bluuebunny/crossref_metadata_2025 huggingface dataset:
Evaluating the Effects of an Organic Extract from the Mediterranean Sponge Geodia cydonium on Human Breast Cancer Cell Lines
Authors: Costantini Susan , Guerriero Eliana , Teta Roberta , Capone Francesca , Caso Alessia , Sorice Angela , Romano Giovanna , Ianora Adrianna , Ruocco Nadia , Budillon Alfredo , Costantino Valeria , Costantini Maria
Published: October 2017

Marine sponges are an excellent source of bioactive secondary metabolites for pharmacological applications. In the present study, we evaluated the chemistry, cytotoxicity and metabolomics of an organic extract from the Mediterranean marine sponge Geodia cydonium, collected in coastal waters of the Gulf of Naples. We identified an active fraction able to block proliferation of breast cancer cell lines MCF-7, MDA-MB231, and MDA-MB468 and to induce cellular apoptosis, whereas it was inactive on normal breast cells (MCF-10A). Metabolomic studies showed that this active fraction was able to interfere with amino acid metabolism, as well as to modulate glycolysis and glycosphingolipid metabolic pathways. In addition, the evaluation of the cytokinome profile on the polar fractions of three treated breast cancer cell lines (compared to untreated cells) demonstrated that this fraction induced a slight anti-inflammatory effect. Finally, the chemical entities present in this fraction were analyzed by liquid chromatography high resolution mass spectrometry combined with molecular networking.
Addendum: Palmieri, O. et al. Functional Implications of MicroRNAs in Crohn’s Disease Revealed by Integrating MicroRNA and Messenger RNA Expression Profiling. Int. J. Mol. Sci. 2017, 18, 1580
Authors: Palmieri Orazio , Creanza Teresa , Bossa Fabrizio , Latiano Tiziana , Corritore Giuseppe , Palumbo Orazio , Martino Giuseppina , Biscaglia Giuseppe , Scimeca Daniela , Carella Massimo , Ancona Nicola , Andriulli Angelo , Latiano Anna
Published: October 2017

The authors would like to indicate that Dr. Orazio Palmieri and Dr. Teresa Maria Creanza contributed equally to the work published in the International Journal of Molecular Sciences [1] [...]
A Central Bioactive Region of LTBP-2 Stimulates the Expression of TGF-β1 in Fibroblasts via Akt and p38 Signalling Pathways
Authors: Sideek Mohamed , Smith Joshua , Menz Clementine , Adams Julian , Cowin Allison , Gibson Mark
Published: October 2017

Latent transforming growth factor-β-1 binding protein-2 (LTBP-2) belongs to the LTBP-fibrillin superfamily of extracellular proteins. Unlike other LTBPs, LTBP-2 does not covalently bind transforming growth factor-β1 (TGF-β1) but appears to be implicated in the regulation of TGF-β1 bioactivity, although the mechanisms are largely unknown. In experiments originally designed to study the displacement of latent TGF-β1 complexes from matrix storage, we found that the addition of exogenous LTBP-2 to cultured human MSU-1.1 fibroblasts caused an increase in TGF-β1 levels in the medium. However, the TGF-β1 increase was due to an upregulation of TGF-β1 expression and secretion rather than a displacement of matrix-stored TGF-β1. The secreted TGF-β1 was mainly in an inactive form, and its concentration peaked around 15 h after addition of LTBP-2. Using a series of recombinant LTBP-2 fragments, the bioactivity was identified to a small region of LTBP-2 consisting of an 8-Cys motif flanked by four epidermal growth factor (EGF)-like repeats. The LTBP-2 stimulation of TGF-β expression involved the phosphorylation of both Akt and p38 mitogen-activated protein kinase (MAPK) signalling proteins, and specific inactivation of each protein individually blocked TGF-β1 increase. The search for the cell surface receptor mediating this LTBP-2 activity proved inconclusive. Inhibitory antibodies to integrins β1 and αVβ5 showed no reduction of LTBP-2 stimulation of TGF-β1. However, TGF-β1 upregulation was partially inhibited by anti-αVβ3 integrin antibodies, suggestive of a direct or indirect role for this integrin. Overall, the study indicates that LTBP-2 can directly upregulate cellular TGF-β1 expression and secretion by interaction with cells via a short central bioactive region. This may be significant in connective tissue disorders involving aberrant TGF-β1 signalling.
Newborn Screening for Primary T- and B-Cell Immune Deficiencies—A Prospective Study in Andalucía
Authors: De Felipe Beatriz , Olbrich Peter , Goycochea-Valdivia Walter , Delgado-Pecellin Carmen , Sanchez-Moreno Paula , Sánchez Berta , Lucena José , Ferrari-Cortes Araceli , De Soto Joséfa , Marquez Josefina , Salamanca Carmen , Jimenez Contreras Carlos , Neth Olaf
Published: October 2017

