bluuebunny/crossref_metadata_2025 huggingface dataset:
Crop diversification and income of agricultural households in India: an empirical analysis
Authors: Basantaray A. K. , Acharya Shailesh , Patra Tophan
Published: March 2024

The present study empirically tests the relationship between crop diversification and farm income. The study area is India, the fifth largest economy of the world, and the units of study are its households which dominates India’s labor force. The propensity score matching technique is applied on the two waves of nationally representative data on agricultural households pertaining to agricultural year 2012–13 & 2018–19 to test the relationship between crop diversification and farm income. We find a strong and positive impact of crop diversification on farm income as farm income increases by around 13 per cent if non-diversified households opt for crop diversification. Furthermore, important factors such as literacy, access to market, access to irrigation, agricultural training, farming experience, and household size positively affects crop diversification at the household level. However, recent decline in the extent of diversification in India is a cause for concern. Promotion of cultivation of HVCs by giving them such institutional supports as initial capital required for switch to HVCs and other steps that insulates them from higher risks is the need of the hour in Indian agriculture.
Image and audio characterization of electromagnetic signals in cable defect detection
Authors: Jiang Xueli , Wang Jishuo , Zhao Chaoyang , Yuan Weifeng
Published: March 2024

Power cables play a very important role in urban development. On the other hand, the integrity and the safe operation of power cables are crucial as even a minor potential mishap can result in serious loss of life and property. Hence, early diagnosis and warning of cable faults are imperative. Addressing the current lack of practical detection technologies, this study proposes a non-destructive testing method based on electromagnetic field principles. Through scanning the power-on power cables with probes, the electric field intensity around the cables can be measured, and the weak anomaly caused by structural and material defects can be detected using Superlets transformation. Additionally, to gain a better characterization of the faults, a digital post-processing approach consisting of imaging and sonification algorithms is developed to aid in pinpointing the location of the faults. Both numerical simulation and experimental test indicate that the proposed non-destructive detection method is feasible and can achieve good accuracy in locating cable faults. With image and audio characterization, the present method has great potential applications in ensuring the safety of power cables.
Complex trajectories in higher education students: online and face-to-face universities
Authors: Villar-Aguilés Alícia , Muñoz-Rodríguez David
Published: March 2024

Expansion in higher education and changes in student profiles have led to an increase in non-linear trajectories that do not fit into a time frame considered standard. However, universities continue to establish success and failure parameters relating to performance indicators that do not consider the heterogeneity of trajectories. The theoretical perspective on which we base our work is the complexity approach, which resituates what we understand as a trajectory of success (and failure), incorporating a broader view to better understand how students navigate through higher education. Based on research on university dropout and changes of trajectory, we present the results of a study carried out using sequence analysis in face-to-face and online universities. The research question on which this study focuses is whether, given the context of greater heterogeneity and complexity, there could be a greater similarity between the students’ trajectories in face-to-face and online modalities in relation to complex trajectories. The results show that complex trajectories are very present in both modalities, and differences are observed according to students’ gender and age. A key conclusion is the discussion on how we consider success and failure student trajectories in the contemporary university taking into account the perspective of complexity.
Tycoon candidates, electoral strategies, and voter support: a survey experiment in South Africa
Authors: Justesen Mogens K. , Markus Stanislav
Published: March 2024

Why do voters shun some business tycoons yet elect others into power? As structural conditions facilitate the entry of super-wealthy actors into politics, the differential electoral support across business elites suggests a puzzle. We conceptualize four mechanisms behind the popular support for “tycoon candidates”: competence signaling, framing, fame, and clientelism. To test their relative efficacy, we leverage an experiment embedded in a nationally representative survey in South Africa, an important developing democracy where certain tycoons are successfully running for office. We find that, across distinct electoral appeals by tycoon candidates, clientelism is particularly effective, especially for mobilizing support from the less affluent voters. Racial framing significantly decreases support among white voters. Meanwhile, tycoons’ competence signaling or fame do not help them at the ballot box. By identifying the micro-level underpinnings of voter support across tycoon candidates, our study contributes to the literatures on business and politics, voting behavior, and clientelism.
Polypositroids
Authors: Lam Thomas , Postnikov Alexander
Published: March 2024

We initiate the study of a class of polytopes, which we coin polypositroids, defined to be those polytopes that are simultaneously generalized permutohedra (or polymatroids) and alcoved polytopes. Whereas positroids are the matroids arising from the totally nonnegative Grassmannian, polypositroids are “positive” polymatroids. We parametrize polypositroids using Coxeter necklaces and balanced graphs, and describe the cone of polypositroids by extremal rays and facet inequalities. We introduce a notion of

$(W,c)$

-polypositroid for a finite Weyl group W and a choice of Coxeter element c. We connect the theory of

$(W,c)$

-polypositroids to cluster algebras of finite type and to generalized associahedra. We discuss membranes, which are certain triangulated 2-dimensional surfaces inside polypositroids. Membranes extend the notion of plabic graphs from positroids to polypositroids.
Smallpox Geographies: vaccination, borders and Indigenous peoples in Australia’s coastal north
Authors: Huang Chi Chi , Bashford Alison
Published: March 2024

