bluuebunny/crossref_metadata_2025 huggingface dataset:
Dry and MQL Milling of AISI 1045 Steel with Vegetable and Mineral-Based Fluids
Authors: Baldin Vitor , da Silva Leonardo Rosa Ribeiro , Davis Rahul , Jackson Mark James , Amorim Fred Lacerda , Houck Celso Ferraz , Machado Álisson Rocha
Published: April 2023

The use of mineral-based cutting fluids in machining has the drawback of affecting the environment and industries are under pressures to reduce its use in favor of cleaner productions. In this regard, the vegetal-based cutting fluids can be a superior alternative, provided they improve the technical outcomes. In the milling process, dry cutting is commonly performed, however, the application of cutting fluids using the minimum quantity of lubricant (MQL) method has proven advantageous when compared with dry machining. Furthermore, in the midst of the availability of several cutting fluids in the market, the testing of their individual performance can ascertain their potential and effectiveness for a particular application. This study examined the performances of two vegetable-based and one mineral-based oils applied by the MQL method, followed by their comparison with dry cutting amid end milling of AISI 1045 steel with TiAlN-coated cemented carbide inserts. The cutting temperature, machining forces, power consumption, workpiece surface roughness, tool life, and tool wear mechanisms were chosen as the output parameters. The experiments were conducted using two cutting speeds (150 and 200 m/min) and feed rates (0.07 and 0.14 mm/tooth), and constant axial (1 mm) and radial depths of the cut (25 mm). The temperature was measured using a K-type thermocouple soldered to the part and an infrared camera. The power was monitored with a Fluke 435 energy analyzer, and the machining force components with a Kistler dynamometer. The worn inserts were inspected under a scanning electron microscope (SEM) to analyze the tool wear mechanism. The MQL-assisted application of the cutting fluids notably lowered the cutting temperature and increased the tools’ lives. However, the cutting fluids did not reflect any significant effect on the machining force, power consumption, or surface roughness. Among all the analyzed cutting conditions, the abrasive wear mechanism dominated, damaging the cutting edges, flank, and rake surfaces of the cutting tools. In addition, adhesive and diffusion wear mechanisms were also observed.
Loss Analysis of a Transonic Rotor with a Differential Approach to Entropy Generation
Authors: Ma Jingyuan , Lin Feng
Published: April 2023

The loss mechanism of transonic axial compressors is a long-standing problem that involves almost all types of entropy generation in fluid flows, such as skin friction, shock waves, shear flows, corner separation, and tip vortices. Primarily, sources need to be identified and quantitative comparisons of their contributions need to be made. For such determination, we propose herein a differential approach to entropy generation, called the “differential approach”. Two case studies are analyzed to determine the applicability of this approach: (1) laminar and turbulent incompressible flows in straight circular ducts and (2) turbulent compressible flows in convergent-and-divergent nozzles with shock waves. The results lead to the following conclusions: (a) Qualitatively, the differential approach works well, and the quantified measure is reliable if it is calculated with quality meshes and a suitable turbulence model. This means that the differential approach can be a good tool for predesign optimization. (b) When shocks occur within flow fields, the shock-induced boundary-layer separation can generate more loss than the shock loss alone. Subsequently, the differential approach is applied to complex flows in the NASA Rotor 67, which is a well-known bench-test transonic rotor. The results show that the differential approach not only determines the local losses and associates the source of losses with the flow structures but also qualitatively compares and identifies the largest contributors. These results provide a theoretical foundation for optimizing rotor design and enhancing stability.
Novel Algorithms with Inertial Techniques for Solving Constrained Convex Minimization Problems and Applications to Image Inpainting
Authors: Hanjing Adisak , Suantai Suthep
Published: April 2023

In this paper, we propose two novel inertial forward–backward splitting methods for solving the constrained convex minimization of the sum of two convex functions, φ1+φ2, in Hilbert spaces and analyze their convergence behavior under some conditions. For the first method (iFBS), we use the forward–backward operator. The step size of this method depends on a constant of the Lipschitz continuity of ∇φ1, hence a weak convergence result of the proposed method is established under some conditions based on a fixed point method. With the second method (iFBS-L), we modify the step size of the first method, which is independent of the Lipschitz constant of ∇φ1 by using a line search technique introduced by Cruz and Nghia. As an application of these methods, we compare the efficiency of the proposed methods with the inertial three-operator splitting (iTOS) method by using them to solve the constrained image inpainting problem with nuclear norm regularization. Moreover, we apply our methods to solve image restoration problems by using the least absolute shrinkage and selection operator (LASSO) model, and the results are compared with those of the forward–backward splitting method with line search (FBS-L) and the fast iterative shrinkage-thresholding method (FISTA).
Calibration-Based Mean Estimators under Stratified Median Ranked Set Sampling
Authors: Shahzad Usman , Ahmad Ishfaq , Alshahrani Fatimah , Almanjahie Ibrahim M. , Iftikhar Soofia
Published: April 2023

Using auxiliary information, the calibration approach modifies the original design weights to enhance the mean estimates. This paper initially proposes two families of estimators based on an adaptation of the estimators presented by recent researchers, and then, it presents a new family of calibration estimators with the set of some calibration constraints under stratified median ranked set sampling (MRSS). The result has also been implemented to the situation of two-stage stratified median ranked set sampling (MRSS). To best of our knowledge, we are presenting for the first time calibration-based mean estimators under stratified MRSS, so the performance evaluation is made between adapted and proposed estimators on behalf of the simulation study with real and artificial datasets. For real-world data or applications, we use information on the body mass index (BMI) of 800 people in Turkey in 2014 as a research variable and age as an auxiliary variable.
Advances in Research on the Relationship between Vaginal Microbiota and Adverse Pregnancy Outcomes and Gynecological Diseases
Authors: Zhao Fuju , Hu Xianyang , Ying Chunmei
Published: April 2023

