bluuebunny/crossref_metadata_2025 huggingface dataset:
Copper Chaperone for Superoxide Dismutase FoCCS1 in Frankliniella occidentalis May Be Associated with Feeding Adaptation after Host Shifting
Authors: Zhang Tao , Liu Li , Zhi Jun-Rui , Jia Yu-Lian , Yue Wen-Bo , Zeng Guang , Li Ding-Yin
Published: August 2022

Western flower thrips (Frankliniella occidentalis) pose a serious threat to the global vegetable and flower crop production. The regulatory mechanism for superoxide dismutase (SOD) in the feeding adaptation of F. occidentalis after host shifting remains unclear. In this study, the copper chaperone for SOD (CCS) and manganese SOD (MnSOD) genes in F. occidentalis were cloned, and their expression levels at different developmental stages was determined. The mRNA expression of FoCCS1 and FoMnSOD2 in F. occidentalis second-instar larvae and adult females of F1, F2, and F3 generations was analyzed after shifting the thrips to kidney bean and broad bean plants, respectively. The F2 and F3 second-instar larvae and F2 adult females showed significantly upregulated FoCCS1 mRNA expression after shifting to kidney bean plants. The F1 second-instar larvae and F2 adult females showed significantly upregulated FoCCS1 mRNA expression after shifting to broad bean plants. The RNA interference significantly downregulated the FoCCS1 mRNA expression levels and adult females showed significantly inhibited SOD activity after shifting to kidney bean and broad bean plants. F. occidentalis adult females subjected to RNA interference and released on kidney bean and broad bean leaves for rearing, respectively, significantly reduced the survival rate and fecundity. These findings suggest that FoCCS1 plays an active role in regulating the feeding adaptation ability of F. occidentalis after host shifting.
Energy Reconstruction and Calibration of the MicroBooNE LArTPC
Authors: Diurba Richard
Published: August 2022

MicroBooNE uses a liquid argon time projection chamber (LArTPC) for simultaneous tracking and calorimetry. Neutrino oscillation experiments plan to use LArTPCs over the next several decades. A challenge for these current and future experiments lies in characterizing detector performance and reconstruction capabilities with thorough associated systematic uncertainties. This work includes updates related to LArTPC detector physics challenges by reviewing MicroBooNE’s recent publications on calorimetry and its applications. Highlights include discussions on signal processing, calorimetric calibration, and particle identification.
Growth Factor Release within Liquid and Solid PRF
Authors: Zwittnig Katharina , Kirnbauer Barbara , Jakse Norbert , Schlenke Peter , Mischak Irene , Ghanaati Shahram , Al-Maawi Sarah , Végh Dániel , Payer Michael , Zrnc Tomislav A.
Published: August 2022

Aim: The purpose of this study was to obtain data concerning growth factor release within liquid and solid platelet-rich fibrin (PRF) matrices and to estimate the amount of potential interindividual variations as a basis for further preclinical and clinical trials. Therefore, we aimed to determine possible differences in the release of growth factors between liquid and solid PRF. Materials and Methods: Blood samples obtained from four subjects were processed to both liquid and solid PRF matrices using a standard centrifugation protocol. Five growth factors (vascular endothelial growth factor, VEGF; epidermal growth factor, EGF; platelet-derived growth factor-BB, PDGF-BB; transforming growth factor-β1, TGF-β1; and matrix metallopeptidase 9, MMP-9) have been evaluated at six time points by ELISA over a total observation period of 10 days (1 h, 7 h, 1 d, 2 d, 7 d, and 10 d). Results: Growth factor release could be measured in all samples at each time point. Comparing liquid and solid PRF matrices, no significant differences were detected (p > 0.05). The mean release of VEGF, TGFβ-1, PDGF-BB, and MMP-9 raised to a peak at time point five (day 7) in both liquid and solid PRF matrices. VEGF release was lower in liquid PRF than in solid PRF, whereas those of PDGF-BB and MMP-9 were higher in liquid PRF than in solid PRF at all time points. EGF had its peak release already at time point two after 7 h in liquid and solid matrices (hour 7 EGF solid: mean = 180 pg/mL, SD = 81; EGF liquid: mean = 218 pg/mL, SD = 64), declined rapidly until day 2, and had a second slight peak on day 7 in both groups (day 7 EGF solid: mean = 182 pg/mL, SD = 189; EGF liquid: mean = 81 pg/mL, SD = 70). Conclusions: This study detected growth factor release within liquid and solid PRF matrices with little variations. Further preclinical trials are needed to precisely analyze the growth factor release in larger samples and to better understand their effects on wound healing in different clinical indications.
Tibial Tubercle to Trochlear Groove Distance Measured by Posterior Condylar Reference Line on MRI Can Over-Evaluate Lateralization Deformity of Tibial Tubercle in Patients with Recurrent Patellar Dislocation
Authors: Zhao Pei , Chen Jiaxing , Feng Yi , Tan Hao , Yin Baoshan , Zhang Hua , Zhang Jian , Zhou Aiguo
Published: August 2022

