bluuebunny/crossref_metadata_2025 huggingface dataset:
Natural Pest Regulation and Its Compatibility with Other Crop Protection Practices in Smallholder Bean Farming Systems
Authors: Ndakidemi Baltazar J. , Mbega Ernest R. , Ndakidemi Patrick A. , Stevenson Philip C. , Belmain Steven R. , Arnold Sarah E. J. , Woolley Victoria C.
Published: August 2021

Common bean (Phaseolus vulgaris) production and storage are limited by numerous constraints. Insect pests are often the most destructive. However, resource-constrained smallholders in sub-Saharan Africa (SSA) often do little to manage pests. Where farmers do use a control strategy, it typically relies on chemical pesticides, which have adverse effects on the wildlife, crop pollinators, natural enemies, mammals, and the development of resistance by pests. Nature-based solutions —in particular, using biological control agents with sustainable approaches that include biopesticides, resistant varieties, and cultural tools—are alternatives to chemical control. However, significant barriers to their adoption in SSA include a lack of field data and knowledge on the natural enemies of pests, safety, efficacy, the spectrum of activities, the availability and costs of biopesticides, the lack of sources of resistance for different cultivars, and spatial and temporal inconsistencies for cultural methods. Here, we critically review the control options for bean pests, particularly the black bean aphid (Aphis fabae) and pod borers (Maruca vitrata). We identified natural pest regulation as the option with the greatest potential for this farming system. We recommend that farmers adapt to using biological control due to its compatibility with other sustainable approaches, such as cultural tools, resistant varieties, and biopesticides for effective management, especially in SSA.
SnoRNA in Cancer Progression, Metastasis and Immunotherapy Response
Authors: van der Werf Jildou , Chin Chue , Fleming Nicholas
Published: August 2021

Small nucleolar RNA (snoRNA) were one of our earliest recognised classes of non-coding RNA, but were largely ignored by cancer investigators due to an assumption that their activities were confined to the nucleolus. However, as full genome sequences have become available, many new snoRNA genes have been identified, and multiple studies have shown their functions to be diverse. The consensus now is that many snoRNA are dysregulated in cancers, are differentially expressed between cancer types, stages and metastases, and they can actively modify disease progression. In addition, the regulation of the snoRNA class is dominated by the cancer-supporting mTOR signalling pathway, and they may have particular significance to immune cell function and anti-tumour immune responses. Given the recent advent of therapeutics that can target RNA molecules, snoRNA have robust potential as drug targets, either solely or in the context of immunotherapies.
Effects of Epoxyeicosatrienoic Acid-Enhancing Therapy on the Course of Congestive Heart Failure in Angiotensin II-Dependent Rat Hypertension: From mRNA Analysis towards Functional In Vivo Evaluation
Authors: Kala Petr , Miklovič Matúš , Jíchová Šárka , Škaroupková Petra , Vaňourková Zdeňka , Maxová Hana , Gawrys Olga , Kompanowska-Jezierska Elzbieta , Sadowski Janusz , Imig John D. , Falck John R. , Veselka Josef , Červenka Luděk , Aiglová Renáta , Vícha Marek , Gloger Vít , Táborský Miloš
Published: August 2021

This study evaluates the effects of chronic treatment with EET-A, an orally active epoxyeicosatrienoic acid (EETs) analog, on the course of aorto-caval fistula (ACF)-induced heart failure (HF) in Ren-2 transgenic rats (TGR), a model characterized by hypertension and augmented activity of the renin-angiotensin system (RAS). The results were compared with standard pharmacological blockade of the RAS using angiotensin-converting enzyme inhibitor (ACEi). The rationale for employing EET-A as a new treatment approach is based on our findings that apart from increased RAS activity, untreated ACF TGR also shows kidney and left ventricle (LV) tissue deficiency of EETs. Untreated ACF TGR began to die 17 days after creating ACF and were all dead by day 84. The treatment with EET-A alone or ACEi alone improved the survival rate: in 156 days after ACF creation, it was 45.5% and 59.4%, respectively. The combined treatment with EET-A and ACEi appeared to improve the final survival to 71%; however, the difference from either single treatment regimen did not reach significance. Nevertheless, our findings support the notion that targeting the cytochrome P-450-dependent epoxygenase pathway of arachidonic acid metabolism should be considered for the treatment of HF.
The Histone Variant MacroH2A1 Impacts Circadian Gene Expression and Cell Phenotype in an In Vitro Model of Hepatocellular Carcinoma
Authors: Carbone Annalucia , De Santis Elisabetta , Cela Olga , Giambra Vincenzo , Miele Luca , Marrone Giuseppe , Grieco Antonio , Buschbeck Marcus , Capitanio Nazzareno , Mazza Tommaso , Mazzoccoli Gianluigi
Published: August 2021

