bluuebunny/crossref_metadata_2025 huggingface dataset:
Phytolith assemblages from palm leaves and palm-leaf manuscripts: what is the difference and what it could mean?
Authors: Poliakova Anastasia , Ciotti Giovanni , Helman-Wazny Agnieszka , Fromm Jörg
Published: January 2025

We studied freshly collected, dried and herbarized leaf fragments of two palms, namely Borassus flabellifer L. and Corypha umbraculifera L., most commonly used for palm-leaf manuscript (PLM) production in South (S) and Southeast Asia (SE) in order to reveal differences in their phytolith assemblages. For each of the two palms, 25 leaf samples were taken from the two Indian states of Kerala and Tamil Nadu. Dried leaf material was obtained from the fresh one by drying the leaves in air. Herbarium samples were obtained from two independent herbaria, specimen origin comprises S and SE Asia with the main focus on South India and Sri Lanka. Additionally, 25 manuscripts made of Borassus flabellifer leaves and 25 manuscripts made of Corypha umbraculifera leaves were investigated for phytoliths. All manuscripts are preliminary dated back to between the 16th and the beginning of the 20th century CE; most of them assumedly were produced in S India (Tamil Nadu and Kerala), Sri Lanka, Burma or Indonesia. Phytolith assemblages significantly differed between fresh, dry and herbarized palm leaves in comparison to PLM material, both qualitatively and quantitatively (mean r2 = - 0.61 ± 9.3 for Borassus samples and r2 = - 0.75 ± 5.3 for Corypha samples, at p < 0.001). Fifty-three phytolith types described for PLM material were not observed in any of the fresh, dry or herbarized palm-leaf samples. Geographical analysis of PLM-specific phytoliths suggests that the combination of those phytoliths could be region-related. In this paper, we prove that the methods of palaeoecological reconstructions based on detailed microscopy of the PLMs surface and phytolith analysis applied in combination with methods of mathematical and computer data analysis can contribute to answer the questions posed by material codicology by revealing lost manuscript production recipes and by studying manuscript provenance in terms of the geographical origin of the artefacts. Our approach can potentially open a new perspective for palaeoecological studies expanding their traditional scope and making them applicable to a new research field.
Utilizing physiologies, transcriptomics, and metabolomics to unravel key genes and metabolites of Salvia miltiorrhiza Bge. seedlings in response to drought stress
Authors: Zhang Yu , Zhang Hong , Zhang Yuru , Wang Di , Meng Xue , Chen Juan
Published: January 2025

Drought stress inhibits Salvia miltiorrhiza Bunge (S. miltiorrhiza) seedling growth and yield. Here, we studied the effects of drought stress on the different parts of S. miltiorrhiza seedlings through physiological, transcriptomic, and metabolomics analyses, and identified key genes and metabolites related to drought tolerance. Physiological analysis showed that drought stress increased the accumulation of hydrogen peroxide (H2O2), enhanced the activity of peroxidase (POD), decreased the activity of catalase (CAT) and the contents of chlorophyll b and total chlorophyll, reduced the degree of photosynthesis, enhanced oxidative damage in S. miltiorrhiza seedlings, and inhibited the growth of S. miltiorrhiza plants. Transcriptome analyses revealed 383 genes encoding transcription factors and 80 genes encoding plant hormones as hypothetical regulators of drought resistance in S. miltiorrhiza plants. Moreover, differentially expressed genes (DEGs) and differentially expressed metabolites (DEMs) are involved in a variety of biological processes, such as proline and glycine betaine metabolism, and biosynthesis of tanshinones and phenolic acids. Additionally, it has barely been reported that the AHL gene family may be involved in regulating the neocryptotanshinone biosynthesis. In conclusion, our results suggest that drought stress inhibits S. miltiorrhiza seedling growth by enhancing membrane lipid peroxidation, attenuating the antioxidant system, photosynthesis, and regulating proline and glycine betaine metabolism, transcription factors and plant hormones, and tanshinones and phenolic acid metabolism pathways. This study provides new insights into the complex mechanisms by which S. miltiorrhiza responds to drought stress.
Response of the germinable soil seed bank of temperate Leymus chinensis meadows to mowing regimes
Authors: Zhang Zhitao , Yu Tianqi , Xin Xiaoping , Liu Hongmei , Lv Shijie , Wei Zhijun , Han Guodong , Yan Ruirui
Published: January 2025