The human microbiota inhabiting different parts of the body has been shown to have a significant impact on human health, with the gut microbiota being the most extensively studied in relation to disease. However, the vaginal microbiota is also an essential commensal microbiota in the female body that plays a crucial role in female health. Despite receiving less attention than gut microbiota, its importance in regulating reproductive immunity and its complex dynamic properties have been increasingly recognized in recent years. Advances in research on the relationship between vaginal microbiota and pregnancy outcomes & gynecological diseases in women have shed light on the importance of maintaining a healthy vaginal microbiota. In this review, we aim to compile recent developments in the study of the vaginal microbial ecosystem and its role in female health and reproductive outcomes. We provide a comprehensive account of the normal vaginal microbiota, the association between the vaginal microbiota and pregnancy outcomes, and the impact of the vaginal microbiota on gynecological diseases in women. By reviewing recent research, we hope to contribute to the advancement of academic medicine’s understanding of the vaginal microbiota’s importance in female health. We also aim to raise awareness among healthcare professionals and the general public of the significance of maintaining a healthy vaginal microbiota for better reproductive health and the prevention of gynecological diseases.
The Survival of Psychobiotics in Fermented Food and the Gastrointestinal Tract: A Review
Authors: Cichońska Patrycja , Kowalska Ewa , Ziarno Małgorzata
Published: April 2023

In recent years, scientists have been particularly interested in the gut–brain axis, as well as the impact of probiotics on the nervous system. This has led to the creation of the concept of psychobiotics. The present review describes the mechanisms of action of psychobiotics, their use in food products, and their viability and survival during gastrointestinal passage. Fermented foods have a high potential of delivering probiotic strains, including psychobiotic ones. However, it is important that the micro-organisms remain viable in concentrations ranging from about 106 to 109 CFU/mL during processing, storage, and digestion. Reports indicate that a wide variety of dairy and plant-based products can be effective carriers for psychobiotics. Nonetheless, bacterial viability is closely related to the type of food matrix and the micro-organism strain. Studies conducted in laboratory conditions have shown promising results in terms of the therapeutic properties and viability of probiotics. Because human research in this field is still limited, it is necessary to broaden our understanding of the survival of probiotic strains in the human digestive tract, their resistance to gastric and pancreatic enzymes, and their ability to colonize the microbiota.
Improved Chrysin Production by a Combination of Fermentation Factors and Elicitation from Chaetomium globosum
Authors: Kamat Siya , Kumari Madhuree , Sajna Kuttuvan , Singh Sandeep , Kaushalendra  , Kumar Ajay , Jayabaskaran C.
Published: April 2023

Flavonoids encompass a heterogeneous group of secondary metabolites with exceptional health benefits. Chrysin, a natural dihydroxyflavone, possesses numerous bioactive properties, such as anticancer, antioxidative, antidiabetic, anti-inflammatory, etc. However, using traditional sources of chrysin involves extracting honey from plants, which is non-scalable, unsustainable, and depends on several factors, including geography, climatic conditions, and the season, which limits its production at a larger scale. Recently, microbial production of desirable metabolites has garnered attention due to the cost-effectiveness, easy scale-up, sustainability, and low emission of waste. We previously reported for the first time the chrysin-producing marine endophytic fungus Chaetomium globosum, associated with a marine green alga. To extend our understanding of chrysin biosynthesis in C. globosum, in the present study, we have assessed the presence of flavonoid pathway intermediates in C. globosum extracts using LC-MS/MS. The presence of several key metabolites, such as dihydrokaempferol, chalcone, galangin, baicalein, chrysin, p-Coumaroyl-CoA, and p-Cinnamoyl-CoA, indicates the role of flavonoid biosynthesis machinery in the marine fungus. Further, we have aimed to enhance the production of chrysin with three different strategies: (1) optimizing the fermentation parameters, namely, growth medium, incubation time, pH, and temperature; (2) feeding key flavonoid pathway intermediates, i.e., phenylalanine and cinnamic acid; (3) elicitation with biotic elicitors, such as polysaccharide, yeast extract, and abiotic elicitors that include UV radiation, salinity, and metal stress. The combined effect of the optimized parameters resulted in a 97-fold increase in the chrysin yield, resulting in a fungal cell factory. This work reports the first approach for enhanced production of chrysin and can serve as a template for flavonoid production enhancement using marine endophytic fungi.
Phytochemicals of Withania somnifera as a Future Promising Drug against SARS-CoV-2: Pharmacological Role, Molecular Mechanism, Molecular Docking Evaluation, and Efficient Delivery
Authors: Ramli Suaidah , Wu Yuan Seng , Batumalaie Kalaivani , Guad Rhanye Mac , Choy Ker Woon , Kumar Ashok , Gopinath Subash C. B. , Rahman Sarker Md. Moklesur , Subramaniyan Vetriselvan , Sekar Mahendran , Fuloria Neeraj Kumar , Fuloria Shivkanya , Chinni Suresh V. , Ramachawolran Gobinath
Published: April 2023

