bluuebunny/crossref_metadata_2025 huggingface dataset:
Data from BAFF Attenuates Immunosuppressive Monocytes in the Melanoma Tumor Microenvironment
Authors: Liu Wei , Stachura Paweł , Xu Haifeng C. , Váraljai Renáta , Shinde Prashant , Ganesh Nikkitha Umesh , Mack Matthias , Van Lierop Anke , Huang Anfei , Sundaram Balamurugan , Lang Karl S. , Picard Daniel , Fischer Ute , Remke Marc , Homey Bernhard , Roesch Alexander , Häussinger Dieter , Lang Philipp A. , Borkhardt Arndt , Pandyra Aleksandra A.
Published: April 2023

Abstract
Emerging evidence indicates B-cell activating factor (BAFF, Tnfsf13b) to be an important cytokine for antitumor immunity. In this study, we generated a BAFF-overexpressing B16.F10 melanoma cell model and found that BAFF-expressing tumors grow more slowly in vivo than control tumors. The tumor microenvironment (TME) of BAFF-overexpressing tumors had decreased myeloid infiltrates with lower PD-L1 expression. Monocyte depletion and anti-PD-L1 antibody treatment confirmed the functional importance of monocytes for the phenotype of BAFF-mediated tumor growth delay. RNA sequencing analysis confirmed that monocytes isolated from BAFF-overexpressing tumors were characterized by a less exhaustive phenotype and were enriched for in genes involved in activating adaptive immune responses and NF-κB signaling. Evaluation of patients with late-stage metastatic melanoma treated with inhibitors of the PD-1/PD-L1 axis demonstrated a stratification of patients with high and low BAFF plasma levels. Patients with high BAFF levels experienced lower responses to anti-PD-1 immunotherapies. In summary, these results show that BAFF, through its effect on tumor-infiltrating monocytes, not only impacts primary tumor growth but can serve as a biomarker to predict response to anti-PD-1 immunotherapy in advanced disease.
Significance:
The BAFF cytokine regulates monocytes in the melanoma microenvironment to suppress tumor growth, highlighting the importance of BAFF in antitumor immunity.
Data from ANGPTL4-Mediated Promotion of Glycolysis Facilitates the Colonization of Fusobacterium nucleatum in Colorectal Cancer
Authors: Zheng Xin , Liu Rui , Zhou Chenchen , Yu Haopeng , Luo Wanyi , Zhu Jianhui , Liu Jiaxin , Zhang Zhe , Xie Na , Peng Xian , Xu Xin , Cheng Lei , Yuan Quan , Huang Canhua , Zhou Xuedong
Published: April 2023

Abstract
Colorectal cancer is a severe health problem worldwide, and accumulating evidence supports the contribution of Fusobacterium nucleatum (F. nucleatum) to colorectal cancer development, metastasis, and chemoresistance. However, the mechanisms underlying the colonization of F. nucleatum in colorectal cancer tissue are not yet clarified. Here we demonstrate that F. nucleatum infection mediated elevation of angiopoietin-like 4 (ANGPTL4) expression. Upregulated ANGPTL4 promoted glucose uptake and glycolysis activity in colorectal cancer cells in vitro and in vivo, which are necessary for the colonization of F. nucleatum. Furthermore, overall increased acetylation of histone H3 lysine 27 was observed in F. nucleatum–infected colorectal cancer cells and patient tumors, which was responsible for the corresponding transcriptional upregulation of ANGPTL4. These data indicate that the metabolic reprogramming of cancer cells induced by F. nucleatum is essential for its enrichment and persistence in colorectal cancer, providing a novel potential target for the clinical intervention of F. nucleatum–related colorectal cancer.
Significance:
F. nucleatum colonization in colorectal cancer is regulated by ANGPTL4-mediated glycolysis, suggesting that this axis could be targeted for combined repression of F. nucleatum and cancer progression.
Supplementary Movie 1 from Changes in Aged Fibroblast Lipid Metabolism Induce Age-Dependent Melanoma Cell Resistance to Targeted Therapy via the Fatty Acid Transporter FATP2
Authors: Alicea Gretchen M. , Rebecca Vito W. , Goldman Aaron R. , Fane Mitchell E. , Douglass Stephen M. , Behera Reeti , Webster Marie R. , Kugel Curtis H. , Ecker Brett L. , Caino M. Cecilia , Kossenkov Andrew V. , Tang Hsin-Yao , Frederick Dennie T. , Flaherty Keith T. , Xu Xiaowei , Liu Qin , Gabrilovich Dmitry I. , Herlyn Meenhard , Blair Ian A. , Schug Zachary T. , Speicher David W. , Weeraratna Ashani T.
Published: April 2023

Young BODIPY-C12 fibroblasts with GFP-Tagged melanoma cells showing lipid uptake
Data from Changes in Aged Fibroblast Lipid Metabolism Induce Age-Dependent Melanoma Cell Resistance to Targeted Therapy via the Fatty Acid Transporter FATP2
Authors: Alicea Gretchen M. , Rebecca Vito W. , Goldman Aaron R. , Fane Mitchell E. , Douglass Stephen M. , Behera Reeti , Webster Marie R. , Kugel Curtis H. , Ecker Brett L. , Caino M. Cecilia , Kossenkov Andrew V. , Tang Hsin-Yao , Frederick Dennie T. , Flaherty Keith T. , Xu Xiaowei , Liu Qin , Gabrilovich Dmitry I. , Herlyn Meenhard , Blair Ian A. , Schug Zachary T. , Speicher David W. , Weeraratna Ashani T.
Published: April 2023

Older patients with melanoma (>50 years old) have poorer prognoses and response rates to targeted therapy compared with young patients (<50 years old), which can be driven, in part, by the aged microenvironment. Here, we show that aged dermal fibroblasts increase the secretion of neutral lipids, especially ceramides. When melanoma cells are exposed to the aged fibroblast lipid secretome, or cocultured with aged fibroblasts, they increase the uptake of lipids via the fatty acid transporter FATP2, which is upregulated in melanoma cells in the aged microenvironment and known to play roles in lipid synthesis and accumulation. We show that blocking FATP2 in melanoma cells in an aged microenvironment inhibits their accumulation of lipids and disrupts their mitochondrial metabolism. Inhibiting FATP2 overcomes age-related resistance to BRAF/MEK inhibition in animal models, ablates tumor relapse, and significantly extends survival time in older animals.
Significance:
These data show that melanoma cells take up lipids from aged fibroblasts, via FATP2, and use them to resist targeted therapy. The response to targeted therapy is altered in aged individuals because of the influences of the aged microenvironment, and these data suggest FATP2 as a target to overcome resistance.

