bluuebunny/crossref_metadata_2025 huggingface dataset:
The Genetic Basis of Natural Variation in Drosophila melanogaster Immune Defense against Enterococcus faecalis
Authors: Chapman Joanne R , Dowell Maureen A , Chan Rosanna , Unckless Robert L
Published: February 2020

Dissecting the genetic basis of natural variation in disease response in hosts provides insights into the coevolutionary dynamics of host-pathogen interactions. Here, a genome-wide association study of Drosophila melanogaster survival after infection with the Gram-positive entomopathogenic bacterium Enterococcus faecalis is reported. There was considerable variation in defense against E. faecalis infection among inbred lines of the Drosophila Genetics Reference Panel. We identified single nucleotide polymorphisms associated with six genes with a significant (p < 10−08, corresponding to a false discovery rate of 2.4%) association with survival, none of which were canonical immune genes. To validate the role of these genes in immune defense, their expression was knocked-down using RNAi and survival of infected hosts was followed, which confirmed a role for the genes krishah and S6k in immune defense. We further identified a putative role for the Bomanin gene BomBc1 (also known as IM23), in E. faecalis infection response. This study adds to the growing set of association studies for infection in Drosophila melanogaster and suggests that the genetic causes of variation in immune defense differ for different pathogens.
The Effects of Slope Initialization on the Numerical Model Predictions of the Slope-Vegetation-Atmosphere Interaction
Authors: Tagarelli Vito , Cotecchia Federica
Published: February 2020

Deep slope movements and, eventually, slope failure, have been often interpreted to be due to slope-vegetation-atmosphere interaction on slopes formed of clayey materials in the Italian Southern-Eastern Apennines, as reported in the literature. Such slopes are generally formed of flysch, within which clay is the main lithotype. Such clays are characterized by a disturbed meso-fabric, as an effect of the intense tectonics. The paper presents the results of coupled hydromechanical numerical analyses of the slope-vegetation-atmosphere interaction for a clay slope representative for the geomechanical scenario where such climate-induced deep slope movements have been repeatedly recorded. In the analyses, different model initialization procedures and parameter values were adopted. The comparison of the numerical results with the site data is aimed at assessing the effects of the soil-vegetation-atmosphere interaction taking place in the top strata of the slope, on the stress-strain conditions across the whole slope, and on the slope stability. The comparison between the numerical results of the analyses carried out entailing different initialization stages are intended to evaluate the influence of such a stage on the model predictions. It is found that only when the slope model initialization accounts for the slope loading history, developed over geological time, the numerical predictions get close to the site observations. In such case, the numerical results confirm that deep movements consequent to progressive failure may take place in clay slopes due to the slope-vegetation-atmosphere interaction.
Chemical and Mechanical Differences between Historic and Modern Scots Pine Wood
Authors: Hudson-McAulay Kate , Kennedy Craig J. , Jarvis Michael C.
Published: February 2020

Timber is one of the most common historic building materials, but relatively little is known about how it ages in situ. Here we investigate historic and modern Scots pine to determine any chemical or mechanical differences between them. Fourier-transform infrared (FTIR) microscopy was used to investigate differences in the chemical composition of Scots pine (Pinus sylvestris L.) timber, comparing small samples from historic beams about 500 years old with modern timber. The hemicellulosic acetyl content was reduced by about half in the historic samples, uniformly across the thickness of the beams. A chemical mechanism was therefore suggested for the loss of acetyl groups, as has been observed in paper. In paper, deacetylation and the resulting release of acetic acid are accompanied by loss of strength. Mechanical testing of the historic timber was difficult because the available length of the samples along the grain was only 20 mm. After developing a miniaturized compression test developed for the purpose, it was shown that the relative stiffness of the historic Scots pine samples was reduced by about half compared to modern material.
Assessment of Erosion in River Basins: A Distributed Model to Estimate the Sediment Production over Watersheds by a 3-Dimensional LS Factor in RUSLE Model
Authors: Covelli Carmine , Cimorelli Luigi , Pagliuca Danila Nicole , Molino Bruno , Pianese Domenico
Published: February 2020

Erosive processes influence on several phenomena. In particular, they could influence on land depletion, on vegetation weakening, on aggradation phenomena of intermediate, and plain reaches of rivers, on waterways interruption due to overaggradation phenomena caused by floods, and on the losses of water volumes that may be stored in reservoirs. Among the models proposed in the literature for the prediction of erosion on the annual scale, one of the most widely used is the Revised Universal Soil Loss Equation (RUSLE). In the present paper, starting from the definition of the original model, the authors improved the important combined slope length and slope angle (LS-factor), taking into account the mutual interaction of solid particles, in terms of path and confluences, so as to transform the model, which was first classified on a slope scale or at most on a parcel one, into a distributed model on a basin scale. The use of a distributed approach is an integral part of the analysis of the hydrogeological risk. In this way, it is possible to obtain a map of the erodibility of any basin, from which to derive the most vulnerable areas. The proposed methodology has been tested on the Camastra Basin, located in Basilicata Region of Southern Italy.
The Impact of Acute Kidney Injury in the Perioperative Period on the Incidence of Postoperative Delirium in Patients Undergoing Coronary Artery Bypass Grafting—Observational Cohort Study
Authors: Kotfis Katarzyna , Ślozowska Justyna , Listewnik Mariusz , Szylińska Aleksandra , Rotter Iwona
Published: February 2020