Background: The tibial tubercle to trochlear groove (TT-TG) distance is currently considered as an indication for tibial tubercle osteotomy. While the influence of femoral condylar morphology on such measurement remains unclear. Methods: A total of 86 patients with patellar dislocation (PD) and 86 healthy individuals were enrolled. Femoral condylar morphology and the TT-TG distance measured by anatomical transepicondylar axis (TT-TGa), by surgical transepicondylar axis (TT-TGs), and by posterior condylar reference line (TT-TGp) were assessed by MRI. Unpaired t-test, Spearman, and Pearson correlation analysis were conducted. We determined the pathological value of the parameters and established a binary regression model. Results: The interclass correlation coefficients of all the TT-TG distances were greater than 0.75 in all types of trochlear dysplasia. The lateral/posterior femoral condyle was shorter and the medial/posterior condyle was longer in the study group (28.5 ± 3.3 and 35.2 ± 2.8, respectively) than in the control group (30.9 ± 2.7 and 33.5 ± 2.3, respectively). In the study group, the TT-TGp distance was greater than TT-TGs and TT-TGa distance (p < 0.001). The pathological value of the TT-TG distance was 13.0 mm. Each TT-TG distance revealed a significant OR with regard to PD. Conclusion: The TT-TGa, TT-TGs, and TT-TGp distance can be reliably measured by MRI even in patients with trochlear dysplasia. While the TT-TGp distance may overestimate the lateralization deformity of the tibial tubercle. Posterior femoral condylar dysplasia may be a reason for such overestimating. These findings have not been correlated to clinical outcomes and further studies are required.
Potential Usefulness of Blood Urea Nitrogen to Creatinine Ratio in the Prediction and Early Detection of Delirium Motor Subtype in the Intensive Care Unit
Authors: Park Woo Rhim , Kim Hye Rim , Park Jin Young , Kim Hesun Erin , Cho Jaehwa , Oh Jooyoung
Published: August 2022

Prediction and early detection of delirium can improve patient outcomes. A high blood urea nitrogen to creatinine ratio (BCR), which reflects dehydration, has been reported as a risk factor for delirium. Additionally, BCR represents skeletal muscle loss in intensive care unit (ICU) patients, which can have critical implications for clinical outcomes. We investigated whether BCR could be used to predict the occurrence and motor subtype of delirium in ICU patients through a retrospective cohort study that included 7167 patients (50 years or older) admitted to the ICU. Patients were assessed daily using the Richmond Agitation-Sedation Scale and the Confusion Assessment Method for ICU and categorized according to the delirium subtype. Participants were split into 10 groups according to BCR at ICU admission and the prevalence of each delirium subtype was compared. Multivariable logistic regression was then used for analysis. A higher BCR at ICU admission was associated with the development of hypoactive delirium. Moreover, BCR > 24.9 was associated with higher rates of hypoactive delirium. Our findings showed that a high BCR at ICU admission was associated with the development of hypoactive delirium, which suggested that BCR could be a potential biomarker for hypoactive delirium in ICU patients.
Antimicrobial Peptides in Early-Life Host Defense, Perinatal Infections, and Necrotizing Enterocolitis—An Update
Authors: Agakidou Eleni , Agakidis Charalampos , Kontou Angeliki , Chotas William , Sarafidis Kosmas
Published: August 2022

Host defense against early-life infections such as chorioamnionitis, neonatal sepsis, or necrotizing enterocolitis (NEC) relies primarily on innate immunity, in which antimicrobial peptides (AMPs) play a major role. AMPs that are important for the fetus and neonate include α and β defensins, cathelicidin LL-37, antiproteases (elafin, SLPI), and hepcidin. They can be produced by the fetus or neonate, the placenta, chorioamniotic membranes, recruited neutrophils, and milk-protein ingestion or proteolysis. They possess antimicrobial, immunomodulating, inflammation-regulating, and tissue-repairing properties. AMPs are expressed as early as the 13th week and increase progressively through gestation. Limited studies are available on AMP expression and levels in the fetus and neonate. Nevertheless, existing evidence supports the role of AMPs in pathogenesis of chorioamnionitis, neonatal sepsis, and NEC, and their association with disease severity. This suggests a potential role of AMPs in diagnosis, prevention, prognosis, and treatment of sepsis and NEC. Herein, we present an overview of the antimicrobial and immunomodulating properties of human AMPs, their sources in the intrauterine environment, fetus, and neonate, and their changes during pre- and post-natal infections and NEC. We also discuss emerging data regarding the potential utility of AMPs in early-life infections, as diagnostic or predictive biomarkers and as therapeutic alternatives or adjuncts to antibiotic therapy considering the increase of antibiotic resistance in neonatal intensive care units.
A Novel Propofol Dosing Regimen for Pediatric Sedation during Radiologic Tests
Authors: Min Ji-Young , Lee Jeong-Rim , Lee Hye-Mi , Nam Ho-Jae , Byon Hyo-Jin
Published: August 2022

The dose of propofol for pediatric sedation during radiologic tests has been proposed as an equation of 0.75 + 0.14 × age (months) + 45.82 × body surface area (m2) based on results in a previous study. We compared this equation and the conventional dosing strategy for sedation in children undergoing radiologic tests. An amount of 180 children scheduled for magnetic resonance imaging (MRI) were randomized to experimental and control groups. The initial induction dose of propofol calculated using the equation was administered in the experimental group. In the control group, children received 1 mg/kg of the initial induction dose of propofol. Then, 0.5 mg/kg of the additional dose was followed to induce sedation in both groups. When awake or moving, a rescue injection of 0.5 mg/kg propofol was given. The total induction dose was more significant in the experimental group. The number of injections for induction in the experimental group was lesser. The dose and number of rescue injections in the experimental group were significantly less. The equation for the induction dose of propofol in a previous study could achieve quick induction of sedation and prevent a rescue injection during sedation. However, caution is needed when using the equation.
Neurophysiological Correlates in Patients with Syringomyelia and Chiari Malformation: The Cortico-Diaphragmatic Involvement
Authors: Cocito Dario , Peci Erdita , Garbossa Diego , Ciaramitaro Palma
Published: August 2022

