bluuebunny/crossref_metadata_2025 huggingface dataset:
Freshwater wetland–driven variation in sulfur isotope compositions: Implications for human paleodiet and ecological research
Authors: Guiry Eric J. , Orchard Trevor J. , Needs-Howarth Suzanne , Szpak Paul
Published: December 2022

Sulfur isotope (δ34S) analyses are an important archaeological and ecological tool for understanding human and animal migration and diet, but δ34S can be difficult to interpret, particularly in archaeological human-mobility studies, when measured isotope compositions are strongly 34S-depleted relative to regional baselines. Sulfides, which accumulate under anoxic conditions and have distinctively low δ34S, are potentially key for understanding this but are often overlooked in studies of vertebrate δ34S. We analyze an ecologically wide range of archaeological taxa to build an interpretive framework for understanding the impact of sulfide-influenced δ34S on vertebrate consumers. Results provide the first demonstration that δ34S of higher-level consumers can be heavily impacted by freshwater wetland resource use. This source of δ34S variation is significant because it is linked to a globally distributed habitat and occurs at the bottom of the δ34S spectrum, which, for archaeologists, is primarily used for assessing human mobility. Our findings have significant implications for rethinking traditional interpretive frameworks of human mobility and diet, and for exploring the historical ecology of past freshwater wetland ecosystems. Given the tremendous importance of wetlands’ ecosystem services today, such insights on the structure and human dynamics of past wetlands could be valuable for guiding restoration work.
Divergent response of Pinus pumila growth to climate warming at different latitudes and in different simulation predictions
Authors: Yang Jingwen , Zhang Qiuliang , Song Wenqi , An Yang , Wang Xiaochun
Published: December 2022

Understanding the responses of shrub growth to climate changes is key to accurately predicting the dynamic changes in the boreal forest ecosystem. A shrub-ring network based on 12 sites was established to explore the response of Pinus pumila growth to climate change in northeastern China. The sampling sites are distributed along the latitudinal gradient of 44.10° to 52.58° and were divided into three regions: south, central, and north. The results show that there have been significant differences in P. pumila growth trends at different latitudes in recent decades. From 1950 to 1980, P. pumila in the southern and central regions grew faster than in the northern region. From 1981 to 2014, however, the growth of P. pumila increased in the north but slowed in the south and central regions. The temperatures of the previous winter and current spring are the main factors limiting the growth of P. pumila in the north and south. In the central and southern regions, the growth of P. pumila is negatively correlated with precipitation of the previous and current summers. In the north, summer temperatures from 1981 to 2014 gradually inhibit the growth of P. pumila, while summer precipitation has a positive impact on growth. Our model indicates that the radial growth trend of P. pumila in the north is likely to decline as the climate warms. Surprisingly, the growth of P. pumila in the south is predicted to benefit from warming under the RCP4.5, RCP6.0, and RCP8.5 scenarios. Therefore, P. pumila may not expand northward in the context of climate warming.
Determinants of long COVID among adults hospitalized for SARS-CoV-2 infection: A prospective cohort study
Authors: Bellan Mattia , Apostolo Daria , Albè Alice , Crevola Martina , Errica Nicolò , Ratano Giacomo , Tonello Stelvio , Minisini Rosalba , D’Onghia Davide , Baricich Alessio , Patrucco Filippo , Zeppegno Patrizia , Gramaglia Carla , Balbo Piero Emilio , Cappellano Giuseppe , Casella Sara , Chiocchetti Annalisa , Clivati Elisa , Giordano Mara , Manfredi Marcello , Patti Giuseppe , Pinato David James , Puricelli Chiara , Raineri Davide , Rolla Roberta , Sainaghi Pier Paolo , Pirisi Mario ,   the No-More COVID study group
Published: December 2022

Rationale

Factors associated with long-term sequelae emerging after the acute phase of COVID-19 (so called “long COVID”) are unclear. Here, we aimed to identify risk factors for the development of COVID-19 sequelae in a prospective cohort of subjects hospitalized for SARS-CoV-2 infection and followed up one year after discharge.

Methods

A total of 324 subjects underwent a comprehensive and multidisciplinary evaluation one year after hospital discharge for COVID-19. A subgroup of 247/324 who consented to donate a blood sample were tested for a panel of circulating cytokines.

Results

In 122 patients (37.8%) there was evidence of at least one persisting physical symptom. After correcting for comorbidities and COVID-19 severity, the risk of developing long COVID was lower in the 109 subjects admitted to the hospital in the third wave of the pandemic than in the 215 admitted during the first wave, (OR 0.69, 95%CI 0.51-0.93, p=0.01). Univariable analysis revealed female sex, diffusing capacity of the lungs for carbon monoxide (DLCO) value, body mass index, anxiety and depressive symptoms to be positively associated with COVID-19 sequelae at 1 year. Following logistic regression analysis, DLCO was the only independent predictor of residual symptoms (OR 0.98 CI 95% (0.96-0.99), p=0.01). In the subgroup of subjects with normal DLCO (> 80%), for whom residual lung damage was an unlikely explanation for long COVID, the presence of anxiety and depressive symptoms was significantly associated to persistent symptoms, together with increased levels of a set of pro-inflammatory cytokines: interferon-gamma, tumor necrosis factor-alpha, interleukin (IL)-2, IL-12, IL-1β, IL-17. In logistic regression analysis, depressive symptoms (p=0.02, OR 4.57 [1.21-17.21]) and IL-12 levels (p=0.03, OR 1.06 [1.00-1.11]) 1-year after hospital discharge were independently associated with persistence of symptoms.

