bluuebunny/crossref_metadata_2025 huggingface dataset:
Willingness to Participate in a National Precision Medicine Cohort: Attitudes of Chronic Kidney Disease Patients at a Cleveland Public Hospital
Authors: Cooke Bailey Jessica N. , Crawford Dana C. , Goldenberg Aaron , Slaven Anne , Pencak Julie , Schachere Marleen , Bush William S. , Sedor John R. , O’Toole John F.
Published: June 2018

Multiple ongoing, government-funded national efforts longitudinally collect health data and biospecimens for precision medicine research with ascertainment strategies increasingly emphasizing underrepresented groups in biomedical research. We surveyed chronic kidney disease patients from an academic, public integrated tertiary care system in Cleveland, Ohio, to examine local attitudes toward participation in large-scale government-funded studies. Responses (n = 103) indicate the majority (71%) would participate in a hypothetical national precision medicine cohort and were willing to send biospecimens to a national repository and share de-identified data, but <50% of respondents were willing to install a phone app to track personal data. The majority of participants (62%) indicated that return of research results was very important, and the majority (54%) also wanted all of their research-collected health and genetic data returned. Response patterns did not differ by race/ethnicity. Overall, we found high willingness to participate among this Cleveland patient population already participating in a local genetic study. These data suggest that despite common perceptions, subjects from communities traditionally underrepresented in genetic research will participate and agree to store samples and health data in repositories. Furthermore, most participants want return of research results, which will require a plan to provide these data in a secure, accessible, and understandable manner.
Mechanical Characterization of Gres Porcelain and Low-Velocity Impact Numerical Modeling
Authors: Fragassa Cristiano , Vannucchi de Camargo Felipe , Pavlovic Ana , De Figueiredo Silveira Antonio Carlos , Minak Giangiacomo , Pérez Bergmann Carlos
Published: June 2018

The current investigation was conducted on gres porcelain stoneware, a robust, impermeable and aesthetically pleasing type of ceramic mainly used for flooring, characterizing its resistance to bending and low-velocity impact, both representative efforts to which flooring tiles are constantly subjected as a consequence of the fall of objects and microsubsidences. The mechanical characterization was made through experimental tests following an adapted low-velocity impact testing routine, and the model was by validated numerical simulation through the explicit code software LS-DYNA based on the Johnson–Holmquist constitutive material model. Specimens were tested before and after an annealing cycle industrially used to allow porcelain folding. The thermal treatment demonstrated to infer a decrease in mechanical resistance on the material, understood as a consequence of its elevated maximum temperature and fast cooling rate. The numerical model calibrated successfully allows predicting the behavior of gres porcelain before and after annealing against low-velocity impact.
Mesoporous Silicon Particles Favor the Induction of Long-Lived Humoral Responses in Mice to a Peptide-Based Vaccine
Authors: Navarro-Tovar Gabriela , Rocha-García Denisse , Wong-Arce Alejandra , Palestino Gabriela , Rosales-Mendoza Sergio
Published: June 2018

Vaccinology faces the challenge of developing improved immunization approaches that are able to induce long-term immunity with the desired Th profile according to the pathology. In this context, new vehicles for efficient antigen delivery that exert adjuvant effects play a critical role in addressing this goal. Herein, mesoporous silicon particles (PSiP) were assessed as carriers for a peptide-based vaccine targeting the receptor for advanced glycation end products (RAGE), which is a relevant receptor in Alzheimer´s disease and other diseases. A RAGE peptide was adsorbed onto PSiP (PSiP vaccine) and administered to BALB/c mice, leading to immune responses that were similar in magnitude to those induced by the soluble peptide. However, the response induced by PSiP lasted for a significantly longer period when compared with the behavior of the group immunized with the peptide alone. Therefore, PSiP are proposed as carriers to enhance immune memory, which is critical in vaccination. This study opens interesting perspectives related to the application of PSiP in vaccinology.
Phenotypic Variation during Biofilm Formation: Implications for Anti-Biofilm Therapeutic Design
Authors: Beitelshees Marie , Hill Andrew , Jones Charles H. , Pfeifer Blaine A.
Published: June 2018

Various bacterial species cycle between growth phases and biofilm formation, of which the latter facilitates persistence in inhospitable environments. These phases can be generally characterized by one or more cellular phenotype(s), each with distinct virulence factor functionality. In addition, a variety of phenotypes can often be observed within the phases themselves, which can be dependent on host conditions or the presence of nutrient and oxygen gradients within the biofilm itself (i.e., microenvironments). Currently, most anti-biofilm strategies have targeted a single phenotype; this approach has driven effective, yet incomplete, protection due to the lack of consideration of gene expression dynamics throughout the bacteria’s pathogenesis. As such, this article provides an overview of the distinct phenotypes found within each biofilm development phase and demonstrates the unique anti-biofilm solutions each phase offers. However, we conclude that a combinatorial approach must be taken to provide complete protection against biofilm forming bacterial and their resulting diseases.
Combined Effects of Texture and Grain Size Distribution on the Tensile Behavior of α-Titanium
Authors: Richeton Thiebaud , Wagner Francis , Chen Cai , Toth Laszlo S.
Published: June 2018

