bluuebunny/crossref_metadata_2025 huggingface dataset:
Toponimia minera en las concesiones de Alosno (Huelva): 1844-1860
Authors: Molina Díaz Francisco
Published: March 2021

En este trabajo se aborda el estudio de los topónimos impuestos a las concesiones mineras de Alosno, entre 1844 y 1860. Las características especiales de este tipo de toponimia la convierten en una fuente importante para el conocimiento de la psicología de los concesionarios y de la mentalidad del periodo. Se analizan los tipos de topónimos a partir de los nombres hallados en la documentación minera del Archivo Histórico Provincial de Huelva, atendiendo especialmente al motivo de imposición de los nombres. El rasgo más importante de este tipo de topónimos es la ruptura con las formas habituales en la toponimia general, ya que en esta destacan los nombres caracterizados por la relación entre el topónimo y los rasgos del terreno, pero en la toponimia minera destaca la arbitrariedad en la causa de imposición e incluso el uso de sustantivos abstractos que manifiestan la subjetividad y las expectativas del solicitante de la concesión minera, así como sus devociones particulares, las relaciones de amistad o parentesco y la percepción mitológica del lugar.
Penegakan Hukum dalam Perkara Tindak Pidana Penganiayaan Suporter Sepakbola di Kabupaten Bantu
Authors: Saputra Ridwan Tio
Published: March 2021

Penelitian ini dilatar belakangi oleh tindak pidana penganiayaan suporter sepakbola yang terjadi di Kabupaten Bantul. Tindak pidana penganiayaan terhadap suporter sendiri terjadi akibat dari terlalu fanatiknya suporter dalam mendukung tim kebanggaannya. Salah satu contoh adalah penganiayaan yang terjadi di Kabupaten Bantul tepatnya di Stadion Sultan Agung Bantul dala, pertandingan antara PSIM Yogyakarta VS PSS Sleman pada tanggal 26 Juli 2018.  Penelitian ini akan dijelaskan menggunakan metode penelitian normatif empiris dengan sumber data primer dan sumber data sekunder. Data primer diperoleh dari penelitian lapangan berupa wawancara dan kuisioner, sedangkan data sekunder bersumber dari kepustakaan, jurnal, skripsi, buku dan internet. Berdasarkan hasil penelitian, diketahui bahwa penegakan hukum terhadap pelaku tindak pidana penganiayaan suporter di Kabupaten Bantul pada kasus ini melanggar pasal 80 ayat (3) jo pasal 76C Undang-Undang Nomor 35 Tahun 2014 tentang Perubahan Atas Undang-Undang Nomor 23 Tahun 2002 tentang Perlindungan Anak. Ketiga terdakwa dikenakan sanksi pidana penjara masing-masing terdakwa I (Wahyu Timur Pribadi) dan terdakwa II (Lutfan Gian Firdaus) selama 3 (tahun) dan terdakwa III (Hawinta Akhsani Taqwim) selama 4 (empat) tahun dengan denda Rp. 100.000,000. (seratus juta rupiah).
Analysis of Motorcycle Parking Charges in the Klaten City Shopping Area
Authors: Ristuningsih Septiyanti , Hartarto Romi Bhakti
Published: March 2021

The implementation of regional autonomy adheres to the principle that financial sources originating from Regional Original Income (PAD) are more critical than sources outside PAD. Therefore, adequate financial sources are needed to finance the implementation of regional autonomy, one of which is through regional levies. One of the sources of revenue from sizeable regional user fees is parking fees. This sector is one of the essential sources of revenue because it always develops and develops an area (city). If it is appropriately managed, it can support PAD. This study aims to determine the number of potential parking fees in the shopping area of Klaten City and find strategies to optimize the potential for parking fees through surveys, observations, mathematical calculations, and comparative techniques to obtain an overview of the potential and conditions of the parking location. The results obtained include: 1) The potential for parking fees is currently Rp. 2,090,880,000.00 2) The efforts of local governments in increasing parking fees are not optimal 3) Strategies that can be used to increase parking fees can be done by establishing new regulations so that new potential the resulting amount will be IDR 2,471,040,000.00. Based on these findings, this research is expected to be a reference for the Klaten City government in optimizing the potential for parking fees; Besides, it is hoped that this research can enrich previous scientific findings related to parking fees.
DR. JOSÉ LUIS ARREDONDO GARCÍA (Semblanza)
Authors: Sánchez Márquez Pedro
Published: March 2021

Con estas palabras de Gabriel García Márquez inicio la semblanza de mi amigo y compañero José Luis Arredondo García deseando tener la vehemencia de poder transmitirles algunos de los aspectos más importantes que lo distinguieron en vida.Conocí a José Luis en enero de 1973 cuando iniciamos el Curso Propedéutico para la residencia de Pediatría Médica en el actual Instituto Nacional de Pediatría y, desde entonces, establecimos una relación tanto formativa como familiar, él entonces   ya casado con Erika, compañera de toda su vida y con dos pequeñas hijas. Desde entonces demostró capacidad de observación con actitud serena, reflexiva no provocativa, cualidades que le proporcionaron alta estima entre sus compañeros de generación y que fueron, entre otros, motivos para ser nombrado Jefe de Residentes al concluir la especialidad de Pediatría.
Lactancia materna exclusiva y factores maternos relacionados con su interrupción temprana en una comunidad rural de Ecuador
Authors: Flores Estrella María Daniela , Centeno Villavicencio María Laura , Maldonado Rivadeneira Juan-Carlos , Robles Rodríguez Julieta , Castro Burbano José
Published: March 2021

