bluuebunny/crossref_metadata_2025 huggingface dataset:
Effects of two-week machine massage on muscle properties in adolescent wrestlers
Authors: Qu Guangcai , Wang Hongbo , Zhou Guohai , Liu Haiping
Published: February 2023

Objective: This study aimed to investigate the effect of a two-week machine massage on the physical properties of the erector spinae and serum biochemical indexes of adolescent athletes after training.

Methods: Sixteen male adolescent wrestlers were recruited (age: 15 ± 1 year; height: 166 ± 7 cm; weight: 56 ± 7 kg) and randomly assigned to machine massage (MA, 8) and control (CO, 8) groups. Participants in the MA group received machine massage for 20 min after each wrestling training from Monday to Saturday (except on Thursday) for two weeks, while the participants in the CO group recovered naturally. Over the course of two weeks, all the participants underwent similar wrestling training program under the guidance of a professional coach. Before and after the intervention, serum urea and creatine kinase (CK) levels were measured in a fasting state. A Myoton Pro digital muscle evaluation system was used to measure the physical properties of the erector spinae, including the oscillation frequency, logarithmic decrement of a muscle’s natural oscillation, and dynamic stiffness.

Results: After two weeks of machine massage treatment, the dynamic stiffness of the erector spinae in the MA group decreased by 12.90% and that in the CO group increased by 2.34%, indicating a significant difference between the two groups (p = 0.04, ƞ2 = 0.286). The decrease in the logarithmic decrement of a muscle’s natural oscillation value in the MA was significantly greater than that in the CO (p = 0.003, ƞ2 = 0.286). Moreover, the serum CK values decreased by 33.84% in the MA group and by 1.49% in the CO group, despite a trend of change between the groups (p = 0.062, ƞ2 = 0.084). No significant difference was found in the improvement in serum urea levels between the two groups after two weeks of treatment.

Conclusion: Results of the present study indicated that a two-week machine massage had a positive effect on the improvement of the physical properties of the erector spinae of wrestlers during training.
Functional characterization of maternally accumulated hydrolases in the mature oocytes of the vector Rhodnius prolixus reveals a new protein phosphatase essential for the activation of the yolk mobilization and embryo development
Authors: de Almeida Elisa , Dittz Uilla , Pereira Jéssica , Walter-Nuno Ana B. , Paiva-Silva Gabriela O. , Lacerda-Abreu Marco A. , Meyer-Fernandes Jose R. , Ramos Isabela
Published: February 2023

Yolk biogenesis and consumption have been well conserved in oviparous animals throughout evolution. Most egg-laying animals store yolk proteins within the oocytes’ yolk granules (Ygs). Following fertilization, the Ygs participate in controlled pathways of yolk breakdown to support the developing embryo’s anabolic metabolism. While the unfolding of the yolk degradation program is a crucial process for successful development in many species, the molecular mechanisms responsible for yolk mobilization are still mysterious and have mostly not been explored. Here, we investigate the functional role of the oocyte maternally accumulated mRNAs of a protein phosphatase (PP501) and two aspartic proteases (cathepsin-D 405, CD405 and cathepsin-D 352, CD352) in the yolk degradation and reproduction of the insect vector of Chagas disease Rhodnius prolixus. We found that PP501 and CD352 are highly expressed in the vitellogenic ovary when compared to the other organs of the adult insect. Parental RNAi silencing of PP501 resulted in a drastic reduction in oviposition and increased embryo lethality whereas the silencing of CD352 resulted only in a slight decrease in oviposition and embryo viability. To further investigate the PP501-caused high reproduction impairment, we investigated the Ygs biogenesis during oocyte maturation and the activation of the yolk degradation program at early development. We found that the Ygs biogenesis was deficient during oogenesis, as seen by flow cytometry, and that, although the PP501-silenced unviable eggs were fertilized, the Ygs acidification and acid phosphatase activity were affected, culminating in a full impairment of the yolk proteins degradation at early embryogenesis. Altogether we found that PP501 is required for the oocyte maturation and the activation of the yolk degradation, being, therefore, essential for this vector reproduction.
Optical imaging spectroscopy coupled with machine learning for detecting heavy metal of plants: A review
Authors: Li Junmeng , Ren Jie , Cui Ruiyan , Yu Keqiang , Zhao Yanru
Published: February 2023

Heavy metal elements, which inhibit plant development by destroying cell structure and wilting leaves, are easily absorbed by plants and eventually threaten human health via the food chain. Recently, with the increasing precision and refinement of optical instruments, optical imaging spectroscopy has gradually been applied to the detection and reaction of heavy metals in plants due to its in-situ, real-time, and simple operation compared with traditional chemical analysis methods. Moreover, the emergence of machine learning helps improve detection accuracy, making optical imaging spectroscopy comparable to conventional chemical analysis methods in some situations. This review (a): summarizes the progress of advanced optical imaging spectroscopy techniques coupled with artificial neural network algorithms for plant heavy metal detection over ten years from 2012-2022; (b) briefly describes and compares the principles and characteristics of spectroscopy and traditional chemical techniques applied to plants heavy metal detection, and the advantages of artificial neural network techniques including machine learning and deep learning techniques in combination with spectroscopy; (c) proposes the solutions such as coupling with other analytical and detection methods, portability, to address the challenges of unsatisfactory sensitivity of optical imaging spectroscopy and expensive instruments.
Photosynthetic photon flux density affects fruit biomass radiation-use efficiency of dwarf tomatoes under LED light at the reproductive growth stage
Authors: Ke Xinglin , Yoshida Hideo , Hikosaka Shoko , Goto Eiji
Published: February 2023

