bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of Different Cultivation Patterns on Amomum villosum Yield and Quality Parameters, Rhizosphere Soil Properties, and Rhizosphere Soil Microbes
Authors: Wang Butian , Chen Hongmei , Qu Peng , Lin Rong , He Suming , Li Weifeng , Zhang Chuanli , Shi Xuedong , Liu Yi , Du Huabo , Ge Yu
Published: February 2023

The forest–medicinal plant management system has benefited the commercial production of Amomum villosum. However, little is known about the influence of different forestlands on the cultivation of A. villosum. The present study investigated the potential differences in the A. villosum yield and quality parameters, rhizosphere soil properties, and rhizosphere soil microbiota between a rubber plantation (RP) and a natural secondary forest (NSF). No significant differences in yield or rhizosphere soil properties of A. villosum were observed between RP and NSF, although most of the A. villosum yield parameters, the rhizosphere soil physicochemical properties, and soil enzyme activities were higher in NSF than in RP. Furthermore, the 38 volatile components had significantly higher relative abundances in NSF than in RP. Furthermore, the alpha diversity indices for the microbiota communities in the A. villosum rhizosphere soil indicated that the richness of the bacterial and fungal communities was significantly higher in NSF than in RP. These findings suggest that NSF conditions may be more appropriate than RP conditions for growing A. villosum. The data generated in this study may be useful for increasing the production of high-quality A. villosum via the exploitation of natural environments.
Validation of the Polish Problematic Tinder Use Scale and Its Relationship with Safe Sex Behaviors
Authors: Liberacka-Dwojak Magdalena , Khazaal Yasser , Wiłkość-Dębczyńska Monika , Kukuła Daria , Chechłowska Anna , Kozłowska Aleksandra , Przywitowska Nikola , Jeannot Emilien
Published: February 2023

Introduction: Online dating is a common phenomenon. The manageability and access of the application allows people to quickly reach many potential partners, which can increase risky sexual behaviors. The Problematic Tinder Use Scale (PTUS) was developed and validated in a Polish population by analyzing the reliability, validity, and factor structure of the responses given by Polish-speaking participants. Methods: Two samples of adult Tinder users were recruited online. The first study aimed to perform the reliability coefficient Cronbach’s, interrater analysis, exploratory, and confirmatory factor analysis. The second sample was recruited to investigate the factor structure by combining it with the Safe Sex Behavior Questionnaire (SSBQ). The sociodemographic data, such as hours of use and number of dates, were also investigated. Results: The Polish participants’ responses to the PTUS (sample 1: N = 271, sample 2: N = 162) revealed the one-factor structure of the tool. The reliability of the measurement was α = 0.80. The construct validity was confirmed. The results showed a significant, negative, and weak correlation between the PTUS and SSBQ scores and their subscales: risky sexual behaviors (r = −0.18), condom use (r = −0.22), and avoidance of body fluids (r = −0.17). Moreover, the number of partners met in the real world had a statistically significant, moderate relationship with the PTUS scores. Conclusions: The PTUS measurement is valid and reliable for the Polish population. The findings highlight the need for harm prevention strategies related to potentially addictive Tinder use, as well as the possible risky sexual behaviors associated with dating app use.
Mapping Grip Force Characteristics in the Measurement of Stress in Driving
Authors: Sahar Yotam , Elbaum Tomer , Musicant Oren , Wagner Michael , Altarac Leon , Shoval Shraga
Published: February 2023

Reducing drivers’ stress can potentially increase road safety. However, state-of-the-art physiological stress indices are intrusive and limited by long time lags. Grip force is an innovative index of stress that is transparent to the user and, according to our previous findings, requires a two- to five-second time window. The aim of this study was to map the various parameters affecting the relationship between grip force and stress during driving tasks. Two stressors were used: the driving mode and the distance from the vehicle to a crossing pedestrian. Thirty-nine participants performed a driving task during either remote driving or simulated driving. A pedestrian dummy crossed the road without warning at two distances. The grip force on the steering wheel and the skin conductance response were both measured. Various model parameters were explored, including time window parameters, calculation types, and steering wheel surfaces for the grip force measurements. The significant and most powerful models were identified. These findings may aid in the development of car safety systems that incorporate continuous measurements of stress.
Differences in Drug Use among Persons Experiencing Homelessness According to Gender and Nationality
Authors: Parés-Bayerri Alícia , Calvo Fran , Font-Mayolas Sílvia , Panadero Sonia , Vázquez José Juan
Published: February 2023

The main aims of this article are to update the data related to drug and alcohol use in persons experiencing homelessness (PEH) who use shelters, and to see if there are significant differences in their drug use depending on their gender and nationality. The article presents an analysis of the interconnections between the results of drug dependence detection tools (Alcohol Use Disorders Identification Test (AUDIT), Drug Abuse Screening Test (DAST-10), Severity of Dependence Scale (SDS)) according to gender and nationality with the intention of identifying specific needs that lead to new lines of research into better approaches to homelessness. A cross-sectional, observational and analytical method was used to analyse the experiences of persons experiencing homelessness who use various shelters in the cities of Madrid, Girona, and Guadalajara (Spain). The results show that there are no gender differences in the risks of using drugs and drug addiction, but there are differences in terms of nationality for drug addiction, with Spanish nationals showing a greater tendency to develop drug addiction. These findings have significant implications, as they highlight socio-cultural and socio-educational influence as risk factors in drug addiction behaviours.
Regional Regression Correlation Model of Microplastic Water Pollution Control Using Circular Economy Tools
Authors: Antohi Valentin Marian , Ionescu Romeo Victor , Zlati Monica Laura , Iticescu Catalina , Georgescu Puiu Lucian , Calmuc Madalina
Published: February 2023

