bluuebunny/crossref_metadata_2025 huggingface dataset:
Acknowledgement to Reviewers of Humanities in 2019
Authors: Humanities Editorial Office
Published: January 2020

The editorial team greatly appreciates the reviewers who have dedicated their considerable time and expertise to the journal’s rigorous editorial process over the past 12 months, regardless of whether the papers are finally published or not[...]
Bidirectional Associations between Objective Physical Activity and Sleep Patterns in Spanish School Children
Authors: Ávila-García Manuel , Femia-Marzo Pedro , Huertas-Delgado Francisco Javier , Tercedor Pablo
Published: January 2020

Physical activity (PA) and sleep contribute to better children’s health. Nonetheless, the bidirectional relationship between both of these health-related factors is unclear when using objective measures. The aims of this study were (1) to describe the PA (light PA and moderate-to-vigorous PA (MVPA) and sleep (duration, latency, and efficiency) patterns of children and compare them with recommendations, and (2) to analyze the bidirectional association between PA levels and sleep patterns in 470 Spanish children according to sex (average age of 8.4 (0.4) years, 51.9% boys). A tri-axial accelerometer and sleep logs were used to measure PA (light PA and MVPA) and sleep patterns (duration, latency, and efficiency) in the children for seven consecutive days. Linear mixed models were conducted to analyze the bidirectional association (PA → sleep and sleep → PA) adjusted for the child, the sex, the school, and the day of observation. The results showed that, overall, the children did not meet the sleep duration recommendations per day. Regarding the bidirectional association, increased light PA and MVPA during the day was related to decreased sleep duration but an improvement in sleep efficiency that night. However, sleep duration and sleep efficiency were only related negatively and positively to light PA the following day, respectively. Regarding sex, light PA was associated with decreased sleep duration in both sexes, although the average value was lower in boys. In addition, light PA was also related only to an improvement in sleep efficiency the same night in both sexes, with girls generally having more efficient sleep. More studies in a representative sample of children that use objective measures to corroborate these results are needed.
An Expanded Agenda for the Primary Prevention of Breast Cancer: Charting a Course for the Future
Authors: White Mary C. , Kavanaugh-Lynch Marion (Mhel) H. E. , Davis-Patterson Shauntay , Buermeyer Nancy
Published: January 2020

Advances in breast cancer science, early detection, and treatment have resulted in improvements in breast cancer survival but not in breast cancer incidence. After skin cancer, breast cancer is the most common cancer diagnosis in the United States. Each year, nearly a quarter million U.S. women receive a breast cancer diagnosis, and the number continues to rise each year with the growth in the population of older women. Although much remains to be understood about breast cancer origins and prevention, action can be taken on the existing scientific knowledge to address the systemic factors that drive breast cancer risk at the population level. The California Breast Cancer Research Program funded a team at Breast Cancer Prevention Partners (BCPP) to convene leaders in advocacy, policy, and research related to breast cancer prevention from across the state of California. The objective was the development of a strategic plan to direct collective efforts toward specific and measurable objectives to reduce the incidence of breast cancer. The structured, innovative approach used by BCPP to integrate scientific evidence with community perspectives provides a model for other states to consider, to potentially change the future trajectory of breast cancer incidence in the United States.
Determinants of Vitamin D Supplementation among Individuals with Type 1 Diabetes
Authors: Kamiński Mikołaj , Molenda Magdalena , Banaś Agnieszka , Uruska Aleksandra , Zozulińska-Ziółkiewicz Dorota
Published: January 2020

Half of the individuals with type 1 diabetes (T1DM) may present Vitamin D (VD) deficiency. There is little known about factors determining a decision on VD supplementation. The study aimed to determine the factors affecting vitamin D supplementation in people with T1DM. A cross-sectional survey study using the authors’ questionnaire paper and its digital version was performed. The questions involved data on the basic characteristics of the respondent, medical history, VD supplementation status, influence of the social environment, self-education, and the most important personal motivator for VD supplement use. Multivariate logistic regression analysis was performed. We collected a total of n = 184 papers and n = 550 digital complete surveys. From 734 total respondents, 62.0% declared VD supplementation. The main personal rationale for VD supplementation were recommendation of medical specialist 172 (37.8%) and self-education 135 (29.7%). The main reasons for non-supplementation of VD were lack of knowledge about VD 159 (57.0%) and lack of motivation 77 (27.6%). VD supplementation was independently associated with a family doctor (odds ratio (OR), 95% confidence interval (CI): 4.67, 2.32–9.40) or medical specialist recommendation (16.20, 9.57–27.43), and self-education (5.97, 3.90–9.13). Most Polish individuals with T1DM use VD supplements, and the decision is related to physicians’ recommendations and self-education.
Organizational Downsizing, Work Conditions, and Employee Outcomes: Identifying Targets for Workplace Intervention among Survivors
Authors: Frone Michael R. , Blais Ann-Renee
Published: January 2020