This study aimed to analyze the effects of photosynthetic photon flux density (PPFD) on fruit biomass radiation-use efficiency (FBRUE) of the dwarf tomato cultivar ‘Micro-Tom’ and to determine the suitable PPFD for enhancing the FBRUE under LED light at the reproductive growth stage. We performed four PPFD treatments under white LED light: 200, 300, 500, and 700 μmol m−2 s−1. The results demonstrated that a higher PPFD led to higher fresh and dry weights of the plants and lowered specific leaf areas. FBRUE and radiation-use efficiency (RUE) were the highest under 300 μmol m−2 s−1. FBRUE decreased by 37.7% because RUE decreased by 25% and the fraction of dry mass portioned to fruits decreased by 16.9% when PPFD increased from 300 to 700 μmol m−2 s−1. Higher PPFD (500 and 700 μmol m−2 s−1) led to lower RUE owing to lower light absorptance, photosynthetic quantum yield, and photosynthetic capacity of the leaves. High source strength and low fruit sink strength at the late reproductive growth stage led to a low fraction of dry mass portioned to fruits. In conclusion, 300 µmol m−2 s−1 PPFD is recommended for ‘Micro-Tom’ cultivation to improve the FBRUE at the reproductive growth stage.
Exogenous 6-BA inhibited hypocotyl elongation under darkness in Picea crassifolia Kom revealed by transcriptome profiling
Authors: Liu Hongmei , Zhou Chengcheng , Nisa Zaib Un , El-Kassaby Yousry A. , Li Wei
Published: February 2023

Hypocotyl elongation is an important process in plant growth and development, and is under hormonal regulatory signaling pathways. In our study, exogenous 6-BA significantly inhibited Picea crassifolia hypocotyl elongation more than ethylene in the dark, indicating the existence of different regulatory strategies in conifers, therefore, the P. crassifolia transcriptome was studied to explore the responsive genes and their regulatory pathways for exogenous N6-benzyladenine (6-BA) inhibition of hypocotyl elongation using RNA-Sequencing approach. We present the first transcriptome assembly of P. crassifolia obtained from 24.38 Gb clean data. With lowly-expressed and short contigs excluded, the assembly contains roughly 130,612 unigenes with an N50 length of 1,278 bp. Differential expression analysis found 3,629 differentially expressed genes (DEGs) and found that the differential expression fold of genes was mainly concentrated between 2 and 8 (1 ≤ log2FoldChange ≤ 3). Functional annotation showed that the GO term with the highest number of enriched genes (83 unigenes) was the shoot system development (GO: 0048367) and the KEGG category, plant hormone signal transduction (ko04075), was enriched 30 unigenes. Further analysis revealed that several cytokinin dehydrogenase genes (PcCTD1, PcCTD3 and PcCTD6) catabolized cytokinins, while xyloglucan endotransglucosylase hydrolase gene (PcXTH31), WALLS ARE THIN 1-like gene (PcWAT1-1) and Small auxin-induced gene (PcSAUR15) were strongly repressed thus synergistically completing the inhibition of hypocotyl elongation in P. crassifolia. Besides, PcbHLH149, PcMYB44 and PcERF14 were predicted to be potential core TFs that may form a multi-layered regulatory network with the above proteins for the regulation of hypocotyl growth.
VPS45 is required for both diffuse and tip growth of Arabidopsis thaliana cells
Authors: Mugume Yosia , Roy Rahul , Agbemafle William , Shepard Gabriella N. , Vue Yee , Bassham Diane C.
Published: February 2023

Introduction

VPS45 belongs to the Sec1/Munc18 family of proteins, which interact with and regulate Qa-SNARE function during membrane fusion. We have shown previously that Arabidopsis thaliana VPS45 interacts with the SYP61/SYP41/VTI12 SNARE complex, which locates on the trans-Golgi network (TGN). It is required for SYP41 stability, and it functions in cargo trafficking to the vacuole and in cell expansion. It is also required for correct auxin distribution during gravitropism and lateral root growth.

Results

As vps45 knockout mutation is lethal in Arabidopsis, we identified a mutant, vps45-3, with a point mutation in the VPS45 gene causing a serine 284-to-phenylalanine substitution. The VPS45-3 protein is stable and maintains interaction with SYP61 and SYP41. However, vps45-3 plants display severe growth defects with significantly reduced organ and cell size, similar to vps45 RNAi transgenic lines that have reduced VPS45 protein levels. Root hair and pollen tube elongation, both processes of tip growth, are highly compromised in vps45-3. Mutant root hairs are shorter and thicker than those of wild-type plants, and are wavy. These root hairs have vacuolar defects, containing many small vacuoles, compared with WT root hairs with a single large vacuole occupying much of the cell volume. Pollen tubes were also significantly shorter in vps45-3 compared to WT.

