bluuebunny/crossref_metadata_2025 huggingface dataset:
Applicability of Anti-Human Estrogen Receptor β Antibody PPZ0506 for the Immunodetection of Rodent Estrogen Receptor β Proteins
Authors: Ishii Hirotaka , Otsuka Mai , Kanaya Moeko , Higo Shimpei , Hattori Yujiro , Ozawa Hitoshi
Published: December 2019

Several lines of controversial evidence concerning estrogen receptor β (ERβ) remain to be solved because of the unavailability of specific antibodies against ERβ. The recent validation analysis identified a monoclonal antibody (PPZ0506) with sufficient specificity against human ERβ. However, the specificity and cross-reactivity of PPZ0506 antibody against ERβ proteins from laboratory animals have not been confirmed. In the present study, we aimed to validate the applicability of PPZ0506 to rodent studies. The antibody exhibited specific cross-reactivity against mouse and rat ERβ proteins in immunoblot and immunocytochemical experiments using transfected cells. In immunohistochemistry for rat tissue sections, PPZ0506 showed immunoreactive signals in the ovary, prostate, and brain. These immunohistochemical profiles of rat ERβ proteins in rat tissues accord well with its mRNA expression patterns. Although the antibody was reported to show the moderate signals in human testis, no immunoreactive signals were observed in rat testis. Subsequent RT-PCR analysis revealed that this species difference in ERβ expression resulted from different expression profiles related to the alternative promoter usage between humans and rats. In conclusion, we confirmed applicability of PPZ0506 for rodent ERβ studies, and our results provide a fundamental basis for further examination of ERβ functions.
Transthyretin Upregulates Long Non-Coding RNA MEG3 by Affecting PABPC1 in Diabetic Retinopathy
Authors: Fan Guangming , Gu Yu , Zhang Jiaojiao , Xin Yu , Shao Jun , Giampieri Francesca , Battino Maurizio
Published: December 2019

The aim of the study was to demonstrate how transthyretin (TTR) could affect long non-coding RNA (lncRNA) of maternally expressed gene 3 (MEG3) and play important roles in diabetic retinopathy (DR). A DR model in C57BL/6 mice was established after intraperitoneal injection of streptozotocin (STZ). After intravitreal injection with TTR pAAV vector, MEG3 short hairpin RNA (shRNA), scrambled shRNA, or MEG3, retinal imaging, retinal trypsin digestion, and fundus vascular permeability tests were performed. Cell counting kit-8 (CCK8), transwell, and Matrigel assays were employed to detect the proliferation and migration of human retinal microvascular endothelial cells (hRECs). The binding between long non-coding RNA of maternally expressed gene 3 (lncRNA-MEG3) and microRNA-223-3p (miR-223-3p) was observed by using luciferase reporter assays, while co-immunoprecipitation (co-IP) was employed to confirm the interaction between TTR and the target. In the DR mice model, retinal vascular leakage and angiogenesis were repressed by overexpressing TTR. In vitro, the added TTR promoted the level of lncRNA-MEG3 by interacting with poly (A) binding protein cytoplasmic 1 (PABPC1), and then repressed proliferation and angiogenesis of hRECs. In vivo, silencing or overexpressing lncRNA-MEG3 significantly affected retinal vascular phenotypes. Additionally, the interaction between lncRNA-MEG3 and miR-223-3p was confirmed, and silencing of miR-223-3p revealed similar effects on hRECs as overexpression of lncRNA-MEG3. In summary, in the DR environment, TTR might affect the lncRNA MEG3/miR-223-3p axis by the direct binding with PABPC1, and finally repress retinal vessel proliferation.
Salacia chinensis L. Stem Extract Exerts Antifibrotic Effects on Human Hepatic Stellate Cells through the Inhibition of the TGF-β1-Induced SMAD2/3 Signaling Pathway
Authors: Phaosri Mattareeyapar , Jantrapirom Salinee , Na Takuathung Mingkwan , Soonthornchareonnon Noppamas , Sireeratawong Seewaboon , Buacheen Pensiri , Pitchakarn Pornsiri , Nimlamool Wutigri , Potikanond Saranyapin
Published: December 2019

Salacia chinensis L. (SC) stems have been used as an ingredient in Thai traditional medicine for treating patients with hepatic fibrosis and liver cirrhosis. However, there is no scientific evidence supporting the antifibrotic effects of SC extract. Therefore, this study aimed to determine the antifibrotic activity of SC stem extract in human hepatic stellate cell-line called LX-2. We found that upon TGF-β1 stimulation, LX-2 cells transformed to a myofibroblast-like phenotype with a noticeable increase in α-SMA and collagen type I production. Interestingly, cells treated with SC extract significantly suppressed α-SMA and collagen type I production and reversed the myofibroblast-like characteristics back to normal. Additionally, TGF-β1 also influenced the development of fibrogenesis by upregulation of MMP-2, TIMP-1, and TIMP-2 and related cellular signaling, such as pSmad2/3, pErk1/2, and pJNK. Surprisingly, SC possesses antifibrotic activity through the suppression of TGF-β1-mediated production of collagen type 1, α-SMA, and the phosphorylation status of Smad2/3, Erk1/2, and JNK. Taken together, the present study provides accumulated information demonstrating the antifibrotic effects of SC stem extract and revealing its potential for development for hepatic fibrosis patients.
Management of Refeeding Syndrome in Medical Inpatients
Authors: Reber Emilie , Friedli Natalie , Vasiloglou Maria F. , Schuetz Philipp , Stanga Zeno
Published: December 2019

