bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of Cholesterol and Myelin Basic Protein (MBP) Content on Lipid Monolayers Mimicking the Cytoplasmic Membrane of Myelin
Authors: Träger Jennica , Widder Katharina , Kerth Andreas , Harauz George , Hinderberger Dariush
Published: February 2020

Myelin basic protein (MBP) is located in the insulating covers of nerve cells in the brain and spinal cord. By interacting with lipid membranes, it is responsible for compaction of the myelin sheath in the central nervous system, which is weakened in demyelinating diseases. The lipid composition of the myelin leaflet has a high impact on the interaction between the membrane and MBP. Cholesterol is present in the cytoplasmic leaflet with a rather high amount of 44% (mol%). In this study, the focus is on the effect of cholesterol, mainly by varying its content, on the interaction of MBP with a lipid monolayer. Therefore, Langmuir lipid monolayers mimicking the cytoplasmic membrane of myelin and monolayers with variations of cholesterol content between 0% and 100% were measured at the air/water interface with additional imaging by fluorescence microscopy. All experiments were performed with and without bovine MBP to study the dependence of the interaction of the protein with the monolayers on the cholesterol content. The native amount of 44% cholesterol in the monolayer combines optima in the order of the monolayer (presumably correlating to compaction and thermodynamic stability) and protein interaction and shows unique features in comparison to lower or higher cholesterol contents.
Memory CD4+ T Cells in Immunity and Autoimmune Diseases
Authors: Raphael Itay , Joern Rachel R. , Forsthuber Thomas G.
Published: February 2020

CD4+ T helper (Th) cells play central roles in immunity in health and disease. While much is known about the effector function of Th cells in combating pathogens and promoting autoimmune diseases, the roles and biology of memory CD4+ Th cells are complex and less well understood. In human autoimmune diseases such as multiple sclerosis (MS), there is a critical need to better understand the function and biology of memory T cells. In this review article we summarize current concepts in the field of CD4+ T cell memory, including natural history, developmental pathways, subsets, and functions. Furthermore, we discuss advancements in the field of the newly-described CD4+ tissue-resident memory T cells and of CD4+ memory T cells in autoimmune diseases, two major areas of important unresolved questions in need of answering to advance new vaccine design and development of novel treatments for CD4+ T cell-mediated autoimmune diseases.
miRNA-Based Rapid Differentiation of Purified Neurons from hPSCs Advancestowards Quick Screening for Neuronal Disease Phenotypes In Vitro
Authors: Ishikawa Mitsuru , Aoyama Takeshi , Shibata Shoichiro , Sone Takefumi , Miyoshi Hiroyuki , Watanabe Hirotaka , Nakamura Mari , Morota Saori , Uchino Hiroyuki , Yoo Andrew S. , Okano Hideyuki
Published: February 2020

Obtaining differentiated cells with high physiological functions by an efficient, but simple and rapid differentiation method is crucial for modeling neuronal diseases in vitro using human pluripotent stem cells (hPSCs). Currently, methods involving the transient expression of one or a couple of transcription factors have been established as techniques for inducing neuronal differentiation in a rapid, single step. It has also been reported that microRNAs can function as reprogramming effectors for directly reprogramming human dermal fibroblasts to neurons. In this study, we tested the effect of adding neuronal microRNAs, miRNA-9/9*, and miR-124 (miR-9/9*-124), for the neuronal induction method of hPSCs using Tet-On-driven expression of the Neurogenin2 gene (Ngn2), a proneural factor. While it has been established that Ngn2 can facilitate differentiation from pluripotent stem cells into neurons with high purity due to its neurogenic effect, a long or indefinite time is required for neuronal maturation with Ngn2 misexpression alone. With the present method, the cells maintained a high neuronal differentiation rate while exhibiting increased gene expression of neuronal maturation markers, spontaneous calcium oscillation, and high electrical activity with network bursts as assessed by a multipoint electrode system. Moreover, when applying this method to iPSCs from Alzheimer’s disease (AD) patients with presenilin-1 (PS1) or presenilin-2 (PS2) mutations, cellular phenotypes such as increased amount of extracellular secretion of amyloid β42, abnormal oxygen consumption, and increased reactive oxygen species in the cells were observed in a shorter culture period than those previously reported. Therefore, it is strongly anticipated that the induction method combining Ngn2 and miR-9/9*-124 will enable more rapid and simple screening for various types of neuronal disease phenotypes and promote drug discovery.
Forward Current Transport Properties of AlGaN/GaN Schottky Diodes Prepared by Atomic Layer Deposition
Authors: Kim Hogyoung , Choi Seok , Choi Byung Joon
Published: February 2020

Atomic layer deposited AlGaN on GaN substrate with different thicknesses was prepared and the electron transport mechanism of AlGaN/GaN Schottky diodes was investigated. Above 348 K, both 5 and 10 nm thick AlGaN showed that the thermionic emission model with inhomogeneous Schottky barrier could explain the forward current transport. Analysis using a dislocation-related tunneling model showed that the current values for 10 nm thick AlGaN was matched well to the experimental data while those were not matched for 5 nm thick AlGaN. The higher density of surface (and interface) states was found for 5 nm thick AlGaN. In other words, a higher density of surface donors, as well as a thinner AlGaN layer for 5 nm thick AlGaN, enhanced the tunneling current.
Increasing the Efficiency of Dye-Sensitized Solar Cells by Adding Nickel Oxide Nanoparticles to Titanium Dioxide Working Electrodes
Authors: Tsai Chih-Hung , Lin Chia-Ming , Liu Yen-Cheng
Published: February 2020

