bluuebunny/crossref_metadata_2025 huggingface dataset:
Electrical Characterization of Carbon Nanotube Reinforced Silver and Copper Composites for Switching Contacts
Authors: Alderete Bruno , Mücklich Frank , Suarez Sebastian
Published: July 2023

Carbon nanotube (CNT)-reinforced silver and copper metal matrix composites—at three different reinforcement phase concentrations (0.5 wt.%, 0.75 wt.%, and 1 wt.%)—were produced via powder metallurgy and sintered via hot uniaxial pressing. Optical and electron microscopy techniques were used to characterize the powder mixtures and sintered composites. The latter were also electrically characterized via load-dependent electrical contact resistance (ECR) and surface fatigue tests. Particle size and morphology play a crucial role in CNT deposition onto the metallic powder. CNT were deposited exceptionally well onto the dendritic copper powder regardless of its larger size (compared with the silver flakes) due to the higher surface area caused by the grooves and edges of the dendritic structures. The addition of CNT to the metallic matrices improved their electrical performance, in general outperforming the reference material. Higher CNT concentrations produced consistently low ECR values. In addition, high CNT concentrations (i.e., 1 wt.%) show exceptional contact repeatability due to the elastic restitutive properties of the CNT. The reproducibility of the contact surface was further evaluated by the fatigue tests, where the composites also showed lower ECR than the reference material, rapidly reaching steady-state ECR within the 20 fatigue cycles analyzed.
Meningioma-Related Epilepsy: A Happy Ending?
Authors: Pauletto Giada , Nilo Annacarmen , Pez Sara , Zonta Maria Elisa , Bagatto Daniele , Isola Miriam , Verriello Lorenzo , Valente Mariarosaria , Skrap Miran , Ius Tamara
Published: July 2023

(1) Background: More than one-third of patients with meningiomas experience at least one seizure during the course of their disease, and in the 20–50% of cases, seizure represents the onset symptom. After surgery, up to 30% of patients continue to have seizures, while others may experience them later; (2) Methods: The study analyzed retrospectively the risk factors for pre-operative seizures in a large cohort of 358 patients who underwent surgery for newly diagnosed brain meningioma; (3) Results: We identified age, peritumor edema, and location as risk factors for seizure at the onset. Patients with seizures differed from patients without seizures for the following characteristics: younger average age, lower pre-operative Karnofsky Performance Status (KPS), location on the convexity, lower Simpson Grade, lower incidence of pre-operative neurological deficits, and higher incidence of pre-operative peritumor edema. After 24 months, 88.2% of patients were classified as Engel class Ia, and no correlation with disease progression was observed; (4) Conclusions: Meningioma-related epilepsy has generally a positive outcome following surgery and it seems not to be linked to disease progression, even if further studies are needed.
The Role of Coronary CT Angiography in the Evaluation of Dual Left Anterior Descending Artery Prevalence and Subtypes: A Retrospective Multicenter Study
Authors: Maggialetti Nicola , Greco Sara , Lorusso Giovanni , Mileti Cristiana , Sfregola Gabriella , Brunese Maria Chiara , Zappia Marcello , Belfiore Maria Paola , Sullo Pasquale , Reginelli Alfonso , Lucarelli Nicola Maria , Scardapane Arnaldo
Published: July 2023

Background: The aim of this multicenter study was to evaluate the prevalence and features of dual left anterior descending artery (LAD) subtypes using coronary CT angiography (CCTA). Methods: A retrospective multicenter analysis of 2083 CCTA from December 2020 to November 2022 was conducted to search for the presence and morphological features of dual LAD. The two classifications used were the updated classification of Spindola-Franco and the Jariwala classification. Statistical tests were conducted to evaluate the prevalence of dual LADs among sexes and its association with angina in patients without significant coronary stenoses and/or associated cardiac anomalies. Results: Dual LAD was observed in 124 (5.96%) patients analyzed. According to the Spindola-Franco revisited classification, type I dual LAD was the most common (71/124, 57.26%). According to the Jariwala classification, all cases were group I. In the general population, there was a higher prevalence of dual LAD among females (7.3% females vs. 5.1% males; p value: 0.04). No statistically significant difference was found in the prevalence of angina in the dual LAD population compared to the no dual LAD population (2.1% vs. 1.5%; p value: 0.10). Conclusions: The acknowledgment and reporting of LAD duplication is helpful for an optimal management of coronary patients with this condition. Dual LAD was more frequent in the female population, mainly not related with angina. Myocardial bridge was more frequent in the dual LAD population than in the no dual LAD population.
Asymmetric Price Transmission between Crude Oil and the US Gasoline Market
Authors: Kamyabi Najmeh , Chidmi Benaissa
Published: July 2023

Gasoline and crude oil price movements have been the focus of many studies in the last decade. We use the asymmetric error correction model (ERM) to examine the hypothesis of asymmetric pricing for both regular and premium gasoline markets at the US national level and in the four states with the highest gasoline consumption. Using weekly crude oil and retail gasoline prices from June 2000 to February 2023, the results show an asymmetric response in the gasoline market for all four states and at the national level. However, the adjustment speed tends to differ for the types of gasoline and across states. The implications of these results for policy and welfare are discussed in this study.
STARC: Decentralized Coordination Primitive on Low-Power IoT Devices for Autonomous Intersection Management
Authors: Rathje Patrick , Poirot Valentin , Landsiedel Olaf
Published: July 2023