This study broadly assesses the association of organizational downsizing to work conditions and employee outcomes, and the extent to which work conditions mediate the association of downsizing to employee outcomes, thereby serving as targets for workplace intervention to reduce the harmful effects of downsizing on surviving workers. The cross-sectional data came from a national probability sample of 2297 U.S. workers. A parallel multiple-mediator model with multiple outcomes was estimated, adjusting for personal, occupational, geographic, and temporal covariates. Exposure to downsizing was the predictor. A set of 12 work conditions, representing four dimensions of the work environment, served as simultaneous mediators (Work Role: work demands, role conflict, role ambiguity, and work autonomy; Interpersonal Relationships: supervisor aggression, coworker aggression, friendship formation, and dysfunctional leadership; Rewards: distributive justice and promotion opportunities; Security: job insecurity and employment insecurity). A set of 16 employee consequences, representing five categories of outcomes, served as simultaneous outcomes (Inability to Detach from Work: negative work rumination and inability to unwind after work; Energetic Resource Depletion: physical, mental, and emotional work fatigue; Negative Affect: depression, anxiety, and anger; Positive Affect: happiness, confidence, and vigor; Health: physical and mental health; Work Attitudes: job satisfaction, organizational commitment, and turnover intentions). The results indicated that downsizing had an adverse association with nine of the 12 work conditions (higher levels of work demands, role conflict, supervisor aggression, dysfunctional leadership, job insecurity, and employment insecurity, and lower levels of friendship formation, distributive justice, and promotion opportunities) and all 16 employee outcomes. Moreover, the associations of downsizing to the employee outcomes were indirect, collectively mediated by the nine work conditions. This study provides the broadest evaluation of the deleterious effects of downsizing on U.S. workers surviving a downsizing, identifies affected work conditions that can serve as targets for workplace interventions, and provides insight into why organizational downsizing often fails to deliver anticipated financial and performance benefits to organizations. In terms of serving as targets for workplace intervention, some work conditions meditated the associations of downsizing to a broad set of employee outcomes, whereas other work conditions were specific to certain outcomes. The broad mediators should be targets of any intervention aimed at reducing the adverse effects of downsizing, with additional workplace targets depending on the class of outcomes to be addressed by the intervention.
Primary Health Care for Aboriginal Australian Women in Remote Communities after a Pregnancy with Hyperglycaemia
Authors: Wood Anna , MacKay Diana , Fitzsimmons Dana , Derkenne Ruth , Kirkham Renae , Boyle Jacqueline A. , Connors Christine , Whitbread Cherie , Welsh Alison , Brown Alex , Shaw Jonathan E. , Maple-Brown Louise
Published: January 2020

Background: Hyperglycaemia in pregnancy contributes to adverse outcomes for women and their children. The postpartum period is an opportune time to support women to reduce cardiometabolic and diabetes risk in subsequent pregnancies. Aims: To identify strengths and gaps in current care for Aboriginal women after a pregnancy complicated by hyperglycaemia. Methods: A retrospective review of the 12 month postpartum care provided by primary health centres in remote Australia in 2013–2014 identified 195 women who experienced hyperglycaemia in pregnancy (gestational diabetes (GDM) (n = 147), type 2 diabetes (T2D) (n = 39), and unclear diabetes status (n = 9)). Results: Only 80 women (54%) with GDM had postpartum glycaemic checks. Of these, 32 women were diagnosed with prediabetes (n = 24) or diabetes (n = 8). Compared to women with GDM, women with T2D were more likely to have their weight measured (75% vs. 52%, p <0.01), and smoking status documented as “discussed” (65% vs. 34%, p < 0.01). Most women (97%) accessed the health centre at least once in the 12 month postpartum period but, during these visits, only 52% of women had service provision, either structured or opportunistic, related to diabetes. Conclusion: High rates of dysglycaemia among women screened for T2D after GDM in the 12 month postpartum period highlight the need for increased screening and early intervention to prevent the development of T2D and its complications. Whilst a clear strength was high postpartum attendance, many women did not attend health services for diabetes screening or management.
Green Spaces, Land Cover, Street Trees and Hypertension in the Megacity of São Paulo
Authors: Moreira Tiana C. L. , Polizel Jefferson L. , Santos Itamar de Souza , Silva Filho Demóstenes F. , Bensenor Isabela , Lotufo Paulo A. , Mauad Thais
Published: January 2020

Proximity to green spaces has been shown to be beneficial to several cardiovascular outcomes in urban spaces. Few studies, however, have analyzed the relationship between these outcomes and green space or land cover uses in low–medium income megacities, where the consequences of rapid and inordinate urbanization impose several health hazards. This study used a subgroup of the dataset from The Brazilian Longitudinal Study of Adult Health ELSA-BRASIL (n = 3418) to identify the correlation between the medical diagnosis of hypertension and green spaces in the megacity of São Paulo. Land cover classification was performed based on the random forest algorithm using geometrically corrected aerial photography (orthophoto). Three different indicators of exposure to green spaces were used: number of street trees, land cover and number of parks within 1 km. We used logistic regression models to obtain the association of the metrics exposure and health outcomes. The number of street trees in the regional governments (OR = 0.937 and number of parks within 1 km (OR = 0.876) were inversely associated with a diagnosis of hypertension. Sixty-three percent of the population had no parks within 1 km of their residence. Our data indicate the need to encourage large-scale street tree planting and increase the number of qualified parks in megacities.
What Affects the Diffusion of New Energy Vehicles Financial Subsidy Policy? Evidence from Chinese Cities
Authors: Liu Weixing , Yi Hongtao
Published: January 2020

