bluuebunny/crossref_metadata_2025 huggingface dataset:
Sex-specific responses to slow progressive pressure overload in a large animal model of HFpEF
Authors: Eaton Deborah M. , Berretta Remus M. , Lynch Jacqueline E. , Travers Joshua G. , Pfeiffer Ryan D. , Hulke Michelle L. , Zhao Huaqing , Hobby Alexander R. H. , Schena Giana , Johnson Jaslyn P. , Wallner Markus , Lau Edward , Lam Maggie P. Y. , Woulfe Kathleen C. , Tucker Nathan R. , McKinsey Timothy A. , Wolfson Marla R. , Houser Steven R.
Published: September 2022

We performed a comprehensive assessment to evaluate the effects of slow progressive pressure overload on cardiopulmonary function in a large animal model of heart failure with preserved ejection fraction (HFpEF) in males and females. Functional and structural assessments were performed at the organ, tissue, cellular, protein, and transcriptional levels. This is the first study to compare snRNAseq and ECM mass spectrometry of HFpEF myocardium from males and females. The results broaden our understanding of the pathophysiological response of both sexes to pressure overload. Both sexes developed a robust cardiopulmonary phenotype, but the phenotype was equal or a bit less robust in females.
Dog and human bladders have different neurogenic and nicotinic responses in inner versus outer detrusor muscle layers
Authors: Frara Nagat , Barbe Mary F. , Giaddui Dania , Braverman Alan S. , Amin Mamta , Yu Daohai , Ruggieri Michael R.
Published: September 2022

The aim of this study was to investigate layer and species variations in detrusor muscle strip responses to myogenic, neurogenic, and nicotinic, and muscarinic receptor stimulations. Strips from bladders of 9 dogs and 6 human organ transplant donors were dissected from inner and outer longitudinal muscle layers, at least 1 cm above urethral orifices. Strips were mounted in muscle baths and maximal responses to neurogenic stimulation using electrical field stimulation (EFS) and myogenic stimulation using potassium chloride (KCl, 120 mM) determined. After washing and re-equilibration was completed, responses to nicotinic receptor agonist epibatidine (10 μM) were determined followed by responses to EFS and muscarinic receptor agonist bethanechol (30 μM) in continued presence of epibatidine. Thereafter, strips and full-thickness bladder sections from four additional dogs and three human donors were examined for axonal density and intramural ganglia. In dog bladders, contractions to KCl, epibatidine, and bethanechol were 1.5- to 2-fold higher in the inner longitudinal muscle layer, whereas contractions to EFS were 1.5-fold higher in the outer (both pre- and post-epibatidine). Human bladders showed 1.2-fold greater contractions to epibatidine in the inner layer and to EFS in the outer, yet no layer differences to KCl or bethanechol were noted. In both species, axonal density was 2- to 2.5-fold greater in the outer layer. Dogs had more intramural ganglia in the adventitia/serosa layer, compared with more internal layers and to humans. These findings indicate several layer-dependent differences in receptor expression or distribution, and neurogenic responses in dog and human detrusor muscles, and myogenic/muscarinic differences between dog versus humans.
Integrated genomics approaches identify transcriptional mediators and epigenetic responses to Afghan desert particulate matter in small airway epithelial cells
Authors: Gupta Arnav , Sasse Sarah K. , Berman Reena , Gruca Margaret A. , Dowell Robin D. , Chu Hong Wei , Downey Gregory P. , Gerber Anthony N.
Published: September 2022

Military Deployment to Southwest Asia and Afghanistan and exposure to toxic airborne particulates have been associated with an increased risk of developing respiratory disease, collectively termed deployment-related respiratory diseases (DRRDs). Our knowledge about how particulates mediate respiratory disease is limited, precluding the appropriate recognition or management. Central to this limitation is the lack of understanding of how exposures translate into dysregulated cell identity with dysregulated transcriptional programs. The small airway epithelium is involved in both the pathobiology of DRRD and fine particulate matter deposition. To characterize small airway epithelial cell epigenetic and transcriptional responses to Afghan desert particulate matter (APM) and investigate the functional interactions of transcription factors that mediate these responses, we applied two genomics assays, the assay for transposase accessible chromatin with sequencing (ATAC-seq) and Precision Run-on sequencing (PRO-seq). We identified activity changes in a series of transcriptional pathways as candidate regulators of susceptibility to subsequent insults, including signal-dependent pathways, such as loss of cytochrome P450 or P53/P63, and lineage-determining transcription factors, such as GRHL2 loss or TEAD3 activation. We further demonstrated that TEAD3 activation was unique to APM exposure despite similar inflammatory responses when compared with wood smoke particle exposure and that P53/P63 program loss was uniquely positioned at the intersection of signal-dependent and lineage-determining transcriptional programs. Our results establish the utility of an integrated genomics approach in characterizing responses to exposures and identifying genomic targets for the advanced investigation of the pathogenesis of DRRD.
Global phosphoproteomic profiling of skeletal muscle in ovarian hormone-deficient mice
Authors: Peyton Mina P. , Yang Tzu-Yi , Higgins LeeAnn , Markowski Todd W. , Vue Cha , Parker Laurie L. , Lowe Dawn A.
Published: September 2022

