bluuebunny/crossref_metadata_2025 huggingface dataset:
Endothelial Dysfunction after Hematopoietic Stem Cell Transplantation: A Review Based on Physiopathology
Authors: Milone Giuseppe , Bellofiore Claudia , Leotta Salvatore , Milone Giulio Antonio , Cupri Alessandra , Duminuco Andrea , Garibaldi Bruno , Palumbo Giuseppe
Published: January 2022

Endothelial dysfunction (ED) is frequently encountered in transplant medicine. ED is an argument of high complexity, and its understanding requires a wide spectrum of knowledge based on many fields of basic sciences such as molecular biology, immunology, and pathology. After hematopoietic stem cell transplantation (HSCT), ED participates in the pathogenesis of various complications such as sinusoidal obstruction syndrome/veno-occlusive disease (SOS/VOD), graft-versus-host disease (GVHD), transplant-associated thrombotic microangiopathy (TA-TMA), idiopathic pneumonia syndrome (IPS), capillary leak syndrome (CLS), and engraftment syndrome (ES). In the first part of the present manuscript, we briefly review some biological aspects of factors involved in ED: adhesion molecules, cytokines, Toll-like receptors, complement, angiopoietin-1, angiopoietin-2, thrombomodulin, high-mobility group B-1 protein, nitric oxide, glycocalyx, coagulation cascade. In the second part, we review the abnormalities of these factors found in the ED complications associated with HSCT. In the third part, a review of agents used in the treatment of ED after HSCT is presented.
Serum Levels of Acylcarnitines and Amino Acids Are Associated with Liberation from Organ Support in Patients with Septic Shock
Authors: Jennaro Theodore S. , Viglianti Elizabeth M. , Ingraham Nicholas E. , Jones Alan E. , Stringer Kathleen A. , Puskarich Michael A.
Published: January 2022

Sepsis-induced metabolic dysfunction is associated with mortality, but the signatures that differentiate variable clinical outcomes among survivors are unknown. Our aim was to determine the relationship between host metabolism and chronic critical illness (CCI) in patients with septic shock. We analyzed metabolomics data from mechanically ventilated patients with vasopressor-dependent septic shock from the placebo arm of a recently completed clinical trial. Baseline serum metabolites were measured by liquid chromatography-mass spectrometry and 1H-nuclear magnetic resonance. We conducted a time-to-event analysis censored at 28 days. Specifically, we determined the relationship between metabolites and time to extubation and freedom from vasopressors using a competing risk survival model, with death as a competing risk. We also compared metabolite concentrations between CCI patients, defined as intensive care unit level of care ≥ 14 days, and those with rapid recovery. Elevations in two acylcarnitines and four amino acids were related to the freedom from organ support (subdistributional hazard ratio < 1 and false discovery rate < 0.05). Proline, glycine, glutamine, and methionine were also elevated in patients who developed CCI. Our work highlights the need for further testing of metabolomics to identify patients at risk of CCI and to elucidate potential mechanisms that contribute to its etiology.
Cardiovascular Function and Exercise Capacity in Childhood Cancer Survivors
Authors: Reiner Barbara , Schmid Irene , Schulz Thorsten , Müller Jan , Hager Alfred , Hock Julia , Ewert Peter , Wolf Cordula , Oberhoffer-Fritz Renate , Weil Jochen
Published: January 2022

Introduction: Childhood cancer survivors (CCS) might be at high risk of additional chronic diseases due to cardiotoxic side effects. The aim of this study was to analyze long-term side effects of cancer therapy on vascular structure/function, cardiac biomarkers and on physical activity. Methods: In total, 68 asymptomatic patients aged 16–30 years with childhood cancer (diagnosed 10.6 ± 3.9 years ago) were examined from 2015–2020. (Central) blood pressure and pulse wave velocity were registered via the oscillometric method, while carotid intima-media thickness (cIMT) was measured non-invasively by ultrasound. cIMT values of patients were compared to healthy controls (n = 68; aged 22.3 ± 3.5 years). Patients’ exercise capacity was recorded. The plasma N-terminal pro-brain natriuretic protein (NTproBNP) and troponin levels were measured as cardiac biomarkers. CCS were categorized in groups with low, moderate and high anthracyclines. Results: No differences were found in cIMT between patients and controls as well as between patients with various anthracycline dosage. Patients with high dose anthracyclines showed a significant lower performance versus patients with moderate dose anthracyclines (84.4% of predicted VO2peak; p = 0.017). A total of 11.6% of CCS had abnormal NTproBNP values which correlated with received anthracycline dosage (p = 0.024; r = 0.343). Conclusion: NTproBNP levels and exercise capacity might be early markers for cardiovascular dysfunction in CCS and should be included in a follow-up protocol, while cIMT and troponin seem not to be adequate parameters.
Radiological Evaluation of Combined Anteroposterior Fusion with Vertebral Body Replacement Using a Minimally Invasive Lateral Approach for Osteoporotic Vertebral Fractures: Verification of Optimal Surgical Procedure
Authors: Takeuchi Takumi , Yamagishi Kenichiro , Konishi Kazumasa , Sano Hideto , Takahashi Masato , Ichimura Shoichi , Kono Hitoshi , Hasegawa Masaichi , Hosogane Naobumi
Published: January 2022

