bluuebunny/crossref_metadata_2025 huggingface dataset:
Chemical structures, biological activities, and medicinal potentials of amine compounds detected from Aloe species
Authors: Yadeta Adamu Tizazu
Published: March 2024

Unrestricted interest in Aloe species has grown rapidly, and a lot of research is currently being done to learn more about the properties of the various Aloe constituents. Organic compounds containing amine as functional group are present in a vivid variety of compounds, namely, amino acids, hormones, neurotransmitters, DNA, alkaloids, dyes, etc. These compounds have amine functional groups that have various biological activities, which make them responsible for medicinal potential in the form of pharmaceutical, nutraceutical, and cosmeceutical applications. Consequently, the present review work provides an indication of the amines investigated in Aloe species and their therapeutic uses. Various amine compounds of the Aloe species have effective biological properties to treat diseases. Generally, the genus Aloe has various active amine-containing compounds to combat diseases when humans use them in various forms.
Retrieval-based inference in the acquired equivalence paradigm
Authors: Houser Troy M. , Krantz Louisa , Zeithamova Dagmar
Published: March 2024

Introduction

Generalization is fundamental to cognition. In acquired equivalence, two stimuli that share a common association become treated as equivalent, with information acquired about one stimulus generalizing to the other. Acquired equivalence has been thought to rely on integrating related memories as they are encoded, resulting in fast spontaneous generalization, but other studies suggested effortful on-demand recombination of initially separate memories at retrieval. Here, we tested whether the tendency to separate vs. integrate related information may depend on a methodological detail of a traditional acquired equivalence paradigm.

Methods

Human participants underwent feedback-based learning of overlapping face-scene associations, choosing a correct scene for a face from two options on each trial. Foil (incorrect) scenes were controlled for half of the participants to ensure that they can only learn from corrective feedback. The other half had foils selected randomly on each trial, allowing statistical learning of face-scene co-occurrence to supplement feedback-based learning. We hypothesized that the opportunity for statistical learning would boost learning and generalization and facilitate memory integration.

Results

The opportunity for statistical learning increased associative learning and generalization. However, rather than integrated memories, generalization was increased through learning during test.

Discussion

The results indicate that the tendency for generalization in the acquired equivalence is rather small, with no evidence for integrative encoding irrespective of group. The results inform current debates regarding encoding-based vs. retrieval-based mechanisms of generalization. They also highlight how methodological details may alter performance and the involvement of cognitive processes that underlie it.
Seizing the moment: communicating ethics, decisions, and neurotechnological approaches to pediatric drug-resistant epilepsy
Authors: Illes Judy , McDonald Patrick J. , Ibrahim George M. , Connolly Mary B. , Naftel Robert P. , Bacani Marianne , Nuechterlein Anna , Go Samantha P. , Roduit Johann
Published: March 2024

It is a fundamental duty of neuroscientists to discuss the results of research and related ethical implications. Engagement with neuroscience is especially critical for families with children affected by disorders such as drug resistant epilepsy (DRE) as they navigate complex decisions about innovations in treatment that increasingly include invasive neurotechnologies. Through an evidence-based, iterative, and value-guided approach, we created the short-form documentary film, Seizing Hope: High Tech Journeys in Pediatric Epilepsy, to delve into the relationship between experts with first-hand, lived experience – youth with DRE and caregivers – and physician experts as they weigh medical and ethical trade-offs on this landscape. We describe the co-creation and evolution of this film, screenings, and feedback. Survey responses from 385 viewers highlight new developments in technologies for the treatment of DRE, how families navigate choices for treatment with brain technology, and a sense of hope for the future for children with epilepsy as key attributes of this science communication piece.
Long live the Liver King: right-wing carnivorism and the digital dissemination of primal rhetoric
Authors: Muller S. Marek , Rooney David , Cerja Cecilia
Published: March 2024

In this study, we link pertinent concepts from rhetorical studies of the alt-right, animality studies, and conspiracism to explicate the production and dissemination of “carnivore diets” through digital social networks. Taking the ideological and discursive interconnections of animality, hegemonic masculinity, and white nationalism seriously, we conceptualize primal rhetorical networks: a web of influencers and agitators who espouse an ahistorical, carnivorous primal rhetoric concerned with Modern Man's physical and spiritual downfall and his potential for resurrection by consuming nonhuman animals. We utilize a corpus of online texts and social media posts between 2020 and 2023 to perform an ideological rhetorical criticism of two interconnected, online Carnivore Diet gurus: the Liver King and the Raw Egg Nationalist. Drawing upon “conspiritual” tropes and raced/gendered depictions of nonhuman animal consumption, we demonstrate how these networked rhetors “sell” their lifestyles to make a profit and “convert” their followers into an ever-growing network of white nationalist, cisheterosexist, carnivorous ideologues.
Leveraging small island context to advance and disseminate environmental health and sustainable development knowledge through higher education
Authors: Telesford Lindonne , Nsobundu Chinelo , Lewis Tiffany , Marks Aleah , Alamrany Amran , Zaim Oznur , Lachica Innah , Eruaga Allen , Roman Lira Camille , Slavkovska Tamara , Mandal Dhruv , Chandran Vijay
Published: March 2024