Hepatocellular carcinoma (HCC) is a leading cause of cancer-related death worldwide. A foremost risk factor for HCC is obesity/metabolic syndrome-related non-alcoholic fatty liver disease (NAFLD) and non-alcoholic steatohepatitis (NASH), which is prompted by remarkable changes in transcription patterns of genes enriching metabolic, immune/inflammatory, and circadian pathways. Epigenetic mechanisms play a role in NAFLD-associated HCC, and macroH2A1, a variant of histone H2A, is involved in the pathogenesis modulating the expression of oncogenes and/or tumor suppressor genes and interacting with SIRT1, which crucially impacts the circadian clock circuitry. Hence, we aimed to appraise if and how macroH2A1 regulated the expression patterns of circadian genes in the setting of NAFLD-associated HCC. We took advantage of an in vitro model of liver cancer represented by HepG2 (human hepatocarcinoma) cells stably knocked down for macroH2A1 and conducted whole transcriptome profiling and deep phenotyping analysis. We found up-regulation of PER1 along with several deregulated circadian genes, enriching several important pathways and functions related to cancer onset and progression, such as epithelial-to-mesenchymal transition, cell cycle deregulation, and DNA damage. PER1 silencing partially mitigated the malignant phenotype induced by the loss of macroH2A1 in HCC cells. In conclusion, our findings suggest a modulatory role for the core circadian protein PER1 in liver carcinogenesis in the context of a lack of the macroH2A1 epigenetic and transcriptional landscape.
Proteomic Analysis Reveals Key Proteins in Extracellular Vesicles Cargo Associated with Idiopathic Pulmonary Fibrosis In Vitro
Authors: Velázquez-Enríquez Juan Manuel , Santos-Álvarez Jovito Cesar , Ramírez-Hernández Alma Aurora , Reyes-Jiménez Edilburga , López-Martínez Armando , Pina-Canseco Socorro , Aguilar-Ruiz Sergio Roberto , Romero-Tlalolini María de los Ángeles , Castro-Sánchez Luis , Arellanes-Robledo Jaime , Vásquez-Garzón Verónica Rocío , Baltiérrez-Hoyos Rafael
Published: August 2021

Idiopathic pulmonary fibrosis (IPF) is a chronic, progressive, irreversible, and highly fatal disease. It is characterized by the increased activation of both fibroblast and myofibroblast that results in excessive extracellular matrix (ECM) deposition. Extracellular vesicles (EVs) have been described as key mediators of intercellular communication in various pathologies. However, the role of EVs in the development of IPF remains poorly understood. This study aimed to characterize the differentially expressed proteins contained within EVs cargo derived from the fibroblast cell lines LL97A (IPF-1) and LL29 (IPF-2) isolated from lungs bearing IPF as compared to those derived from the fibroblast cell lines CCD8Lu (NL-1) and CCD19Lu (NL-2) isolated from healthy donors. Isolated EVs were subjected to label-free quantitative proteomic analysis by LC-MS/MS, and as a result, 331 proteins were identified. Differentially expressed proteins were obtained after the pairwise comparison, including all experimental groups. A total of 86 differentially expressed proteins were identified in either one or more comparison groups. Of note, proteins involved in fibrogenic processes, such as tenascin-c (TNC), insulin-like-growth-factor-binding protein 7 (IGFBP7), fibrillin-1 (FBN1), alpha-2 collagen chain (I) (COL1A2), alpha-1 collagen chain (I) (COL1A1), and lysyl oxidase homolog 1 (LOXL1), were identified in EVs cargo isolated from IPF cell lines. Additionally, KEGG pathway enrichment analysis revealed that differentially expressed proteins participate in focal adhesion, PI3K-Akt, and ECM–receptor interaction signaling pathways. In conclusion, our findings reveal that proteins contained within EVs cargo might play key roles during IPF pathogenesis.
Mirror Visual Feedback Induces M1 Excitability by Disengaging Functional Connections of Perceptuo-Motor-Attentional Processes during Asynchronous Bimanual Movement: A Magnetoencephalographic Study
Authors: Lin Szu-Hung , Cheng Chia-Hsiung , Wu Ching-Yi , Liu Chien-Ting , Chen Chia-Ling , Hsieh Yu-Wei
Published: August 2021

Mirror visual feedback (MVF) has been shown to increase the excitability of the primary motor cortex (M1) during asynchronous bimanual movement. However, the functional networks underlying this process remain unclear. We recruited 16 healthy volunteers to perform asynchronous bimanual movement, that is, their left hand performed partial range of movement while their right hand performed normal full range of movement. Their ongoing brain activities were recorded by whole-head magnetoencephalography during the movement. Participants were required to keep both hands stationary in the control condition. In the other two conditions, participants were required to perform asynchronous bimanual movement with MVF (Asy_M) and without MVF (Asy_w/oM). Greater M1 excitability was found under Asy_M than under Asy_w/oM. More importantly, when receiving MVF, the visual cortex reduced its functional connection to brain regions associated with perceptuo-motor-attentional process (i.e., M1, superior temporal gyrus, and dorsolateral prefrontal cortex). This is the first study to demonstrate a global functional network of MVF during asynchronous bimanual movement, providing a foundation for future research to examine the neural mechanisms of mirror illusion in motor control.
Noninvasive Optical Monitoring of Cerebral Blood Flow and EEG Spectral Responses after Severe Traumatic Brain Injury: A Case Report
Authors: Poon Chien-Sing , Rinehart Benjamin , Langri Dharminder S. , Rambo Timothy M. , Miller Aaron J. , Foreman Brandon , Sunar Ulas
Published: August 2021

Survivors of severe brain injury may require care in a neurointensive care unit (neuro-ICU), where the brain is vulnerable to secondary brain injury. Thus, there is a need for noninvasive, bedside, continuous cerebral blood flow monitoring approaches in the neuro-ICU. Our goal is to address this need through combined measurements of EEG and functional optical spectroscopy (EEG-Optical) instrumentation and analysis to provide a complementary fusion of data about brain activity and function. We utilized the diffuse correlation spectroscopy method for assessing cerebral blood flow at the neuro-ICU in a patient with traumatic brain injury. The present case demonstrates the feasibility of continuous recording of noninvasive cerebral blood flow transients that correlated well with the gold-standard invasive measurements and with the frequency content changes in the EEG data.
Memantine Protects against Paclitaxel-Induced Cognitive Impairment through Modulation of Neurogenesis and Inflammation in Mice
Authors: Sung Pi-Shan , Chen Pei-Wen , Yen Chia-Jui , Shen Meng-Ru , Chen Chih-Hung , Tsai Kuen-Jer , Lin Chou-Ching K.
Published: August 2021

