bluuebunny/crossref_metadata_2025 huggingface dataset:
PERAMALAN PENDAPATAN ASLI DAERAH MENGGUNAKAN METODE EXPONENTIAL SMOOTHING DENGAN PENDEKATAN STATE SPACE
Authors: Gumgum Gumgum Darmawan
Published: March 2024

Pendapatan Asli Daerah (PAD) merupakan dana yang diperoleh daerah antara lain dari hasil pajak, retribusi, pengelolaan kekayaan daerah, dan pendapatan daerah lain yang sah. PAD berfungsi untuk melihat gambaran keberhasilan pembangunan daerah dan secara langsung akan digunakan kembali untuk membiayai pembangunan daerah. Pada tahun 2021, Bappeda Kabupaten Cianjur menyusun draft RPJMD yaitu rancangan rencana pembangunan daerah selama 5 tahun ke depan yang di dalamnya berisi proyeksi anggaran dana Kabupaten Cianjur. Pada draft tersebut angka proyeksi PAD sangat tinggi sehingga menimbulkan kekhawatiran akan terlaksananya kegiatan pembangunan yang sudah direncanakan. Maka dilakukan peramalan PAD dengan metode statistika untuk membantu menyusun perencanaan yang tepat. Dalam melakukan peramalan, diperlukan metode yang sesuai sehingga dapat memberikan peramalan yang akurat. Pada penelitian ini ukuran data penelitian tidak banyak (n=29) dan data mengandung pola. Oleh karena itu penelitian ini menggunakan metode exponential smoothing dengan pendekatan model state space yang mana merupakan hasil dari pengembangan dari metode pemulusan eksponensial standar dengan menggunakan pendekatan model state space yang memperhatikan aspek level, tren, musiman, serta error sehingga model yang dihasilkan untuk melakukan peramalan lebih sesuai dengan data penelitian menghasilkan nilai akurasi prediksi yang lebih baik. Berdasarkan hasil analisis diperoleh model terbaik yang digunakan adalah model ETS(M,A,N) dan dihasilkan nilai MAPE sebesar 12,0119%.
PENINGKATAN DAYA TETAS TELUR DAN KELANGSUNGAN HIDUP IKAN PAPUYU (Anabas testudineus Bloch) PADA SISTEM CORONG DENGAN DEBIT ALIRAN BERBEDA
Authors: Ansyari Pahmi , Slamat Slamat
Published: March 2024

Ikan papuyu merupakan ikan ekonomis penting, sehingga budidayanya sudah cukup berkembang.  Masalahnya para pembudidaya sering kekurangan benih ikan papuyu, sehingga perlua adanya usaha peningkatan produksi benih, di mana salah satunya adalah dengan pembenihan sistem corong.  Penelitian ini bertujuan untuk mengetahui debit air optimal untuk mendapatkan daya tetas telur dan tingkat kelangsungan hidup larva ikan papuyu yang ditetaskan dengan sistem corong.  Desain penelitian adalah Rancangan Acak Lengkap (RAL) dengan 4 perlakuan dan 3 ulangan.  Perlakuan yang diterapkan adalah Perlakuan O = tanpa aliran (debit aliran 0 L/menit), A = 0,001 L/detik, B = 0,002 L/detik dan C = 0,003 L/detik.  Hasil penelitian menunjukkan daya tetas telur pada perlakuan O = 56,33%, A  = 76,76; B = 84,40% dan C = 80,34%, sedangkan tingkat kelangsungan hidup larva, perlakuan O = 73,44%, A = 73,22%, B = 74,45% dan C = 79,22%.  Daya tetas telur optimal pada debit aliran 0,002 L/detik, sedangkan tingkat kelangsungan hidup optimal pada debit aliran 0,003 L/detik
HUBUNGAN POLA MAKAN TERHADAP IMT DAN DISMINORHEA
Authors: Muzayyaroh  , Suyati
Published: March 2024

Remaja (mahasisiwi) adalah seseorang yang tumbuh menjadi dewasa dengan kematangan mental, emosional, sosial dan fisik. Remaja putri sangat memperhatikan bentuk citra tubuhnya sehingga seringkali melakukan diet ketat tanpa memperhatikan pola makan yang baik. Dengan fenomena tersebut maka banyak remaja putri yang memiliki berat bandan di bawah normal atau underweight. Pola makan yang buruk juga bisa memepengaruhi Kesehatan reproduksi khususnya saat menstruasi lebih mengalami banyak gangguan seperti Disminorhea atau nyeri hadi.

Penelitian ini menggunakan desain penelitian survey analitik dengan pendekatan Cross Sectional. Penelitian bertujuan untuk mengetahui hubungan pola makan dengan indek massa tubuh (IMT) dan Disminorhe pada mahasiswi D-III Kebidanan FIK Unipdu Jombang. Teknik pengumpulan data dalam penelitian ini dengan membagikan lembar informed consent, biodata diri dan  membagikan lembar kuesioner tentang pola makan dan disminorhe dengan menggunakan google form. Jenis data yang diambil adalah data primer, kemudian data dianalisa univariat dan bivariat menggunakan perangkat lunak computer dengan program Statistical Produk and Service Solution (SPSS) dan dianalisa dengan Teknik perhitungan statistic Chi Square. Penelitian ini dilaksanakan pada bulan September – November 2023 dengan populasi nya adalah mahasiswi D-III Kebidanan FIK Unipdu Jombang sejumlah 29 orang.

Hasil dari penelitian ini adalah adanya hubungan antara pola makan dengan IMT dan adanya hubungan antara pola makan dengan Disminorhea.