Conclusions

Long COVID appears mainly related to respiratory sequelae, prevalently observed during the first pandemic wave. Among patients with little or no residual lung damage, a cytokine pattern consistent with systemic inflammation is in place.
Therapeutic potential of paeoniflorin in atherosclerosis: A cellular action and mechanism-based perspective
Authors: Yu Wei , Ilyas Iqra , Hu Xuerui , Xu Suowen , Yu Hui
Published: December 2022

Epidemiological studies have shown that the incidence, prevalence and mortality of atherosclerotic cardiovascular disease (ASCVD) are increasing globally. Atherosclerosis is characterized as a chronic inflammatory disease which involves inflammation and immune dysfunction. P. lactiflora Pall. is a plant origin traditional medicine that has been widely used for the treatment of various diseases for more than a millennium in China, Japan and Korean. Paeoniflorin is a bioactive monomer extracted from P. lactiflora Pall. with anti-atherosclerosis effects. In this article, we comprehensively reviewed the potential therapeutic effects and molecular mechanism whereby paeoniflorin protects against atherosclerosis from the unique angle of inflammation and immune-related pathway dysfunction in vascular endothelial cells, smooth muscle cells, monocytes, macrophages, platelets and mast cells. Paeoniflorin, with multiple protective effects in atherosclerosis, has the potential to be used as a promising therapeutic agent for the treatment of atherosclerosis and its complications. We conclude with a detailed discussion of the challenges and future perspective of paeoniflorin in translational cardiovascular medicine.
Effects of replacing fishmeal with cottonseed protein concentrate on growth performance, blood metabolites, and the intestinal health of juvenile rainbow trout (Oncorhynchus mykiss)
Authors: Liu Yang , Ma Shuwei , Lv Weihua , Shi Honghe , Qiu Guangwen , Chang Hongmiao , Lu Shaoxia , Wang Di , Wang Changan , Han Shicheng , Liu Hongbai
Published: December 2022

Cottonseed protein concentrate (CPC) is a potential non-food protein source for fishmeal replacement in fish feed. However, a high inclusion level of CPC in diets may have adverse effects on the metabolism and health of carnivorous fish. This study aimed to investigate CPC as a fishmeal alternative in the diet of rainbow trout Oncorhynchus mykiss based on growth performance, blood metabolites, and intestinal health. Five isonitrogenous (46% crude protein) and isolipidic (16% crude lipid) diets were formulated: a control diet (30% fishmeal) and four experimental diets with substitution of fishmeal by CPC at 25%, 50%, 75%, and 100%. A total of 600 fish (mean body weight 11.24g) were hand-fed the five formulated diets to apparent satiation for eight weeks. The results showed no adverse effects on growth performance when 75% dietary fishmeal was replaced by CPC. However, reduced growth and feed intake were observed in rainbow trout fed a fishmeal-free diet based on CPC (CPC100%). Changes in serum metabolites were also observed in CPC100% compared with the control group, including an increase in alanine aminotransferase (ALT), a decrease in alkaline phosphatase (ALP), alterations in free amino acids, and reductions in cholesterol metabolism. In addition, the CPC-based diet resulted in reduced intestinal trypsin, decreased villus height and width in the distal intestine, upregulated mRNA expression levels of inflammatory cytokines in the intestine, and impaired gut microbiota with reduced bacterial diversity and decreased abundance of Bacillaceae compared with the control group. The findings suggest that the optimum substitution rate of dietary fishmeal by CPC for rainbow trout should be less than 75%.
Methotrexate inhibition of SARS-CoV-2 entry, infection and inflammation revealed by bioinformatics approach and a hamster model
Authors: Chen Yun-Ti , Chang Yu-Hsiu , Pathak Nikhil , Tzou Shey-Cherng , Luo Yong-Chun , Hsu Yen-Chao , Li Tian-Neng , Lee Jung-Yu , Chen Yi-Cyun , Huang Yu-Wei , Yang Hsin-Ju , Hsu Nung-Yu , Tsai Hui-Ping , Chang Tein-Yao , Hsu Shu-Chen , Liu Ping-Cheng , Chin Yuan-Fan , Lin Wen-Chin , Yang Chuen-Mi , Wu Hsueh-Ling , Lee Chia-Ying , Hsu Hui-Ling , Liu Yi-Chun , Chu Jhih-Wei , Wang Lily Hui-Ching , Wang Jann-Yuan , Huang Chih-Heng , Lin Chi-Hung , Hsieh Po-Shiuan , Wu Lee Yan-Hwa , Hung Yi-Jen , Yang Jinn-Moon
Published: December 2022

Background

Drug repurposing is a fast and effective way to develop drugs for an emerging disease such as COVID-19. The main challenges of effective drug repurposing are the discoveries of the right therapeutic targets and the right drugs for combating the disease.

Methods

Here, we present a systematic repurposing approach, combining Homopharma and hierarchal systems biology networks (HiSBiN), to predict 327 therapeutic targets and 21,233 drug-target interactions of 1,592 FDA drugs for COVID-19. Among these multi-target drugs, eight candidates (along with pimozide and valsartan) were tested and methotrexate was identified to affect 14 therapeutic targets suppressing SARS-CoV-2 entry, viral replication, and COVID-19 pathologies. Through the use of in vitro (EC50 = 0.4 μM) and in vivo models, we show that methotrexate is able to inhibit COVID-19 via multiple mechanisms.

