bluuebunny/crossref_metadata_2025 huggingface dataset:
Degradation of Chlorsulfuron by Soil Microorganisms
Authors: Joshi Madan M. , Brown Hugh M. , Romesser James A.
Published: January 2018

Degradation of chlorsulfuron {2-chloro-N-[[(4-methoxy-6-methyl-1,3,5-triazin-yl)amino] carbonyl] benzenesulfonamide} in acidic and alkaline soils was evaluated using plant bioassay and high-performance liquid chromatography (HPLC) radiotracer techniques. Soil sterilization with either ethylene oxide (Et0) or gamma irradiation significantly reduced breakdown of chlorsulfuron; the ability for degradation was restored by reinoculation with indigenous soil microorganisms. Streptomyces griseolus (a soil actinomycete), Aspergillus niger, and Penicillium sp. (soil fungi) were demonstrated to degrade 14C-chlorsulfuron in pure culture. In addition to microbial breakdown, chemical hydrolysis was an important factor in the disappearance of chlorsulfuron from soil. The contribution of chemical hydrolysis to total degradation was a function of soil pH, with hydrolysis occurring most rapidly in acidic soils. Both dissipation processes slowed markedly at low temperatures.
Colletotrichum coccodes: A Pathogen of Eastern Black Nightshade (Solanum ptycanthum)
Authors: Andersen Robert N. , Walker H. Lynn
Published: January 2018

The fungusColletotrichum coccodes(Wallr.) Hughes was isolated from diseased plants of eastern black nightshade (Solanum ptycanthumDun. ♯ SOLPT) growing in the field. In greenhouse experiments, the isolate was highly pathogenic to eastern black nightshade and less so to seven other weedy species within the Solanaceae. No disease symptoms were observed on seedlings of tomato (Lycopersicon esculentumMill.), potato (Solanum tuberosumL.), or 10 other species inoculated with the isolate. Dew periods of 16 h or longer were required for maximum disease development. This requirement could limit the practical use of this pathogen as a my coherbicide.
Selection of Herbicide Alternatives Based on Probable Leaching to Groundwater
Authors: Franklin Ralph E. , Quisenberry Virgil L. , Gossett Billy J. , Murdock Edward C.
Published: January 2018

Extension workers are sensing pressure to use soils information and chemical characteristics data to guide farmers in selecting pesticides least prone to leach into groundwater. Our objective was to estimate differences in herbicide migration to groundwater under conditions typical for the Southeast Coastal Plain, and to consider how a farmer might be advised to use such knowledge in selecting herbicides. We used a simple computer code for microcomputers to predict persistence and migration of 17 herbicides through a hypothetical, coarse-textured soil typical of the Southeast Coastal Plain. Appropriate herbicides were selected for several common crop-weed problems, such as sicklepod in soybean and Palmer amaranth in corn. Groundwater was assumed to be 3.15 m below the soil surface. Herbicides selected covered a broad range of half-lives and organic carbon partition coefficients. Only after the first-order degradation rate constant was reduced by a factor of five did predicted soil water concentrations of several herbicides at the groundwater interface reach normal detection limits. Still, predicted concentrations were below the level established for health effects advisory purposes. Due to the large number of uncertainties and the inability to estimate practical benefits, we conclude that data relating to soil and herbicide characteristics cannot be used at this time to override cost effectiveness, efficacy, and other factors normally considered by farmers and Extension professionals in herbicides for weed control.
Bermudagrass (Cynodon dactylon) Sod Rooting as Influenced by Preemergence Herbicides
Authors: Fishel Frederick M. , Coats G. Euel
Published: January 2018

The influence of PRE herbicides on rooting characteristics of bermudagrass turfgrass was studied using sod plugs removed from the field. Greenhouse root fresh weight was reduced the greatest with dithiopyr or prodiamine. Pendimethalin caused fewer fresh weight reductions compared to dithiopyr or prodiamine and these reductions were usually for a short period. Root weight loss was due to lower root density and root malformation. Significant numbers of malformed roots were also observed for greater periods of time where dithiopyr or prodiamine had been applied.
Comparison of Herbicide Programs and Cultivation for Sicklepod (Cassia obtusifolia) Control in Soybean (Glycine max)
Authors: Miller Donnie K. , Griffin James L.
Published: January 2018

Sicklepod control mid-season in cultivated soybean was higher when imazaquin plus AC 263,222 or AC 263,222 alone was applied POST following pendimethalin plus imazaquin PPI compared with only the PPI treatment. Pendimethalin plus imazaquin PPI followed by imazaquin plus AC 263,222 at 36 + 18 g ai/ha or followed by AC 263,222 at 18 g ai/ha with cultivation controlled sicklepod equal to pendimethalin PPI followed by imazaquin plus AC 263,222 at 71 + 18 g ai/ha or followed by a sequential application of AC 263,222 at 13 g ai/ha early POST followed by 9 g ai/ha late POST. Control with these treatments was no higher than for the standard of pendimethalin plus metribuzin PPI followed by chlorimuron POST at 9 g ai/ha, which controlled 86% sicklepod. Averaged across herbicide treatments, cultivation reduced percent sicklepod seed in harvested soybean samples 33% and increased soybean yield 19% compared with no cultivation. Soybean yield was highest for the chlorimuron standard and greater than that for the other treatments when averaged across cultivation treatments.
A Simple Low Volume Laboratory Research Spray System
Authors: Campbell Robert A. , Wood James E. , Mitchell E. Garth , Studens John , Wagner Robert G.
Published: January 2018

