bluuebunny/crossref_metadata_2025 huggingface dataset:
Novel Mesoporous Organosilicas with Task Ionic Liquids: Properties and High Adsorption Performance for Pb(II)
Authors: Wieszczycka Karolina , Filipowiak Kinga , Dudzinska Patrycja , Nowicki Marek , Siwińska-Ciesielczyk Katarzyna , Jesionowski Teofil
Published: February 2022

Removal of toxic contaminants such as Pb(II) from waste solutions is environmentally requested. Therefore, in this paper, for potential novel sorbents, mesoporous ionic liquid-functionalized silicas were synthesized and tested for the removal of Pb(II) from aqueous solutions. The successful synthesis of the adsorbents was proved by nuclear magnetic resonance (29Si and 13C NMR), Fourier transform infrared spectroscopy (FTIR), and elemental analysis. The structural and textural properties were determined using scanning electron microscopy (SEM), X-ray diffraction (XRD), high-resolution transmission electron microscopy (TEM), and low-temperature N2 sorption, and the result showed that the applied procedure made it possible to obtain highly ordered particles with a two-dimensional mesostructure. The effects of several parameters including initial pH, contact time, adsorption temperature, and Pb(II) concentration were studied in detail and were discussed to evaluate the adsorption properties of the fabricated materials towards Pb(II). The obtained results confirmed a very high potential of the sorbents; however, the adsorption properties depend on the structure and amounts of the functional group onto fabricated materials. The sample ILS-Ox3-40 showed fast kinetics (equilibrium reached within 10 min) and capacity of 172 mg/g, and that makes it a promising sorbent for the cleanup of water contaminated by lead. It was also indicated that, regardless on structure of the tested materials, the Pb(II) removal was spontaneous and exothermic. The fabricated mesoporous silicas exhibited that they were easy to regenerate and had excellent reusability.
Phytochemical and In Silico ADME/Tox Analysis of Eruca sativa Extract with Antioxidant, Antibacterial and Anticancer Potential against Caco-2 and HCT-116 Colorectal Carcinoma Cell Lines
Authors: Awadelkareem Amir Mahgoub , Al-Shammari Eyad , Elkhalifa Abd Elmoneim O. , Adnan Mohd , Siddiqui Arif Jamal , Snoussi Mejdi , Khan Mohammad Idreesh , Azad Z R Azaz Ahmad , Patel Mitesh , Ashraf Syed Amir
Published: February 2022

Eruca sativa Mill. (E. sativa) leaves recently grabbed the attention of scientific communities around the world due to its potent bioactivity. Therefore, the present study investigates the metabolite profiling of the ethanolic crude extract of E. sativa leaves using high resolution-liquid chromatography-mass spectrometry (HR-LC/MS), including antibacterial, antioxidant and anticancer potential against human colorectal carcinoma cell lines. In addition, computer-aided analysis was performed for determining the pharmacokinetic properties and toxicity prediction of the identified compounds. Our results show that E. sativa contains several bioactive compounds, such as vitamins, fatty acids, alkaloids, flavonoids, terpenoids and phenols. Furthermore, the antibacterial assay of E. sativa extract showed inhibitory effects of the tested pathogenic bacterial strains. Moreover, the antioxidant activity of 2,2-diphenyl-1-picrylhydrazyl (DPPH) and hydrogen peroxide (H2O2) were found to be IC50 = 66.16 μg/mL and 76.05 μg/mL, respectively. E. sativa also showed promising anticancer activity against both the colorectal cancer cells HCT-116 (IC50 = 64.91 μg/mL) and Caco-2 (IC50 = 83.98 μg/mL) in a dose/time dependent manner. The phytoconstituents identified showed promising pharmacokinetics properties, representing a valuable source for drug or nutraceutical development. These investigations will lead to the further exploration as well as development of E. sativa-based nutraceutical products.
Drug Repurposing and De Novo Drug Discovery of Protein Kinase Inhibitors as New Drugs against Schistosomiasis
Authors: Pereira Moreira Bernardo , Weber Michael H. W. , Haeberlein Simone , Mokosch Annika S. , Spengler Bernhard , Grevelding Christoph G. , Falcone Franco H.
Published: February 2022

Schistosomiasis is a neglected tropical disease affecting more than 200 million people worldwide. Chemotherapy relies on one single drug, praziquantel, which is safe but ineffective at killing larval stages of this parasite. Furthermore, concerns have been expressed about the rise in resistance against this drug. In the absence of an antischistosomal vaccine, it is, therefore, necessary to develop new drugs against the different species of schistosomes. Protein kinases are important molecules involved in key cellular processes such as signaling, growth, and differentiation. The kinome of schistosomes has been studied and the suitability of schistosomal protein kinases as targets demonstrated by RNA interference studies. Although protein kinase inhibitors are mostly used in cancer therapy, e.g., for the treatment of chronic myeloid leukemia or melanoma, they are now being increasingly explored for the treatment of non-oncological conditions, including schistosomiasis. Here, we discuss the various approaches including screening of natural and synthetic compounds, de novo drug development, and drug repurposing in the context of the search for protein kinase inhibitors against schistosomiasis. We discuss the status quo of the development of kinase inhibitors against schistosomal serine/threonine kinases such as polo-like kinases (PLKs) and mitogen-activated protein kinases (MAP kinases), as well as protein tyrosine kinases (PTKs).
PLLA Coating of Active Implants for Dual Drug Release
Authors: Wulf Katharina , Goblet Madeleine , Raggl Stefan , Teske Michael , Eickner Thomas , Lenarz Thomas , Grabow Niels , Paasche Gerrit
Published: February 2022

