bluuebunny/crossref_metadata_2025 huggingface dataset:
Birds of the Burn: Avian Community and Functional Guild Variation Five Years Post-Fire in Warm–Dry Mixed Conifer, Southwest Colorado
Authors: Scott Luke A. , Korb Julie E.
Published: February 2024

Birds contribute to the trophic interactions within mixed conifer ecosystems and provide a suite of services, such as nutrient transport, seed dispersal, habitat creation, and insect regulation. Avian communities vary in response to the structure and composition of their habitat, which may be drastically altered by fire, the predominant disturbance of western mixed conifer forests. We conducted avian point count surveys during the peak breeding season, five years post-fire, across four burn severities (unburned, low, moderate, and high) within the 416 Fire perimeter, a 55,000-acre mixed-severity fire that burned near Durango, Colorado in 2018. Avian communities in each burn severity were evaluated for richness, diversity, differentiation, indicator species, and functional guild composition. Species assemblages were significantly different across all burn severities, excluding the low to moderate areas comparison, with differentiation driven by live tree and snag density. Avian species’ richness and diversity were not significantly different across burn severities, highlighting the importance of utilizing multivariate community analysis. Unburned and high-burn areas had significant variation in functional guilds and numerous indicator species. This study provides evidence of avian community differentiation by burn severity, suggesting that management practices promoting heterogenous stand structure in warm–dry mixed conifer will positively influence avian biodiversity.
Estimation of Return Levels with Long Return Periods for Extreme Sea Levels by the Average Conditional Exceedance Rate Method
Authors: Rydén Jesper
Published: February 2024

Estimation of so-called return levels for environmental extremes is of importance for risk assessment. A particular challenge is to find estimates corresponding to long return periods, as uncertainties in the form of confidence intervals became too wide for practical use when applying conventional methodology where large portions of data are not used. A recently proposed technique, the Average Conditional Exceedance Rate (ACER), makes effective use of all available data. For risk analysis related to nuclear infrastructure, usually located along a coastline, extreme sea levels are of concern. We demonstrate, for measurements of the sea level along the Swedish coast at locations close to nuclear power plants, that the methodology results in considerably shorter confidence intervals compared to conventional approaches.
Examining the Relationship between Rainfall, Runoff, and Turbidity during the Rainy Season in Western Japan
Authors: Al Sawaf Mohamad Basel , Kawanisi Kiyosi , Bahreinimotlagh Masoud
Published: February 2024

Given the changing climate, understanding the recent variability in large-scale rainfall patterns is a crucial task in order to better understand the underlying hydrological processes that occur within a watershed. This study aims to investigate how rainfall events in western Japan have changed due to climate change and how these changes have affected runoff–turbidity dynamics during the rainy season. To address the research objectives, we analyzed two decades of precipitation records in the Gōno River watershed and examined the associated runoff–turbidity dynamics during floods using turbidity–discharge (T-Q) loops, quantified using an enhanced hysteresis index. Our findings revealed a kind of intense rainfall event occurring every 3 to 4 years. Additionally, spatial pentad analysis showed varying intensities of accumulated precipitation, indicating that extreme rainfall is not confined to a specific spatial zone. Regarding turbidity–discharge behavior, we found that clockwise hysteresis patterns were caused by sediment sources from near-channel areas, while anticlockwise patterns were caused by soil erosion from nearby areas. Another notable finding was that turbidity peaks during floods may represent the earlier (or later) arrival of turbid water from distant upstream sources due to intense precipitation. One of the key challenges in quantifying hysteresis patterns is that there is no agreed-upon definition for how to determine the start and end of a flood event. This can lead to bias in the quantification of these patterns.
Geomechanical Characterization of the Rock Mass along a Deep Vertical Borehole
Authors: Mandaglio Maria Clorinda
Published: February 2024

Deep vertical boreholes play a crucial role in underground exploration, resource extraction such as geothermal energy extraction, oil and gas exploration, underground waste storage and various underground engineering applications. The geomechanical properties of the rocks surrounding these boreholes are essential for designing safe, efficient drilling operations, for using adequate technologies and equipment and for providing mitigation measurements. Specifically, when the excavations are performed inside in-depth, extremely fractured and weathered rocks, the identification of zones more susceptible to crossing is a primary goal. This paper presents a thorough investigation into the rock masses surrounding a deep vertical borehole that involved the collection of core samples from the deep vertical borehole, laboratory testing, in situ tests and the application of geomechanical models to characterize the crossed rock masses. After a lithological and structural description of the rock masses and a description of the methodology used for their characterization, this paper focuses on the geomechanical parameterization of the rock mass using the uniaxial compressive strength of the intact rock (σci) and the Geological Strength Index (GSI). The obtained findings highlight the extreme variability in the depth of the geomechanical parameters of crossed rocks, which decreased with the depth. This methodology can be used to characterize rock masses along other deep boreholes, for which there is a lack of research, and to define the most problematic zones for underground crossing where different support works must be designed.
An Evaluation of the Efficacy and Safety of TAMIXAM®, Based on Hyaluronic Acid and Tamarind Seed Extract, for Esophageal Mucosal Protection from Acid Insult
Authors: Motta Marisa Francesca , Pulitano Giuseppe , Bagnulo Antonino , Buriani Giampaolo , Di Maio Umberto , Amone Fabio , Nobile Vincenzo , Malivindi Rocco
Published: February 2024