Dari penelitian dapat di simpulkan bahwa masih banyaknya mahasiswi prodi D-III Kebidanan FIK Unipdu yang mempunyai pola makan yang buruk dengan IMT underweight dan mengalami Dismonorhea
MAKNA PENGGUNAAN FIGUR PEREMPUAN DALAM KAIN BATIK KONTEMPORER
Authors: Prabowo Levina , Harsono Kelly , Oktaviani Lutfiah
Published: March 2024

Batik adalah satu dari banyaknya warisan budaya asli Indonesia yang telah dikenal di kancah dunia, bahkan UNESCO telah menetapkan Batik sebagai Intangible Cultural Heritage (ICH) atau Warisan Budaya Takbenda. Batik sendiri memiliki beragam jenis dan motifnya masing-masing. Namun sayangnya masih banyak jenis dan motif dari batik yang belum dikenal dan diketahui oleh masyarakat Indonesia. Metode penelitian yang digunakan untuk melakukan penelitian makna penggunaan figur perempuan dalam kain batik kontemporer ini adalah metode deskriptif kualitatif. Penelitian ini dilakukan dengan tujuan untuk mengungkapkan salah satu motif batik, yaitu motif figur perempuan yang divisualisasikan dalam kain batik kontemporer. Hasil penelitian yang dilakukan menunjukkan bahwa figur perempuan telah menjadi salah satu objek penting dan sumber inspirasi dari pembuatan seni batik. Penggunaan figur perempuan dalam kain batik biasanya berdasarkan cerita atau kisah yang ingin diangkat dalam seni batik tersebut. Figur perempuan dipilih karena merupakan tokoh atau bagian penting dari suatu cerita yang diangkat. Selain itu penggunaan figur perempuan dapat merepresentasikan banyak orang. Sehingga penelitian ini dapat menjadi salah satu bentuk upaya untuk menjaga dan melestarikan warisan  budaya batik, yang merupakan budaya Indonesia.
VISUALISASI BATIK PARANG YOGYAKARTA
Authors: Bagu Stevani Precious Vallerie , Tanumihardja Ninna Amadea , Michelle Michelle
Published: March 2024

Batik Parang merupakan motif batik khas Indonesia yang memiliki sejarah panjang. motif ini menampilkan pola geometris yang terinspirasi dari bentuk pisau Parang yang digunakan dalam tradisi Jawa. Sejarah Batik Parang dimulai pada masa Kerajaan Mataram pada abad ke-8, dimana batik menjadi bagian tak terpisahkan dari budaya Jawa. Motif Parang kemudian berkembang menjadi simbol keberanian dan kekuatan spiritual. Di dalam batik parang terdapat pengaruh perkembangan Hindu-Budha yang mengandung nilai nilai estetika. Batik merupakan identitas Indonesia yang mendapat pengakuan dari berbagai negara diluar Indonesia. Penelitian ini bertujuan untuk mengungkapkan sejarah batik parang  yang tidak banyak diketahui oleh masyarakat luas. Metode kualitatif dipilih dalam penelitian ini dan didukung dengan studi literatur. Dengan inovasi warna dan teknik desain, batik parang terus menjadi warisan budaya yang berharga. Seiring berjalannya waktu, batik ini tidak hanya dibuat dengan cara tradisional tetapi juga dibuat dengan cara modern. Maka dari itu, batik parang tetap mempunyai arti penting dalam mewujudkan keindahan dan kekayaan budaya Indonesia. Penelitian ini diharapkan bisa memberikan pengetahuan dan pemahaman kepada masyarakat luas tentang cara-cara baru untuk mengembangkan batik motif parang.
KAJIAN SEMANTIK PENAMAAN KAFE DI WATAMPONE KABUPATEN BONE
Authors: Eva Fajriani  , A. Muh. Taufiq  , Irna Fitriana
Published: March 2024

Penelitian ini dilakukan pada bulan Juli sampai Agustus 2023. Penelitian ini merupakan penelitian deskriptif semantik. Objek penelitian adalah 25 kafe yang terdapat di Watampone Kabupaten Bine. Proses penelitian dilaksanan dengan menggunakan metode wawancara dan dokumentasi. Dari hasil penelitian yang penulis lakukan menunjukkan bahwa terdapat beberapa variasi makna pada nama-nama  kafe yang terdapat di Watampone yang dibagi kedalam beberapa jenis yakni: (1)  makna denotasi (2) makna konotasi, (3) makna grammatikal, dan (4)  makna asosiasi yang meliputi. Adapun variasi nama-nama kafe yang terdapat di Watampone yang berdasarkan latar belakang penamaannya yang meliputi: (1) penamaan berdasarkan sifat khas, (2) penyebutan bagian, (3) nama pemilik/pembuat, (4) penamaan baru atau penggantian, (5) tempat asal, dan (6) pemendekan.
PENGARUH PEMBELAJARAN BLENDED LEARNING TERHADAP KEMANDIRIAN BELAJAR SISWA KELAS X SMK NEGERI 2 BONE
Authors: Selpi  , Suhardiman  , Ahmad Nurul Ihsan B
Published: March 2024

This research aims to determine the influence of Blended Learning on student self-directed learning in Grade X of SMK Negeri 2 Bone. The research was conducted from July to August 2023. This study is a quantitative descriptive research. The research population consisted of 304 students in Grade X of DKV at SMK Negeri 2 Bone, with a sample of 55 students. Data collection in the research process was carried out using questionnaires and documentation. Based on the research results, it is found that the respondents' perceptions of Blended Learning in relation to student self-directed learning amounted to 75%. This indicates that the respondents' opinions fall within the "agreeable," "good," or "likable" category because 75% falls within the range of 60% - 79.99%. Furthermore, the additional results of the t-test show that the calculated t-value is 0.625, which is smaller than the tabulated t-value of 2.006, and the significance value is 0.535, which is greater than 0.05. This suggests that the null hypothesis (Ho) is accepted, and the alternative hypothesis (H1) is rejected. Therefore, it can be concluded that Blended Learning has a non-significant influence on student self-directed learning.
Characteristics analysis of integrated science question items with the rasch model
Authors: Yamtinah Sri , Ashadi Ashadi , Mulyani Bakti , Utami Budi , Kristyasari Marantika Lia
Published: March 2024