Mowing is a primary practice in temperate L. chinensis meadows, which are severely degraded due to frequent mowing, overgrazing, and other factors, necessitating restoration and sustainable management. The natural recovery of these grasslands hinges on their germinable soil seed banks, which form the basis for future productivity. Thus, germinable soil seed banks are critical for restoring overexploited meadows. In this study, we conducted germination experiments on 135 soil samples from various depths to comprehensively analyze the germinable seed bank under different mowing regimes. The main results were as follows: (1) the germinable soil seed bank density decreased significantly with a mowing event per year (C1), and the number of perennial grass seeds and upper grass seeds also decreased under the mowing event per year; (2) the size of the germinable soil seed bank increased under the other mowing regimes (control area without mowing or grazing, CK; mowing event every 2 years, C2; mowing event every 3 years, C3; and mowing event every six years, C6) relative to that under once-a-year mowing. With increasing soil depth, the number of germinable soil seeds decreased significantly. Most of the seeds in the germinable soil seed banks were distributed in the 0–2 cm soil layer, accounting for approximately 80% of the total, and at depths of 5–10 cm, the number of seeds of upper grasses was greater than that of perennial grasses. (3). During the mowing event each year, the seed bank of germinable soil seeds significantly decreased. Mowing every 2 years provides a one-year interval for natural vegetation growth, allowing for greater retention of seeds in the germinable soil seed bank. Mowing every 6 years significantly reduces the disturbance frequency, providing ample time for plant reproduction and resulting in the accumulation of germinable seeds in the soil.
“I think we're on a cusp of some change:” coping and support for mental wellness among Black American women
Authors: McCall Terika , Foster Meagan , Tomlin Holly , Adepoju Bolatito , Bolton-Johnson Mckenzie , Bellamy Chyrell D.
Published: January 2025

Introduction

Public discussions in the media (e.g., social media and reality shows) about Black women's mental health have become more common. Notably, celebrities have become more vocal about their own mental health challenges and sought to normalize seeking care. This study aimed to gain a better understanding of Black women's: (1) past and current causes of stress, anxiety, and depression, and coping skills used; (2) their attitudes and perceptions toward mental health and receiving mental health treatment; and (3) times in their life they felt anxious or depressed, and what type of support or resources would have been helpful to have access to.

Methods

Focus groups were conducted with 20 women (mean age 36.6 years, SD 17.8 years), with 5 participants per group. Descriptive qualitative content analysis of the focus group transcripts was conducted.

Results

Results consistently showed that intersectional identities of being both Black and a woman resulted in feelings of both hypervisibility and invisibility, representation matters when it comes to mental health providers, an increased openness to therapy across age groups, and a willingness to try digital health tools (e.g., smartphone app) for mental health needs. There is still work to be done to normalize mental health treatment among Black women.

Discussion

Subgroups within the community (e.g., young adults) have less stigma around mental health and are acting as catalysts for change. Intentional inclusion of Black women in mental health research and evolving treatment paradigms is important to eliminate inequities in access to culturally relevant mental health care.
The impact of collaborative atmosphere on innovative work behavior of college teachers, North China
Authors: Liu Qian , Sun Yu
Published: January 2025

Innovation and progress serve as the driving forces behind national development. Universities, with their comprehensive academic systems and robust research capabilities, undoubtedly play a crucial role in fostering student innovation and advancing faculty research innovation. This study aims to explore the relationship between the collaborative climate and innovative work behavior of university educators, as well as the mediating effect of knowledge sharing, in order to provide an important theoretical basis for universities to better promote innovative work behavior. This study adopts questionnaire survey method and semi-structured interview method. In the questionnaire survey stage, this study uses 473 in-service educators in colleges and universities as the research objects; in the interview stage, this study uses 8 in-service educators as the research objects. The results of the questionnaire study prove that educators’ cooperation atmosphere has a significant positive impact on innovative work behavior, educators’ collaborative climate has a significant positive impact on knowledge sharing, knowledge sharing has a significant positive impact on innovative work behavior, and knowledge sharing has a significant positive impact on teachers’ collaborative climate. There is a significant mediating effect on employees’ innovative work behavior. The interview results found that a positive collaborative climate within universities can influence teachers’ innovative work behavior through three channels: colleague support, management resource provision, and academic freedom encouragement. Therefore, a positive collaborative climate not only encourages communication and cooperation among faculty members but also inspires them to adopt and develop new methods and technologies in their research and teaching practices. Universities should place greater emphasis on enhancing their internal collaborative atmosphere.
Relationship between physical activity and college students’ life satisfaction: the chain mediating effect of psychological resilience and negative emotions
Authors: Huang Chenxin , Wang Jinfu , Chang Zixuan , Tang Jianjuan
Published: January 2025

Objective

As the academic pressure, employment competition and mental health problems faced by college students are becoming more and more prominent, paying attention to and improving the quality of life and well-being of college students has become an important issue of widespread concern in all walks of life. This study focuses on the correlation between physical activity and college students’ life satisfaction.

Methods

A cross-sectional survey method was applied to 326 college students, using the Physical Activity Rating Scale, the Psychological Resilience Scale, the Depression-Anxiety-Stress Scale, and the Life Satisfaction Scale. For data analysis, demographic analysis of variance, correlation analysis, and chain mediating effect test were conducted sequentially.

Results

There were significant differences in psychological resilience, negative emotions, and life satisfaction by gender, and psychological resilience by grade level; there were significant correlations between physical activity and psychological resilience, negative emotions, and life satisfaction among college students (r = 0.541, p < 0.001; r = −0.379, p < 0.001; r = 0.435, p < 0.001); and psychological resilience, negative emotions had significant mediating and chain mediating effects between physical activity and life satisfaction, where the mediating effect of psychological resilience was significantly stronger than the mediating effect of negative emotions and the chain mediating effect of both.

