bluuebunny/crossref_metadata_2025 huggingface dataset:
Emergence of Nontuberculous Mycobacteria at the Human–Livestock–Environment Interface in Zambia
Authors: Zulu Mildred , Malama Sydney , Monde Ngula , Kainga Henson , Tembo Rabecca , Mwaba Florence , Saad Shereen Ahmed , Daka Victor , Mukubesa Andrew N. , Ndebe Joseph , Shambaba Obi , Munyeme Musso
Published: March 2023

The prevalence of nontuberculous mycobacteria (NTM) infections and disease is rising worldwide due to increased research, diagnostics capabilities, and awareness of the disease. There is limited prevalence data for NTM from different sources in Zambia. The aim of this study was to determine the prevalence and species distribution of NTM at the human–livestock–environment interface. A cross-section study was conducted in Namwala, Chipata, and Lundazi Districts of Zambia from April 2020 to December 2021. Sputum samples were collected from tuberculosis presumptive patients from different health centers, cattle tissues were collected from different abattoirs during routine post-mortem, and water samples were collected from different drinking points for humans and animals such as taps, boreholes, wells, rivers, dams and ponds, and then cultured following standard mycobacteriology procedures. Capilia TB-Neo assay was used to identify NTM from the positive cultures. DNA was extracted and the 16S to 23S rRNA (internal transcribed spacer region) (ITS) was amplified and sequenced to identify the species. The overall prevalence of NTM from humans, cattle, and water was 9.1% (72/794, 95% CI 7.2–11.3). The prevalence in humans was 7.8% (33/421, 95% CI 5.54–10.94), in cattle it was 10.6% (15/142, 95% CI 6.2–17.1), and in water it was 10.4% (24/231, 95% CI 6.9–15.2). Our study has shown, for the first time in Zambia, simultaneous isolation of NTM at the human–livestock–environment interface; M. avium complex and M. fortuitum were the most commonly isolated species. M. fortuitum and M. gordonae were isolated from all three sources, while M. abscessus was isolated from humans and water. The isolation of similar NTM species at the interface which are potentially pathogenic is a public health problem which merits further investigation.
Molecular Typing of Mastadenoviruses in Simultaneously Collected Nasopharyngeal Swabs and Stool Samples from Children Hospitalized for Acute Bronchiolitis, Acute Gastroenteritis, and Febrile Seizures
Authors: Biškup Urška Glinšek , Steyer Andrej , Lusa Lara , Strle Franc , Pokorn Marko , Mrvič Tatjana , Grosek Štefan , Petrovec Miroslav , Jevšnik Virant Monika
Published: March 2023

This study determines and compares the frequency of human mastadenovirus (HAdV) presence in children with acute bronchiolitis (AB), acute gastroenteritis (AGE), and febrile seizures (FS), ascertains types of HAdVs associated with each individual syndrome and contrasts the findings with a control group of children. The presence of HAdVs was ascertained in simultaneously collected nasopharyngeal (NP) swabs and stool samples amplifying the hexon gene by RT-PCR; these were sequenced to determine the types of HAdVs. HAdVs were grouped into eight different genotypes. Of these, three (F40, F41, and A31) were found solely in stool samples, whereas the others (B3, C1, C2, C5, and C6) were found in both stool samples and NP swabs. The most common genotypes in NP swabs were C2 (found in children with AGE and FS) and C1 (only in children with FS), whereas in stool samples genotypes F41 (in children with AGE) and C2 (in children with AGE and FS) prevailed, and C2 was simultaneously present in both samples. HAdVs were more often detected in stool samples than in NP swabs in patients (with the highest estimated viral load in stool samples in children with AB and AGE) and healthy controls and were more common in NP swabs in children with AGE than in children with AB. In most patients, the characterized genotypes in NP swabs and stool samples were in concordance.
Isolation and Characterisation of Electrogenic Bacteria from Mud Samples
Authors: Schneider György , Pásztor Dorina , Szabó Péter , Kőrösi László , Kishan Nandyala Siva , Raju Penmetsa Appala Rama Krishna , Calay Rajnish Kaur
Published: March 2023

To develop efficient microbial fuel cell systems for green energy production using different waste products, establishing characterised bacterial consortia is necessary. In this study, bacteria with electrogenic potentials were isolated from mud samples and examined to determine biofilm-formation capacities and macromolecule degradation. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry identifications have revealed that isolates represented 18 known and 4 unknown genuses. They all had the capacities to reduce the Reactive Black 5 stain in the agar medium, and 48 of them were positive in the wolfram nanorod reduction assay. The isolates formed biofilm to different extents on the surfaces of both adhesive and non-adhesive 96-well polystyrene plates and glass. Scanning electron microscopy images revealed the different adhesion potentials of isolates to the surface of carbon tissue fibres. Eight of them (15%) were able to form massive amounts of biofilm in three days at 23 °C. A total of 70% of the isolates produced proteases, while lipase and amylase production was lower, at 38% and 27% respectively. All of the macromolecule-degrading enzymes were produced by 11 isolates, and two isolates of them had the capacity to form a strong biofilm on the carbon tissue one of the most used anodic materials in MFC systems. This study discusses the potential of the isolates for future MFC development applications.
Iron Status and Supplementation during Tuberculosis
Authors: Nienaber Arista , Uyoga Mary A. , Dolman-Macleod Robin C. , Malan Linda
Published: March 2023

