bluuebunny/crossref_metadata_2025 huggingface dataset:
Yeast Morphology Assessment through Automated Image Analysis during Fermentation
Authors: Guadalupe-Daqui Mario , Chen Mandi , Thompson-Witrick Katherine A. , MacIntosh Andrew J.
Published: March 2021

The kinetics and success of an industrial fermentation are dependent upon the health of the microorganism(s) responsible. Saccharomyces sp. are the most commonly used organisms in food and beverage production; consequently, many metrics of yeast health and stress have been previously correlated with morphological changes to fermentations kinetics. Many researchers and industries use machine vision to count yeast and assess health through dyes and image analysis. This study assessed known physical differences through automated image analysis taken throughout ongoing high stress fermentations at various temperatures (30 °C and 35 °C). Measured parameters included sugar consumption rate, number of yeast cells in suspension, yeast cross-sectional area, and vacuole cross-sectional area. The cell morphological properties were analyzed automatically using ImageJ software and validated using manual assessment. It was found that there were significant changes in cell area and ratio of vacuole to cell area over the fermentation. These changes were temperature dependent. The changes in morphology have implications for rates of cellular reactions and efficiency within industrial fermentation processes. The use of automated image analysis to quantify these parameters is possible using currently available systems and will provide additional tools to enhance our understanding of the fermentation process.
High-Temperature Semi-Dry and Sweet Low Alcohol Wine-Making Using Immobilized Kefir Culture
Authors: Nikolaou Anastasios , Kourkoutas Yiannis
Published: March 2021

Low alcohol wines (≤10.5% vol) represent novel wine products steadily gaining the commercial market interest. Considering the technological advancements of immobilized systems in association with the drastic reduction of industrial operational costs in high-temperature wine-making in regions with tropical climate or hot summer periods, the aim of the present study was to assess the fermentation efficiency of both wet and freeze-dried immobilized kefir culture on natural supports in low alcohol wine production at high temperatures (>30 °C). Immobilized kefir culture was evaluated and compared to free cells in repeated batch fermentations for 3 months, indicating high operational stability, and found suitable for simultaneous alcoholic and malolactic low alcohol wine fermentation at temperatures up to 45 °C. High ethanol productivity [up to 55.3 g/(Ld)] and malic acid conversion rates (up to 71.6%), which could be adopted by the industrial sector, were recorded. Principal Component Analysis (PCA) revealed that the state of the cells rather than the nature of kefir culture affected significantly the content of minor volatiles determined by Head Space Solid-Phase Microextraction (HS-SPME) Gas Chromatography–Mass Spectrometry (GC/MS) analysis. Notably, all new products were of high quality and approved by the sensory panel. The results suggested a high industrial potential of the proposed technology in semi-dry low alcohol wine-making at 37 °C and in developing novel wine products with a sweet (liquoreux) character at 45 °C.
Decentralizing Supply Chain Anti-Counterfeiting and Traceability Systems Using Blockchain Technology
Authors: Yiu Neo C. K.
Published: March 2021

An interesting research problem in the supply chain industry is evaluating and determining the provenance of physical goods—demonstrating the authenticity of luxury goods such as bottled wine. However, many supply chain systems and networks have been built and implemented with centralized system architecture, relying on centralized authorities or any form of intermediary, and leading to issues such as single-point processing, storage and failure, which could be susceptible to malicious modifications to product records or various potential attacks to system components by dishonest participant nodes traversing along the supply chain. Blockchain technology has evolved from merely being a decentralized, distributed and immutable ledger of cryptocurrency transactions to a programmable interactive environment for building decentralized and reliable applications addressing different use-cases and existing problems in the world. In this research, with a chosen research method of proof-by-demonstration, the Decentralized NFC-Enabled Anti-Counterfeiting System (dNAS) is proposed and developed, decentralizing a legacy anti-counterfeiting system of the supply-chain industry using Blockchain technology to facilitate trustworthy data provenance retrieval, verification and management, as well as strengthening the capability of the product’s anti-counterfeiting and traceability qualities in the wine industry, with the capacity to further extend this to the supply chain industry as a whole. The proposed dNAS utilizes a decentralized blockchain network with a consensus protocol compatible with the concept of enterprise blockchain, programmable smart contracts and a distributed file storage system to develop a secure and immutable scientific-data provenance tracking and management platform on which provenance records, providing compelling properties of the data integrity of luxurious goods, are recorded, verified and validated automatically.
Food Consumption Determinants and Barriers for Healthy Eating at the Workplace—A University Setting
Authors: Lima João P. M. , Costa Sofia A. , Brandão Teresa R. S. , Rocha Ada
Published: March 2021

