bluuebunny/crossref_metadata_2025 huggingface dataset:
The big picture: Representation of LGBTQ characters and themes in picture books available in the United States 1972-2018
Authors: Izienicki Hubert
Published: December 2022

The number of LGBTQ picture books—literary works for children containing lesbian, gay, bisexual, trans, and queer characters and themes—has increased considerably since 2000. While different segments of this category of books have been examined, there has been a limited examination of the entire genre. Using 234 English-language LGBTQ picture books available in the United States between 1972 and 2018, I conducted a content analysis of the main themes and central characters as well as investigate how the main themes changed over time and the extent to which they reflected the larger historical contexts in which they were created. I find that very few LGBTQ characters are cast as main protagonists and some (i.e., bisexuals) are completely absent. Similarly, I find an increase in diversity of themes over the decades yet with most centering on marriage, parenting, and domesticity. Together, LGBTQ picture books convey a limited view of the LGBTQ lives.
Constructions of self and the pursuit of ‘authenticity’ in women’s magazines: A study of British and Greek discourses
Authors: Orphanides Rafaela , Nyhagen Line , Keightley Emily
Published: December 2022

This article examines discourses of authenticity embedded in European popular culture based on an empirical study of British and Greek women’s magazines. After a quantitative content analysis of 575 articles published in Greek and British editions of Cosmopolitan and Marie Claire during 2012–2016, we conducted a qualitative repertoire analysis of 80 articles and identified a shared discursive repertoire, that of women’s ‘authentic self’. Our analysis suggests that discourses of authenticity in women’s magazines contain contradictory elements. First, they are characterised by a ‘can do’ philosophy, an emphasis on ‘body-positivity’, ‘self-acceptance’ and self-help advice, which suggests women’s agency as well as a normalisation of postfeminist gender anxieties. Second, the discourses encourage women to accept their bodies, ‘internal selves’ and current circumstances through self-monitoring and self-surveillance, while silencing societal barriers that form obstacles to achieving ‘an authentic self’. These authenticity discourses rely on a triple entanglement of notions derived from third-wave feminism, post-feminism and neoliberal discourse.
Research on incremental nonlinear dynamic inverse control method for turboshaft engine based on Jacobi matrix
Authors: Song Jie , Wang Yong , Li Shancheng , Zhang Haibo
Published: December 2022

In order to enhance the anti-interference ability of the speed to external load and achieve the rapid response control for turboshaft engine of next-generation helicopters, an incremental nonlinear dynamic inverse (INDI) control method for turboshaft engine based on Jacobi matrix is proposed. First, the control law of turboshaft engine based on state variable model (SVM) is obtained according to the mathematical derivation of INDI. Then, based on adopting Jacobi matrix and sequential quadratic programming (SQP) algorithm, the SVM is online obtained, so as to express the steady and dynamic characteristics of turboshaft engines under different flight conditions precisely. Finally, several numerical simulations are conducted to validate the control effect of INDI control method. The results demonstrate that compared with cascade PID controller, INDI control method based on single SVM can significantly decrease the overshoot and sag of power turbine speed by more than 50% under constant flight conditions. Under variable flight conditions, INDI control method based on Jacobi matrix can effectively decrease the overshoot and sag of power turbine speed by more than 30% compared with INDI control method based on single SVM, which has more significant control effect and superior robustness, and realizes the fast response control of turboshaft engines.
A Case of Pharmaceutical Messianism Amidst the COVID-19 Pandemic: An Infodemiological Study of Ivermectin in the Philippines
Authors: Alibudbud Rowalt
Published: December 2022

Pharmaceutical messianism is a manifestation of medical populism. It arises during extraordinary crises, is built on the familiar, endorsed by heterodox authorities, and involves a highly accessible panacea. Amid the politics and public desperation in the Philippines during the COVID-19 pandemic, pharmaceutical messianism can be observed in the form of Ivermectin, a panacea offered to prevent and treat COVID-19. Thus, it may be worthwhile to determine the changes and patterns of public interest toward Ivermectin. This infodemiological study utilized and described Search Volume Index and related queries for Ivermectin from Google Trends vis-à-vis reported societal events in the Philippines to determine changes in public interest in Ivermectin use. It revealed that a tremendous increase in public interest in Ivermectin has emerged during surges of COVID-19 cases, endorsement by politicians and heterodox health authorities, and public distribution of Ivermectin. It also showed that public interest increased as the number of component characteristics of pharmaceutical messianism increased. Search-related queries and topics also showed that the public might be using the internet to inform themselves regarding the use of Ivermectin for humans, including its use for COVID-19. These findings suggest that people may study the endorsed panacea and weigh it against conventional and orthodox treatment during rising COVID-19 cases. Thus, easily understandable, highly searchable, reliable, and trustworthy online information is ever-crucial in this age of information and disinformation.
High-Density (HD) Scalp EEG Findings in “Benign” Childhood Epilepsy with Centrotemporal Spikes (BCECTS)
Authors: Bhatia Sonal , Ham Andrew Todd , Kutluay Ekrem
Published: December 2022

Despite characteristic clinical and scalp EEG findings, BCECTS pathophysiology is unclear regarding involvement of large-scale neuronal networks. Higher number of scalp electrodes with HD-EEG may promote accurate localization of the cortical generators in BCECTS providing additional insight in those with neurocognitive problems. We aimed to determine the value of visual interpretation of topographical maps using 256 channels (when compared to standard 21 channel array) HD-EEG in BCECTS and attempted to source localize interictal discharges (IEDs) using Geosource 2 software. Patient records were reviewed for demographic, seizure, brain magnetic resonance imaging (MRI) details; scalp and HD-EEG findings. HD-EEG software was used to review raw EEG data (21 scalp EEG electrodes were compared to 256 HD-EEG electrodes); select, average, and source localize IEDs.

