bluuebunny/crossref_metadata_2025 huggingface dataset:
Isolation and Evaluation of Indigenous Isolates of Beauveria bassiana and Synergistic Control of Spodoptera frugiperda with the Parasitoid Microplitis prodeniae
Authors: Zhang Ling-Wei , Lu Fang-Fang , Zhu Lu , Zhou Chen-Xu , Xu Xiao-Miao , Zhang Nan , Zhou Li-Jun , Desneux Nicolas , Wang Yao-Hui , Dong Yong-Cheng
Published: November 2024

Entomopathogenic fungi (EPFs) are capable of infecting a variety of insect pests and are widely used as biopesticides worldwide. This study intended to isolate indigenous EPFs from cadavers of Protaetia brevitarsis and investigate their effects on the fall armyworm Spodoptera frugiperda (FAW), a globally widespread invasive pest. Morphological and molecular analyses confirmed four native EPF strains all belong to Beauveria bassiana. Pathogenicity tests showed they were virulent toward FAW 1st instar larvae. The application of EPFs either by dipping or spraying significantly increased the larval mortalities compared to the control group, with corrected mortalities ranging from 92% to 73% after dipping in a fungal suspension of 108 conidia/mL, and those ranging from 76% to 35% after spraying with a fungal suspension of 107 conidia/mL. Our findings revealed the infectivity of four strains to FAW larvae significantly changed in a dose- and time-dependent manner. In addition, the combination use of the local B. bassiana strain and parasitoid Microplitis prodeniae resulted in a significantly enhanced S. frugiperda 3rd instar larval mortality compared to a single inoculation with one of them, suggesting an apparent synergistic effect for the joint application of these two biological control agents. The mortality inflicted by B. bassiana was probably promoted by the release of parasitoids, since the parasitoids’ movements and attacks could strengthen the fungal distribution and infection processes. This study underscores the potential of the combination use of EPFs and parasitoids against S. frugiperda early-instar larvae, and provides insights into the consequences of integrating these EPFs into integrated pest management systems.
Information and Analytical System Monitoring and Assessment of the Water Bodies State in the Mineral Resources Complex
Authors: Afanaseva Olga , Afanasyev Mikhail , Neyrus Semyon , Pervukhin Dmitry , Tukeev Dmitry
Published: November 2024

Currently, one of the most pressing global issues is ensuring that human activities have access to water resources that meet essential quality standards. This challenge is addressed by implementing a series of organizational and technical measures aimed at preserving the ecology of water basins and reducing the level of harmful industrial emissions and other pollutants in the aquatic environment. To guarantee the necessary quality of water resources, monitoring is conducted based on selected parameters using various methods and means of technical quality control. From these results, suitable measures are formulated and applied to maintain water quality. Various scientific works extensively discuss different approaches to water quality management and compliance with specified requirements. Modern strategies for developing water monitoring systems leverage the capabilities of information systems that collect, process, store, and transmit information, enabling the resolution of issues in geographically distributed water bodies in real time. This paper proposes an approach that employs mathematical methods to identify the most significant factors determining water quality and to assess their interrelations using methods of a priori ranking, multivariate correlation regression analysis, and integral quantitative assessment. A hardware and software solution for the development of a unified integrated information and analytical system is proposed. This system enables continuous monitoring and assessment of water bodies based on a set of key parameters, addressing a range of critical tasks. This paper provides a detailed description of the software product, presents a demonstration using real-world data, and discusses the anticipated benefits of implementing such an information and analytical system.
A Very Low-Calorie Ketogenic Diet Approach for Post-Bariatric Weight Regain: A Pilot Study
Authors: Ernesti Ilaria , Watanabe Mikiko , Genco Alfredo
Published: November 2024

Weight regain (WR) after bariatric surgery, particularly sleeve gastrectomy, is a significant challenge, often driven by a combination of metabolic, behavioral, and lifestyle factors. Non-surgical interventions to manage WR are critical, given the increased risks and reduced efficacy of revisional surgeries. In this context, very low-calorie ketogenic diets (VLCKDs) have gained attention for their potential to promote weight loss and improve body composition in individuals struggling with WR. This study assessed the safety and efficacy of a VLCKD in 11 patients who experienced WR following sleeve gastrectomy. Over an 8-week period, patients demonstrated a significant average weight loss of 6.3% (p = 0.005), along with improvements in body composition, including reductions in body fat percentage (p = 0.003) and waist circumference (p = 0.003). Metabolic markers, such as insulin resistance (HOMA-IR), also improved significantly (p = 0.041). Although a decrease in the glomerular filtration rate was observed (p = 0.007), this finding is unlikely to be clinically relevant over the short term. Importantly, no major adverse events were reported, with only mild constipation observed. These results suggest that VLCKDs may be a promising non-surgical approach for managing WR post-bariatric surgery, though further studies are needed to assess long-term effects, especially on renal function.
Direct Oral Anticoagulants Versus Aspirin for Stroke Prevention After Embolic Stroke of Undetermined Source: An Updated Meta-Analysis of Randomized Controlled Trials
Authors: Pirera Edoardo , D’Anna Lucio , Di Raimondo Domenico , Tuttolomondo Antonino
Published: November 2024