Designing and implementing effective new energy vehicle (NEV) policy are policy priorities for policymakers and energy policy scholars. However, the formulation, adoption, and diffusion of the NEV policies have not been fully examined in the extant literature. This article explores the mechanisms driving the diffusion of local financial subsidy policy for NEVs in China. In this context, we aim at analyzing the factors affecting the diffusion of local financial subsidies for NEVs in cities, to explain why some cities have taken the lead in adopting local financial subsidy policies for NEVs, while other cities have lagged behind. Based on a data set of 286 cities in China from 2009 to 2016, and with event history analysis (EHA) to analyze the strategic behaviors of local governments, we found that the number of the city’s neighbors that have adopted the NEV policy, the financial incentive policy of the provincial government, the administrative ranking of the city, the city’s financial situation and innovation capacity have a direct impact on whether the city adopts a local financial subsidy policy for NEVs. This study has practical implications for policymakers in designing and promoting the spread of NEV policies.
Current Knowledge and Future Perspectives on Mesenchymal Stem Cell-Derived Exosomes as a New Therapeutic Agent
Authors: Joo Hyeon Su , Suh Ju Hun , Lee Hyeon Ji , Bang Eun Song , Lee Jung Min
Published: January 2020

Mesenchymal stem cells (MSCs) are on the cusp of regenerative medicine due to their differentiation capacity, favorable culture conditions, ability to be manipulated in vitro, and strong immunomodulatory activity. Recent studies indicate that the pleiotropic effects of MSCs, especially their immunomodulatory potential, can be largely attributed to paracrine factors. Exosomes, vesicles that are 30-150 nanometers in diameter that function in cell-cell communication, are one of the key paracrine effectors. MSC-derived exosomes are enriched with therapeutic miRNAs, mRNAs, cytokines, lipids, and growth factors. Emerging evidences support the compelling possibility of using MSC-derived exosomes as a new form of therapy for treating several different kinds of disease such as heart, kidney, immune diseases, neural injuries, and neurodegenerative disease. This review provides a summary of current knowledge and discusses engineering of MSC-derived exosomes for their use in translational medicine.
Genome Editing for the Understanding and Treatment of Inherited Cardiomyopathies
Authors: Nguyen Quynh , Lim Kenji Rowel Q. , Yokota Toshifumi
Published: January 2020

Cardiomyopathies are diseases of heart muscle, a significant percentage of which are genetic in origin. Cardiomyopathies can be classified as dilated, hypertrophic, restrictive, arrhythmogenic right ventricular or left ventricular non-compaction, although mixed morphologies are possible. A subset of neuromuscular disorders, notably Duchenne and Becker muscular dystrophies, are also characterized by cardiomyopathy aside from skeletal myopathy. The global burden of cardiomyopathies is certainly high, necessitating further research and novel therapies. Genome editing tools, which include zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs) and clustered regularly interspaced short palindromic repeats (CRISPR) systems have emerged as increasingly important technologies in studying this group of cardiovascular disorders. In this review, we discuss the applications of genome editing in the understanding and treatment of cardiomyopathy. We also describe recent advances in genome editing that may help improve these applications, and some future prospects for genome editing in cardiomyopathy treatment.
Endophytic Isolates of Cordyceps fumosorosea to Enhance the Growth of Solanum melongena and Reduce the Survival of Whitefly (Bemisia tabaci)
Authors: Sun Tingfei , Shen Zhang , Shaukat Mobeen , Du Cailian , Ali Shaukat
Published: January 2020

This study reports the effects of seed treatment with Cordyceps fumosorosea on seed germination, growth, colonization of eggplant (Solanum melongena), and growth of Bemisia tabaci (feeding on fungal colonized eggplant leaves). Germination rates of eggplant seeds were similar among different treatments. The growth parameters such as root length, shoot length, and number of leaves) differed significantly after 15, 30, and 60 days of seed treatment. The total dry weight of eggplant in response to treatment with C. fumosorosea isolates increased significantly when compared with the control. Both isolates of C. fumosorosea colonized different plant tissues, although the extent of colonization decreased during the experimental period. The colonization of eggplants by both C. fumosorosea isolates resulted in a significant reduction of B. tabaci incidence. This study possibly provides the first report of increased plant growth and increased insect mortality in eggplants inoculated with C. fumosorosea isolates.
Observations of Near-Surface Mixing Behind a Headland
Authors: Spicer Preston , Huguenard Kimberly
Published: January 2020

Field observations were collected near the mouth of the Bagaduce River, Maine, in order to understand how complex features affect the intratidal and lateral variability of turbulence and vertical mixing. The Bagaduce River is a low-inflow, macrotidal estuary that features tidal islands, tidal flats and sharp channel bends. Profiles of salinity, temperature, and turbulent kinetic energy dissipation (ε) were collected for a tidal cycle across the estuary with a microstructure profiler. Lateral distributions of current velocities were obtained with an acoustic doppler current profiler. Results showed intratidal asymmetries in bottom-generated vertical eddy diffusivity and viscosity, with larger values occurring on ebb (Kz: 10−2 m2; Az: 10−2 m2/s) compared to flood (Kz: 10−5 m2/s; Az: 10−4 m2/s). Bottom-generated mixing was moderated by the intrusion of stratified water on flood, which suppressed mixing. Elevated mixing (Kz: 10−3 m2; Az: 10−2.5 m2/s) occurred in the upper water column in the lee of a small island and was decoupled from the bottom layer. The near-surface mixing was a product of an eddy formed downstream of a headland, which tended to reinforce vertical shear by laterally straining streamwise velocities. These results are the first to show near-surface mixing caused by vertical vorticity induced by an eddy, rather than previously reported streamwise vorticity associated with lateral circulation.
Dual Imaging Gold Nanoplatforms for Targeted Radiotheranostics
Authors: Silva Francisco , Paulo António , Pallier Agnès , Même Sandra , Tóth Éva , Gano Lurdes , Marques Fernanda , Geraldes Carlos F.G.C. , Castro M. Margarida C.A. , Cardoso Ana M. , Jurado Amália S. , López-Larrubia Pilar , Lacerda Sara , Cabral Campello Maria Paula
Published: January 2020

