bluuebunny/crossref_metadata_2025 huggingface dataset:
Acompanhamento fonoaudiológico do aleitamento materno em recém-nascidos nas primeiras horas de vida
Authors: Medeiros Andréa Monteiro Correia , Santos Jéssica Caroline de Jesus , Santos Daniela de Arimatéia Rosa , Barreto Ikaro Daniel de Carvalho , Alves Yasmin Vieira Teixeira
Published: November 2017

RESUMO Introdução A fonoaudiologia tem assumido importante papel junto ao aleitamento materno. Objetivo Verificar a situação do aleitamento materno, considerando aspectos da díade mãe-recém-nascido, de acordo com tempo de vida do recém-nascido, mediante intervenção fonoaudiológica. Métodos Estudo realizado de maio de 2015 a setembro de 2016, com 166 díades mãe-recém-nascido, durante a situação do aleitamento materno, em uma maternidade pública de alto risco do estado de Sergipe. Foi utilizado protocolo específico, registrando-se aspectos anatômicos de bicos e mamas maternos, estado comportamental, padrão de pega e sucção dos recém-nascidos e postura das díades, para descrever a situação de aleitamento materno, nos três diferentes momentos: Momento 1 (linha de base); Momento 2 (intervenção fonoaudiológica); Momento 3 (acompanhamento). As análises foram descritas por meio de frequências simples e percentuais, utilizando o teste de Mantel-Haenzsel (M-H) e o teste da razão de verossimilhança (TRV), adotando nível de significância de 5%. Resultados Em relação ao efeito da intervenção fonoaudiológica, houve diferença significativa quanto aos parâmetros maternos, para situação das mamas (ingurgitamento). Nos parâmetros envolvendo os recém-nascidos, houve diferenças significativas, com melhora no padrão de pega, quanto aos seguintes aspectos: não abocanha somente o bico, abocanha parte da aréola, pega efetiva e consegue manter a pega. Quanto aos aspectos posturais da díade, houve diferenças significativas quanto à cabeça do recém-nascido elevada e alinhada e ao contato corporal (“barriga com barriga”). Conclusão A intervenção fonoaudiológica junto às díades mães-recém-nascidos, logo nas primeiras horas pós-parto, evidenciou melhora nos parâmetros considerados fundamentais para o sucesso da amamentação, vislumbrando uma prática de educação em saúde junto às puérperas.
Correlations between spectral and biophysical data obtained in canola canopy cultivated in the subtropical region of Brazil
Authors: Pinto Daniele Gutterres , Fontana Denise Cybis , Dalmago Genei Antonio , Fochesatto Elizandro , Vicari Matheus Boni , Bremm Carolina , Cunha Gilberto Rocca da , Gouvêa Jorge Alberto de , Santi Anderson
Published: November 2017

Abstract: The objective of this work was to identify the spectral bands, vegetation indices, and periods of the canola crop season in which the correlation between spectral data and biophysical indicators (total shoot dry matter and grain yield) is most significant. The experiment was carried out during the 2013 and 2014 crop seasons at Embrapa Trigo, in the state of Rio Grande do Sul, Brazil. A randomized complete block design was used, with four replicates, and the treatments consisted of five doses of nitrogen topdressing. Plant dry matter, grain yield, and phenology were measured. The canola spectral response was evaluated by measuring the canola canopy reflectance using a spectroradiometer, and, with this data, the SR, NDVI, EVI, SAVI, and GNDVI vegetation indices were determined. Pearson’s correlations between the spectral and biophysical variables of canola showed that the red (620 to 670 nm) and near-infrared (841 to 876 nm) bands were the best to estimate the dry matter. The vegetative period is the most indicated to obtain the most significant correlations for canola. All the used vegetation indices are adequate for estimating the dry matter and grain yield of canola.
Economic analysis of sorghum consortia with forages or with dwarf pigeon pea succeeded by soybean or corn
Authors: Pascoaloto Isabô Melina , Andreotti Marcelo , Cruz Sanderley Simões da , Sabbag Omar Jorge , Borghi Emerson , Lima Gilmar Cotrin de , Modesto Viviane Cristina
Published: November 2017

Abstract: The objective of this work was to determine the most economically viable integrated no-tillage crop-livestock system by intercropping sorghum with tropical forages or dwarf pigeon pea, succeeded by soybean or corn, over a two-year period. The experiment was carried out in a randomized complete block design with four replicates. The treatments consisted of forage sorghum intercropped with: 'Marandu' grass; 'Marandu' grass and dwarf pigeon pea; 'Mombaça' grass; 'Mombaça' grass and dwarf pigeon pea; and dwarf pigeon pea and single sorghum, harvested for silage in the first cut and regrowth, succeeded by soybean or corn. The following calculations were made: effective operational cost, total operational costs, gross revenues, operational income, profitability index, equilibrium price, and equilibrium productivity. These variables were determined for individual crops, as well as for the sum of the crops occupying the same area over time. The sorghum consortia with 'Mombaça' grass, with or without dwarf pigeon pea, had 18 and 14% lower productivity and profitability, respectively, than the sorghum consortia with 'Marandu' grass, with or without dwarf pigeon pea. Soybean in succession requires less chemical control of the 'Mombaça' grass than corn in succession.
Phytochemical profile of cansanção nettle extracts and their bioactivities on cabbage caterpillar
Authors: Carvalho Neto Moisés Felix de , Gervásio Rita de Cássia Rodrigues Gonçalves , Araújo Edigênia Cavalcante da Cruz , Almeida Jadson Cardoso de , Guimarães Amanda Leite
Published: November 2017

