bluuebunny/crossref_metadata_2025 huggingface dataset:
Design and Field Tests of an Inverted Based Remote MicroGrid on a Korean Island
Authors: Chae Woo-Kyu , Lee Hak-Ju , Won Jong-Nam , Park Jung-Sung , Kim Jae-Eon
Published: August 2015

In this paper, we present the results of an economic feasibility study and propose a system structure to test and maintain electrical stability. In addition, we present real operation results after constructing a remote microgrid on an island in South Korea. To perform the economic feasibility study, a commercial tool called HOMER was used. The developed remote microgrid consists of a 400 kW wind turbine (WT) generator, 314 kW photovoltaic (PV) generator, 500 kVA × 2 grid forming inverter, 3 MWh lithium ion battery, and an energy management system (EMS). The predicted renewable energy fraction was 91% and real operation result was 82%. The frequency maintaining rate of the diesel power plants was 57% but the remote microgrid was 100%. To improve the operating efficiency of the remote microgrid, we investigated the output range of a diesel generator.
Wind Resource Mapping Using Landscape Roughness and Spatial Interpolation Methods
Authors: Van Ackere Samuel , Van Eetvelde Greet , Schillebeeckx David , Papa Enrica , Van Wyngene Karel , Vandevelde Lieven
Published: August 2015

Energy saving, reduction of greenhouse gasses and increased use of renewables are key policies to achieve the European 2020 targets. In particular, distributed renewable energy sources, integrated with spatial planning, require novel methods to optimise supply and demand. In contrast with large scale wind turbines, small and medium wind turbines (SMWTs) have a less extensive impact on the use of space and the power system, nevertheless, a significant spatial footprint is still present and the need for good spatial planning is a necessity. To optimise the location of SMWTs, detailed knowledge of the spatial distribution of the average wind speed is essential, hence, in this article, wind measurements and roughness maps were used to create a reliable annual mean wind speed map of Flanders at 10 m above the Earth’s surface. Via roughness transformation, the surface wind speed measurements were converted into meso- and macroscale wind data. The data were further processed by using seven different spatial interpolation methods in order to develop regional wind resource maps. Based on statistical analysis, it was found that the transformation into mesoscale wind, in combination with Simple Kriging, was the most adequate method to create reliable maps for decision-making on optimal production sites for SMWTs in Flanders (Belgium).
Competitive Centipede Games: Zero-End Payoffs and Payoff Inequality Deter Reciprocal Cooperation
Authors: Krockow Eva , Pulford Briony , Colman Andrew
Published: August 2015

Reciprocal cooperation can be studied in the Centipede game, in which two players alternate in choosing between a cooperative GO move and a non-cooperative STOP move. GO sustains the interaction and increases the player pair’s total payoff while incurring a small personal cost; STOP terminates the interaction with a favorable payoff to the defector. We investigated cooperation in four Centipede games differing in their payoffs at the game’s end (positive versus zero) and payoff difference between players (moderate versus high difference). The games shared the same game-theoretic solution, therefore they should have elicited identical decision patterns, according to orthodox game theory. Nevertheless, both zero-end payoffs and high payoff inequality were found to reduce cooperation significantly. Contrary to previous predictions, combining these two factors in one game resulted in a slight weakening of their independent deterrent effects. These findings show that small changes in the payoff function have large and significant effects on cooperation, and that the effects do not combine synergistically.
Adaptive Challenges Rising from the Life Context of African-American Caregiving Grandmothers with Diabetes: A Pilot Study
Authors: Carthron Dana , Bailey Donald , Anderson Ruth
Published: August 2015

To understand the challenges arising from the context within which diabetic African-American caregiving grandmothers self-manage their diabetes we used the Adaptive Leadership Framework. Additionally, challenges to retaining this population in a longitudinal study were examined. In this exploratory, longitudinal, qualitative pilot study, data were collected at five time-points over 18 months. We coded the data using content analysis and conducted the within-case and cross-case analyses using data matrices. Lack of awareness of available resources, represented a technical challenge within the life context of these grandmothers and the remaining three themes: family upheaval; priority setting (with subthemes of difficulty meeting basic needs and competing demands); and self-silencing and self-sacrifice represented adaptive challenges. The context of African-American grandmothers’ lives created primarily adaptive challenges that were complex and without immediate solutions. Research is needed to develop culturally and contextually appropriate interventions to help this vulnerable group develop capacity for adaptive work.
Factors associated with Early Initiation of Breastfeeding in Western Nepal
Authors: Khanal Vishnu , Scott Jane , Lee Andy , Karkee Rajendra , Binns Colin
Published: August 2015

The initiation of breastfeeding within one hour of birth has numerous nutritional and immunological benefits and has been found to reduce neonatal mortality. This community-based prospective cohort study aimed to report the rate of, and factors associated with, early initiation of breastfeeding in Western Nepal. The rate of early initiation of breastfeeding was reported, and associations between early initiation and independent variables were tested by Chi-square test, followed by multiple logistic regression. Of the 735 mother-infant pairs, a total of 310 (42.2%) reported early initiation. Mothers who were assisted by traditional attendants during childbirth, delivered by caesarean section, from ethnically disadvantaged families and had delivered low birth weight infants, were less likely to initiate breastfeeding early whereas the mothers who were from the poorest families and did not introduce prelacteal feeds to their infants were more likely to initiate breastfeeding within the first hour. Skills-training to support breastfeeding as part of the training of skilled birth attendants and other health workers is likely to promote recommended infant feeding practices.
Oxidative Stress Mechanisms Caused by Ag Nanoparticles (NM300K) are Different from Those of AgNO3: Effects in the Soil Invertebrate Enchytraeus Crypticus
Authors: Ribeiro Maria , Maria Vera , Scott-Fordsmand Janeck , Amorim Mónica
Published: August 2015

