bluuebunny/crossref_metadata_2025 huggingface dataset:
European Hares, Lepus europaeus, Represent a Reservoir Host for Thelazia callipaeda in Romania
Authors: Cotuțiu Vlad-Dan , Mihalca Andrei Daniel , Hołówka Katarzyna Anna , Ionică Angela Monica , Cazan Cristina Daniela , Gherman Călin Mircea
Published: October 2022

Thelaziosis caused by Thelazia callipaeda is an emerging disease in Europe. Only two reports of naturally infected lagomorphs have been published so far. The aim of this study was to evaluate the status of the Romanian populations of European brown hares, Lepus europaeus as reservoir hosts for T. callipaeda. Between November 2019 and November 2021, the eyes of 326 L. europaeus carcasses were examined for the presence of ocular parasites. Nematodes were stored in plastic vials with physiological saline, followed by morphological and molecular identification. QGis 3.20 and EpiInfoTM 7 were used for mapping and statistical analysis. Four (1.23%) hares harbored T. callipaeda infection, with a total of 84 nematodes collected (mean intensity 21 nematodes/host), with 45 males, 39 females (two sexually immature, seven with only eggs, and 30 with eggs and larvae). One specimen from each host was successfully sequenced resulting in a 100% similarity with several other sequences of T. callipaeda haplotype 1. Statistical analysis revealed no significant results. The current study represents a first report of T. callipaeda in the European brown hare in Romania, and the second in Europe, also reiterating the role of lagomorphs as reservoir hosts for this zoonotic ocular nematode.
Hyperinsulinemic Hypoglycemia Due to PMM2 Mutation in Two Siblings with Autosomal Recessive Polycystic Kidney Disease
Authors: Acharya Ratna , Upadhyay Kiran
Published: October 2022

Background: Hyperinsulinemic hypoglycemia (HH) is an important cause of persistent hypoglycemia in newborns and infants. Recently, PMM2 (phosphomannomutase 2) mutation has been associated with HH, especially in conjunction with polycystic kidney disease (PKD). PMM2 deficiency is one of the most common causes of congenital disorder of glycosylation (CDG). Renal involvement in PMM2-CDG manifests as cystic kidney disease, echogenic kidneys, nephrotic syndrome or mild proteinuria. Case Summary: Here, we describe a pair of siblings with HH associated with autosomal recessive polycystic kidney disease (ARPKD) and PMM2 mutation. Two siblings with ARPKD presented during infancy and early toddler years with severe hypoglycemia. Both had inappropriately elevated serum insulin, low β-hydroxybutyrate, a need for a high glucose infusion rate, positive glycemic response to glucagon, positive diazoxide response and PMM2 mutation. Conclusions: Although this combination of HH and PKD was recently described in patients of European descent who also had PMM2 mutation, our report is unique given that these non-consanguineous siblings were not exclusively of European descent. PMM2 mutation leading to abnormal glycosylation and causing cystic kidneys and the alteration of insulin secretion is the most likely pathogenesis of this clinical spectrum.
Synthesis and Chemopreventive Potential of 5-FU/Genistein Hybrids on Colorectal Cancer Cells
Authors: Moreno-Quintero Gustavo , Castrillón-Lopez Wilson , Herrera-Ramirez Angie , Yepes-Pérez Andrés F. , Quintero-Saumeth Jorge , Cardona-Galeano Wilson
Published: October 2022

A series of 5-FU-Genistein hybrids were synthesized and their structures were elucidated by spectroscopic analysis. The chemopreventive potential of these compounds was evaluated in human colon adenocarcinoma cells (SW480 and SW620) and non-malignant cell lines (HaCaT and CHO-K1). Hybrid 4a displayed cytotoxicity against SW480 and SW620 cells with IC50 values of 62.73 ± 7.26 µM and 50.58 ± 1.33 µM, respectively; compound 4g induced cytotoxicity in SW620 cells with an IC50 value of 36.84 ± 0.71 µM. These compounds were even more selective than genistein alone, the reference drug (5-FU) and the equimolar mixture of genistein plus 5-FU. In addition, hybrids 4a and 4g induced time- and concentration-dependent antiproliferative activity and cell cycle arrest at the S-phase and G2/M. It was also observed that hybrid 4a induced apoptosis in SW620 cells probably triggered by the extrinsic pathway in response to the activation of p53, as evidenced by the increase in the levels of caspases 3/8 and the tumor suppressor protein (Tp53). Molecular docking studies suggest that the most active compound 4a would bind efficiently to proapoptotic human caspases 3/8 and human Tp53, which in turn could provide valuable information on the biochemical mechanism for the in vitro cytotoxic response of this compound in SW620 colon carcinoma cell lines. On the other hand, molecular dynamics (MD) studies provided strong evidence of the conformational stability of the complex between caspase-3 and hybrid 4a obtained throughout 100 ns all-atom MD simulation. Molecular mechanics Poisson–Boltzmann surface area (MM-PBSA) analyses of the complex with caspase-3 showed that the interaction between the ligand and the target protein is stable. Altogether, the results suggest that the active hybrids, mainly compound 4a, might act by modulating caspase-3 activity in a colorectal cancer model, making it a privileged scaffold that could be used in future investigations.
High-altitude Hypoxia Influences the Activities of the Drug-Metabolizing Enzyme CYP3A1 and the Pharmacokinetics of Four Cardiovascular System Drugs
Authors: Zhu Junbo , Duan Yabin , Duo Delong , Yang Jianxin , Bai Xue , Liu Guiqin , Wang Qian , Wang Xuejun , Qu Ning , Zhou Yang , Li Xiangyang
Published: October 2022

