bluuebunny/crossref_metadata_2025 huggingface dataset:
Investigation of Relationship between Playing the Piano Capability and the Second-to-Fourth Digit (2D:4D) Ratio
Authors: Ayşe Gül Kabakci  , Memduha Gülhal Bozkir
Published: August 2023

BACKGROUND

In the field of piano playing, the suitability of physical characteristics of individuals, especially at the beginner level, is one of the first criteria considered important for educators. In many studies examining the physical fitness of individuals in sports fields, the importance of the relationship between physical fitness and ability has been revealed. In addition, there are studies examining the physical fitness for playing any instrument in the field of music. However, studies examining the physical characteristics of beginner piano players are very limited. Therefore, the purpose of our study was to investigate the relationship between the physical properties of the hand and the ability to play the piano. In addition, our study is the first to examine the relationship between the second digit and fourth digit ratio and the state of being successful in playing the piano. We also intended to contribute to the literature on this subject.

METHODS

Among the students who participated in the ballet and piano special talent exam held at Cukurova University State Conservatory in the 2013 and 2015 academic years, students who participated in the piano special talent exam were included in the study. In this exam, the suitability of the candidate to play the piano was evaluated in terms of musical and physical competence. In our study, the second digit and fourth digit length parameters, which include the physical characteristics of the hand, were evaluated. Length measurements were made with a digital display caliper. Students between the ages of 8 and 11 who participated in the piano talent exam were divided into two groups as those who were successful and those who were unsuccessful in the talent test.

RESULTS

The number of successful girls was 20 and the average length of the second digit 6.00±0.50 cm, mean fourth digit length; 5.90±0.40 cm, the number of unsuccessful girls was 17, the average of the second length; 5.98±0.48 cm, and the mean fourth digit length was 5.89±0.48 cm. The number of successful men was 15, and the mean length of the second digit; 6.55±0.36 cm, mean fourth digit length; 6.61±0.47 cm, the number of unsuccessful men was 17, the mean length of the second digit; 6.46±0.52 cm, mean fourth digit length; 6.52±0.55 cm were found.

CONCLUSIONS

There was no significant relationship between the piano playing ability and the second digit and fourth digit length ratios. We think that this is due to the evaluation of factors such as the level of development of the sensorimotor and neuromuscular system, the level of auditory proficiency, and coordination, not only with the anatomical suitability factors, in the scoring of the talent exam. Therefore, we can say that being successful in playing the piano does not depend only on digit lengths.
Iliac Artery–Inferior Vena Cava–Duodenal Fistula: An Unusual Cause of Massive Gastrointestinal Bleeding
Authors: Karna Rahul , Jagdish Balaji , Mohy-Ud-Din Nabeeha , Kulkarni Abhijit
Published: August 2023

An arteriovenous-enteric fistula is a 3-way connection between the vascular and enteric system and associated with high mortality. We describe a case of iliac artery–inferior vena cava–duodenal fistula in a young female with a retroperitoneal mass presenting with sepsis and hemorrhagic shock with a catastrophic clinical course. These fistulas can be missed on endoscopy/colonoscopy and are usually diagnosed on computed tomography angiogram of the abdomen. Complex vasculoenteric fistula should be among differentials in patients presenting with gastrointestinal bleeding, especially with a history of malignancy, radiation, foreign bodies, and trauma. The management is complex and should involve a multidisciplinary approach involving vascular surgery, intervention radiology, and gastroenterologist.
Electrical and Optical Investigation of an Electric Arc in Hydrogen for short gaps
Authors: Najam Ammar , Methling Ralf , Hummel Jan , Gonzalez Diego , Uhrlandt Dirk
Published: August 2023

Hydrogen or mixtures containing hydrogen represent attractive gases for low-voltage switching devices because of increased arc quenching behaviour. However, fundamental electrical properties of arcs in hydrogen are still not well known. In this paper, first results of a study of a DC switching arc in pure hydrogen at 1 bar between graphite electrodes are presented for low currents of 8 and 16 A. The arc voltage and current are measured during contact separation. High-speed images of the arc are processed to determine the arc length considering the high arc dynamics with erratic elongations and jumps of the electrode attachment. The arc voltage dependence on the length results in a typical sheath voltage of approximately 23 V and mean electric fields in the arc column of 18.7 V/mm at 8 A and 10.8 V/mm at 16A.
O impacto dos instrumentos endodônticos ProDesign M no ensino de graduação
Authors: Silva Polliana Lara Araújo , Neves Gabriel da Silva Maciel , Praxedes Lorena Ferreira Soares , Castro Fabrício Luscino Alves de , Bruno Kely Firmino
Published: August 2023

Introdução: O planejamento educacional da disciplina de Endodontia na graduação deve incorporar novas técnicas, para a melhoria do processo formativo. Assim, os instrumentos manuais de níquel-titânio ProDesign M (PDM) trouxeram uma proposta inovadora de preparo do canal radicular. Objetivo: O presente estudo teve por objetivo avaliar o impacto da introdução da PDM no ensino da graduação, por meio da percepção e satisfação de docentes e discentes. Métodos: 54 docentes e 285 discentes de faculdades de Odontologia do Brasil responderam a um questionário online de múltipla escolha, com perguntas comparativas entre a técnica de preparo manual usada antes da PDM (técnica associando brocas de preparo cervical e instrumentos de aço inox e/ou técnica associando brocas de preparo cervical e instrumentos de NiTi convencionais) e a técnica com PDM. Resultados: Os docentes tiveram maior facilidade de ensinar a técnica com PDM (92,6%), com superior aprendizado dos alunos (94,4%), obtenção de melhor qualidade de tratamento endodôntico (90,7%), com facilitação da técnica (90,7%), menor tempo de trabalho (96,3%), menor fadiga (96,3%) e com maior interesse dos alunos (94,4%). Os discentes relataram que a técnica com PDM permitiu maior facilidade (89,8%) e segurança (93%) para execução, melhor qualidade do tratamento endodôntico (90,9%), com menor tempo de trabalho (96,5%) e menor fadiga (95,8%), sendo a técnica de escolha dos discentes para tratamentos endodônticos futuros (96,5%). Conclusões: A introdução dos instrumentos PDM no ensino da graduação foi positiva, com a satisfação de docentes e discentes, e otimização do processo de ensino-aprendizagem.
Regeneração óssea guiada em apicectomia: relato de caso clínico
Authors: Menuci Neto Angelo , Reston Eduardo , Barletta Fernando Branco , Pelegrini Graziella
Published: August 2023

