bluuebunny/crossref_metadata_2025 huggingface dataset:
Sepsis Prediction in Hospitalized Children: Model Development and Validation
Authors: Stephen Rebecca J. , Carroll Michael S. , Hoge Jeremy , Maciorowski Kimberly , Jones Roderick C. , Lucey Kate , O’Connell Megan , Schwab Carly , Rojas Jillian , Sanchez-Pinto L. Nelson
Published: August 2023

BACKGROUND AND OBJECTIVES

Early recognition and treatment of pediatric sepsis remain mainstay approaches to improve outcomes. Although most children with sepsis are diagnosed in the emergency department, some are admitted with unrecognized sepsis or develop sepsis while hospitalized. Our objective was to develop and validate a prediction model of pediatric sepsis to improve recognition in the inpatient setting.

METHODS

Patients with sepsis were identified using intention-to-treat criteria. Encounters from 2012 to 2018 were used as a derivation to train a prediction model using variables from an existing model. A 2-tier threshold was determined using a precision-recall curve: an “Alert” tier with high positive predictive value to prompt bedside evaluation and an “Aware” tier with high sensitivity to increase situational awareness. The model was prospectively validated in the electronic health record in silent mode during 2019.

RESULTS

A total of 55 980 encounters and 793 (1.4%) episodes of sepsis were used for derivation and prospective validation. The final model consisted of 13 variables with an area under the curve of 0.96 (95% confidence interval 0.95–0.97) in the validation set. The Aware tier had 100% sensitivity and the Alert tier had a positive predictive value of 14% (number needed to alert of 7) in the validation set.

CONCLUSIONS

We derived and prospectively validated a 2-tiered prediction model of inpatient pediatric sepsis designed to have a high sensitivity Aware threshold to enable situational awareness and a low number needed to Alert threshold to minimize false alerts. Our model was embedded in our electronic health record and implemented as clinical decision support, which is presented in a companion article.
Ressignificando o grito da independência por meio da literatura infantil
Authors: Lopez Cristian Javier , Fant Carla Cristiane Saldanha , Scopel da Silva Rosângela Margarete
Published: August 2023

O objetivo deste artigo é apresentarmos um estudo comparativo das relações estabelecidas entre o quadro Independência ou Morte (1888), de Pedro Américo e a narrativa híbrida de história e ficção infantil Independência ou Morte! (2006), de Juliana de Faria. Na diegese analisada, destaca-se como uma das personagens o próprio Pedro Américo, quem explica detalhes de sua obra pictórica que não correspondem ao discurso oficial. Nesse sentido, o estudo das relações entre o discurso historiográfico, a literatura infantil e a pintura possibilitam que o jovem leitor tenha acesso, desde os anos iniciais, a análises que visam à ampliação de conhecimentos, principalmente, sobre o uso da linguagem. Dessa maneira, podemos corroborar o potencial do texto literário, especialmente o híbrido de história e ficção infantil, na formação de um leitor literário já desde os anos iniciais do Ensino Fundamental. Ancoramos nossas reflexões nos pressupostos de Lima (2006), Palermo (2013), Samoyault (2008), Mendoza Fillola (1994), Fleck (2017), entre outros. Consideramos que as narrativas híbridas de história e ficção brasileiras produzidas para leitores infantis podem revisitar o passado, localizando elementos ou espaços que nos dão a possibilidade de ressignificar a história tradicional, além de colaborar, de forma concreta, na ampliação de conhecimentos.
Exploring the relationship between mental health, drug use, personality, and attitudes towards psilocybin-assisted therapy
Authors: Ross Benjamin M. , Neill James T.
Published: August 2023

Background

Psilocybin, the psychoactive compound in magic mushrooms, is increasingly discussed in terms of its psychotherapeutic potential; however, little is known about community attitudes towards psilocybin assisted therapy (PAT).

Aims

To address the question: What are the public's attitudes towards psilocybin and psilocybin-assisted therapy? And what factors explain these attitudes?

Methods

This study investigated the attitudes of 118 young adults in the Australian Capital Territory through an online survey.

Results

Participants who were more open to experience and who had used recreational drugs were more likely to have positive attitudes towards all aspects of PAT. Additionally, psychedelic drug use and agreeableness was positively associated with attitudes towards psilocybin safety, legality, and research; and psilocybin use was positively associated with attitudes towards psilocybin knowledge and acceptability.

Conclusions

This convenience sample of young adults was generally positively disposed towards PAT. People who were more open to experience and who had used recreational or psychedelic drugs had more favourable attitudes towards PAT.
A pszichiátriai betegségekre jelentős korai halálozási arányszámok jellemzők: szomatikus komorbiditás és mortalitás autizmusspektrum-zavarban és szkizofréniában
Authors: Bitter István , Czobor Pál , Kakuszi Brigitta , Réthelyi János
Published: August 2023