Background: A wide variety of social, cultural and economic factors may influence dietary patterns. This work aims to identify the main determinants of food consumption and barriers for healthy eating at the workplace, in a university setting. Methods: A cross-sectional observational study was conducted with 533 participants. Data were obtained through the application of a self-administered questionnaire that included socio-demographic information, food consumption determinants and the main perceived barriers for healthy eating at the workplace. Results: The respondents identified “price” (22.5%), “meal quality” (20.7%), and “location/distance” (16.5%). For women, the determinant “availability of healthy food options” was more important than for men (p < 0.001). The food consumption determinants at the workplace most referred to by respondents were related to the nutritional value. Smell, taste, appearance and texture, and good value for money, were also considered important for choosing food at the workplace. Respondents referred to work commitments and lack of time as the main barriers for healthy eating at the workplace. Conclusions: Identification of determinants involved in food consumption, and the barriers for healthy eating, may contribute to a better definition of health promotion initiatives at the workplace aiming to improve nutritional intake.
Tetracyclines in Processed Animal Proteins: A Monitoring Study on Their Occurrence and Antimicrobial Activity
Authors: Morello Sara , Pederiva Sabina , Avolio Rosa , Amato Giuseppina , Zoppi Simona , Di Blasio Alessia , Abete Maria Cesarina , Casalone Cristina , Desiato Rosanna , Ru Giuseppe , Marchis Daniela
Published: March 2021

In 2013, the European Union (EU) lifted the feed ban restriction, authorizing the use of non-ruminant (NR) processed animal proteins (PAPs) as ingredient in aquafeed. A further relaxation is soon expected, and NR PAPs will be allowed in next future in poultry and pig feed, avoiding cannibalism. Other potential hazards linked to PAPs as raw material should be evaluated. Antibiotics administered along the lifecycle of animals may leave residue in tissues and bones and still be present in PAPs. This monitoring study aimed to determine tetracyclines (TCLs), known to cumulate in bones, in PAPs and their possible residual antibiotic activity (RAC). A sensitive Liquid Chromatography coupled to Tandem Mass Spectrometry (LC-MS/MS) method for the quantification of TCLs in PAPs was developed and applied to 55 PAPs from EU manufactures. Most PAP samples (n = 40) contained TCLs (concentrations 25.59 ÷ 456.84 µg kg−1). Among samples containing more than 25 µg kg−1 for at least three TCLs, three PAPs were chosen for RAC test before and after TCLs extraction procedure applying an in vitro acidic digestion: in two out of those three samples, RAC was observed after in vitro digestion. TCLs were determined in the digested PAPs (concentrations 26.07 ÷ 64.55 µg kg−1). The detection of TCLs in PAPs should promptly target the risk assessments of this unconsidered way of exposure to antibiotic residues.
Hydrogen Peroxide Effects on Natural-Sourced Polysacchrides: Free Radical Formation/Production, Degradation Process, and Reaction Mechanism—A Critical Synopsis
Authors: Ofoedu Chigozie E. , You Lijun , Osuji Chijioke M. , Iwouno Jude O. , Kabuo Ngozi O. , Ojukwu Moses , Agunwah Ijeoma M. , Chacha James S. , Muobike Onyinye P. , Agunbiade Adedoyin O. , Sardo Giacomo , Bono Gioacchino , Okpala Charles Odilichukwu R. , Korzeniowska Małgorzata
Published: March 2021

Numerous reactive oxygen species (ROS) entities exist, and hydrogen peroxide (H2O2) is very key among them as it is well known to possess a stable but poor reactivity capable of generating free radicals. Considered among reactive atoms, molecules, and compounds with electron-rich sites, free radicals emerging from metabolic reactions during cellular respirations can induce oxidative stress and cause cellular structure damage, resulting in diverse life-threatening diseases when produced in excess. Therefore, an antioxidant is needed to curb the overproduction of free radicals especially in biological systems (in vivo and in vitro). Despite the inherent properties limiting its bioactivities, polysaccharides from natural sources increasingly gain research attention given their position as a functional ingredient. Improving the functionality and bioactivity of polysaccharides have been established through degradation of their molecular integrity. In this critical synopsis; we articulate the effects of H2O2 on the degradation of polysaccharides from natural sources. Specifically, the synopsis focused on free radical formation/production, polysaccharide degradation processes with H2O2, the effects of polysaccharide degradation on the structural characteristics; physicochemical properties; and bioactivities; in addition to the antioxidant capability. The degradation mechanisms involving polysaccharide’s antioxidative property; with some examples and their respective sources are briefly summarised.
Comparing the Rate-All-That-Apply and Rate-All-Statements Question Formats across Five Countries
Authors: Seninde Denis Richard , Chambers Edgar
Published: March 2021

Rate All That Apply (RATA) is a derivative of the popularly used Check-All-That-Apply (CATA) question format. For RATA, consumers select all terms or statements that apply from a given list and then continue to rate those selected based on how much they apply. With Rate All Statements (RATING), a widely used standard format for testing, consumers are asked to rate all terms or statements according to how much they apply. Little is known of how the RATA and RATING question formats compare in terms of aspects such as attribute discrimination and sample differentiation. An online survey using either a RATA or RATING question format was conducted in five countries (Brazil, China, India, Spain, and the USA). Each respondent was randomly assigned one of the two question formats (n = 200 per country per format). Motivations for eating items that belong to five food groups (starch-rich, protein-rich, dairy, fruits and vegetables, and desserts) were assessed. More “apply” responses were found for all eating motivation constructs within RATING data than RATA data. Additionally, the standard indices showed that RATING discriminated more among motivations than RATA. Further, the RATING question format showed better discrimination ability among samples for all motivation constructs than RATA within all five countries. Generally, mean scores for motivations were higher when RATA was used, suggesting that consumers who might choose low numbers in the RATING method decide not to check the term in RATA. More investigation into the validity of RATA and RATING data is needed before use of either question format over the other can be recommended.
Uncertainty Quantification of Random Microbial Growth in a Competitive Environment via Probability Density Functions
Authors: Bevia Vicente José , Burgos Simón Clara , Cortés Juan Carlos , Villanueva Micó Rafael J.
Published: March 2021