Introdução: A apicectomia é o procedimento de escolha quando o tratamento endodôntico convencional não é possível, devido à dificuldade em se obter acesso ou por lesões refratárias. A abordagem cirúrgica permite o debridamento do tecido patológico, resultando em uma cavidade livre de exsudato. Na presença de lesões periapicais extensas, o tratamento endodôntico cirúrgico combinado à regeneração óssea guiada é uma técnica que permite o acesso direto à lesão e promove a neoformação óssea por meio de enxertos com biomaterial. Objetivo: O objetivo do presente relato de caso clínico foi apresentar os resultados da cirurgia periapical com enxerto ósseo bovino inorgânico (Bio-Oss®). Relato do caso: Paciente com 59 anos de idade, com uma extensa lesão periapical no dente #11. Resultados: No acompanhamento clínico e radiográfico de dois anos, houve formação e reparo de tecido ósseo. Conclusões: Conclui-se que o uso do biomaterial Bio-Oss® pode ser um fator determinante para a regeneração do osso alveolar e lesões no ligamento periodontal, além de permitir o reparo do cemento e impedir a deposição de tecido fibroso na cavidade defeituosa.
Avaliação do diâmetro e conicidade de diferentes cones de guta-percha
Authors: Pinto Jader Camilo , Oliveira Caroline Castilho de , Torres Fernanda Ferrari Esteves , Tanomaru Juliane Maria Guerreiro , Tanomaru Filho Mario
Published: August 2023

Objetivo: O objetivo do presente estudo foi comparar o diâmetro da ponta e a conicidade de diferentes marcas de cones de guta-percha, em comparação ao tamanho nominal 25.06. Métodos: Foram utilizados cones de guta-percha Tanari 25.06, Diadent .06, Odous de Deus Fine-Medium e Medium (n = 50). Os cones Diadent e Odous de Deus foram calibrados com régua endodôntica no diâmetro de ponta de 0,25 mm. Em seguida, todos os cones de guta-percha foram medidos usando um perfilômetro (Profile Projector Nikon, modelo 6C-2) na ponta (D0), a 3 mm da ponta (D3) e a 6 mm da ponta (D6). Os dados obtidos para o diâmetro e a conicidade foram comparados com valores nominais de 0,25 mm de diâmetro da ponta e 0,06 de conicidade. Os testes ANOVA e Tukey foram realizados com significância de 5%. Resultados: Não houve diferença quanto ao diâmetro da ponta e à conicidade entre o Tanari 25.06 e o tamanho nominal (p > 0,05). Todos os cones acessórios de guta-percha apresentaram diâmetro da ponta semelhante ao tamanho nominal (p > 0,05). Entretanto, Odous de Deus Fine-Medium apresentou conicidade abaixo do tamanho nominal no D3 (0,045) e no D6, e o Diadent .06 apresentou conicidade abaixo do tamanho nominal no D3. O Odous de Deus Medium apresentou conicidade acima do tamanho nominal no D6 (p < 0,05). Conclusões: Os cones Tanari 25.06 apresentam padronização ideal de ponta e conicidade. O uso de uma régua calibradora endodôntica promoveu um diâmetro adequado da ponta.
Abordagem conservadora de um incisivo central com trinca na face vestibular: relato de caso com acompanhamento de dois anos
Authors: Iglesias Julia Eick , Souza Fabio Herrmann Coelho de , Grecca Fabiana Soares
Published: August 2023

Introdução: Dentes com trincas têm uma abordagem difícil e multidisciplinar. O tratamento minimamente invasivo é o novo padrão na Odontologia para manter a estrutura dentária e permitir a cicatrização tecidual. Objetivo: Relatar a abordagem conservadora e o acompanhamento de dois anos de um dente traumatizado não vital com trinca na face vestibular e com envolvimento periodontal, submetido a tratamento cirúrgico, endodôntico e restaurador. Relato de caso: Paciente do sexo feminino, com 21 anos de idade, chegou ao Hospital Odontológico da Faculdade de Odontologia da Universidade Federal do Rio Grande do Sul, com histórico de traumatismo dentário há dez anos na região anterossuperior. O exame clínico revelou fratura da coroa e trinca na face vestibular do dente #11. A radiografia periapical mostrou que os dentes #11 e #21 apresentavam lesão periapical, e ambos tiveram resposta negativa ao teste de sensibilidade pulpar. Um retalho periodontal foi realizado para acessar e preencher a trinca com resina de baixa viscosidade. No tratamento endodôntico de ambos os dentes, utilizou-se o hidróxido de cálcio como medicação intracanal. Após a obturação do canal radicular, uma matriz de silicone foi utilizada para realizar a restauração dos dentes. Um pino anatômico de fibra de vidro foi cimentado no dente #11, juntamente com uma reconstrução em resina. No dente #21, foi realizada restauração de resina composta. Resultados: O acompanhamento clínico e radiográfico de dois anos revelou ausência de sintomas e mostrou cicatrização da região periapical. Conclusão: Até o presente momento, o sucesso do caso devolveu saúde, função e estética do dente e da paciente, de forma conservadora.
Resistência à fadiga cíclica de instrumentos reciprocantes de níquel-titânio com diferentes tratamentos térmicos e secções transversais
Authors: Pinto Jader Camilo , Leme Giovanni Garcia , Santos Junior Airton Oliveira , Tanomaru Juliane Maria Guerreiro , Tanomaru Filho Mario
Published: August 2023