Chemotherapy-induced cognitive impairment (CICI) is an adverse side effect of cancer treatment with increasing awareness. Hippocampal damage and related neurocognitive impairment may mediate the development of CICI, in which altered neurogenesis may play a role. In addition, increased inflammation may be related to chemotherapy-induced hippocampal damage. Memantine, an uncompetitive N-methyl-D-aspartate (NMDA) receptor antagonist that may enhance neurogenesis and modulate inflammation, may be useful for treating CICI. To test this hypothesis, paclitaxel was administered to eight-week-old male B6 mice to demonstrate the relationship between CICI and impaired neurogenesis, and then, we evaluated the impact of different memantine regimens on neurogenesis and inflammation in this CICI model. The results demonstrated that both the pretreatment and cotreatment regimens with memantine successfully reversed impaired neurogenesis and spatial memory impairment in behavior tests. The pretreatment regimen unsuccessfully inhibited the expression of peripheral and central TNF-α and IL-1β and did not improve the mood alterations following paclitaxel treatment. However, the cotreatment regimen led to a better modulatory effect on inflammation and restoration of mood disturbance. In conclusion, this study illustrated that impaired neurogenesis is one of the mechanisms of paclitaxel-induced CICI. Memantine may serve as a potential treatment for paclitaxel-induced CICI, but different treatment strategies may lead to variations in the treatment efficacy.
Glucose Transporters as a Target for Anticancer Therapy
Authors: Pliszka Monika , Szablewski Leszek
Published: August 2021

Tumor growth causes cancer cells to become hypoxic. A hypoxic condition is a hallmark of cancer. Metabolism of cancer cells differs from metabolism of normal cells. Cancer cells prefer the process of glycolysis as a source of ATP. Process of glycolysis generates only two molecules of ATP per one molecule of glucose, whereas the complete oxidative breakdown of one molecule of glucose yields 36 molecules of ATP. Therefore, cancer cells need more molecules of glucose in comparison with normal cells. Increased uptake of glucose by these cells is due to overexpression of glucose transporters, especially GLUT1 and GLUT3, that are hypoxia responsive, as well as other glucose transport proteins. Increased expression of these carrier proteins may be used in anticancer therapy. This phenomenon is used in diagnostic techniques such as FDG-PET. It is also suggested, and there are observations, that therapeutic inhibition of glucose transporters may be a method in treatment of cancer patients. On the other hand, there are described cases, in which upregulation of glucose transporters, as, for example, NIS, which is used in radioiodine therapy, can help patients with cancer. The aim of this review is the presentation of possibilities, and how glucose transporters can be used in anticancer therapy.
Unraveling Tumor Heterogeneity by Using DNA Barcoding Technologies to Develop Personalized Treatment Strategies in Advanced-Stage PDAC
Authors: Dujardin Philip , Baginska Anna K. , Urban Sebastian , Grüner Barbara M.
Published: August 2021

Tumor heterogeneity is a hallmark of many solid tumors, including pancreatic ductal adenocarcinoma (PDAC), and an inherent consequence of the clonal evolution of cancers. As such, it is considered the underlying concept of many characteristics of the disease, including the ability to metastasize, adapt to different microenvironments, and to develop therapy resistance. Undoubtedly, the high mortality of PDAC can be attributed to a high extent to these properties. Despite its apparent importance, studying tumor heterogeneity has been a challenging task, mainly due to its complexity and lack of appropriate methods. However, in recent years molecular DNA barcoding has emerged as a sophisticated tool that allows mapping of individual cells or subpopulations in a cell pool to study heterogeneity and thus devise new personalized treatment strategies. In this review, we provide an overview of genetic and non-genetic inter- and intra-tumor heterogeneity and its impact on (personalized) treatment strategies in PDAC and address how DNA barcoding technologies work and can be applied to study this clinically highly relevant question.
A Transcriptomic Approach Reveals Selective Ribosomal Remodelling in the Tumour Versus the Stromal Compartment of Metastatic Colorectal Cancer
Authors: Lastraioli Elena , Ruffinatti Federico Alessandro , Di Costanzo Francesco , Sala Cesare , Munaron Luca , Arcangeli Annarosa
Published: August 2021