The Baranyi–Roberts model describes the dynamics of the volumetric densities of two interacting cell populations. We randomize this model by considering that the initial conditions are random variables whose distributions are determined by using sample data and the principle of maximum entropy. Subsequenly, we obtain the Liouville–Gibbs partial differential equation for the probability density function of the two-dimensional solution stochastic process. Because the exact solution of this equation is unaffordable, we use a finite volume scheme to numerically approximate the aforementioned probability density function. From this key information, we design an optimization procedure in order to determine the best growth rates of the Baranyi–Roberts model, so that the expectation of the numerical solution is as close as possible to the sample data. The results evidence good fitting that allows for performing reliable predictions.
Non-Premixed Filtered Tabulated Chemistry: Filtered Flame Modeling of Diffusion Flames
Authors: Obando Vega Pedro Javier , Coussement Axel , Sadiki Amsini , Parente Alessandro
Published: March 2021

The flame front filtering is a well-known strategy in turbulent premixed combustion. An extension of this approach for the non-premixed combustion context has been proposed by means of directly filtering counterflow diffusion flamelets. Promising results were obtained for the non-premixed filtered tabulated chemistry formalism on 1-D and 2-D unresolved counterflow flame configurations. The present paper demonstrates the soundness of this approach on a 3-D real laminar non-premixed coflow flame. The model results are compared against the direct filtering of the fully resolved laminar diffusion flame showing that the formalism adequately describes the underlying physics. The study reveals the importance of the one-dimensional counterflow flamelet hypothesis, so that the model activation under this condition is ensured by means of a flame sensor. The consistent coupling between the model and the flame sensor adequately retrieves the flame lift-off and satisfactorily predicts the profile extension due to the filtering process.
40 Years of Helicobacter pylori: A Revolution in Biomedical Thought
Authors: Charitos Ioannis Alexandros , D’Agostino Donato , Topi Skender , Bottalico Lucrezia
Published: March 2021

Background: Various microorganisms such as bacteria, virus, and fungi can infect humans and cause not just a simple infection but septic conditions, organ dysfunction, and precancerous conditions or cancer involving various organ systems. After the discovery of the microscope, it was easier to discover and study such microorganisms, as in the case of Helicobacter pylori, a pathogen that was seen in the distant era of the nineteenth century but without being recognized as such. It took 100 years to later discover the pathogenesis and the cancer that this bacterium can cause. Since it was discovered, until today, there has been a continuous search for the understanding of its pathogenetic mechanisms, and the therapeutic approach is continuously updated. Methods: We investigated how diagnosis and therapy were dealt with in the past and how researchers sought to understand, exactly, the pathogenetic biomolecular mechanisms of H. pylori, from the genesis of the infection to the current knowledge, with an analysis of carcinogenic mechanisms in the stomach. We have examined the scientific evolution of the knowledge of the disease over these 40 years in the gastroenterological and pharmacological fields. This was possible through a search in the databases of Medline, the WHO website, the Centers for Disease Control and Prevention (CDC) website, PubMed, and Web of Science to analyze the earlier and the latest data regarding H. pylori. Results: With the scientific discoveries over time, thanks to an increasing number of progressions in scientific research in the analysis of the gastric mucosa, the role of Helicobacter pylori in peptic ulcer, carcinogenesis, and in some forms of gastric lymphoma was revealed. Furthermore, over the years, the biomolecular mechanism involvement in some diseases has also been noted (such as cardiovascular ones), which could affect patients positive for H. pylori. Conclusions: Thanks to scientific and technological advances, the role of the bacterium H. pylori in carcinogenesis has been discovered and demonstrated, and new prospective research is currently attempting to investigate the role of other factors in the stomach and other organs. Cancer from H. pylori infection had a high incidence rate compared to various types of cancer, but in recent years, it is improving thanks to the techniques developed in the detection of the bacterium and the evolution of therapies. Thus, although it has become an increasingly treatable disease, there is still continuous ongoing research in the field of treatment for resistance and pharma compliance. Furthermore, in this field, probiotic therapy is considered a valid adjuvant.
Synthesis and Characterization of Silver–Strontium (Ag-Sr)-Doped Mesoporous Bioactive Glass Nanoparticles
Authors: Bano Shaher , Akhtar Memoona , Yasir Muhammad , Salman Maqbool Muhammad , Niaz Akbar , Wadood Abdul , Ur Rehman Muhammad Atiq
Published: March 2021

