bluuebunny/crossref_metadata_2025 huggingface dataset:
The clinical spectrum and immunopathological mechanisms underlying ZIKV-induced neurological manifestations
Authors: Filgueiras Igor Salerno , Torrentes de Carvalho Amanda , Cunha Daniela Prado , Mathias da Fonseca Dennyson Leandro , El Khawanky Nadia , Freire Paula Paccielli , Cabral-Miranda Gustavo , Schimke Lena F. , Camara Niels Olsen Saraiva , Ochs Hans D. , Peron Jean Pierre Schatzmann , Cabral-Marques Otávio , de Vasconcelos Zilton Farias Meira
Published: August 2021

Since the 2015 to 2016 outbreak in America, Zika virus (ZIKV) infected almost 900,000 patients. This international public health emergency was mainly associated with a significant increase in the number of newborns with congenital microcephaly and abnormal neurologic development, known as congenital Zika syndrome (CZS). Furthermore, Guillain–Barré syndrome (GBS), a neuroimmune disorder of adults, has also been associated with ZIKV infection. Currently, the number of ZIKV-infected patients has decreased, and most of the cases recently reported present as a mild and self-limiting febrile illness. However, based on its natural history of a typical example of reemerging pathogen and the lack of specific therapeutic options against ZIKV infection, new outbreaks can occur worldwide, demanding the attention of researchers and government authorities. Here, we discuss the clinical spectrum and immunopathological mechanisms underlying ZIKV-induced neurological manifestations. Several studies have confirmed the tropism of ZIKV for neural progenitor stem cells by demonstrating the presence of ZIKV in the central nervous system (CNS) during fetal development, eliciting a deleterious inflammatory response that compromises neurogenesis and brain formation. Of note, while the neuropathology of CZS can be due to a direct viral neuropathic effect, adults may develop neuroimmune manifestations such as GBS due to poorly understood mechanisms. Antiganglioside autoantibodies have been detected in multiple patients with ZIKV infection–associated GBS, suggesting a molecular mimicry. However, further additional immunopathological mechanisms remain to be uncovered, paving the way for new therapeutic strategies.
Elimination of schistosomiasis in China: Current status and future prospects
Authors: Wang Wei , Bergquist Robert , King Charles H. , Yang Kun
Published: August 2021

Elimination of schistosomiasis as a public health problem among all disease-endemic countries in 2030 is an ambitious goal. Recent achievements resulting from mass drug administration (MDA) with praziquantel is promising but may need to be complemented with also other means. Schistosomiasis was highly prevalent in China before the initiation of the national schistosomiasis control program in the mid-1950s, and, at that time, the country bore the world’s highest burden of schistosomiasis. The concerted control efforts, upheld without interruption for more than a half century, have resulted in elimination of the disease as a public health problem in China as of 2015. Here, we describe the current status of schistosomiasis in China, analyze the potential challenges affecting schistosomiasis elimination, and propose the future research needs and priorities for the country, aiming to provide more universal insights into the structures needed for a global schistosomiasis elimination encompassing also other endemic regions.
Effect of comorbid mood and anxiety disorders on breast and cervical cancer screening in immune-mediated inflammatory disease
Authors: Marrie Ruth Ann , Walld Randy , Bolton James M. , Sareen Jitender , Patten Scott B. , Singer Alexander , Lix Lisa M. , Hitchon Carol A. , Marriott James J. , El-Gabalawy Renée , Katz Alan , Fisk John D. , Bernstein Charles N. ,   for the CIHR Team in Defining the Burden and Managing the Effects of Psychiatric Comorbidity in Chronic Immunoinflammatory Disease
Published: August 2021

We aimed to examine rates of breast and cervical cancer screening in women with immune-mediated inflammatory diseases (IMID), including inflammatory bowel disease (IBD), multiple sclerosis (MS) and rheumatoid arthritis (RA) versus a matched cohort with IMID; and examine the association of psychiatric comorbidity with screening in these populations. We conducted a retrospective cohort study in Manitoba, Canada using administrative data. We identified women with IBD, MS and RA, and controls without these IMID matched on age and region. Annually, we identified individuals with any active mood/anxiety disorder. Using physician claims, we determined the proportion of each cohort who had cervical cancer screening within three-year intervals, and mammography screening within two-year intervals. We modeled the difference in the proportion of the IMID and matched cohorts who underwent mammography; and pap tests using log-binomial regression with generalized estimating equations, adjusting for sociodemographics, comorbidity and immune therapy use. We tested for additive interactions between cohort and mood/anxiety disorder status. During 2006–2016, we identified 17,230 women with IMID (4,623 with IBD, 3,399 with MS, and 9,458 with RA) and 85,349 matched controls. Having an IMID was associated with lower (-1%) use of mammography; however, this reflected a mixture of more mammography in the IBD cohort (+2.9%) and less mammography in the MS (-4.8 to -5.2%) and RA (-1.5%) cohorts. Within the IBD, MS and RA cohorts, having an active mood/anxiety disorder was associated with more mammography use than having an inactive mood/anxiety disorder. The MS and RA cohorts were less likely to undergo Pap testing than their matched cohorts. In the absence of an active mood/anxiety disorder, the IBD cohort was more likely to undergo Pap testing than its matched cohort; the opposite was true when an active mood/anxiety disorder was present. Among women with an IMID, mood/anxiety disorder influence participation in cancer screening.
Fusarium graminearum DICER-like-dependent sRNAs are required for the suppression of host immune genes and full virulence
Authors: Werner Bernhard Timo , Koch Aline , Šečić Ena , Engelhardt Jonas , Jelonek Lukas , Steinbrenner Jens , Kogel Karl-Heinz
Published: August 2021

