bluuebunny/crossref_metadata_2025 huggingface dataset:
The Role of Antibiotics in Endoscopic Transmural Drainage of Post-Inflammatory Pancreatic and Peripancreatic Fluid Collections
Authors: Jagielski Mateusz , Kupczyk Wojciech , Piątkowski Jacek , Jackowski Marek
Published: July 2022

Background

Although endoscopic treatment of symptomatic post-inflammatory pancreatic and peripancreatic fluid collections (PPPFCs) is an established treatment method, some aspects of endotherapy and periprocedural management remain controversial. The role of antibiotics is one of the most controversial issues in interventional endoscopic management of local complications of pancreatitis.

Methods

This study was a randomized, non-inferiority, placebo-controlled, and double-blinded clinical trial to investigate the role of antibiotic prophylaxis in endoscopic transmural drainage in patients with symptomatic non-infected PPPFCs and assess the influence of antibiotic treatment on the results of endotherapy in patients with symptomatic infected PPPFCs.

This trial included 62 patients treated endoscopically for PPPFCs in 2020 at our medical center. Patients were divided into two groups; group 1 comprised patients who had received empirical intravenous antibiotic therapy during endotherapy and group 2 comprised patients who did not receive antibiotic therapy during endoscopic drainage of PPPFCs. The end points were clinical success and long-term success of endoscopic treatment.

Results

Thirty-one patients were included in group 1 (walled-off pancreatic necrosis [WOPN, 51.6%; pseudocyst, 48.4%) and 31 patients in group 2 (WOPN, 58.1%; pseudocyst, 41.9%) (p=0.6098/nonsignificant statistical [NS]). Infection with PPPFCs was observed in 15/31 (48.39%) patients in group 1 and in 15/31 (48.39%) patients in group 2 (p=1.0/NS). The average time of active (with flushing through nasocystic drainage) drainage in group 1 was 13.0 (6 – 21) days and was 14.0 (7 – 25) days in group 2 (p=0.405/NS). The average total number endoscopic procedures on one patient was 3.3 (2 – 5) in group 1 and 3.4 (2 – 7) in group 2 (p=0.899/NS). Clinical success of PPPFCs was observed in 29/31 (93.5%) patients from group 1 and in 30/31 (96.8%) patients from group 2 (p=0.5540/NS). Complications of endotherapy were noted in 8/31 (25.8%) patients in group 1 and in 10/31 (32.3%) patients in group 2 (p=0.576/NS). Long-term success in group 1 and 2 was reported in 26/31 (83.9%) and 24/31 (77.4%) patients, respectively (p=0.520/NS).

Conclusions

The effective endoscopic drainage of sterile PPPFCs requires no preventive or prophylactic use of antibiotics. In infected PPPFCs, antibiotic therapy is not required for effective endoscopic transmural drainage.
Iconic Associations Between Vowel Acoustics and Musical Patterns, and the Musical Protolanguage Hypothesis
Authors: Fenk-Oczlon Gertraud
Published: July 2022

Vowels are the most musical and sonic elements of speech. Previous studies found non-arbitrary associations between vowel intrinsic pitch and musical pitch in senseless syllables. In songs containing strings of senseless syllables, vowels are connected to melodic direction in close correspondence to theirintrinsic pitchor the frequency of the second formant F2. This paper shows that alsovowel intrinsic durationis related to musical patterns. It is generally assumed that low vowels like [a ɔ o] have a higher intrinsic duration than high vowels like [i y u] and that there is a positive correlation between the first formant F1 and duration. Analyzing 20 traditional Alpine yodels I found that vowels with longer intrinsic duration tend to align with longer notes, whereas vowels with shorter intrinsic duration with shorter notes. This new result might shed some light on size-sound symbolism in general: Since there is a direct match between vowel intrinsic duration and the “size” of musical notes, there is no need to explain the “size” of musical notes via Ohala's “frequency code” hypothesis. Moreover, I will argue that the iconic associations found between vowel acoustics and musical patterns support the idea of a sound-symbolic musical protolanguage. Such a protolanguage may have started with vowel syllables conveying pitch, timbre, as well as emotional, indexical, and sound-symbolic information.
Cardioprotective Effect of Paeonol on Chronic Heart Failure Induced by Doxorubicin via Regulating the miR-21-5p/S-Phase Kinase-Associated Protein 2 Axis
Authors: Chen Cong , Liu Shuhong , Cao Gaozhen , Hu Yang , Wang Run , Wu Min , Liu Mingya , Yiu Kai Hang
Published: July 2022

Background

This study primarily explored the role of paeonol in doxorubicin (DOX)-induced chronic heart failure (CHF), considering the cardioprotective effect of paeonol on an epirubicin-induced cardiac injury.

Methods

DOX-induced CHF-modeled rats were treated with paeonol. Cardiac function and myocardial damage in rats were evaluated by using the multifunction instrument, and the histopathology, apoptosis, and the expression of miR-21-5p and S-phase kinase-associated protein 2 (SKP2) in myocardium were detected. The target gene of miR-21-5p was confirmed by a dual-luciferase reporter assay. After the required transfection or paeonol treatment, the viability, apoptosis, mitochondrial membrane potential (MMP), and reactive oxygen species (ROS) of the DOX-induced cardiomyocytes were determined. Reverse-transcription quantitative-PCR (RT-qPCR) and Western blot were performed to quantify the expressions of miR-21-5p, SKP2, and apoptosis-related factors.

