bluuebunny/crossref_metadata_2025 huggingface dataset:
High incidence of atrial fibrillation in patients treated with ibrutinib
Authors: Baptiste Florian , Cautela Jennifer , Ancedy Yan , Resseguier Noémie , Aurran Thérèse , Farnault Laure , Escudier Marion , Ammar Chloé , Gaubert Mélanie , Dolladille Charles , Barraud Jeremie , Peyrol Michael , Cohen Ariel , Paganelli Franck , Alexandre Joachim , Ederhy Stephane , Thuny Franck
Published: May 2019

Objective

Atrial fibrillation (AF) is one of the most common side effects of ibrutinib, a drug that has dramatically improved the prognosis of chronic B-cell malignancies such as chronic lymphocytic leukaemia (CLL). The true incidence of ibrutinib-related AF (IRAF) is not well known and its therapeutic management poses unique challenges especially due to the inherent risk of bleeding. We aimed to determine the incidence and predictors of IRAF, and to analyse its management and outcome.

Methods

A standardised monitoring was applied at two cardio-oncology clinics in consecutive patients referred before and during ibrutinib therapy. The primary endpoint was the incidence of IRAF. The excess of AF incidence with ibrutinib was studied by comparing the incidence of IRAF with the expected incidence of AF in general population and in patients with CLL not exposed to ibrutinib.

Results

53 patients were included. The incidence of IRAF was 38% at 2 years and the risk was 15-fold higher than the AF risk in both the general population and patients with CLL not exposed to ibrutinib (p<0.0001). The majority of cases occurred in asymptomatic patients within the first 6 months. Left atrial volume index ≥40 mL/m2at treatment initiation identified patients at high risk of developing IRAF. No major bleeding events occurred in patients on ibrutinib, although the majority of patients with IRAF were treated with anticoagulants.

Conclusions

This cardio-oncology study showed that the risk of IRAF was much higher than previously reported. The majority of cases occurred in asymptomatic patients justifying close monitoring.
Estimating the size of key populations for HIV in Singapore using the network scale-up method
Authors: Teo Alvin Kuo Jing , Prem Kiesha , Chen Mark I C , Roellin Adrian , Wong Mee Lian , La Hanh Hao , Cook Alex R
Published: May 2019

Objectives

To develop a localised instrument and Bayesian statistical method to generate size estimates—adjusted for transmission error and barrier effects—of at-risk populations in Singapore.

Methods

We conducted indepth interviews and focus group to guide the development of the survey questionnaire. The questionnaire was administered between July and August 2017 in Singapore. Using the network scale-up method (NSUM), we developed a Bayesian hierarchical model to estimate the number of individuals in four hidden populations at risk of HIV. The method accounted for both transmission error and barrier effects using social acceptance measures and demographics.

Results

The adjusted size estimate of the population of male clients of female sex workers was 72 000 (95% CI 51 000 to 100 000), of female sex workers 4200 (95% CI 1600 to 10 000), of men who have sex with men 210 000 (95% CI 140 000 to 300 000) and of intravenous drug users 11 000 (95% CI 6500 to 17 000).

Conclusions

The NSUM with adjustment for attitudes and demographics allows national-level estimates of multiple priority populations to be determined from simple surveys of the general population, even in relatively conservative societies.
Changes in roll-your-own tobacco and cigarette sales volume and prices before, during and after plain packaging legislation in the UK
Authors: Opazo Breton Magdalena , Britton John , Bogdanovica Ilze
Published: May 2019

Background

Plain packaging and minimum pack size legislation for tobacco products was introduced in the UK in May 2016, with a 1-year sell-off period until May 2017, during which both fully branded and plain packs of various sizes were legally available. This study investigates trends in prices of roll-your-own tobacco (RYO) before, during and after implementation of this legislation, and compares trends with those observed in the cigarette market.

Methods

We used Nielsen Scantrack data for the period from March 2013 to June 2018 to describe trends in UK inflation-adjusted prices and volumes of both RYO and cigarettes, and linear regression to estimate changes in prices associated with the introduction of plain packaging and the minimum pack sizes of 30 g RYO and 20 cigarettes.

Results

In contrast to a downward trend in cigarette sales volumes, RYO volumes rose throughout the study period. By the time plain packs accounted for 75% or more of sales, the average price of products sold in equivalent pack sizes had increased, relative to average prices in the year before implementation and with adjustment for tax changes, from 34.9 to 38.8 pence per gram for RYO (mean difference 4.26, 95% CI 3.99 to 4.53 pence, 12% increase), and from 38.6 to 41.13 pence for cigarettes (mean difference 2.53, 95% CI 2.24 to 2.83 pence, 7% increase) per cigarette.

