bluuebunny/crossref_metadata_2025 huggingface dataset:
Calcium and Strontium Coordination Polymers as Controlled Delivery Systems of the Anti-Osteoporosis Drug Risedronate and the Augmenting Effect of Solubilizers
Authors: Vassaki Maria , Kotoula Christina , Turhanen Petri , Choquesillo-Lazarte Duane , Demadis Konstantinos D.
Published: December 2021

Bisphosphonates (BPs) constitute a class of drugs used for the treatment of calcium- and bone-related disorders, including osteoporosis, Paget’s disease, etc. The presence of the anionic phosphonate groups endows them with the ability to act as ligands to metal ions. As a result, the synthesis of complexes or coordination polymers of various structural motifs can be accomplished. In this work, the 3rd generation BP drug risedronate (RIS) was combined with biologically acceptable alkaline earth metal ions (e.g., Ca2+ and Sr2+) in an effort to synthesize new materials. These metal–RIS compounds can operate as controlled delivery systems (CDSs) when exposed to appropriate experimental conditions, such as the low pH of the human stomach. CDS networks containing Ca2+ or Sr2+ and RIS were physicochemically and structurally characterized and were evaluated for their ability to release the free RIS drug during an acid-driven hydrolysis process. Due to the low solubility of RIS at low pH, cationic additives (linear polyethyleneimine and amine-terminated polyaminoamide dendrimer) were utilized as drug solubilizers. Based on the drug release results of this study, there was an attempt to correlate the drug release efficiency with the structural features of these CDSs.
Influence of Probiotic Supplementation on Health Status of the Dogs: A Review
Authors: Sivamaruthi Bhagavathi Sundaram , Kesika Periyanaina , Chaiyasut Chaiyavat
Published: December 2021

Most commonly, pet dogs suffer from gastrointestinal (GI) diseases due to careless eating behaviors, such as eating food other than dog food; excess or insufficient nutrient intake of food leading to malnutrition, which could be harmful to dogs; a lack of digestive enzymes; food intolerance or allergies; infections; and/or breed-related hypersensitivities. Probiotics are live microorganisms that deliver health benefits to the host when administrated in an adequate amount. The possible mechanism behind probiotics’ beneficial effects could be their positive regulation of the host’s intestinal microbiota. Probiotics are reported to have therapeutic properties against canine GI and other diseases. The most suitable dosages and applications of probiotics have not been evaluated extensively. The present review summarizes current knowledge regarding the benefits of probiotics and the changes in canine microbiota during probiotic interventions. This literature review provides clinical evidence for probiotics’ beneficial effects in preventing or treating canine ill-health conditions. Based on current knowledge, subsequent researchers could develop or improve probiotics-based canine pharmacological products.
Dissolution of Biofilm Secreted by Three Different Strains of Pseudomonas aeruginosa with Bromelain, N-Acetylcysteine, and Their Combinations
Authors: Carter Carly J. , Pillai Krishna , Badar Samina , Mekkawy Ahmed H. , Akhter Javed , Jefferies Thomas , Valle Sarah J. , Morris David L.
Published: December 2021

Bacterial infection of hernia mesh with the formation of biofilms presents a barrier to antibiotic treatment with subsequent surgical intervention and hospitalization. Hence, in the current study, we examined the effect of BromAc, a mucolytic agent, on the dissolution of biofilm formed by three different strains of Pseudomonas aeruginosa. Pseudomonas aeruginosa was carefully grown on hernia mesh and treated with various concentrations of bromelain, NAC, and their combinations at 37 °C over 4 h in vitro. Then, the biofilm dissolution activities of the agents were evaluated. Moreover, the combination index (CI) was analyzed to determine the synergy of the bromelain and NAC combination. The results indicated that biofilms were more susceptible to degradation by bromelain, whilst NAC showed growth enhancement in two of the strains. However, in combination (BromAc), the three strains were dramatically affected by the agents, with more than 80% debridement fir a suitable combination of bromelain and NAC that was also strain-specific. Hence, the current study shows that the biofilms formed by these three strains of Pseudomonas aeruginosa were adversely affected by a single treatment of BromAc, with more than 80% debridement, indicating that subsequent treatment may abolish the biofilm completely.
Particulate Matter Contamination of Bee Pollen in an Industrial Area of the Po Valley (Italy)
Authors: Papa Giulia , Capitani Giancarlo , Pellecchia Marco , Negri Ilaria
Published: December 2021

The global demand for bee pollen as a dietary supplement for human nutrition is increasing. Pollen, which comprises proteins and lipids from bees’ diets, is rich in essential amino acids, omega fatty acids, and bioactive compounds that can have beneficial effects on human health. However, bee pollen may also contain contaminants due to environmental contamination. To date, data on bee pollen contamination by environmental pollutants refer almost exclusively to pesticides and heavy metals, and very little information is available on the potential contamination of bee pollen by airborne particulate matter (PM), a ubiquitous pollutant that originates from a wide range of anthropogenic sources (e.g., motor vehicles, industrial processes, agricultural operations). In the present study, pollen grains collected by forager bees living in an industrial area of the Po Valley (Northern Italy) were analyzed for contamination by inorganic PM. The morpho-chemical characterization of inorganic particles using SEM/EDX allowed us to identify different emission sources and demonstrate the potential risk of PM entering the food chain and exposing bees to its ingestion.
M13 Phages Uptake of Gold Nanoparticles for Radio- and Thermal-Therapy and Contrast Imaging Improvement
Authors: Torrisi Lorenzo , Silipigni Letteria , Kovacik Lubomir , Lavrentiev Vasily , Cutroneo Mariapompea , Torrisi Alfio , De Plano Laura , Franco Domenico , Guglielmino Salvatore
Published: December 2021