(1) Background: High-altitude hypoxia has been shown to affect the pharmacokinetic properties of drugs. Although there is a high incidence of cardiovascular disease among individuals living in high-altitude areas, studies on the effect of high-altitude hypoxia on the pharmacokinetic properties of cardiovascular drugs are limited. (2) Methods: The aim of this study was to evaluate the pharmacokinetics of nifedipine, bosentan, simvastatin, sildenafil, and their respective main metabolites, dehydronifedipine, hydroxybosentan, simvastatin hydroxy acid, and N-desmethyl sildenafil, in rats exposed to high-altitude hypoxia. Additionally, the protein and mRNA expression of cytochrome P450 3A1 (CYP3A1), a drug-metabolizing enzyme, were examined. (3) Results: There were significant changes in the pharmacokinetic properties of the drugs in rats exposed to high-altitude hypoxia, as evidenced by an increase in the area under the curve (AUC) and the half-life (t1/2z) and a decrease in total plasma clearance (CLz/F). However, most of these changes were reversed when the rats returned to a normoxic environment. Additionally, there was a significant decrease in CYP3A1 expression in rats exposed to high-altitude hypoxia at both the protein and mRNA levels. (4) Conclusions: High-altitude hypoxia suppressed the metabolism of the drugs, indicating that the pharmacokinetics of the drugs should be re-examined, and the optimal dose should be reassessed in patients living in high-altitude areas.
Intrinsic Aqueous Solubility: Mechanistically Transparent Data-Driven Modeling of Drug Substances
Authors: Oja Mare , Sild Sulev , Piir Geven , Maran Uko
Published: October 2022

Intrinsic aqueous solubility is a foundational property for understanding the chemical, technological, pharmaceutical, and environmental behavior of drug substances. Despite years of solubility research, molecular structure-based prediction of the intrinsic aqueous solubility of drug substances is still under active investigation. This paper describes the authors’ systematic data-driven modelling in which two fit-for-purpose training data sets for intrinsic aqueous solubility were collected and curated, and three quantitative structure–property relationships were derived to make predictions for the most recent solubility challenge. All three models perform well individually, while being mechanistically transparent and easy to understand. Molecular descriptors involved in the models are related to the following key steps in the solubility process: dissociation of the molecule from the crystal, formation of a cavity in the solvent, and insertion of the molecule into the solvent. A consensus modeling approach with these models remarkably improved prediction capability and reduced the number of strong outliers by more than two times. The performance and outliers of the second solubility challenge predictions were analyzed retrospectively. All developed models have been published in the QsarDB.org repository according to FAIR principles and can be used without restrictions for exploring, downloading, and making predictions.
Lipid/Clay-Based Solid Dispersion Formulation for Improving the Oral Bioavailability of Curcumin
Authors: Song Jae Geun , Noh Hye-Mi , Lee Sang Hoon , Han Hyo-Kyung
Published: October 2022

This study was conducted to develop a lipid/clay-based solid dispersion (LSD) formulation to enhance the dissolution and oral bioavailability of poorly soluble curcumin. Krill oil and aminoclay were used as a lipid and a stabilizer, respectively, and LSD formulations of curcumin were prepared by an antisolvent precipitation method combined with freeze-drying process. Based on the dissolution profiles, the optimal composition of LSD was determined at the weight ratio of curcumin: krill oil: aminoclay of 1:5:5 in the presence of 0.5% of D-α-tocopherol polyethylene glycol succinate. The structural and morphological characteristics of the LSD formulation were determined using X-ray powder diffraction, differential scanning calorimetry, and scanning electron microscopy. Crystalline curcumin was changed to an amorphous form in the LSD formulation. At the pH of acidic to neutral, the LSD formulation showed almost complete drug dissolution (>90%) within 1 h, while pure curcumin exhibited minimal dissolution of less than 10%. Furthermore, the LSD formulation had significantly improved oral absorption of curcumin in rats, where Cmax and AUC of curcumin were 13- and 23-fold higher for the LSD formulation than for the pure drug. Taken together, these findings suggest that the krill oil-based solid dispersion formulation of curcumin effectively improves the dissolution and oral bioavailability of curcumin.
Modulation of the mTOR Pathway by Curcumin in the Heart of Septic Mice
Authors: Rattis Bruna A. C. , Piva Henrique L. , Duarte Andressa , Gomes Frederico G. F. L. R. , Lellis Janaína R. , Soave Danilo F. , Ramos Simone G. , Tedesco Antonio C. , Celes Mara R. N.
Published: October 2022

mTOR is a signaling pathway involved in cell survival, cell stress response, and protein synthesis that may be a key point in sepsis-induced cardiac dysfunction. Curcumin has been reported in vitro as an mTOR inhibitor compound; however, there are no studies demonstrating this effect in experimental sepsis. Thus, this study aimed to evaluate the action of curcumin on the mTOR pathway in the heart of septic mice. Free curcumin (FC) and nanocurcumin (NC) were used, and samples were obtained at 24 and 120 h after sepsis. Histopathological and ultrastructural analysis showed that treatments with FC and NC reduced cardiac lesions caused by sepsis. Our main results demonstrated that curcumin reduced mTORC1 and Raptor mRNA at 24 and 120 h compared with the septic group; in contrast, mTORC2 mRNA increased at 24 h. Additionally, the total mTOR mRNA expression was reduced at 24 h compared with the septic group. Our results indicate that treatment with curcumin and nanocurcumin promoted a cardioprotective response that could be related to the modulation of the mTOR pathway.
Application of MaxEnt Modeling and HRM Analysis to Support the Conservation and Domestication of Gevuina avellana Mol. in Central Chile
Authors: Moya-Moraga Mario , Pérez-Ruíz César
Published: October 2022

