bluuebunny/crossref_metadata_2025 huggingface dataset:
Carabid Beetles under the Influence of Megafires in Pine and Secondary Forests of Central European Russia
Authors: Aleksanov Victor V. , Alekseev Sergei K. , Ruchin Alexander B. , Esin Mikhail N.
Published: June 2024

The identification of patterns and mechanisms of wildfire effects on biodiversity is of significant conservation importance. The research was conducted in a zone of mixed and broad-leaved forests. Carabid beetles were studied in eight sample plots that varied in relation to the 2010 and 2021 fires through 2022 and 2023. A total of 8667 specimens of 108 species of carabid beetles were counted. In 2022, plots were clearly differentiated by the pyrogenic disturbance in terms of the carabid species structure. As the pyrogenic disturbance increases, the total abundance of brachypterous as well as herbivore species decreases sharply. The carabids that were the most prevalent in an undisturbed forest (Carabus arcensis, Carabus glabratus, Pterostichus oblongopunctatus, and Amara brunnea) demonstrated sensitivity to the 2010 fire. The pyrophilous species Pterostichus quadrifoveolatus and Sericoda quadripunctata were attracted to a moderately burned area. Poecilus lepidus is confined to areas burned in 2010 and not affected by the 2021 fire. A moderately burned area was characterized by increased abundance and number of species. In 2023, the degree of similarity of carabid populations between sample plots did not generally increase, but only the 2010 fire was identified as a significant factor in population differentiation. The abundance of carabids decreased in a moderately burned area and an unburned area near the fire edge. The abundance and number of species increased markedly in heavily burned areas due to open habitat species, while the abundance of Carabus arcensis and Amara brunnea decreased in unburned areas. Forest megafires threaten the biodiversity of carabid beetles in pine and secondary forests.
Biomaterials Design for Human Body Repair
Authors: Drevet Richard , Benhayoune Hicham
Published: June 2024

The global clinical demand for biomaterials is constantly increasing due to the aging population [...]
Left Ventricular and Atrial Deformation in Patients with Acute Decompensated Heart Failure: A Pilot Study
Authors: Jurica Jakub , Péč Martin Jozef , Cingel Marek , Bolek Tomáš , Barbierik Vachalcová Marianna , Horná Simona , Galajda Peter , Mokáň Marián , Samoš Matej
Published: June 2024

Aims: The aims of this study were to compare global longitudinal strain of the left ventricle (LV-GLS) and reservoir strain of the left atrium (R-LAS) values between patients with acute decompensation of chronic heart failure (HF) and a control group. Methods: Sixteen patients admitted to our ward for acute decompensation of HF were enrolled in this study. Transthoracic echocardiography (TTE) with two-dimensional speckle-tracking analysis (2D ST) was performed in each patient. The patients were divided into two subgroups according to the value of left ventricular ejection fraction (EF) using a cut-off value of ≤40% to distinguish heart failure with reduced ejection fraction (HFrEF) from heart failure with preserved ejection fraction (HFpEF). The control group consisted of 16 individuals without a history of cardiovascular disease, each of whom underwent 2D ST analysis as well. Results: We found that LV-GLS and R-LAS were significantly lower in both the HFrEF and HFpEF subgroups in comparison with the control group (LV-GLS: −13.4 ± 4.7% vs. −19.7 ± 2.5%, p ˂ 0.05; R-LAS: +12.2 ± 6.9% vs. +40.3 ± 7.4%, p ˂ 0.05). Furthermore, there was a significant difference in LV-GLS (−9.6 ± 3.2% vs. −15.2 ± 4.3%, p ˂ 0.05) but not in R-LAS (+13.7 ± 8.6% vs. +11.4 ± 6.2%) between the HFrEF and HFpEF subgroups. Conclusions: Our study demonstrated a significant difference in LV-GLS and R-LAS in all enrolled HF patients compared to the control group. There was also a significant difference in LV-GLS between the HFrEF and HFpEF subgroups.
Cryoballoon-Assisted Pulmonary Vein Isolation and Left Atrial Roof Ablation Using a Simplified Sedation Strategy without Esophageal Temperature Monitoring: No Notable Thermal Esophageal Lesions and Low Arrhythmia Recurrence Rates after 2 Years
Authors: Erkapic Damir , Roussopoulos Konstantinos , Aleksic Marko , Sözener Korkut , Kostev Karel , Rosenbauer Josef , Sossalla Samuel , Gündüz Dursun , Labenz Joachim , Tanislav Christian , Weipert Kay Felix
Published: June 2024