The presented work deals with the uptake of gold nanoparticles (Au NPs) by M13 phages in solutions. In particular, the Au NPs uptake modalities and their localization in the filamentous phages are evaluated and measured. Gold spherical nanoparticles (with an average diameter of the order of 10 nm) are obtained by laser ablation in water with a sodium citrated surfactant. The interest of such application comes from the possibility to employ living biological structures to transport heavy metallic nanoparticles inside cells of tumoral tissues. Indeed, phages have the capability to introduce Au NPs in the proximity to the cell nucleus, increasing the efficiency of DNA destruction in the tumoral cells by employing low doses of ionizing radiation during radiotherapy and hyperthermia treatments. Several analyses and microscopy characterizations of the prepared phages samples embedding gold nanoparticles are presented, demonstrating that the presence of Au NPs increases the phages imaging contrast.
Biosynthesis of Zinc Oxide Nanoparticles Using Leaf Extract of Prosopis juliflora as Potential Photocatalyst for the Treatment of Paper Mill Effluent
Authors: Abbas Ahmed , Hammad Sabah , Sallam Heba , Mahfouz Lamiaa , Ahmed Mohamed , Abboudy Sayed , Ahmed Ahmed , Alhag Sadeq , Taher Mostafa , Alrumman Sulaiman , Alshehri Mohammed , Soliman Wagdi , Tabassum-Abbasi  , Mostafa Massaud
Published: December 2021

This paper reports on the manufacture of ZnO nanoparticles (ZnO NPs) from Prosopis juliflora leaf extracts. Various methods of characterization were used, including X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FT-IR), scanning electron microscope (SEM), and transmission electron microscope TEM. ZnO NPs has a hexagonal wurtzite structure with a preferred orientation of 101 planes, according to XRD. The functional groups found in ZnO NPs isolated from leaves are responsible for the FT-IR peaks that correspond to them. The morphology of the produced nanoparticles is a sphere-like form, as shown in the SEM pictures. TEM examination revealed ZnO NPs with a size of 50–55 nm. These ZnO NPs were used to remediate pollutants in paper mill effluents, and they were able to remove 86% of the organic pollutants from the sample at 0.05 mg/L dose and reduce 89% of the organic pollutants during a 5-h reflex time. Meanwhile, for the photocatalysis of paper mill effluents, it has been noted that COD was removed by 74.30%, 63.23%, and 57.96% for the first, second, and third cycles, respectively.
Analyzing Tuberculosis Reactivation in Patients with Rheumatoid Arthritis and Ankylosing Spondylitis Treated with Biological Therapy Using Machine Learning Methods
Authors: Mircea-Vicoveanu Andra-Maria , Rezuș Elena , Leon Florin , Curteanu Silvia
Published: December 2021

This study is based on the consideration that the patients with rheumatoid arthritis and ankylosing spondylitis undergoing biological therapy have a higher risk of developing tuberculosis. The QuantiFERON-TB Gold test result was the output of the models and a series of features related to the patients and their treatments were chosen as inputs. A distribution of patients by gender and biological therapy, followed at the time of inclusion in the study, and at the end of the study, is made for both rheumatoid arthritis and ankylosing spondylitis. A series of classification algorithms (random forest, nearest neighbor, k-nearest neighbors, C4.5 decision trees, non-nested generalized exemplars, and support vector machines) and attribute selection algorithms (ReliefF, InfoGain, and correlation-based feature selection) were successfully applied. Useful information was obtained regarding the influence of biological and classical treatments on tuberculosis risk, and most of them agreed with medical studies.
Evaluation of Unfilled Sheath in Concrete Structures Using Response Waveform in Time Domain
Authors: Ikebata Kota , Kobayashi Yoshikazu , Oda Kenichi , Nakamura Katsuya
Published: December 2021

The impact elastic wave method (IEW) has been applied to evaluate the thickness and internal defects of the target structure based on the dominant frequency of the response wave that is formed by the repeated reflections in the thickness direction. However, it is difficult to evaluate the size and position of the defect by IEW if the size and depth are relatively small and deep, respectively, and further, it is known that the technique is inapplicable if the target is not a plate-like structures. Therefore, the authors propose a new technique that uses Difference value as a new evaluation index to overcome the limitations of the conventional methods. Difference value shows the change of the response waveform in the time domain; it is computed by using a response waveform of the structures in sound condition as a reference. In this paper, the practicality of the Difference value is investigated by performing experiments using concrete specimens. The results of the experiments demonstrate that Difference value changes by the influence of internal defects, and Difference value evaluates the location of the relatively small defect that is difficult to evaluate by the conventional technique.
Lactic Acid Bacteria Co-Encapsulated with Lactobionic Acid: Probiotic Viability during In Vitro Digestion
Authors: Sáez-Orviz Sara , Passannanti Francesca , Gallo Marianna , Colucci Cante Rosa , Nigro Federica , Budelli Andrea Luigi , Rendueles Manuel , Nigro Roberto , Díaz Mario
Published: December 2021