See related commentary by Montal and White, p. 1255..

This article is highlighted in the In This Issue feature, p. 1241
Supplementary Figures S19-S22 from Ultra High-Plex Spatial Proteogenomic Investigation of Giant Cell Glioblastoma Multiforme Immune Infiltrates Reveals Distinct Protein and RNA Expression Profiles
Authors: Bonnett Shilah A , Rosenbloom Alyssa B , Ong Giang T , Conner Mark , Rininger Aric BE , Newhouse Daniel , New Felicia , Phan Chi Q. , Ilcisin Saskia , Sato Hiromi , Lyssand John S , Geiss Gary , Beechem Joseph M.
Published: April 2023

Supplementary Figure S19: Representative images used in the assessment of spatial proteogenomic data quality on Glioblastoma multiforme Grade 4. (A) Human brain glioblastoma tissue array was stained with the GeoMx NGS Human Protein modules (147-plex), WTA, and antibodies against GFAP (Astrocyte/Tumor; left panel), CD45 (Immune; middle panel) and Syto13 (Nuclei; right panel). (B) ROI were segmented into GFAP+ (left panel), CD45+ (middle panel) or Syto13 (right panel).
Supplementary Figure S20: Representative images used in the assessment of spatial proteogenomic data quality on gcGBM and GBM. ROIs for gcGBM (top panel) and GBM (bottom panel) were segmented into GFAP+ (Astrocyte/Tumor; left panel), CD45+ (Immune; middle panel) and Syto13 (Nuclear, DNA; right panel).

Supplementary Figure S21: Combined volcano plot resulting from the differential expression analysis between GBM and gcGBM for all AOIs within the left frontal lobe for RNA (●) and protein (♦) analytes. Targets with significantly differential expression are highlighted either orange (P-value < 0.05), green (FDR < 0.05) or blue (FDR < 0.01); whereas targets in grey show no significant difference in expression. Genes with significantly differential expression are highlighted either orange (P-value <0.05), green (FDR < 0.05) or blue (FDR <0.01); whereas genes in grey show no significant difference in expression. A subset of differentially expressed genes are labeled.

Supplementary Figure S22: Differential protein expression analysis between GBM and gcGBM. Volcano showing the differential protein expression profiles for (A) CD45- and (B) GFAP-enriched segments within the left frontal lobe. Proteins with significantly differential expression are highlighted either orange (P-value <0.05), green (FDR < 0.05) or blue (FDR < 0.01), whereas proteins in grey show no significant difference in expression. A subset of differentially expressed proteins are labeled.
Supplemental Table 1 from Treatment Patterns and Outcomes of Preoperative Neoadjuvant Radiotherapy in Patients with Early-onset Rectal Cancer
Authors: Badesha Jasleen K. , Zhou Marilyn , Arif Arif A. , Gill Sharlene , Speers Caroline , Peacock Michael , De Vera Mary A. , Stuart Heather C. , Brown Carl J. , Donellan Fergal , Loree Jonathan M.
Published: April 2023

Supplemental Table 1 – Characteristics and treatment response in rectal cancers treated with neoadjuvant therapy.
Data from Treatment Patterns and Outcomes of Preoperative Neoadjuvant Radiotherapy in Patients with Early-onset Rectal Cancer
Authors: Badesha Jasleen K. , Zhou Marilyn , Arif Arif A. , Gill Sharlene , Speers Caroline , Peacock Michael , De Vera Mary A. , Stuart Heather C. , Brown Carl J. , Donellan Fergal , Loree Jonathan M.
Published: April 2023

Preoperative radiotherapy for early-stage rectal cancer has risks and benefits that may impact treatment choice in young patients. We reviewed radiotherapy use and outcomes for rectal cancer by age. Patients with early-stage rectal cancer in the Canadian province of British Columbia from 2002 to 2016 were identified (n = 6,232). Baseline characteristics, treatment response, overall survival (OS), disease-free survival (DFS), disease-specific survival (DSS), and locoregional recurrence rate (LRR) were compared between patients <50 (early-onset; n = 532) and ≥50 years old (average-onset; n = 5,700). Early-onset patients were more likely to receive preoperative chemoradiotherapy than short-course radiotherapy [OR, 2.20; 95% confidence interval (CI), 1.67–2.89; P < 0.0001], but also had higher nodal (P = 0.00096) and overall clinical staging (P = 0.033). Cancer downstaging and pathologic complete response rates were similar in those receiving neoadjuvant chemoradiotherapy by age. Early-onset and average-onset patients had similar DSS (P = 0.91) and DFS (P = 0.27) in multivariate analysis unless non-colorectal deaths, which were higher in older patients, were censored in the DFS model (HR, 1.30; 95% CI, 1.01–1.68; P = 0.042). LRR also did not differ between age groups (P = 0.88). Outcomes did not differ based on radiotherapy type. Young patients with rectal cancer are more likely to present with higher staging and receive long-course chemoradiotherapy. DSS did not differ by age group; however, young patients had worse DFS when we censored competing risks of death in older patients.
Significance:
This population-based study suggests younger patients are more likely to receive chemoradiotherapy, potentially due to higher stage at diagnosis, and response is comparable by age.
Ideação suicida em pessoas com transtornos relacionados a substâncias em tratamento
Authors: Vale Caio Cézar , Zerwes Aline Cristina , Capistrano Fernanda Carolina , Kaled Manuela , Maftum Mariluci , Palm Rosibeth
Published: April 2023

