bluuebunny/crossref_metadata_2025 huggingface dataset:
Qualitative systematic review of general practitioners’ (GPs’) views and experiences of providing postnatal care
Authors: Macdonald Clare , MacGregor Becky , Hillman Sarah , MacArthur Christine , Bick Debra , Taylor Beck
Published: April 2023

Objectives

Develop an understanding of the views and experiences of general practitioners (GPs) about their role in postnatal care, including barriers and facilitators to good care, and timing and content of planned postnatal checks.

Design

Qualitative systematic review.

Data sources

Electronic database searches of MEDLINE, EMBASE, CINAHL, PubMed, Web of Science, PsychINFO from January 1990 to September 2021. Grey literature and guideline references from National Institute of Health and Care Excellence, WHO, International Federation of Gynecology and Obstetrics, Royal College of General Practitioners, Royal College of Obstetrics and Gynaecology.

Inclusion criteria

Papers reporting qualitative data on views and experiences of GPs about postnatal care, including discrete clinical conditions in the postnatal period. Papers were screened independently by two reviewers and disputes resolved by a third reviewer.

Quality appraisal

The Critical Appraisal Skills Programme checklist was used to appraise studies.

Data extraction and synthesis

Thematic synthesis involving line-by-line coding, generation of descriptive then analytical themes was conducted by the review team. The Capability, Opportunity, Motivation-Behaviour (COM-B) model was used to develop analytical themes.

Results

20 reports from 18 studies met inclusion criteria. Studies were published from 2008 to 2021, reporting on 469 GPs. 13 were from UK or Australia. Some also reported views of non-GP participants. The clinical focus of studies varied, for example: perinatal mental health, postnatal contraception. Five themes were generated, four mapped to COM-B: psychological capability, physical opportunity, social opportunity and motivation. One theme was separate from the COM-B model: content and timing of postnatal checks. Strong influences were in physical and social opportunity, with time and organisation of services being heavily represented. These factors sometimes influenced findings in the motivation theme.

Conclusions

GPs perceived their role in postnatal care as a positive opportunity for relationship building and health promotion. Addressing organisational barriers could impact positively on GPs’ motivation to provide the best care.

PROSPERO registration number

268982.
Methods for evaluation of corneal nerve fibres in diabetes mellitus by in vivo confocal microscopy: a scoping review protocol
Authors: Sandvik Siv Aaseth , Sundling Vibeke , Lagali Neil
Published: April 2023

Introduction

Globally, 422 million people have diabetes. Late complications of diabetes are blindness, kidney failure, heart attack, stroke and lower limb amputation. The prevalence of diabetic peripheral neuropathy and diabetic retinopathy is 50% and 35%, respectively. In vivo confocal microscopy (IVCM) is a rapid, non-invasive method to evaluate subbasal corneal nerve fibres, which are small fibres of the peripheral nervous system. Corneal nerve fibre changes can be a marker of diabetic peripheral neuropathy. There is currently no gold-standard procedure for IVCM imaging, image processing or quantitative analysis of the corneal nerve fibres in the subbasal plexus. This protocol describes a scoping review to map, summarise and critically evaluate current methods used with IVCM evaluation in people with diabetes mellitus.

Methods

The scoping review will follow Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines for scoping review. A comprehensive search of the literature will be conducted in MEDLINE, Embase, Cochrane, Scopus and Web of Science. The search strategy will include terms related to IVCM, diabetes and corneal nerve fibres. We will set inclusion and exclusion criteria prior to the search, and two reviewers will screen titles and abstracts independently. One reviewer will full text read eligible articles and chart data from the studies. A descriptive summary of the methods used in imaging, image processing and quantitative analysis of peripheral corneal nerve fibres by IVCM will be written.

Ethics and dissemination

Ethical approval is not required since this is a scoping review based on previously published articles. The findings will be published in a scientific peer-reviewed journal.
Cohort profile: SUPER-Finland – the Finnish study for hereditary mechanisms of psychotic disorders
Authors: Lähteenvuo Markku , Ahola-Olli Ari , Suokas Kimmo , Holm Minna , Misiewicz Zuzanna , Jukuri Tuomas , Männynsalo Teemu , Wegelius Asko , Haaki Willehard , Kajanne Risto , Kyttälä Aija , Tuulio-Henriksson Annamari , Lahdensuo Kaisla , Häkkinen Katja , Hietala Jarmo , Paunio Tiina , Niemi-Pynttäri Jussi , Kieseppä Tuula , Veijola Juha , Lönnqvist Jouko , Isometsä Erkki , Kampman Olli , Tiihonen Jari , Hyman Steven , Neale Benjamin , Daly Mark , Suvisaari Jaana , Palotie Aarno
Published: April 2023

Purpose

SUPER-Finland is a large Finnish collection of psychosis cases. This cohort also represents the Finnish contribution to the Stanley Global Neuropsychiatric Genetics Initiative, which seeks to diversify genetic sample collection to include Asian, Latin American and African populations in addition to known population isolates, such as Finland.

Participants

10 474 individuals aged 18 years or older were recruited throughout the country. The subjects have been genotyped with a genome-wide genotyping chip and exome sequenced. A subset of 897 individuals selected from known population sub-isolates were selected for whole-genome sequencing. Recruitment was done between November 2015 and December 2018.

Findings to date

5757 (55.2%) had a diagnosis of schizophrenia, 944 (9.1%) schizoaffective disorder, 1612 (15.5%) type I or type II bipolar disorder, 532 (5.1 %) psychotic depression, 1047 (10.0%) other psychosis and for 530 (5.1%) self-reported psychosis at recruitment could not be confirmed from register data. Mean duration of schizophrenia was 22.0 years at the time of the recruitment. By the end of the year 2018, 204 of the recruited individuals had died. The most common cause of death was cardiovascular disease (n=61) followed by neoplasms (n=40). Ten subjects had psychiatric morbidity as the primary cause of death.