Recent data indicate that acute kidney damage leads to inflammation in the brain and other distant organs. The purpose of this study was to investigate the effect of acute kidney injury (AKI) according to the Kidney Disease Improving Global Outcome (KDIGO) criteria on the occurrence of postoperative delirium in patients undergoing coronary artery bypass grafting (CABG). We performed a retrospective cohort analysis that included all consecutive patients undergoing elective CABG. The CAM-ICU (Confusion Assessment Method for Intensive Care Unit) was used for delirium assessment. Patients were divided into four groups, depending on the occurrence of AKI in the perioperative period according to KDIGO criteria. Overall, 902 patients were included in the final analysis, the mean age was 65.95 ± 8.01 years, and 76.83% were males (693/957). The majority of patients presented with normal kidney function-baseline creatinine level of 0.91 ± 0.21 (mg/dL). The incidence of AKI in the perioperative setting was 22.17% (200/902). Postoperative delirium was diagnosed in 115/902 patients (12.75%). Compared with no AKI, the odds of developing POD were increased for KDIGO stage 1 (OR 2.401 (95% confidence interval 1.484–3.884), p < 0.001); KDIGO stage 2 (OR 3.387 (95% confidence interval 1.459–7.866), p = 0.005); and highest for KDIGO stage 3 (OR equal to 9.729 (95% confidence interval 2.675–35.382), p = 0.001). Acute kidney injury, based on AKI staging, should be regarded as an independent risk factor for postoperative delirium after cardiac surgery.
An Extension of the Failure Mode and Effect Analysis with Hesitant Fuzzy Sets to Assess the Occupational Hazards in the Construction Industry
Authors: Heidary Dahooie Jalil , Vanaki Amir Salar , Firoozfar Hamid Reza , Zavadskas Edmundas Kazimieras , Čereška Audrius
Published: February 2020

The construction industry is considered as one of the most dangerous industries in terms of occupational safety and has a high rate of occupational incidents and risks compared to other industries. Given the importance of identifying and assessing the occupational hazards in this industry, researchers have conducted numerous studies using statistical methods, multi-criteria decision-making methods, expert-based judgments, and so on. Although, these researchers have used linguistic variables, fuzzy sets and interval-valued intuitionistic fuzzy sets to overcome challenges such as uncertainty and ambiguity in the risk assessment conducted by experts; the previous models lack in efficiency if the experts are hesitant in their assessment. This leads to the inability to assign a specific membership degree to any risk. Therefore, in this research, it is tried to provide an improved approach to the Failure Mode and Effects Analysis (FMEA) method using an Multi-Criteria Decision-Making (MCDM) method based on the hesitant fuzzy set, which can effectively cope with the hesitance of the experts in the evaluation. Also, Stepwise Weight Assessment Ratio Analysis (SWARA) method is applied for risk factor weighing in the proposed approach. This model is applied to a construction industry case study to solve a realistic occupational risk assessment. Moreover, a comparison is made between the results of this model and those obtained by the conventional FMEA and some other aggregation operators. The results indicate that the newly developed approach is useful and flexible to address complex FMEA problems and can generate logical and reliable priority rankings for failure modes.
Prenatal Ambient Particulate Matter Exposure and Longitudinal Weight Growth Trajectories in Early Childhood
Authors: Rosofsky Anna S. , Fabian M. Patricia , Ettinger de Cuba Stephanie , Sandel Megan , Coleman Sharon , Levy Jonathan I. , Coull Brent A. , Hart Jaime E. , Zanobetti Antonella
Published: February 2020

Air pollution exposure during pregnancy has been associated with impaired fetal growth and postnatal weight gain, but few studies have examined the effect on weight growth trajectories. We examine the association between validated 1 km2 resolution particulate matter (PM2.5) concentrations, averaged over pregnancy, and sex-specific growth trajectories from birth to age six of participants in the Boston-based Children’s HealthWatch cohort (4797 participants, 84,283 measures). We compared weight trajectories, predicted using polynomial splines in mixed models, between prenatal PM2.5 above or below the median (9.5 µg/m3), and examined birth weight as an effect modifier. Females exposed to average prenatal PM2.5 ≥ 9.5 µg/m3 had higher weights compared to females exposed to < 9.5 µg/m3 throughout the study period (0.16 kg at 24 months, 0.61 kg at 60 months). In males, higher prenatal PM2.5 exposure was associated with significantly lower weights after 24 months of age, with differences increasing with time (−0.17 at 24 months, −0.72 kg at 60 months). Associations were more pronounced among low birth weight (<2500 g) females, but did not differ by birth weight status in males. Our findings demonstrate the complex association between air pollution exposures and childhood weight trajectories and emphasize the importance of sex-stratified analyses.
How Did Parents View the Impact of the Curriculum-Based HealthLit4Kids Program Beyond the Classroom?
Authors: Nash Rosie , Cruickshank Vaughan , Flittner Anna , Mainsbridge Casey , Pill Shane , Elmer Shandell
Published: February 2020