Refeeding syndrome (RFS) is the metabolic response to the switch from starvation to a fed state in the initial phase of nutritional therapy in patients who are severely malnourished or metabolically stressed due to severe illness. It is characterized by increased serum glucose, electrolyte disturbances (particularly hypophosphatemia, hypokalemia, and hypomagnesemia), vitamin depletion (especially vitamin B1 thiamine), fluid imbalance, and salt retention, with resulting impaired organ function and cardiac arrhythmias. The awareness of the medical and nursing staff is often too low in clinical practice, leading to under-diagnosis of this complication, which often has an unspecific clinical presentation. This review provides important insights into the RFS, practical recommendations for the management of RFS in the medical inpatient population (excluding eating disorders) based on consensus opinion and on current evidence from clinical studies, including risk stratification, prevention, diagnosis, and management and monitoring of nutritional and fluid therapy.
Automatic Shoreline Detection from Eight-Band VHR Satellite Imagery
Authors: Alicandro Maria , Baiocchi Valerio , Brigante Raffaella , Radicioni Fabio
Published: December 2019

Coastal erosion, which is naturally present in many areas of the world, can be significantly increased by factors such as the reduced transport of sediments as a result of hydraulic works carried out to minimize flooding. Erosion has a significant impact on both marine ecosystems and human activities; for this reason, several international projects have been developed to study monitoring techniques and propose operational methodologies. The increasing number of available high-resolution satellite platforms (i.e., Copernicus Sentinel) and algorithms to treat them allows the study of original approaches for the monitoring of the land in general and for the study of the coastline in particular. The present project aims to define a methodology for identifying the instantaneous shoreline, through images acquired from the WorldView 2 satellite, on eight spectral bands, with a geometric resolution of 0.5 m for the panchromatic image and 1.8 m for the multispectral one. A pixel-based classification methodology is used to identify the various types of land cover and to make combinations between the eight available bands. The experiments were carried out on a coastal area with contrasting morphologies. The eight bands in which the images are taken produce good results both in the classification process and in the combination of the bands, through the algorithms of normalized difference vegetation index (NDVI), normalized difference water index (NDWI), spectral angle mapper (SAM), and matched filtering (MF), with regard to the identification of the various soil coverings and, in particular, the separation line between dry and wet sand. In addition, the real applicability of an algorithm that extracts bathymetry in shallow water using the “coastal blue” band was tested. These data refer to the instantaneous shoreline and could be corrected in the future with morphological and tidal data of the coastal areas under study.
Assessing Riyadh’s Urban Change Utilizing High-Resolution Imagery
Authors: Alghamdi Ali , Cummings Anthony R.
Published: December 2019

The implications of change on local processes have attracted significant research interest in recent times. In urban settings, green spaces and forests have attracted much attention. Here, we present an assessment of change within the predominantly desert Middle Eastern city of Riyadh, an understudied setting. We utilized high-resolution SPOT 5 data and two classification techniques—maximum likelihood classification and object-oriented classification—to study the changes in Riyadh between 2004 and 2014. Imagery classification was completed with training data obtained from the SPOT 5 dataset, and an accuracy assessment was completed through a combination of field surveys and an application developed in ESRI Survey 123 tool. The Survey 123 tool allowed residents of Riyadh to present their views on land cover for the 2004 and 2014 imagery. Our analysis showed that soil or ‘desert’ areas were converted to roads and buildings to accommodate for Riyadh’s rapidly growing population. The object-oriented classifier provided higher overall accuracy than the maximum likelihood classifier (74.71% and 73.79% vs. 92.36% and 90.77% for 2004 and 2014). Our work provides insights into the changes within a desert environment and establishes a foundation for understanding change in this understudied setting.
Effect of Sodium Hexametaphosphate and Trisodium Phosphate on Dispersion of Polycarboxylate Superplasticizer
Authors: Zhang Yan , Liu Huaqing , Liu Jialong , Tong Ruiming
Published: December 2019

Enhancement in dispersion of polycarboxylate superplasticizer (PCE) could be obtained by incorporating retarders in normal concrete. The generally believed reason was that the consumption of free water and polymer at the beginning was reduced by retarding cement hydration. This theory could not convincingly explain why sodium hexametaphosphate (SHMP) was able to promote the dispersion capacity of PCE, while trisodium phosphate (TSP) could not, despite that both TSP and SHMP could obviously retard the cement hydration. The adsorption behavior of PCE and phosphate was investigated and the mechanism was analyzed in order to gain deeper understanding. The results showed that TSP and SHMP delayed the cement hydration, impeded adsorption process of PCE, and increased thickness of adsorption layer. It was interesting that TSP reduced the dispersion, but SHMP enhanced. The reason for this contradiction was due to the difference in composition of adsorption layer. In the PCE-TSP system, this layer was composed of the precipitates (formed by TSP and Ca2+) and the invalided PCE (caused by these precipitates in the immediate vicinity of the cement grains); the invalided PCE was due to the decrease of PCE dispersion. In the PCE-SHMP system, “Inner-phosphate (multi-layers) + Outer-PCE (single layer)” structure was formed to make the PCE work more effective, hence enhancing the dispersion. These results were expected to be useful for the design of highly efficient dispersants.
Rapidly Solidified Aluminium Alloy Composite with Nickel Prepared by Powder Metallurgy: Microstructure and Self-Healing Behaviour
Authors: Michalcová Alena , Knaislová Anna , Kubásek Jiří , Kačenka Zdeněk , Novák Pavel
Published: December 2019