The mechanisms of toxicity of Ag nanoparticles (NPs) are unclear, in particular in the terrestrial environment. In this study the effects of AgNP (AgNM300K) were assessed in terms of oxidative stress in the soil worm Enchytraeus crypticus, using a range of biochemical markers [catalase (CAT), glutathione peroxidase (GPx), glutathione S-transferase (GST), glutathione reductase (GR), total glutathione (TG), metallothionein (MT), lipid peroxidation (LPO)]. E. crypticus were exposed during 3 and 7 days (d) to the reproduction EC20, EC50 and EC80 levels of both AgNP and AgNO3. AgNO3 induced oxidative stress earlier (3 d) than AgNP (7 d), both leading to LPO despite the activation of the anti-redox system. MT increased only for AgNP. The Correspondence Analysis showed a clear separation between AgNO3 and AgNP, with e.g. CAT being the main descriptor for AgNP for 7 d. LPO, GST and GPx were for both 3 and 7 d associated with AgNO3, whereas MT and TG were associated with AgNP. These results may reflect a delay in the effects of AgNP compared to AgNO3 due to the slower release of Ag+ ions from the AgNP, although this does not fully explain the observed differences, i.e., we can conclude that there is a nanoparticle effect.
Assessing the Role of Voluntary Self-Isolation in the Control of Pandemic Influenza Using a Household Epidemic Model
Authors: Zhang Qingxia , Wang Dingcheng
Published: August 2015

In the absence of effective vaccines, antiviral drugs and personal protective measures, such as voluntary self-isolation, have been a part of preparedness plans for the next influenza pandemic. We used a household model to assess the effect of voluntary self-isolation on outbreak control when antiviral drugs are not provided sufficiently early. We found that the early initiation of voluntary self-isolation can overcome the negative effects caused by a delay in antiviral drug distribution when enough symptomatic individuals comply with home confinement at symptom onset. For example, for the baseline household reproduction number RH0 = 2:5, if delays of one or two days occur between clinical symptom development and the start of antiviral prophylaxis, then compliance rates of q ≥ 0:41 and q ≥ 0:6, respectively, are required to achieve the same level of effectiveness as starting antiviral prophylaxis at symptom onset. When the time to beginning voluntary self-isolation after symptom onset increases from zero to two days, this strategy has a limited effect on reducing the transmission of influenza; therefore, this strategy should be implemented as soon as possible. In addition, the effect of voluntary self-isolation decreases substantially with the proportion of asymptomatic infections increasing.
Green Infrastructure, Ecosystem Services, and Human Health
Authors: Coutts Christopher , Hahn Micah
Published: August 2015

Contemporary ecological models of health prominently feature the natural environment as fundamental to the ecosystem services that support human life, health, and well-being. The natural environment encompasses and permeates all other spheres of influence on health. Reviews of the natural environment and health literature have tended, at times intentionally, to focus on a limited subset of ecosystem services as well as health benefits stemming from the presence, and access and exposure to, green infrastructure. The sweeping influence of green infrastructure on the myriad ecosystem services essential to health has therefore often been underrepresented. This survey of the literature aims to provide a more comprehensive picture—in the form of a primer—of the many simultaneously acting health co-benefits of green infrastructure. It is hoped that a more accurately exhaustive list of benefits will not only instigate further research into the health co-benefits of green infrastructure but also promote consilience in the many fields, including public health, that must be involved in the landscape conservation necessary to protect and improve health and well-being.
Monitoring and Prognosis System Based on the ICF for People with Traumatic Brain Injury
Authors: Subirats Laia , Lopez-Blazquez Raquel , Ceccaroni Luigi , Gifre Mariona , Miralles Felip , García-Rudolph Alejandro , Tormos Jose
Published: August 2015

The objective of this research is to provide a standardized platform to monitor and predict indicators of people with traumatic brain injury using the International Classification of Functioning, Disability and Health, and analyze its potential benefits for people with disabilities, health centers and administrations. We developed a platform that allows automatic standardization and automatic graphical representations of indicators of the status of individuals and populations. We used data from 730 people with acquired brain injury performing periodic comprehensive evaluations in the years 2006–2013. Health professionals noted that the use of color-coded graphical representation is useful for quickly diagnose failures, limitations or restrictions in rehabilitation. The prognosis system achieves 41% of accuracy and sensitivity in the prediction of emotional functions, and 48% of accuracy and sensitivity in the prediction of executive functions. This monitoring and prognosis system has the potential to: (1) save costs and time, (2) provide more information to make decisions, (3) promote interoperability, (4) facilitate joint decision-making, and (5) improve policies of socioeconomic evaluation of the burden of disease. Professionals found the monitoring system useful because it generates a more comprehensive understanding of health oriented to the profile of the patients, instead of their diseases and injuries.
Comparative Analysis on Two Schemes for Synthesizing the High Temporal Landsat-like NDVI Dataset Based on the STARFM Algorithm
Authors: Li Ainong , Zhang Wei , Lei Guangbin , Bian Jinhu
Published: August 2015