Discussion

We thus show that VPS45 is essential for proper tip growth and propose that the observed vacuolar defects lead to loss of the turgor pressure needed for tip growth.
A model worker: Multifaceted modulation of AUXIN RESPONSE FACTOR3 orchestrates plant reproductive phases
Authors: Fu Yunze , Zhang Hao , Ma Yuru , Li Cundong , Zhang Ke , Liu Xigang
Published: February 2023

The key phytohormone auxin is involved in practically every aspect of plant growth and development. Auxin regulates these processes by controlling gene expression through functionally distinct AUXIN RESPONSE FACTORs (ARFs). As a noncanonical ARF, ARF3/ETTIN (ETT) mediates auxin responses to orchestrate multiple developmental processes during the reproductive phase. The arf3 mutation has pleiotropic effects on reproductive development, causing abnormalities in meristem homeostasis, floral determinacy, phyllotaxy, floral organ patterning, gynoecium morphogenesis, ovule development, and self-incompatibility. The importance of ARF3 is also reflected in its precise regulation at the transcriptional, posttranscriptional, translational, and epigenetic levels. Recent studies have shown that ARF3 controls dynamic shoot apical meristem (SAM) maintenance in a non-cell autonomous manner. Here, we summarize the hierarchical regulatory mechanisms by which ARF3 is regulated and the diverse roles of ARF3 regulating developmental processes during the reproductive phase.
Improving the efficacy of plant-made anti-HIV monoclonal antibodies for clinical use
Authors: Grandits Melanie , Grünwald-Gruber Clemens , Gastine Silke , Standing Joseph F. , Reljic Rajko , Teh Audrey Y-H. , Ma Julian K-C.
Published: February 2023

Introduction

Broadly neutralising antibodies are promising candidates for preventing and treating Human Immunodeficiency Virus/Acquired Immunodeficiency Syndrome (HIV/AIDS), as an alternative to or in combination with antiretroviral therapy (ART). These mAbs bind to sites on the virus essential for virus attachment and entry, thereby inhibiting entry into the host cell. However, the cost and availability of monoclonal antibodies, especially combinations of antibodies, hampers implementation of anti-HIV bNAb therapies in low- to middle- income countries (LMICs) where HIV-1 prevalence is highest.

Methods

We have produced three HIV broadly neutralizing antibodies (bNAbs), 10-1074, VRC01 and 3BNC117 in the Nicotiana benthamiana transient expression system. The impact of specific modifications to enhance potency and efficacy were assessed. To prolong half-life and increase bioavailability, a M252Y/S254T/T256E (YTE) or M428L/N434S (LS) mutation was introduced. To increase antibody dependent cellular cytotoxicity (ADCC), we expressed an afucosylated version of each antibody using a glycoengineered plant line.

Results

The majority of bNAbs and their variants could be expressed at yields of up to 47 mg/kg. Neither the expression system nor the modifications impacted the neutralization potential of the bNAbs. Afucosylated bNAbs exhibit enhanced ability to bind to FcγRIIIa and trigger ADCC, regardless of the presence of Fc amino acid mutations. Lastly, we demonstrated that Fc-modified variants expressed in plants show enhanced binding to FcRn, which results in a favourable in vivo pharmacokinetic profile compared to their unmodified counterparts.

Conclusion

Tobacco plants are suitable expression hosts for anti-HIV bNAbs with increased efficacy and an improved pharmacokinetic profile.
Disgust sensitivity in early pregnancy as a response to high pathogen risk
Authors: Kaňková Šárka , Takács Lea , Hlaváčová Jana , Calda Pavel , Monk Catherine , Havlíček Jan
Published: February 2023

Introduction

Considered a part of the behavioral immune system (BIS), disgust sensitivity is expected to be adjusting as a response to the actual level of the environmental health risks.

Methods

In this preregistered study, we tested the hypothesis that disgust sensitivity would be higher during the COVID-19 pandemic compared to the pre-pandemic period in pregnant women. In this between-subject study with a longitudinal trend design, we administered the Disgust Scale-Revised to 200 pregnant women before the pandemic and to 350 pregnant women during the pandemic.

Results

We found a small but significant effect of the pandemic on disgust sensitivity, such that higher disgust sensitivity was found in women pregnant during the pandemic. This effect was stronger in primiparae, however, the interaction between parity and the pandemic period was not significant. Disgust sensitivity decreased with age. No differences in terms of nausea and vomiting were found between the women pregnant before and during the pandemic.

Discussion

Our findings indicate that although BIS is presumed to function as a complex mechanism to prevent health-threatening behaviors, its activation in pregnant women during the COVID-19 pandemic is rather weak.
Are you more risk-seeking when helping others? Effects of situational urgency and peer presence on prosocial risky behavior
Authors: Liu Changlin , Xiao Xiao , Pi Qiao , Tan Qianbao , Zhan Youlong
Published: February 2023

Introduction

Prosocial risky behavior (PRB) proposes that individuals take risks for others’ benefits or social welfare, and that this may involve trade-offs between risk and social preferences. However, little is known about the underlying cognitive mechanisms of risk-seeking or aversion during PRB.

