bluuebunny/crossref_metadata_2025 huggingface dataset:
From Technical Evolution to Production Performance: A Technical Review of 700 Wells Over 8 Years in the South Sulige Tight Gas Field
Authors: Wang Dong , Dong Yifan , Yang Shengfang , Rignol Joel , Wang Qiang , Liu Yuan , Liu Yaolan , Zhao Liang , Wang Weikan , Loh Pengpeng , Enkababian Philippe
Published: January 2022

Unlike many unconventional resources that demonstrate a high level of heterogeneity, conventional tight gas formations often perform consistently according to reservoir quality and the applied completion technology. Technical review over a long period may reveal the proper correlation between reservoir quality, completion technology, and well performance. For many parts of the world where conventional tight gas resources still dominate, the learnings from a review can be adapted to improve the performance of reservoirs with similar features.

South Sulige Operating Company (SSOC), a joint venture between PetroChina and Total, has been operating in the Ordos basin for tight gas since 2011. The reservoir is known to have low porosity, low permeability, and low reservoir pressure, and requires multistage completion and fracturing to achieve economic production.

Over the last 8 years, there has been a clear technical evolution in South Sulige field, as a better understanding of the reservoir, improvement of the completion deployment, optimized fracturing design, and upgraded flowback strategy have led to the continuous improvement of results in this field. Pad drilling of deviated boreholes, multistage completions with sliding sleeve systems, hybrid gel-fracturing, and immediate flowback practices, gradually proved to be the most effective way to deliver the reservoir's potential.

Using the absolute open-flow (AOF) during testing phase for comparative assessment from South Sulige field, we can see that in 2012 this number was 126 thousand std m3/d in 2012, and by 2018 this number had increased to 304 thousand std m3/d, representing a 143% incremental increase. Thus, technical evolution has been proved to bring production improvement over time. Currently, South Sulige field not only outperforms offset blocks but also remains the top performer among the fields in the Ordos basin. The drilling and completion practices from SSOC may be well suited to similar reservoirs and fields in the future.
A New Laboratory Technique to Enhance Proppant Consolidation During Propped Hydraulic Fracturing Treatment
Authors: Alqam Mohammad Hassan , Al-Makrami Adnan Hussain , Abass Hazim Hussain
Published: January 2022

The objectives of this investigation were to perform a rock mechanical study to evaluate long term stability of Resin-Coated Proppant (RCP), combined with various additives currently being used in screenless propped hydraulic fracturing completions in the sandstone formations. Thereby providing a tool for the industry to know exactly the duration of the shut-in time before putting a well back onto production.

A new experimental method was developed to monitor the curing process of RCP as temperature increases. The velocity of both shear and compressional waves were being monitored as a function of temperature, while the tested RCP sample was being housed in a pressurized vessel. The pressurized vessel was subjected to a variable temperature profile to mimic the recovery of the reservoir temperature following a propped hydraulic fracturing treatment. The placed proppant should attain an optimum consolidation to minimize the potential for proppant flow back. The study has been performed on various types of RCP samples under a range of reservoir conditions. The role of closure stress, temperature, curing time and carrier fluids in attaining a maximum strength of RCP following a propped hydraulic fracturing treatment have been investigated. Also, the Unconfined Compressive Strength (UCS) of various types of RCP have been measured.

The testing methods currently practiced in the industry to qualify proppant for field applications are based on physical characterization of several parameters such as the specific gravity of proppant, absolute volume, solubility, roundness, sphericity and bulk density. The sieve analysis, compressive strength, and API crush testing are also measured and reported. The API Recommended Practices; API RP56, API RP58 and API RP60 are the main procedures used to test the suitability of proppants for hydraulic fracturing treatment. However, there is no published API testing method for RCP; therefore this study introduces a new testing procedure, using acoustic velocity as a function of temperature and compressive strength as a function of time; to qualify a given RCP for a particular reservoir of known stress and temperature.

The final outcome of this study is to establish a functional procedure for such measurements, in order to maximize the success of a propped hydraulic fracturing treatment and minimize the occurrence of flow back incidents.
Controlled Frac Height Growth Technique to Avoid Contacting Water-Bearing Layer
Authors: Al-Obaid Hashem , Asel Sultan A. , Hansen Jon , Wijaya Rio
Published: January 2022

Many techniques have been used to model, diagnose and detect fracture dimension and propagation during hydraulic fracturing. Diagnosing fracture dimension growth vs time is of paramount importance to reach the desired geometry to maximize hydrocarbon production potential and prevent contacting undesired fluid zones. The study presented here describes a technique implemented to control vertical fracture growth in a tight sandstone formation being stimulated near a water zone. This gas well was completed vertically as openhole with Multi- Stage Fracturing (MSF).

Pre-Fracturing diagnostic tests in combination with high-resolution temperature logs provided evidence of vertical fracture height growth downward toward water zone. Pre-fracturing flowback indicated water presence that was confirmed by lab test. Several actions were taken to mitigate fracture vertical growth during the placement of main treatment. An artificial barrier with proppant was placed in the lower zone of the reservoir before main fracturing execution. The rate and viscosity of fracturing fluids were also adjusted to control the net pressure aiming to enhance fracture length into the reservoir.

The redesigned proppant fracturing job was placed into the formation as planned. Production results showed the effectiveness of the artificial lower barrier placed to prevent fracture vertical growth down into the water zone. Noise log consists of Sonic Noise Log (SNL) and High Precision Temperature (HPT) was performed. The log analysis indicated that two major fractures were initiated away from water-bearing zone with minimum water production. Additionally, in- situ minimum stress profile indicated no enough contrast between layers to help confine fracture into the targeted reservoir. Commercial gas production was achieved after applying this stimulation technique while keeping water production rate controlled within the desired range.