Five BCECTS patients with HD-EEG were identified. Seizure onset age ranged from 5–11 years with 1–18 lifetime seizures; both focal ( n = 3) and focal to bilateral tonic-clonic ( n = 2). Neurocognitive co-morbidities noted in our cohort included attention deficit hyperactivity disorder, speech/developmental delay and a specific learning disorder. Scalp EEG showed typical findings with IEDs over the centrotemporal regions (bilateral n = 3; unilateral n = 2). Visualization and inspection of expanded coverage topographic maps with HD-EEG showed well-defined islands of maximum negativity and positivity of a dipole compared to conventional channels where boundary delineation was obscured. Further, HD-EEG localized IEDs/“spike-generator” to areas such as the pre-and post-central, middle-frontal and temporal gyrus, and the inferior parietal lobule. In BCECTS, HD-EEG may show affection for a broader neural network and may provide a better insight into the associated neurocognitive morbidities.
Effectiveness and safety of salmeterol/fluticasone fixed-dose combination delivered through Synchrobreathe® in patients with asthma: the real-world EVOLVE study
Authors: Balamurugan Santhalingam , Sonia Dalal , Vikrant Deshmukh , Monotosh Khanra , Raj Shyam Sundar , Shamim Akhtar , Vinay Kumar , Velayuthaswamy Nandagopal , Masood Ahmed , Manohar Lal Gupta , Ajay Godse , Sushmeeta Chhowala , Meena Lopez , Sandesh Sawant , Sonali Jadhav , Abhijit Vaidya , Jaideep Gogtay
Published: December 2022

Background:

Inhalation therapy with corticosteroids and long-acting β2-agonists has been the mainstay of asthma management. However, choosing the correct inhaler technique is essential to effectively deliver the medication to the lungs to attain good asthma control.

Objective:

This study aimed to evaluate asthma control and device usability with salmeterol/fluticasone fixed-dose combination (FDC) administered through Synchrobreathe®, a breath-actuated inhaler (BAI), in Indian patients with persistent asthma (EVOLVE study).

Design:

The present study was a prospective, open-label, non-comparative, multi-center, observational study.

Methods:

The study enrolled 490 patients with documented diagnoses of asthma who were treatment-naive or uncontrolled due to poor inhaler technique associated with a previous device. The primary endpoint was a change from baseline in the Asthma Control Questionnaire-6 (ACQ-6) score at week 12.

Results:

Mean ACQ-6 score reduced from 2.2 ± 1.07 (baseline) to 0.4 ± 0.49 (mean change: –1.9 ± 1.12, p < 0.0001) at week 12 in the intention-to-treat (ITT) population, and minimal clinically important difference of 0.5 was observed from week 4 onwards. Peak expiratory flow rate improved by 82.5 ± 75.74 ml/min ( p < 0.0001) at week 12 in the ITT population. The proportion of well-controlled responders increased from 39.9% (week 4) to 77.1% (week 12). Most (91%) patients preferred the Synchrobreathe® and rated it very high for usability, portability, patient confidence, and satisfaction. Salmeterol/fluticasone FDC administered through Synchrobreathe® was well tolerated.

Conclusion:

Treatment with salmeterol/fluticasone FDC administered through Synchrobreathe® for 12 weeks persistently improved asthma control and lung function and was well tolerated. Most patients were satisfied with it and preferred Synchrobreathe® BAI over their previous device.

Registration:

The study was registered with the Clinical Trial Registry of India (CTRI/2018/12/016629).
Think of What Really Matters: Structured Analysis of Personal Criteria can Save Lives
Authors: Gordon-Hecker Tom , Kogut Tehila
Published: December 2022

Helping decisions are susceptible to many biases—partly due to the helpers’ spontaneous emotional reactions to the appeal diverting their attention from the need to maximize the impact of their help. Attempts to overcome these biases by prompting deliberative thinking—namely, by asking participants to think deeply—have often been unsuccessful. Here, we propose a way of directing people’s attention to the most important aspects of their decisions, by asking them to rate the extent to which such attributes should be considered. In two experiments involving real-world crises, participants who underwent such structured analysis of their personal criteria were more likely to make decisions that maximized the number of lives saved. Moreover, their decisions were more in line with their personal values. We conclude that this method is a simple, efficient way of improving the quality of helping decisions in life-and-death situations.
Understanding coronavirus disease 2019 (COVID-19) vaccine hesitancy: Evidence from the community-driven knowledge site Quora
Authors: Jang Sou Hyun , Gerend Mary A. , Youm Sangpil , Yi Yong J
Published: December 2022

Objective

The present study aims to examine the threshold of coronavirus disease 2019 (COVID-19) vaccine hesitancy over time and public discourse around COVID-19 vaccination hesitancy.