The combined anteroposterior fusion with vertebral body replacement (VBR) using a wide footplate expandable cage with a minimally invasive lateral approach has been widely used for pseudoarthrosis after osteoporotic vertebral fractures. The purpose of this study is to evaluate the radiological results of combined anteroposterior surgery using VBR and to recommend the optimal procedure. Thirty-eight elderly patients were included in this study. The mean preoperative local kyphosis angle was 29.3°, and the mean correction loss angle was 6.3°. Cage subsidence was observed in ten patients (26.3%), and UIV or LIV fracture in twelve patients (31.6%). Patients with cage subsidence were compared to those without cage subsidence to determine the causal factors. The mean number of fixed vertebrae was 5.4 vertebrae with cage subsidence and 7.4 vertebrae without cage subsidence. In addition, to precisely clarify the optimal number of fixed vertebrae, those patients with two above–two below fixation were compared to those with less than two above–two below fixation, which revealed that the correction loss angle was significantly less in two above–two below fixation (p = 0.016). Based on these results, we recommend at least two above–two below fixation with VBR to minimize the correction loss angle and prevent cage subsidence.
Preparation of Gellan Gum-Inorganic Composite Film and Its Metal Ion Accumulation Property
Authors: Yamada Masanori , Kametani Yoshihiro
Published: January 2022

Gellan gum is one of the water-soluble anionic polysaccharides produced by the bacteria Sphingomonas elodea. In this study, we prepared gellan gum-inorganic composite films by mixing the gellan gum and a silane coupling reagent—3-glycidoxypropyltrimethoxysilane (GPTMS). These gellan gum-GPTMS composite films were stable in an aqueous solution and showed a thermal stability. In addition, these composite films indicated a mechanical strength by the formation of the three-dimensional network of siloxane. We demonstrated the accumulation of metal ions from a metal ion-containing aqueous solution by the composite film. As a result, although the composite film indicated the accumulation of heavy and rare-earth metal ions, the light metal ions, such as Mg(II) and Al(III) ions, did not interact with the composite material. Therefore, the accumulative mechanism of metal ions using a composite film was evaluated by IR measurements. As a consequence, although the accumulation of heavy and rare-earth metal ions occurred at both the −COO− group and the −OH group in the gellan gum, the accumulation of light metal ions occurred only at the −OH group.
A Novel Approach for Real-Time Quality Monitoring in Machining of Aerospace Alloy through Acoustic Emission Signal Transformation for DNN
Authors: Adeniji David , Oligee Kyle , Schoop Julius
Published: January 2022

Gamma titanium aluminide (γ-TiAl) is considered a high-performance, low-density replacement for nickel-based superalloys in the aerospace industry due to its high specific strength, which is retained at temperatures above 800 °C. However, low damage tolerance, i.e., brittle material behavior with a propensity to rapid crack propagation, has limited the application of γ-TiAl. Any cracks introduced during manufacturing would dramatically lower the useful (fatigue) life of γ-TiAl components, making the workpiece surface’s quality from finish machining a critical component to product quality and performance. To address this issue and enable more widespread use of γ-TiAl, this research aims to develop a real-time non-destructive evaluation (NDE) quality monitoring technique based on acoustic emission (AE) signals, wavelet transform, and deep neural networks (DNN). Previous efforts have opted for traditional approaches to AE signal analysis, using statistical feature extraction and classification, which face challenges such as the extraction of good/relevant features and low classification accuracy. Hence, this work proposes a novel AI-enabled method that uses a convolutional neural network (CNN) to extract rich and relevant features from a two-dimensional image representation of 1D time-domain AE signals (known as scalograms), subsequently classifying the AE signature based on pedigreed experimental data and finally predicting the process-induced surface quality. The results of the present work show good classification accuracy of 80.83% using scalogram images, in-situ experimental data, and a VGG-19 pre-trained neural network, establishing the significant potential for real-time quality monitoring in manufacturing processes.
Nurses’ Practices in the Peripheral Intravenous Catheterization of Adult Oncology Patients: A Mix-Method Study
Authors: Santos-Costa Paulo , Paiva-Santos Filipe , Sousa Liliana B. , Bernardes Rafael A. , Ventura Filipa , Fearnley William David , Salgueiro-Oliveira Anabela , Parreira Pedro , Vieira Margarida , Graveto João
Published: January 2022

A significant number of adult oncology patients require at least one peripheral intravenous catheter to fulfill their therapeutic plan. Recent evidence indicates that catheter failure rates are high in this cohort, impacting care outcomes and patient experience during cancer treatment. This reality represents a challenge to nurses worldwide since in most international settings they are responsible for delivering quality care during the insertion and maintenance of such devices. This study aims to explore current nursing practices regarding the insertion, maintenance, and surveillance of peripheral intravenous catheters in oncology patients. A two-phase mix-method study was conducted with the nursing team from the surgical ward of a large oncology hospital in Portugal. In phase one (observational prospective study), nurses’ practices during catheter insertion and maintenance were observed by the research team and recorded using standardized instruments and validated scales. In phase two, three online focus groups were conducted with the nursing team to present the results observed in phase one and explore their perceptions of current practices. All ethical principles were assured throughout the study. Significant divergent practices were observed and identified by the nurses, especially concerning patient involvement, nurses’ adherence to the aseptic, non-touch technique, catheter stabilization and dressing, and catheter flushing and locking. Such practices may partially explain the high complication rate found (26%) and substantiate the need for future intervention in this field.
Machine Learning Prediction of Visual Outcome after Surgical Decompression of Sellar Region Tumors
Authors: Qiao Nidan , Ma Yichen , Chen Xiaochen , Ye Zhao , Ye Hongying , Zhang Zhaoyun , Wang Yongfei , Lu Zhaozeng , Wang Zhiliang , Xiao Yiqin , Zhao Yao
Published: January 2022