Tuberculosis (TB) is characterised by chronic non-resolving inflammation. The effects of the host immune and inflammatory response to reduce iron acquisition by the bacteria, together with other contributing factors, predispose TB patients to anaemia of infection and iron deficiency anaemia (IDA). The presence of anaemia in TB patients has been linked to poor clinical outcomes. However, due to the reliance of the bacteria on iron, the management of anaemia in TB is complicated, and anaemia of infection is likely to resolve with correct TB drug treatment. On the other hand, IDA may require iron supplementation. This review aims to describe iron metabolism in TB and how this contributes to the development of iron deficiency and anaemia. Additionally, we summarise the evidence on the association between iron status and clinical outcomes as well as the available preclinical and clinical trials on iron supplementation in TB.
A Molecular Dynamics Simulation for Thermal Activation Process in Covalent Bond Dissociation of a Crosslinked Thermosetting Polymer
Authors: Yamada Naoki , Oya Yutaka , Kato Nobuhiko , Mori Kazuki , Koyanagi Jun
Published: March 2023

A novel algorithm for covalent bond dissociation is developed to accurately predict fracture behavior of thermosetting polymers via molecular dynamics simulation. This algorithm is based on the Monte Carlo method that considers the difference in local strain and bond-dissociation energies to reproduce a thermally activated process in a covalent bond dissociation. This study demonstrates the effectiveness of this algorithm in predicting the stress–strain relationship of fully crosslinked thermosetting polymers under uniaxial tensile conditions. Our results indicate that the bond-dissociation energy plays an important role in reproducing the brittle fracture behavior of a thermosetting polymer by affecting the number of covalent bonds that are dissociated simultaneously.
Systematic Study of Resveratrol Nanoliposomes Transdermal Delivery System for Enhancing Anti-Aging and Skin-Brightening Efficacy
Authors: Zhang Xinchao , Chen Siyuan , Luo Dan , Chen Dan , Zhou Hong , Zhang Shuting , Chen Xuan , Lu Wangwang , Liu Wei
Published: March 2023

Due to the stratum corneum barrier, resveratrol is difficult to be absorbed transdermally, limiting its anti-aging and skin-brightening effects. Furthermore, there is a lack of systematic studies on the efficacy of resveratrol in human skin, especially in three-dimensional skin models and clinical trials. To overcome the low transdermal delivery issue, we encapsulated resveratrol into nanoliposomes using the high-pressure homogenization method to develop an efficient transdermal drug delivery system, and systematically evaluated its anti-aging and skin-brightening efficacy via cell line models, a three-dimensional skin model and human skin. The resveratrol nanoliposomes effectively improved the transdermal penetration and retention of resveratrol and enhanced cellular uptake. In addition, compared to free resveratrol, resveratrol nanoliposomes remarkably enhanced the skin-care effects by promoting the antioxidant capacity and collagen synthesis, inhibiting the secretion of matrix metalloproteinases, tyrosine activity and melanin synthesis. Notably, human clinical trials proved the anti-wrinkle and skin-brightening effectiveness of resveratrol nanoliposomes. Three levels of systematic studies indicated that resveratrol nanoliposomes could be a promising transdermal drug delivery system to enhance the anti-aging and skin-brightening effects of resveratrol.
Applications of Polyacetylene Derivatives in Gas and Liquid Separation
Authors: Chen Manyu , Hu Guangze , Shen Tanxiao , Zhang Haoke , Sun Jing Zhi , Tang Ben Zhong
Published: March 2023

As a low energy consumption, simple operation and environmentally friendly separation method, membrane separation has attracted extensive attention. Therefore, researchers have designed and synthesized various types of separation membrane, such as metal organic framework (MOF), covalent organic framework (COF), polymer of intrinsic micro-porosity (PIM) and mixed matrix membranes. Some substituted polyacetylenes have distorted structures and formed micropores due to the existence of rigid main chains and substituted side groups, which can be applied to the field of membrane separation. This article mainly introduces the development and application of substituted polyacetylenes in gas separation and liquid separation based on membrane technology.
Enhanced Ammonia Decomposition by Tuning the Support Properties of Ni/GdxCe1-xO2-δ at 600 °C
Authors: He Haihua , Chen Chonglai , Bian Chaoqun , Ren Junhua , Liu Jiajia , Huang Wei
Published: March 2023

Ammonia decomposition is a promising method to produce high-purity hydrogen. However, this process typically requires precious metals (such as Ru, Pt, etc.) as catalysts to ensure high efficiency at relatively low temperatures. In this study, we propose using several Ni/GdxCe1-xO2-δ catalysts to improve ammonia decomposition performance by adjusting the support properties. We also investigate the underlying mechanism for this enhanced performance. Our results show that Ni/Ce0.8Gd0.2O2-δ at 600 °C can achieve nearly complete ammonia decomposition, resulting in a hydrogen production rate of 2008.9 mmol.g−1.h−1 with minimal decrease over 150 h. Density functional theory calculations reveal that the recombinative desorption of nitrogen is the rate-limiting step of ammonia decomposition over Ni. Our characterizations indicate that Ni/Ce0.8Gd0.2O2-δ exhibits a high concentration of oxygen vacancies, highly dispersed Ni on the surface, and abundant strong basic sites. These properties significantly enhance the associative desorption of N and strengthen the metal support interactions, resulting in high catalytic activity and stability. We anticipate that the mechanism could be applied to designing additional catalysts with high ammonia decomposition performance at relatively low temperatures.
Xanthan-Based Materials as a Platform for Heparin Delivery
Authors: Anghel Narcis , Apostol Irina , Dinu Maria Valentina , Dimitriu Cristina Daniela , Spiridon Iuliana , Verestiuc Liliana
Published: March 2023