Higher education curricula can be designed to effectively leverage the context of small island states to enhance and deliver sustainable development and environmental health education, catering to workforce development, citizenry, and inclusivity. Small islands and developing states, in particular, are left behind in recognizing and promoting the linkages between environmentally sustainable development and the health of its people. This community case study highlights strategies of a Caribbean-based higher learning institution, leveraging the context of a small island state, to close this gap and create equitable access to integrated sustainable development and environmental health education. Tenets of Education for Sustainable Development theory underpin the pedagogical methodologies used in the delivery of the academic program. The curriculum draws on experiential learning, competency-based education, and diversity and inclusion, oriented to active student–student and student-practitioner engagements. Learning is facilitated through the effective dissemination of information, healthy interactions within a diverse community, professional engagements with multi-disciplinary practitioners, problem-solving, and formulation and presentation of perspectives and experiences relating to the Sustainable Development Agenda and Goals. Highly engaging and inclusive academic processes can accentuate students transitioning into the role of teachers, environmentally conscious leaders and citizens, and competent public health practitioners for the local and global workforces.
Causal relationship of genetically predicted gut microbiota with thyroid cancer: a bidirectional two-sample mendelian randomization study
Authors: Sun Xiaohe , Chen Shuai , Zhao Shuoqi , Wang Jingwen , Cheng Haibo
Published: March 2024

Background

Previous investigations have demonstrated a correlation between the composition of gut microbiota and the development of thyroid cancer (TC). Nonetheless, there was no consensus on the causal effect of gut microbiota composition on TC risk. Therefore, the present study aimed to perform a bidirectional two-sample Mendelian randomization (MR) analysis to explore potential causal associations between gut microbiota and TC risk.

Methods

Utilizing data from the MiBioGen consortium’s genome-wide association studies (GWAS) meta-analysis involving a sample size of 18,340, we identified instrumental variables for 211 gut microbiota taxa. The summary statistics for TC was from relevant large-scale GWAS conducted by the FinnGen consortium. In the first stage, the Inverse-variance weighted (IVW) method was used as the primary estimate method, and the stability of estimations was tested by a battery of sensitivity analyses. In the second stage, a reverse MR analysis was applied to determine whether reverse causality existed.

Results

According to the IVW method, we identified 9 genetically predicted gut microbiota that were causally correlated with TC risk. Among them, we observed a positive causal effect of Family Christensenellaceae (OR = 1.664, 95% CI: 1.103–2.511, P = 0.015), Family Victivallaceae (OR = 1.268, 95% CI: 1.009–1.594, P = 0.042), Genus Methanobrevibacter (OR = 1.505, 95% CI: 1.049–2.159, P = 0.027), Genus Ruminococcus2 (OR = 1.846, 95% CI: 1.261–2.704, P = 0.002), Genus Subdoligranulum (OR = 1.907, 95% CI: 1.165–3.121, P = 0.010), Phylum Verrucomicrobia (OR = 1.309, 95% CI: 1.027–1.668, P = 0.029) on TC risk, while Class Betaproteobacteria (OR = 0.522, 95% CI: 0.310–0.879, P = 0.015), Family Family XI (OR = 0.753, 95% CI: 0.577–0.983, P = 0.037), Genus Sutterella (OR = 0.596, 95% CI: 0.381–0.933, P = 0.024) might be correlated with a decreased risk of TC. Subsequently, various sensitivity analyses indicated no heterogeneity, directional pleiotropy or outliers. In addition, reverse analysis demonstrated a negative causal effect of TC risk on the abundance of the gut microbiota (Genus Ruminococcus2, OR = 0.947, 95% CI: 0.907–0.989, P = 0.014).

Conclusion

Genetic evidence suggested that bidirectional causal associations of specific bacteria taxa and the risk of TC, highlighting the association of the “gut-thyroid” axis. Further exploration of the potential microbiota-related mechanisms might have profound implications for public health in terms of the early prevention and treatment of TC.
Unveiling peripheral neuropathy and cognitive dysfunction in diabetes: an observational and proof-of-concept study with video games and sensor-equipped insoles
Authors: Ming Antao , Lorek Elisabeth , Wall Janina , Schubert Tanja , Ebert Nils , Galatzky Imke , Baum Anne-Katrin , Glanz Wenzel , Stober Sebastian , Mertens Peter R.
Published: March 2024

Background

Proactive screening for cognitive dysfunction (CD) and peripheral neuropathy (PNP) in elderly patients with diabetes mellitus is essential for early intervention, yet clinical examination is time-consuming and prone to bias.

Objective

We aimed to investigate PNP and CD in a diabetes cohort and explore the possibility of identifying key features linked with the respective conditions by machine learning algorithms applied to data sets obtained in playful games controlled by sensor-equipped insoles.

Methods

In a cohort of patients diagnosed with diabetes (n=261) aged over 50 years PNP and CD were diagnosed based on complete physical examination (neuropathy symptom and disability scores, and Montreal Cognitive Assessment). In an observational and proof-of-concept study patients performed a 15 min lasting gaming session encompassing tutorials and four video games with 5,244 predefined features. The steering of video games was solely achieved by modulating plantar pressure values, which were measured by sensor-equipped insoles in real-time. Data sets were used to identify key features indicating game performance with correlation regarding CD and PNP findings. Thereby, machine learning models (e.g. gradient boosting and lasso and elastic-net regularized generalized linear models) were set up to distinguish patients in the different groups.

