bluuebunny/crossref_metadata_2025 huggingface dataset:
Pair Bond-Induced Affiliation and Aggression in Male Prairie Voles Elicit Distinct Functional Connectivity in the Social Decision-Making Network
Authors: Gossman Kyle R. , Dykstra Benjamin , García Byron H. , Swopes Arielle P. , Kimbrough Adam , Smith Adam S.
Published: October 2021

Complex social behaviors are governed by a neural network theorized to be the social decision-making network (SDMN). However, this theoretical network is not tested on functional grounds. Here, we assess the organization of regions in the SDMN using c-Fos, to generate functional connectivity models during specific social interactions in a socially monogamous rodent, the prairie voles (Microtus ochrogaster). Male voles displayed robust selective affiliation toward a female partner, while exhibiting increased threatening, vigilant, and physically aggressive behaviors toward novel males and females. These social interactions increased c-Fos levels in eight of the thirteen brain regions of the SDMN. Each social encounter generated a distinct correlation pattern between individual brain regions. Thus, hierarchical clustering was used to characterize interrelated regions with similar c-Fos activity resulting in discrete network modules. Functional connectivity maps were constructed to emulate the network dynamics resulting from each social encounter. Our partner functional connectivity network presents similarities to the theoretical SDMN model, along with connections in the network that have been implicated in partner-directed affiliation. However, both stranger female and male networks exhibited distinct architecture from one another and the SDMN. Further, the stranger-evoked networks demonstrated connections associated with threat, physical aggression, and other aversive behaviors. Together, this indicates that distinct patterns of functional connectivity in the SDMN can be detected during select social encounters.
Case Report: Kawasaki Shock Syndrome With Polycyclic Eruption: A Peculiar Brain Imaging
Authors: Masiello Enrico , Buonsenso Danilo , Lazzareschi Ilaria , Gatto Antonio , Piastra Marco , Chiaretti Antonio , Valentini Piero
Published: October 2021

Kawasaki disease (KD) is a childhood vasculitis of unknown etiology. The present study describes a case of KD shock syndrome that occurred in an infant (age, 16 months) following 7 days of high fever and persistent rash characterized by target-like and purpuric skin lesions. The child developed neurological manifestations such as altered consciousness and irritability. Consequently, brain magnetic resonance imaging (MRI) was performed, revealing an inflammatory involvement of the anterior perforated substance and the hypothalamus. Cerebral involvement on brain MRI is rarely described in KD but when reported is characterized mostly by cerebral vasculitis. We illustrate for the first time in KD an inflammation in the brain not related to vasculitis, reporting peculiar neuroradiological findings. This last aspect has fascinated us in light of recent evidence about the immunological spectrum of Multisystem Inflammatory Syndrome in Children (MIS-C) and Kawasaki-like syndrome in the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) outbreak.
Running on Empty: Of Hypopinealism and Human Seasonality
Authors: Kunz Dieter , Wahnschaffe Amely , Kaempfe Nina , Mahlberg Richard
Published: October 2021

The pineal hormone melatonin is the natural transducer of the environmental light–dark signal to the body. Although the responsiveness to photoperiod is well-conserved in humans, only about 25 percent of the human population experiences seasonal changes in behavior. As a consequence, humans seem to have adapted—at least partly—to the seasonal changes in day length. The aim of the study was to demonstrate that the individual melatonin deficit marker DOC (degree of pineal calcification) is related to variation of seasonal phenomena in humans. Out of 3,011 patients in which cranial computer tomography (cCT) was performed for diagnostic reasons, 97 consecutive “healthy” subjects (43 female, 54 male; age 18–68 yrs, mean ± SD: 35.0 ± 13.1) were included. Exclusion criteria were pathological finding in cCT, acute/chronic illness including alcohol/drug abuse, shift work, and medication, which are known to influence melatonin excretion. The degree of pineal calcification (DOC) was semiquantitatively determined using the previously validated method. The Seasonal Pattern Assessment Questionnaire (SPAQ) was performed in a telephone interview. Twenty-six subjects fulfilled the criteria for seasonal affective disorder (SAD) or subsyndromal (S) SAD. Seasonality was more pronounced in women than in men (SPAQ seasonality score: 7.8 ± 4.0 vs. 4.9 ± 4.5; p = 0.001) and negatively and significantly associated with age (r = −0.178; p = 0.04). The subjective sleep length significantly varied between seasons (one-way repeated measures ANOVA: F = 45.75; p < 0.0001), with sleep during winter being 53 min (±70 min) longer than during summer. Controlling for age, the total seasonality score was negatively and significantly associated with DOC (r94 = −0.214; p = 0.036). Data confirm earlier studies with respect to distribution of seasonality with sex and age. The survival of seasonality in the sleep length of people living in an urban environment underlines functionality of the circadian timing system in modern societies. Moreover, data confirm for the first time that diminished experience of seasonality in behavior is associated with a reduced individual capacity to produce melatonin.
GhCLCg-1, a Vacuolar Chloride Channel, Contributes to Salt Tolerance by Regulating Ion Accumulation in Upland Cotton
Authors: Liu Wei , Feng Junping , Ma Wenyu , Zhou Yang , Ma Zongbin
Published: October 2021

