bluuebunny/crossref_metadata_2025 huggingface dataset:
Empirical Study on CO2 Emissions, Financial Development and Economic Growth of the BRICS Countries
Authors: Li Fangjhy , Wu Yang-Che , Wang Mei-Chih , Wong Wing-Keung , Xing Zhijie
Published: November 2021

This paper empirically examined relevant data on BRICS CO2 emissions, financial development, and economic growth in the past 40 years, and analyzed the correlation between them. Using the cointegration test, it found that there is a clear correlation between the variables in China and South Africa, which show that there is a two-way relationship between CO2 emissions, financial development, and economic growth in both countries. Using the quantile regression method in the analysis, the results demonstrated that at the 0.6th quartile, South Africa’s financial development had a negative impact on CO2 emissions, while Brazil’s CO2 emissions had a negative impact on financial development. Economic growth was subsequently added as a control variable, and the quantile-on-quantile regression method was used to test the correlation between the financial development of the BRICS countries and their CO2 emissions. Finally, based on empirical conclusions, this paper proposed that BRICS countries should focus on sustainable economic development; when government departments formulate emission-reduction policies, they must reasonably consider the relationship between financial development and emission-reduction policies.
Alphonse II of Aragon (1164–1196)
Authors: Serrano-Coll Marta
Published: November 2021

Alphonse II King of Aragon (1164–1196). He was the first king of the Crown of Aragon and son of the Queen Petronila of Aragon (1157–1164) and the count of Barcelona, Ramon Berenguer IV (1137–1162). Aware of the new political reality that he embodied as King of Aragon and Count of Barcelona, Alphonse II made significant changes to his iconography. Among the most important of these is the binomial that he incorporated to his pendent seals; that is, a portrayal of Alphonse enthroned as king on the obverse and Alphonse as count and mounted on a horse on the reverse. As a known bibliophile and as a result of his desire to reorganise his chancellery following the union of various political entities, he ordered the compilation of the Liber Feudorum Maior, the folios of which demonstrate his potestas regia through their lavish iconography. He was no less innovative in his coinage, on which he included, for the first time, the image of his head wearing the crown.
Using TLS-Measured Tree Attributes to Estimate Aboveground Biomass in Small Black Spruce Trees
Authors: Wagers Steven , Castilla Guillermo , Filiatrault Michelle , Sanchez-Azofeifa G. Arturo
Published: November 2021

Research Highlights: This study advances the effort to accurately estimate the biomass of trees in peatlands, which cover 13% of Canada’s land surface. Background and Objectives: Trees remove carbon from the atmosphere and store it as biomass. Terrestrial laser scanning (TLS) has become a useful tool for modelling forest structure and estimating the above ground biomass (AGB) of trees. Allometric equations are often used to estimate individual tree AGB as a function of height and diameter at breast height (DBH), but these variables can often be laborious to measure using traditional methods. The main objective of this study was to develop allometric equations using TLS-measured variables and compare their accuracy with that of other widely used equations that rely on DBH. Materials and Methods: The study focusses on small black spruce trees (<5 m) located in peatland ecosystems of the Taiga Plains Ecozone in the Northwest Territories, Canada. Black spruce growing in peatlands are often stunted when compared to upland black spruce and having models specific to them would allow for more precise biomass estimates. One hundred small trees were destructively sampled from 10 plots and the dry weight of each tree was measured in the lab. With this reference data, we fitted biomass models specific to peatland black spruce using DBH, crown diameter, crown area, height, tree volume, and bounding box volume as predictors. Results: Our best models had crown size and height as predictors and outperformed established AGB equations that rely on DBH. Conclusions: Our equations are based on predictors that can be measured from above, and therefore they may enable the plotless creation of accurate biomass reference data for a prominent tree species in a common ecosystem (treed peatlands) in North America’s boreal.
A Simulation-Based Evaluation of a Cargo-Hitching Service for E-Commerce Using Mobility-on-Demand Vehicles
Authors: Romano Alho André , Sakai Takanori , Oh Simon , Cheng Cheng , Seshadri Ravi , Chong Wen Han , Hara Yusuke , Caravias Julia , Cheah Lynette , Ben-Akiva Moshe
Published: November 2021

Time-sensitive parcel deliveries—shipments requested for delivery in a day or less—are an increasingly important aspect of urban logistics. It is challenging to deal with these deliveries from a carrier perspective. These require additional planning constraints, preventing the efficient consolidation of deliveries that is possible when demand is well known in advance. Furthermore, such time-sensitive deliveries are requested to a wider spatial scope than retail centers, including homes and offices. Therefore, an increase in such deliveries is considered to exacerbate negative externalities, such as congestion and emissions. One of the solutions is to leverage spare capacity in passenger transport modes. This concept is often denominated as cargo hitching. While there are various system designs, it is crucial that such a solution does not deteriorate the quality of service of passenger trips. This research aims to evaluate the use of mobility-on-demand (MOD) services that perform same-day parcel deliveries. To test the MOD-based solutions, we utilize a high-resolution agent- and activity-based simulation platform of passenger and freight flows. E-commerce demand carrier data collected in Singapore are used to characterize simulated parcel delivery demand. We explore operational scenarios that aim to minimize the adverse effects of fulfilling deliveries with MOD service vehicles on passenger flows. Adverse effects are measured in fulfillment, wait, and travel times. A case study on Singapore indicates that the MOD services have potential to fulfill a considerable amount of parcel deliveries and decrease freight vehicle traffic and total vehicle kilometers travelled without compromising the quality of MOD for passenger travel. Insights into the operational performance of the cargo-hitching service are also provided.
Third-Generation Calibrations for MeerKAT Observation
Authors: Parekh Viral , Kincaid Robert , Hugo Benjamin , Ramaila Athanaseus , Oozeer Nadeem
Published: November 2021