Results

Our in vitro studies illustrate that methotrexate can suppress SARS-CoV-2 entry and replication by targeting furin and DHFR of the host, respectively. Additionally, methotrexate inhibits all four SARS-CoV-2 variants of concern. In a Syrian hamster model for COVID-19, methotrexate reduced virus replication, inflammation in the infected lungs. By analysis of transcriptomic analysis of collected samples from hamster lung, we uncovered that neutrophil infiltration and the pathways of innate immune response, adaptive immune response and thrombosis are modulated in the treated animals.

Conclusions

We demonstrate that this systematic repurposing approach is potentially useful to identify pharmaceutical targets, multi-target drugs and regulated pathways for a complex disease. Our findings indicate that methotrexate is established as a promising drug against SARS-CoV-2 variants and can be used to treat lung damage and inflammation in COVID-19, warranting future evaluation in clinical trials.
Pan-cancer analysis of ASB3 and the potential clinical implications for immune microenvironment of glioblastoma multiforme
Authors: Mu Long , Han Zhibin , Yu Shengkun , Wang Aowen , Chen Dongjiang , Kong Sijia , Gu Yifei , Xu Lin , Liu Axiang , Sun Ruohan , Long Yu
Published: December 2022

Background

Ankyrin repeat and SOCS Box containing 3 (ASB3) is an E3 ubiquitin ligase. It has been reported to regulate the progression of some cancers, but no systematic pan-cancer analysis has been conducted to explore its function in prognosis and immune microenvironment.

Method

In this study, mRNA expression data were downloaded from TCGA and GTEx database. Next generation sequencing data from 14 glioblastoma multiforme (GBM) samples by neurosurgical resection were used as validation dataset. Multiple bioinformatics methods (ssGSEA, Kaplan-Meier, Cox regression analysis, GSEA and online tools) were applied to explore ASB3 expression, gene activity, prognosis of patients in various cancers, and its correlation with clinical information, immune microenvironment and pertinent signal pathways in GBM. The biological function of ASB3 in tumor-infiltrating lymphocytes (TILs) was verified using an animal model.

Results

We found that ASB3 was aberrant expressed in a variety of tumors, especially in GBM, and significantly correlated with the prognosis of cancer patients. The level of ASB3 was related to the TMB, MSI and immune cell infiltration in some cancer types. ASB3 had a negative association with immune infiltration and TME, including regulatory T cells (Tregs), cancer-associated fibroblasts, immunosuppressors and related signaling pathways in GBM. ASB3 overexpression reduced the proportion of Tregs in TILs. GSEA and PPI analysis also showed negative correlation between ASB3 expression and oncogenetic signaling pathways in GBM.

Conclusion

A comprehensive pan-cancer analysis of ASB3 showed its potential function as a biomarker of cancer prognosis and effective prediction of immunotherapy response. This study not only enriches the understanding of the biological function of ASB3 in pan-cancer, especially in GBM immunity, but also provides a new reference for the personalized immunotherapy of GBM.
Effects of sea ice retreat and ocean warming on the Laptev Sea continental slope ecosystem (1993 vs 2012)
Authors: Bienhold Christina , Schourup-Kristensen Vibe , Krumpen Thomas , Nöthig Eva-Maria , Wenzhöfer Frank , Korhonen Meri , Vredenborg Myriel , Hehemann Laura , Boetius Antje
Published: December 2022

The central Arctic Ocean is rapidly changing due to amplified warming and sea ice retreat. Nonetheless, it remains challenging to document and decipher impacts on key ecosystem processes such as primary production and pelagic-benthic coupling, due to limited observations in this remote area. Here we investigated environmental changes at the Laptev Sea continental slope (60-3400 m water depth) from the surface to the seafloor, by replicating sample transects two decades apart. Mean break-up of sea ice occurred earlier and mean freeze-up occurred later in 2012 compared to 1993, extending the ice-free period by more than 30 days. On average, observations and model results showed an annual increase in primary production of 30% and more in the study area in 2012. In contrast, calculated and modelled fluxes of particulate organic carbon (POC) to the seafloor were only slightly higher in 2012 and did not extend as far into the deep Laptev Sea as the increase in primary production, possibly due to a more developed retention system. Nevertheless, benthic surveys revealed a substantial increase in phytodetritus availability at the seafloor along the entire transect from the shelf edge to the deep sea. This calls for carbon input by lateral advection from the shelves, additional input from sea ice, and/or a late summer bloom. We also investigated the composition and activity of bacterial communities at the seafloor and potential linkages to the observed environmental changes. While bacterial abundance, biomass and overall community structure showed no systematic differences between the two contrasting years at all depths, extracellular enzymatic activities had increased as a result of higher food availability. This was partly reflected in higher benthic oxygen uptake, indicating a moderate impact on benthic remineralization rates at the time of sampling. Our results show considerable effects of ocean warming and sea ice loss on the ecosystem from the surface ocean to the seafloor in the Laptev Sea, which are likely to continue in the coming decades.
Effects of the neurotoxin β-N-methylamino-L-alanine (BMAA) on the early embryonic development of marine shellfish and fish
Authors: Fu Yilei , Li Aifeng , Qiu Jiangbing , Yan Wenhui , Yan Chen , Zhang Lei , Li Min
Published: December 2022

