bluuebunny/crossref_metadata_2025 huggingface dataset:
Supplementation of Ex-Situ Biofloc to Improve Growth Performance and Enhance Nutritional Values of the Pacific White Shrimp Rearing at Low Salinity Conditions
Authors: Uawisetwathana Umaporn , Situmorang Magdalena Lenny , Arayamethakorn Sopacha , Haniswita  , Suantika Gede , Panya Atikorn , Karoonuthaisiri Nitsara , Rungrassamee Wanilada
Published: May 2021

Shrimp is an important food source consumed worldwide. An intensive aquaculture system with overuse of feed in combination with detrimental effects from climate change are serious problems leading to mass mortality of cultured shrimp. Biofloc technology is an approach to managing water quality and controlling the disease to counter the negative side of intensive culture system; however, most of the biofloc applications are naturally formed, which could be inconsistent. In this study, we employed an established optimal ratio of microbial consortium called “ex-situ biofloc (BF)” to be used as a feed supplement in shrimp cultured in a zero-water discharged system at low salinity conditions. Three feeding groups (100%commercial pellet (C), 95%C+BF, 90%C+BF) of shrimp were cultured for six weeks. The effect of an ex-situ biofloc supplement with commercial pellet reduction showed that levels of ammonium, nitrite, nitrate and phosphate were significantly decreased in water culture. Shrimp fed with ex-situ biofloc supplement with commercial pellet reduction exhibited significantly increased shrimp weight and survival, and significantly expressed growth-related genes involving lipolysis and energy metabolism higher than those fed with 100% commercial pellet. Nutritional analysis indicated a significant increase of docosahexaenoic acid (DHA) and eicosenoic acid (C20:1) concentrations in the ex-situ biofloc supplemented shrimp. This finding revealed the potential of ex-situ biofloc to manage water quality, improve shrimp growth performance and enhance shrimp nutritional value under intensive culture at low salinity conditions. The beneficial effects of the ex-situ biofloc in shrimp culture system make it a promising alternative strategy to mitigate climate change effects leading to the sustainable production of high-quality shrimp in the future.
Effect of By-Products from Selected Fruits and Vegetables on Gluten-Free Dough Rheology and Bread Properties
Authors: Djeghim Fairouz , Bourekoua Hayat , Różyło Renata , Bieńczak Agata , Tanaś Wojciech , Zidoune Mohammed Nesreddine
Published: May 2021

The aim of the study was to investigate the effect of using various by-products (orange and apple pomace, tomato peel, pepper peel, prickly pear peel, and prickly pear seed peel) on the dough rheology and properties of gluten-free bread. The by-products were incorporated into a gluten-free bread formulation based on corn and chickpea flours (2/1 w/w). Different levels of each by-product (0, 2.5, 5, and 7.5% in the basic replacement) were tested. Wheat bread and gluten-free bread without the addition of by-products were used as controls. The results indicated that the by-products increased the maximum dough height, the total CO2 production, and CO2 retention coefficient compared to unenriched gluten-free dough. The highest K-value consistency coefficient was observed for the dough enriched with the prickly pear peel. The addition of by-products significantly improved (p < 0.0001) the specific volume of gluten-free bread, with values increasing from 1.48 to 2.50 cm3/g. The hierarchical cluster analysis and the constellation plot showed four groups: the wheat bread group, the second group containing the gluten-free control bread, the group with bread enriched by pomace, and the group with bread enriched with peels, exhibit the same effect on gluten-free bread and the peels exhibit the same effect on gluten-free bread.
Towards a Glass New World: The Role of Ion-Exchange in Modern Technology
Authors: Berneschi Simone , Righini Giancarlo C. , Pelli Stefano
Published: May 2021

Glasses, in their different forms and compositions, have special properties that are not found in other materials. The combination of transparency and hardness at room temperature, combined with a suitable mechanical strength and excellent chemical durability, makes this material indispensable for many applications in different technological fields (as, for instance, the optical fibres which constitute the physical carrier for high-speed communication networks as well as the transducer for a wide range of high-performance sensors). For its part, ion-exchange from molten salts is a well-established, low-cost technology capable of modifying the chemical-physical properties of glass. The synergy between ion-exchange and glass has always been a happy marriage, from its ancient historical background for the realisation of wonderful artefacts, to the discovery of novel and fascinating solutions for modern technology (e.g., integrated optics). Getting inspiration from some hot topics related to the application context of this technique, the goal of this critical review is to show how ion-exchange in glass, far from being an obsolete process, can still have an important impact in everyday life, both at a merely commercial level as well as at that of frontier research.
Energy Management and Control System Design of an Integrated Flywheel Energy Storage System for Residential Users
Authors: Floris Andrea , Porru Mario , Damiano Alfonso , Serpi Alessandro
Published: May 2021

This paper presents the energy management and control system design of an integrated flywheel energy storage system (FESS) for residential users. The proposed FESS is able to draw/deliver 8 kWh at 8 kW, and relies on a large-airgap surface-mounted permanent magnet synchronous machine, the inner rotor of which integrates a carbon-fiber flywheel, leading to a compact and efficient FESS. The proposed energy management system is based on four different operating modes, which are defined and can be selected in accordance with FESS speed and/or user’s preference, while FESS control system is devoted to power/current tracking at both machine- and grid-side converters. The effectiveness of the proposed solutions, as well as the overall energy performance of the proposed FESS, are verified by real-time simulations, which regard different operating conditions and/or realistic scenarios.
An Adaptive Epidemiology-Based Approach to Swarm Foraging with Dynamic Deadlines
Authors: ElGibreen Hebah
Published: May 2021