Introduction: This study aims to develop a machine learning-based model integrating clinical and ophthalmic features to predict visual outcomes after transsphenoidal resection of sellar region tumors. Methods: Adult patients with optic chiasm compression by a sellar region tumor were examined to develop a model, and an independent retrospective cohort and a prospective cohort were used to validate our model. Predictors included demographic information, and ophthalmic and laboratory test results. We defined “recovery” as more than 5% for a p-value in mean deviation compared with the general population in the follow-up. Seven machine learning classifiers were employed, and the best-performing algorithm was selected. A decision curve analysis was used to assess the clinical usefulness of our model by estimating net benefit. We developed a nomogram based on essential features ranked by the SHAP score. Results: We included 159 patients (57.2% male), and the mean age was 42.3 years old. Among them, 96 patients were craniopharyngiomas and 63 patients were pituitary adenomas. Larger tumors (3.3 cm vs. 2.8 cm in tumor height) and craniopharyngiomas (73.6%) were associated with a worse prognosis (p < 0.001). Eyes with better outcomes were those with better visual field and thicker ganglion cell layer before operation. The ensemble model yielded the highest AUC of 0.911 [95% CI, 0.885–0.938], and the corresponding accuracy was 84.3%, with 0.863 in sensitivity and 0.820 in specificity. The model yielded AUCs of 0.861 and 0.843 in the two validation cohorts. Our model provided greater net benefit than the competing extremes of intervening in all or no patients in the decision curve analysis. A model explanation using SHAP score demonstrated that visual field, ganglion cell layer, tumor height, total thyroxine, and diagnosis were the most important features in predicting visual outcome. Conclusion: SHAP score can be a valuable resource for healthcare professionals in identifying patients with a higher risk of persistent visual deficit. The large-scale and prospective application of the proposed model would strengthen its clinical utility and universal applicability in practice.
Detyrosinated α-Tubulin, Vimentin and PD-L1 in Circulating Tumor Cells (CTCs) Isolated from Non-Small Cell Lung Cancer (NSCLC) Patients
Authors: Katsarou Spyridoula D. , Messaritakis Ippokratis , Voumvouraki Anastasia , Kakavogiannis Stavros , Κotsakis Athanasios , Alkahtani Saad , Stournaras Christos , Martin Stuart S. , Georgoulias Vassilis , Kallergi Galatea
Published: January 2022

Upregulation of Vimentin (VIM), alpha-Tubulin (TUB) and Detyrosinated tubulin (GLU) in circulating tumor cells (CTCs) derived from breast cancer patients is related to poor prognosis. In the current study we evaluated for the first time, these cytoskeletal proteins in sixty Non-Small Cell Lung Cancer (NSCLC) patients’ CTCs (33 treatment-naïve and 27 pre-treated). Samples were isolated using the ISET platform and stained with a pancytokeratin (CK)/CD45/TUB, CK/GLU/VIM and CK/programmed death ligand 1 (PD-L1) combination of antibodies. Subsequently, slides were analyzed using confocal laser scanning microscopy. CTCs were detected in 86.7% of the patients. CTCs with TUB expression were identified in 65.4% (34/52) of the CK (+)-patients. GLU, VIM and PD-L1 were also evaluated. The frequency of the observed phenotypes was as follow: (CK+/GLU−/VIM−): 35.2%, (CK+/GLU+/VIM+): 63.0%, (CK+/GLU+/VIM−): 16.7%, (CK+/GLU−/VIM+): 72.2%, (CK+/PD-L1−): 75% and (CK+/PD-L1+): 55%. The OS was significantly decreased in patients with high GLU (3.8 vs. 7.9 months; p = 0.018) and/or high VIM (3.2 vs. 7.1 months; p = 0.029) expression in their CTCs. PD-L1 was also related to OS (3.4 vs. 7.21 months; p = 0.035). Moreover, TUB-high and TUB-low expression in CTCs inversely influenced patients’ OS as independent prognostic factors (p = 0.041 and p = 0.009). The current study revealed that TUB, GLU, VIM and PD-L1 were overexpressed in CTCs from NSCLC patients. Furthermore, the presence of GLU, VIM-positive and PD-L1 in CTCs is potentially related to patients’ outcomes.
Thyroid Diseases and Breast Cancer
Authors: Baldini Enke , Lauro Augusto , Tripodi Domenico , Pironi Daniele , Amabile Maria Ida , Ferent Iulia Catalina , Lori Eleonora , Gagliardi Federica , Bellini Maria Irene , Forte Flavio , Pacini Patrizia , Cantisani Vito , D’Andrea Vito , Sorrenti Salvatore , Ulisse Salvatore
Published: January 2022

