bluuebunny/crossref_metadata_2025 huggingface dataset:
ChemInform Abstract: An Expeditious Route to Streptococci and Enterococci Glycolipids via Ring‐Opening of 1,2‐Anhydrosugars with Protic Acids.
Authors: TIMMERS C. M. , VAN STRATEN N. C. R. , VAN DER MAREL G. A. , VAN BOOM J. H.
Published: June 2010

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Characterization of Chemical Constituents of Gliricidia meculata Leaves
Authors: ALI M. , SHARMA N.
Published: June 2010

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: A New Dimeric Coumarin from Boenninghausenia albiflora Reichb and Meissner (Rutaceae).
Authors: BANERJI J. , SAHA M. , MUKHERJEE P. , MONDAL S. , JOSHI P. C.
Published: June 2010

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Three Isoflavanones from Erythrina orientalis.
Authors: TANAKA H. , TANAKA T. , HOSOYA A. , KITADE Y. , ETOH H.
Published: June 2010

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: A Pterocarpan from Ulex parviflorus.
Authors: MAXIMO P. , LOURENCO A.
Published: June 2010

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Isochroman Structures in Lignin: A New β‐1 Pathway.
Authors: RALPH J. , PENG J. , LU F.
Published: June 2010

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Alternative Synthesis of (‐)‐Malyngolide Utilizing (‐)‐Quinic Acid.
Authors: MATSUO K. , MATSUMOTO T. , NISHIAKI K.
Published: June 2010

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Cytotoxic Metabolites Produced by a Fungal Strain from a Sargassum Alga.
Authors: AMAGATA T. , MINOURA K. , NUMATA A.
Published: June 2010

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Bent Metallocenes Revisited
Authors: GREEN J. C.
Published: June 2010

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Carbocycles from Carbohydrates via Free Radical Cyclizations: Synthesis and Manipulation of Annulated Furanoses
Authors: MARCO‐CONTELLES J. , ALHAMBRA C. , MARTINEZ‐GRAU A.
Published: June 2010

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: C‐Glycosidation Technology with Free Radical Reactions
Authors: TOGO H. , HE W. , WAKI Y. , YOKOYAMA M.
Published: June 2010

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Biomimetic Formation and Interconversion in the Heteroyohimbine Series
Authors: LOUNASMAA M. , HANHINEN P.
Published: June 2010

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Forming Cyclics with the Intramolecular Heck Reaction
Authors: LINK J. T. , OVERMAN L. E.
Published: June 2010

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: The First Enantioselective Syntheses of Vicinal Difluoropyrrolidines and the First Catalytic Asymmetric Synthesis Mediated by the C2 Symmetry of a ‐CHFCHF‐ Unit.
Authors: MARSON C. M. , MELLING R. C.
Published: June 2010

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Camphor‐Derived 1,4‐Oxathianes for Carbonyl Epoxidation.
Authors: AGGARWAL V. K. , FORD J. G. , JONES R. V. H. , FIELDHOUSE R.
Published: June 2010

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Transition‐Metal‐Mediated Dihydropyran Syntheses. exo Selective Hetero Diels—Alder Reactions of Cobaloxime Dienyl Complexes with Aldehydes.
Authors: ADAMS T. A. , WELKER M. E. , DAY C. S.
Published: June 2010

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Synthesis of Thiophene‐Substituted 3H‐Naphtho[2,1‐b]pyrans, Precursors of Photomodulated Materials.
Authors: MOUSTROU C. , REBIERE N. , SAMAT A. , GUGLIELMETTI R. , YASSAR A. E. , DUBEST R. , AUBARD J.
Published: June 2010

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Substituted Xanthones as Antimycobacterial Agents. Part 1. Synthesis and Assignment of 1H/13C NMR Chemical Shifts.
Authors: PICKERT M. , FRAHM A. W.
Published: June 2010

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Synthesis of 8‐Quinolinyl Ethers under Microwave Irradiation.
Authors: WANG J.‐X. , ZHANG M. , HU Y.
Published: June 2010

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Antitumor Polycyclic Acridines. Part 5. Synthesis of 7H‐Pyrido[4,3,2‐kl]acridines with Exploitable Functionality in the Pyridine Ring.
Authors: JULINO M. , STEVENS M. F. G.
Published: June 2010