The approach described in this paper to optimize gas production in tight formation with nearby water contact during hydraulic fracturing treatments has been applied with a significant improvement in well production. This will serve as reference for future intervention under same challenging completion conditions.
A State-of-the-Art Approach to Unlock the Potential of Complex Sour Reservoirs in Southern Oman During Global Market Downturn
Authors: Sayapov Ernest , Molenaar Mathieu , Nunez Alvaro , Benchekor Ahmed , Hadhrami Abdullah , Smirnov Dmitry , Amin Khalil Mohamed , Ainouche Dalil
Published: January 2022

Recent years and especially the coronavirus pandemic have been very challenging for the oil industry, resulting in a significant reduction in investment, forcing companies to review budgets and search for more efficient and economical technologies to achieve the target level of hydrocarbon production and revenue generation.

In PDO, one of the most challenging fields is "AS", where extreme downhole conditions require a very well-engineered approach to become economical. This field has already seen some of the most advanced technology trials in PDO that are also covered in multiple SPE papers. Based on the new approaches and techniques that were successfully implemented on recently drilled wells, it was decided to review the older, previously fractured wells in the area and assess them for a refracturing opportunity.

The main challenge in this project was that these older wells were previously hydraulically fractured in multiple target intervals, therefore both zonal isolation and successful placement of the new fracs were becoming the major concerns. As the planned coverage by the new fractures was to ensure no bypassed pay, the only applicable technology on the market was a pinpoint fracturing process, whereby the targeted placement is achieved through limited entry perforations and focused energy of the injected fluid.

The subject pinpoint technology anticipates that the limited entry sandblasting perforation is created and then proppant laden fluid is pumped through a sandblasting nozzle which is part of either a coiled tubing (CT) or a jointed pipe (JP) Bottom Hole Assembly (BHA), and the backside (or the annulus of the injection path) is used to maintain the positive backpressure from the top. This technology allows for choosing a desirable order of target interval selection inside the well, unlike conventional plug and perf or a simplified multistage completion, where the treatments must be placed only in order from bottom to top. Another advantage of this approach is a faster frac cycle through the elimination of wellbore cleanout requirement.

Being a unique and first-ever application in the Middle East, using CT for placing frac treatments through a jetting nozzle demonstrates the full scale potential of this approach not only in conventional wells but also in complex, sour and High Pressure (HP) environments that are often found in the Sultanate of Oman and in the Middle East. This paper will cover the advantages and disadvantages, complexity and requirements, opportunities and lessons learnt in relation to this approach.
Power Maximization in Standalone Photovoltaic System: An Adaptive PSO Approach
Authors: MP Anbarasi , S Kanthalakshmi
Published: January 2022

A control strategy for power maximization which is an important mechanism to extract maximum power under changing environmental conditions using Adaptive Particle Swarm Optimization (APSO) is proposed in this paper. An Adaptive Inertia Weighting Factor (AIWF) is utilised in the velocity update equation of traditional PSO for the improvement in speed of convergence and precision in tracking Maximum Power Point (MPP) in standalone Photovoltaic system. Adaptation of weights based on the success rate of particles towards maximum power extraction is the most promising feature of AIWF. The inertia weight is kept constant in traditional PSO for the complete duration of optimization process. The MPPT in PV system poses a dynamic optimization problem and the proposed APSO approach paves way not only to track MPP under uniform irradiation conditions, but also to track MPP under non uniform irradiation conditions. Simulations are done in MATLAB/Simulink environment to verify the effectiveness of proposed technique in comparison with the existing PSO technique. With change in irradiation and temperature, the APSO technique is found to provide better results in terms of tracking speed and efficiency. Hardware utilizing dSPACE DS1104 controller board is developed in the laboratory to verify the effectiveness of APSO method in real time.
The Effects of Breathing Exercises and Inhaler Training in Patients with COPD on the Severity of Dyspnea and Life Quality: A Randomized Controlled Trial
Authors: CEYHAN YASEMİN , KARTIN PINAR TEKİNSOY
Published: January 2022

Background: Severe dyspnea and poor quality of life are common in chronic obstructive pulmonary disease (COPD). The most important reason for this situation is the wrong applications in inhaler treatment. In addition, inhaler treatments that supporting non-pharmacological methods increases the effectiveness of the drug. The aim of this study is to determine the effects of breathing exercises and inhaler training for chronic obstructive pulmonary disease patients on the severity of dyspnea and life quality.Methods: The research is a randomized controlled trial. A total of 67 with COPD who complied. The patients who were randomized in two groups that Intervention 1 group were given pursed lip breathing exercise and inhaler training and Intervention 2 group were given only inhaler training. A follow-up after 4 weeks was carried out in both groups. Patient outcomes in both groups were COPD assessment test (CAT), Modified medical research council (mMRC), and St. George's respiratory questionnaire scales (SGRQ). This study followed CONSORT checklist for randomized conrolled trials. In data analysis independent t, Mann-Whitney U, ANOVA, Wilcoxon analysis, and Pearson Chi-square tests were used.Results: Pursed lips exercise and inhaler drug use skills of patients in the both group increased (p<0.001). The median value of the CAT and mMRC scores are is statistically significant for both groups (p<0.005). The mean of life quality scores of patients in both groups decreased and this result was found statistically significant in all sub-dimensions and in the total scale score for two groups (p<0.001). Although the increase in the quality of life and the decrease in the severity of dyspnea of the patients in both groups were significant, the two groups were not superior to each other (p>0.05).Conclusions: As a result of the study, it was found that the skill of applying inhaler and life quality of the patients increased, the severity of dyspnea decreased. Supporting inhaler treatments with non-pharmacological methods can increase drug efficacy and quality of life. Trial Registration: Clinical Trial Registry registration number: NCT04739488. Registered on 21 Feb 2021.
Predictive Value of the PaO2/FIO2 Ratio for Mortality in Patients with Acute Respiratory Distress Syndrome: A Systematic Review and Meta-Analysis
Authors: Yoshimura Satoshi , Hashimoto Katsuhiko , Shono Yuji , Tamura Takahiro , Uchimido Ryo , Ando Koichi , Okamori Satoshi , Yoshida Takuo , Yoshitake Shigenori , Okada Yohei
Published: January 2022

