bluuebunny/crossref_metadata_2025 huggingface dataset:
Analysis of Educational Migration Decision in Indonesia
Authors: Mustika Made Dwi Setyadhi , Remi Sutyastie Soemitro , Fahmi Mohamad , Setiawan Maman
Published: November 2022

Generally, migration is the movement of a person from one to another region. Besides that, several factors may cause migration such as uneven economic and educational development. This study aims to discover the factors of educational migration, particularly in Indonesia. This study carried out Indonesian Family Life Surveys (IFLS) microdata; however, the IFLS divides into IFLS wave 3 (2000), IFLS wave 4 (2007), and IFLS wave 5 (2014). Hence, the total sample of the study is 1.878 people. In addition, The Logistics Regression Model or Logit Model analysis technique is used in this study. This study found that educational migration factors are influenced by personal consumption expenditure (PCE), geographical conditions, gender, high school education, university education, age, religion, cognitive ability, father's educational background (High School and university), mother's educational background (High School and university), and the province of origin. To sum up, the future welfare of a person is influenced by educational migration.

 

 

Received: 8 August 2022 / Accepted: 10 October 2022 / Published: 5 November 2022
Investigating the Influence of Awards on Teacher’s Motivation Towards Performance: A Study of Selected Primary School in Delta State Nigeria
Authors: Mbukanma Ifeanyi
Published: November 2022

Orientation: Awards are mostly given on the basis of recognitions without ascertaining it influence on the current state of awardee and their future performance. Research purpose: The main purpose of this study was to ascertain the influence of awards on teacher’s motivation towards optimal performance. Motivation: This study was motivated by the existing gap in the previous literature on how awards actually motivate teachers and other better ways to motivate teachers towards optimal performance. Research method: A qualitative study and a participatory action research design were employed, where primary schools in Delta State, Nigeria, were sampled. Data was collected using written interview techniques, and the subjective thoughts of the participants were vital in this study to ascertain the influence of awards on teacher’s motivation towards optimal performance. Analysis: To analyse the participant’s responses, a thematic technique for data analysis was employed using ATLAS.ti version 9, which helped the researchers categorise selected themes from the responses collected from participants. Findings: The study result revealed that although awards contribute significantly to teachers’ motivation, teachers’ motivation and optimal performance could also be cushioned by professional development, encouragement by superiors, learners’ performance, learners’ participation, acknowledgement of hard work, competitive salary, and fringe benefits. Managerial implications and contribution: Thus, since the study was carried out to provide a practical academic-based solution to a real-life scenario, the contribution of this study (Integrated Model for Primary School Teacher’s Awards and Motivation) was recommended to the concerned stakeholders in the primary education department in Delta state, Nigeria.

 

Received: 12 August 2022 / Accepted: 25 October 2022 / Published: 5 November 2022
Accelerated Learning and Exploring the Future of Professional Development
Authors: Moustaffa Naglaa
Published: November 2022

This study aimed to reveal faculty members' impressions of the effectiveness of a sustainable professional development model derived from accelerated learning principles. The semi-experimental approach and its approved descriptive and analytical approaches collect data through a questionnaire directed at a study sample of 55 members from the Faculty of Education at Prince Sattam Bin Abdulaziz University in Saudi Arabia. The study used descriptive statistics to describe frequencies, ratios, means, and standard deviations, and Cronbach's alpha coefficient, Pearson correlation coefficient, independent-samples t-test, and one-way analysis of variance (ANOVA). The study results indicate a consensus about the model's effectiveness as applied at the institutional and individual levels, and there are no statistically significant differences in impressions due to gender, age, work experience, or academic degree. The study reached the conclusion that examining the applied model for professional development based on accelerated learning principles showed that investing in information and communication technology was effective within the study's limits. However, to evaluate the effectiveness of the application, the study recommended that the researchers should evaluate all dimensions of the professional development model in different contexts and enrich the educational research library with more professional development studies and models for applying the accelerated learning principles.

 

Received: 2 August 2022 / Accepted: 7 October 2022 / Published: 5 November 2022
Impact of Urbanization and Economic Growth in Albania
Authors: Lubonja Otjela , Shehu Elona
Published: November 2022

One of the goals of the study is to analyze how urbanization and economic growth are related, identify the elements that influence a nation's urbanization, and add to the body of theoretical and empirical research on urbanization. Over the past 32 years, Tirana has undergone a number of swift and challenging transformations in the economic, social, and political arenas. The sudden transformation of the natural and populated terrain serves as a stark example of an unforeseen and novel phenomenon for Albanian society. Various researchers have confirmed this statement but no previous study has used socio-economic data to study this relationship in the city of Tirana, as a city with a promising economic development. Urbanization is linked to problems including insufficient housing, poor waste management, and insufficient infrastructure, all of which are very difficult to eradicate and control. These problems are still a battle in developed nations. Tirana and other developing cities have a much higher rate of urbanization than did the wealthy nations of today when they were first developing (Dutt and Parai, 1994). Through an examination of the causal relationships between variables and their effects on the economy, this study looks at other aspects of population migration. This study is analytical and exploratory. The research findings can support attempts to discover acceptable solutions to urban problems, particularly in Tirana and beyond, by providing an up-to-date understanding of the elements that project urbanization. The study is based on contemporary urbanization theory, which is particularly relevant in the instance of Tirana. The following ways that this study will increase knowledge are as follows: it will pinpoint socioeconomic factors that attempt to forecast Tirana's urbanization; it will develop a method to measure urbanization and economic growth through a standardized measure. The basic methods according to which such an evaluative analysis will be made are related to the correlation between the variables as well as the regression analysis. The data have been obtained from INSTAT and the World Bank for the period 1998-2019.

