bluuebunny/crossref_metadata_2025 huggingface dataset:
Improving the Energy Efficiency of Vehicles by Ensuring the Optimal Value of Excess Pressure in the Cabin Depending on the Travel Speed
Authors: Panfilov Ivan , Beskopylny Alexey N. , Meskhi Besarion
Published: May 2024

This work is devoted to the study of gas-dynamic processes in the operation of climate control systems in the cabins of vehicles (HVAC), focusing on pressure values. This research examines the issue of assessing the required values of air overpressure inside the locomotive cabin, which is necessary to prevent gas exchange between the interior of the cabin and the outside air through leaks in the cabin, including protection against the penetration of harmful substances. The pressure boost in the cabin depends, among other things, on the external air pressure on the locomotive body, the power of the climate system fan, and the ratio of the input and output deflectors. To determine the external air pressure, the problem of train movement in a wind tunnel is considered, the internal and external fluids domain is considered, and the air pressure on the cabin skin is determined using numerical methods CFD based on the Navier–Stokes equations, depending on the speed of movement. The finite-volume modeling package Ansys CFD (Fluent) was used as an implementation. The values of excess internal pressure, which ensures the operation of the climate system under different operating modes, were studied numerically and on the basis of an approximate applied formula. In particular, studies were carried out depending on the speed and movement of transport, on the airflow of the climate system, and on the ratio of the areas of input and output parameters. During a numerical experiment, it was found that for a train speed of 100 km/h, the required excess pressure is 560 kPa, and the most energy-efficient way to increase pressure is to regulate the area of the outlet valves.
Comparative Growth and Bacoside Production in Diploid and Tetraploid Bacopa monnieri (L.) Wettst. Cultivated Indoors via Hydroponic and Soil Culture Systems
Authors: Inthima Phithak , Supaibulwatana Kanyaratt
Published: May 2024

Bacopa monnieri, a cognitive-enhancing herb crucial in health supplements, faces quality variations and contamination by toxic substances in conventional field cultivation, which hinders industrial use. Here, indoor cultivation of diploid (2x) and tetraploid (4x) B. monnieri using hydroponic and soil systems was studied. Soil cultivation promoted longer shoot lengths but resulted in lower biomass and chlorophyll contents compared to hydroponic cultivation. Conversely, soil cultivation significantly elevated total phenolics, total triterpenoids, bacoside A3, and bacopaside X contents in both lines, showing 1.7- to 3.3-fold increases over hydroponic cultivation. Furthermore, 4x plants grown in soil had higher bacopaside II and total bacoside contents than hydroponically grown plants, with 2- and 1.5-fold increases, respectively. Yet, no significant differences were observed in growth and pigment between 2x and 4x lines under the same system. Similarly, no significant differences in bioactive compound productions were found between 2x and 4x hydroponically grown plants. However, in soil, 4x plants exhibited higher total phenolic content, bacopaside II, and total bacoside contents compared to 2x plants. Interestingly, 2x plants grown in soil were the top performers for bacoside production per plant. These findings optimize cultivation practices to meet industry demands, warranting further research into large-scale production techniques.
A New Method Based on Lattice Boltzmann Method and Unsupervised Clustering for Identification of Urban-Scale Ventilation Corridors
Authors: Li Tianyu , Xie Peng
Published: May 2024

With the increase in urban development intensity, the urban climate has become an important factor affecting sustainable development. The role of urban ventilation corridors in improving urban climate has received widespread attention. Urban ventilation identification and planning based on morphological methods have been initially applied. Traditional morphological methods do not adequately consider the dynamic process of air flow, resulting in a rough evaluation of urban ventilation patterns. This study proposes a new urban-scale ventilation corridor identification method that integrates the Lattice Boltzmann method and the K-means algorithm. Taking Wuhan, China as the research area, an empirical study in different wind directions was conducted on a 20 m grid. The results showed that three levels of ventilation corridors (245.47 km2 in total) and two levels of ventilation obstruction areas (658.09 km2 in total) were identified to depict the ventilation pattern of Wuhan’s central urban area. The method proposed in this study can meet the needs of urban-scale ventilation corridor identification in terms of spatial coverage, spatial distribution rate and dynamic analysis. Compared with the classic least cumulative ventilation cost method, the method proposed in this study can provide more morphologic details of the ventilation corridors. This plays a very important role in urban planning based on urban ventilation theory.
A Novel Rock Mass Discontinuity Detection Approach with CNNs and Multi-View Image Augmentation
Authors: Yalcin Ilyas , Can Recep , Gokceoglu Candan , Kocaman Sultan
Published: May 2024

Discontinuity is a key element used by geoscientists and civil engineers to characterize rock masses. The traditional approach to detecting and measuring rock discontinuity relies on fieldwork, which poses dangers to human life. Photogrammetric pattern recognition and 3D measurement techniques offer new possibilities without direct contact with rock masses. This study proposes a new approach to detect discontinuities using close-range photogrammetric techniques and convolutional neural networks (CNNs) trained on a small amount of data. Investigations were conducted on basalts in Bala, Ankara, Türkiye. A total of 34 multi-view images were collected with a remotely piloted aircraft system (RPAS), and discontinuity lines were manually delineated on a point cloud generated from these images. The lines were back-projected onto the raw images to increase the amount of data, a process we call multi-view (3D) augmentation. We further evaluated radiometric and geometric augmentation methods, the contribution of multi-view augmentation to the proposed model, and the transfer learning performance of six different CNN architectures. The highest performance was achieved with U-Net + SE-ResNeXt-50 with an F1-score of 90.6%. The CNN model trained from scratch with local features also yielded a similar F1-score (91.7%), which is the highest performance reported in the literature.
Transcriptional Up-Regulation of FBXW7 by KCa1.1 K+ Channel Inhibition through the Nrf2 Signaling Pathway in Human Prostate Cancer LNCaP Cell Spheroid Model
Authors: Ohya Susumu , Kito Hiroaki , Kajikuri Junko , Yamaguchi Yohei , Matsui Miki
Published: May 2024