Heparin (Hep), with its anticoagulant activity, antiangiogenic and apoptotic effects, and growth factor binding, plays an important role in various biological processes. Formulations as drug delivery systems protect its biological activity, and limit the potential side effects of faulty administration. The objective of this study was to develop novel xanthan-based materials as a delivery carrier for heparin. The materials exhibited remarkable elastic behavior and toughness without any crack development within the network, which also support their application for tissue engineering. It was found that all materials possessed the ability to control the release of heparin, according to the Korsmeyer–Peppas release model. All Hep-containing materials caused significant exchanges of the activated partial thromboplastin time (aPTT) and prothrombin time (PT) parameters, indicating that formulated natural/natural synthetic polymeric networks conserved heparin’s biological activity and its ability to interrupt the blood coagulation cascade. The obtained results confirmed that developed materials could be carriers for the controlled release of heparin, with potential applications in topical administration.
Green Synthesis, Characterization, Antioxidant, Antibacterial and Enzyme Inhibition Effects of Chestnut (Castanea sativa) Honey-Mediated Silver Nanoparticles
Authors: Keskin Merve , Kaya Gülşen , Bayram Sinan , Kurek-Górecka Anna , Olczyk Paweł
Published: March 2023

In this study, chestnut honey-based silver nanoparticles (CH-AgNPs) were synthesized at different temperatures (30, 60 and 90 °C) and these nanoparticles were characterized by different techniques such as UV–vis spectrophotometer, Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and energy dispersive X-ray (EDX). The DPPH free radical scavenging assay was used to determine the antioxidant activity of the obtained nanoparticles. The inhibition effects of these nanoparticles for some clinically important enzymes such as myeloperoxidase and collagenase were investigated. In addition, the disk diffusion method (DDM), agar well diffusion (AWD), and minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) techniques were used to determine the antibacterial activity of CH-AgNPs. In honey-based silver nanoparticle production processes using green synthesis, it was determined that the nanoparticle sizes decreased from 55 to 27 nm with an increase in temperature. In addition, it was determined that the rate of inhibition of myeloperoxidase (36.4% to 34.0%) and collagenase enzymes (74.2% to 68.7%) increased with a decrease in particle size. As a result of the antibacterial activity tests, it was observed that CH-AgNPs have antibacterial activity against all target pathogens including Gram-positive and Gram-negative bacteria. The obtained results show that CH-AgNPs produced using chestnut honey have the potential to be used in fields such as medicine, pharmacy and cosmetic technology.
Different Extraction Approaches for the Analysis of Melatonin from Cabernet Sauvignon and Feteasca Neagra Wines Using a Validated HPLC-FL Method
Authors: Eremia Sandra A. V. , Albu Camelia , Radu Gabriel L. , Ion Marian
Published: March 2023

In recent years, the wine industry has shown a considerable degree of interest in the occurrence of melatonin in wines. Sample pretreatment may be the most important step in trace analysis. Since wine is a complex matrix and melatonin is present in low amounts (ppb), an adequate extraction technique is required. In this study, the effect of several extraction methods, such as solid phase extraction (SPE), Quick, Easy, Cheap, Effective, Rugged, and Safe extraction (QuEChERS), and dispersive liquid–liquid micro-extraction (DLLME) was studied and the variable parameters that can arise throughout the extraction process were optimized to obtain the best results. A high-performance liquid chromatography with fluorescence detector (HPLC-FL) method was adapted and validated, including measurement uncertainty, for the analysis of melatonin in wines and to assess the efficiency of the extraction yield. After comparing the acquired results, the DLLME method was optimized. Extraction recoveries values ranging from 95 to 104% demonstrated that the approach may be successfully applied for the extraction and concentration (enrichment factor of almost eight) of melatonin in wine samples prior to HPLC-FL analysis. The first report of melatonin levels in Feteasca Neagra wines has been made. The data obtained for Cabernet Sauvignon revealed that the final levels of melatonin in the wines are dependent on the winemaking process.
Analysis of Milk Microstructure Using Raman Hyperspectral Imaging
Authors: Surkova Anastasiia , Bogomolov Andrey
Published: March 2023

Optical spectroscopic analysis of the chemical composition of milk in its natural state is complicated by a complex colloidal structure, represented by differently sized fat and protein particles. The classical techniques of molecular spectroscopy in the visible, near-, and mid-infrared ranges carry only bulk chemical information about a sample, which usually undergoes a destructive preparation stage. The combination of Raman spectroscopy with confocal microscopy provides a unique opportunity to obtain a vibrational spectrum at any single point of the sample volume. In this study, scanning confocal Raman microscopy was applied for the first time to investigate the chemical microstructure of milk using samples of various compositions. The obtained hyperspectral images of selected planes in milk samples are represented by three-dimensional data arrays. Chemometric data analysis, in particular the method of multivariate curve resolution, has been used to extract the chemical information from complex partially overlaid spectral responses. The results obtained show the spatial distribution of the main chemical components, i.e., fat, protein, and lactose, in the milk samples under study using intuitive graphical maps. The proposed experimental and data analysis method can be used in an advanced chemical analysis of natural milk and products on its basis.
Rape Straw Supported FeS Nanoparticles with Encapsulated Structure as Peroxymonosulfate and Hydrogen Peroxide Activators for Enhanced Oxytetracycline Degradation
Authors: Wang Guiyin , Yang Yan , Xu Xiaoxun , Zhang Shirong , Yang Zhanbiao , Cheng Zhang , Xian Junren , Li Ting , Pu Yulin , Zhou Wei , Xiang Gang , Pu Zhien
Published: March 2023

