bluuebunny/crossref_metadata_2025 huggingface dataset:
Microencapsulation of Phenolic Compounds Extracted from Okra (Abelmoschus esculentus L.) Leaves, Fruits and Seeds
Authors: Guebebia Salma , Gharsallaoui Adem , Dumas Emilie , Baghi Fatemeh , Zourgui Lazhar , Romdhane Mehrez , Agusti Géraldine , Ghnimi Sami
Published: November 2023

Several plants rich in phenolic compounds have many uses in the food and pharmaceutical fields. However, after extraction, these active biomolecules are susceptible to degradation. Microencapsulation is a possible solution to prevent this rapid degradation. In this study, phenolic compounds from the okra Abelmoschus esculentus L’s leaves, fruits and seeds were extracted using microwave-assisted extraction and then microencapsulated via the spray drying technique using maltodextrin combined with pectin (in a ratio of 10:1) as an encapsulation material. The total phenolic content, DPPH scavenging and antimicrobial activities of okra extracts and encapsulated samples were evaluated to verify the encapsulation efficiency. Particle size distribution determination and scanning electron microscopy of the microcapsules were also carried-out. The ethanolic leaf extract showed higher significant levels of total phenolic compounds (162.46 ± 4.48 mg GAE/g DW), and anti-oxidant (75.65%) and antibacterial activities compared to those of other aqueous and ethanolic extracts from fruits and seeds. Furthermore, the spray-dried ethanolic leaf extract had the highest total phenolic content. However, the encapsulated ethanolic fruit extract had the highest percentage of DPPH scavenging activity (30.36% ± 1.49). In addition, antibacterial activity measurements showed that the addition of ethanolic and aqueous seed microcapsules provided a significant zone of inhibition against the bacterium Brochotrix thermosphacta (38 mm and 30 mm, respectively). Okra aqueous leaf microcapsules showed the smallest Sauter mean diameter values (7.98 ± 0.12 µm). These data are applicable for expanding the use of okra leaves, fruits and seeds as food additives and/or preservatives in the food industry.
Stochastic Subspace Identification-Based Automated Operational Modal Analysis Considering Modal Uncertainty
Authors: Cho Keunhee , Cho Jeong-Rae
Published: November 2023

An automated operational modal analysis (AOMA) method that considers the uncertainty in modal parameters is presented and data acquired from actual bridges are used to validate it. The proposed method processes stepwise, from SSI to pre-cleaning, clustering and the removal of outliers. The stochastic subspace identification (SSI) step also calculates the uncertainty of the modal parameters. In this step, the MAC (modal assurance criterion) index and its variability are additionally calculated by exploiting the alteration of the mode shapes. The pre-cleaning stage sorts out the spurious modes by means of the frequency, the coefficient of variation related to the frequency and the damping ratio, as well as the MAC index and its standard deviation. Under the assumption of normal distributions for the frequency and the MAC index, the clustering stage constructs clusters of identical modes with reference to the uncertainty of each mode. The outliers that may be contained in each of these clusters are then removed based upon the frequency, the MAC index and the damping ratio. Values for the parameters that make the proposed method applicable are suggested and are applied unilaterally to three instrumented bridges of different types. The results show that the proposed AOMA method provides accurate mode identification regardless of the bridge type.
Advancing Complexity Research in Earth Sciences and Geography
Authors: Gao Jianbo
Published: November 2023

Many complex phenomena in earth sciences and geography, including nonlinear fluid motions in the atmosphere, oceans, rivers, and lakes, coastal morpho dynamics, volcanic and seismic activities, the spatiotemporal dynamics of species, human movement trajectory, and city transportation dynamics, among many others, have played significant roles in the creation and development of complexity science, particularly chaos theory and fractal geometry [...]
Effect of Inter-Vehicle Suspension on Variable Speed Curve Running of Train under Crosswinds
Authors: Jia Xiaochen , Jin Afang , Chen Leixin , Yang Dexin
Published: November 2023

High-speed trains operating in windy areas may accelerate and decelerate frequently to maintain safe travel, especially when passing curves. During acceleration and deceleration, the role of inter-vehicle suspension (IVS) cannot be ignored. The present study aims to evaluate the effect of IVS on the variable speed curve running of trains under crosswinds. To achieve this purpose, a multibody model of a China Railways High-speed 2 (CRH2) high-speed train considering the IVS is established. By inputting the crosswind loads and traction or braking forces to the model and setting curved tracks with different radii and the unloading factor set as safety criterion, the safe running speeds of the train under different crosswind speeds and different track radii were obtained. The difference in the vehicle dynamics considering the IVS and the fixed connection under traction and braking conditions is analyzed. The radius of the curve track significantly affects the safety characteristics of a train under crosswinds, but its impact diminishes for radii greater than 7000 m. The lateral acceleration, movement angle, unloading factor, and derailment coefficient in both acceleration and deceleration cases of car bodies are affected by the IVS. As a consequence, the IVS will lead to lower safe speeds than fixed connections, but it will also convey more realistic and credible train dynamics.
Black PEO Coatings on Titanium and Titanium Alloys Produced at Low Current Densities
Authors: Kostelac Lorena , Pezzato Luca , Colusso Elena , Natile Marta Maria , Brunelli Katya , Dabalà Manuele
Published: November 2023