In filamentous fungi, gene silencing by RNA interference (RNAi) shapes many biological processes, including pathogenicity. Recently, fungal small RNAs (sRNAs) have been shown to act as effectors that disrupt gene activity in interacting plant hosts, thereby undermining their defence responses. We show here that the devastating mycotoxin-producing ascomyceteFusarium graminearum(Fg) utilizes DICER-like (DCL)-dependent sRNAs to target defence genes in two Poaceae hosts, barley (Hordeum vulgare,Hv) andBrachypodium distachyon(Bd). We identified 104Fg-sRNAs with sequence homology to host genes that were repressed during interactions ofFgandHv, while they accumulated in plants infected by the DCL double knock-out (dKO) mutant PH1-dcl1/2. The strength of target gene expression correlated with the abundance of the correspondingFg-sRNA. Specifically, the abundance of three tRNA-derived fragments (tRFs) targeting immunity-relatedEthylene overproducer 1-like 1(HvEOL1)and three Poaceae orthologues ofArabidopsis thaliana BRI1-associated receptor kinase 1(HvBAK1,HvSERK2andBdSERK2) was dependent on fungal DCL. Additionally, RNA-ligase-mediated Rapid Amplification of cDNA Ends (RLM-RACE) identified infection-specific degradation products for the three barley gene transcripts, consistent with the possibility that tRFs contribute to fungal virulence via targeted gene silencing.
Prospective cohort study of exposure to tobacco imagery in popular films and smoking uptake among children in southern India
Authors: Kulkarni Muralidhar M. , Kamath Asha , Kamath Veena G. , Lewis Sarah , Bogdanovica Ilze , Bains Manpreet , Cranwell Jo , Fogarty Andrew , Arora Monika , Nazar Gaurang P. , Ballal Kirthinath , Naik Ashwath K. , Bhagawath Rohith , Britton John
Published: August 2021

Background

Exposure to tobacco imagery in films causes young people to start smoking. Popular Indian films contain high levels of tobacco imagery, but those that do are required by law to display onscreen health warnings when smoking imagery occurs and to include other health promotion messaging before and during the film. We report a prospective cohort study of incident smoking in relation to exposure to film tobacco imagery and anti-tobacco messaging in a cohort of children in southern India.

Methods

We carried out a one-year longitudinal follow up questionnaire survey in 2018 of a cohort of 39,282 students in grades 6, 7 and 8 (aged between 10 and 15 years) in schools in the Udupi district of Karnataka State in India who participated in a 2017 cross-sectional study of exposure to smoking in films and ever smoking status.

Results

We obtained usable linked data in 2018 from 33,725 of the 39,282 (86%) participants with data from 2017. Incident smoking was reported by 382 (1.1%) participants. After adjusting for age, sex and common confounders significantly associated with incident smoking there was no significant independent effect of exposure to film smoking, either as a binary (Odds Ratio 1.6, 95% Confidence Interval (CI) 0.5 to 4.9) or as a graded variable, on smoking uptake. An exploratory analysis indicated that the presence of on-screen health warnings that complied fully with Indian law was associated with a significantly lower odds of smoking uptake (Odds Ratio 0.8 (0.6 to 1.0, p = 0.031) relative to the same exposure sustained in absence of compliant warnings.

Conclusion

Exposure to tobacco imagery in Indian films was not associated with a significantly increased risk of incident smoking in South Indian children. While it is possible that this finding is a false negative, it is also possible that the effect of film exposure has been attenuated by the presence of on-screen health warnings or other Indian tobacco-free film rules. Our findings therefore support the wider implementation of similar tobacco-free film measures in other countries.
Non-territorial GPS-tagged golden eagles Aquila chrysaetos at two Scottish wind farms: Avoidance influenced by preferred habitat distribution, wind speed and blade motion status
Authors: Fielding Alan H. , Anderson David , Benn Stuart , Dennis Roy , Geary Matthew , Weston Ewan , Whitfield D. Philip
Published: August 2021

Wind farms can have two broad potential adverse effects on birds via antagonistic processes: displacement from the vicinity of turbines (avoidance), or death through collision with rotating turbine blades. These effects may not be mutually exclusive. Using detailed data from 99 turbines at two wind farms in central Scotland and thousands of GPS-telemetry data from dispersing golden eagles, we tested three hypotheses. Before-and-after-operation analyses supported the hypothesis of avoidance: displacement was reduced at turbine locations in more preferred habitat and with more preferred habitat nearby. After-operation analyses (i.e. from the period when turbines were operational) showed that at higher wind speeds and in highly preferred habitat eagles were less wary of turbines with motionless blades: rejecting our second hypothesis. Our third hypothesis was supported, since at higher wind speeds eagles flew closer to operational turbines; especially–once more–turbines in more preferred habitat. After operation, eagles effectively abandoned inner turbine locations, and flight line records close to rotor blades were rare. While our study indicated that whole-wind farm functional habitat loss through avoidance was the substantial adverse impact, we make recommendations on future wind farm design to minimise collision risk further. These largely entail developers avoiding outer turbine locations which are in and surrounded by swathes of preferred habitat. Our study illustrates the insights which detailed case studies of large raptors at wind farms can bring and emphasises that the balance between avoidance and collision can have several influences.
Impaired memory B-cell recall responses in the elderly following recurrent influenza vaccination
Authors: Abreu Rodrigo B. , Kirchenbaum Greg A. , Sautto Giuseppe A. , Clutter Emily F. , Ross Ted M.
Published: August 2021