Soil and freshwater salinization is increasingly becoming a problem worldwide and has adversely affected plant growth. However, most of the related studies have focused on sodium ion (Na+) stress, with relatively little research on chloride ion (Cl–) stress. Here, we found that upland cotton (Gossypium hirsutum) plants accumulated Cl– and exhibited strong growth inhibition under NaCl or KCl treatment. Then, a chloride channel gene (GhCLCg-1) was cloned from upland cotton. Phylogenetic and sequence analyses indicated that GhCLCg-1 was highly homologous to AtCLCg and also have conserved voltage_CLC and CBS domains. The subcellular localization assay showed that GhCLCg-1 was localized on the vacuolar membrane. Gene expression analyses revealed that the expression of GhCLCg-1 increased rapidly in cotton in response to chloride stress (NaCl or KCl), and the transcript levels increased as the chloride stress intensified. The overexpression of GhCLCg-1 in Arabidopsis thaliana changed the uptake of ions with a decrease of the Na+/K+ ratios in the roots, stems, and leaves, and enhanced salt tolerance. In contrast, silencing GhCLCg-1 in cotton plants increased the Cl– contents in the roots, stems, and leaves and the Na+/K+ ratios in the stems and leaves, resulting in compromised salt tolerance. These results provide important insights into the toxicity of chloride to plants and also indicate that GhCLCg-1 can positively regulates salt tolerance by adjusting ion accumulation in upland cotton.
“It Is Utterly Out of My Expectation”-A Case Inquiry of Teacher Identity of an EFL Teacher in a Chinese Shadow School Setting
Authors: Wang Feng , Guo Jingwen , Wu Bin , Lin Zhong
Published: October 2021

Though teacher identity is currently receiving an increasing amount of attention in the literature on teacher education and teacher development, little information is available about the richness, fluidity and individuality of EFL teacher identities in various L2 settings. This preliminary case inquiry has echoed that comment by exploring qualitatively how language teacher identity features in the participant's categorization of herself as a professional during her school-to-work transition at a shadow school in hinterland China. Drawing on semi-structured interviews and reflection essays, the triangulated data were analyzed with thematic analysis in order to find categories of enacted identities and key factors that impacted Jane's teacher identity formation. The results indicate four identities existed: an attendant, a firefighter, a coolie and a tramp. The fundamental predicaments hovering over the green employer encompass an array of contextual factors, including (1) overwhelming anxieties for potential early student leavers; (2) overhuge workload and fluid working schedule; (3) endless non-teaching related commitments; (4) lack of career prospect and development ladder. The results of the study contribute to the understanding of the complex and dynamic nature of teacher identity influenced by sociocultural landscapes where language teachers are situated. The research suggests implications for teacher educators and stakeholders on how to transform a novice to a qualified EFL teacher within the ideology and discouraging discourse of a burgeoning privately-owned training market, and on how to mediate green teachers' agency and autonomy against the bottlenecks of their initial years of teaching.
Meager Perception of Preconception Care Among Women Desiring Pregnancy in Rural Areas: A Qualitative Study Using Focus Group Discussions
Authors: Doke Prakash Prabhakarrao , Gothankar Jayashree Sachin , Pore Prasad Dnyandeo , Palkar Sonali Hemant , Chutke Amruta Paresh , Patil Archana Vasantrao , Deshpande Aniruddha Vinayakrao , Bhuyan Khanindra Kumar , Karnataki Madhusudan Vaman , Shrotri Aparna Nishikant
Published: October 2021

Background: India has the second-highest number of under-five deaths in any country in the world. WHO and the Government of India recommended the rollout of preconception care (PCC) to reduce maternal and child mortality. However, very few countries, including India, have started a comprehensive package of PCC services. It implies that women, mainly from rural and tribal areas, are not aware of PCC. PCC has been rolled out through the government health system in two blocks of Nashik district in Maharashtra state, India, among all women who desire to be pregnant within 1 year. This project is the first of its kind in India. To assess basic perceptions, knowledge, and behavior of women on PCC before the implementation of the project, focus group discussions (FGDs) were carried out. The authors think that the finding may help to develop strategies for behavioral change communication.

Methods: From each of the four blocks, two villages having subcenter were selected for conducting FGD. A house-to-house survey was conducted by Accredited Social Health Activist (ASHA) to enlist women who desire a baby in 1 year and invite them to subcenter for FGDs, which were conducted in June 2018.

Results: A total of 76 women having a mean age of 23.97 years participated in the FGDs. Most of them (46.05%) had completed 10 years of education. About 50% of pregnancies were planned. The decision about the timing of the first pregnancy is influenced by the mother-in-law. Women knew that they should not conceive before 20 years of age, and their suboptimal weight may have an adverse impact on the health of the newborn. There are many myths about food like “hot and cold foods” and “forbidden food” etc. Women had some knowledge about the adverse effects of tobacco and alcohol; very few consumed these. Most of them did not practice behaviors or accessed services related to PCC.

