bluuebunny/crossref_metadata_2025 huggingface dataset:
Isolation and characterization of pigeon breast muscle cytosolic 5'-nucleotidase-I (cN-I).
Authors: Tkacz-Stachowska Kinga , Lechward Katarzyna , Skladanowski Andrzej C
Published: November 2019

5'-Nucleotidase specific towards dCMP and AMP was isolated from avian breast muscle and characterized. It was found to be similar to a type-I form (cN-I) identified earlier as the AMP-selective 5'-nucleotidase responsible for adenosine formation during ATP breakdown in transfected COS-7 cells. Expression pattern of the cN-I gene in pigeon tissues indicated breast muscle as a rich source of the transcript. We purified the enzyme from this source using two-step chromatography and obtained an active homogenous preparation, free of ecto-5'-nucleotidase activity. The tissue content of the activity was calculated at 0.09 U/g wet weight. The specific activity of the enzyme preparation was 4.33 U/mg protein and it preferred dCMP and AMP to dAMP and IMP as a substrate. Its kinetic properties were very similar to those of the enzyme purified earlier from heart tissue. It was strongly activated by ADP. Inhibition by inorganic phosphate was more pronounced than in heart-isolated cN-I. Despite this difference, a similar physiological function is suggested for cN-I in both types of muscle.
The role of benzoate secreted by Desulfotomaculum acetoxidans DSM 771 in sulfate uptake.
Authors: Pawłowska-Cwiek Lucyna , Pado Ryszard
Published: November 2019

This work was designed to find the cause of the delay in hydrogen sulfide dissimilation in Desulfotomaculum acetoxidans DSM 771, which is dependent on the sulfate uptake. This bacterium grown without addition of any aromatic compound was shown by spectrum analysis with the methylene method to contain hydroxy-benzoate derivatives. The presence of these compounds was confirmed by HPLC in fractions obtained from cell walls after 15 days of culture. The test with 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt seemed to indicate the presence of peroxidase, which probably oxidized benzoate to its hydroxy derivatives. The test with 5-sulfo-salicylic acid proved the ability of the investigated strain to utilize arylsulfates and to reduce sulfate group to hydrogen sulfide. On the basis of the above data, we propose the following sequence of reactions: 1, benzoate secretion; 2, benzoate hydroxylation; 3, sulfonation of hydroxy-benzoate derivatives.
The role of Na+/H+ exchanger in serotonin secretion from porcine blood platelets.
Authors: Tomasiak Marian , Ciborowski Michal , Stelmach Halina
Published: November 2019

This study was undertaken to evaluate whether a link exists between the activation of protein kinase C (PKC), operation of Na(+)/H(+) exchanger (NHE), cell swelling and serotonin (5-HT) secretion in porcine platelets. Activation of platelets by thrombin or phorbol 12-myristate 13-acetate (PMA), a PKC activator, initiated a rapid rise in the activity of Na(+)/H(+) exchanger and secretion of 5-HT. Both thrombin- and PMA-evoked activation of Na(+)/H(+) exchanger was less pronounced in the presence of ethyl-isopropyl-amiloride (EIPA), an NHE inhibitor, and by GF 109203X, a PKC inhibitor. Monensin (simulating the action of NHE) caused a dose-dependent release of 5-HT that was not abolished by GF 109203X or EGTA. Lack of Na(+) in the suspending medium reduced thrombin-, PMA-, and monensin-evoked 5-HT secretion. GF 109203X nearly completely inhibited 5-HT release induced by PMA-, partly that induced by thrombin, and had no effect on 5-HT release induced by monensin. EIPA partly inhibited 5-HT release induced by thrombin and nearly totally that evoked by PMA. Electronic cell sizing measurements showed an increase in mean platelet volume upon treatment of cells with monensin, PMA or thrombin. The PMA- and thrombin-evoked rise in mean platelet volume was strongly reduced in the presence of EIPA. As judged by optical swelling assay monensin and PMA produced a rapid rise in platelet volume. The swelling elicited by PMA was inhibited by EIPA and its kinetics was similar to that observed in the presence of monensin. Hypoosmotically evoked platelet swelling did not affect platelet aggregation but significantly potentiated thrombin-evoked release of 5-HT and ATP. Taken together, these results show that in porcine platelets PKC may promote 5-HT secretion through the activation of NHE. It is hypothesized that enhanced Na(+)/H(+) antiport may result in a rise in cell membrane tension (due to cell swelling) which in turn facilitates fusion of secretory granules with the plasma membrane leading to 5-HT secretion.
The HPV16 E2 transcriptional regulator mode of action depends on the physical state of the viral genome.
Authors: Schmidt Marcin T , Olejnik Agnieszka K , Goździcka-Józefiak Anna
Published: November 2019

Human papillomavirus (HPV) infection is a major risk factor for the development of cervical cancer. The HPV-induced immortalization of epithelial cell usually requires integration of the viral DNA into the host cell genome. The integration event causes disruption of the E2 gene and this is followed by overexpression of the E6 and E7 oncoproteins. The E2 protein is a transcription factor that regulates expression of the E6 and E7 oncoproteins by binding to four sites within the viral long control region. We used an in vitro cell culture model to explore the role of the E2 protein in the transcriptional control of the HPV16 long control region. Employing transient and stable transfection experiments we simulated the episomal and integrated states of the viral genome, respectively. We show that the E2 protein up-regulates E6/E7 transcription from episomal DNA but represses it in the case of integrated DNA. The activator function of the E2 protein seems to counteract the repressive chromatin structure formed over episomal DNA. Steroid hormones and retinol also modulate oncogene transcription differently depending on the physical structure of the viral DNA. Our data suggest regulatory mechanisms involving interactions between the E2 protein and nuclear hormone receptors.
Two types of non-homologous RNA recombination in brome mosaic virus.
Authors: Alejska Magdalena , Malinowska Nelli , Urbanowicz Anna , Figlerowicz Marek
Published: November 2019

