bluuebunny/crossref_metadata_2025 huggingface dataset:
Tooth Root Abscess and Mandibular Infections in Old World Camelids: 3 Cases
Authors: Sparnon Alyssa , Smith Joe , Mulon Pierre-Yves , Hecht Silke , Anderson David , Van Amstel Sarel
Published: April 2024

There are limited reports of mandibular infections and tooth root abscesses in camels (Old World Camelids). This is in contrast to multiple reports and case series detailing diagnosis, management, and therapy of similar infections in New World Camelids such as llamas and alpacas. The purpose of this case series is to present three cases of camels in North America with these infections and to detail the diagnostics, therapeutic interventions, management, and follow-up of these cases. Radiography was utilized in all three cases, as was sedation and/or anesthesia. Similar to llamas, florfenicol was used for antimicrobial therapy and flunixin meglumine was utilized as a nonsteroidal anti-inflammatory. Some degree of lavage was required for the management of each case. Clinicians should be aware of the potential need for sedation, diagnostic imaging, culture, and extended therapies for the treatment of mandibular and tooth root infections in camels as comparatively described for llamas and alpacas.
Mathematical Modelling of B2C Consumer Product Supply Strategy Based on Nonessential Demand Pattern
Authors: Zhuo Zhiyi , Chen Shuhong , Yan Hong
Published: April 2024

The influence of consumer psychological effects on customer needs has become a normal state of product sales models in the consumer goods supply chain field. The literature lacks systematic research on the mechanism through which consumer psychological effects affect customer needs. On the basis of analyzing and sorting out the literature and viewpoints, this paper establishes a mathematical model to investigate how manufacturers design and plan product supply strategies based on nonessential demand patterns under three different sales models. Finally, by solving the mathematical model, the optimal production volume, the optimal commodity price, and the maximum profit that can be obtained by the manufacturer are obtained, and the correctness of the model is verified via numerical calculation. The study results explain the relationships among commodity costs, demand, and commodity pricing, which are significant for the study of consumer psychology and can further provide a beneficial reference for manufacturers in various industries.
Adaptation of Research Project Requirement at Pharmacy Undergraduate Studies: Students’ Perception, Attitude, and Experiences
Authors: Rahim Najia , Rafiq Kiran , Nesar Shagufta , Naeem Sadaf , Anjum Fakhsheena , Mughal Muhammad Azhar
Published: April 2024

Objective. To determine the final year pharmacy undergraduate students’ attitudes toward research after completing a research project. Methods. A research project was introduced in the final year of the PharmD program in January 2022. After a period of one year, in Janurary 2023, students submitted their final research to the faculty members. The survey was conducted from 1st March to 30st April 2023 using a study tool that contained items asking students’ demographic, their research perceptions, attitude and experience, and also motivation/barriers faced during the research project. Descriptive and t-test statistics were utilized to compare the means of subgroups at a level of significance, i.e., p<0.05. The data were also analyzed using Goodman and Kruskal’s gamma and Mann–Whitney U test. Results. Majority of the students (93.8%) agreed regarding the significance of research in the pharmacy profession. Students were found to have their projects a worthwhile learning opportunity (94.2%). Students’ motivation to execute research project stems from mandatory curriculum courses, improving clinical or hospital pharmacist training and fulfilling research skills (90%). Barriers hindered include lack of training, time, and patient follow-up (approximately 70%). Conclusion. The current study's finding was concluded with the fact that research is a valuable component of a well-rounded education and can enhance a pharmacist's skills. However, they need a combination of formal education and practical experience to pursue a profession in pharmacy.
Supplementary Figure 5 from Elimusertib has Antitumor Activity in Preclinical Patient-Derived Pediatric Solid Tumor Models
Authors: Pusch Fabian F. , Dorado García Heathcliff , Xu Robin , Gürgen Dennis , Bei Yi , Brückner Lotte , Röefzaad Claudia , von Stebut Jennifer , Bardinet Victor , Chamorro Gonzalez Rocío , Eggert Angelika , Schulte Johannes H. , Hundsdörfer Patrick , Seifert Georg , Haase Kerstin , Schäfer Beat W. , Wachtel Marco , Kühl Anja A. , Ortiz Michael V. , Wengner Antje M. , Scheer Monika , Henssen Anton G.
Published: April 2024

Supplementary Figure 5 shows all tumor volume curves of the PDXs used in this study.
Supplementary Figure 11 from Elimusertib has Antitumor Activity in Preclinical Patient-Derived Pediatric Solid Tumor Models
Authors: Pusch Fabian F. , Dorado García Heathcliff , Xu Robin , Gürgen Dennis , Bei Yi , Brückner Lotte , Röefzaad Claudia , von Stebut Jennifer , Bardinet Victor , Chamorro Gonzalez Rocío , Eggert Angelika , Schulte Johannes H. , Hundsdörfer Patrick , Seifert Georg , Haase Kerstin , Schäfer Beat W. , Wachtel Marco , Kühl Anja A. , Ortiz Michael V. , Wengner Antje M. , Scheer Monika , Henssen Anton G.
Published: April 2024

Supplementary Figure 11 displays the quantification of each IHC marker in each PDX tested.
Supplementary Table S6 from Combination Therapy with EGFR Tyrosine Kinase Inhibitors and TEAD Inhibitor Increases Tumor Suppression Effects in EGFR Mutation–positive Lung Cancer
Authors: Ogimoto Tatsuya , Ozasa Hiroaki , Tsuji Takahiro , Funazo Tomoko , Yamazoe Masatoshi , Hashimoto Kentaro , Yoshida Hiroshi , Hosoya Kazutaka , Ajimizu Hitomi , Nomizo Takashi , Yoshida Hironori , Hamaji Masatsugu , Menju Toshi , Yoshizawa Akihiko , Date Hiroshi , Hirai Toyohiro
Published: April 2024

Supplementary Table S6 shows HALO configuration settings for immunohistochemistry.
Supplementary Figure S3 from Combination Therapy with EGFR Tyrosine Kinase Inhibitors and TEAD Inhibitor Increases Tumor Suppression Effects in EGFR Mutation–positive Lung Cancer
Authors: Ogimoto Tatsuya , Ozasa Hiroaki , Tsuji Takahiro , Funazo Tomoko , Yamazoe Masatoshi , Hashimoto Kentaro , Yoshida Hiroshi , Hosoya Kazutaka , Ajimizu Hitomi , Nomizo Takashi , Yoshida Hironori , Hamaji Masatsugu , Menju Toshi , Yoshizawa Akihiko , Date Hiroshi , Hirai Toyohiro
Published: April 2024

