bluuebunny/crossref_metadata_2025 huggingface dataset:
The Role of the Inhibitory Ligand HVEM and Its Receptors CD160 and BTLA in the Regulation of Anti-retroviral T Cell Responses
Authors: David Paul , Westmeier Jaana , Drabczyk-Pluta Malgorzata , Werner Tanja , Ickler Julia , Francois Sandra , Bayer Wibke , Sutter Kathrin , Del Rio Maria Luisa , Rodriguez-Barbosa Jose-Ignacio , Dittmer Ulf , Zelinskyy Gennadiy
Published: March 2022

Specific CD8+ T cells are crucial for the control of viruses. However, during many chronic viral infections these cells become dysfunctional. Immune checkpoint receptors, like PD-1 expressed on CD8+ T cells, contribute to this functional suppression during chronic infection. However, during the acute phase of infection virus-specific CD8+ T cells express high levels of PD-1 but are fully competent in killing virus-infected cells and there is increasing evidence that the biological activity of inhibitory receptors is strongly influenced by the availability of their respective ligands. We determined the expression of ligands for inhibitory receptors on infected myeloid cells during the acute phase of Friend retroviral (FV) infection. FV infection of granulocytes, monocytes, and macrophages strongly increased the cell surface expression of PD-L1 and the recently described ligand HVEM for inhibitory receptors BTLA and CD160. In addition, the infection of human myeloid cells in vitro with HIV also enhanced the expression of PD-L1 and HVEM. In infected mice, the upregulation of inhibitory ligands on infected cells was accompanied by enhanced frequencies of FV-specific CD8+ T cells that express PD-1, and the inhibitory receptors CD160 and BTLA. To define the functional effects of HVEM on activated CD8+ T cells, FV-infected mice were treated with blocking antibodies that prevented the interaction of HVEM with its two receptors, CD160 or BTLA, alone or in combination with anti-PD-L1 antibodies. Blocking the interaction of HVEM with CD160 and BTLA improved the production of cytotoxic molecules and the elimination of FV-infected cells. This effect was augmented when the therapy was combined with anti-PD-L1 antibodies, resulting in an additional expansion of cytotoxic CD8+ T cells. Thus, the ligand HVEM for the inhibitory receptors CD160 and BTLA downregulates the functionality of CD8+ T cells during retroviral infection and are potential targets for the immunomodulatory therapy of chronic viral infections.
Transcriptome Analysis of Lungs in a Mouse Model of Severe COVID-19
Authors: Cohen-Gihon Inbar , Israeli Ofir , Tidhar Avital , Sapoznikov Anita , Evgy Yentl , Stein Dana , Aftalion Moshe , Gur David , Orr Irit , Zvi Anat , Sabo Tamar , Kronman Chanoch , Falach Reut
Published: March 2022

Severe manifestations of coronavirus disease 2019 (COVID-19) are mostly restricted to distinct groups of people who have preexisting morbidities. Most COVID-19 animal models develop a mild pathology that resolves within a relatively short period of time, reflecting the more prevalent asymptomatic-to-mild performance of the disease observed in humans. Mice are normally unaffected by SARS coronavirus-2 infection, because of the inability of the virus to bind effectively to the murine angiotensin-converting enzyme 2 (ACE2) receptor. We have previously demonstrated that induction of mild and transient pulmonary morbidity, by application of low doses of ricin, rendered CD1 mice to be susceptible to this virus, which was displayed by sustained body weight loss and mortality rates >50%. In the present study, we performed transcriptomic analyses charting the major alterations in gene expression of mice that were pre-exposed to low doses of ricin and then subjected to SARS-CoV-2 infection compared to mice that were solely exposed to ricin or infected with SARS-CoV-2. Mice intoxicated and infected with ricin and SARS-CoV-2 demonstrated a marked stimulation of essential immunity genes and biological pathways involved in the activation of natural-killer response, cell death receptors, cytotoxic T-cells, Toll-like receptor signaling and the NLRP3 inflammasome pathway. At the protein level, an induced early and transient interferon response was recorded which was subsequently suppressed. The activation of this array of genes predicts clinical manifestations that are consistent with severe COVID-19 in humans, thereby establishing the suitability of this unique animal model for the study of severe COVID-19 disease.
Management Zones in Pastures Based on Soil Apparent Electrical Conductivity and Altitude: NDVI, Soil and Biomass Sampling Validation
Authors: Serrano João , Shahidian Shakib , Paixão Luís , Marques da Silva José , Moral Francisco
Published: March 2022

The intensification of the Montado mixed ecosystem (agro–silvo–pastoral) is a current endeavor in the context of promoting the sustainability of extensive livestock production in the Mediterranean region. Increased pasture productivity and extensive animal production involves the use of technologies to monitor spatial variability and to implement differentiated management of pasture grazing, fertilization or soil amendment. An intermediate step should lead to the identification and demarcation of areas with similar characteristics (soil and/or crop development), known as homogeneous management zones (HMZ) to implement site-specific management strategies. In this study, soil apparent electrical conductivity (ECa) and altimetry surveys were carried out in six experimental pasture fields with a non-contact electromagnetic induction sensor (EM38) associated with a Global Navigation Satellite System (GNSS) receiver. These ECa and topographic maps were used in geostatistical analyses for designing and establishing final classification maps with three HMZ (less, intermediate and more potential). The normalized difference vegetation index (NDVI), obtained from a proximal optical sensor, and soil and biomass sampling were used to validate these HMZ. From a practical perspective, these HMZ are the basis for preparation of fertilizer prescription maps and use of variable rate technology (VRT) in a Precision Agriculture project.
Torsion Test vs. Other Methods to Obtain the Shear Strength of Elastic-Plastic Adhesives
Authors: Ferraris Monica , Salvo Milena , Casalegno Valentina , De La Pierre Stefano , Goglio Luca , Benelli Alessandro
Published: March 2022

