bluuebunny/crossref_metadata_2025 huggingface dataset:
Mesenchymal-Stem-Cell-Based Strategies for Retinal Diseases
Authors: Chen Xiteng , Jiang Yuanfeng , Duan Yanan , Zhang Xiaomin , Li Xiaorong
Published: October 2022

Retinal diseases are major causes of irreversible vision loss and blindness. Despite extensive research into their pathophysiology and etiology, pharmacotherapy effectiveness and surgical outcomes remain poor. Based largely on numerous preclinical studies, administration of mesenchymal stem cells (MSCs) as a therapeutic strategy for retinal diseases holds great promise, and various approaches have been applied to the therapies. However, hindered by the retinal barriers, the initial vision for the stem cell replacement strategy fails to achieve the anticipated effect and has now been questioned. Accumulating evidence now suggests that the paracrine effect may play a dominant role in MSC-based treatment, and MSC-derived extracellular vesicles emerge as a novel compelling alternative for cell-free therapy. This review summarizes the therapeutic potential and current strategies of this fascinating class of cells in retinal degeneration and other retinal dysfunctions.
Comparative Biochemical and Transcriptomic Analyses Provide New Insights into Phytoplasma Infection Responses in Cucumber
Authors: Wang Xueting , Hu Qiming , Wang Jiaxi , Lou Lina , Xu Xuewen , Chen Xuehao
Published: October 2022

Flat stem and witches’ broom phytoplasma-like symptoms in the cucumber inbred line C17 were observed in a greenhouse at Yangzhou University, China for three consecutive planting seasons; these symptoms resulted in a decreased yield. To better understand the cause of these symptoms, 16S rRNA PCR, plant hormones, mineral elements, and RNA-seq profiling were performed using symptomatic and normal stem samples. The results showed that the causal agent was classified as the Candidatus phytoplasma asteris strain, a plant pathogenic prokaryote that could not be cultured in vitro. Measurement of plant hormones showed that auxin, salicylic acid, and jasmonic acid contents were significantly increased, whereas that of ethylene’s immediate biosynthetic precursor, 1-aminocyclopropane-1-carboxylic acid, was decreased in the phytoplasma-infected stems compared with the healthy stems. Furthermore, measurement of mineral element composition showed that magnesium, calcium, sodium, iron, and zinc concentrations significantly changed in the phytoplasma-infected cucumber stems compared with the uninfected stems. Comparative RNA-seq identified 253 differentially expressed genes, including 179 upregulated and 74 downregulated genes. Further analyses suggested that genes related to phenylpropanoid biosynthesis, phenylalanine metabolism, and plant hormone signal transduction contributed to phytoplasma infection. Taken together, this study presents the first in-depth assessment of disease symptoms and biochemical content of cucumber stems known to be infected with phytoplasma.
Response of Care Services for Patients with HIV/AIDS during a Pandemic: Perspectives of Health Staff in Bolivia
Authors: López Liseth Lourdes Arias , Solis-Soto Maria Teresa
Published: October 2022

The COVID-19 pandemic has caused an unprecedented crisis striking health services, generating risks of setbacks in health care and affecting the most vulnerable populations such as HIV patients. This study aims to explore the impact of the COVID-19 pandemic on the operational management of health services for people living with HIV/AIDS in Cochabamba, Bolivia. We applied a qualitative approach using semi-structured in-depth interviews with ten key health professionals who care for patients with HIV/AIDS in Cochabamba, Bolivia. Interviews were transcribed verbatim and uploaded to Atlas.ti software for analysis. We used an ethnographic approach within the interpretive paradigm to carry out the thematic analysis, considering the “Determinants of health systems resilience framework” of five dimensions developed by the World Health Organization. Even though the provision of services in public care services was not interrupted during the COVID-19 pandemic, health service delivery was severely affected. Digital technology could be used to compensate in urban areas. Regarding the distribution of medications, adaptative strategies to reduce patient complications were implemented. Unfortunately, the complementary tests availability was limited. The COVID-19 pandemic had a significant impact on HIV/AIDS patient care services in Cochabamba, with repercussions for HIV treatment access and virologic suppression.
The Healing Environment of Dental Clinics through the Eyes of Patients and Healthcare Professionals: A Pilot Study
Authors: Sarapultseva Maria , Zolotareva Alena , Nasretdinova Natal’ya , Sarapultsev Alexey
Published: October 2022

The physical environment of healthcare settings can promote both the healing process and patient feelings of well-being, as well as instill positive emotions in employees. The present study aimed to evaluate the dental work environment of a typical private and public dental clinic to identify key parameters that determine the perception of health facilities by patients and employees. The study was carried out from 1 to 20 December 2021, in two dental clinics in Ekaterinburg (Russian Federation) using ‘ASPECT’. The participants were 58 staff and 94 patients. The results showed that, compared with patients, staff reported higher views scores, nature and outdoors scores, and comfort and control scores. The common criterion that distinguishes private clinics from public ones was comfort and control. Compared with patients in state clinics, patients in private clinics reported higher privacy, company and dignity scores, comfort and control scores, interior appearance scores, and facility scores. In general, while views scores and nature parameters can be singled out as a universal absolute value for everyone in a particular environment, staff pay more attention to factors that contribute to long-term comfortable stay and performance of their duties.
Canoe polo Athletes’ Anthropometric, Physical, Nutritional, and Functional Characteristics and Performance in a Rowing Task: Cross-Sectional Study
Authors: Marques-Sule Elena , Arnal-Gómez Anna , Monzani Lucas , Deka Pallav , López-Bueno Jairo P. , Saavedra-Hernández Manuel , Suso-Martí Luis , Espí-López Gemma V.
Published: October 2022

