bluuebunny/crossref_metadata_2025 huggingface dataset:
Microalgae Cultivation on Anaerobic Digestate of Municipal Wastewater, Sewage Sludge and Agro-Waste
Authors: Zuliani Luca , Frison Nicola , Jelic Aleksandra , Fatone Francesco , Bolzonella David , Ballottari Matteo
Published: October 2016

Microalgae are fast-growing photosynthetic organisms which have the potential to be exploited as an alternative source of liquid fuels to meet growing global energy demand. The cultivation of microalgae, however, still needs to be improved in order to reduce the cost of the biomass produced. Among the major costs encountered for algal cultivation are the costs for nutrients such as CO2, nitrogen and phosphorous. In this work, therefore, different microalgal strains were cultivated using as nutrient sources three different anaerobic digestates deriving from municipal wastewater, sewage sludge or agro-waste treatment plants. In particular, anaerobic digestates deriving from agro-waste or sewage sludge treatment induced a more than 300% increase in lipid production per volume in Chlorella vulgaris cultures grown in a closed photobioreactor, and a strong increase in carotenoid accumulation in different microalgae species. Conversely, a digestate originating from a pilot scale anaerobic upflow sludge blanket (UASB) was used to increase biomass production when added to an artificial nutrient-supplemented medium. The results herein demonstrate the possibility of improving biomass accumulation or lipid production using different anaerobic digestates.
Molecular Characterization, Tissue Distribution and Expression, and Potential Antiviral Effects of TRIM32 in the Common Carp (Cyprinus carpio)
Authors: Wang Yeda , Li Zeming , Lu Yuanan , Hu Guangfu , Lin Li , Zeng Lingbing , Zhou Yong , Liu Xueqin
Published: October 2016

Tripartite motif-containing protein 32 (TRIM32) belongs to the tripartite motif (TRIM) family, which consists of a large number of proteins containing a RING (Really Interesting New Gene) domain, one or two B-box domains, and coiled coil motif followed by different C-terminal domains. The TRIM family is known to be implicated in multiple cellular functions, including antiviral activity. However, it is presently unknown whether TRIM32 of common carp (Cyprinus carpio) has the antiviral effect. In this study, the sequence, expression, and antiviral function of TRIM32 homolog from common carp were analyzed. The full-length coding sequence region of trim32 was cloned from common carp. The results showed that the expression of TRIM32 (mRNA) was highest in the brain, remained stably expressed during embryonic development, and significantly increased following spring viraemia of carp virus (SVCV) infection. Transient overexpression of TRIM32 in affected Epithelioma papulosum cyprinid cells led to significant decrease of SVCV production as compared to the control group. These results suggested a potentially important role of common carp TRIM32 in enhancing host immune response during SVCV infection both in vivo and in vitro.
Gelation Behaviors and Mechanism of Silk Fibroin According to the Addition of Nitrate Salts
Authors: Im Dong , Kim Min , Yoon Young , Park Won
Published: October 2016

Silk fibroin (SF) is a typical fibrous protein that is secreted by silkworms and spiders. It has been used in a variety of areas, and especially for tissue-engineering scaffolds, due to its sound processability, mechanical properties, biodegradability, and biocompatibility. With respect to gelation, the SF gelation time is long in aqueous solutions, so a novel approach is needed to shorten this time. The solubility of regenerated SF is sound in formic acid (FA), which is a carboxylic acid of the simplest structure. In this study, SF was dissolved in formic acid, and the addition of salts then induced a rapid gelation that accompanied a solution-color change. Based on the gelation behaviors of the SF solution according to different SF and salt concentrations, the gelation mechanism was investigated.
Racemization of the Succinimide Intermediate Formed in Proteins and Peptides: A Computational Study of the Mechanism Catalyzed by Dihydrogen Phosphate Ion
Authors: Takahashi Ohgi , Kirikoshi Ryota , Manabe Noriyoshi
Published: October 2016

In proteins and peptides, d-aspartic acid (d-Asp) and d-β-Asp residues can be spontaneously formed via racemization of the succinimide intermediate formed from l-Asp and l-asparagine (l-Asn) residues. These biologically uncommon amino acid residues are known to have relevance to aging and pathologies. Although nonenzymatic, the succinimide racemization will not occur without a catalyst at room or biological temperature. In the present study, we computationally investigated the mechanism of succinimide racemization catalyzed by dihydrogen phosphate ion, H2PO4−, by B3LYP/6-31+G(d,p) density functional theory calculations, using a model compound in which an aminosuccinyl (Asu) residue is capped with acetyl (Ace) and NCH3 (Nme) groups on the N- and C-termini, respectively (Ace–Asu–Nme). It was shown that an H2PO4− ion can catalyze the enolization of the Hα–Cα–C=O portion of the Asu residue by acting as a proton-transfer mediator. The resulting complex between the enol form and H2PO4− corresponds to a very flat intermediate region on the potential energy surface lying between the initial reactant complex and its mirror-image geometry. The calculated activation barrier (18.8 kcal·mol−1 after corrections for the zero-point energy and the Gibbs energy of hydration) for the enolization was consistent with the experimental activation energies of Asp racemization.
Life Cycle Cost Analysis of Three Types of Power Lines in 10 kV Distribution Network
Authors: Zhu Zhenyu , Lu Siyao , Gao Bingtuan , Yi Tao , Chen Bin
Published: October 2016