The tumor suppressor gene F-box and WD repeat domain-containing (FBXW) 7 reduces cancer stemness properties by promoting the protein degradation of pluripotent stem cell markers. We recently demonstrated the transcriptional repression of FBXW7 by the three-dimensional (3D) spheroid formation of several cancer cells. In the present study, we found that the transcriptional activity of FBXW7 was promoted by the inhibition of the Ca2+-activated K+ channel, KCa1.1, in a 3D spheroid model of human prostate cancer LNCaP cells through the Akt-Nrf2 signaling pathway. The transcriptional activity of FBXW7 was reduced by the siRNA-mediated inhibition of the CCAAT-enhancer-binding protein C/EBP δ (CEBPD) after the transfection of miR223 mimics in the LNCaP spheroid model, suggesting the transcriptional regulation of FBXW7 through the Akt-Nrf2-CEBPD-miR223 transcriptional axis in the LNCaP spheroid model. Furthermore, the KCa1.1 inhibition-induced activation of FBXW7 reduced (1) KCa1.1 activity and protein levels in the plasma membrane and (2) the protein level of the cancer stem cell (CSC) markers, c-Myc, which is a molecule degraded by FBXW7, in the LNCaP spheroid model, indicating that KCa1.1 inhibition-induced FBXW7 activation suppressed CSC conversion in KCa1.1-positive cancer cells.
Comparative Single Vesicle Analysis of Aqueous Humor Extracellular Vesicles before and after Radiation in Uveal Melanoma Eyes
Authors: Sirivolu Shreya , Peng Chen-Ching , Neviani Paolo , Xu Benjamin Y. , Berry Jesse L. , Xu Liya
Published: May 2024

Small extracellular vesicles (sEVs) have been shown to promote tumorigenesis, treatment resistance, and metastasis in multiple cancer types; however, sEVs in the aqueous humor (AH) of uveal melanoma (UM) patients have never previously been profiled. In this study, we used single particle analysis to characterize sEV subpopulations in the AH of UM patients by quantifying their size, concentration, and phenotypes based on cell surface markers, specifically the tetraspanin co-expression patterns of CD9, CD63, and CD81. sEVs were analyzed from paired pre- and post-treatment (brachytherapy, a form of radiation) AH samples collected from 19 UM patients. In post-brachytherapy samples, two subpopulations, CD63/81+ and CD9/63/81+ sEVs, were significantly increased. These trends existed even when stratified by tumor location and GEP class 1 and class 2 (albeit not significant for GEP class 2). In this initial report of single vesicle profiling of sEVs in the AH of UM patients, we demonstrated that sEVs can be detected in the AH. We further identified two subpopulations that were increased post-brachytherapy, which may suggest radiation-induced release of these particles, potentially from tumor cells. Further study of the cargo carried by these sEV subpopulations may uncover important biomarkers and insights into tumorigenesis for UM.
Machine Learning Identifies Key Proteins in Primary Sclerosing Cholangitis Progression and Links High CCL24 to Cirrhosis
Authors: Snir Tom , Greenman Raanan , Aricha Revital , Frankel Matthew , Lawler John , Saffioti Francesca , Pinzani Massimo , Thorburn Douglas , Mor Adi , Vaknin Ilan
Published: May 2024

Primary sclerosing cholangitis (PSC) is a rare, progressive disease, characterized by inflammation and fibrosis of the bile ducts, lacking reliable prognostic biomarkers for disease activity. Machine learning applied to broad proteomic profiling of sera allowed for the discovery of markers of disease presence, severity, and cirrhosis and the exploration of the involvement of CCL24, a chemokine with fibro-inflammatory activity. Sera from 30 healthy controls and 45 PSC patients were profiled with proximity extension assay, quantifying the expression of 2870 proteins, and used to train an elastic net model. Proteins that contributed most to the model were tested for correlation to enhanced liver fibrosis (ELF) score and used to perform pathway analysis. Statistical modeling for the presence of cirrhosis was performed with principal component analysis (PCA), and receiver operating characteristics (ROC) curves were used to assess the useability of potential biomarkers. The model successfully predicted the presence of PSC, where the top-ranked proteins were associated with cell adhesion, immune response, and inflammation, and each had an area under receiver operator characteristic (AUROC) curve greater than 0.9 for disease presence and greater than 0.8 for ELF score. Pathway analysis showed enrichment for functions associated with PSC, overlapping with pathways enriched in patients with high levels of CCL24. Patients with cirrhosis showed higher levels of CCL24. This data-driven approach to characterize PSC and its severity highlights potential serum protein biomarkers and the importance of CCL24 in the disease, implying its therapeutic potential in PSC.
Blastocyst-like Structures in the Peripheral Retina of Young Adult Beagles
Authors: Ikeda Tsunehiko , Jin Denan , Takai Shinji , Nakamura Kimitoshi , Nemoto Emika , Kojima Shota , Oku Hidehiro
Published: May 2024