Methods

This study adopted the dilemma-priming paradigm to examine the interaction between the risk levels of personal cost and situational urgency on PRB (Experiment 1, N  = 88), and it further uncovered the modulation of the risk levels of failure (Experiment 2, N  = 65) and peer presence (Experiment 3, N  = 80) when helping others.

Results

In Experiment 1, the participants involved in risky dilemmas made more altruistic choices for strangers in urgent situations compared to those for strangers in non-urgent situations. However, increasing the risk levels of personal cost decreased the frequencies of help offered to strangers in urgent situations. Experiment 2 further established that, similar to the risk of personal cost, increasing the risk levels of failure when helping others also decreased the frequencies of help offered to strangers in urgent situations. Furthermore, in dilemmas involving a low-risk personal cost, Experiment 3 showed that peer presence encouraged the participants to make more altruistic choices when providing help to strangers in non-urgent situations.

Discussion

Individuals demonstrate obvious risk-seeking behavior when helping others and that both non-urgent situations and peer presence weaken the effect of increased risk aversion on PRB in a limited manner.
Gender-specific association of blood lipids and reproductive trajectory with cognitive impairment: A community based cross-sectional study from India
Authors: Khate Kevingu , Chaudhary Vineet , Longkumer Imnameren , Saraswathy Kallur Nava , Devi Naorem Kiranmala
Published: February 2023

Background

Abnormal blood lipid levels in the general population and adverse reproductive events among women have been associated with cognitive impairment (CI). However, their relationship has not been extensively studied in community settings. Hence, this study aims to explore the association of CI with blood lipid levels in both sexes and reproductive events/trajectory among women.

Methods

A cross-sectional study was conducted among a North Indian rural population. A total of 808 adults were recruited through door-to-door household survey. Data on socio-demographic variables, reproductive profile of women, and cognitive impairment status were collected. Fasting blood sample was collected to estimate serum lipid profile. Multivariate logistic regression was performed to test for association.

Results

The study demonstrated a lack of association between lipid profile and cognitive impairment among males. Surprisingly, low HDL-C among females was found to be protective against moderate/severe cognitive impairment (value of p = 0.049). Further, menopausal women and those having five or higher live births were found to be at higher risk of CI than pre-menopausal women and those with 1–2 live births, respectively.

Conclusion

The present study hints toward a gender-specific association of blood lipid levels with CI. Further, higher live births and menopause appear to be important risk factors for CI among women.
Story telling in bilingual Urdu−Cantonese ethnic minority children: Macrostructure and its relation to microstructural linguistic skills
Authors: Chan Angel , Chen Sarah , Hamdani Saboor , Tse Bernard , Cheng Kelly
Published: February 2023

Introduction

The ability to produce a well-structured, coherent and informative narrative requires the integration of lexical and grammatical skills at different levels of complexity. Investigating how narrative macrostructure competence is predicted by microstructural linguistic skills is conceptually enlightening; yet there have been very few, if any, studies documenting the associations between macrostructure and microstructure in both languages of the same bilinguals. In this paper we attempt to address this research gap and report on the first empirical study of Urdu-Cantonese bilingual children’s narrative abilities, bringing in data from a new language pair that is currently understudied.

Methods

Twenty-four bilinguals (mean age = 9.17 years) acquiring Urdu as first, family and heritage minority language, and Cantonese as second, school and majority language were assessedviaMultilingual Assessment Instrument for Narratives (MAIN). We examined these children’s macrostructural competence and its relations to microstructural skills in both languages (Urdu and Cantonese). Three macrostructure components were scored as response variables: Story Structure (SS), Story Complexity (SC), Internal State Terms (IST). Four microstructural measures were scored as predictor variables: number of different words (NDW), mean length of Communication Units (MLCU), proportion of grammatical Communication Units (Gproportion), proportion of correct connectives linking the major episodic elements (Cproportion).

Results

In regression analyses, NDW emerged consistently as a positive predictor of SS, SC and IST in both languages. MLCU and NDW were positive predictors of SS in the stronger L1, but NDW was the only positive predictor of SS in L2. By contrast, NDW and an index of syntactic competence (MLCU in L1, but Cproportion in L2) were significant or close-to-significant positive predictors of SC in both languages. NDW was the only positive predictor of IST in both languages. These findings suggested that the relationships between narrative macrostructure and specific microstructural abilities could manifest both similarly and differently between L1 and L2.

Discussion

We discuss the findings by considering the unique nature of each macrostructure component and how each component might be related to specific microstructural linguistic skills. We suggest directions for further research and discuss how the current findings bring deeper implications for educators and clinicians in assessment, pedagogy, and intervention.
Comparing time focus with time importance for measuring future time perspectives in the context of pro-environmental values and outcomes
Authors: Olsen Svein Ottar , Tuu Ho Huy , Tudoran Ana Alina
Published: February 2023

Introduction

In the area of environmental psychology, time and the temporal perspective have often been used as an overarching framework to explain or predict environmental outcomes. This study aims to introduce a new Time Importance Scale (TIS) based on the attitude strength theory and to assess its nomological validity in comparison with the more established Temporal Focus Scale (TFS) in the context of consumers’ willingness to pay more for environmentally friendly products. The new TIS scale is short and simple to manage.

