bluuebunny/crossref_metadata_2025 huggingface dataset:
Supplementary Material, Table 1, Supplementary Figures 1-8 from Monoallelic IDH1 R132H Mutation Mediates Glioma Cell Response to Anticancer Therapies via Induction of Senescence
Authors: Zhan Daqian , Ma Ding , Wei Shuang , Lal Bachchu , Fu Yi , Eberhart Charles , Laterra John , Ying Mingyao , Li Yunqing , Meeker Alan , Lopez-Bertoni Hernando , Xia Shuli
Published: April 2023

Supplementary Table 1. Primers used for qRT-PCR Supplementary Fig. 1. Heterozygous IDH1R132H/WT enhanced therapy-induced senescence in glioma cells. Supplementary Fig. 2. a. Schematic graph of genome editing strategy with two guide RNAs to knock out human ATRX. b. The ATRX gene sequence of two clones (C4, C10) after genome editing with CRISPR/Cas9 and two ARTX guide RNAs. c. Transwell assays of cell migration showing ATRX knockout in mIDH1 WT cells decreased cell migration. Supplementary Fig. 3. ATRX knockout in SVG IDH1 WT cells did not affect cell growth and migration. Supplementary Fig. 4. a, b. BrdU incorporation assays. c. Cell cycle analysis with PI and flow cytometry. Cells were synchronized by serum withdrawing for 24 h, followed by serum replenishing for 18 h. d. Western blot analyses of Y-H2AX and DNA damage response pathways in cells. Quantification of protein level changes was from the average of three independent experiments. Supplementary Fig. 5. IDH1R132H/WT and ATRX knockout did not induce ALT and alter chromosome numbers in SVG astroglial cells. Supplementary Fig. 6. Effect of AGI-5198 (a) and 5-Aza (b) on SASP expression. Supplementary Fig. 7. M0 human primary macrophages responses to LPS and IL4. Supplementary Fig. 8. ABT-263 decreased SA-beta-gal staining.
Supplemental Figure 5 from Autophagy-Dependent Sensitization of Triple-Negative Breast Cancer Models to Topoisomerase II Poisons by Inhibition of the Nucleosome Remodeling Factor
Authors: Tyutyunyk-Massey Liliya , Sun Yilun , Dao Nga , Ngo Hannah , Dammalapati Mallika , Vaidyanathan Ashish , Singh Manjulata , Haqqani Syed , Haueis Joshua , Finnegan Ryan , Deng Xiaoyan , Kirberger Steve E. , Bos Paula D. , Bandyopadhyay Dipankar , Pomerantz William C.K. , Pommier Yves , Gewirtz David A. , Landry Joseph W.
Published: April 2023

Supplemental Figure 5. Selective BPTF Bromdomain Inhibitor AU1 Recapitulates Effects from BPTF KD in Human MDA-MB-231 and Mouse 66cl4 and 4T07 Cell Lines.
Supplementary Figures 1-12, Supplementary Tables 1-3 from AKT1 E17K Inhibits Cancer Cell Migration by Abrogating β-Catenin Signaling
Authors: Gao Sizhi Paul , Kiliti Amber J. , Zhang Kai , Vasani Naresh , Mao Ninghui , Jordan Emmet , Wise Hannah C. , Shrestha Bhattarai Tripti , Hu Wenhuo , Dorso Madeline , Rodrigues James A. , Kim Kwanghee , Hanrahan Aphrothiti J. , Razavi Pedram , Carver Brett , Chandarlapaty Sarat , Reis-Filho Jorge S. , Taylor Barry S. , Solit David B.
Published: April 2023

S1. Generation of MCF-10A isogenic cells via TP53 knockout and AKT1 E17K knock-in. S2. Frequent co-occurrence of AKT1, TP53 and CDH1 mutations in breast cancers. S3. TP53 CRISPR knockout in MCF-10A cells. S4. Phosphorylation of the AKT1 effector PRAS40 was AKT-dependent in MCF-10A p53ko/E17K cells. S5. Immunofluorescence using a pT308 specific AKT1 antibody of MCF-10A cells following lentiviral infection with V5 tagged (V5 tag) wildtype (WT) or E17K mutant AKT1. S6. Knock-in of the AKT1 E17K mutation did not enhance sensitivity to the pan-AKT kinase inhibitor AZD5363 or the allosteric pan-AKT inhibitor MK2206 in 2Dculture conditions. S7. Survey of previously postulated mechanisms for AKT1-mediated inhibition of cell migration in breast cancer cells. S8. CDH1 promoter methylation status in the MCF-10A isogenic cells. S9. β-catenin interacts with the kinase domain of AKT1. S10. Expression of AKT1 E17K decreased phosphorylation of AKT2 in MCF-10A cells. S11. AKT2 knockdown induces E-cadherin expression in MCF-10A p53ko cells. S12. Model of the mechanism of paradoxical inhibition of cell migration by activated AKT1. Table S1. List of oligonucleotide sequences Table S2. List of antibodies Table S3. List of plasmids
Supplementary Materials and Methods, Table S1, Figures S1-7 from Bidirectional Regulatory Cross-Talk between Cell Context and Genomic Aberrations Shapes Breast Tumorigenesis
Authors: Kumar Brijesh , Bhat-Nakshatri Poornima , Maguire Calli , Jacobsen Max , Temm Constance J. , Sandusky George , Nakshatri Harikrishna
Published: April 2023

Supplementary Table S1: Summary of various isogenic immortalized, transformed, soft agar clones-derived, primary tumor-derived, and metastasis-derived cell lines. Supplementary Figure S1: Characterization of transformed breast epithelial cell lines generated from breast biopsies of healthy European, African, and Hispanic/Latina ancestry women. Supplementary Figure S2: Signaling pathway activation and DNA binding activity in luminal A, luminal-like, normal-like and basal-like transformed cell lines. Supplementary Figure S3: H-RasG12V alone generates tumors at a low frequency. Supplementary Figure S4: Characterization of cell lines derived from tumors of luminal A, luminal-like, normal-like and basal-like double transformed cells. Supplementary Figure S5: Characterization of cell lines derived from soft agar clones. Supplementary Figure S6: Characterization of cell lines derived from paired tumor and metastasis. Supplementary Figure S7: SV40-T/t antigens sensitize cells to doxorubicin.
Supplementary Figure from EML4-ALK Variant 3 Promotes Mitotic Errors and Spindle Assembly Checkpoint Deficiency Leading to Increased Microtubule Poison Sensitivity
Authors: Lucken Kellie , O'Regan Laura , Choi Jene , Sampson Josephina , Pashley Sarah L. , Bayliss Richard , Khan Sam , Fry Andrew M.
Published: April 2023

Supplementary Figure from EML4-ALK Variant 3 Promotes Mitotic Errors and Spindle Assembly Checkpoint Deficiency Leading to Increased Microtubule Poison Sensitivity
Supplementary Figure from circ_0041732 Promotes Breast Cancer Progression
Authors: Ye Guolin , He Suqun , Pan Ruilin , Zhu Lewei , Zhou Dan , Cai Genxi , Chen Peixian
Published: April 2023