There are three types of power lines in the 10 kV distribution network in China, i.e., copper power cables, overhead power conductors and aluminum alloy power cables. It is necessary to give a comprehensive evaluation to choose the type of power line in some delicate practical engineering. This paper presents a life cycle cost (LCC)-based analysis method for the three types of power lines. An LCC model of the power line in the 10 kV distribution network is established, which considers four parts: investment cost, operation and maintenance cost, failure cost and discard cost. A detailed calculation model for the four parts is presented, and to calculate the failure cost, the Monte Carlo algorithm is employed to simulate the values of expected energy not supplied (EENS). Two practical 10 kV power line projects in Fujian Province in China were analyzed based on the proposed LLC model and corresponding developed software, which has helped the power company select the appropriate power line successfully.
The Influence of Parental Emotional Neglect on Assault Victims Seeking Treatment for Depressed Mood and Alcohol Misuse: A Pilot Study
Authors: Bailey Kylie , Baker Amanda , McElduff Patrick , Kavanagh David
Published: October 2016

This study explores the relationship between reported parental emotional neglect when a child, assault type experienced, posttraumatic stress symptoms (PTSS), depression, and alcohol consumption in treatment seekers for comorbid depressive symptoms and alcohol misuse. Participants (n = 220) with concurrent depression and alcohol misuse were recruited from the DAISI (Depression and Alcohol Integrated and Single-focussed Interventions) project. Assault type and PTSS were retrospectively assessed by the Posttraumatic Stress Diagnostic Scale. The Measure of Parenting Style is a self-report measure that retrospectively assessed emotional neglect experienced as a child. An exploratory factor analysis using the tetrachoric correlation matrix (applying principal factor extraction with a varimax rotation) identified the two assault factors of sexual assault (SA) and physical assault (PA). A path analysis revealed that Maternal Emotional Neglect increased the impact of PTSS and depression. Paternal Emotional Neglect increased the impact of PA on PTSS and alcohol dependence symptoms. There appears to be differential effects of assault type and Maternal/Paternal emotional neglect on depression and alcohol misuse, suggesting that parenting roles serve distinct protective functions.
FLEHAP: A Wind Powered Supply for Autonomous Sensor Nodes
Authors: Boccalero Gregorio , Boragno Corrado , Caviglia Daniele , Morasso Remy
Published: October 2016

The development of the Internet of Things infrastructure requires the deployment of millions of heterogeneous sensors embedded in the environment. The powering of these sensors cannot be done with wired connections, and the use of batteries is often impracticable. Energy harvesting is the common proposed solution, and many devices have been developed for this purpose, using light, mechanical vibrations, and temperature differences as energetic sources. In this paper we present a novel energy-harvester device able to capture the kinetic energy from a fluid in motion and transform it in electrical energy. This device, named FLEHAP (FLuttering Energy Harvester for Autonomous Powering), is based on an aeroelastic effect, named fluttering, in which a totally passive airfoil shows large and regular self-sustained motions (limit cycle oscillations) even in extreme conditions (low Reynolds numbers), thanks to its peculiar mechanical configuration. This system shows, in some centimeter-sized configurations, an electrical conversion efficiency that exceeds 8% at low wind speed (3.5 m/s). By using a specialized electronic circuit, it is possible to store the electrical energy in a super capacitor, and so guarantee self-powering in such environmental conditions.
Magnetic Behavior of Carboxylate and β-Diketonate Lanthanide Complexes Containing Stable Organometallic Moieties in the Core-Forming Ligand
Authors: Efimov Nikolay , Koroteev Pavel , Gavrikov Andrey , Ilyukhin Andrey , Dobrokhotova Zhanna , Novotortsev Vladimir
Published: October 2016

Information concerning the structures of compounds of rare earth elements with carboxylic acids and a β-diketone containing stable organometallic moieties that we obtained previously is presented. Additional results for 15 complexes with the [Gd2O2] core allowed confirming and improving the correlation between JGd–Gd′ and the Gd…Gd distance for complexes of this type that we found earlier. For the first time, dc and ac magnetic measurements were carried out for the formerly-described complex [Dy2(O2CCym)4(NO3)2(DMSO)4] (2), Cym = (η5-C5H4)Mn(CO)3), and two new binuclear complexes, namely [Dy2(O2CFc)4(NO3)2(DMSO)4] (3), and [Dy2(O2CFc)6(DMSO)2(H2O)2] (4), Fc = (η5-C5H4)Fe(η5-C5H5)). For binuclear [Dy2(O2CCym)6(DMSO)4] (1), as well as for a 1D-polymer [Dy(O2CCym)(acac)2(H2O)]n (6), ac magnetic measurements were carried out more precisely. The characteristics of a single-molecule magnet and of a single-chain magnet were determined for Complexes 2 and 6, respectively.
The Effects of Pulse Parameters on Weld Geometry and Microstructure of a Pulsed Laser Welding Ni-Base Alloy Thin Sheet with Filler Wire
Authors: Chai Dongsheng , Wu Dongdong , Ma Guangyi , Zhou Siyu , Jin Zhuji , Wu Dongjiang
Published: October 2016

Due to its excellent resistance to corrosive environments and its superior mechanical properties, the Ni-based Hastelloy C-276 alloy was chosen as the material of the stator and rotor cans of a nuclear main pump. In the present work, the Hastelloy C-276 thin sheet 0.5 mm in thickness was welded with filler wire by a pulsed laser. The results indicated that the weld pool geometry and microstructure were significantly affected by the duty ratio, which was determined by the pulse duration and repetition rate under a certain heat input. The fusion zone area was mainly affected by the duty ratio, and the relationship was given by a quadratic polynomial equation. The increase in the duty ratio coarsened the grain size, but did not obviously affect microhardness. The weld geometry and base metal dilution rate was manipulated by controlling pulsed parameters without causing significant change to the performance of the weld. However, it should be noted that, with a larger duty ratio, the partial molten zone is a potential weakness of the weld.
On the Step Cooling Treatment for the Assessment of Temper Embrittlement Susceptibility of Heavy Forgings in Superclean Steels
Authors: Roberti Roberto , Faccoli Michela
Published: October 2016