AntecedentesLa implementación de la lactancia materna exclusiva (LME) sigue siendo limitada en países en vías de desarrollo.ObjetivoEstimar en una parroquia rural del Ecuador la frecuencia de LME e identificar los factores relacionados con su abandono.Sujetos y métodos Estudio transversal en infantes (6 a 24 meses de edad), atendidos en el Centro de Salud de Conocoto (Pichincha - Ecuador). Se registraron datos maternos, estado nutricional de los lactantes, características de la lactancia materna y número de enfermedades agudas (diarreicas y respiratorias) previas.ResultadosDe los 155 lactantes (edad 12.6 ± 4.8 meses; 53.5% masculinos) estudiados, el 54.2% (IC95%: (46.3 – 61.8%) tuvieron un apego precoz a la lactancia y en el 86.5% (80.1% – 91.0%) esta fue a libre demanda. Solo el 49.6% (IC95%: 41.9% – 57.4%) tuvieron LME por seis meses y en el resto la duración fue de 2.7 ± 1.7 meses. Entre los grupos (con y sin LME) no hubo diferencias en su estado nutricional, pero sí en el número de episodios (≥6) de infecciones respiratorias (1.3% vs. 9.0%; p=0.03). Tres factores se relacionaron con un abandono de la LME: madre primípara (OR: 2.51; IC95%: 1.31 – 4.82; p<0.01), madre conviviente pero no casada (OR: 2.26; IC95%: 1.19 – 4.31; p=0.03) y madre que estudia o trabaja fuera del hogar (OR: 3.07; IC95%: 1.58 – 5.98; p<0.001).ConclusionesApenas la mitad de los niños tuvieron una LME. El abandono de la LME ocurre en los primeros tres meses, principalmente cuando las madres son primíparas, convivientes y laboran fuera del hogar. Es pertinente determinar la situación en otras parroquias rurales, aumentar la promoción de la LME involucrando a los padres y mejorar el seguimiento por parte del equipo de salud.
Parálisis Obstétrica del plexo braquial
Authors: Fraind Maya Gabriel , Loyo Soriano Luis Eduardo , Migoya Nuño Alfonso
Published: March 2021

La parálisis Obstétrica del plexo braquial (POPB) es una lesión por tracción del plexo braquial que genera parálisis flácida del miembro torácico, generalmente asociada a distocia de hombros y en raros casos en recién nacidos por cesárea. (1-4).La incidencia de la POPB varia entre 0.4-3 por cada 1000 recién nacidos, en los últimos años se ha visto una disminución debido a el entendimiento de la patología y el cuidado durante el trabajo de parto (1,3,5,6).El principal factor de riesgo para esta patología es la distocia del hombro el cual puede provocar tracción del plexo braquial (PB). Otros factores de riesgo asociados son: la utilización de fórceps, macrosomía, tiempo de parto prolongado, presentación pélvica, desproporción cefalopélvica, multiparidad y diabetes gestacional (1-3,6,7)Aunque en la literatura refiere que el 90% de los casos mejoran de forma espontánea (3 meses – 2 años), se estima que entre un 20-30% de los niños afectados quedan con un déficit residual (1,3-5)
Evaluation of Retinal Blood Flow in Glaucoma Patients Pre and Post Treatment using OCT angiography
Authors: H Riad , L Liu
Published: March 2021

Purpose: To evaluate retinal blood flow changes in glaucoma after intraocular pressure (IOP) lowering and to determine whether a correlation exists with retinal capillaries blood flow with IOP changes using optical coherence tomography angiography (OCTA)

Materials and Methods: OCTA images were collected from 24 glaucomatous eyes for the macula and 21 eyes for the optic disc in a prospective, cross-sectional observational study. Microvascular changes were analysed at two retinal layers: deep vascular plexus (DVP) and the superficial vascular plexus (SVP) as well as choriocapillaries (CC), were compared pre and six weeks post intraocular pressure (IOP) lowering treatment.

Results: Retinal blood flow across the macula showed no statistical significance at: SVP (z-score = -0.887), DVP (z-score = -1.093) and CC (z-score = -1.423). Further, no statistically significant difference in retinal blood flow was observed across the disc: SVP (z-score = -0.918), DVP (z-score = -1.057) and CC (z-score = -0.295). While the IOP showed improvement (z-score = -5.049), visual acuity deteriorated (z-score= 1.686)

Conclusion: No statistical difference was found in the data for all layers of both the areas surrounding the disc and macula at 6 weeks. OCTA can detect microstructural defects and future longitudinal studies may provide further insight into effects of IOP lowering treatment on retinal blood flow in glaucoma patients in longer term.
Complex evaluation of calendula officinalis flowers by main biologically active substances content
Authors: Malankina E. L. , Kozlovskaya L. N. , Biktimyrova L. V. , Komarova E. L.
Published: March 2021

Relevance. The demand for medicinal raw materials of Calendula officinalis L. is determined by the high content of pharmacologically significant compounds. Comparison of the most common ornamental cultivars of Calendula officinalis L.) in terms of yield and content of biologically active substances in the raw material helps to identify the most promising cultivars in terms of a set of indicators.

Materials and methods.The decorative cultivars Apricot, Art Shads, Fiesta Gitana, Kabluna golden-yellow, Orange King, Orange Balls, Yellow Gitana and Golden Balls, characterized by large inflorescences and terry, were used as prototypes. The cultivar Kalta, widely used for the production of medicinal raw materials, was chosen as a control. The experiments were laid on the experimental plots of the Fruit Growing Laboratory of the Russian State Agricultural University – Moscow Timiryazev Agricultural Academy. Productivity was determined as the total weight of inflorescences harvested during the entire season per unit area. The amount of flavonoids was determined by spectrophotometry. The amount of polysaccharides was determined according to the modified Draywood method. The lutein content was determined by HPLC.