Wireless communication is an essential element within Intelligent Transportation Systems and motivates new approaches to intersection management, allowing safer and more efficient road usage. With lives at stake, wireless protocols should be readily available and guarantee safe coordination for all involved traffic participants, even in the presence of radio failures. This work introduces STARC, a coordination primitive for safe, decentralized resource coordination. Using STARC, traffic participants can safely coordinate at intersections despite unreliable radio environments and without a central entity or infrastructure. Unlike other methods that require costly and energy-consuming platforms, STARC utilizes affordable and efficient Internet of Things devices that connect cars, bicycles, electric scooters, pedestrians, and cyclists. For communication, STARC utilizes low-power IEEE 802.15.4 radios and Synchronous Transmissions for multi-hop communication. In addition, the protocol provides distributed transaction, election, and handover mechanisms for decentralized, thus cost-efficient, deployments. While STARC’s coordination remains resource-agnostic, this work presents and evaluates STARC in a roadside scenario. Our simulations have shown that using STARC at intersections leads to safer and more efficient vehicle coordination. We found that average waiting times can be reduced by up to 50% compared to using a fixed traffic light schedule in situations with fewer than 1000 vehicles per hour. Additionally, we design platooning on top of STARC, improving scalability and outperforming static traffic lights even at traffic loads exceeding 1000 vehicles per hour.
How Does the Smart City Policy Influence Digital Infrastructure? Spatial Evidence from China
Authors: Song Meijing , Xiao Yuan , Zhou Yige
Published: July 2023

With the rapid development of the Internet and digital technology, digital infrastructure has become an important part of urban infrastructure. Many cities are enacting smart policies to promote the development of digital technology infrastructure. However, what are their mechanisms? There is currently a shortage of literature on the subject. This paper tried to solve this problem and used China as an example. Using panel data from cities in China, this paper used the spatial multiple-period difference-in-difference (SDID) method to investigate the impact of smart city policy (SCP) on digital infrastructure. First, we found that SCP significantly promotes the construction of digital infrastructure, with strong positive spatial spillover effects. This result remained valid after a series of rigorous robustness tests. Second, we discovered that the indirect effects of policy implementation outweigh the direct effects. Furthermore, smart city development enhances local government investment in digital infrastructure, attracts more high-tech enterprises, and consequently drives improvements in urban digital infrastructure levels. Lastly, we observed that the effectiveness of smart city policies is stronger in cities with good fiscal conditions, strong economic development, and a thriving digital economy. This research will not only enrich research on smart cities but also provide policy recommendations for strengthening digital infrastructure.
Changes in Salinity, Mangrove Community Ecology, and Organic Blue Carbon Stock in Response to Cyclones at Indian Sundarbans
Authors: Chowdhury Abhiroop , Naz Aliya , Sharma Seema B. , Dasgupta Rajarshi
Published: July 2023

Climate change-induced frequent cyclones are pumping saline seawater into the Sundarbans. Fani, Amphan, Bulbul, and Yaas were the major cyclones that hit the region during 2019–2021. This study represents the changes in the soil parameters, mangrove biodiversity and zonation due to the cyclone surges in the Indian Sundarbans between 2017 and 2021. Increasing tidal water salinity (parts per thousand) trends in both pre-monsoon (21 to 33) and post-monsoon (14 to 19) seasons have been observed between 2017 and 2021. A 46% reduction in the soil organic blue carbon pool is observed due to a 31% increase in soil salinity. Soil organic blue carbon has been calculated by both wet digestion and the elemental analyzer method, which are linearly correlated with each other. A reduction in the available nitrogen (30%) and available phosphorous (33%) in the mangrove soil has also been observed. Salinity-sensitive mangroves, such as Xylocarpus granatum, Xylocarpus moluccensis, Rhizophora mucronata, Bruguiera gymnorrhiza, and Bruguiera cylindrica, have seen local extinction in the sampled population. An increasing trend in relative density of salinity resilient, Avicennia marina, Suaeda maritima, Aegiceras corniculatum and a decreasing trend of true mangrove (Ceriops decandra) has been observed, in response to salinity rise in surface water as well as soil. As is evident from Hierarchical Cluster Analysis (HCA) and the Abundance/Frequency ratio (A/F), the mangrove zonation observed in response to tidal gradient has also changed, becoming more homogeneous with a dominance of A. marina. These findings indicate that cyclone, climate change-induced sea level rise can adversely impact Sustainable Development Goal 13 (climate action), by decreasing organic soil blue carbon sink and Sustainable Development Goal 14 (life below water), by local extinction of salinity sensitive mangroves.
Dementia Is Induced via the AGEs/Iba1/iNOS Pathway in Aged KK-Ay/Tajcl Mice
Authors: Hiramoto Keiichi , Imai Masashi , Tanaka Shota , Ooi Kazuya
Published: July 2023

