bluuebunny/crossref_metadata_2025 huggingface dataset:
Myricitrin Ameliorates Hyperglycemia, Glucose Intolerance, Hepatic Steatosis, and Inflammation in High-Fat Diet/Streptozotocin-Induced Diabetic Mice
Authors: Kim Do Yeon , Kim Sang Ryong , Jung Un Ju
Published: March 2020

To test the hypothesis that myricitrin (MYR) improves type 2 diabetes, we examined the effect of MYR on hyperglycemia, glucose intolerance, hepatic steatosis, and inflammation in high-fat diet (HFD) and streptozotocin (STZ)-induced type 2 diabetic mice. Male C57BL/6J mice were randomly divided into three groups: non-diabetic, diabetic control, and MYR (0.005%, w/w)-supplemented diabetic groups. Diabetes was induced by HFD and STZ, and MYR was administered orally for 5 weeks. Myricitrin exerted no significant effects on food intake, body weight, fat weight, or plasma lipids levels. However, MYR significantly decreased fasting blood glucose levels, improved glucose intolerance, and increased pancreatic β-cell mass compared to the diabetic control group. Myricitrin administration also markedly increased glucokinase mRNA expression and activity as well as lowered glucose-6-phosphatase and phosphoenolpyruvate carboxykinase mRNA expression and activity in the liver. In addition, liver weight, hepatic triglyceride content, and lipid droplet accumulation were markedly decreased following MYR administration. These changes were seemingly attributable to the suppression of the hepatic lipogenic enzymes—fatty acid synthase and phosphatidate phosphohydrolase. Myricitrin also significantly lowered plasma MCP-1 and TNF-α levels and the mRNA expression of hepatic pro-inflammatory genes. These results suggest that MYR has anti-diabetic potential.
Crosstalk Among Circadian Rhythm, Obesity and Allergy
Authors: Orihara Kanami , Haraguchi Atsushi , Shibata Shigenobu
Published: March 2020

The circadian clock system works not only as a cellular time-keeper but also as a coordinator for almost all physiological functions essential to maintaining human health. Therefore, disruptions or malfunctions of this system can cause many diseases and pre-symptomatic conditions. Indeed, previous studies have indicated that disrupted clock gene expression rhythm is closely related to obesity, and that allergic diseases can be regulated by controlling peripheral clocks in organs and tissues. Moreover, recent studies have found that obesity can lead to immune disorders. Accordingly, in this review, we assess the connection between obesity and allergy from the point of view of the circadian clock system anew and summarize the relationships among the circadian clock system, obesity, and allergy.
At-Hook Motif Nuclear Localised Protein 18 as a Novel Modulator of Root System Architecture
Authors: Širl Marek , Šnajdrová Tereza , Gutiérrez-Alanís Dolores , Dubrovsky Joseph G. , Vielle-Calzada Jean Phillipe , Kulich Ivan , Soukup Aleš
Published: March 2020

The At-Hook Motif Nuclear Localized Protein (AHL) gene family encodes embryophyte-specific nuclear proteins with DNA binding activity. They modulate gene expression and affect various developmental processes in plants. We identify AHL18 (At3G60870) as a developmental modulator of root system architecture and growth. AHL18 is involved in regulation of the length of the proliferation domain and number of dividing cells in the root apical meristem and thereby, cell production. Both primary root growth and lateral root development respond according to AHL18 transcription level. The ahl18 knock-out plants show reduced root systems due to a shorter primary root and a lower number of lateral roots. This change results from a higher number of arrested and non-developing lateral root primordia (LRP) rather than from a decreased LRP initiation. The over-expression of AHL18 results in a more extensive root system, longer primary roots, and increased density of lateral root initiation events. AHL18 is thus involved in the formation of lateral roots at both LRP initiation and their later development. We conclude that AHL18 participates in modulation of root system architecture through regulation of root apical meristem activity, lateral root initiation and emergence; these correspond well with expression pattern of AHL18.
Effects of Osiris9a on Silk Properties in Bombyx mori Determined by Transgenic Overexpression
Authors: Cheng Tingcai , Zhang Xia , Peng Zhangchuan , Fan Yinfeng , Zhang Lin , Liu Chun
Published: March 2020

Osiris is an insect-specific gene family with multiple biological roles in development, phenotypic polymorphism, and protection. In the silkworm, we have previously identified twenty-five Osiris genes with high evolutionary conservation and remarkable synteny among several insects. Bombxy mori Osiris9a (BmOsi9a) is expressed only in the silk gland, particularly in the middle silk gland (MSG). However, the biological function of BmOsi9a is still unknown. In this study, we overexpressed BmOsi9a in the silk gland by germline transgene expression. BmOsi9a was overexpressed not only in the MSG but also in the posterior silk gland (PSG). Interestingly, BmOsi9a could be secreted into the lumen in the MSG but not in the PSG. In the silk fiber, overexpressed BmOsi9a interacted with Sericin1 in the MSG, as confirmed by a co-immunoprecipitation assay. The overexpression of BmOsi9a altered the secondary structure and crystallinity of the silk fiber, thereby changing the mechanical properties. These results provide insight into the mechanisms underlying silk proteins secretion and silk fiber formation.
Leucine-Rich Repeat Kinase 2 Controls Inflammatory Cytokines Production through NF-κB Phosphorylation and Antigen Presentation in Bone Marrow-Derived Dendritic Cells
Authors: Kubo Makoto , Nagashima Ryuichi , Kurihara Mitsue , Kawakami Fumitaka , Maekawa Tatsunori , Eshima Koji , Ohta Etsuro , Kato Hirotomo , Obata Fumiya
Published: March 2020

