bluuebunny/crossref_metadata_2025 huggingface dataset:
Morphometric Analysis of the Eye by Magnetic Resonance Imaging in MGST2-Gene-Deficient Mice
Authors: Chaomulige  , Matsuo Toshihiko , Sugimoto Kohei , Miyaji Mary , Hosoya Osamu , Ueda Masashi , Kobayashi Ryosuke , Horii Takuro , Hatada Izuho
Published: February 2024

Strabismus, a neuro-ophthalmological condition characterized by misalignment of the eyes, is a common ophthalmic disorder affecting both children and adults. In our previous study, we identified the microsomal glutathione S-transferase 2 (MGST2) gene as one of the potential candidates for comitant strabismus susceptibility in a Japanese population. The MGST2 gene belongs to the membrane-associated protein involved in the generation of pro-inflammatory mediators, and it is also found in the protection against oxidative stress by decreasing the reactivity of oxidized lipids. To look for the roles of the MGST2 gene in the development, eye alignment, and overall morphology of the eye as the possible background of strabismus, MGST2 gene knockout (KO) mice were generated by CRISPR/Cas9-mediated gene editing with guide RNAs targeting the MGST2 exon 2. The ocular morphology of the KO mice was analyzed through high-resolution images obtained by a magnetic resonance imaging (MRI) machine for small animals. The morphometric analyses showed that the height, width, and volume of the eyeballs in MGST2 KO homozygous mice were significantly greater than those of wild-type mice, indicating that the eyes of MGST2 KO homozygous mice were significantly enlarged. There were no significant differences in the axis length and axis angle. These morphological changes may potentially contribute to the development of a subgroup of strabismus.
Calculated Maximal Volume Ventilation (cMVV) as a Marker of Early Respiratory Failure in Amyotrophic Lateral Sclerosis (ALS)
Authors: Manera Umberto , Torrieri Maria Claudia , Moglia Cristina , Canosa Antonio , Vasta Rosario , Palumbo Francesca , Matteoni Enrico , Cabras Sara , Grassano Maurizio , Bombaci Alessandro , Mattei Alessio , Bellocchia Michela , Tabbia Giuseppe , Ribolla Fulvia , Chiò Adriano , Calvo Andrea
Published: February 2024

Respiratory failure assessment is among the most debatable research topics in amyotrophic lateral sclerosis (ALS) clinical research due to the wide heterogeneity of its presentation. Among the different pulmonary function tests (PFTs), maximal voluntary ventilation (MVV) has shown potential utility as a diagnostic and monitoring marker, able to capture early respiratory modification in neuromuscular disorders. In the present study, we explored calculated MVV (cMVV) as a prognostic biomarker in a center-based, retrospective ALS population belonging to the Piemonte and Valle d’Aosta registry for ALS (PARALS). A Spearman’s correlation analysis with clinical data and PFTs showed a good correlation of cMVV with forced vital capacity (FVC) and a moderate correlation with some other features such as bulbar involvement, ALSFRS-R total score, blood oxygen (pO2), carbonate (HCO3−), and base excess (BE), measured with arterial blood gas analysis. Both the Cox proportional hazard models for survival and the time to non-invasive ventilation (NIV) measurement highlighted that cMVV at diagnosis (considering cMVV(40) ≥ 80) is able to stratify patients across different risk levels for death/tracheostomy and NIV indication, especially considering patients with FVC% ≥ 80. In conclusion, cMVV is a useful marker of early respiratory failure in ALS, and is easily derivable from standard PFTs, especially in asymptomatic ALS patients with normal FVC measures.
Competing Visual Cues Revealed by Electroencephalography: Sensitivity to Motion Speed and Direction
Authors: Rassam Rassam , Chen Qi , Gai Yan
Published: February 2024

Motion speed and direction are two fundamental cues for the mammalian visual system. Neurons in various places of the neocortex show tuning properties in term of firing frequency to both speed and direction. The present study applied a 32-channel electroencephalograph (EEG) system to 13 human subjects while they were observing a single object moving with different speeds in various directions from the center of view to the periphery on a computer monitor. Depending on the experimental condition, the subjects were either required to fix their gaze at the center of the monitor while the object was moving or to track the movement with their gaze; eye-tracking glasses were used to ensure that they followed instructions. In each trial, motion speed and direction varied randomly and independently, forming two competing visual features. EEG signal classification was performed for each cue separately (e.g., 11 speed values or 11 directions), regardless of variations in the other cue. Under the eye-fixed condition, multiple subjects showed distinct preferences to motion direction over speed; however, two outliers showed superb sensitivity to speed. Under the eye-tracking condition, in which the EEG signals presumably contained ocular movement signals, all subjects showed predominantly better classification for motion direction. There was a trend that speed and direction were encoded by different electrode sites. Since EEG is a noninvasive and portable approach suitable for brain–computer interfaces (BCIs), this study provides insights on fundamental knowledge of the visual system as well as BCI applications based on visual stimulation.
Norepinephrine-Activated p38 MAPK Pathway Mediates Stress-Induced Cytotoxic Edema of Basolateral Amygdala Astrocytes
Authors: Sun Zhaoling , Zhang Xiaojing , Dong Yiming , Liu Yichang , Wang Chuan , Li Yingmin , Ma Chunling , Xu Guangming , Wang Songjun , Yang Chenteng , Zhang Guozhong , Cong Bin
Published: February 2024