The HealthLit4Kids program aims to build health literacy in a participatory and contextually relevant way. Whole-of-school and curriculum strategies aim to empower and build capacity to make informed health choices amongst students, teachers, parents, and their local community. The aim of this study was to evaluate the HealthLit4Kids program from the perspective of parents, using a Self-Determination Theory framework. This is one component within a larger evaluation of the program. Parents at four Australian primary schools were interviewed post-program. Qualitative data collected through parent interviews were analyzed thematically to identify themes, and coding checks were completed by experienced qualitative researchers. The three key themes identified were student engagement, behaviour change, and parent engagement. Findings also indicated that parents placed a high value on effective communication from schools and raised a range of health areas such as food and nutrition, physical activity, and mental health with the interviewer. Parent opinions of the HealthLit4Kids program were positive, with many reporting a perceived increase in their children’s ability to understand, communicate and act on health-related knowledge at home. The HealthLit4Kids program requires further research to determine its viability as an optimal pedagogical strategy for the health literacy development of primary school-aged children.
Does Digital Nature Enhance Social Aspirations? An Experimental Study
Authors: van Houwelingen-Snippe Josca , van Rompay Thomas J. L. , de Jong Menno D. T. , Ben Allouch Somaya
Published: February 2020

Loneliness and social isolation are increasingly recognized as important challenges of our times. Inspired by research hinting at beneficial effects of interacting with nature on social connectedness and opportunities provided by ambient technology to simulate nature in a rich and engaging manner, this study explored to what extent digital nature projections can stimulate social aspirations and related emotions. To this end, participants (N = 96) were asked to watch, individually or in pairs, digital nature projections consisting of animated scenes which were either dense or spacious and depicting either wild or tended nature. Subsequently, they filled out a questionnaire comprising measures for social aspirations, awe and fascination. Results show that spacious scenes elicited significantly higher social aspiration and awe scores, especially when watching alone. Design implications are discussed for making digital nature accessible for people with limited access to real nature.
Residential Radon and Histological Types of Lung Cancer: A Meta-Analysis of Case‒Control Studies
Authors: Li Cong , Wang Chunhong , Yu Jun , Fan Yongsheng , Liu Duanya , Zhou Wenshan , Shi Tingming
Published: February 2020

Epidemiological studies on residential radon exposure and the risk of histological types of lung cancer have yielded inconsistent results. We conducted a meta-analysis on this topic and updated previous related meta-analyses. We searched the databases of Cochrane Library, Embase, PubMed, Web of Science and Chinese National Knowledge Infrastructure for papers published up to 13 November 2018. The pooled odds ratio (OR) and 95% confidence interval (CI) were calculated using fixed and random effects models. Subgroup and dose‒response analyses were also conducted. This study was registered with PROSPERO (No. CRD42019127761). A total of 28 studies, which included 13,748 lung cancer cases and 23,112 controls, were used for this meta-analysis. The pooled OR indicated that the highest residential radon exposure was significantly associated with an increased risk of lung cancer (OR = 1.48, 95% CI = 1.26–1.73). All histological types of lung cancer were associated with residential radon. Strongest association with small-cell lung carcinoma (OR = 2.03, 95% CI = 1.52–2.71) was found, followed by adenocarcinoma (OR = 1.58, 95% CI = 1.31–1.91), other histological types (OR = 1.54, 95% CI = 1.11–2.15) and squamous cell carcinoma (OR = 1.43, 95% CI = 1.18–1.74). With increasing residential radon levels per 100 Bq/m3, the risk of lung cancer, small-cell lung carcinoma and adenocarcinoma increased by 11%, 19% and 13%, respectively. This meta-analysis provides new evidence for a potential relationship between residential radon and all histological types of lung cancer.
Frequent Karaoke Training Improves Frontal Executive Cognitive Skills, Tongue Pressure, and Respiratory Function in Elderly People: Pilot Study from a Randomized Controlled Trial
Authors: Miyazaki Atsuko , Mori Hayato
Published: February 2020

We tested whether karaoke training improves cognitive skills and reduces the risk of physical function impairments. We conducted a single-blinded randomized controlled trial in 26 elderly participants at residential care facilities, who were generally healthy or required the lowest level of care. Participants were near the threshold for mild cognitive impairment with the Montreal Cognitive Assessment (MoCA) and close to the sarcopenia cut-off with the skeletal muscle mass index. Pulmonary function as measured with spirometry and tongue strength were used as markers for physical functions affected by sarcopenia. Karaoke training occurred once a week for two hours, with an hour of homework assigned weekly. Karaoke training significantly improved the Frontal Assessment Battery at bedside (FAB) compared with an active control group receiving scratch art training (F = 8.04, permutation p-value = 0.013). Subscore improved with inhibitory control (F = 7.63, permutation p-value = 0.015) and sensitivity to interference (F = 11.98, permutation p-value = 0.001). We observed improved tongue pressure (F = 4.49, permutation p-value = 0.040) and pulmonary function by a greater increase in FIV1 (F = 5.22, permutation p-value = 0.047). Engaging elderly people, especially those in care homes, with karaoke training exercises that are moderately physically challenging may be a key to slowing cognitive decline and preventing dysphagia by sarcopenia.
Occupational Tuberculosis Among Laboratory Workers in South Africa: Applying a Surveillance System to Strengthen Prevention and Control
Authors: Garnett Jennica , Jones David , Chin Graham , Spiegel Jerry M. , Yassi Annalee , Naicker Nisha
Published: February 2020