Nowadays adhesive joints are more and more used; therefore, a precise and reliable shear strength measurement of these joints is necessary to design and predict a final components’ performance. This work aimed to assess the shear strength value of adhesively joined ceramics (SiC, Si3N4) and steel in the case of an elasto-plastic (ductile) joining material (Loctite EA 9321 AERO) by an experimental campaign and associated analytical modelling. The joined samples were tested using a single lap offset test in compression (SLO), an asymmetrical 4-point bending test (A4PB, ASTM C1469), and by torsion on fully joined hourglass shaped samples (THG). A simple model based on the elastic-plastic response in shear was proposed to fit the torque-rotation curve measured in the torsion tests. The results showed that, with the adopted test methods and conditions, and by using the model, consistent values of shear strength could be obtained by torsion tests.
Air Quality Impact Assessment of a Waste-to-Energy Plant: Modelling Results vs. Monitored Data
Authors: Lonati Giovanni , Cernuschi Stefano , Giani Paolo
Published: March 2022

The impact of the emissions from a municipal Waste-to-Energy (WtE) plant in Northern Italy on local air quality was assessed using the CALMET-CALPUFF atmospheric dispersion modelling system. Model simulations were based on hourly emission rates measured by continuous stack monitoring systems and considered both air quality-regulated pollutants (nitrogen oxides, particulate matter, toxic elements, benzo(a)pyrene), and other trace pollutants typical of WtE plants (dioxins, furans, and mercury). The model results were compared to both long-term observations from the air quality monitoring network and with short-term measurements from dedicated monitoring campaigns in the vicinity of the WtE plant, in both warm and cold season conditions. Modelling and observational results showed that the estimated plant contributions are very limited. This suggests that the observed concentration levels were the result of the contribution of all the sources distributed over the area and that they were not solely driven by the activity of the plant. Estimated contributions from the plant’s emissions were usually at least two orders of magnitudes lower than the ambient levels at the nearest monitoring site and even lower at the farthest sites.
Targeting circDGKD Intercepts TKI’s Effects on Up-Regulation of Estrogen Receptor β and Vasculogenic Mimicry in Renal Cell Carcinoma
Authors: Ding Jie , Cui Xin-Gang , Chen Hao-Jie , Sun Yin , Yu Wei-Wei , Luo Jie , Xiao Guang-Qian , Chang Chawnshang , Qi Jun , Yeh Shuyuan
Published: March 2022

Vasculogenic mimicry (VM) has been reported as an alternative channel to increase tumor nutrient supplies and accelerate tumor progression, and is associated with poor survival prognosis in multiple cancers, including renal cell carcinoma (RCC). The currently used anti-angiogenic treatment for metastatic RCC, sunitinib, a tyrosine kinase inhibitor (TKI), has been reported to induce VM formation. Previously we identified that the estrogen receptor β (ERβ) functions as an oncogenic factor to promote RCC progression, supported by the analytic results from The Cancer Genome Atlas (TCGA) database. We have also found evidence that sunitinib induces RCC VM formation by up-regulating ERβ expression. In this study, we further demonstrated that treatment with sunitinib, as well as axitinib, another TKI, could induce ERβ expression in RCC cell lines. Clinical clear cell RCC (ccRCC) patients with higher ERβ expression are more likely to be found VE-cadherin positive and VM positive. Mechanism dissection showed that TKI- induced ERβ transcriptionally up-regulates the circular RNA of DGKD (circDGKD, hsa_circ_0058763), which enhances VE-cadherin expression by sponging the microRNA miR-125-5p family. Targeting circDGKD intercepts sunitinib-pretreatment-induced RCC VM formation, reduces metastases and improves survival in an experimental orthotopic animal model. Targeting ERβ/circDGKD signals may improve the TKI efficacy and provide novel combination therapies for metastatic RCC.
Cerium-Copper Oxides Synthesized in a Multi-Inlet Vortex Reactor as Effective Nanocatalysts for CO and Ethene Oxidation Reactions
Authors: Dosa Melodj , Marin-Figueredo Miguel Jose , Sartoretti Enrico , Novara Chiara , Giorgis Fabrizio , Bensaid Samir , Fino Debora , Russo Nunzio , Piumetti Marco
Published: March 2022

In this study, a set of CuCeOx catalysts was prepared via the coprecipitation method using a Multi-Inlet Vortex Reactor: the Cu wt.% content is 5, 10, 20, 30 and 60. Moreover, pure CeO2 and CuO were synthesized for comparison purposes. The physico-chemical properties of this set of samples were investigated by complementary techniques, e.g., XRD, N2 physisorption at −196 °C, Scanning Electron Microscopy, XPS, FT-IR, Raman spectroscopy and H2-TPR. Then, the CuCeOx catalysts were tested for the CO and ethene oxidation reactions. As a whole, all the prepared samples presented good catalytic performances towards the CO oxidation reaction (1000 ppm CO, 10 vol.% O2/N2): the most promising catalyst was the 20%CuCeOx (complete CO conversion at 125 °C), which exhibited a long-term thermal stability. Similarly, the oxidative activity of the catalysts were evaluated using a gaseous mixture containing 500 ppm C2H4, 10 vol.% O2/N2. Accordingly, for the ethene oxidation reaction, the 20%CuCeOx catalyst evidenced the best catalytic properties. The elevated catalytic activity towards CO and ethene oxidation was mainly ascribed to synergistic interactions between CeO2 and CuO phases, as well as to the high amount of surface-chemisorbed oxygen species and structural defects.
Deposition of Size-Selected Gold Nanoclusters onto Strontium Titanate Crystals for Water Splitting under Visible Light
Authors: Zhang Chuhang
Published: March 2022