The amygdala is a core region in the limbic system that is highly sensitive to stress. Astrocytes are key players in stress disorders such as anxiety and depression. However, the effects of stress on the morphology and function of amygdala astrocytes and its potential mechanisms remain largely unknown. Hence, we performed in vivo and in vitro experiments using a restraint stress (RS) rat model and stress-induced astrocyte culture, respectively. Our data show that norepinephrine (NE) content increased, cytotoxic edema occurred, and aquaporin-4 (AQP4) expression was up-regulated in the basolateral amygdala (BLA) obtained from RS rats. Additionally, the p38 mitogen-activated protein kinase (MAPK) pathway was also observed to be significantly activated in the BLA of rats subjected to RS. The administration of NE to in vitro astrocytes increased the AQP4 level and induced cell edema. Furthermore, p38 MAPK signaling was activated. The NE inhibitor alpha-methyl-p-tyrosine (AMPT) alleviated cytotoxic edema in astrocytes, inhibited AQP4 expression, and inactivated the p38 MAPK pathway in RS rats. Meanwhile, in the in vitro experiment, the p38 MAPK signaling inhibitor SB203580 reversed NE-induced cytotoxic edema and down-regulated the expression of AQP4 in astrocytes. Briefly, NE-induced activation of the p38 MAPK pathway mediated cytotoxic edema in BLA astrocytes from RS rats. Thus, our data provide novel evidence that NE-induced p38 MAPK pathway activation may be one of the mechanisms leading to cytotoxic edema in BLA under stress conditions, which also could enable the development of an effective therapeutic strategy against cytotoxic edema in BLA under stress and provide new ideas for the treatment of neuropsychiatric diseases.
Glymphatic System Pathology and Neuroinflammation as Two Risk Factors of Neurodegeneration
Authors: Szlufik Stanisław , Kopeć Kamila , Szleszkowski Stanisław , Koziorowski Dariusz
Published: February 2024

The key to the effective treatment of neurodegenerative disorders is a thorough understanding of their pathomechanism. Neurodegeneration and neuroinflammation are mutually propelling brain processes. An impairment of glymphatic system function in neurodegeneration contributes to the progression of pathological processes. The question arises as to how neuroinflammation and the glymphatic system are related. This review highlights the direct and indirect influence of these two seemingly independent processes. Protein aggregates, a characteristic feature of neurodegeneration, are correlated with glymphatic clearance and neuroinflammation. Glial cells cannot be overlooked when considering the neuroinflammatory processes. Astrocytes are essential for the effective functioning of the glymphatic system and play a crucial role in the inflammatory responses in the central nervous system. It is imperative to acknowledge the significance of AQP4, a protein that exhibits a high degree of polarization in astrocytes and is crucial for the functioning of the glymphatic system. AQP4 influences inflammatory processes that have not yet been clearly delineated. Another interesting issue is the gut–brain axis and microbiome, which potentially impact the discussed processes. A discussion of the correlation between the functioning of the glymphatic system and neuroinflammation may contribute to exploring the pathomechanism of neurodegeneration.
Pelvic Fixation Technique Using the Ilio-Sacral Screw for 173 Neuromuscular Scoliosis Patients
Authors: Gaumé Mathilde , Saghbiny Elie , Richard Lou , Thouement Clélia , Vialle Raphaël , Miladi Lotfi
Published: February 2024

Pelvic fixation remains one of the main challenging issues in non-ambulatory neuromuscular scoliosis (NMS) patients, between clinical effectiveness and a high complication rate. The objective of this multicenter and retrospective study was to evaluate the outcomes of a technique that was applied to treat 173 NMS patients. The technique is not well-known but promising; it uses the ilio-sacral screw, combined with either the posterior spinal fusion or fusionless bipolar technique, with a minimum follow-up of two years. The mean operative age of the patients was 13 ± 7 years. The mean preoperative main coronal curve was 64° and improved by a mean of −39° postoperatively. The mean preoperative pelvic obliquity was 23°, which improved by a mean of −14° postoperatively. No decrease in the frontal or sagittal correction was observed during the last follow-up. The sitting posture improved in all cases. Twenty-nine patients (17%) had a postoperative infection: twenty-six were treated with local debridement and antibiotics, and three required hardware removal. Fourteen mechanical complications (8%) occurred: screw malposition (n = 6), skin prominence (n = 1), and connector failure (n = 1). This type of surgery is associated with a high risk for infection. Comorbidities, rather than the surgery itself, were the main risk factors that led to complications. The ilio-sacral screw was reliable and effective in correcting pelvic obliquity in NMS patients. The introduction of intraoperative navigation should minimize the risk of screw misplacement and facilitate revision or primary fixation.
Special Issue “Sports Science in Children”
Authors: Coutinho Diogo , Travassos Bruno , Santos Sara , Figueiredo Pedro , Kelly Adam Leigh
Published: February 2024

In recent times, research and technological advancements have opened an unprecedented window of opportunity for sports science to play a pivotal role in the holistic well-being of children [...]
Neonatal Abstinence Signs during Treatment: Trajectory, Resurgence and Heterogeneity
Authors: Miller Jennifer S. , Bada Henrietta S. , Westgate Philip M. , Sithisarn Thitinart , Leggas Markos
Published: February 2024