Water pollution caused by microplastics represents an important challenge for the environment and people’s health. The weak international regulations and standards in this domain support increased water pollution with microplastics. The literature is unsuccessful in establishing a common approach regarding this subject. The main objective of this research is to develop a new approach to necessary policies and ways of action to decrease water pollution caused by microplastics. In this context, we quantified the impact of European water pollution caused by microplastics in the circular economy. The main research methods used in the paper are meta-analysis, statistical analysis and an econometric approach. A new econometric model is developed in order to assist the decision makers in increasing efficiency of public policies regarding water pollution elimination. The main result of this study relies on combining, in an integrated way, the Organisation for Economic Co-operation and Development’s (OECD) data on microplastic water pollution and identifying relevant policies to combat this type of pollution.
Identifying Public Healthcare Priorities in Virtual Care for Older Adults: A Participatory Research Study
Authors: Pu Dai , Palmer Victoria , Greenstock Louise , Pigott Cathie , Peeters Anna , Sanci Lena , Callisaya Michele , Browning Colette , Chapman Wendy , Haines Terry
Published: February 2023

There has been increasing adoption and implementation of virtual healthcare in recent years, especially with COVID-19 impacting the world. As a result, virtual care initiatives may not undergo stringent quality control processes to ensure that they are appropriate to their context and meet sector needs. The two objectives of this study were to identify virtual care initiatives for older adults currently in use in Victoria and virtual care challenges that could be prioritised for further investigation and scale-up and to understand why certain virtual care initiatives and challenges are prioritised over others for investigation and scale-up. Methods: This project used an Emerging Design approach. A survey of public health services in the state of Victoria in Australia was first carried out, followed by the co-production of research and healthcare priorities with key stakeholders in the areas of primary care, hospital care, consumer representation, research, and government. The survey was used to gather existing virtual care initiatives for older adults and any associated challenges. Co-production processes consisted of individual ratings of initiatives and group-based discussions to identify priority virtual care initiatives and challenges to be addressed for future scale-up. Stakeholders nominated their top three virtual initiatives following discussions. Results: Telehealth was nominated as the highest priority initiative type for scaling up, with virtual emergency department models of care nominated as the highest priority within this category. Remote monitoring was voted as a top priority for further investigations. The top virtual care challenge was data sharing across services and settings, and the user-friendliness of virtual care platforms was nominated as the top priority for further investigation. Conclusions: Stakeholders prioritised public health virtual care initiatives that are easy to adopt and address needs that are perceived to be more immediate (acute more so than chronic care). Virtual care initiatives that incorporate more technology and integrated elements are valued, but more information is needed to inform their potential scale-up.
Current Awareness Status of and Recommendations for Polycystic Ovarian Syndrome: A National Cross-Sectional Investigation of Central Jordan
Authors: Muhaidat Nadia , Mansour Shahd , Dardas Majid , Qiqieh Jamil , Halasa Zeina , Al-Huneidy Leen , Samhouri Jehad , Rayyan Rama , AlOweiwi Wahid , AlMohtasib Jamil , Alshrouf Mohammad A. , Al-Labadi Ghayda’a M. , Suboh Layla H. , Al-Ani Abdallah
Published: February 2023

Polycystic ovary syndrome (PCOS) is a common reproductive disorder that is related to a number of health issues and has an influence on a variety of metabolic processes. Despite its burden on the health of females, PCOS is significantly underdiagnosed, which is associated with lack of disease knowledge among females. Therefore, we aimed to gauge the awareness of PCOS in both the male and female population in Jordan. A descriptive cross-sectional study was conducted, targeting individuals over the age of 18 from Jordan’s central region. Participants were recruited through stratified random sampling. The questionnaire consisted of two domains, including demographics and knowledge of PCOS domains. A total of 1532 respondents participated in this study. The findings revealed that participants have overall adequate knowledge regarding PCOS’s risk factors, etiology, clinical presentation, and outcomes. However, participants demonstrated subpar familiarity of the association between PCOS and other comorbidities and the effect of genetics on PCOS. Women had more knowledge than men about PCOS (57.5 ± 6.06 vs. 54.1 ± 6.71, p = 0.019). In addition, older, employed, and higher-income populations showed significantly better knowledge than younger, unemployed, self-employed, and lower-income populations. In conclusion, we demonstrated that Jordanian women demonstrate an acceptable yet incomplete level of knowledge towards PCOS. We recommend establishing educational programs by specialists for the general population as well as medical personnel to spread accurate medical information and clarify common misconceptions about signs, symptoms, management, and treatment of PCOS, and nutritional knowledge.
Negligible Effects of Nutraceuticals from Beetroot Extract on Cardiovascular and Autonomic Recovery Response following Submaximal Aerobic Exercise in Physically Active Healthy Males: A Randomized Trial
Authors: Benjamim Cicero Jonas R. , de Sousa Júnior Francisco Welington , Porto Andrey Alves , Andrade Camila Venancia Guerra , de Figueiredo Maria Íris L. Saraiva , Benjamim Cicera Josilânia R. , da Silva Rodrigues Guilherme , Rocha Elida M. Braga , Cavalcante Taisy Ferro , Garner David M. , Valenti Vitor Engracia , Bueno Júnior Carlos R.
Published: February 2023

