. We found that internal circulation flow within the slugs can enhance mass transfer efficiency between them during polymerization, which results in monodisperse PMMA/PS composite particles with a large particle diameter and a high monomer conversion in a short reaction time, compared to those prepared using the batch process. We believe that this sequential microflow process can be a versatile strategy to continuously produce monodisperse composite particles or core-shell particles in a short reaction time.
Enhanced Migratory Capacity of T Lymphocytes in Severe Chagasic Patients Is Correlated With VLA-4 and TNF-α Expression
Authors: Berbert Luiz Ricardo , González Florencia Belén , Villar Silvina Raquel , Vigliano Carlos , Lioi Susana , Beloscar Juan , Bottasso Oscar Adelmo , Silva-Barbosa Suse Dayse , Savino Wilson , Pérez Ana Rosa
Published: November 2021

Trypanosoma cruzi infection in humans leads to progression to chronic chagasic myocarditis (CCM) in 30% of infected individuals, paralleling T cell inflammatory infiltrates in the heart tissue. T-cell trafficking into the hearts of CCM patients may be modulated by in situ expression of chemotactic or haptotactic molecules, as the chemokine CXCL12, the cytokine tumor necrosis factor-alpha (TNF-α), and extracellular matrix proteins (ECM), such as fibronectin. Herein we evaluated the expression of fibronectin, CXCL12, and TNF-α in the myocardial tissue of T. cruzi seropositive (asymptomatic or with CCM), as well as seronegative individuals as healthy controls. Hearts from CCM patients exhibited enhanced expression of these three molecules. CXCL12 and TNF-α serum levels were also increased in CCM individuals. We then evaluated T lymphocytes from chronic chagasic patients by cytofluorometry, in terms of membrane expression levels of molecules involved in cell activation and cell migration, respectively, HLA-DR and the VLA-4 (very late antigen-4, being one integrin-type fibronectin receptor). Indeed, the expression of HLA-DR and VLA-4 was enhanced on T lymphocytes from chagasic patients, especially in the CCM group. To further approach the dynamics of T cell migratory events, we performed fibronectin-, TNF-α-, and CXCL12-driven migration. Peripheral blood mononuclear cells (PBMCs) and T cells from CCM patients presented an ex vivo enhanced migratory capacity driven by fibronectin alone when this ECM protein was placed in the membrane of transwell migration chambers. When TNF-α was previously placed upon fibronectin, we observed a further and significant increase in the migratory response of both PBMCs and T lymphocytes. Overall, these data suggest the existence in patients with chronic Chagas disease of a cardiac inflammatory infiltrate vector that promotes the recruitment and accumulation of activated T cells, driven in part by enhanced tissue expression of fibronectin and TNF-α, as well as the respective corresponding VLA-4 and TNF receptors.
Enumeration of Viable Non-Culturable Vibrio cholerae Using Droplet Digital PCR Combined With Propidium Monoazide Treatment
Authors: Zhao Shuo , Zhang Jingyun , Li Zhe , Han Yu , Kan Biao
Published: November 2021

Many bacterial species, including Vibrio cholerae (the pathogen that causes cholera), enter a physiologically viable but non-culturable (VBNC) state at low temperature or in conditions of low nutrition; this is a survival strategy to resist environmental stress. Identification, detection, and differentiation of VBNC cells and nonviable cells are essential for both microbiological study and disease surveillance/control. Enumeration of VBNC cells requires an accurate method. Traditional counting methods do not allow quantification of VBNC cells because they are not culturable. Morphology-based counting cannot distinguish between live and dead cells. A bacterial cell possesses one copy of the chromosome. Hence, counting single-copy genes on the chromosome is a suitable approach to count bacterial cells. In this study, we developed quantitative PCR-based methods, including real-time quantitative PCR (qPCR) and droplet digital PCR (ddPCR), to enumerate VBNC V. cholerae cells by counting the numbers of single-copy genes in samples during VBNC-state development. Propidium monoazide (PMA) treatment was incorporated to distinguish dead cells from viable cells. Both PCR methods could be used to quantify the number of DNA copies/mL and determine the proportion of dead cells (when PMA was used). The methods produced comparable counts using three single-copy genes (VC1376, thyA, and recA). However, ddPCR showed greater accuracy and sensitivity than qPCR. ddPCR also allows direct counting without the need to establish a standard curve. Our study develops a PMA-ddPCR method as a new tool to quantify VBNC cells of V. cholerae. The method can be extended to other bacterial species.
Recent Advances in Presentation, Diagnosis and Treatment for Mixed Vaginitis
Authors: Qi Wenhui , Li Huanrong , Wang Chen , Li Huiyang , Zhang Bingbing , Dong Mengting , Fan Aiping , Han Cha , Xue Fengxia
Published: November 2021