A pszichiátriai betegségekkel összefüggő jelentős halálozásért nagy részben a
szomatikus betegségekkel való komorbiditás esetén bekövetkező korai halálozás,
továbbá részben az öngyilkosság és a balesetek a felelősek. Irodalmi
összefoglalónkban bemutatjuk, hogy az autizmusspektrum-zavarral és a
szkizofréniával diagnosztizált személyek halálozási kockázata több mint 2-szeres
a kontrollcsoportokhoz képest. A szkizofrénia diagnózisát illetően hazai adatok
is rendelkezésre állnak, amelyek az emelkedett halálozási kockázat tekintetében
megegyeznek a nemzetközi adatokkal. Magyarországon kb. 2,4-szeres a szkizofrénia
diagnózisával élő személyek halálozási rizikója a korban, nemben és irányítószám
szerint illesztett kontrollcsoporthoz képest. Különösen emelkedett a halálozás
kockázata szkizofréniában a fiatalok között (7–10-szeres), és kiemeljük, hogy
bár a férfiak halálozási rizikója nagyobb, mint a nőké, a szkizofréniával
diagnosztizált nők halálozási kockázata a női kontrollcsoporthoz képest (relatív
kockázat) nagyobb, mint a férfiaké. Figyelembe véve a pszichiátriai betegségek
magas prevalenciáját, a szomatikus betegségek esetében regisztrált hazai
halálozási mutatók javításának fontos feltétele a szomatikus betegségek ellátása
során a komorbid pszichiátriai betegségek korai diagnózisa és kezelése. Az itt
ismertetett adatok segíthetik a halálozás csökkentéséhez szükséges reformokat az
orvosképzésben és a szakképzésben, valamint az egészségügyi ellátás
szervezésében. Orv Hetil. 2023; 164(33): 1287–1293.
Ganoderma lucidum extract reverses hepatocellular carcinoma multidrug resistance via inhibiting the function of P-glycoprotein in vitro and in vivo
Authors: Li Jing , Cao Lin , Yuan Cheng , Jiang Zhaojian , Cai Hongfei , Xu Wendong , Han Yaming , Chen Liang , Zhang Qin , Jiang Renwang , Liu Juyan
Published: August 2023

Cancer is a leading cause of death globally. Chemotherapy still plays an indispensable role in the clinical treatment of cancer. However, the emergence of multidrug resistance (MDR) has greatly obstructed the further application of chemotherapy agents. Ganoderma lucidum (G. lucidum) is a traditional Chinese medicine as well as an edible mushroom. In this study, we first explored the effect of six extract samples derived from G. lucidum on the cell viability of adriamycin-resistant human hepatocellular carcinoma multidrug-resistant cell subline (HepG-2/ADM). All these samples showed no obvious toxicity to cells; however, only G. lucidum ethanol extract could reverse resistance to doxorubicin and paclitaxel. Then the P-glycoprotein (P-gp) function in hepatocellular carcinoma G2 (HepG-2) and HepG-2/ADM cells was determined after incubated with these samples, and we found that G. lucidum ethanol extract could inhibit P-gp function in vitro. Furthermore, G. lucidum ethanol extract could reverse resistance to paclitaxel in HepG-2/ADM tumor-bearing mice in vivo, while the protein expression level of P-gp was unchanged. Taken together, these results indicated the potential role of G. lucidum ethanol extract in reversing MDR in the clinical treatment of hepatocellular carcinoma.
O Surgimento Do Profetismo Como Resposta Às Crises No Ambiente Da Palestina Do 1º Século
Authors: Stephen Daniel Neves , Carneiro Marcelo Da Silva
Published: August 2023

A sociedade hodierna vem sofrendo diversas crises em seu contexto social, econômico, político e religioso. Este cenário não é tão diferente da situação em que Jesus e seus discípulos vivenciaram na Palestina do 1º século da era atual. Muitos movimentos surgiram como proposta para a resolução dos problemas e conflitos na sociedade daquele tempo, inclusive com o emprego da violência. Na contramão desta atitude, o movimento de Jesus, inspirado no profetismo antigo, surgiu com o objetivo de pacificar e ser uma resposta frente aos dramas vivenciados pelo povo. Portanto, que relevância o movimento profético de Jesus teve para a Palestina do 1º século? Este estudo procurará responder esta questão e destacar a importância que os seguidores de Jesus têm na continuidade desta missão outorgada pelo seu líder maior, Jesus de Nazaré.
A celebração da Quaresma
Authors: Siqueira Tércio Machado
Published: August 2023

A palavra “quaresma” não é citada no texto bíblico, mas os membros do Concílio de Niceia foram bons intérpretes da Bíblia ao incluir na liturgia da Igreja Cristã essa celebração. A quaresma não é uma doutrina, mas ela tem sua base na história do êxodo: ela sinaliza, para o povo cristão, que os problemas e as dificuldades não são definitivos. A Páscoa interrompe esse período de escassez, trazendo alegria e paz para o povo.
РОЗРОБКА РЕЦЕПТУР НАПОЇВ РОСЛИННИХ НА ОСНОВІ СОНЯШНИКОВОГО БІЛКОВОГО КОНЦЕНТРАТУ
Authors: TKACHENKO N. , CHAHAROVSKYI O. , DIDUKH E.
Published: August 2023