Objetivo: O objetivo do presente estudo foi comparar a resistência à fadiga cíclica de instrumentos reciprocantes de níquel-titânio (NiTi) tratados termicamente. Métodos: Instrumentos reciprocantes de NiTi com diferentes tratamentos térmicos — ProDesign R 25/.06 (PDR), Reciproc Blue 25/.08 (RB), X1 File 25/ 06 (X1), WaveOne Gold (WOG) 25/.07 e Unicone 25/.06 (UN) — foram avaliados (n = 12). O teste de resistência à fadiga cíclica foi realizado em um canal artificial de aço inoxidável, com ângulo de curvatura de 60° e raio de 5 mm localizado a 5 mm da ponta (n = 12). Os instrumentos foram acionados por um motor elétrico no modo reciprocante até a ocorrência de fratura. O tempo até a fratura foi registrado, e os fragmentos foram medidos. Testes ANOVA e Tukey foram realizados, com nível de significância de 5%. Resultados: PDR apresentou maior fadiga cíclica do que os demais instrumentos (p < 0.05), seguido pelos instrumentos RB e X1. WOG e UN apresentaram os menores valores de fadiga cíclica (p < 0.05). Conclusão: Diferentes secções transversais e tratamentos térmicos influenciam a resistência à flexão de instrumentos reciprocantes de NiTi.
Estudo comparativo in vitro da extrusão apical de detritos e da resistência à fratura radicular de dentes preparados com três diferentes sistemas de limas
Authors: Abdo Salma B. A.
Published: August 2023

Objetivo: O presente estudo teve como objetivo medir a quantidade de extrusão apical de detritos e a resistência à fratura radicular de canais preparados usando três diferentes sistemas de limas. Métodos: Foram usados 120 pré-molares inferiores unirradiculares divididos aleatoriamente em quatro grupos (n = 30 dentes/grupo). O Grupo 4 serviu como controle positivo para a segunda parte do estudo. Cada grupo do 1 ao 3 foi instrumentado usando um dos seguintes sistemas de limas: Twisted File Adaptive (TFA), TruNatomy (TRN) e limas manuais tipo K. Os detritos extruídos de cada grupo foram coletados em tubos Eppendorf previamente pesados, armazenados em uma incubadora a 70°C por três dias, e depois pesados. A análise estatística foi feita usando a análise de variância e o teste post-hoc de Tukey. Além disso, os grupos 1 a 3 foram obturados com guta-percha e AH Plus Sealer. Em seguida, a resistência à fratura dos quatro grupos foi medida usando uma máquina de teste Instron. O software SPSS (v. 25, for Windows, SPSS, Chicago, EUA) foi utilizado para a análise estatística. Resultados: O sistema de limas TRN produziu significativamente menos detritos do que o sistema de limas TFA e as limas manuais tipo K (p < 0,001). Além disso, diferenças sem significância estatística na resistência à fratura foram observadas entre os três grupos experimentais. Conclusão: O sistema TRN apresentou extrusão de detritos significativamente menor do que o sistema TFA e as limas manuais tipo K, e teve maior resistência à fratura, mas sem diferenças significativas para o sistema TFA e as limas manuais tipo K.
Quando a dor não é de origem dentária: qual a importância para o endodontista?
Authors: Lima Netto Beatriz Amaral de , Salbego Rafaela Stocker , Ferreira Matheus Herreira , Bonjardim Leonardo Rigoldi
Published: August 2023

No dia a dia do consultório, as queixas de dor correspondem às principais razões pelas quais os pacientes buscam atendimento. Quadros dolorosos de origem dentária que podem impactar a qualidade de vida — como pulpites reversíveis ou irreversíveis, pericoronarites, fraturas dentárias, cáries profundas, abscessos odontogênicos e hipersensibilidade dentinária — são exemplos mais comuns e, em sua maioria, não costumam apresentar uma grande dificuldade em seu diagnóstico, sendo facilmente identificados por meio de exame clínico e/ou auxílio de exames complementares.
Análise da adaptação marginal e porosidade do MTA e MTA Flow na presença de sangue
Authors: Santos Mariana Parizato , Westphal Luana , Almeida Josiane de , Schuldt Daniela Peressoni Vieira , Jussiani Eduardo Inocente , Andrello Avacir Casanova , Coelho Beatriz Serrato
Published: August 2023

Objetivo: O objetivo do presente estudo foi analisar, por meio de microtomografia computadorizada, a adaptação marginal e a porosidade dos cimentos MTA (Agregado de Trióxido Mineral) e MTA Flow na presença de sangue. Material e Métodos: As raízes de 48 incisivos bovinos medindo 10 mm foram limpas e preenchidas com dois cimentos: MTA e MTA Flow. Após o preenchimento da porção apical, as amostras foram separadas em dois subgrupos, de acordo com o meio de armazenamento (sangue/soro), e retidas em ambiente controlado, por sete dias. Todas as amostras foram submetidas à análise de microtomografia computadorizada, para avaliar os parâmetros de adaptação marginal, número de poros e porcentagem de porosidade. O teste ANOVA de duas vias foi aplicado para o tipo de cimento e meio de armazenamento, e o teste post-hoc de Tukey, com nível de significância de 5%, para as comparações pareadas. Resultados: O percentual de poros fechados e a adaptação marginal não mostraram diferença entre os grupos. O número de poros no MTA foi maior na presença de sangue (p = 0,002). O grupo MTA apresentou porosidade na presença de sangue (p = 0,008), mas essa foi menor do que no grupo MTA Flow (p = 0,006). Conclusão: Na presença de sangue, o MTA Flow mostrou menos poros do que o MTA. O sangue também melhorou a porcentagem de porosidade total do MTA, que apresentou melhores resultados do que o MTA Flow nesse parâmetro.
Avaliação comparativa in vitro da solubilidade e resistência da união ao microcisalhamento usando nanoformulações de ProRoot MTA e cimento Portland
Authors: Nashaat Yousra Mohamed , Roshdy Nehal Nabil
Published: August 2023