Commercially available cabinet sprayers are not well suited for making low volume applications (<30 L/ha) of herbicides to woody forest species that can be up to 1.5 m tall. A simple, inexpensive laboratory sprayer that overcomes some limitations of commercial cabinet sprays can be built from materials readily available at local building and electronic suppliers. The only specialized equipment required is a positive displacement pump and a rotary disk atomizer. The atomizer is attached to the end of a variable height arm mounted on a laboratory cart. A positive displacement pump ensures controlled flow. The operator pushes the cart along a metal rub rail which keeps the cart tracking in a straight line. Travel speed is regulated by the operator following a marker on a clothesline-like loop of fishing line and is driven by a variable-speed drill attached to a variable voltage power supply.
Movement of14C-Bentazon with Adjuvants into Common Cocklebur (Xanthium strumarium) and Velvetleaf (Abutilon theophrasti)
Authors: Levene Brian C. , Owen Micheal D. K.
Published: January 2018

Absorption of14C-bentazon from the leaf surfaces of common cocklebur and velvetleaf was rapid and most occurred within 4 h after foliar application. Greater14C in the epicuticular wax (ECW) did not correlate with higher radioactivity in the leaf tissue. There was significantly less ECW on the “mature” leaves of common cocklebur and “juvenile” leaves of velvetleaf. However, the “mature” leaves exhibited greater14C absorption for both species. The use of 28% urea ammonium nitrate increased14C-absorption when compared with crop oil concentrate and with no adjuvant.
Competitive Abilities of Six Corn (Zea maysL.) Hybrids with Four Weed Control Practices
Authors: Ford Garry Tyron , Pleasant Jane Mt.
Published: January 2018

Six corn hybrids were studied in field experiments in 1989 and 1990 to identify hybrids and corn plant characteristics that may be valuable in systems using reduced levels of weed control. Four weed control treatments (no-control, cultivation-only, band herbicide-plus-cultivation, and broadcast herbicide) represented main plots and maize hybrids were subplots. Medium-season hybrids with differences in height, early-season vigor, and leafiness were used. There were significant differences among hybrids in leaf angle, leaf width, leaf number, plant height, leaf area index (LAI), plant dry matter (DM) and grain and stover yields. Lower yielding hybrids had grain yields that ranged from 87 to 91% of the highest yielding hybrid. Aboveground corn characteristics were not correlated with weed numbers, weed cover, or weed biomass. A significant interaction between hybrid and weed control for grain yields was observed in 1989, suggesting that some hybrids are more competitive when weed pressure is high.
Effects of Tank-Mix Combinations of Non-Selective Foliar and Selective Soil-Applied Herbicides on Three Weed Species
Authors: Hydrick David E. , Shaw David R.
Published: January 2018

Greenhouse experiments were established to investigate the effects of tank-mixing glyphosate, paraquat, or glufosinate with metribuzin plus chlorimuron, imazaquin, or metribuzin on entireleaf morningglory, sicklepod, and johnsongrass control. Antagonism was the most frequent interaction, and usually occurred when the lower rates of non-selective foliar-active herbicides were used in tank mixtures with selective soil-active herbicides. Antagonism occurred on all species when 180 g ai/ha paraquat was tank-mixed with 90 g ai/ha metribuzin plus 15 g ai/ha chlorimuron. When the rates of non-selective herbicide were increased, antagonism was usually overcome. Antagonism also occurred on entireleaf morningglory control when 210 g ai/ha glyphosate was tank-mixed with 90 g/ha metribuzin plus 15 g/ha chlorimuron or 36 g ai/ha imazaquin. When lower rates of paraquat or glufosinate were tank-mixed with 210 g/ha metribuzin, antagonism also occurred. Less antagonism was noted with glufosinate.
Sweet Potato Periderm Components Inhibit Yellow Nutsedge (Cyperus esculentus) Growth
Authors: Harrison Howard F. , Peterson Joseph K.
Published: January 2018

Sequential extraction and chromatographic procedures were used to isolate inhibitors of yellow nutsedge growth from sweet potato periderm tissue. Most of the inhibitory activity was found in a single high pressure liquid chromatography peak that contained 1.2% of the periderm dry weight. Concentration-response data indicated that yellow nutsedge is highly sensitive to this fraction. Several other fractions inhibited yellow nutsedge root growth, but they did not compose a major portion of the inhibitory capacity of the periderm extracts.
Publisher Correction: Observation of transverse spin Nernst magnetoresistance induced by thermal spin current in ferromagnet/non-magnet bilayers
Authors: Kim Dong-Jun , Jeon Chul-Yeon , Choi Jong-Guk , Wook Lee Jae , Surabhi Srivathsava , Jeong Jong-Ryul , Lee Kyung-Jin , Park Byong-Guk
Published: January 2018

The original version of this Article contained an error in ref. 27, which was incorrectly given with the wrong journal name as:

Meyer, S. et al. Observation of the spin Nernst effect. Nat. Phys. 16, 977–981 (2017).

The correct form of ref. 27 is:

Meyer, S. et al. Observation of the spin Nernst effect. Nat. Mater. 16, 977–981 (2017).