This study aims to analyze the characteristics of the Integrated Science question item. This is quantitative descriptive research. The research subjects were 90 students of class VIII Junior High School in the high, medium, and low categories in Surakarta City. The characteristics of the items were analyzed using the Winstep program with the Rasch Model. Based on data analysis, the results obtained are: 1) the validity of conformity level of the items is valid; 2) the reliability value of the students was 0,79 which was sufficient, the reliability of the items was 0,93 which was very good, and the reliability between the students and the items (Alpha Cronbach) was 0,81 which was very good; 3) the value of separation item > value separation of students; 4) the distribution of the difficulty level of the questions is generally in the medium category; and 5) the difference power of the questions in the very good category is 35%, 25% in the good, 30% in the moderate, and 10% in the unable to distinguish category. Thus, it can be concluded that the analysis of the characteristics of the Integrated Science question items using the Rasch Model is considered good.
Environmental literacy portrait of pre-service science teacher in Indonesia
Authors: Vilmala Berry Kurnia , Suhandi Andi , Permanasari Anna , Kaniawati Ida , Purwanto Hadi
Published: March 2024

This article aims to picture the environmental literacy of the pre-service science teachers at Universitas Muhammadiyah Riau (UMRI). The highlighted environmental literacy consists of aspects of knowledge, attitudes and behavior of students related to environmental issues which are also part of the two themes of Educational for Sustainable Development (ESD), namely the theme of water and the theme of energy. Data were obtained through validated test items and questionnaire instruments. The results of this study obtained that students' environmental literacy was in the sufficient category, with an overall average percentage of environmental literacy for prospective science teacher students of 59.19. In addition, it was also obtained that the average percentage of environmental literacy of female students for the three aspects was higher than that of male students, although statistically it was concluded that there was no difference in environmental literacy of male students compared to female students. These results indicate that it is necessary to innovate lectures so that students' environmental literacy increases. One of the potential courses is Environmental Education which can be implemented on a project basis in accordance with the issues or themes in ESD.
The importance of green education in primary, secondary and higher education: A review
Authors: Adnyana I Made Dwi Mertha , Mahendra Kadek Adi , Raza Syed Meesam
Published: March 2024

This review aimed to provide an understanding of the importance of implementing green education in primary, secondary, and higher education. Green education is important in primary, secondary, and higher education because it promotes environmental conservation and sustainability. It integrates various disciplines and fosters ecological awareness, responsible citizenship, and sustainable practices. Green education in primary education introduces basic environmental concepts, whereas secondary education delves deeper into environmental issues and emphasizes critical thinking. Higher education provides specialized programs and courses in environmental studies, sustainability science, and green technology, equipping students with the expertise necessary to address global environmental challenges. Green education facilitates evidence-based learning, enabling individuals to make informed decisions and take action toward a more sustainable world.
Implementasi Strategi Pembelajaran Berorientasi STEM Terhadap Kemampuan Literasi Sains pada Siswa SD
Authors: Listiyana Asmi , Bima Muhammad Zidan Arya , Khusna Nashikhatul , Dewi Pambayun Wardani Candra , Putri Shianindra Rahmani , Yuniarti Yeni , Sukardi Rendi Restiana
Published: March 2024

Abstract: This study aimed to determine the effect of the application of STEM-oriented learning on science literacy skills in elementary school students. The research type was quasi-experimental. The population of this study was all students of SD Negeri Mungkid 2. The sample of this study was all grade IV students of SD Negeri Mungkid 2, totaling 16 people. The research sample was obtained through a purposive sampling process. The results of students' science literacy ability data were obtained through pretest and posttest. The data were analyzed using SPSS Statistics 20. Data processing began with normality test, paired simple t test, hypothesis test, and N-gain test. The results showed that the implementation of STEM-oriented learning was proven to affect science literacy skills in elementary school students.
Abstrak: Penelitian ini bertujuan untuk mengetahui pengaruh penerapan pembelajaran berorientasi STEM terhadap kemampuan literasi sains pada siswa sekolah dasar. Penelitian ini merupakan jenis penelitian eksperimen semu (quasi experimental). Populasi penelitian ini adalah seluruh siswa SD Negeri Mungkid 2. Sampel penelitian ini adalah seluruh siswa kelas IV SD Negeri Mungkid 2 yang berjumlah 16 orang. Sampel penelitian didapatkan melalui proses purposive sampling. Hasil data kemampuan literasi sains siswa didapat melalui kegiatan pretest dan posttest. Data dianalisis menggunakan SPSS Statistik  20. Pengolahan data dimulai dengan uji normalitas, uji paired simple t test, uji hipotesis, dan uji N-gain. Hasil penelitian menunjukkan bahwa implementasi pembelajaran berorientasi STEM terbukti berpengaruh terhadap kemampuan literasi sains pada siswa sekolah dasar.
Analisis Nilai Karakter pada Buku Cerita Rakyat Si Kancil Disusun untuk Memenuhi Tugas Mata Kuliah Menulis Artikel Hasil Penelitian
Authors: Juniarti Tri Mugni , Salshabila Lucyanna , Kaltsum Labibah Salwa , Kistianingrum Vershanda Dwi , Setianingrum Fitri , Sukardi Rendi Restiana , Yuniarti Yeni
Published: March 2024