Supplementary Figure from circ_0041732 Promotes Breast Cancer Progression
Supplementary Figure from Transcription Factor FOSL1 Enhances Drug Resistance of Breast Cancer through DUSP7-Mediated Dephosphorylation of PEA15
Authors: Li Lin , Wang Nan , Xiong Youyi , Guo Guangcheng , Zhu Mingzhi , Gu Yuanting
Published: April 2023

Supplementary Figure from Transcription Factor FOSL1 Enhances Drug Resistance of Breast Cancer through DUSP7-Mediated Dephosphorylation of PEA15
Supplementary Data from Inactivation of p21-Activated Kinase 2 (Pak2) Inhibits the Development of Nf2-Deficient Tumors by Restricting Downstream Hedgehog and Wnt Signaling
Authors: Sementino Eleonora , Kadariya Yuwaraj , Cheung Mitchell , Menges Craig W. , Tan Yinfei , Kukuyan Anna-Mariya , Shrestha Ujjawal , Karchugina Sofiia , Cai Kathy Q. , Peri Suraj , Duncan James S. , Chernoff Jonathan , Testa Joseph R.
Published: April 2023

Supplementary Data from Inactivation of p21-Activated Kinase 2 (Pak2) Inhibits the Development of Nf2-Deficient Tumors by Restricting Downstream Hedgehog and Wnt Signaling
Supplementary Figure from Small-Molecule NSC59984 Induces Mutant p53 Degradation through a ROS-ERK2-MDM2 Axis in Cancer Cells
Authors: Zhang Shengliang , Zhou Lanlan , El-Deiry Wafik S.
Published: April 2023

Supplementary Figure from Small-Molecule NSC59984 Induces Mutant p53 Degradation through a ROS-ERK2-MDM2 Axis in Cancer Cells
Supplementary Data from In Vitro Insertional Mutagenesis Screen Identifies Novel Genes Driving Breast Cancer Metastasis
Authors: Miskey Csaba , Botezatu Lacramioara , Temiz Nuri A. , Gogol-Döring Andreas , Bartha Áron , Győrffy Balázs , Largaespada David A. , Ivics Zoltán , Sebe Attila
Published: April 2023

Supplementary Data from In Vitro Insertional Mutagenesis Screen Identifies Novel Genes Driving Breast Cancer Metastasis
Supplementary Data from Multi-omics Profiling Shows BAP1 Loss Is Associated with Upregulated Cell Adhesion Molecules in Uveal Melanoma
Authors: Baqai Usman , Purwin Timothy J. , Bechtel Nelisa , Chua Vivian , Han Anna , Hartsough Edward J. , Kuznetsoff Jeffim N. , Harbour J. William , Aplin Andrew E.
Published: April 2023

Supplementary Data from Multi-omics Profiling Shows BAP1 Loss Is Associated with Upregulated Cell Adhesion Molecules in Uveal Melanoma
Supplementary Figure from Intratumoral Electroporation of Plasmid Encoded IL12 and Membrane-Anchored Anti-CD3 Increases Systemic Tumor Immunity
Authors: Han Mia , Nguyen Bianca , Lee Jack Y. , Browning Erica , Zhang Jun , Mukhopadhyay Anandaroop , Gujar Ravindra , Salazar Jon , Hermiz Reneta , Svenson Lauren , Rolig Annah S. , Redmond William L. , Algazi Alain P. , Daud Adil I. , Canton David A. , Twitty Christopher G.
Published: April 2023

Supplementary Figure from Intratumoral Electroporation of Plasmid Encoded IL12 and Membrane-Anchored Anti-CD3 Increases Systemic Tumor Immunity
Supplementary Table from Integrated Proteomics-Based Physical and Functional Mapping of AXL Kinase Signaling Pathways and Inhibitors Define Its Role in Cell Migration
Authors: Majumder Anurima , Hosseinian Sina , Stroud Mia , Adhikari Emma , Saller James J. , Smith Matthew A. , Zhang Guolin , Agarwal Shruti , Creixell Marc , Meyer Benjamin S. , Kinose Fumi , Bowers Kiah , Fang Bin , Stewart Paul A. , Welsh Eric A. , Boyle Theresa A. , Meyer Aaron S. , Koomen John M. , Haura Eric B.
Published: April 2023

Supplementary Table from Integrated Proteomics-Based Physical and Functional Mapping of AXL Kinase Signaling Pathways and Inhibitors Define Its Role in Cell Migration
Supplementary Data from The E3 Ligase MIB1 Promotes Proteasomal Degradation of NRF2 and Sensitizes Lung Cancer Cells to Ferroptosis
Authors: Wang Haiyun , Huang Qiuling , Xia Jianhong , Cheng Shan , Pei Duanqing , Zhang Xiaofei , Shu Xiaodong
Published: April 2023

S1. qRT-PCR analysis in A549. S2. Rescue of H2O2 induced cell death in WT and MIB1 overexpression A549 cells. S3. A549 cells with MIB1 overexpression is more sensitive to RSL3 treatment in mouse xenograft model. S4. Illustration of hMIB1 and hNRF2 mutants used in this study. S5. NRF2 ubiquitination level is reduced in MIB1 knockout MDA-MB-231 cells. Table S1. List of PCR primers used in this study; Table S2. List of antibodies used in this study.
Supplementary Table S4 from Sox2 Is an Oncogenic Driver of Small-Cell Lung Cancer and Promotes the Classic Neuroendocrine Subtype
Authors: Voigt Ellen , Wallenburg Madeline , Wollenzien Hannah , Thompson Ethan , Kumar Kirtana , Feiner Joshua , McNally Moira , Friesen Hunter , Mukherjee Malini , Afeworki Yohannes , Kareta Michael S.
Published: April 2023

Antibodies used in this study
Supplementary Table 1 from XIAP and PHB1 Regulate Anoikis through Competitive Binding to TRAF6
Authors: Yang Bo , Lou Chao , Chen Shengkai , Zhang Zhiyuan , Xu Qin
Published: April 2023

Table S1. The binding sites between TRAF6 and XIAP were predicted by the docking algorithm Cluspro.
Supplementary Table S2 from Sox2 Is an Oncogenic Driver of Small-Cell Lung Cancer and Promotes the Classic Neuroendocrine Subtype
Authors: Voigt Ellen , Wallenburg Madeline , Wollenzien Hannah , Thompson Ethan , Kumar Kirtana , Feiner Joshua , McNally Moira , Friesen Hunter , Mukherjee Malini , Afeworki Yohannes , Kareta Michael S.
Published: April 2023

Tumor scoring in relation to Figure 5F
Supplementary Figure from Loss of PBRM1 Alters Promoter Histone Modifications and Activates ALDH1A1 to Drive Renal Cell Carcinoma
Authors: Schoenfeld David A. , Zhou Royce , Zairis Sakellarios , Su William , Steinbach Nicole , Mathur Deepti , Bansal Ankita , Zachem Alexis L. , Tavarez Bertilia , Hasson Dan , Bernstein Emily , Rabadan Raul , Parsons Ramon
Published: April 2023