Cochlear implants, like other active implants, rely on precise and effective electrical stimulation of the target tissue but become encapsulated by different amounts of fibrous tissue. The current study aimed at the development of a dual drug release from a PLLA coating and from the bulk material to address short-term and long-lasting release of anti-inflammatory drugs. Inner-ear cytocompatibility of drugs was studied in vitro. A PLLA coating (containing diclofenac) of medical-grade silicone (containing 5% dexamethasone) was developed and release profiles were determined. The influence of different coating thicknesses (2.5, 5 and 10 µm) and loadings (10% and 20% diclofenac) on impedances of electrical contacts were measured with and without pulsatile electrical stimulation. Diclofenac can be applied to the inner ear at concentrations of or below 4 × 10−5 mol/L. Release of dexamethasone from the silicone is diminished by surface coating but not blocked. Addition of 20% diclofenac enhances the dexamethasone release again. All PLLA coatings serve as insulator. This can be overcome by using removable masking on the contacts during the coating process. Dual drug release with different kinetics can be realized by adding drug-loaded coatings to drug-loaded silicone arrays without compromising electrical stimulation.
Antibacterial Effect of Lemongrass (Cymbopogon citratus) against the Aetiological Agents of Pitted Keratolyis
Authors: Schweitzer Bettina , Balázs Viktória Lilla , Molnár Szilárd , Szögi-Tatár Bernadett , Böszörményi Andrea , Palkovics Tamás , Horváth Györgyi , Schneider György
Published: February 2022

Pitted keratolysis (PK) is a bacterial skin infection mostly affecting the pressure-bearing areas of the soles, causing unpleasant symptoms. Antibiotics are used for therapy, but the emergence of antiobiotic resistance, makes the application of novel topical therapeutic agents necessary. The antibacterial effects of 12 EOs were compared in the first part of this study against the three known aetiological agents of PK (Kytococcus sedentarius, Dermatophilus congolensis and Bacillus thuringiensis). The results of the minimal inhibitory concentration, minimal bactericidal concentration and spore-formation inhibition tests revealed that lemongrass was the most effective EO against all three bacterium species and was therefore chosen for further analysis. Seventeen compounds were identified with solid-phase microextraction followed by gas chromatography–mass spectrometry (HS-SPME/GC-MS) analysis while thin-layer chromatography combined with direct bioautography (TLC-BD) was used to detect the presence of antibacterially active compounds. Citral showed a characteristic spot at the Rf value of 0.47, while the HS-SPME/GC-MS analysis of an unknown spot with strong antibacterial activity revealed the presence of α-terpineol, γ-cadinene and calamenene. Of these, α-terpineol was confirmed to possess an antimicrobial effect on all three bacterium species associated with PK. Our study supports the hypothesis that, based on their spectrum, EO-based formulations have potent antibacterial effects against PK and warrant further investigation as topical therapeutics.
Deep Eutectic Solvents as Phase Change Materials in Solar Thermal Power Plants: Energy and Exergy Analyses
Authors: Peyrovedin Hamed , Haghbakhsh Reza , Duarte Ana Rita C. , Shariati Alireza
Published: February 2022

Nowadays, producing energy from solar thermal power plants based on organic Rankine cycles coupled with phase change material has attracted the attention of researchers. Obviously, in such solar plants, the physical properties of the utilized phase change material (PCM) play important roles in the amounts of generated power and the efficiencies of the plant. Therefore, to choose the best PCM, various factors must be taken into account. In addition, considering the physical properties of the candidate PCM, the issue of environmental sustainability should also be considered when making the selection. Deep eutectic solvents (DESs) are novel green solvents, which, in addition to having various favorable characteristics, are environmentally sustainable. Accordingly, in this work, the feasibility of using seven different deep eutectic solvents as the PCMs of solar thermal power plants with organic Rankine cycles was investigated. By applying exergy and energy analyses, the performances of each were compared to paraffin, which is a conventional PCM. According to the achieved results, most of the investigated “DES cycles” produce more power than the conventional cycle using paraffin as its PCM. Furthermore, lower amounts of the PCM are required when paraffin is replaced by a DES at the same operational conditions.
Saprophytic Bacillus Accelerates the Release of Effective Components in Agarwood by Degrading Cellulose
Authors: Yang Huizhen , He Runying , Cui Yao , Li Ying , Ge Xizhen
Published: February 2022

The value of Agarwood increases with time due to the gradual release of its major components, but the mechanism behind this remains unclear. Herein we reveal that the potential driving force of this process is the degradation of cellulose in Agarwood by its saprophytic Bacillus subtilis. We selected 10-year-old Agarwood from different places and then isolated the saprophytic bacteria. We confirmed these bacteria from different sources are all Bacillus and confirmed they can degrade cellulose, and the highest cellulase activity reached 0.22 U/mL. By co-cultivation of the bacterium and Agarwood powder, we found that three of the strains could release the effective components of Agarwood, while they had little effect in increasing the same components in living Aquilaria sinensis. Finally, we demonstrated that these saprophytic Bacillus subtilis have similar effects on Zanthoxylum bungeanum Maxim and Dalbergiaod orifera T. Chen, but not on Illicium verum Hook. f, Cinnamomum cassia Presl and Phellodendron chinense Schneid. In conclusion, our experiment revealed that the saprophytic Bacillus release the effective components of Agarwood by degrading cellulose, and we provide a promising way to accelerate this process by using this bacterial agent.
In Situ Growth of PbS/PbI2 Heterojunction and Its Photoelectric Properties
Authors: Yang Shangxun , Han Jun , Zhang Jin , Kong Yingxiu , Liu Huan
Published: February 2022