Tujuan dari penelitian yang dilakukan yaitu untuk mendeskripsikan dan menganalisis nilai-nilai Pendidikan karakter yang terkandung pada buku cerita rakyat si kancil. Mengingat pentingnya Pendidikan karakter bagi peserta didik maka peneliti mengemas beberapa jenis karakter sesuai dengan kurikulum merdeka yang dijadikan sebagai acuan untuk menganalisis beberapa pendidikan karakter yang ada pada buku cerita rakyat si kancil. Jenis metode penelitian yang digunakan dalam penelitian ini yaitu penelitian kualitatif. Teknik pengumpulan data yang digunakan dalam penelitian ini yaitu analisi dokumen yang disajikan dalam bentuk tabel analisis nilai karakter pada buku cerita si kancil. Berdasarkan hasil penelitian yang telah dilakukan beberapa nilai karakter yang terkandung dalam buku cerita si kancil yaitu religious, nasionalis, gotong royong, integritas. Nilai karakter tersebut dibuktikan dengan adanya beberapa percakapan dan juga tindakan yang dilakukan pada cerita si kancil.
“Svanen fra Tuonela”
Authors: Daniels Beck Bolette , Lindvang Charlotte , Kolbe Krøier Julie
Published: March 2024

I vores samfund må vi ikke være ulykkelige, svage eller lammede af sorg. Der er opstået en skærende kontrast mellem accelererende krav til personlig performance og effektivitet og det menneskelige behov for at blive rummet og grebet, når tab og kriser rammer. I denne artikel beskrives en særlig form for sorgpraksis, der bygger på musikkens potentiale til at skabe rum for at være ved, udforske og dele følelser og indre oplevelser relateret til forskellige typer af sorg. Artiklens forfattere praktiserer og forsker i den musikterapeutiske metode Guided Imagery and Music (GIM). GIM bygger på musiklytning og indre billeddannelse til særligt udvalgte klassiske stykker musik og i forskellige vignetter og to fyldige caseberetninger præsenterer vi betydningen af at validere, acceptere og være med sorgen. Et GIM-forløb som terapeutisk og kreativ sorgpraksis er en transformerende rejse, som kan åbne til et udvidet menneskeligt rum med plads til sorg og smerte, og som samtidigt kan vise vej til nyorientering og håb (med reference til tosporsmodellen). Artiklen uddyber hvordan musik som en kunstform, der udstrækkes i tid, kan spejle og favne den sorg, som ellers kan være vanskelig at udtrykke i ord.
From Humor to Strategy: An Experimental Survey on Internet Memes in Social Media Marketing
Authors:   Faculty of Management, University of Warsaw, Poland, Kiljańczyk Mateusz , Kacprzak Agnieszka ,   Faculty of Management, University of Warsaw, Poland
Published: March 2024

Purpose: The paper addresses the issue of the use of Internet memes as a marketing communication tool in social media. The effectiveness of this type of communication is an important issue due to the large role of memes in digital culture, and at the same time the small number of studies on the use of memes in marketing communication. Design/methodology/approach: To verify the research hypotheses, a survey with an experimental design was conducted among 153 respondents. For the purposes of the study, effectiveness was understood as the ability of a message to induce the passage of the recipient through all stages of perception of the persuasive message, with the last stage being the acceptance of the sender’s outlook. Findings: The results of the study allowed us to draw conclusions about greater effectiveness of marketing communication using Internet memes in comparison to marketing communication without memes. The level of consumer interest in Internet memes turned out to be a differentiating factor in the perception of the form of marketing communication. Research limitations/implications: The main limitation of the study is the non-representativeness of the research sample. The practical implications of the study include guidance on the use of memes when conducting marketing communication on the Internet, taking into account preferences of the target group. Originality/value: This paper not only contributes importantly to the limited literature on Internet memes in marketing activities, but also turns the spotlight onto the characteristics of consumers who are the potential target group of such communication.
Collaborative Performances of Wine Tourism Destinations in the Northern Rhone Valley
Authors:   Department of Tourism and Recreation Faculty of Physical Education and Physiotherapy, Opole University of Technology, Poland, Hołodnik Daria
Published: March 2024

Purpose: The main aim of this article was to identify the wine tourism destination models set up on collaborative performances driven by Tourism Offices (Destination Marketing Organization, DMO) and wineries (private enterprises) from the appellate areas in the Northen Rhone Valley. Design/methodology/approach: The ethnographical methodology was used, including methods of participating observation (applied by a mysterious tourist method), study tours (to explore the study field), expert analysis (based on knowledge exchange with Tourism Office Managers) and official interviews (with Mangers of small and large wine producers from the areas). Findings: Two of the three surveyed wine tourism destinations from the Northern Rhône Valley (Vienne Condrieu and Rhône Crussol) are distinguished by the very active role of tourism organizations in building collaborative wine tourism offerings, in particular with small wineries. The third destination surveyed (Ardèche Hermitage) is more oriented toward collaborations with large wine producers and their independent role in leading wine tourism marketing. Research limitations/implications: Managers of tourism organizations should be oriented toward collaboration with both: large and small wine producers. Large wineries are helpful in raising the visibility and image of a wine destination. Small producers, on the other hand, offer a distinctive and individual wine tourism experience. Originality/value: The key aspect of wine tourism marketing is the building of collaborative wine tourism offerings led by DMO’s collaboration with both large and small wineries from the destination.
Redesigning the Employer Value Proposition in Times of Crisis
Authors:   D epartment of Marketing and Communication, Faculty of Management, Czestochowa University of Technology, Poland, Łazorko Katarzyna
Published: March 2024