Neonatal abstinence syndrome (NAS) presents with a varying severity of withdrawal signs and length of treatment (LOT). We examined the course and relevance of each of the NAS withdrawal signs during treatment in a sample of 182 infants with any prenatal opioid exposure, gestational age ≥ 35 weeks, without other medical conditions, and meeting the criteria for pharmacological treatment. Infants were monitored using the Finnegan Neonatal Abstinence Scoring Tool. Daily mean Finnegan scores were estimated using linear mixed models with random subject effects to account for repeated withdrawal scores from the same subject. Daily item prevalence was estimated using generalized estimating equations with a within-subject exchangeable correlation structure. The median LOT was 12.86 days. The prevalence of withdrawal signs decreased from day one to day three of treatment. However, certain central nervous system (CNS) and gastrointestinal (GI) signs showed sporadic increases in prevalence notable around two weeks of treatment, accounting for increases in Finnegan scores that guided pharmacotherapy. We question whether the resurgence of signs with a prolonged LOT is mainly a consequence of opioid tolerance or withdrawal. Monitoring CNS and GI signs throughout treatment is crucial. Future studies directed to better understand this clinical phenomenon may lead to the refining of NAS pharmacotherapy and perhaps the discovery of treatment alternatives.
Treating Children and Adolescents with Obesity: Predictors of Early Dropout in Pediatric Weight-Management Programs
Authors: Luppino Giovanni , Wasniewska Malgorzata , Casto Celeste , Ferraloro Chiara , Li Pomi Alessandra , Pepe Giorgia , Morabito Letteria Anna , Alibrandi Angela , Corica Domenico , Aversa Tommaso
Published: February 2024

Background: Possible therapeutic failure of pediatric obesity is influenced by the high dropout rate. The aim of this study was to evaluate the rate of dropout and the rate of weight loss over the 24 months of follow-up. Methods: The retrospective, single-center study, involved 489 patients followed for obesity in the period 2016–2020. Patients’ auxological data and blood samples were collected during the first (V1) and last visit (V2). Dropout was defined as a follow-up of less than 12 months and/or including less than one visit every 6 months. Patients were divided into two groups and compared: Group A of dropout (297 patients) and Group B of non-dropout (192 patients). Results: In the follow-up period, which had a mean duration of 24 months, the dropout rate was 60.7%. In Group A, the percentage of patients with BMI ≥ 3 SD at V2 was significantly higher than that in Group B. In Group B, the percentage of patients with pathological HOMA-IR and with fasting glucose >100 mg/dL was higher than group A. The probability of dropout was positively associated with pubertal stage and negatively with impaired fasting glycemia and pathological insulinemia at V1. Conclusion: The study demonstrated a high dropout rate during follow-up, mainly among adolescents and patients with no glucometabolic alterations.
Investigation on Microstructure and Properties of Cold-Sprayed Ni-Mo-Al2O3 Composite Coating
Authors: Gong Yinqing , Xiao Cong , Hu Shunjie , Zhou Yicheng , Li Chenglin , Yang Bing , Zhang Jianqiang , Zhang Guodong
Published: February 2024

In this work, the effect of Mo on the microstructure and properties of Ni-Mo-Al2O3 coatings by cold spraying was studied. The microstructure, composition, hardness, wear resistance and chlorine salt corrosion resistance of the coatings were analyzed by a scanning electron microscope, EDS, X-ray diffractometer, 3D profilometer, microhardness tester and friction wear tester. The results show that the coatings have low porosity and a uniform structure. The addition of Mo can improve the hardness, electrical conductivity, wear resistance and chlorine salt corrosion resistance of the coating but reduce the deposition efficiency of the powder. In general, the 80Ni-10Mo-10Al2O3 coating has the best comprehensive performance, with a hardness of 270.17 HV, friction coefficient of 0.4171 and corrosion rate of 0.287 g/m2·h in molten chloride.
Dynamic Correlations in Disordered Systems: Implications for High-Temperature Superconductivity
Authors: Egami Takeshi
Published: February 2024

Liquids and gases are distinct in their extent of dynamic atomic correlations; in gases, atoms are almost uncorrelated, whereas they are strongly correlated in liquids. This distinction applies also to electronic systems. Fermi liquids are actually gas-like, whereas strongly correlated electrons are liquid-like. Doped Mott insulators share characteristics with supercooled liquids. Such distinctions have important implications for superconductivity. We discuss the nature of dynamic atomic correlations in liquids and a possible effect of strong electron correlations and Bose–Einstein condensation on the high-temperature superconductivity of the cuprates.
Size and Temperature Effects on Band Gap Analysis of a Defective Phononic Crystal Beam
Authors: Yao Bin , Wang Shaopeng , Hong Jun , Gu Shuitao
Published: February 2024

In this paper, a new defective phononic crystal (PC) microbeam model in a thermal environment is developed with the application of modified couple stress theory (MCST). By using Hamilton’s principle, the wave equation and complete boundary conditions of a heated Bernoulli–Euler microbeam are obtained. The band structures of the perfect and defective heated PC microbeams are solved by employing the transfer matrix method and supercell technology. The accuracy of the new model is validated using the finite element model, and the parametric analysis is conducted to examine the influences of size and temperature effects, as well as defect segment length, on the band structures of current microbeams. The results indicate that the size effect induces microstructure hardening, while the increase in temperature has a softening impact, decreasing the band gap frequencies. The inclusion of defect cells leads to the localization of elastic waves. These findings have significant implications for the design of microdevices, including applications in micro-energy harvesters, energy absorbers, and micro-electro-mechanical systems (MEMS).
Effects of Hemiparasites in Grassland Restorations Are Not Universal
Authors: Scheidel Anna , Borowicz Victoria
Published: February 2024

