bluuebunny/crossref_metadata_2025 huggingface dataset:
The Optical Forces and Torques Exerted by Airy Light-Sheet on Magnetic Particles Utilized for Targeted Drug Delivery
Authors: Song Ningning , Chen Shiguo , Wang Hao , He Xinbo , Wei Bing , Li Renxian , Zhang Shu , Xu Lei
Published: November 2024

The remarkable properties of magnetic nanostructures have sparked considerable interest within the biomedical domain, owing to their potential for diverse applications. In targeted drug delivery systems, therapeutic molecules can be loaded onto magnetic nanocarriers and precisely guided and released within the body with the assistance of an externally applied magnetic field. However, conventional external magnetic fields generated by permanent magnets or electromagnets are limited by finite magnetic field gradients, shallow penetration depths, and low precision. The novel structured light field known as the Airy light-sheet possesses unique characteristics such as non-diffraction, self-healing, and self-acceleration, which can potentially overcome the limitations of traditional magnetic fields to some extent. While existing studies have primarily focused on the manipulation of dielectric particles by Airy light-sheet, comprehensive analyses exploring the intricate interplay between Airy light-sheet and magnetic nanostructures are currently lacking in the literature, with only preliminary theoretical discussions available. This study systematically explores the mechanical response of magnetic spherical particles under the influence of Airy light-sheet, including radiation forces and spin torques. Furthermore, we provide an in-depth analysis of the effects of particle size, permittivity, permeability, and incident light-sheet parameters on the mechanical effects. Our research findings not only offer new theoretical guidance and practical references for the application of magnetic nanoparticles in biomedicine but also provide valuable insights for the manipulation of other types of micro/nanoparticles using structured light fields.
Extended-Spectrum Beta-Lactamase-Producing and Multidrug-Resistant Escherichia coli and Klebsiella spp. from the Human–Animal–Environment Interface on Cattle Farms in Burkina Faso
Authors: Soma Djifahamaï , Bonkoungou Isidore Juste Ouindgueta , Garba Zakaria , Diarra Fatimata Bintou Josiane , Somda Namwin Siourimè , Nikiema Marguerite Edith Malatala , Bako Evariste , Sore Souleymane , Sawadogo Natéwindé , Barro Nicolas , Haukka Kaisa
Published: November 2024

Extended-spectrum beta-lactamase (ESBL)-producing and multidrug-resistant Enterobacterales pose a major threat to both human and animal health. This study assessed the prevalence of ESBL-producing Escherichia coli (ESBL-Ec) and Klebsiella spp. (ESBL-K) on cattle farms in Ouagadougou, Burkina Faso, using a One Health approach. From May 2021 to September 2022, cattle faeces, farmers’ stools, their drinking water and farm soil samples were collected from semi-intensive and traditional farms. An ESBL-selective medium was used to obtain resistant isolates, which were further characterised using biochemical tests. Antimicrobial susceptibility testing was performed using the Kirby–Bauer disc diffusion method. ESBL-Ec and/or ESBL-K were detected in 188 of 322 samples (58.0%). The prevalence of ESBL-Ec isolates was 42.2% (136/322) and that of ESBL-K isolates was 24.5% (79/322). Notably, 156 of the 188 ESBL isolates (83.0%) exhibited multidrug resistance. The highest resistance rates were observed against tetracycline and cotrimoxazole. Importantly, no isolates showed resistance to meropenem, which was used to test for carbapenem resistance. This study highlights the presence of ESBL-Ec and ESBL-K among the humans, animals and environment of the cattle farms. Good hygiene and biosafety practices are essential to limit the potential spread of multidrug-resistant bacteria between different interfaces on farms.
Bacterial Degradation of Ibuprofen: Insights into Metabolites, Enzymes, and Environmental Fate Biodegradation of Ibuprofen by Achromobacter Species
Authors: Fetyan Nashwa A. H. , Asair Ayan A. , Ismail Ismail M. , Elsakhawy Tamer A. , Elnagdy Sherif M. , Mohamed Mahmoud S. M.
Published: November 2024

In recent years, pharmaceuticals have emerged as pollutants due to their incomplete degradation in sewage treatment plants and their ability to cause physiological problems in humans even at low doses. Understanding the environmental fate of pharmaceutical pollutants and the mechanisms involved in their degradation is crucial for developing strategies to mitigate their impact on ecosystems and human health. In this study, the degradation of pharmaceutical compound ibuprofen was achieved by employing two bacterial strains, Achromobacter spanius strain S11 and Achromobacter piechaudii S18, previously isolated from contaminated water. These strains were capable of degrading ibuprofen as their sole carbon source. The study aimed to identify intermediate metabolites, determine the enzymes involved, and detect specific genes related to ibuprofen degradation. Different concentrations of ibuprofen, temperatures, and pH levels were tested. Both A. spanius S11 and A. piechaudii S18 successfully degraded ibuprofen. A. spanius S11 showed a degradation efficiency of 91.18% after only 72 h and reached 95.7% after 144 h, while A. piechaudii S18 exhibited degradation efficiencies of 72.39% and 73.01% after three and seven days, respectively. The LC-MS technique was used to identify biodegradation metabolites produced by A. spanius S11. The results indicated that the first step was hydroxylation followed by oxidation via the combination of monooxygenases that catalyze the C-H hydroxylation and dehydrogenases. Furthermore, the detection of intermediate metabolites of trihydroxyibuprofen suggested that the biodegradation of ibuprofen by A. spanius S11 can occur through multiple mechanisms. The highest enzyme activities were recorded for catechol 1,2-dioxygenase, 4.230 ± 0.026 U/mg, followed by laccase, 2.001 ± 0.215 U/mg. This study demonstrates the potential of Achromobacter strains, particularly A. spanius (S11), in degrading ibuprofen. These findings provide insights into the ibuprofen degradation process, intermediate metabolites, and relevant genes.
Effects of Perennial Alfalfa on the Structure and Function of Soil Micro-Food Webs in the Loess Plateau
Authors: Li Liangliang , Tian Jianxia , Luo Zhuzhu , Li Lingling , Niu Yining , Haider Fasih Ullah , Nian Lili , Zhang Yaoquan , He Renyuan , Liu Jiahe
Published: November 2024

