bluuebunny/crossref_metadata_2025 huggingface dataset:
El Ceibo (Ceiba sp.) como indicador de fuentes de contaminación atmosférica, en el Distrito de Chaclacayo, Lima, Perú
Authors: Blanco Obregón Jaziel Martín
Published: February 2025

The kapok (Ceiba sp.) Produces a fruit containing cotton-like fluff, which serves as a qualitative indicator of ambient air quality, due to the color that it acquires when exposed to the various particles in the environment.Kapok fluff was used, between the 2nd and 9th blocks of Nicolás Ayllón avenue in Chaclacayo, and complemented with samples from other areas of Lima.In 2008, the differences between the concentrations of lead in soil and cotton-like fluff indicated that this element comes mostly from the combustion motors of vehicles. Points with heavy traffic reported higher levels of Pb, not ruling out the presence of other particulates in the atmosphere.In 2016, the lower values in the fluff and soil, indicate that the reduction of lead as an additive in fuel affects the values of this mineral in the kapok fluff in Chaclacayo. The higher concentration of lead in the soil compared to the concentration in the kapok flufd indicates that the latter gets saturated with Pb.
Aplicación del modelo de colas al acarreo minero. Caso Mina Magistral
Authors: Ortiz Sánchez Oswaldo , Canchari Silverio Godelia
Published: February 2025

The study minimizes the cycle waiting time of loading and hauling equipment between loading points of the underground mining operation and unloading points on surface. For this purpose the existing loading and hauling system is first analyzed. Present system consists of four underground loading points and 4 unloading spots on surface. The waiting cost is estimated and then the increment in loading points underground is proved for reducing haulage cost which is a bearing component of the mining cost. By applying queuing techniques the possible reduction in waiting cost is obtained by increasing the number of loading points. The information gathered comes from loading and hauling times measured at site. Evaluated variables were number of loading points vs. waiting time cost of haulers and unit cost of underground ore and waste bins.
OCAS - Generando una Cultura de Seguridad
Authors: Rodríguez Portocarrero Pavel , Calderón Celis Julia Marilú , Berrocal Cerna Luis Antonio , Medina Escudero Ana María
Published: February 2025

This article is based on the foundations of the psychological trend called «behaviorism» and the preventive tool called safety based on behavior (SBB). Model behavioral observation applied to safety (BOAS) is a complement for the Management of Occupational Safety and Health, which aims to increase safe behavior of employees in an organization, looking at the short term generate workers attitude change (shown in their behavior) and long term by generating a safety culture that internalizes the worker and adopt as his own, bringing learning taught by the organization to all aspects of their daily lives. During the development of this article the implementation process, maturity and improvement of the Model BOAS is displayed. As the results show the gradual increase in safe behavior of workers in the organization.
Suelos contaminados con plomo en la Ciudad de La Oroya- Junín y su impacto en las aguas del Río Mantaro
Authors: Arce Siles , Calderón Marilú
Published: February 2025

This research is in response to the problem of pollution generated by the presence of the La Oroya smelter, whose activities resulted in lead contamination of air and soil in La Oroya for more than 90 years. For environmental and financial problems, Doe Run Peru Smelter current owner, has ceased operations since 2009, however persist environmental liabilities.As a result of the investigation it was found that the soils of the city of La Oroya Antigua this against the Metallurgical Complex is impacted with lead reaching as high values in some specific cases exceed 9000 mg / kg. surpassing all sense the Quality Standard Peruvian land for housing and commercial, industrial and extractive soil are 140 mg / kg and 1200 mg / kg. respectively.The lead content in the water of the river Mantaro reaches maximum values of 1 mg Pb / L in the adjacent slag deposit Huanchan area after heavy rainfall, indicating that there is impact of soil to the river , which it would be impossible life in the aquatic environment and the Water Quality Standard Peruano is 1 ug Pb / L. Due to the high dilution capacity of the river by its high flow, the contents of lead in the river down to near normal, however environmental damage occurs each time the rains are presented. La Oroya needs urgent soil remediation to be considered suitable for housing and for the river regains its aquatic life capacity, and / or is suitable for irrigation.From this research it is concluded that La Oroya needs urgent soil remediation to be considered suitable for housing, and that the river can once again find their capacity to aquatic life, and / or is suitable for irrigation.
Bodas del apu Suparaura: sexualidad y regeneración en la cosmología andina
Authors: Arroyo Aguilar Sabina , Suárez Rojas Luis Alberto , Rojas Zolezzi Enrique
Published: February 2025