Background: Atrial fibrillation (AF) ablation is increasingly effective for managing heart rhythm but poses risks like esophageal fistulas. Minimizing esophageal thermal lesions while simplifying procedures is crucial. Methods: This prospective study involved 100 consecutive AF patients undergoing cryoballoon ablation with simplified sedation, without esophageal temperature monitoring. Patients with paroxysmal AF (Group A) received pulmonary vein isolation only, while those with persistent AF (Group B) also had left atrial roof ablation. Gastroesophageal endoscopy was performed post-procedure to detect lesions, and cardiological follow-ups were conducted at 3, 12, and 24 months. Results: The cohort included 69% men, with a median age of 65.5 years. Post-ablation endoscopy was performed in 92 patients; esophageal lesions were found in 1.1% of Group A and none of Group B. GERD was diagnosed in 14% of patients, evenly distributed between groups and not linked to lesion occurrence. Gastric hypomotility was observed in 16% of patients, with no significant difference between groups. At 24 months, arrhythmia-free survival was 88% in Group A and 74% in Group B. Conclusion: Cryoballoon-assisted pulmonary vein isolation, with or without additional left atrial roof ablation and without esophageal temperature monitoring during a simplified sedation strategy, shows low risk of esophageal thermal injury and effective ablation outcomes.
Impacts of Unhealthy Behaviors on Mental Health among Public Health Residents: The PHRASI Study
Authors: Gianfredi Vincenza , Stacchini Lorenzo , Minutolo Giuseppa , De Nicolò Valentina , Berselli Nausicaa , Ancona Angela , Catalini Alessandro , Cedrone Fabrizio
Published: June 2024

Unhealthy behaviors (UBs) can affect mental health. The most impactful of these are alcohol abuse, sedentary behavior, and sleep disturbances. Since these UBs are not well explored in medical residents, this study aims to overcome this gap, focusing on outcomes such as depression and anxiety. The Public Health Residents’ Anonymous Survey in Italy (PHRASI) is a nationwide cross-sectional study based on an 88-item questionnaire. UBs were explored through validated questionnaires: the Alcohol Use Disorders Identification Test-c (AUDIT-C), the International Physical Activity Questionnaire (IPAQ), and the Insomnia Severity Index (ISI). Depressive symptoms and anxiety were detected by Patient Health Questionnaire-9 (PHQ-9) and Generalized Anxiety Disorder-7 (GAD-7), respectively. Hierarchical cluster analysis described clusters using responses from the AUDIT-C, IPAQ, and ISI. A total of 375 participants were included in this study. Three distinct clusters (CL) were identified: CL1 was characterized by a moderate risk for alcohol abuse and high insomnia dissatisfaction, with a significantly higher frequency of depressive symptoms (46%, p < 0.001) and anxiety (53%, p < 0.001); CL2 had no risk for alcohol abuse, high sleep satisfaction, and better mental health profile, with the lowest prevalence of depressive symptoms (15%, p < 0.001); CL3 had the highest risk of alcohol abuse, the highest rate of physical activity (40%, p = 0.013), and the lowest prevalence of anxiety (27%, p < 0.001). Subjects belonging to CL1 required much more attention to prevent the worsening of mental outcomes. Interventions for promoting mental health should be addressed in all Public Health schools to create more sustainable working conditions.
Catheter Intervention in a Patient with Intracranial Aneurysms and Glanzmann Thrombasthenia Caused by a Novel Homozygous Likely Pathogenic Variant in the ITGA2B Gene
Authors: Boeckelmann Doris , von Dobeneck Lara , Henkes Hans , Eichler Hermann , Glonnegger Hannah , Zieger Barbara
Published: June 2024

Glanzmann Thrombasthenia (GT) is an inherited platelet disorder caused by defects in platelet integrin αIIbβ3 (GPIIb/IIIa), which is a platelet receptor essential for the binding of fibrinogen. This can lead to severe bleeding, especially after trauma or perioperatively, and to microcytic anemia because of chronic blood loss. We report on a 40-year-old female patient with extensive bleeding complications and platelet antibody formation who presented in Homburg and Freiburg for extensive platelet function analyses and molecular genetic analyses. According to platelet aggregometry, the patient had previously been diagnosed with Glanzmann Thrombasthenia (GT). In addition, an MRI scan had been performed due to an unsteady gait and had revealed bilateral para-ophthalmic aneurysms of both internal carotid arteries (ICAs). Assuming a 5% rupture risk per 5 years for each aneurysm, the patient was offered and accepted endovascular treatment. Next-generation sequencing (NGS) panel analysis identified a previously undescribed homozygous one-base-pair deletion in ITGA2B, which leads to a loss of function of the αIIb-subunit of the receptor. This case illustrates the difficulties that can arise regarding the treatment of patients with rare platelet bleeding disorders, and supports the importance of continuous medical care by a specialized hemophilia center for these patients.
Prognostic Role of Lymphocyte-to-Monocyte Ratio (LMR) in Patients with Intermediate-Stage Hepatocellular Carcinoma (HCC) Undergoing Chemoembolizations (DEM-TACE or cTACE) of the Liver: Exploring the Link between Tumor Microenvironment and Interventional Radiology
Authors: Minici Roberto , Venturini Massimo , Guzzardi Giuseppe , Fontana Federico , Coppola Andrea , Piacentino Filippo , Torre Federico , Spinetta Marco , Maglio Pietro , Guerriero Pasquale , Ammendola Michele ,   MGJR Research Team, Brunese Luca , Laganà Domenico
Published: June 2024