Conclusion

There was a correlation between physical activity and life satisfaction among college students, and this relationship was partially mediated by psychological resilience and negative emotions.
The integration and innovative practice of intelligent AI and local opera in college teaching
Authors: Li Chunying
Published: January 2025

This paper explores the impacts of integrating AI into the teaching of Chinese Opera using a mixed-methods approach, examining performance, engagement, and psychological factors in students. A quasi-experimental design involving 199 participants over a one-year period was conducted, involving teaching with and without AI enhancement. Quantitative data, derived from standardized tests and analytics provided by AI platforms, were supported by qualitative data from interviews and observational studies. These results suggest that there were significant increases in the AI-enhanced cohort in opera performance competencies (Δ = 13.6, p < 0.001); retention of cultural knowledge (Δ = 15.5, p < 0.001), and overall engagement levels (r = 0.73, p < 0.001). Time series analysis revealed nonlinear learning trajectories, with participants showing greatest gains during the intervention’s midpoint. The psychological data showed a strengthening relationship between self-efficacy and in-performance outcomes, demonstrating an increase from r_initial = 0.38 to r_final = 0.67, p < 0.001. This study indicates both the potential of AI in the preservation and development of traditional artistic work and highlights some challenges in initial implementation. The findings facilitate the ongoing discussion of integrating technologies into arts education and provide valuable insights to support curriculum development, in addition to conserving cultural heritage in the modern-day digital world.
Budget impact analysis of insulin glargine vs. isophane protamine insulin treatment for patients with type-2 diabetes and severe hypoglycemia in Thailand
Authors: Permsuwan Unchalee , Deerochanawong Chaicharn
Published: January 2025

Objective

This study aimed to assess the financial impact of different adoption rates of insulin glargine (IGlar) treatment compared to isophane protamine (neutral protamine hagedorn [NPH]) insulin treatment for patients with type-2 diabetes (T2D) and severe hypoglycemia in Thailand from the payer’s perspective.

Methods

The budget impact analysis (BIA) model over a period of 5 years was used to estimate the net budget impact (NBI) of IGlar treatment by comparing the total budget expenditures under two scenarios: scenario 1 involved only NPH insulin and scenario 2 included the introduction of IGlar. The total budget included either the cost of insulin or a combination of the costs of insulin and the expense related to severe hypoglycemia. Scenario 2 started at 20% uptake of IGlar and a yearly increase of 20%. NBI was calculated as the difference between the total budgets of scenarios 1 and 2. NBI and one-way sensitivity analyses were conducted for evaluation.

Results

Considering only the cost of insulin, the use of IGlar for patients with T2D and severe hypoglycemia resulted in a yearly average NBI of 174.9 million Thai baht (THB) (5.1 million USD). However, when the cost related to severe hypoglycemia was included, the total budget incurred from scenario 2 was less than that of scenario 1, leading to a negative NBI or cost savings.

Conclusion

The NBI of IGlar adoption would be substantial when considering only the cost of insulin; however, the significant benefit of IGlar in terms of a lower rate of severe hypoglycemia compared with NPH insulin would clearly offset the additional cost of IGlar.
Understanding the cost-utility of implementing HIV self-testing with digital-based supports
Authors: Empringham Brianna , Karellis Angela , Fernandez-Suarez Marta , Carmona Sergio , Pai Nitika Pant , Zwerling Alice
Published: January 2025

Introduction

HIV self-testing (HIVST) is an innovative strategy that has been shown to increase uptake of HIV testing compared to conventional facility-based testing. HIVST implementation with digital-based supports may help facilitate testing accessibility and linkage to care after a reactive self-test. Economic evidence around community-based implementation of HIVST is growing; however, economic evidence around digital-based HIVST approaches remains limited.

Methods

We used previously published cost and efficacy data from HIVST interventions, with the specific intervention model differing between scenarios. Digital-based interventions included text messaging campaigns and online websites that promoted uptake and linkage to HIVST care. Community-based interventions included door-to-door distribution, peer-incentivized distribution, and mobile testing units. Using data obtained from the literature, we parameterized a combined Markov and decision analytic model to evaluate the cost-utility of digital-based HIVST implementation across Malawi, South Africa, and Brazil compared to both community-based HIVST and facility-based testing.

Results

We found that HIVST was cost-effective compared to facility-based testing in all settings investigated. Our scenarios predicted that digital-based HIVST was associated with an incremental cost in the range of $769–$17,839/DALY (disability-adjusted life year) averted compared to facility-based testing across Malawi, South Africa, and Brazil. Digital-based HIVST cost savings had an incremental cost of $7,300/DALY averted compared to community-based HIVST. The main drivers of cost-utility included HIV test and treatment costs, HIV test-positivity, rates of linkage to care, and antiretroviral therapy (ART) initiation rates. Digital-based supports were associated with an increased cost compared to facility-based testing, but they also had increased utility, which led to favorable cost-utility estimates.

Discussion

HIVST with digital supports has the potential to be a highly cost-effective approach, with the potential to make HIV testing more available and accessible, thereby increasing overall uptake and coverage of HIV testing. Digital supports can also support linkage to care, which we have identified as a major driver of cost-utility. Strategies to improve cost-utility include reducing testing costs, targeting key populations with increased rates of HIV test-positivity, and ensuring strong support for linkage to care.
An analysis of the current status of geriatric oral disease treatment—a dental institutions-based perspective
Authors: Li Caili , Huang Dongmei , Pan Qini , Tao Pinyue , Pan Xiao , Pan Yanfei , He Ruofei , Wang Tongting , Huang Huiqiao
Published: January 2025

Background

This study aimed to investigate and analyze the current status of oral disease treatment among the older adult in Guangxi Zhuang Autonomous Region, while also assessing the continuing medical education (CME) needs of dental institution personnel regarding oral diseases in this population.

