bluuebunny/crossref_metadata_2025 huggingface dataset:
Survival of 48866 cancer patients: results from Nantong area, China
Authors: Wang Gao-Ren , Xu Hong , Chen Hai-Zhen , Chen Yong-Sheng , Ni Zhuo-Jian , Fan Li-Yun , Zhang Ai-Hong , Xu Pei-Pei , Qian Yun , Cai Bo , Chen Jian-Guo
Published: August 2023

Objective

This study aimed to provide a realistic observation of survival by major site for 48,866 cancer patients treated at a tertiary cancer hospital in a rural area of China.

Methods

Patients with cancer registered between 2007 and 2017 in the Nantong rural area were followed up. The starting date for survival calculation was the date of the first diagnosis of cancer at the Nantong Tumor Hospital, and the closing date was December 31, 2020. Observed survival (OS) was analyzed according to ICD-10 site, sex, age, region, and hospitalization period using the life table method and compared using the Wilcoxon (Gehan) statistic.

Results

The overall 5-year OS rate was 40.48% for all 48,866 patients, 30.19% for males, and 51.90% for females. The top five cancer sites, accounting for 60.51% of the total cases, were the esophagus, lung, stomach, liver, and cervix, with 5-year OS rates of 33.72%, 18.64%, 32.10%, 19.04%, and 71.51%, respectively. The highest 5-year OS was observed in the thyroid (87.52%) and the lowest was in the pancreas (6.37%). Survival was significantly higher in younger patients than in older patients, with 5-year OSs of 69.26% and 19.84% in those aged 20-29 and 90-99 years, respectively. Five-year OSs improved significantly from 39.35% in 2007-2011 to 41.26% in 2012-2017.

Conclusion

Overall survival improved over the years, although the improvement at some sites was not significant. The observed survival varies from region to region, reflecting differences in the patterns of major sites, disparities in proportions of hospitalization, and demographic characteristics.
Mouse maternal odontogenic infection with Porphyromonas gingivalis induces cognitive decline in offspring
Authors: Ishida Eri , Furusho Hisako , Renn Ting-Yi , Shiba Fumie , Chang Hung-Ming , Oue Hiroshi , Terayama Ryuji , Ago Yukio , Tsuga Kazuhiro , Miyauchi Mutsumi
Published: August 2023

Introduction

Porphyromonas gingivalis (P. gingivalis), a major periodontal pathogen, causes intrauterine infection/inflammation. Offspring exposed to intrauterine infection/inflammation have an increased risk of neurological disorders, regardless of gestational age. However, the relationship between maternal periodontitis and offspring functional/histological changes in the brain has not yet been elucidated.

Methods

In this study, we used a gestational mouse model to investigate the effects of maternal odontogenic infection of P. gingivalis on offspring behavior and brain tissue.

Results

The step-through passive avoidance test showed that the latency of the acquisition trial was significantly shorter in the P. gingivalis group (p < 0.05), but no difference in spontaneous motor/exploratory parameters by open-field test. P. gingivalis was diffusely distributed throughout the brain, especially in the hippocampus. In the hippocampus and amygdala, the numbers of neuron cells and cyclic adenosine monophosphate response element binding protein-positive cells were significantly reduced (p < 0.05), whereas the number of ionized calcium binding adapter protein 1-positive microglia was significantly increased (p < 0.05). In the hippocampus, the number of glial fibrillary acidic protein-positive astrocytes was also significantly increased (p < 0.05).

Discussion

The offspring of P. gingivalis-infected mothers have reduced cognitive function. Neurodegeneration/neuroinflammation in the hippocampus and amygdala may be caused by P. gingivalis infection, which is maternally transmitted. The importance of eliminating maternal P. gingivalis-odontogenic infection before or during gestation in maintenance healthy brain function in offspring should be addressed in near future.
Protective effects of Scutellariae Radix Carbonisata-derived carbon dots on blood-heat and hemorrhage rats
Authors: Zhang Meiling , Cheng Jinjun , Luo Juan , Li Changxiang , Hou Tingting , Zhao Yan , Wang Yaoxian , Qu Huihua , Kong Hui
Published: August 2023

As the charcoal processing product of Scutellariae Radix (SR), SR Carbonisata (SRC) has been clinically used as a cooling blood and hemostatic agent for thousands of years. However, the underlying active ingredients and mechanism of SRC still remained unspecified. In this study, SRC derived carbon dots (SRC-CDs) were extracted and purified from the aqueous solution of SRC, followed by physicochemical property assessment by series of technologies. The cooling blood and hemostatic effects of SRC-CDs were further evaluated via a blood-heat and hemorrhage (BHH) rat model. Results showed that the diameters of obtained fluorescent SRC-CDs ranged from 5.0 nm to 10.0 nm and possessed functional group-rich surfaces. Additionally, the as-prepared SRC-CDs showed remarkable cooling blood and hemostasis effects in BHH model, mainly manifested by significant improvement of elevated rectal temperature, inflammatory cytokines (TNF-α, IL-6, and IL-1β) levels, as well as protein expressions of myD88 and NF-κB p65, abnormal coagulation parameters (elevated APTT and FIB), hemogram parameters (RBC, HGB, and HCT), and histopathological changes in lung and gastric tissues. This study, for the first time, demonstrated that SRC-CDs were the cooling blood and hemostatic active components of SRC, which could inhibit the release of inflammatory cytokines by regulating myD88/NF-κB signaling pathway, and activating the fibrin system and endogenous coagulation pathway. These results not only provide a new perspective for the study of active ingredients of carbonized herbs represented by SRC, but also lay an experimental foundation for the development of next-generation nanomedicines.
Chemotherapeutic drugs for soft tissue sarcomas: a review
Authors: Tian Zhichao , Yao Weitao
Published: August 2023