Method

The study proposes competing plausible structural models testing alternative relationships between future time perspective (FTP) and environmental values using a nationwide representative survey sample of 633 Norwegians.

Results

The results confirm the convergent and discriminant validity of the new TIS scale. However, the direct association between the TIS scale and willingness to pay for environmentally friendly products is weak or non-significant depending on the alternative models used to validate the nomological validity.

Discussion

The new TIS scale provides evidence of a possible causal chain, FTP → environmental values → willingness to pay, with strong associations between the present TIS and hedonic values and between the future TIS and biospheric values. Environmental values are activated by FTP. In most cases, the new TIS outperforms the established TFS in nomological validity. Future research should validate our findings in experimental trials to demonstrate more substantial causal relationships.
Obsessive-compulsive symptoms in patient with schizophrenia: The influence of disorganized symptoms, duration of schizophrenia, and drug resistance
Authors: Panov Georgi , Panova Presyana
Published: February 2023

Background

Schizophrenia is a chronic mental disorder with a many-faced clinical presentation. Obsessive–compulsive symptoms are often part of it. The characteristics of the clinical picture and the course of schizophrenia are factors related to both the resistance and the manifestation of obsessive–compulsive symptoms. Our study aims to establish the relationship between the peculiarities of the schizophrenia process and the influence of resistance on the expression of obsessive–compulsive symptoms.

Methods

A study was conducted on 105 patients with schizophrenia. Of them, 39 are men and 66 are women. The evaluation of the effectiveness of the treatment showed that 45 were resistant to the applied therapy, while the remaining 60 responded. Clinical assessment of patients was performed using the Positive and Negative Syndrome Scale (PANSS) and Brief Psychiatric Rating Scale (BPRS). Assessment of obsessive–compulsive symptoms (OCS) was conducted with the Dimensional obsessive–compulsive symptoms scale (DOCS).

Results

In 34% of all patients, we found clinically expressed obsessive–compulsive symptoms. In 40% of the patients with resistance, we found clinically expressed obsessive–compulsive symptoms, which are within the range of moderately expressed. In 30% of the patients in clinical remission, we found obsessive–compulsive symptoms, but mildly expressed. We found a statistically significant relationship between the severity of OCS and the disorganized symptoms and the duration of the schizophrenia process. No differences were found in the expression of OCS in patients of both sexes.

Conclusion

We registered both an increased frequency and an increased expression of obsessive–compulsive symptoms in patients with resistant schizophrenia. These symptoms were positively associated with disorganized symptoms and duration of schizophrenia. No relationship was established with the positive, negative symptoms, as well as with the gender distribution.
“I was bullied for being fat in every situation, in every outfit, at every celebration”: A qualitative exploratory study on experiences of weight-based oppression in Qatar
Authors: O'Hara Lily , Alajaimi Bayan , Alshowaikh Bayan
Published: February 2023

Introduction

Weight-based oppression (WBO) has been documented as a widespread phenomenon in Western countries and is associated with a range of psychological, physiological, and behavioral harms. Research on weight-based oppression is largely absent from the Arab region.

Methods

We conducted a qualitative exploratory study using semi-structured in-depth interviews to examine the internalized attitudes, values, and beliefs related to body weight, and experiences of external weight-based oppression of 29 staff, faculty, and students at Qatar University.

Results

Thematic analysis revealed six major themes on the characteristics of internalized WBO, and the nature, timing, source, extent, and impact of external WBO. WBO was regarded as so common in the Arab culture as to be normative, with damaging exposure to WBO beginning in early childhood.

Conclusion

WBO in the Arab region is an important and unrecognized public health issue. Programs to reduce WBO should be developed in all sectors.
Psychological workplace violence and its influence on professional commitment among nursing interns in China: A multicenter cross-sectional study
Authors: Yu Zixu , Kong Dong , Li Yaqin , Zhang Jie , Guo Aiwen , Xie Qi , Gao Feng , Luan Xiaoli , Zhuang Xin , Du Chunling , Liu Jin
Published: February 2023

Background

Psychological workplace violence (WPV) is the primary form of workplace violence suffered by nursing interns. Psychological WPV not only damages the physical and mental health of nursing interns, but also has a negative impact on their work quality and career choice.

Aim

To investigate the characteristics and types of psychological WPV suffered by nursing interns in China, analyze the influencing factors of psychological WPV among nursing interns, and explore the influence of psychological WPV on the professional commitment of nursing interns.

Methods

The subjects were 1,095 nursing interns from 14 medical colleges in Shandong Province. The data were collected electronically using the psychological WPV against nursing interns questionnaire and the professional commitment scale of nursing. The frequency and component ratio were used to describe the incidence and characteristics of psychological WPV. Binary logistic regression was used to analyze the influencing factors of psychological WPV, and linear regression investigated the influence of psychological WPV on the professional commitment of nursing interns.

Results

In the study, 45.0% (n = 493) of nursing interns suffered at least one incidence of psychological WPV during clinical practice, mainly discrimination and verbal abuse. Patients and their relatives were the main perpetrators of psychological WPV. Discrimination and lack of trust were the two main reasons behind psychological WPV. Furthermore, 75.9% of psychological WPV incidents were not effectively reported. Logistic regression showed that clinical internship duration, place of family residence, and hospital level were the influencing factors of psychological WPV among nursing interns. Linear regression results showed that psychological WPV had a negative effect on nursing interns' professional commitment.