Superclusters and galaxy clusters offer a wide range of astrophysical science topics with regards to studying the evolution and distribution of galaxies, intra-cluster magnetization mediums, cosmic ray accelerations and large scale diffuse radio sources all in one observation. Recent developments in new radio telescopes and advanced calibration software have completely changed data quality that was never possible with old generation telescopes. Hence, radio observations of superclusters are a very promising avenue to gather rich information of a large-scale structure (LSS) and their formation mechanisms. These newer wide-band and wide field-of-view (FOV) observations require state-of-the-art data analysis procedures, including calibration and imaging, in order to provide deep and high dynamic range (DR) images with which to study the diffuse and faint radio emissions in supercluster environments. Sometimes, strong point sources hamper the radio observations and limit the achievement of a high DR. In this paper, we have shown the DR improvements around strong radio sources in the MeerKAT observation of the Saraswati supercluster by applying newer third-generation calibration (3GC) techniques using CubiCal and killMS software. We have also calculated the statistical parameters to quantify the improvements around strong radio sources. This analysis advocates for the use of new calibration techniques to maximize the scientific returns from new-generation telescopes.
Screening of Amaranthus sp. Varieties for Resistance to Bacterial Wilt Caused by Ralstonia solanacearum
Authors: Sikirou Rachidatou , Dossoumou Marie Epiphane , Honfoga Judith , Afari-Sefa Victor , Srinivasan Ramasamy , Paret Mathews , Bihon Wubetu
Published: November 2021

Bacterial wilt, caused by Ralstonia solanacearum, is an emerging constraint in amaranth production in Benin. Host resistance is the most sustainable disease control measure. Ten amaranth varieties including A2002, Bresil (B) -Sel, Madiira 2, AC-NL, GARE ES13-7, Madiira 1, UG-AMES13-2, AM-NKGN, IP-5-Sel and a local variety from Benin were screened for resistance to bacterial wilt. The study was conducted in a screen house and in the naturally contaminated open field during a consecutive rainy and dry season using a randomized complete block design with four and three replications, respectively. In the screen house, plants were inoculated by drenching a 40 mL of bacterial suspension containing 108 CFU/mL of R. solanacearum strain NCBI 5 GenBank N° MH397250 at the collar region. The bacterial wilt incidence (BWI) and the area under the disease progress curve (AUDPC) suggested differential reactions of amaranth varieties to the pathogen. BWI and AUDPC were low for UG-AMES13-2, moderate for Madiira 2, AM-NKGN and the local variety and very high for A2002, Bresil (B) -Sel, AC-NL, GARE ES13-7, Madiira 1 and IP-5-Sel. The World Vegetable Center’s UG-AMES13-2 can be considered as first choice, which is resistant to R. solanacearum, and should be scaled up for seed production towards supporting farmers.
Preharvest Foliar Spray of Calcium Chloride on Growth, Yield, Quality, and Shelf Life Extension of Different Lowland Tomato Varieties in Malaysia
Authors: Mazumder Mohammad Nurun Nabi , Misran Azizah , Ding Phebe , Wahab Puteri Edaroyati Megat , Mohamad Azhar
Published: November 2021

Tomato is a popularly consumed vegetable fruit and suffers from huge losses due to its high perishability. Calcium chloride (CaCl2∙2H2O) application has been shown to be an important method that can extend the shelf life of tomato fruits. The current study aimed to determine the effect of pre-harvest treatments of CaCl2 on the growth, yield, quality, and shelf-life performance of tomato varieties. Four tomato varieties known as MT-1, MT-3, 303, and 105 were sprayed with the CaCl2 solutions (0.0%, 1.0%, 1.5%, and 2.0%, w/v) after seven days of fruit initiation and were sprayed every week until the first harvest. Spraying with 2% of CaCl2 showed an improvement in controlling physiological disorders such as blossom end rot (BER), weight loss, declined disease incidence, and disease severity. An increase in fruit quality was also observed as the treated fruit could maintain its shelf life up to 20 days at ambient conditions. At the highest doses of CaCl2 (2%), it increased the total ascorbic acid (AA), lycopene content, total phenolic content (TPC), and antioxidant activity (DPPH) in comparison to lower doses and control tomatoes. MT-3 responded well to higher doses of CaCl2 (2%) among the four tomato varieties and performed better in growth, yield, disease, insect infestation, quality, and shelf-life performance compared to the other varieties and CaCl2 treatment combination.
Molecular Insights into the Effects of Rootstocks on Maturation of Blood Oranges
Authors: Lana Giuseppe , Modica Giulia , Las Casas Giuseppina , Siracusa Laura , La Malfa Stefano , Gentile Alessandra , Sicilia Angelo , Distefano Gaetano , Continella Alberto
Published: November 2021