In this immunohistological study on the peripheral retina of 3-year-old beagle dogs, excised retina specimens were immunostained with antibodies against nestin, Oct4, Nanog, Sox2, CDX2, cytokeratin 18 (CK 18), RPE65, and YAP1, as well as hematoxylin and DAPI, two nuclear stains. Our findings revealed solitary cysts of various sizes in the inner retina. Intriguingly, a mass of small round cells with scant cytoplasms was observed in the cavity of small cysts, while many disorganized cells partially occupied the cavity of the large cysts. The small cysts were strongly positive for nestin, Oct4, Nanog, Sox2, CDX2, CK18, and YAP1. RPE65-positive cells were exclusively observed in the tissue surrounding the cysts. Since RPE65 is a specific marker of retinal pigment epithelial (RPE) cells, the surrounding cells of the peripheral cysts were presumably derived from RPE cells that migrated intraretinally. In the small cysts, intense positive staining for nestin, a marker of retinal stem cells, seemed to indicate that they were derived from retinal stem cells. The morphology and positive staining for markers of blastocyst and RPE cells indicated that the small cysts may have formed structures resembling the blastocyst, possibly caused by the interaction between retinal stem cells and migrated RPE cells.
Effect of Hydroxyurea on Morphology, Proliferation, and Protein Expression on Taenia crassiceps WFU Strain
Authors: Rios-Valencia Diana G. , Estrada Karel , Calderón-Gallegos Arturo , Tirado-Mendoza Rocío , Bobes Raúl J. , Laclette Juan P. , Cabrera-Bravo Margarita
Published: May 2024

Flatworms are known for their remarkable regenerative ability, one which depends on totipotent cells known as germinative cells in cestodes. Depletion of germinative cells with hydroxyurea (HU) affects the regeneration of the parasite. Here, we studied the reduction and recovery of germinative cells in T. crassiceps cysticerci after HU treatment (25 mM and 40 mM of HU for 6 days) through in vitro assays. Viability and morphological changes were evaluated. The recovery of cysticerci’s mobility and morphology was evaluated at 3 and 6 days, after 6 days of treatment. The number of proliferative cells was evaluated using EdU. Our results show morphological changes in the size, shape, and number of evaginated cysticerci at the 40 mM dose. The mobility of cysticerci was lower after 6 days of HU treatment at both concentrations. On days 3 and 6 of recovery after 25 mM of HU treatment, a partial recovery of the proliferative cells was observed. Proteomic and Gene Ontology analyses identified modifications in protein groups related to DNA binding, DNA damage, glycolytic enzymes, cytoskeleton, skeletal muscle, and RNA binding.
Metabolic Profile and Lipid Metabolism Phenotype in Mice with Conditional Deletion of Hepatic BMAL1
Authors: Gu Weijia , Li Ting , Huang Yuxin , Wang Ruiqing , Zhang Lu , Chen Rucheng , Li Ran , Liu Cuiqing
Published: May 2024

The disruption of circadian rhythms (CRs) has been linked to metabolic disorders, yet the role of hepatic BMAL1, a key circadian regulator, in the whole-body metabolism and the associated lipid metabolic phenotype in the liver remains unclear. Bmal1 floxed (Bmal1f/f) and hepatocyte-specific Bmal1 knockout (Bmal1hep−/−) C57BL/6J mice underwent a regular feeding regimen. Hepatic CR, lipid content, mitochondrial function, and systemic metabolism were assessed at zeitgeber time (ZT) 0 and ZT12. Relevant molecules were examined to elucidate the metabolic phenotype. Hepatocyte-specific knockout of Bmal1 disrupted the expression of rhythmic genes in the liver. Bmal1hep−/− mice exhibited decreased hepatic TG content at ZT0, primarily due to enhanced lipolysis, reduced lipogenesis, and diminished lipid uptake. The β-oxidation function of liver mitochondria decreased at both ZT0 and ZT12. Our findings on the metabolic profile and associated hepatic lipid metabolism in the absence of Bmal1 in hepatocytes provides new insights into metabolic syndromes from the perspective of liver CR disturbances.
Antimicrobial Packaging for Plum Tomatoes Based on ZnO Modified Low-Density Polyethylene
Authors: Motelica Ludmila , Ficai Denisa , Oprea Ovidiu-Cristian , Trusca Roxana-Doina , Ficai Anton , Stelescu Maria Daniela , Sonmez Maria , Nituica Mihaela , Mustatea Gabriel , Holban Alina Maria
Published: May 2024

Food safety and quality are major concerns in the food industry. Despite numerous studies, polyethylene remains one of the most used materials for packaging due to industry reluctance to invest in new technologies and equipment. Therefore, modifications to the current materials are easier to implement than adopting whole new solutions. Antibacterial activity can be induced in low-density polyethylene films only by adding antimicrobial agents. ZnO nanoparticles are well known for their strong antimicrobial activity, coupled with low toxicity and UV shielding capability. These characteristics recommend ZnO for the food industry. By incorporating such safe and dependable antimicrobial agents in the polyethylene matrix, we have obtained composite films able to inhibit microorganisms’ growth that can be used as packaging materials. Here we report the obtaining of highly homogenous composite films with up to 5% ZnO by a melt mixing process at 150 °C for 10 min. The composite films present good transparency in the visible domain, permitting consumers to visualize the food, but have good UV barrier properties. The composite films exhibit good antimicrobial and antibiofilm activity from the lowest ZnO composition (1%), against both Gram-positive and Gram-negative bacterial strains. The homogenous dispersion of ZnO nanoparticles into the polyethylene matrix was assessed by Fourier transform infrared microscopy and scanning electron microscopy. The optimal mechanical barrier properties were obtained for composition with 3% ZnO. The thermal analysis indicates that the addition of ZnO nanoparticles has increased thermal stability by more than 100 °C. The UV-Vis spectra indicate a low transmittance in the UV domain, lower than 5%, making the films suitable for blocking photo-oxidation processes. The obtained films proved to be efficient packaging films, successfully preserving plum (Rome) tomatoes for up to 14 days.
Conservation of HLA Spike Protein Epitopes Supports T Cell Cross-Protection in SARS-CoV-2 Vaccinated Individuals against the Potentially Zoonotic Coronavirus Khosta-2
Authors: Martín-Galiano Antonio J. , López Daniel
Published: May 2024

