bluuebunny/crossref_metadata_2025 huggingface dataset:
Methods for Inferring Route Choice of Commuting Trip From Mobile Phone Network Data
Authors: Sakamanee Pitchaya , Phithakkitnukoon Santi , Smoreda Zbigniew , Ratti Carlo
Published: May 2020

For billing purposes, telecom operators collect communication logs of our mobile phone usage activities. These communication logs or so called CDR has emerged as a valuable data source for human behavioral studies. This work builds on the transportation modeling literature by introducing a new approach of crowdsource-based route choice behavior data collection. We make use of CDR data to infer individual route choice for commuting trips. Based on one calendar year of CDR data collected from mobile users in Portugal, we proposed and examined methods for inferring the route choice. Our main methods are based on interpolation of route waypoints, shortest distance between a route choice and mobile usage locations, and Voronoi cells that assign a route choice into coverage zones. In addition, we further examined these methods coupled with a noise filtering using Density-Based Spatial Clustering of Applications with Noise (DBSCAN) and commuting radius. We believe that our proposed methods and their results are useful for transportation modeling as it provides a new, feasible, and inexpensive way for gathering route choice data, compared to costly and time-consuming traditional travel surveys. It also adds to the literature where a route choice inference based on CDR data at this detailed level—i.e., street level—has rarely been explored.
Geospatial Serverless Computing: Architectures, Tools and Future Directions
Authors: Bebortta Sujit , Das Saneev Kumar , Kandpal Meenakshi , Barik Rabindra Kumar , Dubey Harishchandra
Published: May 2020

Several real-world applications involve the aggregation of physical features corresponding to different geographic and topographic phenomena. This information plays a crucial role in analyzing and predicting several events. The application areas, which often require a real-time analysis, include traffic flow, forest cover, disease monitoring and so on. Thus, most of the existing systems portray some limitations at various levels of processing and implementation. Some of the most commonly observed factors involve lack of reliability, scalability and exceeding computational costs. In this paper, we address different well-known scalable serverless frameworks i.e., Amazon Web Services (AWS) Lambda, Google Cloud Functions and Microsoft Azure Functions for the management of geospatial big data. We discuss some of the existing approaches that are popularly used in analyzing geospatial big data and indicate their limitations. We report the applicability of our proposed framework in context of Cloud Geographic Information System (GIS) platform. An account of some state-of-the-art technologies and tools relevant to our problem domain are discussed. We also visualize performance of the proposed framework in terms of reliability, scalability, speed and security parameters. Furthermore, we present the map overlay analysis, point-cluster analysis, the generated heatmap and clustering analysis. Some relevant statistical plots are also visualized. In this paper, we consider two application case-studies. The first case study was explored using the Mineral Resources Data System (MRDS) dataset, which refers to worldwide density of mineral resources in a country-wise fashion. The second case study was performed using the Fairfax Forecast Households dataset, which signifies the parcel-level household prediction for 30 consecutive years. The proposed model integrates a serverless framework to reduce timing constraints and it also improves the performance associated to geospatial data processing for high-dimensional hyperspectral data.
Identifying the Socio-Spatial Logics of Foreclosed Housing Accumulated by Large Private Landlords in Post-Crisis Catalan Cities
Authors: Gutiérrez Aaron , Domènech Antoni
Published: May 2020

The article analyses the socio-spatial logic behind the accumulation of foreclosed housing in the hands of large private landlords in the neighbourhoods of all the Catalan cities with over 100,000 inhabitants. Spatial regression and clustering techniques are applied to identify the determinants of the concentration patterns of 10,725 housing units in these cities. The socioeconomic variables, such as income level, percentage of foreign population, level of studies or percentage of unemployed residents, are identified as key explanatory factors of clustering of foreclosures in working-class neighbourhoods. A high presence of previously mortgaged homes is a variable especially relevant in the case of working-class neighbourhoods, but it has no incidence in the case of the medium-high class neighbourhoods. Our findings provide a detailed urban geography of the housing accumulated by banks which, at the same time, correspond to areas in which the vulture funds are focusing their business in the present and in the forthcoming years. New evidences of the spatial logic of the housing crisis and detailed information for the understanding of the new scenarios that have emerged during the post-crisis phase are revealed.
Small RNA and Transcriptome Sequencing Reveals miRNA Regulation of Floral Thermogenesis in Nelumbo nucifera
Authors: Zou Yu , Chen Guanglong , Jin Jing , Wang Ying , Xu Meiling , Peng Jing , Ding Yi
Published: May 2020

The sacred lotus (Nelumbo nucifera Gaertn.) can produce heat autonomously and maintain a relatively stable floral chamber temperature for several days when blooming. Floral thermogenesis is critical for flower organ development and reproductive success. However, the regulatory role of microRNA (miRNA) underlying floral thermogenesis in N. nucifera remains unclear. To comprehensively understand the miRNA regulatory mechanism of thermogenesis, we performed small RNA sequencing and transcriptome sequencing on receptacles from five different developmental stages. In the present study, a total of 172 known miRNAs belonging to 39 miRNA families and 126 novel miRNAs were identified. Twenty-nine thermogenesis-related miRNAs and 3024 thermogenesis-related mRNAs were screened based on their expression patterns. Of those, seventeen differentially expressed miRNAs (DEMs) and 1765 differentially expressed genes (DEGs) had higher expression during thermogenic stages. The upregulated genes in the thermogenic stages were mainly associated with mitochondrial function, oxidoreductase activity, and the energy metabolism process. Further analysis showed that miR156_2, miR395a_5, miR481d, and miR319p may play an important role in heat-producing activity by regulating cellular respiration-related genes. This study provides comprehensive miRNA and mRNA expression profile of receptacle during thermogenesis in N. nucifera, which advances our understanding on the regulation of floral thermogenesis mediated by miRNA.
Resveratrol Induces Expression of Metabolic and Antioxidant Machinery and Protects Tilapia under Cold Stress
Authors: Wang Min-Chen , Wang Yu-Chun , Peng Hui-Wen , Hseu Jinn-Rong , Wu Guan-Chung , Chang Ching-Fong , Tseng Yung-Che
Published: May 2020

