bluuebunny/crossref_metadata_2025 huggingface dataset:
Craniofacial and Dental Manifestations of Melnick–Needles Syndrome: Literature Review and Orthodontic Management
Authors: Kustrzycka Dorota , Mikulewicz Marcin , Pelc Anna , Kosior Piotr , Dobrzyński Maciej
Published: November 2018

The aim of this article was to present a characteristic clinical image of Melnick–Needles syndrome using an example of an 11.5-year-old female patient treated at the Facial Congenital Disorders Outpatient Clinic as well as to present the actual literature review of the surgical treatment. The patient was diagnosed with several characteristics typical for Melnick–Needles syndrome: single-sided hearing loss, malocclusion, and facial dysmorphism, among others. Due to malocclusion and facial dysmorphism, the patient with Melnick–Needles syndrome requires orthodontic treatment with surgical intervention. Mandibular distraction with fixed appliance treatment is a recommended treatment protocol.
Pyroptosis of MCF7 Cells Induced by the Secreted Factors of hUCMSCs
Authors: Jiao Yang , Zhao Hongbo , Chen Guodong , Sang Xiongbo , Yang Luhan , Hou Zongliu , Si Wei , Zheng Bingrong
Published: November 2018

Human umbilical cord mesenchymal stem cells (hUCMSCs) are superior to other sources of mesenchymal stem/stromal cells (MSCs), and they are used as a novel tool for cell-based cancer therapy. However, the mechanism underlying hUCMSC-induced cancer cell death is not clear. In the present study, we aimed to evaluate the effect of secreted factors of hUCMSCs on the breast cancer cell line MCF7 by exposing them to the conditioned medium (CM) of hUCMSCs. We evaluated the morphological changes, cell viability, cell cycle, apoptosis, DNA fragmentation, and interleukin-1β (IL-1β) secretion of CM-exposed MCF7 cells. The results showed that the secreted factors of hUCMSCs could cause MCF7 cell death by inducing pyroptosis. We also sequenced the total RNA, and the differentially expressed genes (DEGs) were subjected to the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. A total of 2597 (1822 upregulated and 775 downregulated) genes were identified and 14 pathways were significantly enriched. The results showed that the expression of the pyroptosis-related genes NLRP1 and CASP4 and the inflammation-related pathways changed significantly in MCF7 cells exposed to the CM. To the best of our knowledge, this study is the first to report that the secreted factors of hUCMSCs can cause MCF7 cell pyroptosis. Furthermore, it is the first to examine the global gene expression in MCF7 cells exposed to CM. These results will provide valuable information for further studies on the mechanism of MCF7 cell pyroptosis induced by the secreted factors of hUCMSCs. It will also help understand the effect of hUCMSCs on cell-based breast cancer therapy.
Waterflood Management Using Hybrid Approach of Streamline‐Based Interwell Flux Information and Finite Volume Reservoir Simulation
Authors: Zhu Zhouyuan , Lei Zhengdong , Chen Zhangxin
Published: November 2018

In this work, we implement new software for improved waterflood management by combining classical finite volume reservoir simulation together with streamline tracing and corresponding interwell flux evaluations to optimize waterflood performance. We have introduced two basic modules here: a commercial reservoir simulator and our own streamline tracing and waterflood management program. Waterflood simulation is performed for a certain time span until simulation is paused, and the streamline tracing program is called to calculate interwell fluxes and adjust new well rates for better waterflood performance. The simulation continues afterwards until the next tracing and adjustment point is reached. The two modules work iteratively. The streamline tracing program is designed to trace streamlines on a compressible velocity field and a general corner point grid system with nonneighboring connections. The new injection rates are adjusted according to each well′s injection efficiency calculated from interwell multi‐phase fluxes. Streamline tracing is performed successfully not only on simple geometry corner point grid cases, but also on heavily faulted realistic reservoirs under waterflood. After readjusting injection rates multiple times during the simulation, we typically observe a reduction in field water cut of up to 5% and an increase in oil recovery in our test cases. Interwell flux information serves as effective diagnostic tools to identify injector‐producer pairs with large amount of water cycling. All simulations conducted here are rigorously finite volume based, which takes into account the full physics of nonadvective processes such as gravity and capillary effects. In conclusion, we have implemented a streamline‐based waterflood management program which works iteratively and cooperatively with a commercial reservoir simulator, without switching to streamline simulation. It provides an effective solution for improving oil recovery in brown fields by combining the rigorous mathematical nature of finite volume simulation and the power of streamline‐based flood management.
Electroacupuncture Treatment Alleviates the Remifentanil-Induced Hyperalgesia by Regulating the Activities of the Ventral Posterior Lateral Nucleus of the Thalamus Neurons in Rats
Authors: Zhao Hong-Yan , Liu Ling-Yu , Cai Jie , Cui Yan-Jun , Xing Guo-Gang
Published: November 2018