Heterologous vaccines, which induce immunity against several related pathogens, can be a very useful and rapid way to deal with new pandemics. In this study, the potential impact of licensed COVID-19 vaccines on cytotoxic and helper cell immune responses against Khosta-2, a novel sarbecovirus that productively infects human cells, was analyzed for the 567 and 41 most common HLA class I and II alleles, respectively. Computational predictions indicated that most of these 608 alleles, covering more than 90% of the human population, contain sufficient fully conserved T-cell epitopes between the Khosta-2 and SARS-CoV-2 spike-in proteins. Ninety percent of these fully conserved peptides for class I and 93% for class II HLA molecules were verified as epitopes recognized by CD8+ or CD4+ T lymphocytes, respectively. These results show a very high correlation between bioinformatic prediction and experimental assays, which strongly validates this study. This immunoinformatics analysis allowed a broader assessment of the alleles that recognize these peptides, a global approach at the population level that is not possible with experimental assays. In summary, these findings suggest that both cytotoxic and helper cell immune protection elicited by currently licensed COVID-19 vaccines should be effective against Khosta-2 virus infection. Finally, by being rapidly adaptable to future coronavirus pandemics, this study has potential public health implications.
Architectural Framework to Enhance Image-Based Vehicle Positioning for Advanced Functionalities
Authors: Beti Iosif-Alin , Herghelegiu Paul-Corneliu , Caruntu Constantin-Florin
Published: May 2024

The growing number of vehicles on the roads has resulted in several challenges, including increased accident rates, fuel consumption, pollution, travel time, and driving stress. However, recent advancements in intelligent vehicle technologies, such as sensors and communication networks, have the potential to revolutionize road traffic and address these challenges. In particular, the concept of platooning for autonomous vehicles, where they travel in groups at high speeds with minimal distances between them, has been proposed to enhance the efficiency of road traffic. To achieve this, it is essential to determine the precise position of vehicles relative to each other. Global positioning system (GPS) devices have an intended positioning error that might increase due to various conditions, e.g., the number of available satellites, nearby buildings, trees, driving into tunnels, etc., making it difficult to compute the exact relative position between two vehicles. To address this challenge, this paper proposes a new architectural framework to improve positioning accuracy using images captured by onboard cameras. It presents a novel algorithm and performance results for vehicle positioning based on GPS and video data. This approach is decentralized, meaning that each vehicle has its own camera and computing unit and communicates with nearby vehicles.
Orange Peel Biochar–CdS Composites for Photocatalytic Hydrogen Production
Authors: Li Xiang , Zang Yuxin , Zhang Jindi , Zhang Lili , Zhang Jing , Huang Mengyang , Wang Jiaqiang
Published: May 2024

Orange peel biochar (C)-supported cadmium sulfide composites (CdS-C) were prepared by the combination of hydrothermal and calcination methods. The structure and morphology were characterized in detail by X-ray diffraction (XRD) and scanning electron microscopy (SEM), respectively. The CdS-C composite with 60% CdS exhibited the highest photocatalytic hydrogen production rate of 7.8 mmol·g−1·h−1, approximately 3.69 times higher than that of synthesized CdS without biochar. These results indicate that biochar derived from orange peel could be a low-cost, renewable, environmentally friendly, and metal-free co-catalyst for CdS, enhancing its photostability.
Hybrid Gold-Based Perovskite Derivatives: Synthesis, Properties, and Prospects in Photovoltaics
Authors: Liu Chang , Fu Xifeng , Nan Zi-Ang , Zhang Zilong , Meng Lingyi , Gao Peng
Published: May 2024

Hybrid gold-based perovskite derivatives typically exhibit low optical bandgaps and high optical absorption coefficients, rendering them promising for photovoltaic applications. In this study, we successfully synthesized six new hybrid gold-based perovskite derivatives, namely [(C6H8N2)(AuI4)(AuI2)](3AMPY), [(C6H14N2)(AuI4)(AuI2)](3AMP), [(C8H12N)(AuI4)](2PEAI), [(C4H14N2O)(AuI4)2](OBA), [(C6H18N2O2)3(AuI4)4(I3)2](DDA), and [(C10H26N2O3)(AuI4)(I3)](TOTA), through a straightforward and efficient hydrothermal method, achieving millimeter-sized single crystals. The structural analysis of the single crystals revealed variations in crystal structures arising from differences in constituent units and their spatial positioning relationships. First-principles calculations ascertained their high optical absorption coefficients in the visible light spectrum and indirect bandgap properties. Theoretical models indicated that the spectroscopic limited maximum efficiency (SLME) values of 3AMPY, 2PEAI, DDA, and TOTA approached approximately 30% in films of 0.5 μm thickness, signifying their potential candidacy as solar cell absorbers.
Investigation of Post-Transplant Mental Well-Being in Liver Transplant Recipients with Hepatic Encephalopathy
Authors: Saritaş Serdar , Tarlaci Sultan , Bulbuloglu Semra , Guneş Hüseyin
Published: May 2024