Using a modulated pulse power magnetron sputtering (MPP-MSP) coupled with a quadrupole mass spectrometer (Q-MS), intensive size-selected gold nanoclusters (Aun) ranging from n = 5 to 40 in size are synthesized and soft landed onto a strontium titanate (STO) crystal surface as a co-catalyst for photocatalytic water splitting. The photocatalytic reactivity of the Aun/STO is investigated by measuring the photocurrent density of the sample under visible light radiation. It is found that the Aun co-catalysts enable the visible light response of the Aun/STO photocatalyst. The photocurrent density is sensitively dependent on the size of the Aun on the STO, and Au16 exhibits its maximum photocurrent under visible light. The underlying physics of the size-specific photocurrent are explained in terms of the size-dependent electron affinity of Aun.
Implanting MnO2 into Hexagonal Boron Nitride as Nanoadditives for Enhancing Tribological Performance
Authors: Chen Xiaorong , Chen Wenting , Zhang Linyi , Zeng Minli , Yang Shiming , Yang Zanhe , Ola Oluwafunmilola , Thummavichai Kunyapat , Wang Nannan , Zhu Yanqiu
Published: March 2022

Hexagonal boron nitride nanosheets (h-BNNs) show great potential in the field of tribology due to their typical two-dimensional layered structure and are essential for replacing conventional sulfur/phosphate-containing additives. However, the large particle size and poor dispersion of h-BNs seriously restrict their green lubrication application. In this paper, MnO2@h-BNNs nanocomposites were successfully prepared by ultrasonically exfoliating a hydrothermal method. The tribological properties of MnO2@h-BNNs nanocomposites as lubricant additives in poly-alpha-olefin oil (PAO) were investigated. The results show the oil dispersed with 0.25 wt% MnO2@h-BNNs had the best friction reduction and antiwear effect with 42% and 11.2% reduction, respectively, compared with the plain oil. Through further wear surface analyzing, we verified the antiwear mechanism of additives in filling the micropits and grooves on the wear surface and forming a friction protection film including Fe2O3, MnO2, and BN on the wear surface, avoiding direct contact between the friction subsets. This can provide ideas for other lubricating oil additives.
Fuzzy Based Backstepping Control Design for Stabilizing an Underactuated Quadrotor Craft under Unmodelled Dynamic Factors
Authors: Abro Ghulam E. Mustafa , Zulkifli Saiful Azrin B. M. , Ali Zain Anwar , Asirvadam Vijanth Sagayan , Chowdhry Bhawani Shankar
Published: March 2022

Since the quadrotor unmanned aerial vehicle (UAV) is one of the systems that has four (4) control inputs and six (6) degree of freedom (DOF) which makes it as an underactuated system. Such underactuated mechatronic systems are very difficult to stabilize but at the same time these systems are power efficient and cost-effective because of a lower number of actuators. Later, if someone tries to stabilize this underactuated quadrotor UAV under the impact of unmodelled dynamic factors, it will lead to huge instability, low convergence rate, chattering effect, trajectory deviation and may also encounter some of the serious transient and steady state issues as well. This paper presents one of the adaptive-robust control algorithms, called the fuzzy based backstepping control (FBSC) design, to address the quadrotor’s helical trajectory tracking issue under an influence of unmodelled dynamic factors and external disturbances. This manuscript proposes the synthesis of the proposed FBSC design using MATLAB and Simulink software whereas these results are correlated with the conventional backstepping control (BSC) algorithm to show the effectiveness of the proposed algorithm by computing the integral absolute error values with and without disturbances.
Growth Rates of Non-Native Bighead and Silver Carp in the Upper Mississippi River
Authors: Broaddus Cortney Lynn , Lamer James T.
Published: March 2022

Fish age and growth analyses can be used to infer spawning success, recruitment, and population age structure. Understanding these dynamics is important when assessing the impacts of invasive species, such as bighead carp, Hypophthalmichthys nobilis, and silver carp, H. molitrix, (bigheaded carp). These species have established throughout much of the Mississippi River Basin; however, Lock and Dam 19 (LD19) has slowed their establishment in the Upper Mississippi River, resulting in low-density populations with limited reproduction upstream. The age and growth of Bigheaded Carp in recently established populations, especially in this region, are poorly characterized. Therefore, we compared the age and growth of bigheaded carp for four low-density navigation pools of the Mississippi River near the invasion front with one long-established high-density population, separated by LD19. To assess growth rates and age at maturity, we estimated consensus age from pectoral spines, postcleithra, and vertebrae from 1229 bigheaded carp, 30 fish per 50 mm size class. Length at age of capture and back-calculated length-at-age were used to create growth curves using VBGC analysis. The analysis indicated higher growth rates of bighead carp and silver carp upstream of Lock and Dam 19 than downstream. Comparison of growth models with a likelihood ratio test indicated differences in length at time zero between the pools for bighead carp but not for silver carp. However, maximum length and growth rates were significantly different for silver carp upstream and downstream of the dam but not for bighead carp. These results will be used to better understand the population dynamics of this emerging population to inform control and containment actions.
Characteristics and Mechanism of Crayfish Myofibril Protein Gel Deterioration Induced by Autoclaving
Authors: Kang Xu , Ma Meihu , Yuan Jianglan , Huang Yaming
Published: March 2022