Background: Tuberculosis (TB) is recognized as an important health risk for health workers, however, the absence of occupational health surveillance has created knowledge gaps regarding occupational infection rates and contributing factors. This study aimed to determine the rates and contributing factors of active TB cases in laboratory healthcare employees at the National Health Laboratory Service (NHLS) in South Africa, as identified from an occupational surveillance system. Methods: TB cases were reported on the Occupational Health and Safety Information System (OHASIS), which recorded data on occupation type and activities and factors leading to confirmed TB. Data collected from 2012 to 2019 were used to calculate and compare TB risks within NHLS occupational groups. Results: During the study period, there were 92 cases of TB identified in the OHASIS database. General workers, rather than skilled and unskilled laboratory workers and medical staff, had the highest incidence rate (422 per 100,000 person-years). OHASIS data revealed subgroups that seemed to be well protected, while pointing to exposure situations that beckoned policy development, as well as identified subgroups of workers for whom better training is warranted. Conclusions: Functional occupational health surveillance systems can identify subgroups most at risk as well as areas of programme success and areas where increased support is needed, helping to target and monitor policy and procedure modification and training needs.
Chained Risk Assessment for Life-Long Disease Burden of Early Exposures–Demonstration of Concept Using Prenatal Maternal Smoking
Authors: Rumrich Isabell K. , Vähäkangas Kirsi , Viluksela Matti , Hänninen Otto
Published: February 2020

Traditional risk factors and environmental exposures only explain less than half of the disease burden. The developmental origin of the health and disease (DOHaD) concept proposes that prenatal and early postnatal exposures increase disease susceptibility throughout life. The aim of this work is to demonstrate the application of the DOHaD concept in a chained risk assessment and to provide an estimate of later in life burden of disease related to maternal smoking. We conducted three systematic literature searches for meta-analysis and reviewed the literature reporting meta-analyses of long-term health outcomes associated with maternal smoking and intermediate risk factors (preterm birth, low birth weight, childhood overweight). In the chained model the three selected risk factors explained an additional 2% (34,000 DALY) of the total non-communicable disease burden (1.4 million DALY) in 2017. Being overweight in childhood was the most important risk factor (28,000 DALY). Maternal smoking was directly associated with 170 DALY and indirectly via the three intermediate risk factors 1000 DALY (1200 DALY in total). The results confirm the potential to explain a previously unattributed part of the non-communicable diseases by the DOHAD concept. It is likely that relevant outcomes are missing, resulting in an underestimation of disease burden.
Activity Demands and Speed Profile of Young Female Basketball Players Using Ultra-Wide Band Technology
Authors: Reina María , García-Rubio Javier , Ibáñez Sergio J.
Published: February 2020

Performance profiles have begun to be identified as extremely useful in order to help coaches individualize training according to the age and gender of athletes. Therefore, the aim of this study was to determine the activity demands and speed profile of U18 female basketball players during competitive matches. Time variables (real and playing time), distance variables (distance performed, distance in speed zones, high intensity distance and distance covered sprinting) and speed variables (number of sprints, sprint duration, maximum speed and average speed) were recorded from forty-eight players belonging to four teams (13 guards, 22 forwards and 13 centers). WIMUPROTM inertial measurement units with ultra-wide band (UWB) indoor-tracking technology recorded six matches during final four in the season 2018/2019. A one factor ANOVA with Cohen’s effect sizes (d) were used to identify the differences between groups (playing position and match day). Distance per minute (123.96 vs 112.67 m), high intensity distance per minute (15.48 vs 14 m), running distance (403.2 vs 541.28 m) and average speed (5.05 vs 5.41 km/h) were significantly higher on day 3 than 1, respectively. About playing position, forwards played more minutes during games, so covered a greater distance, more sprints and high intensity actions than the rest. In spite of fatigue, day 3 showed a greater intensity than day 1, therefore, the last day was the crucial one for the teams in the tournament. Forwards when playing more minutes obtain higher absolute values ​​but not per minute which could mean a lower performance of the team.
Occupational Exposure to Dust Produced when Milling Thermally Modified Wood
Authors: Očkajová Alena , Kučerka Martin , Kminiak Richard , Krišťák Ľuboš , Igaz Rastislav , Réh Roman
Published: February 2020

During production, thermally modified wood is processed using the same machining operations as unmodified wood. Machining wood is always accompanied with the creation of dust particles. The smaller they become, the more hazardous they are. Employees are exposed to a greater health hazard when machining thermally modified wood because a considerable amount of fine dust is produced under the same processing conditions than in the case of unmodified wood. The International Agency for Research on Cancer (IARC) states that wood dust causes cancer of the nasal cavity and paranasal sinuses and of the nasopharynx. Wood dust is also associated with toxic effects, irritation of the eyes, nose and throat, dermatitis, and respiratory system effects which include decreased lung capacity, chronic obstructive pulmonary disease, asthma and allergic reactions. In our research, granular composition of particles resulting from the process of longitudinal milling of heat-treated oak and spruce wood under variable conditions (i.e., the temperature of modification of 160, 180, 200 and 220 °C and feed rate of 6, 10 and 15 m.min−1) are presented in the paper. Sieve analysis was used to determine the granular composition of particles. An increase in fine particle fraction when the temperature of modification rises was confirmed by the research. This can be due to the lower strength of thermally modified wood. Moreover, a different effect of the temperature modification on granularity due to the tree species was observed. In the case of oak wood, changes occurred at a temperature of 160 °C and in the case of spruce wood, changes occurred at the temperatures of 200 and 220 °C. At the temperatures of modification of 200 and 220 °C, the dust fraction (i.e., that occurred in the mesh sieves, particles with the size ≤ 0.08 mm) ranged from 2.99% (oak wood, feed rate of 10 m.min−1) to 8.07% (spruce wood, feed rate of 6 m.min−1). Such particles might have a harmful effect on employee health in wood-processing facilities.
PbMYB120 Negatively Regulates Anthocyanin Accumulation in Pear
Authors: Song Linyan , Wang Xiaoli , Han Wei , Qu Yingying , Wang Zhigang , Zhai Rui , Yang Chengquan , Ma Fengwang , Xu Lingfei
Published: February 2020