Synbiotic products are a type of functional food with great potential due to consumer interest in foods that improve health and/or reduce the risk of certain diseases. In this study, synbiotic macrocapsules were developed using Lactobacillus paracasei CBA L74 as probiotic and lactobionic acid (LBA) as prebiotic. Firstly, the probiotic was proven to be able to use LBA as the only substrate source checking their growth and lactic acid production. Then, four different types of capsules were produced using sodium alginate as matrix and different hardener solutions (CaCl2 and chitosan). The macrocapsules were characterised regarding their strength, and the best performing ones were used for further analysis. In order to obtain a synbiotic capsule characterized by a longer stability time due to low water activity, the capsules were dried using freeze and thermal drying. Successively, to revitalize the microorganisms, the capsules were rehydrated in two different media (saline solution and yogurt), subjected to simulated in vitro digestion tests and visually characterised. In addition, their viability over time was assessed. As a result, L. paracasei was able to grow using LBA as the only source of carbon with better production of lactic acid for prolonged periods. The more resistant freeze- and thermal-dried capsules showed differences in rehydration kinetics, and visual changes were also observed. In simulated in vitro digestion tests, capsules rehydrated in yogurt showed the best results in terms of survival. Regarding their viability over time, the importance of the use of chitosan was noted.
A Temperature-Dependent Heat Source for Simulating Deep Penetration in Selective Laser Melting Process
Authors: Jia Yabo , Saadlaoui Yassine , Bergheau Jean-Michel
Published: December 2021

Numerical methods for simulating selective laser melting (SLM) have been widely carried out to understand the physical behaviors behind the process. Numerical simulation at the macroscale allows the relationship between input parameters (laser power, scanning speed, powder layer thickness, etc.) and output results (distortion, residual stress, etc.) to be investigated. However, the macroscale thermal models solved by the finite element method cannot predict the melt pool depth correctly as they ignore the effect of fluid flow in the melting pool, especially in the case of the presence of deep penetration. To remedy this limitation, an easy-implemented temperature-dependent heat source is proposed. This heat source can adjust its parameters during the simulation to compensate for these neglected thermal effects related to the fluid flow and keyhole, and the heat source’s parameters become fixed once the temperatures of the points of interest become stable. Contrary to the conventional heat source model, parameters of the proposed heat source do not require a calibration with experiments for each process parameter. The proposed model is validated by comparing its results with those of the anisotropic thermal conductivity method and experimental measurements.
Configuration of Non-Pumping Reactive Wells Considering Minimum Well Spacing
Authors: Goo Ja-Young , Kim Jae-Hyun , Lee Young Jae , Lee Soonjae
Published: December 2021

A non-pumping reactive well (NPRW) is a subsurface structure that prevents contaminant spread using many non-pumping wells containing reactive media. For the construction of an effective NPRW, a sufficiently small spacing between wells is an important design factor to prevent contaminant leakage. However, close well construction is not recommended because of concerns about the decreased stability of adjacent wells under field conditions. In this research, we proposed a sawtooth array of NPRW as a practical configuration to minimize well spacing while meeting stability requirements in the field. To evaluate the performance of the novel NPRW configurations, a numerical modeling was conducted considering different well diameters and well spacings and their performance was compared taking into account the number of wells and the mass of the reactive material. The comparison results showed that the sawtooth configuration was more practical than a line of wells. The performance curve of NPRWs with the saw-toothed configuration was constructed from the relationship between the contaminant removal and configuration components (diameter and spacing of the well). This can be used to predict the contaminant removal performance of NPRWs with a sawtooth array.
Silica-Based Composites with Enhanced Rheological Properties Thanks to a Nanosized Graphite Functionalized with Serinol Pyrrole
Authors: Prioglio Gea , Agnelli Silvia , Pandini Stefano , Galimberti Maurizio
Published: December 2021

Silica-based rubber composites have tremendous importance, as they allow the reduction in hysteresis in demanding dynamic-mechanical applications such as tire compounds and hence have a lower environmental impact. However, they also present drawbacks such as poor rheological behavior. In this work, an innovative silica-based hybrid filler system was developed, obtaining a rubber composite with an improved set of properties. A nanosized high surface area graphite (HSAG) was functionalized with 2-(2,5-dimethyl-1H-pyrrol-1-yl)propane-1,3-diol, serinol pyrrole (SP), through a simple process characterized by a high carbon efficiency. The HSAG-SP adduct, with about nine parts of SP per hundred parts of carbon filler, was used to form a hybrid filler system with silica. An elastomeric composite, with poly(styrene-co-butadiene) from anionic polymerization and poly(1,4-cis-isoprene) from Hevea brasiliensis was prepared with 50 parts of silica, which was replaced in a minor amount (15%) by either pristine HSAG or HSAG-SP. The best set of composite properties was obtained with HSAG-SP: the same dynamic rigidity and hysteresis and tensile properties of the silica-based material and appreciably better rheological properties, particularly in terms of flowability. This work paves the way for a new generation of silica-based composites, with improved properties, based on a hybrid filler system with a nanosized edge functionalized graphite.
Editorial for the Special Issue “Microbial Communities in Cultural Heritage and Their Control”
Authors: De Leo Filomena , Jurado Valme
Published: December 2021