Non-homologous RNA recombination is a process enabling the exchange of genetic material between various (related or unrelated) RNA-based viruses. Despite extensive investigations its molecular mechanism remains unclear. Studies on genetic recombination in brome mosaic virus (BMV) have shown that local hybridization between genomic RNAs induces frequent non-homologous crossovers. A detailed analysis of recombinant structures suggested that local complementary regions might be involved in two types of non-homologous recombination in BMV: site-specific and heteroduplex-mediated. To verify the above hypothesis and better recognize the mechanism of the phenomenon studied we have tested how the putative types of recombination are affected by a specific mutation in the BMV polymerase gene or by changes in RNA structure. The experiments undertaken revealed substantial differences between site-specific and heteroduplex-mediated recombination, indicating that they occur according to different mechanisms. The former can be classified as homology-assisted, and the latter as homology-independent. In addition to local RNA/RNA hybridization, short regions of homology are required for site-specific crossovers to occur. They are most efficiently mediated if one homologous sequence is located at the beginning of and the second just before a double-stranded region. At present it is difficult to state what is the mechanism of heteroduplex-mediated recombination. Earlier it was postulated that strong RNA/RNA interaction enforces template switching by the viral replicase. There are, however, several observations questioning this model and indicating that some other factors, which are still unknown, may influence heteroduplex-mediated crossovers.
Influence of reagent formulation on mRNA quantification by RT-PCR using imported external standard curves.
Authors: Farriol Mireia , Orta Xavi
Published: November 2019

Use of an imported external standard curve is common in real-time quantitative RT-PCR. Two practical strategies for long-term experiments include importing a grand mean standard curve to all accumulated runs or using daily imported standard curves, fixing the slope at the beginning of the experiment and calibrating successive runs with curves generated from this imported slope, adding a single standard that registers the variation in the y-intercept. This study determines the influence that a change in reagent lots has on these two calibration approaches when determining mRNA copy numbers of the ornithine decarboxylase and porphobilinogen deaminase genes. Two sets of determinations were run with the use of lot A and lot B. A marked decrease in the crossing points (Cp) in the standards for both genes at all concentration levels was observed with the change in lots. A grand mean standard curve was generated for each gene and each set and comparisons between the sets were performed. Statistically significant differences were found with respect to the y-intercept but not the slope, suggesting that the change of reagent lot affected the detection sensitivity but not the efficiency of the reaction. The excellent correlation coefficients obtained for these curves for each gene were not achieved when overall data from both sets were combined to generate an overall grand mean standard curve. We conclude that when faced with a change of RT-PCR reagent lot that will affect the detection sensitivity of the method, samples should be calculated with either the daily imported standard curves or with the respective grand mean standard curve for each lot.
Ribosomal proteins L34 and S29 of amphioxus Branchiostoma belcheri tsingtauense: cDNAs cloning and gene copy number.
Authors: Liu Lina , Zhang Shicui , Liu Zhenhui , Li Hongyan , Liu Mei , Wang Yongjun , Ma Lifang
Published: November 2019

The complete cDNA and deduced amino-acid sequences of ribosomal proteins L34 (AmphiL34) and S29 (AmphiS29) from the amphioxus Branchiostoma belcheri tsingtauense were identified in this study. The AmphiL34 cDNA is 435 nucleotides in length and encodes a 118 amino-acid protein with calculated molecular mass of 13.6 kDa. It shares 53.6-67.5% amino-acid sequence identity with its eukaryotic counterparts including human, mouse, rat, pig, frog, catfish, fruit fly, mosquito, armyworm, nematode and yeast. The AmphiS29 cDNA comprises 453 nucleotides and codes for a 56 amino-acid protein with a calculated molecular mass of 6.6 kDa. It shows 66.1-78.6% amino-acid sequence identity to eukaryotic S29 proteins from human, mouse, rat, pig, zebrafish, seahorse, fruit fly, nematode, sea hare and yeast. AmphiL34 contains a putative nucleolar localization signal, while AmphiS29 has a zinc finger-like domain. A phylogenetic tree deduced from the conserved sequences of AmphiL34 and AmphiS29 and other known counterparts indicates that the positions of AmphiL34/AmphiS29 are intermediate between the vertebrate and invertebrate L34/S29. Southern blot analysis demonstrates the presence of one copy of the L34 gene and 2-3 copies of the S29 gene in the genome of the amphioxus B. belcheri tsingtauense. This is in sharp contrast to the existence of 7-9 copies of the L34 gene and 14-17 copies of the S29 gene in the rat genome. These date suggest that housekeeping genes like AmphiL34 and AmphiS29 have undergone large-scale duplication in the chordate lineage.
Characterization of a novel protein that specifically binds to DNA modified by N-acetoxy-acetylaminofluorene and cis-diamminedichloroplatinum.
Authors: Pietrowska Monika , Widłak Piotr
Published: November 2019