Objetivos: Este estudo investigou e comparou a solubilidade e a resistência da união ao microcisalhamento do cimento ProRoot MTA (PMTA) e do cimento Portland (CP) disponíveis comercialmente com nanoformulações de cimento ProRoot MTA (NPMTA) e de cimento Portland (NCP), na tentativa de desenvolver um novo material para obturação de canais radiculares. Métodos: O teste de solubilidade foi realizado em 48 espécimes com anéis de PVC preenchidos com os cimentos testados: PMTA, CP, NPMTA e NCP. Os espécimes foram embebidos em água destilada e pesados a cada tempo experimental (1, 3 e 7 dias). A solubilidade foi testada calculando-se a diferença de massa entre a primeira e a segunda leituras. O teste de resistência da união ao microcisalhamento foi realizado em 48 espécimes de dentina criados a partir de pré-molares extraídos, divididos em quatros grupos de acordo com os cimentos testados. Uma carga de cisalhamento com modo de força de tração foi aplicada por uma máquina de ensaios e a resistência da união foi medida. O teste ANOVA foi aplicado para investigar dados com distribuição normal, seguido pelo teste post-hoc de Tukey para comparação pareada. Resultados: Os quatro grupos não apresentaram diferença significativa entre os resultados do teste de solubilidade no dia 1 (p =0,593), no dia 7 (p =0,976) e no dia 14 (p =0,567). Houve uma diferença estatisticamente significativa nos valores médios da resistência da união ao microcisalhamento dos quatro grupos (p < 0,001). Houve um aumento significativo na resistência da união entre o PMTA e o NPMTA (p =0,004), e o CP e o NCP (p <0,001). Conclusão: As nanoformulações de PMTA e CP mostraram uma melhora nas propriedades mecânicas dos cimentos. O NPMTA e o NCP mostraram solubilidade comparável e melhores resultados para resistência da união ao microcisalhamento do que o PMTA.
Avaliação da limpeza e eficácia antimicrobiana da ablação a laser com verde de indocianina contra biofilme de Enterococcus faecalis em canais radiculares
Authors: Leonardo Renato de Toledo , Cintra Luciano Tavares Angelo , Jacinto Rogério de Castilho , Loureiro Caroline , Banci Henrique Augusto , Ribeiro Ana Paula Fernandes , Faria Flávio Duarte , Jensen Steve , Larsen Rob , Cao Densen
Published: August 2023

Objetivos: Avaliar a limpeza e a eficácia antimicrobiana do laser infravermelho com verde de indocianina (ICG) na eliminação do biofilme de E. faecalis no conduto radicular. Métodos: E. faecalis foi inoculado e incubado por 14 dias em 20 dentes humanos unirradiculados. Então, foi realizada coleta inicial (S1) e as raízes foram divididas aleatoriamente em dois grupos (n=10): G1) ICG + laser (810nm); G2) hipoclorito de sódio 2,5% (NaOCl). Os canais foram preparados com lima única X1 Blue e irrigados com solução salina no G1 e NaOCl no G2. No G1, os canais receberam ICG + laser e a segunda coleta (S2) foi realizada. O número de unidades formadoras de colônias (UFC) foi obtido e foi aplicado o teste t de Student (p<0,05). As raízes foram clivadas longitudinalmente e analisadas em microscópio eletrônico de varredura (MEV) para avaliar a limpeza. O teste U de Mann- -Whitney foi aplicado (p<0,05). Resultados: Os dois grupos apresentaram ausência de crescimento bacteriano após protocolos de tratamento endodôntico, e não foram observadas diferenças significativas entre os grupos quanto à redução do biofilme de E. faecalis (p>0,05). Na análise em MEV, todos os grupos alcançaram a pontuação média 1 ou 2 nos terços coronário e médio. Para o terço apical, o grupo G1 apresentou escores 1 ou 2, e o grupo G2 apresentou escores de 1 a 3, sem diferença estatística (p>0,05). Conclusão: O procedimento de ablação a laser com verde de indocianina e o uso do hipoclorito foram semelhantes quanto à limpeza e eliminação do biofilme de E. faecalis no canal radicular.
KENTLERDE MEKÂNSAL AYRIŞMANIN SOSYAL VE SİYASAL TEMELLERİ: ERZİNCAN ÖRNEĞİ
Authors: BAKİOĞLU Akın , TİTİZ Başak
Published: August 2023