In this study, nickel oxide (NiO) nanoparticles were added to a titanium dioxide (TiO2) nanoparticle paste to fabricate a dye-sensitized solar cell (DSSC) working electrode by using a screen-printing method. The effects of the NiO proportion in the TiO2 paste on the TiO2 working electrode, DSSC devices, and electron transport characteristics were comprehensively investigated. The results showed that adding NiO nanoparticles to the TiO2 working electrode both inhibited electron transport (a negative effect) and prevented electron recombination with the electrolyte (a positive effect). The electron transit time was extended following an increase in the amount of NiO nanoparticles added, confirming that NiO inhibited electron transport. Furthermore, the energy level difference between TiO2 and NiO generated a potential barrier that prevented the recombination of the electrons in the TiO2 conduction band with the I3- ions in the electrolyte. When the TiO2–NiO ratio was 99:1, the positive effects outweighed the negative effects. Therefore, this ratio was the optimal TiO2–NiO ratio in the electrode for electron transport. The DSSCs with a TiO2–NiO (99:1) working electrode exhibited an optimal power conversion efficiency of 8.39%, which was higher than the DSSCs with a TiO2 working electrode.
Influence of the Laser Wavelength on Harmful Effects on Granite Due to Biofilm Removal
Authors: Barreiro P. , Andreotti A. , Colombini M. P. , González P. , Pozo-Antonio J. S.
Published: February 2020

The colonization of stone-built monuments by different organisms (algae, fungi, lichens, bacteria, and cyanobacteria) can lead to biodeterioration of the stone, negatively affecting the artistic value of the heritage. To address this issue, laser cleaning has been widely investigated in recent years, due to the advantages it offers over traditional mechanical and chemical methods: it is gradual, selective, contactless, and environmentally friendly. That said, the laser parameters should be optimized in order to avoid any by-effects on the surface as a result of overcleaning. However, as the adjustment of each parameter to clean polymineralic stones is a difficult task, it would be useful to know the effect of overcleaning on the different forming minerals depending on the wavelength used. In this paper, three different wavelengths (355 nm, 532 nm, and 1064 nm) of a Q-Switch neodymium-doped yttrium aluminum garnet (Nd:Y3Al5O12) laser, commonly known as QS Nd:YAG laser were applied to extract a naturally developed sub-aerial biofilm from Vilachán granite, commonly used in monuments in the Northwest (NW)Iberian Peninsula. In addition to the removal rate of the biofilm, the by-effects induced for fluences higher than the damage threshold of the stone were evaluated using stereomicroscopy, color spectrophotometry, and scanning electron microscopy with energy-dispersive x-ray spectroscopy. The results showed that different removal rates were obtained depending on the wavelength used and 532 nm obtained the highest removal level. In terms of by-effects, biotite melting was registered on all surfaces regardless of the wavelength. In addition, 532 nm seemed to be the most aggressive laser system, inducing the greatest change in appearance as a result of extracting the kaolinite crackled coating and the segregations rich in Fe, which are a result of natural weathering. These changes were translated into colorimetric changes visible to the human eye. The surfaces treated with 355 nm and 1064 nm showed lower surface changes.
A Brief Review of Edible Coating Materials for the Microencapsulation of Probiotics
Authors: Pech-Canul Angel de la Cruz , Ortega David , García-Triana Antonio , González-Silva Napoleón , Solis-Oviedo Rosa Lidia
Published: February 2020

The consumption of probiotics has been associated with a wide range of health benefits for consumers. Products containing probiotics need to have effective delivery of the microorganisms for their consumption to translate into benefits to the consumer. In the last few years, the microencapsulation of probiotic microorganisms has gained interest as a method to improve the delivery of probiotics in the host as well as extending the shelf life of probiotic-containing products. The microencapsulation of probiotics presents several aspects to be considered, such as the type of probiotic microorganisms, the methods of encapsulation, and the coating materials. The aim of this review is to present an updated overview of the most recent and common coating materials used for the microencapsulation of probiotics, as well as the involved techniques and the results of research studies, providing a useful knowledge basis to identify challenges, opportunities, and future trends around coating materials involved in the probiotic microencapsulation.
Comparative Study between NiCoB and IrO2-Ta2O5/Ti Anodes for Application in Impressed Current Cathodic Protection (ICCP)
Authors: López Miguel Abraham , Pérez Quiroz José Trinidad , Ortega-Borges Raúl , Martínez Madrid Miguel , Rendón Belmonte Mariela , Salgado López Juan Manuel , Trejo Gabriel , Meas-Vong Yunny
Published: February 2020