The Loess Plateau is one of the most vulnerable areas in the world. Numerous studies have been conducted to investigate alfalfa fields with different planting years. Soil microorganisms and nematodes are vital in ecosystem functionality and nutrient cycling. Therefore, comprehending their response to alfalfa fields with varying years of planting is essential for predicting the direction and trajectory of degradation. Alfalfa fields with different planting years (2 years, 9 years, and 18 years) were used as the research object, and farmland was used as the control (CK). High-throughput sequencing and morphological methods determined the community composition of microorganisms and nematodes. Carbon metabolic footprints, correlation networks, and structural equations were used to study soil microorganisms and nematode interactions. Principal component analysis (PCA) results showed that alfalfa fields with different planting years significantly impacted soil microorganisms and nematode community structures. Planting alfalfa significantly increased the nematode channel ratio (NCR) and Wasilewska index (WI), but significantly reduced the soil nematode PPI/MI and dominance (λ). The correlation network results indicated that, for the 2-year and 18-year treatments, the total number of links and positive links are higher than other treatments. Conversely, the 9-year treatment had fewer positive links and more negative links compared to other treatments. Additionally, the keystone species within each network varied based on the treatment years. Structural equation results show that alfalfa planting years directly impact soil fungal community structure and plant-parasitic nematodes’ carbon metabolism omnivorous-predatory nematodes. Furthermore, the carbon metabolism of omnivorous-predatory nematodes directly influences soil organic carbon fixation. Moreover, as the duration of alfalfa planting increases, the metabolic footprint of plant-parasitic nematodes decreases while that of omnivorous-predatory nematodes rises. Among treatments varying in alfalfa planting durations, the 9-year treatment exhibited the most incredible energy conversion and utilization efficiency within the soil food web, demonstrating the most stable structure. This study reveals optimal alfalfa planting duration for soil ecosystem stability in the Loess Plateau. Future research should explore sustainable crop rotations and alfalfa–soil–climate interactions for improved agricultural management.
Biodegradation of Crude Oil and Aniline by Heavy Metal-Tolerant Strain Rhodococcus sp. DH-2
Authors: Luo Zetian , Ma Jiajun , Huang Lei , Li Dahui , Gao Guohui , Zhao Yihe , Antunes Agostinho , Li Meitong
Published: November 2024

Aniline and crude oil are common environmental pollutants that present a significant risk to both the ecological and human health environments. The implementation of efficacious bioremediation strategies is imperative for the elimination of these contaminants. In this study, a bacterial strain designated DH-2 was isolated from soil contaminated with aniline. The strain was identified as belonging to the genus Rhodococcus. The optimal conditions for the growth and aniline degradation by strain DH-2 were determined to be pH 8.0 and 35 °C, respectively. Under these conditions, the degradation rate of aniline at a concentration of 1000 mg/L exceeded 90% within 36 h. Even in the presence of 4% NaCl, the degradation rate remained above 60%. HPLC–MS analysis revealed that the aniline degradation pathway of strain DH-2 follows the catechol pathway. Additionally, strain DH-2 is capable of utilizing crude oil as the sole carbon source, achieving a degradation rate of 91.0% for 2% crude oil concentration within 4 days. In soil modeling experiments, strain DH-2 was observed to degrade aniline and crude oil under triple stress conditions, including 1000 mg/L aniline, 2% crude oil, and 20 mg/L Fe(II) or Pb(II). Complete degradation of aniline and crude oil was achieved after 3 days and 12 days, respectively. The addition of Fe(II) or Pb(II) ions was found to enhance the degradation ability of DH-2. These results demonstrate that strain DH-2 is an extremely effective biodegradable strain, with potential applications in the remediation of environments contaminated with aniline and crude oil, even in the presence of heavy metals.
Mechanistic Insight into the Anti-Bacterial/Anti-Biofilm Effects of Low Chlorhexidine Concentrations on Enterococcus faecalis—In Vitro Study
Authors: Sebbane Nathanyel , Abramovitz Itzhak , Kot-Limon Nurit , Steinberg Doron
Published: November 2024