Iron-based catalysts with high load content of iron sulfide (FeS) were commonly peroxymonosulfate (PMS) and hydrogen peroxide (H2O2) activators to degrade organic pollutants but limited catalytic efficiency and increased risk of ferrous ion leaching restricted their use. Meanwhile, various biomass materials such as straw, peel, and branch have been extensively prepared into biochar for mechanical support for iron-based catalysts; however, the preparation process of biochar was energy-intensive. In this study, FeS nanoparticles modified rape straw composites (RS–FeS) encapsulated with ethylenediaminetetraacetic acid (RS–EDTA–FeS) were successfully presented by in-situ synthesis method for efficiently activating PMS and H2O2 to degrade oxytetracycline (OTC), which was economical and environmentally friendly. The results showed that the modified rape straw can remove OTC efficiently, and the addition of EDTA also significantly enhanced the stability and the reusability of the catalyst. In addition, EDTA also promoted the activation of H2O2 at neutral pH. The OTC degradation efficiency of the two catalysts by PMS was faster than that of H2O2, but H2O2 had a stronger ability to remove OTC than PMS. The highest OTC removal efficiency of RS–FeS and RS–EDTA–FeS were 87.51 and 81.15%. O2•– and 1O2 were the major reactive oxidative species (ROS) in the PMS system. Furthermore, compared with RS–FeS, the addition of EDTA inhabited the generation of O2•– in the PMS system. Instead, O2•– and •OH were the major ROS in the H2O2 system, but 1O2 was also identified in RS–FeS/H2O2 system. RS–EDTA–FeS showed a trend of rising first and then decreasing in recycle test. Instead, the removal rate of OTC by RS–FeS decreased significantly with the increase in reuse times. In the actual wastewater test, the TOC removal of two catalysts active by H2O2 was better than PMS, which was consistent with the test results of OTC, indicating that the two catalysts have application value in the removal of organic pollutants in actual wastewater. This study directly used plant materials as catalysts and omits the preparation process of biochar, greatly reduces the preparation cost and secondary pollution of catalysts, and provides theoretical support for the deepening of advanced oxidation technology.
An Overview on Carbon Quantum Dots Optical and Chemical Features
Authors: Giordano Marco Giuseppe , Seganti Giulia , Bartoli Mattia , Tagliaferro Alberto
Published: March 2023

Carbon quantum dots are the materials of a new era with astonishing properties such as high photoluminescence, chemical tuneability and high biocompatibility. Since their discovery, carbon quantum dots have been described as nanometric high-fluorescent carbon nanoparticles, but this definition has become weaker year after year. Nowadays, the classification and the physical explanation of carbon quantum dots optical properties and their chemical structure remain matter of debate. In this review, we provide a clear discussion on these points, providing a starting point for the rationalization of their classification and a comprehensive view on the optical and chemical features of carbon quantum dots.
Monodisperse MoS2/Graphite Composite Anode Materials for Advanced Lithium Ion Batteries
Authors: Liu Baosheng , Li Feng , Li Hongda , Zhang Shaohui , Liu Jinghua , He Xiong , Sun Zijun , Yu Zhiqiang , Zhang Yujin , Huang Xiaoqi , Guo Fei , Wang Guofu , Jia Xiaobo
Published: March 2023

Traditional graphite anode material typically shows a low theoretical capacity and easy lithium decomposition. Molybdenum disulfide is one of the promising anode materials for advanced lithium-ion batteries, which possess low cost, unique two-dimensional layered structure, and high theoretical capacity. However, the low reversible capacity and the cycling-capacity retention rate induced by its poor conductivity and volume expansion during cycling blocks further application. In this paper, a collaborative control strategy of monodisperse MoS2/graphite composites was utilized and studied in detail. MoS2/graphite nanocomposites with different ratios (MoS2:graphite = 20%:80%, 40%:60%, 60%:40%, and 80%:20%) were prepared by mechanical ball-milling and low-temperature annealing. The graphite sheets were uniformly dispersed between the MoS2 sheets by the ball-milling process, which effectively reduced the agglomeration of MoS2 and simultaneously improved the electrical conductivity of the composite. It was found that the capacity of MoS2/graphite composites kept increasing along with the increasing percentage of MoS2 and possessed the highest initial discharge capacity (832.70 mAh/g) when MoS2:graphite = 80%:20%. This facile strategy is easy to implement, is low-cost, and is cosmically produced, which is suitable for the development and manufacture of advance lithium-ion batteries.
Effect of Chemical Bath Deposition Variables on the Properties of Zinc Sulfide Thin Films: A Review
Authors: Arsad Akmal Zaini , Zuhdi Ahmad Wafi Mahmood , Abdullah Siti Fazlili , Chau Chien Fat , Ghazali Azrul , Ahmad Ibrahim , Abdullah Wan Syakirah Wan
Published: March 2023