In this paper, PbI2 thin films with a uniform surface morphology and compact structure were prepared by adjusting the spin coating process parameters. On such a basis, the PbS/PbI2 heterojunction was fabricated on the PbI2 surface by the method of in situ chemical replacement growth. The results show that the PbS/PbI2 heterojunction grown by this method has a clear interface and is closely combined. The introduction of a PbS layer enables its spectral response range to cover the visible and near-infrared regions. Compared with the PbI2 thin film device, its responsivity is increased by three orders of magnitude, its response time reduced by 42%, and its recovery time decreased by nearly 1/2 under 450 nm illumination. In the case that there is no response for the PbI2 thin film device under 980 nm illumination, the specific detectivity of the PbS/PbI2 heterojunction device still amounts to 1.8 × 108 Jones. This indicates that the in situ chemical replacement is a technique that can construct a high-quality heterojunction in a simple process. PbS/PbI2 heterojunction fabricated by this method has a visible–near-infrared light detection response range, which provides a new idea for creating visible–near-infrared common-path detection systems.
Liquid-Phase Exfoliation of Ta2NiS5 and Its Application in Near-Infrared Mode-Locked Fiber Lasers with Evanescent Field Interactions and Passively Q-Switched Bulk Laser
Authors: Liu Shunxiang , Huang Hongfu , Lu Jinsheng , Xu Ning , Qu Junle , Wen Qiao
Published: February 2022

We report on the application of a 1 μm solid-state passively Q-switched (PQS) laser and 1, 1.5 μm mode-locked (ML) fiber lasers based on ternary chalcogenide Ta2NiS5 saturable absorber (SA), which were successfully fabricated by liquid-phase exfoliation method (LPE). The nonlinear absorption of the Ta2NiS5-SA was characterized by 0.32 GW/cm2 and 0.25 GW/cm2 saturation intensities with 7.3% and 5.1% modulations depths at 1 μm and 1.5 μm, respectively. A PQS solid-state laser operating at 1.0 μm has been realized with the Ta2NiS5-SA. The maximum average output power, shortest pulse width, pulse energy, and pulse peak power from the PQS laser are 0.257 W, 180 ns, 1.265 μJ, and 7 W. Moreover, highly stable femtosecond laser centered at 1.5 μm, and picosecond centered at 1 μm, ML fiber lasers were obtained using the Ta2NiS5-SA. A 70 dB signal-to-noise ML laser with a pulse duration of 781 fs was observed in the telecommunication window, which is better than the duration of the previously reported lasers based on Ta2NiS5. The corresponding maximum single pulse energy and peak power are 0.977 nJ and 1251 W, respectively. The Ta2NiS5-SA fabricated by the LPE method was applied in near-infrared (NIR) ML fiber lasers (evanescent field interactions) and PQS bulk lasers. The results indicate that Ta2NiS5-SA prepared by the LPE method can be applied in a 1 μm bulk PQS laser and improved by the new combination mode (evanescent field interactions) for better output performance of the fiber laser.
SnO2 Anchored in S and N Co-Doped Carbon as the Anode for Long-Life Lithium-Ion Batteries
Authors: Zhou Shuli , Zhou Hongyan , Zhang Yunpeng , Zhu Keke , Zhai Yanjun , Wei Denghu , Zeng Suyuan
Published: February 2022

Tin dioxide (SnO2) has been the focus of attention in recent years owing to its high theoretical capacity (1494 mAh g−1). However, the application of SnO2 has been greatly restricted because of the huge volume change during charge/discharge process and poor electrical conductivity. In this paper, a composite material composed of SnO2 and S, N co-doped carbon (SnO2@SNC) was prepared by a simple solid-state reaction. The as-prepared SnO2@SNC composite structures show enhanced lithium storage capacity as compared to pristine SnO2. Even after cycling for 1000 times, the as-synthesized SnO2@SNC can still deliver a discharge capacity of 600 mAh g−1 (current density: 2 A g−1). The improved electrochemical performance could be attributed to the enhanced electric conductivity of the electrode. The introduction of carbon could effectively improve the reversibility of the reaction, which will suppress the capacity fading resulting from the conversion process.
Laser-Induced Morphological and Structural Changes of Cesium Lead Bromide Nanocrystals
Authors: Kostopoulou Athanasia , Brintakis Konstantinos , Sygletou Maria , Savva Kyriaki , Livakas Nikolaos , Pantelaiou Michaila Akathi , Dang Zhiya , Lappas Alexandros , Manna Liberato , Stratakis Emmanuel
Published: February 2022