Australia’s approach to its biosecurity and borders has always been two-pronged – quarantine first, vaccination second. This article asks what this combination looked like in practice by exploring two neglected smallpox vaccination campaigns directed towards Indigenous peoples in the early twentieth century. We argue these were important campaigns because they were the first two pre-emptive, rather than reactionary, vaccination programs directed towards First Nations people. Second, both episodes occurred in Australia’s northern coastline, where the porous maritime geography and proximity to Southeast Asia posed a point of vulnerability for Australian health officials. While smallpox was never endemic, (though epidemic), in Australia, it was endemic at various times and places across Southeast Asia. This shifting spectre of smallpox along the northern coastline was made even more acute for state and federal health officials because of the existing polyethnic relationships, communities, and economies. By vaccinating Indigenous peoples in this smallpox geography, they were envisioned and embedded into a ‘hygienic’ border for the protection of white Australia, entwining the two-prongs as one approach. In this article, we place public health into a recent scholarship that has ‘turned the map upside down’ to re-spatialise Australia’s history and geography to the north and its global connections, while demonstrating how particular coastlines and their connections were drawn into a national imaginary through a health lens.
Courting Civilians During Conflict: Evidence from Taliban Judges in Afghanistan
Authors: Grasse Donald , Sexton Renard , Wright Austin
Published: March 2024

Rebels regularly provide public services, especially legal services, but the consequences of such programs are unclear. We argue that rebel courts can boost civilian support for insurgency and augment attack capacity by increasing the legitimacy of the rebellion, creating a vested interest in rebel rule, or enabling rebel coercion of the civilian population. We study the impact of the Taliban's judiciary by leveraging cross-district and over-time variation in exposure to Taliban courts using a trajectory-balancing design. We find that rebel courts reduced civilian support for the government and increased it for the Taliban, and were associated with more attacks and more coalition casualties. Exploring mechanisms, we find that courts resolved major interpersonal disputes between civilians but also facilitated more insurgent intimidation of civilians, and that changes in public opinion are unlikely to have been driven solely by social desirability bias. Our findings help explain the logic of rebel courts and highlight the complex interactions between warfare and institutional development in weak states.
Simultaneously ‘national medicine’ and ‘East Asian medicine’: A cross-boundary network of medical exchange in wartime East Asia
Authors: Xia Yun
Published: March 2024

This article examines a transnational network of advocates for Kampō (traditional) medicine in Japan, occupied China, and Manchukuo during the Sino-Japanese War (1931–1945), shedding new light on collaborationism, Asianism, and the modernization of traditional medicine in East Asia. In the 1930s, despite deteriorating Sino-Japanese relations, the Kampō revival movement in Japan joined forces with the struggle to preserve traditional medicine in China. In 1938, the Association of East Asian Medicine was founded in Japan, gathering together Chinese, Korean, and Japanese advocates under the banner of ‘East Asian medicine’. This article delineates the evolution of what I call ‘medical Asianism’ and how it was institutionalized in different parts of the Japanese empire. Participants in this network differed in their priorities and ideological commitments, yet they tactfully utilized the Japanese imperial infrastructure and wartime circumstances to promote traditional medicine. Their work laid important intellectual and institutional foundations for the postwar development of traditional medicine across East Asia. This study also contributes to a more nuanced understandings of collaborationism. The type of collaboration examined in this article was preceded by a long history of intellectual exchange, based on a shared body of knowledge and morals, motivated by mutual empathy, and for a cause that was much valued in postwar Asia. As a result, unlike most Chinese collaborators who were prosecuted as ‘traitors’, protagonists in this study continued to prosper professionally and became valuable assets in the postwar rebuilding of Sino-Japanese relations.
Cosmopolitan collaboration and wartime collaborationism: The Chinese Maritime Customs Service and its staff, 1932–1941
Authors: Chang Chihyun , Kao Chiu-Ya
Published: March 2024

This article discusses the continuity between cosmopolitan collaboration and wartime collaborationism from 1932–1941 by exploring the Chinese Maritime Customs Service (CMCS) and its international staff. The CMCS managed China’s international trade and directed the custom houses in northern China before 1937, and in occupied China and free China from 1937–1941. The customs revenues generated by this international trade were pledged to service China’s international obligations. This article argues that both Chinese and Japanese staff members’ activities to maintain the status quo could be considered as wartime collaborationism from the perspectives of Japan, Manchukuo, and the Collaborationist and Chongqing governments, but all parties tolerated their activities until the outbreak of the Pacific War. The reason for this was that all parties benefitted from the CMCS’s management of international trade and its implementation of international obligations which had existed since the mid-nineteenth century. This article situates wartime collaborationism within the long-existing institutional network that was welcomed as cosmopolitan collaboration in the prewar, wartime, and postwar periods, rather than treating it as a unique wartime setup and ideology. Such a view also illuminates the postwar exchange of personnel and cooperation among former enemies, which grew out of prewar collaboration and wartime collaborationism.
Degrowth, global asymmetries, and ecosocial justice: Decolonial perspectives from Latin America
Authors: Lang Miriam
Published: March 2024