Purpose: The purpose of the paper was to explore how the largest companies in Poland responded to the changing socio-political situation (the COVID-19 pandemic, the war in Ukraine, the inflation) in their employer branding communication, with potential candidates for jobs, especially in the aspect of their Employer Value Proposition (EVP) building. Methodology: The research examined whether the 50 biggest Polish enterprises changed their external employer branding communication via their web pages between 2019 and 2023. In the study, the online content analysis technique was used to review “Career” and relevant web pages. Findings: The presentation of changing expectations of candidates, as well as analysis of the content of the entrepreneurs’ web pages lead to the conclusion that not many Polish enterprises have changed their Employer Value Proposition (EVP) in the last four years to adjust to expectations of potential employees (about 30% in the sample), although, due to their scale of operation their marketing budgets are probably higher than in the case of the rest of the market. There may be many reasons behind that situation, including the specificity of different industries, as well as unpredictability, and suddenness of changes in their environment. The expectations of employees have changed significantly in the last four years, due to COVID-19 and some political and economic occurrences, and the process has not finished yet. Consequently, the situation in the labor market is dynamic, although, as observed, some companies react expeditiously to meet the expectations of potential employees. Research limitations: In the case of the study, the employer branding communication channels analysis was limited only to web pages and communication with external audiences, although, web pages and the “Career” bookmarks are indicated by Polish employers as one of the most frequently used tools to communicate with candidates. Another problem can be identified when any overall assumptions are made in the area of employer branding. The companies chosen for the study operate in different sectors and the specification of their performance and situation in the labor markets they target is different. Originality/value: The paper contributes to the discussion on employer branding in Poland. The topic is extensive and provides many opportunities for analyses conducted from various perspectives.
Understanding the Role of Sociocultural Influences on Entrepreneurial Activities: a Study of Ukrainian Women War Refugees in Poland
Authors:   Institute of Management, University of Szczecin, Poland, Gadomska-Lila Katarzyna , Ścibior-Butrym Anna ,   Doctoral School of the University of Szczecin, Institute of Management, Poland
Published: March 2024

Purpose: The aim of the article is to identify the socio-cultural determinants of entrepreneurial activities of Ukrainian refugee women in Poland. The manuscript points out the complexity of the phenomenon of entrepreneurship as an important element of economic adaptation of women experiencing migration and presents the results of empirical research. Design/methodology/approach: the research conducted was exploratory in nature, carried out using qualitative methods. Two focus group interviews were conducted with 16 female respondents. Findings: The analysis of the collected research material reveals the multidimensionality of the socio- -cultural aspects of the entrepreneurial activities of Ukrainian refugee women in Poland, particularly highlighting such factors as the value system, entrepreneurial traditions, risk propensity, social relations and entrepreneurial competences. Research limitations/implications: despite significant findings regarding the importance of socio-cultural determinants of the development of entrepreneurial activities of Ukrainian refugee women in Poland, the presentation of the perspective of one group, as well as the cultural context, may be a limitation of the research conducted. Originality/value: The data obtained were classified into groups of factors stimulating and blocking the entrepreneurship of Ukrainian refugee women. In addition, the results of the research made it possible to present a fairly wide spectrum of pro-entrepreneurial initiatives that can provide recommendations for aid organisations, institutions working for the adaptation of persons in a migratory situation, as well as employers who employ these persons
Structural constraints on the emergence of oscillations in multi-population neural networks
Authors: Zang Jie , Liu Shenquan , Helson Pascal , Kumar Arvind
Published: March 2024

Oscillations arise in many real-world systems and are associated with both functional and dysfunctional states. Whether a network can oscillate can be estimated if we know the strength of interaction between nodes. But in real-world networks (in particular in biological networks) it is usually not possible to know the exact connection weights. Therefore, it is important to determine the structural properties of a network necessary to generate oscillations. Here, we provide a proof that uses dynamical system theory to prove that an odd number of inhibitory nodes and strong enough connections are necessary to generate oscillations in a single cycle threshold-linear network. We illustrate these analytical results in a biologically plausible network with either firing-rate based or spiking neurons. Our work provides structural properties necessary to generate oscillations in a network. We use this knowledge to reconcile recent experimental findings about oscillations in basal ganglia with classical findings.
Learned response dynamics reflect stimulus timing and encode temporal expectation violations in superficial layers of mouse V1
Authors: Knudstrup Scott G , Martinez Catalina , Gavornik Jeffrey P
Published: March 2024

The ability to recognize ordered event sequences is a fundamental component of sensory cognition and underlies the capacity to generate temporally specific expectations of future events based on previous experience. Various lines of evidence suggest that the primary visual cortex participates in some form of predictive processing, though many details remain ambiguous. Here we use two-photon calcium imaging in layer 2/3 (L2/3) of the mouse primary visual cortex (V1) to study changes in neural activity under a multi-day sequence learning paradigm with respect to prediction error responses, stimulus encoding, and time. We find increased neural activity at the time an expected, but omitted, stimulus would have occurred but no significant prediction error responses following an unexpected stimulus substitution. Sequence representations became sparser and less correlated with training, although these changes had no effect on decoding accuracy of stimulus identity or timing. Additionally, we find that experience modifies the temporal structure of stimulus responses to produce a bias towards predictive stimulus-locked activity. Finally, we observe significant temporal structure during intersequence rest periods that was largely unchanged by training.
Early steps of protein disaggregation by Hsp70 chaperone and class B J-domain proteins are shaped by Hsp110
Authors: Sztangierska Wiktoria , Wyszkowski Hubert , Pokornowska Maria , Kochanowicz Klaudia , Rychłowski Michal , Liberek Krzysztof , Kłosowska Agnieszka
Published: March 2024

