bluuebunny/crossref_metadata_2025 huggingface dataset:
Skills Intelligence in the Steel Sector
Authors: Maldonado-Mariscal Karina , Cuypers Mathias , Götting Adrian , Kohlgrüber Michael
Published: March 2023

The ecological and digital transformations of the steel industry intensify already existing skill shortages and create specific skill demands that are currently not being met. One of the main problems in this sector lies in the lack of sufficient information on which skills companies need and which skills trainings are suitable for today’s challenges. In addition, more information is needed to provide more and better information for policy-making processes for getting the sector’s workforce well-equipped for digitalisation and decarbonisation. This paper uses the framework of skills intelligence in the steel sector, reflecting on theoretical developments and the application of concrete tools in the European projects BEYOND 4.0 and ESSA. The main research questions guiding this work are: To what extent is the concept of skills intelligence useful in the steel sector, and how can it be applied in the steel sector in Europe? This paper provides empirical data based on qualitative and quantitative research carried out in the mentioned projects. The main contribution of this paper is the development of concrete reflections on the concept of skills intelligence based on tools in the steel sector. This work operationalises the skills intelligence approach at sectoral level, namely for the steel industry, and shows how this sector approach can be implemented at the European, national and regional levels. The main findings suggest that skills intelligence in the steel sector is not limited to the preparation and presentation of data but creates a governance structure to mitigate skills imbalances.
A New Construction of Weightwise Perfectly Balanced Functions with High Weightwise Nonlinearity
Authors: Zhao Qinglan , Jia Yu , Zheng Dong , Qin Baodong
Published: March 2023

The FLIP cipher was proposed at Eurocrypt 2016 for the purpose of meliorating the efficiency of fully homomorphic cryptosystems. Weightwise perfectly balanced Boolean functions meet the balancedness requirement of the filter function in FLIP ciphers, and the construction of them has attracted serious attention from researchers. Nevertheless, the literature is still thin. Modifying the supports of functions with a low degree is a general construction technique whose key problem is to find a class of available low-degree functions. We first seek out a class of quadratic functions and then, based on these functions, present the new construction of weightwise perfectly balanced Boolean functions by adopting an iterative approach. It is worth mentioning that the functions we construct have good performance in weightwise nonlinearity. In particular, some p-weight nonlinearities achieve the highest values in the literature for a small number of variables.
A Features-Based Privacy Preserving Assessment Model for Authentication of Internet of Medical Things (IoMT) Devices in Healthcare
Authors: Khan Habib Ullah , Ali Yasir , Khan Faheem
Published: March 2023

Internet of Things (IoT) devices have drawn significant attention over the last few years due to their significant contribution to every domain of life, but the major application of these devices has been witnessed in the healthcare sector. IoT devices have changed the complexion of healthcare set-up, however, the major limitation of such devices is susceptibility to many cyberattacks due to the use of embedded operating systems, the nature of communication, insufficient software updates, and the nature of backend resources. Similarly, they transfer a huge amount of sensitive data via sensors and actuators. Therefore, the security of Internet of Health Things (IoHT) devices remains a prime concern as these devices are prone to various cyberattacks, which can lead to compromising and violating the security of IoT devices. Therefore, IoT devices need to be authenticated before they join the network or communicate within a network, and the applied method of authentication must be robust and reliable. This authentication method has to be evaluated before being implemented for the authentication of IoT devices/equipment in a healthcare environment. In this study, an evaluation framework is introduced to provide a reliable and secure authentication mechanism based on authentication features. The proposed framework evaluates and selects the most appropriate authentication scheme/method based on evaluating authentication features using a hybrid multicriteria decision-making approach. It completes this in two steps: in the first step, the analytic hierarchy process (AHP) method is applied for assigning criteria weights; and in the second step, the technique for order preference by similarity to ideal solution (TOPSIS) approach selects the best authentication solution for IoHT devices based upon identified authentication features. This is the first attempt to present a features-based authentication model for selecting the improved authentication solution employed in IoHT devices.
Sensitivity Analysis of Optimal Commodity Decision Making with Neural Networks: A Case for COVID-19
Authors: Karimi Nader , Salavati Erfan , Assa Hirbod , Adibi Hojatollah
Published: March 2023

The COVID-19 pandemic caused a significant disruption to food demand, leading to changes in household expenditure and consumption patterns. This paper presents a method for analyzing the impact of such demand shocks on a producer’s decision to sell a commodity during economic turmoil. The method uses an artificial neural network (ANN) to approximate the optimal value function for a general stochastic differential equation and calculate the partial derivatives of the value function with respect to various parameters of both the diffusion process and the payoff function. This approach allows for sensitivity analysis of the optimal stopping problem and can be applied to a range of situations beyond just the COVID-19 crisis.
Integration of Fuzzy Ontologies and Neural Networks in the Detection of Time Series Anomalies
Authors: Moshkin Vadim , Kurilo Dmitry , Yarushkina Nadezhda
Published: March 2023