Methods

Convenience sampling was used to investigate the oral disease treatment among older adults and to assess CME needs of dental institution personnel regarding oral diseases in this population across various oral medical and health institutions in Guangxi.

Results

A total of 754 valid questionnaires were collected, of which 70.3% were from non-public oral health institutions. Out-of-pocket costs for older adults were as high as 91.3%. The per capita cost of older adult patients was beyond 500 yuan in 51.6% of the oral health institutions. In terms of CME training, 32.8% of dental institution medical personnel have participated in CME courses specifically on oral diseases in the older adult. Meanwhile, 69.9% of institutions have expressed a need for CME training on oral diseases in the older adult.

Conclusion

Non-public oral health institutions account for a significant proportion, and the older adult primarily pay out-of-pocket for oral disease treatment in these facilities. The high cost of treatment may pose a significant barrier to the older adult seeking oral healthcare. Increasing CME programs targeted at geriatric oral diseases can help enhance the treatment capabilities of dental healthcare workers and improve oral health outcomes for the older adult population.
Current status of public health awareness and roles within medical institutions in eastern China
Authors: Wu Yuchen , Zhang Chuanfeng , She Quan , Zheng Wei , Lu Kai , Wang Furu
Published: January 2025

Objective

To examine the current landscape of public health functions and the level of cognition among medical staff across various medical institutions in Jiangsu Province, ultimately aiming to provide a theoretical framework for constructing a hospital public health work model under the integrated medical and defense system.

Methods

A mixed-methods approach involving questionnaires and semi-structured interviews was employed to assess the establishment and operational status of public health departments within medical institutions, staffing scenarios, comprehension of public health tasks, and the degree of emphasis placed on them.

Results

Our findings reveal that 70.6% of medical institutions possess standalone public health departments, with a median of 5 full-time public health personnel, predominantly from the nursing profession. Significant disparities were observed in the demographics, educational backgrounds, majors, job classifications, and titles of public health personnel across different medical institution levels. Additionally, 68.4% of institutions have formed public health management committees, and 77.2% shoulder ≥10 public health responsibilities. Notably, 99.2% of medical staff endorse the importance of public health work within medical institutions, and 98.3% advocate for the support of disease control agencies. Interviews further unveiled a predominantly positive stance among medical institution leaders toward the integration of public health and medical prevention.

Conclusion

Medical institutions of Jiangsu Province necessitate enhanced public health staffing, improved organizational structure and responsibility divisions for public health work, and a boost in knowledge levels to effectively carry out public health responsibilities. Furthermore, policy support and multi-stakeholder engagement are crucial to mobilize hospitals to take proactive initiatives.
Emotion recognition deficits in children and adolescents with autism spectrum disorder: a comprehensive meta-analysis of accuracy and response time
Authors: Masoomi Maryam , Saeidi Mahdieh , Cedeno Rommy , Shahrivar Zahra , Tehrani-Doost Mehdi , Ramirez Zerimar , Gandi Divya Aishwarya , Gunturu Sasidhar
Published: January 2025

Background

Autism spectrum disorder is a neurodevelopmental condition characterized by persistent challenges in social communication and restricted, repetitive behaviors. Emotion recognition deficits are a core feature of ASD, impairing social functioning and quality of life. This meta-analysis evaluates emotion recognition accuracy and response time in individuals with autism spectrum disorder compared to neurotypical individuals and those with other neurodevelopmental disorders.

Methods

This systematic review with a meta-analysis was conducted following PRISMA guidelines. A comprehensive literature search across PubMed, Scopus, Cochrane Library, and Web of Science identified 13 studies published between 2006 and 2024. Data on emotion recognition accuracy and response times were synthesized using standardized mean differences in random-effects models. Heterogeneity was assessed using the I2 statistic, and sensitivity analyses were performed to ensure robustness.

Results

Individuals with ASD exhibited significantly lower overall emotion recognition accuracy compared to TD individuals (SMD = −1.29, 95% CI: −2.20 to −0.39, p < 0.01) and NDDs (SMD = −0.89, 95% CI: −1.23 to −0.55, p = 0.02). Response times were significantly prolonged in ASD compared to TD individuals (SMD = 0.50, 95% CI: 0.36–0.63, p < 0.01) but not when compared to NDDs. Emotion-specific analyses did not consistently reveal significant differences across emotions (fear, anger, happiness, sadness, disgust, surprise), with substantial heterogeneity observed across studies (I2 > 50%).

Conclusions

This systematic review with a meta-analysis highlights significant impairments in emotion recognition accuracy and processing speed among individuals with autism spectrum disorder, particularly compared to neurotypical individuals. These findings underscore the importance of developing targeted interventions to address these deficits, which are foundational to improving social cognition and quality of life in autism spectrum disorder. Future research should prioritize standardized methodologies and explore cultural and contextual factors influencing emotion recognition abilities.