Swarm robotics is an emerging field that can offer efficient solutions to real-world problems with minimal cost. Despite recent developments in the field, however, it is still not sufficiently mature, and challenges clearly remain. The dynamic deadline problem is neglected in the literature, and thus, time-sensitive foraging tasks are still an open research problem. This paper proposes a novel approach—ED_Foraging—that allows simple robots with limited sensing and communication abilities to perform complex foraging tasks that are dynamic and time constrained. A new mathematical model is developed in this paper to utilize epidemiological modeling and predict the dynamics of resource deadlines. Moreover, an improved dynamic task allocation (DTA) method is proposed to assign robots to the most critical region, where a deadline is represented by a state and time. The main goal is to reduce the number of expired resources and collect them as quickly as possible by giving priority to those that are more likely to expire if not collected. The deadlines are unknown and change dynamically. Thus, the robots continuously collect local information throughout their journeys and allocate themselves dynamically to the predicted hotspots. In the experiments, the proposed approach is adapted to four DTA methods and tested with different setups using simulated foot-bot robots. The flexibility, scalability, and robustness of this approach are measured in terms of the foraging and expiration rates. The empirical results support the hypothesis that epidemiological modeling can be utilized to handle foraging tasks that are constrained by dynamic deadlines. It is also confirmed that the proposed DTA method improves the results, which were found to be flexible, scalable, and robust to changes in the number of robots and the map size.
Synchronous Machine Winding Modeling Method Based on Broadband Characteristics
Authors: Chen Yu , Ji Xiaoqing , Zhao Zhongyong
Published: May 2021

The accurate establishment of the equivalent circuit model of the synchronous machine windings’ broadband characteristics is the basis for the study of high-frequency machine problems, such as winding fault diagnosis and electromagnetic interference prediction. Therefore, this paper proposes a modeling method for synchronous machine winding based on broadband characteristics. Firstly, the single-phase high-frequency lumped parameter circuit model of synchronous machine winding is introduced, then the broadband characteristics of the port are analyzed by using the state space model, and then the equivalent circuit parameters are identified by using an optimization algorithm combined with the measured broadband impedance characteristics of port. Finally, experimental verification and comparison experiments are carried out on a 5-kW synchronous machine. The experimental results show that the proposed modeling method identifies the impedance curve of the circuit parameters with a high degree of agreement with the measured impedance curve, which indicates that the modeling method is feasible. In addition, the comparative experimental results show that, compared with the engineering exploratory calculation method, the proposed parameter identification method has stronger adaptability to the measured data and a certain robustness. Compared with the black box model, the parameters of the proposed model have a certain physical meaning, and the agreement with the actual impedance characteristic curve is higher than that of the black box model.
Numerical Algorithms for Elastoplacity: Finite Elements Code Development and Implementation of the Mohr–Coulomb Law
Authors: Amouzou Gildas Yaovi , Soulaïmani Azzeddine
Published: May 2021

Two numerical algorithms for solving elastoplastic problems with the finite element method are presented. The first deals with the implementation of the return mapping algorithm and is based on a fixed-point algorithm. This method rewrites the system of elastoplasticity non-linear equations in a form adapted to the fixed-point method. The second algorithm relates to the computation of the elastoplastic consistent tangent matrix using a simple finite difference scheme. A first validation is performed on a nonlinear bar problem. The results obtained show that both numerical algorithms are very efficient and yield the exact solution. The proposed algorithms are applied to a two-dimensional rockfill dam loaded in plane strain. The elastoplastic tangent matrix is calculated by using the finite difference scheme for Mohr–Coulomb’s constitutive law. The results obtained with the developed algorithms are very close to those obtained via the commercial software PLAXIS. It should be noted that the algorithm’s code, developed under the Matlab environment, offers the possibility of modeling the construction phases (i.e., building layer by layer) by activating the different layers according to the imposed loading. This algorithmic and implementation framework allows to easily integrate other laws of nonlinear behaviors, including the Hardening Soil Model.
Improving the Dyeability and Anti-Wrinkle Properties of Cotton Fabric via Oxidized Raffinose
Authors: Lou Jiangfei , Zhang Jinfang , Wang Dan , Fan Xuerong
Published: May 2021