The onset and exacerbation of dementia have been observed in elderly patients with type 2 diabetes. However, the underlying mechanism remains unclear. In this study, we investigated the effects of aging on the cognitive function in a mouse model of type 2 diabetes. Pathogen-free KK-Ay/TaJcl mice were used in this study. The cognitive abilities and memory declined in the mice and worsened in the 50-week-olds. The levels of advanced glycation end products (AGEs), receptor for AGE (RAGE), and Iba1 in the hippocampus were increased in the mice compared to those in the control mice. Hippocampal levels of CC-chemokine receptor 7 and inducible nitric oxide synthase, which are from M1-type macrophages that shift from microglia, were higher in KK-Ay/TaJcl mice than in control mice. Tumor necrosis factor (TNF)-α and nitric oxide (NO) levels secreted by M1-type macrophages were similarly elevated in the mice and were even higher at the age of 50 weeks. NO levels were markedly elevated in the 50-week-old mice. In contrast, differentiation of CD163 and arginase-1 did not change in both mouse types. Memory and learning declined with age in diabetic mice, and the AGEs/RAGE/M1-type macrophage/NO and TNF-α pathways played an important role in exacerbating memory and learning in those mice.
Sleep Architecture and Daytime Sleepiness in Patients with Erectile Dysfunction
Authors: Martynowicz Helena , Poreba Rafal , Wieczorek Tomasz , Domagala Zygmunt , Skomro Robert , Wojakowska Anna , Winiewska Sylwia , Macek Piotr , Mazur Grzegorz , Gac Paweł
Published: July 2023

Obstructive sleep apnea is considered a risk factor for erectile dysfunction. The aim of this study was to determine sleep architecture and assess daytime sleepiness in patients with erectile dysfunction. The study group included 280 patients. The 107 enrolled patients had reported erectile dysfunction. The control group consisted of 173 patients who had no history of erectile dysfunction. The Epworth sleepiness scale (ESS) was used to measure the subjects’ level of daytime sleepiness. All patients underwent a standardized overnight, single-night polysomnography in sleep laboratory. In the erectile dysfunction group, we observed increased ESS total score and N1 sleep phase duration. Mean and minimal oxygen saturation and mean oxygen desaturation were decreased in comparison to the control group. In summary, subjects with erectile dysfunction have altered sleep architecture, oxygen saturation parameters and increased daytime sleepiness.
Novel LDLR Variant in Familial Hypercholesterolemia: NGS-Based Identification, In Silico Characterization, and Pharmacogenetic Insights
Authors: Athar Mohammad , Toonsi Mawaddah , Abduljaleel Zainularifeen , Bouazzaoui Abdellatif , Bogari Neda M. , Dannoun Anas , Al-Allaf Faisal A.
Published: July 2023

Background: Familial Hypercholesterolemia (FH) is a hereditary condition that causes a rise in blood cholesterol throughout a person’s life. FH can result in myocardial infarction and even sudden death if not treated. FH is thought to be caused mainly by variants in the gene for the low-density lipoprotein receptor (LDLR). This study aimed to investigate the genetic variants in FH patients, verify their pathogenicity, and comprehend the relationships between genotype and phenotype. Also, review studies assessed the relationship between the LDLR null variants and the reaction to lipid-lowering therapy. Methods: The study utilised high-throughput next-generation sequencing for genetic screening of FH-associated genes and capillary sequencing for cascade screening. Furthermore, bioinformatic analysis was employed to describe the pathogenic effects of the revealed novel variant on the structural features of the corresponding RNA molecule. Results: We studied the clinical signs of hypercholesterolemia in a Saudi family with three generations of FH. We discovered a novel frameshift variant (c.666_670dup, p.(Asp224Alafs*43) in the LDLR and a known single nucleotide variant (c.9835A > G, p.(Ser3279Gly) in the APOB gene. It is thought that the LDLR variant causes a protein to be prematurely truncated, likely through nonsense-mediated protein decay. The LDLR variant is strongly predicted to be pathogenic in accordance with ACMG guidelines and co-segregated with the FH clinical characteristics of the family. This LDLR variant exhibited severe clinical FH phenotypes and was restricted to the LDLR protein’s ligand-binding domain. According to computational functional characterization, this LDLR variant was predicted to change the free energy dynamics of the RNA molecule, thereby affecting its stability. This frameshift variant is thought to eliminate important functional domains in LDLR that are required for receptor recycling and LDL particle binding. We provide insight into how FH patients with a null variant in the LDLR gene respond to lipid-lowering therapy. Conclusions: The findings expand the range of FH variants and assist coronary artery disease preventive efforts by improving diagnosis, understanding the genotype-phenotype relationship, prognosis, and personalised therapy for patients with FH.
Wear Performance of Cu–Cd, Cu–Be and Cu–Cr–Zr Spot Welding Electrode Materials
Authors: Bachchhav Bhanudas D. , Chaitanya Shrikant V. , Salunkhe Sachin , Chandrakumar Palanisamy , Pagáč Marek , Nasr Emad Abouel
Published: July 2023

Heating of the electrode at the work–piece interface zone in spot welding, leading to degradation of the tip, becomes a significant concern in the high-volume production automotive industry. By recognizing the interrelationship between hardness, wear resistance, and thermal conductivity, the authors emphasize the importance of selecting electrode materials with suitable alloying elements desirable for achieving optimal performance in spot welding applications. This paper studies the wear behaviour of three types of spot-welding electrode materials under dry sliding contact conditions. A pin-on-disc tester was used to investigate Cu–Cd, Cu–Be and Cu–Cr–Zr alloys’ wear behaviour under variable parametric load, temperature and time conditions. Taguchi L9 orthogonal array was used to investigate the significance of parameters and their effect on linear wear. The ranking of the parameters was performed using SN ratio analysis. The wear mechanism was also studied using SEM analysis. Abrasive wear was observed at lower loads, while adhesion, oxidation and plastic deformation were observed under high-load and -temperature conditions. This study suggests an alternative to the presently used electrolytic tough pitch (ETP) Cu electrode involving equally good wear-resistance material. However, a detailed investigation on the effect of plasma on the metallurgical characteristics of selected material is suggested.
Current Knowledge on Friction, Lubrication, and Wear of Ethanol-Fuelled Engines—A Review
Authors: Costa Henara Lillian , Cousseau Tiago , Souza Roberto Martins
Published: July 2023