Abstract: The objective of this work was to evaluate the phytochemical profile and bioactivity of extracts of Brazilian stinging (“cansanção”) nettle (Cnidoscolus urens) on cabbage caterpillar (Ascia monuste orseis). Mortality, larval stage duration, pupal viability and mass, percentage of deformed pupae, and feeding preference were evaluated. The constituent classes of the ethanolic extracts were also identified by analytical thin-layer chromatography (TLC). Ethanolic extracts of leaves and roots at 2% (m/v) and aqueous extract of roots at 20% (m/v) prolonged the larval phase of the insect. The ethanolic extract of leaves at 2% reduced pupal viability, and pupae from all treatments showed reduced masses and generated deformed adults. Except for the ethanolic extract of stem at 2% (m/v), all other extracts showed a deterrent effect on A. monuste orseis larvae. The phytochemical analysis of ethanolic extracts showed the major presence of phenolic compounds, naphthoquinones, anthraquinones, coumarins, anthracene derivatives, terpenes, and steroids, as well as tannins. Aqueous extracts at 20% concentration and ethanolic extracts at 2% of leaves, stems, and roots of cansanção nettle cause larval mortality and also reduce leaf consumption, pupal viability, and pupal mass of A. monuste orseis.
Morphoagronomic and molecular characterization of ornamental passion fruit cultivars
Authors: Fonseca Kenia Gracielle da , Faleiro Fábio Gelape , Junqueira Nilton Tadeu Vilela , Barth Mariana , Feldberg Nelson Pires
Published: November 2017

Abstract: The objective of this work was to validate the morphoagronomic descriptors used in the protection processes of plant cultivars in Brazil, by characterizing six cultivars of ornamental passion fruit. The BRS Rubiflora, BRS Rosea Púrpura, BRS Céu do Cerrado, BRS Roseflora, BRS Estrela do Cerrado, and BRS Pérola do Cerrado cultivars, 33 morphoagronomic descriptors, and two molecular markers were used. The categorical morphoagronomic descriptors were analyzed by frequency distribution and multivariate analyses. The quantitative morphoagronomic descriptors were subjected to the analysis of variance and to the comparison of the means of each cultivar. The random amplified polymorphic DNA (RAPD) and the inter-simple sequence repeats (ISSR) markers were used for molecular analysis. A high-validation rate was observed for the morphoagronomic descriptors used in the protection of plant cultivars. The analyses of variance showed significant differences between the quantitative descriptors, and the molecular markers confirmed the genetic differences among the cultivars. There was a high correlation between the calculated distances based on the categorical morphoagronomic descriptors and molecular markers. The morphoagronomic descriptors and molecular markers are useful and complementary for the characterization and differentiation of cultivars.
Straw production and agronomic performance of soybean intercropped with forage species in no-tillage system
Authors: Andrade Carlos Augusto Oliveira de , Borghi Emerson , Bortolon Leandro , Bortolon Elisandra Solange Oliveira , Camargo Francelino Peteno de , Avanzi Junior Cesar , Simon Jones , Silva Rubens Ribeiro da , Fidelis Rodrigo Ribeiro
Published: November 2017

Abstract: The objective of this work was to evaluate the straw production and the agronomic performance of soybean intercropped with oversown forage species, in no-tillage system. A randomized complete block design was carried out with four replicates, in a 5×2+2 factorial arrangement, as follows: five forage species - Urochloa brizantha 'Marandu', Urochloa ruziziensis, Panicum maximum 'Mombaça', Panicum maximum 'Massai', and Pennisetum americanum -, intercropped with soybean over two crop years (2013/2014 and 2014/2015), plus two controls, with P. americanum sowed in succession to soybean or with soybean monocropping followed by winter fallow (traditional cultivation). Soybean yield components and forage straw yield were evaluated. None of the intercropping systems reduced soybean grain yield, compared with monocropped soybean. The oversown species can significantly improve soybean productivity, as is the case for soybean intercropped with P.maximum 'Mombaça', compared with soybean monocropping, followed or not by millet. Panicum maximum 'Mombaça' is the most effective forage species for dry matter accumulation in the fall/spring period.
Agronomic performance of 'Cabernet Sauvignon' with leaf removal management in a high-altitude region of Southern Brazil
Authors: Würz Douglas André , Brighenti Alberto Fontanella , Marcon Filho José Luiz , Allebrandt Ricardo , Bem Betina Pereira de , Rufato Leo , Kretzschmar Aike Anneliese
Published: November 2017

Abstract: The objective of this work was to compare the effect of different times of leaf removal on the vegetative-productive efficiency and on the chemical and physical characteristics of 'Cabernet Sauvignon' clusters in a high-altitude region of the state of Santa Catarina, Brazil. The experiment was conducted in the 2015 and 2016 vintages, in a commercial vineyard, located in the municipality of São Joaquim, in the state of Santa Catarina, at 1,230-m altitude. The treatments consisted of different times leaf removal in the cluster zone, in the following phenological stages: full bloom, buckshot berries, pea-sized berries, veraison, and 15 days after veraison, besides a control without leaf removal. The evaluated variables were subjected to the analysis of variance and compared with the Scott-Knott test, at 5% probability. In a high-altitude region, leaf removal provides more adequate indices for plant vegetative:yield balance. It also increases yield when performed during the pea-sized berry and veraison stages, increases the fertility index when performed during the pea-sized berry stage, and improves the chemical composition of 'Cabernet Sauvignon' grape when performed up to the pea-sized berry stage. The obtained results show the importance of early leaf removal to obtain must with potential for the production of quality wines.
Anatomic compatibility of pear and quince trees grafted on Pyrus calleryana and Chaenomeles sinensis rootstocks
Authors: Melo Evaldo Tadeu de , Pio Rafael , Balbi Rodrigo Vieira , Ferreira Cassiana Alves , Mori Fábio Akira
Published: November 2017