Supplementary Figure from Loss of PBRM1 Alters Promoter Histone Modifications and Activates ALDH1A1 to Drive Renal Cell Carcinoma
Supplementary Figure from Diurnal Expression of PD-1 on Tumor-Associated Macrophages Underlies the Dosing Time-Dependent Antitumor Effects of the PD-1/PD-L1 Inhibitor BMS-1 in B16/BL6 Melanoma-Bearing Mice
Authors: Tsuruta Akito , Shiiba Yuki , Matsunaga Naoya , Fujimoto Marina , Yoshida Yuya , Koyanagi Satoru , Ohdo Shigehiro
Published: April 2023

Supplementary Figure from Diurnal Expression of PD-1 on Tumor-Associated Macrophages Underlies the Dosing Time-Dependent Antitumor Effects of the PD-1/PD-L1 Inhibitor BMS-1 in B16/BL6 Melanoma-Bearing Mice
Supplementary Data from Intronic Cis-Element DR8 in hTERT Is Bound by Splicing Factor SF3B4 and Regulates hTERT Splicing in Non–Small Cell Lung Cancer
Authors: Slusher Aaron L. , Kim Jeongjin J. , Ribick Mark , Pollens-Voigt Jesse , Bankhead Armand , Palmbos Phillip L. , Ludlow Andrew T.
Published: April 2023

Supplementary Data from Intronic Cis-Element DR8 in hTERT Is Bound by Splicing Factor SF3B4 and Regulates hTERT Splicing in Non–Small Cell Lung Cancer
Supplementary Figure from BRAFV600E-Driven Lung Adenocarcinoma Requires Copper to Sustain Autophagic Signaling and Processing
Authors: Tsang Tiffany , Gu Xingxing , Davis Caroline I. , Posimo Jessica M. , Miller Zoey A. , Brady Donita C.
Published: April 2023

Supplementary Figure from BRAFV600E-Driven Lung Adenocarcinoma Requires Copper to Sustain Autophagic Signaling and Processing
Supplementary Table from TGF-β-Induced FLRT3 Attenuation Is Essential for Cancer-Associated Fibroblast–Mediated Epithelial–Mesenchymal Transition in Colorectal Cancer
Authors: Yang Mengdi , Li Dan , Jiang Zhiyuan , Li Changcan , Ji Suyuan , Sun Jing , Chang Yujie , Ruan Shunyi , Wang Zhiyu , Liang Rui , Dai Xueyu , Li Bin , Zhao Hui
Published: April 2023

Supplementary Table from TGF-β-Induced FLRT3 Attenuation Is Essential for Cancer-Associated Fibroblast–Mediated Epithelial–Mesenchymal Transition in Colorectal Cancer
Supplementary Fig S2 from Hypertension Promotes the Proliferation and Migration of ccRCC Cells by Downregulation of TIMP3 in Tumor Endothelial Cells through the miR-21–5p/TGFBR2/P38/EGR1 Axis
Authors: Wang Chenguang , Xu Haibo , Liao Xinhui , Wang Weiming , Wu Wanjun , Li Wujiao , Niu Liman , Li Zhichao , Li Aolin , Sun Yangyang , Huang Weiren , Song Fei
Published: April 2023

Supplementary Figure 2 shows the efficiency of endothelial cell depletion in immunomagnetic cell separation, and TIMP3-overexpression in HUVECs suppressed the growth of 786O cancer cells implanted in the subcutaneous tissue of nude mice.
Supplementary Data from RKIP Induction Promotes Tumor Differentiation via SOX2 Degradation in NF2-Deficient Conditions
Authors: Cho Jung-Hyun , Park Soyoung , Kim Soyeong , Kang So-Mi , Woo Tae-Gyun , Yoon Min-Ho , Lee Hyunkee , Jeong Myeonggyo , Park Yeong Hye , Kim Heegyu , Han Young Taek , Suh Young-Ger , Kim Bae-Hoon , Kwon Yonghoon , Yun Hwayoung , Park Bum-Joon
Published: April 2023

Supplementary Data from RKIP Induction Promotes Tumor Differentiation via SOX2 Degradation in NF2-Deficient Conditions
Supplementary Figure from Raptinal Induces Gasdermin E–Dependent Pyroptosis in Naïve and Therapy-Resistant Melanoma
Authors: Vernon Megane , Wilski Nicole A. , Kotas Daniel , Cai Weijia , Pomante Danielle , Tiago Manoela , Alnemri Emad S. , Aplin Andrew E.
Published: April 2023

Supplementary Figure from Raptinal Induces Gasdermin E–Dependent Pyroptosis in Naïve and Therapy-Resistant Melanoma
Supplementary Figures 1-7 from Activation of the JAK/STAT Pathway Leads to BRAF Inhibitor Resistance in BRAFV600E Positive Thyroid Carcinoma
Authors: Limberg Jessica , Egan Caitlin E. , Gray Katherine D. , Singh Mandeep , Loewenstein Zachary , Yang Yanping , Riascos Maria Cristina , Al Asadi Hala , Safe Parima , El Eshaky Steve , Liang Heng , Ullmann Timothy M. , Wang Weibin , Li Wei , Zhang Tuo , Xiang Jenny , Stefanova Dessislava , Jin Moonsoo M. , Zarnegar Rasa , Fahey Thomas J. , Min Irene M.
Published: April 2023

S1. BRAFV600E mutation identified in thyroid cancer cell lines analyzed by RNA-seq.S2. STR analysis in thyroid cancer cell lines used in this study.S3. Gene expression of thyroid-specific genes in thyroid cancer cell lines analyzed by RNA-seq.S4. Dose-dependent toxicity of vemurafenib in BRAFV600E thyroidcancer cells.S5. Vemurafenib dose-dependent curves of vemurafenib-resistantBRAFV600E thyroid cancer cells.S6. Vemurafenib induces apoptosis in 8505C cells.S7. Predicted activators and inhibitors in Vem-resistant BRAF mutant thyroid cancer cells.
Supplementary Figure from cAMP-Mediated Autophagy Promotes Cell Survival via ROS-Induced Activation of PARP1: Implications for Treatment of Acute Lymphoblastic Leukemia
Authors: Richartz Nina , Pietka Wojciech , Gilljam Karin M. , Skah Seham , Skålhegg Bjørn S. , Bhagwat Sampada , Naderi Elin Hallan , Ruud Ellen , Blomhoff Heidi Kiil
Published: April 2023

Supplementary Figure from cAMP-Mediated Autophagy Promotes Cell Survival via ROS-Induced Activation of PARP1: Implications for Treatment of Acute Lymphoblastic Leukemia
Supplementary Figure from Glutamine Metabolism Mediates Sensitivity to Respiratory Complex II Inhibition in Acute Myeloid Leukemia
Authors: Roma Alessia , Tcheng Matthew , Ahmed Nawaz , Walker Sarah , Jayanth Preethi , Minden Mark D. , Hope Kristin , Nekkar Rao Praveen P. , Luc Jessica , Doxey Andrew C. , Reisz Julie A. , Culp-Hill Rachel , D'Alessandro Angelo , Spagnuolo Paul A.
Published: April 2023