This paper explores an approach to solving the problem of detecting time series anomalies, taking into account the specifics of the subject area. We propose a method based on the integration of a neural network with long short-term memory (LSTM) and Fuzzy OWL (Fuzzy Web Ontology Language) ontology. A LSTM network is used for the mathematical search for anomalies in the first stage. The fuzzy ontology filters the detection results and draws an inference for decision making in the second stage. The ontology contains a formalized representation of objects in the subject area and inference rules that select only those anomaly values that correspond to this subject area. In the article, we propose the architecture of a software system that implements this approach. Computational experiments were carried out on free data of technical characteristics of drilling rigs. The experiments showed high efficiency, but not the maximum efficiency of the proposed approach. In the future, we plan to select a more efficient neural network architecture for mathematical anomaly detection. We also plan to develop an algorithm for automatically filling the rules of inference into the ontology when analyzing text sources.
Structural-Health-Monitoring-Oriented Finite Element Model for a Specially Shaped Steel Arch Bridge and Its Application
Authors: Dai Li , Cui Mi-Da , Cheng Xiao-Xiang
Published: March 2023

To rigorously evaluate the health of a steel bridge subjected to vehicle-induced fatigue, both a detailed numerical model and effective fatigue analysis methods are needed. In this paper, the process for establishing the structural health monitoring (SHM)-oriented finite element (FE) model and assessing the vehicle-induced fatigue damage is presented for a large, specially shaped steel arch bridge. First, the bridge is meticulously modeled using multiple FEs to facilitate the exploration of the local structural behavior. Second, manual tuning and model updating are conducted according to the modal parameters measured at the bridge’s location. Since the numerical model comprises a large number of FEs, two surrogate-model-based methods are employed to update the model. Third, the established models are validated by using them to predict the structure’s mode shapes and the actual structural behavior for the case in which the whole bridge is subjected to static vehicle loads. Fourth, using the numerical model, a new fatigue analysis method based on the high-circle fatigue damage accumulation theory is employed to further analyze the vehicle-induced fatigue damage to the bridge. The results indicate that manual tuning and model updating are indispensable for SHM-oriented FE models with erroneous configurations, and one surrogate-model-based model updating method is effective. In addition, it is shown that the fatigue analysis method based on the high-circle fatigue damage accumulation theory is applicable to real-world engineering cases.
Oleaginous Heterotrophic Dinoflagellates—Crypthecodiniaceae
Authors: Kwok Alvin Chun Man , Law Stanley Ping Chuen , Wong Joseph Tin Yum
Published: March 2023

The heterotrophic Crypthecodinium cohnii is a major model for dinoflagellate cell biology, and a major industrial producer of docosahexaenoic acid, a key nutraceutical and added pharmaceutical compound. Despite these factors, the family Crypthecodiniaceae is not fully described, which is partly attributable to their degenerative thecal plates, as well as the lack of ribotype-referred morphological description in many taxons. We report here significant genetic distances and phylogenetic cladding that support inter-specific variations within the Crypthecodiniaceae. We describe Crypthecodinium croucheri sp. nov. Kwok, Law and Wong, that have different genome sizes, ribotypes, and amplification fragment length polymorphism profiles when compared to the C. cohnii. The interspecific ribotypes were supported by distinctive truncation-insertion at the ITS regions that were conserved at intraspecific level. The long genetic distances between Crypthecodiniaceae and other dinoflagellate orders support the separation of the group, which includes related taxons with high oil content and degenerative thecal plates, to be ratified to the order level. The current study provides the basis for future specific demarcation-differentiation, which is an important facet in food safety, biosecurity, sustainable agriculture feeds, and biotechnology licensing of new oleaginous models.
Modulation of SREBP Expression and Fatty Acid Levels by Bacteria-Induced ER Stress Is Mediated by Hemocyanin in Penaeid Shrimp
Authors: Huang Zishu , Zheng Xiaoyu , Chen Zeyan , Zheng Zhihong , Yao Defu , Yang Shen , Zhang Yueling , Aweya Jude Juventus
Published: March 2023

Many environmental and pathogenic insults induce endoplasmic reticulum (ER) stress in animals, especially in aquatic ecosystems, where these factors are crucial for life. In penaeid shrimp, pathogens and environmental stressors induce hemocyanin expression, but the involvement of hemocyanin in ER stress response is unknown. We demonstrate that in response to pathogenic bacteria (Vibrio parahaemolyticus and Streptococcus iniae), hemocyanin, ER stress proteins (Bip, Xbp1s, and Chop), and sterol regulatory element binding protein (SREBP) are induced to alter fatty acid levels in Penaeus vannamei. Interestingly, hemocyanin interacts with ER stress proteins to modulate SREBP expression, while ER stress inhibition with 4-Phenylbutyric acid or hemocyanin knockdown attenuates the expression of ER stress proteins, SREBP, and fatty acid levels. Contrarily, hemocyanin knockdown followed by tunicamycin treatment (ER stress activator) increased their expression. Thus, hemocyanin mediates ER stress during pathogen challenge, which consequently modulates SREBP to regulate the expression of downstream lipogenic genes and fatty acid levels. Our findings reveal a novel mechanism employed by penaeid shrimp to counteract pathogen-induced ER stress.
Vascular Factors in Patients with Midlife Sensorineural Hearing Loss and the Progression to Mild Cognitive Impairment
Authors: Del Vecchio Valeria , Tricarico Laura , Pisani Anna , Serra Nicola , D’Errico Domenico , De Corso Eugenio , Rea Teresa , Picciotti Pasqualina M. , Laria Carla , Manna Giuseppe , Franzè Annamaria , Malesci Rita , Fetoni Anna Rita
Published: March 2023