En este artículo proponemos enfocar la importancia del hallazgo sobre el matrimonio del apu Suparaura con las jóvenes vírgenes como forma del culto a la fertilidad del mundo, donde la biósfera sexuada del entorno se convierte en un arquetipo de resignificación y de propiciación en los ciclos rituales de renovación cósmica, en un universo de competencias e intercambios recíprocos de los dioses andinos. Además, analizamos la importancia del apu Suparaura en cuanto a divinidad principal y montaña sagrada, según los mitos, representación de las wankas y la simbología del «toro encantado» de Supoqocha. No obstante, en el siglo XVI, Guamán Poma registró cómo una de las «wakas mayores» de Chinchaysuyo, recibiendo muchos sacrificios humanos y suntuosas ofrendas en su culto.
Efecto de los programas de actividades físico y físico-recreativas en la calidad de vida de personas adultas y adultas mayores
Authors: Obando-Piedra Adriana , Jiménez-Díaz Judith , Araya-Vargas Gerardo
Published: February 2025

El objetivo del presente estudio fue identificar el tamaño de efecto (TE) de los programas de actividades físico recreativas en la calidad de vida percibida por las personas adultas y adultas mayores y analizar posibles variables moderadoras, por medio de la técnica de metaanálisis. Treinta y dos estudios cumplieron con los criterios de elegibilidad establecidos a priori. La calidad de los estudios incluidos en el metaanálisis se evaluó utilizando la escala PEDro. Bajo el modelo de efectos aleatorios se calculó el TE de la diferencia de medias estandarizadas, calculando el impacto que generan las actividades físico recreativas en la percepción de la calidad de vida de las personas participantes. Se examinó la heterogeneidad (Q de Cochran) y la inconsistencia (I2) de los datos. Se encontró que participar en actividades físico recreativas mejora la percepción en la calidad de vida (TE=0.382, p<0.001., n=224), mientras que el grupo control no mejoró la percepción de la calidad de vida (TE=0.031, p=0.285, n=116). La edad, el sexo, la condición de salud, el nivel de actividad física, ni la cantidad de sesiones del programa influye en la percepción de la calidad de vida, mientras que la duración de las sesiones si influyen en la percepción de la calidad de vida.
Udział Kozaków w oblężeniu Starego Bychowa z 1702 roku
Authors: Kościelniak Karol
Published: February 2025

In 1702, one of the lesser-known I developed by historians sieges of Stary Bychow took place. This Sapiega fortress was blockaded by Lithuanian troops together with Zaporozhian Cossacks. The struggle, which lasted more than five weeks, ended successfully and Stary Bychow was taken by the Lithuanian army. The assistance of the Cossack troops of Hetman Ivan Mazepa contributed significantly to this victory. The article is structured in such a way as to provide an overview of the political situation, the state of the fortress fortifications, the forces of the two antagonists and, above all, the most accurate course of military action, including the participation and role played by the Zaporozhian Cossacks.
Oddziały kozackie i Kozacy w milicjach prywatnych w Rzeczypospolitej w XVIII w. Nowe spojrzenie
Authors: Ciesielski Tomasz
Published: February 2025

At the beginning of the 18th century, based on decisions made in 1699, the Cossack units, the so-called registered ones, which had earlier supported the state army in the defence of the south-eastern provinces of the Polish-Lithuanian Commonwealth for over 150 years, were liquidated. Those experiences related to numerous Cossack rebellions, the last in 1701–1703, meant that until 1792 it was not decided to recreate units recruited from Cossacks in the state army. However, the magnates and gentry were willing to use such units, by taking advantage of the applicable law, and they decided to maintain a court army. In the south-eastern provinces, having private militias was necessary in the face of raids by haidamaks (native bandits), Moldavian bandits, and Cossacks from the territories of Russia. Cossack units were easy to form due to a large recruitment base and cheap to maintain. The latter was due to granting farms exempt from all feudal services except compulsory police and military service. It is no wonder that Cossack units were an important, sometimes dominant element of the court armies of such large landowners as Chodkiewicz, Jabłonowski, Lubomirski, Potocki, or Radziwiłł (but with the reservation that only on their estates in Ukraine) presented in the article. The total number of court Cossack units was certainly much smaller than the 30,000 given by Kitowicz. However, they still constituted a significant group of combatants in the service of the magnates and the borderland nobility, protecting but also threatening their estates.
Андрей Геннадьевич Гуськов, Кирилл Александрович Кочегаров, Степан Михайлович Шамин, Русско-турецкая война 1686–1700 годов, Москва 2022, 528 ss., 17 il. (ООО «Русское Слово – учебник»)
Authors: Krokosz Paweł
Published: February 2025

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Geometrical quality inspection in 3D concrete printing using AI-assisted computer vision
Authors: Cui Weijiu , Liu Wenliang , Guo Ruyi , Wan Da , Yu Xiaona , Ding Luchuan , Tao Yaxin
Published: February 2025