Biomedical implants are the need of this era due to the increase in number of accidents and follow-up surgeries. Different types of bone diseases such as osteoarthritis, osteomalacia, bone cancer, etc., are increasing globally. Mesoporous bioactive glass nanoparticles (MBGNs) are used in biomedical devices due to their osteointegration and bioactive properties. In this study, silver (Ag)- and strontium (Sr)-doped mesoporous bioactive glass nanoparticles (Ag-Sr MBGNs) were prepared by a modified Stöber process. In this method, Ag+ and Sr2+ were co-substituted in pure MBGNs to harvest the antibacterial properties of Ag ions, as well as pro-osteogenic potential of Sr2 ions. The effect of the two-ion concentration on morphology, surface charge, composition, antibacterial ability, and in-vitro bioactivity was studied. Scanning electron microscopy (SEM), X-Ray diffraction (XRD), and Fourier transform infrared spectroscopy (FTIR) confirmed the doping of Sr and Ag in MBGNs. SEM and EDX analysis confirmed the spherical morphology and typical composition of MBGNs, respectively. The Ag-Sr MBGNs showed a strong antibacterial effect against Staphylococcus carnosus and Escherichia coli bacteria determined via turbidity and disc diffusion method. Moreover, the synthesized Ag-Sr MBGNs develop apatite-like crystals upon immersion in simulated body fluid (SBF), which suggested that the addition of Sr improved in vitro bioactivity. The Ag-Sr MBGNs synthesized in this study can be used for the preparation of scaffolds or as a filler material in the composite coatings for bone tissue engineering.
Emerging Role of ODC1 in Neurodevelopmental Disorders and Brain Development
Authors: Prokop Jeremy W. , Bupp Caleb P. , Frisch Austin , Bilinovich Stephanie M. , Campbell Daniel B. , Vogt Daniel , Schultz Chad R. , Uhl Katie L. , VanSickle Elizabeth , Rajasekaran Surender , Bachmann André S.
Published: March 2021

Ornithine decarboxylase 1 (ODC1 gene) has been linked through gain-of-function variants to a rare disease featuring developmental delay, alopecia, macrocephaly, and structural brain anomalies. ODC1 has been linked to additional diseases like cancer, with growing evidence for neurological contributions to schizophrenia, mood disorders, anxiety, epilepsy, learning, and suicidal behavior. The evidence of ODC1 connection to neural disorders highlights the need for a systematic analysis of ODC1 genotype-to-phenotype associations. An analysis of variants from ClinVar, Geno2MP, TOPMed, gnomAD, and COSMIC revealed an intellectual disability and seizure connected loss-of-function variant, ODC G84R (rs138359527, NC_000002.12:g.10444500C > T). The missense variant is found in ~1% of South Asian individuals and results in 2.5-fold decrease in enzyme function. Expression quantitative trait loci (eQTLs) reveal multiple functionally annotated, non-coding variants regulating ODC1 that associate with psychiatric/neurological phenotypes. Further dissection of RNA-Seq during fetal brain development and within cerebral organoids showed an association of ODC1 expression with cell proliferation of neural progenitor cells, suggesting gain-of-function variants with neural over-proliferation and loss-of-function variants with neural depletion. The linkage from the expression data of ODC1 in early neural progenitor proliferation to phenotypes of neurodevelopmental delay and to the connection of polyamine metabolites in brain function establish ODC1 as a bona fide neurodevelopmental disorder gene.
Tropical Convection in the Caribbean and Surrounding Region during a Regional, Warming Sea-Surface Temperature Period, 1982–2020
Authors: Glenn Equisha , Smith Thomas M. , Gálvez Jose M. , Davison Michel , Hibbert Keneshia , González Jorge E.
Published: March 2021

Warming sea-surface temperatures (SSTs) have implications for the climate-sensitive Caribbean region, including potential impacts on precipitation. SSTs have been shown to influence deep convection and rainfall, thus understanding the impacts of warming SSTs is important for predicting regional hydrometeorological conditions. This study investigates the long-term annual and seasonal trends in convection using the Galvez-Davison Index (GDI) for tropical convection from 1982–2020. The GDI is used to describe the type and potential for precipitation events characterized by sub-indices that represent heat and moisture availability, cool/warm mid-levels at 500 hPa, and subsidence inversion, which drive the regional Late, Early, and Dry Rainfall Seasons, respectively. Results show that regional SSTs are warming annually and per season, while regionally averaged GDI values are decreasing annually and for the Dry Season. Spatial analyses show the GDI demonstrates higher, statistically significant correlations with precipitation across the region than with sea-surface temperatures, annually and per season. Moreover, the GDI climatology results show that regional convection exhibits a bimodal pattern resembling the characteristic bimodal precipitation pattern experienced in many parts of the Caribbean and surrounding region. However, the drivers of these conditions need further investigation as SSTs continue to rise while the region experiences a drying trend.
A Commentary on the Use of Bivalve Mollusks in Monitoring Metal Pollution Levels
Authors: Yap Chee Kong , Sharifinia Moslem , Cheng Wan Hee , Al-Shami Salman Abdo , Wong Koe Wei , Al-Mutairi Khalid Awadh
Published: March 2021

The objective of this commentary is to promote the use of bivalves as biomonitors, which is a part of the continual efforts of the International Mussel Watch. This commentary is an additional discussion on “Bivalve mollusks in metal pollution studies: From bioaccumulation to biomonitoring” by Zuykov et al., published in Chemosphere 93, 201–208. The present discussion can serve as a platform for further insights to provide new thoughts and novel ideas on how to make better use of bivalves in biomonitoring studies. The certainty of better and more extensive applications of mollusks in environmental monitoring in the future is almost confirmed but more studies are urgently needed. With all the reported studies using bivalves as biomonitors of heavy metal pollution, the effectiveness of using Mussel Watch is beyond any reasonable doubts. The challenge is the development of more accurate methodologies for of heavy metal data interpretation, and the precision of the biomonitoring studies using bivalves as biomonitors, whether in coastal or freshwater ecosystems. Lastly, inclusion of human health risk assessment of heavy metals in commercial bivalves would make the research papers of high public interest.
Control Deficits, Conditioning Factors, and Playing through Pain and Injury among Iranian Professional Soccer Players
Authors: Kabiri Saeed , Choi Jaeyong , Shadmanfaat Seyyedeh Masoomeh (Shamila) , Lee Julak
Published: March 2021