Conclusion

Psychological WPV against nursing interns is highly prevalent in China, negatively impacting their professional commitment. It is suggested that colleges should introduce courses for nursing interns to understand and cope with psychological WPV before entering clinical practice, and hospitals should establish a mechanism to prevent, cope with, report, and deal with psychological WPV to effectively reduce the incidence of psychological WPV against nursing interns, improve their ability to cope with psychological WPV, and enhance their professional commitment.
Housing policies by young people, not for young people. Experiences from a co-creation project in Amsterdam
Authors: Hoekstra Joris , Gentili Martina
Published: February 2023

For young adults on the Amsterdam housing market the accessibility of housing has been decreasing for years, due to soaring house prices and rents, the shrinkage and residualization of the social rental sector, and the precarization of the labor market. Consequently, many young people struggle to secure an affordable and adequate dwelling and are stuck in insecure and chaotic housing pathways. Current housing policies in Amsterdam are struggling to effectively respond to these challenges. In an effort to better understand and address the specific housing problems of young people, the Municipality of Amsterdam, housing association Lieven de Key, resident organization !Woon, Delft University of Technology and a group of local young people have started a co-creation process within the framework of the H2020 UPLIFT project. The goal of this co-creation process is to unravel the real-life experiences of young people and to co-create new or improved policy initiatives with them. This paper examines the results of said policy co-creation process in order to evaluate its methodology as well as its impact on the participating actors - young people in particular - and on the policymaking approach. We analyze the benefits and limits of this type of participatory practice in addressing housing issues and try to draw conclusions on its applicability in a larger context.
Ultrasound-assisted extraction of polyphenols from Chinese propolis
Authors: Peng Shiqin , Zhu Meifei , Li Shanshan , Ma Xiaobin , Hu Fuliang
Published: February 2023

Introduction

Propolis is a beneficial bioactive food with rich polyphenols content. Nowadays, an increasing interest is attracted to the extraction of polyphenols from raw propolis. This study utilized the novel ultrasound-assisted approach for polyphenol extraction from Chinese propolis, aiming to improve its extraction yield and reveal the relevant mechanisms via extraction kinetic study as well as the compositional and structural analysis.

Methods

The optimum ultrasound-assisted extraction conditions were optimized according to the total phenolic content and total flavonoids content. Compositional and structural analysis were conducted using high performance liquid chromatography-quadrupole-time of flight tandem mass spectrometry, high-performance liquid chromatography, Fourier-transform infrared spectroscopy (FT-IR) and scanning electron microscope (SEM).

Results and discussion

The optimum ultrasound-assisted extraction conditions were as follows: ratio of liquid to solid, 60:1; ultrasound power, 135 W; ultrasound duration, 20 min. Under the optimum conditions, the antioxidant activities of the extract were increased by 95.55% and 64.46% by 2,2-diphenyl-1-picrylhydrazyl radical scavenging ability assay and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging ability assay, respectively, compared to those obtained by traditional maceration. The second-order kinetics model was employed to study the extraction process; it was found that ultrasound significantly accelerated the extraction of propolis and increased the maximum extraction volume of phenolic compounds. The qualitative and quantitative analysis of polyphenol compositions showed that ultrasound did not change the polyphenol types in the extract but it significantly improved the contents of various flavonoids and phenolic acids such as galangin, chrysin, pinocembrin, pinobanksin and isoferulic acid. Likewise, the FT-IR analysis indicated that the types of functional groups were similar in the two extracts. The SEM analysis revealed that the ultrasound-assisted extraction enhanced the contact areas between propolis and ethanol by breaking down the propolis particles and eroding the propolis surface.
OECD workshop consensus report: Ethical considerations with respect to human derived products, specifically human serum, in OECD test guidelines
Authors: Jacobs Miriam N. , Bult Jan M. , Cavanagh Kevin , Chesne Christophe , Delrue Nathalie , Fu Jianan , Grange Emma , Langezaal Ingrid , Misztela Dominika , Murray Jenny , Paparella Martin , Stoddart Gilly , Tonn Torsten , Treasure Carol , Tsukano Masaaki , Versteegen Rosemary
Published: February 2023

The ethical needs and concerns with use and sourcing of human materials, particularly serum, in OECD in vitro test guidelines were explored in a dedicated international workshop held in 2019. The health-related aspects of the donation procedure, including tissue screening, donor health, laboratory work health protection, permission from the donor for commercial use, payment of the donors and the potential for exploitation of low-income populations and data protection of the donors; supply, availability, and competition with clinical needs; traceability of the serum and auditability/GLP needs for the Test Guideline Programme, were examined. Here we provide the recommendations of the workshop with respect to the use of human serum, and potentially other human reagents, specifically with regard to test method development for OECD Test Guideline utility as part of the Mutual Acceptance of Data requirement across all OECD member countries. These include informed donor consent terminology, a checklist of human serum information requirements to be included with the Good Laboratory Practise report, and suitable sources for human serum to ensure waste supplies are used, that can no longer be used for medical purposes, ensuring no competition of supply for essential medical use.
Defining ovine dermal papilla cell markers and identifying key signaling pathways regulating its intrinsic properties
Authors: Wang Shanhe , Hu Tingyan , He Mingliang , Gu Yifei , Cao Xiukai , Yuan Zehu , Lv Xiaoyang , Getachew Tesfaye , Quan Kai , Sun Wei
Published: February 2023