Bu çalışma kent sosyolojisi perspektifinden mekânsal ayrışmanın sosyal ve siyasal temellerini Erzincan kenti örneğinde ele almaktadır. Nitel araştırma yöntemlerinden yararlanan araştırma, Erzincan’da ortaya çıkan mekânsal farklılaşmayı tarihsel ve güncel politikalar ekseninde tartışmaktadır. Kentlerin mekânsal ayrışma sürecinde etnisite temelli olarak dışlanan gruplar kent yoksulluğunun temel bileşenini oluşturmaktadır. Araştırma; neoliberal kent politikalarının bir sonucu olan kentsel dönüşümlerin derinleştirdiği eşitsizliklerin kentsel etnisite ve sosyal dışlama ile olan bağını ve ulusaşırı göç politikalarının kentsel yaşam alanını nasıl şekillendirdiğini ortaya koymaktadır. Günümüzde kentsel etnisite, kentsel dönüşüm politikaları, yerinden etme, soylulaştırma ve göç politikaları ile şekillenmektedir. Bu politikaların sonucu oluşan kentsel yapıdaki eşitsizlik derinleşmektedir ve etno-kültürel ayrımlara eşlik eden sınıfsal farklılıklar belirginleşmektedir. Bu çerçeveden hareket eden araştırma Erzincan kenti örneğinde Aleviler, Poşalar, Ahıska Türkleri ve Afganların kentsel yaşamdaki mekânsal, sosyal ve sınıfsal durumlarını tartışmaktadır.
Analysis of CSN2 Exon 7 gene diversity and its association with Sapera goat milk composition
Authors: Deviandini G. , Noor R. R. , Taufik E. , Asmarasari S. A.
Published: August 2023

Sapera goats, a crossbreed between Saanen and Etawa Grade goats, are known for their milk pro-duction and adaptability to the Indonesian climate. Genetic selection is necessary to improve the Sa-pera goat's milk quality and quantity. CSN2 gene is one of the genes that can affect the composition of goat milk. Therefore, this study aimed to identify the mutation points (SNPs) at the CSN2 exon 7 gene and its association with Sapera goat's milk composition. This study used sixty-six blood and milk sam-ples of Sapera goats collected from the Livestock Research Center, Ciawi. This study found two muta-tion points (SNPs) at g.8946C>T and g.8956G>A. The association analysis showed that SNP g.8946C>T was significantly associated with the lactose and salt content of Sapera goat's milk. In con-clusion, SNP g.8946C>T can be used as a genetic marker to improve the composition of Sapera goat milk with high lactose and salt content.
A study on the evaluation of Indonesian local microbial phytase supplementation and its impact on broiler chicken performance, metabolic energy utilization, ileal histomorphology, and meat and bone mineralization
Authors: Suryani A. E. , Anggraeni A. S. , Karimy M. F. , Istiqomah L. , Herdian H.
Published: August 2023

The effect of local Indonesian microbial phytase, derived from L. plantarum A1-E extracted from the small intestine of Indonesian free-range chickens and C. tropicalis TKd-3 obtained from fermented Indonesian soybean tempe, on growth performance, metabolizable energy, ileal histomorphology, and meat and bone mineralization in broilers is the subject of evaluation in this study. A total of 140 day-old broiler chicks were divided into four treatments, five replicates, and seven chicks in each replicate. The research was carried out for 28 days. The experimental groups consisted of a basal diet without phytase (FA) serving as the negative control, a basal diet supplemented with 500 FTU/kg of L. plantarum A1-E phytase (FB), a basal diet supplemented with 500 FTU/kg of C. tropicalis TKd-3 phytase (FC), and a basal diet with commercial phytase as the positive control (FD). The results revealed that the broiler performance index increased by the FB and FC treatments (P<0.05), and the FC treatment exhibited a tendency to enhance metabolizable energy (P=0.06). The result of ileum histomorphology showed that the FB treatment increased villous height, the ratio of villous height to crypt depth, and villous surface area (P<0.05). The highest mineral content of phosphorus (P), magnesium (Mg), and iron (Fe) in broiler breast meat was obtained in the FB treatment (P<0.05). Furthermore, the highest mineral content of calcium (Ca), P, Mg, zinc (Zn), and Fe in thigh meat was found in the FC treatment (P<0.05). The mineralization of the tibia bone demonstrated that the FB treatment exerted a significant effect (P<0.05) on the P, Mg, and Fe mineral content. In conclusion, the performance index increased due to microbial phytase supplementation. Besides that, L. plantarum A1-E phytase improved the surface area of the villus, the absorption of ileum minerals, the mineral content of breast meat, and minerals deposition in the tibia.
Evaluasi Prolanis Hipertensi Berbasis Teori Chain of Events di Puskesmas Playen II
Authors: Fatasyadhuha Nufika , Jati Sutopo Patria , Nandini Nurhasmadiar
Published: August 2023

According to the 2018 Riskesdas data, hypertension is a non-communicable disease with a significant increase in trend. Considering this case, hypertension has an urgency to be treated because it is a risk factor for the highest cause of death and disability according to the Institute for Health Metrics and Evaluation 2019. In this case Prolanis can be used as an alternative treatment for hypertension because it meets the criteria for the benefits of non-pharmacological therapy. We chose Puskesmas Playen II in Gunungkidul Regency as our research site because the coverage of providing hypertension services through Prolanis is still low, considering from the secondary data from the Gunungkidul Regency Health Office in 2020 where the percentage of hypertension services at the Puskesmas Playen II was only 0.3%, far below the 29 other puskesmas in Gunungkidul Regency. Hence, the study aimed to evaluate Prolanis' achievements in hypertension in Puskesmas Playen II using the Chain of Events evaluation theory. This research is qualitative with an evaluation study design. There were 15 informants as research subjects selected by purposive sampling. Prolanis' achievements in hypertension at Puskesmas Playen II have not reached the minimum target of 5% based on BPJS Kesehatan Regulation Number 7 of 2019, because the number of Prolanis-registered hypertensive patients is increasing but not matched with the increasing number of controlled hypertension sufferers. The Prolanis component also influences its unattainable achievement in follow-up treatment, so it is necessary to conduct routine monitoring of Prolanis participants’ lifestyles.
Pengaruh Gaya Kepemimpinan terhadap Kinerja Perawat dalam Keselamatan Pasien Di Unit Rawat Inap RSUD Penembahan Senopati
Authors: Fitriana Desi , Mahfud Mahfud , Putri Imram Radne Rimba , Fatimah Fatma Siti
Published: August 2023

