bluuebunny/crossref_metadata_2025 huggingface dataset:
Ferroptosis in Hepatocellular Carcinoma: Mechanisms, Drug Targets and Approaches to Clinical Translation
Authors: Bekric Dino , Ocker Matthias , Mayr Christian , Stintzing Sebastian , Ritter Markus , Kiesslich Tobias , Neureiter Daniel
Published: April 2022

Ferroptosis, an iron and reactive oxygen species (ROS)-dependent non-apoptotic type of regulated cell death, is characterized by a massive iron overload and peroxidation of polyunsaturated fatty acids (PUFAs), which finally results in cell death. Recent studies suggest that ferroptosis can influence carcinogenesis negatively and therefore may be used as a novel anti-cancer strategy. Hepatocellular carcinoma (HCC) is a deadly malignancy with poor chances of survival and is the second leading cause of cancer deaths worldwide. Diagnosis at an already late stage and general resistance to current therapies may be responsible for the dismal outcome. As the liver acts as a key factor in iron metabolism, ferroptosis is shown to play an important role in HCC carcinogenesis and, more importantly, may hold the potential to eradicate HCC. In this review, we summarize the current knowledge we have of the role of ferroptosis in HCC and the application of ferroptosis as a therapy option and provide an overview of the potential translation of ferroptosis in the clinical practice of HCC.
Drosophila as a Model of Unconventional Translation in Spinocerebellar Ataxia Type 3
Authors: Johnson Sean L. , Prifti Matthew V. , Sujkowski Alyson , Libohova Kozeta , Blount Jessica R. , Hong Luke , Tsou Wei-Ling , Todi Sokol V.
Published: April 2022

RNA toxicity contributes to diseases caused by anomalous nucleotide repeat expansions. Recent work demonstrated RNA-based toxicity from repeat-associated, non-AUG-initiated translation (RAN translation). RAN translation occurs around long nucleotide repeats that form hairpin loops, allowing for translation initiation in the absence of a start codon that results in potentially toxic, poly-amino acid repeat-containing proteins. Discovered in Spinocerebellar Ataxia Type (SCA) 8, RAN translation has been documented in several repeat-expansion diseases, including in the CAG repeat-dependent polyglutamine (polyQ) disorders. The ATXN3 gene, which causes SCA3, also known as Machado–Joseph Disease (MJD), contains a CAG repeat that is expanded in disease. ATXN3 mRNA possesses features linked to RAN translation. In this paper, we examined the potential contribution of RAN translation to SCA3/MJD in Drosophila by using isogenic lines that contain homomeric or interrupted CAG repeats. We did not observe unconventional translation in fly neurons or glia. However, our investigations indicate differential toxicity from ATXN3 protein-encoding mRNA that contains pure versus interrupted CAG repeats. Additional work suggests that this difference may be due in part to toxicity from homomeric CAG mRNA. We conclude that Drosophila is not suitable to model RAN translation for SCA3/MJD, but offers clues into the potential pathogenesis stemming from CAG repeat-containing mRNA in this disorder.
Ecological Links between Pelagic and Mesophotic Reef Fishes in an Oceanic Archipelago of the Equatorial Atlantic Ocean
Authors: Pimentel Caio Ribeiro , Pinheiro Hudson T. , Giarrizzo Tommaso , Francini-Filho Ronaldo B. , Reis-Filho José Amorim , Rocha Luiz A. , Ferreira Carlos Eduardo L. , Barbosa Moysés Cavichioli , Joyeux Jean-Christophe
Published: April 2022

Geographic isolation, a relatively low species richness and high endemism make oceanic islands excellent natural laboratories for ecological and evolutionary studies. Here we used Baited Remote Underwater stereo-Video systems (stereo-BRUVS) to investigate the taxonomic, trophic and size structures of fish assemblages from pelagic and mesophotic reef ecosystems in the smallest archipelago of the equatorial Atlantic Ocean, the Saint Peter and Saint Paul’s Archipelago (SPSPA). The occurrence of steep reef walls favors studies on the ecological connections between pelagic and reef ecosystems. We performed five pelagic stereo-BRUVS deployments and fourteen benthic stereo-BRUVS deployments, totaling 1440 min of footage. We recorded 14 species from eight families in the pelagic ecosystem, with Carangidae and Balistidae as the most diverse families. The most abundant species were Elagatis bipinnulata (Quoy and Gaimard, 1825), Melichthys niger (Bloch, 1786), and Caranx crysos (Mitchill, 1815), which together accounted for over 75% of the total relative abundance. The carnivores were the most diverse and abundant trophic group. On the mesophotic reefs, 41 taxa were recorded, with carnivores and planktivores being the most diverse, whereas omnivores were the most abundant. Here, the most abundant species were M. niger, Azurina multileneata, Chromis vanbebberae, Seriola rivoliana, Caranx lugubris and Stegastes sanctipauli. Nine species were recorded in both ecosystems, with Melichthys niger, Caranx lugubris and S. rivoliana being the main species linking them (i.e., occurring in both). These species are known to forage in both pelagic and mesophotic reef ecosystems, and thus represent potential ecological links between them. Such links combined with the endangered status of some species, suggest the need for an integrated management strategy in this remote archipelago.
Promising Genomic Testing for Biliary Tract Cancer Using Endoscopic Ultrasound-Guided Fine-Needle Aspiration/Biopsy Specimens
Authors: Kuwatani Masaki , Kawakubo Kazumichi , Sakamoto Naoya
Published: April 2022