Conclusions: Women neither have the knowledge nor adopt behaviors or accessed services related to PCC. Roll out of PCC among them may help in further reduction of maternal and neonatal morbidity and mortality in India.
Internet Public Opinion Diffusion Mechanism in Public Health Emergencies: Based on Entropy Flow Analysis and Dissipative Structure Determination
Authors: Li Wanlian , Zeng Feng , Zhou Wei , Chen Zhishao
Published: October 2021

As an empirical case, this study selected the illegal production process incidents of rabies and DPT (Diphtheria, Pertussis, Tetanus) vaccines by Changchun Longevity Biotechnology Co., Ltd., which occurred in July 2018. Based on the four factors involved in the spread of public opinion, the public health emergency, netizen, network media, and government, Brusselator model, and entropy method were applied to calculate the positive and negative entropy—to verify whether the Internet public opinion system is a dissipative structure. This study verified four evolution mechanisms in Internet public opinion diffusion, among which the trigger point of entropy-control occurred in the germination mechanism, the entropy-controlled disposal point occurred in the outbreak and fluctuating mechanism, and then became latency in the elimination mechanism. It provides a theoretical reference for the government to judge the stage of such diffusion and improve the governance ability of the opinion mentioned above.
Barriers and Facilitators of Physical Activity Participation in Adolescent Girls: A Systematic Review of Systematic Reviews
Authors: Duffey Keeva , Barbosa Ana , Whiting Stephen , Mendes Romeu , Yordi Aguirre Isabel , Tcymbal Antonina , Abu-Omar Karim , Gelius Peter , Breda João
Published: October 2021

Background: Persistent low physical activity (PA) levels among adolescent girls constitute a public health concern that calls for immediate and evidence-based policy action. This systematic review (SR) aimed to summarize evidence from SRs examining the barriers and facilitators of PA participation in adolescent girls. The objectives were to provide a synthesis of the available evidence and identify key areas for fostering gender-responsive action and policy implications.

Methods: A comprehensive search of relevant SR and meta-analyses were performed in PubMed and Cochrane Library, until February 2021. Studies were included if they were SRs or meta-analyses, included adolescent girls aged between 10 and 19 years, and described barriers or facilitators of PA. Two independent authors performed the screening of potentially eligible studies and both assessed the methodological quality of included studies using the AMSTAR 2 tool. The barriers and facilitators were synthesized at environmental, interpersonal, and individual levels.

Results: A total of eight SRs were included in the qualitative synthesis. The most frequent barriers identified were the lack of support from peers, family, and teachers, and the lack of time. The most reported facilitators were weight loss, and support from peers, family, and teachers. Key areas for action and policy implementation include an inclusive approach to curriculum development to address gender norms; adequate training of professionals so they have a range of skills to ensure inclusion of adolescent girls; environmental changes in and out of schools to stimulate participation, to allow adolescent girls to be active in a safe and attractive environment; multistakeholder support at local, regional, and national level in incorporating a gender-responsive approach toward PA participation.

Conclusion: The results highlight a variety of factors that influences the PA participation of adolescent girls. For the attainment of effective policies that increase PA levels in adolescent girls, it is essential to engage several stakeholders at different levels in incorporating a gender-responsive approach toward PA participation.

Systematic Review Registration: PROSPERO, identifier: CRD42020204023.
Case Report: Multimodality Imaging of Infectious Sacroiliitis and Retroperitoneal Abscess Causing Hindlimb Ataxia in a Young Dog
Authors: Jeong Jeongyun , Ko Jaeeun , Kim Jaehwan , Eom Kidong , Cho Youngkwon , Lee Kichang , Yoon Hakyoung
Published: October 2021

A 3-month-old intact male Labrador Retriever was presented for falling trauma and hindlimb ataxia. Radiography indicated radiolucent left sacroiliac joint with irregular margin. Computed tomography revealed thickened sublumbar muscles and hypoattenuated sacroiliac joint while magnetic resonance imaging demonstrated abscess at retroperitoneum and gluteal muscle. Ultrasonography showed lytic left sacroiliac joint with retroperitoneal fluid, and fine needle aspiration resulted Staphylococcus aureus. Hindlimb ataxia was attributed to infectious sacroiliitis and its secondary retroperitoneal abscess. As far as the authors' knowledge, this is the first report of multimodality imaging of infectious sacroiliitis with retroperitoneal abscess caused by S. aureus in a dog.
Improving the Robustness of AI-Based Malware Detection Using Adversarial Machine Learning
Authors: Patil Shruti , Varadarajan Vijayakumar , Walimbe Devika , Gulechha Siddharth , Shenoy Sushant , Raina Aditya , Kotecha Ketan
Published: October 2021

Cyber security is used to protect and safeguard computers and various networks from ill-intended digital threats and attacks. It is getting more difficult in the information age due to the explosion of data and technology. There is a drastic rise in the new types of attacks where the conventional signature-based systems cannot keep up with these attacks. Machine learning seems to be a solution to solve many problems, including problems in cyber security. It is proven to be a very useful tool in the evolution of malware detection systems. However, the security of AI-based malware detection models is fragile. With advancements in machine learning, attackers have found a way to work around such detection systems using an adversarial attack technique. Such attacks are targeted at the data level, at classifier models, and during the testing phase. These attacks tend to cause the classifier to misclassify the given input, which can be very harmful in real-time AI-based malware detection. This paper proposes a framework for generating the adversarial malware images and retraining the classification models to improve malware detection robustness. Different classification models were implemented for malware detection, and attacks were established using adversarial images to analyze the model’s behavior. The robustness of the models was improved by means of adversarial training, and better attack resistance is observed.
Investigation of Hysteresis Effect in Torque Performance for a Magnetorheological Brake in Adaptive Knee Orthosis
Authors: Shiao Yaojung , Gadde Premkumar
Published: October 2021