Because of its high incidence and poor prognosis, colorectal cancer (CRC) represents an important health issue in several countries. As with other carcinomas, the so-called tumour microenvironment (TME) has been shown to play key roles in CRC progression and related therapeutical outcomes, even though a deeper understanding of the underlying molecular mechanisms is needed to devise new treatment strategies. For some years now, omics technologies and consolidated bioinformatics pipelines have allowed scientists to access large amounts of biologically relevant information, even when starting from small tissue samples; thus, in order to shed new light upon the role of the TME in CRC, we compared the gene expression profiles of 6 independent tumour tissues (all progressed towards metastatic disease) to the expression profile of the surrounding stromata. To do this, paraffin-embedded whole tissues were first microdissected to obtain samples enriched with tumour and stromal cells, respectively. Afterwards, RNA was extracted and analysed using a microarray-based approach. A thorough bioinformatics analysis was then carried out to identify transcripts differentially expressed between the two groups and possibly enriched functional terms. Overall, 193 genes were found to be significantly downregulated in tumours compared to the paired stromata. The functional analysis of the downregulated gene list revealed three principal macro areas of interest: the extracellular matrix, cell migration, and angiogenesis. Conversely, among the upregulated genes, the main alterations detected by the functional annotation were related to the ribosomal proteins (rProteins) of both the large (60S) and small (40S) subunits of the cytosolic ribosomes. Subsequent gene set enrichment analysis (GSEA) confirmed the massive overexpression of most cytosolic—but not mitochondrial—ribosome rProteins.
Efficacy of the mRNA-Based BNT162b2 COVID-19 Vaccine in Patients with Solid Malignancies Treated with Anti-Neoplastic Drugs
Authors: Agbarya Abed , Sarel Ina , Ziv-Baran Tomer , Agranat Sivan , Schwartz Orna , Shai Ayelet , Nordheimer Sharon , Fenig Shlomit , Shechtman Yelena , Kozlener Ella , Taha Tarek , Nasrallah Haitam , Parikh Roma , Elkoshi Nadav , Levy Carmit , Khoury Rasha , Brenner Ronen
Published: August 2021

The BNT162b2 vaccine was shown to be highly effective in reducing the risk of COVID-19 infection in healthy individuals and patients with chronic disease. However, there are little data regarding its efficacy in patients treated for cancer. We analyzed the humoral response following vaccination with the second dose of BNT162b2 in 140 patients with solid malignancies who were receiving anti-cancer therapy at the time of vaccination and 215 participants who had not been diagnosed with cancer. Multivariate analysis was performed, followed by matching the two groups by age, gender and days from vaccination. The humoral response in the cancer patient group was significantly lower than in the non-cancer group: 20/140 seronegative (14.3%) vs. 3/215 (1.4%), p < 0.001; median IgG levels 2231 AU/mL (IQR 445-8023) vs. 4100 (IQR 2231-6774) p = 0.001 respectively. The odds ratio for negative serology results in cancer patients adjusted by age and gender was 7.35 compared to participants without cancer. This effect was observed only in chemotherapy treated patients: 17/73 seronegative (23.3%) vs. 3/215 (1.4%), p < 0.001; median IgG 1361 AU/mL vs. 4100, p < 0.001 but not in patients treated with non-chemotherapeutic drugs. Reduced immunogenicity to COVID-19 vaccine among chemotherapy-treated cancer patients, raises the need to continue exercising protective measures after vaccination in these patients.
Outcome of 177Lu-PSMA-617 Radioligand Therapy in Chemo-Refractory Patients with Metastatic Castration-Resistant Early-Onset Prostate Cancer
Authors: Mader Nicolai , Groener Daniel , Tselis Nikolaos , Banek Séverine , Nagarajah James , Grünwald Frank , Sabet Amir
Published: August 2021

The aim of this retrospective study was to assess the outcome of patients with metastasized castration-resistant early-onset prostate cancer refractory to chemotherapy receiving radioligand therapy with 177Lutetium-PSMA-617 (LuPSMA-RLT). Twenty-five patients of ≤55 years of age at prostate cancer diagnosis, treated with a median of four (IQR 2–6) cycles (mean of 7.7 ± 1.4 GBq per cycle) every 6–8 weeks, were analyzed. Survival outcome was calculated based on the Kaplan–Meier method. The median progression-free survival (PFS) was 3.8 months (95% CI 2.3–5.3), and overall survival (OS) was 8.5 months (95% CI 6.2–10.8). An initial PSA reduction (≥ 50%) was observed in 9/25 (36%) of patients without being significantly associated with OS (p = 0.601). PSA response (PSA decline ≥50% at 12 weeks) was observed in 12/25 (48%) of patients and significantly associated with longer OS (16.0 months, 95% CI 7.4–24.6 vs. 4.0 months, 95% CI 1.1–6.9, p = 0.002). Imaging-based response using 68Ga-PSMA-11-PET/CT after two to three cycles was seen in 11/25 (44%). Additionally, responders had a significantly longer median PFS (8.7 months, 95% CI 1.3–16.1 vs. 1.9 months, 95% CI 1.7–2.2, p < 0.001) and OS (16.0 months, 95% CI 7.6–24.4 vs. 4.0 months, 95% CI 0.9–7.1; p = 0.002). Intra- or post-therapeutic toxicity was graded according to the CTCAE v5.0 criteria. Newly developing grade ≥ 3 anemia, leukopenia, and thrombocytopenia occurred in three (12%), one (4%), and three (12%) patients, respectively. One patient showed renal toxicity (grade ≥ 3) during follow-up. Pain palliation (>2 level VAS decline) was achieved in 9/14 (64%) and performance status improvement (ECOG level decline ≥ 1) in 8/17 (47%) of patients. Compared to previous reports, radioligand therapy with 177Lu-PSMA-617 in metastasized castration-resistant early-onset prostate cancer patients refractory to chemotherapy yields similar response rates with a comparable safety profile, but is associated with shorter survival.
Germline PALB2 Mutation in High-Risk Chinese Breast and/or Ovarian Cancer Patients
Authors: Kwong Ava , Shin Vivian Y. , Ho Cecilia Y. S. , Khalid Aleena , Au Chun Hang , Chan Karen K. L. , Ngan Hextan Y. S. , Chan Tsun-Leung , Ma Edmond S. K.
Published: August 2021

