bluuebunny/crossref_metadata_2025 huggingface dataset:
Dual career through the eyes of university student-athletes in the Republic of Kosovo
Authors: Gjaka Masar , Feka Kaltrina , Tessitore Antonio , Thomas Abbey , Capranica Laura
Published: May 2024

Introduction

The successful combination of a sports career and education, known as the dual career, requires cooperation and a multi-dimensional approach. Although extensive research has been conducted on dual career programs and services in developed countries, there is limited information available on the dual-career phenomenon in developing countries. This study aimed to explore the dual career experiences of university student-athletes in the Republic of Kosovo.

Materials and methods

A 30-item online survey was distributed to student-athletes, addressing various aspects of the dual career. A total of 121 student-athletes (males: 63.6%; females: 36.4%) voluntarily participated in the survey.

Results

These student-athletes represented 13 different sports, with 102 (84.3%) participating in team and 19 (15.7%) in individual sports. Between individual and team sports no significant differences were found regarding university and sports engagement, and the time required to travel from the university to the training venue, whereas a significant difference (p = 0.019) emerged for the time needed to travel from home to the training venue. Significant differences (p < 0.05) were found between university majors in terms of sports engagement. The present findings highlight a lack of familiarity with dual career programs among student-athletes (89.3%) and the need for dual-career policies at the university (16.5%), sport (9.9%), and national (13.2%) levels. Student-athletes faced various challenges, mainly related to limited leisure time (62.8%), academic overload (60.3%), frequent absence from classes (59.6%), and financial uncertainty (35.5%). Recommended improvements included increased financial support (66.1%), the availability of sports facilities at or near the university (48.8%), greater educational flexibility (26.4%), tutoring services at the university (25.6%), and sports clubs (19%) levels, as well as improved communication regarding existing initiatives and legal aspects (53.7% and 47.1%, respectively). The study also identified parents (98.3%), coaches (86%), and siblings (60.3%) as important sources of support for student-athletes at personal, sports, and university levels.

Conclusions

In conclusion, to enhance the potential of future student-athletes in the Republic of Kosovo, relevant stakeholders in sports and higher education should collaborate closely and implement programs and services based on international best practices for dual-career support.
Exoskeleton-assisted training to enhance lower limb motor recovery in subacute stroke: does timing matter? A pilot randomized trial
Authors: Schröder Jonas , Yperzeele Laetitia , Embrechts Elissa , Loureiro-Chaves Renata , Hallemans Ann , Lafosse Christophe , Truijen Steven , Kwakkel Gert , Saeys Wim
Published: May 2024

Background

Lower limb motor recovery, including abnormal muscle synergies, occurs mainly within the first 5–8 weeks after a stroke. This suggests the importance of delivering impairment-focused therapies, such as therapeutic robots that promote symmetric gait, during this time-sensitive period, following the principle of “the earlier, the better.”

Objective

First, to compare early robotic training (ERT) with usual care (UC) against UC alone on restoring intralimb muscle synergies and interlimb symmetry during functional tasks; Second, to investigate whether ERT is superior to delayed robotic training (DRT) starting after the proposed time-sensitive period.

Methods

This observer-blinded, randomized pilot trial with crossover design involved 19 nonambulatory adults included within 14 days poststroke. Those allocated to ERT (N = 10) received immediately 4 weeks of training (16 sessions, 4× /week) with the Ekso GT® above UC and were compared with the DRT group (N = 9) who received UC alone at this point. Thereafter a 3-week UC period followed to investigate sustainability of ERT and the interventional roles were exchanged; at about week 8 poststroke DRT subjects started the same experimental robotic protocol and ERT subjects continued UC as controls. Outcomes included changes in Fugl-Meyer lower extremity scores (FM-LE) reflecting muscle synergies, weight-bearing asymmetry (WBA), and dynamic control asymmetry (DCA) during quiet standing. Functional ambulation category (FAC) was used to classify walking independence (cut-off ≥4).

Results

A trend toward earlier reacquisition of walking independence favoring ERT with UC over UC was not accompanied by differences in FM-LE, WBA, or DCA (first objective). Thereafter, DRT with UC did not yield any significant changes relative to UC, such that no between-group differences were found favoring restorative effects of ERT over DRT (second objective).

Conclusion

This pilot trial shows the feasibility of investigating a wearable exoskeleton as an adjunct therapy in subacute stroke. Nevertheless, our preliminary findings suggest that motor recovery of lower limb muscle synergies was not enhanced by 4 weeks of robotic training to reduce compensations with the less-affected side, irrespective of the timing of application.

Trial registration

ClinicalTrials.gov, identifier: NCT03727919.
Impact of Climate Change on the Development of Viticulture in Central Poland: Autoregression Modeling SAT Indicator
Authors: Maciejewska Daria , Olewnicki Dawid , Stangierska-Mazurkiewicz Dagmara , Tyminski Marcin , Latocha Piotr
Published: May 2024