Protein phosphorylation is important in skeletal muscle development, growth, regeneration, and contractile function. Alterations in the skeletal muscle phosphoproteome due to aging have been reported in males; however, studies in females are lacking. We have demonstrated that estrogen deficiency decreases muscle force, which correlates with decreased myosin regulatory light chain phosphorylation. Thus, we questioned whether the decline of estrogen in females that occurs with aging might alter the skeletal muscle phosphoproteome. C57BL/6J female mice (6 mo) were randomly assigned to a sham-operated (Sham) or ovariectomy (Ovx) group to investigate the effects of estrogen deficiency on skeletal muscle protein phosphorylation in a resting, noncontracting condition. After 16 wk of estrogen deficiency, the tibialis anterior muscle was dissected and prepped for label-free nano-liquid chromatography-tandem mass spectrometry phosphoproteomic analysis. We identified 4,780 phosphopeptides in tibialis anterior muscles of ovariectomized (Ovx) and Sham-operated (Sham) control mice. Further analysis revealed 647 differentially regulated phosphopeptides (Benjamini–Hochberg adjusted P value < 0.05 and 1.5-fold change ratio) that corresponded to 130 proteins with 22 proteins differentially phosphorylated (3 unique to Ovx, 2 unique to Sham, 6 upregulated, and 11 downregulated). Differentially phosphorylated proteins associated with the sarcomere, cytoplasm, and metabolic and calcium signaling pathways were identified. Our work provides the first global phosphoproteomic analysis in females and how estrogen deficiency impacts the skeletal muscle phosphoproteome.
Evaluation and Analysis of Land Input-Output Comprehensive Benefit Based on Fuzzy Mathematics and Analytic Hierarchy Process
Authors: Zhu Xincheng , Zhang Yan , Hou Yunzhi , Jiang Minda
Published: September 2022

Land use and comprehensive land evaluation are essential. Based on fuzzy mathematics theory and biological heuristic algorithm, the land input-output benefits are evaluated comprehensively. This paper firstly selects six indicators from six aspects of land resource input, capital input, and economic output, and so on. Based on land input and output, this paper constructs the evaluation indicator system of comprehensive benefit for land use. Then, based on the theory of fuzzy mathematics, the improved particle swarm optimization (PSO) algorithm is used to identify the fuzzy density value of the evaluation index. Combined with the analytic hierarchy process (AHP), comprehensive evaluation method, through the combination of subjective and objective methods, is comprehensively evaluating land benefits. Finally, the Tobit model is constructed to further analyze the influencing factors of total factor productivity of urban land use and explore the influencing mechanism of government regulation, land opening, and other factors and land development. The research results of this paper can provide reference for future urban planning, land structure adjustment, land resource utilization and protection, food security, ecological security, economic security, and so on.
Influences of Antithrombotic Elastic Socks Combined with Air Pressure in Reducing Lower Extremity Deep Venous Thrombosis for Patients Undergoing Cardiothoracic Surgery
Authors: Fu Weihong , Zhang Qun , Sun Xuemei , Gu Yan
Published: September 2022

This study was designed to investigate the application and therapeutic effect of antithrombotic elastic socks combined with air pressure in the prevention of lower extremity deep venous thrombosis in patients undergoing cardiothoracic surgery. Sixty patients in cardiothoracic surgery of our hospital from January 2019 to December 2020 were randomly divided into a study group and control group. The control group was treated with routine treatment intervention. Based on routine treatment intervention, the study group was treated with antithrombotic elastic socks combined with pneumatic treatment intervention. The activated partial thromboplastin time (APTT), thrombin time (TT), femoral venous blood flow velocity of both lower limbs, and the incidence of lower extremity deep venous thrombosis (LEDVT), postoperative lower extremity swelling, inflammatory factors, and satisfaction were measured. After intervention, APTT (

31.74
±
1.15

 s) and TT (

14.58
±
0.24

 s) in the study group were higher than those in the control group APTT (

25.13
±
1.14

 s) and TT (

12.14
±
0.23

 s) (

P
<
0.05

). The left lower limb femoral vein blood flow velocity and the right lower limb femoral vein blood flow velocity in the study group were better than those in the control group (

P
<
0.05

). The incidence of postoperative lower limb swelling and deep vein in the study group was lower than that in the control group (

P
<
0.05

). Serum tumor necrosis factor alpha and interleukin-6 concentrations in the study group were lower than those in the control group (

P
<
0.05

). The satisfaction rate of patients in the study group (93.33%) was significantly higher than that in the control group (70.00%) (

P
<
0.05

). In conclusion, after cardiothoracic surgery, antithrombotic elastic socks combined with air pressure can significantly reduce the incidence of LEDVT by improving patients’ coagulation function, reducing inflammatory reaction. It is worthy of popularization and application in relevant surgery.
The Basic Principles of Marxism with the Internet as a Carrier
Authors: Ruichen Wang
Published: September 2022