Proteins recognizing DNA damaged by the chemical carcinogen N-acetoxy-acetylaminofluorene (AAAF) were analyzed in nuclear extracts from rat tissues, using a 36 bp oligonucleotide as a substrate and electrophoretic mobility shift and Southwestern blot assays. One major damage-recognizing protein was detected, whose amount was estimated as at least 10(5) copies per cell. Levels of this protein were similar in extracts from brain, kidney and liver, but much lower in extracts from testis. The affinity of the detected protein for DNA damaged by AAAF was about 70-fold higher than for undamaged DNA. DNA damaged by cis-diamminedichloroplatinum (cis-DDP), benzo(a)pyrene diolepoxide (BPDE) or UV-radiation also bound this protein with an increased affinity, the former more strongly and the latter two more weakly as compared to AAAF-damaged DNA. The detected AAAF/DDP-damaged-DNA-binding (AAAF/DDP-DDB) protein had a molecular mass of about 25 kDa and was distinct from histone H1 or HMGB proteins, which are known to have a high affinity for cis-DDP-damaged DNA. The level of this damage-recognizing protein was not affected in rats treated with the carcinogen 2-acetylaminofluorene. The activity of an AAAF/DDP-DDB protein could also be detected in extracts from mouse liver cells but not from the Hep2G human hepatocellular carcinoma.
Kinobeon A, purified from cultured safflower cells, is a novel and potent singlet oxygen quencher.
Authors: Kambayashi Yasuhiro , Takekoshi Susumu , Nakano Minoru , Shibamori Masafumi , Hitomi Yoshiaki , Ogino Keiki
Published: November 2019

We recently reported that kinobeon A, produced from safflower cells, suppressed the free radical-induced damage of cell and microsomal membranes. In the present study, we investigated whether kinobeon A quenches singlet oxygen, another important active oxygen species. Kinobeon A inhibited the singlet oxygen-induced oxidation of squalene. The second-order rate constant between singlet oxygen and kinobeon A was 1.15 x 10(10) M(-1)s(-1) in methanol containing 10% dimethyl sulfoxide at 37 degrees C. Those of alpha-tocopherol and beta-carotene, which are known potent singlet oxygen quenchers, were 4.45 x 10(8) M(-1)s(-1) and 1.26 x 10(10) M(-1)s(-1), respectively. When kinobeon A was incubated with a thermolytic singlet oxygen generator, its concentration decreased. However, this change was extremely small compared to the amount of singlet oxygen formed and the inhibitory effect of kinobeon A on squalene oxidation by singlet oxygen. In conclusion, kinobeon A was a strong singlet oxygen quencher. It reacted chemically with singlet oxygen, but it was physical quenching that was mainly responsible for the elimination of singlet oxygen by kinobeon A. Kinobeon A is expected to have a preventive effect on singlet oxygen-related diseases of the skin or eyes.
Modulation of ERK1/2 activity is crucial for sphingosine-induced death of glioma C6 cells.
Authors: Krzemiński Patryk
Published: November 2019

In this study the contribution of the ERK1/2 pathway to sphingosine-induced death and morphological changes of the actin cytoskeleton in glioma C6 cells was investigated. Surprisingly, the level of ERK1/2 phosphorylation does not change after incubation of cells with sphingosine. Despite this, sphingosine induces rounding and detachment of cells without formation of apoptotic bodies. To shed light on this process, a specific inhibitor of ERK1/2 phosphorylation, U0126, was used. Cells incubated simultaneously with sphingosine and U0126 not only detached, but also exhibited formation of apoptotic-like blebs. These data suggest that during sphingosine-induced glioma C6 cell death apoptotic blebbing is dependent on ERK1/2 signalling and occurs only when ERK1/2 activity is decreased or abolished.
Does in vitro replicative senescence of human CD8+ cells reflect the phenotypic changes observed during in vivo ageing?
Authors: Brzezinska Agnieszka
Published: November 2019

Immunosenescence is viewed as a remodeling process with the exhaustion of naïve T cells and filling up of the immunological space with memory cells. In this study some phenotypic changes of CD8(+) human cells during in vivo ageing were compared with those observed in long term cultures of lymphocytes derived from cord blood or from peripheral blood from donors of different age. Both in vivo and in vitro a significant decrease of the fraction of CD8(+)CD28(+) cells was observed. Comparing the proportions of other T cell subpopulations (the CD4/CD8(+) ratio, CD56, CD57, CD27) made it possible to conclude that replicative senescence in vitro partially reflects in vivo ageing.
Congo red interaction with alpha-proteins.
Authors: Sereikaite Jolanta , Bumelis Vladas-Algirdas
Published: November 2019

The ability of Congo red to form complexes with alpha-proteins, human growth hormone and human interferon-alpha2b, was found by absorption difference spectroscopy. A human growth hormone-Congo red complex was isolated by gel-permeation chromatography, and its visible absorption spectrum was registered in comparison to free dye. The ability of Congo red to induce dimerization of human growth hormone was demonstrated using chemical cross-linking agents 1,3,5-triacryloyl-hexahydro-s-triazine and ethylene glycol bis(succinimidylsuccinate).
Interaction of anthracene and its oxidative derivatives with human serum albumin.
Authors: Skupińska Katarzyna , Zylm Monika , Misiewicz Irena , Kasprzycka-Guttman Teresa
Published: November 2019

