bluuebunny/crossref_metadata_2025 huggingface dataset:
Performance evaluation of generalized selection diversity systems over Nakagami‐m fading channels
Authors: Annamalai A. , Tellambura C.
Published: February 2003

The generalized selection combining (GSC) scheme that adaptively combines a subset of M strongest paths out of L available diversity paths finds applications in several wideband receivers and broadband wireless communications. In this paper, exact closed‐form expressions for the moment generating function (MGF), the probability density function (PDF) and the cumulative density function (CDF) of the GSC(M, L) output signal‐to‐noise ratio (SNR) in independent and identically distributed (i.i.d) Nakagami‐m fading channels are derived while the fading index is a positive integer. These expressions hold for any M and L and provide a comprehensive framework for performance analysis including the derivation of closed‐form formulas for the average symbol error probability (ASEP) of a broad class of binary and M‐ary modulations, mean combined SNR and the outage probability of GSC(M, L) receiver structures. When the Nakagami‐m fading index is not an integer, the MGF of GSC(M, L) output SNR is derived as an (M − 1)‐fold infinite series. With this MGF, analytical expressions for both the outage probability and error rates can be readily obtained. An easily programmable recursive solution of the MGF of GSC(M, L) output SNR is also outlined for both the positive integer and noninteger fading severity index cases. Copyright © 2002 John Wiley & Sons, Ltd.
Performance evaluation of an optimized intersegment handover procedure for hybrid constellation satellite systems
Authors: Leo M. , Luglio M.
Published: February 2003

Satellite system architectures based on geostationary or Low Earth Orbit (LEO)/Medium Earth Orbit (MEO) constellation suffer from their intrinsic limitations in terms of coverage or flexibility. Traffic requirements concerning broadband services are expected to be very uneven both in time and space. To match this requirement and to improve coverage an innovative satellite system architecture, composed of a LEO/MEO segment to complement a Geostationary Earth Orbit (GEO) segment, has been proposed. In this scenario, to achieve interworking and to make it possible to hold the call while switching between the two segments, efficient intersegment handover (ISHO) procedures must be identified.

The paper, after introducing the classical ISHO schemes, aims at defining and analysing an ISHO procedure developed to perform handover in case of hybrid constellations based on the use of both GEO and LEO/MEO orbits. Performance evaluation will be carried out for different system configurations utilizing a dynamic satellite constellation simulator in the time domain. The execution delay and its complementary cumulative distribution have been evaluated for different constellation geometry at different distances from the gateway. Copyright © 2002 John Wiley & Sons, Ltd.
Uplink channel assignment with balanced load for code division multiple access cellular systems
Authors: Chang Ruay‐Shiung , Hsieh Chih‐Tsung
Published: February 2003

In a code division multiple access (CDMA) wireless communication system, each mobile handset must be power controlled such that the power received at the base station is roughly the same. Otherwise, the interferences between mobile handsets will degrade the performance and increase the error rate. When a mobile handset uses channels from the neighbouring cells, it will raise its power to meet the threshold of signal strength. This will also increase the interference in the home cell. Therefore, we do not want a mobile handset to use channels from other cells blindly. In this paper, we propose an uplink channel assignment method based on the directed retry concept for CDMA cellular systems. The purpose is to achieve load balancing between neighbouring cells and at the same time controlling the interference levels at the base stations such that it will not affect the performance. Furthermore, priorities are given to handoff calls when assigning channels. Copyright © 2002 John Wiley & Sons, Ltd.
QoS‐based adaptive error control for wireless networks
Authors: Nikaein Neda , Bonnet Christian
Published: February 2003

Wireless channels are highly affected by unpredictable factors such as cochannel interference, adjacent channel interference, propagation path loss, shadowing, and multipath fading. The unreliability of media seriously degrades the transmission quality. Automatic Repeat reQuest (ARQ) and Forward Error Correction (FEC) schemes are frequently used in wireless environments to reduce the high bit error rate of the channel. In this paper, we propose an adaptive error‐control scheme for wireless networks on the basis of dynamic variation of error‐control strategy as a function of the channel bit error rate, desired QoS, and number of receivers. Reed–Solomon codes are used throughout this study because of their appropriate characteristics in terms of powerful coding and implementation simplicity. Simulation results show that our adaptive error‐control protocol decreases the waste of bandwidth due to retransmissions or extra coding overheads while satisfying the QoS requirements of the receivers. Copyright © 2002 John Wiley & Sons, Ltd.
Effect of fading, diversity and interference on the performance of multitone CDMA systems
Authors: Matin Sharif A. , Ugweje Okechukwu C.
Published: February 2003

