bluuebunny/crossref_metadata_2025 huggingface dataset:
Modeling Superconducting Critical Temperature of 122-Iron-Based Pnictide Intermetallic Superconductor Using a Hybrid Intelligent Computational Method
Authors: Akomolafe Oluwatobi , Owolabi Taoreed O. , Abd Rahman Mohd Amiruddin , Awang Kechik Mohd Mustafa , Yasin Mohd Najib Mohd , Souiyah Miloud
Published: August 2021

Structural transformation and magnetic ordering interplays for emergence as well as suppression of superconductivity in 122-iron-based superconducting materials. Electron and hole doping play a vital role in structural transition and magnetism suppression and ultimately enhance the room pressure superconducting critical temperature of the compound. This work models the superconducting critical temperature of 122-iron-based superconductor using tetragonal to orthorhombic lattice (LAT) structural transformation during low-temperature cooling and ionic radii of the dopants as descriptors through hybridization of support vector regression (SVR) intelligent algorithm with particle swarm (PS) parameter optimization method. The developed PS-SVR-RAD model, which utilizes ionic radii (RAD) and the concentrations of dopants as descriptors, shows better performance over the developed PS-SVR-LAT model that employs lattice parameters emanated from structural transformation as descriptors. Using the root mean square error (RMSE), coefficient of correlation (CC) and mean absolute error as performance measuring criteria, the developed PS-SVR-RAD model performs better than the PS-SVR-LAT model with performance improvement of 15.28, 7.62 and 72.12%, on the basis of RMSE, CC and Mean Absolute Error (MAE), respectively. Among the merits of the developed PS-SVR-RAD model over the PS-SVR-LAT model is the possibility of electrons and holes doping from four different dopants, better performance and ease of model development at relatively low cost since the descriptors are easily fetched ionic radii. The developed intelligent models in this work would definitely facilitate quick and precise determination of critical transition temperature of 122-iron-based superconductor for desired applications at low cost with experimental stress circumvention.
Analysis of the Compression Behaviour of Reinforced Photocurable Materials Used in Additive Manufacturing Processes Based on a Mask Image Projection System
Authors: Bonada Jordi , Barcelona Pol , Casafont Miquel , Pons Josep Maria , Padilla Jose Antonio , Xuriguera Elena
Published: August 2021

Mask image projection based on stereolithography is an additive manufactured technology based on a Frontal Photopolymerization Process. Therefore, photocurable resins are used to build-up parts layer by layer. In this paper, alumina particles have been used as a reinforcement filler in order to improve the material stress-strain behaviour. In addition, the increment of the photoconversion ratio is a key factor to enhance the mechanical properties. Consequently, a numerical model has been used to determine the optimal printing parameters to enhance the elastic mechanical properties of printed parts according to the characteristics of photocurable materials. Stable and homogeneous reinforced materials have been obtained with an alumina content ranging from 5 to 15 wt%. Furthermore, the compression behaviour of reinforced materials has been analysed by means of experimental tests. The results show an enhancement of mechanical properties after the addition of reinforcement fillers, obtaining a maximum improvement in 10 wt% of solid load content. Finally, the influence of the sample’s orientation on the construction platform has been discussed.
Microstructure and Hardness of Spark Plasma Sintered Inconel 625-NbC Composites for High-Temperature Applications
Authors: Graboś Adrian , Huebner Jan , Rutkowski Paweł , Zhang Shenghua , Kuo Yen-Ling , Kata Dariusz , Hayashi Shigenari
Published: August 2021

The study focuses on obtaining Inconel 625-NbC composites for high-temperature applications, e.g., jet engines, waste-to-energy combusting systems or gas engine turbines, and characterizing them in terms of their microstructure and hardness improvement. Synthesis was performed utilizing Spark Plasma Sintering (SPS) at 1150 °C under the load of 45 MPa in medium vacuum (under 10−3 MPa) for a total time of 60 min. Four sets of samples with different Inconel 625 to NbC weight ratios were prepared (5, 10, 20, and 30 wt.%), followed by a reference sample containing no ceramic reinforcement. Obtained materials were hot-rolled at 1150 °C with a 10% reduction step and later cut and polished to perform characterization utilizing scanning electron microscopy (SEM) equipped with energy dispersive spectroscopy (EDS) module and microhardness testing device equipped with Vickers indenter. Hardness was improved proportionally to NbC addition achieving an increase of up to 20% of reference values. Additional heat treatment was conducted on the hot-rolled samples at 1200 °C in an argon atmosphere to further observe the interaction between reinforcement and alloy. Their microstructure revealed the coarsening of precipitates within the metal matrix and partial reinforcement dissolution, which proved to be crucial to obtaining the highest quality composites with homogenous hardness improvement.
A Numerical Study for the Dirichlet Problem of the Helmholtz Equation
Authors: Sun Yao , Hao Shijie
Published: August 2021

In this paper, an effective numerical method for the Dirichlet problem connected with the Helmholtz equation is proposed. We choose a single-layer potential approach to obtain the boundary integral equation with the density function, and then we deal with the weakly singular kernel of the integral equation via singular value decomposition and the Nystrom method. The direct problem with noisy data is solved using the Tikhonov regularization method, which is used to filter out the errors in the boundary condition data, although the problems under investigation are well-posed. Finally, a few examples are provided to demonstrate the effectiveness of the proposed method, including piecewise boundary curves with corners.
Factors Influencing Passengers’ Satisfaction with the Light Rail Transit Service in Alpha Cities: Evidence from Kuala Lumpur, Malaysia Using Structural Equation Modelling
Authors: Ibrahim Ahmad Nazrul Hakimi , Borhan Muhamad Nazri , Mat Yazid Muhamad Razuhanafi , Rahmat Riza Atiq , Yukawa Sotaro
Published: August 2021