 

Received: 5 September 2022 / Accepted: 28 October 2022 / Published: 5 November 2022
Impact of Contaminated Water and Sediment on Fish Species of Peshawar Region, KPK, Pakistan
Authors: Khan Shamsher , Mateen Irfan
Published: November 2022

Rivers in the KPK Peshawar region of Pak are subjected to a variety of anthropogenic impacts, Agricultural operations, industry, and urban garbage are all examples. The purpose of The goal of this research was to find out the degree of water contamination by showing a testing that includes in situ bio-monitoring of water constituents on Geno-toxicity in local fish species. Three places in the region were tested for Geno-toxicity using the micronucleus test. At sample locations like the urban perimeter, farmland, and the fertiliser industry, it was interesting to observe if there were any differences between monitored species control and such Characidium fasciatum, Astyanax fasciatus, Astyanax altiparanae. Records showed that the species from sites one, Two, and Three had a considerably greater frequency of Micronucleus when compared to fish from site Four. The frequency of Micronucleus was substantially higher in and A. altiparanae and A. fasciatus erythrocytes at sites one and two. Micronucleus frequencies were higher at site three in C. fasciatum. Micronucleus induction in C. fasciatum was associated to Cr levels in water and sediment, whereas A. altiparanae and A. fasciatum were linked to zinc levels in water and sediment.

 

Received: 11 July 2022 / Accepted: 19 October 2022 / Published: 5 November 2022
Mirth as an Outlet of Repressed Feminine Yearnings in Carol Ann Duffy’s Poetry
Authors: Lum Mary Louisa
Published: November 2022

Subtlety is the only way wherein feminine rebellion can flourish and succeed in a male dominant society. This paper attempts to show that collaborative feminine effort leads to collective liberty and that Duffy (2002) pays homage to the feminist waves, especially the radical phase. The poet uses laughter as a metaphor to represent the feminist waves that were initially perceived as a joke but culminated in a force that ensured the emancipation of women. Although women’s desires are often repressed by the patriarchal machinery that seeks to oppress them, united action leads to liberation. Critics of Duffy have focused on her radical feminist and revisionist themes but this paper will also read the Laughter of Stafford Girls High (2002) as a homage to the various feminist waves that have contributed to women’s emancipation discourses globally. The paper draws from Wollstonecraft (1792) whose treatise A Vindication of the Rights of Woman exposed the triviality of women’s education and advocated for equality in learning. Although contemporary society enjoys parity in learning, several feminist critics like Howe (1977) and Hooks (1994) insist that there are still hidden messages within curriculums that tactfully ensure the marginal role of women. The absence of women in the canons gives the pervasive feeling that women have contributed nothing to human progress. The paper, therefore, aligns with the idea of feminine writing posited by Cixous (1976) as the only way through which women can write their unique history. From a reading of Duffy (2002) with the aforementioned theoretical leanings, it was realized that spaces of patriarchal brainwashing still abound despite the perceived progressive worldview. Equally, united action is important for the restitution of the rights of all women globally.

 

Received: 7 September 2022 / Accepted: 27 October 2022 / Published: 5 November 2022
Development, Validation and Standardization of Instrument to Measure Statistical Literacy among Universities Undergraduates in Cross River State
Authors: Ofem Usani Joseph , Ofem Isaac Ubi , Akpan Samuel , Ovat Sylvia Victor , Nworgwugwu C. E. , Otu Bernard , Atah Cletus Akpo
Published: November 2022

The study developed, validated, and standardised a statistical literacy test for university undergraduates in Cross River State, Nigeria. Five research questions were raised to guide this study. The study adopted an instrumentation design. A stratified sampling technique was used to select a total of 753 respondents from a population of 4705 students. Three instruments were used to collect data: the Statistical Literacy Test (SLT), the Statistical Reasoning Test (SRT), and the Statistical Anxiety Test (SAT). Descriptive statistics (means and simple percentages), correlational analysis, and the factor analytical technique were used to analyse the data collected. The result showed that only 25 items survived the last item assessment. The instrument was found to have strong construct validity as the items in the correlation matrix were all positively correlated. The statistical literacy scale showed convergence with statistical reasoning but diverged with statistical anxiety. The reliability of the instrument was found to be high as the coefficients of the scale were 0.81, 0.77, and 0.78 respectively, and it was adjudged reliable. Based on the findings of the study, it was concluded that the instrument was valid and reliable. Thus, it was recommended that students' skills in statistics should not just be on the computational aspect but on the utilisation of statistical knowledge. Statistics lecturers should emphasise statistical literacy to help students reason and think statistically, and the instrument should be widely distributed for use by researchers and psychometricians.