Rootstock choice has important effects on the horticultural and pathological traits of the citrus cultivars. Thus, the scion/rootstock combination can affect tree vigour, nutrition, and stress resistance; it can also have positive influences on the fruit quality traits. Although the study of rootstock effects has been a relevant research topic in citrus for many years, the main body of such study has been conducted at the biochemical level, while little effort has been directed to the determination of the rootstock influences at the molecular level. A comparative study of three combinations of scion and rootstock shows a positive correlation between the regulation of the fruit quality-related genes and the accumulations of bioactive compounds, as well as with acid degradation. Monitoring the anthocyanin accumulation during ripening shows the scion/rootstock combination can increase anthocyanin synthesis in the fruit, as well as vitamin C accumulation and acid degradation. Our results show that the rootstock genotype can exert important influences on citrus fruit quality by affecting gene expression in the scion. New insights into the molecular interactions between scion and rootstock may help unravel the systems through which rootstocks exert their influences on the regulatory networks in the scion, so as to influence relevant agronomic traits. This information should result in an improved rootstock breeding selection and definition of scion/rootstock combinations to enhance fruit quality traits.
Hydroclimatological Patterns and Limnological Characteristics of Unique Wetland Systems on the Argentine High Andean Plateau
Authors: Frau Diego , Moran Brendan J. , Arengo Felicity , Marconi Patricia , Battauz Yamila , Mora Celeste , Manzo Ramiro , Mayora Gisela , Boutt David F.
Published: November 2021

High-elevation wetlands in South America are not well described despite their high sensitivity to human impact and unique biodiversity. We describe the hydroclimatological and limnological characteristics of 21 wetlands on the High Andean Plateau of Argentina, synthesizing information gathered over ten years (2010–2020). We collected physical-chemical, phytoplankton, and zooplankton data and counted flamingos in each wetland. We also conducted an extensive analysis of climatic patterns and hydrological responses since 1985. These wetlands are shallow, with a wide range of salinity (from fresh to brine), mostly alkaline, and are dominated by carbonate and gypsum deposits and sodium-chloride waters. They tend to have high nutrient concentrations. Plankton shows a low species richness and moderate to high dominance of taxa. Flamingos are highly dependent on the presence of Bacillariophyta, which appears to be positively linked to silica and soluble reactive phosphorus availability. Climatic conditions show a strong region-wide increase in average air temperature since the mid-1980s and a decrease in precipitation between 1985–1999 and 2000–2020. These high-elevation wetlands are fundamentally sensitive systems; therefore, having baseline information becomes imperative to understanding the impact of climatic changes and other human perturbations. This work attempts to advance the body of scientific knowledge of these unique wetland systems.
Determinants of Consumers’ Trust in Biotech Brands and Purchase Intentions towards the Cord Blood Products
Authors: Chen Shih-Wei , Lee Ku-Yuan , Hsieh Chi-Ming
Published: November 2021

The development of the biotech industry is in full swing, and consumers have begun to value biotech brands. Since biotech products often focus on the future or special benefits, consumers inevitably bear certain risks when purchasing biotech products, and their trust in the biotech brand will have an important impact on their purchase intention. Previous studies have lacked a targeted understanding of consumer trust in biotech brands and a discussion of cultural viewpoints. This study introduced the concept of personal connections in Chinese relationalism and trust strategies in Chinese society to address this gap. In-depth interviews and focus group discussions were conducted in collaboration with Company X, a listed Taiwanese cord blood company, to extract the key factors influencing consumer trust and purchase intention of biotech brands. After constructing the structure model, the study was validated using a structural equation model through investigation and survey. The findings indicated that consumer trust in biotech brands was constructed by a combination of kinship trust transfer and emergent trust transfer within the consumer relationship network, as well as institutional trust and professional trust outside the relationship network and that a significant positive correlation existed between consumer trust in biotech brands and purchase intention. The acquaintances within the consumer relationship network include not only relatives and friends but also health care workers and netizens that consumers come into contact with. In addition, kinship trust transfer and emergent trust transfer within the consumer relationship network have a greater impact on trust in biotech brands than the institutional trust and professional trust outside the relationship network. The findings of this study deepen the understanding of consumer trust in biotech brands across cultures, and suggest that future marketing communication should be expanded to include key players within the consumer relationship network.
Recreational Exercising and Self-Reported Cardiometabolic Diseases in German People Living with HIV: A Cross-Sectional Study
Authors: Pérez-Chaparro Camilo Germán Alberto , Schuch Felipe B. , Zech Philipp , Kangas Maria , Rapp Michael A. , Heissel Andreas
Published: November 2021

Exercise is known for its beneficial effects on preventing cardiometabolic diseases (CMDs) in the general population. People living with the human immunodeficiency virus (PLWH) are prone to sedentarism, thus raising their already elevated risk of developing CMDs in comparison to individuals without HIV. The aim of this cross-sectional study was to determine if exercise is associated with reduced risk of self-reported CMDs in a German HIV-positive sample (n = 446). Participants completed a self-report survey to assess exercise levels, date of HIV diagnosis, CD4 cell count, antiretroviral therapy, and CMDs. Participants were classified into exercising or sedentary conditions. Generalized linear models with Poisson regression were conducted to assess the prevalence ratio (PR) of PLWH reporting a CMD. Exercising PLWH were less likely to report a heart arrhythmia for every increase in exercise duration (PR: 0.20: 95% CI: 0.10–0.62, p < 0.01) and diabetes mellitus for every increase in exercise session per week (PR: 0.40: 95% CI: 0.10–1, p < 0.01). Exercise frequency and duration are associated with a decreased risk of reporting arrhythmia and diabetes mellitus in PLWH. Further studies are needed to elucidate the mechanisms underlying exercise as a protective factor for CMDs in PLWH.
Against the Odds: A Structural Equation Analysis of Family Resilience Processes during Paternal Incarceration
Authors: Morgan Amy A. , Arditti Joyce A. , Dennison Susan , Frederiksen Signe
Published: November 2021

