bluuebunny/crossref_metadata_2025 huggingface dataset:
Nanobiotechnological Approaches to Enhance Potato Resistance against Potato Leafroll Virus (PLRV) Using Glycyrrhizic Acid Ammonium Salt and Salicylic Acid Nanoparticles
Authors: Shoala Tahsin , Al-Karmalawy Ahmed , Germoush Mousa , ALshamrani Salha , Abdein Mohamed , Awad Nabil
Published: October 2021

The present research was aiming to study In-Silico the effect of Glycyrrhizic Acid ammonium salt (GAS) and Salicylic acid (SA) on the coat protein of potato leafroll virus (PLRV). In addition, in-vitro studying the effect of (GAS NPs) and Salicylic acid (SA NPs) nanoparticles at concentrations 0.15, 0.30, 1.25 and 2.5 mM, respectively, to control, decline or reduce the presence of PLRV in potato plants Solanum tuberosum L. selena. (GAS NPs) and (SA NPs) were applied in the MS medium at concentrations 0.15, 0.30, 1.25 and 2.5 mM, respectively. Results revealed that, enhancement or decline the PLRV according to the initiation of specific pathways. The expression level of Kinase 3 gene increased significantly due to the two used concentrations of GAS NPs. While the expression of callose gene was upregulated significantly in response to treatment of PLRV infected plant with (GAS NPs) with concentration (0.30 mM). Treatment with (SA NPs) caused upregulation significance only of callose gene at (2.5 mM) concentration. The molecular modeling results of used compounds (glycyrrhizic acid ammonium salt and salicylic acid) showed highest score of binding and the best rms define value with a very good binding mode and perfect interactions with amino acids of the three subunits (A, B and C) forming the protein coat of leaf roll virus. Glycyrrhizic acid ammonium salt and salicylic acid nanoparticles could be perfect solution to produce potato plant free virus in-vitro. Further larger studies are needed to investigate the role of the studied compounds in vivo.
Development of a Freshness Index for Fruit Quality Assessment—Using Bell Pepper as a Case Study
Authors: Althaus Bernhard , Blanke Michael
Published: October 2021

(1) Background: This special issue contains new developments in the field of fresh produce quality. Freshness provides the appeal for the consumer to purchase a particular horticultural produce. Freshness is a combination of size, colour, shape, flesh firmness, turgescence and glossiness without wilting, which imply that the produce has been picked only a short while ago and has not suffered any decay during harvest, transport or storage. (2) Objective: The objective of the present work was to develop potential freshness indices. The indices are based on non-invasive, real-time measurements, using changes in surface appearance after harvest, using Bell pepper as the most difficult model. (3) Methods: The selection criteria were a dramatic change in values over 14 days of storage at 17 °C, and either a consistent decrease or increase without peaks and troughs. Only two (out of five) non-invasive techniques were selected, i.e., surface glossiness, measured non-destructively using a luster sensor (type CZ-H72 from Keyence Co., Osaka, Japan) and light reflection spectra, recorded by a spectrometer, whereas the three techniques fruit firmness (penetrometer), colorimeter (e.g., Minolta) and false colour images (profilometer) were excluded. (4) Results: The first technique based on fruit glossiness provides luster values depending on fruit colour. Green Bell pepper fruit with a Fresh Index in excess of 463–490 a.i. can be classified as fresh, and similarly of 525–565 a.i. for yellow and 486–502 a.i. for red pepper fruit. This first Freshness index dropped, indicating a lesser freshness, to less than 100 a.i. after 14 days storage, irrespective of fruit colour, thereby providing a suitable wide, ca. 5-fold, range of parameterization. The second index is based on the difference between the light reflectance peaks at 630–633 nm and at 500 nm, also measured perpendicular to the convex fruit side. These percentage values decreased from >40% with fresh red and yellow Bell pepper to ca. 20% after 14 days storage; similarly, these percentages decreased from ca. 16% to ca. 8% in green pepper fruit, in both cases by a twofold factor. Overall, a third Freshness index could be a combination of luster values larger than 470 a.u. and >40% difference of the light reflectance between 630 nm and 500 nm. (5) Conclusions: Two viz three freshness indices are proposed and thresholds elaborated for Bell pepper fruit. The analysis showed that both indices viz technologies, luster and light reflection spectra, require colour differentiation, i.e., a specific index for yellow, red and green Bell pepper fruit.
Validation of Three Daily Satellite Rainfall Products in a Humid Tropic Watershed, Brantas, Indonesia: Implications to Land Characteristics and Hydrological Modelling
Authors: Wiwoho Bagus Setiabudi , Astuti Ike Sari , Alfarizi Imam Abdul Gani , Sucahyo Hetty Rahmawati
Published: October 2021