 

Received: 16 August 2022 / Accepted: 28 October 2022 / Published: 5 November 2022
LITERATURE REVIEW : PENGARUH KONSUMSI KOPI TERHADAP KADAR KOLESTEROL PADA PENGKONSUMSI KOPI
Authors: Krispila Anjelia , Kahanjak Donna Novina , Mutiasari Dian
Published: November 2022

Kolesterol adalah masalah kompleks bagi manusia pada saat kadar LDL lebih mendominasi menyebabkan penyakit jantung dan hipertensi akibat dari terjadinya aterosklerosis. Prevalensi di Amerika Serikat orang dewasa mengalami peningkatan kadar kolesterol >12%. Kalimantan Tengah data penderita kolesterol mencapai 54,2%. Kolesterol 2/3 diproduksi oleh hati sedangkan 1/3 nya diserap oleh sistem pencernaan dari makanan yang dikonsumsi. Kopi tidak mengandung kolesterol namun kopi mengandung kafestol dan kahwoel yang dapat larut dalam lemak sering disebut dengan diterpenes. Tujuan dari review tinjauan ini adalah mengetahui pengaruh kopi terhadap peningkatan kadar kolesterol. Penelitian ini menggunakan pendekatan systematic review dengan kata kunci kopi, kadar kolesterol, pengaruh konsumsi kopi, dengan metode PRISMA. Dari hasil pencarian, diperoleh 14 jurnal membahas tentang pengaruh konsumsi kopi terhadap kadar kolesterol dan berapa jumlah konsumsi kopi yang dapat mempengaruhi kadar kolesterol serta pengaruh konsumsi kopi terhadap kadar LDL. Sebanyak 12 jurnal (85,2%) menyatakan terdapat pengaruh yang signifikan sedangkan 2 jurnal (14,2%) menyatakan tidak ada pengaruh yang signifikan. Adapun dari hasil tinjauan literature, dapat disimpulkan bahwa terdapat pengaruh konsumsi kopi terhadap kadar kolesterol, jumlah konsumsi kopi yang dapat mempengaruhi kadar kolesterol dan pengaruh kopi terhadap kadar LDL
СФЕРА ВИЩОЇ ОСВІТИ ЯК СКЛАДОВА ПЛАНУ ВІДНОВЛЕННЯ УКРАЇНИ
Authors: Vitrenko Yurii , Vorona Viktoriia , Debych Mariia
Published: November 2022

У статті проаналізовано План відновлення України за напрямом «Освіта і наука», викладеним у розділі «Вища освіта», та основні показники сфери вищої освіти України у 2005-2020 рр. Аналіз показників сфери вищої освіти України у 2005-2020 рр. дає достатньо підстав для прогнозу, що динаміка чисельності студентів у 2022-2032 рр. продовжить знижуватися (у 1,5-2,0 рази до 2032 р.). Рекомендовано основні пріоритети у визначенні обсягів підготовки фахівців із вищою освітою у закладах вищої освіти України з оплатою виробництва і надання послуг у сфері вищої освіти за рахунок державного та регіональних бюджетів України, які мають бути враховані у формуванні Плану відновлення України за напрямом «Освіта і наука»: 1. Для Збройних сил України. 2. Для підготовки поліцейських, національних гвардійців, рятувальників, прикордонників. 3. Для підприємств військово-промислового комплексу України. 4. Для аграрно-промислового комплексу України. 5. Для сфери освіти України. 6. Для сфери охорони здоров’я України. Внесено пропозиції до Плану відновлення України за напрямом «Освіта і наука», розділу «Вища освіта» та до окремих нормативно-правових актів, які регулюють діяльність сфери вищої освіти.
The Rhetorical Structure In The Value Visibility In The Letters Of Imam Al-Baqir “B”
Authors: Badir Al-Amidi Dr. Huda Saeed
Published: November 2022

Among the clash of deviant ideas and dark forces’ struggle against the Muslim lands and in light of the unification of the enemy’s front and the division of the Muslim scattered between those who believe in the ongoing war, and between those who deny and those who reject the enemy’s front and those who clap for it, and between those who are weak and who do not have the right to benefit or harm themselves, there is the Imam of hearts who takes control of all, taking from the Qur’an a canal and a platform, making his weapon of knowledge and knowledge and his forehead of scholars and educated people, because war is a war of minds, a war of ignorance, obfuscation, distortion of facts and confusion of papers. The way to spoil their war plan was to educate young people, arm them with science, inject them with faith, and put them in schools that would help them sort the overlapping papers and decipher the events in an elaborate manner. From here, the researcher tries through this research. Standing on the most important factors that made the school of Imam Al-Baqir live immortal, and continue to be steadfast, against the dangers of currents that were invading the Islamic community at the time, and dragging them to our present time, and measuring the extent of their convergence with the currents of today. For salvation, there is a way to save the Shiites of Imam al-Baqir, may God’s prayers be upon him, who live in the same conditions. This will be through the presentation of the letters of Imam Al-Baqir and his writings to his Shiites and his companions, which were collected by Sheikh Ahmed Al-Mayanji in his book (The Books of the Imams) in the third part of the book, where the book contained the writings of all the Imams and their writings that came in their own handwriting or dictated to one of their companions, and the book is verified by a professor The Seminary in the Holy Qom; Mujtaba Al-Farji. The book was revised several times under the supervision of the author's offspring.
THE ROLE OF CREATIVE LEADERSHIP IN ACHIEVING CREATIVE PERFORMANCE
Authors: Jawad Hasan Ghafil
Published: November 2022