Influenza is a highly contagious viral respiratory disease that affects million of people worldwide each year. Annual vaccination is recommended by the World Health Organization with the goal of reducing influenza severity and limiting transmission through elicitation of antibodies targeting the hemagglutinin (HA) glycoprotein. The antibody response elicited by current seasonal influenza virus vaccines is predominantly strain-specific, but pre-existing influenza virus immunity can greatly impact the serological antibody response to vaccination. However, it remains unclear how B cell memory is shaped by recurrent annual vaccination over the course of multiple seasons, especially in high-risk elderly populations. Here, we systematically profiled the B cell response in young adult (18–34 year old) and elderly (65+ year old) vaccine recipients that received annual split inactivated influenza virus vaccination for 3 consecutive seasons. Specifically, the antibody serological and memory B-cell compartments were profiled for reactivity against current and historical influenza A virus strains. Moreover, multiparametric analysis and antibody landscape profiling revealed a transient increase in strain-specific antibodies in the elderly, but with an impaired recall response of pre-existing memory B-cells, plasmablast (PB) differentiation and long-lasting serological changes. This study thoroughly profiles and compares the immune response to recurrent influenza virus vaccination in young and elderly participants unveiling the pitfalls of current influenza virus vaccines in high-risk populations.
Constructing robust and efficient experimental designs in groundwater modeling using a Galerkin method, proper orthogonal decomposition, and metaheuristic algorithms
Authors: Ushijima Timothy T. , Yeh William W. G. , Wong Weng Kee
Published: August 2021

Estimating parameters accurately in groundwater models for aquifers is challenging because the models are non-explicit solutions of complex partial differential equations. Modern research methods, such as Monte Carlo methods and metaheuristic algorithms, for searching an efficient design to estimate model parameters require hundreds, if not thousands of model calls, making the computational cost prohibitive. One method to circumvent the problem and gain valuable insight on the behavior of groundwater is to first apply a Galerkin method and convert the system of partialdifferential equations governing the flow to a discrete problem and then use a Proper Orthogonal Decomposition to project the high-dimensional model space of the original groundwater model to create a reduced groundwater model with much lower dimensions. The reduced model can be solved several orders of magnitude faster than the full model and able to provide an accurate estimate of the full model. The task is still challenging because the optimization problem is non-convex, non-differentiable and there are continuous variables and integer-valued variables to optimize. Following convention, heuristic algorithms and a combination is used search to find efficient designs for the reduced groundwater model using various optimality criteria. The main goals are to introduce new design criteria and the concept of design efficiency for experimental design research in hydrology. The two criteria have good utility but interestingly, do not seem to have been implemented in hydrology. In addition, design efficiency is introduced. Design efficiency is a method to assess how robust a design is under a change of criteria. The latter is an important issue because the design criterion may be subjectively selected and it is well known that an optimal design can perform poorly under another criterion. It is thus desirable that the implemented design has relatively high efficiencies under a few criteria. As applications, two heuristic algorithms are used to find optimal designs for a small synthetic aquifer design problem and a design problem for a large-scale groundwater model and assess their robustness properties to other optimality criteria. The results show the proof of concept is workable for finding a more informed and efficient model-based design for a water resource study.
Eco-hydrology as a driver for tidal restoration: Observations from a Ramsar wetland in eastern Australia
Authors: Glamore William , Rayner Duncan , Ruprecht Jamie , Sadat-Noori Mahmood , Khojasteh Danial
Published: August 2021

Land reclamation projects and the installation of drainage infrastructure has impacted coastal wetlands worldwide. By altering water levels and inundation extent, these activities have changed the viable ecosystems onsite and resulted in the proliferation of freshwater species. As more than 50% of tidal wetlands have been degraded globally over the last 100 years, the importance of this issue is increasingly being recognised and tidal wetland restoration projects are underway worldwide. However, there are currently limited sites where large-scale reintroduction of tidal flushing has been implemented with the explicit aim to foster the growth of a threatened ecosystem. In this study, the tidal restoration of an internationally recognised Ramsar listed wetland in eastern Australia is described to highlight how coastal saltmarsh can be targeted by mimicking inundation depths and hydroperiod across the 410-ha site. Coastal saltmarsh is particularly important to this site as it is part of the east Australasian flyway for migratory birds and the minimum saltmarsh extent, as listed within the Ramsar’s limits of acceptable change, have been breached. To recreate coastal saltmarsh habitat onsite, water level and hydroperiod criteria were established based on similar vegetation patterns within the adjacent estuary. A calibrated 2D hydrodynamic model of the site was then used to test how the preferred inundation criteria could be applied to the largest possible restored wetland area. Once optimised, a synthetic tidal signal was implemented onsite via automated hydraulic controls. The onsite vegetation response over an 8-year period was assessed to highlight the ecosystem response to controlled tidal inundation and denoted substantial saltmarsh expansion during the period. The techniques applied onsite have successfully met the restoration targets and can be applied at similar sites worldwide, offsetting sea level rise impacts to natural inundation hydroperiod.
Does eye-tracking have an effect on economic behavior?
Authors: Kee Jennifer , Knuth Melinda , Lahey Joanna N. , Palma Marco A.
Published: August 2021