Background: There is little evidence that nutraceuticals from beetroot extract are beneficial with regards to recovery of the cardiovascular parameters and the autonomic nervous system (ANS) after submaximal aerobic exercise, though this formulation is employed widely for this purpose. Objective: To study the effects of beetroot extract supplementation on the recovery of cardiorespiratory and autonomic parameters after a session of submaximal aerobic exercise. Methods: Sixteen healthy male adults commenced a cross-over, randomized, double-blind and placebo-controlled trial. Beetroot extract (600 mg) or placebo (600 mg) were ingested 120 min prior to evaluation on randomized days. We assessed systolic blood pressure (SBP), diastolic blood pressure (DBP), pulse pressure (PP), mean arterial pressure (MAP), heart rate (HR) and HR variability (HRV) indexes at Rest and during 60 min of recovery from submaximal aerobic exercise. Results: Beetroot extract ingestion slightly accelerated HR, SBP, DBP and MAP reduction following exercise associated to the placebo protocol (vs. rest). Yet no group effect (p = 0.99) was identified between the beetroot and placebo protocols on HR mean, in addition to interaction (group vs. time) (p = 0.60). No group effect was attained between the SBP (p = 0.90), DBP (p = 0.88), MAP (p = 0.73) and PP (p = 0.99) protocols and no significant differences (group vs. time) were observed between the values of SBP (p = 0.75), DBP (p = 0.79), MAP (p = 0.93) and PP (p = 0.63) between placebo and beetroot protocols. Similarly, the reoccurrence of cardiac vagal modulation after exercise via the HF (ms2) was enhanced, but not with regards to the RMSSD index. No group effect (p = 0.99) was identified for the HF (p = 0.90) and RMSSD (p = 0.67) indices. Likewise, we observed no significant differences (group vs. time) amongst the values of HF (p = 0.69) and RMSSD (p = 0.95) between the placebo and beetroot protocols. Conclusion: Whilst beetroot extract might assist the recovery of the cardiovascular and autonomic systems following submaximal aerobic exercise in healthy males, these results seem insignificant owing to minor differences between interventions and are weak clinically.
Determinants of Public Health Personnel Spending in Spain
Authors: Puerto-Casasnovas Elena , Galiana-Richart Jorge , Mastrantonio-Ramos Maria Paola , López-Muñoz Francisco , Rocafort-Nicolau Alfredo
Published: February 2023

Public health is funded with government funds gathered from tax revenues, whether national, provincial or municipal. The health system therefore suffers during economic crisis periods, whether due to disinvestment, loss of purchasing power among health care personnel or the decrease in the number of professionals. This worsens the situation, as it is necessary to cover the needs of an increasingly elderly population and with a longer life expectancy at birth. The present study intends to show a model which explains the determination of the “Public Health Personnel Expenditure” in Spain for a determined period. A multiple linear regression model was applied to the period including the years 1980–2021. Macroeconomic and demographic variables were analyzed to explain the dependent variable. Variation in health personnel expenditure: “We included those variables which presented a high or very high correlation above r > 0.6. The variables which explain the behavior of Variation in health personnel expenditure”. It was a determining factor in the present study to consider that the variables with the greatest repercussions on health policy were mainly macroeconomic variables rather than demographic variables, with the only significant demographic variable that had a specific weight lower than macroeconomic variables being “Birth Rate”. In this sense, the contribution made to the scientific literature is to establish an explanatory model so that public policy managers and states in particular can consider it in their public spending policies, bearing in mind that health expenditures in a Beveridge-style health system, as Spain has, are paid with funds drawn from tax revenues.
Personality Determinants of Exercise-Related Nutritional Behaviours among Polish Team Sport Athletes
Authors: Gacek Maria , Wojtowicz Agnieszka , Popek Adam
Published: February 2023

A proper diet increases the effectiveness of training and accelerates post-workout regeneration. One of the factors determining eating behaviour are personality traits, including those included in the Big Five model, i.e., neuroticism, extraversion, openness, agreeableness, and conscientiousness. The aim of this study was to analyse the personality determinants of peri-exercise nutritional behaviours among an elite group of Polish athletes practicing team sports. The study was conducted in a group of 213 athletes, using the author’s validated questionnaire of exercise-related nutrition behaviours and the NEO-PI-R (Neuroticism Extraversion Openness-Personality Inventory-Revised). A statistical analysis was performed using Pearson’s linear correlation and Spearman’s rank correlation coefficients as well as a multiple regression analysis, assuming a significance level of α = 0.05. It has been shown that the level of the overall index regarding normal peri-exercise eating behaviours decreased with increasing neuroticism (r = −0.18) and agreeableness (r = −0.18). An analysis of the relationship between the personality traits (sub-scales) of the Big Five model demonstrated that the overall index of proper peri-exercise nutrition decreased with the intensification of three neuroticism traits, i.e., hostility/anger (R = −0.20), impulsiveness/immoderation (R = −0.18), and vulnerability to stress/learned helplessness (R = −0.19), and four traits of agreeableness, i.e., straightforwardness/morality (R = −0.17), compliance/cooperation (R = −0.19), modesty (R = −0.14), and tendermindedness/sympathy (R = −0.15) (p < 0.05). A multiple regression analysis exhibited that the full model consisting of all the analysed personality traits explained 99% of the variance concerning the level of the proper peri-exercise nutrition index. In conclusion, the index of proper nutrition under conditions of physical effort decreases along with the intensification of neuroticism and agreeableness among Polish athletes professionally practicing team sports.
A Cluster Randomized Controlled Trial of the Dental Sealants Quality in Rural Schoolchildren Using Innovative Suction without Dental Assistance
Authors: Thitasomakul Songchai , Tianviwat Sukanya
Published: February 2023