Purpose. Brainstem syndromes have frequently been reported in Chiari syndrome and in syringobulbia; previous studies have shown that determining the central motor conduction time (CMCT) along the circuit of the phrenic nerve makes the assessment of the voluntary control of the respiratory pathway possible. In our study, we evaluated the transcranial magnetic stimulation (TMS) of the phrenic nerve in patients affected by Chiari syndrome and/or syringomyelia (Syr) with the aim of identifying subclinical neurophysiological alterations. Methods. One hundred patients (75 females; average age: 51 ± 13.08 SD; range: 18–76) affected by Chiari syndrome and/or Syr without dyspnea were selected. The magnetic stimulation of the second motor neuron correlating with the phrenic nerve was performed using cervical magnetic stimulation (C5-MEP); the cortical MEP after magnetic stimulation (Cz-MEP) was recorded by magnetic stimulation of the motor cortex (areas corresponding to the diaphragm). The CMCT was calculated. The differences between the patients and controls were calculated (Student’s t test). Results. In 13% of the patients, the Cz-MEP were absent bilaterally, and the CMCT was not evaluable. In all these cases, bulbar/cervical Syr was present at MRI; in 10 of them, this was associated with CM1. A bilateral response was obtained in all the other patients (87%), and the CMCTs were normal. All the patients with alterations/absence of Cz-MEP presented bulbar/cervical Syr at MRI. The C5-MEP latency was prolonged or absent in 48%; of these, 84% presented bulbar/cervical Syr associated with CM1 at MRI. The C5-MEP latency values were significantly higher in the group of patients. Conclusions. Neurophysiological alterations of the diaphragmatic pathway were recorded in a group of Chiari syndrome and, particularly, in bulbar/cervical Syr. Future studies with larger cohorts of patients are needed to further assess the specific role of the TMS of the phrenic nerve in CM/Syr patients.
Toxicological and Biochemical Description of Synergism of Beauveria bassiana and Emamectin Benzoate against Megalurothrips usitatus (Bagrall)
Authors: Zhang Youdan , Zhang Xiaochen , Tian Qingheng , Ali Shaukat , Tang Liangde , Wu Jianhui
Published: August 2022

The prophylactic application of synthetic insecticides to manage Megalurothrips usitatus (Bagrall) has resulted in insecticide resistance and negative impacts upon natural ecosystems. This has driven the need for developing alternative pest control strategies. In the present study, we investigated the synergistic interaction between the entomopathogenic fungus Beauveria bassiana and the insecticide emamectin benzoate on M. usitatus. The results of our research exhibited that higher doses of emamectin benzoate inhibited the germination rate and colony growth of B. bassiana. The percentage of M. usitatus mortality following B. bassiana and emamectin benzoate treatment indicated a dose–mortality effect. All concentrations of emamectin benzoate combined with different concentrations of B. bassiana demonstrated a synergistic effect five days post-treatment. When B. bassiana and emamectin benzoate were applied alone or in combination, antioxidant enzyme activities, including acetylcholinesterase, catalase, superoxide dismutase, and peroxidase, were significantly lower in M. usiatus than in the controls at the end of the experimental period. The findings of our study confirm the synergistic effect of B. bassiana and emamectin benzoate on M. usitatus, as well as the biochemical process that might be involved in the regulation of the synergistic effect.
Investigating the Effect of Alcohol Dehydrogenase Gene Knockout on Lipid Accumulation in Mucor circinelloides WJ11
Authors: Shah Aabid Manzoor , Mohamed Hassan , Fazili Abu Bakr Ahmad , Yang Wu , Song Yuanda
Published: August 2022

Mucor circinelloides is an oleaginous, dimorphic zygomycete fungus species that produces appreciable levels of ethanol when grown under aerobic conditions in the presence of high glucose, indicating the fungus is a Crabtree-positive microorganism. Engineering efforts to redirect carbon flux from ethanol to lipid biosynthesis may shed light on the critical role of ethanol biosynthesis during aerobic fermentation in M. circinelloides. Therefore, in this study, the alcohol dehydrogenase gene (ADH1) of M. circinelloides WJ11 was deleted, and its effects on growth, lipid production, and fatty acid content were analyzed. Our results showed that knocking out of adh1∆ reduced the ethanol concentration by 85–90% in fermented broth, indicating that this gene is the major source of ethanol production. Parallel to these findings, the lipid and fatty acid content of the mutant was decreased, while less change in the growth of WJ11 was observed. Furthermore, a fermentation study showed the lipid and fatty acid content was restored in the mutant strain when the fermentation media was supplemented with 0.5% external ethanol, indicating the importance of alcohol dehydrogenase and its product on growth and lipid biosynthesis in M. circinelloides. To our knowledge, this is the first study to show a link between alcohol dehydrogenase and lipid production in M. circinelloides.
Differential Activity of the Extracellular Phenoloxidases in Different Strains of the Phytopathogenic Fungus, Microdochium nivale
Authors: Vetchinkina Elena , Meshcherov Azat , Gorshkov Vladimir
Published: August 2022