This editorial focuses on the studies published within the present Special Issue presenting advances in the field of biodeterioration of cultural heritage caused by microbial communities with a particular focus on new methods for their elimination and control.
Time Delay in Electron Collision with a Spherical Target as a Function of the Scattering Angle
Authors: Amusia Miron Ya. , Baltenkov Arkadiy S. , Woiciechowski Igor
Published: December 2021

We have studied the angular time delay in slow-electron elastic scattering by spherical targets as well as the average time delay of electrons in this process. It is demonstrated how the angular time delay is connected to the Eisenbud–Wigner–Smith (EWS) time delay. The specific features of both angular and energy dependencies of these time delays are discussed in detail. The potentialities of the derived general formulas are illustrated by the numerical calculations of the time delays of slow electrons in the potential fields of both absolutely hard-sphere and delta-shell potential well of the same radius. The conducted studies shed more light on the specific features of these time delays.
Genetic, Epigenetic, Genomic and Microbial Approaches to Enhance Salt Tolerance of Plants: A Comprehensive Review
Authors: Saradadevi Gargi Prasad , Das Debajit , Mangrauthia Satendra K. , Mohapatra Sridev , Chikkaputtaiah Channakeshavaiah , Roorkiwal Manish , Solanki Manish , Sundaram Raman Meenakshi , Chirravuri Neeraja N. , Sakhare Akshay S. , Kota Suneetha , Varshney Rajeev K. , Mohannath Gireesha
Published: December 2021

Globally, soil salinity has been on the rise owing to various factors that are both human and environmental. The abiotic stress caused by soil salinity has become one of the most damaging abiotic stresses faced by crop plants, resulting in significant yield losses. Salt stress induces physiological and morphological modifications in plants as a result of significant changes in gene expression patterns and signal transduction cascades. In this comprehensive review, with a major focus on recent advances in the field of plant molecular biology, we discuss several approaches to enhance salinity tolerance in plants comprising various classical and advanced genetic and genetic engineering approaches, genomics and genome editing technologies, and plant growth-promoting rhizobacteria (PGPR)-based approaches. Furthermore, based on recent advances in the field of epigenetics, we propose novel approaches to create and exploit heritable genome-wide epigenetic variation in crop plants to enhance salinity tolerance. Specifically, we describe the concepts and the underlying principles of epigenetic recombinant inbred lines (epiRILs) and other epigenetic variants and methods to generate them. The proposed epigenetic approaches also have the potential to create additional genetic variation by modulating meiotic crossover frequency.
Tempol Alters Urinary Extracellular Vesicle Lipid Content and Release While Reducing Blood Pressure during the Development of Salt-Sensitive Hypertension
Authors: Chacko Kevin M. , Nouri Mohammad-Zaman , Schramm Whitney C. , Malik Zeeshan , Liu Lauren P. , Denslow Nancy D. , Alli Abdel A.
Published: December 2021

Salt-sensitive hypertension resulting from an increase in blood pressure after high dietary salt intake is associated with an increase in the production of reactive oxygen species (ROS). ROS are known to increase the activity of the epithelial sodium channel (ENaC), and therefore, they have an indirect effect on sodium retention and increasing blood pressure. Extracellular vesicles (EVs) carry various molecules including proteins, microRNAs, and lipids and play a role in intercellular communication and intracellular signaling in health and disease. We investigated changes in EV lipids, urinary electrolytes, osmolality, blood pressure, and expression of renal ENaC and its adaptor protein, MARCKS/MARCKS Like Protein 1 (MLP1) after administration of the antioxidant Tempol in salt-sensitive hypertensive 129Sv mice. Our results show Tempol infusion reduces systolic blood pressure and protein expression of the alpha subunit of ENaC and MARCKS in the kidney cortex of hypertensive 129Sv mice. Our lipidomic data show an enrichment of diacylglycerols and monoacylglycerols and reduction in ceramides, dihydroceramides, and triacylglycerols in urinary EVs from these mice after Tempol treatment. These data will provide insight into our understanding of mechanisms involving strategies aimed to inhibit ROS to alleviate salt-sensitive hypertension.
The Empowering Effect of Embodied Awareness Practice on Body Structural Map and Sensorimotor Activity: The Case of Feldenkrais Method
Authors: Crivelli Davide , Di Ruocco Massimilla , Balena Alessandra , Balconi Michela
Published: December 2021

While outcomes of embodied awareness practices in terms of improved posture and flexibility, movement efficiency, and well-being are often reported, systematic investigations of such training effects and of the actual nature, extent, and neurofunctional correlates of learning mechanisms thought to lie at the core of such practices are very limited. The present study focused on the Feldenkrais method (FM), one of the most established embodied awareness practices, and aimed at investigating the neurofunctional outcomes of the somatic learning process at the core of the method by testing the modulations induced by a standardized FM protocol on the complexity of practicers’ body structural map and on the activity of their sensorimotor network during different movement-related tasks (i.e., gestures observation, execution, and imagery). Twenty-five participants were randomly divided into an experimental group—which completed a 28-session FM protocol based on guided group practice—and a control group, and underwent pre-/post-training psychometric and electrophysiological assessment. Data analysis highlighted, at the end of the FM protocol, a significant increase of EEG markers of cortical activation (task-related mu desynchronization) in precentral regions during action observation and in central regions during action execution and imagery. Also, posterior regions of the sensorimotor network showed systematic activation during all the action-related tasks.
Cognitive and Neural Mechanisms of Social Communication Dysfunction in Primary Progressive Aphasia
Authors: Goldberg Zoë-Lee , El-Omar Hashim , Foxe David , Leyton Cristian E. , Ahmed Rebekah M. , Piguet Olivier , Irish Muireann
Published: December 2021