Results

Paeonol improved cardiac function and also ameliorated the cardiac damage of CHF-modeled rats, where the downregulation of abnormally elevated myocardial damage markers, including brain natriuretic peptide, lactate dehydrogenase, renin, angiotensin II, aldosterone, and endothelin 1, was observed. Paeonol alleviated the histopathological injury and suppressed the apoptosis in CHF-modeled rats, inhibited miR-21-5p expression, and upregulated SKP2 expression in vitro and in vivo. miR-21-5p targeted SKP2. Paeonol and SKP2 increased the viability and MMP, but reduced apoptosis and ROS in the DOX-induced cardiomyocytes. miR-21-5p exerted effects opposite to PAE and SKP2, and it downregulated the expression of Bcl-2 and mitochondrion-Cytochrome c (Cyt c) and upregulated the expression of Bax, C-caspase-3, and cytoplasm-Cyt c. miR-21-5p reversed the effects of paeonol, and its effects were further reversed by SKP2.

Conclusion

Paeonol shows a cardioprotective effect on DOX-induced CHF via regulating the miR-21-5p/SKP2 axis.
Pulmonary Blood Volume Measured by Dual-Energy Computed Tomography Can Help Distinguish Patients With Pulmonary Hypertension
Authors: Rezaei-Kalantari Kiara , Samimi Kaveh , Zomorodian Hamid , Bakhshandeh Hooman , Jafari Maryam , Farahmand Ali Mohammad , Pourseyedian Taleb , Sharifian Maedeh , Qanadli Salah Dine
Published: July 2022

Purpose

To evaluate the correlation between whole lung enhancement (WLE) and pulmonary blood volume (PBV) obtained through dual energy computed tomography pulmonary angiography (DECTPA) and echocardiography-derived systolic pulmonary arterial pressure (SPAP).

Methods

Sixty-eight patients who underwent DECTPA were enrolled in the study after giving informed consent. A transthoracic echocardiography was performed for all the subjects within 48 h of their DECTPA study to measure SPAP. The correlation of the two DECTPA-derived parameters, WLE and PBV, with SPAP was assessed. In addition, the predictive strength of these parameters was compared with that of traditional computed tomography (CT) signs of pulmonary hypertension (PH).

Results

The SPAP value showed a moderate correlation with main pulmonary artery (MPA) diameter (r = 0.48, P < 0.001), while having a weak correlation with WLE (r = −0.33, P = 0.007), PBV (r = −0.31, P = 0.01) and MPA/ascending aorta (MPA/AA) ratio (r = 0.26, P = 0.03). On regression analysis, MPA diameter (B ± SE: 1.8 ± 0.6, P = 0.004) and WLE (B ± SE: −0.5 ± 0.3, P = 0.042) had significant association with SPAP. In addition, SPAP ≥30 mmHg was related to the right to left ventricular diameter (RV/LV) ratio [OR (CI 95%): 24.39 (1.3–573.2), P = 0.04] and reversely associated with PBV [OR (CI 95%): 0.96 (0.93–0.98), P = 0.005]. Acquired cutoff value of 83% for PBV showed sensitivity and specificity of 73% to identify SPAP ≥30 mmHg [AUC (CI 95%):0.727 (0.588–0.866), P = 0.008].

Conclusions

Automated postprocessing calculation of iodine distribution analysis by DECTPA could be considered as an adjunctive tool to investigate for PH.
Investigation of the Global Seismic Noise Properties in Connection to Strong Earthquakes
Authors: Lyubushin Alexey
Published: July 2022

The global seismic noise, recorded on a network of 229 broadband seismic stations located around the globe for 25 years, from the beginning of 1997 to the end of 2021, has been investigated. To study the noise properties a set of statistics estimated daily have been used: the multifractal singularity spectrum support width, the minimum entropy of the squared wavelet coefficients, and the wavelet-based Donoho-Johnstone (DJ) index. It is shown that the time points of local extrema of the average values of the analyzed noise properties (minima for singularity spectrum support width and DJ-index and maxima for entropy) tend to occur before strong earthquakes. The time interval from the middle of 2002 to the middle of 2003 is determined, when the trend of decreasing the average coherence of the noise properties in the auxiliary network of 50 reference points changed to an increase. Along with an increase in the average coherence, there is an increase in the radius of the spatial maximum coherences of noise properties. Both of these trends continue until the end of 2021, which is interpreted as a general sign of an increase in the degree of criticality of the state of the planet and, as a result, an increase in global seismic danger. After two mega-earthquakes close in time: 27 February 2010, M=8.8 in Chile and 11 March 2011, M=9.1 in Japan, there was an increase in the spatial scales of the strong coherence of noise parameters, which is a sign of an increase in the critical state. The response of seismic noise properties to variations in the length of the day (LOD) has been studied. An estimate of the correlation function between the mean values of the response to LOD and the logarithm of the released seismic energy in a time window of 1 year indicates a delay in energy release with respect to the maxima of the response of noise properties to LOD with a delay time of about 500 days. In connection with this result, an additional intrigue is the extreme increase in the average value of the response to LOD in 2021.
Interdecadal Variability of Spring Eurasian Snowmelt and Its Impact on Eastern China Summer Precipitation
Authors: Cheng Fei , Li Qiaoping , Wang Jing , Liu Yanju , Ding Yihui , Shen Xinyong , Song Chengyu
Published: July 2022