The urgent need for drastic reduction in emissions due to global warming demands a radical energy transition in transportation. The role of biofuels is fundamental to bridging the current situation towards a clean and sustainable future. In passenger cars, the use of ethanol fuel reduces gas emissions (CO2 and other harmful gases), but can bring tribological challenges to the engine. This review addresses the current state-of-the-art on the effects of ethanol fuel on friction, lubrication, and wear in car engines, and identifies knowledge gaps and trends in lubricants for ethanol-fuelled engines. This review shows that ethanol affects friction and wear in many ways, for example, by reducing lubricant viscosity, which on the one hand can reduce shear losses under full film lubrication, but on the other can increase asperity contact under mixed lubrication. Therefore, ethanol can either reduce or increase engine friction depending on the driving conditions, engine temperature, amount of diluted ethanol in the lubricant, lubricant type, etc. Ethanol increases corrosion and affects tribocorrosion, with significant effects on engine wear. Moreover, ethanol strongly interacts with the lubricant’s additives, affecting friction and wear under boundary lubrication conditions. Regarding the anti-wear additive ZDDP, ethanol leads to thinner tribofilms with modified chemical structure, in particular shorter phosphates and increased amount of iron sulphides and oxides, thereby reducing their anti-wear protection. Tribofilms formed from Mo-DTC friction modifier are affected as well, compromising the formation of low-friction MoS2 tribofilms; however, ethanol is beneficial for the tribological behaviour of organic friction modifiers. Although the oil industry has implemented small changes in oil formulation to ensure the proper operation of ethanol-fuelled engines, there is a lack of research aiming to optimize lubricant formulation to maximize ethanol-fuelled engine performance. The findings of this review should shed light towards improved oil formulation as well as on the selection of materials and surface engineering techniques to mitigate the most pressing problems.
An Armour Structure to Suppress the Brittle Failure of Ceramic Coatings
Authors: Liu Wei , Bao Fubing , Zhang Yinning , Wang Jinqing , Liang Xiaoyu
Published: July 2023

The brittle failure of ceramic coatings limits their application in many fields. To address this issue, a novel armoured ceramic coating was developed to suppress brittle failure. First, an interconnected frame microstructure was micromachined onto the surface of a mild steel substrate using a nanosecond laser. Subsequently, a polymer-derived ceramic slurry was sprayed and sintered to obtain an armoured ceramic coating. The laser-micromachined burr-like microstructure of the substrate facilitated adhesion between the coating and the substrate. The results of the mechanical properties test showed that the armoured coating could withstand more than 20 cycles of water-cooled thermal shock at 600 °C, and the peeling area of the armoured coating was approximately three times less than that of the unarmoured coating under a normal load of 1471 N. The laboratory and field corrosion test results indicated that at high temperatures, the corrosion resistance of the armoured coating was comparable with that of the unarmoured coating and was approximately 10 times higher than that of the uncoated sample. The proposed method will aid in suppressing the brittle failure of ceramic coatings and broaden their scope of application in different fields.
Synthesis and Characterization of Zeolite NaY Dispersed on Bamboo Wood
Authors: Tawachkultanadilok Pimrapus , Osakoo Nattawut , Keawkumay Chalermpan , Deekamwong Krittanun , Sosa Narongrit , Rojviriya Catleya , Nijpanich Supinya , Chanlek Narong , Prayoonpokarach Sanchai , Wittayakun Jatuporn
Published: July 2023

Zeolites in powder form have the potential to agglomerate, lowering access to active sites. Furthermore, a suspension of fine zeolite powder in liquid media is difficult to separate. Such drawbacks could be improved by dispersing zeolite crystals on support materials. This work demonstrates the dispersion of zeolite NaY crystals on bamboo wood by mixing the wood with zeolite gel before hydrothermal treatment. The syntheses were performed with acid-refluxed and non–refluxed wood. The phase of zeolites, particle distribution and morphology, zeolite content in the wood, and zeolite–wood interaction were investigated using X-ray diffraction, X-ray tomography, scanning electron microscopy, thermogravimetric analysis, nitrogen sorption analysis, and X-ray photoelectron spectroscopy. Higher zeolite content and better particle dispersion were obtained in the synthesis with the acid–refluxed wood. The composite of NaY on the acid-refluxed wood was demonstrated to be an effective adsorbent for Ni(II) ions in aqueous solutions, providing a higher adsorbed amount of Ni(II) per weight of NaY.
Thermal Stress Formation in a Functionally Graded Al2O3-Adhesive Single Lap Joint Subjected to a Uniform Temperature Field
Authors: Apalak Mustafa Kemal , Reddy Junuthula N.
Published: July 2023