A total of three different satellite products, CHIRPS, GPM, and PERSIANN, with different spatial resolutions, were examined for their ability to estimate rainfall data at a pixel level, using 30-year-long observations from six locations. Quantitative and qualitative accuracy indicators, as well as R2 and NSE from hydrological estimates, were used as the performance measures. The results show that all of the satellite estimates are unsatisfactory, giving the NRMSE ranging from 6 to 30% at a daily level, with CC only 0.21–0.36. Limited number of gauges, coarse spatial data resolution, and physical terrain complexity were found to be linked with low accuracy. Accuracy was slightly better in dry seasons or low rain rate classes. The errors increased exponentially with the increase in rain rates. CHIPRS and PERSIANN tend to slightly underestimate at lower rain rates, but do show a consistently better performance, with an NRMSE of 6–12%. CHRIPS and PERSIANN also exhibit better estimates of monthly flow data and water balance components, namely runoff, groundwater, and water yield. GPM has a better ability for rainfall event detections, especially during high rainfall events or extremes (>40 mm/day). The errors of the satellite products are generally linked to slope, wind, elevation, and evapotranspiration. Hydrologic simulations using SWAT modelling and the three satellite rainfall products show that CHIRPS slightly has the daily best performance, with R2 of 0.59 and 0.62, and NSE = 0.54, and the monthly aggregated improved at a monthly level. The water balance components generated at an annual level, using three satellite products, show that CHIRPS outperformed with a ration closer to one, though with a tendency to overestimate up to 3–4× times the data generated from the rainfall gauges. The findings of this study are beneficial in supporting efforts for improving satellite rainfall products and water resource implications.
The Seasonal Water Balance of Western-Juniper-Dominated and Big-Sagebrush-Dominated Watersheds
Authors: Durfee Nicole , Ochoa Carlos G.
Published: October 2021

The combined impacts of woody plant encroachment and climate variability have the potential to alter the water balance in many sagebrush steppe ecosystems in the Western USA, leading to reduced water availability in these already water-scarce regions. This study compared the water-balance characteristics of two adjacent semiarid watersheds in central Oregon, USA: one dominated by big sagebrush and one dominated by western juniper. Precipitation, springflow, streamflow, shallow groundwater levels, and soil moisture were measured. The potential evapotranspiration was calculated using the Hargreaves–Samani method. Potential evapotranspiration and a water-balance approach were used to calculate seasonal actual evapotranspiration. The shallow aquifer recharge was calculated using the Water-Table-Fluctuation-Method. Evapotranspiration, followed by deep percolation, accounted for the largest portion (83% to 86% of annual precipitation) of water output for both watersheds. Springflow and streamflow rates were generally greater at the sagebrush-dominated watershed. Snow-dominated years showed greater amounts of groundwater recharge and deep percolation than years where a larger portion of precipitation fell as rain, even when total annual precipitation amounts were similar. This study’s results highlight the role of vegetation dynamics, such as juniper encroachment, and seasonal precipitation characteristics, on water availability in semiarid rangeland ecosystems.
Cabbage and Sauerkraut Consumption in Adolescence and Adulthood and Breast Cancer Risk among US-Resident Polish Migrant Women
Authors: Pathak Dorothy Rybaczyk , Stein Aryeh D. , He Jian-Ping , Noel Mary M. , Hembroff Larry , Nelson Dorothy A. , Vigneau Fawn , Shen Tiefu , Scott Laura J. , Charzewska Jadwiga , Wajszczyk Bożena , Clark Karen , Rybaczyk Leszek A. , Pathak Bogdan A. , Błaszczyk Dorota , Bankowski Ann , Willett Walter C.
Published: October 2021

Background: Breast cancer (BC) incidence and mortality are lower in Poland than in the United States (US). However, Polish-born migrant women to US approach the higher BC mortality rates of US women. We evaluated the association between consumption of cabbage/sauerkraut foods and BC risk in Polish-born migrants to US. Methods: We conducted a case–control study of BC among Polish-born migrants in Cook County and the Detroit Metropolitan Area. Cases (n = 131) were 20–79 years old with histological/cytological confirmation of invasive BC. Population-based controls (n = 284) were frequency matched to cases on age and residence. Food frequency questionnaires assessed diet during adulthood and age 12–13 years. Odds ratios (OR) and 95% confidence intervals (95% CI) were estimated with conditional logistic regression. Consumption of total, raw/short-cooked, and long-cooked cabbage/sauerkraut foods was categorized as low, medium, or high (frequency of servings/week). Results: Higher consumption of total and raw/short-cooked cabbage/sauerkraut foods, during both adolescence and adulthood, was associated with a significantly lower BC risk. Consumption of long-cooked cabbage/sauerkraut foods was low and not significantly associated with risk. The multivariate OR for total cabbage/sauerkraut consumption, high vs. low (>4 vs. ≤2 servings/week) during adolescence was 0.36 (95% CI = 0.18–0.71, ptrend < 0.01) and 0.50 (95% CI = 0.23–1.06, ptrend = 0.08) during adulthood. For raw/short-cooked cabbage/sauerkraut (>3 vs. ≤1.5 servings/week), the ORs were 0.35 (95% CI = 0.16–0.72, ptrend < 0.01) during adolescence and 0.37 (95% CI = 0.17–0.78, ptrend < 0.01) during adulthood. For joint adolescent/adult consumption of raw/short-cooked cabbage/sauerkraut foods, (high, high) vs. (low, low), the OR was 0.23 (95% CI = 0.07–0.65). The significant association for high adolescent consumption of raw/short-cooked cabbage/sauerkraut foods and reduced BC risk was consistent across all levels of consumption in adulthood. Conclusion: Greater consumption of total and raw/short-cooked cabbage/sauerkraut foods either during adolescence or adulthood was associated with significantly reduced BC risk among Polish migrant women. These findings contribute to the growing literature suggesting a protective effect of a potentially modifiable factor, cruciferous vegetable intake, on breast cancer risk.
Influence of Publicity and Education and Environmental Values on the Green Consumption Behavior of Urban Residents in Tibet
Authors: Ma Huifang , Chen Weidong , Ma Hailin , Yang Hude
Published: October 2021