This study examined whether the moisture control innovation (tongue and cheek retractors and saliva contamination (SS-suction)) used without dental assistance could improve the quality of dental sealant in rural Thai school children compared to a standard treatment, i.e., high power suction with dental assistance. A single blind, cluster randomized controlled trial was conducted. Participants were 15 dental nurses working in sub-district health promoting hospitals and 482 children. All dental nurses attended workshops of SS-suction and revised dental sealant procedures. Children with sound lower first permanent molar teeth were simple-randomly assigned to either an intervention or control group. The children in the intervention group were sealed with SS-suction, and the children in the control group were sealed with high power suction and dental assistance. There were 244 children in the intervention group and 238 children in the control group. Dental nurses’ satisfaction on SS-suction was record by visual analogue scale (VAS) for each tooth during treatment. After 15–18 months, caries on sealed surfaces were examined. The results showed that the median satisfaction score of SS-suction was 9 out of 10, and 17–18% children experienced uncomfortable sensation during insertion or removal. The uncomfortable feeling disappeared once the suction was in place. Caries on sealed surfaces did not differ significantly between the intervention and control groups. Caries on the occlusal surface was present in 26.7% and 27.5%, and caries on the buccal surface was present in 35.2% and 36.4% of cases in the intervention and control groups, respectively. In conclusion, dental nurses were satisfied with SS-suction in terms of both function and safety. The effectiveness of SS-suction was compatible with the standard procedure after 15–18 months.
Understanding Information Processing and Protective Behaviors during the Pandemic: A Three-Wave Longitudinal Study
Authors: Cao Weidan , Yang Qinghua , Zhang Xinyao
Published: February 2023

Background: Few existing studies have examined information processing as an independent variable to predict subsequent information behaviors in a pandemic context, and the mechanism of subsequent information behavior processing following the initial or prior information behavior is unclear. Objective: Our study aims to apply the risk information seeking and processing model to explain the mechanism of subsequent systematic information processing in the context of the COVID-19 pandemic. Methods: A three-wave longitudinal online national survey was administered during the period of July 2020 to September 2020. Path analysis was conducted to test the relationships between prior and subsequent systematic information processing and protective behaviors. Results: One important finding was the key role of prior systematic information processing, as indirect hazard experience was found to be a direct predictor of risk perception (β = 0.15, p = 0.004) and an indirect predictor of protective behaviors. Another important finding was the central role of information insufficiency as a mediator/driving force in subsequent systematic information processing and protective behavior. Conclusions: The study has made important contributions in that it extends the scholarship on health information behaviors by (a) highlighting that relevant hazard experience in risk information seeking and processing model should be expanded to include indirect experience, and (b) providing the mechanism of subsequent systematic information processing following prior information processing. Our study also provides practical implications on health/risk communication and protective behaviors’ promotion in the pandemic context.
Achieving Net Zero—An Illustration of Carbon Emissions Reduction with A New Meta-Inverse DEA Approach
Authors: Yang Wen-Chi , Lu Wen-Min
Published: February 2023

To achieve the goal of limiting global warming to 1.5 °C above preindustrial levels, net-zero emissions targets were proposed to assist countries in planning their long-term reduction. Inverse Data Envelopment Analysis (DEA) can be used to determine optimal input and output levels without sacrificing the set environmental efficiency target. However, treating countries as having the same capability to mitigate carbon emissions without considering their different developmental stages is not only unrealistic but also inappropriate. Therefore, this study incorporates a meta-concept into inverse DEA. This study adopts a three-stage approach. In the first stage, a meta-frontier DEA method is adopted to assess and compare the eco-efficiency of developed and developing countries. In the second stage, the specific super-efficiency method is adopted to rank the efficient countries specifically focused on carbon performance. In the third stage, carbon dioxide emissions reduction targets are proposed for the developed and developing countries separately. Then, a new meta-inverse DEA method is used to allocate the emissions reduction target to the inefficient countries in each of the specific groups. In this way, we can find the optimal CO2 reduction amount for the inefficient countries with unchanged eco-efficiency levels. The implications of the new meta-inverse DEA method proposed in this study are twofold. The method can identify how a DMU can reduce undesirable outputs without sacrificing the set eco-efficiency target, which is especially useful in achieving net-zero emissions since this method provides a roadmap for decision-makers to understand how to allocate the emissions reduction targets to different units. In addition, this method can be applied to heterogeneous groups where they are assigned to different emissions reduction targets.
Is Suicide a Water Justice Issue? Investigating Long-Term Drinking Water Advisories and Suicide in First Nations in Canada
Authors: Ansloos Jeffrey , Cooper Annelies
Published: February 2023