Understanding the physical, functional, mental, and nutritional attributes of canoe polo athletes is essential for training and development. Forty-three canoe polo athletes (mean age: 21.54 ± 6.03) participated in the study and were assessed for: anthropometric measurements, exercise motivation, eating habits, adherence to the Mediterranean Diet, and physical and functional abilities. Correlation and multivariate analysis were conducted. Individual performance in a rowing task showed body mass index (β = 0.41) and female gender (β = 0.34) to be the strongest anthropometric predictors, whereas body fat (β = −0.35) and triceps brachii skinfold fatty tissue (β = −0.35) were the strongest negative predictors. Pushing strength (β = 0.37) and range of motion with internal rotation (β = 0.30) were the strongest physical predictors. The physical dimension of the Exercise Motivation Index was a significant psychosocial predictor (β = 0.27). Senior participants had a higher waist–hip ratio (p = 0.04, d = 0.66), arm circumference (p = 0.03, d = 0.68), handgrip strength (p < 0.01, d = 1.27), and push strength (p < 0.01, d = 1.42) than under 21-year-olds. Understanding the highlighted sport-specific characteristics of canoe polo athletes can help trainers to design programs at all levels to optimize performance.
The Presence of Hyperhomocysteinemia Does Not Aggravate the Cardiometabolic Risk Imposed by Hyperuricemia in Young Individuals: A Retrospective Analysis of a Cross-Sectional Study
Authors: Šebeková Katarína , Gurecká Radana , Repiská Gabriela , Koborová Ivana , Podracká Ľudmila
Published: October 2022

Background: Little research has been conducted into the effects of the combined manifestation of hyperuricemia and hyperhomocysteinemia on cardiometabolic risk factors and markers in young subjects. Methods: 1298 males and 1402 females, 14-to-20-year-olds, were classified into four groups: 1/normouricemic/normohomocysteinemic, 2/normouricemic/hyperhormohomocysteinemic, 3/hyperuricemic/normohomocysteinemic, and 4/hyperuricemic/hyperhomocysteinemic. Anthropometric measures, blood pressure, plasma glucose, insulin, lipids, markers of renal function, C-reactive protein, asymmetric dimethylarginine, and blood counts were determined. Results: Hyperuricemic males (but not females) had higher odds for hyperhomocysteinemia than normouricemic ones (OR: 1.8; 95% CI: 1.4–2.3; p < 0.001). Homocysteine and uric acid levels correlated directly (males: r = 0.076, females: r = 0.120; p < 0.01, both). Two-factor analysis of variance did not reveal a significant impact of hyperhomocysteinemia on any of the investigated cardiometabolic variables in females; in males, hyperuricemia and hyperhomocysteinemia showed a synergic effect on asymmetric dimethylarginine levels. Among four groups, subjects concurrently manifesting hyperuricemia and hyperhomocysteinemia did not presented the highest continuous metabolic syndrome score—a proxy measure of cardiometabolic risk; neither the multivariate regression model indicated a concurrent significant effect of uric acid and homocysteine on continuous metabolic syndrome score in either sex. Conclusion: In young healthy subjects, hyperhomocysteinemia does not aggravate the negative health effects imposed by hyperuricemia.
Increasing Access to Care for the Underserved: Voices of Riders, Drivers, & Staff of a Rural Transportation Program
Authors: Schwartz Abby J. , Richman Alice R. , Scott Mallary , Liu Haiyong , White Weyling , Doherty Caroline
Published: October 2022

The qualitative data presented in this paper was part of a larger concurrent mixed methods study evaluating the effectiveness of a transportation program (Project TRIP) for low-income residents in rural eastern North Carolina. Twenty stakeholders involved in TRIP were interviewed, including riders (n = 12) of which 83% were over 50 years old, program staff including the program coordinator and 5 case managers (n = 6), and transportation providers (n = 2). Due to the COVID-19 pandemic, interviews were completed by phone with each participant. Themes from the qualitative data included the: (1) Emotional, health, & financial impacts of TRIP, (2) Changes that should be implemented into TRIP when replicating the program, and (3) Unique aspects of how TRIP operates that could inform other rural transportation programs. Thematic analysis was used to analyze the transcript data. The findings are couched in the context of how TRIP potentially defrays the impacts of cumulative disadvantage that residents experience over the life course by increasing access to healthcare.
Lockdown Amid COVID-19 Ascendancy over Ambient Particulate Matter Pollution Anomaly
Authors: Hassan Muhammad Azher , Mehmood Tariq , Lodhi Ehtisham , Bilal Muhammad , Dar Afzal Ahmed , Liu Junjie
Published: October 2022