Abstract: The objective of this work was to evaluate the graft growth and the anatomical characterization of pear (Pyrus communis x P. pyrifolia) and quince (Cydonia oblonga) cultivars grafted on Pyrus calleryana and Chaenomeles sinensis rootstocks. Sprouted graft percentages at 60 days and the length and diameter of the graft sproutings at 120 days were evaluated. Stem cuttings from the grafting region were performed in transversal and longitudinal sections, and the secondary xylem was separated for tissue comparison. The pear trees showed good development on P.calleryana rootstock, and the anatomical sections showed that the vascular tissues were connected, which did not occur when the trees were grafted on C.sinensis, in which the grafts sprouted, but did not develop, and the vascular tissues did not connect. The quince trees showed good development on both rootstocks due to the good connection of the vascular tissues. The connection of vascular tissues is essential for the success of grafting. The evaluated species did not differ significantly as to their secondary xylem tissues. The incompatibility found between the pear trees and the C.sinensis rootstocks is not anatomical.
Prediction model for phenology of grapevine cultivars with hot water treatment
Authors: Lerin Sabrina , Grohs Daniel Santos , Almança Marcus André Kurtz , Botton Marcos , Mello-Farias Paulo , Fachinello José Carlos
Published: November 2017

Abstract: The objective of this work was to prepare a prediction model for the phenology of grapevine cultivars (Bordô, Cabernet Sauvignon, Moscato Embrapa, Paulsen 1103, SO4, and IAC 572) using hot water treatment. The heat treatment with hot water consisted of combinations of three temperatures (50, 53, and 55°C) and three time periods (30, 45, and 60 min), with or without previous hydration for 30 min. After the treatments, the cuttings were planted in the field and their phenological development was evaluated during two months. The six studied cultivars presented different responses to the effects of the factors temperature and time, but did not differ significantly regarding hydration. It was possible to develop a mathematical model for the use of hot water treatment in grapevine cuttings, based on phenological development ( y phenology = 48.268 − 0.811 x 1 − 0.058 x 2) and validated by the variables sprouting and root emission. From the developed model, it is recommended that the hot water treatment be applied in the temperature range between 48 and 51°C for cuttings of all cultivars.
Genome-wide association for mapping QTLs linked to protein and oil contents in soybean
Authors: Dias Douglas Antônio , Polo Leandra Regina Texeira , Lazzari Fabiane , Silva Glacy Jaqueline da , Schuster Ivan
Published: November 2017

Abstract: The objective of this work was to identify single-nucleotide polymorphism (SNP) markers linked with quantitative trait loci (QTLs) associated with increased contents of protein and oil in soybean. A total of 169 Brazilian soybean varieties, genotyped with 6,000 SNP markers, were evaluated. Protein and oil contents were obtained with the near-infrared reflectance method. Correlation and multiple linear regression analyses were used to identify linkage disequilibrium between SNP markers and the QTLs associated with the two characteristics. Seven QTLs were found to be associated with protein content, on six chromosomes (2, 6, 11, 12, 13, and 16), explaining 60.9% of the variation in this trait. For oil content, eight QTLs were identified on six chromosomes (1, 4, 5, 6, 17, and 19), explaining 78.3% of the variation in the trait. The correlation between the number of loci containing favorable alleles and the evaluated characteristics was 0.49 for protein content and 0.60 for oil content. The molecular markers identified are mapped in genomic regions containing QTLs previously mapped for both characteristics, which reinforces the association between these regions and the genetic control of oil and protein contents in soybean.
Meat quality and performance of pigs fed diets with fish silage meal
Authors: Cândido Rayssa Santos , Watanabe Pedro Henrique , Oliveira Paula Joyce Delmiro de , Angelim Alysson Lira , Siqueira André de Freitas , Ximenes Julio César Martins , Normando Leonardo Ribeiro Oliveira , Melo Juliana Mendes , Freitas Ednardo Rodrigues
Published: November 2017

Abstract: The objective of this work was to determine the nutrient digestibility and metabolizable energy (ME) of fish silage, as well as to evaluate the effect of the dietary inclusion of fish silage meal (FSM) in diets on the performance, carcass characteristics, meat quality, sensory analysis of meat and mortadella, and economic viability of growing and finishing pigs. In the digestibility assay, 16 barrows (33.20±4.93 kg) received diets with and without FSM. The fish silage had 39.01% crude protein and 4,032 kcal kg-1 ME. In the performance assay, 32 barrows (26.00±1.68 kg) were fed diets containing different inclusion levels of FSM (0, 25, 50, and 75%). FSM, obtained from the mixture (1:1) of fish silage with corn, showed a quadratic effect on average daily gain, and the best result was obtained with the inclusion level of 25.83%. The results for feed conversion and economic viability indicate that up to 25% FSM, corresponding to 5.87% of fish silage based on dry matter, can be used in the pig growing and finishing phases.
Aluminum species and activity in sandy soil solution with pig slurry addition
Authors: Conti Lessandro De , Ceretta Carlos Alberto , Couto Rafael da Rosa , Ferreira Paulo Ademar Avelar , Silva Lincon Oliveira Stefanello da , Piccin Rogério , Lourenzi Cledimar Rogério , Girotto Eduardo , Brunetto Gustavo
Published: November 2017

Abstract: The objective of this work was to evaluate the effect of pig slurry applications on the exchangeable Al content, activity, and distribution of Al chemical species in the soil solution. Soil samples of a Typic Hapludalf were obtained from an experiment conducted during eight years, with 19 applications of 0, 20, 40, and 80 m3 ha-1 pig slurry. The soil samples were collected in stratified layers to determine exchangeable Al content and to extract the soil solution by the saturated soil paste method. The concentrations of the main cations and anions, dissolved organic carbon, and pH in the soil solution were determined. The distribution of the chemical species of Al and the activity of the Al3+ species were obtained through the Visual Minteq software. The application of pig slurry does not change the exchangeable Al content in the soil and the Al3+ activity in the soil solution. The low Al3+ activity in the topsoil layers does not present risk of toxicity for most crops. Pig slurry applications increase the proportion of the AlHPO4 + and AlSO4 + species in the soil solution, by increasing the concentration of soluble inorganic ligands.
Magnetic and spectral signatures of Cerrado soils in the state of Goiás, Brazil
Authors: Fernandes Kathleen Lourenço , Ribon Adriana Aparecida , Marques Junior José , Bahia Angélica Santos Rabelo de Souza , Tavares Filho João
Published: November 2017