Despite the low incidence of soft tissue sarcomas (STSs), hundreds of thousands of new STS cases are diagnosed annually worldwide, and approximately half of them eventually progress to advanced stages. Currently, chemotherapy is the first-line treatment for advanced STSs. There are difficulties in selecting appropriate drugs for multiline chemotherapy, or for combination treatment of different STS histological subtypes. In this study, we first comprehensively reviewed the efficacy of various chemotherapeutic drugs in the treatment of STSs, and then described the current status of sensitive drugs for different STS subtypes. anthracyclines are the most important systemic treatment for advanced STSs. Ifosfamide, trabectedin, gemcitabine, taxanes, dacarbazine, and eribulin exhibit certain activities in STSs. Vinca alkaloid agents (vindesine, vinblastine, vinorelbine, vincristine) have important therapeutic effects in specific STS subtypes, such as rhabdomyosarcoma and Ewing sarcoma family tumors, whereas their activity in other subtypes is weak. Other chemotherapeutic drugs (methotrexate, cisplatin, etoposide, pemetrexed) have weak efficacy in STSs and are rarely used. It is necessary to select specific second- or above-line chemotherapeutic drugs depending on the histological subtype. This review aims to provide a reference for the selection of chemotherapeutic drugs for multi-line therapy for patients with advanced STSs who have an increasingly long survival.
Comparison of plant diversity-carbon storage relationships along altitudinal gradients in temperate forests and shrublands
Authors: Lu Shuaizhi , Zhang Dou , Wang Le , Dong Lei , Liu Changcheng , Hou Dongjie , Chen Guoping , Qiao Xianguo , Wang Yuyouting , Guo Ke
Published: August 2023

Understanding the mechanisms underlying the relationship between biodiversity and ecosystem function (BEF) is critical for the implementation of productive and resilient ecosystem management. However, the differences in BEF relationships along altitudinal gradients between forests and shrublands are poorly understood, impeding the ability to manage terrestrial ecosystems and promote their carbon sinks. Using data from 37962 trees of 115 temperate forest and 134 shrubland plots of Taihang Mountains Priority Reserve, we analyzed the effects of species diversity, structural diversity, climate factors and soil moisture on carbon storage along altitudinal gradients in temperate forests and shrublands. We found that: (1) Structural diversity, rather than species diversity, mainly promoted carbon storage in forests. While species diversity had greater positive effect on carbon storage in shrublands. (2) Mean annual temperature (MAT) had a direct negative effect on forest carbon storage, and indirectly affected forest carbon storage by inhibiting structural diversity. In contrast, MAT promoted shrubland carbon storage directly and indirectly through the positive mediating effect of species diversity. (3) Increasing altitudinal gradients enhanced the structural diversity-carbon relationship in forests, but weakened the species diversity-carbon relationship in shrublands. Niche and architectural complementarity and different life strategies of forests and shrubs mainly explain these findings. These differential characteristics are critical for our comprehensive understanding of the BEF relationship and could help guide the differentiated management of forests and shrublands in reaction to environmental changes.
Warming appears as the main risk of non-adaptedness for western Mediterranean relict fir forests under expected climate change scenarios
Authors: Méndez-Cea Belén , García-García Isabel , Linares Juan Carlos , Gallego Francisco Javier
Published: August 2023

Circum-Mediterranean firs are considered among the most drought-sensitive species to climate change. Understanding the genetic basis of trees’ adaptive capacity and intra-specific variability to drought avoidance is mandatory to define conservation measures, thus potentially preventing their extinction. We focus here on Abies pinsapo and Abies marocana, both relict tree species, endemic from south Spain and north Morocco, respectively. A total of 607 samples were collected from eight nuclei: six from Spanish fir and two from Moroccan fir. A genotyping by sequencing technique called double digestion restriction site-associated DNA sequencing (ddRAD-seq) was performed to obtain a genetic matrix based on single-nucleotide polymorphisms (SNPs). This matrix was utilized to study the genetic structure of A. pinsapo populations and to carry out selection signature studies. In order to understand how Spanish fir and Moroccan fir cope with climate change, genotype–environment associations (GEAs) were identified. Further, the vulnerability of these species to climate variations was estimated by the risk of non-adaptedness (RONA). The filtering of the de novo assembly of A. pinsapo provided 3,982 SNPs from 504 out of 509 trees sequenced. Principal component analysis (PCA) genetically separated Grazalema from the rest of the Spanish populations. However, FST values showed significant differences among the sampling points. We found 51 loci potentially under selection. Homolog sequences were found for some proteins related to abiotic stress response, such as dehydration-responsive element binding transcription factor, regulation of abscisic acid signaling, and methylation pathway. A total of 15 associations with 11 different loci were observed in the GEA studies, with the maximum temperature of the warmest month being the variable with the highest number of associated loci. This temperature sensitivity was also supported by the risk of non-adaptedness, which yielded a higher risk for both A. pinsapo and A. marocana under the high emission scenario (Representative Concentration Pathway (RCP) 8.5). This study sheds light on the response to climate change of these two endemic species.
Elevated UV photon fluxes minimally affected cannabinoid concentration in a high-CBD cultivar
Authors: Westmoreland F. Mitchell , Kusuma Paul , Bugbee Bruce
Published: August 2023