This study investigates the strain and stress states in an aluminum single lap joint bonded with a functionally graded Al2O3 micro particle reinforced adhesive layer subjected to a uniform temperature field. Navier equations of elasticity theory were designated by considering the spatial derivatives of Lamé constants and the coefficient of thermal expansion for local material composition. The set of partial differential equations and mechanical boundary conditions for a two-dimensional model was reduced to a set of linear equations by means of the central finite difference approximation at each grid point of a discretized joint. The through-thickness Al2O3-adhesive composition was tailored by the functional grading concept, and the mechanical and thermal properties of local adhesive composition were predicted by Mori–Tanaka’s homogenization approach. The adherend–adhesive interfaces exhibited sharp discontinuous thermal stresses, whereas the discontinuous nature of thermal strains along bi-material interfaces can be moderated by the gradient power index, which controls the through-thickness variation of particle amount in the local adhesive composition. The free edges of the adhesive layer were also critical due to the occurrence of high normal and shear strains and stresses. The gradient power index can influence the distribution and levels of strain and stress components only for a sufficiently high volume fraction of particles. The grading direction of particles in the adhesive layer was not influential because the temperature field is uniform; namely, it can only upturn the low and high strain and stress regions so that the neat adhesive–adherend interface and the particle-rich adhesive–adherend interface can be relocated.
A Model of Triage of Serious Spinal Pathologies and Therapeutic Options Based on a Delphi Study
Authors: Meidinger Philippe , Kéchichian Amélie , Pinsault Nicolas
Published: July 2023

Background and Objectives: The relevance of red flags in serious spinal pathology (SSP) has evolved throughout the last years. Recently, new considerations have been proposed to expand the consideration of red flags. The purpose of this study was to determine, approve and test a model for the triage and management process of SSPs based on the latest data available in the literature. Materials and Methods: The SSP model was initially built on the basis of a literature review. The model was further determined and approved by an expert panel using a Delphi process. Finally, clinical scenarios were used to test the applicability of the model. Results: After three rounds of the Delphi process, panellists reached a consensus on a final version of the model. The use of clinical scenarios by experts brought about reflexive elements both for the determined model and for the SSPs depicted in the clinical cases. Conclusions: The validation of the model and its implementation in the clinical field could help assess the skills of first-line practitioners managing spinal pain patients. To this end, the development of additional clinical scenarios fitting the determined model should be further considered.
Pre-Treatment and Post-Treatment Dental Anxiety in Patients Visiting Intern Dental Clinic
Authors: Alsakr Abdulaziz , Gufran Khalid , Alqahtani Abdullah Saad , Alkharaan Hassan , Abushanan Alwaleed , Alnufaiy Banna , Alkhaldi Abdullah , Alshammari Tareq , Alanazi Muhannad
Published: July 2023

Background and Objectives: This study aimed to assess the dental anxiety of patients using the modified dental anxiety scale (MDAS) questionnaire along with examining the possible relationship between dental anxiety and sociodemographic factors. Materials and Methods: The MDAS questionnaire was used to assess the anxiety level of the patients which included a total of five questions and five options to respond to each question. MDAS questionnaire was filled out by all the patients before the dental treatment. After finishing the treatment, all the patients were given a post-treatment questionnaire to assess their anxiety levels after treatment. Descriptive statistics were performed for measuring the frequency of responses. Mann-Whitney U test was used to compare the anxiety between the gender. The chi-square test was used to identify the distribution of pre-treatment with gender and post-treatment questionnaire with gender and treatment modalities. Linear regression was used to identify the association between clinical variables and different levels of anxiety. Results: A total of 115 patients including 59 males and 56 females with a mean age of 35 ± 11.34 years were included in the current study. Female patients were significantly more anxious about dental treatment compared to male patients. Linear regression showed that age and gender have a significant association with the pre-treatment anxiety level; however, types of treatment is not associated with the MDAS. Anxiety levels decreased for the majority of the patients after the treatment and types of treatment did not show any differences with the post-treatment anxiety level. Conclusions: Age and gender play an important role in dental anxiety; however, types of treatment are not associated with pre-treatment and post-treatment dental anxiety.
Automated Liquid–Liquid Displacement Porometry (LLDP) for the Non-Destructive Characterization of Ultrapure Water Purification Filtration Devices
Authors: Peinador René I. , Darbouret Daniel , Paragot Christophe , Calvo José I.
Published: July 2023

This scientific publication presents a novel modification of the liquid–liquid displacement porosimetry (LLDP) method, aiming for the non-destructive automated analysis of water purification membrane filtration devices in the microfiltration (MF) and ultrafiltration (UF) range. The technical adaptation of LLDP enables the direct in-line porosimetric analysis of commercial filtration devices, avoiding the filtration devices’ destruction. Six commercially available filtration devices with polyethersulfone (PES) and polysulfone (PS) membranes were studied using an improved device developed by the IFTS, which was based on a commercial LLDP instrument. The filtration devices were evaluated in three different configurations: flat disks, hollow fibers, and pleated membranes. The results obtained using the proposed method were compared with other characterization techniques, including submicronic efficiency retention, image analysis of scanning electron microscopy (SEM), and gas–liquid displacement porosimetry (GLDP). The comparison of the results demonstrated that the proposed method accurately determined the porosimetric characteristics of the filters. It proved to be a precise technique for the non-destructive in-line evaluation of filter performance, as well as for periodic quality control and the fouling degree assessment of commercial filtration devices. This modified LLDP approach offers significant potential for the advanced characterization and quality assessment of water purification membrane filtration devices, contributing to improved understanding and optimization of their performance.
Development of Nano–Micro Fused LSPR Chip for In Situ Single-Cell Secretion Analysis
Authors: Terada Yuhei , Obara Ain , Briones Jonathan Campos , Luo Xi , Espulgar Wilfred Villariza , Saito Masato , Takamatsu Hyota , Tamiya Eiichi
Published: July 2023