Results.To obtain inflorescences we can recommend to use the variety Orange King, which, with a satisfactory yield (0.363 kg/m2), was characterized by a high content of flavonoids (1.2%) and satisfactory (35.9%) extractives and corresponds to the quality of raw materials required by GF XIV. In addition, this variety was characterized by an increased content of polysaccharides (20.3%). The Varieties Apricot and Yellow Gitana were characterized by high productivity (562±49 and 507±41 g/m2, respectively). Their raw materials can be used by production of herbal teas, where the content of flavonoids is not regulated. As sources of lutein, one should pay attention to the ligulate flowers of the varieties Apricot, Fiesta Gitana, Golden balls. In the variety Kabluna, with the maximum lutein content, the proportion of reed flowers in the inflorescence was only 38%. In varieties with large inflorescences, the content of lutein in reed flowers was higher (R=0.685). Large non-double inflorescences will be most productive in this indicator.
The Effectiveness of the Transaction Systems on the Dax Index
Authors: Trembiński Marek , Stawska Joanna
Published: March 2021

The purpose of the article/hypothesis: The aim of this article is to examine the effectiveness of trading systems built on the basis of technical analysis tools in 2015–2020 on the DAX stock exchange index. Efficiency is understood as generating positive rates of return, taking into account the risk incurred by the investor, as well as achieving better results than passive strategies. Presenting empirical evidence implying the value of technical analysis is a difficult task not only because of a huge number of instruments used on a daily basis, but also due to their almost unlimited possibility to modify parameters and often subjective evaluation.Methodology: The effectiveness of technical analysis tools was tested using selected investment strategies based on oscillators and indicators following the trend. All transactions were carried out on the Meta Trader 4 platform. The analyzed strategies were comprehensively assessed using the portfolio management quality measures, such as the Sharpe measure or the MAR ratio (Managed Account Ratio).Results of the research: The test results confirmed that the application of described investment strategies contributes to the achievement of effective results and, above all, protects the portfolio against a significant loss in the period of strong turmoil on the stock exchange. During the research period, only two strategies (Ichimoku and ETF- Exchange traded fund) would produce negative returns at the worst possible end of the investment. At the best moment, however, the „passive” investment achieved the lowest result. Looking at the final balance at the end of 2019, as many as four systems based on technical analysis were more effective than the „buy and hold” strategy, and at the end of the first quarter of 2020 – all of them. When analyzing the management quality measures, it turned out that taking into account the 21 quarters, the passive strategy had the lowest MAR index. The Sharpe’s measure is also relatively weak compared to the four leading strategies.
The Impact of Financial Leverage on A Company’s Market Valuation
Authors: Zimny Artur
Published: March 2021

The purpose of the article is to examine the impact of leverage on the market valuation of companies. The article verifies two hypotheses: 1. the degree of leverage is an important factor that impacts the market valuation of companies; 2. for companies with a high level of leverage, the impact of this leverage on their valuation is negative, and for companies with a low level of leverage, the impact is positive.The methodology of the study includes a critical literature review and empirical research based on correlation and regression analysis, including univariate and multivariate regression. The analysis covered quarterly data of ten energy companies listed on the Warsaw Stock Exchange. An important component of the research was classifying those companies into several groups, depending on their level of debt ratio in relation to the industry median debt ratio.The results of the research: The literature review did not provide an unequivocal conclusion to the problem. The empirical analysis did not give grounds to reject the first hypothesis; however, the second one was rejected. The research showed positive correlation and regression coefficients between the debt ratio and the price to book value ratio for highly leveraged companies and negative ones for companies with a low level of debt. The results are surprisingly contrary to the expectations based on theoretical premises.
Projekt oferty turystyki filmowej jako próba urozmaicenia polskiej oferty wyjazdowej do Irlandii w 2018 roku
Authors: Jarosz Agnieszka
Published: March 2021

Celem autorki artykułu jest zaproponowanie opartej na turystyce filmowej wycieczki tematycznej, która byłaby próbą urozmaicenia istniejącej oferty wyjazdowej do Irlandii. Artykuł mieści w sobie krótką klasyfikację definicji turystyki filmowej oraz przedstawia zarys jej rozwoju na świecie. Wyniki analizy ofert wyjazdów turystycznych do Irlandii w sezonie 2018 r. świadczą o tym, że Irlandia jest krajem chętnie odwiedzanym przez Polaków. Jednakże dobór miejsc pojawiających się w planach wycieczek w przypadku tej destynacji jest zbliżony do siebie w poszczególnych ofertach. Dostrzegając dużą popularność turystyki filmowej na świecie, w artykule zaprezentowano propozycję wycieczki tematycznej opierającej się na turystyce filmowej w Irlandii. Artykuł powstał na podstawie informacji zawartych w dostępnej literaturze naukowej, przewodnikach i na portalach internetowych.
Wody lecznicze jako podstawa rozwoju turystyki uzdrowiskowej (na przykładzie uzdrowiska Sopot)
Authors: Sywula Michaela
Published: March 2021

Stres i pośpiech, wszechobecne w życiu współczesnego człowieka, wpływają na zwiększenie zainteresowania pobytem w uzdrowiskach, dającym możliwość obcowania ze środowiskiem, w którym możliwe jest działanie wielu pozytywnych bodźców na organizm. Przedmiotem artykułu jest nadmorskie Uzdrowisko Sopot, słynące ze źródła wody mineralnej chlorkowosodowej, jodkowej i bromkowej, stosowanej w lecznictwie uzdrowiskowym. Do celów leczniczych (w tężniach, grzybkach inhalacyjnych oraz przy kuracji pitnej) wykorzystywana jest solanka o stężeniu 4,4%. Woda ta stanowi podstawę zabiegów leczniczych wykonywanych m.in. w Zakładzie Balneologicznym w Sopocie.
Using EMDR to Address Social Anxiety With Clients Who Stutter: Treatment Considerations
Authors: Huang Hsin-hsin , Pfuetze Mark
Published: March 2021