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Reaction of (α‐Halogenoalkyl)thiiranes with Nucleophilic Reagents. Part 2. Reaction of (α‐Chloroalkyl)thiiranes and Epihalogenohydrins with N,N′‐Dimethylethylenediamine.
Authors: TOMASHEVSKII A. A. , SOKOLOV V. V. , POTEKHIN A. A.
Published: June 2010

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Relationship Between Dihedral Angles of N1 and C9 Substituents in 1,4‐Benzodiazepines and Dual Cholecystokinin‐A and ‐B Antagonistic Activities.
Authors: TABUCHI S. , NAKANISHI I. , SATOH Y.
Published: June 2010

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Adaptation of the Barton Reaction to Carborane Chemistry: The Synthesis and Reactivity of 2‐Hydroxyimino‐1‐hydroxymethyl‐nona‐B‐methyl‐1,12‐dicarba‐closo‐dodecabo rane(12).
Authors: HERZOG A. , KNOBLER C. B. , HAWTHORNE M. F.
Published: June 2010

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Synthesis of Novel Thio(seleno) Phosphate‐phosphonates and Their Biological Activities.
Authors: ZHOU J. , CHEN R. , YANG X.
Published: June 2010

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: On Steroids. Part 396. N‐Benzoyl‐N‐methylandrostan‐17β‐amines; 20‐Aza Analogues of Brassinolide.
Authors: SLAVIKOVA B. , KASAL A. , KOHOUT L.
Published: June 2010

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Synthesis of Silicon‐Containing Alanines.
Authors: SIBI M. P. , HARRIS B. J. , SHAY J. J. , HAJRA S.
Published: June 2010

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Rapid Synthesis of Matrix Metalloproteinase Inhibitors via Ugi Four‐Component Condensation.
Authors: FLOYD C. D. , HARNETT L. A. , MILLER A. , PATEL S. , SAROGLOU L. , WHITTAKER M.
Published: June 2010

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Towards an Asymmetric Synthesis of ADDA Conjugates.
Authors: CUNDY D. J. , DONOHUE A. C. , MCCARTHY T. D.
Published: June 2010

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: A Novel Method for Synthesizing Aldonophenylhydrazono‐Lactones.
Authors: WANG J. , WANG L. , MENG X. , ZHANG P.
Published: June 2010

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: An Epoxide Derived from D‐Glucose as the Key Intermediate for Penaresidine and Sphingolipids Synthesis.
Authors: BEAUHAIRE J. , DUCROT P.‐H.
Published: June 2010

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Glycosylimidates. Part 79. Synthesis of a Thio‐Linked Lewis A (Lea) Epitope.
Authors: EISELE T. , WINDMUELLER R. , SCHMIDT R. R.
Published: June 2010

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Structural Studies on the Lipopolysaccharide from a Rough Strain of Ochrobactrum anthropi Containing a 2,3‐Diamino‐2,3‐dideoxy‐D‐glucose Disaccharide Lipid A Backbone.
Authors: VELASCO J. , MOLL H. , KNIREL Y. A. , SINNWELL V. , MORIYON I. , ZAEHRINGER U.
Published: June 2010

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Structural Characterization of the Galactoxylomannan of Cryptococcus neoformans Cap67.
Authors: VAISHNAV V. V. , BACON B. E. , O'NEILL M. , CHERNIAK R.
Published: June 2010

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Synthetic Studies on Sialoglycoconjugates. Part 106. Synthesis of Sialyl Lex Ganglioside Analogues Modified at C‐6 of the Galactose Residue to Elucidate the Mechanism of Selection Recognition.
Authors: OTSUBO N. , ISHIDA H. , KISO M. , HASEGAWA A.
Published: June 2010

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Enantioselective Synthesis of the Quinolizidine Alkaloids (+)‐Myrtine and (‐)‐Epimyrtine.
Authors: GARDETTE D. , GELAS‐MIALHE Y. , GRAMAIN J.‐C. , PERRIN B. , REMUSON R.
Published: June 2010

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: A Simple Regioselective Synthesis of (R)‐10‐Hydroxyaporphine Directly from (R)‐10,11‐Dihydroxyaporphine [(R)‐Apomorphine].
Authors: KIM J. C. , BAE S.‐D. , KIM J.‐A , CHOI S.‐K.
Published: June 2010

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Synthesis of Nucleosides of Pyrido[2,3‐d]pyrimidines and Their Microbial Activity.
Authors: SINGH G. , SWATI SWATI , MISHRA A. K. , PRAKASH L.
Published: June 2010