Future plans

Compare the effects of common variants, rare variants and copy number variations (CNVs) on severity of psychotic illness. In addition, we aim to track longitudinal course of illness based on nation-wide register data to estimate how phenotypic and genetic differences alter it.
Understanding the traditional values and use of okra among pregnant women in western Ethiopia: a qualitative study
Authors: Negash Kushi Efrem , Belachew Tefera , Tamiru Dessalegn
Published: April 2023

Objectives

This study explored the traditional values and use of okra among pregnant women, how okra plants are obtained, prepared and used by pregnant women, and the associated beliefs and meanings attached to it in western Ethiopia.

Design

Qualitative research.

Setting

Rural areas of western Ethiopia.

Participants

A purposive sampling technique was used to select a total of 86 pregnant women (14 for in-depth interviews and 72 for focus group discussions) in western Ethiopia.

Results

Traditionally okra is used as a source of income and is a common food for guests visiting homes. In line with this, pregnant women in the western part of Ethiopia mainly consumed okra pods. For future consumption and preservation for a long period, they usually transform okra into powder.

Conclusions

Other parts of the okra plant rather than pods are not known as a food source and are the most neglected food sources in rural districts of western Ethiopia. The study provides evidence that supports nutritional behavioural change communication interventions on promoting the utilisation of different parts of okra and awareness creation on the nutritional values of okra.
Specific recognition of anFGFR2fusion by tumor infiltrating lymphocytes from a patient with metastatic cholangiocarcinoma
Authors: White Bradley Sinclair , Sindiri Sivasish , Hill Victoria , Gasmi Billel , Nah Shirley , Gartner Jared J , Prickett Todd D , Li Yong , Gurusamy Devikala , Robbins Paul , Rosenberg Steven A , Leko Vid
Published: April 2023

Background

Metastatic cholangiocarcinoma (CC), a form of gastrointestinal cancer that originates from the bile ducts, cannot be cured by currently available therapies, and is associated with dismal prognosis. In a previous case report, adoptive transfer of autologous tumor infiltrating lymphocytes (TILs), the majority of which recognized a tumor-specific point mutation, led to a profound and durable cancer regression in a patient with metastatic CC. Thus, more effective treatment for patients with this disease may be developed by using TILs that target cancer-specific mutations, but also other genetic aberrations such as gene fusions. In this context, fusions that involvefibroblast growth factor receptor 2(FGFR2) and function as oncogenes in a subset of patients with intrahepatic CC (ICC) represent particularly attractive targets for adoptive cell therapy. However, no study to date has explored whetherFGFR2fusions can be recognized by patients’ T cells.

Method

To address whetherFGFR2fusions can be recognized by patients’ T cells, we tested TILs from four patients withFGFR2fusion-positive ICC for recognition of peptides and minigenes that represented the breakpoint regions of these fusions, which were unique to each of the four patients.

Results

We found that CD4+TILs from one patient specifically recognized the breakpoint region of a uniqueFGFR2-TDRD1 (tudor domain-containing 1)fusion, and we isolated a T-cell receptor responsible for its recognition.

Conclusions

This finding suggests thatFGFR2fusion-reactive TILs can be isolated from some patients with metastatic ICC, and thus provides a rationale for future exploration of T cell-based therapy targetingFGFR2fusions in patients with cancer. Furthermore, it augments the rationale for extending such efforts to other types of solid tumors hallmarked by oncogenic gene fusions.
Density functional theory study of the VmN2On (m,n = 1,2) complexes in silicon
Authors: Christopoulos S.-R. G. , Sgourou E. N. , Chroneos A. , Londos C. A.
Published: April 2023

Nitrogen is an important impurity in Czochralski grown silicon (Cz–Si) as it enhances oxygen precipitation through the formation of vacancy–nitrogen–oxygen clusters and in particular the [Formula: see text] complexes. Here, we employ density functional theory (DFT) to predict the structure of [Formula: see text] ([Formula: see text]). We report that the lowest energy [Formula: see text] ([Formula: see text]) defects are very strongly bound. These results are consistent, and support the previously reported theoretical and experimental conclusions that [Formula: see text] structures could form.
MUST HAVE OR NICE TO HAVE? NECESSARY LEADERSHIP COMPETENCIES TO ENABLE EMPLOYEES’ INNOVATIVE BEHAVIOUR
Authors: LIEHR JENNIFER , HAUFF SVEN
Published: April 2023

Encouraging employees’ innovative behaviour (EIB) is one of the most important leadership tasks and numerous studies have examined the relationships between leadership and EIB. However, the research field is highly fragmented and does not provide a clear answer as to which leadership competencies are actually crucial for EIB. To address this issue, we use a qualitative content analysis to examine which leadership competencies are characterised in the literature as necessary for EIB. In doing so, we also turn away from broad leadership styles and adopt a behavioural-focussed competency approach. The results show that only a very small number of the many leadership competencies that seem to support EIB are considered necessary prerequisites for EIB. Based on the results, we present seven theoretical propositions and a concise model of leadership competencies necessary for EIB. In particular, we propose that the leadership competencies of providing support, communicating a vision, granting autonomy and discretion, and providing feedback are necessary conditions to enable EIB.
THE IMPACTS OF BOARD FAULTLINES ON INNOVATION PERFORMANCE IN CROSS-BORDER MERGERS AND ACQUISITIONS
Authors: XIAO HUIMIN , SHI YUETING , YANG TINGQING
Published: April 2023

Based on the team faultlines theory, this paper examines the impact of different types of board faultlines on innovation in cross-border mergers and acquisitions (M&As). Using the data of Chinese firms’ cross-border M&As, this paper finds that information-based faultlines and social category faultlines have an inverted U-shaped relationship with innovation in cross-border M&As. Cultural distance weakens the role of information-based faultlines on innovation in cross-border M&As. This paper enriches the research on the factors influencing innovation in cross-border M&As and provides a basis for firms to select board members and create suitable board faultlines.
Current applications using key mineral phases in igneous and metamorphic geology: perspectives for the future
Authors: Volante Silvia , Blereau Eleanore , Guitreau Martin , Tedeschi Mahyra , van Schijndel Valby , Cutts Kathryn
Published: April 2023