Metal halide perovskite nanocrystals, an emerging class of materials for advanced photonic and optoelectronic applications, are mainly fabricated with colloidal chemistry routes. On the quest for new properties according to application needs, new perovskite systems of various morphologies and levels of doping and alloying have been developed, often also involving post-synthesis reactions. Recently, laser irradiation in liquids has been utilized as a fast method to synthesize or transform materials and interesting laser-induced transformations on nanocrystals were induced. These studies in general have been limited to small nanocrystals (~15 nm). In the case of halide perovskites, fragmentation or anion exchange have been observed in such laser-based processes, but no crystal structure transformations were actually observed or deliberately studied. Nanocrystals are more sensitive to light exposure compared to the corresponding bulk crystals. Additional factors, such as size, morphology, the presence of impurities, and others, can intricately affect the photon absorption and heat dissipation in nanocrystal suspensions during laser irradiation. All these factors can play an important role in the final morphologies and in the time required for these transformations to unfold. In the present work, we have employed a 513 nm femtosecond (fs) laser to induce different transformations in large nanocrystals, in which two phases coexist in the same particle (Cs4PbBr6/CsPbBr3 nanohexagons of ~100 nm), dispersed in dichlorobenzene. These transformations include: (i) the exfoliation of the primary nanohexagons and partial anion exchange; (ii) fragmentation in smaller nanocubes and partial anion exchange; (iii) side-by-side-oriented attachment, fusion, and formation of nanoplatelets and complete anion exchange; (iv) side-by-side attachment, fusion, and formation of nanosheets. Partial or complete Br-Cl anion exchange in the above transformations was triggered by the partial degradation of dichlorobenzene. In addition to the detailed analysis of the various nanocrystal morphologies observed in the various transformations, the structure–photoluminescence relationships for the different samples were analyzed and discussed.
The Composition and Anti-Aging Activities of Polyphenol Extract from Phyllanthus emblica L. Fruit
Authors: Wu Min , Cai Jianhang , Fang Zhengfeng , Li Shanshan , Huang Zhiqing , Tang Zizhong , Luo Qingying , Chen Hong
Published: February 2022

Phyllanthus emblica L. (PE) is commonly known as a medicine and food homologous plant, which is abundant in natural products polyphenols. In the present study, polyphenols were extracted from PE fruit by response surface method, and the anti-aging ability was determined. PE fruit polyphenols exhibited strong antioxidant capacities in scavenging free radicals, and anti-cholinesterase ability by inhibition of AChE (IC50 0.2186 ± 0.0416 mg/mL) and BuChE (IC50 0.0542 ± 0.0054 mg/mL) in vitro. Moreover, PE fruit polyphenols showed strong protective effect against the aging process in Caenorhabditis elegans model, including increased thermal resistance, extended lifespan by 18.53% (p < 0.05), reduced activity of AChE by 34.71% and BuChE by 45.38% (p < 0.01). This was accompanied by the enhancement in antioxidant enzymes activity of SOD by 30.74% (p < 0.05) and CAT by 8.42% (p > 0.05), while decrease in MDA level by 36.25% (p < 0.05). These properties might be interrelated with the presence of abundant flavonols and phenolic acids identified by UPLC-ESI-QTOF-MS, such as quercetin, myricetin, ellagic, gallic, and chlorogenic acids, together with their glycosides. The remarkable antioxidant and anti-aging potential of PE fruit polyphenols could be implemented in the food and pharmaceutical industry.
Maternal Hemoglobin Concentrations and Birth Weight, Low Birth Weight (LBW), and Small for Gestational Age (SGA): Findings from a Prospective Study in Northwest China
Authors: Liu Danmeng , Li Shanshan , Zhang Binyan , Kang Yijun , Cheng Yue , Zeng Lingxia , Chen Fangyao , Mi Baibing , Qu Pengfei , Zhao Doudou , Zhu Zhonghai , Yan Hong , Wang Duolao , Dang Shaonong
Published: February 2022

Birth weight and related outcomes have profound influences on life cycle health, but the effect of maternal hemoglobin concentration during pregnancy on birth weight is still unclear. This study aims to reveal the associations between maternal hemoglobin concentrations in different trimesters of pregnancy and neonatal birth weight, LBW, and SGA. This was a prospective study based on a cluster-randomized controlled trial conducted from July 2015 to December 2019 in rural areas of Northwest China. Information on maternal socio-demographic status, health-related factors, antenatal visits, and neonatal birth outcomes were collected. A total of 3748 women and their babies were included in the final analysis. A total of 65.1% and 46.3% of the participants had anemia or hemoglobin ≥ 130 g/L during pregnancy. In the third trimester, maternal hemoglobin concentration was associated with birth weight in an inverted U-shaped curve and with the risks of LBW and SGA in extended U-shaped curves. The relatively higher birth weight and lower risks for LBW and SGA were observed when hemoglobin concentration was 100–110 g/L. When maternal hemoglobin was <70 g/L or >130 g/L, the neonatal birth weight was more than 100 g lower than that when the maternal hemoglobin was 100 g/L. In conclusion, both low and high hemoglobin concentrations in the third trimester could be adverse to fetal weight growth and increase the risks of LBW and SGA, respectively. In addition to severe anemia, maternal hemoglobin >130 g/L in the third trimester should be paid great attention to in the practice of maternal and child health care.
Potential Roles and Key Mechanisms of Hawthorn Extract against Various Liver Diseases
Authors: Kim Eujin , Jang Eungyeong , Lee Jang-Hoon
Published: February 2022

The genus Crataegus (hawthorn), a flowering shrub or tree, is a member of the Rosaceae family and consists of approximately 280 species that have been primarily cultivated in East Asia, North America, and Europe. Consumption of hawthorn preparations has been chiefly associated with pharmacological benefits for cardiovascular diseases, including congestive heart failure and angina pectoris. Treatment with hawthorn extracts can be related to improvements in the complex pathogenesis of various hepatic and cardiovascular disorders. In this regard, the present review described that the presence of hawthorn extracts ameliorated hepatic injury, lipid accumulation, inflammation, fibrosis, and cancer in an abundance of experimental models. Hawthorn extracts might have these promising activities, largely by enhancing the hepatic antioxidant system. In addition, several mechanisms, including AMP-activated protein kinase (AMPK) signaling and apoptosis, are responsible for the role of hawthorn extracts in repairing the dysfunction of injured hepatocytes. Specifically, hawthorn possesses a wide range of biological actions relevant to the treatment of toxic hepatitis, alcoholic liver disease, non-alcoholic fatty liver disease, and hepatocellular carcinoma. Accordingly, hawthorn extracts can be developed as a major source of therapeutic agents for liver diseases.
Lutein and Zeaxanthin Intake during Pregnancy and Visual Function in Offspring at 11–12 Years of Age
Authors: Anderson Martin J. , Romaguera Dora , Saint-Amour Dave , Fossati Serena , Fochs Silvia , Pey Nuria , Vrijheid Martine , Julvez Jordi
Published: February 2022