Studying the role of leadership and its sciences in scientific research. The company has developed its time, and linked it between international companies represented in the research to the relationship between creative leadership and performance, testing and measuring it, creative leadership and its role in achieving performance and reaching only good results. And the reason for the success of the organizations and their announcement is the interest in the workers in the name of the organization and their containment in a way that makes them feel that they are from that organization. As for the most important recommendations, the leaders of organizations should choose a distinctive pattern of positive leaders, represented by creative leadership and lofty values, to gain the energies and experiences of the workers and make them more creative in their performance in the organization. To outstanding creative performance in addition to what you provide and then working hard and working hours for employees will lead to the employee's well-being and then his loyalty and adherence to it
THE METHODS OF PERSUASION USED IN EMARKETING ADVERTISEMENTS AND THEIR REFLECTION ON THE PURCHASING BEHAVIOUR OF IRAQI YOUTH: A FIELD STUDY
Authors: Al-Saedi Abdulhussein Juhi Mozan , Al-Moussawi Dr. Kareem Meshat Zalaf
Published: November 2022

Scientists consider that the appeal to the mind is the mental criterion for persuasion, which is the strongest and most influential, followed by the appeal to the heart, which represents the symbol of emotion, in the persuasive process. The Iraqi (field study) with several sub-questions, to achieve several research goals, based on the theory oriented to the research, which is (the theory of media persuasion), which we see as the most appropriate theory for the problem of this research, its questions and objectives, based on the intellectual heritage represented by the methods of persuasion in electronic advertisements and their reflection on young consumers. The method used is the survey method, as it is one of the most appropriate approaches to media studies, and is consistent with the topic, problem and objectives of the research based on the analytical descriptive approach, as well as the questionnaire tool for all data and information related to the field study of the research sample represented by the Iraqi youth in the Iraqi governorates (Baghdad, Nineveh and Basra) and the adult Their number is (668) researched. The study reached several results, including the following: Determining goals was one of the most important methods of persuasion used in electronic advertisements in Iraqi commercial sites. The polarization model occupied the forefront of the persuasion models used in electronic advertisements, because this model plays a positive role in persuading young consumers and urging them to make a purchase decision. Among the most important principles of persuasive design in electronic advertising is simplicity and clarity The most important recommendations that came out of this study are as follows: Highlighting the characteristics and advantages of the product in terms of quality and efficiency, as well as focusing on the commercial relationship, facility, price and warranty in order to urge young people to make a purchase decision. The electronic advertisements in the Iraqi commercial sites focused on advertising communication, but they neglected electronic sales, and the delivery service, which is among the pillars of delivery. The necessity of paying attention to persuasive methods in electronic
THE INFLUENCE OF THE MEDIA ON THE DEVELOPMENT OF DOMESTIC TOURISM IN THE ANCIENT CITY OF UR: AN EXPLORATORY STUDY OF A SAMPLE OPINION OF TOURISTS
Authors: Qasem Nisreen Ghali
Published: November 2022

The increasing role of the media in supporting the national development plans of countries is not hidden, especially in the field of tourism, which is one of the most important economic resources that helps in maximizing revenues. The tremendous technology in the field of communication techniques, and this research came to shed light on the role of media in the sustainable tourism development process through three sections. Sustainable tourism development, and the research reached a set of conclusions, the most important of which are: 1 - The tourism media has a great ability to change people's cultures, and this influence is coupled with the tourism services provided on the ground. 2- The principle of tourism development and achieving its sustainability is a basic requirement in the field of tourism because of its peculiarities that distinguish it from other sectors
Economic Aspects of Organic Farming
Authors: Chaudhary Poornima
Published: November 2022

This article examines the economic and environmental factors unique to tiny, landless, and marginal farmers, and then suggests ways in which such farmers might increase their income via the practice of organic farming. The majority of farmers are economically marginalized, making it unrealistic to expect them to invest in large quantities of fertilizers and chemicals, thus they must turn to organic practises instead. And for the many individuals who own just a few acres of farmland, organic farming is a great option. The Fair-Trade Alliance of Kerala (FTAK) is a group of local farmers with the goal of expanding their access to the international fair-trade market. In comparison to the free market, the method results in lower prices for goods, which is good news for fair trade exporters. By analysing FTAK, this study highlights its positive effects on income and highlights the importance of the growing organic food industry, premium pricing, and incentives for farmers. According to the findings, organic farming is a more effective way to increase Indian farmers' income by 20-50%, and that fair trade farmers received higher prices (20-50%) for their commodities when selling them directly to international buyers.
EFFECT OF EMPOWERING HR ON BUILDING THE REPUTATION OF THE EDUCATIONAL ORGANIZATION: AN EXPLORATORY STUDY OF A SAMPLE OF EMPLOYEES OF THE COLLEGE OF ADMINISTRATION AND ECONOMICS AT ALMUSTANSIRIYA UNIVERSITY
Authors: Haraj Ail Hassan
Published: November 2022

The aim of the research is to determine the level of influence to empower HR in building the reputation of the educational organization, as workers are one of the most important resources that organizations possess compared to other resources that they enjoy. The College of Administration and Economics at Al-Mustansiriya University in Baghdad Governorate as a field for application, and the research sample was chosen in a simple random way, consisting of (124) individuals from HR. Depending on a number of ready-made measures after being subjected to tests of validity and reliability, the ready-made statistical program Spss was used to enter and analyze the research data. Among the statistical tools that were used in the analysis are: frequencies, percentages, weighted arithmetic mean, standard deviation, relative importance, correlation coefficient to measure Internal validity, Cronbach's alpha coefficient, simple and multiple linear regression Statistical on a number of results, Jamie confirmed the existence of a significant effect with statistical significance at the level (0.05) to enable workers to build the reputation of the educational organization, and accordingly the researcher recommended the need to increase the interest of the educational organization in empowering its employees in a way that is reflected in improving their performance and then the performance of the college as a whole.
THE ROLE OF ENTREPRENEURIAL ORIENTATE AND INFORMATION TECHNOLOGY IN ANALYTICAL RESEARCH IN THE ELECTRICAL AND ELECTRONIC INDUSTRIES
Authors: Khalil Asra Abdu Al Salam
Published: November 2022