Ongoing climate change is having a profound impact on agriculture, which is attracting attention from the scientific community. One of its effects is an increase in average temperature, which is a key factor in grape cultivation. This may increase the popularity of viticulture in central Europe. The aim of this study was to assess the potential for the development of viticulture in central Poland based on SAT changes from 1975 to 2021, in addition to changes in evapotranspiration, occurrence of late spring and early autumn frosts and frosty days in selected years from this period as an important factors relating to climate change. The research utilized data obtained from the Institute of Meteorology and Water Management—National Research Institute. The Bai–Perron test was used to determine the direction of temperature changes. An AR(1) autoregression model was used to predict SAT changes in central Poland for the years 2022–2026, based on the results of the Bai–Perron test. As part of the in-depth research on the SAT index, reference evapotranspiration calculations were also made as a second factor that is considered an important indicator of climate change. The Sum of Active Temperatures from 1975 to 2021 in the provinces of central Poland showed an increasing trend of 0.07% per year. The average SAT in central Poland in 2022–2026 is expected to range from 2700 °C to 2760 °C. Considering the current thermal conditions in central Poland and the forecasts for the coming years, it can be expected that vineyard cultivation will develop in this region. However, the research shows that the observed increasing trend in evapotranspiration, both in total in individual years and in the period of the greatest vegetation, i.e., in the months from May to the end of August, will result in an increasing need in central Poland to ensure adequate irrigation in developing vineyards.
Behavior of Thiamethoxam and Clothianidin in Young Oilseed Rape Plants before Flowering, Monitored by QuEChERS/LC–MS/MS Protocol
Authors: Hrynko Izabela , Ilyasova Gulzhakhan , Jankowska Magdalena , Rutkowska Ewa , Kaczyński Piotr , Łozowicka Bożena
Published: May 2024

Nitro-substituted neonicotinoid insecticides have been widely used until recently to control a range of important agricultural pests. Growing concerns about thiamethoxam’s toxicity to pollinators have led to its use being restricted or to it even being banned in some countries. Nevertheless, in Asia, Africa, Southeast Europe, and South America thiamethoxam is still used. Although thiamethoxam has been intensively studied all over the world, its dissipation dynamics have not been studied in depth. The subject of the present study was to (1) develop and validate a QuEChERS/LC-MS/MS protocol for the determination of thiamethoxam and its main metabolite clothianidin in samples of young oilseed rape plants with high chlorophyll content, and (2) make a comparison of the degradation behaviors of thiamethoxam and clothianidin in two crops of winter oilseed rape, cultivated on soils with different pH. For determination of thiamethoxam and clothianidin in plant material with high chlorophyll content, a QuEChERS/LC–MS/MS protocol enabling the detection of low levels of compound concentrations was developed. The proposed clean-up protocol provided recoveries within the range of 92–98% for the compounds under analysis. Precision, calculated as relative standard deviation, was below 20%. Satisfactory linearity of the method was obtained in the concentration range under analysis (0.001–1.0 mg kg−1). Differences in degradation of both insecticides, depending on the physico-chemical properties of the soil, were observed. Thiamethoxam and clothianidin residues disappeared in plants very quickly, and they were not detected below the limit of quantitation in oilseed rape at the flowering stage.
The Inclusion of Pigeon Pea Hay Improves the Quality of Giant Cactus Harvested at Different Times
Authors: Saldanha Rodrigo B. , de Carvalho Gleidson G. P. , Rodrigues Carlindo S. , Silva Tarcizio V. B. S. , Pina Douglas dos S. , Alba Henry D. R. , Santos Stefanie A. , Tosto Manuela S. L. , Romão Claudio de O.
Published: May 2024

The objective of this study was to evaluate the effects of giant cactus harvest time and the inclusion of pigeon pea hay in improving the ensiling process and the nutritional composition of cactus silage. In total, 40 polyvinyl chloride-PVC mini-silos (10 × 40 cm) were used. Mini-silos were distributed in a 2 × 5 factorial scheme, referent to 2 harvest times (18 and 24 months) and 5 levels (0, 10%, 20%, 30%, and 40%) of pigeon pea hay inclusion in a randomized design. Effluent losses (p < 0.001) showed a negative linear effect as the inclusion of pigeon pea hay increased. Gas losses (p < 0.001), dry matter (DM) recovery (p < 0.001), and pH (p < 0.001) revealed the interactive effects between the pigeon pea hay inclusion and the harvest time. The DM content was higher in the giant cactus harvested at 24 months. The difference in nutritional composition promoted by the harvest time did not affect the silage quality. The gradual inclusion of pigeon pea hay improved the fermentative and bromatological characteristics of giant cactus silage. It is important to highlight that the inclusion of pigeon pea hay contributed to the increase in pH without affecting the necessary acidity for ideal conservation.
Evaluating Different Methods to Establish Biodiverse Swards of Native Grasses and Wildflowers for Pasturelands
Authors: Kubesch Jonathan O. C. , Greiner Scott P. , Pent Gabriel J. , Reid J. Leighton , Tracy Benjamin F.
Published: May 2024

Many cool-season pastures in the southeastern U.S. are dominated by a competitive cool-season grass, tall fescue (Schedonorus arundinaceus), and lack substantial plant diversity. Planting native warm-season grasses (NWSGs) and wildflowers (WFs) into these pastures could provide summer forage for cattle and more floral resources for pollinators. This paper summarizes field experiments designed to evaluate different spatiotemporal planting arrangements of NWSGs and WFs to improve their establishment success. The study was conducted from April 2021 to October 2023 in central Virginia (USA). Planting treatments included NWSG and WF mixtures planted: (1) together in the same space, (2) spatially separated in space (i.e., side by side), or (3) temporally separated where NWSGs and WFs were planted in difference sequences. Results showed few differences in forage mass, floral production, and botanical composition as well as stand density in 2021 and 2022. In 2023, NWSG abundance was greater where grasses were planted first or mixed with WFs. Similarly, the WF component was favored when they were planted before NWSGs. Overall, planting NWSG and WF mixes separately, either spatially or temporally, favors successful establishment and could offer more flexibility for using selective herbicides to suppress the heavy weed pressure that often accompanies these plantings.
YOLO-Based Phenotyping of Apple Blotch Disease (Diplocarpon coronariae) in Genetic Resources after Artificial Inoculation
Authors: Reim Stefanie , Richter Sophie , Leonhardt Oskar , Maß Virginia , Wöhner Thomas Wolfgang
Published: May 2024