Ultraviolet photons (UV) can damage critical biochemical processes. Plants synthesize photo-protective pigments that absorb UV to minimize damage. Cannabinoids absorb UV, so increased UV has the potential to increase cannabinoid synthesis. Studies in the 1980’s provided some evidence for this hypothesis in low-cannabinoid cultivars, but recent studies did not find an increase in cannabinoid synthesis with increasing UV in high-cannabinoid cultivars. These studies used low UV photon fluxes, so we examined the effect of higher UV photon fluxes. We used fluorescent UV lights with 55% UV-B (280 to 314 nm) and 45% UV-A (315 to 399 nm). Treatments began three weeks after the start of short days and continued for five weeks until harvest. Established weighting factors were used to calculate the daily biologically effective UV photon flux (UV-PFDBE; 280 to 399 nm). Daily UV-PFDBE levels were 0, 0.02, 0.05, and 0.11 mol m-2 d-1 with a background daily light integral (DLI) of 30 mol m-2 d-1. This provided a ratio of daily UV-PFDBE to DLI of 41 to 218% of summer sunlight in the field. Cannabinoid concentration was 3 to 13% higher than the control in UV treated plants, but the effect was not statistically significant. Fv/Fm and flower yield were reduced only in the highest UV treatment. These data support recent literature and lead us to conclude that an elevated flux of UV photons is not an effective approach to increase cannabinoid concentration in high-cannabinoid cultivars.
Drought and salinity stresses induced physio-biochemical changes in sugarcane: an overview of tolerance mechanism and mitigating approaches
Authors: Kumar Rajeev , Sagar Vidya , Verma Vivek Chandra , Kumari Mala , Gujjar Ranjit Singh , Goswami Sanjay K. , Kumar Jha Sudhir , Pandey Himanshu , Dubey Abhishek Kumar , Srivastava Sangeeta , Singh S. P. , Mall Ashutosh K. , Pathak Ashwini Dutt , Singh Hemlata , Jha Prakash Kumar , Prasad P. V. Vara
Published: August 2023

Sugarcane productivity is being hampered globally under changing environmental scenarios like drought and salinity. The highly complex nature of the plant responses against these stresses is determined by a variety of factors such as genotype, developmental phase of the plant, progression rate and stress, intensity, and duration. These factors influence plant responses and can determine whether mitigation approaches associated with acclimation are implemented. In this review, we attempt to summarize the effects of drought and salinity on sugarcane growth, specifically on the plant’s responses at various levels, viz., physiological, biochemical, and metabolic responses, to these stresses. Furthermore, mitigation strategies for dealing with these stresses have been discussed. Despite sugarcane’s complex genomes, conventional breeding approaches can be utilized in conjunction with molecular breeding and omics technologies to develop drought- and salinity-tolerant cultivars. The significant role of plant growth-promoting bacteria in sustaining sugarcane productivity under drought and salinity cannot be overlooked.
Iron uptake of etioplasts is independent from photosynthesis but applies the reduction-based strategy
Authors: Sági-Kazár Máté , Sárvári Éva , Cseh Barnabás , Illés Levente , May Zoltán , Hegedűs Csaba , Barócsi Attila , Lenk Sándor , Solymosi Katalin , Solti Ádám
Published: August 2023

Introduction

Iron (Fe) is one of themost important cofactors in the photosynthetic apparatus, and its uptake by chloroplasts has also been associated with the operation of the photosynthetic electron transport chain during reduction-based plastidial Fe uptake. Therefore, plastidial Fe uptake was considered not to be operational in the absence of the photosynthetic activity. Nevertheless, Fe is also required for enzymatic functions unrelated to photosynthesis, highlighting the importance of Fe acquisition by non-photosynthetic plastids. Yet, it remains unclear how these plastids acquire Fe in the absence of photosynthetic function. Furthermore, plastids of etiolated tissues should already possess the ability to acquire Fe, since the biosynthesis of thylakoid membrane complexes requires a massive amount of readily available Fe. Thus, we aimed to investigate whether the reduction-based plastidial Fe uptake solely relies on the functioning photosynthetic apparatus.

Methods

In our combined structure, iron content and transcript amount analysis studies, we used Savoy cabbage plant as a model, which develops natural etiolation in the inner leaves of the heads due to the shading of the outer leaf layers.

Results

Foliar and plastidial Fe content of Savoy cabbage leaves decreased towards the inner leaf layers. The leaves of the innermost leaf layers proved to be etiolated, containing etioplasts that lacked the photosynthetic machinery and thus were photosynthetically inactive. However, we discovered that these etioplasts contained, and were able to take up, Fe. Although the relative transcript abundance of genes associated with plastidial Fe uptake and homeostasis decreased towards the inner leaf layers, both ferric chelate reductase FRO7 transcripts and activity were detected in the innermost leaf layer. Additionally, a significant NADP(H) pool and NAD(P)H dehydrogenase activity was detected in the etioplasts of the innermost leaf layer, indicating the presence of the reducing capacity that likely supports the reduction-based Fe uptake of etioplasts.