Exposures to low ambient temperature require ectothermic fish to not only adjust their metabolic machinery but also to mount protective responses against oxidative stress. In this study, we tested whether diets supplemented with resveratrol (RSV), a naturally occurring polyphenol known to stimulate metabolic and protective responses in various animals, would be beneficial to tilapia (Oreochromis mossambicus) under hypothermic challenge. Feeding tilapia with RSV-supplemented diet promoted liver expression of sirtuins and their known targets, including metabolic/antioxidative enzymes. After exposure to 15 °C cold conditions for three days, the oxygen–nitrogen (O:N) ratio was decreased in the control-diet-fed tilapia but not in their RSV-fed counterparts. Moreover, at 27 °C, RSV-fed tilapia showed significantly higher prolonged swim speed compared with controls. RSV feeding produced no significant effect on upper and bottom layer preference between the control- and RSV-treated tilapia at either 27 °C or 15 °C. Together, these findings suggest that RSV stimulates beneficial metabolic/antioxidative adjustments in teleosts and may serve as a valuable feed supplement for tropical fish exposed to cold stress during winter.
An Immunological Glance on Pancreatic Ductal Adenocarcinoma
Authors: Melzer Michael Karl , Arnold Frank , Stifter Katja , Zengerling Friedemann , Azoitei Ninel , Seufferlein Thomas , Bolenz Christian , Kleger Alexander
Published: May 2020

Pancreatic ductal adenocarcinoma (PDAC) has still a dismal prognosis. Different factors such as mutational landscape, intra- and intertumoral heterogeneity, stroma, and immune cells impact carcinogenesis of PDAC associated with an immunosuppressive microenvironment. Different cell types with partly opposing roles contribute to this milieu. In recent years, immunotherapeutic approaches, including checkpoint inhibitors, were favored to treat cancers, albeit not every cancer entity exhibited benefits in a similar way. Indeed, immunotherapies rendered little success in pancreatic cancer. In this review, we describe the communication between the immune system and pancreatic cancer cells and propose some rationale why immunotherapies may fail in the context of pancreatic cancer. Moreover, we delineate putative strategies to sensitize PDAC towards immunological therapeutics and highlight the potential of targeting neoantigens.
Clotting Factors in COVID-19: Epidemiological Association and Prognostic Values in Different Clinical Presentations in an Italian Cohort
Authors: Di Micco Pierpaolo , Russo Vincenzo , Carannante Novella , Imparato Michele , Rodolfi Stefano , Cardillo Giuseppe , Lodigiani Corrado
Published: May 2020

Introduction: A novel highly pathogenic human coronavirus able to induce severe acute respiratory syndrome (SARS) has been recently recognized as the cause of the coronavirus disease 2019 (COVID-19) outbreak, which has spread rapidly from China to other countries. Little is known about laboratory prognostic markers in COVID-19 patients. The aim of our study was to describe the basic clotting parameters in COVID-19 patients and their prognostic role in different clinical forms of the disease. Material and Methods: We enrolled 67 COVID-19 patients admitted to the Emergency Department. A cohort of 67 age- and sex-matched non-COVID-19 patients with acute respiratory illness was used as a control group. For all patients, platelet count (PLT), prothrombin time (PT), activated thromboplastin time (aPTT), C-reactive protein (PCR), fibrinogen, and D-dimer were determined. The COVID-19 population was divided in two groups according to the presence or absence of SARS. The clotting factors values were compared between the groups. Results: At admission, the COVID-19 patients showed statistically significant increased levels of fibrinogen (601.5 (480–747) vs. 455 (352.5–588.5) mg/dL; p = 0.0000064), and a higher percentage of patients had fibrinogen levels >400 mg/dL (86% vs.58%; p = 0.0054) compared to the control group. The levels of fibrinogen were higher in COVID-19 patients with SARS compared to those without SARS (747 (600.0–834.0) vs. 567 (472.5–644.50); p = 0.0003). Conclusion: Fibrinogen seems to increase early in COVID-19 patients and may be used as a risk stratification marker for the early detection of a subgroup of COVID-19 patient at increased risk to develop SARS, who might benefit from a different and thorough clinical surveillance and treatment.
Aggrecan Turnover in Women with Rheumatoid Arthritis Treated with TNF-α Inhibitors
Authors: Szeremeta Anna , Jura-Półtorak Agnieszka , Zoń-Giebel Aleksandra , Kopeć-Mędrek Magdalena , Kucharz Eugeniusz Józef , Olczyk Krystyna
Published: May 2020