Leucine-rich repeat kinase 2 (LRRK2) is the causal molecule of familial Parkinson’s disease. Although the characteristics of LRRK2 have gradually been revealed, its true physiological functions remain unknown. LRRK2 is highly expressed in immune cells such as B2 cells and macrophages, suggesting that it plays important roles in the immune system. In the present study, we investigate the roles of LRRK2 in the immune functions of dendritic cells (DCs). Bone marrow-derived DCs from both C57BL/6 wild-type (WT) and LRRK2 knockout (KO) mice were induced by culture with granulocyte/macrophage-colony stimulating factor (GM/CSF) in vitro. We observed the differentiation of DCs, the phosphorylation of the transcriptional factors NF-κB, Erk1/2, and p-38 after lipopolysaccharide (LPS) stimulation and antigen-presenting ability by flow cytometry. We also analyzed the production of inflammatory cytokines by ELISA. During the observation period, there was no difference in DC differentiation between WT and LRRK2-KO mice. After LPS stimulation, phosphorylation of NF-κB was significantly increased in DCs from the KO mice. Large amounts of inflammatory cytokines were produced by DCs from KO mice after both stimulation with LPS and infection with Leishmania. CD4+ T-cells isolated from antigen-immunized mice proliferated to a significantly greater degree upon coculture with antigen-stimulated DCs from KO mice than upon coculture with DCs from WT mice. These results suggest that LRRK2 may play important roles in signal transduction and antigen presentation by DCs.
Biopolymer-Based Microcarriers for Three-Dimensional Cell Culture and Engineered Tissue Formation
Authors: Huang Lixia , Abdalla Ahmed M.E. , Xiao Lin , Yang Guang
Published: March 2020

The concept of three-dimensional (3D) cell culture has been proposed to maintain cellular morphology and function as in vivo. Among different approaches for 3D cell culture, microcarrier technology provides a promising tool for cell adhesion, proliferation, and cellular interactions in 3D space mimicking the in vivo microenvironment. In particular, microcarriers based on biopolymers have been widely investigated because of their superior biocompatibility and biodegradability. Moreover, through bottom-up assembly, microcarriers have opened a bright door for fabricating engineered tissues, which is one of the cutting-edge topics in tissue engineering and regeneration medicine. This review takes an in-depth look into the recent advancements of microcarriers based on biopolymers—especially polysaccharides such as chitosan, chitin, cellulose, hyaluronic acid, alginate, and laminarin—for 3D cell culture and the fabrication of engineered tissues based on them. The current limitations and potential strategies were also discussed to shed some light on future directions.
Improvement of Alcohol-Poisoning Symptoms in Mice by the Oral Administration of Live Lactobacillus plantarum SN13T Cells
Authors: Noda Masafumi , Maruyama Masafumi , Danshiitsoodol Narandalai , Higashikawa Fumiko , Sugiyama Masanori
Published: March 2020

A clinical study carried out previously by our group has demonstrated that yogurt manufactured with a plant-derived lactic acid bacterium, Lactobacillus plantarum SN13T, significantly reduces the γ-glutamyl transpeptidase (γ-GTP) level as a liver-function parameter. In the present study, we show that with the oral administration of live SN13T cells, alcohol-poisoning symptoms in mice are improved, and the condition does not become fatal. However, prior to the simultaneous administration with ethanol, when the cells were heat-killed or sonicated, the improvement was not observed, and almost all of the mice died. In addition, the dysbiosis of the intestinal microbiota observed in the mice administered with ethanol was restored by simultaneous administration with live SN13T cells. Furthermore, by analyzing the metabolites detected in contents from the mouse cecum, it was demonstrated that the increase in nonvolatile putrefactive amines observed in the ethanol-administration group was reduced by simultaneous administration with live SN13T cells. Judging from these results, the lactic acid bacterial cells capable of reaching the living bowels prevent ethanol-induced poisoning and restore the intestinal microbiota.
Near-Field Simulations of Film Cooling with a Modified DES Model
Authors: Yu Feiyan , Yavuzkurt Savas
Published: March 2020

Modeling the heat transfer characteristics of highly turbulent flow in gas turbine film cooling is important for providing better insights and engineering solutions to the film cooling problem. This study proposes a modified detached eddy simulation (DES) model for better film cooling simulations. First, spatially varying anisotropic eddy viscosity is found from the results of the large eddy simulation (LES) of film cooling. Then the correlation for eddy viscosity anisotropy ratio has been established based on the LES results and is proposed as the modification approach for the DES model. The modified DES model has been tested for the near-field film cooling simulations under different blowing ratios. Detailed comparisons of the centerline and 2D film cooling effectiveness indicate that the modified DES model enhances the spanwise spreading of the temperature field. The DES model leads to deviations of 62.4%, 39.8%, and 33.5% from the experimental centerline effectiveness under blowing ratios of 0.5, 1.0, and 1.5, respectively, while the modified DES reduces the deviations to 51.5%, 26.7%, and 28.9%. The modified DES model provides a promising approach for film cooling numerical simulations. It embraces the advantage of LES in resolving detailed vortical structure dynamics with a moderate computational cost. It also significantly improves the original DES model on the spanwise counter rotating vortex pair (CRVP) spreading, mixing, and effectiveness prediction.
Self-Assessment of Driving Style and the Willingness to Share Personal Information
Authors: Pugnetti Carlo , Elmer Sandra
Published: March 2020