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Design, Synthesis, and Self‐Assembly of “Artificial Dinucleotide Duplexes”.
Authors: SESSLER J. L. , WANG R.
Published: June 2010

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Synthesis of New Building Blocks: Towards the Analogues of Peptide Nucleic Acids (PNAs).
Authors: DALLAIRE C. , ARYA P.
Published: June 2010

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Pharmacologically Active Components of Viticis Fructus (Vitex rotundifolia). Part 2. The Components Having Analgesic Effects.
Authors: OKUYAMA E. , FUJIMORI S. , YAMAZAKI M. , DEYAMA T.
Published: June 2010

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: The New Cubic Iron‐Nitride Phase FeN Prepared by Reactive Magnetron Sputtering.
Authors: RISSANEN L. , NEUBAUER M. , LIEB K. P. , SCHAAF P.
Published: June 2010

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: NMR and NQR Studies of Borate Glasses
Authors: BRAY P. J. , PETERSEN G. L.
Published: June 2010

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Breeding Material Database for Fusion Reactor. Part 3. Li2TiO3 Solid Breeder
Authors: FUTAMURA Y. , KAWAMURA H. , TSUCHIYA K.
Published: June 2010

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Status of International Research in the Field of Fullerenes
Authors: KUZMANY H.
Published: June 2010

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Experimental and Numerical Studies of Oxidation Chemistry and Spontaneous Ignition Phenomena
Authors: GRIFFITHS J. F. , MOHAMED C.
Published: June 2010

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Applications of Nuclear Quadrupole Interactions in Materials Research
Authors: ZHU S. Y.
Published: June 2010

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Interpersonal contribution to outcome: the relation of interpersonal distress and symptomatic improvement as a result of psychotherapy
Authors: Hardy Amanda O. , Tracey Terence J. G. , Glidden‐Tracey Cynthia , Hess Timothy R. , Rohlfing Jessica E.
Published: June 2010

The study utilized a 32‐item brief measure of interpersonal problems (IIP) to examine interpersonal distress in relation to symptomology and treatment outcome as assessed by the Outcome Questionnaire‐45 (OQ‐45). The study included a community sample of 210 individuals receiving psychotherapy at a mental health training facility. A number of significant correlations were found between interpersonal distresses with symptomology. The highest correlations were found between levels of general interpersonal distress and symptoms relating to psychiatric and social distress at the onset of therapy; in general the IIP did not differentiate between symptomology types. The study failed to replicate previous research indicating a relation between facets of interpersonal problems with treatment outcome. Copyright © 2010 John Wiley & Sons, Ltd.

Key Practitioner Message:

• Evidence is provided for the use of a brief measure of interpersonal problems (IIP‐32) as a valid alternative to lengthier versions in a clinical setting.

• Interpersonal problems are associated with various facets of clinical symptomology but do little to differentiate between symptomology types.

• Reported rates of general interpersonal distress at the onset of therapy differ among clients that do and do not make clinically significant changes in areas of overall symptomology and interpersonal difficulties.
Oxygenated Stereotriads with Definite Absolute Configuration by Lipase‐Mediated Kinetic Resolution: De Novo Synthesis of Imino Sugars and 6‐Deoxy‐C‐glycosides
Authors: Acetti Daniela , Brenna Elisabetta , Gatti Francesco G.
Published: June 2010

The lipase‐mediated kinetic resolution of compound 1 was investigated to prepare chiral synthons showing oxygenated stereotriads in a definite configurational arrangement. These key intermediates were embedded in biologically relevant structures, such as hydroxylated pyrrolidine 3 and C‐phenyl glycosides by classical organic reactions.
A simplified pricing model for volatility futures
Authors: Dupoyet Brice , Daigler Robert T. , Chen Zhiyao
Published: June 2010