Binding affinities of ten polycyclic aromatic hydrocarbons to albumin were determined: anthracene, its eight oxy-derivatives: anthraquinone, 9-anthracenemethanol, 9-anthraldehyde, 9-anthracenecarboxylic acid, 1,4-dihydroxyanthraquinone, 1,5-dihydroxyanthraquinone, 1,8-dihydroxyanthraquinone, 2,6-dihydroxyanthraquinone and benzo[a]pyrene. The quenching of albumin fluorescence was used to measure the PAH - protein interaction. The theoretical curve of calculated fluorescence was fitted to experimental data after necessary corrections regarding PAHs fluorescence and inner filter effect. From the numerical fitting the final association constants were calculated. Anthracene and anthraquinone failed to quench the albumin fluorescence. 9-anthracenecarboxylic acid showed the highest, while 9-anthracenemethanol the weakest albumin binding affinity. The affinity constants determined for 9-anthraldehyde and benzo[a]pyrene were of the same magnitude and indicated low-affinity binding to albumin. The constants obtained for the four dihydroxyanthraquinones were higher, but dissimilar, which suggests that the position of the functional group in anthracene molecule influences the binding constant. Moreover, this study suggests that the type of substituent plays a significant role in PAH-albumin complex formation. The carboxylic group increases the binding affinity of the anthracene molecule the most rather than the presence of both carbonyl and hydroxyl groups. The lowest affinity constants were obtained for aldehyde, methyl and carbonyl substituents.
The influence of modification at position 2 on the side-chain conformation in oxytocin analogs.
Authors: Smiech Bogusław , Kaźmierkiewicz Rajmund , Lammek Bernard
Published: November 2019

The nonapeptide oxytocin (OT) is used in medicine to help begin and/or continue childbirth. Its analogs can be also used to control bleeding following fetus delivery. The main function of oxytocin is to stimulate contraction of uterus smooth muscle and the smooth muscle of mammary glands, thus regulating lactation. This paper describes theoretical simulations of the distribution of the torsional angles chi1 in the non-standard methylated phenylalanine residues of three oxytocin analogs: [(Phe)(2)o-Me]OT, [(Phe)(2)m-Me]OT, [(Phe)(2)p-Me]OT. The conformations of the oxytocin analogs were studied both in vacuum and in solution. We found some correlations between the biological activity of the considered peptides and the side-chain conformations of amino-acid residues 2 and 8.
Altered mouse leukemia L1210 thymidylate synthase, associated with cell resistance to 5-fluoro-dUrd, is not mutated but rather reflects posttranslational modification.
Authors: Cieśla Joanna , Fraczyk Tomasz , Zieliński Zbigniew , Sikora Jacek , Rode Wojciech
Published: November 2019

Thymidylate synthase purified from 5-fluoro-dUrd-resistant mouse leukemia L1210 cells (TSr) was less sensitive to slow-binding inhibition by 5-fluoro-dUMP than the enzyme from the parental cells (TSp), both enzyme forms differing also in sensitivity to several other dump analogues, apparent molecular weights of monomer and dimer, and temperature dependence of the catalyzed reaction. Direct sequencing of products obtained from RT-PCR, performed on total RNA isolated from the parental and 5-fluoro-dUrd-resistant cells, proved both nucleotide sequences to be identical to the mouse thymidylate synthase coding sequence published earlier (NCBI protein database access no. NP_067263). This suggests that the altered properties of TSr are caused by a factor different than protein mutation, presumably posttranslational modification. As a possibility of rat thymidylate synthase phosphorylation has been recently demonstrated (Samsonoff et al. (1997) J Biol Chem 272: 13281), the mouse enzyme amino-acid sequence was analysed, revealing several potential phosphorylation sites. In order to test possible influence of the protein phosphorylation state on enzymatic properties, endogenous TSp and TSr were purified in the presence of inhibitors of phosphatases. Although both enzyme forms were phosphorylated, as shown by electrophoretical separation followed by phosphoprotein detection, the extent of phosphorylation was apparently similar. However, the same two purified enzyme preparations, compared to the corresponding preparations purified in the absence of phosphatase inhibitors, showed certain properties, including sensitivity to the slow-binding inhibition by FdUMP, altered. Thus properties dependence on phosphorylation was indicated.
Association of model peptides and dehydropeptides: N-acetyl-L-butyrine and (Z)-dehydrobutyrine N',N'-dimethylamides.
Authors: Broda Małgorzata A , Rzeszotarska Barbara
Published: November 2019

These comparative studies on the aggregation behaviour of Ac-(Z)-DeltaAbu-NMe(2) and Ac-L-Abu-NMe(2) in carbon tetrachloride were performed by the analysis of their FTIR spectra and by theoretical calculations. The percentage of the monomeric form (alpha) decreased as concentration increased and this occurred to a higher degree for the (Z)-DeltaAbu derivative than for its saturated analogue. The dimerization constant K(D), calculated on the basis of the intensity of the monomer and associate bands in the nu(s)(N-H) vibration region, is by three orders of magnitude larger for Ac-(Z)-DeltaAbu-NMe(2) than for Ac-L-Abu-NMe(2). The obtained dimer geometries of the dehydro- compound were calculated by the B3LYP/6-31+G** method.
Glutathione and GSH-dependent enzymes in patients with liver cirrhosis and hepatocellular carcinoma.
Authors: Czeczot Hanna , Scibior Dorota , Skrzycki Michał , Podsiad Małgorzata
Published: November 2019

We investigated glutathione level, activities of selenium independent GSH peroxidase, selenium dependent GSH peroxidase, GSH S-transferase, GSH reductase and the rate of lipid peroxidation expressed as the level of malondialdehyde in liver tissues obtained from patients diagnosed with cirrhosis or hepatocellular carcinoma. GSH level was found to be lower in malignant tissues compared to adjacent normal tissues and it was higher in cancer than in cirrhotic tissue. Non-Se-GSH-Px activity was lower in cancer tissue compared with adjacent normal liver or cirrhotic tissue, while Se-GSH-Px activity in cancer was found to be similar to its activity in cirrhotic tissue and lower compared to control tissue. An increase in GST activity was observed in cirrhotic tissue compared with cancer tissue, whereas the GST activity in cancer was lower than in adjacent normal tissue. The activity of GSH-R was similar in cirrhotic and cancer tissues, but higher in cancer tissue compared to control liver tissue. An increased level of MDA was found in cancer tissue in comparison with control tissue, besides its level was higher in cancer tissue than in cirrhotic tissue. Our results show that the antioxidant system of cirrhosis and hepatocellular carcinoma is severely impaired. This is associated with changes of glutathione level and activities of GSH-dependent enzymes in liver tissue. GSH and enzymes cooperating with it are important factors in the process of liver diseases development.
Rozwój współpracy w zakresie oceny technologii medycznych w krajach Unii Europejskiej
Authors: Baran-Kooiker Aleksandra , Czech Marcin , Hołownia-Voloskova Malwina
Published: November 2019