To cause plant diseases, phytopathogenic fungi use numerous extracellular enzymes, among which, the phenoloxidases (POs) seem underestimated for the pathogens of non-woody plants. Our study aimed to (1) compare extracellular PO activities (lignin peroxidase, Mn peroxidase, laccase, and tyrosinase) in differentially virulent strains (inhabiting winter rye in a single field) of the phytopathogenic species, Microdochium nivale; (2) check whether these activities are responsive to host plant metabolites; and (3) search for correlations between the activities, lignin-decomposing capacity, and virulence. All strains displayed all four enzymatic activities, but their levels and dynamics depended on the particular strain. The activities displayed the hallmarks of co-regulation and responsiveness to the host plant extract. No relationships between the virulence of strains and levels of their extracellular PO activities or lignin-degrading capacity were revealed. We consider that different strains may rely on different POs for plant colonization, and that different POs contribute to the “uniqueness” of the enzymatic cocktails that are delivered into host plant tissues by different virulent strains of M. nivale. Our study supports the hypothesis of the differential behavior of closely related M. nivale strains, and discusses an important role of POs in the interactions of phytopathogens with herbaceous plants.
Clustering Molecular Subtypes in Breast Cancer, Immunohistochemical Parameters and Risk of Axillary Nodal Involvement
Authors: Pereira Augusto , Siegrist Jaime , Lizarraga Santiago , Pérez-Medina Tirso
Published: August 2022

(1) Background: To establish similarities in the risk of axillary lymph node metastasis between different groups of women with breast cancer according to immunohistochemical (IHC) parameters. (2) Methods: Data was collected retrospectively, from 2000 to 2013, of 1058 node-positive breast tumours. All patients were divided according to the St Gallen 2013 criteria and IHC features. The proportion of axillary involvement (pN > pN0; pN > pN1mi; pN > pN1) was calculated for each group. Similarities in axillary nodal dissemination were explored by cluster analysis and association between IHC and risk of axillary disease was studied with multivariate analysis. (3) Results: Among clinico-pathological surrogates of intrinsic subtypes, axillary involvement was more frequent in Luminal-B like HER2 negative (45.8%) and less frequent in Luminal-B HER2 positive (33.8%; p = 0.044). Axillary macroscopic involvement was more frequent in Luminal-B like HER2 negative (37.9%) and HER2 positive (37.8%) and less frequent in Luminal-B HER2 positive (25.5%) and Luminal-A like (25.6%; p = 0.002). Axillary involvement ≥pN2 was significantly less frequent in Luminal-A like (7.4%; p < 0.001). Luminal-A with Luminal-B HER2 positive, and triple-negative with Erb-B2 overexpressing tumours were clustered together regarding any axillary involvement, macroscopic disease or ≥pN2. Among the defined subgroups, axillary metastases were more frequent when Ki67 was higher. In a multivariate analysis, Ki67>14% were associated with a risk of axillary metastases (HR: 1.31; 95% CI, 1.51–6.80; p < 0.037). (4) Conclusions: there are two lymphatic drainage pathways of the breast according to the expression of hormone receptor-related genes. Positive-ER tumors are associated with lower axillary involvement and negative-ER tumors and Ki67 > 14% with higher nodal involvement.
What Affects the Use Flexibility of Pocket Parks? Evidence from Nanjing, China
Authors: Zhou Conghui , Xie Meng , Zhao Jin , An Yihuan
Published: August 2022

The use flexibility of pocket parks is one of the essential factors that determine their service compatibility, efficiency, and attraction for park users in densely populated cities. To examine the factors that affect the use flexibility of pocket parks, we collected data on the periodic use of 74 pocket parks through a field survey in Nanjing and adopted the Shannon Wiener diversity index to quantitatively measure this variable. After using a multiple regression model series, we examined the internal and surrounding factors that affect the use flexibility of pocket parks on weekdays and weekends, respectively, and compared them with those that affect the use intensity of pocket parks. The results showed that paved ground and the mixed use of surrounding land promoted both use flexibility and intensity of pocket parks. Boundary buildings and surrounding residents only promoted their use flexibility, while tree canopies and exercising facilities only promoted the use intensity of pocket parks. A significant correlation (p < 0.05) was also found between the use flexibility and intensity on weekdays. These findings can contribute to future decisions regarding pocket park planning and design in Nanjing and similar cities.
Spatial Dynamics and Determinants of Population Urbanization in the Upper Reaches of the Yellow River
Authors: Zhao Lianchun , Zhao Chengzhang , Huang Jiajing
Published: August 2022

