bluuebunny/crossref_metadata_2025 huggingface dataset:
Origanum dubium (Cypriot Oregano) as a Promising Sanitizing Agent against Salmonella enterica and Listeria monocytogenes on Tomato and Cucumber Fruits
Authors: Xylia Panayiota , Chrysargyris Antonios , Miltiadous Panagiota , Tzortzakis Nikolaos
Published: December 2022

In recent years, the use of natural products such as essential oils (EOs) and other plant extracts for the preservation of fresh produce has attracted much interest from the food industry. Many endemic medicinal and aromatic plants, such as Cypriot oregano (Origanum dubium), present a plethora of properties that can be utilized by the fruit and vegetable sectors of the food industry. The purpose of the present study was to assess the effects of O. dubium EO and hydrosol (at different concentrations and durations of dipping application) for the preservation of tomato and cucumber fruit quality, and their effectiveness as sanitizing agents against two foodborne pathogens (Listeria monocytogenes and Salmonella enterica). The results of this study indicated that increased concentrations of EO, combined with a longer duration of application, resulted in less marketable fruit compared to hydrosol application. Interestingly, EO application at lower concentrations and shorter durations of application (i.e., 0.01% for 5 min) increased fruit antioxidant, ascorbic acid and carotenoid levels (for tomato fruit), suggesting an increase in the nutritional value of the treated fruit, compared to the control. EO and hydrosol were able to decrease the bacterial populations (both bacteria) on fruits. Both products were especially effective against L. monocytogenes, even seven days after their application and storage at 11 °C (up to an approx. 3 log reduction with the EO application). Overall, the results of this study suggest that the use of O. dubium EO and hydrosol could be considered as alternative sanitation means for tomatoes and cucumbers.
Chrysanthemum: A Comprehensive Review on Recent Developments on In Vitro Regeneration
Authors: Eisa Eman Abdelhakim , Tilly-Mándy Andrea , Honfi Péter , Shala Awad Yousef , Gururani Mayank Anand
Published: December 2022

Chrysanthemum is a flowering plant grown worldwide and is one of the most popular ornamental plants. Chrysanthemums are usually cultivated using root suckers and shoot cuttings. This conventional technique is relatively slow. In addition, as cuttings are gained regularly from mother plants, there is a chance of viral infection and degeneration, which raises the production cost. The hurdles mentioned above have been managed by applying in vitro propagation techniques, which can enhance reproduction rates through in vitro culture and use very small explants, which are impossible with the conventional approach. Usually, it is difficult to get true-to-type plants as the parents with good quality, but clonal propagation of a designated elite species makes it possible. Hence, this review highlights recent studies of the in vitro propagation of Chrysanthemum included; the appropriate explant sources, medium compositions, alternative disinfection of culture media, plant growth regulators (PGRs), different mutagenesis applications, acclimatization efficiency, and alternative light sources to overcome the shortcomings of conventional propagation techniques.
Comprehensive Genomic Profiling of Cell-Free Circulating Tumor DNA Detects Response to Ribociclib Plus Letrozole in a Patient with Metastatic Breast Cancer
Authors: Silveira Catarina , Sousa Ana Carla , Corredeira Patrícia , Martins Marta , Sousa Ana Rita , Da Cruz Paula Arnaud , Selenica Pier , Brown David N. , Golkaram Mahdi , Kaplan Shannon , Zhang Shile , Liu Li , Weigelt Britta , Reis-Filho Jorge S. , Costa Luís , Carmo-Fonseca Maria
Published: December 2022

Analysis of cell-free circulating tumor DNA obtained by liquid biopsy is a non-invasive approach that may provide clinically actionable information when conventional tissue biopsy is inaccessible or infeasible. Here, we followed a patient with hormone receptor-positive and human epidermal growth factor receptor (HER) 2-negative breast cancer who developed bone metastases seven years after mastectomy. We analyzed circulating cell-free DNA (cfDNA) extracted from plasma using high-depth massively parallel sequencing targeting 468 cancer-associated genes, and we identified a clonal hotspot missense mutation in the PIK3CA gene (3:178952085, A > G, H1047R) and amplification of the CCND1 gene. Whole-exome sequencing revealed that both alterations were present in the primary tumor. After treatment with ribociclib plus letrozole, the genetic abnormalities were no longer detected in cfDNA. These results underscore the clinical utility of combining liquid biopsy and comprehensive genomic profiling to monitor treatment response in patients with metastasized breast cancer.
Structural Basis of Botulinum Toxin Type F Binding to Glycosylated Human SV2A: In Silico Studies at the Periphery of a Lipid Raft
Authors: Azzaz Fodil , Hilaire Didier , Fantini Jacques
Published: December 2022