Results

PNP was diagnosed in 59% (n=153), CD in 34% (n=89) of participants, and 23% (n=61) suffered from both conditions. Multivariable regression analyses suggested that PNP was positively associated with CD in patients with diabetes (adjusted odds ratio = 1.95; 95% confidence interval: 1.03-3.76; P=0.04). Predictive game features were identified that significantly correlated with CD (n=59), PNP (n=40), or both (n=59). These features allowed to set up classification models that were enriched by individual risk profiles (i.e. gender, age, weight, BMI, diabetes type, and diabetes duration). The obtained models yielded good predictive performance with the area under the receiver-operating-characteristic curves reaching 0.95 for CD without PNP, 0.83 for PNP without CD, and 0.84 for CD and PNP combined.

Conclusions

The video game-based assessment was able to categorize patients with CD and/or PNP with high accuracy. Future studies with larger cohorts are needed to validate these results and potentially enhance the discriminative power of video games.
Differences in the clinical and hormonal presentation of patients with familial and sporadic primary aldosteronism
Authors: Araujo-Castro Marta , Parra Paola , Martín Rojas-Marcos Patricia , Paja Fano Miguel , González Boillos Marga , Pascual-Corrales Eider , García Cano Ana María , Ruiz-Sanchez Jorge Gabriel , Vicente Delgado Almudena , Gómez Hoyos Emilia , Ferreira Rui , García Sanz Iñigo , Recasens Sala Mònica , Barahona San Millan Rebeca , Picón César María José , Díaz Guardiola Patricia , Perdomo Carolina M. , Manjón-Miguélez Laura , García Centeno Rogelio , Rebollo Román Ángel , Gracia Gimeno Paola , Robles Lázaro Cristina , Morales-Ruiz Manuel , Calatayud María , Furio Collao Simone Andree , Meneses Diego , Sampedro Nuñez Miguel , Escudero Quesada Verónica , Mena Ribas Elena , Sanmartín Sánchez Alicia , Gonzalvo Diaz Cesar , Lamas Cristina , del Castillo Tous María , Serrano Gotarredona Joaquín , Michalopoulou Alevras Theodora , Moya Mateo Eva María , Hanzu Felicia A.
Published: March 2024

Purpose

To compare the clinical and hormonal characteristics of patients with familial hyperaldosteronism (FH) and sporadic primary aldosteronism (PA).

Methods

A systematic review of the literature was performed for the identification of FH patients. The SPAIN-ALDO registry cohort of patients with no suspicion of FH was chosen as the comparator group (sporadic group).

Results

A total of 360 FH (246 FH type I, 73 type II, 29 type III, and 12 type IV) cases and 830 sporadic PA patients were included. Patients with FH-I were younger than sporadic cases, and women were more commonly affected (P = 0.003). In addition, the plasma aldosterone concentration (PAC) was lower, plasma renin activity (PRA) higher, and hypokalemia (P < 0.001) less frequent than in sporadic cases. Except for a younger age (P < 0.001) and higher diastolic blood pressure (P = 0.006), the clinical and hormonal profiles of FH-II and sporadic cases were similar. FH-III had a distinct phenotype, with higher PAC and higher frequency of hypokalemia (P < 0.001), and presented 45 years before sporadic cases. Nevertheless, the clinical and hormonal phenotypes of FH-IV and sporadic cases were similar, with the former being younger and having lower serum potassium levels.

Conclusion

In addition to being younger and having a family history of PA, FH-I and III share other typical characteristics. In this regard, FH-I is characterized by a low prevalence of hypokalemia and FH-III by a severe aldosterone excess causing hypokalemia in more than 85% of patients. The clinical and hormonal phenotype of type II and IV is similar to the sporadic cases.
Case studies of three geological archives for rare radionuclide measurements using accelerator mass spectrometry
Authors: Fichter Sebastian , Koll Dominik , Rolofs Annabel , Wallner Anton
Published: March 2024

Long-lived radionuclides in our environment provide important information on natural and anthropogenic processes. Their presence and concentration reflect the balance of production and decay. Geological archives store such information and the nuclides can be chemically extracted from the bulk sample. Accelerator mass spectrometry (AMS) represents a sensitive method to quantify those nuclides at natural levels. Three different terrestrial archives are discussed here as examples for radionuclide extraction using various chemical separation methods for subsequent AMS measurements. We focus on sample preparation for the cosmogenic radionuclides 10Be and 26Al, various anthropogenic actinide isotopes such as U, Pu, and Am as well as the astrophysically interesting nuclides 41Ca, 53Mn, and 60Fe. The processed materials cover samples with masses between a few mg and up to a few hundred kg and protocols are presented for the quantitative extraction of some 10,000 atoms of cosmogenic or interstellar origin per sample and even as low as a few hundred actinide atoms.
Integrating monetary and non-monetary valuation for ecosystem services in Piatra Craiului national park, Southern Carpathians: a comprehensive approach to sustainability and conservation
Authors: Chivulescu Serban , Hapa Mihai , Pitar Diana , Lorenţ Adrian , Marmureanu Luminita , Leca Stefan , Radu Raul , Cazacu Roxana , Dobre Alexandru Claudiu , Pascu Ionut Silviu , Marcu Cristiana , Verghelet Mircea , Vezeanu Constantin , Racoviceanu Tudor , Badea Ovidiu
Published: March 2024