The research aims to present the theoretical implications of both the strategic leadership and the institutional performance of the employees, as well as to identify the extent of the surveyed private colleges for strategic leaders and the levels of institutional performance of the employees in the surveyed colleges. For the research sample and the second axis, the questionnaire questions for the variables, and each of (Al-Mamoun University College, Al-Isra University College, Al-Turath University College, Al-Rafidain University College, Baghdad College of Economic Sciences, AlNisour University College) was chosen as a research community, and (160) forms were distributed and (150) were retrieved. A valid form for statistical analysis, and the research problem was to identify the role played by the strategic leadership in private colleges, the research community, a role in developing institutional performance in them, and in order to process the data collected by the researcher by the research sample, the statistical analysis program (AMOS V25) was relied on. ) and the program SPSS V25)) and a number of descriptive and analytical statistical methods represented by (percentage, arithmetic mean, standard deviation , coefficient of variation, simple correlation coefficient, simple linear regression analysis and confirmatory factor analysis), the research reached a number of conclusions, the most important of which is that the leaders in the researched colleges have a strategic direction and the ability to closely examine their internal environment, their strengths and weaknesses, and their external environment related to opportunities And threats by participating in the development of that direction to achieve its goals, and accordingly the researcher recommends the leadership of the researched colleges to qualify individuals and work to direct them towards paying attention to the strategic direction of their ideas to enable them to make the right decision in the situations they are exposed to when carrying out the tasks assigned to them.
Awareness Level of Workers in the Management of Medical Waste Generated from Hospitals in Bayelsa State
Authors: Ikpokpo E. , Ogbonna D. N. , Abere S. A. , Gobo A. E. , Bibye A. A. S.
Published: November 2022

There is an increase awareness of the public on the dangers associated with the improper disposal of medical waste in this country predominantly waste contaminated with communicable disease agent. Given the general lack of knowledge and effective practice of medical waste documentation about the composition of bio-hazardous waste and the large amount generated, it becomes so difficult to evaluate strategies for waste reduction. The present work presents awareness level of workers in the management of medical waste generated from hospitals in Bayelsa State. The study aimed at assessing the awareness levels and knowledge on the documentation of medical waste among healthcare workers in the various healthcare institutions. The waste generated were weighed kg at source (various unit) for days, week monthly for a period of 3nonths at various unit in the health facilities. Other relevant data were obtained via well-structured questionnaire, and considering the Cochran formula, a sample size of 300 was obtained. Data were further analysed using simple percentage, frequency and charts. Result indicated that there was very poor awareness level of workers in the management of medical waste generated from hospitals in Bayelsa State, and that the level of knowledge on waste documentation as a practice of medical waste management was poor and supercilious in a twenty first century medical waste management process. Thus, it was recommended that the concern government agency should carry out sensitization, awareness, training and retraining of medical workers at the various healthcare institutions on effective medical waste management. Staff of the various hospitals should be properly trained on significance of documentation of waste generated in the various unit on the health facility and there should be provision of all medical waste management infrastructures to cushion the likely impact of these wastes on the on the workers and the general environment.
SIMULTANEOUS GENOMIC KNOCKOUT OF THE PRO-APOPTOTIC BAX, BAK1 GENES AND OVEREXPRESSION OF THE ANTI-APOPTOTIC PROTEIN BCL-2 AND THE AUTOPHAGY INDUCER BECLIN-1 GREATLY INCREASE THE INTEGREL CELL DENSITY AND LIFESPAN IN CHO S SUSPENSION CELL CULTURE
Authors: Gayamova E.A. , Dayanova L.K. , Kovnir S.V. , Vorobyov I.I.
Published: November 2022

Using two genome editing operations, an CHO A4 cell line was obtained with homozygous knockouts of the bak1, bax, dhfr, glul genes and insertion of the additional copies of bcl-2, beclin-1 genes. This cell line is capable of long- term growth without replacing the culture medium and has an increased integral cell density by 5 times in this mode of cultivation.
OBTAINING AND ASSESSING IMMUNOGENICITY OF A FUSION PROTEIN WHICH CONSISTS OF A RECEPTOR-BINDING DOMAIN (RBD) OF CORONAVIRUS SARS-COV-2 SPIKE PROTEIN AND A MONOMERIC NON- GLYCOSYLATED FC-FRAGMENT OF HUMAN IGG1
Authors: Ryazanova A.Yu. , Orlova N.A. , Sinegubova M.V. , Dayanova L.K. , Kovnir S.V. , Korobova S.V. , Ledov V.A. , Kovalchuk A.L. , Aparin P.G. , Vorobiev I.I.
Published: November 2022

Variants of genetic constructions are discussed which encode a receptor-binding domain (RBD) of coronavirus SARS-CoV-2 spike protein fused with a monomeric Fc-fragment of human immunoglobulins. A cell line producing RBD-Fc is established. High titers of anti-RBD antibodies and virus-neutralizing antibodies are obtained upon mice immunization with the purified RBD-Fc.
RECOGNITION OF SODIUM-DEPENDENT PHOSPHATE TRANSPORTER NAPI2B BY MONOCLONAL ANTIBODIES IN BACTERIAL AND EUKARYOTIC CELLS
Authors: Bulatova L.F. , Kiyamova R.G.
Published: November 2022