Background PaO2 /FIO2 (P/F) ratio has been used to define the severity of acute respiratory distress syndrome (ARDS) despite the controversy of its clinical utility. This systematic review and meta-analysis (SRMA) aimed to obtain summary estimates of predictive performance of the P/F ratio for predicting mortality in ARDS patients. Methods We included a study wherein the study population comprised ARDS patients in any clinical setting. Medline and Cochrane Central Registry of Controlled Trials were searched for all English language articles. We performed a SRMA on the accuracy of diagnostic prognostic tests using QUADAS-2 tool to evaluate the risk of bias. To pool the results, we applied the bivariate model and obtained summary point estimates of sensitivity and specificity with 95% CIs. Results Twenty-eight trials and 38270 patients were included in the meta-analysis. Most of the study settings were in the intensive care units. The overall risk of bias was high. The pooled sensitivity of the P/F ratio in all included studies for a P/F ratio of 100 was 43.6% (95% CI, 36.9-50.5%) and the specificity was 71.1% (95% CI, 66.7-75.1%) and those for a P/F ratio of 200 were 83.2% (95% CI, 78.2-87.2%) and 26.2% (95% CI, 21.2-31.9%). Interpretation The P/F ratio had high sensitivity and moderate specificity at a P/F ratio of 200 and 100 respectively, which supports the use of the P/F ratio for screening ARDS patients who are at risk of deterioration.Trial registration: The study was registered in UMIN with registration number 64 000041058.
A Hybrid Space-Time Modelling Approach for Forecasting Monthly Temperature
Authors: KUMAR RAVI RANJAN , Sarkar Kader Ali , Dhakre Digvijay Singh , Bhattacharya Debasis
Published: January 2022

Spatio-temporal forecasting has various applications in climate, transportation, geo-statistics, sociology, economics and in many other fields of study. The modelling of temperature and it forecasting is a challenging task due to spatial dependency of time series data and nonlinear in nature. To address these challenges, in this study we proposed hybrid Space-Time Autoregressive Moving Average-Generalized Autoregressive Conditional Heteroscadicity (STARMA-GARCH) model in order to describe and identify the behaviour of monthly maximum temperature and temperature range in Bihar. At the modelling process of STARMA, spatial characteristics are incorporated into the model using a weight matrix based on great circle distance between the regions. The residuals from the fitted STARMA model have been tested by Brock, Dechert, and Scheinkman (BDS) and Autoregressive Conditional Heteroscadicity-Lagrange Multiplier (ARCH-LM) test for the behaviour of nonlinearity and ARCH effect respectively. The test results revealed that presence of both nonlinearity and ARCH effect. Hence GARCH modelling is necessary. Therefore, the hybrid STARMA-GARCH model is used to capture the dynamics of monthly maximum temperature and temperature range. The results of the proposed hybrid STARMA (1 1 , 0, 0)−GARCH (0, 1) model has better modelling efficiency and forecasting precision over STARMA (1 1 ,0, 0) model.
Delta Tocotrienol as a Supplement to FOLFOXIRI in First Line Treatment of Metastatic Colorectal Cancer. A Randomized, Double-blind, Placebo-controlled Phase II Study
Authors: Raunkilde Louise , Hansen Torben Frøstrup , Havelund Birgitte Mayland , Thomsen Caroline Brenner , Rafaelsen Søren Rafael , Lindebjerg Jan , Jensen Lars Henrik
Published: January 2022

Purpose Triplet chemotherapy might be more effective than doublet chemotherapy in metastatic colorectal cancer (mCRC), but it may also be marked by increased toxicity. To investigate whether δ-tocotrienol, a vitamin E analogue, with its possible neuroprotective and anti-inflammatory effects reduces the toxicity of triplet chemotherapy, we conducted a randomized, double-blind, placebo controlled trial in mCRC patients receiving first line 5-fluorouracil, oxaliplatin and irinotecan (FOLFOXIRI).Methods We randomly assigned 70 mCRC patients to FOLFOXIRI plus δ-tocotrienol or FOLFOXIRI plus placebo. FOLFOXIRI was given in eight cycles followed by four cycles of 5-fluorouracil. δ-tocotrienol 300 mg or placebo x 3 daily was added during chemotherapy and for a maximum of two years. The primary endpoint was time to hospitalization or death during treatment with chemotherapy.Results Median time to first hospitalization or death was 3.7 months in the placebo group (95% CI 1.93-not reached (NR)), and NR in the δ-tocotrienol group (95% CI 1.87-NR) with a hazard ratio (HR) of 0.70 (95% CI 0.36-1.36, p=0.29)). In the placebo group 24 patients (71%) had oxaliplatin dose reductions compared to 17 patients (47%) in the δ-tocotrienol group (p=0.047).Conclusion The addition of δ-tocotrienol to FOLFOXIRI did not significantly prolong the time to first hospitalization or death compared to FOLFOXIRI plus placebo. Toxicity was manageable and not statistically different between the two groups. There was a statistically significant difference in dose reductions of oxaliplatin pointing to a possible neuroprotective effect of δ-tocotrienol.Clinicaltrials.gov identifier NCT02705300. Date of registration March 10, 2016.
Gait Rehabilitation for Foot and Ankle Impairments in Early Rheumatoid Arthritis: a Feasibility Study of a New Gait Rehabilitation Programme (GREAT Strides)
Authors: Hendry Gordon , Bearne Lindsay , Foster Nadine E , Godfrey Emma L , Hider Samantha , Jolly Lisa , Mason Helen , McConnachie Alex , McInnes Iain B , Patience Aimie , Sackley Catherine , Sekhon Mandeep , Stanley Bethany , Leeden Marike Van Der , Williams Anita , Woodburn Jim , Steultjens Martijn
Published: January 2022

