bluuebunny/crossref_metadata_2025 huggingface dataset:
Performance evaluation of reinforced earth walls with sustainable backfills subjected to railway loading
Authors: Mandloi Pushpraj , Hegde Amarnath
Published: October 2022

The concept of using Reinforced Earth (RE) walls for rail corridors is gaining popularity. As of now, very few nations have successfully adopted the RE walls for Railway infrastructure. These structures require good quality granular materials like sand for backfilling. However, due to the scarcity of good quality natural sand, research efforts are being made to look for cost effective alternatives to sand in backfill applications. The present study examines the possibilities of using the two sustainable materials, namely steel slag and construction and demolition waste (CDW), as backfills in RE walls subjected to harmonic train-induced load. In total, 63 separate full-scale numerical models of RE walls have been analyzed to understand the wall behavior with sustainable backfills subjected to different train speeds. The backfill materials were simulated with three different material models, namely, linear elastic, Mohr-Coulomb and Hardening Soil, for comparison. The results showed that the RE wall behavior is highly frequency-dependent. RE walls with steel slag and CDW backfills have shown 25% and 12% lesser deformations, respectively as compared to the sand backfill. Furthermore, reinforcement tensile forces in walls with sustainable backfills were found to be comparable to those of sand. The maximum deformation of the wall was observed when the fill materials were simulated with the Hardening Soil model. In overall, satisfactory performance of the RE wall was observed in the presence of sustainable backfill materials under Railway loading.
Bibliometric analysis of research trends in stem cell therapy for knee osteoarthritis over the period 2001–2021
Authors: Chen Runzhi , Jiang Yanyan , Lu Laiya , Wang Pei , Huang Dongya , Wang Jingyi , Liu Zheng , Qin Shaojie , Yin Feng
Published: October 2022

Stem cell therapy is a promising treatment for knee osteoarthritis, but few bibliometric studies have been performed on the subject. Bibliometric analysis is helpful for identifying the most influential studies in a specific field and can evaluate the global research trends in stem cell therapy for knee osteoarthritis. The Web of Science Core Collection was searched for publications from 2001 to 2021. Publication performance was analyzed using several bibliometric parameters, including VOSviewer, to identify the research landscape of trends in topics, and CiteSpace was investigated to identify the keywords that have the strongest citation bursts. From 2001 to 2021, in total, 1,345 publications explored the research on stem cells in knee osteoarthritis. The United States contributed the largest number of publications and at the top list of international collaborations. Tokyo Medical and Dental University ranked first among institutions in the overall number of articles and citations. The journal of Osteoarthritis and Cartilage had the largest number of publications. Sekiya Ichiro was the most cited author, with 32 articles. The keywords with the most frequent occurrence were “osteoarthritis,” “mesenchymal stem cells,” and “cartilage,” in descending order of frequency. “fibroblast growth factor” and “extracellular vesicle” were the first and last searched theme terms, respectively. The number of publications on stem cells for knee osteoarthritis stays growing. Cartilage repair and paracrine function are current research hotspots for the stem cell therapy mechanism. Stem cell therapy has gradually advanced from basic research to the clinical application stage.
New insights into Chlamydia pathogenesis: Role of leukemia inhibitory factor
Authors: Wang Jun , Wang Katherine
Published: October 2022

Chlamydia trachomatis (Ct)is the leading cause of bacterial sexually transmitted infections worldwide. Since the symptoms ofCtinfection are often subtle or absent, most people are unaware of their infection until they are tested or develop severe complications such as infertility. It is believed that the primary culprit ofCt-associated tissue damage is unresolved chronic inflammation, resulting in aberrant production of cytokines, chemokines, and growth factors, as well as dysregulated tissue influx of innate and adaptive immune cells. A member of the IL-6 cytokine family, leukemia inhibitory factor (LIF), is one of the cytokines induced byCtinfection but its role inCtpathogenesis is unclear. In this article, we review the biology of LIF and LIF receptor (LIFR)-mediated signaling pathways, summarize the physiological role of LIF in the reproductive system, and discuss the impact of LIF in chronic inflammatory conditions and its implication inCtpathogenesis. Under normal circumstances, LIF is produced to maintain epithelial homeostasis and tissue repair, including the aftermath ofCtinfection. However, LIF/LIFR-mediated signaling – particularly prolonged strong signaling – can gradually transform the microenvironment of the fallopian tube by altering the fate of epithelial cells and the cellular composition of epithelium. This harmful transformation of epithelium may be a key process that leads to an enhanced risk of infertility, ectopic pregnancy and cancer followingCtinfection.
Enzootic Trypanosoma cruzi infection by Rhodnius prolixus shows transmission to humans and dogs in Vichada, Colombia
Authors: Cantillo-Barraza Omar , Solis Cesil , Zamora Alexander , Herazo Rafael , Osorio María Isabel , Garcés Edilson , Xavier Samanta , Mejía-Jaramillo Ana María , Triana-Chávez Omar
Published: October 2022

Background

Rhodnius prolixus is considered the most relevant Trypanosoma cruzi vector in Colombia and Venezuela due it is responsible for domestic transmission in both countries. However, a wild population of this species is distributed in the eastern plains of the Orinoco region and Amazonia jungle, where its epidemiological importance has not been sufficiently elucidated. This study aimed to assess epidemiological parameters of T. cruzi transmission in the Department of Vichada, Colombia.