Background: Four randomized controlled trials (RCTs) did not show a benefit of direct oral anticoagulant (DOAC) treatment compared with antiplatelet therapy for the prevention of recurrent strokes in patients with embolic stroke of undetermined source (ESUS). However, the balance between efficacy and safety in subgroups needs to be better defined. We aimed to assess the relative benefits of DOACs in key subgroups of adult patients with ESUS. Methods: We searched major databases (PubMed, Embase, CENTRAL, and Web of Science) for RCTs published from inception to 16 June 2024. The primary outcome was recurrent stroke, and the main safety outcomes were major bleeding and clinically relevant non-major bleeding (CRNB). We assessed the risk of bias using the Cochrane Risk of Bias tool 2. Results: We identified four RCTs, involving a total of 13,970 patients with ESUS. Compared to antiplatelet therapy, treatment with DOAC did not reduce the risk of recurrent stroke (RR 0.95, 95% CI 0.83–1.08, p = 0.45) or ischemic stroke (RR 0.92, 95% CI 0.80–1.05, p = 0.22) or increase major bleeding (RR 1.57, 95% CI 0.87–2.83; p = 0.14). DOAC treatment was associated with a significantly higher risk of CRNMB compared to aspirin (RR 1.52, 95% CI: 1.22–1.90; p = 0.0002). The subgroup analysis demonstrated that use of DOACs was associated with a significant protective effect in patients aged 75 or older (RR 0.76, 95% CI 0.60–0.97, p = 0.03) and when the time from index stroke to randomization was ≥8 days (RR 0.80, 95% CI 0.66–0.97, p = 0.02) in preventing recurrency of any type of stroke. Conclusions: Our meta-analysis showed lack of overall benefit of anticoagulation with DOAC compared to antiplatelet therapy for recurrent stroke prevention in adult patients with ESUS. However, the subgroup analyses suggest the possibility of interactions between age and timing of randomization since stroke and treatment with an DOAC in terms of recurrent stroke prevention. Further research toward tailoring the antithrombotic strategy according to patient characteristics is needed.
Chronic Nasal Disease and Eustachian Tube Function: What Is the Role of Tubomanometry?
Authors: Anastasiadou Sofia , Bountzis Polyzois , Gkogkos Dimitrios-Evangelos , Karkos Petros , Constantinidis Jannis , Triaridis Stefanos , Psillas George
Published: November 2024

Background/objectives: Eustachian tube dysfunction (ETD) presents complex diagnostic challenges in otolaryngology, compounded by concurrent chronic nasal disease. Patient-reported outcome measures (PROMs) often assess ETD severity due to its elusive diagnosis. Tubomanometry (TMM) emerges as a promising diagnostic tool, yet its application alongside chronic nasal disease remains unclear. Our study aims to elucidate TMM’s role in ETD diagnosis within the context of chronic nasal diseases, integrating subjective assessments, clinical examination, and TMM results. Methods: A prospective observational study was conducted with patients suffering from ETD and chronic nasal disease allocated in three different groups according to their nasal pathology. Clinical examination, PROMs in the form of ETDQ-7, and NOSE questionnaires as well as TMM were performed. Results of the above subjective and objective measurements were analysed and correlated statistically to determine the value of TMM in chronic nasal disease patients. Results: All recruited patients suffered from ETD and chronic nasal disease, with similarly affected ETDQ-7 scores across all groups, while NOSE scores differed significantly based on the underground nasal pathology. TMM values confirm the presence of ETD in all three groups, confirming the role of TMM within this cohort. Interestingly, TMM values can still confirm the presence of ETD in patients with chronic nasal disease but cannot discriminate among chronic nasal pathology patients, making TMM a diagnostic tool with uniformity among the chronic nasal pathologies. Conclusions: ETD in individuals with chronic nasal disease presents distinct complexities, requiring a tailored diagnostic approach. In this context, a thorough clinical assessment, integrating ETDQ-7 and NOSE questionnaires, supplemented by TMM where accessible, is crucial to confirm diagnosis. This study confirms that TMM can diagnose ETD in all nasal pathology patients without being influenced by the nature of the disease. This research endeavours to refine diagnostic strategies, enriching clinical decision-making, and enhancing ETD management in patients suffering with chronic nasal diseases.
Auditory Development of Young Children with Profound Hearing Loss, Cochlear Implants, and Congenital CMV Infection
Authors: Skarzynski Piotr H. , Obrycka Anita , Kolodziejak Aleksandra , Lorens Artur , Gos Elzbieta , Zdanowicz Rita , Skarzynski Henryk
Published: November 2024

Background/Objectives: The aim of this study was to assess auditory development in young children with profound hearing loss, cochlear implants (CIs), and congenital cytomegalovirus (cCMV) infection and to determine the effect of comorbidities on their development. Methods: The study group (cCMV group) consisted of 47 CI children—18 girls and 29 boys—who had been diagnosed as having prelingual hearing loss due to cCMV infection (with or without comorbidities); the mean age at CI activation was 15.2 months (range: 9.7–23.8; SD = 3.5). The reference group (no cCMV) consisted of 117 similar children (57 girls and 60 boys) who had profound sensorineural hearing loss not caused by cCMV infection; they had no comorbidities. The mean age at CI activation in the second group was 14.3 months (range: 7.9–23.5; SD = 4.0). Auditory development in all children was assessed with the LittlEARS Auditory Questionnaire (LEAQ) at CI activation and at about 1, 5, 9, 14, and 24 months of CI use. Results: The mean LEAQ total score increased over a similar time frame from 9.8 pts to 28.9 pts in the cCMV group without comorbidities, from 4.5 pts to 18.5 pts in the cCMV group with comorbidities, and from 9.2 to 31.6 pts in the reference group with no cCMV infection. Conclusions: Early cochlear implantation in children with sensorineural hearing loss due to congenital CMV infection and no comorbidities promotes their early auditory development in a similar way to children without cCMV infection.
Factors Contributing to Early Recovery of Urinary Continence Following Radical Prostatectomy: A Narrative Review
Authors: Barakat Bara , Hadaschik Boris , Al-Nader Mulham , Schakaki Samer
Published: November 2024