In this study, the interdecadal variability of Eurasian spring snowmelt and its relationship with Eastern China summer precipitation (ECSP) are investigated based on observations and reanalysis data. Results show that the second mode of the empirical orthogonal function (EOF) analysis of the spring snowmelt, featured as a west-east dipole pattern, displays two interdecadal changes near the late-1970s and in the mid-2000s. The increased spring snowmelt over the Western Siberia (WSI) and the opposite situation over the Eastern Europe (EEU) are significantly linked to a meridional quadrupole summertime rainfall pattern on interdecadal time scales, with excessive rainfall over the regions of southern China (SC) and the Huang-Huai River (HHR) and deficient rainfall over the middle and lower reaches of Yangtze River Valley (YRV) and Inner Mongolia-northeastern China (IMNC). Besides, the possible mechanisms are discussed from the perspective of the hydrological effect related to snowmelt-induced soil moisture. Increased spring snowmelt can produce more water inflowing into the soil, leading to anomalous soil moisture, which can persist into summer. Excessive (deficient) snowmelt-related soil moisture anomalies over the WSI (EEU) in summer will change local land thermal conditions and thus generate cyclonic (anticyclonic) circulations with a quasi-barotropic structure. Therefore, the eastward-propagating wave-activity flux (WAF) could be strengthened over the WSI and EEU in the mid- and upper-troposphere and further propagate downstream. Combining the local response of atmospheric circulations to summer surface heating induced by local snowmelt-related SM anomalies over Mongolia, an anomalous anticyclonic (cyclonic) circulation near the Lake Baikal (SC) and the related strong descending (ascending) motion over the IMNC (SC) are founded. Besides, strong positive (negative) upper-level divergence appears over the YRV (HHR), which is the right (left) side of the exit region of upper-level jet. The upper-level divergence could be attributed to the convergence (divergence) of meridional winds on the right side of the anticyclonic circulation, thus the uniform descending (ascending) motion also appears over the YRV (HHR) through the collocation of upper-level convergence (divergence) and the compensatory lower-level circulations. Consequently, a quadrupole pattern of secondary circulation over Eastern China is founded, thereby causing the anomalously distributed ECSP under favorable water vapor conditions.
Students’ Perception and Performance Regarding Structured Query Language Through Online and Face-to-Face Learning
Authors: Elalouf Amir , Edelman Arik , Sever Dafna , Cohen Shaked , Ovadia Reut , Agami Omer , Shayhet Yulia
Published: July 2022

This study explores the Structured Query Language (SQL) learners’ perceptions in online and face-to-face learning regarding the role of the instructor, clarity in lesson delivery and understanding, and concerns about the shift in learning mode. In parallel, we evaluate the performance of online and face-to-face SQL learners in the final examination. The COVID-19 pandemic has forced educational institutes to shift their activities online. Thus, online learning has been accepted during the pandemic and gradually evolving. The literature on online and face-to-face learning has evaluated limited variables. Yet, in online and face-to-face learning, critical parameters concerning the SQL learners’ perceptions about the role of instructors have not been explored. The present study surveyed the final-year students learning medium-level SQL courses at Bar-Ilan University Israel and the College of Management Academic Studies Israel. Survey questionnaires included demographic information, online learning experience, online learning sources, and ten questions about the learners’ concerns of shifting, effectiveness, adequate instructions, the lecturer’s clarity during instruction, clear understanding of the lesson, instructor’s tools, instructor’s availability, satisfactory response, learning independence, and spending extra time in online and face-to-face learning, separately. This study included 102 online learners and 95 face-to-face learners. All the online learners used Zoom and WhatsApp, and the face-to-face learners used Gmail and WhatsApp. Both online and face-to-face learners were significantly satisfied with the lecturer’s performance, especially with the clarity in lecture delivery, instructor availability, and satisfactory response from the lecturer. In addition, online learners agreed upon the effective way of learning, clear understanding of the lesson, independence, and spending extra time. In contrast, face-to-face learners were more satisfied with the tools of the lecturer and dissatisfied with the dependence on the lecturer. Female students attending face-to-face learning were more concerned about the shift in the mode of learning. Further, online learners performed better in written examinations and face-to-face learners in oral examinations. Notwithstanding, advancements are still required to redesign the online learning environment for critical thinking in higher education.
Substantial Increase in Heavy Precipitation Events Preceded by Moist Heatwaves Over China During 1961–2019
Authors: Li Chenxi , Min Ruiying , Gu Xihui , Gulakhmadov Aminjon , Luo Sijia , Liu Ruihan , Slater Louise J. , Xie Fenghua , Kong Dongdong , Liu Jianyu , Li Yanan
Published: July 2022

Both moist heatwaves (HWs) and heavy precipitation events (HP) have increased in both frequency and magnitude over China in recent decades. However, the relationship between HW and HP and changes in the lagged coincidence of events (i.e., the occurrence of an HP event several days after an HW event, noted HWHP) remain unknown. We show here that HWHP events account for nearly one-third of HP events over China in summer, with high values in North China, Northeast China, and the East arid zone. HWHP events assessed using the heat index and the wet-bulb temperature methods increased by 45.25 and 23.97% from 1961 to 2019, respectively. These concurrent HWHP events tend to be spatially clustered, and the areas affected simultaneously have grown significantly. The increase in HW is the major driver of these changes in HWHP events, except in the western arid zone and North China. Our findings provide an understanding of the spatiotemporal changes in HWHP events over China and their implications for disaster mitigation.
Biobehavioral Implications of Covid-19 for Transplantation and Cellular Therapy Recipients
Authors: Knight Jennifer M. , Taylor Mallory R. , Rentscher Kelly E. , Henley Elisabeth C. , Uttley Hannah A. , Nelson Ashley M. , Turcotte Lucie M. , McAndrew Natalie S. , Amonoo Hermioni L. , Mohanraj Lathika , Kelly Debra Lynch , Costanzo Erin S.
Published: July 2022