The prevalence of the PALB2 mutation in breast cancer varies across different ethnic groups; hence, it is of intense interest to evaluate the cancer risk and clinical association of the PALB2 mutation in Chinese breast and/or ovarian cancer patients. We performed sequencing with a 6-gene test panel (BRCA1, BRCA2, TP53, PTEN, PALB2, and CDH1) to identify the prevalence of the PALB2 germline mutation among 2631 patients with breast and/or ovarian cancer. In this cohort, 39 mutations were identified with 24 types of mutation variants, where the majority of the mutations were frame-shift mutations and resulted in early termination. We also identified seven novel PALB2 mutations. Most of the PALB2 mutation carriers had breast cancer (36, 92.3%) and were more likely to have family history of breast cancer (19, 48.7%). The majority of the breast tumors were invasive ductal carcinoma (NOS type) (34, 81.0%) and hormonal positive (ER: 32, 84.2%; PR: 23, 60.5%). Pathogenic mutations of PALB2 were found in 39 probands with a mutation frequency of 1.6% and 1% in breast cancer and ovarian cancer patients, respectively. PALB2 mutation carriers were more likely have hormonal positive tumors and were likely to have familial aggregation of breast cancer.
Accelerating the Design of Photocatalytic Surfaces for Antimicrobial Application: Machine Learning Based on a Sparse Dataset
Authors: Park Heesoo , Bentria El Tayeb , Rtimi Sami , Arredouani Abdelilah , Bensmail Halima , El-Mellouhi Fedwa
Published: August 2021

Nowadays, most experiments to synthesize and test photocatalytic antimicrobial materials are based on trial and error. More often than not, the mechanism of action of the antimicrobial activity is unknown for a large spectrum of microorganisms. Here, we propose a scheme to speed up the design and optimization of photocatalytic antimicrobial surfaces tailored to give a balanced production of reactive oxygen species (ROS) upon illumination. Using an experiment-to-machine-learning scheme applied to a limited experimental dataset, we built a model that can predict the photocatalytic activity of materials for antimicrobial applications over a wide range of material compositions. This machine-learning-assisted strategy offers the opportunity to reduce the cost, labor, time, and precursors consumed during experiments that are based on trial and error. Our strategy may significantly accelerate the large-scale deployment of photocatalysts as a promising route to mitigate fomite transmission of pathogens (bacteria, viruses, fungi) in hospital settings and public places.
Sustainable One-Pot Immobilization of Enzymes in/on Metal-Organic Framework Materials
Authors: Molina M. Asunción , Gascón-Pérez Victoria , Sánchez-Sánchez Manuel , Blanco Rosa M.
Published: August 2021

The industrial use of enzymes generally necessitates their immobilization onto solid supports. The well-known high affinity of enzymes for metal-organic framework (MOF) materials, together with the great versatility of MOFs in terms of structure, composition, functionalization and synthetic approaches, has led the scientific community to develop very different strategies for the immobilization of enzymes in/on MOFs. This review focuses on one of these strategies, namely, the one-pot enzyme immobilization within sustainable MOFs, which is particularly enticing as the resultant biocomposite Enzyme@MOFs have the potential to be: (i) prepared in situ, that is, in just one step; (ii) may be synthesized under sustainable conditions: with water as the sole solvent at room temperature with moderate pHs, etc.; (iii) are able to retain high enzyme loading; (iv) have negligible protein leaching; and (v) give enzymatic activities approaching that given by the corresponding free enzymes. Moreover, this methodology seems to be near-universal, as success has been achieved with different MOFs, with different enzymes and for different applications. So far, the metal ions forming the MOF materials have been chosen according to their low price, low toxicity and, of course, their possibility for generating MOFs at room temperature in water, in order to close the cycle of economic, environmental and energy sustainability in the synthesis, application and disposal life cycle.
Organocatalysis for the Asymmetric Michael Addition of Cycloketones and α, β-Unsaturated Nitroalkenes
Authors: Shim Jae Ho , Ahn Byung Kook , Lee Ji Yeon , Kim Hyeon Soo , Ha Deok-Chan
Published: August 2021

Michael addition is one of the most important carbon–carbon bond formation reactions. In this study, an (R, R)-1,2-diphenylethylenediamine (DPEN)-based thiourea organocatalyst was applied to the asymmetric Michael addition of nitroalkenes and cycloketones to produce a chiral product. The primary amine moiety in DPEN reacts with the ketone to form an enamine and is activated through the hydrogen bond formation between the nitro group in the α, β-unsaturated nitroalkene and thiourea. Here, the aim was to obtain an asymmetric Michael product through the 1,4-addition of the enamine to an alkene to form a new carbon–carbon bond. As a result, the primary amine of the chiral diamine was converted into an enamine. The reaction proceeded with a relatively high level of enantioselectivity achieved using double activation through the hydrogen bonding of the nitro group and thiourea. Michael products with high levels of enantioselectivity (76–99% syn ee) and diastereoselectivity (syn/anti = 9/1) were obtained with yields in the range of 88–99% depending on the ketone.
Stem Cells to the Rescue: Development and Application of Cell-Based Therapy for Microvascular Repair
Authors: Lerman Lilach O. , Lerman Amir
Published: August 2021

The microcirculation includes an invisible network of micro-vessels that are up to a few hundred microns in diameter [...]
Identifying the Effects of Reactive Oxygen Species on Mitochondrial Dynamics and Cytoskeleton Stability in Dictyostelium discoideum
Authors: Downs Evan , Bottrell Amber D. , Naylor Kari
Published: August 2021