Composite material prepared by spark plasma sintering (SPS) from a powder mixture of AlCrFeSi rapidly solidified alloy and 5 wt. % of Ni particles was studied in this work. It was proven that during SPS compaction at 500 °C, no intermetallic phases formed on the surface of Ni particles. The material exhibited sufficient mechanical properties obtained by tensile testing (ultimate tensile stress of 203 ± 4 MPa, ductility of 0.8% and 0.2% offset yield strength of 156 ± 2 MPa). Tensile samples were pre-stressed to 180 MPa and annealed at 450 and 550 °C for 1 h. Annealing at 450 °C did not lead to any recovery of the material. Annealing at 550 °C caused the full recovery of 0.2% offset yield strength, while the ductility was decreased. The self-healing behaviour originates from the growth of intermetallic phases between the Ni particle and the Al matrix. The sequence of NiAl, Ni2Al3 and NiAl3 intermetallic phases formation was observed. In particular, the morphology of the NiAl3 phase, growing in thin dendrites into the Al matrix, is suitable for the closing of cracks, which pass through the material.
Classifying Evolution Algebras of Dimensions Two and Three
Authors: Celorrio M. Eugenia , Velasco M. Victoria
Published: December 2019

We classified evolution algebras of dimensions two and three. Evolution algebras of dimensions three were classified recently obtaining 116 non-isomorphic types of algebras. Herein, with a new approach, we classify these algebras into 14 non-isomorphic types of algebra, so that this new classification is easier to deal with.
Study on a High Performance MEMS Infrared Thermopile Detector
Authors: Bao Aida , Lei Cheng , Mao Haiyang , Li Ruirui , Guan Yihao
Published: December 2019

This paper presents a high-performance micro-electromechanical systems (MEMS) thermopile infrared detector. It consists of a double-end beam and a dual-layer thermocouple structure, which improves the responsivity of the detector. The etch-stop structure is integrated into the detector to prevent isotropic etching-caused damage on the device. The responsivity of the detector achieved 1151.14 V/W, and the measured response time was 14.46 ms. The detector had the potential to work as a high-precision temperature sensor and as a vacuum sensor.
Adaptive Nonlinearity Compensation System for Integrated Temperature and Moisture Sensor
Authors: Chen Guohong , Zhou Shengjun , Ni Jie , Huang Hao
Published: December 2019

Measuring temperature and moisture are important in many scenarios. It has been verified that temperature greatly affects the accuracy of moisture sensing. Moisture sensing performance would suffer without temperature calibrations. This paper introduces a nonlinearity compensation technique for temperature-dependent nonlinearity calibration of moisture sensors, which is based on an adaptive nonlinear order regulating model. An adaptive algorithm is designed to automatically find the optimal order number, which was subsequently applied in a nonlinear mathematical model to compensate for the temperature effects and improve the moisture measurement accuracy. The integrated temperature and moisture sensor with the proposed adaptive nonlinear order regulating nonlinearity compensation technique is found to be more effective and yield better sensing performance.
The Gut Microbiota in Cardiovascular Disease and Arterial Thrombosis
Authors: Lässiger-Herfurth Anna , Pontarollo Giulia , Grill Alexandra , Reinhardt Christoph
Published: December 2019

The gut microbiota has emerged as a contributing factor in the development of atherosclerosis and arterial thrombosis. Metabolites from the gut microbiota, such as trimethylamine N-oxide and short chain fatty acids, were identified as messengers that induce cell type-specific signaling mechanisms and immune reactions in the host vasculature, impacting the development of cardiovascular diseases. In addition, microbial-associated molecular patterns drive atherogenesis and the microbiota was recently demonstrated to promote arterial thrombosis through Toll-like receptor signaling. Furthermore, by the use of germ-free mouse models, the presence of a gut microbiota was shown to influence the synthesis of endothelial adhesion molecules. Hence, the gut microbiota is increasingly being recognized as an influencing factor of arterial thrombosis and attempts of dietary pre- or probiotic modulation of the commensal microbiota, to reduce cardiovascular risk, are becoming increasingly significant.
Protective or Detrimental? Understanding the Role of Host Immunity in Leishmaniasis
Authors: dos Santos Meira Camila , Gedamu Lashitew
Published: December 2019

The intracellular protozoan parasites of the genus Leishmania are the causative agents of leishmaniasis, a vector-borne disease of major public health concern, estimated to affect 12 million people worldwide. The clinical manifestations of leishmaniasis are highly variable and can range from self-healing localized cutaneous lesions to life-threatening disseminated visceral disease. Once introduced into the skin by infected sandflies, Leishmania parasites interact with a variety of immune cells, such as neutrophils, monocytes, dendritic cells (DCs), and macrophages. The resolution of infection requires a finely tuned interplay between innate and adaptive immune cells, culminating with the activation of microbicidal functions and parasite clearance within host cells. However, several factors derived from the host, insect vector, and Leishmania spp., including the presence of a double-stranded RNA virus (LRV), can modulate the host immunity and influence the disease outcome. In this review, we discuss the immune mechanisms underlying the main forms of leishmaniasis, some of the factors involved with the establishment of infection and disease severity, and potential approaches for vaccine and drug development focused on host immunity.
Surface Properties and Floatability Comparison of Aegirite and Specularite by Density Functional Theory Study and Experiment
Authors: Li Mingyang , Liu Jun , Gao Xiangpeng , Hu Yiming , Tong Xiong , Zhao Fugang , Yuan Qidong
Published: December 2019