Subgroup 4 R2R3 MYBs play vital roles in the regulation of anthocyanin biosynthesis. However, there is limited knowledge regarding the functions of MYB repressors in pear (Pyrus × bretschneideri). Here, PbMYB120 was identified as a potential regulator of anthocyanin biosynthesis. A phylogenetic analysis revealed that PbMYB120 was clustered into the FaMYB1-like clade of the subgroup 4 R2R3 MYBs. PbMYB120 was expressed higher in red peels than in green peels in five pear cultivars. PbMYB120 expression was positively correlated with anthocyanin accumulation. However, the transient overexpression of PbMYB120 led to the inhibition of anthocyanin accumulation and PbUFGT1 expression. Promoter binding and activation assays indicated that PbMYB120 binds to the promoter of PbUFGT1 and represses the promoter’s activity. Thus, the inhibition of anthocyanin accumulation by PbMYB120 may be correlated with the repression of PbUFGT1. Furthermore, during anthocyanin induction, the expression levels of anthocyanin activators and PbMYB120 were upregulated. This study demonstrated that PbMYB120 was highly expressed in pear tissues having higher anthocyanin accumulations but acted as a repressor in the regulation of anthocyanin accumulation. PbMYB120 may work coordinately with anthocyanin activators and serve as a balancer of anthocyanin accumulation.
Purification of Boron Nitride Nanotubes Enhances Biological Application Properties
Authors: Lee Soul-Hee , Kim Myung Jong , Ahn Seokhoon , Koh Byumseok
Published: February 2020

Commercially available boron nitride nanotubes (BNNTs) and their purified form (pBNNTs) were dispersed in aqueous solutions with various dispersants, and their cytotoxicity and drug encapsulation capacity were monitored. Our data suggest that pBNNTs showed an average increase in dispersibility of 37.3% in aqueous solution in the presence of 10 different dispersants. In addition, 100 μg of pBNNTs induced an average decrease in cytotoxicity of 27.4% compared to same amount of BNNTs in normal cell lines. The same amount of pBNNTs can encapsulate 10.4-fold more drug (camptothecin) compared to BNNTs. These data suggest that the purification of BNNTs improves several of their properties, which can be applied to biological experiments and are thus essential in the biological application of BNNTs.
Emerging Potential of Exosomes in Regenerative Medicine for Temporomandibular Joint Osteoarthritis
Authors: Lee Yeon-Hee , Park Hee-Kyung , Auh Q-Schick , Nah Haram , Lee Jae Seo , Moon Ho-Jin , Heo Dong Nyoung , Kim In San , Kwon Il Keun
Published: February 2020

Exosomes are nanosized vesicles (30–140 nm) of endocytic origin that play important roles in regenerative medicine. They are derived from cell membranes during endocytic internalization and stabilize in biological fluids such as blood and synovia. Temporomandibular joint osteoarthritis (TMJ OA) is a degenerative disease, which, in addition to chronic pain, is characterized by progressive cartilage breakdown, condylar bone remodeling, and synovitis. However, traditional clinical treatments have limited symptom- and structure-modifying effects to restore damaged cartilage and other TMJ tissues. This is due to the limited self-healing capacity of condylar cartilage. Recently, stem-cell-derived exosomes have been studied as an alternative therapeutic approach to tissue repair and regeneration. It is known that trophic regulation of mesenchymal stem cells (MSCs) has anti-inflammatory and immunomodulatory effects under pathological conditions, and research on MSC-derived exosomes is rapidly accumulating. MSC-derived exosomes mimic the major therapeutic effects of MSCs. They affect the activity of immune effector cells and possess multilineage differentiation potential, including chondrogenic and osteogenic differentiation. Furthermore, exosomes are capable of regenerating cartilage or osseous compartments and restoring injured tissues and can treat dysfunction and pain caused by TMJ OA. In this review, we looked at the uniqueness of TMJ, the pathogenesis of TMJ OA, and the potential role of MSC-derived exosomes for TMJ cartilage and bone regeneration.
A High Resolution Melting Analysis-Based Genotyping Toolkit for the Peach (Prunus persica) Chilling Requirement
Authors: Chou Lin , Huang Shih-Jie , Hsieh Chen , Lu Ming-Te , Song Chia-Wei , Hsu Fu-Chiun
Published: February 2020

The chilling requirement (CR) is the main factor controlling the peach floral bud break and subsequent reproductive growth. To date, several peach CR quantitative trait loci (QTLs) have been identified. To improve the accessibility and convenience of this genetic information for peach breeders, the aim of this study was to establish an easy-to-use genotype screening system using peach CR molecular markers as a toolkit for marker-assisted selection. Here, we integrated 22 CR-associated markers from three published QTLs and positioned them on the Prunus persica physical map. Then, we built a PCR-based genotyping platform by using high-resolution melting (HRM) analysis with specific primers and trained this platform with 27 peach cultivars. Due to ambiguous variant calls from a commercial HRM software, we developed an R-based pipeline using principal component analysis (PCA) to accurately differentiate genotypes. Based on the PCA results, this toolkit was able to determine the genotypes at the CR-related single nucleotide polymorphisms (SNPs) in all tested peach cultivars. In this study, we showed that this HRM-PCA pipeline served as a low-cost, high-throughput, and non-gel genotyping solution. This system has great potential to accelerate CR-focused peach breeding.
Analysis and Identification of Tumorigenic Targets of MicroRNA in Cancer Cells by Photoreactive Chemical Probes
Authors: Su Zhiyu , Ganbold Tsogzolmaa , Baigude Huricha
Published: February 2020

