bluuebunny/crossref_metadata_2025 huggingface dataset:
Accuracy and Reliability in the Simulation of Vibrational Spectra: A Comprehensive Benchmark of Energies and Intensities Issuing From Generalized Vibrational Perturbation Theory to Second Order (GVPT2)
Authors: Yang Qin , Mendolicchio Marco , Barone Vincenzo , Bloino Julien
Published: May 2021

Vibrational spectroscopy represents an active frontier for the identification and characterization of molecular species in the context of astrochemistry and astrobiology. As new missions will provide more data over broader ranges and at higher resolution, especially in the infrared region, which could be complemented with new spectrometers in the future, support from laboratory experiments and theory is crucial. In particular, computational spectroscopy is playing an increasing role in deepening our understanding of the origin and nature of the observed bands in extreme conditions characterizing the interstellar medium or some planetary atmospheres, not easily reproducible on Earth. In this connection, the best compromise between reliability, feasibility and ease of interpretation is still a matter of concern due to the interplay of several factors in determining the final spectral outcome, with larger molecular systems and non-covalent complexes further exacerbating the dichotomy between accuracy and computational cost. In this context, second-order vibrational perturbation theory (VPT2) together with density functional theory (DFT) has become particularly appealing. The well-known problem of the reliability of exchange-correlation functionals, coupled with the treatment of resonances in VPT2, represents a challenge for the determination of standardized or “black-box” protocols, despite successful examples in the literature. With the aim of getting a clear picture of the achievable accuracy and reliability of DFT-based VPT2 calculations, a multi-step study will be carried out here. Beyond the definition of the functional, the impact of the basis set and the influence of the resonance treatment in VPT2 will be analyzed. For a better understanding of the computational aspects and the results, a short summary of vibrational perturbation theory and the overall treatment of resonances for both energies and intensities will be given. The first part of the benchmark will focus on small molecules, for which very accurate experimental and theoretical data are available, to investigate electronic structure calculation methods. Beyond the reliability of energies, widely used for such systems, the issue of intensities will also be investigated in detail. The best performing electronic structure methods will then be used to treat larger molecular systems, with more complex topologies and resonance patterns.
Intentions of Environmentally Friendly Behavior Among Sports Club Members: An Empirical Test of the Theory of Planned Behavior Across Genders and Sports
Authors: Braksiek Michael , Thormann Tim F. , Wicker Pamela
Published: May 2021

Environmentally friendly behavior has become increasingly important in recent years to reduce the speed of climate change and its negative impacts. Individual behavior, including environmentally friendly behavior, is largely formed by behavioral intentions. This study draws on the theory of planned behavior to examine the effects of attitudes toward the behavior, subjective norms, and perceived behavioral control on intentions of environmentally friendly behavior. It also investigates differences between genders and among sports. The study is based on data from a nationwide online survey of community sports club members in Germany in five team/racket sports (n= 3,036). Existing measures to operationalize the constructs were adapted to the present research context. The data were analyzed using structural equation modeling. The results show that the theoretical assumptions of the theory of planned behavior were largely supported by the data, implying that the antecedents of environmentally friendly behavioral intentions can be applied to club members. Furthermore, gender- and sports-specific differences in the antecedents–intention relationship were detected. This study is among the first to examine environmentally friendly behavioral intentions in community sports clubs. It adds to an increasing body of research investigating environmental sustainability in sports.
A Research Road Map for Responsible Use of Agricultural Nitrogen
Authors: Udvardi Michael , Below Frederick E. , Castellano Michael J. , Eagle Alison J. , Giller Ken E. , Ladha Jagdish Kumar , Liu Xuejun , Maaz Tai McClellan , Nova-Franco Barbara , Raghuram Nandula , Robertson G. Philip , Roy Sonali , Saha Malay , Schmidt Susanne , Tegeder Mechthild , York Larry M. , Peters John W.
Published: May 2021

Nitrogen (N) is an essential but generally limiting nutrient for biological systems. Development of the Haber-Bosch industrial process for ammonia synthesis helped to relieve N limitation of agricultural production, fueling the Green Revolution and reducing hunger. However, the massive use of industrial N fertilizer has doubled the N moving through the global N cycle with dramatic environmental consequences that threaten planetary health. Thus, there is an urgent need to reduce losses of reactive N from agriculture, while ensuring sufficient N inputs for food security. Here we review current knowledge related to N use efficiency (NUE) in agriculture and identify research opportunities in the areas of agronomy, plant breeding, biological N fixation (BNF), soil N cycling, and modeling to achieve responsible, sustainable use of N in agriculture. Amongst these opportunities, improved agricultural practices that synchronize crop N demand with soil N availability are low-hanging fruit. Crop breeding that targets root and shoot physiological processes will likely increase N uptake and utilization of soil N, while breeding for BNF effectiveness in legumes will enhance overall system NUE. Likewise, engineering of novel N-fixing symbioses in non-legumes could reduce the need for chemical fertilizers in agroecosystems but is a much longer-term goal. The use of simulation modeling to conceptualize the complex, interwoven processes that affect agroecosystem NUE, along with multi-objective optimization, will also accelerate NUE gains.
Case Report: Optimizing Pre- and Intraoperative Planning With Hyperaccuracy Three-Dimensional Virtual Models for a Challenging Case of Robotic Partial Nephrectomy for Two Complex Renal Masses in a Horseshoe Kidney
Authors: Campi Riccardo , Sessa Francesco , Rivetti Anna , Pecoraro Alessio , Barzaghi Paolo , Morselli Simone , Polverino Paolo , Nicoletti Rossella , Li Marzi Vincenzo , Spatafora Pietro , Sebastianelli Arcangelo , Gacci Mauro , Vignolini Graziano , Serni Sergio
Published: May 2021

Objective: To report a case of robot-assisted partial nephrectomy (RAPN) for two highly complex renal tumors in a patient with a Horseshoe kidney (HSK), focusing on the utility of hyperaccuracy three-dimensional (HA3D) virtual models for accurate preoperative and intraoperative planning of the procedure.