Understanding the differences in surface properties between aegirite and specularite is of great significance to study their separation. In this work, the surface properties of aegirite and specularite, as well as their relationships to floatability, have been explored by first principle calculation, flotation, and Zeta potential measurement. The surface relaxation indicated that the specularite (001) surface appeared to show more surface reconstruction. The unsatisfied bond properties, Mulliken bond population, and surface charge showed that the floatability of specularite was superior to that of aegirite. The flotation results showed that the hydrophobicity of specularite was higher than that of aegirite with dodecylamine (DDA) as the collector. It is infeasible to separate specularite from aegirite by flotation using starch as the depressant, and research of effective reagents with high affinity to the element Si is the subclinical breakthrough point of specularite/aegirite separation.
Risk Assessment of Contamination by Potentially Toxic Metals: A Case Study in the Vicinity of an Abandoned Pyrite Mine
Authors: Ma Tingting , Zhou Wei , Yang Xiao , Christie Peter , Luo Yongming
Published: December 2019

Abandoned mining areas can display soil and water pollution and also a high incidence of endemic diseases. Here, based on preliminary results on mental retardation and high incidence rates of cancers, we investigate the contamination status and potential ecological risk at an abandoned pyrite mine located in Xianju village, Hubei province, central China. The study focused on the three potentially toxic metals As, Pb, and Zn and four other common elements, including Ca, Fe, S, and Se. Soil samples were collected from 12 sites and leachate seeping from five sites. Leachates were strongly acidic compared to the soil, with pH values <3.22. Soil As and Se concentrations exceeded the Chinese Grade II standards for soil environmental quality (GB 15618-2009) (30 and 3 mg·kg−1, respectively), ranging from 33.6 ± 0.7 to 78.2 ± 1.0 mg As·kg−1 dry matter (DM) and 5.3 ± 0.8 to 17.1 ± 1.4 mg Se·kg−1 DM. Arsenic, Fe, Se, and S in leachates all exceeded the Chinese Grade III standard for surface water environmental quality (GB 3838-2002) (0.1, 0.3, 0.03, and 1.0 mg·L−1). The potential ecological risks from Pb and Zn in soils were low, but As in soils and leachates represented a moderate or strong risk to children according to the Nemerow index and hazard quotient. Soil replacement combined with further remediation measures is required to remediate the contaminated area.
Dewatering of Mine Tailings Slurries Using Superabsorbent Polymers (SAPs) Reclaimed from Industrial Reject of Baby Diapers: A Preliminary Study
Authors: Sahi Amal , El Mahboub Khadija , Belem Tikou , Maqsoud Abdelkabir , Mbonimpa Mamert
Published: December 2019

Traditional deposition of tailings slurry in a tailings storage facility (TSF) can create risks of dike failure. In order to minimize these risks and slurry spillage, the surface deposition technique of densified tailings (DT) through dewatering of the slurry has emerged. The DT technique has the potential to maximize water reuse, improve the shear strength of surface tailings, and reduce the ecological footprint of TSF. The consistency of DT covers a continuum ranging from thickened state, to paste state, to dry state. Despite its efficiency and economic feasibility, DT densification using thickeners sometimes proves unable to achieve the design target solids mass concentration (Cw%). Hence, the use of superabsorbent polymers (SAPs) seems to represent a promising alternative, owing to their higher water absorbent capacity. In this paper, superabsorbent polymers (SAPs) reclaimed from industrial reject of baby diapers (Na-polyacrylates) are explored as a promising alternative to mine tailings slurries dewatering. To this end, laboratory-scale dewatering tests have been performed using two grades of Na-polyacrylate SAPs (grade 1 SAP = SAG-A06P coarse-grained, and grade 2 = SAG-M01P-100 medium-grained) for the tailings slurries densification. A higher water absorbency (or swelling capacity) was observed using the coarser SAPs (SAG-A06P) compared to the finer SAPs (SAG-M01P-100). The preliminary results showed that a SAP volume dosage in the range 10–13 kg of SAP/m3 of slurry allowed achieving a final solids mass concentration (Cw%_final) ≥ 70%, despite the occurrence of gel-blocking phenomenon.
Limitations of Recent Studies Dealing with the Antibacterial Properties of Silver Nanoparticles: Fact and Opinion
Authors: Duval Raphaël E. , Gouyau Jimmy , Lamouroux Emmanuel
Published: December 2019

Due to the constant increase in the number of infectious diseases and the concomitant lack of treatment available, metallic nanoparticles (e.g., silver nanoparticles) have been of particular interest in the last decades. Indeed, several studies suggest that silver nanoparticles have valuable antimicrobial activities, especially against bacteria, which may lead us to think that these nanoparticles may one day be an attractive therapeutic option for the treatment of bacterial infections. Unfortunately, when we look a little closer to these studies, we can see a very great heterogeneity (e.g., in the study design, in the synthetic process of nanoparticles, in the methods that explore the antibacterial properties of nanoparticles and in the bacteria chosen) making cross-interpretation between these studies impossible, and significantly limiting the interest of silver nanoparticles as promising antibacterial agents. We have selected forty-nine international publications published since 2015, and propose to discuss, not the results obtained, but precisely the different methodologies developed in these publications. Through this discussion, we highlighted the aspects to improve, or at least to homogenize, in order to definitively establish the interest of silver nanoparticles as valuable antibacterial agents.
Induction of Hair Keratins Expression by an Annurca Apple-Based Nutraceutical Formulation in Human Follicular Cells
Authors: Piccolo Marialuisa , Ferraro Maria Grazia , Maione Francesco , Maisto Maria , Stornaiuolo Mariano , Tenore Gian Carlo , Santamaria Rita , Irace Carlo , Novellino Ettore
Published: December 2019