3D concrete printing is an innovative technology poised to transform the construction industry by enabling the automated, layer-by-layer creation of structures directly from digital models. This approach offers numerous advantages over traditional construction methods, including reduced labor costs, faster build times, and the ability to produce complex geometries with high precision. However, unlike conventional mold-cast concrete, 3D printable concrete must support itself without external formwork, posing significant challenges related to material deformation during the printing process. Uncontrolled deformation can lead to structural instability, design deviations, and cumulative errors. Traditional methods for monitoring the geometrical quality of 3D-printed concrete are often insufficient in accuracy and efficiency. Recent advancements in artificial intelligence (AI) present new opportunities for addressing these challenges. AI-assisted methods leverage machine learning to analyze large datasets, enabling more accurate predictions and real-time monitoring and control of deformation during the 3D printing process. In this paper, we explored the application of AI-assisted methods for real-time deformation analysis in 3D concrete printing. Specifically, the Yolo-v5 algorithm, an AI-assisted object detection technique, was employed for the computer vision of extruded concrete filaments. Several quantitative metrics were proposed, including the layer height, layer angle, and curvature. In addition, the rheological properties of 3D-printed concrete were measured to refine the computer vision analysis results. Through experimental validation, we demonstrated the effectiveness of the developed AI-assisted computer vision system in monitoring the 3D concrete printing process.
The FOUR scale in intensive care and rehabilitation of patients with acute cerebral injury. The first results of a two-stage study
Authors: Arslanov O.Z. , Belkin A.A.
Published: February 2025

Background. Assessment of cerebral damage severity and prognosis is essential in choosing the treatment strategy for acute cerebral insufficiency. Correct analysis of injury and prediction of outcomes are obviously important for choosing the adequate treatment strategy. However, there is still no accurate objective assessment tool. Developed in 1974, the Glasgow Coma Scale (GCS) has become an integral part of clinical practice worldwide. Although GCS is a routine method, it has some significant drawbacks. The new Full Outline of UnResponsiveness (FOUR) coma scale is based on the minimum number of necessary tests to analyze severity of cerebral damage and prognosis. The FOUR scale is actively used abroad and In Russia. There is a Russian version of this scale approved and accepted by the FAR. Objective. To increase the effectiveness of clinical monitoring of patients with ACI by improving the discriminatory ability of the FOUR scale; to study sensitivity and specificity of this scale in chronic disorders of consciousness. Material and methods. A multiple-center non-randomized study included 2000 adults with ACI who were followed-up for 14 days in 10 ICUs. To test the hypothesis and study design, a single-center non-randomized cohort study was initiated using a continuous sampling method (“FOURACI” NCT06043167 ClinicalTrials.gov). Results. Spread of the FOUR scale and high need for effective clinical monitoring of patients with ACI inspired a two-stage observational prospective study in 2023. The first results when testing the hypothesis and study design confirm the advantages of the FOUR scale. Conclusion. The first results confirm high specificity of the FOUR scale for clinical assessment of impaired consciousness in intensive care patients with acute cerebral insufficiency.
Health seeking pathways for stroke survivors in a rural setting: Optimising early intervention for stroke rehabilitation in occupational therapy
Authors: Mkhize Phakeme Z.C , Phehlukwayo Stanford Mandlenkosi , Mpanza December Mandlenkosi
Published: February 2025

Background: Health seeking behaviour and health seeking pathways are interdependent concepts underpinning accessto healthcare. Understanding these concepts is critical for appropriate and time-dependant stroke interventions.Method: An explorative qualitative study design using one-on-one semi-structured interviews were conducted with16 participants who were either stroke survivors or caregivers of stroke survivors. The Socio-Ecological Model (SEM) wasused to guide data collection and the discussion of the findings in line with the objectives of this study.Findings: The traditional health practitioners (n=11) were the most preferred first encounter followed by public hospital(n = 2) and private hospital (n=1) while others (n=2) self-medicated. On average, it took 30 days for stroke survivors tonavigate the health system. The two themes emerging from this study, were 'health seeking delays', and 'the healthseeking behaviour factor'.Conclusions: A combination of factors influenced the health seeking delays at the levels of the SEM. The participants'health seeking behaviours were influenced by their knowledge, attitude, and beliefs at individual and interpersonallevels as well as dissatisfaction with health services at organizational and public policy levels of the SEM. In the SouthAfrican rural settings, stroke survivors seek medical help from multiple service providers prior to consulting the publichealthcare system due to diverse factors that affect them at interpersonal organizational, community and policy levels.Â Keywords: Health Seeking Behaviour, Health Seeking Pathways, Occupational Therapy Stroke InterventionsÂ
What’s new for the clinician – summaries of recently published papers (July 2024)
Authors: Yengopal V
Published: February 2025