Background and Objectives: Midlife hearing loss (HL) has been considered as a major modifiable risk factor for a later-life progression to dementia. Our aim was to detect a link between precocious sensorineural hearing loss (SNHL) and mild cognitive impairment (MCI) and their association to putative risk factors for a common pathology. Materials and methods: In this study, a retrospective case-control study was carried out. A total of 112 patients were enrolled as following: 81 patients with bilateral SNHL and 31 subjects with normal hearing, whose ages ranged from 50 to 65 years. Both groups performed pure tone audiometry, a tinnitus handicap inventory (THI), Mini-Mental State examination (MMSE), and the Montreal Cognitive Assessment (MoCA), Hospital Anxiety and Depression Scale (HADS-A and HADS-D). Results: The mean age was 58 ± 5.2 in SNHL patients and 53.2 ± 4.8 in the control group. The mean pure tone average in the SNHL group was 40.2 ± 18.7 dB HL on the right side and 41.2 ± 17.2 dB HL on the left side, while in the control group it was 12.5 ± 2.8 dB HL on right side and 12.4 ± 3.1 dB HL on left side. About 64% of patients with SNHL exhibited comorbidities, and the most common condition was hypertension. Altered MoCA test scores were significantly related to the pure tone averages in patients with SNHL compared to the control group (p = 0.0004), while the differences in the HADS-A and HADS-D were not significant. Furthermore, a significant correlation was observed in SNHL patients between an altered MoCA test and hypercholesterolemia (p = 0.043). Conclusions: Hearing impairment and screening tests to detect MCI should be considered in the midlife in order to carry out strategies to prevent the progression to dementia. Hypertension and hypercholesterolemia are two risk factors in the development of endothelial dysfunction, oxidative stress, and vascular inflammation, and may represent the common pathology linking the inner ear and brain damage.
Everyday Evaluation of Herb/Dietary Supplement–Drug Interaction: A Pilot Study
Authors: Souza-Peres Joao Victor , Flores Kimberly , Umloff Bethany , Heinan Michelle , Herscu Paul , Babos Mary Beth
Published: March 2023

A lack of reliable information hinders the clinician evaluation of suspected herb–drug interactions. This pilot study was a survey-based study conceived as a descriptive analysis of real-life experiences with herb–drug interaction from the perspective of herbalists, licensed health-care providers, and lay persons. Reported dietary supplement–drug interactions were evaluated against the resources most commonly cited for the evaluation of potential supplement–drug interactions. Disproportionality analyses were performed using tools available to most clinicians using data from the U.S. Federal Adverse Event Reporting System (FAERS) and the US Center for Food Safety and Applied Nutrition (CFSAN) Adverse Event Reporting System (CAERS). Secondary aims of the study included exploration of the reasons for respondent use of dietary supplements and qualitative analysis of respondent’s perceptions of dietary supplement–drug interaction. While agreement among reported supplement–drug interactions with commonly cited resources for supplement–drug interaction evaluation and via disproportionality analyses through FAERS was low, agreement using data from CAERS was high.
Mechanism of Chromium Separation and Concentration from Tannery Wastewater by Membrane Methods
Authors: Religa Paweł , Kaźmierczak Bernadetta
Published: March 2023

The article specifies conditions for a nanofiltration process employing a diafiltration method with a constant volume diafiltration (NF-CVD) for exhausted chromium tannery wastewater treatment and describes a mechanism of the examined process carried out for model wastewater. The authors prove that a decrease in salt concentration in the NF-CVD process is an important factor that enables effective concentration of chromium. Based on the proposed chromium separation and concentration mechanisms, it was found that this effect may be achieved by (1) limiting the formation of an ionic adsorption–polarization layer and (2) reducing the increase in the osmotic pressure caused by a change in the separation properties of the membrane. The article shows that in the analyzed system a higher amount of the solvent introduced at the diafiltration stage and a lower process pressure that ensures a reduction in salt retention translate to a high level of salt removal. In regenerates, after the NF-CVD processes in which at least the same volume of a washing diluent as the volume of the retentate after the pre-concentration step was used, salt concentration below 10 g L−1 and chromium concentration about three times higher than in the case of the feed solution were obtained. Therefore, the proposed solution implements the circular economy strategy in the tannery.
Data Independent Acquisition Reveals In-Depth Serum Proteome Changes in Canine Leishmaniosis
Authors: Martinković Franjo , Popović Marin , Smolec Ozren , Mrljak Vladimir , Eckersall Peter David , Horvatić Anita
Published: March 2023

Comprehensive profiling of serum proteome provides valuable clues of health status and pathophysiological processes, making it the main strategy in biomarker discovery. However, the high dynamic range significantly decreases the number of detectable proteins, obstructing the insights into the underlying biological processes. To circumvent various serum enrichment methods, obtain high-quality proteome wide information using the next-generation proteomic, and study host response in canine leishmaniosis, we applied data-independent acquisition mass spectrometry (DIA-MS) for deep proteomic profiling of clinical samples. The non-depleted serum samples of healthy and naturally Leishmania-infected dogs were analyzed using the label-free 60-min gradient sequential window acquisition of all theoretical mass spectra (SWATH-MS) method. As a result, we identified 554 proteins, 140 of which differed significantly in abundance. Those were included in lipid metabolism, hematological abnormalities, immune response, and oxidative stress, providing valuable information about the complex molecular basis of the clinical and pathological landscape in canine leishmaniosis. Our results show that DIA-MS is a method of choice for understanding complex pathophysiological processes in serum and serum biomarker development.
Theoretical and Experimental Study of Friction Characteristics of Textured Journal Bearing
Authors: Wang Hongtao , Bie Wenbo , Zhang Shaolin , Liu Tengfei
Published: March 2023