A growing body of literature has emphasized the importance of biobehavioral processes – defined as the interaction of behavior, psychology, socioenvironmental factors, and biological processes – for clinical outcomes among transplantation and cellular therapy (TCT) patients. TCT recipients are especially vulnerable to distress associated with pandemic conditions and represent a notably immunocompromised group at greater risk for SARS-CoV-2 infection with substantially worse outcomes. The summation of both the immunologic and psychologic vulnerability of TCT patients renders them particularly susceptible to adverse biobehavioral sequelae associated with the Covid-19 pandemic. Stress and adverse psychosocial factors alter neural and endocrine pathways through sympathetic nervous system and hypothalamic-pituitary-adrenal axis signaling that ultimately affect gene regulation in immune cells. Reciprocally, global inflammation and immune dysregulation related to TCT contribute to dysregulation of neuroendocrine and central nervous system function, resulting in the symptom profile of depression, fatigue, sleep disturbance, and cognitive dysfunction. In this article, we draw upon literature on immunology, psychology, neuroscience, hematology and oncology, Covid-19 pathophysiology, and TCT processes to discuss how they may intersect to influence TCT outcomes, with the goal of providing an overview of the significance of biobehavioral factors in understanding the relationship between Covid-19 and TCT, now and for the future. We discuss the roles of depression, anxiety, fatigue, sleep, social isolation and loneliness, and neurocognitive impairment, as well as specific implications for sub-populations of interest, including pediatrics, caregivers, and TCT donors. Finally, we address protective psychological processes that may optimize biobehavioral outcomes affected by Covid-19.
The Impact of the First Year of the COVID-19 Pandemic on Canine Rabies Control Efforts: A Mixed-Methods Study of Observations About the Present and Lessons for the Future
Authors: Nadal Deborah , Abela-Ridder Bernadette , Beeching Sarah , Cleaveland Sarah , Cronin Katy , Steenson Rachel , Hampson Katie
Published: July 2022

Achieving zero human deaths from dog-mediated rabies has been set as a global target for 2030. However, the COVID-19 pandemic has disrupted essential health services across the world, with disproportionate impacts on Neglected Tropical Diseases. Through a mixed-method study using stakeholder questionnaires and in-depth interviews, we examined the scale and nature of disruption from the first year of the pandemic to rabies control programs, and reflected on lessons for the future. Study participants included practitioners and policymakers working in government, academia, international organizations, and the pharmaceutical industry across 48 countries, mainly in Africa and Asia. Mass dog vaccination, essential to rabies control, was most heavily impacted and in 2020, was carried out as planned in just 5% of surveyed countries. Access to post-exposure prophylaxis (PEP) also decreased due to fear of COVID-19 infection and difficulties in reaching health care centers. Dog vaccination and PEP delivery suffered from disruptions to the importation and distribution of vaccines. School closures affected rabies awareness activities and, when public events moved online, they could not reach the most disadvantaged groups. Surveillance, already weak, was severely disrupted by movement restrictions which, together with reduced demand for PEP, exacerbated under-reporting. Participants reported growing complaints around free-roaming dogs, with numbers likely to have increased in some settings. In some countries, dog rabies outbreaks and human rabies cases were already ascribed to the pandemic, but further impacts are likely still to be realized. Meanwhile, decreased demand for PEP from COVID-19 constraints could lead to reduced procurement in future. In the wake of post-COVID-19 demands on health services, there is an opportunity for veterinary services to show leadership in progressing the Zero by 30 agenda, particularly in scaling up mass dog vaccination within and across countries, as well as potential to make better use of community-based vaccinators. Countries must further secure stable procurement of dog and human vaccines, classifying them as essential goods prioritized for import and where needed, through sharing of stocks. Dedicated telemedicine services also show promise, for example through fostering participatory disease surveillance, including Integrated Bite Case Management, and delivering up-to-date instructions on the closest sources of PEP.
The Hippo Pathway Effector Transcriptional Co-activator With PDZ-Binding Motif Correlates With Clinical Prognosis and Immune Infiltration in Colorectal Cancer
Authors: Wang Yutong , Nie Hui , Li Huiling , Liao Zhiming , Yang Xuejie , He Xiaoyun , Ma Jian , Zhou Jianhua , Ou Chunlin
Published: July 2022