This has now been corrected in the PDF and HTML versions of the Article.
The use of accelerometry as a tool to measure disturbed nocturnal sleep in Parkinson’s disease
Authors: McGregor Sarah , Churchward Philip , Soja Katarzyna , O’Driscoll Denise , Braybrook Michelle , Khodakarami Hamid , Evans Andrew , Farzanehfar Parisa , Hamilton Garun , Horne Malcolm
Published: January 2018

Sleep disturbances are common in Parkinson’s disease (PD). We used the Parkinson’s KinetiGraph (PKG), an objective movement recording system for PD to assess night time sleep in 155 people aged over 60 and without PD (controls), 72 people with PD (PwP) and 46 subjects undergoing a Polysomnogram (PSG: 36 with sleep disorder and 10 with normal sleep). The PKG system uses a wrist worn logger to capture acceleration and derive a bradykinesia score (BKS) every 2 min over 6 days. The BKS ranges from 0–160 with higher scores associated with lesser mobility. Previously we showed that BKS > 80 were associated with day time sleep and used this to produce scores for night time sleep: Efficiency (Percent time with BKS > 80), Fragmentation (Average duration of runs of BKS > 80) and Sleep Quality (BKS > 111 as a representation of atonia). There was a fair association with BKS score and sleep level as judged by PSG. Using these PKG scores, it was possible to distinguish between normal and abnormal PSG studies with good Selectivity (86%) and Sensitivity (80%). The PKG’s sleep scores were significantly different in PD and Controls and correlated with a subject’s self-assessment (PDSS 2) of the quality, wakefulness and restlessness. Using both the PDSS 2 and the PKG, it was apparent that sleep disturbances were apparent early in disease in many PD subjects and that subjects with poor night time sleep were more likely to have day time sleepiness. This system shows promise as a quantitative score for assessing sleep in Parkinson’s disease.
Distinction of Plasmodium ovale wallikeri and Plasmodium ovale curtisi using quantitative Polymerase Chain Reaction with High Resolution Melting revelation
Authors: Joste V. , Kamaliddin C. , Kendjo E. , Hubert V. , Argy N. , Houzé S.
Published: January 2018

Plasmodium ovale curtisi (Poc) and Plasmodium ovale wallikeri (Pow) have been described as two distinct species, only distinguishable by molecular methods such as PCR. Because of no well-defined endemic area and a variable clinical presentation as higher thrombocytopenia and nausea associated with Pow infection and asymptomatic forms of the pathology with Poc infection, rapid and specific identification of Plasmodium ovale curtisi and Plasmodium ovale wallikeri are needed. The aim of the study was to evaluate a new quantitative real-time PCR coupled with high resolution melting revelation (qPCR-HRM) for identification of both species. Results were compared with a nested-PCR, considered as a gold standard for Pow and Poc distinction. 356 samples including all human Plasmodium species at various parasitaemia were tested. The qPCR-HRM assay allowed Poc and Pow discrimination in 66 samples tested with a limit of detection evaluated at 1 parasite/µL. All these results were concordant with nested-PCR. Cross-reaction was absent with others blood parasites. The qPCR-HRM is a rapid and convenient technique to Poc and Pow distinction.
Specific inhibition of acetylcholinesterase as an approach to decrease muscarinic side effects during myasthenia gravis treatment
Authors: Petrov Konstantin A. , Kharlamova Alexandra D. , Lenina Oksana A. , Nurtdinov Ayrat R. , Sitdykova Marina E. , Ilyin Victor I. , Zueva Irina V. , Nikolsky Evgeny E.
Published: January 2018

Non-selective inhibitors of cholinesterases (ChEs) are clinically used for treatment of myasthenia gravis (MG). While being generally safe, they cause numerous adverse effects including induction of hyperactivity of urinary bladder and intestines affecting quality of patients life. In this study we have compared two ChEs inhibitors, a newly synthesized compound C547 and clinically used pyridostigmine bromide, by their efficiency to reduce muscle weakness symptoms and ability to activate contractions of urinary bladder in a rat model of autoimmune MG. We found that at dose effectively reducing MG symptoms, C547 did not affect activity of rat urinary bladder. In contrast, at equipotent dose, pyridostigmine caused a significant increase in tonus and force of spontaneous contractions of bladder wall. We also found that this profile of ChEs inhibitors translates into the preparation of human urinary bladder. The difference in action observed for C547 and pyridostigmine we attribute to a high level of pharmacological selectivity of C547 in inhibiting acetylcholinesterase as compared to butyrylcholinesterase. These results raise reasonable hope that selective acetylcholinesterase inhibitors should show efficacy in treating MG in human patients with a significant reduction in adverse effects related to hyperactivation of smooth muscles.
Biopsy Needle Integrated with Electrical Impedance Sensing Microelectrode Array towards Real-time Needle Guidance and Tissue Discrimination
Authors: Park Jaeho , Choi Won-Mook , Kim Kyuyoung , Jeong Won-Il , Seo Joon-Beom , Park Inkyu
Published: January 2018

A biopsy needle with electrical impedance sensor array based on stainless steel microelectrodes (EIS needle) was developed for real-time four electrode measurement and multi-spot sensing of tissues during the biopsy process. The sensor performance was characterized by using saline solutions with various concentrations, which proved accurate, stable and reliable electrical impedance measurement. The capability of impedance-based tissue sensing was verified by the conductivity measurement of agarose hydrogel based phantom mimicking cancer tissue. Furthermore, multi-spot impedance sensing during needle insertion was demonstrated using porcine meat with muscle and fat layers, which exhibited a clear discrimination between different types of tissues. Also, the electrical impedance difference between normal and fatty livers of mouse model was measured by the EIS needle. We could successfully demonstrate that the EIS needle can provide localized and accurate characterization of biological tissues at the needle tip.
A new, large-bodied omnivorous bat (Noctilionoidea: Mystacinidae) reveals lost morphological and ecological diversity since the Miocene in New Zealand
Authors: Hand Suzanne J. , Beck Robin M. D. , Archer Michael , Simmons Nancy B. , Gunnell Gregg F. , Scofield R. Paul , Tennyson Alan J. D. , De Pietri Vanesa L. , Salisbury Steven W. , Worthy Trevor H.
Published: January 2018