When subjected to extended exposure to intermediate service temperatures, Cr–Mo steels, Ni–Cr steels, and 5% Ni steels can become embrittled, with an associated decrease in fracture toughness and a shift in the ductile-to-brittle transition temperature to higher temperatures. Two methods for the investigation of temper embrittlement phenomena are isothermal aging or the use of a step cooling aging treatment, which is less time consuming and is considered to be the most severe test to evaluate steel’s susceptibility to this phenomenon. In the present work, the effectiveness of the step cooling treatment in the assessment of temper embrittlement in a superclean 26NiCrMoV14.5 steel for heavy section forgings has been studied. Some isothermal aging treatments in the critical temperature range have also been carried out. Results of a Charpy V impact test on not-aged and aged specimens, and observation of the fracture surfaces led to the following conclusions: the steel does not undergo temper embrittlement upon step cooling treatment or after aging at different temperatures and times in the critical temperature range; the most negative effect on the shift of the ductile-to-brittle transition curve—compared with not aged steel—has been observed after aging at 593 °C for 2 h (ΔT54J = 9 °C); further aging up to 8 h produced a ΔT54J of only 1 °C. Neither step cooling nor aging at various critical temperatures gave rise to an intergranular brittle fracture; the amount of embrittling impurity elements in a superclean steel does not seem to be enough to cause embrittlement and a pure intergranular decohesion.
Kinetics of the Carbonate Leaching for Calcium Metavanadate
Authors: Shi Peiyang , Zhang Bo , Jiang Maofa
Published: October 2016

The sodium salt roasting process was widely used for extracting vanadium due to its high yield rate of vanadium. However, the serious pollution was a problem. The calcium roasting process was environmentally friendly, but the yield rate of vanadium was relatively lower. Focusing on the calcium metavanadate produced in the calcium roasting process of vanadium minerals, the mechanism of the carbonate leaching for calcium metavanadate and its leaching kinetics of calcium metavanadate were studied. With the increase of the leaching agent content, the decrease of the particle size, the increase of the temperature and the increase of the reaction time, the leaching rate of vanadium increased, and the constant of reaction rate increased. In the carbonate leaching process, the calcium carbonate was globular and attached to the surface of calcium metavanadate. In the solution containing bicarbonate radical, lots of cracks formed in the dissolution process. However, the cracks were relatively fewer in the solution containing carbonate. In the present study, the carbonate leaching for calcium metavanadate was controlled by diffusion, the activation energy reached maximum and minimum in the sodium bicarbonate and the sodium carbonate solution, respectively. The activation energy value in the ammonium bicarbonate solution was between those two solutions. The kinetic equations of the carbonate leaching for calcium metavanadate were as follows: 1 − 2/3η − (1 − η)2/3 = 4.39[Na2CO3]0.75/r0 × exp(−2527.06/T)t; 1 − 2/3η − (1 − η)2/3 = 7.89[NaHCO3]0.53/r0 × exp(−2530.67/T)t; 1 − 2/3η − (1 − η)2/3 = 6.78[NH4HCO3]0.69/r0 × exp(−2459.71/T)t.
The Development of Fine Microgram Powder Electrode System and Its Application in the Analysis of Chalcopyrite Leaching Behavior
Authors: Miki Hajime , Hirajima Tsuyoshi , Oka Kazunori , Sasaki Keiko
Published: October 2016

An electrode system to study the mechanism of fine microgram powder sulfide mineral dissolution was developed by using a relatively simple method that enables the attachment of micrograms of fine powder to a platinum plate surface. This system yields highly reproducible results and is sensitive compared with conventional electrode systems for various sulfide minerals such as pyrite, chalcopyrite, chalcocite, enargite, and tennantite. The leaching behavior of chalcopyrite was re-examined in a test of the application of this electrode system. Chalcopyrite dissolution is enhanced in specific potential regions because it is believed to be reduced to leachable chalcocite, but this result is inconclusive because it is difficult to detect the intermediate chalcocite. Powder chalcopyrite in the new powder electrode system was held at 0.45 V in the presence of copper ion and sulfuric acid media followed by an application of potential in the anodic direction. Besides the chalcopyrite oxidation peak, a small peak resulted at ∼0.55 V; this peak corresponds to reduced chalcocite, because it occurs at the same potential as the chalcocite oxidation peak.
Nano-Tomography of Porous Geological Materials Using Focused Ion Beam-Scanning Electron Microscopy
Authors: Liu Yang , King Helen , Van Huis Marijn , Drury Martyn , Plümper Oliver
Published: October 2016

Tomographic analysis using focused ion beam-scanning electron microscopy (FIB-SEM) provides three-dimensional information about solid materials with a resolution of a few nanometres and thus bridges the gap between X-ray and transmission electron microscopic tomography techniques. This contribution serves as an introduction and overview of FIB-SEM tomography applied to porous materials. Using two different porous Earth materials, a diatomite specimen, and an experimentally produced amorphous silica layer on olivine, we discuss the experimental setup of FIB-SEM tomography. We then focus on image processing procedures, including image alignment, correction, and segmentation to finally result in a three-dimensional, quantified pore network representation of the two example materials. To each image processing step we consider potential issues, such as imaging the back of pore walls, and the generation of image artefacts through the application of processing algorithms. We conclude that there is no single image processing recipe; processing steps need to be decided on a case-by-case study.
Artemisinin and Its Derivatives as a Repurposing Anticancer Agent: What Else Do We Need to Do?
Authors: Li Zhe , Li Qin , Wu Jun , Wang Manyuan , Yu Junxian
Published: October 2016