(1) Background: Lutein and zeaxanthin (L&Z) are essential dietary nutrients that are a crucial component of the human macula, contributing to visual functioning. They easily cross the placental barrier, so that retinal deposition commences during foetal development. This study aims to assess associations between maternal L&Z intake during pregnancy and offspring visual function at 11–12 years. (2) Methods: Using the Spanish INfancia y Medio Ambiente project (INMA) Sabadell birth cohort, 431 mother–child pairs were analysed. L&Z data were obtained from food frequency questionnaires (FFQ) at week 12 and 32 of pregnancy, alongside other nutritional and sociodemographic covariates. Contrast vision (CS) and visual acuity (VA) were assessed using the automated Freiburg Acuity and Contrast Testing (FRACT) battery. Low CS and VA were defined as being below the 20th cohort centile. Associations were explored using multiple logistic regression. (3) Results: After controlling for potential confounders, L&Z intake during the 1st and 3rd trimester did not reveal any statistically significant association with either CS or VA in offspring at age 11/12 years. (4) Conclusions: No evidence of a long-term association between L&Z intake during pregnancy and visual function in offspring was found. Further larger long-term studies including blood L&Z levels are required to confirm this result.
Applying Zinc Nutrient Reference Values as Proposed by Different Authorities Results in Large Differences in the Estimated Prevalence of Inadequate Zinc Intake by Young Children and Women and in Cameroon
Authors: Haile Demewoz , Brown Kenneth H. , McDonald Christine M. , Luo Hanqi , Jarvis Michael , Teta Ismael , Ndjebayi Alex , Martial Guintang Assiene Jules , Vosti Stephen A. , Engle-Stone Reina
Published: February 2022

Nutrient reference values (NRVs) for zinc set by several expert groups differ widely and may affect the predicted prevalence of inadequate zinc intake. We examined this possibility using NRVs published by four different authorities and nationally representative dietary intake data collected among children aged 12–59 months and women in Cameroon. Usual zinc intake was estimated from 24 h recall data using the National Cancer Institute method. Prevalences of total zinc intake below the dietary requirement and of “absorbable zinc intake” below the physiological requirement were estimated using NRVs published by the World Health Organization (WHO), US Institute of Medicine (IOM), International Zinc Nutrition Consultative Group (IZiNCG), and European Food Safety Authority (EFSA). The prevalence of inadequate zinc intake ranged from 10% (IZiNCG—physiological requirement, 95% CI 7–13%) to 81% (EFSA—physiological requirement, 95% CI 78–84%) among children and 9% (WHO—physiological requirement, 95% CI 8–11.0%) to 94% (IOM—physiological requirement, 95% CI 92–95%) among women These differences in the prevalence of inadequate intake translated into sizeable differences in the predicted benefit and cost-effectiveness of zinc fortification programs. Depending on the NRVs applied, assessments differ regarding the need for and design of zinc fortification programs. Efforts are needed to harmonize NRVs for zinc.
Effect of Dietary Glycemic Index on β-Cell Function in Prediabetes: A Randomized Controlled Feeding Study
Authors: Sipe Alexander T. , Neuhouser Marian L. , Breymeyer Kara L. , Utzschneider Kristina M.
Published: February 2022

The glycemic index (GI) reflects the relative ability of carbohydrates to raise blood glucose. We utilized a controlled feeding study to assess the impact of the dietary GI on β-cell function in adults with prediabetes (17F/18M, mean ± SEM: BMI 32.44 ± 0.94 kg/m2, age 54.2 ± 1.57 years). Following a 2 week Control diet (GI = 55–58), participants were randomized to either a 4 week low GI (LGI: GI < 35, n = 17) or high GI (HGI: GI > 70, n = 18) diet (55% of energy from carbohydrate/30% fat/15% protein). The data from 4 h meal tolerance tests (MTTs) underwent mathematical modeling to assess insulin sensitivity, insulin secretion and β-cell function. Glucose concentrations during the MTT decreased on the LGI diet (p < 0.001) and trended to increase on the HGI diet (p = 0.14; LGI vs. HGI p < 0.001), with parallel changes in insulin and C-peptide concentrations. Total insulin secretion, adjusted for glucose and insulin sensitivity, increased on the LGI diet (p = 0.002), and trended lower on the HGI diet (p = 0.10; LGI vs. HGI p = 0.001). There was no significant diet effect on insulin sensitivity or other measures of β-cell function. Total insulin clearance increased on the LGI diet (p = 0.01; LGI vs. HGI p < 0.001). We conclude that short-term consumption of an LGI diet reduced glucose exposure and insulin secretion but had no impact on measures of β-cell function.
Association between Maternal Serum 25-Hydroxyvitamin D Concentrations and the Risk of Preterm Birth in Central Sudan: A Case–Control Study
Authors: Abdelrahiem Somia K. , Sharif Manal E. , ALhabardi Nadiah , Al-Wutayd Osama , Adam Ishag
Published: February 2022