Abstract: The objective of this work was to estimate the iron oxide contents (hematite and goethite) and to characterize the color and the spectral and magnetic signatures of Cerrado soils in the state of Goiás, Brazil. Six Oxisols and one Inceptisol were studied. Spectral and magnetic signatures were determined by diffuse reflectance spectroscopy (DRS) and magnetic susceptibility, respectively. Then, the spectral curves and the second derivative calculations were used to determine hematite and goethite contents, as well as soil color after conversion into tristimulus values. Hematite and goethite contents were also obtained by x-ray diffractometry, and soil color was also defined in the field (Munsell color chart). The values for the isomorphic substitution of iron by aluminum and the degree of redness were also determined. DRS can be used to estimate hematite and goethite contents, as well as the color of Cerrado soils in the state of Goiás. The spectral signature can point out the main soil properties related to the contents of organic matter, iron oxides, kaolinite, and gibbsite. The magnetic signature, characteristic of soils rich in iron oxides (hematite and goethite), shows the predominance of pedogenic minerals.
Composition, content of bioactive compounds, and antioxidant activity of fruit pulps from the Brazilian Amazon biome
Authors: Virgolin Lara Borghi , Seixas Fernanda Rosan Fortunato , Janzantti Natália Soares
Published: November 2017

Abstract: The objective of this work was to evaluate the physicochemical composition, the bioactive compounds, and the total antioxidant activity of the fruit pulps of abiu (Pouteria caimito), achachairu (Garcinia humilis), araza (Eugenia stipitata), bilimbi (Averrhoa bilimbi), and yellow mangosteen (Garcinia xanthochymus) from the Brazilian Amazon biome. Total soluble solid content, total and reducing sugar contents, titratable acidity contents, pH, ascorbic acid content, moisture, protein, lipid, ash, and caloric value were determined. The contents of total anthocyanins, yellow flavonoids, and total carotenoids, besides total phenolic compounds and antioxidant activity, were also evaluated. The abiu pulp showed the highest contents of ash, total sugars, reducing sugars, total soluble solids, calorie value, and pH, among the analyzed fruits. The achachairu pulp showed the highest content of total phenolic compounds, with significant antioxidant activity, while the araza pulp had the highest total carotenoid content, and the yellow mangosteen, the highest yellow flavonoid content. The positive correlation between antioxidant activity and total phenolic compounds suggests that these compounds are the most important for determining antioxidant activity in the evaluated fruit pulps.
Association of the severity of tongue lesions in broiler chickens with the quality of corn in feed
Authors: Vaccari Isabel Cristina Mores , Duarte Vinicius , Rodrigues Denise Russi , Santos Fabiana Ramos dos , Minafra Cibele Silva
Published: November 2017

Abstract: The objective of this work was to determine the correlation of the physical attributes of corn and the mycotoxins present in its grains with the severity of tongue lesions in broiler breeder roosters. The occurrence of the aflatoxin, vomitoxin, zearalenone, T-2 toxin, and fumonisin mycotoxins was detected in the corn grains used in the feed formulation for breeder chickens. The percentages of moisture and broken grains were positively correlated (Pearson’s correlation) with the presence of aflatoxin. Milder lesions (grade 1) are positively correlated with aflatoxin and negatively correlated with fumonisin, and the most severe lesions (grade 3) are positively correlated with vomitoxin, fumonisin, and T-2 toxin.
Effect of shoot topping intensity on 'Cabernet Franc' grapevine maturity in high-altitude region
Authors: Würz Douglas André , Marcon Filho José Luiz , Brighenti Alberto Fontanella , Allebrandt Ricardo , Bem Betina Pereira de , Magro Maicon , Rufato Leo , Kretzschmar Aike Anneliese
Published: November 2017

Abstract: The objective of this work was to evaluate the chemical composition of 'Cabernet Franc' grape subjected to different levels of shoot topping. The experiment was conducted in the 2008 and 2009 vintages, and the levels of shoot topping consisted of: control, without topping; and reductions of 15, 30, and 45% in total leaf area. The effect of shoot topping intensity on the chemical composition of the 'Cabernet Franc' grape berries was observed. A reduction of 26% in the vegetative canopy through a moderate shoot topping increased the total polyphenol index and the anthocyanin content. Shoot topping is a practice that must be carried out with caution, since severe shoot topping, i.e., a reduction greater than 26% of the leaf area, may negatively impact grape quality by reducing the content of soluble solids and decreasing the total polyphenol index and the content of anthocyanins of 'Cabernet Franc' grape berries.
Nutritional value of elephant grass genotypes
Authors: Souza Paulo Henrique de , Faturi Cristian , Rodrigues Luiz Fernando de Souza , Silva Filho Ednaldo da , Rêgo Aníbal Coutinho do , Rodrigues Filho José Adérito
Published: November 2017

Abstract: The objective of this work was to assess the nutritive value of four elephant grass (Pennisetum purpureum) genotypes. The chemical composition, intake by sheep, and digestibility of different genotypes (G1, G2, G3, and G4) were evaluated. A dry matter (DM) digestibility assay was performed with total leftovers and feces collected from 20 sheep kept in metabolic cages. G3 had lower DM intake in grams per animal per day compared with G1 and G2, and it had greater digestibility, crude protein, neutral detergent fiber, and acid detergent fiber values. G1, G2, and G4 have the best nutritive values among the evaluated genotypes.
Pyoverdine use for the control of passion fruit bacterial blight
Authors: Ribeiro Carla Maria Cavalcanti , Souza Giovanni Ribeiro de , Schurt Daniel Augusto , Halfeld-Vieira Bernardo de Almeida
Published: November 2017