Background: Quantification of T-cell-receptor-excision circles (TRECs) and kappa-deleting-recombination-excision circles (KRECs) from dried blood spots (DBS) allows detection of neonates with severe T-cell and/or B-cell lymphopenia that are potentially affected by severe combined immunodeficiency (SCID), as well as X-linked agammaglobulinemia (XLA). Methods: Determination of TRECs and KRECs using a triplex RT-PCR (TRECS-KRECS-β-actin) assay from prospectively collected DBS between February 2014 and December 2016 in three hospitals in Seville, Spain. Cut-off levels were TRECs < 6/punch, KRECs < 4/punch and b-actin > 700/punch. Internal (SCID, XLA, ataxia telangiectasia) and external controls (CDC) were included. Results: A total of 8943 DBS samples obtained from 8814 neonates were analysed. Re-punching was necessary in 124 samples (1.4%) due to insufficient β-actin values (<700 copies/punch). Preterm neonates (GA < 37 weeks) and neonates with a BW < 2500 g showed significantly lower TRECs and KRECs levels (p < 0.001). Due to repeated pathological results, ten neonates were re-sampled (0.11%), of which five neonates (0.055%) confirmed the pathological results: one case was a fatal chromosomopathy (TRECs 1/KRECs 4); two were extreme premature newborns (TRECs 0/KRECs 0 and TRECs 1/KRECs 20 copies/punch); and 2 neonates were born to mothers receiving azathioprine during pregnancy (TRECs 92/KRECs 1 and TRECs 154/KRECs 3 copies/punch). All controls were correctly identified. Conclusions: Severe T- and B-cell lymphopenias were correctly identified by the TRECS-KRECS-β-actin assay. Prematurity and low BW are associated with lower TREC and KREC levels. Extreme prematurity and maternal immune suppressive therapy can cause false positive results of TRECs and KRECs values.
Multi-Path Data Distribution Mechanism Based on RPL for Energy Consumption and Time Delay
Authors: Zhu Licai , Wang Ruchuan , Yang Hao
Published: October 2017

The RPL (Routing Protocol for LLN) protocol is a routing protocol for low power and lossy networks. In such a network, energy is a very scarce resource, so many studies are focused on minimizing global energy consumption. End-to-end latency is another important performance indicator of the network, but existing research tends to focus more on energy consumption and ignore the end-to-end delay of data transmission. In this paper, we propose a kind of energy equalization routing protocol to maximize the surviving time of the restricted nodes so that the energy consumed by each node is close to each other. At the same time, a multi-path forwarding route is proposed based on the cache utilization. The data is sent to the sink node through different parent nodes at a certain probability, not only by selecting the preferred parent node, thus avoiding buffer overflow and reducing end-to-end delay. Finally, the two algorithms are combined to accommodate different application scenarios. The experimental results show that the proposed three improved schemes improve the reliability of the routing, extend the lifetime of the network, reduce the end-to-end delay, and reduce the number of DAG reconfigurations.
Composites for Timber-Replacement Bearers in Railway Switches and Crossings
Authors: Kaewunruen Sakdirat , You Ruilin , Ishida Makoto
Published: October 2017

Recent developments in composite materials have resulted in their pilot adoption in railway industry, such as ‘fibre-reinforced foamed urethane (FFU)’, ‘geopolymer concrete’, ‘recycled polymer’, and ‘CarbonLoc composite’. Railway track support systems are critical for safe and reliable operations of railway tracks. There are two types of support structures, which can be designed to be either a slab or a cluster of discrete bearers or sleepers. The choice of turnout support system depends on asset management strategy of the rail operators or maintainers. The aim of this paper is to present the criteria, fundamental and multi-disciplinary issues for the design and practical selection of composite materials in railway turnout systems. As a case study, a full-scale trial to investigate in-situ behaviours of a turnout grillage system using an alternative material, ‘fibre-reinforced foamed urethane (FFU)’ bearers, is presented. Influences of the composite bearers on track geometry (recorded by track inspection vehicle ‘AK Car’ and based on survey data), track settlement, track dynamics, and acoustic characteristics are highlighted in this paper. Comparative studies of composite materials for railway track applications are reviewed and presented in order to improve material design process. This state-of-the-art review paper will also focus on practicality and environmental risks of composite components in railway built environments. It embraces the requirement considerations of new materials for use as safety-critical track elements.
Multiple Roles of Pitx2 in Cardiac Development and Disease
Authors: Franco Diego , Sedmera David , Lozano-Velasco Estefanía
Published: October 2017

Cardiac development is a complex morphogenetic process initiated as bilateral cardiogenic mesoderm is specified at both sides of the gastrulating embryo. Soon thereafter, these cardiogenic cells fuse at the embryonic midline configuring a symmetrical linear cardiac tube. Left/right bilateral asymmetry is first detected in the forming heart as the cardiac tube bends to the right, and subsequently, atrial and ventricular chambers develop. Molecular signals emanating from the node confer distinct left/right signalling pathways that ultimately lead to activation of the homeobox transcription factor Pitx2 in the left side of distinct embryonic organ anlagen, including the developing heart. Asymmetric expression of Pitx2 has therefore been reported during different cardiac developmental stages, and genetic deletion of Pitx2 provided evidence of key regulatory roles of this transcription factor during cardiogenesis and thus congenital heart diseases. More recently, impaired Pitx2 function has also been linked to arrhythmogenic processes, providing novel roles in the adult heart. In this manuscript, we provide a state-of-the-art review of the fundamental roles of Pitx2 during cardiogenesis, arrhythmogenesis and its contribution to congenital heart diseases.
Diagnostic Accuracy of FebriDx: A Rapid Test to Detect Immune Responses to Viral and Bacterial Upper Respiratory Infections
Authors: Self Wesley , Rosen Jeffrey , Sharp Stephan , Filbin Michael , Hou Peter , Parekh Amisha , Kurz Michael , Shapiro Nathan.
Published: October 2017