On any given day, approximately 2.1 million children in Europe have an incarcerated parent. Although research indicates that material hardship is associated with parental incarceration, and particularly paternal incarceration, little is known about family processes that may mitigate the harmful effects of such hardship on children with an incarcerated parent. Guided by a resilience framework, this study examined how family processes mediate the effects of material hardship on youth academic adjustment within the context of paternal incarceration. Using Danish data that assessed key family constructs, structural equation modeling was used to perform a mediational within-group analysis of primary caregivers (n = 727) to children with an incarcerated father. Results indicate that although social support and parenting skills did not yield mediating effects, caregiver mental health strongly mediated the effects of material hardship on youth academic adjustment during paternal incarceration. Findings suggest that economic conditions, as well as caregiver mental health symptoms, are important areas of intervention that may promote family-level resilience for youth of an imprisoned father. We conclude with research and practice recommendations to advance our understanding of resilience among families with an incarcerated parent.
The Influence of Atopic Dermatitis on Health-Related Quality of Life in Bangladesh
Authors: Nagata Abir , Kazi Taheruzzaman , Akter Zubaida , Nody Fariha Afrin , Khan Mohammad Shahriar , Shahriar Abu Saleh Muhammad , Islam Md Sayeedul , Nakagawa Takatoshi , Inui Shigeki
Published: November 2021

Atopic dermatitis (AD) is the foremost non-fatal skin-related disease that affects all age groups. Despite the growing prevalence of AD in low- and middle-income countries, its physiological consequences remain overlooked in countries like Bangladesh. Therefore, we aim to assess and characterize the influence of AD on the health-related quality of life (HRQoL) in Bangladeshi patients. A cross-sectional study comprising 184 eligible adults (83 men and 101 women; mean age, 33.46 ± 15.44 years) was conducted at the dermatology outpatient department of Shaheed Suhrawardy Medical College Hospital (a tertiary hospital in Dhaka, Bangladesh). AD was determined using the UK Working Party criteria. A structured questionnaire, Eczema Area and Severity Index (EASI), and Dermatology Life Quality Index (DLQI) were administered to obtain information on patient characteristics, AD severity, and HRQoL. The mean DLQI score for the entire sample was 11.29 ± 5.27 (range, 1–26), and 51.60% reported the disease greatly affected their lives. Bivariate analysis revealed significant differences in self-rated health measures of DLQI scores in terms of self-reported AD severity, overall health, and the EASI. In multivariable regression models adjusted for patient characteristics, the self-perceived severe AD group reported significantly higher DLQI scores (coefficient = 2.72; 95% confidence interval (CI) = 0.38–5.05; p = 0.022) than the mild group. Concurrently, we observed a substantial increase in the DLQI scores among patients with moderate and severe EASI scores (coefficient = 1.96, 95% CI = 0.08–3.92, p < 0.05 and coefficient = 4.35, 95% CI = 1.98–6.72, p < 0.001, respectively) than in those with mild EASI scores, suggesting that HRQoL was markedly influenced by greater AD severity. These findings highlight the need for a more patient-centric approach to the management of AD in order to alleviate patient suffering and, thereby, improve HRQoL.
The Role of Controlled Motivation in the Self-Esteem of Adolescent Students in Physical Education Classes
Authors: Valero-Valenzuela Alfonso , Huéscar Elisa , Núñez Juan L. , León Jaime , Conte Luis , Moreno-Murcia Juan Antonio
Published: November 2021

The aim of this cross-sectional study was to analyse the relationships between the satisfaction of psychological basic needs, physical education, academic controlling motivation, and self-esteem, and to propose a prediction model in line with the postulates from the hierarchical model found in the self-determination theory. The participants were 618 physical education students from primary and secondary school (317 girls and 301 boys) aged between 10 and 14 years old (M = 11.62; SD = 0.94). The questionnaires basic psychological needs in exercise measurement scale (BPNES), perceived locus of causality scale (PLOC), the academic motivation scale (EME), and physical self-perception profile (PSPP) were used to measure the studied variables. The results showed that autonomy and relatedness significantly and negatively predicted physical education controlling motivation, which predicted a positive and significant academic controlling motivation. This, in turn, negatively and significantly predicted self-esteem. It is concluded that it is essential to avoid controlling motivation to promote the development of a positive self-perception in students.
Gender Differences and Immunotherapy Outcome in Advanced Lung Cancer
Authors: Vavalà Tiziana , Catino Annamaria , Pizzutilo Pamela , Longo Vito , Galetta Domenico
Published: November 2021