The undesired prognosis of biliary tract cancer is mainly attributed to the difficult detection of cancer lesions, including intraepithelial neoplasia and no standard examination for screening. In addition, pathological diagnosis of biliary stricture, whether it is malignant or benign, is not so easy, because of difficult optimal sampling by forceps biopsy and brush cytology, although various devices and methods for pathological diagnosis have been reported. Furthermore, we have to be careful about post-endoscopic retrograde cholangiography pancreatitis when we approach the biliary tract lesion via a transpapillary route. In order to improve the diagnostic accuracy, there have been several studies that indicate the feasibility and efficacy of genomic analysis for accurate diagnosis of biliary tract cancer by using pathological specimens, including endoscopic ultrasound-guided fine-needle aspiration/biopsy (EUS-FNA/FNB) samples. For efficient and precision medicine for patients with biliary tract cancer, future diagnosis and treatment should also be based on molecular and genetic analyses. In this article, we review and summarize the past knowledge and cutting edge of genomic testing for biliary tract cancer, using EUS-FNA/FNB specimens, and indicate some ingenuities in sample processing to promote effective clinical practice and future perspectives.
OntoDomus: A Semantic Model for Ambient Assisted Living System Based on Smart Homes
Authors: Ngankam Hubert Kenfack , Pigot Hélène , Giroux Sylvain
Published: April 2022

Ambient assisted living (AAL) makes it possible to build assistance for older adults according to the person’s context. Understanding the person’s context sometimes involves transforming one’s home into a smart home. Typically, this is carried out using nonintrusively distributed sensors and calm technologies. Older adults often have difficulty performing activities of daily living, such as taking medication, drinking coffee, watching television, using certain electronic devices, and dressing. This difficulty is even greater when these older adults suffer from cognitive impairments. Defining an assistance solution requires a multidisciplinary and iterative collaborative approach. It is necessary, therefore, to reason about the imperatives and solutions of this multidisciplinary collaboration (e.g., clinical), as well as the adaptation of technical constraints (e.g., technologies). A common approach to reasoning is to represent knowledge using logic-based formalisms, such as ontologies. However, there is not yet an established ontology that defines concepts such as multidisciplinary collaboration in successive stages of the assistance process. This article presents OntoDomus, an ontology that describes, at several levels, the semantic interactions between ambient assisted living, context awareness, smart home, and Internet of Things, based on multidisciplinarity. It revolves around two main notions: multidisciplinarity, based on specific sub-ontologies and the ambient feedback loop. OntoDomus combines SPARQL queries and OWL 2 models to improve the reusability of domain terminology, allowing stakeholders to represent their knowledge in different collaborative and adaptive situations. The ontological model is validated, first by its reuse in more specific works—specific to an aspect of ambient assistance. Second, it is validated by the structuring of ambient knowledge and inferences of the formalization in a case study that includes instances for a particular activity of daily living. It places the ambient feedback loop at the center of the ontology by focusing on highly expressive domain ontology formalisms with a low level of expressiveness between them.
Methods to Improve Dynamic System Response of Power Compensators Using Supercapacitors in Low-Voltage Ride-Through (LVRT) Conditions
Authors: Kim Mi-na , Yi Jun-sin , Won Chung-Yuen , Lee Jung-Hyo
Published: April 2022

In this paper, a power compensator using supercapacitors in parallel to protect grid-connected devices connected to the distributed power supply in the case of a low-voltage ride-through (LVRT) situation in designed, and a grid-connected device control method with improved responsiveness is proposed. In the LVRT situation, the distributed generation power may boost the DC_link voltage, increasing the risk of destroying grid-connected devices. To prevent this, the power compensator designed in this study absorbs active power in a fault situation and stores it in the supercapacitor to suppress the DC_link voltage rise and efficiently use the power. In addition, we propose methods to improve the response of the grid reactive power through the reactive power compensation of the power compensator in LVRT situation. To this end, the power angle (θPW) was extracted through the formula, and the reactive power command, to be compensated by the power compensator, and the reactive power command, compensated by the grid-connected devices, were calculated according to the active power value. In this way, the grid power controlled by the power compensation device and the grid-connected devices was controlled by the active/reactive power of the same power angle and analyzed mathematically. Active power control and static grid support were performed in the normal state where the reduction rate of the normal value of the grid voltage was around 10%. However, when the grid voltage dropped by 10% to 100%, the reactive power control was appropriately performed with dynamic grid support by increasing the voltage from 10% to 20% or more. We conducted a simulation of the new and renewable energy grid-connected devices using the OPAL-RT-based Hardware-in-the Loop Simulation (HILS) system to control the proposed active/reactive power.
An Electro-Thermal Model for LFP Cells: Calibration Procedure and Validation
Authors: Barbieri Michele , Ceraolo Massimo , Lutzemberger Giovanni , Scarpelli Claudio
Published: April 2022

