bluuebunny/crossref_metadata_2025 huggingface dataset:
Induction of Transgene Suppression in Plants via External Application of Synthetic dsRNA
Authors: Dubrovina Alexandra S. , Aleynova Olga A. , Kalachev Alexander V. , Suprun Andrey R. , Ogneva Zlata V. , Kiselev Konstantin V.
Published: March 2019

Recent investigations show that exogenously applied small interfering RNAs (siRNA) and long double-stranded RNA (dsRNA) precursors can be taken up and translocated in plants to induce RNA interference (RNAi) in the plant or in its fungal pathogen. The question of whether genes in the plant genome can undergo suppression as a result of exogenous RNA application on plant surface is almost unexplored. This study analyzed whether it is possible to influence transcript levels of transgenes, as more prone sequences to silencing, in Arabidopsis genome by direct exogenous application of target long dsRNAs. The data revealed that in vitro synthesized dsRNAs designed to target the gene coding regions of enhanced green fluorescent protein (EGFP) or neomycin phosphotransferase II (NPTII) suppressed their transcript levels in Arabidopsis. The fact that, simple exogenous application of polynucleotides can affect mRNA levels of plant transgenes, opens new opportunities for the development of new scientific techniques and crop improvement strategies.
Transcriptomic Sequencing and Co-Expression Network Analysis on Key Genes and Pathways Regulating Nitrogen Use Efficiency in Myriophyllum aquaticum
Authors: Wang Rui , Xu Shengjun , Jiang Cancan , Sun Haishu , Feng Shugeng , Zhou Sining , Zhuang Guoqiang , Bai Zhihui , Zhuang Xuliang
Published: March 2019

Massively input and accumulated ammonium is one of the main causes of eutrophication in aquatic ecosystems, which severely deteriorates water quality. Previous studies showed that one of the commonly used macrophytes, Myriophyllum aquaticum, was capable of not only withstanding ammonium of high concentration, but also efficiently assimilating extracellular ammonium to constitutive amino acids and proteins. However, the genetic mechanism regulating such efficient nitrogen metabolism in M. aquaticum is still poorly understood. Therefore, RNA-based analysis was performed in this study to understand the ammonium regulatory mechanism in M. aquaticum in response to various concentrations of ammonium. A total of 7721 genes were differentially expressed, of which those related to nitrogen-transport, assimilation, and remobilization were highly-regulated in response to various concentrations of ammonium. We have also identified transcription factors and protein kinases that were rapidly induced in response to ammonium, which suggests their involvement in ammonium-mediated signalling. Meanwhile, secondary metabolism including phenolics and anthocyanins biosynthesis was also activated in response to various concentrations of ammonium, especially at high ammonium concentrations. These results proposed a complex physiological and genetic regulation network related to nitrogen, carbohydrate, transcription factors, and secondary metabolism for nitrogen use efficiency in M. aquaticum.
Towards A New Approach for the Description of Cyclo–2,4-Dihydroxybenzoate, A Substance Which Effectively Mimics Zearalenone in Imprinted Polymers Designed for Analyzing Selected Mycotoxins in Urine
Authors: Gadzała-Kopciuch Renata , Kwaśniewska Katarzyna , Ludwiczak Agnieszka , Skrzyniarz Piotr , Jakubowski Rafał , Nowak Wiesław , Wojtczak Andrzej , Buszewski Bogusław
Published: March 2019

A method of purifying cyclododecyl 2,4-dihydroxybenzoate as a potential replacement template molecule for preparation of molecularly-imprinted polymers for isolation of zearalenone in urine was developed. Full physicochemical characteristics of cyclododecyl 2,4-dihydroxybenzoate for the first time included crystallographic analysis and molecular modelling, which made possible the determination of the similarity between the cyclododecyl 2,4-dihydroxybenzoate and zearalenone molecules. The obtained molecularly-imprinted polymers show very high in vitro selectivity towards zearalenone due to specific interactions (e.g., hydrogen bonding, molecular recognition interaction). The achieved extraction recovery exceeds 94% at the tested concentration levels (20–500 ng·mL−1) with a relative standard deviation below 2%. Immunosorbents were found to have lower recoveries (below 92.5%) and RSD value between 2 and 4% for higher concentrations of the studied substance (400 ng·mL−1).
Symbiotic Plant Biomass Decomposition in Fungus-Growing Termites
Authors: da Costa Rafael R. , Hu Haofu , Li Hongjie , Poulsen Michael
Published: March 2019

Termites are among the most successful animal groups, accomplishing nutrient acquisition through long-term associations and enzyme provisioning from microbial symbionts. Fungus farming has evolved only once in a single termite sub-family: Macrotermitinae. This sub-family has become a dominant decomposer in the Old World; through enzymatic contributions from insects, fungi, and bacteria, managed in an intricate decomposition pathway, the termites obtain near-complete utilisation of essentially any plant substrate. Here we review recent insights into our understanding of the process of plant biomass decomposition in fungus-growing termites. To this end, we outline research avenues that we believe can help shed light on how evolution has shaped the optimisation of plant-biomass decomposition in this complex multipartite symbiosis.
Scanning Electron Microscopy of the Antennal Sensilla and Their Secretion Analysis in Adults of Aromia bungii (Faldermann, 1835) (Coleoptera, Cerambycidae)
Authors: Di Palma Antonella , Pistillo Marco , Griffo Raffaele , Garonna Antonio P. , Germinara Giacinto S.
Published: March 2019