Root hemiparasites infiltrate the vascular tissue of host roots to acquire water and nutrients, which often reduces host growth. Hemiparasites are postulated to be keystone species in grassland communities if they suppress dominant species and increase plant community biodiversity, and ecosystem engineers if they increase nutrient accessibility for surrounding species. We examined keystone effects by evaluating species richness and evenness in 1 m2 plots in a recent prairie restoration where Castilleja sessiliflora was naturally present or absent, and in a longer-established prairie restoration with or without Pedicularis canadensis. We examined ecosystem engineer effects by determining nitrate and phosphate concentrations under, 25 cm from, and 50 cm from hemiparasites, and in the center of hemiparasite-free plots. On the C. sessiliflora site, plots with the hemiparasites had higher species richness due to more forbs and higher floristic quality, consistent with the keystone species hypothesis. Soil phosphate levels were also greater in plots with C. sessiliflora present, consistent with the hypothesis of ecosystem engineering by this hemiparasite. In contrast, plots with/without P. canadensis showed no associations of any community metrics with the hemiparasite, and no correspondence between the presence of hemiparasites and soil nutrients. Although hemiparasites can increase grassland community heterogeneity, the effect is not universal, and the direction and strength of effects likely depends on local conditions.
Ballast-Supported Foundation Designs for Low-Cost Open-Source Solar Photovoltaic Racking
Authors: Vandewetering Nicholas , Jamil Uzair , Pearce Joshua M.
Published: February 2024

Although solar photovoltaic (PV) system costs have declined, capital cost remains a barrier to widespread adoption. Do-it-yourself (DIY) system designs can significantly reduce labor costs, but if they are not attached to a building structure, they require ground penetrating footings. This is not technically and economically feasible at all sites. To overcome these challenges, this study details systems designed to (1) eliminate drilling holes and pouring concrete, (2) propose solutions for both fixed and variable tilt systems, (3) remain cost effective, and (4) allow for modifications to best fit the user’s needs. The ballast-supported foundations are analyzed for eight systems by proposing two separate ballast designs: one for a single line of post systems, and one for a double line of post systems, both built on a 4-kW basis. The results of the analysis found that both designs are slightly more expensive than typical in-ground concrete systems by 25% (assuming rocks are purchased at a landscaping company), but the overall DIY system’s costs remain economically advantageous. Sensitivity analyses are conducted to show how modifications to the dimensions influence the weight of the system and thus change the economic value of the design, so users can trade dimensional freedom for cost savings, and vice versa. Overall, all wood-based PV racking system designs provide users with cost-effective and easy DIY alternatives to conventional metal racking, and the novel ballast systems presented provide more versatility for PV systems installations.
Opinion Formation in the World Trade Network
Authors: Coquidé Célestin , Lages José , Shepelyansky Dima L.
Published: February 2024

We extend the opinion formation approach to probe the world influence of economical organizations. Our opinion formation model mimics a battle between currencies within the international trade network. Based on the United Nations Comtrade database, we construct the world trade network for the years of the last decade from 2010 to 2020. We consider different core groups constituted by countries preferring to trade in a specific currency. We will consider principally two core groups, namely, five Anglo-Saxon countries that prefer to trade in US dollar and the 11 BRICS+ that prefer to trade in a hypothetical currency, hereafter called BRI, pegged to their economies. We determine the trade currency preference of the other countries via a Monte Carlo process depending on the direct transactions between the countries. The results obtained in the frame of this mathematical model show that starting from the year 2014, the majority of the world countries would have preferred to trade in BRI than USD. The Monte Carlo process reaches a steady state with three distinct groups: two groups of countries preferring to trade in whatever is the initial distribution of the trade currency preferences, one in BRI and the other in USD, and a third group of countries swinging as a whole between USD and BRI depending on the initial distribution of the trade currency preferences. We also analyze the battle between three currencies: on one hand, we consider USD, BRI and EUR, the latter currency being pegged by the core group of nine EU countries. We show that the countries preferring EUR are mainly the swing countries obtained in the frame of the two currencies model. On the other hand, we consider USD, CNY (Chinese yuan), OPE, the latter currency being pegged to the major OPEC+ economies for which we try to probe the effective economical influence within international trade. Finally, we present the reduced Google matrix description of the trade relations between the Anglo-Saxon countries and the BRICS+.
On the Dynamic Changes in the Global Stock Markets’ Network during the Russia–Ukraine War
Authors: Zaheer Kashif , Aslam Faheem , Mohmand Yasir Tariq , Ferreira Paulo
Published: February 2024