Degrowth literature predominantly states that degrowth strategies are meant from and for the Global North. While economic mainstream discourse suggests that the Global South still has to grow in terms of achieving development, degrowth proponents expect a reduction of material and energy throughput in the Global North to make ecological and conceptual space for the Global South to find its own paths toward ecosocial transformation. Based on a Latin American post-development and post-extractivist perspective and drawing on dependency theory, this article suggests another approach: first, it argues that the growth imperative, which in the peripheral world translates into the imperative to develop, also causes harm in societies of the Global South. Throughout Latin America, in the last decades, economic growth has mainly been achieved through extractivism with negative impacts, which are now being pushed further by green growth strategies. Second, I explore some possibilities for a cross-fertilisation between degrowth and International Relations scholarship, calling into question the assumption that degrowth in high-income countries would automatically ‘make space’ for the Global South to engage in self-determined paths of ecosocial transformation, as long as the structures, institutions, and rules of global governance and trade which secure profoundly asymmetric, colonial relations are not challenged.
“Breaking the Silence” Suicide Prevention Media Campaign in Oregon, April 7–14, 2019
Authors: Niederkrotenthaler Thomas , Metzler Hannah , Laido Zrinka , Till Benedikt , Lake Alison H. , Noble Emily , Chowdhury Saba , Gonzalez Frances , Garcia David , Draper John , Murphy Sean , Gould Madelyn
Published: March 2024

Abstract: Background: Between April 7 and 14, 2019, the “Breaking the Silence” media engagement campaign was launched in Oregon. Aims: We aimed to assess the consistency of media content related to the campaign with media guidelines and the quantitative footprint on Twitter (now X) over time. Method: Media items related to the campaign were analyzed regarding focus and consistency with media guidelines for suicide reporting and compared with other suicide-related reports published in the same time frame, as well as with reporting in Washington, the control region. Tweets related to the campaign were retrieved to assess the social media footprint. Results: There were n = 104 media items in the campaign month, mainly in the campaign week. Items typically used a narrative featuring suicide advocacy or policy/prevention programs. As compared to other items with a similar focus, they scored better on several protective characteristics listed in media recommendations. Stories of coping with adversity, however, were scarce. The social media footprint on Twitter was small. Limitations: Inability to make causal claims about campaign impact. Conclusion: Media items from the Breaking the Silence campaign appeared mainly consistent with media guidelines, but some aspects, such as stories of recovery, were under-represented.
Spatio‐Temporal Dynamics of Aboveground Biomass in China's Oasis Grasslands Between 1989 and 2021
Authors: Chen Peng , Wang Shuai , Liu Yanxu , Wang Yijia , Song Jiaxi , Tang Qiang , Yao Ying , Wang Yaping , Wu Xutong , Wei Fangli , Feng Siyuan
Published: March 2024

Grassland provides multiple ecosystem services and plays a key role in preventing desert encroachment and maintaining oasis stability. In China, the area of cropland in oases has expanded significantly in recent decades, which results in a rapid increase in agricultural water demand and encroachment on grassland subsistence space. However, our knowledge about how the expansion of cropland affects oasis grasslands remains limited. We used machine learning, temporal segmentation of spectral trajectories, and maximum covariance analysis to generate an annual oasis grassland aboveground biomass (AGB) data set at 30‐m resolution from 1989 to 2021 based on multiple remotely sensed and ground observation data sets, and investigated the dynamics of grassland AGB under cropland expansion in oases. We found that overall oasis grassland AGB increased significantly (0.3 gm−2 yr−1, P < 0.01) during 1989–2021, but trends in AGB were not consistent across basins. In the Yellow River, Turpan Hami, Qaidam, and southern Altai Mountains River Basins, AGB was dominated by a significant increase. Conversely, in all basins in southern Xinjiang, AGB showed a decreasing trend due to the rapid cropland expansion. Spatially, cropland expansion and AGB dynamics were strongly coupled. In regions characterized by agricultural concentration, grassland AGB benefitted from resource spillover via edge effects. However, in downstream areas or those with a low proportion of cropland, where most grasslands are distributed, the relationship between the two shifted to a trade‐off. Our study provides a scientific basis for identifying priority areas for ecological restoration and for science‐based planning of the scale of cropland in oases.
Interannual Variability of Subpolar Mode Water in the Subpolar North Atlantic
Authors: Stendardo I. , Buongiorno Nardelli B. , Durante S. , Iudicone D. , Kieke D.
Published: March 2024

Subpolar Mode Water (SPMW) is an important water mass originating in the eastern North Atlantic. Its formation, subject to modification through oceanic interior mixing, can directly influence the volume of water contributing to the Atlantic meridional overturning circulation. Utilizing observation‐based data sets spanning from 1993 to 2018, we estimated the formation rates and volume of SPMW within isopycnal layers and examined its temporal variability. Two complementary approaches were used to estimate the formation rate: a thermodynamic approach focusing on the air‐sea interactions and a kinematic approach involving volume transport from the mixed layer to the ocean's interior, including the entrainment/detrainment of the mixed layer itself. This is the first time that thermodynamic and kinematic approaches are applied to observation‐based data in the North Atlantic. Our results suggest a substantial role of diapycnal mixing in diluting the dense waters formed by air‐sea fluxes toward the range of SPMW densities. The study reveals a complex interplay of processes, with entrainment being the primary driver of subduction/obduction rates, while advection contributes to the overall small‐scale dynamics. Variations in the volume and location of SPMW formation are observed from year to year. Notably, when SPMW forms extensively in lighter isopycnal layers, the volume occupied by denser isopycnals decreases and vice versa. We attributed this compensation effect to a propagation signal, where formation in the lightest isopycnal bins influences the formation in denser isopycnal bins with a delay of a few years, emphasizing the circulation's role in shaping the SPMW distribution.
Reconciling Modeled and Observed Δ17O(NO3−) in Beijing Winter Haze With Heterogeneous Chlorine Chemistry
Authors: Zhang Zhongyi , Jiang Zhuang , Zhou Tao , Geng Lei
Published: March 2024