Stress urinary incontinence (SUI) is a common condition in patients following radical prostatectomy (RP), which has a significant impact on all aspects of quality of life and is associated with significant social stigma. The factors that improve urinary incontinence in patients following surgery remain controversial. The aim of our narrative review was to identify and synthesise the latest evidence on pre-, intra- and post-operative factors and predictors that contribute to early continence recovery after RP. In this narrative review, primary resources were identified by searching PubMed, EMBASE and Medline, and secondary resources were collected by cross-referencing citations in the relevant articles. We started our review by searching for systematic reviews of factors and predictors that contribute to early recovery of urinary continence after RP. We then reviewed societal guidelines such as the American Urological Association and European Urological Association guidelines on male urinary incontinence. This review focuses on the pre-, intra and postoperative factors that influence postoperative SUI after RP, as well as highlighting modifications in surgical techniques that lead to early continence recovery. Increasing age, higher BMI, shorter membranous urethral length (MUL), and larger PV are independent prognostic factors for SUI within 3 months after RP. Factors such as modified surgical technique preservation of anatomical structure lead to influence postoperative early continence recovery. SUI after RP is influenced by various factors. These factors include not only anatomical landmarks and patient-related factors such as age, BMI, length of MUL and prostate volume, but also prior transurethral resection or laser enucleation of the prostate, the surgeon’s expertise, the surgical approach and NS technique.
Relationships Among the EmPHasis-10 Questionnaire, the Simplified Four-Strata Risk Assessment Tool, and Echocardiographic Parameters in Patients with Precapillary Pulmonary Hypertension
Authors: Varga Andreea , Cristescu Liviu , Iancu Dragos-Gabriel , Dumbrava Robert-Adrian , Moldovan Diana-Andreea , Stoica Florin , Fodor Stefania Raluca , Neagu Claudiu , Suteu Radu Adrian , Tilea Ioan
Published: November 2024

Background/Objectives: Pulmonary arterial hypertension (PAH) and chronic thromboembolic pulmonary hypertension (CTEPH) are complex diseases that require precise diagnosis and management. The ESC risk score has been used in both conditions. We assessed the relationship between the EmPHasis-10 questionnaire (patient subjective evaluation) and objective assessment using endorsed tools (simplified four-strata risk assessment and right ventricular imaging by transthoracic echocardiography). Methods: The present study retrospectively extracted data from 40 adult patients (27 PAH and 13 CTEPH cases) diagnosed in a single center in Romania. The EmPHasis-10 questionnaire and the four-strata risk assessment (FSRA) tool were applied to each patient. Mean pulmonary artery pressure (mPAP), tricuspid annular plane systolic excursion (TAPSE), TAPSE/systolic pulmonary artery pressure (TAPSE/sPAP) ratio, and right ventricular outflow tract acceleration time (RVOT-AT) were assessed. Results: A significant correlation was observed between the EmPHasis-10 scores and the FSRA tool, the WHO functional class, and the 6 min walking distance. Emphasis-10 score did not correlate with any of the echocardiographic parameters. The FSRA tool showed a moderate positive correlation with mPAP (r = 0.42, p = 0.01) and a negative correlation with TAPSE (r = −0.46, p = 0.003); additionally, across the entire cohort, it was moderately negatively correlated with both RVOT-AT (r = −0.42, p = 0.01) and TAPSE/sPAP ratio (r = −0.43, p = 0.005). Conclusions: Our study evidenced the alignment between EmPHasis-10 scores and prognostic risk score, with poorer health-related quality of life corresponding to higher FSRA. The EmPHasis-10 questionnaire proves to be a valuable, easy-to-use instrument, offering meaningful insights into patients’ health-related quality of life, underscoring its utility in enhancing comprehensive patient assessment and management.
Diagnostics and Surgical Treatment of Deep Endometriosis—Real-World Data from a Large Endometriosis Center
Authors: Grube Marcel , Castan Maren , Drechsel-Grau Alexander , Praetorius Teresa , Greif Karen , Staebler Annette , Neis Felix , Rall Katharina , Kraemer Bernhard , Kommoss Stefan , Andress Jürgen
Published: November 2024