Analysis of the relationships among global stock markets is crucial for international investors, regulators, and policymakers, particularly during a crisis. Complex network theory was applied to analyze the relationship between global stock markets during the Russia–Ukraine war. Daily data from 55 stock markets from 6 August 2021 to 23 September 2023 were retrieved and used to investigate the changes in global stock market networks. The sample period was divided into 22 subsamples, using a 100-day rolling window rolled forward a trading month, and then long-range correlations based on distance matrices were calculated. These distance matrices were utilized to construct stock market networks. Moreover, minimum spanning trees (MSTs) were extracted from these financial networks for analytical purposes. Based on topological and structural analysis, we identified important/central nodes, distinct communities, vulnerable/stable nodes, and changes thereof with the escalation of war. The empirical findings reveal that the Russia–Ukraine war impacted the global stock markets’ network. However, its intensity varied with changes in the region and the passage of time due to the level of stock market integration and stage of war escalation, respectively. Stock markets of France, Germany, Canada, and Austria remained the most centrally connected within communities; surprisingly, the USA’s stock market is not on this list.
Influence of Blending High-Calcium Additive on Environmental Safety of B, F, and Se: A Case Study from Thermodynamic Calculation
Authors: Sutopo Ulung Muhammad , Desfitri Erda Rahmilaila , Hayakawa Yukio , Kambara Shinji
Published: February 2024

Coal remains an important fuel for use in thermal power plants. However, coal-burning power plants produce large amounts of CFA, which contains TEs such as B, F, and Se, which are leached upon contact with water and act as potential polluters of aquifer systems and soil. To study the transformation of TEs, a thermodynamic calculation (FactSage 7.2) was used. Paper sludge ash was used as a calcium compound additive. The influence of blending a high-calcium additive on the environmental safety of TEs was investigated based on the effect of the mass addition ratio of PS ash. This study’s results confirmed that the leaching processes of TEs, namely (CaO)2(Al2O3), CaF2, Ca10(PO4)F2, and CaSeO4(H2O)2, were caused by the formation of B, F, and Se compounds during the leaching process. Thus, it is clear that calcium has the greatest influence on the transformation of TEs due to their reaction, which, in turn, minimizes the effects of the TEs’ release into the environment. The concentrations of TEs from the sample and addition of PS ash decreased slightly, indicating that the inhibition of TEs was enabled through the addition of PS ash. Although the PS ash YB had the highest calcium content, the PS ash YC gave the best results during the B and Se inhibition processes. The experimental observation was also evaluated for comparison. For the analysis of TEs’ leaching ratios using the thermodynamic calculation and experiment, the experimental results were lower than those initially predicted. These results will help us to choose the best available control technology to minimize the effects of TEs released into the environment.
The Moso Bamboo D-Type Cell Cycle Protein Family: Genome Organization, Phylogeny, and Expression Patterns
Authors: Fang Hui , Mu Changhong , Jiang Jutang , Gao Jian , Cheng Zhanchao
Published: February 2024

Cell cycle proteins and cyclin-dependent kinases (CDKs) play a vital role in the control of cell division, and their complexes form a powerful driving force in pushing cell cycle progression. D-type cyclins (CycDs) are essential for interpreting outside mitogenic signals and regulating the G1 phase. At least 19 distinct CycDs are present in the Moso bamboo (Phyllostachys edulis) genome, belonging to subgroups identified previously in other plants. Silico analysis validated the representative distinctive cyclin domains of each CycD in Moso bamboo, revealing that the genomic architectures of these genes were identical to those of their orthologs in Arabidopsis and rice. Both the phylogeny and covariance suggested that PheCycDs were structurally conserved and had undergone gene duplication. Transcriptome data analysis related to different tissues revealed that most CycDs were highly expressed in Moso bamboo shoots. The addition of growth hormone (NAA) significantly increased the transcript levels of PheCycD4;4, D5;1, D5;2, and D6;1 for a short period of time (6 h), and inhibitors (PCIB) also greatly decreased their expression. These results improved the understanding of PheCycDs in our study, notably in relation to auxin response, and offered an initial insight into the expression pattern and functional mining of the PheCycD gene family.
Individual Tree Species Identification for Complex Coniferous and Broad-Leaved Mixed Forests Based on Deep Learning Combined with UAV LiDAR Data and RGB Images
Authors: Zhong Hao , Zhang Zheyu , Liu Haoran , Wu Jinzhuo , Lin Wenshu
Published: February 2024