Playing through pain and injury is a common and accepted behavior in the athletic realm. The purpose of this research was to apply Tittle’s control balance theory to explain why athletes engage in playing through pain and injury despite its risky nature. We hypothesized that playing through pain and injury is a form of submission described by Tittle and that it can be predicted by the concept of control deficit. To this end, we collected and used data from a sample of 410 professional soccer players from Guilan province, Iran, and tested several propositions derived from control balance theory. Hierarchical linear regression was used to analyze the data. The study findings demonstrate that players with more control deficits are more likely to play through pain and injury. This relationship is conditioned by self-control, opportunity, motivation, perceived benefits, and provocations. For example, the relationship between control deficit and playing through pain and injury is stronger for those with lower self-control. Our findings support the utility of control balance theory in explaining an act of submission (i.e., playing through pain and injury).
Monitoring Sound and Its Perception during the Lockdown and De-Escalation of COVID-19 Pandemic: A Spanish Study
Authors: Redel-Macías María Dolores , Aparicio-Martinez Pilar , Pinzi Sara , Arezes Pedro , Cubero-Atienza Antonio José
Published: March 2021

The lockdown measures in Spain due to the SARS-CoV-2 or COVID-19 (Coronavirus disease 2019) pandemic from 13 March to 21 June 2020 had extensive social and environmental implications. This study aims to understand how the measures of lockdown have influenced noise levels, as well as people’s perception of sound quality before and after lockdown, including de-escalation. For this purpose, an online survey was carried out. Moreover, the noise linked to the Global Positioning System (GPS) position of each individual respondent was recorded aiming to correlate the noise level with the result of the survey. An average reduction of over 30 dB was observed compared with the sound pressure level before lockdown. Furthermore, it was found that the loudness parameter, together with the overall level, increased as the country started relaxing restrictions. Additionally, results showed that the perception of noise quality changed depending on the phase of de-escalation (p < 0.01), the type of property (p < 0.05), and the outside noise (p < 0.01). Moreover, noise annoyance was determined considering age (p < 0.01), gender (p < 0.05), type of property (p < 0.001), and home refurbishment (p < 0.05). It may be concluded that the most important measure to decrease noise levels is the reduction of traffic noise, through using eco-friendly public transportation or bicycles and limiting nightlife hours.
Population-Level Data on Child Development at School Entry Reflecting Social Determinants of Health: A Narrative Review of Studies Using the Early Development Instrument
Authors: Janus Magdalena , Reid-Westoby Caroline , Raiter Noam , Forer Barry , Guhn Martin
Published: March 2021

Background: The Early Development Instrument (EDI) was developed as a population-level assessment of children’s developmental health at school entry. EDI data collection has created unprecedented opportunities for population-level studies on children’s developmental outcomes. The goal of this narrative review was to synthesize research using the EDI to describe how it contributes to expanding the understanding of the impacts of social determinants on child development and how it applies to special populations. Methods: Select studies published in peer-reviewed scientific journals between 2015 and 2020 and incorporating the social determinants of health perspectives were chosen to highlight the capability of the EDI to monitor children’s developmental health and contribute knowledge in the area of early childhood development. Results: A number of studies have examined the association between several social determinants of health and children’s developmental outcomes, including hard-to-reach and low-frequency populations of children. The EDI has also been used to evaluate programs and interventions in different countries. Conclusions: The ability of the EDI to monitor children’s developmental outcomes in various populations has been consistently demonstrated. The EDI, by virtue of its comprehensive breadth and census-like collection, widens the scope of research relating to early childhood development and its social determinants of health.
The Impact of the COVID-19 Pandemic on Ambient Air Quality in China: A Quasi-Difference-in-Difference Approach
Authors: Zhang Tuo , Tang Maogang
Published: March 2021

The novel coronavirus (COVID-19) pandemic has provided a distinct opportunity to explore the mechanisms by which human activities affect air quality and pollution emissions. We conduct a quasi-difference-in-differences (DID) analysis of the impacts of lockdown measures on air pollution during the first wave of the COVID-19 pandemic in China. Our study covers 367 cities from the beginning of the lockdown on 23 January 2020 until April 22, two weeks after the lockdown in the epicenter was lifted. Static and dynamic analysis of the average treatment effects on the treated is conducted for the air quality index (AQI) and six criteria pollutants. The results indicate that, first, on average, the AQI decreased by about 7%. However, it was still over the threshold set by the World Health Organization. Second, we detect heterogeneous changes in the level of different pollutants, which suggests heterogeneous impacts of the lockdown on human activities: carbon monoxide (CO) had the biggest drop, about 30%, and nitrogen dioxide (NO2) had the second-biggest drop, 20%. In contrast, ozone (O3) increased by 3.74% due to the changes in the NOx/VOCs caused by the decrease in NOx, the decrease of O3 titration, and particulate matter concentration. Third, air pollution levels rebounded immediately after the number of infections dropped, which indicates a swift recovery of human activities. This study provides insights into the implementation of environmental policies in China and other developing countries.
Green Remanufacturer’s Mixed Collection Channel Strategy Considering Enterprise’s Environmental Responsibility and the Fairness Concern in Reverse Green Supply Chain
Authors: Yuan Xigang , Tang Fei , Zhang Dalin , Zhang Xiaoqing
Published: March 2021