Methods: A 74-year-old Caucasian male patient was referred to our Unit for incidental detection of two complex renal masses in the left portion of a HSK. The 50 × 55 mm, larger, predominantly exophytic renal mass was located at the middle-lower pole of the left-sided kidney (PADUA score 9). The 16 × 17 mm, smaller, hilar renal mass was located at the middle-higher pole of the left-sided kidney (PADUA score 9). Contrast-enhanced CT scan images in DICOM format were processed using a dedicated software to achieve a HA3D virtual reconstructions. RAPN was performed by a highly experienced surgeon using the da Vinci Si robotic platform with a three-arm configuration. A selective delayed clamping strategy was adopted for resection of the larger renal mass while a clampless strategy was adopted for the smaller renal mass. An enucleative resection strategy was pursued for both tumors.

Results: The overall operative time was 150 min, with a warm ischemia time of 21 min. No intraoperative or postoperative complications were recorded. Final resection technique according to the SIB score was pure enucleation for both masses. At histopathological analysis, both renal masses were clear cell renal cell carcinoma (ccRCC) (stage pT1bNxMx and pT3aNxMx for the larger and smaller mass, respectively). At a follow-up of 7 months, there was no evidence of local or systemic recurrence.

Conclusions: Surgical management of complex renal masses in patients with HSKs is challenging and decision-making is highly nuanced. To optimize postoperative outcomes, proper surgical experience and careful preoperative planning are key. In this regard, 3D models can play a crucial role to refine patient counseling, surgical decision-making, and pre- and intraoperative planning during RAPN, tailoring surgical strategies and techniques according to the single patient's anatomy.
En Bloc Resection of Bladder Tumor—Is It the Way Forward?
Authors: Fankhauser Christian Daniel , Wettstein Marian Severin , Afferi Luca , Grossmann Nico Christian , Mostafid Hugh
Published: May 2021

Transurethral resection of bladder tumors (TURBT) represents the cornerstone in diagnosis and treatment of bladder cancer but recurrence is observed in up to 80% and over- or understaging with TURBT is common. A more recent development to overcome these limitations represents en-bloc resection of bladder tumors (ERBT) which offers several advantages over TURBT. In this report, we briefly review studies assessing outcomes of bladder cancer patients undergoing ERBT. Most randomized and non-randomized trial demonstrate improvement in clinical outcomes for ERBT over TURBT, however more pathological and translational studies are warranted.
Pan-Genome Analysis of Vibrio cholerae and Vibrio metschnikovii Strains Isolated From Migratory Birds at Dali Nouer Lake in Chifeng, China
Authors: Zheng Lin , Zhu Ling-Wei , Jing Jie , Guan Jia-yao , Lu Ge-Jin , Xie Lin-Hong , Ji Xue , Chu Dong , Sun Yang , Chen Ping , Guo Xue-Jun
Published: May 2021

Migratory birds are recently recognized as Vibrio disease vectors, but may be widespread transporters of Vibrio strains. We isolated Vibrio cholerae (V. cholerae) and Vibrio metschnikovii (V. metschnikovii) strains from migratory bird epidemic samples from 2017 to 2018 and isolated V. metschnikovii from migratory bird feces in 2019 from bird samples taken from the Inner Mongolia autonomous region of China. To investigate the evolution of these two Vibrio species, we sequenced the genomes of 40 V. cholerae strains and 34 V. metschnikovii strains isolated from the bird samples and compared these genomes with reference strain genomes. The pan-genome of all V. cholerae and V. metschnikovii genomes was large, with strains exhibiting considerable individual differences. A total of 2,130 and 1,352 core genes were identified in the V. cholerae and V. metschnikovii genomes, respectively, while dispensable genes accounted for 16,180 and 9,178 of all genes for the two strains, respectively. All V. cholerae strains isolated from the migratory birds that encoded T6SS and hlyA were non-O1/O139 serotypes without the ability to produce CTX. These strains also lacked the ability to produce the TCP fimbriae nor the extracellular matrix protein RbmA and could not metabolize trimetlylamine oxide (TMAO). Thus, these characteristics render them unlikely to be pandemic-inducing strains. However, a V. metschnikovii isolate encoding the complete T6SS system was isolated for the first time. These data provide new molecular insights into the diversity of V. cholerae and V. metschnikovii isolates recovered from migratory birds.
Feedback Linearization of Inertially Actuated Jumping Robots
Authors: Cox Adam , Razzaghi Pouria , Hurmuzlu Yildirim
Published: May 2021