Objetivo: caracterizar a ideação suicida durante a vida em pessoas com transtornos relacionados a substâncias em tratamento nos Centros de Atenção Psicossocial Álcool e outras Drogas. Metodologia: estudo observacional e transversal realizado em três Centros de Atenção Psicossocial Álcool e outras Drogas III com 137 participantes. A coleta de dados ocorreu mediante aplicação de dois instrumentos: Columbia Suicide Severity Rating Scale e Addiction Severity Index – Version 6 e analisados descritivamente a partir de frequências absolutas e relativas, média e desvio padrão. Resultados: 112 (81,8%) participantes relataram ideação suicida alguma vez na vida; destes 55 (49,1%) tinham plano e intenção de executá-lo; 40 (35,8%) com pensamentos diários e muitas vezes ao dia e 56 (49,9%) com duração de até oito horas ou mais; 78 participantes (69,6%) consideravam a ideação suicida certamente como um meio de findar o sofrimento. Conclusão: observou-se elevada frequência de ideação suicida durante a vida em pessoas com transtornos relacionados a substâncias em tratamento, com variada gravidade e intensidade de acordo com aspectos sociodemográficos, econômicos e clínicos. Estes resultados podem subsidiar a prática profissional em saúde mental, possibilitando o desenvolvimento de estratégias preventivas.
O cupim da corrupção
Authors: Lafer Celso
Published: April 2023

The article discusses the relationship between ethics and politics with a focus on the impact of corruption on the functioning of the political system. Bearing in mind what has been recently happening in Brazil with the unfolding of the scandals dubbed Mensalão [Monthly Allowance] and Lava Jato [Car Wash], the ar ticle points out how corruption is both eating way trust in public institutions and compromising the role of public spirit in a democracy
É possível contribuir para a formação ética de nossos juristas? Desafios para o ensino jurídico no Brasil
Authors: Vieira Oscar Vilhena , Ghirardi José Garcez
Published: April 2023

This paper discusses the impact law schools can be expected to have on the ethical formation of their students, and puts forward a new proposal for teaching ethics. The f irst section e xamines traditional ways through which ethical education has been delivered in law schools; the second analy zes the interplay between interests, ethics and worldviews; and the third advocates a new pedagogy for teaching ethics in law schools, and suggests class debates on real cases are more likely to effectively contribute to the ethical formation of students than traditional lecture classes which still prevail in Brazil
Aspectos da sexualidade em indivíduos com traumatismo crânio–encefálico: revisão da literatura
Authors: Santos Filho Carlos Souto dos , Spizzirri Giancarlo
Published: April 2023

Os transtornos sexuais após lesão encefálica traumática são pouco abordados na literatura científica. O impacto na sexualidade dos pacientes, na parceria, na família e na sociedade ainda é pouco compreendido pelos profissionais de reabilitação. Diante disto, fez-se uma revisão da literatura sobre diversos aspectos da sexualidade após trauma de crânio. Este estudo teve por objetivo investigar a epidemiologia, a etiologia, os diagnósticos, a classificação, as modalidades terapêuticas que vêm sendo adotadas e o prognóstico dos transtornos sexuais de portadores de traumatismo craniano. Realizou-se uma revisão de 39 artigos selecionados nas seguintes bases de dados científicas: MEDLINE, Cochrane Library, LILACS e Scielo, usando como descritores: brain Injuries; traumatic brain Injury; sexuality, sexual behavior; sexual dysfunction psychological; sex education; sexual violence, referentes à pacientes maiores de 18 anos, de ambos os gêneros, no período de janeiro 1966 a maio 2011, nas línguas inglesa, espanhola e portuguesa. Alguns artigos e livros de referencia devido à relevância histórica neste assunto foram incluídos. Os autores abordaram os aspectos clínico-funcionais, psicológicos e sociais da sexualidade desses indivíduos. Conclusão: a avaliação completa por profissionais capacitados na área de sexualidade é recomendada durante o processo de reabilitação para uma melhor reintegração na sociedade. São necessários mais estudos para delinear as disfunções sexuais apresentadas por esses pacientes e suas parcerias, a fim de desenvolver estratégias de tratamento que contemplem suas necessidades possibilitando melhora no relacionamento, satisfação sexual e conseqüentemente na qualidade de vida.
Unidade de quedas: uma possibilidade de prevenção de quedas em idosos
Authors: Battistella Linamara Rizzo , Alfieri Fábio Marcon
Published: April 2023

As quedas são um problema de saúde pública entre os idosos. Como o envelhecimento populacional está ocorrendo rapidamente, são desejáveis intervenções nesta questão. O objetivo desta reflexão foi o de relatar um exemplo de uma Unidade de Quedas do Hospital Universitário Mútua de Terrassa/Barcelona/Espanha. Esta unidade possui atividades de avaliação e orientação dos fatores de risco de quedas e programa de exercícios para indivíduos com história de quedas, bem como para aqueles que possuem riscos de quedas. Acreditamos que programas como este que apresentam viabilidade operacional são necessários e devem ser incorporados por hospitais e centros de saúde para que haja diminuição das quedas e suas conseqüências.
Estudo da eficácia da terapia por onda de choque extracorpórea para o tratamento da dor no ombro hemiplégico
Authors: Abboud Fábio Coimbra Rodrigues , Cruz Filho Rodrigo da , Rocha Rafael Calanzani , Maure Marcos Vinícius Carvalho , Riberto Marcelo
Published: April 2023

