bluuebunny/crossref_metadata_2025 huggingface dataset:
The Dipeptidyl Peptidase-4 Inhibitor Linagliptin Directly Enhances the Contractile Recovery of Mouse Hearts at a Concentration Equivalent to that Achieved with Standard Dosing in Humans
Authors: Batchu Sri Nagarjun , Yerra Veera Ganesh , Liu Youan , Advani Suzanne L. , Klein Thomas , Advani Andrew
Published: August 2020

Despite a similar mechanism of action underlying their glucose-lowering effects in type 2 diabetes, dipeptidyl peptidase-4 (DPP-4) inhibitors have diverse molecular structures, raising the prospect of agent-specific, glucose-independent actions. To explore the issue of possible DPP-4 inhibitor cardiac heterogeneity, we perfused different DPP-4 inhibitors to beating mouse hearts ex vivo, at concentrations equivalent to peak plasma levels achieved in humans with standard dosing. We studied male and female mice, young non-diabetic mice, and aged diabetic high fat diet-fed mice and observed that linagliptin enhanced recovery after ischemia-reperfusion, whereas sitagliptin, alogliptin, and saxagliptin did not. DPP-4 transcripts were not detected in adult mouse cardiomyocytes by RNA sequencing and the addition of linagliptin caused ≤0.2% of cardiomyocyte genes to be differentially expressed. In contrast, incubation of C166 endothelial cells with linagliptin induced cell signaling characterized by phosphorylation of Akt and endothelial nitric oxide synthase, whereas the nitric oxide (NO) donor, S-nitroso-N-acetylpenicillamine increased serine 16 phosphorylation of the calcium regulatory protein, phospholamban in cardiomyocytes. Furthermore, linagliptin increased cardiomyocyte cGMP when cells were co-cultured with C166 endothelial cells, but not when cardiomyocytes were cultured alone. Thus, at a concentration comparable to that achieved in patients, linagliptin has direct effects on mouse hearts. The effects of linagliptin on cardiomyocytes are likely to be either off-target or indirect, mediated through NO generation by the adjacent cardiac endothelium.
Medaka (Oryzias latipes) Embryo as a Model for the Screening of Compounds That Counteract the Damage Induced by Ultraviolet and High-Energy Visible Light
Authors: Merino Marián , Mullor José , Sánchez-Sánchez Ana
Published: August 2020

Continuous overexposure to sunlight increases its harmful effects on the skin. For this reason, there is a growing need to characterize economic models more representative of the negative effects and counteracting responses that irradiation causes on human skin. These models will serve for the screening of protective compounds against damage caused by ultraviolet (UV) and high energy visible light (HEV). Therefore, two common in vitro models employed for sunlight irradiation studies, namely human keratinocyte HaCat culture and reconstructed human epidermis (RHE), were compared with the medaka fish embryo model, traditionally used in other scientific disciplines. Using suberythemal doses of UVA and HEV to determine the level of Reactive Oxygen Species (ROS) generation and thymine dimers formed by UVB, we show that medaka embryo responds with a lower damage level, more comparable to human skin, than the other two models, probably due to the protective mechanisms that work in a complete organism. In the same way, the protective effects of antioxidant compounds have the greatest effect on medaka embryos. Taken together, these findings suggest that medaka embryos would be a good alternative in vitro model for sunlight effect studies, and for the screening of molecules with counteracting capacity against the damage caused by UV and HEV.
The Effects of Physical Exercise on Balance and Prevention of Falls in Older People: A Systematic Review and Meta-Analysis
Authors: Papalia Giuseppe Francesco , Papalia Rocco , Diaz Balzani Lorenzo Alirio , Torre Guglielmo , Zampogna Biagio , Vasta Sebastiano , Fossati Chiara , Alifano Anna Maria , Denaro Vincenzo
Published: August 2020

The aims of this systematic review and meta-analysis were to evaluate the effects of physical exercise on static and dynamic balance in the elderly population, and to analyze the number of falls and fallers. A systematic literature search was conducted using PubMed–Medline, Cochrane Central, and Google Scholar to select randomized clinical trials that analyzed the role of exercise on balance and fall rate in patients aged 65 or older. Sixteen articles were included in this review. Applying the Cochrane risk-of-bias tool, three studies were determined to be at low risk of bias, nine at unclear risk of bias, and four at high risk of bias. The meta-analysis showed improvements in dynamic balance (p = 0.008), static balance (p = 0.01), participants’ fear of falling (p = 0.10), balance confidence (p = 0.04), quality of life (p = 0.08), and physical performance (p = 0.30) in patients who underwent physical exercise compared to controls. The analysis of the total numbers of falls showed a decreased likelihood of falls in patients who participated in exercise programs (p = 0.0008). Finally, the number of patients who fell at least once was significantly reduced in the intervention group (p = 0.02). Physical exercise is an effective treatment to improve balance and reduce fall rates in the elderly.
Mechanisms of Mitochondrial Dysfunction in Lysosomal Storage Disorders: A Review
Authors: Stepien Karolina M. , Roncaroli Federico , Turton Nadia , Hendriksz Christian J. , Roberts Mark , Heaton Robert A. , Hargreaves Iain
Published: August 2020