The purpose of the note below is to familiarize readers with the current status and future initiatives of cooperation in the field of health technology assessment (HTA) in the European Union (EU). The European Commission is trying to create a framework for efficient collaboration between member states of EU in the field of HTA and is gradually working on strengthening it. The following initiatives, projects and programs have already been implemented:

-  Project on Coordination and Development of Health Care Technology Assessment in Europe

-  The European Collaboration for Assessment of Health Interventions and Technology

-  European Network for Health Technology Assessment 

-  Beneluxa Initiative, The Valletta Declaration, FINOSE and Fair and Affordable Pricing

-  European Network for Health Technology Assessment

Currently there is an ongoing proposal for an HTA Regulation of the European Parliament and the Council, which is the measurable result of this cooperation. A key element of the proposed regulation is a centralized joint clinical assessment (for HTA purposes) of the efficacy of the medicinal product/medical device.
PROACTIVE WORK BEHAVIOR AND INNOVATIVE WORK BEHAVIOR: MODERATING EFFECT OF JOB CHARACTERISTICS
Authors: Nurjaman Kadar , Sandi Marta M , Eliyana Anis , Kurniasari Dewi , Kurniasari Dedeh
Published: November 2019

Purpose of study: This study examined the usefulness of job characteristics as a technique designed to enhance the effect of proactive behavior and innovate work behavior. Methodology: A field study was conducted with a randomly selected sample of company 145 supervisors of Sharia Bank at West Java Indonesia. This research was used as a quantitative approach to analyze and answer all research questions. And then, hypotheses for direct and moderator effects are tested using hierarchical regression analysis with SPSS as an analysis tool. Result: Results presented that there is a relation between proactive behavior and innovative work behavior. Furthermore, jobs characteristic strengthens the relationship between proactive behavior and innovative work behavior in Sharia Bank. Implications: The strategy of job design utilization with job characteristics can trigger people’s innovation behavior who already have self-initiative and this strategy will succeed in Islamic banks that have employees with high initiative. Novelty/originality: The novelty of this study is the use of job characteristics in strengthening the relationship between proactive behavior and IWB which is very rarely done in banking, especially in Sharia Banking.
INDIGENOUS TRADITION BASED-TOURISM DEVELOPMENT: FOREIGN TOURIST’S MEMORABLE TOURISM EXPERIENCE IN MENTAWAI, INDONESIA
Authors: Elfiondri  , Zaitul  , Pratimaratri Uning , Kartika Diana , Amril Oslan
Published: November 2019

Purpose: This study examines the foreign tourist’s MTE on the indigenous tradition with the objective to find out the foreign tourists’ MTE and its effect on revisit intention and intention to recommend. It is conducted on 51 foreign tourists visiting Mentawai from April to June 2018.

Methodology: Convenient sampling is used for the sampling method. Primary data are collected from survey.

Result: The result shows that MTE is good (higher) which has a positive significant relationship with the revisit intention due to that the t value of this variable is greater than 1.96., and the path coefficient is 0.619. It indicates the higher MTE which will have an effect on the high opportunity to revisit intention. The effect of MTE on intention to recommend also has a positive significant relationship. The path coefficient also indicates a positive sign, meaning the higher MTE has effect on the higher intention to recommend. Thus, foreign tourists experiencing Mentawai cultural tourism has positive MTE effecting positively tourists’ revisit intention and intention to recommend.

Applications: This research can be used for universities, teachers, and students.

Novelty/Originality: In this research, the model of the Indigenous Tradition Based-Tourism Development: Foreign Tourist’s Memorable Tourism Experience in Mentawai, Indonesia is presented in a comprehensive and complete manner.
THE RELATIONSHIP OF RISK MANAGEMENT AND BANK PROFITABILITY PERFORMANCE BETWEEN DOMESTIC AND FOREIGN ISLAMIC BANKS IN MALAYSIA
Authors: S. W. Chong Venny , M. S. Lam Jason , H.Tan S.
Published: November 2019

Purpose: This study is to determine the effects of risk management towards the domestic and foreign Islamic bank’s financial performance in Malaysia. The ten Islamic banks in Malaysia have been chosen as the sample bank in which domestic and foreign banks were equally divided. The credit risk, liquidity risk as well as solvency risk acted as the independent variables to determine the effects towards the bank’s profitability as measured by return on equity.

Methodology: The panel data analysis has employed fixed effect and random effect regression models and the Hausman test in this study. Furthermore, the independent sample T-test was conducted to examine the significant difference between domestic and foreign Islamic banks.

Result: The finding of this study showed that liquidity risk and insolvency risk would have a greater impact towards the Islamic bank’s profitability while the credit risk has no significant influence on Islamic bank’s financial performance in Malaysia. The study concludes that domestic Islamic banks had better financial performance as compared to foreign Islamic banks in Malaysia.

Applications: This research can be used for universities, teachers, and students.