Faced with ecological environmental issues and a surge in the consumption of products in the Qinghai–Tibetan Plateau, it is necessary to explore the effective driving mechanism of green consumption behavior. This study investigated the impact of publicity and education on green consumption behavior and explored the mediating effects of environmental values and the moderating effects of price sensitivity. A cross-sectional survey was conducted with a sample of 500 questionnaires, which were randomly distributed by stratified random sampling to municipal government departments, public institutions, communities, streets, shops, and supermarket entrances in Lhasa, Shan Nan, and Xigaze in Tibet. Further, structural equation modeling was applied to derive data for statistical analyses. Publicity, education, and environmental values had a significant influence on green consumption behavior. Environmental values play a mediating role in the influence of publicity and education on green consumption behavior. Price sensitivity negatively moderates the relationship between publicity and education and environmental values; when considering the price of green consumption, the positive impact on environmental values is weakened by publicity and education. Furthermore, it moderates the mediating effect of environmental values. Publicity and education remain the primary intervention for promoting green consumption. Especially in areas with ethnic minorities, publicity and education in combination with the characteristics of ethnic areas should be used to promote the traditional Tibetan culture of respect for all life and to live in harmony with nature. Moreover, policies, regulations, and tax subsidies related to green consumption should be improved while reducing the negative impact of prices and other economic factors on the propensity to consume. These findings provide empirical evidence for the complex relationship between government intervention measures and urban residents’ green consumption behavior.
Comparing HIV Post-Exposure Prophylaxis, Testing, and New Diagnoses in Two Australian Cities with Different Lockdown Measures during the COVID-19 Pandemic
Authors: Chow Eric P. F. , Ong Jason J. , Donovan Basil , Foster Rosalind , Phillips Tiffany R. , McNulty Anna , Fairley Christopher K.
Published: October 2021

Australia introduced a national lockdown on 22 March 2020 in response to the COVID-19 pandemic. Melbourne, but not Sydney, had a second COVID-19 lockdown between July and October 2020. We compared the number of HIV post-exposure prophylaxis (PEP) prescriptions, HIV tests, and new HIV diagnoses during these lockdown periods. The three outcomes in 2020 were compared to 2019 using incidence rate ratio. There was a 37% and 46% reduction in PEP prescriptions in Melbourne and Sydney, respectively, with a larger reduction during lockdown (68% and 57% reductions in Melbourne’s first and second lockdown, 60% reduction in Sydney’s lockdown). There was a 41% and 32% reduction in HIV tests in Melbourne and Sydney, respectively, with a larger reduction during lockdown (57% and 61% reductions in Melbourne’s first and second lockdowns, 58% reduction in Sydney’s lockdown). There was a 44% and 47% reduction in new HIV diagnoses in Melbourne and Sydney, respectively, but no significant reductions during lockdown. The reduction in PEP prescriptions, HIV tests, and new HIV diagnoses during the lockdown periods could be due to the reduction in the number of sexual partners during that period. It could also result in more HIV transmission due to substantial reductions in HIV prevention measures during COVID-19 lockdowns.
Longitudinal Effects of Parental Academic Support on Academic Achievement in Korea: Will You Be a Companion or a Manager in Your Children’s Academic Support?
Authors: Jang Yujin , Suh Youngmeen
Published: October 2021

This study addresses the novel concept of two types of parental academic support (PAS), namely, as a companion and as a manager, and identifies the effect of children’s perceived PAS on their psychological attributes and academic achievements. The data include a nationally representative sample of 6836 students from the Korean Education Longitudinal Study 2013. A correlation analysis showed that the two types of PAS had a positive relation with adolescents’ development; however, a structural equation model showed a negative effect of PAS as a manager. Children’s perceived PAS as managers had no effect on their self-regulation or academic achievement after 3 years, surprisingly, was found to increase their amotivation. Children’s perceived PAS as companions had a long-term, positive effect on adolescents’ development and academic achievements. The results suggest that parents should recognize their children’s autonomy and provide academic support on an equal level. Further multidimensional PAS studies should be conducted with more detailed questions.
Role of Essential Oil-Based Mouthwashes in Controlling Gingivitis in Patients Undergoing Fixed Orthodontic Treatment. A Review of Clinical Trials
Authors: Panagiotou Aristeidis , Rossouw P. Emile , Michelogiannakis Dimitrios , Javed Fawad
Published: October 2021

Essential oil (EO)-based mouthwashes have been used for oral health maintenance due to their antimicrobial and anti-inflammatory properties. The aim was to review clinical trials that assessed the role of EO-based mouthwashes in controlling gingivitis in patients undergoing fixed orthodontic treatment (OT). The Patients, Interventions, Control and Outcome (PICO) format was based on the following: (a) P: Patients undergoing fixed OT (b) Intervention: EO-based mouth-wash; Control: Mouthwashes that did not contain EOs or no mouthwash (d) Outcome: Control of gingivitis measured by clinical indices. Databases were searched manually and electronically up to and including May 2021 using different medical subject subheadings. Data screening and extraction were performed. The risk of bias within randomized controlled trials was assessed using the revised Cochrane Collaboration’s risk of bias tool (RoB 2). The Risk of Bias In Non-randomized Studies—of Interventions (ROBINS-I) tool was used for non-randomized controlled trials. Disagreements related to literature search and RoB evaluations were resolved via discussion. Six clinical studies were included. Four studies showed that Listerine® is effective in controlling gingivitis in patients undergoing fixed OT. One study reported that the use of 5% Fructus mume mouthwash resulted in a significant reduction in gingival bleeding. Two mouthwashes that contained 1% Matricaria chamomilla L. and 0.5% Zingiber officinale were also found to be efficient in controlling gingival bleeding. Four, one and one studies had a low, moderate and high RoB, respectively. In conclusion, EO-based mouthwashes seem to be effective for the management of gingivitis among patients undergoing fixed OT. Further well-designed and power-adjusted clinical trials are needed.
Cumulative Exposure to Neighborhood Conditions and Substance Use Initiation among Low-Income Latinx and African American Adolescents
Authors: Lee Eunice , Santiago Anna Maria
Published: October 2021