This work analyzes the role of both the grain size distribution and the crystallographic texture on the tensile behavior of commercially pure titanium. Specimens with different microstructures, especially with several mean grain sizes, were specifically prepared for that purpose. It is observed that the yield stress depends on the grain size following a Hall–Petch relationship, that the stress–strain curves have a tendency to form a plateau that becomes more and more pronounced with decreasing mean grain size and that the hardening capacity increases with the grain size. All these observations are well reproduced by an elasto-visco-plastic self-consistent model that incorporates grain size effects within a crystal plasticity framework where dislocations’ densities are the state variables. First, the critical resolved shear stresses are made dependent on the individual grain size through the addition of a Hall–Petch type term. Then, the main originality of the model comes from the fact that the multiplication of mobile dislocation densities is also made grain size dependent. The underlying assumption is that grain boundaries act mainly as barriers or sinks for dislocations. Hence, the smaller the grain size, the smaller the expansion of dislocation loops and thus the smaller the increase rate of mobile dislocation density is. As a consequence of this hypothesis, both mobile and forest dislocation densities increase with the grain size and provide an explanation for the grain size dependence of the transient low work hardening rate and hardening capacity.
Cyanopeptolins with Trypsin and Chymotrypsin Inhibitory Activity from the Cyanobacterium Nostoc edaphicum CCNP1411
Authors: Mazur-Marzec Hanna , Fidor Anna , Cegłowska Marta , Wieczerzak Ewa , Kropidłowska Magdalena , Goua Marie , Macaskill Jenny , Edwards Christine
Published: June 2018

Cyanopeptolins (CPs) are one of the most frequently occurring cyanobacterial peptides, many of which are inhibitors of serine proteases. Some CP variants are also acutely toxic to aquatic organisms, especially small crustaceans. In this study, thirteen CPs, including twelve new variants, were detected in the cyanobacterium Nostoc edaphicum CCNP1411 isolated from the Gulf of Gdańsk (southern Baltic Sea). Structural elucidation was performed by tandem mass spectrometry with verification by NMR for CP962 and CP985. Trypsin and chymotrypsin inhibition assays confirmed the significance of the residue adjacent to 3-amino-6-hydroxy-2-piperidone (Ahp) for the activity of the peptides. Arginine-containing CPs (CPs-Arg2) inhibited trypsin at low IC50 values (0.24–0.26 µM) and showed mild activity against chymotrypsin (IC50 3.1–3.8 µM), while tyrosine-containing CPs (CPs-Tyr2) were selectively and potently active against chymotrypsin (IC50 0.26 µM). No degradation of the peptides was observed during the enzyme assays. Neither of the CPs were active against thrombin, elastase or protein phosphatase 1. Two CPs (CP962 and CP985) had no cytotoxic effects on MCF-7 breast cancer cells. Strong and selective activity of the new cyanopeptolin variants makes them potential candidates for the development of drugs against metabolic disorders and other diseases.
The structure of myocardial nerve plexus of human auricle of the right atrium and its relation to myocardial ischemia, functional status of the heart, and age
Authors: Burkauskienė Aušra , Aželis Vidmantas , Senikienė Žibuoklė , Linonis Vitas , Ramanauskienė Irina
Published: June 2018

The aim of this study was to investigate and evaluate morphometrically the relationship between changes in the structure of myocardial nerve plexus of the right atrium auricle and myocardial ischemia, parameters reflecting functional status of the heart, and age. A total of 56 females and males aged 20–94 years were investigated. Ischemic heart disease group consisted of 39 persons (their mean age was 63.83±15.67 years). The control group comprised 17 persons (the mean age was 60.53±9.89 years). Control group consisted of deceased persons who according to the pathologic and anatomic examination were not diagnosed with cardiac pathology leading to heart lesions or overload. Ischemic heart disease group consisted of patients who underwent aorta-coronary artery bypass grafting surgery. In ischemic heart disease group, degree of coronary artery stenosis was evaluated as well as the major indicators reflecting the size of atria and formation of postinfarction scar. After examination, postinfarction scars were found in 18 (46.2%) persons; no scars were found in 21 (53.8%) persons. Neurohistochemical method and video microscopy were employed for the evaluation of quantitative changes in the structure of the myocardial nerve plexus. In ischemic heart disease group, the structures of nerve plexus occupied 5.0±1.0% of the area, perimeter was 10 488±2134 mm, and number of the structures was 2698±981; the same parameters in the control group were 6.0±1.4%, 13 008±443 mm, and 3469±1511, respectively. In persons with postinfarction scar, the number of nerve plexus structures was lower by 9.3%, area by 8.9%, perimeter by 9.7% on average as compared to ischemic heart disease group without a scar. Regression analysis did not reveal any statistically significant correlation between the degree of coronary artery stenosis and quantitive parameters of nerve plexus (P>0.05). Changes in quantitative parameters of nerve plexus were not related to compensatory dilation of the atria – echoscopy parameters of long and short axes. The results showed that the number, area, and perimeter of nerve plexus structures decreases at the same rate both in healthy subjects and patients with ischemic heart disease starting the fifth decade of life.
A randomized trial comparing hypofractionated and conventionally fractionated three-dimensional conformal external-beam radiotherapy for localized prostate adenocarcinoma: A report on the first-year biochemical response
Authors: Norkus Darius , Miller Albert , Plieskienė Aista , Janulionis Ernestas , Valuckas Konstantinas
Published: June 2018