Novelty/Originality: In this research, the model of The Relationship of Risk Management and Bank Profitability Performance between Domestic and Foreign Islamic Banks in Malaysia is presented in a comprehensive and complete manner.
Developing a Reflection Guiding Tool for underperforming medical students: An action research project
Authors: Holder Nurul Atira Khairul Anhar , Sim Zhi Liang , Foong Chan Choong , Pallath Vinod
Published: November 2019

The ability to reflect is an important generic competence especially for underperforming students as they have to analyse their previous learning experiences to improve on future academic performances. This action research describes the process of a team when developing a reflection guiding tool (RGT). As the underperforming students may have difficulty in comprehending what is expected from them while using a reflective approach, we designed the RGT based on the six steps in Gibbs cycle; 1) Description, 2) Feelings/Reactions, 3) Evaluation, 4) Analysis, 5) Conclusions, and 6) Personal action plan. These underperforming students reflected on how, and why, they failed their assessments based on the proposed RGT. Findings revealed that RGT was able to make students aware of what reflection skill is and thus fulfilled its objective. It helped Year 1 and Year 2 underperforming medical students to reflect on their academic failure.Received: 05 September 2019Accepted: 22 November 2019Published online: 29 November 2019
The individual watching of one’s own video and its influence on future biology teachers´ professional vision
Authors: Němečková Linda , Pavlasová Lenka
Published: November 2019

The student teacher usually learns their trade under the guidance of a mentor during teaching practice. Reflections usually take place after the mentor’s observation of the student´s lesson, and take the form of an interview between the two participants. Recently, video recordings have been used advantageously to add an extra dimension to such professional development. In the classroom, at the time of the lesson, future teachers may not notice certain events, but using video helps them review, and analyse their attributes, thereby making the reflective process more effective. The development of ‘professional vision’ consists of two processes: (1) noticing different events and (2) assessing and appraising themselves.1 This study analysed four written reflections (two pairs from each) from future biology teachers (n = 65) during their practice at ISCED 2 (International Standard Classification of Education 2 – lower secondary education, pupils from 11 to 15 years old) and at ISCED 3 (International Standard Classification of Education 3 - upper secondary education, pupils from 15 to 19 years old) schools. The first reflection in the pair (‘pre-reflection’) was written without video recordings, and the second (‘post-reflection’) with the support of video recordings. The reflections were divided into statements, and coded according to Sherin and van Es’ categorisation system. Statistically significant changes were found in several categories of professional vision in the students´ statements. During practice at ISCED 2 school (the first practice in the curriculum) students commented more often in the Self, Pedagogy, Management, Evaluate and Alteration categories in postreflection, and less in the Student, Curriculum, Subject, Climate and Describe categories. During (the second) practice at ISCED 3 school, students dealt more with Self and Alteration categories in post-reflection. These findings persuaded us that the use of two practices based on video-supported reflection of one’s own lessons is effective in prospective biology teachers’ development of their professional vision.Received: 28 May 2019Accepted: 08 November 2019Published online: 29 November 2019
ПОСТРОЕНИЕ АНИЗОТРОПНОЙ ГИДРОДИНАМИЧЕСКОЙ МОДЕЛИ И ИССЛЕДОВАНИЕ ВЛИЯНИЯ АНИЗОТРОПИИ ПРОНИЦАЕМОСТИ НА ПРИМЕРЕ МОДЕЛИРОВАНИЯ БЛОКА МЕСТОРОЖДЕНИЯ
Authors: Ермеков Роман Игоревич , Коровин Михаил Олегович , Меркулов Виталий Павлович , Чернова Оксана Сергеевна
Published: November 2019

Актуальность исследования определена тем, что наличие адаптированной под исторические данные эксплуатации геолого-гидродинамической модели месторождения является мощным инструментом, с помощью которого инженеры имеют возможность проводить мониторинг текущей ситуации и рассматривать эффективность предлагаемых решений в ближайшем будущем. Поэтому для получения достоверных результатов весьма важным является качество построения модели коллекторов с учётом их геологических особенностей. Одной из таких особенностей можно считать анизотропию проницаемости. Весьма важной она является при рассмотрении коллекторов с низкими фильтрационно-емкостными свойствами, к разработке которых на текущий момент активно начали приступать нефтегазодобывающие компании. В цели данной работы входила оценка влияния анизотропии проницаемости на поведение гидродинамической модели продуктивного коллектора нефтяного месторождения. Объектом исследования является одно из нефтяных месторождений Томской области, состоящее из терригенных отложений. Особенностью месторождения является его месторасположение в системе сложных локальных поднятий разного порядка. Основной продуктивный коллектор представлен юрскими отложениями, сформировавшимися в регрессивно-трансгрессивную серию осадконакопления и имеющими сложное распределение фильтрационно-емкостных свойств. Методы исследования основаны на построении модели месторождения и проведении гидродинамических расчётов, включающих подбор близкого к реальности значения анизотропии проницаемости на основе промысловых данных, оптимизацию ранее существующей системы разработки, а также построение и анализ карт разработки. В результате исследования было установлено, что упущение анизотропии проницаемости приводит к завышению накопленных показателей разработки месторождений. Было получено, что повышение величины анизотропии не всегда приводит к росту накопленной добычи нефти, что несомненно подчеркивает особенность геологического строения коллектора. На завершающем этапе был проведён гидродинамический расчёт разработки в срок на 15 лет, по результатам которого также сделаны выводы о правильности применения операций по повышению нефтеотдачи.
МАТЕМАТИЧЕСКОЕ МОДЕЛИРОВАНИЕ УПЛОТНЕНИЯ ПРОСАДОЧНЫХ ЛЁССОВЫХ ГРУНТОВ СЕВЕРНОГО КАВКАЗА ГЛУБИННЫМИ ВЗРЫВАМИ
Authors: Тарасенко Елена Олеговна , Тарасенко Василий Сергеевич , Гладков Андрей Владимирович
Published: November 2019