Phenotyping of genetic resources is an important prerequisite for the selection of resistant varieties in breeding programs and research. Computer vision techniques have proven to be a useful tool for digital phenotyping of diseases of interest. One pathogen that is increasingly observed in Europe is Diplocarpon coronariae, which causes apple blotch disease. In this study, a high-throughput phenotyping method was established to evaluate genetic apple resources for susceptibility to D. coronariae. For this purpose, inoculation trials with D. coronariae were performed in a laboratory and images of infested leaves were taken 7, 9 and 13 days post inoculation. A pre-trained YOLOv5s model was chosen to establish the model, which was trained with an image dataset of 927 RGB images. The images had a size of 768 × 768 pixels and were divided into 738 annotated training images, 78 validation images and 111 background images without symptoms. The accuracy of symptom prediction with the trained model was 95%. These results indicate that our model can accurately and efficiently detect spots with acervuli on detached apple leaves. Object detection can therefore be used for digital phenotyping of detached leaf assays to assess the susceptibility to D. coronariae in a laboratory.
Ecological Civilisation and Amphibian Sustainability through Reproduction Biotechnologies, Biobanking, and Conservation Breeding Programs (RBCs)
Authors: Browne Robert K. , Luo Qinghua , Wang Pei , Mansour Nabil , Kaurova Svetlana A. , Gakhova Edith N. , Shishova Natalia V. , Uteshev Victor K. , Kramarova Ludmila I. , Venu Govindappa , Vaissi Somaye , Taheri-Khas Zeynab , Heshmatzad Pouria , Bagaturov Mikhail F. , Janzen Peter , Naranjo Renato E. , Swegen Aleona , Strand Julie , McGinnity Dale , Dunce Ilze
Published: May 2024

Intergenerational justice entitles the maximum retention of Earth’s biodiversity. The 2022 United Nations COP 15, “Ecological Civilisation: Building a Shared Future for All Life on Earth”, is committed to protecting 30% of Earth’s terrestrial environments and, through COP 28, to mitigate the effects of the climate catastrophe on the biosphere. We focused this review on three core themes: the need and potential of reproduction biotechnologies, biobanks, and conservation breeding programs (RBCs) to satisfy sustainability goals; the technical state and current application of RBCs; and how to achieve the future potentials of RBCs in a rapidly evolving environmental and cultural landscape. RBCs include the hormonal stimulation of reproduction, the collection and storage of sperm and oocytes, and artificial fertilisation. Emerging technologies promise the perpetuation of species solely from biobanked biomaterials stored for perpetuity. Despite significant global declines and extinctions of amphibians, and predictions of a disastrous future for most biodiversity, practical support for amphibian RBCs remains limited mainly to a few limited projects in wealthy Western countries. We discuss the potential of amphibian RBCs to perpetuate amphibian diversity and prevent extinctions within multipolar geopolitical, cultural, and economic frameworks. We argue that a democratic, globally inclusive organisation is needed to focus RBCs on regions with the highest amphibian diversity. Prioritisation should include regional and international collaborations, community engagement, and support for RBC facilities ranging from zoos and other institutions to those of private carers. We tabulate a standard terminology for field programs associated with RBCs for publication and media consistency.
Effects of Social Facilitation and Introduction Methods for Cattle on Virtual Fence Adaptation
Authors: Simonsen Pernille Arent , Husted Niels Søborg , Clausen Magnus , Spens Amalie-Maria , Dyrholm Rasmus Majland , Thaysen Ida Fabricius , Aaser Magnus Fjord , Staahltoft Søren Krabbe , Bruhn Dan , Alstrup Aage Kristian Olsen , Sonne Christian , Pertoldi Cino
Published: May 2024

Agricultural industries rely on physical fences to manage livestock. However, these present practical, financial, and ecological challenges, which may be solved using virtual fencing. This study aimed to identify how experienced cattle through social facilitation and the introduction method influence inexperienced cattle. Based on three stocks held in Fanø, Denmark, containing 12, 17 and 13 Angus (Bos taurus), we examined the virtual fence learning in three case studies using one gradual introduction with zero experienced cattle (A) and two different instant introductions with one (B) and ten (C) experienced cattle. Gradual introduction had the virtual fence moved 20 m every other day for eleven days, and in the two instant introductions, the physical fence was removed in one day. Warnings and impulses were recorded during an 11-day learning period and a 26-day post-learning period, using the impulses per warning to quantify if the cattle adapted. Case studies A and B showed a significant reduction in the warnings and impulses, but only A showed a significant reduction in the impulses per warning when comparing the learning period to the post-learning period. Due to the non-standardised experiments, it was not possible to conclude if the number of experienced cattle or the introduction method had an effect on the results.
Bovine Parainfluenza Virus 3 and Bovine Respiratory Syncytial Virus: Dominant Viral Players in Bovine Respiratory Disease Complex among Serbian Cattle
Authors: Milićević Vesna , Šolaja Sofija , Glišić Dimitrije , Ninković Milan , Milovanović Bojan , Đorđević Milan , Ristevski Snežana , Spasojević Filip , Dačić Miroljub
Published: May 2024