В роботі зазначено фактори, які сприяють розвитку галузі виробництва «рослинного молока» в Україні, наведено аналіз ринку «рослинного молока» в Україні та світі, показано перспективи розвитку даного сегменту протягом найближчого десятиліття у країні, окреслено сегмент українців, які є потенційними споживачами цільового продукту – «рослинного молока». На основі аналізу літературних даних обґрунтовано актуальність та доцільність перероблення концентратів білків соняшника, які сьогодні в Україні виробляють підприємства олійно-жирової галузі, у напої рослинні. Показано перспективність та доцільність застосування наповнювачів – какао-порошку, цукру, натуральних ароматизаторів та барвників, амілолітичних ферментів, згущувачів, спецій (куркума, карі, какао-порошок), регуляторів кислотності – у рецептурах рослинних напоїв на основі концентрату білкового сухого знежиреного соняшникового. Базові рецептури рослинних напоїв були складені на основі аналізу літературних даних щодо хімічного складу молока коров’ячого питного та різних видів існуючого в Україні «рослинного молока» із застосуванням у якості основної сировини концентрату білків соняшника сірого кольору, який виробляє у промислових масштабах український олійно-екстракційний завод №1 ТОВ «Потоки», м. Дніпро. На основі аналізу органолептичних показників рослинних напоїв, вироблених за базовими рецептурами, було обґрунтовано доцільність виробництва іншого виду основної сировини – концентрату білкового сухого знежиреного соняшникового – з покращеними органолептичними показниками, зокрема, з білим кольором. Базове підприємство удосконалило технологію виробництва білкового соняшникового концентрату і лінійка напоїв рослинних була розроблена на основі концентрату білкового сухого знежиреного соняшникового білого кольору. Результатом роботи стали три рецептури напоїв рослинних: напій із ароматизатором «Вершковий», напій із ароматизатором «Диня» та напій «Шоколадний». Проведена органолептична оцінка вироблених за розробленими рецептурами напоїв рослинних засвідчила їх високі сенсорні характеристики.
Social Farms as Social Enterprises in the Czech Republic
Authors: Hudcová Eliška
Published: August 2023

Background: Social entrepreneurship has grown in popularity in the Czech Republic over the last few decades as a solution to the demand for long-term, socially responsible drivers of economic growth. As part of social entrepreneurship, social farming combines social services and the opportunity for work integration in a farm setting. This article employs EMES methodology to the study of social farming in the Czech Republic.Research objectives: Drawing on the findings of 15 case studies, the author analyses social farming in the context of the EMES model with a focus on the organisation and principal goals of Czech social farms.Research design and methods: The theoretical part is based on a review of the literature on social economy, social entrepreneurship, and social farming. The case study report that follows employs mixed data sets such as interviews, field notes, information gathered from publicly available sources, brochures, organisation laws, annual reports, newspaper articles and other media reports, as well as data from a variety of registers. Results: From a normative standpoint, social farming and social farms in the Czech Republic are comparable to those in Europe, but they are fewer in number and are subject to distinct regulation. Institutionally speaking, only social farms registered as institutes (zapsaný ústav) meet the EMES social enterprise criteria. Czech social farms are known for their emphasis on craftsmanship, tradition, volunteerism, strong bonds with a particular location, ecology, links with direct suppliers, solidarity, and non-monetary trade.
Analysis of Brain Thermometric Data Obtained by Microwave Radiothermometry
Authors:   Volgograd State University, Popov Illarion , Krylova Aleksandra ,   Volgograd State University
Published: August 2023

This paper discusses the effectiveness of using the method of microwave radiothermometry in examinations of brain diseases, namely the state of disordered consciousness. In contrast to most methods of examinations by this method, the measurements of the brain were carried out in only 2 different frontal areas for 2 days with a frequency of 4 hours. Therefore, the aim of the study was to identify the effectiveness of a diagnostic model based on the dynamics of temperature changes. The work showed that in healthy patients there is a circadian rhythm: during the day the temperature rises, at night it decreases. At the same time, such dynamics is not observed in patients with disordered consciousness. Based on this knowledge, a conceptual and mathematical model were proposed. The first of them describes the characteristic features of healthy and sick patients. The second one quantifies these features. The constructed mathematical model was tested in the classification problem. The Naive Bayes classifier was used as a classifier. As a result of computational experiments, it was shown that for 500 iterations the classifier made a mistake on only 1 sick patient and 5 healthy ones. Thus, the effectiveness of the method of microwave radiothermometry in the task of examining patients with disordered consciousness was shown.
NP-Completeness of the Problem of Constructing a Graph with a Minimum Stretch Factor
Authors:   Volgograd State University, Khizhnyakova Ekaterina
Published: August 2023

In this paper, we consider the problem of constructing a planar connected weighted graph on a given set of points in such a way that the sum of the shortest paths between all pairs of vertices is the minimum possible. In other words, the problem of constructing a graph with a minimum stretch factor. The euclidean distance between the ends is taken as the weight of the edge. It is proved that such a problem is NP-complete. This problem arises when planning transport networks, since it is the stretch factor of the graph of the transport network that directly indicates the level of overrun of transport, which should be as small as possible in order to minimize the resources spent. But since this problem is NP-complete, a heuristic algorithm for solving the problem under consideration is proposed, which is based on sorting some simple paths and has a recursive character. This algorithm always builds a triangulation, since only in this class of graphs is it possible to solve the problem. A number of experiments have been carried out on the statistical study of the stretch factor of the triangulation constructed as a result of the operation of the above algorithm on randomly generated points. Experiments show that the losses are about 1.5–7.8% , and for real cities this parameter is about 30%.
Mathematical-Cartographic Modeling of Climate Dynamics in the Rostov Region using Global Meteorological Data
Authors:   Federal Scientific Centre of Agroecology of the Russian Academy of Sciences, Matveev Stefan
Published: August 2023