Purpose: While previous research underscores the important role that neighborhood contexts play for child and adolescent health and well-being, how these neighborhood contexts influence substance use initiation among adolescents from low-income and ethnic minority families has been understudied. Methods: This study is a secondary analysis of data from the Denver Child Study a retrospective survey that uses a natural experiment aimed at assessing neighborhood effects on developmental outcomes of Latinx and African American adolescents (N = 736). Cox cause-specific hazards models were estimated to test: (1) the effects of cumulative exposure to neighborhood social disorder, neighborhood violent and property crime rates, and neighborhood social capital during preadolescence (ages 8–11) on the likelihood of initiating alcohol, cigarette, and marijuana use during adolescence (ages 12–18), after controlling for youth, caregiver, and household factors; and (2) whether the effects of these cumulative neighborhood factors vary by Latinx and African American ethnicity. Results: 5.6% of adolescents in this study initiated cigarette use, 5.4% initiated alcohol use and 5.2% used marijuana for the first time during adolescence. The results indicate that exposure to neighborhood social disorder during preadolescence is a significant risk factor, especially for the initiation of cigarette use (HR = 1.36, 95% CI = 1.062–1.745, p = 0.015) particularly among Latinx adolescents (HR = 1.42, 95% CI = 1.031–1.966, p = 0.032). Conclusions: The findings suggest the need for further research on the relationship between exposure to neighborhood social disorder and adolescent substance use initiation in order to develop and implement community-based prevention and intervention programs to reduce substance use initiation and facilitate healthy adolescent development.
Relationships, Decisions, and Physical Effort in the Marro Traditional Sporting Game: A Multimodal Approach
Authors: Martín-Martínez David , Lavega-Burgués Pere , Salas-Santandreu Cristòfol , Duran-Delgado Conxita , Prat Queralt , Damian-Silva Sabrine , Machado Leonardo , Aires-Araujo Pablo , Muñoz-Arroyave Verónica , Lapuente-Sagarra Manuel , Serna Jorge , Pic Miguel
Published: October 2021

The purpose of this study was to examine the players’ decisions-making in Marro (a Traditional Sporting Game) through a multimodal approach. Each player’s decision-making assumes specific accelerations and decelerations associated with different effort. The research objectives were: (i) to study the decision-making associated with the roles of Hunter and Hare; (ii) to know the physical effort by the roles (Hunters and Hares); (iii) to reveal T-Patterns in the multimodal strategic approach (integrated with decisions and different physical effort) with a direct incidence on the scoring by roles. The study was performed with 22 male and 2 female players aged 18 to 25 (M = 19.4; SD = 1.3). The Marro game was played by two groups for eight minutes. An observational methodology was used, through a type III design. The observational design was nomothetic, one-time, and multidimensional. An ‘ad hoc’ tool was built to ensure the data quality. Univariate analyses were performed using Crosstabs Command, with adjusted residuals (AR), Classification Trees (Chaid model) and T-Pattern Analysis (TPA). Significant differences were found between matches using the scoring (p < 0.001; ES = 0.26), role (p < 0.001; ES = 0.31), or the organic variables of the study, the speed (p < 0.001; ES = 0.73), the metabolic power and the acceleration/deceleration the speed (p = 0.023; ES = 0.43), while the predictive model pointed to the variable role (p < 0.001) as the main factor responsible for the model growth. TPA (p < 0.005) revealed differences attributable to internal logic in the yellow (first match) and orange (second match) teams, while organic variables were more changeable in the violet (first match) and green (second match) teams. This study advances the individualization of the decision-making process. These results may be useful to better understand the internal of functioning of the Marro game 360° since the use of various methodologies and variables (multimodal approach) provided original findings.
A Multi-Pronged Evaluation of a Healthy Food Access Initiative in Central Texas: Study Design, Methods, and Baseline Findings of the FRESH-Austin Evaluation Study
Authors: Janda Kathryn M. , Ranjit Nalini , Salvo Deborah , Nielsen Aida , Akhavan Nika , Diaz Martha , Lemoine Pablo , Casnovsky Joy , van den Berg Alexandra
Published: October 2021