TAMIXAM® is a novel technology that combines hyaluronic acid and tamarind seed extract in its formulation. It is designed to protect the esophageal mucosa by creating a barrier through its filmogenic properties. The aim of this study is to evaluate the safety and efficacy of this technology through mucoadhesion tests, a cell viability assay, TEER measurements, and morphological analysis on reconstructed esophageal mucosa exposed to 10% hydrochloric acid before and after treatment. The mucoadhesion test highlighted the synergistic bioadhesive effect of the technology’s components. Cell viability assays revealed the substantial mucoprotective and barrier effects of the technology, preserving tissue viability when applied before exposure to acid insult. A morphological analysis illustrated TAMIXAM®’s efficacy in countering acid-induced damage, reducing erosion, necrosis, and tissue degeneration compared to the positive control, both pre- and post-acid insult. An evaluation of epithelial integrity through TEER measurements indicated a minimal reduction in tissues treated with the invention before acid exposure, demonstrating its ability to maintain epithelial integrity in the presence of an acid insult. However, this effect was less pronounced in tissues treated with the technology after the acid insult, implying a potential partial recovery of epithelial integrity. Furthermore, comprehensive in vitro and in vivo studies supported the safety profile of the invention. In conclusion, TAMIXAM® emerged as a compelling solution, providing enhanced mechanical action to maintain epithelial balance and shield the esophageal mucosa from acid-induced damage.
Deep Learning Enhanced Multisensor Data Fusion for Building Assessment Using Multispectral Voxels and Self-Organizing Maps
Authors: Raimundo Javier , Medina Serafin Lopez-Cuervo , Mata Julian Aguirre de , Herrero-Tejedor Tomás Ramón , Priego-de-los-Santos Enrique
Published: February 2024

Efforts in the domain of building studies involve the use of a diverse array of geomatic sensors, some providing invaluable information in the form of three-dimensional point clouds and associated registered properties. However, managing the vast amounts of data generated by these sensors presents significant challenges. To ensure the effective use of multisensor data in the context of cultural heritage preservation, it is imperative that multisensor data fusion methods be designed in such a way as to facilitate informed decision-making by curators and stakeholders. We propose a novel approach to multisensor data fusion using multispectral voxels, which enable the application of deep learning algorithms as the self-organizing maps to identify and exploit the relationships between the different sensor data. Our results indicate that this approach provides a comprehensive view of the building structure and its potential pathologies, and holds great promise for revolutionizing the study of historical buildings and their potential applications in the field of cultural heritage preservation.
An Exploration of Safety Measures in Hydrogen Refueling Stations: Delving into Hydrogen Equipment and Technical Performance
Authors: Genovese Matteo , Blekhman David , Fragiacomo Petronilla
Published: February 2024

The present paper offers a thorough examination of the safety measures enforced at hydrogen filling stations, emphasizing their crucial significance in the wider endeavor to advocate for hydrogen as a sustainable and reliable substitute for conventional fuels. The analysis reveals a wide range of crucial safety aspects in hydrogen refueling stations, including regulated hydrogen dispensing, leak detection, accurate hydrogen flow measurement, emergency shutdown systems, fire-suppression mechanisms, hydrogen distribution and pressure management, and appropriate hydrogen storage and cooling for secure refueling operations. The paper therefore explores several aspects, including the sophisticated architecture of hydrogen dispensers, reliable leak-detection systems, emergency shut-off mechanisms, and the implementation of fire-suppression tactics. Furthermore, it emphasizes that the safety and effectiveness of hydrogen filling stations are closely connected to the accuracy in the creation and upkeep of hydrogen dispensers. It highlights the need for materials and systems that can endure severe circumstances of elevated pressure and temperature while maintaining safety. The use of sophisticated leak-detection technology is crucial for rapidly detecting and reducing possible threats, therefore improving the overall safety of these facilities. Moreover, the research elucidates the complexities of emergency shut-off systems and fire-suppression tactics. These components are crucial not just for promptly managing hazards, but also for maintaining the station’s structural soundness in unanticipated circumstances. In addition, the study provides observations about recent technical progress in the industry. These advances effectively tackle current safety obstacles and provide the foundation for future breakthroughs in hydrogen fueling infrastructure. The integration of cutting-edge technology and materials, together with the development of upgraded safety measures, suggests a positive trajectory towards improved efficiency, dependability, and safety in hydrogen refueling stations.
Antimicrobial Efficacy of a Portable UV-C-Based Coating Activation Device against Candida albicans Biofilm and SARS-CoV-2 as an Additional Feature: An In Vitro Study
Authors: Putra Wigianto Adityakrisna Yoshi , Watanabe Megumi , Iwawaki Yuki , Goto Takaharu , Otsuki Tamaki , Ichikawa Tetsuo
Published: February 2024