Preclinical investigation and clinical experience have provided evidence on the potential anticancer effect of artemisinin and its derivatives (ARTs) in the recent two decades. The major mechanisms of action of ARTs may be due to toxic-free radicals generated by an endoperoxide moiety, cell cycle arrest, induction of apoptosis, and inhibition of tumor angiogenesis. It is very promising that ARTs are expected to be a new class of antitumor drugs of wide spectrum due to their detailed information regarding efficacy and safety. For developing repurposed drugs, many other characteristics of ARTs should be studied, including through further investigations on possible new pathways of anticancer effects, exploration on efficient and specific drug delivery systems-especially crossing biological barriers, and obtaining sufficient data in clinical trials. The aim of this review is to highlight these achievements and propose the potential strategies to develop ARTs as a new class of cancer therapeutic agents.
Inhibition of Uterine Contractility by Thalidomide Analogs via Phosphodiesterase-4 Inhibition and Calcium Entry Blockade
Authors: Fernández-Martínez Eduardo , Ponce-Monter Héctor , Soria-Jasso Luis , Ortiz Mario , Arias-Montaño José-Antonio , Barragán-Ramírez Guillermo , Mayén-García Cynthia
Published: October 2016

Uterine relaxation is crucial during preterm labor. Phosphodiesterase-4 (PDE-4) inhibitors have been proposed as tocolytics. Some thalidomide analogs are PDE-4 inhibitors. The aim of this study was to assess the uterus-relaxant properties of two thalidomide analogs, methyl 3-(4-nitrophthalimido)-3-(3,4-dimethoxyphenyl)-propanoate (4NO2PDPMe) and methyl 3-(4-aminophthalimido)-3-(3,4-dimethoxyphenyl)-propanoate (4APDPMe) and were compared to rolipram in functional studies of spontaneous phasic, K+-induced tonic, and Ca2+-induced contractions in isolated pregnant human myometrial tissues. The accumulation of cAMP was quantified in HeLa cells. The presence of PDE-4B2 and phosphorylated myosin light-chain (pMLC), in addition to the effect of thalidomide analogs on oxytocin-induced pMLC, were assessed in human uterine myometrial cells (UtSMCs). Thalidomide analogs had concentration-dependent inhibitory effects on spontaneous and tonic contractions and inhibited Ca2+-induced responses. Tonic contraction was equipotently inhibited by 4APDPMe and rolipram (IC50 = 125 ± 13.72 and 98.45 ± 8.86 µM, respectively). Rolipram and the thalidomide analogs inhibited spontaneous and tonic contractions equieffectively. Both analogs increased cAMP accumulation in a concentration-dependent manner (p < 0.05) and induced changes in the subcellular localization of oxytocin-induced pMLC in UtSMCs. The inhibitory effects of thalidomide analogs on the contractions of pregnant human myometrium tissue may be due to their PDE-4 inhibitory effect and novel mechanism as calcium-channel blockers.
Optimization Extraction, Preliminary Characterization and Antioxidant Activities of Polysaccharides from Semen Juglandis
Authors: Ren Xueyong , He Liang , Wang Yanbin , Cheng Junwen
Published: October 2016

The optimization extraction process, preliminary characterization and antioxidant activities of polysaccharides from Semen Juglandis (SJP) were studied in this paper. Based on the Box-Behnken experimental design and response surface methodology, the optimal extraction conditions for the SJP extraction were obtained as follows: temperature 88 °C, extraction time 125 min and ratio of liquid to solid 31 mL/g. Under these conditions, experimental extraction yield of SJP was (5.73 ± 0.014)% (n = 5), similar to the predicted value of 5.78%. Furtherly, the purified SJP obtained from SJP extract by DEAE-52 and Sephacryl S-100 chromatography was analyzed to be rhamnose, galacturonic acid, galactose, arabinose and fucose in the molar ratio of 1:6.34:1.38:3.21:1.56. And the weight-average molecular weight and radius of gyration of the purified SJP in 0.1 M NaCl were determined to be 2.76 × 104 g/mol and 122 nm by SEC-MALLS, respectively. More importantly, it exhibited appreciable antioxidant activities compared to the standard Vc, such as DPPH radical scavenging activity (IC50 0.21 mg/mL), strong reducing power, ABTS radical scavenging activity (IC50 0.29 mg/mL), and hydroxyl radical scavenging activity (IC50 0.38 mg/mL). These results indicate that SJP may be useful for developing functional health products or natural antioxidant.
Synthesis of Xylitan Derivatives and Preliminary Evaluation of in Vitro Trypanocidal Activity
Authors: Elias Paula , Coelho Gleicekelly , Xavier Viviane , Sales Junior Policarpo , Romanha Alvaro , Murta Silvane , Carneiro Claudia , Camilo Nilton , Hilário Flaviane , Taylor Jason
Published: October 2016

A series of novel xylitan derivatives derived from xylitol were synthesized using operationally simple procedures. A xylitan acetonide was the key intermediate used to prepare benzoate, arylsulfonate esters and 1,2,3-triazole derivatives of xylitan. These compounds were evaluated for their in vitro anti-Trypanosoma cruzi activity against trypomastigote and amastigote forms of the parasite in T. cruzi-infected cell lineages. Benznidazole was used as positive control against T. cruzi and cytotoxicity was determined in mammalian L929 cells. The arylsulfonate xylitan derivative bearing a nitro group displayed the best activity of all the compounds tested, and was slightly more potent than the reference drug benznidazole. The importance of the isopropylidene ketal moiety was established and the greater lipophilicity of these compounds suggests enhancement in cell penetration.
Modulation of Autophagy by a Thioxanthone Decreases the Viability of Melanoma Cells
Authors: Lima Raquel , Sousa Diana , Paiva Ana , Palmeira Andreia , Barbosa João , Pedro Madalena , Pinto Madalena , Sousa Emília , Vasconcelos M.
Published: October 2016