Background: Deep endometriosis (DE) is a special form of endometriosis, one of the most common benign diseases in gynecology. In the specific case of DE, ectopic endometrium can be found not only in peritoneal but also in deeper tissue layers or even as parenchymal organ infiltration. Symptoms include dysmenorrhea, dyspareunia, dyschezia, and dysuria, as well as asymptomatic hydronephrosis or other organ dysfunctions. Due to a pathogenesis of the disease that has not been conclusively clarified to date, no causal therapy exists, which is why surgical resection of DE is still the gold standard for symptomatic cases. Methods: This article retrospectively describes the challenges in diagnosis and surgical treatment of DE at a German Level III Endometriosis Center, with a focus on diagnosis and surgical treatment, as well as the analysis of perioperative and postoperative complications. Results: The surgical treatment of DE is performed in most cases by minimally invasive laparoscopy (94.1%), whereas complex procedures such as ureterolysis, adhesiolysis, or preparation of the rectovaginal septum are considered standard procedures as well. The complexity of the procedures is further underlined by a high need for interdisciplinary operations (28%). Despite high complexity, severe postoperative complications occurred in only 3.1% of surgeries, with the complication rate being significantly higher whenever bowel surgery was necessary for DE resection. Conclusions: Our results emphasize the complexity and interdisciplinary nature of the disease. Therefore, treatment should preferably take place at an endometriosis center of the highest level with experienced, well-coordinated teams.
Kidney-Sparing Management of UTUC in Solitary Kidney Patients: A Retrospective Analysis and Narrative Review
Authors: Peteinaris Angelis , Polyzonis Spyridon , Tatanis Vasileios , Spinos Theodoros , Katsakiori Paraskevi , Vrettos Theofanis , Liatsikos Evangelos , Kallidonis Panagiotis
Published: November 2024

Background/Objectives: The aim of this study is the presentation of an endoscopic therapeutic approach for three patients with a solitary kidney who were diagnosed with urothelial cancer of the upper tract. Methods: This retrospective analysis included patients with solitary kidneys who suffered from high-grade UTUC (urothelial cancer of the upper urinary tract) and underwent conservative treatment. Results: The first patient was a 67-year-old male who had a prior history of a nephroureterectomy due to UTUC six years ago. The patient was diagnosed with high-grade UTUC in the contralateral kidney. The tumor has been managed with endoscopic ablation. The second patient was a 74-year-old male with a non-functional kidney and high-grade UTUC diagnosed in the contralateral side. The patient underwent endoscopic ablation for the tumor. The third case was a 68-year-old female patient who had a history of a nephroureterectomy due to UTUC. Afterward, she was diagnosed with high-grade UTUC in the contralateral kidney. The patient was treated with percutaneous tumor resection and the placement of a nephrostomy tube. The first patient was included in an immunotherapy program based on an oncologist consultation after laser ablation treatment for Ta high-grade UTUC, followed by the endoscopic management of two recurrences. Afterward, no recurrence was detected. The remaining two patients followed up without the detection of a new recurrence. Conclusions: The kidney-sparing approach (tumor laser ablation or resection) for high-risk UTUC treatment in selected patients with solitary kidneys seems to provide adequate early outcomes in relation to preserving renal function and effective disease management. It is important to personalize the way of treatment in every case after a thorough examination of the patient’s data.
Tact Training with Augmentative Gestural Support for Language Disorder and Challenging Behaviors: A Case Study in an Italian Community-Based Setting
Authors: Turriziani Laura , Vartellini Rosa , Barcello Maria Grazia , Di Cara Marcella , Cucinotta Francesca
Published: November 2024

Background: Gestures or manual signing are valid options for augmentative and alternative communication. However, the data in the literature are limited to a few neurodevelopmental disorders, and less is known about its application in the community setting. Objectives: This case report explores the feasibility and preliminary efficacy of tact training with augmentative gestural support intervention for a child affected by a language disorder with challenging behaviors in a community setting. Methods: Baseline assessments were conducted using the Verbal Behavior Milestone Assessment and Placement Program (VB-MAPP) and Griffiths Mental Developmental Scale-III (GMDS-III). The patient received six months of standard treatment, consisting of neuropsychomotor and speech therapy each twice a week, with improved cooperation in proposed activities, but no improvement in language. Afterward, a total of 24 sessions of tact training with augmentative gestural support interventions were performed. Data were collected by two independent observers and analyzed to measure language and behavioral outcomes. Results: VB-MAPP scores increased form minimal communication and social interaction at T0 (baseline) to improved compliance but unchanged language skills at T1 (after standard therapy). After tack training with augmentative gestural support (T2), VB-MAPP scores showed significant improvements, with notable increases in verbal operants, independence in communication, and intersubjectivity skills. GMDS-III scores at T2 also demonstrated growth in social, communicative, and cognitive skills. Additionally, challenging behaviors were reduced by more than 70% and nearly resolved by the end of the intervention. Conclusions: Personalized approaches appear to be essential for interventions tailored to developmental age. Further research is needed to determine the effectiveness of these approaches for other neurodevelopmental disorders, identify patient characteristics that may be predictors of outcomes to tailor the intervention, and explore the generalization of the results obtained with these strategies.
Anatomical Measurements of the Malar Bone for Safe Zygomatic Implant Placement: A Study on Donated Bodies
Authors: Barausse Carlo , Felice Pietro , Pistilli Roberto , Pellegrino Gerardo , Bonifazi Lorenzo , Tayeb Subhi , Fazio Antonietta , Marvi Maria Vittoria , Manzoli Lucia , Ratti Stefano
Published: November 2024