Inflammation-based scores are biomarkers of the crosstalk between the tumor microenvironment and the immune response. Investigating the intricate relationship between the tumor stromal microenvironment, biomarkers, and the response to transcatheter arterial chemoembolization (TACE) is essential for early identification of TACE refractoriness or failure, providing insights into tumor biology and facilitating personalized therapeutic interventions. This study addresses a dearth of recent literature exploring the prognostic significance of the preoperative LMR in individuals from western countries diagnosed with stage B hepatocellular carcinoma (HCC) undergoing drug eluting microspheres TACE (DEM-TACE) or conventional TACE (cTACE). This international multi-center retrospective analysis included consecutive patients with stage B HCC who underwent TACE from January 2017 to June 2023. The study evaluated the ability of the preoperative LMR to predict complete response (CR), objective response (OR), sustained response duration (SRD) exceeding 6 months, successful downstaging at 6 months, progression-free survival (PFS) at 6 months, and overall survival (OS) at 6 months. The study population included 109 HCC patients and it was divided into low LMR (LMR < 2.24) and high LMR (LMR ≥ 2.24) groups, according to ROC curve analysis to select the optimal LMR cut-off value. High LMR was associated with lower Hepatitis C prevalence, higher absolute lymphocyte count, and a trend toward lower alpha-fetoprotein. The group with high LMRs exhibited superior CR rates (14.9% vs. 0%), overall OR (43.2% vs. 14.3%), and better PFS at 6 months (75.7% vs. 45.7%). The LMR, specifically categorized as <2.24 and ≥2.24, emerged as a robust predictor for treatment response and short-term outcomes in patients with stage B HCC undergoing DEM- or c-TACE.
An Innovative Management Framework for Smart Horticulture—The Integration of Hype Cycle Paradigm
Authors: Boșcoianu Mircea , Pop Sebastian , Iagăru Pompilica , Cioca Lucian-Ionel , Iagăru Romulus , Petre Ioana Mădălina
Published: June 2024

The aim of this paper is to identify the possibilities of the implementation of the Innovative Management Framework for Intelligent Horticulture (IMFIH) by farmers with the aim of deepening the dynamics of innovation and technologic transfer processes related to the integration of the aerial work capability offered by mini UAV systems in precision horticulture. Starting from an aerial system for intelligent monitoring and smart horticulture applications, the research methodology is designed to understand the specific processes of this transfer of innovation in a field characterized by evolutionary dynamics and in the context of a lack of data. Thus, it is considered to be a mix of both quantitative and qualitative methods used in order to identify the needs and opinions of farmers regarding the possible use of the capabilities of mini UAV systems and especially how to access this capability. The obtained results showed the profile of the farmers interested in mini UAV systems for monitoring field crops and also the relevant factors for initiating/accessing them: specialized education, entrepreneurial education, area owned, ability to create partnerships, intention to access/develop mini UAV systems, and the existence of an integrated framework for analyzing the opportunities and restrictions of implementing mini UAV systems in precision horticulture applications. The integration of the Hype Cycle Paradigm (HCP) into the proposal of IMFIH led to the creation of the IMFIH-HCP as an innovative framework capable of stimulating the dissemination and transfer of knowledge and technology in the case of future horticultural applications of precision in an emerging market.
Perceptions of Students of Education Sciences on Research Ethics and (Re)Formation of the University Curriculum
Authors: Opriş Dorin
Published: June 2024

The study investigates perceptions of students of education sciences in Romania concerning issues related to research ethics, starting from recent opinions that consider the lack of adequate training in this field as a threat to higher education. The purpose of this paper is to contribute to a better knowledge of the training stage of which Bachelor, Master and doctorate students are undertaking. Respondents were invited to take part in a survey that included items that asked their opinion on the place of research ethics in the university curriculum, the need for a support person on research ethics issues, the importance of respecting research ethics for involvement in a research project, and the relationship between academic ethics and research ethics. The results show that students’ perception of the importance of research ethics positively changes with the level of studies. Doctoral students appreciate the support of an ethics advisor and significantly value the ethics component of a research project. Compliance with university ethics norms and compliance with research ethics is statistically significant in two of the three analyzed situations. The research shows the need for reconsideration at the institutional level to identify new training pathways in student research ethics.
Graphitic Carbon Nitride (g-C3N4) in Photocatalytic Hydrogen Production: Critical Overview and Recent Advances
Authors: Kyriakos Periklis , Hristoforou Evangelos , Belessiotis George V.
Published: June 2024

Graphitic carbon Nitride (g-C3N4) is one of the most utilized graphitic materials in hydrogen (H2) production via photocatalytic water splitting. Thus, a detailed critical overview, updated with the most recent works, has been performed on the synthesis methods, modification techniques, characterization, and mechanisms of g-C3N4 and g-C3N4-based composite materials, with the aim of clarifying the optimum course towards highly efficient hydrogen-producing photocatalysts based on this promising material. First, the synthesis methods for different morphologies of pure g-C3N4 (bulk, nanosheets, nanotubes and nanodots) are critically analyzed in detail for every step and parameter involved, with special mention regarding the modification methods of g-C3N4 (doping and composite formation). Next, the most common results of g-C3N4 characterization, regarding structural, morphological, optical, and electrical properties, are presented and analyzed. Then, a detailed critical survey of the mechanisms, using g-C3N4 and g-C3N4-based composites during photocatalytic activity, is performed with a focus on their effect on their hydrogen production capabilities via water splitting. This review aims to provide a clear image of all aspects regarding the use of g-C3N4 for photocatalysis, as well as a comprehensive guide for research targeted towards this promising graphitic material.
A Dynamic Analysis of Biomethane Reforming for a Solid Oxide Fuel Cell Operating in a Power-to-Heat System Integrated into a Renewable Energy Community
Authors: Calise Francesco , Cappiello Francesco Liberato , Cimmino Luca , Vicidomini Maria
Published: June 2024