Coronavirus disease (COVID-19) has killed millions of people since first reported in Wuhan, China, in December 2019. Intriguingly, Withania somnifera (WS) has shown promising antiviral effects against numerous viral infections, including SARS-CoV and SARS-CoV-2, which are contributed by its phytochemicals. This review focused on the updated testing of therapeutic efficacy and associated molecular mechanisms of WS extracts and their phytochemicals against SARS-CoV-2 infection in preclinical and clinical studies with the aim to develop a long-term solution against COVID-19. It also deciphered the current use of the in silico molecular docking approach in developing potential inhibitors from WS targeting SARS-CoV-2 and host cell receptors that may aid the development of targeted therapy against SARS-CoV-2 ranging from prior to viral entry until acute respiratory distress syndrome (ARDS). This review also discussed nanoformulations or nanocarriers in achieving effective WS delivery to enhance its bioavailability and therapeutic efficacy, consequently preventing the emergence of drug resistance, and eventually therapeutic failure.
The Effect of the Microalgae Chlorella vulgaris on the Gut Microbiota of Juvenile Nile Tilapia (Oreochromis niloticus) Is Feeding-Time Dependent
Authors: Huang Zhicheng , Gao Jinyan , Peng Chunyan , Song Jingjing , Xie Zongsheng , Jia Jixin , Li Haochen , Zhao Shumiao , Liang Yunxiang , Gong Bin
Published: April 2023

Chlorella vulgaris is one of the most commonly used microalgae in aquaculture feeds. It contains high concentrations of various kinds of nutritional elements that are involved in the physiological regulation of aquaculture animals. However, few studies have been conducted to illustrate their influence on the gut microbiota in fish. In this work, the gut microbiota of Nile tilapia (Oreochromis niloticus) (average weight is 6.64 g) was analyzed by high-throughput sequencing of the 16S rRNA gene after feeding with 0.5% and 2% C. vulgaris additives in diets for 15 and 30 days (average water temperature was 26 °C). We found that the impact of C. vulgaris on the gut microbiota of Nile tilapia was feeding-time dependent. Only by feeding for 30 days (not 15 days) did the addition of 2% C. vulgaris to diets significantly elevate the alpha diversity (Chao1, Faith pd, Shannon, Simpson, and the number of observed species) of the gut microbiota. Similarly, C. vulgaris exerted a significant effect on the beta diversity (Bray–Curtis similarity) of the gut microbiota after feeding for 30 days (not 15 days). During the 15-day feeding trial, LEfSe analysis showed that Paracoccus, Thiobacillus, Dechloromonas, and Desulfococcus were enriched under 2% C. vulgaris treatment. During the 30-day feeding trial, Afipia, Ochrobactrum, Polymorphum, Albidovulum, Pseudacidovorax, and Thiolamprovum were more abundant in 2% C. vulgaris-treated fish. C. vulgaris promoted the interaction of gut microbiota in juvenile Nile tilapia by increasing the abundance of Reyranella. Moreover, during the feeding time of 15 days, the gut microbes interacted more closely than those during the feeding time of 30 days. This work will be valuable for understanding how C. vulgaris in diets impacts the gut microbiota in fish.
Continuous Production of Ethanol, 1-Butanol and 1-Hexanol from CO with a Synthetic Co-Culture of Clostridia Applying a Cascade of Stirred-Tank Bioreactors
Authors: Bäumler Miriam , Burgmaier Veronika , Herrmann Fabian , Mentges Julian , Schneider Martina , Ehrenreich Armin , Liebl Wolfgang , Weuster-Botz Dirk
Published: April 2023

Syngas fermentation with clostridial co-cultures is promising for the conversion of CO to alcohols. A CO sensitivity study with Clostridium kluyveri monocultures in batch operated stirred-tank bioreactors revealed total growth inhibition of C. kluyveri already at 100 mbar CO, but stable biomass concentrations and ongoing chain elongation at 800 mbar CO. On/off-gassing with CO indicated a reversible inhibition of C. kluyveri. A continuous supply of sulfide led to increased autotrophic growth and ethanol formation by Clostridium carboxidivorans even at unfavorable low CO concentrations. Based on these results, a continuously operated cascade of two stirred-tank reactors was established with a synthetic co-culture of both Clostridia. An amount of 100 mbar CO and additional sulfide supply enabled growth and chain elongation in the first bioreactor, whereas 800 mbar CO resulted in an efficient reduction of organic acids and de-novo synthesis of C2-C6 alcohols in the second reactor. High alcohol/acid ratios of 4.5–9.1 (w/w) were achieved in the steady state of the cascade process, and the space-time yields of the alcohols produced were improved by factors of 1.9–5.3 compared to a batch process. Further improvement of continuous production of medium chain alcohols from CO may be possible by applying less CO-sensitive chain-elongating bacteria in co-cultures.
Computational Insights into the Dynamic Structural Features and Binding Characteristics of Recombinase UvsX Compared with RecA
Authors: Pan Yue , Xie Ningkang , Zhang Xin , Yang Shuo , Lv Shaowu
Published: April 2023