Supplementary Figure S3 shows cell viability assays of KTOR27 cells treated with afatinib and VT103.
Supplementary Figure 8 from Oncogenic Cells of Renal Embryonic Lineage Sensitive to the Small-Molecule Inhibitor QC6352 Display Depletion of KDM4 Levels and Disruption of Ribosome Biogenesis
Authors: Pichavaram Prahalathan , Jablonowski Carolyn M. , Fang Jie , Fleming Andrew M. , Gil Hyea Jin , Boghossian Andrew S. , Rees Matthew G. , Ronan Melissa M. , Roth Jennifer A. , Morton Christopher L. , Zambetti Gerard P. , Davidoff Andrew M. , Yang Jun , Murphy Andrew J.
Published: April 2024

QC6352 treatment (25nM x 72 hours) reduces expression of KDM4A in the nucleolus in WiT49 and HEK293. (A) Co-localization of KDM4A (red) and RNA polymerase I (green) is demonstrated in the nucleoli of WiT49 cells. QC6352 treatment caused (B) reduced expression of KDM4A in WiT49 cells (p<0.0001) and (C-D) reduced expression of KDM4A (p<0.0001) and RNA polymerase I (p=0.0370) in HEK293 cells.
Table S1 from Glucocorticoid Receptor (GR) Activation Is Associated with Increased cAMP/PKA Signaling in Castration-Resistant Prostate Cancer
Authors: Bennett Lynda , Jaiswal Praveen Kumar , Harkless Ryan V. , Long Tiha M. , Gao Ning , Vandenburg Brianna , Selman Phillip , Durdana Ishrat , Lastra Ricardo R. , Vander Griend Donald , Adelaiye-Ogala Remi , Szmulewitz Russell Z. , Conzen Suzanne D.
Published: April 2024

IPA-derived activation Z-scores in LAPC4 cells
Supplementary Data File 1 from Oncogenic Cells of Renal Embryonic Lineage Sensitive to the Small-Molecule Inhibitor QC6352 Display Depletion of KDM4 Levels and Disruption of Ribosome Biogenesis
Authors: Pichavaram Prahalathan , Jablonowski Carolyn M. , Fang Jie , Fleming Andrew M. , Gil Hyea Jin , Boghossian Andrew S. , Rees Matthew G. , Ronan Melissa M. , Roth Jennifer A. , Morton Christopher L. , Zambetti Gerard P. , Davidoff Andrew M. , Yang Jun , Murphy Andrew J.
Published: April 2024

RNA-seq gene set enrichment analysis for QC6352-treated WiT49 and HEK293 cells and controls
Supplementary Figure S4 from Cereblon-based Bifunctional Degrader of SOS1, BTX-6654, Targets Multiple KRAS Mutations and Inhibits Tumor Growth
Authors: Begovich Kyle , Schoolmeesters Angela , Rajapakse Navin , Martinez-Terroba Elena , Kumar Maneesh , Shakya Arvind , Lai Chon , Greene Steven , Whitefield Brandon , Okano Akinori , Mali Venkat , Huang Shenlin , Chourasia Aparajita H. , Fung Leah
Published: April 2024

Supplementary Figure S4 summarizes the exposure and changes of body weight for BTX-6654-treated mice
Supplementary Figure S3 from Cereblon-based Bifunctional Degrader of SOS1, BTX-6654, Targets Multiple KRAS Mutations and Inhibits Tumor Growth
Authors: Begovich Kyle , Schoolmeesters Angela , Rajapakse Navin , Martinez-Terroba Elena , Kumar Maneesh , Shakya Arvind , Lai Chon , Greene Steven , Whitefield Brandon , Okano Akinori , Mali Venkat , Huang Shenlin , Chourasia Aparajita H. , Fung Leah
Published: April 2024

Supplementary Figure S3 demonstrates the effects of genetic depletion of SOS1 in MIA PaCa-2 3D growth
Figure S1 from Neutrophil Elastase Remodels Mammary Tumors to Facilitate Lung Metastasis
Authors: Lulla Amriti R. , Akli Said , Karakas Cansu , Caruso Joseph A. , Warma Lucas D. , Fowlkes Natalie W. , Rao Xiayu , Wang Jing , Hunt Kelly K. , Watowich Stephanie S. , Keyomarsi Khandan
Published: April 2024

Supplementary Figure S1: (A) Fraction of CD45-ve cells in the bone marrows isolated from PyMT NE+/+ (N=5) and PyMT NE-/- (N=5), used in Supplementary Figure S2. Gating strategies for the CD45+ and CD45- cells is shown in Supplementary Figure S10. (B) Representative images of elastin-stained lungs in FVB NE+/+ and FVB NE-/- mice at 12 weeks of age. Graphs to the right shows quantitation of the NE mediated cleavage of elastin in each set of genotypes. (C) Lung metastasis evaluation in the 4T1 model of breast cancer. 0.5X106 cells were injected by tail vein injection in BALB/c NE+/+ (N=9) and BALB/c NE-/- (N=5) mice. Lung metastases were evaluated 14 days post tail vein injections in both genotypes. (D) 0.5X106 4T1 murine TNBC cell lines was injected in the T4 mammary fat pad of BALB/c NE+/+ (N=6) and BALB/c NE-/- (N=5) mice. Tumor growth curves, as a function of time are shown. (E) Baseline neutrophils fraction per mg of mammary gland in non-tumor bearing FVB NE-/- mice compared to BALB/c NE-/- mice. (F) Quantitative assessment of ductal staining and branch staining of mammary glands (from PyMT NE+/+ and PyMT NE-/- mice), using the Aperio color deconvolution algorithm.
Figure S2 from The Methyltransferases METTL7A and METTL7B Confer Resistance to Thiol-Based Histone Deacetylase Inhibitors
Authors: Robey Robert W. , Fitzsimmons Christina M. , Guiblet Wilfried M. , Frye William J.E. , González Dalmasy José M. , Wang Li , Russell Drake A. , Huff Lyn M. , Perciaccante Andrew J. , Ali-Rahmani Fatima , Lipsey Crystal C. , Wade Heidi M. , Mitchell Allison V. , Maligireddy Siddhardha S. , Terrero David , Butcher Donna , Edmondson Elijah F. , Jenkins Lisa M. , Nikitina Tatiana , Zhurkin Victor B. , Tiwari Amit K. , Piscopio Anthony D. , Totah Rheem A. , Bates Susan E. , Arda H. Efsun , Gottesman Michael M. , Batista Pedro J.
Published: April 2024