Epidemiological studies aimed at defining the association of thyroid diseases with extra-thyroidal malignancies (EM) have aroused considerable interest in the possibility of revealing common genetic and environmental factors underlying disease etiology and progression. Over the years, multiple lines of evidence indicated a significant relationship between thyroid carcinomas and other primary EM, especially breast cancer. For the latter, a prominent association was also found with benign thyroid diseases. In particular, a meta-analysis revealed an increased risk of breast cancer in patients with autoimmune thyroiditis, and our recent work demonstrated that the odds ratio (OR) for breast cancer was raised in both thyroid autoantibody-positive and -negative patients. However, the OR was significantly lower for thyroid autoantibody-positive patients compared to the negative ones. This is in agreement with findings showing that the development of thyroid autoimmunity in cancer patients receiving immunotherapy is associated with better outcome and supports clinical evidence that breast cancer patients with thyroid autoimmunity have longer disease-free interval and overall survival. These results seem to suggest that factors other than oncologic treatments may play a role in the initiation and progression of a second primary malignancy. The molecular links between thyroid autoimmunity and breast cancer remain, however, unidentified, and different hypotheses have been proposed. Here, we will review the epidemiological, clinical, and experimental data relating thyroid diseases and breast cancer, as well as the possible hormonal and molecular mechanisms underlying such associations.
Assessment of a Manual Method versus an Automated, Probability-Based Algorithm to Identify Patients at High Risk for Pharmacogenomic Adverse Drug Outcomes in a University-Based Health Insurance Program
Authors: Grande Kendra J. , Dalton Rachel , Moyer Nicolas A. , Arwood Meghan J. , Nguyen Khoa A. , Sumfest Jill , Ashcraft Kristine C. , Cooper-DeHoff Rhonda M.
Published: January 2022

We compared patient cohorts selected for pharmacogenomic testing using a manual method or automated algorithm in a university-based health insurance network. The medication list was compiled from claims data during 4th quarter 2018. The manual method selected patients by number of medications by the health system’s list of medications for pharmacogenomic testing. The automated method used YouScript’s pharmacogenetic interaction probability (PIP) algorithm to select patients based on the probability that testing would result in detection of one or more clinically significant pharmacogenetic interactions. A total of 6916 patients were included. Patient cohorts selected by each method differed substantially, including size (manual n = 218, automated n = 286) and overlap (n = 41). The automated method was over twice as likely to identify patients where testing may reveal a clinically significant pharmacogenetic interaction than the manual method (62% vs. 29%, p < 0.0001). The manual method captured more patients with significant drug–drug or multi-drug interactions (80.3% vs. 40.2%, respectively, p < 0.0001), higher average number of significant drug interactions per patient (3.3 vs. 1.1, p < 0.0001), and higher average number of unique medications per patient (9.8 vs. 7.4, p < 0.0001). It is possible to identify a cohort of patients who would likely benefit from pharmacogenomic testing using manual or automated methods.
Clinical Response and Hospital Costs of Therapeutic Drug Monitoring for Vancomycin in Elderly Patients
Authors: Kim Yun , Kim Soohyun , Park Jinsook , Lee Howard
Published: January 2022

Cost-effectiveness analysis has been widely used to assess and compare the costs and benefits of a clinical service. The cost-effectiveness of vancomycin therapeutic drug monitoring (TDM) has not been studied in the elderly, who are susceptible to vancomycin-induced adverse effects. This study was performed to evaluate if vancomycin TDM is cost-effective in elderly patients in the Republic of Korea. Using the electronic medical records at a tertiary university hospital, we performed a retrospective observational study to evaluate the cost-effectiveness of vancomycin TDM in 850 elderly patients who underwent vancomycin TDM with an appropriate, recommended dosing regimen and 1094 elderly patients who did not. Cost-effectiveness variables such as clinical outcomes and medical expenses were evaluated using univariate and multivariate analyses. The TDM group spent significantly less than the non-TDM group per patient for total medical expenses (by USD 841.40) and medication expenses (by USD 16.70). However, no significant difference was noted between the TDM and non-TDM groups in clinical outcomes such as microbiological cure, prevention of nephrotoxicity, or reduced mortality, irrespective of admission to the intensive care unit. Vancomycin TDM in elderly patients was associated with economic benefits, but not with better clinical outcomes.
Root Distribution and Soil Properties of Gully Heads and Their Effects on Headcut Migration in the Mollisols Region of Northeast China
Authors: Zhang Xingyi , Qi Jiarui , Xu Jinzhong , Zhou Pengchong , Chen Zhuoxin , Wang Lixin , Guo Mingming
Published: January 2022

Previous studies have proved that root distribution along gully headwalls greatly alters soil properties and further affects the soil erodibility of gully heads. However, it is not clear whether the gully headcut migration is affected by root distribution and soil properties. Five representative gullies developed in different land uses were selected to clarify the variations of root distribution and soil properties and their effects on headcut migration in the rainy season (May to October 2021) in the Mollisols region of northeast China. Results showed that the 68.4%–93.3% of root mass density and 65.6–88.5% of root length density were concentrated in 0–30 cm soil layer of gully heads, and the roots of <2.0 mm accounted for >85%. The gullies developed in farmlands had relatively higher soil compactness, shear strength and aggregate stability, but lower organic matter (OMC), disintegration capacity and soil permeability than those developed in woodlands, unpaved roads in farmland and stable gully-beds. Changes in soil properties of gully heads were closely related to root density. The linear, areal, and volumetric migration rate of gully heads varied greatly and were 1.07–35.11 m yr−1, 28.95–562.46 m2 yr−1 and 56.82–6626.37 m3 yr−1, respectively, with the average of 9.07 m yr−1, 156.92 m2 yr−1 and 1503.02 m3 yr−1, respectively. The change in headcut migration rate was significantly affected by root density, soil properties and drainage area, of which soil texture, OMC, soil aggregate structure, and the drainage area were the critical factors influencing headcut migration in the Mollisols region of northeast China.
Tourism Business in Spanish National Parks: A Multidimensional Perspective of Sustainable Tourism
Authors: Pérez-Calderón Esteban , Miguel-Barrado Vanessa , Sánchez-Cubo Francisco
Published: January 2022