The fine particulate matter (PM2.5) in Chinese megacities has declined significantly after the implementation of strict mitigation strategies since 2013. However, the concentration of wintertime nitrate in PM2.5 (p‐NO3‐) has only changed slightly and became the dominant inorganic component despite considerable precursor emission reductions. Discerning chemical mechanisms leading to nitrate growth during haze events is critical to implementing effective pollution mitigation policies. The oxygen isotope anomaly of nitrate (Δ17O(NO3−)) is a powerful means to distinguish nitrate formation mechanisms. Nevertheless, the observed high Δ17O(NO3−) values were significantly underestimated by chemical transport models during extreme haze events (PM2.5 > 225 μg m−3) in Beijing, indicating an incomplete understanding of nitrate chemistry. To reconcile this model‐observation discrepancy, we compiled reported Δ17O(NO3−) data in Beijing haze along with relevant observational parameters (e.g., hydroxyl (OH) reactivity, peroxyl radical concentrations), then tested assumptions on Δ17O of key precursors (e.g., OH and nitrogen dioxide (NO2)), recalculated Δ17O(NO3−) and compared them with observations. Our results indicate that considering heterogeneous dinitrogen pentoxide reactions on chlorine‐containing aerosols (N2O5 + Cl− chemistry) with a nitryl chloride (ClNO2) yield of ∼0.75 can explain the observed high Δ17O(NO3−) during extreme haze events. According to the Δ17O(NO3−) data, on average this heterogeneous N2O5 + Cl− chemistry can explain ∼60% of nighttime nitrate production and make daytime and nocturnal pathways equally important in winter Beijing haze (PM2.5 > 75 μg m−3). Our results highlight the critical role of reactive chlorine chemistry in air pollution/chemistry in inland cities.
Impacts of Northward Typhoons on Autumn Haze Pollution Over North China Plain
Authors: Lin Haoxian , Ding Ke , Huang Xin , Lou Sijia , Xue Lian , Wang Zilin , Ma Yue , Ding Aijun
Published: March 2024

Although air quality in China has improved substantially over recent years, haze pollution events still occur frequently, especially over the North China Plain (NCP). Previous studies showed that typhoons are conducive to regional pollution events in eastern China; however, the underlying mechanism and quantitative understanding of the typhoons' impact on haze pollution remain unclear there. Here, based on ground‐based and satellite observations, reanalysis data, and model simulations, we show that northward typhoons approaching China are essential for autumn haze pollution over NCP. Elevated relative humidity levels and enhanced pollution accumulation, caused by northward typhoons and the corresponding high‐pressure systems, are responsible for the pollution enhancements over NCP. Compared with episodes without typhoon influence, cities near Taihang and Yan Mountain suffer from heavier haze pollution when typhoons approach, with PM2.5 concentrations increasing from 87.1 to 106.4 μg m−3. More water vapor from the Yellow and Bohai Seas and pollutants from eastern China are transported to these cities by typhoon‐induced southeasterly wind anomalies, facilitating the chemical formation of aerosols there. In addition, by the block of mountains, these southeasterly wind anomalies also lead to stronger local accumulation over cities and an elevation of pollutants along the mountains. What is more, with the implementation of emission reduction, the relative changes of PM2.5 concentrations between typhoon‐induced episodes and no‐typhoon episodes increase. This work highlights the importance of understanding the impact of synoptical weather on PM2.5 transport, accumulation, and formation processes in haze pollution mitigation in eastern China.
Global Forest Plantations Mapping and Biomass Carbon Estimation
Authors: Xu Hongtao , He Bin , Guo Lanlan , Yan Xing , Zeng Yelu , Yuan Wenping , Zhong Ziqian , Tang Rui , Yang Yang , Liu Huiming , Chen Yaning
Published: March 2024

Management of forest plantations is a natural based solution to the global‐scale mitigation of climate change; however, the role of carbon sequestration remains poorly understood, and this is hampered by a lack of detailed distribution on the global forest plantations. For the first time, we generated a global spatial distribution for forest plantations (GSDFP) during 2015 at a spatial resolution of 250 m using hierarchical extraction based on a machine learning algorithm taking time series of MODIS and ALOS PALSAR imagery as the source data, and finally estimates the biomass carbon stored in forest plantations. The resultant map was validated using reference samples visually interpreted, as well as reference samples collected from previous studies, and inter‐compared with statistical forest plantation area and regional forest plantation maps, and the GSDFP was found to accurately represent the spatial distribution of forest plantations around the globe. Globally, forest plantations account for 7.35% (360.22 × 104 km2) of the global forest area and are estimated to contribute 4.60% (12.85 Pg C) of global forest biomass carbon. The forest plantation map and biomass carbon stock estimates will assist forest management and provide a benchmark for the estimation of the forest plantation carbon sink.
Diffuse Fertilization Effect in Maize and Soybean Is Driven by Improved Light Use Efficiency Rather Than by Light Absorption
Authors: Zhang Zhaoyang , Zhu Kailing , Fan Meng , Wang Quan , Tan Yunhui
Published: March 2024