The spatiotemporal evolution of population urbanization and its relationship with economic variables are important aspects of socioeconomic research and essential for revealing the mechanism of urban construction and evolution. To study the spatial heterogeneity and influencing mechanisms of population urbanization in the upper reaches of the Yellow River, this study investigated the spatial distribution and dynamic evolution of population urbanization using nuclear density analysis, exploratory spatial data analysis and the geographical detector model. The results showed that the rate of population urbanization in the upper reaches of the Yellow River increased annually since 2000 and that the nuclear density curve changed from a single–peaked mode in 2000 to a double–peaked mode in 2018. The spatial distribution of the population urbanization level was uneven, that is, high in the north and low in the south, with substantial spatial agglomeration and spatial autocorrelation. The main distribution of hot spots was in the Yinchuan area in the north, while cold spots were distributed mainly in the south. Analysis revealed that changes in population urbanization level were mainly attributable to the influence of economic and employment opportunities, such as regional GDP, GDP per capita, proportion of tertiary industry in GDP, and total retail sales of consumer goods.
Modelling Land Use in The Gold Belt Territories of Iron Age Southern Zambezia
Authors: Nyamushosho Robert T. , Chirikure Shadreck , Sitas Ari , Maṱhoho Eric N.
Published: August 2022

Throughout the world, the entanglement of humans and landscapes varies from area to area depending on the time scale. In southern Africa, the impact of humanity on the physical environment is largely discussed in the context of modern rural and urban societies, and, usually, most contributions come from human geography, agriculture, and earth sciences. Very limited research is usually extended into the deep past, yet the archaeological record is replete with valuable information that gives a long-time depth of past human land use practices. Consequently, the contribution of the physical environment to the development of complexity over time remains poorly understood in most parts of Iron Age (CE 200–1900) southern Zambezia, particularly in Mberengwa and other gold-belt territories that have often received cursory research attention. What remains obscured is how did inhabitants of these gold-belt territories transform their landscapes in the long and short-term and how did these transformations intersect with their everyday lives? In this study, we combined archaeological, historical, and anthropological data of the Zimbabwe tradition societies that lived in ancient Mberengwa to probe these issues. The preliminary outcome suggests that despite vulnerability to high temperatures, tsetse-flies, and low rainfall, Later Iron Age societies that inhabited this gold belt territory were innovative risk-takers who successfully adapted a mix of land use practices to achieve longevity in settlement and prosperity in agropastoralism, mining, crafting, and much more. This proffers useful lessons on sustainable land use. Hopefully, with modification to suit the present, such solutions may help policy makers and modern societies living in similar environments to combat current global challenges related to environmental change.
Land Cover and Human Disturbance Impact on Water Chemistry and Ecological Health in an Asian Temperate Lotic System
Authors: Mamun Md , Kim Jeong-Eun , An Kwang-Guk
Published: August 2022

The ecological integrity of lotic ecosystems is influenced by land cover type and human activity throughout the watershed. This study evaluated Nakdong River conditions in 2016 using two multi-metric models, the index of biotic integrity (IBI) and the water pollution index (WPI), and compared model outputs for four land cover types: predominantly urban, forest, barren land, and agricultural. The primary objective of this study was to determine whether the land cover type and human disturbance metrics effectively regulate water quality, fish communities, and ecological integrity in the Nakdong River basin. Predominantly forest sites had low nutrient, organic matter, suspended solids, ion, and algal chlorophyll concentrations. In contrast, these concentrations were higher in predominantly agricultural, urban, and barren land areas. Concentrations of nutrients, organic matter, ions, suspended particle loadings, and algal growth regulated by the intensity of the Asian summer monsoon. Model outputs indicated that total phosphorus (TP) was the most important factor in algal growth in agricultural (R2 = 0.25) and barren land (R2 = 0.35) sites, and evidence of P limitation was found, with TN:TP ratios >17 in ambient water. Fish community analysis indicated that tolerant species dominated the fish community in the agricultural (52%), barren land (85%), and urban sites (53%), and sensitive species were dominant in the forest sites (56%). Fish composition analysis indicated that two exotic species (Lepomis macrochirus (3.99%) and Micropterus salmoides (3.92%)) were identified as the fifth and seventh most abundant fish species in the watershed and labeled as “ecologically disturbing species” in Korea. Nutrient enrichment, organic pollution, and algal blooms enhanced the mean relative abundance of omnivorous and tolerant fish species. Mean WPI and IBI scores indicated fair or poor conditions in the agricultural (WPI: 22, IBI: 16), barren land (WPI: 21, IBI: 14), and urban (WPI: 21, IBI: 17) sites and good or fair conditions in forest (WPI: 28, IBI: 21) sites. The chemical (r = −0.34) and biological (r = −0.21) health of the river basin were negatively related to human disturbance metrics. The findings suggested that regional land cover, summer monsoon intensity, and human disturbance are important drivers of water quality, fish community, and ecological health. The resulting information suggested that agricultural diffuse pollution control, cutting-edge wastewater treatment technologies, and reducing the degrees of human disturbance could improve the Nakdong River’s ecological integrity.
UV-Induced Somatic Mutations Driving Clonal Evolution in Healthy Skin, Nevus, and Cutaneous Melanoma
Authors: Loras Alba , Gil-Barrachina Marta , Marqués-Torrejón María Ángeles , Perez-Pastor Gemma , Martinez-Cadenas Conrado
Published: August 2022