Conclusions

New legislation resulted in higher prices for RYO and manufactured cigarettes. However, sales volumes of RYO continued to increase throughout the study period, perhaps because RYO remains a less expensive means of smoking tobacco.
The role of sex and temperature in melanin-based immune function
Authors: Ehrlich Rebecca L. , Zuk Marlene
Published: May 2019

Sex differences in immunity have been observed across a wide range of species. Still, it remains unclear how sex-specific interactions with the environment are linked to sex differences in immunity. We studied the plasticity of immunological sex differences by focusing on melanin-based traits in the Pacific field cricket (Teleogryllus oceanicus (Le Guillou, 1841)). Insects rely on the pigment melanin for both immune function and coloration of the cuticle; therefore, changes in melanin production for one of these traits may indirectly affect the other. Male crickets use melanized wing structures to chirp. These cuticular structures are missing in females and a songless male morph. Given that the thermal environment influences cuticle melanization, we investigated the interactive effects of sex and developmental temperature on melanin-based immunity. Both immunity and wing cuticle melanism were reduced in individuals that developed under warmer temperatures. Rearing temperature also mediated the extent to which the sexes differed in immune traits. Males had darker cuticles, whereas females expressed greater immune activity, suggesting that sex-specific investment in melanin corresponds with sex differences in immunity. However, the lack of immunological differences between the two male morphs does not support the hypothesis that investment in cuticle melanism affects investment in immunity.
Recommended best practices for plastic and litter ingestion studies in marine birds: Collection, processing, and reporting
Authors: Provencher Jennifer F. , Borrelle Stephanie B. , Bond Alexander L. , Lavers Jennifer L. , van Franeker Jan A. , Kühn Susanne , Hammer Sjúrður , Avery-Gomm Stephanie , Mallory Mark L.
Published: May 2019

Marine plastic pollution is an environmental contaminant of significant concern. There is a lack of consistency in sample collection and processing that continues to impede meta-analyses and large-scale comparisons across time and space. This is true for most taxa, including seabirds, which are the most studied megafauna group with regards to plastic ingestion research. Consequently, it is difficult to evaluate the impacts and extent of plastic contamination in seabirds fully and accurately, and to make inferences about species for which we have little or no data. We provide a synthesized set of recommendations specific for seabirds and plastic ingestion studies that include best practices in relation to sample collection, processing, and reporting, as well as highlighting some “cross-cutting” methods. We include guidance for how carcasses, regurgitations, and pellets should be handled and treated to prevent cross-contamination, and a discussion of what size class of microplastics can be assessed in each sample type. Although we focus on marine bird samples, we also include standardized techniques to remove sediment and biological material that are generalizable to other taxa. Lastly, metrics and data presentation of ingested plastics are briefly reviewed in the context of seabird studies.
Fusion of Underwater Image Enhancement and Restoration
Authors: Sethi Rajni , Indu Sreedevi
Published: May 2019

Optical properties of water distort the quality of underwater images. Underwater images are characterized by poor contrast, color cast, noise and haze. These images need to be pre-processed so as to get some information. In this paper, a novel technique named Fusion of Underwater Image Enhancement and Restoration (FUIER) has been proposed which enhances as well as restores underwater images with a target to act on all major issues in underwater images, i.e. color cast removal, contrast enhancement and dehazing. It generates two versions of the single input image and these two versions are fused using Laplacian pyramid-based fusion to get the enhanced image. The proposed method works efficiently for all types of underwater images captured in different conditions (turbidity, depth, salinity, etc.). Results obtained using the proposed method are better than those for state-of-the-art methods.
Symmetries of Hamiltonians describing systems with arbitrary spins
Authors: Cervia Michael J. , Patwardhan Amol V. , Balantekin A. B.
Published: May 2019

We consider systems where dynamical variables are the generators of the SU(2) group. A subset of these Hamiltonians is exactly solvable using the Bethe ansatz techniques. We show that Bethe ansatz equations are equivalent to polynomial relationships between the operator invariants, or equivalently, between eigenvalues of those invariants.
Reliability Analysis of a System with Multiple-Stage Policy Considering Type I and II Errors
Authors: Imaizumi Mitsuhiro , Kimura Mitsutaka
Published: May 2019

This paper formulates a stochastic model for a system with illegal access. The server has the function of IDS, and illegal access is checked in multiple stages which consist of simple check and detailed check. In this model, we consider type I and II errors of simple check and a type I error of detailed check. There are two cases where IDS judges the occurrence of illegal access erroneously. One is when illegal access does not occur, and the other is when illegal access occurs. We apply the theory of Markov renewal processes to a system with illegal access, and derive the mean time and the expected checking number until a server system becomes faulty. Further, an optimal policy which minimizes the expected cost is discussed. Finally, numerical examples are given.
Deep Belief Network with Seasonal Decomposition for Solar Power Output Forecasting
Authors: Wang Ching-Hsin , Lin Kuo-Ping , Lu Yu-Ming , Wu Chih-Feng
Published: May 2019

Solar power is a type of renewable energy system that uses solar energy to produce electricity, and is regarded as one of the most important power sources in Taiwan. Since sunshine duration affects the amount of energy that can be generated by a solar power, the seasons of the year are important factors that should be considered for accurate solar power prediction. In the last decade, the use of artificial intelligence for forecasting systems have been quite popular, and the deep belief network (DBN) models started getting more attention. In this study, a seasonal deep belief network (SDBN) was developed to forecast monthly solar power output data. The SDBN was constructed by combining seasonal decomposition method and DBN. Further, this study used monthly solar power output data from the Taiwan Power Company. The results indicated that the proposed forecasting system demonstrated a superior performance in terms of forecasting accuracy. Also, the performance of autoregressive integrated moving average (ARIMA), generalized regression neural network (GRNN), and DBN obtained from a separate study were compared to the performance of the proposed SDBN model and showed that the latter was better than the other three models. Thus, the SDBN model can be used as an alternative method for monthly solar power output data forecasting.
CREDIT SPREAD AND LIQUIDATION VALUE-BASED DEBT FINANCING CONSTRAINT
Authors: SHIBATA TAKASHI , NISHIHARA MICHI
Published: May 2019