Background: Endodontic treatment failures are often linked to the persistence of Enterococcus faecalis in the root canal system. This study aimed to investigate the antibacterial/antibiofilm mechanism of chlorhexidine (CHX), particularly at low concentrations, against E. faecalis, to improve endodontic treatment protocols. Methods: The antibacterial activity of CHX (0.125–20 μg/mL) was evaluated against E. faecalis ATCC 29212 using various assays, including planktonic growth inhibition, colony-forming units (CFUs), membrane permeability and potential assays, high-resolution scanning electron microscopy (HR-SEM), confocal laser scanning microscopy of biofilms, biomass and metabolic activity assays on matured biofilm, and quantitative real-time PCR for gene expression. Statistical analysis was performed using Student’s t-test and ANOVA. Results: CHX demonstrated concentration-dependent inhibition of E. faecalis, significantly reducing planktonic growth and CFUs. Membrane assays showed increased permeability and depolarization, indicating damage. HR-SEM revealed morphological changes, such as pore formation, while confocal microscopy showed a reduction in biofilm mass and extracellular substances. Gene expression analysis indicated the downregulation of virulence genes and upregulation of stress response genes. Conclusions: CHX at low concentrations disrupts E. faecalis at multiple levels, from membrane disruption to gene expression modulation, affecting mature biofilm. These findings support the refinement of endodontic disinfection protocols to reduce microbial persistence.
A Comparative Analysis of the Ocular Microbiome: Insights into Healthy Eyes and Anophthalmic Sockets
Authors: Zamorano-Martín Francisco , Chumaceiro Guillermo , Navarro-Torres Pablo , Borroni Davide , Urbinati Facundo , Molina Ángel , Paytuví-Gallart Andreu , Rocha-de-Lossada Carlos
Published: November 2024

The purpose of this study is to characterize the ocular surface microbiota of patients with an anophthalmic cavity. An eNAT with 1 mL of Liquid Amies Medium was used to collect samples. Microbial DNA from anophthalmic socket and healthy fellow control eye samples was isolated and sequenced. Raw reads were analyzed with GAIA (v 2.02). The richness and Shannon alpha diversity metrics, as well as Bray–Curtis beta diversity and Wilcoxon signed-rank test values, were computed with R packages such as phyloseq, mia, or DESeq2 to allow for microbiome analysis. Principal coordinate analysis (PCoA) was performed using the function plotReducedDim from the R package scater. The different taxonomic profiles were described under the concept of eye community state type (ECST). The microbiomes of both eyes from 25 patients with an anophthalmic cavity were analyzed in this study. While the microbial communities of paired eyes from the same patients showed notable dissimilarity, no consistent patterns emerged when comparing healthy eyes to anophthalmic sockets. Alpha diversity values did not significantly differ between healthy eyes and anophthalmic socket samples, though there was considerable variability within each group. Notably, anophthalmic socket samples generally exhibited lower abundances of genera such as Staphylococcus, Enterococcus, Paenibacillus, and Sediminibacterium compared to their healthy counterparts. Microbial variability between healthy eyes and anophthalmic sockets may be due to anatomical differences. Further research is needed to determine whether patients without anophthalmic sockets exhibit similar microbiome patterns in both eyes.
3D Inversion and Interpretation of Airborne Multiphysics Data for Targeting Porphyry System, Flammefjeld, Greenland
Authors: Jorgensen Michael , Zhdanov Michael S. , Gribenko Alex , Cox Leif , Sabra Henrik E. , Prikhodko Alexander
Published: November 2024

The exploration of porphyry deposits in Greenland has become increasingly important due to their significant economic potential. We utilized total magnetic intensity (TMI) and mobile magnetotelluric (MobileMT) airborne data to delineate potential porphyry mineralization zones. The TMI method was employed to map variations in the Earth’s magnetic field caused by subsurface geological features, including mineral deposits. By analyzing anomalies in TMI data, potential porphyry targets were identified based on characteristic magnetic signatures associated with mineralized zones. Complementing TMI data, MT airborne surveys provided valuable insights into the electrical conductivity structure of the subsurface. Porphyry deposits exhibited distinct conductivity signatures due to the presence of disseminated sulfide minerals, aiding in their identification and delineation. Integration of the TMI and MobileMT datasets allowed for a comprehensive assessment of porphyry exploration targets in Flammefjeld. The combined approach facilitates the identification of prospective areas with enhanced geological potential, optimizing resource allocation and exploration efforts. Overall, this study demonstrates the efficacy of integrating TMI and MobileMT airborne data for porphyry exploration in Greenland, offering valuable insights for mineral exploration and resource development in the region.
A Reproducible Protocol for the Isolation of Malaria-Derived Extracellular Vesicles by Differential Centrifugation
Authors: Opadokun Tosin , Rohrbach Petra
Published: November 2024

Over the last few decades, malaria-derived extracellular vesicles (EVs) have gained increasing interest due to their role in disease pathophysiology and parasite biology. Unlike other EV research fields, the isolation of malaria EVs is not standardized, hampering inter-study comparisons. Most malaria EV studies isolate vesicles by the “gold-standard” technique of differential (ultra)centrifugation (DC). Here, we describe in detail an optimized and reproducible protocol for the isolation of malaria-derived EVs by DC. The protocol begins with a description of cultivating high-parasitemia, synchronous P. falciparum cultures that are the source of EV-containing conditioned culture media. The isolation protocol generates two EV subtypes, and we provide details of characterizing these distinct subtypes by analyzing human and parasite proteins by Western blot analysis. We identify some of these proteins as suitable markers for malaria EV subpopulations and subtypes.
Identifying and Removing Fraudulent Attempts to Enroll in a Human Health Improvement Intervention Trial in Rural Communities
Authors: Hanson Karla L. , Marshall Grace A. , Graham Meredith L. , Villarreal Deyaun L. , Volpe Leah C. , Seguin-Fowler Rebecca A.
Published: November 2024