RecA family recombinases are the core enzymes in the process of homologous recombination, and their normal operation ensures the stability of the genome and the healthy development of organisms. The UvsX protein from bacteriophage T4 is a member of the RecA family recombinases and plays a central role in T4 phage DNA repair and replication, which provides an important model for the biochemistry and genetics of DNA metabolism. UvsX shares a high degree of structural similarity and function with RecA, which is the most deeply studied member of the RecA family. However, the detailed molecular mechanism of UvsX has not been resolved. In this study, a comprehensive all-atom molecular dynamics simulation of the UvsX protein dimer complex was carried out in order to investigate the conformational and binding properties of UvsX in combination with ATP and DNA, and the simulation of RecA was synchronized with the property comparison learning for UvsX. This study confirmed the highly conserved molecular structure characteristics and catalytic centers of RecA and UvsX, and also discovered differences in regional conformation, volatility and the ability to bind DNA between the two proteins at different temperatures, which would be helpful for the subsequent understanding and application of related recombinases.
Phytochemical Analysis of Anastatica hierochuntica and Aerva javanica Grown in Qatar: Their Biological Activities and Identification of Some Active Ingredients
Authors: Thotathil Vandana , Sidiq Naheed , Fakhroo Ameena , Sreerama Lakshmaiah
Published: April 2023

Plant-derived compounds and their extracts are known to exhibit chemo preventive (antimicrobial, antioxidant and other) activities. The levels of such chemo preventive compounds vary depending on environmental factors, including the regions where they grow. Described in this study are: (i) a phytochemical analysis of the two plants grown in the desert environment of Qatar, viz., Anastatica hierochuntica and Aerva javanica; (ii) the antibacterial, antifungal and antioxidant activities of various solvent extracts of these plants; (iii) a report on the isolation of several pure compounds from these plants. The phytochemical screening indicated the presence of glycosides, tannins, flavonoids, terpenoids, saponins, phenol and anthraquinones in various extracts of each of the plants. Antibacterial and antioxidant activities were studied using agar diffusion and DPPH methods, respectively. The extracts of Anastatica hierochuntica as well as Aerva javanica inhibit the growth of both gram-positive and gram-negative bacterial species. Various extracts of the two plants also exhibited higher or similar antioxidant activities as those of the standard antioxidants, α-tocopherol and ascorbic acid. The extracts of these plants were further purified by HPLC and characterized by IR and NMR techniques. This process has led to identification of β-sitosterol, campesterol and methyl-9-(4-(3,4-dihydroxy-1′-methyl-5′-oxocyclohexyl)-2-hydroxycyclohexyl)nonanoate from Anastatica hierochuntica, and lupenone, betulinic acid, lupeol acetate and persinoside A and B from Aerva javanica. The results reported herein suggests that Anastatica hierochuntica and Aerva javanica are potent sources of phytomedicines.
Cell-Penetrating Peptides for Use in Development of Transgenic Plants
Authors: Liu Betty Revon , Chen Chi-Wei , Huang Yue-Wern , Lee Han-Jung
Published: April 2023

Genetically modified plants and crops can contribute to remarkable increase in global food supply, with improved yield and resistance to plant diseases or insect pests. The development of biotechnology introducing exogenous nucleic acids in transgenic plants is important for plant health management. Different genetic engineering methods for DNA delivery, such as biolistic methods, Agrobacterium tumefaciens-mediated transformation, and other physicochemical methods have been developed to improve translocation across the plasma membrane and cell wall in plants. Recently, the peptide-based gene delivery system, mediated by cell-penetrating peptides (CPPs), has been regarded as a promising non-viral tool for efficient and stable gene transfection into both animal and plant cells. CPPs are short peptides with diverse sequences and functionalities, capable of agitating plasma membrane and entering cells. Here, we highlight recent research and ideas on diverse types of CPPs, which have been applied in DNA delivery in plants. Various basic, amphipathic, cyclic, and branched CPPs were designed, and modifications of functional groups were performed to enhance DNA interaction and stabilization in transgenesis. CPPs were able to carry cargoes in either a covalent or noncovalent manner and to internalize CPP/cargo complexes into cells by either direct membrane translocation or endocytosis. Importantly, subcellular targets of CPP-mediated nucleic acid delivery were reviewed. CPPs offer transfection strategies and influence transgene expression at subcellular localizations, such as in plastids, mitochondria, and the nucleus. In summary, the technology of CPP-mediated gene delivery provides a potent and useful tool to genetically modified plants and crops of the future.
Effect of Electrospinning Network Instead of Polymer Network on the Properties of PDLCs
Authors: Zhao Yuzhen , Lang Tingting , Li Chaonian , Yin Wenbo , Sun Yitian , Yao Ruijuan , Ma Cheng , Shi Zuhui , Wang Dong , Miao Zongcheng
Published: April 2023

In this study, polymer-dispersed liquid crystal (PDLC) membranes were prepared by combining prepolymer, liquid crystal, and nanofiber mesh membranes under UV irradiation. EM, POM, and electro-optic curves were then used to examine the modified polymer network structure and the electro-optical properties of these samples. As a result, the PDLCs with a specific amount of reticular nanofiber films had considerably improved electro-optical characteristics and antiaging capabilities. The advancement of PDLC incorporated with reticulated nanofiber films, which exhibited a faster response time and superior electro-optical properties, would greatly enhance the technological application prospects of PDLC-based smart windows, displays, power storage, and flexible gadgets.
Synthesis, Structural Determination, and Antifungal Activity of Novel Fluorinated Quinoline Analogs
Authors: Sun Xinpeng , Yu Wei , Min Lijing , Han Liang , Hua Xuewen , Shi Jianjun , Sun Nabo , Liu Xinghai
Published: April 2023