Policy makers and service providers must have a good understanding of the determinants of passengers’ satisfaction with light rail transit (LRT) in order to be able to formulate effective strategies that ensure passenger satisfaction, which would in turn help to retain existing passengers and attract new ones. This is seen as the most important determinant of the long-term financial performance of LRT service. In this context, the present study seeks to establish the influence of passenger expectation, perceived quality and perceived value on their satisfaction with the service provided. A total of 417 responses from a self-administrated questionnaire were collected from LRT passengers in Kuala Lumpur, Malaysia. The proposed model was tested using a structural equation model. The results proved the significant and direct effect of perceived quality and perceived value on passenger satisfaction. Of the factors, perceived quality is the most critical construct determining the satisfaction level of LRT passengers. The present study concludes by deriving the theoretical and managerial implications on the field of transport. This study provides beneficial information which helps service providers, authorities, policy makers, planners, and researchers formulate effective strategies to increase the use of LRT service, especially in Kuala Lumpur, Malaysia, and other alpha and metropolitan cities in general.
Time-Optimal Control for Semilinear Stochastic Functional Differential Equations with Delays
Authors: Kang Yong Han , Jeong Jin-Mun
Published: August 2021

The purpose of this paper is to find the time-optimal control to a target set for semilinear stochastic functional differential equations involving time delays or memories under general conditions on a target set and nonlinear terms even though the equations contain unbounded principal operators. Our research approach is to construct a fundamental solution for corresponding linear systems and establish variations of a constant formula of solutions for given stochastic equations. The existence result of time-optimal controls for one point target set governed by the given semilinear stochastic equation is also established.
A Method of Solubilizing and Concentrating Astaxanthin and Other Carotenoids
Authors: Hara Kiyotaka Y. , Yagi Shuwa , Hirono-Hara Yoko , Kikukawa Hiroshi
Published: August 2021

The valuable marine carotenoid, astaxanthin, is used in supplements, medicines and cosmetics. In this study, crustacyanin, an astaxanthin-binding protein, was used to solubilize and concentrate astaxanthin. The recombinant crustacyanin of European lobster spontaneously formed an inclusion body when it was over-expressed in Escherichia coli. In this study, fusing the NusA-tag to the crustacyanin subunits made it possible to express in a soluble fraction and solubilize astaxanthin in aqueous solution. By cutting off the NusA-tag, the crustacyanin subunits generated the pure insoluble form, and captured and concentrated astaxanthin. Overall, the attaching and releasing NusA-tag method has the potential to supply solubilized carotenoids in aqueous solution and concentrated carotenoids, respectively.
Evaluation of Aqueous Biphasic Electrophoresis System Based on Halide-Free Ionic Liquids for Direct Recovery of Keratinase
Authors: Kee Phei Er , Yim Hip Seng , Kondo Akihiko , Lan John Chi-Wei , Ng Hui Suan
Published: August 2021

Aqueous biphasic electrophoresis system (ABES) incorporates electric fields into the biphasic system to separate the target biomolecules from crude feedstock. Ionic liquid (IL) is regarded as an excellent candidate as the phase-forming components for ABES because of the great electrical conductivity, which can promote the electromigration of biomolecules in ABES, and thereby enhances the separation efficiency of the target biomolecules from crude feedstock. The application of electric fields to the conventional biphasic system expedites the phase settling time of the biphasic system, which eases the subsequent scaling-up steps and reduces the overall processing time of the recovery process. Alkyl sulphate-based IL is a green and economical halide-free surfactant when compared to the other halide-containing IL. The feasibility of halide-free IL-based ABES to recover Kytococcus sedentarius TWHK01 keratinase was studied. Optimum partition coefficient (Ke = 7.53 ± 0.35) and yield (YT = 80.36% ± 0.71) were recorded with IL-ABES comprised of 15.0% (w/w) [EMIM][ESO4], 20.0% (w/w) sodium carbonate and 15% (w/w) crude feedstock. Selectivity (S) of 5.75 ± 0.27 was obtained with the IL-ABES operated at operation time of 5 min with 10 V voltage supplied. Halide-free IL is proven to be a potential phase-forming component of IL-ABES for large-scale recovery of keratinase.
Linking the Tuneability and Defouling of Electrically Conductive Polyaniline/Exfoliated Graphite Composite Membranes
Authors: Xu Lili , Wang Kunpeng , Wang Jun , Patterson Darrell Alec
Published: August 2021