The neurotoxin β-N-methylamino-L-alanine (BMAA) produced by cyanobacteria and diatoms can accumulate in diverse aquatic organisms through the food web. In the present study, embryos of mussel Mytilus galloprovincialis (Lamarck, 1819), oyster Magallana gigas (Thunberg, 1793), and marine medaka Oryzias melastigma (McClelland, 1839) were exposed to BMAA dissolved in seawater and monitored for early developmental effects. Results demonstrated that the embryonic development of mussels and oysters were significantly inhibited when BMAA concentrations were above 100 μg BMAA·HCl/L (0.65 µM) and 800 μg BMAA·HCl/L (5.18 µM), respectively. The shell growth of mussel embryos was also markedly inhibited by BMAA ≥ 100 μg BMAA·HCl/L (0.65 µM). Based on the dose-response curves related to the modified malformation rate of embryos, the median effective concentration (EC50) values of mussel (48 h) and oyster (24 h) embryos were 196 μg BMAA·HCl/L (1.27 µM) and 1660 μg BMAA·HCl/L (10.7 μM), respectively. A sustained and dose-dependent decrease in heart rate was apparent in marine medaka embryos at 9-days post fertilization following BMAA exposure. However, no obvious effect on ATP concentration was noted in these marine medaka embryos. The current study contributes to our understanding of the sublethal effects of BMAA on the early embryonic development of marine bivalves and medaka. Further research examining the long-term effects of BMAA on the early development of marine organisms is necessary to determine seawater quality criteria for protection.
Efficient spatial kelp biomass estimations using acoustic methods
Authors: Kartveit Kyrre Heldal , Filbee-Dexter Karen , Steen Henning , Christensen Lene , Norderhaug Kjell Magnus
Published: December 2022

Kelp forests are the largest vegetated marine ecosystem on earth, but vast areas of their distribution remain unmapped and unmonitored. Efficient and cost-effective methods for measuring the standing biomass of these ecosystems are urgently needed for coastal mapping, ocean accounting and sustainable management of wild harvest. Here we show how widely available acoustic equipment on vessels can be used to perform robust and large-scale (kilometer) quantifications of kelp biomass which can be used in assessments and monitoring programs. We demonstrate how to interpret echograms from acoustic systems into point estimates of standing biomass in order to create spatial maps of biomass distribution. We also explore what environmental conditions are suitable for acoustic measures. This has direct application for blue carbon accounting, coastal monitoring, management of wild seaweed harvest and the protection and conservation of marine habitats supporting high biodiversity.
Reduced salinity exacerbates the viral infection on the coccolithophorid Emiliania huxleyi at elevated pCO2
Authors: Fu Qianqian , Gao Kunshan
Published: December 2022

The blooms of coccolithophores are usually terminated by their viruses. However, little has been documented on the interplay between the host with virus under different environmental conditions. We investigated the relationship of Emiliania huxleyi (BOF92) and its virus (EhV99B1) after the coccolithophorid had acclimated to different levels of salinity (S35: 35‰; S25: 25‰) and CO2 (AC: 400 μatm; HC: 1000 μatm) for about 15 generations. Our results showed that the virus infection decreased the growth of E. huxleyi along with decreased photochemical efficiency and photosynthetic carbon fixation under all the combinations of the treatments. Lowered salinity exacerbated the impacts of the viral infection on the growth and photosynthetic performance of E. huxley. The elevated pCO2 appeared to have alleviated the impacts of the viral infection on its photosynthetic performance with enhanced levels of Fv/Fm, by about 49% under S35 and by about 16% under S25, respectively. Nevertheless, the elevated pCO2 enhanced the virus burst size by about 18% under the high and by about 46% under the low levels of salinity, respectively. It is concluded that reduced salinity under the elevated pCO2 exacerbates the impact of the virus on E. huxleyi, leading to the highest burst sizes and the lowest photosynthetic carbon fixation, and the enhanced photochemical efficiency and increased levels of Chl a under the elevated pCO2 could have facilitated the energy supply for the virus multiplication in the infected cells of E. huxleyi.
Response adaptive mechanisms of three mangrove (Avicennia marina, Aegiceras corniculatum, and Bruguiera gymnorrhiza) plants to waterlogging stress revealed by transcriptome analysis
Authors: Su Bo-Yu , Wang You-Shao , Sun Cui-Ci
Published: December 2022