This in vitro study aimed to confirm the effect of the additional features of Kirei Keep Light (KKL), a commercial UV-C irradiation system that was originally created for coating the surface of removable dentures with photoreactive 2-methacryloyloxyethyl phosphorylcholine (MPC), on the antimicrobial efficacy aspect against Candida albicans biofilm on a denture base material (PMMA) and SARS-CoV-2. Materials and Methods: The antimicrobial efficacy on C. albicans biofilm was evaluated through quantitative (CFU) and qualitative (SEM images) analysis of three groups: no treatment (control), KKL, and immersion in a disinfectant solution, MCAE. The quantitative evaluation on SARS-CoV-2 was performed by comparing the untreated (control) group and the KKL group. Results: In comparison with the control group (2.39 × 106 CFU/mL), KKL irradiation resulted in a 91.01% reduction in C. albicans biofilm (2.15 × 105 CFU/mL), whereas for the MCAE group, this reduction was 99.98% (4.64 × 102 CFU/mL). The SEM image results also corroborate the CFU results, which showed that the fewest clean surfaces were found in the control, and this gradually increased with KKL and MCAE. SARS-CoV-2 inhibition, indicated by its TCID50 value, demonstrated that KKL almost completely inhibited SARS-CoV-2 infection and replication (99.99% reduction). Conclusion: KKL possesses antimicrobial efficacy on C. albicans biofilm on PMMA and SARS-CoV-2.
Carcinogenic Chemicals in Occupational Settings: A Tool for Comparison and Translation between Different Classification Systems
Authors: Zellino Carolina , Spinazzè Andrea , Borghi Francesca , Campagnolo Davide , Fanti Giacomo , Keller Marta , Carminati Alessio , Rovelli Sabrina , Cattaneo Andrea , Cavallo Domenico Maria
Published: February 2024

In the European Union, Occupational Safety and Health legislation generally refers to European Regulation (CE) n. 1272/2008 to define and classify carcinogens of concern for occupational risk assessment and exposure assessment. In Europe, the current reference is Directive (UE) 2022/431, regarding carcinogen, mutagen, and reprotoxic agent (CMR) exposure. However, at the worldwide level, different classification approaches are used to establish carcinogenicity of substances and it is often difficult to compare the classifications of carcinogenicity (CoCs) proposed by different international bodies. This study aims to investigate a list of carcinogens of concern in occupational settings based on the CLP (Classification Labelling Packaging) CoC and to create a tool that allows a rapid translation–comparison of some international CoCs with the reference one. CoCs proposed by various sources were consulted and used to apply a translation method, to favor an alignment of different CoCs according to a reference. Results outlined that, considering diverse sources, CoCs can result in different classifications of the same chemicals. Overall, this may have implications for the hazard assessment process, which is the base of risk assessment. The proposed tool is expected to help risk assessors in the occupational field when it is needed to have a comparison with different CoC systems.
Assessment of Surgical Difficulty in Patients with Rectal Cancer—The Impact of Pelvimetry
Authors: Stuart João , Santos Pedro Miguel Dias dos , Pereira Carlos Costa , Martins Sandra F.
Published: February 2024

Background: Low-quality tumoral surgical excision is the major relapse factor in rectal cancer. If the surgery is highly difficult, the quality of the resection might be compromised. In the literature, it is described how low pelvic dimensions can make this type of surgery difficult. The main aim was to study the influence of pelvic measures in surgical difficulty on the patients submitted to tumoral surgical resection with curative intent. Methods: A retrospective, observational and analytic study was conducted. A total of 73 patients over a period of 3 years were included. Demographic and surgical data, as well as measurements of the pelvis taken from MRI, were collected. An univariate and multivariate analysis was performed. Results: 11 (15.1%) patients were classified as having highly difficult surgeries. All 11 patients were male. Significant differences were found between groups regarding gender (p = 0.013), transverse diameter of the pelvis (p < 0.001), interspinal distance (p = 0.014) and intertuberous distance (p < 0.001). The logistic regression revealed that a small transverse diameter (O.R. 0.919, 95% I.C. 0.846–0.999, p = 0.047) increases the degree of difficulty of the surgery. Conclusions: Male patients with a small pelvic measurement deserve a thorough surgical plan that predicts a quality resection.
A Review of Perspectives on Developing Floating Wind Farms
Authors: Maktabi Mohamed , Rusu Eugen
Published: February 2024

Floating wind is becoming an essential part of renewable energy, and so highlighting perspectives of developing floating wind platforms is very important. In this paper, we focus on floating wind concepts and projects around the world, which will show the reader what is going on with the projects globally, and will also provide insight into the concepts and their corresponding related aspects. The main aim of this work is to classify floating wind concepts in terms of their number and manufacturing material, and to classify the floating wind projects in terms of their power capacity, their number, character (if they are installed or planned) and the corresponding continents and countries where they are based. We will classify the corresponding additional available data that corresponds to some of these projects, with reference to their costs, wind speeds, water depths, and distances to shore. In addition, the floating wind global situation and its corresponding aspects of relevance will be also covered in detail throughout the paper.
Predicting the Dynamic Parameters for Milling Thin-Walled Blades with a Neural Network
Authors: Li Yu , Ding Feng , Wang Dazhen , Tian Weijun , Zhou Jinhua
Published: February 2024