Air is a diverse mixture of gaseous and suspended solid particles. Several new substances are being added to the air daily, polluting it and causing human health effects. Particulate matter (PM) is the primary health concern among these air toxins. The World Health Organization (WHO) addressed the fact that particulate pollution affects human health more severely than other air pollutants. The spread of air pollution and viruses, two of our millennium’s most serious concerns, have been linked closely. Coronavirus disease 2019 (COVID-19) can spread through the air, and PM could act as a host to spread the virus beyond those in close contact. Studies on COVID-19 cover diverse environmental segments and become complicated with time. As PM pollution is related to everyday life, an essential awareness regarding PM-impacted COVID-19 among the masses is required, which can help researchers understand the various features of ambient particulate pollution, particularly in the era of COVID-19. Given this, the present work provides an overview of the recent developments in COVID-19 research linked to ambient particulate studies. This review summarizes the effect of the lockdown on the characteristics of ambient particulate matter pollution, the transmission mechanism of COVID-19, and the combined health repercussions of PM pollution. In addition to a comprehensive evaluation of the implementation of the lockdown, its rationales—based on topographic and socioeconomic dynamics—are also discussed in detail. The current review is expected to encourage and motivate academics to concentrate on improving air quality management and COVID-19 control.
The Multidimensional Impacts of Inequities for Tāngata Whaikaha Māori (Indigenous Māori with Lived Experience of Disability) in Aotearoa, New Zealand
Authors: Ingham Tristram Richard , Jones Bernadette , Perry Meredith , King Paula Toko , Baker Gabrielle , Hickey Huhana , Pouwhare Rangi , Nikora Linda Waimarie
Published: October 2022

People with lived experience of disability have poorer health and socioeconomic outcomes than people without it. However, within this population, certain social groups are more likely to experience poorer outcomes due to the impacts of multiple intersecting forms of oppression including colonisation, coloniality and racism. This paper describes the multidimensional impacts of inequities for Indigenous tāngata whaikaha Māori (Māori with lived experience of disability). Semi-structured in-depth interviews were conducted with 28 tāngata whaikaha Māori and their whānau (extended family) using a kaupapa Māori Research methodology. An equity framework was used to analyse the data. The results describe: (1) inequitable access to the determinants of health and well-being; (2) inequitable access to and through health and disability care; (3) differential quality of health and disability care received; and (4) Indigenous Māori-driven solutions. These data confirm that tāngata whaikaha Māori in the nation-state known as New Zealand experience racism, ableism and disablism, compounded by the intersection between these types of discrimination. Recommendations from the data support the inclusion of tāngata whaikaha Māori in decision-making structures, including all policies and practices, along with equal partnership rights when it comes to designing health and disability systems and services.
Carfilzomib’s Real-World Safety Outcomes in Korea: Target Trial Emulation Study Using Electronic Health Records
Authors: Jang Ha Young , Lee Hyun Kyung , Kim Chae Jeong , Yoon Sung-Soo , Kim In-Wha , Oh Jung Mi
Published: October 2022

Carfilzomib is a promising anticancer drug for relapsed/refractory multiple myeloma (RRMM). However, real-world evidence has only investigated the cardiovascular safety of carfilzomib, and there is a high demand for thorough safety evaluations. We aimed to comprehensively evaluate the risk of adverse events associated with carfilzomib in Korean patients with RRMM. We followed up with 138 matched patients with RRMM (69 KRd (carfilzomib, lenalidomide, and dexamethasone) and 69 Rd (lenalidomide and dexamethasone) users). A total of 12 adverse events were evaluated. More than 75% of adverse events occurred during the early cycle (1–6 cycles), and the incidence rate showed a tendency to decrease in the later cycle (7–12 and 13–18 cycles). Severities of most adverse events were evaluated as grade 1-2. The KRd regimen were related with significantly increased risks of dyspnea (adjusted HR (aHR) 2.27, 95% confidence interval (CI) 1.24–4.16), muscle spasm (aHR 5.12, 95% CI 1.05–24.9) and thrombocytopenia (aHR 1.84, 95% CI 1.10–3.06). Although the severities were low, carfilzomib has many side effects in treating RRMM; hence, findings on the patterns of its adverse events could lead to both effective and safe use of KRd therapy in real-world settings.
Pricing Policies of Green Dual-Channel Supply Chain with Fairness Concerns and Altruistic Preferences Based on Consumers’ Environmental Awareness and Channel Preference
Authors: Ge Genhasi , Wang Daoping , Epede Mesumbe Bianca
Published: October 2022

Taking into consideration fairness concerns and altruistic preferences of manufacturers, this paper aims to propose a green dual-channel supply chain that incorporates consumers’ environmental awareness (CEA) and channel preference. The purpose of this work is to explore and further compare the optimal outcomes in a green dual-channel supply chain in three scenarios, which are the fairness-neutrality scenario (Model N), the manufacturer is concerned with fairness scenario (Model F), and the manufacturer has altruistic preference (Model A), respectively. The game-theoretical models with different fairness preferences, comparative, and numerical analyses are used to put forward the impacts of consumers’ channel preference and CEA on pricing, profits, and utilities, and to identify the differences in decisional outcomes between the three models. The results indicate that CEA always contributes to developing the green market while adversely affecting common products. Moreover, consumers’ channel preferences might enable the manufacturer and retailer to enhance profitability under certain conditions. The findings also reveal that manufacturer’s fairness concerns can possibly increase the demand for green products but impair the overall performance of the supply chain in general. Moreover, while the manufacturer’s altruistic preference benefits the retailer’s profits, it has a detrimental effect on the performance of the green supply chain. The practical implications of this research come to promote green consumption and increasing consumer awareness of environmental protection are effective ways to develop a green supply chain. It is also important to note that in order to maintain the durability and stability of the sup-ply chain, the manufacturer must maintain a moderate level of fairness preference behaviors so that downstream retailers will remain enthusiastic about establishing long-term relationships.
The Polygonal 3D Layout Reconstruction of an Indoor Environment via Voxel-Based Room Segmentation and Space Partition
Authors: Yang Fan , Li You , Che Mingliang , Wang Shihua , Wang Yingli , Zhang Jiyi , Cao Xinliang , Zhang Chi
Published: October 2022