Objective. This paper describes the first-year biochemical (prostate-specific antigen [PSA]) response of 91 irradiated patients enrolled in a single-institution randomized trial comparing hypofractionated (HFRT) and conventionally fractionated (CFRT) external beam radiotherapy. Material and methods. Forty-four patients in the CFRT treatment arm were irradiated with 74 Gy in 37 fractions (2 Gy per fraction), and 47 in the HFRT arm were treated with 57 Gy, given in 13 fractions of 3 Gy plus 4 fractions of 4.5 Gy. The clinical target volume includes the prostate and a base of seminal vesicles. The proportions of patients who reached PSA nadir (nPSA) lower than or equal to 1.0 ng/mL (nPSA1) and 0.5 ng/mL (nPSA05) were compared. Results. There were 2 non-cancer-related deaths (1 in the CFRT and 1 in the HFRT treatment arms). Biochemical relapse after irradiation was defined in five cases (3 in the CFRT and 2 in the HFRT treatment arms) during a 12-month follow-up. The remaining 84 patients were analyzed. The proportions of patients reaching nPSA1 were 50% and 54.5% in the CFRT and HFRT treatment arms, respectively (chi-square P=0.843). The percentages of patients reaching nPSA05 were 25% and 18.2%, respectively (chi-square P=0.621). The trends toward increasing proportions of biochemical responders (both nPSA1 and nPSA05) during 12 months after radiotherapy were observed, but the difference between trends for treatment arms did not reach a statistical significance. Conclusion. The preliminary results presented here demonstrate that HFRT schedule induces biochemical response rates comparable to those in the CFRT schedule during the first-year follow-up.
Treatment of Lewis lung carcinoma by photodynamic therapy and glucan from barley
Authors: Akramienė Dalia , Graželienė Gražina , Didžiapetrienė Janina , Kėvelaitis Egidijus
Published: June 2018

Objective. During the photodynamic treatment, complement system is activated and tumor cells are opsonized with iC3b fragment. β-glucans can enhance cytotoxicity of iC3bopsonized cells due to their specific interaction with complement receptor 3 (CR3; CD11b/CD18) on the surface of the effector cells. In contrast to microorganisms, tumor cells lack β-glucan as a surface component and cannot trigger complement receptor 3-dependent cellular cytotoxicity and initiate tumor-killing activity. This mechanism could be induced in the presence of β-glucans. This study aimed at determining the influence of coadministration of β-glucan from barley on the efficacy of photodynamic tumor therapy (PDT). Material and methods. C57 Bl/6 female mice bearing Lewis lung carcinoma were used throughout the study. Mice were randomized into groups (15 in each group) and exposed to the treatment with intravenous Photofrin injection (dose, 10 mg/kg) and after 24 h following laser illumination, or with oral administration of β-glucan from barley at a dose of 400 μg/mouse per day up to 5 days, or with their combination. Tumor growth dynamics and survival of the treated and untreated mice were monitored. Results. Tumor volume in all treated groups was significantly lower (P<0.001) than that in the control group. The most effective tumor growth suppression (P=0.033) was achieved in mice treated with combination of PDT and β-glucan from barley as compared with PDT alone. The best survival was achieved in the same group, but difference was not significant as compared to the control group (P=0.143) and to PDT alone group (P=0.319). Conclusions. The present study demonstrates that coadministration of β-glucan from barley can enhance efficacy of photodynamic therapy.
Psychometric properties of the World Health Organization Quality of Life 100 questionnaire in the middle-aged Lithuanian population of Kaunas city
Authors: Bacevičienė Miglė , Rėklaitienė Regina
Published: June 2018

This study aimed to identify psychometric properties of the World Health Organization Quality of Life 100 questionnaire. Material and methods. A random sample of 1403 Kaunas city men and women aged 35–64 years was examined in 2001–2002. Quality of life was assessed by the self-administered World Health Organization Quality of Life 100 questionnaire. Statistics. Scores of all domains and facets of the questionnaire were transformed to reflect a scale from 0 to 100. The validity and reliability of the questionnaire were evaluated by Pearson’s correlation coefficients and by Cronbach’s α. The relationship between the WHOQOL-100 domains and the two general items (G1 and G4) was assessed using linear regression analysis. Exploratory factor analysis was conducted to explore the factor structure of the data. Results. The mean scores of the World Health Organization Quality of Life 100 questionnaire domains ranged from 51.3±15.1 (overall quality of life) to 72.0±16.4 (level of independence domain). The instrument displayed acceptable Cronbach’s α (0.77–0.91) and test-retest reliability (0.64–0.89). The overall assessment of quality of life (G1) was most strongly associated with the environment (β=0.31), psychological (β=0.18), and social relationships (β=0.17) domains. Overall health (G4) showed the strongest association with the level of independence (β=0.34) and physical (β=0.18) domains. The principal component analysis revealed five-factor solution, which accounted for 57.7% of a total variance. Conclusion. In the Lithuanian population, the World Health Organization Quality of Life 100 questionnaire was found to be a suitable instrument for evaluating quality of life.
Nonpharmacological correction of low back pain by single or integrated means of medical rehabilitation and the evaluation of their effectiveness
Authors: Sakalauskienė Giedrė
Published: June 2018