Crayfish myofibril protein (CMP) gel deterioration induced by autoclaving was investigated. A series of CMP gels were obtained through treating CMP solutions at different autoclaving conditions from 100 °C/0.1 MPa to 121 °C/0.21 MPa, and then characteristics and the mechanism of gel texture deterioration along with the intensification of autoclaving were explored through determining appearance, texture, protein composition, cross-linking forces, degree of hydrolysis, water state, microstructure of the gels, and average particle size of aggregates. When autoclaving was at above 105 °C/0.103 MPa, texture of CMP gel showed a tendency to severely weaken with the intensification of autoclaving (p < 0.05), hydrophobic interaction and aggregation between proteins weakened gradually (p < 0.05), and moderately bound water in the gel decreased and T22 relaxation time significantly increased (p < 0.05). After heating for 30 min at above 105 °C/0.103 MPa, pores in the microstructure of CMP gel enlarged obviously, and myosin heavy chain (MHC) degraded. It can be concluded that CMP gel deterioration induced by autoclaving was associated with the degradation of MHC and 105 °C might be the critical temperature to ensure good texture of crayfish products.
A Performance Evaluation Matrix for Measuring the Life Satisfaction of Older Adults Using eHealth Wearables
Authors: Jeng Mei-Yuan , Yeh Tsu-Ming , Pai Fan-Yun
Published: March 2022

eHealth wearables can track users’ state of health, record their physiological data, and facilitate self-care. In this study, we examined whether they enhance older adults’ casual exercise willingness and life satisfaction. After reviewing the related literature, the performance and satisfaction of elements for older adults to use eHealth Wearables were determined. The elements were derived from the means–end chain analysis. Three dimensions, product attributes, beneficial consequences, personal values, and responding elements, were identified first. The Performance Evaluation Matrix (PEM) was then established to determine the elements to be improved. A total of 250 questionnaires were distributed, out of which 206 valid questionnaires were completed and returned. In the proposed PEM, the product attributes that were in the priority improvement zone were accessibility, learnability, usability, affordability, positioning, pedometer, heart rate monitor, and data feedback. These elements are the most essential properties in need of improvement.
Association between Altitude and the Framingham Risk Score: A Cross-Sectional Study in the Peruvian Adult Population
Authors: Hernández-Vásquez Akram , Vargas-Fernández Rodrigo , Chacón-Diaz Manuel
Published: March 2022

To determine the association between altitude and the Framingham risk score in the Peruvian population, we performed a cross-sectional analytical study of data collected by the 2017–2018 Food and Nutrition Surveillance by Life Stages survey. The outcome of this study was the Framingham 10-year cardiovascular disease event risk prediction, which is composed of six modifiable and non-modifiable coronary risk factors. A generalized linear model (GLM) of the gamma family and log link function was used to report the crude and adjusted β coefficients. Several sensitivity analyses were performed to assess the association of interest. Data from a total of 833 surveyed participants were included. After adjusting for educational level, poverty level, alcohol consumption, physical activity level, the presence of any limitation, obesity, and area of residence, it was observed that altitude ≥ 2500 m above sea level (β = −0.42 [95% CI: −0.69 to −0.16]) was negatively and significantly associated with a decrease in the Framingham 10-year risk score. High altitude was significantly and negatively associated with Framingham 10-year risk scores. Our results will allow prevention strategies considering modifiable risk factors to avoid the development of cardiovascular diseases, especially in people living at low altitudes.
Resemblance-Ranking Peptide Library to Screen for Binders to Antibodies on a Peptidomic Scale
Authors: Jenne Felix , Biniaminov Sergey , Biniaminov Nathalie , Marquardt Philipp , von Bojničić-Kninski Clemens , Popov Roman , Seckinger Anja , Hose Dirk , Nesterov-Mueller Alexander
Published: March 2022

A novel resemblance-ranking peptide library with 160,000 10-meric peptides was designed to search for selective binders to antibodies. The resemblance-ranking principle enabled the selection of sequences that are most similar to the human peptidome. The library was synthesized with ultra-high-density peptide arrays. As proof of principle, screens for selective binders were performed for the therapeutic anti-CD20 antibody rituximab. Several features in the amino acid composition of antibody-binding peptides were identified. The selective affinity of rituximab increased with an increase in the number of hydrophobic amino acids in a peptide, mainly tryptophan and phenylalanine, while a total charge of the peptide remained relatively small. Peptides with a higher affinity exhibited a lower sum helix propensity. For the 30 strongest peptide binders, a substitutional analysis was performed to determine dissociation constants and the invariant amino acids for binding to rituximab. The strongest selective peptides had a dissociation constant in the hundreds of the nano-molar range. The substitutional analysis revealed a specific hydrophobic epitope for rituximab. To show that conformational binders can, in principle, be detected in array format, cyclic peptide substitutions that are similar to the target of rituximab were investigated. Since the specific binders selected via the resemblance-ranking peptide library were based on the hydrophobic interactions that are widespread in the world of biomolecules, the library can be used to screen for potential linear epitopes that may provide information about the cross-reactivity of antibodies.
Cleavage of Early Mouse Embryo with Damaged DNA
Authors: Baran Vladimír , Ďuríček Tomáš , Pisko Jozef , Drutovič Dávid , Šolc Petr
Published: March 2022