In an impressed current cathodic protection system (ICCP) to protect structures against corrosion, the efficient operation depends on the proper selection of the electrodes, particularly the anode, chosen considering the structure to be protected and the environment in which it is located. The nature and overpotential of the anodic reaction determine the operation costs of an ICCP system so that proper anode selection is critically important for an ICCP system to function efficiently. Commercial anodes based on titanium substrates coated with iridium–tantalum oxide mixtures (IrO2-Ta2O5/Ti) are frequently used for this purpose due to low operating overpotentials. However, the gradual passivation of its surface limits its useful life and increases its operating costs, so it is necessary to seek competitive alternatives for its replacement. This study aimed to determine the feasibility of using carbon steel substrates coated with nickel/cobalt/boron (NiCoB/CS) as a viable low-cost alternative to replace IrO2-Ta2O5/Ti anodes in ICCP systems. Comparison between the electrochemical behavior and the corrosion resistance of both types of electrodes shows that the NiCoB/CS anode shows a good electrocatalytic activity and a higher corrosion resistance than IrO2-Ta2O5/Ti coated anodes, indicating that the NiCoB/CS anodes are promising low-cost candidates for ICCP systems.
Regium Bonds between Silver(I) Pyrazolates Dinuclear Complexes and Lewis Bases (N2, OH2, NCH, SH2, NH3, PH3, CO and CNH)
Authors: Alkorta Ibon , Trujillo Cristina , Sánchez-Sanz Goar , Elguero José
Published: February 2020

A theoretical study and Cambridge Structural Database (CSD) search of dinuclear Ag(I) pyrazolates interactions with Lewis bases were carried out and the effect of the substituents and ligands on the structure and on the aromaticity were analyzed. A relationship between the intramolecular Ag–Ag distance and stability was found in the unsubstituted system, which indicates a destabilization at longer distances compensated by ligands upon complexation. It was also observed that the asymmetrical interaction with phosphines as ligands increases the Ag–Ag distance. This increase is dramatically higher when two simultaneous PH3 ligands are taken into account. The calculated 109Ag chemical shielding shows variation up to 1200 ppm due to the complexation. Calculations showed that six-membered rings possessed non-aromatic character while pyrazole rings do not change their aromatic character significantly upon complexation.
Growth of Freestanding Gallium Nitride (GaN) Through Polyporous Interlayer Formed Directly During Successive Hydride Vapor Phase Epitaxy (HVPE) Process
Authors: Hu Haixiao , Zhang Baoguo , Liu Lei , Xu Deqin , Shao Yongliang , Wu Yongzhong , Hao Xiaopeng
Published: February 2020

The progress of nitride technology is widely limited and hindered by the lack of high-quality gallium nitride (GaN) wafers. Therefore, a large number of GaN epitaxial devices are grown on heterogeneous substrates. Although various additional treatments of substrate have been used to promote crystal quality, there is still plenty of room for its improvement, in terms of direct and continuous growth based on the hydride vapor phase epitaxy (HVPE) technique. Here, we report a three-step process that can be used to enhance the quality of GaN crystal by tuning V/III rate during successive HVPE process. In the growth, a metal-organic chemical vapor deposition (MOCVD) grown GaN on sapphire (MOCVD-GaN/Al2O3) was employed as substrate, and a high-quality GaN polyporous interlayer, with successful acquisition, without any additional substrate treatment, caused the growth stress to decrease to 0.06 GPa. Meanwhile the quality of GaN improved, and the freestanding GaN was directly obtained during the growth process.
Enhanced Heat Transfer in Moderately Ionized Liquid Due to Hybrid MoS2/SiO2 Nanofluids Exposed by Nonlinear Radiation: Stability Analysis
Authors: Khan Umair , Zaib A. , Khan Ilyas , Baleanu Dumitru , Nisar Kottakkaran Sooppy
Published: February 2020

This study considers ethylene-glycol as a moderate ionized regular liquid whose rheological behavior can be analyzed through the relations of the Carreau stress–strain tensor. Hybrid nanoliquids are potent liquids that give better performance for heat transfer and the properties of thermo physical than regular heat transfer liquids (water, ethylene glycol, and oil) and nanoliquids by single nanomaterials. Here, a type of hybrid nanoliquid involving silicon oxide (SiO2) and Molybdenum disulfide (MoS2) nanoparticles with ethylene glycol as a base liquid are considered. In addition, the impact of nonlinear radiation along with Lorentz force is invoked. Similarity variables are utilized to acquire the numerical findings and their solutions for transmuting ordinary differential equations (ODEs). Using bvp4c from MATLAB, we can obtain these quantitative and numerical results of the converted nonlinear equations. The impacts of the pertinent constraints on the temperature distribution, velocity, Nusselt number, and skin friction are estimated. The outcomes indicate that the double-edged methods for the results originate from the precise values of the permeable parameters. Further, the critical values (Sc = 1.9699, 2.0700 and 2.2370) are enhanced due to the influence of the local Weissenberg number. This implies that the increasing value of the local Weissenberg number accelerate the boundary layer separation. Furthermore, a stability investigation is performed and confirms that the first solution is a physically reliable solution.
Measuring and Linking the Missing Part of Biodiversity and Ecosystem Function: The Diversity of Biotic Interactions
Authors: Luna Pedro , Corro Erick J. , Antoniazzi Reuber , Dáttilo Wesley
Published: February 2020

