bluuebunny/crossref_metadata_2025 huggingface dataset:
Efficacy of adding systemic glucocorticosteroids to standard therapy in adolescents with severe acne: a randomized controlled trial
Authors: Kruglova L. S. , Gryazeva N. V. , Tamrazova A. V.
Published: April 2022

Introduction. Isotretinoin is the first-choice drug in the treatment of severe forms of acne vulgaris. The combination of systemic retinoids with durant corticosteroids helps to reduce the likelihood of developing “retinoic” dermatitis and exacerbation of acne in the initial stages of isotretinoin therapy.

Purpose of the study. Determination of the effectiveness of the combined use of isotretinoin and a durant corticosteroid in the treatment of severe forms of acne vulgaris.

Materials and methods. Twenty six patients with “severe” or “very severe” grade on the IGA scale were included in this randomised, controlled comparative study. Thirteen patients (group A) were treated with isotretinoin 0.5 mg/kg/day (cumulative dose from 120 to 150 mg/kg) for 8 months and 2 injections of betamethasone dipropionate + betamethasone sodium phosphate at dose 1 ml (2 mg + 5 mg/1 ml) at first month (1 injection per two weeks) and thirteen patients (group B) were treated with combined therapy with isotretinoin at a dose of 0.5 mg/kg/day (cumulative dose from 120 to 150 mg/kg) for 8 months and assessment was based on the IGA scale, counting the number of inflammatory and non-inflammatory elements, indicators DIA (dermatological index of acne) and DLQI and was done at baseline, 1, 4 and 8 months of treatment.

Results. At month 8, compared to group B, group A showed more significant decrease in IGA score and 76% patients achieved “clear” or “almost clear skin” degree (76% vs. 30%). The reduction in the number of inflammatory and non-inflammatory elements showed a marked clinical improvement in group A (89.2% vs 22.3 % for nodules). The decrease in DIA was 88.3% in group A and 71.3% in group B. Exacerbations of acne were recorded in 0% (group A) vs 38.0% (group B) of patients. We also found a relationship between the achievement of a 2-point reduction in the degree on the IGA scale after 8 months and the presence of exacerbations while taking isotretinoin (p = 0.012). Analyzing the DLQI between the two, we were unable to identify statistically significant differences.

Conclusions. Combines use of long acting steroid with isotretinoin provides synergic effect while minimizing the side effect of isotretinoin (decreases the number of exacerbations), demonstrates a visible effect to patients within a month, thereby increasing compliance, improving the quality of life and reducing the risk of scarring.
A modern view on the treatment of psoriatic disease. Experience in using genetically engineered biological therapy in patients with extensive moderate to severe psoriasis
Authors: Svechnikova E. V. , Zhufina S. E.
Published: April 2022

The article presents a modern view on the pathogenesis of psoriasis, considers an approach to management of patients with extensive moderate to severe psoriasis, sets out the key features of treatment with genetically engineered biological drugs of different groups: TNF-alpha, IL-17, IL-23 inhibitors. Literature data on the key features, indications, contraindications, and side effects associated with the use of genetically engineered drugs have also been analysed. The experience of using therapies described in domestic and foreign research publications was examined. Approaches to the treatment of patients with moderate to severe psoriasis combined with concomitant comorbid pathologies were discussed, and the features of treatment with genetically engineered biological drugs of different groups were also evaluated. In addition, the publication contains the results of our own observations obtained in the treatment of patients with extensive psoriasis and concomitant comorbid pathology using such biological drugs as secukinumab (10 patients), netakimab (5 patients), guselcumab (7 patients). The schemes of patient investigation, dosage regimens for each drug are given, their efficacy and tolerability are evaluated, and complications acquired during treatment with each drug are analysed, the speed and stability of the therapeutic effect provided by each of them are evaluated. In connection with the emergence of new data on the pathogenesis and treatment of psoriasis, patients have increasing opportunities to receive timely care, maintain disease remission for a long time and improve the quality of life regardless of the severity and stage of the pathological process, as well as of the history and presence of comorbid conditions.
Changes in cornea structure after corneal collagen crosslinking in keratoconus
Authors: Avetisov S. E. , Bubnova I. A. , Surnina Z. V. , Averich V. V. , Sarkisova K. G.
Published: April 2022

Introduction. The article considers an objective assessment of the state of morphofunctional status of cornea in keratoconus after a corneal collagen crosslinking procedure.

Aim. To assess changes in cornea structure after corneal collagen crosslinking in keratoconus. Materials and methods. The study included 24 patients: 30 eyes with KC stage I–III aged 17 to 42 years. The patients were examined before and after the  corneal collagen crosslinking procedure. The postoperative follow-up period was 12  months. The patients underwent anterior segment OCT (AS-OCT) imaging to assess the demarcation line depth. The cornea and cornea nerve fibers were assessed layer-by-layer using сonfocal laser scanning microscopy, followed by the  analysis of  resulting confocal images through the author’s analysis algorithm.

Results and discussion. The epithelialization of the cornea completed on day 3–5 after the procedure. According to OCT findings, the depth of the demarcation line averaged to 260 µm in the center and 140 µm in the periphery. The pronounced edema of the outer stroma was observed during the first-week follow-up, and a decrease in the density and apoptosis of keratocytes was noted during the first month. Over a 3–12-month postoperative follow-up period, the transient lacunar edema regressed and the density of keratocytes was restored to the baseline level. During the first three months, a pronounced disruption of the direction and structure of the cornea nerve fibres is seen.