Mitochondrial dysfunction is emerging as an important contributory factor to the pathophysiology of lysosomal storage disorders (LSDs). The cause of mitochondrial dysfunction in LSDs appears to be multifactorial, although impaired mitophagy and oxidative stress appear to be common inhibitory mechanisms shared amongst these heterogeneous disorders. Once impaired, dysfunctional mitochondria may impact upon the function of the lysosome by the generation of reactive oxygen species as well as depriving the lysosome of ATP which is required by the V-ATPase proton pump to maintain the acidity of the lumen. Given the reported evidence of mitochondrial dysfunction in LSDs together with the important symbiotic relationship between these two organelles, therapeutic strategies targeting both lysosome and mitochondrial dysfunction may be an important consideration in the treatment of LSDs. In this review we examine the putative mechanisms that may be responsible for mitochondrial dysfunction in reported LSDs which will be supplemented with morphological and clinical information.
Should We Report 15q11.2 BP1-BP2 Deletions and Duplications in the Prenatal Setting?
Authors: Maya Idit , Perlman Sharon , Shohat Mordechai , Kahana Sarit , Yacobson Shiri , Tenne Tamar , Agmon-Fishman Ifaat , Tomashov Matar Reut , Basel-Salmon Lina , Sukenik-Halevy Rivka
Published: August 2020

Copy number variations of the 15q11.2 region at breakpoints 1-2 (BP1-BP2) have been associated with variable phenotypes and low penetrance. Detection of such variations in the prenatal setting can result in significant parental anxiety. The clinical significance of pre- and postnatally detected 15q11.2 BP1-BP2 deletions and duplications was assessed. Of 11,004 chromosomal microarray tests performed in a single referral lab (7596 prenatal, 3408 postnatal), deletions were detected in 66 cases: 39 in prenatal tests (0.51%) and 27 in postnatal tests (0.79%). Duplications were detected in 94 cases: 62 prenatal tests (0.82%) and 32 postnatal tests (0.94%). The prevalence of deletions and duplications among clinically indicated prenatal tests (0.57% and 0.9%, respectively) did not differ significantly in comparison to unindicated tests (0.49% and 0.78%, respectively). The prevalence of deletions and duplications among postnatal tests performed for clinical indications was similar to the prevalence in healthy individuals (0.73% and 1% vs. 0.98% and 0.74%, respectively). The calculated penetrance of deletions and duplications over the background risk was 2.18% and 1.16%, respectively. We conclude that the pathogenicity of 15q11.2 BP1-BP2 deletions and duplications is low. Opting out the report of these copy number variations to both clinicians and couples should be considered.
Prediction of Adverse Events in Stable Non-Variceal Gastrointestinal Bleeding Using Machine Learning
Authors: Seo Dong-Woo , Yi Hahn , Park Beomhee , Kim Youn-Jung , Jung Dae Ho , Woo Ilsang , Sohn Chang Hwan , Ko Byuk Sung , Kim Namkug , Kim Won Young
Published: August 2020

Clinical risk-scoring systems are important for identifying patients with upper gastrointestinal bleeding (UGIB) who are at a high risk of hemodynamic instability. We developed an algorithm that predicts adverse events in patients with initially stable non-variceal UGIB using machine learning (ML). Using prospective observational registry, 1439 out of 3363 consecutive patients were enrolled. Primary outcomes included adverse events such as mortality, hypotension, and rebleeding within 7 days. Four machine learning algorithms, namely, logistic regression with regularization (LR), random forest classifier (RF), gradient boosting classifier (GB), and voting classifier (VC), were compared with the Glasgow–Blatchford score (GBS) and Rockall scores. The RF model showed the highest accuracies and significant improvement over conventional methods for predicting mortality (area under the curve: RF 0.917 vs. GBS 0.710), but the performance of the VC model was best in hypotension (VC 0.757 vs. GBS 0.668) and rebleeding within 7 days (VC 0.733 vs. GBS 0.694). Clinically significant variables including blood urea nitrogen, albumin, hemoglobin, platelet, prothrombin time, age, and lactate were identified by the global feature importance analysis. These results suggest that ML models will be useful early predictive tools for identifying high-risk patients with initially stable non-variceal UGIB admitted at an emergency department.
Low PCSK-9 levels Are Associated with Favorable Neurologic Function after Resuscitation from out of Hospital Cardiac Arrest
Authors: Merrelaar Anne , Buchtele Nina , Schriefl Christoph , Clodi Christian , Poppe Michael , Ettl Florian , Herkner Harald , Jilma Bernd , Schwameis Michael , Schoergenhofer Christian
Published: August 2020

Endotoxemia after cardiopulmonary resuscitation (CPR) is associated with unfavorable outcome. Proprotein convertase subtilisin/kexin type-9 (PCSK–9) regulates low-density lipoprotein receptors, which mediate the hepatic uptake of endotoxins. We hypothesized that PCSK–9 concentrations are associated with neurological outcome in patients after CPR. Successfully resuscitated out-of-hospital cardiac arrest patients were included prospectively (n = 79). PCSK–9 levels were measured on admission, 12 h and 24 h thereafter, and after rewarming. The primary outcome was favorable neurologic function at day 30, defined by cerebral performance categories (CPC 1–2 = favorable vs. CPC 3–5 = unfavorable). Receiver operating characteristic curve analysis was used to identify the PCSK–9 level cut-off for optimal discrimination between favorable and unfavorable 30-day neurologic function. Logistic regression models were calculated to estimate the effect of PCSK–9 levels on the primary outcome, given as odds ratio (OR) and 95% confidence interval (95%CI). PCSK–9 levels on admission were significantly lower in patients with favorable 30-day neurologic function (median 158 ng/mL, (quartiles: 124–225) vs. 207 ng/mL (174–259); p = 0.019). The optimally discriminating PCSK–9 level cut-off was 165 ng/mL. In patients with PCSK–9 levels ≥ 165 ng/mL, the odds of unfavorable neurological outcome were 4.7-fold higher compared to those with PCSK–9 levels < 165 ng/mL. In conclusion, low PCSK–9 levels were associated with favorable neurologic function.
Biologic Therapy in Refractory Non-Multiple Sclerosis Optic Neuritis Isolated or Associated to Immune-Mediated Inflammatory Diseases. A Multicenter Study
Authors: Herrero-Morant Alba , Álvarez-Reguera Carmen , Martín-Varillas José L. , Calvo-Río Vanesa , Casado Alfonso , Prieto-Peña Diana , Atienza-Mateo Belén , Maiz-Alonso Olga , Blanco Ana , Vicente Esther , Rúa-Figueroa Íñigo , Cáceres-Martin Laura , García-Serrano José L. , Callejas-Rubio José Luis , Ortego-Centeno Norberto , Narváez Javier , Romero-Yuste Susana , Sánchez Julio , Estrada Paula , Demetrio-Pablo Rosalía , Martínez-López David , Castañeda Santos , Hernández José L. , González-Gay Miguel Á. , Blanco Ricardo
Published: August 2020