Supplementary Figure from Glutamine Metabolism Mediates Sensitivity to Respiratory Complex II Inhibition in Acute Myeloid Leukemia
Supplementary Figure from Dependence on the MUC1-C Oncoprotein in Classic, Variant, and Non–neuroendocrine Small Cell Lung Cancer
Authors: Fushimi Atsushi , Morimoto Yoshihiro , Ishikawa Satoshi , Yamashita Nami , Bhattacharya Atrayee , Daimon Tatsuaki , Rajabi Hasan , Jin Caining , Hagiwara Masayuki , Yasumizu Yota , Luan Zhou , Suo Wenhao , Wong Kwok-Kin , Withers Henry , Liu Song , Long Mark D. , Kufe Donald
Published: April 2023

Supplementary Figure from Dependence on the MUC1-C Oncoprotein in Classic, Variant, and Non–neuroendocrine Small Cell Lung Cancer
SupplementaryFigure from Concurrent Disruption of the Ras/MAPK and NF-κB Pathways Induces Circadian Deregulation and Hepatocarcinogenesis
Authors: Hanley Kaisa L. , Liang Yan , Wang Gaowei , Lin Xiaoxue , Yang Meixiang , Karin Michael , Fu Wenxian , Feng Gen-Sheng
Published: April 2023

SupplementaryFigure from Concurrent Disruption of the Ras/MAPK and NF-κB Pathways Induces Circadian Deregulation and Hepatocarcinogenesis
Supplementary Data from DNA Methyltransferase 3B–Mediated Intratumoral Heterogeneity and Therapeutic Targeting in Breast Cancer Recurrence and Metastasis
Authors: So Jae Young , Yang Howard H. , Park Woo Yong , Skrypek Nicolas , Ishii Hiroki , Chen Jennifer M. , Lee Maxwell P. , Yang Li
Published: April 2023

Supplementary Data from DNA Methyltransferase 3B–Mediated Intratumoral Heterogeneity and Therapeutic Targeting in Breast Cancer Recurrence and Metastasis
Supplementary Data from Concurrent Disruption of the Ras/MAPK and NF-κB Pathways Induces Circadian Deregulation and Hepatocarcinogenesis
Authors: Hanley Kaisa L. , Liang Yan , Wang Gaowei , Lin Xiaoxue , Yang Meixiang , Karin Michael , Fu Wenxian , Feng Gen-Sheng
Published: April 2023

Supplementary Data from Concurrent Disruption of the Ras/MAPK and NF-κB Pathways Induces Circadian Deregulation and Hepatocarcinogenesis
Supplementary Table from Templated Insertions Are Associated Specifically with BRCA2 Deficiency and Overall Survival in Advanced Ovarian Cancer
Authors: Moore Grace , Majumdar Rahul , Powell Simon N. , Khan Atif J. , Weinhold Nils , Yin Shen , Higginson Daniel S.
Published: April 2023

Supplementary Table from Templated Insertions Are Associated Specifically with BRCA2 Deficiency and Overall Survival in Advanced Ovarian Cancer
Supplementary Table from JAZF1: A Metabolic Regulator of Sensitivity to a Polyamine-Targeted Therapy
Authors: Rosario Spencer R. , Jacobi Justine J. , Long Mark D. , Affronti Hayley C. , Rowsam Aryn M. , Smiraglia Dominic J.
Published: April 2023

Supplementary Table from JAZF1: A Metabolic Regulator of Sensitivity to a Polyamine-Targeted Therapy
Supplementary Figure from Molecular Profile Changes in Patients with Castrate-Resistant Prostate Cancer Pre- and Post-Abiraterone/Prednisone Treatment
Authors: Sicotte Hugues , Kalari Krishna R. , Qin Sisi , Dehm Scott M. , Bhargava Vipul , Gormley Michael , Tan Winston , Sinnwell Jason P. , Hillman David W. , Li Ying , Vedell Peter T. , Carlson Rachel E. , Bryce Alan H. , Jimenez Raphael E. , Weinshilboum Richard M. , Kohli Manish , Wang Liewei
Published: April 2023

Supplementary Figure from Molecular Profile Changes in Patients with Castrate-Resistant Prostate Cancer Pre- and Post-Abiraterone/Prednisone Treatment
Supplementary Figure from JAZF1: A Metabolic Regulator of Sensitivity to a Polyamine-Targeted Therapy
Authors: Rosario Spencer R. , Jacobi Justine J. , Long Mark D. , Affronti Hayley C. , Rowsam Aryn M. , Smiraglia Dominic J.
Published: April 2023

Supplementary Figure from JAZF1: A Metabolic Regulator of Sensitivity to a Polyamine-Targeted Therapy
Supplementary Figure from Uveal Melanoma Exosomes Induce a Prometastatic Microenvironment through Macrophage Migration Inhibitory Factor
Authors: Ambrosini Grazia , Rai Alex J. , Carvajal Richard D. , Schwartz Gary K.
Published: April 2023

Supplementary Figure from Uveal Melanoma Exosomes Induce a Prometastatic Microenvironment through Macrophage Migration Inhibitory Factor
Supplementary Figure from Midkine Promotes Metastasis and Therapeutic Resistance via mTOR/RPS6 in Uveal Melanoma
Authors: Karg Margarete M. , John Lukas , Refaian Nasrin , Buettner Christian , Rottmar Tanja , Sommer Jonas , Bock Barbara , Resheq Yazid J. , Ksander Bruce R. , Heindl Ludwig M. , Mackensen Andreas , Bosch Jacobus J.
Published: April 2023

Supplementary Figure from Midkine Promotes Metastasis and Therapeutic Resistance via mTOR/RPS6 in Uveal Melanoma
Supplementary Figure from Redefining CD56 as a Biomarker and Therapeutic Target in Multiple Myeloma
Authors: Cottini Francesca , Rodriguez Jose , Hughes Tiffany , Sharma Nidhi , Guo Ling , Lozanski Gerard , Liu Bei , Cocucci Emanuele , Yang Yiping , Benson Don
Published: April 2023

Supplementary Figure from Redefining CD56 as a Biomarker and Therapeutic Target in Multiple Myeloma
Supplementary Figure from Loss of VOPP1 Contributes to BET Inhibitor Acquired Resistance in Non–Small Cell Lung Cancer Cells
Authors: Sun Lin , Wu Qian , Huan Xia-Juan , Tian Chang-Qing , Wang Ying-Qing , Miao Ze-Hong
Published: April 2023

Supplementary Figure from Loss of VOPP1 Contributes to BET Inhibitor Acquired Resistance in Non–Small Cell Lung Cancer Cells
Supplementary Table from TFAP4 Activates IGF2BP1 and Promotes Progression of Non–Small Cell Lung Cancer by Stabilizing TK1 Expression through m6A Modification
Authors: Shen Qiming , Xu Zhe , Sun Guanghao , Wang Haoyou , Zhang Lin
Published: April 2023