A series of new fluorinated quinoline analogs were synthesized using Tebufloquin as the lead compound, 2-fluoroaniline, ethyl 2-methylacetoacetate, and substituted benzoic acid as raw materials. Their structures were confirmed by 1H NMR, 13C NMR, and HRMS. The compound 8-fluoro-2,3-dimethylquinolin-4-yl 4-(tert-butyl)benzoate (2b) was further determined by X-ray single-crystal diffraction. The antifungal activity was tested at 50 μg/mL, and the bioassay results showed that these quinoline derivatives had good antifungal activity. Among them, compounds 2b, 2e, 2f, 2k, and 2n exhibited good activity (>80%) against S. sclerotiorum, and compound 2g displayed good activity (80.8%) against R. solani.
The Role of Substrate Surface Geometry in the Photo-Electrochemical Behaviour of Supported TiO2 Nanotube Arrays: A Study Using Electrochemical Impedance Spectroscopy (EIS)
Authors: De Pasquale Luana , Tavella Francesco , Longo Victor , Favaro Marco , Perathoner Siglinda , Centi Gabriele , Ampelli Claudio , Genovese Chiara
Published: April 2023

Highly ordered TiO2 nanotube (NT) arrays grown on Ti mesh and Ti foil were successfully prepared by a controlled anodic oxidation process and tested for water photo-electrolysis. Electrochemical impedance spectroscopy (EIS), combined with other electrochemical techniques (cyclic voltammetry and chronoamperometry) in tests performed in the dark and under illumination conditions, was used to correlate the photoactivity to the specific charge transfer resistances associated with a 3D (mesh) or 2D (foil) geometry of the support. The peculiar structure of the nanotubes in the mesh (with better light absorption and faster electron transport along the nanotubes) strongly impacts the catalytic performances under illumination. H2 production and current density in water photo-electrolysis were over three times higher with the TiO2NTs/Ti mesh, compared to the foil in the same conditions. The results obtained by the EIS technique, used here for the first time to directly compare TiO2 nanotubes on two different supports (Ti foil and Ti mesh), led to a better understanding of the electronic properties of TiO2 nanotubes and the effect of a specific support on its photocatalytic properties.
Recent Advances in the Synthesis of Propargyl Derivatives, and Their Application as Synthetic Intermediates and Building Blocks
Authors: Abonia Rodrigo , Insuasty Daniel , Laali Kenneth K.
Published: April 2023

The propargyl group is a highly versatile moiety whose introduction into small-molecule building blocks opens up new synthetic pathways for further elaboration. The last decade has witnessed remarkable progress in both the synthesis of propargylation agents and their application in the synthesis and functionalization of more elaborate/complex building blocks and intermediates. The goal of this review is to highlight these exciting advances and to underscore their impact.
Antioxidative Activities of Micronized Solid-State Cultivated Hericium erinaceus Rich in Erinacine A against MPTP-Induced Damages
Authors: Hsu Chun-Hsien , Liao En-Chih , Chiang Win-Chin , Wang Kai-Lee
Published: April 2023

The Lion’s mane mushroom (Hericium erinaceus, HE) is a traditional medical mushroom with high nutritional and economic value. HE possesses anticancer, antimicrobial, antioxidant, immunomodulating, neurotrophic, and neuroprotective activities. The present study evaluated the protection and antioxidative activities of micronized mycelium of HE (HEM) in mice treated with 1-methyl-4-phenylpyridinium (MPTP). HEM was cultivated via solid-state fermentation and micronized using cell wall-breaking technology to increase its bioavailability when ingested. Erinacine A, the bioactive compound in the HEM, played a pivotal role in antioxidant defense. We found that micronized HEM could recover the dopamine level in the mice striatum in a dose-dependent manner that had been greatly reduced during MPTP treatment. Moreover, the malondialdehyde (MDA) and carbonyl levels were reduced in the livers and brains of the MPTP + HEM-treated groups compared with the MPTP group. Additionally, antioxidant enzyme activities, including catalase, superoxide dismutase (SOD), glucose-6-phosphate dehydrogenase (G6PDH), and glutathione reductase (GRd), were elevated after the administration of HEM in MPTP-treated mice in a dose-dependent manner. Taken together, our data indicate that HEM cultivated via solid-state fermentation and processed with cell wall-breaking technology showed an excellent antioxidant efficacy.
A Novel Derivative of Curcumol, HCL-23, Inhibits the Malignant Phenotype of Triple-Negative Breast Cancer and Induces Apoptosis and HO-1-Dependent Ferroptosis
Authors: Zhao Peng , Song Hui , Gao Futian , Chen Liang , Qiu Jianfei , Jin Jun , Pan Chaolan , Tang Yunyan , Chen Meijun , Pan Yang , Li Yanmei , Huang Liejun , Yang Jue , Hao Xiaojiang
Published: April 2023