Discussion

Based on these findings, the reduction-based plastidial Fe acquisition should not be considered exclusively dependent on the photosynthetic functions.
Developing a tactical irrigation and nitrogen fertilizer management strategy for winter wheat through drip irrigation
Authors: Zain Muhammad , Si Zhuanyun , Ma Haijiao , Cheng Minghan , Khan Adam , Mehmood Faisal , Duan Aiwang , Sun Chengming
Published: August 2023

Introduction

Agricultural activities in the North China Plain are often challenged by inadequate irrigation and nutrient supply. Inadequate and improper resource utilization may impose negative impacts on agricultural sustainability. To counteract the negative impacts, a deeper understanding of the different resource management strategies is an essential prerequisite to assess the resource saving potential of crops.

Methods

We explored plausible adaptation strategies including drip irrigation lateral spacings of 40 and 80 cm (hereafter referred to as LS40 and LS80, respectively), irrigating winter wheat after soil water consumption of 20 and 35 mm (hereafter represented as IS20 and IS35, respectively), and nitrogen fertilization scheme of a) applying 50% nitrogen as a basal dose and 50% as a top-dressing dose (NS50:50), b) 25% nitrogen as a basal dose and 75% as a topdressing dose (NS25:75), and c) no nitrogen application as a basal dose and 100% application as a top-dressing dose (NS0:100).

Results and discussion

The consecutive 2 years (2017–2018 and 2018–2019) of field study results show that growing winter wheat under LS40 enhanced the water use efficiency (WUE), grain yield, 1,000-grain weight, and number of grains per spike by 15.04%, 6.95%, 5.67%, and 21.59% during the 2017–2018 season, respectively. Additionally, the corresponding values during the 2018–2019 season were 12.70%, 7.17%, 2.66%, and 19.25%, respectively. Irrigation scheduling of IS35 treatment improved all the growth-related and yield parameters of winter wheat. Further, treating 25% nitrogen as a basal dose and application of 75% as a top-dressing dose positively influenced the winter wheat yield. While NS0:100 increased the plant height, leaf area index (LAI), and aboveground biomass as compared to the other application strategies, but high nitrogen was observed in deeper soil layers. Regarding soil environment, the lowest soil moisture and nitrate nitrogen contents were observed in LS80 during both growing seasons. Overall, coupling the IS35 with NS25:75 under 40-cm lateral spacing is a suitable choice for sustainable winter wheat production in theNorth China Plain. The results of our study may be helpful in advancing the knowledge of the farmer community for winter wheat production. The findings can also aid in advancing new insights among scientists working on soil water and nitrogen distribution in drip irrigation for better productivity.
Dry-milled flour rice ‘Seolgaeng’ harbors a mutated fructose-6-phosphate 2-kinase/fructose-2,6-bisphosphatase2
Authors: Kang Ju-Won , Lee Sang-Kyu , Shim Su-Hyeon , Shin Dongjin , Cho Jun-Hyeon , Lee Ji-Yoon , Ko Jong-min , Ji Hyeonso , Park Hyang-Mi , Ahn Eok-Keun , Lee Jong-Hee , Jeon Jong-Seong
Published: August 2023

‘Seolgaeng’, an opaque-endosperm rice (Oryza sativa) mutant, is used to prepare high-quality dry-milled rice flour. The mutation causing its opaque-endosperm phenotype was unknown. Map-based cloning identified a missense mutation in the gene FRUCTOSE-6-PHOSPHATE 2-KINASE/FRUCTOSE-2,6-BISPHOSPHATASE 2 (OsF2KP2) in Seolgaeng. Transfer DNA insertion and clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated nuclease 9 (Cas9)-induced f2kp2 mutants exhibited opaque endosperm. Rice harbors another F2KP gene, OsF2KP1. CRISPR/Cas9-induced double mutants of OsF2KP1 and OsF2KP2 (f2kp-d) possessed more opaque endosperm compared to f2kp2 single mutants, whereas the endosperm of the f2kp1 single mutant was normal. Grain hardness and damaged starch content were significantly reduced in f2kp2 mutants compared to the wild type and f2kp1. Amylose content was lower than normal in f2kp2 mutants but not f2kp1. Grain hardness and amylose content were much lower in f2kp-d than in f2kp2. Starch polymerization analysis revealed altered amylopectin structure in f2kp2 and f2kp-d mutants. F2KP activity was lower in f2kp2 and much lower in the double mutants when compared to the wild types, but f2kp1 showed no significant difference. In coleoptiles, hypoxia induced OsF2KP2 expression but downregulated OsF2KP1. These results suggest that OsF2KP2 functions as the main F2KP isoform in endosperm experiencing hypoxia, but OsF2KP1 may partially compensate for the absence of OsF2KP2. We propose that F2KP has a crucial role in inorganic pyrophosphate-utilizing energy metabolism for starch biosynthesis in rice endosperm.
Enhancing drought resistance in Pogostemon cablin (Blanco) Benth. through overexpression of ACC deaminase gene using thin cell layer regeneration system
Authors: Warsi Zafar I. , Khatoon Kahkashan , Singh Pooja , Rahman Laiq Ur
Published: August 2023