An increasing number of applications require the accurate 3D layout reconstruction of indoor environments. Various devices including laser scanners and color and depth (RGB-D) cameras can be used for this purpose and provide abundant and highly precise data sources. However, due to indoor environment complexity, existing noise and occlusions caused by clutter in acquired data, current studies often require the idealization of the architecture space or add an implication hypothesis to input data as priors, which limits the use of these methods for general purposes. In this study, we propose a general 3D layout reconstruction method for indoor environments. The method combines voxel-based room segmentation and space partition to build optimum polygonal models. It releases idealization of the architectural space into a non-Manhattan world and can accommodate various types of input data sources, including both point clouds and meshes. A total of four point cloud datasets, four mesh datasets and two cross-floor datasets were used in experiments. The results exhibit more than 80% completeness and correctness as well as high accuracy.
The Molecular Mechanisms of Liver Fibrosis and Its Potential Therapy in Application
Authors: Zhang Danyan , Zhang Yaguang , Sun Bing
Published: October 2022

Liver fibrosis results from repeated and persistent liver damage. It can start with hepatocyte injury and advance to inflammation, which recruits and activates additional liver immune cells, leading to the activation of the hepatic stellate cells (HSCs). It is the primary source of myofibroblasts (MFs), which result in collagen synthesis and extracellular matrix protein accumulation. Although there is no FDA and EMA-approved anti-fibrotic drug, antiviral therapy has made remarkable progress in preventing or even reversing the progression of liver fibrosis, but such a strategy remains elusive for patients with viral, alcoholic or nonalcoholic steatosis, genetic or autoimmune liver disease. Due to the complexity of the etiology, combination treatments affecting two or more targets are likely to be required. Here, we review the pathogenic mechanisms of liver fibrosis and signaling pathways involved, as well as various molecular targets for liver fibrosis treatment. The development of efficient drug delivery systems that target different cells in liver fibrosis therapy is also summarized. We highlight promising anti-fibrotic events in clinical trial and preclinical testing, which include small molecules and natural compounds. Last, we discuss the challenges and opportunities in developing anti-fibrotic therapies.
Attachment Performance of Stick Insects (Phasmatodea) on Plant Leaves with Different Surface Characteristics
Authors: Burack Judith , Gorb Stanislav N. , Büscher Thies H.
Published: October 2022

Herbivorous insects and plants exemplify a longstanding antagonistic coevolution, resulting in the development of a variety of adaptations on both sides. Some plant surfaces evolved features that negatively influence the performance of the attachment systems of insects, which adapted accordingly as a response. Stick insects (Phasmatodea) have a well-adapted attachment system with paired claws, pretarsal arolium and tarsal euplantulae. We measured the attachment ability of Medauroidea extradentata with smooth surface on the euplantulae and Sungaya inexpectata with nubby microstructures of the euplantulae on different plant substrates, and their pull-off and traction forces were determined. These species represent the two most common euplantulae microstructures, which are also the main difference between their respective attachment systems. The measurements were performed on selected plant leaves with different properties (smooth, trichome-covered, hydrophilic and covered with crystalline waxes) representing different types among the high diversity of plant surfaces. Wax-crystal-covered substrates with fine roughness revealed the lowest, whereas strongly structured substrates showed the highest attachment ability of the Phasmatodea species studied. Removal of the claws caused lower attachment due to loss of mechanical interlocking. Interestingly, the two species showed significant differences without claws on wax-crystal-covered leaves, where the individuals with nubby euplantulae revealed stronger attachment. Long-lasting effects of the leaves on the attachment ability were briefly investigated, but not confirmed.
Size Measurement and Segmentectomy Resection Margin of Early-Stage Lung Adenocarcinoma Manifesting on Virtual 3D Imagery and Pathology: A Pilot Correlation Study
Authors: Lin Ching-Min , Tai Hui-Chun , Cheng Ya-Fu , Ke Pei-Cing , Liu Chia-Chi , Wang Bing-Yen
Published: October 2022

Background: The objective of our study was to assess if 3D reconstructed images could be extrapolated to reflect pathologies, as evaluated by early-stage lung adenocarcinoma tumor size and simulated segmentectomy resection margin. Methods: Retrospectively selected patients (n = 18) who underwent segmentectomy at Changhua Christian Hospital between 2012 and 2018 and then had pulmonary 3D reconstruction using Ziostation2 were included in our study. Tumor size and simulated segmentectomy resection distance on a 3D model were measure and compared to pathology. Results: Both tumor size and segmentectomy resection margin showed positive correlations between 3D image measurements and pathological measurements. The resection margin showed a stronger correlation and was beneficial in pre-operative planning. Conclusions: A 3D reconstructed model aided understanding of pulmonary anatomy, prompting confidence in surgical approaches and ensured segmentectomy outcome success. Regardless of age and pulmonary function, 3D simulation can accurately mimic segmentectomy, making it a simple, effective and feasible pre-operative planning tool.
Efficacy of Neurostimulations for Upper Extremity Function Recovery after Stroke: A Systematic Review and Network Meta-Analysis
Authors: Xue Tao , Yan Zeya , Meng Jiahao , Wang Wei , Chen Shujun , Wu Xin , Gu Feng , Tao Xinyu , Wu Wenxue , Chen Zhouqing , Bai Yutong , Wang Zhong , Zhang Jianguo
Published: October 2022