This study was performed to evaluate the effects of 15-month anti-tumor necrosis factor α (anti-TNF-α) therapy on the aggrecan turnover of female rheumatoid arthritis (RA) patients. Serum was obtained from healthy subjects and female RA patients treated with TNF-α inhibitors (TNFαI) in combination with methotrexate. We measured serum levels of aggrecan chondroitin sulfate 846 epitope (CS846), aggrecan fragments (AGC), disintegrin and metalloproteinase with thrombospondin motifs-4 (ADAMTS-4) and 5 (ADAMTS-5), as well as their natural inhibitor, known as tissue inhibitor of matrix metalloproteinase-3 (TIMP-3), using immunoassay methods. Serum levels of CS846, AGC, ADAMTS-4, ADAMTS-5 and TIMP-3 were higher in female patients with RA before the treatment in comparison to healthy subjects. Ratio of ADAMTS-5 to TIMP-3 was significantly higher in RA women than in controls, whereas ADAMTS-4/TIMP-3 ratio did not differ from that in controls. During the anti-TNF-α therapy, the serum levels of 846 epitope increased, whereas levels of AGC decreased in female RA patients. Furthermore, 15 months of treatment with TNFαI downregulated serum levels of both ADAMTS, without any effect on TIMP-3 levels. These changes were accompanied by significantly reduced ratios of ADAMTS to TIMP-3. According to our results, anti-TNF-α therapy has a beneficial impact on aggrecan remodeling during RA.
Listening in Noise Remains a Significant Challenge for Cochlear Implant Users: Evidence from Early Deafened and Those with Progressive Hearing Loss Compared to Peers with Normal Hearing
Authors: Zaltz Yael , Bugannim Yossi , Zechoval Doreen , Kishon-Rabin Liat , Perez Ronen
Published: May 2020

Cochlear implants (CIs) are the state-of-the-art therapy for individuals with severe to profound hearing loss, providing them with good functional hearing. Nevertheless, speech understanding in background noise remains a significant challenge. The purposes of this study were to: (1) conduct a novel within-study comparison of speech-in-noise performance across ages in different populations of CI and normal hearing (NH) listeners using an adaptive sentence-in-noise test, and (2) examine the relative contribution of sensory information and cognitive–linguistic factors to performance. Forty CI users (mean age 20 years) were divided into “early-implanted” <4 years (n = 16) and “late-implanted” >6 years (n = 11), all prelingually deafened, and “progressively deafened” (n = 13). The control group comprised 136 NH subjects (80 children, 56 adults). Testing included the Hebrew Matrix test, word recognition in quiet, and linguistic and cognitive tests. Results show poorer performance in noise for CI users across populations and ages compared to NH peers, and age at implantation and word recognition in quiet were found to be contributing factors. For those recognizing 50% or more of the words in quiet (n = 27), non-verbal intelligence and receptive vocabulary explained 63% of the variance in noise. This information helps delineate the relative contribution of top-down and bottom-up skills for speech recognition in noise and can help set expectations in CI counseling.
Understanding the Deterioration of Gait, Postural Control, Lower Limb Strength and Perceived Fatigue Across the Disability Spectrum of People with Multiple Sclerosis
Authors: Moreno-Navarro Pedro , Gomez-Illán Ramón , Carpena-Juan Carmen , P. Sempere Ángel , Vera-Garcia Francisco J. , Barbado David
Published: May 2020

Disability progression is a prominent feature of multiple sclerosis (MS). However, little is known about the extent to which physical condition parameters and perceived fatigue evolve during the disease. We analyzed how strength, balance, core stability and perceived fatigue differ among different cohorts of people with MS (PwMS) with different disability degrees and how these contribute to patients’ gait speed and functional mobility. Sixty-three PwMS divided into three groups according to the “Expanded Disability Status Scale” (MS1: EDSS ≤ 1.5; MS2: 2 ≤ EDSS ≤ 3.5; MS3: 4 ≤ EDSS ≤ 6) and 22 healthy controls (HC) participated in this study. MS1 showed lower balance and hip strength compared to HC. MS2 showed lower balance, core stability, gait speed, and functional mobility than MS1. MS3 showed lower gait speed, functional mobility, balance, and knee flexion strength than MS2. No between-group differences were observed in perceived fatigue. Relative weight analysis showed that strength, balance and core stability explained 60%–70% of the variance in gait speed and functional mobility. The decline of each parameter did not evolve at the same rate across the different stages of the disease, being knee flexion strength and balance the most influential factors in the disability progression. Overall, these results provide useful information to guide exercise prescription at different stages of MS.
Effects of a Multicomponent Exercise Program in Physical Function and Muscle Mass in Sarcopenic/Pre-Sarcopenic Adults
Authors: Makizako Hyuma , Nakai Yuki , Tomioka Kazutoshi , Taniguchi Yoshiaki , Sato Nana , Wada Ayumi , Kiyama Ryoji , Tsutsumimoto Kota , Ohishi Mitsuru , Kiuchi Yuto , Kubozono Takuro , Takenaka Toshihiro
Published: May 2020