The preimplantation period of embryogenesis is crucial during mammalian ontogenesis. During this period, the mitotic cycles are initiated, the embryonic genome is activated, and the primary differentiation of embryonic cells occurs. All cellular abnormalities occurring in this period are the primary cause of fetal developmental disorders. DNA damage is a serious cause of developmental failure. In the context of DNA damage response on the cellular level, we analyzed the course of embryogenesis and phenotypic changes during the cleavage of a preimplantation embryo. Our results document that DNA damage induced before the resumption of DNA synthesis in a zygote can significantly affect the preimplantation development of the embryo. This developmental ability is related to the level of the DNA damage. We showed that one-cell embryos can correct the first cleavage cycle despite low DNA damage and incomplete replication. It seems that the phenomenon creates a predisposition to a segregation disorder of condensed chromatin that results in the formation of micronuclei in the developmental stages following the first cleavage. We conclude that zygote tolerates a certain degree of DNA damage and considers its priority to complete the first cleavage stage and continue embryogenesis as far as possible.
Parafibromin Is Highly Expressed in Hepatocellular Carcinoma and Its Expression Correlates with Poor Prognosis
Authors: Kim Min-Kyung
Published: March 2022

Hepatocellular carcinoma (HCC) is the second leading cause of cancer-related death worldwide. Much progress has been made regarding the understanding of hepatocarcinogenesis, yet the long-term survival rate of HCC patients remains poor. Recent efforts have shown parafibromin has a pathologic role in many human cancers, but little is known about the effects of parafibromin in HCC. This study aimed to investigate the pattern of parafibromin expression and its clinicopathologic significance in human HCC. Immunohistochemical analysis of HCC and matched non-tumor liver tissues from 50 HCC patients showed that the nuclear expression of parafibromin was higher in HCC tissues (50/50 cases) than in non-tumor liver tissues (17/50 cases). Moreover, elevated parafibromin expression was found to be significantly correlated with the presence of microvascular invasion (p = 0.017), hepatitis virus infection-induced occurrence (p = 0.005), and poorer tumor differentiation (Edmondson–Steiner grade; p = 0.000). Kaplan–Meier analysis showed that HCC patients with elevated parafibromin expression had poorer recurrence-free (p = 0.014, log-rank test = 6.079) and overall survival (p = 0.036, log-rank test = 4.414). These findings indicate parafibromin may be related to the pathogenesis of HCC and a potential prognostic marker for HCC patients after hepatectomy.
Impaired Endothelial Function in Convalescent Phase of COVID-19: A 3 Month Follow Up Observational Prospective Study
Authors: Santoro Luca , Falsetti Lorenzo , Zaccone Vincenzo , Nesci Antonio , Tosato Matteo , Giupponi Bianca , Savastano Maria Cristina , Moroncini Gianluca , Gasbarrini Antonio , Landi Francesco , Santoliquido Angelo ,   on behalf of Gemelli against COVID-19 Post-Acute Care Study Group
Published: March 2022

Background: Endothelial dysfunction has a role in acute COVID-19, contributing to systemic inflammatory syndrome, acute respiratory distress syndrome, and vascular events. Evidence regarding COVID-19 middle- and long-term consequences on endothelium are still lacking. Our study aimed to evaluate if COVID-19 severity could significantly affect the endothelial function after three months from the acute phase. Methods: We assessed endothelial function in outpatients with previous COVID-19 three months after negative SARS-CoV-2 molecular test by measuring flow-mediated dilation (FMD) in patients categorized according to a four-variable COVID-19 severity scale (“home care”; “hospital, no oxygen”; “hospital, oxygen”; “hospital requiring high-flow nasal canula, non-invasive ventilation, invasive mechanical ventilation, or extracorporeal membrane oxygenation”). FMD difference among COVID-19 severity categories was assessed with analysis of variance; we further clarified the relationship between FMD and previous COVID-19 severity with multivariate logistic models. Results: Among 658 consecutive COVID-19 subjects, we observed a significant linear trend of FMD reduction with the increase of the COVID-19 category (p < 0.0001). The presence of endothelial dysfunction was more frequent among hospitalized patients (78.3%) with respect to home-care patients (21.7%; p < 0.0001). COVID-19 severity was associated with increased endothelial dysfunction risk (OR: 1.354; 95% CI: 1.06–1.71; p = 0.011) at multivariate binary logistic analysis. FMD showed a significant direct correlation with PaO2 (p = 0.004), P/F ratio (p = 0.004), FEV1 (p = 0.008), and 6MWT (p = 0.0001). Conclusions: Hospitalized COVID-19 subjects showed an impaired endothelial function three months after the acute phase that correlated with pulmonary function impairment. Further studies are needed to evaluate if these subjects are at higher risk of developing pulmonary disease or future cardiovascular events.
Randomized Clinical Trial Evaluating AcceptME—A Digital Gamified Acceptance and Commitment Early Intervention Program for Individuals at High Risk for Eating Disorders
Authors: Karekla Maria , Nikolaou Patrisia , Merwin Rhonda M.
Published: March 2022