(1) Background: Our previous studies unveiled the hit thioxanthone TXA1 as an inhibitor of P-glycoprotein (drug efflux pump) and of human tumor cells growth, namely of melanoma cells. Since TXA1 is structurally similar to lucanthone (an autophagy inhibitor and apoptosis inducer) and to N10-substituted phenoxazines (isosteres of thioxanthones, and autophagy inducers), this study aimed at further assessing its cytotoxic mechanism and evaluating its potential as an autophagy modulator in A375-C5 melanoma cells; (2) Methods: Flow cytometry with propidium iodide (PI) for cell cycle profile analysis; Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay, flow cytometry with Annexin V/PI labeling and Western blot for apoptosis analysis were conducted. A pharmacophore approach was used for mapping TXA1 onto pharmacophores for autophagy induction. Autophagy analyses included transmission electron microscopy for visualization of autophagic structures, fluorescence microscopy for observation of monodansylcadaverine (MDC) staining, pattern of LC3 expression in the cells and acridine orange staining, and Western blot for autophagic proteins expression; (3) Results: TXA1 induced autophagy of melanoma cells at the GI50 concentration (3.6 μM) and apoptosis at twice that concentration. Following treatment with TXA1, autophagic structures were observed, together with the accumulation of autophagosomes and the formation of autophagolysosomes. An increase in LC3-II levels was also observed, which was reverted by 3-methyladenine (3-MA) (an early stage autophagy-inhibitor) but further increased by E-64d/pepstatin (late-stage autophagy inhibitors). Finally, 3-MA also reverted the effect of TXA1 in cellular viability; (4) Conclusion: TXA1 decreases the viability of melanoma cells by modulation of autophagy and may, therefore, serve as a lead compound for the development of autophagy modulators with antitumor activity.
Ultraviolet B (UVB) Photosensitivities of Tea Catechins and the Relevant Chemical Conversions
Authors: Shi Meng , Nie Ying , Zheng Xin-Qiang , Lu Jian-Liang , Liang Yue-Rong , Ye Jian-Hui
Published: October 2016

Ultraviolet B (UVB) photosensitivities of eight catechins were screened. In both water and ethanol, epicatechin (EC, 575 μM) and catechin (C, 575 μM) exhibited low photostabilities under 6 h UVB radiation with the generation of yellow photoproducts, while other catechins (epigallocatechin gallate, epigallocatechin, epicatechin gallate, gallocatechingallate, gallocatechin, catechin gallate) were relatively UVB-insensitive. Photoisomerization and photolysis were two important UVB-induced reactions to EC whereas photolysis was the dominant reaction for C. The influencing factors of time (2–10 h), solvent (water, ethanol) and substrate concentration (71.875–1150 μM) on UVB-induced chemical conversions of EC and C were investigated, and eight photoproducts were identified through ultra performance liquid chromatography-diode array detection-tandem mass spectrometry (UPLC-DAD-MS/MS) and 1H nuclear magnetic resonance (1H-NMR analysis). Photolysis reaction involved two pathways, including radical reaction and photo-induced electron transfer reaction. The 2,2-diphenylpicrylhydrazyl (DPPH) scavenging abilities of eight catechins did not change upon 6 h UVB irradiation. EC and C are photosensitive catechins among eight catechins causing deep color.
Probe Microscopic Studies of DNA Molecules on Carbon Nanotubes
Authors: Umemura Kazuo , Izumi Katsuki , Oura Shusuke
Published: October 2016

Hybrids of DNA and carbon nanotubes (CNTs) are promising nanobioconjugates for nanobiosensors, carriers for drug delivery, and other biological applications. In this review, nanoscopic characterization of DNA-CNT hybrids, in particular, characterization by scanning probe microscopy (SPM), is summarized. In many studies, topographical imaging by atomic force microscopy has been performed. However, some researchers have demonstrated advanced SPM operations in order to maximize its unique and valuable functions. Such sophisticated approaches are attractive and will have a significant impact on future studies of DNA-CNT hybrids.
Composition and Nutrient Information of Non-Alcoholic Beverages in the Spanish Market: An Update
Authors: Serrano Iglesias María , De Lourdes Samaniego Vaesken María , Varela Moreiras Gregorio
Published: October 2016

The aim of this study was to draw an updated map of the nutrition facts in the different categories of non-alcoholic beverages in the Spanish market based on the information declared on the labels of these products; we expect this first step to justify the need for the coordination and harmonization of food composition tables in Spain so that there will be an updated database available to produce realistic scientific nutrient intake estimates in accordance with the actual market scenario. Materials and Methods: The nutrition facts declared on the labels of non-alcoholic beverages by manufacturers in Spain were compiled and studied. Results: The database included 211 beverages classified in 7 groups with energy/carbohydrate content per 100 mL ranging from 0–55 kcal/0–13 g for soft drinks; 2–60 kcal/0–14.5 g for energy drinks; 24–31 kcal/5.8–7.5 g for sports drinks; 1–32 kcal/0–7.3 g for drinks containing mineral salts in their composition; 14–69 kcal/2.6–17 g for fruit juice, nectar, and grape musts; 43–78 kcal/6.1–14.4 g for vegetable drinks; and 33–88 kcal/3.6–14 g for dairy drinks. Conclusion: The current non-alcoholic beverage market is a dynamic, growing, and highly innovative one, allowing consumers to choose according to their preferences, needs, or level of physical activity at any moment of the day.
Caffeine Use among Active Duty Navy and Marine Corps Personnel
Authors: Knapik Joseph , Trone Daniel , McGraw Susan , Steelman Ryan , Austin Krista , Lieberman Harris
Published: October 2016