This study examines the corporate credit spread of a firm that is constrained by an upper limit of debt issuance depending on the liquidation (collateral) value. We provide four important results. First, the upper limit of debt issuance may induce the firm to issue riskless (risk-free) debt. Second, the upper limit decreases the credit spread. Third, the credit spread increases with the liquidation value when the firm is financially constrained, while the credit spread decreases with the liquidation value when it is not. Fourth, the firm is likely to be financially constrained when cash flow volatility is high, the risk-free interest rate is low, corporate tax is high, and bankruptcy cost is high. Our results fit well with empirical studies.
Numerical Modeling of Free-Surface Wave Effects on Flexural Vibration of Floating Structures
Authors: Sepehrirahnama Shahrokh , Ong Eng Teo , Lee Heow Pueh , Lim Kian Meng
Published: May 2019

To investigate flexural vibration of structures in a fluid, a numerical algorithm was developed to relate the added mass and damping effects of the fluid to each mode of vibration. These are separate from the traditional added mass associated with rigid body motion, such as the translational motion along Cartesian axes. In this formulation, small-amplitude free surface waves were accounted for by using a nonsingular implementation of the free-surface Green’s function for a potential flow solver based on Boundary Element Method. The formulation was applied to the forced vibration of structures, namely, a hemispherical shell and a reinforced half cylinder with typical dimensions of ships and offshore structures, to obtain their dynamic response at various excitation frequencies. It is observed that resonance frequency of the structure, in contact with water, decreases due to the added mass effect. The influence of the free-surface wave on the fluid loading was investigated for large structures. It is simpler to relate the fluid added mass to mode shapes rather than distribution of fluid load over wetted surface of the structure in engineering simulations. Moreover, it is found that the vibration energy radiated away by the fluid surface wave has little influence on the vibration response of the shell structures.
The Roles of a Matricellular CCN Family Protein CCN5 in Cardiac Fibrosis of Heart Failure
Authors: Liu Jie , Zhuang Tao , Chen Xiaoli , Zhang Lin , Zhang Yuzhen
Published: May 2019

Cardiac fibrosis is a major player in cardiovascular disease, both as a contributor to the development of disease and a post-injury response that drives progression to heart failure. Despite the identification of many mechanisms responsible for cardiovascular fibrosis, such as angiotensin II, TGF-[Formula: see text] and endothelin-1, to date no treatments have emerged that have effectively reduced the excess deposition of extracellular matrix associated with fibrotic conditions. Matricellular CCN proteins spatiotemporally regulated nonstructural components of the extracellular matrix (ECM) and participated in many essential biological functions, including wound healing and fibrotic diseases. CCN5 exhibited the opposing effects of CCN2 on the development of cardiac hypertrophy and fibrosis, and overexpression of matricellular protein CCN5 in the heart by adenoviral deliver significantly improved cardiac fibrosis in severe heart failure. Future time- and cell-specific study of CCN5 effect and its domain-specific function on fibrotic development and progression will advance our understanding of cardiac fibrosis, and meanwhile provide opportunities for therapeutic intervention of heart failure.
Thermoelectric Air-Conditioning System: Building Applications and Enhancement Techniques
Authors: Baheta Aklilu Tesfamichael , Looi Kar Kin , Oumer Ahmed Nurye , Habib Khairul
Published: May 2019

The high reliability, the absence of working fluid and auxiliary pipes in the thermoelectric cooling application have attracted the attention of researchers in the last two decades. However, the use of thermoelectric air-conditioning system for building application has not been entirely explored due to its low coefficient of performance (COP) compared to the conventional air conditioning system. To overcome this primary limitation, different COP enhancement techniques of thermoelectric for air conditioning system building application are made available. This paper provides the recent development of thermoelectric air conditioning system in building applications, such as thermoelectric radiant panel ceiling, thermoelectric air duct system and thermoelectric cooling facades. It also provides the different strategies to enhance its performance in order to fit this technology in real building applications such as the integration of water-cooling system, phase change materials, evaporator cooling system and nanofluid micro-channel heat sinks. Lastly, the challenges of thermoelectric air-conditioning systems and future research directions are discussed.
Exploration in an Extreme Underwater Environment
Authors: McLaren Alfred Scott
Published: May 2019

“Exploration of Extreme Underwater Environments” briefly recounts the highlights of the author’s experiences during the exploration of an extreme underwater environment: As commander of the nuclear attack submarine, USS Queenfish (SSN 651) during the historic first survey of 5,200 km of the entire uncharted, sea-ice covered, Siberian Continental Shelf during the summer of 1970.
Electronics Supply Chain Integrity Enabled by Blockchain
Authors: Xu Xiaolin , Rahman Fahim , Shakya Bicky , Vassilev Apostol , Forte Domenic , Tehranipoor Mark
Published: May 2019