Botulinum neurotoxins are the deadliest microbial neurotoxins in humans, with a lethal dose of 1 ng/kg. Incidentally, these neurotoxins are also widely used for medical and cosmetic purposes. However, little is known about the molecular mechanisms that control binding of botulinum neurotoxin type F1 (BoNT/F1) to its membrane receptor, glycosylated human synaptic vesicle glycoprotein A (hSV2Ag). To elucidate these mechanisms, we performed a molecular dynamics simulation (MDS) study of initial binding kinetics of BoNT/F1 to SV2A. Since this toxin also interacts with gangliosides, the simulations were performed at the periphery of a lipid raft in the presence of both SV2A and gangliosides. Our study suggested that interaction of BoNT/F1 with SV2A is exclusively mediated by N-glycan moiety of SV2A, which interacts with aromatic residues Y898, Y910, F946, Y1059 and H1273 of this toxin. Thus, in contrast with botulinum neurotoxin A1 (BoNT/A1), BoNT/F1 does not interact with protein content of SV2A. We attributed this incapability to a barrage effect exerted by neurotoxin residues Y1132, Q1133 and K1134, which prevent formation of long-lasting intermolecular hydrogen bonds. We also provided structural elements that suggest that BoNT/F1 uses the strategy of BoNT/A1 combined with the strategy of botulinum neurotoxin type E to bind N-glycan of its glycoprotein receptor. Overall, our study opened a gate for design of a universal inhibitor aimed at disrupting N-glycan–toxin interactions and for bioengineering of a BoNT/F1 protein that may be able to bind protein content of synaptic vesicle glycoprotein for therapeutic purposes.
Gut Microbiota Alterations in Trace Amine-Associated Receptor 9 (TAAR9) Knockout Rats
Authors: Zhukov Ilya S. , Vaganova Anastasia N. , Murtazina Ramilya Z. , Alferova Lyubov S. , Ermolenko Elena I. , Gainetdinov Raul R.
Published: December 2022

Trace amine-associated receptors (TAAR1-TAAR9) are a family of G-protein-coupled monoaminergic receptors which might have great pharmacological potential. It has now been well established that TAAR1 plays an important role in the central nervous system. Interestingly, deletion of TAAR9 in rats leads to alterations in the periphery. Previously, we found that knockout of TAAR9 in rats (TAAR9-KO rats) decreased low-density lipoprotein cholesterol levels in the blood. TAAR9 was also identified in intestinal tissues, and it is known that it responds to polyamines. To elucidate the role of TAAR9 in the intestinal epithelium, we analyzed TAAR9-co-expressed gene clusters in public data for cecum samples. As identified by gene ontology enrichment analysis, in the intestine, TAAR9 is co-expressed with genes involved in intestinal mucosa homeostasis and function, including cell organization, differentiation, and death. Additionally, TAAR9 was co-expressed with genes implicated in dopamine signaling, which may suggest a role for this receptor in the regulation of peripheral dopaminergic transmission. To further investigate how TAAR9 might be involved in colonic mucosal homeostasis, we analyzed the fecal microbiome composition in TAAR9-KO rats and their wild-type littermates. We identified a significant difference in the number of observed taxa between the microbiome of TAAR9-KO and wild-type rats. In TAAR9-KO rats, the gut microbial community became more variable compared with the wild-type rats. Furthermore, it was found that the family Saccharimonadaceae, which is one of the top 10 most abundant families in TAAR9-KO rat feces, is almost completely absent in wild-type animal fecal samples. Taken together, these data indicate a role of TAAR9 in intestinal function.
Transcriptomics and Metabolomics of Reactive Oxygen Species Modulation in Near-Null Magnetic Field-Induced Arabidopsis thaliana
Authors: Parmagnani Ambra S. , Mannino Giuseppe , Maffei Massimo E.
Published: December 2022

The geomagnetic field (GMF) is a natural component of Earth’s biosphere. GMF reduction to near-null values (NNMF) induces gene expression modulation that generates biomolecular, morphological, and developmental changes. Here, we evaluate the effect of NNMF on gene expression and reactive oxygen species (ROS) production in time-course experiments on Arabidopsis thaliana. Plants exposed to NNMF in a triaxial Helmholtz coils system were sampled from 10 min to 96 h to evaluate differentially expressed genes (DEGs) of oxidative stress responses by gene microarray. In 24–96 h developing stages, H2O2 and polyphenols were also analyzed from roots and shoots. A total of 194 DEGs involved in oxidative reactions were selected, many of which showed a fold change ≥±2 in at least one timing point. Heatmap clustering showed DEGs both between roots/shoots and among the different time points. NNMF induced a lower H2O2 than GMF, in agreement with the expression of ROS-related genes. Forty-four polyphenols were identified, the content of which progressively decreased during NNMF exposition time. The comparison between polyphenols content and DEGs showed overlapping patterns. These results indicate that GMF reduction induces metabolomic and transcriptomic modulation of ROS-scavenging enzymes and H2O2 production in A. thaliana, which is paralleled by the regulation of antioxidant polyphenols.
Designing a Clinical Trial with Olfactory Ensheathing Cell Transplantation-Based Therapy for Spinal Cord Injury: A Position Paper
Authors: Reshamwala Ronak , Murtaza Mariyam , Chen Mo , Shah Megha , Ekberg Jenny , Palipana Dinesh , Vial Marie-Laure , McMonagle Brent , St John James
Published: December 2022

Spinal cord injury (SCI) represents an urgent unmet need for clinical reparative therapy due to its largely irreversible and devastating effects on patients, and the tremendous socioeconomic burden to the community. While different approaches are being explored, therapy to restore the lost function remains unavailable. Olfactory ensheathing cell (OEC) transplantation is a promising approach in terms of feasibility, safety, and limited efficacy; however, high variability in reported clinical outcomes prevent its translation despite several clinical trials. The aims of this position paper are to present an in-depth analysis of previous OEC transplantation-based clinical trials, identify existing challenges and gaps, and finally propose strategies to improve standardization of OEC therapies. We have reviewed the study design and protocols of clinical trials using OEC transplantation for SCI repair to investigate how and why the outcomes show variability. With this knowledge and our experience as a team of biologists and clinicians with active experience in the field of OEC research, we provide recommendations regarding cell source, cell purity and characterisation, transplantation dosage and format, and rehabilitation. Ultimately, this position paper is intended to serve as a roadmap to design an effective clinical trial with OEC transplantation-based therapy for SCI repair.
The High-Resolution Structure Reveals Remarkable Similarity in PD-1 Binding of Cemiplimab and Dostarlimab, the FDA-Approved Antibodies for Cancer Immunotherapy
Authors: Jeong Tae-Jun , Lee Hyun-Tae , Gu Nahyeon , Jang Yu-Jeong , Choi Seung-Beom , Park Ui-Beom , Lee Sang-Hyung , Heo Yong-Seok
Published: December 2022