A síndrome do ombro doloroso hemiplégico (SODH) consiste no surgimento de queixas dolorosas junto ao ombro do pacientes hemiplégico por acidente vascular cerebral (AVC), tem alta prevalência e relevante impacto na qualidade de vida por interferir na participação adequada aos programas de reabilitação, atividades de vida diária e relacionamento interpessoal. Sua etiologia é multifatorial e o tratamento adequado muitas vezes depende de uma abordagem multidisciplinar. A terapia por ondas de choque (TOC) utiliza ondas mecânicas para o controle da dor musculoesquelética e pode ser uma alternativa terapêutica nesta condição clínica.
Decanoato de Nadrolona para osteoporose pós-menopausa: revisão com busca sistemática
Authors: Camara Lucas Caseri , Góis Aecio Flavio Teixeira de , Trevisani Virginia Moça , Civile Vinicius , Suzigan Erika Magalhães
Published: April 2023

As fraturas osteoporóticas, em especial, as que ocorrem em decorrência das alterações pós-menopausa, têm a projeção de liderar as causas de dor, incapacidade, institucionalização e aumento da morbimortalidade até o ano de 2025; com expectativa de despender USD 25 bilhões. Visando o aumento da densidade óssea, diversas medicações estão sendo utilizadas: uma delas é o Decanoato de Nandrolona (DN). Apesar de evidências científicas prévias para o uso dessa droga, um esteróide anabólico, esta não está incluída nas opções de tratamentos recomendados. O Decanoato de Nandrolona (DN) age no aumento da densidade mineral óssea ao aumentar a atividade osteoblástica e diminuir atividade dos osteoclastos.
Testosterona e acidente vascular encefálico isquêmico
Authors: Camara Lucas Caseri , Suzigan Erika Magalhães , Góis Aecio Flavio Teixeira de , Trevisani Virginia Moça
Published: April 2023

Níveis de testosterona sérica já foram relacionados a piora de fatores hematológicos, função e envelhecimento vascular, contribuindo potencialmente para formação de trombos. Com o envelhecimento, dados epidemiológicos mostram declínio dos níveis de testosterona, prejuízo da função vascular e aumento da incidências de doenças vasculares, como o Acidente Vascular Encefálico (AVE).
Existe correlação entre atividade elétrica muscular e cerebral após treino com terapia de contensão do membro superior e tronco em sujeitos com acidente vascular encefálico?
Authors: Oliveira Viviane Aparecida de , Dias Miqueline Pivoto Farias , Mendes Aline de Paula , Santos Adriana Teresa Silva , Reis Luciana Maria dos , Terra Andreia Maria Silva Vilela
Published: April 2023

Objetivo: Verificar se existe correlação entre excitabilidade central e periférica após o treino com a terapia de restrição induzida do movimento (TRIM) modificada associada a contensão de tronco para o membro superior parético após Acidente Vascular Encefálico (AVE). Métodos: Ensaio clínico controlado onde vinte e dois voluntários foram selecionados e randomizado em dois grupos: Grupo Controle (GC) e Grupo Experimental (GE). Eles foram avaliados por meio do eletroencefalograma (EEG) e da eletromiografia (EMG) de superfície. Os canais EEG analisados foram: Canal Ântero Frontal (AF3/AF4); Canal Frontal região medial (F7/F8); Canal Frontal região lateral (F3/F4); Canal Fronto Central (FC5/FC6); Região temporal (T7/T8); Região occipital (O1/O2). Os músculos analisados com EMG foram bíceps braquial, flexores e extensores de punho. As avaliações foram realizada durante a atividade, por dez minutos. O GE recebeu o treinamento com TRIM modificada uma hora por dia, duas semanas consecutivas. Resultados: O GC (pós-intervenção) apresentou correlação moderada negativa (r= -0,69; p= 0,02) do canal Fa com extensor de punho. O GE apresentou (pré-intervenção) correlação moderada negativa do canal AF (r= -0,68; p= 0,02) e FC (r= -0,71; p= 0,01) com flexor de punho. Na pós intervenção apresentou correlação positiva do canal Fa (r= 0,61; p= 0,04) com o extensor de punho. Conclusão: A TRIM modificada associada à contensão de tronco para o membro superior parético apresentou correlação positiva do canal Fa com o músculo extensor de punho e o grupo não treinado apresentou correlação negativa do canal Fa com extensor de punho.
Avaliação muscular do joelho e funcional de membros inferiores de jovens atletas de voleibol do sexo feminino
Authors: Rodrigues Ana Paula , Fabian Geiziane de Fátima , Cecconi Matheus Pauletti , Eberle Elias Franzoi , Balbinot Guilherme Faria , Viecelli Andressa , Tadiello Gerson Saciloto , Bonetti Leandro Viçosa
Published: April 2023

Os desequilíbrios musculares e funcionais são analisados na tentativa de prevenir lesões e melhorar os desempenhos de atletas. Objetivo: Analisar o desempenho muscular de extensores e flexores de joelho e o desempenho funcional dos membros inferiores de atletas de voleibol. Métodos: Quinze atletas da categoria sub-16 de voleibol feminino da Universidade de Caxias do Sul foram avaliadas. Para análise do desempenho muscular, as variáveis pico de torque de extensores e flexores de joelho, e razão convencional de extensores/flexores, foi utilizado o dinamômetro isocinético. Já para a análise funcional dos membros inferiores, foram utilizados os testes Hop tests e Y balance test (YBT). Resultados: Na comparação entre membro dominante (MD) e não dominante (MND), não houve diferenças estatisticamente significativas na análise do pico de torque; já na razão flexores/extensores foi encontrada diferença significativa apenas à 240°/s. Entretanto, os valores médios da razão flexores/extensores encontrarem-se abaixo dos valores sugeridos pela literatura. As avaliações funcionais também não mostraram assimetrias entre os membros; no entanto, a pontuação composta do YBT apresentou-se abaixo dos valores normativos. Conclusão: A excelente simetria entre os membros pode ser justificada pela especificidade do esporte, pois os gestos esportivos do voleibol apresentam carácter simétrico durante as exigências físicas dos membros inferiores. No entanto, os baixos valores da razão flexores/extensores e da pontuação composta do YBT talvez não estejam diretamente relacionados a um risco maior de lesões já que os valores de referência utilizados são de atletas adultos.
Versão Brasileira do Questionário de Satisfação com a Prótese (SAT-PRO/Br): validade estrutural, validade concorrente, consistência interna e estabilidade
Authors: Gutierres Filho Paulo José Barbosa , Silva Diego Rodrigues Pimentel da , Ferreira Leonardo Dimas , Lima David Braga , Fernandes Jorge Manuel Gomes de Azevedo , Silva Rudney da
Published: April 2023