C-reactive protein (CRP) and myxovirus resistance protein A (MxA) are associated with bacterial and viral infections, respectively. We conducted a prospective, multicenter, cross-sectional study of adults and children with febrile upper respiratory tract infections (URIs) to evaluate the diagnostic accuracy of a rapid CRP/MxA immunoassay to identify clinically significant bacterial infection with host response and acute pathogenic viral infection. The reference standard for classifying URI etiology was an algorithm that included throat bacterial culture, upper respiratory PCR for viral and atypical pathogens, procalcitonin, white blood cell count, and bandemia. The algorithm also allowed for physician override. Among 205 patients, 25 (12.2%) were classified as bacterial, 53 (25.9%) as viral, and 127 (62.0%) negative by the reference standard. For bacterial detection, agreement between FebriDx and the reference standard was 91.7%, with FebriDx having a sensitivity of 80% (95% CI: 59–93%), specificity of 93% (89–97%), positive predictive value (PPV) of 63% (45–79%), and a negative predictive value (NPV) of 97% (94–99%). For viral detection, agreement was 84%, with a sensitivity of 87% (75–95%), specificity of 83% (76–89%), PPV of 64% (63–75%), and NPV of 95% (90–98%). FebriDx may help to identify clinically significant immune responses associated with bacterial and viral URIs that are more likely to require clinical management or therapeutic intervention, and has potential to assist with antibiotic stewardship.
Fe Core–Carbon Shell Nanoparticles as Advanced MRI Contrast Enhancer
Authors: Chaudhary Rakesh , Kangasniemi Kim , Takahashi Masaya , Mohanty Samarendra , Koymen Ali
Published: October 2017

The aim of this study is to fabricate a hybrid composite of iron (Fe) core–carbon (C) shell nanoparticles with enhanced magnetic properties for contrast enhancement in magnetic resonance imaging (MRI). These new classes of magnetic core–shell nanoparticles are synthesized using a one-step top–down approach through the electric plasma discharge generated in the cavitation field in organic solvents by an ultrasonic horn. Transmission electron microscopy (TEM) observations revealed the core–shell nanoparticles with 10–85 nm in diameter with excellent dispersibility in water without any agglomeration. TEM showed the structural confirmation of Fe nanoparticles with body centered cubic (bcc) crystal structure. Magnetic multi-functional hybrid composites of Fe core–C shell nanoparticles were then evaluated as negative MRI contrast agents, displaying remarkably high transverse relaxivity (r2) of 70 mM−1·S−1 at 7 T. This simple one-step synthesis procedure is highly versatile and produces desired nanoparticles with high efficacy as MRI contrast agents and potential utility in other biomedical applications.
Characterizing the Performance of Gas-Permeable Membranes as an Ammonia Recovery Strategy from Anaerobically Digested Dairy Manure
Authors: Fillingham Melanie , VanderZaag Andrew , Singh Jessica , Burtt Stephen , Crolla Anna , Kinsley Chris , MacDonald J. Douglas
Published: October 2017

Capturing ammonia from anaerobically digested manure could simultaneously decrease the adverse effects of ammonia inhibition on biogas production, reduce reactive nitrogen (N) loss to the environment, and produce mineral N fertilizer as a by-product. In this study, gas permeable membranes (GPM) were used to capture ammonia from dairy manure and digestate by the diffusion of gaseous ammonia across the membrane where ammonia is captured by diluted acid, forming an aqueous ammonium salt. A lab-scale prototype using tubular expanded polytetrafluoroethylene (ePTFE) GPM was used to (1) characterize the effect of total ammonium nitrogen (TAN) concentration, temperature, and pH on the ammonia capture rate using GPM, and (2) to evaluate the performance of a GPM system in conditions similar to a mesophilic anaerobic digester. The GPM captured ammonia at a rate between 2.2 to 6.3% of gaseous ammonia in the donor solution per day. Capture rate was faster in anaerobic digestate than raw manure. The ammonia capture rate could be predicted using non-linear regression based on the factors of total ammonium nitrogen concentration, temperature, and pH. This use of membranes shows promise in reducing the deleterious impacts of ammonia on both the efficiency of biogas production and the release of reactive N to the environment.
Microstructure and Wear Resistance of Laser-Clad (Co, Ni)61.2B26.2Si7.8Ta4.8 Coatings
Authors: Zhang Luan , Wang Cunshan , Qian Shengnan , Yu Qun , Dong Chuang
Published: October 2017

It has been reported that a quaternary Co61.2B26.2Si7.8Ta4.8 alloy is a good glass former and can be laser-clad to an amorphous composite coating with superior hardness and wear resistance. In this paper, alloys with varying Ni contents to substitute for Co are coated on the surface of #45 carbon steel using a 5-kW CO2 laser source for the purpose of obtaining protective coatings. In contrast to the quaternary case, the clad layers are characterized by a matrix of α-(Fe, Co, Ni) solid solution plus CoB, Co3B, and Co3Ta types of precipitates. The cladding layer is divided into four regions: Near-surface dendrites, α-(Fe, Co, Ni) solid solution plus dispersed particles in the middle zone, columnar bonding zone, and heat-affected area that consists of martensite. The hardness gradually decreases with increasing Ni content, and the maximum hardness occurs in the middle zone. Both the friction coefficient and wear volume are minimized in the alloy containing 12.2% Ni. Compared with the previous cobalt-based quaternary alloy Co61.2B26.2Si7.8Ta4.8, the addition of the Ni element reduces the glass-forming ability and henceforth the hardness and wear resistance of the clad layers.
Solid-State Form Characterization of Riparin I
Authors: De Moura Elisana , Terto Márcio , De Moura Mendonça Elisângela , Procópio José , De O. Costa Vicente , Barbosa Filho José , Chavez Gutierrez Stanley , Tavares Josean , Oliveira Macedo Rui , Da Silva Marcelo
Published: October 2017