The transcriptional co-activator with PDZ-binding motif (TAZ) is a downstream effector of the Hippo pathway. It has been identified as an oncogene in certain tumor types; however, the function and role of TAZ in colorectal cancer (CRC) has not been illustrated. Here, we aimed to analyze the expression and role of TAZ in CRC. In this study, we investigated the expression level of TAZ in 127 CRC and matched adjacent normal tissues by immunohistochemistry (IHC) and analyzed its correlation with clinicopathological characteristics in CRC. Moreover, we further analyzed the role of TAZ in the CRC-associated immunology using integrative bioinformatic analyses. The cBioPortal and WebGestalt database were used to analyze the co-expressed genes and related pathways of TAZ in CRC by gene ontology (GO) and KEGG enrichment analyses. Meanwhile, the correlations between TAZ and the infiltrating immune cells and gene markers were analyzed by TIMER database. Our study revealed that TAZ expression is higher in CRC tissues than in matched adjacent non-tumor tissues. In addition, CRC patients with higher TAZ expression demonstrated poor overall survival (OS) and recurrent-free survival rates as compared to CRC patients with lower expression of TAZ. Furthermore, the TAZ expression was identified to closely associate with the immune infiltration of CD4 + T, CD8 + T, and B cells. Taken together, our findings suggest that TAZ may serve as a promising prognostic biomarker and therapeutic target in CRC.
Microbiome Heritability and Its Role in Adaptation of Hosts to Novel Resources
Authors: Bisschop Karen , Kortenbosch Hylke H. , van Eldijk Timo J. B. , Mallon Cyrus A. , Salles Joana F. , Bonte Dries , Etienne Rampal S.
Published: July 2022

Microbiomes are involved in most vital processes, such as immune response, detoxification, and digestion and are thereby elementary to organismal functioning and ultimately the host’s fitness. In turn, the microbiome may be influenced by the host and by the host’s environment. To understand microbiome dynamics during the process of adaptation to new resources, we performed an evolutionary experiment with the two-spotted spider mite, Tetranychus urticae. We generated genetically depleted strains of the two-spotted spider mite and reared them on their ancestral host plant and two novel host plants for approximately 12 generations. The use of genetically depleted strains reduced the magnitude of genetic adaptation of the spider mite host to the new resource and, hence, allowed for better detection of signals of adaptation via the microbiome. During the course of adaptation, we tested spider mite performance (number of eggs laid and longevity) and characterized the bacterial component of its microbiome (16S rRNA gene sequencing) to determine: (1) whether the bacterial communities were shaped by mite ancestry or plant environment and (2) whether the spider mites’ performance and microbiome composition were related. We found that spider mite performance on the novel host plants was clearly correlated with microbiome composition. Because our results show that only little of the total variation in the microbiome can be explained by the properties of the host (spider mite) and the environment (plant species) we studied, we argue that the bacterial community within hosts could be valuable for understanding a species’ performance on multiple resources.
Blood Pressure Changes During Mechanical Thrombectomy for Acute Ischemic Stroke Are Associated With Serious Early Treatment Complications: Symptomatic Intracerebral Hemorrhage and Malignant Brain Edema
Authors: Wiącek Marcin , Szymański Maciej , Walewska Klaudia , Bartosik-Psujek Halina
Published: July 2022

Background

Symptomatic intracranial hemorrhage (sICH) and malignant brain edema (MBE) are well-known deleterious endovascular treatment (EVT) complications that some studies found to be associated with postprocedural blood pressure (BP) variability. We aimed to evaluate their association with periprocedural BP changes, including its intraprocedural decrease.

Methods

We retrospectively analyzed the data of 132 consecutive patients that underwent EVT between 1 December 2018 and 31 December 2019, for anterior circulation ischemic stroke. Analyzed predictors of sICH and MBE included non-invasively obtained BP before and 5-min after treatment, intraprocedural relative decreases of BP from baseline, and its post-treatment increases. SICH was defined in accordance with the Safe Implementation of Thrombolysis in Stroke-Monitoring Study (SITS-MOST) criteria and MBE as brain edema with midline shift on the follow-up imaging. We used binary logistic regression analysis to investigate the association of BP parameters and the incidence of sICH and MBE.

Results

Among the included patients, 11 (8.3%) developed sICH and 31 (23.5%) MBE. The intraprocedural decrease of mean arterial blood pressure (MAP) was independently associated with MBE occurrence (aOR per 10 mmHg drop from baseline 1.27; 95% CI 1.01–1.60; P = 0.040). Over 40% MAP drop was associated with a higher risk of sICH in the entire cohort (aOR 4.24; 95% CI 1.33–13.51; P = 0.015), but not in the subgroup with successful reperfusion (aOR 2.81; 95% CI 0.64–12.23; P = 0.169). Post-treatment systolic blood pressure (SBP) and MAP elevation above their minimal values during MT are significantly associated with the development of sICH (aOR per 10 mmHg SBP increase 1.78; 95% CI 1.15–2.76; P = 0.010 and aOR per 10 mmHg MAP increase 1.78; 95% CI 1.04–3.03; P = 0.035).

Conclusions

In the anterior circulation ischemic stroke patients relative MAP decrease during EVT is associated with a higher risk of MBE occurrence, and over 40% MAP drop with a higher incidence of both MBE and sICH. Post-treatment elevation of SBP and MAP increased the risk of sICH.
Optimal Cutoffs for the Diagnosis of Sarcopenia in Older Chinese Adults
Authors: Ge Sheng , Du Qin , Feng Xiaohui , Liu Yan , Wang Hui , Hai Shan , Shi Xiaodong , Sun Wenguang , Ma Aiqin , Lv Tingting , Liu Haili , Pinupa Venkata Saibaba , Yalawar Menaka , Baggs Geraldine E. , Dong Birong , Chen Wei
Published: July 2022

Background

The optimal criteria for sarcopenia in the older Chinese population have not been defined. Consequently, this study aims to determine the optimal cutoffs of grip strength, appendicular skeletal muscle index (ASMI) using bioelectrical impedance analysis (BIA), and gait speed, comprising the best definition of sarcopenia for older Chinese populations.