Eye-tracking is becoming an increasingly popular tool for understanding the underlying behavior driving human decisions. However, an important unanswered methodological question is whether the use of an eye-tracking device itself induces changes in participants’ behavior. We study this question using eight popular games in experimental economics chosen for their varying levels of theorized susceptibility to social desirability bias. We implement a simple between-subject design where participants are randomly assigned to either a control or an eye-tracking treatment. In seven of the eight games, eye-tracking did not produce different outcomes. In the Holt and Laury risk assessment (HL), subjects with multiple calibration attempts demonstrated more risk averse behavior in eye-tracking conditions. However, this effect only appeared during the first five (of ten) rounds. Because calibration difficulty is correlated with eye-tracking data quality, the standard practice of removing participants with low eye-tracking data quality resulted in no difference between the treatment and control groups in HL. Our results suggest that experiments may incorporate eye-tracking equipment without inducing changes in the economic behavior of participants, particularly after observations with low quality eye-tracking data are removed.
Preterm birth is not associated with asymptomatic/mild SARS-CoV-2 infection per se: Pre-pregnancy state is what matters
Authors: Cosma Stefano , Carosso Andrea Roberto , Cusato Jessica , Borella Fulvio , Carosso Marco , Gervasoni Fiammetta , Stura Ilaria , Preti Mario , Ghisetti Valeria , Di Perri Giovanni , Benedetto Chiara
Published: August 2021

Evidence for the real impact of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection on preterm birth is unclear, as available series report composite pregnancy outcomes and/or do not stratify patients according to disease severity. The purpose of the research was to determine the real impact of asymptomatic/mild SARS-CoV-2 infection on preterm birth not due to maternal respiratory failure. This case-control study involved women admitted to Sant Anna Hospital, Turin, for delivery between 20 September 2020 and 9 January 2021. The cumulative incidence of Coronavirus disease-19 was compared between preterm birth (case group, n = 102) and full-term delivery (control group, n = 127). Only women with spontaneous or medically-indicated preterm birth because of placental vascular malperfusion (pregnancy-related hypertension and its complications) were included. Current or past SARS-CoV-2 infection was determined by nasopharyngeal swab testing and detection of IgM/IgG antibodies in blood samples. A significant difference in the cumulative incidence of Coronavirus disease-19 between the case (21/102, 20.5%) and the control group (32/127, 25.1%) (P= 0.50) was not observed, although the case group was burdened by a higher prevalence of three known risk factors (body mass index > 24.9, asthma, chronic hypertension) for severe Coronavirus disease-19. Logistic regression analysis showed that asymptomatic/mild SARS-CoV-2 infection was not an independent predictor of spontaneous and medically-indicated preterm birth due to pregnancy-related hypertension and its complications (0.77; 95% confidence interval, 0.41-1.43). Pregnant patients without comorbidities need to be reassured that asymptomatic/mild SARS-CoV-2 infection does not increase the risk of preterm delivery. Preterm birth and severe Coronavirus disease-19 share common risk factors (i.e., body mass index > 24.9, asthma, chronic hypertension), which may explain the high rate of indicated preterm birth due to maternal conditions reported in the literature.
The effects of evidence-based expert testimony on perceptions of child sexual abuse involving recantation
Authors: Denne Emily , Stolzenberg Stacia N. , Neal Tess M. S.
Published: August 2021

Child sexual abuse (CSA) cases involving recantation invoke concerns about children’s reliability. Expert testimony can help explain the complexities of these cases. Experts have historically relied on Child Sexual Abuse Accommodation Syndrome (CSAAS), yet this is not science-based. In a CSA case involving recantation, how would evidence-based testimony affect perceptions of child credibility when compared to CSAAS? Across 2 studies, we test the effects of expert testimony based on evidence-based science, nonscientific evidence, and experience-based evidence on outcomes in CSA cases involving recantation. Evidence-based testimony led to higher perceptions of credibility and scientific rigor of the evidence when compared to CSAAS testimony. Evidence-based testimony also led to more guilty verdicts when compared to the control. In sum, jurors had some ability to detect evidence strength, such that evidence-based expert testimony was superior to CSAAS testimony in many respects, and consistently superior to experience-based testimony in these cases.
A new fatigue damage model for pavement concrete beams bearing multi-level bending loads
Authors: Lei Liang , Xingang Shi , Yunhua Cui , Lefan Wang , Xiangcheng Yan
Published: August 2021