Multiple tumors have responded well to immunotherapies, which use monoclonal antibodies to block the immune checkpoint proteins and reactivate the T-cell immune response to cancer cells. Significantly, the anti-PD-1 antibodies pembrolizumab and nivolumab, which were approved in 2014, have revolutionized cancer therapy, demonstrating dramatic improvement and longer duration. The US FDA authorized the third anti-PD-1 medication, cemiplimab, in 2018 for use in patients with cutaneous squamous cell carcinoma. To further understand the molecular mechanism of the antibody drug, we now reveal the intricate structure of PD-1 in complex with the cemiplimab Fab at a resolution of 1.98 Å. The cemiplimab–PD-1 interaction preoccupies the space for PD-L1 binding with a greater binding affinity than the PD-1/PD-L1 interaction, which is the basis for the PD-1 blocking mechanism. The structure reveals that cemiplimab and dostarlimab are significantly similar in PD-1 binding, although the precise interactions differ. A comparative investigation of PD-1 interactions with the four FDA-approved antibodies reveals that the BC, C’D, and FG loops of PD-1 adopt distinct conformations for optimal interaction with the antibodies. The structural characteristics in this work could be helpful information for developing more potent anti-PD-1 biologics against cancer.
The Anti-Inflammatory Effects of Iberin on TNF-α-Stimulated Human Oral Epithelial Cells: In Vitro Research
Authors: Hosokawa Yoshitaka , Hosokawa Ikuko , Shimoyama Masahiro , Fujii Ayumi , Sato Juri , Kadena Kimitake , Ozaki Kazumi , Hosaka Keiichi
Published: December 2022

Iberin is a bioactive chemical found in cruciferous plants that has been demonstrated to have anticancer properties. However, there have been no reports on its effects on periodontal resident cells, and many questions remain unanswered. The aim of this study was to examine whether iberin had anti-inflammatory effects on human oral epithelial cells, including influences on signal transduction pathway activation in TNF-α-stimulated TR146 cells. Iberin inhibited the production of interleukin (IL)-6 and C-X-C motif chemokine ligand 10 (CXCL10), as well as the expression of vascular cell adhesion molecule (VCAM)-1, inducible nitric oxide synthase (iNOS), and cyclooxygenase (COX)-2 in tumor necrosis factor (TNF)-α-stimulated TR146 cells, a human oral epithelial cell line. Moreover, iberin administration increased the expression of antioxidant signaling pathways, such as Heme Oxygenase (HO)-1 and NAD(P)H quinone dehydrogenase 1 (NQO1). Furthermore, we found that iberin could inhibit the activation of the nuclear factor (NF)-κB, signal transducer and activator of transcription (STAT)3, and p70S6 kinase (p70S6K)-S6 ribosomal protein (S6) pathways in TNF-α-stimulated TR146 cells. In conclusion, iberin reduced inflammatory mediator expression in human oral epithelial cells by preventing the activation of particular signal transduction pathways.
Lipid Nanoparticles and Liposomes for Bone Diseases Treatment
Authors: Burdușel Alexandra-Cristina , Andronescu Ecaterina
Published: December 2022

Because of their outstanding biocompatibility, sufficient capacity to control drug release, and passive targeting capability, lipid nanoparticles are one of the world’s most widely utilized drug delivery systems. However, numerous disadvantages limit the use of lipid nanoparticles in clinical settings, especially in bone regeneration, such as challenges in transporting, storing, and maintaining drug concentration in the local area. Scaffolds are frequently employed as implants to provide mechanical support to the damaged area or as diagnostic and imaging tools. On the other hand, unmodified scaffolds have limited powers in fostering tissue regeneration and curing illnesses. Liposomes offer a solid foundation for the long-term development of various commercial solutions for the effective drug delivery-assisted treatment of medical conditions. As drug delivery vehicles in medicine, adjuvants in vaccination, signal enhancers/carriers in medical diagnostics and analytical biochemistry, solubilizers for various ingredients as well as support matrices for various ingredients, and penetration enhancers in cosmetics are just a few of the industrial applications for liposomes. This review introduces and discusses the use of lipid nanoparticles and liposomes and the application of lipid nanoparticles and liposome systems based on different active substances in bone diseases.
Efficacy and Safety of TiNO-Coated Stents versus Drug-Eluting Stents in Acute Coronary Syndrome: Systematic Literature Review and Meta-Analysis
Authors: Daoud Frederic C. , Létinier Louis , Moore Nicholas , Coste Pierre , Karjalainen Pasi P.
Published: December 2022