We live in a real society today with the Internet as the carrier. Marxist philosophy is of great significance to the study of computer science and technology majors, such as the standardization of learning methods and the improvement of learning horizons. In order to study the ideology of the current Internet carrier, this study first introduces the ideology in the Internet scenario and again describes the basic principles of Marxism. Web text data were collected from both static and dynamic websites, and the data were initially preprocessed using the ICTCLAS word separation tool. After that, a text lexicon set related to Marxism was built and combined with the surplus value calculation formula. Analysis of the text data shows that the expectation error of the Internet population approximately obeys a positive-term distribution, and the online text data are feasible in the estimation of the influence of Marxism. The statistical results show that the percentage of serious philosophical discussions from 2017 to 2021 in which Marxism is mentioned has steadily increased from 62% to 78%, indicating that Marxism still has great influence to this day. For these three different attitudes toward Marxism, the number of comments agreeing and disagreeing differed significantly, indicating that different groups of people show very different attitudes toward Marxism.
Fatigue and Sleep Disturbance among Breast Cancer Patients during Treatment in Saudi Arabia
Authors: Alsharif Fatmah , Shibily Faygah , Almutairi Wedad , Alsaedi Ahlam , Alsubaie Tahani , Alshuaibi Bashayer , Turkistani Arwa
Published: September 2022

Background. Fatigue and sleep disturbances are highly prevalent side effects encountered during treatment by patients with breast cancer, and they affect women’s quality of life. Most studies investigating sleep and exhaustion in cancer patients provide evidence that supports a strong connection between different sleep parameters and fatigue associated with cancer. Objective. This study was to assess the level of fatigue and sleep disturbance in breast cancer patients during treatment in Saudi Arabia. Method. A descriptive cross-sectional study design was conducted on breast cancer patients undergoing treatment in Saudi Arabia. The data were collected through convenience sampling of patients. The study involved self-administered questionnaires comprising three categories: demographic data, perceived Pittsburgh Sleep Quality Index (PSQI), and Functional Assessment of Chronic Illness Therapy–Fatigue (FACIT-F). A total of 101 participants took part in this study. The significant statistical test was determined at a 95% confidence interval and at

p
<
0.05

. Results. Half of the study participants were aged between 30 and 50 years. Significant differences were found in fatigue and sleep disturbance among BC patients during treatment in Saudi Arabia. A high prevalence of fatigue was found at 21.8%, and 5% of participants experienced sleep disturbance. Conclusions. Breast cancer patients in Saudi Arabia have a low overall global quality of life. The patients experience poor sleep quality and fatigue, which suggests that during treatment, patients need to be assessed routinely for these symptoms to prevent or reduce fatigue and sleep disturbance. Sleep disturbance might be correlated with fatigue.
CKAP2L Promotes Esophageal Squamous Cell Carcinoma Progression and Drug-Resistance by Modulating Cell Cycle
Authors: Chen Wenhu , Wang Yu , Wang Lifang , Zhao Hongguang , Li Xiaoyan
Published: September 2022

Esophageal squamous cell carcinoma (ESCC) is one of the most common types of cancer and the leading cause of cancer-related mortality worldwide, especially in Asia. In this study, the gene CKAP2L was selected by GEO, TCGA, and GTEx database analysis. The high expression of CKAP2L is related to the occurrence and development of ESCC. In addition, CKAP2L knockdown can inhibit the growth and migration of ESCC cells, while CKAP2L overexpression has the opposite effect. Furthermore, in vivo experiments indicated that down-regulation of CKAP2L can inhibit the tumorigenesis of ESCC cells. KEGG pathway analysis and the STRING database explored the relationship between cell cycle and CKAP2L and verified that depletion of CKAP2L markedly arrested cell cycle in the G2/M phase. Meanwhile, CKAP2L knockdown increased the sensitivity of ESCC cells to flavopiridol, the first CDK inhibitor to be tested in clinical trials, leading to an observable reduction in cell proliferation and an increase in cellular apoptosis. In brief, we identified CKAP2L as a tumor promoter, potential prognostic indicator, and therapeutic target of ESCC, which may play a role in regulating cell cycle progression.
[Retracted] Radial Neural Network Processing Applied to Athlete’s Personalized Psychological Regulation Detection System
Authors: Xu Weihua , Li Qiang , Wang Yi
Published: September 2022

Radial neural network is based in the world with the characteristics of active adaptation, active learning, active recognition, low error rate, and thought mapping and plays an important role in personalized regulation. However, in practical applications, due to the existence of pattern recognition, motion control, and a large amount of combined knowledge, the traditional methods are difficult to solve, even powerless, and cannot be effectively solved. Although the traditional BP network is more widely used, the BP neural network is easy to enter the regional minimum value during the training process, which leads to a lower training learning speed and low efficiency. The RBF network (radial neural network) is in a certain sense, and it can detect both known intrusions and unpredictable intrusions. At the same time, it is superior to BP neural network in data collection, pattern recognition, and personality customization. Through detection and comparison, it is found that the radial network has improved the analysis speed by about 20%, and the degree of privacy protection of athletes is as high as 99%, which is close to the full value, and the accuracy of the psychological control scheme is also improved by about 15% on the basis of the classic network. Athletes can be adjusted as soon as possible to achieve the best state; so, it will be an ideal choice to use a radial neural network for the psychological adjustment detection system.
Downregulated miR-150-5p in the Tissue of Nasopharyngeal Carcinoma
Authors: Wen Jia-Ying , Chen Gang , Li Jian-Di , Luo Jia-Yuan , He Juan , Wang Ren-Sheng , Qin Li-Ting
Published: September 2022