Bovine respiratory disease complex, a complex respiratory ailment in cattle, results from a combination of viral and bacterial factors, compounded by environmental stressors such as overcrowding, transportation, and adverse weather conditions. Its impact extends beyond mere health concerns, posing significant economic threats to the cattle industry. This study presents an extensive investigation into viral pathogens associated with BRDC in Serbian cattle, utilizing serum samples and nasal swabs. A cross-sectional study was conducted in 2024 across 65 randomly selected dairy farms in Serbia, excluding farms with vaccinated cattle. The farms were categorized by their livestock count: small (≤50 animals), medium (51–200 animals), and large (>200 animals). Serum samples from adult cattle older than 24 months were tested for antibodies against BVDV, BHV-1, BRSV, and BPIV3. Nasal swab samples from the animals with respiratory signs were tested using PCR for viral genome detection. The results showed seropositivity for all four viruses across all of the farms, with BPIV3 exhibiting universal seropositivity. Medium-sized and large farms demonstrated higher levels of seropositivity for BRSV and BHV-1 compared to small farms (p < 0.05). Our true seroprevalence estimates at the animal level were 84.29% for BRSV, 54.08% for BVDV, 90.61% for BHV-1, and 84.59% for BPIV3. A PCR analysis of the nasal swabs revealed positive detections for BRSV (20%), BHV-1 (1.7%), BVDV (8%), and BPIV3 (10.9%). Influenza D virus was not found in any of the samples. This study provides critical insights into the prevalence and circulation of viral pathogens associated with BRDC in Serbian cattle, emphasizing the importance of surveillance and control measures to mitigate the impact of respiratory diseases in cattle populations.
Humoral Immunity across the SARS-CoV-2 Spike after Sputnik V (Gam-COVID-Vac) Vaccination
Authors: Cornejo Alejandro , Franco Christopher , Rodriguez-Nuñez Mariajose , García Alexis , Belisario Inirida , Mayora Soriuska , Garzaro Domingo José , Zambrano José Luis , Jaspe Rossana Celeste , Hidalgo Mariana , Parra-Giménez Nereida , Claro Franklin Ennodio , Liprandi Ferdinando , de Waard Jacobus Henri , Rangel Héctor Rafael , Pujol Flor Helene
Published: May 2024

SARS-CoV-2 vaccines have contributed to attenuating the burden of the COVID-19 pandemic by promoting the development of effective immune responses, thus reducing the spread and severity of the pandemic. A clinical trial with the Sputnik-V vaccine was conducted in Venezuela from December 2020 to July 2021. The aim of this study was to explore the antibody reactivity of vaccinated individuals towards different regions of the spike protein (S). Neutralizing antibody (NAb) activity was assessed using a commercial surrogate assay, detecting NAbs against the receptor-binding domain (RBD), and a plaque reduction neutralization test. NAb levels were correlated with the reactivity of the antibodies to the spike regions over time. The presence of Abs against nucleoprotein was also determined to rule out the effect of exposure to the virus during the clinical trial in the serological response. A high serological reactivity was observed to S and specifically to S1 and the RBD. S2, although recognized with lower intensity by vaccinated individuals, was the subunit exhibiting the highest cross-reactivity in prepandemic sera. This study is in agreement with the high efficacy reported for the Sputnik V vaccine and shows that this vaccine is able to induce an immunity lasting for at least 180 days. The dissection of the Ab reactivity to different regions of S allowed us to identify the relevance of epitopes outside the RBD that are able to induce NAbs. This research may contribute to the understanding of vaccine immunity against SARS-CoV-2, which could contribute to the design of future vaccine strategies.
Inhibition of Candida albicans Biofilm Formation and Attenuation of Its Virulence by Liriope muscari
Authors: Lee Jeonghoon , Song Hyunchan , Kim Kiyoung
Published: May 2024

(1) Background: Although Candida albicans accounts for the majority of fungal infections, therapeutic options are limited and require alternative antifungal agents with new targets; (2) Methods: A biofilm formation assay with RPMI1640 medium was performed with Liriope muscari extract. A combination antifungal assay, dimorphic transition assay, and adhesion assay were performed under the biofilm formation condition to determine the anti-biofilm formation effect. qRT-PCR analysis was accomplished to confirm changes in gene expression; (3) Results: L. muscari extract significantly reduces biofilm formation by 51.65% at 1.56 μg/mL use and therefore increases susceptibility to miconazole. L. muscari extract also inhibited the dimorphic transition of Candida; nearly 50% of the transition was inhibited when 1.56 μg/mL of the extract was treated. The extract of L. muscari inhibited the expression of genes related to hyphal development and extracellular matrix of 34.4% and 36.0%, respectively, as well as genes within the Ras1-cAMP-PKA, Cph2-Tec1, and MAP kinase signaling pathways of 25.58%, 7.1% and 15.8%, respectively, at 1.56 μg/mL of L. muscari extract treatment; (4) Conclusions: L. muscari extract significantly reduced Candida biofilm formation, which lead to induced antifungal susceptibility to miconazole. It suggests that L. muscari extract is a promising anti-biofilm candidate of Candida albicans since the biofilm formation of Candida albicans is an excellent target for candidiasis regulation.
AMPs as Host-Directed Immunomodulatory Agents against Skin Infections Caused by Opportunistic Bacterial Pathogens
Authors: Saha Subhasree , Barik Devashish , Biswas Debabrata
Published: May 2024