Mounting evidence suggests that, in parallel with well-defined changes in language, primary progressive aphasia (PPA) syndromes display co-occurring social cognitive impairments. Here, we explored multidimensional profiles of carer-rated social communication using the La Trobe Communication Questionnaire (LCQ) in 11 semantic dementia (SD), 12 logopenic progressive aphasia (LPA) and 9 progressive non-fluent aphasia (PNFA) cases and contrasted their performance with 19 Alzheimer’s disease (AD) cases, 26 behavioural variant frontotemporal dementia (bvFTD) cases and 31 healthy older controls. Relative to the controls, the majority of patient groups displayed significant overall social communication difficulties, with common and unique profiles of impairment evident on the LCQ subscales. Correlation analyses revealed a differential impact of social communication disturbances on functional outcomes in patient and carer well-being, most pronounced for SD and bvFTD. Finally, voxel-based morphometry analyses based on a structural brain MRI pointed to the degradation of a distributed brain network in mediating social communication dysfunction in dementia. Our findings suggest that social communication difficulties are an important feature of PPA, with significant implications for patient function and carer well-being. The origins of these changes are likely to be multifactorial, reflecting the breakdown of fronto-thalamic brain circuits specialised in the integration of complex information.
Higher Level of Sports Activities Participation during Five-Year Survival Is Associated with Better Quality of Life among Chinese Breast Cancer Survivors
Authors: Lei Yuan-Yuan , Ho Suzanne C. , Cheung Ka-Li , Yeo Victoria A. , Lee Roselle , Kwok Carol , Cheng Ashley , Mo Frankie K. F. , Yeo Winnie
Published: December 2021

Evidence about the association between the level of sports activities participation post-diagnosis and quality of life (QoL) among Chinese women with early-stage breast cancer is limited. A validated modified Chinese Baecke questionnaire was used to prospectively measure sports activities among a breast cancer cohort at four time-points: baseline and 18, 36, and 60 months after diagnosis (sports activities during the previous 12 months before each interview); QoL was measured at the same time. In total, 1289, 1125, and 1116 patients were included in the analyses at 18-, 36- and 60-month follow-up, respectively. The percentages of patients who belonged to no (0 metabolic equivalent of task (MET)-hours per week), low-level (<10 MET-hours/week), and high-level (≥10 MET-hours/week) sports activities group were 20.7%, 45.5%, and 33.8% at 18-month follow-up, respectively; the corresponding figures at 36 and 60 months differed slightly. Using data from the three follow-ups, generalized estimating equation (GEE) analyses showed that higher levels of sports activities participation were associated with better QoL in several items as well as fewer symptoms. The present findings in Chinese women with breast cancer provide important evidence on the beneficial effect of regular participation in sports activities following cancer diagnosis on patients’ QoL.
Immunotherapy of Ovarian Cancer with Particular Emphasis on the PD-1/PDL-1 as Target Points
Authors: Świderska Janina , Kozłowski Mateusz , Kwiatkowski Sebastian , Cymbaluk-Płoska Aneta
Published: December 2021

Ovarian cancer is one of the most fatal cancers in women worldwide. Cytoreductive surgery combined with platinum-based chemotherapy has been the current first-line treatment standard. Nevertheless, ovarian cancer appears to have a high recurrence rate and mortality. Immunological processes play a significant role in tumorigenesis. The production of ligands for checkpoint receptors can be a very effective, and undesirable, immunosuppressive mechanism for cancers. The CTLA-4 protein, as well as the PD-1 receptor and its PD-L1 ligand, are among the better-known components of the control points. The aim of this paper was to review current research on immunotherapy in the treatment of ovarian cancer. The authors specifically considered immune checkpoints molecules such as PD-1/PDL-1 as targets for immunotherapy. We found that immune checkpoint-inhibitor therapy does not have an improved prognosis in ovarian cancer; although early trials showed that a combination of anti-PD-1/PD-L1 therapy with targeted therapy might have the potential to improve responses and outcomes in selected patients. However, we must wait for the final results of the trials. It seems important to identify a group of patients who could benefit significantly from treatment with immune checkpoints inhibitors. However, despite numerous trials, ICIs have not become part of routine clinical practice for the treatment of ovarian cancer.
Identification of Survival and Therapeutic Response-Related Ferroptosis Regulators in Bladder Cancer through Data Mining and Experimental Validation
Authors: Zhang Pu , Liu Zijian , Wang Decai , Li Yunxue , Zhang Yuan , Xiao Yajun
Published: December 2021