In recent years, nature tourism has increased its prominence in the tourism market due to sociocultural change and greater concern for the environment in our society. In this sense, Spanish national parks have become important tourist destinations, increasing the number of visitors significantly in the last decade, exceeding 14.81 million in 2019. In addition to their incalculable ecological value, these protected natural spaces are a key factor in achieving the socioeconomic development of their rural area of influence. The main objective of the study is to contrast the development experienced by tourist businesses in the areas of socioeconomic influence of the Spanish national parks. This has been done from a multidimensional perspective: infrastructures, socioeconomic development, and the perception of the residents belonging to the area of influence of the parks. The indicators associated with each dimension have been compiled and a logit model was used to contrast the relationships between the different variables. The results confirmed that perceived economic development and infrastructure have a significant impact on tourism businesses. In conclusion, local socioeconomic development requires greater effective public–private partnerships to achieve business prosperity and a better quality of life as factors for the sustainability of nature tourism in national parks.
Rethinking Legal Criteria for Assessing Compensation for Rural Land Expropriation: Towards a European Institutional Framework
Authors: Hernández-Alemán Anastasia , Cruz-Pérez Noelia , Santamarta Juan C.
Published: January 2022

In public management, it is common to face conflicting objectives, particularly in relation to land use. Adequate land use management requires a valuation of land that incorporates the value of all its characteristics. That is, in addition to the traditional direct use value, it must incorporate the non-use value (existence and legacy), as well as the indirect use and option values. The analytic hierarchy process is used, firstly, to identify the priority values based on a panel of experts, and secondly, in assessment of use/non-use values, using market valuation techniques as support. As a result, we analyse the trade-offs among all values, and the respondent’s consistency. At first, we observed that the soil with the highest protection had the lowest market value in terms of direct use. However, considering the weights of the panel of experts, we can conclude that the market value only represents 7.6% of the total value. Non-market values represent 92.4% of the total value. The underlying aim is to facilitate decision-making in the field of land management to increase social welfare and the resilience of landscapes.
Cell Cycle Stage and DNA Repair Pathway Influence CRISPR/Cas9 Gene Editing Efficiency in Porcine Embryos
Authors: Gutierrez Karina , Glanzner Werner G. , de Macedo Mariana P. , Rissi Vitor B. , Dicks Naomi , Bohrer Rodrigo C. , Baldassarre Hernan , Agellon Luis B. , Bordignon Vilceu
Published: January 2022

CRISPR/Cas9 technology is a powerful tool used for genome manipulation in different cell types and species. However, as with all new technologies, it still requires improvements. Different factors can affect CRISPR/Cas efficiency in zygotes, which influence the total cost and complexity for creating large-animal models for research. This study evaluated the importance of zygote cell cycle stage between early-injection (within 6 h post activation/fertilization) versus late-injection (14–16 h post activation/fertilization) when the CRISPR/Cas9 components were injected and the inhibition of the homologous recombination (HR) pathway of DNA repair on gene editing, embryo survival and development on embryos produced by fertilization, sperm injection, somatic cell nuclear transfer, and parthenogenetic activation technologies. Injections at the late cell cycle stage decreased embryo survival (measured as the proportion of unlysed embryos) and blastocyst formation (68.2%; 19.3%) compared to early-stage injection (86.3%; 28.8%). However, gene editing was higher in blastocysts from late-(73.8%) vs. early-(63.8%) injected zygotes. Inhibition of the HR repair pathway increased gene editing efficiency by 15.6% in blastocysts from early-injected zygotes without compromising embryo development. Our finding shows that injection at the early cell cycle stage along with HR inhibition improves both zygote viability and gene editing rate in pig blastocysts.
Prokineticin-Receptor Network: Mechanisms of Regulation
Authors: Lattanzi Roberta , Miele Rossella
Published: January 2022

Prokineticins are a new class of chemokine-like peptides that bind their G protein-coupled receptors, PKR1 and PKR2, and promote chemotaxis and the production of pro-inflammatory cytokines following tissue injury or infection. This review summarizes the major cellular and biochemical mechanisms of prokineticins pathway regulation that, like other chemokines, include: genetic polymorphisms; mRNA splice modulation; expression regulation at transcriptional and post-transcriptional levels; prokineticins interactions with cell-surface glycosaminoglycans; PKRs degradation, localization, post-translational modifications and oligomerization; alternative signaling responses; binding to pharmacological inhibitors. Understanding these mechanisms, which together exert substantial biochemical control and greatly enhance the complexity of the prokineticin-receptor network, leads to novel opportunities for therapeutic intervention. In this way, besides targeting prokineticins or their receptors directly, it could be possible to indirectly influence their activity by modulating their expression and localization or blocking the downstream signaling pathways.
Using Non-Pharmaceutical Interventions and High Isolation of Asymptomatic Carriers to Contain the Spread of SARS-CoV-2 in Nursing Homes
Authors: Schmidt Alec J. , García Yury , Pinheiro Diego , Reichert Thomas A. , Nuño Miriam
Published: January 2022