Methods

We determined the characteristics of T. cruzi transmission using entomological studies in domestic and sylvatic ecotopes. We analyzed the T. cruzi infection in triatomine insects, identified blood meal sources, and conducted a serological determination of T. cruzi infection in scholar-aged children, domestic dogs, and wild hosts.

Results

Fifty-four triatomine bugs, 40 T. maculata and 14 R. prolixus were collected in peridomestic and sylvatic ecotopes. Infected R. prolixus was observed in La Primavera, Santa Rosalia, and Cumaribo municipalities. All the T. maculata bugs were not infected. Serological analysis indicated that two of 3,425 children were T. cruzi positive. The seroprevalence in domestic dogs was 10,5% (49/465). Moreover, 22 synanthropic mammals were sampled, being Didelphis marsupialis the most common. TcI genotype was detected in seropositive dogs, R. prolixus, and D. marsupialis.

Conclusion

The present work describes extra domestic R. prolixus and D. marsupialis in a sylvatic T. cruzi transmission cycle with transmission to humans and domestic dogs in Colombia’s Vichada Department.
Uterine fibroids are associated with increased risk of pre-eclampsia: A case-control study
Authors: Gong Lina , Liu Meng , Shi Haiheng , Huang Ying
Published: October 2022

Purpose

Uterine fibroids are associated with hypertension in non-pregnant women. We aimed to evaluate the association between uterine fibroids and pre-eclampsia (PE).

Patients and methods

Participants were pregnant women who delivered in the Department of Obstetrics of the People's Hospital of Xinjiang Uygur Autonomous Region between January and December 2021. Patients with PE were identified as the case group, whereas those without PE were selected as the control group, using age-matching and a ratio of 1:5. Ultrasound examination during early pregnancy was used to detect uterine fibroids. Multivariable logistic regression was applied to evaluate the association between uterine fibroids and PE.

Results

In total, 121 cases with PE and 578 controls without PE were included, with mean age of 32.9 years and gestational age of 37.7 weeks. Time of ultrasound examination was 12.0 ± 2.6 weeks. The case group had a significantly higher exposure rate of uterine fibroids than the control group (14.0 vs. 6.9%, P = 0.009). Multivariable Logistic regression models adjusted for potential confounding factors, including gestational age and blood pressure in early gestation, showed that pregnant women with uterine fibroids in early pregnancy exhibited three-fold higher odds for PE (OR, 3.02; 95% CI, 1.20–7.60; P = 0.019). Sensitivity analysis, which excluded those with gestational diabetes, further confirmed the robustness of the results. The association between uterine fibroids and PE was stronger in pregnant women aged ≥35 years and multiparas.

Conclusion

Uterine fibroids are significantly associated with an increased risk of PE in pregnant women. Uterine fibroids may serve as a new factor for identifying pregnant women at high risk of PE, and the effect of myomectomy before pregnancy on prevention of PE is worth further exploring.
Impact of late gadolinium enhancement extent, location, and pattern on ventricular tachycardia and major adverse cardiac events in patients with ischemic vs. non-ischemic cardiomyopathy
Authors: Tat Emily , Ball Caroline , Camren Gerald P. , Wroblewski Igor , Dajani Khaled A. , Goldberg Ari , Kinno Menhel , Sanagala Thriveni , Syed Mushabbar A. , Wilber David J. , Rabbat Mark
Published: October 2022

Background

Left ventricular late gadolinium enhancement (LGE) by cardiac magnetic resonance (CMR) has been associated with increased risk for life-threatening ventricular tachyarrhythmias. The differences in association between LGE characteristics and prognosis in patients with ischemic (ICM) vs. non-ischemic (NICM) cardiomyopathy is incompletely understood.

Methods

A total of 168 consecutive patients who underwent CMR imaging with either ICM or NICM were included in our study. LGE extent, location and pattern were examined for association to the primary endpoint of ventricular tachycardia (VT) and secondary endpoint of major adverse cardiac events (MACE).

Results

Of 68 (41%) patients with ICM and 97 (59%) patients with NICM, median LGE mass was 15% (IQR 9–28) for the ICM group and 10% (IQR 6–15) for the NICM group. On multivariate analysis for both groups, LGE characteristics were prognostic while LVEF was not. In patients with ICM, septal and apical segment LGE, and involvement of multiple walls predicted both endpoints on multivariate analysis. LGE extent (≥median) and inferior wall LGE independently predicted the primary endpoint. In patients with NICM, anterior, inferior and apical segment LGE, and involvement of multiple walls predicted both endpoints on multivariate analysis. LGE extent (≥median, number of LGE segments, LGE stratified per 5% increase) and midwall LGE were independent predictors of the primary endpoint.

Conclusions

Although LGE was an independent predictor of prognosis in both groups, LGE extent, location, and pattern characteristics were more powerful correlates to worse outcomes in patients with NICM than ICM.
Case report: Efficacy analysis of radiofrequency catheter ablation combined with atrial appendage resection for atrial tachycardia originating from the atrial appendage in children
Authors: Liu Jing , Cao Xiaoxiao , Li Changjian , Feng Xiaoyuan , Sun Dongming , Zhang Yong
Published: October 2022

Objective

The aim of this study was to investigate the efficacy of radiofrequency catheter ablation (RFCA) combined with atrial appendage (AA) resection to treat atrial tachycardia (AT) originating from the AA in children.