The Chilean hazelnut (Gevuina avellana Mol., Proteaceae) is a native tree of Chile and Argentina of edible fruit-type nut. We applied two approaches to contribute to the development of strategies for mitigation of the effects of climate change and anthropic activities in G. avellana. It corresponds to the first report where both tools are integrated, the MaxEnt model to predict the current and future potential distribution coupled with High-Resolution Melting Analysis (HRM) to assess its genetic diversity and understand how the species would respond to these changes. Two global climate models: CNRM-CM6-1 and MIROC-ES2L for four Shared Socioeconomic Pathways: 126, 245, 370, and 585 (2021–2040; 2061–2080) were evaluated. The annual mean temperature (43.7%) and water steam (23.4%) were the key factors for the distribution current of G. avellana (AUC = 0.953). The future prediction model shows to the year 2040 those habitat range decreases at 50% (AUC = 0.918). The genetic structure was investigated in seven natural populations using eight EST-SSR markers, showing a percentage of polymorphic loci between 18.69 and 55.14% and low genetic differentiation between populations (Fst = 0.052; p < 0.001). According to the discriminant analysis of principal components (DAPC) we identified 10 genetic populations. We conclude that high-priority areas for protection correspond to Los Avellanos and Punta de Águila populations due to their greater genetic diversity and allelic richness.
Spatial and Temporal Variability and Driving Factors of Carbon Dioxide and Nitrous Oxide Fluxes in Alpine Wetland Ecosystems
Authors: Yu Bing , Xu Wenjing , Yan Linlu , Bao Heng , Yu Hongxian
Published: October 2022

Plants regulate greenhouse gas (GHG) fluxes in wetland ecosystems, but the mechanisms of plant removal and plant species that contribute to GHG emissions remain unclear. In this study, the fluxes of carbon dioxide (CO2) and nitrous oxide (N2O) were measured using the static chamber method from an island forest dominated by two different species, namely Betula platyphylla (BP) and Larix gmelinii (LG), in a marsh wetland in the Great Xing’an Mountains. Four sub-plots were established in this study: (1) bare soil after removing vegetation under BP (SBP); (2) bare soil after removing vegetation under LG (SLG); (3) soil with vegetation under BP (VSBP); and (4) soil with vegetation under LG (VSLG). Additionally, the contributions of the dark respiration from plant aerial parts under BP (VBP) and LG (VLG) to GHG fluxes were calculated. We found that the substantial spatial variability of CO2 fluxes ranged from −25.32 ± 15.45 to 187.20 ± 74.76 mg m−2 h−1 during the study period. The CO2 fluxes decreased in the order of SBP > VSLG > VSBP > SLG > VLG > VBP, indicating that vegetation species had a great impact on CO2 emissions. Particularly, the absence of vegetation promoted CO2 emission in both BP and LG. Additionally, CO2 fluxes showed dramatically seasonal variations, with high CO2 fluxes in late spring (May) and summer (June, July, and August), but low fluxes in late summer (August) and early autumn (September). Soil temperatures at 0–20 cm depth were better predictors of CO2 fluxes than deeper soil temperatures. N2O fluxes were varied in different treatments with the highest N2O fluxes in SLG and the lowest N2O fluxes in VBP. Meanwhile, no significant correlation was found between N2O fluxes and air or soil temperatures. Temporally, negative N2O fluxes were observed from June to October, indicating that soil N2O fluxes were reduced and emitted as N2, which was the terminal step of the microbial denitrification process. Most of the study sites were CO2 sources during the warm season and CO2 sinks in the cold season. Thus, soil temperature plays an important role in CO2 fluxes. We also found that the CO2 flux was positively related to pH in a 10 cm soil layer and positively related to moisture content (MC) in a 50 cm soil layer in VSBP and VSLG. However, the CO2 flux was negatively related to pH in a 30 cm soil layer in SBP and SLG. Our findings highlight the effects of vegetation removal on GHG fluxes, and aid in the scientific management of wetland plants.
Effects of Insect-Resistant Maize HGK60 on Community Diversity of Bacteria and Fungi in Rhizosphere Soil
Authors: Wang Yinxiao , Zhang Mingjun , Li Shengyan , Li Pengcheng , Lang Zhihong
Published: October 2022

The influence of biotech crops on microbial communities in rhizosphere soil is an important issue in biosafety assessments. The transgenic maize HGK60 harboring the Bt cry1Ah gene enhanced the resistance to lepidopteran pests, while the ecological risk of HGK60 maize on rhizosphere microorganisms is unclear. In this study, we comprehensively analyzed the diversity and composition of bacterial and fungal communities in the rhizosphere soil around Bt maize HGK60 and the near-isogenic non-Bt maize ZD958 at four growth stages via a high-throughput sequencing technique. The results showed that HGK60 maize unleashed temporary effects on the bacterial and fungal diversity and richness during the study plant’s development, which would be restored after one cycle of plant cultivation due to the application of the same agricultural management. The differences of bacterial and fungal communities were marked by seasonality, while the different growth stage was the important factor as opposed to the cultivar contributing to the shifts in the bacterial and fungal communities’ structure. This study will provide useful information regarding the impact of Bt transgenic maize on the soil microbiome and a theoretical basis for the development of a safety assessment approach for Bt maize in China.
Effects of Graphene Oxide on Plant Growth: A Review
Authors: Yang Yan , Zhang Runxuan , Zhang Xiao , Chen Zezhong , Wang Haiyan , Li Paul Chi Hang
Published: October 2022