There are few published studies on the association between vitamin D concentrations and preterm birth (PB) in sub-Saharan Africa. The current study aimed to assess the association between 25-hydroxyvitamin D (25[OH)] D) levels and PB. A matched case–control study (60 women in each arm) was conducted in Medani maternity hospital in central Sudan. The cases were women with spontaneous PB, and healthy women with term deliveries were the controls. The clinical/medical and obstetric history was gathered using a questionnaire. The enzyme-linked immunosorbent assay was used to measure the serum 25(OH)D levels. Women with PB had significantly lower median (interquartile range) 25(OH)D concentrations compared with the controls (18.4 (7.3) ng/mL vs. 20.2 (16.5) ng/mL, p = 0.001). Forty-two (70.0%) women with PB and 29 (48.3%) women in the control group had vitamin D deficiency (25(OH)D level ≤ 20 ng/mL). The results of the multivariable logistic regression showed that the 25(OH)D concentrations were negatively associated with PB (adjusted odds ratio (aOR) = 0.92, 95% confidence interval (CI) = 0.87–0.97). Vitamin D-deficient pregnant women were at a higher risk of PB (aOR = 2.69, 95% CI = 1.17–6.23). Low 25(OH)D concentrations were found at the time the variable was determined in women with spontaneous PB and were an independent risk factor for PB.
Effect of a Four-Week Vegan Diet on Performance, Training Efficiency and Blood Biochemical Indices in CrossFit-Trained Participants
Authors: Durkalec-Michalski Krzysztof , Domagalski Adrian , Główka Natalia , Kamińska Joanna , Szymczak Damian , Podgórski Tomasz
Published: February 2022

This interventional study examined the effect of a four-week vegan diet (VegD) during a four-week high-intensity functional training (HIFT) on performance, training results and blood biochemical indices in female (n = 12) and male (n = 8) moderate-trained CrossFit participants. The whole study group performed the maximum number of repetitions with a load of 70% one repetition-maximum (1RM) and a modified Fight Gone Bad (FGBMod) test before and after a dietary intervention (the group was divided to follow a VegD or a traditional mixed diet (MixD)) in a randomised and parallel design. Pre-exercise resting blood samples were also analysed. There was a significant improvement in the number of repetitions performed at a load corresponding to 70% of 1RM in the classic squat in the MixD group (p < 0.001), and in the classic deadlift in the VegD group (p = 0.014). Furthermore, there was a significant improvement in the results of the FGBMod performance test after a MixD. Moreover, an improvement in some exercises in the modified FGBMod test (Wall Ball after the VegD and the MixD, and rowing after the MixD) was also observed. However, differences between the MixD and the VegD groups were not clinically relevant. In conclusion, the short-term study conducted here indicated that a VegD in HIFT training positively affects strength endurance in the classic deadlift but is unlikely to be more beneficial in improving performance than a MixD.
Diet Quality Scores and Cardiometabolic Risk Factors in Mexican Children and Adolescents: A Longitudinal Analysis
Authors: Aljahdali Abeer Ali , Peterson Karen E. , Cantoral Alejandra , Ruiz-Narvaez Edward , Tellez-Rojo Martha M. , Kim Hyungjin Myra , Hébert James R. , Wirth Michael D. , Torres-Olascoaga Libni A. , Shivappa Nitin , Baylin Ana
Published: February 2022

There is limited evidence for the effects of diet on cardiometabolic profiles during the pubertal transition. We collected repeated measures of diet quality and cardiometabolic risk factors among Mexican youth. This analysis included 574 offspring of the Early Life Exposure in Mexico to Environmental Toxicants (ELEMENT) birth cohort followed up to three time points. Dietary Approaches to Stop Hypertension (DASH), alternate Mediterranean Diet (aMedDiet), and Children’s Dietary Inflammatory Index (C-DIITM) scores were computed from food frequency questionnaires. Higher DASH and aMedDiet scores reflect a higher diet quality, and lower C-DII scores reflect an anti-inflammatory diet. Cardiometabolic risk factors were lipid profile, glucose homeostasis, blood pressure, and waist circumference. Linear mixed models were used between quartiles of each diet score and outcomes. Compared to the first quartile, the fourth DASH quartile was inversely associated with log serum insulin (μIU/mL) [β = −0.19, p = 0.0034] and log-Homeostatic Model Assessment of Insulin Resistance [β = −0.25, p = 0.0008]. Additionally, log serum triglycerides (mg/dL) was linearly associated with aMedDiet score [β = −0.03, p = 0.0022]. Boys in the highest aMedDiet quartile had higher serum high-density lipoprotein cholesterol (mg/dL) [β = 4.13, p = 0.0034] compared to the reference quartile. Higher diet quality was associated with a better cardiometabolic profile among Mexican youth.
Tumor Cell Plasticity in Equine Papillomavirus-Positive Versus-Negative Squamous Cell Carcinoma of the Head and Neck
Authors: Strohmayer Carina , Klang Andrea , Kummer Stefan , Walter Ingrid , Jindra Christoph , Weissenbacher-Lang Christiane , Redmer Torben , Kneissl Sibylle , Brandt Sabine
Published: February 2022