Актуальность. Проектирование и строительство зданий и сооружений на лёссовых просадочных грунтах на Северном Кавказе является актуальной проблемой строительства, нацеленной на обеспечение прочности и длительной эксплуатации зданий и сооружений. Актуальность проблемы связана с тем, что лёссовые грунты имеют большую мощность (до 50 м), распространены практически повсеместно (80–85 % площади) и являются основным типом грунтовых оснований во многих городах региона. Как показал анализ литературы по данной тематике, в настоящее время не существует строгого математического описания указанного процесса. Данная работа посвящена математическому моделированию уплотнения просадочных лёссовых грунтов Северного Кавказа глубинными камуфлетными взрывами. Цель: провести математическое моделирование уплотнения просадочных лёссовых грунтов Северного Кавказа глубинными взрывами. Объект: просадочные лёссовые грунты Северного Кавказа. Методы: корректное использование результатов и методов физики твёрдого тела, математики, дифференциальных уравнений, численных методов, геологии, механики грунтов и др., что способствует достижению поставленной цели. Результаты. Анализ основных методов и способов уплотнения лёссовых просадочных грунтов Северного Кавказа показал, что метод глубинных взрывов является наиболее эффективным, мгновенным и экономически выгодным. Разработана математическая модель уплотнения лёссов глубинными взрывами на основе полуэмпирического уравнения диффузии. Она позволяет рассчитать концентрацию и плотность уплотнённого грунта в результате взрыва за заданное время при заданной температуре. Получены зависимости параметров глубинного взрыва, имеющие большое практическое значение, т. к. позволяют на основе лабораторных испытаний грунтов проектировать камуфлетные взрывы в лёссовых просадочных грунтах.
ИЗМЕНЕНИЕ ЭЛЕМЕНТНОГО СОСТАВА ВЕРХНЕГО ГОРИЗОНТА ПОЧВ ПОД ВОЗДЕЙСТВИЕМ ПРЕДПРИЯТИЙ НЕФТЕГАЗОПЕРЕРАБОТКИ
Authors: Боев Владислав Викторович , Барановская Наталья Владимировна , Боев Виктор Александрович , Джамбаев Мерей Тлеуканович , Шахова Татьяна Сергеевна , Жорняк Лина Владимировна
Published: November 2019

Актуальность исследования обусловлена отсутствием данных по воздействию многих нефтеперерабатывающих заводов и прочих техногенных объектов на прилегающие почвы для конкретных регионов при изученности общей геохимической специфики промышленных отраслей. Цель исследования состоит в выявлении специфики воздействия нефтеперерабатывающих заводов и некоторых прочих техногенных объектов на почвы путем сопоставления элементного состава с данными условно-фоновой территории и кларком. Объекты: почвы Тюменского федерального заказника и территорий расположения Антипинского, Омского, Ачинского, Павлодарского нефтеперерабатывающих заводов, почвы населенных пунктов Томской области и г. Томска, почвы территории, подверженной воздействию бывшего Семипалатинского испытательного полигона. Методы: выбор и подготовка пробных площадок, отбор и подготовка почв, определение элементного состава инструментальным нейтронно-активационным и атомно-абсорбционным методами, интерпретация результатов. Результаты. Исследован элементный состав верхнего горизонта почв восточной части Тюменского федерального заказника, территории расположения Антипинского, Омского, Ачинского, Павлодарского нефтеперерабатывающих заводов, районов расположение предприятий г. Томска, зоны влияния бывшего Семипалатинского испытательного полигона. В результате сравнения с данными для региональных почв выявлена геохимическая специфика каждого техногенного района. Отражено отличие в элементном составе почв, прилегающих к разным нефтеперерабатывающим заводам, что определяется как техническими параметрами самих предприятий, так и экологическими условиями территорий их расположения. Путем сопоставления с кларком и условно-фоновой территорией установлен уровень загрязнения и спектр химических элементов, характеризующихся повышенным содержанием, для названных территорий. На примере Антипинского нефтеперерабатывающего завода изучено пространственное распределение элементов по удаленности и сторонам света от техногенного объекта.
A Comparative Study: Blended Cements Containing Analcime and Clinoptilolite
Authors: Akgün Yasemin
Published: November 2019