In the anti-wrinkle finishing of cotton fabrics, the decreased dyeability of the finished fabrics has always been a difficult problem. A new anti-wrinkle finishing mode was developed to solve this problem by changing the finishing sequence of fabric dyeing and anti-wrinkle. In this research, the partial oxidization of raffinose with sodium periodate generated multiple aldehydes, which acted as multifunctional cross-linkers and endowed cotton fabrics with anti-wrinkle and hydrophilic properties. The structural characteristics of oxyRa were analyzed by FTIR and 13C-NMR. Through response surface methodology (RSM), the finishing model of oxyRa was established from the influencing factors of catalyst concentration, pH, curing temperature and time, and the optimized finishing process: the catalyst concentration was 20.12 g/L, pH was 4.32, curing temperature was 150 °C and curing time was 120 s. Under this condition, the predicted wrinkle recovery angle (WRA) of the finished fabric was up to 249.76°, Tensile strength (TS) was 75.62%, Whiteness index (WI) was 70.69. Importantly, comparing the anti-wrinkle and dyeing performance of the fabric with anti-wrinkle and then dyeing and anti-wrinkle after dyeing, the oxyRa-treated fabrics showed better dyeing properties compared with previously reported dimethyldihydroxyethylene urea (DMDHEU), glutaraldehyde (GA), and 1,2,3,4-butanetetracarboxylic acid (BTCA). Analysis of the combined mechanism of different finishing agents and cellulose, demonstrated the reason why oxyRa can be used to change the order of dyeing and anti-wrinkle finishing.
A Novel Shape Finding Method for the Main Cable of Suspension Bridge Using Nonlinear Finite Element Approach
Authors: Zhu Weiliang , Ge Yaojun , Fang Genshen , Cao Jinxin
Published: May 2021

The determination of the final cable shape under the self-weight of the suspension bridge enables its safe construction and operation. Most existing studies solve the cable shape segment-by-segment in the Lagrangian coordinate system. This paper develops a novel shape finding method for the main cable of suspension bridge using nonlinear finite element approach with Eulerian description. The governing differential equations for a three-dimensional spatial main cable is developed before a one-dimensional linear shape function is introduced to solve the cable shape utilizing the Newton iteration method. The proposed method can be readily reduced to solve the two-dimensional parallel cable shape. Two iteration layers are required for the proposed method. The shape finding process has no need for the information of the cable material or cross section using the present technique. The commonly used segmental catenary method is compared with the present method using three cases study, i.e., a 1666-m-main-span earth-anchored suspension bridge with 2D parallel and 3D spatial main cables as well as a 300-m-main-span self-anchored suspension bridge with 3D spatial main cables. Numerical studies and iteration results show that the proposed shape finding technique is sufficiently accurate and operationally convenient to achieve the target configuration of the main cable.
A Unified Framework for Neuroscience Morphological Data Visualization
Authors: Pastor Luis , Bayona Sofia , Brito Juan , Cuevas María , Fernaud Isabel , Galindo Sergio , García-Cantero Juan , Quevedo Francisco , Mata Susana , Robles Oscar , Rodríguez Angel , Toharia Pablo , Zdravkovic Ana
Published: May 2021

The complexity of the human brain makes its understanding one of the biggest challenges that science is currently confronting. Due to its complexity, the brain has been studied at many different levels and from many disciplines and points of view, using a diversity of techniques for getting meaningful data at each specific level and perspective, producing sometimes data that are difficult to integrate. In order to advance understanding of the brain, scientists need new tools that can speed up this analysis process and that can facilitate integrating research results from different disciplines and techniques. Visualization has proved to be useful in the analysis of complex data, and this paper focuses on the design of visualization solutions adapted to the specific problems posed by brain research. In this paper, we propose a unified framework that allows the integration of specific tools to work together in a coordinated manner in a multiview environment, displaying information at different levels of abstraction and combining schematic and realistic representations. The two use cases presented here illustrate the capability of this approach for providing a visual environment that supports the exploration of the brain at all its organizational levels.
How Different Fermentation Type Affects Volatile Composition of Plum Jerkums
Authors: Januszek Magdalena , Satora Paweł
Published: May 2021

Quality of plum jerkum is significantly associated to the profile of volatile compounds. Therefore, we decided to assess the impact of various fermentation types on selected properties of plum jerkums, especially compounds which contribute to the aroma of the finished product. We used the following yeast strains: S. cerevisiae S1, H. uvarum H2, and Ethanol RED (S. cerevisiae). Moreover, we considered spontaneous fermentation. S. cerevisiae and H. uvarum strains were isolated during the fermentation of Čačanska Lepotica or Węgierka Dąbrowicka (plum cultivars), respectively. As for fermentation type, spontaneous fermentation of H. uvarum H2 provided the best results. It could be associated to the fact that plum juices fermented with H. uvarum H2 presented the highest concentration of terpenoids, esters, or some higher alcohols. In the current paper, application of indigenous strains of yeasts resulted in the required oenological characteristics, e.g., highest fermentation efficiency and concentration of ethanol was determined in juices fermented with Ethanol RED (S. cerevisiae) and also with S. cerevisiae S1. Our results suggested that indigenous strains of yeasts present in plums demonstrate great potential for the production of plum jerkums of high quality.
Retention of the Antibiotic Cefuroxime onto Agricultural and Forest Soils
Authors: Cela-Dablanca Raquel , Nebot Carolina , López Lucia Rodríguez , Ferández-Calviño David , Arias-Estévez Manuel , Núñez-Delgado Avelino , Álvarez-Rodríguez Esperanza , Fernández-Sanjurjo María J.
Published: May 2021