Semi-active knee orthosis (SAKO) is a kind of wearable lower-limb exoskeleton that uses actuators to support the regular biomechanical functions. It is much better than conventional knee orthosis (CKO) devices because of its high torque to volume ratio (TVR) and low mass. Magnetorheological (MR) brake is one of the smart actuators that can be used as an active resistance device in SAKO. It has advantages of fast response, low power consumption, and low vibration operation. This smart brake also has wide applications in the robotic and automotive industries. However, the electromagnetic setup in MR brakes has a hysteresis problem. This paper aims to turn this hysteresis problem into an advantage to save the power consumption of MR brake. Since the SAKO needs precise torque control, this research studied the hysteresis effect on the torque performance of MR brake. A less energy-consuming PWM actuation signal is proposed to activate the MR brake. The effects of frequency and duty cycle of PWM actuation signal on MR brake performance are also investigated. The electromagnetic (EM) and mechanical models of the MR brake were developed to simulate performance. Initial validation of these models is done by simulating the MR brake model with the DC actuation signal in finite element analysis software. For the final validation, the model simulation results are compared with experimental results. The factors affecting the steady torque and the response time of the MR brake are studied to find the optimal frequency and duty cycle for the applied PWM signal. This study revealed that the proposed new PWM actuation signal with a 5 kHz frequency and 60% duty cycle can power the MR brake to maintain steady torque. By turning hysteresis into an advantage, it saves 40% power consumption of MR brake compared to DC signal.
Large Stepwise Discrete Microsystem Displacements Based on Electrostatic Bending Plate Actuation
Authors: Schmitt Lisa , Hoffmann Martin
Published: October 2021

We present the design, fabrication, and experimental characterization of microsystems achieving large and stepwise discrete displacements. The systems consist of electrostatic bending plate actuators linked in a chain with increasing electrode gaps to allow a stepwise system displacement. A derived analytic transfer function permits to evaluate the influence of the system components on both the total and the stepwise system displacement. Based on calculation and simulation results, systems featuring 5, 8, 10, 13, and 16 steps are modeled and fabricated using a dicing-free SOI-fabrication process. During experimental voltage- and time-dependent system characterization, the minimum switching speed of the electrostatic actuators is 1 ms. Based on the guiding spring stiffness and the switching time, step-by-step and collective activations of the microsystems are performed and the system properties are derived. Furthermore, we analyze the influence of the number of steps on the total system displacement and present 16-step systems with a total maximum displacement of 230.7 ± 0.9 µm at 54 V.
Know the Farmer That Feeds You: A Cross-Country Analysis of Spatial-Relational Proximities and the Attractiveness of Community Supported Agriculture
Authors: Gugerell Christina , Sato Takeshi , Hvitsand Christine , Toriyama Daichi , Suzuki Nobuhiro , Penker Marianne
Published: October 2021

While food production and consumption processes worldwide are characterized by geographical and social distance, alternative food networks aim to reconnect producers and consumers. Our study proposes a framework to distinguish multiple dimensions of proximity in the context of Community Supported Agriculture (a type of alternative food network) and to quantitatively evaluate them. In a principal component analysis, we aggregated various detailed proximity items from a multinational survey using principal component analysis and examined their relationship with the attractiveness of Community Supported Agriculture in a multiple regression analysis. Our findings highlight the importance of relational proximity and thus of increasing trust, collaboration, and the sharing of values and knowledge within and across organizations in the food system. Rather than focusing on spatial proximity, increasing relational proximity might support alternative food networks, such as Community Supported Agriculture.
Qualitative Cost-Benefit Analysis of Using Pesticidal Plants in Smallholder Crop Protection
Authors: Mkindi Angela G. , Coe Richard , Stevenson Philip C. , Ndakidemi Patrick A. , Belmain Steven R.
Published: October 2021

Assessing the potential drivers of farmers using pesticidal plants for crop protection is essential for wider adoption. However, few studies have focused on collaborative assessments of the underlying trade-offs when using pesticidal plant extracts for pest control. Smallholder farmers in northern Tanzania involved in farmer driven research assessing pesticidal plants evaluated the costs, benefits, trade-offs and areas for future investment. A questionnaire was used to collect demographic information from 77 farmers and their views on pest problems and crop protection in common bean production. This was followed by small focus group discussions (n = 9) using a participatory framework to elucidate the costs and benefits of adopting pesticidal plant technology. A multiple correspondence analysis showed that pesticidal plant use was associated with men greater than 50 years old, and synthetic pesticide use was associated with younger aged farmers and women. Farmers who used synthetics generally did not report the presence of common pest species found in common bean production, whereas farmers who used pesticidal plants were associated with more frequent reports of pest species. This participatory cost–benefit analysis highlighted that tools and processing challenges were the main costs to using pesticidal plants. The main benefit reported when using pesticidal plants was a general improvement to family health. Farmers expressed overall a positive outcome when using pesticidal plants for crop protection and recommended that future investments focus on improving access to tools and education regarding plant processing and extraction to improve uptake of the technology by smallholder farmers.
Combined Use of Calciprill and Sodium Silicate Improves Chemical Properties of Low-pH Soil
Authors: Ng Ji Feng , Ahmed Osumanu Haruna , Omar Latifah , Jalloh Mohamadu Boyie , Kwan Yee Min , Poong Ken Heong , Musah Adiza Alhassan
Published: October 2021