Pogostemon cablin cultivation faces massive constraints because of its susceptability to drought stress that reduces patchouli propagation and oil yield. The present study has achieved an efficient and rapid direct regeneration system for the transgenic production of P. cablin using Agrobacterium-mediated genetic transformation. To establish an efficient regeneration protocol for fast in-vitro multiplication of patchouli plants, leaf, petiole, and transverse thin cell layer (tTCL) explants were used and inoculated on an MS medium supplemented with different combinations of phytohormones. A comparative study showed a maximum regeneration frequency of 93.30 ± 0.56% per explant was obtained from leaf segments on optimal MS medium fortified with 0.2mg/L BAP and 0.1mg/L NAA. Leaf and petiole explants took 25-35 days to regenerate while tTCL section showed regeneration in just 15-20 days on the same medium. Subsequently, productive genetic transformation protocol OD600 0.6, AS 200µM, 30mg/L kanamycin, and infection time 5 min. was standardized and best-suited explants were infected at optimum conditions from the Agrobacterium tumefaciens (LBA 4404) strain harboring ACC deaminase to generate transgenic P. cablin Benth. (CIM-Samarth) plants. The investigation suggested that the optimized protocol provides a maximum transformation frequency of 42 ± 1.9% in 15-20 days from tTCL. The transgenic plants were shifted to the greenhouse with a 52.0 ± 0.8% survival frequency. A molecular docking study confirmed significant binding affinity of ligand ACC with ACC deaminase at the catalytic site, and ligand interactions showed four H-bonds at the binding pocket with amino acids Cys-196, Val-198, Thr-199, and Gly-200 that validate gene relative expression in transgenic plants. Among all transgenic acclimatized greenhouse-grown patchouli plants, line PT4 showed improved drought resistance under severe water stress as its RWC was 71.7 ± 2.3% to 75.7 ± 2.1% which is greater than the RWC of the control plant, 58.30 ± 0.21%. Analysis of the other physiological indicators, H2O2, chlorophyll content, and ROS result support drought resistance ability. Our study concluded that the first report on P. cablin, tTCL direct regeneration, and standardized transformation protocol created a new opportunity for genetic manipulation to achieve drought-resistant patchouli plants for cultivation in all seasons at the commercial level.
Analyzing the impacts of cadmium alone and in co-existence with polypropylene microplastics on wheat growth
Authors: Han Zhiwei , Osman Raheel , Liu Yi , Wei Zhangdong , Wang Lin , Xu Ming
Published: August 2023

Heavy metals typically coexist with microplastics (MPs) in terrestrial ecosystems. Yet, little is known about how the co-existence of heavy metals and MPs affect crops. Therefore, this study aimed to evaluate the impact of cadmium (Cd; 40 mg/L) alone and its co-existence with polypropylene (PP)-MPs (50 and 100 µm) on seed germination, root and shoot growth, seedling dry weight (DW), and antioxidant enzyme activities of wheat. The study demonstrated that the germination rate of wheat did not vary significantly across treatment groups. Yet, the inhibitory impact on wheat seed germination was strengthened under the co-existence of Cd and PP-MPs, as the effect of a single treatment on seed germination was non-significant. The germination index and mean germination time of wheat seeds were not affected by single or combined toxicity of Cd and PP-MPs. In contrast, Cd and PP-MPs showed synergistic effects on germination energy. Wheat root and shoot length were impeded by Cd alone and in combination with PP-MPs treatments. The DW of wheat seedlings showed significant change across treatment groups until the third day, but on the seventh day, marginal differences were observed. For example, on third day, the DW of the Cd treatment group increased by 6.9% compared to CK, whereas the DW of the 100 µm PP-MPs+Cd treatment group decreased by 8.4% compared to CK. The co-occurrence of Cd and PP-MPs indicated that 50 μm PP-MPs+Cd had an antagonistic impact on wheat seedling growth, whereas 100 μm PP-MPs+Cd had a synergistic impact due to the larger size of PP-MPs. The antioxidant enzyme system of wheat seeds and seedlings increased under single Cd pollution, while the activities of superoxide dismutase, catalase, and peroxidase were decreased under combined pollution. Our study found that Cd adversely affects wheat germination and growth, while the co-existence of Cd and PP-MPs have antagonistic and synergistic effects depending on the size of the PP-MPs.
Elicitation enhances the production of friedelin and epifriedelanol in hairy root cultures of Cannabis sativa L.
Authors: Kobtrakul Khwanlada , Rani Dolly , Binalee Asma , Udomlarp Pattarapol , Srichai Tatiya , De-Eknamkul Wanchai , Vimolmangkang Sornkanok
Published: August 2023