Introduction: Due to its aggressiveness, cutaneous melanoma (CM) is responsible for most skin cancer-related deaths worldwide. The origin of CM is closely linked to the appearance of UV-induced somatic mutations in melanocytes present in normal skin or in CM precursor lesions (nevi or dysplastic nevi). In recent years, new NGS studies performed on CM tissue have increased the understanding of the genetic somatic changes underlying melanomagenesis and CM tumor progression. Methods: We reviewed the literature using all important scientific databases. All articles related to genomic mutations in CM as well as normal skin and nevi were included, in particular those related to somatic mutations produced by UV radiation. Conclusions: CM development and progression are strongly associated with exposure to UV radiation, although each melanoma subtype has different characteristic genetic alterations and evolutionary trajectories. While BRAF and NRAS mutations are common in the early stages of tumor development for most CM subtypes, changes in CDKN2A, TP53 and PTEN, together with TERT promoter mutations, are especially common in advanced stages. Additionally, large genome duplications, loss of heterozygosity, and copy number variations are hallmarks of metastatic disease. Finally, the mutations driving melanoma targeted-therapy drug resistance are also summarized. The complete sequential stages of clonal evolution leading to CM onset from normal skin or nevi are still unknown, so further studies are needed in this field to shed light on the molecular pathways involved in CM malignant transformation and in melanoma acquired drug resistance.
1,2,3-Triazole-Benzofused Molecular Conjugates as Potential Antiviral Agents against SARS-CoV-2 Virus Variants
Authors: Al-Humaidi Jehan Y. , Shaaban Marwa M. , Rezki Nadjet , Aouad Mohamed R. , Zakaria Mohamed , Jaremko Mariusz , Hagar Mohamed , Elwakil Bassma H.
Published: August 2022

SARS-CoV-2 and its variants, especially the Omicron variant, remain a great threat to human health. The need to discover potent compounds that may control the SARS-CoV-2 virus pandemic and the emerged mutants is rising. A set of 1,2,3-triazole and/or 1,2,4-triazole was synthesized either from benzimidazole or isatin precursors. Molecular docking studies and in vitro enzyme activity revealed that most of the investigated compounds demonstrated promising binding scores against the SARS-CoV-2 and Omicron spike proteins, in comparison to the reference drugs. In particular, compound 9 has the highest scoring affinity against the SARS-CoV-2 and Omicron spike proteins in vitro with its IC50 reaching 75.98 nM against the Omicron spike protein and 74.51 nM against the SARS-CoV-2 spike protein. The possible interaction between the synthesized triazoles and the viral spike proteins was by the prevention of the viral entry into the host cells, which led to a reduction in viral reproduction and infection. A cytopathic inhibition assay in the human airway epithelial cell line (Vero E6) infected with SARS-CoV-2 revealed the effectiveness and safety of the synthesized compound (compound 9) (EC50 and CC50 reached 80.4 and 1028.28 µg/mL, respectively, with a selectivity index of 12.78). Moreover, the antiinflammatory effect of the tested compound may pave the way to reduce the reported SARS-CoV-2-induced hyperinflammation.
Feasibility Study of a Novel Magnetic Bone Cement for the Treatment of Bone Metastases
Authors: Ren Bowen , Han Zhenchuan , Li Wenyi , Liu Jianheng
Published: August 2022

Bone cement is a crucial material to treat bone metastases defects, and can fill the bone defect and provide mechanical support simultaneously, but the antitumor effect is very limited. Magnetic bone cement not only supports bone metastasis defects but can also achieve magnetic hyperthermia to eliminate tumor cells around the bone defect. However, the physicochemical properties of the bone cement matrix will change if the weight ratio of the magnetic nanoparticles in the cement is too high. We mixed 1 weight percent Zn0.3Fe2.7O4 with good biocompatibility and high heating efficiency into a polymethyl methacrylate matrix to prepare magnetic bone cement, which minimized the affection for physicochemical properties and satisfied the hyperthermia requirement of the alternating magnetic field.
Real and Simulated Microgravity: Focus on Mammalian Extracellular Matrix
Authors: Andreeva Elena , Matveeva Diana , Zhidkova Olga , Zhivodernikov Ivan , Kotov Oleg , Buravkova Ludmila
Published: August 2022

The lack of gravitational loading is a pivotal risk factor during space flights. Biomedical studies indicate that because of the prolonged effect of microgravity, humans experience bone mass loss, muscle atrophy, cardiovascular insufficiency, and sensory motor coordination disorders. These findings demonstrate the essential role of gravity in human health quality. The physiological and pathophysiological mechanisms of an acute response to microgravity at various levels (molecular, cellular, tissue, and physiological) and subsequent adaptation are intensively studied. Under the permanent gravity of the Earth, multicellular organisms have developed a multi-component tissue mechanosensitive system which includes cellular (nucleo- and cytoskeleton) and extracellular (extracellular matrix, ECM) “mechanosensory” elements. These compartments are coordinated due to specialized integrin-based protein complexes, forming a distinctive mechanosensitive unit. Under the lack of continuous gravitational loading, this unit becomes a substrate for adaptation processes, acting as a gravisensitive unit. Since the space flight conditions limit large-scale research in space, simulation models on Earth are of particular importance for elucidating the mechanisms that provide a response to microgravity. This review describes current state of art concerning mammalian ECM as a gravisensitive unit component under real and simulated microgravity and discusses the directions of further research in this field.
Magnetic Anchoring Considerations for Retractors Supporting Manual and Robot-Assisted Minimally Invasive Surgery
Authors: Nigicser Illés , Oldfield Matthew , Haidegger Tamás
Published: August 2022