This study aimed to assess the effects of a multicomponent exercise program on physical function and muscle mass in older adults with sarcopenia or pre-sarcopenia. Moreover, we aim to standardize the exercise program for easy incorporation in the daily life of community-dwelling older adults as a secondary outcome. A single-blind randomized controlled trial was conducted with individuals (≥60 years) who had sarcopenia or pre-sarcopenia (n = 72). Participants were randomly assigned to the exercise and control groups. The exercise program consisted of 12 weekly 60-min sessions that included resistance, balance, flexibility, and aerobic training. Outcome measures were physical function and muscle mass. Assessments were conducted before and immediately after the intervention. Among the 72 participants (mean age: 75.0 ± 6.9 years; 70.8% women), 67 (93.1%) completed the trial. Group-by-time interactions on the chair stand (p = 0.02) and timed “up and go” (p = 0.01) tests increased significantly in the exercise group. Although the exercise group showed a tendency to prevent loss of muscle mass, no significant interaction effects were observed for cross-sectional muscle area and muscle volume. The 12-week exercise program improved physical function in the intervention group. Although it is unclear whether the program is effective in increasing muscle mass, a multicomponent exercise program would be an effective treatment for physical function among older adults with sarcopenia.
High Frequency of Undiagnosed Psychiatric Disorders in Inflammatory Bowel Diseases
Authors: Marafini Irene , Longo Lucia , Miri Lavasani Damun , Rossi Rodolfo , Salvatori Silvia , Pianigiani Federica , Calabrese Emma , Siracusano Alberto , Di Lorenzo Giorgio , Monteleone Giovanni
Published: May 2020

Inflammatory bowel diseases (IBD) are associated with comorbid depressive and anxiety disorders, but a significant proportion of IBD patients with psychiatric disorders (PsychD) remain undiagnosed and untreated. The aim of this study was to assess the frequency and type of undiagnosed PsychD in IBD patients. Two hundred and thirty-seven adult IBD (136 Crohn’s disease (CD) and 101 ulcerative colitis (UC)) outpatients were consecutively recruited at a single university hospital centre between January 2018 and June 2019. After a scheduled follow-up visit for IBD clinical evaluation, participants underwent a semi-structured interview with a trained psychiatrist. One hundred and fourteen (48%) IBD patients had at least one PsychD, and in 67 (59%) of them, a diagnosis was made for the first time during the study. The remaining 47 (41%) patients had received a previous psychiatric diagnosis, but in only six cases was it consistent with the diagnosis made during the study. PsychD were equally distributed in CD (72/136, 53%) and UC (42/101, 42%), and mostly represented by mood disorders (54/114, 47%) and anxiety (27/114, 24%) disorders. PsychD were not related to the disease severity, phenotype or localisation of IBD, even though having three or more concomitant PsychD was associated with more severe disease in CD. Our data indicate that undiagnosed PsychD are common in IBD and highlight the importance of considering psychiatric evaluation in the management of IBD patients.
Urine Proteomic Study in OAB Patients—Preliminary Report
Authors: Futyma Konrad , Nowakowski Łukasz , Ziętek-Strobl Alicja , Kamińska Aleksandra , Taoussi Nadia , Rechberger Tomasz
Published: May 2020

Overactive bladder (OAB) is defined by International Urogynecological Association (IUGA)/ International Continence Society (ICS) as urinary urgency, usually accompanied by frequency and nocturia, with or without urgency urinary incontinence, in the absence of urinary tract infection (UTI) or other obvious pathology. The pathophysiology of OAB is not well understood, however a number of different proteins and cytokines including vascular cell adhesion molecule-1 (VCAM-1) were found to be important in regulating structural integrity of the bladder wall. Proteome analysis may thus provide significant information with regard to OAB and may help in discovering novel diagnostic disease biomarkers. Sixteen Caucasian women aged 32–78 were included in the study. Patients were placed within 2 groups: OAB group (n = 8) and control group (n = 8). Urine samples were collected, immediately preserved in a protease inhibitor mixture, and frozen at −80 ℃. All samples were then further processed according to the isobaric tags for relative and absolute quantification (iTRAQ) manual. Proteins were labeled and analyzed in the mass spectrometer conjugated with liquid chromatograph (data are available via ProteomeXchange with the identifier PXD017799). There were no statistically significant differences in demographic data between control and OAB groups. VCAM-1 was the only protein that reached statistical significance as a differentiating protein in both of our experiments assessing the proteomic constitution in OAB patients. Studies involving a larger group of patients may provide further information on urinary bladder proteomics.
Silver and Graphenic Carbon Nanostructures Differentially Influence the Morphology and Viability of Cardiac Progenitor Cells
Authors: Hotowy Anna , Grodzik Marta , Zielińska-Górska Marlena , Chojnacka Natalia , Kurantowicz Natalia , Dyjak Sławomir , Strojny Barbara , Kutwin Marta , Chwalibog André , Sawosz Ewa
Published: May 2020

The characteristic features of nanomaterials provide rich opportunities for a broad range of applications due to their different physicochemical properties. Nanocolloidal silver and graphenic carbon materials differ in most physicochemical characteristics, except for their nanodimensions. Since there is a growing demand for stem cell therapies for coronary disorders, examining cardiac progenitor cells (CPC) in terms of their response to nanostructure treatment seems to be a reasonable approach. Morphological studies and viability assessments were performed with CPC in vitro, treated with small concentrations of silver nanoparticles (AgNP), hierarchical nanoporous graphenic carbon (HNC) and their mixtures. A viability test confirmed the morphological assessment of CPC treated with AgNP and HNC; moreover, the action of both nanomaterials was time-dependent and dose-dependent. For AgNP, between the two of the applied concentrations lies a border between their potential beneficial effect and toxicity. For HNC, at a lower concentration, strong stimulation of cell viability was noted, whereas a higher dosage activated their differentiation. It is necessary to perform further research examining the mechanisms of the action of AgNP and especially of unexplored HNC, and their mixtures, on CPC and other cells.
Polyanilines as New Sorbents for Hydrocarbons Removal from Aqueous Solutions
Authors: Della Pina Cristina , De Gregorio Maria Antonietta , Dellavedova Pierluisa , Falletta Ermelinda
Published: May 2020