This paper aims to develop a dynamic simulation model for the reduction of energy consumption through the use of organic waste from a residential district, supplied by a hybrid renewable energy plant. The proposed layout is based on a novel paradigm of a renewable energy community focused on the biocircular economy and a sustainable approach. The novelty with respect to the majority of papers developed in the literature on renewable energy communities lies in the use of both solar photovoltaic production and the organic fraction of municipal solid waste collected by the community. Energy production by biomass conversion and by photovoltaic fields shared among the buildings is used to satisfy in a sustainable manner the community loads for heating, cooling, and power. The district heating network is based on water loop heat pumps and air-to-air heat pumps and it includes the power-to-heat energy storage strategy. The biogas produced by the anaerobic digestion process is cleaned in order to supply a solid oxide fuel cell for the production of additional power, mainly during the hours of poor or null solar energy production. Then, the layout integrates several innovative topics, such as the power-to-heat strategy, the biocircular economy, the low-temperature district heating, the use of a solid oxide fuel cell, and a renewable energy community. The dynamic model of the proposed hybrid renewable layout is developed in the TRNSYS environment, but some innovative energy components, such as anaerobic digestion, the biogas upgrading unit, and the solid oxide fuel cell, are dynamically modeled in MATLAB and then integrated into the whole plant model. The proposed plant has been confirmed to be extremely profitable and able to obtain important energy savings, considering the achieved payback period of 4.48 years and the primary energy saving of 23%. This layout resulted in an interesting solution for pushing the development of smart and sustainable cities.
Modeling and Control Strategies for Energy Management in a Wastewater Center: A Review on Aeration
Authors: Jamaludin Mukhammad , Tsai Yao-Chuan , Lin Hao-Ting , Huang Chi-Yung , Choi Wonjung , Chen Jiang-Gu , Sean Wu-Yang
Published: June 2024

Effective modeling and management are critical in wastewater treatment facilities since the aeration process accounts for 65–70% of the overall energy consumption. This study assesses control strategies specifically designed for different sizes of WWTP, analyzing their economic, environmental, and energy-related effects. Small WWTPs see advantages from the utilization of on/off and proportional–integral–derivative (PID) control methods, resulting in 10–25% energy savings and the reduction in dissolved oxygen (DO) levels by 5–30%. Cascade control and model predictive control (MPC) improve energy efficiency by 15–30% and stabilize DO levels by 15–35% in medium-sized WWTPs. Advanced WWTPs that utilize technologies such as MPC integrated with artificial intelligence (AI) and machine learning (ML) can decrease energy usage by 30–40% and enhance DO levels by 35–40%. Life cycle assessment (LCA) demonstrates substantial decreases in greenhouse gas (GHG) emissions: 5–20% for small, 10–25% for medium, and 30–35% for large WWTPs. These findings illustrate the feasibility and expandability of these tactics in both controlled laboratory environments and real-world situations, emphasizing the significance of customized methods for improving energy efficiency and sustainability in wastewater treatment. Subsequent investigations should prioritize integrating renewable energy sources and resolving obstacles in developing nations to enhance wastewater treatment plants’ energy efficiency and sustainability.
Electromobility as a Challenge of Modern City Logistics—Indicator Analysis
Authors: Pokorska Agnieszka , Wiśniewski Tomasz
Published: June 2024

Electromobility is the challenge of modern cities. The following article explores this issue. The article presents the authors’ assessment of the impact of sustainable urban mobility indicators on the development of electromobility strategies in cities. The purpose of the paper is to describe which indicators of sustainable urban mobility may influence the development of electromobility strategies in cities by increasing the number of not only low-emission cars (electric and hybrid) and low-emission buses but also public bicycles. The hypothesis posited in the work is: sustainable urban mobility is an important factor in the development of electromobility in cities. Our literature analysis, expert research, and statistical analysis allowed us to prepare a possibly comprehensive set of indicators for sustainable urban mobility that have a potential impact on the development of electromobility strategies. We ran a thematic analysis of systematic literature reviews and collected around 50 specific types of indicators and measures of sustainable mobility. After consulting the indicator database with experts, we expanded a list of 21 indicators of sustainable mobility. Next, using regression analysis, it was determined which of the sustainable urban mobility indicators is the most important for the development of electromobility strategies in the three identified transport systems.
Impacts of High PV Penetration on Slovenia’s Electricity Grid: Energy Modeling and Life Cycle Assessment
Authors: Dimnik Jože , Topić Božič Jelena , Čikić Ante , Muhič Simon
Published: June 2024