Stimuli responsive membranes, which are able to respond to environmental stimuli, are attracting ever-increasing interests. In this study, we blended exfoliated graphite (EG) into the polyaniline (PANI) and developed PANI/EG composite membranes. The properties of the new generated membranes, especially the stimuli response properties (e.g., electrical tuneability, deformation), were studied. The fouling removal ability of the membrane under applied electrical potential was also investigated by using bovine serum albumin (BSA) as a model foulant. A flat membrane with defect-free surface and good adhesion to the support layer was formed by non-solvent induced phase separation method. The electrical conductivity of the formed PANI/EG composite membrane was (5.10 ± 0.27) ×10−4 S cm−1. The dynamic droplet penetration rate through the membranes showed an increase under applied electrical potential, which gives a preliminary quantitative indication of the electrical tuneability of the membranes. The membrane deformation appeared at a fast response under applied potential and recovered to its original position immediately when removing the applied potential. The application of electrical potential led to the removal of BSA foulant from the membrane surface as indicated by the increase in permeance of the fouled membrane on cleaning with 46.2% flux recovery ratio and increased BSA concentration in the wash solution. The electrically conductive PANI/EG composite membranes are able to respond to electrical stimuli, enabling a new range of potential applications including externally tuneability and in situ removal and control of fouling.
Miniature Ultralight Deformable Squama Mechanics and Skin Based on Piezoelectric Actuation
Authors: Lu Xiang , Xi Xiang , Lu Kun , Wang Chengxiang , Chen Xiang , Wu Yulie , Wu Xuezhong , Xiao Dingbang
Published: August 2021

A miniature deformable squama mechanics based on piezoelectric actuation inspired by the deformable squama is proposed in this paper. The overall size of the mechanics is 16 mm × 6 mm × 6 mm, the weight is only 140 mg, the deflection angle range of the mechanical deformation is −15°~45°, and the mechanical deformation is controllable. The small-batch array processing of the miniature deformable squama mechanics, based on the stereoscopic process, laid the technological foundation for applying the deformed squama array arrangement. We also designed and manufactured a small actuation control boost circuit and a mobile phone piezoelectric control assistant application that makes it convenient to perform short-range non-contact control of the deformation of the squama. The proposed system arranges the deformed squamae into groups to form the skin and controlls the size and direction of the signals input to each group of the squama array, thereby making the skin able to produce different shapes to create deformable skin.
Virulence Pattern Analysis of Three Listeria monocytogenes Lineage I Epidemic Strains with Distinct Outbreak Histories
Authors: Wagner Martin , Slaghuis Jörg , Göbel Werner , Vázquez-Boland José Antonio , Rychli Kathrin , Schmitz-Esser Stephan
Published: August 2021

Strains of the food-borne pathogen Listeria (L.) monocytogenes have diverse virulence potential. This study focused on the virulence of three outbreak strains: the CC1 strain PF49 (serovar 4b) from a cheese-associated outbreak in Switzerland, the clinical CC2 strain F80594 (serovar 4b), and strain G6006 (CC3, serovar 1/2a), responsible for a large gastroenteritis outbreak in the USA due to chocolate milk. We analysed the genomes and characterized the virulence in vitro and in vivo. Whole-genome sequencing revealed a high conservation of the major virulence genes. Minor deviations of the gene contents were found in the autolysins Ami, Auto, and IspC. Moreover, different ActA variants were present. Strain PF49 and F80594 showed prolonged survival in the liver of infected mice. Invasion and intracellular proliferation were similar for all strains, but the CC1 and CC2 strains showed increased spreading in intestinal epithelial Caco2 cells compared to strain G6006. Overall, this study revealed long-term survival of serovar 4b strains F80594 and PF49 in the liver of mice. Future work will be needed to determine the genes and molecular mechanism behind the long-term survival of L. monocytogenes strains in organs.
Engagement of CEACAM1 by Helicobacter pylori HopQ Is Important for the Activation of Non-Canonical NF-κB in Gastric Epithelial Cells
Authors: Taxauer Karin , Hamway Youssef , Ralser Anna , Dietl Alisa , Mink Karin , Vieth Michael , Singer Bernhard B. , Gerhard Markus , Mejías-Luque Raquel
Published: August 2021

The gastric pathogen Helicobacter pylori infects half of the world’s population and is a major risk factor for gastric cancer development. In order to attach to human gastric epithelial cells and inject the oncoprotein CagA into host cells, H. pylori utilizes the outer membrane protein HopQ that binds to the cell surface protein CEACAM, which can be expressed on the gastric mucosa. Once bound, H. pylori activates a number of signaling pathways, including canonical and non-canonical NF-κB. We investigated whether HopQ–CEACAM interaction is involved in activating the non-canonical NF-κB signaling pathway. Different gastric cancer cells were infected with the H. pylori wild type, or HopQ mutant strains, and the activation of non-canonical NF-κB was related to CEACAM expression levels. The correlation between CEACAM levels and the activation of non-canonical NF-κB was confirmed in human gastric tissue samples. Taken together, our findings show that the HopQ–CEACAM interaction is important for activation of the non-canonical NF-κB pathway in gastric epithelial cells.
First Genome Description of Providencia vermicola Isolate Bearing NDM-1 from Blood Culture
Authors: Lupande-Mwenebitu David , Khedher Mariem Ben , Khabthani Sami , Rym Lalaoui , Phoba Marie-France , Nabti Larbi Zakaria , Lunguya-Metila Octavie , Pantel Alix , Lavigne Jean-Philippe , Rolain Jean-Marc , Diene Seydina M.
Published: August 2021