The performance of a multitone Code Division Multiple Access (CDMA) system is investigated in this paper by considering the effect of multipath fading, diversity and narrowband interference. The performance is evaluated in terms of the average bit error rate when both multiple access and partial band interference are present. Results are obtained by deriving the statistics of the receiver output parameters and then tested by numerical evaluation. The bit error rate performance is analyzed as a function of bit rate, bandwidth, delay range and different diversity techniques, while considering issues relevant to system implementation. Comparisons were made between conventional single‐tone CDMA systems and multitone CDMA systems. The effectiveness of multitone CDMA in suppressing interference compared to conventional CDMA system is analyzed. Results reveal considerable performance improvement of multitone CDMA system over conventional CDMA system. Copyright © 2002 John Wiley & Sons, Ltd.
Providing deterministic packet delays and packet losses in multimedia wireless networks
Authors: Hamdi Mounir , Lee Felix K. L.
Published: February 2003

‘Anytime, anywhere’ communication, information access and processing are much cherished in modern societies because of their ability to bring flexibility, freedom and increased efficiency to individuals and organizations. Wireless communications, by providing ubiquitous and tetherless network connectivity to mobile users, are therefore bound to play a major role in the advancement of our society. Although initial proposals and implementations of wireless communications are generally focused on near‐term voice and electronic messaging applications, it is recognized that future wireless communications will have to evolve towards supporting a wider range of applications, including voice, video, data, images and connections to wired networks. This implies that future wireless networks must provide quality‐of‐service (QoS) guarantees to various multimedia applications in a wireless environment.

Typical traffic in multimedia applications can be classified as either Constant‐Bit‐Rate (CBR) traffic or Variable‐Bit‐Rate (VBR) traffic. In particular, scheduling the transmission of VBR multimedia traffic streams in a wireless environment is very challenging and is still an open problem. In general, there are two ways to guarantee the QoS of VBR multimedia streams, either deterministically or statistically. In particular, most connection admission control (CAC) algorithms and medium access control (MAC) protocols that have been proposed for multimedia wireless networks only provide statistical, or soft, QoS guarantees.

In this paper, we consider deterministic QoS guarantees in multimedia wireless networks. We propose a method for constructing a packet‐dropping mechanism that is based on a mathematical framework that determines how many packets can be dropped while the required QoS can still be preserved. This is achieved by employing: (1) An accurate traffic characterization of the VBR multimedia traffic streams; (2) A traffic regulator that can provide bounded packet loss and (3) A traffic scheduler that can provide bounded packet delay. The combination of traffic characterization, regulation and scheduling can provide bounded loss and delay deterministically. This is a distinction from traditional deterministic QoS schemes in which a 0% packet loss are always assumed with deterministically bounding the delay.

We performed a set of performance evaluation experiments. The results will demonstrate that our proposed QoS guarantee schemes can significantly support more connections than a system, which does not allow any loss, at the same required QoS. Moreover, from our evaluation experiments, we found that the proposed algorithms are able to out‐perform scheduling algorithms adopted in state‐of‐the‐art wireless MAC protocols, for example Mobile Access Scheme Based on Contention and Reservation for ATM (MASCARA) when the worst‐case traffic is being considered. Copyright © 2002 John Wiley & Sons, Ltd.
Adaptive delivery of video data over wireless and mobile environments
Authors: Helal Sumi , Sampath Latha , Birkett Kevin , Hammer Joachim
Published: February 2003

We present an architecture for the adaptable delivery of video data under variable connection characteristics and into devices of variable capabilities. The main application of the proposed architecture is video delivery in wireless and mobile environments. The architecture is based on the Universal Multimedia Access concept and the MPEG‐7 standard. Based on the network and the mobile device, as well as constraints imposed by user preferences and the multimedia content, video is delivered through a careful application of a combination of off‐line and on‐line reductions to the video stream. We present our architecture and describe an implementation of a system based on the architecture. We present basic performance evaluation results to quantify the merit of our approach. Copyright © 2002 John Wiley & Sons, Ltd.
Effects of Artificial Ultraviolet Light Exposure on Reproductive Success of the Female Panther Chameleon (Furcifer pardalis) in Captivity
Authors: Ferguson Gary W. , Gehrmann W.H. , Chen T.C. , Dierenfeld E.S. , Holick M.F.
Published: February 2003

Having previously documented experimentally the need for ultraviolet B (UVB) irradiation (290–315 nm) in the light environment of captive female panther chameleons (Furcifer pardalis) to ensure hatching success of their eggs, we investigated optimal UVB irradiation levels. From 1996–1998 28 hatchling female panther chameleons were raised to maturity and bred (using vitamin and mineral‐fortified insect diets low in vitamin D) in nine different artificial UVB light environments. Seven of the environments included long (12 hr/day) low irradia‐tion exposures ranging from 1.7 to 22 μW/cm2 UVB, with a corresponding conversion of provitamin D3 to photoproducts in in vitro models of 0.18 to 1.52% in 2 hr. Two environments included short (0.5 and 1.0 hr/day), high irradiation exposures of 55 and 49 μW/cm2 UVB, respectively, with a corres‐ponding conversion of provitamin D3 to photoproducts in in vitro models of 8.3% to 14.6% in the respective 0.5‐ and 1.0‐hr time periods. Females raised with the mid‐level long/low exposures (5–15 μW/cm2 UVB; 0.52–1.32% conversion to photoproducts in in vitro models) produced viable eggs with a significantly higher percentage of hatching compared to those with the extreme (highest or lowest) long/low exposures. Those raised with the short‐/high‐exposure environments produced viable eggs with a generally high percentage of hatching, but success was variable. The results and techniques for light quality assessment are interpreted, with recommendations for practical application by the herpetoculturist desiring to successfully breed panther chameleons in captivity. Zoo Biol 21:525–537, 2002. © 2002 Wiley‐Liss, Inc.
Genetic kinship and social structure in a herd of square‐lipped rhinoceroses (Ceratotherium simum simum) at the zoological center, Tel Aviv/Ramat‐Gan, Israel
Authors: Seror Benjamin , Zorovsky Yehuda , Terkel Amelia , Katcoff Don J.
Published: February 2003