A new genus and species of fossil bat is described from New Zealand’s only pre-Pleistocene Cenozoic terrestrial fauna, the early Miocene St Bathans Fauna of Central Otago, South Island. Bayesian total evidence phylogenetic analysis places this new Southern Hemisphere taxon among the burrowing bats (mystacinids) of New Zealand and Australia, although its lower dentition also resembles Africa’s endemic sucker-footed bats (myzopodids). As the first new bat genus to be added to New Zealand’s fauna in more than 150 years, it provides new insight into the original diversity of chiropterans in Australasia. It also underscores the significant decline in morphological diversity that has taken place in the highly distinctive, semi-terrestrial bat family Mystacinidae since the Miocene. This bat was relatively large, with an estimated body mass of ~40 g, and its dentition suggests it had an omnivorous diet. Its striking dental autapomorphies, including development of a large hypocone, signal a shift of diet compared with other mystacinids, and may provide evidence of an adaptive radiation in feeding strategy in this group of noctilionoid bats.
Aster leafhopper survival and reproduction, and Aster yellows transmission under static and fluctuating temperatures, using ddPCR for phytoplasma quantification
Authors: Bahar Md H. , Wist Tyler J. , Bekkaoui Diana R. , Hegedus Dwayne D. , Olivier Chrystel Y.
Published: January 2018

Aster yellows (AY) is an important disease of Brassica crops and is caused by Candidatus Phytoplasma asteris and transmitted by the insect vector, Aster leafhopper (Macrosteles quadrilineatus). Phytoplasma-infected Aster leafhoppers were incubated at various constant and fluctuating temperatures ranging from 0 to 35 °C with the reproductive host plant barley (Hordium vulgare). At 0 °C, leafhopper adults survived for 18 days, but failed to reproduce, whereas at 35 °C insects died within 18 days, but successfully reproduced before dying. Temperature fluctuation increased thermal tolerance in leafhoppers at 25 °C and increased fecundity of leafhoppers at 5 and 20 °C. Leafhopper adults successfully infected and produced AY-symptoms in canola plants after incubating for 18 days at 0–20 °C on barley, indicating that AY-phytoplasma maintains its virulence in this temperature range. The presence and number of AY-phytoplasma in insects and plants were confirmed by droplet digital PCR (ddPCR) quantification. The number of phytoplasma in leafhoppers increased over time, but did not differ among temperatures. The temperatures associated with a typical crop growing season on the Canadian Prairies will not limit the spread of AY disease by their predominant insect vector. Also, ddPCR quantification is a useful tool for early detection and accurate quantification of phytoplasma in plants and insects.
Neural measures of the role of affective prosody in empathy for pain
Authors: Meconi Federica , Doro Mattia , Schiano Lomoriello Arianna , Mastrella Giulia , Sessa Paola
Published: January 2018

Emotional communication often needs the integration of affective prosodic and semantic components from speech and the speaker’s facial expression. Affective prosody may have a special role by virtue of its dual-nature; pre-verbal on one side and accompanying semantic content on the other. This consideration led us to hypothesize that it could act transversely, encompassing a wide temporal window involving the processing of facial expressions and semantic content expressed by the speaker. This would allow powerful communication in contexts of potential urgency such as witnessing the speaker’s physical pain. Seventeen participants were shown with faces preceded by verbal reports of pain. Facial expressions, intelligibility of the semantic content of the report (i.e., participants’ mother tongue vs. fictional language) and the affective prosody of the report (neutral vs. painful) were manipulated. We monitored event-related potentials (ERPs) time-locked to the onset of the faces as a function of semantic content intelligibility and affective prosody of the verbal reports. We found that affective prosody may interact with facial expressions and semantic content in two successive temporal windows, supporting its role as a transverse communication cue.
Random telegraph noise from resonant tunnelling at low temperatures
Authors: Li Zuo , Sotto Moïse , Liu Fayong , Husain Muhammad Khaled , Yoshimoto Hiroyuki , Sasago Yoshitaka , Hisamoto Digh , Tomita Isao , Tsuchiya Yoshishige , Saito Shinichi
Published: January 2018

The Random Telegraph Noise (RTN) in an advanced Metal-Oxide-Semiconductor Field-Effect Transistor (MOSFET) is considered to be triggered by just one electron or one hole, and its importance is recognised upon the aggressive scaling. However, the detailed nature of the charge trap remains to be investigated due to the difficulty to find out the exact device, which shows the RTN feature over statistical variations. Here, we show the RTN can be observed from virtually all devices at low temperatures, and provide a methodology to enable a systematic way to identify the bias conditions to observe the RTN. We found that the RTN was observed at the verge of the Coulomb blockade in the stability diagram of a parasitic Single-Hole-Transistor (SHT), and we have successfully identified the locations of the charge traps by measuring the bias dependence of the RTN.
Uncoupled mitochondria quickly shorten along their long axis to form indented spheroids, instead of rings, in a fission-independent manner
Authors: Miyazono Yoshihiro , Hirashima Shingo , Ishihara Naotada , Kusukawa Jingo , Nakamura Kei-ichiro , Ohta Keisuke
Published: January 2018