Conventional techniques or digital computer-aided design and computer-aided manufacturing (CAD/CAM) by milling or three-dimensional (3D) printing are currently used for denture processing. CAD/CAM-milled discs are pre polymerised by the manufacturer under heat and pressure, which minimises their porosity and residual monomers; therefore, they exhibit improved mechanical properties and reduced bacterial adhesion. In contrast, resins for denture bases manufactured by 3D printing are polymerised by light, resulting in increased residual monomer concentrations and porosity. Hygiene and the disinfection of removable prostheses is essential to remove biofilm, increase longevity and maintain good oral health. Stomatitis has been reported to be the main cause of superinfection in patients with prostheses, especially infection with Candida spp. Poor denture hygiene has not only been associated with problems in the oral cavity but also with a 2.4 times higher risk of severe pneumonia.1 The ideal hygiene product or system would achieve an optimal level of disinfection of the dentures, be nontoxic and avoid alterations in the physical and mechanical properties, such as colour and dimension. In addition, it should be affordable. Among the mechanical methods for cleaning dentures, brushing is the most used because of its simplicity, effectiveness and low cost.
Lymphangioma: A Rare Intraoral Cystic Lesion
Authors: Odendaal Anneze , Kassan Ashwin , Afrogheh Amir
Published: February 2025

A 34-year-old female presented to an Oral and Maxillofacial surgeon with a complaint of a large painful cystic mass of her right upper lip. The patient mentioned that the lesion has been gradually increasing in size over the past few years. She also reported that the cystic mass was previously aspirated by an Oral and Maxillofacial surgeon who advised her to closely monitor the lesion. The patient had no medical history of note. She was taking Mypaid Forte tablets for pain relief and was using Gum Paroex 300 ml oral rinse twice daily. Extraoral examination was unremarkable. Intraoral examination revealed a 3 x 2 cm bluish submucosal cystic lesion of the right upper lip (Figure 1).
Lithofacies analysis of the Viola Limestone in South-central Kansas
Authors: Bornemann E. , Doveton J.H. , St. Clair P.N.
Published: February 2025

Core and cuttings studies of the Viola Limestone (Middle to Upper Ordovician) in south-central Kansas were integrated with numerical analyses of wireline logs in a composite evaluation of lithofacies variation. Two basic end-member facies were recognized; a low-porosity limestone of crinoidal packstones and grainstones, and a moderately porous cherty dolomitic limestone representing dolomitized wackestones and mudstones. These facies can be traced from both cuttings profiles and wireline log traces across the area as correlative subdivisions of the Viola. Fourier analysis of fine-scale variation of log-derived mineralogy suggests that facies migration was cyclic rather than episodic, in response to either an eustatic or tectonic control.
Lateral changes in facies, as interpreted from core cuttings, show a basic parallelism with the Central Kansas Arch. These range outwards from a somewhat restricted facies of sparsely fossiliferous wackestones and mudstones to crinoid packstones and grainstones representative of higher energy, open-marine environments. The influence of the Pratt Anticline as a positive feature contemporaneous with deposition is suggested in the upper Viola from core evidence and in the lower Viola from log analysis. In both instances, these appear as a relative enhancement of a calcite mud component on the structural axis, which graded outwards to less micritic higher energy facies on the flanks.
Computer processing of neutron, sonic, and density logs resulted in an interpretative lithofacies map in which the patterns reflect a complex history of deposition, diagenesis, and erosion. The disposition of limestone and dolomite facies as the dominant aspect of the Viola was dictated by erosional bevelling of the formation in Devonian and early Pennsylvanian times. On a regional scale, the Viola thins to the east with marked local variation controlled by differential fault-block movement. The lithofacies may also outline a region which represents solution weathering of the Viola to produce karstic carbonate zones and extensive deposits of residual chert. The map shows a distinctive grain which coincides with southerly continuations of fault trends associated with the Precambrian Central North American Rift System. These faults appear to have been intermittently active throughout the Paleozoic, influencing depositional trends, diagenetic patterns, erosional history, and the location of traps for Viola oil fields.
Subsurface structural maps over the Central North American rift system (CNARS), central Kansas, with discussion
Authors: Berendsen Pieter , Blair Kevin P.
Published: February 2025

Contour maps upon multiple horizons within a 125 by 60 mi (190 by 90 km) area of central Kansas reveal that the present-day structural configuration has resulted from repeated reactivation of Precambrian trends. Three major tectonic trends are defined. The Nemaha and Peace Creek tectonic zones identify the Paleozoic reactivated margins of the northeast-southwest-trending Keweenawan Central North American rift system (CNARS). The northwest-southeast-trending Fall River tectonic zone (FRITZ) parallels the Churchill accretionary terrane boundary. Structures were subjected to repeated movement during the Paleozoic, culminating with the Pennsylvanian Ouachita orogeny. Vertical tectonism involving a substantial component of strike-slip motion played an important role in the formation of observed structures.
Educational Conceptual Model Design Research Methodology
Authors: Tusquellas Natalia , López-Villanueva David , Palau Ramón , Santiago Raúl
Published: February 2025