We aimed to assess the efficacy of biologic therapy in refractory non-Multiple Sclerosis (MS) Optic Neuritis (ON), a condition more infrequent, chronic and severe than MS ON. This was an open-label multicenter study of patients with non-MS ON refractory to systemic corticosteroids and at least one conventional immunosuppressive drug. The main outcomes were Best Corrected Visual Acuity (BCVA) and both Macular Thickness (MT) and Retinal Nerve Fiber Layer (RNFL) using Optical Coherence Tomography (OCT). These outcome variables were assessed at baseline, 1 week, and 1, 3, 6 and 12 months after biologic therapy initiation. Remission was defined as the absence of ON symptoms and signs that lasted longer than 24 h, with or without an associated new lesion on magnetic resonance imaging with gadolinium contrast agents for at least 3 months. We studied 19 patients (11 women/8 men; mean age, 34.8 ± 13.9 years). The underlying diseases were Bechet’s disease (n = 5), neuromyelitis optica (n = 3), systemic lupus erythematosus (n = 2), sarcoidosis (n = 1), relapsing polychondritis (n = 1) and anti-neutrophil cytoplasmic antibody -associated vasculitis (n = 1). It was idiopathic in 6 patients. The first biologic agent used in each patient was: adalimumab (n = 6), rituximab (n = 6), infliximab (n = 5) and tocilizumab (n = 2). A second immunosuppressive drug was simultaneously used in 11 patients: methotrexate (n = 11), azathioprine (n = 2), mycophenolate mofetil (n = 1) and hydroxychloroquine (n = 1). Improvement of the main outcomes was observed after 1 year of therapy when compared with baseline data: mean ± SD BCVA (0.8 ± 0.3 LogMAR vs. 0.6 ± 0.3 LogMAR; p = 0.03), mean ± SD RNFL (190.5 ± 175.4 μm vs. 183.4 ± 139.5 μm; p = 0.02), mean ± SD MT (270.7 ± 23.2 μm vs. 369.6 ± 137.4 μm; p = 0.03). Besides, the median (IQR) prednisone-dose was also reduced from 40 (10–61.5) mg/day at baseline to. 2.5 (0–5) mg/day after one year of follow-up; p = 0.001. After a mean ± SD follow-up of 35 months, 15 patients (78.9%) achieved ocular remission, and 2 (10.5%) experienced severe adverse events. Biologic therapy is effective in patients with refractory non-MS ON.
Numerical and Experimental Investigation of the Through-Thickness Strength Properties of Woven Glass Fiber Reinforced Polymer Composite Laminates under Combined Tensile and Shear Loading
Authors: Kanno Teruyoshi , Kurita Hiroki , Suzuki Masashi , Tamura Hitoshi , Narita Fumio
Published: August 2020

The purpose of this paper is to investigate the through-thickness stresses of woven glass fiber reinforced polymer (GFRP) composite laminates under combined tensile and shear loading. Tensile tests were carried out with cross specimens at room temperature under various stacking angles, and the through-thickness strength properties of the woven GFRP laminates were evaluated. The failure characteristics of the woven GFRP laminates were also studied by optical microscopy observations. A three-dimensional finite element analysis (FEA) was carried out to calculate the stress distributions in the cross specimens, and the failure conditions of the specimens were examined. The numerically determined interlaminar tensile and shear stresses at failure location were consistent with Hoffman and Mohr-Coulomb failure criteria when the stacking angle was relatively small. This work is the first attempt to quantify the relation between interlaminar tensile and shear strengths of GFRP composite laminates under tensile and shear loading simultaneously using a combined numerical and experimental approach. A method based on finite element stress analysis was developed for estimating the through-thickness strength of the composite laminates using the experimentally determined fracture load and location. The results suggest that the through-thickness strength under combined tensile and shear loading can be determined effectively by this approach for relatively small stacking angles.
Full 3D Microwave Breast Imaging Using a Deep-Learning Technique
Authors: Khoshdel Vahab , Asefi Mohammad , Ashraf Ahmed , LoVetri Joe
Published: August 2020