Gold nanoparticles (AuNPs) are interesting for the design of new cancer theranostic tools, mainly due to their biocompatibility, easy molecular vectorization, and good biological half-life. Herein, we report a gold nanoparticle platform as a bimodal imaging probe, capable of coordinating Gd3+ for Magnetic Resonance Imaging (MRI) and 67Ga3+ for Single Photon Emission Computed Tomography (SPECT) imaging. Our AuNPs carry a bombesin analogue with affinity towards the gastrin releasing peptide receptor (GRPr), overexpressed in a variety of human cancer cells, namely PC3 prostate cancer cells. The potential of these multimodal imaging nanoconstructs was thoroughly investigated by the assessment of their magnetic properties, in vitro cellular uptake, biodistribution, and radiosensitisation assays. The relaxometric properties predict a potential T1- and T2- MRI application. The promising in vitro cellular uptake of 67Ga/Gd-based bombesin containing particles was confirmed through biodistribution studies in tumor bearing mice, indicating their integrity and ability to target the GRPr. Radiosensitization studies revealed the therapeutic potential of the nanoparticles. Moreover, the DOTA chelating unit moiety versatility gives a high theranostic potential through the coordination of other therapeutically interesting radiometals. Altogether, our nanoparticles are interesting nanomaterial for theranostic application and as bimodal T1- and T2- MRI / SPECT imaging probes.
Accuracy of Three Impression Materials on the Totally Edentulous Maxilla: In Vitro/In Silico Comparative Analysis
Authors: Zarone Fernando , Ruggiero Gennaro , Di Mauro Maria Irene , Spagnuolo Gianrico , Ferrari Marco , Sorrentino Roberto
Published: January 2020

Background: This study was aimed at comparing the accuracy of impressions of a reference typodont (RT) reproducing a totally edentulous maxilla made with three impression materials: polysulfide, polyether, and polyvinyl-siloxane. Methods: The RT was scanned using a desktop scanner, obtaining a reference scan. Ten impressions for each of the three tested materials were made using a mechanical device with a standardized and consistent modality. A laboratory scanner performed the digitization of each impression. We produced digital models by processing “in reverse” the scans of the physical impressions using a dedicated software, obtaining three groups (n = 10), respectively. The groups were titled: “polysulfide,” “polyvinyl-siloxane,” and “polyether”. The scans in .stl format were imported into Geomagic Control X and then compared to RT to evaluate the accuracy of each scan by calculating trueness and precision in µm. Recorded data were subjected to descriptive statistics. Results: Trueness (arithmetic proximity) values (95%CI) were: polysulfide = 249.9 (121.3–378.5), polyvinyl-siloxane = 216.8 (123.1–310.6), polyether = 291.1 (219.9–362.3). Precision values (95% CI) were: polysulfide = 261.9 (108.8–415), polyvinyl-siloxane = 209.4 (111.9–306.8), polyether = 283 (227.9–338.1). Statistically significant differences were not detected between the means of the experimental groups, both for trueness and precision. Conclusions: The accuracy of the scans obtained from polyvinyl-siloxane, polysulfide, and polyether impressions can be considered comparable in a fully edentulous maxilla.
The Influence of Environmentally Friendly Flame Retardants on the Thermal Stability of Phase Change Polyurethane Foams
Authors: Liu Dong , Hu Anjie
Published: January 2020

To improve thermal insulation, microencapsulated phase change materials (micro-PCMs), expandable graphite (EG), and ammonium polyphosphate (APP) were introduced into polyurethane foam (PUF) to enhance the thermal stability and improve the thermal insulation behavior. The morphology of the PUF and micro-PCM was studied using a scanning electronic microscope (SEM), while the thermophysical properties of the PUF were investigated using a hot disk thermal constants analyzer and differential scanning calorimetry (DSC). The thermal stability of the PUF was investigated by thermogravimetric analysis (TGA), and the gas products volatilized from the PUF were analyzed by thermogravimetric analysis coupled with Fourier transform infrared spectrometry (TGA-FTIR). The results revealed that the thermal conductivities of the PUF were reduced because micro-PCM is effective in absorbing energy, showing that the PUF functions not only as a thermal insulation material but also as a heat sink for energy absorption. Moreover, the EG and APP were found to be effective in improving the thermal stabilities of the PUF, and the optimized formulation among EG, APP, and micro-PCMs in the PUF showed a significant synergistic effect.
Analysis and Characterization on Dynamic Recrystallization in Casting AZ31 Mg Alloys Under Plane Strain Compression
Authors: Xu Li , Xiang Minghua , Wang Jun , Zhang Jun , Wang Chenning , Xie Chao
Published: January 2020