Systematic Review Registration

https://www.crd.york.ac.uk/PROSPERO/display_record.php?RecordID=627339, PROSPERO (CRD42024627339).
Physical Literacy—Being Active to Live Your Best Life
Authors: Goss Hannah , Belton Sarahjane , O’Brien Wesley , Duncan Michael J. , Foweather Lawrence
Published: January 2025

Being active is good for both our minds and bodies, but many people worldwide are not moving enough. There are lots of reasons for this, and there are lots of ways we can help people to move more. Just like literacy is about our ability to read and write, physical literacy is about our ability to be active, as well as understanding what we enjoy and value about being active. Our thoughts, feelings, and all the physical activities that we have done before impact how we feel about moving. Playing in a park with friends and trying hard to catch a ball are examples of things that can change how we think about being active, now and in the future. Physical literacy is different for everyone, and it changes all the time. This article will explain what physical literacy is and why it is a very important part of growing up.
Effects of grazing on alpine grassland soil available nutrients across the Tibetan Plateau
Authors: Zhou Tongyong , Yang Hui , Du Yangong
Published: January 2025

Introduction

Alpine grasslands on the Tibetan Plateau are increasingly affected by grazing, resulting in complex changes in soil nutrient availability.

Methods

This meta-analysis evaluates the effects of different grazing intensities on soil available nitrogen (AN), phosphorus (AP), and potassium (AK), and identifies key environmental drivers.

Results

Results show that grazing significantly increased AN by 11.9% and reduced AK by 14.5%, while its impact on AP was negligible. Light grazing (LG) and moderate grazing (MG) both significantly enhanced AN, with increases of 6.88% and 17.90%, respectively, whereas heavy grazing (HG) reduced AN by 1.52%. LG and MG also positively affected AP, whereas HG caused a substantial decline of 11.04%. In terms of AK, LG led to a slight increase (2.05%), but MG and HG both caused significant reductions of 11.01% and 10.35%, respectively. Temperature emerged as a critical factor, reducing AN and increasing AK, highlighting its importance under climate change scenarios.

Discussion

These findings emphasize that grazing intensity plays a pivotal role in regulating soil nutrient levels, with LG showing the most consistent positive effects on nutrient availability. Thus, managing grazing intensity, particularly favoring light grazing, is crucial to maintaining soil fertility and supporting the long-term productivity of alpine grasslands on the Tibetan Plateau.
Alternatywne metody głosowania w opiniach Polaków głosujących poza granicami państwa
Authors: Musiał-Karg Magdalena
Published: January 2025

The debate on alternative forms of participation in elections and referenda is conducted not only in the context of the time and situation in which they are used, but also, and most importantly, in the context of different groups of people who are eligible to vote, in particular the disabled and senior citizens. It is worth noting that Poles living abroad do not vote in all national elections (e.g. local government elections). However, in recent years the discussion on alternative forms of voting has begun to include this group of Polish citizens, especially in the context of postal and online voting (e-voting). The aim of this paper is to present and analyse the results of the research carried out on the opinions of Poles voting abroad in the 2023 parliamentary elections on their preferred method of voting. One of the aims of the research was to answer the question of which groups of voters (by gender, age, education) are most interested in certain forms of voting, and whether party preferences differentiate declarations of preferred voting methods.
Characterisation of the Pathogenicity of Beauveria sp. and Metarhizium sp. Fungi Against the Fall Armyworm, Spodoptera frugiperda (Lepidoptera: Noctuidae)
Authors: Apirajkamol Nonthakorn (Beatrice) , Mainali Bishwo , Taylor Phillip Warren , Walsh Thomas Kieran , Tay Wee Tek
Published: January 2025

Previously, we assessed the pathogenicity of eleven endemic entomopathogenic fungi (EPF), including six Beauveria isolates, four Metarhizium isolates, and one M. pingshaense, against the agricultural pest Spodoptera frugiperda (fall armyworm, FAW). We found that four Beauveria and one Metarhizium isolates were effective, with Beauveria isolates B-0571 and B-1311 exhibiting high mortality within 24 h post-spore application. This study aimed to identify and characterise the entomopathogenesis mechanisms of these isolates as potential FAW biocontrol agents. All Beauveria isolates were determined as B. bassiana, the Metarhizium isolates as two M. robertsii, one M. majus, and an unknown Metarhizium species. Despite the high mortality from B-0571 and B-1311 isolates, scanning electron microscopy showed no fungal spore germination on dead larvae 24 h after spore application. Four insecticide compound gene clusters, i.e., bassianolide, beauvericin, beauveriolide, and oosporein, were identified and characterised in all B. bassiana isolates. These compounds are hypothesised to contribute to the high early mortality rates in FAWs. Identifying and characterising gene clusters encoding these insecticide compounds in B-0571 and B-1311 will contribute to a better understanding of the entomopathogenicity of these isolates that will be vital to developing these EPF isolates as sustainable alternative FAW biocontrol agents.
Effect of Green Roofs on the Thermal Environment of Prototype Broiler Houses
Authors: Souza Maria Angela de , Sousa Fernanda Campos de , Silva Alex Lopes da , Soares Thauane Cordeiro , Oliveira Charles Paranhos , Vigoderis Ricardo Brauer , Baêta Fernando da Costa , Tinôco Ilda de Fátima Ferreira
Published: January 2025