First Nations experience disproportionate rates of suicide when compared to the general population. Various risk factors are identified to increase understanding of the prevalence of suicide among First Nations, but environmental dimensions of suicide are understudied. This study asks whether water insecurity, as reflected by long-term drinking water advisories (LT-DWA), has any bearing on the distribution of suicide in First Nations across Canada, and specifically in Ontario. To assess this, we established the proportion of First Nations with LT-DWAs in Canada and in Ontario that have had suicides occur between 2011 and 2016 through a review of media archives. This proportion was compared to census data on the proportion of First Nations with suicides in Canada and in Ontario between 2011 and 2016, and statistical significance of difference was determined through chi-square goodness of fit test. Overall, the findings were mixed. Nationally, there was no significantly difference of proportion of First Nations with LT-DWAs with combined (confirmed and probable) reported suicides occurring when compared to census proportions; however, at the provincial level, findings had significant differences. The authors conclude that water insecurity in First Nations, as indicated by the presence of a LT-DWA in First Nations across may be an important environmental dimension of suicide, contributing to enhanced risk for suicide in First Nations.
Public Support for Nutrition-Related Actions by Food Companies in Australia: A Cross-Sectional Analysis of Findings from the 2020 International Food Policy Study
Authors: Yin Ebony , Cameron Adrian J. , Schultz Sally , White Christine M. , Vanderlee Lana , Hammond David , Sacks Gary
Published: February 2023

Unhealthy food environments contribute to unhealthy population diets. In Australia, the government currently relies on voluntary food company actions (e.g., related to front-of-pack labelling, restricting promotion of unhealthy foods, and product formulation) as part of their efforts to improve population diets, despite evidence that such voluntary approaches are less effective than mandatory policies. This study aimed to understand public perceptions of potential food industry nutrition-related actions in Australia. An online survey was completed by 4289 Australians in 2020 as part of the International Food Policy Study. The level of public support was assessed for six different nutrition-related actions related to food labelling, food promotion, and product formulation. High levels of support were observed for all six company actions, with the highest support observed for displaying the Health Star Rating on all products (80.4%) and restricting children’s exposure to online promotion of unhealthy food (76.8%). Findings suggest the Australian public is strongly supportive of food companies taking action to improve nutrition and the healthiness of food environments. However, given the limitations of the voluntary action from food companies, mandatory policy action by the Australian government is likely to be needed to ensure company practices align with public expectations.
Creep Characteristics of Layered Rock Masses after Water Absorption Due to Structural Effects
Authors: Xu Huichen , Sun Xiaoming , Zhang Yong , Zhao Chengwei , Miao Chengyu , Wang Dong
Published: February 2023

Affected by the “three highs and one disturbance” (high ground pressure, high ground temperature, high permeability pressure, and strong mining disturbance), deep layered rock mass roadways often display large deformations, resulting in accidents and disasters from time to time. This paper aims to study creep characteristics of layered rock masses after water absorption due to structural effects, combined with acoustic emission energy and dominant frequency value analysis. Experimental results show that as the water content decreases, the long-term strength of the rock sample increases, and the damage becomes more severe. Under the same water content state conditions, the rock samples with bedding angles of 0°, 30°, and 90° have high long-term strength and undergo severe failure, whereas rock samples with bedding angles of 45° and 60° have low long-term strength and undergo mild failure. Under the same water content, the initial energy release increases with the bedding angle. Under the same water content, the energy release during failure decreases first and then increases with the increasing bedding angle. The initial energy, the cumulative energy, the initial main frequency, and the main frequency at the time of failure tend to decrease with the increase in water content.
Prevalence of and Factors Associated with Depressive Symptoms among Indonesian Migrant Workers in Taiwan
Authors: Asri Yuni , Chuang Kun-Yang
Published: February 2023

International migrant workers contribute significantly to the economic growth of the receiving country, and yet their health, especially their mental health, has long been overlooked. The purpose of this study was to identify the factors associated with depressive symptoms among Indonesian migrant workers in Taiwan. This study used cross-sectional data from 1031 Indonesian migrant workers in Taiwan. Demographic, health, and living- and work-related variables, as well as depressive symptom variables assessed using the Center for Epidemiological Study on depressive symptoms scale, were collected. Logistic regression analysis was used to identify related factors. About 15% of the Indonesian migrant workers had depressive symptoms. The significant factors associated with these symptoms were age, educational level, frequency of contact with families, self-rated health, time spent in Taiwan, region of work, satisfaction with the living environment, and freedom to go out after work. The findings thus identify target groups who are more likely than others to suffer from depressive symptoms, and we suggest appropriate approaches for devising interventions to reduce depressive symptoms. The findings of this research suggest the need for targeted approaches to reducing depressive symptoms among this population group.
Cyanidin-3-O-Glucoside Rescues Zearalenone-Induced Apoptosis via the ITGA7-PI3K-AKT Signaling Pathway in Porcine Ovarian Granulosa Cells
Authors: Li Xiuxiu , Wang Jingya , Zhang Fali , Yu Mubin , Zuo Ning , Li Lan , Tan Jinghe , Shen Wei
Published: February 2023