A deep learning technique to enhance 3D images of the complex-valued permittivity of the breast obtained via microwave imaging is investigated. The developed technique is an extension of one created to enhance 2D images. We employ a 3D Convolutional Neural Network, based on the U-Net architecture, that takes in 3D images obtained using the Contrast-Source Inversion (CSI) method and attempts to produce the true 3D image of the permittivity. The training set consists of 3D CSI images, along with the true numerical phantom images from which the microwave scattered field utilized to create the CSI reconstructions was synthetically generated. Each numerical phantom varies with respect to the size, number, and location of tumors within the fibroglandular region. The reconstructed permittivity images produced by the proposed 3D U-Net show that the network is not only able to remove the artifacts that are typical of CSI reconstructions, but it also enhances the detectability of the tumors. We test the trained U-Net with 3D images obtained from experimentally collected microwave data as well as with images obtained synthetically. Significantly, the results illustrate that although the network was trained using only images obtained from synthetic data, it performed well with images obtained from both synthetic and experimental data. Quantitative evaluations are reported using Receiver Operating Characteristics (ROC) curves for the tumor detectability and RMS error for the enhancement of the reconstructions.
Effect of the Geometric Profile of Top Ring on the Tribological Characteristics of a Low-Displacement Diesel Engine
Authors: Duarte Forero Jorge , Valencia Ochoa Guillermo , Piero Rojas Jhan
Published: August 2020

The present study aims to analyze the influence of the geometric profile of the compression ring on the tribological properties of the lubricant. Additionally, the influence of the rotation speed and the engine load on the state of the lubricant is evaluated. For this study, a single-cylinder diesel engine is taken as the basis, from which a CAD model of the combustion chamber-piston assembly was made. In addition, the conditions in the cylinder chamber were analyzed when the engine operates at a rotation speed of 3000, 3300, 3600, and 3900 rpm, and a load of 1.5, 3.0, 4.5, and 6.0 N. The calculations were developed using the OpenFOAM® simulation software. The results obtained show that changes in the geometric profile of the ring can contribute to reducing the hydrodynamic friction force by 13% and the friction force caused by roughness by 61%. This implies a decrease in the power lost by friction. In general, the modification of the geometric profile allowed a reduction of 21% in the lost power associated with friction. Additionally, it was observed that the shape of the profile allows to reduce the pressure in the lubricant by 65% and obtain a greater thickness of the lubrication film. On average, an increase of 300 rpm and 1.5 N in the speed and load of the engine causes the friction force and power losses to increase by 45% and 10%. The above results imply that the geometric profile of the compression ring can improve tribological performance in the engine, allowing a reduction in fuel and better lubricant performance.
Tensile Strength and Degradation of GFRP Bars under Combined Effects of Mechanical Load and Alkaline Solution
Authors: Jin Qingping , Chen Peixia , Gao Yonghong , Du Aihua , Liu Dongxu , Sun Lizhi
Published: August 2020

Mechanical properties of glass fiber reinforced polymer (GFRP) composites degrade under the combined effects of mechanical load and alkaline solution, affecting the service ability and safety of GFRP reinforced structures. In this study, GFRP bars were loaded with cyclic tension at different stress levels and immersed in alkaline solution for days to investigate the tensile properties and degradation law of GFRP bars. The degradation mechanisms were studied at micro-, meso- and macro-scales with scanning electron microscopy (SEM) and three-dimensional X-ray microscopy, respectively. The results show that tensile strength and degradation rate of GFRP bars are mainly dependent on the different stress levels and alkaline solution. When stress level is higher, the tensile strength degrades more quickly, especially in the early stages of soaking. With the loading and immersion time, the elastic modulus and Poisson’s ratio increase at first and then decrease. The ultimate tensile strain is relatively stable, whereas the ultimate elongation is significantly reduced. A strength-degradation model was proposed and fit well with experimental data, demonstrating that the model can be applied to predict tensile strength degradation under combined effects of the load and alkaline solution.
Effect of Electronic Correlations on the Electronic Structure, Magnetic and Optical Properties of the Ternary RCuGe Compounds with R = Tb, Dy, Ho, Er
Authors: Lukoyanov Alexey V. , Gramateeva Lubov N. , Knyazev Yury V. , Kuz’min Yury I. , Gupta Sachin , Suresh K. G.
Published: August 2020

In this study, the ab initio and experimental results for RCuGe ternary intermetallics were reported for R = Tb, Dy, Ho, Er. Our theoretical calculations of the electronic structure, employing local spin density approximation accounting for electron–electron correlations in the 4f shell of Tb, Dy, Ho, Er ions were carried in DFT+U method. The optical properties of the RCuGe ternary compounds were studied at a broad range of wavelengths. The spectral and electronic characteristics were obtained. The theoretical electron densities of states were taken to interpret the experimental energy dependencies of the experimental optical conductivity in the interband light–absorption region. From the band calculations, the 4f shell of the rare-earth ions was shown to provide the major contribution to the electronic structure, magnetic and optical properties of the RCuGe intermetallics. The accounting for electron–electron correlations in Tb, Dy, Ho, Er resulted in a good agreement between the calculated and experimental magnetic and optical characteristics.
Chemiresistive Properties of Imprinted Fluorinated Graphene Films
Authors: Sysoev Vitalii I. , Bulavskiy Mikhail O. , Pinakov Dmitry V. , Chekhova Galina N. , Asanov Igor P. , Gevko Pavel N. , Bulusheva Lyubov G. , Okotrub Alexander V.
Published: August 2020