Methods

We collected 3,952 questions and 66,820 answers regarding COVID-19 vaccination posted on the social question-and-answer website Quora between June 2020 and June 2021 and employed Word2Vec and Sentiment Analysis to analyze the data. To examine changes in the perceptions and hesitancy about the COVID-19 vaccine, we segmented the data into 25 bi-weekly sections.

Results

As positive sentiment about vaccination increased, the number of new vaccinations in the United States also increased until it reached a ceiling point. The vaccine hesitancy phase was identified by the decrease in positive sentiment from its highest peak. Words that occurred only when the positive answer rate peaked (e.g., safe, plan, best, able, help) helped explain factors associated with positive perceptions toward vaccines, and the words that occurred only when the negative answer rate peaked (e.g., early, variant, scientists, mutations, effectiveness) suggested factors associated with vaccine hesitancy. We also identified a period of vaccine resistance, where people who decided not to be vaccinated were unlikely to be vaccinated without further enforcement or incentive.

Conclusions

Findings suggest that vaccine hesitancy occurred because concerns about vaccine safety were high due to a perceived lack of scientific evidence and public trust in healthcare authorities has been seriously undermined. Considering that vaccine-related conspiracy theories and fake news prevailed in the absence of reliable information sources, restoring public trust in healthcare leaders will be critical for future vaccination efforts.
Mature aged Aboriginal and Torres Strait Islander adults are using digital health technologies (original research)
Authors: Henson Connie , Chapman Felicity , Shepherd Gina , Carlson Bronwyn , Chau Josephine Y , Gwynn Josephine , McCowen Deb , Rambaldini Boe , Ward Katrina , Gwynne Kylie
Published: December 2022

Background

Aboriginal and Torres Strait Islander people avidly use technology for a variety of purposes. Digital health technologies offer a new way to build on Aboriginal and Torres Strait Islander peoples propensity for early adoption and innovation with technology. Only limited research has focused on mature aged adults in non-urban locations as partners in digital health research and there is no research related to wearables for health tracking for this cohort.

Objective

This paper provides insights into mature aged Aboriginal and Torres Strait Islander adults interest, use and trust of social media, apps and wearables to gain health information and manage health.

Methods

This cross-sectional survey study was co-designed and co-implemented with Aboriginal Community Controlled Health Services (ACCHS) in three locations in New South Wales, Australia. The 13-item survey was administered via a semi-structured interview.

Results

Aboriginal and Torres Strait Islander adults ( n = 78), in regional, rural and remote locations indicated their interest in and use of apps and wearables for health purposes. Mature aged participants, particularly women, used Facebook, ACCHS websites and YouTube for acquiring health-related information which they then shared online and in real life with a diversity of family, friends and colleagues.

Conclusions

Aboriginal and Torres Strait Islander people are using digital health technologies to acquire and share health information and want to use apps and wearables for health management. Co-designed research enables a greater understanding of the diverse needs for different cohorts and informs culturally responsible design. Broader use of co-design will foster effective user-focused digital health communication and health-management.
Safety for Whom? Investigating How Platforms Frame and Perform Safety and Harm Interventions
Authors: Gillett Rosalie , Stardust Zahra , Burgess Jean
Published: December 2022

This article reports on a thematic content analysis of 486 newsroom posts published between 2016 and 2021 by five prominent digital platforms (Facebook, Tinder, YouTube, TikTok, and Twitter). We aimed to understand how these platforms frame and define the issues of harm and safety, and to identify the interventions they publicly report introducing to address these issues. We found that platforms respond to and draw upon external controversies and media panics to selectively construct matters of concern related to safety and harm. They then reactively propose solutions that serve as justification for further investment in and scaling up of automated, data-intensive surveillance and verification technologies. We examine four key themes in the data: locating harm with bad actors and discrete content objects (Theme 1), framing surveillance and policing as solutions to harm (Theme 2), policing “borderline” content through suppression strategies (Theme 3), and performing diversity and inclusion (Theme 4).
Clostridioides difficile Induced Rhabdomyolysis Associated With Decompensated Cirrhosis
Authors: Dungan William , Young Gabrielle , Collins Bradley , Romano John , Honko Nicholas , Rockey Don
Published: December 2022

Rhabdomyolysis (RBD) occurs secondary to tissue injury, resulting in (muscle) cell lysis and release of intracellular electrolytes and proteins into circulation. An elevation in the muscle enzyme, creatine kinase (CK), is a diagnostic marker and indicates muscle breakdown. Symptoms include dark urine caused by release of myoglobin, myalgias, and acute kidney injury (AKI). RBD is categorized as (1) traumatic, (2) nontraumatic exertional (ie, metabolic myopathies), or (3) non-exertional and non-traumatic. Clostridioides difficile (CD) has been previously reported to cause RBD, but the risk factors, pathogenesis, and recommended treatment regimen remain unclear.
Respiratory Failure With Aspergillus submaranianii and Lymphoplasmacystic Vasculitis
Authors: D’Assumpcao Carlos , Heidari Arash
Published: December 2022

Aspergillus subramanianii is a very rare form of Aspergillus in section Circumdati. Aspergillus subramanianii has been rarely reported or ever published. IgG4 disease is an emerging disease entity that can be a diagnostic challenge. We report a critically ill patient who had invasive A subramanianii pulmonary aspergillosis and posthumous diagnosis of IgG subclass 4 interstitial lung disease.
Modification of a Method for Diagnosing Noise-Induced Hearing Loss Sustained During Military Service
Authors: Moore Brian C.J. , Humes Larry E. , Cox Graham , Lowe David , Gockel Hedwig E.
Published: December 2022