Automatic and accurate individual tree species identification is essential for the realization of smart forestry. Although existing studies have used unmanned aerial vehicle (UAV) remote sensing data for individual tree species identification, the effects of different spatial resolutions and combining multi-source remote sensing data for automatic individual tree species identification using deep learning methods still require further exploration, especially in complex forest conditions. Therefore, this study proposed an improved YOLOv8 model for individual tree species identification using multisource remote sensing data under complex forest stand conditions. Firstly, the RGB and LiDAR data of natural coniferous and broad-leaved mixed forests under complex conditions in Northeast China were acquired via a UAV. Then, different spatial resolutions, scales, and band combinations of multisource remote sensing data were explored, based on the YOLOv8 model for tree species identification. Subsequently, the Attention Multi-level Fusion (AMF) Gather-and-Distribute (GD) YOLOv8 model was proposed, according to the characteristics of the multisource remote sensing forest data, in which the two branches of the AMF Net backbone were able to extract and fuse features from multisource remote sensing data sources separately. Meanwhile, the GD mechanism was introduced into the neck of the model, in order to fully utilize the extracted features of the main trunk and complete the identification of eight individual tree species in the study area. The results showed that the YOLOv8x model based on RGB images combined with current mainstream object detection algorithms achieved the highest mAP of 75.3%. When the spatial resolution was within 8 cm, the accuracy of individual tree species identification exhibited only a slight variation. However, the accuracy decreased significantly with the decrease of spatial resolution when the resolution was greater than 15 cm. The identification results of different YOLOv8 scales showed that x, l, and m scales could exhibit higher accuracy compared with other scales. The DGB and PCA-D band combinations were superior to other band combinations for individual tree identification, with mAP of 75.5% and 76.2%, respectively. The proposed AMF GD YOLOv8 model had a more significant improvement in tree species identification accuracy than a single remote sensing sources and band combinations data, with a mAP of 81.0%. The study results clarified the impact of spatial resolution on individual tree species identification and demonstrated the excellent performance of the proposed AMF GD YOLOv8 model in individual tree species identification, which provides a new solution and technical reference for forestry resource investigation combined multisource remote sensing data.
Development of a Tool for Navigating the Evidence concerning Land Managers and Woodland Creation in the United Kingdom
Authors: Petrokofsky Gillian , Dunn Michael , Edwards David , Harvey William J. , Hemery Gabriel E. , Marshall Harry , McConnachie Stephen , Petrokofsky Leo , Watts Kevin , Ambrose-Oji Bianca
Published: February 2024

Woodland creation has become an important objective for a variety of stakeholders to help tackle the climate and biodiversity crises. One of the key evidence needs is a better understanding of the multiple factors influencing the willingness and ability of landowners and managers to establish new woodlands. To address this gap, a systematic map of evidence was prepared comprising publications from academic journals and grey literature accessed through bibliographic databases (Web of Science, Scopus, and CAB Abstracts), libraries, direct requests to relevant organisations and individuals, and citation tracking from past reviews. A screening process refined the evidence base to 226 studies within the UK. The systematic evidence map codes the content of each study against a comprehensive list comprising actors, drivers of or barriers to woodland creation, and outcomes. These are presented as a freely accessible, interactive online dashboard detailing sources of evidence. The systematic evidence map helps users navigate the evidence, demonstrating where the bulk of the evidence lies and, conversely, several evidence gaps where there is comparatively little evidence. The findings serve as a basis for dialogue with stakeholders to determine priorities for future primary research.
A Methodological Framework for Decomposing the Value-Chain Economic Contribution: A Case of Forest Resource Industries of the Lake States in the United States
Authors: Gc Shivan , Thapa Ichchha , Pokharel Raju , Alward Greg , Lamsal Basanta , Poudel Jagdish , Dahal Ram , Joshi Omkar , Parajuli Rajan , Wagner John , Leefers Larry
Published: February 2024

The forest products industries play a vital role in the economic, social, and environmental well-being of the Lake States in the United States. While various economic contribution analyses of forest products industries have been conducted to highlight the importance of such industries to regional economies, little effort has yet been made to parse out the contribution of activities in the value chain. The value chain is a series of steps involved in producing goods or services. This study used a matrix decomposition approach to estimate the economic contribution along the value chain through multiple pathways of four forest resource-based industries using wood as inputs: biomass power generation, sawmills, paper mills, and the construction of new single-family residential structures in the Lake States. The direct and indirect economic output values in 2017 resulting from the construction of new single-family residential structures were $19.1 billion, sawmills were $2.5 billion, paper mills were $17.6 billion, and the biomass power generation industry was $759 million. Of the direct and indirect economic output contributed by each industry, the highest percentage of output attributable to the logging industry was observed from the sawmills industry (12%), followed by biomass power generation (9%), paper mills (1.4%), and the construction of new single-family residential structures (<1%), respectively. The percentage of total economic output attributable to the stumpage industry in the region followed a similar trend as commercial logging for all value-chain industries. The relative economic contribution of the value-chain industries to the total economic contribution of the final industry varied based on whether the industry was a primary or secondary forest products industry and the pathways used for sourcing wood inputs.
Allium sativum L. var. Voghiera Reduces Aflatoxin B1 Bioaccessibility and Cytotoxicity In Vitro
Authors: Lázaro Álvaro , Frangiamone Massimo , Maietti Annalisa , Cimbalo Alessandra , Vila-Donat Pilar , Manyes Lara
Published: February 2024

The present work focuses on the evaluation of AFB1′s bioaccessibility and cytotoxicity in vitro using bread (naturally contaminated) enriched or not enriched with fresh Voghiera garlic (2%). Two different experiments were carried out: experiment 1 (E1), with low-AFB1-concentration breads (1.6–1.7 mg/kg); and experiment 2 (E2), with high-AFB1-concentration breads (96.4–102.7 mg/kg). Eight breads were prepared, four for E1 (experiment 1) and another four for E2 (experiment 2), with each experiment having a control group (C), a garlic-enriched group (2%) (G), an AFB1 group (A), and an AFB1 + garlic group (A + G). Simulated digestion was performed on each type of bread, and gastric and intestinal digests were obtained. AFB1 content in flours, baked bread, and gastric and intestinal digests was measured by High-Performance Liquid Chromatography coupled to Fluorescence Detection. The results demonstrate dose-dependent AFB1 bioaccessibility and that the presence of garlic contributed to its reduction in both doses (7–8%). Moreover, garlic’s presence in AFB1-contaminated bread increased cell viability (9–18%) in differentiated Caco-2 cells and mitigated the arrest of S and G2/M phases provoked by AFB1 on Jurkat T cells and reduced apoptosis/necrosis, cellular reactive oxygen species (ROS), and mitochondrial ROS by 16%, 71%, and 24% respectively. The inclusion of garlic as a functional ingredient helped relieve the presence and effects of AFB1.
The Prebiotic Diet: Other Dietary Molecules Implicated in Gut Microbiota Health
Authors: Rinninella Emanuele , Costantini Lara
Published: February 2024