Black coatings were successfully formed on Grade 2 (G2) and Grade 5 (G5) titanium alloy by means of a direct-current Plasma Electrolytic Oxidation (PEO) process at a very low current density of 0.05 A/cm2. The impact of two different treatment times (30 min and 60 min) was examined. The electrolyte for the PEO process was a phosphate base solution Na5P3O10 containing FeSO4 and (NH4)6Mo7O24 as coloring additives. PEO-coated samples were subjected to optical, morphological, structural, chemical, and electrochemical characterization. XRD, EDS, and XPS data analyses revealed that anion MoO42− and metal cation Fe3+ were successfully incorporated into the coatings. The results demonstrated that PEO-coated samples prepared after 60 min exhibit a stronger black color than those created after 30 min, with an absorptance maximum of 0.86. Furthermore, all prepared PEO coatings improve the corrosion resistance of bare titanium. Among them, the 60-minute PEO coatings on both alloys were the ones with the best corrosion properties.
The Theory of Value and the Understanding of Authenticity: Keys to Intervening in Heritage Spaces: Results in the Case of San Telmo Palace (Seville 17th–21st Century, Spain)
Authors: Gómez-Villa José-Luis , García-Casasola Marta , Mosquera-Adell Eduardo
Published: November 2023

The cultural theory of heritage assets that was consolidated throughout the 20th century, and is still in force today, lies in the modulation of the concept of authenticity. It is a nomadic, controversial concept that has adapted to the spirit of the times. This review of the concept, drawing on international reference texts and charters in the field of heritage, allows us to specify a working process that facilitates its consideration in characterisation processes. To do so, case studies of significant cultural assets in terms of scale and complexity will be presented as resources. Through the implementation of the Theory of Value, the keys will be provided: criteria and methodology for intervening in heritage. This journey will be reflected more slowly in the study of the Palace of San Telmo (Seville, 17th–21st century). This is a building with a controversial material history, in which a series of attributions of value have been transmitted that correlate both with the theory of the conservation of cultural assets and with social appreciation and the criteria. This is the way to materially undertake a reflection on the authenticity of this heritage. This study provides the keys to intervening, conserving the values identified, and recognising authenticity as a reality that is constructed through the process and through the passage of time. A methodology that allows us to consider the capacity to continue and replace heritage as a project strategy in which what is yet to come can take place.
Analysis of Air Pollutants and Their Potential Sources in Eastern Xinjiang, Northwestern Inland China, from 2018 to 2022
Authors: Zhang Yuanyuan , Xu Hui , Zhang Yunhui , Luo Jie , Chen Fuyao , Cao Bo , Xie Mingjie
Published: November 2023

Air pollution in the developed regions of eastern China has been intensively investigated in the past decade. However, there is a relative dearth of air pollution studies on the northwest of inland China (e.g., Xinjiang). In this work, hourly measurement data of six criteria air pollutants (PM2.5, PM10, CO, NO2, O3, and SO2) for the past five years (2018–2022) from Hami and Turpan cities of eastern Xinjiang were analyzed to reveal air pollution characteristics and the distribution of potential sources. Hami and Turpan had the highest AQI values in winter due to increased coal combustion for domestic heating and unfavorable meteorological conditions. The slight elevations of AQI values in spring were caused by frequent dust storms. PM10 was the most frequent main pollutant in both Hami (63.1%) and Turpan (74.1%), followed by PM2.5 and O3. Except for O3, PM2.5, PM10, SO2, NO2, and CO exhibited a generally decreasing pattern in annual average values. But the annual average concentrations of PM10 in Hami (83.5 μg·m−3) and Turpan (139 μg·m−3) in 2022 were still higher than those in eastern China. Diurnal and monthly variations of the six criteria pollutants were influenced by a combination of emission sources and meteorological conditions. The air masses in eastern Xinjiang mainly originated from the west and north and were affected by both inter-regional and intra-regional transport. Analysis of the distribution of potential sources showed that local emissions strongly impacted particulate matter pollution in winter, while regional transport played a dominant role in other seasons. O3 showed a broad distribution of potential sources across all four seasons. Considering that the trend that O3 pollution increased year by year, eastern Xinjiang might face a similar pollution situation as eastern China, i.e., the combined pollution of particulate matter and O3.
An Optimized Framework for Precipitable Water Vapor Mapping Using TS-InSAR and GNSS
Authors: Guo Qiuying , Yu Miao , Li Dewei , Huang Shoukai , Xue Xuelong , Sun Yingjun , Zhou Chenghu
Published: November 2023

Observations of precipitable water vapor (PWV) in the atmosphere play a crucial role in weather forecasting and global climate change research. Spaceborne Interferometric Synthetic Aperture Radar (InSAR), as a widely used modern geodetic technique, offers several advantages to the mapping of PWV, including all-weather capability, high accuracy, high resolution, and spatial continuity. In the process of PWV retrieval by using InSAR, accurately extracting the tropospheric wet delay phase and obtaining a high-precision tropospheric water vapor conversion factor are critical steps. Furthermore, the observations of InSAR are spatio-temporal differential results and the conversion of differential PWV (InSAR ΔPWV) into non-difference PWV (InSAR PWV) is a difficulty. In this study, the city of Jinan, Shandong Province, China is selected as the experimental area, and Sentinel-1A data in 2020 is used for mapping InSAR ΔPWV. The method of small baseline subset of interferometry (SBAS) is adopted in the data processing for improving the coherence of the interferograms. We extract the atmosphere phase delay from the interferograms by using SRTM-DEM and POD data. In order to evaluate the calculation of hydrostatic delay by using the ERA5 data, we compared it with the hydrostatic delay calculated by the Saastamoinen model. To obtain a more accurate water vapor conversion factor, the value of the weighted average temperature Tm was calculated by the path integral of the ERA5. In addition, GNSS PWV is used to calibrate InSAR PWV. This study demonstrates a robust consistency between InSAR PWV and GNSS PWV, with a correlation coefficient of 0.96 and a root-mean-square error (RMSE) of 1.62 mm. In conclusion, our method ensures the reliability of mapping PWV by using Sentinel-1A interferograms and GNSS observations.
Complex Generalized Representation of Gamma Function Leading to the Distributional Solution of a Singular Fractional Integral Equation
Authors: Tassaddiq Asifa , Srivastava Rekha , Kasmani Ruhaila Md , Alharbi Rabab
Published: November 2023