Low back pain is a global worldwide problem. A great attention is given to correction of this health status by a wide range of rehabilitation specialists. Some single or integrated physical factors, physiotherapy, specific and nonspecific physical exercises, alternative methods of treatment, also the complex of multidisciplinary rehabilitation means are applied in the management of low back pain. The evidencebased data are analyzed in order to identify which nonpharmacological means are effective in pain correction; in addition, the effectiveness of various methods and models of low back pain management are compared in this article. Conclusions. Research data evaluating the impact effectiveness of single or integrated means of rehabilitation are very controversial. There are no evidence-based specific recommendations for the correction of this health status objectively assessing advantages of physiotherapy or physical factors and referring the definite indications of their prescription. It is thought that multidisciplinary rehabilitation is most effective in management of chronic low back pain. The positive results depend on the experience of a physician and other rehabilitation specialists. A patient’s motivation to participate in the process of pain control is very important. It is recommended to inform a patient about the effectiveness of administered methods. There is a lack of evidencebased trials evaluating the effectiveness of nonpharmacological methods of pain control in Lithuania. Therefore, the greater attention of researchers and administrative structures of health care should be given to this problem in order to develop the evidence-based guidelines for an effective correction of low back pain.
Comparative diagnostic value of computed tomography and contrast-enhanced ultrasonography in diagnosis of focal liver lesions
Authors: Žvinienė Kristina , Zaborienė Inga , Basevičius Algidas , Pundzius Juozas
Published: June 2018

Objective. The aim of the study was to evaluate focal liver lesions by computed tomography and contrast-enhanced ultrasonography and to compare their diagnostic values. Materials and methods. There were 67 patients, examined and treated in the Departments of Gastroenterology, Surgery, and Oncology, Hospital of Kaunas University of Medicine, during 2007 (study group). All the patients underwent contrast-enhanced ultrasonography and bolus computed tomography. Control group included 73 patients with focal hepatic lesions who were examined and treated in the Hospital of Kaunas University of Medicine during 2006. Focal hepatic lesions were detected and characterized by conventional ultrasonography and bolus computed tomography. The diagnosis was confirmed by biopsy or during surgery in both groups, and hemangiomas were confirmed by magnetic resonance imaging. Patients’ age varied from 20 to 80 years (there were 46 [68.7%] women and 21 [31.3%] men with a mean age of 55.85±13.417 years). The age of patients in the study group varied from 18 to 91 years (mean age, 60.81±16.059 years; out of 73 patients, 46 [63%] were women and 27 [37%] men). Results. The following was determined in the study group: hemangioma (n=18, 26.9%), focal nodular hyperplasia (n=4, 6%), adenoma (n=2, 3%), echinococcosis (n=2, 3%), hepatocellular carcinoma (n=11, 16.4%), cholangiocellular carcinoma (n=1, 1.5%), solitary metastasis (n=13, 19.4%), hepatic cyst (n=3, 4.5%), etc. The sensitivity and specificity of contrast-enhanced ultrasonography as compared with computed tomography in detecting and characterizing focal liver lesions was 44.2% and 46.7%, respectively; positive prognostic value was 74.2% and negative prognostic value was 19.4%. The sensitivity and specificity of conventional ultrasonography as compared with contrast-enhanced ultrasonography in detecting and characterizing focal liver lesions was 34.5% and 100%, respectively; positive prognostic value was 100% and negative prognostic value was 25%. Conclusions. Ultrasound contrast agents (SonoVue, Bracco®, Milan, Italy) definitely improve detection and characterization of focal liver lesions. Ultrasonography correlates with computed tomography and magnetic resonance imaging, particularly during arterial phase. The sensitivity of contrast-enhanced ultrasonography as compared with computed tomography in detecting and characterizing focal liver lesions was 74.2% and positive prognostic value was 44.2%; sensitivity of conventional ultrasonography as compared with contrastenhanced ultrasonography in detecting and characterizing focal liver lesions was 34.5% and positive prognostic value was 100%.
Efficacy, tolerability, and preference of mirtazapine orally disintegrating tablets in depressed patients: a 17-week naturalistic study in Lithuania
Authors: Danilevičiūtė Vita , Sveikata Audrius , Adomaitienė Virginija , Gumbrevičius Gintautas , Fokas Vidmantas , Sveikatienė Renata
Published: June 2018

Mirtazapine is an established antidepressant with well-documented efficacy demonstrated in controlled clinical trials. However, the gap between the results obtained in controlled clinical trials and everyday clinical practice exists. Therefore, the importance of naturalistic studies in psychiatry is becoming recognized. The aim of present naturalistic study was to acquire data on efficacy, safety, and preference of mirtazapine orally disintegrating tablets during a 17-week treatment of depression. This prospective, open-label, multicenter study in patients with mild to severe depression was conducted at 47 mental health centers of Lithuania by 78 psychiatrists. Patients were initially given 15 mg or 30 mg of mirtazapine orally disintegrating tablets; the maximum allowed dose was 45 mg per day. The primary efficacy measure was the total score on the Hamilton Depression Rating Scale-17 (HAMD-17), the Clinical Global Impression-Severity (CGI-S), and Clinical Global Impression-Improvement (CGI-I) scales. Tolerability was primarily measured by assessing the incidence of treatment-emergent adverse events. Patients were evaluated at baseline, at weeks 1, 5, 9, 13, and 17. A total of 779 patients (595 women [76.4%] with a mean [SD] age of 50.2 [13.65] and 184 men [23.6%] with a mean [SD] age of 52.4 [14.6] years) were enrolled into the study; 687 (88.2%) patients completed the study. The mean (SD) daily dose of mirtazapine orally disintegrating tablets was 29.0 (3.8) mg. The mean total (SD) HAMD-17 score improved significantly from 25.7 (4.6) to 7.3 (4.3) (P<0.005). At each visit, the mean HAMD-17 score was significantly lower than that at the preceding visit. At week 17, remission (HAMD-17 score < or =7) was observed in 436 (56%) patients. The mean (SD) CGI-S score improved significantly from 4.9 (1.0) at baseline to 1.5 (0.6) at endpoint (P<0.001). According to the CGI-I assessments, 621 patients (89.4%) improved and improved very much. The vast majority of patients (80%) preferred the new formulation of mirtazapine – mirtazapine orally disintegrating tablet. Treatment-emergent adverse events occurred in 106 patients (13.6%). The most frequent adverse events were weight gain, sedation, dizziness, and dry mouth. In this study conducted in Lithuania with depressed patients, a significant improvement was shown in all efficacy measures. In addition, mirtazapine orally disintegrating tablet was a well-tolerated and preferable formulation for the treatment of depressed patients.
The value of transcutaneous method of bilirubin measurement in newborn population with the risk of ABO hemolytic disease
Authors: Stonienė Dalia , Buinauskienė Jūratė , Markūnienė Eglė
Published: June 2018