Using the internet to recruit participants into research trials is effective but can attract high numbers of fraudulent attempts, particularly via social media. We drew upon the previous literature to rigorously identify and remove fraudulent attempts when recruiting rural residents into a community-based health improvement intervention trial. Our objectives herein were to describe our dynamic process for identifying fraudulent attempts, quantify the fraudulent attempts identified by each action, and make recommendations for minimizing fraudulent responses. The analysis was descriptive. Validation methods occurred in four phases: (1) recruitment and screening for eligibility and validation; (2) investigative periods requiring greater scrutiny; (3) baseline data cleaning; and (4) validation during the first annual follow-up survey. A total of 19,665 attempts to enroll were recorded, 74.4% of which were considered fraudulent. Automated checks for IP addresses outside study areas (22.1%) and reCAPTCHA screening (10.1%) efficiently identified many fraudulent attempts. Active investigative procedures identified the most fraudulent cases (33.7%) but required time-consuming interaction between researchers and individuals attempting to enroll. Some automated validation was overly zealous: 32.1% of all consented individuals who provided an invalid birthdate at follow-up were actively contacted by researchers and could verify or correct their birthdate. We anticipate fraudulent responses will grow increasingly nuanced and adaptive given recent advances in generative artificial intelligence. Researchers will need to balance automated and active validation techniques adapted to the topic of interest, population being recruited, and acceptable participant burden.
Leveraging Interactive Evolutionary Computation to Induce Serendipity in Informal Learning
Authors: Inoue Satoko , Ayedoun Emmanuel , Takenouchi Hiroshi , Tokumaru Masataka
Published: November 2024

Serendipitous learning, characterized by the discovery of new insights and unexpected connections, is recognized as a valuable educational experience that stimulates critical thinking and self-regulated learning. While there have been limited efforts to develop serendipity-oriented recommender systems in education, these systems often fall short in supporting learners’ agency, that is, the sense of ownership and control over their learning journey. In this paper, we introduce an Interactive Evolutionary Computation (IEC)-driven recommender system designed to empower learners by granting them control over their learning experiences while offering recommendations that are both novel and unexpected yet aligned with their interests. Our proposed system leverages an Interactive Genetic Algorithm in conjunction with Knowledge Graphs to dynamically recommend learning content, with a focus on the history of scientific discoveries. We conducted both numerical simulations and experimental evaluations to assess the effectiveness of our content optimization algorithm and the impact of our approach on inducing serendipity in informal learning environments. The results indicate that a significant number of participants found certain recommended learning materials to be engaging and surprising, providing evidence that our system has the potential to facilitate serendipitous learning experiences within informal learning contexts.
Synergistic Effects of BaTiO3 and MFe2O4 (M = Mn, Ni, Cu, Zn, and Co) Nanoparticles as Artificial Pinning Centers on the Performance of YBa2Cu3Oy Superconductor
Authors: Dair Amjad S. , Slimani Yassine , Hannachi Essia , Ben Azzouz Faten , Almessiere Munirah A.
Published: November 2024

Large-scale superconductor applications necessitate a superconducting matrix with pinning sites (PSs) that immobilize vortices at elevated temperatures and magnetic fields. While previous works focused on the single addition of nanoparticles, the simultaneous inclusion of different nanoparticles into a superconducting matrix can be an effective way to achieve an improved flux pinning capacity. The purpose of this study is to explore the influence of mixed-nanoparticle pinning, with the co-addition of non-magnetic (BaTiO3; BT) and various types of magnetic spinel ferrite (MFe2O4, abbreviated as MFO, where M = Mn, Co, Cu, Zn, and Ni) nanoparticles, on the superconductivity and flux pinning performances of the high-temperature superconductor YBa2Cu3Oy (YBCO). An analysis of X-Ray diffraction (XRD) data of BT–MFe2O4-co-added YBCO samples showed the formation of an orthorhombic structure with Pmmm symmetry. According to electrical resistivity measurements, the emergence of the superconducting state below Tcoffset (zero-resistivity temperature) was proven for all samples. The highest Tcoffset value was recorded for the Y-BT-MnFO sample, while the minimum value was obtained for the Y-BT-ZnFO sample. Direct current (DC) magnetization results showed good magnetic flux pinning performance for all the co-added samples compared to the pristine sample but with some discrepancies. At 77 K, the values of the self-critical current density (self-Jcm) and maximum pinning force (Fpmax) for the Y-BT-MnFO sample were found to be eight times higher and seventeen times greater than those for the pristine sample, respectively. The results acquired suggested that mixing the BT phase with an appropriate type of spinel ferrite nanoparticles can be a practical solution to the problem of degradation of the critical current density of the YBCO material.
Evaluating the Usability of a Remote Ischemic Conditioning Device for Pre-Hospital Stroke Management: Insights from Paramedic Simulations
Authors: Drozdowska Bogna Anna , Lam Kaden , Doolan Cody , Violato Efrem , Ganesh Aravind
Published: November 2024