In developed countries, lung cancer is the leading cause of cancer-related death in both sexes. Although cigarette smoking represents the principal risk factor for lung cancer in females, the higher proportion of this neoplasm among non-smoking women as compared with non-smoking men implies distinctive biological aspects between the two sexes. Gender differences depend not only on genetic, environmental, and hormonal factors but also on the immune system, and all these aspects are closely interconnected. In the last few years, it has been confirmed that the immune system plays a fundamental role in cancer evolution and response to oncological treatments, specifically immunotherapy, with documented distinctions between men and women. Consequently, in order to correctly assess cancer responses and disease control, considering only age and reproductive status, the results of studies conducted in female patients would probably not categorically apply to male patients and vice versa. The aim of this article is to review recent data about gender disparities in both healthy subjects’ immune system and lung cancer patients; furthermore, studies concerning gender differences in response to lung cancer immunotherapy are examined.
Hierarchically Structured Polystyrene-Based Surfaces Amplifying Fluorescence Signals: Cytocompatibility with Human Induced Pluripotent Stem Cell
Authors: Skopalová Kateřina , Radaszkiewicz Katarzyna Anna , Kadlečková Markéta , Pacherník Jiří , Minařík Antonín , Capáková Zdenka , Kašpárková Věra , Mráček Aleš , Daďová Eliška , Humpolíček Petr
Published: November 2021

An innovative multi-step phase separation process was used to prepare tissue culture for the polystyrene-based, hierarchically structured substrates, which mimicked in vivo microenvironment and architecture. Macro- (pore area from 3000 to 18,000 µm2; roughness (Ra) 7.2 ± 0.1 µm) and meso- (pore area from 50 to 300 µm2; Ra 1.1 ± 0.1 µm) structured substrates covered with micro-pores (area around 3 µm2) were prepared and characterised. Both types of substrate were suitable for human-induced pluripotent stem cell (hiPSC) cultivation and were found to be beneficial for the induction of cardiomyogenesis in hiPSC. This was confirmed both by the number of promoted proliferated cells and the expressions of specific markers (Nkx2.5, MYH6, MYL2, and MYL7). Moreover, the substrates amplified the fluorescence signal when Ca2+ flow was monitored. This property, together with cytocompatibility, make this material especially suitable for in vitro studies of cell/material interactions within tissue-mimicking environments.
Spike Formation Is a Turning Point Determining Wheat Root Microbiome Abundance, Structures and Functions
Authors: Usyskin-Tonne Alla , Hadar Yitzhak , Minz Dror
Published: November 2021

Root selection of their associated microbiome composition and activities is determined by the plant’s developmental stage and distance from the root. Total gene abundance, structure and functions of root-associated and rhizospheric microbiomes were studied throughout wheat growth season under field conditions. On the root surface, abundance of the well-known wheat colonizers Proteobacteria and Actinobacteria decreased and increased, respectively, during spike formation, whereas abundance of Bacteroidetes was independent of spike formation. Metagenomic analysis combined with functional co-occurrence networks revealed a significant impact of plant developmental stage on its microbiome during the transition from vegetative growth to spike formation. For example, gene functions related to biofilm and sensorial movement, antibiotic production and resistance and carbons and amino acids and their transporters. Genes associated with these functions were also in higher abundance in root vs. the rhizosphere microbiome. We propose that abundance of transporter-encoding genes related to carbon and amino acid, may mirror the availability and utilization of root exudates. Genes related to antibiotic resistance mechanisms were abundant during vegetative growth, while after spike formation, genes related to the biosynthesis of various antibiotics were enriched. This observation suggests that during root colonization and biofilm formation, bacteria cope with competitor’s antibiotics, whereas in the mature biofilm stage, they invest in inhibiting new colonizers. Additionally, there is higher abundance of genes related to denitrification in rhizosphere compared to root-associated microbiome during wheat growth, possibly due to competition with the plant over nitrogen in the root vicinity. We demonstrated functional and phylogenetic division in wheat root zone microbiome in both time and space: pre- and post-spike formation, and root-associated vs. rhizospheric niches. These findings shed light on the dynamics of plant–microbe and microbe–microbe interactions in the developing root zone.
Synthesis of Novel 2-Thiouracil-5-Sulfonamide Derivatives as Potent Inducers of Cell Cycle Arrest and CDK2A Inhibition Supported by Molecular Docking
Authors: Fatahala Samar S. , Sayed Amira I. , Mahgoub Shahenda , Taha Heba , El-Sayed Mohamed-I kotb , El-Shehry Mohamed F. , Awad Samir M. , Abd El-Hameed Rania H.
Published: November 2021

In an effort to discover potent anticancer agents, 2-thiouracil-5-sulfonamides derivatives were designed and synthesized. The cytotoxic activity of all synthesized compounds was investigated against four human cancer cell lines viz A-2780 (ovarian), HT-29 (colon), MCF-7 (breast), and HepG2 (liver). Compounds 6b,d–g, and 7b showed promising anticancer activity and significant inhibition of CDK2A. Moreover, they were all safe when tested on WI38 normal cells with high selectivity index for cancer cells. Flow cytometric analysis for the most active compound 6e displayed induction of cell growth arrest at G1/S phase (A-2780 cells), S phase (HT-29 and MCF-7 cells), and G2/M phase (HepG2 cells) and stimulated the apoptotic death of all cancer cells. Moreover, 6e was able to cause cycle arrest indirectly through enhanced expression of cell cycle inhibitors p21 and p27. Finally, molecular docking of compound 6e endorsed its proper binding to CDK2A, which clarifies its potent anticancer activity.
Gender Differences in Urothelial Bladder Cancer: Effects of Natural Killer Lymphocyte Immunity
Authors: Lutz Charles T. , Livas Lydia , Presnell Steven R. , Sexton Morgan , Wang Peng
Published: November 2021