The study of magmatic and metamorphic processes is challenged by geological complexities like geochemical variations, geochronological uncertainties and the presence/absence of fluids and/or melts. However, by integrating petrographic and microstructural studies with geochronology, geochemistry and phase equilibrium diagram investigations of different key mineral phases, it is possible to reconstruct insightful pressure–temperature–deformation–time histories. Using multiple geochronometers in a rock can provide a detailed temporal account of its evolution, as these geological clocks have different closure temperatures. Given the continuous improvement of existing and new
in situ
analytical techniques, this contribution provides an overview of frequently utilized petrochronometers such as garnet, zircon, titanite, allanite, rutile, monazite/xenotime and apatite, by describing the geological record that each mineral can retain and explaining how to retrieve this information. These key minerals were chosen as they provide reliable age information in a variety of rock types and, when coupled with their trace element (TE) composition, form powerful tools to investigate crustal processes at different scales. This review recommends best applications for each petrochronometer, highlights limitations to be aware of and discusses future perspectives. Finally, this contribution underscores the importance of integrating information retrieved by multi-petrochronometer studies to gain an in-depth understanding of complex thermal and deformation crustal processes.
Using Generative AI to Cultivate Positive Emotions and Mindsets for Self-Development and Learning
Authors: Leong Joanne
Published: April 2023

The arrival of new generative AI tools is creating waves. Here are some ideas for how we could channel them for supporting self-development and learning.
Collaborative Fuzzy Linguistic Learning to Low-Resource and Robust Decision System Based on Bounded Rationality
Authors: Zhang Chao , Li Xiaochuan , Sangaiah Arun Kumar , Li Wentao , Wang Baoli , Cao Feng , Shangguan Xuekui
Published: April 2023

Low-resource languages are challenging to process intelligent decision systems due to limited data and resources. As an effective way of processing low-resource languages in intelligent decision systems, fuzzy linguistic approaches excel in transforming original uncertain linguistic information into highly structured data and learning valid decision rules between complex data structures. However, existing fuzzy linguistic methods may not fully capture realistic features of multi-attribute group decision-making (MAGDM), such as incomplete and hesitant linguistic expressions, stable information fusion, and bounded rationality of decision-makers (DMs). Therefore, it is necessary to develop a collaborative fuzzy language learning system based on bounded rationality, low-resource and robust decision-making. Specifically, we present a new multi-granularity (MG) group decision-making (GDM) scheme by using MULTIMOORA (Multi-Objective Optimization by Ratio Analysis plus the full MULTIplicative form) and PT (Prospect Theory) for incomplete hesitant fuzzy linguistic information systems (I-HFL-ISs), where MG GDM aims to discover knowledge from complex MAGDM problems with MG features. To achieve the above goal, we first introduce the concept of MG-I-HFL-ISs to represent incomplete, hesitant and imprecise linguistic evaluation information offered by multiple decision-makers (DMs). Then, we apply a valid transformation scheme to convert MG-I-HFL-ISs into MG-HFL-ISs, and use the MG probability rough set (PRS) to develop a series of MG-HFL-PRSs with the support of MULTIMOORA. Afterwards, an HFL MG GDM method is designed by integrating MULTIMOORA and PT for solving MAGDM problems with MG-I-HFL-ISs. The proposed method can effectively synthesize low-resource languages and mine useful decision-making knowledge. At last, a drug selection case and a simulated case are performed for showing the rationality of the designed HFL MG GDM scheme.
Effectiveness of Antiretroviral Treatment on the Transition Probability of Immunological State of HIV/AIDS Patients: A Markov Chain Model on the Iranian “National Registry of HIV/AIDS Care” Database
Authors: Mirahmadizadeh Alireza , Sharafi Mehdi , Hassanzadeh Jafar , Seif Mozhgan , Heiran Alireza
Published: April 2023

Antiretroviral therapy (ART) has improved survival and clinical course amongst HIV/AIDS patients. CD4 cell count is one of the most critical indicators of the disease progression. With respect to the dynamic nature of CD4 cell count during the clinical history of HIV/AIDS, modeling the CD4 cell count changes, which represents the likelihood of disease progression, is of interest to establish or revise treatment strategies and, specifically, to determine the stage at which giving ART is more clinically effective. In this historical cohort study on 917 HIV/AIDS patients in the Iranian “National Registry of HIV/AIDS Care” database, we used the Markov chain model to predict the effectiveness of the ART based on the transition probability of CD4 cell count, measured before and after initiating ART. We found that when the ART was initiated in the earlier stages of HIV infection, good prognosis might be more accessible; that is, after initiating ART at state CD4 ≥ 500, the probability of staying at this state was statistically increased than before the treatment (P < 0.001). Also, it was found that initiating ART significantly decreased the probability of CD4 cell count transition to the lower counts (PCD4≥500‐to‐350≤CD4<500 < 0.001, P350≤CD4<500‐to‐200≤CD4<350 < 0.001, and P200≤CD4<350‐to‐CD4<200 < 0.001). In addition, initiating ART showed a statistically significant increase in the probability of CD4 cell count transition from a lower state (350 ≤ CD4 < 500) to a higher state (CD4 ≥ 500) (P = 0.009). Furthermore, When CD4 count reaches under 200, even after the initiation of therapy, the probability of CD4 cell count transition to higher levels was not significant (P > 0.05). In summary, these results have a message for primary healthcare organizations to extensively identify HIV patients and initiating ART as early as possible.
The Residual Effect of C60 Fullerene on Biomechanical and Biochemical Markers of the Muscle Soleus Fatigue Development in Rats
Authors: Prylutskyy Yuriy , Nozdrenko Dmytro , Gonchar Olga , Prylutska Svitlana , Bogutska Kateryna , Täuscher Eric , Scharff Peter , Ritter Uwe
Published: April 2023