The complexities of high PV penetration in the electricity grid in Slovenia based on targets proposed in national energy and climate plan were explored. Scenarios modeled an increase in installation power from 1800 MW in 2030 to 8000 MW in 2050. They were analyzed using energy modeling and life cycle assessment to assess the technical and environmental aspects of high PV grid penetration. The results showed that the increase in PV production from 2200 GWh (2030) to 11,090 GWh (2050) showed an unfavorable course of excess electricity in the system, resulting in the need for short-term and long-term storage strategies and exports of electricity. LCA analysis showed that penetration of a high share of PV results in a decrease in the impact category of global warming, which is higher in 2050 green scenarios that phase out coal and lignite electricity sources (80.5% decrease) compared to the 2020 baseline scenario. The increase in mineral resource scarcity can be observed with an increase in PV share when comparing the 2030 (50%) and 2050 (150%) BAU scenarios with the baseline scenario (2020). Factors such as environmental impacts, technical challenges, and the impact on the grid must be considered when implementing a decarbonization strategy.
DNA Barcodes for Wood Identification of Anatomically Similar Species of Genus Chamaecyparis
Authors: Kim Minjun , Im Seokhyun , Kim Tae-Jong
Published: June 2024

The genus Chamaecyparis comprises seven species (C. formosensis, C. hodginsii, C. lawsoniana, C. nootkatensis (Callitropsis nootkatensis), C. obtusa, C. pisifera, and C. thyoides). Accurate species identification is necessary for proper use and economic value of wood. Species identification of woods is generally based on anatomical analysis; however, C. obtusa and C. pisifera wood have similar microscopic morphology, which makes species identification impossible. Therefore, the molecular identification of species in wood of the genus Chamaecyparis is required. In this study, six candidate DNA barcode genes (trnP-GGG, ycf1b, clpP, accD, ycf2, and rps16) in the chloroplast of Chamaecyparis were identified with nucleotide diversity values higher than the arbitrary value of 0.02. Each gene was evaluated for species identification using phylogenetic analysis by genes registered at NCBI (42 sequences each for trnP-GGG, ycf1b, clpP, accD, and ycf2, and 50 sequences for rps16). The genes trnP-GGG, clpP, and rps16 could not be distinguished between C. pisifera and C. formosensis. However, ycf1b, accD, and ycf2 could be distinguished between all Chamaecyparis species. These results suggest the use of the chloroplast genes ycf1b, accD, and ycf2 as DNA barcodes for species identification in Chamaecyparis, including C. obtusa and C. pisifera, based on the reported genetic information to date.
Response of Soil Microbial Community in Different Forest Management Stages of Chinese fir Plantation
Authors: Liao Xiaoli , Chen Yifei , Huang Haifeng , Zhang Hao , Su Yi , Zheng Dexiang , Jin Shaofei
Published: June 2024

The cultivation of Cunninghamia lanceolata (Lamb.) Hook. (Chinese fir) plays a crucial role in enhancing ecological security through water resource preservation and carbon sequestration in China. The biotic and abiotic environmental conditions vary across different stages of plantation, thereby influencing soil nutrient levels and microbial dynamics. However, the interconnection between the soil nutrient cycle and microbial communities within Chinese fir plantations throughout their entire life cycle remains inadequately understood. In this study, conducted across various management stages of Chinese fir plantations in China (including the juvenile stage, first thinning stage, second thinning stage, mature stage, and over-mature stage), we examined the associations among soil organic carbon, soil physicochemical properties, soil enzyme activities, and soil microbial dynamics. Our results revealed that forest management practices significantly modify soil physicochemical properties and enzyme activities across all management stages. Specifically, the concentrations of total soil carbon, soil organic carbon, and soil microbial biomass carbon were notably higher in the over-mature stage compared to other management stages. At the genus level, the five highest contributors belonged to Subgroup2, AD3, Xanthobacteraceae, Elsterales, and Acidobacteriales for the bacterial community. For the fungal community at the genus level, the five highest contributors belonged to Ascomycota, Serendipita, Saitozyma, Mortieralla, and Venturiales. Moreover, anthropogenic thinning activities during the management phase substantially altered both stand and soil environments, as well as the structural characteristics of soil microbial communities. Soil cellulase, soil electrical conductivity (EC), and soil available phosphorus (AP) emerged as key factors influencing the relative abundance of major fungal communities, whereas soil total nitrogen, EC, and AP were identified as critical factors affecting the relative abundance of major bacterial phyla. More microbiological groups increased significantly in the juvenile stage and over-mature stage. Our findings elucidate the intricate relationships between the soil nutrient cycle and soil microbiological dynamics across various forest management stages within Chinese fir plantations.
Fire Resistance of One-Sided, Surface-Charred Silver Fir and European Ash Timber
Authors: Ebner David Hans , Barbu Marius-Catalin , Prokop Ondřej , Čermák Petr
Published: June 2024