Biotic interactions are part of all ecosystem attributes and play an important role in the structure and stability of biological communities. In this study, we give a brief account of how the threads of biotic interactions are linked and how we can measure such complexity by focusing on mutualistic interactions. We start by explaining that although biotic interactions are fundamental ecological processes, they are also a component of biodiversity with a clear α, β and γ diversity structure which can be measured and used to explain how biotic interactions vary over time and space. Specifically, we explain how to estimate the α-diversity by measuring the properties of species interaction networks. We also untangle the components of the β-diversity and how it can be used to make pairwise comparisons between networks. Moreover, we move forward to explain how local ecological networks are a subset of a regional pool of species and potential interactions, γ-diversity, and how this approach allows assessing the spatial and temporal dynamics of ecological networks. Finally, we propose a new framework for studying interactions and the biodiversity–ecosystem function relationship by identifying the unique and common interactions of local networks over space, time or both together.
A Novel Multi-Criteria Decision-Making Model for Building Material Supplier Selection Based on Entropy-AHP Weighted TOPSIS
Authors: Chen Chun-Ho
Published: February 2020

The type of criterion weight can be distinguished according to different decision methods. Subjective weights are given by decision makers based on their knowledge, experience, expertise, and other factors. Objective weights are obtained through multi-step calculations of the evaluation matrix constructed from the actual information about the evaluation criteria of the alternatives. A single consideration of these two types of weights often results in biased results. In addition, in order to build an effective supply chain source, buyers must find suitable quality products and/or service providers in the process of supplier selection. Based on the above reasons, it is difficult to accurately select the appropriate alternative. The main contribution of this paper is to combine entropy weight, analytic hierarchy process (AHP) weight, and the technique for order preference by similarity to an ideal solution (TOPSIS) method into a suitable multi-criteria decision making (MCDM) solution. The TOPSIS method is extended with entropy-AHP weights, and entropy-AHP weights are used instead of subjective weights. A novel decision-making model of TOPSIS integrated entropy-AHP weights is proposed to select the appropriate supplier. Finally, we take the selection of building material suppliers as an example and use sensitivity analysis to show that the combination of the TOPSIS method based on entropy-AHP weights can effectively select the appropriate supplier.
A Climate-Economy Policy Model for Barbados
Authors: Kemp-Benedict Eric , Drakes Crystal , Canales Nella
Published: February 2020

Small island developing states (SIDS), such as Barbados, must continually adapt in the face of uncertain external drivers. These include demand for exports, tourism demand, and extreme weather events. Climate change introduces further uncertainty into the external drivers. To address the challenge, we present a policy-oriented simulation model that builds upon prior work by the authors and their collaborators. Intended for policy analysis, it follows a robust decision making (RDM) philosophy of identifying policies that lead to positive outcomes across a wide range of external changes. While the model can benefit from further development, it illustrates the importance for SIDS of incorporating climate change into national planning. Even without climate change, normal variation in export and tourism demand drive divergent trajectories for the economy and external debt. With climate change, increasing storm damage adds to external debt as the loss of productive capital and need to rebuild drives imports.
Task-Agnostic Object Recognition for Mobile Robots through Few-Shot Image Matching
Authors: Chiatti Agnese , Bardaro Gianluca , Bastianelli Emanuele , Tiddi Ilaria , Mitra Prasenjit , Motta Enrico
Published: February 2020

To assist humans with their daily tasks, mobile robots are expected to navigate complex and dynamic environments, presenting unpredictable combinations of known and unknown objects. Most state-of-the-art object recognition methods are unsuitable for this scenario because they require that: (i) all target object classes are known beforehand, and (ii) a vast number of training examples is provided for each class. This evidence calls for novel methods to handle unknown object classes, for which fewer images are initially available (few-shot recognition). One way of tackling the problem is learning how to match novel objects to their most similar supporting example. Here, we compare different (shallow and deep) approaches to few-shot image matching on a novel data set, consisting of 2D views of common object types drawn from a combination of ShapeNet and Google. First, we assess if the similarity of objects learned from a combination of ShapeNet and Google can scale up to new object classes, i.e., categories unseen at training time. Furthermore, we show how normalising the learned embeddings can impact the generalisation abilities of the tested methods, in the context of two novel configurations: (i) where the weights of a Convolutional two-branch Network are imprinted and (ii) where the embeddings of a Convolutional Siamese Network are L2-normalised.
Language Cognition and Pronunciation Training Using Applications
Authors: Kan Ming Sung , Ito Atsushi
Published: February 2020

In language learning, adults seem to be superior in their ability to memorize knowledge of new languages and have better learning strategies, experiences, and intelligence to be able to integrate new knowledge. However, unless one learns pronunciation in childhood, it is almost impossible to reach a native-level accent. In this research, we take the difficulties of learning tonal pronunciation in Mandarin as an example and analyze the difficulties of tone learning and the deficiencies of general learning methods using the cognitive load theory. With the tasks designed commensurate with the learner’s perception ability based on perception experiments and small-step learning, the perception training app is more effective for improving the tone pronunciation ability compared to existing apps with voice analysis function. Furthermore, the learning effect was greatly improved by optimizing the app interface and operation procedures. However, as a result of the combination of pronunciation practice and perception training, pronunciation practice with insufficient feedback could lead to pronunciation errors. Therefore, we also studied pronunciation practice using machine learning and aimed to train the model for the pronunciation task design instead of classification. We used voices designed as training data and trained a model for pronunciation training, and demonstrated that supporting pronunciation practice with machine learning is practicable.
Antioxidant and Anti-Inflammatory Properties of Cherry Extract: Nanosystems-Based Strategies to Improve Endothelial Function and Intestinal Absorption
Authors: Beconcini Denise , Felice Francesca , Fabiano Angela , Sarmento Bruno , Zambito Ylenia , Di Stefano Rossella
Published: February 2020