The membrane sodium-dependent phosphate transporter NaPi2b is attractive target for monoclonal antibody therapy. Recognition of the large extracellular domain (ECD) of NaPi2b by monoclonal antibodies L2(20/3) in Western blot analysis depends on the addition of DTT to lysates of eukaryotic cells, but not bacterial cells.
IN VITRO CELL-BASED ASSAYS FOR SCREENING AND COMPARISON OF TNF-Α AND IL-17A INHIBITORS AND THEIR BIOSIMILARS
Authors: Pugachev E.I. , Volova L.T.
Published: November 2022

Cell-based bioassays have been developed to assess the biological activity of TNF-α and IL-17A inhibitors based on primary human cell cultures obtained from tissues of juvenile donors. The Fibro-bioassay is based on the analysis of IL-17A dose-dependent production of inflammatory cytokines synthesized by human foreskin fibroblasts (HFF). Using this bioassay, it has been shown that Netakimab (Biocad, RF) is a highly effective inhibitor of IL-17A. Using the Chondro test system the biological activity of TNF-α inhibitors was compared: the original molecule Remicade (MSD, Ireland) with its biosimilar Infliximab (Biocad, Russia).
SELECTIVE DETERMINATION OF CEPHALOSPORINS BY ENZYME-LINKED RECEPTOR ASSAY
Authors: Sviridov O.V.
Published: November 2022

A new approach has been proposed for the determination of beta-lactam antibiotics of the cephalosporin group in the presence of penicillins by enzyme-linked receptor assay (ELRA) using recombinant beta-lactam receptor protein BlaR-CTD immobilized on the solid phase. The cross-reaction is suppressed by adding high-affinity anti-penicillin polyclonal antibodies to the liquid phase of the system.
ISOLATION OF MICROMYCETES FROM FAT-CONTAINING WASTE OF THE FOOD INDUSTRY AND SCREENING OF LIPASE PRODUCERS
Authors: Glukhova L.B. , Ivasenko D.A. , Frank Yu.A.
Published: November 2022

Fungal isolates capable of fat hydrolysis have been isolated from the solid fat waste of food processing plants and studied. A laboratory test on the bioconversion of fat mass in the peat-fat mixture by micromycetes was carried out.
SCREENING FOR MICROORGANISMS CAPABLE OF EFFECTIVELY DEGRADING OIL IN A WIDE TEMPERATURE RANGE
Authors: Aitkeldiyeva S.А. , Tatarkina L.G. , Baimakhanova B.G. , Spankulova G.A. , Smirnova I.E. Smirnova
Published: November 2022

The collection cultures of oil-oxidizing microorganisms were screened for their ability to degrade oil at different temperatures (15 оС, 30 оС, 50 оС). As a result, 10 strains were selected that showed high activity at all studied temperatures.
THE LIPID COMPONENT'S FATTY ACID COMPOSITION OF PROTEIN CONCENTRATE BASED ON METHYLOCOCCUS CAPSULATUS GSB-15
Authors: Nikitin N.S. , Trebukh M.D.
Published: November 2022

The results of analysis of fatty acid composition of lipid component of a new type of food product - protein concentrate obtained from biomass of methanoxidizing bacteria Methylococcus capsulatus, strain GSB-15 (BK), are presented.
MOLECULAR GENETIC IDENTIFICATION OF RAW MATERIAL DERIVED FROM THE INSECT HERMETIA ILLUCENS
Authors: Rjabinina U.O. , Shestakova S.I. , Trebuкh M.D. , Nikitin N.S.
Published: November 2022

A method for H.Illucens identification based on real time polymerase chain reaction (PCR-RT) by TaqMan technology has been developed.
RESULTS OF GMO SCREENING IN SOME TYPES OF FOOD
Authors: Trebukh M.D. , Shestakova S.I. , Nikitin N.S.
Published: November 2022

The publication presents the selective control's results over GMO in specialized food products and dietary supplements, conducted by the FSBSI «FRC of Nutrition, Biotechnology» in 2011-2022.
IMPROVEMENT OF IMMUNOLOGICAL RESEARCH METHODOLOGY IN THE SYSTEM OF NOVEL FOOD SAFETY ASSESSMENT
Authors: Nikitin N.S. , Shestakova S.I. , Sadykova E.O.
Published: November 2022

Immunological studies have an important role in the comprehensive safety assessment of novel food. This publication presents the data of the experiment with different doses of ovalbumin, that confirms the informative value of the study of morphological indicators of the immune system’s organs (thymus, spleen, Payer's patch).
BIOCATALYSIS AND THERMOPLASTIC EXTRUSION IN THE TECHNOLOGY OF READY-TO-EAT PRODUCTS USING FRUIT AND BERRY POMACE
Authors: Sokolova E.N. , Volkova G.S. , Yuraskina T.V. , Amelyakina M.V.
Published: November 2022

The effect of enzymes with different substrate specificity in the processing of fruit and berry raw materials on the chemical composition of its pomaces was studied. Extrusion technology with a degassing system can be used as an efficient way of utilization of pomaces processing it mixed with cereals into ready-to-eat products with high nutritional value.
DEVELOPMENT OF APPROACHES TO THE NOVEL FOOD OBTAINED FROM MICROBIAL SYNTHESIS SAFETY ASSESSMENT: FEATURES OF EXPERIMENTAL PLANNING
Authors: Sadykova E.O. , Stankevich A.A. , Shumakova A.A.
Published: November 2022