Lithium batteries for energy storage systems are a prominent solution for both stationary and mobile applications. Electro-thermal modelling of the cell is a useful tool for monitoring voltage and temperature in order to predict battery behaviour especially in cases of critical operative conditions. This paper provides a modelling approach focusing on the calibration of parameters of an electro-thermal model for large prismatic LFP lithium cells. The designed model is tuned by means of experimental tests that identify a set of parameters that are function of a cell’s state-of-charge and temperature. The model outputs are voltage, cell surface, and internal temperature profiles, which are validated against experimental data referring to realistic working conditions, even providing an intense level of thermal stress. The model accuracy is marked by a voltage mean average error lower than 1% and a mean cell surface temperature deviation lower than 1 K.
Assessment of Environmental Pollution in Cement Plant Areas in Romania by Co-Processing Waste in Clinker Kilns
Authors: Ciobanu Cristian , Tudor Paula , Istrate Irina-Aura , Voicu Gheorghe
Published: April 2022

Worldwide, in the rotary kilns of cement plants, various amounts of combustible waste are burned, which would otherwise end up in municipal landfills. The paper first analyzes the literature on the co-processing of waste in cement manufacture and its influence on the environment and human health. Then, it shows how the combustion components of co-processed waste can influence the final characteristics of clinker and cement. The main objective of the paper is to determine the level of emissions of the resulting pollutants (total dust and flue gases: NOx, SO2, and CO) at the outlet of the chimney of the clinker kiln and flour mill to meet the requirements of the Integrated Environmental Permit (EIA) from Romania and present an estimation of the level of atmospheric air pollution using the climatological model of pollutant dispersion. Following these assessments (data shown), the cement factories will establish measures to reduce the pollution, if necessary, to comply with the regulations in force for the cement industry.
CO2 Emission Factors and Carbon Losses for Off-Road Mining Trucks
Authors: de Carvalho João Andrade , de Castro André , Brasil Gutemberg Hespanha , de Souza Paulo Antonio , Mendiburu Andrés Z.
Published: April 2022

There are myriad questions that remain to be answered in greenhouse gas (GHG) emissions trading. This article addresses carbon dioxide (CO2) emission factors and carbon losses from heavy equipment that is used to transport ores. Differences occurred between the Intergovernmental Panel for Climate Change (IPCC) emission factor and those that were obtained by considering incomplete combustion and on-site exhaust concentration measurements. Emissions from four off-road vehicles were analyzed. They operated at idle (loading, unloading, and queuing) and in motion (front and rear, loaded and unloaded). The results show that the average CO2 emission factors can be as low as 64.8% of the IPCC standard value for diesel fuel. On the other hand, carbon losses can be up to 33.5% and energy losses up to 25.5%. To the best of the authors’ knowledge, the method that was developed here is innovative, simple, useful, and easily applicable in determining CO2 emission factors and fuel losses for heavy machinery.
Development of a Climate-Sensitive Matrix Growth Model for Larix gmelinii Mixed-Species Natural Forests and Its Application for Predicting Forest Dynamics under Different Climate Scenarios
Authors: Zhang Liang , He Youjun , Wang Jianjun , Meng Jinghui
Published: April 2022

Larix gmelinii natural forests, which are of great ecological and economic importance, are mainly distributed in northeast China. Sustainable management of these forests play a vital role in ecological security in northeast China, especially in the context of climate change. Forest growth models, which support forest management decision-making, are lacking for Larix gmelinii natural forests, hampering the prescription of forest management strategies. In this study, we produced a climate-sensitive, transition-matrix model (CM) for Larix gmelinii natural forests. For comparison, a variable transition model without including climate change effects (NCM) and a fixed-parameter model (FM) were also built. We examined the performance of the CM, NCM, and FM by conducting short- (5 years) and long-term (100 years) simulations. The results showed that for short-term prediction, no significant difference was observed among the three predictive models. However, the long-term prediction ability of the CM under the three different RCPs was superior to that of the FM and NCM. The number of trees and basal area were predicted to increase under climate change, which might result in natural disasters, such as snow break, windthrow, and forest fire. Silvicultural practices, such as reducing the intermediate thinning interval and the enrichment planting of slow-growing trees, should be implemented to mitigate the deleterious effects of climate change.
Do We Have a New Song Yet? The New Wave of Women’s Novels and the Homeric Tradition
Authors: Goff Barbara
Published: April 2022

The relationship between women and classical antiquity, its texts, artefacts, and study, has been fraught to say the least; the discipline of Classics has often been defined by the exclusion of women, in terms of their education and their ability to contribute to debates more generally. However, we are currently in the middle of an astonishing period when women are laying more of a claim to the discipline than ever before. This article examines three recent novels by women which take on the cultural weight of the Homeric epics, Iliad and Odyssey, to explore the possibilities of a ‘new song’ that foregrounds female characters. The novels experiment with different narrative voices and are self-conscious about the practices of story-telling and of bardic song. Their awareness of their challenge to and contest with Homeric tradition renders their ‘new songs’ fragile as well as precious.
Stress in Emergency Healthcare Professionals: The Stress Factors and Manifestations Scale
Authors: García-Tudela Ángel , Simonelli-Muñoz Agustín Javier , Rivera-Caravaca José Miguel , Fortea María Isabel , Simón-Sánchez Lucas , González-Moro María Teresa Rodríguez , González-Moro José Miguel Rodríguez , Jiménez-Rodríguez Diana , Gallego-Gómez Juana Inés
Published: April 2022