Squamous cell carcinoma of the head and neck (HNSCC) is a common malignant tumor in humans and animals. In humans, papillomavirus (PV)-induced HNSCCs have a better prognosis than papillomavirus-unrelated HNSCCs. The ability of tumor cells to switch from epithelial to mesenchymal, endothelial, or therapy-resistant stem-cell-like phenotypes promotes disease progression and metastasis. In equine HNSCC, PV-association and tumor cell phenotype switching are poorly understood. We screened 49 equine HNSCCs for equine PV (EcPV) type 2, 3 and 5 infection. Subsequently, PV-positive versus -negative lesions were analyzed for expression of selected epithelial (keratins, β-catenin), mesenchymal (vimentin), endothelial (COX-2), and stem-cell markers (CD271, CD44) by immunohistochemistry (IHC) and immunofluorescence (IF; keratins/vimentin, CD44/CD271 double-staining) to address tumor cell plasticity in relation to PV infection. Only EcPV2 PCR scored positive for 11/49 equine HNSCCs. IHC and IF from 11 EcPV2-positive and 11 EcPV2-negative tumors revealed epithelial-to-mesenchymal transition events, with vimentin-positive cells ranging between <10 and >50%. CD44- and CD271-staining disclosed the intralesional presence of infiltrative tumor cell fronts and double-positive tumor cell subsets independently of the PV infection status. Our findings are indicative of (partial) epithelial–mesenchymal transition events giving rise to hybrid epithelial/mesenchymal and stem-cell-like tumor cell phenotypes in equine HNSCCs and suggest CD44 and CD271 as potential malignancy markers that merit to be further explored in the horse.
The Transdermal Delivery of Therapeutic Cannabinoids
Authors: Mahmoudinoodezh Haleh , Telukutla Srinivasa Reddy , Bhangu Sukhvir Kaur , Bachari Ava , Cavalieri Francesca , Mantri Nitin
Published: February 2022

Recently, several studies have indicated an increased interest in the scientific community regarding the application of Cannabis sativa plants, and their extracts, for medicinal purposes. This plant of enormous medicinal potential has been legalised in an increasing number of countries globally. Due to the recent changes in therapeutic and recreational legislation, cannabis and cannabinoids are now frequently permitted for use in clinical settings. However, with their highly lipophilic features and very low aqueous solubility, cannabinoids are prone to degradation, specifically in solution, as they are light-, temperature-, and auto-oxidation-sensitive. Thus, plant-derived cannabinoids have been developed for oral, nasal-inhalation, intranasal, mucosal (sublingual and buccal), transcutaneous (transdermal), local (topical), and parenteral deliveries. Among these administrations routes, topical and transdermal products usually have a higher bioavailability rate with a prolonged steady-state plasma concentration. Additionally, these administrations have the potential to eliminate the psychotropic impacts of the drug by its diffusion into a nonreactive, dead stratum corneum. This modality avoids oral administration and, thus, the first-pass metabolism, leading to constant cannabinoid plasma levels. This review article investigates the practicality of delivering therapeutic cannabinoids via skin in accordance with existing literature.
Free and Open-Source Posologyr Software for Bayesian Dose Individualization: An Extensive Validation on Simulated Data
Authors: Leven Cyril , Coste Anne , Mané Camille
Published: February 2022

Model-informed precision dosing is being increasingly used to improve therapeutic drug monitoring. To meet this need, several tools have been developed, but open-source software remains uncommon. Posologyr is a free and open-source R package developed to enable Bayesian individual parameter estimation and dose individualization. Before using it for clinical practice, performance validation is mandatory. The estimation functions implemented in posologyr were benchmarked against reference software products on a wide variety of models and pharmacokinetic profiles: 35 population pharmacokinetic models, with 4.000 simulated subjects by model. Maximum A Posteriori (MAP) estimates were compared to NONMEM post hoc estimates, and full posterior distributions were compared to Monolix conditional distribution estimates. The performance of MAP estimation was excellent in 98.7% of the cases. Considering the full posterior distributions of individual parameters, the bias on dosage adjustment proposals was acceptable in 97% of cases with a median bias of 0.65%. These results confirmed the ability of posologyr to serve as a basis for the development of future Bayesian dose individualization tools.
Polymer Conjugates of Antimicrobial Peptides (AMPs) with d-Amino Acids (d-aa): State of the Art and Future Opportunities
Authors: Bellotto Ottavia , Semeraro Sabrina , Bandiera Antonella , Tramer Federica , Pavan Nicola , Marchesan Silvia
Published: February 2022

In recent years, antimicrobial peptides (AMPs) have enjoyed a renaissance, as the world is currently facing an emergency in terms of severe infections that evade antibiotics’ treatment. This is due to the increasing emergence and spread of resistance mechanisms. Covalent conjugation with polymers is an interesting strategy to modulate the pharmacokinetic profile of AMPs and enhance their biocompatibility profile. It can also be an effective approach to develop active coatings for medical implants and devices, and to avoid biofilm formation on their surface. In this concise review, we focus on the last 5 years’ progress in this area, pertaining in particular to AMPs that contain d-amino acids, as well as their role, and the advantages that may arise from their introduction into AMPs.
Cutaneous Delivery of Cosmeceutical Peptides Enhanced by Picosecond- and Nanosecond-Domain Nd:YAG Lasers with Quick Recovery of the Skin Barrier Function: Comparison with Microsecond-Domain Ablative Lasers
Authors: Lee Woan-Ruoh , Hsiao Chien-Yu , Chang Zi-Yu , Wang Pei-Wen , Aljuffali Ibrahim A. , Lin Jie-Yu , Fang Jia-You
Published: February 2022