Several reports of graphene oxide (GO) promoting plant growth have sparked interest in its potential applications in agroforestry. However, there are still some toxicity studies that have raised concerns about the biosafety of GO. These reports show conflicting results from different perspectives, such as plant physiology, biochemistry, cytology, and molecular biology, regarding the beneficial and detrimental effects of GO on plant growth. Seemingly inconsistent studies make it difficult to effectively apply GO in agroforestry. Therefore, it is crucial to review and analyze the current literature on the impacts of GO on plant growth and its physiological parameters. Here, the biological effects of GO on plant growth are summarized. It is proposed that an appropriate concentration of GO may be conducive to its positive effects, and the particle size of GO should be considered when GO is applied in agricultural applications. This review provides a comprehensive understanding of the effects of GO on plant growth to facilitate its safe and effective use.
The Molecular, Morphological and Genetic Characterization of Glyphosate Resistance in Conyza bonariensis from South Africa
Authors: Okumu Martha N. , Robbertse Petrus J. , Vorster Barend J. , Reinhardt Carl F.
Published: October 2022

Six Conyza bonariensis (L.) Cronquist populations were screened in a pot experiment at the University of Pretoria’s Hatfield experimental farm to evaluate and confirm the degree of glyphosate response. Resistance factors ranged from 2.7- to 24.8-fold compared to the most susceptible biotype. Partial sequencing of the 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) gene found no mutation at the Thr102, Ala103 or Pro106 positions. EPSPS mRNA expression levels in glyphosate-resistant biotypes (Swellendam and Piketberg seed sampling sites) were comparable or lower than those in susceptible biotypes (George and Fauresmith sites). Additionally, the highest expression level was reported in the susceptible Fauresmith biotype. These results indicate that glyphosate resistance in the tested resistant biotypes is not caused by target-site mutations and EPSPS gene amplification. Leaf surface characteristics can influence the spread and subsequent absorption of glyphosate. The study established non-significant results in the amount of leaf wax and insufficient mean separations in cuticle thickness and trichome density data. Therefore, the observed differences in response of biotypes to glyphosate treatment could not be attributed conclusively to differences in the leaf morphological characteristics investigated. Results from the inheritance study were consistent with glyphosate resistance being inherited in an incompletely dominant manner when plants were treated with glyphosate herbicide at 900 g ae ha−1.
Cefazolin Loaded Oxidized Regenerated Cellulose/Polycaprolactone Bilayered Composite for Use as Potential Antibacterial Dural Substitute
Authors: Sanpakitwattana Arunnee , Suvannapruk Waraporn , Chumnanvej Sorayouth , Hemstapat Ruedee , Suwanprateeb Jintamai
Published: October 2022

Oxidized regenerated cellulose/polycaprolactone bilayered composite (ORC/PCL bilayered composite) was investigated for use as an antibacterial dural substitute. Cefazolin at the concentrations of 25, 50, 75 and 100 mg/mL was loaded in the ORC/PCL bilayered composite. Microstructure, density, thickness, tensile properties, cefazolin loading content, cefazolin releasing profile and antibacterial activity against S. aureus were measured. It was seen that the change in concentration of cefazolin loading affected the microstructure of the composite on the rough side, but not on the dense or smooth side. Cefazolin loaded ORC/PCL bilayered composite showed greater densities, but lower thickness, compared to those of drug unloaded composite. Tensile modulus was found to be greater and increased with increasing cefazolin loading, but tensile strength and strain at break were lower compared to the drug unloaded composite. In vitro cefazolin release in artificial cerebrospinal fluid (aCSF) consisted of initial burst release on day 1, followed by a constant small release of cefazolin. The antibacterial activity was observed to last for up to 4 days depending on the cefazolin loading. All these results suggested that ORC/PCL bilayered composite could be modified to serve as an antibiotic carrier for potential use as an antibacterial synthetic dura mater.
Polymeric Nanoparticles for Inhaled Vaccines
Authors: Al-Nemrawi Nusaiba K. , Darweesh Ruba S. , Al-shriem Lubna A. , Al-Qawasmi Farah S. , Emran Sereen O. , Khafajah Areej S. , Abu-Dalo Muna A.
Published: October 2022

Many recent studies focus on the pulmonary delivery of vaccines as it is needle-free, safe, and effective. Inhaled vaccines enhance systemic and mucosal immunization but still faces many limitations that can be resolved using polymeric nanoparticles (PNPs). This review focuses on the use of properties of PNPs, specifically chitosan and PLGA to be used in the delivery of vaccines by inhalation. It also aims to highlight that PNPs have adjuvant properties by themselves that induce cellular and humeral immunogenicity. Further, different factors influence the behavior of PNP in vivo such as size, morphology, and charge are discussed. Finally, some of the primary challenges facing PNPs are reviewed including formulation instability, reproducibility, device-related factors, patient-related factors, and industrial-level scale-up. Herein, the most important variables of PNPs that shall be defined in any PNPs to be used for pulmonary delivery are defined. Further, this study focuses on the most popular polymers used for this purpose.
A Review of Development and Utilization for Edible Fungal Polysaccharides: Extraction, Chemical Characteristics, and Bioactivities
Authors: Sun Yujun , He Huaqi , Wang Qian , Yang Xiaoyan , Jiang Shengjuan , Wang Daobing
Published: October 2022