The scientific work presents mathematical and cartographic modeling of the climate dynamics of the Rostov region using the global meteorological product CRU TS version 4.06 in the period from 2020 to 2040. The work used CRU TS global climate data from 1960 to 2020 to model data for 2030 and 2040. A technique for mathematical-cartographic modeling of climate dynamics based on global climate products is presented. Using the geographic information system QGIS version 3.18, raster images were vectorized, which made it possible to export them to a MS Excel spreadsheet. Based on the data obtained, 2 tables were prepared. One includes actual data for 2020 and modeling data in terms of the average annual air temperature for 2030 and 2040 for the municipal districts of the Rostov region. The second table includes actual data for 2020 and simulation data in terms of the average annual air temperature for 2030 and 2040 for the urban districts of the Rostov region. The relationship between actual data for 2020 and data for 2030 and 2040 does not exceed 10%. This allows us to say that a stable climate has been established on the territory of the Rostov region, which is not subject to serious climatic changes in the future. According to the actual data and simulation data, maps were created and compiled using the interpolation method of inversely weighted distances in terms of the average annual air temperature for 2020, 2030, and 2040.
Designing structure and E. coli strain-producer bearing SARS-CoV-2 N, S, M, E protein-related sequence antigen
Authors: Kopat Vladimir V. , Riabchenkova Anastasia A. , Chirak Evgenii L. , Chirak Elizaveta R. , Saenko Anna I. , Kolmakov Nikolai N. , Simbirtsev Andrey S. , Dukhovlinov Ilya V. , Totolian Areg A.
Published: August 2023

T-cell immune response is extremely important in protecting human body from diverse viral infections. It is known that it can ensure viral clearance and complete recovery in patients with humoral immunodeficiency. COVID-19 patients were found to have T-cell response primarily directed against SARS-CoV-2 structural S, M, N, E proteins, with nucleocapsid protein being most conserved. To assess patients immunity against coronavirus infection and evaluate an effectiveness of vaccine candidates, it is necessary to develop an optimal diagnostic antigen to evaluate arising T-cell response against SARS-CoV-2 antigenic determinants. A diagnostic test to determine host specific susceptibility to SARS-CoV-2 infection should target conserved regions of global SARS-CoV-2 variants. The study was aimed to develop a structure of an antigen bearing conserved and immunogenic sequences derived from SARS-CoV-2 structural proteins and to obtain an Escherichia coli producer strain containing a recombinant protein to be subsequently used for assessing antiviral T-cell immunity. Developing of the antigen was performed in silico: TepiTool and NetMHCIIpan were used to predict and identify high affinity epitopes spanning SARS-CoV-2 E, M, N, S proteins and MHC II binding. Several variants of recombinant antigen proteins were constructed, from which one was selected based on its physicochemical properties: isoelectric point, hydrophobicity index and aliphatic index, as well as 3D representation built by using the I-TASSER. The sequence was synthesized and cloned into the pET24a(+) vector. The resulting plasmid pCorD_PS was transformed into E. coli DH5 followed by Rosetta (DE3). The strain-producer of the recombinant E. coli protein CorD_PS was assessed for the presence and stability of IPTG-induced antigen protein expression and elimination of recombinant coronavirus antigen-bearing plasmid. Based on the study data, an antigen was developed consisting of conserved regions from SARS-CoV-2 S, M, N, E proteins. A 53 kDa recombinant protein was predicted to be stable in aqueous solutions with isoelectric point of 9.56 potentially allowing to simplify protein purification from E. coli cells. Plasmid DNA pCorD_PS (6695 bp) encoding final recombinant coronavirus antigen cloned into pET24a(+) vector was obtained. A stable, productive E. coli CorD_PS strain was obtained. The obtained strain-producer resulting in recombinant E. coli CorD_PS antigen is stable allowing to move on to design antigen purification technique and further develop SARS-CoV-2-specific diagnostic test system.
Features of NK cell phenotype virus genotype-driven chronic viral hepatitis C
Authors: Savchenko Andrei A. , Tikhonova Elena P. , Anisimova Anna A. , Kudryavtsev Igor V. , Belenjuk Vasilij D. , Borisov Alexandr G.
Published: August 2023

Elimination of the hepatitis C virus (HCV) due to direct antiviral drug (DAD) action affects alteration in virus phenotype and, accordingly, NK cell functional activity. However, the published data are very contradictory. The aim of the study was to investigae alterations in NK cell subset phenotype after DAD treatment of HCV genotype-dependent chronic viral hepatitis C (CVHC) patients. Materials and methods. 111 CVHC patients and 21 healthy volunteers were examined. The diagnosis was established on epidemiological, clinical and laboratory data. All 111 subjects with CVHC received direct antiviral drugs Sofosbuvir and Velpatasvir for 12 weeks. The study of the NK cell phenotypes wwas analyzed by multicolor flow cytometry. Results. A decreased count of cytokine-producing along with increased frequency of cytotoxic NK cells were found in CVHC patients blood samples with various HCV genotypes prior to DAD treatment. The imbalance of cytotoxic cells with a high level of functional activity was also found in CVHC patients regardless of HCV genotype. The patients with HCV genotypes 1 and 3 showed significantly increased level of immunoregulatory NK cells. In addition, increased count of glycohydrolase (CD38) and ecto-5'-nucleotidase (CD73)-expressing NK cells were found in patients with HCV genotypes 1 and 3. Hence, such alterations in NK cell phenotype in CVHC patients were presented as sustained high viral load which peaking at carriers of HCV genotype 1 that was minimal in patients with HCV genotype 2. The most prominent change in NK cells after DAD treatment was found in CVHC patients with HCV genotype 2 (normalization of CD8-expressing NK cell subset composition and count). Only patients with HCV genotype 2 after treatment had increased frequencies of peripheral blood double-negative CD38–CD73– NK cells. Patients with HCV genotypes 1 and 3 also showed minimally improved in NK cell subset composition after DAD treatment. Conclusion. Evaluation of specific changes in NK cell phenotype during DAD treatment of CVHC patients driven by HCV genotype undoubtedly is of importance and high relevance. The results obtained are novel and complement the insights into CVHC immunopathogenesis. Analysis of NK cell phenotypes and functional activity in patients with CVHC may promote development of new methods for treating HCV infection.
Optimization and validation of flow cytometry method for quantification of SARS-CoV-2 antigen-reactive human memory T cells
Authors: Strizhakova Olga M. , Pershin Andrey S. , Kazarov Alexander A. , Lyagoskin Ivan V. , Bahareva Yana A. , Vasil'ev Alexander P. , Nikonova Yulia A. , Egorova Irina Yu. , Shukurov Rahim R. , Khamitov Ravil A.
Published: August 2023