Inertially Actuated Jumping Robots (IAJR) provide a promising new means of locomotion. The difficulty of IAJR is found in the hybrid nature of the ground contact/flying dynamics. Recent research studies in our Systems Lab have provided a family tree of inertially actuated locomotion systems. The proposed Tapping Robot is the most prompt member of this tree. In this paper, a feedback linearization controller is introduced to provide controllability given the 3-dimensional motion complexity. The research objective is to create a general controller that can regulate the locomotion of Inertially Actuated Jumping Robots. The expected results can specify a desired speed and/or jump height, and the controller ensures the desired values are achieved. The controller can achieve the greatest response for the Basketball Robot at a maximum jump height of 0.25 m, which is greater than the former performance with approximately 0.18 m. The design paradigm used on the Basketball Robot was extended to the Tapping Robot. The Tapping Robot achieved a stable average forward velocity of 0.0773 m/s in simulation and 0.157 m/s in experimental results, which is faster than the forward velocity of former robot, Pony III, with 0.045 m/s.
Foliar Application of Zn Agrichemicals Affects the Bioavailability of Arsenic, Cadmium and Micronutrients to Rice (Oryza sativa L.) in Flooded Paddy Soil
Authors: Sharifan Hamidreza , Ma Xingmao
Published: May 2021

Zinc (Zn) is a key micronutrient affecting bio-functions of crops. Impacts of foliar application of three forms of Zn-containing agrichemicals on the physiology of rice seedlings and their uptake of heavy metal(loid)s and micronutrients from fully flooded paddy soils were investigated in a greenhouse study. Rice seedlings 45 days after germination were randomly exposed to an equivalent amount of 100 mg L−1 of zinc oxide nanoparticles (ZnONPs), zinc oxide bulk particles (ZnOBPs), and zinc salts (Zn2+) through foliar exposure. At termination, rice seedlings were divided into roots and shoots and the concerned metal contents in plant tissues were determined with an inductively coupled plasma mass spectrometry (ICP-MS). Our results demonstrated that all Zn agrichemicals displayed a similar effect on plant growth, but ZnONPs led to the greatest root exudate excretion and greatest inhibition in arsenic (As) uptake. Foliar application of ZnONPs resulted in 28% less As in rice shoots while Zn2+ only caused a 15% decrease in As accumulation in the same tissues. ZnOBPs had minimal impact on As concentration in rice shoots. ZnONPs also promoted iron (Fe) and copper (Cu) uptake in rice shoots, both of which are essential micronutrients for humans. The results suggest that foliar application of ZnONPs resulted in more favorable outcomes for plant growth and food safety than other two types of Zn. Overall, our results showed that application of nanoagrichemicals have important food safety and nutrition implications in addition to their agronomical roles and ZnONPs could be potentially used to alleviate As uptake and fortify Zn and other essential micronutrients in rice grains.
QTL Mapping of Seedling and Adult Plant Resistance to Septoria Tritici Blotch in Winter Wheat cv. Mandub (Triticum aestivum L.)
Authors: Piaskowska Dominika , Piechota Urszula , Radecka-Janusik Magdalena , Czembor Paweł
Published: May 2021

Septoria tritici blotch (STB) is one of the most devastating foliar diseases of wheat worldwide. Host resistance is the most economical and safest method of controlling the disease, and information on resistance loci is crucial for effective breeding for resistance programs. In this study we used a mapping population consisting of 126 doubled-haploid lines developed from a cross between the resistant cultivar Mandub and the susceptible cultivar Begra. Three monopycnidiospore isolates of Z. tritici with diverse pathogenicity were used to test the mapping population and parents’ STB resistance at the seedling stage (under a controlled environment) and adult plant stage (polytunnel). For both types of environments, the percentage leaf area covered by necrosis (NEC) and pycnidia (PYC) was determined. A linkage map comprising 5899 DArTSNP and silicoDArT markers was used for the quantitative trait loci (QTL) analysis. The analysis showed five resistance loci on chromosomes 1B, 2B and 5B, four of which were derived from cv. Mandub. The location of QTL detected in our study on chromosomes 1B and 5B may suggest a possible identity or close linkage with Stb2/Stb11/StbWW and Stb1 loci, respectively. QStb.ihar-2B.4 and QStb.ihar-2B.5 detected on chromosome 2B do not co-localize with any known Stb genes. QStb.ihar-2B.4 seems to be a new resistance locus with a moderate effect (explaining 29.3% of NEC and 31.4% of PYC), conferring resistance at the seedling stage. The phenotypic variance explained by QTL detected in cv. Mandub ranged from 11.9% to 70.0%, thus proving that it is a good STB resistance source and can potentially be utilized in breeding programs.
The Regional Efficiency in the Use of European Agricultural Funds in Spain: Growth and Employment Analyses
Authors: Blanco Miguel , Ferasso Marcos , Bares Lydia
Published: May 2021

After World War II, the European Union launched the Common Agricultural Policy. Since then, this programme has been adapting to the new economic and social realities that the European agricultural sector has been going through. Currently, a large part of this programme has been financed with the European Agricultural Guarantee Funds and the European Agricultural Fund for Rural Development. Two packages of funds have been approved in recent decades: one for the 2007–2013 and another for the 2014–2020 periods. This research has had as its main objective to determine whether the Spanish regions maintain homogeneous levels of efficiency in the use of these resources in the management of agricultural programmes from a growth and employment perspective. A methodology that is frequently used by researchers in the efficiency analyses has been chosen: data envelopment analysis. Among the main conclusions obtained are that the efficiency in the management of agricultural funds is very uneven among the studied Spanish regions. Furthermore, these differences are maintained throughout the analysed periods. This study suggests changes and proposes criteria for the allocation of European resources to finance the projects presented by the Spanish regions.
Nutritional and Sensory Quality of Two Types of Cress Microgreens Depending on the Mineral Nutrition
Authors: Keutgen Norbert , Hausknecht Michael , Tomaszewska-Sowa Magda , Keutgen Anna Jadwiga
Published: May 2021