The aim of this work was to investigate the fire resistance of silver fir (Abies alba L.) and European ash (Fraxinus excelsior) boards charred using the traditional yakisugi method and to compare the results with the fire resistance of non-charred boards as a reference and exploit its potential as a material with fire protection properties. After the boards were surface-charred on one side, specimens with different char thicknesses, resulting from their different position in the chimney, were selected from each wood species and subjected to analysis. Specimens with dimensions of 250 × 90 mm underwent a small flame test, those of 220 × 170 mm received indirect flame exposure by constant heat flux radiation from an infra-red emitter and those of 600 × 600 mm were subjected to a fire resistance test according to EN 1363-1:2020. The results of the small flame tests showed statistically significant fire resistance enhancement of specimens with 6 and 3 mm char-layer thickness in fir and ash wood, respectively, and a 110% and 75% improvement when compared to reference specimens. The constant heat flux radiation tests did not reveal any significant differences between the reference and charred specimens. The up-scaled fire resistance test, in which an assembled panel was exposed to flame, also indicated significant improvement. The reference burn-through time of fir and ash specimens was improved significantly with increasing char layer thickness, resulting in 10%–26% of fire resistance improvement for fir and 5%–12% for ash wood specimens. These results, based on the tests performed, suggest that the one-sided surface-charring of wood can enhance its fire resistance; however, this was mostly achieved in boards with the thickest char layer in both wood species studied and not all fire resistance indicators were considered. Further in-depth studies are required to better understand the complex behaviour of charred wood in response to fire.
Differences in Soil Microbial Communities across Soil Types in China’s Temperate Forests
Authors: Yuan Yuxiang , Li Xueying , Liu Fengqin , Tian Xiangyu , Shao Yizhen , Yuan Zhiliang , Chen Yun
Published: June 2024

Soil microorganisms are a crucial component of forest ecosystems because of their involvement in the decomposition of organic matter and nutrient cycling and their influence on plant growth and development. Soil type is a fundamental characteristic of soil. In the transitional forest regions from subtropical to temperate zones in China, various soil types can be found, including yellow-brown soils, brown soils, and cinnamon soils. However, the composition and distribution patterns of soil bacterial and fungal communities in different soil types remain uncertain. This study selected a 4.8-hectare plot in Baiyun Mountain Forest National Park, China. To explore the spatial distribution and ecological processes of soil microbial communities across three different soil types, Illumina sequencing was conducted. Results showed that the composition and assembly of bacterial and fungal communities varied substantially among different soil types. Bacteria were more influenced by environmental factors than fungi. Fungal communities consistently demonstrated greater stability compared to bacterial communities across the three soil types. Light was the main environmental factor driving the variation in the assembly of microbial communities among different soil types. This study demonstrates that there are differences in the composition and structure of soil microbial communities among different soil types, providing important insights into the management and sustainable development of soil microorganisms in temperate forests.
Differential Responses of Bacterial and Fungal Community Structure in Soil to Nitrogen Deposition in Two Planted Forests in Southwest China in Relation to pH
Authors: Hou Zheng , Zhang Xiaohua , Chen Wen , Liang Ziqi , Wang Keqin , Zhang Ya , Song Yali
Published: June 2024

Increased nitrogen deposition profoundly impacts ecosystem nutrient cycling and poses a significant ecological challenge. Soil microorganisms are vital for carbon and nutrient cycling in ecosystems; however, the response of soil microbial communities in subtropical planted coniferous forests to nitrogen deposition remains poorly understood. This study carried out a four-year nitrogen addition experiment in the subtropical montane forests of central Yunnan to explore the microbial community dynamics and the primary regulatory factors in two coniferous forests (P. yunnanensis Franch. and P. armandii Franch.) under prolonged nitrogen addition. We observed that nitrogen addition elicited different responses in soil bacterial and fungal communities between the two forest types. In P. yunnanensis Franch. plantations, nitrogen supplementation notably reduced soil bacterial α-diversity but increased fungal diversity. In contrast, P. armandii Franch. forests showed the opposite trends, indicating stand-specific differences. Nitrogen addition also led to significant changes in soil nutrient dynamics, increasing soil pH in P. yunnanensis Franch. forests and decreasing it in P. armandii Franch. forests. These changes in soil nutrients significantly affected the diversity, community structure, and network interactions of soil microbial communities, with distinct responses noted between stands. Specifically, nitrogen addition significantly influenced the β-diversity of fungal communities more than that of bacterial communities. It also reduced the complexity of bacterial interspecies interactions in P. yunnanensis Franch. forests while enhancing it in P. armandii Franch. forests. Conversely, low levels of nitrogen addition improved the stability of fungal networks in both forest types. Using random forest and structural equation modeling, soil pH, NH4+-N, and total nitrogen (TN) were identified as key factors regulating bacterial and fungal communities after nitrogen addition. The varied soil nutrient conditions led to different responses in microbial diversity to nitrogen deposition, with nitrogen treatments primarily shaping microbial communities through changes in soil pH and nitrogen availability. This study provides essential insights into the scientific and sustainable management of subtropical plantation forest ecosystems.
Automated Shape Correction for Wood Composites in Continuous Pressing
Authors: Lv Yunlei , Liu Yaqiu , Li Xiang , Lu Lina , Malik Adil
Published: June 2024