Muscle fatigue as a defense body mechanism against overload is a result of the products of incomplete oxygen oxidation such as reactive oxygen species. Hence, C60 fullerene as a powerful nanoantioxidant can be used to speed up the muscle recovery process after fatigue. Here, the residual effect of C60 fullerene on the biomechanical and biochemical markers of the development of muscle soleus fatigue in rats for 2 days after 5 days of its application was studied. The known antioxidant N-acetylcysteine (NAC) was used as a comparison drug. The atomic force microscopy to determine the size distribution of C60 fullerenes in an aqueous solution, the tensiometry of skeletal muscles, and the biochemical analysis of their tissues and rat blood were used in this study. It was found that after the cessation of NAC injections, the value of the integrated muscle power is already slightly different from the control (5%–7%) on the first day, and on the second day, it does not significantly differ from the control. At the same time, after the cessation of C60 fullerene injections, its residual effect was 45%–50% on the first day, and 17%–23% of the control on the second one. A significant difference (more than 25%) between the pro- and antioxidant balance in the studied muscles and blood of rats after the application of C60 fullerene and NAС plays a key role in the long-term residual effect of C60 fullerene. This indicates prolonged kinetics of C60 fullerenes elimination from the body, which contributes to their long-term (at least 2 days) compensatory activation of the endogenous antioxidant system in response to muscle stimulation, which should be considered when developing new therapeutic agents based on these nanoparticles.
The Consecutive Substitution Method for Boundary Value Problems (BVPs) with Retarded Argument
Authors: Aykut Arzu , Çelik Ercan
Published: April 2023

In this study, we applied an approximate solution method for solving the boundary value problems (BVPs) with retarded argument. The method is the consecutive substitution method. The consecutive substitution method was applied and an approximate solution was obtained. The numerical solution and the analytical solution are compared in the table. The solutions were found to be compatible.
Triple Band-Notch UWB Antenna Embedded with Slot and EBG Structures
Authors: Babu P. Raveendra , Ramakrishna D. , Ensermu Ginbar
Published: April 2023

In this paper, a planar, compact pentagonal shaped ultrawideband antenna of microstrip line fed offering triple band-notched characteristics response is proposed and investigated. Triple band-notch response can be achieved by creating two inverted U-shaped slots of different size in pentagonal patch, and also electromagnetic band gap structure of hexagonal shape is created near the feed line of UWB antenna. To implement the proposed antenna, RT/DUROID 5880 substrate of 1.6 mm thickness is used. The designed antenna was successfully simulated, developed, and manufactured. The dimension of the suggested antenna is

36
 
mm
×
33
 
mm
×
1.6
 
mm

and has a bandwidth of 3.1–10.6 GHz with a magnitude of

S
11
<
−
10
 
dB

, the maximum pass band gain of 4.6 dB and with the exception of the 4.0–4.4 GHz (C-band satellite communication), 5.2–5.8 GHz (WLAN), and 8.0–8.25 GHz (X-band) frequency bands. The suggested antenna has a good return loss, a virtually omnidirectional radiation pattern, and a constant gain throughout operation.
Association between Circulating Ectodysplasin A and Diabetic Kidney Disease
Authors: Deng Xia , Guo Chang , Qin Huijuan , Zhao Li , Li Yanyan , Zhao Zhicong , Li Haoxiang , Yang Ling , Wang Dong , Yuan Guoyue
Published: April 2023

Background. Ectodysplasin A (EDA), a member of the TNF family, plays important roles in ectodermal development, while recent studies expanded its regulatory effects on insulin resistance and lipid metabolism. This study was the first time to investigate the correlation between circulating EDA and albuminuria in patients with T2DM. Methods. A total of 189 T2DM and 59 healthy subjects were enrolled in the study. We analyzed the concentrations of EDA by ELISA. Plasma glucose, insulin, HbA1c, lipids, creatinine, BUN, and UACR were also measured. Insulin resistance and pancreatic cell function were assessed by HOMA. Results. Circulating EDA concentration was significantly increased in T2DM patients and increased with the degree of albuminuria. EDA was positively correlated with age, FIns, HOMA-IR, HOMA-β, Scr, and UACR, and negatively correlated with eGFR. Linear stepwise regression showed that FIns, HOMA-β, and UACR were independent influencing factors of EDA. Logistic regression analysis showed that EDA was independently associated with the occurrence of albuminuria in T2DM. ROC curve showed that EDA had an area under the receiver operating curve of 0.701 [

95
%
CI
=

0.625
−
0.777

,

P
<
0.001

]. Conclusion. EDA is positively correlated with the degree of albuminuria in patients with T2DM and may be involved in the occurrence and progression of diabetic kidney disease (DKD).
Theoretical and Experimental Analysis of a Winding Rope Fluid Viscous Damper
Authors: Zhang Cheng , Zhang Wenxue , Su Lijun , Chen Ying , Du Xiuli
Published: April 2023

This paper investigated a winding rope fluid viscous damper (WRFVD) that uses the frictional amplification mechanism of a winding rope to obtain the operating characteristics of a viscous damper-like device. The WRFVD combined the advantages of fluid viscous dampers and friction dampers. It not only retained the mechanical characteristics of the fluid viscous damper but also reduced manufacturing costs. First, the construction and working principles of the WRFVD were introduced. A theoretical model that could accurately simulate the hysteretic characteristics of the damper was derived. Then, a series of dynamic tests were performed on six prototypes of the WRFVD. The dynamic performance under different displacement amplitudes and device parameters was analyzed based on the test results. In addition, the theoretical model was validated by experimental results. Finally, a series of parametric analyses of the WRFVD were performed. The experimental results showed that the WRFVD is a type of velocity-dependent damper with smooth and plump hysteretic curves under sinusoidal displacement excitation. Fatigue loading tests showed that the WRFVD had excellent fatigue resistance capacity and stable operational performance. The established theoretical model was reasonable and satisfactorily reproduced the hysteretic properties of the WRFVD. The parametric analyses showed that it was not recommended to improve the performance of the WRFVD by the method of adjusting the damping coefficient and pretightening load. It was reasonable to adjust the performance of the WRFVD by setting a smaller velocity exponent, an appropriate winding turn of winding ropes, and a suitable friction coefficient.
A Real-Time AIS Data Cleaning and Indicator Analysis Algorithm Based on Stream Computing
Authors: Lv Taizhi , Tang Peiyi , Zhang Juan
Published: April 2023