The present study addressed the combination of nutritional and sensory quality assessment of radish and garden cress as microgreens cultivated in different amounts of mineral nutrients under conditions that can be realized in a private household. The content of value adding compounds was determined by means of chemical analyses. Total flavor impression and visual appearance were rated by untrained consumer tests. In cotyledons of radish cress (Raphanus sativus L.), carotenoid, total phenols, nitrate contents, and antioxidant capacity decreased significantly with decreasing mineral content in the nutrient solution, whereas, in stems, total phenols and anthocyanin contents rose and nitrate content decreased significantly with decreasing mineral content. In garden cress (Lepidium sativum L.), carotenoid and nitrate contents decreased and anthocyanin content increased significantly with decreasing mineral content, indicating that the response of value adding compounds to changing amounts of minerals in the nutrient solution depends on the compound of interest, plant species, and even the plant organ of a species. The sensory quality of the studied microgreens was generally rated highest when mineral content in the nutrient solution was highest, indicating that sensory quality is not necessarily identical with nutritional quality. Considering the common practice in private households, cultivation with tap water represents an attractive compromise for nutritional and sensory quality in case of garden cress, whereas, for radish cress, the application of 25% modified Hoagland solution is recommended.
Effect of Nutrient Solution pH on the Growth, Yield and Quality of Taraxacum officinale and Reichardia picroides in a Floating Hydroponic System
Authors: Alexopoulos Alexios A. , Marandos Efstathios , Assimakopoulou Anna , Vidalis Nikolina , Petropoulos Spyridon A. , Karapanos Ioannis C.
Published: May 2021

Given the important medicinal and nutritional value of wild edible greens, the last few years there is an increasing interest for their domestication and commercial exploitation. However, information concerning their adaptation to environmental conditions and their response to modern agricultural systems are scarce. In the present study, the effect of nutrient solution pH (4.0, 5.5 and 7.0) on the growth, chemical composition and inorganic nutrition of Taraxacum officinale and Reichardia picroides plants grown indoors in a floating hydroponic system was evaluated. Both species performed better at pH 5.5 and were slightly affected by pH 7.0, whereas pH 4.0 was not prohibitive for growth for both species, although R. picroides was less tolerant than T. officinale at low pH. Moreover, pH 4.0 did not severely affect nutrients uptake and transport within the plant tissues, suggesting that R. picroides susceptibility to low pH should not be attributed to nutrients imbalance. Nevertheless, low pH positively enhanced the content in total soluble solids, total phenolics, chlorophylls (a, b and total) and carotenoids, and decreased nitrates in both species. In conclusion, the studied species could be successfully grown in soilless systems with nutrient solutions of varied pH. Moreover, low pH levels (pH = 4.0) seemed to be beneficial to nutritional and dietary value in both species highlighting the potential of commercial cultivation under adverse conditions, especially in sustainable farming systems.
Genomic Prediction and Genotype-by-Environment Interaction Analysis of Crown and Stem Rust in Ryegrasses in European Multi-Site Trials
Authors: Fois Mattia , Malinowska Marta , Schubiger Franz Xaver , Asp Torben
Published: May 2021

Climate change calls for novel approaches to include environmental effects in future breeding programs for forage crops. A set of ryegrasses (Lolium) varieties was evaluated in multiple European environments for crown rust (Puccinia coronata f. sp. lolii) and stem rust (P. graminis f. sp. graminicola) resistance. Additive Main Effect and Multiplicative Interaction (AMMI) analysis revealed significant genotype (G) and environment (E) effects as well as the interaction of both factors (G × E). Genotypes plus Genotype-by-Environment interaction (GGE) analysis grouped the tested environments in multiple mega-environments for both traits suggesting the presence of an environmental effect on the ryegrasses performances. The best performing varieties in the given mega-environments showed high resistance to crown as well as stem rust, and overall, tetraploid varieties performed better than diploid. Furthermore, we modeled G × E using a marker x environment interaction (M × E) model to predict the performance of varieties tested in some years but not in others. Our results showed that despite the limited number of varieties, the high number of observations allowed us to predict both traits’ performances with high accuracy. The results showed that genomic prediction using multi environmental trials could enhance breeding programs for the crown and stem rust in ryegrasses.
TeamMate: A Longitudinal Study of New Zealand Working Farm Dogs. III. Factors Affecting the Risk of Dogs Being Lost from the Workforce
Authors: Isaksen Katja E. , Linney Lori , Williamson Helen , Norman Elizabeth J. , Cave Nick J. , Cogger Naomi
Published: May 2021