Edible fungi, commonly known as mushrooms, are precious medicinal and edible homologous gifts from nature to us. Because of their distinctive flavor and exceptional nutritional and medicinal value, they have been a frequent visitor to people’s dining tables and have become a hot star in the healthcare, pharmaceutical, and cosmetics industries. Edible fungal polysaccharides (EFPs) are an essential nutrient for edible fungi to exert bioactivity. They have attracted much attention because of their antioxidant, immunomodulatory, antitumor, hypoglycemic, and hypolipidemic bioactivities. As a result, EFPs have demonstrated outstanding potential over the past few decades in various disciplines, including molecular biology, immunology, biotechnology, and pharmaceutical chemistry. However, the complexity of EFPs and the significant impact of mushroom variety and extraction techniques on their bioactivities prevents a complete investigation of their biological features. Therefore, the authors of this paper thoroughly reviewed the comparison of different extraction methods of EFPs and their advantages and disadvantages. In addition, the molecular weight, monosaccharide composition, and glycosidic bond type and backbone structure of EFPs are described in detail. Moreover, the in vitro and in vivo bioactivities of EFPs extracted by different methods and their potential regulatory mechanisms are summarized. These provide a valuable reference for improving the extraction process of EFPs and their production and development in the pharmaceutical field.
Preparation and Adsorption Properties of Graphene-Modified, Pitch-Based Carbon Foam Composites
Authors: Li Hao , Li Tiehu , Deng Weibin , Kong Siyuan
Published: October 2022

In view of the good adsorption properties of graphene and carbon foam, they were combined to achieve the optimal matching of microstructures. Taking mesophase pitch as a raw material, pitch-based carbon foam was prepared by the self-foaming method. Graphene gel was prepared as the second phase to composite with the carbon foam matrix; graphene-modified, pitch-based carbon foam composites were finally obtained. Graphene gel was dispersed in the rich pore structure of carbon foam to improve its agglomeration and the porosity, and the active sites of the composite were further increased; the adsorption properties and mechanical properties of the composites were also significantly improved. The microstructure and morphology of the composites were studied by SEM, XRD and Raman spectroscopy; the compressive property and porosity were also tested. Methylene blue (MB) solution was used to simulate a dye solution for the adsorption test, and the influence of the composite properties and MB solution on the adsorption property was studied. Results showed that the compressive strength of the composite was 13.5 MPa, increased by 53.41%, and the porosity was 58.14%, increased by 24.15%, when compared to raw carbon foam. When the mass of the adsorbent was 150 mg, the initial concentration of the MB solution was 5 mg/L, and the pH value of the MB solution was 11; the graphene-modified carbon foam composites showed the best adsorption effect, with an adsorption rate of 96.3% and an adsorption capacity of 144.45 mg/g. Compared with the raw carbon foam, the adsorption rate and adsorption capacity of the composites were increased by 158.18% and 93.50%, respectively.
In Vitro and In Vivo Cell-Interactions with Electrospun Poly (Lactic-Co-Glycolic Acid) (PLGA): Morphological and Immune Response Analysis
Authors: Chor Ana , Takiya Christina Maeda , Dias Marcos Lopes , Gonçalves Raquel Pires , Petithory Tatiana , Cypriano Jefferson , de Andrade Leonardo Rodrigues , Farina Marcos , Anselme Karine
Published: October 2022

Random electrospun three-dimensional fiber membranes mimic the extracellular matrix and the interfibrillar spaces promotes the flow of nutrients for cells. Electrospun PLGA membranes were analyzed in vitro and in vivo after being sterilized with gamma radiation and bioactivated with fibronectin or collagen. Madin-Darby Canine Kidney (MDCK) epithelial cells and primary fibroblast-like cells from hamster’s cheek paunch proliferated over time on these membranes, evidencing their good biocompatibility. Cell-free irradiated PLGA membranes implanted on the back of hamsters resulted in a chronic granulomatous inflammatory response, observed after 7, 15, 30 and 90 days. Morphological analysis of implanted PLGA using light microscopy revealed epithelioid cells, Langhans type of multinucleate giant cells (LCs) and multinucleated giant cells (MNGCs) with internalized biomaterial. Lymphocytes increased along time due to undegraded polymer fragments, inducing the accumulation of cells of the phagocytic lineage, and decreased after 90 days post implantation. Myeloperoxidase+ cells increased after 15 days and decreased after 90 days. LCs, MNGCs and capillaries decreased after 90 days. Analysis of implanted PLGA after 7, 15, 30 and 90 days using transmission electron microscope (TEM) showed cells exhibiting internalized PLGA fragments and filopodia surrounding PLGA fragments. Over time, TEM analysis showed less PLGA fragments surrounded by cells without fibrous tissue formation. Accordingly, MNGC constituted a granulomatous reaction around the polymer, which resolves with time, probably preventing a fibrous capsule formation. Finally, this study confirms the biocompatibility of electrospun PLGA membranes and their potential to accelerate the healing process of oral ulcerations in hamsters’ model in association with autologous cells.
Synthesis of Functional Polyesters N-(2,3-epoxypropyl)-4,5,6,7-tetrahydroindole by Anionic Ring-Opening Polymerization
Authors: Markova Marina , Emel’yanov Artem , Tatarinova Inna , Pozdnyakov Alexander
Published: October 2022