Objective of the study. To evaluate the correlation between total serum bilirubin (TSB) and transcutaneous bilirubin (TcB) levels in newborn infants at risk of ABO hemolytic disease. Material and methods. During a prospective study, 130 full-term (≥37 weeks of gestation) newborn infants with diagnosed ABO blood group incompatibility were examined. TSB level was measured at the age of 6 hours; further measurements were performed at 24, 48, and 72 hours following the first measurement. Blood samples were collected from the peripheral veins. In clinical laboratory, total serum bilirubin level was measured using Jendrassik-Grof method. TcB level in the forehead was measured using a noninvasive bilirubinometer BiliCheck (SpectRX Inc, Norcross, GA) according to the manufacturer’s instructions within ±30 min after getting a blood sample. Results. During the study, 387 double tests were performed to measure TSB and TcB levels. TSB level (114.83 [62.85] μmol/L) closely correlated with TcB level (111.51 [61.31] μmol/L) (r=0.92, P<0.001). The strongest correlation was reported at the age of 54 hours (r=0.873, P<0.001), the weakest – at the age of 6 hours (r=0.729, P<0.001). TSB and TcB levels showed a strong correlation; the difference between these values was significant (95% CI, 0.70; 5.93; P<0.05). The greatest difference between TSB and TcB levels was detected at the age of 6 hours (5.58 [17.46] μmol/L, 95% CI, 2.55; 8.61; P<0.001). No significant difference was reported at the age of 30, 54, and 78 hours. Using linear regression analysis, it was established that correlation of TSB and TcB was described by equation y=14.13+0.903x. Transcutaneously measured bilirubin level underestimated serum bilirubin level. When at the age of 6 hours TcB level is ≥98 μmol/L, ABO hemolytic disease in newborns may be diagnosed with 100% sensitivity and 98% specificity; positive predictive value was 62% and negative predictive value was 100%. While a newborn’s age increases, TcB sensitivity and specificity for diagnosing ABO hemolytic disease decrease. Conclusion. While evaluating bilirubin level transcutaneously according to nomograms of serum bilirubin level, the results should be considered with caution, especially for newborns with a risk of ABO hemolytic disease. The hour-specific nomograms of transcutaneous
Trends of smoking prevalence among Lithuanian school-aged children in 1994–2006
Authors: Zaborskis Apolinaras , Šumskas Linas , Žemaitienė Nida , Grabauskas Vilius , Veryga Aurelijus , Petkevičius Robertas
Published: June 2018

Background. Despite much effort spent on antismoking programs in schools in different countries, limited effects have been observed in many cases. Evidence from European countries shows that active tobacco control actions such as ban on tobacco advertising, increase of tobacco taxes could lead to successful results. Our study was aimed to analyze time trends on smoking in Lithuanian school-aged children during the period of 1994–2006 in the context of antismoking policies, which were implemented in Lithuania. Material and methods. This study was a part of WHO Cross-National Health Behavior in School-Aged Children (HBSC) study carried out in Lithuania. The standardized methods of international HBSC study protocol were applied. Stratified random representative samples of 5428, 4513, 5645, and 5632 students aged 11, 13, and 15 years were included into schoolbased anonymous questionnaire surveys in 1994, 1998, 2002, and 2006, respectively (spring semester). Questions on frequency of smoking, age of initiation and other questions were included. Response rates of each of these four questionnaire surveys were higher than 90%. Results. Smoking behavior was more common among boys. The prevalence gap in smoking between boys and girls diminished during period of observation. Prevalence of smoking increased significantly among boys during the period of 1994–2002 (11.3%, 19.8%, and 23.6% in 1994, 1998, and 2002, respectively), but started to decline after (17.3% in 2006, P<0.05). Similar trends were observed among girls: 3.6%, 8.5%, 14.6%, and 12.5% of girls reported smoking in cross-sectional surveys of 1994, 1998, 2002, and 2006, respectively. Boys living in rural areas were more frequent smokers than those living in urban areas in 1994–1998 (9.5% vs 13.9%, P<0.05). However, the surveys of 2002–2006 showed opposite changes (25.6% vs 22.1%, P<0.05 and 17.8% vs 16.9%, P>0.05). Urban girls have reported smoking more frequently in comparison with rural girls. Conclusions. An increase in tobacco smoking among school-aged children was observed in Lithuania during 1994–2002. A decrease in prevalence of smoking was seen during the period of 2002–2006. These trends could be related to the implementation of tobacco control measures in Lithuania.
The need for orthodontic treatment among 10–11- and 14–15-year-old Lithuanian schoolchildren
Authors: Baubinienė Diana , Šidlauskas Antanas , Misevičienė Irena
Published: June 2018