This paper introduces Educational Conceptual Model Design a new research methodology to develop conceptual models in the field of educational technology. It is based on principles from Conceptual Design Methodology, Conceptual Modeling Methodology, Design-Based Research, and Design Science Research. It is a systematic methodology structured in four phases: problem definition, conceptual foundation, model development, and knowledge contribution. This methodology is designed to be streamlined and grounded in theory, focusing on consistency, replicability, and alignment with theoretical rigor. The methodology is particularly suited to respond to rapid advancements in technology and continuously evolving educational challenges, including the integration of emerging tools such as Artificial Intelligence in Education. Its main characteristic is that it offers a transition from problem identification to practical models with a special emphasis on the design process. This approach reduces the complexity of developing a model while maintaining its applicability across different educational technology areas, which can range from adaptive learning systems to data-driven learning analytics. The paper concludes by discussing the methodology’s contributions to theory and practice, its limitations, and potential future directions, including scalability, cross-disciplinary integration, and the incorporation of disruptive technologies. This methodology serves as a foundational framework for researchers and practitioners aiming to design innovative forward-thinking solutions that meet the dynamic needs of modern education.
ANTECEDENTES E CONSEQUENTES DO CONSUMO CONSPÍCUO EM EVENTOS DE RELEVÂNCIA SOCIAL PARA A AUTOEXPRESSÃO DO INDIVÍDUO
Authors: Moura Rubens Fernandes , Batista Ana Carolina Gonzalez
Published: February 2025

Os indivíduos aproveitam ocasiões especiais para selecionar bens de luxo (conspícuos) que projetem uma imagem de sucesso e prestígio, especialmente em eventos sociais onde é necessário que os demais membros reconheçam e validem essa identidade. Fazendo uso de um modelo de equações estruturais, este estudo propõe investigar os antecedentes e consequências do consumo conspícuo em eventos de importância social, considerando construtos de dimensões como centralidade do estilo de vida, consciência de marca, ostentação e sinalização, singularidade, conformidade social, consumo de status e autoexpressão. Os resultados destacam que a centralidade do estilo de vida influencia significativamente a consciência de marca, que, por sua vez, impacta dimensões centrais do consumo conspícuo. Além disso, o consumo de status desempenha um papel mediador na relação entre essas dimensões e a autoexpressão dos consumidores. Este modelo contribui para a literatura ao integrar fatores psicológicos e sociais no estudo do consumo conspícuo, oferecendo implicações teóricas e práticas para estratégias de marketing e a gestão de marcas.
Clinical-Biological Correlations of Chronic Respiratory Diseases and Oro-Dental System
Authors: D Munteanu , LI Pertea , A Vlase , R Ciorap , MI Ungureanu , CT Oniciuc
Published: February 2025

One of the most common chronic respiratory diseases, incapacitating the development and quality of life of patients and directly correlated with the oro-dental cavity is represented by asthma or Chronic Obstructive Pulmonary Disease (COPD), a chronic inflammatory disease affecting the airways, in which mast cells, eosinophils, and T lymphocytes, play an important role. A better understanding of the diagnosis and treatment of asthma became possible by accepting that the existence of chronic inflammation, with its variations, is reflected in the clinical condition of the patient, with implications on the dental status.
Scope of the study: Determination of the prevalence and severity of oro-dental pathology in patients with chronic respiratory diseases, respectively clinical and biological correlations, namely of individualized regimens, according to associated comorbidities that would contribute to improving the quality of life.
Materials and method: A study was conducted on a group of 167 patients hospitalized in the Internal Medicine Clinic, for one year (2021-2022) and also on 53 patients consulted in a dental ambulatory unit.
Results and discussion: The investigations showed an increased prevalence of gingivitis, chronic marginal superficial periodontitis, and coated tongue, possibly caused by insufficient ventilation in the nose or pulmonary obstructive diseases and mouth breathing predominance. Angular cheilitis and stomatitis have been also had in view, as diseases of the oral cavity, in patients with asthma and Chronically Obstructive Pulmonary Diseases (COPD). Such cases were more frequent in male than in female patients, a possible explanation being the higher prevalence of smoking and increased exposure to professional allergens for males, as well as a higher incidence of viral and / or bacterial superinfections.
Conclusion: The study carried out highlighted over 41.1% of the patients with chronic respiratory diseases and COPD/bronchial asthma (AB) presented angular cheilitis (11.6% cases), stomatitis (22.3% cases) and candidiasis (11.2% cases). In the experimental group, the level of oral hygiene is more satisfactory in women than in men, the same proportion being preserved in smoking and nonsmoking patients. Also, caries occurs more frequently in men than in women, the causes being, once again, health education, oral hygiene status and smoking.
SABER-FISH–Signal amplification for multiplexed fluorescence in situhybridization assays v1
Authors: Y. Kishi Jocelyn , J Beliveau Brian , K Saka Sinem , L Cepko Constance , Yin Peng
Published: February 2025