Methods

A total of 2,821 (1,398 men and 1,423 women) community-dwelling older people (≥60 years) and 409 (205 men and 204 women) young healthy adults (25–34 years) were recruited from three big cities in China. Besides gait speed and grip strength, we examined ASMI by BIA and dual-energy X-ray absorptiometry (DXA), comprising the three components of sarcopenia. DXA classification for low ASMI, 20th percentile among older adults in the study sample, was found to be best compared with the other existing classification, 1 SD and 2 SD below the mean for the young population, and was used as the gold standard to determine the optimal cutoffs of BIA using receiver operating characteristic curves (ROC). The cutoffs of handgrip strength and gait speed were determined following the same rule.

Results

Using gender-specific 20th percentiles of DXA (6.53 kg/m2 for men and 5.40 kg/m2 for women), the cutoffs 7.05 kg/m2 for men and 5.85 kg/m2 for women were determined as optimal cutoffs of BIA by achieving the largest sensitivity (0.81, 95% CI: 0.63–0.93 for men and 0.90, 95% CI: 0.73–0.98 for women) and specificity greater than 0.80 (0.80, 95% CI: 0.72–0.87 for men and 0.81, 95% CI: 0.72–0.87 for women) in the ROC analysis. The 28.5 kg and 1.05 m/s for men and 18.6 kg and 1.01 m/s for women were determined as the cutoffs for handgrip strength and gait speed, respectively. Based on the derived cutoffs, 14.2% of men and 15.7% of women in the older Chinese study population were classified as sarcopenia.

Conclusion

Notably, 7.05 kg/m2, 28.5 kg, and 1.05 m/s for men and 5.85 kg/m2, 18.6 kg, and 1.01 m/s for women were selected as the optimal cutoffs for low ASMI by BIA, handgrip strength, and gait speed, respectively. These optimal cutoffs will enhance practicability for screening sarcopenia in primary care and clinical settings.
Genotypes of Papillary Thyroid Carcinoma With High Lateral Neck Metastasis in Chinese Population
Authors: Guo Wei , Huang Junwei , Shi Taiping , Duan Hanyuan , Chen Xiaohong , Huang Zhigang
Published: July 2022

Papillary Thyroid Carcinoma (PTC) is one of the most commonly diagnosed cancer types in China, characterized by its early age at diagnosis and high 25-year survival rate. Distinct mutational patterns in PTC have been linked to activation of the mitogen-activated protein kinase (MAPK) signaling pathway. To explore the clinical significance of genomic variation patterns in Chinese patients with thyroid carcinoma, we investigated the genomic variants in 83 PTC cases with complete clinical records. The mutational patterns were evaluated using a 688-gene panel which covered known driver genes in PTC tumorigenesis, and featured genetic markers in various PTC-related pathways. We evaluated the relationship between mutational landscape and various clinical information in PTC patients with lateral lymph node metastasis. BRAF V600E was the most common mutation. Mutations in NF1, CDC27, PMS2 and PPP4R2 were more common in men, and mutations in NF1, PMS2 and TERT were related to lateral lymph node metastasis. According to the clustering of mutational patterns, we show that the underline driving mechanisms in lateral lymph node metastasis can be divided into two major groups (BRAF-TERT pathway, and NF1-PMS2 pathway). When combined with the TERT mutations, the BRAF mutation group was prone to lateral lymph node metastasis, particularly in elderly women. The NF1 mutations usually co-existed with PMS2 mutations, and this group included more men and young patients who had a high tumor mutational burden and lateral lymph node metastasis rate.
Drug Resistance Mechanisms of Acute Myeloid Leukemia Stem Cells
Authors: Niu Jialan , Peng Danyue , Liu Lingbo
Published: July 2022

Acute myeloid leukemia (AML) is a polyclonal and heterogeneous hematological malignancy. Relapse and refractory after induction chemotherapy are still challenges for curing AML. Leukemia stem cells (LSCs), accepted to originate from hematopoietic stem/precursor cells, are the main root of leukemogenesis and drug resistance. LSCs are dynamic derivations and possess various elusive resistance mechanisms. In this review, we summarized different primary resistance and remolding mechanisms of LSCs after chemotherapy, as well as the indispensable role of the bone marrow microenvironment on LSCs resistance. Through a detailed and comprehensive review of the spectacle of LSCs resistance, it can provide better strategies for future researches on eradicating LSCs and clinical treatment of AML.
CircRNA-Associated CeRNAs Regulatory Axes in Retinoblastoma: A Systematic Scoping Review
Authors: Asadi Mohammad Reza , Moslehian Marziyeh Sadat , Sabaie Hani , Sharifi-Bonab Mirmohsen , Hakimi Parvin , Hussen Bashdar Mahmud , Taheri Mohammad , Rakhshan Azadeh , Rezazadeh Maryam
Published: July 2022