Background/Objectives: In acute stroke, often-prolonged hospital transport times present an opportunity for early interventions to salvage brain tissue. Remote ischemic conditioning (RIC), where brief cycles of ischemia–reperfusion in a limb are induced to protect the brain, is a promising treatment for this setting. We assessed the usability of a novel RIC system in a simulated emergency response scenario. Methods: Paramedics were asked to use the RIC device in an emergency stroke care and ambulance transport simulation, overseen by a confederate. Feedback on device use was collected through questionnaires, including the System Usability Scale (SUS) and the NASA Task Load Index (NASA-TLX), and a semi-structured interview. Questionnaire responses were summarized using descriptive statistics; interview transcripts were analyzed thematically. Results: Nine paramedics (including the confederate) participated, with a mean of 10.0 ± 10.3 years of professional experience. Questionnaire responses indicated high device usability (mean SUS score: 85.3 ± 12.9 out of 100) and low task-related demands, effort, and frustration (mean NASA-TLX domain scores: ≤3.9 out of 20). Seven paramedics stated they would use the device in daily practice. They expressed concerns related to display screen clarity, interference with standard procedures, cable management, device fragility, and patient discomfort. Suggested improvements included adding indicators of device performance and refining the cuff design. Conclusions: While the device was considered easy to use, paramedics also identified important areas of improvement. With a small, localized study sample, our findings are primarily applicable to the refinement of the RICovery system for use in future clinical trials in the same healthcare setting. However, feedback on the importance of mitigating potential interference of newly introduced procedures with those already established, robustness of equipment, and effective paramedic–patient communication may also help inform the design of other pre-hospital interventions.
The Impact of Ageing on Episodic Memory Retrieval: How Valence Influences Neural Functional Connectivity
Authors: Constantinou Marianna , Pecchinenda Anna , Burianová Hana , Yankouskaya Ala
Published: November 2024

Age-related decline in episodic memory is often linked to structural and functional changes in the brain. Here, we investigated how these alterations might affect functional connectivity during memory retrieval following exposure to emotional stimuli. Using functional magnetic resonance imaging (fMRI), participants viewed images with varying emotional valences (positive, negative, and neutral) followed by unrelated non-arousing videos and were then asked to retrieve an episodic detail from the previously shown video. We conducted Multivariate Pattern Analysis (MVPA) to identify regions with divergent responses between age groups, which then served as seeds in Seed-Based Connectivity (SBC) analyses. The results revealed an age-related decline in behavioural performance following exposure to negative stimuli but preserved performance following positive stimuli. Young adults exhibited increased functional connectivity following negative valence. Conversely, old adults displayed increased connectivity more scarcely, and only following positive valence. These findings point to an adaptive response of the impact of emotions on task performance that depends on neural adaptations related to ageing. This suggests that age-related changes in functional connectivity might underlie how emotions influence memory, highlighting the need to tailor memory support strategies in older adulthood.
Quantifying Withanolides in Plasma: Pharmacokinetic Studies and Analytical Methods
Authors: Speers Alex B , Lozano-Ortiz Axel , Soumyanath Amala
Published: November 2024

Withania somnifera (common name: ashwagandha; WS) is an Ayurvedic botanical that has become popular for its reputed effects on stress and insomnia. Research into the bioactive compounds responsible for the biological effects of WS has largely focused on withanolides, a group of steroidal lactones commonly found in the Solanaceae family. Until recently, however, it was unclear which, if any, withanolides were present in the plasma after the ingestion of WS products. The aim of this review is to summarize current knowledge regarding the plasma pharmacokinetics of withanolides found in WS and the analytical methods developed to detect them in plasma. Twenty studies (sixteen animal, four human) were identified in which isolated withanolides or withanolide-containing products were administered to animals or humans and quantified in plasma. Withanolides were commonly analyzed using reversed-phase liquid chromatography coupled to mass spectrometry. Plasma concentrations of withanolides varied significantly depending on the substance administered, withanolide dose, and route of administration. Plasma pharmacokinetics of withaferin A, withanolide A, withanolide B, withanoside IV, 12-deoxywithastramonolide, and withanone have been reported in rodents (Cmax range: 5.6–8410 ng/mL), while withaferin A, withanolide A, 12-deoxywithastramonolide, and withanoside IV pharmacokinetic parameters have been described in humans (Cmax range: 0.1–49.5 ng/mL).
PAMK Ameliorates Non-Alcoholic Steatohepatitis and Associated Anxiety/Depression-like Behaviors Through Restoring Gut Microbiota and Metabolites in Mice
Authors: Yang Jianmei , Ou Wanyi , Lin Guiru , Wang Yuanfei , Chen Dongliang , Zeng Ze , Chen Zumin , Lu Xiaomin , Wu Aiping , Lin Chenli , Liang Yinji
Published: November 2024

