bluuebunny/crossref_metadata_2025 huggingface dataset:
Social support for overcoming fear of contagion at the start of the COVID-19 pandemic. Cross-sectional study in Latin America
Authors: Carrillo Irene , Poblete Rodrigo , Serpa Piedad , Martin-Delgado Jimmy , Giménez Alejandro , Mira José Joaquín
Published: September 2023

Background

The psychological impact of the COVID-19 pandemic on healthcare professionals has been widely studied, along with different strategies to minimize it. However, professionals’ assessment of the social support received and the factors that mitigated their fear of contagion have not been described. This study aimed to assess healthcare professionals’ satisfaction with the social support and information received and their efforts to self-isolate to avoid infecting loved ones in Chile, Colombia, and Ecuador.

Methods

A cross-sectional online survey, conducted from July to September 2020 in three Latin American countries, elicited healthcare professionals’ satisfaction with social support from colleagues, their community, the media, and scientific societies; as well as the information received about the evolution of the pandemic and measures to avoid contagion. The EASE scale was used to measure acute stress.

Results

Survey responses were received from 700 professionals. The response rate per country exceeded the estimated sample size except in the case of Colombia, which was 81.4%. In general, peer support was highly valued, though satisfaction was lower in high-risk units (p < 0.001). Those who directly assisted COVID-19 patients perceived the least community support (p = 0.023). Professionals from high-risk units (p = 0.013) and those who experienced greater acute stress (p = 0.05) assigned the lowest rating to the information offered by the centre on the pandemic. Men perceived more support from colleagues and better information from the centre than women (p < 0.05). Just 10.7% of professionals changed their residence during the pandemic, but those who worked in high-risk areas self-isolated more frequently (p = 0.026).

Conclusion

In the early stages of the COVID-19 pandemic, healthcare professionals in Chile, Colombia, and Ecuador greatly valued the support received from their peers. Being infected with COVID-19, working in high-risk areas, experiencing higher self-reported acute stress, and having an infected co-worker were predictors for self-isolation to protect their relatives. These results point to the appropriateness of putting in place institutional resources based on peer support and specific communication strategies and action protocols to build resilience and responsiveness to future health crises.
Validity and reliability of the Korean version of the gender-friendliness barriers in nursing programs scale
Authors: Park Seon-Min , Kim Jung-Hee
Published: September 2023

Introduction

The gender-friendliness barriers in nursing programs (GFB-NP) were used to measure perceived gender affinity among male nursing students in nursing education programs. Originally developed in Taiwan, this scale has not been used in Korea. The purpose of this study is to confirm the reliability and validity of the GFB-NP scale for Korean male nursing students.

Methods

A convenience sample of male nursing students enrolled in the 1st to 4th year of nursing departments at five four-year universities located in three cities in Korea was used in the study. To confirm the validity and factor structure of the scale, both exploratory factor analysis and confirmatory factor analysis were employed.

Results

The results support a four-factor structure: Professional acquisition opportunity, peer interaction, sociocultural prejudice, and gender role attitude. We confirmed that the Korean version of the GFB-NP is an appropriate tool for measuring the gender-friendliness educational environment perceived by male nursing students in nursing education.

Discussion

The GFB-NP will serve as a framework for developing counseling and management strategies to help male nursing students successfully adapt to school life within the nursing education curriculum. Research with a longitudinal study design is recommended to investigate the progression of school adaptation through undergraduate program courses.
The effect of intranasal oxytocin on social reward processing in humans: a systematic review
Authors: Kraus Jakub , Výborová Eliška , Silani Giorgia
Published: September 2023

Understanding the neurobiology of social reward processing is fundamental, holding promises for reducing maladaptive/dysfunctional social behaviors and boosting the benefits associated with a healthy social life. Current research shows that processing of social (vs. non-social) rewards may be driven by oxytocinergic signaling. However, studies in humans often led to mixed results. This review aimed to systematically summarize available experimental results that assessed the modulation of social reward processing by intranasal oxytocin (IN-OXY) administration in humans. The literature search yielded 385 results, of which 19 studies were included in the qualitative synthesis. The effects of IN-OXY on subjective, behavioral, and (neuro)physiological output variables are discussed in relation to moderating variables—reward phase, reward type, onset and dosage, participants’ sex/gender, and clinical condition. Results indicate that IN-OXY is mostly effective during the consumption (“liking”) of social rewards. These effects are likely exerted by modulating the activity of the prefrontal cortex, insula, precuneus, anterior cingulate cortex, amygdala, and striatum. Finally, we provide suggestions for designing future oxytocin studies.

Systematic review registration

https://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42021278945, identifier CRD42021278945.
COVID-19 healthcare and social-related needs from the perspective of Spanish patients and healthcare providers: a qualitative analysis of responses to open-ended questions
Authors: Duarte-Díaz Andrea , Aparicio Betancourt Mariana , Seils Laura , Orrego Carola , Perestelo-Pérez Lilisbeth , Barrio-Cortes Jaime , Beca-Martínez María Teresa , Bermejo-Caja Carlos Jesús , González-González Ana Isabel
Published: September 2023

Introduction

Identifying stakeholders’ needs is crucial to informing decisions and policy development. This study aims to identify healthcare and social-related needs and effective strategies associated with COVID-19 from the first-person perspectives of patients and healthcare providers.