(1) Background: Practice guidelines define drug-eluting stents (DES) as the standard of care in coronary percutaneous coronary intervention (PCI), including in acute coronary syndrome (ACS). This is based on comparisons with bare-metal stents (BMS). However, non-drug-eluting titanium-nitride-oxide-coated stents (TiNOS) have not been taken into account. The objective of this study is to determine whether TiNOS can be used as an alternative to DES in ACS. (2) Methods: A prospective systematic literature review (SLR), conducted according to the PRISMA guidelines, was performed, wherein multiple literature databases from 2018 and 2022 were searched. Prospective, randomised, controlled trials comparing outcomes after PCI with TiNOS vs. DES in any coronary artery disease (CAD) were searched. Clinical outcomes were meta-analytic pooled risk ratios (RR) of device-oriented Major Adverse Cardiac Events (MACE) and their components. The analysis stratified outcomes reported with ACS-only vs. ACS jointly with chronic coronary syndrome (CCS). (3) Results: Five RCTs were eligible, comprising 1855 patients with TiNOS vs. 1363 with DES at a 1-year follow-up. Three enrolled patients presented with ACS only and two with ACS or CCS. The latter accounted for most of the patients. The one-year pooled RRs in those three RCTs were as follows: MACE 0.93 [0.72, 1.20], recurrent myocardial infarction (MI) 0.48 [0.31, 0.73], cardiac death (CD) 0.66 [0.33, 1.31], clinically driven target lesion revascularization (TLR) 1.55 [1.10, 2.19], and stent thrombosis (ST) 0.35 [0.20, 0.64]. Those results were robust to a sensitivity analysis. The evidence certainty was high in MACE and moderate or low in the other endpoints. (4) Conclusions: TiNOS are a non-inferior and safe alternative to DES in patients with ACS.
Dynamics of Lipid Profile in Antiretroviral-Naïve HIV-Infected Patients, Treated with TAF-Based Regimens: A Multicenter Observational Study
Authors: Martini Salvatore , Maggi Paolo , Gervasoni Cristina , Onorato Lorenzo , Ferrara Sergio , Alessio Loredana , Bellacosa Chiara , Esposito Vincenzo , Di Filippo Giovanni , Masiello Addolorata , Maddaloni Adelaide , Madonia Simona , D’Alessio Giovanna , Rizzo Viviana , Coppola Nicola
Published: December 2022

Background: The introduction of tenofovir alafenamide (TAF) in antiretroviral therapy has deeply modified the choice of the backbone for different treatment regimens, allowing the prevention of the bone and renal toxicity that was related to the previous formulation of tenofovir disoproxil fumarate (TDF). At the same time, literature data show an onset of dyslipidemia after a switch from TDF to TAF. To better understand the possible role of TAF in dyslipidemia, antiretroviral-naïve HIV-infected patients were evaluated, comparing those treated with TAF/emtricitabine with those with abacavir/lamivudine. Methods: We enrolled 270 antiretroviral-naïve HIV-infected patients in an observational, retrospective, longitudinal, multicenter study; they started treatment from 2017 to 2019 and were followed up for at least 72 weeks. We divided patients into two groups, one treated with a TAF-based backbone in their antiretroviral regimens (TAF group) and one without TAF (NO TAF group), to evaluate possible differences in the dynamics of lipid profiles from baseline(T0) to week 24 (T24), 48 (T48) and 72 (T72). Results: No significant differences were observed at baseline between the 2 groups. In the TAF group we observed a significant development of hypercholesterolemia throughout the follow-up (p < 0.0001), not evident in the NO TAF group, that instead showed a significant increase in high-density lipoprotein (HDL). There were no significant differences between the two groups regarding triglycerides, low-density lipoprotein (LDL) and cardiovascular risk index (CRI). A cholesterol-lowering treatment with statin, finally, was prescribed in 6 patients in both groups during the study. At binary logistic regression analysis, no factor was independently associated with hypercholesterolemia, except for higher age at T0. Conclusions: This real-life study shows that in HIV-naïve patients, TAF was associated with hypercholesterolemia throughout the follow-up. The clinical significance of this hypercholesterolemia will have to be clarified in further studies.
Stabilization of Copper-Based Biochips with Alumina for Biosensing Application
Authors: Beydoun Nour , Niberon Yann , Arnaud Laurent , Proust Julien , Nomenyo Komla , Zeng Shuwen , Lerondel Gilles , Bruyant Aurelien
Published: December 2022

Surface plasmon resonance devices typically rely on the use of gold-coated surfaces, but the use of more abundant metals is desirable for the long-term development of plasmonic biochips. As a substitute for gold, thin copper films have been deposited on glass coverslips by thermal evaporation. As expected, these films immersed in a water solution initially exhibit an intense plasmonic resonance comparable to gold. However, without protection, an angle-resolved optical analysis shows a rapid degradation of the copper, characterized by a continuous angular shift of the plasmonic resonance curve. We show that copper films protected with a thin layer of aluminum oxide of a few nanometers can limit the oxidation rate for a sufficient time to perform some standard measurements. As the process is simple and compatible with the current biochip production technique, such an approach could pave the way for the production of alternative and more sustainable biochips.
Progress of Enzymatic and Non-Enzymatic Electrochemical Glucose Biosensor Based on Nanomaterial-Modified Electrode
Authors: Mohamad Nor Noorhashimah , Ridhuan Nur Syafinaz , Abdul Razak Khairunisak
Published: December 2022

This review covers the progress of nanomaterial-modified electrodes for enzymatic and non-enzymatic glucose biosensors. Fundamental insights into glucose biosensor components and the crucial factors controlling the electrochemical performance of glucose biosensors are discussed in detail. The metal, metal oxide, and hybrid/composite nanomaterial fabrication strategies for the modification of electrodes, mechanism of detection, and significance of the nanomaterials toward the electrochemical performance of enzymatic and non-enzymatic glucose biosensors are compared and comprehensively reviewed. This review aims to provide readers with an overview and underlying concept of producing a reliable, stable, cost-effective, and excellent electrochemical performance of a glucose biosensor.
Microfluidic Liquid Cell with Silicon Nitride Super-Thin Membrane for Electron Microscopy of Samples in Liquid
Authors: Sugihara Akihiko , Ishida Tadashi
Published: December 2022