The clinical significance and potential targets of miR-150-5p have not been elucidated in nasopharyngeal carcinoma (NPC). The pooled analysis based on 539 NPC samples and 75 non-NPC nasopharyngeal samples demonstrated that the expression of miR-150-5p was down-regulated in NPC, with the area under the curve being 0.89 and the standardized mean difference being −0.66. Subsequently, we further screened the differentially expressed genes (DEGs) of 14 datasets, including 312 NPC samples and 70 non-NPC nasopharyngeal samples. After the DEGs were narrowed down with the predicted targets from the miRWalk database, 1316 prospective target genes of miR-150-5p were identified. The enrichment analysis suggested that “pathways in cancer” was the most significant pathway. Finally, six hub genes of “pathways in cancer”, including EGFR, TP53, HRAS, CCND1, CDH1, and FGF2, were screened out through the STRING database. In conclusion, the down-regulation of miR-150-5p modulates the tumorigenesis and progression of NPC.
[Retracted] Influence of Family Sports Games on the Development of Early Communication Skills in Autistic Children
Authors: Hu Jinxiu , Zhou Wenli , Fu Zhonglian , Zeng Xiaomei , Huang Cunquan
Published: September 2022

With the development of society, the number of autistic children in China is increasing, which not only makes the family’s happiness very low, but also seriously affects the development of teenagers and society. Among the symptoms of autistic children, early childhood communication skills have received extensive attention. In traditional rehabilitation training, with a lack of parents’ participation, most of the training cannot arouse the interest of autistic children, so the treatment effect is not obvious. Based on this, this paper proposes the application of family sports games to improve the early communication ability of autistic children. This article aims to investigate the role of family sports games in promoting the development of early communication skills in autistic children. This paper uses the fuzzy comprehensive evaluation method to score the comprehensive ability of family sports games. The experimental results of this paper show that before the experiment, the comprehensive scores of children’s communication ability in the control group and the experimental group were 18.92 and 18, respectively, which were generally low, and there was no significant difference. This shows that the communication skills of the two groups of children before the experiment are relatively poor. After the test, the children’s comprehensive score of communication ability in the experimental group increased by 35.8 points, and the difference was significant, indicating that family sports games have a great impact on the development of children’s communication ability.
Physical and Mechanical Characterization of Bamboo Fiber/Groundnut Shell/Copper Particle/MWCNT‐Filled Epoxy Hybrid Polymer Nanocomposites
Authors: Ramu S. , Senthilkumar N. , Rajendran Saravanan , Deepanraj B. , Abdeta Dereje Bayisa
Published: September 2022

Current research finds the suitability of natural fiber‐reinforced polymer composites for industrial, automotive, marine, aerospace, and military vehicle applications, among others; they process being lightweight, high strength, ecofriendly, recyclable, and biodegradable. Bamboo fiber (Bambusoideae) mat, groundnut shell (Cocos nucifera) powder, copper particle, and multiwalled carbon nanotubes (MWCNT) fillers with epoxy resin are the primary materials. The key responsibility of MWCNT as a nanofiller is to improve the performance of materials by more than 80% and to attract the desirable properties such as high specific energy absorption of CNTs. An extensive investigation was carried out to determine the weight ratio between the total density and the water absorption rate of the composite. The maximal tensile and flexural strengths derived from EBGCM2 (E: epoxy; B: bamboo; G: groundnut shell; C: copper particle; M: MWCNT) were 50.66 MPa and 59.03 MPa, respectively. Addition of MWCNTs to specimens EBGCM2 and EBGCM3 and bonding the filler matrix by homogeneous mixing, but few nanoparticles are seen agglomerated. Flexural abilities of groundnut fiber/filler loading to strengthen the hybrid nanocomposites and the impact strength both quite improved than EB1 specimen. The neat epoxy and bamboo fiber composite (EB1) achieved 87.5 Shore‐D hardness. SEM revealed (EBGCM3) improved interfacial adhesion between fiber and epoxy resin, but few nanoparticles are agglomerated, small molecules linked between the fibrous matrix, and the binding elements trapped in neighboring atoms, forming structural chains by the polymerization process.
Neural Network Based Adaptive Backstepping Control for Electro-Hydraulic Servo System Position Tracking
Authors: Wan Zhenshuai , Yue Longwang , Fu Yu
Published: September 2022

The modeling uncertainties and external disturbances of electro-hydraulic servo system (EHSS) deteriorate the system’s trajectory tracking performance. To cope with this issue, an adaptive backstepping controller based on neural network (NN) is proposed in this paper. A radial-basis-function neural network (RBF NN) is constructed to approximate the lumped uncertainties caused by modeling uncertainties and external disturbances, where the adaptive law is adopted to adjust controller parameters online. The backstepping control is used to eliminate mismatched nonlinear terms and stabilize the system. The dynamic surface control (DSC) is adopted to handle the “explosion of complexity” problem of backstepping method and reduce the computational burden. Compared to the traditional backstepping control, the proposed control scheme improves the steady-state tracking precision and makes the control signal smaller. In addition, the stability analysis shows that the tracking error can asymptotically converge to zero in the face of time-varying unknown dynamics. Simulation and experiment results demonstrate the effectiveness of the controller in term of tracking accuracy and disturbance rejection in comparison with other controllers for the EHSS.
Voltage Frequency Ratio Control Method of the Cycloconverter Based on the GSO Algorithm
Authors: Xu Zhengwang , Yu Jiaqi
Published: September 2022