Water remediation from hydrocarbons is crucial to reduce health risks. Numerous costly and, sometimes, sophisticated methods were proposed over the years. Herein, an innovative green procedure for porous polyanilines preparation is reported. Polyaniline (PANI) was synthesized by three different approaches ranging from traditional to more eco-friendly ones. Thermal, optical and morphological features of the resulting materials were investigated along with their surface properties. Finally, PANIs were tested as sorbents for hydrocarbons removal from waterbodies. Although an overall fast and high sorption efficiency is always observed, the effective hydrocarbons abatement performed by ‘green’ PANIs is particularly welcome in the context of environmental protection. Moreover, the sorption efficiency retention after five-run recycling tests suggests potential applications in wastewater remediation.
Introducing Fractal Dimension for Interlaminar Shear and Tensile Strength Assessment of Mechanically Interlocked Polymer–Metal Interfaces
Authors: Saborowski Erik , Steinert Philipp , Dittes Axel , Lindner Thomas , Schubert Andreas , Lampke Thomas
Published: May 2020

The interlaminar strength of mechanically interlocked polymer–metal interfaces is strongly dependent on the surface structure of the metal component. Therefore, this contribution assesses the suitability of the fractal dimension for quantification of the surface structure, as well as interlaminar strength prediction of aluminum/polyamide 6 polymer–metal hybrids. Seven different surface structures, manufactured by mechanical blasting, combined mechanical blasting and etching, thermal spraying, and laser ablation, are investigated. The experiments are carried out on a butt-bonded hollow cylinder testing method that allows shear and tensile strength determination with one specific specimen geometry. The fractal dimension of the metal surfaces is derived from cross-sectional images. For comparison, the surface roughness slope is determined and related to the interlaminar strength. Finally, a fracture analysis is conducted. For the investigated material combination, the experimental results indicate that the fractal dimension is an appropriate measure for predicting the interlaminar strength.
Mathematical Model on Gravitational Electro-Magneto-Thermoelasticity with Two Temperature and Initial Stress in the Context of Three Theories
Authors: Abo-Dahab Sayed M. , El-Bary Alaa A. , Al-Hadeethi Yas , Alkashif Mohamed
Published: May 2020

The main aim of this paper is to study two temperature thermoelasticity in a generalization form to solve the half-space problem of two dimensions under gravity, perturbed magnetic field, and initial stress. The fundamental equations are solved considering a new mathematical technique under Lord-Şhulman (LS), Green-Naghdi (GN type III) and three-phase-lag (3PHL) theories to investigate displacement, stress components, and temperature distribution. The results obtained by the three theories, i.e., (LS), (GN type III), and (3PHL), considering the absence and the presence of gravity, initial stress, and magnetic field have been compared. The results were numerically calculated and graphically displayed to exhibit the physical meaning of the phenomenon and the external parameters’ effect. A comparison has been presented between the results obtained in the absence and the presence of the external considered parameters and with the previously obtained results by other researchers.
Multi-Stack Lifetime Improvement through Adapted Power Electronic Architecture in a Fuel Cell Hybrid System
Authors: Bahrami Milad , Martin Jean-Philippe , Maranzana Gaël , Pierfederici Serge , Weber Mathieu , Meibody-Tabar Farid , Zandi Majid
Published: May 2020

To deal with the intermittency of renewable energy resources, hydrogen as an energy carrier is a good solution. The Polymer Electrolyte Membrane Fuel Cell (PEMFC) as a device that can directly convert hydrogen energy to electricity is an important part of this solution. However, durability and cost are two hurdles that must be overcome to enable the mass deployment of the technology. In this paper, a management system is proposed for the fuel cells that can cope with the durability issue by a suitable distribution of electrical power between cell groups. The proposed power electronics architecture is studied in this paper. A dynamical average model is developed for the proposed system. The validation of the model is verified by simulation and experimental results. Then, this model is used to prove the stability and robustness of the control method. Finally, the energy management system is assessed experimentally in three different conditions. The experimental results validate the effectiveness of the proposed topology for developing a management system with which the instability of cells can be confronted. The experimental results verify that the system can supply the load profile even during the degradation mode of one stack and while trying to cure it.
Abstract Formulation of the Miura Transform
Authors: Iwata Yoritaka
Published: May 2020

Miura transform is known as the transformation between Korweg de-Vries equation and modified Korweg de-Vries equation. Its formal similarity to the Cole-Hopf transform has been noticed. This fact sheds light on the logarithmic type transformations as an origin of a certain kind of nonlinearity in the soliton equations. In this article, based on the logarithmic representation of operators in infinite-dimensional Banach spaces, a structure common to both Miura and Cole-Hopf transforms is discussed. In conclusion, the Miura transform is generalized as the transform in abstract Banach spaces, and it is applied to the higher order abstract evolution equations.
Recovery of Aromatics from Orange Juice Evaporator Condensate Streams by Reverse Osmosis
Authors: Destani Fitim , Naccarato Attilio , Tagarelli Antonio , Cassano Alfredo
Published: May 2020