The proposed lubrication theory of textured journal bearing is a major innovation in the study of the tribological properties of surface morphology. When it comes to the study of surface topography, it is essential to consider the effect of surface roughness when analyzing the characteristics of journal bearing. In this paper, a Reynolds equation containing longitudinal roughness is established for journal bearing and solved by the finite difference principle to obtain the bearing load and friction characteristics. Subsequently, a combination of laser etching and ultrasonic vibration milling processes was used to prepare 5 µm, 20 µm, and 40 µm bearing friction subsets with square micro-texture surfaces. The analysis of the results shows that the surface roughness distributed in the non-texture region can substantially increase the oil film pressure. When the roughness profile and the surface weave work together, the presence of a surface texture with an optimum depth of 5 µm within a roughness range of less than 1.6 µm can improve the load-bearing characteristics by a maximum of 43%. In the study of the preparation of textured bearing friction substrate, it was found that laser etching can ablate the surface of the friction substrate to a depth greater than 20 µm with the ideal effect, while the surface texturing to a depth of 5 µm is more suitable using an ultrasonic vibration processing process. In the simplified journal bearing operating condition, the frictional wear test shows that if the effect of roughness is considered, the frictional force of the depth of 20 µm and 40 µm is significantly reduced and changes less with increasing load, while the frictional force of the textured frictional pair with a depth of 5 µm is improved but significantly affected by the load carrying capacity. Therefore, when the difference between the roughness profile and the depth of the texture is of a small order of magnitude, it indicates that the effect caused by the roughness factor is not negligible.
Femtosecond Laser Processing Assisted SiC High-Temperature Pressure Sensor Fabrication and Performance Test
Authors: Zhao You , Zhao Yulong , Wang Lukang , Yang Yu , Wang Yabing
Published: March 2023

Due to material plastic deformation and current leakage at high temperatures, SOI (silicon-on-insulator) and SOS (silicon-on-sapphire) pressure sensors have difficulty working over 500 °C. Silicon carbide (SiC) is a promising sensor material to solve this problem because of its stable mechanical and electrical properties at high temperatures. However, SiC is difficult to process which hinders its application as a high-temperature pressure sensor. This study proposes a piezoresistive SiC pressure sensor fabrication method to overcome the difficulties in SiC processing, especially deep etching. The sensor was processed by a combination of ICP (inductive coupled plasma) dry etching, high-temperature rapid annealing and femtosecond laser deep etching. Static and dynamic calibration tests show that the accuracy error of the fabricated sensor can reach 0.33%FS, and the dynamic signal response time is 1.2 μs. High and low temperature test results show that the developed sensor is able to work at temperatures from −50 °C to 600 °C, which demonstrates the feasibility of the proposed sensor fabrication method.
Structural Consequences of Post-Synthetic Modification of Cu2P3I2
Authors: Schwenk Gregory R. , Walters John T. , Ji Hai-Feng
Published: March 2023

In an attempt to widen the family of Phosphorus Metal Halides (MxPyXz) and enable new applications, post-synthetic modifications to the MxPyXz, Cu2P3I2 have been reported. While such a technique suggests access to an entirely new family of MxPyXz-based materials, we report, in this work, that the ion-exchange process seemingly influences important properties such as the crystallographic pattern and vibrational modes.
E-2 Glycoprotein Structural Variations Analysed within the CSFV 2.2. Genogroup in a “Closed Grid” Sampling Study from Meghalaya, India
Authors: Mukherjee Priyanka , Ghatak Sandeep , Puro Kekungo , Das Samir , Milton Arockiasamy Arun Prince , Borah Probodh , Chakroborty Amit , Sen Arnab
Published: March 2023

CSF is enzootic in most of pig-producing states, particularly in the NorthEastern (NE) region of India. In this study, a total of 249 sera and 190 tissue samples were collected from different parts of Meghalaya. Samples were processed by ELISA and RT-PCR for serological and molecular diagnosis. Representative positive samples from the Khasi Hills region were selected for sequencing and “close grid” phylogenetic relationship using partial genomic regions of 5′UTR and E2. High seroprevalence (74.7%) of CSFV was recorded. Detection of the CSFV genome in serologically positive serum samples and tissue samples was 61.29% and 18.94%, respectively. BLAST and phylogenetic analyses indicate the clustering of all the field samples in subgroup 2.2, with high identity with EF014334 from China. Molecular structural modelling of the E2 partial sequence using representative sequences MG563797 from Meghalaya and EF014334 from China indicate potential changes in the protein motif and its conformation, which may explain the emergence of subgroup 2.2 CSFV replacing the predominant subgroup 1.1 viruses in NorthEast India. The epidemiological information presented in this study may be helpful for determination of disease incidence in this region, whereas the virus profile may be useful for framing disease control programs.
Animal Models for Studying Congenital Transmission of Hepatitis E Virus
Authors: Yadav Kush Kumar , Kenney Scott P.
Published: March 2023