Microfluidic liquid cells have been developed to visualize nanoscaled biological samples in liquid using a scanning electron microscope (SEM) through an electron-transparent membrane (ETM). However, despite the combination of the high-resolution visualization of SEM and the high experimental capability of microfluidics, the image is unclear because of the scattering of the electron beam in the ETM. Thus, this study developed a microfluidic liquid cell with a super-thin ETM of thickness 10 nm. Because the super-thin ETM is excessively fragile, the bonding of a silicon–nitride-deposited substrate and a polydimethylsiloxane microchannel before silicon anisotropic etching was proposed prevented the super-thin ETM from damage and breakage due to etching. With this protection against etchant using the microchannel, the yield of the fabricated super-thin ETM increased from 0 to 87%. Further, the scattering of the electron beam was suppressed using a microfluidic liquid cell with a super-thin ETM, resulting in high-resolution visualization. In addition, T4 bacteriophages were visualized using a super-thin ETM in vacuum. Furthermore, the cyanobacterium Synechocystis sp. PCC6803 in liquid was visualized using a super-thin ETM, and sub-microscopic structures on the surface were observed.
Capture-SELEX: Selection Strategy, Aptamer Identification, and Biosensing Application
Authors: Lam Sin Yu , Lau Hill Lam , Kwok Chun Kit
Published: December 2022

Small-molecule contaminants, such as antibiotics, pesticides, and plasticizers, have emerged as one of the substances most detrimental to human health and the environment. Therefore, it is crucial to develop low-cost, user-friendly, and portable biosensors capable of rapidly detecting these contaminants. Antibodies have traditionally been used as biorecognition elements. However, aptamers have recently been applied as biorecognition elements in aptamer-based biosensors, also known as aptasensors. The systematic evolution of ligands by exponential enrichment (SELEX) is an in vitro technique used to generate aptamers that bind their targets with high affinity and specificity. Over the past decade, a modified SELEX method known as Capture-SELEX has been widely used to generate DNA or RNA aptamers that bind small molecules. In this review, we summarize the recent strategies used for Capture-SELEX, describe the methods commonly used for detecting and characterizing small-molecule–aptamer interactions, and discuss the development of aptamer-based biosensors for various applications. We also discuss the challenges of the Capture-SELEX platform and biosensor development and the possibilities for their future application.
Development and Evaluation of Automated Tools for Auditory-Brainstem and Middle-Auditory Evoked Potentials Waves Detection and Annotation
Authors: Manta Ourania , Sarafidis Michail , Vasileiou Nikolaos , Schlee Winfried , Consoulas Christos , Kikidis Dimitris , Vassou Evgenia , Matsopoulos George K. , Koutsouris Dimitrios D.
Published: December 2022

Auditory evoked potentials (AEPs) are brain-derived electrical signals, following an auditory stimulus, utilised to examine any obstructions along the brain neural-pathways and to diagnose hearing impairment. The clinical evaluation of AEPs is based on the measurements of the latencies and amplitudes of waves of interest; hence, their identification is a prerequisite for AEP analysis. This process has proven to be complex, as it requires relevant clinical experience, and the existing software for this purpose has little practical use. The aim of this study was the development of two automated annotation tools for ABR (auditory brainstem response)- and AMLR (auditory middle latency response)-tests. After the acquisition of 1046 raw waveforms, appropriate pre-processing and implementation of a four-stage development process were performed, to define the appropriate logical conditions and steps for each algorithm. The tools’ detection and annotation results, regarding the waves of interest, were then compared to the clinicians’ manual annotation, achieving match rates of at least 93.86%, 98.51%, and 91.51% respectively, for the three ABR-waves of interest, and 93.21%, 92.25%, 83.35%, and 79.27%, respectively, for the four AMLR-waves. The application of such tools in AEP analysis is expected to assist towards an easier interpretation of these signals.
Application of BRB to Seismic Mitigation of Steel Truss Arch Bridge Subjected to Near-Fault Ground Motions
Authors: Gao Haoyuan , Zhang Kun , Wu Xinyu , Liu Hongjiang , Zhang Lianzhen
Published: December 2022

In this paper, the seismic response of a steel truss arch bridge subjected to near-fault ground motions is studied. Then, the idea of applying buckling restrained braces (BRBs) to a steel truss arch bridge in near-fault areas is proposed and validated. Firstly, the basic characteristics of near-fault ground motions are identified and distinguished. Furthermore, the seismic response of a long span steel truss arch bridge in the near fault area is analyzed by elastic-plastic time analysis. Finally, the braces prone to buckling failure are replaced by BRBs to reduce the seismic response of the arch rib through their energy dissipation properties. Four BRB schemes were proposed with different yield strengths, but the same initial stiffness. The basic period of the structure remains the same. The results show that near-fault ground motion will not only obviously increase the displacement and internal force response of the bridge, but also cause more braces to buckle. By replacing a portion of the normal bars with BRBs, the internal forces and displacements of the arch ribs can be reduced to some extent, which is more prominent under the action of pulsed ground motion. There is a clear correlation between the damping effect and the parameters of BRB, so an optimized solution should be obtained by comparison and calculation.
Inverse Analysis of Straw Bale Mechanical Parameters in Load-Bearing Structures Based on a Finite Element Model
Authors: Lehner Petr , Brázdilová Hana
Published: December 2022