Objectives: Long-term Western diet-induced non-alcoholic steatohepatitis (NASH) can lead to liver cirrhosis and NASH-associated hepatocellular carcinoma, which are end-stage liver diseases. Meanwhile, NASH is associated with mental burden and worsens as the disease progresses. Atractylodes Macrocephala Koidz (AMK) is one of the main ingredients of Shenling Baizhu San, and the effect of Polysaccharide from AMK ameliorates (PAMK), as an important medicinal ingredient of AMK, on NASH and associated anxiety/depression-like behaviors is still unclear. Methods: This study investigated the protective effect of PAMK on NASH and associated anxiety/depression-like behaviors through a Western diet-induced NASH mice model. Results: showed that PAMK decreased the concentrations of liver TC, TG, and serum AST and ALT, improving glucose tolerance, and reducing liver steatosis and fibrosis. Moreover, the expression of liver IL-6, IL-1β, TNF-α, IL-18 and MCP-1 could be reduced by PAMK significantly. Additionally, PAMK decreased anxiety/depression-like behaviors and expression of IL-6, IL-1β, TNF-α, and MCP-1 in the hippocampus. 16S rRNA gene sequencing revealed that PAMK diminished the Firmicutes/Bacteroidetes ratio and abundance of Faecalibaculum_rodentium, and increased the abundance of Muribaculaceae. This might be related to gene abundance of Pentose, the glucuronate interconversions pathway and carbohydrate enzymes (GH1, GH4). Serum metabolomics suggested that PC (18:5e/2:0), PC (16:2e/2:0), Lysopc 20:4, PC (16:0/2:0), and LPC 19:0 upregulated significantly after PAMK intervention, together with the enrichment of carbon metabolism and Citrate cycle pathways specially. Conclusions: PAMK as a potential prebiotic ameliorated NASH and associated anxiety/depression-like behaviors in mice, probably by regulating Faecalibaculum_rodentium, carbohydrate enzymes and lipid metabolites.
Associations Between Dietary Patterns and Quality of Life in a Longitudinal Cohort of Colorectal Cancer Survivors
Authors: Smith Kristen S. , Gudenkauf Lisa M. , Hoogland Aasha I. , Li Xiaoyin , Hoobler Rachel , Playdon Mary C. , Gigic Biljana , Small Brent J. , Gonzalez Brian D. , Oswald Laura B. , Byrd Doratha A. , Greathouse K. Leigh , Ulrich Cornelia M. , Li Christopher I. , Shibata David , Toriola Adetunji T. , Peoples Anita R. , Siegel Erin M. , Figueiredo Jane C. , Jim Heather S. L. , Crowder Sylvia L.
Published: November 2024

Purpose: To characterize dietary patterns and examine associations with cross-sectional and longitudinal changes in quality of life (QOL) over approximately one year after colorectal cancer (CRC) diagnosis. Methods: The ColoCare Study is an international, multi-center, prospective cohort study of newly diagnosed CRC survivors of any stage. A subset of participants with CRC in the United States completed patient-reported outcome measures at 6- and 12-months post-enrollment, including the Food Frequency Questionnaire (FFQ) and European Organization for the Research and Treatment of Cancer Quality of Life Questionnaire (EORTC QLQ-C30). Dietary patterns at 6 months (around the time of treatment completion) were identified using Principal Component Analysis (PCA) with varimax rotation. Adherence scores were calculated for participants within each dietary pattern, with higher scores indicating higher adherence. Mixed models were used to examine the effect of each dietary pattern on changes in QOL at 6- and 12-month follow-ups, controlling for cancer stage, biological sex, body mass index (BMI), smoking status, and age. Results: Participants (N = 174) were, on average, 56 ± 14 years old and were mostly female (51.5%), stage III or IV (51.7%), never smokers (60.2%), non-Hispanic (97.1%), and White (83.3%) with a BMI of 27.9 ± 6.1 kg/m2. PCA revealed two emerging dietary patterns: “Western diet”, characterized by processed meats, refined grains, and sugars, and “Prudent diet” characterized by lean proteins, fruits, and vegetables. Higher adherence to a Western diet was associated with worse social functioning at 6-month follow-up (FE = −12.6, p = 0.010). Loss of appetite from 6 to 12 months was associated with higher adherence to both the Western and Prudent dietary patterns (FE = 1.5, p = 0.044; FE = 1.3, p = 0.046, respectively). Neither dietary pattern was associated with global QOL score at 6- or 12-month follow-up (p’s > 0.05). Conclusions: Among CRC survivors in the United States, the Western diet was concurrently associated with worse social functioning. Loss of appetite was reported by CRC survivors following both dietary patterns, suggesting that loss of appetite may be a global experience for CRC survivors during this timeframe. Further research is needed to understand specific social challenges experienced by CRC survivors and develop supportive care interventions to address appetite and nutritional concerns.
Evaluation of the Staff Educational Components of the PROMOTE Program to Improve Resident Hydration
Authors: Keller Heather H. , Aravind Raksha , Devlin Kristina , Syed Safura , Werden Abrams Sophia , Lengyel Christina , Yoon Minn N. , Namasivayam-MacDonald Ashwini , Slaughter Susan E. , Gaspar Phyllis , Liu Wen
Published: November 2024

Background/Objectives: Inadequate fluid intake is prevalent among older adults living in care settings and can lead to dehydration-related events such as falls and hospitalization. Staff knowledge and confidence using diverse strategies is needed to provide adequate hydration to residents. PROMOTE is a multicomponent intervention designed to support staff to increase resident fluid intake between meals. This study evaluated the educational components of PROMOTE. Methods: Participants (n = 87) working in long-term care or retirement homes completed an online pre-/post-test evaluation of a 7 min educational video. Key informant participants (n = 13) reviewed all educational materials, evaluated their usefulness and feasibility, and were interviewed to identify how to improve the materials. Results: The educational video improved knowledge (e.g., self-rating of knowledge pre-test median 8, standard error of the mean (SEM) 0.18; post-test median 9, SEM 0.13) and confidence. Participants intended to use PROMOTE strategies in their work with residents (1 [very likely] to 10 [very unlikely] median 2.0 SEM 0.27). Key informants rated the hydration of residents as an organizational priority (median 9.0 SEM 0.42) and all indicated that they would use the educational video in their future training. Less feasible educational components as rated by key informants included huddle discussions and email pushes. Posters were seen as feasible (54%) but only somewhat useful (77%). Conclusions: Brief educational videos can improve staff knowledge and confidence regarding providing adequate hydration to residents. Having several educational components that can be used with this video was viewed positively. Recommendations were made to improve the materials.
Dietary Habits, Nutritional Knowledge, and Their Impact on Thyroid Health in Women: A Cross-Sectional Study
Authors: Gwizdak Przemysław , Szlacheta Patryk , Łaskawiec-Żuławińska Daria , Grajek Mateusz , Krupa-Kotara Karolina , Markowski Jarosław , Korzonek-Szlacheta Ilona
Published: November 2024