The functional polyethers of N-(2,3-epoxypropyl)-4,5,6,7-tetrahydroindole (in up to 61% yield, Mw = 8.7–11.7 kDa) and copolymers with ethylene glycol methylglycidyl ether (Mw = 5.6–14.2 kDa) and ethylene glycol vinylglycidyl ether (Mw = 6.4–12.3 kDa) have been synthesized via anionic ring-opening polymerization in the presence of KOH without solvent. The polymerization involves the opening of the epoxy ring to deliver the linear polyethers bearing free tetrahydroindole rings and oxyethylene or vinyloxy groups in the side chain. The polyethers are soluble in ethanol, benzene, chloroform, dioxane, DMF, and DMSO. The polyethers obtained exhibit the properties of high-resistance organic semiconductors: their electrical conductivity reaches 10−14 S/cm.
PVP/CS/Phyllanthus emblica Nanofiber Membranes for Dry Facial Masks: Manufacturing Process and Evaluations
Authors: Lin Jian-Hong , Shiu Bing-Chiuan , Hsu Po-Wen , Lou Ching-Wen , Lin Jia-Horng
Published: October 2022

In the wake of increasing demands on skin health, we propose simple, natural, and safe dry facial masks that restrict melanin synthesis. Phyllanthus emblica (P. emblica) is made into powders via a low-temperature extraction and freeze-drying process to serve as a natural agent. Next, it is added to mixtures containing Polyvinylpyrrolidone (PVP) and Chitosan (CS), after which the blends are electrospun into PVP/CS/P. emblica nanofiber membrane dry facial masks using the electrospinning technique. The dry facial masks are evaluated using the calibration analysis method, extraction rate test, scanning electron microscopy (SEM), release rate test, tyrosinase inhibition assay, biocompatibility test, and anti-inflammatory capacity test. Test results indicate that when the electrospinning mixture contains 29.0% P. emblica, the nanofibers have a diameter of ≤214.27 ± 74.51 nm and a water contact angle of 77.25 ± 2.21. P. emblica is completely released in twenty minutes, and the tyrosinase inhibition rate reaches 99.53 ± 0.45% and the cell activity ≥82.60 ± 1.30%. Moreover, the anti-inflammatory capacity test results suggest that dry facial masks confine inflammatory factors. PVP/CS/P. emblica nanofiber dry facial masks demonstrate excellent tyrosinase inhibition and are hydrophilic, biocompatible, and inflammation-free. The dry facial masks are a suitable material that is worthwhile exploring and applying to the cosmetic field.
Antibacterial Effect of Triazine in Barrier Membranes with Therapeutic Activity for Guided Bone Regeneration
Authors: de Souza Balbinot Gabriela , Mendes Nobre do Espírito Santo Cassiano , Leitune Vicente Castelo Branco , Visioli Fernanda , Duarte Soares Rosane Michele , Sauro Salvatore , Collares Fabricio Mezzomo
Published: October 2022

Objective: This study aimed to develop polymer-based barrier membranes based on poly(butylene-adipate-co-terephthalate) (PBAT) with the addition of 1,3,5-triacriloilhexahydro-1,3,5-triazine (TAT). Materials and Methods: Polymeric solutions were used to produce membranes with 5 wt% and 10 wt% of TAT by solvent casting. Membranes without the addition of TAT were used as controls. The membranes were chemically characterized by Fourier transform infrared spectroscopy (FTIR) and thermogravimetry (TGA); surface properties were assessed by profilometry and contact angle; the mechanical behavior was evaluated by a tensile test, and the biological properties were assessed by direct–indirect cell viability and antibacterial activity by S. mutans and S. aureus colony-forming units. Results: TAT was detected in the FTIR and TGA analyses and modified the top surface of the membranes, increasing their roughness and wetness in both concentrations compared to the control group (p < 0.05). The addition of TAT, regardless of concentration, reduced the tensile strength and increased membrane stiffness (p < 0.05). The cell viability of 5 wt% TAT and 10 wt% TAT was 86.37% and 82.36%, respectively. All tested concentrations reduced the formation of biofilm on the membranes when compared to the control. Conclusion: The addition of TAT successfully resulted in the antimicrobial ability of PBAT-based barrier membranes, while it maintained acceptable levels of cell viability in membranes with adequate handling and surface properties.
The Electric–Thermal Effect of a Carbon-Fibre-Reinforced Epoxy Composite and Its Corresponding Mechanical Properties
Authors: Zhu Runtian , Wang Guoxian , Lin Yuebin , Long Jinxi , Du Longji , Du Xusheng , Abousnina Rajab , Tafsirojjaman T.
Published: October 2022

In this work, the electric–thermal effect of a carbon-fibre-reinforced epoxy composite (CFRE) panel was studied, as well as the influence of the electric heating treatment on the mechanical properties of the composite. It was observed that the temperature of the composite increased rapidly once the current was loaded, and the equilibrium surface temperature was reached within 2 min. The electric–thermal effect and mechanical properties depended on both the current loading time and the current intensity. At 5A, the flexural modulus and strength of the CFRE increased before decreasing with the current loading time. Under the same treatment time, the flexural strength of the samples treated with 5A was evidently larger than that under the small current, and all the treated samples displayed enhanced flexural strength compared to that of untreated samples. The results depicted that the low-current treatment and short time could improve the interfacial properties between CF/epoxy, along with enhancing the flexural properties of the samples. However, a large amount of the joule heating from the larger current and a more extensive time frame is predicted to cause irreversible defects to the composite, which consequently leads to the reduction in flexural strength of the composite. TGA results indicated decreased thermal stability of the CFRE composite panels after the electric heating treatment was applied.
Greener and Efficient Epoxidation of 1,5-Hexadiene with tert-Butyl Hydroperoxide (TBHP) as an Oxidising Reagent in the Presence of Polybenzimidazole Supported Mo(VI) Catalyst
Authors: Bhuiyan Md Masud Rana , Mohammed Misbahu Ladan , Saha Basudeb
Published: October 2022