BackgroundFoot impairments in early rheumatoid arthritis are common and lead to progressive deterioration of lower limb function. A gait rehabilitation programme underpinned by psychological techniques to improve adherence, may preserve gait and lower limb function. This study evaluated the feasibility of a novel gait rehabilitation intervention (GREAT Strides) and a future trial. MethodsThis was a mixed methods feasibility study with embedded qualitative components. People with early (<2 years) rheumatoid arthritis (RA) and foot pain were eligible. Intervention acceptability was evaluated using a questionnaire. Adherence was evaluated using the Exercise Adherence Rating Scale (EARS). Safety was monitored using case report forms. Participants and therapists were interviewed to explore intervention acceptability. Deductive thematic analysis was applied using the Theoretical Framework of Acceptability. For fidelity, audio recordings of interventions sessions were assessed using the Motivational Interviewing Treatment Integrity (MITI) scale. Measurement properties of four candidate primary outcomes, rates of recruitment, attrition and data completeness were evaluated.Results35 participants (68.6% female) with median age (inter-quartile range [IQR]) 60.1 [49.4-68.4] years and disease duration 9.1 [4.0-16.2] months), were recruited and 23 (65.7%) completed 12-week follow-up. Intervention acceptability was excellent; 21/23 were confident that it could help and would recommend it; 22/23 indicated it made sense to them. Adherence was good, with a median [IQR] EARS score of 17/24 [12.5-22.5]. One serious adverse event that was unrelated to the study was reported. Twelve participants’ and 9 therapists’ interviews confirmed intervention acceptability, identified perceptions of benefit, but also highlighted some barriers to completion. Mean MITI scores for relational (4.38) and technical (4.19) aspects of motivational interviewing demonstrate good fidelity. The Foot Function Index disability subscale performed best in terms of theoretical consistency and was deemed most practical. ConclusionGREAT Strides was viewed as acceptable by patients and therapists, and we observed high intervention fidelity, good patient adherence and no safety concerns. A future trial to test the additional benefit of GREAT Strides to usual care will benefit from amended eligibility criteria, refinement of the intervention and strategies to ensure higher follow-up rates. The Foot Function Index disability subscale was identified as the primary outcome.Trial registrationISRCTN14277030
A Comparative Pharmacokinetic Study to Evaluate the Bioequivalence and Safety of A Humanized Recombinant Monoclonal Antibody Targeting Human Epidermal Growth Factor Receptor 2 (HER2) with the Reference Herceptin in Healthy Chinese Subjects
Authors: Ding Fangfang , Huang Jie , Feng Zeying , Kuang Yun , Yang Shuang , Xiang Yuxia , Zou Chan , YANG Guoping
Published: January 2022

Purpose:This study aimed to evaluate the safety, tolerability, pharmacokinetics and bioequivalence of a test humanized recombinant monoclonal antibody targeting Human Epidermal Growth Factor receptor 2 (HER-2) with the reference Herceptin®.Materials and methods:The trial consists of two parts (part I and part II). Part I was an open-label, sequential-cohort dose-escalation study, QLHER2 (test) was intravenous infusion at single doses escalating from 0.2 to 6 mg/kg (0.2, 1, 2, 4 and 6mg/kg) and Herceptin(reference) 4 mg/kg in 16 healthy subjects, to evaluated the safety, tolerability and pharmacokinetics of QLHER2. Part II was a randomized, double-blind, parallel-group study to evaluate the bioequivalence of QLHER2 and Herceptin in 60 subjects.Results:Following a 1.5-h intravenous infusion of single ascending doses of QLHER2 (1, 2, 4, or 6 mg/kg) In part I, Cmax and Tmax were 19.43-120.01 μg/mL and 68.91-157.87 h, respectively. AUC0-t and CL were 1.91-34.21 h*μg/mL and 0.54-0.12 ml/h/kg, indicating decreased clearance at higher doses, with a greater than proportional increase in the AUC0-t , and the t1/2 was 68.91-157.87 h. In part II, Plasma concentrations appeared to be comparable between QLHER2 and Herceptin over the 70-day sampling period and the QLHER2/Herceptin ratio of the Cmax and AUC0-t was 105.90% (90% CI: 95.69-117.26) and 95.79% (90% CI: 87.74-106.40%), respectively.Conclusion:The 90% CIs of the Cmax and AUC0-t for QLHER2/Herceptin ratio were within the range of 80.0-125.00% indicated that QLHER2 was bioequivalent to Herceptin. The results supported for further evaluation of QLHER2.Trial registration number: ChiCTR2000041577 and ChiCTR2100041802Date of registration: December 30,2020 and January 5, 2021
Seasonal Variation and Source Apportionment of Inorganic and Organic Components in PM2.5: Influence of Organic Markers Application on PMF Source Apportionment
Authors: Xue Qianqian , Tian Ying-Ze , Wei Yang , Song Danlin , Huang Fengxia , Tian Shanshan , Feng Yinchang
Published: January 2022