Studies on twinning, twin-induced dynamic recrystallization (TDRX), and their temperature and strain rate dependences are of considerable significance to the ultimate strength and plastic formability of the coarse-grained Mg alloys during severe plastic deformation. Plane strain compression tests were conducted on the parallelepiped samples of casting AZ31 Mg alloys. The twinning and recrystallization behaviors close to and away from the crack boundaries were characterized using electron backscatter diffraction. The results show: (1) with increasing strain rate for tests, the extension twin proliferates significantly. Due to the local stress concentration, the TDRX is more active in the area close to the crack tip and exhibits the positive strain-rate sensitivity as twinning; (2) the TDRX is not only stress-favored but also closely links to the temperature. However, the TDRX is not utterly proportional to the temperature. Compared to 400 °C, 300 °C is more beneficial to the TDRX, achieving the higher strength and plastic deformability. The main reason is that the higher strain-hardening rate and flow stress at the higher strain rate and lower temperature motivates the transformation from twinning to the fine twin-walled grains more efficiently, and the stress-favored TDRX is crucial to refine grains and continue plastic deformation for the casting Mg alloys with coarse grains.
Selectivity of Tungsten Oxide Synthesized by Sol-Gel Method Towards Some Volatile Organic Compounds and Gaseous Materials in a Broad Range of Temperatures
Authors: Ramanavičius Simonas , Petrulevičienė Milda , Juodkazytė Jurga , Grigucevičienė Asta , Ramanavičius Arūnas
Published: January 2020

In this research, the investigation of sensing properties of non-stoichiometric WO3 (WO3−x) film towards some volatile organic compounds (VOC) (namely: Methanol, ethanol, isopropanol, acetone) and ammonia gas are reported. Sensors were tested at several temperatures within the interval ranging from a relatively low temperature of 60 up to 270 °C. Significant variation of selectivity, which depended on the operational temperature of sensor, was observed. Here, the reported WO3/WO3–x-based sensing material opens an avenue for the design of sensors with temperature-dependent sensitivity, which can be applied in the design of new gas- and/or VOC-sensing systems that are dedicated for the determination of particular gas- and/or VOC-based analyte concentration in the mixture of different gases and/or VOCs, using multivariate analysis of variance (MANOVA).
Material Design Methodology for Optimized Wear-Resistant Thermoplastic–Matrix Composites Based on Polyetheretherketone and Polyphenylene Sulfide
Authors: Panin Sergey V. , Lyukshin Boris A. , Bochkareva Svetlana A. , Kornienko Lyudmila A. , Nguyen Duc Ahn , Hiep Le Thi My , Panov Iliya L. , Grishaeva Nataliya Y.
Published: January 2020

The main goal of this paper is to design and justify optimized compositions of thermoplastic–matrix wear-resistant composites based on polyetheretherketone (PEEK) and polyphenylene sulfide (PPS). Their mechanical and tribological properties have been specified in the form of bilateral and unilateral limits. For this purpose, a material design methodology has been developed. It has enabled to determine the optimal degrees of filling of the PEEK- and PPS-based composites with carbon microfibers and polytetrafluoroethylene particles. According to the results of tribological tests, the PEEK-based composites have been less damaged on the metal counterpart than the PPS-based samples having the same degree of filling. Most likely, this was due to more uniform permolecular structure and greater elasticity of the matrix. The described methodology is versatile and can be used to design various composites. Its implementation does not impose any limits on the specified properties of the material matrix or the reinforcing inclusions. The initial data on the operational characteristics can be obtained experimentally or numerically. The methodology enables to design the high-strength wear-resistant composites which are able to efficiently operate both in metal–polymer and ceramic–polymer friction units.
Electromechanical Behaviors of Graphene Reinforced Polymer Composites: A Review
Authors: Feng Chuang , Zhu Dong , Wang Yu , Jin Sujing
Published: January 2020

Graphene (including its derivatives)-reinforced polymer composites (GRPCs) have been drawing tremendous attention from academic and industrial communities for developing smart materials and structures. Such interest stems from the excellent combination of the mechanical and electrical properties of these composites while keeping the beneficial intrinsic attributes of the polymers, including flexibility, easy processability, low cost and good biological and chemical compatibility. The electromechanical performances of these GRPCs are of great importance for the design and optimization of engineering structures and components. Extensive work has been devoted to this topic. This paper reviews the recent studies on the electromechanical behaviors of GRPCs. First the methods and techniques to manufacture graphene and GRPCs are introduced, in which the pros and cons of each method are discussed. Then the experimental examination and theoretical modeling on the electromechanical behaviors of the nanocomposites are presented and discussed.
Surface Analysis of Wire-Electrical-Discharge-Machining-Processed Shape-Memory Alloys
Authors: Chaudhari Rakesh , Vora Jay J. , Patel Vivek , López de Lacalle L. N. , Parikh D. M.
Published: January 2020