More than 40% of the deaths recorded in the first wave of the SARS-CoV-2 pandemic were linked to nursing homes. Not only are the residents of long-term care facilities (LTCFs) typically older and more susceptible to endemic infections, the facilities’ high degree of connection to wider communities makes them especially vulnerable to local COVID-19 outbreaks. In 2008, in the wake of the SARS-CoV-1 and MERS epidemics and anticipating an influenza pandemic, we created a stochastic compartmental model to evaluate the deployment of non-pharmaceutical interventions (NPIs) in LTCFs during influenza epidemics. In that model, the most effective NPI by far was a staff schedule consisting of 5-day duty periods with onsite residence, followed by an 4-to-5 day off-duty period with a 3-day quarantine period just prior to the return to work. Unlike influenza, COVID-19 appears to have significant rates of pre-symptomatic transmission. In this study, we modified our prior modeling framework to include new parameters and a set of NPIs to identify and control the degree of pre-symptomatic transmission. We found that infections, deaths, hospitalizations, and ICU utilization were projected to be high and largely irreducible, even with rigorous application of all defined NPIs, unless pre-symptomatic carriers can be identified and isolated at high rates. We found that increasingly rigorous application of NPIs is likely to significantly decrease the peak of infections; but even with complete isolation of symptomatic persons, and a 50% reduction in silent transmission, the attack rate is projected to be nearly 95%.
N′-(3-Bromo-7-oxo-7H-benzo[de]anthracen-9-yl)-N,N-dimethylimidoformamide
Authors: Romanovska Evita , Pučkins Aleksandrs , Grigorjeva Tatjana , Kirilova Elena
Published: January 2022

We report a synthesis of 3-bromo-9-N′-(N,N-dimethylformamidino)benzanthrone in good yield by a condensation reaction of 3-bromo-9-aminobenzanthrone with dimethylformamide in the presence of phosphorous oxychloride. The structure of the synthesized amidine was characterized by FTIR-ATR, NMR experiments, and elemental analysis. The title compound was analyzed by UV-Vis and fluorescence spectroscopy in various organic solvents.
Application of Activated Carbon Banana Peel Coated with Al2O3-Chitosan for the Adsorptive Removal of Lead and Cadmium from Wastewater
Authors: Ramutshatsha-Makhwedzha Denga , Mbaya Richard , Mavhungu Mapula Lucey
Published: January 2022

This study was aimed at evaluating the adsorption capacity of novel banana peel activated carbon (BPAC) modified with Al3O2@chitosan for the removal of cadmium (Cd2+) and lead (Pb2+) from wastewater. Characterization techniques such as X-ray diffraction, scanning electron microscopy, transmission electron microscopy, Fourier transformed infrared (FTIR) spectroscopy, and Brunauer–Emmett–Teller analysis confirmed the synthesized BPAC@Al3O2@chitosan composite material. The univariate approach was used to study the influence of different experimental parameters (such as adsorbent mass, sample pH, and contact time) that affects simultaneous removal of Cd2+ and Pb2+ ions. Kinetic results showed that adsorption favored the pseudo-second-order kinetic model, whereas the adsorption of Cd2+ and Pb2+ was best described by the Langmuir model and the adsorption capacity for Cd2+ and Pb2+ was 46.9 mg g−1 and 57.1 mg g−1, respectively, for monolayer adsorption. It was shown the BPAC composite can be re-used until the third cycle of adsorption–desorption (% Re > 80). Based on the obtained results, it can be concluded that the prepared BPAC@Al3O2@chitosan composite material is cost effective, as it is generated from waste banana peels and can be re-used. In addition, the prepared material was able to remove Cd2+ and Pb2+ up to 99.9%.
Corn Cob Ash versus Sunflower Stalk Ash, Two Sustainable Raw Materials in an Analysis of Their Effects on the Concrete Properties
Authors: Șerbănoiu Adrian Alexandru , Grădinaru Cătălina Mihaela , Muntean Radu , Cimpoeșu Nicanor , Șerbănoiu Bogdan Vasile
Published: January 2022

The increased CO2 emissions determined by the cement industry led to continuous and intensive research on the discovery of sustainable raw materials with cementitious properties. One such raw material category is agricultural waste. This study involved research on the effects of corn cob ash and sunflower stalk ash, respectively, on compressive strength measured after 28 days and 3 months, the flexural and splitting tensile strengths, the resistance to repeated freeze–thaw cycles, and on the resistance to chemical attack of hydrochloric acid of the concrete. A 2.5% and 5% replacement of the cement volume with corn cob ash (CCA) of A and B quality was applied, and with sunflower stalk ash (SSA) at A and B quality, respectively. The obtained results revealed that CCA and SSA decreased the compressive and tensile strength, but led to higher resistance of the concrete on repeated freeze–thaw cycles and to hydrochloric acid. The mixes with 2.5% SSA at A quality obtained the best results regarding splitting the tensile strength and resistance to repeated freeze–thaw cycles, the mixes with 2.5% SSA at B quality led to the highest resistance to hydrochloric acid, and those with 2.5% CCA at A quality led to the best values of compressive strength and flexural tensile strength.
Silver Nanoparticles (AgNPs) in Urea Solution in Laboratory Tests and Field Experiments with Crops and Vegetables
Authors: Jaskulski Dariusz , Jaskulska Iwona , Majewska Joanna , Radziemska Maja , Bilgin Ayla , Brtnicky Martin
Published: January 2022