Cherry fruit has a high content in flavonoids. These are important diet components protecting against oxidative stress, inflammation, and endothelial dysfunction, which are all involved in the pathogenesis of atherosclerosis, which is the major cause of cardiovascular diseases (CVD). Since the seasonal availability of fresh fruit is limited, research has been focused on cherry extract (CE), which also possesses a high nutraceutical potential. Many clinical studies have demonstrated the nutraceutical efficacy of fresh cherries, but only a few studies on CE antioxidant and anti-inflammatory activities have been carried out. Here, the results concerning the antioxidant and anti-inflammatory activities of CE are reviewed. These were obtained by an in vitro model based on Human Umbilical Vein Endothelial Cells (HUVEC). To clarify the CE mechanism of action, cells were stressed to induce inflammation and endothelial dysfunction. Considering that antioxidants’ polyphenol compounds are easily degraded in the gastrointestinal tract, recent strategies to reduce the degradation and improve the bioavailability of CE are also presented and discussed. In particular, we report on results obtained with nanoparticles (NP) based on chitosan derivatives (Ch-der), which improved the mucoadhesive properties of the chitosan polymers, as well as their positive charge, to favor high cellular interaction and polyphenols intestinal absorption, compared with a non-mucoadhesive negative surface charged poly(lactic-co-glycolic) acid NP. The advantages and safety of different nanosystems loaded with natural CE or other nutraceuticals are also discussed.
Sensory Analysis in Assessing the Possibility of Using Ethanol Extracts of Spices to Develop New Meat Products
Authors: Szymandera-Buszka Krystyna , Waszkowiak Katarzyna , Jędrusek-Golińska Anna , Hęś Marzanna
Published: February 2020

The food industry has endeavoured to move toward the direction of clean labelling. Therefore, replacing synthetic preservatives with natural plant extracts has gained significant importance. It is necessary to determine whether products enriched with such extracts are still accepted by consumers. In this study, consumer tests (n = 246) and sensory profiling were used to assess the impact of ethanol extracts of spices (lovage, marjoram, thyme, oregano, rosemary, and basil; concentration 0.05%) on the sensory quality of pork meatballs and hamburgers. The desirability of meat products with spice extracts to consumers depended on the added extract. The highest scores were for products with lovage extract, whose sensory profile was the most similar to the control sample without the addition of an extract (with higher intensity of broth taste compared with the others). Products with rosemary and thyme extracts were characterised by lower desirability than the control. This was related to the high intensity of spicy and essential oil tastes, as well as the bitter taste in the case of products with thyme. The studied extracts of spices allow for the creation of meat products (meatballs and hamburgers) with high consumer desirability, however, the high intensity of essential oil and spicy tastes might be a limitation.
Quantification of Cheese Yield Reduction in Manufacturing Parmigiano Reggiano from Milk with Non-Compliant Somatic Cells Count
Authors: Franceschi Piero , Faccia Michele , Malacarne Massimo , Formaggioni Paolo , Summer Andrea
Published: February 2020

The mammary gland inflammation process is responsible for an increased number of somatic cells in milk, and transfers into the milk of some blood components; this causes alterations in the chemical composition and physico-chemical properties of milk. For this reason, somatic cell count (SCC) is one of the most important parameters of milk quality; therefore, European Union (EU) Regulation no 853/2004 has stated that it must not exceed the limit value of 400,000 cells/mL. The research aimed to compare chemical composition, cheese yield, and cheesemaking losses of two groups of vat milks used for Parmigiano Reggiano production, characterized by different SCC levels. During two years, ten cheesemaking trials were performed in ten different cheese factories. In each trial, two cheesemaking processes were conducted in parallel: one with low SCC milk (below 400,000 cells/mL; Low Cell Count (LCC)) and the other with high SCC milk (400,000–1,000,000 cells/mL; High Cell Count (HCC)). For each trial, vat milk and cooked whey were analyzed; after 24 months of ripening, cheeses were weighed to calculate cheese yield. The HCC group had lower casein content (2.43 vs. 2.57 g/100 g; p ≤ 0.05) and number (77.03% vs. 77.80%; p ≤ 0.05), lower phosphorus (88.37 vs. 92.46 mg/100g; p ≤ 0.05) and titratable acidity (3.16 vs. 3.34 °SH/50 mL; p ≤ 0.05) compared to LCC. However, chloride (111.88 vs. 104.12 mg/100 g; p ≤ 0.05) and pH (6.77 vs. 6.71; p ≤ 0.05) were higher. Fat losses during cheesemaking were higher (20.16 vs. 16.13%). After 24 months of ripening, cheese yield was 8.79% lower for HCC milk than LCC (6.74 vs. 7.39 kg/100 kg; p ≤ 0.05).
Assessment of Water Mobility in Surf Clam and Soy Protein System during Gelation Using LF-NMR Technique
Authors: Wang Siqi , Lin Rong , Cheng Shasha , Wang Zhixiang , Tan Mingqian
Published: February 2020