Electronic systems are ubiquitous today, playing an irreplaceable role in our personal lives, as well as in critical infrastructures such as power grids, satellite communications, and public transportation. In the past few decades, the security of software running on these systems has received significant attention. However, hardware has been assumed to be trustworthy and reliable “by default” without really analyzing the vulnerabilities in the electronics supply chain. With the rapid globalization of the semiconductor industry, it has become challenging to ensure the integrity and security of hardware. In this article, we discuss the integrity concerns associated with a globalized electronics supply chain. More specifically, we divide the supply chain into six distinct entities: IP owner/foundry (OCM), distributor, assembler, integrator, end user, and electronics recycler, and analyze the vulnerabilities and threats associated with each stage. To address the concerns of the supply chain integrity, we propose a blockchain-based certificate authority framework that can be used to manage critical chip information such as electronic chip identification, chip grade, and transaction time. The decentralized nature of the proposed framework can mitigate most threats of the electronics supply chain, such as recycling, remarking, cloning, and overproduction.
Comparison of IOP Measurement by Goldmann Applanation Tonometer, ICare Rebound Tonometer, and Tono-Pen in Keratoconus Patients after MyoRing Implantation
Authors: Rateb Mahmoud , Abdel-Radi Mahmoud , Eldaly Zeiad , Elmohamady Mohamed Nagy , Noor El Din Asaad
Published: May 2019

Purpose. To evaluate the different IOP readings by Goldmann applanation tonometer (GAT), ICare rebound tonometer, and Tono-Pen in keratoconus patients after MyoRing implantation. To assess the influence of central corneal thickness (CCT) and thinnest corneal location (TCL) on IOP measurements by different tonometers. Setting. Prospective observational study was conducted in two private centers in Egypt from February 2015 to November 2016. Methods. Seventeen eyes of 10 patients suffering from keratoconus and who underwent MyoRing implantation were recruited. All subjects underwent GAT, ICare, and Tono-Pen IOP measurements in random order. Central corneal thickness and thinnest corneal location were assessed by Pentacam. Difference in mean in IOP readings was assessed by T-test. Correlation between each pair of devices was evaluated by Pearson correlation coefficient. The Bland–Altman analysis was used to assess intertonometer agreement. Results. Seventeen eyes (10 patients) were evaluated. The mean IOP reading was 13.9 ± 3.68, 12.41 ± 2.87, and 14.29 ± 1.31 mmHg in GAT, ICare, and Tono-Pen group, respectively. There was a significant difference between IOP readings by GAT/ICare and Tono-Pen/ICare (p value: 0.032 and 0.002, respectively) with no significant difference between GAT/Tono-Pen (p value: 0.554). Mean difference in IOP measurements between GAT/ICare was 1.49 ± 2.61 mmHg, Tono-Pen/ICare was 1.89 ± 2.15 mmHg, and GAT/Tono-Pen was −0.39 ± 2.59 mmHg. There was no significant correlation between the difference in IOP readings among any pair of devices and CCC or TCL. The Bland–Altman analysis showed a reasonable agreement between any pair of tonometers.
Evaluation of the Dislodgement Resistance of a New Pozzolan-Based Cement (EndoSeal MTA) Compared to ProRoot MTA and Biodentine in the Presence and Absence of Blood
Authors: Adl Alireza , Sadat Shojaee Nooshin , Pourhatami Negar
Published: May 2019

Introduction. This in vitro study investigated the dislodgement resistance of EndoSeal MTA, a new pozzolan-containing calcium silicate-based material, in comparison with ProRoot MTA and Biodentine in the presence and absence of contamination with blood. Methods. Standard furcal perforations were created in 180 human mandibular first molars. The teeth were randomly allocated to 12 groups of 15 each. ProRoot MTA, Biodentine, and EndoSeal MTA were used to repair the perforations. In half of the samples, the walls of the perforated areas were contaminated with blood, whereas saline was injected into the other half. A push-out test was performed using a universal testing machine after 24 hours or 7 days. To evaluate failure patterns, the samples were split into half and were examined under a stereomicroscope at a 20x magnification. Data were analyzed using three-way analysis of variance, Tukey test, and Student’s t-test. Results. At both time intervals and in the presence and absence of contamination with blood, ProRoot MTA and Biodentine had significantly higher retention values than EndoSeal MTA (p<0.001). Contamination with blood had no effect on EndoSeal MTA; however, it negatively affected the dislodgement resistance of Biodentine at 24 hours and ProRoot MTA at both time intervals (p<0.05). Time significantly affected only the bond strength of the uncontaminated groups (p>0.001). The most common type of failure was mixed for ProRoot MTA and Biodentine, whereas it was cohesive for EndoSeal MTA. Conclusions. ProRoot MTA and Biodentine showed higher values of bond strength than EndoSeal MTA and may thus be better options for the repair of root perforations.
Structure and Biological Properties of Surface-Engineered Carbon Nanofibers
Authors: Smolka Wojciech , Panek Agnieszka , Gubernat Maciej , Szczypta-Fraczek Aneta , Jelen Piotr , Paluszkiewicz Czeslawa , Markowski Jaroslaw , Blazewicz Marta
Published: May 2019