Supplementary Table from TFAP4 Activates IGF2BP1 and Promotes Progression of Non–Small Cell Lung Cancer by Stabilizing TK1 Expression through m6A Modification
Supplementary Data from Reg4 Interacts with CD44 to Regulate Proliferation and Stemness of Colorectal and Pancreatic Cancer Cells
Authors: Bishnupuri Kumar S. , Sainathan Satheesh K. , Ciorba Matthew A. , Houchen Courtney W. , Dieckgraefe Brian K.
Published: April 2023

Supplementary Data from Reg4 Interacts with CD44 to Regulate Proliferation and Stemness of Colorectal and Pancreatic Cancer Cells
Supplementary Figure 3 from DCLK1-Short Splice Variant Promotes Esophageal Squamous Cell Carcinoma Progression via the MAPK/ERK/MMP2 Pathway
Authors: Ge Yang , Fan Xiaona , Huang Xuying , Weygant Nathaniel , Xiao Zeru , Yan Rui , Liu Heshu , Liu Jian , An Guangyu , Yao Jiannan
Published: April 2023

Figure S3 shows DCLK1-L expression in ESCC patients.
Supplementary Figure 2 from DCLK1-Short Splice Variant Promotes Esophageal Squamous Cell Carcinoma Progression via the MAPK/ERK/MMP2 Pathway
Authors: Ge Yang , Fan Xiaona , Huang Xuying , Weygant Nathaniel , Xiao Zeru , Yan Rui , Liu Heshu , Liu Jian , An Guangyu , Yao Jiannan
Published: April 2023

Figure S2 shows DCLK1 protein expression in ESCC tissue and lymph node.
Supplementary Table from Inhibition of LSD1 Attenuates Oral Cancer Development and Promotes Therapeutic Efficacy of Immune Checkpoint Blockade and YAP/TAZ Inhibition
Authors: Alhousami Thabet , Diny Michael , Ali Faiza , Shin Jennifer , Kumar Gaurav , Kumar Vikas , Campbell Joshua D. , Noonan Vikki , Hanna Glenn J. , Denis Gerald V. , Monti Stefano , Kukuruzinska Maria A. , Varelas Xaralabos , Bais Manish V.
Published: April 2023

Supplementary Table from Inhibition of LSD1 Attenuates Oral Cancer Development and Promotes Therapeutic Efficacy of Immune Checkpoint Blockade and YAP/TAZ Inhibition
Supplementary Figure from Inhibition of LSD1 Attenuates Oral Cancer Development and Promotes Therapeutic Efficacy of Immune Checkpoint Blockade and YAP/TAZ Inhibition
Authors: Alhousami Thabet , Diny Michael , Ali Faiza , Shin Jennifer , Kumar Gaurav , Kumar Vikas , Campbell Joshua D. , Noonan Vikki , Hanna Glenn J. , Denis Gerald V. , Monti Stefano , Kukuruzinska Maria A. , Varelas Xaralabos , Bais Manish V.
Published: April 2023

Supplementary Figure from Inhibition of LSD1 Attenuates Oral Cancer Development and Promotes Therapeutic Efficacy of Immune Checkpoint Blockade and YAP/TAZ Inhibition
Supplementary Figure from Targeting Metabolic Adaptations in the Breast Cancer–Liver Metastatic Niche Using Dietary Approaches to Improve Endocrine Therapy Efficacy
Authors: Zuo Qianying , Mogol Ayca Nazli , Liu Yu-Jeh , Santaliz Casiano Ashlie , Chien Christine , Drnevich Jenny , Imir Ozan Berk , Kulkoyluoglu-Cotul Eylem , Park Nicole Hwajin , Shapiro David J. , Park Ben Ho , Ziegler Yvonne , Katzenellenbogen Benita S. , Aranda Evelyn , O'Neill John D. , Raghavendra Akshara Singareeka , Tripathy Debu , Madak Erdogan Zeynep
Published: April 2023

Supplementary Figure from Targeting Metabolic Adaptations in the Breast Cancer–Liver Metastatic Niche Using Dietary Approaches to Improve Endocrine Therapy Efficacy
Supplementary Data from Epigenetic Silencing of 15-Hydroxyprostaglandin Dehydrogenase by Histone Methyltransferase EHMT2/G9a in Cholangiocarcinoma
Authors: Zhang Jinqiang , Chen Weina , Ma Wenbo , Song Kyoungsub , Lee Sean , Han Chang , Wu Tong
Published: April 2023

Supplementary Data from Epigenetic Silencing of 15-Hydroxyprostaglandin Dehydrogenase by Histone Methyltransferase EHMT2/G9a in Cholangiocarcinoma
Supplementary Table 2 from The Spatial Context of Tumor-Infiltrating Immune Cells Associates with Improved Ovarian Cancer Survival
Authors: Steinhart Benjamin , Jordan Kimberly R. , Bapat Jaidev , Post Miriam D. , Brubaker Lindsay W. , Bitler Benjamin G. , Wrobel Julia
Published: April 2023

Table S2. Results from the Cox Proportional Hazards models for two different distance thresholds for B cell and macrophage interaction (20 and 30 microns). Coefficients have been exponentiated and interpreted as hazard ratios. The 'B Cell Macrophage Interaction' variable and age were statistically significant (p <0.05) in both models.
Supplementary Figure 3 from The Spatial Context of Tumor-Infiltrating Immune Cells Associates with Improved Ovarian Cancer Survival
Authors: Steinhart Benjamin , Jordan Kimberly R. , Bapat Jaidev , Post Miriam D. , Brubaker Lindsay W. , Bitler Benjamin G. , Wrobel Julia
Published: April 2023

S3. Kaplan-Meier curves for different combinations of cell distance interactions in tumor regions.
Supplementary Table 1 from Bone Marrow Mesenchymal Stem Cells Induce Metabolic Plasticity in Estrogen Receptor–Positive Breast Cancer
Authors: Buschhaus Johanna M. , Rajendran Shrila , Chen Siyi , Wharram Bryan L. , Bevoor Avinash S. , Cutter Alyssa C. , Humphries Brock A. , Robison Tanner H. , Farfel Alex P. , Luker Gary D.
Published: April 2023

1. Quantified metabolic shifts in response to antiestrogenic therapy and MCT1/4 inhibition. Change in median values in picoseconds of NADH or CFP lifetime (ATP) after addition of each compound. Labels at the top of the table denote the relevant comparison in each column.
Figure S7 from Bone Marrow Mesenchymal Stem Cells Induce Metabolic Plasticity in Estrogen Receptor–Positive Breast Cancer
Authors: Buschhaus Johanna M. , Rajendran Shrila , Chen Siyi , Wharram Bryan L. , Bevoor Avinash S. , Cutter Alyssa C. , Humphries Brock A. , Robison Tanner H. , Farfel Alex P. , Luker Gary D.
Published: April 2023