Background: Neurostimulations for the post-stroke recovery of upper extremity function has been explored in previous research, but there remains a controversy about the superiority of different neurostimulations. Methods: Randomized controlled trials (RCTs) were searched in MEDLINE, Embase, Cochrane Library and ClinicalTrials.gov, from 1 January 2000 to 1 June 2022. A conventional pair-wise meta-analysis with a random-effect model was used to evaluate direct evidence. Bayesian random effect models were used for network meta-analysis. The grading of the recommendations assessment, development and evaluation (GRADE) approach was applied to assess the clinical quality of the results. Results: A total of 88 RCTs, which enrolled 3491 participants, were included. For the Fugl-Meyer Assessment-Upper Extremity score change from the baseline to the longest follow-up, the following interventions showed a significant difference: VNS (MD = 4.12, 95%CrI: 0.54 to 7.80, moderate certainty), cNMES (MD = 3.98, 95%CrI: 1.05 to 6.92, low certainty), FES (MD = 7.83, 95%CrI: 4.42 to 11.32, very low certainty), drTMS (MD = 7.94, 95%CrI: 3.71 to 12.07, moderate certainty), LFrTMS (MD = 2.64, 95%CrI: 1.20 to 4.11, moderate certainty), HFrTMS (MD = 6.73, 95%CrI: 3.26 to 10.22, moderate certainty), and iTBS combined with LFrTMS (MD = 5.41, 95%CrI: 0.48 to 10.35, moderate certainty). Conclusions: The neurostimulations above the revealed significant efficacy for improving the upper limb function after stroke eased the suffering of the patient.
Reproductive Lifespan and Motor Progression of Parkinson’s Disease
Authors: Ou Ruwei , Wei Qianqian , Hou Yanbing , Zhang Lingyu , Liu Kuncheng , Lin Junyu , Yang Tianmi , Yang Jing , Jiang Zheng , Song Wei , Cao Bei , Shang Huifang
Published: October 2022

Objectives: Estrogen not only plays a key role in the decreased risk of Parkinson’s disease (PD) but also influences its severity. We aimed to explore the effect of the reproductive lifespan on the motor progression of PD female patients in a large prospective cohort study. Methods: A competing risk analysis with a Fine and Gray model on 491 female and 609 male patients with PD was conducted. We regarded the chance of faster motor progression (as measured by the Unified Parkinson’s Disease Rating Scale (UPDRS) III increasing by ≥16 points during follow-up) and the chance of death as competing risks. The reproductive lifespan was regarded as the variable of interest, while faster motor progression was set as the primary outcome. Results: In the multivariable competing risk analysis, the male sex was not significantly associated with faster motor progression (subdistribution hazard ratio (SHR) 0.888, 95% CI 0.652–1.209, p = 0.450), while a shorter reproductive lifespan was associated with faster motor progression in women (SHR 0.964, 95% CI 0.936–0.994, p = 0.019). Sensitivity analysis indicated that a shorter reproductive lifespan was also significantly associated with faster motor progression in the 48 female patients who reported menopause after the onset of PD (SHR 0.156, 95% CI 0.045–0.542, p = 0.003). A linear mixed model also revealed the significant main effects of a short reproductive lifespan on the higher UPDRS III score in PD female patients at the last visit (p = 0.026). Conclusions: Our study indicates that a short reproductive lifespan contributes to faster motor progression in PD female patients, which has important implications for understanding the role of endogenous estrogen exposure in female PD and is beneficial to select appropriate patients in clinical trials.
Comparison of Morphological and Digital-Assisted Analysis for BCL6 Endometrial Expression in Women with Endometriosis
Authors: Squatrito Marlyne , Blacher Silvia , Henry Laurie , Labied Soraya , Noel Agnès , Nisolle Michelle , Munaut Carine
Published: October 2022

BCL6 (B-cell lymphoma 6) is a proto-oncogene and transcriptional repressor initially described as being involved in B-cell lymphoma. Recently, this factor has been identified as a promising tissue biomarker which could be used to diagnose women affected by endometriosis. Previous studies used HSCORE for BCL6 staining quantification in the endometrium. However, this semi-quantitative technique of analysis has some limitations, including a lack of objectivity, robustness, and reproducibility that may lead to intra- and inter-observer variability. Our main goal was to develop an original computer-assisted method to quantify BCL6 staining from whole-slide images reliably. In order to test the efficiency of our new digital method of quantification, we compared endometrial BCL6 expression between fertile and infertile women without or with different stages of endometriosis by using the widely used HSCORE analysis and our new automatic digital image analysis. We find a higher expression of BCL6 in the endometrium of infertile women with endometriosis and women with stage IV endometriosis. Furthermore, we demonstrate a significant correlation between the two types of independent measurements, indicating the robustness of results and also the reliability of our computer-assisted method for BCL6 quantification. In conclusion, our work, by using this original computer-assisted method, enables BCL6 quantification more objectively, reliably, robustly, and promptly compared to HSCORE analysis.
Associations of the Alpha-Actinin Three Genotype with Bone and Muscle Mass Loss among Middle-Aged and Older Adults
Authors: Taniguchi Yoshiaki , Makizako Hyuma , Nakai Yuki , Kiuchi Yuto , Akaida Shoma , Tateishi Mana , Takenaka Toshihiko , Kubozono Takuro , Ohishi Mitsuru
Published: October 2022