Single-cell analysis has become increasingly important in uncovering cell heterogeneity, which has great implications in medicine and biology for a deep understanding of cell characteristics. Owing to its significance, it is vital to create novel devices that can reveal special or unique cells. In this work, we developed a single-cell secretion detection chip consisting of microwells that can trap single cells. Each well is surrounded by Au nanopillars capable of localized surface plasmon resonance (LSPR) measurement. Using microfabrication and nanofabrication techniques, Au nanopillar and microwell structures were fabricated on a COP film. The Au nanopillar was modified with IL-6 antibodies for the direct detection of single-cell secreted IL-6 via LSPR absorbance peak shift. Specific IL-6 detection was successfully demonstrated using a null and IL-6 oversecreting Jurkat cell. A high single-cell trapping efficiency of over 80% was also achieved. Overall, the development of this single-cell secretion detection chip with a simple LSPR measurement setup represents a significant development in the field of cell biology and immunology, providing researchers with a powerful tool for studying individual cells and their secreted cytokines, and is useful for point-of-care testing (POCT) diagnostics.
Multiple Biological Mechanisms for the Potential Influence of Phytochemicals on Physical Activity Performance: A Narrative Review
Authors: Thomas Robert , Williams Madeleine , Aldous Jeffrey , Wyld Kevin
Published: July 2023

Natural phytochemicals (PCs) are responsible for the taste, colour, and aroma of many edible plants. Cohort studies have linked higher intake to a reduced risk of chronic degenerative diseases and premature ageing. The ability of foods rich in PCs, such as phytanthocyanins, apigenin, flavonols, flavonoids, bioflavonoids, gallic acid, ellagic acid, quercetin, and ellagitannins, to support physical activity has also been highlighted in a number of published pre-clinical and prospective clinical studies. This literature mostly emphasises the ability of PCs to enhance the adaptive upregulation of antioxidant enzymes (AEs), which reduces exercise-associated oxidative stress, but there are several other mechanisms of benefit that this narrative review addresses. These mechanisms include; protecting joints and tendons from physical trauma during exercise; mitigating delayed-onset muscle symptoms (DOMS) and muscle damage; improving muscle and tissue oxygenation during training; cultivating a healthy gut microbiome hence lowering excess inflammation; cutting the incidence of upper respiratory tract viral infections which disrupt training programmes; and helping to restore circadian rhythm which improves sleep recovery and reduces daytime fatigue, which in turn elevates mood and motivation to train.
The Potential of Probiotics as Ingestible Adjuvants and Immune Modulators for Antiviral Immunity and Management of SARS-CoV-2 Infection and COVID-19
Authors: Tomkinson Sophie , Triscott Cloe , Schenk Emily , Foey Andrew
Published: July 2023

Probiotic bacteria are able to modulate general antiviral responsiveness, including barrier functionality and innate and adaptive immune responses. The COVID-19 pandemic, resulting from SARS-CoV-2 infection, has created a need to control and treat this viral infection and its ensuing immunopathology with a variety of approaches; one such approach may involve the administration of probiotic bacteria. As with most viral infections, its pathological responses are not fully driven by the virus, but are significantly contributed to by the host’s immune response to viral infection. The potential adoption of probiotics in the treatment of COVID-19 will have to appreciate the fine line between inducing antiviral immunity without over-provoking immune inflammatory responses resulting in host-derived immunopathological tissue damage. Additionally, the effect exerted on the immune system by SARS-CoV-2 evasion strategies will also have to be considered when developing a robust response to this virus. This review will introduce the immunopathology of COVID-19 and the immunomodulatory effects of probiotic strains, and through their effects on a range of respiratory pathogens (IAV, SARS-CoV, RSV), as well as SARS-CoV-2, will culminate in a focus on how these bacteria can potentially manipulate both infectivity and immune responsiveness via barrier functionality and both innate and adaptive immunity. In conclusion, the harnessing of induction and augmentation of antiviral immunity via probiotics may not only act as an ingestible adjuvant, boosting immune responsiveness to SARS-CoV-2 infection at the level of barrier integrity and innate and adaptive immunity, but also act prophylactically to prevent infection and enhance protection afforded by current vaccine regimens.
Genomic Characterization of Fecal Escherichia coli Isolates with Reduced Susceptibility to Beta-Lactam Antimicrobials from Wild Hogs and Coyotes
Authors: Awosile Babafela , Fritzler Jason , Levent Gizem , Rahman Md. Kaisar , Ajulo Samuel , Daniel Ian , Tasnim Yamima , Sarkar Sumon
Published: July 2023