One of the most intriguing issues in the hepatitis E virus (HEV) field is the significant increase in mortality rates of the mother and fetus when infection occurs in the second and third trimesters of gestation. A virus that is normally self-limiting and has a mortality rate of less than one percent in otherwise healthy individuals steeply rises by up to 30% in these pregnant populations. Answering this pivotal question has not been a simple task. HEV, in general, has been a difficult pathogen to understand in the laboratory setting. A historical lack of ability to efficiently propagate the virus in tissue culture models has led to many molecular aspects of the viral lifecycle being understudied. Although great strides have been made in recent years to adapt viruses to cell culture, this field remains behind other viruses that are much easier to replicate efficiently in vitro. Some of the greatest discoveries regarding HEV have come from using animal models for which naturally occurring strains of HEV have been identified, including pigs and chickens, but key limitations have made animal models imperfect for studying all aspects of human HEV infections. In addition to the difficulties working with HEV, pregnancy is a very complicated biological process with an elaborate interplay between many different host systems, including hormones, cardiovascular, kidneys, respiratory, gastrointestinal, epithelial, liver, metabolic, immune, and others. Significant differences between the timing and interplay of these systems are notable between species, and making direct comparisons between animals and humans can be difficult at times. No simple answer exists as to how HEV enhances mortality in pregnant populations. One of the best approaches to studying HEV in pregnancy is likely a combinatorial approach that uses the best combination of emerging in vitro and in vivo systems while accounting for the deficiencies that are present in each model. This review describes many of the current HEV animal model systems and the strengths and weaknesses of each as they apply to HEV pregnancy-associated mortality. We consider factors that are critical to analyzing HEV infection within the host and how, despite no perfect animal model for human pregnancy mortality existing, recent developments in HEV models, both in vitro and in vivo, are advancing our overall understanding of HEV in the pregnant host.
Field Use of Protective Bacteriophages against Pectinolytic Bacteria of Potato
Authors: Petrzik Karel , Vacek Josef , Kmoch Martin , Binderová Denisa , Brázdová Sára , Lenz Ondřej , Ševčík Rudolf
Published: March 2023

The pectinolytic Dickeya solani bacterium is an important pathogen found in potatoes. We conducted laboratory and field experiments mimicking severe and mild Dickeya spp. infection and investigated the application of a mixture of two lytic bacteriophages before and after bacterial infection to protect the plants. Application of the phage solution to tuber disks and wounded tubers did not completely eliminate the infection but reduced the development of soft rot symptoms by 59.5–91.4%, depending on the phage concentration. In the field trial, plants treated with bacteriophages after severe Dickeya infection had 5–33% greater leaf cover and 4–16% greater tuber yield compared to untreated plants. When simulating a mild infection, leaf cover was 11–42% greater, and tuber yield was 25–31% greater compared to untreated plants. We conclude that the phage mixture has the potential to protect potatoes ecologically from D. solani.
CRISPR/Cas9-Mediated Multi-Locus Promoter Engineering in ery Cluster to Improve Erythromycin Production in Saccharopolyspora erythraea
Authors: Zhang Xuemei , Wang Yan , Zhang Yue , Wang Meng
Published: March 2023

Erythromycins are a group of macrolide antibiotics produced by Saccharopolyspora erythraea. Erythromycin biosynthesis, which is a long pathway composed of a series of biochemical reactions, is precisely controlled by the type I polyketide synthases and accessary tailoring enzymes encoded by ery cluster. In the previous work, we have characterized that six genes representing extremely low transcription levels, SACE_0716-SACE_0720 and SACE_0731, played important roles in limiting erythromycin biosynthesis in the wild-type strain S. erythraea NRRL 23338. In this study, to relieve the potential bottlenecks of erythromycin biosynthesis, we fine-tuned the expression of each key limiting ery gene by CRISPR/Cas9-mediated multi-locus promoter engineering. The native promoters were replaced with different heterologous ones of various strengths, generating ten engineered strains, whose erythromycin productions were 2.8- to 6.0-fold improved compared with that of the wild-type strain. Additionally, the optimal expression pattern of multiple rate-limiting genes and preferred engineering strategies of each locus for maximizing erythromycin yield were also summarized. Collectively, our work lays a foundation for the overall engineering of ery cluster to further improve erythromycin production. The experience of balancing multiple rate-limiting factors within a cluster is also promising to be applied in other actinomycetes to efficiently produce value-added natural products.
Development of an Extraction Method to Detect Hepatitis A Virus, Hepatitis E Virus, and Noroviruses in Fish Products
Authors: Hennechart-Collette Catherine , Dehan Océane , Fraisse Audrey , Martin-Latil Sandra , Perelle Sylvie
Published: March 2023

Viruses are a leading cause of foodborne disease worldwide. Hepatitis viruses (hepatitis A (HAV) and hepatitis E (HEV)) and human norovirus are recognized as the main viruses of public health concern in food hygiene. ISO 15216 approved procedures are not validated for detection of HAV and human norovirus in foodstuffs, such as fishes, leading to an inability to ensure the safety of these products. This study aimed to provide a rapid and sensitive method for detecting these targets in fish products. An existing method that includes proteinase K treatment was selected for further validation using artificially contaminated fish products, according to the recent international standard ISO 16140-4. Recovery efficiencies in pure RNA extracts of viruses ranged from 0.2% to 66.2% for HAV, 4.0% to 100.0% for HEV, 2.2% to 100.0% for norovirus GI, and 0.2% to 12.5% for norovirus GII. LOD50 values were between 144 and 8.4 × 104 genome copies/g for HAV and HEV, and 104 and 2.0 × 103 copies/g for norovirus GI and norovirus GII, respectively. LOD95 values were between 3.2 × 103 and 3.6 × 105 genome copies/g for HAV and HEV, and between 8.8 × 103 and 4.4 × 104 genome copies/g for norovirus GI and norovirus GII, respectively. The method developed here was successfully validated in various fish products and can be applied for routine diagnostic needs.
The Gut Microbiome of an Indigenous Agropastoralist Population in a Remote Area of Colombia with High Rates of Gastrointestinal Infections and Dysbiosis
Authors: Kann Simone , Eberhardt Kirsten , Hinz Rebecca , Schwarz Norbert Georg , Dib Juan Carlos , Aristizabal Andres , Mendoza Gustavo Andrés Concha , Hagen Ralf Matthias , Frickmann Hagen , Barrantes Israel , Kreikemeyer Bernd
Published: March 2023