Figure S2 contains cell cycle analysis and cytotoxicity data for MCF7 DpVp300 cells showing they are resistant to romidepsin and do not overexpress P-gp
Data from Elimusertib has Antitumor Activity in Preclinical Patient-Derived Pediatric Solid Tumor Models
Authors: Pusch Fabian F. , Dorado García Heathcliff , Xu Robin , Gürgen Dennis , Bei Yi , Brückner Lotte , Röefzaad Claudia , von Stebut Jennifer , Bardinet Victor , Chamorro Gonzalez Rocío , Eggert Angelika , Schulte Johannes H. , Hundsdörfer Patrick , Seifert Georg , Haase Kerstin , Schäfer Beat W. , Wachtel Marco , Kühl Anja A. , Ortiz Michael V. , Wengner Antje M. , Scheer Monika , Henssen Anton G.
Published: April 2024

Abstract
The small-molecule inhibitor of ataxia telangiectasia and Rad3-related protein (ATR), elimusertib, is currently being tested clinically in various cancer entities in adults and children. Its preclinical antitumor activity in pediatric malignancies, however, is largely unknown. We here assessed the preclinical activity of elimusertib in 38 cell lines and 32 patient-derived xenograft (PDX) models derived from common pediatric solid tumor entities. Detailed in vitro and in vivo molecular characterization of the treated models enabled the evaluation of response biomarkers. Pronounced objective response rates were observed for elimusertib monotherapy in PDX, when treated with a regimen currently used in clinical trials. Strikingly, elimusertib showed stronger antitumor effects than some standard-of-care chemotherapies, particularly in alveolar rhabdomysarcoma PDX. Thus, elimusertib has strong preclinical antitumor activity in pediatric solid tumor models, which may translate to clinically meaningful responses in patients.
Supplementary Figure 3 from Identification of Colorectal Cancer Cell Stemness from Single-Cell RNA Sequencing
Authors: Lin Kangyu , Chowdhury Saikat , Zeineddine Mohammad A. , Zeineddine Fadl A. , Hornstein Nicholas J. , Villarreal Oscar E. , Maru Dipen M. , Haymaker Cara L. , Vauthey Jean-Nicolas , Chang George J. , Bogatenkova Elena , Menter David , Kopetz Scott , Shen John Paul
Published: April 2024

Single-cell stemness signature verification
Supplementary Figure 2 from Identification of Colorectal Cancer Cell Stemness from Single-Cell RNA Sequencing
Authors: Lin Kangyu , Chowdhury Saikat , Zeineddine Mohammad A. , Zeineddine Fadl A. , Hornstein Nicholas J. , Villarreal Oscar E. , Maru Dipen M. , Haymaker Cara L. , Vauthey Jean-Nicolas , Chang George J. , Bogatenkova Elena , Menter David , Kopetz Scott , Shen John Paul
Published: April 2024

Single-cell stemness signature identification
Figure S1 from miR-217 Regulates Normal and Tumor Cell Fate Following Induction of Endoplasmic Reticulum Stress
Authors: Dey Neekkan , Koumenis Costas , Ruggero Davide , Fuchs Serge Y. , Diehl J. Alan
Published: April 2024

S1. a) MiR-217 levels were quantified by qPCR following exposure of cells to Tg (300nM) and a small-molecule PERK inhibitor (left). PERK, ATF4 and CHOP induction were assessed by immunoblot (right). (b) eIF2S/S or eIF2A/A MEFs were treated with Tg (300nM) for indicated intervals and miR-217 levels were quantified by qPCR (right panels; n=3). (c) Co-expression analysis of miR-217 and EZH2 in different human cancers using ENCORI. (d) hsa-mir-217 cluster derived from miRbase.
Figure S8 from NRAS Mutant Dictates AHCYL1-Governed ER Calcium Homeostasis for Melanoma Tumor Growth
Authors: Cai Chufan , Tu Jiayi , Najarro Jeronimo , Zhang Rukang , Fan Hao , Zhang Freya Q. , Li Jiacheng , Xie Zhicheng , Su Rui , Dong Lei , Arellano Nicole , Ciboddo Michele , Elf Shannon E. , Gao Xue , Chen Jing , Wu Rong
Published: April 2024

Supplementary Figure 8. AHCYL1 deficiency causes cell growth attenuation, ER calcium decrease, and apoptosis in NRAS-Q61K overexpressed Mel-ST cells.
Figure S4 from NRAS Mutant Dictates AHCYL1-Governed ER Calcium Homeostasis for Melanoma Tumor Growth
Authors: Cai Chufan , Tu Jiayi , Najarro Jeronimo , Zhang Rukang , Fan Hao , Zhang Freya Q. , Li Jiacheng , Xie Zhicheng , Su Rui , Dong Lei , Arellano Nicole , Ciboddo Michele , Elf Shannon E. , Gao Xue , Chen Jing , Wu Rong
Published: April 2024

Supplementary Figure 4. AHCYL1 is selectively critical for KRAS-mutated but not for BRAF-mutated human colorectal cancer cells.
Figure S12 from NRAS Mutant Dictates AHCYL1-Governed ER Calcium Homeostasis for Melanoma Tumor Growth
Authors: Cai Chufan , Tu Jiayi , Najarro Jeronimo , Zhang Rukang , Fan Hao , Zhang Freya Q. , Li Jiacheng , Xie Zhicheng , Su Rui , Dong Lei , Arellano Nicole , Ciboddo Michele , Elf Shannon E. , Gao Xue , Chen Jing , Wu Rong
Published: April 2024

Supplementary Figure 12. AHCYL1 transcription in NRAS-mutated melanoma cells is related to MAPK14.
Data from RETRACTED: Minocycline Targets the NF-κB Nexus through Suppression of TGF-β1-TAK1-IκB Signaling in Ovarian Cancer
Authors: Ataie-Kachoie Parvin , Badar Samina , Morris David L. , Pourgholami Mohammad H.
Published: April 2024