Riparin I is an alkamide with potential anxiolytic activity in preclinical studies. The characterization and understanding of solid-state properties play an importance role in drug development. For this work, the solid state of five riparin I batches (RIP-1, RIP-2, RIP-3, RIP-4, and RIP-5), obtained by the same synthesis process, were characterized by Scanning Electron Microscopy (SEM), Differential Scanning Calorimetry (DSC), DSC-photovisual, Thermogravimetry (TG), Fourier Transform Infrared (FTIR), Pyrolysis (Pyr-GC/MS), X-ray Powder Diffraction (PXRD), and Solid-State Nuclear Magnetic Resonance (ssNMR) techniques. Batches of riparin I with different crystal habits resulting in crystallization impurities were observed, which can be attributed to the presence of triethylamine. The main differences were observed by DSC, PXRD, and ssNMR analysis. DSC curves of RIP-2 and RIP-3 presented endothermic peaks at different temperatures of fusion, which can be attributed to the mixture of different crystalline forms. PXRD and ssNMR results confirmed crystallinity differences. The results offer evidence of the importance of controlling the reproducibility of the synthesis in order to obtain the adequate morphology for therapeutic efficacy and avoiding future problems in quality control of riparin I products.
Furanones and Anthranilic Acid Derivatives from the Endophytic Fungus Dendrothyrium variisporum
Authors: Teponno Rémy , Noumeur Sara , Helaly Soleiman , Hüttel Stephan , Harzallah Daoud , Stadler Marc
Published: October 2017

Extracts from an endophytic fungus isolated from the roots of the Algerian plant Globularia alypum showed prominent antimicrobial activity in a screening for novel antibiotics. The producer organism was identified as Dendrothyrium variisporum by means of morphological studies and molecular phylogenetic methods. Studies on the secondary metabolite production of this strain in various culture media revealed that the major components from shake flasks were massarilactones D (1) and H (2) as well as two new furanone derivatives for which we propose the trivial names (5S)-cis-gregatin B (3) and graminin D (4). Scale-up of the fermentation in a 10 L bioreactor yielded massarilactone D and several further metabolites. Among those were three new anthranilic acid derivatives (5–7), two known anthranilic acid analogues (8 and 9) and three cyclopeptides (10–12). Their structures were elucidated on the basis of extensive spectroscopic analysis (1D- and 2D-NMR), high-resolution mass spectrometry (HRESIMS), and the application of the modified Mosher’s method. The isolated metabolites were tested for antimicrobial and cytotoxic activities against various bacteria, fungi, and two mammalian cell lines. The new Metabolite 5 and Compound 9 exhibited antimicrobial activity while Compound 9 showed cytotoxicity activity against KB3.1 cells.
Flexible Piezoelectric Generators by Using the Bending Motion Method of Direct-Grown-PZT Nanoparticles on Carbon Nanotubes
Authors: Han Jin Kyu , Jeon Do Hyun , Cho Sam Yeon , Kang Sin Wook , Lim Jongsun , Bu Sang Don
Published: October 2017

Recently, composite-type nanogenerators (NGs) formed from piezoelectric nanostructures and multi-walled carbon nanotubes (CNTs), have become one of the excellent candidates for future energy harvesting because of their ability to apply the excellent electrical and mechanical properties of CNTs. However, the synthesis of NG devices with a high proportion of piezoelectric materials and a low polymer content, such as of polydimethylsiloxane (PDMS), continues to be problematic. In this work, high-piezoelectric-material-content flexible films produced from Pb(Zr,Ti)O3 (PZT)-atomically-interconnected CNTs and polytetrafluoroethylene (PTFE) are presented. Various physical and chemical characterization techniques are employed to examine the morphology and structure of the materials. The direct growth of the piezoelectric material on the CNTs, by stirring the PZT and CNT mixed solution, results in various positive effects, such as a high-quality dispersion in the polymer matrix and addition of flexoelectricity to piezoelectricity, resulting in the enhancement of the output voltage by an external mechanical force. The NGs repeatedly generate an output voltage of 0.15 V. These results present a significant step toward the application of NGs using piezoelectric nanocomposite materials.
Addendum: Shi, X.D.; Ruan, W.Q.; Hu, J.W.; Fan, M.Y.; Cao, R.S.; Wei, X.H. Optimizing the Removal of Rhodamine B in Aqueous Solutions by Reduced Graphene Oxide-Supported Nanoscale Zerovalent Iron (nZVI/rGO) Using an Artificial Neural Network-Genetic Algorithm (ANN-GA). Nanomaterials 2017, 7, 134
Authors: Shi Xuedan , Ruan Wenqian , Hu Jiwei , Fan Mingyi , Cao Rensheng , Wei Xionghui
Published: October 2017

The authors wish to make the following addendum to their paper [1]:[...]
Antioxidants Potential of the Filamentous Fungi (Mucor circinelloides)
Authors: Hameed Ahsan , Hussain Syed Ammar , Yang Junhuan , Ijaz Muhammad Umair , Liu Qing , Suleria Hafiz Ansar Rasul , Song Yuanda
Published: October 2017