In 2016, the International Scientific Association for Probiotics and Prebiotics (ISAPP) provided a new definition of a prebiotic as “a substrate that is selectively utilized by host microorganisms conferring a health benefit” [...]
Effects of Phenolic Evolution on Color Characteristics of Single-Cultivar Vitis vinifera L. Marselan and Merlot Wines during Vinification and Aging
Authors: Zhang Hua-Lin , Xia Nong-Yu , Yao Xue-Chen , Duan Chang-Qing , Pan Qiu-Hong
Published: February 2024

The loss of red hue in dry red wine has been a persistent issue for wine enterprises in western China. We investigated the changes in anthocyanins and non-anthocyanin phenols during the industrial-scale fermentation and one-year bottle aging of Vitis vinifera L. Merlot and Vitis vinifera L. Marselan, respectively, using the grapes in the Ningxia region. We also examined their correlation with color characterization. The study found that both anthocyanins and non-anthocyanin phenolics were rapidly extracted from grapes during alcohol fermentation. However, their concentrations decreased rapidly during malolactic fermentation. On the other hand, Vitisin A and Vitisin B were formed during alcoholic fermentation and decreased slowly from malolactic fermentation to storage period. Directly polymerized pigments (F-A and A-F), bridged polymerized pigments (A-e-F), and flavanyl-pyranoanthocyanins (A-v-F) from the reactions of anthocyanins (A) and flavan-3-ols (F), as well as pinotins were generated during the later stages of alcoholic fermentation, and remained at a high level throughout malolactic fermentation and bottle storage. Partial least squares regression and Pearson correlation analyses revealed that the red hue (a* value) of ‘Merlot’ and ‘Marselan’ wines was closely associated with monomeric anthocyanins and F-A type pigments. Furthermore, four pinotin components were positively correlated with the red hue (a* value) of ‘Merlot’ wine. These primary red components of the two varieties had a positive correlation with the level of flavan-3-ols. The data suggest that elevating the flavan-3-ol concentration during fermentation aids in improving the color stability of red wine.
Optimizing Quality and Shelf-Life Extension of Bor-Thekera (Garcinia pedunculata) Juice: A Thermosonication Approach with Artificial Neural Network Modeling
Authors: Gogoi Shikhapriyom , Das Puja , Nayak Prakash Kumar , Sridhar Kandi , Sharma Minaxi , Sari Thachappully Prabhat , Kesavan Radha krishnan , Bhaswant Maharshi
Published: February 2024

This study investigated the quality characteristics of pasteurized and thermosonicated bor-thekera (Garcinia pedunculata) juices (TSBTJs) during storage at 4 °C for 30 days. Various parameters, including pH, titratable acidity (TA), total soluble content (TSSs), antioxidant activity (AA), total phenolic content (TPC), total flavonoid content (TFC), ascorbic acid content (AAC), cloudiness (CI) and browning indexes (BI), and microbial activity, were analyzed at regular intervals and compared with the quality parameters of fresh bor-thekera juice (FBTJ). A multi-layer artificial neural network (ANN) was employed to model and optimize the ultrasound-assisted extraction of bor-thekera juice. The impacts of storage time, treatment time, and treatment temperature on the quality attributes were also explored. The TSBTJ demonstrated the maximum retention of nutritional attributes compared with the pasteurized bor-thekera juice (PBTJ). Additionally, the TSBTJ exhibited satisfactory results for microbiological activity, while the PBTJ showed the highest level of microbial inactivation. The designed ANN exhibited low mean squared error values and high R2 values for the training, testing, validation, and overall datasets, indicating a strong relationship between the actual and predicted results. The optimal extraction parameters generated by the ANN included a treatment time of 30 min, a frequency of 44 kHz, and a temperature of 40 °C. In conclusion, thermosonicated juices, particularly the TSBTJ, demonstrated enhanced nutritional characteristics, positioning them as valuable reservoirs of bioactive components suitable for incorporation in the food and pharmaceutical industries. The study underscores the efficacy of ANN as a predictive tool for assessing bor-thekera juice extraction efficiency. Moreover, the use of thermosonication emerged as a promising alternative to traditional thermal pasteurization methods for bor-thekera juice preservation, mitigating quality deterioration while augmenting the functional attributes of the juice.
Chitosan and Sodium Hyaluronate Hydrogels Supplemented with Bioglass for Bone Tissue Engineering
Authors: Ciołek Lidia , Zaczyńska Ewa , Krok-Borkowicz Małgorzata , Biernat Monika , Pamuła Elżbieta
Published: February 2024