The rise and advancement of minimally invasive surgery (MIS) has significantly improved patient outcomes, yet its technical challenges—such as tissue manipulation and tissue retraction—are not yet overcome. Robotic surgery offers some compensation for the ergonomic challenges, as retraction typically requires an extra robotic arm, which makes the complete system more costly. Our research aimed to explore the potential of rapidly deployable structures for soft tissue actuation and retraction, developing clinical and technical requirements and putting forward a critically evaluated concept design. With systematic measurements, we aimed to assess the load capacities and force tolerance of different magnetic constructions. Experimental and simulation work was conducted on the magnetic coupling technology to investigate the conditions where the clinically required lifting force of 11.25 N could be achieved for liver retraction. Various structure designs were investigated and tested with N52 neodymium magnets to create stable mechanisms for tissue retraction. The simplified design of a new MIS laparoscopic instrument was developed, including a deployable structure connecting the three internal rod magnets with joints and linkages that could act as an actuator for liver retraction. The deployable structure was designed to anchor strings or bands that could facilitate the lifting or sideways folding of the liver creating sufficient workspace for the target upper abdominal procedures. The critical analysis of the project concluded a notable potential of the developed solution for achieving improved liver retraction with minimal tissue damage and minimal distraction of the surgeon from the main focus of the operation, which could be beneficial, in principle, even at robot-assisted procedures.
On the Robustness and Sensitivity of Several Nonparametric Estimators via the Influence Curve Measure: A Brief Study
Authors: Ghosh Indranil , Fleming Kathleen
Published: August 2022

The use of influence curve as a measure of sensitivity is not new in the literature but has not been properly explored to the best of our knowledge. In particular, the mathematical derivation of the influence function for several popular nonparametric estimators (such as trimmed mean, α-winsorized mean, Pearson product moment correlation coefficient etc. among notable ones) is not given in adequate detail. Moreover, the summary of the final expressions given in some sporadic cases does not appear to be correct. In this article, we aim to examine and summarize the derivation of the influence curve for various well-known estimators for estimating the location of a population, many of which are considered in the nonparametric paradigm.
Graphs of Stable Gauss Maps and Quine’s Theorem for Oriented 2-Manifolds
Authors: Mendes de Jesus Catarina , Romero Pantaleón D.
Published: August 2022

In this paper we will study the topology of the singular set of stable Gauss maps from closed orientable surfaces immersed in the 3-space from a global point of view and we will obtain a relationship between the Euler Characteristic of the regular components and the signs of the cusps.
Fixed-Point Results for Meir–Keeler Type Contractions in Partial Metric Spaces: A Survey
Authors: Karapınar Erdal , Agarwal Ravi P. , Yeşilkaya Seher Sultan , Wang Chao
Published: August 2022

In this paper, we aim to review Meir–Keeler contraction mappings results on various abstract spaces, in particular, on partial metric spaces, dislocated (metric-like) spaces, and M-metric spaces. We collect all significant results in this direction by involving interesting examples. One of the main reasons for this work is to help young researchers by giving a framework for Meir Keeler’s contraction.
The State of Research in Fracture-Related Infection—A Bibliometric Analysis
Authors: Walter Nike , Orbenes Nicolás , Rupp Markus , Alt Volker
Published: August 2022

Background and Objectives: Fracture-related infection (FRI) is a challenging complication in trauma surgery. A consensus definition of FRI has only recently been published. Therefore, the purpose of this study was to evaluate the state of research related to FRI. Material and Methods: A systemic literature review was conducted on research on FRI published between 2017 and 2020. The Web of Science database was used, and a bibliometric analysis was performed. To provide robust evidence regarding the impact of publications, the behavior of publications in non-traditional dissemination channels was analyzed. For this, the Research Interest Score and the Altmetric Score were combined. The Research Interest Score was calculated from information extracted from ResearchGate, while Altmetric Score includes information from different websites and apps with a significant volume of traffic, such as Twitter. Results: A total of 131 published papers were identified. The most significant contribution came from the United States and European countries. The most relevant articles were published by the journal Injury—International Journal of the Care of the Injured. A positive correlation was observed between the number of citations and Research Interest Scores, whereas the number of citations and Altmetric Score showed no correlation. The social media platform most used by FRI researchers was Twitter. Conclusions: By evaluating the status of publications for FRI between 2017 and 2020, an upward trend in the number of publications was evident. This could be related to the increasing acceptance of the long-needed definition for FRI and the implications it carries for daily clinical practice.
Single Level Spondylolisthesis Associated Sagittal Plane Imbalance Corrected by Pre-Psoas Interbody Fusion Using Anterior Column Release with 30° Expandable Hyperlordotic Cage
Authors: Mathkour Mansour , Shapiro Stephen Z. , Scullen Tyler , Werner Cassidy , Kilgore Mitchell D. , Chavarro Velina S. , Denis Daniel R.
Published: August 2022