Firstly, a basic question to find the Laplace transform using the classical representation of gamma function makes no sense because the singularity at the origin nurtures so rapidly that Γze−sz cannot be integrated over positive real numbers. Secondly, Dirac delta function is a linear functional under which every function f is mapped to f(0). This article combines both functions to solve the problems that have remained unsolved for many years. For instance, it has been demonstrated that the power law feature is ubiquitous in theory but challenging to observe in practice. Since the fractional derivatives of the delta function are proportional to the power law, we express the gamma function as a complex series of fractional derivatives of the delta function. Therefore, a unified approach is used to obtain a large class of ordinary, fractional derivatives and integral transforms. All kinds of q-derivatives of these transforms are also computed. The most general form of the fractional kinetic integrodifferential equation available in the literature is solved using this particular representation. We extend the models that were valid only for a class of locally integrable functions to a class of singular (generalized) functions. Furthermore, we solve a singular fractional integral equation whose coefficients have infinite number of singularities, being the poles of gamma function. It is interesting to note that new solutions were obtained using generalized functions with complex coefficients.
On Hybrid Hyper k-Pell, k-Pell–Lucas, and Modified k-Pell Numbers
Authors: Spreafico Elen Viviani Pereira , Catarino Paula , Vasco Paulo
Published: November 2023

Many different number systems have been the topic of research. One of the recently studied number systems is that of hybrid numbers, which are generalizations of other number systems. In this work, we introduce and study the hybrid hyper k-Pell, hybrid hyper k-Pell–Lucas, and hybrid hyper Modified k-Pell numbers. In order to study these new sequences, we established new properties, generating functions, and the Binet formula of the hyper k-Pell, hyper k-Pell–Lucas, and hyper Modified k-Pell sequences. Thus, we present some algebraic properties, recurrence relations, generating functions, the Binet formulas, and some identities for the hybrid hyper k-Pell, hybrid hyper k-Pell–Lucas, and hybrid hyper Modified k-Pell numbers.
Production of Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from CO2 via pH-Stat Jar Cultivation of an Engineered Hydrogen-Oxidizing Bacterium Cupriavidus necator
Authors: Tanaka Kenji , Orita Izumi , Fukui Toshiaki
Published: November 2023

The copolyester of 3-hydroxybutyrate (3HB) and 3-hydoxyhexanoate (3HHx), PHBHHx, is a biodegradable plastic characterized by high flexibility, softness, a wide process window, and marine biodegradability. PHBHHx is usually produced from structurally related carbon sources, such as vegetable oils or fatty acids, but not from inexpensive carbon sources such as sugars. In previous studies, we demonstrated that engineered strains of a hydrogen-oxidizing bacterium, Cupriavidus necator, synthesized PHBHHx with a high cellular content not only from sugars but also from CO2 as the sole carbon source in the flask culture. In this study, the highly efficient production of PHBHHx from CO2 was investigated via pH-stat jar cultivation of recombinant C. necator strains while feeding the substrate gas mixture (H2/O2/CO2 = 80:10:10 v/v%) to a complete mineral medium in a recycled-gas, closed-circuit culture system. As a result, the dry cell mass and PHBHHx concentration with the strain MF01/pBPP-ccrMeJAc-emd reached up to 59.62 ± 3.18 g·L−1 and 49.31 ± 3.14 g·L−1, respectively, after 216 h of jar cultivation with limited addition of ammonia and phosphate solutions. The 3HHx composition was close to 10 mol%, which is suitable for practical applications. It is expected that the autotrophic cultivation of the recombinant C. necator can be feasible for the mass production of PHBHHx from CO2.
Numerical Simulation of the Effect of Different Footwear Midsole Structures on Plantar Pressure Distribution and Bone Stress in Obese and Healthy Children
Authors: Zhou Qixuan , Niu Wenxin , Yick Kit-Lun , Gu Bingfei , Sun Yue
Published: November 2023