Accurately predicting the time-varying dynamic parameters of a workpiece during the milling of thin-walled parts is the foundation of adaptively selecting chatter-free machining parameters. Hence, a method for accurately and quickly predicting the time-varying dynamic parameters for milling thin-walled parts is proposed, which is based on the shell FEM and a three-layer neural network. The time-dependent dynamics of the workpiece can be calculated using the FEM by obtaining the geometrical parameters of the arc-faced junctions within the discrete cells of the initial and machined workpiece. It is unnecessary to re-divide the mesh cells of the thin-walled parts at each cutting position, which enhances the computational efficiency of the workpiece dynamics. Meanwhile, in comparison with the three-dimensional cube elements, the shell elements can reduce the number of degrees of freedom of the FEM model by 74%, which leads to the computation of the characteristic equation that is about nine times faster. The results of the modal test show that the maximum error of the shell FEM in predicting the natural frequency of the workpiece is about 4%. Furthermore, a three-layer neural network is constructed, and the results of the shell FEM are used as samples to train the model. The neural network model has a maximum prediction error of 0.409% when benchmarked against the results of the FEM. Furthermore, the three-layer neural network effectively enhances computational efficiency while guaranteeing accuracy.
Graphene Oxide Chemical Refining Screening to Improve Blood Compatibility of Graphene-Based Nanomaterials
Authors: Pieretti Fabio , Moretto Alessandro , Papini Emanuele , Tavano Regina
Published: February 2024

Graphene oxide (GO) nanoparticles, due to their favorable water solubility, compared to graphene (GA), are a hot research topic in biomedical and pharmaceutical research. However, GO clinical translation may be complicated by its high surface/volume ratio enhancing the interaction with human blood components. In fact, GO’s bi-dimensional nature and strong negative charge may lead to severe biological effects, such as thrombogenicity and immune cell activation. This study explores the impact of further GO surface chemical modulation on major adverse effects: blood plasma coagulation and hemolysis. To this aim, we refined GO nanoparticles by fine-tuned reduction chemistry, esterification and introduction of negative or positive charges. With this approach, we were able to mitigate plasma coagulation and hemolysis at variable degrees and to identify GO derivatives with improved biocompatibility. This opens the door to the progress of graphene-based nanotheranostic applications.
Etiology, Clinical Approach, and Therapeutic Consequences of Hyponatremia
Authors: Spasovski Goce
Published: February 2024

A perturbation in the water balance rather than any change in salt content is the main cause of hyponatremia, the most frequent electrolyte abnormality, defined as a serum sodium concentration <135 mEq/L. Hyponatremia may be divided between mild (Na > 120 mEq/L) or severe (Na < 120 mEq/L) hyponatremia, and is most frequently observed in elderly ICU hospitalized patients. Based on tonicity, hyponatremia may be hypotonic (a decreased concentration of the solute), isotonic, and hypertonic (falsely low sodium). According to the volume of extracellular fluid (ECF), hyponatremia is further divided among hypovolemic, euvolemic, or hypervolemic hyponatremia. Finally, hyponatremia may develop rapidly as acute (<48 h), usually with severe symptoms, or slowly as chronic hyponatremia, usually being asymptomatic or with mild symptoms. Acute severe hyponatremia presents with severe CNS problems, increased hospitalization rates, and mortality. The treatment with 3% sodium chloride and a 100 mL IV bolus based on severity and persistence of symptoms needs careful monitoring. A non-severe hyponatremia may be treated with oral urea. In asymptomatic mild hyponatremia, an adequate solute intake with an initial fluid restriction of 500 mL/d adjusted according to the serum sodium levels is preferred. Vaptans could be considered in patients with high ADH activity regardless of whether they are euvolemic or hypervolemic. In general, the treatment of hyponatremia should be based on the underlying cause, the duration and degree of hyponatremia, the observed symptoms, and volume status of patient.
Συνουσία in Late Antique Neoplatonic Schools: A Concept between Social History, History of Education and History of Philosophy
Authors: Alviz Fernández Marco , Hernández de la Fuente David
Published: February 2024

It is well studied that some Pythagorean principles lied at the foundations of the Late Antique Neoplatonic School. The main reason for that conclusion to be drawn is the two biographies of the Samian sage written by the Neoplatonic philosophers Porphyry of Tyre and Iamblichus of Chalcis. Accordingly, the archetypical image of Pythagoras became a major ideal for which every pagan philosopher aimed in Late Antiquity. Henceforth, masters and their disciple circles comprised a micro-society which can reasonably be analyzed as a whole. Suffice it to say that they were small and cohesive charismatic communities whose isolation from the outside world aroused a living harmony from which emerged long-standing emotional bonds. Consequently, the Pythagorically rooted κοινός βίος (Iambl. Vit. Pyth. 6.29: τὸ λεγόμενον κοινοβίους) can easily be ascertained in the biographical literature around the philosophical schools from Plotinus to Damascius (cf. Porph. Vit. Plot. 18.6-14; Procl. In Resp. passim). It is a way of life in common which was also known at the old Athenian Academy (according to Plato’s only explicit reference to Pythagoras (Resp. 600a-b: Πυθαγόρειον τρόπον τοῦ βίου) and has sometimes been defined even as “coenobitic”, in analogy with other contemporary phenomena. But from our point of view, it can be better understood through an analysis of the concept of συνουσία—that is, the meetings of philosophers with their companions (ἑταῖροι) in a specific place which turned into a sort of spiritual household. With this contribution, we aim at focusing on the redefinition of the Neoplatonic συνουσίαι as a legacy of the Platonic notion of συνουσία, stemming from Pythagorean κοινόβιοι. To sum up, we will revise this issue and the state of the art, with the redefinition of Late Antique συνουσία as a terminus technicus in the biographic literature around the Neoplatonic Schools, aiming at opening new paths for the understanding of the Pythagorean–Platonic heritage in Late Antiquity.
RETRACTED: Impact of High-Frequency Traveling-Wave Magnetic Fields on Low-Conductivity Liquids: Investigation and Potential Applications in the Chemical Industry
Authors: Cui Xinyu , Na Xianzhao , Wang Xiaodong , Ernst Roland , Yves Fautrelle
Published: February 2024