In this paper, we describe the first complete genome sequence of Providencia vermicola species, a clinical multidrug-resistant strain harboring the New Delhi Metallo-β-lactamase-1 (NDM-1) gene, isolated at the Kinshasa University Teaching Hospital, in Democratic Republic of the Congo. Whole genome sequencing of an imipenem-resistant clinical Gram-negative P. vermicola P8538 isolate was performed using MiSeq and Gridion, and then complete genome analysis, plasmid search, resistome analysis, and comparative genomics were performed. Genome assembly resulted in a circular chromosome sequence of 4,280,811-bp and 40.80% GC and a circular plasmid (pPV8538_NDM-1) of 151,684-bp and 51.93%GC, which was identified in an Escherichia coli P8540 strain isolated in the same hospital. Interestingly, comparative genomic analysis revealed multiple sequences acquisition within the P. vermicola P8538 chromosome, including three complete prophages, a siderophore biosynthesis NRPS cluster, a Type VI secretion system (T6SS), a urease gene cluster, and a complete Type-I-F CRISPR-Cas3 system. Β-lactamase genes, including blaCMY-6 and blaNDM-1, were found on the recombinant plasmid pPV8538_NDM-1, in addition to other antibiotic resistance genes such as rmtC, aac6’-Ib3, aacA4, catA1, sul1, aac6’-Ib-cr, tetA, and tetB. Genome comparison with Providencia species revealed 82.95% of average nucleotide identity (ANI), with P. stuartii species exhibiting 90.79% of proteome similarity. We report the first complete genome of P. vermicola species and for the first time the presence of the blaNDM-1 gene in this species. This work highlights the need to improve surveillance and clinical practices in DR Congo in order to reduce or prevent the spread of such resistance.
Modulation of the Mucosa-Associated Microbiome Linked to the PTPN2 Risk Gene in Patients with Primary Sclerosing Cholangitis and Ulcerative Colitis
Authors: Denoth Luisa , Juillerat Pascal , Kremer Andreas E. , Rogler Gerhard , Scharl Michael , Yilmaz Bahtiyar , Bluemel Sena ,   on behalf of the Swiss IBD Cohort Study
Published: August 2021

Gut microbiota appears to be involved in the pathogenesis of primary sclerosing cholangitis (PSC). The protein tyrosine phosphatase nonreceptor 2 (PTPN2) gene risk variant rs1893217 is associated with gut dysbiosis in inflammatory bowel disease (IBD), and PTPN2 was mentioned as a possible risk gene for PSC. This study assessed the microbial profile of ulcerative colitis (UC) patients with PSC and without PSC (non-PSC). Additionally, effects of the PTPN2 risk variant were assessed. In total, 216 mucosal samples from ileum, colon, and rectum were collected from 7 PSC and 42 non-PSC patients, as well as 28 control subjects (non-IBD). The microbial composition was derived from 16S rRNA sequencing data. Overall, bacterial richness was highest in PSC patients, who also had a higher relative abundance of the genus Roseburia compared to non-PSC, as well as Haemophilus, Fusobacterium, Bifidobacterium, and Actinobacillus compared to non-IBD, as well as a lower relative abundance of Bacteroides compared to non-PSC and non-IBD, respectively. After exclusion of patients with the PTPN2 risk variant, Brachyspira was higher in PSC compared to non-PSC, while, solely in colon samples, Eubacterium and Tepidimonas were higher in PSC vs. non-IBD. In conclusion, this study underlines the presence of gut mucosa-associated microbiome changes in PSC patients and rather weakens the role of PTPN2 as a PSC risk gene.
Research on Strength Prediction Model and Microscopic Analysis of Mechanical Characteristics of Cemented Tailings Backfill under Fractal Theory
Authors: Deng Hongwei , Duan Tao , Tian Guanglin , Liu Yao , Zhang Weiyou
Published: August 2021

In order to further study the internal relationship between the microscopic pore characteristics and macroscopic mechanical properties of cemented tailings backfill (CTB), in this study, mine tailings and ordinary Portland cement (PC32.5) were selected as aggregate and cementing materials, respectively, and different additives (anionic polyacrylamide (APAM), lime and fly ash) were added to backfill samples with mass concentration of 74% and cement–sand ratios of 1:4, 1:6 and 1:8. After 28 days of curing, based on the uniaxial compressive strength test, nuclear magnetic resonance (NMR) porosity test and the fractal characteristics of pore structure, the relationships of the compressive strength with the proportion and fractal dimension of pores with different radii were analyzed. The uniaxial compressive strength prediction model of the CTB with the proportion of harmless pores and the fractal dimension of harmful pores as independent variables was established. The results show that the internal pores of the material are mainly the harmless and less harmful pores, and the sum of the average proportions of the two reaches 73.45%. Some characterization parameters of pore structure have a high correlation with the compressive strength. Among them, the correlation coefficients of compressive strength with the proportion of harmless pores and fractal dimension of harmful pores are 0.9219 and 0.9049, respectively. The regression results of the strength prediction model are significant, and the correlation coefficient is 0.9524. The predicted strength value is close to the actual strength value, and the predicted results are accurate and reliable.
Cu-Doped-ZnO Nanocrystals Induce Hepatocyte Autophagy by Oxidative Stress Pathway
Authors: Bai Qianyu , Wang Yeru , Duan Luoyan , Xu Xiaomu , Hu Yusheng , Yang Yue , Zhang Lei , Liu Zhaoping , Bao Huihui , Liu Tianlong
Published: August 2021