The NDVI dataset with high temporal and spatial resolution (HTSN) is significant for extracting information about the phenological change of vegetation in regions with a complex earth surface. The Spatial and Temporal Adaptive Reflectance Fusion Model (STARFM) has been successfully applied to synthesize the HTSN by fusing the data with different characteristics. Based on the model, there are two different schemes for synthesizing the HTSN. One scheme is that red reflectance and near-infrared (NIR) reflectance are synthesized, respectively, and the HTSN is then obtained through algebraic operation (Scheme 1); the other scheme is that the red and NIR reflectance are used to calculate NDVI, which is directly taken as input data to synthesize the HTSN (Scheme 2). In this paper, taking the hill areas in eastern Sichuan China as a case, the two schemes were compared with each other. Seven Landsat images and time-series MOD13Q1 datasets spanning from October 2001 to February 2003 were used as the test data. The results showed the prediction accuracies of both derived HTSNs by the two different schemes were generally in good agreement, and Scheme 2 was slightly superior to Scheme 1 (R2: 0.14 < Scheme 1 < 0.53; 0.15 < Scheme 2 < 0.53). Although the two HTSNs showed high temporal and spatial consistence, the small spatiotemporal difference between them had a different influence on different applications. The coincidence rate of cropping intensity extracted from two derived HTSNs was fairly high, reaching up to 93.86%, while the coincidence rate of crop peak dates (i.e., the emerging dates of peaks in an annual time-series NDVI curve) was only 70.95%. Therefore, it is deemed that Scheme 2 can replace Scheme 1 in the application of extracting cropping intensity, so that more calculation time and memory space can be saved. For extracting more quantitative crop phenological information like crop peak dates, more tests are still needed in order to compare the absolute accuracy for both schemes.
Integrating Legal and Physical Dimensions of Urban Environments
Authors: Aien Ali , Rajabifard Abbas , Kalantari Mohsen , Shojaei Davood
Published: August 2015

Building Information Models (e.g., IFC) and virtual 3D city models (e.g., CityGML) are revolutionising the way we manage information about our cities. However, the main focus of these models is on the physical and functional characteristics of urban properties and facilities, which neglects the legal and ownership aspects. In contrast, cadastral data models, such as the Land Administration Domain Model (LADM), have been developed for legal information management purposes and model legal objects such as ownership boundaries without providing correspondence to the object’s physical attributes. Integration of legal and physical objects in the virtual 3D city and cadastral models would maximise their utility and flexibility to support different applications that require an integrated resource of both legal and physical information, such as urban space management and land development processes. The aim of this paper is to propose a data model that supports both legal and physical information of urban environments. The methodology to develop this data model is to extend the core cadastral data model and integrate urban features into the data model. The outcome of the research can be utilised to extend the current data models to increases their usability for different applications that require both legal and physical information.
Stromal Cell-Derived Factor-1α Plays a Crucial Role Based on Neuroprotective Role in Neonatal Brain Injury in Rats
Authors: Mori Miki , Matsubara Keiichi , Matsubara Yuko , Uchikura Yuka , Hashimoto Hisashi , Fujioka Toru , Matsumoto Takashi
Published: August 2015

Owing to progress in perinatal medicine, the survival of preterm newborns has markedly increased. However, the incidence of cerebral palsy has risen in association with increased preterm birth. Cerebral palsy is largely caused by cerebral hypoxic ischemia (HI), for which there are no effective medical treatments. We evaluated the effects of stromal cell-derived factor-1α (SDF-1α) on neonatal brain damage in rats. Left common carotid (LCC) arteries of seven-day-old Wistar rat pups were ligated, and animals were exposed to hypoxic gas to cause cerebral HI. Behavioral tests revealed that the memory and spatial perception abilities were disturbed in HI animals, and that SDF-1α treatment improved these cognitive functions. Motor coordination was also impaired after HI but was unimproved by SDF-1α treatment. SDF-1α reduced intracranial inflammation and induced cerebral remyelination, as indicated by the immunohistochemistry results. These data suggest that SDF-1α specifically influences spatial perception abilities in neonatal HI encephalopathy.
Hepatitis C in Special Patient Cohorts: New Opportunities in Decompensated Liver Cirrhosis, End-Stage Renal Disease and Transplant Medicine
Authors: Hüsing Anna , Kabar Iyad , Schmidt Hartmut , Heinzow Hauke
Published: August 2015