Photoactive RNA probes have unique advantages in the identification of microRNA (miR) targets due to their ability for efficient conjugation to the target sequences by covalent crosslinking, providing stable miR-mRNA complexes for further analysis. Here, we report a highly efficient and straightforward method for miR target identification that is based on photo-reactive chemical probes and RNA-seq technology (denotes PCP-Seq). UV reactive probes were prepared by incorporating psoralen in the specific position of the seed sequence of miR. Cancer cells that were transfected with the miR probes were treated with UV, following the isolation of poly(A) RNA and sequencing of the transcriptome. Quantitative analysis of RNA-seq reads and subsequent validation by qPCR, dual luciferase assay as well as western blotting confirmed that PCP-Seq could highly efficiently identify multiple targets of different miRs in the lung cancer cell line, such as targets PTTG1 and PTGR1 of miR-29a and ILF2 of miR-34a. Collectively, our data showed that PCP-Seq is a robust strategy for miR targets identification, and unique in the identification of the targets that escape degradation by miRISC and maintain normal cellular level, although their translation is repressed.
The Effects of Essential Oils and Terpenes in Relation to Their Routes of Intake and Application
Authors: Koyama Sachiko , Heinbockel Thomas
Published: February 2020

Essential oils have been used in multiple ways, i.e., inhaling, topically applying on the skin, and drinking. Thus, there are three major routes of intake or application involved: the olfactory system, the skin, and the gastro-intestinal system. Understanding these routes is important for clarifying the mechanisms of action of essential oils. Here we summarize the three systems involved, and the effects of essential oils and their constituents at the cellular and systems level. Many factors affect the rate of uptake of each chemical constituent included in essential oils. It is important to determine how much of each constituent is included in an essential oil and to use single chemical compounds to precisely test their effects. Studies have shown synergistic influences of the constituents, which affect the mechanisms of action of the essential oil constituents. For the skin and digestive system, the chemical components of essential oils can directly activate gamma aminobutyric acid (GABA) receptors and transient receptor potential channels (TRP) channels, whereas in the olfactory system, chemical components activate olfactory receptors. Here, GABA receptors and TRP channels could play a role, mostly when the signals are transferred to the olfactory bulb and the brain.
Nest Turrets of Acromyrmex Grass-Cutting Ants: Micromorphology Reveals Building Techniques and Construction Dynamics
Authors: Cosarinsky Marcela I. , Römer Daniela , Roces Flavio
Published: February 2020

Acromyrmex fracticornis grass-cutting ants construct conspicuous chimney-shaped nest turrets made of intermeshed grass fragments. We asked whether turrets are constructed by merely piling up nearby materials around the entrance, or whether ants incorporate different materials as the turret develops. By removing the original nest turrets and following their rebuilding process over three consecutive days, age-dependent changes in wall morphology and inner lining fabrics were characterized. Micromorphological descriptions based on thin sections of turret walls revealed the building behaviors involved. Ants started by collecting nearby twigs and dry grass fragments that are piled up around the nest entrance. Several large fragments held the structure like beams. As a net-like structure grew, soil pellets were placed in between the intermeshed plant fragments from the turret base to the top, reinforcing the structure. Concomitantly, the turret inner wall was lined with soil pellets, starting from the base. Therefore, the consolidation of the turret occurred both over time and from its base upwards. It is argued that nest turrets do not simply arise by the arbitrary deposition of nearby materials, and that workers selectively incorporate large materials at the beginning, and respond to the developing structure by reinforcing the intermeshed plant fragments over time.
Evaluating the Performance of the WHO International Reference Standard for Osteoporosis Diagnosis in Postmenopausal Women of Varied Polygenic Score and Race
Authors: Wu Qing , Xiao Xiangxue , Xu Yingke
Published: February 2020

Background: Whether the bone mineral density (BMD) T-score performs differently in osteoporosis classification in women of different genetic profiling and race background remains unclear. Methods: The genomic data in the Women’s Health Initiative study was analyzed (n = 2417). The polygenic score (PGS) was calculated from 63 BMD-associated single nucleotide polymorphisms (SNPs) for each participant. The World Health Organization′s (WHO) definition of osteoporosis (BMD T-score ≤ −2.5) was used to estimate the cumulative incidence of fracture. Results: T-score classification significantly underestimated the risk of major osteoporotic fracture (MOF) in the WHI study. An enormous underestimation was observed in African American women (POR: 0.52, 95% CI: 0.30–0.83) and in women with low PGS (predicted/observed ratio [POR]: 0.43, 95% CI: 0.28–0.64). Compared to Caucasian women, African American, African Indian, and Hispanic women respectively had a 59%, 41%, and 55% lower hazard of MOF after the T-score was adjusted for. The results were similar when used for any fractures. Conclusions: Our study suggested the BMD T-score performance varies significantly by race in postmenopausal women.
The Complex Balance between Analgesic Efficacy, Change of Dose and Safety Profile Over Time, in Cancer Patients Treated with Opioids: Providing the Clinicians with an Evaluation Tool
Authors: Corli Oscar , Porcu Luca , Santucci Claudia , Bosetti Cristina
Published: February 2020