The concept of ecosystem services and their valuation has gained significant attention in recent years due to the profound interdependence and interconnectedness between humans and ecosystems. As several studies on valuation of forest ecosystem services have stressed the human-nature interactions lately, in the research study area, the environmental conditions shows rapid changes while human pressures on forests intensify. Thus, the research questions are as follows: (i) what are the the monetary and non-monetary value of ecosystem services provided by forests in Piatra Craiului National Park and (ii) their relationship with other variables, focusing on identifying differences and resemblances between each approach. The R PASTECS package was utilized to analyze primary statistical indicators for both monetary and non-monetary values, revealing significant variability in the results (s% monetary 141% and s% non-monetary 62%). Both monetary and non-monetary assessments were computed at the management unit level and the data used was provided by the Forest Management plans and photograph analysis which have significant value as indicators of ecosystem services. The correlation between nature and culture was assessed through social-media based method, highly known to stimulate participant engagement while the quantitative data was assessed through forest data computation and PCA method for visualization. The research highlighted that, in monetary terms, the minimum value of identified ecosystem services was €34 and the maximum value exceeded €570,000 at management unit level and in non-monetary terms, the values ranged from 1 to 5 (kernel score). The research reveals a substantial variability in both types of valuations. Strong associations between certain variables (monetary value with carbon stock and stand volume), moderate connections (slope with stand productivity), and weaker relationships (non-monetary value with altitude, age with slope, type of flora with altitude, and altitude with stand productivity) were revealed. The findings provided valuable insights for policymakers, land managers, and stakeholders involved in natural resource management and conservation, emphasizing the importance of considering both economic and non-economic benefits in decision-making processes. The integrated approach of this study shows how we can better assess the mixed value of ecosystem services, contributing to the ongoing actions of raising awareness and social responsibility.
Genome-wide identification of dysregulated alternative splicing and RNA-binding proteins involved in atopic dermatitis
Authors: Yang Yaqi , Chen Hao , Jiang Qing , Yang Lin , Zhu Rongfei , Huang Nan
Published: March 2024

Objectives: We explored the role and molecular mechanisms of RNA-binding proteins (RBPs) and their regulated alternative splicing events (RASEs) in the pathogenesis of atopic dermatitis (AD).

Methods: We downloaded RNA-seq data (GSE121212) from 10 healthy control skin samples (healthy, Ctrl), 10 non-lesional skin samples with AD damage (non-lesional, NL), and 10 lesional skin samples with AD damage (lesional, LS). We performed the analysis of differentially expressed genes (DEGs), differentially expressed RBPs (DE-RBPs), alternative splicing (AS), functional enrichment, the co-expression of RBPs and RASEs, and quantitative polymerase chain reaction (qPCR).

Results: We identified 60 DE-RBP genes by intersecting 2141 RBP genes from existing reports with overall 2697 DEGs. Most of the DE-RBP genes were found to be upregulated in the AD LS group and related to immune and apoptosis pathways. We observed different ASEs and RASEs among the healthy, AD NL, and AD LS groups. In particular, alt3p and alt5p were the main ASEs and RASEs in AD NL and AD LS groups, compared to the healthy group. Furthermore, we constructed co-expression networks of DE-RBPs and RAS, with particular enrichment in biological pathways including cytoskeleton organization, inflammation, and immunity. Subsequently, we selected seven genes that are commonly present in these three pathways to assess their expression levels in the peripheral blood mononuclear cells (PBMCs) from both healthy individuals and AD patients. The results demonstrated the upregulation of four genes (IFI16, S100A9, PKM, and ENO1) in the PBMCs of AD patients, which is highly consistent with DE-RBP genes analysis. Finally, we selected four RAS genes regulated by RBPs that were related to immune pathways and examined their RASEs in PBMCs from both AD patients and healthy controls. The results revealed an increased percentage of RASEs in the DDX60 gene in AD, which is highly consistent with AS analysis.

Conclusion: Dysregulated RBPs and their associated RASEs may have a significant regulatory role in the development of AD and could be potential therapeutic targets in the future.
Intratumor tertiary lymphatic structure evaluation predicts the prognosis and immunotherapy response of patients with colorectal cancer
Authors: Feng Huijing , Zhang Siyuan , Zhou Qiuru , Han Fei , Du Gang , Wang Lin , Yang Xuena , Zhang Xiying , Yu Wenwen , Wei Feng , Hao Xishan , Ren Xiubao , Zhao Hua
Published: March 2024

Background

Immune checkpoint therapy, involving the programmed cell death 1 (PD-1) monoclonal antibody, has revolutionized the treatment of cancer. Tertiary lymphatic structure (TLS) serves as an immune indicator to predict the efficacy of PD-1 antibody therapy. However, there is no clear result whether the distribution, quantity, and maturity of TLS can be effective indicators for predicting the clinical efficacy of anti-PD1 immunotherapy in patients with colorectal cancer (CRC).

Methods

Fifty-seven patients who underwent surgical resection and thirty-nine patients who received anti-PD-1 immunotherapy were enrolled in this retrospective study. Immunohistochemical staining and multiple fluorescence immunohistochemistry were used to evaluate the mismatch repair (MMR) subtypes and TLS distribution, quantity, and maturity, respectively.