The electrical conductivity of graphene materials is strongly sensitive to the surface adsorbates, which makes them an excellent platform for the development of gas sensor devices. Functionalization of the surface of graphene opens up the possibility of adjusting the sensor to a target molecule. Here, we investigated the sensor properties of fluorinated graphene films towards exposure to low concentrations of nitrogen dioxide NO2. The films were produced by liquid-phase exfoliation of fluorinated graphite samples with a composition of CF0.08, CF0.23, and CF0.33. Fluorination of graphite using a BrF3/Br2 mixture at room temperature resulted in the covalent attachment of fluorine to basal carbon atoms, which was confirmed by X-ray photoelectron and Raman spectroscopies. Depending on the fluorination degree, the graphite powders had a different dispersion ability in toluene, which affected an average lateral size and thickness of the flakes. The films obtained from fluorinated graphite CF0.33 showed the highest relative response ca. 43% towards 100 ppm NO2 and the best recovery ca. 37% at room temperature.
Daylight-Active Cellulose Nanocrystals Containing Anthraquinone Structures
Authors: Zhu Yiwen , Sulkanen Audrey , Liu Gang-Yu , Sun Gang
Published: August 2020

Antimicrobial and antiviral materials have attracted significant interest in recent years due to increasing occurrences of nosocomial infections and pathogenic microbial contamination. One method to address this is the combination of photoactive compounds that can produce reactive oxygen species (ROS), such as hydrogen peroxide and hydroxyl radicals to disinfect microbes, with carrier materials that meet the application requirements. Using anthraquinone (AQ) and cellulose nanocrystals (CNCs) as the photoactive and carrier components, respectively, this work demonstrated the first covalent incorporation of AQ onto CNCs. The morphology and the photoactive properties were investigated, revealing the structural integrity of the CNCs and the high degree of photoactivity of the AQ-CNC materials upon UVA exposure. The AQ-CNCs also exhibited an unexpected persistent generation of ROS under darkness, which adds advantages for antimicrobial applications.
Coefficient Estimates for a Subclass of Analytic Functions Associated with a Certain Leaf-Like Domain
Authors: Khan Bilal , Srivastava Hari M. , Khan Nazar , Darus Maslina , Tahir Muhammad , Ahmad Qazi Zahoor
Published: August 2020

First, by making use of the concept of basic (or q-) calculus, as well as the principle of subordination between analytic functions, generalization Rq(h) of the class R(h) of analytic functions, which are associated with the leaf-like domain in the open unit disk U, is given. Then, the coefficient estimates, the Fekete–Szegö problem, and the second-order Hankel determinant H2(1) for functions belonging to this class Rq(h) are investigated. Furthermore, similar results are examined and presented for the functions zf(z) and f−1(z). For the validity of our results, relevant connections with those in earlier works are also pointed out.
Hypercompositional Algebra, Computer Science and Geometry
Authors: Massouros Gerasimos , Massouros Christos
Published: August 2020

The various branches of Mathematics are not separated between themselves. On the contrary, they interact and extend into each other’s sometimes seemingly different and unrelated areas and help them advance. In this sense, the Hypercompositional Algebra’s path has crossed, among others, with the paths of the theory of Formal Languages, Automata and Geometry. This paper presents the course of development from the hypergroup, as it was initially defined in 1934 by F. Marty to the hypergroups which are endowed with more axioms and allow the proof of Theorems and Propositions that generalize Kleen’s Theorem, determine the order and the grade of the states of an automaton, minimize it and describe its operation. The same hypergroups lie underneath Geometry and they produce results which give as Corollaries well known named Theorems in Geometry, like Helly’s Theorem, Kakutani’s Lemma, Stone’s Theorem, Radon’s Theorem, Caratheodory’s Theorem and Steinitz’s Theorem. This paper also highlights the close relationship between the hyperfields and the hypermodules to geometries, like projective geometries and spherical geometries.
Analysis of the Oscillation Behavior of Hybrid Aluminum/Steel Joints Realized by Ultrasound Enhanced Friction Stir Welding
Authors: Thomä Marco , Gester Andreas , Wagner Guntram , Fritzsche Marco
Published: August 2020

Friction stir welding (FSW) is an innovative solid-state joining process, which is suitable for joining dissimilar materials with strongly differing physical and chemical properties such as aluminum and steel. Where other joining methods such as fusion welding struggle to achieve appropriate joint strengths due to the excessive formation of brittle aluminum-rich intermetallic phases (IMP), FSW joints of aluminum and steel only show small layers of IMP, thus, sufficient tensile strengths in proximity to the maximum tensile strength of the weaker aluminum base material can be reached. With the aim to optimize the mechanical and microstructural properties of such dissimilar joints for widening the field for possible industrial applications, several hybrid friction stir welding methods have been developed which include an additional energy input, whereas the ultrasound enhancement (USE-FSW) is one of the most promising. The current work was carried out on AA6061/DC04 joints which were successfully friction stir welded with and without ultrasound support, in respect to the influence of varying the ultrasound transmission side. The functionality of the USE-FSW setup could be verified by multi point laser vibrometer measurements. Additionally, a higher proportion of transversal oscillation for the transmission of power ultrasound into aluminum could be detected using a scanning vibrometer. In comparison to the conventionally friction stir welded joints the ultrasound enhancement led to an avoidance of weld defects and an increase of the steel particle volume in the stir zone. The joint produced with power ultrasound transmission via aluminum resulted in a more uniform interface.
Spark Plasma Sintering of Titanium Aluminides: A Progress Review on Processing, Structure-Property Relations, Alloy Development and Challenges
Authors: Mogale Ntebogeng F. , Matizamhuka Wallace R.
Published: August 2020