The highly weathered, acidic tropical soils are generally less fertile because of their low pH, high exchangeable acidity, and Al, Fe, and Mn saturations. Using soil amendments to solve the aforementioned problems is essential. To this end, Calciprill and sodium silicate are worth considering because of their high neutralizing value and dissolution to suppress exchangeable acidity and Al, Fe, and Mn hydrolysis, while at the same time increasing soil pH to improve the availability of inorganic N, available P, exchangeable base cations, and Effective Cation Exchange Capacity (ECEC). An incubation study was conducted to determine the right combination of Calciprill and sodium silicate to reduce exchangeable acidity and Al and Fe hydrolysis to improve inorganic N, available P, exchangeable base cations availability, and ECEC. Bekenu series (Typic Paleudults) was incubated with a combined use of Calciprill at 80%, 90%, and 100% Ca saturations and sodium silicate at 90, 105, 120, 135, and 150 kg ha−1 for 40, 80, and 120 days, respectively. The laboratory incubation study was carried out using a Completely Randomized Design (CRD) with triplicates to determine the aforementioned treatment effects of the combined use of Calciprill and sodium silicate on soil pH, exchangeable, Al, inorganic N, available P, electrical conductivity, exchangeable cations (K, Ca, Mg, Na, Fe, and Mn), and ECEC using standard procedures. Soils with both amendments significantly increased soil inorganic N, available P, electrical conductivity, exchangeable base cations (K, Ca, Mg, and Na), and ECEC. This was because the amendments increased soil pH by suppressing exchangeable acidity. Moreover, they transformed Al, Fe, and Mn ions into insoluble compounds such as Al and Fe hydroxides and Mn oxides because of their high inherent exchangeable cations, especially Ca and Na. This suggests that the combined use of Calciprill and sodium silicate can enhance soil productivity. The most suitable combination is 7.80 g Calciprill and 9.26 g sodium silicate (C3S5) per one kilogram soil. Additionally, the findings provide fundamental information for future greenhouse and field trials to determine the effects of the suitable combination of the amendments uncovered by this present study on soil health and crop productivity.
Assessing and Modeling Ecosystem Carbon Exchange and Water Vapor Flux of a Pasture Ecosystem in the Temperate Climate-Transition Zone
Authors: Li Zhou , Chen Chao , Nevins Andrew , Pirtle Todd , Cui Song
Published: October 2021

The rising frequency of extreme weather events and global warming are greatly challenging pastoral ecosystem productivity, particularly in the temperate climate-transition regions. While this could cause greater gross primary production (GPP) mainly contributed by the warm-season vegetation, the consequences for the dynamics of net ecosystem exchange (NEE) and hydrological responses (e.g., evapotranspiration, ET) on an ecosystem level are poorly known. Here, we investigated the evolution of plant phenology, nutritive value, energy balance, and carbon/water budgets of a cool-season dominated pastoral ecosystem in the temperate zone; integrating both eddy covariance (EC) flux measurement and simulation modeling-based uncertainty analysis. Throughout the two-year duration (2017–2018) of this study, the entire pasture ecosystem remained a strong carbon sink (NEE = −1.23 and −1.95 kg C m−2, respectively) with 74% and 62% of available energy loss explained by EC fluxes, respectively. The cumulative ET was 735.8 and 796.8 mm, respectively; and the overall ecosystem water use efficiency (EWUE) were calculated as 6.5 g C kg−1 water across both growing seasons. The above-ground biomass yield agreed with the cumulative GPP and was inversely correlated with grass nutritive value. The uncertainty analysis indicated that accurate EC flux gap-filling models could be constructed using support vector machine trained time-series models (NEE, R2 = 0.77, RMSE = 11.8; ET, R2 = 0.90, RMSE = 73.8). The performance benchmarking tests indicated that REddyProc-based gap-filling performance was very limiting and highly variable (NEE, R2 = 0.21–0.64; ET, R2 = 0.79–0.87), particularly for estimating NEE. Overall, the warm-season vegetation encroachment greatly filled the production gap of cool-season grasses, leading to greater cumulative NEE and EWUE on a system level, compared with those from many other reported field-crop or grassland studies using EC approaches. The complex and dynamic nature of grassland ecosystems greatly challenged the conventional REddyProc-based EC flux gap-filling performance. However, accurate machine learning models could be constructed for error/uncertainty control purposes and, thus, should be encouraged in future studies.
Predicting the Emergence of Echinochloa crus-galli (L.) P. Beauv. in Maize Crop in Croatia with Hydrothermal Model
Authors: Šoštarčić Valentina , Masin Roberta , Loddo Donato , Svečnjak Zlatko , Rubinić Vedran , Šćepanović Maja
Published: October 2021

Echinochloa crus-galli (L.) P. Beauv. is the most common monocotyledonous weed in maize crops in Croatia. Crop–weed interference is influenced by weed emergence patterns, and knowledge of the timing of weed emergence is crucial for the development of an efficient integrated weed-management program. Therefore, two-year field experiments were conducted in a maize crop sown in early May in continental Croatia to determine the emergence pattern of E. crus-galli from natural seedbank. In laboratory studies, the estimated base temperature and base water potential for the Croatian ecotype of E. crus-galli were 10.8 °C and −0.97 MPa, respectively. Then, the estimated germination parameters were compared with the values embedded in the AlertInf model from Italy (Veneto) to calibrate this hydrothermal model. The estimated hydrothermal units were around 28 for the onset (10%) and 93 for the middle (50%) emergence of E. crus-galli. Our findings showed that the AlertInf model satisfactorily simulated the emergence of E. crus-galli in maize crop in Croatia (EF = 0.97 in 2019 and 0.98 in 2020), indicating its potential use in other geographical areas
Impact of Cefazolin Shortage on Clinical Outcomes of Adult Patients with Bacteremia Caused by Methicillin-Susceptible Staphylococcus aureus in a Tertiary Care University Hospital
Authors: Uda Atsushi , Onuma Kenichiro , Shigemura Katsumi , Kitagawa Koichi , Yan Yonmin , Osawa Kayo , Yano Ikuko , Miyara Takayuki
Published: October 2021