Zinc sulfide (ZnS) thin films prepared using the chemical bath deposition (CBD) method have demonstrated great viability in various uses, encompassing photonics, field emission devices, field emitters, sensors, electroluminescence devices, optoelectronic devices, and are crucial as buffer layers of solar cells. These semiconducting thin films for industrial and research applications are popular among researchers. CBD appears attractive due to its simplicity, cost-effectiveness, low energy consumption, low-temperature compatibility, and superior uniformity for large-area deposition. However, numerous parameters influence the CBD mechanism and the quality of the thin films. This study offers a comprehensive review of the impact of various parameters that can affect different properties of ZnS films grown on CBD. This paper provides an extensive review of the film growth and structural and optical properties of ZnS thin films influenced by various parameters, which include complexing agents, the concentration ratio of the reactants, stirring speed, humidity, deposition temperature, deposition time, pH value, precursor types, and annealing temperature environments. Various studies screened the key influences on the CBD parameters concerning the quality of the resulting films. This work will motivate researchers to provide additional insight into the preparation of ZnS thin films using CBD to optimize this deposition method to its fullest potential.
One-Pot Synthesis of Isoxazole-Fused Tricyclic Quinazoline Alkaloid Derivatives via Intramolecular Cycloaddition of Propargyl-Substituted Methyl Azaarenes under Metal-Free Conditions
Authors: Wang Zhuo , Zhao Yuhan , Chen Jiaxin , Chen Mengyao , Li Xuehan , Jiang Ting , Liu Fang , Yang Xi , Sun Yuanyuan , Zhu Yanping
Published: March 2023

A practical method was developed for the convenient synthesis of isoxazole-fused tricyclic quinazoline alkaloids. This procedure accesses diverse isoxazole-fused tricyclic quinazoline alkaloids and their derivatives via intramolecular cycloaddition of methyl azaarenes with tert-butyl nitrite (TBN). In this method, TBN acts as the radical initiator and the source of N–O. Moreover, this protocol forms new C–N, C–C, and C–O bonds via sequence nitration and annulation in a one-pot process with broad substrate scope and functionalization of natural products.
An Individuality of Response to Cannabinoids: Challenges in Safety and Efficacy of Cannabis Products
Authors: Kitdumrongthum Sarunya , Trachootham Dunyaporn
Published: March 2023

Since legalization, cannabis/marijuana has been gaining considerable attention as a functional ingredient in food. ∆-9 tetrahydrocannabinol (THC), cannabidiol (CBD), and other cannabinoids are key bioactive compounds with health benefits. The oral consumption of cannabis transports much less hazardous chemicals than smoking. Nevertheless, the response to cannabis is biphasically dose-dependent (hormesis; a low-dose stimulation and a high-dose inhibition) with wide individuality in responses. Thus, the exact same dose and preparation of cannabis may be beneficial for some but toxic to others. The purpose of this review is to highlight the concept of individual variations in response to cannabinoids, which leads to the challenge of establishing standard safe doses of cannabis products for the general population. The mechanisms of actions, acute and chronic toxicities, and factors affecting responses to cannabis products are updated. Based on the literature review, we found that the response to cannabis products depends on exposure factors (delivery route, duration, frequency, and interactions with food and drugs), individual factors (age, sex), and susceptibility factors (genetic polymorphisms of cannabinoid receptor gene, N-acylethanolamine-hydrolyzing enzymes, THC-metabolizing enzymes, and epigenetic regulations). Owing to the individuality of responses, the safest way to use cannabis-containing food products is to start low, go slow, and stay low.
Porous Carbon with Alumina Coating Nanolayer Derived from Biomass and the Enhanced Electrochemical Performance as Stable Anode Materials
Authors: Rehman Wasif ur , Huang Haiming , Yousaf Muhammad Zain , Aslam Farooq , Wang Xueliang , Ghani Awais
Published: March 2023

With the ever-increasing world population, the energy produced from green, environmentally friendly approaches is in high demand. In this work, we proposed a green and cost-effective strategy for synthesizing a porous carbon electrode decorated with alumina oxide (Al2O3) from cherry blossom leaves using the pyrolysis method followed by a sol-gel method. An Al2O3-coating nano-layer (4–6 nm) is formed on the porous carbon during the composition fabrication, which further adversely affects battery performance. The development of a simple rich-shell-structured C@Al2O3 nanocomposite anode is expected to achieve stable electrochemical performances as lithium storage. A significant contributing factor to enhanced performance is the structure of the rich-shell material, which greatly enhances conductivity and stabilizes the solid–electrolyte interface (SEI) film. In the battery test assembled with composite C@Al2O3 electrode, the specific capacity is 516.1 mAh g−1 at a current density of 0.1 A g−1 after 200 cycles. The average discharge capacity of carbon is 290 mAh g−1 at a current density of 1.0 A g−1. The present study proposes bioinspired porous carbon electrode materials for improving the performance of next-generation lithium-ion batteries.
Efficacy of Two Moroccan Cistus Species Extracts against Acne Vulgaris: Phytochemical Profile, Antioxidant, Anti-Inflammatory and Antimicrobial Activities
Authors: Bouabidi Maryem , Salamone Federica Lina , Gadhi Chemseddoha , Bouamama Hafida , Speciale Antonio , Ginestra Giovanna , Pulvirenti Luana , Siracusa Laura , Nostro Antonia , Cristani Mariateresa
Published: March 2023