Figure S7. Inhibition of monocarboxylate transporters regulates intracellular and extracellular lactate. Western blot shows expression of MCT4 in monocultures of MCF7, T47D, HS5, and HS27a cells. Images are from adjacent lanes on one gel and non-adjacent lanes from a second gel. We show β-actin as a loading control. B,C) Graphs show mean values ± standard deviation for relative concentrations of intracellular (B) and extracellular (C) lactate in monocultures of MCF7 cells or co-cultures of MCF7 and HS5 cells treated with 10 µM syrosingopine, an inhibitor of MCT1/4, or vehicle for 3 days. *** p<0.0001 using paired t tests for each comparison.
Figure S5 from Bone Marrow Mesenchymal Stem Cells Induce Metabolic Plasticity in Estrogen Receptor–Positive Breast Cancer
Authors: Buschhaus Johanna M. , Rajendran Shrila , Chen Siyi , Wharram Bryan L. , Bevoor Avinash S. , Cutter Alyssa C. , Humphries Brock A. , Robison Tanner H. , Farfel Alex P. , Luker Gary D.
Published: April 2023

Figure S5. Representative images of glucose and ATP FRET reporters in MSCs. Pseudocolor scale depicts high (red) and low (blue) CFP lifetime. Scale bar = 50 µm. Representative images of intracellular glucose (A) and ATP (B) in HS5 MSCs when in co-culture with ER+ breast cancer cells. Cancer cells are unlabeled in pseudocolor images.
Figure S4 from Bone Marrow Mesenchymal Stem Cells Induce Metabolic Plasticity in Estrogen Receptor–Positive Breast Cancer
Authors: Buschhaus Johanna M. , Rajendran Shrila , Chen Siyi , Wharram Bryan L. , Bevoor Avinash S. , Cutter Alyssa C. , Humphries Brock A. , Robison Tanner H. , Farfel Alex P. , Luker Gary D.
Published: April 2023

Figure S4. Validation of FRET reporters for intracellular glucose and ATP. Pseudocolor scale depicts high (red) and low (blue) CFP lifetime. Scale bar = 50 µm. Symbols show pair-wise comparisons with ****p<0.0001 using one-way ANOVA. A) Representative images and box and whiskers plots of intracellular glucose in MCF7 monocultures with 0-, 5-, or 25-mM glucose. B) Representative images and box and whiskers plots of intracellular ATP in MCF7 monocultures before and after addition of oligomycin.
Figure S3 from Bone Marrow Mesenchymal Stem Cells Induce Metabolic Plasticity in Estrogen Receptor–Positive Breast Cancer
Authors: Buschhaus Johanna M. , Rajendran Shrila , Chen Siyi , Wharram Bryan L. , Bevoor Avinash S. , Cutter Alyssa C. , Humphries Brock A. , Robison Tanner H. , Farfel Alex P. , Luker Gary D.
Published: April 2023

Figure S3. ROS quantification for MSCs in monoculture and co-culture. Flow cytometry data for ROS detected by CellROX Green in HS5 and HS27a MSCs in monoculture or co-culture with MCF7 (A), T47D (B), or HCC1428 (C) ER+ breast cancer cells. Solid and dashed lines depict MSCs in monoculture co-culture, respectively. Cell type of interest is bolded in co-culture comparisons. N>10,000 cells.
Figure S1 from Bone Marrow Mesenchymal Stem Cells Induce Metabolic Plasticity in Estrogen Receptor–Positive Breast Cancer
Authors: Buschhaus Johanna M. , Rajendran Shrila , Chen Siyi , Wharram Bryan L. , Bevoor Avinash S. , Cutter Alyssa C. , Humphries Brock A. , Robison Tanner H. , Farfel Alex P. , Luker Gary D.
Published: April 2023

Figure S1. Representative images of NADH lifetimes for various experimental conditions. Cell type of interest is bolded in co-culture comparisons. Top row of images denotes cancer cells marked with nuclear mCherry. Pseudocolor scale depicts high (red) and low (blue) NADH lifetime. Scale bar = 50 µm. A) HS5 and HS27a MSCs in monoculture. B-C) MCF7 (B) or T47D (C) cells in monoculture or with conditioned media (CM) from listed MSCs. D) HCC1428 (HCC) cancer cells in monoculture, CM from MSCs, or direct co-culture with MSCs.
Supplementary Table 4 from Improved Delineation of Colorectal Cancer Molecular Subtypes and Functional Profiles with a 62-Gene Panel
Authors: Bhukdee Dhup , Nuwongsri Pattarin , Israsena Nipan , Sriswasdi Sira
Published: April 2023

Gene members of sub-pathway modules
Supplementary Figures 1-16 from Improved Delineation of Colorectal Cancer Molecular Subtypes and Functional Profiles with a 62-Gene Panel
Authors: Bhukdee Dhup , Nuwongsri Pattarin , Israsena Nipan , Sriswasdi Sira
Published: April 2023

S1. Expression profiles of cell type-specific markers.
S2. Example of a subnetwork consisting of 1000 samples from the original CMS dataset.
S3. Robustness analysis of the 2-round network clustering method.
S4. Hierarchical clustering of CMS2 samples.
S5. Hierarchical clustering of CMS4 samples.
S6. Agreement between original CMS and new intra-CMS subtypes.
S7. Comparison of our gene panel with previously published panels for CRC.
S8. Expression of 62-gene panel on CMS2/iCMS2 samples in the SG-Bulk cohort.
S9. Expression of 62-gene panel on every epithelial cell from four selected patients from the iCMS cohort.
S10. Progressive changes in inflammatory, immune, and proliferative pathway activities from CMS2 to CMS4 intra-subtypes.
S11. Expression profiles of cellular senescence markers.
S12. Mutation profiles and rates for KRAS, BRAF and PIK3CA.
S13. Progressive changes in inflammatory, immune, and proliferative pathway activities between CMS1, CMS3, and CMS4 intra-subtypes.
S14. Progressive changes in WNT, EMT, MAPK, and VEGF sub-pathway modules.
S15. Expression profiles for JAK-STAT and amino sugar and nucleotide sugar metabolism pathways.
S16. Confusion matrix for the classification of 7 intra-subtypes with an artificial neural network model.
Figure S1 from The Elongin BC Complex Negatively Regulates AXL and Marks a Differentiated Phenotype in Melanoma
Authors: Schieven Sebastiaan M. , Traets Joleen J.H. , Vliet Alex v. , Baalen Martijn v. , Song Ji-Ying , Guimaraes Marcos Da Silva , Kuilman Thomas , Peeper Daniel S.
Published: April 2023

Extended data on Figure 1: Whole genome-wide CRISPR-Cas9 screenidentifies several negative regulators of AXL.
Supplementary Figures S1-S9 and Tables S1-S4. from MUC1-C Dictates PBRM1-Mediated Chronic Induction of Interferon Signaling, DNA Damage Resistance, and Immunosuppression in Triple-Negative Breast Cancer
Authors: Yamashita Nami , Morimoto Yoshihiro , Fushimi Atsushi , Ahmad Rehan , Bhattacharya Atrayee , Daimon Tatsuaki , Haratake Naoki , Inoue Yuka , Ishikawa Satoshi , Yamamoto Masaaki , Hata Tsuyoshi , Akiyoshi Sayuri , Hu Qiang , Liu Tao , Withers Henry , Liu Song , Shapiro Geoffrey I. , Yoshizumi Tomoharu , Long Mark D. , Kufe Donald
Published: April 2023