In order to keep the flux constant when the asynchronous motor is running under load, especially under light load, a Glowworm swarm optimization (GSO) algorithm is proposed to control the voltage frequency ratio of the cycloconverter. Based on the analysis of the two variable control theory of the voltage frequency ratio of the cycloconverter and the working principle of the cycloconverter, the self-control method of the voltage frequency ratio of the cycloconverter by adaptive fuzzy backstepping sliding mode is proposed. In the control process, GSO and BFA are integrated to optimize the control process of the controller, and an adaptive selection strategy is adopted to ensure the adaptive step size of the firefly and improve the accuracy of the GSO algorithm. The experimental results show that when the optimal voltage-frequency ratio is switched between the online continuous frequency control strategy and the frequency range is small enough, the motor speed is stable, the speed regulation process is stable, and the motor is always in the state of high force index. It shows that the control method has a fast convergence speed and a good control effect on the voltage frequency ratio of the ring converter of the experimental motor, and it can keep the flux constant under light load.
Computer Dynamic Simulation Design of Ecological Packaging of New Energy Products Based on Internet of Things Technology
Authors: Zhang Jinna
Published: September 2022

The application of Internet of Things (IoT) technology has realized the transformation of people’s production and lifestyle, and also promoted the transformation of education and teaching mode. At present, the application of informatization in the field of product packaging is increasing. Advanced methods and techniques for visual presentation using virtual reality technology. This study has aimed to realize the process optimization of new energy product packaging design in the context of the Internet of Things to promote the ecological development of the product packaging industry. This study has proposed to use the IoT technology and virtual reality technology to know the integration of new energy product packaging and virtual simulation experiments, which is helpful to analyze and solve the objective system imbalance and ecological imbalance in the product packaging system. Drawing on the principles and laws of the IoT and virtual reality technology, the dynamic simulation process of ecological packaging is constructed and optimized to realize the “ecologicalization” of the packaging field. In a study of RFID tag algorithms in the context of IoT, the least data is transmitted at m = 4, which saves an average of 48 bits per identified tag compared to m = 6. Due to the number of queries and the amount of data transferred, the MICT algorithm chooses m = 4 when identifying 96-bit tags. In terms of recognition efficiency, the MICT algorithm achieves about 74%. Therefore, the dynamic simulation design of product packaging is very important at present.
Body Fat Evaluation in Male Athletes from Combat Sports by Comparing Anthropometric, Bioimpedance, and Dual‐Energy X‐Ray Absorptiometry Measurements
Authors: Dimitrijevic Marko , Paunovic Verica , Zivkovic Vladimir , Bolevich Sergey , Jakovljevic Vladimir
Published: September 2022

Multiple anthropometric equations have been developed aiming to provide accurate and affordable assessment of body fat composition in male athletes. This study examined correlations of values obtained from seventeen different anthropometric equations to DXA as well as BIA and DXA values. Male athletes (n = 101) from three different combat sports, wrestling (n = 33), judo (n = 35), and kickboxing (n = 33), with an average age of 20.9 ± 4.2 were included. Body fat percentage was estimated using anthropometry, BIA, and DXA. Correlations between anthropometric methods and DXA, as well as BIA and DXA, were determined using Spearman’s rank correlation. Sixteen out of seventeen estimates of body fat percentages using existing anthropometric equations showed strong positive correlation with the values derived from DXA measurements (r = 0.569 − 0.909). The highest correlation was observed using the equation derived by Yuhasz, r = 0.909, followed by the equations from Oliver et al., Evans et al., Faulkner, and Thorland et al. (r ≈ 0.9). Statistical analysis of body fat percentages from DXA and BIA measurements also showed high positive correlation (r = 0.710). Correlation of seventeen anthropometric equations with BIA and DXA methods revealed that equations by Yuhasz, Oliver et al., Evans et al., Faulkner, and Thorland et al. are suitable alternative for assessing body fat percentage among male athletes from combat sports, showing even stronger correlation than BIA method.
Identification of Warning Transition Points from Hepatitis B to Hepatocellular Carcinoma Based on Mutation Accumulation for the Early Diagnosis and Potential Drug Treatment of HBV-HCC
Authors: Xu Fei , Meng Qingkang , Wu Feng , Wang Yakun , Yang Wenjun , Tong Yun , Liu Lei , Chen Xiujie
Published: September 2022