Background: The genus Cistus L. (Cistaceae) includes several medicinal plants growing wild in the Moroccan area. Acne vulgaris (AV) is a chronic skin disorder treated with topical and systemic therapies that often lead to several side effects in addition to the development of antimicrobial resistance. Our study aimed to investigate the bioactivity of extracts of two Moroccan Cistus species, Cistus laurifolius L. and Cistus salviifolius L., in view of their use as potential coadjuvants in the treatment of mild acne vulgaris. Methods: Targeted phytochemical profiles obtained by HPLC-DAD and HPLC-ESI/MS analyses and biological activities ascertained by several antioxidants in vitro chemical and cell-based assays of the leaf extracts. Moreover, antimicrobial activity against Gram-positive and Gram-negative bacteria, and Candida albicans was evaluated. Results: Analyses revealed the presence of several polyphenols in the studied extracts, mainly flavonoids and tannins. Cistus laurifolius L. and Cistus salviifolius L. possessed good biological properties and all extracts showed antibacterial activity, particularly against Staphylococcus aureus, S. epidermidis, and Propionibacterium acnes, identified as the main acne-causing bacteria. Conclusion: The results suggest that examined extracts are promising agents worthy of further studies to develop coadjuvants/natural remedies for mild acne treatment.
Knowledge Evaluation of Oral Diseases and Perception of Cooperation with Dental Experts for Oral Care of Nurses in Intensive Care Units in Korea: A Preliminary Study
Authors: Kim Yesel , Ku Hye-Min , Jun Mi-Kyoung
Published: March 2023

The aim of this study was to identify the status of education and knowledge concerning oral diseases for oral care as they relate to intensive care unit (ICU) nurses, as well as to investigate the perception of oral care education and practice, as led by dental experts. This study conducted a self-report survey consisting of 33 questions on education and knowledge about oral diseases, as well as perception of dental expert-led education and practice, targeting 240 nurses in the ICU. Finally, 227 questionnaires were analyzed, and 75.3% of the participants were staff nurses, and 41.4% were in the medical ICU. In the area of education and knowledge of major oral diseases, more than 50% of the respondents treating gingivitis, periodontitis, and dental caries did not complete dental education, and it was found that more than half of the respondents were unable to distinguish diseases of the mouth. It was recognized that more than half of nurses required dental expert-led education and practice. In this study, the education and knowledge of oral diseases of ICU nurses were found to be insufficient, and the need for the cooperation of dental experts was high. Therefore, collaboration to improve oral care practical guidelines for realistically applicable ICU patients will be needed.
Spatial Distribution of Drug-Resistant Mycobacterium tuberculosis Infections in Rural Eastern Cape Province of South Africa
Authors: Faye Lindiwe M. , Hosu Mojisola C. , Vasaikar Sandeep , Dippenaar Anzaan , Oostvogels Selien , Warren Rob M. , Apalata Teke
Published: March 2023

Tuberculosis (TB), an infectious airborne disease caused by Mycobacterium tuberculosis (Mtb), is a serious public health threat reported as the leading cause of morbidity and mortality worldwide. South Africa is a high-TB-burden country with TB being the highest infectious disease killer. This study investigated the distribution of Mtb mutations and spoligotypes in rural Eastern Cape Province. The Mtb isolates included were 1157 from DR-TB patients and analysed by LPA followed by spoligotyping of 441 isolates. The distribution of mutations and spoligotypes was done by spatial analysis. The rpoB gene had the highest number of mutations. The distribution of rpoB and katG mutations was more prevalent in four healthcare facilities, inhA mutations were more prevalent in three healthcare facilities, and heteroresistant isolates were more prevalent in five healthcare facilities. The Mtb was genetically diverse with Beijing more prevalent and largely distributed. Spatial analysis and mapping of gene mutations and spoligotypes revealed a better picture of distribution.
Bioactive Antimicrobial Peptides from Food Proteins: Perspectives and Challenges for Controlling Foodborne Pathogens
Authors: Corrêa Jessica Audrey Feijó , de Melo Nazareth Tiago , Rocha Giovanna Fernandes da , Luciano Fernando Bittencourt
Published: March 2023

Bioactive peptides (BAPs) derived from food proteins have been extensively studied for their health benefits, majorly exploring their potential use as nutraceuticals and functional food components. These peptides possess a range of beneficial properties, including antihypertensive, antioxidant, immunomodulatory, and antibacterial activities, and are naturally present within dietary protein sequences. To release food-grade antimicrobial peptides (AMPs), enzymatic protein hydrolysis or microbial fermentation, such as with lactic acid bacteria (LAB), can be employed. The activity of AMPs is influenced by various structural characteristics, including the amino acid composition, three-dimensional conformation, liquid charge, putative domains, and resulting hydrophobicity. This review discusses the synthesis of BAPs and AMPs, their potential for controlling foodborne pathogens, their mechanisms of action, and the challenges and prospects faced by the food industry. BAPs can regulate gut microbiota by promoting the growth of beneficial bacteria or by directly inhibiting pathogenic microorganisms. LAB-promoted hydrolysis of dietary proteins occurs naturally in both the matrix and the gastrointestinal tract. However, several obstacles must be overcome before BAPs can replace antimicrobials in food production. These include the high manufacturing costs of current technologies, limited in vivo and matrix data, and the difficulties associated with standardization and commercial-scale production.
Emergence of Hyper-Epidemic Clones of Enterobacterales Clinical Isolates Co-Producing KPC and Metallo-Beta-Lactamases during the COVID-19 Pandemic
Authors: Faccone Diego , Gomez Sonia A. , de Mendieta Juan Manuel , Sanz María Belén , Echegorry Mariano , Albornoz Ezequiel , Lucero Celeste , Ceriana Paola , Menocal Alejandra , Martino Florencia , De Belder Denise , Corso Alejandra , Pasterán Fernando
Published: March 2023