Three important strains of Mucor circinelloides grown in complete and minimal media for specified period (72 h, 120 h and 168 h) under submerged fermentation conditions were investigated for their potential antioxidants/secondary metabolite production. All mycelial extracts demonstrated effective antioxidant activities in terms of β-carotene/linoleic acid bleaching, radical scavenging, reduction of metal ions and chelating abilities against ferrous ions. Different extraction methods and solvent systems affected the recovery yield and antioxidant activities of the extracts significantly (p ≤ 0.05). Ethanolic extracts were found to be rich source of antioxidant components and subsequently more effective in antioxidant properties. Fermentation period and media used also significantly affected (p ≤ 0.05) the antioxidant production and the resulting antioxidant properties. The (ethanolic) extracts of all the strains from late exponential growth phase (120 h) showed highest antioxidant production with topmost reducing, chelating and radical scavenging capabilities. Strain MC277.49 was found to be the highest producer of antioxidants followed by MC108.16 and WJ11. Phenolic compounds were detected significantly in higher (p ≤ 0.05) amount succeeded by the condensed tannins and flavonoids. Total phenol content of each extract was attributed to overall antioxidant capacity. Submerged fermentation with nutritional stress conditions were found to be excellent way of producing surplus amount of natural antioxidants/secondary metabolites with their vast potential commercial application in food and pharmaceutical industries.
Synthesis and Characterization of Multifunctional Two-Component Waterborne Polyurethane Coatings: Fluorescence, Thermostability and Flame Retardancy
Authors: Yin Xuan , Li Xiaoyu , Luo Yunjun
Published: October 2017

Fluorescent and flame-retardant two-component waterborne polyurethane coatings were synthesized using 1,5-dihydroxy naphthalene, a halogen-free polyphosphate and a hydrophilic curing agent, and their properties were systematically characterized. The average particle sizes and zeta potential values were below 170 nm and −30 mV. Meanwhile, the multifunctional two-component waterborne polyurethane coatings had strong fluorescence intensities. When comparing with the coatings with 0.5 wt % 1,5-dihydroxy naphthalene, the coatings with 1.0 wt % 1,5-dihydroxy naphthalene had a stronger microphase separation. Interestingly, the thermostability of the multifunctional coatings was remarkably improved through 1.0 wt % 1,5-dihydroxy naphthalene, and besides it belonged to nonflammable materials. Additionally, all of the coating films passed the solvent resistance testing. These samples with different amounts of 1,5-dihydroxy naphthalene are environmental friendly, especially applications that require transparent and fluorescent coatings.
Surface Properties of a Novel Poly(vinyl alcohol) Film Prepared by Heterogeneous Saponification of Poly(vinyl acetate) Film
Authors: Yang Seong , Yoo Sung , Lee Joon , Kim Jong , Yeum Jeong
Published: October 2017

Almost general poly(vinyl alcohol) (PVA) films were prepared by the processing of a PVA solution. For the first time, a novel poly(vinyl alcohol) (PVA) film was prepared by the saponification of a poly(vinyl acetate) (PVAc) film in a heterogenous medium. Under the same saponification conditions, the influence of saponification time on the degree of saponification (DS) was studied for the preparation of the saponified PVA film, and it was found that the DS varied with time. Optical microscopy was used to confirm the characteristics and surface morphology of the saponified PVA film, revealing unusual black globules in the film structure. The contact angle of the films was measured to study the surface properties, and the results showed that the saponified PVA film had a higher contact angle than the general PVA film. To confirm the transformation of the PVAc film to the PVA film, 1H nuclear magnetic resonance spectroscopy, X-ray diffraction measurements, differential scanning calorimetry, and Fourier-transform infrared spectroscopy were employed.
Block Copolymers: Synthesis, Self-Assembly, and Applications
Authors: Feng Hongbo , Lu Xinyi , Wang Weiyu , Kang Nam-Goo , Mays Jimmy
Published: October 2017

Research on block copolymers (BCPs) has played a critical role in the development of polymer chemistry, with numerous pivotal contributions that have advanced our ability to prepare, characterize, theoretically model, and technologically exploit this class of materials in a myriad of ways in the fields of chemistry, physics, material sciences, and biological and medical sciences. The breathtaking progress has been driven by the advancement in experimental techniques enabling the synthesis and characterization of a wide range of block copolymers with tailored composition, architectures, and properties. In this review, we briefly discussed the recent progress in BCP synthesis, followed by a discussion of the fundamentals of self-assembly of BCPs along with their applications.
Differential Proteome Analysis of Extracellular Vesicles from Breast Cancer Cell Lines by Chaperone Affinity Enrichment
Authors: Griffiths Steven , Cormier Michelle , Clayton Aled , Doucette Alan
Published: October 2017

The complexity of human tissue fluid precludes timely identification of cancer biomarkers by immunoassay or mass spectrometry. An increasingly attractive strategy is to primarily enrich extracellular vesicles (EVs) released from cancer cells in an accelerated manner compared to normal cells. The Vn96 peptide was herein employed to recover a subset of EVs released into the media from cellular models of breast cancer. Vn96 has affinity for heat shock proteins (HSPs) decorating the surface of EVs. Reflecting their cells of origin, cancer EVs displayed discrete differences from those of normal phenotype. GELFrEE LC/MS identified an extensive proteome from all three sources of EVs, the vast majority having been previously reported in the ExoCarta database. Pathway analysis of the Vn96-affinity proteome unequivocally distinguished EVs from tumorigenic cell lines (SKBR3 and MCF-7) relative to a non-tumorigenic source (MCF-10a), particularly with regard to altered metabolic enzymes, signaling, and chaperone proteins. The protein data sets provide valuable information from material shed by cultured cells. It is probable that a vast amount of biomarker identities may be collected from established and primary cell cultures using the approaches described here.
A Method for Enhancing the Sensing Distance of a Fingerprint Sensor
Authors: Song Kyung-Hoon , Choi Jaehuk , Chun Jung-Hoon
Published: October 2017