Background: Healthcare workers are continuously exposed to a high level of stress, especially emergency department professionals. In the present research, we aimed to determine the internal consistency and validity of the Stress Factors and Manifestations Scale for in-hospital and out-of-hospital emergency workers. Methods: A quantitative, prospective, cross-sectional, and observational study including 269 emergency service professionals. Results: The scale was composed of 21 items, with a Cronbach’s α value of 0.908. The hospital workers (38.4 ± 10.8 vs. 35.1 ± 9.9, p = 0.014) and women (39.3 ± 11.4 vs. 34.2 ± 8.6, p < 0.001) had higher levels of stress. The sensitivity, specificity, and predictive values of the scale were adequate. Conclusion: In the present study, including in-hospital and out-of-hospital emergency workers, the Stress Factors and Manifestations Scale presented appropriate usefulness, internal consistency, and validity, with optimal predictive ability. Higher levels of anxiety, female gender, being less optimistic, and working in hospital emergency departments were related to increased stress levels. Further studies are warranted to validate our results and potentially extend the Stress Factors and Manifestations Scale to other contexts.
The Effect of Denture Cleansing Solutions on the Retention of Precision Attachments: An In Vitro Study
Authors: Deste Gokay Gonca , Ozkir Serhat Emre , Wolf Thomas Gerhard , Gokcimen Gulsum , Rona Nergiz , Bicer Mehmet , Yilmaz Burak
Published: April 2022

This study aimed to investigate the effect of different cleansing solutions on the retention of precision attachments. A precision attachment patrix was embedded into acrylic resin and the matrix was placed onto the patrix. The red (high retention, 8 N), yellow (regular retention, 6 N), and green (reduced retention, 4 N) plastic matrixes of the attachments (n = 32) were soaked in three different denture cleansing solutions (sodium laureth sulfate, sodium bicarbonate-sodium perborate, sodium bicarbonate) for a duration simulating 6 months of clinical use. The control group was soaked in tap water. A universal testing machine was used to measure the retention values of attachments after they were soaked in denture cleansers. The retention values were compared among the groups with repeated-measures analysis of variance followed by the Tukey HSD test (p = 0.05). Yellow attachments were affected by sodium laureth sulfate, sodium bicarbonate-sodium perborate, and water (p = 0.012). Green attachments’ retention increased after immersion in sodium laureth sulfate (p = 0.04) and water (p = 0.02). Red attachments’ retention increased after immersion in sodium laureth sulfate or sodium bicarbonate-sodium perborate (p = 0.045). Water did not affect the retention of red attachments. Because sodium bicarbonate tablets did not affect the retention of attachments, clinicians may recommend their use as a cleanser. Clinicians also may inform patients using fixed and removable partial prostheses with precision attachments of a possible increase in retention after the use of sodium laureth sulfate or when using sodium bicarbonate-sodium perborate with yellow and red attachments.
What We Know and What Remains to Be Explored about LGBTQ Parent Families in Israel: A Sociocultural Perspective
Authors: Shenkman Geva , Segal-Engelchin Dorit , Taubman–Ben-Ari Orit
Published: April 2022

This paper reviews research on gay and lesbian parent families in Israel through cultural lenses while recognizing the diversity of these families. The major aims of the review are: (1) to provide an overview of the situation of LGBTQ parent families in Israel, as well as of the sociocultural background of the Israeli context and its effects on sexual minorities and LGBTQ parent families; and (2) to identify the limitations and lacunas in the existing research and shed light on what remains to be explored. We searched numerous databases for relevant studies, adopting a narrative approach to summarize the main findings while taking into account the literature on the socio-cultural context in Israel and its impact on sexual minorities and LGBTQ parent families. The search yielded empirical results only for gay and lesbian parent families, with studies emphasizing the challenges they face and the factors related to their well-being and that of LGB individuals aspiring to become parents. In addition, it revealed that research on children’s psychosocial adjustment as a function of parental sexual orientation is quite scarce in Israel. Moreover, it indicated the absence of investigations of bisexual, transgender, or queer parents. We conclude that the sociocultural context of Israel, including its pronatalist and familistic orientation, may play an important role in shaping the experiences of LGBTQ parent families, and should be taken into consideration when studying LGBTQ parents.
Evolution of Scientific Production on Health Literacy and Health Education—A Bibliometric Analysis
Authors: Selva-Pareja Laia , Ramos-Pla Anabel , Mercadé-Melé Pere , Espart Anna
Published: April 2022