The aim of this work was to manufacture, using the electrospinning technique, polyacrylonitrile- (PAN-) based carbon nanofibers in the form of mats for biomedical applications. Carbon nanofibers obtained by carbonization of the PAN nanofibers to 1000°C (electrospun carbon nanofibers (ECNF)) were additionally oxidized in air at 800°C under reduced pressure (electrospun carbon nanofibers oxidized under reduced pressure (ECNFV)). The oxidative treatment led to partial removal of a structurally less-ordered carbon phase from the near-surface region of the carbon nanofibers. Both types of carbon fibrous mats were studied using scanning electron microscopy (SEM), high-resolution transmission electron microscopy (TEM), XRD, and Raman spectroscopy. The morphology, microstructure, and surface properties of both materials were analyzed. The oxidative treatment of carbon nanofibers significantly changed their surface morphology and physical properties (wettability, surface electrical resistance). Biological tests (genotoxicity, fibroblast, and human osteoblast-like MG63 cultures) were carried out in contact with both materials. Genotoxicity study conducted by means of comet assays revealed significant differences between both carbon nanofibers. Fibroblasts contacted with the as-received carbon nanofibers (ECNF) showed a significantly higher level of DNA damage compared to control and oxidized carbon nanofibers (ECNFV). The ECNFV nanofibers were not cytotoxic, whereas ECNF nanofibers contacted with both types of cells indicated a cytotoxic effect. The ECNFV introduced into cell culture did not affect the repair processes in the cells contacting them.
Human Immune System Increases Breast Cancer-Induced Osteoblastic Bone Growth in a Humanized Mouse Model without Affecting Normal Bone
Authors: Kähkönen Tiina E. , Suominen Mari I. , Mäki-Jouppila Jenni H. E. , Halleen Jussi M. , Tanaka Azusa , Seiler Michael , Bernoulli Jenni
Published: May 2019

Bone metastases are prevalent in many common cancers such as breast, prostate, and lung cancers, and novel therapies for treating bone metastases are needed. Human immune system-engrafted models are used in immuno-oncology (IO) studies for subcutaneous cancer cell or patient-derived xenograft implantations that mimic primary tumor growth. Novel efficacy models for IO compounds on bone metastases need to be established. The study was performed using CIEA NOG (NOG) mice engrafted with human CD34+ hematopoietic stem cells (huNOG) and age-matched immunodeficient NOG mice. Bone phenotyping was performed to evaluate baseline differences. BT-474 human breast cancer cells were inoculated into the tibia bone marrow, and cancer-induced bone changes were monitored by X-ray imaging. Bone content and volume were analyzed by dual X-ray absorptiometry and microcomputed tomography. Tumor-infiltrating lymphocytes (TILs) and the expression of immune checkpoint markers were analyzed by immunohistochemistry. Bone phenotyping showed no differences in bone architecture or volume of the healthy bones in huNOG and NOG mice, but the bone marrow fat was absent in huNOG mice. Fibrotic areas were observed in the bone marrow of some huNOG mice. BT-474 tumors induced osteoblastic bone growth. Bone lesions appeared earlier and were larger, and bone mineral density was higher in huNOG mice. huNOG mice had a high number of human CD3-, CD4-, and CD8-positive T cells and CD20-positive B cells in immune-related organs. A low number of TILs and PD-1-positive cells and low PD-L1 expression were observed in the BT-474 tumors at the endpoint. This study reports characterization of the first breast cancer bone growth model in huNOG mice. BT-474 tumors represent a “cold” tumor with a low number of TILs. This model can be used for evaluating the efficacy of combination treatments of IO therapies with immune-stimulatory compounds or therapeutic approaches on bone metastatic breast cancer.
Impact of Moderate Sedation versus Monitored Anesthesia Care on Outcomes and Cost of Endobronchial Ultrasound Transbronchial Needle Aspiration
Authors: Boujaoude Ziad , Arya Rohan , Shrivastava Aseem , Pratter Melvin , Abouzgheib Wissam
Published: May 2019

Background and Objectives. The ideal type of sedation for endobronchial ultrasound transbronchial needle aspiration (EBUS-TBNA) is not known. Two previous studies comparing the diagnostic yield between moderate sedation (MS) and deep sedation/general anesthesia (DS/GA) had provided conflicting results with one study clearly favoring the latter. No study had addressed cost. This is concerning for pulmonologists without routine access to anesthesia services. Our objective was to assess the impact of MS and Monitored Anesthesia Care (sedation administered and monitored by an anesthesiologist) on the outcomes and cost of EBUS-TBNA. Materials and Methods. We performed a retrospective review of prospectively collected data on consecutive EBUS-TBNA performed under two different types of sedation in a single academic center. A diagnostic TBNA was defined as an aspirate yielding any specific diagnosis or if subsequent surgery or follow-up of nondiagnostic/normal aspirates showed no pathology. Current Medicare time-based allowances were used for professional charges calculation. Results. There was no difference observed between MS and MAC in regards of the diagnostic yield (92.9% versus 91.9%), procedure duration, number, location, and size of lymph node (LN) sampled, but there were more passes per LN with MAC. The average charges were 74.30 USD for MS and 319.91 for MAC. There were more hypotensive and desaturations episodes with MAC but none required escalation of care. Conclusions. When performed under MS, EBUS-TBNA has similar diagnostic yield as under MAC but may be associated with less side effects. The difference in sedation cost is modest; however, an additional 245$ for each EBUS done under MAC would have significant cost implications on the health system. These findings are of critical importance for bronchoscopists without routine access to anesthesia services and for optimization of healthcare cost and resource utilization.
Seismic Strength and Stiffness for Recycled Aggregate Concrete‐Filled Steel Tube Frame
Authors: Zhang Xianggang , Yang Jianhui , Zhang Yaozong , Gao Xiang
Published: May 2019