A proper and representative monitoring of SARS-CoV-2 herd immunity including a long-term health impact on recovered patients and vaccinated individuals is of great importance. For this, a monitoring campaign should assesses both humoral and T-cell immune arms. Upon that, analyzing antigen specific-cell activation and cellular phenotype are informative. We developed a flow cytometry method for detection of intracellular IFN-producing antigen-reactive T cells after exposure of human peripheral blood mononuclear cells (PBMC) to SARS-CoV-2 virus antigens. The method was validated according to the following characteristics: sensitivity, specificity, precision, and robustness. We used positive samples from donors recovered from COVID-19 and negative samples from donors who had no contact with COVID-19 patients and lacking antibodies to SARS-CoV-2. All samples were tested by laboratory methods. Peripheral blood mononuclear cells were isolated from donor blood by centrifugation in a Ficoll density gradient. Specific T cells were stimulated with S-protein as well as N, M, ORF3a, and ORF7a protein peptides to count IFN-producing T cells by flow cytometer. The data were statistically analyzed. The area limited by ROC-curve and false positive rate (AUC) for CD4 and CD8 cells was from 0.97 to 1.00. Precision was considered acceptable because the coefficient of variation for all PBCM did not exceed 20%. Robustness was confirmed for frozen and freshly prepared PBMC samples. The thresholds levels to recognize immune and non-immune samples were defined for CD4-positive T-cells (0.029%) and CD8-positive T-cells (0.0640.068%). Also, acceptance criteria for positive and negative controls were defined. Based on the validation, the suitability of the method Evaluation of antigen-reactive T cells that produce intracellular IFN in response to SARS-CoV-2 virus antigens by flow cytometry was confirmed. The method allows for reliable data that was used to characterize standard control samples for internal quality control of TigraTest SARS-CoV-2 kits.
Pages of the common history of Kazan and Saint Petersburg microbiology schools
Authors: Isaeva Guzel S. , Sboychakov Victor B. , Gumilevsky Boris Yu.
Published: August 2023

The paper is prepared for the 100th anniversary of the Department of Microbiology at the Military Medical Academy and the 100th anniversary of naming Louis Pasteur Saint Petersburg Research Institute of Epidemiology and Microbiology. The article is devoted to the history of interaction between Kazan and Saint Petersburg (Leningrad) microbiological schools from early XXth century to the present day. It provides historical milestones in developing Departments of Microbiology at the Kazan State Medical University and the Military Medical Academy as well as research departments both in the Kazan Research Institute of Epidemiology and Microbiology and the Saint Petersburg Research Institute of Epidemiology and Microbiology, highlights aspects of the joint establishment and development of the Kazan and Saint Petersburg Microbiology Schools, provides brief biographical data of the microbiology department staff members, whose activities were related to work in such institutions. Biographical information, personal characteristics, major directions in scientific and practical activities of famous microbiologists who worked in various periods of own lives in Saint Petersburg (Leningrad) and Kazan (V.M. Aristovsky, R.R. Geltzer, P.N. Kashkin, I.I. Pryakhin, A.R. Konova, A.G. Grigorieva-Berenstein) are presented. The efforts of Leningrad and Kazan scientists during the Great Patriotic War scientists to jointly develop and improve methods of industrial manufacture of antibiotics, therapeutic and prophylactic sera, bacteriophages are covered in detail. The article was prepared using primary sources: publications, memoirs of employees of the Department of Microbiology at the KSMU, VMA, KNIEM, Saint Petersburg Pasteur Institute, documents from the archives of personnel departments, as well as the National Archive of the Republic of Tatarstan, archival materials of the Departments of Microbiology of KSMU and VMA. The main scientific achievements of representatives of the Kazan and Saint Petersburg Microbiology Schools, their scientific contribution to development of microbiology field are described. Finally, it is noted about a need to preserve historical memory about the contribution of Russian scientists to development of microbiology field.
The 35th anniversary of the North-Western District center for AIDS prevention and control at the Saint Petersburg Pasteur Institute
Authors: Belyakov Nikolay A. , Ogurtsova Svetlana V. , Rassokhin Vadim V. , Lioznov Dmitry A. , Yastrebova Elena B. , Boeva Ekaterina V. , Totolyan Areg A.
Published: August 2023

The organization of the North-Western District Center for AIDS Prevention and Control (AIDS Center) originates from the onset of the HIV epidemic in the Russian Federation. Over the 35 years of work, the staff of the AIDS Center has made an invaluable contribution to the development of HIV diagnostics, the introduction of new clinical and preventive technologies, patient counseling, and training of qualified personnel. The anniversary article highlights the main stages regarding establishment of the AIDS Center as a structural unit of the Saint Petersburg Pasteur Research Institute. The versatile naturally multidisciplinary functions and tasks related to the AIDS Center organization, its major Russia-wide and global achievements are considered. Special attention is paid to the long-term international cooperation, the results of diverse implemented programs and projects supported by the World Health Organization (WHO) as well as the Joint United Nations Programme on HIV/AIDS. The AIDS Center continues to cooperate with the main scientific facilities such as the Pavlov First Saint Petersburg State Medical University, Institute of Experimental Medicine, N.P. Bekhtereva Institute of the Human Brain of the Russian Academy of Sciences, etc. The ongoing joint research invariably contributes to assessing the epidemiology and clinical course of various viral infections and comorbid conditions. The knowledge acquired becomes regularly summarized and published in a series of monographs, journal articles being presented demonstrated at conferences of various levels. Currently, the AIDS Center is represented by several structural divisions continuing to conduct practical and scientific activities on investigating HIV and viral hepatitis.
Modal Verbs in Sustainability Reports by the University of Cambridge and the University of Oxford
Authors: Kapranov Oleksandr
Published: August 2023