Conclusion. The crosslinking procedure results in changes in the cornea structure, one of which is appearance of the demarcation line in the stroma, which indicates the depth of penetration of the photochemical corneal collagen crosslinking process. The laser corneal confocal microscopy allows to objectively assess the depth of this effect, while the values obtained in the same follow-up periods are comparable with the findings of OCT imaging.
New opportunities in the treatment of asthenic symptoms after a new coronavirus infection
Authors: Shishkova V. N. , Martynov A. I.
Published: April 2022

Introduction. Asthenia is an urgent problem during the pandemic of new coronavirus infection (COVID-19) because of its high frequency regardless of the severity of the disease.

The purpose of this subanalysis of data from the multicenter controlled randomized clinical trial TONUS was to evaluate the efficacy and safety of meldonium therapy for аsthenia in COVID-19 survivors.

Materials and methods. A total of 880 patients with asthenia who underwent COVID-19 within the last 6 months were included in the analysis. The efficacy of asthenia therapy was assessed by the MFI-20 scale, Schulte tables, and the General Clinical Impression (CGI) scale. All patients were previously randomized in two parallel branches of the TONUS study, including patients without concomitant disease in TONUS-1 (who received meldonium 500 mg/day for 14 days in the main group) and patients with cardiovascular or cerebrovascular disease in TONUS-2, who received meldonium at a dose of 1000 mg/day for 42 days in the main group. In both arms of the study, the drugs in the comparison groups were multivitamin complexes.

Results. For the TONUS-1 groups.In the group of patients receiving meldonium compared with the control group (p < 0.001): total MFI-20 score decreased and was 31 (25; 40); MFI-20 –35 (–46; 23); performance value –5 (–11; –2) seconds; proportion of patients with significant improvement (by CGI-I) by the end of follow-up was 92.8%. For TONUS-2 groups.In the group of patients receiving meldonium compared with the control group (p < 0.001): total MFI-20 score decreased to 35 (27; 44); MFI-20 –34 (–46; –21), performance value –5 (–11; –2), proportion of patients with significant improvement (by CGI-I) by end of follow-up – 90.8%.

Conclusion. Significant positive dynamics and regression of asthenia were noted in the groups of patients receiving meldonium in comparison with the control groups.
Scoliotic deformity of the spine: epidemiology, classification, surgical treatment, prediction and correction of intraoperative blood loss
Authors: Smirnov I. V. , Rojtberg G. E. , Lazarev V. V. , Buralkina N. A. , Batyrova Z. K.
Published: April 2022

In this review are discussed the most important questions of diagnostics, surgical treatment and complications in the context of  anaesthesia choice and performance in  children and adolescents with different variants of  vertebral scoliotic deformity. Vertebral scoliotic deformity is a multi-etiological disease and significant clinical problem due to frequency and severity of complications when disease progresses. Surgery is performed in  severe and super severe scoliotic deformity when conservative treatment is not effective. Surgical treatment of severe scoliotic deformity allows to improve physiological function of vertebral column and internal organs’ function, improve quality of life and increase life expectancy. Surgical treatment of severe scoliotic deformity is one of the most complex problem in traumatology and orthopedics which requires multidisciplinary coordination of surgeon, anesthesiologist, narrow specialists and the patient at every step of treatment. The most significant problems in surgical treatment of severe scoliotic deformity are forecasting and blood loss management during the operation and in post-operative care which could reach several circulating blood volumes. The problems of blood loss minimization are discussed: patient’s position on operating table, acute normovolemic haemodilution, managed hypotension, use of  antifibrinolythic medications, blood collection and re-infusion, blood transfusion. Blood loss forecasting is an important instrument to get a proactive information to develop personalized approach to patient’s care with assessment of intraoperative blood loss, risk of hemorrhagic shock and disseminated intravascular coagulation syndrome.
Streptococcal toxic shock syndrome in an adolescent
Authors: Ryzhakova N. A. , Krivonogova T. S. , Golikova E. V. , Zhelev V. A. , Loshkova E. V. , Terentyeva A. A. , Solnyshko A. L. , Vaganova T. V. , Liulka T. S.
Published: April 2022