Moore (2020) proposed a method for diagnosing noise-induced hearing loss (NIHL) sustained during military service, based on an analysis of the shapes of the audiograms of military personnel. The method, denoted M-NIHL, was estimated to have high sensitivity but low-to-moderate specificity. Here, a revised version of the method, denoted rM-NIHL, was developed that gave a better balance between sensitivity and specificity. A database of 285 audiograms of military noise-exposed men was created by merging two previously used databases with a new database, randomly shuffling, and then splitting into two, one for development of the revised method and one for evaluation. Two comparable databases of audiograms of 185 non-exposed men were also created, again one for development and one for evaluation. Based on the evaluation databases, the rM-NIHL method has slightly lower sensitivity than the M-NIHL method, but the specificity is markedly higher. The two methods have similar overall diagnostic performance. If an individual is classified as having NIHL based on a positive diagnosis for either ear, the rM-NIHL method has a sensitivity of 0.98 and a specificity of 0.63. Based on a positive diagnosis for both ears, the rM-NIHL method has a sensitivity of 0.76 and a specificity of 0.95.
The Development and Pilot Study of a Multiple Criteria Decision Analysis (MCDA) to Compare Patient and Provider Priorities around Amputation-Level Outcomes
Authors: Poehler Diana , Czerniecki Joseph , Norvell Daniel , Henderson Alison , Dolan James , Devine Beth
Published: December 2022

Background. Patients with chronic limb-threatening ischemia who are facing a lower-limb amputation often require a transmetatarsal amputation (TMA) or a transtibial amputation (TTA). A TMA preserves more of the patient’s limb and may provide better mobility but has a lower probability of primary wound healing relative to a TTA and may result in additional amputation surgeries. Understanding the differences in how patients and providers prioritize key outcomes may enhance the amputation decisional process. Purpose. To develop and pilot test a multiple criteria decision analysis (MCDA) tool to elicit patient values around amputation-level selection and compare those with provider perceptions of patient values. Methods. We conducted literature reviews to identify and measure the performance of criteria important to patients. Because the quantitative literature was sparse, we developed a Sheffield elicitation framework exercise to elicit criteria performance from subject matter experts. We piloted our MCDA among patients and providers to understand tool acceptability and preliminarily assess differences in patient and provider priorities. Results. Five criteria of importance were identified: ability to walk, healing after amputation surgery, rehabilitation intensity, limb length, and prosthetic/orthotic device ease. Patients and providers successfully completed the MCDA and identified challenges in doing so. We propose potential solutions to these challenges. The results of the pilot test suggest differences in patient and provider outcome priorities. Limitations. The pilot test study enrolled a small sample of providers and patients. Conclusions. We successfully implemented the pilot study to patients and providers, received helpful feedback, and identified solutions to improve the tool. Implications. Once modified, our MCDA tool will be suitable for wider rollout.

Highlights

Patients and providers have successfully completed our MCDA, and patients feel the MCDA may be useful in clinical practice. We encountered several methodologic challenges and identified approaches to ease participant burden. When data are sparse, using the Sheffield elicitation framework is helpful in creating a performance matrix, although patients relied largely on their amputation experiences to complete the exercise. Blinding the alternatives may help patients better understand the process.
Cryptozoologica: Developing the Child as Performer
Authors: White Rachel , Coyle Jim
Published: December 2022

Music, movement, and imaginative play are essential for young children to explore and understand the world around them. It is important for educators of early primary students to consider how they can facilitate meaningful learning experiences that are practical, engaging, and allow children to investigate unfamiliar musical ideas in supportive, fun environments. This article introduces Cryptozoologica, a new work for flute, viola, and cello with Storyteller by Australian composer Jim Coyle, and describes how educators of young children can use music from a range of cultures and genres, including Western art music, to teach students to physically, cognitively, and imaginatively engage with sounds and stories. It includes the full score of Cryptozoologica and five lessons that encourage listening, movement, and play as a means of embodying musical understanding and knowledge.
Effect of green cardamom on the expression of genes implicated in obesity and diabetes among obese women with polycystic ovary syndrome: a double blind randomized controlled trial
Authors: Cheshmeh Sahar , Elahi Negin , Ghayyem Maysa , Mosaieby Elaheh , Moradi Shima , Pasdar Yahya , Tahmasebi Susan , Moradinazar Mehdi
Published: December 2022

Background

Polycystic ovary syndrome (PCOS) is an endocrine disease in which related to obesity, metabolic disorders and is considered as one of the main causes of infertility in women. This trial was investigated the effects of green cardamom on the expression of genes implicated in obesity and diabetes among obese women with PCOS.

Methods

One hundred ninety-four PCOS women were randomly divided two groups: intervention (n = 99; 3 g/day green cardamom) and control groups (n = 95). All of them were given low calorie diet. Anthropometric, glycemic and androgen hormones were assessed before and after 16-week intervention. The reverse transcription-polymerase chain reaction (RT-PCR) method was used to measure fat mass and obesity-associated (FTO), peroxisome proliferative activating receptor- (PPAR-), carnitine palmitoyltransferase 1A (CPT1A), acetyl-CoA carboxylase beta (ACAB), leptin receptor (LEPR), ghrelin, and lamin A/C (LAMIN) genes expression in each group.