Alkene epoxidation with TBHP as an oxidising reagent using heterogeneous Mo(VI) catalyst is an environmentally friendly process since it eliminates acid waste and chlorinated by-products often associated with the conventional industrial method that uses stoichiometric peracid such as peracetic acid and m-chloroperbenzoic acid. Polybenzimidazole supported Mo(VI) complex, i.e., PBI.Mo has been successfully prepared, characterised and assessed for the epoxidation of 1,5-hexadiene in the presence of tert-butyl hydroperoxide (TBHP) as an oxidising reagent. A quadratic polynomial model has been developed, demonstrating the yield of 1,2-epoxy-5-hexene in four independent variables. The effects of different parameters such as reaction temperature, feed mole ratio of 1,5-hexadiene to TBHP, catalyst loading, and reaction time were studied. Response surface methodology (RSM) using Box-Behnken Design (BBD) was employed to study the interaction effect of different variables on the reaction response. This study presents the optimization of 1,5-hexadiene epoxidation in a batch reactor using TBHP as an oxidant and a polymer-supported Mo(VI) catalyst.
Co-Correcting: Combat Noisy Labels in Space Debris Detection
Authors: Li Hui , Niu Zhaodong , Sun Quan , Li Yabo
Published: October 2022

Space debris detection is vital to space missions and space situation awareness. Convolutional neural networks are introduced to detect space debris due to their excellent performance. However, noisy labels, caused by false alarms, exist in space debris detection, and cause ambiguous targets for the training of networks, leading to networks overfitting the noisy labels and losing the ability to detect space debris. To remedy this challenge, we introduce label-noise learning to space debris detection and propose a novel label-noise learning paradigm, termed Co-correcting, to overcome the effects of noisy labels. Co-correcting comprises two identical networks, and the predictions of these networks serve as auxiliary supervised information to mutually correct the noisy labels of their peer networks. In this manner, the effect of noisy labels can be mitigated by the mutual rectification of the two networks. Empirical experiments show that Co-correcting outperforms other state-of-the-art methods of label-noise learning, such as Co-teaching and JoCoR, in space debris detection. Even with a high label noise rate, the network trained via Co-correcting can detect space debris with high detection probability.
Applicability Assessment of Coherent Doppler Wind LiDAR for Monitoring during Dusty Weather at the Northern Edge of the Tibetan Plateau
Authors: Song Meiqi , Wang Yu , Mamtimin Ali , Gao Jiacheng , Aihaiti Ailiyaer , Zhou Chenglong , Yang Fan , Huo Wen , Wen Cong , Wang Bo
Published: October 2022

Wind profile light detection and ranging (LiDAR) is an important tool for observing features within the atmospheric boundary layer. Observations of the wind field and boundary layer height from coherent Doppler wind LiDARs (CDWLs) under sandy and dusty weather conditions were evaluated using observations from two CDWLs and one GTS radio sounding located at the northern edge of the Tibetan plateau from 1 May to 30 August 2021. The results showed that CDWL has good applicability in reproducing wind fields in dust, precipitation, and in clear-sky conditions, and that it is superior to the v wind field for real measurements of the u wind fields. In terms of the planetary boundary layer height (PBLH), the validity of the inversion of PBLH in dusty weather was higher than that under clear-sky conditions. It was found that the PBLH retrieved by the CDWL at 20:00 (BJT) was better than that at 08:00 (BJT). The diurnal variation amplitude of the PBLH before the occurrence of a sandstorm was larger than the diurnal variation amplitude of the PBLH occurring during a sandstorm.
Unblurring ISAR Imaging for Maneuvering Target Based on UFGAN
Authors: Li Wenzhe , Yuan Yanxin , Zhang Yuanpeng , Luo Ying
Published: October 2022

Inverse synthetic aperture radar (ISAR) imaging for maneuvering targets suffers from a Doppler frequency time-varying problem, leading to the ISAR images blurred in the azimuth direction. Given that the traditional imaging methods have poor imaging performance or low efficiency, and the existing deep learning imaging methods cannot effectively reconstruct the deblurred ISAR images retaining rich details and textures, an unblurring ISAR imaging method based on an advanced Transformer structure for maneuvering targets is proposed. We first present a pseudo-measured data generation method based on the DeepLabv3+ network and Diamond-Square algorithm to acquire an ISAR dataset for training with good generalization to measured data. Next, with the locally-enhanced window Transformer block adopted to enhance the ability to capture local context as well as global dependencies, we construct a novel Uformer-based GAN (UFGAN) to restore the deblurred ISAR images with rich details and textures from blurred imaging results. The simulation and measured experiments show that the proposed method can achieve fast and high-quality imaging for maneuvering targets under the condition of a low signal-to-noise ratio (SNR) and sparse aperture.
Study of the Buried Basin C-H, Based on the Multi-Source Remote Sensing Data
Authors: Xu Xiaojian , Hu Teng , Kang Zhizhong , Du Xing , Zhao Lin
Published: October 2022