The foot, as the foundation of the human body, bears the vast majority of the body’s weight. Obese children bear more weight than healthy children in the process of walking and running. This study compared three footwear midsole structures (solid, lattice, and chiral) based on plantar pressure distribution and bone stress in obese and healthy children through numerical simulation. The preparation for the study included obtaining a thin-slice CT scan of a healthy 9-year-old boy’s right foot, and this study distinguished between a healthy and an obese child by applying external loadings of 25 kg and 50 kg in the finite element models. The simulation results showed that the plantar pressure was mainly concentrated in the forefoot and heel due to the distribution of gravity (first metatarsal, fourth metatarsal, and heel bone, corresponding to plantar regions M1, M4, and HM and HL) on the foot in normal standing. Compared with the lattice and solid EVA structures, in both healthy and obese children, the percentage reduction in plantar pressure due to the chiral structure in the areas M1, M4, HM, and HL was the largest with values of 38.69%, 34.25%, 64.24%, and 54.03% for an obese child and 33.99%, 28.25%, 56.08%, and 56.96% for a healthy child. On the other hand, higher pressures (15.19 kPa for an obese child and 5.42 kPa for a healthy child) were observed in the MF area when using the chiral structure than when using the other two structures, which means that this structure can transfer an amount of pressure from the heel to the arch, resulting in a release in the pressure at the heel region and providing support at the arch. In addition, the study found that the chiral structure was not highly sensitive to the external application of body weight. This indicates that the chiral structure is more stable than the other two structures and is minimally affected by changes in external conditions. The findings in this research lay the groundwork for clinical prevention and intervention in foot disorders in obese children and provide new research ideas for shoe midsole manufacturers.
Biomechanical Comparisons of Different Reconstructive Techniques for Scapholunate Dissociation: A Cadaveric Study
Authors: Park Il-Jung , Chae Seungbum , Kwak Dai-Soon , Kim Yoon-Vin , Ha Seunghun , Lim Dohyung
Published: November 2023

There are many techniques for the treatment of chronic scapholunate dissociation. The three-ligament tenodesis (3LT) is used most widely, but reconstruction of the dorsal ligament alone may not provide sufficient stability. The Mark–Henry technique (MHT) compensates for the insufficient stability of 3LT by additional reconstruction of the volar ligament, but the procedure is complex. The SwiveLock technique (SWT), a recently introduced method, provides stability by using autologous tendons with synthetic tapes, but lacks long-term clinical results. To perform biomechanical comparisons of different reconstructive techniques for scapholunate dissociation using a controlled laboratory cadaveric model. Eleven fresh-frozen upper-extremity cadaveric specimens were prepared. The scapholunate distance, scaphoid rotation, and lunate rotation of the specimens were measured during continuous flexion–extension and ulnar–radial deviation movements. The data were collected using a wrist simulator with a linear guide rail system (tendon load/motion-controlled system) and a motion capture system. Results were compared in five conditions: (1) intact, (2) scapholunate dissociation, (3) SWT, (4) 3LT, and (5) MHT. Paired t-test was employed to compare the biomechanical characteristics of intact wrists to those of scapholunate dissociated wrists, and to those of wrists after each of the three reconstruction methods. SWT and MHT were effective solutions for reducing the widening in scapholunate distance. According to the radioscaphoid angle, all three reconstruction techniques were effective in addressing the flexion deformity of the scaphoid. According to the radiolunate angle, only SWT was effective in addressing the extension deformity of the lunate. In terms of scapholunate angle, only the results after SWT did not differ from those of the intact wrist. The SWT technique most effectively improved distraction intensity and rotational strength for the treatment of scapholunate dissociation. Taking into account the technical complexity of 3LT and MHT, SWT may be a more efficient technique to reduce operating time and minimize complications due to multiple incisions, transosseous tunnels, and complicated shuttling.
Evaluation of Bone Repair Using a New Biphasic Synthetic Bioceramic (Plenum® Osshp) in Critical Calvaria Defect in Rats
Authors: Frigério Paula Buzo , Quirino Lilian Caldas , Gabrielli Marisa Aparecida Cabrini , Carvalho Pedro Henrique de Azambuja , Garcia Júnior Idelmo Rangel , Pereira-Filho Valfrido Antonio
Published: November 2023

(1) Background: Biphasic bioceramics are synthetic bone substitutes that provide greater safety and better predictability in guided bone regeneration. This study aimed to evaluate the bone repair process using a new biphasic bioceramic of synthetic origin (Plenum® Osshp—70HA: 30β-TCP) in critical calvarial defects. (2) Methods: seventy-four defects were created in rat calvaria and divided into two groups—Plenum® Osshp (PO), right side, and Straumann® BoneCeramic™ (BC), left side. Euthanasia was performed at 7, 15, 30, and 60 days after surgery. (3) Results: Lower gene expression was observed for runt-related transcription factor 2 (RUNX2) and vascular endothelial growth factor (VEGF) and higher expression for Integrin Binding Sialoprotein (IBSP). The results correlated with moderate immunolabeling for osteocalcin (OCN) and slight immunolabeling for osteopontin (OPN) in the PO group. Histometry showed a greater amount of biomaterial remaining in the PO group at 60 days. The microtomographic analysis showed a lower density of bone connectivity and a greater thickness of the trabeculae for the remnants of the PO group. (4) Conclusions: the Plenum® Osshp showed no differences compared to BoneCeramic™ and is therefore considered an effective option as a synthetic bone substitute in bone regeneration.
The Emerging Role of Induced Pluripotent Stem Cells as Adoptive Cellular Immunotherapeutics
Authors: Mehra Vedika , Chhetri Jyoti Bikram , Ali Samira , Roddie Claire
Published: November 2023