A  special form of  streptococcal infection is streptococcal toxic shock syndrome  (STS), characterized by rapid development of symptoms and high mortality. Patient O., 14 years old, was taken to the infectious diseases department of OGAUZ DBNo. 1 by the SMP team with complaints of shortness of breath, vomiting, loose stools in a state of moderate severity due to intoxication syndrome. Diagnosis upon admission: Acute infectious gastroenteritis of moderate severity. Acute respiratory infections rhinopharyngitis, acute bronchitis, pneumonia  (?), DN1. During examination in  the UAC, anemia, leukocytosis, acceleration of ESR, in the biochemical blood analysis – an increase in CRP, in the coagulogram — increased INR, APTT, RFMC, decreased PTI, in urine tests – protein, erythrocytes, on the X–ray — bilateral pleural effusion, in the tank. sputum culture — Streptoccocus oralis 10/3 KOE/ml, PCR SARS-CoV-2: negative, blood test for antistreptolysin-O (ASL-O): 800 IU/ml (norm up to 200 IU/ml), blood for sterility 19.05.20: no bacterial microflora growth was detected. After receiving laboratory data, the diagnosis was made: Acute glomerulonephritis?, Аcute intestinal infection. Double-sided hydrothorax. Internal combustion engine. Anemia of the 1st degree. The final diagnosis: Acute post-streptococcal glomerulonephritis with a debut in the form of streptococcal toxic shock syndrome, a period of extensive clinical and laboratory changes, with a decrease in the debut of kidney function in the form of acute renal failure, recovery period. Against the background of the treatment (2 courses of antibiotic therapy  (cefotaxime, amoxicillin), infusion therapy, pulse therapy with metipred  (5  pulses), double transfusion of  freshly frozen plasma, prednisone, lasix, veroshpiron, enap, curantil, heparin, and other accompanying therapy), pronounced positive clinical and laboratory dynamics was noted. She was hospitalized for 43 days, of which 9 days were in the intensive care unit (5 days on a ventilator). On the 44th day, the child was discharged in a satisfactory condition with recommendations under the supervision of a pediatrician, a pediatric nephrologist at the place of residence
Fosfomycin in antibiotic prophylaxis of infectious complications after biopsy of the prostate
Authors: Grigoriev N. A. , Abdullin I. I. , Loginov A. V. , Zhilyaev E. V.
Published: April 2022

Introduction. Biopsy of prostate is a routine urologic procedure. More than 1 million biopsies are performed worldwide annually. The frequency of infectious-inflammatory complications remain high, despite the recommended antibiotic prophylaxis schemes.

Aim. The evaluation of  effectiveness and safety of  combined antimicrobial prophylaxis: Fosfomycin and Fluoroquinolones of 3rd generation.

Materials and methods. 80 patients underwent prostate biopsy in our study and were divided into 2 groups: the first group of 40 patients received routine prophylaxis: Levofloxacin 500 mg 6 hours before the biopsy and 500 mg per day during 4 days after biopsy. The second group of 40 patients, along with standard prophylaxis as in the first group, additionally after biopsy received Fosfomycin 3 gr single-shot.

Results and discussion. In the first group, infectious and inflammatory complications occurred in 8 patients (20%). 12.5% of patients from the first group were hospitalized for paranteral antibiotic therapy. The average length of stay in hospital was 3.4 ± 1.45 days. In all cases, in the first group of patients, E. coli was detected, in 70% of cases fluoroquinolone-resistant strain of the bacterium was received. In the second group of patients now hospitalization was required. One patient out of forty (2.5%) from this group showed signs of urinary tract infection, which was not accompanied by an increase of body temperature, as well as changes in blood and urine tests.

Conclusion. Our results show good effectiveness and safety of fosfomicin for antibiotic prophylaxis for transrectal prostate biopsy.
The Role of Heat Shock Protein 27 in Carcinogenesis and Treatment of Colorectal
Cancer
Authors: Asgharzadeh Fereshteh , Moradi-Marjaneh Reyhaneh , Marjaneh Mahdi Moradi
Published: April 2022

The incidence of colorectal cancer (CRC) has significantly increased in recent decades, which has made this disease an important global health issue. Despite many efforts, there is no useful prognostic or diagnostic biomarker for CRC. Heat shock protein 27 (Hsp27) is one of the most studied members of Hsp family. It has attracted particular attention in CRC pathogenesis, since it is involved in fundamental cell functions for cell survival. Evidence show that Hsp27 plays important roles in CRC progression and metastasis. Hsp27 overexpression has been observed in CRC and suggested to be associated with CRC poor prognosis. In the present review, we focus on the current knowledge on the role of Hsp27 in CRC carcinogenesis and the underlying mechanisms. In addition, we discuss the value of targeting Hsp27 in CRC treatment.
A Combined QSAR and Molecular Docking Approach for Identifying Pyrimidine Derivatives as Penicillin Binding Protein Inhibitors
Authors: Sharma Smriti , Sharma Brij K. , Jain Surabhi , Gulyani Puja
Published: April 2022

Background:

Antimicrobial resistance has been rising continuously in the past few years due to the overuse and exploitation of existing antimicrobials. This has motivated the search for a novel scaffold that has the capability of rapid antimicrobial action. The hybridized pyrimidines have attracted us due to their widespread biological activities, such as anti-bacterial and antifungal activities.

Objective:

The present study incorporates a series of pyrimidine-based antimicrobial agents for the 2D quantitative structure-activity relationship analysis (2D QSAR) and docking analysis.

Methods:

The exploration of the chemical structures in combination with the biological activity in CPMLR led to the detection of six descriptors (Constitutional descriptors, Topological descriptors, Modified Burden Eigenvalues and 2D autocorrelations) for modeling the activity. The resulted QSAR model has been validated using combinatorial protocol in multiple linear regression (CP-MLR) and partial least squares (PLS) analysis.

Methods:

The exploration of the chemical structures in combination with the biological activity in CPMLR led to the detection of six descriptors (Constitutional descriptors, Topological descriptors, Modified Burden Eigenvalues and 2D autocorrelations) for modeling the activity. The resulted QSAR model has been validated using a combinatorial protocol in multiple linear regression (CP-MLR) and partial least squares (PLS) analysis.