Objective: In this study, we aimed to examine the healing trend of hepatic encephalopathy after transplantation surgery in patients with liver failure. Method: We conducted this descriptive and cross-sectional study with the participation of liver transplant recipients. A personal information form, the West Haven Criteria (WHC), the Warwick-Edinburgh Mental Well-Being Scale (WEMWBS), and the Richmond Agitation Sedation Scale (RASS) were used for data collection. The data were analyzed using Chi-squared tests, ANOVA, and paired-samples t-tests. Results: As time progressed after liver transplantation, hepatic encephalopathy stages regressed (p < 0.01). We found that liver transplant recipients with end-stage hepatic encephalopathy were mostly within the first 6 months after transplantation, while patients with first-stage hepatic encephalopathy had received liver transplants more than 2 years ago (p < 0.01). Conclusions: The results of our study revealed that hepatic encephalopathy stages regressed after transplantation, but there was no complete recovery. This highlights the need to develop new treatment strategies other than liver transplantation for the treatment of hepatic encephalopathy.
Exploring the Effects of Local Air Pollution on Popliteal Artery Aneurysms
Authors: Leinweber Maria Elisabeth , Meisenbacher Katrin , Schmandra Thomas , Karl Thomas , Torsello Giovanni , Walensi Mikolaj , Geisbuesch Phillip , Schmitz-Rixen Thomas , Jung Georg , Hofmann Amun Georg
Published: May 2024

Objectives: A growing body of evidence highlights the effects of air pollution on chronic and acute cardiovascular diseases, such as associations between PM10 and several cardiovascular events. However, evidence of the impact of fine air pollutants on the development and progression of peripheral arterial aneurysms is not available. Methods: Data were obtained from the multicenter PAA outcome registry POPART and the German Environment Agency. Means of the mean daily concentration of PM10, PM2.5, NO2, and O3 concentrations were calculated for 2, 10, and 3650 days prior to surgery for each patient. Additionally, weighted ten-year averages were analyzed. Correlation was assessed by calculating Pearson correlation coefficients, and regression analyses were conducted as multiple linear or multiple logistic regression, depending on the dependent variable. Results: For 1193 patients from the POPART registry, paired air pollution data were available. Most patients were male (95.6%) and received open surgical repair (89.9%). On a regional level, the arithmetic means of the daily means of PM10 between 2000 and 2022 were neither associated with average diameters nor runoff vessels. Negative correlations for mean PAA diameter and mean NO2, as well as a positive correlation with mean O3, were found; however, they were not statistically significant. On patient level, no evidence for an association of mean PM10 exposure over ten years prior to inclusion in the registry and PAA diameter or the number of runoff vessels was found. Weighted PM10, NO2, and O3 exposure over ten years also did not result in significant associations with aneurysm diameter or runoff vessels. Short-term air pollutant concentrations were not associated with symptomatic PAAs or with perioperative complications. Conclusions: We found no indication that long-term air pollutant concentrations are associated with PAA size or severity, neither on a regional nor individual level. Additionally, short-term air pollution showed no association with clinical presentation or treatment outcomes.
RETRACTED: A Phase III Head-to-Head Study to Compare the Efficacy and Safety of Fexuprazan and Esomeprazole in Treating Patients with Erosive Esophagitis
Authors: Jeong Yuchul , Lee Beom , Han Se-Hyeon
Published: May 2024

Background: Fexuprazan (Fexuclue®; Daewoong Pharmaceutical Co., Ltd., Seoul, Korea) is a novel potassium-competitive acid blocker (P-CAB). This multi-center, randomized, double-blind, active-controlled, parallel-group, therapeutic confirmatory, phase III study was conducted to assess its efficacy and safety compared with esomeprazole (Nexium®; AstraZeneca, Gothenburg, Mölndal, Sweden) in Korean patients with erosive esophagitis (EE). Methods: This study evaluated patients diagnosed with EE at a total of 25 institutions in Korea between 13 December 2018 and 7 August 2019. After voluntarily submitting a written informed consent form, the patients were evaluated using a screening test and then randomized to either of the two treatment arms. The proportion of the patients who achieved the complete recovery of mucosal breaks at 4 and 8 weeks, the proportion of those who achieved the complete recovery of heartburn at 3 and 7 days and 8 weeks, and changes in the GERD–Health-Related Quality of Life Questionnaire (GERD-HRQL) scores at 4 and 8 weeks from baseline served as efficacy outcome measures. The incidence of treatment-emergent adverse events (TEAEs) and adverse drug reactions (ADRs) and the serum gastrin levels served as safety outcome measures. Results: The study population comprised a total of 231 patients (n = 231) with EE, including 152 men (65.80%) and 79 women (34.20%); their mean age was 54.37 ± 12.66 years old. There were no significant differences in the efficacy and safety outcome measures between the two treatment arms (p > 0.05). Conclusions: It can be concluded that the efficacy and safety of Fexuclue® are not inferior to those of esomeprazole in Korean patients with EE.
Deciphering Tumor Response: The Role of Fluoro-18-d-Glucose Uptake in Evaluating Targeted Therapies with Tyrosine Kinase Inhibitors
Authors: Kairemo Kalevi , Gouda Mohamed , Chuang Hubert H. , Macapinlac Homer A. , Subbiah Vivek
Published: May 2024