Data from the National Health and Nutrition Examination Survey (NHANES) indicate 89% of Americans regularly consume caffeine, but these data do not include military personnel. This cross-sectional study examined caffeine use in Navy and Marine Corps personnel, including prevalence, amount of daily consumption, and factors associated with use. A random sample of Navy and Marine Corps personnel was contacted and asked to complete a detailed questionnaire describing their use of caffeine-containing substances, in addition to their demographic, military, and lifestyle characteristics. A total of 1708 service members (SMs) completed the questionnaire. Overall, 87% reported using caffeinated beverages ≥1 time/week, with caffeine users consuming a mean ± standard error of 226 ± 5 mg/day (242 ± 7 mg/day for men, 183 ± 8 mg/day for women). The most commonly consumed caffeinated beverages (% users) were coffee (65%), colas (54%), teas (40%), and energy drinks (28%). Multivariable logistic regression modeling indicated that characteristics independently associated with caffeine use (≥1 time/week) included older age, white race/ethnicity, higher alcohol consumption, and participating in less resistance training. Prevalence of caffeine use in these SMs was similar to that reported in civilian investigations, but daily consumption (mg/day) was higher.
Maternal Folate Status and the BHMT c.716G>A Polymorphism Affect the Betaine Dimethylglycine Pathway during Pregnancy
Authors: Colomina Jose , Cavallé-Busquets Pere , Fernàndez-Roig Sílvia , Solé-Navais Pol , Fernandez-Ballart Joan , Ballesteros Mónica , Ueland Per , Meyer Klaus , Murphy Michelle
Published: October 2016

The effect of the betaine: homocysteine methyltransferase BHMT c.716G>A (G: guanosine; A: adenosine) single nucleotide polymorphism (SNP) on the BHMT pathway is unknown during pregnancy. We hypothesised that it impairs betaine to dimethylglycine conversion and that folate status modifies its effect. We studied 612 women from the Reus Tarragona Birth Cohort from ≤12 gestational weeks (GW) throughout pregnancy. The frequency of the variant BHMT c.716A allele was 30.8% (95% confidence interval (CI): 28.3, 33.5). In participants with normal-high plasma folate status (>13.4 nmol/L), least square geometric mean [95% CI] plasma dimethylglycine (pDMG, µmol/L) was lower in the GA (2.35 [2.23, 2.47]) versus GG (2.58 [2.46, 2.70]) genotype at ≤12 GW (p < 0.05) and in the GA (2.08 [1.97, 2.19]) and AA (1.94 [1.75, 2.16]) versus GG (2.29 [2.18, 2.40]) genotypes at 15 GW (p < 0.05). No differences in pDMG between genotypes were observed in participants with possible folate deficiency (≤13.4 nmol/L) (p for interactions at ≤12 GW: 0.023 and 15 GW: 0.038). PDMG was lower in participants with the AA versus GG genotype at 34 GW (2.01 [1.79, 2.25] versus 2.44 [2.16, 2.76] and at labour, 2.51 [2.39, 2.64] versus 3.00 [2.84, 3.18], (p < 0.01)). Possible deficiency compared to normal-high folate status was associated with higher pDMG in multiple linear regression analysis (β coefficients [SEM] ranging from 0.07 [0.04], p < 0.05 to 0.20 [0.04], p < 0.001 in models from early and mid-late pregnancy) and the AA compared to GG genotype was associated with lower pDMG (β coefficients [SEM] ranging from −0.11 [0.06], p = 0.055 to −0.23 [0.06], p < 0.001). Conclusion: During pregnancy, the BHMT pathway is affected by folate status and by the variant BHMT c.716A allele.
Dietary Intake of Trans Fatty Acids in Children Aged 4–5 in Spain: The INMA Cohort Study
Authors: Scholz Alexander , Gimenez-Monzo Daniel , Navarrete-Muñoz Eva , Garcia-de-la-Hera Manuela , Fernandez-Somoano Ana , Tardon Adonina , Santa Marina Loreto , Irazabal Amaia , Romaguera Dora , Guxens Mònica , Julvez Jordi , Llop Sabrina , Lopez-Espinosa Maria-Jose , Vioque Jesus
Published: October 2016

Trans fatty acid (TFA) intake has been identified as a health hazard in adults, but data on preschool children are scarce. We analyzed the data from the Spanish INMA Project to determine the intake of total, industrial and natural TFA, their main sources and the associated socio-demographic and lifestyle factors in children aged 4–5 (n = 1793). TFA intake was estimated using a validated Food Frequency Questionnaire, and multiple linear regression was used to explore associated factors. The mean daily intakes of total, industrial and natural TFA were 1.36, 0.60, and 0.71 g/day, respectively. Ten percent of the children obtained >1% of their energy intake from TFA. The main sources of industrial TFA were fast food, white bread and processed baked goods. Milk, red and processed meat and processed baked goods were the main sources of natural TFA. Having parents from countries other than Spain was significantly associated with higher natural TFA (in mg/day) intake (β 45.5) and television viewing was significantly associated with higher industrial TFA intake (β 18.3). Higher fruits and vegetables intake was significantly associated with lower intakes of all TFAs, whereas higher sweetened beverages intake was significantly associated with lower total and natural TFA intake. Thus, total and industrial TFA intake was associated with less healthy food patterns and lifestyles in Spanish preschool children.
Cross-Linked Dependency of Boronic Acid-Conjugated Chitosan Nanoparticles by Diols for Sustained Insulin Release
Authors: Siddiqui Nabil , Billa Nashiru , Roberts Clive , Asantewaa Osei Yaa
Published: October 2016