Cannabis sativa L. (hemp) has a global distribution and social impact, and it is widely used as a medicinal plant, food ingredient, and textile fiber. Its roots have received less attention than other parts, especially the inflorescence, leaves, and shoots. Triterpenoids, including friedelin and epifriedelanol, have been found in hemp roots, and their anti-inflammatory effects have been reported. In this study, the potential enhancement of triterpenoid accumulation in the roots of C. sativa by elicitation was examined. Hairy roots were successfully established, and they contained 2.02-fold higher triterpenoid levels than natural roots. Furthermore, hairy roots treated with 75 μM salicylic acid had 1.95-fold higher friedelin levels (0.963 mg/g DW) and 1.4-fold higher epifriedelanol levels (0.685 mg/g DW) than untreated hairy roots. These results suggested that the elucidation of hairy root cultures using an optimized elicitor could represent an alternative strategy to produce the valuable triterpenoids friedelin and epifriedelanol.
Internet use predicts Chinese character spelling performance of junior high school students: multiple mediating roles of pinyin input proficiency and net-speak experience
Authors: Luo Rong , Peng Yifan , Chen Jingjun
Published: August 2023

To examine the complex relationship between Internet use experience and character spelling performance among Chinese junior high school students, the study explored the multiple mediating roles of Pinyin input proficiency and net-speak experience. A total of 447 Chinese junior high school students aged 12–15 years old completed the Internet Use Experience and Pinyin Input Proficiency Assessment, the Net-speak Experience Questionnaire and the Chinese Spelling Test. The results showed that: (1) All investigated variables were significantly correlated with each other, but there was no direct relationship between Internet use and Chinese spelling performance. (2) Pinyin input proficiency and net-speak experience play a chain mediating role in the relationship between Internet use and Chinese character spelling performance. Teens’ Internet use experience indirectly and positively predicted Chinese character spelling performance through the mediation of Pinyin input method use and net-speak experience. The implication of this study is that Chinese children should be guided to engage in Internet activities that require Pinyin typing and use net-speak creatively in order to promote the traditional Chinese character spelling skills when instructing teenagers to engage in Internet activities.
Virtual reality rehabilitation program on executive functions of children with specific learning disorders: a pilot study
Authors: Di Giusto Valentina , Purpura Giulia , Zorzi Carla Fulvia , Blonda Rosanna , Brazzoli Elena , Meriggi Paolo , Reina Tarjn , Rezzonico Silvia , Sala Roberta , Olivieri Ivana , Cavallini Anna
Published: August 2023

Background

The application of Virtual Reality (VR) in the field of rehabilitation has been widely studied, because it has already proven to be an effective intervention for a variety of physical and cognitive conditions. Nevertheless, its application in pediatric rehabilitation is more recent. This pilot study aims to examine whether a VR-rehabilitation program may have positive effects on the Executive Functions (EFs) of children with Specific Learning Disorders (SLD).

Materials and methods

Twenty-four children with diagnosis of SLD participated to the study (range 7–11 years) and performed the VR-training across 6 weeks in the CARE Lab, that was designed with appropriate structural measures and ad hoc fittings, to hide the sophisticated technology necessary to allow the child to experience a rehabilitative setting with recreational and semi-immersive features. Children were evaluated across three main time-points: T0, assessment of cognitive level and EFs immediately before the start of the intervention; T1, assessment of EFs immediately after the end of VR intervention; T2, follow-up of EFs after 6 months from the end of the VR intervention. The rehabilitation programs were customized according to clinical needs and/or single patient’s characteristics, proposing different games with variable complexity levels.

Results

Results showed that scores for visual attention, inhibition, flexibility, and planning abilities were significantly higher than before the intervention, and the most part of these ameliorations were maintained after 6 months.

Conclusion

These findings provide important inputs for the development of new innovative rehabilitation interventions for children with SLD that must be founded in ecological and evidence-based approaches.
Effects of multimorbidity patterns and socioeconomic status on catastrophic health expenditure of widowed older adults in China
Authors: Wang Zhen , Zeng Zhi
Published: August 2023

Background

The high multimorbidity and lower socioeconomic status (SES) of older adults, can lead to catastrophic health expenditures (CHEs) for older adults’ households. However, whether widowed older adults will bear such a financial burden has yet to be explored. The aim of this study was to investigate the influence of multimorbidity patterns and SES on CHE in Chinese widowed older adults.

Methods

Data was obtained from the 2018 China Health and Retirement Longitudinal Study (CHARLS). This is a cross-sectional study. A total of 1,721 widowed participants aged 60 years and older were enrolled in the study. Latent class analysis was performed based on 14 self-reported chronic diseases to identify multimorbidity patterns. The logistic model and Tobit model were used to analyze the influence of multimorbidity patterns and SES on the incidence and intensity of CHE, respectively.

Results

About 36.72% of widowed older adults generated CHE. The incidence and intensity of CHE were significantly higher in the cardiovascular class and multisystem class than in the minimal disease class in multimorbidity patterns (cardiovascular class, multisystem class, and minimal disease class). Among SES-related indicators (education, occupation and household per capita income), respondents with a middle school and above education level were more likely to generate CHE compared to those who were illiterate. Respondents who were in the unemployed group were more likely to generate CHE compared to agricultural workers. In addition, respondents aged 70–79 years old, geographically located in the east, having other medical insurance, or having fewer family members are more likely to generate CHE and have higher CHE intensity.