Adoptive cell therapy (ACT) has transformed the treatment landscape for cancer and infectious disease through the investigational use of chimeric antigen receptor T-cells (CAR-Ts), tumour-infiltrating lymphocytes (TILs) and viral-specific T-cells (VSTs). Whilst these represent breakthrough treatments, there are subsets of patients who fail to respond to autologous ACT products. This is frequently due to impaired patient T-cell function or “fitness” as a consequence of prior treatments and age, and can be exacerbated by complex manufacturing protocols. Further, the manufacture of autologous, patient-specific products is time-consuming, expensive and non-standardised. Induced pluripotent stem cells (iPSCs) as an allogeneic alternative to patient-specific products can potentially overcome the issues outlined above. iPSC technology provides an unlimited source of rejuvenated iPSC-derived T-cells (T-iPSCs) or natural killer (NK) cells (NK-iPSCs), and in the context of the growing field of allogeneic ACT, iPSCs have enormous potential as a platform for generating off-the-shelf, standardised, “fit” therapeutics for patients. In this review, we evaluate current and future applications of iPSC technology in the CAR-T/NK, TIL and VST space. We discuss current and next-generation iPSC manufacturing protocols, and report on current iPSC-based adoptive therapy clinical trials to elucidate the potential of this technology as the future of ACT.
The Parasporal Body of Bacillus thuringiensis subsp. israelensis: A Unique Phage Capsid-Associated Prokaryotic Insecticidal Organelle
Authors: Rudd Sarah R. , Miranda Leticia Silva , Curtis Hannah R. , Bigot Yves , Diaz-Mendoza Mercedes , Hice Robert , Nizet Victor , Park Hyun-Woo , Blaha Gregor , Federici Brian A. , Bideshi Dennis K.
Published: November 2023

The three most important commercial bacterial insecticides are all derived from subspecies of Bacillus thuringiensis (Bt). Specifically, Bt subsp. kurstaki (Btk) and Bt subsp. aizawai (Bta) are used to control larval lepidopteran pests. The third, Bt subsp. israelensis (Bti), is primarily used to control mosquito and blackfly larvae. All three subspecies produce a parasporal body (PB) during sporulation. The PB is composed of insecticidal proteins that damage the midgut epithelium, initiating a complex process that results in the death of the insect. Among these three subspecies of Bt, Bti is unique as it produces the most complex PB consisting of three compartments. Each compartment is bound by a multilaminar fibrous matrix (MFM). Two compartments contain one protein each, Cry11Aa1 and Cyt1Aa1, while the third contains two, Cry4Aa1/Cry4Ba1. Each compartment is packaged independently before coalescing into the mature spherical PB held together by additional layers of the MFM. This distinctive packaging process is unparalleled among known bacterial organelles, although the underlying molecular biology is yet to be determined. Here, we present structural and molecular evidence that the MFM has a hexagonal pattern to which Bti proteins Bt152 and Bt075 bind. Bt152 binds to a defined spot on the MFM during the development of each compartment, yet its function remains unknown. Bt075 appears to be derived from a bacteriophage major capsid protein (MCP), and though its sequence has markedly diverged, it shares striking 3-D structural similarity to the Escherichia coli phage HK97 Head 1 capsid protein. Both proteins are encoded on Bti’s pBtoxis plasmid. Additionally, we have also identified a six-amino acid motif that appears to be part of a novel molecular process responsible for targeting the Cry and Cyt proteins to their cytoplasmic compartments. This paper describes several previously unknown features of the Bti organelle, representing a first step to understanding the biology of a unique process of sorting and packaging of proteins into PBs. The insights from this research suggest a potential for future applications in nanotechnology.
Iron Homeostasis in Azotobacter vinelandii
Authors: Rosa-Núñez Elena , Echavarri-Erasun Carlos , Armas Alejandro M. , Escudero Viviana , Poza-Carrión César , Rubio Luis M. , González-Guerrero Manuel
Published: November 2023

Iron is an essential nutrient for all life forms. Specialized mechanisms exist in bacteria to ensure iron uptake and its delivery to key enzymes within the cell, while preventing toxicity. Iron uptake and exchange networks must adapt to the different environmental conditions, particularly those that require the biosynthesis of multiple iron proteins, such as nitrogen fixation. In this review, we outline the mechanisms that the model diazotrophic bacterium Azotobacter vinelandii uses to ensure iron nutrition and how it adapts Fe metabolism to diazotrophic growth.
Detection and Quantification of Acrylamide in Second Trimester Amniotic Fluid Using a Novel LC-MS/MS Technique to Determine Whether High Acrylamide Content during Pregnancy Is Associated with Fetal Growth
Authors: Vrachnis Nikolaos , Loukas Nikolaos , Antonakopoulos Nikolaos , Maragou Niki , Kostakis Marios , Tsakni Aliki , Vrachnis Dionysios , Vougiouklaki Despina , Machairiotis Nikolaos , Chatzilazarou Arhodoula , Houhoula Dimitra , Sokou Rozeta , Stavros Sofoklis , Drakakis Peter , Mastorakos George , Iliodromiti Zoi
Published: November 2023