Based on the available scientific advance and the latest concepts in the field of toxicology, medicine, genomic and proteomic technologies, approaches to the safety assessment of food obtained through microbial synthesis have been developed.
INHIBITOR OF APOPTOSIS XIAP AND GLIOBLASTOMA CELLS RESISTANCE TO VACCINIA VIRUS VV-GMCSF-LACT
Authors: Ageenko A.B. , Chinak O.A. , Richter V.A. , Kuligina E.V.
Published: November 2022

It has been shown that under the action VV-GMCSF-Lact, the death of glioblastoma cells occurs mainly through the apoptotic pathway. When analyzing the complexes of Smac protein with the apoptosis inhibitor XIAP in human glioblastoma cells, it was found that in the cells most sensitive to VV-GMCSF-Lact, Smac level was increased after incubation with the virus. At the same time, overall level of XIAP was higher in more resistant cells.
DEVELOPMENT OF TECHNOLOGY FOR OBTAINING CHIMERIC ANTIGEN- CARRIER PROTEIN FOR PEPTIDE VACCINES PRODUCTION
Authors: Esina T.I. , Gogina Ya.S. , Tereshenko T.A. , Sarpova A.I. , Levagina G.M. , Danilenko E.D.
Published: November 2022

A scheme for obtaining a highly purified carrier protein of peptide antigens of the vaccine against a new coronavirus infection COVID-19 has been worked out. The developed technology has been scaled up. The scaling-up makes it possible to obtain up to 15-20 mg of pure protein from 1 g of wet biomass that meets the requirements for drugs obtained by recombinant DNA methods.
CONTROLLING THE EXPRESSION LEVEL OF ALPHA-DEFENSINS BY LOW MOLECULAR WEIGHT BIOREGULATORS
Authors: Kolesnikova N.V. , Shvydchenko I.N. , Ovchinnikova T.V.
Published: November 2022

Human alpha defensins (Human neutrophil peptides 1-3, HNP1-3) are constitutively produced by immunocompetent cells and protect against pathogenic bacteria. The effect of low molecular weight bioregulators catecholamine norepinephrine and muramyl peptide on the expression of HNP1-3 was studied. It has been shown that muramylpeptide restores the level of alpha-defensins HNP1-3 produced by human neutrophils in vitro, reduced by the action of catecholamine norepinephrine.
SYNTHESIS OF DERIVATIVES Α-LIPOIC ACID WITH GLUTATHIONE
Authors: Shchelkonogov V.A. , Baranova O.A. , Chekanov A.V. , Shastina N.S. , Solov’eva E.Yu. , Fedin A.I.
Published: November 2022

Synthesis of new derivatives of α-lipoic acid (α-LA) with glutathione was carried out to increase their therapeutic effect, protect against biodegradation and prolonged release of α-LA and glutathione from the obtained compounds these substances.
HETEROLOGOUS EXPRESSION BY AUTOINDUCTION OF THE BISPECIFIC FUSION PROTEIN SRH-DR5-B WITH AFFINITY FOR VEGFR2 AND DR5 RECEPTORS
Authors: Yagolovich A.V. , Artykov A.A. , Vorontsova Y.V. , Dolgikh D.A. , Gasparian M.E.
Published: November 2022

A technique for the expression of the bispecific fusion protein SRH-DR5-B by autoinduction in the E. coli strain BL21(DE3)pLys has been developed. The new technologically simple and economical technique allows to obtain high amounts of active target protein from a soluble cell fraction.
INTERACTION OF LEVOFLOXACIN AND IT’S COMPLEXES WITH CYCLODEXTRINS WITH LIPOSOMAL BILAYER AND CELL’S SURFACES OF ESCHERICHIA COLI AND LACTOBACILLUS FERMENTUM
Authors: Yakupova L.R. , Skuredina A.A. , Tychinina A.S. , Le-Deygen I.M. , Belogurova N.G. , Kudryashova E.V.
Published: November 2022

The effect of two β-cyclodextrin derivatives on the interaction of levofloxacin fluoroquinolone with the liposomal bilayer (liposomes) and cell membranes of L. fermentum and E. coli bacteria was studied. It was found that the complex formation of levofloxacin-cyclodextrin enhances the adsorption of the drug on gram-negative cells.
STUDY THE ROLE OF CYTOCHROME C COMPLEX WITH CARDIOLIPIN IN THE CATALYSIS OF LIPID PEROXIDATION AND THE INITIATION OF APOPTOSIS: CALCULATION OF KINETIC CONSTANTS AND QUANTUM YIELDS BASED ON THE KINETICS OF ACTIVATED CHEMILUMINESCENCE
Authors: Vladimirov G.K. , Volodyaev I.V.
Published: November 2022

Cytochrome c in a cell, in an aqueous medium or in a non-polar environment can be both in a free state and in complex with cardiolipin. In the latter case, it is partially denatured, characterized by a specific conformation, has peroxidase activity and is an important component of proapoptotic signaling pathways [1,2,9]. Differences between native cytochrome c and the cytochrome c—cardiolipin complex are recorded by the absorption and fluorescence spectra, as well as by the pronounced peroxidase activity of the latter and chemiluminescence accompanying the free radical processes it triggers. Based on this, software was developed for automatic calculation of kinetic parameters and quantum yields, lipid peroxidation of activated chemiluminescence and visualization of model curves.
MATHEMATICAL MODELING OF QUANTUM YIELDS OF COUMARIN- ACTIVATED C-314 CHEMILUMINESCENCE UNDER THE ACTION OF CYTOCHROME C COMPLEX WITH CARDIOLIPIN
Authors: Volodyaev I.V. , Vladimirov G.K.
Published: November 2022