Working farm dogs are essential to many livestock farmers. Little is known about factors that influence dogs’ risk of being lost from work. This paper explores risk factors for farm dogs being lost through death, euthanasia and retirement. All enrolled dogs were working and a minimum of 18 months old. Five data collection rounds were performed over four years. Data about dogs were collected from owners and dogs were given physical examinations by veterinarians. Dogs that were lost from work were counted and owner-reported reasons for loss were recorded. Multivariable logistic regression modelling was used to investigate risk factors for loss. Of 589 dogs, 81 were lost from work. Of these, 59 dogs died or were euthanized and 22 were retired. Farm dogs tended to reach advanced ages, with 38% being 10 years or older when last examined. Acute injury or illness was the most commonly owner-reported reason for loss. Age group (p < 0.0001) and lameness (p = 0.04, OR = 1.8) significantly affected dogs’ risk of being lost. These results expand our knowledge about factors that affect health, welfare and work in farm dogs. Further investigation into reasons for lameness may help improve health and welfare in working farm dogs.
Protective Effects of α-Lipoic Acid and Chlorogenic Acid on Cadmium-Induced Liver Injury in Three-Yellow Chickens
Authors: Shi Jiabin , Chang Xiaocui , Zou Hui , Gu Jianhong , Yuan Yan , Liu Xuezhong , Liu Zongping , Bian Jianchun
Published: May 2021

Cadmium (Cd) is a type of noxious heavy metal that is distributed widely. It can severely injure the hepatocytes and cause liver dysfunction by inducing oxidative stress and mitochondrial damage. We evaluated the protective effects of α-lipoic acid (α-LA) or chlorogenic acid (CGA) and their combination on counteracting cadmium toxicity in vivo in three-yellow chickens. For three months, CdCl2 (50 mg/L) was administrated through their drinking water, α-LA (400 mg/kg) was added to feed and CGA (45 mg/kg) was employed by gavage. The administration of Cd led to variations in growth performance, biochemical markers (of the liver, kidney and heart), hematological parameters, liver histopathology (which suggested hepatic injury) and ultrastructure of hepatocytes. Some antioxidant enzymes and oxidative stress parameters showed significant differences in the Cd-exposure group when compared with the control group. The groups treated with Cd and administrated α-LA or CGA showed significant amelioration with inhibited mitochondrial pathway-induced apoptosis. Combining both drugs was the most effective in reducing Cd toxicity in the liver. In summary, the results demonstrated that α-LA and CGA may be beneficial in alleviating oxidative stress induced by oxygen free radicals and tissue injury resulting from Cd-triggered hepatotoxicity.
Glucosamine and Chondroitin Sulfate: Is There Any Scientific Evidence for Their Effectiveness as Disease-Modifying Drugs in Knee Osteoarthritis Preclinical Studies?—A Systematic Review from 2000 to 2021
Authors: Fernández-Martín Silvia , González-Cantalapiedra Antonio , Muñoz Fernando , García-González Mario , Permuy María , López-Peña Mónica
Published: May 2021

Glucosamine and chondroitin sulfate have been proposed due to their physiological and functional benefits in the management of osteoarthritis in companion animals. However, the scientific evidence for their use is still controversial. The purpose of this review was to critically elucidate the efficacy of these nutraceutical therapies in delaying the progression of osteoarthritis, evaluating their impact on the synovial knee joint tissues and biochemical markers in preclinical studies by systematically reviewing the last two decades of peer-reviewed publications on experimental osteoarthritis. Three databases (PubMed, Scopus and, Web of Science) were screened for eligible studies. Twenty-two articles were included in the review. Preclinical studies showed a great heterogeneity among the experimental designs and their outcomes. Generally, the evaluated nutraceuticals, alone or in combination, did not seem to prevent the subchondral bone changes, the synovial inflammation or the osteophyte formation. However, further experimental studies may be needed to evaluate their effect at those levels. Regarding the cartilage status and biomarkers, positive responses were identified in approximately half of the evaluated articles. Furthermore, beneficial effects were associated with the pre-emptive administrations, higher doses and, multimodality approaches with some combined therapies. However, additional studies in the long term and with good quality and systematic design are required.
Single-Step Genome Wide Association Study Identifies QTL Signals for Untrimmed and Trimmed Thigh Weight in Italian Crossbred Pigs for Dry-Cured Ham Production
Authors: Palombo Valentino , D’Andrea Mariasilvia , Licastro Danilo , Dal Monego Simeone , Sgorlon Sandy , Sandri Misa , Stefanon Bruno
Published: May 2021

Protected Designation of Origin (PDO) dry-cured ham is the most important product in the Italian pig breeding industry, mainly oriented to produce heavy pig carcasses to obtain hams of the right weight and maturity. Recently, along with the traditional traits swine breeding programs have aimed to include novel carcass traits. The identification at the genome level of quantitative trait loci (QTLs) affecting such new traits helps to reveal their genetic determinism and may provide information to be integrated in prediction models in order to improve prediction accuracy as well as to identify candidate genes underlying such traits. This study aimed to estimate genetic parameters and perform a single step genome wide association studies (ssGWAS) on novel carcass traits such as untrimmed (UTW) and trimmed thigh weight (TTW) in two pig crossbred lines approved for the ham production of the Italian PDO. With this purpose, phenotypes were collected from ~1800 animals and 240 pigs were genotyped with Illumina PorcineSNP60 Beadchip. The single-step genomic BLUP procedure was used for the heritability estimation and to implement the ssGWAS. QTL were characterized based on the variance of 10-SNP sliding window genomic estimated breeding values. Moderate heritabilities were detected and QTL signals were identified on chromosome 1, 4, 6, 7, 11 and 15 for both traits. As expected, the genetic correlation among the two traits was very high (~0.99). The QTL regions encompassed a total of 249 unique candidate genes, some of which were already reported in association with growth, carcass or ham weight traits in pigs. Although independent studies are required to further verify our findings and disentangle the possible effects of specific linkage disequilibrium in our population, our results support the potential use of such new QTL information in future breeding programs to improve the reliability of genomic prediction.
Evaluation of Gilthead Seabream (Sparus aurata) Immune Response after LCDV-Sa DNA Vaccination
Authors: Leiva-Rebollo Rocío , Castro Dolores , Moreno Patricia , Borrego Juan J. , Labella Alejandro M.
Published: May 2021