Men are more likely to develop cancer than women. In fact, male predominance is one of the most consistent cancer epidemiology findings. Additionally, men have a poorer prognosis and an increased risk of secondary malignancies compared to women. These differences have been investigated in order to better understand cancer and to better treat both men and women. In this review, we discuss factors that may cause this gender difference, focusing on urothelial bladder cancer (UBC) pathogenesis. We consider physiological factors that may cause higher male cancer rates, including differences in X chromosome gene expression. We discuss how androgens may promote bladder cancer development directly by stimulating bladder urothelium and indirectly by suppressing immunity. We are particularly interested in the role of natural killer (NK) cells in anti-cancer immunity.
Increased Prevalence of Neuropsychiatric Disorders during COVID-19 Pandemic in People Needing a Non-Deferrable Neurological Evaluation
Authors: Tondo Giacomo , Aprile Davide , Tesser Fabiana , Comi Cristoforo
Published: November 2021

Background: The novel coronavirus disease of 2019 (COVID-19) outbreak provoked a profound healthcare system reorganization. This study aimed to compare the reasons for requesting a non-deferrable neurological evaluation before the COVID-19 pandemic and during the lockdown. Methods: Retrospective observational study including non-deferrable neurological outpatients before the pandemic (pre-COVID-19 group, n = 223) and during the Italian second wave of the COVID-19 pandemic (LOCKDOWN group, n = 318). Results: The number of patients sent for cerebrovascular disorders, headache, and vertigo significantly dropped between the pre-COVID-19 era and the lockdown period. While in the pre-COVID-19 group, the most frequent diagnosis was cerebrovascular disorder; neuropsychiatric disorders ranked first in the LOCKDOWN group. Moreover, the percentage of appropriate non-deferrable neurological evaluations significantly increased in the LOCKDOWN group compared with the pre-COVID-19 group. Discussion: Our study shows a significant increase of neuropsychiatric disorders in non-deferrable neurologic evaluations during the Italian second wave of the COVID-19. Overall, cases were more severe and required a more complex management during the lockdown compared with the pre-COVID era. These findings confirm that a careful approach to prevent the psychological consequences of the pandemic is needed, and long-term rearrangements of the healthcare system are desirable to guarantee appropriate management.
Experimental and Numerical Analysis of Mold Filling in Rotational Molding
Authors: Nieschlag Jonas , Seuffert Julian , Strack Daniel , Friedmann Marco , Kärger Luise , Henning Frank , Fleischer Jürgen
Published: November 2021

This work focuses on the development of a numerical mold filling simulation for the rotational molding process. In the rotational molding process, a dry fiber preform is placed in a mold and impregnated with a thermoset matrix under rotation. Additionally, metallic load introduction elements can be inserted into the mold and joined with co-curing or form-fit, resulting in hybrid drive shafts or tie rods. The numerical model can be used to simulate the impregnation of the preform. Based on the resin transfer molding process, an OpenFOAM solver is extended for the rotational molding process. Permeability, kinetic and curing models are selected and adapted to the materials used. A wireless measurement solution with a capacitive sensor is developed to validate the model. Comparisons between measurements and numerically calculated impregnation times to reach the capacitive sensor with the matrix show good quality of the developed model. The average deviation between calculated result and measured mean values in the experiment is 43.8% the maximum deviation is 65.8% . The model can therefore be used to predict the impregnation progress and the curing state.
Examining the Use of Game-Based Assessments for Hiring Autistic Job Seekers
Authors: Willis Colin , Powell-Rudy Tracy , Colley Kelsie , Prasad Joshua
Published: November 2021

Although people with autism are protected under the Americans with Disabilities Act of 1990, there is little theoretical or practical effort to determine whether traditional pre-employment assessments unfairly impact autistic job seekers. Due to the lack of emphasis on social communication, game-based assessments (GBAs) may offer a way of assessing candidate ability without disadvantaging autistic candidates. A total of 263 autistic job seekers took one of two game-based assessment packages designed to measure cognitive ability. After comparing their results to 323 college-graduate job seekers in the general population, we found that performance on the GBAs was generally similar in both populations, although some small differences were detected. Implications for hiring decisions are discussed.
Evaluating LoRaWAN Connectivity in a Marine Scenario
Authors: Pensieri Sara , Viti Federica , Moser Gabriele , Serpico Sebastiano Bruno , Maggiolo Luca , Pastorino Martina , Solarna David , Cambiaso Andrea , Carraro Carlo , Degano Cristiana , Mainenti Ilaria , Seghezza Silvia , Bozzano Roberto
Published: November 2021