Results:

The best QSAR model displays the r2 t value of 0.594, Q2 LOO value of 0.779, Q2 L5O value of 0.767. Further docking study was executed using Autodock Vina against Penicillin-binding protein (PBP2a).

Conclusion:

From the results, Compounds 4, 11and 24 were found to possess a good binding affinity towards PBP2a.
Peripheral Vascular Disease is Associated with Impaired Activities of
Daily Living in the Elderly with Dementia: A Prospective Study
Authors: Safer Umut , Naharci Mehmet Ilkin , Tasci Ilker , Safer Vildan Binay
Published: April 2022

Background:

Although distinct disorders, peripheral vascular disease (PVD) and dementia
are both associated with a progressive decline in activities of daily living in elderly patients.

Objective:

This study aimed to compare the functional performance scores between elderly patients
with and without dementia and with or without PVD.

Methods:

Patients with Alzheimer’s disease, vascular dementia, and mixed type dementia and controls
were prospectively enrolled. Functional performance scores for basic activities of daily living (BADL)
and instrumental activities of daily living (IADL) were evaluated using the Barthel scale and Lawton
scale, respectively. PVD was diagnosed using the ankle brachial index (ABI).

Results:

Controls without PVD were age- and sex-matched with 57 patients with both dementia and
PVD and with 69 patients without dementia. The patients with PVD in both groups had lower mean
BALD scores. Adjusting for age, clinical dementia rating, and depression, PVD was associated with a
higher likelihood of being in the quartiles of lower BADL scores in those with dementia (p=0.020).
Adjusting for age, sex, Mini-Mental State Examination (MMSE) score, depression, and comorbidity
and drug counts among the patients without dementia, a significant association was observed with
PVD and a higher likelihood of being in the quartiles of lower BADL scores (p=0.044). PVD was
related to a higher likelihood of being in the quartiles of lower IADL scores in the non-dementia subjects
(p=0.001) after adjusting for age, depression, MMSE, education, and comorbidity count.

Conclusion:

PVD presence determined the poorer status of BADL in demented individuals but not of
the level of IADL. It is still unclear whether modifying PVD health risks and undergoing ABI screening
may help demented people become more independent.
A Case Series of Hypertrophic Cardiomyopathy Conducted in Vietnam Revealing a Novel Pathogenic Variant of the TNNT2 Gene
Authors: Pham Hung Manh , Tran Van Khanh , Mai Trung Anh , Luong Long Hoang , Le Pham May , Nguyen Chi Khanh , Nguyen Hoai Thu Thi , Pham Minh Nhat , Thuy Can , Le Thanh Tuan , Van Ta Thanh , Tran Thinh Huy
Published: April 2022

Background:

Hypertrophic Cardiomyopathy (HCM) is one of the leading causes of sudden cardiac death in adults.HCM is inherited in an autosomal dominant manner; however, the genetic etiology of the disease is not fully explained and studies on the hereditary characteristics in family trees are still underway.

Methods:

Ten HCM patients and 31 of their relatives were recruited. Targeted sequencing for 4 HCM related-genes, including MYH7, MYBPC3, TNNT2, and TNNI3, using targeted next-generation sequencing (NGS) was carried out. Demographic, clinical, electrocardiography, and echocardiography characteristics were also characterized.

Results:

Among the 10 HCM patients, 5 were identified with the HCM pathogenic variants in MYH7 (3 patients), MYBPC3 (1 patient), and TNNT2 (1 patient) genes. Eleven out of 31 relatives from these 5 genotype-positive patients carried the same pathogenic variants. We found the novel c.822-2 A>G variant in the splicing site of the TNNT2 gene responsible for HCM disease in a family with 7 subjects genotype positive and 3 others who suffered from sudden cardiac death.

Conclusion:

This case series highlighted the importance of genetic testing for clinically confirmed HCM patients and family members. The genetic information can be used as a molecular marker to complement the clinical presentation in the diagnosis of HCM, as well as a prognostic tool for the patients and their family members.
Withdrawn: IOT Monitoring of Debris Flow for Rigid Barrier
Authors: Ming Cheng Yung
Published: April 2022

Since the authors are not responding to the editor’s requests to fulfill the editorial requirement, therefore, the article has been
withdrawn.

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The Important Nutritional and Wonderful Health Benefits of Cashew
(Anacardium occidentale L.)
Authors: Shahrajabian Mohamad Hesam , Sun Wenli
Published: April 2022

Traditional medicines are considered as both food items and medicine, which have been
used for thousands of years. They contain aerial and underground parts of plants as active ingredients,
and they also contain a variety of pharmacologically active plant constituents. The aim of this
study was to summarize and highlight the most important traditional and modern health benefits of
cashew nuts. The literature search was conducted in PubMed, Science Direct, Scopus, Medline and
Google Scholar databases. The main compounds of cashew nuts are β-phellandrene + limonene,
methyl chavicol, germacrene D, germacrene B, trans-α-bergamotene, β-copaene, linalool, α-cadinol,
β-phellandrene, β-caryophyllene, β-bisabolene, 9-epi-(E)-caryophyllene, α-phellandrene, epi-α-
cadinol, hexadecanoic acid, and epi-α-muurolol. The most important pharmacological benefits of
Anacardium occidentale are anti-bacterial activities, anti-diabetic activities, anti-diarrheal activities,
anti-fungal activities, anti-hyperglycemic activities, anti-inflammatory activities, anti-microbial
activities, anti-oxidant activities, anti-tumor activities, insecticidal activities, and wound healing.
Cashew nut is a high potential natural functional food because of its high pharmacological activities
and it being an efficacious natural drug.
Extraction, Isolation, and Structural Elucidation of Novel Phytoconstituents
from Ficus auriculata Lour
Authors: Sunnapu Prasad , Valiyaparambil Shilpa , Kotakonda Muddukrishnaiah , Yogananthan Dhanapal , Benson Samuel Thavamani
Published: April 2022

Aim:

In the present study, the phytochemical screening was carried out with the methanolic
extract of Ficus auriculata Lour figs.