Zoos and zoological parks serve as genetic and demographic reserves for strengthening endangered populations and reestablishing extinct populations in the wild. Knowing the genetic ties within captive populations is a very helpful tool for successful reproductive management. In the present study we addressed kinship relationships and behavior among rhinoceroses (Ceratotherium simum simum) raised at the Zoological Center, Tel Aviv/Ramat Gan, Israel, with the hope of identifying reasons for the declining rate of reproduction within the herd. We used the random amplified polymorphic DNA (RAPD) technique to reveal the paternity of the rhinos born at the park. In this way, we identified the paternity of five out of seven young born in the herd, which are currently in Ramat Gan. One male accounted for three (37.5%) births, and two other males accounted for one each. The paternity of the two other animals is unknown and may be of animals that are no longer in the Zoological Center. The genetic determinations were accompanied by behavioral observations, which enabled us to determine the social dynamics in the herd. This study suggests that there are at least three contributing factors to the reproductive decline in the herd: 1) a surplus of males, 2) exclusion of potentially reproductive males from the breeding stock, and 3) specific behavioral and physiological problems in some members of the herd. Zoo Biol 21:561–571, 2002. © 2002 Wiley‐Liss, Inc.
Responses to novel foods in captive chimpanzees
Authors: Visalberghi E. , Myowa Yamakoshi M. , Hirata S. , Matsuzawa T.
Published: February 2003

Hesitancy to eat novel foods hampers the immediate enlargement of the diet but serves to limit the risk of ingesting toxic foods. Neophobia has been systematically investigated in only a few primate species, in which it appears to be affected by social influences. Surprisingly, little is known about neophobia in chimpanzees. We studied the response of eight adult captive chimpanzees to 16 foods (foods commonly eaten by humans and never tasted before by chimpanzees). Each novel food was presented twice to the chimpanzee by a familiar or an unfamiliar human. Between the two trials the human ate the food face to face with the chimpanzee (demonstration). Results showed that some foods were almost unanimously accepted, whereas others were not. Moreover, there were marked interindividual differences in food acceptance and consumption; chimpanzees ranged from being almost completely neophobic to accepting almost all foods. Familiarity with the human and the human's demonstration did not affect responses to the foods. The humans' predictions concerning the chimpanzees' acceptance of the different foods were rather good; furthermore, in seven cases out of eight the humans' preferences did not correlate with their predictions on the chimpanzees' preferences. The finding that most captive chimpanzees are initially cautious toward novel foods supports the little information there is regarding this subject in wild chimpanzees. However, the lack of influence of the humans' familiarity and demonstration on the response to food by the chimpanzees calls for more naturalistic studies, in which social influences are provided by group members. Since novel stimuli provide sensory stimulation and elicit exploration and social interest, occasional presentation of novel foods could be a promising and cheap device for feeding enrichment. Zoo Biol 21:539–548, 2002. © 2002 Wiley‐Liss, Inc.
Change of enclosure in langur monkeys: implications for the evaluation of environmental enrichment
Authors: Little Katherine A. , Sommer Volker
Published: February 2003

A group of Hanuman langurs (Presbytis entellus) was studied before and after it was moved from an old cage‐style enclosure to a novel naturalistic environment at the London Zoo. Eating and locomotion occupied more of the langurs' time in their new enclosure, whereas dozing, allogrooming, and aggression decreased, along with an increase in inter‐individual distances. These changes are attributed to the larger area, the stimulating new environment, and the langurs' increased distance from visitors. Nevertheless, the study raises questions about how to define standards of desirable environmental enrichment, as the activity patterns recorded in both the old and new enclosures are within the variation observed in the wild. Zoo Biol 21:549–559, 2002. © 2002 Wiley‐Liss, Inc.
Effect of feeding boxes on the behavior of stereotyping amur tigers (Panthera tigris altaica) in the Zurich Zoo, Zurich, Switzerland
Authors: Jenny Saskia , Schmid Hans
Published: February 2003