Results

Anthropometric indices were significantly decreased after intervention in both two studied groups. Glycemic indices and androgen hormones were significantly improved in the intervention group compared to the control group. The expression levels of FTO, CPT1A, LEPR, and LAMIN were significantly downregulated compared to control group (P < 0.001), as well as, PPAR-y was significantly upregulated in the intervention group after intervention with green cardamom compared to control group (P < 0.001).

Conclusion

This current study showed that the administration of green cardamom is a beneficial approach for improving anthropometric, glycemic, and androgen hormones, as well as obesity and diabetes genes expression in PCOS women under the low-calorie diet.

Trial registration

This trial was registered with the Iranian Clinical Trials Registry (registration number: IRCT20200608047697N1). 1 August, 2020; https://www.irct.ir/trial/48748
Genome-wide association mapping and genomic prediction of agronomical traits and breeding values in Iranian wheat under rain-fed and well-watered conditions
Authors: Rabieyan Ehsan , Bihamta Mohammad Reza , Moghaddam Mohsen Esmaeilzadeh , Mohammadi Valiollah , Alipour Hadi
Published: December 2022

Background

The markers detected by genome-wide association study (GWAS) make it possible to dissect genetic structure and diversity at many loci. This can enable a wheat breeder to reveal and used genomic loci controlling drought tolerance. This study was focused on determining the population structure of Iranian 208 wheat landraces and 90 cultivars via genotyping-by-sequencing (GBS) and also on detecting marker-trait associations (MTAs) by GWAS and genomic prediction (GS) of wheat agronomic traits for drought-tolerance breeding. GWASs were conducted using both the original phenotypes (pGWAS) and estimated breeding values (eGWAS). The bayesian ridge regression (BRR), genomic best linear unbiased prediction (gBLUP), and ridge regression-best linear unbiased prediction (rrBLUP) approaches were used to estimate breeding values and estimate prediction accuracies in genomic selection.

Results

Population structure analysis using 2,174,975 SNPs revealed four genetically distinct sub-populations from wheat accessions. D-Genome harbored the lowest number of significant marker pairs and the highest linkage disequilibrium (LD), reflecting different evolutionary histories of wheat genomes. From pGWAS, BRR, gBLUP, and rrBLUP, 284, 363, 359 and 295 significant MTAs were found under normal and 195, 365, 362 and 302 under stress conditions, respectively. The gBLUP with the most similarity (80.98 and 71.28% in well-watered and rain-fed environments, correspondingly) with the pGWAS method in the terms of discovered significant SNPs, suggesting the potential of gBLUP in uncovering SNPs. Results from gene ontology revealed that 29 and 30 SNPs in the imputed dataset were located in protein-coding regions for well-watered and rain-fed conditions, respectively. gBLUP model revealed genetic effects better than other models, suggesting a suitable tool for genome selection in wheat.

Conclusion

We illustrate that Iranian landraces of bread wheat contain novel alleles that are adaptive to drought stress environments. gBLUP model can be helpful for fine mapping and cloning of the relevant QTLs and genes, and for carrying out trait introgression and marker-assisted selection in both normal and drought environments in wheat collections.
Chromosome-level genome sequence of the Genetically Improved Farmed Tilapia (GIFT, Oreochromis niloticus) highlights regions of introgression with O. mossambicus
Authors: Etherington G. J. , Nash W. , Ciezarek A. , Mehta T. K. , Barria A. , Peñaloza C. , Khan M. G. Q. , Durrant A. , Forrester N. , Fraser F. , Irish N. , Kaithakottil G. G. , Lipscombe J. , Trong T. , Watkins C. , Swarbreck D. , Angiolini E. , Cnaani A. , Gharbi K. , Houston R. D. , Benzie J. A. H. , Haerty W.
Published: December 2022

Background

The Nile tilapia (Oreochromis niloticus) is the third most important freshwater fish for aquaculture. Its success is directly linked to continuous breeding efforts focusing on production traits such as growth rate and weight. Among those elite strains, the Genetically Improved Farmed Tilapia (GIFT) programme initiated by WorldFish is now distributed worldwide. To accelerate the development of the GIFT strain through genomic selection, a high-quality reference genome is necessary.

Results

Using a combination of short (10X Genomics) and long read (PacBio HiFi, PacBio CLR) sequencing and a genetic map for the GIFT strain, we generated a chromosome level genome assembly for the GIFT. Using genomes of two closely related species (O. mossambicus, O. aureus), we characterised the extent of introgression between these species and O. niloticus that has occurred during the breeding process. Over 11 Mb of O. mossambicus genomic material could be identified within the GIFT genome, including genes associated with immunity but also with traits of interest such as growth rate.