Abstract
Substantial evidence supports the critical role of NF-κB in ovarian cancer. Minocycline, a tetracycline, has been shown to exhibit beneficial effects in this malignancy through regulation of a cohort of genes that overlap significantly with the NF-κB transcriptome. Here, it was examined whether or not the molecular mechanism could be attributed to modulation of NF-κB signaling using a combination of in vitro and in vivo models. Minocycline suppressed constitutive NF-κB activation in OVCAR-3 and SKOV-3 ovarian carcinoma cells and was correlated with attenuation of IκBα kinase (IKK) activation, IκBα phosphorylation and degradation, and p65 phosphorylation and nuclear translocation. The inhibition of IKK was found to be associated with suppression of TGF-β-activated-kinase-1 (TAK1) activation and its dissociation from TAK1-binding-protein-1 (TAB1), an indispensable functional mediator between TGF-β and TAK1. Further studies demonstrated that minocycline downregulated TGF-β1 expression. Enforced TGF-β1 expression induced NF-κB activity, and minocycline rescued this effect. Consistent with this finding, TGF-β1 knockdown suppressed NF-κB activation and abrogated the inhibitory effect of minocycline on this transcription factor. These results suggest that the minocycline-induced suppression of NF-κB activity is mediated, in part, through inhibition of TGF-β1. Furthermore, the influence of minocycline on NF-κB pathway activation was examined in female nude mice harboring intraperitoneal OVCAR-3 tumors. Both acute and chronic administration of minocycline led to suppression of p65 phosphorylation and nuclear translocation accompanied by downregulation of NF-κB activity and endogenous protein levels of its target gene products. These data reveal the therapeutic potential of minocycline as an agent targeting the pro-oncogenic TGF-β—NF-κB axis in ovarian cancer.

Implications: This preclinical study lends support to the notion that ovarian cancer management would benefit from administration of minocycline. Mol Cancer Res; 11(10); 1279–91. ©2013 AACR.
Supplementary Table 2 from The Immune Suppressor IGSF1 as a Potential Target for Cancer Immunotherapy
Authors: Koh Dong-In , Lee Minki , Park Yoon Sun , Shin Jae-Sik , Kim Joseph , Ryu Yea Seong , Lee Jun Hyung , Bae Seunggeon , Lee Mi So , Hong Jun Ki , Jeong Hong-Rae , Choi Mingee , Hong Seung-Woo , Kim Dong Kwan , Lee Hyun-kyung , Kim Bomi , Yoon Yoo Sang , Jin Dong-Hoon
Published: April 2024

Antibodies used in the experiments.
Supplementary Figure 6 from The Immune Suppressor IGSF1 as a Potential Target for Cancer Immunotherapy
Authors: Koh Dong-In , Lee Minki , Park Yoon Sun , Shin Jae-Sik , Kim Joseph , Ryu Yea Seong , Lee Jun Hyung , Bae Seunggeon , Lee Mi So , Hong Jun Ki , Jeong Hong-Rae , Choi Mingee , Hong Seung-Woo , Kim Dong Kwan , Lee Hyun-kyung , Kim Bomi , Yoon Yoo Sang , Jin Dong-Hoon
Published: April 2024

IGSF1 targeting antibody is developed and characterized by various methods.
Supplementary Figure 6 from Tumor Burden Dictates the Neoantigen Features Required to Generate an Effective Cancer Vaccine
Authors: Garzia Irene , Nocchi Linda , Avalle Lidia , Troise Fulvia , Leoni Guido , Seclì Laura , Antonucci Laura , Cotugno Gabriella , Allocca Simona , Romano Giuseppina , Conti Laura , Caiazza Carmen , Mallardo Massimo , Poli Valeria , Scarselli Elisa , D'Alise Anna Morena
Published: April 2024

Phenotype characterization of total CD8+ and nAg #23+ CD8+T cells in presence and absence of the “Help”. A) Schematic representation of the experimental set up. B) Representative FACS gating strategy for intratumoral CD8+ T cells. C-D) Phenotype characterization of CD8+ T cells infiltrating the tumors (C, n=14-17) and the tumor draining lymph nodes (D, dLN, n=5-7). The frequency of Exhausted, Stem-like and Better effector cells is shown in Help (blue bars) and No Help (red bars) groups. E) Representative FACS gating strategy for intratumoral nAg #23 specific CD8+ T cells. F-G) Phenotype characterization of intratumoral nAg #23 specific CD8+ T cells collected from mice immunized with Help and No Help vaccines at day 9 post vaccination (n=7-12). The frequency of #23+ specific CD8+ T cells (F) and Exhausted and Transitory (G) is shown in Help (blue bars) and No Help (red bars) groups. The phenotypic markers used to analyze each subtype are reported in the Material and Methods section. Results are from two independent experiments. Two-Way Anova with multiple comparison analysis or Mann Whitney test were performed between Help and No Help groups. *** p=0.0005; * p<0.05; ns = not significant.
Supplementary Figure 9 from Gut Mycobiota Dysbiosis Is Associated with Melanoma and Response to Anti–PD-1 Therapy
Authors: Szóstak Natalia , Handschuh Luiza , Samelak-Czajka Anna , Tomela Katarzyna , Pietrzak Bernadeta , Schmidt Marcin , Galus Łukasz , Mackiewicz Jacek , Mackiewicz Andrzej , Kozlowski Piotr , Philips Anna
Published: April 2024

Supplementary Figure 9. Relative abundance of Yarrowia lipolytica in overweight patients during anti-PD-1 treatment.
Supplementary Figure 8 from Gut Mycobiota Dysbiosis Is Associated with Melanoma and Response to Anti–PD-1 Therapy
Authors: Szóstak Natalia , Handschuh Luiza , Samelak-Czajka Anna , Tomela Katarzyna , Pietrzak Bernadeta , Schmidt Marcin , Galus Łukasz , Mackiewicz Jacek , Mackiewicz Andrzej , Kozlowski Piotr , Philips Anna
Published: April 2024

Supplementary Figure 8. Relative abundance of statistically significant gut fungi species in overweight and normal BMI patients (A) before anti-PD-1 treatment, and (B) in the third month of anti-PD-1 treatment.
Supplementary Figure 7 from Gut Mycobiota Dysbiosis Is Associated with Melanoma and Response to Anti–PD-1 Therapy
Authors: Szóstak Natalia , Handschuh Luiza , Samelak-Czajka Anna , Tomela Katarzyna , Pietrzak Bernadeta , Schmidt Marcin , Galus Łukasz , Mackiewicz Jacek , Mackiewicz Andrzej , Kozlowski Piotr , Philips Anna
Published: April 2024

Supplementary Figure 7. Relative abundance of selected species during anti-PD-1 treatment.
Supplementary Figure 1 from Dual Chimeric Antigen Receptor T Cells Targeting CD38 and SLAMF7 with Independent Signaling Demonstrate Preclinical Efficacy and Safety in Multiple Myeloma
Authors: Roders Nathalie , Nakid-Cordero Cecilia , Raineri Fabio , Fayon Maxime , Abecassis Audrey , Choisy Caroline , Nelson Elisabeth , Maillard Claire , Garrick David , Talbot Alexis , Fermand Jean-Paul , Arnulf Bertrand , Bories Jean-Christophe
Published: April 2024