The stereotyped pacing shown by the two Amur tigers in the Zurich Zoo was hypothesized as being caused by permanently frustrated appetitive foraging behavior. Several electrically controlled feeding boxes were installed and access to each box was possible only twice a day for 15 min at semi‐random times. The boxes had to be opened actively by the tigers. Two trials were carried out: one with solitary confinement, and one with paired confinement. During box feeding, the female's stereotyped pacing was significantly reduced from 16% (solitary confinement, conventional feeding) and 7% (paired confinement, conventional feeding) to 1% (solitary confinement) and less than 0.01% (paired confinement) of the daily observed time. The female's sleeping increased significantly in both solitary and paired confinement. The male only showed a significant reduction in stereotyped pacing behavior when kept with the female (conventional feeding: 10%; box feeding: <0.01% of the daily observed time). On days with a box‐feeding regime in paired confinement, the male spent 25% (83 min) of the observed time with active behavior at the feeding boxes. The results support the hypothesis that permanently frustrated appetitive foraging behavior causes stereotyped pacing in adult tigers. Zoo Biol 21:573–584, 2002. © 2002 Wiley‐Liss, Inc.
Food access in captive Ammotragus: the role played by hierarchy and mother–infant interactions
Authors: Cassinello Jorge
Published: February 2003

An analysis of individuals' behavior when accessing a restricted food source (troughs) was carried out in a captive population of aoudad (Ammotragus lervia). Access to the troughs followed a strict hierarchical order, as higher‐ranking individuals fed before lower‐ranking ones. Unweaned male and female calves made use of the troughs from the ages of 2 and 3 months, respectively. Both fed from the troughs more frequently and for longer periods when their mother was present, which allowed them to make use of the troughs while skipping the hierarchical order. Calves received fewer threats when in proximity to their mothers, particularly in high‐ranking families. Mothers defended their calves from other herdmates more frequently when at the feeding area than in other areas of the herd. However, only sated mothers let their calves feed freely from the troughs; unsated mothers showed an aggressive behavior even toward their calves. I conclude that a maternal presence is necessary for aoudad calves to successfully feed from troughs, and that families of higher social rank benefit by getting access to this food source earlier in the day and are disturbed less than low‐ranking families. Zoo Biol 21:597–605, 2002. © 2002 Wiley‐Liss, Inc.
Environmental influences on stereotypy and the activity budget of Indian leopards (Panthera pardus) in four zoos in Southern India
Authors: Mallapur Avanti , Chellam Ravi
Published: February 2003

To eliminate abnormal behaviors in leopards (Panthera pardus), such as stereotypic pacing, by utilizing environmental enrichment techniques, a proper understanding of their behavior in captive environments is required. Hence there is a need for animal welfare studies in Indian zoos. The activity budgets of 16 leopards were recorded across four southern Indian zoos: Thiruvananthapuram Zoo, Arignar Anna Zoological Park, Shri Chamarajendra Zoological Gardens, and the Guindy Children's Park. Of the 16 study animals, 14 were studied on‐exhibit on zoo holidays as well as on days with visitors present, and all 16 individuals were studied off‐exhibit on other days with visitors present. The 11 behaviors recorded were categorized into active, resting, and stereotypic behaviors. Leopards exhibited higher levels of activity in the on‐exhibit enclosures on days with no visitors. Feeding time influenced the behavioral repertoire of all 14 leopards studied on‐exhibit. Lower proportions of resting were exhibited during the hours before feeding. The proportion of active behaviors differed significantly across zoos. Stereotypic pacing levels were not influenced by the presence of visitors or by feeding time, but was significantly influenced by enclosure features. Higher levels of stereotypic pacing were exhibited in off‐exhibit than on‐exhibit enclosures. Our study shows that the behavior of captive leopards is influenced by enclosure type, feeding regime, and the presence of visitors. Zoo Biol 21:585–595, 2002. © 2002 Wiley‐Liss, Inc.
Ladder Use and Clubbing by a Bonobo (Pan paniscus) in Apenheul Primate Park
Authors: Gold Kenneth C.
Published: February 2003

Two types of tool use were shown by a captive bonobo (Pan paniscus) in a large outdoor enclosure at Apenheul Primate Park in The Netherlands. A wild‐born young adult female (estimated to be 9 years old) used fallen branches as ladders to bypass protective sheaths to gain access to trees. Later she used a branch as a weapon to beat to death a peahen (Pavo cristatus). None of the other bonobos in the group were seen to use branches as either ladders or weapons. Zoo Biol 21:607–611, 2002. © 2002 Wiley‐Liss, Inc.
First report of albinism in the white‐spotted bamboo shark, Chiloscyllium plagiosum (Orectolobiformes: Hemiscyllidae), with a review of reported color aberrations in elasmobranchs
Authors: Clark Steven
Published: February 2003

Three albinistic, white‐spotted bamboo sharks (Chiloscyllium plagiosum) (two males and one female) were hatched at SeaWorld parks. Due to their lack of integumentary and retinal pigments, all three animals were considered true albinos. The term “leucism,” more prominently used within the herpetological discipline, is suggested as a more apt description for previously reported color aberrations in elasmobranchs. Reports of color aberrations in other species of elasmobranchs, and a clarification of the terms used to describe these irregularities in pigmentation, are also presented. Zoo Biol 21:519–524, 2002. © 2002 Wiley‐Liss, Inc.
Characterization and isolation of novel microsatellites from the Drosophila dunni subgroup
Authors: WILDER J. A. , DIAZ T. , O'NEILL R. J. W. , KENNEY J. , HOLLOCHER H.
Published: February 2003