This article presents and discusses a study on modal verbs in sustainability reports that are communicated online by the University of Cambridge (UC) and the University of Oxford (UO) in the United Kingdom. The study is based upon a contention proposed by Fløttum (2010), who posits that modal verbs are used in sustainability discourse as micro-linguistic means that are involved in a range of pragma-communicative strategies. Following Fløttum (2010), the present study seeks i) to establish the frequency of the occurrence of the so-called “central” modal verbs, i.e. can/could, may/might, must, shall/should, and will/would and ii) to identify their involvement in the programa-communicative strategies in the sustainability reports by UC and UO. The sustainability reports by UC and UO were analysed in the computer program AntConc version 4.0.11 (Anthony, 2022) in order to compute the frequency of the central modal verbs. Thereafter, they were analysed qualitatively to establish their relationship with a range of pragma-communicative strategies that were employed in the sustainability reports by UC and UO, respectively. The quantitative computer-assisted analysis indicated that can and will were the most frequent modal verbs in the UC’s and UO’s sustainability reports. However, the qualitative investigation demonstrated that can and will were associated with different pragma-communicative strategies in the reports.  The findings were further discussed in the article.
SCS443 ISADORA: late ripening apple cultivar with very high fruit storage ability
Authors: Denardi Frederico , Kvitschal Marcus Vinicius , Argenta Luiz Carlos , Couto Marcelo , Araújo Leonardo
Published: August 2023

Abstract The objective of this study is to describe the agronomic attributes of the SCS443 Isadora apple tree cultivar and to highlight its potential use in Brazil. Obtained by the hybridization made at Epagri in 2001 between ‘Imperatriz’ and ‘Cripps Pink’, after 20 years of agronomic evaluations carried out in Caçador-SC and Fraiburgo-SC, ‘SCS443 Isadora’ was originated. This cultivar has a wide plasticity of adaptation to the southern Brazilian climate due to the medium chilling requirement to overcome dormancy. It has greater resistance to the main apple diseases than cv. Gala and fruit harvest is later than cv. Fuji. Its apples have a red skin and firm flesh (=18 lb), with high sugar content (= 14.5 %) and very crunchy and juicy texture, as well as high storage ability in post-harvesting. ‘SCS443 Isadora’ apples can be stored for 8 months, even in refrigerated air atmosphere chambers (21% O2) with inhibition of the ethylene response by the use of 1-methylcyclopropene (1-MCP). Even stored for long periods of time, the indices of quality loss, physiological disorders and fruit rotting in ‘SCS443 Isadora’ apples are lower than on Gala and Fuji apples.
Yield of essential oil from varieties of Citrus sinensis (L.) Osbeck
Authors: Santos Fernanda Rodrigues dos , Souza Jaqueline Lima da Conceição , Silva Barbara Rodrigues da , Pereira Caio César de Oliveira , Flores Rilner Alves , Souza Eli Regina Barboza de
Published: August 2023

Abstract Residues are generated after the processing of citrus fruits and can be used for essential oil extraction. Thus, the objective of this work was to evaluate the composition, yield, and quality of the proven oil of 30 orange varieties, as an alternative for the better use of its residues. The peels were crushed and subjected to hydrodistillation in a Clevenger-type. The samples were submitted to an Entirely Randomized Design (DIC) and the results were analyzed in Analysis of Variance, Principal Component Analysis, and Hierarchical Cluster Analysis. The greatest gains in essential oil were yielded by the varieties Valencia IAC (5.57%), Pera DP 06 (4.75%), Hamlin 20 (4.58%), and Pera DP 25 (4.28%). Valencia IAC has the potential to extract up to 7.24 kg of essential oil per ton of orange. Limonene represents 93.18 to 99.61% of the oil components, being the major compound among all varieties. Some varieties did not describe secondary compounds or more than one. These components are highly antioxidant and of great importance in the production of cosmetics, personal care products, homeopathy, help gastrointestinal diseases, among other uses. It is possible to conclude that the varieties have different compounds and oils, even when submitted to the same drying and oil extraction process, being the intrinsic characteristics of each variety.
Physico-chemical characterization of orange fruits on different rootstocks
Authors: Oliveira Vinicius de Souza , Zucoloto Moises , Ferreira Lusiane de Sousa , Barbosa Dimmy Herllen Silveira Gomes , Soares Walter dos Santos , Passos Orlando Sampaio
Published: August 2023