PM2.5 samples collected over a 1-year period in a Chinese megacity were analyzed for organic carbon (OC), elemental carbon (EC), water soluble ions, elements and organic markers such as polycyclic aromatic hydrocarbons (PAHs), hopanes, steranes, and n-alkanes. In order to study the applicability of organic markers in source apportionment, this study analyzes the relationship between organic and inorganic components, and four scenarios were implemented by incorporating different combinations of organic and inorganic tracers. A positive correlation of SO42− with 4 rings PAHs can prove that coal burning directly emits a portion of sulfate. A positive correlation of NO3− with 5-7 rings PAHs are found, implying collective impacts from the vehicle source. The concentrations of OC and EC positively correlate with the 5-7 rings PAHs and Cu and Zn, which proves that part of Cu and Zn comes from vehicle emissions. Five factors were identified by incorporating only conventional components, including secondary source (SS, 30%), urban fugitive dust (UFD, 14%), cement dust (CD, 4%), traffic source (TS, 19%) and coal combustion (CC, 14%). Six factors were identified by incorporating conventional components and PAHs, including SS (28%), UFD (15%), CD (4%), CC (13%), gasoline vehicles (GV, 12%) and diesel vehicles (DV, 10%). Eight factors were identified by incorporating conventional components, PAHs, hopanes, and n-alkanes, including SS (26%), UFD (17%), CD (3%), GV (14%), DV (8%), immature coal combustion (ICC, 5%), mature coal combustion (MCC, 10%) and biogenic source (BS, 1%).
Analysis of Exotic Parasitic Infection in a Large Group of Imported Dogs Following an Animal Welfare Investigation
Authors: Wright Ian , Whitfield Vanessa , Hanaghan Runa , Upjohn Melissa , Boyden Paula
Published: January 2022

Background: Increased dog relocation can cause dissemination of parasite and vector populations and this is being recognised in countries across Northern Europe, including the UK. Data regarding the prevalence of exotic infections entering the UK would be beneficial to vets to help assess pets entering the UK from abroad, and to help calculate risk of establishment of novel pathogens. One such group of dogs were seized as part of an RSPCA-led animal welfare investigation and blood tested for exotic pathogens. Methods: As part of the RSPCA investigation, 151 dogs were removed from the site. Blood tests were performed for Babesia. canis, Ehrlichia canis, Hepatozoon canis and Leishmania infantum by PCR, Br.canis by antibody serology and D.immitis by blood antigen. In addition to pathogen screening, a serology titre for Rabies was measured for each dog. A clinical examination was performed by a veterinary surgeon and clinical signs recorded. Clinical signs data were analysed by the Fisher-Freeman-Halton Exact TestResults: Overall, 24% of the dogs tested positive for an infection. Two dogs were positive for Br.canis antibodies and had no clinical signs indicative of infection. Leishmania infantum was identified in 10.5% of dogs with all but two cases being diagnosed in dogs whose microchip originated in Romania. Hepatozoon canis was identified in 9.6% of dogs, all of which had a Romanian microchip. Dirofilaria immitis was identified in 4.1% of dogs, B.canis in 2.3% of dogs and E.canis was only present in 1.5% of dogs tested. Only four dogs were found to have co-infections. No significant association was found between the pathogens detected and presenting clinical signs. Conclusions: This study demonstrates a range of exotic pathogens entering the UK including Br.canis and demonstrates the importance of screening imported dogs. The emphasis for early recognition of exotic pathogens in imported dogs has relied on screening based on relevant clinical signs and the country of origin. While these factors are useful, this study demonstrated no significant association between presenting clinical signs and the pathogens carried.
Local Recurrence After Endoscopic Submucosal Dissection of Early Gastric Cancer
Authors: Choi Cheol Woong , Kim Su Jin , Ryu Dae Gon , Kang Dae Hwan , Kim Hyung Wook , Park Su Bum , Nam Hyeong Seok , Lee Si Hak , Hwang Sun Hwi
Published: January 2022

Background: Endoscopic submucosal dissection (ESD) is considered the treatment of choice for early gastric cancer (EGC) with a negligible risk of lymph node metastasis. However, locally recurrent lesions on artificial ulcer scars are difficult to manage. Therefore, predicting the risk of local recurrence after ESD is important to manage and prevent the event. This study aimed to elucidate risk factors associated with local recurrence after ESD of EGC.Methods: Between November 2008 and February 2016, consecutive patients (n=641; mean age, 69.3±9.5 years; men, 77.2%) with EGC who underwent ESD at a single tertiary referral hospital were retrospectively analyzed to evaluate the incidence and factors associated with local recurrence. Local recurrence was defined as the development of neoplastic lesions at or adjacent to the site of the post-ESD scar.Results: En bloc and complete resection rates were 97.8% and 93.6%, respectively. The local recurrence rate after ESD was 3.1%. The mean follow-up period after ESD was 50.7±32.5 months. One case of gastric cancer-related death (0.15%) was noted, wherein the patient had refused additive surgical resection after ESD for EGC with lymphatic and deep submucosal invasion. Lesion size ≥15 mm, incomplete histologic resection, undifferentiated adenocarcinoma, scar, and absence of erythema of the surface were associated with a higher risk of local recurrence. Conclusions: Predicting local recurrence during regular endoscopic surveillance after ESD is important, especially in patients with a larger lesion size (≥15 mm), incomplete histologic resection, surface changes of scars, and no erythema of the surface.
Mutational Analysis of Compound Heterozygous Mutation P.q6x/p.h232r in Srd5a2 Causing 46,xy Disorder of Sex Development
Authors: Yang Hui , Li Liwei , Zhang Junhong , Li Qing , Qiao Li , Li Pengcheng , Cui Yi , Li Shujun , Hao Shirui , Wu Tongqian , Liu Lili , Yin Jianmin , Hu Pingsheng , Dou Xiaowei , Li Shuping
Published: January 2022