Zearalenone (ZEN) is an important secondary metabolite of Fusarium fungi, exposure to which can cause reproductive disorders through its effects on ovarian granulosa cells (GCs) in many mammals, especially in pigs. This study aimed to investigate the protective effects of Cyanidin-3-O-glucoside (C3G) on the ZEN-induced negative effects in porcine GCs (pGCs). The pGCs were treated with 30 µM ZEN and/or 20 µM C3G for 24 h; they were divided into a control (Ctrl) group, ZEN group, ZEN+C3G (Z+C) group, and a C3G group. Bioinformatics analysis was used to systematically screen differentially expressed genes (DEGs) in the rescue process. Results showed that C3G could effectively rescue ZEN-induced apoptosis in pGCs, and notably increase cell viability and proliferation. Furthermore, 116 DEGs were identified, and the phosphatidylinositide 3-kinases-protein kinase B (PI3K-AKT) signaling pathway was the center of attention, of which five genes and the PI3K-AKT signaling pathway were confirmed by real-time quantitative PCR (qPCR) and/or Western blot (WB). As analyzed, ZEN inhibited mRNA and protein levels of integrin subunit alpha-7 (ITGA7), and promoted the expression of cell cycle inhibition kinase cyclin-D3 (CCND3) and cyclin-dependent kinase inhibitor 1 (CDKN1A). After the knock-down of ITGA7 by siRNA, the PI3K-AKT signaling pathway was significantly inhibited. Meanwhile, proliferating cell nuclear antigen (PCNA) expression decreased, and apoptosis rates and pro-apoptotic proteins increased. In conclusion, our study demonstrated that C3G exhibited significant protective effects on the ZEN-induced inhibition of proliferation and apoptosis via the ITGA7-PI3K-AKT pathway.
Renoprotective Impacts of Inonotus obliquus Ethanol-Ethyl Acetate Extract on Combined Streptozotocin and Unilateral Nephrectomy-Induced Diabetic Nephropathy in Mice
Authors: Chiang Kuang-Hsing , Chiu Yi-Chun , Yar Noi , Chen Yu-Chun , Cheng Chia-Hui , Liu Yi-Chien , Chang Chia-Yu , Chuu Jiunn-Jye
Published: February 2023

Diabetes nephropathy (DN) is one of the most common causes of end stage renal disease (ESRD) globally. Medication options to stop or slow the progression of chronic renal disease (CKD) are limited, and patients with DN remain at a high risk of developing renal failure. Inonotus obliquus extracts (IOEs) of Chaga mushroom have been shown to have anti-glycemic, anti-hyperlipidemia, antioxidant, and anti-inflammatory effects against diabetes. In this study, we examined the potential renal protective role of an ethyl acetate layer after water-ethyl acetate separation from Inonotus obliquus ethanol crude extract (EtCE-EA) from Chaga mushrooms in diabetic nephropathy mice after preparation with 1/3 NT + STZ. Our data showed that treatment with EtCE-EA can effectively regulate blood glucose, albumin-creatinine ratio, serum creatinine, and blood urea nitrogen (BUN) levels, and it can improve the renal damage in 1/3 NT + STZ-induced CRF mice with an increase in concentration (100, 300, and 500 mg/kg). In the immunohistochemical staining test, EtCE-EA can effectively reduce the expression of TGF-β and α-SMA after induction according to the increase in the concentration (100 mg/kg, 300 mg/kg), thereby slowing down the degree of kidney damage. Our findings demonstrate that EtCE-EA could provide renal protection in diabetes nephropathy, possibly due to the decreased expression of transforming growth factor-β1 and α-smooth muscle actin.
Investigating a Library of Flavonoids as Potential Inhibitors of a Cancer Therapeutic Target MEK2 Using in Silico Methods
Authors: AlZahrani Wejdan M. , AlGhamdi Shareefa A. , Sohrab Sayed S. , Rehan Mohd
Published: February 2023

The second leading cause of death in the world is cancer. Mitogen-activated protein kinase (MAPK) and extracellular signal-regulated protein kinase (ERK) 1 and 2 (MEK1/2) stand out among the different anticancer therapeutic targets. Many MEK1/2 inhibitors are approved and widely used as anticancer drugs. The class of natural compounds known as flavonoids is well-known for their therapeutic potential. In this study, we focus on discovering novel inhibitors of MEK2 from flavonoids using virtual screening, molecular docking analyses, pharmacokinetic prediction, and molecular dynamics (MD) simulations. A library of drug-like flavonoids containing 1289 chemical compounds prepared in-house was screened against the MEK2 allosteric site using molecular docking. The ten highest-scoring compounds based on docking binding affinity (highest score: −11.3 kcal/mol) were selected for further analysis. Lipinski’s rule of five was used to test their drug-likeness, followed by ADMET predictions to study their pharmacokinetic properties. The stability of the best-docked flavonoid complex with MEK2 was examined for a 150 ns MD simulation. The proposed flavonoids are suggested as potential inhibitors of MEK2 and drug candidates for cancer therapy.
ZnO Tetrapods for Label-Free Optical Biosensing: Physicochemical Characterization and Functionalization Strategies
Authors: Terracciano Monica , Račkauskas Simas , Falanga Andrea Patrizia , Martino Sara , Chianese Giovanna , Greco Francesca , Piccialli Gennaro , Viscardi Guido , De Stefano Luca , Oliviero Giorgia , Borbone Nicola , Rea Ilaria
Published: February 2023