Materials and methods

Using the Ensite three-dimensional electroanatomic mapping system, three children with AT originating from the AA were diagnosed. Clinical features and electrocardiographic (ECG) manifestations were analyzed. Ablations were performed using a cold saline-infused catheter at appendages targeting loci of AT origin under the guidance of the Ensite system. Atrial appendage resection was performed in combination with cardiac surgery, and the curative effect was evaluated.

Results

The ages of the three patients were 3.5, 5.75, and 12.9 years. Two cases originated from the right atrial appendage (RAA) and one originated from the left atrial appendage (LAA). The ECG characteristics of AT from the RAA were as follows: (1) negative P waves in lead V1; (2) positive P waves in leads II, III, and aVF; (3) positive P wave in lead I with varying shapes in lead aVL; and (4) prolonged PR interval with no QRS wave after some P waves. The ECG of the LAA was characterized by (1) positive P waves in lead V1 with a bimodal pattern; (2) positive P waves in leads II, III, and aVF; and (3) negative P waves in leads I and aVL. Preoperative echocardiography showed cardiac enlargement and a decreased left ventricular ejection fraction (LVEF) in all three cases. One case was cured after RFCA, and the remaining two cases required AA resection after RFCA. No recurrence was detected at 1–18 months of follow-up, and the left ventricular end-diastolic diameter and LVEF returned to normal.

Conclusion

Atrial tachycardia originating from the AA in children showed a characteristic P-wave presentation on ECG, and sustained episodes of AT resulted in tachycardia-induced cardiomyopathy. Children who are not successfully controlled by RFCA or who have a recurrence after RFCA could benefit from AA resection.
Utilizing digital predictive biomarkers to identify Veteran suicide risk
Authors: Holmgren Jackson G. , Morrow Adelene , Coffee Ali K. , Nahod Paige M. , Santora Samantha H. , Schwartz Brian , Stiegmann Regan A. , Zanetti Cole A.
Published: October 2022

Veteran suicide is one of the most complex and pressing health issues in the United States. According to the 2020 National Veteran Suicide Prevention Annual Report, since 2018 an average of 17.2 Veterans died by suicide each day. Veteran suicide risk screening is currently limited to suicide hotlines, patient reporting, patient visits, and family or friend reporting. As a result of these limitations, innovative approaches in suicide screening are increasingly garnering attention. An essential feature of these innovative methods includes better incorporation of risk factors that might indicate higher risk for tracking suicidal ideation based on personal behavior. Digital technologies create a means through which measuring these risk factors more reliably, with higher fidelity, and more frequently throughout daily life is possible, with the capacity to identify potentially telling behavior patterns. In this review, digital predictive biomarkers are discussed as they pertain to suicide risk, such as sleep vital signs, sleep disturbance, sleep quality, and speech pattern recognition. Various digital predictive biomarkers are reviewed and evaluated as well as their potential utility in predicting and diagnosing Veteran suicidal ideation in real time. In the future, these digital biomarkers could be combined to generate further suicide screening for diagnosis and severity assessments, allowing healthcare providers and healthcare teams to intervene more optimally.
A randomized controlled trial to pilot the efficacy of a computer-based intervention with elements of virtual reality and limited therapist assistance for the treatment of post-traumatic stress disorder
Authors: van Meggelen Marieke , Morina Nexhmedin , van der Heiden Colin , Brinkman Willem-Paul , Yocarini Iris E. , Tielman Myrthe L. , Rodenburg Jan , van Ee Elisa , van Schie Kevin , Broekman Marijke E. , Franken Ingmar H. A.
Published: October 2022

Although well-established therapies exist for post-traumatic stress disorder (PTSD), barriers to seek mental health care are high. Technology-based interventions may play a role in improving the reach of efforts to treat, especially when therapist availability is low. The goal of the current randomized controlled trial was to pilot the efficacy of a computer-based trauma intervention with elements of virtual reality (VR; 3MR system) and limited therapist involvement for the treatment of PTSD in a childhood sexual abuse (CSA) and war veteran sample and to compare this to “treatment as usual” (TAU). TAU consisted of evidence-based approaches such as imaginal exposure, EMDR, or narrative exposure therapy. A total of 44 patients with PTSD were included and randomly assigned to 12 sessions of 3MR intervention or TAU (completer n 3MR = 12, TAU = 18). Several measures (PCL-5, BDI-II, OQ-45-2, and the M.I.N.I. 5.0.0.) were administered to measure symptoms of PTSD and depression and scores of overall well-being at pre, post, and a three-month follow-up measurement. Analyses suggest that symptoms of PTSD and depression in the 3MR condition decreased, and overall well-being increased between pre and post measurements. Results did not indicate any clear differences between the treatment conditions over time which suggests that treatment gains of the 3MR intervention seem no less than those of TAU. Finally, both treatment conditions produced similar remission rates of PTSD and depression. Therefore, the 3MR intervention could possibly constitute an appropriate treatment alternative. The small sample size as well as evident drop-out rates in the 3MR condition (45%) do warrant further research. The procedures of this study were approved by the Medical Ethical Research Committee (MERC) of the Erasmus Medical Center in Rotterdam (MEC-NL46279.078.13) and pre-registered via ClinicalTrials.gov (Protocol Record CI1-12-S028-1).
Mapping SS1-3 chemistry teachers’ interest, self-efficacy, and literacy in teaching for creativity using simulation
Authors: Nja Cecilia Obi , Ukwetang John Okpa , Orim Richard Ekonesi , Cornelius-Ukpepi Bernedette , Ndifon Rita Asu
Published: October 2022