Abstract: The objective of this work was to evaluate the effect of a bacterial filtrate containing pyoverdine on the population dynamics of Xanthomonas axonopodis pv. passiflorae, and the severity of passion fruit bacterial blight. The treatments were: King’s B medium solution; King’s B medium solution with 2 μmol L-1 Fe2+ supplementation; and King’s B medium filtrate containing two concentrations of the siderophore pyoverdine produced by Pseudomonas sp. The filtrate containing pyoverdine at Abs363 = 0.231 (highest concentration) reduces the number of cells of X. axonopodis pv. passiflorae, and at Abs363= 0.115 and Abs = 0.231 significantly reduces the severity of bacterial blight.
Intoxicação por Portulaca elatior (Portulacaceae) em bovinos
Authors: Oliveira Neto Temístocles Soares de , Riet-Correa Franklin , Barbosa Francisca Maria Sousa , Nascimento Harlan Hallamys Lima , Carvalho Lucas Rannier Ribeiro Antonino , Alves Alexandre José , Lucena Ricardo Barbosa
Published: November 2017

RESUMO: Descreve-se um surto de intoxicação aguda em bovinos causado pela ingestão de Portulaca elatior na região nordeste do Brasil. O surto resultou da ingestão a partir da administração da planta no cocho pelo criador para os bovinos. Quatro de cinco bovinos foram intoxicados e dois morreram. Os sinais clínicos incluíram dor abdominal, tremores musculares, intensa salivação, timpanismo moderado e desidratação. O curso clínico da doença variou de 10 a 18 horas. Os achados macroscópicos incluíram desidratação, avermelhamento das mucosas dos pré-estômagos, abomaso e intestinos, além de ulceração da mucosa ruminal. Nos dois casos foi notada a presença de fragmentos de Portulaca no interior do rúmen. Os achados histológicos foram de degeneração e necrose das camadas superficiais do epitélio dos pré-estômagos, necrose e inflamação da mucosa intestinal e necrose linfoide. As atividades de AST e GGT estavam dentro dos valores de normalidade. O diagnóstico de intoxicação associado à ingestão de P. elatior foi baseado na epidemiologia, sinais clínicos e lesões. A ausência de informação sobre a toxicidade de P. elatior foi a principal causa para a ocorrência da intoxicação acidental.
Clinical, blood gas and biochemical profile of diarrheic dairy calves fed starter concentrate containing citrus pulp as a replacement for corn
Authors: Soares Marcelo Cezar , Paula Marília Ribeiro de , Slanzon Giovana Simão , Rocha Flávia Hermelina , Mourão Gerson Barreto , Bittar Carla Maris Machado
Published: November 2017

ABSTRACT: The objective of this study was to evaluate clinical signs, gas analysis, and metabolic effects of diarrhea in milk-fed calves consuming starter feed containing citrus pulp (CP) as a replacement for corn. Twenty-four newborn Holstein male calves were distributed into treatments according to starter composition: (1) 0% CP, (2) 32% CP, (3) 64% CP, on dry matter basis. The calves were housed in individual hutches, with free access to water and concentrate, and received 4 L/d of milk replacer. After diarrhea diagnosis, evaluations of fecal score, score of clinical signs and measurement of physiological parameters were performed three times a day during 3-d. Blood samples were collected for electrolytes, blood gases, and plasma biochemical analysis. Starter feed composition had no negative effect (P>0.05) on fecal score, characteristics of diarrheic stools and on the aggravation of diarrhea clinical signs. Biochemical, blood gases and electrolytes changes, as a function of starter composition, did not resulted (P>0.05) in dehydration, acidosis, or other metabolic disturbance animals. Total lactate and D-lactate plasma concentrations were higher for calves on control and 64% CP, and L-lactate was highest for the 64% CP; however, calves showed no signs of metabolic acidosis. Thermal comfort indexes influenced clinical and physiological parameters (P<0.05). Citrus pulp may replace corn in starter composition without prejudice to intestinal health or metabolism of young diarrheic calves.
Doenças parasitárias em bovinos e ovinos no sul do Brasil: frequência e estimativa de perdas econômicas
Authors: Oliveira Plínio Aguiar de , Ruas Jerônimo Lopes , Riet-Correa Franklin , Coelho Ana Carolina B. , Santos Bianca Lemos , Marcolongo-Pereira Clairton , Sallis Eliza Simone Viegas , Schild Ana Lucia
Published: November 2017

RESUMO: Foi realizado um levantamento das doenças parasitárias de bovinos e ovinos diagnosticadas na região sul do Rio Grande do Sul, de janeiro de 1978 a dezembro de 2014 no Laboratório Regional de Diagnóstico da Universidade Federal de Pelotas. Em bovinos 10,1% de todos os diagnosticos tratavam-se de parasitoses, das quais a mais frequente foi a tristeza parasitária bovina, com 55,1% dos surtos. As parasitoses gastrintestinais mistas foram diagnosticadas em 22,35% dos casos, a hemoncose em 4,36%, a dictiocaulose em 3,52%, a fasciolose em 2,68% e a eimeriose em 1,84%. Em ovinos 33,6% dos diagnósticos realizados eram parasitoses, sendo as mais frequentes as parasitoses gastrintestinais mistas (42,7%), a hemoncose (35,4%), a coenurose (9,1%) e a fasciolose (4,4%). Estima-se que as perdas somente por mortalidade, decorrentes de doenças parasitárias em bovinos somam aproximadamente R$16.968.000/ano. Na espécie ovina as perdas econômicas causadas por mortalidade de origem parasitária são de aproximadamente R$2.016.000/ano na região sul do Rio Grande do Sul. Foi possível concluir que, em bovinos, na região sul do Rio Grande do Sul, os agentes da TPB são os principais causadores de perdas econômicas, seguidos pelas parasitoses mistas. Em ovinos a parasitose gastrintestinal mista e a hemoncose, juntas, representam mais de 70% dos diagnósticos realizados.
Detecção de coronavírus em amostras de fezes diarreicas de búfalos ( Bubalus bubalis ) criados no Estado de São Paulo
Authors: Rossi Rodolfo S. , Gaeta Natália C. , Gregori Fabio , Gregory Lilian
Published: November 2017