MTS-810 material testing machine and acoustic emission signal analyzer were adopted to explore the mechanical behavior of concrete beams broken by the static load and the nonlinear cumulative damage law of concrete beams broken by fatigue bending from single-stage loading. Then, by introducing the Ramesh Talreja’s Damage Criterion, the damage rule of single-stage loading was extended to the damage accumulation rule under multi-stage loading, and the results were verified by the results of two-stage and three-stage fatigue loading tests. Two main conclusions are achieved: first, affected by four-point bending load, the fatigue life of the concrete specimen is in line with the law of the two-parameter Weibull distribution, namely the higher the stress level is, the shorter the fatigue life is. Second, an obvious nonlinear relationship was discovered in the damage of concrete. The model deduced in this paper and the Palmgren-Miner linear damage accumulation model were adopted to compare the test results of flexural fatigue under single, two and three stage loads. The calculation results of this model were more reliable.
Genome-wide association study of trypanosome prevalence and morphometric traits in purebred and crossbred Baoulé cattle of Burkina Faso
Authors: Yougbaré Bernadette , Soudré Albert , Ouédraogo Dominique , Zoma Bienvenue L. , Tapsoba Arnaud S. R. , Sanou Moumouni , Ouédraogo-Koné Salifou , Burger Pamela A. , Wurzinger Maria , Khayatzadeh Negar , Tamboura Hamidou H. , Mwai Okeyo Ally , Traoré Amadou , Sölkner Johann , Mészáros Gábor
Published: August 2021

In this study, single-SNP GWAS analyses were conducted to find regions affecting tolerance against trypanosomosis and morphometrics traits in purebred and crossbred Baoulé cattle of Burkina Faso. The trypanosomosis status (positive and negative) and a wide set of morphological traits were recorded for purebred Baoulé and crossbred Zebu x Baoulé cattle, and genotyped with the Illumina Bovine SNP50 BeadChip. After quality control, 36,203 SNPs and 619 animals including 343 purebred Baoulé and 279 crossbreds were used for the GWAS analyses. Several important genes were found that can influence morphological parameters. Although there were no genes identified with a reported strong connection to size traits, many of them were previously identified in various growth-related studies. A re-occurring theme for the genes residing in the regions identified by the most significant SNPs was pleiotropic effect on growth of the body and the cardiovascular system. Regarding trypanosomosis tolerance, two potentially important regions were identified in purebred Baoulé on chromosomes 16 and 24, containing the CFH, CRBN, TRNT1 and, IL5RA genes, and one additional genomic region in Baoulé, x Zebu crossbreds on chromosome 5, containing MGAT4C and NTS. Almost all of these regions and genes were previously related to the trait of interest, while the CRBN gene was to our knowledge presented in the context of trypanosomiasis tolerance for the first time.
Urinary minerals excretion among primary schoolchildren in Dubai—United Arab Emirates
Authors: Al Ghali Rola , El-Mallah Carla , Obeid Omar , El-Saleh Ola , Smail Linda , Haroun Dalia
Published: August 2021

Introduction

Urinary excretion of calcium (Ca), magnesium (Mg), phosphorus (P), iodine and fluoride is used to assess their statuses and/or the existence of metabolic abnormalities. In the United Arab Emirates (UAE), the urinary concentration of these minerals among children have not been documented.

Materials and methods

A cross-sectional study, including 593 subjects (232 boys and 361 girls), was conducted among healthy 6 to 11-year-old Emirati children living in Dubai. Non-fasting morning urine samples and anthropometrical measurements were collected and analyzed. Results were expressed as per mg of creatinine (Cr).

Results

On average, estimated Cr excretion was 17.88±3.12 mg/kg/d. Mean urinary Ca/Cr, Mg/Cr and P/Cr excretions were 0.08±0.07 mg/mg, 0.09±0.04 mg/mg, and 0.57±0.26 mg/mg respectively. Urinary excretion of Ca, Mg and P were found to decrease as age increased. Urinary excretion and predicted intake of fluoride were lower than 0.05 mg/kg body weight per day. Surprisingly, more than 50% of the children were found to have urinary iodine excretion level above adequate.

Conclusion

The Emirati schoolchildren had comparable levels of urinary Ca, Mg and P excretion to other countries. The 95% percentile allows the use of the current data as a reference value for the detection of mineral abnormalities. Fluoride excretion implies that Emirati children are at low risk of fluorosis. The level of urinary iodine excretion is slightly higher than recommended and requires close monitoring of the process of salt iodization to avoid the harmful impact of iodine overconsumption.
A 50 Hz magnetic field affects hemodynamics, ECG and vascular endothelial function in healthy adults: A pilot randomized controlled trial
Authors: Okano Hideyuki , Fujimura Akikatsu , Kondo Tsukasa , Laakso Ilkka , Ishiwatari Hiromi , Watanuki Keiichi
Published: August 2021

Application of exposure to 50/60 Hz magnetic fields (MFs) has been conducted in the treatment of muscle pain and fatigue mainly in Japan. However, whether MFs could increase blood flow leading to muscle fatigue recovery has not been sufficiently tested. We investigated the acute effects of a 50 Hz sinusoidal MF at Bmax 180 mT on hemodynamics, electrocardiogram, and vascular endothelial function in healthy young men. Three types of regional exposures to a 50 Hz MF, i.e., forearm, upper arm, or neck exposure to MF were performed. Participants who received three types of real MF exposures had significantly increased ulnar arterial blood flow velocity compared to the sham exposures. Furthermore, after muscle loading exercise, MF exposure recovered hemoglobin oxygenation index values faster and higher than sham exposure from the loading condition. Moreover, participants who received real MF exposure in the neck region had significantly increased parasympathetic high-frequency activity relative to the sham exposure. The MF exposure in the upper arm region significantly increased the brachial artery flow-mediated dilation compared to the sham exposure. Computer simulations of induced in situ electric fields indicated that the order-of-magnitude estimates of the peak values were 100–500 mV/m, depending on the exposure conditions. This study provides the first evidence that a 50 Hz MF can activate parasympathetic activity and thereby lead to increase vasodilation and blood flow via a nitric oxide-dependent mechanism.