Background: It has been proved that chemical signals play an important role in mating location and reproductive behavior in cerambycids; moreover, they rely on contact chemoreception for mate recognition. Methods: Adult antennae of Aromia bungii were observed using scanning electron microscopy and adult antennal secretions were collected and analyzed with gas chromatography-mass spectrometry. Results: Twelve different types of sensilla were morphologically described on the antennae of A. bungii. At least six mechanoreceptors—one gustative, one putative chemo- or thermoreceptor, and three multiporous olfactory receptors—are present on the antennae of both sexes while a receptor-type of unclear function is limited to males. Secretions associated with sensilla basiconica were observed for the first time in a cerambycid species. Conclusions: Sensilla basiconica should play a role in odor perception detecting host tree volatiles and/or pheromones. Sensilla basiconica type 1 and 2 produce a viscous material accumulating on the antennal surface. Chemical analysis of adult antennal secretions highlighted marked differences between sexes. Some of the identified compounds have been previously reported as contact pheromone components of other cerambycid species. Our observations strongly suggest sensilla basiconica as the production sites of compounds involved in mate recognition.
Aseptic Rearing and Infection with Gut Bacteria Improve the Fitness of Transgenic Diamondback Moth, Plutella xylostella
Authors: Somerville Jasmine , Zhou Liqin , Raymond Ben
Published: March 2019

Mass insect rearing can have a range of applications, for example in biological control of pests. The competitive fitness of released insects is extremely important in a number of applications. Here, we investigated how to improve the fitness of a transgenic diamondback moth, which has shown variation in mating ability when reared in different insectaries. Specifically we tested whether infection with a gut bacteria, Enterobacter cloacae, and aseptic rearing of larvae could improve insect growth and male performance. All larvae were readily infected with E. cloacae. Under aseptic rearing, pupal weights were reduced and there was a marginal reduction in larval survival. However, aseptic rearing substantially improved the fitness of transgenic males. In addition, under aseptic rearing, inoculation with E. cloacae increased pupal weights and male fitness, increasing the proportion of transgenic progeny from 20% to 30% relative to uninfected insects. Aseptic conditions may improve the fitness of transgenic males by excluding microbial contaminants, while symbiont inoculation could further improve fitness by providing additional protection against infection, or by normalizing insect physiology. The simple innovation of incorporating antibiotic into diet, and inoculating insects with symbiotic bacteria that are resistant to that antibiotic, could provide a readily transferable tool for other insect rearing systems.
Electroless-Deposited Platinum Antennas for Wireless Surface Acoustic Wave Sensors
Authors: Brachmann Erik , Seifert Marietta , Neumann Niels , Alshwawreh Nidal , Uhlemann Margitta , Menzel Siegfried Bernhard , Acker Jörg , Herold Steven , Hoffmann Volker , Gemming Thomas
Published: March 2019

In an effort to develop a cost-efficient technology for wireless high-temperature surface acoustic wave sensors, this study presents an evaluation of a combined method that integrates physical vapor deposition with electroless deposition for the fabrication of platinum-based planar antennas. The proposed manufacturing process becomes attractive for narrow, thick, and sparse metallizations for antennas in the MHz to GHz frequency range. In detail, narrow platinum-based lines of a width down to 40 μm were electroless-deposited on γ-Al 2O 3 substrates using different seed layers. At first, the electrolyte chemistry was optimized to obtain the highest deposition rate. Films with various thickness were prepared and the electrical resistivity, microstructure, and chemical composition in the as-prepared state and after annealing at temperatures up to 1100 ∘C were evaluated. Using these material parameters, the antenna was simulated with an electromagnetic full-wave simulation tool and then fabricated. The electrical parameters, including the S-parameters of the antenna, were measured. The agreement between the simulated and the realized antenna is then discussed.
Asymmetric Transmission in a Mie-Based Dielectric Metamaterial with Fano Resonance
Authors: Wang Xiaobo , Li Haohua , Zhou Ji
Published: March 2019

Chiral metamaterials with asymmetric transmission can be applied as polarization-controlled devices. Here, a Mie-based dielectric metamaterial with a spacer exhibiting asymmetric transmission of linearly polarized waves at microwave frequencies was designed and demonstrated numerically. The unidirectional characteristic is attributed to the chirality of the metamolecule and the mutual excitation of the Mie resonances. Field distributions are simulated to investigate the underlying physical mechanism. Fano-type resonances emerge near the Mie resonances of the constituents and come from the destructive interference inside the structure. The near-field coupling further contributes to the asymmetric transmission. The influences of the lattice constant and the spacer thickness on the asymmetric characteristics were also analyzed by parameter sweeps. The proposed Mie-based metamaterial is of a simple structure, and it has the potential for applications in dielectric metadevices, such as high-performance polarization rotators.
Diffractive Efficiency Optimization in Metasurface Design via Electromagnetic Coupling Compensation
Authors: Li Yang , Hong Minghui
Published: March 2019