Distributed in different elevations of the intertidal zone, mangrove plants suffer different periods of flooding, and with varied adaptability to waterlogging from the physiological structure to the metabolic mechanism. Associated with species distribution, transcriptome sequencing was performed to explore the interspecific differences of molecular response mechanisms among Avicennia marina, Aegiceras corniculatum, and Bruguiera gymnorrhiza under waterlogging stress. Results showed that the counts of differentially expressed genes (DEGs) in A. Marina were the highest after stress, followed by Ae. corniculatum and B. gymnorrhiza. It was found that the functions of genes with high differential expression folds (more than eight folds) in the three plants could be classified into four categories: structural regulation, transport, biosynthesis, and protection. It was also found that A. Marina has strong regulation ability in the metabolic process, which can guarantee energy supply and maintain active biosynthesis under waterlogging conditions. In addition, A. Marina was activated in the ethylene synthesis pathway to promote aerenchyma formation and to avoid root tissue hypoxia. Being different from A. Marina and B. gymnorrhiza, Ae. corniculatum down-regulated the XET, SAMS, and ACCO genes, which were involved in the cell wall regulation or ethylene formation that might indicate a different adaptive mechanism. Alcohol dehydrogenase (ADH) and lactate dehydrogenase (LDH) were cloned from A. Marina, Ae. corniculatum, and B. gymnorrhiza. The cloned genes were named as AmADH, AmLDH, AcADH, AcLDH, BgADH, and BgLDH, respectively. qRT-PCR detection verified that LDHs and ADHs were involved in the response of mangrove plants to waterlogging stress, and interspecific difference was observed. The expressions of AcADH and AcLDH were the most prominent. Combined with transcriptome, it was considered that Ae. corniculatum was more dependent on the expression of AcADH and AcLDH that might compensate the weakness of cell wall regulation, whereas A. Marina was more dependent on the regulation of cell structure reversely.
Effect of prehospital transportation on 24-h fluid volume, a post hoc analysis of a multicenter, prospective, observational study on fluid volumes in patients with suspected infection
Authors: Jensen Marie Egebjerg , Kølsen-Petersen Jens Aage , Kirkegaard Hans , Jessen Marie Kristine
Published: December 2022

Introduction

Infections, including sepsis, are leading causes of death and fluid administration is part of the treatment. The optimal fluid therapy remains controversial. If the patient is transported by Emergency Medical Services (EMS), fluids can be initiated during transportation, which may result in increased overall fluid administration and fluid overload, which may be harmful. The aim of the study was to investigate the effect of EMS transportation on 24-h fluid administration in patients with suspected infection.

Methods

This is a post hoc study of a prospective, multicenter, observational study, conducted in three Danish Emergency Departments (EDs), 20 January–2 March 2020, aiming at describing fluid administration in patients with suspected infection. Patients were stratified into the groups: simple infection or sepsis, in accordance with SEPSIS-3-guidelines. The primary outcome of the current study was 24-h total fluid volume (oral and intravenous) stratified by transportation mode to the EDs.

Main results

Total 24-h fluids were registered for 734 patients. Patients with simple infection or sepsis arriving by EMS (n = 388, 54%) received mean 3,774 ml (standard deviation [SD]: 1900) and non-EMS received 3,627 ml (SD: 1568); mean difference (MD) was 303 ml [95% CI: 32; 573] adjusted for age, site, and total SOFA-score. Patients brought in by EMS received more intravenous fluids (MD: 621 ml [95% CI: 378; 864]) and less oral fluids (MD: -474 ml [95% CI: −616; −333]) than non-EMS patients.

Conclusion

Patients transported by EMS received more intravenous fluids and less oral fluids but overall, more fluid in total in the first 24-h than non-EMS after adjusting for age, site and SOFA-score.
The combination of chest compression synchronized ventilation and aortic balloon occlusion improve the outcomes of cardiopulmonary resuscitation in swine
Authors: Xu Jiefeng , Khan Zafar Ullah , Zhang Minhai , Wang Jiangang , Zhou Meiya , Zheng Zhongjun , Chen Qijiang , Zhou Guangju , Zhang Mao
Published: December 2022

Aim

The primary mission of cardiopulmonary resuscitation (CPR) is to provide adequate blood flow and oxygen delivery for restoring spontaneous circulation from cardiac arrest (CA) events. Previously, studies demonstrated that chest compression synchronized ventilation (CCSV) improved systemic oxygen supply during CPR, and aortic balloon occlusion (ABO) augments the efficacy of external CPR by increasing blood perfusion to vital organs. However, both them failed to make a significant improvement in return of spontaneous circulation (ROSC). In this study, we investigated the effects of combined CCSV and ABO on the outcomes of CPR in swine.

Methods

Thirty-one male domestic swine were subjected to 8 min of electrically induced and untreated CA followed by 8 min of CPR. CPR was performed by continuous chest compressions and mechanical ventilation. At the beginning of CPR, the animals were randomized to receive intermittent positive pressure ventilation (IPPV, n = 10), CCSV (n = 7), IPPV + ABO (n = 7), or CCSV + ABO (n = 7). During CPR, gas exchange and systemic hemodynamics were measured, and ROSC was recorded. After resuscitation, the function and injury biomarkers of vital organs including heart, brain, kidney, and intestine were evaluated.

Results

During CPR, PaO2 was significantly higher accompanied by significantly greater regional cerebral oxygen saturation in the CCSV and CCSV + ABO groups than the IPPV group. Coronary perfusion pressure, end-tidal carbon dioxide, and carotid blood flow were significantly increased in the IPPV + ABO and CCSV + ABO groups compared with the IPPV group. ROSC was achieved in five of ten (IPPV), five of seven (CCSV), six of seven (IPPV + ABO), and seven of seven (CCSV + ABO) swine, with the rate of resuscitation success being significantly higher in the CCSV + ABO group than the IPPV group (P = 0.044). After resuscitation, significantly improved myocardial and neurological function, and markedly less cardiac, cerebral, renal, and intestinal injuries were observed in the CCSV + ABO group compared with the IPPV group.