The accumulation of multiple genetic mutations is essential during the occurrence and development of hepatocellular carcinoma induced by hepatitis B (HBV-HCC), but understanding their cooperative effects and identifying the warning transition point from hepatitis B to HCC are challenges. In the genomic analysis of somatic mutations of the patient with HBV-HCC in a patient-specific protein-protein interaction (ps-PPI) network, we find mutation influence can propagate along the ps-PPI network. Therefore, in the article, we got the mutation cluster as a new research unit using the Random Walks with Restarts algorithm that is used to describe the efficient boundary of mutation influences. The connection of mutation cluster leads to dysregulation of signaling pathways corresponding to HCC, while dysregulated signaling pathways accumulate gradually and experience a process from quantitative to qualitative changes including a critical mutation cluster called transition point (TP) from hepatitis B to HCC. Moreover, two subtypes of HCC patients with different prognosis and their corresponding biological and clinical characteristics were identified according to TP. The poor prognosis HCC subtype was associated with significant metabolic pathway dysregulation and lower immune cell infiltration, while we also identified several preventive drugs to block the transformation of hepatitis B to hepatocellular carcinoma. The network-level study integrated multiomics data not only showed the sequence of multiple somatic mutations and their cooperative effect but also identified the warning transition point in HCC tumorigenesis for each patient. Our study provides new insight into exploring the cooperative molecular mechanism of chronic inflammatory malignancy in the liver and lays the foundation for the development of new approaches for early prediction and diagnosis of hepatocellular carcinoma and personalized targeted therapy.
The Anti-Inflammatory Effect of Preventive Intervention with Ketogenic Diet Mediated by the Histone Acetylation of mGluR5 Promotor Region in Rat Parkinson’s Disease Model: A Dual-Tracer PET Study
Authors: Zhu Yuankai , Tang Xiangyu , Cheng Zhaoting , Dong Qingjian , Ruan Ge
Published: September 2022

Background and Objectives. The exact pathological mechanism of Parkinson’s disease (PD) remains elusive, and the existing therapies fail to reverse the disease progression. This study intended to explore the epigenetic anti-inflammatory mechanism of ketogenic diet (KD). Materials and Methods. The neuroprotective effect of ketosis state prior to the onset of PD (preventive KD, KDp) was compared with that receiving KD after the onset (therapeutic KD, KDt) in the lipopolysaccharide- (LPS-) induced rat PD model. A total of 100 rats were randomly assigned to the following 4 groups: sham, LPS, LPS + KDp, and LPS + KDt groups. Results. Significant dopamine deficient behaviors (rotational behavior and contralateral forelimb akinesia), upregulation of proinflammatory mediators (TNF-α, IL-1β, and IL-6), loss of dopaminergic neurons, reduction of mGluR5+ microglia cells, increase of TSPO+ microglia cells, reduction of H3K9 acetylation in the mGluR5 promoter region and mGluR5 mRNA expression, and decline in the phosphorylation levels of Akt/GSK-3β/CREB pathway were observed after the intervention of LPS (

P
<
0.01

). TSPO and DAT PET imaging revealed the increased uptake of 18F-DPA-714 in substantia nigra and decreased uptake of 18F-FP-CIT in substantia nigra and striatum in LPS-treated rats (

P
<
0.001

). These impairments were alleviated by the dietary intervention of KD, especially with the strategy of KDp (

P
<
0.05

). Conclusions. The anti-inflammatory effect of KD on PD was supposed to be related to the modulation of Akt/GSK-3β/CREB signaling pathway mediated by the histone acetylation of mGluR5 promotor region. The KD intervention should be initiated prior to the PD onset in high-risk population to achieve a more favorable outcome.
Evaluating and Identifying Climatic Design Features in Traditional Iranian Architecture for Energy Saving (Case Study of Residential Architecture in Northwest of Iran)
Authors: Nakhaee Sharif Amirmasood , Keshavarz Saleh Sanaz , Afzal Sadegh , Shoja Razavi Niloofar , Fadaei Nasab Mozhdeh , Kadaei Samireh
Published: September 2022

In the last decades, researchers have been considering some fundamental issues such as energy saving, global warming, greenhouse emissions, and non‐renewable energy to make models of house environmental standards to achieve a suitable consumption pattern for saving energy. In architecture, using natural energy is one of the essential pillars of design because it was one of the criteria of designing, which was considered on climate and geography, and it has been a high performance of climate adaptation in the modeling of traditional houses. In this research, Azerbaijan (located in northwestern Iran) is selected to evaluate the practical features of traditional Iranian houses designed in the cold climate, and criteria for developing sensible solutions to achieve a suitable design model for energy saving are provided. The primary purpose of this paper is to evaluate and identify the features of climate design in traditional houses in a cold climate, which are suitable residential buildings for energy management, and to identify the components affecting energy saving. The data collection method is based on checklists, observation, considering the orientation, density, solar radiation angle in the region, documentary, analysis of maps, and adaptation of the architectural plan of the studied houses with the pattern of solar radiation in the area. This research discusses the design criteria for future structures and their adaptable measures based on the obtained results. Finally, it is declared that the traditional architectural design model follows the region’s climatic conditions, and considering the current climate and energies, traditional houses were designed; therefore, the best model for maximum use of available energy is climatic design. As a result, suggestions are made regarding residential architecture design to save energy.
Airway Management of Esophageal Atresia and Tracheoesophageal Fistula Combined with Anal Atresia
Authors: Zhou Jieshu , Li Hao , Lin Xuemei
Published: September 2022

Esophageal atresia (EA) associated with tracheoesophageal fistula (TEF) is a common congenital airway anomaly and may be associated with other gastrointestinal abnormalities. Neonates with EA/TEF are at high risk of gastrointestinal distension due to the shunting of air via the fistula, leading to progressive diaphragmatic elevation and regurgitation of the gastrointestinal contents. EA/TEF associated with anal atresia in a neonate makes airway management even more challenging particularly when managed for the repair of TEF through thoracotomy. Here, we report a case where we succeeded in conducting the flexible bronchoscopy insertion through a laryngeal mask to block the fistula by bronchial blocker under spontaneous breathing.
Application of Nanomaterials in the Field of New Energy Environment and Economic Benefit Analysis
Authors: Xu Shuai , An Haoying , Dai Jiahai
Published: September 2022