The data quality and real-time analysis of automatic identification system (AIS) are of great significance for water transportation safety and intelligent maritime construction. To improve the AIS data quality and analyze AIS data in real time, a real-time AIS data cleaning and indicator analysis algorithm is proposed. This algorithm performs distributed real-time data cleaning and analysis for massive AIS data based on stream computing technology. It includes data fusion, deduplication, decoding, abnormal data identification, sequencing, prediction, and statistics steps. Abnormal AIS data are repaired by linear regression, multiple trajectory tracking, caching, and other technologies. The AIS status is determined in real-time via multidimensional AIS packet loss analyses, multifactor AIS data statistics, and spatial-temporal data visualization, effectively improving the intelligence level of maritime supervision applications. The proposed algorithm has been running on a production environment, and it monitors AIS data in a certain section of the Yangtze River Basin 24 hours every day without interruption. The operation results show that the proposed algorithm can improve the quality of AIS data, addresses ship trajectory jump issues, and provides timely position updates. The real-time indicator analysis results can provide the data support for ship navigation and maritime supervision.
Human Infrapatellar Fat Pad Mesenchymal Stem Cell-Derived Extracellular Vesicles Inhibit Fibroblast Proliferation by Regulating MT2A to Reduce Knee Arthrofibrosis
Authors: Jia Dazhou , Chen Hui , Dai Jihang , He Shiping , Liu Yangyang , Liu Zhendong , Zhang Yaxin , Li Xiaolei , Sun Yu , Wang Qiang
Published: April 2023

Knee arthrofibrosis is one of the most serious complications of knee surgery; however, its pathogenesis is unclear, and current treatment methods have not achieved satisfactory results. Mesenchymal stem cells (MSCs) have good anti-inflammatory and antifibrotic properties, and studies have reported that human infrapatellar fat pad-derived MSCs (IPFSCs) have the advantages of strong proliferative and differentiating ability, ease of acquisition, and minimal harm to the donor. Increasing evidence has shown that MSCs function through their paracrine extracellular vesicles (EVs). Our study is aimed at exploring the effects of human IPFSC-derived EVs (IPFSC-EVs) on knee arthrofibrosis and the underlying mechanisms in vivo and in vitro. In the in vivo study, injecting IPFSC-EVs into the knee joint cavity effectively reduced surgery-induced knee arthrofibrosis in rats. In the in vitro study, IPFSC-EVs were found to inhibit the proliferation of fibroblasts in the inflammatory environment. Additionally, we screened a potential IPFSC-EV molecular target, metallothionein 2A (MT2A), using RNA sequencing. We found that silencing MT2A partially reversed the inhibitory effect of IPFSC-EVs on fibroblast proliferation in the inflammatory environment. In conclusion, IPFSC-EVs inhibit the progression of knee arthrofibrosis by regulating MT2A, which inhibits fibroblast proliferation in the inflammatory environment.
Table S5 from Trend in Cervical Cancer Incidence and Mortality Rates in China, 2006–2030: A Bayesian Age-Period-Cohort Modeling Study
Authors: Yuan Meiwen , Zhao Xuelian , Wang Honghao , Hu Shangying , Zhao Fanghui
Published: April 2023

Table S5 shows proportion of the annual number of deaths by age group between 2006 to 2016.
Table S4 from Trend in Cervical Cancer Incidence and Mortality Rates in China, 2006–2030: A Bayesian Age-Period-Cohort Modeling Study
Authors: Yuan Meiwen , Zhao Xuelian , Wang Honghao , Hu Shangying , Zhao Fanghui
Published: April 2023

Table S4 shows average age and standardized average age at death for cervical cancer between 2006 to 2030.
Supplementary Table 10 from Associations between MICA and MICB Genetic Variants, Protein Levels, and Colorectal Cancer: Atherosclerosis Risk in Communities (ARIC)
Authors: Wang Shuo , Onyeaghala Guillaume C. , Pankratz Nathan , Nelson Heather H. , Thyagarajan Bharat , Tang Weihong , Norby Faye L. , Ugoji Chinenye , Joshu Corinne E. , Gomez Christian R. , Couper David J. , Coresh Josef , Platz Elizabeth A. , Prizment Anna E.
Published: April 2023

Supplemental Table 10 shows that associations between mean of Visit 2 and Visit 3 sMICA and sMICB levels (continuous variables) with CRC risk.
Supplementary Table 1 from Associations between MICA and MICB Genetic Variants, Protein Levels, and Colorectal Cancer: Atherosclerosis Risk in Communities (ARIC)
Authors: Wang Shuo , Onyeaghala Guillaume C. , Pankratz Nathan , Nelson Heather H. , Thyagarajan Bharat , Tang Weihong , Norby Faye L. , Ugoji Chinenye , Joshu Corinne E. , Gomez Christian R. , Couper David J. , Coresh Josef , Platz Elizabeth A. , Prizment Anna E.
Published: April 2023

Supplemental Table 1 shows the results from previous studies that found significant associations between the pre-selected SNPs and risk of cancers or autoimmune diseases.
Figure S1 from Trend in Cervical Cancer Incidence and Mortality Rates in China, 2006–2030: A Bayesian Age-Period-Cohort Modeling Study
Authors: Yuan Meiwen , Zhao Xuelian , Wang Honghao , Hu Shangying , Zhao Fanghui
Published: April 2023