Background. The global spread of carbapenemase-producing Enterobacterales has become an epidemiological risk for healthcare systems by limiting available antimicrobial treatments. The COVID-19 pandemic worsened this scenario, prompting the emergence of extremely resistant microorganisms. Methods. Between March 2020 and September 2021, the NRL confirmed 82 clinical Enterobacterales isolates harboring a combination of blaKPC and MBL genes. Molecular typing was analyzed by PFGE and MLST. Modified double-disk synergy (MDDS) tests were used for phenotypic studies. Results. Isolates were submitted from 28 hospitals located in seven provinces and Buenos Aires City, including 77 K. pneumoniae, 2 K. oxytoca, 2 C. freundii, and 1 E. coli. Almost half of K. pneumoniae isolates (n = 38; 49.4%), detected in 15 hospitals, belong to the CC307 clone. CC11 was the second clone, including 29 (37.7%) isolates (22, ST11 and 7, ST258) from five cities and 12 hospitals. Three isolates belonging to CC45 were also detected. The carbapenemase combinations observed were as follows: 55% blaKPC-2 plus blaNDM-5; 32.5% blaKPC-2 plus blaNDM-1; 5% blaKPC-3 plus blaNDM-1; 5% blaKPC-2 plus blaIMP-8; and 2.5% strain with blaKPC-2 plus blaNDM-5 plus blaOXA-163. Aztreonam/avibactam and aztreonam/relebactam were the most active combinations (100% and 91% susceptible, respectively), followed by fosfomycin (89%) and tigecycline (84%). Conclusions. The MDDS tests using ceftazidime-avibactam/EDTA and aztreonam/boronic acid disks improved phenotypic classification as dual producers. The successful high-risk clones of K. pneumoniae, such as hyper-epidemic CC307 and CC11 clones, drove the dissemination of double carbapenemase-producing isolates during the COVID-19 pandemic.
Prescription of Rifampicin for Staphylococcus aureus Infections Increased the Incidence of Corynebacterium striatum with Decreased Susceptibility to Rifampicin in a Hungarian Clinical Center
Authors: Orosz László , Lengyel György , Makai Klára , Burián Katalin
Published: March 2023

Several reports have suggested a role for Corynebacterium striatum as an opportunistic pathogen. The authors have conducted a retrospective study at the Clinical Center of the University of Szeged, Hungary, between 2012 and 2021 that revealed significantly increased rifampicin resistance in this species. This work aimed to investigate the reasons behind this phenomenon. The data were collected corresponding to the period between 1 January 2012 and 31 December 2021 at the Department of Medical Microbiology, University of Szeged. To characterize the resistance trends, the antibiotic resistance index was calculated for each antibiotic in use. Fourteen strains with different resistance patterns were further analyzed with Fourier-transform infrared spectroscopy using the IR Biotyper®. The decline in C. striatum sensitivity to rifampicin seen during the COVID-19 pandemic may have been attributable to the use of Rifadin® to treat concomitant Staphylococcus aureus infections. The fact that the IR Biotyper® typing method revealed that the rifampicin-resistant C. striatum strains were closely related supports this hypothesis. The IR Biotyper® infrared spectroscopy proved to be a modern and fast method to support effective antimicrobial stewardship programs.
Effect of Sublethal Concentrations of Zinc Oxide Nanoparticles on Bacillus cereus
Authors: Krzepiłko Anna , Matyszczuk Katarzyna Magdalena , Święciło Agata
Published: March 2023

Zinc oxide nanoparticles (ZnONPs), which are produced on a large scale, pose a potential threat to various environments because they can interact with the microbial populations found in them. Bacteria that are widespread in soil, water, and plant material include the Bacillus cereus group, which plays an important role in biodegradation and the nutrient cycle and is a major factor determining ecological balance. This group includes, among others, the foodborne pathogen B. cereus sensu stricto (herein referred to as B. cereus). The aim of this study was a comprehensive assessment of the effects of commercially available ZnONPs on B. cereus. The MIC (minimum inhibitory concentration) for B. cereus was 1.6 mg/mL, and the MBC (minimum bactericidal concentration) was 1.8 mg/mL. Growth of B. cereus was inhibited by a concentration of ZnONPs lower than or equal to MIC50. Concentrations from 0.2 to 0.8 mg/mL inhibited the growth of these bacteria in liquid media, induced symptoms of oxidative stress, and stimulated an environmental stress response in the form of biofilm and endospore formation. In addition, ZnONPs negatively affected the ability of the bacteria to break down the azo dye Evans Blue but enhanced the antimicrobial properties of phenolic compounds. Sublethal concentrations of ZnONPs generally decreased the activity of B. cereus cells, especially in the presence of phenolics, which indicates their potential toxicological impact, but at the same time they induced universal defence responses in these cells, which in the case of potential pathogens can hinder their removal.
Pre-Professional International Mobility of European Pharmacy Students—A French Example
Authors: Varbanov Mihayl , Bensoussan Danièle , Devocelle Marc
Published: March 2023