To study the seismic strength and stiffness for recycled aggregate concrete‐filled steel tube (RACFST) frame, two‐frame specimens made up of RACFST column and reinforced recycled aggregate concrete (RAC) beam were used for a seismic test under reversed low‐cycle loading. The failure mechanism, hysteresis curve, strength attenuation, and stiffness degradation were determined for the specimens. The design methods for the story shear bearing capacity and stiffness for the single‐layer single‐span RACFST frame were discussed. It is shown that the seismic design requirements including “strong column weak beam” and “strong shear weak bending” can be met. The hysteresis curves are symmetric and the strength attenuation and rigidity degeneration change significantly, then change a little, and then significantly again under the same displacement. It is possible that the methods including elastic bending moment at the column end, plastic hinge at the column end, and plastic hinge at the column bottom can all be applied to the design calculation of the story shear bearing capacity for the single‐layer single‐span RACFST frame. The method adopted in this paper can be used to estimate the original elastic layer stiffness of the RACFST frame.
Steady‐State Analysis and Output Voltage Minimization Based Control Strategy for Electric Springs in the Smart Grid with Multiple Renewable Energy Sources
Authors: Zou Yun , Chen Michael Z. Q. , Hu Yinlong , Zou Yun
Published: May 2019

This paper presents a general steady‐state analysis and proposes a minimal compensating voltage (MCV) control scheme for the second generation of electric springs (ES‐2) in the power system with substantial penetration of intermittent renewable energy sources. For the steady‐state analysis, the relationship among the fluctuating part of the supply voltage, the voltage at the point of common‐coupling (PCC), and the compensating voltage provided by ES‐2 is derived, which implies that the phase angle related to the PCC voltage can be used as a degree of freedom for the control design to obtain a minimal compensating voltage in a given system. Such a fact is utilized in the control design to obtain the reference of PCC voltage by tuning the above‐mentioned phase angle. Once the phase angle of the PCC voltage is chosen, the maximal compensating voltage can be estimated based on the fluctuating part of the supply voltage which can be estimated a priori. Such a fact can be used to design suitable electric springs with appropriate compensating capacity to avoid overcapacity. Numerical simulations are conducted to verify the effectiveness of the steady‐state analysis and the proposed control scheme for ES‐2.
Biocompatibility of Developing 3D-Printed Tubular Scaffold Coated with Nanofibers for Bone Applications
Authors: Vazquez-Vazquez Febe Carolina , Chanes-Cuevas Osmar Alejandro , Masuoka David , Alatorre Jesús Arenas , Chavarria-Bolaños Daniel , Vega-Baudrit José Roberto , Serrano-Bello Janeth , Alvarez-Perez Marco Antonio
Published: May 2019

3D printing with controlled microarchitectures has gained traction in a wide variety of fields, including bone tissue engineering, because it represents an exciting alternative for the synthesis of new scaffolds due to its rapid manufacturing process, high precision, cost-effectiveness, and ease of use. Thus, this study is aimed at evaluating the biocompatibility response of a 3D-printed tubular scaffold coated by a layer of 7% PLA nanofibers. The morphology, structure, and chemical composition of the 3D-printed tubular scaffold were characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD), Fourier Transform Infrared (FTIR), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), and surface property analysis by profilometry. The biocompatibility response of the scaffold was assessed by cell adhesion, proliferation, and cell-material interactions of human fetal osteoblasts. Our results showed that 3D printing allowed obtaining similar and reproducible structures and the biocompatibility assays showed that nanofiber coating of the surface of the 3D tubular scaffold promoted an improvement on cell attachment, proliferation, and the morphology of osteoblast cells when compared with a noncoated scaffold. In conclusion, the surface of the 3D-printed tubular scaffold could be improved by the deposition of a nanofiber layer to render a more mimetic and active topography with excellent cellular biocompatibility for bone tissue applications.
Systemic Inflammation Impairs Mood Function by Disrupting the Resting-State Functional Network in a Rat Animal Model Induced by Lipopolysaccharide Challenge
Authors: Zhu Xia , Ji Mu-Huo , Li Shu-Ming , Li Bin , Mei Li , Yang Jian-Jun
Published: May 2019

Background. Systemic inflammation impairs cognitive performance, yet the brain networks mediating this process remain to be elucidated. The purpose of the current study was to use resting-state functional magnetic resonance imaging (fMRI) to explore changes in the functional connectivity in a lipopolysaccharide- (LPS-) induced systemic inflammation animal model. Materials and Methods. We used the regional homogeneity (ReHo) method to examine abnormal brain regions between the control and LPS groups and then considered them as seeds of functional connectivity analysis. Results. Compared with the control group, our study showed that (1) LPS impaired mood function, as reflected by a depression-like behavior in the forced swim test; (2) LPS induced significantly increased ReHo values in the anterior cingulate cortex (ACC) and caudate putamen (CPu); (3) the ACC seed showed increased functional connectivity with the retrosplenial cortex, superior colliculus, and inferior colliculus; and (4) the right CPu seed showed increased functional connectivity with the left CPu. Linear regression analysis showed a LPS-induced depression-like behavior which was associated with increased ReHo values in the ACC and right CPu. Moreover, the LPS-induced depression-like behavior was related to increased functional connectivity between the right CPu and left CPu. Conclusion. This is the first study to show that systemic inflammation impairs mood function that is associated with an altered resting-state functional network based on ReHo analysis, providing evidence of the abnormal regional brain spontaneous activity which might be involved in inflammation-related neurobehavioral abnormalities.
Mitophagy and Oxidative Stress in Cancer and Aging: Focus on Sirtuins and Nanomaterials
Authors: Vernucci Enza , Tomino Carlo , Molinari Francesca , Limongi Dolores , Aventaggiato Michele , Sansone Luigi , Tafani Marco , Russo Matteo A.
Published: May 2019