The availability of better behavioral information about their customer portfolios holds the promise for different and more accurate pricing models for insurers. Changes in pricing, however, are always fraught with danger for insurers, as they enter long-term commitments with incomplete historical information. On the other hand, sharing personal information is still viewed with skepticism by consumers. Which type of personal information are consumers willing to share with insurers, and for what purpose? How would they like to be rewarded for this openness? For insurers, how will the transition shift their risk portfolios? This paper addresses these questions for auto insurance, particularly how the self-assessment of one’s driving style impacts this dynamic. In a survey of approximately 900 Swiss residents, we found that offering a compensation, especially premium discounts, but also services, significantly improves willingness to share information. Higher trust in insurance increases sharing. Women and younger people are more willing to share information. On the other hand, customers are less willing to disclose, to insurers, information not traditionally associated with insurance. The self-assessment of driving style also plays a significant role. More risk-averse driving styles are correlated with higher sharing. Conversely, riskier driving styles are correlated with lower sharing. This result is significant for insurers, as new data-driven pricing and services models should tend to attract less risky customer portfolios.
Uniaxial Compressive Behavior of Concrete Columns Confined with Superelastic Shape Memory Alloy Wires
Authors: Hong Chenkai , Qian Hui , Song Gangbing
Published: March 2020

Superelastic shape memory alloy (SMA) exhibits the ability to undergo large deformations before reverting back to its undeformed shape following the removal of the load. This unique property underlies its great potential in the seismic design and retrofitting of structure members. In this paper, superelastic SMA wires were utilized to confine concrete cylinders to enhance their axial compressive behavior. The axial carrying and deformation capacities of SMA-confined concrete cylinders are assessed by uniaxial compression testing on a total of eight SMA-confined concrete columns and one unconfined column. The influence of the amount of SMA and the prestrain level of SMA wires, as well as the reinforcing mode, on the axial carrying and deformation capacity of confined concrete columns were considered. The analysis focuses on the axial carrying capacity and deformation performance of concrete columns reinforced with superelastic SMA under different loading conditions. Based on the experimental data and analysis results, it is found that superelastic SMA wires can increase the axial loading capacity and enhance deformation performance of concrete columns. Under the same loading condition, the ultimate bearing capacity of SMA-confined concrete columns increases as the increasing of the amount of SMA wire. The results of this study verify the effectiveness of superelastic SMA in enhancing the loading capacity and deformation behavior of concrete cylinders.
Effect of Oxide Particles on Microstructure and Mechanical Properties of the 45 Carbon Structural Steel
Authors: Chen Bin , Feng Jianhang , Zhai Yongzhen , Sun Zhonghua , Liu Hongbo , Jiang Yanfei , He Jun , Peng Huifen
Published: March 2020

Striking difference in density between the oxide and the steel results in difficulty in preparing oxide dispersion strengthened steel with large size parts or materials. In this research, Al2O3 and TiO2 particles were initially milled with the 20 steel, and then the mixture was heated to a molten state to form a master alloy, which was used as a raw material for further preparation of the object steel. It was found that homogeneous distribution of the oxide particles was obtained in the mass production of the steel. Moreover, the obtained 45 carbon structural steel presents fine microstructures, together with improved mechanical properties, especially the impact ductility. This should be attributable to the transformation from the introduced micro-size oxide particles to the nano ones, which act as heterogeneous nucleants that play an important role in grain refinement and dispersion strengthening for the steel, during the remelting of the master alloy.
Experimental Insights into Concrete Flow-Regimes Subject to Shear-Induced Particle Migration (SIPM) during Pumping
Authors: Fataei Shirin , Secrieru Egor , Mechtcherine Viktor
Published: March 2020

In this paper, the authors have focused on shear-induced particle migration (SIPM), its effect on concrete flow patterns, and lubricating layer formation during pumping. For this purpose, various volume-fractions ϕ of aggregates were selected. The particle migration was analyzed by applying two methods: sampling hardened concrete exposed to pumping and performing X-ray microcomputed tomography (μCT) and image analysis to determine the thickness of the lubricating layer due to SIPM. The results indicate that the first approach is unsuitable due to the nearly equal molecular density of particles and matrix. The second approach indicated that the actual thickness of the lubricating layer depends on the discharge rate as well as on ϕ and viscosity of concrete bulk; hence, it cannot be defined as a constant parameter for all concrete mixtures. Additionally, the concrete pipe-flow pattern, i.e., plug versus shear flow, was captured and studied while considering pumping pressure and discharge rate. It was concluded that particle migration is essential in the cases of both flowable and very flowable concretes with a high volume-fraction of solids. The changes in rheological properties caused by SIPM are severe enough to influence the definition of the flow pattern as plug or shear and the discharge rate of pumped concrete as well.
Investigation on an Absorbing Layer Suitable for a Noise-Reducing Two-Layer Pavement
Authors: Faßbender Sabine , Oeser Markus
Published: March 2020