In this study, we fabricated three different ZnO tetrapodal nanostructures (ZnO-Ts) by a combustion process and studied their physicochemical properties by different techniques to evaluate their potentiality for label-free biosensing purposes. Then, we explored the chemical reactivity of ZnO-Ts by quantifying the available functional hydroxyl groups (–OH) on the transducer surface necessary for biosensor development. The best ZnO-T sample was chemically modified and bioconjugated with biotin as a model bioprobe by a multi-step procedure based on silanization and carbodiimide chemistry. The results demonstrated that the ZnO-Ts could be easily and efficiently biomodified, and sensing experiments based on the streptavidin target detection confirmed these structures’ suitability for biosensing applications.
Plant Protection against Viruses: An Integrated Review of Plant Immunity Agents
Authors: Huang Min , Wu Zilin , Li Jingxin , Ding Yuyu , Chen Shilin , Li Xiangyang
Published: February 2023

Plant viruses are an important class of pathogens that seriously affect plant growth and harm crop production. Viruses are simple in structure but complex in mutation and have thus always posed a continuous threat to agricultural development. Low resistance and eco-friendliness are important features of green pesticides. Plant immunity agents can enhance the resilience of the immune system by activating plants to regulate their metabolism. Therefore, plant immune agents are of great importance in pesticide science. In this paper, we review plant immunity agents, such as ningnanmycin, vanisulfane, dufulin, cytosinpeptidemycin, and oligosaccharins, and their antiviral molecular mechanisms and discuss the antiviral applications and development of plant immunity agents. Plant immunity agents can trigger defense responses and confer disease resistance to plants, and the development trends and application prospects of plant immunity agents in plant protection are analyzed in depth.
Antimicrobial and Antibiofilm Activities of Carvacrol, Amoxicillin and Salicylhydroxamic Acid Alone and in Combination vs. Helicobacter pylori: Towards a New Multi-Targeted Therapy
Authors: Puca Valentina , Turacchio Gabriele , Marinacci Beatrice , Supuran Claudiu T. , Capasso Clemente , Di Giovanni Pamela , D’Agostino Ilaria , Carradori Simone , Grande Rossella
Published: February 2023

The World Health Organization has indicated Helicobacter pylori as a high-priority pathogen whose infections urgently require an update of the antibacterial treatments pipeline. Recently, bacterial ureases and carbonic anhydrases (CAs) were found to represent valuable pharmacological targets to inhibit bacterial growth. Hence, we explored the underexploited possibility of developing a multiple-targeted anti-H. pylori therapy by assessing the antimicrobial and antibiofilm activities of a CA inhibitor, carvacrol (CAR), amoxicillin (AMX) and a urease inhibitor (SHA), alone and in combination. Minimal Inhibitory (MIC) and Minimal Bactericidal (MBC) Concentrations of their different combinations were evaluated by checkerboard assay and three different methods were employed to assess their capability to eradicate H. pylori biofilm. Through Transmission Electron Microscopy (TEM) analysis, the mechanism of action of the three compounds alone and together was determined. Interestingly, most combinations were found to strongly inhibit H. pylori growth, resulting in an additive FIC index for both CAR-AMX and CAR-SHA associations, while an indifferent value was recorded for the AMX-SHA association. Greater antimicrobial and antibiofilm efficacy of the combinations CAR-AMX, SHA-AMX and CAR-SHA against H. pylori were found with respect to the same compounds used alone, thereby representing an innovative and promising strategy to counteract H. pylori infections.
Proteomics and Its Applications in Cancers
Authors: Naryzhny Stanislav
Published: February 2023

Cancer is a system malignant transformation that covers a wide group of diseases and can affect any organ of the human body [...]
Peripheral and Central Iron Measures in Alcohol Use Disorder and Aging: A Quantitative Susceptibility Mapping Pilot Study
Authors: Adams Aiden R. , Li Xinyi , Byanyima Juliana I. , Vesslee Sianneh A. , Nguyen Thanh D. , Wang Yi , Moon Brianna , Pond Timothy , Kranzler Henry R. , Witschey Walter R. , Shi Zhenhao , Wiers Corinde E.
Published: February 2023

Chronic excessive alcohol use has neurotoxic effects, which may contribute to cognitive decline and the risk of early-onset dementia. Elevated peripheral iron levels have been reported in individuals with alcohol use disorder (AUD), but its association with brain iron loading has not been explored. We evaluated whether (1) serum and brain iron loading are higher in individuals with AUD than non-dependent healthy controls and (2) serum and brain iron loading increase with age. A fasting serum iron panel was obtained and a magnetic resonance imaging scan with quantitative susceptibility mapping (QSM) was used to quantify brain iron concentrations. Although serum ferritin levels were higher in the AUD group than in controls, whole-brain iron susceptibility did not differ between groups. Voxel-wise QSM analyses revealed higher susceptibility in a cluster in the left globus pallidus in individuals with AUD than controls. Whole-brain iron increased with age and voxel-wise QSM indicated higher susceptibility with age in various brain areas including the basal ganglia. This is the first study to analyze both serum and brain iron loading in individuals with AUD. Larger studies are needed to examine the effects of alcohol use on iron loading and its associations with alcohol use severity, structural and functional brain changes, and alcohol-induced cognitive impairments.
Abusive Head Trauma Animal Models: Focus on Biomarkers
Authors: Nikam Rahul M. , Kecskemethy Heidi H. , Kandula Vinay V. R. , Averill Lauren W. , Langhans Sigrid A. , Yue Xuyi
Published: February 2023