In reverse green supply chain, the mixed collection channel strategy of green remanufacturer is analyzed by building a dynamic game model in which we consider that the green remanufacturer undertakes the environmental responsibility and the green collector shows strong fairness concern for the profit. We analyze the impact of the environmental responsibility level of the green remanufacturer, the preference coefficient of the green remanufacturer, the fairness concern coefficient of the green collector, and the coefficient of cross collection price on optimal decision and profit of the green remanufacturer. The result shows that (1) the green remanufacturer can further improve the collection price, so that it makes many more customers participate in the collection activity; (2) the green remanufacturer pays more attention to fulfill the environmental responsibility, which will increase the intensity of collection of the waste green product, and improve the collection price, as the old green product’s remanufacturing cost is lower than the production cost of the new product, and it can improve the green remanufacturer’s profits; (3) the green remanufacturer’s profit in the mixed collection channel is higher than those in online or offline collection channels.
Health Care Services Utilization and Health-Related Quality of Life of Syrian Refugees with Post-Traumatic Stress Symptoms in Germany (the Sanadak Trial)
Authors: Grochtdreis Thomas , Röhr Susanne , Jung Franziska U. , Nagl Michaela , Renner Anna , Kersting Anette , Riedel-Heller Steffi G. , König Hans-Helmut , Dams Judith
Published: March 2021

Refugees who have fled from the ongoing civil war in Syria that arrived in Germany often develop post-traumatic stress symptoms (PTSS). The aim of this study was to determine health care services utilization (HCSU), health care costs and health-related quality of life (HrQoL) of Syrian refugees with mild to moderate PTSS without current treatment in Germany. The study was based on the baseline sample of a randomized controlled trial of a self-help app for Syrian refugees with PTSS (n = 133). HCSU and HrQoL based on the EQ-5D-5L and its visual analogue scale (EQ-VAS) were assessed with standardized interviews. Annual health care costs were calculated using extrapolated four-month HCSU and standardized unit costs. Associations between health care costs, HrQoL and PTSS severity were examined using generalized linear models. Overall, 85.0% of the sample utilized health care services within four months. The mean total annual health care costs were EUR 1920 per person. PTSS severity was not associated with health care costs. The EQ-5D-5L index score and the EQ-VAS score was 0.82 and 73.6, respectively. For Syrian refugees with higher PTSS severity, the EQ-5D-5L index score was lower (−0.17; p < 0.001). The HCSU and the resulting health care costs of Syrian refugees with mild to moderate PTSS without current treatment are low and those with a higher PTSS severity had a lower HrQoL.
Smoking Cessation during the Second Half of Pregnancy Prevents Low Birth Weight among Australian Born Babies in Regional New South Wales
Authors: Ghimire Pramesh Raj , Mooney Julie , Fox Louise , Dubois Lorraine
Published: March 2021

Smoking during pregnancy is a modifiable risk behavior of adverse health outcomes including low birth weight (LBW), and LBW is a key marker of newborns immediate and future health. This study aimed to examine the association between smoking cessation during the second half of pregnancy and LBW among babies born in Southern New South Wales Local Health District (SNSWLHD). Routinely collected perinatal data on singleton live births for the period 2011–2019 in five public hospitals of SNSWLHD were utilized. Multivariate logistic regression models were fitted to examine the association between smoking cessation during the second half of pregnancy and LBW. Analyses showed that mothers who ceased smoking during the second half of pregnancy were 44% less likely to have LBW babies (adjusted odds ratio (aOR) = 0.56; 95% confidence interval (CI): 0.34, 0.94) compared to those who continued smoking throughout pregnancy. Mothers who reported an average daily dose of 1–10 or >10 cigarettes during the second half of pregnancy were significantly more likely to have babies with LBW than those who ceased smoking during the second half of pregnancy. Early identification of smoking behavior and promotion of smoking-cessation intervention for risk populations including pregnant women within the older age bracket (35–49 years) is imperative to reduce LBW.
Compliance with Standard Precautions and Its Relationship with Views on Infection Control and Prevention Policy among Healthcare Workers during COVID-19 Pandemic
Authors: Wong Eliza Lai-Yi , Ho Kin-Fai , Dong Dong , Cheung Annie Wai-Ling , Yau Peter Sen-Yung , Chan Emily Ying-Yang , Yeoh Eng-Kiong , Chien Wai-Tong , Chen Frank Youhua , Poon Simon , Zhang Qingpeng , Wong Samuel Yeung-Shan
Published: March 2021