Worldwide, hepatitis C virus (HCV) is a common infection. Due to new antiviral approaches and the approval of direct-acting antiviral agents (DAA), HCV therapy has become more comfortable. Nevertheless, there are special patient groups, in whom treatment of HCV is still challenging. Due to only few data available, tolerability and efficacy of DAAs in special patient cohorts still remain unclear. Such special patient cohorts comprise HCV in patients with decompensated liver disease (Child-Pugh Class B or C), patients with chronic kidney disease, and patients on waiting lists to renal/liver transplantation or those with HCV recurrence after liver transplantation. HCV infection in these patient cohorts has been shown to be associated with increased morbidity and mortality and may lead to reduced graft survival after transplantation. Successful eradication of HCV results in a better outcome concerning liver-related complications and in a better clinical outcome of these patients. In this review, we analyze available data and results from recently published literature and provide an overview of current recommendations of HCV-therapy regimen in these special patient cohorts.
miR-199a and miR-497 Are Associated with Better Overall Survival due to Increased Chemosensitivity in Diffuse Large B-Cell Lymphoma Patients
Authors: Troppan Katharina , Wenzl Kerstin , Pichler Martin , Pursche Beata , Schwarzenbacher Daniela , Feichtinger Julia , Thallinger Gerhard , Beham-Schmid Christine , Neumeister Peter , Deutsch Alexander
Published: August 2015

Micro-RNAs (miRNAs) are short non-coding single-stranded RNA molecules regulating gene expression at the post-transcriptional level. miRNAs are involved in cell development, differentiation, apoptosis, and proliferation. miRNAs can either function as tumor suppressor genes or oncogenes in various important pathways. The expression of specific miRNAs has been identified to correlate with tumor prognosis. For miRNA expression analysis real-time PCR on 81 samples was performed, including 63 diffuse large B-cell lymphoma (DLBCL, 15 of germinal center B-cell like subtype, 17 non germinal center B-cell, 23 transformed, and eight unclassified) and 18 controls, including nine peripheral B-cells, 5 germinal-center B-cells, four lymphadenitis samples, and 4 lymphoma cell lines (RI-1, SUDHL4, Karpas, U2932). Expression levels of a panel of 11 miRNAs that have been previously involved in other types of cancer (miR-15b_2, miR-16_1*, miR-16_2, miR-16_2*, miR-27a, miR-27a*, miR-98-1, miR-103a, miR-185, miR-199a, and miR-497) were measured and correlated with clinical data. Furthermore, cell lines, lacking miR-199a and miR-497 expression, were electroporated with the two respective miRNAs and treated with standard immunochemotherapy routinely used in patients with DLBCL, followed by functional analyses including cell count and apoptosis assays. Seven miRNAs (miR-16_1*, miR-16_2*, miR-27a, miR-103, miR-185, miR-199, and miR-497) were statistically significantly up-regulated in DLBCL compared to normal germinal cells. However, high expression of miR-497 or miR-199a was associated with better overall survival (p = 0.042 and p = 0.007). Overexpression of miR-199a and miR-497 led to a statistically significant decrease in viable cells in a dose-dependent fashion after exposure to rituximab and various chemotherapeutics relevant in multi-agent lymphoma therapy. Our data indicate that elevated miR-199a and miR-497 levels are associated with improved survival in aggressive lymphoma patients most likely by modifying drug sensitivity to immunochemotherapy. This functional impairment may serve as a potential novel therapeutic target in future treatment of patients with DLBCL.
Assessing Transformations of Algal Organic Matter in the Long-Term: Impacts of Humification-Like Processes
Authors: Leloup Maud , Pallier Virginie , Nicolau Rudy , Feuillade-Cathalifaud Geneviève
Published: August 2015

Algae and cyanobacteria are important contributors to the natural organic matter (NOM) of eutrophic water resources. The objective of this work is to increase knowledge on the modifications of algal organic matter (AOM) properties in the long term to anticipate blooms footprint in such aquatic environments. The production of AOM from an alga (Euglena gracilis) and a cyanobacteria (Microcystis aeruginosa) was followed up and characterized during the stationary phase and after one year and four months of cultivation, in batch experiments. Specific UV absorbance (SUVA) index, organic matter fractionation according to hydrophobicity and apparent molecular weight were combined to assess the evolution of AOM. A comparison between humic substances (HS) mainly derived from allochthonous origins and AOM characteristics was performed to hypothesize impacts of AOM transformation processes on the water quality of eutrophic water resources. Each AOM fraction underwent a specific evolution pattern, depending on its composition. Impacts of humification-like processes were predominant over release of biopolymers due to cells decay and led to an increase in the hydrophobic compounds part and molecular weights over time. However, the hydrophilic fraction remained the major fraction whatever the growth stage. Organic compounds generated by maturation of these precursors corresponded to large and aliphatic structures.
UV Differentially Induces Oxidative Stress, DNA Damage and Apoptosis in BCR-ABL1-Positive Cells Sensitive and Resistant to Imatinib
Authors: Synowiec Ewelina , Hoser Grazyna , Wojcik Katarzyna , Pawlowska Elzbieta , Skorski Tomasz , Błasiak Janusz
Published: August 2015