Objetivo: Analisar as propriedades psicométricas do SAT-PRO/Br através da observação da validade estrutural e concorrente, consistência interna e estabilidade. Método: A amostra deste estudo transversal foi consecutiva, não probabilística, constituída por 128 idosos com amputação de membro inferior, com amputação unilateral e que utilizavam a prótese a pelo menos um ano. As propriedades psicométricas foram avaliadas através da validade estrutural (Comparative Fit Index- CFI, Tucker-Lewis Index-TLI, Root Means Square Error of Approximation-RMSEA e  Standardized Root Mean Residual-SRMR), validade concorrente utilizando a versão brasileira da Trinity Amputation and Prosthesis Experience Scales - Revised (TAPES-R), além da  consistência interna dos itens pelo alfa de Cronbach e estabilidade pelo Intraclass Correlacion Coefficient (ICC). Resultados: O SAT-PRO/Br e a TAPES-R apresentaram forte correlação em relação a validade concorrente. A estabilidade variou de 0,85 a 0,91 através do ICC. Os índices de modelo do ajuste apresentaram valores de CFI de 0,991, TLI de 0,989, RMSEA de 0,045 e o SRMR de 0,074.  Observou-se uma boa consistência interna com alfa de Cronbach total de 0.91. Conclusão: As propriedades psicométricas deste estudo sobre o SAT-PRO/Br levam a concluir que esta versão é válida, confiável e apresenta boa consistência interna e estabilidade para ser aplicada em idosos brasileiros amputados de membro inferior.
Comemoração do jubileu de ouro da Academia Brasileira de Medicina de Reabilitação - ABMR
Authors: Leitão Ana Valéria Araujo
Published: April 2023

Noticiamos com muita alegria o cinquentenário da Academia de Medicina de Reabilitação - ABMR, ocasião que todos os médicos Fisiatras prestam uma homenagem ao fundador reproduzindo a historia desta Academia nas palavra da Fisiatra Ana Valéria Araujo Leitão.
Sex Differences in Outcomes of Ticagrelor Therapy With or Without Aspirin After Percutaneous Coronary Intervention in Patients With Acute Coronary Syndrome: A Post Hoc Secondary Analysis of the TICO Randomized Clinical Trial
Authors: Lee Bom , Lee Seung-Jun , Kim Byeong-Keuk , Lee Yong-Joon , Hong Sung-Jin , Ahn Chul-Min , Kim Jung-Sun , Ko Young-Guk , Choi Donghoon , Hong Meyong-Ki , Jang Yangsoo
Published: April 2023

Background:

We sought to explore the sex differences in clinical outcomes among patients with acute coronary syndrome treated with ticagrelor monotherapy after ticagrelor-based 3-month versus 12-month dual-antiplatelet therapy.

Methods:

This was a post hoc analysis of the TICO trial (Ticagrelor Monotherapy After 3 Months in the Patients Treated With New Generation Sirolimus-Eluting Stent for Acute Coronary Syndrome; n=3056)—a randomized controlled trial for patients with acute coronary syndrome treated with drug-eluting stent. The primary outcome was a net adverse clinical event (composite of major bleeding, death, myocardial infarction, stent thrombosis, stroke, or target-vessel revascularization) 1 year after drug-eluting stent implantation. Secondary outcomes included major bleeding and major adverse cardiac and cerebrovascular events.

Results:

There were 27.3% (n=628) women in the TICO trial; they were older with lower body mass index and higher prevalence of hypertension, diabetes, or chronic kidney disease than men. Compared with men, women had higher risk of net adverse clinical events (hazard ratio [HR], 1.89 [95% CI, 1.34–2.67]), major adverse cardiac and cerebrovascular events (HR, 1.69 [95% CI, 1.07–2.68]), and major bleeding (HR, 2.04 [95% CI, 1.25–3.35]). Among the groups stratified by sex and dual-antiplatelet therapy strategy, the incidences of primary and secondary outcomes were significantly different and the highest in women with ticagrelor-based 12-month dual-antiplatelet therapy (
P
<0.001). There was no significant heterogeneity in the impact of treatment strategy on the risks of primary and secondary outcomes between both sexes. Ticagrelor monotherapy was associated with a lower risk of the primary outcome in women (HR, 0.47 [95% CI, 0.26–0.85];
P
=0.02) and comparable in men (HR, 0.77 [95% CI, 0.52–1.14];
P
=0.19) without significant interaction (
P
for interaction, 0.18).