Picosecond or nanosecond-domain non-ablative lasers generate faster photothermal effects and cause less injury than microsecond lasers. In this study, we investigated the enhancing effect of 1064 nm picosecond- and nanosecond-domain neodymium (Nd):yttrium–aluminum–garnet (YAG) lasers on the cutaneous delivery of cosmeceutical peptides. Microsecond-domain fractional ablative CO2 and fully ablative erbium (Er):YAG lasers were also used for comparison. In the Franz diffusion cell study, pig or mouse skin was treated with a laser before exposure to palmitoyl tripeptide (PT)-1, PT-38, and copper tripeptide (CT)-1 at a concentration of 150 μM. Psoriasiform, atopic dermatitis (AD)-like, and photoaged skins were also developed as permeation barriers. The non-ablative laser elicited the ultrastructural disruption of the stratum corneum and epidermal vacuolation. All laser modalities significantly increased the skin permeation of peptides in vitro. The non-ablative laser chiefly enhanced peptide delivery to the receptor compartment, whereas the ablative laser mainly increased the intracutaneous peptide deposition. The picosecond- and nanosecond-domain Nd:YAG lasers elevated the amount of PT-1 in the receptor up to 40- and 22-fold compared with untreated skin, respectively. Laser treatment promoted peptide delivery in barrier-deficient and inflamed skins, although this enhancement effect was less than that observed in healthy skin. Fluorescence microscopy indicated the capability of the non-ablative laser to deliver peptides to deeper skin strata. The ablative laser confined the peptide distribution in the epidermis. Confocal microscopy showed that peptides penetrated the skin along the microdots created by the fractional Nd:YAG and CO2 lasers. The skin barrier function determined by transepidermal water loss suggested quick recovery when using a nanosecond-domain laser (within 4 h). A longer period was needed for the skin treated with the fully ablative Er:YAG laser (76−84 h). Nanosecond non-ablative laser-facilitated peptide delivery may become an efficient and safe approach for cosmeceutical applications.
Two Polarization Comb Dynamics in VCSELs Subject to Optical Injection
Authors: Doumbia Yaya , Wolfersberger Delphine , Panajotov Krassimir , Sciamanna Marc
Published: February 2022

Optical frequency comb technologies have received intense attention due to their numerous promising applications ranging from optical communications to optical comb spectroscopy. In this study, we experimentally demonstrate a new approach of broadband comb generation based on the polarization mode competition in single-mode VCSELs. More specifically, we analyze nonlinear dynamics and polarization properties in VCSELs when subject of optical injection from a frequency comb. When varying injection parameters (injection strength and detuning frequency) and comb properties (comb spacing), we unveil several bifurcation sequences enabling the excitation of free-running depressed polarization mode. Interestingly, for some injection parameters, the polarization mode competition induces a single or a two polarization comb with controllable properties (repetition rate and power per line). We also show that the performance of the two polarization combs depends crucially on the injection current and on the injected comb spacing. We explain our experimental findings by utilizing the spin-flip VCSEL model (SFM) supplemented with terms for parallel optical injection of frequency comb. We provide a comparison between parallel and orthogonal optical injection in the VCSEL when varying injection parameters and SFM parameters. We show that orthogonal comb dynamics can be observed in a wide range of parameters, as for example dichroism linear dichroism (γa=−0.1 ns−1 to γa=−0.8 ns−1), injection current (μ=2.29 to μ=5.29) and spin-flip relaxation rate (γs=50 ns−1 to γs=2300 ns−1).
Optical Switch Based on Ge2Sb2Se4Te1-Assisted Racetrack Microring
Authors: Xu Yan , Liu Songyue , Liu Tingyu , Gao Yang , Yin Yuexin , Sun Xiaoqiang , Zhang Daming
Published: February 2022

In this work, we have proposed and designed a 1 × 1 optical switch based on the optical phase-change material, Ge2Sb2Se4Te1 (GSST), for GSST-assisted silicon racetrack microring. Its optical power can periodically be exchanged between the straight silicon waveguide and the GSST/Si hybrid racetrack waveguide due to the formed directional coupling structure. By changing GSST from the crystalline state to the amorphous state, the switch shifts from the ON state to the OFF state, and vice versa. With finite-difference time-domain method optimization, the proposed switch shows an extinction ratio of 18 dB at 1547.4 nm. The insert losses at the ON and OFF states are both less than 1 dB. The proposed switch unit has the potential to build an N × N switch matrix.
Halogens in Seaweeds: Biological and Environmental Significance
Authors: Al-Adilah Hanan , Feiters Martin C. , Carpenter Lucy J. , Kumari Puja , Carrano Carl J. , Al-Bader Dhia , Küpper Frithjof C.
Published: February 2022

Many marine algae are strong accumulators of halogens. Commercial iodine production started by burning seaweeds in the 19th century. The high iodine content of certain seaweeds has potential pharmaceutical and nutritional applications. While the metabolism of iodine in brown algae is linked to oxidative metabolism, with iodide serving the function of an inorganic antioxidant protecting the cell and thallus surface against reactive oxygen species with implications for atmospheric and marine chemistry, rather little is known about the regulation and homoeostasis of other halogens in seaweeds in general and the ecological and biological role of marine algal halogenated metabolites (except for organohalogen secondary metabolites). The present review covers these areas, including the significance of seaweed-derived halogens and of halogens in general in the context of human diet and physiology. Furthermore, the understanding of interactions between halogenated compound production by algae and the environment, including anthropogenic impacts, effects on the ozone layer and global climate change, is reviewed together with the production of halogenated natural products by seaweeds and the potential of seaweeds as bioindicators for halogen radionuclides.