Cefazolin is an essential antibiotic used for treating bacteremia; in particular, it is recommended as a first-line agent for infections caused by methicillin-susceptible Staphylococcusaureus (MSSA). In March 2019, problems with a major antibiotic supplier caused a critical shortage of cefazolin in Japan; however, the impact of the cefazolin shortage on clinical outcomes remains unknown. This study aimed to evaluate the effect of the cefazolin shortage in patients with MSSA bacteremia. Data from 75 patients were compared between the pre-shortage (March 2018–January 2019, n = 39) and post-shortage (March 2019–January 2020, n = 36) periods. There were no significant differences in the demographic characteristics between the two groups, and the cefazolin shortage did not worsen clinical outcomes such as adverse drug reactions, treatment failure, and 30-day mortality. In the post-shortage group, ampicillin/sulbactam and benzylpenicillin were more frequently administered as alternative antibiotics for empirical and definitive therapy (10% vs. 31%, p = 0.042; 0% vs. 19%, p = 0.004, respectively). Multivariate analysis revealed that the broad-spectrum antibiotics for definitive therapy, such as antipseudomonal penicillin, were associated with treatment failure in patients with MSSA bacteremia (OR = 17, p = 0.003). Hence, narrow-spectrum antibiotics should be prescribed for MSSA bacteremia as alternatives during a cefazolin shortage.
Outpatient Antibiotic Prescribing Patterns and Appropriateness for Children in Primary Healthcare Settings in Beijing City, China, 2017–2019
Authors: Wushouer Haishaerjiang , Du Kexin , Chen Shicai , Zhou Yue , Zheng Bo , Guan Xiaodong , Shi Luwen
Published: October 2021

(1) Background: Few studies have focused on antibiotic use and appropriateness in children in primary health institutions (PHIs). This study aimed to identify the patterns and appropriateness of antibiotic use for children in PHIs in Beijing, China. (2) Methods: Outpatient prescriptions of 327 PHIs from 2017 to 2019 for patients <18 years old were collected. Prescriptions were described using quantity indicators. Antibiotics were categorized according to ATC classification J01 and Access, Watch, Reserve grouping. Appropriateness was reviewed by experts using three subtypes of irrational prescriptions (irregular, inappropriate, and abnormal). (3) Results: 20,618 prescriptions were collected in total. The antibiotic prescription rate (APR) was 15.1% (N = 3113). Among antibiotic prescriptions, J01FA Macrolides were the most used (N = 1068, 34.9%). The Watch group constituted 89.0% (N = 2818) of total antibiotic use. Bronchitis (N = 1059, 35.2%) was the most common diagnosis. A total of 292 instances of irrational antibiotic use were identified, with inappropriate prescriptions being the most prevalent subtype (N = 233, 79.8%). (4) Conclusion: Although APR for children in PHIs in Beijing was relatively low, the pattern of antibiotic use differed from other countries. Further studies are needed to optimize antibiotic use for children in PHIs under different levels of economic development.
National Antibiotics Utilization Trends for Human Use in Tanzania from 2010 to 2016 Inferred from Tanzania Medicines and Medical Devices Authority Importation Data
Authors: Sangeda Raphael Zozimus , Saburi Habibu Ally , Masatu Faustine Cassian , Aiko Beatrice Godwin , Mboya Erick Alexander , Mkumbwa Sonia , Bitegeko Adonis , Mwalwisi Yonah Hebron , Nkiligi Emmanuel Alphonse , Chambuso Mhina , Sillo Hiiti Baran , Fimbo Adam M. , Horumpende Pius Gerald
Published: October 2021

Antimicrobial use (AMU) is one of the major drivers of emerging antimicrobial resistance (AMR). The surveillance of AMU, which is a pillar of AMR stewardship (AMS), helps devise strategies to mitigate AMR. This descriptive, longitudinal retrospective study quantified the trends in human antibiotics utilization between 2010 and 2016 using data on all antibiotics imported for systemic human use into Tanzania’s mainland. Regression and time series analyses were used to establish trends in antibiotics use. A total of 12,073 records for antibiotics were retrieved, totaling 154.51 Defined Daily Doses per 1000 inhabitants per day (DID), with a mean (±standard deviation) of 22.07 (±48.85) DID. The private sector contributed 93.76% of utilized antibiotics. The top-ranking antibiotics were amoxicillin, metronidazole, tetracycline, ciprofloxacin, and cefalexin. The DIDs and percentage contribution of these antibiotics were 53.78 (34.81%), 23.86 (15.44), 20.53 (13.29), 9.27 (6.0) and 6.94 (4.49), respectively. The time series model predicted a significant increase in utilization (p-value = 0.002). The model forecasted that by 2022, the total antibiotics consumed would be 89.6 DIDs, which is a 13-fold increase compared to 2010. Government intervention to curb inappropriate antibiotics utilization and mitigate the rising threat of antibiotic resistance should focus on implementing AMS programs in pharmacies and hospitals in Tanzania.
Extended-Spectrum-β-Lactamase- and AmpC-Producing Escherichia coli in Domestic Dogs: Spread, Characterisation and Associated Risk Factors
Authors: Formenti Nicoletta , Grassi Andrea , Parisio Giovanni , Romeo Claudia , Guarneri Flavia , Birbes Laura , Pitozzi Alessandra , Scali Federico , Maisano Antonio Marco , Boniotti Maria Beatrice , Pasquali Paolo , Alborali Giovanni Loris
Published: October 2021