Antibiotics in wastewater, sewage sludge, manures, and slurries constitute a risk for the environment when spread on soils. This work studies the adsorption and desorption of the antibiotic cefuroxime (CFX) in 23 agricultural and forest soils, using batch-type experiments. Our results show that the adsorption values were between 40.75 and 99.57% in the agricultural soils, while the range was lower (from 74.57 to 93.46%) in forest soils. Among the Freundlich, Langmuir, and Linear models, the Freundlich equation shows the best fit for the adsorption results. In addition, agricultural soils with higher pH are the ones that present the highest adsorption. Further confirmation of the influence of pH on adsorption is given by the fact that Freundlich’s KF parameter and the Linear model Kd parameter shows a positive correlation with pH and with the exchangeable Ca and Mg values, which are known to affect the charges of the soil colloids and the formation of cationic bridges between adsorbents and adsorbate. In addition, Freundlich’s n parameter shows a positive and significant correlation with the organic matter content, related to the high adsorption taking place on forest soils despite their pH < 5. Regarding desorption, in most cases, it is lower than 1%, which indicates that CFX is adsorbed in a rather irreversible way onto these soils. Overall, these results can be considered relevant regarding their potential impact on environmental quality and public health.
Association of Mean Daily Polyphenols Intake with Mediterranean Diet Adherence and Anthropometric Indices in Healthy Greek Adults: A Retrospective Study
Authors: Kapolou Aikaterini , Karantonis Haralabos C. , Rigopoulos Nikolaos , Koutelidakis Antonios E.
Published: May 2021

Research data indicate the possible effect of both polyphenols consumption and Mediterranean diet adherence on metabolic diseases’ prevalence. The present retrospective study investigated the possible association of polyphenols mean daily intake with Mediterranean diet adherence and anthropometric indices in a sample of the Greek population. A total of 250 healthy volunteers, aged between 18 and 65 years, were randomly recruited from central and northern Greece. Total daily polyphenols intake was estimated using a semi-quantitative food frequency questionnaire (FFQ) based on the NHANES study, while Med Diet Score was used for the degree of Mediterranean diet adoption. Daily polyphenols intake was identified by the Phenol Explorer database, and anthropometric measurements (BMI, waist-to-hip circumference, and body composition) were performed. The mean daily polyphenols intake was determined to be 1905 mg, while most of the participants had moderate or high mean consumption last year (67.5% of the sample were consuming more than 1000 mg/d). Moderate adherence to the Mediterranean diet (higher Med Diet Score) was associated with increased mean daily polyphenols intake (p = 0.016). Increased polyphenols intake and higher Med Diet Score were associated with decreased waist-to-hip circumference (p = 0.027, 0.004, respectively). Specific functional foods rich in polyphenols, such as sour cherry, tomatoes, black tea, and cocoa were associated with improved body composition indices. Larger epidemiological studies need to be performed for safer conclusions about whole population polyphenols intake and its association with metabolic disease biomarkers.
Improving Air Pollutant Metal Oxide Sensor Quantification Practices through: An Exploration of Sensor Signal Normalization, Multi-Sensor and Universal Calibration Model Generation, and Physical Factors Such as Co-Location Duration and Sensor Age
Authors: Okorn Kristen , Hannigan Michael
Published: May 2021

As low-cost sensors have become ubiquitous in air quality measurements, there is a need for more efficient calibration and quantification practices. Here, we deploy stationary low-cost monitors in Colorado and Southern California near oil and gas facilities, focusing our analysis on methane and ozone concentration measurement using metal oxide sensors. In comparing different sensor signal normalization techniques, we propose a z-scoring standardization approach to normalize all sensor signals, making our calibration results more easily transferable among sensor packages. We also attempt several different physical co-location schemes, and explore several calibration models in which only one sensor system needs to be co-located with a reference instrument, and can be used to calibrate the rest of the fleet of sensor systems. This approach greatly reduces the time and effort involved in field normalization without compromising goodness of fit of the calibration model to a significant extent. We also explore other factors affecting the performance of the sensor system quantification method, including the use of different reference instruments, duration of co-location, time averaging, transferability between different physical environments, and the age of metal oxide sensors. Our focus on methane and stationary monitors, in addition to the z-scoring standardization approach, has broad applications in low-cost sensor calibration and utility.
Degenerate Canonical Forms of Ordinary Second-Order Linear Homogeneous Differential Equations
Authors: Christodoulou Dimitris M. , Kehoe Eric , Katatbeh Qutaibeh D.
Published: May 2021

For each fundamental and widely used ordinary second-order linear homogeneous differential equation of mathematical physics, we derive a family of associated differential equations that share the same “degenerate” canonical form. These equations can be solved easily if the original equation is known to possess analytic solutions, otherwise their properties and the properties of their solutions are de facto known as they are comparable to those already deduced for the fundamental equation. We analyze several particular cases of new families related to some of the famous differential equations applied to physical problems, and the degenerate eigenstates of the radial Schrödinger equation for the hydrogen atom in N dimensions.
Mechanical Sensing Element PDLIM5 Promotes Osteogenesis of Human Fibroblasts by Affecting the Activity of Microfilaments
Authors: Huang Xiaolan , Qu Rongmei , Peng Yan , Yang Yuchao , Fan Tingyu , Sun Bing , Khan Asmat Ullah , Wu Shutong , Wei Kuanhai , Xu Chujiang , Dai Jingxing , Ouyang Jun , Zhong Shizhen
Published: May 2021