Skin is the primary and largest protective organ of the human body. It produces a number of highly evolved arsenal of factors to counter the continuous assault of foreign materials and pathogens from the environment. One such potent factor is the repertoire of Antimicrobial Peptides (AMPs) that not only directly destroys invading pathogens, but also optimally modulate the immune functions of the body to counter the establishment and spread of infections. The canonical direct antimicrobial functions of these AMPs have been in focus for a long time to design principles for enhanced therapeutics, especially against the multi-drug resistant pathogens. However, in recent times the immunomodulatory functions performed by these peptides at sub-microbicidal concentrations have been a point of major focus in the field of host-directed therapeutics. Such strategies have the added benefit of not having the pathogens develop resistance against the immunomodulatory pathways, since the pathogens exploit these signaling pathways to obtain and survive within the host. Thus, this review summarizes the potent immunomodulatory effect of these AMPs on, specifically, the different host immune cells with the view of providing a platform of information that might help in designing studies to exploit and formulate effective host-directed adjunct therapeutic strategies that would synergies with drug regimens to counter the current diversity of drug-resistant skin opportunistic pathogens.
Antimicrobial Properties of Newly Developed Silver-Enriched Red Onion–Polymer Composites
Authors: Puišo Judita , Žvirgždas Jonas , Paškevičius Algimantas , Arslonova Shirin , Adlienė Diana
Published: May 2024

Simple low-cost, nontoxic, environmentally friendly plant-extract-based polymer films play an important role in their application in medicine, the food industry, and agriculture. The addition of silver nanoparticles to the composition of these films enhances their antimicrobial capabilities and makes them suitable for the treatment and prevention of infections. In this study, polymer-based gels and films (AgRonPVA) containing silver nanoparticles (AgNPs) were produced at room temperature from fresh red onion peel extract (“Ron”), silver nitrate, and polyvinyl alcohol (PVA). Silver nanoparticles were synthesized directly in a polymer matrix, which was irradiated by UV light. The presence of nanoparticles was approved by analyzing characteristic local surface plasmon resonance peaks occurring in UV-Vis absorbance spectra of irradiated experimental samples. The proof of evidence was supported by the results of XRD and EDX measurements. The diffusion-based method was applied to investigate the antimicrobial activity of several types of microbes located in the environment of the produced samples. Bacteria Staphylococcus aureus ATCC 29213, Acinetobacter baumannii ATCC BAA 747, and Pseudomonas aeruginosa ATCC 15442; yeasts Candida parapsilosis CBS 8836 and Candida albicans ATCC 90028; and microscopic fungi assays Aspergillus flavus BTL G-33 and Aspergillus fumigatus BTL G-38 were used in this investigation. The greatest effect was observed on Staphylococcus aureus, Acinetobacter baumannii, and Pseudomonas aeruginosa bacteria, defining these films as potential candidates for antimicrobial applications. The antimicrobial features of the films were less effective against fungi and the weakest against yeasts.
Oxidative Stress, Lipid Peroxidation and Ferroptosis Are Major Pathophysiological Signatures in the Placental Tissue of Women with Late-Onset Preeclampsia
Authors: Ortega Miguel A. , Garcia-Puente Luis M. , Fraile-Martinez Oscar , Pekarek Tatiana , García-Montero Cielo , Bujan Julia , Pekarek Leonel , Barrena-Blázquez Silvestra , Gragera Raquel , Rodríguez-Rojo Inmaculada C. , Rodríguez-Benitez Patrocinio , López-González Laura , Díaz-Pedrero Raul , Álvarez-Mon Melchor , García-Honduvilla Natalio , De León-Luis Juan A. , Bravo Coral , Saez Miguel A.
Published: May 2024

Preeclampsia, a serious and potentially life-threatening medical complication occurring during pregnancy, is characterized by hypertension and often accompanied by proteinuria and multiorgan dysfunction. It is classified into two subtypes based on the timing of diagnosis: early-onset (EO-PE) and late-onset preeclampsia (LO-PE). Despite being less severe and exhibiting distinct pathophysiological characteristics, LO-PE is more prevalent than EO-PE, although both conditions have a significant impact on placental health. Previous research indicates that different pathophysiological events within the placenta may contribute to the development of preeclampsia across multiple pathways. In our experimental study, we investigated markers of oxidative stress, ferroptosis, and lipid peroxidation pathways in placental tissue samples obtained from women with LO-PE (n = 68) compared to healthy control pregnant women (HC, n = 43). Through a comprehensive analysis, we observed an upregulation of specific molecules associated with these pathways, including NADPH oxidase 1 (NOX-1), NADPH oxidase 2 (NOX-2), transferrin receptor protein 1 (TFRC), arachidonate 5-lipoxygenase (ALOX-5), acyl-CoA synthetase long-chain family member 4 (ACSL-4), glutathione peroxidase 4 (GPX4) and malondialdehyde (MDA) in women with LO-PE. Furthermore, increased ferric tissue deposition (Fe3+) was observed in placenta samples stained with Perls’ Prussian blue. The assessment involved gene and protein expression analyses conducted through RT-qPCR experiments and immunohistochemistry assays. Our findings underscore the heightened activation of inflammatory pathways in LO-PE compared to HC, highlighting the pathological mechanisms underlying this pregnancy disorder.
Analysis of the Efficiency of Antioxidants in Inhibiting Lipid Oxidation in Terms of Characteristic Kinetic Parameters
Authors: Losada-Barreiro Sonia , Paiva-Martins Fátima , Bravo-Díaz Carlos
Published: May 2024