An Indigenous agropastoralist population called the Wiwa from the Sierra Nevada de Santa Marta, in North-East Colombia, shows high rates of gastrointestinal infections. Chronic gut inflammatory processes and dysbiosis could be a reason, suggesting an influence or predisposing potential of the gut microbiome composition. The latter was analyzed by 16S rRNA gene amplicon next generation sequencing from stool samples. Results of the Wiwa population microbiomes were associated with available epidemiological and morphometric data and compared to control samples from a local urban population. Indeed, locational-, age-, and gender-specific differences in the Firmicutes/Bacteriodetes ratio, core microbiome, and overall genera-level microbiome composition were shown. Alpha- and ß-diversity separated the urban site from the Indigenous locations. Urban microbiomes were dominated by Bacteriodetes, whereas Indigenous samples revealed a four times higher abundance of Proteobacteria. Even differences among the two Indigenous villages were noted. PICRUSt analysis identified several enriched location-specific bacterial pathways. Moreover, on a general comparative scale and with a high predictive accuracy, we found Sutterella associated with the abundance of enterohemorrhagic Escherichia coli (EHEC), Faecalibacteria associated with enteropathogenic Escherichia coli (EPEC) and helminth species Hymenolepsis nana and Enterobius vermicularis. Parabacteroides, Prevotella, and Butyrivibrio are enriched in cases of salmonellosis, EPEC, and helminth infections. Presence of Dialister was associated with gastrointestinal symptoms, whereas Clostridia were exclusively found in children under the age of 5 years. Odoribacter and Parabacteroides were exclusively identified in the microbiomes of the urban population of Valledupar. In summary, dysbiotic alterations in the gut microbiome in the Indigenous population with frequent episodes of self-reported gastrointestinal infections were confirmed with epidemiological and pathogen-specific associations. Our data provide strong hints of microbiome alterations associated with the clinical conditions of the Indigenous population.
High-Throughput Preparation and High-Throughput Detection of Polymer-Dispersed Liquid Crystals Based on Ink-Jet Printing and Grayscale Value Analysis
Authors: Xiong Rui-Juan , Ren Yun-Xiao , Cui Yong-Feng , Cai Shu-Feng , He Wan-Li , Yuan Xiao-Tao
Published: March 2023

In this paper, based on high-throughput technology, polymer dispersed liquid crystals (PDLC) composed of pentaerythritol tetra (2-mercaptoacetic acid) (PETMP), trimethylolpropane triacrylate (TMPTA), and polyethylene glycol diacrylate (PEGD 600) were investigated in detail. A total of 125 PDLC samples with different ratios were quickly prepared using ink-jet printing. Based on the method of machine vision to identify the grayscale level of samples, as far as we know, it is the first time to realize high-throughput detection of the electro-optical performance of PDLC samples, which can quickly screen out the lowest saturation voltage of batch samples. Additionally, we compared the electro-optical test results of manual and high-throughput preparation PDLC samples and discovered that they had very similar electro-optical characteristics and morphologies. This demonstrated the viability of PDLC sample high-throughput preparation and detection, as well as promising application prospects, and significantly increased the efficiency of PDLC sample preparation and detection. The results of this study will contribute to the research and application of PDLC composites in the future.
New Anti-Glycative Lignans from the Defatted Seeds of Sesamum indicum
Authors: Jeong Gyeong Han , Kim Tae Hoon
Published: March 2023

Seven known analogs, along with two previously undescribed lignan derivatives sesamlignans A (1) and B (2), were isolated from a water-soluble extract of the defatted sesame seeds (Sesamum indicum L.) by applying the chromatographic separation method. Structures of compounds 1 and 2 were elucidated based on extensive interpretation of 1D, 2D NMR, and HRFABMS spectroscopic data. The absolute configurations were established by analyzing the optical rotation and circular dichroism (CD) spectrum. Inhibitory effects against the formation of advanced glycation end products (AGEs) and peroxynitrite (ONOO−) scavenging assays were performed to evaluate the anti-glycation effects of all isolated compounds. Among the isolated compounds, (1) and (2) showed potent inhibition towards AGEs formation, with IC50 values of 7.5 ± 0.3 and 9.8 ± 0.5 μM, respectively. Furthermore, the new aryltetralin-type lignan 1 exhibited the most potent activity when tested in the in vitro ONOO− scavenging assay.
Linum corymbulosum Protects Rats against CCl4-Induced Hepatic Injuries through Modulation of an Unfolded Protein Response Pathway and Pro-Inflammatory Intermediates
Authors: Batool Riffat , Khan Muhammad Rashid , Ijaz Muhammad Umar , Naz Irum , Batool Afsheen , Ali Saima , Zahra Zartash , Gul Safia , Uddin Mohammad N. , Kazi Mohsin , Khan Raees
Published: March 2023