Mitochondria are the cellular center of energy production and of several important metabolic processes. Mitochondrion health is maintained with a substantial intervention of mitophagy, a process of macroautophagy that degrades selectively dysfunctional and irreversibly damaged organelles. Because of its crucial duty, alteration in mitophagy can cause functional and structural adjustment in the mitochondria, changes in energy production, loss of cellular adaptation, and cell death. In this review, we discuss the dual role that mitophagy plays in cancer and age-related pathologies, as a consequence of oxidative stress, evidencing the triggering stimuli and mechanisms and suggesting the molecular targets for its therapeutic control. Finally, a section has been dedicated to the interplay between mitophagy and therapies using nanoparticles that are the new frontier for a direct and less invasive strategy.
In Vitro Anti-Inflammatory Effect ofSalvia sagittataEthanolic Extract on Primary Cultures of Porcine Aortic Endothelial Cells
Authors: Tubon Irvin , Zannoni Augusta , Bernardini Chiara , Salaroli Roberta , Bertocchi Martina , Mandrioli Roberto , Vinueza Diego , Antognoni Fabiana , Forni Monica
Published: May 2019

The aim of the present research was to study the effects of an ethanolic extract ofSalvia sagittataRuiz & Pav (SSEE), an endemic Ecuadorian plant traditionally used to treat inflammation and different intestinal affections, on primary cultures of porcine aortic endothelial cells (pAECs). pAECs were cultured in the presence of different concentrations (1-200 μg/mL) of SSEE for 24 h, and cytotoxicity was evaluated by the MTT assay. SSEE did not negatively affect cellular viability at any concentration tested. Cell cycle was analyzed and no significant change was observed. Then, the anti-inflammatory effects of SSEE on pAECs were analyzed using a lipopolysaccharide (LPS) as the inflammatory stimulus. Different markers involved in the inflammatory process, such as cytokines and protective molecules, were evaluated by real-time quantitative PCR and Western blot. SSEE showed the ability to restore pAEC physiological conditions reducing interleukin-6 and increasing Heme Oxygenase-1 protein levels. The phytochemical composition of SSEE was also evaluated via HPLC-DAD and spectrophotometric assays. The presence of different phenolic acids and flavonoids was revealed, with rosmarinic acid as the most abundant component. SSEE possesses an interesting antioxidant activity, as assessed through both the Oxygen Radical Absorbance Capacity (ORAC) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) assays. In conclusion, results suggest that SSEE is endowed with anin vitroanti-inflammatory effect. This represents the initial step in finding a possible scientific support for the traditional therapeutic use of this plant.
Chronic Alcohol Exposure Induced Neuroapoptosis: Diminishing Effect of Ethyl Acetate Fraction from Aralia elata
Authors: Kwon Bong Seok , Kim Jong Min , Park Seon Kyeong , Kang Jin Yong , Kang Jeong Eun , Lee Chang Jun , Park Sang Hyun , Park Su Bin , Yoo Seul Ki , Lee Uk , Kim Dae-Ok , Heo Ho Jin
Published: May 2019

An ethyl acetate fraction from Aralia elata (AEEF) was investigated to confirm its neuronal cell protective effect on ethanol-induced cytotoxicity in MC-IXC cells and its ameliorating effect on neurodegeneration in chronic alcohol-induced mice. The neuroprotective effect was examined by methylthiazolyldiphenyl-tetrazolium bromide (MTT) and 2′,7′-dichlorodihydrofluorescein diacetate (DCF-DA) assays. As a result, AEEF reduced alcohol-induced cytotoxicity and oxidative stress. To evaluate the improvement of learning, memory ability, and spatial cognition, Y-maze, passive avoidance, and Morris water maze tests were conducted. The AEEF groups showed an alleviation of the decrease in cognitive function in alcohol-treated mice. Then, malondialdehyde (MDA) levels and the superoxide dismutase (SOD) content were measured to evaluate the antioxidant effect of AEEF in the brain tissue. Treatment with AEEF showed a considerable ameliorating effect on biomarkers such as SOD and MDA content in alcohol-induced mice. To assess the cerebral cholinergic system involved in neuronal signaling, acetylcholinesterase (AChE) activity and acetylcholine (ACh) content were measured. The AEEF groups showed increased ACh levels and decreased AChE activities. In addition, AEEF prevented alcohol-induced neuronal apoptosis via improvement of mitochondrial activity, including reactive oxygen species levels, mitochondrial membrane potential, and adenosine triphosphate content. AEEF inhibited apoptotic signals by regulating phosphorylated c-Jun N-terminal kinases (p-JNK), phosphorylated protein kinase B (p-Akt), Bcl-2-associated X protein (BAX), and phosphorylated Tau (p-Tau). Finally, the bioactive compounds of AEEF were identified as caffeoylquinic acid (CQA), 3,5-dicaffeoylquinic acid (3,5-diCQA), and chikusetsusaponin IVa using the UPLC-Q-TOF-MS system.
Increased Frequency of Pfdhps A581G Mutation in Plasmodium falciparum Isolates from Gabonese HIV-Infected Individuals
Authors: Koumba Lengongo Jeanne Vanessa , Ndiaye Yaye Dié , Tshibola Mbuyi Marie Louise , Ndong Ngomo Jacques Mari , Ndiaye Daouda , Bouyou Akotet Marielle Karine , Mawili-Mboumba Denise Patricia
Published: May 2019