Metasurface is an advanced flat optical component that can flexibly manipulate the electromagnetic wave in an ultrathin dimension. However, electromagnetic coupling among neighbored optical elements decreases the diffractive efficiency and increases the noise. In this paper, a novel computational method is proposed to optimize the coupling of the metasurface. The coupled electric fields in metasurface design are decomposed into various coupling orders and then restructured to replace the whole metasurface simulation. This method is applied to optimize a metasurface that consisted of conventional nanorod plasmonic antennas as a case study. The convergence of this method in calculation is demonstrated. The electric field intensity deviation of a nanoantenna array can be reduced from 112.2% to 0.5% by the second-order coupling correction. The diffractive efficiency of a three-level phase meta-deflector is optimized from 73% to 86% by optimized coupling compensation via particle swarm optimization (PSO). This process opens a new area of metasurface design by the detailed field distribution of optical elements.
Development of a Novel Biosensor Based on Tyrosinase/Platinum Nanoparticles/Chitosan/Graphene Nanostructured Layer with Applicability in Bioanalysis
Authors: Apetrei Irina Mirela , Apetrei Constantin
Published: March 2019

The present paper describes the preparation and characterization of a graphene, chitosan, platinum nanoparticles and tyrosinase-based bionanocomposite film deposited on the surface of a screen-printed carbon electrode for the detection of L-tyrosine by voltammetry. The redox process on the biosensor surface is associated with the enzymatic oxidation of L-tyrosine, which is favoured by graphene and platinum nanoparticles that increase electrical conductivity and the electron transfer rate. Chitosan ensures the biocompatibility between the tyrosinase enzyme and the solid matrix, as well as a series of complex interactions for an efficient immobilization of the biocatalyst. Experimental conditions were optimized so that the analytical performances of the biosensor were maximal for L-tyrosine detection. By using square wave voltammetry as the detection method, a very low detection limit (4.75 × 10−8 M), a vast linearity domain (0.1–100 μM) and a high affinity of the enzyme for the substrate (KMapp is 53.4 μM) were obtained. The repeatability of the voltammetric response, the stability, and the reduced interference of the chemical species present in the sample prove that this biosensor is an excellent tool to be used in bioanalysis. L-tyrosine detection in medical and pharmaceutical samples was performed with very good results, the analytical recovery values obtained being between 99.5% and 101%. The analytical method based on biosensor was validated by the standard method of analysis, the differences observed being statistically insignificant at the 99% confidence level.
Atomic Simulations of Grain Structures and Deformation Behaviors in Nanocrystalline CoCrFeNiMn High-Entropy Alloy
Authors: Hou Junling , Li Qiang , Wu Chuanbao , Zheng Limei
Published: March 2019

Using the molecular dynamics method, the melting character, mechanical properties, microstructures, and strain deformation mechanisms of nanocrystalline CoCrFeNiMn high-entropy alloy are systematically investigated in the present work. The simulation results suggest that the melting point in CoCrFeNiMn high-entropy alloy decreases with the grain size, decreasing from 3.6 to 2.0 nm. The grain size has a significant effect on shear and Young’s modulus compared to bulk modulus. The stress-strain simulation demonstrates that the ultimate tensile strength decreases with the decrease of the grain size, while the plastic deformation increases with the decrease in grain size. While the average grain size decreases to 2.0 nm, the amorphization induced by small grain size reduces plastic deformation. The common neighbor analysis shows that the face-centered cubic (FCC) composition of CoCrFeNiMn decreases gradually with decreasing grain size. For the sample with a grain size of 2.0 nm, the FCC composition is about 19% at a strain of 20%, accompanied by severe amorphization. The inverse Hall-Petch effect is observed for nanocrystalline CoCrFeNiMn high-entropy alloy in the present simulations. The atomic snapshot of CoCrFeNiMn with a grain size of 2.0 nm under the uniaxial strain confirms that the grain shape change, stacking fault formation, and amorphization are important mechanisms of plastic deformation in nanocrystalline high-entropy CoCrFeNiMn.
Critical Damage Values of R200 and 100Cr6 Steels Obtained by Hot Tensile Testing
Authors: Pater Zbigniew , Gontarz Andrzej
Published: March 2019

The paper proposes a new method for determining critical damage values in hot forming processes. The method first involves performing tensile tests of axisymmetric samples and then simulating these tests numerically. Simulations are performed by the finite element method in a three-dimensional state of strain, including thermal phenomena occurring in the forming zone. The elaborated method is universal and can be used for different materials. The study is performed for two steel grades, i.e., R200 railway steel and 100Cr6 bearing steel. The results demonstrate that critical damage values strongly depend on the forming temperature.
Bioactivity of Bioceramic Materials Used in the Dentin-Pulp Complex Therapy: A Systematic Review
Authors: Sanz José Luis , Rodríguez-Lozano Francisco Javier , Llena Carmen , Sauro Salvatore , Forner Leopoldo
Published: March 2019