In veterinary medicine, the issue of antimicrobial resistance was mainly addressed in food-producing animals (although companion animals also deserve attention). Indeed, these species may be reservoir of resistant microorganisms, such as extended-spectrum β-lactamase and AmpC (ESBL/AmpC)-producing bacteria. Dogs in particular may transmit them to close-contact humans. Overall 266 faecal samples of healthy dogs were microbiologically and molecularly analyzed to investigate ESBL/AmpC-producing Escherichia coli and the effects of host and environmental factors on their spread. A prevalence of 25.9% of ESBL/AmpC-producing E. coli, supported by blaCTX-M (79.7%), blaTEM (47.8%), blaCMY (13%), and blaSHV (5.8%) gene detection, emerged. Dogs frequenting extra-urban environments showed significantly higher odds of being positive to ESBL/AmpC E. coli (30.2%) compared to urban dogs (16.7%) identifying the environment as a risk factor. About 88.4% of isolates were resistant to cephalosporins, 8.7% to cephalosporins and carbapenems, and 2.9% to cephalosporins, carbapenems, and penicillins. ESBL/AmpC-producing E. coli expressing blaCMY were significantly more resistant to cefoxitin, cefotaxime/clavulanic acid and ceftazidime/clavulanic acid, highlighting its negative effects. Our results suggest the role of domestic dogs as a maintenance host of ESBL/AmpC-producing E. coli leading to a constant health monitoring. The recorded resistances to carbapenems implies attention and further investigations.
Bacillus amyloliquefaciens—Derived Lipopeptide Biosurfactants Inhibit Biofilm Formation and Expression of Biofilm-Related Genes of Staphylococcus aureus
Authors: Englerová Karolína , Bedlovičová Zdenka , Nemcová Radomíra , Király Ján , Maďar Marián , Hajdučková Vanda , Styková Eva , Mucha Rastislav , Reiffová Katarína
Published: October 2021

Biosurfactants (BSs) are surface-active compounds produced by diverse microorganisms, including the genus Bacillus. These bioactive compounds possess biological activities such as antiadhesive, antimicrobial and antibiofilm effects that can lead to important applications in combating many infections. Based on these findings, we decided to investigate the antibiofilm activity of BSs from the marine Bacillus amyloliquefaciens against Staphylococcus aureus CCM 4223. Expression of biofilm-related genes was also evaluated using qRT-PCR. Isolated and partially purified BSs were identified and characterized by molecular tools and by UHPLC-DAD and MALDI-TOF/MS. Bacillus amyloliquefaciens 3/22, that exhibited surfactant activity evaluated by oil spreading assay, was characterized using the 16S rRNA sequencing method. Screening by PCR detected the presence of the sfp, srfAA, fenD and ituD genes, suggesting production of the lipopeptides (LPs) surfactin, fengycin and iturin. The above findings were further supported by the results of UHPLC-DAD and MALDI-TOF/MS. As quantified by the crystal violet method, the LPs significantly (p < 0.001) reduced biofilm formation of S. aureus in a dose-dependent manner and decreased expression of biofilm-related genes fnbA, fnbB, sortaseA and icaADBC operon. Data from our investigation indicate a promising therapeutic application for LPs isolated from B. amyloliquefaciens toward prevention of S. aureus biofilm infections.
Hydroxytyrosol-Fortified Foods Obtained by Supercritical Fluid Extraction of Olive Oil
Authors: Bartella Lucia , Mazzotti Fabio , Talarico Ines Rosita , Santoro Ilaria , Di Donna Leonardo
Published: October 2021

A new original process has been set-up to obtain hydroxytyrosol-enriched food by means of CO2 supercritical fluid extraction. The process is based on the direct adsorption of hydroxytyrosol on selected foodstuff (e.g., flour, whole-wheat flour, and sugar) when placed in contact with olive oil (which is known to contain this phenol) under controlled temperature and pressure conditions of supercritical CO2. The transfer of the nutraceutical to the foodstuff was initially evaluated using a fortified olive oil model and was then applied to commercial olive oil and foodstuff. The yield of the hydroxytyrosol transfer was demonstrated to be quantitative. In order to prove the suitability of the final products, hydroxytyrosol-enriched flour was used to prepare a bread roll, which maintained the nutraceutical characteristics after the cooking. Finally, DPPH based experiments were performed to prove the radical scavenging activity of the functionalized foodstuff.
Influence of Redox Imbalances on the Transposition of Insertion Sequences in Deinococcus geothermalis
Authors: Ye Qianying , Lee Chanjae , Shin Eunjung , Lee Sung-Jae
Published: October 2021