Background. Studying malaria parasites cross resistance to sulfadoxine-pyrimethamine (SP) and trimethoprim-sulfamethoxazole (cotrimoxazole, CTX) is necessary in areas coendemic for malaria and HIV. Polymorphism and frequency of drug resistance molecular markers, Pfdhfr and Pfdhps genes have been assessed in Plasmodium falciparum isolates from HIV-infected adults, in Gabon. Materiel and Methods. A cross-sectional study was conducted in three HIV care and treatment centers, at Libreville, the capital city of Gabon and at Oyem and Koulamoutou, two rural cities between March 2015 and June 2016. P. falciparum-infected HIV adults were selected. Analysis of Pfdhfr and Pfdhps genes was performed using high resolution melting (HRM) technique. Results. Pfdhps A581G mutation was found in 23.5% (8/34) of the isolates. Triple Pfdhfr mutation (51I-59R-108N) was predominant (29.4%; n=10) while 17.6% (n=6) of the isolates carried a quadruple mutation (Pfdhfr 51I-59R-108N + Pfdhps 437G; Pfdhfr 51I-108N + Pfdhps 437G-Pfdhps581G; Pfdhfr 51I-59R-108N + Pfdhps 581G). Highly resistant genotype was detected in around 10% (n=3) of the isolates. The quintuple mutation (triple Pfdhfr 51I-59R-108N and double Pfdhps437-581) was only found in isolates from two patients who did not use CTX. The most frequent haplotypes were those with a single mutation (NCNIAKA) (36%) and a quadruple mutation (NCIIGKG, NRIIGKA, and NRIIAKG). Mixed unknown genotypes were found at codon 164 in three isolates. Mixed genotypes were more frequent at codons 51 (23.5%; n=8) and 59 (20.5%; n=7) (p<0.01). Conclusion. Pfdhps A581G mutation as well as new combination of quintuple mutations is found for the first time in isolates from HIV-infected patients in Gabon in comparison to a previous study. The detection of these genotypes at a nonnegligible frequency underlines the need of a regular surveillance of antifolates drug resistance.
Research on the Radial Accuracy of Ultrasonic Vibration-Assisted Single Point Incremental Forming Parts
Authors: Mingshun Yang , Lang Bai , Yunbo Lin , Yan Li , Qilong Yuan , Renfeng Zhao
Published: May 2019

With the more and more complexity demands of the market, the geometric accuracy of the part has become the main factor restricting the development of single point incremental forming technology (SPIF). For this reason, with the truncated cone as the target part, the radial accuracy error generation mechanism was analyzed from the aspects of sheet springback and residual stress distribution. Four factors and three levels of surface response experiments were designed using the Box-Behnken Design (BBD) for tool head diameter, layer spacing, sheet thickness, and wall angle. The single and interactive influence law of the process parameters on the radial accuracy was obtained. In response to the above research results, the ultrasonic vibration was introduced into the process of SPIF to reduce springback by reducing residual stress. The influence of vibration parameters on the accuracy was obtained through experiments. The results showed that ultrasonic vibration could effectively improve and control the accuracy of the part.
Abstract A03: Risk prediction for gastric cancer using the GWAS-identified SNPs, Helicobacter pylori infection and lifestyle-related risk factors in a Japanese population
Authors: Ito Hidemi , Oze Isao , Matsuo Keitaro
Published: May 2019

Advances in molecular genetics have the potential to impact cancer prevention. However, the contribution of this information to determining the risk of cancer of the stomach at the population level in combination with biological and lifestyle-related factors has not been evaluated. Here, we established a risk prediction model of gastric cancer using genetic, biological, and lifestyle-related risk factors as a potential practical application in interventions for cancer prevention.

We conducted two independent age- and sex-matched case-control studies, the first for model derivation (697 cases and 1,372 controls) and the second (678 and 678) for external validation. Using the derivation study data, we developed a prediction model by fitting a conditional logistic regression model using the following predictors: age, ABCD classification defined by H.pylori infection and atrophic gastritis, smoking, fruit and vegetable intake, and selected GWAS-identified genotypes. Performance was assessed regarding discrimination (area under the curve, AUC), calibration (calibration plots and Hosmer-Lemeshow test) and reclassification (integrated discrimination improvement (IDI)).

We preliminarily found that a combination of rs229400, one of the GWAS-identified SNPs, H.Pylori infection, atrophic gastritis, smoking and fruit and vegetable intake provided high discriminatory accuracy and good calibration in both the derivation and validation studies, with AUCs of 0.78 (95% confidence intervals, 0.76-0.80) and 0.80 (0.77-0.82), respectively. The calibration plots of both studies stayed close to the ideal calibration line. IDI indices were 0.12 (p<0.00001). We will establish a final model with selected GWAS-identified SNPs in addition to rs2294008, and the other predictors.

Citation Format: Hidemi Ito, Isao Oze, Keitaro Matsuo. Risk prediction for gastric cancer using the GWAS-identified SNPs, Helicobacter pylori infection and lifestyle-related risk factors in a Japanese population. [abstract]. In: Proceedings of the AACR Special Conference: Improving Cancer Risk Prediction for Prevention and Early Detection; Nov 16-19, 2016; Orlando, FL. Philadelphia (PA): AACR; Cancer Epidemiol Biomarkers Prev 2017;26(5 Suppl):Abstract nr A03.