This study was carried out to determine the antimicrobial resistance (AMR) genes and mobile genetic elements of 16 Escherichia coli isolates—with reduced susceptibility to ceftazidime and imipenem—that were recovered from the fecal samples of coyotes and wild hogs from West Texas, USA. Whole-genome sequencing data analyses revealed distinct isolates with a unique sequence type and serotype designation. Among 16 isolates, 4 isolates were multidrug resistant, and 5 isolates harbored at least 1 beta-lactamase gene (blaCMY-2, blaCTX-M-55, or blaCTX-M-27) that confers resistance to beta-lactam antimicrobials. Several isolates carried genes conferring resistance to tetracyclines (tet(A), tet(B), and tet(C)), aminoglycosides (aac(3)-IId, ant(3″)-Ia, aph(3′)-Ia, aph(3″)-lb, aadA5, and aph(6)-ld), sulfonamides (sul1, sul2, and sul3), amphenicol (floR), trimethoprim (dfrA1 and dfrA17), and macrolide, lincosamide, and streptogramin B (MLSB) agents (Inu(F), erm(B), and mph(A)). Nine isolates showed chromosomal mutations in the promoter region G of ampC beta-lactamase gene, while three isolates showed mutations in gyrA, parC, and parE quinolone resistance-determining regions, which confer resistance to quinolones. We also detected seven incompatibility plasmid groups, with incF being the most common. Different types of virulence genes were detected, including those that enhance bacterial fitness and pathogenicity. One blaCMY-2 positive isolate (O8:H28) from a wild hog was also a Shiga toxin-producing E. coli and was a carrier of the stx2A virulence toxin subtype. We report the detection of blaCMY-2, blaCTX-M-55, and blaCTX-M-27 beta-lactamase genes in E. coli from coyotes for the first time. This study demonstrates the importance of wildlife as reservoirs of important multi-drug-resistant bacteria and provides information for future comparative genomic analysis with the limited literature on antimicrobial resistance dynamics in wildlife such as coyotes.
DNA Repair Inhibitors: Potential Targets and Partners for Targeted Radionuclide Therapy
Authors: Obata Honoka , Ogawa Mikako , Zalutsky Michael R.
Published: July 2023

The present review aims to explore the potential targets/partners for future targeted radionuclide therapy (TRT) strategies, wherein cancer cells often are not killed effectively, despite receiving a high average tumor radiation dose. Here, we shall discuss the key factors in the cancer genome, especially those related to DNA damage response/repair and maintenance systems for escaping cell death in cancer cells. To overcome the current limitations of TRT effectiveness due to radiation/drug-tolerant cells and tumor heterogeneity, and to make TRT more effective, we propose that a promising strategy would be to target the DNA maintenance factors that are crucial for cancer survival. Considering their cancer-specific DNA damage response/repair ability and dysregulated transcription/epigenetic system, key factors such as PARP, ATM/ATR, amplified/overexpressed transcription factors, and DNA methyltransferases have the potential to be molecular targets for Auger electron therapy; moreover, their inhibition by non-radioactive molecules could be a partnering component for enhancing the therapeutic response of TRT.
Virus-Based Biological Systems as Next-Generation Carriers for the Therapy of Central Nervous System Diseases
Authors: Nowak Ilona , Madej Marcel , Secemska Julia , Sarna Robert , Strzalka-Mrozik Barbara
Published: July 2023

Central nervous system (CNS) diseases are currently a major challenge in medicine. One reason is the presence of the blood–brain barrier, which is a significant limitation for currently used medicinal substances that are characterized by a high molecular weight and a short half-life. Despite the application of nanotechnology, there is still the problem of targeting and the occurrence of systemic toxicity. Viral vectors and virus-like particles (VLPs) may provide a promising solution to these challenges. Their small size, biocompatibility, ability to carry medicinal substances, and specific targeting of neural cells make them useful in research when formulating a new generation of biological carriers. Additionally, the possibility of genetic modification has the potential for gene therapy. Among the most promising viral vectors are adeno-associated viruses, adenoviruses, and retroviruses. This is due to their natural tropism to neural cells, as well as the possibility of genetic and surface modification. Moreover, VLPs that are devoid of infectious genetic material in favor of increasing capacity are also leading the way for research on new drug delivery systems. The aim of this study is to review the most recent reports on the use of viral vectors and VLPs in the treatment of selected CNS diseases.
Hierarchical Feature Enhancement Algorithm for Multispectral Infrared Images of Dark and Weak Targets
Authors: Yang Shuai , Zou Zhihui , Li Yingchao , Shi Haodong , Fu Qiang
Published: July 2023

A multispectral infrared zoom optical system design and a single-frame hierarchical guided filtering image enhancement algorithm are proposed to address the technical problems of low contrast, blurred edges, and weak signal strength of single-spectrum infrared imaging of faint targets, which are easily drowned out by noise. The multispectral infrared zoom optical system, based on the theory of complex achromatic and mechanical positive group compensation, can simultaneously acquire multispectral image information for faint targets. The single-frame hierarchical guided filtering image enhancement algorithm, which extracts the background features and detailed features of faint targets in a hierarchical manner and then weights fusion, effectively enhances the target and suppresses the interference of complex background and noise. Solving multi-frame processing increases data storage and real-time challenges. The experimental verification of the optical system design and image enhancement algorithm proposed in this paper separately verified that the experimental enhancement was significant, with the combined use improving Mean Square Error (MSE) by 14.32, Signal-Noise Ratio (SNR) by 11.64, Peak Signal-to-Noise Ratio (PSNR) by 12.78, and Structural Similarity (SSIM) by 14.0% compared to guided filtering. This research lays the theoretical foundation for the research of infrared detection and tracking technology for clusters of faint targets.
The Key Role of Glutamate Dehydrogenase 2 (GDH2) in the Control of Kernel Production in Maize (Zea mays L.)
Authors: Tercé-Laforgue Thérèse , Lothier Jérémy , Limami Anis M. , Rouster Jacques , Lea Peter J. , Hirel Bertrand
Published: July 2023