The aim of this study was to evaluate the need for orthodontic treatment among 10–11- and 14–15-year-old schoolchildren in Lithuania. Material and methods. A total of 4235 children randomly selected from different socioeconomic backgrounds were examined. The schoolchildren were divided into two age groups: first group, 10–11-year olds (1142 boys, 1180 girls) and second group, 14–15-year olds (936 boys, 977 girls). The normative orthodontic treatment need was assessed using the Index of Complexity, Outcome, and Need. Results. The need for orthodontic treatment ranged from 37.4 to 48.9% in 10 counties of Lithuania. The study demonstrated that the need of orthodontic treatment significantly depended on age and to some extent on gender of the schoolchildren examined. This study has shown reduction in the need for orthodontic treatment from 49.9% in the late mixed dentition stage to 33.9% in the permanent dentition stage. Conclusions. The need for orthodontic treatment is high in Lithuania: almost half of 10–11- year-old and every third of 14–15-year-old schoolchildren need orthodontic treatment.
Characterization of Cotton Ball-like Au/ZnO Photocatalyst Synthesized in a Micro-Reactor
Authors: Kim Ki-Joong , Kreider Peter B. , Ahn Ho-Geun , Chang Chih-Hung
Published: June 2018

Noble metal/metal oxide nanostructures are an efficient system in photocatalysis. Continuous and scalable production of advanced particle systems will be a requirement for commercial-scale deployment for many applications, including photocatalysis. In this work, Au/ZnO structures were synthesized in a continuous flow micro-reactor at room temperature and the detailed characteristics of the product indicate a specific cotton ball-like core-shell microstructure that showcases specific advantages compared to traditional batch synthesis methods. The formation pathway of the core-shell Au/ZnO structures is discussed with the pH-dependent speciation diagram, and photocatalytic activity was assessed under simulated sunlight, demonstrating the enhanced performance of the cotton ball-like Au/ZnO microstructures in photocatalytic dye degradation. This work describes the application of microreaction technology in the continuous production of metal/metal oxide photocatalysts.
The Evolution of Integrated Interfaces for MEMS Microphones
Authors: Malcovati Piero , Baschirotto Andrea
Published: June 2018

Over the last decade, MEMS microphones have become the leading solution for implementing the audio module in most portable devices. One of the main drivers for the success of the MEMS microphone has been the continuous improvement of the corresponding integrated interface circuit performance in terms of both dynamic range and power consumption, which enabled the introduction in mobile devices of additional functionalities, such as Hi-Fi audio recording or voice commands. As a result, MEMS microphone interface circuits evolved from just simple amplification stages to complex mixed-signal circuits, including A/D converters, with ever improving performance. This paper provides an overview of such evolution based on actual design examples, focusing, finally, on the latest cutting-edge solutions.
Properties and Stability of Perilla Seed Protein-Stabilized Oil-in-Water Emulsions: Influence of Protein Concentration, pH, NaCl Concentration and Thermal Treatment
Authors: Liu Ning , Chen Qiannan , Li Guanghui , Zhu Zhenbao , Yi Jianhua , Li Cheng , Chen Xuefeng , Wang Yong
Published: June 2018

Perilla seed protein (PSP) was extracted from defatted perilla seed meal and applied in oil-in-water (O/W) emulsions as an emulsifier. We investigated the influences of protein concentration (0.25–1.5 wt %), pH (3.0–9.0), NaCl concentration (0–350 mmol/L) and thermal treatment (70–90 °C, 30 min) on the physical characteristics of O/W emulsions, including volume-average diameter, ζ-potential, interfacial protein concentration, microstructure and so on. Results showed that increasing PSP concentration would decrease the d4,3 and a 1.0 wt % PSP concentration was sufficient to ensure the stability of emulsion. Under pH 3.0–9.0, emulsions were stable except at pH 3.0–5.0 which was proximal to the isoelectric point (pH 4.5) of PSP. At high NaCl concentrations (250–350 mmol/L), the emulsions exhibited relatively lower absolute ζ-potential values and a large number of aggregated droplets. A moderate thermal treatment temperature (e.g., 70 °C) was favorable for the emulsion against aggregation and creaming. However, when 90 °C thermal treatment was performed, a clear layer separation was observed after 2 weeks storage and the emulsion showed a poor stability. The findings of this work are of great importance for the utilization and development of PSP as an emulsifier for food emulsions.
Whole-Genome Comparison Reveals Divergent IR Borders and Mutation Hotspots in Chloroplast Genomes of Herbaceous Bamboos (Bambusoideae: Olyreae)
Authors: Wang Wencai , Chen Siyun , Zhang Xianzhi
Published: June 2018