Supplementary Figure 1
Figure S8. from High-Specificity CRISPR-Mediated Genome Engineering in Anti-BCMA Allogeneic CAR T Cells Suppresses Allograft Rejection in Preclinical Models
Authors: Degagné Émilie , Donohoue Paul D. , Roy Suparna , Scherer Jessica , Fowler Tristan W. , Davis Ryan T. , Reyes Gustavo A. , Kwong George , Stanaway Morena , Larroca Vicena Vanina , Mutha Devin , Guo Raymond , Edwards Leslie , Schilling Benjamin , Shaw McKay , Smith Stephen C. , Kohrs Bryan , Kufeldt Heinrich J. , Churchward Glen , Ruan Finey , Nyer David B. , McSweeney Kyle , Irby Matthew J. , Fuller Christopher K. , Banh Lynda , Toh Mckenzi S. , Thompson Matthew , Owen Arthur L.G. , An Zili , Gradia Scott , Skoble Justin , Bryan Mara , Garner Elizabeth , Kanner Steven B.
Published: April 2024

CB-011 immune-cloaking armoring strategy reduced NK cell proliferation and CAR+ T cell stress signatures.
Supplementary Figure S5 from Neutrophils Mediate Protection Against Colitis and Carcinogenesis by Controlling Bacterial Invasion and IL22 Production by γδ T Cells
Authors: Carnevale Silvia , Ponzetta Andrea , Rigatelli Anna , Carriero Roberta , Puccio Simone , Supino Domenico , Grieco Giovanna , Molisso Piera , Di Ceglie Irene , Scavello Francesco , Perucchini Chiara , Pasqualini Fabio , Recordati Camilla , Tripodo Claudio , Belmonte Beatrice , Mariancini Andrea , Kunderfranco Paolo , Sciumè Giuseppe , Lugli Enrico , Bonavita Eduardo , Magrini Elena , Garlanda Cecilia , Mantovani Alberto , Jaillon Sebastien
Published: April 2024

Figure S5. γδ T cell subsets activation in DSS-treated mice.
Related to Figure 4-5
A) Representative gating strategy for γδ T cell subsets in colon LP. B) Frequency of γδ T cell subsets from colon tissue of DSS-treated mice in colon LP. C) AhR expression in γδ T cells subset from colon LP of DSS-treated mice. D-E) Expression of IL-22 by γδ T cell subsets stimulated 4 hours with IL-23 plus IL-1β (D) and PMA plus ionomycin (E) analyzed by flow cytometry. F-G) Expression of IL-17 by γδ T cell subsets stimulated 4 hours with IL-23 plus IL-1β (F) and PMA plus ionomycin (G) analyzed by flow cytometry. H) RorγT expression in γδ T cell subsets from colon LP of DSS-treated mice. I) Body weight loss during DSS-induced acute colitis in Csf3r+/+(n=4), Csf3r-/- (n=4) and Csf3r-/- mice upon adoptive transfer of neutrophils (NΦ) (n=4) with and without antibiotic (ABX) oral treatment (Csf3r+/++ABX n=4 and Csf3r-/-+ABX n=4, Csf3r-/-+NΦ+ABX n=4). Red arrows indicated days of neutrophils transfer. A-H) Representative data of two independent experiments, Csf3r+/+(n=4), Csf3r-/- (n=4). I) One experiments. B-H) Multiple t-test. I) Wilcoxon matched-pairs signed rank test. Data are mean ± SEM. *** p < 0.001 ** p < 0.01 * p < 0.05.
Data from The Immune Suppressor IGSF1 as a Potential Target for Cancer Immunotherapy
Authors: Koh Dong-In , Lee Minki , Park Yoon Sun , Shin Jae-Sik , Kim Joseph , Ryu Yea Seong , Lee Jun Hyung , Bae Seunggeon , Lee Mi So , Hong Jun Ki , Jeong Hong-Rae , Choi Mingee , Hong Seung-Woo , Kim Dong Kwan , Lee Hyun-kyung , Kim Bomi , Yoon Yoo Sang , Jin Dong-Hoon
Published: April 2024

Abstract
The development of first-generation immune-checkpoint inhibitors targeting PD-1/PD-L1 and CTLA-4 ushered in a new era in anticancer therapy. Although immune-checkpoint blockade therapies have shown clinical success, a substantial number of patients yet fail to benefit. Many studies are under way to discover next-generation immunotherapeutic targets. Immunoglobulin superfamily member 1 (IGSF1) is a membrane glycoprotein proposed to regulate thyroid function. Despite containing 12 immunoglobin domains, a possible role for IGSF1, in immune response, remains unknown. Here, our studies revealed that IGSF1 is predominantly expressed in tumors but not normal tissues, and increased expression is observed in PD-L1low non–small cell lung cancer (NSCLC) cells as compared with PD-L1high cells. Subsequently, we developed and characterized an IGSF1-specific human monoclonal antibody, WM-A1, that effectively promoted antitumor immunity and overcame the limitations of first-generation immune-checkpoint inhibitors, likely via a distinct mechanism of action. We further demonstrated high WM-A1 efficacy in humanized peripheral blood mononuclear cells (PBMC), and syngeneic mouse models, finding additive efficacy in combination with an anti–PD-1 (a well-characterized checkpoint inhibitor). These findings support IGSF1 as an immune target that might complement existing cancer immunotherapeutics.
FIGURE 6 from Identification of Enhanced Vaccine Mimotopes for the p15E Murine Cancer Antigen
Authors: Zhou Shiqi , Song Yiting , Luo Yuan , Quinn Breandan , Jiao Yang , Long Mark D. , Abrams Scott I. , Lovell Jonathan F.
Published: April 2024