Chronic myeloid leukemia (CML) cells express the active BCR-ABL1 protein, which has been targeted by imatinib in CML therapy, but resistance to this drug is an emerging problem. BCR-ABL1 induces endogenous oxidative stress promoting genomic instability and imatinib resistance. In the present work, we investigated the extent of oxidative stress, DNA damage, apoptosis and expression of apoptosis-related genes in BCR-ABL1 cells sensitive and resistant to imatinib. The resistance resulted either from the Y253H mutation in the BCR-ABL1 gene or incubation in increasing concentrations of imatinib (AR). UV irradiation at a dose rate of 0.12 J/(m2·s) induced more DNA damage detected by the T4 pyrimidine dimers glycosylase and hOGG1, recognizing oxidative modifications to DNA bases in imatinib-resistant than -sensitive cells. The resistant cells displayed also higher susceptibility to UV-induced apoptosis. These cells had lower native mitochondrial membrane potential than imatinib-sensitive cells, but UV-irradiation reversed that relationship. We observed a significant lowering of the expression of the succinate dehydrogenase (SDHB) gene, encoding a component of the complex II of the mitochondrial respiratory chain, which is involved in apoptosis sensing. Although detailed mechanism of imatinib resistance in AR cells in unknown, we detected the presence of the Y253H mutation in a fraction of these cells. In conclusion, imatinib-resistant cells may display a different extent of genome instability than their imatinib-sensitive counterparts, which may follow their different reactions to both endogenous and exogenous DNA-damaging factors, including DNA repair and apoptosis.
Neurological and Epigenetic Implications of Nutritional Deficiencies on Psychopathology: Conceptualization and Review of Evidence
Authors: Liu Jianghong , Zhao Sophie , Reyes Teresa
Published: August 2015

In recent years, a role for epigenetic modifications in the pathophysiology of disease has received significant attention. Many studies are now beginning to explore the gene–environment interactions, which may mediate early-life exposure to risk factors, such as nutritional deficiencies and later development of behavioral problems in children and adults. In this paper, we review the current literature on the role of epigenetics in the development of psychopathology, with a specific focus on the potential for epigenetic modifications to link nutrition and brain development. We propose a conceptual framework whereby epigenetic modifications (e.g., DNA methylation) mediate the link between micro- and macro-nutrient deficiency early in life and brain dysfunction (e.g., structural aberration, neurotransmitter perturbation), which has been linked to development of behavior problems later on in life.
Synthesis and in Vitro Antifungal Activity against Botrytis cinerea of Geranylated Phenols and Their Phenyl Acetate Derivatives
Authors: Chávez María , Soto Mauricio , Taborga Lautaro , Díaz Katy , Olea Andrés , Bay Camila , Peña-Cortés Hugo , Espinoza Luis
Published: August 2015

The inhibitory effects on the mycelial growth of plant pathogen Botritys cinerea have been evaluated for a series of geranylphenols substituted with one, two and three methoxy groups in the aromatic ring. The results show that the antifungal activity depends on the structure of the geranylphenols, increasing from 40% to 90% by increasing the number of methoxy groups. On the other hand, the acetylation of the –OH group induces a change of activity that depends on the number of methoxy groups. The biological activity of digeranyl derivatives is lower than that exhibited by the respective monogeranyl compound. All tested geranylphenols have been synthesized by direct coupling of geraniol and the respective phenol. The effect of solvent on yields and product distribution is discussed. For monomethoxyphenols the reaction gives better yields when acetonitrile is used as a solvent and AgNO3 is used as a secondary catalyst. However, for di- and trimethoxyphenols the reaction proceeds only in dioxane.
Structure-Based Optimization of Inhibitors of the Aspartic Protease Endothiapepsin
Authors: Hartman Alwin , Mondal Milon , Radeva Nedyalka , Klebe Gerhard , Hirsch Anna
Published: August 2015

Aspartic proteases are a class of enzymes that play a causative role in numerous diseases such as malaria (plasmepsins), Alzheimer’s disease (β-secretase), fungal infections (secreted aspartic proteases), and hypertension (renin). We have chosen endothiapepsin as a model enzyme of this class of enzymes, for the design, preparation and biochemical evaluation of a new series of inhibitors of endothiapepsin. Here, we have optimized a hit, identified by de novo structure-based drug design (SBDD) and DCC, by using structure-based design approaches focusing on the optimization of an amide–π interaction. Biochemical results are in agreement with SBDD. These results will provide useful insights for future structure-based optimization of inhibitors for the real drug targets as well as insights into molecular recognition.
Gene Networks Involved in Hormonal Control of Root Development in Arabidopsis thaliana: A Framework for Studying Its Disturbance by Metal Stress
Authors: De Smet Stefanie , Cuypers Ann , Vangronsveld Jaco , Remans Tony
Published: August 2015

Plant survival under abiotic stress conditions requires morphological and physiological adaptations. Adverse soil conditions directly affect root development, although the underlying mechanisms remain largely to be discovered. Plant hormones regulate normal root growth and mediate root morphological responses to abiotic stress. Hormone synthesis, signal transduction, perception and cross-talk create a complex network in which metal stress can interfere, resulting in root growth alterations. We focus on Arabidopsis thaliana, for which gene networks in root development have been intensively studied, and supply essential terminology of anatomy and growth of roots. Knowledge of gene networks, mechanisms and interactions related to the role of plant hormones is reviewed. Most knowledge has been generated for auxin, the best-studied hormone with a pronounced primary role in root development. Furthermore, cytokinins, gibberellins, abscisic acid, ethylene, jasmonic acid, strigolactones, brassinosteroids and salicylic acid are discussed. Interactions between hormones that are of potential importance for root growth are described. This creates a framework that can be used for investigating the impact of abiotic stress factors on molecular mechanisms related to plant hormones, with the limited knowledge of the effects of the metals cadmium, copper and zinc on plant hormones and root development included as case example.
Tangential Flow Ultrafiltration Allows Purification and Concentration of Lauric Acid-/Albumin-Coated Particles for Improved Magnetic Treatment
Authors: Zaloga Jan , Stapf Marcus , Nowak Johannes , Pöttler Marina , Friedrich Ralf , Tietze Rainer , Lyer Stefan , Lee Geoffrey , Odenbach Stefan , Hilger Ingrid , Alexiou Christoph
Published: August 2015