The purpose of this article was to analyze chemistry teachers’ interest, literacy, self-efficacy, teamwork, and creativity in the use of simulation teaching of concepts in chemistry to enhance students’ creativity skills. A descriptive and exploratory quantitative design was used in the study. The study used 150 serving chemistry teachers from the Calabar education zone public education system. A 29-item questionnaire was employed to gather data from respondents. Independentt-test, regression, and a 2-way analysis of variance were used for data analysis. Results obtained indicated high levels of teachers’ interest, literacy, self-efficacy, and teamwork in the utilization of simulation strategy in teaching chemistry concepts. Results of a 2-way analysis indicated that teachers’ age and years of experience influence teachers’ self-efficacy, teamwork, and creativity. It was recommended among others that serving teachers’ interest in the use of simulation is encouraged to support their learners’ instructional activities in a simulated classroom.
Effect of 131I with and without artificial liver support system in patients with Graves’ disease and severe liver dysfunction: A retrospective study
Authors: Rao Maohua , Wang Yirui , Ren Jianli , Chen Yue , Zheng Chenxi , Xiong Yalan , Yan Qingbo , Li Shiying , Yuan Gengbiao
Published: October 2022

Objective

Treatment decision-making in Graves’ disease (GD) with severe liver dysfunction (LD) is a clinical challenge. This research was carried out to evaluate the effect of radioiodine (131I) with or without an artificial liver support system (ALSS) in GD patients with severe LD.

Methods

In total, 45 patients diagnosed with GD and severe LD were enrolled and allocated to two groups: patients treated with 131I alone (n=30) (Group A)and patients by a combination of 131I and ALSS (n=15)(Group B). Liver function, thyroid hormone concentrations, therapeutic efficacy, and the cost of treatment were compared between the two groups.

Results

Thyroid hormone concentrations were lower 2 weeks after 131I treatment, but no deterioration in liver function was identified. There was no statistically significant difference in the treatment efficacy between the two groups. The hospital stay, total cost, and daily cost were lower in patients treated with 131I alone than in those treated with 131I and an ALSS (p<0.05).

Conclusion

The key point of treating GD patients with severe LD is to control the GD.131I is recommended as an effective and safe and should be applied as soon as possible once the diagnosis is clarified; however, when used in combination with an ALSS, there was no substantial improvement in therapeutic efficacy.
Investigating the relationship between the skin microbiome and flame retardant exposure of the endangered St. Lawrence Estuary beluga
Authors: Jia Baofeng , Garlock Emma , Allison Michael J. , Michaud Robert , Lo Raymond , Round Jessica M. , Helbing Caren C. , Verreault Jonathan , Brinkman Fiona S. L.
Published: October 2022

The endangered beluga (Delphinapterus leucas) population in the St. Lawrence Estuary (SLE) in eastern Canada, the largest estuary in the world, is declining. Elevated tissue concentrations of a wide range of environmental contaminants, for example, halogenated flame retardants (HFRs) including polybrominated diphenyl ethers (PBDEs), might play a role in the non-recovery of this population. In mammals, HFRs have been reported to impair the metabolic regulation, including amino acid and fatty acid pathways. In the present study, we collected both blubber and skin swab samples from tissue biopsies from 56 adult SLE belugas and analyzed their blubber for the concentrations of a comprehensive suite of PBDEs and other HFRs. Using 16S rRNA marker and shotgun metagenomic approaches using skin swabs, we investigated, for the first time, the SLE beluga skin microbiome and the SLE water microbiome, providing valuable comparative taxonomic and functional microbiome information. We found that belugas have a unique skin microbiome that is distinct from surrounding SLE water, regardless of the beluga sex or location in the SLE. We further characterized the core microbiome of SLE beluga skin and surrounding SLE water, and identified bacterial taxa and gene functional pathways associated with the skin microbiome that correlated with beluga blubber HFR concentrations. Namely, we identified the phylum Nitrospinae and candidate phylum PAUC34f as potential taxa of interest that are associated with blubber HFR concentrations. We hypothesize that the biodegradation of HFRs within the beluga blubber and skin results in an increase in local metabolite concentrations that leads to the proliferation of Nitrospinae and PAUC32f. This work demonstrates the utility of studying the core microbiome of the SLE beluga skin using a swab method that could be adapted to field sampling. Further studies of the temporal effects of contaminant exposure on SLE beluga skin and SLE water microbiomes is warranted for potentially better monitoring and protecting this marine mammal which is at risk.
Environmental regulation, agricultural green technology innovation, and agricultural green total factor productivity
Authors: Sun Yongchun
Published: October 2022

In order to promote the green transformation of agricultural development, we used a partial linear function coefficient panel model to measure the impact of environmental regulations in 30 provinces and cities in China on agricultural green technology innovation and agricultural green total factor productivity. The advantage of this model is that it can take into account the heterogeneity of regional economic development levels, that is, by introducing variables that are functions of regional economic development levels as coefficients of environmental regulation. The research results show that: when the level of regional economic development is low, environmental regulation has a limited impact on agricultural green technology innovation and agricultural green total factor productivity, but as the level of regional economic development gradually increases, environmental regulation has a more significant impact on the two. And environmental regulation has a greater impact on agricultural green total factor productivity than on agricultural green technology innovation. Based on the research results, policy recommendations are suggested.
COVID-19 in India: Epidemiological reflections from initial 170 million consecutive test results
Authors: Lohia Rohan , Goel Prabudh , Kaur Jasmine , Kumar Sujeet , Bajpai Minu , Singh Harpreet
Published: October 2022