As a novel nanomaterial for cancer therapy and antibacterial agent, Cu-doped-ZnO nanocrystals (CZON) has aroused concern recently, but the toxicity of CZON has received little attention. Results of hematology analysis and blood biochemical assay showed that a 50 mg/kg dosage induced the increase in white blood cells count and that the concentration of alanine aminotransferase (ALT), superoxide dismutase (SOD), catalase (CAT), and Malonaldehyde (MDA) in the serum, liver, and lungs of the CZON group varied significantly from the control mice. Histopathological examinations results showed inflammation and congestion in the liver and lung after a single injection of CZON at 50 mg/kg. A transmission electron microscope (TEM) result manifested the autolysosome of hepatocyte of mice which received CZON at 50 mg/kg. The significant increase in LC3-II and decrease in p62 of hepatocyte in vivo could be seen in Western blot. These results indicated that CZON had the ability to induce autophagy of hepatocyte. The further researches of mechanism of autophagy revealed that CZON could produce hydroxyl radicals measured by erythrocyte sedimentation rate (ESR). The result of bio-distribution of CZON in vivo, investigated by ICP-OES, indicated that CZON mainly accumulated in the liver and two spleen organs. These results suggested that CZON can induce dose-dependent toxicity and autophagy by inducing oxidative stress in major organs. In summary, we investigated the acute toxicity and biological distribution after the intravenous administration of CZON. The results of body weight, histomorphology, hematology, and blood biochemical tests showed that CZON had a dose-dependent effect on the health of mice after a single injection. These results indicated that CZON could induce oxidative damage of the liver and lung by producing hydroxyl radicals at the higher dose.
Hesperidin Is a Potential Inhibitor against SARS-CoV-2 Infection
Authors: Cheng Fang-Ju , Huynh Thanh-Kieu , Yang Chia-Shin , Hu Dai-Wei , Shen Yi-Cheng , Tu Chih-Yen , Wu Yang-Chang , Tang Chih-Hsin , Huang Wei-Chien , Chen Yeh , Ho Chien-Yi
Published: August 2021

Hesperidin (HD) is a common flavanone glycoside isolated from citrus fruits and possesses great potential for cardiovascular protection. Hesperetin (HT) is an aglycone metabolite of HD with high bioavailability. Through the docking simulation, HD and HT have shown their potential to bind to two cellular proteins: transmembrane serine protease 2 (TMPRSS2) and angiotensin-converting enzyme 2 (ACE2), which are required for the cellular entry of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Our results further found that HT and HD suppressed the infection of VeroE6 cells using lentiviral-based pseudo-particles with wild types and variants of SARS-CoV-2 with spike (S) proteins, by blocking the interaction between the S protein and cellular receptor ACE2 and reducing ACE2 and TMPRSS2 expression. In summary, hesperidin is a potential TMPRSS2 inhibitor for the reduction of the SARS-CoV-2 infection.
Effects of Dietary Food Components on Cognitive Functions in Older Adults
Authors: Ozawa Hitoshi , Miyazawa Taiki , Miyazawa Teruo
Published: August 2021

Population aging has recently been an important issue as the number of elderly people is growing worldwide every year, and the extension of social security costs is financially costly. The increase in the number of elderly people with cognitive decline is a serious problem related to the aging of populations. Therefore, it is necessary to consider not only physical care but also cognitive patterns in the future care of older adults. Since food contains a variety of bioactive substances, dietary patterns may help improve age-related cognitive decline. However, the relationship between cognitive function and individual food components remains ambiguous as no clear efficacy or mechanism has been confirmed. Against this background, this review summarizes previous reports on the biological process of cognitive decline in the elderly and the relationship between individual compounds in foods and cognitive function, as well as the role of individual components of food in cognitive function, in the following order: lipids, carotenoids, vitamins, phenolic compounds, amino acids, peptides, and proteins. Based on the research presented in this review, a proper diet that preserves cognitive function has the potential to improve age-related cognitive decline, Alzheimer’s disease, and Parkinson’s disease. Hopefully, this review will help to trigger the development of new foods and technologies that improve aging and cognitive functions and extend the healthy life span.
Mediterranean Diet Adherence in a Sample of Italian Adolescents Attending Secondary School—The “#facciamoComunicAzione” Project
Authors: Dinu Monica , Lotti Sofia , Pagliai Giuditta , Pisciotta Livia , Zavatarelli Mariacarla , Borriello Matilde , Solinas Roberto , Galuffo Roberto , Clavarino Alessandro , Acerra Ettore , Sofi Francesco ,   on behalf of the Working Group of the “#facciamoComunicAzione” Project
Published: August 2021

Our aim was to assess adherence to the Mediterranean diet in a group of 726 secondary school students (336 girls, 390 boys) who completed the web-based Medi-Lite questionnaire simultaneously, during school hours, at the “Istituto Professionale per l’Enogastronomia e l’Ospitalità Alberghiera Marco Polo” in Genoa, Italy. The mean adherence score was 9.28 ± 2.29, with significantly (p = 0.017) higher values in girls (9.5 ± 2.2) than boys (9.1 ± 2.4). As to the individual food components of the Medi-Lite score, 84% of students reported non-optimal consumption (i.e., the choice that yielded ≤ 1 point) of meat and meat products, and over 50% reported non-optimal consumption of vegetables, legumes, dairy products, and fish. Significant differences between girls and boys were observed for fruit (p = 0.003), cereals (p < 0.001), meat and meat products (p < 0.001), and dairy products (p = 0.003). By conducting a principal component analysis, we observed that Medi-Lite items on the consumption of some animal products (meat and meat products and dairy products) and some plant products (fruit, vegetables, and legumes) generated contrasting patterns of responses, denoting excessive consumption in the first case and underconsumption in the second. This result suggests the need for effective actions to promote healthy eating habits in young people.
Healthy Eating Is Associated with Sarcopenia Risk in Physically Active Older Adults
Authors: Papaioannou Konstantinos-Georgios , Nilsson Andreas , Nilsson Lena Maria , Kadi Fawzi
Published: August 2021