High-frequency traveling-wave magnetic fields refer to alternating magnetic fields that propagate through space in a wave-like manner at high frequencies. These magnetic fields are characterized by their ability to generate driving forces and induce currents in conductive materials, such as liquids or metals. This article investigates the application and approaches of a unique form of high-frequency traveling-wave magnetic fields to low-conductivity liquids with conductivity ranging from 1 to 102 S/m. Experiments were conducted using four representative electrolytic solutions commonly employed in the chemical industry: sulfuric acid (H2SO4), sodium hydroxide (NaOH), sodium chloride (NaCl), and ionic liquid ([Bmim]BF4). The investigation focuses on the impact of high-frequency magnetic fields on these solutions at the optimal operating point of the system, considering the effects of Joule heating. The findings reveal that the high-frequency traveling magnetic field exerts a significant volumetric force on all four low-conductivity liquids. This technology, characterized by its non-contact and pollution-free nature, high efficiency, large driving volume, and rapid driving speeds (up to several centimeters per second), also provides uniform velocity distribution and notable thermal effects. It holds considerable promise for applications in the chemical industry, metallurgy, and other sectors where enhanced three-phase transfer processes are essential.
High-Strength Copper/Silver Alloys Processed by Cold Spraying for DC and Pulsed High Magnetic Fields
Authors: Tardieu Simon , Idrir Hanane , Verdy Christophe , Jay Olivier , Ferreira Nelson , Debray François , Joulain Anne , Tromas Christophe , Thilly Ludovic , Lecouturier-Dupouy Florence
Published: February 2024

High-strength, high-conductivity copper/silver-alloyed materials were prepared by cold-spray (CS) manufacturing. For DC high-field application at room temperature, bulk Cu/Ag (5% vol. Ag) alloys with high mechanical properties and high electrical conductivity can be obtained by CS and post-heat treatments. For pulsed-field application at liquid nitrogen temperature, bulk Cu/Ag (5% vol. Ag) alloys serve as precursors for room-temperature wire drawing. The Cu/Ag-alloyed bulk CS deposit presents a high yield strength of about 510 MPa with a corresponding electrical resistivity of 1.92 µΩ·cm (at 293 K). The Cu/Ag-alloyed wires show a very high ultimate tensile strength (1660 MPa at 77 K or 1370 MPa at 293 K) and low electrical resistivity (1.05 µΩ·cm at 77 K or 2.56 µΩ·cm at 293 K). Microstructural studies via STEM allow us to understand this very high level of mechanical strength. The results evidence that materials developed by CS exhibit very high mechanical properties compared to materials prepared by other routes, due to the high velocity of the deposited particles, which leads to high initial deformation rates and specific microstructural features.
Evaluation of the Levels of Selected Cytokines and Their Possible Influence on the Development of Cardiovascular and Pulmonary Complications in Patients after COVID-19
Authors: Stanjek-Cichoracka Anita , Niedziela Jacek T. , Łaszewska Anna , Mędrala Zofia , Nowowiejska-Wiewióra Alicja , Kaczmarski Jacek , Grzanka Alicja , Gąsior Mariusz
Published: February 2024

Background and Objectives: The aim of this study was to evaluate the levels of selected cytokines and their possible influence on the development of cardiovascular and pulmonary complications in patients hospitalized at the Silesian Centre for Heart Disease in Zabrze after having undergone COVID-19. Materials and methods: The study included 76 randomly selected patients from the SILCOVID-19 database. The median time from symptom onset to the study visit was 102 (86–118) days. The median age of the study group was 53 (44–60) years. Assays of a panel of 30 cytokines were carried out in the serum of patients on a Luminex100 platform using the Milliplex MAP kit from Merck KGaA Germany. Results: There were no statistically significant differences in most of the cytokines analyzed between patients with confirmed or excluded lung lesions or cardiac abnormalities. Additionally, no statistically significant differences in cytokine concentrations according to gender, age, comorbidity of diabetes, renal disease, hypertension, increased risk of thrombotic disease, or psychological disorders were demonstrated. There were high concentrations of cytokines such as platelet-derived growth actor-AA (PDGF-AA), monocyte chemoattractant protein-1 (MCP-1), monokine-induced gamma interferon (MIG), and vascular endothelial growth factor-A (VEGF-A). Conclusions: No direct impact of the dependencies between a panel of cytokines and the incidence of cardiovascular and pulmonary complications in patients hospitalized at the Silesian Centre for Heart Disease in Zabrze after having undergone COVID-19 was demonstrated. The demonstration of high levels of certain cytokines (PDGF-AA, VEGF, MIG, and IP10) that are of significance in the development of many lung diseases, as well as cytokines (MCP-1) that influence the aetiopathogenesis of cardiovascular diseases seems to be highly concerning in COVID-19 survivors. This group of patients should receive further monitoring of these cytokine levels and diagnostic imaging in order to detect more severe abnormalities as early as possible and administer appropriate therapy.
The Serotonin-Mediated Anti-Allodynic Effect of Yokukansan on Paclitaxel-Induced Neuropathic Pain
Authors: Yokomi Hiroshi , Kato Takahiro , Narasaki Soshi , Kamiya Satoshi , Taguchi Shima , Horikawa Yosuke T. , Tsutsumi Yasuo M.
Published: February 2024