Bone and muscle mass loss are known to occur simultaneously. The alpha-actinin three (ACTN3) genotype has been shown to potentially affect bone and muscle mass. In this study, we investigated the association between the ACTN3 genotype and bone and muscle mass loss in community-dwelling adults aged ≥ 60 years. This study was a cross-sectional analysis of data from 295 participants who participated in a community health checkup. The ACTN3 genotypes were classified as RR, RX, or XX types. Bone mass loss was defined as a calcaneal speed of sound T-score of <−1.32 and <−1.37, and muscle mass loss was defined as an appendicular skeletal muscle index of <7.0 kg/m2 and <5.7 kg/m2 in men and women, respectively. The percentages of XX, RX, and RR in the combined bone and muscle mass loss group were 33.8%, 30.8%, and 16.7%, respectively, with a significantly higher trend for XX. Multinomial logistic regression analysis showed that XX had an odds ratio of 3.00 (95% confidence interval 1.05–8.54) of being in the combined bone and muscle mass loss group compared to the RR group (covariates: age, sex, grip strength, and medications). The ACTN3 genotype of XX is associated with a higher rate of comorbid bone and muscle mass loss. Therefore, ACTN3 genotyping should be considered for preventing combined bone and muscle mass loss.
A Review of Point Absorber Wave Energy Converters
Authors: Guo Bingyong , Wang Tianyao , Jin Siya , Duan Shunli , Yang Kunde , Zhao Yaming
Published: October 2022

There are more than thousands of concepts for harvesting wave energy, and wave energy converters (WECs) are diverse in operating principles, design geometries and deployment manners, leading to misconvergence in WEC technologies. Among numerous WEC devices, the point absorber wave energy converter (PAWEC) concept is one of the simplest, most broad-based and most promising concepts that has been investigated intensively all over the world. However, there are only a few reviews focusing on PAWECs, and the dynamical advancement of PAWECs merits an up-to-date review. This review aims to provide a critical overview of the state of the art in PAWEC development, comparing and contrasting various PAWEC devices and discussing recent research and development efforts and perspectives of PAWECs in terms of prototyping, hydrodynamic modelling, power take-off mechanism and control.
Optimization of Process Parameters in Friction Stir Welding of Aluminum 5451 in Marine Applications
Authors: Ahmed Shoaib , Rahman Rana Atta ur , Awan Awais , Ahmad Sajjad , Akram Waseem , Amjad Muhammad , Yahya Mohd Yazid , Rahimian Koloor Seyed Saeid
Published: October 2022

Friction stir welding (FSW) is one of the primary fabrication techniques for joining different components, and it has become popular, especially in aluminum alloy structures for marine applications. The welded joint with the friction stir process greatly depends on the process parameters, i.e., feed rate, rotational speed, and pin profile of the tool. In the current study, plates of aluminum 5451 alloy were joined by the FSW technique, and the Taguchi method was used to find the process parameters at an optimal level. The maximum value of tensile strength, i.e., 160.6907 MPa, was achieved using optimum welding conditions of a tool rotation speed of 1400, a feed rate of 18 mm/min, and the tool pin with threads. The maximum value of hardness, i.e., 81.056 HV, was achieved using optimum conditions of 1200 tool rotational speed and a feed rate of 18 mm/min with a tool pin profile having threads. In addition, the contribution in terms of the percentage of each input parameter was found by the analysis of variance (ANOVA). The ANOVA results revealed that the pin profile of the tool has the maximum contribution of 67.77% and 62.42% in achieving the optimum value of tensile strength and hardness, respectively. The study also investigated the joint efficiency of the friction stir welded joint, hardness at the weld zone, and metallography on FSW samples at the optimized level. The effectiveness and reliability of FSW joints for shipping industry applications can be observed by joint efficiency. That was investigated at optimum conditions, and it comes out to be 80.5%.
Relatives from Hereditary Breast and Ovarian Cancer and Lynch Syndrome Families Forgoing Genetic Testing: Findings from the Swiss CASCADE Cohort
Authors: Sarki Mahesh , Ming Chang , Aceti Monica , Fink Günther , Aissaoui Souria , Bürki Nicole , Graffeo Rossella , Heinimann Karl , Caiata Zufferey Maria , Monnerat Christian , Rabaglio Manuela , Zürrer-Härdi Ursina , Chappuis Pierre O. , Katapodi Maria C. ,   the CASCADE Consortium
Published: October 2022