The aim of the study was to produce biocomposites based on chitosan and sodium hyaluronate hydrogels supplemented with bioglasses obtained under different conditions (temperature, time) and to perform an in vitro evaluation of their cytocompatibility using both indirect and direct methods. Furthermore, the release of ions from the composites and the microstructure of the biocomposites before and after incubation in simulated body fluid were assessed. Tests on extracts from bioglasses and hydrogel biocomposites were performed on A549 epithelial cells, while MG63 osteoblast-like cells were tested in direct contact with the developed biomaterials. The immune response induced by the biomaterials was also evaluated. The experiments were carried out on both unstimulated and lipopolysaccharide (LPS) endotoxin-stimulated human peripheral blood cells in the presence of extracts of the biocomposites and their components. Extracts of the materials produced do not exhibit toxic effects on A549 cells, and do not increase the production of proinflammatory cytokines tumour necrosis factor alpha (TNF-α) and interleukin (IL-6) by blood cells in vitro. In direct contact with MG63 osteoblast-like cells, biocomposites containing the reference bioglass and those containing SrO are more cytocompatible than biocomposites with ZnO-doped bioglass. Using two testing approaches, the effects both of the potentially toxic agents released and of the surface of the tested materials on the cell condition were assessed. The results pave the way for the development of highly porous hydrogel–bioglass composite scaffolds for bone tissue engineering.
“Family Trouble”: The 1975 Killing of Denise Hawkins and the Legacy of Deadly Force in the Rochester, NY Police Department
Authors: Forsyth Ted , Szymanski Mallory
Published: February 2024

This paper examines the lineages of police violence, family trauma, and police reform through a case study of the Rochester police killing of Denise Hawkins in 1975. Michael Leach, a 22-year-old, white police officer, responded to a “family trouble” call involving a domestic dispute between Hawkins and her husband. When the 18-year-old, 100-pound Black woman emerged from the apartment, she held a kitchen knife. Within five seconds, Leach had shot and killed her, later claiming she endangered his life. Though Hawkins’ name is included in lists of Black women killed by police, little is known about her life and legacy. Using newspapers, police records, and oral history, we examine activists’ attempts to scale the call for justice for Denise Hawkins to the national level, the police department’s defense of Leach as the true victim in the incident, and the city leaders’ compromised efforts to establish a civilian oversight of police. Within the context of Rochester’s robust history of resistance to police violence, we argue that the reform efforts of the late 1970s ultimately failed to redress the police use of deadly force. Furthermore, when Michael Leach killed again in 2012—this time shooting his own son, whom he mistook for an intruder—his defense attorney successfully depicted Leach as the sympathetic figure. In shifting the focus to Denise Hawkins, this work contributes to the Black feminist call to memorialize Black women killed by police and suggests that the policies that protect the officers who use deadly force cause widespread, intergenerational harm to officers and their victims.
Somatic Mutations within Myocilin due to Aging May Be a Potential Risk Factor for Glaucoma
Authors: Sazhnyev Yevgeniy , Venkat Akaash , Zheng Jie J.
Published: February 2024

Glaucoma is a chronic optic neuropathy that leads to irreversible vision loss. Aging and family history are the two most important risk factors of glaucoma. One of the most studied genes involved in the onset of open-angle glaucoma is myocilin (MYOC). About 105 germline mutations within MYOC are known to be associated with glaucoma and result in endoplasmic reticulum (ER) stress, which leads to trabecular meshwork (TM) cell death and subsequent intraocular pressure (IOP) elevation. However, only about 4% of the population carry these mutations. An analysis of MYOC somatic cancer-associated mutations revealed a notable overlap with pathogenic glaucoma variants. Because TM cells have the potential to accumulate somatic mutations at a rapid rate due to ultraviolet (UV) light exposure, we propose that an accumulation of somatic mutations within MYOC is an important contributor to the onset of glaucoma.
A Longitudinal Study on Cognitive Training for Cognitively Preserved Adults in Liguria, Italy
Authors: Veneziano Massimo , Piazza Maria Francesca , Palummeri Ernesto , Paganino Chiara , Andreoli Giovanni Battista , Amicizia Daniela , Ansaldi Filippo
Published: February 2024

In this study, we examined the effects of memory training on cognitive function and depressive symptoms in a cohort of 794 healthy adults aged 50 years or older. Participants were divided into an active intervention group and a passive intervention group, with various cognitive measures assessed over a one-year period. Univariate analysis revealed that the active intervention group consistently outperformed the passive group in measures of memory self-perception (Memory Complaint Questionnaire—MACQ), depressive symptoms (Geriatric Depression Scale—GDS-4), verbal memory and recall ability (A3LP), and verbal fluency (VF). Significant differences in MACQ scores were observed between the two groups at all time points, indicating enhanced memory self-perception in the active group. GDS-4 scores consistently favored the active group, suggesting a reduction in depressive symptoms. A3LP scores demonstrated that the active group had better verbal memory and recall abilities. VF scores consistently favored the active group, indicating superior language skills and cognitive flexibility. Linear regression model and mixed linear regression model reinforced these findings, with highly significant interaction effects observed between the active/passive group, gender, age, education, and time. These effects were particularly pronounced for MACQ and A3LP scores, indicating the combined impact of these factors on memory self-perception and verbal memory. This study highlights the positive impact of memory training intervention on cognitive function and depressive symptoms in older adults and underscores the importance of considering gender, age, and education in cognitive interventions. Notably, these benefits persist for up to six months from the end of the program. The results provide valuable insights into cognitive changes in aging populations and suggest that tailored memory training programs can yield significant improvements.