Background: The thyroid gland plays a crucial role in regulating metabolism and various bodily functions through hormone production. Women are particularly susceptible to thyroid disorders such as hypothyroidism and Hashimoto’s disease, with associated symptoms affecting overall well-being. Prior research has inadequately addressed the influence of dietary habits and nutritional knowledge on thyroid health, especially in women. Objective: This study aimed to evaluate the dietary habits and nutritional awareness of women aged 18–45 with diagnosed thyroid disorders, emphasizing the effects of education level on knowledge and dietary practices. Material and Methods: A cross-sectional survey was conducted with 297 women diagnosed with thyroid conditions. The survey assessed demographics, comorbidities, hydration habits, and knowledge about nutrient intake critical for thyroid health. Chi-square tests, ANOVA, and correlation analyses were performed to evaluate associations. Results: Hypothyroidism and Hashimoto’s disease were most prevalent among younger women (18–25 years). A significant association was observed between higher education and knowledge of protein and carbohydrate roles in managing thyroid health (p < 0.01). Women with higher educational backgrounds more frequently used healthier cooking methods and were more informed about beneficial nutrients, including vitamin D and omega-3. A chi-square test indicated that low water intake was significantly associated with comorbid conditions, including insulin resistance and cardiovascular disease (p < 0.01). Conclusions: Significant gaps remain in dietary knowledge, particularly concerning protein intake and nutrient–drug interactions, indicating a need for targeted dietary education. Women with higher education demonstrated greater dietary awareness, emphasizing the importance of tailored educational interventions to enhance thyroid disorder management.
Effect of the Intake of Solid Block Dairy Products Like Cheese on Serum Uric Acid in Children: A Preliminary Mechanistic Investigation
Authors: Lu Zhongting , Tang Zhenchuang , Guo Xin , Liu Lei , Cheng Xuemei , Yu Lianlong , Cheng Guangyan
Published: November 2024

Objective: This study aimed to investigate the relationship between the intake of solid block dairy products like cheese and serum uric acid levels, along with its potential physiological mechanisms. Methods: Data for our study were obtained from the Chinese Children and Lactating Women Nutrition and Health Surveillance. Generalized linear models and restricted cubic splines were employed to analyze the relationship between the intake of solid block dairy products like cheese and serum uric acid levels. Two-sample Mendelian randomization (TSMR) analysis was conducted to infer causality, based on a large sample size and robust methodology. Gene Ontology (GO) enrichment analysis was also performed to identify potential biological pathways. Results: Among all types of dairy products, a significant negative association with serum uric acid levels was observed only for the intake of solid block dairy products like cheese, regardless of covariate adjustment (β = −0.182, p < 0.001). TSMR results supported a negative causal relationship between cheese intake and serum uric acid levels (β = −0.103, 95% CI: −0.149 to −0.057; p = 0.002). The JAK-STAT signaling pathway and autophagy regulation were identified as potential physiological mechanisms underlying this relationship. Conclusions: The intake of solid block dairy products like cheese was found to result in decreased levels of serum uric acid, with potential mechanisms involving the JAK-STAT signaling pathway and the regulation of autophagy.
Quality of Life Among Natural Menopausal Women and Early Surgical Menopausal Women: A Study from Greece
Authors: Kavga Fotini , Bothou Anastasia , Nanou Christina , Kyrkou Giannoula , Vivilaki Victoria , Deltsidou Anna
Published: November 2024

Background/Objectives: The general health and well-being of middle-aged women have become a major public health issue worldwide. More than 80% of women experience physical or psychological symptoms during the transition to menopause. This study aims to compare the effect of menopause on quality of life (QOL) in two groups of women undergoing natural and surgical menopause. Methods: The sample consisted of 100 female patients from a Greek hospital in Athens, with an average age of 44.5 years, half of whom had natural menopause, while the remaining women had iatrogenic menopause after surgery for any reason other than malignancy. A questionnaire related to the QOL in menopause was used to collect the data. The scale used to evaluate the QOL of women is the Utian QOL Scale (UQOL), translated into Greek. Results: From the analysis of the data, it was found that there is no statistically significant difference between the QOL of women with natural and surgical menopause. Menopausal symptoms, psychosocial and sexual health, as well as the general health of the two groups, showed similar rates (OR: 63.7–66.6, p = 0.248). The only statistically significant difference found was in weight gain, with natural menopausal women having greater weight gain compared to surgically menopausal women (p = 0.041). Conclusions: Menopausal symptoms are associated with a decrease in women’s QOL. However, QOL is affected regardless of the type of menopause transition. This study was not registered.
Influence of Knowledge and Cultural Beliefs on Attitudes Toward HPV Vaccination Among Israeli Nurses and Nursing Students: Implications for Vaccine Advocacy
Authors: Gendler Yulia , Ben-Aroya Nurit , Blau Ayala
Published: November 2024