Conclusion

Widowed older adults are at high risk for CHE, especially those in the cardiovascular and multisystem disease classes, and those with low SES. Several mainstream health insurances do not provide significant relief. In addition, attention should be paid to the high-risk characteristics associated with CHE. It is necessary to carry out the popularization of chronic disease knowledge, improve the medical insurance system and medical service level, and provide more policy preferences and social support to widowed older adults.
Rescinding evidence-based care and practices during the initial COVID-19 outbreak in the United States: a qualitative study of the experiences of lactation support providers
Authors: Grady Julie , Mulpeter Ellie , Brimdyr Kajsa , Cadwell Karin
Published: August 2023

Background

The COVID-19 pandemic disrupted healthcare systems and services including along the childbearing continuum. The aim of this study was to explore the experiences and perceptions of professional lactation support providers who cared for breastfeeding families during the early months of the pandemic (March 2020 – August 2020) in the United States.

Design/methods

We conducted a qualitative survey among active lactation support providers in the United States. Eligible participants spoke English, were Certified Lactation Counselors who maintained an active certification and who provided lactation care and services prior to and after the onset of the COVID-19 pandemic. Participants were recruited via email from the national database of Certified Lactation Counselors obtained from the national certification body. All ten Health and Human Service regions of the United States were included. Demographic data was collected on each respondent. Qualitative survey responses were analyzed thematically following the framework method.

Findings

Six-hundred and seventy-four (674) Certified Lactation Counselors responded to the survey from June to July of 2022. Their responses fell within the overarching theme of rescinding evidence-based care and practices that had been in place prior to the pandemic. Affected care practices included the insertion of limits on access to care and insinuating stigma and bias based on COVID-19 status. Irregular appointment schedules and staffing shortages also affected care. Participants reported that separation of the mother and their infant became the norm. Decisions made by management seemed to be grounded in fear and uncertainty, rather than on the evidence-based principles that had been in place prior to the pandemic.

Conclusion

A lack of coordination, consistency and support, along with fear of the unknown, troubled lactation support providers and impacted their ability to provide evidence-based care and to maintain access to care for all families. The findings of the survey and analysis underscore the importance of adequately preparing for future public health crises by determining how evidence-based care and practices can be preserved in emergent situations.
The relationship between early childhood development and feeding practices during the dietary transitional period in rural China: a cross-sectional study
Authors: Liu Yihua , Chang Chun
Published: August 2023

Introduction

Early childhood feeding environments and practices not only affect children's nutritional status but also provide children with a variety of external stimulations to affect the development of the child's brain, especially for the first 1,000 days of children. The relationship between early childhood development (ECD) and feeding practices during the dietary transitional period has not previously been described.

Method

This study used quantitative survey data from the Integrated Early Childhood Development Project to investigate this association between ECD and feeding practices during the dietary transitional period in poor rural areas of China. Data concerning the child and family characteristics and feeding practices were collected through the questionnaire completed by caregivers. Developmental delays were explored through a five-pronged, structured, parent-completed Age and Stage Questionnaire. The chi-squared test and multivariate logistic regression analyses were used to explore the associated factors in ECD.

Results

The results showed that 33.6% of children had at least one area of developmental delay during the dietary transitional period. Of all five regions evaluated, the prevalence of fine motor developmental delays was highest (17.7%), followed by communication (14.9%), problem-solving (13.8%), personal-social skills (11.9%), and gross motor (11.8%), respectively. Significant predictors of increased odds of developmental delay included types of complementary foods (OR = 0.70, 95% CI = 0.53–0.94), adequate feeding frequency (OR = 0.69, 95% CI = 0.52–0.90), and breastfeeding time and bottle feeding (OR = 0.66, 95% CI = 0.50–0.88).

Discussion

According to the results, a high prevalence of developmental delay was observed in children during the dietary transitional period in the rural areas of China. The feeding practices of children were associated with their developmental status, including factors such as inadequate feeding frequency, types of complementary foods, breastfeeding duration, and low family income. These findings highlight the focus and potential direction for early identification and intervention.
Delay analysis of pulmonary tuberculosis in the eastern coastal county of China from 2010 to 2021: evidence from two surveillance systems
Authors: Liu Kui , Ge Rui , Luo Dan , Zheng Yan , Shen Zhenye , Chen Bin , Feng Wei , Wu Qionghai
Published: August 2023

Background

Tuberculosis (TB) remains a major public health challenge. However, indicators of delays in assessing effective TB prevention and control and its influencing factors have not been investigated in the eastern coastal county of China.

Methods

All notified pulmonary tuberculosis (PTB) cases in the Fenghua District, China were collected between 2010 and 2021 from the available TB information management system. Comparison of delays involving patient, health system, and total delays among local and migrant cases. Additionally, in correlation with available Basic Public Health Service Project system, we performed univariate and multivariate logistic regression analyses identified the influencing factors associated with patient and total delays in patients aged >60 years.

Results

In total, 3,442 PTB cases were notified, including 1,725 local and 1,717 migrant patients, with a male-to-female ratio of 2.13:1. Median patient and total delays of local TB patients were longer than those for migrant patients; the median health system delay did not show any significant difference. For patient delay among the older adult, female (cOR: 1.93, 95% CI: 1.07–3.48), educational level of elementary school and middle school (cOR: 0.23, 95% CI: 0.06–0.84) had a statistical difference from univariable analysis; however, patients without diabetes showed a higher delay for multiple-factor analysis (aOR: 2.12, 95% CI: 1.02–4.41). Furthermore, only the education level of elementary school and middle school presented a low total delay for both univariate (cOR: 0.22, 95% CI: 0.06–0.82) and multivariate analysis (aOR: 0.21, 95% CI: 0.05–0.83) in the older patients.