S1. Silencing MUC1-C downregulates IRF1 expression in TNBC cells.
S2. Effects of silencing MUC1-C on TNBC cells.
S3. MUC1-C is necessary for PBRM1 expression A and B.
S4. MUC1-C forms a direct complex with IRF1 that activates type I and II ISGs.
S5. Common genes regulated in cells silenced for MUC1, IRF1, and PBRM1.
S6. Silencing MUC1-C, IRF1, and PBRM1 downregulated IDO1 and WARS in TNBC cells.
S7. Silencing of MUC1-C, IRF1, and PBRM1 downregulates RIG-I and MDA5 in TNBC cells.
S8. Silencing MUC1-C, IRF1, and PBRM1 downregulates ISG15 expression in TNBC cells.
S9. Effects of silencing IRF1 and PBRM1 on BT-549 mammosphere formation and GO-203 treatment on the viability of olaparib-resistant MDA-MB-436RR cells.
Table S1. Primers used for qRT-PCR.
Table S2. Primers used for ChIP-qPCR and DNase I chromatin accessibility assays.
Table S3. Common downregulated 196 DEGs in BT-549 cells with silencing of MUC1, IRF1, and PBRM1.
Table S4. Overlapping IFN-pathway genes downregulated in cells with MUC1-C, PBRM1, and IRF1 silencing.
Supplementary Data Table S1 from JMJD6 Shapes a Pro-tumor Microenvironment via ANXA1-Dependent Macrophage Polarization in Breast Cancer
Authors: Cioni Bianca , Ratti Silvia , Piva Annamaria , Tripodi Irene , Milani Matteo , Menichetti Francesca , Langella Tiziana , Botti Laura , De Cecco Loris , Chiodoni Claudia , Lecis Daniele , Colombo Mario P.
Published: April 2023

List of BC patients with ER-positive tumors included in the METABRIC dataset
Figure S6 from Scaffolding Protein Connector Enhancer of Kinase Suppressor of Ras 1 (CNKSR1) Regulates MAPK Inhibition Responsiveness in Pancreas Cancer via Crosstalk with AKT Signaling
Authors: Li Dandan , Miermont Anne M. , Sable Rushikesh , Quadri Humair S. , Mathews Griner Lesley A. , Martin Scott E. , Odzorig Taivan , De Soumita , Ferrer Marc , Powers Astin S. , Hewitt Stephen M. , Rudloff Udo
Published: April 2023

MEK blockade fails to disrupt protein-protein interactions of CNKSR1, KSR1/2, and MEK in CNKSR1low cancer cells.
Figure S5 from Scaffolding Protein Connector Enhancer of Kinase Suppressor of Ras 1 (CNKSR1) Regulates MAPK Inhibition Responsiveness in Pancreas Cancer via Crosstalk with AKT Signaling
Authors: Li Dandan , Miermont Anne M. , Sable Rushikesh , Quadri Humair S. , Mathews Griner Lesley A. , Martin Scott E. , Odzorig Taivan , De Soumita , Ferrer Marc , Powers Astin S. , Hewitt Stephen M. , Rudloff Udo
Published: April 2023

Selumetinib fails to recruit CNKSR1 and p-AKT to the plasma membrane in CNKSR1 low cell lines; Proximal ligation of CNKSR1 and p-AKT in CNKSR1 low cell lines treated with vehicle vs selumetinib;CNKSR1 p-AKT PLA signal after treatment with selumetinib is dependent on the presence of both primary antibodies; Silencing of CNKSR1 in the CNKSR1high cell lines abrogates PLA signal; and Immunofluorescence staining of PIP3 and CNKSR1 in YAPC treated with vehicle and selumetinib.
Figure S2 from Scaffolding Protein Connector Enhancer of Kinase Suppressor of Ras 1 (CNKSR1) Regulates MAPK Inhibition Responsiveness in Pancreas Cancer via Crosstalk with AKT Signaling
Authors: Li Dandan , Miermont Anne M. , Sable Rushikesh , Quadri Humair S. , Mathews Griner Lesley A. , Martin Scott E. , Odzorig Taivan , De Soumita , Ferrer Marc , Powers Astin S. , Hewitt Stephen M. , Rudloff Udo
Published: April 2023

Secondary validation of candidate genes.
Figure S1 from ADAM Metallopeptidase Domain 12 Facilitates Colorectal Cancer Progression by Inhibiting Hippo Signaling Pathway Activity
Authors: Wang XiaoPeng , Zhu Mo , Zuo Hao , Hou Guowei , Xie Rui
Published: April 2023

Figure S1. The correlation analysis between ADAM12 and YAP1. (A) The correlation analysis between ADAM12 and YAP1 in CRC based on the TCGA-COAD data. (B) The correlation analysis between ADAM12 and YAP1 in CRC patients. (C) CRC patients with high YAP1 expression showed markedly lower 2000-days survival than those with low YAP1 expression. (D) Western blot analysis of ADAM12 in T84 cells with YAP1 knockdown.
Data from Energy Management by Enhanced Glycolysis in G1-phase in Human Colon Cancer Cells In Vitro and In Vivo
Authors: Bao Yan , Mukai Kuniaki , Hishiki Takako , Kubo Akiko , Ohmura Mitsuyo , Sugiura Yuki , Matsuura Tomomi , Nagahata Yoshiko , Hayakawa Noriyo , Yamamoto Takehiro , Fukuda Ryo , Saya Hideyuki , Suematsu Makoto , Minamishima Yoji Andrew
Published: April 2023

Abstract
Activation of aerobic glycolysis in cancer cells is well known as the Warburg effect, although its relation to cell- cycle progression remains unknown. In this study, human colon cancer cells were labeled with a cell-cycle phase-dependent fluorescent marker Fucci to distinguish cells in G1-phase and those in S + G2/M phases. Fucci-labeled cells served as splenic xenograft transplants in super-immunodeficient NOG mice and exhibited multiple metastases in the livers, frozen sections of which were analyzed by semiquantitative microscopic imaging mass spectrometry. Results showed that cells in G1-phase exhibited higher concentrations of ATP, NADH, and UDP-N-acetylglucosamine than those in S and G2–M phases, suggesting accelerated glycolysis in G1-phase cells in vivo. Quantitative determination of metabolites in cells synchronized in S, G2–M, and G1 phases suggested that efflux of lactate was elevated significantly in G1-phase. By contrast, ATP production in G2–M was highly dependent on mitochondrial respiration, whereas cells in S-phase mostly exhibited an intermediary energy metabolism between G1 and G2–M phases. Isogenic cells carrying a p53-null mutation appeared more active in glycolysis throughout the cell cycle than wild-type cells. Thus, as the cell cycle progressed from G2–M to G1 phases, the dependency of energy production on glycolysis was increased while the mitochondrial energy production was reciprocally decreased.