A polyurethane-based rubber-modified layer within a road superstructure leads to absorption of traffic emissions. Noise emissions have quite a negative effect on society, as they lead to high stress levels and health risks for people. Therefore, constructional methods of noise-reducing road layers have been developed before. This research paper focuses on the questions whether the existing noise-reducing road constructions, which have a low durability, can be optimized in terms of a longer duration while simultaneously maintaining the noise-reducing effects. Within this research, a large parametric study contributed to an optimal solution of a noise-reducing and durable layer. We found that noise absorption is mainly dependent of the void content of the pavement and its flexibility. Also, a result is that the durability of a road layer is based on the properties of the binder as well as the composition of the mixture, i.e., the grading curve. As we used polyurethane binders within our mixtures, which have a low dependency on regular environmental temperatures after their complete chemical reaction, we can imply a low temperature dependence of the entire polyurethane asphalt mixture. Based on these results, the construction of a noise-reducing and durable road layer is a great solution. The application of such road layers leads to lower traffic emissions at major hotspots. These might be urban highways, where the infrastructure is too tight to build noise barriers, enclosures or tunnels.
Effect of CeO2 Nanoparticles on Interface of Cu/Al2O3 Ceramic Clad Composites
Authors: Fu YaBo , Chen HaoNan , Cao ZhiQiang , Huo YanQiu
Published: March 2020

Cu/Al2O3 ceramic clad composites are widely used in electronic packaging and electrical contacts. However, the conductivity and strength of the interfacial layer are not fit for the demands. So CeO2 nanoparticles 24.3 nm in size, coated on Al2O3 ceramic, promote a novel CeO2–Cu2O–Cu system to improve the interfacial bonded strength. Results show that the atom content of O is increased to approximately 30% with the addition of CeO2 nanoparticles compared with the atom content without CeO2 in the interfacial layer of Cu/Al2O3 ceramic clad composites. CeO2 nanoparticles coated on the surface of Al2O3 ceramics can easily diffuse into the metallic Cu layer. CeO2 nanoparticles can accelerate to form the eutectic liquid of Cu2O–Cu as they have strong functions of storing and releasing O at an Ar pressure of 0.12 MPa. The addition of CeO2 nanoparticles is beneficial for promoting the bonded strength of the Cu/Al2O3 ceramic clad composites. The bonded strength of the interface coated with nanoparticles of CeO2 is increased to 20.8% compared with that without CeO2; moreover, the electric conductivity on the side of metallic Cu is 95% IACS. The study is of great significance for improving properties of Cu/Al2O3 ceramic clad composites.
Post-Fire Characteristics of Concrete Beams Reinforced with Hybrid FRP Bars
Authors: Protchenko Kostiantyn , Szmigiera Elżbieta
Published: March 2020

One of the main concerns of experimental and numerical investigations regarding the behavior of fiber-reinforced polymer reinforced concrete (FRP-RC) members is their fire resistance to elevated temperatures and structural performance at and after fire exposure. However, the data currently available on the behavior of fiber-reinforced polymer (FRP) reinforced members related to elevated temperatures are scarce, specifically relating to the strength capacity of beams after being subjected to elevated temperatures. This paper investigates the residual strength capacity of beams strengthened internally with various (FRP) reinforcement types after being subjected to high temperatures, reflecting the conditions of a fire. The testing was made for concrete beams reinforced with three different types of FRP bars: (i) basalt-FRP (BFRP), (ii) hybrid FRP with carbon and basalt fibers (HFRP) and (iii) nano-hybrid FRP (nHFRP), with modification of the epoxy matrix of the rebar. Tested beams were first loaded at 50% of their ultimate strength capacity, then unloaded before being heated in a furnace and allowed to cool, and finally reloaded flexurally until failure. The results show an atypical behavior observed for HFRP bars and nHFRP bars reinforced beams, where after a certain temperature threshold the deflection began to decrease. The authors suggest that this phenomenon is connected with the thermal expansion coefficient of the carbon fibers present in HFRP and nHFRP bars and therefore creep can appear in those fibers, which causes an effect of “prestressing” of the beams.
Hardness and Wear Resistance of Dental Biomedical Nanomaterials in a Humid Environment with Non-Stationary Temperatures
Authors: Pieniak Daniel , Walczak Agata , Walczak Mariusz , Przystupa Krzysztof , Niewczas Agata M.
Published: March 2020

This study discusses a quantitative fatigue evaluation of polymer–ceramic composites for dental restorations, i.e., commercial material (Filtek Z550) and experimental materials Ex-nano (G), Ex-flow (G). Their evaluation is based on the following descriptors: microhardness, scratch resistance, and sliding wear. In order to reflect factors of environmental degradation conditions, thermal fatigue was simulated with a special computer-controlled device performing algorithms of thermocycling. Specimens intended for the surface strength and wear tests underwent 104 hydrothermal fatigue cycles. Thermocycling was preceded by aging, which meant immersing the specimens in artificial saliva at 37 °C for 30 days. Microhardness tests were performed with the Vickers hardness test method. The scratch test was done with a Rockwell diamond cone indenter. Sliding ball-on-disc friction tests were performed against an alumina ball in the presence of artificial saliva. A direct positive correlation was found between thermocycling fatigue and microhardness. The dominant mechanism of the wear of the experimental composites after thermocycling is the removal of fragments of the materials in the form of flakes from the friction surface (spalling). Hydrothermal fatigue is synergistic with mechanical fatigue.
Experimental Examination of Electrical Characteristics for Portland Cement Mortar Frost Damage Evaluation
Authors: Wang Yi , Ueda Tamon , Gong Fuyuan , Zhang Dawei , Wang Zhao
Published: March 2020