This article describes the treatment considerations when providing eye movement desensitization and reprocessing (EMDR) therapy to treat clients who stutter. Since stuttering is often developed in childhood and persists into adulthood, it has long-term impacts on the educational, social, psychological, and professional development of those who stutter. While stuttering can present with physiological impairments not amendable to psychological interventions, EMDR therapy may effectively decrease the psychological stressors (such as social anxiety and shame) that can intensify stuttering. The authors present an extensive literature review on the traumatic experiences and adverse effects of stuttering. They also discuss essential treatment guidelines when using EMDR to work with people who stutter (PWS), including processing developmental trauma when stuttering, experiences of being bullied because of stuttering, shame and internalized negative self-statements, distrust of one's body due to inability to control one's speaking, and the social anxiety and avoidance in dealing with triggering situations. The clinical instructions are illustrated with a case example of a 40-year-old college professor who experienced anxiety and shame related to persistent developmental stuttering, and who sought treatment due to difficulties speaking in front of his classes. After completing 20 sessions of EMDR therapy, the client reported decreased social anxiety and shame and was able to teach courses comfortably. Further research considerations using EMDR treatment with PWS are recommended.
Efficacy of EMDR Therapy on the Pain Intensity and Subjective Distress of Cancer Patients
Authors: Abdi Naeem , Malekzadeh Mohammad , Fereidouni Zhila , Behnammoghadam Mohammad , Zaj Parisa , Mozaffari Mohammad Amin , Rostaminejad Akbar , Salehi Zaker
Published: March 2021

The present study was carried out to investigate the efficacy of eye movement desensitization and reprocessing (EMDR) therapy in treating pain and subjective distress of patients with cancer. A randomized controlled trial was performed on patients with cancer suffering from moderate to severe cancer pain in Yasuj, Iran, in 2019 and 2020. Sixty patients aged 30–60 years who fulfilled the inclusion criteria were selected using a consensus sampling technique. Patients were randomly assigned to EMDR therapy or control groups based on random block allocation. EMDR therapy was administered in six to eight daily 1-hour sessions. The control group received the standard treatment provided by the hospital. A Numeric Pain-Rating Scale (NRS) and the Subjective Units of Disturbance Scale (SUDS) were used to assess pain and subjective distress before and after the intervention in each session. The collected data were analyzed by descriptive statistics, chi-square test, and independentttest using Statistical Package for the Social Sciences (SPSS) version 24. The mean pain intensity and subjective distress score in the experimental group before and after the EMDR intervention were significantly reduced (p< .001). In the control group, no decreases in NRS and SUDS scores occurred at any time (p> .05). Differences in pain scores between the groups were statistically significant (p< .001). EMDR can effectively and sustainably reduce the pain and subjective distress experienced by patients with cancer. Thus, EMDR is a recommended therapeutic option to mitigate pain and subjective distress among patients with cancer.
EMDR for Depression: A Meta-Analysis and Systematic Review
Authors: Sepehry Amir Ali , Lam Kerena , Sheppard Michael , Guirguis-Younger Manal , Maglio Asa-Sophia
Published: March 2021

The literature on the efficacy of eye movement desensitization and reprocessing (EMDR) for treating depression is heterogeneous due to research design, quality issues, and trials methodology. The current meta-analysis seeks to examine EMDR for depression with the aim of answering the aforementioned limitations. Thirty-nine studies were included for analysis after a review of the relevant literature. Univariate meta-regressions were run to examine dose-response and the effect of moderating variables. Subanalysis for primary and secondary depression showed a large, significant, and heterogeneous effect-size estimates, where EMDR significantly improved symptoms of depression in contrast to all control types. At post hoc, data were reexamined and a significant and large, yet heterogeneous, effect-size estimate emerged between the EMDR and control arm after the removal of two outliers [Hedges'g= 0.70, 95% CI =0.50–0.89,p-value < .01,I2= 70%,K= 37]. This is the first meta-analysis examining for the effect of EMDR comparing to various control modalities on depression with dose-response. We found (a) that studies were balanced at onset in terms of depression severity, and (b) a large and significant effect of EMDR on depression at the end of trials. Additionally, the significance of the aggregate effect-size estimate at the end of trials was unchanged by the intake of psychotropic medications, reported demographic variables, or EMDR methodology.
Exposing the Worst Forms of Child Labour in Bangladesh’s Leather Sector
Authors: Gorman Clare
Published: March 2021

As one of the country’s biggest employers and its second largest exporter, the leather sector is big business in Bangladesh. But it is also in crisis. A dramatic decrease in the global demand for leather since COVID-19 has led to the collapse of the supply chain with workers, especially children, bearing the worst of the brunt. As cracks in the industry’s surface widen, new research from the Child Labour: Action-Research-Innovation in South and South-Eastern Asia (CLARISSA) programme shines a light into its hidden corners, revealing examples of the worst forms of child labour (WFCL) at almost every turn.
LACK OF KNOWLEDGE ABOUT ORGAN DONATION AND TRANSPLANTATION AMONG DENTAL STUDENTS
Authors: Kusterer Liliane , Carvalho Caroline , Gallottini Mariana , Fernandes Karin , Bueno Patricia , Honório Heitor , Kubata Beatriz , Komesu Marilena , Santos Paulo
Published: March 2021