Background: Loss of lumbar lordosis caused by single level degenerative spondylolisthesis can trigger significant sagittal plane imbalance and failure to correct lumbopelvic parameters during lumbar fusion can lead to poor outcome or worsening deformity. Anterior column release (ACR) through a pre-psoas approach allows the placement of a hyperlordotic cage (HLC) to improve lumbar lordosis, but it is unclear if the amount of cage lordosis affects radiological outcomes in real-life patient conditions. Methods: Three patients were treated with ACR and 30° expandable HLC for positive sagittal imbalance secondary to single-level spondylolisthesis. Patients reported baseline and post-operative Oswestry Disability Index (ODI) and Numeric Pain Score (NRS). Radiographic parameters of sagittal balance included lumbar lordosis (LL), sagittal vertical axis (SVA) and pelvic incidence-lumbar lordosis mismatch (PI-LL). Results: Surgical indications were sagittal plane imbalance caused by L4–L5 degenerative spondylolisthesis (n = 2) and L3–L4 spondylolisthesis secondary to adjacent segmental degeneration (n = 1). Average post-operative length of stay was 3 days (range 2–4) and estimated blood loss was 266 mL (range 200–300). NRS and ODI improved in all patients. All experienced improvements in LL (x¯preop = 33°, x¯postop = 56°), SVA (x¯preop = 180 mm, x¯postop = 61 mm) and PI-LL (x¯preop = 26°, x¯postop = 5°). Conclusion: ACR with expandable HLC can restore sagittal plane balance associated with single-level spondylolisthesis. Failure to perform ACR with HLC placement during pre-psoas interbody fusion may result in under correction of lordosis and poorer outcome for these patients.
Implementation of the Helsinki Model at West Tallinn Central Hospital
Authors: Gross-Paju Katrin , Thomson Ulvi , Adlas Raul , Jaakmees Helle , Kannel Karin , Mallene Sandra Marii , Mironenko Svetlana , Reitsnik Agnes , Vares Ain , Ütt Sandra
Published: August 2022

Ischemic stroke is defined as neurological deficit caused by brain infarction. The intravenous tissue plasminogen activator, alteplase, is an effective treatment. However, efficacy of this method is time dependent. An important step in improving outcome and increasing the number of patients receiving alteplase is the shortening of waiting times at the hospital, the so-called door-to-needle time (DNT). The comprehensive Helsinki model was proposed in 2012, which enabled the shortening of the DNT to less than 20 min. Background and Objectives: The aim of this study was to analyze the transferability of the suggested model to the West Tallinn Central Hospital (WTCH). Materials and Methods: Since the first thrombolysis in 2005, all patients are registered in the WTCH thrombolysis registry. Several steps following the Helsinki model have been implemented over the years. Results: The results demonstrate that the number and also the percent of thrombolysed stroke patients increased during the years, from a few thrombolysis annually, to 260 in 2021. The mean DNT dropped significantly to 33 min after the implementation of several steps, from the emergency medical services (EMS) prenotification with a phone call to the neurologists, to the setting-up of a thrombolysis team based in the stroke unit. Also, the immediate start of treatment using a computed tomography table was introduced. Conclusions: In conclusion, several implemented steps enabled the shortening of the DNT from 30 to 25.2 min. Short DNTs were achieved and maintained only with EMS prenotification.
Salt, Not Always a Cardiovascular Enemy? A Mini-Review and Modern Perspective
Authors: Hogas Mihai , Statescu Cristian , Padurariu Manuela , Ciobica Alin , Bilha Stefana Catalina , Haisan Anca , Timofte Daniel , Hogas Simona
Published: August 2022

Dietary salt intake is a long-debated issue. Increased sodium intake is associated with high blood pressure, leading to salt-sensitive hypertension. Excessive salt intake leads to arterial stiffness in susceptible individuals via impaired nitric oxide action and increased endothelin-1 expression, overactivity of the renal sympathetic nervous system and also via aldosterone-independent activation of the mineralocorticoid receptor. Salt restriction in such individuals reduces blood pressure (BP) values. The optimal level of salt restriction that leads to improved cardiovascular outcomes is still under debate. Current BP and dietary guidelines recommend low sodium intake for the general population. However, a specific category of patients does not develop arterial hypertension in response to sodium loading. In addition, recent research demonstrates the deleterious effects of aggressive sodium restriction, even in heart failure patients. This mini review discusses current literature data regarding the advantages and disadvantages of salt restriction and how it impacts the overall health status.
Direct Current Annealing Modulated Ordered Structure to Optimize Tensile Mechanical Properties of Co-Based Amorphous Metallic Microwires
Authors: Wang Congliang , Cao Guanyu , Liu Jingshun , Zhang Yun , Liu Rui , Wang Feng , Zhang Mingwei , Wang Lu , Zhang Bo
Published: August 2022

Herein, the ordered structure of Co-based metallic microwires was modulated by direct current-annealing, thereby improving the tensile mechanical properties. Based on the thermophysical parameters of the metallic microwires, the annealing current intensities of 65 mA, 90 mA and 150 mA were determined by the method of numerical calculation. The experimental results indicated that the ordered structure of the metallic microwires was regulated under the action of Joule heating, and with the rising of the annealing current, the ordered structure increased and the distribution tended to be concentrated. The 90 mA current-annealed metallic microwires have favorable tensile mechanical properties and fracture reliability, with the tensile strength and elongation of 4540.10 MPa and 2.99%, respectively, and the fracture threshold is 1910.90 MPa. Both the as-cast and current-annealed metallic microwires were brittle fractures, and the fractures consisted of shear deformation regions and crack extension regions. The improvement of the mechanical properties of metallic microwires is related to the nano-ordered structure and their distribution. Under the condition of 90 mA current annealing, the uniformly distributed nano-ordered structures were formed in the amorphous matrix of the metallic microwires, which can effectively slow down the expansion of the shear bands and reduce the possibility of crack generation. This study provides process reference and theoretical guidance for the application of Co-based metallic microwires in the field of stress sensors.