In the last few years, there has been an emphasis on the importance of health literacy (HL) and health education (HE) as basic tools to empower individuals and the community. The increasing interest in HL and HE has been observed through the evolution of publications and the nature of the main trends in the last few years. Knowing how HL and HE have evolved in scientific publications can help us to identify trends and set work priorities in this scope. Based on this, a bibliometric analysis (from 2000 to 2021) was conducted in two phases: first, an analysis was performed on the publications included in the Web of Science (WOS); second, a more specific analysis was conducted on the Core Collection from WOS. The data were analyzed with two software programs, the and Bibliometrix package for RStudio, and VOSviewer to analyze number of publications, citations, authors, collaborations, keywords trends, keywords evolutions and clusters of related terms. A total of 1799 articles were found in the first phase, and 727 in the second. The results from both analyses showed that the publications increased unequally until 2020, and considerably decreased in 2021; however, in spite of this, the number of citations remained constant. Likewise, five word clusters related with HL and HE were identified. D. Nutbeam stood out as the most prolific author on the subject, the USA as the country with the most publications, and the International Journal of Environmental Research and Public Health as having the most articles on the subject. This analysis may be a useful and helpful tool for future studies on the subject.
The Association between Nursing Skill Mix and Patient Outcomes in a Mental Health Setting: Protocol for an Observational Feasibility Study
Authors: Moyo Nompilo , Jones Martin , Dennis Shaun , Sharma Karan , Gray Richard
Published: April 2022

International research on nursing skill mix has focused primarily on medical and surgical patient populations. The association between nursing skill mix and clinical outcomes for psychiatric inpatients has not been explored. The aim of this study is to establish the feasibility of extracting and linking nurse and inpatient data from hospital administrative data sources. This is an observational study. Data will be extracted from hospital administrative sources and linked together. Patient information will include duration and number of psychiatric hospital admissions. We will extract information on the educational preparation of nurses working in the participating hospitals to enable us to calculate estimates of the nursing skill mix. The study will be conducted in two psychiatric inpatient services in Australia. Our study will test the feasibility of extracting and linking nursing skill mix and patient data in a mental health setting and will inform the methodological development of an appropriately powered observational study. Australian and New Zealand clinical trial registry: ACTRN12619001337167p.
Adaptation and Validation of the Lithuanian-Language Version of the Olympic Value Scale (OVS)
Authors: Sukys Saulius , Majauskiene Daiva , Karanauskiene Diana , Tilindiene Ilona
Published: April 2022

Background: The Olympic Games is one of the biggest sports events which should express and promote Olympic ideals. Aiming to generate more insights on the expression of Olympic Values, the Olympic Value Scale (OVS) was developed to assess how people perceive values in relation to the Olympic Games. The aim of the present study was to examine the validity and reliability of the Lithuanian version of OVS (LT-OVS). Methods: The scale construct validity and reliability was tested using a sample of 365 university students (mean age 22.02, SD = 6.58; 49.9% male). After the evaluation of the scale structure, convergent and discriminant validity as well as reliability of the scale were evaluated by assessing composite reliability and average variance extracted (AVE), examining the square root of the AVE. For further validity analysis, associations between the LT-OVS factors and other variables were examined. Results: The original OVS captures three dimensions, which are appreciation of diversity, friendly relations with others, and achievement in competition. Exploratory and confirmatory factor analyses confirmed the original three-factor structure of the OVS. The internal consistency values for all three subscales of the LT-OVS were 0.80 and higher. Convergent and discriminant validity criterions were met. Relations between the LT-OVS dimensions and attitudes towards fair play and Olympic Games were also revealed and discussed. Conclusions: This study makes a contribution by confirming the validity of the LT-OVS and encouraging future adaptation of it into other cultures and research on Olympic Values.
Relationship between Eye Blink Frequency and Incremental Exercise among Young Healthy Men
Authors: Paśko Wojciech , Zadarko Emilian , Krzeszowski Tomasz , Przednowek Krzysztof
Published: April 2022

The aim of the study was to verify the correlation between the frequency of blinking and aerobic physical exercise. The research subjects were 13 healthy man aged 23.3 ± 1 year. Measurements of the blink rate and eye closure times were performed during a progressive aerobic test on a cycle ergometer. During the test, power was gradually increased every minute by 25 W, starting from 50 W. Data acquisition involved using a GoPro camera mounted to the helmet of the research subject. The test continued until the research subject refused to continue. The subjects did not know the goal of the test, in order to ensure objectivity and obtain natural results. The largest number of statistically significant differences was observed between the initial stages and 250 W, as well as between 250 W and 325 W. The analysis showed no significant differences in blink rate, eye closure time, and single blink time in terms of heart rate ranges. Regression models were also determined for eye closure time, blink frequency, and single blink time. The analysis showed that blink frequency and eye closure time were determined by a group of factors (the value of cycle ergometer load power, heart rate, body weight, adipose tissue mass, fat-free mass, and total body water and body surface ratio).
Educational Gradients in Drinking Amount and Heavy Episodic Drinking among Working-Age Men and Women in Spain
Authors: Donat Marta , Barrio Gregorio , Guerras Juan-Miguel , Herrero Lidia , Pulido José , Belza María-José , Regidor Enrique
Published: April 2022