To assess the level of knowledge of Brazilian dental students about organ donation and transplantation. The 477 undergraduate Brazilian dental students, in the first or in the last year of the course, were questioned from March 2011 to June 2012. Data were analyzed by using descriptive statistics and differences between groups were analyzed by the chi-squared test. Only 84 (18%) reported having already attended to some class about the topic organ transplantation. Students reported knowing the brain death definition, but they did not know how the diagnosis of brain death is made (P =0.148). Students in the first and fifth years did not know about the maintenance care of potential organ donors (P=0.148) and how to approach the family about donation in case of brain death (P=0.561). Our study has demonstrated a lack of knowledge about organ donation and transplantation among undergraduate students in the four dental schools investigated. Nationwide, curriculum implementation is suggested in order to prepare dental care professions to integrate a transplantation team. Further investigation is necessary in order to evaluate dental student knowledge in other Brazilian regions.
Inclusion of People in Decision-Making
Authors: Tsekeris Charalambos , Demertzis Nicolas
Published: March 2021

Reflecting on the May 2019 European Parliament elections, the EU democratic ‎institutions are in need of efficiently responding to the discrepancies between‎ public agendas and policy-making (as shown in the Macedonia naming‎ dispute), and the threatening dynamics of authoritarian populism, as well as‎ to unpredictable reactions from diverse groups and citizens, especially from ‎the neglected, excluded and marginal ones. These citizens cannot handle complexity‎ and react by voting for protest candidates/movements and supporting ‎radical, yet oversimplified and inadequate, solutions to complex problems.‎ Given the potentiality of crisis cascades and that an over-standardized “one‎ size fits all” approach does not work anymore, the EU policy-making experts ‎should arguably turn their analytic attention to existing drivers of political ‎destabilisation by adopting new knowledge bases and sources. This pertains‎ to a fresh theoretical understanding of nonlinear sociopolitical phenomena‎(from populist reactions of any kind to social media behaviours), that is, a‎ deeper, complexity-friendly approach drawn from new scientific advancements‎ and coupled with innovative policy designs, aimed to rebalance the‎ system and to defend the European project against further failures.‎
Description of Lujinxingia Vulgaris Sp. Nov., Isolated From Coastal Sediment via Prey-traps
Authors: Wang Shuo , Mu Da-Shuai , Li Guang-Yu , Du Zong-Jun
Published: March 2021

Two Gram-stain-negative, facultatively anaerobic, oxidase-negative, catalase-positive bacilli, designated strain TMQ4T and TMQ2, were isolated from Xiaoshi Island in Weihai, PR China. The two strains had the abilities of predating other bacteria and were lured out with the prey Acinetobacter baumannii ATCC 19606T. Growth was observed within the ranges 25–45 oC (optimally at 37 oC), pH 6.5–9.0 (optimally at pH 7.5–8.0) and 1–8 % NaCl (optimally at 3–4 %, w/v). The draft genome sequences of strains TMQ4T and TMQ2 contained 184 contigs of 5,609,735 bp with a G+C content of 64.4 mol% and 148 contigs of 5,589,985 bp with a G+C content of 65.0 mol%, respectively. Phylogenetic analysis based on 16S rRNA gene sequences showed two strains belonged to the genus Lujinxingia, and their closest neighbour was Lujinxingia sediminis SEH01T, both with the similarity of 98.9 %. The phylogenomic topologies and analyses also demonstrated the taxonomic status of the two strains. Based on genomic analyses, the two strains lost biosynthesis pathway of several kinds of important chemical compounds and possessed the predatory indices of 2. Iso-C15:0 was contained in the predominant cellular fatty acids for strains TMQ4T and TMQ2. The major polar lipids of both strains consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and unidentified lipids, and the respiratory quinone was menaquinone MK-7 for both strains. The physiological, biochemical and phylogenetic properties suggest that two strains represent a novel species within the genus Lujinxingia. The name Lujinxingia vulgaris sp. nov. is proposed, with strain TMQ4T (= KCTC 62851T = MCCC 1H00392T) as type strain and strain TMQ2 (=KCTC 72079 = MCCC 1H00381) as reference strain.
Cardiac Events in Kawasaki Disease With Coronary Aneurysms Revisited, A Two-center Retrospective Cohort Study in Thailand
Authors: Santimahakullert Kanokvalee , Vijarnsorn Chodchanok , Wongswadiwat Yuttapong , Chanthong Prakul , Khrongsrattha Sappaya , Panamonta Manat , Chan-on Paradorn , Durongpisitkul Kritvikrom , Chungsomprasong Paweena , Kanjanauthai Supaluck , Soongswang Jarupim
Published: March 2021

Kawasaki disease (KD) is a common vasculitis in children, which may be complicated with coronary artery aneurysms (CAAs). We aimed to report the rates of major adverse cardiac events (MACE) and determine the risks of MACE in children diagnosed with KD and CAAs in Thailand. Data of 170 children diagnosed with KD and CAAs in two centers of Thailand between 1994 and 2019 was retrospectively reviewed. The risks of MACE were analyzed using multivariate analysis. Of 170 patients, forty-nine patients (28.8%) had giant CAAs. During the median time of follow-up (5.4 years; ranging from 22 days to 23 years), 19 patients (11.1%) experienced MACE including 12 coronary artery bypass grafting, 2 percutaneous coronary intervention and 5 patients with evidence of coronary occlusion. Coronary interventions were performed at 4 years (ranging from 0.01 to 9.5 years) after the KD diagnosis. Independent risks of MACE in KD with CAAs were from the presence of giant aneurysms (HR 16.55; 95% CI 2.52 to 108.63; p=0.003) and lack of intravenous immunoglobulin (IVIG) treatment (HR 11.43; 95% CI 2.8 to 46.62; p=0.001). The intervention-free rate at 5 and 10 years in patients with giant CAAs was 78.7% and 52.2%, respectively.Trial registration: TCTR20190125004
Production of Indole-3-Acetic Acid: A White Biotechnology for Weed Biocontrol
Authors: Bunsangiam Sakaoduoen , Thongpae Nutnaree , Limtong Savitree , Sri Nantana
Published: March 2021