The SABER Technology The Signal Amplification By Exchange Reaction (SABER) method is used for amplifying signal from multiplexed in situ fluorescence staining experiments. Developed by the Yin and Cepko labs at Harvard University and the Wyss Institute, the technique uses Primer Exchange Reactions (PERs) to generate three-letter (A, T, C) concatemeric sequences in bulk in vitro reactions. These concatemers can then be in situ hybridized to fixed cells and tissues and act as scaffolds that localize fluorescent 'imager' strands. The method can further be paired with DNA-Exchange Imaging (DEI) to increase multiplexing via rapid stripping of old imager strands and hybridization of new imager strands (imager exchange) and/or cell segmentation with puncta counting on a per cell basis for quantitative analyses. See the following references and resources below for further information. SABER provides a scalable and cost-effective way to amplify multiplexed in situ stainings for RNA/DNA (SABER-FISH) and protein targets (Immuno-SABER, website, collection on protocols.io). SABER-FISH Multiplexed signal amplification enables rapid tissue mapping by increasing the number of targets that can be visualized per sample and reducing the exposure times required to see signals. Signal amplification in fluorescence in situ hybridization (FISH) assays can also increase sensitivity, potentially allowing smaller genomic and transcriptomic loci to be imaged, fewer probes to be applied, and/or less expensive microscopy setups to be utilized. SABER-FISH uses in vitro Primer Exchange Reactions (PERs) to synthesize long repetitive 'concatemer' sequences onto the 3' end of probes designed to be complementary to DNA and RNA targets of interest. After concatemer extension, probes are in situ hybridized to fixed cell and tissue samples, followed by a short secondary fluorescent hybridization that binds 20nt fluorophore-conjugated strands ('imagers') to the concatemers. Multiplexing with SABER-FISH is achieved through the use of orthogonal concatemer sequences appended to probe sets, which can be read out to spectrally separated fluorophores on complementary imagers. Imager sequences can also be stripped from one set of concatemers without disrupting the underlying probe binding, which allows new sets of imagers targeting different loci to be imaged in iteritave rounds of fluorescence imaging. This process of stripping and hybridization is referred to as DNA-Exchange Imaging (DEI). SABER-FISH signal can further be enhanced with a branching strategy, where multiple rounds of concatemerized probe binding create branched structures in situ. Below are a number of resources intended to help adoption of the technology, including references, animations, and protocols.
RNA Extraction From Mouse Tissue v1
Authors: Fan Yujie , Steele Chelsie
Published: February 2025

how to extract RNA from mouse tissue
Analysis of selected political Malaysiakini news visuals: A discursive news value and visual semiotics approach
Authors: Sazan Darwalis Binti , Md Yusoff Md Zahril Nizam Bin , Hj. Zaini Muhamad Fadzllah
Published: February 2025

This research examines the newsworthiness and ideological values embedded in political visuals, particularly those depicting leaders and politicians, as published by Malaysiakini, a leading Malaysian online news platform. Using a qualitative content analysis approach, the study is informed by Bednarek and Caple’s (2017) framework on discursive news values and Kress and van Leeuwen’s (1996) visual semiotics. It analyzed 12 political visuals published on Malaysiakini between April and October 2021. The analysis focuses on how these visuals align with three key newsworthiness values: eliteness, negativity, and positivity. The findings reveal that Malaysiakini often combines eliteness with either negativity or positivity, with eliteness serving as the dominant framing element for political figures. These visual representations reflect Malaysiakini’s editorial stance and ideological priorities, shaping how political leaders and issues are presented. Furthermore, the analysis highlights how political visuals function as ideological tools, embedding implicit meanings that align with the platform’s editorial identity and influencing audience perceptions. This study underscores the significant role visuals play in constructing and conveying ideologies. By portraying political figures and events in specific ways, Malaysiakini reinforces dominant political narratives, influencing public understanding of key issues. The research contributes to visual news analysis by demonstrating how media visuals can frame political discourse and shape ideological perspectives. Additionally, it offers insights into how Malaysian media organizations strategically use visual content to engage audiences and communicate editorial priorities.
Steps for developing the English Corpus of Public Administration (ECOPA) for public administration students: A qualitative corpus approach
Authors: Misnawati Misnawati , Atmowardoyo Haryanto , Sulaiman Iskandar , Bin Tahir Saidna Zulfiqar , Yusriadi Yusriadi
Published: February 2025

Corpus linguistics has opened up exciting possibilities for enriching classroom learning, yet only some corpus-based tools exist, specifically for didactic purposes. This is particularly true in public administration, where a lack of dedicated corpora presents a significant research opportunity. This study addresses this gap by developing the English Corpus of Public Administration (ECOPA), a novel corpus-based didactic tool designed to enhance the learning and teaching of public administration vocabulary. This study employed a qualitative approach grounded in corpus linguistics principles, utilizing the corpus analysis steps outlined by Toriida (2016) and a semantic scale validation process adapted from Dang (2020). ECOPA was meticulously compiled from 561 written text references representing diverse genres relevant to public administration, including narrative, general, academic, formal, and informal texts. Analysis of this corpus yielded a comprehensive list of 6.283 online words pertinent to the field. ECOPA is a valuable resource for public administration learners, as it provides exposure to authentic language use and facilitates vocabulary acquisition. Lecturers can utilize ECOPA to design engaging language-focused activities and assessments, while scholars can leverage the corpus for further research into the linguistic landscape of public administration. This study underscores the profound impact of corpus development on enriching linguistic resources within specialized fields.
On being heard: English, voice, and linguistic authority
Authors: Pennycook Alastair
Published: February 2025