Shape-memory alloys such as nitinol are gaining popularity as advanced materials in the aerospace, medical, and automobile sectors. However, nitinol is a difficult-to-cut material because of its versatile specific properties such as the shape-memory effect, superelasticity, high specific strength, high wear and corrosion resistance, and severe strain hardening. Anunconventional machining process like wire-electrical-discharge-machining (WEDM) can be effectively and efficiently used for the machining of such alloys, although the WEDM-induced surface integrity of nitinol hassignificant impact on material performance. Therefore, this work investigated the surface integrity of WEDM-processed nitinol samples using digital microscopy imaging, scanning electron microscopy (SEM), and energy-dispersive X-ray (EDX) analysis. Three-dimensional analysis of the surfaces was carried out in two different patterns (along the periphery and the vertical plane of the machined surface) andrevealed that surface roughness was maximalat the point where the surface was largely exposed to the WEDM dielectric fluid. To attain the desired surface roughness, appropriate discharge energy is required that, in turn, requires the appropriate parameter settings of the WEDM process. Different SEM image analyses showed a reduction in microcracks and pores, and in globule-density size at optimized parameters. EDX analysis revealed the absence of wire material on the machined surface
Fire Resistance of Alkali Activated Geopolymer Foams Produced from Metakaolin and Na2O2
Authors: Peng Xi , Li Han , Shuai Qin , Wang Liancong
Published: January 2020

This work aims to investigate the feasibility that alkali-based geopolymer foams produced from metakaolin and Na2O2 are applied for fire protection. Dry bulk density, porosity, mechanical strength, thermal conductivity, and fire resistance of the geopolymer foams are discussed as a function of the Na2O2 amounts. As Na2O2 content varies from 1% to 4%, dry bulk density, mechanical strength and thermal conductivity of the geopolymer foams approximately exhibit opposite trends with that of the porosity. At the later stage of the 3 h fire-resistance tests, the reverse-side temperatures of all tested samples were always maintained at 220–250 °C. Meanwhile, the amorphous skeleton structures have been converted to smooth ceramics during the high temperature processes, which is the main reason that the geopolymer foams possess a stable porous structure and excellent fire resistance. Therefore, we could conclude that alkali-activated geopolymer foams with extraordinary fire resistance have great potential for fire protection applications.
Constant Sign Solutions to Linear Fractional Integral Problems and Their Applications to the Monotone Method
Authors: Cao Labora Daniel , Rodríguez-López Rosana
Published: January 2020

This manuscript provides some results concerning the sign of solutions for linear fractional integral equations with constant coefficients. This information is later used to prove the existence of solutions to some nonlinear problems, together with underestimates and overestimates. These results are obtained after applying suitable modifications in the classical process of monotone iterative techniques. Finally, we provide an example where we prove the existence of solutions, and we compute some estimates.
Interference among Multiple Vibronic Modes in Two-Dimensional Electronic Spectroscopy
Authors: Farfan Camille A. , Turner Daniel B.
Published: January 2020

Vibronic coupling between electronic and vibrational states in molecules plays a critical role in most photo-induced phenomena. Many key details about a molecule’s vibronic coupling are hidden in linear spectroscopic measurements, and therefore nonlinear optical spectroscopy methods such as two-dimensional electronic spectroscopy (2D ES) have become more broadly adopted. A single vibrational mode of a molecule leads to a Franck–Condon progression of peaks in a 2D spectrum. Each peak oscillates as a function of the waiting time, and Fourier transformation can produce a spectral slice known as a ‘beating map’ at the oscillation frequency. The single vibrational mode produces a characteristic peak structure in the beating map. Studies of single modes have limited utility, however, because most molecules have numerous vibrational modes that couple to the electronic transition. Interactions or interference among the modes may lead to complicated peak patterns in each beating map. Here, we use lineshape-function theory to simulate 2D ES arising from a system having multiple vibrational modes. The simulations reveal that the peaks in each beating map are affected by all of the vibrational modes and therefore do not isolate a single mode, which was anticipated.
Acknowledgement to Reviewers of Microorganismsin 2019
Authors: Microorganisms Editorial Office
Published: January 2020

The editorial team greatly appreciates the reviewers who have dedicated their considerable time and expertise to the journal’s rigorous editorial process over the past 12 months, regardless of whether the papers are finally published or not [...]
A MATE Transporter is Involved in Pathogenicity and IAA Homeostasis in the Hyperplastic Plant Pathogen Pseudomonas savastanoi pv. nerii
Authors: Tegli Stefania , Bini Lorenzo , Calamai Silvia , Cerboneschi Matteo , Biancalani Carola
Published: January 2020

During the last years, many evidences have been accumulating about the phytohormone indole-3-acetic acid (IAA) as a multifaceted compound in the microbial world, with IAA playing a role as a bacterial intra and intercellular signaling molecule or as an effector during pathogenic or beneficial plant–bacteria interactions. However, pretty much nothing is known on the mechanisms that bacteria use to modulate IAA homeostasis, in particular on IAA active transport systems. Here, by an approach combining in silico three-dimensional (3D) structural modeling and docking, mutagenesis, quantitative gene expression analysis, and HPLC FLD auxin quantitative detection, for the first time a bacterial multidrug and toxic compound extrusion (MATE) transporter was demonstrated to be involved in the efflux of IAA, as well as of its conjugate IAA–Lysine, in the plant pathogenic hyperplastic bacterium Pseudomonas savastanoi pv. nerii strain Psn23. Furthermore, according to the role proved to be played by Psn23 MatE in the development of plant disease, and to the presence of Psn23 MatE homologs in all the genomospecies of the P. syringae complex, this membrane transporter could likely represent a promising target for the design of novel and selective anti-infective molecules for plant disease control.
Nitrogen Preferences during Alcoholic Fermentation of Different Non-Saccharomyces Yeasts of Oenological Interest
Authors: Roca-Mesa Helena , Sendra Sonia , Mas Albert , Beltran Gemma , Torija María-Jesús
Published: January 2020