Mechanisms underlying remifentanil- (RF-) induced hyperalgesia, a phenomenon that is generally named as opioid-induced hyperalgesia (OIH), still remain elusive. The ventral posterior lateral nucleus (VPL) of the thalamus, a key relay station for the transmission of nociceptive information to the cerebral cortex, is activated by RF infusion. Electroacupuncture (EA) is an effective method for the treatment of pain. This study aimed to explore the role of VPL in the development of OIH and the effect of EA treatment on OIH in rats. RF was administered to rats via the tail vein for OIH induction. Paw withdrawal threshold (PWT) in response to mechanical stimuli and paw withdrawal latency (PWL) to thermal stimulation were tested in rats for the assessment of mechanical allodynia and thermal hyperalgesia, respectively. Spontaneous neuronal activity and local field potential (LFP) in VPL were recorded in freely moving rats using the in vivo multichannel recording technique. EA at 2 Hz frequency (pulse width 0.6 ms, 1–3 mA) was applied to the bilateral acupoints “Zusanli” (ST.36) and “Sanyinjiao” (SP.6) in rats. The results showed that both the PWT and PWL were significantly decreased after RF infusion to rats. Meanwhile, both the spontaneous neuronal firing rate and the theta band oscillation in VPL LFP were increased on day 3 post-RF infusion, indicating that the VPL may promote the development of RF-induced hyperalgesia by regulating the pain-related cortical activity. Moreover, 2 Hz-EA reversed the RF-induced decrease both in PWT and PWL of rats and also abrogated the RF-induced augmentation of the spontaneous neuronal activity and the power spectral density (PSD) of the theta band oscillation in VPL LFP. These results suggested that 2 Hz-EA attenuates the remifentanil-induced hyperalgesia via reducing the excitability of VPL neurons and the low-frequency (theta band) oscillation in VPL LFP.
Combined Lag Screw and Cerclage Wire Fixation for Calcaneal Tuberosity Avulsion Fractures
Authors: Giordano Vincenzo , Godoy-Santos Alexandre Leme , de Souza Felipe Serrão , Koch Hilton Augusto , de Cesar Netto Cesar , Rammelt Stefan
Published: November 2018

Avulsion fractures of the calcaneal tuberosity represent a rare injury pattern that is caused by a powerful tension force from the Achilles tendon and is usually seen following minor trauma, especially in elderly patients. The objective of this study is to describe a surgical technique using cerclage wiring through cannulated screws in the treatment of extra- and intra-articular avulsion fractures of the calcaneal tuberosity and to present our results in a small patient’s cohort. Through a 5.0 cm longitudinal skin incision over the posterolateral aspect of the calcaneus, after adequate debridement of the fracture fragments and while keeping the ankle in plantarflexion, the calcaneal tuberosity is anatomically reduced with the help of a periarticular reduction clamp and an accessory plantar longitudinal approach. Provisionally fixation is performed with K-wires. Definitive fixation is achieved with two parallel partially threaded 7.0 cannulated screws, which are positioned from the superior and posterior aspect of the tuberosity to the inferior and anterior aspect of the plantar surface of the calcaneus, and 1.5 mm cerclage wires that are pulled epiperiosteally to the plantar aspect of the calcaneus to avoid damage to local soft tissues. Alternatively, for smaller fracture fragments, two 3.5 mm partially threaded cannulated screws and 1.25 mm cerclage wires can be used. We also report the results of the procedure in a small cohort of four patients. All fractures healed in an anatomic position. There was no failure of fixation, loss of reduction, or need for secondary surgery, including hardware removal. At final follow-up, all patients had regained full plantar flexion range of motion and strength, with no gait or weight-bearing restrictions. In conclusion, the combination of cerclage wire and large diameter cannulated screws represents a promising option in the treatment of avulsion fractures of the calcaneal tuberosity, demonstrating good functional and radiographic results in our cohort of patients.
Effects of Intraoperative Dexamethasone and Ondansetron on Postoperative Nausea and Vomiting in Microvascular Decompression Surgery: A Randomized Controlled Study
Authors: Thongrong Cattleya , Chullabodhi Patitha , Kasemsiri Pornthep , Kitkhuandee Amnat , Plailaharn Narin , Sabangban Lumyai , Jimarsa Thirada
Published: November 2018

Background. Postoperative nausea and vomiting (PONV) is a common problem and may lead to catastrophic complications, especially in neurosurgical cases. The aim of this study was to evaluate the effects of dexamethasone and ondansetron for preventing PONV in patients who underwent microvascular decompression (MVD) surgery. Methods. A prospective, double-blinded, randomized control trial was conducted with 54 patients who underwent MVD. Patients were allocated into two groups. The study group (Gr. D) received intraoperative dexamethasone 4 mg iv and ondansetron 4 mg iv, whereas the control group (Gr. N) received placebo (0.9% normal saline 1 ml iv and 0.9% normal saline 2 ml iv). The incidence and severity of PONV were observed at 1, 2, 4, and 24 hr postsurgery. Results. At 1, 2, 4, and 24 hr postsurgery, Gr. D had a lower incidence (7.4%, 11.1%, 29.6%, and 66.7%) and lower severity of PONV than Gr. N (18.5%, 29.6%, 37.0%, and 81.5% at 1, 2, 4, and 24 hr; p>0.05). The requirement for antiemetic drugs was not significantly different between the groups (p>0.05). Conclusion. Administration of dexamethasone and ondansetron 4 mg seemed to decrease the incidence of PONV in the first 24 hours but not significantly. Therefore, further studies are to be carried out by escalating either dexamethasone dose or the dose of ondansetron or both.
Anaplastic Pleomorphic Xanthoastrocytoma Presenting with Musical Hallucination
Authors: Oladiran Oreoluwa , Nwosu Ifeanyi , Obanor Steve , Ogbonna-Nwosu Chinyere , Le Brian
Published: November 2018