Conclusion

The combination of CCSV and ABO improved both ventilatory and hemodynamic efficacy during CPR, promoted ROSC, and alleviated post-resuscitation multiple organ injury in swine.
High-throughput sequencing reveals omnivorous and preferential diets of the rotifer Polyarthra in situ
Authors: Liang Diwen , Luo Hailin , Huang Chunrong , Ye Zhen , Sun Shuangshuang , Dong Jiahua , Liang Mingyi , Lin Senjie , Yang Yufeng
Published: December 2022

Knowledge of in situ diet of widespread rotifers is crucial for accurately understanding the trophic position, ecological function, and adaptability to environmental changes in aquatic ecosystems. However, it is challenging to achieve the in situ diet information due to the lack of efficient and comprehensive methods. Here, we investigated the diet composition of Polyarthra in a subtropical lake using high-throughput sequencing (HTS) of a rRNA metabarcode for Polyarthra and ambient water samples. After eliminating Polyarthra sequences, a total of 159 operational taxonomic units (OTUs) from taxa in 15 phyla were detected from Polyarthra gut content samples. Most of the OTUs belong to Chlorophyta, followed by unclassified Fungi, Chrysophyta, Dinoflagellata, Ciliophora, Bacillariophyta, Cryptophyta, Arthropoda, Cercozoa, Mollusca, Apicomplexa, Haptophyta, Amoebozoa, Chordata and other eukaryotes. Our results showed that Polyarthra mainly grazed on Chlorophyta, which may result from the high relative abundance of Chlorophyta in ambient waters. In contrast, Chrysophyceae and Synurophyceae were enriched in Polyarthra’s gut, indicating that this rotifer prefers these taxa as food. Moreover, correlation analysis showed that total nitrogen, transparency, depth, Chlorophyll-a and total phosphorus were key factors for the variation of the eukaryotic community in the Polyarthra gut contents. When the concentration of nutrients in the water environment decreased, Polyarthra shifted from herbivorous feeding to more carnivorous feeding. Thus, Polyarthra is generally omnivorous but preference for Chrysophytes and Synurophytes, and it responds to the environmental changes by adopting a flexible feeding strategy. This could partly explain why the widespread rotifers have apparently wide tolerance toward spatial and environmental changes.
Long-term high loading intensity of aerobic exercise improves skeletal muscle performance via the gut microbiota-testosterone axis
Authors: Zhang Lidong , Lang Hedong , Ran Li , Tian Guoliang , Shen Hui , Zhu Jundong , Zhang Qianyong , Yi Long , Mi Mantian
Published: December 2022

Exercise is reported to play a crucial role in skeletal muscle performance. However, the underlying mechanism is still unknown. Thus, we investigated the effect of high-intensity aerobic exercise on skeletal muscle performance. In this study, the male C57BL/6J mice were accepted by high-intensity aerobic exercise for 8 weeks to establish an exercise model. It was observed that high-intensity aerobic exercise markedly affected the expression of genes in skeletal muscle. Moreover, high-intensity aerobic exercise significantly improved skeletal muscle grip strength and serum testosterone levels. HE staining showed that the cross-sectional area (CSA) of the skeletal muscle was successfully increased after 8 weeks of high-intensity aerobic exercise. Additionally, we found that high-intensity aerobic exercise changed gut microbiota structure by altering the abundance of Akkermansia, Allobaculum, and Lactobacillus, which might be related to testosterone production. However, the beneficial effects disappeared after the elimination of the gut microbiota and recovered after fecal microbiota transplantation (FMT) experiments for 1 week. These results indicated that the beneficial effects of high-intensity aerobic exercise on skeletal muscle were partly dependent on the gut microbiota. Our results suggested that long-term high loading intensity of aerobic exercise could improve skeletal muscle performance, which was probably due to the gut microbiota-testosterone axis.
Anti-tuberculosis drug development via targeting the cell envelope of Mycobacterium tuberculosis
Authors: Xu Xinyue , Dong Baoyu , Peng Lijun , Gao Chao , He Zhiqun , Wang Chuan , Zeng Jumei
Published: December 2022

Mycobacterium tuberculosis possesses a dynamic cell envelope, which consists of a peptidoglycan layer, a mycolic acid layer, and an arabinogalactan polysaccharide. This envelope possesses a highly complex and unique structure representing a barrier that protects and assists the growth of M. tuberculosis and allows its adaptation to the host. It regulates the immune response of the host cells, causing their damage. Therefore, the cell envelope of M. tuberculosis is an attractive target for vaccine and drug development. The emergence of multidrug-resistant as well as extensively drug resistant tuberculosis and co-infection with HIV prevented an effective control of this disease. Thus, the discovery and development of new drugs is a major keystone for TB treatment and control. This review mainly summarizes the development of drug enzymes involved in the biosynthesis of the cell wall in M. tuberculosis, and other potential drug targets in this pathway, to provide more effective strategies for the development of new drugs.
5’UTR sequences influence protein levels in Escherichia coli by regulating translation initiation and mRNA stability
Authors: Chen Fan , Cocaign-Bousquet Muriel , Girbal Laurence , Nouaille Sébastien
Published: December 2022

A set of 41 synthetic 5’UTRs with different theoretical translation initiation rates were generated to explore the role of 5’UTRs in the regulation of protein levels in Escherichia coli. The roles of the synthetic 5’UTRs in regulating the expression of different reporter genes were analyzed in vivo. Protein levels varied substantially between the different constructs but for most of the 5’UTRs, protein levels were not correlated with theoretical translation initiation rates. Large variations in mRNA concentrations were measured with the different 5’UTRs even though the same concentration of transcription inducer was used in each case. 5’UTRs were also found to strongly affect mRNA stability, and these changes in mRNA stability often contributed to observed differences in mRNA concentration. Unexpectedly, the effect of the 5’UTRs on mRNA half-lives was found to vary depending on the downstream reporter gene. These results clearly demonstrate that 5’UTRs contribute to gene expression regulation at the level of translation initiation and of mRNA stability, to an extent that depends on the nature of the downstream gene.
Preservation characteristics and bacterial communities of crimped ensiled barley grains modulated by moisture content and additive application
Authors: Franco Marcia , Tapio Ilma , Rinne Marketta
Published: December 2022