The aim of this work was to analyze the potential of reverse osmosis (RO) membranes in the recovery and concentration of aroma compounds from orange juice evaporator condensate (EC) streams. Concentration experiments were performed by using three RO spiral-wound aromatic polyamide membranes (SG1812C-34D, SC1812C-34D and SE1812) with different NaCl rejections. The effect of transmembrane pressure, axial feed flowrate and volume concentration ratio (VCR) on permeate flux was studied. Rejections of the investigated membranes towards specific aroma compounds (octanol, α-terpineol, terpinen-4-ol, cis-carveol, karvon, linalool) in selected operating conditions were also evaluated. The concentrations of the aroma compounds were determined by gas chromatography coupled with mass spectrometry (GC-MS) using headspace solid-phase microextraction (HS-SPME) as a sample preparation approach. For all selected membranes, the permeate flux increased linearly by increasing the operating pressure from 5 to 25 bar; on the other hand, the feed flowrate did not have any significant effect on the permeate flux. High retention values towards aroma compounds (>80%) were measured for all selected membranes. However, the SC membrane showed the highest rejection values (>96%) and the best correlation between concentration factor of aroma compounds and VCR.
Recovery of Rubidium and Cesium Resources from Brine of Desalination through t-BAMBP Extraction
Authors: Chen Wei-Sheng , Lee Cheng-Han , Chung Yi-Fan , Tien Ko-Wei , Chen Yen-Jung , Chen Yu-An
Published: May 2020

50 billion cubic meters of brine every year creates ecological hazards to the environment. In order to reuse brine efficiently, rubidium and cesium were recovered in this experiment. On the other hand, the main impurities which were needed to be eliminated in brine were lithium, sodium, potassium, calcium, and magnesium. In the procedure, seawater was distilled and evaporated first to turn into simulated brine. Perchloric acid was then added into simulated brine to precipitate potassium perchlorate which could reduce the influence of potassium in the extraction procedure. After that, t-BAMBP and ammonia were separately used as extractant and stripping agent in the extraction and stripping procedures to get rubidium hydroxide solutions and cesium hydroxide solutions. Subsequently, they reacted with ammonium carbonate to get rubidium carbonate and cesium carbonate. In a nutshell, this study shows the optimal parameters of pH value to precipitate potassium perchlorate. Besides, pH value in the system, the concentration of t-BAMBP and ammonia, organic phase/aqueous phase ratio (O/A ratio), reaction time, and reaction temperature in solvent extraction step were investigated to get high purities of rubidium carbonate and cesium carbonate.
MetaboAnalystR 3.0: Toward an Optimized Workflow for Global Metabolomics
Authors: Pang Zhiqiang , Chong Jasmine , Li Shuzhao , Xia Jianguo
Published: May 2020

Liquid chromatography coupled to high-resolution mass spectrometry platforms are increasingly employed to comprehensively measure metabolome changes in systems biology and complex diseases. Over the past decade, several powerful computational pipelines have been developed for spectral processing, annotation, and analysis. However, significant obstacles remain with regard to parameter settings, computational efficiencies, batch effects, and functional interpretations. Here, we introduce MetaboAnalystR 3.0, a significantly improved pipeline with three key new features: (1) efficient parameter optimization for peak picking; (2) automated batch effect correction; and (3) more accurate pathway activity prediction. Our benchmark studies showed that this workflow was 20~100× faster compared to other well-established workflows and produced more biologically meaningful results. In summary, MetaboAnalystR 3.0 offers an efficient pipeline to support high-throughput global metabolomics in the open-source R environment.
Sulfide Rb-Sr, Re-Os and In Situ S Isotopic Constraints on Two Mineralization Events at the Large Hongnipo Cu Deposit, SW China
Authors: Lin Lujun , Chen Renyi , Pang Zhenshan , Chen Hui , Xue Jianling , Jia Hongxiang
Published: May 2020

The Hongnipo deposit, a newly discovered large copper deposit in the Kangdian copper belt, SW China, is hosted in the Paleoproterozoic Hekou Group. This deposit contains ~4200 Mt of Cu ores, with an average grade of 1.42 wt.% Cu. Orebodies occur mainly as stratiform, stratoid and lenticular forms. Mineralization consists predominantly of high grade vein-type ores and low grade laminated ores. Field relationships indicate vein-type mineralization crosscuts laminated mineralization and host rocks, indicating that there were at least two mineralization events during the formation of the deposit. Pyrite separates from the laminated ores yield a Rb-Sr isochron age of 1552 ± 80 Ma, with a highly radiogenic initial 87Sr/86Sr ratio of 0.71214 ± 0.00081, indicating a major contribution from the ore-hosting rocks. Sulfides from the laminated ores have δ34S values ranging from −1.8‰ to 11.4‰, with the vast majority in the range of 5.3‰ to 11.4‰, suggesting the mixed derivation of sulfur from seawater sulfates and magmatic fluids. Chalcopyrite separates from the vein-type ores have a Re-Os isochron age of 794.8 ± 7.9 Ma. The initial 187Os/188Os (2.8 ± 1.2) and γOs (+2202) values are slightly lower than the average values of continental crust, indicating a major metal source of the Hekou Group with minor mantle input. Sulfides from the vein-type ores have δ34S values that range from −10.3‰ to 4.0‰ and cluster between 0‰ to 2.2‰, which implies a significant contribution of magmatic-sourced sulfur with minor biogenic sulfur. Two major mineralization events have been identified. The Rb-Sr age of the laminated ores likely records a VMS mineralization event at ~1.6 Ga. The much younger Re-Os age is considered to represent the timing of an important mineralization event, which is likely related to the Neoproterozoic magmatism and/or metamorphism and represents a newly documented mineralization event to be targeted by exploration.
Auriferous Quartz Veining Due to CO2 Content Variations and Decompressional Cooling, Revealed by Quartz Solubility, SEM-CL and Fluid Inclusion Analyses (The Linglong Goldfield, Jiaodong)
Authors: Wei Qing , Fan Hongrui , Pironon Jacques , Liu Xuan
Published: May 2020