Results

A comprehensive patient score system was built based on TLS quantity and maturity. We found that the proportion of patients with score >1 was much higher in the deficient mismatch repair(dMMR) group than in the proficient mismatch repair(pMMR) group, and this difference was mainly due to intratumoral TLS. Patient score, based on the TLS evaluation of whole tumor, peritumor, or intratumor, was used to evaluate the efficacy of anti-PD1 immunotherapy. Based only on the intratumor TLS evaluation, the proportion of patients with a score >1 was higher in the response (PR + CR) group than in the non-response (PD) group. Multivariate analysis revealed that patient scores were positively correlated with the clinical efficacy of immunotherapy. Further analysis of immune-related progression-free survival was performed in patients with CRC who received anti-PD-1 immunotherapy. Patients with score >1 based on the intratumor TLS evaluation had significantly better survival.

Conclusions

These results suggest that the patient score based on intratumor TLS evaluation may be a good immune predictive indicator for PD-1 antibody therapy in patients with CRC.
Disentangling inhibition and prediction in negation processing
Authors: Haase Viviana
Published: March 2024

Negation as a universal feature of human language is used effortlessly in everyday communication. However, experimental research has shown that the comprehension of negated sentences seems to require additional cognitive resources compared to affirmative sentences. Many studies investigating the processing of negation report longer reading and reaction times for negative compared to affirmative sentences and many studies report a Polarity by Truth interaction: false affirmative sentences lead to longer response times and larger N400 event-related potentials (ERPs) than true affirmative sentences, whereas the pattern is reversed for negative sentences where it is the true sentence that elicits longer reaction times and higher N400 ERPs compared to false negative sentences. These interactions have been discussed in the light of lexical associations, predictability, and the need to construct two subsequent mental representations. Furthermore, recent studies have shown that the comprehension of negated sentences seems to make use of neural resources that are typically involved in cognitive control and inhibitory mechanisms. As both processes have been associated with two different and temporally overlapping ERP components (the N400 and the P300), we focus on studies with high temporal resolution. We discuss linguistic aspects of negation, such as semantic similarity and contextual invariance of negation. We furthermore discuss the role of the verb as well as the position of the negative marker with respect to the verb, and their respective relevance for predictive and inhibitory mechanisms in negated sentences.
Predicting long-term outcomes for acute ischemic stroke using multi-model MRI radiomics and clinical variables
Authors: Wei Lai , Pan Xianpan , Deng Wei , Chen Lei , Xi Qian , Liu Ming , Xu Huali , Liu Jing , Wang Peijun
Published: March 2024

Purpose

The objective of this study was to create and validate a novel prediction model that incorporated both multi-modal radiomics features and multi-clinical features, with the aim of accurately identifying acute ischemic stroke (AIS) patients who faced a higher risk of poor outcomes.

Methods

A cohort of 461 patients diagnosed with AIS from four centers was divided into a training cohort and a validation cohort. Radiomics features were extracted and selected from diffusion-weighted imaging (DWI) and apparent diffusion coefficient (ADC) images to create a radiomic signature. Prediction models were developed using multi-clinical and selected radiomics features from DWI and ADC.

Results

A total of 49 radiomics features were selected from DWI and ADC images by the least absolute shrinkage and selection operator (LASSO). Additionally, 20 variables were collected as multi-clinical features. In terms of predicting poor outcomes in validation set, the area under the curve (AUC) was 0.727 for the DWI radiomics model, 0.821 for the ADC radiomics model, 0.825 for the DWI + ADC radiomics model, and 0.808 for the multi-clinical model. Furthermore, a prediction model was built using all selected features, the AUC for predicting poor outcomes increased to 0.86.

Conclusion

Radiomics features extracted from DWI and ADC images can serve as valuable biomarkers for predicting poor clinical outcomes in patients with AIS. Furthermore, when these radiomics features were combined with multi-clinical features, the predictive performance was enhanced. The prediction model has the potential to provide guidance for tailoring rehabilitation therapies based on individual patient risks for poor outcomes.
High vs. low tidal volume and pulmonary complications in patients with cervical spinal cord injury on mechanical ventilation: systematic review
Authors: Meregildo-Rodríguez Edinson Dante , Vásquez-Tirado Gustavo Adolfo , Quispe-Castañeda Claudia Vanessa , del Carmen Cuadra-Campos María , Contreras-Cabrera Jhuliana M. , Pinedo-Portilla Juan Luis
Published: March 2024

Introduction

Cervical spinal cord injury (CSCI) patients on mechanical ventilation often lack standardized guidelines for optimal ventilatory support. This study reviews existing literature to compare outcomes between high tidal volume (HTV) and low tidal volume (LTV) strategies in this unique patient population.

Methods

We searched for studies published up to August 30, 2023, in five databases, following a PECO/PICO strategy. We found six studies for quantitative analysis and meta-analyzed five studies.

Results

This meta-analysis included 396 patients with CSCI and mechanical ventilation (MV), 119 patients treated with high tidal volume (HTV), and 277 with low tidal volume (LTV). This first meta-analysis incorporates the few studies that show contradictory findings. Our meta-analysis shows that there is no significant statistical difference in developing VAP between both comparison groups (HTV vs. LTV) (OR 0.46; 95% CI 0.13 to 1.66; p > 0.05; I2: 0%), nor are there differences between the presence of other pulmonary complications when treating with HTV such as acute respiratory distress syndrome (ARDS), atelectasis, onset of weaning.