Background: The malar bone provides an anchorage point for zygomatic implants, avoiding invasive reconstructive surgeries in the fixed rehabilitation of fully edentulous and severely atrophic maxillae. The limited bone volume, however, requires precise implant placement to prevent complications related to nearby anatomical structures. This observational cross-sectional study aims to measure the malar and zygomatic arch bones and their distances from critical anatomical landmarks to guide surgeons in safe zygomatic implant placement. Methods: Dissections were performed bilaterally on 29 heads from human donated bodies in a cross-sectional observational study. Key landmarks evaluated include the infraorbital foramen (IF), pyriform nasal aperture (PNA), infraorbital margin (IM), zygomaticofacial foramen (ZFF), anterior end (A), and the most protruding point of the zygomatic arch (B). Measurements included IF-PNA, IF-IM, IF-ZFF, ZFF-IM, A-B, and orbital floor depth (OFD). Results: Significant findings showed IF-PNA was greater in males (18.66 ± 2.63 mm, p = 0.001), and IF-ZFF varied between sides (26.72 ± 8.7 mm, p = 0.002). ZFF-IM was larger in males (7.43 ± 2.09 mm, p < 0.001). Heights and thicknesses were also assessed, with significant side differences observed. Conclusions: These findings underscore the importance of understanding precise anatomical distances for successful implant placement. The study provides essential data to enhance surgical planning and training, ensuring safer procedures and minimizing the risk of complications.
Short- and Long-Term Effects of Inhaled Ultrafine Particles on Blood Pressure: A Systematic Review and Meta-Analysis
Authors: Lachowicz Joanna Izabela , Gać Paweł
Published: November 2024

Background: Air pollution contributes to up to 60% of premature mortality worldwide by worsening cardiovascular conditions. Ultrafine particles (UFPs) may negatively affect cardiovascular outcomes, and epidemiological studies have linked them to short- and long-term blood pressure (BP) imbalance. Methods: We conducted a systematic review and meta-analysis of the short- and long-term effects of UFP exposure on systolic (SBP) and diastolic (DBP) blood pressure. Eligibility criteria were established using the Population, Exposure, Comparator, Outcome, and Study Design (PECOS) model, and literature searches were conducted in Web of Science, PubMed, Embase, and Scopus for studies published between 1 January 2013 and 9 October 2024. Risk of Bias (RoB) was assessed following World Health Organization (WHO) instructions. Separate meta-analyses were performed for the short- and long-term effects of UFP exposure on SBP and DBP. Additionally, we analyzed SBP and DBP imbalances across different timespans following short-term exposure. Results: The results showed an increase in BP during short-term UFP exposure, which returned to baseline values after a few hours. Changes in SBP were greater than in DBP following both short- and long-term exposure. Prolonged exposure to UFPs is associated with increased SBP and concurrently low DBP values. Chronic exposure to UFPs may lead to a persistent increase in SBP, even without a concurrent increase in DBP. Conclusions: The findings presented here highlight that UFPs may contribute to worsening cardiovascular outcomes in vulnerable populations living in air-polluted areas.
Resection of Calcified and Giant Thoracic Disc Herniation Through Bilateral Postero-Lateral Approach and 360° Cord Release: A Technical Note
Authors: Zaed Ismail , Pommier Benjamin , Capo Gabriele , Barrey Cédric Y.
Published: November 2024

Background/Objectives: Surgical treatment of thoracic disc herniation (TDH) is risky and technically demanding due to its proximity to the spinal cord and the high possibility of the TDH being calcified (up to 40%), making the resection even more complex. Calcified TDH may be resected from an anterior via thoracotomy/thoracoscopy, lateral extra-cavitary, or a postero-lateral approach. Here, we present our experience in managing such pathology with an original technique Methods: This original technique, used successfully in more than 40 patients, is introduced, with a precise description of the surgical anatomy and the surgical steps to take. Indications for surgical management and neurological outcomes are also analyzed. This surgical approach consisted of transverso-pediculectomy, most often bilaterally, partial vertebral body drilling, 360° release of the cord, and short fixation. Results: A total of 44 patients were collected, with a mean age of 52.4 ± 11.7 years. Seven patients (15.9%) had complete calcifications, and thirty-one had partial calcifications (70.5%), while the remaining six did not have signs of calcifications. There were only 4 intraoperative complications (2 dural tears and 2 loss of evoked potentials). The TDH could be resected in total for 39 patients (88.6%) and partially, according to the “floating” technique, in 5 patients (11.4%). In the postoperative follow-up, all of the patients except two (presenting with sensory aggravation) reported an improvement in neurological conditions leading to an overall risk of neurological aggravation of 4.5%. Conclusions: The bilateral postero-lateral approach provides a large decompression of the cord (360°) and gives safe access to the TDH, even calcified, permitting high rates of total resection. It also prevents any prejudicial pressure on the spinal cord, reducing the risk of severe postoperative deficits and permitting optimal instrumentation (pedicle screw-based) of the spinal segment. The surgical sequence to resect the bony structures around the spinal cord is of great importance.
Inferring TLB Configuration with Performance Tools
Authors: Agredo Cristian , Langehaug Tor J. , Graham Scott R.
Published: November 2024