Introduction: Acrylamide, an organic compound, is, chemically speaking, a vinyl-substituted primary amide. It is produced industrially, principally as a precursor to polyacrylamides, for use in such products as plastics and cosmetics. This same compound, however, forms naturally in certain foods, both home-cooked and packaged, especially when prepared at high temperatures. We developed and validated a novel reliable technique for the determination of acrylamide in amniotic fluid. Multiple reaction monitoring (MRM) is a targeted mass spectrometry (MS) technique which enables the detection and quantification of particular molecules in a complex mixture. Thanks to its throughput, selectivity, and sensitivity, MRM-MS has been identified as offering an alternative to antibody-based studies for the purpose of biomarker verification. Our aim was to investigate the presence of acrylamide in amniotic fluid and, via the MRM-MS technique, to determine whether there is any correlation between maternal exposure to acrylamide, through a woman’s diet, and fetal growth. Methods: Our amniotic fluid bank included 40 samples from various fetal growth rates, as objectively denoted by the neonatal weight centile at delivery, while our analytical detection method was based on liquid chromatography-tandem mass spectrometry (LC-MS/MS). Acrylamide was determined with reversed phase chromatography and monitoring of two multiple reaction monitoring (MRM) transitions. Quantification was performed using the matrix-matched calibration curve. Results: Acrylamide was detected at concentrations between 7.1 and 1468 ng/mL in six out of the total of 40 amniotic fluid samples that were used. Our method limit of detection and quantification was 1.4 ng/mL and 4.6 ng/mL, respectively. The repeatability of our method ranged between 11 and 14%, expressed as relative standard deviation levels between 5 and 100 ng/mL. Conclusions: Detection of acrylamide in early second trimester amniotic fluid, for the first time in the literature to our knowledge, raises concerns about fetal health, given that published data on animal studies have attributed a number of birth defects to acrylamide. Our novel LC-MS/MS method for the determination of acrylamide in amniotic fluid proved to be effective and its performance in practice was very accurate, simple, and fast. Validation of the method revealed that the use of a matrix-matched curve is necessary for the quantification.
Types of Cell Death from a Molecular Perspective
Authors: Hajibabaie Fatemeh , Abedpoor Navid , Mohamadynejad Parisa
Published: November 2023

The former conventional belief was that cell death resulted from either apoptosis or necrosis; however, in recent years, different pathways through which a cell can undergo cell death have been discovered. Various types of cell death are distinguished by specific morphological alterations in the cell’s structure, coupled with numerous biological activation processes. Various diseases, such as cancers, can occur due to the accumulation of damaged cells in the body caused by the dysregulation and failure of cell death. Thus, comprehending these cell death pathways is crucial for formulating effective therapeutic strategies. We focused on providing a comprehensive overview of the existing literature pertaining to various forms of cell death, encompassing apoptosis, anoikis, pyroptosis, NETosis, ferroptosis, autophagy, entosis, methuosis, paraptosis, mitoptosis, parthanatos, necroptosis, and necrosis.
Influence of the Charge Ratio of Guanine-Quadruplex Structure-Based CpG Oligodeoxynucleotides and Cationic DOTAP Liposomes on Cytokine Induction Profiles
Authors: Le Nguyen Bui Thao , Tu Anh Thi Tram , Zhao Dandan , Yoshikawa Chiaki , Kawakami Kohsaku , Kaizuka Yoshihisa , Yamazaki Tomohiko
Published: November 2023

Cationic liposomes, specifically 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP) liposomes, serve as successful carriers for guanine-quadruplex (G4) structure-based cytosine-guanine oligodeoxynucleotides (CpG ODNs). The combined benefits of CpG ODNs forming a G4 structure and a non-viral vector carrier endow the ensuing complex with promising adjuvant properties. Although G4-CpG ODN-DOTAP complexes show a higher immunostimulatory effect than naked G4-CpG ODNs, the effects of the complex composition, especially charge ratios, on the production of the pro-inflammatory cytokines interleukin (IL)-6 and interferon (IFN)-α remain unclear. Here, we examined whether charge ratios drive the bifurcation of cytokine inductions in human peripheral blood mononuclear cells. Linear CpG ODN-DOTAP liposome complexes formed micrometer-sized positively charged agglomerates; G4-CpG ODN-DOTAP liposome complexes with low charge ratios (0.5 and 1.5) formed ~250 nm-sized negatively charged complexes. Notably, low-charge-ratio (0.5 and 1.5) complexes induced significantly higher IL-6 and IFN-α levels simultaneously than high-charge-ratio (2 and 2.5) complexes. Moreover, confocal microscopy indicated a positive correlation between the cellular uptake of the complex and amount of cytokine induced. The observed effects of charge ratios on complex size, surface charge, and affinity for factors that modify cellular-uptake, intracellular-activity, and cytokine-production efficiency highlight the importance of a rational complex design for delivering and controlling G4-CpG ODN activity.
A Systematic Review of Interleukins as Diagnostic and Prognostic Biomarkers for Peripheral Artery Disease
Authors: Djahanpour Niousha , Ahsan Naiyara , Li Ben , Khan Hamzah , Connelly Kim , Leong-Poi Howard , Qadura Mohammad
Published: November 2023

Background: Peripheral artery disease (PAD) involves atherosclerosis of the lower extremity arteries and is a major contributor to limb loss and death worldwide. Several studies have demonstrated that interleukins (ILs) play an important role in the development and progression of PAD; however, a comprehensive literature review has not been performed. Methods: A systematic review was conducted and reported according to PRISMA guidelines. MEDLINE was searched from inception to 5 December 2022, and all studies assessing the association between ILs and PAD were included. Results: We included 17 studies from a pool of 771 unique articles. Five pro-inflammatory ILs (IL-1β, IL-2, IL-5, IL-6, and IL-8) and one pro-atherogenic IL (IL-12) were positively correlated with PAD diagnosis and progression. In contrast, two anti-inflammatory ILs (IL-4 and IL-10) were protective against PAD diagnosis and adverse limb events. Specifically, IL-6 and IL-8 were the most strongly associated with PAD and can act as potential disease biomarkers to support the identification and treatment of PAD. Conclusions: Ongoing work to identify and validate diagnostic/prognostic inflammatory biomarkers for PAD has the potential to assist clinicians in identifying high-risk patients for further evaluation and management which could reduce the risk of adverse cardiovascular and limb events.
A New Theory about Interfacial Proton Diffusion Revisited: The Commonly Accepted Laws of Electrostatics and Diffusion Prevail
Authors: Knyazev Denis G. , Silverstein Todd P. , Brescia Stefania , Maznichenko Anna , Pohl Peter
Published: November 2023