Water mobility and distribution of a dual-protein system of surf clam myofibrillar protein (MP) and soy protein (SP) was investigated by the nondestructive low field nuclear magnetic resonance (LF-NMR) technique. Four proton populations were found in the contour plots of T2 relaxation times for the SP-MP system. The first component, (T21), was assigned to the highly integrated water located in protein macromolecules with a relaxation time of approximately 1.15 ms. The second signal, T22, with a relaxation time of 2.20 to 38.00 ms was regarded as the inter-myofibrillar water trapped in organized protein structures. The third component, T23, with a relaxation time of around 100 ms was ascribed to the extra-myofibrillar water. With an increase in temperature, T24 appeared which was assigned to the free water within the extra-myofibrillar space. The gelation behavior occurred at 70, 62, and 52 °C as the proportion of SP/MP was 4:6, 2:8, and 0:10, respectively. The principal component analysis (PCA) and heatmap of LF-NMR data analysis showed potential for distinguishing the different dual-protein systems formed at various temperatures. The analysis of storage modulus G′, loss modulus G″, and tanδ confirmed the change trend of the LF-NMR results. The measurements of cooking loss, water holding capability, and gel strength further revealed that the SP and MP were likely to form a gel network with an increase of additional clam protein. The hydrophobicity analysis showed, for the systems with the SP/MP proportions of 4:6, 2:8, and 0:10, more hydrophobic groups were exposed when the temperature was over 50 °C. Scanning electron microscopy showed that the number of the micropores increased with an addition of MP in the dual-protein system of SP/MP. All the results demonstrated that LF-NMR has great potential for characterizing the gelation process of a dual-protein system.
Effects of Nonstarch Genetic Modifications on Starch Structure and Properties
Authors: Yu Shiyao , Du Dengxiang , Wu Alex C. , Bai Yeming , Wu Peng , Li Cheng , Gilbert Robert G.
Published: February 2020

This paper examines if, in maize, starch structure and starch-dependent properties might be altered by pleiotropic effects arising from genetic modifications that are not directly related to starch synthesis. The molecular structure, specifically the starch chain-length distributions (CLDs), of two maize lines transformed with Bar (bialaphos resistance) and Cry1c genes (an artificial gene, encoding proteinaceous insecticidal δ-endotoxins) were compared to those of their control lines. The two transgenes are responsible for herbicidal resistance and insect tolerance, respectively. The starch CLDs were measured by enzymatic debranching and measuring the molecular weight distributions of the resulting linear chains. It was found that although all the lines had similar amylose contents, the CLDs of both amylopectin and amylose for Cry1c were noticeably different from the others, having more short amylopectin and long amylose chains. These CLDs are known to affect functional properties, and indeed it was found that the Cry1c transgenic lines showed a lower gelatinization temperature and faster digestion rate than the control or Bar lines. However, a slower digestion rate is nutritionally desirable. Thus, pleiotropic effects from genetic modifications can indirectly but significantly affect the starch synthesis pathway and thus change functional properties of significance for human health.
Insights into the Identification of the Specific Spoilage Organisms in Chicken Meat
Authors: Saenz-García Cinthia E. , Castañeda-Serrano Pilar , Mercado Silva Edmundo M. , Alvarado Christine Z. , Nava Gerardo M.
Published: February 2020

Poultry meat deterioration is caused by environmental conditions, as well as proliferation of different bacterial groups, and their interactions. It has been proposed that meat spoilage involves two bacterial groups: one group that initiates the deterioration process, known as specific spoilage organisms (SSOs), and the other known as spoilage associated organisms (SAOs) which represents all bacteria groups recovered from meat samples before, during, and after the spoilage process. Numerous studies have characterized the diversity of chicken meat SAOs; nonetheless, the identification of the SSOs remains a long-standing question. Based on recent genomic studies, it is suggested that the SSOs should possess an extensive genome size to survive and proliferate in raw meat, a cold, complex, and hostile environment. To evaluate this hypothesis, we performed comparative genomic analyses in members of the meat microbiota to identify microorganisms with extensive genome size and ability to cause chicken meat spoilage. Our studies show that members of the Pseudomonadaceae family have evolved numerous biological features such as large genomic size, slow-growing potential, low 16S rRNA copy number, psychrotrophic, and oligotrophic metabolism to initiate the spoilage of poultry meat. Moreover, inoculation experiments corroborated that these biological traits are associated with the potential to cause chicken meat deterioration. Together, these results provide new insights into the identification of SSO. Further studies are in progress to elucidate the impact of the SSO on meat quality and microbiota diversity.
Identification of Key Off-Flavor Compounds in Thermally Treated Watermelon Juice via Gas Chromatography–Olfactometry–Mass Spectrometry, Aroma Recombination, and Omission Experiments
Authors: Yang Xiao , Yang Fan , Liu Ye , Li Jian , Song Huan-Lu
Published: February 2020

Thermally treated watermelon juice (TW) presents a strong unpleasant smell, resulting in poor consumer acceptance. It is necessary to identify the key off-flavor compounds in TW. Solid-phase microextraction (SPME) and solvent-assisted flavor evaporation (SAFE) coupled with gas chromatography–olfactometry–mass spectrometry (GC–O–MS) were applied to the extraction and analysis of the volatile compounds in TW. Five aroma-active compounds and seven off-flavor compounds were quantitatively analyzed by the standard curve method. Based on the flavor dilution factor (FD), odor attribute, odor activity value (OAV) of volatile compounds, and partial least-squares regression (PLSR) analysis, seven key off-flavor compounds were preliminarily identified as follows: (E)-2-heptenal, decanal, octanol, diisopropyl disulfide, hexanol, (E)-2-decenal, and (E)-2-octenol. Aroma recombination proved that these off-flavor compounds above had a negative impact on the overall flavor in TW. Omission experiments were taken to confirm them further. Finally, octanol, diisopropyl disulfide, and (E)-2-decenal were identified as the most potent off-flavor compounds in TW.
The Snacking Chameleon: Psychological Proximity Increases Imitation of Food Intake Independently of Brand Choice
Authors: Bischoff Claudia , Reutner Leonie , Hansen Jochim
Published: February 2020