We develop a general model to price VIX futures contracts. The model is adapted to test both the constant elasticity of variance (CEV) and the Cox–Ingersoll–Ross formulations, with and without jumps. Empirical tests on VIX futures prices provide out‐of‐sample estimates within 2% of the actual futures price for almost all futures maturities. We show that although jumps are present in the data, the models with jumps do not typically outperform the others; in particular, we demonstrate the important benefits of the CEV feature in pricing futures contracts. We conclude by examining errors in the model relative to the VIX characteristics. © 2010 Wiley Periodicals, Inc. Jrl Fut Mark 31:307–339, 2011
Evaluating the possible genotoxic, mutagenic and tumor cell proliferation‐inhibition effects of a non‐anticoagulant, but antithrombotic algal heterofucan
Authors: Almeida‐Lima Jailma , Costa Leandro Silva , Silva Naisandra Bezerra , Melo‐Silveira Raniere Fagundes , Silva Fábio Vasconcelos , Cansanção Felipe Maria Beatriz Mesquita , Batistuzzo Medeiros Silvia Regina , Leite Edda Lisboa , Rocha Hugo Alexandre Oliveira
Published: June 2010

Fucan is a term used to denominate a family of sulfated polysaccharides rich in L‐fucose. They are extracted mainly from brown seaweeds and echinoderms. The brown seaweed Spatoglossum schröederi (Dictyotaceae) synthesizes three heterofucans named A, B and C. Our research group purified a non‐anticoagulant heterofucan (fucan A) which displays antithrombotic activity in vivo. However, its in vitro toxicity has yet to be determined. This work presents the evaluation of the potential cytotoxicity, mutagenicity and genotoxicity of this fucan. After 48 h incubation fucan A cytotoxicity was determinate using MTT assay. Tumor‐cell (HeLa, PC3, PANC, HL60) proliferation was inhibited 2.0–43.7%; at 0.05–1 mg ml−1 of the heterofucan, the 3T3 non‐tumor cell line proliferation was also inhibited (3.3–22.0%). On the other hand, the CHO tumorigenic and RAW non‐tumor cell lines proliferation were not affected by this molecule (0.05–1 mg ml−1). We observed no mutagenic activity in Salmonella reversion assay when bacterial strains TA97a, TA98, TA100 and TA102 (with and without S9) were used. Comet assay showed that fucan A had no genotoxic effect (from 20 to 1000 µg ml−1) on CHO cells. In conclusion, this study indicates that the S. schröederi fucan A was not found to be genotoxic or mutagenic compound; thus it could be used in new antithrombotic drug development. Copyright © 2010 John Wiley & Sons, Ltd.
Separation of flavonoid and alkaloid using collagen fiber adsorbent
Authors: Li Juan , Liao Xuepin , Liao Guanhao , He Qiang , Zhang Wenhua , Shi Bi
Published: June 2010

Flavonoids and alkaloids are two major classes of plant compounds with biological activities, and they usually coexist in many medicinal herbs. In this study, a novel collagen fiber adsorbent (CFA) was prepared, and its application for adsorption chromatography separation of flavonoids and alkaloids was systematically investigated. The typical flavonoids, rutin and baicalin, and the typical alkaloids, matrine and caffeine, were selected as probe molecules for the investigations. The batch adsorption behaviors of these compounds on CFA in different solvents indicated that hydrogen bond plays a predominant role for the adsorption of flavonoid and alkaloid in pure ethanol, while the hydrophobic interaction plays a predominant role for the adsorption in water. In column chromatography separation, flavonoids were completely separated from alkaloids by a stepwise elution process with pure ethanol followed by aqueous ethanol solution. The two flavonoids, rutin and baicalin, were also well separated although the two alkaloids, matrine and caffeine, were washed out together. The optimal loading volume of sample solution (10 mg/mL) for the separation was determined as 0.66 mL/g CFA. Under these conditions, flavonoid and alkaloid were effectively separated with a recovery higher than 90% in 8 times repeated applications.
A simple methodology for the determination of fatty acid composition in edible oils through 1H NMR spectroscopy
Authors: Barison Andersson , Pereira da Silva Caroline Werner , Campos Francinete Ramos , Simonelli Fabio , Lenz Cesar Antonio , Ferreira Antonio Gilberto
Published: June 2010

A simple methodology for the determination of the fatty acid composition of edible oils through 1H NMR is proposed. The method is based on the fact that all fatty acid chains are esterified to a common moiety, glycerol, and the quantification is done directly in the 1H NMR spectra through the relationship between the areas of a characteristic signal of each fatty acid and a signal of the glycerol moiety, without the use of mathematical equations. The methodology was successfully applied to determine the fatty acid composition of several edible oils, with equivalent results to those given by the AOAC Official method by gas chromatography. Its main advantages are simplicity and the lack of need for sample pre‐treatment such as derivatization or extraction. Copyright © 2010 John Wiley & Sons, Ltd.
Intraoperative on‐nerve nerve conduction study and conversion factor in the sural nerve
Authors: Oh Shin J. , Hemmi Shoji , Kurokawa Katsumi , Hatanaka Yuki
Published: June 2010