Non-Saccharomyces yeasts have long been considered spoilage microorganisms. Currently, oenological interest in those species is increasing, mostly due to their positive contribution to wine quality. In this work, the fermentative capacity and nitrogen consumption of several non-Saccharomyces wine yeast (Torulaspora delbrueckii, Lachancea thermotolerans, Starmerella bacillaris, Hanseniaspora uvarum, and Metschnikowia pulcherrima) were analyzed. For this purpose, synthetic must with three different nitrogen compositions was used: a mixture of amino acids and ammonium, only organic or inorganic nitrogen. The fermentation kinetics, nitrogen consumption, and yeast growth were measured over time. Our results showed that the good fermentative strains, T. delbrueckii and L. thermotolerans, had high similarities with Saccharomyces cerevisiae in terms of growth, fermentation profile, and nitrogen assimilation preferences, although L. thermotolerans presented an impaired behavior when only amino acids or ammonia were used, being strain-specific. M. pulcherrima was the non-Saccharomyces strain least affected by the nitrogen composition of the medium. The other two poor fermentative strains, H. uvarum and S. bacillaris, behaved similarly regarding amino acid uptake, which occurred earlier than that of the good fermentative species in the absence of ammonia. The results obtained in single non-Saccharomyces fermentations highlighted the importance of controlling nitrogen requirements of the wine yeasts, mainly in sequential fermentations, in order to manage a proper nitrogen supplementation, when needed.
A Case Study of Enhanced Sulfidization Flotation of Lead Oxide Ore: Influence of Depressants
Authors: Xie Haiyun , Sun Rui , Wu Jizong , Feng Dongxia , Gao Likun
Published: January 2020

The refractory lead oxide ore has become an important source of lead metal with the continuous depletion of lead sulfide minerals. Lead oxide ore is of poor floatability and there are few cases to concentrate it successfully. In this study, the sulfidization-xanthate flotation method is applied for the beneficiation of lead oxide ore in Yunnan Province (China) with sodium hexametaphosphate and carboxymethyl cellulose as depressant. Chemical analysis and phase analysis was performed to explore the physicochemical property of raw ore, which provides a research basis for process design and operational control. The main influencing factors during the process, including grinding fineness, reagent types, and dosage, etc., have been studied through flotation tests. Zeta potential measurements and Fourier transfer-infrared spectrometry (FTIR) analysis were conducted to reveal the function mechanism of the reagents. Based on the determined experimental conditions, open circuit tests and closed circuit tests with one stage rougher, three-stage scavenger, and two-stage cleaner flotation, were carried out with the run-of-mine ore with a lead grade of 4.57%. Through close circuit bench test, the lead concentrate with a lead grade of 64.08% and recovery of 92.30% was obtained. This study is of special value, as it provides referencing significance for economically exploiting lead oxide ore.
The Emerging Role of Vitamin C as a Treatment for Sepsis
Authors: Kashiouris Markos G. , L’Heureux Michael , Cable Casey A. , Fisher Bernard J. , Leichtle Stefan W. , Fowler Alpha A.
Published: January 2020

Sepsis, a life-threatening organ dysfunction due to a dysregulated host response to infection, is a leading cause of morbidity and mortality worldwide. Decades of research have failed to identify any specific therapeutic targets outside of antibiotics, infectious source elimination, and supportive care. More recently, vitamin C has emerged as a potential therapeutic agent to treat sepsis. Vitamin C has been shown to be deficient in septic patients and the administration of high dose intravenous as opposed to oral vitamin C leads to markedly improved and elevated serum levels. Its physiologic role in sepsis includes attenuating oxidative stress and inflammation, improving vasopressor synthesis, enhancing immune cell function, improving endovascular function, and epigenetic immunologic modifications. Multiple clinical trials have demonstrated the safety of vitamin C and two recent studies have shown promising data on mortality improvement. Currently, larger randomized controlled studies are underway to validate these findings. With further study, vitamin C may become standard of care for the treatment of sepsis, but given its safety profile, current treatment can be justified with compassionate use.
Starch Digestion Enhances Bioaccessibility of Anti-Inflammatory Polyphenols from Borlotti Beans (Phaseolus vulgaris)
Authors: Perez-Hernandez Lucia Margarita , Nugraheni Kartika , Benohoud Meryem , Sun Wen , Hernández-Álvarez Alan Javier , Morgan Michael R. A. , Boesch Christine , Orfila Caroline
Published: January 2020

The consumption of beans has been associated with chronic disease prevention which may be attributed to the polyphenols present in the seed coat and endosperm. However, their bioaccessibility is likely to be limited by interactions with bean matrix components, including starch, protein and fibre. The aim of this project was to evaluate the effect of domestic processing and enzymatic digestion on the bioaccessibility of polyphenols from Borlotti beans (Phaseolus vulgaris) and to test their anti-inflammatory properties in a macrophage cell model. In vitro digestion of cooked beans released twenty times more polyphenols (40.4 ± 2.5 mg gallic acid equivalents (GAE)/g) than domestic processing (2.22 ± 0.1 mg GAE/g), with starch digestion contributing to the highest release (30.9 ± 0.75 mg GAE/g). Fluorescence microscopy visualization of isolated bean starch suggests that polyphenols are embedded within the granule structure. LC-MS analysis showed that cooked Borlotti bean contain flavonoids, flavones and hydroxycinnamic acids, and cooked bean extracts exerted moderate anti-inflammatory effects by decreasing mRNA levels of IL1β and iNOS by 25% and 40%, respectively. In conclusion, the bioaccessibility of bean polyphenols is strongly enhanced by starch digestion. These polyphenols may contribute to the health benefits associated with bean consumption.
Effect of Maternal Obesity in Mice on IL-6 Levels and Placental Endothelial Cell Homeostasis
Authors: Kretschmer Tobias , Schulze-Edinghausen Merle , Turnwald Eva-Maria , Janoschek Ruth , Bae-Gartz Inga , Zentis Peter , Handwerk Marion , Wohlfarth Maria , Schauss Astrid , Hucklenbruch-Rother Eva , Dötsch Jörg , Appel Sarah
Published: January 2020