Hsp70 is a key cellular system counteracting protein misfolding and aggregation, associated with stress, ageing, and disease. Hsp70 solubilises aggregates and aids protein refolding through substrate binding and release cycles regulated by co-chaperones: J-domain proteins (JDPs) and nucleotide exchange factors (NEFs). Here, we elucidate the collaborative impact of Hsp110 NEFs and different JDP classes throughout Hsp70-dependent aggregate processing. We show that Hsp110 plays a major role at initial stages of disaggregation, determining its final efficacy. The NEF catalyses the recruitment of thick Hsp70 assemblies onto aggregate surface, which modifies aggregates into smaller species more readily processed by chaperones. Hsp70 stimulation by Hsp110 is much stronger with class B than class A JDPs and requires the auxiliary interaction between class B JDP and the Hsp70 EEVD motif. Furthermore, we demonstrate for the first time that Hsp110 disrupts the JDP-Hsp70 interaction. Such destabilisation of chaperone complexes at the aggregate surface might improve disaggregation, but also lead to the inhibition above the sub-stoichiometric Hsp110 optimum. Thus, balanced interplay between the co-chaperones and Hsp70 is critical to unlock its disaggregating potential.
A single-cell atlas of E. faecalis wound infection reveals novel bacterial-host immunomodulatory mechanisms
Authors: Celik Cenk , Lee Stella Yue Ting , Tanoto Frederick Reinhart , Veleba Mark , Kline Kimberly A. , Thibault Guillaume
Published: March 2024

Wound infections are highly prevalent, and can lead to delayed or failed healing, causing significant morbidity and adverse economic impacts. These infections occur in various contexts, including diabetic foot ulcers, burns, and surgical sites.
Enterococcus faecalis
is often found in persistent non-healing wounds, but its contribution to chronic wounds remains understudied. To address this, we employed single-cell RNA sequencing (scRNA-seq) on infected wounds in comparison to uninfected wounds in a mouse model. Examining over 23,000 cells, we created a comprehensive single-cell atlas that captures the cellular and transcriptomic landscape of these wounds. Our analysis revealed unique transcriptional and metabolic alterations in infected wounds, elucidating the distinct molecular changes associated with bacterial infection compared to the normal wound healing process. We identified dysregulated keratinocyte and fibroblast transcriptomes in response to infection, jointly contributing to an anti-inflammatory environment. Notably,
E. faecalis
infection prompted a premature, incomplete epithelial-to-mesenchymal transition in keratinocytes. Additionally,
E. faecalis
infection modulated M2-like macrophage polarization by inhibiting pro-inflammatory resolution
in vitro
,
in vivo,
and in our scRNA-seq atlas. Furthermore, we discovered macrophage crosstalk with neutrophils, which regulates chemokine signaling pathways, while promoting anti-inflammatory interactions with endothelial cells. Overall, our findings offer new insights into the immunosuppressive role of
E. faecalis
in wound infections.Wound infections, including diabetic foot ulcers, burns, or surgical sites, often lead to prolonged healing and significant health and economic burdens. Among the bacteria implicated in these persistent wounds,
Enterococcus faecalis
remains a relatively enigmatic player. To unravel its role in non-healing wounds, we used single-cell RNA sequencing in a mouse model, scrutinizing over 23,000 cells to construct a comprehensive single-cell map of infected wounds compared to uninfected wounds. Our investigation revealed distinct genetic and metabolic alterations in infected wounds, in which infection resulted in a perturbed inflammatory environment delayed wound healing signatures. Specifically,
E. faecalis
infection induces a premature and incomplete transition in keratinocytes, impeding their healing function. Furthermore, infection influences the behavior of immune cells like macrophages, affecting the body’s response to the infection. These findings not only shed light on
E. faecalis
’s role in delayed wound healing but also offer potential avenues for future treatments, providing valuable insights into the challenging realm of wound infections.
SUMO’s intrinsically disordered N-terminus is an intramolecular inhibitor of SUMO - SIM interactions
Authors: Richter Sebastian M. , Jin Fan , Ritterhoff Tobias , Fergin Aleksandra , Maurer Eric , Frank Andrea , Hajnal Alex , Klevit Rachel , Gräter Frauke , Flotho Annette , Melchior Frauke
Published: March 2024

Small Ubiquitin-related modifiers of the SUMO family regulate thousands of proteins in eukaryotic cells. Many SUMO substrates, effectors and enzymes carry short motifs (SIMs) that mediate low affinity interactions with SUMO proteins. This raises the question how specificity is achieved in target selection, SUMO paralogue choice and SUMO-dependent interactions. A unique but poorly understood feature of SUMO proteins is their intrinsically disordered N-terminus. We reveal a function for N-termini of human,
C. elegans,
and yeast SUMO proteins as intramolecular inhibitors of SUMO-SIM interactions. Mutational analyses, NMR spectroscopy, and Molecular Dynamics simulations indicate that SUMO’s N-terminus can inhibit SIM binding by fast and fuzzy interactions with SUMO‘s core. Deletion of the
C. elegans
SUMO1 N-terminus leads to p53-dependent apoptosis during germline development, indicating an important role of SUMO’s N-termini in DNA damage repair. Our findings reveal a mechanism of disorder-based autoinhibition that contributes to the specificity of SUMOylation and SUMO-dependent interactions.
Dependence of nucleosome mechanical stability on DNA mismatches
Authors: Ngo Thuy TM , Liu Bailey , Wang Feng , Basu Aakash , Wu Carl , Ha Taekjip
Published: March 2024