Rumah sakit dalam memberikan pelayanan kesehatan harus mengutamakan keselamatan nyawa pasien. Ketidaktahuan tentang patient safety menimbulkan kerugian bagi pasien dan pihak rumah sakit sehingga peran pimpinan sangat penting untuk meningkatkan patient safety melalui kinerja perawat. Tujuan penelitian ini adalah untuk mengetahui pengaruh gaya kepemimpinan dan kinerja perawat terhadap keselamatan pasien di unit rawat inap RS Penembah Senopati. Penelitian ini menggunakan metode penelitian analitik kuantitatif dengan desain cross sectional. Sampel penelitian ini adalah 128 perawat di unit rawat inap RS Penembahan Senopati. Pengambilan sampel menggunakan teknik sample random sampling dengan pengumpulan data menggunakan kuesioner.Hasil uji statistik koefisien korelasi adalah 0,408 yang berarti menunjukkan korelasi sedang. Hasil uji Kendall Tau diperoleh nilai p sebesar 0,000 (p<0,05). Hasil penelitian menunjukkan bahwa variabel gaya kepemimpinan dalam kategori baik berpengaruh terhadap kinerja perawat dalam kategori baik sebanyak 95 (74,2%) responden. Dengan demikian hasil penelitian menunjukkan bahwa terdapat pengaruh gaya kepemimpinan terhadap kinerja perawat dalam keselamatan pasien rawat inap.
Physiological and AI-based study of endophytes on medicinal plants: A mini review
Authors: Kunwar Saloni , Joshi Aditya , Gururani Prateek , Pandey Deepa , Pandey Neha
Published: August 2023

Research on the natural resources found in medicinal plants and endophytes makes important contributions to a wide variety of fields, including drug development, agribusiness, biotechnology, and sustainable development. Endophytes are a group of microorganisms that can be discovered in the rhizosphere of plants being used in medical treatment. These microorganisms have the capability of producing a wide range of primary and secondary metabolites by utilizing a variety of distinct biosynthetic pathways. Several different technologies, such as genetic modification and artificial intelligence (AI), play a significant role in the acceleration of endophytic research. These methods aid in the discovery and synthesis of novel compounds with medicinal promise, the predictive analysis of bioactive compounds, the identification and classification of endophytes, and the optimization of potential bioactive compounds. In light of this, the current review focuses on providing a concise comprehension of the influence of bioactive compounds secreted by specific endophytes on medicinal plants through the application of significant technologies in the field of endophytic research.
Impact of simultaneous Cd and Zn substrate amendments on metal accumulation in two Cd/ Zn hyperaccumulating Thlaspi species
Authors: Pongrac Paula , Brvar Eva , Regvar Marjana
Published: August 2023

The impact of simultaneous Cd and Zn amendments in the substrate on the accumulation of Cd and Zn were studied in a recently discovered Cd/ Zn hyperaccumulating Thlaspi praecox (Brassicaceae) and compared to a model hyperaccumulating plant species T. caerulescens. The plants were grown in pots with added Cd or Zn or both for three months in a greenhouse. The addition of Zn in the substrate increased Cd extractability in the substrate significantly without a significant pH change and this increase resulted in increased concentration and content of Cd in the shoots of both species indicating that species have similar abilities to extract Cd from the substrate. In the combined treatment (Cd and Zn) an increase in shoot biomass accompanied with a decrease in Zn concentration in roots and shoots of both species was observed, while no changes in total accumulated Zn in shoots were seen. These results suggest different uptake and translocation systems for Cd and Zn in T. praecox, positioning this plant species in the superior Cd hyperaccumulating league of T. caerulescens Ganges ecotype.
Growth-dependent and adaptive mutation rates to ebgR and IS30 transposition in the bacteria Escherichia coli K-12 at different extracellular Mg2+ concentrations
Authors: Krašovec Rok , Jerman Igor , Jan Luka
Published: August 2023

During starvation on carbon and energy Escherichia coli K-12 cells, modified to possess EbgA51 as the only く-galactosidase enzyme, experience adaptive mutations in the ebgR repressor gene. In this way, cells acquire the capacity to utilize the lactulose as the only source of carbon and energy and begin to grow. Adaptive mutations at ebgR are mediated largely by insertion sequences, 40% of adaptive mutants contain IS30 insertions. Also, besides sensing extracellular Mg2+, a PhoP-PhoQ system decreases the adaptive mutation rate to ebgR in a to-date unknown way. By performing fluctuation tests and genetic analyses, we tested the hypothesis that Mg2+ plays an important role in the adaptive mutation at ebgR. Results gathered with phoP and phoQ mutant strains demonstrated that the adaptive, but not the growth-dependent, mutation rate is increased by a high extracellular Mg2+ concentration. In an Mg2+-rich environment, the phoQ cells experience a nearly identical adaptive mutation rate as the wild-type strain. Results with the wild-type strain show that the relation between the levels of PhoP-PhoQ expression and the adaptive mutation rate is not as straightforward as expected and that different Mg2+ concentrations do not affect IS30 transposition. We discuss the possible role of magnesium in the adaptive mutation process.
Vegetation of the depressions with Eleocharis quinqueflora in spring fens in Slovenia
Authors: Zelnik Igor , Martinčič Andrej , Vreš Branko
Published: August 2023