Hair disorders may considerably impact the social and psychological well-being of an individual. Recent advances in the understanding the biology of hair have encouraged the research and development of novel and safer natural hair growth agents. In this context, we have previously demonstrated—at both preclinical and clinical level—that an Annurca apple-based dietary supplement (AMS), acting as a nutraceutical, is endowed with an intense hair-inductive activity (trichogenicity), at once increasing hair tropism and keratin content. Herein, in the framework of preclinical investigations, new experiments in primary human models of follicular keratinocytes and dermal papilla cells have been performed to give an insight around AMS biological effects on specific hair keratins expression. As well as confirming the biocompatibility and the antioxidant proprieties of our nutraceutical formulation, we have proven an engagement of trichokeratins production underlying its biological effects on human follicular cells. Annurca apples are particularly rich in oligomeric procyanidins, natural polyphenols belonging to the broader class of bioflavonoids believed to exert many beneficial health effects. To our knowledge, none of the current available remedies for hair loss has hitherto shown to stimulate the production of hair keratins so clearly.
First Trimester Maternal Vitamin D Status and Risks of Preterm Birth and Small-For-Gestational Age
Authors: Monier Isabelle , Baptiste Amandine , Tsatsaris Vassilis , Senat Marie-Victoire , Jani Jacques , Jouannic Jean-Marie , Winer Norbert , Elie Caroline , Souberbielle Jean-Claude , Zeitlin Jennifer , Benachi Alexandra
Published: December 2019

Maternal 25-hydroxyvitamin D (25-OHD) deficiency during pregnancy may increase the risk of preterm and small-for-gestational age (SGA) birth, but studies report conflicting results. We used a multicenter prospective cohort of 2813 pregnant women assessed for 25-OHD levels in the first trimester of pregnancy to investigate the association between maternal 25-OHD concentrations and risks of preterm birth (<37 weeks) and SGA (birthweight <10th percentile). Odds ratios were adjusted (aOR) for potential cofounders overall and among women with light and dark skin separately, based on the Fitzpatrick scale. 25-OHD concentrations were <20 ng/mL for 45.1% of the cohort. A total of 6.7% of women had a preterm birth. The aOR for preterm birth associated with the 1st quartile of 25-OHD concentrations compared to the 4th quartile was 1.53 (95% confidence interval (CI): 0.97–2.43). In stratified analyses, an association was observed for women with darker skin (aOR = 2.89 (95% CI: 1.02–8.18)), and no association with lighter skin. A total of 11.9% of births were SGA and there was no association overall or by skin color. Our results do not provide support for an association between maternal first trimester 25-OHD deficiency and risk of preterm or SGA birth overall; the association with preterm birth risk among women with darker skin requires further investigation.
Diet Quality and Micronutrient Intake among Long-Term Weight Loss Maintainers
Authors: Pascual Rebecca W. , Phelan Suzanne , La Frano Michael R. , Pilolla Kari D. , Griffiths Zoe , Foster Gary D.
Published: December 2019

Inadequate vitamin and mineral intake is documented among individuals with obesity, but is unknown among long-term weight loss maintainers (WLM). This study examined dietary quality and micronutrient adequacy among WLMs in a commercial weight management program. Participants were 1207 WLM in Weight Watchers (WW) who had maintained a 9.1 kg or greater weight loss (29.7 kg on average) for 3.4 years and had a body mass index (BMI) of 28.3 kg/m2. A control group of weight stable adults with obesity (controls; N = 102) had a BMI of 41.1 kg/m2. Measures included the Diet History Questionnaire-II, Healthy Eating Index-2015 (HEI), and Dietary References Intakes. WLM versus controls had a 10.1 point higher HEI score (70.2 (69.7–70.7) vs. 60.1 (58.4–61.8); p = 0.0001) and greater odds of meeting recommendations for copper (OR = 5.8 (2.6–13.1)), magnesium (OR = 2.9 (1.8–4.7)), potassium (OR = 4.7 (1.4–16.5)), vitamin A (OR = 2.8 (1.7–4.8)), vitamin B6 (OR = 2.9 (1.6–5.2)), and vitamin C (OR = 5.0 (2.8–8.8)). WLM, compared to controls, also reported higher percentages of calories from carbohydrates (50.3% (49.7–50.8) vs. 46.7% (44.8–48.7); p = 0.0001) and protein (18.2% (18.0–18.5) vs. 15.9% (15.1–16.6); p = 0.0001) and lower calories from fat (32.3% (31.9–32.8) vs. 37.4% (35.8–38.9); p = 0.0001). Long-term weight loss maintenance in a widely used commercial program was associated with a healthier diet pattern, including consuming foods with higher micronutrient density.
Non-Energy-Restricted Low-Carbohydrate Diet Combined with Exercise Intervention Improved Cardiometabolic Health in Overweight Chinese Females
Authors: Sun Shengyan , Kong Zhaowei , Shi Qingde , Hu Mingzhu , Zhang Haifeng , Zhang Di , Nie Jinlei
Published: December 2019