The demand to blended cements is increasingly
widespread due to clean environment and energy saving. In the future, this
demand will not be met by currently additives on the market. For this reason,
alternative additives will always be needed. The aim of this study is to
provide a contribution for additive variety used in sustainable blended cement
productions. Therefore, in this study, natural zeolite type clinoptilolite (C)
which is widely used in blended cement production and different natural zeolite
type analcime (A) are compared. These zeolites are natural pozzolans with
volcanic tuff origin. Firstly, pozzolanic activities, mineralogical structures,
physical and chemical properties of zeolites were determined. And then, the
heat of hydration, setting times and compressive strength tests were performed
on samples prepared by using Portland cement (PC) and natural zeolite blended
cements. The replacement ratios of zeolites used in test samples are 0, 10, 30
and 50% of Portland cement weight. According to results of pozzolanic activity
tests, the compressive strengths of lime-zeolite (pozzolan) mixtures were
determined as 6.30MPa for analcime and 9.02MPa for clinoptilolite.  The heats of hydration of blended cements containing
analcime were lower than that of blended cements containing clinoptiloite and
that of Portland cement at all replacement ratios. The setting times of blended
cements containing analcime were determined as shorter than that of blended
cements containing clinoptiloite and that of Portland cement. The compressive
strengths of mortars containing analcime blended cements were slightly higher
than that of mortars containing clinoptilolite blended cements. The optimum
strength values of mortars containing both zeolite blended cements were obtained
at 10% replacement ratio. According to the results obtained from the study,
analcime exhibits similar properties to clinoptilolite. In some cases, due to
these similar properties, analcime may be an alternative additive to
clinoptilolite which is widely used in blended cement industry.
“Hacer mayorazgo infalible de consejeros y ministros graves”. El Señorío de Vizcaya y el proyec­to de fundar un colegio en Salamanca (1638-c. 1660) = ‘To Establish a Mayorazgo of Councillors and Relevant Ministers‘. The Lordship of Biscay and the Project to Found a College in Salamanca (1638-c. 1660)
Authors: Merino Malillos Imanol
Published: November 2019

Resumen: En un contexto de predo­minio de la cultura letrada y del derecho, la presencia de magistrados naturales u ori­ginarios de un territorio en los principales órganos jurisdiccionales de la Corona podía propiciar ventajas a las entidades territoria­les de los lugares de los que aquellos proce­dían, así como a sus paisanos. Para facilitar su promoción, hacia 1638 uno de los letra­dos vizcaínos propuso a las instituciones provinciales fundar un colegio en Salamanca con la ayuda de los hijos de Vizcaya emigra­dos. Una proposición que atrajo la atención de las instituciones vizcaínas.Palabras clave: Historia de las uni­versidades, derecho, letrados, Señorío de Vizcaya, Universidad de Salamanca.Abstract: In a time when law and jus­tice were at the foundation of the society, as it was in the 16th and 17th centuries, the pre­sence of magistrates coming from a land in the main courts of the Spanish Monarchy could provide a wide range of advantages to the ins­titutions of those territories and the people from them. To make easier for their ‘sons’ to achieved those posts, in 1638 a lawyer from Biscay made a proposition to the institutions of Biscay. He set out the foundation of a Colle­ge in Salamanca, that should be set up with the money of the people from Biscay stablished abroad. A proposition that drawn the atten­tion of the institutions of Biscay.Keywords: History of universities, Law, lawyers, Lordship of Biscay, universities, University of Salamanca.
EFEKTIVITAS PROGRAM KLASTER BANK INDONESIA PROVINSI KALIMANTAN TIMUR TERHADAP PRODUKTIVITAS UMKM DI KOTA SAMARINDA
Authors: Hairullah H , Tikawati T , Iswadi Muhammad
Published: November 2019

Usaha Mikro, Kecil dan Menengah (UMKM) mempunyai peran penting dan strategis dalam pembangunan ekonomi nasional, pertumbuhan ekonomi dan penyerapan tenaga kerja. Disamping itu, dalam struktur perekonomian, umumnya UMKM merupakan lapisan pelaku usaha yang paling besar, yang sering juga disebut dengan pelaku ekonomi rakyat. Namun, yang jadi persoalan adalah kenyataan bahwa UMKM tidak dibekali dengan kemampuan sumber daya manusia yang kurang berkualitas, keterbatasan akses pada sumber daya produktif, kurangnya informasi yang akurat dan terkini, finansial dan keterbatasan teknologi. Tujuan dari penelitian ini adalah untuk mengetahui bagaimana strategi dan efektivitas program klaster Bank Indonesia Provinsi Kalimantan Timur dalam mendukung produktivitas UMKM di Desa Lubuk Sawah, Mugirejo Samarinda. Sedangkan metode yang dipakai dalam penelitian adalah penelitian kualitatif. Untuk mendapatkan berbagai data-data dalam penelitian ini, penulis menggunakan metode wawancara, observasi dan dokumentasi. Teknik analisis data menggunakan teknik analisis yang bersumber dari Miles dan Huberman. Terdiri atas pengumpulan data, reduksi data, model data, serta penarikan kesimpulan atau verifikasi. Pemeriksaan keabsahan data menggunakan teknik triangulasi sumber dat metode.. Hasil yang dapat diperoleh dari penelitian ini adalah bahwa strategi yang digunakan dalam pemberian program Klaster Bank Indonesia Provinsi Kalimantan Timur terhadap produktivitas UMKM di Desa Lubuk Sawah, Mugirejo, Samarinda dengan jenis klaster sapi yaitu memberikan pelatihan tentang peternakan, kelembagaan, pemasaran serta pemberian teknologi, kandang sapi terintegras, ruang pertemuan yang digunakan oleh Kelompok Ternak Sapi Damarwulan. Sehingga Program Klaster Bank Indonesia Provinsi Kalimantan Timur untuk Kelompok Sapi Damarwulan sudah berjalan dan terlaksana dengan efektif, ini diperlihatkan dari ilmu atau keterampilan (skill) mengenai peternakan sapi  dan kelembagaan yang bertambah karena adanya pelatihan dari Bank Indonesia Provinsi Kalimantan Timur, serta jumlah produk sapi yang dijual meningkat, omset penjualan yang juga meningkat karena adanya pelatihan atau pemasaran yang telah diberikan, serta pemerintah pun sangatlah senang karena program 2 juta ekor sapi berjalan dengan lancar dibuktikan dengan makin tahun sapi makin bertambah serta pendapatan dan penjualan makin meningkat setiap tahun.
Kata Kunci : Efektivitas, Klaster, Bank Indonesia, UMKM