Mixed vaginitis is the simultaneous presence of at least two types of vaginitis, contributing to an abnormal vaginal milieu and leading to vaginal symptoms and signs. However, associations between symptoms and the type of mixed vaginitis have not been clearly elucidated, and research on mixed vaginitis is still in the preliminary stage. Therefore, the pathogenic mechanism of mixed vaginitis remains understudied. Mixed vaginitis generally involves the formation of mixed biofilms. The study of polymicrobial interactions and mixed biofilms will provide a new idea for the understanding of mixed vaginitis. Moreover, this review summarizes some effective management and laboratory diagnosis of mixed vaginitis to avoid inappropriate therapy, recurrence, and reinfection. It is of high clinical importance to obtain relevant clinical data to improve clinical knowledge about mixed vaginitis.
Coding Variation and Adherence to Methodological Standards in Cardiac Research Using the National Inpatient Sample
Authors: Ostrominski John W. , Amione-Guerra Javier , Hernandez Brian , Michalek Joel E. , Prasad Anand
Published: November 2021

Background: Code selection is crucial to the accuracy and reproducibility of studies using administrative data, however a comprehensive assessment of coding trends for major cardiac diagnoses and procedures is lacking. We aimed to evaluate trends in administrative code utilization for major cardiac diagnoses and procedures, and adherence to required methodological practices in cardiac research using the National Inpatient Sample (NIS).

Methods: In this observational study of 445 articles, ICD-9-CM codes corresponding to acute myocardial infarction (AMI), heart failure, atrial fibrillation, percutaneous coronary intervention, and coronary artery bypass grafting were collected and analyzed. The NIS was used to compare the number of hospitalizations between the most frequently encountered AMI case definitions. Key elements were abstracted from each article to evaluate adherence to required methodological practices.

Results: Variation in code utilization was observed for each diagnosis and procedure assessed, and the number of unique case definitions published per year increased throughout the study period (P < 0.001), driven largely by the significant increase in articles per year (P < 0.001). Off-target codes were observed in 39 (8.8%) studies. Upon reintroduction into the NIS for 2008–2012, the most commonly encountered case definitions for AMI were found to yield significantly different estimates of AMI hospitalizations and hospitalization trends over time. Three hundred and ninety-nine articles (84%) did not adhere to one or more required research practices. Overall adherence was superior for publications in higher-impact journals (P = 0.002).

Conclusions: Substantial variation in code selection exists for major cardiac diagnoses and procedures, and non-adherence to methodological standards is widespread. These data have important implications for the accuracy and generalizability of analyses using the NIS.
A Novel Resveratrol Analog Upregulates SIRT1 Expression and Ameliorates Neointima Formation
Authors: Yuan Baohui , Liu He , Dong Xiaoliang , Pan Xiaohua , Sun Xun , Sun Jia , Pan Li-Long
Published: November 2021