Triple-negative breast cancer (TNBC) is the most aggressive molecular subtype of breast cancer. Curcumol, as a natural small molecule compound, has potential anti-breast cancer activity. In this study, we chemically synthesized a derivative of curcumol, named HCL-23, by structural modification and explored its effect on and underlying mechanism regarding TNBC progression. MTT and colony formation assays demonstrated that HCL-23 significantly inhibited TNBC cells proliferation. HCL-23 induced G2/M phase cell cycle arrest and repressed the capability of migration, invasion, and adhesion in MDA-MB-231 cells. RNA-seq results identified 990 differentially expressed genes including 366 upregulated and 624 downregulated genes. Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and Gene Set Enrichment Analysis (GSEA) revealed that these differentially expressed genes were obviously enriched in adhesion, cell migration, apoptosis, and ferroptosis. Furthermore, HCL-23 induced apoptosis via the loss of mitochondrial membrane potential and the activation of the caspase family in TNBC cells. In addition, HCL-23 was verified to trigger ferroptosis through increasing cellular reactive oxygen species (ROS), labile iron pool (LIP), and lipid peroxidation levels. Mechanistically, HCL-23 markedly upregulated the expression of heme oxygenase 1 (HO-1), and the knockdown of HO-1 could attenuate ferroptosis induced by HCL-23. In animal experiments, we found that HCL-23 inhibited tumor growth and weight. Consistently, the upregulation of Cleaved Caspase-3, Cleaved PARP, and HO-1 expression was also observed in tumor tissues treated with HCL-23. In summary, the above results suggest that HCL-23 can promote cell death through activating caspases-mediated apoptosis and HO-1-dependent ferroptosis in TNBC. Therefore, our findings provide a new potential agent against TNBC.
A Novel Sulfonamide, Molecularly Imprinted, Upconversion Fluorescence Probe Prepared by Pickering Emulsion Polymerization and Its Adsorption and Optical Sensing Performance
Authors: Pan Qidi , Gao Zhe , Meng He , Guo Xianghua , Zhang Meitian , Tang Yiwei
Published: April 2023

A novel, molecularly imprinted, upconversion fluorescence probe (UCNP@MIFP) for sulfonamide sensing was fabricated by Pickering emulsion polymerization using UCNP@SiO2 particles as the stabilizer and sulfamethazine/sulfamerazine as the co-templates. The synthesis conditions of the UCNP@MIFP were optimized, and the synthesized probe was characterized by scanning electron microscopy, Fourier transform infrared spectrometer, thermogravimetric analyzer, and fluorescence spectrometer. The UCNP@MIFPs showed a good adsorption capacity and a fast kinetic feature for the template. The selectivity experiment revealed that the UCNP@MIFP has a broad-spectrum molecular recognition capability. Good linear relationships were obtained over the concentration range of 1–10 ng/mL for sulfamerazine, sulfamethazine, sulfathiazole, and sulfafurazole, with low limits of detection in the range of 1.37–2.35 ng/mL. The prepared UCNP@MIFP has the potential to detect four sulfonamide residues in food and environmental water.
A Highly Efficient Workflow for Detecting and Identifying Sequence Variants in Therapeutic Proteins with a High Resolution LC-MS/MS Method
Authors: Cadang Lance , Tam Chi Yan Janet , Moore Benjamin Nathan , Fichtl Juergen , Yang Feng
Published: April 2023

Large molecule protein therapeutics have steadily grown and now represent a significant portion of the overall pharmaceutical market. These complex therapies are commonly manufactured using cell culture technology. Sequence variants (SVs) are undesired minor variants that may arise from the cell culture biomanufacturing process that can potentially affect the safety and efficacy of a protein therapeutic. SVs have unintended amino acid substitutions and can come from genetic mutations or translation errors. These SVs can either be detected using genetic screening methods or by mass spectrometry (MS). Recent advances in Next-generation Sequencing (NGS) technology have made genetic testing cheaper, faster, and more convenient compared to time-consuming low-resolution tandem MS and Mascot Error Tolerant Search (ETS)-based workflows which often require ~6 to 8 weeks data turnaround time. However, NGS still cannot detect non-genetic derived SVs while MS analysis can do both. Here, we report a highly efficient Sequence Variant Analysis (SVA) workflow using high-resolution MS and tandem mass spectrometry combined with improved software to greatly reduce the time and resource cost associated with MS SVA workflows. Method development was performed to optimize the high-resolution tandem MS and software score cutoff for both SV identification and quantitation. We discovered that a feature of the Fusion Lumos caused significant relative under-quantitation of low-level peptides and turned it off. A comparison of common Orbitrap platforms showed that similar quantitation values were obtained on a spiked-in sample. With this new workflow, the amount of false positive SVs was decreased by up to 93%, and SVA turnaround time by LC-MS/MS was shortened to 2 weeks, comparable to NGS analysis speed and making LC-MS/MS the top choice for SVA workflow.
Chaos in QCD? Gap Equations and Their Fractal Properties
Authors: Klähn Thomas , Loveridge Lee C. , Cierniak Mateusz
Published: April 2023

In this study, we discuss how iterative solutions of QCD-inspired gap-equations at the finite chemical potential demonstrate domains of chaotic behavior as well as non-chaotic domains, which represent one or the other of the only two—usually distinct—positive mass gap solutions with broken or restored chiral symmetry, respectively. In the iterative approach, gap solutions exist which exhibit restored chiral symmetry beyond a certain dynamical cut-off energy. A chirally broken, non-chaotic domain with no emergent mass poles and hence with no quasi-particle excitations exists below this energy cut-off. The transition domain between these two energy-separated domains is chaotic. As a result, the dispersion relation is that of quarks with restored chiral symmetry, cut at a dynamical energy scale, and determined by fractal structures. We argue that the chaotic origin of the infrared cut-off could hint at a chaotic nature of confinement and the deconfinement phase transition.
Interband Electron Transitions Energy in Multiple HgCdTe Quantum Wells at Room Temperature
Authors: Mikhailov Nikolay N. , Dvoretsky Sergey A. , Remesnik Vladimir G. , Uzhakov Ivan N. , Shvets Vasyliy A. , Aleshkin Vladimir Ya.
Published: April 2023