Conclusion

Because of the breeding history of elite strains, current reference genomes might not be the most suitable to support further studies into the GIFT strain. We generated a chromosome level assembly of the GIFT strain, characterising its mixed origins, and the potential contributions of introgressed regions to selected traits.
Genome-wide identification of SNPs associated with body weight in yak
Authors: Jiang Hui , Chai Zhi-Xin , Cao Han-Wen , Zhang Cheng-Fu , Zhu Yong , Zhang Qiang , Xin Jin-Wei
Published: December 2022

Background

The yak is the most important livestock in the Qinghai-Tibet Plateau, and body weight directly affects the economic values of yak. Up to date, the genome-wide profiling of single-nucleotide polymorphisms (SNPs) associating with body weight has not been reported in yak. In the present study, the SNPs in 480 yaks from three breeds were analyzed using the commercial high-density (600 K) yak SNP chips.

Results

The results identified 12 and 4 SNPs potentially associated with body weight in male and female yaks, respectively. Among them, 9 and 2 SNPs showed significant difference in yak body weight between different genotypes at each locus in male and female yaks, respectively. Further exploration found 33 coding genes within the 100 kbp upstream or downstream to the SNP loci, which might be potentially affected by the variation of SNPs. Among them, G protein-coupled receptor kinase 4 (GRK4) might be potentially affected by the SNP AX-174555047, which has been reported to affect the functioning of two body-weight associated hormones (parathyroid hormone, PTH, and adrenomedullin, ADM). Determination of PTH and ADM levels in yak revealed positive relationship between PTH level and body weight, negative relationship between ADM level and body weight along with the variation of AX-174555047 mutation.

Conclusions

These results suggested that the SNP AX-174555047 might potentially affect body weight through mediating GRK4 expression and then PTH and ADM functioning. Thus, the SNP AX-174555047 might be used as a biomarker for molecular breeding of yak. More investigations are required to validate this point.
Effects of neighborhood features on healthy aging in place: the composition and context of urban parks and traditional local coffeeshops in Singapore
Authors: Yi Huso , Ng Shu Tian , Chang Cheng Mun , Low Cheryl Xue Er , Tan Chuen Seng
Published: December 2022

Background

Healthy aging in place is affected by what the neighborhood provides for older adults. The mixed-methods ethnographic study explored the built environmental and contextual effects of urban parks and traditional local coffeeshops (kopitiam) on health practices among older adults in Singapore.

Methods

A door-to-door survey with 497 older adults from 32 residential blocks in a public housing town assessed exercise and smoking. The walking distances from the residential blocks to the facilities were calculated. Regression analysis examined the associations between the distance and rates of exercise and smoking. Ethnographic assessment data contextualized the quantitative findings.

Results

Older adults’ exercise was associated with proximity to an urban park but not traditional local coffeeshops. High rates of smoking were clustered in the housing blocks close to the coffeeshops, which provided casual drinking places with smoking tables. The proximity to the coffeeshops was significantly associated with increased smoking and decreased exercise. A walking distance of 200 m to the park and coffeeshops was found to discriminate the outcomes.

Conclusions

The findings suggested that walking distances of a few blocks influenced health behaviors among older adults. Their smoking habits appeared to be maintained through environmental features and cultural norms attached to the coffeeshops. Policy of urban planning and redevelopment for the aging population needs to consider the socioecology of healthy aging in place.
Characterizing human movement patterns using GPS data loggers in an area of persistent malaria in Zimbabwe along the Mozambique border
Authors: Hast Marisa , Mharakurwa Sungano , Shields Timothy M. , Lubinda Jailos , Searle Kelly , Gwanzura Lovemore , Munyati Shungu , Moss William J.
Published: December 2022

Background

Human mobility is a driver for the reemergence or resurgence of malaria and has been identified as a source of cross-border transmission. However, movement patterns are difficult to measure in rural areas where malaria risk is high. In countries with malaria elimination goals, it is essential to determine the role of mobility on malaria transmission to implement appropriate interventions.

Methods

A study was conducted in Mutasa District, Zimbabwe, to investigate human movement patterns in an area of persistent transmission along the Mozambique border. Over 1 year, a convenience sample of 20 participants/month was recruited from active malaria surveillance cohorts to carry an IgotU® GT-600 global positioning system (GPS) data logger during all daily activities. Consenting participants were tested for malaria at data logger distribution using rapid antigen diagnostic tests and completed a survey questionnaire. GPS data were analyzed using a trajectory analysis tool, and participant movement patterns were characterized throughout the study area and across the border into Mozambique using movement intensity maps, activity space plots, and statistical analyses.

Results

From June 2016–May 2017, 184 participants provided movement tracks encompassing > 350,000 data points and nearly 8000 person-days. Malaria prevalence at logger distribution was 3.7%. Participants traveled a median of 2.8 km/day and spent a median of 4.6 h/day away from home. Movement was widespread within and outside the study area, with participants traveling up to 500 km from their homes. Indices of mobility were higher in the dry season than the rainy season (median km traveled/day = 3.5 vs. 2.2, P = 0.03), among male compared to female participants (median km traveled/day = 3.8 vs. 2.0, P = 0.0008), and among adults compared to adolescents (median total km traveled = 104.6 vs. 59.5, P = 0.05). Half of participants traveled outside the study area, and 30% traveled into Mozambique, including 15 who stayed in Mozambique overnight.