Modern computing systems are primarily designed for maximum performance, which inadvertently introduces vulnerabilities at the micro-architecture level. While cache side-channel analysis has received significant attention, other Central Processing Units (CPUs) components like the Translation Lookaside Buffer (TLB) can also be exploited to leak sensitive information. This paper focuses on the TLB, a micro-architecture component that is vulnerable to side-channel attacks. Despite the coarse granularity at the page level, advancements in tools and techniques have made TLB information leakage feasible. The primary goal of this study is not to demonstrate the potential for information leakage from the TLB but to establish a comprehensive framework to reverse engineer the TLB configuration, a critical aspect of side-channel analysis attacks that have previously succeeded in extracting sensitive data. The methodology involves detailed reverse engineering efforts on Intel CPUs, complemented by analytical tools to support TLB reverse engineering. This study successfully reverse-engineered the TLB configurations for Intel CPUs and introduced visual tools for further analysis. These results can be used to explore TLB vulnerabilities in greater depth. However, when attempting to apply the same methodology to the IBM Power9, it became clear that the methodology was not transferable, as mapping functions and performance counters vary across different vendors.
Synthesis, Functionalization, and Biomedical Applications of Iron Oxide Nanoparticles (IONPs)
Authors: Salehirozveh Mostafa , Dehghani Parisa , Mijakovic Ivan
Published: November 2024

Iron oxide nanoparticles (IONPs) have garnered significant attention in biomedical applications due to their unique magnetic properties, biocompatibility, and versatility. This review comprehensively examines the synthesis methods, surface functionalization techniques, and diverse biomedical applications of IONPs. Various chemical and physical synthesis techniques, including coprecipitation, sol–gel processes, thermal decomposition, hydrothermal synthesis, and sonochemical routes, are discussed in detail, highlighting their advantages and limitations. Surface functionalization strategies, such as ligand exchange, encapsulation, and silanization, are explored to enhance the biocompatibility and functionality of IONPs. Special emphasis is placed on the role of IONPs in biosensing technologies, where their magnetic and optical properties enable significant advancements, including in surface-enhanced Raman scattering (SERS)-based biosensors, fluorescence biosensors, and field-effect transistor (FET) biosensors. The review explores how IONPs enhance sensitivity and selectivity in detecting biomolecules, demonstrating their potential for point-of-care diagnostics. Additionally, biomedical applications such as magnetic resonance imaging (MRI), targeted drug delivery, tissue engineering, and stem cell tracking are discussed. The challenges and future perspectives in the clinical translation of IONPs are also addressed, emphasizing the need for further research to optimize their properties and ensure safety and efficacy in medical applications. This review aims to provide a comprehensive understanding of the current state and future potential of IONPs in both biosensing and broader biomedical fields.
Effect of the ActivaMotricidad Program on Improvements in Executive Functions and Interpersonal Relationships in Early Childhood Education
Authors: Ureña Ortín Nuria , Madinabeitia Cabrera Iker , Alarcón López Francisco
Published: November 2024

Background: The objective of this study was to analyze the impacts of a comprehensive physical exercise program with cognitive involvement during the school day on the executive functions and interpersonal skills of 5- and 6-year-old children. Methods: A total of 68 children participated in a 3-week pre–post intervention. Results: The results showed significant improvements in executive functions and interpersonal skills under the experimental conditions compared to the control group. These positive effects were observed to persist after a three-week follow-up. Conclusions: Therefore, the ActivaMotricidad program, which focuses on developing fundamental motor skills through cognitive, cooperative, and coordinative challenges, can serve as a useful and low-cost tool to achieve the objectives of comprehensive development and sustainability for children in early childhood education.
Examining Working Memory Training for Healthy Adults—A Second-Order Meta-Analysis
Authors: Syed Maria , Lum Jarrad A. G. , Byrne Linda K. , Skvarc David
Published: November 2024

Background: Enhancing working memory performance in cognitively and physically healthy individuals is a popular area of research. The results from a large number of studies have now been summarized in multiple meta-analyses. In these reviews, various training methods have been examined, including mindfulness training, adaptive working memory training, physical activity training, and video game training, to examine whether working memory capacity can be improved. This report aggregated the results of these meta-analyses using second-order meta-analytic approaches to ascertain the extent to which working memory functioning can be enhanced in healthy adults. Methods: A total of six meta-analyses of randomized controlled trials that compared working memory interventions to a control group were included in the analyses. These studies were identified after systematically searching three electronic databases: APA PsycInfo, ERIC and Medline. Collectively, the meta-analyses investigated the effects of cognitive programs, mindfulness, video games and physical activity on working memory. Only meta-analyses undertaken with healthy adults aged between 18 and 55 years were included in the report. Results: The results revealed an average improvement in working memory across the included studies compared to the control groups. The findings indicated a small yet significant enhancement in working memory, with a standardized mean difference of 0.335 (95% CI [0.223; 0.447], p < .001). Further analysis tests for superiority effects between the different working memory training programs revealed no significant differences between intervention effect sizes. Conclusion: Based on the findings, it can be concluded that the working memory capacity of healthy adults can be improved through training. However, the effect size is small, so the utility of this type of training in real-life improvements in cognition may be minimal. The evidence does not indicate that one type of working memory training is superior to another.
Numerical Simulation and Experimental Validation of the Acoustical Target Strength of Bluefin Tuna Swimbladders Derived from 3D Computed Tomographic Images
Authors: Ladino Anderson , Pérez-Arjona Isabel , Espinosa Victor , Puig-Pons Vicent , Gándara Fernando de la , Ortega Aurelio , Blanco Edurne , Godinho Luis
Published: November 2024