This study aimed to examine the effects of four weeks of a low-carbohydrate diet (LC) and incorporated exercise training on body composition and cardiometabolic health. Fifty-eight overweight/obese Chinese females (age: 21.2 ± 3.3 years, body mass index (BMI): 25.1 ± 2.8 kg/m2) were randomly assigned to the control group (CON, n = 15), the LC control group (LC-CON, n = 15), the LC and high-intensity interval training group (LC-HIIT, n = 15), or the LC and moderate-intensity continuous training group (LC-MICT, n = 13). Subjects consumed a four week LC, whereas LC-HIIT and LC-MICT received extra training 5 d/week (LC-HIIT: 10 × 6 s cycling interspersed with 9 s rest, MICT: 30 min continuous cycling at 50–60% VO2peak). After intervention, the three LC groups demonstrated significant reductions in body weight (−2.85 kg in LC-CON, −2.85 kg in LC-HIIT, −2.56 kg in LC-MICT, p < 0.001, η2 = 0.510), BMI (p < 0.001, η2 = 0.504) and waist-to-hip ratio (p < 0.001, η2 = 0.523). Groups with extra training (i.e., LC-HIIT and LC-MICT) improved VO2peak by 14.8 and 17.3%, respectively. However, fasting glucose and blood lipid levels remained unchanged in all groups. Short-term LC is a useful approach to improve body composition in overweight/obese Chinese females. Incorporated exercise training has no additional effects on weight loss, but has additional benefits on cardiorespiratory fitness, and HIIT is more time efficient than the traditional MICT (2.5 min vs. 30 min).
Water Quality as a Predictor of Legionella Positivity of Building Water Systems
Authors: Pierre David , Baron Julianne L. , Ma Xiao , Sidari Frank P. , Wagener Marilyn M. , Stout Janet E.
Published: December 2019

Testing drinking water systems for the presence of Legionella colonization is a proactive approach to assess and reduce the risk of Legionnaires’ disease. Previous studies suggest that there may be a link between Legionella positivity in the hot water return line or certain water quality parameters (temperature, free chlorine residual, etc.) with distal site Legionella positivity. It has been suggested that these measurements could be used as a surrogate for testing for Legionella in building water systems. We evaluated the relationship between hot water return line Legionella positivity and other water quality parameters and Legionella colonization in premise plumbing systems by testing 269 samples from domestic cold and hot water samples in 28 buildings. The hot water return line Legionella positivity and distal site positivity only demonstrated a 77.8% concordance rate. Hot water return line Legionella positivity compared to distal site positivity had a sensitivity of 55% and a specificity of 96%. There was poor correlation and a low positive predictive value between the hot water return line and distal outlet positivity. There was no correlation between Legionella distal site positivity and total bacteria (heterotrophic plate count), pH, free chlorine, calcium, magnesium, zinc, manganese, copper, temperature, total organic carbon, or incoming cold-water chlorine concentration. These findings suggest that hot water return line Legionella positivity and other water quality parameters are not predictive of distal site positivity and should not be used alone to determine the building’s Legionella colonization rate and effectiveness of water management programs.
The Effect of Abiotic Stress Conditions on Expression of Calmodulin (CaM) and Calmodulin-Like (CML) Genes in Wild-Growing Grapevine Vitis amurensis
Authors: Dubrovina Alexandra S. , Aleynova Olga A. , Ogneva Zlata V. , Suprun Andrey R. , Ananev Alexey A. , Kiselev Konstantin V.
Published: December 2019

Plant calmodulins (CaMs) and calmodulin-like proteins (CMLs) are important plant Ca2+-binding proteins that sense and decode changes in the intracellular Ca2+ concentration arising in response to environmental stimuli. Protein Ca2+ sensors are presented by complex gene families in plants and perform diverse biological functions. In this study, we cloned, sequenced, and characterized three CaM and 54 CML mRNA transcripts of Vitis amurensis Rupr., a wild-growing grapevine with a remarkable stress tolerance. Using real-time quantitative RT-PCR, we analyzed transcript abundance of the identified VaCaMs and VaCMLs in response to water deficit, high salinity, high mannitol, cold and heat stresses. Expression of VaCaMs and 32 VaCMLs actively responded to the abiotic stresses and exhibited both positive and negative regulation patterns. Other VaCML members showed slight transcriptional regulation, remained essentially unresponsive or responded only after one time interval of the treatments. The substantial alterations in the VaCaM and VaCML transcript levels revealed their involvement in the adaptation of wild-growing grapevine to environmental stresses.
Conjunctive Analyses of BSA-Seq and BSR-Seq to Reveal the Molecular Pathway of Leafy Head Formation in Chinese Cabbage
Authors: Li Rui , Hou Zhongle , Gao Liwei , Xiao Dong , Hou Xilin , Zhang Changwei , Yan Jiyong , Song Lixiao
Published: December 2019