Background

The Indian Council of Medical Research (ICMR) played a crucial role in streamlining testing and diagnosis, formulating guidelines, and devising management strategies during the COVID-19 pandemic. Additionally, ICMR designed and developed a comprehensive data management tool for collecting testing data in a standardized format from all laboratories across the country. The current report is a retrospective analysis of the testing data generated by the ICMR. The study's main objectives are to understand the probability of a person testing negative based on their age after an initial positive test and to assess the varied impact and duration of the disease in people of different age groups and genders.

Methods

Anonymized data on the testing for COVID were analyzed. The P-to-P is the longest time interval between two consecutive positive tests for a patient without any negative test in between the positives. P-to-Plast is the time between the first positive and last positive test, as opposed to P-to-P, here we are looking at the first and last positive tests that might or might not be consecutive. P-to-N intervals is the time between the first positive and first negative test of a patient.

Results

India conducted 170,914,170 tests during the study-period (until December 29, 2020). After excluding invalid test results and duplicates, there were 11,101,603 (6.5%) positive and 156,542,352 (93.5%) negative test-results performed upon 150,086,257 unique individuals. A negative-report following a positive-test was available in 12.69%. Nearly three-fourths of the cases (78.29%) belonged to the working-age group (18–60 years). The proportion of patients >50 years old has risen from 26.06 to 35.03%, with a steep rise beyond September 2020. Gender-ratio among the positives was 1.73:1 which was neutral in neonates < 7-days (age). The gender ratio was skewed in-favor-of males in the initial months with a reverse trend thereafter and with increasing age of patients. The mean P-to-P, P-to-Plast, and P-to-N durations were 12.7 + 4.3, 13.3 + 4.6, and 14.2 + 4.9 days for individuals with P-to-P duration of 1–4 weeks. The probability of testing negative was 82 & 85% at 14 & 21 days after the first-positive-test respectively with no gender bias.

Conclusions

The current study has highlighted some vital aspects of COVID-19 epidemiology in India. This study will add to the current understanding of the virus in the absence of pre- existing information on the novel virus and the disease per se.
Genetic analysis of digital image derived morphometric traits of black tiger shrimp (Penaeus monodon) by incorporating G × E investigations
Authors: Hasan Md. Mehedi , Thomson Peter C. , Raadsma Herman W. , Khatkar Mehar S.
Published: October 2022

The black tiger shrimp, Penaeus monodon, is the second most economically important aquaculture shrimp species in the world, and in Australia it is one of the most commonly farmed shrimp species. Despite its economic significance, very few studies have reported the genetic evaluation of economically important morphological size and shape traits of shrimp grown in commercial grow-out environments. In this study we obtained genetic parameter estimates and evaluated genotype-by-environment interaction (GxE) for nine body morphological traits of shrimp derived from images. The data set contained image and body weight (BW) records of 5,308 shrimp, from 64 sires and 54 dams, reared in eight grow-out ponds for an average of 133 days. From the images, landmark based morphological distances were computed from which novel morphological traits associated with size and shape were derived for genetic evaluation. These traits included body weight (BW), body length (BL), body size (BS), head size (HS), Abdominal size (AS), abdominal percentage (AP), tail tip (TT), front by back ratio (FBR), condition factor (CF) and condition factor length (CFL). We also evaluated G×E interaction effects of these traits for shrimp reared in different ponds. The heritability estimates for growth related morphological and body weight traits were moderately high (BW: h2 = 0.32 ± 0.05; BL: h2 = 0.36 ± 0.06; BS: h2 = 0.32 ± 0.05; HS: h2 = 0.31 ± 0.05; AS: h2 = 0.32 ± 0.05; and TT: h2 = 0.28 ± 0.05) and low for abdominal percentage and body shape traits (AP: h2 = 0.09 ± 0.02; FBR: h2 = 0.08 ± 0.02; CF: h2 = 0.06 ± 0.02; and CFL: h2 = 0.003 ± 0.004). G × E interaction were negligible for all traits for shrimp reared in different ponds, suggesting re-ranking is not prevalent for this population. Genetic correlations among growth related morphological traits were high ranging from 0.36 to 0.99, suggesting these traits can be simultaneously improved through indirect genetic selection. However, negative genetic correlations were observed for FBR & CF shape traits with major growth traits (ranged −0.08 to −0.95), suggesting genetic selection for rapid growth will likely result in thick/fatty shrimp over generations. Our study showed image-based landmark data can be successfully employed for genetic evaluation of complex morphological traits of shrimp and is potentially amenable to machine-learning derived parameters in semi-automated high volume phenotyping systems needed under commercial conditions.
Fusion primer driven racket PCR: A novel tool for genome walking
Authors: Pei Jinfeng , Sun Tianyi , Wang Lingqin , Pan Zhenkang , Guo Xinyue , Li Haixing
Published: October 2022