The effective and comprehensive utilization of forest resources has become the theme of the global “dual-carbon strategy”. Forestry restructured wood is a kind of wood-based panel made of wood-based fiber composite material by high-temperature and high-pressure restructuring–molding, and has become an important material in the field of construction, furniture manufacturing, as well as derivative processing for its excellent physical and mechanical properties, decorative properties, and processing performance. Taking Medium Density Fiberboard (MDF) as the recombinant material as the research object, an event-triggered synergetic control mechanism based on interventional three-way decision making is proposed for the viscoelastic multi-field coupling-distributed agile control of the “fixed thickness section” in the MDF continuous flat-pressing process, where some typical quality control problems of complex plate shape deviations including thickness, slope, depression, and bump tend to occur. Firstly, the idea of constructing the industrial event information of continuous hot pressing based on information granulation is proposed, and the information granulation model of the viscoelastic plate shape process mechanism is established by combining the multi-field coupling effect. Secondly, an FMEA-based cyber granular method for diagnosing and controlling the plate thickness diagnosis and control failure information expression of continuous flat pressing is proposed for the problems of plate thickness control failure and plate thickness deviation defect elimination that are prone to occur in the continuous flat-pressing process. The precise control of the plate thickness in the production process is realized based on event-triggered control to achieve the intelligent identification and processing of the various types of faults. The application test is conducted in the international mainstream production line of a certain type of continuous hot-pressing equipment for the production of 18 mm plate thickness; the synergistic effect is basically synchronized after 3 s, the control accuracy reaches 30%, and the average value of the internal bond strength is 1.40, which ensures the integrity of the slab. Practical tests show that the method in the actual production is feasible and effective, with detection and control accuracy of up to ±0.05 mm, indicating that in the production of E0- and E1-level products, the rate of superior products can reach more than 95%.
Genome-Wide Identification of MYB Gene Family in Peach and Identification of MYBs Involved in Carotenoid Biosynthesis
Authors: Liu Fengyi , Zheng Jiarui , Yi Yuwei , Yang Xiaoyan , Jiang Leiyu , Ye Jiabao , Zhang Weiwei , Xu Feng
Published: June 2024

Carotenoids are naturally occurring tetraterpenoids that play a key role in fruit coloration, and yellow peaches are one of the best sources of carotenoid intake. MYB transcription factors are one of the largest families in plants and play an important role in the regulation of plant secondary metabolite biosynthesis. However, peach MYB family genes have not been fully analyzed, and in particular, MYBs that regulate carotenoid biosynthesis have not been fully characterized. In this study, 190 peach MYB genes, containing 68 1R-MYBs, 118 2R-MYBs, 3 3R-MYBs, and 1 4R-MYB, were identified at the genome level using bioinformatics methods. These 190 MYBs were classified into 27 subfamilies based on their phylogenetic relationships with Arabidopsis thaliana MYB family members, and they were unevenly distributed across eight chromosomes. MYB genes of the same subfamily exhibit similar but not identical gene structures and conserved motifs. The promoter regions contain cis-acting elements associated with stress response, hormone response, and plant growth and development. There were 54 collinear pairs of MYB genes in the peach genome, compared with 233 and 221 collinear pairs with Rosa chinensis and Arabidopsis, respectively. Thirteen differentially expressed genes in the carotenoid biosynthesis pathway in yellow peach were identified by transcriptome sequencing and contained MYB binding sites on their promoters. Based on a phylogenetic analysis, we identified 13 PpMYBs that may be involved in carotenoid biosynthesis, and a correlation analysis revealed that they regulate carotenoid accumulation by positively or negatively regulating the expression of carotenoid biosynthetic genes. Further degradome sequencing screened that mdm-miR858 was able to target PpMYB17 and PpMYB126 involved in the regulation of carotenoid biosynthesis. Our findings provide new insights into the potential role of MYB transcription factors in carotenoid biosynthesis and provide a theoretical basis for their molecular mechanisms.
Improving Otsu Method Parameters for Accurate and Efficient in LAI Measurement Using Fisheye Lens
Authors: Tian Jiayuan , Liu Xianglong , Zheng Yili , Xu Liheng , Huang Qingqing , Hu Xueyang
Published: June 2024

The leaf area index (LAI) is an essential indicator for assessing vegetation growth and understanding the dynamics of forest ecosystems and is defined as the ratio of the total leaf surface area in the plant canopy to the corresponding surface area below it. LAI has applications for obtaining information on plant health, carbon cycling, and forest ecosystems. Due to their price and portability, mobile devices are becoming an alternative to measuring LAI. In this research, a new method for estimating LAI using a smart device with a fisheye lens (SFL) is proposed. The traditional Otsu method was enhanced to improve the accuracy and efficiency of foreground segmentation. The experimental samples were located in Gansu Ziwuling National Forest Park in Qingyang. In the accuracy parameter improvement experiment, the variance of the average LAI value obtained by using both zenith angle segmentation and azimuth angle segmentation methods was reduced by 50%. The results show that the segmentation of the front and back scenes of the new Otsu method is more accurate, and the obtained LAI values are more reliable. In the efficiency parameter improvement experiment, the time spent is reduced by 17.85% when the enhanced Otsu method is used to ensure that the data anomaly rate does not exceed 10%, which improves the integration of the algorithm into mobile devices and the efficiency of obtaining LAI. This study provides a fast and effective method for the near-ground measurement of forest vegetation productivity and provides help for the calculation of forest carbon sequestration efficiency, oxygen release rate, and forest water and soil conservation ability.
Evaluation of Essential Oils and Their Blends on the Fermentative Profile, Microbial Count, and Aerobic Stability of Sorghum Silage
Authors: Aniceto Elon S. , Oliveira Tadeu S. , Meirelles José R. , Silva Ismael N. , Mozelli Filho Elvanio J. L. , Gomes Raphael S. , Arévalo Juliana P. , Moraes Patrícia R.
Published: June 2024