Loss of mitochondrial membrane potential (ΔΨm) triggers dramatic structural changes in mitochondria from a tubular to globular shape, referred to as mitochondrial fragmentation; the resulting globular mitochondria are called swelled or ring/doughnut mitochondria. We evaluated the early period of structural changes during the ΔΨm loss-induced transformation after carbonyl cyanide m-chlorophenyl hydrazine (CCCP) administration using a newly developed correlative microscopic method combined with fluorescence microscopic live imaging and volume electron microscopy. We found that most mitochondria changed from a tubular shape to a globular shape without fusion or fission and typically showed ring shapes within 10 min after CCCP exposure. In contrast, most ring mitochondria did not have a true through hole; rather, they had various indents, and 47% showed stomatocyte shapes with vase-shaped cavities, which is the most stable physical structure without any structural support if the long tubular shape shortens into a sphere. Our results suggested that loss of ΔΨm triggered collapse of mitochondrial structural support mechanisms.
Prediction of co-expression genes and integrative analysis of gene microarray and proteomics profile of Keshan disease
Authors: Wang Sen , Yan Rui , Wang Bin , Du Peiru , Tan Wuhong , Lammi Mikko J. , Guo Xiong
Published: January 2018

Keshan disease (KD) is a kind of endemic cardiomyopathy which has a high mortality. However, molecular mechanism in the pathogenesis of KD remains poorly understood. Serum samples were collected from 112 KD patients and 112 normal controls. Gene microarray was used to screen differently expressed genes. Genevestigator was applied to forecast co-expression genes of significant gene. iTRAQ proteomics analysis was used to verify significant genes and their co-expression genes. GO, COG, IPA and STRING were applied to undertake function categorization, pathway and network analysis separately. We identified 32 differentially expressed genes; IDH2, FEM1A, SSPB1 and their respective 30 co-expression genes; 68 differential proteins in KD. Significant proteins were categorized into 23 biological processes, 16 molecular functions, 16 cellular components, 15 function classes, 13 KD pathways and 1 network. IDH2, FEM1A, SSBP1, CALR, NDUFS2, IDH3A, GAPDH, TCA Cycle II (Eukaryotic) pathway and NADP repair pathway may play important roles in the pathogenesis of KD.
COX-2 induces oncogenic micro RNA miR655 in human breast cancer
Authors: Majumder Mousumi , Dunn Leanna , Liu Ling , Hasan Asma , Vincent Krista , Brackstone Muriel , Hess David , Lala Peeyush K.
Published: January 2018

We show that Cyclooxygenase-2 over-expression induces an oncogenic microRNA miR655 in human breast cancer cells by activation of EP4. MiR655 expression positively correlated with COX-2 in genetically disparate breast cancer cell lines and increased in all cell lines when grown as spheroids, implicating its link with stem-like cells (SLCs). Ectopic miR655 over-expression in MCF7 and SKBR3 cells resulted in increased proliferation, migration, invasion, spheroid formation and Epithelial to Masenchymal transition (EMT). Conversely, knocking down miR655 in aggressive MCF7-COX2 and SKBR3-COX2 cells reverted these phenotypes. MCF7-miR655 cells displayed upregulated NOTCH/WNT genes; both pathway inhibitors abrogated miR655-induced spheroid formation, linking miR655 with SLC-related pathways. MiR655 expression was dependent on EP4 activity and EP4 downstream signaling pathways PI3K/AKT, ERK and NF-kB and led to TGFβ resistance for Smad3 phosphorylation. Tail vein injection of MCF7-miR655 and SKBR3-miR655 cells in NOD/SCID/GUSB-null mice revealed increased lung colony growth and micrometastases to liver and spleen. MiR655 expression was significantly high in human breast tumors (n = 105) compared to non-tumor tissues (n = 20) and associated with reduced patient survival. Thus miR655 could serve as a prognostic breast cancer biomarker.
Role of diffusion-weighted imaging in the discrimination of benign and metastatic parotid area lymph nodes in patients with nasopharyngeal carcinoma
Authors: Chen Chuanben , Lin Zhizhong , Xiao Youping , Bai Penggang , Yue Qiuyuan , Chen Yunbin , Chen Lisha
Published: January 2018

To assess the utility of apparent diffusion coefficient (ADC) determined on diffusion-weighted MR imaging (DWI) to differentiate between benign and malignant parotid area lymph nodes (PLN) in nasopharyngeal carcinoma (NPC) patients. Thirty-nine consecutive NPC patients with a total of 40 enlarged, biopsied PLNs underwent DWI examination. ADC values for benign and malignant PLNs were measured and compared. Receiver operating characteristic (ROC) curve analysis was to evaluate the optimal threshold level of ADC values for metastatic PLNs. The kappa was to assess the degree of agreement between histopathological diagnoses and ADC values, or imaging features of PLNs on MRI. We found the mean ADC value for benign PLNs was markedly higher than malignant PLNs. A threshold ADC of 1.01 × 10−3 mm2/s was associated with a sensitivity of 85.7% and a specificity of 72.7% (area under the curve: 0.84). A moderate agreement was observed between the histopathological diagnosis and the threshold of ADC value (k value: 0.483). However, short axis diameter, necrosis, extranodal extension, and regional grouping of PLNs on MRI showed only a fair agreement with the histopathological diagnosis (k value: 0.257, 0.305, 0.276, and 0.205, respectively). Therefore, DWI may be a promising technique to differentiate metastatic from benign PLNs.
Phenotypic responses to interspecies competition and commensalism in a naturally-derived microbial co-culture
Authors: Khan Nymul , Maezato Yukari , McClure Ryan S. , Brislawn Colin J. , Mobberley Jennifer M. , Isern Nancy , Chrisler William B. , Markillie Lye Meng , Barney Brett M. , Song Hyun-Seob , Nelson William C. , Bernstein Hans C.
Published: January 2018