Quartz is the most common gangue mineral in hydrothermal veins. Coupled with capacities of hosting fluid inclusions and recording varieties of microtextures, its solubility behavior may provide unparalleled insights into hydrothermal processes. In this study, the Linglong goldfield in Jiaodong is targeted to investigate gold-producing quartz veining process. Scanning electron microscope (SEM)-cathodoluminescence (CL) imaging uncovered three episodes of quartz deposition, intervened by an episode of quartz dissolution. Based on newly-developed quartz solubility diagrams and CL-aided fluid inclusion microthermometry, it is proposed that precipitation of the earliest quartz (Qz1) was controlled by CO2 content increase and subordinately affected by decompressional cooling, leading to the formation of the early thick gold-barren veins (V1); the second generation of quartz (Qz2a) was formed by the same fluids that may have been diluted and cooled by meteoric water, leading to a greatly reduced quantity of quartz and the deposition of pyrite and gold; and the third generation of quartz (Qz2b) was deposited along with polymetallic sulfides, due to fluid cooling following a quartz dissolution event likely induced by cooling in retrograde solubility region and/or CO2 content decrease. This research may elucidate gold formation processes in orogenic intrusion—related deposits, and points to imperative CL-based in situ analyses for future studies.
A Molecular Candle Where Few Molecules Shine: HeHHe+
Authors: Fortenberry Ryan C. , Wiesenfeld Laurent
Published: May 2020

HeHHe + is the only potential molecule comprised of atoms present in the early universe that is also easily observable in the infrared. This molecule has been known to exist in mass spectrometry experiments for nearly half-a-century and is likely present, but as-of-yet unconfirmed, in cold plasmas. There can exist only a handful of plausible primordial molecules in the epochs before metals (elements with nuclei heavier than 4 He as astronomers call them) were synthesized in the universe, and most of these are both rotationally and vibrationally dark. The current work brings HeHHe + into the discussion as a possible (and potentially only) molecular candle for probing high-z and any metal-deprived regions due to its exceptionally bright infrared feature previously predicted to lie at 7.43 μ m. Furthermore, the present study provides new insights into its possible formation mechanisms as well as marked stability, along with the decisive role of anharmonic zero-point energies. A new entrance pathway is proposed through the triplet state ( 3 B 1 ) of the He 2 H + molecule complexed with a hydrogen atom and a subsequent 10.90 eV charge transfer/photon emission into the linear and vibrationally-bright 1 Σ g + HeHHe + form.
Walnut (Juglans regia L.) Septum: Assessment of Bioactive Molecules and In Vitro Biological Effects
Authors: Rusu Marius Emil , Fizesan Ionel , Pop Anca , Mocan Andrei , Gheldiu Ana-Maria , Babota Mihai , Vodnar Dan Cristian , Jurj Ancuta , Berindan-Neagoe Ioana , Vlase Laurian , Popa Daniela-Saveta
Published: May 2020

Walnut (Juglans regia L.) septum represents an interesting bioactive compound source by-product. In our study, a rich phenolic walnut septum extract, previously selected, was further examined. The tocopherol content determined by liquid chromatography-tandem mass spectrometry (LC-MS/MS) revealed higher amounts of α-tocopherol compared to γ- and δ-tocopherols. Moreover, several biological activities were investigated. The in vitro inhibiting assessment against acetylcholinesterase, α-glucosidase, or lipase attested a real management potential in diabetes or obesity. The extract demonstrated very strong antimicrobial potential against Staphylococcus aureus, Pseudomonas aeruginosa and Salmonella enteritidis. It also revealed moderate (36.08%) and strong (43.27%) antimutagenic inhibitory effects against TA 98 and TA 100 strains. The cytotoxicity of the extract was assessed on cancerous (A549, T47D-KBluc, MCF-7) and normal (human gingival fibroblasts (HGF)) cell lines. Flow cytometry measurements confirmed the cytotoxicity of the extract in the cancerous cell lines. Additionally, the extract demonstrated antioxidant activity on all four cell types, as well as anti-inflammatory activity by lowering the inflammatory cytokines (interleukin-6 (IL-6), interleukin-8 (IL-8), interleukin-1 β (IL-1β)) evaluated in HGF cells. To the best of our knowledge, most of the cellular model analyses were performed for the first time in this matrix. The results prove that walnut septum may be a potential phytochemical source for pharmaceutical and food industry.
Synthesis and Facile Dearomatization of Highly Electrophilic Nitroisoxazolo[4,3-b]pyridines
Authors: Bastrakov Maxim A. , Fedorenko Alexey K. , Starosotnikov Alexey M. , Fedyanin Ivan V. , Kokorekin Vladimir A.
Published: May 2020

A number of novel 6-R-isoxazolo[4,3-b]pyridines were synthesized and their reactions with neutral C-nucleophiles (1,3-dicarbonyl compounds, π-excessive (het)arenes, dienes) were studied. The reaction rate was found to be dependent on the nature of the substituent 6-R. The most reactive 6-nitroisoxazolo[4,3-b]pyridines are able to add C-nucleophiles in the absence of a base under mild conditions. In addition, these compounds readily undergo [4+2]-cycloaddition reactions on aromatic bonds C=C(NO2) of the pyridine ring, thus indicating the superelectrophilic nature of 6-NO2-isoxazolo[4,3-b]pyridines.
Combined Machine Learning and Molecular Modelling Workflow for the Recognition of Potentially Novel Fungicides
Authors: Jović Ozren , Šmuc Tomislav
Published: May 2020