Electrical measurements are promising for evaluation of frost damage of concrete, but the index is still controversial. In this paper, to propose an efficient index, various electrical characteristics were examined to correlate them with the mechanical property degradation of meso-scale mortar samples due to combined effects of sodium chloride and freeze–thaw cycles (FTCs). While the electrical responses of specimens were measured during FTCs, the mechanical properties were obtained from three-point bending tests after FTCs. Typical microstructural change after the damage was also analyzed by using a water absorption test. The results showed that no clear degradation tendency was observed for electrical resistivity at the lowest temperature, the activation energy or the freezing/thawing point change with the FTCs. The reduction in electrical resistivity at reference temperature has a consistent tendency with that of elastic modulus and flexural strength, thus can be an efficient index for quantitative frost damage evaluation. The change due to salt-frost damage is mainly due to the increase of connectivity rather than porosity.
Antioxidant Peptides from the Protein Hydrolysate of Monkfish (Lophius litulon) Muscle: Purification, Identification, and Cytoprotective Function on HepG2 Cells Damage by H2O2
Authors: Hu Xiao-Meng , Wang Yu-Mei , Zhao Yu-Qin , Chi Chang-Feng , Wang Bin
Published: March 2020

In the work, defatted muscle proteins of monkfish (Lophius litulon) were separately hydrolyzed by pepsin, trypsin, and in vitro gastrointestinal (GI) digestion methods, and antioxidant peptides were isolated from proteins hydrolysate of monkfish muscle using ultrafiltration and chromatography processes. The antioxidant activities of isolated peptides were evaluated using radical scavenging and lipid peroxidation assays and H2O2-induced model of HepG2 cells. In which, the cell viability, reactive oxygen species (ROS) content, and antioxidant enzymes and malondialdehyde (MDA) levels were measured for evaluating the protective extent on HepG2 cells damaged by H2O2. The results indicated that the hydrolysate (MPTH) prepared using in vitro GI digestion method showed the highest degree of hydrolysis (27.24 ± 1.57%) and scavenging activity on a 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical (44.54 ± 3.12%) and hydroxyl radical (41.32 ± 2.73%) at the concentration of 5 mg protein/mL among the three hydrolysates. Subsequently, thirteen antioxidant peptides (MMP-1 to MMP-13) were isolated from MPTH. According to their DPPH radical and hydroxyl radical scavenging activity, three peptides with the highest antioxidant activity were selected and identified as EDIVCW (MMP-4), MEPVW (MMP-7), and YWDAW (MMP-12) with molecular weights of 763.82, 660.75, and 739.75 Da, respectively. EDIVCW, MEPVW, and YWDAW showed high scavenging activities on DPPH radical (EC50 0.39, 0.62, and 0.51 mg/mL, respectively), hydroxyl radical (EC50 0.61, 0.38, and 0.32 mg/mL, respectively), and superoxide anion radical (EC50 0.76, 0.94, 0.48 mg/mL, respectively). EDIVCW and YWDAW showed equivalent inhibiting ability on lipid peroxidation with glutathione in the linoleic acid model system. Moreover, EDIVCW, MEPVW, and YWDAW had no cytotoxicity to HepG2 cells at the concentration of 100.0 µM and could concentration-dependently protect HepG2 cells from H2O2-induced oxidative damage through decreasing the levels of reactive oxygen species (ROS) and MDA and activating intracellular antioxidant enzymes of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px). These present results indicated that the protein hydrolysate and isolated antioxidant peptides from monkfish muscle, especially YWDAW could serve as powerful antioxidants applied in the treatment of some liver diseases and healthcare products associated with oxidative stress.
Biology and Pathogenesis of Staphylococcus Infection
Authors: Ebani Valentina Virginia
Published: March 2020

Members of the genus Staphylococcus still represent a topic of great relevance due to the numerous types of infections they cause in humans and animals [...]
Fe-Ti Oxide Assemblages from the Contact-Metamorphosed Mafic-Ultramafic Rocks of the Sedova Zaimka Intrusion (Western Siberia, Russia): The Tracking of Metamorphic Transformations
Authors: Svetlitskaya Tatyana V. , Nevolko Peter A. , Fominykh Pavel A.
Published: March 2020

Studies that track and compiled transformations of ilmenite and magnetite under conditions of different metamorphic grades are still very limited. The Sedova Zaimka intrusion in Western Siberia (Russia) is a good example to examine the effect of contact metamorphism from greenschist to low-amphibolite facies on magmatic Fe-Ti oxide minerals, as this small mafic-ultramafic body is located within contact metamorphic aureole of a large granite pluton. In the Sedova Zaimka, ilmenite with little to no magnetite is present as an accessory dissemination throughout metamorphosed rocks. Ilmenite is variably enriched in MnO (1 to 13.3 wt%) and depleted in MgO (less than 0.3 wt%), suggesting that its primary magmatic composition has been unevenly modified by diffusion re-equilibrium with coexisting metamorphic silicates. The elevated content of ZnO (up to 0.5 wt%) and NiO (up to 0.4 wt%) in ilmenite suggests that both ZnO and NiO, like MnO, must be strongly partitioned into ilmenite relatively to silicate minerals under the reducing contact metamorphic conditions, if chromite is absent. The textural observations of ilmenite–sulfide and ilmenite–titanite–sulfide relationships indicate that Ti-magnetite, in contrast to ilmenite, is an unstable phase in the presence of sulfur-rich reduced metamorphic fluids and is completely replaced by sulfides, with the development of specific “octahedral meshes” of ilmenite in sulfides.
Prunellae Spica Extract Suppresses Teratoma Formation of Pluripotent Stem Cells through p53-Mediated Apoptosis
Authors: Kim Aeyung , Lee Seo-Young , Seo Chang-Seob , Chung Sun-Ku
Published: March 2020