Healthy Diet and physical activity may play important roles in the maintenance of muscle health during aging. The aim of the present study was to explore the impact of adherence to healthy dietary patterns on sarcopenia risk in a sample of physically active older men and women, while considering adherence to guidelines on muscle strengthening activities (MSA) and protein intake. Based on a sample of 191 physically active men and women (65–70 years), dietary intake was assessed using a 90-items food-frequency-questionnaire (FFQ) and Healthy Diet Score (HDS) was calculated. Physical activity was assessed by accelerometry and self-report. A sarcopenia risk score (SRS) was derived based on three indicators of muscle health: muscle mass was assessed using bioelectrical impedance and handgrip strength and 5 times sit-to-stand (5-STS) were determined by standardized procedures. Analysis of covariance (ANCOVA) was used to examine differences in SRS and its components across sex-specific tertiles of HDS, with adjustments for covariates including total energy intake, protein intake and MSA. A significant main effect (p < 0.05) of HDS on SRS was observed, where those belonging to the highest HDS tertile had lower SRS compared to those in the lowest tertile. A corresponding significant effect was observed for 5-STS performance, with better performance in those with the highest HDS adherence compared to those with the lowest. The present study supports guidelines emphasizing diet quality beyond amounts of macro- and micronutrients in the prevention of age-related deterioration of muscle health. Importantly, the benefits from healthy dietary patterns are evident in older adults who already adhere to guidelines for health-enhancing physical activity.
Multi-Level Fibrotomy for Pediatric Patients with Cerebral Palsy: A Cohort Study
Authors: Costici Pier Francesco , De Salvatore Sergio , Russo Rosa , Oggiano Leonardo , Burrofato Aaron , Donati Fabrizio , Ruzzini Laura
Published: August 2021

Muscle retraction in Cerebral Palsy (CP) often requires surgical treatment. Multilevel procedures (using open or percutaneous techniques) are commonly performed in the ambulant patient with CP. The necessity to find new surgical techniques, reduce postoperative discomfort, and accelerate the healing process and rehabilitation is mandatory for these patients. A retrospective cohort study with 189 pediatric patients with CP was performed. The multilevel gradual fibrotomy of Ulzibat was modified using an ophthalmic knife. No significant complications were reported using our technique. Opioid drugs were not necessary, and casting time was reduced at the first 24 h. A significant Range of Motion recovery was assessed post-operatory and maintained at the last follow-up. Mean days of hospitalization were 2.2. The mean follow-up was 39 months (6–64 months). The modified multilevel fibrotomy reduces postoperative pain with easier patient management, resulting in a faster discharge from the hospital. However, the retrospective nature and the lack of a control group of the present study did not allow the authors to report significant results. Further studies with longer follow-up are in progress to obtain more certain data that confirm our preliminary results.
Summer Sores Secondary to a Hoof Crack in an Andalusian Stallion
Authors: Palozzo Adriana , Traversa Donato , Marruchella Giuseppe , Celani Gianluca , Morelli Simone , Petrizzi Lucio
Published: August 2021

Cutaneous habronemosis in horses is caused by larvae of the spirurid nematodes Habronema microstoma and Habronema muscae. These lesions, also known as “summer sores’’, are often severe and disfiguring. Although Habronema-caused lesions at the coronary grooves have been described, cases of hoof cracks with secondary summer sores have never been reported. The present case describes clinic-pathological and surgical features of a quarter crack case complicated by cutaneous habronemosis at the dermal layers. A 15-year-old, Andalusian stallion was referred to the Veterinary Teaching Hospital of the University of Teramo because of a mass of the hoof and a severe lameness. The clinical examination revealed an exuberant granulation tissue protruding from a full thickness vertical quarter crack. The mass was surgically removed, and subjected to histopathological, microbiological, and parasitological analyses. A copromicroscopic examination was also performed. The feces scored PCR positive for H. muscae, while the skin for both H. microstoma and H. muscae, thus confirming the primary role of Habronema in causing the hoof mass. This is the first description of a hoof wall crack complicated by summer sores, with simultaneous gastric habronemosis. This case confirms that a prompt diagnosis during fly activity is imperative for an efficacious treatment and a timely prevention of disfiguring summer sores.
Association between Olaparib Exposure and Early Toxicity in BRCA-Mutated Ovarian Cancer Patients: Results from a Retrospective Multicenter Study
Authors: Velev Maud , Puszkiel Alicja , Blanchet Benoit , de Percin Sixtine , Delanoy Nicolas , Medioni Jacques , Gervais Claire , Balakirouchenane David , Khoudour Nihel , Pautier Patricia , Leary Alexandra , Ajgal Zahra , Hirsch Laure , Goldwasser François , Alexandre Jerome , Beinse Guillaume
Published: August 2021