Lymphocystis disease is the main viral pathology reported in gilthead seabream. Its etiological agent is Lymphocystis disease virus 3 (LCDV-Sa), genus Lymphocystivirus, family Iridoviridae. There are no effective treatments or vaccines for LCDV control, thus the main aim of this study was to develop a DNA vaccine, and to evaluate both the protection conferred against LCDV-Sa infection and the immune response in vaccinated fish. The vaccine was constructed by cloning the mcp gene (ORF LCDVSa062R) into pcDNA3.1/NT-GFP-TOPO. Two independent vaccination trials were conducted. In the first one, 5–7 g fish were intramuscularly injected with the vaccine (pcDNA-MCP) or the empty-plasmid, and the distribution and expression of the vaccine was investigated. Furthermore, vaccinated fish were challenged with LCDV-Sa in order to access the protective capacity of the vaccine. In the second trial, 70–100 g fish were vaccinated as specified, and the immune response was evaluated analyzing the expression of 23 immune-related genes and the production of specific antibodies. The results showed that the vaccine triggers an immune response characterized by the overexpression of genes relating to the inflammatory process, but not the innate antiviral immunity relating to type I IFN (interferon), and also induces the production of specific neutralizing antibodies, which could explain the protection against LCDV-Sa in vaccinated fish.
Feeding System Resizes the Effects of DGAT1 Polymorphism on Milk Traits and Fatty Acids Composition in Modicana Cows
Authors: Tumino Serena , Criscione Andrea , Moltisanti Valentina , Marletta Donata , Bordonaro Salvatore , Avondo Marcella , Valenti Bernardo
Published: May 2021

The interaction between genetic polymorphism and feeding system on milk traits and fatty acid composition was investigated in Modicana cows. Two DGAT1 K232A genotypes (AK and AA) and two feeding regimes, extensive system (EX) with 8 h of grazing without concentrate (EX) and semi-intensive systems (SI) with 2 h of grazing with concentrate, were investigated. DGAT1 genotype did not influence milk yield and composition. The feeding system affected milk composition: protein was significantly higher in SI and lactose in the EX system. A significant genotype × feeding system interaction was observed: the protein and casein levels of AK cows were higher in the SI compared to the EX system. Milk fatty acids profile, total saturated to total unsaturated fatty acids, n-6 to n-3 ratios, and atherogenic index were affected by the feeding system, improving the healthy properties of milk from animals reared in the extensive system. DGAT1 genotype influenced the fatty acid composition: milk from AA cows had a more favorable fatty acid composition due to lower total saturated fatty acids, saturated to unsaturated ratio, atherogenic index, and higher levels of oleic acid and total unsaturated fatty acids. Furthermore, an interaction genotype x feeding system was observed: the AK milk was richer in short-chain FAs (C4:0–C8:0) and C10:0 only in the EX but not in the SI system. Our data suggest that a high amount of green forage in the diet of Modicana cows can resize the effect of the DGAT1 genotype on milk traits and fatty acids composition.
Seasonal Variations of Faecal Cortisol Metabolites in Koalas in South East Queensland
Authors: Santamaria Flavia , Palme Rupert , Schlagloth Rolf , Klobetz-Rassam Edith , Henning Joerg
Published: May 2021

The Koala (Phascolarctos cinereus) is an endemic marsupial inhabiting four states of Australia. Urbanisation, declining habitat, drought and fires are threatening the survival of this flagship species. These threats may cause acute and chronic stress in koalas, which might also be associated with occurrence of infectious diseases in koala populations. Stress may induce an increase in cortisol reflected in increased faecal cortisol metabolite (FCM) values. To be able to use faecal cortisol metabolites to measure stress levels in this species, our aim was to determine baseline values for males and females during breeding and non-breeding season. A total of 351 defecations were collected fortnightly, twice a day, for 12 months from koalas at a wildlife facility in South East Queensland. Samples were analysed with three different enzyme immunoassays (EIAs): a cortisol, 5α-pregnane-3β,11β,21-triol-20-one (37e) and tetrahydrocorticosterone (50c) EIA. The latter, which also reacts with tetrahydrocortisol, the main metabolite in koala faeces, was found to have the highest biological sensitivity and, therefore, is the most suitable EIA to measure stress levels in koalas. Utilising this EIA, we found significant differences (p < 0.05) in FCM values between males and females, breeding and non-breeding season, and between morning and evening samples. Values of faecal cortisol metabolites established in stress-free koalas in this study can serve as a reference for future studies in koalas.
Prevalence and Antibiogram of Vibrio parahaemolyticus and Aeromonas hydrophila in the Flesh of Nile Tilapia, with Special Reference to Their Virulence Genes Detected Using Multiplex PCR Technique
Authors: Zaher Hanan A. , Nofal Mohamad I. , Hendam Basma M. , Elshaer Moustafa M. , Alothaim Abdulaziz S. , Eraqi Mostafa M.
Published: May 2021