Induced pluripotent stem cells (iPSCs) have similar properties to embryonic stem cells in terms of indefinite self-renewal and differentiation capacity. After in vitro differentiation of iPSCs, undifferentiated iPSCs (USCs) may exist in cell therapy material and can form teratomas after in vivo transplantation. Selective elimination of residual USCs is, therefore, very important. Prunellae Spica (PS) is a traditional medicinal plant that has been shown to exert anti-cancer, antioxidant, and anti-inflammatory activities; however, its effects on iPSCs have not been previously characterized. In this study, we find that ethanol extract of PS (EPS) effectively induces apoptotic cell death of USCs through G2/M cell cycle arrest, generation of intracellular reactive oxygen species, alteration of mitochondrial membrane potentials, and caspase activation of USCs. In addition, EPS increases p53 accumulation and expression of its downstream targets. In p53 knockout (KO) iPSCs, the EPS did not induce apoptosis, indicating that EPS-mediated apoptosis of USCs was p53-dependent. In addition, EPS was not genotoxic towards iPSCs-derived differentiated cells. EPS treatment before injection efficiently prevented in ovo teratoma formation of p53 wild-type (WT) iPSCs but not p53KO iPSCs. Collectively, these results indicate that EPS has potent anti-teratoma activity and no genotoxicity to differentiated cells. It can, therefore, be used in the development of safe and efficient iPSC-based cell therapies.
Implementation of Dietary Reference Intake Standards in Prison Menus in Poland
Authors: Stanikowski Piotr , Michalak-Majewska Monika , Domagała Dorota , Jabłońska-Ryś Ewa , Sławińska Aneta
Published: March 2020

Adequate nutrition in prisons should constantly be monitored due to the limited possibilities of external control as well as the low catering budget for prison meals and poorly defined requirements in this regard. The aim of the study was to assess the nutritional value of meals served in Polish prisons. Using a computer program, 14-day regular and bland diets from 30 prisons were analyzed. The energy value of the meals and the percentage of energy provided by protein, fat, and carbohydrate contained therein were found to meet the recommendations of the Polish National Food and Nutrition Institute. The amount of minerals supplied with the diet did not cover the recommended dietary allowance (RDA) in the case of calcium and magnesium. Particularly disturbing was the excessive supply of sodium in the regular and bland diets, which covered 537% and 311% of the dietary reference intake (DRI), respectively, as well as phosphorus (194 and 192% of RDA). The largest vitamin deficiencies were recorded for vitamins D and C and folate. An especially excessive supply was observed for vitamins A and B12. The type of diet significantly differentiated the average content of over half of the analyzed components, whereas the season of the year turned out to be statistically insignificant. The results of the present investigations indicate a need for development of more accurate legal provisions to regulate the nutrition in Polish prisons in terms of not only the energy value and macronutrient supply but also the intake of minerals and vitamins.
Transcriptome Analysis of The Inflammatory Responses of Bovine Mammary Epithelial Cells: Exploring Immunomodulatory Target Genes for Bovine Mastitis
Authors: Islam Md. Aminul , Takagi Michihiro , Fukuyama Kohtaro , Komatsu Ryoya , Albarracin Leonardo , Nochi Tomonori , Suda Yoshihito , Ikeda-Ohtsubo Wakako , Rutten Victor , Eden Willem van , Villena Julio , Aso Hisashi , Kitazawa Haruki
Published: March 2020

Bovine mastitis is the inflammatory reaction of the mammary gland and is commonly caused by bacterial infections in high-yielding dairy cows. The detailed investigation of the immunotranscriptomic response of bovine mammary epithelial (BME) cells to pattern recognition receptors (PRRs) activation by microbial-associated molecular patterns (MAMPs) can be of great importance for understanding the innate immune defense mechanisms, and for exploring the immunomodulatory candidate genes. In this work, we investigated the transcriptome modifications of BME cells after the in vitro stimulation with Escherichia coli derived lipopolysaccharide (LPS) and heat-killed Staphylococcus aureus JE2 and S. aureus SA003. In addition, the effect of Pam3CSK4 (a synthetic triacylated lipopeptide that activates Toll-like receptor 2 (TLR2)), and the intracellular chemotactic protein cyclophilin A (CyPA), which is secreted by BME cells during mastitis, in the expression changes of selected cytokines and chemokines were evaluated by qPCR. Microarray analysis identified 447, 465 and 520 differentially expressed genes (DEGs) in the BME cells after LPS, S. aureus JE2 and S. aureus SA003 stimulation, respectively. A major differential response in the inflammatory gene expression was noticed between the stimulation of LPS and S. aureus strains. Unlike the S. aureus strains, LPS stimulation resulted in significant upregulation of CCL2, CXCL2, CXCL3, CXCL8, IL1α and IL1β, which were confirmed by qPCR analysis. Pam3CSK4 was not able to induce significant changes in the expression of cytokines and chemokines in challenged BME cells. The exogenous CyPA administration was able to upregulate CXCL2, CXCL3, CXCL8, IL1α and IL1β expression in BME cells indicating its ability to promote inflammation. The identification of transcriptional markers of mastitis specific for individual inflammatory factors such as LPS, Pam3CSK4 or CyPA, which can be evaluated in vitro in BME cells, may enable the development of novel diagnostics and/or immunomodulatory treatments, providing new tools for the effective management of mastitis in dairy cows. The results of this work are an advance in this regard.
Estimating Ground-Level Particulate Matter in Five Regions of China Using Aerosol Optical Depth
Authors: Zeng Qiaolin , Tao Jinhua , Chen Liangfu , Zhu Hao , Zhu SongYan , Wang Yang
Published: March 2020