Dentistry-applied bioceramic materials are ceramic materials that are categorized as bioinert, bioactive and biodegradable. They share a common characteristic of being specifically designed to fulfil their function; they are able to act as root canal sealers, cements, root repair or filling materials. Bioactivity is only attributed to those materials which are capable of inducing a desired tissue response from the host. The aim of this study is to present a systematic review of available literature investigating bioactivity of dentistry-applied bioceramic materials towards dental pulp stem cells, including a bibliometric analysis of such a group of studies and a presentation of the parameters used to assess bioactivity, materials studied and a summary of results. The research question, based on the PICO model, aimed to assess the current knowledge on dentistry-based bioceramic materials by exploring to what extent they express bioactive properties in in vitro assays and animal studies when exposed to dental pulp stem cells, as opposed to a control or compared to different bioceramic material compositions, for their use in the dentin-pulp complex therapy. A systematic search of the literature was performed in six databases, followed by article selection, data extraction, and quality assessment. Studies assessing bioactivity of one or more bioceramic materials (both commercially available or novel/experimental) towards dental pulp stem cells (DPSCs) were included in our review. A total of 37 articles were included in our qualitative review. Quantification of osteogenic, odontogenic and angiogenic markers using reverse transcriptase polymerase chain reaction (RT-PCR) is the prevailing method used to evaluate bioceramic material bioactivity towards DPSCs in the current investigative state, followed by alkaline phosphatase (ALP) enzyme activity assays and Alizarin Red Staining (ARS) to assess mineralization potential. Mineral trioxide aggregate and Biodentine are the prevalent reference materials used to compare with newly introduced bioceramic materials. Available literature compares a wide range of bioceramic materials for bioactivity, consisting mostly of in vitro assays. The desirability of this property added to the rapid introduction of new material compositions makes this subject a clear candidate for future research.
On the Interplay of DLIP and LIPSS Upon Ultra-Short Laser Pulse Irradiation
Authors: Alamri Sabri , Fraggelakis Fotis , Kunze Tim , Krupop Benjamin , Mincuzzi Girolamo , Kling Rainer , Lasagni Andrés Fabián
Published: March 2019

Controlling laser induced surface morphology is essential for developing specialized functional surfaces. This work presents novel, multi-scale periodic patterns with two-dimensional symmetry generated on stainless steel, polyimide and sapphire. The microstructures were realized by combining Direct Laser Interference Patterning with the generation of Laser Induced Periodic Surface Structures in a one-step process. An industrial, fiber femtosecond laser source emitting at 1030 nm with a pulse duration of 500 fs was utilized for the experiments. In the case of stainless steel, it was possible to create line-like or pillar-like surface patterns by rotating the polarization orientation with respect to the interference pattern. In the case of polyimide and sapphire, the absorption of the laser radiation was promoted by a multiphoton mechanism. In polyimide, grooves and pillars of several microns in depth were produced over an area much larger than the spot size. Finally, for sapphire, the simultaneous generation of interference-like pattern and laser induced periodic surface structures was realized. The results reported here provide valuable data on the feasibility to combine two state-of-the-art techniques with an industrial apparatus, to control the induced surface morphology.
Photodegradation of Microcystin-LR Using Visible Light-Activated C/N-co-Modified Mesoporous TiO2 Photocatalyst
Authors: Khedr Tamer M. , El-Sheikh Said M. , Ismail Adel A. , Kowalska Ewa , Bahnemann Detlef W.
Published: March 2019

Microcystin-LR (MC-LR), a potent hepatotoxin produced by the cyanobacteria, is of increasing concern worldwide because of severe and persistent impacts on humans and animals by inhalation and consumption of contaminated waters and food. In this work, MC-LR was removed completely from aqueous solution using visible-light-active C/N-co-modified mesoporous anatase/brookite TiO2 photocatalyst. The co-modified TiO2 nanoparticles were synthesized by a one-pot hydrothermal process, and then calcined at different temperatures (300, 400, and 500 °C). All the obtained TiO2 powders were analyzed by X-ray diffraction (XRD), Raman spectroscopy, transmission electron microscope (TEM), specific surface area (SSA) measurements, ultraviolet-visible diffuse reflectance spectra (UV-vis DRS), X-ray photoelectron spectroscopy (XPS), Fourier transform infrared (FTIR) spectroscopy, and photoluminescence (PL) analysis. It was found that all samples contained mixed-phase TiO2 (anatase and brookite), and the content of brookite decreased with an increase in calcination temperature, as well as the specific surface area and the content of non-metal elements. The effects of initial pH value, the TiO2 content, and MC-LR concentration on the photocatalytic activity were also studied. It was found that the photocatalytic activity of the obtained TiO2 photocatalysts declined with increasing temperature. The complete degradation (100%) of MC-LR (10 mg L−1) was observed within 3 h, using as-synthesized co-modified TiO2 (0.4 g L−1) at pH 4 under visible light. Based on the obtained results, the mechanism of MC-LR degradation has been proposed.
Potential of Chemical and Physical Enhancers for Transungual Delivery of Amorolfine Hydrochloride
Authors: Šveikauskaitė Indrė , Pockevičius Alius , Briedis Vitalis
Published: March 2019

Topical monotherapy of nail infection is limited by poor drug permeability into the human nail plate. Numerous substances and methods are applied to improve the antifungal agent delivery across the nail plate. This work aimed to evaluate the effect of chemical and physical enhancers on the accumulation and permeation of amorolfine hydrochloride through human nail clippings. Polymeric nail lacquers with Eudragit E100 were developed as a potentially suitable delivery system for amorolfine hydrochloride. Incorporating thioglycolic acid and urea into formulations provided increased accumulation of antifungal agent in nail layers of up to 100% and 57%, respectively. Structural changes of nail barrier, induced by fractional CO2 laser, were visualized by microscopy. The permeation of amorolfine hydrochloride through the nail increased twofold when thioglycolic acid-containing formulation was applied and the nail was pretreated with a fractional CO2 laser. The results suggest that this novel combination of enhancers has the potential to be an effective option for topical drug delivery through the nail, and increased the efficacy of treatment.
Development of Heavyweight Self-Compacting Concrete and Ambient-Cured Heavyweight Geopolymer Concrete Using Magnetite Aggregates
Authors: Valizadeh Afsaneh , Aslani Farhad , Asif Zohaib , Roso Matt
Published: March 2019