Trial registration: UMIN Clinical Trial Registry (CTR) UMIN000038834. The authors confirm that all ongoing and related trials for this drug/intervention are registered.
ELBW and ELGAN outcomes in developing nations–Systematic review and meta-analysis
Authors: Ramaswamy Viraraghavan Vadakkencherry , Abiramalatha Thangaraj , Bandyopadhyay Tapas , Shaik Nasreen Banu , Bandiya Prathik , Nanda Debasish , Pullattayil S. Abdul Kareem , Murki Srinivas , Roehr Charles Christoph
Published: August 2021

Context

Morbidity and mortality amongst extremely low birth weight (ELBW) and extremely low gestational age neonates (ELGANs) in developing nations has not been well studied.

Objectives

Evaluate survival until discharge, short- and long-term morbidities of ELBW and ELGANs in LMICs.

Data sources

CENTRAL, EMBASE, MEDLINE and Web of Science.

Study selection

Prospective and retrospective observational studies were included.

Data extraction and synthesis

Four authors extracted data independently. Random-effects meta-analysis of proportions was used to synthesize data, modified QUIPS scale to evaluate quality of studies and GRADE approach to ascertain the certainty of evidence (CoE).

Results

192 studies enrolling 22,278 ELBW and 18,338 ELGANs were included. Survival was 34% (95% CI: 31% - 37%) (CoE–low) for ELBW and 39% (34% - 44%) (CoE—moderate) for ELGANs. For ELBW neonates, the survival for low-income (LI), lower middle-income (LMI) and upper middle income (UMI) countries was 18% (11% - 28%), 28% (21% - 35%) and 39% (36% - 42%), respectively. For ELGANs, it was 13% (8% - 20%) for LI, 28% (21% - 36%) for LMI and 48% (42% - 53%) for UMI countries. There was no difference in survival between two epochs: 2000–2009 and 2010–2020. Except for necrotising enterocolitis [ELBW and ELGANs—8% (7% - 10%)] and periventricular leukomalacia [ELBW—7% (4% - 11%); ELGANs—6% (5%-7%)], rates of all other morbidities were higher compared to developed nations. Rates of neurodevelopmental impairment was 17% (7% - 34%) in ELBW neonates and 29% (23% - 37%) in ELGANs.

Limitations

CoE was very low to low for all secondary outcomes.

Conclusions

Mortality and morbidity amongst ELBW and ELGANs is still a significant burden in LMICs. CoE was very low to low for all the secondary outcomes, emphasizing the need for high quality prospective cohort studies.

Trial registration

PROSPERO (CRD42020222873).
6-month mortality and readmissions of hospitalized COVID-19 patients: A nationwide cohort study of 8,679 patients in Germany
Authors: Günster Christian , Busse Reinhard , Spoden Melissa , Rombey Tanja , Schillinger Gerhard , Hoffmann Wolfgang , Weber-Carstens Steffen , Schuppert Andreas , Karagiannidis Christian
Published: August 2021

Background

COVID-19 frequently necessitates in-patient treatment and in-patient mortality is high. Less is known about the long-term outcomes in terms of mortality and readmissions following in-patient treatment.

Aim

The aim of this paper is to provide a detailed account of hospitalized COVID-19 patients up to 180 days after their initial hospital admission.

Methods

An observational study with claims data from the German Local Health Care Funds of adult patients hospitalized in Germany between February 1 and April 30, 2020, with PCR-confirmed COVID-19 and a related principal diagnosis, for whom 6-month all-cause mortality and readmission rates for 180 days after admission or until death were available. A multivariable logistic regression model identified independent risk factors for 180-day all-cause mortality in this cohort.

Results

Of the 8,679 patients with a median age of 72 years, 2,161 (24.9%) died during the index hospitalization. The 30-day all-cause mortality rate was 23.9% (2,073/8,679), the 90-day rate was 27.9% (2,425/8,679), and the 180-day rate, 29.6% (2,566/8,679). The latter was 52.3% (1,472/2,817) for patients aged ≥80 years 23.6% (1,621/6,865) if not ventilated during index hospitalization, but 53.0% in case of those ventilated invasively (853/1,608). Risk factors for the 180-day all-cause mortality included coagulopathy, BMI ≥ 40, and age, while the female sex was a protective factor beyond a fewer prevalence of comorbidities. Of the 6,235 patients discharged alive, 1,668 were readmitted a total of 2,551 times within 180 days, resulting in an overall readmission rate of 26.8%.

Conclusions

The 180-day follow-up data of hospitalized COVID-19 patients in a nationwide cohort representing almost one-third of the German population show significant long-term, all-cause mortality and readmission rates, especially among patients with coagulopathy, whereas women have a profoundly better and long-lasting clinical outcome compared to men.
Prevalence of unculturable bacteria in the periapical abscess: A systematic review and meta-analysis
Authors: Altaie Alaa Muayad , Saddik Basema , Alsaegh Mohammed Amjed , Soliman Sameh S. M. , Hamoudi Rifat , Samaranayake Lakshman P.
Published: August 2021

Objective

To assess the prevalence of unculturable bacteria in periapical abscess, radicular cyst, and periapical granuloma.