Refractory peripheral neuropathy can occur as a side effect in 60–70% of patients receiving Paclitaxel (PTX). Yokukansan (YKS) is a Japanese herbal medicine reported to have analgesic properties for entrapment nerve injuries. Therefore, we investigated the anti-allodynic effect of Yokukansan on Paclitaxel-induced neuropathic pain. All experiments used 6-week-old male Sprague Dawley rats. Mechanical allodynia was evaluated using a dynamic plantar aesthesiometer. A mobile touch-stimulator unit applied progressively increasing force to the mid-plantar region of the hind paw in a vertical direction until the animal withdrew its paw. This was carried out before the Paclitaxel administration and during the first, second, third, and fourth weeks. Using a rat model of PTX-induced neuropathic pain (PTX rat), we injected PTX (intraperitoneally, 2 mg/kg) five times every 2 days. Using the dynamic plantar test, we evaluated the anti-allodynic effect of YKS (orally administered, 1 g/kg). YKS administration on a daily basis significantly enhanced the withdrawal threshold in PTX rats and reduced the expression level of activated microglia immunostaining with Iba1, a specific marker for microglia. The intrathecal administration of WAY-100635 (5-hydroxytryptamine [5-HT]1A receptor antagonist) and Ketanserin (5-HT2A/2C receptor antagonist) inhibited the protective effects of YKS. YKS exhibited an anti-allodynic effect in a rodent model of PTX-induced neuropathic pain by reducing the sensitivity to pain stimuli. These results suggest that Yokukansan may activate 5-HT receptors in the spinal cord, mediating Paclitaxel-induced neuropathic pain.
The Decorated Garden Grotto of Condes de Basto Palace in Évora, Portugal: Microbial Community Characterization and Biocide Tests for Conservation
Authors: Silva Inês , Salvador Cátia , Arantes Sílvia , Miller Ana Z. , Candeias António , Caldeira Ana Teresa
Published: February 2024

The Eugénio de Almeida Foundation’s Casa de Fresco is a historical monument of valuable historic–artistic significance, which currently reveals an assortment of biofilms due to the proliferation of microorganisms in the stone and rocaille elements. The biodeterioration in this area was studied as part of the Conservation and Restoration Project. We effectively characterized the local microbial community using modern high-throughput DNA analysis. Our results suggested the existence of a variety of lichens or lichenized fungi, including genera such as Variospora, Verrucaria, Circinaria, and Caloplaca. Furthermore, we detected several prokaryote microorganisms related to the identification of these lichens. To properly deal with this microbiological issue and avoid fungal recolonization, we evaluated available commercial antimicrobial treatments.
Sub-Surface Soil Characterization Using Image Analysis: Material Recognition Using the Grey Level Co-Occurrence Matrix Applied to a Video-CPT-Cone
Authors: Khomiak Oksana , Benndorf Jörg , Verbeek Gerald
Published: February 2024

The geotechnical characterization of the subsurface is a key requirement for most soil investigations, incl. those for reclaiming landfills and waste dumps associated with mining operations. New sensor technology, combined with intelligent analysis algorithms, allow for a faster and less expensive acquisition of the necessary information without loss of data quality. The use of advanced technologies to support and back up common site investigation techniques, such as cone penetration testing (CPT), can enhance the underground characterization process. This study aims to investigate the possibilities of image analysis for material recognition to advance the geotechnical characterization process. The grey level co-occurrence matrix (GLCM) image processing technique is used in a wide range of study fields to estimate textures, patterns and structure anomalies. This method was adjusted and applied to process the video recorded during a CPT sounding, in order to distinguish soil types by its changing surface characteristics. From the results of the video processing, it is evident that the GLCM technique can identify transitions in soil types that were captured in the video recording. This enables the prospect of image analysis not just for soil investigations, but also for monitoring of the conveyor belt in the mining field, to allow for efficient preliminary decision making, material documentation and quality control by providing information in a cost effective and efficient manner.
Dendrobium officinale Polysaccharides as a Natural Functional Component for Acetic-Acid-Induced Gastric Ulcers in Rats
Authors: Zhang Miao , Xu Liba , Chen Long , Wu Huan , Jia Li , Zhu Hua
Published: February 2024