Background: Over 100 mutations in the SRD5A2 gene have been identified in subjects with 46,XY disorder of sex development (DSD). Exploration of SRD5A2 mutations and elucidation of the molecular mechanisms behind their effects should reveal the functions of the domains of the 5α-reductase 2 enzyme and identify the cause of 46,XY DSD. Previously, we reported a novel compound heterozygous p.Q6X/p.H232R mutation of the SRD5A2 gene in a case with 46,XY DSD. Whether the compound heterozygous p.Q6X/p.H232R mutation in this gene causes 46,XY DSD requires further exploration. Results: To clarify the cause of 46,XY DSD in the affected family focused on here, SRD5A2 sequencing was performed. Heterozygous p.H232R mutation was identified in the proband’s father, so we concluded that this mutation originated from the paternal side of the family and did not cause 46,XY DSD. Meanwhile, heterozygous p.Q6X mutation was identified in the proband’s mother, maternal uncle, and maternal grandfather, indicating that this mutation originated from maternal side of the family and did not cause 46,XY DSD. To clarify the effect of the p.H232R mutation in SRD5A2 on dihydrotestosterone (DHT) production, p.H232R mutant SRD5A2 plasmids were transfected into HEK293 cells. LC-MS indicated that DHT production decreased compared with that in cells transfected with wild-type SRD5A2.Conclusions: Our findings confirmed that the compound heterozygous p.Q6X/p.H232R mutation in the SRD5A2 gene is the cause of 46,XY DSD. p.H232R mutation reduced DHT production while attenuating the catalytic efficiency of the 5α-reductase 2 enzyme.
Detection of Road Traffic Anomalies Based on Computational Data Science
Authors: Raiyn Jamal
Published: January 2022

The development of 5G has enabled the autonomous vehicles (AVs) to have full control over all functions. The AV acts autonomously and collects travel data based on various smart devices and sensors, with the goal of enabling it to operate under its own power. However, the collected data is affected by several sources that degrade the forecasting accuracy. To manage large amounts of traffic data in different formats, a computational data science approach (CDS) is proposed. The computational data science scheme introduced to detect anomalies in traffic data that negatively affect traffic efficiency. The combination of data science and advanced artificial intelligence techniques, such as deep leaning provides higher degree of data anomalies detection which leads to reduce traffic congestion and vehicular queuing. The main contribution of the CDS approach is summarized in detection of the factors that caused data anomalies early to avoid long- term traffic congestions. Moreover, CDS indicated a promoting results in various road traffic scenarios.
Main Controlling Factors and Empirical Models for Total Mercury and Methylmercury Accumulation in Rice (Oryza Sativa L.)
Authors: Du Shuyang , Wang Xingxiang , Zhou Zhigao , Zhang Taolin , Kamran Muhammad , Ding Changfeng
Published: January 2022

It has been reported that rice consumption is the main mercury (Hg) exposure pathway for humans, and soil properties could significantly affect the methylation and accumulation process of Hg in soil-rice system. In this study, 19 paddy soil with various properties were spiked with exogenous Hg(II) at three concentration levels to conduct a pot experiment after a 60-days aging period. Stepwise multiple linear regression was applied to determine the controlling soil factors and develop prediction models for the methylmercury (MeHg) concentration in soil and total Hg (THg) and MeHg in brown rice. The results showed that THg in brown rice was positively correlated with THg in soil, while it had a negative correlation with soil pH and clay content. Soil organic matter (OM) promoted Hg methylation and inhibited the accumulation of MeHg in brown rice. Soil pH and clay content were negative factors when soil MeHg was used to predict MeHg in brown rice. THg and MeHg in brown rice could be well predicted by soil THg, pH and clay content. These results may provide a theoretical basis for safe production of rice in Hg contaminated paddy field.
Marked Changes in Biochar’s Ability to Directly Immobilize Cd in Soil: Implication for Biochar Remediation of Cd Contaminated Soil
Authors: Ren Xinhao , He Jiayi , Chen Qiao , He Fei , Wei Ting , Jia Honglei , Guo Junkang
Published: January 2022