Alcohol-related harm decreases as socioeconomic position increases, although sometimes the opposite happens with alcohol intake. The objective was to know the educational gradient in monthly measures of drinking amount and heavy episodic drinking (HED) among people aged 25–64 years in Spain from 1997–2017. Such gradient was characterized with the relative percent change (PC) in drinking measures per year of education from generalized linear regression models after adjusting for age, year, region, marital status and immigration status. Among men, the PCs were significantly positive (p < 0.05) for prevalence of <21 g alcohol/day (2.9%) and 1–3 HED days (1.4%), and they were negative for prevalences of 21–40 g/day (−1.1%), >40 g/day (−6.0%) and ≥4 HED days (−3.2%), while among women they ranged from 3.6% to 5.7%. The gradient in prevalences of >40 g/day (men) and >20 g/day (women) was greatly attenuated after additionally adjusting for HED, while that of ≥4 HED days was only slightly attenuated after additionally adjusting for drinking amount. Among women, the gradients, especially in HED measures, seem steeper in 2009–2017 than in 1997–2007. Educational inequality remained after additional adjustment for income and occupation, although it decreased among women. These results can guide preventive interventions and help explain socioeconomic inequalities in alcohol-related harm.
Changes in Perceived Stress and Lifestyle Behaviors in Response to the COVID-19 Pandemic in The Netherlands: An Online Longitudinal Survey Study
Authors: Slurink Isabel A. L. , Smaardijk Veerle R. , Kop Willem J. , Kupper Nina , Mols Floortje , Schoormans Dounya , Soedamah-Muthu Sabita S.
Published: April 2022

The COVID-19 pandemic has substantial implications for physical and mental wellbeing. This study investigated changes, over time, in lifestyle behaviors and perceived stress during the initial phase of the pandemic and associations with COVID-19 symptoms, in the Dutch general population. An online longitudinal survey study was performed with pre-lockdown measurements in February, and subsequently in April and June 2020 (n = 259, mean age 59 ± 14 years, 59% women). Self-report questionnaires were used to assess weight, diet quality, physical activity, alcohol intake, and smoking. Perceived stress was measured using the validated perceived stress scale (PSS-10). The presence of COVID-19 symptoms (yes/no) was defined as fever, or >3 of the following symptoms: weakness/tiredness, muscle ache, dry cough, loss of smell/taste, and breathing difficulties. Data were analyzed using linear mixed models, adjusted for age, sex, educational level, marital status and (change in) employment status. Minimal increases over time were observed in alcohol intake (0.6 ± 0.7 to 0.7 ± 1.1 glasses/day, p = 0.001) and smoking (9.5 ± 8.7 to 10.9 ± 9.4 cigarettes/day among 10% smokers, p = 0.03), but other lifestyle behaviors remained stable. In April 2020, 15% reported COVID-19-related symptoms, and in June 2020, this was 10%. The presence of COVID-19 symptoms was associated with increased perceived stress (pinteraction = 0.003) and increased alcohol consumption (pinteraction = 0.03) over time. In conclusion, in this prospective study, COVID-19 symptoms were associated with increases in perceived stress and alcohol consumption. Future research on biopsychosocial determinants and underlying mechanisms of lifestyle changes, as a response to the COVID-19 pandemic, is needed.
Revealing Dynamic Spatial Structures of Urban Mobility Networks and the Underlying Evolutionary Patterns
Authors: Liu Chun , Chen Li , Yuan Quan , Wu Hangbin , Huang Wei
Published: April 2022

Urban space exhibits rich and diverse organizational structures, which is difficult to characterize and interpret. Modelling urban spatial structures in the context of mobility and revealing their underlying patterns in dynamic networks are key to understanding urban spatial structures and how urban systems work. Most existing methods overlook its temporal dimension and oversimplify its spatial heterogeneity, and it is challenging to address these complex properties using one single method. Therefore, we propose a framework based on temporal networks for modeling dynamic urban mobility structures. First, we cast aggregated traffic flows into a compact and informative temporal network for structure representation. Then, we explore spatial cluster substructures and temporal evolution patterns to acquire evolution regularities. Last, the capability of the proposed framework is examined by an empirical analysis based on taxi mobility networks. The experiment results enable to quantitatively depict urban space dynamics and effectively detect spatiotemporal heterogeneity in mobility networks.
Menthone Inhalation Alleviates Local and Systemic Allergic Inflammation in Ovalbumin-Sensitized and Challenged Asthmatic Mice
Authors: Su Yi-Hsuan , Lin Jin-Yuarn
Published: April 2022

Menthone is rich in Mentha × Piperita L. essential oil and it has anti-inflammatory properties; research shows that it is useful, via percutaneous absorption, in treating inflammation-related diseases. However, anti-allergic inflammatory effects of volatile menthone have not yet been used to treat allergic asthma, in vivo. We hypothesized that menthone inhalation may have anti-inflammatory and anti-allergic effects in patients with allergic asthma. Therefore, in our study, menthone inhalation was used to treat ovalbumin (OVA)-sensitized and challenged asthmatic mice. Allergic inflammation mediator changes in the lungs and airways, sera, splenocytes, and peritoneal macrophages of the mice were measured. Relative expression amounts of six receptor genes related to allergic inflammation of the lungs and airways were quantitated using a two-step real time quantitative polymerase chain reaction (qPCR). Results showed that menthone inhalation increased serum OVA-specific IgG2a/IgG1 and IgG2a/IgE ratios, increased Th1-type cytokine production in the bronchoalveolar lavage fluid, and decreased nitric oxide, protein, and eotaxin levels. Menthone inhalation inhibited mast cell and eosinophil degranulation, and chemokine (C-C motif) receptor 3 (Ccr3) gene expression amounts, but (relatively) increased Th1 cytokine secretion by splenocytes. Our results evidence that menthone inhalation alleviates local and systemic allergic inflammation in asthmatic mice.
Enhanced Magnetic Hyperthermia of Magnetoferritin through Synthesis at Elevated Temperature
Authors: Yu Jiacheng , Cao Changqian , Fang Fengjiao , Pan Yongxin
Published: April 2022