Dendrobium officinale is an important edible and medicinal plant, with the Dendrobium officinale polysaccharide (DOP) being its primary active constituent, known for its diverse biological activities. In this study, DOP was extracted and characterized for its structural properties. The potential of DOP to ameliorate gastric ulcers (GUs) was investigated using an acetic-acid-induced GU model in rats. The results demonstrated that DOP exerted a multifaceted protective effect against GU, mitigating the deleterious impact on food intake and body weight in rats. DOP exhibited its protective action by attenuating cellular damage attributed to oxidative stress and inflammatory reactions mediated by enhanced activities of SOD, GSH, and GSH-PX, coupled with a downregulation in the expression of pro-inflammatory cytokines, including IL-1β, IL-6, and TNF-α. Furthermore, DOP effectively inhibited apoptosis in gastric mucosa cells of acetic-acid-induced GU rat models and facilitated the self-repair of damaged tissues. Remarkably, the DOP-200 and DOP-400 groups outperformed omeprazole in reducing the expression of IL-6 and malondialdehyde (MDA) in tissues, as well as IL-1β, IL-6, and TNF-α in serum. These groups also exhibited an improved expression of SOD in tissues and SOD, GSH, and GSH-PX in serum. A Western blot analysis of gastric mucosa demonstrated that the DOP-200 and DOP-400 groups significantly reduced the expression of NF-κBp65, phosphorylated NF-κBp65, FoxO3a, and Bim. The observed antagonism to GU appeared to be associated with the NF-κB cell pathway. Additionally, qRT-PCR results indicate that DOP reduced the mRNA transcription levels of IL-6, and TNF-α, which shows that the healing of GU is related to the reduction in the inflammatory reaction by DOP. However, the expression of EGF and VEGF decreased, suggesting that the mechanism of DOP inhibiting GU may not be directly related to EGF and VEGF, or there is an uncertain competitive relationship between them, so further research is needed.
Correction: Ghani et al. Multi-Dimensional Antioxidant Screening of Selected Australian Native Plants and Putative Annotation of Active Compounds. Molecules 2023, 28, 3106
Authors: Ghani Md. Ahsan , Barril Celia , Bedgood Danny R. , Burrows Geoffrey E. , Ryan Danielle , Prenzler Paul D.
Published: February 2024

In the original publication [...]
Salvianolic Acid B Significantly Suppresses the Migration of Melanoma Cells via Direct Interaction with β-Actin
Authors: Zhang Ying , Zhai Wenjuan , Fan Minqi , Wu Jinjun , Wang Caiyan
Published: February 2024

Melanoma is the most aggressive and difficult to treat of all skin cancers. Despite advances in the treatment of melanoma, the prognosis for melanoma patients remains poor, and the recurrence rate remains high. There is substantial evidence that Chinese herbals effectively prevent and treat melanoma. The bioactive ingredient Salvianolic acid B (SAB) found in Salvia miltiorrhiza, a well-known Chinese herbal with various biological functions, exhibits inhibitory activity against various cancers. A375 and mouse B16 cell lines were used to evaluate the main targets and mechanisms of SAB in inhibiting melanoma migration. Online bioinformatics analysis, Western blotting, immunofluorescence, molecular fishing, dot blot, and molecular docking assays were carried out to clarify the potential molecular mechanism. We found that SAB prevents the migration and invasion of melanoma cells by inhibiting the epithelial–mesenchymal transition (EMT) process of melanoma cells. As well as interacting directly with the N-terminal domain of β-actin, SAB enhanced its compactness and stability, thereby inhibiting the migration of cells. Taken together, SAB could significantly suppress the migration of melanoma cells via direct binding with β-actin, suggesting that SAB could be a helpful supplement that may enhance chemotherapeutic outcomes and benefit melanoma patients.
Exploring Four Atraphaxis Species: Traditional Medicinal Uses, Phytochemistry, and Pharmacological Activities
Authors: Abilkassymova Alima , Turgumbayeva Aknur , Sarsenova Lazzat , Tastambek Kuanysh , Altynbay Nazym , Ziyaeva Gulnar , Blatov Ravil , Altynbayeva Gulmira , Bekesheva Kuralay , Abdieva Gulzhamal , Ualieva Perizat , Shynykul Zhanserik , Kalykova Assem
Published: February 2024

Atraphaxis is a genus of flowering plants in the family Polygonaceae, with approximately 60 species. Species of Atraphaxis are much-branched woody plants, forming shrubs or shrubby tufts, primarily inhabiting arid zones across the temperate steppe and desert regions of Central Asia, America, and Australia. Atraphaxis species have been used by diverse groups of people all over the world for the treatment of various diseases. However, their biologically active compounds with therapeutic properties have not been investigated well. Studying the biologically active components of Atraphaxis laetevirens, Atraphaxis frutescens, Atraphaxis spinosa L., and Atraphaxis pyrifolia is crucial for several reasons. Firstly, it can unveil the therapeutic potential of these plants, aiding in the development of novel medicines or natural remedies for various health conditions. Understanding their bioactive compounds enables scientists to explore their pharmacological properties, potentially leading to the discovery of new drugs or treatments. Additionally, investigating these components contributes to preserving traditional knowledge and validating the historical uses of these plants in ethnomedicine, thus supporting their conservation and sustainable utilization. These herbs have been used as an anti-inflammatory and hypertension remedies since the dawn of time. Moreover, they have been used to treat a variety of gastrointestinal disorders and problems related to skin in traditional Kazakh medicine. Hence, the genus Atraphaxis can be considered as a potential medicinal plant source that is very rich in biologically active compounds that may exhibit great pharmacological properties, such as antioxidant, antibacterial, antiulcer, hypoglycemic, wound healing, neuroprotective, antidiabetic, and so on. This study aims to provide a collection of publications on the species of Atraphaxis, along with a critical review of the literature data. This review will constitute support for further investigations on the pharmacological activity of these medicinal plant species.
Enzymatic Synthesis of Structured Lipids Enriched with Medium- and Long-Chain Triacylglycerols via Pickering Emulsion-Assisted Interfacial Catalysis: A Preliminary Exploration
Authors: Dong Zhe , Cui Ziheng , Jin Jun , Cheng Xinyi , Wu Gangcheng , Wang Xingguo , Jin Qingzhe
Published: February 2024