Vibrio parahaemolyticus and Aeromonas hydrophila are major public health problems and the main cause of bacterial disease in Nile tilapia (Oreochromis niloticus). This study was conducted to determine the prevalence, antibiotic resistance and some virulence genes of both V. parahaemolyticus and A. hydrophila isolates from Nile tilapia. From Manzala Farm at Dakahlia governorate, 250 freshwater fish samples were collected. The confirmed bacterial isolates from the examined Nile tilapia samples in the study were 24.8% (62/250) for V. parahaemolyticus and 19.2% (48/250) for A. hydrophila. multiplex PCR, revealing that the tlh gene was found in 46.7% (29/62) of V. parahaemolyticus isolates, while the tdh and trh virulence genes were found in 17.2% (5/29). Meanwhile, 39.5% (19/48) of A. hydrophila isolates had the 16s rRNA gene and 10.5% (2/19) had the aerA and ahh1 virulence genes. The Multiple Antibiotic Resistance indices of V. parahaemolyticus and A. hydrophila were 0.587 and 0.586, respectively. In conclusion, alternative non-antibiotic control strategies for bacterial infections in farmed fish should be promoted to avoid multidrug-resistant bacteria. Therefore, it is suggested that farmers should be skilled in basic fish health control and that molecular detection methods are more rapid and cost-effective than bacteriological methods.
Dalbavancin for the Treatment of Prosthetic Joint Infections: A Narrative Review
Authors: Buzón-Martín Luis , Zollner-Schwetz Ines , Tobudic Selma , Cercenado Emilia , Lora-Tamayo Jaime
Published: May 2021

Dalbavancin (DAL) is a lipoglycopeptide with bactericidal activity against a very wide range of Gram-positive microorganisms. It also has unique pharmacokinetic properties, namely a prolonged half-life (around 181 h), which allows a convenient weekly dosing regimen, and good diffusion in bone tissue. These features have led to off-label use of dalbavancin in the setting of bone and joint infection, including prosthetic joint infections (PJI). In this narrative review, we go over the pharmacokinetic and pharmacodynamic characteristics of DAL, along with published in vitro and in vivo experimental models evaluating its activity against biofilm-embedded bacteria. We also examine published experience of osteoarticular infection with special attention to DAL and PJI.
Solution-Processed Memristor Devices Using a Colloidal Quantum Dot-Polymer Composite
Authors: Kim Minho , Oh Seongkeun , Song Seungho , Kim Jiwan , Kim Yong-Hoon
Published: May 2021

In this study, we demonstrate solution-processed memristor devices using a CdSe/ZnS colloidal quantum dot (CQD)/poly(methyl methacrylate) (PMMA) composite and their electrical characteristics were investigated. Particularly, to obtain stable memristive characteristics with a large current switching ratio, the concentration of CdSe/ZnS QDs in the PMMA matrix was optimized. It was found that with the CdSe/ZnS QD concentration of 1 wt%, the memristor device exhibited a high current switching ratio of ~104 and a retention time over 104 s, owing to the efficient charge trapping and de-trapping during the set and reset processes, respectively. In addition, we investigated the operational stability of the device by carrying out the cyclic endurance test and it was found that the memristor device showed stable switching behavior up to 400 cycles. Furthermore, by analyzing the conduction behavior of the memristor device, we have deduced the possible mechanisms for the degradation of the switching characteristics over long switching cycles. Specifically, it was observed that the dominant conduction mechanism changed from trap-free space charge-limited current conduction to trap charge-limited current conduction, indicating the creation of additional trap states during the repeated operation, disturbing the memristive operation.
A Fall Posture Classification and Recognition Method Based on Wavelet Packet Transform and Support Vector Machine
Authors: Zhang Qingyun , Tao Jin , Sun Qinglin , Zeng Xianyi , Dehmer Matthias , Zhou Quan
Published: May 2021

An accidental fall seriously threatens the health and safety of the elderly. The injuries caused by a fall have a lot to do with the different postures during the fall. Therefore, recognizing the posture of falling is essential for the rescue and care of the elderly. In this paper, a novel method was proposed to improve the classification and recognition accuracy of fall postures. Firstly, the wavelet packet transform was used to extract multiple features from sample data. Secondly, random forest was used to evaluate the importance of the extracted features and obtain effective features through screening. Finally, the support vector machine classifier based on the linear kernel function was used to realize the falling posture recognition. The experiment results on “Simulated Falls and Daily Living Activities Data Set” show that the proposed method can distinguish different types of fall postures and achieve 99% classification accuracy.
Environmentally Stable Chiral-Nematic Liquid-Crystal Elastomers with Mechano-Optical Properties
Authors: Ku Kyosun , Hisano Kyohei , Kimura Seiya , Shigeyama Tomoki , Akamatsu Norihisa , Shishido Atsushi , Tsutsumi Osamu
Published: May 2021

