bluuebunny/crossref_metadata_2025 huggingface dataset:
The Case for Long-Term Land Leasing: A Review of the Empirical Literature
Authors: Adenuga Adewale , Jack Claire , McCarry Ronan
Published: March 2021

Land, as a factor of production, has a vital role within the agricultural sector compared with other sectors. However, in recent years, land mobility has become a significant issue around the world with increased concentration and competition for land ownership, limiting the overall competitiveness of the agri-food sector and constraining the potential opportunities for new entrant farmers to access land. While land leasing is increasingly being embraced as a common form of land tenure serving as an alternative to the purchase of land for agriculture, the length of lease has been shown to have a significant impact on land productivity and sustainability. In this study, we provide a comprehensive and systematic review exploring the benefits of longer-term land leasing with a particular focus on developed countries and some selected developing countries in the context of commercial farming with more formal arrangements. Specifically, we highlight the barriers to long-term land leasing and identify potential incentives that might be adopted to encourage long-term land leasing for both landowners and farmers who seek to rent land.
Agricultural Technical Efficiency of Smallholder Farmers in Ethiopia: A Stochastic Frontier Approach
Authors: Zewdie Markose Chekol , Moretti Michele , Tenessa Daregot Berihun , Ayele Zemen Ayalew , Nyssen Jan , Tsegaye Enyew Adgo , Minale Amare Sewnet , Van Passel Steven
Published: March 2021

In the past decade, to improve crop production and productivity, Ethiopia has embarked on an ambitious irrigation farming expansion program and has introduced new large- and small-scale irrigation initiatives. However, in Ethiopia, poverty remains a challenge, and crop productivity per unit area of land is very low. Literature on the technical efficiency (TE) of large-scale and small-scale irrigation user farmers as compared to the non-user farmers in Ethiopia is also limited. Investigating smallholder farmers’ TE level and its principal determinants is very important to increase crop production and productivity and to improve smallholder farmers’ livelihood and food security. Using 1026 household-level cross-section data, this study adopts a technology flexible stochastic frontier approach to examine agricultural TE of large-scale irrigation users, small-scale irrigation users and non-user farmers in Ethiopia. The results indicate that, due to poor extension services and old-style agronomic practices, the mean TE of farmers is very low (44.33%), implying that there is a wider room for increasing crop production in the study areas through increasing the TE of smallholder farmers without additional investment in novel agricultural technologies. Results also show that large-scale irrigation user farmers (21.05%) are less technically efficient than small-scale irrigation user farmers (60.29%). However, improving irrigation infrastructure shifts the frontier up and has a positive impact on smallholder farmers’ output.
Transfer of Antibiotic Resistance Plasmid from Commensal E. coli towards Human Intestinal Microbiota in the M-SHIME: Effect of E. coli dosis, Human Individual and Antibiotic Use
Authors: Lambrecht Ellen , Van Coillie Els , Boon Nico , Heyndrickx Marc , Van de Wiele Tom
Published: March 2021

Along with (in)direct contact with animals and a contaminated environment, humans are exposed to antibiotic resistant bacteria by consumption of food. The implications of ingesting antibiotic resistant commensal bacteria are unknown, as dose-response data on resistance transfer and spreading in our gut is lacking. In this study, transfer of a resistance plasmid (IncF), harbouring several antibiotic resistance genes, from a commensal E. coli strain towards human intestinal microbiota was assessed using a Mucosal Simulator of the Human Intestinal Ecosystem (M-SHIME). More specifically, the effect of the initial E. coli plasmiddonor concentration (105 and 107 CFU/meal), antibiotic treatment (cefotaxime) and human individual (n = 6) on plasmid transfer towards lumen coliforms and anaerobes was determined. Transfer of the resistance plasmid to luminal coliforms and anaerobes was observed shortly after the donor strain arrived in the colon and was independent of the ingested dose. Transfer occurred in all six simulated colons and despite their unique microbial community composition, no differences could be detected in antibiotic resistance transfer rates between the simulated human colons. After 72 h, resistant coliform transconjugants levels ranged from 7.6 × 104 to 7.9 × 106 CFUcefotaxime resistant/mL colon lumen. Presence of the resistance plasmid was confirmed and quantified by PCR and qPCR. Cefotaxime treatment led to a significant reduction (85%) in resistant coliforms, however no significant effect on the total number of cultivable coliforms and anaerobes was observed.
Yttrium’s Effect on the Hot Cracking and Creep Properties of a Ni-Based Superalloy Built Up by Additive Manufacturing
Authors: Banoth Santhosh , Palleda Thaviti Naidu , Shimazu Sota , Kakehi Koji
Published: March 2021

We studied the effects of the rare earth element yttrium (Y) on the hot cracking and creep properties of Hastelloy-X processed by selective laser melting. We used two different alloys to study hot cracking in Hastelloy-X: one with 0.12 mass% yttrium added and one with no yttrium. Y-free Hastelloy-X exhibited less cracks, mainly due to the segregation of Si, W, and C resulting in SiC- and W6C-type carbides at the grain boundary and interdendritic regions. On the other hand, more cracks formed in the Y-added Hastelloy-X specimen because of segregation of Y, resulting in the formation of yttrium-rich carbide (YC). Post-heat treatment was conducted at 1177 °C for 2 h, followed by air cooling, to obtain good creep properties. We carried out a creep test along the vertical and horizontal directions. Despite having more cracks, the Y-added as-built Hastelloy-X specimen showed longer creep life and ductility than the Hastelloy-X specimen. This was mainly because of the formation of Y2O3 and SiO2 inside the grains. After solution treatment, the Y-added specimen’s creep life was eight times longer than that of the Y-free solution-treated specimen. This was mainly because of the maintenance of the columnar grain morphology even after solution treatment. In addition, the formation of M6C carbides, Y2O3, and SiO2 improved creep life. To summarize the effect of Y, Y addition promoted the formation of cracks, which brought about creep anisotropy; however, it improved creep properties through the stabilization of oxygen and the promotion of discrete carbide precipitation, which prohibited the migration and sliding of grain boundary.
Surface Analysis of TMCTS-Based SiOC(H) Low-k Dielectrics in Post-Etch Strip of ACL Hardmask
Authors: Park Min Kyu , Song Wan Soo , Kim Min Ho , Hong Sang Jeen
Published: March 2021