Background: Standard precautions prevent the spread of infections in healthcare settings. Incompliance with infection control guidelines of healthcare workers (HCWs) may increase their risk of exposure to infectious disease, especially under pandemics. The purpose of this study was to assess the level of compliance with the infection prevention and control practices among HCWs in different healthcare settings and its relationship with their views on workplace infection control measures during the COVID-19 pandemic. Methods: Nurses in Hong Kong were invited to respond to a cross-sectional online survey, in which their views on workplace infection and prevention policy, compliance with standard precautions and self-reported health during pandemics were collected. Results: The respondents were dissatisfied with workplace infection and prevention policy in terms of comprehensiveness (62%), clarity (64%), timeliness (63%), and transparency (60%). For the protective behavior, the respondents did not fully comply with the standard precautions when they were involved in medical care. Their compliance was relatively low when having proper patient handling (54%) and performing invasive procedures (46%). A multivariate analysis model proved that the level of compliance of the standard precautions was positively associated with the satisfaction on infection control and prevention policy among high risk group (0.020; 95% CI: 0.005–0.036), while older respondents had higher level of compliance among the inpatient and outpatient groups (coefficient range: 0.065–0.076). The higher level of compliance was also significantly associated with working in designated team and having chronic condition of the respondents among high-risk and inpatient groups. Conclusions: Standard precautions are the most important elements to reduce cross-transmission among HCWs and patients while the satisfaction on infection control and prevention policy would increase the compliance among the high-risk group. An overall suboptimal compliance and poor views on the infection prevention and control guidelines is a warning signal to healthcare system especially during pandemics.
Spatial Analysis of HIV Infection and Associated Risk Factors in Botswana
Authors: Solomon Malebogo , Furuya-Kanamori Luis , Wangdi Kinley
Published: March 2021

Botswana has the third highest human immunodeficiency virus (HIV) prevalence globally, and the severity of the epidemic within the country varies considerably between the districts. This study aimed to identify clusters of HIV and associated factors among adults in Botswana. Data from the Botswana Acquired Immunodeficiency Syndrome (AIDS) Impact Survey IV (BIAS IV), a nationally representative household-based survey, were used for this study. Multivariable logistic regression and Kulldorf’s scan statistics were used to identify the risk factors and HIV clusters. Socio-demographic characteristics were compared within and outside the clusters. HIV prevalence among the study participants was 25.1% (95% CI 23.3–26.4). HIV infection was significantly higher among the female gender, those older than 24 years and those reporting the use of condoms, while tertiary education had a protective effect. Two significant HIV clusters were identified, one located between Selibe-Phikwe and Francistown and another in the Central Mahalapye district. Clusters had higher levels of unemployment, less people with tertiary education and more people residing in rural areas compared to regions outside the clusters. Our study identified high-risk populations and regions with a high burden of HIV infection in Botswana. This calls for focused innovative and cost-effective HIV interventions on these vulnerable populations and regions to curb the HIV epidemic in Botswana.
In Vitro Anticancer Potential of Jasione montana and Its Main Components against Human Amelanotic Melanoma Cells
Authors: Juszczak Aleksandra Maria , Czarnomysy Robert , Strawa Jakub Władysław , Zovko Končić Marijana , Bielawski Krzysztof , Tomczyk Michał
Published: March 2021

Jasione montana L. (Campanulaceae) is used in traditional Belarusian herbal medicine for sleep disorders in children, but the chemical composition and biological activity have not been investigated. In this study, the activities of J. montana extracts, their fractions and main compounds were evaluated in amelanotic melanoma C32 (CRL-1585) cells and normal fibroblasts (PCS-201-012). The extracts and fractions were analyzed using liquid chromatography–photodiode array detection–electrospray ionization–mass spectrometry (LC–PDA–ESI–MS/TOF) to characterize 25 compounds. Further, three major and known constituents, luteolin (22) and its derivatives such as 7-O-glucoside (12) and 7-O-sambubioside (9) were isolated and identified. The cytotoxic activities against fibroblasts and the amelanotic melanoma cell line were determined using the fixable viability stain (FVS) assay. The influence of diethyl ether (Et2O) fraction (JM4) and 22 on apoptosis induction was investigated using an annexin V binding assay. The obtained results showed significant cytotoxicity of JM4 and 22 with IC50 values of 119.7 ± 3.2 and 95.1 ± 7.2 μg/mL, respectively. The proapoptotic potential after 22 treatment in the C32 human amelanotic melanoma cell line was comparable to that of vinblastine sulfate (VLB), detecting 29.2 ± 3.0% apoptotic cells. Moreover, 22 displayed less necrotic potential against melanoma cells than VLB. In addition, the influences of JM4 and 22 on the dysfunction of the mitochondrial membrane potential (MMP), cell cycle and activity of caspases 3, 8, 9, and 10 were established. The effects of JM4 on MMP change (74.5 ± 3.0% of the cells showed a reduced MMP) corresponded to the results obtained from the annexin V binding assay and activation of caspase-9. JM4 and 22 displayed a significant impact on caspase-9 (40.9 ± 2.4% of the cells contained active caspase-9 after JM4 treatment and 16.6 ± 0.8% after incubation with 22) and the intrinsic (mitochondrial) apoptotic pathway. Moreover, studies have shown that JM4 and 22 affect the activation of external apoptosis pathways by inducing the caspase-8 and caspase-10 cascades. Thus, activation of caspase-3 and DNA damage via external and internal apoptotic pathways were observed after treatment with JM4 and 22. The obtained results suggest that J. montana extracts could be developed as new topical preparations with potential anticancer properties due to their promising cytotoxic and proapoptotic potential.
Enzymatic and Chemical Cross-Linking of Bacterial Cellulose/Fish Collagen Composites—A Comparative Study
Authors: Sommer Agata , Dederko-Kantowicz Paulina , Staroszczyk Hanna , Sommer Sławomir , Michalec Marek
Published: March 2021