Neointima formation is a serious complication caused by mechanical trauma to the vessel. (R)-4,6-dimethoxy-3-(4-methoxy phenyl)-2,3-dihydro-1H-indanone [(R)-TML 104] is a synthesized analog of the natural product resveratrol sesquiterpenes (±)-isopaucifloral F. The present study aimed to investigate the effects and underlying mechanisms of (R)-TML104 on neointima formation. Our results showed that (R)-TML104 prevented neointima formation based on a carotid artery injury model in mice. Furthermore, (R)-TML104 inhibited platelet-derived growth factor-BB (PDGF-BB)-induced vascular smooth muscle cells (VSMC) phenotypic transformation, evidenced by increased α-smooth muscle actin, reduced VSMC proliferation, and migration. Simultaneously, (R)-TML104 upregulated sirtuin-1 (SIRT1) expression in VSMC. We further uncovered that SIRT1 expression is critical for the inhibitory effects of (R)-TML104 on PDGF-BB-induced VSMC phenotypic transformation in vitro and injury-induced neointima formation in vivo. Finally, (R)-TML104-upregulated SIRT1 inhibited PDGF-BB-induced VSMC phenotypic transformation by downregulating nicotinamide adenine dinucleotide phosphate oxidase 4 expression via decreasing nuclear factor-κB acetylation. Taken together, these results revealed that (R)-TML104 upregulates SIRT1 expression and ameliorates neointima formation. Therefore, the application of (R)-TML104 may constitute an effective strategy to ameliorate neointima formation.
Omentin-1 Modulates Macrophage Function via Integrin Receptors αvβ3 and αvβ5 and Reverses Plaque Vulnerability in Animal Models of Atherosclerosis
Authors: Lin Xuze , Sun Yan , Yang Shiwei , Yu Mengyue , Pan Liu , Yang Jie , Yang Jiaqi , Shao Qiaoyu , Liu Jinxing , Liu Yan , Zhou Yujie , Wang Zhijian
Published: November 2021

Backgrounds: Omentin-1 is a novel cytokine that is primarily released by the epicardial adipose tissue. Molecular structure analysis revealed that it contained a fibrinogen-like domain. Clinical studies have demonstrated that the expression of omentin-1 is tightly associated with the development of cardiovascular diseases, but the receptor by which omentin-1 modulates macrophage function has not been identified yet.

Objective: This study sought to investigate the effect of omentin-1 on already-established atherosclerosis (AS) lesions in both ApoE−/− and Ldlr−/− mice and further, study its underlying mechanisms.

Methods and Results: We investigated the effect of omentin-1 on the plaque phenotype by implanting a minipump in ApoE−/− and Ldlr−/− mice. In vivo studies showed that the infusion of omentin-1 increased the collagen content and mitigated the formation of the necrotic core in both animal models. Immunohistochemistry and immunofluorescence analysis revealed that omentin-1 suppressed inflammatory cytokines expression, macrophage infiltration, and apoptosis within the plaque. An immunoprecipitation experiment and confocal microscopy analysis confirmed the binding of omentin-1 to the integrin receptors αvβ3 and αvβ5. The cell studies demonstrated that omentin-1 suppressed the apoptosis and inflammatory cytokines expression induced by the oxidized low-density lipoprotein in the macrophage. In addition, omentin-1 promoted the phosphorylation of the integrin-relevant signaling pathway as well as the Akt and AMPK in the macrophage. The addition of the inhibitor of the integrin receptor or interfering with the expression of the integrin subunit αv (ITGAV) both significantly abrogated the bioeffects induced by omentin-1. A flow cytometry analysis indicated that the antibodies against αvβ3 and αvβ5 had a competitive effect on the omentin-1 binding to the cell membrane.

Conclusions: The administration of adipokine omentin-1 can inhibit the necrotic cores formation and pro-inflammatory cytokines expression within the AS lesion. The mechanisms may include the suppression of apoptosis and pro-inflammatory cytokines expression in the macrophage by binding to the integrin receptors αvβ3 and αvβ5.
Case Report: Miles Surgery Ameliorates High Blood Pressure in a Rectal Carcinoma Patient With Essential Hypertension
Authors: Zhang Meng-Wan , Fan Bo-Shi , Yu Jian-Guang
Published: November 2021