The miniaturization of devices requires the introduction of a high aspect ratio through patterning in the Damascene copper interconnect process. The high aspect ratio etch process employs hardmasks, such as amorphous carbon, that can withstand high-powered plasma exposure. When an etch hardmask is removed after patterning, the properties of the underlying film can be altered by the effect of plasma exposure during the strip process. In this study, surface properties of SiOC(H) are investigated after an amorphous carbon strip with O2/Ar plasma. Since the low-k film of SiOC(H) structure shows characteristics according to the Si-O internal bonding structure, the Si-O bonding ratio of the ring, network and cage structure was analyzed through Fourier-transform infrared (FT-IR) analysis to measure changes in thin film properties. X-ray photoelectron spectroscopy (XPS) was also used to add reliability to the SiOC(H) film structure. In addition, the end point of the strip process was obtained using an optical emission spectroscopy sensor and variations in thin film characteristics over the plasma exposure time were analyzed. These results revealed the structural modification of the SiCO(H) thin film in the post-etch strip of the amorphous carbon layer (ACL) hardmask.
The Influence of Starch Origin on the Properties of Starch Films: Packaging Performance
Authors: Żołek-Tryznowska Zuzanna , Kałuża Alicja
Published: March 2021

Starch films can be used as materials for food packaging purposes. The goal of this study is to compare how the starch origin influence the selected starch film properties. The films were made from various starches such as that from maize, potato, oat, rice, and tapioca using 50%w of glycerine as a plasticizer. The obtained starch-based films were made using the well-known casting method from a starch solution in water. The properties of the films that were evaluated were tensile strength, water vapour transition rate, moisture content, wettability, and their surface free energy. Surface free energy (SFE) and its polar and dispersive components were calculated using the Owens-Wendt-Rabel-Kaelbe approach. The values of SFE in the range of 51.64 to 70.81 mJ∙m−2 for the oat starch-based film and the maize starch-based film. The films revealed worse mechanical properties than those of conventional plastics for packaging purposes. The results indicated that the poorest tensile strength was exhibited by the starch-based films made from oat (0.36 MPa) and tapioca (0.78 MPa) and the greatest tensile strength (1.49 MPa) from potato.
Experimental Study on the Performance of Graded Glass Fiber Reinforced Concrete (G-GRC) Based on Engineering Application
Authors: Wang Qingbiao , Song Hongxu , Li Yue , Wang Fuqiang , Hu Zhongjing , Lou Shumei , Shi Zhenyue
Published: March 2021

An important way to improve concrete performance is the use of alkali-resistant glass fibers (ARGFs) as reinforcement. This paper is based on the problems of the cracking of the partition wall and lining seepage in Laoshan Tunnel, Qingdao, China. Two types of ARGFs were selected as reinforcement materials for the partition wall and lining concrete: high dispersion (HD) and high performance (HP); and the compressive strength (CS), tensile strength (TS), flexural strength (FS), and impervious performance (IP) of concrete with different gradations of the two types of fibers were investigated. The results show that although the CS of graded glass fiber reinforced concrete (G-GRC) is slightly decreased, the TS, FS, and IP of G-GRC are significantly improved. When the densities of the ARGFs of HD and HP are 0.6 and 5 kg/m3, respectively, G-GRC performs best; additionally, compared with ordinary concrete, the TS, FS, and IP of G-GRC are increased by 15.86%, 14.90%, and 31.58%, respectively. Meanwhile, the tension–compression ratio is increased by 22.29%, and the mechanical properties of concrete are remarkably enhanced. The research results were successfully applied to the construction of the Laoshan tunnel, and good engineering results were obtained.
Application of Teager–Kaiser’s Instantaneous Frequency for Detection of Delamination in FRP Composite Materials
Authors: Gałęzia Adam , Orłowska-Gałęzia Anita
Published: March 2021

Composite materials are widely used in many engineering applications and fields of technology. One of the main defects, which occur in fiber-reinforced composite materials, is delamination. It manifests itself in the separation of layers of material and the damaged structure once subjected to mechanical loads degrades further. Delamination results in lower stiffness and the decrease of structure’s carry load capability. Its early detection is one of the tasks of non-invasive structural health monitoring of layered composite materials. This publication discusses a new method for delamination detection in fiber-reinforced composite materials. The approach is based on analysis of energy signal, calculated with Teager–Kaiser energy operator, and comparison of change of the weighted instantaneous frequency for measurement points located in- and outside of delamination area. First, applicability of the developed method was tested using simple models of vibration signals, reflecting considered phenomena. Next, the authors’ weighted instantaneous frequency was applied for detection of deamination using signals obtained from FEM simulated response of the cantilever beam. Finally, the methods effectiveness were tested involving real experimental signals collected by the laser Doppler vibrometer (LVD) sensor measuring vibrations of the delaminated glass-epoxy specimens.
Experimental Investigation of Fatigue Crack Growth Behavior of the 2.25Cr1Mo0.25V Steel Welded Joint Used in Hydrogenation Reactors
Authors: Song Yan , Chai Mengyu , Han Zelin
Published: March 2021