Methods

Cross-sectional online survey design including qualitative open-ended questions, conducted in primary care and hospital settings across Spain, with 12 out of 19 regions represented. Adults aged 18 years and older, who (a) resided in Spain and had a history of COVID-19 or (b) worked as healthcare providers delivering direct or indirect care for people with COVID-19 in Spanish primary care or hospitals during 2020 were eligible to participate. Recruitment was conducted via social-media networks (Twitter, LinkedIn, and WhatsApp) and communication channels of key organizations including patient and professional associations and groups. A total of 182 people were invited to complete the surveys and 76 people completed the surveys (71% women), of which 33 were home-isolated patients, 14 were hospitalized patients, 16 were primary care professionals, and 13 were hospital care professionals.

Results

A total of 327 needs and 86 effective strategies and positive aspects were identified across surveys and classified into the following overarching themes: (i) Accessibility, (ii) Basic needs, (iii) Clinical care, (iv) Person-and-family centered care, (v) Caring for the healthcare professional, (vi) Protocolization, information, health campaigns, and education, (vii) Resource availability, (viii) and Organizational needs/strategies.

Discussion

Findings indicate the Spanish health and social care systems were generally unprepared to combat COVID-19. Implications for research, practice, and policy focus on integrating first-person perspectives as best practice to identify, prioritize and address needs to increase health and social care systems capacity and preparedness, as well as providing well-co-coordinated responses across government, healthcare, and non-government sectors to promote and protect the physical and mental health of all.
A study on the path of governance in health insurance fraud considering moral hazard
Authors: Liu Jusheng , Wang Yuan , Yu Jiali
Published: September 2023

Combating health insurance fraud is of utmost importance to physicians, patients, and health insurers. To delve into the mechanisms of health insurance fraud between doctors and patients, this study employed evolutionary game theory to construct a model that comprehensively considers moral hazard, fraud cost, reward, punishment, bribes from patients, and other factors. Through theoretical analysis and numerical simulation of the model, the study discovered that the evolution of governance behavior in health insurance fraud is closely linked to its initial construction of the payment matrix and the initial selection of parameters for the payment matrix. Additionally, increasing penalties for fraudulent behavior, increasing the cost of fraud for both doctors and patients, and reducing moral hazard for both can effectively drive the final strategy of the system toward a non-fraudulent state. The study aims to provide valuable insights and recommendations to doctors, patients, and medical insurance institutions in establishing a sound governance environment for managing fraud behavior in health insurance.
Presenteeism and mental health of workers during the COVID-19 pandemic: a systematic review
Authors: García-Iglesias Juan Jesús , Gómez-Salgado Juan , Apostolo Joao , Rodrigues Rogério , Costa Emília Isabel , Ruiz-Frutos Carlos , Martínez-Isasi Santiago , Fernández-García Daniel , Vilches-Arenas Ángel
Published: September 2023

Background

A large number of workers attend work despite being ill. Attending work during sickness can have a number of consequences for the worker (e.g., worsening of physical and mental condition), for co-workers, and for the company, and for service users.

Objectives

The aim of this study was to assess the factors influencing presenteeism and mental health of workers during the COVID-19 pandemic.

Methods

A systematic review following the PRISMA format was conducted in the PubMed, Scopus, Web of Science (WoS), Cumulative Index to Nursing and Allied Health Literature (CINAHL), PsycInfo, and ScienceDirect electronic databases in January 2023, using the following key words: Presenteeism, Mental Health, and COVID-19. The eligibility criteria applied were original articles published in English, Spanish, French, German, and Portuguese, workers during the COVID-19 pandemic (data collection date: January 01, 2020 – January 01, 2023), and articles assessing at least one measure of presenteeism and mental health status. Methodological quality was assessed using the critical appraisal tools of the Joanna Briggs Institute. The followed protocol is listed in the International Prospective Register of Systematic Reviews (PROSPERO) with code CRD42023391409.

Results

A total of 25 studies were included in this review recruiting a total of 164,274 participants. A number of factors influencing mental health and sickness presenteeism were identified: (1) mental health-related factors (burnout [in 4 studies], stress [in 9 studies], depression [in 1 study], fear of COVID-19 [in 1 study], no well-being [in 2 studies], etc.); (2) individual factors (health status [in 1 study], being young [in 1 study], workers who experienced interrupted medical care [in 2 studies], having a chronic disease [in 1 study], etc.); (3) factors related to the situation caused by COVID-19 (confinement, symptoms, loss of contract, risk of bankruptcy, etc. [in 1 study each one]); and (4) factors derived from working conditions (organisational support [in 1 study], patient care [in 1 study], work functioning or task performance impairment [in 4 studies], work fatigue [in 2 studies], safety climate [in 1 study], workload [in 1 study], etc.).

Conclusion

Identifying the key determinants of presenteeism and understanding the phenomena and origins of sickness presenteeism will help to create a safe working environment and optimal organisational systems to protect vulnerable workers in a pandemic context.