Indole-3-acetic acid (IAA) is the most common plant hormone of the auxin class and regulates various plant growth processes. The present study investigated IAA production by the basidiomycetous yeast Rhodosporidiobolus fluvialis DMKU-CP293 using the one-factor-at-a-time (OFAT) method and response surface methodology (RSM). IAA production was optimized in shake-flask culture using a cost-effective medium containing 4.5% crude glycerol, 2% CSL and 0.55% feed-grade L-tryptophan. The optimized medium resulted in a 3.3-fold improvement in IAA production and a 3.6-fold reduction in cost compared with those obtained with a non-optimized medium. Production was then scaled up to a 15-L bioreactor and to a pilot-scale (100-L) bioreactor based on the constant impeller tip speed (Vtip) strategy. By doing so, IAA was successfully produced at a concentration of 3,569.32 mg/L at the pilot scale. To the best of our knowledge, this is the first report of pilot-scale IAA production by microorganisms. In addition, we evaluated the effect of crude IAA on weed growth. The results showed that weed (Cyperus rotundus L.) growth could be inhibited by 50 mg/L of crude IAA. IAA therefore has the potential to be developed as a herbicidal bioproduct to replace the chemical herbicides that have been banned in various countries, including Thailand.
Echocardiographic and Clinical Features in Diagnosis of Rare Cardiac Myxomas
Authors: yanhua qi , Ma miaoyan , Wei Yajuan , wu jinfang , gao peirong , li xiaopeng , liu baomin
Published: March 2021

Background: Cardiac myxoma(CM) is a rare cardiac disease, with the majority located in the left atrium and less commonly in the right atrium, right ventricular, biatrial, left ventricular, right ventricular outflow tract or heart valves. These rare CMs need to be distinguished from other masses. The purses of our study is to provide important value in finding the extra-atrial tumor and making the correct diagnosis by reviewed echocardiographic and clinical features of five rare CMs. Methods: We reviewed medical records and echocardiographic data in all patients with surgery diagnosis of rare CMs from January 2019 to June 2020 in the second affiliated hospital, Xi’an Jiaotong University. Results: Five patients were enrolled in our study. The sites of CMs were rare and located separately on RV, PV, LV, LA and LVOT, LA. Four cases was myxomas and one was lipoma. Five patients recovered well after operation. Conclusions: Cardiac tumors developed in unusual locations should be considered to exclude the possibility of myxoma. ﻿Subheadings: Echocardiographic of rare Cardiac Myxomas
Validity of the ACTS Intimate Partner Violence Screen in Antenatal Care: A Cross Sectional Study
Authors: Hegarty K , Spangaro J , Kyei-Onanjiri M , Valpied J , Walsh J , Chapman J , Koziol-McLain J
Published: March 2021

Background: Intimate partner violence (IPV) is a major public health problem with harmful consequences. In Australia, there is no national standard screening tool and screening practice is variable across states. The objectives of this study were to assess in the antenatal healthcare setting: i) the validity of a new IPV brief screening tool and ii) women’s preference for screening response format, screening frequency and comfort level.Methods: 1,067 antenatal patients in a major metropolitan Victorian hospital in Australia completed a paper-based, self-administered survey. The survey included four screening items about whether they were Afraid/Controlled/Threatened/ Slapped or physically hurt (ACTS) by a partner or ex-partner in the last 12 months; and the Composite Abuse Scale (reference standard). The ACTS screen was presented firstly with a binary yes/no response format and then with a five-point ordinal frequency format from ‘never’ (0) to ‘very frequently’ (4). The main outcome measures were test statistics of the four-item ACTS screening tool (sensitivity, specificity, predictive values, and area under the curve) against the reference standard and women’s screening preferences.Results: Twelve-month IPV prevalence varied depending on the ACTS response format with 8% (83) positive on ACTS yes/no format, 12.8% (133) positive on ACTS ordinal frequency format and 10.5% (108) on the reference Composite Abuse Scale. Overall, the ACTS screening tool demonstrated clinical utility for the ordinal frequency format (AUC, 0.80; 95% CI = 0.76 to 0.85) and the binary yes/no format (AUC, 0.74, 95% CI = 0.69 to 0.79). The frequency scale (66%) had greater sensitivity than the yes/no scale (51%). The positive and negative predictive values were 56% and 96% for the frequency scale and 68% and 95% for the yes/no scale. Specificity was high regardless of screening question response options. Half (53%) of the women categorised as abused preferred the yes/no scale. Around half of the women (48%, 472) thought health care providers should ask pregnant women about IPV at every visit.Conclusions: The four-item ACTS tool (using the frequency scale and a cut-off of one on any item) is recommended for written self-administered screening of women to identify those experiencing IPV to enable first-line response and follow-up.
Interactive Effects of Sediments and Urchins on Tropical Macroalgal Forests
Authors: Harris Rosalie , Wilson Shaun , Fulton Christopher
Published: March 2021