We have isolated and characterized 77 novel microsatellites from two species, Drosophila dunni and Drosophila nigrodunni, which are closely related Caribbean-island endemics from the Drosophila cardini species group. These species are very distantly related to all other Drosophila from which microsatellites have previously been characterized. We find that the average length of microsatellites isolated in these species is quite small, with an overall mean length of 9·8 repeat units for dinucleotide microsatellites in the two study species. The nucleotide composition of dinucleotides differs between the two species: D. nigrodunni has a predominance of (AC/GT)n repeats, whereas D. dunni has equal numbers of (AC/GT)n and (AG/CT)n repeats. Tri- and tetranucleotide repeats are not abundant in either species. We assayed the variability of eight microsatellites in a closely related third species, Drosophila arawakana, using wild-caught individuals from the island of Guadeloupe. We found the microsatellites to be extremely variable in this population, with observed heterozygosities ranging from 0·541 to 0·889. DNA amplification trials suggest that these eight microsatellites are widely conserved across the D. cardini group, with five of the eight producing amplification products in every species tested. However, the loci are very poorly conserved over greater phylogenetic distances. DNA amplification of the microsatellite loci was unreliable in members of the closely related Drosophila quinaria, Drosophila calloptera, Drosophila guarani and Drosophila tripunctata species groups. Furthermore, these microsatellites could not be detected in the genome of Drosophila melanogaster, despite the conservation of microsatellite flanking regions at some loci. These data indicate that Drosophila microsatellite loci are quite short lived over evolutionary timescales relative to many other taxa.
Variation in Drosophila sensory bristle number at ‘Evolution Canyon’
Authors: LYMAN RICHARD F. , NEVO EVIATAR , MACKAY TRUDY F. C.
Published: February 2003

‘Evolution Canyon’ on Mount Carmel, Israel, displays highly contrasting physical and biotic environments on a micro-geographic scale, and is a natural laboratory for investigating genetic responses to variable and extreme environments across species. Samples of Drosophila melanogaster and D. simulans were collected from three sites each on the north- and south-facing slopes of the canyon along altitudinal transects, and one site on the valley floor. Numbers of abdominal and sternopleural sensory bristles were recorded for each of these subpopulations in three thermal environments. In D. simulans, sternopleural bristle number exhibited micro-geographic differentiation between the north- and south-facing slopes, while abdominal bristle number was stable across subpopulations. In D. melanogaster, the magnitudes of the difference in mean sternopleural bristle number between the north- and south-facing slopes and of mean abdominal bristle number along the altitudinal gradients were both conditional on rearing temperature. Thus, the pattern of genetic variation between sites was consistent with underlying heterogeneity of genetic mechanisms for response to the same environmental gradients between traits and sibling species. In contrast, the genetic architecture of bristle number at the level of variation within populations was very similar between species for the same bristle trait, although the two traits differed in the relative contribution of genotype by temperature and genotype by sex interaction.
Unusual genetic architecture of natural variation affecting drug resistance in Drosophila melanogaster
Authors: CARRILLO ROLAND , GIBSON GREG
Published: February 2003

Naturally occurring genetic variation was quantified for survival time of adult Drosophila melanogaster exposed to chronic ingestion of the drugs nicotine, caffeine, dopamine, tyramine and octopamine. Responses to nicotine, tyramine and octopamine were genetically correlated in both sexes, whereas caffeine response correlated with starvation resistance. However, there is also genetic variation that is specific for each of the drugs. Females tended to be more resistant than males to nicotine and caffeine but sex-by-genotype interactions were also seen for these drugs and for the response to dopamine. An unusual and complex genetic architecture was observed in crosses between lines with different responses to caffeine ingestion. Additive and dominance components were clearly seen from the analysis of F1 individuals, but increased female resistance to caffeine in backcross generations and increased male sensitivity in F2 generations confused the interpretation of possible epistatic contributions.
On flexible finite polygenic models for multiple-trait evaluation
Authors: BINK MARCO C. A. M.
Published: February 2003