In this work, the fatigue crack growth (FCG) behavior and fatigue damage mechanism of the 2.25Cr1Mo0.25V steel welded joint used in hydrogenation reactors were investigated. The multi-pass welding was carried out to manufacture the welded joint using the combined shielded metal arc welding (SMAW) and submerged automatic arc welding (SAAW) processes. The FCG behavior of different zones in the welded joint, including the base metal (BM), the heat-affected zone (HAZ) and the weld metal (WM), were studied by compact tension tests. Moreover, the acoustic emission (AE) technique was used to monitor AE signals generated from FCG process for further understanding FCG behavior and fatigue mechanisms. Additionally, the microstructures and fracture surfaces of different specimens were observed by optical microscopy (OM) and scanning electron microscopy (SEM). The results revealed that the microstructure of BM is fine granular bainite, while the WM shows coarser bainite grains. The HAZ exhibits the most significant inhomogeneity with large dispersion of grain size. FCG results showed that the HAZ exhibits much higher fatigue crack growth rate (FCGR) at low ΔK values, while the BM shows the most superior fatigue resistance. The AE technique is successful in monitoring and identifying damage evolutions during the FCG process. Moreover, an enhanced AE activity is observed in FCG of the WM specimen, which is attributed to the combined influence of the formation of numerous secondary cracks and coarse-grained microstructures.
Batch Reverse Osmosis Desalination Modeling under a Time-Dependent Pressure Profile
Authors: Chougradi Abdeljalil , Zaviska François , Abed Ahmed , Harmand Jérôme , Jellal Jamal-Eddine , Heran Marc
Published: March 2021

As world demand for clean water increases, reverse osmosis (RO) desalination has emerged as an attractive solution. Continuous RO is the most used desalination technology today. However, a new generation of configurations, working in unsteady-state feed concentration and pressure, have gained more attention recently, including the batch RO process. Our work presents a mathematical modeling for batch RO that offers the possibility of monitoring all variables of the process, including specific energy consumption, as a function of time and the recovery ratio. Validation is achieved by comparison with data from the experimental set-up and an existing model in the literature. Energetic comparison with continuous RO processes confirms that batch RO can be more energy efficient than can continuous RO, especially at a higher recovery ratio. It used, at recovery, 31% less energy for seawater and 19% less energy for brackish water. Modeling also proves that the batch RO process does not have to function under constant flux to deliver good energetic performance. In fact, under a linear pressure profile, batch RO can still deliver better energetic performance than can a continuous configuration. The parameters analysis shows that salinity, pump and energy recovery devices efficiencies are directly linked to the energy demand. While increasing feed volume has a limited effect after a certain volume due to dilution, it also shows, interestingly, a recovery ratio interval in which feed volume does not affect specific energy consumption.
Multicomponent Network Formation in Selective Layer of Composite Membrane for CO2 Separation
Authors: Lillepärg Jelena , Sperling Evgeni , Blanke Marit , Held Martin , Shishatskiy Sergey
Published: March 2021

As a promising material for CO2/N2 separation, PolyActiveTM can be used as a separation layer in thin-film composite membranes (TFCM). Prior studies focused on the modification of PolyActiveTM using low-molecular-weight additives. In this study, the effect of chemical crosslinking of reactive end-groups containing additives, forming networks within selective layers of the TFCM, has been studied. In order to understand the influence of a network embedded into a polymer matrix on the properties of the resulting materials, various characterization methods, including Fourier transform infrared spectroscopy (FTIR), gas transport measurements, differential scanning calorimetry (DSC) and atomic force microscopy (AFM), were used. The characterization of the resulting membrane regarding individual gas permeances by an in-house built “pressure increase” facility revealed a twofold increase in CO2 permeance, with insignificant losses in CO2/N2 selectivity.
Operation of Three-Stage Process of Lithium Recovery from Geothermal Brine: Simulation
Authors: Kalmykov Denis , Makaev Sergey , Golubev Georgy , Eremeev Ilia , Vasilevsky Vladimir , Song Jianfeng , He Tao , Volkov Alexey
Published: March 2021

Lithium-rich geothermal waters are considered as an alternative source, and further concentration of lithium is required for its effective recovery. In this work, we have simulated a three-stage lithium recovery process including the brine softening by precipitation Ca2+/Mg2+ cations with sodium carbonate (calculated in PHREEQC), followed by an integrated system consisting of membrane distillation unit (water evaporation), crystallizer (NaCl precipitation), and membrane extraction (Li+ recovery), which was simulated in Simulink/MATLAB. It was shown that the deterioration of membrane performance in time due to scaling/fouling plays a critical role in the performance of the system resulting in the dramatic increase of the replaced membrane modules by a factor of 5. Low cost membranes are required. The process simulation based on the experimental and literature data on the high salinity solutions with the membrane distillation revealed that the specific productivity can be achieved in the range of 9.9–880 g (Li+) per square meter of membranes in the module used before its replacement. The increase of energy efficiency is needed. The mass-flow-rate of saline solution circulated to the crystallizer was set at its almost minimum value as 6.5 kg/min to enable its successful operation at the given parameters of the membrane distillation unit. In other words, the operation of the integrated system having 140 kg of saline solution in the loop and a membrane module of 2.5 m2 for concentration of lithium presence from 0.11 up to 2.3 g/kg would be associated with the circulation of about of 259 tons of saline solution per month between the distillation unit (60 °C) and the crystallizer (15 °C) to yield of up to 1.4 kg of lithium ions. The comprehensive summary and discussion are presented in the conclusions section.
A Modified M-K Method for Accurate Prediction of FLC of Aluminum Alloy
Authors: Li Xiaoxing , Chen Yangkai , Lang Lihui , Xiao Rui
Published: March 2021