Food insecurity and limited healthy food access are complex public health issues and warrant multi-level evaluations. The purpose of this paper was to present the overall study design and baseline results of the multi-pronged evaluation of a healthy food access (i.e., Fresh for Less (FFL)) initiative in Central Texas. The 2018–2021 FRESH-Austin study was a natural experiment that utilized a cluster random sampling strategy to recruit three groups of participants (total n = 400): (1) customers at FFL assets, (2) residents that lived within 1.5 miles of an FFL asset, and (3) residents from a comparison community. Evaluation measures included annual cohort surveys, accelerometers and GPS devices, store-level audits, and built environment assessments. Data are being used to inform and validate an agent-based model (ABM) to predict food shopping and consumption behaviors. Sociodemographic factors and food shopping and consumption behaviors were similar across the three groups; however, customers recruited at FFL assets were lower income and had a higher prevalence of food insecurity. The baseline findings demonstrate the need for multi-level food access interventions, such as FFL, in low-income communities. In the future, ABM can be used as a cost-effective way to determine potential impacts of future large-scale food environment programs and policies.
Technology Acceptance and Leadership 4.0: A Quali-Quantitative Study
Authors: Molino Monica , Cortese Claudio G. , Ghislieri Chiara
Published: October 2021

With the rapid advancement of Industry 4.0, new technologies are changing the nature of work and organizations. Nevertheless, technology acceptance is still an open issue and research, and practice interventions should investigate its antecedents and implement actions in order to reduce the risks of resistance and foster acceptance and effective usage of the new tools and systems. This quali-quantitative study was aimed at exploring perceptions about Industry 4.0 and its transformations and investigating job antecedents of technology acceptance. Whilst not many studies in the literature on technology acceptance have considered workers’ well-being, in this study, its association with work engagement has also been examined. The qualitative study used focus groups to collect perceptions of 14 key roles in a company that was implementing Industry 4.0. In the same company, the quantitative study involved 263 employees who filled in a questionnaire. The results confirmed that both job resources, namely supervisor support and role clarity, were antecedents of technology acceptance, which, in turn, was associated with work engagement. This study provides useful suggestions for interventions aimed at foster technology acceptance and workers’ well-being in companies that are facing Industry 4.0 transformations. Particularly, investments in both leadership 4.0 development and communication programs are essential.
High Influencing Pattern Discovery over Time Series Data
Authors: Fang Dianwu , Wang Lizhen , Wang Jialong , Wang Meijiao
Published: October 2021

A spatial co-location pattern denotes a subset of spatial features whose instances frequently appear nearby. High influence co-location pattern mining is used to find co-location patterns with high influence in specific aspects. Studies of such pattern mining usually rely on spatial distance for measuring nearness between instances, a method that cannot be applied to an influence propagation process concluded from epidemic dispersal scenarios. To discover meaningful patterns by using fruitful results in this field, we extend existing approaches and propose a mining framework. We first defined a new concept of proximity to depict semantic nearness between instances of distinct features, thus applying a star-shaped materialized model to mine influencing patterns. Then, we designed attribute descriptors to perceive attributes of instances and edges from time series data, and we calculated the attribute weights via an analytic hierarchy process, thereby computing the influence between instances and the influence of features in influencing patterns. Next, we constructed influencing metrics and set a threshold to discover high influencing patterns. Since the metrics do not satisfy the downward closure property, we propose two improved algorithms to boost efficiency. Extensive experiments conducted on real and synthetic datasets verified the effectiveness, efficiency, and scalability of our method.
A Spatio-Temporal Schedule-Based Neural Network for Urban Taxi Waiting Time Prediction
Authors: You Lan , Guan Zhengyi , Li Na , Zhang Jiahe , Cui Haibo , Claramunt Christophe , Cao Rui
Published: October 2021

Taxi waiting times is an important criterion for taxi passengers to choose appropriate pick-up locations in urban environments. How to predict the taxi waiting time accurately at a certain time and location is the key solution for the imbalance between the taxis’ supplies and demands. Considering the life schedule of urban residents and the different functions of geogrid regions, the research developed in this paper introduces a spatio-temporal schedule-based neural network for urban taxi waiting time prediction. The approach integrates a series of multi-source data from taxi trajectories to city points of interest, different time frames and human behaviors in the city. We apply a grid-based and functional structuration of an urban space that provides a lower-level data representation. Overall, the neural network model can dynamically predict the waiting time of taxi passengers in real time under some given spatio-temporal constraints. The experimental results show that the granular-based grids and spatio-temporal neural network can effectively predict and optimize the accuracy of taxi waiting times. This work provides a decision support for intelligent travel predictions of taxi waiting time in a smart city.
Local Climate Zones, Land Surface Temperature and Air Temperature Interactions: Case Study of Hradec Králové, the Czech Republic
Authors: Středová Hana , Chuchma Filip , Rožnovský Jaroslav , Středa Tomáš
Published: October 2021

The current application of local climate zones (LCZs) often ends with (inter)zonal comparation of land surface temperature (LST) or air temperature (AT). LST evaluation employs an enhanced concept of LCZs together with cluster analysis for LCZs grouped based on LST. The paper attempts to combine them into a complex approach derived from the case study on a medium-sized Central European city (Hradec Králové, the Czech Republic). In particular, the paper addresses the following. (i) The relation of LST and AT, when the daily course of temperature profile ranging clear off the surface up to 2 m was fitted by a rational 2D function. The obtained equation enables derivation of the AT from LST and vice versa. (ii) The differences in thermal response of LCZs based on LST or AT, where the highest average LST and average maximum LST show LCZs 10, 2, 3 and 8, i.e., with a significant proportion of artificial surfaces. The cluster of LCZs with a significant representation of vegetation, LCZs 9, B, D, A and G, have significantly lower LST. (iii) The contribution of LCZs to understanding of LST/AT relation and whether their specific relation could be expected in particular LCZs, when subsequent interaction assessment of LST and AT revealed statistically their significant correlation in LCZs for certain cases.
Discovery of a Potent and Selective JNK3 Inhibitor with Neuroprotective Effect Against Amyloid β-Induced Neurotoxicity in Primary Rat Neurons
Authors: Jun Joonhong , Baek Jihyun , Yang Songyi , Moon Hyungwoo , Kim Hyejin , Cho Hyunwook , Hah Jung-Mi
Published: October 2021