The fundamental question of whether different microbial species will co-exist or compete in a given environment depends on context, composition and environmental constraints. Model microbial systems can yield some general principles related to this question. In this study we employed a naturally occurring co-culture composed of heterotrophic bacteria, Halomonas sp. HL-48 and Marinobacter sp. HL-58, to ask two fundamental scientific questions: 1) how do the phenotypes of two naturally co-existing species respond to partnership as compared to axenic growth? and 2) how do growth and molecular phenotypes of these species change with respect to competitive and commensal interactions? We hypothesized – and confirmed – that co-cultivation under glucose as the sole carbon source would result in competitive interactions. Similarly, when glucose was swapped with xylose, the interactions became commensal because Marinobacter HL-58 was supported by metabolites derived from Halomonas HL-48. Each species responded to partnership by changing both its growth and molecular phenotype as assayed via batch growth kinetics and global transcriptomics. These phenotypic responses depended on nutrient availability and so the environment ultimately controlled how they responded to each other. This simplified model community revealed that microbial interactions are context-specific and different environmental conditions dictate how interspecies partnerships will unfold.
Synthesis of Wurtzite Cu2ZnSnS4 Nanosheets with Exposed High-Energy (002) Facets for Fabrication of Efficient Pt-Free Solar Cell Counter Electrodes
Authors: Zhang Xiaoyan , Xu You , Zhang Junjie , Dong Shuai , Shen Liming , Gupta Arunava , Bao Ningzhong
Published: January 2018

Two-dimensional (2D) semiconducting nanomaterials have generated much interest both because of fundamental scientific interest and technological applications arising from the unique properties in two dimensions. However, the colloidal synthesis of 2D quaternary chalcogenide nanomaterials remains a great challenge owing to the lack of intrinsic driving force for its anisotropic growth. 2D wurtzite Cu2ZnSnS4 nanosheets (CZTS-NS) with high-energy (002) facets have been obtained for the first time via a simple one-pot thermal decomposition method. The CZTS-NS exhibits superior photoelectrochemical activity as compared to zero-dimensional CZTS nanospheres and comparable performance to Pt counter electrode for dye sensitized solar cells. The improved catalytic activity can be attributed to additional reactive catalytic sites and higher catalytic reactivity in high-energy (002) facets of 2D CZTS-NS. This is in accordance with the density functional theory (DFT) calculations, which indicates that the (002) facets of wurtzite CZTS-NS possess higher surface energy and exhibits remarkable reducibility for I3− ions. The developed synthetic method and findings will be helpful for the design and synthesis of 2D semiconducting nanomaterials, especially eco-friendly copper chalcogenide nanocrystals for energy harvesting and photoelectric applications.
Probiotic activities of Rhizobium laguerreae on growth and quality of spinach
Authors: Jiménez-Gómez Alejandro , Flores-Félix José David , García-Fraile Paula , Mateos Pedro F. , Menéndez Esther , Velázquez Encarna , Rivas Raúl
Published: January 2018

The growing interest in a healthy lifestyle and in environmental protection is changing habits regarding food consumption and agricultural practices. Good agricultural practice is indispensable, particularly for raw vegetables, and can include the use of plant probiotic bacteria for the purpose of biofertilization. In this work we analysed the probiotic potential of the rhizobial strain PEPV40, identified as Rhizobium laguerreae through the analysis of the recA and atpD genes, on the growth of spinach plants. This strain presents several in vitro plant growth promotion mechanisms, such as phosphate solubilisation and the production of indole acetic acid and siderophores. The strain PEPV40 produces cellulose and forms biofilms on abiotic surfaces. GFP labelling of this strain showed that PEPV40 colonizes the roots of spinach plants, forming microcolonies typical of biofilm initiation. Inoculation with this strain significantly increases several vegetative parameters such as leaf number, size and weight, as well as chlorophyll and nitrogen contents. Therefore, our findings indicate, for the first time, that Rhizobium laguerreae is an excellent plant probiotic, which increases the yield and quality of spinach, a vegetable that is increasingly being consumed raw worldwide.
Spin-coupling-induced Improper Polarizations and Latent Magnetization in Multiferroic BiFeO3
Authors: Jang Hyun Myung , Han Hyeon , Lee Jung-Hoon
Published: January 2018

Multiferroic BiFeO3 (BFO) that exhibits a gigantic off-centering polarization (OCP) is the most extensively studied material among all multiferroics. In addition to this gigantic OCP, the BFO having R3c structural symmetry is expected to exhibit a couple of parasitic improper polarizations owing to coexisting spin-polarization coupling mechanisms. However, these improper polarizations are not yet theoretically quantified. Herein, we show that there exist two distinct spin-coupling-induced improper polarizations in the R3c BFO on the basis of the Landau-Lifshitz-Ginzburg theory: ΔP
LF
arising from the Lifshitz gradient coupling in a cycloidal spin-density wave, and ΔP
ms
originating from the biquadratic magnetostrictive interaction. With the help of ab initio calculations, we have numerically evaluated magnitudes of these improper polarizations, in addition to the estimate of all three relevant coupling constants. We further predict that the magnetic susceptibility increases substantially upon the transition from the bulk R3c BFO to the homogeneous canted spin state in a constrained epitaxial film, which satisfactorily accounts for the experimental observation. The present study will help us understand the magnetoelectric coupling and shed light on design of BFO-based materials with improved multiferroic properties.
Transfer of a bla
CTX-M-1-carrying plasmid between different Escherichia coli strains within the human gut explored by whole genome sequencing analyses
Authors: Knudsen Per Kristian , Gammelsrud Karianne Wiger , Alfsnes Kristian , Steinbakk Martin , Abrahamsen Tore G. , Müller Fredrik , Bohlin Jon
Published: January 2018