Internationalisation, as well as the need to interact with international partners in academia and in the pharmaceutical industry, brings an international experience to the pharmacist’s career, which is essential. The objective of present work is to provide a preliminary study of the current situation of the pre-professional mobility of pharmacy students. It represents the first case study of the international pre-professional mobility of pharmacy students in France, and in north-eastern France in particular. The study is based on a recent preliminary survey among pharmacy students, conducted in 2020 at the University of Lorraine’s Faculty of Pharmacy, reflecting the impact of international mobility programmes, such as the European Union educational and training mobility programme Erasmus+, on the pharmacy curriculum. The results of the present work tend to show that, despite a number of barriers to the international mobility of pharmacy students, the outcomes of international pre-professional mobility are rather positive in their globality.
Precise RF Phase Measurement by Optical Sideband Generation Using Mach–Zehnder Modulators
Authors: Huang Qingchuan , Kawanishi Tetsuya
Published: March 2023

A novel radio frequency (RF) phase measurement method that uses optical modulation was proposed. The mathematical model of Mach–Zehnder modulator (MZM) characterization was reviewed. By measuring the output sideband power of the optical modulator, the phase difference of the electric signals fed to the modulator was experimentally measured, and picosecond-level accuracies were achieved. This technique is expected to be useful for radio-over-fiber-based antenna array systems.
Orbital Angular Momentum Resonances Arising from Mode Coupling in Hollow-Core Fibers
Authors: Alagashev Grigory , Pryamikov Andrey
Published: March 2023

It is known that the growth of the orbital part of the angular momentum of the fundamental air core mode of the negative curvature hollow-core fibers (NCHCFs) with circular polarization occurs at the edge of the transmission bands due to the spin–orbit interaction. In this paper, we consider the resonant behavior of orbital angular momentum (OAM) for a linearly polarized fundamental air core mode in straight and bent NCHCF. The resonant growth of OAM is associated with coupling between the linear polarized fundamental air core mode and the cladding capillary wall modes. The coupling between the modes arises due to crossing and anti-crossing. This OAM growth can be explained by a complex interaction of energy fluxes of the coupled modes under crossing. This phenomenon may be useful for a deeper understanding of the processes arising from mode coupling in fiber optics and nanophotonics, as well as for generating and transmitting OAM modes in micro-structured optical fibers.
Broad Tunable and High-Purity Photonic Microwave Generation Based on an Optically Pumped QD Spin-VCSEL with Optical Feedback
Authors: Shen Zhenye , Huang Yu , Zhu Xin , Zhou Pei , Mu Penghua , Li Nianqiang
Published: March 2023

Spin-polarized vertical-cavity surface-emitting lasers (spin-VCSELs) with birefringence-induced polarization oscillations have been proposed to generate desired photonic microwave signals. Here, we numerically investigate the generation of photonic microwave signals in an optically pumped quantum dot (QD) spin-VCSEL. First, the influence of intrinsic key parameters on period-one (P1) oscillations and microwave properties is discussed. Second, the difference between microwave generation based on the quantum well (QW) and QD spin-VCSELs is analyzed by controlling the carrier capture rate that is described in the spin-flip model. The QD spin-VCSEL shows superior microwave quality in the low-frequency range (e.g., 10 GHz~20 GHz) compared with the QW spin-VCSEL. Finally, to boost the performance of the generated photonic microwave signal, optical feedback is introduced. The results show that dual-loop feedback can simultaneously narrow the microwave linewidth and suppress the side modes that are derived from the external cavity mode.
Resonant Fast-Alfvén Wave Coupling in a 3D Coronal Arcade
Authors: Wright Andrew , Elsden Thomas
Published: March 2023

The resonant excitation of Alfvén waves using the fast magnetosonic mode is important in space plasmas. In this paper, we consider a simple model of a three-dimensional (3D) coronal arcade. A numerical approach is used to produce a driven normal mode. We find that resonant coupling can occur in 3D, but there are new features that are absent in 2D. In particular, the polarisation of the Alfvén waves can vary with position throughout the Resonant Zone. Moreover, there are an infinite number of possible paths the resonant waves can exist on.
Integrative Analysis of Fruit Quality and Anthocyanin Accumulation of Plum cv. ‘Cuihongli’ (Prunus salicina Lindl.) and Its Bud Mutation
Authors: Liao Ling , Li Yaman , Lan Xuejiao , Yang Yiyue , Wei Wen , Ai Jinglan , Feng Xiangning , Chen Hongyu , Tang Yuhang , Xi Lijuan , Wang Zhihui
Published: March 2023

Fruit color is one of the quality indicators to judge the freshness of a plum. The coloring process of plum skin is valuable for research due to the high nutritional quality of anthocyanins found in plums. ‘Cuihongli’ (CHL) and its precocious mutant variety ‘Cuihongli Red’ (CHR) were used to analyze the changes of fruit quality and anthocyanin biosynthesis during plum development. The results showed that, during the development of the two plums, the total soluble solid and soluble sugar contents were highest at the mature stage, as the titratable acid trended gradually downward as the fruits of the two cultivars matured, and the CHR fruit showed higher sugar content and lower acid content. In addition, the skin of CHR turned red in color earlier than CHL. Compared with CHL, the skin of CHR had higher anthocyanin concentrations, higher activities of phenylalanine ammonia-lyase (PAL), chalcone isomerase (CHI), dihydroflavonol-4-reductase (DFR), and UDPglucose: flavonoid-3-O-glucosyltransferase (UFGT), and higher transcript levels of genes associated with anthocyanin production. In the flesh of the two cultivars, no anthocyanin content was detected. Taken together, these results suggest that the mutation exerted a major effect on anthocyanin accumulation via modification of the level of transcription; thus, CHR advances the ripening period of ‘Cuihongli’ plum and improves the fruit quality.