As the storage organ of Chinese cabbage, the leafy head was harvested as a commercial product due to its edible value. In this study, the bulked segregant analysis (BSA) and bulked segregant RNA-Seq (BSR) were performed with F2 separation progeny to study the molecular mechanism of leafy head formation in Chinese cabbage. BSA-Seq analysis located four candidate regions containing 40 candidate genes, while BSR-Seq analysis revealed eight candidate regions containing 607 candidate genes. The conjunctive analyses of these two methods identified that Casein kinase gene BrCKL8 (Bra035974) is the common candidate gene related with leafy head formation in Chinese cabbage, and it showed high expression levels at the three segments of heading type plant leaves. The differentially expressed genes (DEGs) between two set pairs of cDNA sequencing bulks were divided into two categories: one category was related with five hormone pathways (Auxin, Ethylene, Abscisic acid, Jasmonic acid and Gibberellin), the other category was composed of genes that associate with the calcium signaling pathway. Moreover, a series of upregulated transcriptional factors (TFs) were also identified by the association analysis of BSR-Seq analysis. The leafy head development was regulated by various biological processes and effected by diverse external environment factors, so our research will contribute to the breeding of perfect leaf-heading types of Chinese cabbage.
Significantly Improved Electrical Properties of Photo-Initiated Auxiliary Crosslinking EPDM Used for Cable Termination
Authors: Li Zhong-Yuan , Sun Wei-Feng , Zhao Hong
Published: December 2019

In order to achieve high quality electrical materials for cable terminations, the crosslinked ethylene-propylene-diene monomer (EPDM) materials, with adequate breakdown strength, appropriately increased conductivity and are developed by employing auxiliary crosslinker and ultraviolet (UV) photoinitiated crosslinking technique. The characteristic cyclic anhydrides with coupled carbonyl groups are utilized as auxiliary crosslinkers to promote crosslinking efficiency and provide polar-groups to EPDM molecules in UV-initiated crosslinking processes, which can be effectively fulfilled in industrial cable production. The results of infrared spectroscopy show that the auxiliary crosslinkers have been successfully grated to EPDM molecules through UV initiation process. The conductivity of EPDM increases after individually utilizing three auxiliary crosslinkers to EPDM at various temperatures of cable operations, by which the highest conductivity has been acquired by grafting N.N-m-phenylene dimaleimide. The first-principles calculations demonstrate that some occupied local electronic-states have been introduced in the band-gap of the EPDM crosslinked by N.N-m-phenylene dimaleimide (EPDM-HAV2), which can be thermally excited from valence band to conduction band at lower temperature or in higher density, leading to augmentation in electrical conductivity. Meanwhile, the breakdown strength achieves a significant improvement in consistency with the theoretical estimation that deeper hole-traps can be introduced by auxiliary-crosslinking modification, and will consequently increase breakdown strength through the trapping mechanism of space charge suppression. in relation to the appropriately increased conductivity, in combination with persistent breakdown strength, the finite element simulations of the electric field distribution in EPDM cable terminations suggest that the effectively homogenized electric field at the root of stress cone will be realized for EPDM-HAV2. The present study offers a fundamental strategy to ameliorate EPDM materials in the application of insulated cable accessories.
Effect of Lignin Plasticization on Physico-Mechanical Properties of Lignin/Poly(Lactic Acid) Composites
Authors: Park Chan-Woo , Youe Won-Jae , Kim Seok-Ju , Han Song-Yi , Park Ji-Soo , Lee Eun-Ah , Kwon Gu-Joong , Kim Yong-Sik , Kim Nam-Hun , Lee Seung-Hwan
Published: December 2019

Kraft lignin (KL) or plasticized KL (PKL)/poly(lactic acid) (PLA) composites, containing different lignin contents and with and without the coupling agent, were prepared in this study using twin-screw extrusion at 180 °C. Furthermore, ε-caprolactone and polymeric diphenylmethane diisocyanate (pMDI) were used as a plasticizer of KL and a coupling agent to improve interfacial adhesion, respectively. It was found that lignin plasticization improved lignin dispersibility in the PLA matrix and increased the melt flow index due to decrease in melt viscosity. The tensile strength of KL or PKL/PLA composites was found to decrease as the content of KL and PKL increased in the absence of pMDI, and increased due to pMDI addition. The existence of KL and PKL in the composites decreased the thermal degradation rate against the temperature and increased char residue. Furthermore, the diffusion coefficient of water in the composites was also found to decrease due to KL or PKL addition.
Antibacterial Films Based on PVA and PVA–Chitosan Modified with Poly(Hexamethylene Guanidine)
Authors: Olewnik-Kruszkowska Ewa , Gierszewska Magdalena , Jakubowska Ewelina , Tarach Iwona , Sedlarik Vladimir , Pummerova Martina
Published: December 2019

In this study, thin, polymeric films consisting of poly(vinyl alcohol) (PVA) and chitosan (Ch) with the addition of poly(hexamethylene guanidine) (PHMG) were successfully prepared. The obtained materials were analyzed to determine their physicochemical and biocidal properties. In order to confirm the structure of PHMG, nuclear magnetic resonance spectroscopy (1H NMR) was applied, while in the case of the obtained films, attenuated total reflectance infrared spectroscopy with Fourier transform (FTIR-ATR) was used. The surface morphology of the polymer films was evaluated based on atomic force microscopy. Furthermore, the mechanical properties, color changes, and thermal stability of the obtained materials were determined. Microbiological tests were performed to evaluate the biocidal properties of the new materials with and without the addition of PHMG. These analyses confirmed the biocidal potential of films modified by PHMG and allowed for comparisons of their physicochemical properties with the properties of native films. In summary, films consisting of PVA and PHMG displayed higher antimicrobial potentials against Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli) bacteria in comparison to PVA:Ch-based films with the addition of PHMG.
The Copper(II) Ions Solvent Extraction with a New Compound: 2,6-Bis(4-Methoxybenzoyl)-Diaminopyridine
Authors: Bożejewicz Daria , Witt Katarzyna , Kaczorowska Małgorzata A. , Ośmiałowski Borys
Published: December 2019