RESUMO: A diarreia neonatal pode ser consequência de infecções bacterianas, endoparasitarárias e virais. Enquanto esses agentes virais são extensamente estudados nos rebanhos bovinos, faltam informações sobre a importância destes para os rebanhos bubalinos brasileiros. O objetivo deste trabalho foi avaliar a presença de rotavírus e coronavírus em amostras de fezes diarreicas de búfalos (Bubalus buballis) criados em oito propriedades localizadas em Pariquera-açu, Registro, Pirassununga, Dourado, São João da Boa Vista e Congonhas, Estado de São Paulo. Foram coletadas 40 amostras de fezes diarreicas de bezerros búfalos (Bubalus bubalis). A detecção de coronavírus foi realizada pela nested-PCR, enquanto que a detecção de rotavírus foi realizada pela Eletroforese em Gel de Poliacrilamida (PAGE) com coloração com nitrato de prata. Enquanto rotavírus não foi identificado, duas amostras (2/40, 5,0%) foram positivas para coronavírus. Embora no presente trabalho tenha havido baixa detecção de coronavírus e a ausência de rotavírus nos rebanhos estudados, a possível interferência desses vírus no desenvolvimento dos quadros diarreicos não deve ser descartada. Considerando o escasso material encontrado na literatura a respeito da diarreia em bezerros búfalos, principalmente aquele relativo ao coronavírus, nossos resultados são um incentivo para que novos estudos sejam realizados para impulsionar o desenvolvimento da bubalinocultura em nosso país.
Transmissibilidade de Lentivírus de Pequenos Ruminantes para cabritos e cabras adultas por meio de sêmen infectado experimentalmente
Authors: Hasegawa Marjorie Y. , Lara Maria do C.C.S.H. , Gaeta Natália C. , Marques Júlia A. , Ribeiro Bruno L.M. , Rossi Rodolfo S. , Marques Eduardo C. , Gregory Lilian
Published: November 2017

RESUMO: A Artrite Encefalite Caprina se caracteriza por ser multissistêmica e infecciosa, causada por um lentivírus. O estudo teve como objetivo avaliar a transmissibilidade do Lentivírus Caprino, para fêmeas e sua prole, por meio de sêmen infectado experimentalmente. Para tanto, onze fêmeas livres de CAEV foram inseminadas artificialmente com sêmen de bode livre de CAEV ao qual foi adicionado CAEV-Cork para obter título infectante com carga viral em 105 TCID50/ml. (grupo experimental 1). Destas, seis obtiverem prenhez confirmada, e a sua prole (n=6) constituiu o grupo experimental 2. Duas cabras livres de CAEV foram inseminadas artificialmente com sêmen do mesmo bode, sem o inócuo viral, constituindo-se o grupo controle. O diagnóstico da infecção pelo Lentivírus Caprino, foi realizado por IDGA, cELISA e nested-PCR. As fêmeas foram monitoradas durante 210 dias pós inseminação artificial. Já as proles foram imediatamente separadas das mães após o nascimento, e monitoradas nos momentos hora zero, aos quinze dias de idade e mensalmente, até doze meses de idade. Em relação às cabras, 56,96%(9/158) apresentaram positividade para cELISA, 24,05% (38/158) foram positivas a IDGA e nenhuma para nested-PCR. Em relação aos cabritos, 11,28% (15/133) amostras positivas para nested-PCR, 5,26% (7/133) amostras positivas para IDGA e nenhum para cELISA. As proles do grupo controle apresentaram resultados negativos para as três técnicas. A positividade encontrada em nested-PCR pode indicar grande importância para identificação de animais infectados, porém soronegativos, em situações de soroconversão tardia. De acordo com os resultados, concluiu-se que há a transmissão do Lentivírus caprino para a prole e para as mães pelo sêmen infectado.
Pesquisa de anticorpos IgG para Neospora caninum e avaliação dos fatores de risco em ovinos do Estado de Sergipe
Authors: Rizzo Huber , Jesus Taile K.S. de , Gaeta Natália C. , Carvalho Jeferson S. , Pinheiro Júnior José W. , Gregory Lilian , Gennari Solange M. , Villalobos Eliana M.C.
Published: November 2017

RESUMO: O objetivo desse trabalho foi determinar a ocorrência de anticorpos IgG para Neospora caninum bem como avaliar os fatores de risco associados à infecção em rebanhos ovinos do estado de Sergipe, Brasil. Foram coletadas, nos anos de 2011 e 2012, 1200 amostras de sangue de ovinos oriundos de sessenta propriedades distribuídas em três mesorregiões do estado para pesquisa de anticorpos para N. caninum pela Reação de Imunofluorescência Indireta (RIFI) utilizando-se como ponto de corte de 50 e as amostras diluídas na base 2. Os dados de 34 variáveis estudadas foram obtidos a partir de questionários aplicados aos proprietários e analisados para se determinar a frequências absolutas e relativas e análise dos fatores de risco pelo teste Qui-quadrado de Pearson (p≤0,05). A ocorrência de ovinos soropositivos para N. caninum foi de 39,83% (478/1200). Em relação às mesorregiões a ocorrência de animais e propriedades positivas foi de, respectivamente, 55,88% (380/680) e 88,24% (30/34) na Leste; 21,42% (60/280) e 42,85% (6/14) no Agreste e 15,83% (38/240) e 41,67% (5/12) no Sertão. Os títulos de anticorpos variaram de 50, representando 96,02% (459/478) das amostras soropositivas, a 6400 (1/478). Dentre as variáveis significantes, na analise multivariada, que foram consideradas com fatores de risco para a infecção pelo N. caninum estavam propriedades localizadas na mesorregião Leste (p=0,000, OR=4,64, IC95%=3,36-6,41), presença de fonte de água parada e corrente (p=0,000 OR=2,03, IC95%=1,41-2,92), ausência de quarentena (p=0,000 OR=2,71, IC95%=2,08-3,53), não utilização de esterqueiras (p=0,000 OR=3,14, IC95%=2,45-4,02), criações com finalidade de subsistência (p=0,000 OR=4,99, IC95%=3,15-7,92), de reprodução (p=0,002, OR=1,74, IC95%=1,22-2,49), presença de cães (p=0,000 OR=2,74, IC95%=1,73-433) e circulação de animais silvestres nos rebanhos (p=0,000 OR=3,45, IC95%=2,44-4,87). Os resultados evidenciam a ocorrência de N. caninum em rebanhos ovinos sergipanos, demonstrando o manejo e a localização dos rebanhos no estado como importantes fatores de risco.
Suppurative infectious diseases of the central nervous system in domestic ruminants
Authors: Konradt Guilherme , Bassuino Daniele M. , Prates Klaus S. , Bianchi Matheus V. , Snel Gustavo G.M. , Sonne Luciana , Driemeier David , Pavarini Saulo P.
Published: November 2017