In this work, we aim to find physical evidence demonstrating the crucial role that the effective concentration of antioxidants (AOs) present at the interfacial region of emulsions has in controlling the inhibition of the lipid oxidation reaction. We prepared a series of antioxidants of different hydrophobicities derived from chlorogenic and protocatechuic acids. We first monitored, in intact emulsions, the (sigmoidal) production of conjugated dienes and determined the corresponding induction times, tind. Independently, we determined the effective concentrations of the antioxidants in the same intact emulsions. Results show that both the length of the induction periods and the antioxidant interfacial concentrations parallel each other, with a maximum at the octyl-dodecyl derivatives. The ratio between the interfacial antioxidant concentrations and the induction periods remains constant for all AOs in the same series, so that the rates of initiation of lipid oxidation are the same regardless of the hydrophobicity of the antioxidant employed. The constancy in the rate of initiation provides strong experimental evidence for a direct relationship between interfacial concentrations and antioxidant efficiencies. Results suggest new possibilities to investigate lipid peroxidation under non-forced conditions and are of interest to formulators interested in preparing emulsions with antimicrobial properties.
A Consolidated Understanding of the Contribution of Redox Dysregulation in the Development of Hearing Impairment
Authors: Yeo Xin Yi , Kwon Soohyun , Rinai Kimberley R. , Lee Sungsu , Jung Sangyong , Park Raekil
Published: May 2024

The etiology of hearing impairment is multifactorial, with contributions from both genetic and environmental factors. Although genetic studies have yielded valuable insights into the development and function of the auditory system, the contribution of gene products and their interaction with alternate environmental factors for the maintenance and development of auditory function requires further elaboration. In this review, we provide an overview of the current knowledge on the role of redox dysregulation as the converging factor between genetic and environmental factor-dependent development of hearing loss, with a focus on understanding the interaction of oxidative stress with the physical components of the peripheral auditory system in auditory disfunction. The potential involvement of molecular factors linked to auditory function in driving redox imbalance is an important promoter of the development of hearing loss over time.
The Antioxidant Dendrobium officinale Polysaccharide Modulates Host Metabolism and Gut Microbiota to Alleviate High-Fat Diet-Induced Atherosclerosis in ApoE−/− Mice
Authors: Qi Jingyi , Zhou Shuaishuai , Wang Guisheng , Hua Rongrong , Wang Xiaoping , He Jian , Wang Zi , Zhu Yinhua , Luo Junjie , Shi Wenbiao , Luo Yongting , Chen Xiaoxia
Published: May 2024

Background: The discovery of traditional plants’ medicinal and nutritional properties has opened up new avenues for developing pharmaceutical and dietary strategies to prevent atherosclerosis. However, the effect of the antioxidant Dendrobium officinale polysaccharide (DOP) on atherosclerosis is still not elucidated. Purpose: This study aims to investigate the inhibitory effect and the potential mechanism of DOP on high-fat diet-induced atherosclerosis in Apolipoprotein E knockout (ApoE−/−) mice. Study design and methods: The identification of DOP was measured by high-performance gel permeation chromatography (HPLC) and Fourier transform infrared spectroscopy (FTIR). We used high-fat diet (HFD)-induced atherosclerosis in ApoE−/− mice as an animal model. In the DOP intervention stage, the DOP group was treated by gavage with 200 μL of 200 mg/kg DOP at regular times each day and continued for eight weeks. We detected changes in serum lipid profiles, inflammatory factors, anti-inflammatory factors, and antioxidant capacity to investigate the effect of the DOP on host metabolism. We also determined microbial composition using 16S rRNA gene sequencing to investigate whether the DOP could improve the structure of the gut microbiota in atherosclerotic mice. Results: DOP effectively inhibited histopathological deterioration in atherosclerotic mice and significantly reduced serum lipid levels, inflammatory factors, and malondialdehyde (F/B) production. Additionally, the levels of anti-inflammatory factors and the activity of antioxidant enzymes, including superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX), were significantly increased after DOP intervention. Furthermore, we found that DOP restructures the gut microbiota composition by decreasing the Firmicutes/Bacteroidota (F/B) ratio. The Spearman’s correlation analysis indicated that serum lipid profiles, antioxidant activity, and pro-/anti-inflammatory factors were associated with Firmicutes, Bacteroidota, Allobaculum, and Coriobacteriaceae_UCG-002. Conclusions: This study suggests that DOP has the potential to be developed as a food prebiotic for the treatment of atherosclerosis in the future.
Harnessing Agri-Food Waste as a Source of Biopolymers for Agriculture
Authors: Valle Carlotta , Voss Monica , Calcio Gaudino Emanuela , Forte Claudio , Cravotto Giancarlo , Tabasso Silvia
Published: May 2024