Conclusions:

After percutaneous coronary intervention for acute coronary syndrome, women demonstrated worse clinical outcomes than men. Ticagrelor monotherapy after 3-month dual-antiplatelet therapy was associated with significantly lower risk of net adverse clinical events in women without sex interaction.
Introducing the Bulletin of Phylogenetic Nomenclature
Authors: De Queiroz Kevin
Published: April 2023

With this inaugural issue, the International Society for Phylogenetic Nomenclature (ISPN) is launching its new journal, the Bulletin of Phylogenetic Nomenclature (BPN).  The inauguration of the Bulletin of Phylogenetic Nomenclature marks another important stage in the development of a phylogenetic approach to nomenclature for the general taxonomic reference system of biology, and it is the hope of the ISPN that this journal will help to facilitate and promote the adoption of that approach.
The Australia’s Foreign Relations Act and Australia’s Relationship with International Law
Authors: Clough Georgina
Published: April 2023

This article examines the consequences of the Australia’s Foreign Relations (State and Territory Arrangements) Act 2020 (Cth) (‘Foreign Relations Act’) for international law. It argues that the arrangements entered into by state, territory and local governments to which the Foreign Relations Act applies can be relevant to international law in three ways. First, they may relate indirectly to Australia’s international legal obligations. Second, they may be a means by which Australian subnational governments claim a role for themselves in governance on global issues. Third, as an exercise of diplomacy, they influence the relations Australia maintains with other nations and the way in which it participates in the international system. As the states and territories in particular become more assertive, including on international issues such as climate change, giving the Commonwealth complete control over such arrangements may impact Australia’s relationship with international law.
Long-term clinical results of using a posteromedial all-inside and anteromedial inside-out approach to repair unstable or irreducible bucket-handle medial meniscal tears
Authors: Keyhani Sohrab , Movahedinia Mohammad , LaPrade Robert F. , Qoreishy Mohamad , Vosoughi Fardis
Published: April 2023

Background

In irreducible bucket-handle medial meniscal tears (BHMMTs), the displaced central fragment is rotated 180° or more, meaning reduction is impossible without derotation of the tear. In chronic cases, the rotated meniscus is deformed and degenerated; thus, the issue of reproducibility and repairability arises. This study presents the clinical outcomes of chronic irreducible and unstable locked BHMMTs. Radiological outcomes were also evaluated using magnetic resonance imaging (MRI).

Methods

This is a retrospective study of 37 patients with 11 cases of irreducible BHMMT and 26 cases of reducible but unstable chronic BHMMT who underwent operations between 2011 and 2016. Posterior arthroscopy was performed after temporary meniscus fixation using a provisional needle fixation technique. After the posterior repair with vertical mattress sutures was completed using an all-inside technique, the classic outside-in technique was performed for the anterior third of the meniscus. The Lysholm, International Knee Documentation Committee (IKDC), and Tegner activity scores were obtained from all patients before surgery and at the latest follow-up. Radiological evaluations were performed using MRI before surgery and at 6 months postoperatively.

Results

Between 2011 and 2016, a total of 37 consecutive patients with irreducible and chronic BHMMTs underwent surgery. The average postoperative follow-up was 7.2 ± 1.4 years (mean ± SD). Postoperative Lysholm (89.57 ± 2.7) and IKDC (87.22 ± 3.2) scores improved significantly at the last follow-up when compared with the pre-operative scores (38.44 ± 4.5 and 23.52 ± 7.8, respectively). According to the Tegner activity scale, patients’ postoperative activity levels remained unchanged compared to preoperative levels at the last follow-up.

Conclusion

Posterior knee arthroscopy with the all-inside posterior suture and inside-out anteromedial suture technique presented in this study yielded excellent clinical outcomes when used to repair chronic irreducible or unstable BHMMTs.

Level of evidence: Level IV.
Relation between ocular paraneoplastic syndromes and Immune Checkpoint Inhibitors (ICI): review of literature
Authors: Casselman Pauline , Jacob Julie , Schauwvlieghe Pieter-Paul
Published: April 2023

Purpose

To describe different ocular paraneoplastic syndromes in patients treated with Immune Checkpoint Inhibitors (ICI), its relation with different types of ICI and different types of tumors, and its implications for treatment.

Methods

A comprehensive review of the literature was performed.

Results

Patients treated with ICI can present with different ocular paraneoplastic syndromes, such as Carcinoma Associated Retinopathy (CAR), Melanoma Associated Retinopathy (MAR) and paraneoplastic Acute Exudative Polymorphous Vitelliform Maculopathy (pAEPVM). In literature, the different types of paraneoplastic retinopathy are mostly related to different types of primary tumors, with MAR and pAEPVM seen in melanoma, and CAR in carcinoma. Visual prognosis is limited in MAR and CAR.

Conclusion

Paraneoplastic disorders result from an antitumor immune response against a shared autoantigen between the tumor and ocular tissue. ICI enhance the antitumor immune response, which can lead to increased cross-reaction against ocular structures and unmasking of a predisposed paraneoplastic syndrome. Different types of primary tumors are related to different cross-reactive antibodies. Therefore, the different types of paraneoplastic syndromes are related to different types of primary tumors and are probably unrelated to the type of ICI. ICI-related paraneoplastic syndromes often lead to an ethical dilemma. Continuation of ICI treatment can lead to irreversible visual loss in MAR and CAR. In these cases overall survival must be weighed against quality of life. In pAEPVM however, the vitelliform lesions can disappear with tumor control, which may involve continuation of ICI.
Using sensitivity analyses to understand bistable system behavior
Authors: Sreedharan Vandana , Bhalla Upinder S. , Ramakrishnan Naren
Published: April 2023

Background

Bistable systems, i.e., systems that exhibit two stable steady states, are of particular interest in biology. They can implement binary cellular decision making, e.g., in pathways for cellular differentiation and cell cycle regulation. The onset of cancer, prion diseases, and neurodegenerative diseases are known to be associated with malfunctioning bistable systems. Exploring and characterizing parameter spaces in bistable systems, so that they retain or lose bistability, is part of a lot of therapeutic research such as cancer pharmacology.

Results

We use eigenvalue sensitivity analysis and stable state separation sensitivity analysis to understand bistable system behaviors, and to characterize the most sensitive parameters of a bistable system. While eigenvalue sensitivity analysis is an established technique in engineering disciplines, it has not been frequently used to study biological systems. We demonstrate the utility of these approaches on a published bistable system. We also illustrate scalability and generalizability of these methods to larger bistable systems.