Macroalgal forests are a prominent component of tropical seascapes providing food and shelter for many species whilst subsidising secondary productivity in coastal ecosystems. Monitoring and managing macroalgae requires an understanding of key processes that alter these assemblages, especially at the local scale. Here we consider how sediment depth/cover and abundance of the macroalgal grazing urchin Tripneustes gratilla (Linnaeus, 1758), influence variation in community composition and physical structure of macroalgal patches within the World Heritage listed Ningaloo Reef, Western Australia. Our study found high urchin densities in sites with lower sediment loads and that sediments, urchin abundance and seascape position combined, explained 32% of the spatial variation in macroalgal forest community composition. We also found a greater occurrence of Sargassopsis, Caulerpa, Sirophysalis and Hormophysa in the lagoon where sediment cover/depth was high. Canopy height and cover of canopy forming macroalgae was also greater where sediment loads were high and urchin abundance low. Macroalgal genera with a strong and robust thallus were found in sediment depths up to 6 cm as opposed to those with softer, fragile thalli that were found in sediments < 2 cm. Our results demonstrate that high densities of urchins and mobile sediments can have profound effects on the composition and structure of tropical macroalgal forests. Changes to macroalgal forest community composition and physical structure will have flow-on effects for ecological processes such as productivity and habitat use which can alter the ecosystem goods and services macroalgal forests provide.
A Randomized Controlled Trial of Intranasal Oxytocin in Phelan-McDermid Syndrome
Authors: Fastman Jarrett , Foss-Feig Jennifer , Frank Yitzchak , Halpern Danielle , Harony-Nicolas Hala , Layton Christina , Sandin Sven , Siper Paige , Tang Lara , Trelles Pilar , Zweifach Jessica , Buxbaum Joseph D. , Kolevzon Alexander
Published: March 2021

BackgroundPhelan-McDermid syndrome (PMS) is a rare neurodevelopmental disorder caused by haploinsufficiency of the SHANK3 gene and characterized by global developmental delays, deficits in speech and motor function, and autism spectrum disorder (ASD). Monogenic causes of ASD such as PMS are well suited to investigations with novel therapeutics, as interventions can be targeted based on established genetic etiology. While preclinical studies have demonstrated that the neuropeptide oxytocin can reverse electrophysiological, attentional, and social recognition memory deficits in Shank3-deficient rats, there have been no trials in individuals with PMS. The purpose of this study is to assess the efficacy and safety of intranasal oxytocin as a treatment for the core symptoms of ASD in a cohort of children with PMS.MethodsEighteen children aged 5-17 with PMS were enrolled. Participants were randomized to receive intranasal oxytocin or placebo (intranasal saline) and underwent treatment during a 12-week double-blind, parallel group phase followed by a 12-week open-label extension phase during which all participants received intranasal oxytocin. Safety was monitored throughout the study period and efficacy was assessed using the primary outcome of the Aberrant Behavior Checklist – Social Withdrawal (ABC-SW) subscale as well as a number of secondary outcome measures related to the core symptoms of ASD.ResultsThere was no statistically significant improvement with oxytocin as compared to placebo on the ABC-SW (Mann-Whitney U=50, p=0.055), or on any secondary outcome measures, during either the double-blind or open-label phases. Oxytocin was generally well tolerated and there were no serious adverse events.Limitations: The small sample size, potential challenges with drug administration, and expectancy bias due to relying on parent reported outcome measures may all contribute to limitations in interpreting results.ConclusionOur results suggest that intranasal oxytocin is not efficacious in improving the core symptoms of ASD in children with PMS.Trial registration: NCT02710084
Wildfire Severity Zoning Through Google Earth Engine and Fire Risk Assessment: Application of Data Mining and Fuzzy Multi-Criteria Evaluation in Zagros Forests, Iran
Authors: Heidari Hamed , Keshtkar Mostafa , Moazzeni Niloofar , Jafari Meisam , Azadi Hossein
Published: March 2021

The arid and semi-arid regions of Zagros forests in the Middle East are constantly exposed to wildfire due to ecological conditions, and support systems are inefficient in controlling wildfires due to managerial and social weaknesses. Remote sensing and assessment tools are suitable for rapid prevention and action to identify the severity and location of a wildfire. This study investigated the natural resource management of Zagros Forestry in terms of protecting wildfire and combating forest wildfires using the NASA fire spatial data and the wildfire severity in the Google Earth Engine (GEE) platform. The land-use of the study area is produced by applying the Random Forest (RF) classification method and data from the Sentinel 2 satellite imagery for 2019. To separate the types of cultivation and vegetation of the region, the method of extracting the average vegetation index of the seasons is extracted from GEE. To evaluate fire risk, eleven human and ecological factors and two assessment models are applied to classify the probability fire risk therein. Furthermore, the outcome of AUC confirmed the Logistic Regression (LR) model; the accuracy of the LR (AUC=0.875049) model is satisfactory and is suitable for fire risk mapping in Zagros Forestry. Six high-risk areas of the wildfire were identified by MOLA, which overlap with protected areas. Out of a total of 20469.17 Ha of wildfire, 10426.41 Ha belong to these protected areas. 3826 Ha of this area were in the forests of Amygdalus spp, Quercus brant ii, pistacia Atlantica, and Quercus Infectoria, and 6600.41 Ha of it were in rangelands. Accordingly, an executive order was developed for the decision support system that reduces the risk of wildfire and helps extinguish the wildfire.
Causal Path of COPD Progression-Associated Genes in Different Biological Samples
Authors: Mostafaei Shayan , Borna Hojat , Emamvirdizadeh Alireza , Arabfard Masoud , Ahmadi Ali , Salimian Jafar , Salesi Mahmoud , jamalkandi Sadegh Azimzadeh
Published: March 2021