Finite polygenic models (FPM) might be an alternative to the infinitesimal model (TIM) for the genetic evaluation of pedigreed multiple-generation populations for multiple quantitative traits. I present a general flexible Bayesian method that includes the number of genes in the FPM as an additional random variable. Markov-chain Monte Carlo techniques such as Gibbs sampling and the reversible jump sampler are used for implementation. Sampling of genotypes of all genes in the FPM is done via the use of segregation indicators. A broad range of FPM models, some combined with TIM, are empirically tested for the estimation of variance components and the number of genes in the FPM. Four simulation scenarios were studied, including genetic models with 5 or 50 additive independent diallelic genes affecting the traits, and random selection or selection on one of the traits was performed. The results in this study were based on ten replicates per simulation scenario. In the case of random selection, uniform priors on additive gene effects led to posterior mean estimates of genetic variance that were positively correlated with the number of genes fitted in the FPM. In the case of trait selection, assuming normal priors on gene effects also led to genetic variance estimates for the selected trait that were negatively correlated with the number of genes in the FPM. This negative correlation was not observed for the unselected trait. Treating the number of genes in the FPM as random revealed a positive correlation between prior and posterior mean estimates of this number, but the prior hardly affected the posterior estimates of genetic variance. Posterior inferences about the number of genes should be considered to be indicative where trait selection seems to improve the power of distinguishing between TIM and FPM. Based on the results of this study, I suggest not replacing TIM by the FPM, but combining TIM and FPM with the number of genes treated as random, to facilitate a highly flexible and thereby robust method for variance component estimation in pedigreed populations. Further study is required to explore the full potential of these models under different genetic model assumptions.
Simple deterministic identity-by-descent coefficients and estimation of QTL allelic effects in full and half sibs
Authors: NAGAMINE Y. , KNOTT S. A. , VISSCHER P. M. , HALEY C. S.
Published: February 2003

Accurate and rapid methods for the detection of quantitative trait loci (QTLs) and evaluation of consequent allelic effects are required to implement marker-assisted selection in outbred populations. In this study, we present a simple deterministic method for estimating identity-by-descent (IBD) coefficients in full- and half-sib families that can be used for the detection of QTLs via a variance-component approach. In a simulated dataset, IBD coefficients among sibs estimated by the simple deterministic and Markov chain Monte Carlo (MCMC) methods with three or four alleles at each marker locus exhibited a correlation of greater than 0·99. This high correlation was also found in QTL analyses of data from an outbred pig population. Variance component analysis used both the simple deterministic and MCMC methods to estimate IBD coefficients. Both procedures detected a QTL at the same position and gave similar test statistics and heritabilities. The MCMC method, however, required much longer computation than the simple method. The conversion of estimated QTL genotypic effects into allelic effects for use in marker-assisted selection is also demonstrated.
Partial male sterility and the evolution of nuclear gynodioecy in plants
Authors: SCHULTZ STEWART T.
Published: February 2003

Gynodioecy, a genetic dimorphism of females and hermaphrodites, is pertinent to an understanding of the evolution of plant gender, mating and genetic variability. Classical models of nuclear gynodioecy attribute the maintenance of the dimorphism to frequency-dependent selection in which the female phenotype has a fitness advantage at low frequency owing to a doubled ovule fertility. Here, I analyse explicit genetic models of nuclear gynodioecy that expand on previous work by allowing partial male sterility in combination with either fixed or dynamically evolving mutational inbreeding depression. These models demonstrate that partial male sterility causes fitness underdominance at the mating locus, which can prevent the spread of females. However, if partial male sterility is compensated by a change in selfing rate, overdominance at the mating locus can cause the spread of females. Overdominance at introduction of the male sterility allele can be caused by high inbreeding depression and a lower selfing rate in the heterozygote, by purging of mutations by a higher selfing rate in the heterozygote, and by low inbreeding depression and a higher selfing rate in the heterozygote. These processes might be of general importance in the maintenance of mating polymorphisms in plants.
Quantitative trait loci for lifespan of mated Drosophila melanogaster affect both sexes
Authors: REIWITCH SARAH G. , NUZHDIN SERGEY V.
Published: February 2003

The properties of alleles at quantitative trait loci (QTLs) contributing to variation in lifespan should be described to determine the mechanisms of evolution of life length and to predict its future changes. Previously, we and others conducted genome-wide screens for QTLs that segregate among one panel of recombinant inbred lines (RILs) using a dense molecular marker map. In non-stressful conditions, QTLs effecting the lifespans of virgin females and males were frequently sex specific. In an unrelated panel of RILs, the effects of QTLs in flies maintained in cages with mixed sexes were similar in both sexes. Here, we re-measured the lifespans of the former panel of RILs in cages with mixed sex cohorts. Lifespan declined owing to mating. The amount of decline correlated between sexes within lines. QTLs mapping to the intervals 15A–19C, 50B–57C, 63A–65A, and 96F–99B had similar effects on the lifespans of both males and females. These QTLs have previously been detected in virgin flies surveys and had sex- and/or environment-specific effects.
Molecular population genetics of sequence length diversity in the Adh region of Drosophila pseudoobscura
Authors: SCHAEFFER STEPHEN W.
Published: February 2003