Boronic acids have been widely investigated for their potential use as glucose sensors in glucose responsive polymeric insulin delivery systems. Interactions between cyclic diols and boronic acids, anchored to polymeric delivery systems, may result in swelling of the delivery system, releasing the drug. In this study, 4-formylphenylboronic acid conjugated chitosan was formulated into insulin containing nanoparticles via polyelectrolyte complexation. The nanoparticles had an average diameter of 140 ± 12.8 nm, polydispersity index of 0.17 ± 0.1, zeta potential of +19.1 ± 0.69 mV, encapsulation efficiency of 81% ± 1.2%, and an insulin loading capacity of 46% ± 1.8% w/w. Changes in size of the nanoparticles and release of insulin were type of sugar- and concentration-dependent. High concentration of diols resulted in a sustained release of insulin due to crosslink formation with boronic acid moieties within the nanoparticles. The formulation has potential to be developed into a self-regulated insulin delivery system for the treatment of diabetes.
Understanding Plant Nitrogen Metabolism through Metabolomics and Computational Approaches
Authors: Beatty Perrin , Klein Matthias , Fischer Jeffrey , Lewis Ian , Muench Douglas , Good Allen
Published: October 2016

A comprehensive understanding of plant metabolism could provide a direct mechanism for improving nitrogen use efficiency (NUE) in crops. One of the major barriers to achieving this outcome is our poor understanding of the complex metabolic networks, physiological factors, and signaling mechanisms that affect NUE in agricultural settings. However, an exciting collection of computational and experimental approaches has begun to elucidate whole-plant nitrogen usage and provides an avenue for connecting nitrogen-related phenotypes to genes. Herein, we describe how metabolomics, computational models of metabolism, and flux balance analysis have been harnessed to advance our understanding of plant nitrogen metabolism. We introduce a model describing the complex flow of nitrogen through crops in a real-world agricultural setting and describe how experimental metabolomics data, such as isotope labeling rates and analyses of nutrient uptake, can be used to refine these models. In summary, the metabolomics/computational approach offers an exciting mechanism for understanding NUE that may ultimately lead to more effective crop management and engineered plants with higher yields.
Mapping Winter Wheat Biomass and Yield Using Time Series Data Blended from PROBA-V 100- and 300-m S1 Products
Authors: Zheng Yang , Zhang Miao , Zhang Xin , Zeng Hongwei , Wu Bingfang
Published: October 2016

Monitoring crop areas and yields is crucial for food security and agriculture management across the world. In this paper, we mapped the biomass and yield of winter wheat using the new Project for On-Board Autonomy-Vegetation (PROBA-V) products in the North China Plain (NCP). First, the daily 100-m land surface reflectance was generated by fusing the PROBA-V 100-m and 300-m S1 products. Our results show that the blended data exhibited high correlations with the referenced data (0.71 ≤ R2 ≤ 0.94 for the red band, 0.50 ≤ R2 ≤ 0.95 for the near-infrared band, and 0.88 ≤ R2 ≤ 0.97 for the shortwave infrared band). The time-series Normalized Difference Vegetation Index (NDVI) derived from the synthetic reflectance was then clustered for winter wheat identification. The overall classification accuracy was between 78% and 87%, with a kappa coefficient above 0.57, which was 10%–20% higher than the classification accuracy using the 300-m data. Finally, a light use efficiency model was employed to estimate the biomass and yield. The estimation results were closely related to the field-measured biomass and yield, with high R2 and low root mean square errors (RMSE) (0.864 ≤ R2 ≤ 0.871 and 168 ≤ RMSE ≤ 191 g/m2 for biomass; and 0.631 ≤ R2 ≤ 0.663 and 41.8 ≤ RMSE ≤ 62.8 g/m2 for yield). This paper shows the strong potential of using PROBA-V 100-m data to enhance the spatial resolution of PROBA-V 300-m data and because the proposed framework in this study was based only on the relatively high spatio-temporal resolution PROBA-V data and achieved favorable results, it provides a novel approach for crop areas and yields estimation utilizing the relatively new data set.
A New Image Processing Procedure Integrating PCI-RPC and ArcGIS-Spline Tools to Improve the Orthorectification Accuracy of High-Resolution Satellite Imagery
Authors: Zhang Hongying , Pu Ruiliang , Liu Xiuguo
Published: October 2016

Given the low accuracy of the traditional remote sensing image processing software when orthorectifying satellite images that cover mountainous areas, and in order to make a full use of mutually compatible and complementary characteristics of the remote sensing image processing software PCI-RPC (Rational Polynomial Coefficients) and ArcGIS-Spline, this study puts forward a new operational and effective image processing procedure to improve the accuracy of image orthorectification. The new procedure first processes raw image data into an orthorectified image using PCI with RPC model (PCI-RPC), and then the orthorectified image is further processed using ArcGIS with the Spline tool (ArcGIS-Spline). We used the high-resolution CBERS-02C satellite images (HR1 and HR2 scenes with a pixel size of 2 m) acquired from Yangyuan County in Hebei Province of China to test the procedure. In this study, when separately using PCI-RPC and ArcGIS-Spline tools directly to process the HR1/HR2 raw images, the orthorectification accuracies (root mean square errors, RMSEs) for HR1/HR2 images were 2.94 m/2.81 m and 4.65 m/4.41 m, respectively. However, when using our newly proposed procedure, the corresponding RMSEs could be reduced to 1.10 m/1.07 m. The experimental results demonstrated that the new image processing procedure which integrates PCI-RPC and ArcGIS-Spline tools could significantly improve image orthorectification accuracy. Therefore, in terms of practice, the new procedure has the potential to use existing software products to easily improve image orthorectification accuracy.
A Sparsity-Based InSAR Phase Denoising Algorithm Using Nonlocal Wavelet Shrinkage
Authors: Fang Dongsheng , Lv Xiaolei , Wang Yong , Lin Xue , Qian Jiang
Published: October 2016