The current work is devoted to mathematical modeling of the processes of oxidative degradation of lipids and its accompanying chemiluminescence (CL) triggered by the CytC-CL complex in the membrane. The formation of a genuine model of this process allows the use of CL methods as a way to correctly assess the enzymatic activity of CytC-CL, that is, to thoroughly investigate its behavior under various conditions.
MONOCLONAL ANTIBODIES TO RECOMBINANT BRAIN-DERIVED NEUROTROPHIC FACTOR: PROSPECTS FOR USE IN CLINICAL AND LABORATORY DIAGNOSTICS
Authors: Pavlov K.A. , Gurina O.I. Gurina
Published: November 2022

Quantitative determination of the brain-derived neurotrophic factor can serve as an important factor in the detection of latent depressions and a marker of the effectiveness of the selected therapy. As a result of immunization with recombinant brain-derived neurotrophic factor, mono- and polyclonal antibodies were obtained. The test system for quantitative enzyme immunoassay on the basis of these components has been developed. It can be used to determine serum levels of this antigen in depressive patients.
CATALYTIC ACTIVITY OF RECOMBINANT LYSOSTAPHIN PURIFIED ON VARIOUS RESINS
Authors: Grishin A.V. , Lyashchuk A.M. , Karyagina A.S. , Lunin V.G.
Published: November 2022

A variant of the antibacterial enzyme lysostaphin with a histidine tag has been isolated and purified on various sorbents. A significant effect of the sorbent used for the purification of lysostaphin on its catalytic activity was shown.
MASS SPECTROMETRIC DIAGNOSIS OF RECOVERY AFTER RESPIRATORY ILLNESS USING MACHINE LEARNING METHODS
Authors: Kuzmin A.G. , Titiov Y.A. , Zaitceva A.Y.
Published: November 2022

The express methodology of evaluation of mass-spectrometric parameters of gas composition of exhaled air for differential diagnostics of acute respiratory viral infections and tracing of dynamics of recovery after the disease was developed.
AN IN VITRO ANGIOGENESIS MODEL BASED ON CELLULAR SPHEROIDS OBTAINED FROM CO-CULTURED ENDOTHELIOCYTES AND MESENCHYMAL STROMAL CELLS
Authors: Tsvetkova A.V. , Novikova V.D. , Karalkin P.A. , Rodionov S.A. , Sergeeva N.S. , Yarygin K.N.
Published: November 2022

The formation of vascular outgrowths (sprouts) from cellular spheroids based on endotheliocytes and mesenchymal stromal cells was demonstrated to take place upon culturing the spheroids in a three-dimensional fibrin gel. The results can serve as a basis for a new angiogenesis model of a high biological relevance.
ACADEMICAL APPROACH TO DEVELOPMENT OF ANTICANCER AGENTS
Authors: Korolchuk A.M.
Published: November 2022

Design and synthesis of several series of steroid derivatives - potential inhibitors of CYP17A1 was carried out. Two leader compounds with high pharmacological potential were receveived.
INFLUENCE OF STEROID ISOXAZOLES ON THE EXPRESSION OF KEY GENES OF THE SONIC HEDGEHOG CASCADE IN NORMAL AND CANCER CELLS
Authors: Alexandrova A.A. , Zolotsev V.A. , Timoshenko O.S. , Zavyalova M.G. , Sanzhakov M.A. , Shcherbakov K.A. , Rudovich A.S. , Zhabinskii V.N. , Khripach V.A.
Published: November 2022

In the scientific world, the study of Shh-signaling is one of the hot topics for research, as it concerns socially significant diseases such as cancer, Shh-signaling is one of the main types of signaling in embryonic and tumor cells. Studies of Shh-signaling can provide information about new molecular targets, ideas for creating new drugs, and the possibility of combating drug resistance. Steroid molecules are directly related to the mechanisms of regulation of Shh-signaling activation. It has been suggested that steroids similar in structure to cholesterol or cyclopamine may be antagonists or agonists of Shh-signaling.
University-Community Partnership Model to Support Rural STEM Teaching and Student Engagement
Authors: Kavanagh Kathleen , DeWaters Jan , Rivera Seema , Richards Melissa , Ramsdell Michael , Galluzzo Ben
Published: November 2022

Rural economically disadvantaged communities face unique challenges in engaging students in science, technology, engineering, and mathematics (STEM). School district administrators, teachers, and students do not have access to high-quality STEM opportunities compared to urban schools. This article describes a partnership between a small, private STEM university and a network of school districts scattered across the geographically isolated region of upstate New York. The partnership’s primary goal is to support the teaching and learning of STEM. This is achieved through actively engaging a range of university and community stakeholders in STEM enrichment and professional development. Programming includes summer camps and after-school activities, challenges and competitions that focus on inspiring students to pursue STEM careers, undergraduate and graduate student mentors, and a university curriculum designed to prepare teachers to work in high-need school districts. Activities are supported by the university’s Institute for STEM Education, which fosters collaborations for like-minded faculty and campus members to pursue grant opportunities and connect with community members. The paper describes various program components and how they work to support each other, discusses impacts of the program, and describes ways in which elements can be implemented elsewhere.