Hypertension is one of the major causes of public health problems. Multiple factors affecting gastrointestinal tract function are involved in hypertension. Emerging studies have manifested that gut intervention may play significant roles in regulating blood pressure but the underlying mechanisms are complex and not fully clear. Here, we report a case of 66 years old male who had a long history of hypertension and received Miles surgery for rectal carcinoma. The blood pressure of this patient was returned to normal levels after the operation. The possible reasons could be the modulation of sympathetic tone and the gut microbiota-brain axis. This report provides evidence about the relevance between hypertension and gut intervention particularly in the colorectal sites and gives hints for investigating the possible mechanisms of hypertension and the novel strategy for blood pressure control.
Knowledgeability, Attitude and Practice Behaviors of Dental Providers Toward Provisions of Integrated Care Delivery for Patients With Prediabetes/Diabetes: Wisconsin Statewide Survey
Authors: Shimpi Neel , Panny Aloksagar , Glurich Ingrid , Chyou Po-Huang , Acharya Amit
Published: November 2021

Introduction: To conduct a statewide survey among Wisconsin-based dental providers evaluating current knowledgeability, attitudes and practice behaviors surrounding management of patients with diabetes/prediabetes in the dental setting. The study explored perceptions on feasibility, value, barriers, and current status of integrated care model (ICM) adoption by dental practices

Materials and Methods: A 32-question paper-based survey was mailed to all licensed dentists and dental hygienists practicing in Wisconsin. The study was conducted over a 4 week period in 2019. The survey instrument was adapted from a previous validated survey and was expanded to include questions on ICM adoption. Content and validity analyses and beta testing were conducted prior to dissemination of the survey. Descriptive statistics and chi-square tests were applied for data analyses. Thematic analyses was performed on open-ended questions.

Results: Survey response rate was 12% (N = 854/7,356) representing 41% dentists and 59% dental hygienists. While 68% reported educating patients on oral health-diabetes association, only 18% reported medical consultations to inform dental treatment, and “frequent” (22%) or “occasional” (40%), medical triage. Knowledge-based questions were correctly answered by >70% of participants. While 50% valued chair-side glycemic screening and 85% supported non-invasive chair-side screening to identify at-risk patients,>88% relied on patient-reported diabetic status. Barriers to ICM adoption included time investment (70%), patient activation/cooperation (62%), cost (50%), insurance coverage (50%), infrequent interdisciplinary communication (46%), lack of equipment (33%) and provider (31%).

Conclusion: Low rates of ICM adoption, chair-side testing, medical consultation and triage, and need for educational curricula reform were identified.
Optogenetic Activation of Arcuate Kisspeptin Neurons Generates a Luteinizing Hormone Surge-Like Secretion in an Estradiol-Dependent Manner
Authors: Lin Xian-Hua , Lass Geffen , Kong Ling-Si , Wang Hui , Li Xiao-Feng , Huang He-Feng , O’Byrne Kevin T.
Published: November 2021

Traditionally, the anteroventral periventricular (AVPV) nucleus has been the brain area associated with luteinizing hormone (LH) surge secretion in rodents. However, the role of the other population of hypothalamic kisspeptin neurons, in the arcuate nucleus (ARC), has been less well characterized with respect to surge generation. Previous experiments have demonstrated ARC kisspeptin knockdown reduced the amplitude of LH surges, indicating that they have a role in surge amplification. The present study used an optogenetic approach to selectively stimulate ARC kisspeptin neurons and examine the effect on LH surges in mice with different hormonal administrations. LH level was monitored from 13:00 to 21:00 h, at 30-minute intervals. Intact Kiss-Cre female mice showed increased LH secretion during the stimulation period in addition to displaying a spontaneous LH surge around the time of lights off. In ovariectomized Kiss-Cre mice, optogenetic stimulation was followed by a surge-like secretion of LH immediately after the stimulation period. Ovariectomized Kiss-Cre mice with a low dose of 17β-estradiol (OVX+E) replacement displayed a surge-like increase in LH release during period of optic stimulation. No LH response to the optic stimulation was observed in OVX+E mice on the day of estradiol benzoate (EB) treatment (day 1). However, after administration of progesterone (day 2), all OVX+E+EB+P mice exhibited an LH surge during optic stimulation. A spontaneous LH surge also occurred in these mice at the expected time. Taken together, these results help to affirm the fact that ARC kisspeptin may have a novel amplificatory role in LH surge production, which is dependent on the gonadal steroid milieu.