Nanotechnology and nanomaterials, including silver nanoparticles (AgNPs), are increasingly important in modern science, economics, and agriculture. Their biological activity involves influencing plant health, physiological processes, growth, and yields, although they can also be toxic in the environment. A new fertiliser was made based on a urea solution with a relatively low content of AgNPs obtained by the reduction of silver nitrate V. Laboratory tests were used to assess the effect of a fertiliser solution containing 10 ppm AgNPs on the germination of agricultural plant seeds (barley, peas, oilseed rape) and vegetables (radish, cucumber, lettuce) and its foliar application on chlorophyll content, stomatal conductance, and seedling biomass. Field experiments were conducted to assess the effect that a foliar application of 15 ppm AgNPs in working liquid had on physiological plant parameters and yields of rape and cucumber. The AgNPs in the tested fertiliser reduced infestation of the germinating seeds by pathogens and positively affected the physiological processes, productivity, and yields of plants. Plant response depended on plant species and habitat conditions. Reduced pathogen infestation of seeds, higher germination energy, increased chlorophyll content and stomatal conductance, and higher seedling masses all occurred under the influence of AgNPs, mainly in oilseed rape and cucumber, and especially under thermal stress. The beneficial effect of AgNPs on the yield of these plants occurred in years of unfavourable weather conditions. The positive agricultural test results, especially under stress conditions, indicate that fertiliser produced with AgNPs as an ingredient may reduce the use of pesticides and highly concentrated mineral fertilisers. Such a fertiliser is fully in line with the idea of sustainable agriculture. However, research on the effects that AgNPs and fertiliser have on the environment and humans should continue.
Evaluation of Disposal Stability for Cement Solidification of Lime Waste
Authors: Shon Jong-Sik , Lee Hyun-Kyu , Kim Gi-Yong , Kim Tack-Jin , Ahn Byung-Gil
Published: January 2022

The Korea Atomic Energy Research Institute (KAERI) obtains UO2 powder using the ammonium uranyl carbonate (AUC) wet process. Hydrated lime (Ca(OH)2) is used to neutralize liquid wastes produced from the AUC process, and the resulting byproduct is known as lime waste. The purpose of this study is to determine optimum operating conditions for cementation of radioactive lime waste produced from the AUC process, and to evaluate the structural stability and leaching stability of cement waste form. The waste acceptance criteria (WAC) of a waste disposal facility in Korea were used to evaluate the cement waste form samples. The maximum lime waste content guaranteeing the shape stability of cement waste form was found to be 80 wt.% or less. Considering the economic feasibility and error of the cementation process, the optimum operating conditions were achieved at a lime waste content of 75 wt.% and a water-to-cement (w/c) ratio of 2.0. The compressive strength of cement waste form samples prepared under optimal operating conditions was 61.4, 76.3, and 61.0 kgf/cm2 after the thermal cycling test, water immersion test, and irradiation, respectively, satisfying the compressive strength of 35.2 kgf/cm2 specified in WAC. A leaching test was performed on the samples, and the leachability indexes (LX) of Cs, Sr, and Co nuclides were 7.63, 8.02, and 10.89, respectively, which are all higher than the acceptance criterion of 6. The results showed that the cement waste forms prepared under optimal operating conditions satisfied the WAC in terms of structural stability and leaching stability. As such, the proposed cement solidification method for lime waste disposal can be effective in solidifying lime waste powder produced during the neutralization of liquid wastes in the AUC process.
A Time-Modulated Transparent Nonlinear Active Metasurface for Spatial Frequency Mixing
Authors: Wang Luyi , Shi Hongyu , Peng Gantao , Yi Jianjia , Dong Liang , Zhang Anxue , Xu Zhuo
Published: January 2022

In this article, a time-modulated transparent nonlinear active metasurface loaded with varactor diodes was proposed to realize spatial electromagnetic (EM) wave frequency mixing. The nonlinear transmission characteristic of the active metasurface was designed and measured under time-modulated biasing signals. The transmission phase can be continuously controlled across a full 360° range at 5 GHz when the bias voltage of the varactor diodes changes from 0 V to 25.5 V, while the transmission amplitude is between −2.1 dB to −2.7 dB. By applying the bias voltage in time-modulated sequences, frequency mixing can be achieved. Due to the nonlinearity of the transmission amplitude and transmission phase of the metasurface versus a time-modulated bias voltage, harmonics of the fundamental mode were observed using an upper triangle bias voltage. Furthermore, with a carefully designed bias voltage sequence, unwanted higher order harmonics were suppressed. The proposed theoretical results are validated with the measured results.
Study of Cutting Power and Power Efficiency during Straight-Tooth Cylindrical Milling Process of Particle Boards
Authors: Li Rongrong , Yao Qian , Xu Wei , Li Jingya , Wang Xiaodong (Alice)
Published: January 2022

The cutting power consumption of milling has direct influence on the economic benefits of manufacturing particle boards. The influence of the milling parameters on the cutting power were investigated in this study. Experiments and data analyses were conducted based on the response surface methodology. The results show that the input parameters had significant effects on the cutting power. The high rake angle reduced the cutting force. Thus, the cutting power decreased with the increase in the rake angle and the cutting energy consumption was also reduced. The cutting power increased with the rotation speed of the main shaft and the depth of milling induced the impact resistance between the milling tool and particle board and the material removal rate. The p-values of the created models and input parameters were less than 0.05, which meant they were significant for cutting power and power efficiency. The depth of milling was the most important factor, followed by the rotation speed of the main shaft and then the rake angle. Due to the high values of R2 of 0.9926 and 0.9946, the quadratic models were chosen for creating the relationship between the input parameters and response parameters. The predicted values of cutting power and power efficiency were close to the actual values, which meant the models could perform good predictions. To minimize the cutting power and maximize the power efficiency for the particle board, the optimized parameters obtained via the response surface methodology were 2°, 6991.7 rpm, 1.36 mm for rake angle, rotation speed of the main shaft and depth of milling, respectively. The model further predicted that the optimized parameters combination would achieve cutting power and power efficiency values of 52.4 W and 11.9%, respectively, with the desirability of 0.732. In this study, the influence of the input parameters on the cutting power and power efficiency are revealed and the created models were useful for selecting the milling parameters for particle boards, to reduce the cutting power.
Experimental Study on Strength Enhancement of Expansive Grout
Authors: Wang Di , Ye Yicheng , Yao Nan , Liu Yiming , Deng Xingmin
Published: January 2022