Positive and negative selection on indel variation may explain the correlation between intron length and recombination levels in natural populations of Drosophila. A nucleotide sequence analysis of the 3·5 kilobase sequence of the alcohol dehydrogenase (Adh) region from 139 Drosophila pseudoobscura strains and one D. miranda strain was used to determine whether positive or negative selection acts on indel variation in a gene that experiences high levels of recombination. A total of 30 deletion and 36 insertion polymorphisms were segregating within D. pseudoobscura populations and no indels were fixed between D. pseudoobscura and its two sibling species D. miranda and D. persimilis. The ratio of Tajima's D to its theoretical minimum value (Dmin) was proposed as a metric to assess the heterogeneity in D among D. pseudoobscura loci when the number of segregating sites differs among loci. The magnitude of the D/Dmin ratio was found to increase as the rate of population expansion increases, allowing one to assess which loci have an excess of rare variants due to population expansion versus purifying selection. D. pseudoobscura populations appear to have had modest increases in size accounting for some of the observed excess of rare variants. The D/Dmin ratio rejected a neutral model for deletion polymorphisms. Linkage disequilibrium among pairs of indels was greater than between pairs of segregating nucleotides. These results suggest that purifying selection removes deletion variation from intron sequences, but not insertion polymorphisms. Genome rearrangement and size-dependent intron evolution are proposed as mechanisms that limit runaway intron expansion.
Intracellular battlegrounds: conflict and cooperation between transposable elements
Authors: LEONARDO TERESA E. , NUZHDIN SERGEY V.
Published: February 2003

Transposable elements (TEs) are genomic parasites that amplify their own representation on hosts' chromosomes by inserting into new positions. It is traditionally thought that their copy number is regulated by purifying selection that eliminates hosts with higher than average TE abundance. Here, we stress that selection due to beneficial or harmful interactions between TEs introduces a whole new dimension, with implications for TE evolutionary trajectories and TE loads on hosts. This framework poses new questions requiring conceptual and experimental advances. Considering primarily Drosophila data, we make a case for within host selection on TEs by thinking expansively about the lifecycle of several TE families.
Heat-induced interactions of β-lactoglobulin A and κ-casein B in a model system
Authors: Cho Younghee , Singh Harjinder , Creamer Lawrence K
Published: February 2003

The interaction of κ-casein and β-lactoglobulin is fundamental to all heat-induced modifications of milk product functionality, such as the heat stability of concentrated milks. Purified native κ-casein B and β-lg A solutions were heated at 80 °C at pH 6·7 separately and in a mixture. The circular dichroism spectra in the near UV indicated irreversible changes in the disulphide bonding patterns involving both proteins. Alkaline- and SDS-PAGE of heated samples showed that, in the presence of κ-casein, less β-lg was converted into β-lg polymers and the rate of loss of native β-lg was greater. When κ-casein was added to previously heated β-lg and the mixture was heated, the κ-casein reacted with the heat-induced β-lg polymers more readily than with the β-lg native monomers. The formation of β-lg dimers, trimers etc. was diminished. It was concluded that, when β-lg and κ-casein were heated together, β-lg formed thiol-exposed monomers, which reacted with each other or with the native κ-casein depending on the relative concentrations of β-lg and κ-casein. The products of these reactions included some disulphide-bonded 1[ratio ]1 β-lg[ratio ]κ-casein complexes, some monomer κ-casein and a range of large aggregates held together by either or both disulphide bonds and hydrophobic association.
Reduction of immunoreactivity of bovine β-lactoglobulin upon combined physical and proteolytic treatment
Authors: Bonomi Francesco , Fiocchi Alessandro , Frøkiær Hanne , Gaiaschi Antonella , Iametti Stefania , Poiesi Claudio , Rasmussen Patrizia , Restani Patrizia , Rovere Pierpaolo
Published: February 2003

Bovine β-lactoglobulin was hydrolyzed with trypsin or chymotrypsin before, during and after treatment at 600 MPa and pH 6·8 for 10 min at 30, 37 and 44 °C. The extent of β-lactoglobulin hydrolysis under pressure was noticeably higher than at atmospheric pressure, particularly when chymotrypsin was used. Addition of proteases at ambient pressure to previously pressure-treated β-lactoglobulin gave only a modest increase in proteolysis with respect to the untreated protein. Products of enzyme hydrolysis under pressure were separated by reverse-phase HPLC, and were found to be different from those obtained at atmospheric pressure when chymotrypsin was used. The residual immunochemical reactivity of the products of combined pressure-enzyme treatment was assessed on the unresolved hydrolysates by ELISA tests using polyclonal and monoclonal antibodies, and on individual hydrolytic fractions by Western Blotting using sera of paediatric patients allergic to whey proteins in cow milk. The immunoreactivity of the whole hydrolysates was related to their content of residual intact β-lactoglobulin, and no immunochemical reactivity was found for all the products of chymotrypsin hydrolysis under pressure. The results indicate that chymotrypsin effectively hydrolysed hydrophobic regions of β-lactoglobulin that were transiently exposed during the pressure treatments and that were not accessible in the native protein or in the protein that had been previously pressure treated.
Effects of dietary supplements of zinc-methionine on milk production, udder health and zinc metabolism in dairy goats
Authors: Salama Ahmed AK , Caja Gerardo , Albanell Elena , Such Xavier , Casals Ramón , Plaixats Josefina
Published: February 2003