Methods

PubMed, Scopus, Science Direct, and Ovid databases were systematically searched from January 1990 to May 2020. All the included studies were cross-sectional design. The risk of bias was assessed using Joanna Briggs Institute check-list. Heterogeneity was described using meta-regression and mixed-effects model for lesion, country, and sequence technique moderators. Funnel plot and unweighted Egger’s regression test were used to estimate the publication bias. Microbiome data on diversity, abundance, and frequency of unculturable bacteria in the periapical lesions were reviewed, analysed, and the principal component analysis (PCA) was performed.

Results

A total of 13 studies out of 14,780, were selected for the final analysis. These studies focused on the prevalence of unculturable bacteria in periapical abscesses and related lesions. Approximately 13% (95% CI: 7–23%) of the cumulative number of bacteria derived from periapical abscesses was unculturable. Country moderator significantly (P = 0.05) affects the diversity summary proportion. While the pooled frequency of unculturable bacteria was 8%; 95% CI: 5, 14%, the estimate of the pooled abundance of unculturable bacteria was 5%; 95% CI: 2, 12% with a significant (P = 0.05) country moderator that affects the abundance summary proportion. Of the 62 unculturable bacteria, 35 were subjected to PCA and Peptostreptococcus sp. oral clone CK035 was the most abundant species in periapical abscesses. Hybridization techniques were found to be the most reliable molecular methods in detecting the abundance and frequency of unculturable bacteria.

Conclusion

The significant prevalence of unculturable bacteria in the periapical abscess, suggests that they are likely to play, a yet unknown, critical role in the pathogenesis and progression of the disease. Further research remains to be done to confirm their specific contributions in the virulence and disease progression.
Clinical relevance for circulating cold-inducible RNA-binding protein (CIRP) in patients with adult-onset Still’s disease
Authors: Fujita Yuya , Yago Toru , Asano Tomoyuki , Matsumoto Haruki , Matsuoka Naoki , Temmoku Jumpei , Sato Shuzo , Yashiro-Furuya Makiko , Suzuki Eiji , Watanabe Hiroshi , Kawakami Atsushi , Migita Kiyoshi
Published: August 2021

Background

Adult-onset Still’s disease (AOSD) is a systemic autoinflammatory disease in which danger-associated molecular patterns (DAMPs)-mediated inflammasome activation seems to be involved in the disease pathogenesis. Cold-inducible RNA-binding protein (CIRP) belongs to a family of cold-shock proteins that respond to cellular stress and has been identified as a DAMP that triggers the inflammatory response. The aim of this study is to investigate the clinical significance of serum CIRP levels in AOSD.

Methods

Serum samples were obtained from 44 patients with active AOSD or 50 patients with rheumatoid arthritis (RA), 20 patients with systemic lupus erythematosus (SLE), and 15 healthy control patients (HCs). Serum levels of CIRP and IL-18 were determined using enzyme-linked immunosorbent assay. Results were compared among AOSD patients, RA patients, SLE patients and HCs. Results were also analyzed according to the clinical features of AOSD.

Results

Serum CIRP levels were significantly higher in AOSD patients compared with RA patients (median: 9.6 ng/mL, IQR [5.7–14.4] versus 3.2 ng/mL, IQR [1.9–3.8]; p < 0.001) and with HCs (2.8 ng/mL, [IQR; 1.4–4.9], p < 0.001). There was a significant positive correlation between serum CIRP levels and AOSD disease activity score (Pouchot’s score r = 0.45, p = 0.003) as well as between AOSD-specific biomarkers ferritin and IL-18. However, there was no significant difference in the serum CIRP levels among AOSD patients with three different disease phenotypes.

Conclusions

These results suggest that CIRP may play a significant role in the pathophysiology of AOSD and could be a potential biomarker for monitoring the disease activity of AOSD.
Examining the trade-offs between human fertility and longevity over three centuries using crowdsourced genealogy data
Authors: Hsu Chen-Hao , Posegga Oliver , Fischbach Kai , Engelhardt Henriette
Published: August 2021

The evolution theory of ageing predicts that reproduction comes with long-term costs of survival. However, empirical studies in human species report mixed findings of the relationship between fertility and longevity, which varies by populations, time periods, and individual characteristics. One explanation underscores that changes in survival conditions over historical periods can moderate the negative effect of human fertility on longevity. This study investigates the fertility-longevity relationship in Europe during a period of rapid modernisation (seventeenth to twentieth centuries) and emphasises the dynamics across generations. Using a crowdsourced genealogy dataset from the FamiLinx project, our sample consists of 81,924 women and 103,642 men born between 1601 and 1910 across 16 European countries. Results from multilevel analyses show that higher fertility has a significantly negative effect on longevity. For both women and men, the negative effects are stronger among the older cohorts and have reduced over time. Moreover, we find similar trends in the dynamic associations between fertility and longevity across four geographical regions in Europe. Findings and limitations of this study call for further investigations into the historical dynamics of multiple mechanisms behind the human evolution of ageing.
Does age-dynamic movement accelerate facial age impression? Perception of age from facial movement: Studies of Japanese women
Authors: Kurosumi Motonori , Mizukoshi Koji , Hongo Maya , Kamachi Miyuki G.
Published: August 2021