Heavyweight self-compacting concrete (HWSCC) and heavyweight geopolymer concrete (HWGC) are new types of concrete that integrate the advantages of heavyweight concrete (HWC) with self-compacting concrete (SCC) and geopolymer concrete (GC), respectively. The replacement of natural coarse aggregates with magnetite aggregates in control SCC and control GC at volume ratios of 50%, 75%, and 100% was considered in this study to obtain heavyweight concrete classifications, according to British standards, which provide proper protection from sources that emit harmful radiations in medical and nuclear industries and may also be used in many offshore structures. The main aim of this study is to examine the fresh and mechanical properties of both types of mixes. The experimental program investigates the fresh properties of HWSCC and HWGC through the slump flow test. However, J-ring tests were only conducted for HWSCC mixes to ensure the flow requirements in order to achieve self-compacting properties. Moreover, the mechanical properties of both type of mixes were investigated after 7 and 28 days curing at an ambient temperature. The standard 100 × 200 mm cylinders were subjected to compressive and tensile tests. Furthermore, the flexural strength were examined by testing 450 × 100 × 100 mm prisms under four-point loading. The flexural load-displacement relationship for all mixes were also investigated. The results indicated that the maximum compressive strength of 53.54 MPa was achieved by using the control SCC mix after 28 days. However, in HWGC mixes, the maximum compressive strength of 31.31 MPa was achieved by 25% magnetite replacement samples. The overall result shows the strength of HWSCC decreases by increasing magnetite aggregate proportions, while, in HWGC mixes, the compressive strength increased with 50% magnetite replacement followed by a decrease in strength by 75% and 100% magnetite replacements. The maximum densities of 2901 and 2896 kg/m3 were obtained by 100% magnetite replacements in HWSCC and HWGC, respectively.
Radiation-Induced Helium Bubbles in Metals
Authors: Li Shi-Hao , Li Jing-Ting , Han Wei-Zhong
Published: March 2019

Helium (He) bubbles are typical radiation defects in structural materials in nuclear reactors after high dose energetic particle irradiation. In the past decades, extensive studies have been conducted to explore the dynamic evolution of He bubbles under various conditions and to investigate He-induced hardening and embrittlement. In this review, we summarize the current understanding of the behavior of He bubbles in metals; overview the mechanisms of He bubble nucleation, growth, and coarsening; introduce the latest methods of He control by using interfaces in nanocrystalline metals and metallic multilayers; analyze the effects of He bubbles on strength and ductility of metals; and point out some remaining questions related to He bubbles that are crucial for design of advanced radiation-tolerant materials.
Silver, Gold, and Iron Oxide Nanoparticles Alter miRNA Expression but Do Not Affect DNA Methylation in HepG2 Cells
Authors: Brzóska Kamil , Grądzka Iwona , Kruszewski Marcin
Published: March 2019

The increasing use of nanoparticles (NPs) in various applications entails the need for reliable assessment of their potential toxicity for humans. Originally, studies concerning the toxicity of NPs focused on cytotoxic and genotoxic effects, but more recently, attention has been paid to epigenetic changes induced by nanoparticles. In the present research, we analysed the DNA methylation status of genes related to inflammation and apoptosis as well as the expression of miRNAs related to these processes in response to silver (AgNPs), gold (AuNPs), and superparamagnetic iron oxide nanoparticles (SPIONs) at low cytotoxic doses in HepG2 cells. There were no significant differences between treated and control cells in the DNA methylation status. We identified nine miRNAs, the expression of which was significantly altered by treatment with nanoparticles. The highest number of changes was induced by AgNPs (six miRNAs), followed by AuNPs (four miRNAs) and SPIONs (two miRNAs). Among others, AgNPs suppressed miR-34a expression, which is of particular interest since it may be responsible for the previously observed AgNPs-mediated HepG2 cells sensitisation to tumour necrosis factor (TNF). Most of the miRNAs affected by NP treatment in the present study have been previously shown to inhibit cell proliferation and tumourigenesis. However, based on the observed changes in miRNA expression we cannot draw definite conclusions regarding the pro- or anti-tumour nature of the NPs under study. Further research is needed to fully elucidate the relation between observed changes in miRNA expression and the effect of NPs observed at the cellular level. The results of the present study support the idea of including epigenetic testing during the toxicological assessment of the biological interaction of nanomaterials.
Fatigue and Corrosion Fatigue Behaviour of Brazed Stainless Steel Joints AISI 304L/BAu-4 in Synthetic Exhaust Gas Condensate
Authors: Schmiedt-Kalenborn Anke , Lingnau Lars Andree , Manka Matthias , Tillmann Wolfgang , Walther Frank
Published: March 2019

As brazed stainless steel components in service often have to withstand cyclic loads in corrosive environments, the corrosion fatigue properties of brazed joints have to be characterised. Application-relevant corrosion fatigue tests in corrosive media are extremely rare for brazed joints and cyclic deformation curves are barely investigated. In this study, fatigue tests of brazed AISI 304L/BAu-4 joints were performed in air and synthetic exhaust gas condensate K2.2 according to VDA 230-214. The fatigue behaviour of the brazed joints was compared to properties of the austenitic base material. Strain, electrical, magnetic, temperature and electrochemical measurement techniques were applied within fatigue and corrosion fatigue tests to characterise the cyclic deformation and damage behaviour of the brazed joints. It was found that the fatigue strength of 397 MPa at 2 × 106 cycles was reduced down to 51% due to the superimposed corrosive loading. Divergent microstructure-related damage mechanisms were identified for corrosion fatigue loadings and fatigue loadings of specimens in the as-received and pre-corroded conditions. The investigations demonstrate the important role of corrosive environments for the mechanical performance of brazed stainless steel joints.
Assessment of Microstructure and Mechanical Properties of Stir Zone Seam of Friction Stir Welded Magnesium AZ31B through Nano-SiC
Authors: Subramani Vijayakumar , Jayavel Balaji , Sengottuvelu Ramesh , Lazar Prince Jeya Lal
Published: March 2019