The studies of the interband electron transition energy in multiple Hg1-xCdxTe/Hg1-yCdyTe quantum wells (MQWs) at room temperature were carried out. The MQWs were grown on the (013) GaAs substrate by molecular beam epitaxy, with the layer composition and thickness being measured by the in-situ ellipsometric parameters measurement at the nanometer level. The Hg1-xCdxTe barrier composition and width were x = 0.69 and 30 nm, respectively. The Hg1-yCdyTe well composition was y = 0.06–0.10, and the width varied in the range of 2.7–13 nm. The experimental data of the interband electron transition energy were determined by the absorption spectral analysis. The calculation of the interband electron transition energy was carried out on the basis of the four-band Kane model. A good agreement between the experimental and calculated data was obtained. It was shown that MQWs may be used as a photosensitive material for creating infrared optoelectronic devices operating in different modes in the range of 3–10 μm at room temperature.
Broadband Microwave Photonic Mixer with Flexibly Tunable Phase Shift and Supporting Dispersion-Induced Power-Fading-Free Fiber Transmission
Authors: Yuan Mingxiu , Peng Di , Qin Yuwen , Li Jianping , Xiang Meng , Xu Ou , Fu Songnian
Published: April 2023

A broadband microwave photonic mixer with tunable phase shift and supporting dispersion-induced power-fading-free fiber transmission is proposed and demonstrated based on a dual-polarization dual-parallel Mach–Zehnder modulator (DP-DPMZM). In this scheme, the intermediate-frequency (IF)/radiofrequency (RF) signal and the local oscillation (LO) signal are applied to the four sub-MZMs biased at their minimum transmission points via a power splitter and a 90° hybrid coupler, respectively. Through mutual beating between the IF/RF and the LO modulation sidebands in a high-speed photodetector at the remote site, high-efficiency frequency conversion is achieved. The dispersion-induced power fading over long-distance fiber transmission is eliminated through setting the biased-induced phase difference between the parent-MZMs in the two sub-DPMZMs of the DP-DPMZM to be π/2. In addition, the phase shift of the frequency-converted signal can be continuously tuned over 360° through synchronously adjusting the bias voltages of the parent-MZMs in the two sub-DPMZMs. The proposed scheme is experimentally demonstrated, where a microwave photonic mixer with a 6-dB operation bandwidth of 40 GHz and supporting dispersion-induced power-fading-free transmission over 20 km SMF is realized. Meanwhile, a continuously tunable phase shift over 360° in the frequency range of 0.1 GHz to 29.9 GHz is achieved, where the power variation during phase tuning is smaller than 4 dB.
Automatic Inhomogeneous Background Correction for Spatial Target Detection Image Based on Partition Processing
Authors: Jiang Chun , Chen Tao , Lu Changzheng , Wu Zhiyong , Liu Changhua , Shao Meng , Cao Jingtai
Published: April 2023

High-resolution imaging with wide field of view (FoV) ground-based telescopes is often affected by skylight background and noise due to the detector, resulting in an inhomogeneous background. In this paper, we propose an improved method for spatial image non-uniformity correction based on partition processing. First, an evaluation metric is introduced to evaluate the partition size and automatically iterate a suitable partition value for different scenarios based on the different operating conditions of the telescope. Then, we iteratively calculate the mean and variance in each partitioned region to extract the background of each partitioned region. Finally, after applying bilinear interpolation to the background extracted from each region, the inhomogeneous background is obtained and removed from the original image. The experiments on the simulated and real images show that the proposed method can effectively remove the inhomogeneous background of spatial images and meet the requirements of the real-time processing of high-resolution images under long exposure conditions.
Chemical Characterization and Quality Assessment of Copaiba Oil-Resin Using GC/MS and SFC/MS
Authors: Lee Joseph , Wang Mei , Zhao Jianping , Ali Zulfiqar , Hawwal Mohammed F. , Khan Ikhlas A.
Published: April 2023

In recent years, the popularity of copaiba oil-resin has increased worldwide due to its medicinal value and wide applications in industry. Despite its popularity, the oil has not been standardized by industry or regulatory agencies. Product adulteration in order to maximize profits has become a problem. To address these issues, the current study describes the chemical and chemometric characterization of forty copaiba oil-resin samples by GC/MS. The results demonstrated, with the exception of commercial samples, that all sample groups contained six characteristic compounds (β-caryophyllene, α-copaene, trans-α-bergamotene, α-humulene, γ-muurolene, and β-bisabolene) in varying concentrations. Furthermore, compositional patterns were observed in individual groups which corresponded to sample origin. Within the commercial group, two samples did not contain or contained only one of the characteristic compounds. Principal component analysis (PCA) revealed distinct groups which largely corresponded to sample origin. Moreover, commercial samples were detected by PCA as outliers, and formed a group far removed from the other samples. These samples were further subjected to analysis using a SFC/MS method. Product adulteration with soybean oil was clearly detected, with each individual triglyceride in soybean oil being unambiguously identified. By combining these analytical techniques, the overall quality of copaiba oil-resin can be assessed.
Disturbing Effect of Intra-Tissue Temperature Sensors in Pre-Clinical Experimental Studies of Radiofrequency Cardiac Ablation: A Computer-Based Modeling Study
Authors: Cuenca-Dacal Luis , Berjano Enrique , González-Suárez Ana , Pérez Juan J.
Published: April 2023