The growing need for interoperability among the different oceanic monitoring systems to deliver services able to answer the requirements of stakeholders and end-users led to the development of a low-cost machine-to-machine communication system able to guarantee data reliability over marine paths. In this framework, an experimental evaluation of the performance of long-range (LoRa) technology in a fully operational marine scenario has been proposed. In-situ tests were carried out exploiting the availability of (i) a passenger vessel and (ii) a research vessel operating in the Ligurian basin (North-Western Mediterranean Sea) both hosting end-nodes, and (iii) gateways positioned on mountains and hills in the inland areas. Packet loss ratio, packet reception rate, received signal strength indicator, signal to noise, and expected signal power ratio were chosen as metrics in line of sight and not the line of sight conditions. The reliability of Long Range Wide Area Network (LoRaWAN) transmission over the sea has been demonstrated up to more than 110 km in a free space scenario and for more than 20 km in a coastal urban environment.
Antifungal Activity and Biocontrol Mechanism of Fusicolla violacea J-1 against Soft Rot in Kiwifruit Caused by Alternaria alternata
Authors: Li Wenzhi , Long Youhua , Mo Feixu , Shu Ran , Yin Xianhui , Wu Xiaomao , Zhang Rongquan , Zhang Zhuzhu , He Linan , Chen Tingting , Chen Jia
Published: November 2021

Alternaria alternata is the main pathogenic species of various crops, including kiwifruit (Actinidia cinensis). In this study, an antagonistic fungus, J-1, with high antifungal activity against A. alternata was isolated from A. cinensis “Hongyang.” The strain J-1 was identified as Fusicolla violacea via morphological identification and DNA sequencing. This study aimed to evaluate the antifungal activity and potential mechanism of the strain J-1 against A. alternata. The strain J-1 exhibited antifungal activity against A. alternata, with an inhibition rate of 66.1% in vitro. Aseptic filtrate (AF) produced by the strain J-1 could suppress the mycelial growth and conidia germination of A. alternata at the inhibition rates of 66.8% and 80%, respectively, as well as suppress the spread of Alternaria rot in fresh kiwifruit. We observed that many clusters of spherical protrusions appeared at the mycelial tips of A. alternata after treatment with 200 mL L−1 AF of J-1. Scanning electron microscopy analysis results showed that the mycelial structures were bent and/or malformed and the surfaces were rough and protuberant. Variations in temperature, pH, and storage time had little effect on the antifungal activity of the AF. Moreover, the AF could damage the integrity of cell membranes and cause intracellular content leakage. Meanwhile, the chitinase and β-1,3-glucanase enzyme activities increased significantly, indicating that the function of A. alternata cell wall was seriously injured. Eleven antimicrobial metabolites were identified by gas chromatography–mass spectrometry (GC–MS). The strain J-I and its AF exhibited well broad-spectrum antifungal activity against Diaporthe eres, Epicoccum sorghinum, Fusarium graminearum, Phomopsis sp., and Botryosphaeria dothidea, with inhibition rates ranging from 34.4% to 75.1% and 42.7% to 75.2%, respectively. Fusicolla violacea J-1 is a potential biocontrol agent against A. alternata and other fungal phytopathogens.
Advances in the Role of Dark Septate Endophytes in the Plant Resistance to Abiotic and Biotic Stresses
Authors: Santos Mila , Cesanelli Ignacio , Diánez Fernando , Sánchez-Montesinos Brenda , Moreno-Gavíra Alejandro
Published: November 2021

Endophytic fungi have been studied in recent decades to understand how they interact with their hosts, the types of relationships they establish, and the potential effects of this interaction. Dark septate endophytes (DSE) are isolated from healthy plants and form melanised structures in the roots, including inter- and intracellular hyphae and microsclerotia, causing low host specificity and covering a wide geographic range. Many studies have revealed beneficial relationships between DSE and their hosts, such as enhanced plant growth, nutrient uptake, and resistance to biotic and abiotic stress. Furthermore, in recent decades, studies have revealed the ability of DSE to mitigate the negative effects of crop diseases, thereby highlighting DSE as potential biocontrol agents of plant diseases (BCAs). Given the importance of these fungi in nature, this article is a review of the role of DSE as BCAs. The findings of increasing numbers of studies on these fungi and their relationships with their plant hosts are also discussed to enable their use as a tool for the integrated management of crop diseases and pests.
The Application Value of Lipoprotein Particle Numbers in the Diagnosis of HBV-Related Hepatocellular Carcinoma with BCLC Stage 0-A
Authors: Zuo Duo , An Haohua , Li Jianhua , Xiao Jiawei , Ren Li
Published: November 2021

Early diagnosis is essential for improving the prognosis and survival of patients with hepatocellular carcinoma (HCC). This study aims to explore the clinical value of lipoprotein subfractions in the diagnosis of hepatitis B virus (HBV)-related HCC. Lipoprotein subfractions were detected by 1H-NMR spectroscopy, and the pattern-recognition method and binary logistic regression were performed to classify distinct serum profiles and construct prediction models for HCC diagnosis. Differentially expressed proteins associated with lipid metabolism were detected by LC-MS/MS, and the potential prognostic significance of the mRNA expression was evaluated by Kaplan–Meier survival analysis. The diagnostic panel constructed from the serum particle number of very-low-density lipoprotein (VLDL), intermediate-density lipoprotein (IDL), and low-density lipoprotein (LDL-1~LDL-6) achieved higher accuracy for the diagnosis of HBV-related HCC and HBV-related benign liver disease (LD) than that constructed from serum alpha-fetoprotein (AFP) alone in the training set (AUC: 0.850 vs. AUC: 0.831) and validation set (AUC: 0.926 vs. AUC: 0.833). Furthermore, the panel achieved good diagnostic performance in distinguishing AFP-negative HCC from AFP-negative LD (AUC: 0.773). We also found that lipoprotein lipase (LPL) transcript levels showed a significant increase in cancerous tissue and that high expression was significantly positively correlated with the poor prognosis of patients. Our research provides new insight for the development of diagnostic biomarkers for HCC, and abnormal lipid metabolism and LPL-mediated abnormal serum lipoprotein metabolism may be important factors in promoting HCC development.
Synthetic Protein Circuits and Devices Based on Reversible Protein-Protein Interactions: An Overview
Authors: Rosa Stefano , Bertaso Chiara , Pesaresi Paolo , Masiero Simona , Tagliani Andrea
Published: November 2021