The management of thermal environments in animal production facilities presents significant challenges, requiring continuous adjustments to meet animals’ physiological needs. This study evaluated the effects of green roofs on the thermal environment and comfort indices in small-scale poultry house prototypes, comparing facilities with and without green roof installations. The research tested various roof types (ceramic, fiber cement, and metal) combined with emerald grass (Zoysia japonica) green roof systems. Parameters measured included air temperature, relative humidity, internal roof surface temperature, Temperature and Humidity Index (THI), Black Globe Humidity Index (BGHI), Human Comfort Index (HCI), and Thermal Radiation Load (TRL) under both open and closed conditions. Results showed that green roofs reduced indoor air temperature by up to 2.4 °C in open prototypes and 10.6 °C in closed prototypes during peak heat periods. In combinations using green roofs with fiber cement tiles, internal roof surface temperature decreased by 24.0 °C in open prototypes and 27.0 °C in closed configurations. The implementation of green roofs resulted in THI reductions of 2.3 and 8.1 units in open and closed prototypes, respectively, BGHI decreases of 2.8 and 11.3 units, and TRL reductions of 21.0 W/m2 and 74.0 W/m2. HCI measurements confirmed improved thermal comfort conditions with green roof installations in both settings. This study concludes that green roofs effectively enhance the thermal environment by reducing bioclimatic indices during hot periods while maintaining stable conditions during cooler weather, thereby improving overall thermal comfort in animal facilities.
Application of Citizen Science to Sheep as a Model to Sensitize Young Citizens to Biodiversity, Animal Welfare and the Social Utility of Research
Authors: Moscatelli Sara , Paniccià Anna , Palmioli Elisa , Del Gobbo Laura , Mercati Francesca , Scocco Paola
Published: January 2025

Citizen science activities were performed using sheep as an animal model and involving 252 students aged between 9 and 11 years. The study focused on three pillars: hill/mountain landscape biodiversity, animal welfare and the social utility of research. Two types of tests—“attitude questionnaires” (AQs) and “maximum performance tests” (MPTs)—were administered. The AQ was administered before and at the end of the project to compare the children’s sensitivity to the treated topics and to assess their awareness of the project’s pillars. For each AQ item children expressed their agreement/disagreement by choosing from five response modes. The MPT aim was to evaluate if children had understood the explained topics by true/false options in respect to a sentence. The correlations between MPT scores and school evaluations for science subjects were analyzed. Among the fifteen AQ items specific to the pillars, four items related to biodiversity, three items related to animal welfare and two items related to the social utility of research, showing a significant difference (p < 0.05) for the second AQ’s responses. Two items showed sex-related differences. The analysis of the data from the MPT showed that the didactic methodology used was effective and improved the position of most children by bringing them into higher science evaluation groups.
Grazing-Induced Habitat Degradation: Challenges to Giant Panda Survival Resulting from Declining Bamboo and Soil Quality
Authors: Tian Huawei , Zeng Ying , Zhang Zejun , Lu Ming , Wei Wei
Published: January 2025

Grazing is the primary human-induced disturbance affecting giant panda (Ailuropoda melanoleuca) habitats and has a severe impact on the long-term sustainability of the giant panda population. To address the lack of quantitative studies on grazing’s impact on habitat quality, we selected China’s most heavily grazed giant panda nature reserve. Utilizing the Maxent model and stoichiometric analysis, we investigated habitat quality degradation caused by grazing and quantified changes in bamboo nutritional quality and soil physicochemical properties. The results indicate that grazing has significantly reduced the suitable habitat area for giant pandas from 101.87 km2 to 80.64 km2. Specifically, high-suitability habitats declined by 14.14%, moderate-suitability habitats declined by 22.70%, and low-suitability habitats declined by 22.88%. Grazing has forced pandas to move to higher altitudes (2650–3057 m) with taller (12–20 m) trees, denser (28–55 plants) shrubs, and sparser (30–69%) bamboo. Additionally, the soil water content has decreased, while soil bulk density, total N, available N, and pH have significantly increased. Reductions in crude protein and ether extract, along with increased crude fiber and ash, have lowered bamboo’s nutritional value and palatability. This study elucidates how grazing degrades giant panda habitat quality and provides a scientific basis for its conservation management.
Prepartum Vaccination Against Neonatal Calf Diarrhea and Its Effect on Mammary Health and Milk Yield of Dairy Cows: A Retrospective Study Addressing Non-Specific Effects of Vaccination
Authors: Kuhn Caroline , Zerbe Holm , Schuberth Hans-Joachim , Römer Anke , Kraatz-van Egmond Debby , Wesenauer Claudia , Resch Martina , Stoll Alexander , Zablotski Yury
Published: January 2025