The high propensity of protons to stay at interfaces has attracted much attention over the decades. It enables long-range interfacial proton diffusion without relying on titratable residues or electrostatic attraction. As a result, various phenomena manifest themselves, ranging from spillover in material sciences to local proton circuits between proton pumps and ATP synthases in bioenergetics. In an attempt to replace all existing theoretical and experimental insight into the origin of protons’ preference for interfaces, TELP, the “Transmembrane Electrostatically-Localized Protons” hypothesis, has been proposed. The TELP hypothesis envisions static H+ and OH− layers on opposite sides of interfaces that are up to 75 µm thick. Yet, the separation at which the electrostatic interaction between two elementary charges is comparable in magnitude to the thermal energy is more than two orders of magnitude smaller and, as a result, the H+ and OH− layers cannot mutually stabilize each other, rendering proton accumulation at the interface energetically unfavorable. We show that (i) the law of electroneutrality, (ii) Fick’s law of diffusion, and (iii) Coulomb’s law prevail. Using them does not hinder but helps to interpret previously published experimental results, and also helps us understand the high entropy release barrier enabling long-range proton diffusion along the membrane surface.
An Update on the Interplay between LRRK2, Rab GTPases and Parkinson’s Disease
Authors: Komori Tadayuki , Kuwahara Tomoki
Published: November 2023

Over the last decades, research on the pathobiology of neurodegenerative diseases has greatly evolved, revealing potential targets and mechanisms linked to their pathogenesis. Parkinson’s disease (PD) is no exception, and recent studies point to the involvement of endolysosomal defects in PD. The endolysosomal system, which tightly controls a flow of endocytosed vesicles targeted either for degradation or recycling, is regulated by a number of Rab GTPases. Their associations with leucine-rich repeat kinase 2 (LRRK2), a major causative and risk protein of PD, has also been one of the hot topics in the field. Understanding their interactions and functions is critical for unraveling their contribution to PD pathogenesis. In this review, we summarize recent studies on LRRK2 and Rab GTPases and attempt to provide more insight into the interaction of LRRK2 with each Rab and its relationship to PD.
Non-Coding RNAs as Potential Targets for Diagnosis and Treatment of Oral Lichen Planus: A Narrative Review
Authors: Kim Tae-Jun , Kim Yu Gyung , Jung Won , Jang Sungil , Ko Hyoung-Gon , Park Chan Ho , Byun Jin-Seok , Kim Do-Yeon
Published: November 2023

Oral lichen planus (OLP) is a chronic inflammatory disease that is characterized by the infiltration of T cells into the oral mucosa, causing the apoptosis of basal keratinocytes. OLP is a multifactorial disease of unknown etiology and is not solely caused by the malfunction of a single key gene but rather by various intracellular and extracellular factors. Non-coding RNAs play a critical role in immunological homeostasis and inflammatory response and are found in all cell types and bodily fluids, and their expression is closely regulated to preserve normal physiologies. The dysregulation of non-coding RNAs may be highly implicated in the onset and progression of diverse inflammatory disorders, including OLP. This narrative review summarizes the role of non-coding RNAs in molecular and cellular changes in the oral epithelium during OLP pathogenesis.
Impact of Portal Vein Resection (PVR) in Patients Who Underwent Curative Intended Pancreatic Head Resection
Authors: Bernhardt Markus , Rühlmann Felix , Azizian Azadeh , Kölling Max Alexander , Beißbarth Tim , Grade Marian , König Alexander Otto , Ghadimi Michael , Gaedcke Jochen
Published: November 2023

The oncological impact of portal vein resection (PVR) in pancreatic cancer surgery remains contradictory. Different variables might have an impact on the outcome. The aim of the present study is the retrospective assessment of the frequency of PVR, histological confirmation of tumor infiltration, and comparison of oncological outcomes in PVR patients. We retrieved n = 90 patients from a prospectively collected data bank who underwent pancreas surgery between 2012 and 2019 at the University Medical Centre Göttingen (Germany) and showed a histologically confirmed pancreatic ductal adenocarcinoma (PDAC). While 50 patients (55.6%) underwent pancreatic resection combined with PVR, 40 patients (44.4%) received standard pancreatic surgery. Patients with distal pancreatectomy or a tumor other than PDAC were excluded. PVR was performed either as local excision or circular resection of the portal vein. Clinical/patient data and follow-ups were retrieved. The median follow-up period was 20.5 months. Regarding the oncological outcome, a statistically poorer CSS (p = 0.04) was observed in PVR patients. There was no difference (p = 0.18) in patients’ outcomes between tangential and complete PVR, while n = 21 (42% of PVR patients) showed portal vein infiltration. The correlation between performed PVR and resection status was statistically significant: 48.6% of PVR patients achieved R0 resections compared to 75% in non-PVR patients (p = 0.03). Patients who underwent PDAC surgery with PVR show a significantly poorer outcome regardless of PVR type. Tumor size and R-status remain two important variables significantly associated with outcome. Since there is a lack of standardization for the indication of PVR, it remains unknown if the need for resection of vein structures during pancreatic resection represents the biological aggressiveness of the tumor or is biased by the experience of the surgeon.
Endocervical Adenocarcinoma Showing Microcystic, Elongated, and Fragmented (MELF) Pattern of Stromal Invasion: A Single-Institutional Analysis of 10 Cases with Comprehensive Clinicopathological Analyses and Ki-67 Immunostaining
Authors: Bae Hyunsik , Kim Hyun-Soo
Published: November 2023