Ferroptosis has been reported to regulate tumorigenesis, metastasis, drug resistance and the immune response. However, the potential roles of ferroptosis regulators in the advancement of bladder cancer remain to be explored. We systematically evaluated the multidimensional alteration landscape of ferroptosis regulators in bladder cancer and checked if their expression correlated with the ferroptosis index. We used least absolute shrinkage and selection operator regression to form a signature consisting of seven ferroptosis regulator. We confirmed the signature’s prognostic and predictive accuracy with five independent datasets. A nomogram was built to predict the overall survival and risk of death of patients. The relative expression of the genes involved in the signature was also clarified by real-time quantitative PCR. We found the risk score was related to tumor progression and antitumor immunity-related pathways. Moreover, there existed negative association between the relative antitumor immune cell infiltration level and the risk score, and higher tumor mutation burden was found in the group of lower risk score. We used The Tumor Immune Dysfunction and Exclusion database and IMvigor210 cohort having immunotherapy efficacy results to confirm the prediction function of the risk score. Furthermore, the ferroptosis regulator signature could also reflect the chemotherapy sensitivity of bladder cancer.
Oncologic Impact and Safety of Pre-Operative Radiotherapy in Localized Prostate and Bladder Cancer: A Comprehensive Review from the Cancerology Committee of the Association Française d’Urologie
Authors: Sargos Paul , Supiot Stéphane , Créhange Gilles , Fromont-Hankard Gaëlle , Barret Eric , Beauval Jean-Baptiste , Brureau Laurent , Dariane Charles , Fiard Gaëlle , Gauthé Mathieu , Mathieu Romain , Roubaud Guilhem , Ruffion Alain , Renard-Penna Raphaële , Neuzillet Yann , Rouprêt Morgan , Ploussard Guillaume
Published: December 2021

Preoperative radiotherapy (RT) is commonly used for the treatment of various malignancies, including sarcomas, rectal, and gynaecological cancers, but it is preferentially used as a competitive treatment to radical surgery in uro-oncology or as a salvage procedure in cases of local recurrence. Nevertheless, preoperative RT represents an attractive strategy to prevent from intraoperative tumor seeding in the operative field, to sterilize microscopic extension outside the organ, and to enhance the pathological and/or imaging tumor response rate. Several clinical works support this research field in uro-oncology. In this review article, we summarized the oncologic impact and safety of preoperative RT in localized prostate and muscle-invasive bladder cancer. Preliminary studies suggest that both modalities can be complementary as initial primary tumor treatments and that a pre-operative radiotherapy strategy could be beneficial in a well-defined population of patients who are at a very high-risk of local relapse. Future prospective trials are warranted to evaluate the oncologic benefit of such a combination of local treatments in addition to new life-prolonging systemic therapies, such as immunotherapy, and new generation hormone therapies. Moreover, the safety and the feasibility of salvage surgical procedures due to non-response or local recurrence after pelvic RT remain poorly evaluated in that context.
Structural, Optical, and Catalytic Properties of MgCr2O4 Spinel-Type Nanostructures Synthesized by Sol–Gel Auto-Combustion Method
Authors: Mykhailovych Vasyl , Kanak Andrii , Cojocaru Ştefana , Chitoiu-Arsene Elena-Daniela , Palamaru Mircea Nicolae , Iordan Alexandra-Raluca , Korovyanko Oleksandra , Diaconu Andrei , Ciobanu Viorela Gabriela , Caruntu Gabriel , Lushchak Oleh , Fochuk Petro , Khalavka Yuriy , Rotaru Aurelian
Published: December 2021

Spinel chromite nanoparticles are prospective candidates for a variety of applications from catalysis to depollution. In this work, we used a sol–gel auto-combustion method to synthesize spinel-type MgCr2O4 nanoparticles by using fructose (FS), tartaric acid (TA), and hexamethylenetetramine (HMTA) as chelating/fuel agents. The optimal temperature treatment for the formation of impurity-free MgCr2O4 nanostructures was found to range from 500 to 750 °C. Fourier transform infrared (FTIR) spectroscopy was used to determine the lattice vibrations of the corresponding chemical bonds from octahedral and tetrahedral positions, and the optical band gap was calculated from UV–VIS spectrophotometry. The stabilization of the spinel phase was proved by X-ray diffraction (XRD) and energy-dispersive X-ray (EDX) analysis. From field-emission scanning electron microscopy (FE-SEM), we found that the size of the constituent particles ranged from 10 to 40 nm. The catalytic activity of the as-prepared MgCr2O4 nanocrystals synthesized by using tartaric acid as a chelating/fuel agent was tested on the decomposition of hydrogen peroxide. In particular, we found that the nature of the chelating/fuel agent as well as the energy released during the auto-combustion played an important role on the structural, optical, and catalytic properties of MgCr2O4 nanoparticles obtained by this synthetic route.
Phenobarbital Induces SLC13A5 Expression through Activation of PXR but Not CAR in Human Primary Hepatocytes
Authors: Li Zhihui , Li Linhao , Heyward Scott , Men Shuaiqian , Xu Meishu , Sueyoshi Tatsuya , Wang Hongbing
Published: December 2021