To investigate the change in biochar’s ability to directly immobilize Cd in soil ，a successive wheat cultivation with experiment was conducted. Three biochar with different Cd adsorption mechanisms were added into soils and a mesh bag was used to separate the soil particles (> 1 μm) from biochar. The results showed that the ash contents and anionic contents (CO 3 2- and PO 4 3- ) of the biochar decreased with the cultivation time, while the oxygen-containing functional groups content and CEC of the biochar increased. Resultly, the Cd concentration on biochar decreased, highly decreased by 68.9% for WBC300, while unstable Cd species (acid soluble and reducible fraction of Cd) on biochar increased with successive cultivation, increasing from 3% to 17% for WBC300 in FS. Correspondingly, the ability of biochar to inhibit Cd accumulation in wheat decreased. The results of this study illustrated that the ability of biochar to directly immobilize Cd in soil is not permanent, it gradually decreases with aging in soil. The adsorption mechanism of Cd on biochar changed from precipitation to complexation and ion exchange processes could be the main reason.
Reconstructed Brain Like Neural Network R-KFDNN
Authors: rongrong zhu
Published: January 2022

Through the neural system damage and repair process of human brain, we can construct the complex deep learning and training of the repair process such as the damage of brain like high-dimensional flexible neural network system or the local loss of data, so as to prevent the dimensional disaster caused by the local loss of high-dimensional data. How to recover and extract feature information when the damaged neural system (flexible neural network) has amnesia or local loss of stored information. Information extraction generally exists in the distribution table of the generation sequence of the key group of the higher dimension or the lower dimension to find the core data stored in the brain. The generation sequence of key group exists in a hidden time tangent cluster. Brain like slice data processing runs on different levels, different dimensions, different tangent clusters and cotangent clusters. The key group in the brain can be regarded as the distribution table of memory fragments. Memory parsing has mirror reflection and is accompanied by the loss of local random data. In the compact compressed time tangent cluster, it freely switches to the high-dimensional information field, and the parsed key is buried in the information.
Flexible Material Formulations for 3D Printing of Porous Beds with Applications in Bioprocess Engineering
Authors: Dimartino Simone , Galindo-Rodriguez Giuseppe Rafael , Simon Ursula , Conti Mariachiara , Sarwar Sulaiman , Narayanan Selva Athi , Jiang Qihao , Christofi Nick
Published: January 2022

Background: 3D printing is revolutionizing many industrial sectors and has the potential to enhance also the biotechnology and bioprocessing fields. Here, we propose a new flexible material formulation to 3D print support matrices with complex, perfectly ordered morphology and with tuneable properties to suit a range of applications in bioprocess engineering. Findings: Supports for packed-bed operations were fabricated using functional monomers as the key ingredients, enabling matrices with bespoke chemistry such as charged groups, chemical moieties for further functionalization, and hydrophobic/hydrophilic groups. Other ingredients, e.g. crosslinkers and porogens, provide the opportunity to further tune the mechanical properties of the supports and the morphology of their porous network. Through this approach, we fabricated and demonstrated the operation of Schoen gyroid columns with I) positive and negative charges for ion-exchange chromatography, II) enzyme bioreactors with immobilized trypsin to catalyse hydrolysis, and III) bacterial biofilms bioreactors for fuel desulfurization. Conclusions: This study demonstrates a simple, cost-effective and flexible fabrication of customized 3D printed supports for different biotechnology and bioengineering applications.
Berberine and Evodiamine Synergically Exert Anti-colorectal Cancer Effeccts via P38/MAPK/MAPKAPK2/HSP27 Signaling Pathway
Authors: Chen Ningning , Jiang Yifang , Yang Yi , Zhao Ziyi , Xiao Chong , You Fengming , Yan Ran
Published: January 2022

Objective: Combinatorial natural products have high application potential for treatment of complex diseases owing to their synergistic effects and multi-targeting effect. However, studies have not explored the therapeutic effect and the synergetic mechanisms of action combinations of natural products. The present study aimed sought to evaluate the synergistic antitumor effects of a combination of Berberine and Evodiamine, and explore the drug effect on proliferation, migration, invasion of HCT116 and RKO human colorectal cancer cells. Results: The effect of berberine and evodiamine at a specific paired dose (BER30μM, EVO 0.8μM) was explored. A combination of berberine and evodiamine had no effect on activity and proliferation of HCT116 and RKO cells. The combination regulates the cell cycle of HCT116 and RKO cells at different cell phases. Berberine mainly blocked the cell cycle at G0/G1 phase, whereas evodiamine induced cell cycle arrest at G2/M phase. The results showed that the combined effect of berberine and evodiamine does not offset each other, but plays a synergistic role in regulation of colon cancer cell cycle. Western blot analysis showed that the combination of berberine and evodiamine regulated cell cycle by downregulating expression of cdc25c and upregulating expression of p21. The combination significantly inhibited cell migration and invasion by regulating EMT related proteins, upregulating expression of E-cadherin and downregulating expression of N-cadherin. The combination of berberine and evodiamine significantly inhibited phosphorylation of P38 MAPK in HCT116 and RKO cells, and further inhibited phosphorylation of the downstream MAPKAPK2 and HSP27, thus playing a synergistic anti-colon cancer role.Conclusion: Berberine and Evodiamine exhibit synergistic antitumor effects by suppressing cell proliferation, inducing cell cycle arrest and inhibiting EMT by modulating P38MAPK /MAPKAPK2/HSP27 pathway.Significance of the study: To illustrate the potential mechanism of formula-based combination of natural products, and explore the potential applications of the combination and possible antitumor therapeutic targets.
Identification of the Glucose Metabolism Biomarkers of NASH: Weighted Gene Co-Expression Network Analysis
Authors: Chen Xingyuan , Ye Zhanhui , Chen Kequan , Xu Jiahui , Ye Liangying , Luo Yufeng , Xu Yangzhi , Liu Shiming , Yang Hui
Published: January 2022