Scientific and practical research into alternative building materials is of high importance in terms of sustainability and ecology. Many variables have to be taken into account when using straw bales as load-bearing structures in residential buildings. The main problems are the lack of information on the mechanical properties of this material and its potential high variability. The development of numerical FEM models based on accurate experiments can help to better understand the behaviour of this material. The main objective of this paper is to present a simplified isotropic model of straw bales based on measured data from a laboratory experiment, which will facilitate the preparation and evaluation of further future experiments. Already partially published data of compression tests of load-bearing straw bales were analysed. Using an automated algorithm, an estimate of the elastic modulus of the bale was determined, and inverse analyses were performed using accurate FEM numerical models based on similarity to the force-deformation diagram. In all experiments, it was found that the ideal combination is elastic modulus at 20% load and Poisson’s constant of 0.2. From the results, further experimental directions can be determined, mainly considering a larger number of specimens with different properties. These and other findings provide the basis for the ever-expanding field of research on load-bearing straw bales in construction.
Direct Z-Scheme Heterojunction α-MnO2/BiOI with Oxygen-Rich Vacancies Enhanced Photoelectrocatalytic Degradation of Organic Pollutants under Visible Light
Authors: Jia Litao , Li Fanghua , Yang Chenjia , Yang Xiaonan , Kou Beibei , Xing Yonglei , Peng Juan , Ni Gang , Cao Zhong , Zhang Shiyu , Zhao Tong , Jin Xiaoyong
Published: December 2022

The degradation efficiency of photoelectrocatalytic (PEC) processes for the removal of organic pollutants is highly dependent on the performance of the photoelectroanode catalyst. The design of PEC systems with a direct Z-scheme charge transfer mechanism and visible light excitation is essential to enhance the degradation efficiency of organic compounds. Here, a α-MnO2/BiOI direct Z-scheme heterojunction photocatalyst was successfully synthesized through a convenient and feasible method. It is remarkable that the photoanode exhibited excellent PEC performance under visible light irradiation; a 95% removal rate of tetracycline (TC) pollutants was achieved within 2 h, and it had excellent stability and reusability, which was expected to degrade antibiotics efficiently and environmentally in harsh environments. The presence of oxygen vacancies (OVs) in the α-MnO2/BiOI heterojunction was confirmed by electron spin resonance technique, and the OVs acted as electron traps that contributed substantially to the separation efficiency of photogenerated carriers. ESR characterization showed that the main reactive radicals during TC degradation were •OH and •O2−. By analyzing the intermediates, the possible degradation pathways of TC were further analyzed and a suitable degradation mechanism was proposed. The toxicity changes in the degradation process were explored by evaluating the toxicity of the intermediates. This study provides a new way to enhance the performance of Bi-based semiconductor photocatalysts for the effective degradation of TC in water.
Evaluation of Cardiovascular Toxicity of Folic Acid and 6S-5-Methyltetrahydrofolate-Calcium in Early Embryonic Development
Authors: Lian Zenglin , Wu Zhuanbin , Gu Rui , Wang Yurong , Wu Chenhua , Cheng Zhengpei , He Mingfang , Wang Yanli , Cheng Yongzhi , Gu Harvest F.
Published: December 2022

Folic acid (FA) is a synthetic and highly stable version of folate, while 6S-5-methyltetrahydrofolate is the predominant form of dietary folate in circulation and is used as a crystalline form of calcium salt (MTHF-Ca). The current study aims to evaluate the toxicity and safety of FA and MTHF-Ca on embryonic development, with a focus on cardiovascular defects. We began to analyze the toxicity of FA and MTHF-Ca in zebrafish from four to seventy-two hours postfertilization and assessed the efficacy of FA and MTHF-Ca in a zebrafish angiogenesis model. We then analyzed the differently expressed genes in in vitro fertilized murine blastocysts cultured with FA and MTHF-Ca. By using gene-expression profiling, we identified a novel gene in mice that encodes an essential eukaryotic translation initiation factor (Eif1ad7). We further applied the morpholino-mediated gene-knockdown approach to explore whether the FA inhibition of this gene (eif1axb in zebrafish) caused cardiac development disorders, which we confirmed with qRT-PCR. We found that FA, but not MTHF-Ca, could inhibit angiogenesis in zebrafish and result in abnormal cardiovascular development, leading to embryonic death owing to the downregulation of eif1axb. MTHF-Ca, however, had no such cardiotoxicity, unlike FA. The current study thereby provides experimental evidence that FA, rather than MTHF-Ca, has cardiovascular toxicity in early embryonic development and suggests that excessive supplementation of FA in perinatal women may be related to the potential risk of cardiovascular disorders, such as congenital heart disease.
Apple Derived Exosomes Improve Collagen Type I Production and Decrease MMPs during Aging of the Skin through Downregulation of the NF-κB Pathway as Mode of Action
Authors: Trentini Martina , Zanolla Ilaria , Zanotti Federica , Tiengo Elena , Licastro Danilo , Dal Monego Simeone , Lovatti Luca , Zavan Barbara
Published: December 2022

Skin ageing is strictly related to chronic inflammation of the derma and the decay of structural proteins of the extracellular matrix. Indeed, it has become common practice to refer to this phenomenon as inflammageing. Biotech innovation is always in search of new active principles that induce a youthful appearance. In this paper, apple-derived nanovesicles (ADNVs) were investigated as novel anti-inflammatory compounds, which are able to alter the extracellular matrix production of dermal fibroblasts. Total RNA sequencing analysis revealed that ADNVs negatively influence the activity of Toll-like Receptor 4 (TLR4), and, thus, downregulate the NF-κB pro-inflammatory pathway. ADNVs also reduce extracellular matrix degradation by increasing collagen synthesis (COL3A1, COL1A2, COL8A1 and COL6A1) and downregulating metalloproteinase production (MMP1, MMP8 and MMP9). Topical applications for skin regeneration were evaluated by the association of ADNVs with hyaluronic-acid-based hydrogel and patches.
Transcriptome Analysis Unveils That Exosomes Derived from M1-Polarized Microglia Induce Ferroptosis of Neuronal Cells
Authors: Gao Sheng , Jia Shu , Bai Luyue , Li Dongru , Meng Chunyang
Published: December 2022