The depletion of traditional fossil energy and the air pollution caused by the burning of fossil fuels have forced science authors from all over the world to gradually turn their attention to the development and utilization of renewable energy. However, limited by the defects of less exposed active surface and slow diffusion of traditional materials, nanomaterials have advantages that traditional materials cannot match and can significantly improve the performance of energy conversion and storage devices. Based on this, this paper studies the application and economic benefits of nanomaterials in the field of new energy and environment and provides a new idea for the development direction of nanomaterials in the field of new energy and environment. This article selects three different survey objects to correctly measure the energy consumed by the three groups of experimental vehicles traveling 1 km, 5 km, 10 km, and 20 km and the content of substances that pollute the environment. At the same time, the three traditional electrodes are connected. The electrochemical workstation of the system measures the electrochemical performance of nanomaterials to analyze the application of nanomaterials in the field of new energy and environment and the economic benefits brought by them. The experiment proves that AgPdMo/MoS2 NWs has the smallest charge transfer resistance of 259.3 Ω, the smallest impedance, and the fastest electron transfer rate. The charge transfer resistances of MoS2 NSs, AgPd NWs, and AgPdMo NWs are 37079 Ω, 398.4 Ω, and 916.6 Ω.
Implementation of Language Appropriateness in Intercultural Communication Based on Distributed Control System
Authors: Liu Li , Xue Duan , Zhang Yijia , Huo Xingying
Published: September 2022

In recent years, with the increasingly close international communication, the importance of intercultural communication language has become more and more important. Proficiently mastering the appropriateness of intercultural communication language is an important part of national etiquette, which can represent the self-cultivation of a country. However, in reality, many students cannot master language appropriateness very well. Therefore, it is necessary to retrieve and classify intercultural communication languages based on distributed control systems, so as to improve language appropriateness and national comprehensive strength. This study proposed a language processing method based on distributed computing, which solved the problems of a large amount of computation and low accuracy in traditional language processing methods and simply designed a distributed control system, which could process language for intercultural communication. The results of this study showed that the language appropriateness score of the respondents was 53 points before the system developed in this study was used. After using the system developed in this study to retrieve and correct the language errors, the language appropriateness score of the respondents was 95 points, which was 42 points higher and also showed that the system developed in this study could effectively improve Language appropriateness.
Analyzing the Network Evaluation Model in Mobile Learning Environment to Monitor Different Capabilities of Students
Authors: Hu Yanhua
Published: September 2022

In light of the current ideological and political teaching quality’s poor impact, this paper proposes the construction and research method of a network ideological and political teaching quality evaluation model from the perspective of school-enterprise cooperation, optimises the network ideological and political teaching model using the Internet technology, constructs the teaching quality evaluation index and algorithm, and realizes the optimization of the ideopolitical teaching model. Finally, it is confirmed by experiments. According to the viewpoint of school endeavor collaboration, the organization’s philosophical and political quality evaluation model has higher practicability during the time spent in commonsense application and completely meets the examination prerequisites.
Impact of Motivational Enhanced Adherence Counseling and Point-of-Care Viral Load Monitoring on Viral Load Outcome in Women on Life-Long ART: A Randomized Pilot Study
Authors: Mutambanengwe-Jacob Mercy T. , Maponga Charles C. , Amico K. Rivet , Ngara Bernard , Yende-Zuma Nonhlanhla , Chawana Tariro D. , Nematadzira Teacler , Gumbo Justice F. , Goverayi Tendayi , Matibe Petronella , Malunda Bernadette V. , Aizire Jim , Taha Taha E. , Fowler Mary G. , Stranix-Chibanda Lynda
Published: September 2022

We piloted the combined effectiveness of point-of-care viral load monitoring plus motivational enhanced adherence counseling (intervention) compared with routine care (control) in women identified at risk of virologic failure in the PROMOTE study in Zimbabwe. In an unblinded randomized study, consenting women with last viral load ≥200 copies/ml and/or pill count outside 90–110% range were randomized 1 : 1 to receive the intervention or continue routine care, comprising laboratory-based VL monitoring and standard EAC, from trained nurses and counsellors. Viral load was measured 0, 3, 6, and 12 months after enrolment. We compared viral suppression <200 copies/ml at 6 and 12 months between the arms through Fisher’s exact test and sought associated factors by logistic regression with a 95% confidence interval (CI). Between December 2018 and July 2019, 50 women were enrolled (25 intervention and 25 controls) and followed until November 2020. At entry, 60% of the women were virally suppressed, 52% intervention vs. 68% control arm. Viral suppression was balanced between the two arms (

p

value = 0.248). At month 6 post study entry (primary endpont), 64% of the women retained in care were virally suppressed, 54% intervention vs. 76% control arm (

p

value = 0.124). At month12 post study entry (secondary endpoint), 69% of the women retained in care were virally suppressed, 67% intervention vs. 71% control arm women (

p

value = 0.739). More intervention women completed all scheduled sessions by month 6. Control group women were more likely to be virally suppressed at both timepoints. Only 25% had treatment switch by 12 months. Despite intense adherence support and viral load monitoring, sustained viral suppression remained elusive in women identified at risk of viral failure. These findings highlight the continued need for effective adherence intervention for women with unsuppressed HIV viral loads, efficient treatment switch strategies, as well as drug level monitoring.
The Connotation and Construction Path of the Principle of Good Faith from the Perspective of Civil and Commercial Law
Authors: Ma Li
Published: September 2022