Figure S1 shows trends for incidence cases during 2006−2016 and predicted from 2017 to 2030 in China.
Supplemental Table 1 from Measurement of Ovarian Tumor Immune Profiles by Multiplex Immunohistochemistry: Implications for Epidemiologic Studies
Authors: Hathaway Cassandra A. , Conejo-Garcia Jose R. , Fridley Brooke L. , Rosner Bernard , Saeed-Vafa Daryoush , Moran Segura Carlos , Nguyen Jonathan V. , Hecht Jonathan L. , Sasamoto Naoko , Terry Kathryn L. , Tworoger Shelley S. , Townsend Mary K.
Published: April 2023

Supplemental Table 1 shows the correlations of percentages of the markers in the tumor.
Data from Measurement of Ovarian Tumor Immune Profiles by Multiplex Immunohistochemistry: Implications for Epidemiologic Studies
Authors: Hathaway Cassandra A. , Conejo-Garcia Jose R. , Fridley Brooke L. , Rosner Bernard , Saeed-Vafa Daryoush , Moran Segura Carlos , Nguyen Jonathan V. , Hecht Jonathan L. , Sasamoto Naoko , Terry Kathryn L. , Tworoger Shelley S. , Townsend Mary K.
Published: April 2023

AbstractBackground:
Despite the immunogenic nature of many ovarian tumors, treatment with immune checkpoint therapies has not led to substantial improvements in ovarian cancer survival. To advance population-level research on the ovarian tumor immune microenvironment, it is critical to understand methodologic issues related to measurement of immune cells on tissue microarrays (TMA) using multiplex immunofluorescence (mIF) assays.
Methods:
In two prospective cohorts, we collected formalin-fixed, paraffin-embedded ovarian tumors from 486 cases and created seven TMAs. We measured T cells, including several sub-populations, and immune checkpoint markers on the TMAs using two mIF panels. We used Spearman correlations, Fisher exact tests, and multivariable-adjusted beta-binomial models to evaluate factors related to immune cell measurements in TMA tumor cores.
Results:
Between-core correlations of intratumoral immune markers ranged from 0.52 to 0.72, with more common markers (e.g., CD3+, CD3+CD8+) having higher correlations. Correlations of immune cell markers between the whole core, tumor area, and stromal area were high (range 0.69–0.97). In multivariable-adjusted models, odds of T-cell positivity were lower in clear cell and mucinous versus type II tumors (ORs, 0.13–0.48) and, for several sub-populations, were lower in older tissue (sample age > 30 versus ≤ 10 years; OR, 0.11–0.32).
Conclusions:
Overall, high correlations between cores for immune markers measured via mIF support the use of TMAs in studying ovarian tumor immune infiltration, although very old samples may have reduced antigenicity.
Impact:
Future epidemiologic studies should evaluate differences in the tumor immune response by histotype and identify modifiable factors that may alter the tumor immune microenvironment.
Table S4 from PSMA-positive Circulating Tumor Cell Detection and Outcomes with Abiraterone or Enzalutamide Treatment in Men with Metastatic Castrate-resistant Prostate Cancer
Authors: Gupta Santosh , Halabi Susan , Yang Qian , Roy Akash , Tubbs Alisa , Gore Yamini , George Daniel J. , Nanus David M. , Antonarakis Emmanuel S. , Danila Daniel C. , Szmulewitz Russell Z. , Wenstrup Richard , Armstrong Andrew J.
Published: April 2023

CTC biomarker group incidence by PSMA optimal cutoff (AR-v7, chromosomal instability (CIN), and neuroendocrine (NE) at baseline (N=97) and progression (N=57).
Table S2 from PSMA-positive Circulating Tumor Cell Detection and Outcomes with Abiraterone or Enzalutamide Treatment in Men with Metastatic Castrate-resistant Prostate Cancer
Authors: Gupta Santosh , Halabi Susan , Yang Qian , Roy Akash , Tubbs Alisa , Gore Yamini , George Daniel J. , Nanus David M. , Antonarakis Emmanuel S. , Danila Daniel C. , Szmulewitz Russell Z. , Wenstrup Richard , Armstrong Andrew J.
Published: April 2023

The prevalence of PSMA and Cell Search CTC positivity (≥5) at baseline and progression, as well as the observed median and optimal cutoffs for PSMA CTC.
Data from PSMA-positive Circulating Tumor Cell Detection and Outcomes with Abiraterone or Enzalutamide Treatment in Men with Metastatic Castrate-resistant Prostate Cancer
Authors: Gupta Santosh , Halabi Susan , Yang Qian , Roy Akash , Tubbs Alisa , Gore Yamini , George Daniel J. , Nanus David M. , Antonarakis Emmanuel S. , Danila Daniel C. , Szmulewitz Russell Z. , Wenstrup Richard , Armstrong Andrew J.
Published: April 2023