The swimbladder, when present, is the main contributor to the acoustical target strength (TS) of fish. Numerical modeling of target strength must include swimbladder dimensions, orientation, and shape for the proper estimation of target strength and its directivity. Several Atlantic Bluefin tuna (Thunnus thynnus, ABFT) specimens between 90 and 100 cm of fork length were studied by performing computed tomographic (CT) post-mortems in both fresh and frozen states. ABFT swimbladder 3D models were derived for the first time to be compared with experimental TS measurements through numerical simulation methods, using the Method of Fundamental Solutions (MFS). The numerical estimation (−23.3 dB) agreed with the experimental measurement of TS (−22.1 dB) performed in a tank with tuna with a mean fork length of 100 cm, showing the importance of considering realistic swimbladder shapes and swimming behavior in the numerical simulation of TS.
Natural and Human Impacts on Coastal Areas
Authors: Asensio-Montesinos Francisco , Molina Rosa , Anfuso Giorgio , Manno Giorgio , Lo Re Carlo
Published: November 2024

Coasts are the most densely populated regions in the world and are vulnerable to different natural and human factors, e.g., sea-level rise, coastal accretion and erosion processes, the intensification of sea storms and hurricanes, the presence of marine litter, chronic pollution and beach oil spill accidents, etc. Although coastal zones have been affected by local anthropic activities for decades, their impacts on coastal ecosystems is often unclear. Several papers are presented in this Special Issue detailing the interactions between natural processes and human impacts in coastal ecosystems all around the world. A better understanding of such natural and human impacts is therefore of great relevance to confidently predict their negative effects on coastal areas and thus promote different conservation strategies. The implementation of adequate management measures will help coastal communities adapt to future scenarios in the short and long term and prevent damage due to different pollution types, e.g., beach oil spill accidents, through the establishment of Environmental Sensitivity Maps.
A Data-Driven Model for Rapid CII Prediction
Authors: Mühmer Markus , La Ferlita Alessandro , Geber Evangelos , Ehlers Sören , Di Nardo Emanuel , El Moctar Ould , Ciaramella Angelo
Published: November 2024

The shipping industry plays a crucial role in global trade, but it also contributes significantly to environmental pollution, particularly in regard to carbon emissions. The Carbon Intensity Indicator (CII) was introduced with the objective of reducing emissions in the shipping sector. The lack of familiarity with the carbon performance is a common issue among vessel operator. To address this aspect, the development of methods that can accurately predict the CII for ships is of paramount importance. This paper presents a novel and simplified approach to predicting the CII for ships, which makes use of data-driven modelling techniques. The proposed method considers a restricted set of parameters, including operational data (draft and speed) and environmental conditions, such as wind speed and direction, to provide an accurate prediction of the CII factor. This approach extends the state of research by applying Deep Neural Networks (DNNs) to provide an accurate CII prediction with a deviation of less than 6% over a considered time frame consisting of different operating states (cruising and maneuvering mode). The result is achieved by using a limited amount of training data, which enables ship owners to obtain a rapid estimation of their yearly rating prior to receiving the annual CII evaluation.
Hydrodynamic Research of Marine Structures
Authors: Konispoliatis Dimitrios N. , Mavrakos Spyros A.
Published: November 2024

Hydrodynamics plays a crucial role in the design and analysis of marine structures, as it involves the study of the motion of fluid and its interaction with various types of structures in a marine environment [...]
Unraveling the Potential of Yarrowia lipolytica to Utilize Waste Motor Oil as a Carbon Source
Authors: Miranda Sílvia M. , Belo Isabel , Lopes Marlene
Published: November 2024

This study evaluated the potential of Y. lipolytica (CBS 2075 and DSM 8218) to grow in waste motor oil (WMO) and produce valuable compounds, laying the foundation for a sustainable approach to WMO management. Firstly, yeast strains were screened for their growth on WMO (2–10 g·L−1) in microplate cultures. Despite limited growth, the CBS 2075 strain exhibited comparable growth to control conditions (without WMO), while DSM 8218 growth increased 2- and 3-fold at 5 g·L−1 and 10 g·L−1 WMO, respectively. The batch cultures in the bioreactor confirmed the best performance of DSM 8218. A two-stage fed-batch strategy–growth phase in aliphatic hydrocarbons, followed by the addition of WMO (one pulse of 5 g·L−1 or five pulses of 1 g·L−1 WMO), significantly increased biomass production and WMO assimilation by both strains. In experiments with five pulses, CBS 2075 and DSM 8218 strains reached high proteolytic activities (593–628 U·L−1) and accumulated high quantities of intracellular lipids (1.3–1.7 g·L−1). Yeast lipids, mainly composed of oleic and linoleic acids with an unsaturated/saturated fraction > 59%, meet the EU biodiesel standard EN 14214, making them suitable for biodiesel production.
Spatiotemporal Pattern of a Macrofungal Genus Phylloporia (Basidiomycota) Revealing Its Adaptive Evolution in China
Authors: Wang Xue-Wei , Zhou Li-Wei
Published: November 2024