Phenobarbital (PB), a widely used antiepileptic drug, is known to upregulate the expression of numerous drug-metabolizing enzymes and transporters in the liver primarily via activation of the constitutive androstane receptor (CAR, NR1I3). The solute carrier family 13 member 5 (SLC13A5), a sodium-coupled citrate transporter, plays an important role in intracellular citrate homeostasis that is associated with a number of metabolic syndromes and neurological disorders. Here, we show that PB markedly elevates the expression of SLC13A5 through a pregnane X receptor (PXR)-dependent but CAR-independent signaling pathway. In human primary hepatocytes, the mRNA and protein expression of SLC13A5 was robustly induced by PB treatment, while genetic knockdown or pharmacological inhibition of PXR significantly attenuated this induction. Utilizing genetically modified HepaRG cells, we found that PB induces SLC13A5 expression in both wild type and CAR-knockout HepaRG cells, whereas such induction was fully abolished in the PXR-knockout HepaRG cells. Mechanistically, we identified and functionally characterized three enhancer modules located upstream from the transcription start site or introns of the SLC13A5 gene that are associated with the regulation of PXR-mediated SLC13A5 induction. Moreover, metformin, a deactivator of PXR, dramatically suppressed PB-mediated induction of hepatic SLC13A5 as well as its activation of the SLC13A5 luciferase reporter activity via PXR. Collectively, these data reveal PB as a potent inducer of SLC13A5 through the activation of PXR but not CAR in human primary hepatocytes.
Platelet Activation Mechanisms and Consequences of Immune Thrombocytopenia
Authors: Sun Siyu , Urbanus Rolf T. , ten Cate Hugo , de Groot Philip G. , de Laat Bas , Heemskerk Johan W. M. , Roest Mark
Published: December 2021

Autoimmune disorders are often associated with low platelet count or thrombocytopenia. In immune-induced thrombocytopenia (IIT), a common mechanism is increased platelet activity, which can have an increased risk of thrombosis. In addition, or alternatively, auto-antibodies suppress platelet formation or augment platelet clearance. Effects of the auto-antibodies are linked to the unique structural and functional characteristics of platelets. Conversely, prior platelet activation may contribute to the innate and adaptive immune responses. Extensive interplay between platelets, coagulation and complement activation processes may aggravate the pathology. Here, we present an overview of the reported molecular causes and consequences of IIT in the most common forms of autoimmune disorders. These include idiopathic thrombocytopenic purpura (ITP), systemic lupus erythematosus (SLE), antiphospholipid syndrome (APS), drug-induced thrombocytopenia (DITP), heparin-induced thrombocytopenia (HIT), COVID-19 vaccine-induced thrombosis with thrombocytopenia (VITT), thrombotic thrombocytopenia purpura (TTP), and hemolysis, the elevated liver enzymes and low platelet (HELLP) syndrome. We focus on the platelet receptors that bind auto-antibodies, the immune complexes, damage-associated molecular patterns (DAMPs) and complement factors. In addition, we review how circulating platelets serve as a reservoir of immunomodulatory molecules. By this update on the molecular mechanisms and the roles of platelets in the pathogenesis of autoimmune diseases, we highlight platelet-based pathways that can predispose for thrombocytopenia and are linked thrombotic or bleeding events.
Acute Infectious Gastroenteritis: The Causative Agents, Omics-Based Detection of Antigens and Novel Biomarkers
Authors: Hasan Haziqah , Nasirudeen Nor Ashika , Ruzlan Muhammad Alif Farhan , Mohd Jamil Muhammad Aiman , Ismail Noor Akmal Shareela , Wahab Asrul Abdul , Ali Adli
Published: December 2021

Acute infectious gastroenteritis (AGE) is among the leading causes of mortality in children less than 5 years of age worldwide. There are many causative agents that lead to this infection, with rotavirus being the commonest pathogen in the past decade. However, this trend is now being progressively replaced by another agent, which is the norovirus. Apart from the viruses, bacteria such as Salmonella and Escherichia coli and parasites such as Entamoeba histolytica also contribute to AGE. These agents can be recognised by their respective biological markers, which are mainly the specific antigens or genes to determine the causative pathogen. In conjunction to that, omics technologies are currently providing crucial insights into the diagnosis of acute infectious gastroenteritis at the molecular level. Recent advancement in omics technologies could be an important tool to further elucidate the potential causative agents for AGE. This review will explore the current available biomarkers and antigens available for the diagnosis and management of the different causative agents of AGE. Despite the high-priced multi-omics approaches, the idea for utilization of these technologies is to allow more robust discovery of novel antigens and biomarkers related to management AGE, which eventually can be developed using easier and cheaper detection methods for future clinical setting. Thus, prediction of prognosis, virulence and drug susceptibility for active infections can be obtained. Case management, risk prediction for hospital-acquired infections, outbreak detection, and antimicrobial accountability are aimed for further improvement by integrating these capabilities into a new clinical workflow.
Sea State Decadal Variability in the North Atlantic: A Review
Authors: Hochet Antoine , Dodet Guillaume , Ardhuin Fabrice , Hemer Mark , Young Ian
Published: December 2021

Long-term changes of wind-generated ocean waves have important consequences for marine engineering, coastal management, ship routing, and marine spatial planning. It is well-known that the multi-annual variability of wave parameters in the North Atlantic is tightly linked to natural fluctuations of the atmospheric circulation, such as the North Atlantic Oscillation. However, anthropogenic climate change is also expected to influence sea states over the long-term through the modification of atmospheric and ocean circulation and melting of sea ice. Due to the relatively short duration of historical sea state observations and the significant multi-decadal variability in the sea state signal, disentangling the anthropogenic signal from the natural variability is a challenging task. In this article, the literature on inter-annual to multi-decadal variability of sea states in the North Atlantic is reviewed using data from both observations and model reanalysis.
On the Use of Ensemble Predictions for Parametric Typhoon Insurance
Authors: Ng Kelvin S. , Leckebusch Gregor C. , Ye Qian , Ying Wenwen , Zhao Haoran
Published: December 2021