Cascade genetic testing of relatives from families with pathogenic variants associated with hereditary breast and ovarian cancer (HBOC) or Lynch syndrome (LS) has important implications for cancer prevention. We compared the characteristics of relatives from HBOC or LS families who did not have genetic testing (GT (−) group) with those who had genetic testing (GT (+) group), regardless of the outcome. Self-administered surveys collected cross-sectional data between September 2017 and December 2021 from relatives participating in the CASCADE cohort. We used multivariable logistic regression with LASSO variable selection. Among n = 115 relatives who completed the baseline survey, 38% (n = 44) were in the GT (−) group. Being male (OR: 2.79, 95% CI: 1.10–7.10) and without a previous cancer diagnosis (OR: 4.47, 95% CI: 1.03–19.42) increased the odds of being untested by almost three times. Individuals from families with fewer tested relatives had 29% higher odds of being untested (OR: 0.71, 95% CI: 0.55–0.92). Reasons for forgoing cascade testing were: lack of provider recommendation, lack of time and interest in testing, being afraid of discrimination, and high out-of-pocket costs. Multilevel interventions designed to increase awareness about clinical implications of HBOC and LS in males, referrals from non-specialists, and support for testing multiple family members could improve the uptake of cascade testing.
Measuring Using Disruptive Technology in the Supply Chain Context: Scale Development and Validation
Authors: Özkanlısoy Özden , Bulutlar Füsun
Published: October 2022

The concept of disruptive technology has been in our lives for many years, and it is essential to measure their utilization levels to survive in the global competitive environment, to benefit from their contributions to supply chains, to examine their relations with supply chain operations and to compare them with average state of the industry. The aim of this study is to develop and validate a measurement instrument for supply chain management practices in the disruptive technology field. Accordingly, the study was carried out in five steps and the sample size consists of 47 companies as pilot data and 426 companies for the main data. These steps consist of item generation and purification, pilot test, initial identification of dimensionality, dimensionality confirmation and convergent validity assessment. As a result of the study, a new scale with a single factor structure was developed. The study ends with the evaluation of the findings. Correcting the lack of a measurement tool developed in this field in the literature is the theoretical contribution of the study. Furthermore, this study enables supply chain leaders to compare their utilization level of disruptive technology with the industries in which they operate, to associate it with operations and to enhance technology investments in practice.
Comparative Evaluation of Mountain Landscapes in Beijing Based on Social Media Data
Authors: Ding Tingting , Sun Wenzhuo , Wang Yuan , Yu Rui , Ge Xiaoyu
Published: October 2022

An important part of Beijing’s ecological pattern, mountain landscapes are also the most important natural tourist destinations in Beijing. The unique mountain environment in Taihang and Yan Mountains attracts Beijing and foreign tourists alike. Tourists publish travel photos and comments on social media, which provides a new opportunity for a systematic evaluation of these mountain parks based on social media data. To fully understand the developmental status of mountain landscapes in Beijing, this paper comparatively evaluates 45 mountain landscapes in Beijing based on social media data. Using big data capture, semantic network analysis, importance-performance analysis (IPA), etc., it explores the composition of tourist groups in mountain parks, the preferences of the tourist groups, and the relationships between park tourists and different influencing factors, and evaluates the recreational experiences of tourist groups. The development of recreational activities was found to be more important to local tourists than scenic sites for foreign tourists. According to gender differences, women were more interested in recreational experiences than men, while men were more interested in the park’s landscapes. According to the IPA, tourists were satisfied with the overall recreation offered by mountain landscapes. The perceptual experience was dominated by visual perception, followed by smell; touch, hearing, and taste were of minor importance. Using social media data to analyze mountain landscape resources in Beijing can provide useful insights into the advantages of these landscapes under a variety of site conditions, strengthen local mountain resource development and tourism publicity, integrate tourism management and planning resources in a targeted and attractive manner, and enhance ecological leisure services.
Impact of Roads on Environmental Protected Areas: Analysis and Comparison of Metrics for Assessing Habitat Fragmentation
Authors: Sánchez-Fernández Manuel , Barrigón Morillas Juan Miguel , Montes González David , de Sanjosé Blasco José Juan
Published: October 2022

The present study focuses on evaluating the effect of fragmentation caused by road infrastructures on a territory with singular characteristics such as low population density and a high proportion of its surface area protected by the Natura 2000 network. Based on the IFI, UFI, Meff and DIVI metrics, the state of fragmentation of the landscape units (LU) was studied from two different approaches, considering two different protection figures, and the degree of suitability of the metrics used for the objective pursued was analysed. The results show that the expressions proposed for the indicators which measure the fragmentation of landscape units (LU) originated by road infrastructures (IFI, Meff and DIVI) assess different causes and consequences in the territory than that proposed for fragmentation originated by urban areas (UFI). The combination of all indicators allows for the identification of shortcomings and strengths of the LU analysed and, consequently, evaluation of the effectiveness of the design of the LU and need for improvement. The outcomes of fragmentation analysis of the LU in the area under study varied depending on the criterion applied and the protection figure considered. A general increasing trend for all indicators was found in terms of the number of LU units and LU surface as the level of fragmentation rises. The results of this study are useful for decision-making on territory and road infrastructures management and new approaches to the organisation of the Natura 2000 network.
Comprehensive Approaches in the Global Compact for Migration and the EU Border Policies: A Critical Appraisal
Authors: Vitiello Daniela
Published: October 2022