Forming limit curve (FLC) is an important failure criterion for sheet metals in sheet metal forming, while the M-K model is widely used for the prediction of the FLC. In the M-K model, such prediction is greatly influenced by the initial thickness imperfection factor and material properties, from which the original M-K model’s theoretical derivation is proposed as a solution to the above mentioned issue in this paper. Then the relationship between the M-K model and Keeler’s empirical formula is then studied, from which a new method to predict FLC is proposed that combines the M-K model with Keeler’s empirical formula according to the previous analyses. It turns out that this new method can simplify the calculation procedure. Finally, the experimental results of two kinds of aluminum alloys, AA6016 and AA5182, have verified the effectiveness of the proposed method.
On the High-Temperature Oxidation and Area Specific Resistance of New Commercial Ferritic Stainless Steels
Authors: Bongiorno Valeria , Spotorno Roberto , Paravidino Daniele , Piccardo Paolo
Published: March 2021

Two commercial ferritic stainless steels (FSSs), referred to as Steel A and Steel B, designed for specific high-temperature applications, were tested in static air for 2000 h at 750 °C to evaluate their potential as base materials for interconnects (ICs) in Intermediate Temperature Solid Oxide Fuel Cell stacks (IT-SOFCs). Their oxidation behavior was studied through weight gain and Area Specific Resistance (ASR) measurements. Additionally, the oxide scales developed on their surfaces were characterized by X-ray Diffraction (XRD), Micro-Raman Spectroscopy (μ-RS), Scanning Electron Microscopy, and Energy Dispersive X-ray Fluorescence Spectroscopy (SEM-EDS). The evolution of oxide composition, structure, and electrical conductivity in response to aging was determined. Comparing the results with those on AISI 441 FSS, steels A and B showed a comparable weight gain but higher ASR values that are required by the application. According to the authors, Steel A and B compositions need an adjustment (i.e., a plain substitution of the elements which form insulant oxides or a marginal modification in their content) to form a thermally grown oxide (TGO) with the acceptable ASR level.
Performance Assessment of Magnesium Anodes Manufactured by Sintering Process
Authors: Sanmiguel-May Judith A. , López-Alcantara Ruth , Juárez-Arellano Erick A. , Pérez-Quiroz José T. , Contreras Antonio , Pérez-López Tezozomoc
Published: March 2021

This work shows the performance of cathodic protection systems formed by an API (American Petroleum Institute) X52 steel exposed to seawater and coupled with galvanic anodes of Mg, Mg-1Cr, and Mg-1Nb fabricate by sintering technique at a temperature of 500 °C. Potential monitoring indicates that X52 steel of the three systems remained in the protection zone. Mg-Nb/X52 system showed the more stable potentials since the first day; the recorded values remained between −1.0 and −1.1 V vs. SCE (saturated calomel electrode) during the seven days of exposure time. Current density records show that Mg/X52 system had the most stable values, while the other two systems (Mg-Cr/X52 Mg-Nb/X52,) had current fluctuations. The Mg-X52 system recorded the most negative potential values, which can be attributed to a greater magnitude and a better distribution of the cathodic protection current. However, the Mg-Nb/X52 system had a better result because the current drained by the system was constant throughout the experiment.
Quantitative Assessment of Occipital Metabolic and Energetic Changes in Parkinson’s Patients, Using In Vivo 31P MRS-Based Metabolic Imaging at 7T
Authors: Zhu Xiao-Hong , Lee Byeong-Yeul , Tuite Paul , Coles Lisa , Sathe Abhishek G. , Chen Chi , Cloyd Jim , Low Walter C. , Steer Clifford J. , Chen Wei
Published: March 2021

Abnormal energy metabolism associated with mitochondrial dysfunction is thought to be a major contributor to the progression of neurodegenerative diseases such as Parkinson’s disease (PD). Recent advancements in the field of magnetic resonance (MR) based metabolic imaging provide state-of-the-art technologies for non-invasively probing cerebral energy metabolism under various brain conditions. In this proof-of-principle clinical study, we employed quantitative 31P MR spectroscopy (MRS) imaging techniques to determine a constellation of metabolic and bioenergetic parameters, including cerebral adenosine triphosphate (ATP) and other phosphorous metabolite concentrations, intracellular pH and nicotinamide adenine dinucleotide (NAD) redox ratio, and ATP production rates in the occipital lobe of cognitive-normal PD patients, and then we compared them with age-sex matched healthy controls. Small but statistically significant differences in intracellular pH, NAD and ATP contents and ATPase enzyme activity between the two groups were detected, suggesting that subtle defects in energy metabolism and mitochondrial function are quantifiable before regional neurological deficits or pathogenesis begin to occur in these patients. Pilot data aiming to evaluate the bioenergetic effect of mitochondrial-protective bile acid, ursodeoxycholic acid (UDCA) were also obtained. These results collectively demonstrated that in vivo 31P MRS-based neuroimaging can non-invasively and quantitatively assess key metabolic-energetic metrics in the human brain. This provides an exciting opportunity to better understand neurodegenerative diseases, their progression and response to treatment.
Reconfigurable Metasurface Antenna Based on the Liquid Metal for Flexible Scattering Fields Manipulation
Authors: Qian Ting
Published: March 2021