Superparamagnetic iron oxide nanoparticles (SPIONs) are frequently used for drug targeting, hyperthermia and other biomedical purposes. Recently, we have reported the synthesis of lauric acid-/albumin-coated iron oxide nanoparticles SEONLA-BSA, which were synthesized using excess albumin. For optimization of magnetic treatment applications, SPION suspensions need to be purified of excess surfactant and concentrated. Conventional methods for the purification and concentration of such ferrofluids often involve high shear stress and low purification rates for macromolecules, like albumin. In this work, removal of albumin by low shear stress tangential ultrafiltration and its influence on SEONLA-BSA particles was studied. Hydrodynamic size, surface properties and, consequently, colloidal stability of the nanoparticles remained unchanged by filtration or concentration up to four-fold (v/v). Thereby, the saturation magnetization of the suspension can be increased from 446.5 A/m up to 1667.9 A/m. In vitro analysis revealed that cellular uptake of SEONLA-BSA changed only marginally. The specific absorption rate (SAR) was not greatly affected by concentration. In contrast, the maximum temperature Tmax in magnetic hyperthermia is greatly enhanced from 44.4 °C up to 64.9 °C by the concentration of the particles up to 16.9 mg/mL total iron. Taken together, tangential ultrafiltration is feasible for purifying and concentrating complex hybrid coated SPION suspensions without negatively influencing specific particle characteristics. This enhances their potential for magnetic treatment.
Identification of Proteins Modulated in the Date Palm Stem Infested with Red Palm Weevil (Rhynchophorus ferrugineus Oliv.) Using Two Dimensional Differential Gel Electrophoresis and Mass Spectrometry
Authors: Rasool Khawaja , Khan Muhammad , Aldawood Abdulrahman , Tufail Muhammad , Mukhtar Muhammad , Takeda Makio
Published: August 2015

A state of the art proteomic methodology using Matrix Assisted Laser Desorption/Ionization-Time of Flight (MALDI TOF) has been employed to characterize peptides modulated in the date palm stem subsequent to infestation with red palm weevil (RPW). Our analyses revealed 32 differentially expressed peptides associated with RPW infestation in date palm stem. To identify RPW infestation associated peptides (I), artificially wounded plants (W) were used as additional control beside uninfested plants, a conventional control (C). A constant unique pattern of differential expression in infested (I), wounded (W) stem samples compared to control (C) was observed. The upregulated proteins showed relative fold intensity in order of I > W and downregulated spots trend as W > I, a quite interesting pattern. This study also reveals that artificially wounding of date palm stem affects almost the same proteins as infestation; however, relative intensity is quite lower than in infested samples both in up and downregulated spots. All 32 differentially expressed spots were subjected to MALDI-TOF analysis for their identification and we were able to match 21 proteins in the already existing databases. Relatively significant modulated expression pattern of a number of peptides in infested plants predicts the possibility of developing a quick and reliable molecular methodology for detecting plants infested with date palm.
Pyrosequencing-Based Assays for Rapid Detection of HER2 and HER3 Mutations in Clinical Samples Uncover an E332E Mutation Affecting HER3 in Retroperitoneal Leiomyosarcoma
Authors: González-Alonso Paula , Chamizo Cristina , Moreno Víctor , Madoz-Gúrpide Juan , Carvajal Nerea , Daoud Lina , Zazo Sandra , Martín-Aparicio Ester , Cristóbal Ion , Rincón Raúl , García-Foncillas Jesús , Rojo Federico
Published: August 2015

Mutations in Human Epidermal Growth Factor Receptors (HER) are associated with poor prognosis of several types of solid tumors. Although HER-mutation detection methods are currently available, such as Next-Generation Sequencing (NGS), alternative pyrosequencing allow the rapid characterization of specific mutations. We developed specific PCR-based pyrosequencing assays for identification of most prevalent HER2 and HER3 mutations, including S310F/Y, R678Q, L755M/P/S/W, V777A/L/M, 774-776 insertion, and V842I mutations in HER2, as well as M91I, V104M/L, D297N/V/Y, and E332E/K mutations in HER3. We tested 85 Formalin Fixed and Paraffin Embbeded (FFPE) samples and we detected three HER2-V842I mutations in colorectal carcinoma (CRC), ovarian carcinoma, and pancreatic carcinoma patients, respectively, and a HER2-L755M mutation in a CRC specimen. We also determined the presence of a HER3-E332K mutation in an urothelial carcinoma sample, and two HER3-D297Y mutations, in both gastric adenocarcinoma and CRC specimens. The D297Y mutation was previously detected in breast and gastric tumors, but not in CRC. Moreover, we found a not-previously-described HER3-E332E synonymous mutation in a retroperitoneal leiomyosarcoma patient. The pyrosequencing assays presented here allow the detection and characterization of specific HER2 and HER3 mutations. These pyrosequencing assays might be implemented in routine diagnosis for molecular characterization of HER2/HER3 receptors as an alternative to complex NGS approaches.
Exploring miRNA-Associated Signatures with Diagnostic Relevance in Glioblastoma Multiforme and Breast Cancer Patients
Authors: LeBlanc Véronique , Morin Pier
Published: August 2015