This study aims to evaluate the effect of these essential oils and their blends on the fermentative profile, losses by gases and effluents, nutritional value, microbial count, and aerobic stability of sorghum silage. A completely randomized design was used with eight treatments and four repetitions. The evaluated treatments were the following: control (CON), without any essential oil; rosemary (Ros); tea tree (TT); citronella (Cit); Ros + TT (50% + 50%); Ros + Cit (50% + 50%); TT + Cit (50% + 50%); and Ros + TT + Cit (33% + 33% + 33%). A 1000 mg/kg dose of ensiled mass (as-fed basis) was used for each of the treatments. The addition of essential oils and their blends had a significant impact (p < 0.05) on the chemical composition of sorghum silage. Crude protein content increased (p < 0.001) with the use of essential oils and their blends. The Ros affected (p < 0.05) the fibrous fraction of sorghum silage. Neutral detergent fiber in vitro degradability was reduced (p = 0.003) when we used the blend TT + Cit compared to Ros and TT. We observed that only Ros did not reduce acetic acid concentration (p = 0.031) compared to the CON. The essential oils and their blends did not affect losses (p > 0.05). Lactic acid bacteria population increased (p = 0.039) when using the blend Ros + TT + Cit compared to the CON. However, the populations of entero-bacteria and fungi were not affected (p > 0.05) by the essential oils or their blends. For aerobic stability, we observed that Ros increased (p < 0.001) the air exposure time of the sorghum silage. Furthermore, the essential oils impacted the sorghum silage’s pH, which affected (p = 0.003) its aerobic stability. In conclusion, the essential oils did not reduce sorghum silage losses. However, the Ros improved the nutritional quality and aerobic stability of sorghum silage, while the blend Ros + TT + Cit increased the lactic acid bacteria count in the silage. More in-depth studies are needed to elucidate the action of essential oils as silage additives.
Impact of a Biopreservative Derived from Lactic Fermentation on Quality after Food Processing: A Case Study on Sliced Cooked Ham
Authors: Fioravante Guerra André , Gava Barreto Angela , Rodrigues Viviani Isabella , Marques Costa Lucas , Alberto Guerra Carlos , Corich Viviana , Giacomini Alessio , Lemos Junior Wilson José Fernandes
Published: June 2024

This study presents an innovative approach to enhancing the shelf life and maintaining the quality of sliced cooked ham through the application of a natural biopreservative derived from lactic fermentation. The biopreservative, at concentrations ranging from 1% to 3.5%, demonstrated substantial efficacy in microbial inhibition, keeping the microbial density low and relatively constant over time (p < 0.05). Remarkably, even at the lower concentration of 1%, the microbial growth rates were significantly reduced, with the treated samples showing notable stability over 24 days at both 7 °C and 25 °C. The microbial count in the treated with biopreservative group was significantly lower (3.19 log cfu/g) compared to the blank (4.59 log cfu/g) and control (5.01 log cfu/g) over 4 days at 7 °C. The shelf life of the ham was 24 days for the blank, 20 days for the control, and 101 days for the treated group at 7 °C. Moreover, colorimetric analysis revealed that the treated samples maintained better color stability, experiencing less variation in the hue angle and chroma, suggesting a protective effect against quality degradation over time. The successful application of the biopreservative aligns with the growing consumer demand for natural food additives and underscores the movement toward sustainable, health-conscious food preservation practices. The findings of this study indicate a promising avenue for the food industry to adopt environmentally friendly alternatives to synthetic additives, which could significantly influence future standards in food processing and preservation.
Food Neophobias in Spanish Adults with Overweight or Obesity by Sex: Their Association with Sociodemographic Factors and the Most Prevalent Chronic Diseases
Authors: del Campo Carmen , Bouzas Cristina , Monserrat-Mesquida Margalida , Tur Josep A.
Published: June 2024

Food neophobia has been defined as the reluctance to try new foods. Food neophobia is common in children and older people, but until now, scarce research has been carried out on food neophobia in the adult population. The aim of this study was to assess the most usual food neophobias in Spanish adults with overweight and obesity by sex, and their association with sociodemographic factors and the most prevalent chronic diseases. A cross-sectional observational study was carried out on adults (mean age of 43.5 ± 13.7 years old; n = 590; 50% female) with overweight or obesity. Their anthropometrics, adherence to the Mediterranean diet, age, educational level, economic level, smoking and sleeping habits, physical activity, chronic diseases, and food neophobias were assessed. The highest food neophobias in Spanish adults with overweight or obesity were directed toward vegetables, meat, fish, pulses, game meat, and fruits, mostly among females, with differences between sexes. Phobias of the soft texture of foods were also observed, without differences between sexes. Age, educational level, sleeping habits, and physical activity were directly related, and economical level and smoking were inversely related to food neophobia, mainly to healthy foods, and more obvious in males than in females. There were associations between body mass index (BMI) and chronic diseases and food neophobia. Adherence to the adaption of healthy and sustainable diets is low within food neophobics, increasing the risk of diet-related chronic diseases.