In the present study, silicon carbide nanoparticles were incorporated into AZ31B magnesium alloy welded joints using the friction stir welding technique at five different stir zone volume fractions. The volume percentage of nano-SiC was varied from 0–20% in increments of 4%. Initially, the microstructure analyses of the V4, V8, and V12 welded joints were observed to be in good accordance with a homogeneous dispersion of nano SiC particles within the stir zone (SZ). Moreover, the particle’s agglomeration and large cluster size were found in the SZ due to insufficient heat generation of the specimen’s V16 and V20 during friction stir welding (FSW). Furthermore, the tensile and microhardness test was conducted, and the results indicate that the volume fractions increase along with the ultimate tensile strength and average microhardness, which increases up to 12% SiC addition (V12). With this effect, the fracture morphology was examined in the nano-composite joints that revealed a brittle fracture mode, which was observed in specimens V16 and V20, and the remaining was in the ductile fracture mode. From this investigation, a significant enhancement was found in the weld nugget zone that the tensile strength value of the V12 specimen was improved by 21% compared to the welded joint without SiC.
Study of a Hybrid Excitation Synchronous Machine: Modeling and Experimental Validation
Authors: Asfirane Salim , Hlioui Sami , Amara Yacine , Gabsi Mohamed
Published: March 2019

This paper deals with a parallel hybrid excitation synchronous machine (HESM). First, an expanded literature review of hybrid/double excitation machines is provided. Then, the structural topology and principles of operation of the hybrid excitation machine are examined. With the aim of validating the double excitation principle of the topology studied in this paper, the construction of a prototype is presented. In addition, both the 3D finite element method (FEM) and 3D magnetic equivalent circuit (MEC) model are used to model the machine. The flux control capability in the open-circuit condition and results of the developed models are validated by comparison with experimental measurements. The reluctance network model is created from a mesh of the studied domain. The meshing technique aims to combine advantages of finite element modeling, i.e., genericity and expert magnetic equivalent circuit models, i.e., reduced computation time. It also allows taking the non-linear characteristics of ferromagnetic materials into consideration. The machine prototype is tested to validate the predicted results. By confronting results from both modeling techniques and measurements, it is shown that the magnetic equivalent circuit model exhibits fairly accurate results when compared to the 3D finite element method with a gain in computation time.
Precise Structure and Anticoagulant Activity of Fucosylated Glycosaminoglycan from Apostichopus japonicus: Analysis of Its Depolymerized Fragments
Authors: Guan Ruowei , Peng Yuan , Zhou Lutan , Zheng Wenqi , Liu Xixi , Wang Pin , Yuan Qingxia , Gao Na , Zhao Longyan , Zhao Jinhua
Published: March 2019

Apostichopus japonicus is one of the most economically important species in sea cucumber aquaculture in China. Fucosylated glycosaminoglycan from A. japonicus (AjFG) has shown multiple pharmacological activities. However, results from studies on the structure of AjFG are still controversial. In this study, the deaminative depolymerization method that is glycosidic bond-selective was used to prepare the depolymerized products from AjFG (dAjFG), and then a series of purified oligosaccharide fragments such as tri-, hexa-, nona-, and dodecasaccharides were obtained from dAjFG by gel permeation chromatography. The 1D/2D NMR and ESI-MS spectrometry analyses showed that these oligosaccharides had the structural formula of l-FucS-α1,3-d-GlcA-β1,3-{d-GalNAc4S6S-β1,4-[l-FucS-α1,3-]d-GlcA-β1,3-}n-d-anTal-diol4S6S (n = 0, 1, 2, 3; FucS represents Fuc2S4S, Fuc3S4S, or Fuc4S). Thus, the unambiguous structure of native AjFG can be rationally deduced: it had the backbone of {-4-d-GlcA-β1,3-d-GalNAc4S6S-β1-}n, which is similar to chondroitin sulfate E, and each d-GlcA residue in the backbone was branched with a l-FucS monosaccharide at O-3. Bioactivity assays confirmed that dAjFG and nonasaccharides and dodecasaccharides from AjFG had potent anticoagulant activity by intrinsic FXase inhibition while avoiding side effects such as FXII activation and platelet aggregation.
Identification of a Recombinant Human Interleukin-12 (rhIL-12) Fragment in Non-Reduced SDS-PAGE
Authors: Yu Lei , Li Yonghong , Tao Lei , Jia Chuncui , Yao Wenrong , Rao Chunming , Wang Junzhi
Published: March 2019