Human skin fibroblasts (HSFs) approximate the multidirectional differentiation potential of mesenchymal stem cells, so they are often used in differentiation, cell cultures, and injury repair. They are an important seed source in the field of bone tissue engineering. However, there are a few studies describing the mechanism of osteogenic differentiation of HSFs. Here, osteogenic induction medium was used to induce fibroblasts to differentiate into osteoblasts, and the role of the mechanical sensitive element PDLIM5 in microfilament-mediated osteogenic differentiation of human fibroblasts was evaluated. The depolymerization of microfilaments inhibited the expression of osteogenesis-related proteins and alkaline phosphatase activity of HSFs, while the polymerization of microfilaments enhanced the osteogenic differentiation of HSFs. The evaluation of potential protein molecules affecting changes in microfilaments showed that during the osteogenic differentiation of HSFs, the expression of PDLIM5 increased with increasing induction time, and decreased under the state of microfilament depolymerization. Lentivirus-mediated PDLIM5 knockdown by shRNA weakened the osteogenic differentiation ability of HSFs and inhibited the expression and morphological changes of microfilament protein. The inhibitory effect of knocking down PDLIM5 on HSF osteogenic differentiation was reversed by a microfilament stabilizer. Taken together, these data suggest that PDLIM5 can mediate the osteogenic differentiation of fibroblasts by affecting the formation and polymerization of microfilaments.
Deciphering the Molecular Mechanism of Water Interaction with Gelatin Methacryloyl Hydrogels: Role of Ionic Strength, pH, Drug Loading and Hydrogel Network Characteristics
Authors: Vigata Margaux , Meinert Christoph , Bock Nathalie , Dargaville Bronwin L. , Hutmacher Dietmar W.
Published: May 2021

Water plays a primary role in the functionality of biomedical polymers such as hydrogels. The state of water, defined as bound, intermediate, or free, and its molecular organization within hydrogels is an important factor governing biocompatibility and hemocompatibility. Here, we present a systematic study of water states in gelatin methacryloyl (GelMA) hydrogels designed for drug delivery and tissue engineering applications. We demonstrate that increasing ionic strength of the swelling media correlated with the proportion of non-freezable bound water. We attribute this to the capability of ions to create ion–dipole bonds with both the polymer and water, thereby reinforcing the first layer of polymer hydration. Both pH and ionic strength impacted the mesh size, having potential implications for drug delivery applications. The mechanical properties of GelMA hydrogels were largely unaffected by variations in ionic strength or pH. Loading of cefazolin, a small polar antibiotic molecule, led to a dose-dependent increase of non-freezable bound water, attributed to the drug’s capacity to form hydrogen bonds with water, which helped recruit water molecules in the hydrogels’ first hydration layer. This work enables a deeper understanding of water states and molecular arrangement at the hydrogel–polymer interface and how environmental cues influence them.
Hypoxia Engineered Bone Marrow Mesenchymal Stem Cells Targeting System with Tumor Microenvironment Regulation for Enhanced Chemotherapy of Breast Cancer
Authors: Zu Jingzhi , Tan Liwei , Yang Li , Wang Qi , Qin Jing , Peng Jing , Jiang Hezhong , Tan Rui , Gu Jian
Published: May 2021

Improving the tumor targeting of docetaxel (DTX) would not only be favored for the chemotherapeutic efficacy, but also reduce its side effects. However, the regulation of the tumor microenvironment could further inhibit the growth of tumors. In this study, we introduced a system consisting of hypoxia-engineered bone marrow mesenchymal stem cells (H-bMSCs) and DTX micelles (DTX-M) for breast cancer treatment. First, the stem cell chemotherapy complex system (DTX@H-bMSCs) with tumor-targeting ability was constructed according to the uptake of DTX-M by hypoxia-induced bMSCs (H-bMSCs). DTX micellization improved the uptake efficiency of DTX by H-bMSCs, which equipped DTX@H-bMSCs with satisfactory drug loading and stability. Furthermore, the migration of DTX@H-bMSCs revealed that it could effectively target the tumor site and facilitate the drug transport between cells. Moreover, in vitro and in vivo pharmacodynamics of DTX@H-bMSCs exhibited a superior antitumor effect, which could promote the apoptosis of 4T1 cells and upregulate the expression of inflammatory factors at the tumor site. In brief, DTX@H-bMSCs enhanced the chemotherapeutic effect in breast cancer treatment.
A Symmetric Three Degree of Freedom Tensegrity Mechanism with Dual Operation Modes for Robot Actuation
Authors: Wang Tianyuan , Post Mark A.
Published: May 2021

Tensegrity robots that use bio-inspired structures have many superior properties over conventional robots with regard to strength, weight, compliance and robustness, which are indispensable to planetary exploration and harsh environment applications. Existing research has presented various tensegrity robots with abundant capabilities in broad scenarios but mostly not focused on articulation and manipulability. In this paper, we propose a novel tensegrity mechanism for robot actuation which greatly improves the agility and efficiency compared with existing ones. The design integrates two separate tensegrity substructures inspired by shoulder and hip joints of the human body and features a similar form to a hexapod platform. It mitigates detrimental antagonistic forces in the structural network for optimising actuation controllability and efficiency. We validated the design both on a prototype and in a Chrono Engine simulation that represents the first physically accurate simulation of a wheeled tensegrity robot. It can reach up to approximately 58.9∘, 59.4∘ and 47.1∘ in pitch, yaw and roll motion, respectively. The mechanism demonstrates good agility and controllability as an actuated robot linkage while preserving desirable properties of tensegrity structures. The design would potentially inspire more possibilities of agile tensegrity implementations that enable future robots with enhanced compliance, robustness and efficiency without a tradeoff.
Is the Age of Developmental Milestones a Predictor for Future Development in Down Syndrome?
Authors: Locatelli Chiara , Onnivello Sara , Antonaros Francesca , Feliciello Agnese , Filoni Sonia , Rossi Sara , Pulina Francesca , Marcolin Chiara , Vianello Renzo , Toffalini Enrico , Ramacieri Giuseppe , Martelli Anna , Procaccini Giulia , Sperti Giacomo , Caracausi Maria , Pelleri Maria Chiara , Vitale Lorenza , Pirazzoli Gian Luca , Strippoli Pierluigi , Cocchi Guido , Piovesan Allison , Lanfranchi Silvia
Published: May 2021