To determine the conversion factor (CF) of the sural nerve the correlation between the maximum nerve conduction velocity (NCV) and the diameter of the largest fibers was studied in 30 patients suspected of having neuropathy. Sensory nerve action potentials were obtained by on‐nerve needle nerve conduction study using needle electrodes placed on the exposed sural nerve during biopsy. The CF was 4.3 (n = 2) in normal sural nerves and close to the normal value (3.85, n = 4) in axonal neuropathy. The CF in demyelinating neuropathy was smaller than the normal value (2.77, n = 24), indicating disproportionately slower conduction than expected from the diameter of nerve fibers. The CF was helpful in differentiating between demyelinating and axonal neuropathies. We propose that a 36% decrease from the mean value of NCV is a reasonable criterion for demyelination of the nerve. Muscle Nerve, 2010
Biochemie und Molekularbiologie 2007
Authors: Marx Andreas , Hartig Jörg S.
Published: July 2010

Modifizierte, künstliche Systeme der Replikation und der Regulation der Expression leisten wertvolle Beiträge zur synthetischen Biologie. Zum Proteindesign lassen sich evolutive und rationale Methoden verwenden, zunehmend auch kombinierte Ansätze, welche die Vorteile beider Methoden vereinen. Durchbruch bei der Stammzellforschung: Induzierte pluripotente Stammzellen könnten den Weg in Richtung Stammzelltherapie weisen.
Chemiedidaktik 2007
Authors: Becker Hans‐Jürgen , Stäudel Lutz
Published: July 2010

Bildungsstandards sind die Messpunkte chemiebezogener Kompetenzen von Schülern. Noch beruhen die Kompetenzmodelle, die Fixpunkte künftiger Unterrichtsprogramme sind, auf Hypothesen. Kompetenzorientierung soll leistungssteigernd wirken und den Chemieunterricht insgesamt verbessern. Diese Ausrichtung der chemischen Bildungsdiskussion ist zwar visionär, im Kern aber nicht unbekannt.
Technische Chemie 2007
Authors: Brüggemann Oliver , Busch Markus , Wolf Dorit
Published: July 2010

Neue, verbesserte Katalysatoren und effizientere Prozesse entscheiden über den Erfolg von Techniken wie Fischer‐Tropsch‐Synthese und Methanol‐Downstream‐Verfahren zur CO2‐Reduktion. Weiterhin forscht die technische Chemie zunehmend für innovative Materialien und deren Herstellung.
Reach‐Fäden laufen in Helsinki zusammen
Authors: Osterath Brigitte
Published: July 2010

Die Europäische Agentur für chemische Stoffe hat ihre Büros im Stadtzentrum von Helsinki bezogen. In den nächsten zehn Jahren werden etwa 30 000 chemische Stoffe registriert ‒ im Juni beginnt die Vorregistrierungsphase.
“Wir müssen die Dynamik in aufstrebenden Märkten nutzen”
Authors: Guggolz Ernst , Hübel Marcus
Published: July 2010

Die westliche Chemieindustrie muss sich in Volkswirtschaften wie China oder Indien behaupten. Wie dabei grundsätzlich Vorteile zu nutzen und Gefahren zu umgehen sind, beschreibt Marcus Hübel, Chemiker bei der Unternehmensberatung Accenture und Experte für Strategie und Marketing der chemischen Industrie in China.
Zellsekretanalytik
Authors: Peter Jochen , Otto Angela , Wolf Bernhard
Published: July 2010

Die Probenvorbereitung mit superparamagnetischen Mikropartikeln steigert die Nachweisempfindlichkeit der chromatographischen Trennung und Detektion von sekretierten Peptiden aus Zellkulturen.
Jahrestreffen der Senioren – ein Erfolg
Authors: Bengs Holger
Published: July 2010

Über 70 Teilnehmer beim ersten Jahrestreffen der Seniorexperten Chemie: Interessante Vorträge und intensive Diskussionen gepaart mit Wiedersehensfreude. Drei belebende Konferenztage für Jung und Alt. Gibt es eine Fortsetzung?
Der Weg zu international vergleichbaren Messergebnissen
Authors: Schiel Detlef , Güttler Bernd , Rienitz Olaf , Maschat Ralf , Koch Michael , Borchers Ulrich
Published: July 2010