Background: Human papillomavirus (HPV) poses a significant health burden, yet the vaccine which successfully prevents HPV and its associated cancers remains underutilized. Nurses play a crucial role in patient education and advocacy for HPV vaccination. This study explores how knowledge, along with cultural, religious, and social beliefs, shapes the attitudes of Israeli nurses and nursing students toward the HPV vaccine and their advocacy efforts. Methods: A cross-sectional study was conducted from August 2023 to January 2024 using an anonymous online questionnaire distributed via Facebook groups targeting Israeli nurses and nursing students. The questionnaire assessed demographic data, HPV-related knowledge, and attitudes toward HPV vaccination. Results: The study included 458 participants (229 nurses and 229 nursing students). Significant knowledge gaps were found, with 52% of participants incorrectly believing that the vaccine can cure existing infections and 47% mistakenly believing that it is administered in a single dose. Logistic regression revealed that nurses working in community settings were more likely to have positive attitudes toward HPV vaccination (OR = 2.98, 95% CI: 1.84–4.85). Higher levels of HPV-related knowledge (OR = 3.35, 95% CI: 2.10–5.35) and secular or traditional religious affiliations (OR = 2.45, 95% CI: 1.52–3.97) were strongly associated with positive attitudes toward and advocacy for the vaccine. Conclusions: Targeted educational programs addressing knowledge gaps, especially those tailored to Israel’s cultural and religious diversity, are crucial for empowering nurses and nursing students as advocates for HPV vaccination. Enhancing their understanding of HPV can increase vaccine uptake, reduce the incidence of HPV-related diseases, and strengthen public health initiatives in Israel.
Factors Associated with and Predictive Model for Resilience in Family Caregivers of Care-Dependent Adults
Authors: Gutierrez-Baena Belen , Gilart Ester , Romero-Grimaldi Carmen
Published: November 2024

Background: Caring for dependent people is an intense task that leads family caregivers to suffer physical or mental pathologies. Resilience is a protective factor that makes an individual more resistant to adverse events. Some characteristics of the caregiver or the care provided predispose the caregiver to be less resilient. Knowing these characteristics will allow us to detect vulnerable caregivers. Aim: The aim of this study was to explore the factors associated with caregiver resilience and establish a predictive model, including the relationship between preparedness, burden, resilience, and anxiety. Materials and Methods: The study design was descriptive, observational, and cross-sectional, with purposive sampling. The sample included 172 family caregivers of care-dependent patients in Spain. Questionnaires were administered to assess caregiver resilience, anxiety, burden, and the preparedness of care-dependent patients. Correlation, univariate, and multiple linear regression analyses were performed to identify the factors associated with resilience. Results: We found that there is a correlation between resilience, preparation, and burden. Factors related to resilience include age, the caregiver’s health status, kinship, childcare, and state aid granted. Caregiver preparedness is the factor that most predicts caregiver resilience in our model. Caregivers with high levels of resilience also demonstrated high levels of preparedness and experienced less burden than those with low levels of resilience. Conclusions: These findings emphasize the relevance of improving the preparation of family caregivers to increase their resilience and, at the same time, the quality of care provided.
On the Thermomechanics of Hadrons and Their Mass Spectrum
Authors: Chiatti Leonardo
Published: November 2024

A little-known thermomechanical relation between entropy and action, originally discovered by Boltzmann in the classical domain, was later reconsidered by de Broglie in relation to the wave–particle duality in the free propagation of single particles. In this paper, we present a version adapted to the phenomenological description of the hadronization process. The substantial difference with respect to the original de Broglie scheme is represented by the universality of the temperature at which the process occurs; this, in fact, coincides with the Hagedorn temperature. The main results are as follows: (1) a clear connection between the universality of the temperature and the existence of a confinement radius of the color forces; (2) a lower bound on the hadronic mass, represented by the universal temperature, in agreement with experimental data; and (3) a scale invariance, which allows the reproduction of the well-known hadronic mass spectrum solution of the statistical bootstrap model. The approach therefore presents a heuristic interest connected to the study of the strong interaction.
Observational Study on the Clinical Reality of Community-Acquired Respiratory Virus Infections in Adults and Older Individuals
Authors: Nagasawa Masayuki , Udagawa Tomohiro , Kato Tomoyuki , Tanaka Ippei , Yamamoto Ren , Sakaguchi Hayato , Sekikawa Yoshiyuki
Published: November 2024

The impact of common respiratory virus infections on adults and older individuals in the community is unclear, excluding seasonal influenza viruses. We examined FilmArray® tests performed on 1828 children aged <10 years and 10,803 adults, including cases with few respiratory symptoms, between January 2021 and June 2024. Approximately 80% of the children tested positive for ≥1 viruses, while 9.5% of the adults tested positive mostly for severe acute respiratory syndrome corona virus-2 (SARS-CoV-2). Besides SARS-CoV-2 infection, 66 out of 97 patients (68.0%) aged >60 years with rhinovirus/enterovirus (RV/EV), respiratory syncytial virus (RSV), parainfluenza virus-3 (PIV-3), or human metapneumovirus (hMPV) infection required hospitalization, of whom seven died; 26 out of 160 patients (16.3%) aged <60 years required hospitalization mostly because of deterioration of bronchial asthma, with no reported deaths. In older patients with RV/EV infection, three with few respiratory symptoms died due to worsened heart failure. Although the frequency of common respiratory virus infections in older adults is low, it may be overlooked because of subclinical respiratory symptoms, and its clinical significance in worsening comorbidities in older adults should not be underestimated.