Background:

Naturally occurring phytoconstituents have long been utilised to treat illnesses, and
these substances have provided natural product chemists with a source of structural diversity. Natural
products are usually cited as a rich source of chemical variations in the search for new pharmacological
leads or compounds.

Objective:

The investigation of chemical constituents of the methanolic extract resulted in the identification
of a novel phytoconstituent, namely, 10-methyl-9,10-dihydroacridin-1-ol (A), with acridine
as a basic nucleus.

Methods:

The structure of the compound was established by spectroscopic analysis using FT-IR,
1HNMR, 13CNMR, and MS.

Results:

The purity of the compound was confirmed by RP-HPLC. Forty-eight known and reported
compounds were also identified, along with novel compound-A. Among the forty-eight known
compounds, forty-five compounds were first reported in Ficus auriculata Lour methanolic extract
and the remaining three compounds (Campesterol, Stigmasterol, and Lanosterol) in the Ficus family.
Based on the previous studies, acridine moieties have a significant role in biological activities.

Conclusion:

The compound-A, 10-methyl-9,10-dihydroacridin-1-ol was successfully isolated from
the methanolic extract of the Ficus auriculata Lour fig. The structure of the novel compound was
established by various spectral data obtained from FT-IR, 1HNMR, 13CNMR, and LC-MS/MS.

Others:

The isolated novel acridine derivative may be helpful for the development of lead moieties
which may have various applications.
Glomerular filtration rate in children with juvenile idiopathic arthritis
Authors: Borysova T.P. , Samsonenko S.V.
Published: April 2022

Today, it is proved that kidney injury in children with juvenile idiopathic arthritis (JIA) is characterized by a subclinical course and most often remains undiagnosed. One of the main methods to diagnose kidney lesions is determination of estimated glomerular filtration rate (eGFR). The aim of the study was to determine the most optimal method for eGFR evaluation in children with JIA by comparing different calculation methods based on serum creatinine (original Schwartz formula, Counahan-Barratt formula), serum cystatin C (cystatin C-based equation and Hoek formula). Materials and methods. Eighty children with JIA were examined. Serum creatinine level was determined twice (first and third month of study) by the colorimetric kinetic Jaffe reaction, serum cystatin C concentration was evaluated once, in the third month of the study by enzyme immunoassay. We used methods of variation statistics. Results. Parameters of eGFR by cystatin C-based equation 2012 compared to the original Schwartz formula and Counahan-Barratt formula amplified the rate of eGFR disorders by 81.3 % (p < 0.001) and 47.3 % (p < 0.001) in the first month of the study and by 81.3 % (p < 0.001) and 55.0 % (p < 0.001) in the third month, respectively. A similar comparison of the results of Hoek formula with Schwartz and Counahan-Barratt formula showed that according to Schwartz formula, a decrease in the GFR was 41.3 % less frequent (p < 0.001) in both the first and third month. Counahan-Barratt formula in the first study of serum creatinine showed a difference in eGFR compared to Hoek formula by 7.5 % (p < 0.05). During the second study of serum creatinine, the frequency of eGFR reduction according to Counahan-Barratt formula was detected by 15.0 % less often than according to Hoek method (p < 0.09). Conclusions. Changes in renal function differ depending on the formula of eGFR evaluation. The most optimal method to determine eGFR in children with JIA is Hoek formula based on serum cystatin C. In case of limited ability to analyze serum cystatin C, Counahan-Barratt formula based on serum creatinine can be used.
Structure of sensitization and clinical course of asthma in school-age children sensitized to cat allergens
Authors: Kryvopustovа M.V.
Published: April 2022

Background. Asthma is an actual problem of modern pediatrics, and allergy to cats is important in its development. The study was aimed to investigate the structure of sensitization and features of the clinical course of asthma in school-age children sensitized to cat allergens. Materials and methods. The results of a study of 430 children aged 6–17 years with sensitization to cat allergens were analyzed. Results. Sensitization to Fel d 1 was detected in 96.05 % of patients, Fel d 2 — in 6.74 %, Fel d 4 — in 21.63 %, Fel d 7 — in 25.81 %. Sensitization to one of the allergens of cats prevailed, among the combinations — a combination of Fel d 1 and Fel d 7. Concomitant sensitization to pollen, dogs, house dust mites, food, etc. is also shown. There is a strong correlation between sensitization to Fel d 7 cat lipocalin and Can f 1 dog lipocalin. The correlation between the presence of severe asthma and the number of cat allergens, sensitization to three or more cat allergens, to three lipocalin Fel d 4, Fel d 7, Can f 1 is shown. Conclusions. In school-age children with asthma and sensitization to cat allergens, sensitization to uteroglobin Fel d 1 dominates. Sensitization to lipocalin Fel d 7 was detected in 25.81 % of cases. There is a direct correlation between the presence of severe asthma and the number of allergens in cats. The cross-reactivity of cat lipocalin Fel d 7 and the basic canine allergen Can f 1 should be considered. The clinical significance in severe asthma sensitization to three lipocalins Fel d 4, Fel d 7, Can f 1 is shown.
Current issues of the research process as a component of the protection of the reproductive potential of adolescent girls
Authors: Dynnik V.O. , Vodolazhskyi M.L. , Sydorenko T.P. , Fomina T.V. , Koshman T.V.
Published: April 2022