Parametric typhoon insurances are an increasingly used financial tool to mitigate the enormous impact of tropical cyclones, as they can quickly distribute much-needed resources, e.g., for post-disaster recovery. In order to optimise the reliability and efficiency of parametric insurance, it is essential to have well-defined trigger points for any post-disaster payout. This requires a robust localised hazard assessment for a given region. However, due to the rarity of severe, landfalling tropical cyclones, it is difficult to obtain a robust hazard assessment based on historical observations. A recent approach makes use of unrealised, high impact tropical cyclones from state-of-the-art ensemble prediction systems to build a physically consistent event set, which would be equivalent to about 10,000 years of observations. In this study, we demonstrate that (1) alternative trigger points of parametric typhoon insurance can be constructed from a local perspective and the added value of such trigger points can be analysed by comparing with an experimental set-up informed by current practice; (2) the estimation of the occurrence of tropical cyclone-related losses on the provincial level can be improved. We further discuss the potential future development of a general tropical cyclone compound parametric insurance.
Effect of Low Magnetic Field on Biomimetic Deposition of Hydroxyapatite on Titanium and BIOLINE Stainless Steel
Authors: Uribe Rafael , Uvillús Andrea , Bonilla Omar , Lascano Luis , González Gema
Published: December 2021

In this work, we evaluated the effect of a low magnetic field on the deposition of hydroxyapatite (HAp) on different metallic substrates. The substrates studied were titanium and BIOLINE stainless steel SS316LVM with and without Ta and TaN/Ta coatings. Before deposition, the uncoated Ti and SS316LVM substrates were treated with alkali to improve the adhesion of the films prompted to be formed. Next, all substrates (coated and uncoated) were immersed in stimulated body fluid (SBF) at physiological conditions of 37 °C, pH = 7.4, in the presence of magnetic fields from 0.15 T and 0.22 T for 7, 10, and 14 days. The formed films were characterized using SEM, FTIR, and the contact angle. Ti and SS316LVM substrates presented Ca/P relations closer to the stoichiometric HAp. It was demonstrated that in both coatings, Ta and Ta/N, an increase of the bioactivity was obtained. Additionally, our results showed that the application of magnetic fields has a significant effect on the increment in the mass:area ratio of HAp. Finally, the contact angle values were lower than 90°, showing an increase in hydrophilicity with respect to the metallic substrates.
Co-Deposition Mechanisms of Calcium Sulfate and Calcium Carbonate Scale in Produced Water
Authors: Yan Yan , Yu Tao , Zhang Huan , Song Jiayu , Qu Chengtun , Li Jinling , Yang Bo
Published: December 2021

Co-precipitation of mineral-based salts during scaling remains poorly understood and thermodynamically undefined within the water industry. This study focuses on investigating calcium carbonate and calcium sulfate mixed precipitation in scaling. Scaling is often observed in the produced water supply as a result of treatment processes. Co-precipitation results were compared with experimental results of a single salt crystallization. Several parameters were carefully monitored, including the electrical conductivity, pH value, crystal morphology and crystal form. The existence of the calcium carbonate scale in the mixed system encourages the loose calcium sulfate scale to become more tightly packed. The mixed scale was firmly adhered to the beaker, and the adhesion of the co-deposition product was located between the pure calcium sulfate scale and the pure calcium carbonate scale. The crystalline form of calcium sulfate was gypsum in both pure material deposition and mixed deposition, while the calcium carbonate scale was stable in calcite form in the pure material deposition. In the co-deposition, apart from calcite form, some calcium carbonate scale crystals had metastable vaterite form. This indicated that the presence of SO42− ions reduced the energy barrier of the calcium carbonate scale and hindered its transformation from a vaterite form to a calcite one, and the increase in HCO3− content inhibited the formation of calcium sulfate scale.
A Synopsis of Signaling Crosstalk of Pericytes and Endothelial Cells in Salivary Gland
Authors: Cucu Ioana , Nicolescu Mihnea
Published: December 2021

The salivary gland (SG) microvasculature constitutes a dynamic cellular organization instrumental to preserving tissue stability and homeostasis. The interplay between pericytes (PCs) and endothelial cells (ECs) culminates as a key ingredient that coordinates the development, maturation, and integrity of vessel building blocks. PCs, as a variety of mesenchymal stem cells, enthrall in the field of regenerative medicine, supporting the notion of regeneration and repair. PC-EC interconnections are pivotal in the kinetic and intricate process of angiogenesis during both embryological and post-natal development. The disruption of this complex interlinkage corresponds to SG pathogenesis, including inflammation, autoimmune disorders (Sjögren’s syndrome), and tumorigenesis. Here, we provided a global portrayal of major signaling pathways between PCs and ECs that cooperate to enhance vascular steadiness through the synergistic interchange. Additionally, we delineated how the crosstalk among molecular networks affiliate to contribute to a malignant context. Additionally, within SG microarchitecture, telocytes and myoepithelial cells assemble a labyrinthine companionship, which together with PCs appear to synchronize the regenerative potential of parenchymal constituents. By underscoring the intricacy of signaling cascades within cellular latticework, this review sketched a perceptive basis for target-selective drugs to safeguard SG function.