As members of the MAPK family, c-Jun-N-terminal kinases (JNKs) regulate the biological processes of apoptosis. In particular, the isoform JNK3 is expressed explicitly in the brain at high levels and is involved in the pathogenesis of neurodegenerative diseases such as Alzheimer’s disease (AD) and Parkinson’s disease (PD). In this study, we prepared a series of five 6-dihydroxy-1H-benzo[d]imidazoles as JNK3 inhibitors and found them have potential as neuroprotective agents. Following a previous lead scaffold, benzimidazole moiety was modified with various aryl groups and hydroxylation, and the resulting compounds exhibited JNK3 inhibitory activity with improved potency and selectivity. Out of 37 analogues synthesized, (S)-cyclopropyl(3-((4-(2-(2,3-dihydrobenzo[b][1,4]dioxin -6-yl)-5,6-dihydroxy-1H-benzo[d]imidazol-1-yl)pyrimidin-2-yl)amino) piperidin-1-yl)methanone (35b) demonstrated the highest JNK3 inhibition (IC50 = 9.7 nM), as well as neuroprotective effects against Aβ-induced neuronal cell death. As a protein kinase inhibitor, it also showed excellent selectivity over other protein kinases including isoforms JNK1 (>1000 fold) and JNK2 (−10 fold).
Optimization of Advanced Live-Cell Imaging through Red/Near-Infrared Dye Labeling and Fluorescence Lifetime-Based Strategies
Authors: Bénard Magalie , Schapman Damien , Chamot Christophe , Dubois Fatéméh , Levallet Guénaëlle , Komuro Hitoshi , Galas Ludovic
Published: October 2021

Fluorescence microscopy is essential for a detailed understanding of cellular processes; however, live-cell preservation during imaging is a matter of debate. In this study, we proposed a guide to optimize advanced light microscopy approaches by reducing light exposure through fluorescence lifetime (τ) exploitation of red/near-infrared dyes. Firstly, we characterized key instrumental elements which revealed that red/near-infrared laser lines with an 86x (Numerical Aperture (NA) = 1.2, water immersion) objective allowed high transmission of fluorescence signals, low irradiance and super-resolution. As a combination of two technologies, i.e., vacuum tubes (e.g., photomultiplier) and semiconductor microelectronics (e.g., avalanche photodiode), type S, X and R of hybrid detectors (HyD-S, HyD-X and HyD-R) were particularly adapted for red/near-infrared photon counting and τ separation. Secondly, we tested and compared lifetime-based imaging including coarse τ separation for confocal microscopy, fitting and phasor plot analysis for fluorescence lifetime microscopy (FLIM), and lifetimes weighting for enhanced stimulated emission depletion (STED) nanoscopy, in light of red/near-infrared multiplexing. Mainly, we showed that the choice of appropriate imaging approach may depend on fluorochrome number, together with their spectral/lifetime characteristics and STED compatibility. Photon-counting mode and sensitivity of HyDs together with phasor plot analysis of fluorescence lifetimes enabled the flexible and fast imaging of multi-labeled living H28 cells. Therefore, a combination of red/near-infrared dyes labeling with lifetime-based strategies offers new perspectives for live-cell imaging by enhancing sample preservation through acquisition time and light exposure reduction.
Unraveling Protein Interactions between the Temperate Virus Bam35 and Its Bacillus Host Using an Integrative Yeast Two Hybrid–High Throughput Sequencing Approach
Authors: Lechuga Ana , Lood Cédric , Berjón-Otero Mónica , del Prado Alicia , Wagemans Jeroen , van Noort Vera , Lavigne Rob , Salas Margarita , Redrejo-Rodríguez Modesto
Published: October 2021

Bacillus virus Bam35 is the model Betatectivirus and member of the family Tectiviridae, which is composed of tailless, icosahedral, and membrane-containing bacteriophages. Interest in these viruses has greatly increased in recent years as they are thought to be an evolutionary link between diverse groups of prokaryotic and eukaryotic viruses. Additionally, betatectiviruses infect bacteria of the Bacillus cereus group, which are known for their applications in industry and notorious since it contains many pathogens. Here, we present the first protein–protein interactions (PPIs) network for a tectivirus–host system by studying the Bam35–Bacillus thuringiensis model using a novel approach that integrates the traditional yeast two-hybrid system and high-throughput sequencing (Y2H-HTS). We generated and thoroughly analyzed a genomic library of Bam35′s host B. thuringiensis HER1410 and screened interactions with all the viral proteins using different combinations of bait–prey couples. Initial analysis of the raw data enabled the identification of over 4000 candidate interactions, which were sequentially filtered to produce 182 high-confidence interactions that were defined as part of the core virus–host interactome. Overall, host metabolism proteins and peptidases were particularly enriched within the detected interactions, distinguishing this host–phage system from the other reported host–phage PPIs. Our approach also suggested biological roles for several Bam35 proteins of unknown function, including the membrane structural protein P25, which may be a viral hub with a role in host membrane modification during viral particle morphogenesis. This work resulted in a better understanding of the Bam35–B. thuringiensis interaction at the molecular level and holds great potential for the generalization of the Y2H-HTS approach for other virus–host models.
Resolution of Two Steps in Botulinum Neurotoxin Serotype A1 Light Chain Localization to the Intracellular Plasma Membrane
Authors: Gardner Alexander , Tepp William H. , Bradshaw Marite , Barbieri Joseph T. , Pellett Sabine
Published: October 2021