Background. The medical community extremely needs to conduct scientific research on topical issues of physiology, pathology of the formation of the reproductive system of the body and the organization of medical and preventive measures to preserve the reproductive potential of young people. The study was aimed to improve scientific and informational support for specialists in the field of child and adolescent health protection in pediatric gynecology. Materials and methods. There were studied the publications of research and dissertation works conducted in Ukraine in 2010–2020 in the field of “Pediatric Gynecology”; scientific journals devoted to topical issues of reproductive health of the female population; statistical materials of the Ministry of Health of Ukraine on the analysis of demographic processes. Information analytics methods were used to analyze them. Results. In Ukraine, research work on topical issues of pediatric gynecology is consistently carried out. During the study period (2010–2020), 23 research projects and 26 dissertations were conducted. According to their implementation, 195 articles, 196 abstracts were published in collections of scientific and practical conferences; 6 book publications, 12 methodological recommendations, 11 informational letters, 39 patents for inventions and utility models, 16 certificates of copyright registration were published. The thematic focus of research is presented as follows: pathophysiological mechanisms of formation of menstrual disorders in adolescent girls; disorders of menstrual function in the age aspect; the influence of socio-economic conditions on the formation of the reproductive system of girls and adolescents; new medical technologies in the field of reproductive health. Conclusions. Further research on the mechanisms of the development of gynecological diseases in adolescent patients in modern society will improve the system of preventive and rehabilitation measures to preserve reproductive potential and improve the quality of life of patients with disorders of the reproductive system.
Review of foreign literature on the problem of children and adolescent obesity and its sequelae
Authors: Bulat L.M. , Lysunets O.V. , Didyk N.V.
Published: April 2022

The purpose is to study modern prevalence of overweight in the children’s population, sequelae of this physical disorder and to analyze new diagnostic biomarkers comorbidity in obesity. The data of professional domestic and foreign literature were processed and generalized. Diagnostic criteria of overweight in childhood introduced by the European, American professional societies and the World Health Organization guidelines have some features and are not identical and unified. Body mass index and percentage of fat tissue are common important clinical criteria. The main pathogenetic links that are activated in overweight children and lead to the long-term consequences are the inflammatory response, mitochondrial and endothelial dysfunction, which is associated with the manifestation of pulmonary comorbidity and impaired cardiovascular well-being. The mortality rate associated with acute coronary syndrome is likely to be higher in patients with very high body mass index than in those with lower weight (7.1 vs. 4.8 ‰). Endothelial dysfunction at the preclinical level of damage to organs and systems determines the severity of the disease in the future, being the cause of comorbidity. Intercellular adhesion molecule 1 and vascular cell adhesion molecule 1 determine the degree of activity of circulating endothelial cells (CEC). There is a positive correlation between CEC activity, increased visceral adipose tissue, and low-density lipoprotein cholesterol, while the amount of CEC is associated with adipose tissue volume and the level of systolic blood pressure. Qualitative and quantitative changes in CEC can be used as diagnostic markers when forming risk groups for further monitoring and control.
Guillain — Barré syndrome following asymptomatic COVID-19 in an adolescent: a clinical case
Authors: Koloskova O.K. , Tkachuk R.V. , Garas M.N. , Bilous T.M. , Romanchuk L.I. , Sorochan D.I.
Published: April 2022

Guillain — Barré syndrome (GBS) is a rare disease that occurs with an approximate incidence of 1.7 cases per 100,000 children in the pediatric population. The gender difference in the prevalence of the disease is characterized by a 20% greater vulnerability of boys than girls. The exact cause of Guillain — Barre syndrome is unknown; however, it can occur after respiratory infection by Haemophilus influenzae, recent or ongoing Campylobacter jejuni infection or poliovirus. Since the beginning of the COVID-19 pandemic, there have been some reported Guillain — Barré diagnoses among adults following SARS-CoV-2 infection. The article describes a clinical case of an adolescent girl who was admitted to the Regional Municipal Non-Profit Facility “Chernivtsi Regional Children’s Clinical Hospital” with signs of flaccid paraplegia of the lower extremities, pelvic dysfunction and paraparesis of the upper extremities after she had suffered a sudden severe pain syndrome. She had previously been diagnosed with asymptomatic SARS-CoV-2. Initial complex investigations were as follows: high white blood cells count, elevated levels of procalcitonin (PCT), C-reactive protein and D-dimer, low SARS-CoV-2 IgM level and positive IgG titer, high levels of protein in spinal fluid. Electroneuromyography findings and nerve conduction test of lower extremities showed decreased M amplitude and H reflex response. The patient received oxygen therapy, hemodynamic support, cardiac support (infusions and inotrope therapy), gradual antibacterial therapy and was administrated with intravenous normal human immunoglobulin. She was also treated with a short course of parenteral glucocorticoids, vasoactive neurometabolites, nootropics, anticholinesterases, non-steroidal anti-inflammatory drugs along with physical therapy and massages. The patient began demonstrating slow but positive improvement in her limb motor functions and sensitivity within one month.
Mechanisms of action of intranuclear microRNAs. Part 2. MicroRNA-mediated regulation of ncRNA transcriptome and alternative splicing
Authors: Abaturov A.E. , Babуch V.L.
Published: April 2022