Background/Objectives: The inhibitory effects of tyrosine kinase inhibitors (TKIs) on glucose uptake through their binding to human glucose transporter-1 (GLUT-1) have been well documented. Thus, our research aimed to explore the potential impact of various TKIs of GLUT-1 on the standard [18F]FDG-PET monitoring of tumor response in patients. Methods: To achieve this, we conducted an analysis on three patients who were undergoing treatment with different TKIs and harbored actionable alterations. Alongside the assessment of FDG data (including SUVmax, total lesion glycolysis (TLG), and metabolic tumor volume (MTV)), we also examined the changes in tumor sizes through follow-up [18F]FDG-PET/CT imaging. Notably, our patients harbored alterations in BRAFV600, RET, and c-KIT and exhibited positive responses to the targeted treatment. Results: Our analysis revealed that FDG data derived from SUVmax, TLG, and MTV offered quantifiable outcomes that were consistent with the measurements of tumor size. Conclusions: These findings lend support to the notion that the inhibition of GLUT-1, as a consequence of treatment efficacy, could be indirectly gauged through [18F] FDG-PET/CT imaging in cancer patients undergoing TKI therapy.
Comparison between Conventional and Digital Workflow in Implant Prosthetic Rehabilitation: A Randomized Controlled Trial
Authors: Corsalini Massimo , Barile Giuseppe , Ranieri Francesco , Morea Edvige , Corsalini Tommaso , Capodiferro Saverio , Palumbo Rosario Roberto
Published: May 2024

The progress of digital technologies in dental prosthodontics is fast and increasingly accurate, allowing practitioners to simplify their daily work. These technologies aim to substitute conventional techniques progressively, but their real efficiency and predictability are still under debate. Many systematic reviews emphasize the lack of clinical RCTs that compare digital and traditional workflow. To address this evidence, we conducted a three-arm designed clinical RCT, which compares fully digital, combined digital, and analogic and fully analog workflows. We aimed to compare the clinical properties of each workflow regarding interproximal (IC) and occlusal contact (OC), marginal fit, impression time (IT), and patient satisfaction through a VAS scale. In total, 72 patients were included in the study. The IC and OC of the digital workflow were better than the others (p < 0.001), which obtained similar results. No difference between implant–abutment fit was observed (p = 0.5966). The IT was shorter in the digital workflow than the others (p < 0.001), which were similar. Patient satisfaction was higher in the digital workflow than in the conventional one. Despite the limitations, this study’s results support better accuracy and patient tolerance of digital workflow than of conventional techniques, suggesting it as a viable alternative to the latter when performed by clinicians experienced in digital dentistry.
Therapeutic Potential of Fungal Polysaccharides in Gut Microbiota Regulation: Implications for Diabetes, Neurodegeneration, and Oncology
Authors: Barcan Alexandru Stefan , Barcan Rares Andrei , Vamanu Emanuel
Published: May 2024

This review evaluates the therapeutic effects of polysaccharides derived from mushroom species that have medicinal and edible properties. The fungal polysaccharides were recently studied, focusing on their modulation of the gut microbiota and their impact on various diseases. The study covers both clinical and preclinical studies, detailing the results and highlighting the significant influence of these polysaccharides on gut microbiota modulation. It discusses the potential health benefits derived from incorporating these polysaccharides into the diet for managing chronic diseases such as diabetes, neurodegenerative disorders, and cancer. Furthermore, the review emphasizes the interaction between fungal polysaccharides and the gut microbiota, underscoring their role in modulating the gut microbial community. It presents a systematic analysis of the findings, demonstrating the substantial impact of fungal polysaccharides on gut microbiota composition and function, which may contribute to their therapeutic effects in various chronic conditions. We conclude that the modulation of the gut microbiota by these polysaccharides may play a crucial role in mediating their therapeutic effects, offering a promising avenue for further research and potential applications in disease prevention and treatment.
The Need and Opportunity to Update the Inventory of Plant Pathogenic Fungi and Oomycetes in Mexico
Authors: Tovar-Pedraza Juan Manuel , Solano-Báez Alma Rosa , Leyva-Mir Santos Gerardo , Tlapal-Bolaños Bertha , Camacho-Tapia Moisés , García-León Elizabeth , Ayala-Escobar Victoria , Nava-Díaz Cristian , Quezada-Salinas Andrés , Santiago-Santiago Víctor , Beltrán-Peña Hugo , Hernandez-Hernandez Maria Alondra , Juárez-Cruz Karla Jenifer , Márquez-Licona Guillermo
Published: May 2024