Herbaceous bamboos (Olyreae) are a separate lineage with idiosyncratic traits, e.g., unisexual flowers and annual or seasonal flowering lifestyle, in the grass family. To elucidate the evolution of herbaceous bamboos we produced two complete chloroplast (cp) genomes from two monotypic genera i.e., Froesiochloa and Rehia via the genome-skimming approach. The assembled F. boutelouoides and R. nervata cp genomes were 135,905 and 136,700 base-pair (bp), respectively. Further whole-genome comparative analyses revealed that the cp genes order was perfectly collinear, but the inverted repeats (IRs) borders, i.e., the junctions between IRs and single copy regions, were highly divergent in Olyreae. The IRs expansions/contractions occurred frequently in Olyreae, which have caused gene content and genome size variations, e.g., the copy number reduction of rps19 and trnH(GUG) genes in Froesiochloa. Subsequent nucleotide mutation analyses uncovered a greatly heterogeneous divergence pattern among different cpDNA regions in Olyreae cp genomes. On average, non-coding loci evolved at a rate of circa 1.9 times faster than coding loci, from which 20 rapidly evolving loci were determined as potential genetic markers for further studies on Olyreae. In addition, the phylogenomic analyses from 67 grass plastomes strongly supported the phylogenetic positions of Froesiochloa and Rehia in the Olyreae.
Cholesterol-Lowering and Liver-Protective Effects of Cooked and Germinated Mung Beans (Vigna radiata L.)
Authors: Lopes Lays Arnaud Rosal , Martins Maria do Carmo de Carvalho e , Farias Luciana Melo de , Brito Ana Karolinne da Silva , Lima Geovanni de Morais , Carvalho Vanessa Brito Lira de , Pereira Cristian Francisco de Carvalho , Conde Júnior Aírton Mendes , Saldanha Tatiana , Arêas José Alfredo Gomes , Silva Kaesel Jackson Damasceno e , Frota Karoline de Macêdo Gonçalves
Published: June 2018

We investigated the hypocholesterolemic and liver-protective effects of cooked and germinated whole mung beans. Hamsters were fed for 28 days on diets rich in saturated fatty acids and cholesterol, differing only in protein source (20%): casein, cooked whole mung bean, and germinated mung bean. After 28 days, we found reduced plasma concentrations of total cholesterol and non-HDL cholesterol, increased faecal cholesterol excretion, and reduced levels of asparagine aminotransferase and alanine aminotransferase enzymes in the liver. Reduction in hepatic lipid deposition was observed between each of the mung bean groups relative to the casein group. In addition, the animals of the geminated mung bean group showed a lack of inflammatory infiltrate and better vascularisation of the hepatic tissue. Results from this study show significant hypocholesterolemic and liver-protective properties of the mung bean, which are further enhanced after germination.
Effects of Synbiotics among Constipated Adults in Serdang, Selangor, Malaysia—A Randomised, Double-Blind, Placebo-Controlled Trial
Authors: Lim Ying Jye , Jamaluddin Rosita , Hazizi Abu Saad , Chieng Jin Yu
Published: June 2018

Synbiotics approach complementarily and synergistically toward the balance of gastrointestinal microbiota and improvement in bowel functions. A randomised, double-blind, placebo-controlled study was conducted to examine the effects of a synbiotics supplement among constipated adults. A total of 85 constipated adults, diagnosed by Rome III criteria for functional constipation were randomised to receive either synbiotics (n = 43) or placebo (n = 42) once daily (2.5 g) in the morning for 12 weeks. Eight times of follow-up was conducted every fortnightly with treatment response based on a questionnaire that included a record of evacuation (stool frequency, stool type according to Bristol Stool Form Scale), Patients Assessment on Constipation Symptoms (PAC-SYM), and Patients Assessment on Constipation Quality of Life (PAC-QOL). There were no significant differences in stool evacuation, but defecation frequency and stool type in treatment group were improved tremendously than in placebo group. While the treatment group was reported to have higher reduction in severity of functional constipation symptoms, the differences were not statistically significant. Dietary supplementation of synbiotics in this study suggested that the combination of probiotics and prebiotics improved the functional constipation symptoms and quality of life although not significant. This was due to the high placebo effect which synbiotics failed to demonstrate benefit over the controls.
Beverage Consumption Patterns among Infants and Young Children (0–47.9 Months): Data from the Feeding Infants and Toddlers Study, 2016
Authors: Kay Melissa C. , Welker Emily B. , Jacquier Emma F. , Story Mary T.
Published: June 2018

(1) Background: Data about early life beverage intake patterns is sparse. We describe beverage patterns among infants and young children from the Feeding Infants and Toddlers Study (FITS) 2016. (2) Methods: FITS 2016 is a cross-sectional survey of U.S. parents/caregivers of children 0–47.9 months (n = 3235). Food and beverage intakes were collected by 24-h dietary recalls to describe beverage consumption patterns including: a) prevalence of consumption, per capita and per consumer intake, b) contribution to intake of calories and key nutrients, and c) prevalence according to eating occasions. (3) Results: Breast milk and infant formula were commonly consumed among <12-month-olds. Among 12–23.9-month-olds, the most commonly consumed beverage was whole milk (67% consuming), followed by 100% juice (50% consuming). Plain drinking water was consumed by 70% of 12–23.9-month-olds and 78% of 24–47.9-month-olds. Among 12–47.9-month-olds, milks provided more energy and key nutrients than all other beverages. Across eating occasions, sugar-sweetened beverage (SSB) consumption, especially in the form of fruit-flavored drinks, was higher among 24–47.9 compared to 12–23.9-month-olds. Only 23–32% of ≥12-month-olds consumed milk or water at lunch or dinner. (4) Conclusions: Opportunities exist to improve beverage patterns. Future interventions may benefit from focusing on timely introduction of age-appropriate beverages and reducing consumption of SSBs.
Oral Intake of Low-Molecular-Weight Collagen Peptide Improves Hydration, Elasticity, and Wrinkling in Human Skin: A Randomized, Double-Blind, Placebo-Controlled Study
Authors: Kim Do-Un , Chung Hee-Chul , Choi Jia , Sakai Yasuo , Lee Boo-Yong
Published: June 2018