Conclusions

Eigenvalue sensitivity analysis and separation sensitivity analysis prove to be promising tools to define parameter design rules to make switching decisions between either stable steady state of a bistable system and a corresponding monostable state after bifurcation. These rules were applied to the smallest two-component bistable system and results were validated analytically. We showed that with multiple parameter settings of the same bistable system, we can design switching to a desirable state to retain or lose bistability when the most sensitive parameter is varied according to our parameter perturbation recommendations. We propose eigenvalue and stable state separation sensitivity analyses as a framework to evaluate large and complex bistable systems.
Preliminary clinical observation of double C-arm digital subtraction angiography guidance during transjugular intrahepatic portosystemic shunt placement
Authors: Chen Jianqiao , Bai Xiao , Wang Chunyan , Li Jihua , Xu Weiguo
Published: April 2023

Background

This study aimed to investigate the safety, preliminary clinical experience, and technical advantages of double C-arm digital subtraction angiography -assisted portal vein puncture for transjugular intrahepatic portosystemic shunt.

Methods

Clinical data of 25 patients with portal hypertension caused by liver cirrhosis were retrospectively analyzed from January 2021 to June 2022. The fluoroscopy time, puncture time, mean portosystemic pressure gradient, dose area product, and intraoperative and postoperative complications were recorded.

Results

Transjugular intrahepatic portosystemic shunt was performed in all 25 patients, with a success rate of 100%. The fluoroscopy time, puncture time, and dose area product were 33.6 ± 8.5 min, 9.1 ± 5.7 min, and 126 ± 53 Gy·cm2, respectively. The mean portosystemic pressure gradient decreased from 22.5 ± 6.3 mmHg to 10.5 ± 2.3 mmHg (p < 0.01). No serious intraoperative and postoperative complications were found.

Conclusion

Double C-arm digital subtraction angiography-assisted portal vein puncture is safe and feasible in transjugular intrahepatic portosystemic shunt operation. It can reduce the difficulty of the operation and possesses evident technical advantages.
Disseminated cryptococcosis with gastrointestinal involvement and false-negative cryptococcal antigen testing due to postzone phenomenon: a case report and review of the literature
Authors: Zimmet Alex N. , Cullen Grace D. , Mische Leah , Deftos Michael , Bogler Yael , Nguyen Nang L. , Ray Manoj
Published: April 2023

Background

Cryptococcosis is an increasingly common infection given the growing immunocompromised population worldwide. Cryptococcal antigen (CrAg) testing demonstrates excellent sensitivity and specificity and is the mainstay of diagnosis. However, there may be rare instances in which false-negative CrAg results can delay diagnosis and early treatment, which are critical to ensure positive outcomes.

Case presentation

A 31-year-old man living with HIV/AIDS who was not taking antiretroviral therapy was hospitalized with fever, diarrhea, and headaches. CD4 count on presentation was 71 cells/uL, and HIV viral load was 3,194,949 copies/mL. Serum CrAg testing was initially negative, however CSF CrAg performed several days later was positive at 1:40 and blood and CSF cultures grew Cryptococcus neoformans. Colonoscopy revealed mucosal papules throughout the sigmoid colon, and tissue biopsy showed yeast within the lamina propria consistent with GI cryptococcosis. Given the high burden of disease, the original serum CrAg specimen was serially diluted and subsequently found to be positive at 1:2,560, confirming the postzone phenomenon.

Conclusion

Cryptococcosis has a wide array of presentations including intraluminal GI disease, as seen in this patient. While serum CrAg testing displays excellent test characteristics, it is important for clinicians to be aware of the rare instances in which false-negative results may occur in the presence of excess antigen, as in this case.
Effects of temperature, rainfall, and El Niño Southern Oscillations on dengue-like-illness incidence in Solomon Islands
Authors: Andhikaputra Gerry , Lin Yu-Han , Wang Yu-Chun
Published: April 2023

Background

This study investigated associations between climate variables (average temperature and cumulative rainfall), and El Niño Southern Oscillation (ENSO) and dengue-like-illness (DLI) incidence in two provinces (Western and Guadalcanal Provinces) in Solomon Islands (SI).

Methods

Weekly DLI and meteorological data were obtained from the Ministry of Health and Medical Services SI and the Ministry of Environment, Climate Change, Disaster Management and Meteorology from 2015 to 2018, respectively. We used negative binomial generalized estimating equations to assess the effects of climate variables up to a lag of 2 months and ENSO on DLI incidence in SI.

Results

We captured an upsurge in DLI trend between August 2016 and April 2017. We found the effects of average temperature on DLI in Guadalcanal Province at lag of one month (IRR: 2.186, 95% CI: 1.094–4.368). Rainfall had minor but consistent effect in all provinces. La Niña associated with increased DLI risks in Guadalcanal Province (IRR: 4.537, 95% CI: 2.042–10.083), whereas El Niño associated with risk reduction ranging from 72.8% to 76.7% in both provinces.

Conclusions

Owing to the effects of climate variability and ENSO on DLI, defining suitable and sustainable measures to control dengue transmission and enhancing community resilience against climate change in low- and middle-developed countries are important.
Trends in Neisseria meningitidis serogroups amongst patients with suspected cerebrospinal meningitis in the meningitis belt of Ghana: a 5-year retrospective study
Authors: Azure Stebleson , Abdul-Karim Abass , Abubakari Braimah Baba , Eleeza John B. , Agboyie Daron Davies A. , Weyori Enoch Weikem , Choi Jun Yong
Published: April 2023

Background

Serogroup A Neisseria meningitidis was the major cause of meningococcal meningitis epidemics in the African meningitis belt before 2010 when the monovalent meningococcal A conjugate vaccine (MenAfriVac) was introduced in the region. Therefore, this study aimed to establish the trends in N. meningitidis serogroups from 2016 to 2020 in Ghana’s meningitis belt.

Methods

Polymerase chain reaction (PCR) confirmed laboratory results of suspected cases of cerebrospinal meningitis from January, 2016 to March, 2020 were obtained from the Tamale Public Health Laboratory. The data were subjected to trend analysis using Statistical Package for the Social Sciences version 25. Differences between discrete variables were analyzed using the Cochran–Armitage trend test.