Eating disorders (ED) constitute a serious public health issue affecting predominantly women and appearing typically in adolescence or early adulthood. EDs are extremely difficult to treat, as these disorders are ego-syntonic, and many patients do not seek treatment. It is vital to focus on the development of successful early-intervention programs for individuals presenting at risk and are on a trajectory towards developing EDs. This study is a randomized controlled trial evaluating an innovative digital gamified Acceptance and Commitment early-intervention program (AcceptME) for young females showing signs and symptoms of an ED and at high risk for an ED. Participants (n = 92; Mage = 15.30 years, SD = 2.15) received either AcceptME (n = 62) or a waitlist control (n = 30). Analyses indicated that the AcceptME program effectively reduced weight and shape concerns with large effects when compared to waitlist controls. Most participants scored below the at-risk cut-off (WCS score < 52) in the AcceptME at end-of-intervention (57.1%) compared to controls (7.1%), with odds of falling into the at-risk group being 14.5 times higher for participants in the control group. At follow-up, 72% of completers reported scores below the at-risk cut-off in the AcceptME group. The intervention also resulted in a decrease in ED symptomatology and increased body image flexibility. Overall, results suggest that the AcceptME program holds promise for early-intervention of young women at risk for developing an ED.
In BCR-ABL1 Positive B-Cell Acute Lymphoblastic Leukemia, Steroid Therapy Induces Hypofibrinogenemia
Authors: Buzzatti Elisa , Forghieri Fabio , Paterno Giovangiacinto , Marchesi Francesco , Sarlo Chiara , Giglio Fabio , Fracchiolla Nicola , Sciumè Mariarita , Palmieri Raffaele , Esposito Fabiana , Guarnera Luca , Mercante Lisa , Pascale Maria Rosaria , Mallegni Flavia , Savi Arianna , Forte Vittorio , Maurillo Luca , Buccisano Francesco , Venditti Adriano , Del Principe Maria Ilaria
Published: March 2022

Hypofibrinogenemia (HF) in adult acute lymphoblastic leukemia (ALL) of B lineage is uncommon and mostly associated with asparaginase (ASP) delivery. Since we noticed a significant reduction in fibrinogen (FBG) plasma levels even before the first ASP dose, we aim to assess the levels of FBG during induction treatment and explore if the FBG fall correlated with therapies other than asparaginase and/or specific leukemia biological features. We retrospectively analyzed FBG levels in 115 patients with B-ALL. In 74 (64%) out of 115 patients FBG decline occurred during the steroid prephase. In univariate analysis, such a steroid-related HF was significantly associated with BCR-ABL1 rearrangement (p = 0.00158). None of those experiencing HF had significant modifications of liver function tests during induction treatment. Our retrospective study suggests that in B-ALL, steroid therapy can also induce HF and that such an event is preferentially observed in patients carrying BCR-ABL1 rearrangements. The pathogenesis of this phenomenon is still unclear. We attempt to explain it by applying the International Society of Thrombosis and Hemostasis-Disseminated Intravascular Coagulation score (ISTH-DIC score); nonetheless additional studies are needed to clarify further the mechanisms of HF in this subset of patients.
Effects of Foam Rolling vs. Manual Therapy in Patients with Tension-Type Headache: A Randomized Pilot Study
Authors: Espi-Lopez Gemma V. , Ingles Marta , Carrasco-Fernandez Juan J. , Serra-Añó Pilar , Copete-Fajardo Luis , Gonzalez-Gerez Juan Jose , Saavedra-Hernandez Manuel , Marques-Sule Elena
Published: March 2022

Background: This study compares the effect of foam rolling (FR) vs. manual therapy (MT) on pain, pressure pain threshold (PPT), headache disability (HDI) and impact of headache (HIT-6) in patients with tension-type headache (TTH). Methods: A total of 38 participants with TTH were randomly assigned to an FR group (FRG, n = 13), an MT group (MTG, n = 13) or a control group (CG, n = 12). FRG received FR treatment; MTG received MT techniques; CG received a placebo treatment. The treatment lasted one month. Outcome measures were assessed at baseline, post-intervention and follow-up. Results: Both FRG and MTG showed significant improvements in all variables after the treatment, but the intervention effect was maintained only for functional disability (p = 0.002 and p = 0.005, respectively), overall disability (p = 0.007; p = 0.030) and HIT-6 (p = 0.002; p = 0.001) at follow-up. After treatment, FRG and MTG presented a significantly higher PPT in right (p = 0.044; p = 0.009) and left suboccipital (p = 0.004; p = 0.021). MTG showed a significantly lower HIT-6 than CG (p = 0.008). No differences between FRG and MTG were found in any variable. Conclusions: Both FR and MT are effective treatments for the improvement of clinical symptoms in TTH. Further studies are needed to confirm our findings in a larger population.
Recombinase Polymerase Amplification Combined with Real-Time Fluorescent Probe for Mycoplasma pneumoniae Detection
Authors: Jiang Tingting , Wang Yacui , Jiao Weiwei , Song Yiqin , Zhao Qing , Wang Tianyi , Bi Jing , Shen Adong
Published: March 2022

Mycoplasma pneumoniae (M. pneumoniae) is one of the major causes of community-acquired pneumonia, accounting for 20–40% of total cases. Rapid and accurate detection of M. pneumoniae is crucial for the diagnosis and rational selection of antibiotics. In this study, we set up a real-time recombinase polymerase amplification (RPA) assay to detect the conserved gene CARDS of M. pneumoniae. The amplification can be finished in 20 min at a wide temperature range from 37–41 °C. The limit of detection of RPA assay was 10 fg per microliter. Cross-reaction with commonly detected respiratory pathogens was not observed using RPA assay. Among clinical sputum samples, the detection rate of RPA assay and real-time PCR assay was 48.4% (92/190) and 46.3% (88/190), respectively (p = 0.68). Therefore, the RPA assay for M. pneumoniae detection is rapid and easy to use and may serve as a promising test for early diagnosis of M. pneumoniae infection.
Metabolic Syndrome, Gamma-Glutamyl Transferase, and Risk of Sudden Cardiac Death
Authors: Kim Yun Gi , Han Kyungdo , Jeong Joo Hee , Roh Seung-Young , Choi Yun Young , Min Kyongjin , Shim Jaemin , Choi Jong-Il , Kim Young-Hoon
Published: March 2022