Richtlinien und Normen fordern von der chemischen Analytik zunehmend europaweit vergleichbare Messergebnisse. Als Bezugspunkte eignen sich die Konsenswerte regionaler Vergleichsmessungen kaum. Dagegen lässt sich mit metrologischen Infrastrukturen weltweite Vergleichbarkeit und Richtigkeit von Messergebnissen erreichen.
“Viele sehr gute, auch exzellente”
Authors: Diederich Francois , Guggolz Ernst
Published: July 2010

Welche Überlegungen hinter dem Forschungsrating Chemie stecken, welchen Wert es hat und welche Konsequenzen zu ziehen sind, kommentiert der Leiter der Bewertungsgruppe, ETH‐Professor François Diederich.
SusChem – Berlin points to future priorities
Authors: Mours Marian , Townsend Rodney
Published: July 2010

The European Technology Platform (ETP) for Sustainable Chemistry (SusChem) is of key importance to all involved in molecular science in Europe. SusChem is addressing head‐on the challenge of developing sustainable technologies, based on chemistry, chemical engineering and industrial biotechnology that will deliver future prosperity and health for everyone.
Low anthracyclines doses‐induced cardiotoxicity in acute lymphoblastic leukemia long‐term female survivors
Authors: Amigoni Maria , Giannattasio Cristina , Fraschini Donatella , Galbiati Marianna , Capra Anna Clara Maria , Madotto Fabiana , Cesana Francesca , Jankovic Momcilo , Masera Giuseppe , Mancia Giuseppe
Published: June 2010

Background

High dosage anthracyclines in pediatric patients with acute lymphoblastic leukemia (ALL) is associated with cardiotoxicity. However, data on the cardiac effects of lower cumulative doses of these drugs are not conclusive. The aim of this study was to assess the cardiac effects of low cumulative anthracycline doses in long‐term survivors of ALL.

Procedure

Echocardiograms were performed on 62 long‐term ALL survivors, without any overt or sub‐clinical signs or symptoms of heart failure. The interval after stopping therapy was 12.6 ± 4.3 years; the mean cumulative dose of anthracyclines was 228.2 ± 42.3 mg/m2. Left ventricular (LV) structure and function were studied by echocolor‐Doppler. An age, gender and body surface area (BSA) matched group of healthy subjects was used as controls. Cardiac data were analyzed before and after BSA normalization.

Results

Long term survivors of ALL, showed a lower LV mass index, interventricular septal and posterior wall thickness, which were independently related to gender and to age at which the ALL diagnosis was made. Data analyzed according to gender showed that abnormalities were confined to the female group. No alterations were observed in the ALL male group versus the corresponding control group. No relationship was observed between the echocardiografic abnormalities and the duration of follow‐up or the anthracycline mean dose employed.

Conclusions

In the absence of any signs or symptoms of heart failure, female ALL survivors treated with low cumulative anthracycline doses, showed a reduced LV mass and wall thickness. This suggests that in female ALL survivors an echocardyographic follow‐up should be recommended. Pediatr Blood Cancer. 2010;55:1343–1347. © 2010 Wiley‐Liss, Inc.
Direct evidence of a heterotrimeric complex of human interleukin‐4 with its receptors
Authors: Hoffman Ross C. , Castner Beverly J. , Gerhart Mary , Gibson Marylou G. , Rasmussen Brian D. , March Carl J. , Weatherbee James , Tsang Monica , Gustchina Alla , Schalk‐Hihi Céline , Reshetnikova Ludmila , Wlodawer Alexander
Published: June 2010

The mode of binding of interleukin‐4 (IL‐4) to its two known receptors, specific receptor IL‐4R and a shared receptor γc, was investigated using gel filtration and gel electrophoresis. A ternary complex between IL‐4 and the soluble domains of the two receptors was shown to exist in solution. The association constant between γc and the stable complex of IL‐4/sIL‐4R is in the millimolar range, making the ternary complex a feasible target for crystallization studies.
Structures of partridge egg‐white lysozyme with and without tri‐N‐acetylchitotriose inhibitor at 1.9 Å resolution
Authors: Turner M. A. , Howell P. L.
Published: June 2010