Conclusions

Study participants in Mutasa District, Zimbabwe, were highly mobile throughout the year. Many participants traveled long distances from home, including overnight trips into Mozambique, with clear implications for malaria control. Interventions targeted at mobile populations and cross-border transmission may be effective in preventing malaria introductions in this region.
A Malaysian consensus recommendation for the prevention of influenza in older persons
Authors: Tan Maw-Pin , Sekawi Zamberi , Abdul Manap Roslina , Razali Rizah Mazzuin , Mahadzir Hazlina , Nordin Nordiana , Koh Kar-Chai , Wong Pui-Li , Hasmuk Kejal , Harrun Noor Harzana , Mokhtar Siti Aisah
Published: December 2022

Background

Older persons are at high-risk of developing severe complications from influenza. This consensus statement was developed to provide guidance on appropriate influenza prevention strategies relevant to the Malaysian healthcare setting.

Methods

Under the initiative of the Malaysian Influenza Working Group (MIWG), a panel comprising 11 multi-speciality physicians was convened to develop a consensus statement. Using a modified Delphi process, the panellists reviewed published evidence on various influenza management interventions and synthesised 10 recommendations for the prevention of influenza among the aged population via group discussions and a blinded rating exercise.

Results

Overall, annual influenza vaccination is recommended for individuals aged ≥ 60 years, particularly those with specific medical conditions or residing in aged care facilities (ACFs). There is no preference for a particular vaccine type in this target population. Antiviral agents can be given for post-exposure chemoprophylaxis or when vaccine contraindication exists. Infection control measures should serve as adjuncts to prevent the spread of influenza, especially during Hajj.

Conclusion

This consensus statement presents 10 evidence-based recommendations that can be adopted by healthcare providers to prevent influenza among the aged population in Malaysia. It could also serve as a basis for health policy planning in other lower- and middle-income countries.
Amyloid-β and APOE genotype predict memory decline in cognitively unimpaired older individuals independently of Alzheimer’s disease polygenic risk score
Authors: Tomassen Jori , den Braber Anouk , van der Lee Sven J. , Reus Lianne M. , Konijnenberg Elles , Carter Stephen F. , Yaqub Maqsood , van Berckel Bart N.M. , Collij Lyduine E. , Boomsma Dorret I. , de Geus Eco J.C. , Scheltens Philip , Herholz Karl , Tijms Betty M. , Visser Pieter Jelle
Published: December 2022

Background:

What combination of risk factors for Alzheimer’s disease (AD) are most predictive of cognitive decline in cognitively unimpaired individuals remains largely unclear. We studied associations betweenAPOEgenotype, AD-Polygenic Risk Scores (AD-PRS), amyloid-β pathology and decline in cognitive functioning over time in a large sample of cognitively unimpaired older individuals.

Methods:

We included 276 cognitively unimpaired older individuals (75 ± 10 years, 63% female) from the EMIF-AD PreclinAD cohort. An AD-PRS was calculated including 83 genome-wide significant variants. TheAPOEgene was not included in the PRS and was analyzed separately. Baseline amyloid-β status was assessed by visual read of [18F]flutemetamol-PET standardized uptake value images. At baseline and follow-up (2.0 ± 0.4 years), the cognitive domains of memory, attention, executive function, and language were measured. We used generalized estimating equations corrected for age, sex and center to examine associations betweenAPOEgenotype and AD-PRS with amyloid-β status. Linear mixed models corrected for age, sex, center and education were used to examine associations betweenAPOEgenotype, AD-PRS and amyloid-β status, and their interaction on changes in cognitive functioning over time.

Results:

Fifty-two participants (19%) had abnormal amyloid-β, and 84 participants (31%) carried at least oneAPOEε4 allele.APOEgenotype and AD-PRS were both associated with abnormal amyloid-β status. Increasingly more risk-fullAPOEgenotype, a high AD-PRS and an abnormal amyloid-β status were associated with steeper decline in memory functioning in separate models (all p ≤ 0.02). A model including 4-way interaction term (APOE×AD-PRS×amyloid-β×time) was not significant. When modelled together, bothAPOEgenotype and AD-PRS predicted steeper decline in memory functioning (APOEβ(SE)=-0.05(0.02); AD-PRS β(SE)=-0.04(0.01)). Additionally, when modelled together, both amyloid-β status and AD-PRS predicted a steeper decline in memory functioning (amyloid-β β(SE)=-0.07(0.04); AD-PRS β(SE)=-0.04(0.01)). Modelling bothAPOEgenotype and amyloid-β status, we observed an interaction, in whichAPOEgenotype was related to steeper decline in memory and language functioning in amyloid-β abnormal individuals only (β(SE)=-0.13(0.06); β(SE)=-0.22(0.07), respectively).

Conclusion:

Our results suggest thatAPOEgenotype is related to steeper decline in memory and language functioning in individuals with abnormal amyloid-β only. Furthermore, independent of amyloid-β status other genetic risk variants contribute to memory decline in initially cognitively unimpaired older individuals.
Experiences of mothers and significant others in accessing comprehensive healthcare in the first 1000 days of life post-conception during COVID-19 in rural Uganda
Authors: Nakate Mary-Grace , Mackay Sean , Ndirangu-Mugo Eunice , Fleming Valerie
Published: December 2022

Background

COVID-19 presented an unprecedented global public health challenge because of its rapid and relentless spread, and many countries instituted lockdowns to prevent the spread of infection. Although this strategy may have been appropriate to reduce infection, it presented unintended difficulties in rural Uganda, especially in maternal and born newborn care. For example, some services were suspended, meaning the nearest health facility was at a considerable distance. This study explored the experiences of mothers and their significant others of comprehensive care in the first 1000 days of life post-conception during the COVID-19 pandemic in Bunghokho-Mutoto sub-county, Mbale District, Uganda.