Mexico generates specific phytosanitary regulations for each product and origin to prevent the entry of quarantine pests and/or delay their spread within the national territory, including fungi and oomycetes. Phytosanitary regulations are established based on available information on the presence or absence of these pathogens in the country; however, the compilation and precise analysis of reports is a challenging task due to many publications lacking scientific rigor in determining the presence of a taxon of phytosanitary interest in the country. This review evaluated various studies reporting the presence of plant pathogenic fungi and oomycetes in Mexico and concluded that some lists of diseases and phytopathogenic organisms lack technical-scientific basis. Thus, it highlights the need and presents an excellent opportunity to establish a National Collection of Fungal Cultures and a National Herbarium for obligate parasites, as well as to generate a National Database of Phytopathogenic Fungi and Oomycetes present in Mexico, supported by the combination of morphological, molecular, epidemiological, pathogenicity, symptom, and micrograph data. If realized, this would have a direct impact on many future applications related to various topics, including quarantines, risk analysis, biodiversity studies, and monitoring of fungicide resistance, among others.
In Vitro Activitiy of Rezafungin in Comparison with Anidulafungin and Caspofungin against Invasive Fungal Isolates (2017 to 2022) in China
Authors: Yang Simin , Wan Feifei , Zhang Min , Lin Huiping , Hu Liang , Zhou Ziyi , Wang Dongjiang , Zhou Aiping , Ni Lijun , Guo Jian , Wu Wenjuan
Published: May 2024

The efficacy of different echinocandins is assessed by evaluating the in vitro activity of a novel antifungal, rezafungin, against invasive fungal isolates in comparison with anidulafungin and caspofungin. Using the broth microdilution (BMD) method, the susceptibility of 1000 clinical Candida isolates (including 400 C. albicans, 200 C. glabrata, 200 C. parapsilosis, 150 C. tropicalis and 50 C. krusei) and 150 Aspergillus isolates (100 A. fumigatus and 50 A. flavus) from the Eastern China Invasive Fungi Infection Group (ECIFIG) was tested for the antifungals including anidulafungin, rezafungin, caspofungin and fluconazole. The echinocandins showed strong activity against C. albicans that was maintained against fluconazole-resistant isolates. The GM MIC (geometric mean minimum inhibitory concentration) value of rezafungin was found to be comparable to that of anidulafungin or caspofungin against the five tested common Candida species. C. tropicalis exhibited higher resistance rates (about 8.67–40.67% in different antifungals) than the other four Candida species. Through the sequencing of FKS genes, we searched for mutations in echinocandin-resistant C. tropicalis isolates and found that all displayed alterations in FKS1 S654P. The determined MEC (minimal effective concentration) values against A. fumigatus and A. flavus for rezafungin (0.116 μg/mL, 0.110 μg/mL) are comparable to those of caspofungin (0.122 μg/mL, 0.142 μg/mL) but higher than for anidulafungin (0.064 μg/mL, 0.059 μg/mL). Thus, the in vitro activity of rezafungin appears comparable to anidulafungin and caspofungin against most common Candida and Aspergillus species. Rezafungin showed higher susceptibility rates against C. glabrata. Rezafungin indicates its potent activity for potential clinical application.
Long-Term Prognostic Impact of Stress Hyperglycemia in Non-Diabetic Patients Treated with Successful Primary Percutaneous Coronary Intervention
Authors: Savic Lidija , Mrdovic Igor , Asanin Milika , Stankovic Sanja , Lasica Ratko , Krljanac Gordana , Simic Damjan , Matic Dragan
Published: May 2024

Background: stress hyperglicemia (SH) is common in patients with ST-elevation myocardial infraction (STEMI). The aims of this study were to analyze the impact of SH on the incidence of all-cause mortality and major adverse cardiovascular events (MACE-cardiovascular death, nonfatal reinfarction, target vessel revascularization, and stroke) in STEMI patients without diabetes mellitus (DM) who have been treated successfully with primary PCI (pPCI). Method: we analyzed 2362 STEMI patients treated with successful pPCI (post-procedural flow TIMI = 3) and without DM and cardiogenic shock at admission. Stress hyperglycemia was defined as plasma glucose level above 7.8 mmol/L at admission. The follow-up period was 8 years. Results: incidence of SH was 26.9%. Eight-year all-cause mortality and MACE rates were significantly higher in patients with SH, as compared to patients without SH (9.7% vs. 4.2%, p < 0.001, and 15.7% vs. 9.4%, p < 0.001). SH was an independent predictor of short- and long-term all-cause mortality (HR 2.19, 95%CI 1.16–4.18, and HR 1.99, 95%CI 1.03–3.85) and MACE (HR 1.49, 95%CI 1.03–2.03, and HR 1.35, 95%CI 1.03–1.89). Conclusion: despite successful revascularization, SH at admission was an independent predictor of short-term and long-term (up to eight years) all-cause mortality and MACE, but its negative prognostic impact was stronger in short-term follow-up.
Effect of Negative Pulse on the Stability of Black Electrolytes for Magnesium Alloy Microarc Oxidation
Authors: Chen Bo , Tong Rui , Li Hongtao , Wang Wenqiang , Chen Xuanyu , Wang Hao , Yang Yifeng , Zhou Shiquan
Published: May 2024

The correlation between negative pulse and the black electrolyte properties of magnesium alloy micro-arc oxidation and the treated area was investigated by introducing a negative pulse electric field. The physical phase composition, microstructure, elemental distribution, and content of the coating were analyzed using X-ray diffraction (XRD), scanning electron microscopy (SEM), and energy dispersive spectroscopy (EDS). The results showed that the introduction of negative pulses favored the generation of MgO and MgSiO3 contents in the coatings, and an increase in the MgO phase was found in the coatings formed in the failed electrolytes; the microporous size and microcracks of the coatings were gradually and significantly reduced; the average consumption of Cu ions was 0.0453 g/L·dm2, which is only 26% of that in the unipolar condition; the introduction of the negative pulses significantly improved the “anomalous consumption” of Cu ions. The introduction of negative pulse can significantly improve the “abnormal consumption” of copper ions, which is attributed to the change in the electric field by negative pulse, which makes the cathode-enriched Cu ions migrate to the anode and reduces the reduction and precipitation of Cu ions at the cathode.
Magnetically Induced Two-Phonon Blockade in a Hybrid Spin–Mechanical System
Authors: Liu Hong-Yue , Yin Tai-Shuang , Chen Aixi
Published: May 2024