Novel machine learning and molecular modelling filtering procedures for drug repurposing have been carried out for the recognition of the novel fungicide targets of Cyp51 and Erg2. Classification and regression approaches on molecular descriptors have been performed using stepwise multilinear regression (FS-MLR), uninformative-variable elimination partial-least square regression, and a non-linear method called Forward Stepwise Limited Correlation Random Forest (FS-LM-RF). Altogether, 112 prediction models from two different approaches have been built for the descriptor recognition of fungicide hit compounds. Aiming at the fungal targets of sterol biosynthesis in membranes, antifungal hit compounds have been selected for docking experiments from the Drugbank database using the Autodock4 molecular docking program. The results were verified by Gold Protein-Ligand Docking Software. The best-docked conformation, for each high-scored ligand considered, was submitted to quantum mechanics/molecular mechanics (QM/MM) gradient optimization with final single point calculations taking into account both the basis set superposition error and thermal corrections (with frequency calculations). Finally, seven Drugbank lead compounds were selected based on their high QM/MM scores for the Cyp51 target, and three were selected for the Erg2 target. These lead compounds could be recommended for further in vitro studies.
Investigation of the Dynamism of Nanosized SOA Particle Formation in Indoor Air by a Scanning Mobility Particle Sizer and Proton-Transfer-Reaction Mass Spectrometry
Authors: Pytel Klaudia , Marcinkowska Renata , Zabiegała Bożena
Published: May 2020

Terpenes are VOCs of particular importance, since they are emitted from a wide range of indoor sources and are considered to be precursors of Secondary Organic Aerosol (SOA) formation. It has been proven that SOA particles, especially nanosized ones, pose a threat to human health. In this research, experiments with the application of an environmental chamber and real-time measurement techniques were carried out to investigate in a complimentary way the formation of monoterpene oxidation products and nanosized SOA particles initiated by monoterpene ozonolysis. Proton-Transfer-Reaction Mass Spectrometry with a Time-Of-Flight analyzer (PTR-TOF-MS) and a Scanning Mobility Particle Sizer (SMPS) were applied to determine in real time the dynamism of the formation of the corresponding terpene ozonolysis products and submicron SOA particles. Results proved that firstly, oxidation products were formed, and then, they underwent nucleation and condensation, forming particles whose diameters grew with time. The oxidation products formed were different depending on the type of terpenes applied. The comparison of the results obtained during the experiments with gaseous standard mixtures and real samples commonly present and used in indoor air revealed that the diversified chemical composition of the emission source had implications for both the particle formation initiated by the oxidation of essential oil components and the chemical reactions occurring via the oxidation process. With the instrumentation utilized, the concentration changes at the level of a few ppbv could be monitored.
Multistep Photochemical Reactions of Polypyridine-Based Ruthenium Nitrosyl Complexes in Dimethylsulfoxide
Authors: Marchenko Nataliia , Lacroix Pascal G. , Bukhanko Valerii , Tassé Marine , Duhayon Carine , Boggio-Pasqua Martial , Malfant Isabelle
Published: May 2020

The photorelease of nitric oxide (NO·) has been investigated in dimethylsulfoxide (DMSO) on two compounds of formula [Ru(R-tpy)(bpy)(NO)](PF6)3, in which bpy stands for 2,2′-bipyridine and R-tpy for the 4′-R-2,2′:6′,2″-terpyridine with R = H and MeOPh. It is observed that both complexes are extremely sensitive to traces of water, leading to an equilibrium between [Ru(NO)] and [Ru(NO2)]. The photoproducts of formula [Ru(R-tpy)(bpy)(DMSO)](PF6)2 are further subjected to a photoreaction leading to a reversible linkage isomerization between the stable Ru-DMSO(S) (sulfur linked) and the metastable Ru-DMSO(O) (oxygen linked) species. A set of 4 [Ru(R-tpy)(bpy)(DMSO)]2+ complexes (R = H, MeOPh, BrPh, NO2Ph) is investigated to characterize the ratio and mechanism of the isomerization which is tentatively related to the difference in absorbance between the Ru-DMSO(S) and Ru-DMSO(O) forms. In addition, the X-ray crystal structures of [Ru(tpy)(bpy)(NO)](PF6)3 and [Ru(MeOPh-tpy)(bpy)(DMSO(S))](PF6)2 are presented.
Hypergolics in Carbon Nanomaterials Synthesis: New Paradigms and Perspectives
Authors: Chalmpes Nikolaos , Spyrou Konstantinos , Vasilopoulos Konstantinos C. , Bourlinos Athanasios B. , Moschovas Dimitrios , Avgeropoulos Apostolos , Gioti Christina , Karakassides Michael A. , Gournis Dimitrios
Published: May 2020

Recently we have highlighted the importance of hypergolic reactions in carbon materials synthesis. In an effort to expand this topic with additional new paradigms, herein we present novel preparations of carbon nanomaterials, such-like carbon nanosheets and fullerols (hydroxylated fullerenes), through spontaneous ignition of coffee-sodium peroxide (Na2O2) and C60-Na2O2 hypergolic mixtures, respectively. In these cases, coffee and fullerenes played the role of the combustible fuel, whereas sodium peroxide the role of the strong oxidizer (e.g., source of highly concentrated H2O2). The involved reactions are both thermodynamically and kinetically favoured, thus allowing rapid product formation at ambient conditions. In addition, we provide tips on how to exploit the released energy of such highly exothermic reactions in the generation of useful work.