ABSTRACT: This study describes suppurative infectious diseases of the central nervous system (CNS) in domestic ruminants of southern Brazil. Reports from 3.274 cattle, 596 sheep and 391 goats were reviewed, of which 219 cattle, 21 sheep and 7 goats were diagnosed with central nervous system inflammatory diseases. Suppurative infectious diseases of the CNS corresponded to 54 cases (28 cattle, 19 sheep and 7 goats). The conditions observed consisted of listerial meningoencephalitis (8 sheep, 5 goats and 4 cattle), suppurative leptomeningitis and meningoencephalitis (14 cattle, 2 goats and 1 sheep), cerebral (6 cattle and 2 sheep), and spinal cord (7 sheep) abscesses, and basilar empyema (4 cattle and 1 sheep). Bacterial culture identified Listeria monocytogenes (9/54 cases), Escherichia coli (7/54 cases), Trueperella pyogenes (6/54 cases) and Proteus mirabilis (1/54 cases). All cases diagnosed as listeriosis through histopathology yielded positive immunostaining on immunohistochemistry, while 12/17 of the cases of suppurative leptomeningitis and meningoencephalitis presented positive immunostaining for Escherichia coli. Meningoencephalitis by L. monocytogenes was the main neurological disease in sheep and goats, followed by spinal cord abscesses in sheep. In cattle, leptomeningitis and suppurative meningoencephalitis was the most frequent neurological disease for the species, and E. coli was the main cause of these lesions. Basilar empyema, mainly diagnosed in cattle, is related to traumatic injuries, mainly in the nasal cavity, and the main etiologic agent was T. pyogenes.
Intoxicação natural e experimental por Leucaena leucocephala em equinos
Authors: Porto Mirna R. , Moscardini Augusto R.C. , Novais Ernane P.F. , Cabral Filho Sérgio L.S. , Lima Eduardo M.M. , Castro Márcio B.
Published: November 2017

RESUMO: Este trabalho investigou as principais alterações clínicas e dermatopatológicas de equinos intoxicados natural e experimentalmente por Leucaena leucocephala. Os surtos ocorreram nos estados de São Paulo e Goiás, onde seis cavalos após a ingestão de casca e/ou folhas de L. leucocephala apresentaram alopecia, principalmente na crina e cauda. Nesses animais o diagnóstico foi baseado na observação da ingestão da planta e dos sinais clínicos. Nos animais intoxicados experimentalmente, foi realizado o exame clínico, biópsias da pele das regiões de crina, dorso e cauda e foi feita dosagem sérica de tri-iodotironina (T3) e tiroxina (T4). Alopecia da crina e cauda foi o principal sinal clínico observado, seguido de anorexia, emagrecimento e apatia em todos os equinos. Os níveis de T3 e T4 total apresentaram redução significativa (p≤0,05) na terceira semana de ingestão da leucena quando comparado aos níveis basais. As alterações histológicas observadas nas biópsias de pele demonstram acentuada telogenização dos folículos pilosos ao final dos experimentos. Os achados clínico-patológicos em equinos intoxicados são semelhantes aos observados em ruminantes. A intoxicação experimental de equinos por L. leucocephala evidenciou o acentuado declínio dos níveis dos hormônios tireoideanos, sugerindo efeito bociogênico da mimosina e seus compostos derivados, com hipotireoidismo transitório e alopecia devido à acentuada redução da atividade folicular.
Avaliação clínica de cães com doença do disco intervertebral (Hansen tipo I) submetidos à descompressão cirúrgica: 110 casos
Authors: Chaves Rafael O. , Polidoro Dakir N. , Feranti João P.S. , Fabretti Andrei K. , Copat Bruna , Gomes Lucas A. , Mazzanti Alexandre
Published: November 2017

RESUMO: O objetivo deste estudo foi identificar cães com doença do disco intervertebral toracolombar (Hansen tipo I) submetidos ao tratamento cirúrgico em um serviço de rotina em neurologia de um hospital veterinário universitário no período de 2006 a 2014, e obter informações a respeito da idade, do sexo, da raça, local da extrusão, interpretação da mielografia, avaliação dos graus de disfunção neurológica, recuperação funcional desses cães e observar a ocorrência de recidiva dos sinais clínicos da doença. A raça mais frequente foi Dachshund (69%), seguida dos cães sem raça definida (14,5%). Os locais mais afetados foram entre T12-T13 (31,9%) e L1-L2 (19,1%). Dos 110 cães, 74 (67,3%) tiveram melhora dos sinais clínicos após o procedimento cirúrgico, sendo 54 (49,1%) considerados satisfatórios e 20 (18,2%), parcialmente satisfatórios. Destes cães, seis (8,1%) cães estavam em grau II, 19 (25,7%) em grau III, 35 (47,3%) em grau IV e 14 (18,9%) em grau V. Pode-se concluir que o tratamento cirúrgico promove recuperação funcional satisfatória na maioria dos cães com extrusão de disco toracolombar. O prognóstico para recuperação funcional após o tratamento cirúrgico é tanto melhor quanto menor for o grau de disfunção neurológica e o percentual de recidiva é baixo em animais submetidos a este tipo de terapia.
Acute and chronic toxicity and antimicrobial activity of the extract of Stryphnodendron adstringens (Mart.) Coville
Authors: Almeida Anna C. , Andrade Viviane A. , Fonseca Francine S.A. , Macêdo Auricélio A. , Santos Renato L. , Colen Keila G.F. , Martins Ernane R. , Marcelo Natália A.
Published: November 2017