Defects in mitochondrial dynamics, fission, fusion, and motility have been implicated in the pathogenesis of multiple neurodegenerative diseases, including Parkinson’s disease, Alzheimer’s disease, Huntington’s disease, and Charcot–Marie–Tooth disease. Another key feature of neurodegeneration is the increase in reactive oxygen species (ROS). Previous work has shown that the cytoskeleton, in particular the microtubules, and ROS generated by rotenone significantly regulate mitochondrial dynamics in Dictyostelium discoideum. The goal of this project is to study the effects of ROS on mitochondrial dynamics within our model organism D. discoideum to further understand the underlying issues that are the root of neurodegenerative diseases such as Alzheimer’s disease and Parkinson’s disease. We chose three likely ROS inducers, cumene hydroperoxide, hydroxylamine hydrochloride, and Antimycin A. Our work demonstrates that alteration of the microtubule cytoskeleton is not required to alter dynamics in response to ROS and there is no easy way to predict how mitochondrial dynamics will be altered based on which ROS generator is used. This research contributes to the better understanding of the cellular mechanisms that induce the pathogenesis of incurable neurodegenerative diseases with the hope that it will translate into developing new and more effective treatments for patients afflicted by them.
Evaluation of Photosensitive Paper Coatings as Detectors for Instrumentation-Free UV Photometric Analysis Based on Photography-Based Photometry
Authors: Choleva Tatiana G. , Sfakianaki Afroditi , Vlessidis Athanasios G. , Giokas Dimosthenis L.
Published: August 2021

Photography-based photometry is a technique developed to perform high throughput UV photometric analysis without instrumental detectors in resource-limited settings. Its principle relies on the illumination of a sample with UV irradiation and then capturing the transmitted irradiation on a photosensitive paper surface. Therefore, the photosensitive surface acts as a detector for the determination of the concentration of analytes in the sample. This work aims to investigate the optimum photosensitive paper coatings for capturing the transmitted UV irradiation. To this end, photosensitive coatings based on silver, iron, and dichromate salts were tested using three assays of pharmaceutical and biochemical interest. The results from both calibrations, using standard solutions and the application in real samples, show that photosensitive coatings based on iron salts provide the best results. Importantly, the detection limits and the linear range of the calibration curves were better than those obtained with standard photometry. Based on these findings, cyanotype green papers, are proposed as optimum detectors for photography-based photometry. This finding simplifies the operation of the technique enabling the fabrication of prototype readers for analytical assays performed in resource limited settings, point-of-need applications or in the field.
Improvement of the Tribological Properties of Alumina Coatings by Zirconia Addition
Authors: Mathew Jacob Shiby , Marcinauskas Liutauras , Kalin Mitjan , Kėželis Romualdas , Kavaliauskas Žydrūnas , Gecevičius Giedrius , Čapas Vytautas
Published: August 2021

Al2O3 and Al2O3-ZrO2 coatings were deposited on stainless steel using atmospheric plasma spraying. The influence of arc current and zirconia addition on the surface morphology of the coating, phase composition and tribological properties under dry sliding conditions were investigated. The addition of zirconia reduced the surface roughness of the coatings. The X-ray diffraction measurements indicated that the Al2O3 coatings were composed of β-Al2O3, α-Al2O3, and γ-Al2O3 phases. The addition of zirconia led to the formation of tetragonal and monoclinic phases of zirconia in the as-sprayed coatings. The friction coefficients of Al2O3 and Al2O3-ZrO2 coatings were similar and varied in the range of 0.72–0.75. The specific wear rates of the as-sprayed coatings were reduced with the increase of arc current. It was obtained that the wear rates of the Al2O3-ZrO2 coatings were at least three times lower compared to Al2O3 coatings.
Assessment of Gradient Descent Trained Rule-Fact Network Expert System Multi-Path Training Technique Performance
Authors: Straub Jeremy
Published: August 2021

The use of gradient descent training to optimize the performance of a rule-fact network expert system via updating the network’s rule weightings was previously demonstrated. Along with this, four training techniques were proposed: two used a single path for optimization and two use multiple paths. The performance of the single path techniques was previously evaluated under a variety of experimental conditions. The multiple path techniques, when compared, outperformed the single path ones; however, these techniques were not evaluated with different network types, training velocities or training levels. This paper considers the multi-path techniques under a similar variety of experimental conditions to the prior assessment of the single-path techniques and demonstrates their effectiveness under multiple operating conditions.
Synthesis, Single Crystal Structural Investigation, Hirshfeld Surface Analysis, Thermoanalysis and Spectroscopic Study of Two New Cu(II) and Co(II) Transition-Metal Complexes
Authors: Boubakri Rim , Szybowicz Mirosław , Sadej Mariola , Soudani Sarra , Lefebvre Frédéric , Ferretti Valeria , Ben Nasr Cherif , Kaabi Kamel
Published: August 2021