Iron oxide nanoparticles have attracted a great deal of research interest in recent years for magnetic hyperthermia therapy owing to their biocompatibility and superior thermal conversion efficiency. Magnetoferritin is a type of biomimetic superparamagnetic iron oxide nanoparticle in a ferritin cage with good monodispersity, biocompatibility, and natural hydrophilicity. However, the magnetic hyperthermic efficiency of this kind of nanoparticle is limited by the small size of the mineral core as well as its low synthesis temperature. Here, we synthesized a novel magnetoferritin particle by using a recombinant ferritin from the hyperthermophilic archaeon Pyrococcus furiosus as a template with high iron atom loading of 9517 under a designated temperature of 90 °C. Compared with the magnetoferritins synthesized at 45 and 65 °C, the one synthesized at 90 °C displays a larger average magnetite and/or maghemite core size of 10.3 nm. This yields an increased saturation magnetization of up to 49.6 emu g−1 and an enhanced specific absorption rate (SAR) of 805.3 W g−1 in an alternating magnetic field of 485.7 kHz and 49 kA m−1. The maximum intrinsic loss power (ILP) value is 1.36 nHm2 kg−1. These results provide new insights into the biomimetic synthesis of magnetoferritins with enhanced hyperthermic efficiency and demonstrate the potential application of magnetoferritin in the magnetic hyperthermia of tumors.
Neurotropic RNA Virus Modulation of Immune Responses within the Central Nervous System
Authors: Vazquez Christine , Jurado Kellie A.
Published: April 2022

The central nervous system (CNS) necessitates intricately coordinated immune responses to prevent neurological disease. However, the emergence of viruses capable of entering the CNS and infecting neurons threatens this delicate balance. Our CNS is protected from foreign invaders and excess solutes by a semipermeable barrier of endothelial cells called the blood–brain barrier. Thereby, viruses have implemented several strategies to bypass this protective layer and modulate immune responses within the CNS. In this review, we outline these immune regulatory mechanisms and provide perspectives on future questions in this rapidly expanding field.
Generation of a Library of Carbohydrate-Active Enzymes for Plant Biomass Deconstruction
Authors: Cardoso Vânia , Brás Joana L. A. , Costa Inês F. , Ferreira Luís M. A. , Gama Luís T. , Vincentelli Renaud , Henrissat Bernard , Fontes Carlos M. G. A.
Published: April 2022

In nature, the deconstruction of plant carbohydrates is carried out by carbohydrate-active enzymes (CAZymes). A high-throughput (HTP) strategy was used to isolate and clone 1476 genes obtained from a diverse library of recombinant CAZymes covering a variety of sequence-based families, enzyme classes, and source organisms. All genes were successfully isolated by either PCR (61%) or gene synthesis (GS) (39%) and were subsequently cloned into Escherichia coli expression vectors. Most proteins (79%) were obtained at a good yield during recombinant expression. A significantly lower number (p < 0.01) of proteins from eukaryotic (57.7%) and archaeal (53.3%) origin were soluble compared to bacteria (79.7%). Genes obtained by GS gave a significantly lower number (p = 0.04) of soluble proteins while the green fluorescent protein tag improved protein solubility (p = 0.05). Finally, a relationship between the amino acid composition and protein solubility was observed. Thus, a lower percentage of non-polar and higher percentage of negatively charged amino acids in a protein may be a good predictor for higher protein solubility in E. coli. The HTP approach presented here is a powerful tool for producing recombinant CAZymes that can be used for future studies of plant cell wall degradation. Successful production and expression of soluble recombinant proteins at a high rate opens new possibilities for the high-throughput production of targets from limitless sources.
Benefits and Implications of Resveratrol Supplementation on Microbiota Modulations: A Systematic Review of the Literature
Authors: Inchingolo Alessio Danilo , Malcangi Giuseppina , Inchingolo Angelo Michele , Piras Fabio , Settanni Vito , Garofoli Grazia , Palmieri Giulia , Ceci Sabino , Patano Assunta , De Leonardis Nicole , Di Pede Chiara , Montenegro Valentina , Azzollini Daniela , Garibaldi Maria Grazia , Kruti Zamira , Tarullo Antonella , Coloccia Giovanni , Mancini Antonio , Rapone Biagio , Semjonova Alexandra , Hazballa Denisa , D’Oria Maria Teresa , Jones Megan , Macchia Luigi , Bordea Ioana Roxana , Scarano Antonio , Lorusso Felice , Tartaglia Gianluca Martino , Maspero Cinzia , Del Fabbro Massimo , Nucci Ludovica , Ferati Kenan , Ferati Arberesha Bexheti , Brienza Nicola , Corriero Alberto , Inchingolo Francesco , Dipalma Gianna
Published: April 2022