ABSTRACT The objective of this study was to evaluate the physico-chemical characteristics of fruits of 58 sweet orange tops cultivars on four rootstocks, namely: ‘Sunki Tropical’ mandarin and ‘Trifoliata’ Citrandarin ‘San Diego’, ‘Riverside’ and ‘Indio’. For this purpose, a completely randomized experimental design was used, in a factorial scheme (58 x 4). The characteristics analyzed were: equatorial diameter, fruit mass, peel thickness, number of seeds, soluble solids, titratable acidity, Ratio and juice yield. All the characteristics analyzed were influenced by both the tops cultivar and the rootstock, demonstrating that both factors interfere in the physicochemical quality of the fruits. The tops ‘Pera D-3’, ‘Pera Olimpia’, ‘Pera Bianchi’, ‘Jaffa’ and ‘Westin’ on all rootstocks evaluated showed characteristics in their fruits with soluble solids greater than 10 ºBrix, Ratio above 9.5 and juice yield greater than 45%, characteristics that define fruits with quality standards. Also, the tops ‘Pera Olimpia’, ‘Sincorá’ and ‘Westin’ on the rootstock ‘Sunki Tropical’, ‘Pera D-3’, ‘Pera D-25’, ‘Sincorá’, ‘Aquiri’ and ‘Russas P.S.’ on the rootstock ‘San Diego’, ‘Pera C-32’, ‘Pera D-3’, ‘Pera D-6’, ‘Pera E-6’, ‘Pera Bianchi’, ‘Pera CE-03 ‘, ‘Salustiana’ and ‘Westin’ on ‘Riverside’ rootstock are suitable for the juice production industry with Ratio between 14 and 16 and juice yield greater than 50%. It is also worth noting that the tops ‘Crescent’, ‘Natal Folha Murcha’, ‘Valencia Midknight’ and ‘Valencia Delta’ produced fruits without seeds on all rootstocks.
Essential oils from condiment and medicinal plants in the control of contaminants from the micropropa-gation of Myrciaria dubia
Authors: Barreto Hosana Carolina dos Santos , Chagas Edvan Alves , Melo Antônio Alves de , Etienne Bilovenie , Silva Deila Cristina Vieira da , Araújo Maria da Conceição da Rocha
Published: August 2023

Abstract The main limitation of the micropropagation of camu-camu (Myrciaria dubia) is related to in vitro contamination. In order to overcome contamination, the effect of essential oils was studied as an alternative to conventional chemical treatments. This study aimed to analyze the action of essential oils from four condiment and medicinal plants (Oregano, Origanum vulgare L.; Garlic, Allium sativum L.; Citronella, Cymbopogon nardus L.;and Ginger, Zingiber officinale Rosc.) in reducing microbial contamination growth and on the survival rate of explants in the micropropagation of camu-camu. The antimicrobial activity of essential oils was analyzed on the in vitro contamination of tissue culture from camu-camu at concentrations of 0.5, 1, 2, 3 and 4 µL mL-1 in woody plant medium (WPM), emulsified with 1% Polysorbate 80 (Tween 80). The antibacterial activity of essential oils on strains isolated from camu-camu tissue culture was also analyzed using agar diffusion and broth microdilution methods. The use of essential oils allowed a reduction in the rate of in vitro contamination in tissue culture, it being observed that, from the concentration of 2 µL mL-1, there was no manifestation of fungal contaminants, with a significant reduction in the rate of bacterial contamination, with the exception of ginger essential oil that showed significant contamination in all analyzed concentrations. Inversely in relation to the reduction in microbial growth in vitro, there is an increase in explant oxidation as concentrations increase, with citronella and oregano oils showing phytotoxic potential from the lowest concentrations. Garlic essential oil showed better balance, with lower oxidation rates and greater control of microorganisms in tissue culture at concentrations of 0.5 and 1 µL mL-1. Oregano and citronella essential oils showed better antibacterial activity in the agar diffusion test.
Steps of cryopreservation of coffee seeds: physiological responses and antioxidant systems
Authors: Souza Ana Cristina de , Costa Marina Chagas , Figueiredo Madeleine Alves de , Pereira Cristiane Carvalho , Coelho Stefânia Vilas Boas , Vilela Ana Luiza Oliveira , Pereira Diego de Souza , Rosa Sttela Dellyzete Veiga Franco da
Published: August 2023

ABSTRACT: The cryopreservation of plant germplasm at ultralow temperatures is an alternative technique for the long-term storage of seeds of the genus Coffea sp. However, for this technique to be successful, cell integrity must be maintained at all stages of the process on the basis of scientific research. The present study investigated validated cryopreservation protocols for Coffea arabica L. seeds and evaluate the effects on the physiological and biochemical characteristics of the seeds at each stage of the process. Seeds were dried on silica gel or with saturated saline solution, precooled or not in a biofreezer, immersed in nitrogen, and reheated in a water bath. After each of these steps, the physiological and biochemical quality of the seeds was determined. Pre-cooling is a step that can be dispensed with in the cryopreservation of Coffea arabica seeds, direct immersion in liquid nitrogen being more indicated. Coffea arabica L. seeds tolerate cryopreservation after rapid drying in silica gel up to water contents of 17 or 20% (wb), with greater survival at 17%. The enzyme activities of catalase, polyphenol oxidase and peroxidase are indicators of the quality of C. arabica L. seeds subjected to cryopreservation.
Vivencias sobre el cuidado en la vejez, análisis desde la gerontología crítica feminista
Authors: González Moreno Rosa Jazmín , Rangel Flores Yesica Yolanda
Published: August 2023