Retinoblastoma (RB) is one of the most common childhood cancers caused by RB gene mutations (tumor suppressor gene in various patients). A better understanding of molecular pathways and the development of new diagnostic approaches may lead to better treatment for RB patients. The number of studies on ceRNA axes is increasing, emphasizing the significance of these axes in RB. Circular RNAs (circRNAs) play a vital role in competing endogenous RNA (ceRNA) regulatory axes by sponging microRNAs and regulating gene expression. Because of the broadness of ceRNA interaction networks, they may assist in investigating treatment targets in RB. This study conducted a systematic scoping review to evaluate verified loops of ceRNA in RB, focusing on the ceRNA axis and its relationship to circRNAs. This scoping review was carried out using a six-step strategy and the Prisma guideline, and it involved systematically searching the publications of seven databases. Out of 363 records, sixteen articles were entirely consistent with the defined inclusion criteria and were summarized in the relevant table. The majority of the studies focused on the circRNAs circ_0000527, circ_0000034, and circTET1, with approximately two-fifths of the studies focusing on a single circRNA. Understanding the many features of this regulatory structure may help elucidate RB’s unknown causative factors and provide novel molecular potential therapeutic targets and medical fields.
Long-Term Responders After Autologous Stem Cell Transplantation in Multiple Myeloma
Authors: Oliver-Caldes Aina , Soler-Perromat Juan Carlos , Lozano Ester , Moreno David , Bataller Alex , Mozas Pablo , Garrote Marta , Setoain Xavier , Aróstegui Juan Ignacio , Yagüe Jordi , Tovar Natalia , Jiménez Raquel , Rodríguez-Lobato Luis Gerardo , Cibeira M. Teresa , Rosiñol Laura , Bladé Joan , Juan Manel , Fernández de Larrea Carlos
Published: July 2022

Introduction

Multiple myeloma (MM) is considered an incurable hematological neoplasm. For transplant-eligible patients, initial treatment includes an induction phase followed by an autologous stem cell transplantation (ASCT). Despite the introduction of several drugs in the past years, relapses still occur. Nevertheless, some patients achieve sustained responses after successful induction treatment and ASCT.

Methods

We retrospectively evaluated all patients diagnosed with MM in our institution who underwent induction treatment and ASCT between 1990 and 2015. The subset of patients who achieved a sustained response (any degree) for 5 or more years after ASCT without further treatment or signs of progression were distinguished as “long-term responders” (LTRs). In the non-LTR group, a cohort referred to as “prolonged responders” (PLRs) showed sustained response of at least 5 years after ASCT but eventually relapsed. We collected and analyzed clinical and laboratory data.

Results

Two hundred and fifty patients were diagnosed with MM and received induction treatment and ASCT at our institution in the study period. Among them, 54 (21.6%) patients met the criteria for LTR. Some diagnostic features such as a younger age, female gender, ECOG performance status of 0, lower International Staging System (ISS) stage, lower bone marrow plasma cell infiltration, and lower serum levels of calcium, C-reactive protein, and lactate dehydrogenase (LDH) were found to be more prevalent in LTR. Female gender, an ECOG performance status of 0, a localized Durie-Salmon stage, an ISS of I–II, the absence of bone disease, and an LDH within normal range were also predictive of longer progression-free survival (PFS) and overall survival (OS) in the whole cohort. The depth of the response achieved after induction and ASCT as well as the administration of an IMID-based maintenance regimen may play a role in the differences observed on PFS between cohorts. A detectable M-protein with a monoclonal gammopathy of undetermined significance (MGUS)-like behavior was detected in one-third of LTR after ASCT. Although relapses continue to occur in patients who achieve a 5-year treatment-free period after ASCT, a plateau is observed in the survival curves at approximately 21 years of follow-up.
Sensory Perception Quotient Reveals Visual, Scent and Touch Sensory Hypersensitivity in People With Fibromyalgia Syndrome
Authors: Dorris Emma R. , Maccarthy James , Simpson Ken , McCarthy Geraldine M.
Published: July 2022

Background

Environmental sensitivity is commonly reported by people with fibromyalgia syndrome. People living with fibromyalgia syndrome frequently report hypersensitivity to noxious and non-noxious sensations. To date, there has been little empirical validation of sensory disturbance to non-noxious triggers. Environmental sensitivity is used as a diagnostic feature only in Bennet's alternative criteria for diagnosis of fibromyalgia, where it was ranked the second most important of the components for diagnosis, after number of pain sites. The aim of this study was to use a validated sensory measure to determine if people with fibromyalgia have greater sensory disturbances compared to people with other chronic pain conditions.

Methods

This study used the Sensory Perception Quotient (SPQ) 92 question survey in adults with chronic pain conditions. A fibromyalgia group (n = 135) and a non-fibromyalgia chronic pain control group (n = 45) were recruited. All participants completed the SPQ as a self-report measure of sensory processing. In addition to the original SPQ scoring method, the Revised Scoring of the Sensory Perception Quotient (SPQ-RS) method was used to investigate self-reported hypersensitivity and hyposensitivity and the vision, hearing, taste, touch, and smell subscales. Chi-squared tests were used for categorical variables and Mann Whitney U, or Kruskal-Wallis H test were used to compare groups.

Results

The fibromyalgia group reported significantly more sensitivity compared to the control group (p = 0.030). The fibromyalgia group reported significantly greater hypersensitivity (p = 0.038), but not more hyposensitivity (p = 0.723) compared to controls. The average fibromyalgia SPQ score (92.64 ± 23.33) was similar to that previously reported for adults with autism (92.95 ± 26.61). However, whereas adults with autism had broad range hypersensitivity, the fibromyalgia group reported significantly more hypersensitivity compared to the control group, but the range was restricted to vision (p = 0.033), smell (p = 0.049) and touch (0.040).