Twenty-two Murciano-Granadina dairy goats were used to investigate the effects of organic Zn supplementation of a diet containing a high level of inorganic Zn. Goats were kept in pens, machine milked once a day throughout lactation and fed a diet based on a dehydrated mixture of whole-plant maize and alfalfa ad libitum, alfalfa pellets, barley grain and a concentrate mixture. Treatments were: (1) control, and (2) supplemented with 1 g/d Zn-Methionine (Zn-Met) included in the concentrate mixture. After parturition, goats were blocked in week 3 and dietary treatments were applied until week 23. From weeks 3–20, feed intake, milk yield, milk composition, milk somatic cell count (SCC), and udder health were measured. In week 21, all goats were injected intraperitoneally with 1 g/d DL-methionine for 5 d to establish the effects of methionine under the conditions of udder stress induced by hand milking on the second day. During weeks 22 and 23, diet digestibility, and N and Zn balance were determined. Dry matter intake, milk yield, and milk contents of total solids, fat, total and true protein, and casein did not differ between treatments, but whey protein and non-protein nitrogen contents were significantly lower for the Zn-Met group. Milk SCC tended to decrease as a result of Zn-Met supplementation but differences between treatments were not significant when halves with persistent infection were excluded. Hand milking increased SCC in both groups, but udders of supplemented goats showed a lower reaction. Apparent absorption of N significantly increased and Zn retention tended to increase in Zn-Met supplemented goats. We conclude that Zn-Met supplementation can enhance resistance to udder stress in dairy goats. Effects were attributed to the organic Zn and not to the methionine component. Zn retention and protein utilization were also improved by the Zn-Met supplement.
A preliminary study on the effect of adding yeast extract to cheese curd on proteolysis and flavour development of reduced-fat Cheddar
Authors: Shakeel-Ur-Rehman  , Farkye Nana Y , Vedamuthu Ebenezer R , Drake Mary A
Published: February 2003

Yeast extract was used as a nutrient for growing lactobacilli in reduced-fat Cheddar cheese as early growth of non-starter lactic acid bacteria (NSLAB) in Cheddar cheese is suppressed by pasteurization of milk and the hostile environment of the cheese. Reduced-fat Cheddar cheese was manufactured from 100 kg standardized milk on two occasions. After milling, the curd was divided into two portions, C and E. To control portion, C, salt was added at normal levels. A mixture of salt and yeast extract was added to the experimental, E. The cheeses were ripened for 7 months at 8 °C and assessed for proteolysis and NSLAB growth during ripening. Mean % moisture, fat, protein, salt and pH were 40·6, 20·5, 31·1, 1·72 and 5·22 respectively, in E cheeses, and 39·5, 20·5, 30·9, 1·68 and 5·22, respectively, in C cheese. NSLAB counts in E cheeses were 101, 103, 105 cfu/g compared with 0, 101, 104 cfu/g in C respectively, after 1, 7 and 30 d of ripening. After 60 d, cell densities of NSLAB were similar (∼106 cfu/g) in C and E cheese. Addition of yeast extract to curd affected neither the electrophoretic patterns of cheese nor its water-soluble N content during ripening. However, the total free amino acids were significantly higher in E cheese than C cheese throughout ripening, suggesting faster secondary proteolysis in the former cheeses. A 6-member trained descriptive panel evaluated the cheese at 7 months and found that the E cheeses had higher intensities of whey, fruity, sulphur, nutty, sweet and sour flavours, but had lower intensities of brothy flavours as compared to C cheeses. Also, the E cheeses were perceived to be more mature than corresponding C cheese. Results show that addition of yeast extract to cheese curd is a promising method of enhancing flavour development in ripened cheeses.
Use of ultrasonography to estimate cistern size and milk storage at different milking intervals in the udder of dairy cows
Authors: Ayadi Moez , Caja Gerardo , Such Xavier , Knight Christopher H
Published: February 2003

Four lactating Holstein cows (average milk yield: 20±3 l/d) were used to develop and validate a method for estimating the size of udder cisterns (Sinus lactiferi) using ultrasonography. A sectorial transducer probe of 5 MHz, placed in contact with the teat in a parallel cranial position, was used to obtain vertical scans of the udder in two perpendicular planes with the teat canal axis as reference. Udder scans for each udder quarter were taken randomly at intervals of 4, 8, 12, 16, 20 and 24 h after milking. Glandular parenchyma (echogenic) and lumen of the cisterns full of milk (anechogenic) were evident in the scans, the calculated area of the anechogenic portion being defined as cistern area. Cistern areas measured in perpendicular scans were highly correlated. Immediately after each measurement, cisternal milk was removed from each quarter using a teat cannula after i.v. injection of an oxytocin-receptor blocking agent. Alveolar milk from each quarter was then obtained by machine milking after i.m. injection of oxytocin. Cistern area and cisternal milk volume increased with length of milking interval showing a curvilinear pattern with a plateau after 16 h. Correlations between cistern area and cisternal milk volume were positive and significant (P<0·001) at all intervals but showed the highest values with the smallest residual standard deviations at 8 h (r=0·88) and 12 h (r=0·84). Since 8 h has previously been identified as a suitable time at which to determine cisternal milk volume for the purposes of defining suitability for different milking strategies, we conclude that ultrasonography provides a satisfactory, non-invasive method for determination of milk storage characteristics in dairy cows.