Musical hallucinations are a relatively rare form of auditory hallucination characterized by hearing of music in the absence of any external stimuli. This phenomenon has been linked to both psychiatric and structural lesions. We present the case of a previously healthy young male whose presentation with musical hallucinations led to the diagnosis of a rare tumour, anaplastic pleomorphic xanthoastrocytoma.
Oxidative Stress in Autistic Children Alters Erythrocyte Shape in the Absence of Quantitative Protein Alterations and of Loss of Membrane Phospholipid Asymmetry
Authors: Bolotta Alessandra , Battistelli Michela , Falcieri Elisabetta , Ghezzo Alessandro , Manara Maria Cristina , Manfredini Stefano , Marini Marina , Posar Annio , Visconti Paola , Abruzzo Provvidenza Maria
Published: November 2018

Red blood cells (RBCs) from people affected by autism spectrum disorders (ASDs) are a target of oxidative stress. By scanning electron microscopy, we analyzed RBC morphology from 22 ASD children and show here that only 47.5 ± 3.33% of RBC displayed the typical biconcave shape, as opposed to 87.5 ± 1.3% (mean ± SD) of RBC from 21 sex‐ and age‐matched healthy typically developing (TD) controls. Codocytes and star‐shaped cells accounted for about 30% of all abnormally shaped ASD erythrocytes. RBC shape alterations were independent of the anticoagulant used (Na2‐EDTA or heparin) and of different handling procedures preceding glutaraldehyde fixation, thus suggesting that they were not artefactual. Incubation for 24 h in the presence of antioxidants restored normal morphology in most erythrocytes from ASD patients. By Coomassie staining, as well as Western blotting analysis of relevant proteins playing a key role in the membrane‐cytoskeleton organization, we were unable to find differences in RBC ghost composition between ASD and normal subjects. Phosphatidylserine (PS) exposure towards the extracellular membrane domain was examined in both basal and erythroptosis‐inducing conditions. No differences were found between ASD and TD samples except when the aminophospholipid translocase was blocked by N‐ethylmaleimide, upon which an increased amount of PS was found to face the outer membrane in RBC from ASD. These complex data are discussed in the light of the current understanding of the mode by which oxidative stress might affect erythrocyte shape in ASD and in other pathological conditions.
Opening the Blood-Brain Barrier and Improving the Efficacy of Temozolomide Treatments of Glioblastoma Using Pulsed, Focused Ultrasound with a Microbubble Contrast Agent
Authors: Dong Qian , He Lin , Chen Linbo , Deng Qiongzhen
Published: November 2018

Objective.To explore the effects of pulsed, focused, and microbubble contrast agent-enhanced ultrasonography (mCEUS) on blood-brain barrier (BBB) permeability and the efficacy temozolomide for glioblastoma.Methods.Wistar rats (n = 30) were divided into three groups (n = 10 per group) to determine optimal CUES conditions for achieving BBB permeability, as assessed by ultrastructure transmission electron microscopy (TEM) and western blot assays for the tight junction protein claudin-5. Optimized mCEUS effects on BBB permeability were subsequently confirmed with Evans blue staining (2 groups of 10 rats). The glioma cell line 9L was injected into the brain striatum of Wistar rats. After temozolomide chemotherapy, we detected glial fibrillary acidic protein (GFAP) levels in serum by enzyme-linked immunosorbent assay (ELISA) and in brain tissue by western blot, immunocytochemistry, and real-time quantitative polymerase chain reaction (qPCR).Results.BBB permeability was maximized with 1 ml/kg contrast agent mCEUS delivered via 10-min intermittent launches with a 400-ms interval. Evans blue staining confirmed BBB permeability following ultrasonic cavitation in the control group (P < 0.05). Following temozolomide chemotherapy, levels of the tumor marker GFAP were increased in the group with ultrasonic cavitation compared with the control group (P < 0.05).Conclusions.When rats were treated by mCEUS with intermittent launches (interval, 400 ms) and injected with 1 mg/kg contrast agent, BBB permeability was increased and temozolomide BBB penetration was enhanced, therapeutic enhancement for glioblastoma.
Photobiomodulation at Multiple Wavelengths Differentially Modulates Oxidative Stress In Vitro and In Vivo
Authors: Rupel Katia , Zupin Luisa , Colliva Andrea , Kamada Anselmo , Poropat Augusto , Ottaviani Giulia , Gobbo Margherita , Fanfoni Lidia , Gratton Rossella , Santoro Massimo , Di Lenarda Roberto , Biasotto Matteo , Zacchigna Serena
Published: November 2018

Photobiomodulation (PBM) is emerging as an effective strategy for the management of multiple inflammatory conditions, including oral mucositis (OM) in cancer patients who receive chemotherapy or radiotherapy. Still, the poor understanding of the mechanisms by which the light interacts with biological tissues and the heterogeneity of light sources and protocols employed worldwide significantly limits its applicability. Reactive oxygen species (ROS) are massively generated during the early phases of OM and play a major role in the pathogenesis of inflammation in general. Here, we report the results of a clinical and experimental study, aimed at evaluating the effect of laser light at different wavelengths on oxidative stress in vivo in oncologic patients suffering from OM and in vitro in two cell types abundantly present within the inflamed oral mucosa, neutrophil polymorphonuclear (PMN) granulocytes, and keratinocytes. In addition to standard ROS detection methods, we exploited a roGFP2‐Orp1 genetically encoded sensor, allowing specific, quantitative, and dynamic imaging of redox events in living cells in response to oxidative stress and PBM. We found that the various wavelengths differentially modulate ROS production. In particular, the 660 nm laser light increases ROS production when applied either before or after an oxidative stimulus. In contrast, the 970 nm laser light exerted a moderate antioxidant activity both in the saliva of OM patients and in both cell types. The most marked reduction in the levels of ROS was detected in cells exposed either to the 800 nm laser light or to the combination of the three wavelengths. Overall, our study demonstrates that PBM exerts different effects on the redox state of both PMNs and keratinocytes depending on the used wavelength and prompts the validation of a multiwavelength protocol in the clinical settings.
The Deformation Characteristics of Weak Foundation with High Back Siltation in the Immersed Tunnel
Authors: Yue Xiabing , Xie Yuan , Xie Yongli
Published: November 2018