Generation of two-mutation p15E mimotopes that incorporate the 3C mutation. A, Strategy of e-mimotope screening for p15E-3C. (B) Peptide and MHC-I binding prediction by NetMHC algorithms. After vaccination on days 0 and day 7 with the indicated peptides with CPQ, blood was collected on day 14 for wild-type p15E-specific CD8+ T-cell frequency (C) as measured by tetramer staining. On day 14, 1 × 106 MC38 cells were inoculated subcutaneously. Mice received 2 µg peptide of the indicated liposomal vaccine on days 0 and day 7, splenocytes were harvested on day 14 and stimulated with the p15E wild-type peptide for IFNγ production by ELISOPOT (n = 5; D). On day 19 after tumor inoculation, tumor volume (E) was recorded (n = 3). Datapoints represent individual values of 3 mice from the same group in C and E or 5 mice from the same group in D. Statistical analysis applied one-way ANOVA followed by Tukey multiple comparisons tests. *, **, *** and **** indicating P < 0.05, 0.01, 0.001, and 0.0001, respectively. Error bars show mean ± SD.
Data from Identification of Enhanced Vaccine Mimotopes for the p15E Murine Cancer Antigen
Authors: Zhou Shiqi , Song Yiting , Luo Yuan , Quinn Breandan , Jiao Yang , Long Mark D. , Abrams Scott I. , Lovell Jonathan F.
Published: April 2024

Abstract
Mimotopes of short CD8+ T-cell epitopes generally comprise one or more mutated residues, and can increase the immunogenicity and function of peptide cancer vaccines. We recently developed a two-step approach to generate enhanced mimotopes using positional peptide microlibraries and herein applied this strategy to the broadly used H-2Kb–restricted murine leukemia p15E tumor rejection epitope. The wild-type p15E epitope (sequence: KSPWFTTL) was poorly immunogenic in mice, even when combined with a potent peptide nanoparticle vaccine system and did not delay p15E-expressing MC38 tumor growth. Following positional microlibrary functional screening of over 150 mimotope candidates, two were identified, both with mutations at residue 3 (p15E-P3C; “3C,” and p15E-P3M; “3M”) that better induced p15E-specific CD8+ T cells and led to tumor rejection. Although 3M was more immunogenic, 3C effectively delayed tumor growth in a therapeutic setting relative to the wild-type p15E. As 3C had less H-2Kb affinity relative to both p15E and 3M, 15 additional mimotope candidates (all that incorporated the 3C mutation) were assessed that maintained or improved predicted MHC-I affinity. Valine substitution at position 2 (3C2V, sequence: KVCWFTTL) led to improved p15E-specific immunogenicity, tumor rejection, and subsequent long-term antitumor immunity. 3C, 3M, and 3C2V mimotopes were more effective than p15E in controlling MC38 and B16-F10 tumors. T-cell receptor (TCR) sequencing revealed unique TCR transcripts for mimotopes, but there were no major differences in clonality. These results provide new p15E mimotopes for further vaccine use and illustrate considerations for MHC-I affinity, immunogenicity, and functional efficacy in mimotope design.
Significance:
The MHC-I–restricted p15E tumor rejection epitope is expressed in multiple murine cancer lines and is used as a marker of antitumor cellular immunity, but has seen limited success as a vaccine immunogen. An in vivo screening approach based on a positional peptide microlibraries is used to identify enhanced p15E mimotopes bearing amino acid mutations that induce significantly improved functional immunogenicity relative to vaccination with the wild-type epitope.
Advancements in HLA Typing Techniques and Their Impact on Transplantation Medicine
Authors: Geo Jeethu Anu , Ameen Reem , Al Shemmari Salem , Thomas Jibu
Published: April 2024

HLA typing serves as a standard practice in hematopoietic stem cell transplantation to ensure compatibility between donors and recipients, preventing the occurrence of allograft rejection and graft-versus-host disease. Conventional laboratory methods that have been widely employed in the past few years, including sequence-specific primer PCR and sequencing-based typing (SBT), currently face the risk of becoming obsolete. This risk stems not only from the extensive diversity within HLA genes but also from the rapid advancement of next-generation sequencing and third-generation sequencing technologies. Third-generation sequencing systems like single-molecule real-time (SMRT) sequencing and Oxford Nanopore (ONT) sequencing have the capability to analyze long-read sequences that span entire intronic-exonic regions of HLA genes, effectively addressing challenges related to HLA ambiguity and the phasing of multiple short-read fragments. The growing dominance of these advanced sequencers in HLA typing is expected to solidify further through ongoing refinements, cost reduction, and error rate minimization. This review focuses on hematopoietic stem cell transplantation (HSCT) and explores prospective advancements and application of HLA DNA typing techniques. It explores how the adoption of third-generation sequencing technologies can revolutionize the field by offering improved accuracy, reduced ambiguity, and enhanced assessment of compatibility in HSCT. Embracing these cutting-edge technologies is essential to advancing the success rates and outcomes of hematopoietic stem cell transplantation. This review underscores the importance of staying at the forefront of HLA typing techniques to ensure the best possible outcomes for patients undergoing HSCT.
Pitfalls in Respiratory Tract Cytopathology
Authors: Wheeldon Leonie , Maddox Anthony
Published: April 2024

Background: Cytopathology is integral to the investigation and diagnosis of respiratory disease, and, in the last decade or so, transbronchial needle aspiration by endobronchial ultrasound has made possible diagnosis and staging of malignant thoracic tumours at a single procedure. In addition, interventional teams increasingly include cytopathologists and cytotechnologists who, by providing rapid onsite evaluation, ensure efficient sampling of intrathoracic targets with the ultimate goal of accurate diagnosis as well as sufficient material for comprehensive predictive testing. Nonetheless, “traditional” cytological investigations such as bronchial washings, brushings, and lavages are still carried out for investigation of both suspected neoplastic and non-neoplastic conditions, and all these procedures still produce specimens in which florid benign cells mimic malignancy, while truly neoplastic cells lurk quietly in the background. Furthermore, even when neoplasia is not suspected, issues in preparation and interpretation may render a final assessment inaccurate and, therefore, clinically unhelpful or misleading. In this overview, we have tried to adopt a format partly modelled on the passage of a specimen from clinical acquisition to laboratory endpoint, thus taking in potential pitfalls in communication, clinical interaction, transport, and clinic-based preparation, as well as in morphology, immunocytochemistry, and suitability for predictive testing. It is not exhaustive but highlights areas that may frequently be encountered or are part of our personal experience. Summary: The account highlights potential pitfalls in respiratory cytopathology at key stages of the process from acquisition to reporting and presents these in both flow diagram and tabular form. We hope this is useful for the increasingly collaborative roles of cytotechnologist and cytopathologist and their wider involvement in the clinical investigative teams. Key Messages: Correct clinical and radiological information is crucially important and promotes the correct acquisition and processing of cytopathological specimens. Cross-discipline collaborative working ensures the most efficient use of the specimen such that diagnoses and predictive tests are performed on optimal material, reducing the potential for misinterpretation. Nonetheless, even with optimal material, morphological mimics and atypical antigen expression may mislead and render accurate diagnosis challenging.
Glomerulonephritis after Alemtuzumab Treatment for Multiple Sclerosis: A Report of Two Cases
Authors: Al-Muhaiteeb Abdullah , Alkeay Kamal , Altaleb Ahmad
Published: April 2024

Introduction:

Alemtuzumab, a humanized monoclonal antibody indicated for the treatment of adult patients with active relapsing-remitting multiple sclerosis (MS), has been associated with increased risk of autoimmune adverse events, including thyroid disorders, immune thrombocytopenia, and renal diseases. Renal immune-mediated adverse events, which has been reported in 0.3% of patients treated with alemtuzumab in MS clinical trials, typically occur within 39 months after the last drug administration. However, no consensus has been reached regarding the management of patients who develop glomerulonephritis after treatment with alemtuzumab.