Abusive head trauma (AHT) is a serious traumatic brain injury and the leading cause of death in children younger than 2 years. The development of experimental animal models to simulate clinical AHT cases is challenging. Several animal models have been designed to mimic the pathophysiological and behavioral changes in pediatric AHT, ranging from lissencephalic rodents to gyrencephalic piglets, lambs, and non-human primates. These models can provide helpful information for AHT, but many studies utilizing them lack consistent and rigorous characterization of brain changes and have low reproducibility of the inflicted trauma. Clinical translatability of animal models is also limited due to significant structural differences between developing infant human brains and the brains of animals, and an insufficient ability to mimic the effects of long-term degenerative diseases and to model how secondary injuries impact the development of the brain in children. Nevertheless, animal models can provide clues on biochemical effectors that mediate secondary brain injury after AHT including neuroinflammation, excitotoxicity, reactive oxygen toxicity, axonal damage, and neuronal death. They also allow for investigation of the interdependency of injured neurons and analysis of the cell types involved in neuronal degeneration and malfunction. This review first focuses on the clinical challenges in diagnosing AHT and describes various biomarkers in clinical AHT cases. Then typical preclinical biomarkers such as microglia and astrocytes, reactive oxygen species, and activated N-methyl-D-aspartate receptors in AHT are described, and the value and limitations of animal models in preclinical drug discovery for AHT are discussed.
A Swedish Familial Genome-Wide Haplotype Analysis Identified Five Novel Breast Cancer Susceptibility Loci on 9p24.3, 11q22.3, 15q11.2, 16q24.1 and Xq21.31
Authors: Barnekow Elin , Hasslow Johan , Liu Wen , Bryant Patrick , Thutkawkorapin Jessada , Wendt Camilla , Czene Kamila , Hall Per , Margolin Sara , Lindblom Annika
Published: February 2023

Most breast cancer heritability is unexplained. We hypothesized that analysis of unrelated familial cases in a GWAS context could enable the identification of novel susceptibility loci. In order to examine the association of a haplotype with breast cancer risk, we performed a genome-wide haplotype association study using a sliding window analysis of window sizes 1–25 SNPs in 650 familial invasive breast cancer cases and 5021 controls. We identified five novel risk loci on 9p24.3 (OR 3.4; p 4.9 × 10−11), 11q22.3 (OR 2.4; p 5.2 × 10−9), 15q11.2 (OR 3.6; p 2.3 × 10−8), 16q24.1 (OR 3; p 3 × 10−8) and Xq21.31 (OR 3.3; p 1.7 × 10−8) and confirmed three well-known loci on 10q25.13, 11q13.3, and 16q12.1. In total, 1593 significant risk haplotypes and 39 risk SNPs were distributed on the eight loci. In comparison with unselected breast cancer cases from a previous study, the OR was increased in the familial analysis in all eight loci. Analyzing familial cancer cases and controls enabled the identification of novel breast cancer susceptibility loci.
Dissecting the Meiotic Recombination Patterns in a Brassica napus Double Haploid Population Using 60K SNP Array
Authors: Yan Shuxiang , He Jianjie , Tang Mi , Ming Bangfa , Li Huaixin , Fan Shipeng , Xiong Yiyi , Chao Hongbo , Zhang Libin , Wang Aihua , Li Maoteng
Published: February 2023

Meiotic recombination not only maintains the stability of the chromosome structure but also creates genetic variations for adapting to changeable environments. A better understanding of the mechanism of crossover (CO) patterns at the population level is useful for crop improvement. However, there are limited cost-effective and universal methods to detect the recombination frequency at the population level in Brassica napus. Here, the Brassica 60K Illumina Infinium SNP array (Brassica 60K array) was used to systematically study the recombination landscape in a double haploid (DH) population of B. napus. It was found that COs were unevenly distributed across the whole genome, and a higher frequency of COs existed at the distal ends of each chromosome. A considerable number of genes (more than 30%) in the CO hot regions were associated with plant defense and regulation. In most tissues, the average gene expression level in the hot regions (CO frequency of greater than 2 cM/Mb) was significantly higher than that in the regions with a CO frequency of less than 1 cM/Mb. In addition, a bin map was constructed with 1995 recombination bins. For seed oil content, Bin 1131 to 1134, Bin 1308 to 1311, Bin 1864 to 1869, and Bin 2184 to 2230 were identified on chromosomes A08, A09, C03, and C06, respectively, which could explain 8.5%, 17.3%, 8.6%, and 3.9% of the phenotypic variation. These results could not only deepen our understanding of meiotic recombination in B. napus at the population level, and provide useful information for rapeseed breeding in the future, but also provided a reference for studying CO frequency in other species.