Horizontal transfer of antibiotic resistance determinants contributes to dissemination of antibiotic resistance. Such transfer of resistance genes within the human gut has been documented in some in vivo studies. The present study investigated seven blaCTX-M-1-carrying Escherichia coli isolates from three consecutive faecal samples collected from one cystic fibrosis patient in a nine-months period, by analysing whole genome sequencing data. The analyses showed that the seven E. coli isolates represented three genetically diverse strains. All isolates contained blaCTX-M-1-carrying Incl1 plasmids that shared a common 101 kb backbone differing by only four SNPs. The plasmids harboured by the three different E. coli strains varied within limited regions suggestive of recombination events, according to the phylogenetic topology of the genomes of the isolates harbouring them. The findings strongly suggest that horizontal transfer of a blaCTX-M-1-carrying plasmid had occurred within the patient´s gut. The study illustrates the within-host diversity of faecally carried resistant E. coli isolates and highlights the value of collecting multiple bacterial colonies from longitudinally collected samples to assess faecal carriage of resistant enterobacteria. The clustering of the plasmids with the corresponding E. coli strains carrying them indicates that the plasmids appear to have adapted to their respective E. coli hosts.
Respiratory Syncytial Virus Infection Changes Cargo Composition of Exosome Released from Airway Epithelial Cells
Authors: Chahar Harendra Singh , Corsello Tiziana , Kudlicki Andrzej S. , Komaravelli Narayana , Casola Antonella
Published: January 2018

Exosomes are microvesicles known to carry biologically active molecules, including RNA, DNA and proteins. Viral infections can induce profound changes in exosome composition, and exosomes have been implicated in viral transmission and pathogenesis. No information is current available regarding exosome composition and function during infection with Respiratory Syncytial Virus (RSV), the most important cause of lower respiratory tract infections in children. In this study, we characterized exosomes released from RSV-infected lung carcinoma-derived A549 cells. RNA deep sequencing revealed that RSV exosomes contain a diverse range of RNA species like messenger and ribosomal RNA fragments, as well as small noncoding RNAs, in a proportion different from exosomes isolated from mock-infected cells. We observed that both RNA and protein signatures of RSV were present in exosomes, however, they were not able to establish productive infection in uninfected cells. Exosomes isolated from RSV-infected cells were able to activate innate immune response by inducing cytokine and chemokine release from human monocytes and airway epithelial cells. These data suggest that exosomes may play an important role in pathogenesis or protection against disease, therefore understating their role in RSV infection may open new avenues for target identification and development of novel therapeutics.
Analysis of Combined Transcriptomes Identifies Gene Modules that Differentially Respond to Pathogenic Stimulation of Vascular Smooth Muscle and Endothelial Cells
Authors: Pan Xiaokang , Wang Bowen , Yuan Tiezheng , Zhang Mengxue , Kent K. Craig , Guo Lian-Wang
Published: January 2018

Smooth muscle cells (SMCs) and endothelial cells (ECs) are vital cell types composing the vascular medial wall and the atheroprotective inner lining, respectively. Current treatments for cardiovascular disease inhibit SMC hyperplasia but compromise EC integrity, predisposing patients to thrombosis. Therapeutics targeting SMCs without collateral damage to ECs are highly desirable. However, differential (SMC versus EC) disease-associated regulations remain poorly defined. We conducted RNA-seq experiments to investigate SMC-versus-EC differential transcriptomic dynamics, following treatment of human primary SMCs and ECs with TNFα or IL-1β, both established inducers of SMC hyperplasia and EC dysfunction. As revealed by combined SMC/EC transcriptomes, after TNFα or IL-1β induction, 174 and 213 genes respectively showed greater up-regulation in SMCs than in ECs (SMC-enriched), while 117 and 138 genes showed greater up-regulation in ECs over SMCs (EC-enriched). Analysis of gene interaction networks identified central genes shared in the two SMC-enriched gene sets, and a distinct group of central genes common in the two EC-enriched gene sets. Significantly, four gene modules (subnetworks) were identified from these central genes, including SMC-enriched JUN and FYN modules and EC-enriched SMAD3 and XPO1 modules. These modules may inform potential intervention targets for selective blockage of SMC hyperplasia without endothelial damage.
Genome-wide association study in Asia-adapted tropical maize reveals novel and explored genomic regions for sorghum downy mildew resistance
Authors: Rashid Zerka , Singh Pradeep Kumar , Vemuri Hindu , Zaidi Pervez Haider , Prasanna Boddupalli Maruthi , Nair Sudha Krishnan
Published: January 2018

Globally, downy mildews are among the important foliar diseases of maize that cause significant yield losses. We conducted a genome-wide association study for sorghum downy mildew (SDM;Peronosclerospora sorghi) resistance in a panel of 368 inbred lines adapted to the Asian tropics. High density SNPs from Genotyping-by-sequencing were used in GWAS after controlling for population structure and kinship in the panel using a single locus mixed model. The study identified a set of 26 SNPs that were significantly associated with SDM resistance, with Bonferroni corrected P values ≤ 0.05. Among all the identified SNPs, the minor alleles were found to be favorable to SDM resistance in the mapping panel. Trend regression analysis with 16 independent genetic variants including 12 SNPs and four haplotype blocks identified SNP S2_6154311 on chromosome 2 with P value 2.61E-24 and contributing 26.7% of the phenotypic variation. Six of the SNPs/haplotypes were within the same chromosomal bins as the QTLs for SDM resistance mapped in previous studies. Apart from this, eight novel genomic regions for SDM resistance were identified in this study; they need further validation before being applied in the breeding pipeline. Ten SNPs identified in this study were co-located in reported mildew resistance genes.
Responses of Intrinsic Water-use Efficiency and Tree Growth to Climate Change in Semi-Arid Areas of North China
Authors: Weiwei L. U. , Xinxiao Y. U. , Guodong J. I. A. , Hanzhi L. I. , Ziqiang L. I. U.
Published: January 2018