Systematic review registration

The unique identifier is CRD42023391409.
Probing the compositional and rheological properties of gastropod locomotive mucus
Authors: Barajas-Ledesma Edgar , Holland Chris
Published: September 2023

Gastropods, such as snails and slugs, can excrete mucus to aid in movement and adhesion. However, very few studies have examined the physical relationship between mucus composition and function. Here, we explore the role of mucus polymers (specifically their proteins) and their influence on the material properties of locomotive mucus. Using a range of spectroscopic, thermal, and rheological analytical tools, we characterised locomotive mucus from six gastropod species across four families. We report that all mucus tested consisted of 97%–99% water, and the remaining 1%–3% solid content contained a range of proteins (41–377 kDa, 18 of which are previously undocumented), which we propose contribute to its weak gel behaviour (1.58–36.33 Pa•s at 1 rad/s). Our results indicate that mucus properties are also grouped at the family level, suggesting that niche-specific adaptation occurs in these materials. We expect our study to offer a broader approach to how a correlation between properties is crucial for understanding the stability and functionality of snail mucus.
The contribution of remote sensing and input feature selection for groundwater level prediction using LSTM neural networks in the Oum Er-Rbia Basin, Morocco
Authors: Bouramtane Tarik , Leblanc Marc , Kacimi Ilias , Ouatiki Hamza , Boudhar Abdelghani
Published: September 2023

The planning and management of groundwater in the absence of in situ climate data is a delicate task, particularly in arid regions where this resource is crucial for drinking water supplies and irrigation. Here the motivation is to evaluate the role of remote sensing data and Input feature selection method in the Long Short Term Memory (LSTM) neural network for predicting groundwater levels of five wells located in different hydrogeological contexts across the Oum Er-Rbia Basin (OER) in Morocco: irrigated plain, floodplain and low plateau area. As input descriptive variable, four remote sensing variables were used: the Integrated Multi-satellite Retrievals (IMERGE) Global Precipitation Measurement (GPM) precipitation, Moderate resolution Imaging Spectroradiometer (MODIS) normalized difference vegetation index (NDVI), MODIS land surface temperature (LST), and MODIS evapotranspiration. Three LSTM models were developed, rigorously analyzed and compared. The LSTM-XGB-GS model, was optimized using the GridsearchCV method, and uses a single remote sensing variable identified by the input feature selection method XGBoost. Another optimized LSTM model was also constructed, but uses the four remote sensing variables as input (LSTM-GS). Additionally, a standalone LSTM model was established and also incorporating the four variables as inputs. Scatter plots, violin plots, Taylor diagram and three evaluation indices were used to verify the performance of the three models. The overall result showed that the LSTM-XGB-GS model was the most successful, consistently outperforming both the LSTM-GS model and the standalone LSTM model. Its remarkable accuracy is reflected in high R2 values (0.95 to 0.99 during training, 0.72 to 0.99 during testing) and the lowest RMSE values (0.03 to 0.68 m during training, 0.02 to 0.58 m during testing) and MAE values (0.02 to 0.66 m during training, 0.02 to 0.58 m during testing). The LSTM-XGB-GS model reveals how hydrodynamics, climate, and land-use influence groundwater predictions, emphasizing correlations like irrigated land-temperature link and floodplain-NDVI-evapotranspiration interaction for improved predictions. Finally, this study demonstrates the great support that remote sensing data can provide for groundwater prediction using ANN models in conditions where in situ data are lacking.
Spatial and temporal correlations of thermospheric zonal winds from GOCE satellite observations
Authors: Molina Ivana , Scherliess Ludger
Published: September 2023

Winds in the thermosphere play an important role in the transport of momentum and energy in the upper atmosphere and affect the composition, dynamics and morphology of the ionospheric plasma. Although the general morphology of the winds is well understood, we are only starting to understand its variability. During the last decade it has become inherently clear that in addition to solar forcing of the thermosphere, the lower atmosphere also is an important driver of thermospheric variability. Therefore, an understanding of thermospheric variability and its spatial and temporal correlations is critical for an improved understanding of the coupled ionosphere-thermosphere system and the coupling to the lower atmosphere. The Gravity Field and Steady-State Ocean Explorer (GOCE) provided zonal winds near dawn and dusk at an altitude of around 260 km from November 2009 to October 2013. We have used GOCE zonal wind observations from low- to mid-latitudes obtained during geomagnetically quiet times to investigate spatial and temporal correlations in the zonal winds near dawn and dusk. Latitudinal correlations were calculated for the GOCE zonal winds for December solstice separately for each year from 2009 to 2012 and their year-to-year variation was established. Correlations between hemispheric conjugate points were found at mid latitudes during the latter years. Latitudinal correlations for December solstice 2009 and June solstice 2010 were compared and the correlation length was found to be consistently larger in the winter hemisphere during dawn and in the summer hemisphere during dusk. Zonal wind longitudinal/temporal correlations were also determined for December 2009 and 2011 and for June 2010 and found to be periodic in longitude/time. The temporal evolution of the temporal/longitudinal correlations were found to gradually decrease over the course of several days. The maxima in the correlation coefficients were always located in the winter hemisphere during dawn and in the summer hemisphere during dusk. During dawn, the largest contributors to the temporal/longitudinal correlations were found to be nonmigrating tides, whereas during dusk, additional waves appear to play important roles.
Lymphocytic hypophysitis in dogs infected with Leishmania spp.
Authors: Frigerio Edenilson Doná , Guizelini Cecilia de Castro , Jussiani Giulia Gonçalves , Março Karen Santos , de Melo Guilherme Dias , Watanabe Tatiane Terumi Negrão , Machado Gisele Fabrino
Published: September 2023

Background

Morphological involvement of endocrine glands, such as the pituitary gland, remain uninvestigated in dogs with canine visceral leishmaniasis. Therefore, this study investigated the presence of amastigotes of Leishmania spp. and characterized inflammatory changes, highlighting the involvement of TCD3+ lymphocytes in different regions of the pituitary gland of dogs.