Nowadays, the world is facing a general problem of resource overconsumption and waste overproduction: to address these two issues, the United Nations delivered the 12th Sustainable Development Goal (SDG), which has the objective of ensuring sustainable consumption and production patterns. Currently, polymers are present in every aspect of our lives and have the disadvantage of mostly coming from fossil sources and causing pollution when disposed of the wrong way. Agriculture plays a key role in the overall world environmental issues, being responsible for the creation of between 13 and 21% of global greenhouse gas (GHG) emissions. Moreover, it represents a continuously growing field, producing large amounts of waste. These residues can cause serious environmental concerns and high costs when disposed. However, agri-food waste (AFW) is a natural source of natural biopolymers, such as lignin, cellulose, pectin, and starch, but can also be used as a substrate to produce other non-toxic and biodegradable biopolymers, such as chitosan, polyhydroxyalkanoates (PHAs), and polylactic acid (PLA) through microbial fermentation. These polymers find applications in agricultural practices such as mulching films, soil stabilizers, hydrogels, nanocarriers, and coating for seeds, fruits, and vegetables. The employment of AFW in the production of non-toxic, sustainable, and biodegradable biopolymers for their agricultural utilization is an example of a virtuous circular economy approach that could help agriculture to be more sustainable.
Texture-Guided Graph Transform Optimization for Point Cloud Attribute Compression
Authors: Shao Yiting , Song Fei , Gao Wei , Liu Shan , Li Ge
Published: May 2024

There is a pressing need across various applications for efficiently compressing point clouds. While the Moving Picture Experts Group introduced the geometry-based point cloud compression (G-PCC) standard, its attribute compression scheme falls short of eliminating signal frequency-domain redundancy. This paper proposes a texture-guided graph transform optimization scheme for point cloud attribute compression. We formulate the attribute transform coding task as a graph optimization problem, considering both the decorrelation capability of the graph transform and the sparsity of the optimized graph within a tailored joint optimization framework. First, the point cloud is reorganized and segmented into local clusters using a Hilbert-based scheme, enhancing spatial correlation preservation. Second, the inter-cluster attribute prediction and intra-cluster prediction are conducted on local clusters to remove spatial redundancy and extract texture priors. Third, the underlying graph structure in each cluster is constructed in a joint rate–distortion–sparsity optimization process, guided by geometry structure and texture priors to achieve optimal coding performance. Finally, point cloud attributes are efficiently compressed with the optimized graph transform. Experimental results show the proposed scheme outperforms the state of the art with significant BD-BR gains, surpassing G-PCC by 31.02%, 30.71%, and 32.14% in BD-BR gains for Y, U, and V components, respectively. Subjective evaluation of the attribute reconstruction quality further validates the superiority of our scheme.
Designing a Competency-Focused Course on Applied AI Based on Advanced System Research on Business Requirements
Authors: Martsenyuk Vasyl , Dimitrov Georgi , Rancic Dejan , Luptakova Iveta Dirgova , Jovancevic Igor , Bernas Marcin , Klos-Witkowska Aleksandra , Gancarczyk Tomasz , Kostadinova Iva , Mihaylova Elizabet , Stojanovic Dragan , Milojkovic Marko , Pospichal Jiri , Plamenac Aleksandar
Published: May 2024

The consortium of “The Future is in Applied Artificial Intelligence” Project designed the first competency-based applied artificial intelligence curriculum at the higher-education institution level. The development was based on advanced system research on existing artificial intelligence-related resources and surveying target groups of teachers, information technology students, and employers, which should enhance the performance of implementing artificial intelligence education. A review of applied artificial intelligence was prepared in the form of keyword clustering. The initial data were collected with the help of surveying by identifying job offers, existing artificial intelligence training courses, scientific projects, and real cases. A synthetic analysis of the textual information from the studies was conducted using the word clouds technique. A tensor-based approach was used for the presentation of the competency-based course. The specific numerical requirements for the course in the form of priorities followed from the solution to decision-making problems using the analytic hierarchy process technique. Based on a comprehensive study of surveys, educational experience, scientific projects, and business requirements, and a meta-analysis of the recent references, we specified the criteria for a training course in the form of a tensor-based representation of competencies in relation to content and educational modules.
Evolution of Core Stability, Athletic Performance, and ACL Injury Risk across a Soccer Season
Authors: Weber Théo A. , Duchene Youri , Simon Frédéric R. , Mornieux Guillaume , Gauchard Gerôme C.
Published: May 2024

Soccer athletic performance varies across a soccer season due to training and fatigue. In addition, it is known that core stability is linked with anterior cruciate ligament (ACL) injury risk but their variations over a season are unknown. The aim of the study was to determine the evolution of core stability, athletic performance, and ACL injury risk among young high-level soccer players at four key moments of a season: pre-season (PRE), start of season (START), mid-season (MID), and the end of the season (END). Core stability scores increased until mid-season, while ACL injury risk scores (measured during sidestep cuttings and single-leg landing) decreased thanks to an injury prevention program between START and MID. These results are in line with the literature, which demonstrates that a high level of core stability is linked to a low injury risk. Evolution of athletic performance was not consistent throughout the season, being dependent on the specific phases of training performed by the athletes. Therefore, assessing core stability, athletic performance, and ACL injury risk multiple times across a soccer season could help coaches to adapt their training programs properly.
Reviewing CAM-Based Deep Explainable Methods in Healthcare
Authors: Tang Dan , Chen Jinjing , Ren Lijuan , Wang Xie , Li Daiwei , Zhang Haiqing
Published: May 2024