In this paper, we propose a reconfigurable metasurface antenna for flexible scattering field manipulation using liquid metal. Since the Eutectic gallium indium (EGaIn) liquid metal has a melting temperature around the general room temperature (about 30 °C), the structure based on the liquid metal can be easily reconstructed under the temperature control. We have designed an element cavity structure to contain liquid metal for its flexible shape-reconstruction. By melting and rotating the element structure, the shape of liquid metal can be altered, resulting in the distinct reflective phase responses. By arranging different metal structure distribution, we show that the scattering fields generated by the surface have diverse versions including single-beam, dual-beam, and so on. The experimental results have good consistency with the simulation design, which demonstrated our works. The presented reconfigurable scheme may promote more interest in various antenna designs on 5G and intelligent applications.
Multi-Field Coupling Dynamics Modeling of Aerostatic Spindle
Authors: Chen Guoda , Chen Yijie
Published: March 2021

The aerostatic spindle in the ultra-precision machine tool shows the complex multi-field coupling dynamics behavior under working condition. The numerical investigation helps to better understand the dynamic characteristics of the aerostatic spindle and improve its structure and performance with low cost. A multi-field coupling 5-DOF dynamics model for the aerostatic spindle is proposed in this paper, which considers the interaction between the air film, spindle shaft and the motor. The restoring force method is employed to deal with the times varying air film force, the transient Reynolds equation of the aerostatic journal bearing and the aerostatic thrust bearing is solved using ADI method and Thomas method. The transient air film pressure of aerostatic bearings is obtained which clearly presents the influence induced by the tilt motion of the spindle shaft. The motion trajectory of the spindle shaft is obtained which shows different stability of the shaft under different external forces. The dynamics model shows good performance on simulating the multi-field coupling behavior of the aerostatic spindle under external force. which is quite meaningful and useful for the further research on the dynamic characteristics of the aerostatic spindle.
Alteration of Bentonite Reacted with Cementitious Materials for 5 and 10 years in the Mont Terri Rock Laboratory (CI Experiment)
Authors: Yokoyama Shingo , Shimbashi Misato , Minato Daisuke , Watanabe Yasutaka , Jenni Andreas , Mäder Urs
Published: March 2021

The cement–clay interaction (CI) experiment was carried out at the Mont Terri rock laboratory to complement the current knowledge on the influence that cementitious materials have on Opalinus Clay (OPA) and bentonite (MX). Drill cores including the interface of OPA, concrete (LAC = low-alkali binder, and OPC = ordinary Portland cement), and MX, which interacted for 4.9 and 10 years, were successfully retrieved after drilling, and detailed analyses were performed to evaluate potential mineralogical changes. The saturated compacted bentonites in core samples were divided into ten slices, profiling bentonite in the direction towards the interface, to evaluate the extent and spatial variation of the mineralogical alteration of bentonite. Regarding the mineral compositions of bentonite, cristobalite was dissolved within a range of 10 mm from the interface in both LAC-MX and OPC-MX, while calcite precipitated near the interface for OPC-MX. In LAC-MX and OPC-MX, secondary products containing Mg (e.g., M-S-H) also precipitated within 20 mm of the interface. These alterations of bentonite developed during the first 4.9 years, with very limited progress observed for the subsequent 5 years. Detectable changes in the mineralogical nature of montmorillonite (i.e., the formation of illite or beidellite, increase in layer charge) did not occur during the 10 years of interaction.
Mineralogical Tracers of Gold and Rare-Metal Mineralization in Eastern Kazakhstan
Authors: D’yachkov Boris A. , Bissatova Ainel Y. , Mizernaya Marina A. , Khromykh Sergey V. , Oitseva Tatiana A. , Kuzmina Oxana N. , Zimanovskaya Natalya A. , Aitbayeva Saltanat S.
Published: March 2021

Replenishment of mineral resources, especially gold and rare metals, is critical for progress in the mining and metallurgical industry of Eastern Kazakhstan. To substantiate the scientific background for mineral exploration, we study microinclusions in minerals from gold and rare-metal fields, as well as trace-element patterns in ores and their hosts that may mark gold and rare-metal mineralization. The revealed compositions of gold-bearing sulfide ores and a number of typical minerals (magnetite, goethite, arsenopyrite, antimonite, gold and silver) and elements (Fe, Mn, Cu, Pb, Zn, As, and Sb) can serve as exploration guides. The analyzed samples contain rare micrometer lead (alamosite, kentrolite, melanotekite, cotunnite) and nickel (bunsenite, trevorite, gersdorffite) phases and accessory cassiterite, wolframite, scheelite, and microlite. The ores bear native gold (with Ag and Pt impurities) amenable to concentration by gravity and flotation methods. Multistage rare-metal pegmatite mineralization can be predicted from the presence of mineral assemblages including cleavelandite, muscovite, lepidolite, spodumene, pollucite, tantalite, microlite, etc. and such elements as Ta, Nb, Be, Li, Cs, and Sn. Pegmatite veins bear diverse Ta minerals (columbite, tantalite-columbite, manganotantalite, ixiolite, and microlite) that accumulated rare metals late during the evolution of the pegmatite magmatic system. The discovered mineralogical and geochemical criteria are useful for exploration purposes.
Technologically Sustainable Route for Metals Valorization from Jarosite-PbAg Sludge
Authors: Kamberović Željko , Gajić Nataša , Korać Marija , Jevtić Sanja , Sokić Miroslav , Stojanović Jovica
Published: March 2021