The scientific review presents the mechanisms of action of intranuclear miRNAs, namely microRNA-mediated regulation of the non-coding RNA (ncRNA) transcriptome and alternative splicing. To write the article, information was searched using Scopus, Web of Science, MedLine, PubMed, Google Scholar, EMBASE, Global Health, The Cochrane Library, CyberLeninka databases. It is emphasized that a significant effect on the composition of the transcriptome is provided by microRNAs in the RNA-induced silencing complex that induce posttranscriptional degradation of long ncRNAs localized in the cell nucleus. Scientists believe that long ncRNAs are involved in the epigenetic regulation of gene silencing through chromatin remodeling. It is shown that long ncRNAs are actively involved in the development of some liver diseases. It is reported that the most important mechanism for expanding the spectrum of the transcriptome in the cell is the alternative splicing of pre-mRNA. The authors present the results of scientific studies that show that pre-mRNA of more than 90 % of human genes are subjected to alternative splicing. It is presented that splicing is performed by a specialized macromolecular formation — suprasplicesome, which is a megacomplex (21 MDA) of nuclear ribonucleroprotein. It is shown that scientists have proposed two models of epigenetic regulation of splicing: kinetic and recruitment one. The authors reveal the main provisions of these models. MicroRNAs are actively involved in splicing. Liver disease may be based on a deficiency of splicing factors and deregulation of alternative splicing caused by the action of miRNAs. Disorders of alternative splicing, which stimulate proliferation, prevent apoptosis and support cell transformation, are a pathognomonic phenomenon in malignant tumors. Thus, the constituent mechanisms of action of intranuclear miRNAs are alteration of the ncRNAs transcriptome and participation in the regulation of alternative splicing. MicroRNA-mediated regulation of the stability of long ncRNAs causes a change in the spectrum of activity of expression of epigenetically regulated genes. Long ncRNAs are actively involved in the development of some liver diseases. Alternative splicing is an integral part of cell differentiation and contributes to the formation of tissue specificity. Alternative splicing and generation of various isoforms of proteins determine molecular consequences that cause the development of various pathological conditions.
Effects of manure and chemical fertilizers on maize performance in the steep hillside with and without terraces in North Halmahera, Indonesia
Authors: Rofita Rofita , Utami Sri Nuryani Hidayah , Maas Azwar , Nurudin Makruf
Published: April 2022

Improper soil management inland with steep slopes is one of the causes of decreased agricultural productivity in hilly areas. The use of terraces on steep slopes is considered effective in preventing erosion. However, this does not increase agricultural productivity significantly. Therefore, this study aimed to analyze the performance of maize on land with and without terraces as affected by the addition of goat manure and NPK. A field experiment was arranged in a randomized complete block design, consisting of 12 experimental plots that were made on a hill with a slope of 79 % with three replications. Plant tissue samples were analyzed for assessing N, P, and K content. However, soil samples were measured for N, P, K, SOC, pH H2O, EC, CEC, and soil texture. Data were analyzed using analysis of variance and continued with a Post-Hoc test (DMRT at α= 5%). The application of manure and NPK fertilizers significantly improved EC, SOC, N, and P in soil, also N and P content in plant tissues. Goat manure played a role in improving soil properties, while NPK fertilizers played a role in supplying nutrient availability directly to plants. The best performance of maize was on land without terraces and cover crops (sweet potato). Competition for nutrients between maize and sweet potato occurred on land with terraces so that the performance of maize tended to be lower. Soil management on land with terraces needs to consider nutrient management and selection of cover crops so that both can increase maize productivity.
Potensi Senyawa Nitazoxanide dan Arbidol sebagai Antivirus SARS-CoV-2 terhadap Reseptor NSP5 (7BQY dan 2GZ7) dan ACE2 (3D0G dan 1R4L)
Authors: Ami Fini Faqiha  , Indrawijaya Yen Yen Ari , Suryadinata Arief , Amiruddin Muhammad , Mutiah Roihatul
Published: April 2022