The use of artificial intelligence within the healthcare sector is consistently growing. However, the majority of deep learning-based AI systems are of a black box nature, causing these systems to suffer from a lack of transparency and credibility. Due to the widespread adoption of medical imaging for diagnostic purposes, the healthcare industry frequently relies on methods that provide visual explanations, enhancing interpretability. Existing research has summarized and explored the usage of visual explanation methods in the healthcare domain, providing introductions to the methods that have been employed. However, existing reviews are frequently used for interpretable analysis in the medical field ignoring comprehensive reviews on Class Activation Mapping (CAM) methods because researchers typically categorize CAM under the broader umbrella of visual explanations without delving into specific applications in the healthcare sector. Therefore, this study primarily aims to analyze the specific applications of CAM-based deep explainable methods in the healthcare industry, following the PICO (Population, Intervention, Comparison, Outcome) framework. Specifically, we selected 45 articles for systematic review and comparative analysis from three databases—PubMed, Science Direct, and Web of Science—and then compared eight advanced CAM-based methods using five datasets to assist in method selection. Finally, we summarized current hotspots and future challenges in the application of CAM in the healthcare field.
Research on Bus Scheduling Optimization Considering Exhaust Emission Based on Genetic Algorithm: Taking a Route in Nanjing City as an Example
Authors: Wang Meixia , Guo Baohua , Zhang Zhezhe , Zhang Yanshuang
Published: May 2024

In order to enhance passenger willingness to choose buses for commuting and to reduce the operating costs and tailpipe emissions of bus companies, a bus scheduling model is established. The model aims to minimize the sum of the operating costs of the bus company, the costs associated with the loss of passengers’ interest, and the cost of tailpipe emissions. It considers constraints such as maintaining an average load factor of the buses above 60%, ensuring a departure interval of greater than 5 min during non-peak hours and less than 30 min during peak hours, and limiting the maximum number of buses allocated to a route. The passenger flow is divided into peak hours and nonpeak hours according to the survey of passenger flow during each period of a bus operation on a route in Nanjing City, China. A genetic algorithm is employed to solve the proposed bus scheduling model and determine the total costs during peak and non-peak hours. After designing the parameters of the genetic algorithm, optimal departure intervals and bus numbers for a day’s operation cycle on a given route are calculated using a weighting method.
Surface Ripple Formation by Bombardment with Clusters: Influence of Mass
Authors: Jiménez-Sáez José Carlos , Muñoz Sagrario , Palacios Pablo
Published: May 2024

Nanostructure formation on Co(110) surfaces was studied by using irradiation with cluster ion beams with oblique incidence and an energy of 250 eV/atom. In this work, the effect of the mass of the cluster projectiles on the process was analyzed. The launched clusters were formed by different types of charged atoms: He, Ne, Ar, Kr, and Xe. Due to the different collision processes, the formed surface patterns stand out more if the mass of the projectile atoms is greater, regardless of the angle of incidence of the clusters. Two processes control the morphological evolution of the surface during the bombardment phase: sputtering erosion and surface atomic redistribution. At grazing angles, the contribution of sputtering is greater during the process. In fact, heavier species give greater sputtering, and the redistribution factor becomes lower. The weight of redistribution is greater for intermediate angles above the critical angle (50° and 60°), since the displacement is greater for heavier species, and the redistribution factor takes substantially higher values. The experimental results point to a shift in the critical angle with the mass of the projectile atom. In the case of He, a very light ion, the results are marked by channeling and vertical displacements.
Cooling and Multiphase Analysis of Heated Environmentally Friendly R152A (C2H4F2) Fluid Coming from the Production Process According to Nist Indicators
Authors: Kartal Mehmet Akif , Atakök Gürcan , Ersoy Sezgin
Published: May 2024

Cooling processes are responsible for a significant portion (20%) of global energy consumption and raise environmental concerns such as ozone depletion, the greenhouse effect, and high energy use. This study investigates the potential of R152a, a refrigerant with low global warming potential (GWP), as a more sustainable alternative. The performance, safety, and operational efficiency of R152a were evaluated under various conditions. Although R152a offers high performance and low GWP, its flammability necessitates caution, especially in certain mixtures. A 12-pass tube-type heat exchanger model was simulated using computational fluid dynamics (CFD) to analyze the fluid behavior within the exchanger. The pressure, density, dynamic pressure, Prandtl number, total pressure, and temperature distributions for both R152a and H2O (water) were visualized using contour plots. The simulations comprehensively examined the fluid behavior inside and outside the heat exchanger. The results revealed the influence of the temperature on the internal dynamic pressure and density of R152a. Compared with R134a, R152a demonstrated superior performance but a lower coefficient of performance (COP) than R32. Studies also suggest that R152a exhibits lower irreversibility in Organic Rankine Cycle (ORC) systems than R245fa. These findings suggest that R152a holds promise for future refrigeration systems, as supported by existing research on its performance and compatibility. One study focused on optimizing the heat exchanger performance by maximizing the heat capacity and minimizing the pressure drop. This study employed a parallel-flow heat exchanger with R152a as the coolant for the hot process water. The temperature changes, pressure drops, and resulting energy efficiency and thermal performance of both fluids were analyzed. The results highlight the distinct energy efficiencies and thermal performance of the employed fluids.