Background: Scanty data exist on the integration between the analgesic effect of opioids, dose changes, and adverse events in cancer patients. Methods: To provide further information on this issue, we analysed data on 498 advanced-stage cancer patients treated with strong opioids. At baseline and three visits (at days 7, 14, and 21), pain intensity, oral morphine-equivalent daily dose, and the prevalence of major adverse events were measured. The proportion of responders (pain intensity decrease ≥30% from baseline) and non-responders, as well as of patients with low or high dose escalation, was calculated. Results: Pain intensity strongly decreased from baseline (pain intensity difference −4.0 at day 7 and −4.2 at day 21) in responders, while it was quite stable in non-responders (pain intensity difference −0.8 at day 7 and −0.9 at day 21). In low dose escalation patients (82.4% at final visit), daily dose changed from 52.3 to 65.3 mg; in high dose escalation patients (17.6%), it varied from 94.1 to 146.7 mg. Among responders, high dose escalation patients experienced significantly more frequent adverse events compared to low or high dose escalation patients, while no differences were observed in non-responders. Conclusions: The response to opioids results from the combination of three clinical aspects, which are strongly interrelated. These results provide some thoughts to help clinical evaluations and therapeutic decisions regarding opioid use.
Effect of rhTSH on Lipids
Authors: Delitala Alessandro P. , Scuteri Angelo , Maioli Margherita , Casu Gavino , Merella Pierluigi , Fanciulli Giuseppe
Published: February 2020

Background: Subclinical hypothyroidism is associated with increased blood lipid levels. However, the exact role of thyrotropin (TSH) alone is not clear. In order to clarify this point, we analysed the acute effect of recombinant human TSH (rhTSH) administration on lipid levels. Methods: Sera of 27 premenopausal women with well-differentiated thyroid cancer were analysed. Patients that underwent a total thyroidectomy, ablation with 131I (Iodine 131) and rhTSH administration as a part of routine follow-up American Thyroid Association guidelines were included. The protocol consists of 2 intramuscular injections of 0.9 mg of rhTSH, performed on day 1 day and day 2, with blood collection on day 1 (before rhTSH administration), and day 5. TSH, free thyroxine, total cholesterol, low-density lipoprotein cholesterol (LDLc), high density lipoprotein cholesterol (HDLc), and triglycerides were assessed in all the samples, before and four days after the first administration of rhTSH. Results: Total cholesterol and triglycerides significantly increased after stimulation of rhTSH (respectively, 192 ± 33 vs. 207 ± 26, p = 0.036 and 72 ± 23 vs. 85 ± 23, p = 0.016). LDLc and HDLc showed comparable concentrations before and after the test (respectively, 115 ± 27 vs. 126 ± 22, p = 0.066, and 62 ± 15 vs. 64 ± 15, p = 0.339), while non-HDLc increased after stimulation (130 ± 30 vs. 143 ± 25, p = 0.045). Conclusion: TSH has a direct effect on total cholesterol, triglycerides, and nonHDLc. Explanation of these phenomena will require additional studies.
Altered Gut Microbial Fermentation and Colonization with Methanobrevibacter smithii in Renal Transplant Recipients
Authors: Knobbe Tim J. , Douwes Rianne M. , Kremer Daan , Swarte J. Casper , Eisenga Michele F. , Gomes-Neto António W. , van Londen Marco , Peters Frans T. M. , Blokzijl Hans , Nolte Ilja M. , Hendriks Wouter H. , Harmsen Hermie J. M. , Bakker Stephan J. L.
Published: February 2020

Renal transplant recipients (RTRs) often suffer from posttransplant diarrhea. The observed dysbiosis in RTR may influence the fermentation processes in the gut. In this study, we aimed to investigate whether fermentation differs between RTRs and healthy controls (HCs), by measuring breath H2 and CH4 concentrations. Additionally, we determined the fecal presence of the methanogen Methanobrevibacter smithii (M. smithii), which plays a main role in the process of methanogenesis. Data from the TransplantLines Biobank and Cohort Study (NCT03272841) was used. A total of 142 RTRs and 77 HCs were included. Breath H2 concentrations in RTRs were not significantly different from HCs. Breath CH4 concentrations in RTRs were significantly lower compared with HCs (median [interquartile range (IQR)] 7.5 [3.9–10.6] ppm vs. 16.0 [8.0–45.5] ppm, p < 0.001). M. smithii was less frequently present in the feces of RTRs compared to HCs (28.6% vs. 86.4% resp., p < 0.001). Our findings regarding the altered methanogenesis in the gut of RTRs show similarities with previous results in inflammatory bowel disease patients. These findings provide novel insight into the alterations of fermentation after renal transplantation, which may contribute to understanding the occurrence of posttransplant diarrhea.
Impact of Lung Compliance on Neurological Outcome in Patients with Acute Respiratory Distress Syndrome Following Out-of-Hospital Cardiac Arrest
Authors: Kim June-sung , Kim Youn-Jung , Kim Muyeol , Ryoo Seung Mok , Sohn Chang Hwan , Ahn Shin , Kim Won Young
Published: February 2020