Titanium aluminides (TiAl) have the potential of substituting nickel-based superalloys (NBSAs) in the aerospace industries owing to their lightweight, good mechanical and oxidation properties. Functional simplicity, control of sintering parameters, exceptional sintering speeds, high reproducibility, consistency and safety are the main benefits of spark plasma sintering (SPS) over conventional methods. Though TiAl exhibit excellent high temperature properties, SPS has been employed to improve on the poor ductility at room temperature. Powder metallurgical processing techniques used to promote the formation of refined, homogeneous and contaminant-free structures, favouring improvements in ductility and other properties are discussed. This article further reviews published work on phase constituents, microstructures, alloy developments and mechanical properties of TiAl alloys produced by SPS. Finally, an overview of challenges in as far as the implementation of TiAl in industries of interest are highlighted.
Optimization of Microwave Reductive Roasting Process of Bauxite Residue
Authors: Cardenia Chiara , Balomenos Efthimios , Panias Dimitrios
Published: August 2020

In this study, microwave-assisted heating is presented as a suitable method to transform the hematite and goethite contained in bauxite residue into magnetite, wüstite, and metallic iron, with a short processing time. The final target was the production of a sinter with strong magnetic properties, allowing the magnetic separation of Fe from the residue. The influence of microwave energy on the sample, the effect of irradiation time, and the carbon/bauxite residue mass ratio (C/BR) were the parameters that have been analyzed to optimize the process. Their optimized combination allowed transforming 79% of the iron present in the sinter into metallic iron. However, hercynite was also formed, and the presence of this mineralogical phase could be considered a possible drawback for its magnetic properties.
Force Prediction and Cutting-Parameter Optimization in Micro-Milling Al7075-T6 Based on Response Surface Method
Authors: Zhou Menghua , Chen Yinghua , Zhang Guoqing
Published: August 2020

Optimization of cutting parameters in micro-milling is an important measure to improve surface quality and machining efficiency of the workpiece. Investigation of micro-milling forces prediction plays a positive role in improving machining capacity. To predict micro-milling forces and optimize micro-milling cutting parameters (per-feed tooth (fz), axial cutting depth (ap), spindle speed (n) and tool extended length (l)), a rotatable center composite experiment of micro-milling straight micro-groove in the workpiece of Al7075-T6 were designed, based on second-order response surface methods. According to the experiment results, the least square method was used to estimate the regression coefficient corresponding to the cutting parameters. Simultaneously, the response prediction model of micro-milling was established and successfully coincide the predicted values with the experiment values. The significance of the regression equation was tested by analysis of variance, and the influence of micro-milling cutting parameters on force and top burrs morphology was studied. The experiment results show that in a specific range of cutting parameters, ap and fz have a significant linear relation with the micro-milling force and the top burrs width. According to the optimal response value, the optimized cutting parameters for micro-milling obtained as: n is 11,393 r/min, fz is 6 µm/z, ap is 11 μm and l is 20.8 mm. The research results provide a useful reference for the selection of cutting parameters for micro-milling.
A Novel Electroporation System for Living Cell Staining and Membrane Dynamics Interrogation
Authors: Zhang Yuanjun , Yan Zishen , Xia Xingyu , Lin Yuan
Published: August 2020

A novel electroporation system was developed to introduce transient membrane pores to cells in a spatially and temporally controlled manner, allowing us to achieve fast electrotransfection and live cell staining as well as to systematically interrogate the dynamics of the cell membrane. Specifically, using this platform, we showed that both reversible and irreversible electroporation could be induced in the cell population, with nano-sized membrane pores in the former case being able to self-reseal in ~10 min. In addition, green fluorescent protein(GFP)-vinculin plasmid and 543 phalloidin have been delivered successively into fibroblast cells, which enables us to monitor the distinct roles of vinculin and F-actin in cell adhesion and migration as well as their possible interplay during these processes. Compared to conventional bulk electroporation and staining methods, the new system offers advantages such as low-voltage operation, cellular level manipulation and testing, fast and adjustable transfection/staining and real-time monitoring; the new system therefore could be useful in different biophysical studies in the future.
Targeting Biofilms Therapy: Current Research Strategies and Development Hurdles
Authors: Jiang Yu , Geng Mengxin , Bai Liping
Published: August 2020

Biofilms are aggregate of microorganisms in which cells are frequently embedded within a self-produced matrix of extracellular polymeric substance (EPS) and adhere to each other and/or to a surface. The development of biofilm affords pathogens significantly increased tolerances to antibiotics and antimicrobials. Up to 80% of human bacterial infections are biofilm-associated. Dispersal of biofilms can turn microbial cells into their more vulnerable planktonic phenotype and improve the therapeutic effect of antimicrobials. In this review, we focus on multiple therapeutic strategies that are currently being developed to target important structural and functional characteristics and drug resistance mechanisms of biofilms. We thoroughly discuss the current biofilm targeting strategies from four major aspects—targeting EPS, dispersal molecules, targeting quorum sensing, and targeting dormant cells. We explain each aspect with examples and discuss the main hurdles in the development of biofilm dispersal agents in order to provide a rationale for multi-targeted therapy strategies that target the complicated biofilms. Biofilm dispersal is a promising research direction to treat biofilm-associated infections in the future, and more in vivo experiments should be performed to ensure the efficacy of these therapeutic agents before being used in clinic.
The Quality of Ciders Depends on the Must Supplementation with Mineral Salts
Authors: Tarko Tomasz , Januszek Magdalena , Pater Aneta , Sroka Paweł , Duda-Chodak Aleksandra
Published: August 2020