The deformation characteristics of a weak foundation with high back siltation in an immersed tunnel lack empirical data; however, the calculation method and control of foundation settlements are highly important to tunnel design and construction. This paper takes a cross section of the natural foundation of an immersed tunnel in the Hong Kong‐Zhuhai‐Macao Bridge (HZMB) as the research object and conducts a centrifuge model test. The soil layer is divided, and the soil parameters are determined by the CPTU and the indoor dynamic three triaxial test. In consideration of the actual engineering scale and the ability of the centrifuge model test system, the similarity ratio of the model to the prototype is studied and determined. As for the immersed tube structure, the deformation characteristics are mainly studied. Therefore, the organic glass is selected as the model material by the similarity of the elastic modulus and the density. The characteristics of the resilience and recompression of the natural foundation of the immersed tunnel and the strain characteristics of the immersed tube structure are obtained by the analysis of the multiworking condition test data. Moreover, based on the actual engineering geological conditions of the subsea tunnel, a numerical calculation method is carried out to check the reliability of the centrifuge model test results. The results of the numerical calculation are consistent with the model test results.
Protective Effect of Sika Deer (Cervus nippon) Velvet Antler Extract against Cisplatin-Induced Kidney and Liver Injury in a Prostate Cancer PC-3 Cell Xenograft Model
Authors: Tang Yujiao , Fan Meiqi , Choi Young-Jin , Choi Eun-Ju , Moon Sang-Ho , Debnath Trishna , Yu Yonghai , Lee Il Nam , Kim Eun-Kyung
Published: November 2018

We previously discovered the antioxidant and antiprostate cancer effects of antler extract (AE), but whether it inhibits cisplatin- (Cis-) induced toxicity has not been investigated. In this study, the effect of AE on Cis-induced side effects in the kidney and liver using 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide-based cytotoxicity and cell cycle assays in prostate cancer PC-3 cells in vitro is investigated. Furthermore, we used a xenograft mouse model of the same cells to examine the in vivo effects and mechanisms of action. Cis and Cis + AE treatment attenuated prostate cancer cell growth by inducing apoptosis in vitro. Cis + AE stimulated cleaved caspases 3, 7, and 9 and polyadenosine diphosphate ribose polymerase expression. Cis + AE treatment for 1 week significantly increased the superoxide dismutase and catalase antioxidant activity while thiobarbituric acid reactive substances decreased. The histopathological damage and tumor necrosis factor-α, interleukin- (IL-) 1β and IL-6, cyclooxygenase-2, and inducible nitric oxide synthase expression in the kidney and liver tissue decreased. Therefore, AE likely possesses antiprostate cancer activity and inhibits Cis toxicity.
Curcumin Downregulates Human GM3 Synthase (hST3Gal V) Gene Expression with Autophagy Induction in Human Colon Carcinoma HCT116 Cells
Authors: Lee Miri , Choi Hyunju , Kim Kyoung-Sook , Kim Dong-Hyun , Kim Cheorl-Ho , Lee Young-Choon
Published: November 2018

Our recent report showed that curcumin, polyphenolic compound isolated from the herb Curcuma longa, upregulated the gene expression of human GD3 synthase (hST8Sia I) responsible for ganglioside GD3 synthesis with autophagy induction in human lung adenocarcinoma A549 cells. In this study, on the contrary to this finding, we demonstrated that curcumin downregulated the gene expression of human GM3 synthase (hST3Gal V) catalyzing ganglioside GM3 synthesis with autophagy induction in human colon carcinoma HCT116 cells. To clarify the mechanism leading to the downregulation of hST3Gal V gene expression in curcumin‐treated HCT116 cells, we analyzed the curcumin‐inducible promoter of the hST3Gal V gene by luciferase reporter assays. Promoter deletion analysis demonstrated that the ‐177 to ‐83 region, which includes putative binding sites for transcription factors NFY, CREB/ATF, SP1, EGR3, and MZF1, acts as the curcumin‐responsive promoter of the hST3Gal V gene. Site‐directed mutagenesis and chromatin immunoprecipitation analysis demonstrated that the CREB/ATF binding site at ‐143 is pivotal for curcumin‐induced downregulation of hST3Gal V gene in HCT116 cells. The transcriptional activation of hST3Gal V in HCT116 cells was significantly repressed by an inhibitor of AMP‐activated protein kinase (AMPK). These results suggest that AMPK signal pathway mediates hST3Gal V gene expression in HCT116 cells.
Stability and Hopf Bifurcation Analysis in a Delayed Myc/E2F/miR-17-92 Network Involving Interlinked Positive and Negative Feedback Loops
Authors: Wang Guiyuan , Yang Zhuoqin
Published: November 2018