Diffuse radiation can improve the efficiency of terrestrial carbon uptake. Both light absorption and canopy light use efficiency (LUE) are affected by the fraction of diffuse radiation (DF). However, the relative contribution of these two factors to the diffuse fertilization effect (DFE) in the short term is not well understood. To investigate the mechanism of DFE in cropland, we collected gross primary productivity, fraction of absorbed photosynthetically active radiation (FAPAR), and other meteorological parameters from June to August in three crop sites. Our results indicated that the short‐term DFE in soybean and maize was primarily driven by the improvement of LUE rather than light absorption. The LUE increased significantly with DF, while the FAPAR changed a little with DF. The increased DF is typically associated with reduced photosynthetically active radiation (PAR). Our results showed that the potential gain in light absorption and canopy LUE from DF could not fully offset the reduced PAR in soybean and maize. The findings in this study contribute to our understanding of the mechanisms underlying the DFE.
Evaluating Streamflow Forecasts in Hydro‐Dominated Power Systems—When and Why They Matter
Authors: Koh Rachel , Galelli Stefano
Published: March 2024

The value of seasonal streamflow forecasts for the hydropower industry has long been assessed by considering metrics related to hydropower availability. However, this approach overlooks the role played by hydropower dams within the power grid, therefore providing a myopic view of how forecasts could improve the operations of large‐scale power systems. With the aim of understanding how the value of streamflow forecasts penetrates through the power grid, we developed a coupled‐water energy model that is subject to reservoir inflow forecasts with different levels of predictive performance. We implement the modeling framework on a real‐world case study based on the Cambodian grid, which relies on hydropower, coal, oil, and imports from neighboring countries. In particular, we evaluate the performance in terms of metrics selected from both the reservoir and power systems, including available and dispatched hydropower, power production costs, CO2 emissions, and transmission line congestion. Through this framework, we demonstrate that streamflow forecasts can positively impact the operations of hydro‐dominated power systems, especially during the transition from wet to dry seasons. Moreover, we show that the value largely varies with the specific metric of performance at hand as well as the level of operational integration between water and power systems.
The effect of family integrated care on preparing parents with premature infants hospitalized in the neonatal intensive care unit for discharge
Authors: Tiryaki Öznur , Çınar Nursan , Caner İbrahim
Published: March 2024

Objective

The study was designed as a randomized controlled experimental trial to determine the effect of the Family Integrated Care (FICare) model on the readiness of parents whose infants were hospitalized in the neonatal intensive care unit (NICU) for discharge and home care of the infants.

Study design

Parents in the intervention group received FICare, and parents in the control group received standard care.

Results

The total mean score of the mothers and fathers in the intervention group regarding readiness for discharge and home care was higher than that of the control group, and a significant difference was observed. A statistically significant difference was found in terms of discharge weight, the day of first enteral feeding, and first breast milk.

Conclusion

The FICare model was observed to enhance the readiness of mothers and fathers for discharge and home care and positively affect the infant’s weight gain, the status of breastfeeding and the continuation of nutrition.

Clinical trial registration

Registered on ClinicalTrials.gov (Identifiers: NCT04478162 Unique Protocol ID: 16214662/050.01.04/14) on 17/07/2020.
Insular cortex subregions have distinct roles in cued heroin seeking after extinction learning and prolonged withdrawal in rats
Authors: McGregor Matthew S. , Cosme Caitlin V. , LaLumiere Ryan T.
Published: March 2024

Evidence indicates that the anterior (aIC), but not posterior (pIC), insular cortex promotes cued reinstatement of cocaine seeking after extinction in rats. It is unknown whether these subregions also regulate heroin seeking and whether such involvement depends on prior extinction learning. To address these questions, we used baclofen and muscimol (BM) to inactivate the aIC or pIC bilaterally during a seeking test after extinction or prolonged withdrawal from heroin. Male Sprague-Dawley rats in the extinction groups underwent 10+ days of heroin self-administration, followed by 6+ days of extinction sessions, and subsequent cued or heroin-primed reinstatement. Results indicate that aIC inactivation increased cued reinstatement of heroin seeking after extinction, whereas pIC inactivation prevented cued reinstatement. To determine whether these effects were extinction-dependent, we conducted a subsequent study using both sexes with prolonged withdrawal. Male and female rats in the withdrawal groups underwent 10+ days of heroin self-administration, followed by cued seeking tests after 1 and 14 days of homecage withdrawal to measure incubation of heroin craving. In this case, the findings indicate that aIC inactivation had no effect on incubation of heroin craving after withdrawal in either sex, whereas pIC inactivation decreased heroin craving only in males. These findings suggest that the aIC and pIC have opposing roles in suppressing vs promoting cued heroin seeking after extinction and that these roles are distinct from those in cocaine seeking. Moreover, the incubation of craving results suggest that new contingency learning is necessary to recruit the aIC in cued heroin seeking.
Hippo pathway in non-small cell lung cancer: mechanisms, potential targets, and biomarkers
Authors: Liang Hongge , Xu Yan , Zhao Jing , Chen Minjiang , Wang Mengzhao
Published: March 2024