Dermal papilla cell (DPC), one of the key cell types during hair follicle development and regeneration, specifies hair size, shape and cycling. It is also an important in vitro screening model for hair growth. Although some characteristics of DPCs, such as agglutinative growth and marker genes, have been studied in mice and humans, the intrinsic properties of ovine DPCs and the regulatory mechanism of the intrinsic properties during continued culture in vitro remained unknown. In this study, based on our previous single-cell transcriptome sequencing on sheep lambskin, we verified SOX18 and PDGFRA as the novel marker genes of ovine DPCs through immunofluorescence staining on skin sections and cultured DPCs. Using continued cell culture and alkaline phosphatase staining, we found that different from mice and humans, ovine DPCs exhibit particularly robust and stable aggregation with unbated alkaline phosphatase activity till 30 passages during continued culture in vitro. Also, we found that the expression of some marker genes and the activity of Wnt/β-catenin signaling differ between early passaged DPCs and multiple passaged DPCs. Further, using Wnt/β-catenin agonist and antagonist, we demonstrated that Wnt/β-catenin signaling could regulate cell aggregation and alkaline phosphatase activity of ovine DPCs through regulating FGF and IGF signaling. This study provides the basis for isolating ovine DPCs and defines their intrinsic properties, which contribute to improving wool performance and medicine of hair regeneration.
Wavefront Control Strategies for Large Active Thin Shell Primaries with Unimorph Actuators
Authors: Wang Kainan , Yu Yian , Preumont André
Published: February 2023

This paper presents various aspects of the wavefront control strategies for an ultra-lightweight composite reflector made of polymers for the large primary of a space telescope, and the shape control is made by a set of patterned unimorph strain actuators attached to the reflector. It starts with an analytical investigation of the mechanical behaviors of a strain-actuated curved shell, resulting in the accurate prediction of typical features, such as the damped wave deformation at the transition between electrodes and the limited morphing amplitude of a “print-through” actuation, which indicates that the curvature-induced rigidity deteriorates the performances of the forming accuracy of the active reflector and the morphing stroke of the actuators. The morphing capabilities are evaluated with both petal-like segmented and monolithic configured reflectors by numerical tests on forming target shapes of Zernike modes with various patternings of electrodes, and the structural dynamics are examined. Finally, a compound control strategy is proposed, which uses a deformable relay mirror to compensate for the residual surface error corrected partially by the active unimorph primary mirror, showing a great relaxation of the shape error budget of the thin-shell primary, especially for on-axis observation.
A Numerical Study on the Transient Injection Characteristics of Gas Fuel Injection Devices for Direct-Injection Engines
Authors: Wang Tianbo , Wang Hongchen , Zhang Lanchun , Zheng Yan , Li Li , Chen Jing , Gong Wu
Published: February 2023

Natural gas has emerged as one of the preferred alternative fuels for vehicles owing to its advantages of abundant reserves, cleaner combustion and lower cost. At present, the gas supply methods for natural-gas engines are mainly port fuel injection (PFI) and direct injection (DI). The transient injection characteristics of a gas fuel injection device, as the terminal executive component of the PFI or DI mode, will directly affect the key performance of a gas fuel engine. Therefore, gas fuel injection devices have been selected as the research object of this paper, with a focus on the transient injection process. To explore the impacts of valve vibration amplitude, period, frequency and velocity on transient injection characteristics, one transient computational fluid dynamics (CFD) model for gas fuel injection devices was established. The findings thereof demonstrated that there is a linear relationship between the instantaneous mass flow rate and instantaneous lift during the vibration process. However, this relationship is somewhat impacted when the valve speed is high enough. A shorter valve vibration period tends to preclude a shorter period of flow-hysteresis fluctuation. The near-field pressure fluctuation at the throat of an injection device, caused by valve vibration, initiates flow fluctuation.
Automatic Calibration of Tool Center Point for Six Degree of Freedom Robot
Authors: Lin Chih-Jer , Wang Hsing-Cheng , Wang Cheng-Chi
Published: February 2023