MiR-17-92 plays an important role in regulating the levels of the Myc/E2F protein. In this paper, we consider a coupling network between Myc/E2F/miR-17-92 delayed negative feedback loop and Myc/E2F positive feedback loop described by a two-dimensional delay differential equation. Based on linear stability analysis and bifurcation theory, sufficient conditions for stability of equilibria and oscillatory behaviors via Hopf bifurcation are derived when choosing time delay as well as negative feedback strength associated with oscillations as bifurcation parameters, respectively. Furthermore, direction and stability of Hopf bifurcation of time delay are studied by using the normal form method and center manifold theorem. Finally, several numerical simulations are performed to verify the results we obtained.
Thermodynamic Analysis of a Solar Combined Ejector Absorption Cooling System
Authors: Sioud Doniazed , Garma Raoudha , Bellagi Ahmed
Published: November 2018

The objective of this paper is to investigate theoretically a solar driven 60 kW absorption cooling system. The system is constituted of a combined ejector single-effect absorption cycle coupled with a linear Fresnel solar concentrator and using water/lithium bromide as working fluid. The combined ejector single-effect absorption cycle exhibits high performances, almost equal to that of double-effect absorption device. However, higher driving heat temperatures are required than in the case of conventional single-effect machines. A mathematical model is set up to analyze the optical performance of the linear Fresnel concentrator. Simulations are carried out to study the overall system performance COPsystem and the performances of the combined absorption machine COPcycle for generator driving temperatures and pressures in the ranges 180°C – 210°C and 198 kPa – 270 kPa, respectively. Further, the effect of operating parameters such as the cooling medium and chilled water temperatures is investigated. A maximum cycle performance of 1.03 is found for a generator pressure of 272 kPa and chilled and cooling water temperatures of 7°C and 25°C, respectively. A case study is investigated for a typical summer Tunisian day, from 8:00 to 18:00. The effect of ambient temperature and solar radiation on cycle and system performances is simulated. The optical performances of the concentrator are also analyzed. Simulation results show that between 11:00 and 14:00 the collector efficiency is 0.61 and that the COPcycle reaches values always higher than 0.9 and the COPsystem is larger than 0.55. Globally the performances of the investigated cycle are similar to those of double-effect conventional absorption system.
Exploring Promotion Effect for FIT Policy of Solar PV Power Generation Based on Integrated ANP: Entropy Model
Authors: Yang Jing , Yang Changhui , Song Yiming , Wang Xiaojia
Published: November 2018

The photovoltaic (PV) industry is an important developmental direction of the global new energy industry, and it is also a strategic emerging industry of China with an international competitive advantage. China’s PV industry has developed rapidly in recent years, and the new installed capacity of PV power generation has been ranked first in the world for 5 years. This achievement is inseparable from the support of national policy, especially the Feed-in Tariff (FIT) policy of solar PV power generation, which is a primary policy of the PV industry. This paper is a study of preference elicitation, whose goal was to identify the driving factors behind historical PV policy in China, according to the opinion of a panel of experts. On this basis, this paper proposes an integrated ANP-Entropy model to evaluate the FIT policy of China in three dimensions: environmental, energy, and economic (3E). Research results show that the environmental goal is still the most significant to the development of the PV industry in China, followed by the energy and economic goals, which account for 0.405, 0.358, and 0.237, respectively. Compared with the conventional studies on energy policy evaluations, this paper integrates subjective (Analytic Network Process) and objective (Entropy Weight Method) evaluations, which makes the evaluation results more reasonable and reliable. Additionally, the 3E evaluation index system and the ANP-Entropy model proposed in this paper are also applicable to the evaluation of other renewable energy policies.
Impact of Ileal Transposition Surgical Intervention on Antioxidant Status Measured in Liver Tissue of Obese Zucker Rats (Crl:ZUC‐Leprfa)
Authors: Skrzep-Poloczek Bronisława , Stygar Dominika , Sawczyn Tomasz , Romuk Ewa , Chełmecka Elżbieta , Poloczek Jakub , Dulska Agnieszka , Kazura Wojciech , Karcz Konrad W.
Published: November 2018

Background. The main factor characteristic for low‐grade systemic inflammation typical for obesity is oxidative stress (OS). Reactive oxygen species (ROS) production is higher and more increased in time in the obese patients than in lean subjects. Aims. To assess the effect of ileal transposition (IT) and sham types of bariatric procedures on the antioxidative systems in the liver tissue of Zucker rats (Crl:ZUC Leprfa). Method. 21 animals were divided into the experimental groups: control group (n = 7), sham group (n = 7), and IT group (n = 7). Sham and IT animals underwent selected surgery. The concentration of total antioxidant capacity (TAC), total antioxidant status (TOS), and activity of glutathione reductase (glutathione‐disulfide reductase, GR, GSR), catalase (CAT), glutathione peroxidase (GPx), glutathione S‐transferase (GST), and total superoxide dismutase activity (SOD) were assessed in liver tissue 3 months after surgery. Results. IT procedure significantly increased TAC when compared to sham and the control group. Animals after IT showed higher levels of TOS when compared to sham procedure. The total amount of TOS was similar in IT and control groups. GPx activity was increased in the groups submitted to the sham and IT surgery in relation to control. GR and CAT activities were significantly higher after IT in comparison to control and sham procedures. Total SOD and MnSOD were significantly higher in sham‐operated animals in comparison to IT intervention and control groups. Conclusions. IT procedure had a positive impact on the diminishing of oxidative stress measured by TAC and TOS markers. The dynamic, adaptive, and protective mechanisms of enzymatic antioxidant systems were observed after the IT but not sham procedure. Nevertheless, 3 months after surgery, the midterm effect of bariatric surgery was observed, which might not fully balance the antioxidative response.
Cochlear Ossification in a Patient with Cogan’s Syndrome Undergoing Bilateral Cochlear Implantation
Authors: Cassis Adam M.
Published: November 2018