Botulinum neurotoxin serotype A (BoNT/A) is the most potent protein toxin to humans. BoNT/A light chain (LC/A) cleavage of the membrane-bound SNAP-25 has been well-characterized, but how LC/A traffics to the plasma membrane to target SNAP-25 is unknown. Of the eight BoNT/A subtypes (A1–A8), LC/A3 has a unique short duration of action and low potency that correlate to the intracellular steady state of LC/A, where LC/A1 is associated with the plasma membrane and LC/A3 is present in the cytosol. Steady-state and live imaging of LC/A3-A1 chimeras identified a two-step process where the LC/A N terminus bound intracellular vesicles, which facilitated an internal α-helical-rich domain to mediate LC/A plasma membrane association. The propensity of LC/A variants for membrane association correlated with enhanced BoNT/A potency. Understanding the basis for light chain intracellular localization provides insight to mechanisms underlying BoNT/A potency, which can be extended to applications as a human therapy.
The Digital Dimension of Mobilities: Mapping Spatial Relationships between Corporeal and Digital Displacements in Barcelona
Authors: Brandajs Fiammetta
Published: October 2021

This paper explores the ways in which technologies reshape everyday activities, adopting a mobility perspective of the digital environment, which is reframed in terms of the constitutive/substitutive element of corporeal mobility. We propose the construction of a Digital Mobility Index, quantified by measuring the usage typology in which the technology is employed to enable mobility. Through a digital perspective on mobilities, it is possible to investigate how embodied practices and experiences of different modes of physical or virtual displacements are facilitated and emerge through technologies. The role of technologies in facilitating the anchoring of mobilities, transporting the tangible and intangible flow of goods, and in mediating social relations through space and time is emphasized through analysis of how digital usage can reproduce models typical of the neoliberal city, the effects of which in terms of spatial (in)justice have been widely discussed in the literature. The polarization inherent to the digital divide has been characterized by a separation between what has been called the “space of flows” (well connected, mobile, and offering more opportunities) and the “space of places” (poorly connected, fixed, and isolated). This digital divide indeed takes many forms, including divisions between classes, urban locations, and national spaces. By mapping “hyper- and hypo-mobilized” territories in Barcelona, this paper examines two main dimensions of digital inequality, on the one hand identifying the usage of the technological and digital in terms of the capacity to reach services and places, and on the other, measuring the territorial demographic and economic propensity to access to ICT as a predictive insight into the geographies of the social gap which emerge at municipal level. This approach complements conventional data sources such as municipal statistics and the digital divide enquiry conducted in Barcelona into the underlying digital capacities of the city and the digital skills of the population.
Multimorbidity and Anxiety Symptoms among Adults Aged 50 Years and Over from Six Low- and Middle-Income Countries
Authors: Smith Lee , López Sánchez Guillermo F. , Shin Jae Il , Soysal Pinar , Veronese Nicola , Kostev Karel , Jacob Louis , Oh Hans , Schuch Felipe , Butler Laurie , Barnett Yvonne , Law Christopher Tejun , Koyanagi Ai
Published: October 2021

Currently, there are limited data on the association between multimorbidity (i.e., ≥ 2 chronic conditions) and anxiety, especially among the older population in low- and middle-income countries (LMICs). Thus, the aim of the present study was to examine the association between multimorbidity and anxiety symptoms in a large sample of adults aged ≥ 50 years from six LMICs (China, India, Ghana, Mexico, Russia, South Africa). Cross-sectional, nationally representative, community-based data from the Study on Global Ageing and Adult Health (SAGE) were analysed. A total of 11 chronic physical conditions were assessed. Anxiety symptoms referred to extreme/severe problems with worry or anxiety in the past 30 days. Multivariable logistic regression and meta-analyses were conducted. Data on 34,129 adults aged ≥ 50 years were analysed (mean (SD) age 62.4 (16.0) years; 52.1% females). Compared with no chronic conditions, 2, 3, 4, and ≥ 5 chronic conditions were significantly associated with 1.47 (95% CI = 1.08–1.98), 2.46 (95% CI = 1.74–3.47), 3.04 (95% CI = 2.15–4.30), and 4.70 (95% CI = 2.99–7.38) times higher odds of anxiety symptoms, respectively. A country-wise analysis showed that multimorbidity was significantly associated with anxiety symptoms in all six countries (OR = 1.78–12.39) with the overall estimate based on a meta-analysis being OR = 2.29 (95% CI = 1.71–3.07). Multimorbidity was associated with higher odds of anxiety symptoms among older adults in LMICs. Future longitudinal studies are warranted to assess the temporal associations and mechanisms underlying this association.
Catheter-Directed Therapies in Patients with Pulmonary Embolism: Predictive Factors of In-Hospital Mortality and Long-Term Follow-Up
Authors: Ribas Jesús , Valcárcel Joana , Alba Esther , Ruíz Yolanda , Cuartero Daniel , Iriarte Adriana , Mora-Luján José María , Huguet Marta , Cerdà Pau , Martínez-Yélamos Sergio , Corbella Xavier , Santos Salud , Riera-Mestre Antoni
Published: October 2021