Tree-level intrinsic water-use efficiency (iWUE) is derived from the tree-ring 13C isotope composition (δ13C) and is an important indicator of the adaptability for trees to climate change. However, there is still uncertainty regarding the relationship between long-term forest ecosystem carbon sequestration capacity and iWUE. To determine whether elevated atmospheric CO2 concentration (Ca) increase iWUE and tree growth (basal area increment, BAI), dendrochronological methods and stable isotope analyses were used to examine annual changes in the tree-ring width and carbon isotope composition (δ13C) of Platycladus orientalis in northern China. The iWUE derived from δ13C has increased significantly (p < 0.01). Long-term iWUE trend was largely and positively driven by the elevated atmospheric CO2 concentration and temperature. We observed a general increase in averaged BAI, which had significant positive correlation with iWUE (R2 = 0.3186, p < 0.01). Increases in iWUE indeed translated into enhanced P. orientalis growth in semi-arid areas of northern China. Elevated atmospheric CO2 concentration significantly (p < 0.01) stimulated P. orientalis biomass accumulation when Ca was less than approximately 320 ppm in the early phase; however, this effect was not pronounced when Ca exceeded 320 ppm.
Comparative analysis of low complexity regions in Plasmodia
Authors: Chaudhry S. R. , Lwin N. , Phelan D. , Escalante A. A. , Battistuzzi F. U.
Published: January 2018

Low complexity regions (LCRs) are a common feature shared by many genomes, but their evolutionary and functional significance remains mostly unknown. At the core of the uncertainty is a poor understanding of the mechanisms that regulate their retention in genomes, whether driven by natural selection or neutral evolution. Applying a comparative approach of LCRs to multiple strains and species is a powerful approach to identify patterns of conservation in these regions. Using this method, we investigate the evolutionary history of LCRs in the genus Plasmodium based on orthologous protein coding genes shared by 11 species and strains from primate and rodent-infecting pathogens. We find multiple lines of evidence in support of natural selection as a major evolutionary force shaping the composition and conservation of LCRs through time and signatures that their evolutionary paths are species specific. Our findings add a comparative analysis perspective to the debate on the evolution of LCRs and harness the power of sequence comparisons to identify potential functionally important LCR candidates.
8u, a pro-apoptosis/cell cycle arrest compound, suppresses invasion and metastasis through HSP90α downregulating and PI3K/Akt inactivation in hepatocellular carcinoma cells
Authors: Wang Ning , Chen Shaopeng , Zhang Bin , Li Shangfu , Jin Feng , Gao Dan , Liu Hongxia , Jiang Yuyang
Published: January 2018

8u, an acridine derivative, has been proved effective anti-hepatocarcinoma effect, while the underlying mechanism remains unclear. Here, metabolomics and proteomics approaches were applied to study its anti-cancer mechanism and explore its effect on HepG2 cells’ invasion and metastasis abilities. The results showed that 8u significantly suppressed HepG2 cells migration and enhanced cell-to-cell junctions. The inhibition effect of 8u on invasion and metastasis disappeared after HSP90α gene silencing, and was reversed after HSP90α overexpression. The biological experimental results indicated that 8u also blocked PI3K/Akt pathway, thereby reducing fatty acid synthase (FASN) protein expression and disordering intracellular lipid metabolism to inhibit cell invasion and metastasis. In addition, HSP90α protein and PI3K/Akt pathway could co-adjust to each other. These findings demonstrated that 8u could efficiently suppress the invasion and metastasis of HepG2 cells by decreasing the expression of HSP90α protein and inhibiting the PI3K/Akt signaling pathway, which could be used as a potential candidate for the treatment of HCC.
Exploring the consequences of social defeat stress and intermittent ethanol drinking on dopamine dynamics in the rat nucleus accumbens
Authors: Deal Alex L. , Konstantopoulos Joanne K. , Weiner Jeff L. , Budygin Evgeny A.
Published: January 2018

The current study aimed to explore how presynaptic dopamine (DA) function is altered following brief stress episodes and chronic ethanol self-administration and whether these neuroadaptations modify the acute effects of ethanol on DA dynamics. We used fast-scan cyclic voltammetry to evaluate changes in DA release and uptake parameters in rat nucleus accumbens brain slices by analyzing DA transients evoked through single pulse electrical stimulation. Adult male rats were divided into four groups: ethanol-naïve or ethanol drinking (six week intermittent two-bottle choice) and stressed (mild social defeat) or nonstressed. Results revealed that the mild stress significantly increased DA release and uptake in ethanol-naïve subjects, compared to nonstressed controls. Chronic ethanol self-administration increased the DA uptake rate and occluded the effects of stress on DA release dynamics. Bath-applied ethanol decreased stimulated DA efflux in a concentration-dependent manner in all groups; however, the magnitude of this effect was blunted by either stress or chronic ethanol, or by a combination of both procedures. Together, these findings suggest that stress and ethanol drinking may promote similar adaptive changes in accumbal presynaptic DA release measures and that these changes may contribute to the escalation in ethanol intake that occurs during the development of alcohol use disorder.