During the past two decades, recombinant human interleukin-12 (rhIL-12) has emerged as one of the most potent cytokines in mediating antitumor activity in a variety of preclinical models and clinical studies. Purity is a critical quality attribute (CQA) in the quality control system of rhIL-12. In our study, rhIL-12 bulks from manufacturer B showed a different pattern in non-reduced SDS-PAGE compared with size-exclusion chromatography (SEC)-HPLC. A small fragment was only detected in non-reduced SDS-PAGE but not in SEC-HPLC. The results of UPLC/MS and N-terminal sequencing confirmed that the small fragment was a 261–306 amino acid sequence of a p40 subunit of IL-12. The cleavage occurs between Lys260 and Arg261, a basic rich region. With the presence of 0.2% SDS, the small fragment appeared in both native PAGE and in SEC-HPLC, suggesting that it is bound to the remaining part of the IL-12 non-covalently, and is dissociated in a denatured environment. The results of a bioassay showed that the fractured rhIL-12 proteins had deficient biological activity. These findings provide an important reference for the quality control of the production process and the final products of rhIL-12.
Synthetic Peptide Purification via Solid-Phase Extraction with Gradient Elution: A Simple, Economical, Fast, and Efficient Methodology
Authors: Insuasty Cepeda Diego Sebastián , Pineda Castañeda Héctor Manuel , Rodríguez Mayor Andrea Verónica , García Castañeda Javier Eduardo , Maldonado Villamil Mauricio , Fierro Medina Ricardo , Rivera Monroy Zuly Jenny
Published: March 2019

A methodology was implemented for purifying peptides in one chromatographic run via solid-phase extraction (SPE), reverse phase mode (RP), and gradient elution, obtaining high-purity products with good yields. Crude peptides were analyzed by reverse phase high performance liquid chromatography and a new mathematical model based on its retention time was developed in order to predict the percentage of organic modifier in which the peptide will elute in RP-SPE. This information was used for designing the elution program of each molecule. It was possible to purify peptides with different physicochemical properties, showing that this method is versatile and requires low solvent consumption, making it the least polluting one. Reverse phase-SPE can easily be routinely implemented. It is an alternative to enrich and purified synthetic or natural molecules.
Smoke-Isolated Karrikins Stimulated Tanshinones Biosynthesis in Salvia miltiorrhiza through Endogenous Nitric Oxide and Jasmonic Acid
Authors: Zhou Jie , Xu Zi-xin , Sun Hui , Guo Lan-ping
Published: March 2019

Although smoke-isolated karrikins (KAR1) could regulate secondary metabolism in medicinal plants, the signal transduction mechanism has not been reported. This study highlights the influence of KAR1 on tanshinone I (T-I) production in Salvia miltiorrhiza and the involved signal molecules. Results showed KAR1-induced generation of nitric oxide (NO), jasmonic acid (JA) and T-I in S. miltiorrhiza hairy root. KAR1-induced increase of T-I was suppressed by NO-specific scavenger (cPTIO) and NOS inhibitors (PBITU); JA synthesis inhibitor (SHAM) and JA synthesis inhibitor (PrGall), which indicated that NO and JA play essential roles in KAR1-induced T-I. NO inhibitors inhibited KAR1-induced generation of NO and JA, suggesting NO was located upstream of JA signal pathway. NO-induced T-I production was inhibited by SHAM and PrGall, implying JA participated in transmitting signal NO to T-I accumulation. In other words, NO mediated the KAR1-induced T-I production through a JA-dependent signaling pathway. The results helped us understand the signal transduction mechanism involved in KAR1-induced T-I production and provided helpful information for the production of S. miltiorrhiza hairy root.
Concerted Catalysis by Nanocellulose and Proline in Organocatalytic Michael Additions
Authors: Ranaivoarimanana Naliharifetra Jessica , Kanomata Kyohei , Kitaoka Takuya
Published: March 2019

Cellulose nanofibers (CNFs) have recently attracted much attention as catalysts in various reactions. Organocatalysts have emerged as sustainable alternatives to metal-based catalysts in green organic synthesis, with concerted systems containing CNFs that are expected to provide next-generation catalysis. Herein, for the first time, we report that a representative organocatalyst comprising an unexpected combination of 2,2,6,6-tetramethylpiperidine 1-oxyl (TEMPO)-oxidized CNFs and proline shows significantly enhanced catalytic activity in an asymmetric Michael addition.
An Efficient, One-Pot Transamidation of 8-Aminoquinoline Amides Activated by Tertiary-Butyloxycarbonyl
Authors: Wu Wengang , Yi Jun , Xu Huipeng , Li Shuangjun , Yuan Rongxin
Published: March 2019

The efficient, one-pot access to the transamidation of 8-aminoquinoline (8-AQ), notorious for its harsh removal conditions, has been widely employed as an auxiliary in C–H functionalization reactions due to its strong directing ability. In this study, the facile and mild Boc protection of the corresponding 8-AQ amide was critical to activate the amide C(acyl)–N bond by twisting its geometry to lower the amidic resonance energy. Both aryl and alkyl amines proceeded transamidation in one-pot, user-friendly conditions with excellent yields.
The Reform of School Catering in Hungary: Anatomy of a Health-Education Attempt
Authors: Kiss Anna , Popp József , Oláh Judit , Lakner Zoltán
Published: March 2019

School lunch nutrition standards are an important carrier of messages on healthy eating and an efficient way of changing the nutritional behaviour of new generations. Many countries in Europe have a compulsory system of school meals; the Hungarian government also wanted to take action in order to improve the nutrition requirements of the school catering service. The Hungarian Ministry of Human Resources established some limits in the school catering system. However, increasing public pressure forced the legislating organ to considerably modify this regulation. The aim of this study is to analyse the causes of this failure, based on a conceptual framework of institutional economics and a strategic modelling of different institutes by examining the results of 72 interviews (33 experts, 26 parents and 13 teachers) conducted with representatives of different stakeholders. The results highlight the lack of preparation for the introduction of the new regulatory framework, as well as the inefficient communication between the different stakeholders. In order to support children in eating healthfully, a complex nutrition education program and continuous dialogue is needed between teachers, parents, catering staff and the government.
Lipid Accumulation and Chronic Kidney Disease
Authors: Gai Zhibo , Wang Tianqi , Visentin Michele , Kullak-Ublick Gerd , Fu Xianjun , Wang Zhenguo
Published: March 2019