Two new complexes, [Cu(dimpyr)2(H2O)2](NO3)2.2H2O (1) and (Hamdimpy)2[CoCl4].H2O (2), with the monodentate ligand 2-amino-6-methylpyrimidin-4-(1H)-one (dimpyr) and the countercation 4-amino-2,6-dimetylpyrimidium (Hamdimpy), respectively, were prepared and characterized by single crystal X-ray diffraction, elemental analysis and IR spectroscopy. In (1), the Cu(II) cation is tetracoordinated, in a square plan fashion, by two nitrogen atoms from the pyrimidine ring of the organic ligand and two oxygen atoms of two coordinated water molecules. In the atomic arrangement, the CuO2N2 square planes are interconnected via the formation of O-H…O hydrogen bonds involving both coordinated and free water molecules and NO3− nitrate anions to form inorganic layers parallel to the (a, b) plane at z = (2n + 1)/4. In (2), the central atom Co(II) is four-coordinated in a distorted tetrahedral fashion by four Cl− ions. The [CoCl4]2− tetrahedra are arranged parallel to the plane (1¯10) at x = (2n + 1)/2 and the organic cations are grafted between them by establishing with them hydrogen bonds of CH…Cl and NH…Cl types. The vibrational absorption bands were identified by infrared and Raman spectroscopy. Intermolecular interactions were investigated via Hirshfeld surfaces and electronic properties such as HOMO and LUMO energies were derived. The two compounds were characterized by thermal analysis to determine their thermal behavior with respect to temperature.
Heat-Induced Transformation of Luminescent, Size Tuneable, Anisotropic Eu:Lu(OH)2Cl Microparticles to Micro-Structurally Controlled Eu:Lu2O3 Microplatelets
Authors: Fellner Madeleine , Soppelsa Alberto , Lauria Alessandro
Published: August 2021

Synthetic procedures to obtain size and shape-controlled microparticles hold great promise to achieve structural control on the microscale of macroscopic ceramic- or composite-materials. Lutetium oxide is a material relevant for scintillation due to its high density and the possibility to dope with rare earth emitter ions. However, rare earth sesquioxides are challenging to synthesise using bottom-up methods. Therefore, calcination represents an interesting approach to transform lutetium-based particles to corresponding sesquioxides. Here, the controlled solvothermal synthesis of size-tuneable europium doped Lu(OH)2Cl microplatelets and their heat-induced transformation to Eu:Lu2O3 above 800 °C are described. The particles obtained in microwave solvothermal conditions, and their thermal evolution were studied using powder X-ray diffraction, scanning electron microscopy (SEM), transmission electron microscopy (TEM), optical microscopy, thermogravimetric analysis (TGA), luminescence spectroscopy (PL/PLE) and infrared spectroscopy (ATR-IR). The successful transformation of Eu:Lu(OH)2Cl particles into polycrystalline Eu:Lu2O3 microparticles is reported, together with the detailed analysis of their initial and final morphology.
Understanding Diversity and Systematics in Australian Fabaceae Tribe Mirbelieae
Authors: Barrett Russell L. , Clugston James A. R. , Cook Lyn G. , Crisp Michael D. , Jobson Peter C. , Lepschi Brendan J. , Renner Matthew A. M. , Weston Peter H.
Published: August 2021

Australia has a very diverse pea-flowered legume flora with 1715 native and naturalised species currently recognised. Tribe Mirbelieae s.l. includes 44% of Australia’s peas in 24 genera with 756 recognised species. However, several genera within the Pultenaea alliance in tribe Mirbelieae are considered to be non-monophyletic and two main options have been proposed: option one is to merge ca. 18 genera containing ca. 540 species (the largest genus, Pultenaea has nomenclatural priority); and option two is to re-circumscribe some genera and describe new genera as required to form monophyletic groups. At the species level, option one would require 76% of names to be changed; whereas based on available data, option two is likely to require, at most, 8.3% of names to change. Option two therefore provides the least nomenclatural disruption but cannot be implemented without a robust phylogenetic framework to define new generic limits. Here we present novel analyses of available plastid DNA data (trnL-F) which suggest that option two would be feasible once sufficient data are generated to resolve relationships. However, the reticulate evolutionary histories or past rapid speciation suggested for this group may prevent the resolution of all nodes. We propose targeted use of Next-Generation Sequencing technology as the best way to resolve relationships between the key clades in the tribe and present a framework for such a study. An overview of current taxonomy in the tribe is presented, along with the state of taxonomic knowledge and availability of published descriptions for electronic flora treatments. Several new combinations and typifications are published in an appendix.
Mitofusin-2 Down-Regulation Predicts Progression of Non-Muscle Invasive Bladder Cancer
Authors: Cormio Antonella , Busetto Gian Maria , Musicco Clara , Sanguedolce Francesca , Calò Beppe , Chirico Marco , Falagario Ugo Giovanni , Carrieri Giuseppe , Piccoli Claudia , Cormio Luigi
Published: August 2021

Identification of markers predicting disease outcome is a major clinical issue for non-muscle invasive bladder cancer (NMIBC). The present study aimed to determine the role of the mitochondrial proteins Mitofusin-2 (Mfn2) and caseinolytic protease P (ClpP) in predicting the outcome of NMIBC. The study population consisted of patients scheduled for transurethral resection of bladder tumor upon the clinical diagnosis of bladder cancer (BC). Samples of the main bladder tumor and healthy-looking bladder wall from patients classified as NMIBC were tested for Mfn2 and ClpP. The expression levels of these proteins were correlated to disease recurrence, progression. Mfn2 and ClpP expression levels were significantly higher in lesional than in non-lesional tissue. Low-risk NMIBC had significantly higher Mfn2 expression levels and significantly lower ClpP expression levels than high-risk NMIBC; there were no differences in non-lesional levels of the two proteins. Lesional Mfn2 expression levels were significantly lower in patients who progressed whereas ClpP levels had no impact on any survival outcome. Multivariable analysis adjusting for the EORTC scores showed that Mfn2 downregulation was significantly associated with disease progression. In conclusion, Mfn2 and ClpP proteins were found to be overexpressed in BC as compared to non-lesional bladder tissue and Mfn2 expression predicted disease progression.
Structural and Parametric Optimization of S–CO2 Nuclear Power Plants
Authors: Rogalev Nikolay , Rogalev Andrey , Kindra Vladimir , Komarov Ivan , Zlyvko Olga
Published: August 2021