The agronomic potential of glutamate dehydrogenase 2 (GDH2) in maize kernel production was investigated by examining the impact of a mutation on the corresponding gene. Mu-insertion homozygous and heterozygous mutant lines lacking GDH2 activity were isolated and characterized at the biochemical, physiological and agronomic levels. In comparison to the wild type and to the homozygous ghd2 mutants, the heterozygous gdh2 mutant plants were characterized by a decrease in the root amino acid content, whereas in the leaves an increase of a number of phenolic compounds was observed. On average, a 30 to 40% increase in kernel yield was obtained only in the heterozygous gdh2 mutant lines when plants were grown in the field over two years. The importance of GDH2 in the control of plant productivity is discussed in relation to the physiological impact of the mutation on amino acid content, with primary carbon metabolism mostly occurring in the roots and secondary metabolism occurring in the leaves.
Pedicularis rostratospicata subsp. marsica (P. Sect. Rostratae, Orobanchaceae), a New Subspecies from the Central Apennines (Italy)
Authors: Conti Fabio , Oberprieler Christoph , Dorfner Marco , Schabel Erik , Bartolucci Fabrizio
Published: July 2023

The new subspecies Pedicularis rostratospicata subsp. marsica is here described based on morphological and molecular analyses. The new taxon is endemic to few localities of the Central Apennines within the Abruzzo, Lazio and Molise National Park (Central Italy). Pedicularis rostratospicata subsp. marsica can be distinguished from the other two currently accepted subspecies (subsp. rostratospicata and subsp. helvetica) by their taller stems, shorter petioles of basal and middle leaves, narrower blades of basal leaves, longer inflorescences with longer internodes and more flowers, and longer calyx lobes. Molecular analysis confirmed the autonomy of the new taxon. Furthermore, the conservation status assessment of the new subspecies according to IUCN categories and criteria is proposed and discussed, and an analytical key to the three subspecies of P. rostratospicata is presented.
Constituents and Selective BuChE Inhibitory Activity of the Essential Oil from Hypericum aciculare Kunth
Authors: Calva James , Ludeña Carlos , Bec Nicole , Larroque Christian , Salinas Melissa , Vidari Giovanni , Armijos Chabaco
Published: July 2023

A potential source of new inhibitors of cholinesterase enzymes are certain compounds of natural plant origin; therefore, in the study described herein we have determined the chemical composition and the acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) inhibitory activities of the essential oil (EO) steam distilled from aerial parts of Hypericum aciculare, which was collected in southern Ecuador. The oil qualitative and quantitative composition was determined by GC-FID and GC-MS using a non-polar and a polar chromatographic column. A total of fifty-three constituents were identified, that accounted for about 98% of the EO content. The hydrocarbon n-nonane (16.4–28.7%) and the aldehyde n-decanal (20.7–23.1%) were the predominant oil constituents. In addition, the EO showed significant inhibition of BuChE (IC50 = 28.3 ± 2.7 μg/mL) and moderate activity towards AChE (IC50 = 82.1 ± 12.1 µg/mL). Thus, the EO from H. aciculare aerial parts is an interesting candidate to investigate the mechanism of selective ChE inhibition by the two ChE enzymes with the aim to discover potential targets to control the progression of the Alzheimer’s disease (AD).
Effects of Different Polypropylene (PP)-Backbones in Aluminium Feedstock for Fused Filament Fabrication (FFF)
Authors: Momeni Vahid , Shahroodi Zahra , Gonzalez-Gutierrez Joamin , Hentschel Lukas , Duretek Ivica , Schuschnigg Stephan , Kukla Christian , Holzer Clemens
Published: July 2023

The current study presents the effect of the backbone as an important binder component on the mechanical, rheological, and thermal properties of Aluminium (Al) alloy feedstocks. A thermoplastic elastomer (TPE) main binder component was blended with either polypropylene (PP), grafted-maleic anhydride-PP (PPMA), or grafted-maleic anhydride-PPwax (PPMAwax) plus PP, as the backbone. Differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA) tests were performed to investigate the thermal properties of binder systems and feedstocks. Fourier-transform infrared (FTIR) spectroscopy was used to study the chemical interaction between the binder and the Al alloy. After making feedstock filaments, tensile tests, scanning electron microscopy (SEM), and fused filament fabrication (FFF) printing were done. The results showed that although the PP printability was acceptable, the best mechanical properties and printed quality can be achieved by PPMA. TGA test showed that all binder systems in the feedstocks could be removed completely around 500 °C. From FTIR, the possibility of chemical reactions between Al alloy particles and maleic anhydride groups on the grafted PP backbone could explain the better dispersion of the mixture and higher mechanical properties. Tensile strength in PP samples was 3.4 MPa which was improved 1.8 times using PPMA as the backbone.