We present the case of a young female patient diagnosed with Cogan’s syndrome after the rapid onset of profond hearing and vestibular loss with concomitant eye symptoms. After appropriate medical treatment, her hearing did not respond and she underwent bilateral simultaneous cochlear implantation with findings of extensive cochlear ossification in both ears. The case and outcome are described in the body of the paper.
Evaluation of Two Different Solvents for Azolla pinnata Extracts on Chemical Compositions and Larvicidal Activity against Aedes albopictus (Diptera: Culicidae)
Authors: Ravi Rajiv , Husna Zulkrnin Nor Shaida , Rozhan Nurul Nadiah , Nik Yusoff Nik Raihan , Mat Rasat Mohd Sukhairi , Ahmad Muhammad Iqbal , Hamzah Zulhazman , Ishak Intan H. , Mohd Amin Mohamad Faiz
Published: November 2018

Limited success for Aedes control program has impelled the necessities for new insecticide search. Hence, alternative plant compounds may be competent to overcome the pesticide resistance problem and to lead a chemical-free environment. Following go-green conceptions, larvicidal effects of the Azolla pinnata extracts using methanol and acetone solvent against Aedes albopictus late 3rd instar larvae were evaluated. The A. pinnata fresh plant from Kuala Krai, Kelantan, Malaysia (5° 31′ N 102° 12′ E) was used for crude extraction with Soxhlet apparatus using methanol and acetone solvents. Next, larvicidal test following WHO guidelines was tested against late 3rd instar to early 4th instar larvae of Ae. albopictus mosquitoes. Meanwhile, the chemical composition of extracts and their structures have been identified using GCMS-QP2010 Ultra (Shimadzu) fitted with Rtx-5MS capillary column (30 m × 0.25 mm inner diameter, ×0.25 μm film thickness; maximum temperature, 370°C), coupled to QP2010 Ultra (Shimadzu) MS. Results of methanol solvent showed the highest larvicidal activity against late 3rd instar to early 4th instar Ae. albopictus larvae with LC50 and LC95 values of 867 ppm and 1293 ppm at 24 hours, respectively, and 647 ppm and 972 ppm at 48 hours, respectively. Meanwhile, acetone solvent compounds were recorded with LC50 and LC95 values of 1072 ppm and 1302 ppm at 24 hours, respectively, and 904 ppm and 1126 ppm at 48 hours, respectively. Finally, the chemical composition of A. pinnata plant extracts has been characterized for 35 active compounds from methanol solvent and 37 active compounds with acetone solvent. In conclusion, A. pinnata plant bioactive molecules are efficient and could be developed as an eco-friendly, “go-green” approach for mosquitoes' larvicidal control programs. Thus, our study suggests that future research can be conducted on A. pinnata bioactive ingredients against Ae. albopictus larvae in small-scale field trials as botanical insecticide for environmentally friendly approach.
A Case Study Analysis of Clothing Shopping Mall for Customer Design Participation Service and Development of Customer Editing User Interface
Authors: Yuan Ying , Huh Jun-Ho
Published: November 2018

Following the development of networking and mobile devices, the technology of managing the offline information online is being conducted widely. Also, as the social services have become much more active, users are registering and managing their personal information on online websites and sharing it with other users to acquire the information they need. For modern people living in a smart city, the planning of smarter services is required. The convergence of ET and IT or advanced scientific technologies such as AI or Big Data is often mentioned whenever the smart city is discussed. Nevertheless, smart services that could introduce smart solutions to conventional industries or change existing lifestyles should also be considered. Therefore, this paper discusses a service related to the convergence of the traditional clothing industry with IT and a service wherein CT is converged with systems that allow customers to participate in the design work and share the designs they have created. In other words, this study is a case study of CT and IT services in the clothing industry and is inclusive of an apparel shopping mall service that encourages customer participation in design, a customer-oriented editing user interface, and a copyright management system. The results show that both production method and production capacity largely affect the user interface of apparel platform services, with customer freedom significantly correlated with their functional roles. Moreover, the lead index is shown to be one of the factors restraining customer freedom. With this analysis, an apparel shopping mall wherein customers participate in the design work has been developed especially for clothes with more complex designs. The shopping mall emphasizes functionality from the perspective of customer use. At the same time, an online environment for an apparel service appropriate for the smart city has been implemented.
Particle Filter and Finite Impulse Response Filter Fusion and Hector SLAM to Improve the Performance of Robot Positioning
Authors: Bassiri Amin , Asghari Oskoei Mohammadreza , Basiri Anahid
Published: November 2018