Providing yeast with the right amount of mineral salts before fermentation can contribute to improving the entire technological process, resulting in a better-quality final product. The aim of this study was to assess the impact of apple must supplementation with mineral salts ((NH4)2SO4, MgSO4, (NH4)3PO4)) on enological parameters, antioxidant activity, total polyphenol content, and the profile of volatile cider compounds fermented with various yeast strains. Rubin cultivar must was inoculated with wine, cider, and distillery or wild yeast strains. Various mineral salts and their mixtures were introduced into the must in doses from 0.167 g/L to 0.5 g/L. The control sample consisted of ciders with no added mineral salts. The basic enological parameters, antioxidant properties, total polyphenol content, and their profile, as well as the composition of volatile compounds, were assessed in ciders. Must supplementation with magnesium salts significantly influenced the use of the analyzed element by yeast cells and was dependent on the yeast strain. In supplemented samples, a decrease in alcohol concentration and total acidity, as well as an increase in the content of extract and total polyphenols, was observed compared to the controls. The addition of ammonium salts caused a decrease in the amount of higher alcohols and magnesium salts, as well as a decrease in the concentration of some esters in ciders.
Antimicrobial O-Alkyl Derivatives of Naringenin and Their Oximes Against Multidrug-Resistant Bacteria
Authors: Duda-Madej Anna , Kozłowska Joanna , Krzyżek Paweł , Anioł Mirosław , Seniuk Alicja , Jermakow Katarzyna , Dworniczek Ewa
Published: August 2020

New antimicrobial agents are needed to address infections caused by multidrug-resistant bacteria. Here, we are reporting novel O-alkyl derivatives of naringenin and their oximes, including novel compounds with a naringenin core and O-hexyl chains, showing activity against clinical strains of clarithromycin-resistant Helicobacter pylori, vancomycin-resistant Enterococcus faecalis, methicillin-resistant Staphylococcus aureus, and beta-lactam-resistant Acinetobacter baumannii and Klebsiella pneumoniae. The minimum inhibitory concentrations (MICs), which provide a quantitative measure of antimicrobial activity, were in the low microgram range for the selected compounds. Checkerboard assays for the most active compounds in combination with antibiotics revealed interactions that varied from synergistic to neutral.
New Deoxyribozymes for the Native Ligation of RNA
Authors: Scheitl Carolin P. M. , Lange Sandra , Höbartner Claudia
Published: August 2020

Deoxyribozymes (DNAzymes) are small, synthetic, single-stranded DNAs capable of catalyzing chemical reactions, including RNA ligation. Herein, we report a novel class of RNA ligase deoxyribozymes that utilize 5′-adenylated RNA (5′-AppRNA) as the donor substrate, mimicking the activated intermediates of protein-catalyzed RNA ligation. Four new DNAzymes were identified by in vitro selection from an N40 random DNA library and were shown to catalyze the intermolecular linear RNA-RNA ligation via the formation of a native 3′-5′-phosphodiester linkage. The catalytic activity is distinct from previously described RNA-ligating deoxyribozymes. Kinetic analyses revealed the optimal incubation conditions for high ligation yields and demonstrated a broad RNA substrate scope. Together with the smooth synthetic accessibility of 5′-adenylated RNAs, the new DNA enzymes are promising tools for the protein-free synthesis of long RNAs, for example containing precious modified nucleotides or fluorescent labels for biochemical and biophysical investigations.
DFT Calculations of 1H- and 13C-NMR Chemical Shifts of Geometric Isomers of Conjugated Linoleic Acid (18:2 ω-7) and Model Compounds in Solution
Authors: Venianakis Themistoklis , Oikonomaki Christina , Siskos Michael G. , Varras Panayiotis C. , Primikyri Alexandra , Alexandri Eleni , Gerothanassis Ioannis P.
Published: August 2020

A density functional theory (DFT) study of the 1H- and 13C-NMR chemical shifts of the geometric isomers of 18:2 ω-7 conjugated linoleic acid (CLA) and nine model compounds is presented, using five functionals and two basis sets. The results are compared with available experimental data from solution high resolution nuclear magnetic resonance (NMR). The experimental 1H chemical shifts exhibit highly diagnostic resonances due to the olefinic protons of the conjugated double bonds. The “inside” olefinic protons of the conjugated double bonds are deshielded than those of the “outside” protons. Furthermore, in the cis/trans isomers, the signals of the cis bonds are more deshielded than those of the trans bonds. These regularities of the experimental 1H chemical shifts of the olefinic protons of the conjugated double bonds are reproduced very accurately for the lowest energy DFT optimized single conformer, for all functionals and basis sets used. The other low energy conformers have negligible effects on the computational 1H-NMR chemical shifts. We conclude that proton NMR chemical shifts are more discriminating than carbon, and DFT calculations can provide a valuable tool for (i) the accurate prediction of 1H-NMR chemical shifts even with less demanding functionals and basis sets; (ii) the unequivocal identification of geometric isomerism of CLAs that occur in nature, and (iii) to derive high resolution structures in solution.
Exploring the Use of Virtual Characters (Avatars), Live Animation, and Augmented Reality to Teach Social Skills to Individuals with Autism
Authors: Kellems Ryan O. , Charlton Cade , Kversøy Kjartan Skogly , Győri Miklós
Published: August 2020