Background: Metabolic syndrome is associated with a significantly increased risk of sudden cardiac death (SCD). However, whether temporal changes in the metabolic syndrome status are associated with SCD is unknown. We aimed to determine whether metabolic syndrome and gamma-glutamyl transferase (ɣ-GTP), including their temporal changes, are associated with the risk of SCD. Methods: We performed a nationwide population-based analysis using the Korean National Health Insurance Service. People who underwent a national health check-up in 2009 and 2011 were enrolled. The influence of metabolic syndrome and ɣ-GTP on SCD risk was evaluated. Results: In 2009, 4,056,423 (848,498 with metabolic syndrome) people underwent health screenings, 2,706,788 of whom underwent follow-up health screenings in 2011. Metabolic syndrome was associated with a 50.7% increased SCD risk (adjusted hazard ratio (aHR) = 1.507; p < 0.001). The SCD risk increased linearly as the metabolic syndrome diagnostic criteria increased. The ɣ-GTP significantly impacted the SCD risk; the highest quartile had a 51.9% increased risk versus the lowest quartile (aHR = 1.519; p < 0.001). A temporal change in the metabolic syndrome status and ɣ-GTP between 2009 and 2011 was significantly correlated with the SCD risk. Having metabolic syndrome in 2009 or 2011 indicated a lower SCD risk than having metabolic syndrome in 2009 and 2011 but a higher risk than having no metabolic syndrome. People with a ≥20-unit increase in ɣ-GTP between 2009 and 2011 had an 81.0% increased SCD risk versus those with a change ≤5 units (aHR = 1.810; p < 0.001). Conclusions: Metabolic syndrome and ɣ-GTP significantly correlated with an increased SCD risk. SCD was also influenced by temporal changes in the metabolic syndrome status and ɣ-GTP, suggesting that appropriate medical treatment and lifestyle modifications may reduce future SCD risk.
Synergistic Antibacterial Effect of Zinc Oxide Nanoparticles and Polymorphonuclear Neutrophils
Authors: Ye Kai , Huang Moran , He Xiaojian , An Zhiquan , Qin Hui
Published: March 2022

Zinc oxide nanoparticles (ZnONPs) are inorganic nano-biomaterials with excellent antimicrobial properties. However, their effects on the anti-infection ability of the innate immune system remains poorly understood. The aim of the present study was to explore the potential immunomodulatory effects of ZnONPs on the innate immune system, represented by polymorphonuclear leukocytes (PMNs), and determine whether they can act synergistically to resist pathogen infections. In vitro experiment showed that ZnONPs not only exhibit obvious antibacterial activity at biocompatible concentrations but also enhance the antibacterial property of PMNs. In vivo experiments demonstrated the antibacterial effect of ZnONPs, accompanied by more infiltration of subcutaneous immune cells. Further ex vivo and in vitro experiments revealed that ZnONPs enhanced the migration of PMNs, promoted their bacterial phagocytosis efficiency, proinflammatory cytokine (TNF-α, IL-1β, and IL-6) expression, and reactive oxygen species (ROS) production. In summary, this study revealed potential synergistic effects of ZnONPs on PMNs to resist pathogen infection and the underlying mechanisms. The findings suggest that attempts should be made to fabricate and apply biomaterials in order to maximize their synergy with the innate immune system, thus promoting the host’s resistance to pathogen invasion.
Structural and Functional Analyses of Motile Fauna Associated with Cystoseira brachycarpa along a Gradient of Ocean Acidification in a CO2-Vent System off Panarea (Aeolian Islands, Italy)
Authors: Esposito Valentina , Auriemma Rocco , De Vittor Cinzia , Relitti Federica , Urbini Lidia , Kralj Martina , Gambi Maria Cristina
Published: March 2022

Ocean acidification (OA), one of the main climate-change-related stressors linked to increasing CO2 concentration in the atmosphere, is considered an important threat to marine biodiversity and habitats. Studies on CO2-vents systems, naturally acidified environments that mimic future ocean scenarios, help to explore the sensitivity of species and to understand how benthic communities rearrange their structure and functioning under the pressure of OA. We addressed this problem by studying the benthic invertebrates associated with a habitat-forming brown alga (Cystoseira brachycarpa) in the Bottaro crater vents system off Panarea island (Tyrrhenian Sea, Italy), by sampling along an OA gradient from the proximity of the main venting area (station B3, pH 7.9) to a control zone (B1 station, pH 8.1). Samples were collected in September 2016 and 2018. A total of 184 taxa and 23 different functional traits have been identified, considering feeding habit, motility, size, reproductive and developmental biology, and occurrence of calcareous structures. Invertebrates are distributed according to the distance from the high venting zone and low pH levels and results very consistent between the two investigated years. In the low-pH area (B3), 43% of the species are selected. The functional traits of the fauna mirror this zonation pattern, mainly changing the relative proportion of the number of individuals of the various functional guilds along the OA gradient. Invertebrates inhabiting the low-pH zone are mainly composed of weakly or non-calcified species, with small size, burrower/tubicolous habit, omnivorous or suspension feeders, and with direct development and brooding habit. In the other stations, heavily calcified forms, herbivore and herbivore/detritivore, and with medium (1–5 cm) and large (>5 cm) sizes prevail, showing indirect benthic and planktic development. The taxonomic analysis, coupled with functional aspects, increases our prediction of which traits could be potentially more advantageous for species to adapt to the hypothesized scenarios of OA, and identify present and future winner and/or loser organisms in the future ocean of the Anthropocene.
Quality Analysis of AISI 321 Welds of Bellow Compensators Used in Shipbuilding
Authors: Marić Dejan , Cumin Josip , Šolić Tomislav , Samardžić Ivan
Published: March 2022