During the investigations of wetlands in Slovenia over the last decade speciﬁc plant communities in spring fens were found. Stands with species Eleocharis quinqueﬂora occurring in depressions inundated with standing and/or running water were found in the Alpine, pre-Alpine and Dinaric phytogeographic regions of Slovenia. Standard Central European method for vegetation research was used and multivariate analyses were performed using Syn-Tax program. Stands were classiﬁed in two different species-poor, small-scale plant communities, most of them into associationEleocharitetum pauciﬂorae Lüdi 1921. This rare plant community occurs in the Alpine and Carpathian regions and in northern Europe and has not been recorded in Slovenia before. The association Eleocharitetum pauciﬂorae is a two-layered plant community of calcium-rich fens. It thrives in shallow temporary paddies and on the sandy or stony slopes with seeping water. Smaller group of relevés was classiﬁed into association Scorpidio-Utricularietumminoris Ilschner ex T.Müller et Görs 1960. This association thrives in permanent paddies, where the water is deeper as in a case of the ﬁrst association. Since the dominating species Eleocharis quinqueﬂora and Utricularia minor, respectively, have the status of a vulnerable species according to Red List of Slovenia, the stands of the studied communities, which represent vital populations, should be preserved as well as the corresponding habitat types.
Biological knowledge of Slovenian students in the living systems content area in PISA 2006
Authors: Strgar Jelka
Published: August 2023

In the PISA 2006 program Slovenian students exceeded the international average of scientiﬁc achievements, while in the ﬁeld of living systems performedbelow the national average. The purpose of our analysis was to determine in which areas of biology Slovenian students are weak and in which they arestrong, and to determine their biological knowledge in comparison to 23 other European countries, which, by various criteria, are the most comparable to Slovenia and interesting for us. We analysed 24 tasks that tested the biological knowledge in PISA 2006. We found that student achievement in Slovenia wasn’t poor in any of the tested biological topics. There are two factors that can explain lower achievements resolving individual tasks: (1) Question type: Students poorly answered the open-constructed response questions that require independent formulation of coherent responses. They were more successful with complex multiple choice questions and multiple choice questions, where they had to choose the correct answer from several given suggestions; (2) Difﬁculty: Students didn’t perform as well with higher cognitive level tasks which required using knowledge. This suggests that in the period when these students were receiving elementary education, their biology teaching focused on developing knowledge of biological content (knowledge of science) and competence explaining phenomena scientiﬁcally, while the development of other knowledge (knowledge about science) and the competence to draw evidence-based conclusions (using knowledge) was inadequate. It is therefore in the hands of all in Slovenia involved in biological education, especially teachers of biology, to give students more opportunities for problem-solving, rather than only focusing on content.
Mentorju, akademiku in profesorju, dr. Zvonimirju Devidéju – ob 90. obletnici rojstva in 64. letnici dela v znanosti in izobraževanju
Authors: Krajnčič Božidar
Published: August 2023

Prispevek je namenjen mojemu vodniku in mentorju, profesorju dr. Zvonimirju Devidéju, priznanemu biologu in botaniku, članu Hrvaške akademije znanosti in umetnosti, profesorju na Naravoslovno – matematični fakulteti Univerze v Zagrebu, ob 90. obletnici rojstva in 64. letnici dela v znanosti in izobraževanju.
The importance of education of future elementary teachers about modern biotechnology issues
Authors: Ambrožič Dolinšek Jana , Šorgo Andrej
Published: August 2023

The tremendous development of science and technology has influenced many aspects of our everyday lives, society and environment. A good example of such technology is biotechnology. However, besides its promise, this technology has alsoraised several controversial issues to which answers are not easily available. With increasing knowledge and applications on one side and controversy on the other the teaching of science is, anything but easy. Development of competencies for these issues, and questions like why, when, and how to integrate modern biotechnology into science education are becoming prominent in the near future. Nowadays, when we are confronted with issues of varying degrees of complexity and importance, it is necessary that teachers at all levels of education have the basic tools to cope with these issues. This is one of reason why we have attempted to establish what kind of knowledge, values and opinions about genetic engineering and genetically modified organisms (GMOs) are characteristic for the students, future Elementary Teachers, at three Slovene Faculties of Education. We collected answers of 360 questionnaires from pre-service elementary school teachers and analysed their statements from the field of general and classical genetics, modern biotechnology, legislation and the acceptance of different kind of GMOs. Prospective teachers have some knowledge of general and classical genetics and less knowledge about the use of modern biotechnology. They have concerns and fears about different kind of GMOs, mostly negative attitudes towards different kinds of GMOs, or they hold no strong opinions about them. Microorganisms and plants are generally more acceptable than GM animal. Furthermore, more knowledge does not mean that individual GMOs are more acceptable.
Secondary structures of Potato spindle tuber viroid variants detected in Slovenia
Authors: Viršček Marn Mojca , Mavrič Pleško Irena , Gerič Stare Barbara
Published: August 2023

A survey of Potato spindle tuber viroid was initiated in Slovenia in 2006. Until the end of 2010, 100 samples of ornamental plants were found to be infected. Analysis of 96 complete master sequences of Slovene samples revealed new genetic variants. Our sequence variants showed considerable variation in their predicted secondary structure. The variability was observed in the pathogenic, central and variable domains. In several cases even single mutations were sufficient for the change in predicted secondary structure.
Medicinal mushrooms native to Slovenia
Authors: Gregori Andrej
Published: August 2023

Slovenia with its diverse environment is home to more than 2400 fungal species out of which especially many macromycetes have for millennia been used worldwide as natural remedies. These species of mushrooms were in the past picked from the nature, but today can be cultivated as fruiting bodies or fungal biomass on different substrates. They possess immunomodulating, antiviral, antibacterial and anticancer activities and can be used against allergies, dementia, Alzheimer disease and in many other diseases. They represent a vast potential as natural remedies with no or very little adverse effects and can be processed into food supplement or further developed into medicines. These mushrooms are a natural treasure, which enables us to be more self-sufficient if we cultivate them for medical and certain species for nutritional purposes as well.
Methodological weaknesses regarding the use of PEF-meters when assessing physiological effects of air pollution risk factors
Authors: Golja Petra
Published: August 2023