Lung cancer is the primary contributor to cancer-related deaths globally, and non-small cell lung cancer (NSCLC) constitutes around 85% of all lung cancer cases. Recently, the emergence of targeted therapy and immunotherapy revolutionized the treatment of NSCLC and greatly improved patients’ survival. However, drug resistance is inevitable, and extensive research has demonstrated that the Hippo pathway plays a crucial role in the development of drug resistance in NSCLC. The Hippo pathway is a highly conserved signaling pathway that is essential for various biological processes, including organ development, maintenance of epithelial balance, tissue regeneration, wound healing, and immune regulation. This pathway exerts its effects through two key transcription factors, namely Yes-associated protein (YAP) and transcriptional co-activator PDZ-binding motif (TAZ). They regulate gene expression by interacting with the transcriptional-enhanced associate domain (TEAD) family. In recent years, this pathway has been extensively studied in NSCLC. The review summarizes a comprehensive overview of the involvement of this pathway in NSCLC, and discusses the mechanisms of drug resistance, potential targets, and biomarkers associated with this pathway in NSCLC.
The potential key role of choroidal non-perfusion and rod degeneration in the pathogenesis of macular neovascularization type 3
Authors: Haj Najeeb Bilal , Schmidt-Erfurth Ursula
Published: March 2024

Macular neovascularization type 3 (MNV3) is a multifactorial disease with distinct epidemiological, clinical, pathomorphological and topographical characteristics. This review of the literature discusses the latest experimental and clinical outcomes that could explain the pathogenesis of retinal neovascularization. Although patients with MNV3 are usually older than those with MNV1 or 2, their lesions do not coexist with, precede, or follow other types in the same eye. The regional distribution of MNV3 lesions is characterized as confined to the parafoveal macula without any involvement of the rod-free foveal area. Focal outer retinal atrophy and choroidal non-perfusion are the main structural features that occur prior to the development of retinal neovascularization. Also, histological and experimental studies of MNV3 and other non-neovascular age-related macular degeneration diseases complicated with MNV3-like lesions strongly suggest rod degeneration contributes to the pathogenesis. Therefore, the retinal neovascularization in MNV3 has a different pathogenesis from the choroidal neovascularization in MNV1 and 2 and emerging evidence indicates that choroidal non-prefusion and rod degeneration play a key role in the pathogenesis of MNV3. Accordingly, we suggest a sequence of pathological events that start with choroidal non-perfusion due to advanced age followed by hypoxia of the outer retina at the parafoveal area. This induces a remarkable degeneration of rods that triggers the growth of retinal neovascularization due to the imbalance of the angiogenic factors in the outer retina.
Synthesis of meta-carbonyl phenols and anilines
Authors: Zhao Bao-Yin , Jia Qiong , Wang Yong-Qiang
Published: March 2024

Phenols and anilines are of extreme importance for medicinal chemistry and material science. The development of efficient approaches to prepare both compounds has thus long been a vital research topic. The utility of phenols and anilines directly reflects the identity and pattern of substituents on the benzenoid ring. Electrophilic substitutions remain among the most powerful synthetic methods to substituted phenols and anilines, yet in principle achieving ortho- and para-substituted products. Therefore, the selective preparation of meta-substituted phenols and anilines is the most significant challenge. We herein report an efficient copper-catalyzed dehydrogenation strategy to exclusively synthesize meta-carbonyl phenols and anilines from carbonyl substituted cyclohexanes. Mechanistic studies indicate that this transformation undergoes a copper-catalyzed dehydrogenation/allylic hydroxylation or amination/oxidative dehydrogenation/aromatization cascade process.
Ferroelectric compute-in-memory annealer for combinatorial optimization problems
Authors: Yin Xunzhao , Qian Yu , Vardar Alptekin , Günther Marcel , Müller Franz , Laleni Nellie , Zhao Zijian , Jiang Zhouhang , Shi Zhiguo , Shi Yiyu , Gong Xiao , Zhuo Cheng , Kämpfe Thomas , Ni Kai
Published: March 2024

Computationally hard combinatorial optimization problems (COPs) are ubiquitous in many applications. Various digital annealers, dynamical Ising machines, and quantum/photonic systems have been developed for solving COPs, but they still suffer from the memory access issue, scalability, restricted applicability to certain types of COPs, and VLSI-incompatibility, respectively. Here we report a ferroelectric field effect transistor (FeFET) based compute-in-memory (CiM) annealer for solving larger-scale COPs efficiently. Our CiM annealer converts COPs into quadratic unconstrained binary optimization (QUBO) formulations, and uniquely accelerates in-situ the core vector-matrix-vector (VMV) multiplication operations of QUBO formulations in a single step. Specifically, the three-terminal FeFET structure allows for lossless compression of the stored QUBO matrix, achieving a remarkably 75% chip size saving when solving Max-Cut problems. A multi-epoch simulated annealing (MESA) algorithm is proposed for efficient annealing, achieving up to 27% better solution and ~ 2X speedup than conventional simulated annealing. Experimental validation is performed using the first integrated FeFET chip on 28nm HKMG CMOS technology, indicating great promise of FeFET CiM array in solving general COPs.
A fast-acting lipid checkpoint in G1 prevents mitotic defects
Authors: Köberlin Marielle S. , Fan Yilin , Liu Chad , Chung Mingyu , Pinto Antonio F. M. , Jackson Peter K. , Saghatelian Alan , Meyer Tobias
Published: March 2024