Factors associated with olaparib toxicity remain unknown in ovarian cancer patients. The large inter-individual variability in olaparib pharmacokinetics could contribute to the onset of early significant adverse events (SAE). We aimed to retrospectively analyze the pharmacokinetic/pharmacodynamic relationship for toxicity in ovarian cancer patients from “real life” data. The clinical endpoint was the onset of SAE (grade III/IV toxicity or dose reduction/discontinuation). Plasma olaparib concentration was assayed using liquid chromatography at any time over the dosing interval. Trough concentrations (CminPred) were estimated using a population pharmacokinetic model. The association between toxicity and clinical characteristics or CminPred was assessed by logistic regression and non-parametric statistical tests. Twenty-seven patients were included, among whom 13 (48%) experienced SAE during the first six months of treatment. Olaparib CminPred was the only covariate significantly associated with increased risk of SAE onset (odds ratio = 1.31, 95%CI = [1.10; 1.57], for each additional 1000 ng/mL). The ROC curve identified a threshold of CminPred = 2500 ng/mL for prediction of SAE onset (sensitivity/specificity 0.62 and 1.00, respectively). This study highlights a significant association between olaparib plasma exposure and SAE onset and identified the threshold of 2500 ng/mL trough concentration as potentially useful to guide dose adjustment in ovarian cancer patients.
Nanoparticulate Drug Delivery Strategies to Address Intestinal Cytochrome P450 CYP3A4 Metabolism towards Personalized Medicine
Authors: Zhang Rui Xue , Dong Ken , Wang Zhigao , Miao Ruimin , Lu Weijia , Wu Xiao Yu
Published: August 2021

Drug dosing in clinical practice, which determines optimal efficacy, toxicity or ineffectiveness, is critical to patients’ outcomes. However, many orally administered therapeutic drugs are susceptible to biotransformation by a group of important oxidative enzymes, known as cytochrome P450s (CYPs). In particular, CYP3A4 is a low specificity isoenzyme of the CYPs family, which contributes to the metabolism of approximately 50% of all marketed drugs. Induction or inhibition of CYP3A4 activity results in the varied oral bioavailability and unwanted drug-drug, drug-food, and drug-herb interactions. This review explores the need for addressing intestinal CYP3A4 metabolism and investigates the opportunities to incorporate lipid-based oral drug delivery to enable precise dosing. A variety of lipid- and lipid-polymer hybrid-nanoparticles are highlighted to improve drug bioavailability. These drug carriers are designed to target different intestinal regions, including (1) local saturation or inhibition of CYP3A4 activity at duodenum and proximal jejunum; (2) CYP3A4 bypass via lymphatic absorption; (3) pH-responsive drug release or vitamin-B12 targeted cellular uptake in the distal intestine. Exploitation of lipidic nanosystems not only revives drugs removed from clinical practice due to serious drug-drug interactions, but also provide alternative approaches to reduce pharmacokinetic variability.
Myxobacteria as a Source of New Bioactive Compounds: A Perspective Study
Authors: Bhat Mudasir Ahmad , Mishra Awdhesh Kumar , Bhat Mujtaba Aamir , Banday Mohammad Iqbal , Bashir Ommer , Rather Irfan A. , Rahman Safikur , Shah Ali Asghar , Jan Arif Tasleem
Published: August 2021

Myxobacteria are unicellular, Gram-negative, soil-dwelling, gliding bacteria that belong to class δ-proteobacteria and order Myxococcales. They grow and proliferate by transverse fission under normal conditions, but form fruiting bodies which contain myxospores during unfavorable conditions. In view of the escalating problem of antibiotic resistance among disease-causing pathogens, it becomes mandatory to search for new antibiotics effective against such pathogens from natural sources. Among the different approaches, Myxobacteria, having a rich armor of secondary metabolites, preferably derivatives of polyketide synthases (PKSs) along with non-ribosomal peptide synthases (NRPSs) and their hybrids, are currently being explored as producers of new antibiotics. The Myxobacterial species are functionally characterized to assess their ability to produce antibacterial, antifungal, anticancer, antimalarial, immunosuppressive, cytotoxic and antioxidative bioactive compounds. In our study, we have found their compounds to be effective against a wide range of pathogens associated with the concurrence of different infectious diseases.
An Iron Shield to Protect Epigallocatehin-3-Gallate from Degradation: Multifunctional Self-Assembled Iron Oxide Nanocarrier Enhances Protein Kinase CK2 Intracellular Targeting and Inhibition
Authors: Fasolato Luca , Magro Massimiliano , Cozza Giorgio , Sbarra Ferruccio , Molinari Simone , Novelli Enrico , Vianello Fabio , Venerando Andrea
Published: August 2021

Protein kinase CK2 is largely involved in cell proliferation and apoptosis and is generally recognized as an Achilles’ heel of cancer, being overexpressed in several malignancies. The beneficial effects of (−)-epigallocatechin-3-gallate (EGCG) in the prevention and treatment of several diseases, including cancer, have been widely reported. However, poor stability and limited bioavailability hinder the development of EGCG as an effective therapeutic agent. The combination of innovative nanomaterials and bioactive compounds into nanoparticle-based systems demonstrates the synergistic advantages of nanocomplexes as compared to the individual components. In the present study, we developed a self-assembled core-shell nanohybrid (SAMN@EGCG) combining EGCG and intrinsic dual-signal iron oxide nanoparticles (Surface Active Maghemite Nanoparticles). Interestingly, nano-immobilization on SAMNs protects EGCG from degradation, preventing its auto-oxidation. Most importantly, the nanohybrid was able to successfully deliver EGCG into cancer cells, displaying impressive protein kinase CK2 inhibition comparable to that obtained with the most specific CK2 inhibitor, CX-4945 (5.5 vs. 3 µM), thus promoting the phytochemical exploitation as a valuable alternative for cancer therapy. Finally, to assess the advantages offered by nano-immobilization, we tested SAMN@EGCG against Pseudomonas aeruginosa, a Gram-negative bacterium involved in severe lung infections. An improved antimicrobial effect with a drastic drop of MIC from 500 to 32.7 μM was shown.
Preface: The Role of Pharmacists in Palliative and End of Life Care
Authors: Wilson Eleanor
Published: August 2021