Conclusion

The delay of TB cases among migrants was lower than the local population in the Fenghua District, which may be related to the “healthy migrant effect”. It highlights that women, illiterate people, and people without diabetes are key groups for reducing delays among older adults. Health awareness should focus on these target populations, providing accessible health services, and reducing the time from symptom onset to diagnosis.
Altered synaptic organization in the mushroom bodies of honey bees exposed as foragers to the pesticide fipronil
Authors: Privitt James J. , Van Nest Byron N. , Fahrbach Susan E.
Published: August 2023

The pesticide fipronil is a GABA receptor antagonist that induces hyperexcitability in the insect nervous system. Its use is controversial because unintentional fipronil exposure may contribute to the ongoing global decline of pollinator populations. Sublethal doses of fipronil have been tentatively linked to reduced colony fitness and impaired learning in the western honey bee, Apis mellifera. The mushroom bodies, insect brain centers important for association learning and memory, contain fipronil-sensitive receptors and may therefore be directly affected by this pesticide. We investigated the synaptic organization of the mushroom bodies in worker honey bees exposed to fipronil using immunolabeling for a pre-synaptic marker and laser scanning confocal microscopy. Exposure of honey bee foragers to fipronil at a feasible field-realistic (1 ppb) concentration decreased the estimated density of immunolabeled microglomerular synaptic complexes in the lip and collar neuropil regions of the mushroom bodies. Effects were also evident after lower (0.1 ppb) and higher (4 ppb) exposures. Other indicators of synaptic organization (bouton number, surface area) were altered by exposure to fipronil in a dose-dependent fashion. These results indicate that sublethal doses of fipronil can result in atypical synaptic organization in the mushroom bodies of honey bees and suggest a mechanism by which fipronil, through perturbation of mushroom body-dependent functions, might diminish honey bee colony survival.
Shape of an obstacle affects the mediolateral trajectory of the lower limb during the crossing process
Authors: Miura Yuka , Yoshimoto Kohei , Shinya Masahiro
Published: August 2023

In previous studies involving obstacle crossing, vertical foot clearance has been used as an indicator of the risk of contact. Under normal circumstances, individuals do not always cross over obstacles with the same height on both sides, and depending on the shape of the obstacle, the risk of contact may differ depending on the foot elevation position. Therefore, we investigated whether task-related control of the mediolateral foot position is adapted to the shape of the obstacle. Sixteen healthy young adults performed a task in which they crossed over two obstacles with different shapes while walking: a trapezoidal obstacle and a rectangular obstacle, as viewed from the frontal plane. It was shown that when crossing over a trapezoidal obstacle, the participants maintained foot clearance by controlling the mediolateral direction, which chose the height that needed to be cleared. The results of this study suggest that the lower limb movements that occur during obstacle crossing are controlled not only in the vertical direction but also in the mediolateral direction by adjusting the foot trajectory to reduce the risk of contact. It was demonstrated that control was not only based on the height of the obstacle directly under the foot but also in the foot mediolateral direction, considering the shape of the entire obstacle, including the opposite limb.
Changes in friluftsliv (outdoor recreation) activities among Norwegian adolescents during the SARS-CoV-2 pandemic
Authors: Sæther Eivind , Mikalsen Stian , Lagestad Pål
Published: August 2023

Introduction

Friluftsliv (outdoor recreation) activities can provide both physical activity and experiences in nature, and improve quality of life. Regular physical activity is critical for young people's physical, social, and mental health. During the SARS-CoV-2 pandemic in 2020–2022, schools, gyms, and swimming pools were closed, and athletic teams and other physical activity services were prohibited from holding events. Despite these restrictions, access to nature and most friluftsliv persisted throughout the pandemic. The purpose of this study is to elucidate how the degree of activity in various friluftsliv activities changed during the pandemic, as well as identify the significance that friluftsliv had for the experience of community prior to and during the pandemic.

Methods

To achieve this goal, 287 young people aged 16–19 answered a questionnaire.

Results

The results showed that self-reported total friluftsliv decreased significantly by 12.1% during the pandemic compared to self-reported total friluftsliv activity in the year before the pandemic occurred. Modern friluftsliv experienced an overall activity decline in participation of 24.8%. Two of these activities had a significant decrease in activity level, while three of the activities had an unchanged level of activity. Traditional friluftsliv also experienced a significant decrease of 7.8%, with six of the seven friluftsliv activities exhibiting a significant decrease in activity level during the pandemic. Regarding the importance of friluftsliv for the experience of community, a substantial number of participants felt that friluftsliv activities had been more important during the pandemic than prior to the pandemic. In addition, many respondents indicated that the desire to be with friends, family, and girlfriends/boyfriends had become more important during the pandemic than before it.

Discussion

The findings related to the importance of friluftsliv activities to experiencing community were not unexpected. However, it was somewhat unanticipated that the two-year pandemic did not lead to more friluftsliv among young people, given the unavailability of other avenues of physical activity—which is concerning from a public health perspective, in terms of social, psychological, and physical health.