Lipid synthesis increases during the cell cycle to ensure sufficient membrane mass, but how insufficient synthesis restricts cell-cycle entry is not understood. Here, we identify a lipid checkpoint in G1 phase of the mammalian cell cycle by using live single-cell imaging, lipidome, and transcriptome analysis of a non-transformed cell. We show that synthesis of fatty acids in G1 not only increases lipid mass but extensively shifts the lipid composition to unsaturated phospholipids and neutral lipids. Strikingly, acute lowering of lipid synthesis rapidly activates the PERK/ATF4 endoplasmic reticulum (ER) stress pathway that blocks cell-cycle entry by increasing p21 levels, decreasing Cyclin D levels, and suppressing Retinoblastoma protein phosphorylation. Together, our study identifies a rapid anticipatory ER lipid checkpoint in G1 that prevents cells from starting the cell cycle as long as lipid synthesis is low, thereby preventing mitotic defects, which are triggered by low lipid synthesis much later in mitosis.
Pulse potential mediated selectivity for the electrocatalytic oxidation of glycerol to glyceric acid
Authors: Chen Wei , Zhang Liang , Xu Leitao , He Yuanqing , Pang Huan , Wang Shuangyin , Zou Yuqin
Published: March 2024

Preventing the deactivation of noble metal-based catalysts due to self-oxidation and poisonous adsorption is a significant challenge in organic electro-oxidation. In this study, we employ a pulsed potential electrolysis strategy for the selective electrocatalytic oxidation of glycerol to glyceric acid over a Pt-based catalyst. In situ Fourier-transform infrared spectroscopy, quasi-in situ X-ray photoelectron spectroscopy, and finite element simulations reveal that the pulsed potential could tailor the catalyst’s oxidation and surface micro-environment. This prevents the overaccumulation of poisoning intermediate species and frees up active sites for the re-adsorption of OH adsorbate and glycerol. The pulsed potential electrolysis strategy results in a higher glyceric acid selectivity (81.8%) than constant-potential electrocatalysis with 0.7 VRHE (37.8%). This work offers an efficient strategy to mitigate the deactivation of noble metal-based electrocatalysts.
Autophagy formation, microtubule disorientation, and alteration of ATG8 and tubulin gene expression under simulated microgravity in Arabidopsis thaliana
Authors: Yemets Alla , Shadrina Ruslana , Blume Rostyslav , Plokhovska Svitlana , Blume Yaroslav
Published: March 2024

Autophagy plays an important role in plant growth and development, pathogen invasion and modulates plant response and adaptation to various abiotic stress stimuli. The biogenesis and trafficking of autophagosomes involve microtubules (MTs) as important actors in the autophagic process. However, initiation of autophagy in plants under microgravity has not been previously studied. Here we demonstrate how simulated microgravity induces autophagy development involving microtubular reorganization during period of autophagosome formation. It was shown that induction of autophagy with maximal autophagosome formation in root cells of Arabidopsis thaliana is observed after 6 days of clinostating, along with MT disorganization, which leads to visible changes in root morphology. Gradual decrease of autophagosome number was indicated on 9th and 12th days of the experiment as well as no significant re-orientation of MTs were identified. Respectively, analysis of α- and β-tubulins and ATG8 gene expression was carried out. In particular, the most pronounced increase of expression on both 6th and 9th days in response to simulated microgravity was detected for non-paralogous AtATG8b, AtATG8f, AtATG8i, and AtTUA2, AtTUA3 genes, as well as for the pair of β-tubulin duplicates, namely AtTUB2 and AtTUB3. Overall, the main autophagic response was observed after 6 and 9 days of exposure to simulated microgravity, followed by adaptive response after 12 days. These findings provide a key basis for further studies of cellular mechanisms of autophagy and involvement of cytoskeletal structures in autophagy biogenesis under microgravity, which would enable development of new approaches, aimed on enhancing plant adaptation to microgravity.
Turning alterations detected by mobile health technology in idiopathic REM sleep behavior disorder
Authors: Zatti Cinzia , Pilotto Andrea , Hansen Clint , Rizzardi Andrea , Catania Marcello , Romijnders Robbin , Purin Leandro , Pasolini Maria P. , Schaeffer Eva , Galbiati Andrea , Ferini-Strambi Luigi , Berg Daniela , Maetzler Walter , Padovani Alessandro
Published: March 2024