Down Syndrome (DS) is the most common genetic alteration responsible for intellectual disability, which refers to deficits in both intellectual and adaptive functioning. According to this, individuals with Down Syndrome (DS) reach developmental milestones (e.g., sitting, walking, and babbling) in the same order as their typically developing peers, but later in life. Since developmental milestones are the first blocks on which development builds, the aims of the current study are to: (i) expand the knowledge of developmental milestone acquisition; and (ii) explore the relationship between developmental milestone acquisition and later development. For this purpose 105 children/adolescents with DS were involved in this study, divided in two groups, Preschoolers (n = 39) and School-age participants (n = 66). Information on the age of acquisition of Sitting, Walking, Babbling, and Sphincter Control was collected, together with cognitive, motor, and adaptive functioning. Sitting predicted later motor development, but, with age, it became less important in predicting motor development in everyday life. Babbling predicted later language development in older children. Finally, Sphincter Control emerged as the strongest predictor of motor, cognitive, language, and adaptive skills, with its role being more evident with increasing age. Our data suggest that the age of reaching the milestones considered in the study has an influence on successive development, a role that can be due to common neural substrates, the environment, and the developmental cascade effect.
Changes in the Brain Activity and Visual Performance of Patients with Strabismus and Amblyopia after a Compete Cycle of Light Therapy
Authors: Ibrahimi Danjela , Mendiola-Santibañez Jorge , Cruz-Martínez Enoé , Gómez-Espinosa Alfonso , Torres-Pacheco Irineo
Published: May 2021

This research assesses the brain activity and visual performance at baseline and after light therapy (LTH), of seventeen patients with strabismus and amblyopia (SA), and eleven healthy controls (HCs) from Querétaro, México. Quantitative electroencephalogram analysis (qEEG) was used to record the brain activity, and clinical metrics such as the visual acuity, angle of deviation, phoria state, stereopsis, and visual fields determined the visual performance. Results showed a constant higher alpha-wave frequency for HCs. Low voltages remained negative for HCs and positive for SA patients across stimulation. After LTH, high voltage increased in SA patients, and decreased in HCs. A second spectral peak, (theta-wave), was exclusively recorded in SA patients, at baseline and after LTH. Positive Spearman correlations for alpha-wave frequency, low and high voltages were only seen in SA patients. Synchronized brain activity was recorded in all SA patients stimulated with filters transmitting light in the blue but not in the red spectrum. Enhancement in the visual performance of SA patients was found, whereas deterioration of the phoria state and a decrease in the amount of stereopsis was seen in HCs. To conclude, only a suffering brain and a visual pathway which needs to be enabled can benefit from LTH.
The Participation of Microglia in Neurogenesis: A Review
Authors: Pérez-Rodríguez Diego R. , Blanco-Luquin Idoia , Mendioroz Maite
Published: May 2021

Adult neurogenesis was one of the most important discoveries of the last century, helping us to better understand brain function. Researchers recently discovered that microglia play an important role in this process. However, various questions remain concerning where, at what stage, and what types of microglia participate. In this review, we demonstrate that certain pools of microglia are determinant cells in different phases of the generation of new neurons. This sheds light on how cells cooperate in order to fine tune brain organization. It also provides us with a better understanding of distinct neuronal pathologies.
Web-Based Assessment of the Phenomenology of Autobiographical Memories in Young and Older Adults
Authors: Vannucci Manila , Chiorri Carlo , Favilli Laura
Published: May 2021

Autobiographical memories (ABMs) produce rich phenomenological experiences. Although few standardized and comprehensive measures of the phenomenology of ABMs have been developed, a web-based assessment of the full range of phenomenological properties is still missing. In the present study, we aimed to fill this gap and tested the psychometric properties of a web-based version of the Assessment of the Phenomenology of Autobiographical Memory (APAM) in a group of young and older adults. Specifically, taking advantage of the flexibility of web-based assessment methodology, we tested the rating consistency of APAM items, asking participants to rate the phenomenology of their ABMs with respect to seven cues, administered in one per day in seven different days. In each session, we also collected ratings of mood and arousal. Using linear mixed modeling (LMM), we could examine whether the phenomenology ratings differed with respect to age group while controlling for sex, age of the memory, arousal, mood, and specificity of the memory. Results revealed an adequate level of consistency of ratings in both young and older adults. Moreover, LMMs revealed a more intense experience of recollection and reliving (i.e., sensory and emotional) and a higher confidence in memory accuracy in older compared to younger adults. The theoretical and practical usefulness of a web-based assessment of the phenomenology of ABMs are discussed.
Mesoporous Organo-Silica Supported Chromium Oxide Catalyst for Oxidative Dehydrogenation of Ethane to Ethylene with CO2
Authors: Al-Awadi Abdulrhman S. , El-Toni Ahmed Mohamed , Labis Joselito P. , Khan Aslam , Ghaithan Hamid , Al-Zahrani Attiyah A. , Abasaeed Ahmed E. , Al-Zahrani Saeed M.
Published: May 2021