Methods

A qualitative exploratory descriptive design was used with data collected in semi-structured interviews. Mothers (pregnant or with a child under 2 years) and their significant others were purposively recruited for this study. The sample size (N = 14) was determined by data saturation. Data. were analysed using thematic analysis.

Results

One theme emerged “Increasing barriers to healthcare”, which encompassed six sub-themes: accessing healthcare, distressing situations, living in fear, making forced choices, navigating the gatekeepers, and ‘coping with increased poverty.

Conclusion

This study found that the COVID-19 pandemic increased barriers to accessing healthcare services in the region. Participants’ narratives emphasised the lack of access to expert care and the shortage of skilled health workers, especially midwives.
Prognostic significance of preoperative prognostic immune and nutritional index in patients with stage I–III colorectal cancer
Authors: Xie Hailun , Wei Lishuang , Liu Mingxiang , Liang Yanren , Yuan Guanghui , Gao Shunhui , Wang Qiwen , Lin Xin , Tang Shuangyi , Gan Jialiang
Published: December 2022

Background

To explore the value of preoperative prognostic immune and nutritional index (PINI) in predicting postoperative complications and long-term outcomes in patients with stage I–III colorectal cancer (CRC).

Methods

Restricted cubic splines were used to assess the relationship between PINI and survival in patients with CRC. The Kaplan–Meier method and log-rank test were used to plot the survival curves. The Cox proportional hazards model was used to evaluate independent prognostic predictors in patients with CRC. A logistic regression analysis was performed to identify independent predictors of postoperative complications. The least absolute shrinkage and selection operator (LASSO) logistic regression algorithm was used for feature screening.

Results

An evident positive dose–response relationship between PINI and survival in patients with CRC was identified. Compared with patients with a high PINI, those with a low PINI had worse disease-free survival (DFS) (47.9% vs. 66.9%, p < 0.001) and overall survival (OS) (49.7% vs. 70.2%, p < 0.001). The Cox proportional hazards model revealed that PINI was independently associated with DFS (hazard ratio [HR], 0.823; 95% confidence interval [CI], 0.754–0.898; p < 0.001) and OS (HR, 0.833; 95% CI, 0.761–0.912; p < 0.001) in patients with CRC. In the logistic regression analysis, PINI was an independent factor affecting postoperative complications in patients with CRC (odds ratio, 0.710; 95%CI: 0.610–0.810, p < 0.001). The LASSO logistic regression algorithm was used to screen for effective prognostic variables. Finally, we constructed PINI-based nomograms to predict postoperative 1–5-year PFS, and OS in patients with CRC.

Conclusion

PINI is an effective biomarker for predicting postoperative complications, DFS, and OS in patients with stage I–III CRC.
Demographic, Clinical Profile and Outcomes of Neonates Admitted to Neonatal Intensive Care Unit of Dekemhare Hospital, Eritrea
Authors: Gebremariam Hailemichael , Tesfai Berhe , Tewelde Seltene , Abay Samsom , Tekeste Danait , Kibreab Fitsum
Published: December 2022

Background

Ninety-six percent of the world’s 3 million neonatal deaths occur in developing countries where the majority of births occur outside health facility. The objective of this study was to evaluate the demographic, clinical profile and outcome of neonates admitted to Neonatal Intensive Care Unit of Dekemhare Hospital of Eritrea.

Methods

The study was a retrospective register-based review of all neonates admitted from January 2018 to December 2021 to Dekemhare Hospital. Overall, 509 neonates were enrolled in this study. Data were collected from neonatal register book from January 5 to February 5, 2022 by general practitioners using a predesigned data collection tool. Data entry was done using CSpro 7.3 and analyzed through SPSS version 22. Results were presented in frequencies, percent and odds ratio. Univariable and multivariable analysis was done to measure the association between the variables.

Results

Three quarter (75.6%) of the neonates had normal birth weight and 80.0% were term. Majority (75.4%) of the neonates was delivered vaginally and 92.7% were delivered at health facility. Neonatal infection (33.0%), birth asphyxia (20%) and prematurity (14.3%) were the top three primary causes of neonatal admission to the Neonatal Intensive Care Unit. Furthermore, 31% of neonatal deaths occurred during 24-72 hours of their life and the mortality rate was 16.3%. Multivariable analysis indicated that low birth weight (AOR: 7.28; 95%CI: 2.85-18.55) increased neonatal mortality. Whereas delivery at health facility (AOR: 0.17; 95%CI: 0.06–0.47), hospital stay 4-7 days (AOR: 0.06; 95% CI: 0.02-0.23) and above 8 days (AOR: 0.06; 95%CI: 0.02-0.23) were showing protective effect on neonatal mortality.

Conclusion

Congenital abnormality, prematurity and birth asphyxia had higher case fatality rate. And, low birth weight, delivery at health facility and hospital stay were found to be predictors of neonatal mortality. Training of health professionals on neonatal resuscitation, further improvement on the diagnostic setup, treatment tools, infrastructure and raising community awareness to deliver at health facility are crucial to decrease the neonatal mortality in Eritrea.