In this paper, we describe a method for improving the quality of fingerprint images at long sensing distances by reducing the fringe capacitance formed between a pixel and surrounding fingerprint patterns. Air-walls were applied to the glass attached to a fingerprint sensor for reducing the edge capacitance. Fingerprints and air-wall structures were modeled using a three-dimensional capacitance analysis tool. A prototype was fabricated by stacking glass layers with air-walls with a depth of 50 μm and a pitch of 50 μm on a silicon-type capacitive sensor. Using the results of simulations and prototype experiments, we confirmed that the proposed air-wall structure achieved high enough resolution to distinguish 2.5-lp/mm fingerprint patterns at a sensing distance of 300 μm or longer, and its contrast improved from 0.59 to 0.98.
Novel Semi-Parametric Algorithm for Interference-Immune Tunable Absorption Spectroscopy Gas Sensing
Authors: Michelucci Umberto , Venturini Francesca
Published: October 2017

One of the most common limits to gas sensor performance is the presence of unwanted interference fringes arising, for example, from multiple reflections between surfaces in the optical path. Additionally, since the amplitude and the frequency of these interferences depend on the distance and alignment of the optical elements, they are affected by temperature changes and mechanical disturbances, giving rise to a drift of the signal. In this work, we present a novel semi-parametric algorithm that allows the extraction of a signal, like the spectroscopic absorption line of a gas molecule, from a background containing arbitrary disturbances, without having to make any assumption on the functional form of these disturbances. The algorithm is applied first to simulated data and then to oxygen absorption measurements in the presence of strong fringes.To the best of the authors’ knowledge, the algorithm enables an unprecedented accuracy particularly if the fringes have a free spectral range and amplitude comparable to those of the signal to be detected. The described method presents the advantage of being based purely on post processing, and to be of extremely straightforward implementation if the functional form of the Fourier transform of the signal is known. Therefore, it has the potential to enable interference-immune absorption spectroscopy. Finally, its relevance goes beyond absorption spectroscopy for gas sensing, since it can be applied to any kind of spectroscopic data.
A Gas Chromatographic System for the Detection of Ethylene Gas Using Ambient Air as a Carrier Gas
Authors: Zaidi Nayyer Abbas , Tahir Muhammad Waseem , Vellekoop Michael J. , Lang Walter
Published: October 2017

Ethylene gas is a naturally occurring gas that has an influence on the shelf life of fruit during their transportation in cargo ships. An unintentional exposure of ethylene gas during transportation results in a loss of fruit. A gas chromatographic system is presented here for the detection of ethylene gas. The gas chromatographic system was assembled using a preconcentrator, a printed 3D printed gas chromatographic column, a humidity sensor, solenoid valves, and an electrochemical ethylene gas sensor. Ambient air was used as a carrier gas in the gas chromatographic system. The flow rate was fixed to 10 sccm. It was generated through a mini-pump connected in series with a mass flow controller. The metal oxide gas sensor is discussed with its limitation in ambient air. The results show the chromatogram obtained from metal oxide gas sensor has low stability, drifts, and has uncertain peaks, while the chromatogram from the electrochemical sensor is stable and precise. Furthermore, ethylene gas measurements at higher ppb concentration and at lower ppb concentration were demonstrated with the electrochemical ethylene gas sensor. The system separates ethylene gas and humidity. The chromatograms obtained from the system are stable, and the results are 1.2% repeatable in five similar measurements. The statistical calculation of the gas chromatographic system shows that a concentration of 2.3 ppb of ethylene gas can be detected through this system.
Gyroscope Technology and Applications: A Review in the Industrial Perspective
Authors: Passaro Vittorio M. N. , Cuccovillo Antonello , Vaiani Lorenzo , De Carlo Martino , Campanella Carlo Edoardo
Published: October 2017

This paper is an overview of current gyroscopes and their roles based on their applications. The considered gyroscopes include mechanical gyroscopes and optical gyroscopes at macro- and micro-scale. Particularly, gyroscope technologies commercially available, such as Mechanical Gyroscopes, silicon MEMS Gyroscopes, Ring Laser Gyroscopes (RLGs) and Fiber-Optic Gyroscopes (FOGs), are discussed. The main features of these gyroscopes and their technologies are linked to their performance.
High-Accuracy Decoupling Estimation of the Systematic Coordinate Errors of an INS and Intensified High Dynamic Star Tracker Based on the Constrained Least Squares Method
Authors: Jiang Jie , Yu Wenbo , Zhang Guangjun
Published: October 2017