Methods

Samples were collected from 21 naturally infected dogs and 5 control, uninfected dogs. The different pituitary regions were analyzed in histological sections stained with hematoxylin and eosin (HE) under light microscopy. Inflammation was classified by intensity in a score from 0 to 3, absent (0), mild (1), moderate (2), and marked (3). The immunohistochemical (IHC) evaluation was performed in five high-power fields (hot spot) in a 40x objective of each region with manual counting (Image J1.52ª) of the TCD3+ lymphocytes and for amastigotes analyzed in 40x and 100x objectives. The Shapiro–Wilk test was used to assess the normality of the data. Differences between groups were determined by the Mann Whitney test. The correlation between variables was assessed by Sperman’s correlation test. p < 0.05 were considered statistically significant.

Results

Amastigotes from the pituitary glands of two infected dogs were identified using IHC. The histopathological evaluation stained with hematoxylin and eosin showed greater intensity of inflammation in the pars distalis and pars intermedia regions of infected dogs. IHC for TCD3+ lymphocytes showed a higher median number of immunolabeled cells in pars nervosa in the infected group than in the control group (p < 0.05); and expecting a variation in the distribution and number of these cells in naturally infected dogs, the median of the control group was considered a cut-off point, an increase in T lymphocytes (p < 0.05) was also observed in the pars intermedia and pars distalis of an infected subgroup (n = 10). A moderate significant correlation between the intensity of inflammation and the number of immunolabeled TCD3+ lymphocytes was established in the analyzed pituitary regions, characterizing the occurrence of hypophysitis.

Conclusion

These findings presuppose that inflammation and/or the parasite in the pituitary region can result in gland dysfunction, worsening the clinical condition of the patient and compromising the efficiency of treatment and prognosis.
Genetic identification of Trichinella species found in wild carnivores from the territory of Kazakhstan
Authors: Uakhit Rabiga , Mayer-Scholl Anne , Shin Chincher , Smagulova Ainura , Lider Lyudmila , Leontyev Sergey , Kiyan Vladimir
Published: September 2023

Trichinellosis, also called trichinosis, is a foodborne parasitic disease caused by eating raw or undercooked meat from animals infected with Trichinella spp. larvae and affects both animals and humans. Although on the territory of Kazakhstan, the species characteristics and prevalence of this helminth were studied back in the 90s, the data have not been updated since then. Given the above, our study was aimed at identifying Trichinella spp. using parasitological and molecular genetics methods. In our work, we studied 160 samples of muscle tissue of wild animals living in the natural zones of steppes and semi-deserts. Of the animals examined, 32 were positive for Trichinella spp., including 1 lynx (Lynx lynx), 17 wolves (Canis lupus), 11 foxes (Vulpes vulpes), 1 jackal (Canis aureus) and 2 corsac foxes (Vulpes corsac). Helminths were extracted using the digestion method. DNA was extracted using a Gene Jet commercial kit (Thermo Fisher Scientific, United Kingdom). For species identification a multiplex PCR, amplification of ESV, ITS1, and ITS2 genes regions was performed. After that, uniplex PCR was performed on the 5S rDNA and ITS1 genes region for sequencing analysis. The resulting sequences were subsequently used to construct a phylogenetic tree and the studied samples were identified as Trichinella nativa and Trichinella britovi. Thus, we can conclude that there is a circulation of two species of Trichinella in Kazakhstan, highlighting that constant control and monitoring of wild animals are necessary to prevent transmission and protect the health of people.
Liver Inclusion Appears to Be Protective Against Graft Loss-Due-to Chronic But Not Acute Rejection Following Intestinal Transplantation
Authors: Vianna Rodrigo , Gaynor Jeffrey J. , Selvaggi Gennaro , Farag Ahmed , Garcia Jennifer , Tekin Akin , Tabbara Marina M. , Ciancio Gaetano
Published: September 2023

In intestinal transplantation, while other centers have shown that liver-including allografts have significantly more favorable graft survival and graft loss-due-to chronic rejection (CHR) rates, our center has consistently shown that modified multivisceral (MMV) and full multivisceral (MV) allografts have significantly more favorable acute cellular rejection (ACR) and severe ACR rates compared with isolated intestine (I) and liver-intestine (LI) allografts. In the attempt to resolve this apparent discrepancy, we performed stepwise Cox multivariable analyses of the hazard rates of developing graft loss-due-to acute rejection (AR) vs. CHR among 350 consecutive intestinal transplants at our center with long-term follow-up (median: 13.5 years post-transplant). Observed percentages developing graft loss-due-to AR and CHR were 14.3% (50/350) and 6.6% (23/350), respectively. Only one baseline variable was selected into the Cox model indicating a significantly lower hazard rate of developing graft loss-due-to AR: Transplant Type MMV or MV (p < 0.000001). Conversely, two baseline variables were selected into the Cox model indicating a significantly lower hazard rate of developing graft loss-due-to CHR: Received Donor Liver (LI or MV) (p = 0.002) and Received Induction (p = 0.007). In summary, while MMV/MV transplants (who receive extensive native lymphoid tissue removal) offered protection against graft loss-due-to AR, liver-containing grafts appeared to offer protection against graft loss-due-to CHR, supporting the results of other studies.
Targeting MET Amplification: Opportunities and Obstacles in Therapeutic Approaches
Authors: Kumaki Yuichi , Oda Goshi , Ikeda Sadakatsu
Published: September 2023