Information about the relationships between preservation characteristics and main bacterial communities of fermented feeds can guide decision making during feed preservation and silage additive development. The objective was to evaluate fermentation quality, aerobic stability, microbial quality and bacterial profile of crimped barley grains ensiled under three moisture contents (MC): 228 (low MC), 287 (medium MC) and 345 (high MC) g/kg fresh matter; and using four additive treatments: 1. Control (CONT), 2. Formic and propionic acid-based additive (FPA), 3. Inoculation with homofermentative and heterofermentative strains of lactic acid bacteria (LAB), and 4. Salt-based additive (SALT). There was a quadratic effect (p < 0.05) of incremental MC on pH where greater decline happened from low (5.81) to medium (4.83) MC than from medium to high (4.28) MC, while lactic acid concentration and aerobic stability increased in a linear manner (p < 0.05). Ammonia-N and acetic acid concentrations increased quadratically (p < 0.05) with increasing levels of MC. The effects of additives depended on MC so that improvements in preservation characteristics in response to LAB and SALT were observed at medium and high MC, while FPA was effective at all MC levels. A minor shift was observed in bacterial ecology from raw material towards low MC samples, with Erwiniaceae sp., Enterobacterales spp. and Pseudomonas dominating the fermentation. A major change occurred in medium and high MC materials, where Fructilactobacillus dominated the fermentation in CONT, FPA and SALT silages. LAB-treated silages at medium and high MC resulted in a distinguished pattern with dominance of Lentilactobacillus followed by Lactiplantibacillus. Most abundant communities in the samples, such as Fructilactobacillus, Erwiniaceae sp., Enterobacterales spp. and Pseudomonas, were correlated with several fermentation characteristics. Our results showed that crimped barley grains could be successfully ensiled under various MC and additive treatments. Low MC feeds had higher risk to be aerobically unstable while high MC resulted in more extensive fermentation, with potentially poor fermentation quality. The suitable additive depends on the raw material characteristics as LAB and SALT require relatively high MC to be effective, while FPA showed consistent improvements over all MC levels used in the current study. Awareness of the MC of grain prior to ensiling allows to identify the risks to preservation quality and provides information for choosing an effective additive.
In silico prediction of siRNA ionizable-lipid nanoparticles In vivo efficacy: Machine learning modeling based on formulation and molecular descriptors
Authors: Metwally Abdelkader A. , Nayel Amira A. , Hathout Rania M.
Published: December 2022

In silico prediction of the in vivo efficacy of siRNA ionizable-lipid nanoparticles is desirable as it can save time and resources dedicated to wet-lab experimentation. This study aims to computationally predict siRNA nanoparticles in vivo efficacy. A data set containing 120 entries was prepared by combining molecular descriptors of the ionizable lipids together with two nanoparticles formulation characteristics. Input descriptor combinations were selected by an evolutionary algorithm. Artificial neural networks, support vector machines and partial least squares regression were used for QSAR modeling. Depending on how the data set is split, two training sets and two external validation sets were prepared. Training and validation sets contained 90 and 30 entries respectively. The results showed the successful predictions of validation set log (siRNA dose) with Rval2= 0.86–0.89 and 0.75–80 for validation sets one and two, respectively. Artificial neural networks resulted in the best Rval2 for both validation sets. For predictions that have high bias, improvement of Rval2 from 0.47 to 0.96 was achieved by selecting the training set lipids lying within the applicability domain. In conclusion, in vivo performance of siRNA nanoparticles was successfully predicted by combining cheminformatics with machine learning techniques.
SiMeEx, a simplified method for metabolite extraction of adherent mammalian cells
Authors: Henne Antonia , Vigh Anna , Märtens Andre , Nonnenmacher Yannic , Ohm Melanie , Hosseini Shirin , More Tushar H. , Lauterbach Mario A. , Garritsen Hendrikus , Korte Martin , He Wei , Hiller Karsten
Published: December 2022

A reliable method for metabolite extraction is central to mass spectrometry-based metabolomics. However, existing methods are lengthy, mostly due to the step of scraping cells from cell culture vessels, which restricts metabolomics in broader application such as lower cell numbers and high-throughput studies. Here, we present a simplified metabolite extraction (SiMeEx) method, to efficiently and quickly extract metabolites from adherent mammalian cells. Our method excludes the cell scraping step and therefore allows for a more efficient extraction of polar metabolites in less than 30 min per 12-well plate. We demonstrate that SiMeEx achieves the same metabolite recovery as using a standard method containing a scraping step, in various immortalized and primary cells. Omitting cell scraping does not compromise the performance of non-targeted and targeted GC-MS analysis, but enables metabolome analysis of cell culture on smaller well sizes down to 96-well plates. Therefore, SiMeEx demonstrates advantages not only on time and resources, but also on the applicability in high-throughput studies.
Quality of life of children with residual seizures after epileptic resection surgery
Authors: Wu Yuxin , Zhang Zaiyu , Liang Ping , Zou Bin , Wang Difei , Zhai Xuan
Published: December 2022

Objective

Epilepsy dramatically affects the quality of life (QoL) of children, and resection surgery can improve their QoL by reducing seizures or completely controlling them. Children who have postoperative seizures tend to show a poorer QoL. The aim of the present study was to investigate the QoL of children with seizures after resection surgery and its influencing factors.