This position paper explores the relationship between voice and different ways of understanding English. By emphasizing that English is dispersed, local, and variable, the World Englishes and English as a lingua franca (ELF) frameworks suggest that locally recognized varieties may be an avenue for diverse voices. This paper argues, however, that recognizing varieties of English does not go far enough (and indeed may be a regressive step) in opening a space to be heard. We need instead to think in terms of translingual practices (or Bahasa Gado-Gado) and alternative ways of framing language and voice. Viewing voice as the process of making oneself understood rather than as individual articulation presents a number of challenges for language education. Unless we consider the entanglements of English (the ways English is interwoven with the world), critical English pedagogies (addressing the inequalities between types of English), symbolic power (the challenge of being listened to), language assemblages (the dynamic gathering of different resources) and resourceful speakers (the capacity to align in language rather than adhere to language), our students may struggle to be heard.
Review and Analysis of Intelligent Methods of Critical Information Infrastructure Protection on the Example of the Financial Sector of the Russian Federation
Authors:   Financial University under the Government of the Russian Federation, Moscow, Russian Federation, Palchevsky E. V. , Antonov V. V. ,   Ufa University of Science and Technology, Ufa, 450008, Russian Federation
Published: February 2025

In recent years, cyberattacks, including DDoS attacks, on the critical information infrastructure of the Russian Federation have resulted in financial losses for companies, enterprises, individuals, universities and even hospitals. The damage reaches trillions of roubles, and on average, each large online shop that has been attacked can lose up to 600,000 roubles a day. And this is despite the fact that most companies have their own equipment and software to detect and filter DDoS attacks, or use the services of providers/data centres.
The main reason is that not all companies, providers and data centres have sufficient capacity to filter DDoS attacks of various types and types. In addition, an equally important reason is the misconfiguration of physical servers and network equipment ranging from switches to software-defined networks (SDNs)/content delivery networks (CDNs).
Thus, given the importance and necessity of ensuring the availability of critical information infrastructure in the era of digital economy, this paper presents a comprehensive systematic review of DDoS attack types and their intelligent filtering techniques.
The main findings and results of this study open up the possibility of implementing next-generation systems based on neural networks and computational clusters to analyse network traffic and detect DDoS attacks. In addition, these systems will help to solve existing critical problems, the main ones being the speed of response to emerging cyberattacks and the quality of filtering unauthorised network traffic.
On Neural Network Methods of Image Reconstruction and Super-Resolution
Authors:   National Research University "Moscow Power Engineering Institute", Moscow, Russian Federation, Rubinov K. A.
Published: February 2025

The methods for solving the problems of image inpainting and image super-resolution by means of image generation with neural networks are considered. Generative and adversarial neural networks are created and trained to solve them. It is shown that, in a wide range, the recovery quality almost does not depend on the fraction of damaged pixels, that adding residual blocks does not lead to its improvement, and that the generative adversarial network for resolution increase gives better results than the bicubic interpolation.
Gradient Reduction Algorithm for Determining Control Regulation: General Approach and Application to Chemical Kinetics Problems
Authors:   Ufa University of Science and Technology, Ufa, 450076, Russian Federation, Mustafina S. A. , Gallyamitdinov I. I. ,   Ufa University of Science and Technology, Ufa, 450076, Russian Federation
Published: February 2025

A gradient descent algorithm for optimal control of dynamic systems is developed taking into account the free right end of the trajectory and control constraints. A feature of the algorithm is the possibility of its generalization for various boundary conditions. The main attention is paid to the mathematical justification of the method and its application to problems of chemical kinetics. Numerical experiments are conducted confirming the efficiency of the algorithm for optimizing real chemical processes.
Develop a Web-Based Dynamic Assessment of Orthography Specificity as the Benchmark in Chinese Reading
Authors:   Faculty of Foreign Studies, Beijing Language and Culture University, Beijing, China, Guan Connie Qun
Published: February 2025

With increasing demands on online reading programs, it is necessary to develop an online assessment tool to monitor the word reading progress. We aimed to design such an online dynamic measure in Chinese based on the language-specific feature of orthographic representation to reveal the true relationship between reading and writing relationship. A cross-sectionally design from grade 3 to 5 in Chinese primary school students was conducted. Data were collected and analyzed using a mediation model to show that the online measure of lexical specificity and word reading were mediated by orthographic awareness with a developmental trend. The results suggested that orthographic specificity uniquely contributes to explaining word reading variance, with its impact more pronounced in lower graders. Our findings implied that the online computer-assisted dynamic assessment could be used to measure the children’s orthography for lower graders in the Chinese learning context. We established such a first lexical-specificity measure for online learning curves across grades. For future research, we should suggest a longitudinal follow-up should be adapted for static and dynamic performance of word reading in Chinese children.
Cultura Livre e Ciência Aberta: Implicações Freireanas na Educação a Distância
Authors: Coradini Fábio dos Santos , Matte Ana Cristina Fricke , Gomes Silvane Aparecida , Souto Tielle Alves
Published: February 2025