By-products from zinc hydrometallurgy are classified as hazardous waste with strong leaching toxicities. Even though numerous research papers are dedicated to valorizing valuable metals in it, the primary management route is still disposal or partial reuse, such as the Waelz process. Presented experimental research investigates possibilities of sulfidization and further processing as a technologically sustainable route for valuable metals valorization from non-standard jarosite-PbAg sludge. The comprehensive thermodynamic analysis was done by HSC Chemistry®, through optimizing process parameters, i.e., temperature, sulfur addition, and selection of possible additives. Technological possibility of magnetic separation, flotation, and smelting of sulfidized material was also investigated; the results were below the values that allow practical application, due to the obtained texture of sulfidized jarosite, which does not allow the liberation of minerals. Smelting tests were performed on sulfidized jarosite with sulfur and without and with carbon as additive. By smelting sulfidized jarosite-PbAg sludge with added carbon in sulfidization stage at 1375 °C, obtained products were matte, slag, raw lead, and dust in which base, critical, and slag forming components were valorized. Valuable metals were concentrated in smelting products so as to enable further processing, which also could be interesting in the case of treatment of complex, polymetallic, and refractory primary materials, which represent a significant contribution to the circular economy.
Colloidal Stability and Cytotoxicity of Polydopamine-Conjugated Gold Nanorods against Prostate Cancer Cell Lines
Authors: Mahmoud Nouf N. , Aqabani Hakam , Hikmat Suhair , Abu-Dahab Rana
Published: March 2021

Prostate cancer is one of the most common cancers in men. Cell invasion is an important step in the process of cancer metastasis. Herein, gold nanorods (GNRs) and polyethylene glycol (PEG)-coated GNRs were conjugated with polydopamine (PDA). The PDA-nanoconjugates demonstrated excellent colloidal stability upon lyophilization and dispersion in cell culture media with or without the addition of fetal bovine albumin (FBS), compared to unconjugated GNRs. PDA-nanoconjugates exhibited a considerable cytotoxicity against DU-145 and PC3 prostate cancer cell lines over a concentration range of 48 μg/mL–12 μg/mL, while they were biocompatible over a concentration range of 3.0 μg/mL–0.185 μg/mL. Furthermore, PDA-nanoconjugates demonstrated possible anti-invasion activity towards prostate cancer cell lines, particularly DU-145 cell line, by reducing cell migration and cell adhesion properties. The PDA-nanoconjugates could be considered a promising nano-platform toward cancer treatment by reducing the invasion activity; it could also be considered a drug delivery system for chemotherapeutic agents.
Salt and Aroma Compound Distributions Influence Flavour Release and Temporal Perception While Eating Hot-Served Flans
Authors: Emorine Marion , Septier Chantal , Martin Christophe , Cordelle Sylvie , Sémon Etienne , Thomas-Danguin Thierry , Salles Christian
Published: March 2021

To counteract the negative effect of salt overconsumption on health, strategies have been developed to reduce the salt content in food products. Among them, two promising strategies based on odour-induced saltiness enhancement and the heterogeneous distribution of flavour compounds were combined and assessed in four-layer cream-based snacks. To investigate the relationship between saltiness enhancement, temporal release and perception of flavour compounds in hot snacks with heterogeneous distribution of salt and aroma compounds, complementary techniques were used: nose space PTR-Tof-MS (Proton Transfer Reaction-Time of Flight–Mass Spectrometry) to assess the release of aroma compounds in vivo, and ATI (Alternate Time-Intensity) and TDS Temporal Dominance of Sensations) to evaluate perception as a function of time. The obtained results confirmed that the strategy of concentrating salt in the outer layer of a multilayer product was the optimal solution with respect to taste intensity. Heterogeneous salt distribution decreased aroma compound release and consequently aroma intensity but in different ways according to both salt and added aroma distribution in the food matrix. The salty taste enhancement could be due to the initial strong dominance of the salty sensation at the very beginning of the eating process. The involved mechanisms rely on a combination of physico-chemical and perceptual effects which are not clear yet.
Interactions of Sea Anemone Toxins with Insect Sodium Channel—Insights from Electrophysiology and Molecular Docking Studies
Authors: Niklas Beata , Jankowska Milena , Gordon Dalia , Béress László , Stankiewicz Maria , Nowak Wieslaw
Published: March 2021

Animal venoms are considered as a promising source of new drugs. Sea anemones release polypeptides that affect electrical activity of neurons of their prey. Voltage dependent sodium (Nav) channels are the common targets of Av1, Av2, and Av3 toxins from Anemonia viridis and CgNa from Condylactis gigantea. The toxins bind to the extracellular side of a channel and slow its fast inactivation, but molecular details of the binding modes are not known. Electrophysiological measurements on Periplaneta americana neuronal preparation revealed differences in potency of these toxins to increase nerve activity. Av1 and CgNa exhibit the strongest effects, while Av2 the weakest effect. Extensive molecular docking using a modern SMINA computer method revealed only partial overlap among the sets of toxins’ and channel’s amino acid residues responsible for the selectivity and binding modes. Docking positions support earlier supposition that the higher neuronal activity observed in electrophysiology should be attributed to hampering the fast inactivation gate by interactions of an anemone toxin with the voltage driven S4 helix from domain IV of cockroach Nav channel (NavPaS). Our modelling provides new data linking activity of toxins with their mode of binding in site 3 of NavPaS channel.
On the Proton-Bound Noble Gas Dimers (Ng-H-Ng)+ and (Ng-H-Ng’)+ (Ng, Ng’ = He-Xe): Relationships between Structure, Stability, and Bonding Character
Authors: Borocci Stefano , Grandinetti Felice , Sanna Nico
Published: March 2021