We use multi-source remote sensing data to identify the details of a mascon south-east of the lunar Copernicus crater. Studies of the topography, gravity, geochronology and mineral are combined to prove that the mascon is a buried peak-ring basin with diameters of about 130 km and 260 km. The underground structure is covered by 890 m thick mare basalts, as determined by analyzing the spectral features of the impact crater, Copernicus H. The determination of the crater size–frequency distribution (CSFD) suggests that the impact that created the C-H basin occurred earlier than 3.9 Ga. Then, a Hawaiian-style eruption in the late Imbrian period formed the Sinus Aestuum-I dark mantling deposit (DMD). Soon, mare basalts covered the basin several times from 3.8 Ga. Finally, the ejecta from the Copernicus impact event at about 800 Ma, and the weathering processes caused the disappearance of the C-H basin rim from the lunar surface.
Analysis of the Three-Dimensional Structure of the Misocyclones Generating Waterspouts Observed by Phased Array Weather Radar: Case Study on 15 May 2017 in Okinawa Prefecture, Japan
Authors: Imai Ryusho , Takahashi Nobuhiro
Published: October 2022

Tornadoes are one of the most severe meteorological phenomena on the earth and their high winds cause serious damage to society. It is well known that vortices (mesocyclone or misocyclone, depending on their scale) in convective clouds contribute to tornadogenesis. High temporal resolution radar observations are necessary to elucidate the mechanism of tornadogenesis because convective clouds change drastically over time. This study focused on waterspouts that occurred on 15 May 2017 near Okinawa, Japan. Using Phased Array Weather Radar (PAWR) data, which provide three-dimensional data with high temporal resolution (every 30 s), radar reflectivity factors and Doppler velocity data were used to detect the three-dimensional structure of vortices. Using PAWR data, vorticities and diameters of these misocyclones were detected every 30 s and their potential vorticities, which are only possible because of the three-dimensional observation by PAWR, were calculated to understand the vortex generation and advection. The structures of two misocyclones (MC1 and MC2) were detected from Doppler velocity patterns. Combined with the radar reflectivity analysis, MC2 can be divided into two misocyclones (MC2a and MC2b). Potential vorticity of MC1 increased with time, probably because an outflow from the strong echo enhanced the lower horizontal shear. Potential vorticities in MC2a and MC2b were conserved in each period, with MC2b being nearly twice as large as MC2a.
Real Micro-Doppler Parameters Extraction of Spinning Targets Based on Rotating Interference Antenna
Authors: Wang Zhihao , Chen Yijun , Yuan Hang , Luo Ying , Zhang Qun
Published: October 2022

Micro-Doppler is a unique characteristic of targets with micro-motions, which can provide significant information for target classification and recognition. However, the monostatic radar has the shortcoming of only obtaining the radial micro-motion characteristics. Although the vortex-electromagnetic-wave-based radar has the potential to obtain real micro-motion parameters, it has a high dependence on the mode number and purity of the orbital angular momentum, which greatly restricts its application in the micro-motion parameter extraction. To overcome the above problems, a new radar configuration based on the rotating interference antenna is proposed in this paper. Through the interference processing of the micro-Doppler curves of the rotating and fixed antenna, the curves containing the real micro-motion information of the target can be obtained. Then the real micro-motion characteristics of the spinning target can be reconstructed by the orthogonal matching pursuit algorithm. The effectiveness and robustness of the proposed method are validated by simulations.
Dithered Depth Imaging for Single-Photon Lidar at Kilometer Distances
Authors: Chang Jiying , Li Jining , Chen Kai , Liu Shuai , Wang Yuye , Zhong Kai , Xu Degang , Yao Jianquan
Published: October 2022

Depth imaging using single-photon lidar (SPL) is crucial for long-range imaging and target recognition. Subtractive-dithered SPL breaks through the range limitation of the coarse timing resolution of the detector. Considering the weak signals at kilometer distances, we present a novel imaging method based on blending subtractive dither with a total variation image restoration algorithm. The spatial correlation is well-considered to obtain more accurate depth profile images with fewer signal photons. Subsequently, we demonstrate the subtractive dither measurement at ranges up to 1.8 km using an array of avalanche photodiodes (APDs) operating in the Geiger mode. Compared with the pixel-wise maximum-likelihood estimation, the proposed method reduces the depth error, which has great promise for high-depth resolution imaging at long-range imaging.
A Novel Water Index Fusing SAR and Optical Imagery (SOWI)
Authors: Tian Bin , Zhang Fangfang , Lang Fengkai , Wang Chen , Wang Chao , Wang Shenglei , Li Junsheng
Published: October 2022

Continuous and accurate acquisitions of surface water distribution are important for water resources evaluation, especially high-precision flood monitoring. During surface water extraction, optical imagery is strongly affected by clouds, while synthetic aperture radar (SAR) imagery is easily influenced by numerous physical factors; thus, the water extraction method based on single-sensor imagery cannot obtain high-precision water range under multiple scenarios. Here, we integrated the radar backscattering coefficient of ground objects into the Normalized Difference Water Index to construct a novel SAR and Optical Imagery Water Index (SOWI), and the water ranges of five study areas were extracted. We compared two previous automatic extraction methods based on single-sensor imagery and evaluated the accuracy of the extraction results. Compared with using optical and SAR imagery alone, the accuracy of all five regions was improved by up to 1–18%. The fusion-derived products resulted in user accuracies ranging 95–99% and Kappa coefficients varying by 85–97%. SOWI was then applied to monitor the 2021 heavy rainfall-induced Henan Province flood disaster, obtaining a time-series change diagram of flood inundation range. Our results verify SOWI’s continuous high-precision monitoring capability to accurately identify waterbodies beneath clouds and algal blooms. By reducing random noise, the defects of SAR are improved and the roughness of water boundaries is overcome. SOWI is suitable for high-precision water extraction in myriad scenarios, and has great potential for use in flood disaster monitoring and water resources statistics.