The manuscript discusses the applicability of self-administered monitoring of respiratory function in asthma patients in order to discern between negative health effects of different air pollutants on respiratory function. Thirteen asthma patients measured their respiratory function twice daily over a one month winter period. They used PEF-meters to monitor peak expiratory flow (PEF;L/min) and forced expiratory volume in the first second of expiration (FEV1; L). Subjects’ vital capacity (VC; L) was measured in a laboratory setting at the end of the measuring period. Respiratory data were evaluated in respect to the ambient concentrations of NO2, NOx, SO2, O3, and PM10, which were monitored during the same period. The concentrations of some air pollutants (PM10 and NOx) exceeded the critical levels on several days during the study. PEF-meter data (PEF, FEV1 and FEV1/VC), however, did not respond significantly to the ambient conditions (P>0.05). The results speak against the use of self-administered PEF-meter monitoring for the recognition and investigation of air pollutant related negative health effects. Limitations and delimitations of the method are presented.
Presence and abundance of macrophytes in Lake Slivniško jezero
Authors: Golob Aleksandra , Gaberščik Alenka , Germ Mateja
Published: August 2023

Macrophytes are an important part of the lake biota. They are also bioindicators of environmental conditions. The goal of the present research was to determine species richness and abundance as well as longitudinal and depth distribution of macrophytes in Lake Slivniško jezero. A survey of macrophytes in the whole lake littoral was made, the minimum and maximum depth of taxa were measured and their abundance was estimated as well. We also assessed selected environmental parameters of the littoral and catchment. 22 macrophyte taxa: 9 emergent, 9 submerged and 4 natant macrophytes were determined. The most frequent species were Phragmites australis, Najas marina, Myriophyllum spicatum and Potamogeton nodosus. The maximum depth of colonisation was achieved by Nymphaea alba (to 2.4 m), while M. spicatum and N. marina grown to the depth of 1.9 m. According to CCA the distribution of macrophytes was significantly influenced by exposition, bottom slope, sediment type, slope of riparian zone, macroalgae abundance, type of riparian vegetation, completeness of riparian zone, land-use beyond the riparian zone and water turbidity.
Phytosociological description of hay meadows with dominating Trisetum flavescens in the lower montane belt of north-western and western Slovenia
Authors: Dakskobler Igor , Seliškar Andrej
Published: August 2023

We conducted a phytosociological study into hay meadows on former fields on original sites of beech forests form the alliance Aremonio-Fagion in the lower montane belt of the northwestern and western Slovenia (southern Julian Alps, northern part of the Dinaric Alps) and compared them to similar, previously described meadows in Slovenia and northwestern Italy. Based on this comparison they are classified into the new association Rhinantho freynii-Trisetetum flavescentis and new habitat type, southeastern-Alpine-northern-Illyrian lower montane hay meadows – 38.239-S1.
Extraction of DNA from different sample types – a practical approach for GMO testing
Authors: Žel Jana , Demšar Tina , Štebih Dejan , Milavec Mojca , Gruden Kristina
Published: August 2023

Current methods based on DNA targets for the detection, identification and quantification of genetically modified organisms (GMOs) involve extraction of the DNA. Different extraction procedures have been developed for the great variety of samples from food, feed, seeds and particular plant parts. This makes the operation of routine analytical laboratories complex and workloads heavy. Here we present a decision-making system, developed over many years of GMO testing on different samples, that result in the application of only a few extraction methods for the majority of samples. Developed decision-making system enables quicker and more cost effective testing of GMOs. In addition, the performance of DNA extraction resulting from the use of the selected extraction methods is presented for use in subsequent testing of GMOs by real time PCR methods. This approach can be used as a model for similar systems based on nucleic acid analysis in food, feed, seeds and plants.
16. mednarodni simpozij mikrobne ekologije - ISME 16
Authors: Mulec Janez
Published: August 2023

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Herbarij v izobraževanju učiteljev razrednega pouka
Authors: Torkar Gregor , Mavrič Irma
Published: August 2023

V članku opredeljujemo pomen herbarija v izobraževanju učiteljev razrednega pouka za dvig interesa za rastline in znanja o rastlinah med osnovnošolci. Slovenske študente, bodoče učitelje razrednega pouka, smo usposabljali v izdelovanju herbarija. V raziskavi je sodelovalo 86 študentov. Študenti so po izdelavi herbarijev izpolnili anketni vprašalnik, s katerim smo preverjali njihovo znanje in izkušnje. Študenti poročajo, da so med izdelovanjem herbarijev v povprečju spoznali tri nove rastlinske vrste. 74% študentov je imelo ob tem težave, predvsem z določanjem vrst in postopki herbariziranja. Več kot dve tretjini študentov je herbarij izdelovalo že vosnovni ali srednji šoli, predvsem pri predmetih naravoslovje in biologija. Vsi, z izjemo enega študenta, nameravajo herbarij v bodoče vključiti v pouk. V aktivnosti vidijo predvsem potencial za poučevanje učencev o anatomiji in vrstni pestrosti rastlin, zarazvijanje njihovih naravoslovnih spretnosti ter odnosa do dela in biologije. Sklenemo lahko, da so sodelujoči študenti prikazali ustrezen nivo poznavanja vsebine ter znanja za poučevanje za uspešno implementacijo herbarija v šolo, kar lahko vodi do dvigainteresa za rastline in znanja o rastlinah med osnovnošolci.