Liver fibrosis is a major pathological feature of chronic liver disease and effective therapies are limited at present. The present study focuses on the hepatoprotective potential of L. corymbulosum against carbon tetrachloride (CCl4)-induced liver damage in rats. Analysis of Linum corymbulosum methanol extract (LCM) using high-performance liquid chromatography (HPLC) revealed the presence of rutin, apigenin, catechin, caffeic acid and myricetin. CCl4 administration lowered (p < 0.01) the activities of antioxidant enzymes and reduced glutathione (GSH) content as well as soluble proteins, whereas the concentration of H2O2, nitrite and thiobarbituric acid reactive substances was higher in hepatic samples. In serum, the level of hepatic markers and total bilirubin was elevated followed by CCl4 administration. The expression of glucose-regulated protein (GRP78), x-box binding protein-1 total (XBP-1 t), x-box binding protein-1 spliced (XBP-1 s), x-box binding protein-1 unspliced (XBP-1 u) and glutamate–cysteine ligase catalytic subunit (GCLC) was enhanced in CCl4-administered rats. Similarly, the expression of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and monocyte chemo attractant protein-1 (MCP-1) was strongly increased with CCl4 administration to rats. Co-administration of LCM along with CCl4 to rats lowered (p < 0.05) the expression of the above genes. Histopathology of the liver showed hepatocyte injury, leukocyte infiltration and damaged central lobules in CCl4-treated rats. However, LCM administration to CCl4-intoxicated rats restored the altered parameters towards the levels of control rats. These outcomes indicate the existence of antioxidant and anti-inflammatory constituents in the methanol extract of L. corymbulosum.
Spatiotemporal Imaging of Zinc Ions in Zebrafish Live Brain Tissue Enabled by Fluorescent Bionanoprobes
Authors: Jarosova Romana , Woolfolk Sarah K. , Martinez-Rivera Noraida , Jaeschke Mathew W. , Rosa-Molinar Eduardo , Tamerler Candan , Johnson Michael A.
Published: March 2023

The zebrafish is a powerful model organism to study the mechanisms governing transition metal ions within whole brain tissue. Zinc is one of the most abundant metal ions in the brain, playing a critical pathophysiological role in neurodegenerative diseases. The homeostasis of free, ionic zinc (Zn2+) is a key intersection point in many of these diseases, including Alzheimer’s disease and Parkinson’s disease. A Zn2+ imbalance can eventuate several disturbances that may lead to the development of neurodegenerative changes. Therefore, compact, reliable approaches that allow the optical detection of Zn2+ across the whole brain would contribute to our current understanding of the mechanisms that underlie neurological disease pathology. We developed an engineered fluorescence protein-based nanoprobe that can spatially and temporally resolve Zn2+ in living zebrafish brain tissue. The self-assembled engineered fluorescence protein on gold nanoparticles was shown to be confined to defined locations within the brain tissue, enabling site specific studies, compared to fluorescent protein-based molecular tools, which diffuse throughout the brain tissue. Two-photon excitation microscopy confirmed the physical and photometrical stability of these nanoprobes in living zebrafish (Danio rerio) brain tissue, while the addition of Zn2+ quenched the nanoprobe fluorescence. Combining orthogonal sensing methods with our engineered nanoprobes will enable the study of imbalances in homeostatic Zn2+ regulation. The proposed bionanoprobe system offers a versatile platform to couple metal ion specific linkers and contribute to the understanding of neurological diseases.
Spectral and Redox Properties of a Recombinant Mouse Cytochrome b561 Protein Suggest Transmembrane Electron Transfer Function
Authors: Bérczi Alajos , Márton Zsuzsanna , Laskay Krisztina , Tóth András , Rákhely Gábor , Duzs Ágnes , Sebők-Nagy Krisztina , Páli Tibor , Zimányi László
Published: March 2023

Cytochrome b561 proteins (CYB561s) are integral membrane proteins with six trans-membrane domains, two heme-b redox centers, one on each side of the host membrane. The major characteristics of these proteins are their ascorbate reducibility and trans-membrane electron transferring capability. More than one CYB561 can be found in a wide range of animal and plant phyla and they are localized in membranes different from the membranes participating in bioenergization. Two homologous proteins, both in humans and rodents, are thought to participate—via yet unidentified way—in cancer pathology. The recombinant forms of the human tumor suppressor 101F6 protein (Hs_CYB561D2) and its mouse ortholog (Mm_CYB561D2) have already been studied in some detail. However, nothing has yet been published about the physical-chemical properties of their homologues (Hs_CYB561D1 in humans and Mm_CYB561D1 in mice). In this paper we present optical, redox and structural properties of the recombinant Mm_CYB561D1, obtained based on various spectroscopic methods and homology modeling. The results are discussed in comparison to similar properties of the other members of the CYB561 protein family.
Study on the Occurrence Difference of Functional Groups in Coals with Different Metamorphic Degrees
Authors: Jia Jinzhang , Xing Yinghuan , Li Bin , Zhao Dan , Wu Yumo , Chen Yinuo , Wang Dongming
Published: March 2023