Aerosol optical depth (AOD) has been widely used to estimate near-surface particulate matter (PM). In this study, ground-measured data from the Campaign on Atmospheric Aerosol Research network of China (CARE-China) and the Aerosol Robotic Network (AERONET) were used to evaluate the accuracy of Visible Infrared Imaging Radiometer Suite (VIIRS) AOD data for different aerosol types. These four aerosol types were from dust, smoke, urban, and uncertain and a fifth “type” was included for unclassified (i.e., total) aerosols. The correlation for dust aerosol was the worst (R2 = 0.15), whereas the correlations for smoke and urban types were better (R2 values of 0.69 and 0.55, respectively). The mixed-effects model was used to estimate the PM2.5 concentrations in Beijing–Tianjin–Hebei (BTH), Sichuan–Chongqing (SC), the Pearl River Delta (PRD), the Yangtze River Delta (YRD), and the Middle Yangtze River (MYR) using the classified aerosol type and unclassified aerosol type methods. The results suggest that the cross validation (CV) of different aerosol types has higher correlation coefficients than that of the unclassified aerosol type. For example, the R2 values for dust, smoke, urban, uncertain, and unclassified aerosol types BTH were 0.76, 0.85, 0.82, 0.82, and 0.78, respectively. Compared with the daily PM2.5 concentrations, the air quality levels estimated using the classified aerosol type method were consistent with ground-measured PM2.5, and the relative error was low (most RE was within ±20%). The classified aerosol type method improved the accuracy of the PM2.5 estimation compared to the unclassified method, although there was an overestimation or underestimation in some regions. The seasonal distribution of PM2.5 was analyzed and the PM2.5 concentrations were high during winter, low during summer, and moderate during spring and autumn. Spatially, the higher PM2.5 concentrations were predominantly distributed in areas of human activity and industrial areas.
Assessing the Link between Human Modification and Changes in Land Surface Temperature in Hainan, China Using Image Archives from Google Earth Engine
Authors: Chu Lixia , Oloo Francis , Bergstedt Helena , Blaschke Thomas
Published: March 2020

In many areas of the world, population growth and land development have increased demand for land and other natural resources. Coastal areas are particularly susceptible since they are conducive for marine transportation, energy production, aquaculture, marine tourism and other activities. Anthropogenic activities in the coastal areas have triggered unprecedented land use change, depletion of coastal wetlands, loss of biodiversity, and degradation of other vital ecosystem services. The changes can be particularly drastic for small coastal islands with rich biodiversity. In this study, the influence of human modification on land surface temperature (LST) for the coastal island Hainan in Southern China was investigated. We hypothesize that for this island, footprints of human activities are linked to the variation of land surface temperature, which could indicate environmental degradation. To test this hypothesis, we estimated LST changes between 2000 and 2016 and computed the spatio-temporal correlation between LST and human modification. Specifically, we classified temperature data for the four years 2000, 2006, 2012 and 2016 into 5 temperature zones based on their respective mean and standard deviation values. We then assessed the correlation between each temperature zone and a human modification index computed for the year 2016. Apart from this, we estimated mean, maximum and the standard deviation of annual temperature for each pixel in the 17 years to assess the links with human modification. The results showed that: (1) The mean LST temperature in Hainan Island increased with fluctuations from 2000 to 2016. (2) The moderate temperature zones were dominant in the island during the four years included in this study. (3) A strong positive correlation of 0.72 between human modification index and mean and maximum LST temperature indicated a potential link between human modification and mean and maximum LST temperatures over the 17 years of analysis. (4) The mean value of human modification index in the temperature zones in 2016 showed a progressive rise with 0.24 in the low temperature zone, 0.33 in the secondary moderate, 0.45 in the moderate, 0.54 in the secondary high and 0.61 in the high temperature zones. This work highlighted the potential value of using large and multi-temporal earth observation datasets from cloud platforms to assess the influence of human activities in sensitive ecosystems. The results could contribute to the development of sustainable management and coastal ecosystems conservation plans.
X-Net-Based Radar Data Assimilation Study over the Seoul Metropolitan Area
Authors: Lee Ji-Won , Min Ki-Hong , Lee Young-Hee , Lee GyuWon
Published: March 2020