Indoor position estimation is essential for navigation; however, it is a challenging task mainly due to the indoor environments’ (a) high noise to signal ratio and (b) low sampling rate and (c) sudden changes to the environments. This paper uses a hybrid filter algorithm for the indoor positioning system for robot navigation integrating Particle Filter (PF) algorithm and Finite Impulse Response (FIR) filter algorithm to assure the continuity of the positioning solution. Additionally, the Hector Simultaneous Localisation and Mapping (Hector SLAM) algorithm is used to map the environment and improve the accuracy of the navigation. The paper implements the hybrid algorithm that uses the integrated PF, FIR, and Hector SLAM, using an embedded laser scanner sensor. The hybrid algorithm coupled with Hector SLAM is tested in several scenarios to evaluate the performance of the system, in terms of continuity and accuracy of the position estimation, and compares it with similar systems. The scenarios where the system is tested include reducing the laser sensor readings (low sampling rate), dynamic environments (change in the location of the obstacles), and the kidnapped robot situation. The results show that the system provides a significantly better accuracy and continuity of the position estimation in all scenarios, even in comparison with similar hybrid systems, except where there is a high and constant noise, where the performance of the hybrid filter and the simple PF seems almost the same.
Nephrectomy for Metastatic Kidney Tumor in Patients with Differentiated Thyroid Cancer: A Report of Two Cases
Authors: Yamazaki Haruhiko , Kishida Takeshi , Noguchi Go , Iwasaki Hiroyuki , Suganuma Nobuyasu , Masudo Katsuhiko , Nakayama Hirotaka , Yamashita Toshinari , Yamanaka Takashi , Sugawara Yuko , Matsubara Yuka , Kohagura Kaori , Rino Yasushi , Masuda Munetaka
Published: November 2018

The occurrence of renal tumors originating from thyroid cancer is extremely rare with a few effective treatments for renal metastases. Here, we report the cases of two patients with differentiated thyroid cancer who underwent nephrectomy for a metastatic kidney tumor. Case 1 was a 74-year-old man who was diagnosed with right kidney tumor 10 years after initial surgery for papillary thyroid cancer (PTC). Right nephrectomy was performed, and the pathology was metastatic PTC. Case 2 was a 68-year-old woman who was diagnosed with left kidney tumor 24 years after surgery for follicular thyroid carcinoma (FTC). Left nephrectomy was performed, and the pathology was metastatic FTC. Nephrectomy for single renal metastasis could be considered a treatment option if the patients’ general condition is positive.
Endothelial Function in Children with Acute Lymphoblastic Leukemia (ALL) May Reflect the Clinical Outcome
Authors: Doroszko Adrian , Niedzielska Ewa , Jakubowski Maciej , Porwolik Julita , Turek-Jakubowska Aleksandra , Szahidewicz-Krupska Ewa , Sieczkowski Bartosz , Dobrowolski Piotr , Radziwon Aneta , Skomro Robert , Derkacz Arkadiusz , Mazur Grzegorz , Chybicka Alicja , Szuba Andrzej
Published: November 2018

Endothelial dysfunction is a common feature of early complications of hemato-oncologic therapy. The aim of our study was to assess the profile of endothelial function at diagnosis time, then during initial treatment phase of acute lymphoblastic leukemia (ALL), and to verify the presence of its correlation with early clinical outcome (ECO). 28 ALL children and 18 healthy age-matched control ones were recruited. Study group was examined at baseline and at 33rd and 78th day of treatment. At each protocol step the endothelial function was assessed by measurement of sP-selectin (CD62-P), PAI-1(serpinE1), sE-selectin (CD62E), sICAM-1(sCD54), sVCAM-1(sCD106), and VEGF concentrations. Higher baseline sICAM-1 and sVCAM-1 levels and lower sP-selectin and VEGF were observed in children with ALL. sICAM-1, sVCAM-1, and sE-selectin levels were decreasing following the treatment with protocol I. Higher sE-selectin and lower baseline sICAM-1 levels were observed in children treated unsuccessfully. Lower PAI-1 levels were observed in children who survived. Higher baseline sE-selectin levels and lower sICAM-1 and VEGF were observed in children treated unsuccessfully. A decrease in sE-selectin and lower PAI-1 at the 78th day of therapy were associated with better ECO. High baseline VEGF and sE-selectin levels, significant increase in PAI-1, and low initial sICAM-1 levels are prognostics for poorer prognosis in the ALL children.
Understanding the Multitarget Pharmacological Mechanism of the Traditional Mongolian Common Herb Pair GuangZao‐RouDouKou Acting on Coronary Heart Disease Based on a Bioinformatics Approach
Authors: Lu Jingkun , Hu Yuchong , Wang Lechun , Wang Yuewu , Na Shengsang , Wang Jian , Shun Yuhui , Wang Xiaoqin , Xue Peifeng , Zhao Pengwei , Su Liping
Published: November 2018

GuangZao and RouDouKou (Fructus Choerospondiatis and Nutmeg, FCN) are one of the most common herb pairs in traditional Mongolian medicine for the treatment of coronary heart disease (CHD). However, evidence for the protective effect of FCN is limited, and its underlying mechanism of action remains unclear. The present study employed a network pharmacology approach to identify the potentially active ingredients and synergistic effects of the herb pair FCN as traditional Mongolian medicine. We predicted the targets of all available FCN ingredients with PharmMapper, SWISS, and SuperPred Server and clustered CHD‐related targets from the DrugBank and the OMIM database. We also evaluated the links between herbal ingredients and pharmacological actions to explore the potential mechanism of action of FCN. We found that FCN targets a network of CHD‐related key processes, including stress responses, cell adhesion and connections, angiogenesis, cell apoptosis and necrosis, the endocrine system, inflammatory and immune responses, and other biological processes. To confirm the predicted results, we investigated the protective effect of FCN on isoproterenol‐ (ISO‐) induced myocardial ischemia in rats. Pathological assessment indicated that FCN inhibits apoptosis and inflammatory responses involving the myocardium. Quantitative real‐time polymerase chain reaction (qRT‐PCR) and western blotting analyses demonstrated the therapeutic effects of FCN on ISO‐induced myocardial ischemia rats, possibly via regulating stress and inflammatory responses and inhibiting cardiomyocyte apoptosis. The findings of the present study indicate that bioinformatics combined with experimental verification provide a credible and objective method to elucidate the complex multitarget mechanism of action of FCN.
Long-Term Supplementation of Laying Hen Diets with Various Selenium Sources as a Method for the Fortification of Eggs with Selenium
Authors: Słupczyńska Maja , Jamroz Dorota , Orda Janusz , Wiliczkiewicz Andrzej , Król Barbara
Published: November 2018