Navigation accuracy is one of the key performance indicators of an inertial navigation system (INS). Requirements for an accuracy assessment of an INS in a real work environment are exceedingly urgent because of enormous differences between real work and laboratory test environments. An attitude accuracy assessment of an INS based on the intensified high dynamic star tracker (IHDST) is particularly suitable for a real complex dynamic environment. However, the coupled systematic coordinate errors of an INS and the IHDST severely decrease the attitude assessment accuracy of an INS. Given that, a high-accuracy decoupling estimation method of the above systematic coordinate errors based on the constrained least squares (CLS) method is proposed in this paper. The reference frame of the IHDST is firstly converted to be consistent with that of the INS because their reference frames are completely different. Thereafter, the decoupling estimation model of the systematic coordinate errors is established and the CLS-based optimization method is utilized to estimate errors accurately. After compensating for error, the attitude accuracy of an INS can be assessed based on IHDST accurately. Both simulated experiments and real flight experiments of aircraft are conducted, and the experimental results demonstrate that the proposed method is effective and shows excellent performance for the attitude accuracy assessment of an INS in a real work environment.
In-Season Yield Prediction of Cabbage with a Hand-Held Active Canopy Sensor
Authors: Ji Rongting , Min Ju , Wang Yuan , Cheng Hu , Zhang Hailin , Shi Weiming
Published: October 2017

Efficient and precise yield prediction is critical to optimize cabbage yields and guide fertilizer application. A two-year field experiment was conducted to establish a yield prediction model for cabbage by using the Greenseeker hand-held optical sensor. Two cabbage cultivars (Jianbao and Pingbao) were used and Jianbao cultivar was grown for 2 consecutive seasons but Pingbao was only grown in the second season. Four chemical nitrogen application rates were implemented: 0, 80, 140, and 200 kg·N·ha−1. Normalized difference vegetation index (NDVI) was collected 20, 50, 70, 80, 90, 100, 110, 120, 130, and 140 days after transplanting (DAT). Pearson correlation analysis and regression analysis were performed to identify the relationship between the NDVI measurements and harvested yields of cabbage. NDVI measurements obtained at 110 DAT were significantly correlated to yield and explained 87–89% and 75–82% of the cabbage yield variation of Jianbao cultivar over the two-year experiment and 77–81% of the yield variability of Pingbao cultivar. Adjusting the yield prediction models with CGDD (cumulative growing degree days) could make remarkable improvement to the accuracy of the prediction model and increase the determination coefficient to 0.82, while the modification with DFP (days from transplanting when GDD > 0) values did not. The integrated exponential yield prediction equation was better than linear or quadratic functions and could accurately make in-season estimation of cabbage yields with different cultivars between years.
Adaptive Integration of the Compressed Algorithm of CS and NPC for the ECG Signal Compressed Algorithm in VLSI Implementation
Authors: Tseng Yun-Hua , Chen Yuan-Ho , Lu Chih-Wen
Published: October 2017

Compressed sensing (CS) is a promising approach to the compression and reconstruction of electrocardiogram (ECG) signals. It has been shown that following reconstruction, most of the changes between the original and reconstructed signals are distributed in the Q, R, and S waves (QRS) region. Furthermore, any increase in the compression ratio tends to increase the magnitude of the change. This paper presents a novel approach integrating the near-precise compressed (NPC) and CS algorithms. The simulation results presented notable improvements in signal-to-noise ratio (SNR) and compression ratio (CR). The efficacy of this approach was verified by fabricating a highly efficient low-cost chip using the Taiwan Semiconductor Manufacturing Company’s (TSMC) 0.18-μm Complementary Metal-Oxide-Semiconductor (CMOS) technology. The proposed core has an operating frequency of 60 MHz and gate counts of 2.69 K.
An Efficient and QoS Supported Multichannel MAC Protocol for Vehicular Ad Hoc Networks
Authors: Song Caixia , Tan Guozhen , Yu Chao
Published: October 2017

Vehicular Ad Hoc Networks (VANETs) employ multichannel to provide a variety of safety and non-safety (transport efficiency and infotainment) applications, based on the IEEE 802.11p and IEEE 1609.4 protocols. Different types of applications require different levels Quality-of-Service (QoS) support. Recently, transport efficiency and infotainment applications (e.g., electronic map download and Internet access) have received more and more attention, and this kind of applications is expected to become a big market driver in a near future. In this paper, we propose an Efficient and QoS supported Multichannel Medium Access Control (EQM-MAC) protocol for VANETs in a highway environment. The EQM-MAC protocol utilizes the service channel resources for non-safety message transmissions during the whole synchronization interval, and it dynamically adjusts minimum contention window size for different non-safety services according to the traffic conditions. Theoretical model analysis and extensive simulation results show that the EQM-MAC protocol can support QoS services, while ensuring the high saturation throughput and low transmission delay for non-safety applications.
Divergent Developmental Trajectories and Strategic Coupling in the Pearl River Delta: Where Is a Sustainable Way of Regional Economic Growth?
Authors: Liu Yi , Liang Yutian , Ma Shiping , Huang Kaixuan
Published: October 2017

This paper interprets regional economic sustainability in the context of the globalization of late-coming regions. Drawing upon the concept of strategic coupling from economic geography, this paper proposes two types of strategic coupling, captive and proactive coupling, for better understanding regional sustainability and resilience through the experiences of the Pearl River Delta in China. It finds that sub-regional economies under captive coupling become highly dependent on exogenous growth and are vulnerable to external shocks. This trajectory looks less sustainable according to the general understanding, but it interestingly shows better resilience during and after the 2008 global financial crisis. In contrast, the ones under proactive coupling are less volatile, but growing much slower and are less resilient. By reporting these regional economic dynamics, this paper argues that sustainability in late-coming regions cannot be explained by either intra-regional forces or the means of global integration alone. In contrast, it has to be explained by the combination of both; the alleged strategic coupling in which economic growth and learning happens. This paper thus calls for greater attention to strategic coupling, the trade-off of globalization and resilience for understanding regional sustainability, rather than purely focusing on resource utilization and ecological balance.
Technology Commercialization through Sustainable Knowledge Sharing from University-Industry Collaborations, with a Focus on Patent Propensity
Authors: Han Junghee
Published: October 2017