This article compares the properties of bacterial cellulose/fish collagen composites (BC/Col) after enzymatic and chemical cross-linking. In our methodology, two transglutaminases are used for enzymatic cross-linking—one recommended for the meat and the other proposed for the fish industry—and pre-oxidated BC (oxBC) is used for chemical cross-linking. The structure of the obtained composites is characterized by scanning electron microscopy, thermogravimetric analysis, X-ray diffraction, and Fourier transform infrared spectroscopy, and their functional properties by mechanical and water barrier tests. While polymer chains in uncross-linked BC/Col are intertwined by H-bonds, new covalent bonds in enzymatically cross-linked ones are formed—resulting in increased thermal stability and crystallinity of the material. The C2–C3 bonds cleavage in D-glucose units, due to BC oxidation, cause secondary alcohol groups to vanish in favor of the carbonyl groups’ formation, thus reducing the number of H-bonded OHs. Thermal stability and crystallinity of oxBC/Col remain lower than those of BC/Col. The BC/Col formation did not affect tensile strength and water vapor permeability of BC, but enzymatic cross-linking with TGGS improved them significantly.
Knockout of Auxin Response Factor SlARF4 Improves Tomato Resistance to Water Deficit
Authors: Chen Mengyi , Zhu Xiaoyang , Liu Xiaojuan , Wu Caiyu , Yu Canye , Hu Guojian , Chen Lin , Chen Riyuan , Bouzayen Mondher , Zouine Mohammed , Hao Yanwei
Published: March 2021

Auxin response factors (ARFs) play important roles in various plant physiological processes; however, knowledge of the exact role of ARFs in plant responses to water deficit is limited. In this study, SlARF4, a member of the ARF family, was functionally characterized under water deficit. Real-time fluorescence quantitative polymerase chain reaction (PCR) and β-glucuronidase (GUS) staining showed that water deficit and abscisic acid (ABA) treatment reduced the expression of SlARF4. SlARF4 was expressed in the vascular bundles and guard cells of tomato stomata. Loss of function of SlARF4 (arf4) by using Clustered Regularly Interspaced Short Palindromic Repeats/Cas 9 (CRISPR/Cas 9) technology enhanced plant resistance to water stress and rehydration ability. The arf4 mutant plants exhibited curly leaves and a thick stem. Malondialdehyde content was significantly lower in arf4 mutants than in wildtype plants under water stress; furthermore, arf4 mutants showed higher content of antioxidant substances, superoxide dismutase, actual photochemical efficiency of photosystem II (PSII), and catalase activities. Stomatal and vascular bundle morphology was changed in arf4 mutants. We identified 628 differentially expressed genes specifically expressed under water deficit in arf4 mutants; six of these genes, including ABA signaling pathway-related genes, were differentially expressed between the wildtype and arf4 mutants under water deficit and unlimited water supply. Auxin responsive element (AuxRE) elements were found in these genes’ promoters indicating that SlARF4 participates in ABA signaling pathways by regulating the expression of SlABI5/ABF and SCL3, thereby influencing stomatal morphology and vascular bundle development and ultimately improving plant resistance to water deficit.
Potential of Nutraceutical Supplementation in the Modulation of White and Brown Fat Tissues in Obesity-Associated Disorders: Role of Inflammatory Signalling
Authors: Scarano Federica , Gliozzi Micaela , Zito Maria Caterina , Guarnieri Lorenza , Carresi Cristina , Macrì Roberta , Nucera Saverio , Scicchitano Miriam , Bosco Francesca , Ruga Stefano , Coppoletta Anna Rita , Mollace Rocco , Maiuolo Jessica , Bava Irene , Cardamone Antonio , Ragusa Monica , Palma Ernesto , Musolino Vincenzo , Mollace Vincenzo
Published: March 2021

The high incidence of obesity is associated with an increasing risk of several chronic diseases such as cardiovascular disease, type 2 diabetes and non-alcoholic fatty liver disease (NAFLD). Sustained obesity is characterized by a chronic and unsolved inflammation of adipose tissue, which leads to a greater expression of proinflammatory adipokines, excessive lipid storage and adipogenesis. The purpose of this review is to clarify how inflammatory mediators act during adipose tissue dysfunction in the development of insulin resistance and all obesity-associated diseases. In particular, we focused our attention on the role of inflammatory signaling in brown adipose tissue (BAT) thermogenic activity and the browning of white adipose tissue (WAT), which represent a relevant component of adipose alterations during obesity. Furthermore, we reported the most recent evidence in the literature on nutraceutical supplementation in the management of the adipose inflammatory state, and in particular on their potential effect on common inflammatory mediators and pathways, responsible for WAT and BAT dysfunction. Although further research is needed to demonstrate that targeting pro-inflammatory mediators improves adipose tissue dysfunction and activates thermogenesis in BAT and WAT browning during obesity, polyphenols supplementation could represent an innovative therapeutic strategy to prevent progression of obesity and obesity-related metabolic diseases.