Chromium oxide supported on mesoporous organo-silica (MOS) was synthesized with different Cr loading by an incipient method. The catalytic performance of a Cr(x)/MOS catalyst for CO2-based ethane dehydrogenation was investigated. The synthesized catalysts were characterized by XRD, BET, TEM, SEM, XPS, FTIR, and UV–Vis DR measurements. The textural properties of the prepared samples showed that the mesoporous nature of MOS sample was not disturbed by chromium impregnation. Among the prepared samples, Cr(8)/MOS catalyst exhibited good distribution of chromium species along with superior concentration of Cr6+ and the highest recorded Cr6+/Cr3+ ratio. The results revealed that the superior catalytic performance was reached at Cr(8)/MOS, with 50.4% and 90.1% of ethane conversion and ethylene selectivity, respectively. The catalytic activity decreased slowly over reaction time; it declined approximately 22% after 10 h of stream operation. The roles of CO2-based ethane dehydrogenation were also studied, where carbon dioxide can be a source of lattice oxygen and as a hydrogen consumer in reverse water–gas shift (RWGS) reaction. The effect of various catalytic factors, such as catalytic temperature, reaction time, space gas velocity, and CO2 partial pressure on the conversion of ethane, yield, and selectivity to ethylene, were investigated as well.
IL-10 Mediated Immunomodulation Limits Subepithelial Fibrosis and Repairs Airway Epithelium in Rejecting Airway Allografts
Authors: Khan Mohammad Afzal , Ashoor Ghazi Abdulmalik , Shamma Talal , Alanazi Fatimah , Altuhami Abdullah , Kazmi Shadab , Ahmed Hala Abdalrahman , Mohammed Assiri Abdullah , Clemens Broering Dieter
Published: May 2021

Interleukin-10 plays a vital role in maintaining peripheral immunotolerance and favors a regulatory immune milieu through the suppression of T effector cells. Inflammation-induced microvascular loss has been associated with airway epithelial injury, which is a key pathological source of graft malfunctioning and subepithelial fibrosis in rejecting allografts. The regulatory immune phase maneuvers alloimmune inflammation through various regulatory modulators, and thereby promotes graft microvascular repair and suppresses the progression of fibrosis after transplantation. The present study was designed to investigate the therapeutic impact of IL-10 on immunotolerance, in particular, the reparative microenvironment, which negates airway epithelial injury, and fibrosis in a mouse model of airway graft rejection. Here, we depleted and reconstituted IL-10, and serially monitored the phase of immunotolerance, graft microvasculature, inflammatory cytokines, airway epithelium, and subepithelial collagen in rejecting airway transplants. We demonstrated that the IL-10 depletion suppresses FOXP3+ Tregs, tumor necrosis factor-inducible gene 6 protein (TSG-6), graft microvasculature, and establishes a pro-inflammatory phase, which augments airway epithelial injury and subepithelial collagen deposition while the IL-10 reconstitution facilitates FOXP3+ Tregs, TSG-6 deposition, graft microvasculature, and thereby favors airway epithelial repair and subepithelial collagen suppression. These findings establish a potential reparative modulation of IL-10-associated immunotolerance on microvascular, epithelial, and fibrotic remodeling, which could provide a vital therapeutic option to rescue rejecting transplants in clinical settings.
Fibrous Demineralized Bone Matrix (DBM) Improves Bone Marrow Mononuclear Cell (BMC)-Supported Bone Healing in Large Femoral Bone Defects in Rats
Authors: Verboket René D. , Irrle Tanja , Busche Yannic , Schaible Alexander , Schröder Katrin , Brune Jan C. , Marzi Ingo , Nau Christoph , Henrich Dirk
Published: May 2021

Regeneration of large bone defects is a major objective in trauma surgery. Bone marrow mononuclear cell (BMC)-supported bone healing was shown to be efficient after immobilization on a scaffold. We hypothesized that fibrous demineralized bone matrix (DBM) in various forms with BMCs is superior to granular DBM. A total of 65 male SD rats were assigned to five treatment groups: syngenic cancellous bone (SCB), fibrous demineralized bone matrix (f-DBM), fibrous demineralized bone matrix densely packed (f-DBM 120%), DBM granules (GDBM) and DBM granules 5% calcium phosphate (GDBM5%Ca2+). BMCs from donor rats were combined with different scaffolds and placed into 5 mm femoral bone defects. After 8 weeks, bone mineral density (BMD), biomechanical stability and histology were assessed. Similar biomechanical properties of f-DBM and SCB defects were observed. Similar bone and cartilage formation was found in all groups, but a significantly bigger residual defect size was found in GDBM. High bone healing scores were found in f-DBM (25) and SCB (25). The application of DBM in fiber form combined with the application of BMCs shows promising results comparable to the gold standard, syngenic cancellous bone. Denser packing of fibers or higher amount of calcium phosphate has no positive effect.
Knockout of the CMP–Sialic Acid Transporter SLC35A1 in Human Cell Lines Increases Transduction Efficiency of Adeno-Associated Virus 9: Implications for Gene Therapy Potency Assays
Authors: Banning Antje , Zakrzewicz Anna , Chen Xin , Gray Steven J. , Tikkanen Ritva
Published: May 2021