Conclusions

These findings demonstrate greater sensory hypersensitivity in people with fibromyalgia compared to people with other chronic pain disorders. Greater hypersensitivity was restricted to touch, vision, and smell, all of which have previously been demonstrated to crosstalk with nociception.
Association Between Language Use and ICU Transfer and Serious Adverse Events in Hospitalized Pediatric Patients Who Experience Rapid Response Activation
Authors: McDade Jessica E. , Olszewski Aleksandra E. , Qu Pingping , Ramos Jessica , Bell Shaquita , Adiele Alicia , Roberts Joan , Coker Tumaini R.
Published: July 2022

Background

Hospitalized patients and caregivers who use a language other than English have worse health outcomes, including longer length of stay, more frequent readmissions, and increased rates of in-hospital adverse events. Children who experience clinical deterioration (as measured by a Rapid Response Team event) during a hospitalization are at increased risk for adverse events and mortality.

Methods

We describe the results of a retrospective cohort study using hospital records at a free-standing, quaternary children's hospital, to examine the association of language of care with outcomes (transfer to intensive care, adverse event, mortality prior to discharge) following Rapid Response Team event, and whether increased interpreter use among patients who use a language other than English is associated with improved outcomes following Rapid Response Team event.

Results

In adjusted models, Rapid Response Team events for patients who use a language other than English were associated with higher transfer rates to intensive care (RR 1.1, 95% CI 1.01, 1.21), but not with adverse event or mortality. Among patients who use a language other than English, use of 1-2 interpreted sessions per day was associated with lower transfer rates to intensive care compared to use of less than one interpreted session per day (RR 0.79, 95% 0.66, 0.95).

Conclusion

Rapid Response Team events for hospitalized children of families who use a language other than English are more often followed by transfer to intensive care, compared with Rapid Response Team events for children of families who use English. Improved communication with increased interpreter use for hospitalized children who use a language other than English may lead to improvements in Rapid Response Team outcomes.
Intrinsic Differences in Immune Checkpoint Inhibitor-Induced Myocarditis: A Retrospective Analysis of Real World Data
Authors: Lei Yanna , Zheng Xiufeng , Huang Qian , Li Xiaoying , Qiu Meng , Liu Ming
Published: July 2022

Immune-related myocarditis is a severe and even life-threatening immune-related adverse event (irAE) which may also be underestimated due to the challenge in diagnosis. The inherent difference between individuals with immune-associated myocarditis has received little attention. Our study aimed to identify which baseline characteristics could contribute to distinguishing mild from severe ICI myocarditis. A retrospective analysis was conducted between March 2019 and June 2020 in West China Hospital, and 18 patients with immune-related myocarditis were studied. Patients were classified as having mild (n = 12) or severe myocarditis (n = 6), according to the clinical manifestations and hemodynamic complications. Factors associated with severe myocarditis were identified by comparing covariates derived from medical records in various groups. In this retrospective analysis, the median age of the 18 patients was 60 years old. Most myocarditis cases occur early and approximately after the first or second ICI infusion. The severity of myocarditis may be correlated with lactate dehydrogenase (LDH) (p = 0.04) and troponin levels (p = 0.0057). The relationship between troponin and myocarditis was further confirmed in another cohort, which included 30 patients. In addition, patients are more likely to develop multi-irAEs, and myositis was the most common second irAE. Those who experience multi-irAEs usually had significantly higher LDH (p = 0.02) and myoglobin levels (p = 0.02) than those who did not experience them. All patients were treated with steroids timely, and the mortality rate was 5.6% in our study. In this study, we explored risk factors for severe myocarditis and emphasized the importance of a multidisciplinary team in assisting diagnosis and treatment options. It is critical to initiate corticosteroid therapy, regardless of the severity of the myocarditis.
HIF-1α in Osteoarthritis: From Pathogenesis to Therapeutic Implications
Authors: Zeng Chu-Yang , Wang Xi-Feng , Hua Fu-Zhou
Published: July 2022

Osteoarthritis is a common age-related joint degenerative disease. Pain, swelling, brief morning stiffness, and functional limitations are its main characteristics. There are still no well-established strategies to cure osteoarthritis. Therefore, better clarification of mechanisms associated with the onset and progression of osteoarthritis is critical to provide a theoretical basis for the establishment of novel preventive and therapeutic strategies. Chondrocytes exist in a hypoxic environment, and HIF-1α plays a vital role in regulating hypoxic response. HIF-1α responds to cellular oxygenation decreases in tissue regulating survival and growth arrest of chondrocytes. The activation of HIF-1α could regulate autophagy and apoptosis of chondrocytes, decrease inflammatory cytokine synthesis, and regulate the chondrocyte extracellular matrix environment. Moreover, it could maintain the chondrogenic phenotype that regulates glycolysis and the mitochondrial function of osteoarthritis, resulting in a denser collagen matrix that delays cartilage degradation. Thus, HIF-1α is likely to be a crucial therapeutic target for osteoarthritis via regulating chondrocyte inflammation and metabolism. In this review, we summarize the mechanism of hypoxia in the pathogenic mechanisms of osteoarthritis, and focus on a series of therapeutic treatments targeting HIF-1α for osteoarthritis. Further clarification of the regulatory mechanisms of HIF-1α in osteoarthritis may provide more useful clues to developing novel osteoarthritis treatment strategies.