In order to explore the connotation and construction path of the principle of good faith from the perspective of civil and commercial law, this paper combines intelligent algorithms to analyze the connotation and construction path of the principle of good faith from the perspective of civil and commercial law. Moreover, this paper presents a topology network specially used to improve the energy absorption efficiency of the isolation resistor used in the power divider and theoretically analyzes the effectiveness of the isolation topology. In addition, this paper further optimizes the topology of the two-way broadband filter power divider and builds a simulation model based on the basic content of the principle of good faith. From the research results, the simulation model proposed in this paper can play a certain role in the connotation and construction path of the principle of good faith from the perspective of civil and commercial law. On this basis, some suggestions on the connotation and construction path of the principle of good faith are put forward.
Leukemia Inhibitory Factor Facilitates Self-Renewal and Differentiation and Attenuates Oxidative Stress of BMSCs by Activating PI3K/AKT Signaling
Authors: Liang Youde , Zhou Ruiping , Liu Xin , You Lin , Chen Chang , Ye Xiaoling , Wei Wei , Liu Jie , Dai Jiawei , Li Kaixiong , Zhao Xiangxiang
Published: September 2022

Objective. Transplantation of bone marrow-derived mesenchymal stem cells (BMSCs) remains a hopeful therapeutic approach for bone defect reconstruction. Herein, we investigated the effects and mechanisms of leukemia inhibitory factor (LIF) in the function and viability of hypoxic BMSCs as well as bone defect repair. Methods. The effects of LIF on apoptosis (flow cytometry, TUNEL staining), mitochondrial activity (JC-1 staining), proliferation (colony formation, EdU staining), and differentiation (CD105, CD90, and CD29 via flow sorting) were examined in hypoxic BMSCs. LIF, LIFR, gp130, Keap1, Nrf2, antioxidant enzymes (SOD1, catalase, GPx-3), bone-specific matrix proteins (ALP, BSP, OCN), PI3K, and Akt were detected via immunoblotting or immunofluorescent staining. BMSCs combined with biphasic calcium phosphate scaffolds were implanted into calvarial bone defect mice, and the therapeutic effect of LIF on bone defect was investigated. Results. Hypoxic BMSCs had increased apoptosis and oxidative stress and reduced mitochondrial activity. Additionally, LIF, LIFR, and gp130 were upregulated and PI3K/Akt activity was depressed in hypoxic BMSCs. Upregulated LIF alleviated apoptosis and oxidative stress and heightened mitochondrial activity and PI3K/Akt signaling in hypoxic BMSCs. Additionally, LIF overexpression promoted self-renewal and osteogenic differentiation of BMSCs with hypoxic condition. Mechanically, LIF facilitated self-renewal and differentiation as well as attenuated oxidative stress of BMSCs through enhancing PI3K/AKT signaling activity. Implantation of LIF-overexpressed BMSC-loaded BCP scaffolds promoted osteogenesis as well as alleviated oxidative stress and apoptosis through PI3K/Akt signaling. Conclusion. Our findings demonstrate that LIF facilitates self-renewal and differentiation and attenuates oxidative stress of BMSCs by PI3K/AKT signaling.
Experimental Analysis of Mechanical and Thermal Characteristics of Luffa/Epoxy Polymer Composite under the Influence of Nanosilica
Authors: Saminathan Rajasekaran , Hadidi Haitham , Fageehi Yahya Ali , Manoj Kumar P. , Venkatasudhahar M. , Ankit  , Ram S. , Gebreyohannes Dawit Tafesse
Published: September 2022

In the current work, the experimentations have been accomplished to assess the impact of diffusing nanosilica particles in epoxy matrix on the mechanical and thermal performance of the luffa fibre reinforced epoxy composite material. The matrix and fibre composition are fixed as 80 : 20 throughout the study, and the nanosilica is disbanded in diversified volume fractions of 0%, 0.5%, 1.0%, 1.5%, 2.0%, and 2.5%, respectively, while preparing luffa/epoxy/nanosilica (LES) composite samples. The mechanical characteristics, such as tensile, flexural, and impact behaviour of the composite, and the thermal properties, namely, thermal stability and thermal conductivity, are examined for the LES samples. The experiments are accomplished as per the ASTM standards. The results revealed that the assimilation of nanosilica particles in epoxy has improved the mechanical and thermal characteristics of the composite significantly. The tensile, flexural, and impact strength of the composite have been amended by 157.58%, 66.9%, and 16.5% with 1.5% addition of nanosilica in epoxy. Similarly, thermal conductivity of the composite is improved by 47.53% with the dispersion of 2.5% nanosilica in epoxy matrix. In addition, the thermal stability of the LES composite samples is substantially improved while disbanding nanosilica in epoxy matrix. However, the better results are reported for the LES composites containing 1.5% nanosilica content, compared to the next higher volume fractions of nanosilica content in epoxy matrix.