Medium- and long-chain triacylglycerol (MLCT), as a novel functional lipid, is valuable due to its special nutritional properties. Its low content in natural resources and inefficient synthesis during preparation have limited its practical applications. In this study, we developed an effective Pickering emulsion interfacial catalysis system (PE system) for the enzymatic synthesis of MLCT by trans-esterification. Lipase NS 40086 served simultaneously as a catalyst and a solid emulsifier to stabilize the Pickering emulsion. Benefitting from the sufficient oil–water interface, the obtained PE system exhibited outstanding catalytic efficiency, achieving 77.5% of MLCT content within 30 min, 26% higher than that of a water-free system. The Km value (0.259 mM) and activation energy (14.45 kJ mol−1) were 6.8-fold and 1.6-fold lower than those of the water-free system, respectively. The kinetic parameters as well as the molecular dynamics simulation and the tunnel analysis implied that the oil–water interface enhanced the binding between substrate and lipase and thus boosted catalytic efficiency. The conformational changes in the lipase were further explored by FT-IR. This method could give a novel strategy for enhancing lipase activity and the design of efficient catalytic systems to produce added-value lipids. This work will open a new methodology for the enzymatic synthesis of structured lipids.
Biosynthesis, Characterization, and Biomedical Applications of Gold Nanoparticles with Cucurbita moschata Duchesne Ex Poiret Peel Aqueous Extracts
Authors: Kaval Uğur , Hoşgören Hülya
Published: February 2024

In this study, AuNPs were biosynthesized from Cucurbita moschata fruit peel extracts. Biosynthesized AuNPs exhibited maximum absorbance at a 555 nm wavelength, and XRD analysis indicated that the CM-AuNPs had a particle size of less than 100 nm and a cubic crystal structure. TEM scans revealed that the gold particles exhibited a spherical morphology, with an average size of 18.10 nm. FTIR analysis revealed strong peaks indicating the presence of functional groups involved in the reduction reactions. The surface charge of the biosynthesized AuNPs was determined to be −19.7 mV. The antibacterial and antifungal activities of AuNPs against pathogen strains were assessed by the minimum inhibitory concentration (MIC) method. The cytotoxic effects of CM-AuNPs on cancer cell lines (Sk-Ov-3, CaCo2, and A549) and healthy cell lines (HUVEC) were investigated using the MTT method. The findings indicated that AuNPs biosynthesized by the green synthesis method using C. moschata peel aqueous extract had high inhibition on the growth of pathogenic microorganisms and effective cytotoxic activity against cancerous cell lines at low doses. As a result, it can be concluded that CM-AuNPs will be eminently effective in the production of antibacterial and/or anticancer drugs in the pharmaceutical, food, and cosmetic industries.
Design of Molecularly Imprinted Polymers Using Supercritical Carbon Dioxide Technology
Authors: Furtado Ana I. , Bonifácio Vasco D. B. , Viveiros Raquel , Casimiro Teresa
Published: February 2024

The design and development of affinity polymeric materials through the use of green technology, such as supercritical carbon dioxide (scCO2), is a rapidly evolving field of research with vast applications across diverse areas, including analytical chemistry, pharmaceuticals, biomedicine, energy, food, and environmental remediation. These affinity polymeric materials are specifically engineered to interact with target molecules, demonstrating high affinity and selectivity. The unique properties of scCO2, which present both liquid– and gas–like properties and an accessible critical point, offer an environmentally–friendly and highly efficient technology for the synthesis and processing of polymers. The design and the synthesis of affinity polymeric materials in scCO2 involve several strategies. Commonly, the incorporation of functional groups or ligands into the polymer matrix allows for selective interactions with target compounds. The choice of monomer type, ligands, and synthesis conditions are key parameters of material performance in terms of both affinity and selectivity. In addition, molecular imprinting allied with co–polymerization and surface modification are commonly used in these strategies, enhancing the materials’ performance and versatility. This review aims to provide an overview of the key strategies and recent advancements in the design of affinity polymeric materials using scCO2.
Marine-Derived Bisindoles for Potent Selective Cancer Drug Discovery and Development
Authors: Xu Mengwei , Bai Zhaofang , Xie Baocheng , Peng Rui , Du Ziwei , Liu Yan , Zhang Guangshuai , Yan Si , Xiao Xiaohe , Qin Shuanglin
Published: February 2024

Marine-derived bisindoles exhibit structural diversity and exert anti-cancer influence through multiple mechanisms. Comprehensive research has shown that the development success rate of drugs derived from marine natural products is four times higher than that of other natural derivatives. Currently, there are 20 marine-derived drugs used in clinical practice, with 11 of them demonstrating anti-tumor effects. This article provides a thorough review of recent advancements in anti-tumor exploration involving 167 natural marine bisindole products and their derivatives. Not only has enzastaurin entered clinical practice, but there is also a successfully marketed marine-derived bisindole compound called midostaurin that is used for the treatment of acute myeloid leukemia. In summary, investigations into the biological activity and clinical progress of marine-derived bisindoles have revealed their remarkable selectivity, minimal toxicity, and efficacy against various cancer cells. Consequently, they exhibit immense potential in the field of anti-tumor drug development, especially in the field of anti-tumor drug resistance. In the future, these compounds may serve as promising leads in the discovery and development of novel cancer therapeutics.