Individuals with autism and other developmental disabilities struggle to acquire and appropriately use social skills to improve the quality of their lives. These critical skills can be difficult to teach because they are context dependent and many students are not motivated to engage in instruction to learn them. The use of multi-modal technologies shows promise in the teaching a variety of skills to individuals with disabilities. iAnimate Live is a project that makes virtual environments, virtual characters (avatars), augmented reality, and animation more accessible for teachers and clinicians. These emerging technologies have the potential to provide more efficient, portable, accessible, and engaging instructional materials to teach a variety of social skills. After reviewing the relevant research on using virtual environments virtual characters (avatars) and animation for social skills instruction, this article describes current experimental applications exploring their use via the iAnimate Live project.
Towards Explainable and Sustainable Wow Experiences with Technology
Authors: Kulzer Manuel , Burmester Michael
Published: August 2020

Interacting with technology can evoke various positive and negative reactions in users. An outstandingly positive user experience enabled by interactive technology is often referred to as a “wow experience” in design practice and research. Such experiences are considered to be emotional, memorable, and highly desirable. Surprisingly, wow experiences have not received much attention in design research. In this study, we try to gain a more in-depth understanding of how wow experiences are caused. Through an exploratory factor analysis, we identify six factors contributing to wow experiences with interactive technology: Hygiene, goal attainment, uniqueness, relevance, emotional fingerprint, and inspiration. We propose an integrated model of wow experience and a prototype questionnaire to measure wow experiences with interactive products based on the identified factors.
The Potential Effects of Probiotics and ω-3 Fatty Acids on Chronic Low-Grade Inflammation
Authors: Hutchinson Ashley N. , Tingö Lina , Brummer Robert Jan
Published: August 2020

Chronic low-grade inflammation negatively impacts health and is associated with aging and obesity, among other health outcomes. A large number of immune mediators are present in the digestive tract and interact with gut bacteria to impact immune function. The gut microbiota itself is also an important initiator of inflammation, for example by releasing compounds such as lipopolysaccharides (LPS) that may influence cytokine production and immune cell function. Certain nutrients (e.g., probiotics, ω-3 fatty acids [FA]) may increase gut microbiota diversity and reduce inflammation. Lactobacilli and Bifidobacteria, among others, prevent gut hyperpermeability and lower LPS-dependent chronic low-grade inflammation. Furthermore, ω-3 FA generate positive effects on inflammation-related conditions (e.g., hypertriglyceridemia, diabetes) by interacting with immune, metabolic, and inflammatory pathways. Ω-3 FA also increase LPS-suppressing bacteria (i.e., Bifidobacteria) and decrease LPS-producing bacteria (i.e., Enterobacteria). Additionally, ω-3 FA appear to promote short-chain FA production. Therefore, combining probiotics with ω-3 FA presents a promising strategy to promote beneficial immune regulation via the gut microbiota, with potential beneficial effects on conditions of inflammatory origin, as commonly experienced by aged and obese individuals, as well as improvements in gut-brain-axis communication.
A Miniature pH Probe Using Functional Microfiber Bragg Grating
Authors: Ran Yang , Xiao Peng , Zhang Yongkang , Hu Deming , Xu Zhiyuan , Liang Lili , Guan Bai-Ou
Published: August 2020

Operando and precisely probing aqueous pH is fundamentally demanded, both in chemical and biological areas. Conventional pH probes, subjected to the larger size, are probably unfit for application in some extreme scenarios, such as a trace amount of samples. In this paper, we have further developed the pH sensor that leverages the microfiber Bragg grating with an ultra-compact size down to an order of magnitude of 10−14 m3. Using the electrostatic self-assembly layer-by-layer technique, the functional film consisting of sodium alginate, which harnesses a pH-dependent hygroscopicity, is immobilized on the fiber surface. Consequently, the alteration of aqueous pH could be quantitatively indicated by the wavelength shift of the grating resonance via the refractive index variation of the sensing film due to the water absorption or expulsion. The grating reflections involving fundamental mode and higher order mode exhibit the sensitivities of −72 pm/pH and −265 pm/pH, respectively. In addition, temperature compensation can be facilitated by the recording of the two reflections simultaneously. Furthermore, the modeling and simulation results predict the pivotal parameters of the configuration in sensitivity enhancement. The proposed proof-of-concept enriches the toolbox of pH sensor for catering to the need of detection in some extremely small spaces—for example, the living cells or the bio-tissues.
Effect of Ethanol Extracts of Propolis (EEPs) against Staphylococcal Biofilm—Microscopic Studies
Authors: Grecka Katarzyna , Xiong Zirui Ray , Chen Hanyu , Pełka Karolina , Worobo Randy W. , Szweda Piotr
Published: August 2020

Staphylococci growing in the form of biofilm exhibit high resistance to a plethora of antibiotics. The aim of the study was to assess the influence of ethanolic extract of propolis (EEPs) on S. epidermidis ATCC 35984 biofilm using fluorescent microscopy. Propidium iodide (PI) and SYTO 9 were used for differentiation of live and dead cells, and calcofluor white was used to stain the extracellular matrix, the self-produced extracellular polymeric substances (EPS). The outcomes of the research confirm the promising potential of EEPs for eradication of staphylococcal biofilm. However, its activity cannot be classified as fully satisfactory, either in terms of the effectiveness of elimination of bacterial cells or disturbing the EPS structure. A two or even four times higher concentration of EEPs compared to MIC (Minimum Inhibitory Concentration) against planktonic cells (128 µg/mL) was necessary for effective (estimated for 90%) elimination of living cells from the biofilm structure. Unfortunately, even at that concentration of EEPs, the extracellular matrix was only partially disturbed and effectively protected the residual population of living cells of S. epidermidis ATCC 35984. In our opinion, a combination of EEPs with agents disrupting components of EPS, e.g., proteases, lysines, or enzymes degrading extracellular DNA or PIA (polysaccharide intercellular adhesin).