The growing attention that non-coding RNAs have attracted in the field of cancer research in recent years is undeniable. Whether investigated as prospective therapeutic targets or prognostic indicators or diagnostic biomarkers, the clinical relevance of these molecules is starting to emerge. In addition, identification of non-coding RNAs in a plethora of body fluids has further positioned these molecules as attractive non-invasive biomarkers. This review will first provide an overview of the synthetic cascade that leads to the production of the small non-coding RNAs microRNAs (miRNAs) and presents their strengths as biomarkers of disease. Our interest will next be directed at exploring the diagnostic utility of miRNAs in two types of cancer: the brain tumor glioblastoma multiforme (GBM) and breast cancer. Finally, we will discuss additional clinical implications associated with miRNA detection as well as introduce other non-coding RNAs that have generated recent interest in the cancer research community.
Volatility Forecast in Crises and Expansions
Authors: Pypko Sergii
Published: August 2015

We build a discrete-time non-linear model for volatility forecasting purposes. This model belongs to the class of threshold-autoregressive models, where changes in regimes are governed by past returns. The ability to capture changes in volatility regimes and using more accurate volatility measures allow outperforming other benchmark models, such as linear heterogeneous autoregressive model and GARCH specifications. Finally, we show how to derive closed-form expression for multiple-step-ahead forecasting by exploiting information about the conditional distribution of returns.
Nanocarbon-Coated Porous Anodic Alumina for Bionic Devices
Authors: Aramesh Morteza , Tong Wei , Fox Kate , Turnley Ann , Seo Dong , Prawer Steven , Ostrikov Kostya
Published: August 2015

A highly-stable and biocompatible nanoporous electrode is demonstrated herein. The electrode is based on a porous anodic alumina which is conformally coated with an ultra-thin layer of diamond-like carbon. The nanocarbon coating plays an essential role for the chemical stability and biocompatibility of the electrodes; thus, the coated electrodes are ideally suited for biomedical applications. The corrosion resistance of the proposed electrodes was tested under extreme chemical conditions, such as in boiling acidic/alkali environments. The nanostructured morphology and the surface chemistry of the electrodes were maintained after wet/dry chemical corrosion tests. The non-cytotoxicity of the electrodes was tested by standard toxicity tests using mouse fibroblasts and cortical neurons. Furthermore, the cell–electrode interaction of cortical neurons with nanocarbon coated nanoporous anodic alumina was studied in vitro. Cortical neurons were found to attach and spread to the nanocarbon coated electrodes without using additional biomolecules, whilst no cell attachment was observed on the surface of the bare anodic alumina. Neurite growth appeared to be sensitive to nanotopographical features of the electrodes. The proposed electrodes show a great promise for practical applications such as retinal prostheses and bionic implants in general.
Capacitive Behavior of Single Gallium Oxide Nanobelt
Authors: Cai Haitao , Liu Hang , Zhu Huichao , Shao Pai , Hou Changmin
Published: August 2015

In this research, monocrystalline gallium oxide (Ga2O3) nanobelts were synthesized through oxidation of metal gallium at high temperature. An electronic device, based on an individual Ga2O3 nanobelt on Pt interdigital electrodes (IDEs), was fabricated to investigate the electrical characteristics of the Ga2O3 nanobelt in a dry atmosphere at room temperature. The current-voltage (I-V) and I/V-t characteristics show the capacitive behavior of the Ga2O3 nanobelt, indicating the existence of capacitive elements in the Pt/Ga2O3/Pt structure.
Move, Hold and Touch: A Framework for Tangible Gesture Interactive Systems
Authors: Angelini Leonardo , Lalanne Denis , Hoven Elise , Khaled Omar , Mugellini Elena
Published: August 2015

Technology is spreading in our everyday world, and digital interaction beyond the screen, with real objects, allows taking advantage of our natural manipulative and communicative skills. Tangible gesture interaction takes advantage of these skills by bridging two popular domains in Human-Computer Interaction, tangible interaction and gestural interaction. In this paper, we present the Tangible Gesture Interaction Framework (TGIF) for classifying and guiding works in this field. We propose a classification of gestures according to three relationships with objects: move, hold and touch. Following this classification, we analyzed previous work in the literature to obtain guidelines and common practices for designing and building new tangible gesture interactive systems. We describe four interactive systems as application examples of the TGIF guidelines and we discuss the descriptive, evaluative and generative power of TGIF.
Chitosan: An Update on Potential Biomedical and Pharmaceutical Applications
Authors: Cheung Randy , Ng Tzi , Wong Jack , Chan Wai
Published: August 2015