Microcystic, elongated, and fragmented (MELF) pattern of invasion has seldom been documented in endocervical adenocarcinoma (EAC). The aim of this study was to analyze the clinicopathological characteristics of EAC showing MELF pattern. We collected the clinicopathological information of 10 cases of EAC with the MELF pattern and conducted polymer-based immunostaining for Ki-67 (dilution 1:200, clone MIB-1) on these cases. Ki-67 expression was assessed using the average estimation within the hotspot method. All tumors were human papillomavirus-associated EAC with Silva pattern C. All except one tumor exceeded 3 cm in size. Five tumors involved the entire thickness of the cervical stroma, and four tumors extended into the parametrium. Lymphovascular space invasion was identified in six cases. Two patients developed metastatic recurrences in the para-aortic lymph nodes and lungs, respectively. The MELF area showed significantly lower Ki-67 labelling index than that of a conventional tumor area. We confirmed our previous observation that the MELF area displayed lower proliferative activity than the conventional tumor area of EAC. We also demonstrated that patients with EAC showing MELF pattern had several adverse clinicopathological characteristics reflecting aggressive behavior. On the other hand, since the frequencies of post-operative recurrence and disease-related mortality that occurred during the follow-up period were relatively low, further investigations are warranted to clarify the prognostic value of MELF pattern in EAC patients.
Comparison of the Surface Roughness of CAD/CAM Metal-Free Materials Used for Complete-Arch Implant-Supported Prostheses: An In Vitro Study
Authors: Mory Nataly , Cascos Rocío , Celemín-Viñuela Alicia , Gómez-Polo Cristina , Agustín-Panadero Rubén , Gómez-Polo Miguel
Published: November 2023

The roughness of the intra-oral surfaces significantly influences the initial adhesion and the retention of microorganisms. The aim of this study was to analyze the surface texture of four different CAD-CAM materials (two high-performance polymers and two fifth-generation zirconia) used for complete-arch implant-supported prostheses (CAISPs), and to investigate the effect of artificial aging on their roughness. A total of 40 milled prostheses were divided into 4 groups (n = 10) according to their framework material, bio.HPP (B), bio.HPP Plus (BP), zirconia Luxor Z Frame (ZF), and Luxor Z True Nature (ZM). The areal surface roughness “Sa” and the maximum height “Sz” of each specimen was measured on the same site after laboratory fabrication (lab as-received specimen) and after thermocycling (5–55 °C, 10,000 cycles) by using a noncontact optical profilometer. Data were analyzed using SPSS version 28.0.1. One-way ANOVA with multiple comparison tests (p = 0.05) and repeated measures ANOVA were used. After thermocycling, all materials maintained “Sa” values at the laboratory as-received specimen level (p = 0.24). “Sz” increased only for the zirconia groups (p = 0.01). B-BP exhibited results equal/slightly better than ZM-ZF. This study provides more realistic surface texture values of new metal-free materials used in real anatomical CAISPs after the manufacturing and aging processes and establishes a detailed and reproducible measurement workflow.
The Value of Tumor Infiltrating Lymphocytes (TIL) for Predicting the Response to Neoadjuvant Chemotherapy (NAC) in Breast Cancer according to the Molecular Subtypes
Authors: Faur Ionut Flaviu , Dobrescu Amadeus , Clim Adelina Ioana , Pasca Paul , Prodan-Barbulescu Catalin , Gherle Bogdan Daniel , Tarta Cristi , Isaic Alexandru , Brebu Dan , Duta Ciprian , Totolici Bogdan , Lazar Gabriel
Published: November 2023

Introduction: The antitumor host immune response is an important factor in breast cancer, but its role is not fully established. The role of tumor infiltrating lymphocytes (TIL) as an immunological biomarker in breast cancer has been significantly explored in recent years. The number of patients treated with neoadjuvant chemotherapy (NAC) has increased and the identification of a biomarker to predict the probability of pCR (pathological complete response) is a high priority. Materials and methods: We evaluated 334 cases of BC treated with NAC followed by surgical resection from 2020–2022 at the Ist Clinic of Oncological Surgery, Oncological Institute “Prof Dr I Chiricuta” Cluj Napoca. Of the above, 122 cases were available for histological evaluation both in pre-NAC biopsy and post-NAC resection tissue. Evaluation of biopsy fragments and resection parts were performed using hematoxylin eosin (H&E). The TIL evaluation took place according to the recommendations of the International TIL Working Group (ITILWG). Results: There was a strong association between elevated levels of pre-NAC TIL. At the same time, there is a statistically significant correlation between stromal TIL and tumor grade, the number of lymph node metastases, the molecular subtype and the number of mitoses (p < 0.005). Intratumoral TIL showed a significant correlation with tumor size, distant metastasis, molecular subtype, number of mitosis, stage and lymph node metastasis (p < 0.005). We also demonstrated that high pre-NAC STIL represents a strong predictive marker for pCR. Conclusion: This study reveals the role of TIL as a predictive biomarker in breast cancer not only for the well-established TNBC (triple negative breast cancer) and HER2+ (Her2 overexpressed) subtypes but also in Luminal A and B molecular subtypes. In this scenario, the evaluation of sTIL as a novel predictive and therapy-predicting factor should become a routinely performed analysis that could guide clinicians when choosing the most appropriate therapy.