Recombinant adeno-associated viruses (AAV) have emerged as an important tool for gene therapy for human diseases. A prerequisite for clinical approval is an in vitro potency assay that can measure the transduction efficiency of each virus lot produced. The AAV serotypes are typical for gene therapy bind to different cell surface structures. The binding of AAV9 on the surface is mediated by terminal galactose residues present in the asparagine-linked carbohydrates in glycoproteins. However, such terminal galactose residues are rare in cultured cells. They are masked by sialic acid residues, which is an obstacle for the infection of many cell lines with AAV9 and the respective potency assays. The sialic acid residues can be removed by enzymatic digestion or chemical treatment. Still, such treatments are not practical for AAV9 potency assays since they may be difficult to standardize. In this study, we generated human cell lines (HEK293T and HeLa) that become permissive for AAV9 transduction after a knockout of the CMP–sialic acid transporter SLC35A1. Using the human aspartylglucosaminidase (AGA) gene, we show that these cell lines can be used as a model system for establishing potency assays for AAV9-based gene therapy approaches for human diseases.
Prediction of Sodium Substitution Sites in Octacalcium Phosphate: The Relationships of Ionic Pair Ratios in Reacting Solutions
Authors: Sugiura Yuki , Horie Masanori
Published: May 2021

Octacalcium phosphate (OCP) is widely used in biomaterial fabrication by virtue of its unique crystal structure and low environmental loading. Although various ion and molecule substitution methods into the OCP unit lattice have been introduced, it remains unclear which factors and mechanisms dominate the substitution process. Experimental studies have indicated that Na alkali metal ions are substituted at the P3 PO4 conjugated site in acidic to weakly acidic conditions and the P5 PO4 conjugated site in neutral to weak basic conditions. Ionic species calculation methods have indicated that the pair ratios of Na and HPO42− (NaHPO4−) are small in acidic reacting solutions but large under weakly basic conditions. Consequently, the roles played by NaHPO4− and ionic pair formation processes are thought to dominate ion and molecule substitution into the OCP unit lattice. Such ionic pair formation strongly inhibits dicarboxylic acid substitution into the OCP unit lattice due to the replacement of the Ca ion, which conjugates P5 PO4 as an anchor of dicarboxylic acid.
Protective Effect of Resveratrol against Hepatotoxicity of Cadmium in Male Rats: Antioxidant and Histopathological Approaches
Authors: Al-Baqami Najah , Hamza Reham
Published: May 2021

Cadmium (Cd) is widely used in some industries and emitted from fossil fuels. It is a heavy metal with a number of side effects, including hepatotoxicity. Resveratrol (Rs) is considered an important polyphenol, which is a secondary plant metabolite and has the ability to scavenge free radicals. The study was designed to evaluate the effects of resveratrol on Cd, which induced hepatotoxicity, by the assessment of some histopathological and biochemical alterations. Forty male albino rats were divided into four groups: the 1st group was the control group, the 2nd group was treated with Cd (5 mg/kg), the 3rd group was given Rs (20 mg/kg), and the 4th group was treated with Cd in combination with Rs intraperitoneally for 30 successive days. The results indicate that Cd increased liver enzymes alanine aminotransferase and aspartate aminotransferase (AST and ALT), alkaline phosphatase ALP and gamma-glutamyl transferase (γ-GT) while reducing the total protein level; Cd increased the malondialdhyde (MDA) level while decreasing the levels of other antioxidant enzymes super oxide dismutase, catalase and glutathione peroxidase (SOD, CAT and GPx). Serious congestion and hemorrhage related to the hepatic tissues were noticed in the Cd group, and Rs plays a major role in alleviating histopathological injuries and hepatic oxidative damage. It is clear that Rs has the ability to minimize the hepatotoxicity induced by Cd in male rats.
Microstructure and Corrosion Behavior of Ti-Nb Coatings on NiTi Substrate Fabricated by Laser Cladding
Authors: Hu Jie , Ren Yaojia , Huang Qianli , He Hao , Liang Luxin , Liu Jingbo , Li Ruidi , Wu Hong
Published: May 2021

Ti-23Nb (at.%) coatings on an NiTi alloy with metallurgical bonding were prepared by laser cladding (LC) technology using Ti-Nb mixture powders. The effects of laser processing parameters on the microstructure and mechanical properties of the coatings were systematically investigated and the corrosion resistance of the coatings was assessed. The coatings were composed of TiNb, (Ti, Nb)2Ni, and β-Nb phases. The coatings increased the hardness of the NiTi alloy by a combined strengthening effect of the eutectics and fine microstructure. The corrosion resistance of the coated part was improved. The coatings with great corrosion resistance could keep the coated parts inert in an aggressive environment, and effectively restrain the release of toxic Ni ions, which means that the Ti-Nb alloy coatings are likely to be used as a biomaterial for medical applications.