Background
The genetic mechanism of glucose metabolism has not been elucidated in nonalcoholic steatohepatitis (NASH), and many genes are took part in glucose metabolism of NASH. In this study, we used the weighted gene co-expression network analysis (WGCNA) to find the key genes associated with glucose metabolism;
Methods
Data sets GSE96971 and GSE89632 from Gene Expression Omnibus (GEO) were analyzed by WGCNA. We screened the Hub gene from the GSE96971 dataset, and the selected Hub genes were verified by GSE89632 dataset. We then analyzed the dataset using the Gene Ontology (GO) term enrichment and the Kyoto Encyclopedia of Genome (KEGG) path analysis. Expression levels of the hub genes are assessed by qPCR analysis. The function of hub genes was verified by Nile Red staining and relative glucose consumption detection;
Results
The hub genes are mannosidase beta like (MANBAL), myc proto-oncogene protein (MYC), caspase 4 (CASP4), CDK5 regulatory subunit associated protein 3 (CDK5RAP3) and ZFP36 ring finger protein (ZFP36) in the datasets GSE96971 and the GSE89632. Further, these genes are mainly involved in the integral component of membrane and plasma membrane, the PI3K-AKT signaling pathway and the olfactory transduction according to the GO and KEGG results. These hub genes were significantly up-regulated in the palmitic acid (PA) cell model and methionine-choline-deficient medium (MCD) cell model. After knocking out the hub genes in PA model and the MCD model of NASH, relative glucose consumption was increased and lipid deposition was reduced compared with the control group;
Conclusions
MANBAL, MYC, CASP4, CDK5RAP3 and ZFP36 are elevated and involved in the pathogenesis of NASH. Further research on these genes are warranted.
Large spontaneous HBV DNA fluctuations and potential usefulness of a single point measurement of combined HBV DNA and quantitative HBsAg for the exclusion of HBeAg negative chronic hepatitis B: A prospective Tunisian cohort study
Authors: Chtourou Amel , Gargouri Saba , Elleuch Emna , Fki-Berrajah Lamia , Fahmi  , Taktak Awatef , Mnif Khouloud , Kassis Mondher , Hammami Adnene , Jemaa Mounir Ben , KARRAY HELA
Published: January 2022

Background/Aims: We aimed to describe spontaneous short-term hepatitis B Virus (HBV) DNA level fluctuations and to assess the usefulness of quantitative HBsAg (qHBsAg) in Tunisian patients with HBeAg-negative chronic HBV infection.Patients and methods: We included 174 treatment-naïve patients with chronic HBeAg-negative HBV. A one-year prospective follow-up was carried out with serial determinations of HBV DNA, alanine aminotransferase levels and qHBsAg. Patients were classified into three groups: inactive carriers (G1), patients with HBeAg negative chronic hepatitis B (CHB) (G2) and patients with indeterminate state (G3). For this latter group, a liver biopsy was indicated.Results: Only genotype D was detected. During the follow-up, 21.6% and 19.5% of patients with low initial (<2000 IU/mL) and intermediate viral load (2000-20000 IU/mL), experienced a subsequent increase in their HBV DNA levels above 2000 and 20000 IU/mL, respectively. Significant variations of HBV DNA levels (≥0.5 log10 IU/mL) were observed in 61.1% of patients at 6 months-interval. Among the 174 patients, 89 (51.1%) belonged to G1, 33 (19%) to G2 and 52 (29.9%) to G3. Fourteen patients have undergone liver biopsy, among whom 7 (50%) showed moderate to severe liver disease. Combination of HBV DNA <2000 IU/mL and qHBsAg <832 IU/mL excluded CHB in 98.4% of cases.Conclusions: This study highlights the large short-term HBV DNA fluctuations in Tunisian patients with HBeAg negative chronic HBV of genotype D. HBV DNA < 2000 IU/mL along with qHBsAg < 832 IU/mL excluded CHB in 98.4% of cases. Significant proportion of patients with indeterminate state within genotype D would have HBeAg negative CHB.
Soft Biometrics and Deep Learning: Detecting Facial Soft Biometrics Features Using Ocular and Forehead Region for Masked Face Images
Authors: Banati Urja , Prakash Vamika , Verma Rashi , Srivast Smriti
Published: January 2022

Soft Biometrics is a growing field that has been known to improve the recognition system as witnessed in the past decade. When combined with hard biometrics like iris, gait, fingerprint recognition etc. it has been seen that the efficiency of the system increases many folds. With the Pandemic came the need to recognise faces covered with mask in an efficient way- soft biometrics proved to be an aid in this. While recent advances in computer vision have helped in the estimation of age and gender - the system could be improved by extending the scope and detecting quite a few other soft biometric attributes that helps us in identifying a person, including but not limited to - eyeglasses, hair type and color, mustache, eyebrows etc. In this paper we propose a system of identification that uses the ocular and forehead part of the face as modalities to train our models that uses transfer learning techniques to help in the detection of 12 soft biometric attributes (FFHQ dataset) and 25 soft biometric attributes (CelebA dataset) for masked faces. We compare the results with the unmasked faces in order to see the variation of efficiency using these data-sets Throughout the paper we have implemented 4 enhanced models namely - enhanced Alexnet ,enhanced Resnet50, enhanced MobilenetV2 and enhanced SqueezeNet. The enhanced models apply transfer learning to the normal models and aids in improving accuracy. In the end we compare the results and see how the accuracy varies according to the model used and whether the images are masked or unmasked. We conclude that for images containing facial masks - using enhanced MobileNet would give a splendid accuracy of 92.5% (for FFHQ dataset) and 87% (for CelebA dataset).