The structure, stability, and bonding character of fifteen (Ng-H-Ng)+ and (Ng-H-Ng’)+ (Ng, Ng’ = He-Xe) compounds were explored by theoretical calculations performed at the coupled cluster level of theory. The nature of the stabilizing interactions was, in particular, assayed using a method recently proposed by the authors to classify the chemical bonds involving the noble-gas atoms. The bond distances and dissociation energies of the investigated ions fall in rather large intervals, and follow regular periodic trends, clearly referable to the difference between the proton affinity (PA) of the various Ng and Ng’. These variations are nicely correlated with the bonding situation of the (Ng-H-Ng)+ and (Ng-H-Ng’)+. The Ng-H and Ng’-H contacts range, in fact, between strong covalent bonds to weak, non-covalent interactions, and their regular variability clearly illustrates the peculiar capability of the noble gases to undergo interactions covering the entire spectrum of the chemical bond.
Suppressing Cdk5 Activity by Luteolin Inhibits MPP+-Induced Apoptotic of Neuroblastoma through Erk/Drp1 and Fak/Akt/GSK3β Pathways
Authors: Reudhabibadh Ratchaneekorn , Binlateh Thunwa , Chonpathompikunlert Pennapa , Nonpanya Nongyao , Prommeenate Peerada , Chanvorachote Pithi , Hutamekalin Pilaiwanwadee
Published: March 2021

Parkinson’s disease (PD) is characterized by the progressive degeneration of dopaminergic neurons. The cause of PD is still unclear. Oxidative stress and mitochondrial dysfunction have been linked to the development of PD. Luteolin, a non-toxic flavonoid, has become interested in an alternative medicine, according to its effects on anti-oxidative stress and anti-apoptosis, although the underlying mechanism of luteolin on PD has not been fully elucidated. This study aims to investigate whether luteolin prevents neurotoxicity induction by 1-methyl-4-phenylpyridinium iodide (MPP+), a neurotoxin in neuroblastoma SH-SY5Y cells. The results reveal that luteolin significantly improved cell viability and reduced apoptosis in MPP+-treated cells. Increasing lipid peroxidation and superoxide anion (O2−), including mitochondrial membrane potential (Δψm) disruption, is ameliorated by luteolin treatment. In addition, luteolin attenuated MPP+-induced neurite damage via GAP43 and synapsin-1. Furthermore, Cdk5 is found to be overactivated and correlated with elevation of cleaved caspase-3 activity in MPP+-exposed cells, while phosphorylation of Erk1/2, Drp1, Fak, Akt and GSK3β are inhibited. In contrast, luteolin attenuated Cdk5 overactivation and supported phosphorylated level of Erk1/2, Drp1, Fak, Akt and GSK3β with reducing in cleaved caspase-3 activity. Results indicate that luteolin exerts neuroprotective effects via Cdk5-mediated Erk1/2/Drp1 and Fak/Akt/GSK3β pathways, possibly representing a potential preventive agent for neuronal disorder.
Inhibitory Effect of Elaeagnus umbellata Fractions on Melanogenesis in α-MSH-Stimulated B16-F10 Melanoma Cells
Authors: Lee Ji-Hyun , Lee Bori , Jeon Yong-Deok , Song Hyun-Woo , Lee Young-Mi , Song Bong-Joon , Kim Dae-Ki
Published: March 2021

When skin is exposed to UV radiation, melanocytes produce melanin. Excessive melanin production leads to skin pigmentation, which causes various cosmetic and health problems. Therefore, the development of safe, natural therapeutics that inhibit the production of melanin is necessary. Elaeagnus umbellata (EU) has long been widely used as a folk medicinal plant because of pharmacological properties that include anti-ulcer, antioxidant, and anti-inflammatory properties. In this study, we investigated the antioxidant activity and melanogenesis inhibitory effects of EU fractions in B16-F10 melanoma cells. EU fractions showed a dose-dependent increase in antioxidant activity in radical scavenging activity. In addition, we evaluated the effect of EU fractions on tyrosinase activity and melanogenesis in α-melanocyte-stimulating hormone-induced B16-F10 melanoma cells. EU was noncytotoxic at 12.5–50 μg/mL. EU fractions effectively inhibited tyrosinase activity and melanogenesis, suppressed the phosphorylation of CREB and ERK involved in the melanogenesis pathway, and down-regulated expression of melanogenesis-related proteins. Interestingly, the anti-melanogenesis effect was most effective at a concentration of 50 μg/mL EU, and the effects of the fractions were superior to those of the extract. Therefore, our study suggests that EU has potential as a safe treatment for excessive pigmentation or as a natural ingredient in cosmetics.
The Localization Behavior of Different CNTs in PC/SAN Blends Containing a Reactive Component
Authors: Gültner Marén , Boldt Regine , Formanek Petr , Fischer Dieter , Simon Frank , Pötschke Petra
Published: March 2021