Adding an expansive agent to ordinary grout can cause an expansion in volume, but also reduces its strength. In order to improve the strength of expansive grout, quartz sand is used as the strength enhancement additive. In this study, the expansion behavior and mechanical properties of the expansive grout with quartz sand are explored, through expansion development monitoring, uniaxial compression strength (UCS), acoustic emission (AE), SEM and XRD test methods. The results showed that: (1) The final expansion ratio and expansion development of the samples are related to the use of an expansive agent, but not affected by quartz sand. With the increase in expansion agent content, the average expansion ratios of the samples are 0.03%, 0.16%, 0.67%, 1.06% and 1.48%; (2) The UCS of the samples decreases with the increase in expansive agent content but increases with the increase in quartz sand content. Compared with no quartz sand, and with the increase in quartz sand content, the average strength of the samples increased by 10.51%, 29.88%, and 37.92%; (3) Quartz sand does not effectively participate in the hydration reaction, but it can effectively enhance the strength of the expansive grout without affecting its volume expansion, which makes it an ideal expansive grout strength enhancement additive.
Combining an Intensive Green Roof with Seismic Retrofitting of Typical Reinforced Concrete Buildings in Israel
Authors: Pushkar Svetlana , Halperin Ido , Ribakov Yuri
Published: January 2022

This study suggests an intensive green roof as part of a sustainable and hazard-resistant conceptual design for the retrofitting of old buildings in Israel. The roof is suggested to be built with waste-based materials. A five-story reinforced concrete residential building was retrofitted with: Case 1: concrete wall strengthening (CWS)-conventional concrete + conventional green roof; Case 2: CWS-waste-included concrete + waste-based green roof; Case 3: seismic isolation columns (SIC)-conventional concrete + conventional green roof; and Case 4: SIC-waste-included concrete + waste-based green roof. Palekastro, Nuweiba, Tabas, and Erzincan ground motions were used for a structural dynamic time-history analysis of the retrofitted buildings. Life cycle assessments of cases 1–4 were performed using ReCiPe 2016 midpoint and endpoint evaluations. A two-stage analysis of variance (ANOVA) was used to analyze the ReCiPe endpoint results. According to the seismic results, Case 3 and Case 4 were much more preferable to Case 1 and Case 2, whereas according to the environmental evaluations, Case 4 was the most preferable to the other cases.
Reducing the Core Losses of Fe-Si-B Amorphous Alloy Ribbons by High Cooling Rate Planar Flow Casting
Authors: Li Deren , Wang Wenjun , Liu Tiancheng , Li Lijun , Lu Zhichao
Published: January 2022

In the planar flow casting (PFC) process, the cooling rate significantly affects the structure and properties of a cast ribbon. The influence of the thermal conductivity of the cooling wheel substrate on cooling rate was simulated by a numerical method, and it is shown that a higher thermal conductivity of the cooling wheel substrate leads to a higher cooling rate in the PFC process. Two copper-beryllium (Cu-2Be) rings with thermal conductivities of 175.3 W/m·K and 206.5 W/m·K were manufactured and installed onto a wheel core as the substrate of the cooling wheel. The effects of cooling rate on the soft magnetic properties of Fe-Si-B amorphous ribbons were investigated by pragmatic ribbon casting. The results show that the increment in the thermal conductivity of the cooling wheel substrate from 175.3 W/m·K to 206.5 W/m·K lowered the coercive force of amorphous ribbon from 2.48 A/m to 1.92 A/m and reduced the core losses at 1.4 T and 50 Hz by up to 22.1%.
Stochastic Magnetization Switching in Rapidly Solidified (Co0.94Fe0.06)72.5Si12.5B15 Amorphous Submicronic Wires
Authors: Corodeanu Sorin , Hlenschi Costică , Rotărescu Cristian , Chiriac Horia , Lupu Nicoleta , Óvári Tibor-Adrian
Published: January 2022

Submicrometric magnetic amorphous wires are good candidates for future development of miniaturized sensors and magnetic logic applications. Here we report the results of an in-depth investigation of magnetization switching in rapidly solidified nearly zero magnetostrictive (Co0.94Fe0.06)72.5Si12.5B15 amorphous samples with diameters of the actual magnetic wires between 300 and 450 nm. All samples were found to be magnetically bistable, displaying characteristic rectangular hysteresis loops. This shows that magnetization reversal occurs through the depinning and subsequent propagation of a magnetic domain wall, whose velocity depends on the applied field and on the sample dimensions. The results of this study reveal stochastic nonlinear dependencies of both the magnetic switching field and the domain wall velocity on the sample diameter. The analysis of the potential causes, which include nonlinear residual stresses, fluctuations in wire dimensions (metal and glass), and competing magnetic anisotropies of different origins, show that a combination of all three factors could lead to the observed stochastic behavior. Calculated values of the switching field, which consider only changes in the wire dimensions, indicate that such influence alone cannot account for the strong nonlinearities. The results are important for the applications of such ultrathin cylindrical magnetic amorphous wires.