The process of technology commercialization involves numerous hurdles, from the original idea to its translation into the market. Patenting brings to light the research activities created through university-industry collaborations. This paper explores patent propensity, as related to commercializing innovative activities resulting from collaborations between universities and regional small and medium enterprises (SMEs). To achieve our aims, we apply a robust regression analysis to test five research hypotheses using firm-level data on 263 firms located in the Gwangju region of Korea. Our empirical results show that certain industry characteristics are negatively related to the propensity to patent. In addition, and contrary to expectations, it is found that the InnoBiz firms that the government designated as innovative SMEs are not performing any differently from general firms. It is only the CEO’s academic credentials that are found to be positively related to propensity to patent. From the findings, we can conclude that patenting propensity is likely related to CEOs’ managerial strategies rather than the particular characteristics of the technology in question. We also suggest new technology commercialization related with universities’ policies and support to promote regional SMEs including conglomerated firms as well as universities.
Performance Evaluation and Life Cycle Cost Analysis Model of a Gondola-Type Exterior Wall Painting Robot
Authors: Yeom Dong-Jun , Na Eun-Ji , Lee Mi-Young , Kim Yoo-Jun , Kim Young , Cho Chung-Suk
Published: October 2017

The amount and market size of apartment complex exterior wall painting work continues to increase each year in South Korea. Nevertheless, there are difficulties with the supply and demand of human resources due to the high risks associated with conventional painting work. To resolve these issues, research and development has recently been conducted on a Gondola-type Exterior Wall Painting robot (GEWPro). The aims of this study were to develop a performance evaluation and life cycle cost (LCC) analysis model for a GEWPro and deduce its performance and economic efficiency through a case study. According to the results, the performance of the automated method was 16.8% higher than that of the conventional method, and the economic efficiency was also superior (benefit/cost ratio 6.39). These results show that the proposed performance evaluation and LCC analysis model can predict the productivity and economic efficiency of automated methods.
Sustainable Production Line Evaluation Based on Evidential Reasoning
Authors: Zhou Zhexuan , Dou Yajie , Sun Jianbin , Jiang Jiang , Tan Yuejin
Published: October 2017

Many production line imbalances have been observed in the pursuit of higher profits. A sustainable production line, also called balanced, leads to lower costs, good production environments, and green manufacturing. A decision analysis method, such as production line evaluation, is often employed to help decision makers make sustainable decisions. In this study, a sustainable decision-making model is proposed for the evaluation of engine manufacturing. To solve uncertainties in manufacturing industries while maintaining lower costs and an efficient production environment, evidential reasoning is used in order to evaluate the sustainable production line effectively. First, uncertainties in the engine production line and deficiencies in the existing methods for evaluating the sustainable production line are analyzed. Then, evidential reasoning evaluation of the sustainable engine production line model is proposed and an example is presented; to be specific, the analysis of three production line plans is conducted using evidential reasoning, and plan P3 is found to be the best. Finally, a FlexSim simulation is used to prove the feasibility of evidential reasoning evaluation, verifying its suitability for achieving sustainable production line evaluation.
Political Connections, Government Subsidies and Technical Innovation of Wind Energy Companies in China
Authors: Qu Jiaan , Cao Jie , Wang Xinting , Tang Jiexin , Bukenya James O.
Published: October 2017

Developing wind energy is one of the win win measures in response to climate changes and energy security. In order to promote technical innovation in the wind-energy industry, the government grants various fiscal subsidies to wind-energy companies every year. To acquire these subsidies, enterprises often employ those with political backgrounds as members of the board of directors and board of supervisors. On the one hand, the acquisition of subsidies may indeed promote the technical innovation capacity of enterprises, but, on the other hand, due to the existence of “the grabbing hand”, the technical innovation capacity of enterprises may be weakened. We selected 35 Chinese wind-energy listed companies to analyze the relationship between political connections, subsidies and the technical innovation capacity. Results indicate that, political connections to an enterprise weaken its innovative potential and achievement. Moreover, the higher the strength of political connections is, the stronger the negative impact it will bring to the innovative capacity of the enterprise. Modulation of government subsidies, however, can alleviate the negative effects of political connections.
Bushfire Disaster Monitoring System Using Low Power Wide Area Networks (LPWAN)
Authors: Kang James , Adibi Sasan
Published: October 2017

Some applications, including disaster monitoring and recovery networks, use low-power wide-area networks (LPWAN). LPWAN sensors capture data bits and transmit them to public carrier networks (e.g., cellular networks) via dedicated gateways. One of the challenges encountered in disaster management scenarios revolves around the carry/forward sensed data and geographical location information dissemination to the disaster relief operatives (disaster relief agency; DRA) to identify, characterise, and prioritise the affected areas. There are network topology options to reach its destination, including cellular, circuit switched, and peer-to-peer networks. In the context of natural disaster prediction, it is vital to access geographical location data as well as the timestamp. This paper proposes the usage of Pseudo A Number (PAN), that is, the calling party address, which is used by every network to include the location information instead of the actual calling party address of the gateway in LPWAN. This PAN information can be further analysed by the DRA to identify the affected areas and predict the complications of the disaster impacts in addition to the past history of damages. This paper aims to propose a solution that can predict disaster proceedings based on propagation and the velocity of impact using vector calculation of the location data and the timestamp, which are transmitted by sensors through the PAN of the gateway in LPWAN.