The MET gene plays a vital role in cellular proliferation, earning it recognition as a principal oncogene. Therapies that target MET amplification have demonstrated promising results both in preclinical models and in specific clinical cases. A significant obstacle to these therapies is the ability to distinguish between focal amplification and polysomy, a task for which simple MET copy number measurement proves insufficient. To effectively differentiate between the two, it is crucial to utilize comparative measures, including in situ hybridization (ISH) with the centromere or next generation sequencing (NGS) with adjacent genes. Despite the promising potential of MET amplification treatment, the judicious selection of patients is paramount to maximize therapeutic efficacy. The effectiveness of MET inhibitors can fluctuate depending on the extent of MET amplification. Future research must seek to establish the ideal threshold value for MET amplification, identify the most efficacious combination therapies, and innovate new targeted treatments for patients exhibiting MET amplification.
Enhancing Heat Transfer Performance in Simulated Fischer–Tropsch Fluidized Bed Reactor through Tubes Ends Modifications
Authors: Sabri Laith S. , Sultan Abbas J. , Ali Jamal M. , Majdi Hasan Shakir , Al-Dahhan Muthanna H.
Published: September 2023

Fluidized bed reactors are essential in a wide range of industrial applications, encompassing processes such as Fischer–Tropsch synthesis and catalytic cracking. The optimization of performance and reduction in energy consumption in these reactors necessitate the use of efficient heat transfer mechanisms. The present work examines the considerable impact of tube end geometries, superficial gas velocity, and radial position on heat transfer coefficients within fluidized bed reactors. It was found that the tapered tube end configurations have been empirically proven to improve energy efficiency in fluidized bed reactors significantly. For example, at a superficial gas velocity of 0.4 m/s, the tapered end form’s local heat transfer coefficient (LHTC) demonstrated a significant 20% enhancement compared to the flat end shape. The results and findings of this work make a valuable contribution to the advancement of complex models, enhance the efficiency of fluidized bed reactor processes, and encourage further investigation into novel tube geometries.
Thermal Cycling, Microstructure, and Mechanical Properties of Al-Mg-Si-Cu Alloy Bobbin Tool Friction Stir Welded Joints Based on Thermal Index
Authors: Li Yi , Zhou Zhigang , Yin Li , Fu Dingyao , Jiang Haiyi , Yang Yunxin , Lu Jie , Jin Fuming
Published: September 2023

The two main process parameters of Bobbin tool friction stir welding (BT-FSW) are ω (rotational speed) and v (traverse speed). Both of these factors have a significant effect on heat input, microstructure, and mechanical properties. At present, most studies on friction stir welding adopt the control variable method to study the thermal cycling during the welding process and the mechanical properties of joints, and there are few studies on changing the two process parameters at the same time, because it can be difficult to assess the correlation between heat input and mechanical properties when changing both factors at the same time. In this study, the w/v ratio is defined as the thermal index, which is a characteristic value of heat input. The study uses ABAQUS 6.5 software to establish a BT-FSW CEL (coupled Eulerian–Lagrangian) thermal coupling model. This model explores the relationship between joint thermal cycles, microstructure, and mechanical properties for different w and v values with the same w/v ratio. The results show that increasing rotational and traverse speeds under the same w/v ratio leads to an increase in the peak temperature of the nugget zone (NZ). However, the peak temperature of the thermo-mechanically affected zone (TMAZ) and heat-affected zone (HAZ) remained almost constant. Joint strength was highest at a rotational speed of 750 r/min and a traverse speed of 650 mm/min, with a yield strength of 227 MPa. As rotational and traverse speeds increased, the recrystallized grain content of the NZ showed an increasing trend followed by a decreasing trend. The recrystallized grain content of the advancing side thermo-mechanically affected zone (AS-TMAZ) and retreating side thermo-mechanically affected zone (RS-TMAZ) showed a decreasing trend. Joint hardness had a “W” shaped distribution, with the highest average hardness value found in the NZ.
Using the Intrinsic Geometry of Binodal Curves to Simplify the Computation of Ternary Liquid–Liquid Phase Diagrams
Authors: Shcherbakova Nataliya , Gerbaud Vincent , Roger Kevin
Published: September 2023

Phase diagrams are powerful tools to understand the multi-scale behaviour of complex systems. Yet, their determination requires in practice both experiments and computations, which quickly becomes a daunting task. Here, we propose a geometrical approach to simplify the numerical computation of liquid–liquid ternary phase diagrams. We show that using the intrinsic geometry of the binodal curve, it is possible to formulate the problem as a simple set of ordinary differential equations in an extended 4D space. Consequently, if the thermodynamic potential, such as Gibbs free energy, is known from an experimental data set, the whole phase diagram, including the spinodal curve, can be easily computed. We showcase this approach on four ternary liquid–liquid diagrams, with different topological properties, using a modified Flory–Huggins model. We demonstrate that our method leads to similar or better results comparing those obtained with other methods, but with a much simpler procedure. Acknowledging and using the intrinsic geometry of phase diagrams thus appears as a promising way to further develop the computation of multiphase diagrams.
Customers’ Satisfaction of E-Banking in Bangladesh: Do Service Quality and Customers’ Experiences Matter?
Authors: Bashir Md. Abdul , Haque Md. Alaul , Salamzadeh Aidin , Rahman Md. Mizanur
Published: September 2023