AbstractPurpose:
In men with metastatic castration-resistant prostate cancer (mCRPC), prostate-specific membrane antigen (PSMA)-targeted radioligand therapy has drastically improved clinical outcomes. A liquid biopsy characterizing PSMA expression could be useful in guiding optimal therapy.
Experimental Design:
We conducted a retrospective analysis of the prospective multicenter PROPHECY (Prospective CiRculating PrOstate Cancer Predictors in HighEr Risk mCRPC StudY) trial of men with mCRPC (n = 118) treated with abiraterone (abi) or enzalutamide (enza). Circulating tumor cells (CTC) were enriched (CTC/mL) and characterized for PSMA protein expression/heterogeneity at baseline and progression. We utilized proportional hazards modeling of the association between PSMA-positive (PSMA+) CTC enumeration with overall survival (OS) and progression-free survival (PFS).
Results:
Overall, 97 men with mCRPC had evaluable blood samples for baseline CTC PSMA detection; 78 men (80%) had detectable CTCs. Of these, 55% (43/78) of men had any PSMA CTC detection, 21% (16/78) had ≥2 PSMA+ CTCs/mL, and 19% (8/43) were 100% PSMA+. At progression on abi/enza, 88% (50/57) of men had detectable CTCs, 68% (34/50) had any PSMA CTCs, and 12% (4/34) had 100% PSMA+ CTCs. Among paired cases (n = 57), PSMA+ CTC detection increased slightly after abi/enza progression. Using an optimal cutoff of ≥2 PSMA+ CTCs/mL, median OS was 26, 21, and 11 months for men without CTCs, PSMA− CTCs, and PSMA+ CTCs. Adjusting for prior abi/enza therapy, Halabi clinical risk score, and CTC enumeration, the HRs for OS and PFS for PSMA+ CTC+ were 3.0 [95% confidence interval (CI) = 1.1–7.8] and 2.3 (95% CI = 0.9–5.8).
Conclusions:
We observed PSMA CTC heterogeneity between and within patients with mCRPC over time during abi/enza progression. CTC PSMA enumeration was adversely prognostic independent of clinical factors and disease burden. Further validation is warranted in the context of PSMA-targeted therapies.
Figure S8 from UBE2C-mediated Autophagy Inhibition via Ubiquitination of SIRT1 Contributes to Endometrial Cancer Progression
Authors: Zhao Rong , Liu Yan , Wang Ziwei , Zhang Jun , Zhang Wei , Zhou Xing , Tsedendorj Sevjid , Enkhtur Amarsanaa , Feng Dilu , Wang Hongbo
Published: April 2023

Figure S8
Figure S5 from UBE2C-mediated Autophagy Inhibition via Ubiquitination of SIRT1 Contributes to Endometrial Cancer Progression
Authors: Zhao Rong , Liu Yan , Wang Ziwei , Zhang Jun , Zhang Wei , Zhou Xing , Tsedendorj Sevjid , Enkhtur Amarsanaa , Feng Dilu , Wang Hongbo
Published: April 2023

Figure S5
Supplemental Figure 1 from USP10 Regulates ZEB1 Ubiquitination and Protein Stability to Inhibit ZEB1-Mediated Colorectal Cancer Metastasis
Authors: Sun Lei , Yu Jia , Guinney Justin , Qin Bo , Sinicrope Frank A.
Published: April 2023

Schematic Model. Constitutive activation of the RAS/RAS/MEK/ERK axis phosphorylates the deubiquitinase USP10 at serine 236, and this phosphorylation induces the dissociation of USP10 from ZEB1 resulting in stabilization of ZEB1.
Data from UBE2C-mediated Autophagy Inhibition via Ubiquitination of SIRT1 Contributes to Endometrial Cancer Progression
Authors: Zhao Rong , Liu Yan , Wang Ziwei , Zhang Jun , Zhang Wei , Zhou Xing , Tsedendorj Sevjid , Enkhtur Amarsanaa , Feng Dilu , Wang Hongbo
Published: April 2023

Abstract
Recent studies have shown that autophagy plays an important role in gynecologic tumors, and ubiquitin modification of autophagy regulatory components is essential to regulate autophagic flux. In this study, we found that the ubiquitin-conjugating enzyme E2C (UBE2C) affects endometrial cancer cell apoptosis and proliferation by inhibiting autophagy. Electron microscopy observation of cell ultrastructure and experimental biochemical analysis showed that endometrial cancer cells with UBE2C expression knocked down display typical autophagic characteristics. Cells were cotreated with the autophagy pharmacologic inhibitors chloroquine and/or bafilomycin A1, and mRFP-GFP-LC3 assays were performed to monitor autophagic flux and determine whether UBE2C suppresses the autophagy program. Investigation of the corresponding mechanism by which UBE2C inhibits autophagy revealed that UBE2C induces K48-linked SIRT1 ubiquitination and promotes ubiquitination-dependent degradation of SIRT1, subsequently reducing H4K16 deacetylation levels and epigenetically inhibiting the expression of autophagy-related genes. The results of cell counting kit-8, Hoechst staining, and immunofluorescence assays further indicated that deletion of the autophagy-related gene BECN1 significantly attenuates UBE2C knockdown–induced cell apoptosis. Moreover, overexpression of UBE2C promoted tumor growth in the xenograft mice model. While, the introduction of rapamycin, an agonist of autophagy, successfully reversed tumor growth and apoptosis inhibition mediated by UBE2C overexpression in vitro and in vivo. Taken together, our results suggested that UBE2C-mediated ubiquitination and degradation of SIRT1 contribute to the malignant progression of endometrial cancer through epigenetic inhibition of autophagy.
Implications:
Our study highlights the tumorigenic role and regulatory mechanism of UBE2C in endometrial cancer; UBE2C inhibits endometrial cancer cell apoptosis through autophagy-related mechanisms and our findings provide new insights into the treatment of endometrial cancer.
Data from USP10 Regulates ZEB1 Ubiquitination and Protein Stability to Inhibit ZEB1-Mediated Colorectal Cancer Metastasis
Authors: Sun Lei , Yu Jia , Guinney Justin , Qin Bo , Sinicrope Frank A.
Published: April 2023