Co-continuous blend systems of polycarbonate (PC), poly(styrene-co-acrylonitrile) (SAN), commercial non-functionalized multi-walled carbon nanotubes (MWCNTs) or various types of commercial and laboratory functionalized single-walled carbon nanotubes (SWCNTs), and a reactive component (RC, N-phenylmaleimide styrene maleic anhydride copolymer) were melt compounded in one step in a microcompounder. The blend system is immiscible, while the RC is miscible with SAN and contains maleic anhydride groups that have the potential to reactively couple with functional groups on the surface of the nanotubes. The influence of the RC on the localization of MWCNTs and SWCNTs (0.5 wt.%) was investigated by transmission electron microscopy (TEM) and energy-filtered TEM. In PC/SAN blends without RC, MWCNTs are localized in the PC component. In contrast, in PC/SAN-RC, the MWCNTs localize in the SAN-RC component, depending on the RC concentration. By adjusting the MWCNT/RC ratio, the localization of the MWCNTs can be tuned. The SWCNTs behave differently compared to the MWCNTs in PC/SAN-RC blends and their localization occurs either only in the PC or in both blend components, depending on the type of the SWCNTs. CNT defect concentration and surface functionalities seem to be responsible for the localization differences.
Vibrational Spectroscopic Analyses and Imaging of the Early Middle Ages Hemp Bast Fibres Recovered from Lake Sediments
Authors: Kalisz Grzegorz , Gieroba Barbara , Chrobak Olga , Suchora Magdalena , Starosta Agata L. , Sroka-Bartnicka Anna
Published: March 2021

Fourier Transform Infrared (FT-IR) spectroscopy and imaging combined with hierarchical cluster analysis (HCA) was applied to analyse biochemical properties of Early Middle Ages hemp (Cannabis sativa L.) bast fibres collected from lake bottom sediment of lake Słone. The examined plant macrofossil material constitutes residues of the hemp retting process that took place in the 7th–8th century. By comparison of three samples: untreated isolated bast fibres, and fibres incubated overnight at 4 and 37 °C, we were able to mimic the retting conditions. Using FT-IR qualitative and semi-quantitative assessment of the primary polysaccharides content, total protein content, and their spatial distribution was performed within the hemp fibres. The concentration of cellulose remained vastly unchanged, while the concentration of lignin and pectin was the highest in the untreated sample. The spatial distributions of compounds were heterogeneous in the untreated and 4 °C-incubated samples, and homogenous in the specimen processed at 37 °C. Interestingly, a higher amide content was detected in the latter sample indicating the highest degree of enzymatic degradation. In this study, we show that the spectroscopic methods allow for a non-destructive evaluation of biochemical composition of plant fibres without preparation, which can be an appropriate approach for studying ancient plant remains.
Additive-Enhanced Exfoliation for High-Yield 2D Materials Production
Authors: Nguyen Dinh-Tuan , Ting Hsiang-An , Su Yen-Hsun , Hofmann Mario , Hsieh Ya-Ping
Published: March 2021

The success of van-der-Waals electronics, which combine large-scale-deposition capabilities with high device performance, relies on the efficient production of suitable 2D materials. Shear exfoliation of 2D materials’ flakes from bulk sources can generate 2D materials with low amounts of defects, but the production yield has been limited below industry requirements. Here, we introduce additive-assisted exfoliation (AAE) as an approach to significantly increase the efficiency of shear exfoliation and produce an exfoliation yield of 30%. By introducing micrometer-sized particles that do not exfoliate, the gap between rotor and stator was dynamically reduced to increase the achievable shear rate. This enhancement was applied to WS2 and MoS2 production, which represent two of the most promising 2D transition-metal dichalcogenides. Spectroscopic characterization and cascade centrifugation reveal a consistent and significant increase in 2D material concentrations across all thickness ranges. Thus, the produced WS2 films exhibit high thickness uniformity in the nanometer-scale and can open up new routes for 2D materials production towards future applications.
Multifunctional Conductive Paths Obtained by Laser Processing of Non-Conductive Carbon Nanotube/Polypropylene Composites
Authors: Cesano Federico , Uddin Mohammed Jasim , Damin Alessandro , Scarano Domenica
Published: March 2021

Functional materials are promising candidates for application in structural health monitoring/self-healing composites, wearable systems (smart textiles), robotics, and next-generation electronics. Any improvement in these topics would be of great relevance to industry, environment, and global needs for energy sustainability. Taking into consideration all these aspects, low-cost fabrication of electrical functionalities on the outer surface of carbon-nanotube/polypropylene composites is presented in this paper. Electrical-responsive regions and conductive tracks, made of an accumulation layer of carbon nanotubes without the use of metals, have been obtained by the laser irradiation process, leading to confined polymer melting/vaporization with consequent local increase of the nanotube concentration over the electrical percolation threshold. Interestingly, by combining different investigation methods, including thermogravimetric analyses (TGA), X-ray diffraction (XRD) measurements, scanning electron and atomic force microscopies (SEM, AFM), and Raman spectroscopy, the electrical properties of multi-walled carbon nanotube/polypropylene (MWCNT/PP) composites have been elucidated to unfold their potentials under static and dynamic conditions. More interestingly, prototypes made of simple components and electronic circuits (resistor, touch-sensitive devices), where conventional components have been substituted by the carbon nanotube networks, are shown. The results contribute to enabling the direct integration of carbon conductive paths in conventional electronics and next-generation platforms for low-power electronics, sensors, and devices.