Chronic obstructive pulmonary disease (COPD) is a progressive inflammatory disease with pulmonary and extra-pulmonary complications. Due to the disease's systemic nature, many investigations investigated the genetic alterations in various biological samples. We aimed to infer causal genes in COPD's pathogenesis in different biological samples using elastic-net logistic regression and the Structural Equation Model. Samples of small airway epithelial cells, bronchoalveolar lavage macrophages, lung tissue biopsy, sputum, and blood samples were selected (135, 70, 235, 143, and 226 samples, respectively). Elastic-net Logistic Regression analysis was implemented to identify the most important genes involved in COPD progression. Thirty-three candidate genes were identified as essential factors in the pathogenesis of COPD and regulation of lung function. Recognized candidate genes in SAE cells have the highest area under the ROC curve (AUC=97%, SD= 3.9%). Our analysis indicates that macrophages and epithelial cells are more influential in COPD progression at the transcriptome level.
Abiraterone versus bicalutamide in combination with gonadotropin releasing hormone antagonist therapy for high risk metastatic hormone sensitive prostate cancer.
Authors: Ueda Takashi , Shiraishi Takumi , Ueda Saya , Ohashi Motoharu , Matsugasumi Toru , Yamada Yasuhiro , Fujihara Atsuko , Hongo Fumiya , Okihara Koji , Ukimura Osamu
Published: March 2021

ObjectivesTo compare the efficacy of abiraterone with that of bicalutamide in combination with gonadotropin-releasing hormone antagonist treatment for high risk metastatic hormone-sensitive prostate cancer patients.MethodsOne hundred and forty-nine patients with high risk metastatic hormone-sensitive prostate cancer at our hospital and affiliated hospitals between December 2013 and July 2020 were retrospectively identified. Fifty patients were administered abiraterone (1000mg/day) plus prednisolone (5mg/day) with gonadotropin-releasing hormone antagonist (degarelix) (group A) and 99 patients were administered bicalutamide (80mg/day) with gonadotropin-releasing hormone antagonist (group B). ResultsPSA- progression-free survival of group A was significantly longer than that of group B. Abiraterone therapy and Gleason score were significant independent factors for PSA-progression-free survival. By propensity score matching, total 56 matched patients were obtained. PSA-PFS (p<0.001) and OS (p=0.0071) of high risk mHNPC patients were significantly longer in abiraterone group of matched patients. Abiraterone therapy and Gleason score were still shown to be significant independent factors for PSA-PFS in matched patients.ConclusionsPSA-progression-free survival and overall survival in patients who were treated with abiraterone in combination with gonadotropin releasing hormone antagonist were significantly better than those of bicalutamide.
Virtual screening, molecular dynamics and binding energy-MM-PBSA studies of natural compounds to identify potential EcR inhibitors against Bemisia tabaci Gennadius
Authors: Mangat Harmilan Kaur , Rani Manisha , Pathak Rajesh Kumar , Yadav Inderjit Singh , Utreja Divya , Chhuneja Pardeep Kumar , Chhuneja Parveen
Published: March 2021

Whitefly (Bemisia tabaci Gennadius) is a hmpteran phyto polyphagous sucking insect pest which is an important pest of cotton that causes economic losses to the crop by reducing its yield and quality. Ecdysteroids like 20-hydroxy ecdysone (20-E), have significant role in larval moulting, development, and reproduction in pterygota insects. Intending to obstruct these fundamental, developmental physiological processes, the receptor of 20-E, the Ecdysone Receptor (BtEcR) of Bemisia tabaci has been targeted. To identify potent inhibitors of BtEcr, 98,072 natural compounds were retrieved from ZINC database. A structure-based virtual screening of these compounds was conducted for their binding to BtEcR and the top two compounds (ZINC08952607 and ZINC04264850) were selected based on minimum binding energy. 50 ns molecular dynamics simulation (MDS) study was then performed for the prediction of dynamics and stability of BtEcR and top-scoring ligand-BtEcR complexes. Besides, g_mmpbsa tool was used to calculate and analyse binding free energy of BtEcR-ligand complexes. The results revealed that ZINC08952607 and ZINC04264850 had binding free energy of −170.156 kJ/mol and −200.349 kJ/mol, respectively. Thus, these ligands can be utilized as lead compounds for the development of environmentally safe insecticides against the whitefly.
The Excitatory/Inhibitory Balance of Synaptic Transmission Is Impaired at Higher Extracellular Ca2+ Concentrations in Scn1a+/− Mouse Model of Dravet Syndrome
Authors: Uchino Kouya , Kawano Hiroyuki , Tanaka Yasuyoshi , Adaniya Yuna , Asahara Ai , Deshimaru Masanobu , Kubota Kaori , Watanabe Takuya , Katsurabayashi Shutaro , Iwasaki Katsunori , Hirose Shinichi
Published: March 2021

Dravet syndrome (DS) is an intractable form of childhood epilepsy that occurs in infancy. More than 80% of all patients have a heterozygous abnormality in the SCN1A gene, which encodes a subunit of Na+ channels in the brain. However, the detailed pathogenesis of DS remains unclear. This study investigated the synaptic pathogenesis of this disease in terms of excitatory/inhibitory balance using a mouse model of DS. We show that excitatory postsynaptic currents were similar between Scn1a knock-in neurons (Scn1a+/− neurons) and wild-type neurons, but inhibitory postsynaptic currents were significantly lower in Scn1a+/− neurons. Moreover, both the vesicular release probability and the number of inhibitory synapses were significantly lower in Scn1a+/− neurons compared with wild-type neurons. There was no proportional increase in inhibitory postsynaptic current amplitude in response to increased extracellular Ca2+ concentrations. Our study revealed that the number of inhibitory synapses is significantly reduced in Scn1a+/− neurons, while the sensitivity of inhibitory synapses to extracellular Ca2+ concentrations is markedly increased. These data suggest that Ca2+ tethering in inhibitory nerve terminals may be disturbed following the synaptic burst, likely leading to epileptic symptoms.