Prepartum vaccinations against neonatal calf diarrhea pathogens are administered in late pregnancy to provide passive immunity to calves through protective colostral antibodies. Potential non-specific effects of the vaccine on maternal innate immune responses and disease susceptibility during the sensitive transition period have not been addressed so far. In this retrospective study, data from 73,378 dairy cows on 20 farms in Germany were analyzed, using linear mixed-effects regression, quantile regression, and decision-tree-algorithms, to investigate the effects of prepartum vaccination on mammary health and milk yield by comparing non-vaccinated and vaccinated transition periods. Herd management-related factors were found to be most influential for mammary health and milk yield. Vaccinated cows were not significantly more likely to develop mastitis and did not have significantly different somatic cell counts and milk yields compared to non-vaccinated cows. Healthy primiparous cows with and without vaccination had similar energy-corrected milk yields. The study concludes that prepartum vaccination against calf diarrhea has no significant effects on mammary health and milk yield. Further research is recommended to investigate potential non-specific vaccine effects on other organ systems, infectious diseases, and production metrics of the dairy cow.
Morphological and Molecular Identification of Obligatory Myiasis-Causing Species in Wild Cervids in Croatia
Authors: Gagović Ema , Jurković Žilić Daria , Pintur Krunoslav , Hodžić Adnan , Naletilić Šimun , Beck Relja
Published: January 2025

The infestation of ruminants by fly larvae of the Oestridae family can lead to nasopharyngeal or subcutaneous myiasis, which can harm animal welfare and productivity on sheep and cattle farms. The infestation of wild ruminants allows them to serve as reservoirs for subsequent transfer to domestic animals. The fly species most commonly responsible for myiasis have been well studied in many parts of the world, but less so in southeastern Europe. Here, we identified three botfly species among 197 subcutaneous larvae and 138 nasopharyngeal larvae from 36 wild roe deer (Capreolus capreolus) and 9 wild red deer (Cervus elaphus) at 30 locations across Croatia. Instars were stored in 75% ethanol and their species and developmental stage were identified based on morphological and morphometric keys. Subcutaneous larvae in both cervid species were identified as Hypoderma diana, while nasopharyngeal larvae were identified as Cephenemyia simulator in roe deer and Pharyngomyia picta in red deer. Partial sequencing of the gene-encoding mitochondrial cytochrome oxidase subunit I confirmed the taxonomic classification of C. stimulator and P. picta but was unable to confirm that of H. diana for lack of previously published sequences. This study appears to be the first to combine the morphological and molecular characterization of myiasis-causing botflies in wild cervids in southeastern Europe.
Ten Candidate Genes Were Identified to Be Associated with the Great Growth Differentiation in the Three-Way Cross Hybrid Abalone
Authors: Xiao Qizhen , Gong Shihai , Huang Zekun , Peng Wenzhu , Han Zhaofang , Gan Yang , Shen Yawei , You Weiwei , Ke Caihuan , Luo Xuan
Published: January 2025

Abalone is an economically important mollusk, whose slow growth has impeded the recovery of its wild populations and development of aquaculture. The three-way cross hybrid abalone ((Haliotis discus hannai♀ × H. fulgens♂)♀ × H. gigantea♂, DF × SS) demonstrated notable diversity in growth traits across the population with genetic differentiation, offering a model for exploring the molecular mechanisms of abalone growth. In this study, a total of 89 SNPs and 97 candidate genes were identified to be associated with growth-related traits of abalone using whole-genome resequencing and a genome-wide association study (GWAS) analysis. Then, ten overlap genes were found among these candidate genes by combining the results of GWAS and comparative transcriptomic analyses between the large individuals (L group) and small individuals (S group) of DF × SS. These overlap genes include up-regulated genes (fabG) and down-regulated genes (HMCN1, TLR3, ITIH3) between the L and the S groups, which are thought to function in growth in other organisms. The biological functions of these candidate genes in abalone still have to be confirmed, but they have improved our understanding of the molecular mechanisms behind abalone growth traits and provided molecular markers for abalone breeding programs.
Structure–Antioxidant Activity Relationship of Polysaccharides Isolated by Microwave/Ultrasonic-Assisted Extraction from Pleurotus ferulae
Authors: Zhou Hongjin , Fan Zhongxiong , Li Yuan , Liu Xuelian , Wang Bo , Xing Jianguo , He Jiang , Zheng Ruifang , Li Jinyao
Published: January 2025

To investigate the structure–antioxidant activity relationship, Pleurotus ferulae polysaccharides were extracted using ultrasonic (U-PFPS) and microwave/ultrasonic-assisted methods (MU-PFPS). Compared to U-PFPS with a molecular weight of 1.566 × 103 kDa, MU-PFPS exhibited a lower molecular weight of 89.26 kDa. In addition, unlike U-PFPS, which is primarily composed of glucose (Glu:Man:Gal = 91.1:3.5:5.4), MU-PFPS has a more balanced composition of Glu:Man:Gal in the ratio of 39.4:27.8:32.8 and contains more branched chains. Furthermore, antioxidant analysis revealed that high concentration (at concentrations above 600 μg/mL) MU-PFPS demonstrated stronger protective effects against oxidative damage in RAW264.7 cells than U-PFPS did. Collectively, these data suggest that lower molecular weight and higher branching degree of polysaccharides at appropriate concentrations may correlate with enhanced antioxidant enzyme activities. Our work provides a method for isolating polysaccharides with higher antioxidant activity and offers insights into the structure–activity relationship of polysaccharides, laying the foundation for future applications in polysaccharide modification and structural characterization.
Integrated Solution Combining Low-Frequency Forced Oscillation Technique and Continuous Equivital Sensor Monitoring for Assessment of Non-Invasive Ambulatory Respiratory Mechanics
Authors: Ben Othman Ghada , Ynineb Amani R. , Yumuk Erhan , Farbakhsh Hamed , Muresan Cristina , Birs Isabela Roxana , De Raeve Alexandra , Copot Cosmin , Ionescu Clara M. , Copot Dana
Published: January 2025