The transposition of insertion sequence elements was evaluated among different Deinococcus geothermalis lineages, including the wild-type, a cystine importer-disrupted mutant, a complemented strain, and a cystine importer-overexpressed strain. Cellular growth reached early exponential growth at OD600 2.0 and late exponential growth at OD600 4.0. Exposing the cells to hydrogen peroxide (80–100 mM) resulted in the transposition of insertion sequences (ISs) in genes associated with the carotenoid biosynthesis pathway. Particularly, ISDge7 (an IS5 family member) and ISDge5 (an IS701 family member) from the cystine importer-disrupted mutant were transposed into phytoene desaturase (dgeo_0524) via replicative transposition. Further, the cystine importer-overexpressed strain Δdgeo_1985R showed transposition of both ISDge2 and ISDge5 elements. In contrast, IS transposition was not detected in the complementary strain. Interestingly, a cystine importer-overexpressing strain exhibited streptomycin resistance, indicating that point mutation occurred in the rpsL (dgeo_1873) gene encoding ribosomal protein S12. qRT-PCR analyses were then conducted to evaluate the expression of oxidative stress response genes, IS elements, and low-molecular-weight thiol compounds such as mycothiol and bacillithiol. Nevertheless, the mechanisms that trigger IS transposition in redox imbalance conditions remain unclear. Here, we report that the active transposition of different IS elements was affected by intracellular redox imbalances caused by cystine importer deficiencies or overexpression.
Taxifolin as a Promising Ingredient of Cosmetics for Adult Skin
Authors: Micek Iwona , Nawrot Joanna , Seraszek-Jaros Agnieszka , Jenerowicz Dorota , Schroeder Grzegorz , Spiżewski Tomasz , Suchan Adela , Pawlaczyk Mariola , Gornowicz-Porowska Justyna
Published: October 2021

Active substances, effective in the reduction in or delay of skin changes caused by aging occurring in natural compounds, are desirable. Taxifolin (TXF), a flavonoid of strong antioxidant activity found in the plant Stizolophus balsamita (S. balsamita), has been tested for its biological effects on adult human skin. The aim of the study was to investigate the effects of two creams: 3% S. balsamita extract and 3% TXF on the function of adult skin. In total, 97 Caucasian women with clinical signs of skin aging were investigated. The biophysical and biomechanical skin parameters were measured before and after applying the creams, using Colorimeter CL400, Mexameter MX16, Skin-pH-Meter PH900, Skin-Thermometer ST 500, Glossymeter GL200, and Cutiscan SC100. Patch tests were performed with the investigated products to assess their potential irritant properties. The percutaneous penetration of creams was examined with the use of electrospray ionization mass spectrometry (ESI-MS) and confocal Raman spectroscopy. The 3% S. balsamita extract cream reduced hyperpigmentation, erythema, and elevated pH. All the tested preparations were proven to be nonirritant. A higher penetration rate was revealed for the 3% TXF cream than for the 3% S. balsamita extract cream. A total of 3% TXF cream improved skin viscoelasticity. The obtained results suggested that S. balsamita extract and TXF may be considered as ingredients of skincare products for adults.
Cimicifugae Rhizoma Extract Attenuates Oxidative Stress and Airway Inflammation via the Upregulation of Nrf2/HO-1/NQO1 and Downregulation of NF-κB Phosphorylation in Ovalbumin-Induced Asthma
Authors: Lim Je-Oh , Song Kwang Hoon , Lee Ik Soo , Lee Se-Jin , Kim Woong-Il , Pak So-Won , Shin In-Sik , Kim Taesoo
Published: October 2021

Cimicifugae Rhizoma has been used as a medicinal herb for fever, pain, and inflammation in East Asia. We conducted this study because the effect of Cimicifugae Rhizoma extract (CRE) on allergic asthma has not yet been evaluated. To induce allergic airway inflammation, we intraperitoneally injected ovalbumin (OVA) mixed with aluminum hydroxide into mice twice at intervals of 2 weeks (Days 0 and 14) and then inhaled them thrice with 1% OVA solution using a nebulizer (Days 21 to 23). CRE (30 and 100 mg/kg) was administered orally daily for 6 days (Days 18 to 23). The mice showed remarkable reduction in allergic inflammation at 100 mg/kg of CRE, as evidenced by decreased inflammatory cell counts, pro-inflammatory cytokine levels, OVA-specific immunoglobulin E level, airway hyperresponsiveness, and production of mucus. Additionally, these effects were involved with the enhancement of heme oxygenase-1 (HO-1), NAD(P)H: quinone oxidoreductase (NQO1), and nuclear factor erythroid 2-related factor 2 (Nrf2) expression and reduction of nuclear factor-κB (NF-κB) phosphorylation and matrix metalloproteinase-9 expression. Our findings indicated that CRE effectively protected against OVA-induced inflammation and oxidative stress via upregulation of the Nrf2/HO-1/NQO1 signaling and downregulation of NF-κB phosphorylation in asthma caused by OVA.
Musical Mix Clarity Prediction Using Decomposition and Perceptual Masking Thresholds
Authors: Parker Andrew , Fenton Steven
Published: October 2021

Objective measurement of perceptually motivated music attributes has application in both target-driven mixing and mastering methodologies and music information retrieval. This work proposes a perceptual model of mix clarity which decomposes a mixed input signal into transient, steady-state, and residual components. Masking thresholds are calculated for each component and their relative relationship is used to determine an overall masking score as the model’s output. Three variants of the model were tested against subjective mix clarity scores gathered from a controlled listening test. The best performing variant achieved a Spearman’s rank correlation of rho = 0.8382 (p < 0.01). Furthermore, the model output was analysed using an independent dataset generated by progressively applying degradation effects to the test stimuli. Analysis of the model suggested a close relationship between the proposed model and the subjective mix clarity scores particularly when masking was measured using linearly spaced analysis bands. Moreover, the presence of noise-like residual signals was shown to have a negative effect on the perceived mix clarity.