Results

Of the 2,426 suspected cases, 395 (16.3%) were confirmed positive for N. meningitidis using PCR. Serogroup X showed a significant upward trend (P < 0.01), and serogroup W showed a downward trend (P < 0.01). However, no significant trend was observed for any other serogroup.

Conclusion

This study showed the emergence of serogroup X, a non-vaccine type, as the predominant N. meningitidis serogroup in the wake of a declining serogroup W in Ghana’s meningitis belt.
Coexistence of COVID-19, Pseudomonas, and thoracic actinomycosis in a cystic bronchiectasis case
Authors: Afsin Emine , Kucuk Furkan , Ozcelik Hüsna , Haktanır Muhammed Yavuz
Published: April 2023

Actinomycosis often leads to cervicofacial infections, but thoracic involvement may also occur. However, the development of empyema is rare. While being followed up with the diagnosis of asthma and bronchiectasis, our case was hospitalized for infected bronchiectasis. As empyema developed in the follow-up, the pleural effusion was drained by tube thoracostomy. Actinomycosis was diagnosed through pleural effusion cytology. Growth of Pseudomonas aeruginosa was observed in sputum culture, and SARS-CoV2 RT-PCR was also positive in nasopharyngeal sampling. Polymicrobial agents can often be detected in actinomycosis. Actinomycosis cases have also been reported in the post-COVID period. Our case is presented since it would be the first in the literature regarding the coexistence of COVID-19, Pseudomonas, and thoracic Actinomycosis (empyema).
The effects of the prognostic biomarker SAAL1 on cancer growth and its association with the immune microenvironment in lung adenocarcinoma
Authors: Tong Song , Jiang Ni , Wan Jun-Hao , Chen Chong-Rui , Wang Si-Hua , Wu Chuang-Yan , Guo Qiang , Xiao Xiao-Yue , Huang Huan , Zhou Ting
Published: April 2023

Background

Inhibition of Serum Amyloid A-like 1 (SAAL1) expression could inhibit cancer progression and improve the prognosis of cancer patients. At present, the correlation between SAAL1 and lung adenocarcinoma (LAC) remains unclear. Therefore, this study surveyed the worth and pathway of SAAL1 in LAC progression and immunity.

Methods

Bioinformatics and immunohistochemistry were used to identify the SAAL1 expression in LAC. The roles of SAAL1 expression in the existence values of LAC patients were explored, and the nomograms were constructed. Clinical values of SAAL1 co-expressed genes were evaluated by COX regression, survival, and Receiver operating characteristic (ROC) analysis. EDU and western blotting methods were used to inquiry the functions and pathways of the SAAL1 in cell growths. The correlation between the SAAL1 level and immune microenvironment was visualized using correlation research.

Results

SAAL1 level was elevated in LAC tissues, and was observed in cancer tissues of dead patients. SAAL1 overexpression had something to do with shorter overall survival, progression-free interval, and disease-specific survival in LAC. The area under the curve of SAAL1 was 0.902 in normal tissues and cancer tissues. Inhibition of SAAL1 expression could inhibit cancer cell proliferation, which may be related to the decreased expression of cyclin D1 and Bcl-2 proteins. In LAC, SAAL1 level had something to do with stromal, immune, and estimate scores, and correlated with macrophages, T cells, Th2 cells, CD8 T cells, NK CD56dim cells, DC, eosinophils, NK CD56bright cells, pDC, iDC, cytotoxic cells, Tgd, aDC cells, B cells, Tcm, and TFH levels. SAAL1 overexpression had something to do with existence values and the immunity in LAC.

Conclusions

Inhibition of SAAL1 expression could regulate cancer growth via cyclin D1 and Bcl-2. SAAL1 is a promising prognostic biomarker in LAC patients.
Effect of eye shield and ear muffs on pain intensity during venous blood sampling in premature infants: a clinical trial study
Authors: Shykhveisi Fatemeh , Jafarian Amiri Roghayeh , Zabihi Ali , Haghshenas Mojaveri Mohsen , Arzani Afsaneh , Chehrazi Mohammad , Chari Zahra Valizadeh
Published: April 2023

Background

Today, due to the side effects of drugs, there is a greater desire to use non-pharmacological interventions to relieve pain caused by painful procedures. Using non-pharmacological interventions in combination is more effective than using them alone in relieving the pain of infants. Reducing sensory and environmental stimuli such as visual and auditory stimuli is one of the non-pharmacological methods to relieve pain. The aim of this study was to investigate the effect of using eye shield and ear muffs on pain intensity during venous blood sampling of premature infants.

Methods

In this clinical trial study, 148 premature neonates admitted to the Neonatal Intensive Care Unit of Rouhani and Children Hospitals in Babol were randomly assigned to four groups of 37. Fifteen minutes before intravenous blood sampling until 15 min later, in the first group, eye shield; in the second group, ear muffs, and in the third group, eye shield plus ear muffs were used. In the fourth group (control), blood sampling was performed routinely. NIPS pain scale and demographic questionnaire were used to collect the data.

Results

The results showed that during the venous blood sampling was a significant difference between the mean pain intensity of neonatal in the eye shield plus ear muffs group (3.14 ± 0.71), the ear muffs group (4.43 ± 1.21), the eye shield group (5.41 ± 1.04).) and the control group (5.94 ± 0.84) (P = 0.001). Moreover, after the venous blood sampling, there was a significant difference between the mean neonatal pain intensity in the eye shield plus ear muffs group (1.19 ± 0.39), the ear muffs group (1.43 ± 0.50), the eye shield group (1.33 ± 0.37) and the control group (1.89 ± 0.90) (P = 0.001).

Conclusions

In this study, the pain severity during and after venous blood sampling in the ear muffs plus eye shield was lower than in other groups. Therefore, a combination of ear muffs and eye shield is recommended as a better pain reliever when performing venous blood sampling in premature infants.