Observing other people snacking can affect one’s own consumption behavior. The present experiment tested whether temporal distance moderates imitation of brand choice and the number of snacks consumed. Based on previous research demonstrating that psychological distance (e.g., temporal or spatial distance) reduces imitation of movements, we hypothesized that participants would imitate the amount of food intake to a lesser degree when they temporally distance themselves from a model person. To test this idea, participants (n = 113) were asked to imagine their life either the next day (proximal condition) or in one year (distant condition). Next, participants watched a video clip depicting a model person who chose one of two brands of pretzels and ate either plenty or just a few of the pretzels. Then, participants chose one of the two brands of pretzels, served themselves as many of the pretzels as they liked, and ate them while filling in a tasting questionnaire. As expected, participants primed with proximity imitated snack intake more than participants primed with distance. The brand choice was not affected by self-distancing. Implications for snacking behavior are discussed.
Techno-Functional, Nutritional and Environmental Performance of Protein Isolates from Blue Lupin and White Lupin
Authors: Vogelsang-O’Dwyer Martin , Bez Juergen , Petersen Iben Lykke , Joehnke Marcel Skejovic , Detzel Andreas , Busch Mirjam , Krueger Martina , Ispiryan Lilit , O’Mahony James A. , Arendt Elke K. , Zannini Emanuele
Published: February 2020

Similarly prepared protein isolates from blue lupin (Lupinus angustifolius) and white lupin (L. albus) were assessed in relation to their composition, functional properties, nutritional attributes and environmental impacts. Blue lupin protein isolate (BLPI) and white lupin protein isolate (WLPI) were found to be quite similar in composition, although differences in the electrophoretic protein profiles were apparent. Both lupin protein isolates (LPIs) had good protein solubility (76.9% for BLPI and 69.8% for WLPI at pH 7) and foaming properties. However, a remarkable difference in heat gelation performance was observed between BLPI and WLPI. WLPI had a minimum gelling concentration of 7% protein, whereas BLPI required 23% protein in order to form a gel. WLPI also resulted in stronger gels over a range of concentrations compared to BLPI. Nutritional properties of both LPIs were similar, with no significant differences in in vitro protein digestibility (IVPD), and both had very low trypsin inhibitor activity (TIA) and fermentable oligo-, di- and monosaccharides, and polyols (FODMAP) content. The amino acid profiles of both LPIs were also similar, with sulfur-containing amino acids (SAAs) being the limiting amino acid in each case. Environmental impacts revealed by the life cycle assessment (LCA) were almost identical for BLPI and WLPI, and in most categories the LPIs demonstrated considerably better performance per kg protein when compared to cow’s whole milk powder.
Effect of Glazing with Different Materials on the Quality of Tuna During Frozen Storage
Authors: Wang Jinfeng , Yu Wenhui , Xie Jing
Published: February 2020

This study investigated and determined the changes in various qualities of tuna samples that were glazed with rosmarinic acid, a bamboo leaf antioxidant, and sodium lactate and stored at −18 °C for 180 days. The water-holding capacity, cooking loss, color, texture, protein content, and total volatile basic nitrogen (TVB-N) were monitored, to study the effect of tuna glazed with different materials on the quality every 30 days. Low-field nuclear magnetic resonance (LF-NMR) was used to measure the water distribution of tuna in this paper. The results showed that the quality of unglazed tuna decreased significantly after 180 days of frozen storage. During frozen storage, the hardness and a* values of RG (glazed with the rosmarinic acid group), SG (glazed with the sodium lactate group), and CG (glazed with the composite of rosmarinic acid, sodium lactate, and the antioxidant of bamboo leaf) tuna decreased slowly, while the malondialdehyde (MDA) value of AG (glazed with the antioxidant of bamboo leaf group) tuna increased slowly. After 180 days of frozen storage, CG tuna had the highest protein content and the lowest TVB-N value, which may have been due to the synergistic effect of glazing materials. The tuna with CG also had the best freshness and quality after frozen storage. Considering the results, a composite of rosemary (0.3%), sodium lactate (3.4%), and antioxidants of bamboo leaves (0.12%) is the best material for glazing tuna.
Quantification and Distribution of Omega-3 Fatty Acids in South Pacific Fish and Shellfish Species
Authors: Rincón-Cervera Miguel Ángel , González-Barriga Valeria , Romero Jaime , Rojas Rodrigo , López-Arana Sandra
Published: February 2020