The limitations of the current genome-walking strategies include strong background and cumbersome experimental processes. Herein, we report a genome-walking method, fusion primer-driven racket PCR (FPR-PCR), for the reliable retrieval of unknown flanking DNA sequences. Four sequence-specific primers (SSP1, SSP2, SSP3, and SSP4) were sequentially selected from known DNA (5'→3′) to perform FPR-PCR. SSP3 is the fragment that mediates intra-strand annealing (FISA). The FISA fragment is attached to the 5′ end of SSP1, generating a fusion primer. FPR-PCR comprises two rounds of amplification reactions. The single-fusion primary FPR-PCR begins with the selective synthesis of the target first strand, then allows the primer to partially anneal to some place(s) on the unknown region of this strand, producing the target second strand. Afterward, a new first strand is synthesized using the second strand as the template. The 3′ end of this new first strand undergoes intra-strand annealing to the FISA site, followed by the formation of a racket-like DNA by a loop-back extension. This racket-like DNA is exponentially amplified in the secondary FPR-PCR performed using SSP2 and SSP4. We validated this FPR-PCR method by identifying the unknown flanks of Lactobacillus brevis CD0817 glutamic acid decarboxylase genes and the rice hygromycin gene.
Exploring the immunomodulatory role of virtual memory CD8+ T cells: Role of IFN gamma in tumor growth control
Authors: Savid-Frontera Constanza , Viano Maria Estefania , Baez Natalia S. , Lidon Nicolas L. , Fontaine Quentin , Young Howard A. , Vimeux Lene , Donnadieu Emmanuel , Rodriguez-Galan Maria Cecilia
Published: October 2022

Virtual memory CD8+T cells (TVM) have been described as cells with a memory-like phenotype but without previous antigen (Ag) exposure. TVMcells have the ability to respond better to innate stimuli rather than by TCR engagement, producing large amounts of interferon gamma (IFNγ) after stimulation with interleukin (IL)-12 plus IL-18. As a result of the phenotypic similarity, TVMcells have been erroneously included in the central memory T cell subset for many years. However, they can now be discriminatedviathe CD49d receptor, which is up-regulated only on conventional memory T cells (TMEM) and effector T cells (TEFF) after specific cognate Ag recognition by a TCR. In this work we show that systemic expression of IL-12 plus IL-18 induced an alteration in the normal TVMvs TMEM/TEFFdistribution in secondary lymphoid organs and a preferential enrichment of TVMcells in the melanoma (B16) and the pancreatic ductal adenocarcinoma (KPC) tumor models. Using our KPC bearing OT-I mouse model, we observed a significant increase in CD8+T cell infiltrating the tumor islets after IL-12+IL-18 stimulation with a lower average speed when compared to those from control mice. This finding indicates a stronger interaction of T cells with tumor cells after cytokine stimulation. These results correlate with a significant reduction in tumor size in both tumor models in IL-12+IL-18-treated OT-I mice compared to control OT-I mice. Interestingly, the absence of IFNγ completely abolished the high antitumor capacity induced by IL-12+IL-18 expression, indicating an important role for these cytokines in early tumor growth control. Thus, our studies provide significant new information that indicates an important role of TVMcells in the immune response against cancer.
Dispersal of juvenile leatherback turtles from different Caribbean nesting beaches: A model study
Authors: Gaspar Philippe , Candela Tony , Shillinger George L.
Published: October 2022

The Northwest Atlantic (NWA) leatherback turtle (Dermochelys coriacea) subpopulation was recently classified as endangered. It nests in the Wider Caribbean Region and includes five genetic stocks, all declining, albeit at different rates. The causes of decline are multiple and difficult to identify based on annual nest counts which integrate the effects of multiple stressors over the entire life history. Demographic models, however, show that survival during the juvenile pelagic stage is the main factor modulating population trends, but this life stage remains largely unobserved. This paper presents a suite of numerical simulations where juveniles from the five NWA stocks disperse under the combined effects of ocean currents and habitat-driven swimming movements. Simulations reveal when and where NWA juveniles likely disperse and, thus, the environmental conditions and anthropogenic threats they may encounter. Simulated individuals initially disperse following either the “Caribbean route,” inside the Caribbean Sea and the Gulf of Mexico (GoM), or the “Atlantic route” east of the Antilles Islands Arc. The percentage of individuals following one or the other route varies markedly with the stock of origin. Late dispersal in the eastern Atlantic Ocean and the Mediterranean Sea is similar in all stocks. Juveniles following the Caribbean route are rapidly entrained northwards by the Gulf Stream and incur a high risk of cold-induced mortality. This mostly affects the Florida stock and the Western Caribbean (WCA) stock nesting in Costa Rica, Panama, and Colombia. The Atlantic route is less lethal as individuals progress more slowly toward higher latitudes. Simulations also show that the percentage of WCA juveniles visiting the GoM is larger than for any other stock. The learned migration goal (LMG) hypothesis, which posits that adult sea turtles tend to exploit foraging areas previously identified at the juvenile stage, may thus explain why WCA adults are overrepresented in the GoM. Finally, our results suggest that the recently observed increase in the percentage of WCA adults migrating into the GoM could be linked to bycatch reduction measures implemented in 2003–2004, combined with an increase in the frequency of Loop Current intrusion and eddy-shedding events that started around the year 2000.
Phosphorylation of S6RP in peritubular capillaries of kidney grafts and circulating HLA donor-specific antibodies
Authors: Raïch-Regué Dalia , Gimeno Javier , Llinàs-Mallol Laura , Menéndez Silvia , Benito David , Redondo Dolores , Pérez-Sáez M. José , Riera Marta , Reed Elaine F. , Pascual Julio , Crespo Marta
Published: October 2022