Case Presentation:

We report the cases of two young adults with MS who developed biopsy-proven severe glomerulonephritis after alemtuzumab treatment. Both patients, including a 32-year-old female patient who developed membranous nephropathy and a 31-year-old male who developed drug-induced podocytopathy, were treated successfully with the calcineurin inhibitor tacrolimus followed by the anti-CD20 antibody rituximab.

Conclusion:

Regular renal function monitoring is required in patients who may rarely develop glomerulonephritis following treatment with alemtuzumab. There is no clear consensus on case management. In both cases, immunosuppressive therapy, which was necessary due to disease severity, resulted in successful remission, highlighting the potential utility of this approach.
Lysine Methyltransferase 5A Promotes the Progression of Growth Hormone Pituitary Neuroendocrine Tumors through the Wnt/β-Catenin Signaling Pathway
Authors: Li Junjun , Song Hao , Chen Ting , Zhang Sixi , Zhang Chao , Ma Chen , Zhang Lingye , Wang Tengfei , Qian Yuan , Deng Xingli
Published: April 2024

Introduction: Growth hormone (GH) secreting pituitary adenoma is considered one of the most harmful types of Pituitary Neuroendocrine Tumors (PitNETs). Our previous research has found that high expression of Lysine methyltransferase 5A (KMT5A) is closely related to the proliferation of PitNETs. The aim of this study was to investigate the role and molecular mechanism of KMT5A in the progression of GH PitNETs. Methods: Immunohistochemistry, qRT-PCR, and Western blot (WB) were used to assess the expression levels of KMT5A in human normal pituitary and GH PitNETs, as well as in rat normal pituitary and GH3 cells. Additionally, we utilized RNA interference technology and treatment with a selective KMT5A inhibitor to decrease the expression of KMT5A in GH3 cells. CCK-8, EdU, flow cytometry (FCM), clone formation, and WB assay were further employed to evaluate the impact of KMT5A on the proliferation of GH3 cells in vitro. A xenograft model was established to evaluate the role of KMT5A in GH PitNETs progression in vivo. Results: KMT5A was highly expressed in GH PitNETs and GH3 cells. Moreover, the reduction of KMT5A expression led to inhibited growth of GH PitNETs and increased apoptosis of tumor cells, as indicated by the findings from CCK-8, EdU, clone formation, and FCM assays. Additionally, WB analysis identified the Wnt/β-catenin signaling pathway as a potential mechanism through which KMT5A promotes GH PitNETs progression. Conclusion: Our research suggests that KMT5A may facilitate the progression of GH PitNETs via the Wnt/β-catenin signaling pathway. Therefore, KMT5A may serve as a potential therapeutic target and molecular biomarker for GH PitNETs.
PENENTUAN STRATEGI KEBERLANJUTAN TELEVISI DIGITAL LOKAL DI ERA KETIDAKPASTIAN
Authors: Hadirawati Hadirawati , Mutmainnah Laila , Rahayu Utari
Published: April 2024

Berbagai gangguan di era ketidakpastian mempengaruhi keberlanjutan televisi digital lokal. Oleh karena itu diadakanlah penelitian yang bertujuan untuk menentukan strategi keberlanjutan televisi digital lokal di era ketidakpastian. Penelitian ini diadakan Juli sampai dengan Agustus 2023 di Kabupaten Takalar dengan memilih Takalar TV sebagai objek penelitian. Data primer diperoleh melalui wawancara dan pengisian kuisioner oleh 20 orang pakar. Data sekunder diperoleh dari studi literatur. Metode penelitian yang digunakan adalah metode penelitian kualitatif dan kuantitatif yaitu Focus Group Discussion (FGD) dan dilanjutkan dengan analisis SWOT, yaitu  mengidentifikasi aspek kekuatan, kelemahan, peluang, dan ancaman yang dihadapi oleh Takalar TV dan dari penilaian kekuatan, kelemahan, peluang dan ancaman tersebut akan ditentukan strategi terpilih. Hasil yang diperoleh adalah nilai faktor internal yaitu -56 diperoleh dari total nilai faktor kekuatan dikurangi dengan total nilai faktor kelemahan. Nilai faktor eksternal yaitu 120 diperoleh dari total nilai faktor peluang dikurangi dengan total nilai faktor ancaman. Berdasarkan pada nilai faktor internal dan faktor eksternal maka strategi keberlanjutan televisi digital di era ketidakpastian adalah turn around, yaitu memaksimalkan kekuatan yang dimiliki untuk mengimbangi kelemahan dan merebut peluang yang ada. Wujud dari strategi turn around adalah meningkatkan keterlibatan masyarakat (engagement) dan memaksimalkan penggunaan media sosial pada penciptaan produk, pemasaran dan layanan. Kesimpulan dari penelitian ini adalah strategi keberlanjutan dari televisi digital lokal yaitu turn around. Strategi ini diterapkan untuk mengurangi resiko karena adanya kelemahan pada sumberdaya manusia dan keterbatasan dalam finansial dan peralatan.
ANALISIS DAYA TAHAN USAHA HORTIKUKTURA: TINJAUAN ASET DAN EKOSISTEM KEWIRAUSAHAAN
Authors: Subaedah Subaedah , Puspitasari Ayu , Agus Agus , Syamsari Syamsari
Published: April 2024