We form impressions of others by observing their constant and dynamically-shifting facial expressions during conversation and other daily life activities. However, conventional aging research has mainly considered the changing characteristics of the skin, such as wrinkles and age-spots, within very limited states of static faces. In order to elucidate the range of aging impressions that we make in daily life, it is necessary to consider the effects of facial movement. This study investigated the effects of facial movement on age impressions. An age perception test using Japanese women as face models was employed to verify the effects of the models’ age-dependent facial movements on age impression in 112 participants (all women, aged 20–49 years) as observers. Further, the observers’ gaze was analyzed to identify the facial areas of interests during age perception. The results showed that cheek movement affects age impressions, and that the impressions increase depending on the model’s age. These findings will facilitate the development of new means of provoking a more youthful impression by approaching anti-aging from a different viewpoint of facial movement.
Using the first nationwide survey on non-communicable disease risk factors and different definitions to evaluate the prevalence of metabolic syndrome in Burkina Faso
Authors: Cissé Kadari , Samadoulougou Délwendé René Séverin , Bognini Joel Dofinissery , Kangoye Tiga David , Kirakoya-Samadoulougou Fati
Published: August 2021

Background

The burden of cardiovascular diseases is rising in the developing world including Sub-Saharan Africa. The rapid rise of cardiovascular disease burden is in part due to undetected and uncontrolled cardiovascular risk factors. The clustering of metabolic syndrome (MetS) components is associated with a high risk of cardiovascular diseases. This complex biochemical disorder is still poorly studied in western Africa. In this study, we aimed to determine the prevalence of metabolic syndrome and its determinants among the adult population in Burkina Faso.

Methods

We performed a secondary analysis of the data from the first national survey on non-communicable diseases risk factors using the World Health Organization (WHO) Stepwise approach. We included 4019 study participants aged 25 to 64 years. The metabolic syndrome prevalence was estimated using six different definitions.

Results

The mean age was 38.6±11.1 years. Women represented 52.4% and three quarters (75%) lived in rural areas. The overall prevalence of metabolic syndrome according to the different definitions was 1.6% (95%CI:1.1–2.2) for the American College of Endocrinology, 1.8% (95%CI: 1.3–2.4) for the WHO, 4.3% (95%CI:3.5–5.2) for the National Cholesterol Education Program Adult Treatment Panel III, 6.2% (95%CI: 5.1–7.6) for the AAH/NHBI, 9.6%(95%CI: 8.1–11.3) for the International Diabetes Federation and 10.9% (95%: 9.2–12.7) for the Joint Interim Statement. The metabolic syndrome components with the highest prevalence were low High density lipoprotein (63.3%), abdominal obesity (22.3%) and hypertension (20.6%). People living in urban areas and those with older age have higher prevalence of metabolic syndrome regardless of the definition used.

Conclusion

Our findings suggest various levels of prevalence of MetS according to the definition used. Identifying the most appropriate criteria for MetS among the adult population is important to early detect and treat this syndrome and its components at the primary health care level to control the rising burden of cardiovascular diseases in the context of ongoing epidemiological transition in the country.
Primary care-based screening and management of depression amongst heavy drinking patients: Interim secondary outcomes of a three-country quasi-experimental study in Latin America
Authors: O’Donnell Amy , Schulte Bernd , Manthey Jakob , Schmidt Christiane Sybille , Piazza Marina , Chavez Ines Bustamante , Natera Guillermina , Aguilar Natalia Bautista , Hernández Graciela Yazmín Sánchez , Mejía-Trujillo Juliana , Pérez-Gómez Augusto , Gual Antoni , de Vries Hein , Solovei Adriana , Kokole Dasa , Kaner Eileen , Kilian Carolin , Rehm Jurgen , Anderson Peter , Jané-Llopis Eva
Published: August 2021

Introduction

Implementation of evidence-based care for heavy drinking and depression remains low in global health systems. We tested the impact of providing community support, training, and clinical packages of varied intensity on depression screening and management for heavy drinking patients in Latin American primary healthcare.

Materials and methods

Quasi-experimental study involving 58 primary healthcare units in Colombia, Mexico and Peru randomized to receive: (1) usual care (control); (2) training using a brief clinical package; (3) community support plus training using a brief clinical package; (4) community support plus training using a standard clinical package. Outcomes were proportion of: (1) heavy drinking patients screened for depression; (2) screen-positive patients receiving appropriate support; (3) all consulting patients screened for depression, irrespective of drinking status.

Results

550/615 identified heavy drinkers were screened for depression (89.4%). 147/230 patients screening positive for depression received appropriate support (64%). Amongst identified heavy drinkers, adjusting for country, sex, age and provider profession, provision of community support and training had no impact on depression activity rates. Intensity of clinical package also did not affect delivery rates, with comparable performance for brief and standard versions. However, amongst all consulting patients, training providers resulted in significantly higher rates of alcohol measurement and in turn higher depression screening rates; 2.7 times higher compared to those not trained.

Conclusions

Training using a brief clinical package increased depression screening rates in Latin American primary healthcare. It is not possible to determine the effectiveness of community support on depression activity rates due to the impact of COVID-19.