The organization of nucleosomes into chromatin and their accessibility are shaped by local DNA mechanics. Conversely, nucleosome positions shape genetic variations, which may originate from mismatches during replication and chemical modification of DNA. To investigate how DNA mismatches affect the mechanical stability and the exposure of nucleosomal DNA, we used an optical trap combined with single-molecule FRET and a single-molecule FRET cyclization assay. We found that a single base-pair C-C mismatch enhances DNA bendability and nucleosome mechanical stability for the 601-nucleosome positioning sequence. An increase in force required for DNA unwrapping from the histone core is observed for single base-pair C-C mismatches placed at three tested positions: at the inner turn, at the outer turn, or at the junction of the inner and outer turn of the nucleosome. The results support a model where nucleosomal DNA accessibility is reduced by mismatches, potentially explaining the preferred accumulation of single-nucleotide substitutions in the nucleosome core and serving as the source of genetic variation during evolution and cancer progression. Mechanical stability of an intact nucleosome, that is mismatch-free, is also dependent on the species as we find that yeast nucleosomes are mechanically less stable and more symmetrical in the outer turn unwrapping compared to Xenopus nucleosomes.
Bacterial exonuclease III expands its enzymatic activities on single-stranded DNA
Authors: Wang Hao , Ye Chen , Lu Qi , Jiang Zhijie , Jiang Chao , Zhou Chun , Li Na , Zhang Caiqiao , Zhao Guoping , Yue Min , Li Yan
Published: March 2024

Bacterial exonuclease III (ExoIII), widely acknowledged for specifically targeting double-stranded DNA (dsDNA), has been documented as a DNA repair-associated nuclease with apurinic/apyrimidinic (AP)-endonuclease and 3′→5′ exonuclease activities. Due to these enzymatic properties, ExoIII has been broadly applied in molecular biosensors. Here, we demonstrate that ExoIII (Escherichia coli) possesses highly active enzymatic activities on ssDNA. By using a range of ssDNA fluorescence-quenching reporters and fluorophore-labeled probes coupled with mass spectrometry analysis, we found ExoIII cleaved the ssDNA at 5′-bond of phosphodiester from 3′ to 5′ end by both exonuclease and endonuclease activities. Additional point mutation analysis identified the critical residues for the ssDNase action of ExoIII and suggested the activity shared the same active center with the dsDNA-targeted activities of ExoIII. Notably, ExoIII could also digest the dsDNA structures containing 3′-end ssDNA. Considering most ExoIII-assisted molecular biosensors require the involvement of single-stranded DNA (ssDNA) or nucleic acid aptamer containing ssDNA, the activity will lead to low efficiency or false positive outcome. Our study revealed the multi-enzymatic activity and the underlying molecular mechanism of ExoIII on ssDNA, illuminating novel insights for understanding its biological roles in DNA repair and the rational design of ExoIII-ssDNA involved diagnostics.
Gliogenesis from the subventricular zone modulates the extracellular matrix at the glial scar after brain ischemia
Authors: Ardaya Maria , Tiveron Marie-Catherine , Cremer Harold , Dehay Benjamin , Pérez-Cerdá Fernando , Matute Carlos , Soria Federico N. , Cavaliere Fabio
Published: March 2024

Activation of the subventricular zone (SVZ) after cerebral ischemia is one of the early responses in the brain to counteract the loss of neurons and reduce tissue damage. Impaired brain regions communicate with the SVZ through many chemotactic messages that result in neural stem cells (NSC) migration and differentiation. However, the activation of gliogenesis and the role of newborn astrocytes in the post-ischemic scenario is still under debate. We have previously shown that adenosine release after brain ischemia prompts the SVZ to generate new astrocytes. Here, we use transient brain ischemia in mice to define the cellular origin of these astrocytes in the SVZ neurogenic niche and investigate their role in the pathological process. By combining immunofluorescence, BrdU-tracing and genetic cellular labelling, we tracked the migration of newborn astrocytes, positive for the proteoglycan marker Thbs4, from the dorsal and medial SVZ to the perilesional barrier surrounding the ischemic core, termed “glial scar”. We found that these Thbs4-positive astrocytes modulate the dense extracellular matrix at the lesion border by synthesis but also degradation of hyaluronan. We also show that while the accumulation of this polymer at the lesion is sufficient to recruit newborn astrocytes, its degradation at the SVZ correlates with gliogenesis. These results point to newborn astrocytes as a plausible pharmacological target to modulate the glial scar after brain ischemia and facilitate tissue regeneration.
Sobre construcciones apositivas. Nombre, género y locación
Authors: Cabezas Holgado Emilio
Published: March 2024

Las construcciones apositivas se han descrito a partir de la tradición gramatical desde la perspectiva de la adyacencia nominal ‘especificativa’ o ‘explicativa’, incluyendo estructuras de composición léxica y/o naturaleza incidental y obviando otras que establecen relaciones de predicación propiamente ‘identificativas’ a partir de sentidos como el de ‘origen’ o ‘localización’. En este trabajo se revisará la gramática de las construcciones apositivas por medio de criterios léxico-sintácticos y discursivos, así como su clasificación, que presenta una clara vinculación estructural con los casos gramaticales nominativo, genitivo y locativo.
Facilities location for disaster response teams using GIS
Authors: Barino Raphael Sepulveda , Cunha Claudia Brito da , Solano Carlos Mario Diaz
Published: March 2024