Early assessment of respiratory mechanics is crucial for early-stage diagnosing and managing lung diseases, leading to greater patient outcomes. Traditional methods like spirometry are limited in continuous monitoring and patient compliance as they require forced maneuvers with significant patient cooperation, which may not be available in fragile individuals. The Forced Oscillation Technique (FOT) is a non-invasive measurement method, only based on the tidal breathing at rest from the patient for a limited time period. The proposed solution integrates low-frequency FOT with continuous monitoring using Equivital (EQV) sensors to enhance respiratory mechanics information with heart rate variability. Data were collected over a two-hour period from six healthy volunteers, measuring respiratory impedance every 7 min and continuously recording physiological parameters. The best-fitting fractional-order models for impedance data were identified using genetic algorithms. This study also explores the correlation between impedance model parameters and EQV data, discussing the potential of AI tools for forecasting respiratory properties. Our findings indicate that combined monitoring techniques and AI analysis provides additional complementary information, subsequently aiding the improved evaluation of respiratory function and tissue mechanics. The proposed protocol allows for ambulatory assessment and can be easily performed in normal breathing conditions.
Influence of Proton Irradiation on Thin Films of AZO and ITO Transparent Conductive Oxides—Simulation of Space Environment
Authors: Ungeheuer Katarzyna , Rybak Janusz , Bocirnea Amelia E. , Pikulski Denis A. , Galca Aurelian C. , Marszalek Konstanty W.
Published: January 2025

Transparent conductive oxides are essential materials for many optoelectronic applications. For new devices for aerospace and space applications, it is crucial to know how they respond to the space environment. The most important issue in commonly used low-Earth orbits is proton radiation. This study examines the effects of high-energy proton irradiation (226.5 MeV) on thin films of aluminium-doped zinc oxide (AZO) and indium tin oxide (ITO). We use X-ray diffraction and electron microscopy observations to see the changes in the structure and microstructure of the films. The optical properties and homogeneity of the materials are determined by spectrophotometry and spectroscopic ellipsometry (SE). Analysis of the chemical states of the elements with X-ray photoelectron spectroscopy (XPS) gives insight into what proton irradiation changes at the surface of the oxides. All measurements show that ITO is less influenced than AZO. The proton energy and fluence used in this study simulate about a hundred years in low Earth orbit. This research demonstrates that both transparent conductive oxide thin films can function under simulated space conditions, with ITO showing superior resilience. The ITO film was more homogenous in terms of the total thickness measured with SE, had fewer defects and adsorbates present on the surface, as XPS analysis proved, and did not show a difference after irradiation regarding its optical properties, transmission, refractive index, or extinction coefficient.
Are Electromyography Data a Fingerprint for Patients with Cerebral Palsy (CP)?
Authors: Davoudi Mehrdad , Salami Firooz , Reisig Robert , Patikas Dimitrios A. , Beckmann Nicholas A. , Gather Katharina S. , Wolf Sebastian I.
Published: January 2025

This study aimed to first investigate changes in electromyography (EMG) patterns after multilevel surgical treatment in patients with cerebral palsy (CP) and then to assess the connection between the measure of EMG and motor control indices and surgery outcomes. We analyzed retrospective EMG and gait data from 167 patients with CP before and after surgery and from 117 typically developed individuals as a reference group. The patients underwent at least one soft tissue surgery on their shank and foot muscles. Using Repeated Measures ANOVA, we examined the norm-distance (ND) of the kinematics, kinetics, and EMG patterns, in addition to the Kerpape-Rennes EMG-based Gait Index (EDI), EMG Profile Score (EPS), and Walking Dynamic Motor Control Index (DMC) before and after surgery. Participants were divided into different response groups (poor, mild, and good gait quality) according to their pre- and post-treatment Gait Deviation Index (GDI), using the K-means-PSO clustering algorithm. The gait and EMG indices were compared between the responders using the nonparametric Mann–Whitney test. The ND for all kinematics and kinetics parameters significantly improved (p-value < 0.05) after the surgery. Regarding EMG, a significant reduction was only observed in the ND of the rectus femoris (p-value < 0.001) and soleus (p-value = 0.006). Among the indices, DMC was not altered post-operatively (p-value = 0.88). Although EDI and EPS were consistent across responders with a similar pre-treatment gait, a higher DMC was significantly associated with a greater improvement, particularly in patients with poor gait (p-value < 0.05). These findings indicate systematic changes in the EMG of patients with CP following surgery, which can also be demonstrated through indices. DMC is a measure that can potentially serve as a partial predictor of outcomes, particularly in patients with poor pre-operative gait. Future research should investigate the effects of different surgical strategies on the improvement of these patients.