The three‐dimensional structures of native partridge egg‐white lysozyme (PEWL) and PEWL complexed with tri‐N‐acetylchitotriose inhibitor have been determined crystallographically and refined at 1.9 Å resolution. Crystals of native and complexed protein are isomorphous and have space group and cell dimensions that are identical to those of hen egg‐white lysozyme (HEWL) under similar crystallization conditions. Full occupancy of the trisac‐charide in the inhibitor complex has allowed definitive modeling and refinement of all three sugar residues, located at subsites A, B, and C in the PEWL active site. A comparison has been made with HEWL/inhibitor complexes in which coordinates were either not refined (Blake CCF, et al., 1967, Proc R Soc B 167:378–388) or were refined at partial occupancy (Cheetham JC, Artymiuk PJ, Phillips DC, 1992, J Mol Biol 224:613–628). Although the loop comprising residues 70–75 is located on the surface of the protein and not near the active site, it appears to be affected indirectly by trisaccharide binding such that the loop shifts toward the active site and becomes relatively immobilized. The source of this loop movement appears to be the anchoring of Trp 62, located in the active site cleft, as it forms a hydrogen bond with O6 of the N‐acetylglucosamine at site C. Good electron density for the trisaccharide in the PEWL complex structure shows that Asp 101 is involved in hydrogen bonding interactions with the terminal sugar residue.
Homology model of human interferon‐α8 and its receptor complex
Authors: Seto Marian H. , Harkins Richard N. , Adler Marc , Whitlow Marc , Croze Ed , Bret Church W.
Published: June 2010

Human interferon‐α8 (HuIFNα8), a type I interferon (IFN), is a cytokine belonging to the hematopoietic super‐family that includes human growth hormone (HGH). Recent data identified two human type I IFN receptor components. One component (p40) was purified from human urine by its ability to bind to immobilized type I IFN. A second receptor component (IFNAR), consisting of two cytokine receptor‐like domains (D200 and D200′), was identified by expression cloning. Murine cells transfected with a gene encoding this protein were able to produce an antiviral response to human IFNα8. Both of these receptor proteins have been identified as members of the immunoglobulin superfamily of which HGH receptor is a member. The cytokine receptor‐like structural motifs present in p40 and IFNAR were modeled based on the HGH receptor X‐ray structure. Models of the complexes of HuIFNα8 with the receptor subunits were built by superpositioning the conserved Cα backbone of the HuIFNα8 and receptor subunit models with HGH and its receptor complex. The HuIFNα8 model was constructed from the Cα coordinates of murine interferon‐β crystal structure. Electrostatic potentials and hydrophobic interactions appear to favor the model of HuIFNα8 interacting with p40 at site 1 and the D200′ domain of IFNAR at site 2 because there are regions of complementary electrostatic potential and hydrophobic interactions at both of the proposed binding interfaces. Some of the predicted receptor binding residues within HuIFNα8 correspond to functionally important residues determined previously for human IFNα1, IFNα2, and IFNα4 subtypes by site‐directed mutagenesis studies. The models predict regions of interaction between HuIFNα8 and each of the receptor proteins, and provide insights into interactions between other type I IFNs (IFN‐α subtypes and IFN‐β) and their respective receptor components.
Anti‐peptide monoclonal antibody imaging of a common binding domain involved in muscle regulation
Authors: Van Eyk Jennifer E. , Caday‐Malcolm Rosalinda A. , Hodges Robert S. , Yu Lei , Irvin Randall T.
Published: June 2010