Obesity during pregnancy is a known health risk for mother and child. Since obesity is associated with increased inflammatory markers, our objectives were to determine interleukin-6 (IL-6) levels in obese mice and to examine the effect of IL-6 on placental endothelial cells. Placentas, blood, and adipose tissue of C57BL/6N mice, kept on high fat diet before and during pregnancy, were harvested at E15.5. Serum IL-6 levels were determined and endothelial cell markers and IL-6 expression were measured by qRT-PCR and western blot. Immunostaining was used to determine surface and length densities of fetal capillary profiles and placental endothelial cell homeostasis. Human placental vein endothelial cells were cultured and subjected to proliferation, apoptosis, senescence, and tube formation assays after stimulation with hyperIL-6. Placental endothelial cell markers were downregulated and the percentage of senescent endothelial cells was higher in the placental exchange zone of obese dams and placental vascularization was strongly reduced. Additionally, maternal IL-6 serum levels and IL-6 protein levels in adipose tissue were increased. Stimulation with hyperIL-6 provoked a dose dependent increase of senescence in cultured endothelial cells without any effects on proliferation or apoptosis. Diet-induced maternal obesity led to an IUGR phenotype accompanied by increased maternal IL-6 serum levels. In the placenta of obese dams, this may result in a disturbed endothelial cell homeostasis and impaired fetal vasculature. Cell culture experiments confirmed that IL-6 is capable of inducing endothelial cell senescence.
Expression Patterns of Muscle-Specific miR-133b and miR-206 Correlate with Nutritional Status and Sarcopenia
Authors: Iannone Francesca , Montesanto Alberto , Cione Erika , Crocco Paolina , Caroleo Maria Cristina , Dato Serena , Rose Giuseppina , Passarino Giuseppe
Published: January 2020

Sarcopenia and malnutrition are commonly occurring conditions in the elderly that frequently coexist, leading to substantial effects on morbidity/mortality. Evidence established muscle-specific microRNAs (miRNAs) or myomiRs as essential regulators of skeletal muscle processes, from myogenesis to muscle homeostasis. This study aimed to evaluate the association between myomiRs and sarcopenia and explore the potential of nutrition in mediating this association. qPCR was employed to characterize the myomiR-1, -133a/b, -206, -208b, and -499 expression profiles of 109 non-sarcopenic and 109 sarcopenic subjects. In our sample, the proportion malnourished or at-risk subjects was higher in sarcopenia (p < 0.001). Among the detected myomiRs (miR-133a/b and miR-206), lower levels of miR-133b was significantly associated with the presence of sarcopenia (p = 0.006); however, this relationship was not independent from nutritional status in multivariate analysis, suggesting a mediating effect of nutrition on the relationship between miR-133b and sarcopenia. Correlation analyses showed that lower miR-133b levels were associated with poor nutritional status (Mini Nutritional Assessment Long Form (MNA-LF) score, p = 0.005); furthermore, correlations with albumin, ferritin, and iron were found. Similar results were obtained for miR-206. Statistically more significant correlations were observed in subjects with sarcopenia. In conclusion, our findings highlight a nutrient-miR-133b/miR-206 pathway having a potential role in the age-related muscle decline.
Health Benefits of Whey or Colostrum Supplementation in Adults ≥35 Years; a Systematic Review
Authors: Blair Merran , Kellow Nicole J. , Dordevic Aimee L. , Evans Stephanie , Caissutti Julia , McCaffrey Tracy A.
Published: January 2020

Food-health claims are an important method of translating nutrition research to consumers. Whey and colostrum are thought to exert health benefits to adults, but it is unclear what measurable, objective health benefits they impart. This review aimed to identify the objective health benefits of bovine whey or colostrum-based beverages to healthy adults aged ≥35 years to substantiate a food-health claim. Seven databases were systematically searched. Eligible articles were RCTs that involved healthy adults aged ≥35 years, consuming whey or colostrum in beverage form and measuring objective health markers. Quality assessment and data extraction was conducted in duplicate. The searches identified 9943 papers and 16 were included in this review; 13 studies, reported across 15 papers, related to whey, one study to colostrum. The outcomes identified were body composition, bone mineral density, biochemical markers, such as blood glucose and lipids, and muscle strength and synthesis. Heterogeneous outcomes, high risk of bias and inconsistent findings resulted in inconclusive evidence to substantiate a food-health claim. Clearer reporting and consensus on a minimum set of objective measures would allow for more robust recommendations regarding food-health claims. Protecting consumers from misleading health claims will require collaboration between regulators, researchers, and the food industry.