In this Special Issue exploring the role of pharmacists in palliative and end of life care, we sought articles that would shed light on the ways in which pharmacists could impact end of life care [...]
Nondestructive Structural Investigation of Yttria-Stabilized Zirconia Fiber Insulation Tile by Synchrotron X-ray In-Line Phase-Contrast Microtomography
Authors: Yao Shengkun , Liu Benxue , Ren Jing , Liu Jingwen , Qi Meili , Cai Yangjian
Published: August 2021

Zirconia (ZrO2) aerogels show excellent insulating performance and have been widely applied as a thermal protector in furnaces, nuclear reactors, and spacecraft. The nondestructive determination of their interior microstructure is significant for evaluating their mechanical and insulating performance. In this study, we performed nondestructive structural investigation of an yttria-stabilized ZrO2 fiber insulation tile using synchrotron X-ray in-line phase-contrast microtomography at a pixel resolution of 6.5 µm. Taking advantage of the edge enhancement of phase-contrast imaging, single yttria-stabilized ZrO2 fibers were clearly distinguished; furthermore, interior aggregates were nondestructively observed at this spatial resolution. This work demonstrates the advantages and potential of synchrotron X-ray microtomography for the structural analysis of porous ceramic materials. By combining higher-brilliance synchrotron radiation sources and CCD detectors with higher spatial and temporal resolutions, we anticipate that we can further understand the relationship between aerogel microstructure and function, especially under in-service conditions at high temperatures.
Sepal Identity of the Pappus and Floral Organ Development in the Common Dandelion (Taraxacum officinale; Asteraceae)
Authors: Vijverberg Kitty , Welten Monique , Kraaij Marjan , van Heuven Bertie Joan , Smets Erik , Gravendeel Barbara
Published: August 2021

The dry one-seeded fruits (cypselae) of the Asteraceae are often crowned with a pappus, an appendage of hairs or scales that assists in dispersal. It is generally assumed, but little investigated, that the pappus represents the outer floral whorl where the sepals are usually located. We analysed pappus–sepal homology in dandelions using micromorphological and floral gene expression analyses. We show that the pappus initiates from a ring primordium at the base of the corolla, heterochronic to the petals. Pappus parts form from this ring, with those in the alternipetalaous position usually being ahead in growth, referring to sepal identity. Tof-APETALLA1 expression increased during floret development and was highest in mature pappus. Tof-PISTILLATA expression was high and confined to the floral tissues containing the petals and stamens, consistent with expectations for sepals. Apart from the pappus, the dispersal structure of dandelion consists of the upper part of the fruit, the beak, which originates from the inner floral whorl. Thus, our results support the homology of the pappus with the sepals, but show that it is highly derived. Together with our floral stage definitions and verified qPCR reference genes, they provide a basis for evolution and development studies in dandelions and possibly other Asteraceae.
Advanced Applications for Protein and Compounds from Microalgae
Authors: Castiglia Daniela , Landi Simone , Esposito Sergio
Published: August 2021

Algal species still show unrevealed and unexplored potentiality for the identification of new compounds. Photosynthetic organisms represent a valuable resource to exploit and sustain the urgent need of sustainable and green technologies. Particularly, unconventional organisms from extreme environments could hide properties to be employed in a wide range of biotechnology applications, due to their peculiar alleles, proteins, and molecules. In this review we report a detailed dissection about the latest and advanced applications of protein derived from algae. Furthermore, the innovative use of modified algae as bio-reactors to generate proteins or bioactive compounds was discussed. The latest progress about pharmaceutical applications, including the possibility to obtain drugs to counteract virus (as SARS-CoV-2) were also examined. The last paragraph will survey recent cases of the utilization of extremophiles as bio-factories for specific protein and molecule production.
Surface Characterisation of PEEK and Dentin, Treated with Atmospheric Non-Thermal PDD Plasma, Applicable for Dental Chair-Side Procedures
Authors: Behnecke Marius , Steinert Vincent , Petersen Svea
Published: August 2021

This study investigates the suitability of Piezoelectric Direct Discharge Plasma as a tool for wetting behaviour modification of PEEK and dentin, and compares the results of this method with low-pressure plasma treatment and phosphoric acid etching. Static contact angle measurements were made, roughness was assessed using tactile measurement, and AFM and SEM images were taken. An optimum operating distance of ≤15 mm was determined for the plasma based on the water contact angle. Furthermore, it was demonstrated that despite only a fraction of the power, the PDD plasma also produces hydrophilic and nanostructured PEEK surfaces with a 38° water contact angle in the same plasma time. In contrast, the gold standard of dental surface modification of dentin—phosphoric acid etching—showed no measurable contact angle due to the exposed dentin tubules. Treatment with PDD plasma reduces the water contact angle of dentin from 65° to 43° and is not negative affected by water. Wet, PDD plasma-treated dentin samples show a water contact angle of only 26.5°. The dentin tubules exposed by chemical etching led to a significantly increased roughness. No comparable effect could be demonstrated for plasma treatment on dentin, but based on the contact angle measurements, a chemically strongly activated surface with strongly polar interaction behaviour can be assumed. The additional use of the PDD plasma technique to improve wetting could therefore have a positive effect on the adhesive bond between human dentin and polymeric dental restorative materials or, depending on the adhesive system, replace the etching process altogether.