The following study focuses on a comparison of the effectiveness of egg content enrichment with selenium (Se) via application of sodium selenite (Na-selenite), selenium-enriched yeast (Se-Yeast), or selenomethionine (Se-Met) in laying hen diets. Two hundred sixteen laying hens were divided into four treatments, each comprising eighteen replications, and each with three hens per cage. Animals were fed a basal diet without Se supplementation (control: selenium content 0.058 mg/kg), with the addition of Na-selenite, Se-Yeast, or Se-Met in amounts equivalent to 0.3 mg/kg of added selenium. The egg quality, the selenium content in eggs after the third and the fifth months of using Se supplementation, and the selenium level in the liver were determined. Enrichment of egg content with selenium was the most effective (382 μg/kg) via application of dietary Se-Met. Application of Na-selenite and Se-Yeast led to a similar effect on Se-accretion in egg content (255.9 and 258.9 μg/kg, respectively). Additionally, the calculated average Se concentration in one fresh egg was also higher in eggs from hens that received selenium additives in their diet and was far higher, almost three times higher for Se-Met addition, than the concentrations in controls. Se-accretion in the liver wet tissue was greater following application of Se-Yeast in the diet than following other treatments. These results indicate that the use of selenomethionine in the laying hen diet is the best method of enriching eggs with this micronutrient. In turn, the eggs obtained in this way can be an excellent source of highly bioavailable selenium in the human diet.
Three-Dimensional Morphological Characteristics of Lower Lumbar Intervertebral Foramen with Age
Authors: Yan Shuaifeng , Zhang Yunfan , Wang Kai , Han Yingchao , Zhu Kai , He Fan , Tan Jun
Published: November 2018

Intervertebral foramen is the doorway of nerve root and it plays an important role of radiculopathy and surgical treatment of intervertebral foramen diseases. The purpose of the study is to obtain three-dimensional (3D) morphological characteristics of lumbar intervertebral foramen and their relationship with age. Pedicle-superior articular process (P-SAP), disc height between adjacent vertebra (DH), pedicle-inferior vertebrae (P-IV), inferior posterior vertebrae-superior articular process (IPV-SAP), and bony boundary area (BBA) were measured in entrance, middle slice, and exit of lumbar intervertebral foramen for 25 males of different age groups. Spinous process to intervertebral foramen entrance (SP-IFE) was measured for 25 males of different age groups. Overall, P-SAP and P-IV decreased and IPV-SAP increased from the entrance to the exit of intervertebral foramen for L3/4-L5S1. DH decreased at entrance slice, middle slice, and exit slice for L3/4-L5S1 with age. Significant difference with aging was found only at the middle slice of L3/4 and L4/5 for P-SAP. And the significant decrease of IPV-SAP was observed at middle slice of L3/4, entrance slice of L4/5 and L5S1, and exit slice of L5S1. SP-IFE is not consistent for all subjects. In addition, the decrease of BBA at L3/4 and L4/5 was observed earlier than at L5S1. The present study described detailed information of intervertebral foramen, which may be of benefit for better understanding of the pathology and surgical planning for intervertebral foramen diseases.
Additive Manufacturing of Cobalt‐Based Dental Alloys: Analysis of Microstructure and Physicomechanical Properties
Authors: Hitzler Leonhard , Alifui-Segbaya Frank , Williams Philipp , Heine Burkhard , Heitzmann Michael , Hall Wayne , Merkel Markus , Öchsner Andreas
Published: November 2018

The limitations of investment casting of cobalt‐based alloys are claimed to be less problematic with significant improvements in metal additive manufacturing by selective laser melting (SLM). Despite these advantages, the metallic devices are likely to display mechanical anisotropy in relation to build orientations, which could consequently affect their performance “in vivo.” In addition, there is inconclusive evidence concerning the requisite composition and postprocessing steps (e.g., heat treatment to relieve stress) that must be completed prior to using the devices. In the current paper, we evaluate the microstructure of ternary cobalt‐chromium‐molybdenum (Co‐Cr‐Mo) and cobalt‐chromium‐tungsten (Co‐Cr‐W) alloys built with direct metal printing and LaserCUSING SLM systems, respectively, at 0°, 30°, 60°, and 90° inclinations (Φ) in as‐built (AB) and heat‐treated (HT) conditions. The study also examines the tensile properties (Young’s modulus, E; yield strength, RP0.2; elongation at failure, At; and ultimate tensile strength, Rm), relative density (RD), and microhardness (HV5) and macrohardness (HV20) as relevant physicomechanical properties of the alloys. Data obtained indicate improved tensile properties and HV values after a short and cost‐effective heat‐treatment cycle of Co‐Cr‐Mo alloys; however, the process did not homogenize the microstructure of the alloy. Annealing heat treatment of Co‐Cr‐W led to significant isotropic characteristics with increased E and At (except for Φ = 90°) in contrast to decreased RP0.2, Rm, and HV values, compared to the AB form. Similarly, the interlaced weld‐bead structures in AB Co‐Cr‐W were removed during heat treatment, which led to a complete recrystallization of the microstructure. Both alloys exhibited defect‐free microstructures with RD exceeding 99.5%.