(1) Background: Acute respiratory distress syndrome (ARDS) following cardiac arrest is common and associated with in-hospital mortality. We aim to investigate whether lung compliance during targeted temperature management is associated with neurological outcome in patients with ARDS after out-of-hospital cardiac arrest (OHCA). (2) Methods: This observational study is conducted in the emergency intensive care unit from January 2011 to April 2019 using data from a prospective patient registry. Adult patients (age ≥18 years) who survived non-traumatic OHCA and subsequently developed ARDS based on the Berlin definition are included. Mechanical ventilator parameters such as plateau pressure, tidal volume, minute ventilation, positive end expiratory pressure, and compliance are recorded for 7 days or until death, and categorized as maximum, median, and minimum. The primary outcome is a favorable neurological outcome defined as a Cerebral Performance Category score of 1 or 2 at hospital discharge. (3) Results: Regarding 246 OHCA survivors, 119 (48.4%) patients developed ARDS. A favorable neurologic outcome was observed in 23 (19.3%). Patients with a favorable outcome have a significantly higher lung compliance (38.6 mL/cm H2O versus 27.5 mL/cm H2O), lower inspiratory pressure (12.0 cm H2O versus 16.0 cm H2O), and lower plateau pressure (17.0 cm H2O versus 21.0 cm H2O) than those with a poor neurologic outcome (all p < 0.01). Concerning time-dependent cox regression models, all maximum (adjusted hazard ratio [HR] 1.05, 95% confidence interval [CI] 1.02–1.09), minimum (HR 1.08, 95% CI 1.03–1.13), and median (HR 1.06, 95% CI 1.02–1.09) compliances are independently associated with a good neurologic outcome. Maximum compliance, >32.5 mL/cm H2O at day 1, has the highest area under the receiver operating characteristic curve (0.745) with a positive predictive value of 90.4%. (4) Conclusions: Lung compliance may be an early predictor of intact neurologic survival in patients with ARDS following cardiac arrest.
Modulation of SERCA in Patients with Persistent Atrial Fibrillation Treated by Epicardial Thoracoscopic Ablation: The CAMAF Study
Authors: Sardu Celestino , Santulli Gaetano , Guerra Germano , Trotta Maria Consiglia , Santamaria Matteo , Sacra Cosimo , Testa Nicola , Ducceschi Valentino , Gatta Gianluca , D' Amico Michele , Sasso Ferdinando Carlo , Paolisso Giuseppe , Marfella Raffaele
Published: February 2020

Objectives: To evaluate atrial fibrillation (AF) recurrence and Sarcoplasmic Endoplasmic Reticulum Calcium ATPase (SERCA) levels in patients treated by epicardial thoracoscopic ablation for persistent AF. Background: Reduced levels of SERCA have been reported in the peripheral blood cells of patients with AF. We hypothesize that SERCA levels can predict the response to epicardial ablation. Methods: We designed a prospective, multicenter, observational study to recruit, from October 2014 to June 2016, patients with persistent AF receiving an epicardial thoracoscopic pulmonary vein isolation. Results: We enrolled 27 patients. Responders (n = 15) did not present AF recurrence after epicardial ablation at one-year follow-up; these patients displayed a marked remodeling of the left atrium, with a significant reduction of inflammatory cytokines, B type natriuretic peptide (BNP), and increased levels of SERCA compared to baseline and to nonresponders (p < 0.05). Furthermore, mean AF duration (Heart rate (HR) 1.235 (1.037–1.471), p < 0.05), Left atrium volume (LAV) (HR 1.755 (1.126–2.738), p < 0.05), BNP (HR 1.945 (1.895–1.999), p < 0.05), and SERCA (HR 1.763 (1.167–2.663), p < 0.05) were predictive of AF recurrence. Conclusions: Our data indicate for the first time that baseline values of SERCA in patients with persistent AF might be predictive of failure to epicardial ablative approach. Intriguingly, epicardial ablation was associated with increased levels of SERCA in responders. Therefore, SERCA might be an innovative therapeutic target to improve the response to epicardial ablative treatments.
Quantitative Approach to Fragmented QRS in Arrhythmogenic Cardiomyopathy: From Disease towards Asymptomatic Carriers of Pathogenic Variants
Authors: Roudijk Rob W. , Bosman Laurens P. , van der Heijden Jeroen F. , de Bakker Jacques M. T. , Hauer Richard N. W. , van Tintelen J. Peter , Asselbergs Folkert W. , te Riele Anneline S. J. M. , Loh Peter
Published: February 2020

Fragmented QRS complexes (fQRS) are common in patients with arrhythmogenic cardiomyopathy (ACM). A new method of fQRS quantification may aid early disease detection in pathogenic variant carriers and assessment of prognosis in patients with early stage ACM. Patients with definite ACM (n = 221, 66%), carriers of a pathogenic ACM-associated variant without a definite ACM diagnosis (n = 57, 17%) and control subjects (n = 58, 17%) were included. Quantitative fQRS (Q-fQRS) was defined as the total amount of deflections in the QRS complex in all 12 electrocardiography (ECG) leads. Q-fQRS was scored by a single observer and reproducibility was determined by three independent observers. Q-fQRS count was feasible with acceptable intra- and inter-observer agreement. Q-fQRS count is significantly higher in patients with definite ACM (54 ± 15) and pathogenic variant carriers (55 ± 10) compared to controls (35 ± 5) (p < 0.001). In patients with ACM, Q-fQRS was not associated with sustained ventricular arrhythmia (p = 0.701) at baseline or during follow-up (p = 0.335). Both definite ACM patients and pathogenic variant carriers not fulfilling ACM diagnosis have a higher Q-fQRS than controls. This may indicate that increased Q-fQRS is an early sign of disease penetrance. In concealed and early stages of ACM the role of Q-fQRS for risk stratification is limited.