The quest for safe, orderly and regular migration underpins the UN Global Compact for Migration (GCM) and translates into “comprehensive and integrated” responses to large movements of refugees and migrants. The effort to de-compartmentalise the governance of cross-border human mobility through “comprehensiveness” shapes the overall search for greater policy coherence via regime interaction and shared responsibility within the GCM. A similar effort has been made at the EU level to overcome the “silos approach” characterising the distinct policies on migration, asylum, and border management. This parallelism is particularly meaningful as the reason is twofold: at the operational level, because of the role played by the EU in fashioning the cooperation models underpinning the GCM, which enhances the relevance of EU law and practice for the implementation of the GCM; at the normative level, because the GCM draws on four guiding principles—i.e., sovereignty, good governance, human-centricity, and the rule of law—which are also key features of the EU legal system. Departing from these premises, this article reveals the meaning of “comprehensive and integrated” responses to large movements of refugees and migrants in the GCM and EU border policies. It does so in order to provide a critical appraisal of the legal and policy implications of comprehensive approaches in the global and European governance of cross-border human mobility.
Evolution of Proliferative Model Protocells Highly Responsive to the Environment
Authors: Matsuo Muneyuki , Toyota Taro , Suzuki Kentaro , Sugawara Tadashi
Published: October 2022

In this review, we discuss various methods of reproducing life dynamics using a constructive approach. An increase in the structural complexity of a model protocell is accompanied by an increase in the stage of reproduction of a compartment (giant vesicle; GV) from simple reproduction to linked reproduction with the replication of information molecules (DNA), and eventually to recursive proliferation of a model protocell. An encounter between a plural protic catalyst (C) and DNA within a GV membrane containing a plural cationic lipid (V) spontaneously forms a supramolecular catalyst (C@DNA) that catalyzes the production of cationic membrane lipid V. The local formation of V causes budding deformation of the GV and equivolume divisions. The length of the DNA strand influences the frequency of proliferation, associated with the emergence of a primitive information flow that induces phenotypic plasticity in response to environmental conditions. A predominant protocell appears from the competitive proliferation of protocells containing DNA with different strand lengths, leading to an evolvable model protocell. Recently, peptides of amino acid thioesters have been used to construct peptide droplets through liquid–liquid phase separation. These droplets grew, owing to the supply of nutrients, and were divided repeatedly under a physical stimulus. This proposed chemical system demonstrates a new perspective of the origins of membraneless protocells, i.e., the “droplet world” hypothesis. Proliferative model protocells can be regarded as autonomous supramolecular machines. This concept of this review may open new horizons of “evolution” for intelligent supramolecular machines and robotics.
Left Ventricular Remodeling and Heart Failure Predictors in Acute Myocardial Infarction Patients with Preserved Left Ventricular Ejection Fraction after Successful Percutaneous Intervention in Western Romania
Authors: Arnautu Diana-Aurora , Andor Minodora , Buz Bogdan-Flaviu , Tomescu Mirela-Cleopatra , Vacarescu Cristina , Crisan Simina , Gaita Dan , Luca Constantin-Tudor , Cozma Dragos
Published: October 2022

(1) Acute myocardial infarction (AMI) patients are at risk of left ventricular (LV) remodeling and heart failure (HF), even after successful revascularization by percutaneous coronary intervention (PCI). We wanted to assess the independent predictors of these outcomes in AMI patients. (2) Methods: The study enrolled patients with a LVEF ≥50% after a successful PCI for their first AMI. After 24 months, patients were separated into two groups based on whether their LVEF remained ≥50% (group I), or decreased to <50% (group II). (3) Outcomes: 26% of the patients experienced a decrease in LVEF below 50%, 41% showed LV remodeling, and 8% had experienced HF hospitalizations. HF hospitalizations were significantly more frequent in group II patients (p < 0.0001). The Killip class at admission >2, infarct-related longitudinal strain ≤−12.5%, and the presence of LV remodeling were identified as independent predictors of HF hospitalizations. (4) Conclusions: About 26% of AMI patients with normal LV function after a successful PCI developed HF. More sensitive techniques are required that allow for a more efficient risk-stratification and preventive therapy to reduce LV remodeling and HF in AMI patients with LVEF ≥50% after a successful PCI. The detection of abnormal ventricular deformation patterns after PCI by speckle-tracking echocardiography might be a valuable method in this approach.
Green Synthesis and Characterization of Silver Nanoparticles Using a Lythrum salicaria Extract and In Vitro Exploration of Their Biological Activities
Authors: Corciovă Andreia , Mircea Cornelia , Burlec Ana Flavia , Fifere Adrian , Moleavin Ioana Turin , Sarghi Alexandra , Tuchiluș Cristina , Ivănescu Bianca , Macovei Irina
Published: October 2022

This research describes an eco-friendly green route for the synthesis of AgNPs using an aqueous extract of Lythrum salicaria. Taguchi design was used to optimize the synthesis method, taking into account various working conditions. The optimum parameters were established using a 3 mM AgNO3 concentration, a 1:9 extract:AgNO3 volume ratio, a pH value of 8, 60 ℃ temperature, and 180 min reaction time. The synthesized AgNPs were characterized using UV-Vis and FTIR spectroscopy, and TEM and EDX analysis. The SPR band at 410 nm, as well as the functional groups of biomolecules identified by FTIR and the EDX signals at ~3 keV, confirmed the synthesis of spherical AgNPs. The average AgNPs size was determined to be 40 nm, through TEM, and the zeta potential was −19.62 mV. The antimicrobial assay showed inhibition against S. aureus and C. albicans. Moreover, the results regarding the inhibition of lipoxygenase and of peroxyl radical-mediated hemolysis assays were promising and justify further antioxidant studies.