Idiopathic REM sleep Behavior Disorder (iRBD) is a condition at high risk of developing Parkinson’s disease (PD) and other alpha-synucleinopathies. The aim of the study was to evaluate subtle turning alterations by using Mobile health technology in iRBD individuals without subthreshold parkinsonism. A total of 148 participants (23 persons with polysomnography-confirmed iRBD without subthreshold parkinsonism, 60 drug-naïve PD patients, and 65 age-matched controls were included in this prospective cross-sectional study. All underwent a multidimensional assessment including cognitive and non-motor symptoms assessment. Then a Timed-Up-and-Go test (TUG) at normal and fast speed was performed using mobile health technology on the lower back (Rehagait®, Hasomed, Germany). Duration, mean, and peak angular velocities of the turns were compared using a multivariate model correcting for age and sex. Compared to controls, PD patients showed longer turn durations and lower mean and peak angular velocities of the turns in both TUGs (all p ≤ 0.001). iRBD participants also showed a longer turn duration and lower mean (p = 0.006) and peak angular velocities (p < 0.001) compared to controls, but only in the TUG at normal speed. Mobile health technology assessment identified subtle alterations of turning in subjects with iRBD in usual, but not fast speed. Longitudinal studies are warranted to evaluate the value of objective turning parameters in defining the risk of conversion to PD in iRBD and in tracking motor progression in prodromal PD.
Making drugs from T cells: The quantitative pharmacology of engineered T cell therapeutics
Authors: Kirouac Daniel C. , Zmurchok Cole , Morris Denise
Published: March 2024

Engineered T cells have emerged as highly effective treatments for hematological cancers. Hundreds of clinical programs are underway in efforts to expand the efficacy, safety, and applications of this immuno-therapeutic modality. A primary challenge in developing these “living drugs” is the complexity of their pharmacology, as the drug product proliferates, differentiates, traffics between tissues, and evolves through interactions with patient immune systems. Using publicly available clinical data from Chimeric Antigen Receptor (CAR) T cells, we demonstrate how mathematical models can be used to quantify the relationships between product characteristics, patient physiology, pharmacokinetics and clinical outcomes. As scientists work to develop next-generation cell therapy products, mathematical models will be integral for contextualizing data and facilitating the translation of product designs to clinical strategy.
Biomimetic versus arbitrary motor control strategies for bionic hand skill learning
Authors: Schone Hunter R. , Udeozor Malcolm , Moninghoff Mae , Rispoli Beth , Vandersea James , Lock Blair , Hargrove Levi , Makin Tamar R. , Baker Chris I.
Published: March 2024

A long-standing engineering ambition has been to design anthropomorphic bionic limbs: devices that look like and are controlled in the same way as the biological body (biomimetic). The untested assumption is that biomimetic motor control enhances device embodiment, learning, generalization and automaticity. To test this, we compared biomimetic and non-biomimetic control strategies for non-disabled participants when learning to control a wearable myoelectric bionic hand operated by an eight-channel electromyography pattern-recognition system. We compared motor learning across days and behavioural tasks for two training groups: biomimetic (mimicking the desired bionic hand gesture with biological hand) and arbitrary control (mapping an unrelated biological hand gesture with the desired bionic gesture). For both trained groups, training improved bionic limb control, reduced cognitive reliance and increased embodiment over the bionic hand. Biomimetic users had more intuitive and faster control early in training. Arbitrary users matched biomimetic performance later in training. Furthermore, arbitrary users showed increased generalization to a new control strategy. Collectively, our findings suggest that biomimetic and arbitrary control strategies provide different benefits. The optimal strategy is probably not strictly biomimetic, but rather a flexible strategy within the biomimetic-to-arbitrary spectrum, depending on the user, available training opportunities and user requirements.
In vitro and in vivo investigation of a thyroid hormone system-specific interaction with triazoles
Authors: Kadic Asya , Oles Patricia , Fischer Benjamin Christian , Reetz Anne Elisabeth , Sylla Boubacar Sidiki , Feiertag Katreece , Ritz Vera , Heise Tanja , Marx-Stoelting Philip , Tralau Tewes , Renko Kostja , Solano Marize de Lourdes Marzo
Published: March 2024

Alterations in thyroid hormones (TH) and thyroid-stimulating hormone levels are frequently found following exposure to chemicals of concern. Dysregulation of TH levels can severely perturb physiological growth, metabolism, differentiation, homeostasis in the adult and developmental processes in utero. A frequently identified mode of action for this interaction is the induction of hepatic detoxification mechanisms (e.g. SULTs and UGTs), which lead to TH conjugation and elimination and therefore interfere with hormonal homeostasis, fulfilling the endocrine disruptors (EDs) definition. A short-term study in rats with dietary exposure to cyproconazole, epoxiconazole and prochloraz was conducted and hepatocyte hypertrophy, hepatic UGT activity and Phase 1/2 gene expression inductions were observed together with changes in TH levels and thyroid follicular hypertrophy and hyperplasia. To test for specific interaction with the thyroid hormone system, in vitro assays were conducted covering thyroidal I-uptake (NIS), TH transmembranal transport via MCT8 and thyroid peroxidase (TPO) function. Assays for iodothyronine deiodinases (DIO1–DIO3) and iodotyrosine deiodinase (DEHAL1) were included, and from the animal experiment, Dio1 and Dehal1 activities were measured in kidney and liver as relevant local indicators and endpoints. The fungicides did not affect any TH-specific KEs, in vitro and in vivo, thereby suggesting hepatic conjugation as the dominant MoA.