The banking sectors are optimistic that electronic banking (E-banking) will help them provide better customer service and strengthen customer relationships. Despite this, a relatively low priority has been given to the level of satisfaction that E-banking users in Bangladesh have regarding the quality of the services they receive and their overall experiences. Consequently, this study aims to determine the effect of service quality and customer experiences on the level of satisfaction perceived by E-banking customers in Bangladesh. Using a convenience sampling technique and a self-administered questionnaire, we gathered data from 315 E-banking customers. The independent variable (service quality and customer experience) and dependent variable (customer satisfaction) on a five-point “Likert-Type Scale” explain the degree to which participants agree or disagree with the questionnaire’s statements. Covariance-based structural equation modeling (CB-SEM) was utilised to analyse the gathered data. The findings of this study indicate that service quality and customer experience significantly positively affect E-banking customer satisfaction in Bangladesh. The outcomes of this study will urge the banking authorities to prioritize service quality to boost customer satisfaction by suggesting several steps to improve the efficiency, effectiveness, and security of the E-banking system.
On the Generalization of Ostrowski-Type Integral Inequalities via Fractional Integral Operators with Application to Error Bounds
Authors: Rahman Gauhar , Vivas-Cortez Miguel , Yildiz Çetin , Samraiz Muhammad , Mubeen Shahid , Yassen Mansour F.
Published: September 2023

The Ostrowski inequality expresses bounds on the deviation of a function from its integral mean. The Ostrowski’s type inequality is frequently used to investigate errors in numerical quadrature rules and computations. In this work, Ostrowski-type inequality is demonstrated using the generalized fractional integral operators. From an application perspective, we present the bounds of the fractional Hadamard inequalities. The results that are being presented involve a number of fractional inequalities that are already known and have been published.
Comprehensive Identification of the Pum Gene Family and Its Involvement in Kernel Development in Maize
Authors: Feng Wenqi , Zhang Hongwanjun , Cao Yang , Yang Cheng , Khalid Muhammad Hayder Bin , Yang Qingqing , Li Wanchen , Wang Yingge , Fu Fengling , Yu Haoqiang
Published: September 2023

The Pumilio (Pum) RNA-binding protein family regulates post-transcription and plays crucial roles in stress response and growth. However, little is known about Pum in plants. In this study, a total of 19 ZmPum genes were identified and classified into two groups in maize. Although each ZmPum contains the conserved Pum domain, the ZmPum members show diversity in the gene and protein architectures, physicochemical properties, chromosomal location, collinearity, cis-elements, and expression patterns. The typical ZmPum proteins have eight α-helices repeats, except for ZmPum2, 3, 5, 7, and 14, which have fewer α-helices. Moreover, we examined the expression profiles of ZmPum genes and found their involvement in kernel development. Except for ZmPum2, ZmPum genes are expressed in maize embryos, endosperms, or whole seeds. Notably, ZmPum4, 7, and 13 exhibited dramatically high expression levels during seed development. The study not only contributes valuable information for further validating the functions of ZmPum genes but also provides insights for improvement and enhancing maize yield.
Developmental Instability and Gene Dysregulation in an Extracted Tetraploid from Hexaploid Wheat
Authors: Li Yang , Sha Yan , Wang Han , Lv Ruili , Zhang Deshi , Li Shuhang , Xu Chunming , Liu Bao
Published: September 2023

The BBAA subgenomes of hexaploid common wheat can be ‘extracted’ to constitute a viable and self-reproducing novel tetraploid wheat, termed extracted tetraploid wheat (ETW). Prior studies have shown ETW manifesting phenotypic abnormalities and alteration in gene expression and epigenetic modifications. No population level investigation has been conducted, leaving the issue unclear regarding whether developmental stability, an essential property evolved in all natural organisms, might have been undermined in ETW. Here, we measured variations in five morphological traits and somatic chromosomal stability in populations of ETW and of its hexaploid donor, a resynthesized hexaploid and a natural tetraploid wheat. We observed phenotypic defects in ETW. Meanwhile, we documented much greater within-population variations in ETW than in the other wheat genotypes, most probably due to disrupted developmental stability in ETW. Also, somatic structural chromosome variations were detected only in ETW. Comparative transcriptome analyses indicated that the disrupted developmental stability of ETW is likely linked to massive dysregulation of genome-wide gene expression rather than to genetic mutations. Population network analysis of gene expression implicated intrinsic connectivity among the variable traits, while gene set enrichment analysis provided possible links between dysregulated gene expression and interlaced trait variation.
Novel Combination of Choline with Withania somnifera (L.) Dunal, and Bacopa monnieri (L.) Wetts Reduced Oxidative Stress in Microglia Cells, Promoting Neuroprotection
Authors: Borgonetti Vittoria , Galeotti Nicoletta
Published: September 2023