A new compound 2,6-bis(4-methoxybenzoyl)-diaminopyridine (L) was used as an extractant for copper(II) ion recovery in a solvent extraction conducted at a temperature of 25 °C. The best results (99% recovery of copper(II) ions) were obtained when the aqueous phase contained 0.001 mol/dm3 Cu(II) and 0.2 mol/dm3 NH3 (pH~5.8), while the organic phase was a 0.001 mol/dm3 chloroform solution of 2,6-bis(4-methoxybenzoyl)-diaminopyridine. Spectrophotometry studies were used to determine the dissociation constant of the tested compound and determine the stability constant of the complex of subjected compound with copper(II) ions. The high-resolution mass spectrometry (HRMS) and higher energy collisional dissociation tandem mass spectrometry (HCD MS/MS) methods have been applied for the confirmation of the structure of 2,6-bis(4-methoxybenzoyl)-diaminopyridine and to determine its complexation with Cu(II) in solution.
Developing an Impairment Loss Given Default Model Using Weighted Logistic Regression Illustrated on a Secured Retail Bank Portfolio
Authors: Breed Douw Gerbrand , Verster Tanja , Schutte Willem D. , Siddiqi Naeem
Published: December 2019

This paper proposes a new method to model loss given default (LGD) for IFRS 9 purposes. We develop two models for the purposes of this paper—LGD1 and LGD2. The LGD1 model is applied to the non-default (performing) accounts and its empirical value based on a specified reference period using a lookup table. We also segment this across the most important variables to obtain a more granular estimate. The LGD2 model is applied to defaulted accounts and we estimate the model by means of an exposure weighted logistic regression. This newly developed LGD model is tested on a secured retail portfolio from a bank. We compare this weighted logistic regression (WLR) (under the assumption of independence) with generalised estimating equations (GEEs) to test the effects of disregarding the dependence among the repeated observations per account. When disregarding this dependence in the application of WLR, the standard errors of the parameter estimates are underestimated. However, the practical effect of this implementation in terms of model accuracy is found to be negligible. The main advantage of the newly developed methodology is the simplicity of this well-known approach, namely logistic regression of binned variables, resulting in a scorecard format.
Exposure of Marine Turtle Nesting Grounds to Named Storms Along the Continental USA
Authors: Fuentes Mariana M. P. B. , Godfrey Matthew H. , Shaver Donna , Ceriani Simona , Gredzens Christian , Boettcher Ruth , Ingram Dianne , Ware Matthew , Wildermann Natalie
Published: December 2019

Named storms can cause substantial impacts on the habitat and reproductive output of threatened species, such as marine turtles. To determine the impacts of named storms on marine turtles and inform management, it is necessary to determine the exposure of marine turtle nesting grounds to recent storm activities. To address this, remote sensing information of named storm tracks coupled with nesting ground data were used to investigate the temporal and spatial overlap between nesting grounds for four species of marine turtles in the continental United States of America. All species of marine turtles were exposed to named storms, with variation in exposure driven by the spatial distribution of each population’s nesting ground, the temporal overlap between the storms and reproductive events, and nest placement on the beach. Loggerhead turtles were the most exposed species to named storms, with the northern management unit having significantly higher exposure levels than all other loggerhead management units. Kemp’s ridley turtles, in contrast, were found to be the least exposed species to named storms. This study establishes a valuable current baseline against which to measure and compare future impacts that result as climate change progresses and storms become more frequent and intense. Importantly, cumulative and synergetic effects from other climatic processes and anthropogenic stressors should be considered in future analysis.
Ship Identification and Characterization in Sentinel-1 SAR Images with Multi-Task Deep Learning
Authors: Dechesne Clément , Lefèvre Sébastien , Vadaine Rodolphe , Hajduch Guillaume , Fablet Ronan
Published: December 2019

The monitoring and surveillance of maritime activities are critical issues in both military and civilian fields, including among others fisheries’ monitoring, maritime traffic surveillance, coastal and at-sea safety operations, and tactical situations. In operational contexts, ship detection and identification is traditionally performed by a human observer who identifies all kinds of ships from a visual analysis of remotely sensed images. Such a task is very time consuming and cannot be conducted at a very large scale, while Sentinel-1 SAR data now provide a regular and worldwide coverage. Meanwhile, with the emergence of GPUs, deep learning methods are now established as state-of-the-art solutions for computer vision, replacing human intervention in many contexts. They have been shown to be adapted for ship detection, most often with very high resolution SAR or optical imagery. In this paper, we go one step further and investigate a deep neural network for the joint classification and characterization of ships from SAR Sentinel-1 data. We benefit from the synergies between AIS (Automatic Identification System) and Sentinel-1 data to build significant training datasets. We design a multi-task neural network architecture composed of one joint convolutional network connected to three task specific networks, namely for ship detection, classification, and length estimation. The experimental assessment shows that our network provides promising results, with accurate classification and length performance (classification overall accuracy: 97.25%, mean length error: 4.65 m ± 8.55 m).