Methods

In the present study, we retrospectively reviewed 151 consecutive children who underwent resection surgery. We then divided them into two groups, seizure and seizure-free groups, according to the seizure outcomes 1 year after surgery. Variables were categorized into a number of factor types such as preoperative factors, surgery-related factors, postoperative factors, and family factors. QoL and seizure outcomes more than 3 years after surgery were assessed according to the ILAE seizure outcome classification and the CHEQOL-25 scale.

Results

Forty-three (28.5%) of the 151 children had seizures 1 year after surgery, and two children died during the follow-up period. The mean CHEQOL-25 scale for children with seizures was 63.5 ± 18.2, and 20 (48.8%) patients had poor QoL. Surgery-related factors, such as surgical complications and surgical sequelae, were not statistically associated with QoL. Preoperative language development retardation or language dysfunction [odds ratio (OR) = 29.3, P = 0.012) and postoperative ILAE seizure outcome classification (OR = 1.9, P = 0.045)] were significantly associated with QoL.

Significance

Children with seizures after resection surgery had a relatively poor QoL. Surgery-related factors, such as surgical complications and surgical sequelae, cannot predict the QoL. Preoperative language development retardation or language dysfunction and postoperative ILAE seizure outcome classification were independent predictors of the quality of life (QoL). For children who could not achieve the expected freedom from seizure after surgery, a lower ILAE grade (ILAE 1-3) is also an acceptable outcome since it predicts a higher QoL.
Dynamic cognitive inhibition in the context of frustration: Increasing racial representation of adolescent athletes using mobile community-engaged EEG methods
Authors: Hudac Caitlin M. , Wallace Jessica S. , Ward Victoria R. , Friedman Nicole R. , Delfin Danae , Newman Sharlene D.
Published: December 2022

Introduction

Concussive events and other brain injuries are known to reduce cognitive inhibition, a key aspect of cognition that supports ones' behaviors and impacts regulation of mood or affect. Our primary objective is to investigate how induction of negative affect (such as frustration) impacts cognitive inhibition and the dynamic process by which youth athletes modulate responses. Secondary objective is to address the lack of Black representation in the scientific literature that promotes brain health and investigates pediatric sports-related brain injury. In particular, neuroscience studies predominantly include White participants despite broad racial representation in sport, in part due to technological hurdles and other obstacles that challenge research access for Black participants.

Methods

Using electroencephalography (EEG), we evaluate the dynamic brain processes associated with cognitive inhibition in the context of frustration induction in adolescent athletes during pre-season conditioning (i.e., prior to contact; N = 23) and a subset during post-season (n = 17).

Results

The N2 component was sensitive to frustration induction (decreased N2 amplitude, slower N2 latency), although effects were less robust at postseason. Trial-by-trial changes indicated a steady decrease of the N2 amplitude during the frustration block during the preseason visit, suggesting that affective interference had a dynamic effect on cognitive inhibition. Lastly, exploratory analyses provide preliminary evidence that frustration induction was less effective for athletes with a previous history of concussion or migraines (trending result) yet more effective for athletes endorsing a history with mental health disorders.

Discussion

We emphasize the urgent need to improve representation in cognitive neuroscience, particularly as it pertains to brain health. Importantly, we provide detailed guides to our methodological framework and practical suggestions to improve representative participation in studies utilizing high-density mobile EEG.
Dual fluorescent aptasensor for simultanous and quantitative detection of sulfadimethoxine and oxytetracycin residues in animal-derived foods tissues based on mesoporous silica
Authors: Tang Jiaming , Zheng Xiaoling , Jiang Shuang , Cao Mingdong , Wang Sixian , Zhou Zhaoyang , Nie Xunqing , Fang Yu , Le Tao
Published: December 2022

Herein, we developed a dual fluorescent aptasensor based on mesoporous silica to simultaneously detect sulfadimethoxine (SDM) and oxytetracycline (OTC) in animal-derived foods. We immobilized two types of aptamers modified with FAM and CY5 on the silica surface by base complementary pairing reaction with the cDNA modified with a carboxyl group and finally formed the aptasensor detection platform. Under optimal conditions, the detection range of the aptasensor for SDM and OTC was 3–150 ng/mL (R2 = 0.9831) and 5–220 ng/mL (R2 = 0.9884), respectively. The limits of detection for SDM and OTC were 2.2 and 1.23 ng/mL, respectively. The limits of quantification for SDM and OTC were 7.3 and 4.1 ng/mL, respectively. Additionally, the aptasensor was used to analyze spiked samples. The average recovery rates ranged from 91.75 to 114.65% for SDM and 89.66 to 108.94% for OTC, and all coefficients of variation were below 15%. Finally, the performance and practicability of our aptasensor were confirmed by HPLC, demonstrating good consistency. In summary, this study was the first to use the mesoporous silica-mediated fluorescence aptasensor for simultaneous detection of SDM and OTC, offering a new possibility to analyze other antibiotics, biotoxins, and biomolecules.