Memory deficit is one of the major negative outcomes of chronic stress. Cholinergic system modulates memory not only through the neuronal cells, but also via interactions with non-neuronal cells, suggesting that microglia can influence synaptic function and plasticity, contributing to cognition and memory function. Withania somnifera (L.) Dunal (WS) and Bacopa monnieri (L.) Wettst (BM), are traditional herbal medicinal products used for the temporary relief of symptoms of stress. The aim of this study was to investigate whether choline (CLN) activity could be enhanced via an association with adaptogens: WS and BM extracts. First, we optimized an in vitro model of corticotropin-releasing hormone (CRH)-induced oxidative stress on microglial BV2 cells. CRH 100 nM reduced BV2 cell viability and induced morphological changes and neurotoxicity after 24 h of microglia stimulation. Moreover, it induced an increase in the production of reactive oxygen species (ROS) and dysregulated antioxidant protein (i.e., SIRT-1 and NRF-2). The association between choline and adaptogens (CBW) 10 μg/mL counteracted the effect of CRH on BV2 cells and reduced the neurotoxicity produced by BV2 CRH-conditioned medium in the SH-SY5Y cell lines. CBW 200 mg/kg produced an ameliorative effect on recognition memory in the novel object recognition test (NORT) test in mice. In conclusion, combining choline with adaptogen plant extracts might represent a promising intervention in chronic stress associated with memory disturbances through the attenuation of microglia-induced oxidative stress.
Advancements in the Application of Nanomedicine in Alzheimer’s Disease: A Therapeutic Perspective
Authors: Puranik Nidhi , Yadav Dhananjay , Song Minseok
Published: September 2023

Alzheimer’s disease (AD) is a progressive neurodegenerative disease that affects most people worldwide. AD is a complex central nervous system disorder. Several drugs have been designed to cure AD, but with low success rates. Because the blood–brain and blood–cerebrospinal fluid barriers are two barriers that protect the central nervous system, their presence has severely restricted the efficacy of many treatments that have been studied for AD diagnosis and/or therapy. The use of nanoparticles for the diagnosis and treatment of AD is the focus of an established and rapidly developing field of nanomedicine. Recent developments in nanomedicine have made it possible to effectively transport drugs to the brain. However, numerous obstacles remain to the successful use of nanomedicines in clinical settings for AD treatment. Furthermore, given the rapid advancement in nanomedicine therapeutics, better outcomes for patients with AD can be anticipated. This article provides an overview of recent developments in nanomedicine using different types of nanoparticles for the management and treatment of AD.
The Change in Whole-Genome Methylation and Transcriptome Profile under Autophagy Defect and Nitrogen Starvation
Authors: Shi Yunfeng , Yu Baiyang , Cheng Shan , Hu Weiming , Liu Fen
Published: September 2023

Through whole-genome bisulfite sequencing and RNA-seq, we determined the potential impact of autophagy in regulating DNA methylation in Arabidopsis, providing a solid foundation for further understanding the molecular mechanism of autophagy and how plants cope with nitrogen deficiency. A total of 335 notable differentially expressed genes (DEGs) were discovered in wild-type Arabidopsis (Col-0-N) and an autophagic mutant cultivated under nitrogen starvation (atg5-1-N). Among these, 142 DEGs were associated with hypomethylated regions (hypo-DMRs) and were upregulated. This suggests a correlation between DNA demethylation and the ability of Arabidopsis to cope with nitrogen deficiency. Examination of the hypo-DMR-linked upregulated DEGs indicated that the expression of MYB101, an ABA pathway regulator, may be regulated by DNA demethylation and the recruitment of transcription factors (TFs; ERF57, ERF105, ERF48, and ERF111), which may contribute to the growth arrest induced by abscisic acid (ABA). Additionally, we found that DNA methylation might impact the biosynthesis of salicylic acid (SA). The promoter region of ATGH3.12 (PBS3), a key enzyme in SA synthesis, was hypomethylated, combined with overexpression of PBS3 and its potential TF AT3G46070, suggesting that autophagy defects may lead to SA-activated senescence, depending on DNA demethylation. These findings suggest that DNA hypomethylation may impact the mechanism by which Arabidopsis autophagy mutants (atg5-1) respond to nitrogen deficiency, specifically in relation to ABA and SA regulation. Our evaluation of hormone levels verified that these two hormones are significantly enriched under nitrogen deficiency in atg5-1-N compared to Col-0-N.
Regulation of Transporters for Organic Cations by High Glucose
Authors: Steinbüchel Martin , Menne Johannes , Schröter Rita , Neugebauer Ute , Schlatter Eberhard , Ciarimboli Giuliano
Published: September 2023