Implications: These results shed light on distinct features of the phase-specific phenotypes of metabolic systems in cancer cells. Mol Cancer Res; 11(9); 973–85. ©2013 AACR.
Data from RAS/MEK–Independent Gene Expression Reveals BMP2-Related Malignant Phenotypes in the Nf1-Deficient MPNST
Authors: Sun Daochun , Haddad Ramsi , Kraniak Janice M. , Horne Steven D. , Tainsky Michael A.
Published: April 2023

Abstract
Malignant peripheral nerve sheath tumor (MPNST) is a type of soft tissue sarcoma that occurs in carriers of germline mutations in Nf1 gene as well as sporadically. Neurofibromin, encoded by the Nf1 gene, functions as a GTPase-activating protein (GAP) whose mutation leads to activation of wt-RAS and mitogen-activated protein kinase (MAPK) signaling in neurofibromatosis type I (NF1) patients' tumors. However, therapeutic targeting of RAS and MAPK have had limited success in this disease. In this study, we modulated NRAS, mitogen-activated protein/extracellular signal–regulated kinase (MEK)1/2, and neurofibromin levels in MPNST cells and determined gene expression changes to evaluate the regulation of signaling pathways in MPNST cells. Gene expression changes due to neurofibromin modulation but independent of NRAS and MEK1/2 regulation in MPNST cells indicated bone morphogenetic protein 2 (Bmp2) signaling as a key pathway. The BMP2-SMAD1/5/8 pathway was activated in NF1-associated MPNST cells and inhibition of BMP2 signaling by LDN-193189 or short hairpin RNA (shRNA) to BMP2 decreased the motility and invasion of NF1-associated MPNST cells. The pathway-specific gene changes provide a greater understanding of the complex role of neurofibromin in MPNST pathology and novel targets for drug discovery. Mol Cancer Res; 11(6); 616–27. ©2013 AACR.
Data from Interleukin-6 and Oncostatin-M Synergize with the PI3K/AKT Pathway to Promote Aggressive Prostate Malignancy in Mouse and Human Tissues
Authors: Smith Daniel A. , Kiba Atsushi , Zong Yang , Witte Owen N.
Published: April 2023

Abstract
Chronic inflammation has been proposed as an etiological and progression factor in prostate cancer. In this study, we used a dissociated prostate tissue recombination system to interrogate the role of interleukin 6 (IL6) and the related cytokine oncostatin-M (OSM) in the initiation and progression of prostate cancer. We identified that prostatic intraepithelial neoplasia (PIN) lesions induced by PTEN loss of function (PTENLOF) progress to invasive adenocarcinoma following paracrine expression of either cytokine. Increased expression of OSM was also able to drive progression of benign human epithelium when combined with constitutively activated AKT. Malignant progression in the mouse was associated with invasion into the surrounding mesenchyme and increased activation of STAT3 in PTENLOF grafts expressing IL6 or OSM. Collectively, our work indicates that pro-inflammatory cytokines such as IL6 or OSM could activate pathways associated with prostate cancer progression and synergize with cell-autonomous oncogenic events to promote aggressive malignancy.

Implications: Increased expression of IL6 or OSM synergizes with loss of PTEN to promote invasive prostate cancer.

Visual Overview: http://mcr.aacrjournals.org/content/early/2013/09/02/1541-7786.MCR-13-0238/F1.large.jpg. Mol Cancer Res; 11(10); 1159–65. ©2013 AACR.
Data from Crizotinib-Resistant NPM-ALK Mutants Confer Differential Sensitivity to Unrelated Alk Inhibitors
Authors: Ceccon Monica , Mologni Luca , Bisson William , Scapozza Leonardo , Gambacorti-Passerini Carlo
Published: April 2023

Abstract
The dual ALK/MET inhibitor crizotinib was recently approved for the treatment of metastatic and late-stage ALK+ NSCLC, and is currently in clinical trial for other ALK-related diseases. As predicted after other tyrosine kinase inhibitors' clinical experience, the first mutations that confer resistance to crizotinib have been described in patients with non–small cell lung cancer (NSCLC) and in one patient inflammatory myofibroblastic tumor (IMT). Here, we focused our attention on the anaplastic large cell lymphoma (ALCL), where the oncogenic fusion protein NPM-ALK, responsible for 70% to 80% of cases, represents an ideal crizotinib target. We selected and characterized 2 human NPM-ALK+ ALCL cell lines, KARPAS-299 and SUP-M2, able to survive and proliferate at different crizotinib concentrations. Sequencing of ALK kinase domain revealed that a single mutation became predominant at high crizotinib doses in each cell line, namely L1196Q and I1171N in Karpas-299 and SUP-M2 cells, respectively. These mutations also conferred resistance to crizotinib in Ba/F3 cells expressing human NPM-ALK. The resistant cell populations, as well as mutated Ba/F3 cells, were characterized for sensitivity to two additional ALK inhibitors: the dual ALK/EGFR inhibitor AP26113 and NVP-TAE684. While L1196Q-positive cell lines were sensitive to both inhibitors, cells carrying I1171N substitution showed cross-resistance to all ALK inhibitors tested. This study provides potentially relevant information for the management of patients with ALCL that may relapse after crizotinib treatment. Mol Cancer Res; 11(2); 122–32. ©2012 AACR.
Data from AKR1B10, a Transcriptional Target of p53, Is Downregulated in Colorectal Cancers Associated with Poor Prognosis
Authors: Ohashi Tomoko , Idogawa Masashi , Sasaki Yasushi , Suzuki Hiromu , Tokino Takashi
Published: April 2023

Abstract
p53 is one of the most important tumor suppressor genes, and it is frequently inactivated in various cancers. p53 modulates various cellular functions, such as apoptosis and cell-cycle arrest via transcriptional regulation. Recently, p53 has been reported to be involved in a wide range of cellular metabolic pathways, including glycolysis, oxidative phosphorylation, glutaminolysis, and the antioxidant response. To understand the functional mechanism of p53, it is important to find out the direct transcriptional targets of p53. In this study, aldo-keto reductase family 1, member B10 (AKR1B10) was identified as a direct target of the p53 family by cDNA microarray analysis after comparing the mRNA expression of control and H1299 cells that overexpressed with p53 family members. In addition, we found that the expression of AKR1B10 was significantly decreased in colorectal cancers and adenomas as compared with normal colon tissues. Knockdown of AKR1B10 significantly inhibited p53-induced apoptosis in colorectal cancer cells, whereas the overexpression of AKR1B10 enhanced p53-induced apoptosis and inhibited tumor proliferation in vivo. Furthermore, low expression of AKR1B10 in colon cancer patients was correlated with decreased survival and poor prognosis. These results suggest that decreased expression of AKR1B10 could disrupt the tumor suppressive function of p53, which result in decreased survival in colorectal cancer patients. In summary, AKR1B10 may be a novel prognostic predictor and a novel therapeutic target for colorectal cancer.

Implications: AKR1B10, a transcriptional target of p53, is also a novel prognostic and therapeutic molecule in colorectal cancer. Mol Cancer Res; 11(12); 1554–63. ©2013 AACR.