Nitazoxanide merupakan obat pro-drug untuk tizoxanide. Obat tersebut diketahui memiliki aktivitas antivirus pada MERS-CoV. Arbidol memiliki aktivitas antivirus pada H3N2 HA yang memiliki kesamaan struktur dengan SARS-CoV-2. Reseptor target spesifik untuk obat-obat Anti SARS-CoV-2 saat ini adalah NSP5 (Nonstructural protein) dan ACE2 (Angiotensin-converting enzyme 2). NSP5 memediasi replikasi dan transkripsi protein-protein virus sedangkan ACE2 sebagai fasilitator transport asam amino dan reseptor tempat terikatnya SARS-CoV maupun SARS-CoV-2. Tujuan penelitian ini adalah untuk mengetahui aktivitas antivirus SARS-CoV-2 dari senyawa Nitazoxanide dan Arbidol secara in silico. Senyawa Nitazoxanide dan Arbidol dipreparasi terlebih dahulu dengan melakukan pengunduhan kode PDB ID, penyiapan struktur 2D dan 3D, penentuan minimal energy, pembuatan kode SMILES serta prediksi sifat fisikokimia dan toksisitas. Setelah dipreparasi, dilanjutkan proses penambatan molekul menggunakan aplikasi PyRx-Vina®. Hasil docking dianalisis dengan perangkat lunak PyMOL® dan Discovery Studio Visualizer®. Hasil profil fisikokimia menunjukkan bahwa senyawa Arbidol dan Nitazoxanide telah memenuhi hukum Lima Lipinski dan masing-masing memiliki nilai LD50 sebesar 1350 mg/kg dan 340 mg/kg. Hasil penambatan senyawa uji dengan reseptor menghasilkan interaksi yaitu ikatan hidrogen, interaksi hidrofobik dan interaksi elektrostatik. Interaksi Nitazoxanide pada reseptor 2GZ7 serta Arbidol pada reseptor 3D0G dan 1R4L menunjukkan perbedaan yang signifikan masing-masing terhadap Lopinavir dan Chloroquine dengan nilai p < 0.05, sehingga senyawa tersebut diprediksi memiliki potensi antivirus lebih baik.
El carácter secreto del testamento cerrado y la certificación notarial de haberse otorgado en varios ejemplares de idéntico tenor
Authors: Alcalde Silva Jaime , Goldenberg Serrano Juan Luis
Published: April 2022

In private wills, the legislator has tried to balance two interests that are object of legal protection: to safeguard the testator who does not wish to communicate his last will to third parties and to assure that, once it is necessary to carry out his testamentary provisions, we face the will of whom can no longer be consulted. The complexity of the formalities that normally entail the granting of private wills has motivated its disappearance in modern revisions of succession law, usually replacing it with the simplicity of the holographic will and its subsequent registration and now also by certain digital documents. The Chilean Civil Code has not yet followed such path, but practice has provided for certain variants that aim to lessen any doubt of the testator’s last will in case of loss of the document, such as its granting of several copies with the same content, circumstance that is certified by the respective Notary. Through the review of the sources and the breakdown of its legal formalities, we intend to verify that such certification is not possible without compromising the validity of the will as it affects its essential feature (the secrecy of tis provisions), urging to discard such practice and, if necessary, search for a legislative modification that grants the desired certainty to the testator.
Problematización de la investigación en nanotecnología agrícola y alimentaria en el marco de la nueva política de ciencia, tecnología e innovación en México
Authors: Anzaldo Montoya Mónica , Hernández-Adame Luis
Published: April 2022

Los debates en la literatura sobre políticas de ciencia, tecnología e innovación (CTI) argumentan la importancia de reconciliar la oferta y la demanda de conocimientos para enfrentar las desigualdades sociales. En este trabajo, problematizamos la oferta y la demanda de conocimientos científicos en el campo de la nanotecnología (NT) agrícola y alimentaria, en el contexto de un cambio en la concepción de la política de CTI que acompaña la llegada del nuevo gobierno federal en el año 2018. Para operacionalizar estas dimensiones, primero realizamos un mapeo de los proyectos de investigación aprobados por el Consejo Nacional de Ciencia y Tecnología en el periodo 2012-2019. Posteriormente, examinamos las demandas de investigación establecidas por el consejo en torno a los sistemas agroalimentarios. Con ello, discutimos las condiciones de posibilidad que tiene la NT agrícola y alimentaria para responder tales demandas. Concluimos que los cambios en la concepción de ciencia significan nuevas prioridades de investigación para los sistemas agroalimentarios del país, entre ellos, contribuir a la soberanía alimentaria y la sustitución de agroquímicos en el campo; pero más aún, plantean retos para reflexionar sobre: ¿cómo podemos hacer para que la NT agrícola y alimentaria contribuya a alcanzar una agricultura justa con los productores rurales y respetuosa con el ambiente?
Implementation of a Real-Time Vision Based Vehicle License Plate Recognition System
Authors: Gothe Arti , Kadam Prof R. D. , Chore Dr. N. M.
Published: April 2022

Abstract: Automatic Number-plate recognition is a technology that uses visual cues in images to read vehicle registration plates to create vehicle location data. It can use closed television, traffic law cameras, or cameras designed for work. ANPR is used by police around the world for legal purposes, including checking whether a vehicle is registered or licensed. It is also used for the collection of electronic toll on individual toll roads and as a catalyst for traffic mobility, for example by highway agencies. The automatic number plate recognition can be used to store camera-captured images and text from the license plate, another configurable to save the driver's image. Systems usually use infrared light to allow the camera to take pictures at any time of the day or night. An Intelligent Vehicle Safety recording system that uses image processing usually requires a large internal memory register or DDR SDRAM (Random Double Data Access) for temporary video and photos. But these processes often increase the complexity of hardware and software. In this program, we propose a VLSI implementation for a photographic route departure system. It is not only a calculation of line melting and discrimination outside the line, but also a real-time analysis of car tires on the left and right side of the track marker with a video image, and then judge the car offsets phenomenon. Keywords: ANPR, Artificial Intelligence, Government Tracking, Security etc.