Hoje, a educação a distância se estabelece predominantemente em ambientes online, onde o software desempenha um papel crucial como suporte às atividades educacionais. Este artigo adota uma abordagem freiriana da EAD, fundamentada em análises da semiótica francesa, para examinar a presença do software na rede. Exploram-se as oposições entre livre e proprietário, aberto e fechado, bem como o conceito de liberdade. Partindo do pressuposto de que a escolha entre software livre ou proprietário, ciência aberta ou fechada, é indiferente para professores e alunos, dada a transparência no processo educacional, o artigo se destina ao professor não especialista em informática. Propõe uma reflexão sobre como os fundamentos e práticas do software livre, da cultura livre e da ciência aberta impactam a EAD. Destaca a importância dessa escolha para a coerência com a meta de formar identidades autônomas e sujeitos críticos.
 
Palavras-chave: Educação a distância. Software livre. Educação libertadora. Ciência aberta.
Integrating the TOE framework and DOI theory to dissect and understand the key elements of AI adoption in sociotechnical systems
Authors: Smit Danie , Eybers Sunet , Van der Merwe Alta , Wies Rene , Human Nica , Pielmeier Julia
Published: February 2025

This study is interested in increasing the likelihood of achieving the organisational adoption of artificial intelligence that has a positive  outcome. We argue that the technological-organisational-environmental framework provides a sound theoretical lens for analysing  how an organisation’s context influences the adoption and integration of artificial intelligence solutions. Furthermore, the diffusion of  innovation theory is proposed to identify enablers for transforming organisations. Together with the combination of technological-organisational-environmental and diffusion of innovation, the stages of diffusion are proposed as an evaluation paradigm to evaluate the effectiveness of the enabling factors. Furthermore, the elements and objectives of artificial intelligence adoption in the context of  data-driven organisations are included. From this, the research develops a comprehensive framework for studying the technical and  social AI adoption elements in an organisational environment where complex symbiotic relationships prevail. This study uses  generative artificial intelligence as a novel approach to exploring the framework’s usability. The evidence from our research indicates  that the developed framework can advance our comprehension of what drives the success or failure of artificial intelligence adoption  in organisations. Theoretically, it provides a tool for dissecting and understanding the key elements influencing this process.
The Convergence of Artificial Intelligence, Cloud Computing, and Edge Computing: Transforming the Tech Landscape
Authors: Manduva Vinay Chowdary
Published: February 2025

The convergence of Artificial Intelligence (AI), Cloud Computing, and Edge Computing is rapidly reshaping the technology landscape, creating innovative solutions across industries. This paper explores the synergistic relationship between these three technologies, highlighting their individual contributions and the transformative impact of their integration. AI, with its ability to analyze and make decisions from vast amounts of data, benefits from the computational power and scalability offered by Cloud Computing, while Edge Computing enables real-time data processing closer to the source, reducing latency and enhancing efficiency. Together, these technologies are driving advancements in fields such as healthcare, automotive, manufacturing, and smart cities, unlocking new business models, improving operational efficiency, and providing real-time decision-making capabilities. However, challenges related to data security, privacy, network reliability, and ethical considerations remain significant barriers to widespread adoption. Looking ahead, the convergence of AI, Cloud, and Edge is poised to continue evolving, influencing future technology trends and shaping the digital transformation across industries. This paper examines the current state of this convergence, its potential applications, and the implications for businesses and society.
The Strategic Evolution of Product Management: Adapting to a Rapidly Changing Market Landscape
Authors: Manduva Vinay Chowdary
Published: February 2025

From its status of a simple supportive operational function in today’s business world, product management has grown into a strategic value creating activity. These changes are due to technological developments, and changes in consumer behavior and increased market competition. In analyzing the article, it considers the primary driving forces of this change with emphasis on the approaches under which organizations are adopting innovation in the dynamic and unpredictable market environment. At the core of this transformation are data-driven approaches, Agile development methodologies and customers’ orientated methodologies, which have now directed the whole product process and the decision-making process of the product cycles.
Based on examples from best practice in this area, as well as bibliographic research, this article identifies innovation streams that are recalibrating this profession: AI, cross-functional teaming, and sustainability. It also examines issues that organizations encounter, including resistance to change, absence of or inadequate skills, and the dual issue of innovation and productivity.
The outcomes revealed reveal the need to focus on managing changes and volatility, and lifelong learning to achieve sustainable competitiveness. Realizing that the business environment is rapidly changing, organizations identify product management to successfully navigate it, while specifying the directions for developing technological potential in line with market demand. Herein, we provide practical recommendations and side notes for organizations and product managers who intend to thrive in this dynamic field.