Penelitian tentang daya tahan usaha hortikultura diperlukan karena semua jenis usaha mengalami banyak gangguan sehingga ada usaha yang tutup dan ada yang tetap bertahan karena memiliki daya tahan usaha yang tinggi.  Tujuan penelitian ini adalah untuk menganalisis aset petani dan ekosistem kewirausahaan dalam membentuk daya tahan usaha hortikultura di era disrupsi.  Metode penelitian yang digunakan adalah deskriptif. Data diperoleh melalui wawancara,  kuisioner dan survey yang dilakukan pada bulan Agustus sampai September 2023 dengan melibatkan 50 responden yang berprofesi sebagai petani hortikultura dan telah menjalankan usaha minimal lima tahun. Lokasi penelitian ini adalah dataran tinggi Kabupaten Gowa yaitu Kecamatan Tombolopao.  Hasil penelitian adalah usaha hortikultura yang memiliki daya tahan tinggi ditunjukkan oleh beberapa indikator yaitu usaha tersebut telah bertahan hidup minimal lima tahun dan memiliki kinerja usaha yang baik yaitu  hasil yang diperoleh dari usaha tersebut mampu mensejahterakan pelaku usaha hortikultura.  Daya tahan usaha hortikulutra tinggi karena petani memiliki aset dan didukung oleh ekosistem kewirausahaan.  Aset petani yaitu aset sumberdaya manusia yang ditunjukkan oleh pendidikan mayoritas petani adalah sekolah menengah atas dan sarjana,  aktif menambah pengetahuan melalui berbagai media, usaha didukung oleh tenaga kerja dari dalam internal keluarga; aset sosial yaitu keikutsertaan petani pada kelompok tani;  aset alam berupa ketersediaan air dari alam;  aset fisik berupa ketersediaan lahan dan tanaman hortikultura dan adanya penyewaan peralatan;  aset finansial yang beragam yaitu keuntungan usaha, kredit dari perbankan, modal dari keluarga. Ekosistem  kewirausahaan yang mendukung yaitu  dukungan pemerintah dalam bentuk penyuluhan, alsintan, jalan tani, pupuk subsidi serta fasilitas publik lainnya.  Budaya yang mendukung yaitu gotong royong dalam membangun dan merawat fasilitas umum dan budaya tolong menolong dalam memenuhi kebutuhan hidup.  Adanya pasar yang beragam.  Kesimpulan penelitian ini adalah usaha hortikultura memiliki daya tahan tinggi dalam menghadapi berbagai gangguan usaha karena petani memiliki beberapa aset dan didukung oleh ekosistem kewirausahaan.
POLA NAFKAH PETANI CABAI MERAH DI DESA TUGU REJO, BENGKULU
Authors: Irawan Andi , Sianturia Lilis Suriani
Published: April 2024

Penelitian ini menganalisis aset nafkah, pendapatan, dan pola nafkah rumah tangga petani cabai merah di Desa Tugu Rejo, Kabupaten Kepahiang. 45 petani dipilih secara acak dari populasi 411 petani. Data dikumpulkan melalui kuesioner dan dianalisis menggunakan Excel. Mayoritas petani memiliki lahan gurem (69%) dan aset finansial yang baik (58%). Pendapatan rumahtangga rata-rata per bulan adalah Rp 4.694.690,74, dengan Rp 3.135.579,63 yang bisa ditabung. Pola nafkah mencakup diversifikasi on-farm, off-farm, dan non-farm. Hasil menunjukkan kecukupan pendapatan untuk kebutuhan pokok rumahtangga.
PEMBERIAN JAMU HERBAL SEBAGAI IMBUHAN PAKAN ALAMI TERHADAP INDEKS PRODUKSI AYAM BROILER
Authors: Jabbar Andi Batari K. , Syam Nurmiani
Published: April 2024

Penelitian ini bertujuan menganalisis pengaruh jamu herbal sebagai imbuhan pakan untuk melihat indeks produksi ayam broiler. Penelitian dirancang menggunakan Rancangan Acak Lengkap (RAL) dengan 4 perlakuan dan 5 ulangan yaitu (P0): kontrol, (P1): pemberian jamu herbal 1,5 ml/l, (P2): pemberian jamu herbal 2,5 ml/l, (P3): pemberian jamu herbal 3,5 ml/l. Jamu diberi setiap hari hingga ayam berusia 30 hari. Parameter yang diamati pada penelitian ini adalah bobot badan akhir, persentase ayam hidup, konversi pakan, dan indeks produksi. Hasil penelitian menunjukkan bahwa berdasarkan hasil uji Anova, jamu herbal tidak memberi pengaruh nyata (P<5%) pada bobot badan akhir, persentase ayam hidup, konversi pakan dan indeks produksi. Rata-rata indeks produksi broiler pada perlakuan P0 (381), P1 (370), P2 (369) dan P3 (384). Kesimpulan penelitian ini adalah dengan penambahan jamu herbal sebagai imbuhan pakan alami tidak memberi pengaruh nyata terhadap indeks produksi.
O paradoxo nas personagens femininas rodriguianas
Authors: Hamann Fernanda
Published: April 2024

Este artigo pretende colocar em questão a pecha de “misógino” frequentemente associada a Nelson Rodrigues. Para isso, propõe uma análise de sua obra dramatúrgica, especificamente quanto a procedimentos de construção de personagens femininas. Além de protagonizarem metade das peças escritas pelo autor, tais personagens se afastam de estereótipos sexistas e reducionistas. Em sua complexidade, representam mulheres desejantes, em conflito com os obstáculos sociais e morais à realização de seus anseios. Mulheres divididas, marcadas pela dimensão do paradoxo, cuja abordagem no artigo se distribui em três tópicos: 1. Nem Evas, nem Marias; 2. Meninas adultizadas e senhoras infantilizadas; 3. Amor e ódio entre mulheres da família. Destaca-se, ainda, a importância atribuída por Nelson Rodrigues ao desejo feminino, com seu caráter disruptivo, percebido como ameaça, em especial pelos personagens masculinos.
Editorial
Authors: Silva Anderson Gonçalves da , Otsuka Edu Teruki , Fonseca Maria Augusta
Published: April 2024

Editorial por Anderson Gonçalves da Silva, Edu Teruki Otsuka, Maria Augusta Fonseca.
A escrita de Nelson Rodrigues, um clássico da nossa modernidade
Authors: Lopes Angela Leite
Published: April 2024

Este artigo analisa a obra de Nelson Rodrigues a partir das características da sua escrita para ressaltar seu lugar de destaque como um clássico da nossa modernidade. O foco principal, entretanto, é a sua dramaturgia e, mais especificamente, os recursos de teatralidade com os quais constrói suas peças. Recursos sobre os quais o próprio autor discorre, mostrando o rigor de sua operação literária, no sentido amplo da palavra.
Nelson Rodrigues: tradição e modernidade
Authors: Passos Cleusa Rios Pinheiro
Published: April 2024

Apresentação por Cleusa Rios P. Passos.