Multiple‐component regulatory protein systems function through a generalized mechanism where a single regulatory protein or ligand binds to a variety of receptors to modulate specific functions in a physiologically sensitive context. Muscle contraction is regulated by the interaction of actin with troponin I (TnI) or myosin in a Ca2+‐sensitive manner. Actin utilizes a single binding domain (residues 1–28) to bind to residues 104–115 of TnI (Van Eyk JE, Sönnichsen FD, Sykes BD, Hodges RS, 1991, In: Rüegg JC, ed, Peptides as probes in muscle research, Heidelberg, Germany: Springer‐Verlag, pp 15–31) and to myosin subfragment 1 (S1, an enzymatic fragment of myosin containing both the actin and ATP binding sites) (Van Eyk JE, Hodges RS, 1991, Biochemistry 30:11676–11682) in a Ca2+‐sensitive manner. We have utilized an anti‐TnI peptide (104–115) monoclonal antibody, Mab B4, that binds specifically to TnI, to image the common binding domain of actin and thus mimic the activity of actin including activation of the S1 ATPase activity and TnI‐mediated regulation of the S1 ATPase. Mab B4 has also been utilized to identify a receptor binding domain on myosin (residues 633–644) that is recognized by actin. Interestingly, Mab B4 binds to the native protein receptors TnI and S1 with relative affinities of 100‐ and 25,000‐fold higher than the binding affinity to the 12‐residue peptide immunogen. Thus, anti‐peptide monoclonal antibodies prepared against a receptor binding domain can mimic the ligand binding domain and be utilized as a powerful tool for the detailed analysis of complex multiple‐component regulatory systems.
Circular permutation within the coenzyme binding domain of the tetrameric glyceraldehyde‐3‐phosphate dehydrogenase from Bacillus stearothermophilus
Authors: Vignais Marie‐Luce , Corbier Catherine , Mulliert Guillermo , Branlant Christiane , Branlant Guy
Published: June 2010

A circularly permuted (cp) variant of the phosphorylating NAD‐dependent glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH) from Bacillus stearothermophilus has been constructed with N‐ and C‐termini created within the coenzyme binding domain. The cp variant has a kcat value equal to 40% of the wild‐type value, whereas Km and KD values for NAD show a threefold decrease compared to wild type. These results indicate that the folding process and the conformational changes that accompany NAD binding during the catalytic event occur efficiently in the permuted variant and that NAD binding is tighter. Reversible denaturation experiments show that the stability of the variant is only reduced by 0.7 kcal/mol compared to the wild‐type enzyme. These experiments confirm and extend results obtained recently on other permuted proteins. For multimeric proteins, such as GAPDH, which harbor subunits with two structural domains, the natural location of the N‐ and C‐termini is not a prerequisite for optimal folding and biological activity.
Building proteins from Cα coordinates using the dihedral probability grid Monte Carlo method
Authors: Mathiowetz Alan M. , Goddard William A.
Published: June 2010

Dihedral probability grid Monte Carlo (DPG‐MC) is a general‐purpose method of conformational sampling that can be applied to many problems in peptide and protein modeling. Here we present the DPG‐MC method and apply it to predicting complete protein structures from Cα coordinates. This is useful in such endeavors as homology modeling, protein structure prediction from lattice simulations, or fitting protein structures to X‐ray crystallographic data. It also serves as an example of how DPG‐MC can be applied to systems with geometric constraints. The conformational propensities for individual residues are used to guide conformational searches as the protein is built from the amino‐terminus to the carboxyl‐terminus. Results for a number of proteins show that both the backbone and side chain can be accurately modeled using DPG‐MC. Backbone atoms are generally predicted with RMS errors of about 0.5 Å (compared to X‐ray crystal structure coordinates) and all atoms are predicted to an RMS error of 1.7 Å or better.
Similarity of different β‐strands flanked in loops by glycines and prolines from distinct (α/β)8‐barrel enzymes: Chance or a homology?
Authors: Janeček ŠTefan
Published: June 2010

Many (α/β)8‐barrel enzymes contain their conserved sequence regions at or around the β‐strand segments that are often preceded and succeeded by glycines and prolines, respectively. α‐Amylase is one of these enzymes. Its sequences exhibit a very low degree of similarity, but strong conservation is seen around its β‐strands. These conserved regions were used in the search for similarities with β‐strands of other (α/β)8‐barrel enzymes. The analysis revealed an interesting similarity between the segment around the β2‐strand of α‐amylase and the one around the β4‐strand of glycolate oxidase that are flanked in loops by glycines and prolines. The similarity can be further extended on other members of the α‐amylase and glycolate oxidase subfamilies, i.e., cyclodextrin glycosyltransferase and oligo‐l,6‐glucosidase, and flavocytochrome b2, respectively. Moreover, the α‐subunit of tryptophan synthase, the (α/β)8‐barrel enzyme belonging to the other subfamily of (α/β)8‐barrels, has both investigated strands, β2 and β4, similar to β2 of α‐amylase and β4 of glycolate oxidase. The possibilities of whether this similarity exists only by chance or is a consequence of some processes during the evolution of (α/β)8‐barrel proteins are briefly discussed.