Chitosan is a natural polycationic linear polysaccharide derived from chitin. The low solubility of chitosan in neutral and alkaline solution limits its application. Nevertheless, chemical modification into composites or hydrogels brings to it new functional properties for different applications. Chitosans are recognized as versatile biomaterials because of their non-toxicity, low allergenicity, biocompatibility and biodegradability. This review presents the recent research, trends and prospects in chitosan. Some special pharmaceutical and biomedical applications are also highlighted.
Rare Chromones from a Fungal Mutant of the Marine-Derived Penicillium purpurogenum G59
Authors: Xia Ming-Wen , Cui Cheng-Bin , Li Chang-Wei , Wu Chang-Jing , Peng Ji-Xing , Li De-Hai
Published: August 2015

Three new and rare chromones, named epiremisporine B (2), epiremisporine B1 (3) and isoconiochaetone C (4), along with three known remisporine B (1), coniochaetone A (5) and methyl 8-hydroxy-6-methyl-9-oxo-9H-xanthene-1-carboxylate (6) were isolated from a mutant from the diethyl sulfate (DES) mutagenesis of a marine-derived Penicillium purpurogenum G59. The structures of 2–4 including the absolute configurations were determined by spectroscopic methods, especially by NMR analysis and electronic circular dichroism (ECD) experiments in conjunction with calculations. The absolute configuration of the known remisporine B (1) was determined for the first time. Compounds 2 and 3 have a rare feature that has only been reported in one example so far. The compounds 1–6 were evaluated for their cytotoxicity against several human cancer cell lines. The present work explored the great potential of our previous DES mutagenesis strategy for activating silent fungal pathways, which has accelerated the discovery of new bioactive compounds.
Material Properties of Various Cast Aluminum Alloys Made Using a Heated Mold Continuous Casting Technique with and without Ultrasonic Vibration
Authors: Okayasu Mitsuhiro , Miyamoto Yuta , Morinaka Kazuma
Published: August 2015

This work was carried out to develop high-quality cast aluminum alloys using a new casting technology. For this purpose, commercial Al alloys were created by heated mold continuous casting (HMC) with ultrasonic vibration (UV). With the HMC process, the grain size and the crystal orientation of the Al alloys were controlled, i.e., fine grains with a uniformly organized lattice formation. In addition, an attempt was made to modify the microstructural formation by cavitation. These microstructural characteristics made excellent mechanical properties. Using UV in the continuous casting process, more fine and spherical grains were slightly disordered, which was detected using electron backscattered diffraction. The mechanical properties of the UV HMC Al alloys were slightly higher than those for the related cast Al alloys without UV. Moreover, the severe vibration caused higher mechanical properties. The lattice and dislocation characteristics of the cast samples made with and without UV processes were analyzed systematically using electron backscattered diffraction.
Water Impingement Erosion of Deep-Rolled Ti64
Authors: Ma Dina , Mostafa Ahmad , Kevorkov Dmytro , Jedrzejowski Pawel , Pugh Martin , Medraj Mamoun
Published: August 2015

In this work, the Liquid Impingement Erosion (LIE) performances of deep-rolling (DR) treated and non-treated Ti64 were investigated. Various erosion stages, from the incubation to the terminal erosion stages, could be observed. A full factorial design of experiments was used to study the effect of DR process parameters (Feed Rate, Spindle Velocity, Number of Passes, Pressure) on the residual stress distribution, microhardness and surface roughness of the treated Ti64 specimens. The DR-treated Ti64 specimens exhibited improved surface microhardness, surface roughness, and large magnitude of compressive residual stresses, which were attributed to the amount of cold work induced by the DR process. Although DR improved the mechanical properties of the Ti64, the results showed that the treatment has little or no effect on the LIE performance of Ti64 but different damage modes were observed in these two cases. Evolution of the erosion stages was described based on water-hammer pressure, stress waves, radial wall jetting, and hydraulic penetration modes. The initial erosion stages were mainly influenced by water-hammer pressure and stress waves, whereas the intermediate erosion stages were influenced by the combination of the four modes together. The final erosion stages contain the four modes, however the erosion was greatly driven by the radial jetting and hydraulic penetration modes, where more material was removed. The failure mechanism of the final stages of the LIE test of both DR-treated and non-treated Ti64 was characterized as fatigue fracture. However, a brittle fracture behavior was observed in the initial and intermediate erosion stages of the DR-treated Ti64, whereas a ductile fracture behavior was observed in the non-treated Ti64. This was concluded from the micrographs of the LIE damage through different erosion stages.
Insect-Inspired Micropump: Flow in a Tube with Local Contractions
Authors: Aboelkassem Yasser
Published: August 2015

A biologically-inspired micropumping model in a three-dimensional tube subjected to localized wall constrictions is given in this article. The present study extends our previous pumping model where a 3D channel with a square cross-section is considered. The proposed pumping approach herein applies to tubular geometries and is given to mimic an insect respiration mode, where the tracheal tube rhythmic wall contractions are used/hypothesized to enhance the internal flow transport within the entire respiration network. The method of regularized Stokeslets-mesh-free computations is used to reconstruct the flow motions induced by the wall movements and to calculate the time-averaged net flow rate. The time-averaged net flow rates from both the tube and channel models are compared. Results have shown that an inelastic tube with at least two contractions forced to move with a specific time lag protocol can work as a micropump. The system is simple and expected to be useful in many biomedical applications.