Phonon blockade is an important quantum effect for revealing the quantum behaviors of mechanical systems. For a nitrogen-vacancy center spin strongly coupled to a mechanical resonator via the second-order magnetic gradient, we show that the qubit driving can lead to the implementation of the two-phonon blockade, while the usual mechanical driving only allows for the appearance of a single-phonon blockade. As a signature, we investigate three-phonon antibunching with a simultaneous two-phonon bunching process by numerically calculating the second-order and third-order correlation functions. We also analyze in detail the influence of the system parameters (including the qubit driving strength, the dephasing rate of the qubit, as well as the thermal phonon number) on the quality of the two-phonon blockade effect. Our work provides an alternative method for extending the concept of a phonon blockade from a single phonon to multiphonon. It is of direct relevance for the engineering of multiphonon quantum coherent devices and thus has potential applications in quantum information processing.
An Iterated Local Search Heuristic for the Multi-Trip Vehicle Routing Problem with Multiple Time Windows
Authors: Wu Yinghui , Du Haoran , Song Huixin
Published: May 2024

This paper studies the multi-trip vehicle routing problem with multiple time windows, which extends the multi-trip vehicle routing problem by deciding not only the sequence of customers that each vehicle serves but also the service time window of each customer. It also requires that the delivery service time is within the selected time windows and that the total demand of the customers served by the vehicle on each trip does not exceed the maximum carrying capacity. For solving the studied problem, we develop a mixed integer linear programming model with the objective of minimizing the total travel distance of vehicles and design a tailored iterative local search heuristic. Within the framework of the iterative local search, an improved Solomon greedy insertion algorithm suitable for multiple time windows and multi-trip scenarios is designed to generate the initial solution, and local search operators such as Or-opt and Relocate, as well as Random Exchange perturbation operations, are also developed. The experiment results demonstrate the effectiveness of the proposed model and algorithm and confirm that by providing customers with multiple time windows option, carriers can flexibly plan vehicle routes and select appropriate service time windows, thereby reducing the number of vehicles used and the total distance travelled and improve delivery success.
Research on the Automatic Detection of Ship Targets Based on an Improved YOLO v5 Algorithm and Model Optimization
Authors: Sun Xiaorui , Wu Henan , Yu Guang , Zheng Nan
Published: May 2024

Because of the vast ocean area and the large amount of high-resolution image data, ship detection and data processing have become more difficult. These difficulties can be solved using the artificial intelligence interpretation method. The efficient and accurate detection ability of ship target detection has been widely recognized with the increasing application of deep learning technology. It is widely used in the practice of ship target detection. Firstly, we set up a data set concerning ship targets by collecting and training a large number of images. Then, we improved the YOLO v5 algorithm. The feature specify module (FSM) is used in the improved algorithm. The improved YOLO v5 algorithm was applied to ship detection practice under the framework of Anaconda. Finally, the training results were optimized, and the false alarm rate was reduced. The detection rate was improved. According to the statistics pertaining to experimental results with other algorithm models, the improved YOLO v5 algorithm can effectively suppress conflicting information, and the detection ability of ship details is improved. This work has accumulated valuable experience for related follow-up research.
Direct Yaw Moment Control for Distributed Drive Electric Vehicles Based on Hierarchical Optimization Control Framework
Authors: Hu Jie , Zhang Kefan , Zhang Pei , Yan Fuwu
Published: May 2024

Direct yaw moment control (DYC) can effectively improve the yaw stability of four-wheel distributed drive electric vehicles (4W-DDEVs) under extreme conditions, which has become an indispensable part of active safety control for 4W-DDEVs. This study proposes a novel hierarchical DYC architecture for 4W-DDEVs to enhance vehicle stability during ever-changing road conditions. Firstly, a vehicle dynamics model is established, including a two-degree-of-freedom (2DOF) vehicle model for calculating the desired yaw rate and sideslip angle as the control target of the upper layer controller, a DDEV model composed of a seven-degree-of-freedom (7DOF) vehicle model, a tire model, a motor model and a driver model. Secondly, a hierarchical DYC is designed combining the upper layer yaw moment calculation and low layer torque distribution. Specifically, based on Matlab/Simulink, improved linear quadratic regulator (LQR) with weight matrix optimization based on inertia weight cosine-adjustment particle swarm optimization (IWCPSO) is employed to compute the required additional yaw moment in the upper-layer controller, while quadratic programming (QP) is used to allocate four motors’ torque with the optimization objective of minimizing the tire utilization rate. Finally, a comparative test with double-lane-change and sinusoidal conditions under a low and high adhesion road surface is conducted on Carsim and Matlab/Simulink joint simulation platform. With IWCPSO-LQR under double-lane-change (DLC) condition on a low adhesion road surface, the yaw rate and sideslip angle of the DDEV exhibits improvements of 95.2%, 96.8% in the integral sum of errors, 94.9%, 95.1% in the root mean squared error, and 78.8%, 98.5% in the peak value compared to those without control. Simulation results indicate the proposed hierarchical control method has a remarkable control effect on the yaw rate and sideslip angle, which effectively strengthens the driving stability of 4W-DDEVs.