Conclusion

In patients with CSCI in MV, the use of HTV does not carry a greater risk of pneumonia compared to LTV; in turn, it is shown as a safe ventilatory strategy as it does not establish an increase in other pulmonary complications such as ARDS, atelectasis, the onset of weaning nor others associated with volutrauma. It is necessary to evaluate the role of HTV ventilation in this group of patients in primary RCT-type studies.
Exploring injury assessment using motion tracking technology and ergonomic tools in archery performance
Authors: Ji Xiaoxu , Arzalluz Irati , Gao Xin , Al Tamimi Zainab , Swierski Ethan , Piovesan Davide
Published: March 2024

Introduction: Archers face a notable annual risk of injury attributed to the repetitive strain exerted on their upper body muscles and joints. This groundbreaking study marks the first utilization of real-time motion tracking combined with ergonomic analysis in archery research, eliminating the need for extensive equipment setups and streamlining data collection.

Methods: Thirteen participants were involved, undertaking four tasks with varying draw weights of bows and target positions.

Results: The precise recording of human movements revealed distinctive postures adopted by both genders across different tasks. Furthermore, an assessment of exposed spinal force and its correlation with anatomical variables was conducted, providing valuable insights into injury risks during archery performances.
Unveiling the affinity–stability relationship in anti-measles virus antibodies: a computational approach for hotspots prediction
Authors: Paul Rimpa , Kasahara Keisuke , Sasaki Jiei , Pérez Jorge Fernández , Matsunaga Ryo , Hashiguchi Takao , Kuroda Daisuke , Tsumoto Kouhei
Published: March 2024

Recent years have seen an uptick in the use of computational applications in antibody engineering. These tools have enhanced our ability to predict interactions with antigens and immunogenicity, facilitate humanization, and serve other critical functions. However, several studies highlight the concern of potential trade-offs between antibody affinity and stability in antibody engineering. In this study, we analyzed anti-measles virus antibodies as a case study, to examine the relationship between binding affinity and stability, upon identifying the binding hotspots. We leverage in silico tools like Rosetta and FoldX, along with molecular dynamics (MD) simulations, offering a cost-effective alternative to traditional in vitro mutagenesis. We introduced a pattern in identifying key residues in pairs, shedding light on hotspots identification. Experimental physicochemical analysis validated the predicted key residues by confirming significant decrease in binding affinity for the high-affinity antibodies to measles virus hemagglutinin. Through the nature of the identified pairs, which represented the relative hydropathy of amino acid side chain, a connection was proposed between affinity and stability. The findings of the study enhance our understanding of the interactions between antibody and measles virus hemagglutinin. Moreover, the implications of the observed correlation between binding affinity and stability extend beyond the field of anti-measles virus antibodies, thereby opening doors for advancements in antibody research.
Video head impulse test in stroke: a review of published studies
Authors: Jaganathan Niranjana , Mohamed Mohd Hazmi , Md Pauzi Ahmad Luqman , Mahayidin Hasni , Hanapai Ahmad Firdaus , Wan Sulaiman Wan Aliaa , Basri Hamidon , Inche Mat Liyana
Published: March 2024

Accurate and timely diagnosis of posterior circulation stroke in patients with acute dizziness is a challenge that can lead to misdiagnosis and significant harm. The present review sought to identify and describe published research on the clinical application of vHIT in posterior circulation stroke. vHIT, a portable device, has gained prominence in evaluating peripheral vestibular disorders and offers potential applications in diagnosing neurological disorders, particularly posterior circulation stroke. Several studies have shown that vHIT can differentiate between stroke and vestibular neuritis based on VOR gain values, with high sensitivity and specificity. The manuscript also discusses vHIT’s performance in differentiating between types of posterior circulation stroke, such as PICA, AICA, and SCA strokes. While vHIT has demonstrated promise, the review emphasizes the need for further research to validate its use as a tool to rule out stroke in acute dizziness patients in the emergency department. In conclusion, the manuscript underscores the potential of vHIT as a valuable addition to the diagnostic arsenal for acute dizziness, particularly in the context of posterior circulation stroke. It calls for further research and wider adoption of vHIT in clinical settings to improve patient care and reduce unnecessary costs associated with misdiagnoses.
Differences in the amplitude of low-frequency fluctuations of spontaneous brain activity between preterm and term infants
Authors: Feng Ye , Wang Yuanchong , Li Xu , Dai Liying , Zhang Jian
Published: March 2024

Objectives

To date, the majority of research on resting-state functional magnetic resonance imaging (rs-fMRI) in the developing brain has primarily centered on adolescents and adults, leaving a gap in understanding variations in spontaneous brain activity at rest in preterm infants. This study aimed to uncover and comprehend the distinctions in spontaneous brain activity between preterm and term infants, with the goal of establishing a foundation for assessing the condition of preterm infants.

Methods

In this study, 14 term infants and 15 preterm infants with equivalent gestational age were carefully chosen from the neonatal unit of Anhui Provincial Children’s Hospital. The amplitude of low-frequency fluctuations (ALFF) intensity was assessed using resting-state functional magnetic resonance imaging (rs-fMRI) to examine brain activity in both groups. Subsequently, the differences between the term and preterm infants were statistically analyzed using a two-sample t-test. A p-value of <0.05, corrected for the REST Gaussian Random Fields, was deemed to be statistically significant.