Antibody-mediated rejection (ABMR) caused by donor-specific HLA-antibodies (DSA) is a mediator of allograft loss after kidney transplantation (KT). DSA can activate microvascular endothelium damage through the mTOR pathway. In this study we assessed the mTOR pathway activation by DSA in KT with ABMR (ABMR + DSA+) compared to controls (ABMR−DSA−), biopsies with ABMR changes without DSA (ABMR + DSA−) and DSA without ABMR changes (ABMR−DSA+), and the potential modulation by mTOR inhibitors (mTORi). We evaluated 97 biopsies: 31 ABMR + DSA+, 33 controls ABMR-DSA−, 16 ABMR + DSA−, and 17 ABMR-DSA+ cases. Regarding immunosuppression of full ABMR + DSA+ and controls, 21 biopsies were performed under mTORi treatment (11 of them ABMR + DSA+ cases) and 43 without mTORi (20 of them ABMR + DSA+) so as to explore its effect on the mTOR pathway. Biopsies were stained for C4d, Ki67, and phosphorylated (p) S6RP, ERK, and mTOR by immunohistochemistry. Labeling was graded according to peritubular capillary staining. ABMR biopsies showed significantly higher C4d, p-S6RP, and Ki67 staining in peritubular capillaries (PTC) compared to controls, and light differences in p-ERK or p-mTOR. mTORi treatment did not modify p-S6RP, p-mTOR, and p-ERK staining. Diffuse p-S6RP in PTC in the biopsies significantly associated with circulating HLA-DSA independently of graft rejection, and with worse death-censored graft survival. These findings suggest that activation of endothelium through the mTOR pathway evidence different mechanisms of damage in ABMR + DSA+ and ABMR + DSA− despite similar histological injury.
Off-label use of canakinumab in pediatric rheumatology and rare diseases
Authors: Del Giudice Emanuela , Sota Jurgen , Orlando Francesca , Picciano Ludovica , Cimaz Rolando , Cantarini Luca , Mauro Angela
Published: October 2022

Since the first success of interleukin-1 blockade in cryopyrin-associated periodic syndrome, the use of interleukin-1 inhibitors has expanded to other disorders, including off-label indications. In particular, canakinumab has been employed in an off-label fashion in several diseases such as rare monogenic autoinflammatory diseases and multifactorial autoinflammatory diseases, disclosing an excellent efficacy and good safety profile in pediatric patients unresponsive to standards of care. In addition, hyperferritinemic syndromes and complex disorders, as well as Kawasaki disease, uveitis, and other pediatric rare disorders, represent additional areas where canakinumab efficacy is worth exploring. Altogether, the results summarized below are of paramount importance in pediatric patients where a considerable proportion of treatments are prescribed off-label. This review focuses on the off-label use of canakinumab in pediatric patients affected by systemic immune-mediated diseases.
GC-MS profiling of volatile metabolites produced by Klebsiella pneumoniae
Authors: Filipiak Wojciech , Żuchowska Karolina , Marszałek Marta , Depka Dagmara , Bogiel Tomasz , Warmuzińska Natalia , Bojko Barbara
Published: October 2022

Currently used methods for diagnosing ventilator-associated pneumonia (VAP) are complex, time-consuming and require invasive procedures while empirical antibacterial therapy applies broad spectrum antibiotics that may promote antimicrobial resistance. Hence, novel and fast methods based on alternative markers are needed for VAP detection and differentiation of causative pathogens. Pathogenic bacteria produce a broad range of volatile organic compounds (VOCs), some of which may potentially serve as biomarkers for microorganism identification. Additionally, monitoring of dynamically changing VOCs concentration profiles may indicate emerging pneumonia and allow timely implementation of appropriate antimicrobial treatment. This study substantially extends the knowledge on bacterial metabolites providing the unambiguous identification of volatile metabolites produced by carbapenem-resistant and susceptible strains of Klebsiella pneumoniae (confirmed with pure standards in addition to mass spectra match) but also revealing their temporary concentration profiles (along the course of pathogen proliferation) and dependence on the addition of antibiotic (imipenem) to bacteria. Furthermore, the clinical strains of K. pneumoniae isolated from bronchoalveolar lavage specimens collected from mechanically ventilated patients were investigated to reveal, whether bacterial metabolites observed in model experiments with reference strains could be relevant for wild pathogens as well. In all experiments, the headspace samples from bacteria cultures were collected on multibed sorption tubes and analyzed by GC-MS. Sampling was done under strictly controlled conditions at seven time points (up to 24 h after bacteria inoculation) to follow the dynamic changes in VOC concentrations, revealing three profiles: release proportional to bacteria load, temporary maximum and uptake. Altogether 32 VOCs were released by susceptible and 25 VOCs by resistant strain, amongst which 2-pentanone, 2-heptanone, and 2-nonanone were significantly higher for carbapenem-resistant KPN. Considerably more metabolites (n = 64) were produced by clinical isolates and in higher diversity compared to reference KPN strains.
Biochemical profiling of metabolomics in heavy metal-intoxicated impaired metabolism and its amelioration using plant-based bioactive compound
Authors: Yaqoob Azka , Rehman Kanwal , Akash Muhammad Sajid Hamid , Alvi Maria , Shoaib Syed Muhammad
Published: October 2022