This study investigates the ability of the high-resolution Weather Research and Forecasting (WRF) model to simulate summer precipitation with assimilation of X-band radar network data (X-Net) over the Seoul metropolitan area. Numerical data assimilation (DA) experiments with X-Net (S- and X-band Doppler radar) radial velocity and reflectivity data for three events of convective systems along the Changma front are conducted. In addition to the conventional assimilation of radar data, which focuses on assimilating the radial velocity and reflectivity of precipitation echoes, this study assimilates null-echoes and analyzes the effect of null-echo data assimilation on short-term quantitative precipitation forecasting (QPF). A null-echo is defined as a region with non-precipitation echoes within the radar observation range. The model removes excessive humidity and four types of hydrometeors (wet and dry snow, graupel, and rain) based on the radar reflectivity by using a three-dimensional variational (3D-Var) data assimilation technique within the WRFDA system. Some procedures for preprocessing radar reflectivity data and using null-echoes in this assimilation are discussed. Numerical experiments with conventional radar DA over-predicted the precipitation. However, experiments with additional null-echo information removed excessive water vapor and hydrometeors and suppressed erroneous model precipitation. The results of statistical model verification showed improvements in the analysis and objective forecast scores, reducing the amount of over-predicted precipitation. An analysis of a contoured frequency by altitude diagram (CFAD) and time–height cross-sections showed that increased hydrometeors throughout the data assimilation period enhanced precipitation formation, and reflectivity under the melting layer was simulated similarly to the observations during the peak precipitation times. In addition, overestimated hydrometeors were reduced through null-echo data assimilation.
Remote Sensing Derived Indices for Tracking Urban Land Surface Change in Case of Earthquake Recovery
Authors: Derakhshan Sahar , Cutter Susan L. , Wang Cuizhen
Published: March 2020

The study of post-disaster recovery requires an understanding of the reconstruction process and growth trend of the impacted regions. In case of earthquakes, while remote sensing has been applied for response and damage assessment, its application has not been investigated thoroughly for monitoring the recovery dynamics in spatially and temporally explicit dimensions. The need and necessity for tracking the change in the built-environment through time is essential for post-disaster recovery modeling, and remote sensing is particularly useful for obtaining this information when other sources of data are scarce or unavailable. Additionally, the longitudinal study of repeated observations over time in the built-up areas has its own complexities and limitations. Hence, a model is needed to overcome these barriers to extract the temporal variations from before to after the disaster event. In this study, a method is introduced by using three spectral indices of UI (urban index), NDVI (normalized difference vegetation index) and MNDWI (modified normalized difference water index) in a conditional algebra, to build a knowledge-based classifier for extracting the urban/built-up features. This method enables more precise distinction of features based on environmental and socioeconomic variability, by providing flexibility in defining the indices’ thresholds with the conditional algebra statements according to local characteristics. The proposed method is applied and implemented in three earthquake cases: New Zealand in 2010, Italy in 2009, and Iran in 2003. The overall accuracies of all built-up/non-urban classifications range between 92% to 96.29%; and the Kappa values vary from 0.79 to 0.91. The annual analysis of each case, spanning from 10 years pre-event, immediate post-event, and until present time (2019), demonstrates the inter-annual change in urban/built-up land surface of the three cases. Results in this study allow a deeper understanding of how the earthquake has impacted the region and how the urban growth is altered after the disaster.
A Systematic Review on the Use of Wearable Body Sensors for Health Monitoring: A Qualitative Synthesis
Authors: Kristoffersson Annica , Lindén Maria
Published: March 2020

The use of wearable body sensors for health monitoring is a quickly growing field with the potential of offering a reliable means for clinical and remote health management. This includes both real-time monitoring and health trend monitoring with the aim to detect/predict health deterioration and also to act as a prevention tool. The aim of this systematic review was to provide a qualitative synthesis of studies using wearable body sensors for health monitoring. The synthesis and analysis have pointed out a number of shortcomings in prior research. Major shortcomings are demonstrated by the majority of the studies adopting an observational research design, too small sample sizes, poorly presented, and/or non-representative participant demographics (i.e., age, gender, patient/healthy). These aspects need to be considered in future research work.
Combining Pixel Swapping and Simulated Annealing for Land Cover Mapping
Authors: Su Lijuan , Xu Yue , Yuan Yan , Yang Jingyi
Published: March 2020

Mixed pixels commonly exist in low-resolution remote sensing images, and they are the key factors hindering the classification of land covers and high-precision mapping. To obtain the spatial information at the subpixel level, subpixel mapping (SPM) technologies, including the pixel-swapping algorithm (PSA), that use the unmixed proportions of various land covers and allocate subpixel land covers have been proposed. However, the PSA often falls into a local optimum solution. In this paper, we propose a SPM method, the PSA_MSA algorithm, that combines the PSA and the modified simulated annealing algorithm to find the global optimum solution. The modified simulated annealing algorithm swaps subpixels within a certain range to escape the local optimum solution. The method also optimizes all the mixed pixels in a randomized sequence to further improve the mapping accuracy. The experimental results demonstrate that the proposed PSA_MSA algorithm outperforms the existing PSA-based algorithms for SPM. The images with different spatial dependences are tested and the results show that the proposed algorithm is more suitable for images with high spatial autocorrelation. In addition, the effect of proportion error is analyzed by adding it in the experiments. The result shows that a higher proportion error rate leads to larger degradation of the subpixel mapping accuracy. Finally, the performance of PSA_MSA algorithm with different ranges of selection on subpixel-swapping is analyzed.