The understanding of distribution and the evolutionary scenario is crucial for the utilization and conservation of biological resources; nevertheless, such explorations rarely focus on macrofungi. The current study selects a macrofungal genus, Phylloporia, and explores its spatiotemporal pattern in China. A total of 117 available occurrence records of Phylloporia in China were summarized for the current analyses. Ensemble modeling supports the highly suitable habitat of Phylloporia concentrated in southern, especially southeastern, China, where the ancestor of Phylloporia originated 77.74 million years ago and then dispersed to other parts of China. Benefitting from the available suitable habitats, Phylloporia rapidly diversified after its divergence in Southeast China. Then, the net diversification rate slowed down when the rapidly diversifying species filled available niches in Southeast China and the dispersed species in other parts of China inhabited the less suitable and unsuitable habitats. During adaptive evolution, precipitation, temperature and the host plant are the major environmental variables that shape the spatiotemporal pattern of Phylloporia. In conclusion, the current study reveals the adaptive evolutionary scenario of Phylloporia and provides the first exploration of the spatiotemporal pattern of macrofungi.
Key Amino Acid Residues of the Agt1 Transporter for Trehalose Transport by Saccharomyces cerevisiae
Authors: Chen Anqi , Cheng Yuhan , Meng Liushi , Chen Jian
Published: November 2024

Trehalose is crucial for the stress resistance of Saccharomyces cerevisiae, primarily through its stabilization of proteins and membranes. The Agt1 transporter, a member of the Major Facilitator Superfamily, mediates trehalose uptake, a key process for maintaining cellular integrity under stress. Despite its importance, the molecular mechanisms of Agt1-mediated trehalose transport remain underexplored. In this study, we expressed and purified the trehalase enzyme TreA from E. coli to develop reliable trehalose assays. We screened 257 wild S. cerevisiae isolates, identifying strains with enhanced trehalose transport capacities. Comparative analyses, including structural modeling and molecular docking, revealed that specific Agt1 variants exhibited significantly higher transport efficiency, influenced by key residues in the transporter. Molecular dynamics simulations and steered molecular dynamics provided further insights, particularly into the role of the Agt1 channel head region in substrate recognition and binding. Site-directed mutagenesis validated these findings, showing that mutations at critical residues, such as 156Q, 164L, 256Q, 395E, 396R, and 507Y significantly reduced transport activity, while 137Q, 230T, and 514 N increased efficiency under certain conditions.
GSDMB Gene Polymorphisms and Their Association with Asthma Susceptibility: A Systematic Review and Meta-Analysis of Case–Control Studies
Authors: Ramos-Nino Maria E. , Ramdass Prakash V. A. K.
Published: November 2024

Background: Asthma is a respiratory disorder influenced by genetic and environmental factors. The rs7216389 polymorphism in the gasdermin B (GSDMB) gene on chromosome 17q21 has been implicated in asthma susceptibility with conflicting results. This meta-analysis aims to bring forward new findings on the association between this polymorphism and asthma across diverse populations and its potential as a genetic marker for asthma risk. Methods: A systematic review and meta-analysis were conducted through March 2024, and odd ratios were calculated. Results: The meta-analysis included 22 studies with a total of 9012 asthma patients and 11,657 controls. The results show an OR = 1.24, 95% CI [1.13, 1.37], p < 0.00001. Subgroup analyses stratified by age and ethnicity between asthma patients with T alleles vs. C alleles demonstrated an association between having the T allele and asthma susceptibility across Asian, Caucasian, and American minorities, but not among Arabs. Young asthma patients with the dominant allele (T) showed higher asthma risk than those with C allele or heterozygote TC, and to a higher extent than for adults. Conclusions: This meta-analysis indicates the importance of genetic factors in asthma in certain ethnicities and underscores the potential utility of the rs7216389 polymorphism as a genetic marker for asthma risk assessment.
Maternal Serum SCUBE-1: A Novel Ischemic Marker in Preeclampsia
Authors: Dinc Gulseren , Karahan Suleyman Caner , Guven Suleyman
Published: November 2024

Background: SCUBE-1 (Signal peptide-CUB (complement C1r/C1s, Uegf, and Bmp1)-EGF (epidermal growth factor)-domain-containing protein 1) is a novel marker of ischemia, which is a cell surface-secreted protein in the platelets and endothelial cells. The aim of the study is to measure serum SCUBE-1 levels and investigate their association with uteroplacental blood flow in patients with preeclampsia. Methods: The study was conducted on patients with preeclampsia. Maternal serum SCUBE1 and IMA levels were the main outcomes. The control group consisted of gestational-age-matched pregnant women. Fetal umbilical artery (UA) pulsatility index (PI), middle cerebral artery PI, cerebroplacental ratio (CPR), and maternal uterine artery (UtA)-PI were also examined, and correlation analysis was performed to reveal the association between maternal serum SCUBE1 levels and Doppler findings. Results: The study group consisted of thirty-two preeclamptic patients, and the control group consisted of thirty-two uncomplicated singleton pregnancies. Maternal serum SCUBE1 and IMA levels were significantly higher in preeclamptic women compared to the control group (p < 0.000, p < 0.004, respectively). Mean UtA-PI values and fetal UA-PI values were significantly higher in preeclamptic pregnant women compared to the control group (p < 0.05, p < 0.05, respectively). However, the average CPR was significantly lower in pregnant women with preeclampsia (p < 0.05). While no significant correlation was found between maternal serum SCUBE1 levels and UA-PI and CPR (p > 0.05, p > 0.05, respectively), a significant correlation was found between right and left UtA-PI (p < 0.004, p < 0.006, respectively). Conclusions: The maternal serum SCUBE1 level is increased in patients with preeclampsia, and this increase is significantly correlated with the maternal uterine artery pulsatility index.