Microglia play a vital role in neurodegenerative diseases. However, the effects of microglia-derived exosomes on neuronal cells are poorly understood. This study aimed to explore the role of M1-polarized microglia exosomes in neuronal cells by transcriptome analysis. Exosomes isolated from resting M0-phenotype BV2 (M0-BV2) microglia and M1-polarized BV2 (M1-BV2) microglia were analyzed using high-throughput sequencing of the transcriptome. Differentially expressed genes (DEGs) between the two types of exosomes were identified by analyzing the sequencing data. The biological functions and pathways regulated by the identified DEGs were then identified using bioinformatics analyses. Finally, we evaluated the effects of exosomes on neuronal cells by coculturing M0-BV2 and M1-BV2 exosomes with primary neuronal cells. Enrichment analyses revealed that DEGs were significantly enriched in the ferroptosis pathway (p = 0.0137). M0-BV2 exosomes had no distinct effects on ferroptosis in neuronal cells, whereas M1-BV2 exosomes significantly reduced ferroptosis suppressor proteins (GPX4, SLC7A11, and FTH1) and elevated the levels of intracellular and mitochondrial ferrous iron and lipid peroxidation in neuronal cells. Polarized M1-BV2 microglia exosomes can induce ferroptosis in neuronal cells, thereby aggravating neuronal damage. Taken together, these findings enhance knowledge of the pathogenesis of neurological disorders and suggest potential therapeutic targets against neurodegenerative diseases.
Two-Step Conversion of CO2 to Light Olefins: Laboratory-Scale Demonstration and Scale-Up Considerations
Authors: Reinikainen Matti , Braunschweiler Aki , Korpilo Sampsa , Simell Pekka , Alopaeus Ville
Published: December 2022

The highly selective production of light olefins from CO2 was demonstrated for the first time with a laboratory-scale process comprising consecutive reverse water gas shift (RWGS) and Fischer–Tropsch (FT) reactors. The RWGS reaction, catalyzed by rhodium washcoated catalyst at 850 °C yielded good quality syngas with conversion values close to the thermodynamic equilibrium and without experiencing catalyst deactivation from carbon formation or sintering. For the FT synthesis, a packed bed Fe-Na-S/α-Al2O3 catalyst was used. The highest light olefin selectivity observed for the FT-synthesis was 52% at 310 °C, GHSV of 2250 h−1 and H2/CO ratio of 1. However, the optimal conditions for the light olefin production were determined to be at 340 °C, a GHSV of 3400 h−1 and a H2/CO ratio of 2, as the CO conversion was markedly higher, while the light olefin selectivity remained at a suitably high level. In addition to the experimental results, considerations for the further optimization and development of the system are presented. The combined RWGS–FT process seems to work reasonably well, and initial data for basic process design and modeling were produced.
Facile Solvent-Free Mechanochemical Synthesis of UI3 and Lanthanoid Iodides
Authors: Werner Daniel , Badea Désirée , Schönzart Jasmin , Eimermacher Sophia , Bätz Philipp , Wickleder Mathias S. , Zegke Markus
Published: December 2022

Lewis base-free lanthanoid (Ln) and actinoid (An) iodides are difficult to obtain, as standard protocols describe syntheses in solutions of donor solvents which are ultimately hard to remove. We have now established a mechanochemical approach towards the synthesis of Lewis base-free f-block metal iodides with excellent yields. In particular, we describe herein the synthesis of EuI2 as an example of a divalent lanthanoid iodide, of CeI3 as an example of a trivalent lanthanoid iodide, and of UI3 as the most important actinoid iodide. Each can be obtained in high yield with minimal work-up, presenting the most efficient and simple synthetic route to access these materials to date.
Study on Nutritional Knowledge, Attitude and Behavior of Chinese School Football Players
Authors: Chen Yao , Sun Yingshuang , Liu Zhiyun , Hu Donglin
Published: December 2022

Objective: This study aims to validate previous structural models of factors influencing dietary behavior changes and construct the knowledge, attitude, and behavioral models of youth school football players. Methods: 279 school football players aged 12–17 years in Grades 7–12 in Hunan Province, China, completed a questionnaire to collect data on sports nutrition knowledge, attitudes, and behavior. A structural equation model (SEM) was built based on the knowledge-attitude-behavior (KAB) model and the theory of planned behavior (TPB) model to introduce nutritional knowledge directly or by altering attitudes into the dietary behavior path of players. Four factors affecting dietary behaviors were involved in the hypothetical structure, which consists of the following hypotheses: (1) nutrition knowledge affects the attitude towards sports nutrition (H1); (2) nutrition knowledge affects dietary behaviors (H2); (3) nutrition knowledge affects subjective norms (H3); (4) nutrition knowledge affects perceived behavioral control (H4); (5) subjective norms affect dietary behaviors (H5); and 6) perceived behavioral control affects dietary behaviors (H6). Results: Confirmatory factor analysis (CFA) revealed that the reliability, convergent validity, and discriminant validity of the built SEM conformed to the measured relationships in each dimension. In the final structural model, it was found that nutrition knowledge had a direct impact on the attitudes of players and affected their dietary behaviors in a direct manner or through their subjective norms and perceived behavioral control. Conclusions: The results are in agreement with the TPB-based KAB chain and support the KAB theory for youth school football players in Hunan Province, China.
The Impacts of Armed Conflicts and Civilian Uprisings on Children’s Health
Authors: Khorram-Manesh Amir
Published: December 2022