The traditional tool center point (TCP) calibration method requires the operator to use their experience to set the actual position of the tool center point. To address this lengthy workflow and low accuracy, while improving accuracy and efficiency for time-saving and non-contact calibration, this paper proposes an enhanced automatic TCP calibration method based on a laser displacement sensor and implemented on a cooperative robot with six degrees of freedom. During the calibration process, the robot arm will move a certain distance along the X and Y axes and collect the information when the tool passes through the laser during the process to calculate the runout of the tool, and then continue to move a certain distance along the X and Y axes for the second height calibration. After the runout angle is calculated and calibrated by triangulation, the runout calibration is completed and the third X and Y axis displacement is performed to find out the exact position of the tool on the X and Y axes. Finally, the tool is moved to a position higher than the laser, and the laser is triggered by moving downward to obtain information to complete the whole experimental process and receive the calibrated tool center position. The whole calibration method is, firstly, verified in the virtual simulation environment and then implemented on the actual cooperative robot. The results of the proposed TCP calibration method for the case of using a pin tool can achieve a positioning deviation of 0.074 and 0.125 mm for the robot moving speeds of 20 and 40 mm/s, respectively. The orientation deviation in the x-axis are 0.089 and −0.184 degrees for the robot moving speeds of 20 and 40 mm/s, respectively. The positioning repeatability of ±0.083 mm for the moving speed of 20 mm/s is lower than ±0.101 mm for the speed of 40 mm/s. It shows that lower moving speed can obtain higher accuracy and better repeatability. This result meets the requirements of TCP calibration but also achieves the purpose of being simple, economical, and time-saving, and it takes only 60 s to complete the whole calibration process.
Petri Nets Applied in Purge Algorithm Analysis for a Rocket Engine Test with Liquid Propellant
Authors: Bortoloto Evandro Rostirolla , Bizarria Francisco Carlos Parquet , Bizarria José Walter Parquet
Published: February 2023

During the development stage of a space vehicle, instrumented tests are carried out on the ground to prove the operational capacity of each liquid-propellant rocket engine, which is installed in this type of vehicle. The task of elaborating a Test Bench project for a propulsion unit with this application is complex and involves several steps, one of these steps being related to the analysis of this bench capacity to meet the algorithms for the liquid-propellant rocket-engine full run of tests, which is considered fundamental for this project’s operational success. Due to the high costs involved in this project’s elaboration and execution, it is strategic to use computational resources to evaluate, by simulation, the main operational functionalities that are previously established for this bench to perform. In this context, this work presents a model proposal through Petri Nets to evaluate, by computer simulation, an architecture capacity that was designed for the Test Bench to meet an algorithm dedicated to the liquid-propellant pipelines purge during the run of hot tests with the liquid-propellant rocket engine. The method used in this work to carry out the simulation shows the operational response of each module of this architecture, in accordance with the steps contained in the purge algorithm, which allows for analyzing, for each event of the process, the Petri Nets properties, mainly those related to the conservativeness, liveliness, deadlock-type, and confusion-type conflicts. The simulation carried out with the proposed model allows for the portrayal of the physical architecture and the operational states of the purge system according to the steps foreseen in the algorithm, showing that the conservation property is met because the number of marks remains constant, the vivacity property is also met since all positions have been reached, and there is no mortal-type conflict, as the simulation is not stopped; only confusion-type conflict is identified, which was solved with the strategic insertion of resources in the model in order to fix crashes related to the competition for tokens in the transition-enabled entries. The satisfactory results obtained in these simulations suggest that the modules provided for this architecture are sufficient and appropriate for carrying out all the steps contained in the purge algorithm, which will minimize or even eliminate the disorders that may be caused by the presence of foreign elements in the propellant supply lines during the tests with the rocket engine.
The Introduction of Sustainable Aviation Fuels—A Discussion of Challenges, Options and Alternatives
Authors: Grimme Wolfgang
Published: February 2023

The aviation industry is challenged to reduce its climate impact. The introduction of sustainable aviation fuels (SAF) is, among other policy instruments such as the European Emissions Trading Scheme, an option favored by policymakers in Europe to achieve this objective. These fuels feature substantially reduced carbon life-cycle emissions in comparison to fossil fuels. In Europe, a mandatory quota for the use of sustainable fuels will most likely be introduced, starting in the year 2025. The introduction of a blending mandate by governments and the European Commission is associated with a range of challenges. The purpose of this paper is to discuss the economics of climate change mitigation in aviation and the role SAFs can play. The economic issues associated with the introduction of SAFs are analyzed, with a particular focus on the European Commission’s proposal for a blending mandate. Several suggestions for improvement are discussed. Furthermore, alternatives to SAFs are presented and evaluated.
GNSS-Denied Semi-Direct Visual Navigation for Autonomous UAVs Aided by PI-Inspired Inertial Priors
Authors: Gallo Eduardo , Barrientos Antonio
Published: February 2023

This article proposes a method to diminish the horizontal position drift in the absence of GNSS (Global Navigation Satellite System) signals experienced by the VNS (Visual Navigation System) installed onboard a UAV (Unmanned Air Vehicle) by supplementing its pose estimation non-linear optimizations with priors based on the outputs of the INS (Inertial Navigation System). The method is inspired by a PI (Proportional Integral) control loop, in which the attitude and altitude inertial outputs act as targets to ensure that the visual estimations do not deviate past certain thresholds from their inertial counterparts. The resulting IA-VNS (Inertially Assisted Visual Navigation System) achieves major reductions in the horizontal position drift inherent to the GNSS-Denied navigation of autonomous UAVs. Stochastic high-fidelity Monte Carlo simulations of two representative scenarios involving the loss of GNSS signals are employed to evaluate the results and to analyze their sensitivity to the terrain type overflown by the aircraft. The authors release the C++ implementation of both the navigation algorithms and the high-fidelity simulation as open-source software.