Exposure to Pb is widely spreading and has far-reaching negative effects on living systems. This study aimed to investigate the toxic effects of Pb, through biochemical profiling and the ameliorative effects of quercetin against Pb-toxicity. Twenty-five male Wistar albino mice were divided into the following five groups. The CON-group received normal saline; the Pb-group received PbAc; the Pb + Q-CRN group received lead acetate followed by quercetin; the Q-CRN group received quercetin; and the CRN group received corn oil. After 4 weeks, the mice were euthanized. It was speculated that Pb significantly increased the levels of serine, threonine, and asparagine and decreased the levels of valine, lysine, and glutamic acid in the plasma of Pb-group, thus impairing amino acid metabolism. However, in the Pb + Q-CRN group, the level of these six amino acids was restored significantly due to the ameliorative effect of quercetin. The presence of lipid metabolites (L-carnitine, sphinganine, phytosphingosine, and lysophosphatidylcholine) in mice serum was confirmed by ESI/MS. The GPx, SOD, GSH, and CAT levels were significantly decreased, and the MDA level was significantly increased, thus confirming the oxidative stress and lipid peroxidation in the Pb group. The antioxidant effect of quercetin was elucidated in the Pb + Q-CRN group. Expression of CPT-I, CPT-II, LCAT, CROT, CACT, and MTR genes was significantly upregulated in the liver of Pb goup mice. Hence, the findings of this study proved that Pb exposure induced oxidative stress, upregulated gene expression, and impaired the lipid and amino acid metabolism in mice.
Differential subsampling with cartesian ordering: A high spatial-temporal resolution dixon imaging sequence for assessment of dural arteriovenous fistula
Authors: Chen Xi , Ge Liang , Wan Hailinlin , Huang Lei , Jiang Yeqing , Lu Gang , Wang Jing , Zhang Xiaolong
Published: October 2022

Objective

To evaluate the accuracy of differential subsampling with cartesian ordering (DISCO) in comparison to time of flight (TOF) in detecting dural arteriovenous fistulas (DAVF), cerebral venous thrombosis (CVT) and hemodynamics.

Methods

Sixty-two cases (24 female; aged 14–75; mean age, 51.3 years) were included in our study, with 42 positive and 20 negative cases via Digital Subtraction Angiography (DSA). Two neuroradiologists independently evaluated the DISCO and TOF. The sensitivity, specificity, and accuracy of the DISCO and TOF-MRA were individually calculated using DSA as the gold standard. Inter-observer reliability was assessed by using a weighted Cohen's kappa (κ) test; P < 0.05 was set as the threshold for statistical significance.

Results

Diagnostic sensitivities of DISCO and TOF for DAVF were 92.86 and 64.29%; specificities were 95.0% and 95.0%; while accuracies were 93.55 and 74.19% respectively. For detected CVT, sensitivities of DISCO and TOF were 100 and 92.31%; specificities were 96.55 and 93.10%; with accuracies 97.62 and 92.86% respectively. In hemodynamic analysis, sensitivity of DISCO for reflux was 95.45%; with a specificity of 95.0%; and accuracy 95.24%. The inter-observer kappa values were 0.857 for DISCO (P < 0.001).

Conclusion

DISCO showed a high degree of sensitivity and specificity, suggesting its effectiveness in detecting DAVF with or without CVT. Intracranial hemodynamics can be identified using DISCO in DAVF patients, providing accurate evaluation of cerebral blood flow dynamics during the pre-treatment phase.
Longitudinal evaluation of olfactory function in individuals with Gaucher disease and GBA1 mutation carriers with and without Parkinson's disease
Authors: Lopez Grisel J. , Lichtenberg Jens , Tayebi Nahid , Ryan Emory , Lecker Abigail L. , Sidransky Ellen
Published: October 2022

Objective

Biallelic mutations in GBA1, which encodes the lysosomal enzyme glucocerebrosidase, cause the lysosomal storage disorder Gaucher disease (GD). In addition, mutations in GBA1 are the most common genetic risk factor for future development of Parkinson's disease (PD). However, most mutation carriers will never develop parkinsonism. Olfactory dysfunction is often a prodromal symptom in patients with PD, appearing many years prior to motor dysfunction. The purpose of this study was to assess olfactory function longitudinally in individuals with and without parkinsonism who carry at least one GBA1 mutation.

Methods

One hundred seventeen individuals who participated in a natural history study of GD at the National Institutes of Health were evaluated using the University of Pennsylvania Smell Identification Test (UPSIT) during a 16-year period. Seventy patients with GD (13 with PD) and 47 GBA1 carriers (9 with PD) were included. Fifty-six of the total (47.9%) were seen over multiple visits, and had UPSIT screening performed two to six times, with time intervals between testing ranging from 2 to 6 years. Comparative and control data were obtained from the Parkinson's Progression Markers Initiative (PPMI) database (519 individuals, including 340 with idiopathic PD and 179 healthy controls). Statistical analysis was performed using R.

Results

Severe hyposmia and anosmia was evident in both GBA1 heterozygotes and homozygotes with PD. 84% without parkinsonism had UPSIT scores >30, and those who underwent repeated testing maintained olfactory function over time. No statistically significant difference in UPSIT scores was found between mutation carriers with and without a family history of parkinsonism. A small group of individuals without PD scored in the moderate-severe microsmia range. No significant differences in olfaction were found among our GBA1-PD cohort and idiopathic PD cohort obtained from PPMI.