Chiral-nematic liquid crystal (N* LC) elastomers exhibit mechano-optical responsive behavior. However, practical sensor applications have been limited by the intrinsic sensitivity of N* LC elastomers to environmental conditions, such as temperature. Although densely cross-linked LC network polymers exhibit high thermal stability, they are not proper for the mechanical sensor due to high glass transition temperatures and low flexibility. To overcome these issues, we focused on enhancing thermal stability by introducing noncovalent cross-linking sites via intermolecular interactions between LC molecules bonded to the polymer network. N* LC elastomers with a cyanobiphenyl derivative as a side-chain mesogen exhibited mechano-optical responsive behavior, with a hypsochromic shift of the reflection peak wavelength under an applied tensile strain and quick shape and color recovery owing to high elasticity. Notably, the N* LC elastomers showed high resistance to harsh environments, including high temperatures and various solvents. Interactions, such as π–π stacking and dipole–dipole interactions, between the cyanobiphenyl units can act as weak cross-links, thus improving the thermal stability of the LC phase without affecting the mechano-optical response. Thus, these N* LC elastomers have great potential for the realization of practical mechano-optical sensors.
Reference Mapping Considering Swaps of Adjacent Bases
Authors: Kim Youngho , Kang Munseong , Jeong Ju-Hui , Kang Dae Woong , Park Soo Jun , Sim Jeong Seop
Published: May 2021

Since the time of the HGP, research into next-generation sequencing, which can reduce the cost and time of sequence analysis using computer algorithms, has been actively conducted. Mapping is a next-generation sequencing method that identifies sequences by aligning short reads with a reference genome for which sequence information is known. Mapping can be applied to tasks such as SNP calling, motif searches, and gene identification. Research on mapping that utilizes BWT and GPU has been undertaken in order to obtain faster mapping. In this paper, we propose a new mapping algorithm with additional consideration for base swaps. The experimental results demonstrate that when the penalty score for swaps was −1, −2, and −3 in paired-end alignment, for the human whole genome, SOAP3-swap aligned 4667, 2318, and 972 more read pairs, respectively, than SOAP3-dp, and for the drosophila genome, SOAP3-swap aligned 1253, 454, and 129 more read pairs, respectively, than SOAP3-dp. SOAP3-swap has the same functionality as that of SOAP3-dp and also improves the alignment ratio by taking biologically significant swaps into account for the first time.
Applications of Smart Helmet in Applied Sciences: A Systematic Review
Authors: Choi Yosoon , Kim Yeanjae
Published: May 2021

A smart helmet is a wearable device that has attracted attention in various fields, especially in applied sciences, where extensive studies have been conducted in the past decade. In this study, the current status and trends of smart helmet research were systematically reviewed. Five research questions were set to investigate the research status of smart helmets according to the year and application field, as well as the trend of smart helmet development in terms of types of sensors, microcontrollers, and wireless communication technology. A total of 103 academic research articles published in the past 11 years (2009–2020) were analyzed to address the research questions. The results showed that the number of smart helmet applications reported in literature has been increasing rapidly since 2018. The applications have focused mostly on ensuring the safety of motorcyclists. A single-board-based modular concept unit, such as the Arduino board, and sensor for monitoring human health have been used the most for developing smart helmets. Approximately 85% of smart helmets have been developed to date using wireless communication technology to transmit data obtained from smart helmets to other smart devices or cloud servers.
Resonance Fatigue Behaviour of Concretes with Recycled Cement and Aggregate
Authors: Cantero Blas , Sainz-Aja Jose , Yoris Adrian , Medina César , Thomas Carlos
Published: May 2021

The huge increase in production of construction and demolition waste (CDW) worldwide is leading to the valorisation of as recycled aggregates. One of the most promising alternatives is its use as a recycled aggregate in the manufacture of structural concrete, which motivates the study of the dynamic behaviour of these materials in order to ensure their suitability for use in elements subjected to dynamic loads. This work evaluated the resonant compressive fatigue behaviour of structural concretes with 25% or 50% recycled mixed aggregates, either individually or in combination with 25% recycled cement of clay-based materials both from CDW. All mixes were subjected to compressive fatigue tests using the accelerated Locati method. Regarding the fatigue limit, the results showed that for all mixes, it was between 30% and 45% of the compressive strength. In addition, a correlation was also found between the resonance frequency of the test and the deformation suffered by the specimen. This correlation enabled the estimation of the fatigue limit through a more stable parameter than the strain measured by strain gauges, namely, the resonance frequency. In addition, it was found that the resonance frequency of the test changed as the specimen damage increased. This observation enabled the estimation of the fatigue limit through a more stable parameter than the strain measured by strain gauges, namely, the resonance frequency.
HVdc Circuit Breakers: Prospects and Challenges
Authors: Raza Ali , Mustafa Ali , Alqasemi Umar , Rouzbehi Kumars , Muzzammel Raheel , Guobing Song , Abbas Ghulam
Published: May 2021

The integration of offshore wind farms has revitalized the interest in multi–terminal high voltage direct current (M–HVdc) transmission grids. HVdc breakers’ importance has increased as M–HVdc grids are now a commercial truth. Several HVdc circuit breaker technologies have been developed, published, and appeared as prototypes for HVdc networks. This paper summarizes the HVdc breaker technologies from the last two decades, distributed mainly in literature. A comparison of various state–of–the–art HVdc breakers is presented. Further, areas are identified where further research and development are required. The goal is to provide primary challenges and prospects in the HVdc breaker field.