Endogenous positively charged organic substances, including neurotransmitters and cationic uremic toxins, as well as exogenous organic cations such as the anti-diabetic medication metformin, serve as substrates for organic cation transporters (OCTs) and multidrug and toxin extrusion proteins (MATEs). These proteins facilitate their transport across cell membranes. Vectorial transport through the OCT/MATE axis mediates the hepatic and renal excretion of organic cations, regulating their systemic and local concentrations. Organic cation transporters are part of the remote sensing and signaling system, whose activity can be regulated to cope with changes in the composition of extra- and intracellular fluids. Glucose, as a source of energy, can also function as a crucial signaling molecule, regulating gene expression in various organs and tissues. Its concentration in the blood may fluctuate in specific physiological and pathophysiological conditions. In this work, the regulation of the activity of organic cation transporters was measured by incubating human embryonic kidney cells stably expressing human OCT1 (hOCT1), hOCT2, or hMATE1 with high glucose concentrations (16.7 mM). Incubation with this high glucose concentration for 48 h significantly stimulated the activity of hOCT1, hOCT2, and hMATE1 by increasing their maximal velocity (Vmax), but without significantly changing their affinity for the substrates. These effects were independent of changes in osmolarity, as the addition of equimolar concentrations of mannitol did not alter transporter activity. The stimulation of transporter activity was associated with a significant increase in transporter mRNA expression. Inhibition of the mechanistic target of rapamycin (mTOR) kinase with Torin-1 suppressed the transporter stimulation induced by incubation with 16.7 mM glucose. Focusing on hOCT2, it was shown that incubation with 16.7 mM glucose increased hOCT2 protein expression in the plasma membrane. Interestingly, an apparent trend towards higher hOCT2 mRNA expression was observed in kidneys from diabetic patients, a pathology characterized by high serum glucose levels. Due to the small number of samples from diabetic patients (three), this observation must be interpreted with caution. In conclusion, incubation for 48 h with a high glucose concentration of 16.7 mM stimulated the activity and expression of organic cation transporters compared to those measured in the presence of 5.6 mM glucose. This stimulation by a diabetic environment could increase cellular uptake of the anti-diabetic drug metformin and increase renal tubular secretion of organic cations in an early stage of diabetes.
Early Pregnancy Markers in the Serum of Ewes Identified via Proteomic and Metabolomic Analyses
Authors: Zhai Yaying , Xia Fan , Shi Luting , Ma Wenkui , Lv Xiaoyang , Sun Wei , Ji Pengyun , Gao Shuai , Machaty Zoltan , Liu Guoshi , Zhang Lu
Published: September 2023

The diagnosis of ewes’ pregnancy status at an early stage is an efficient way to enhance the reproductive output of sheep and allow producers to optimize production and management. The techniques of proteomics and metabolomics have been widely used to detect regulatory factors in various physiological processes of animals. The aim of this study is to explore the differential metabolites and proteins in the serum of pregnant and non-pregnant ewes by proteomics and metabolomics. The serum of ewes at 21, 28 and 33 days after artificial insemination (AI) were collected. The pregnancy stratus of the ewes was finally determined through ultrasound examination and then the ewes were grouped as Pregnant (n = 21) or N on-pregnant (n = 9). First, the serum samples from pregnant or non-pregnant ewes at 21 days after AI were selected for metabolomic analysis. It was found that the level of nine metabolites were upregulated and 20 metabolites were downregulated in the pregnant animals (p < 0.05). None of these differential metabolomes are suitable as markers of pregnancy due to their small foldchange. Next, the proteomes of serum from pregnant or non-pregnant ewes were evaluated. At 21 days after AI, the presence of 321 proteins were detected, and we found that the level of three proteins were upregulated and 11 proteins were downregulated in the serum of pregnant ewes (p < 0.05). The levels of serum amyloid A (SAA), afamin (AFM), serpin family A member 6 (SERPINA6) and immunoglobulin-like domain-containing protein between pregnant and non-pregnant ewes at 21-, 28- and 33-days post-AI were also analyzed via enzyme-linked immunosorbent assay (ELISA). The levels of SAA and AFM were significantly higher in pregnant ewes than in non-pregnant ewes, and could be used as markers for early pregnancy detection. Overall, our results show that SAA and AFM are potential biomarkers to determine the early pregnancy status of ewes.
A Robust Drug–Target Interaction Prediction Framework with Capsule Network and Transfer Learning
Authors: Huang Yixian , Huang Hsi-Yuan , Chen Yigang , Lin Yang-Chi-Dung , Yao Lantian , Lin Tianxiu , Leng Junlin , Chang Yuan , Zhang Yuntian , Zhu Zihao , Ma Kun , Cheng Yeong-Nan , Lee Tzong-Yi , Huang Hsien-Da
Published: September 2023

Drug–target interactions (DTIs) are considered a crucial component of drug design and drug discovery. To date, many computational methods were developed for drug–target interactions, but they are insufficiently informative for accurately predicting DTIs due to the lack of experimentally verified negative datasets, inaccurate molecular feature representation, and ineffective DTI classifiers. Therefore, we address the limitations of randomly selecting negative DTI data from unknown drug–target pairs by establishing two experimentally validated datasets and propose a capsule network-based framework called CapBM-DTI to capture hierarchical relationships of drugs and targets, which adopts pre-trained bidirectional encoder representations from transformers (BERT) for contextual sequence feature extraction from target proteins through transfer learning and the message-passing neural network (MPNN) for the 2-D graph feature extraction of compounds to accurately and robustly identify drug–target interactions. We compared the performance of CapBM-DTI with state-of-the-art methods using four experimentally validated DTI datasets of different sizes, including human (Homo sapiens) and worm (Caenorhabditis elegans) species datasets, as well as three subsets (new compounds, new proteins, and new pairs). Our results demonstrate that the proposed model achieved robust performance and powerful generalization ability in all experiments. The case study on treating COVID-19 demonstrates the applicability of the model in virtual screening.