Results

In comparison to the term infant group, the preterm infant group exhibited a significant increase in the ALFF value in the left precuneus, left frontal superior orbital gyrus, and left calcarine cortex.

Conclusion

Significant variances in spontaneous brain activity have been observed in various regions between term infants and preterm infants of equivalent gestational age. These variations could potentially impact the emotional and cognitive development of preterm infants in the long term.
Structural changes in the retina as a potential biomarker in Parkinson's disease: an approach from optical coherence tomography
Authors: Poveda Santiago , Arellano Ximena , Bernal-Pacheco Oscar , Valencia López Alejandro
Published: March 2024

Introduction

Parkinson's disease (PD) presents challenges in early diagnosis and follow-up due to the lack of characteristic findings. Recent studies suggest retinal changes in PD are possibly indicative of neurodegeneration. We explored these changes using optical coherence tomography (OCT) to assess retinal nerve fiber layer (RNFL) and ganglion cell complex (GCC) thickness.

Methods

Thirty PD and non-PD patients were matched according to demographic characteristics and OCT and clinical evaluations to rule out other neurodegenerative and visual diseases.

Results

We observed a significant thinning of the RNFL in patients diagnosed with PD compared to non-PD patients (p = 0.015). Additionally, this reduction in RNFL thickness was found to correlate with the severity of the disease (p = 0.04).

Conclusion

The OCT serves as a tool for quantifying neurodegeneration in PD, showing a significant correlation with disease severity. These findings suggest that OCT could play a crucial role as a potential biomarker in the diagnosis and monitoring of PD.
Acupuncture modulates the gut microbiota in Alzheimer’s disease: current evidence, challenges, and future opportunities
Authors: Yan Long , Li Hong , Qian Yulin , Liu Qidi , Cong Shan , Dou Baomin , Wang Yu , Wang Meng , Yu Tao
Published: March 2024

Alzheimer’s disease, one of the most severe and common neurodegenerative diseases, has no effective cure. Therefore it is crucial to explore novel and effective therapeutic targets. The gut microbiota - brain axis has been found to play a role in Alzheimer’s disease by regulating the neuro-immune and endocrine systems. At the same time, acupuncture can modulate the gut microbiota and may impact the course of Alzheimer’s disease. In this Review, we discuss recent studies on the role of acupuncture on the gut microbiota as well current challenges and future opportunities of acupuncture as potential treatment for the prevention and treatment of Alzheimer’s disease.
Effect of different modalities of transcranial magnetic stimulation on Parkinson’s patients cognitive impairment and long-term effectiveness: a systematic review and network meta-analysis
Authors: Yang Yulin , Yan Zhenyang , Chang Wanpeng , Ding Jiangtao , Xu Hongli
Published: March 2024

Objective

This study used network Meta-analysis to compare the effects of different transcranial magnetic stimulation (TMS) modalities on the effectiveness and long-term validity of improving cognitive function in Parkinson’s patients.

Methods

Computer searches of the Cochrane Library, PubMed, Web of Science, Embass, CNKI and Wanfang Data were conducted to collect randomized controlled clinical studies on TMS to improve cognitive function in Parkinson’s patients published from the time of library construction to December 2023.

Results

A total of 22 studies and 1,473 patients were included, comprising 5 interventions: high frequency repetitive transcranial magnetic stimulation (HF-rTMS), low frequency repetitive transcranial magnetic stimulation (LF-rTMS), intermittent theta burst stimulation (iTBS), sham stimulation and conventional rehabilitation therapy (CRT). Network Meta-analysis showed that the ranking results of different TMS intervention modalities in terms of MoCA scores were: HF-rTMS > LF-rTMS > iTBS > sham > CRT, the ranking results of different TMS intervention modalities in terms of MMSE scores were: HF-rTMS > LF-rTMS > sham > CRT. The effect of TMS on improving Parkinsonian cognitive function lasted for 1 month compared to the no-stimulation group.

Conclusion

TMS has some long-term sustained effects on improving cognitive function in Parkinson’s patients. HF-rTMS is more effective in improving cognitive function in Parkinson’s patients.

Systematic review registration: https://www.crd.york.ac.uk/PROSPERO, identifier: CRD42023463958.
Agreement between parent-report and EMR height, weight, and BMI among rural children
Authors: Potts Benjamin A. , Wood G. Craig , Bailey-Davis Lisa
Published: March 2024

Introduction

Remote anthropometric surveillance has emerged as a strategy to accommodate lapses in growth monitoring for pediatricians during coronavirus disease 2019 (COVID-19). The purpose of this investigation was to validate parent-reported anthropometry and inform acceptable remote measurement practices among rural, preschool-aged children.

Methods

Parent-reported height, weight, body mass index (BMI), BMI z-score, and BMI percentile for their child were collected through surveys with the assessment of their source of home measure. Objective measures were collected by clinic staff at the child’s well-child visit (WCV). Agreement was assessed using correlations, alongside an exploration of the time gap (TG) between parent-report and WCV to moderate agreement. Using parent- and objectively reported BMI z-scores, weight classification agreement was evaluated. Correction equations were applied to parent-reported anthropometrics.