Fatty acid composition and distribution in edible species of fish and shellfish captured in the South Pacific were studied, with a focus on n-3 long-chain polyunsaturated fatty acids (n-3 LCPUFA). Fatty acids were quantified using gas-chromatography coupled with flame ionization detection (GC-FID), and the distribution of different fatty acids within lipid classes (neutral and polar lipids) was achieved after oil extraction using the Folch method and separation of lipid classes via solid-phase extraction for further GC-FID analysis. Red cusk-eel was the fish species with the lowest content of both EPA and DHA (40.8 and 74.4 mg/100 g, respectively) whereas mackerel contained the highest amount (414.7 and 956.0 mg/100 g for EPA and DHA, respectively). Sea squirt was the shellfish species with the highest content of EPA and DHA (375.0 and 165.7 mg/100 g, respectively) whereas the lowest amount of EPA + DHA was found in Chilean abalone (63.6 mg/100 g). PUFA were mostly found in neutral or polar lipids depending on the studied species. Indexes used to discuss the nutritional quality of lipids (PUFA/SFA, n-6/n-3 ratio and the hypocholesterolemic/hypercholesterolemic fatty acid index) were calculated and reported in the manuscript. This information provides a novel nutritional insight which may be useful to help nutritionists and other health professionals give more accurate counseling for the population to reach the recommended daily intakes of EPA and DHA.
Combination of Low Fluctuation of Temperature with TiO2 Photocatalytic/Ozone for the Quality Maintenance of Postharvest Peach
Authors: Jia Xiaoyu , Li Jiangkuo , Du Meijun , Zhao Zhiyong , Song Jianxin , Yang Weiqiao , Zheng Yanli , Chen Lan , Li Xihong
Published: February 2020

Chilling injury, tissue browning, and fungal infection are the major problems of peach fruit during post-harvest storage. In this study, a precise temperature control cold storage with low-temperature fluctuation (LFT) and internal circulation flow system is designed. An ozone (O3) generator and a (titanium dioxide) TiO2 photocatalytic reactor were applied to cold storage to investigate the variation of LFT combined with ozone fumigation and a TiO2 photocatalytic reactor in the efficiency of delaying ripening and maintaining peach fruit quality. Results showed that the temperature fluctuation with the improved control system was only ±0.1 to ±0.2 °C compared with that of ±0.5 to ±1.0 °C in conventional cold storage. LFT significantly reduced the chilling injury of peach fruit during storage. Although LFT combined with fumigation of 200 mg m−3 ozone periodical treatment slightly damaged the peach fruit after 40 d of storage, its combination with the TiO2 photocatalytic system significantly improved the postharvest storage quality of the fruit. This treatment maintained higher titratable acidity (TA), total soluble solids (TSS), better firmness, color, microstructure, and lower decay rate, polyphenol oxidase (PPO) activities, total phenol accumulation, respiratory intensity, ethylene production, and malondialdehyde (MDA) content during 60 d of storage. All the results show that LFT combined with the TiO2 photocatalytic system might be a promising technology for quality preservation in peach fruit storage.
Determination of the Volatile Profile of Lemon Peel Oils as Affected by Rootstock
Authors: Aguilar-Hernández Marlene G. , Sánchez-Bravo Paola , Hernández Francisca , Carbonell-Barrachina Ángel A. , Pastor-Pérez Joaquín J. , Legua Pilar
Published: February 2020

Citrus limon (L.) Burm is an important crop that grows between latitudes 30° North and 30° South, the main producers being China, the USA, Mexico, India, Brazil, and Spain. In Spain, lemon grows mainly in Mediterranean areas such as Murcia, Valencia, and Andalucía. The most cultivated varieties are “Fino” and “Verna”. In this study, five varieties of lemon, “Verna”, “Bétera”, “Eureka”, “Fino 49”, and “Fino 95” were evaluated on different rootstocks: three new Forner-Alcaide (“FA13”, “FA5”, “FA517”), Citrus macrophylla, Wester, and Citrus aurantium L. Hydrodistillation was used to obtain essential oil from fresh peels and then the volatile profile was studied by gas chromatography-mass spectrometry (GC-MS). A total of 26 volatile compounds were identified, limonene being the main one followed by β-pinene, γ-terpinene, sabinene, and α-pinene. The results revealed that Forner-Alcaide rootstocks (“FA5” > “FA517” > “FA13”) proved to be the best rootstocks for the aroma quality as they led to high volatile contents, followed by C. aurantium and C. macrophylla. Among the other varieties, the most aromatic one was “Eureka”. The whole trend was as follows (in decreasing order): “Eureka” > “Bétera” > “Fino 95” > “Verna” > “Fino 49”.
Pathogenic Mitochondria DNA Mutations: Current Detection Tools and Interventions
Authors: Mustafa Mohd Fazirul , Fakurazi Sharida , Abdullah Maizaton Atmadini , Maniam Sandra
Published: February 2020

Mitochondria are best known for their role in energy production, and they are the only mammalian organelles that contain their own genomes. The mitochondrial genome mutation rate is reported to be 10–17 times higher compared to nuclear genomes as a result of oxidative damage caused by reactive oxygen species during oxidative phosphorylation. Pathogenic mitochondrial DNA mutations result in mitochondrial DNA disorders, which are among the most common inherited human diseases. Interventions of mitochondrial DNA disorders involve either the transfer of viable isolated mitochondria to recipient cells or genetically modifying the mitochondrial genome to improve therapeutic outcome. This review outlines the common mitochondrial DNA disorders and the key advances in the past decade necessary to improve the current knowledge on mitochondrial disease intervention. Although it is now 31 years since the first description of patients with pathogenic mitochondrial DNA was reported, the treatment for mitochondrial disease is often inadequate and mostly palliative. Advancements in diagnostic technology improved the molecular diagnosis of previously unresolved cases, and they provide new insight into the pathogenesis and genetic changes in mitochondrial DNA diseases.