An interferometric synthetic aperture radar (InSAR) phase denoising algorithm using the local sparsity of wavelet coefficients and nonlocal similarity of grouped blocks was developed. From the Bayesian perspective, the double- l 1 norm regularization model that enforces the local and nonlocal sparsity constraints was used. Taking advantages of coefficients of the nonlocal similarity between group blocks for the wavelet shrinkage, the proposed algorithm effectively filtered the phase noise. Applying the method to simulated and acquired InSAR data, we obtained satisfactory results. In comparison, the algorithm outperformed several widely-used InSAR phase denoising approaches in terms of the number of residues, root-mean-square errors and other edge preservation indexes.
Urban Heat Islands as Viewed by Microwave Radiometers and Thermal Time Indices
Authors: Nguyen Lan , Henebry Geoffrey
Published: October 2016

Urban heat islands (UHIs) have been long studied using both ground-based observations of air temperature and remotely sensed thermal infrared (TIR) data. While ground-based observations lack spatial detail even in the occasional “dense” urban network, skin temperature retrievals using TIR data have lower temporal coverage due to revisit frequency, limited swath width, and cloud cover. Algorithms have recently been developed to retrieve near-surface air temperatures using microwave radiometer data, which enables characterization of UHIs in metropolitan areas, major conurbations, and global megacities at regional to continental scales using temporally denser time series than those that have been available from TIR sensors. Here we examine how UHIs appear across the entire Western Hemisphere using surface air temperatures derived from the Advanced Microwave Scanning Radiometers (AMSRs), AMSR-E onboard the National Aeronautics and Space Administration’s (NASA’s) Aqua and AMSR2 onboard the Japan Aerospace eXploration Agency’s Global Change Observation Mission-Water1 (JAXA’s GCOM-W1) satellites. We compare these data with station observations from the Global Historical Climate Network (GHCN) for 27 major cities across North America (in 83 urban-rural groupings) to demonstrate the capability of microwave data in a UHI study. Two measures of thermal time, accumulated diurnal and nocturnal degree-days, are calculated from the remotely sensed surface air temperature time series to characterize the urban-rural thermal differences over multiple growing seasons. Daytime urban thermal accumulations from the microwave data were sometimes lower than in adjacent rural areas. In contrast, station observations showed consistently higher day and night thermal accumulations in cities. UHIs are more pronounced at night, with 55% (AMSRs) and 93% (GHCN) of urban-rural groupings showing higher accumulated nocturnal degree-days in cities. While urban-rural thermal gradients may vary according to different datasets or locations, day-night differences in thermal time metrics were consistently lower (>90% of urban-rural groupings) in urban areas than in rural areas for both datasets. We propose that the normalized difference accumulated thermal time index (NDATTI) is a more robust metric for comparative UHI studies than simple temperature differences because it can be calculated from either station or remotely sensed data and it attenuates latitudinal effects.
Graphene Nanogrids FET Immunosensor: Signal to Noise Ratio Enhancement
Authors: Basu Jayeeta , RoyChaudhuri Chirasree
Published: October 2016

Recently, a reproducible and scalable chemical method for fabrication of smooth graphene nanogrids has been reported which addresses the challenges of graphene nanoribbons (GNR). These nanogrids have been found to be capable of attomolar detection of biomolecules in field effect transistor (FET) mode. However, for detection of sub-femtomolar concentrations of target molecule in complex mixtures with reasonable accuracy, it is not sufficient to only explore the steady state sensitivities, but is also necessary to investigate the flicker noise which dominates at frequencies below 100 kHz. This low frequency noise is dependent on the exposure time of the graphene layer in the buffer solution and concentration of charged impurities at the surface. In this paper, the functionalization strategy of graphene nanogrids has been optimized with respect to concentration and incubation time of the cross linker for an enhancement in signal to noise ratio (SNR). It has been interestingly observed that as the sensitivity and noise power change at different rates with the functionalization parameters, SNR does not vary monotonically but is maximum corresponding to a particular parameter. The optimized parameter has improved the SNR by 50% which has enabled a detection of 0.05 fM Hep-B virus molecules with a sensitivity of around 30% and a standard deviation within 3%. Further, the SNR enhancement has resulted in improvement of quantification accuracy by five times and selectivity by two orders of magnitude.
An Enhanced Lightweight Anonymous Authentication Scheme for a Scalable Localization Roaming Service in Wireless Sensor Networks
Authors: Chung Youngseok , Choi Seokjin , Lee Youngsook , Park Namje , Won Dongho
Published: October 2016

More security concerns and complicated requirements arise in wireless sensor networks than in wired networks, due to the vulnerability caused by their openness. To address this vulnerability, anonymous authentication is an essential security mechanism for preserving privacy and providing security. Over recent years, various anonymous authentication schemes have been proposed. Most of them reveal both strengths and weaknesses in terms of security and efficiency. Recently, Farash et al. proposed a lightweight anonymous authentication scheme in ubiquitous networks, which remedies the security faults of previous schemes. However, their scheme still suffers from certain weaknesses. In this paper, we prove that Farash et al.’s scheme fails to provide anonymity, authentication, or password replacement. In addition, we propose an enhanced scheme that provides efficiency, as well as anonymity and security. Considering the limited capability of sensor nodes, we utilize only low-cost functions, such as one-way hash functions and bit-wise exclusive-OR operations. The security and lightness of the proposed scheme mean that it can be applied to roaming service in localized domains of wireless sensor networks, to provide anonymous authentication of sensor nodes.