Background: Preclinical studies on radiofrequency (RF) cardiac ablation (RFCA) use very small temperature sensors in specific positions in the tissue subjected to RF heating. Despite the sensors’ small size, the proximity to the ablation electrode and the extremely high thermal gradient around the electrode means that the presence of the temperature sensors could distort the temperatures recorded. Our objective was to assess the thermal impact of intra-tissue temperature sensors during RFCA. Methods: 3D RFCA models were built including different temperature sensors based on fiber optics and T-type thermocouples. Constant power ablation was simulated for 10 s. Results: The results showed that the disturbance caused by the presence of the T-type thermocouples was considerably greater (one order of magnitude) than that caused by the optical fibers. The closer the sensor was to the ablation electrode, the greater the greater the disturbance was and the more it increased with time in sensors more than 3 mm deep. The fiber optic measurements always slightly underestimated (<0.2 °C) the tissue temperature that would exist without the sensors, while the disturbance caused by the T-type thermocouples did not always result in underestimation but depended on the depth of the sensors parallel to the catheter. Conclusions: The presence of thermocouples inserted into the tissue close to the RF ablation electrode involves a disturbance that could affect the measured temperature value, although it does not substantially alter the shape and size of the thermal lesion. Optical fibers cause much less disturbance, possibly due to the absence of internal metal parts that favor heat conduction.
Shape Memory Graphene Nanocomposites—Fundamentals, Properties, and Significance
Authors: Kausar Ayesha , Ahmad Ishaq , Aldaghri O. , Ibnaouf Khalid H. , Eisa M. H.
Published: April 2023

Shape memory nanocomposites are excellent smart materials which can switch between a variable temporary shape and their original shape upon exposure to external stimuli such as heat, light, electricity, magnetic fields, moisture, chemicals, pH, etc. Numerous nanofillers have been introduced in shape memory polymers such as carbon nanotubes, graphene, nanodiamonds, carbon nanofibers, etc. Among nanocarbons, graphene has attracted research interest for the development of shape memory polymer/graphene nanocomposites. Graphene is a unique one-atom-thick two-dimensional nanosheet of sp2-hybridized carbon atoms. Graphene has been used as an effective nanofiller in shape memory polymeric nanocomposites owing to its remarkable electrical conductivity, flexibility, strength, and heat stability. Thermoplastics as well as thermoset matrices have been used to form the shape memory nanomaterials with graphene nanofiller. In shape memory polymer/graphene nanocomposites, their shape has been fixed above the transition temperature and then transformed to the original shape through an external stimulus. The inclusion of graphene in nanocomposites can cause fast switching of their temporary shape to their original shape. Fine graphene dispersion, matrix–nanofiller interactions, and compatible interface development can lead to high-performance shape memory graphene-derived nanocomposites. Consequently, this review focuses on an important class of shape memory graphene-based nanocomposites. The fabrication, physical properties, and shape memory actuation of polymer/graphene nanocomposites are discussed. The stimuli-responsive polymer/graphene nanocomposites mostly revealed heat-, electricity-, and light-induced effects. The inclusion of graphene enhanced the physical/covalent linking, shape recovery, shape fixity, flexibility, and crystallization effects in the polymers. Furthermore, potential applications of these materials are observed in the aerospace/automobile industries, civil engineering, and biomaterials.
Special Issue on “Advanced Combustion and Combustion Diagnostic Techniques”
Authors: Wang Zhihua
Published: April 2023

Our world still greatly relies on the combustion process to convert fuel into power and heat for purposes such as gas turbines, internal combustion (IC) engines, jet engines, rockets, boilers, and furnaces [...]
Fig Leaves (Ficus carica L.): Source of Bioactive Ingredients for Industrial Valorization
Authors: Shiraishi Carlos S. H. , Zbiss Yosra , Roriz Custódio Lobo , Dias Maria Inês , Prieto Miguel A. , Calhelha Ricardo C. , Alves Maria José , Heleno Sandrina A. , V. da Cunha Mendes , Carocho Márcio , Abreu Rui M. V. , Barros Lillian
Published: April 2023

The fig tree (Ficus carica L.) was one of the first domesticated trees. In 2019, the world’s fig fruit production was estimated at 1153 tons. However, fig leaves are not utilized, resulting in copious quantities of bio-waste. To identify promising fig tree varieties, hydroethanolic extracts were prepared from the leaves of five fig tree varieties (Pasteliere—PA, Longue d’Aout—LA, Dauphinie—DA, Boujassote Noire—BN, and Marseille—MA). The variety with the highest concentration of organic acids was BN (146.5 mg/g dw), while glucose, fructose, and sucrose were the predominant sugars across all varieties. All extracts present α-tocopherol as the prevalent tocopherol isoform (above 78%), while PUFA fatty acids were predominant, ranging from 53% to 71% of total fatty acids. BN showed moderate antioxidant activity (EC50 0.23 ± 0.01 mg/mL), while the DA variety presented promising cytotoxicity against the tumor AGS and MCF-7 cell line (GI50 158 ± 13 and 223 ± 21 μg/mL) and especially in the inhibition of Nitric Oxide Production evaluation (IC50 20 ± 5 μg/mL). The DA activities are probably related to high concentrations of flavonoids, specifically the predominant apigenin-C-hexoside-C-pentoside and quercetin-O-deoxyhexosyl-hexoside. Finally, the BN and DA varieties showed good antimicrobial activity, especially against Yersinia enterocolitica. Fig leaves can be considered sustainable sources of industrially valuable bioactive molecules, and several potential applications were highlighted.