(1) Background: Catheter-directed therapies (CDT) may be considered for selected patients with pulmonary embolism (PE); (2) Methods: Retrospective observational study including all consecutive patients with acute PE undergoing CDT (mechanical or pharmacomechanical) from January 2010 through December 2020. The aim was to evaluate in-hospital and long-term mortality and its predictive factors; (3) Results: We included 63 patients, 43 (68.3%) with high-risk PE. All patients underwent mechanical CDT and, additionally, 27 (43%) underwent catheter-directed thrombolysis. Twelve (19%) patients received failed systemic thrombolysis (ST) prior to CDT, and an inferior vena cava (IVC) filter was inserted in 28 (44.5%) patients. In-hospital PE-related and all-cause mortality rates were 31.7%; 95% CI 20.6–44.7% and 42.9%; 95% CI 30.5–56%, respectively. In multivariate analysis, age > 70 years and previous ST were strongly associated with PE-related and all-cause mortality, while IVC filter insertion during the CDT was associated with lower mortality rates. After a median follow-up of 40 (12–60) months, 11 more patients died (mortality rate of 60.3%; 95% CI 47.2–72.4%). Long-term survival was significantly higher in patients who received an IVC filter; (4) Conclusions: Age > 70 years and failure of previous ST were associated with mortality in acute PE patients treated with CDT. In-hospital and long-term mortality were lower in patients who received IVC filter insertion.
Sinus Lift and Implant Insertion on 3D-Printed Polymeric Maxillary Models: Ex Vivo Training for In Vivo Surgical Procedures
Authors: Nica Diana Florina , Gabor Alin Gabriel , Duma Virgil-Florin , Tudericiu Vlad George , Tudor Anca , Sinescu Cosmin
Published: October 2021

Background and Objectives: The aim of this study is to demonstrate the increased efficiency achieved by dental practitioners when carrying out an ex vivo training process on 3D-printed maxillaries before performing in vivo surgery. Materials and Methods: This developed ex vivo procedure comprises the following phases: (i) scanning the area of interest for surgery; (ii) obtaining a 3D virtual model of this area using Cone Beam Computed Tomography (CBCT); (iii) obtaining a 3D-printed model (based on the virtual one), on which (iv) the dental practitioner simulates/rehearses ex vivo (most of) the surgery protocol; (v) assess with a new CBCT the 3D model after simulation. The technical steps of sinus augmentation and implant insertion could be performed on the corresponding 3D-printed hemi-maxillaries prior to the real in vivo surgery. Two study groups were considered, with forty patients divided as follows: Group 1 comprises twenty patients on which the developed simulation and rehearsal procedure was applied; Group 2 is a control one which comprises twenty patients on which similar surgery was performed without this procedure (considered in order to compare operative times without and with rehearsals). Results: Following the ex vivo training/rehearsal, an optimal surgery protocol was developed for each considered case. The results of the surgery on patients were compared with the results obtained after rehearsals on 3D-printed models. The performed quantitative assessment proved that, using the proposed training procedure, the results of the in vivo surgery are not significantly different (p = 0.089) with regard to the ex vivo simulation for both the mezio-distal position of the implant and the distance from the ridge margin to sinus window. On the contrary, the operative time of Group 1 was reduced significantly (p = 0.001), with an average of 20% with regard to in vivo procedures performed without rehearsals (on the control Group 2). Conclusions: The study demonstrated that the use of 3D-printed models can be beneficial to dental surgeon practitioners, as well as to students who must be trained before performing clinical treatments.
Minor Sphincter Sparing Surgery for Successful Closure of Perianal Fistulas in Patients with Crohn’s Disease
Authors: Merten Jennifer , Eichelmann Ann-Kathrin , Mennigen Rudolf , Flammang Isabelle , Pascher Andreas , Rijcken Emile
Published: October 2021

The purpose of this study is to demonstrate that repetitive minor surgical procedures allow for a high rate of permanent closure of perianal fistulas in patients with Crohn’s disease (CD). Patients with perianal fistulizing CD (PFCD) who underwent perianal surgery at the University Hospital of Muenster between 2003 and 2018 were assessed for fistula characteristics and surgical procedures. We included 45 patients (m:f = 28:17) with a mean age of 27 years at first fistula appearance. Of these, 49% suffered from a complex fistula. An average of 4.2 (1–14) procedures were performed, abscess incisions and fistula seton drainages included. Draining setons were left in place for 5 (1–54) months, until fistula closure. Final surgical techniques were fistulotomy (31.1%), seton removal with sustained biological therapy (26.7%), Anal Fistula Plug (AFP) (17.8%), Over-The Scope-Clip proctology (OTSC) (11.1%), and mucosa advancement flap (4.4%). In 8.9% of cases, the seton was kept as permanent therapy. The time from first to last surgery was 18 (0–182) months and the median follow-up time after the last surgery was 90 (15–200) months. The recurrence rate was 15.5% after 45 (17–111) months. Recurrent fistulas healed after another 1.86 (1–2) surgical re-interventions. The final success rate was 80%. Despite biological treatment, PFCD management remains challenging. However, by repeating minor surgical interventions over a prolonged period of time, high permanent healing rates can be achieved.