Results

A total of 55 subjects were included in this study. Significant differences were observed between parent- and objectively reported weight in the overall group (−0.24 kg; p = 0.05), as well as height (−1.8 cm; p = 0.01) and BMI (0.4 kg/m2; p = 0.02) in the ≤7d TG + Direct group. Parental reporting of child anthropometry ≤7d from their WCV with direct measurements yielded the strongest correlations [r = 0.99 (weight), r = 0.95 (height), r = 0.82 (BMI), r = 0.71 (BMIz), and r = 0.68 (BMI percentile)] and greatest classification agreement among all metrics [91.67% (weight), 54.17% (height), 83.33% (BMI), 91.67% (BMIz), and 33.33% (BMI percentile)]. Corrections did not remarkably improve correlations.

Discussion

Remote pediatric anthropometry is a valid supplement for clinical assessment, conditional on direct measurement within 7 days. In rural populations where socioenvironmental barriers exist to care and surveillance, we highlight the utility of telemedicine for providers and researchers.
Preoperative plasma fibrinogen and C-reactive protein/albumin ratio as prognostic biomarkers for pancreatic carcinoma
Authors: Chen Xiaopeng , Chen Zhaohui , Guo Jianyang , Xiu Zhe , Chen Huangxiang
Published: March 2024

Objective

Pancreatic carcinoma is characterised by high aggressiveness and a bleak prognosis; optimising related treatment decisions depends on the availability of reliable prognostic markers. This study was designed to compare various blood biomarkers, such as neutrophil/lymphocyte ratio (NLR), lymphocyte/monocyte ratio (LMR), platelet/lymphocyte ratio (PLR), C-reactive protein (CRP), albumin (Alb), plasma fibrinogen (PF), and CRP/Alb in patients with pancreatic carcinoma.

Methods

Our study retrospectively reviewed 250 patients with pancreatic carcinoma diagnosed between July 2007 and December 2018. The Cutoff Finder application was used to calculate the optimal values of CRP/Alb and PF. The Chi-square test or Fisher’s exact test was used to analyse the correlation of CRP/Alb and PF with other clinicopathological factors. Conducting univariate and multivariate analyses allowed further survival analysis of these prognostic factors.

Results

Multivariate analysis revealed that, in a cohort of 232 patients with pancreatic ductal adenocarcinoma (PDAC), the PF level exhibited statistical significance for overall survival (hazard ratio (HR) = 0.464; p = 0.023); however, this correlation was not found in the entire group of 250 patients with pancreatic carcinoma. Contrastingly, the CRP/Alb ratio was demonstrated statistical significance in both the entire pancreatic carcinoma cohort (HR = 0.471; p = 0.026) and the PDAC subgroup (HR = 0.484; p = 0.034). CRP/Alb and PF demonstrated a positive association (r=0.489, p<0.001) as indicated by Spearman’s rank correlation analysis. Additionally, in 232 PDAC patients, the combination of the CRP/Alb ratio and PF had synergistic effects on prognosis when compared with either the CRP/Alb ratio or the PF concentration alone.

Conclusion

PF concentration is a convenient, rapid, and noninvasive biomarker, and its combination with the CRP/Alb ratio could significantly enhance the accuracy of prognosis prediction in pancreatic carcinoma patients, especially those with the most common histological subtype of PDAC.
DNA damage levels in peripheral blood mononuclear cells before and after first cycle of chemotherapy have comparable prognostic values in germ cell tumor patients
Authors: Ivovič Danica , Šestáková Zuzana , Roška Jan , Kálavská Katarína , Hurbanová Lenka , Holíčková Andrea , Smolková Božena , Kabelíková Pavlína , Novotná Věra , Chovanec Michal , Palacka Patrik , Mego Michal , Jurkovičová Dana , Chovanec Miroslav
Published: March 2024

Background

Germ cell tumors (GCTs) represent the most frequent solid malignancy in young men. This malignancy is highly curable by cisplatin (CDDP)-based chemotherapy. However, there is a proportion of patients having a poor prognosis due to refractory disease or its relapse. No reliable biomarkers being able to timely and accurately stratify poor prognosis GCT patients are currently available. Previously, we have shown that chemotherapy-naïve GCT patients with higher DNA damage levels in peripheral blood mononuclear cells (PBMCs) have significantly worse prognosis compared to patients with lower DNA damage levels.

Methods

DNA damage levels in PBMCs of both chemotherapy-naïve and first cycle chemotherapy-treated GCT patients have been assessed by standard alkaline comet assay and its styrene oxide (SO)-modified version. These levels were correlated with clinico-pathological characteristics.

Results

We re-confirm prognostic value of DNA damage level in chemotherapy-naïve GCT patients and reveal that this prognosticator is equally effective in GCT patients after first cycle of CDDP-based chemotherapy. Furthermore, we demonstrate that SO-modified comet assay is comparably sensitive as standard alkaline comet assay in case of patients who underwent first cycle of CDDP-based chemotherapy, although it appears more suitable to detect DNA cross-links.

Conclusion

We propose that DNA damage levels in PBMCs before and after first cycle of CCDP-based chemotherapy are comparable independent prognosticators for progression-free and overall survivals in GCT patients. Therefore, their clinical use is highly advised to stratify GCT patients to identify those who are most at risk of developing disease recurrence or relapse, allowing tailoring therapeutic interventions to poor prognosis individuals, and optimizing their care management and treatment regimen.