The production of compensators (expansion joints), and especially of bellows as their most demanding elements, requires the usage of stainless materials. These stainless materials exhibit certain particularities at welding (and quality control) since they are very thin, i.e., their thickness ranges usually from 0.12 to 3.00 mm. This paper starts with the elaboration of compensators and their application, and further presents characteristics of the material selected for experimental testing. In the second part, the paper continues with a description of the experiment referring to longitudinal welding of samples without filler material. The experiment focused on the determination of accurate characteristics of radiographic recordings and their assessment based on comparison with the tensile testing (mechanical properties), FEM numerical simulation and dimensional control. The paper also presents the analysis of obtained results and conclusions made thereof. The research hypothesis was to determine in what way the welding parameters affect the mechanical properties, the geometric shape of the welds, and the exploitation possibilities of the compensator. According to the performed experimental part and the performed testing of mechanical properties, all welded joints are acceptable for use. Nevertheless, according to the standard that prescribes the acceptability criteria of defects that occur in welded joints, some of tested samples were not suitable for operating conditions. Such kinds of welded sheets must be repaired or eliminated from further processing.
Morphological and Molecular Identification of Plant Pathogenic Fungi Associated with Dirty Panicle Disease in Coconuts (Cocos nucifera) in Thailand
Authors: Sunpapao Anurag , Suwannarach Nakarin , Kumla Jaturong , Dumhai Reajina , Riangwong Kanamon , Sanguansub Sunisa , Wanchana Samart , Arikit Siwaret
Published: March 2022

Dirty panicle disease in coconuts (Cocos nucifera) was first observed in the KU-BEDO Coconut BioBank, Nakhon Pathom province, Thailand. The occurrence of the disease covers more than 30% of the total coconut plantation area. The symptoms include small brown to dark brown spots and discoloration of male flowers. Herein, three fungal strains were isolated from infected samples. Based on the morphological characteristics the fungal isolates, they were classified into two genera, namely, Alternaria (Al01) and Fusarium (FUO01 and FUP01). DNA sequences of internal transcribed spacer (ITS), glyceraldehyde 3-phosphate dehydrogenase (GAPDH), translation elongation factor 1-α (tef1-α), and RNA polymerase II second largest subunit (rpb2) revealed Al01 as Alternaria burnsii, whereas DNA sequences of ITS, rpb2, and tef1-α identified FUO01 and FUP01 as Fusarium clavum and F. tricinctum, respectively. A pathogenicity test by the agar plug method demonstrated that these pathogens cause dirty panicle disease similar to that observed in natural infections. To the best of our knowledge, this is the first report on the novel dirty panicle disease in coconuts in Thailand or elsewhere, demonstrating that it is associated with the plant pathogenic fungi A. burnsii, F. clavum, and F. tricinctum.
Sex Differences in Clinical Characteristics and Outcomes of Patients with SARS-CoV-2-Infection Admitted to Intensive Care Units in Austria
Authors: Kautzky-Willer Alexandra , Kaleta Michaela , Lindner Simon D. , Leutner Michael , Thurner Stefan , Klimek Peter
Published: March 2022

Importance: A male predominance is reported in hospitalised patients with COVID-19 alongside a higher mortality rate in men compared to women. Objective: To assess if the reported sex bias in the COVID-19 pandemic is validated by analysis of a subset of patients with severe disease. Design: A nationwide retrospective cohort study was performed using the Austrian National COVID Database. We performed a sex-specific Lasso regression to select the covariates best explaining the outcomes of mechanical ventilation and death using variables known before ICU admission. We use logistic regression to construct a sex-specific “risk score” for the outcomes using these variables. Setting: We studied the characteristics and outcomes of patients admitted to intensive care units (ICUs) in Austria. Participants: 5118 patients admitted to the ICU in Austria with a COVID-19 diagnosis in 03/2020–03/2021. Exposures: Demographic and clinical characteristics, vital signs and laboratory tests, comorbidities, and management of patients admitted to ICUs were analysed for possible sex differences. Main outcomes and measures: The aim was to define risk scores for mechanical ventilation and mortality for each sex to provide better sex-sensitive management and outcomes in the future. Results: We found balanced accuracies between 55% and 65% to predict the outcomes. Regarding outcome death, we found that the risk score for pre-ICU variables increases with age, renal insufficiency (f: OR 1.7(2), m: 1.9(2)) and decreases with observance as admission cause (f: OR 0.33(5), m: 0.36(5)). Additionally, the risk score for females also includes respiratory insufficiency (OR 2.4(4)) while heart failure for males only (OR 1.5(1)). Conclusions and relevance: Better knowledge of how sex influences COVID-19 outcomes at ICUs will have important implications for the ongoing pandemic’s clinical care and management strategies. Identifying sex-specific features in individuals with COVID-19 and fatal consequences might inform preventive strategies and public health services.