Abstract
Zinc finger E-box–binding homeobox 1 (ZEB1) is a transcription factor that can promote tumor invasion and metastasis by inducing epithelial-to-mesenchymal transition (EMT). To date, regulation of ZEB1 by RAS/RAF signaling remains unclear, and few studies have examined posttranslation modification of ZEB1, including its ubiquitination. In human colorectal cancer cell lines with RAS/RAF/MEK/ERK activation, an interaction of ZEB1 with the deubiquitinase ubiquitin-specific protease 10 (USP10) was identified whereby USP10 modifies ZEB1 ubiquitination and promotes its proteasomal degradation. Regulation of the USP10–ZEB1 interaction by MEK–ERK signaling was shown whereby constitutive activation of ERK can phosphorylate USP10 at Ser236 to impair its interaction with ZEB1 and enable ZEB1 protein stabilization. Stabilized ZEB1 was shown to promote colorectal cancer metastatic colonization in a mouse tail vein injection model. Conversely, MEK–ERK inhibition blocked USP10 phosphorylation and enhanced the USP10–ZEB1 interaction shown to suppress ZEB1-mediated tumor cell migration and metastasis. In conclusion, we demonstrate a novel function of USP10 in the regulation of ZEB1 protein stability and its ability to mediate tumor metastasis in a preclinical model.
Implications:
The MEK–ERK-regulated interaction of USP10 with ZEB1 can promote the proteasomal degradation of ZEB1 and thereby suppress its demonstrated ability to mediate tumor metastasis.
Supplementary Figures from Myeloid-Derived Suppressor–Cell Dynamics Control Outcomes in the Metastatic Niche
Authors: Kreger Jesse , Roussos Torres Evanthia T. , MacLean Adam L.
Published: April 2023

Supplementary Figures
Supplementary Tables from SWI/SNF Complex Genomic Alterations as a Predictive Biomarker for Response to Immune Checkpoint Inhibitors in Multiple Cancers
Authors: Wang Di , Wang Jianchao , Zhou Dongmei , Wu Zhixian , Liu Wei , Chen Yanping , Chen Gang , Zhang Jing
Published: April 2023

Supplementary Tables S1-S12
Supplementary Figures from SWI/SNF Complex Genomic Alterations as a Predictive Biomarker for Response to Immune Checkpoint Inhibitors in Multiple Cancers
Authors: Wang Di , Wang Jianchao , Zhou Dongmei , Wu Zhixian , Liu Wei , Chen Yanping , Chen Gang , Zhang Jing
Published: April 2023

Supplementary Figures S1-S5
Data from Myeloid-Derived Suppressor–Cell Dynamics Control Outcomes in the Metastatic Niche
Authors: Kreger Jesse , Roussos Torres Evanthia T. , MacLean Adam L.
Published: April 2023

Abstract
Myeloid-derived suppressor cells (MDSC) play a prominent role in the tumor microenvironment. A quantitative understanding of the tumor–MDSC interactions that influence disease progression is critical, and currently lacking. We developed a mathematical model of metastatic growth and progression in immune-rich tumor microenvironments. We modeled the tumor–immune dynamics with stochastic delay differential equations and studied the impact of delays in MDSC activation/recruitment on tumor growth outcomes. In the lung environment, when the circulating level of MDSCs was low, the MDSC delay had a pronounced impact on the probability of new metastatic establishment: blocking MDSC recruitment could reduce the probability of metastasis by as much as 50%. To predict patient-specific MDSC responses, we fit to the model individual tumors treated with immune checkpoint inhibitors via Bayesian parameter inference. We reveal that control of the inhibition rate of natural killer (NK) cells by MDSCs had a larger influence on tumor outcomes than controlling the tumor growth rate directly. Posterior classification of tumor outcomes demonstrates that incorporating knowledge of the MDSC responses improved predictive accuracy from 63% to 82%. Investigation of the MDSC dynamics in an environment low in NK cells and abundant in cytotoxic T cells revealed, in contrast, that small MDSC delays no longer impacted metastatic growth dynamics. Our results illustrate the importance of MDSC dynamics in the tumor microenvironment overall and predict interventions promoting shifts toward less immune-suppressed states. We propose that there is a pressing need to consider MDSCs more often in analyses of tumor microenvironments.
Supplementary Figure S7 from Mitochondrial metabolism drives low-density lipoprotein-induced breast cancer cell migration
Authors: Nóbrega-Pereira Sandrina , Santos Francisco , Santos Miguel Oliveira , Serafim Teresa L. , Patrícia Lopes Ana , Coutinho Diogo , Carvalho Filipa S. , Domingues Rosário M. , Domingues Pedro , Bernardes de Jesus Bruno , Morais Vanessa A , Dias Sérgio
Published: April 2023

¬Reactive oxygen species formation downstream of mitochondria fatty acid entry is required for LDL-induced migration of breast cancer cells. Related to Fig. 6
Supplementary Figure S1 from Structurally Complex Osteosarcoma Genomes Exhibit Limited Heterogeneity within Individual Tumors and across Evolutionary Time
Authors: Rajan Sanjana , Zaccaria Simone , Cannon Matthew V. , Cam Maren , Gross Amy C. , Raphael Benjamin J. , Roberts Ryan D.
Published: April 2023

Cell count and fraction of clones
A Small-for-Gestational-Age Infant with MIRAGE Syndrome Who Developed Heat Stroke and Rhabdomyolysis due to Severe Temperature Instability
Authors: Saito Kana , Nakagawa Ryota , Narumi Satoshi , Ohashi Hirofumi , Ishiguro Akiko , Kabe Kazuhiko
Published: April 2023

MIRAGE syndrome is characterized by myelodysplasia, infection, restriction of growth, adrenal hypoplasia, genital phenotypes, and enteropathy. This report describes heat stroke and rhabdomyolysis caused by anhidrosis as a symptom of MIRAGE syndrome in a small-for-gestational-age (SGA) female neonate born at 32 weeks of gestation (birth weight, 911 g [−3.8 SD]). She developed severe temperature instability with anhidrosis, growth failure, mild developmental delay, hypothyroidism, and intractable enteropathy. On day 156, her temperature reached 42.0°C; her fever persisted for 2 h with prolonged irritability. Her serum creatine kinase level increased to a peak value of 12,716 (normal range, 43–321) IU/L. The clinical feature was diagnosed as rhabdomyolysis caused by heat stroke, which resulted from physical exertion with anhidrosis. Her SAMD9 variant was c.2945G>A, p. (Arg982His). Neonatologists should be aware of MIRAGE syndrome as a differential diagnosis of SGA with temperature instability.