Resumen: El objetivo fue analizar las vivencias que sobre el cuidado tiene un grupo de hombres y mujeres en México, desde el enfoque de la gerontología crítica y feminista, apostando la existencia de experiencias diferenciadas que deberían ser consideradas en las políticas públicas orientadas al cuidado en la vejez. Se realizaron entrevistas en profundidad a diez personas, y mediante análisis de contenido se identificó que mientras ellas contemplan la experiencia desde una perspectiva del cuidado sostenido en la familia y la comunidad, ellos plantean la pertinencia de un cuidado sostenido en el mercado, aunque reconocen las limitaciones económicas para acceder a este. Ellas elaboran estrategias interpersonales y comunitarias para intercambiar los cuidados como bien, en tanto ellos resisten reconocerse como proveedores de cuidado.
A vida após a morte de Sylvia Plath: mutilação de uma obra, censura de uma vida
Authors: Bezerra Isabella Giordano
Published: August 2023

Resumo: Neste artigo, são levantados os destinos que o nome e a imagem de Sylvia Plath tomaram após sua morte e, para isso, são analisadas as censuras impostas nas publicações póstumas da sua obra. Recorro a três publicações: Ariel, Letters home by Sylvia Plath e The journals of Sylvia Plath. A partir de Rose (2013), Malcolm (2012) e Carvalho (2003), é esclarecido que o objetivo dessas intervenções era oferecer uma imagem conveniente aos responsáveis pelo seu espólio. São discriminados os papéis do público e da crítica na recepção dessas primeiras edições para, enfim, averiguar a impossibilidade de capturar Plath em uma identidade rígida, visto que o uso dos elementos biográficos em sua obra é mais próximo de um processo de subjetivação do que uma fixação em uma verdade única.
INDICADORES SOCIAIS E DE VULNERABILIDADE SOCIAL: CARACTERÍSTICAS E POSSÍVEIS USOS NA AVALIAÇÃO DA COMPETÊNCIA EM INFORMAÇÃO
Authors: Vitorino Elizete Vieira , Pinho Neto Júlio Afonso Sá de
Published: August 2023

RESUMO Este trabalho tem por objetivo identificar possibilidades de usos de indicadores sociais e de vulnerabilidade social na avaliação da Competência em Informação no Brasil. Com abordagem qualitativa e pesquisas bibliográfica e documental, contempla oito temas de indicadores sociais. Os resultados encaminham para os possíveis usos de indicadores sociais em conexão com a Competência em Informação, tomando por base um framework, em três eixos: eixo 1 - conceito de indicadores sociais; conceito de Competência em Informação, conceito de vulnerabilidade social e de vulnerabilidade em informação; eixo 2 - Matriz para o Desenvolvimento da Competência em Informação (PDCIn), nas dimensões técnica, estética, ética e política; virtudes necessárias a indicadores; e, tendências, relativas à avaliação da Competência em Informação e as temáticas a considerar; e, eixo 3 - Agenda 2030 e 17 Objetivos do Desenvolvimento Sustentável (ODS).
Uso do WhatsApp em um grupo de educação em saúde com mulheres
Authors: Ribeiro Ellen Letícia da Silva , Silva Ana Maria Nunes da , Modes Priscilla Shirley Siniak dos Anjos , Marcon Sonia Silva , Oliveira Jeane Cristina Anschau Xavier de , Corrêa Áurea Christina de Paula , Pereira Maira Liliane dos Santos
Published: August 2023

RESUMO Objetivo: Analisar o uso de Tecnologias de Informação e Comunicação e a interação em um grupo de educação em saúde com mulheres no WhatsApp. Metodologia: Pesquisa qualitativa com mães pertencentes a duas Unidades Básicas de Saúde de Sinop - MT incluídas em um Projeto de Extensão que utilizou estratégias educativas em saúde (“Dia G”, “Dicas em saúde” e “Vídeo Educativo”) e membros da equipe do projeto. Os dados foram coletados entre outubro-dezembro de 2021, a partir das mensagens no grupo de WhatsApp e submetidos à Análise de Conteúdo Temática. Resultados: Foram identificadas duas categorias, as quais mostram as informações disponibilizadas pela equipe e as solicitadas pelas mães pontualmente; que as dúvidas manifestadas pelas mães mobilizaram a comunicação-interação entre os participantes e que pouca ou nenhuma interação foi suscitada pelas informações de saúde contidas nas estratégias educativas utilizadas. Considerações finais: O WhatsApp mostrou-se como importante recurso/estratégia na comunicação-interação para educação em saúde com mulheres.
PLANEJAMENTO EDUCACIONAL INDIVIDUALIZADO DE ESTUDANTE COM AUTISMO NA UNIVERSIDADE
Authors: Silveira Victoria Gimenez , Rosa Beatriz Ruiz , Benitez Priscila , Tannús-Valadão Gabriela
Published: August 2023

RESUMO Questiona-se sobre o processo inclusivo universitário de estudantes com Transtorno do Espectro Autista (TEA), visando a permanência e conclusão de curso. O Planejamento Educacional Individualizado (PEI) é uma estratégia de apoio ao estudante com TEA na Educação Básica. O objetivo foi descrever e analisar o desenvolvimento e aplicação do PEI para uma universitária com TEA e comparar desempenho acadêmico antes e depois do PEI. Trata-se de estudo de caso, com avaliações e envolvimento docente. Os resultados descrevem a elaboração e aplicação do PEI com diferenciações na ementa e cronograma das disciplinas cursadas. Anteriormente ao PEI (análise de seis quadrimestres anteriores à pesquisa) a estudante com TEA teve dois trancamentos totais de quadrimestre, quatro reprovações e sete cancelamentos de disciplinas. Após o PEI, análise de três quadrimestres posteriores, a estudante teve um cancelamento de uma disciplina. Os dados evidenciam o PEI enquanto estratégia concreta e eficaz para a permanência da estudante, assim como estratégia viável para formação docente continuada em Educação Especial, no contexto universitário.