The recurrence of disasters in Brazil, stemming from extreme weather events and poor urban management, exposes the population to calamitous situations caused by floods, landslides, and other adversities. In the face of these events, the need arises to establish a network of assistance, known as humanitarian logistics, which brings together participants at different levels to alleviate human suffering. However, the literature highlights gaps, such as the problematic location of facilities, asset-based solutions, partnerships, and collaboration. In this context, the Sistema S, a nationally recognized entity with social interest, emerges as a potential solution to address these gaps. This paper aimed to evaluate the asset infrastructure of Sistema S located in Nova Friburgo, a mountainous region in the state of Rio de Janeiro, to assess its feasibility for aiding victims and restoring interrupted daily and economic activities due to disasters. To conduct the study, a literature review was carried out to support the theme, followed by an analysis of the study area through Georeferencing using the QGIS software. The results indicate that, despite the privileged location and infrastructure with service capacity, its spatial integration is vulnerable to being affected by the region's climatic events.
Growth and Yields Performance Evaluation of Maize Raised on Termite Mound and Surrounding Soils
Authors: Malumi Barnabas Omolere , Dolapo Tawakalitu Abiola , Ojo Olumuyiwa Idowu
Published: March 2024

Termites are social insects and the mounds they develop are considered to be a waste. They fed on agricultural products and wooden structures and hence, they are considered to be destructive. The resultant mound soil is an agricultural soil which is rich in nutrients, but its usefulness in agriculture and construction industry had not been explored. Thus, the study evaluated the Growth and yields performance of maize raised on termite mound and surrounding soils in Owo, Ondo State, Nigeria. Maize was cultivated on the termite and surrounding soils as well as on their 50-50% mixture by weight and the data of the yields of maize was subjected to statistical analysis using T test at 95% conﬁdence level. The yields of maize cobs per stands for termite mound soil in plots 1, 4, 7 were 1,2,1; for the surrounding soil in plots 3,6,9 are 1,1,1 and for the mixture of the surrounding; and the termite mound soil were 3,2,2. The yields of maize cultivated on plots 1, 4, 7 (TMS); 2, 5, 8 (TMS+SRS); 3, 6, 9 (SRS) using t-distribution table were O.75<u<l .87 or l.31±O.56; l.68<u<2.92 or 2.30±0.62; 0.75<u<l.25 or 1.00±0.25. Mound soil had high nutrient content and high shear strength, thus it can be used for soil amendment. The findings from the study added to the knowledge database on soil fertility and can be adopted for similar work in any part of the country.
A Computational Approach on Fenofibrate Drug Using Degree-Based Topological Indices and M-polynomials
Authors: Nagarajan S. , Durga M.
Published: March 2024

Fenofibrate is a drug approved by FDA Food and Drugs Administration used to treat patients with Hypertrigly ceridemia primary hypercholesterolemia or mixed dyslipidemia. It reduces low-density lipoprotein cholesterol (LDL-c), total cholesterol, triglycerides, and apolipoprotein B and increases high -density lipoprotein cholesterol (HDL-C) in adults. The sum and multiplicative versions of several topological indices such as General Zagreb, General Randic, Arithmetic Geometric Index, Inverse sum (Indeg) Index, Symmetric Division (Deg) Index, Forgotten Indices M-polynomials of Fenofibrate are computed in this article.
Identification of Rhizosphere Endophytes and Evaluation of Their Impact on Growth of Chia (Salvia hispanica L.)
Authors: Sumarani D V  , Lakshmeesha R  , Harish J , Kumar K M Harini , Babu C V
Published: March 2024

Endophytic microorganisms promote root growth, improve nutrient uptake efficiency and produce more yields. By collaborating with endophytic microorganisms, plants experience enhanced growth. This study was focused on endophytes from Chia plants in Bhukkapatna, Tumakur with the objective of molecular characterization of efficient endophytic microorganisms and its impact on growth of Chia. Twelve bacterial isolates were identified, with eight displaying nitrogen fixation, phosphate solubilisation, potassium fixation, production of IAA, GA, siderophore and exopolysaccharide production. Molecular characterization using 16S rRNA revealed their identities as Pseudomonas fluorescens, Bacillus cereus, Stenotrophomonas maltophilia, and Enterobacter cloacae. Among these, Enterobacter cloacae (CEPB8) exhibited highest positive impact on Chia plant growth. In greenhouse conditions, treatments involving combinations of all four demonstrated the highest plant height (35.467cm) and biomass (158.37 grams) at harvest, surpassing the control group. Combined treatments outperformed individual strains, highlighting synergy. The study affirms that endophytic microorganisms significantly enhanced growth in Chia plant.
Application of Blockchain Technology to Reduce Costs
Authors: Al Chlaihawi Dr. Mohammed Oleiwi
Published: March 2024

The research aims to explain the cognitive foundations of Blockchain technology, clarify its types, characteristics, how it works, its advantages and disadvantages, clarify the applications of this technology in the Real Estate Registration Directorate and the implications of its application, and study and analyze the impact of the technology on reducing transaction costs by presenting a proposed model for application in the research sample unit. This technology is considered one of the technologies that is based on the idea of decentralization by eliminating the role of the intermediary, thus making transactions reliable, secure, and quick in completion, with the accompanying reduction of effort, time, and costs, as through it transactions can be completed in a few seconds or minutes, and do not require many Of the documents and documents that hold the unit responsible for providing a warehouse to store them and providing means of protection for their preservation, the researcher has reached a set of conclusions, the most important of which are: The application of Blockchain technology leads to reducing many procedures, which leads to a significant reduction in the costs of all these steps.
Asymmetric Catalytic [4+3] Cycloaddition Reactions
Authors: Harmata Michael
Published: November 2006

Although the [4+3] cycloaddition reaction has seen considerable attention over the past 10–15 years, only a few investigations have been dedicated to the development of a catalytic, asymmetric process. This short review covers some of the advances made in the area of absolute stereocontrol in this higher order cycloaddition and will hopefully serve as an inspiration towards the development of asymmetric, catalytic versions of the reaction.