ABSTRACT: This study evaluated the antimicrobial activity and acute or chronic toxicity of the extract of Stryphnodendron adstringens. The stem bark dry extract was obtained by static maceration with ethanol. Quantification of tannins was performed by the Folin-Denis method, which indicated a total tannin content of 32.7%. The antimicrobial activity of the dry extract of S. adstringens was evaluated by agar-based disk diffusion assay with Escherichia coli (ATCC 25922) and Staphylococcus aureus (ATCC 25923) in the concentration of 200, 400 and 600μL/mL. The results indicated that 600μL/mL inhibited microbial growth, i.e. had antimicrobial activity against these species. Acute and chronic toxic effects of S. adstringens was evaluated in Wistar rats treated with 200, 400, 600 and 800mg/kg of extract, administrated by gavage. Liver degeneration was observed in the group of rats receiving 800mg/kg in chronic exposure, what may indicate some degree of toxicity at this concentration. However, no systemic toxicity was observed at lower doses. Considering the broad use of S. adstringens as a phytotherapeutic agent for various human and animal diseases and the livertoxicity observed at high concentrations, attention should be paid to the possible adverse effect of using the extract from this plant at high concentration.
Increased nitric oxide plasma concentration in dogs with naturally acquired chronic renal disease
Authors: Galvão André B. , Carvalho Marileda B. , Batalhão Luciane G. , Silva Juliana C.B. , Batalhão Marcelo , Carnio Evelin C.
Published: November 2017

ABSTRACT: This study aimed to determine the amount of plasma nitric oxide in clinically stable dogs at different stages of chronic kidney disease (CKD). Five groups of dogs were studied, aged from 4 to 18, comprising of a control group composed of healthy animals (control n=17), group CKD stage 1 (DRC-1, n=12), group CKD stage 2 (CKD-2, n=10) group, CKD stages 3 (CRD-3, n=13) and Group CKD stage 4 (DRC-4, n=10). Dogs with CKD were clinically stable and received no treatment. Two blood samples were collected at 24 hours intervals (repeated measures) to obtain serum and plasma. The serum creatinine values were used to classify dogs as CG, CKD-1, CKD-2, CKD-3 and CKD-4, and were (1.02±0.02mg/dL), (1.07±0.04mg/dL), (1.81±0.03mg/dL), (3.40±0.15mg/dL) and (6.00±0.20mg/dL) respectively. The determination of nitric oxide (NO) was performed by dosing nitrate/nitrite indirectly, and used for measurement of nitrate according to the NO/ozone chemiluminescence. The data were submitted to ANOVA for nonparametric analysis(Kruskal-Wallis) (P<0.05). The concentration of plasmatic NO did not differ significantly among GC (10.81±0.51μM), CKD-1 (15.49±1.97μM) and CKD-2 (19.83±3.31μM) groups. The plasma concentration of CKD-3 (17.02±1.73μM) and CKD-4 (83.56±13.63μM) was significantly higher compared with healthy dogs. In conclusion, the NO plasma concentration can increase in dogs with CKD and become significantly higher in stage 3 and 4 dogs.
Desenvolvimento de caminhar espinal em cães paraplégicos com fraturas e luxações vertebrais toracolombares
Authors: Araújo Bruno Martins , Fernandes Thaíza Helena Tavares , Baraúna Junior Durval , Bonelli Marília de Albuquerque , Amorim Marcela Maria de Almeida , Tudury Eduardo Alberto
Published: November 2017

RESUMO: Fraturas e luxações vertebrais (FLV) toracolombares estão dentre as afecções neurológicas mais frequentes na neurologia veterinária. São um dos distúrbios mais graves e desafiadores, devido ao elevado risco de paralisia permanente, levando muitos animais a serem submetidos à eutanásia, devido ao prognóstico desfavorável nos animais que perderam a nocicepção. Objetivou-se descrever as bases neurofisiológicas responsáveis pelo desenvolvimento do caminhar espinal e analisar, em 37 cães acometidos por FLV toracolombares, os dados referentes à taxa de recuperação dos animais com e sem nocicepção. Naqueles sem nocicepção, analisou-se ainda a frequência dos animais que desenvolveram caminhar espinal e o período médio para seu aparecimento. Em relação ao grau da lesão a as taxas de recuperação, 14/37 animais (37,8%) possuíam nocicepção, no qual a taxa de recuperação da deambulação voluntaria e das funções viscerais foi de 100%. Enquanto que 23/37 animais (62,1%) perderam a nocicepção, no qual nenhum recuperou a deambulação voluntária, ocorrendo morte por causas diversas em sete destes. Dos 16 animais sem nocicepção sobreviventes e que foram submetidos ao tratamento conservativo ou cirúrgico, cinco (31,25%) readquiriram a capacidade de caminhar (tempo médio de 115 dias) sem recuperar a nocicepção, sendo esta deambulação involuntária atribuída ao caminhar espinal. De acordo com os resultados desta pesquisa, o parâmetro isolado da perda da nocicepção não deve desencorajar a realização da terapia, pois em cães paraplégicos com FLV toracolombares, há possibilidade de ocorrer desenvolvimento de deambulação involuntária.