The transition to the use of supercritical carbon dioxide as a working fluid for power generation units will significantly reduce the equipment′s overall dimensions while increasing fuel efficiency and environmental safety. Structural and parametric optimization of S–CO2 nuclear power plants was carried out to ensure the maximum efficiency of electricity production. Based on the results of mathematical modeling, it was found that the transition to a carbon dioxide working fluid for the nuclear power plant with the BREST–OD–300 reactor leads to an increase of efficiency from 39.8 to 43.1%. Nuclear power plant transition from the Rankine water cycle to the carbon dioxide Brayton cycle with recompression is reasonable at a working fluid temperature above 455 °C due to the carbon dioxide cycle′s more effective regeneration system.
Improving Seismic Inversion Robustness via Deformed Jackson Gaussian
Authors: Silva Suzane A. , da Silva Sérgio Luiz E. F. , de Souza Renato F. , Marinho Andre A. , de Araújo João M. , Bezerra Claudionor G.
Published: August 2021

The seismic data inversion from observations contaminated by spurious measures (outliers) remains a significant challenge for the industrial and scientific communities. This difficulty is due to slow processing work to mitigate the influence of the outliers. In this work, we introduce a robust formulation to mitigate the influence of spurious measurements in the seismic inversion process. In this regard, we put forth an outlier-resistant seismic inversion methodology for model estimation based on the deformed Jackson Gaussian distribution. To demonstrate the effectiveness of our proposal, we investigated a classic geophysical data-inverse problem in three different scenarios: (i) in the first one, we analyzed the sensitivity of the seismic inversion to incorrect seismic sources; (ii) in the second one, we considered a dataset polluted by Gaussian errors with different noise intensities; and (iii) in the last one we considered a dataset contaminated by many outliers. The results reveal that the deformed Jackson Gaussian outperforms the classical approach, which is based on the standard Gaussian distribution.
Organizational Differences among Universities in Three Socioeconomic Contexts: Finland, Spain and Ecuador. Relational Coordination Approach
Authors: Checa-Morales Cristina , De-Pablos-Heredero Carmen , Carreño Angela Lorena , Haider Sajid , García Antón
Published: August 2021

The knowledge of local culture is essential to establish competitive strategies in higher education. The objective of this research was to identify the organizational differences among three universities with different international contexts and satisfaction level. An approach was made regarding Relational Coordination (RC) attributes: accurate, frequent and problem-solving communication, shared knowledge, mutual respect and shared goals, by discriminant analysis method. A random sample of 300 students, 100 belonging to each university, was surveyed on the 23 RC variables in 2017–2018. First, the RC variables were evaluated by general linear model (GLM). The three universities—Arcada University of Applied Science (ARCADA) in Finland, University of Cordoba (UCO) in Spain and Agricultural Polytechnic of Manabi “MFL” (ESPAM) in Ecuador—and the two levels of student satisfaction—Low and High—were used as fixed factors. Second, a discriminant model was built with RC variables. A higher level of RC practices concerning to accurate, frequent and problem-solving communication achieved higher levels of satisfaction, regardless of the universities’ socioeconomic context. RC differentiation among three universities showed that shared goals with lecturers and administrative officers and problem-solving communication among classmates were the variables with the highest discriminant power. Two clusters were obtained, where UCO was the most differentiated university. In conclusion, organizational practices made a difference among the three universities. Discriminant analysis can be adapted and extended to different universities to improve quality.
Estimating the Memory Consumption of a Hardware IP Defragmentation Block
Authors: Czekaj Maciej , Jamro Ernest , Wiatr Kazimierz
Published: August 2021

IP fragmentation is still prevalent on the Internet. Defragmented traffic is a prerequisite for many network processing algorithms. This work focuses on the size and organization of a flow table, which is an essential ingredient of the hardware IP defragmentation block. Previous research suggests that fragmented IP traffic is highly local, and a relatively small flow table (on the order of a thousand entries) can process most of the traffic. Samples of IP traffic were obtained from public data sources and used for a statistical analysis, revealing the key factors in achieving design goals. The findings were backed by an extensive design space exploration of the software defragmentation model, which resulted in the efficiency estimates. To provide a robust score of the simulation model, a new validation technique is employed that helps to overcome the limitations of the samples.
Detection and Classification of Defective Hard Candies Based on Image Processing and Convolutional Neural Networks
Authors: Wang Jinya , Li Zhenye , Chen Qihang , Ding Kun , Zhu Tingting , Ni Chao
Published: August 2021

Defective hard candies are usually produced due to inadequate feeding or insufficient cooling during the candy production process. The human-based inspection strategy needs to be brought up to date with the rapid developments in the confectionery industry. In this paper, a detection and classification method for defective hard candies based on convolutional neural networks (CNNs) is proposed. First, the threshold_li method is used to distinguish between hard candy and background. Second, a segmentation algorithm based on concave point detection and ellipse fitting is used to split the adhesive hard candies. Finally, a classification model based on CNNs is constructed for defective hard candies. According to the types of defective hard candies, 2552 hard candies samples were collected; 70% were used for model training, 15% were used for validation, and 15% were used for testing. Defective hard candy classification models based on CNNs (Alexnet, Googlenet, VGG16, Resnet-18, Resnet34, Resnet50, MobileNetV2, and MnasNet0_5) were constructed and tested. The results show that the classification performances of these deep learning models are similar except MnasNet0_5 with the classification accuracy of 84.28%, and the Resnet50-based classification model is the best (98.71%). This research has certain theoretical reference significance for the intelligent classification of granular products.