Protein-protein interactions (PPIs) contribute to regulate many aspects of cell physiology and metabolism. Protein domains involved in PPIs are important building blocks for engineering genetic circuits through synthetic biology. These domains can be obtained from known proteins and rationally engineered to produce orthogonal scaffolds, or computationally designed de novo thanks to recent advances in structural biology and molecular dynamics prediction. Such circuits based on PPIs (or protein circuits) appear of particular interest, as they can directly affect transcriptional outputs, as well as induce behavioral/adaptational changes in cell metabolism, without the need for further protein synthesis. This last example was highlighted in recent works to enable the production of fast-responding circuits which can be exploited for biosensing and diagnostics. Notably, PPIs can also be engineered to develop new drugs able to bind specific intra- and extra-cellular targets. In this review, we summarize recent findings in the field of protein circuit design, with particular focus on the use of peptides as scaffolds to engineer these circuits.
In Vitro Antimicrobial Activity of Medicinal Plant Extracts against Some Bacterial Pathogens Isolated from Raw and Processed Meat
Authors: Meshaal Ahmed Kh. , Hetta Helal F. , Yahia Ramadan , Abualnaja Khamael M. , Mansour Abdallah Tageldein , Al-Kadmy Israa M. S. , Alghamdi Saad , Dablool Anas S. , Emran Talha Bin , Sedky Haitham , Batiha Gaber El-Saber , El-Kazzaz Waleed
Published: November 2021

Background and aim: The poultry meat and its products are considered ideal media for bacterial growth and spoilage, as they are highly nutritive with a favorable pH. The food industry has focused its attention on a great diversity of plant species as food preservatives. The aim of this study was to investigate the presence of Staphylococcus aureus, Escherichia coli O157: H7, and Klebsiella pneumonia in food samples and to evaluate of the antibacterial activity of some medicinal plant extracts against these bacteria. Methods: Raw and processed meat samples (n = 60) were collected from abattoirs and local markets. S. aureus, E. coli O157: H7, and K. pneumonia were isolated, identified by phenotypic methods, and then confirmed by 16S rRNA gene sequencing. The antibacterial activity and spectrum of essential oils and spices powder of cumin, black seeds, cloves, cinnamon, and marjoram was determined against the isolated strains in this study by microbial count and well-diffusion techniques. Results: A total of 33 isolates have been identified as S. aureus, 30 isolates were identified as E. coli O157: H7, and 15 isolates were identified as K. pneumonia. S. aureus, E. coli O157: H7, and K. pneumonia could be detected in both fresh and processed food with higher prevalence in the processed meat. There was a significant decrease in microbial count in treated samples either with the spices powder or essential oils of the tested medicinal plants compared to control samples during storage time period. Furthermore, while the microbial count increased in the control samples, the microbial count decreased to reach zero in almost all treated samples with essential oils after 15 days of storage. Conclusion: S. aureus, E. coli O157: H7, and K. pneumonia are associated with food from animal sources, in either fresh or processed meat samples. The prevalence of them was higher in the processed meat than in fresh meat. The essential oils and spices powder of cumin, black seeds, cloves, cinnamon, and marjoram have an in vitro wide spectrum antibacterial activity with the highest antibacterial activity for the black seeds.
Obtaining and Characterization of New Materials
Authors: Sandu Andrei Victor
Published: November 2021

The main objective of this Special Issue was to publish outstanding papers presenting cutting-edge research in the field of new materials and their understanding [...]
Investigation of the Effect of Pallet Top-Deck Stiffness on Corrugated Box Compression Strength as a Function of Multiple Unit Load Design Variables
Authors: Kim Saewhan , Horvath Laszlo , Russell Jennifer D. , Park Jonghun
Published: November 2021

Unit loads consisting of a pallet, packages, and a product securement system are the dominant way of shipping products across the United States. The most common packaging types used in unit loads are corrugated boxes. Due to the great stresses created during unit load stacking, accurately predicting the compression strength of corrugated boxes is critical to preventing unit load failure. Although many variables affect the compression strength of corrugated boxes, recently, it was found that changing the pallet’s top deck stiffness can significantly affect compression strength. However, there is still a lack of understanding of how these different factors influence this phenomenon. This study investigated the effect of pallet’s top-deck stiffness on corrugated box compression strength as a function of initial top deck thickness, pallet wood species, box size, and board grade. The amount of increase in top deck thickness needed to lower the board grade of corrugated boxes by one level from the initial unit load scenario was determined using PDS™. The benefits of increasing top deck thickness diminish as the initial top deck thickness increases due to less severe pallet deflection from the start. The benefits were more pronounced as higher board grade boxes were initially used, and as smaller-sized boxes were used due to the heavier weights of these unit loads. Therefore, supposing that a company uses lower stiffness pallets or heavy corrugated boxes for their unit loads, this study suggests that they will find more opportunities to optimize their unit loads by increasing their pallet’s top deck thickness.