Collagen-peptide supplementation could be an effective remedy to improve hydration, elasticity, and wrinkling in human skin. The aim of this study was to conduct a double-blind, randomized, placebo-controlled trial to clinically evaluate the effect on human skin hydration, wrinkling, and elasticity of Low-molecular-weight Collagen peptide (LMWCP) with a tripetide (Gly-X-Y) content >15% including 3% Gly-Pro-Hyp. Individuals (n = 64) were randomly assigned to receive either placebo or 1000 mg of LMWCP once daily for 12 weeks. Parameters of skin hydration, wrinkling, and elasticity were assessed at baseline and after 6 weeks and 12 weeks. Compared with the placebo group, skin-hydration values were significantly higher in the LMWCP group after 6 weeks and 12 weeks. After 12 weeks in the LMWCP group, visual assessment score and three parameters of skin wrinkling were significantly improved compared with the placebo group. In case of skin elasticity, one parameter out of three was significantly improved in the LMWCP group from the baseline after 12 weeks, while, compared with the placebo group, two parameters out of three in the LMWCP group were higher with significance after 12 weeks. In terms of the safety of LMWCP, none of the subjects presented adverse symptoms related to the test material during the study period. These results suggest that LMWCP can be used as a health functional food ingredient to improve human skin hydration, elasticity, and wrinkling.
Dietary Patterns in Secondary Prevention of Heart Failure: A Systematic Review
Authors: Dos Reis Padilha Gabriela , Sanches Machado d’Almeida Karina , Ronchi Spillere Stefanny , Corrêa Souza Gabriela
Published: June 2018

Background: Diet is an important factor in secondary prevention of heart failure (HF) but there is still no consensus as to which dietary model should be adopted by this population. This systematic review aims to clarify the relationship between dietary patterns and secondary prevention in HF. Methods: We searched the Medline, Embase and Cochrane databases for studies with different dietary patterns and outcomes of secondary prevention in HF. No limitation was used in the search. Results: 1119 articles were identified, 12 met the inclusion criteria. Studies with Dietary Approaches to Stop Hypertension (DASH), Mediterranean, Hyperproteic and Low-carb diets were found. The DASH pattern showed improvement in cardiac function, functional capacity, blood pressure, oxidative stress and mortality. The Mediterranean diet had a correlation with inflammation, quality of life and cardiac function but just on cross-sectional studies. Regarding the Hyperproteic and Low-carb diets only one study was found with each pattern and both were able to improve functional capacity in patients with HF. Conclusions: DASH pattern may have benefits in the secondary prevention of HF. The Mediterranean diet demonstrated positive correlation with factors of secondary prevention of HF but need more RCTs and cohort studies to confirm these effects. In addition, the Hyperproteic and Low-carb diets, despite the lack of studies, also demonstrated positive effects on the functional capacity in patients with HF.
Effect of Lipid Additives and Drug on the Rheological Properties of Molten Paraffin Wax, Degree of Surface Drug Coating, and Drug Release in Spray-Congealed Microparticles
Authors: Ouyang Hongyi , Zheng Audrey Yi , Heng Paul Wan Sia , Chan Lai Wah
Published: June 2018

Paraffin wax is potentially useful for producing spray-congealed drug-loaded microparticles with sustained-release and taste-masking properties. To date, there is little information about the effects of blending lipids with paraffin wax on the melt viscosity. In addition, drug particles may not be entirely coated by the paraffin wax matrix. In this study, drug-loaded paraffin wax microparticles were produced by spray-congealing, and the effects of lipid additives on the microparticle production were investigated. The influence of lipid additives (stearic acid, cetyl alcohol, or cetyl esters) and drug (paracetamol) on the rheological properties of paraffin wax were elucidated. Fourier transform-infrared spectroscopy was conducted to investigate the interactions between the blend constituents. Selected formulations were spray-congealed, and the microparticles produced were characterized for their size, drug content, degree of surface drug coating, and drug release. The viscosity of wax-lipid blends was found to be mostly lower than the weighted viscosity when interactions occurred between the blend constituents. Molten paraffin wax exhibited Newtonian flow, which was transformed to plastic flow by paracetamol and pseudoplastic flow by the lipid additive. The viscosity was decreased with lipid added. Compared to plain wax, wax-lipid blends produced smaller spray-congealed microparticles. Drug content remained high. Degree of surface drug coating and drug release were also higher. The lipid additives altered the rheological properties and hydrophobicity of the melt and are useful for modifying the microparticle properties.
Pharmacy Practice and Education in Austria
Authors: Langer Thierry , Spreitzer Helmut , Ditfurth Teresa , Stemer Gunar , Atkinson Jeffrey
Published: June 2018

The PHARMINE (“Pharmacy Education in Europe”) project studied pharmacy practice and education in the European Union (EU) member states. The work was carried out using an electronic survey sent to chosen pharmacy representatives. The surveys of the individual member states are now being published as reference documents for students and staff interested in research on pharmacy education in the EU and in mobility. This paper presents the results of the PHARMINE survey on pharmacy practice and education in Austria. In the light of this, we examine the harmonisation of practice and education in Austria with EU norms.