Resveratrol is a polyphenol that has been shown to possess many applications in different fields of medicine. This systematic review has drawn attention to the axis between resveratrol and human microbiota, which plays a key role in maintaining an adequate immune response that can lead to different diseases when compromised. Resveratrol can also be an asset in new technologies, such as gene therapy. PubMed, Cochrane Library, Scopus, Web of Science, and Google Scholar were searched to find papers that matched our topic dating from 1 January 2017 up to 18 January 2022, with English-language restriction using the following Boolean keywords: (“resveratrol” AND “microbio*”). Eighteen studies were included as relevant papers matching the purpose of our investigation. Immune response, prevention of thrombotic complications, microbiota, gene therapy, and bone regeneration were retrieved as the main topics. The analyzed studies mostly involved resveratrol supplementation and its effects on human microbiota by trials in vitro, in vivo, and ex vivo. The beneficial activity of resveratrol is evident by analyzing the changes in the host’s genetic expression and the gastrointestinal microbial community with its administration. The possibility of identifying individual microbial families may allow to tailor therapeutic plans with targeted polyphenolic diets when associated with microbial dysbiosis, such as inflammatory diseases of the gastrointestinal tract, degenerative diseases, tumors, obesity, diabetes, bone tissue regeneration, and metabolic syndrome.
Interactions of Liberibacter Species with Their Psyllid Vectors: Molecular, Biological and Behavioural Mechanisms
Authors: Mishra Sapna , Ghanim Murad
Published: April 2022

Liberibacter is a group of plant pathogenic bacteria, transmitted by insect vectors, psyllids (Hemiptera: Psylloidea), and has emerged as one of the most devastating pathogens which have penetrated into many parts of the world over the last 20 years. The pathogens are known to cause plant diseases, such as Huanglongbing (citrus greening disease), Zebra chip disease, and carrot yellowing, etc., threatening some very important agricultural sectors, including citrus, potato and others. Candidatus Liberibacter asiaticus (CLas), the causative agent of citrus greening disease, is one of the most important pathogens of this group. This pathogen has infected most of the citrus trees in the US, Brazil and China, causing tremendous decline in citrus productivity, and, consequently, a severely negative impact on economic and personnel associated with citrus and related industries in these countries. Like other members in this group, CLas is transmitted by the Asian citrus psyllid (ACP, Diaphorina citri) in a persistent circulative manner. An additional important member of this group is Ca. L. solanacearum (CLso), which possesses nine haplotypes and infects a variety of crops, depending on the specific haplotype and the insect vector species. Ongoing pathogen control strategies, that are mainly based on use of chemical pesticides, lack the necessary credentials of being technically feasible, and environmentally safe. For this reason, strategies based on interference with Liberibacter vector transmission have been adopted as alternative strategies for the prevention of infection by these pathogens. A significant amount of research has been conducted during the last 10-15 years to understand the aspects of transmission of these bacterial species by their psyllid vectors. These research efforts span biological, ecological, behavioural and molecular aspects of Liberibacter–psyllid interactions, and will be reviewed in this manuscript. These attempts directed towards devising new means of disease control, endeavoured to explore alternative strategies, instead of relying on using chemicals for reducing the vector populations, which is the sole strategy currently employed and which has profound negative effects on human health, beneficial organisms and the environment.
Risk Stratification and Clinical Characteristics of Patients with Late Recurrence of Melanoma (>10 Years)
Authors: Reschke Robin , Dumann Konstantin , Ziemer Mirjana
Published: April 2022

Background: Most patients with high-risk melanomas develop metastasis within the first few years after diagnosis. However, late recurrence of melanoma is seen in patients that metastasize more than 10 years after the primary diagnosis; a metastasis after 15 years is considered an ultra-late recurrence. It is critical to better understand the clinical and biological characteristics of this subset of melanoma patients in order to offer an individual treatment plan and prevent metastasis. Methods: We retrospectively analyzed melanoma patients with recurrence ≥10 years after a primary diagnosis documented between 1993 and 2012 at the skin cancer center of the University Medical Center Leipzig, Germany. We conducted a comprehensive review of the literature and compared the results with our data. Available archived primary melanoma tissue was investigated with a seven-marker immunohistochemical signature (immunoprint®) previously validated to reliably identify high-risk patients within stages IB-III. Results: Out of 36 analyzed patients, a third metastasized ultra-late (≥15 years). The mean age at initial diagnosis was 51 years, with women being diagnosed comparatively younger than men. The largest proportion of patients with late recurrence had primary melanomas located on the trunk. The immunoprint® signature of the available primary melanomas allowed the accurate prediction of a high risk. However, it is difficult to draw a definitive conclusion from the small number of cases that could be analyzed with immunoprint® (n = 2) in this study. Apart from the primary tumor characteristics, the laboratory values at time of metastasis, comorbidities and outcome are also shown. Conclusion: Late and ultra-late recurrent melanomas seem not to differ from melanomas in general, apart from a distinctly higher proportion of lower leg localizations in ultra-late recurrent melanomas. The immunoprint® signature may help to identify high-risk primary tumors at the time of initial diagnosis. However, apart from the risk profile of the primary tumor, it seems that individual immune surveillance can control residual tumor cells for more than a decade. Advanced age and increasing comorbidities may contribute to a disturbed immunological balance.