Besides the most common causes of death, children under 14 may suffer from the consequences of disasters and emergencies caused by natural and manmade risks and hazards. These incidents may be prevented by proper risk assessment and the prevention of unnecessary deaths by increasing the safety and well-being of children. An increasing number of manmade incidents are seen in international, non-international, and domestic conflicts when government forces use heavy and light weapons within countries with autocratic leadership. These deaths should be mitigated by holding perpetrators responsible for their deeds and respecting international humanitarian law, human rights, and children’s rights.
Effects of Four Weeks of Plyometric Training Performed in Different Training Surfaces on Physical Performances in School Children: Age and Sex Comparisons
Authors: Marzouki Hamza , Ouergui Ibrahim , Dridi Rached , Selmi Okba , Mbarki Rania , Mjadri Nour , Thuany Mabliny , Andrade Marilia S. , Bouhlel Ezdine , Weiss Katja , Knechtle Beat
Published: December 2022

Short- to middle-term plyometric training has been shown to be an effective method to promote youth fitness and health. However, there is no knowledge of previous studies that investigated the sex and age effects on physical fitness following different PT surfaces (i.e., firm vs. sand) in schoolchildren. This study examined the effects of age and sex on explosive and high-intensity responses following plyometric training (4 weeks, twice/week) performed on firm vs. sand surfaces in untrained schoolchildren. Ninety girls and ninety boys (under 8: age = 7.1 ± 0.5 and 7.1 ± 0.4 years; under 10: age = 9.0 ± 0.4 and 9.0 ± 0.5 years; under 12: age = 11.0 ± 0.5 and 11.0 ± 0.5 years, respectively) participated in a randomized and parallel training design with pre-to-post testing. Participants were allocated (i.e., 30 boys and 30 girls for each group) into either two experimental groups (firm group: performing plyometrics on a clay surface and sand group: performing plyometrics on a dry surface of 20 cm deep sand) or a control group (CG, habitual physical education classes) within their corresponding age groups. Children were tested for sprint, jumping and change of direction speed performances before and after 4 weeks of plyometric training. Both experimental groups induced more significant improvements in all assessed variables than CG (p < 0.0001; effect size > 0.80), whereas both surfaces induced similar improvements (p > 0.05). Older boys achieved better performances than their younger counterparts (p < 0.05) and older girls (p < 0.0001), respectively. This finding showed that age and sex could affect explosive and high-intensity performances during childhood after a short-term plyometric training. In contrast, the training-induced fitness changes were not influenced by the type of surface.
Structural Characteristics and Sound Absorption Properties of Waste Hemp Fiber
Authors: Zhang Duoduo , Zhou Xinghai , Gao Yuan , Lyu Lihua
Published: December 2022

In order to realize high-efficiency and high-value recycling of waste hemp fibers, the macromolecular structure, the supramolecular structure, and the morphological structure of waste hemp fibers were investigated by using Fourier transform infrared spectroscopy, X-ray diffractometry, upright metallurgical microscopy, and scanning electron microscopy. According to its structural characteristics, the sound-absorbing mechanism of waste hemp fiber was analyzed, and the reason for the good sound-absorbing performance of waste hemp fiber was clarified. The acoustic impedance transfer function test was used to analyze and compare the sound-absorbing performance of waste hemp fiber and several other fiber aggregates that could be used in the field of sound-absorbing, and the sound-absorbing performance of a waste hemp fiber composite material was tested. The research revealed that: the sequence of sound-absorbing performance of several fiber aggregates was cotton fiber, waste hemp fiber, wool fiber, and polyester fiber; that waste hemp fiber had excellent high-frequency sound-absorbing performance, with a maximum sound absorption coefficient of 0.95; and that the maximum sound absorption coefficient of the waste hemp fiber composite reached 0.93. Therefore, the waste hemp fiber has excellent sound-absorbing properties and has high application value in the field of sound absorption.
Investigation of Roughness and Adhesion Strength Properties of Pine and Poplar Wood Heat Treated in Air and under Vacuum after Artificial Aging
Authors: Krystofiak Tomasz , Can Ahmet , Lis Barbara
Published: December 2022

Heat treatment is an eco-friendly and efficient way to improve the defective properties of wood, such as its hygroscopic nature, the lack of dimensional stability, and low resistance against biological degradation, and to produce a green and sustainable wood material for construction and buildings. However, these treatments alter the substrates and could influence the performance of the coating products necessary to maintain the surface features in certain end-use sectors. In this study, the effects of heat treatment in air (HT) and under vacuum (VHT) on the surface properties of Scots pine (Pinus sylvestris L.) and poplar (Populus euramericana) wood were investigated. For this purpose, the samples were exposed to an artificial aging process. After the aging process, surface roughness and the adhesion strength behavior of the samples were measured. After the HT and VHT, poplar and pine wood samples showed different roughness. While the roughness value parallel to the fibers decreased in poplar wood, it increased in pine wood. The roughness value perpendicular to the fibers increased in both tree species. The maximum roughness value after UV aging was 62,622 in the VHT-200 group. Although with the UV ageing treatment, the adhesion strength was decreased in the samples heat treated in air, it was increased in the samples subjected to vacuum heat treatment. A lower loss of adhesion strength was observed in the heat-treated samples processed under vacuum compared to the heat-treated samples processed in air.