In order to quantitatively study the difference in occurrence content of functional groups in coals with different metamorphic degrees, the samples of long flame coal, coking coal, and anthracite of three different coal ranks were characterized by FTIR and the relative content of various functional groups in different coal ranks was obtained. The semi-quantitative structural parameters were calculated, and the evolution law of the chemical structure of the coal body was given. The results show that with the increase in the metamorphic degree, the substitution degree of hydrogen atoms on the benzene ring in the aromatic group increases with the increase in the vitrinite reflectance. With the increase in coal rank, the content of phenolic hydroxyl, carboxyl, carbonyl, and other active oxygen-containing groups gradually decreased, and the content of ether bonds gradually increased. Methyl content increased rapidly first and then increased slowly, methylene content increased slowly first and then decreased rapidly, and methylene content decreased first and then increased. With the increase in vitrinite reflectance, the OH-π hydrogen bond gradually increases, the content of hydroxyl self-association hydrogen bond first increases and then decreases, the oxygen-hydrogen bond of hydroxyl ether gradually increases, and the ring hydrogen bond first significantly decreases and then slowly increases. The content of the OH-N hydrogen bond is in direct proportion to the content of nitrogen in coal molecules. It can be seen from the semi-quantitative structural parameters that with the increase in coal rank, the aromatic carbon ratio fa, aromatic degree AR and condensation degree DOC increase gradually. With the increase in coal rank, A(CH2)/A(CH3) first decreases and then increases, hydrocarbon generation potential ‘A’ first increases and then decreases, maturity ‘C’ first decreases rapidly and then decreases slowly, and factor D gradually decreases. This paper is valuable for analyzing the occurrence form of functional groups in different coal ranks and clarifying the evolution process of structure in China.
Evidence Supporting the Involvement of the Minority Compounds of Extra Virgin Olive Oil, through Gut Microbiota Modulation, in Some of the Dietary Benefits Related to Metabolic Syndrome in Comparison to Butter
Authors: Olid María Collado , Hidalgo Marina , Prieto Isabel , Cobo Antonio , Martínez-Rodríguez Ana M. , Segarra Ana Belén , Ramírez-Sánchez Manuel , Gálvez Antonio , Martínez-Cañamero Magdalena
Published: March 2023

Extra virgin olive oil (EVOO) has proven to yield a better health outcome than other saturated fats widely used in the Western diet, including a distinct dysbiosis-preventive modulation of gut microbiota. Besides its high content in unsaturated fatty acids, EVOO also has an unsaponifiable polyphenol-enriched fraction that is lost when undergoing a depurative process that gives place to refined olive oil (ROO). Comparing the effects of both oils on the intestinal microbiota of mice can help us determine which benefits of EVOO are due to the unsaturated fatty acids, which remain the same in both, and which benefits are a consequence of its minority compounds, mainly polyphenols. In this work, we study these variations after only six weeks of diet, when physiological changes are not appreciated yet but intestinal microbial alterations can already be detected. Some of these bacterial deviations correlate in multiple regression models with ulterior physiological values, at twelve weeks of diet, including systolic blood pressure. Comparison between the EVOO and ROO diets reveals that some of these correlations can be explained by the type of fat that is present in the diet, while in other cases, such as the genus Desulfovibrio, can be better understood if the antimicrobial role of the virgin olive oil polyphenols is considered.
Optimized Extraction, Identification and Anti-Biofilm Action of Wu Wei Zi (Fructus Schisandrae Chinensis) Extracts against Vibrio parahaemolyticus
Authors: Zhang Zongyi , Zhao Yanan , Cai Jing , Wang Tong , Song Yujie , Lu Jingyi , Du Hairuo , Wang Wenfang , Zhao Yan , Guo Lei
Published: March 2023

The pathogenicity of foodborne Vibrio parahaemolyticus is a major concern for global public health. This study aimed to optimize the liquid–solid extraction of Wu Wei Zi extracts (WWZE) against Vibrio parahaemolyticus, identify its main components, and investigate the anti-biofilm action. The extraction conditions optimized by the single-factor test and response surface methodology were ethanol concentration of 69%, temperature at 91 °C, time of 143 min, and liquid–solid ratio of 20:1 mL/g. After high performance liquid chromatography (HPLC) analysis, it was found that the main active ingredients of WWZE were schisandrol A, schisandrol B, schisantherin A, schisanhenol, and schisandrin A–C. The minimum inhibitory concentration (MIC) of WWZE, schisantherin A, and schisandrol B measured by broth microdilution assay was 1.25, 0.625, and 1.25 mg/mL, respectively, while the MIC of the other five compounds was higher than 2.5 mg/mL, indicating that schisantherin A and schizandrol B were the main antibacterial components of WWZE. Crystal violet, Coomassie brilliant blue, Congo red plate, spectrophotometry, and Cell Counting Kit-8 (CCK-8) assays were used to evaluate the effect of WWZE on the biofilm of V. parahaemolyticus. The results showed that WWZE could exert its dose-dependent potential to effectively inhibit the formation of V. parahaemolyticus biofilm and clear mature biofilm by significantly destroying the cell membrane integrity of V. parahaemolyticus, inhibiting the synthesis of intercellular polysaccharide adhesin (PIA), extracellular DNA secretion, and reducing the metabolic activity of biofilm. This study reported for the first time the favorable anti-biofilm effect of WWZE against V. parahaemolyticus, which provides a basis for deepening the application of WWZE in the preservation of aquatic products.