Obesity and hyperlipidemia are the most prevalent independent risk factors of chronic kidney disease (CKD), suggesting that lipid accumulation in the renal parenchyma is detrimental to renal function. Non-esterified fatty acids (also known as free fatty acids, FFA) are especially harmful to the kidneys. A concerted, increased FFA uptake due to high fat diets, overexpression of fatty acid uptake systems such as the CD36 scavenger receptor and the fatty acid transport proteins, and a reduced β-oxidation rate underlie the intracellular lipid accumulation in non-adipose tissues. FFAs in excess can damage podocytes, proximal tubular epithelial cells and the tubulointerstitial tissue through various mechanisms, in particular by boosting the production of reactive oxygen species (ROS) and lipid peroxidation, promoting mitochondrial damage and tissue inflammation, which result in glomerular and tubular lesions. Not all lipids are bad for the kidneys: polyunsaturated fatty acids (PUFA) such as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) seem to help lag the progression of chronic kidney disease (CKD). Lifestyle interventions, especially dietary adjustments, and lipid-lowering drugs can contribute to improve the clinical outcome of patients with CKD.
The Effects of Dietary Supplements on Asthma and Lung Cancer Risk in Smokers and Non-Smokers: A Review of the Literature
Authors: Alsharairi Naser A.
Published: March 2019

Smoking is one of the major global causes of death. Cigarette smoke and secondhand (passive) smoke have been causally related to asthma and lung cancer. Asthma is a potential risk factor for developing lung cancer in both smokers and non-smokers. Prospective studies and randomized control trials (RCTs) of dietary supplements and lung cancer risk in adult smokers and non-smokers have yielded inconsistent results. A few prospective studies have shown that long-term use of high doses of some supplements, such as retinol, β-carotene, B vitamins, and vitamin E, increase lung cancer risk in current and former smokers. Limited evidence from RCTs suggests that vitamin D supplementation is effective in improving lung function and reducing asthma risk in current/former smokers. The relationship between dietary supplements and lung cancer risk has never before been examined in asthmatic smokers and non-smokers. This short review aims to examine the evidence from existing studies for the effects of dietary supplements on asthma/lung cancer risk and mortality in smokers and non-smokers.
Gravitational Waves from Mirror World
Authors: Beradze Revaz , Gogberashvili Merab
Published: March 2019

In this paper we consider the properties of the 10 confirmed by the LIGO (Laser Interferometer Gravitational-Wave Observatory) Collaboration gravitational wave signals from the black hole mergers. We want to explain non-observation of electromagnetic counterpart and higher then expected merging rates of these events, assuming the existence of their sources in the hidden mirror universe. Mirror matter, which interacts with our world only through gravity, is a candidate of dark matter and its density can exceed ordinary matter density five times. Since mirror world is considered to be colder, star formation there started earlier and mirror black holes had more time to pick up the mass and to create more binary systems within the LIGO reachable zone. In total, we estimate factor of 15 amplification of black holes merging rate in mirror world with respect to our world, which is consistent with the LIGO observations.
Divine Light and Melodies Lead the Way: The Santmat Tradition of Bihar
Authors: Howard Veena R.
Published: March 2019

This paper focuses on the branch of Santmat (thus far, unstudied by scholars of Indian religions), prevalent in the rural areas of Bihar, India. Santmat—literally meaning “the Path of Sants” or “Point of View of the Sants”—of Bihar represents a unique synthesis of the elements of the Vedic traditions, rural Hindu practices, and esoteric experiences, as recorded in the poetry of the medieval Sant Tradition. I characterize this tradition as “Santmat of Bihar” to differentiate it from the other branches of Santmat. The tradition has spread to all parts of India, but its highest concentration remains in Bihar. Maharishi Mehi, a twentieth-century Sant from Bihar State, identifies Santmat’s goal as śānti. Maharishi Mehi defines Śānti as the state of deep stillness, equilibrium, and the unity with the Divine. He considers those individuals sants who are established in this state. The state of sublime peace is equally available to all human beings, irrespective of gender, religion, ethnicity, or status. However, it requires a systematic path. Drawing on the writings of the texts of Sanātana Dharma, teachings of the Sants and personal experiences, Maharishi Mehi lays out a systematic path that encompasses the moral observances and detailed esoteric experiences. He also provides an in-depth description of the esoteric practices of divine light (dṛṣti yoga) and sound (surat śabda yoga) in the inner meditation. After providing a brief overview of the history and distinctive features of Santmat of Bihar, this paper will focus on the specifics and unique interpretations of the four structural principles of the tradition: Guru (spiritual teacher), dhyān (inner path of mediation), satsaṅg (spiritual discourses or congregating practitioners for meditation or study), and sadācār (moral conduct). Through a close analysis of textual sources, Sants’ oral discourses that I translated, as well as insights from my participant-observant experiences, I will examine how the four elements reorient the practitioner from the mundane world to the sacred inner experience of śānti.