bluuebunny/crossref_metadata_2025 huggingface dataset:
Comparative study on lesions of reproductive disorders of cows and female dromedary camels slaughtered at Addis Ababa, Adama and Akaki abbatoirs with bacterial isolation and characterization
Authors: Mandefro Aynalem , Ayana Tilaye Demissie , Hunderra Gemechu Chala , Gebrezihar Tadesse Gidey , Boru Bulto Giro , Desta Natnael Teshager
Published: September 2020

Background: Reproduction is a basic prerequisite to efficient livestock production. Reproductive performance depends upon the normal structure and function of genital organs. Methods: A cross-sectional study was conducted from November 2016 to May 2017 to identify and compare the frequency of pathological lesions in the reproductive tract and to isolate bacteria associated to uterine lesions in female dromedary camels and cows slaughtered at Akaki camel slaughter house and Addis Ababa and Adama municipal abattoirs. Purposive sampling technique was used to collect 280 reproductive tracts from cows and female dromedary camels. Following gross inspection at abattoirs, tissue samples with lesion were collected for histopathological and bacteriological investigation. Result: Various pathological lesions with different degrees of severity were observed in 48 (34.2 %) and 51 (36.4%) of dromedary camels and cows, respectively. Uterine lesions were the most prevalent (21.4%) lesions observed in dromedary camels followed by ovarian lesions (7.14%); while in cows, ovarian lesions were the major prevalent (16.4%) lesions followed by uterine lesion (14.2%). The result showed that there were 56 bacteria isolated from cows uterine lesion with Staphylococcus species (28.5%), Streptococci species (19.6%), Coynebacterium species (8.9%), Escherichia coli (26.78%), Salmonella species (10.7%) and Klebsiella species (5.35%) being the prominent isolates; while in camels, there were 45 bacteria isolated with Escherichia coli (35.5%), Staphylococcus species (26.6%), Streptococcus species (13.3%), Pseudomonas species (6.6 %), Proteus species (4.4%), Salmonella species (8.8%) and Klebsiella species (4.4%) being the most frequently isolated. The result showed that the major isolates were similar with slightly higher in cows. Histopathologically, endometrial glands degeneration, sloughing of epithelium, peri-glandular cuffing and infiltrations of inflammatory cell were some of characteristic changes observed in uterus. Conclusions: Pathological lesions in reproductive organs in female dromedary camels and cows showed great prevalence, with similarity in bacterial isolates between the two species. The role of each reproductive lesions and bacterial isolates incriminated as causes of reproductive failures in this livestock species needs further investigation.
Semi-embedded Valve Anastomosis A new anti-reflux anastomotic method after proximal gastrectomy for adenocarcinoma of the oesophagogastric junction
Authors: Wang Baohua , Wu Yupeng , Wang Haijun , Zhang Haiqiang , Wang Liting , Zhang Zhanxue
Published: September 2020

Background

There is a high probability of gastroesophageal reflux after laparoscopic proximal gastrectomy for Siewert Ⅱ AEG. Various anti-reflux anastomotic methods are emerging in clinical practice; however, none of them have been widely accepted. We have innovated a new type of anti-reflux anastomotic method, named semi-embedded valve anastomosis. The aim of this study was to explore the feasibility and anti-reflux effect of the new anastomotic method.

Methods

The clinical data of 28 patients with Siewert II AEG who were treated by semi-embedded valve anastomosis were collected. The key point of the operation is to reconstruct a simulated valve and form an anti-reflux barrier similar to the physiological mechanism. The GerdQ gastroesophageal reflux questionnaire and classification of gastroesophageal reflux under electron microscopy were used to evaluate gastroesophageal reflux after the operation.

Results

The mean operative duration was 164.3±19.0 min, the median intraoperative haemorrhage volume was 65 ml, the average number of lymph nodes dissected was 23±2.6, the time for valve construction was 15.8±3.2 min, the time for anastomotic reconstruction was 35.4±4.8 min, the median time to first flatus was 3 d, and the median hospitalization duration was 12 d. There was one case of postoperative anastomotic stenosis. The GerdQ score [median (range)] was as follows: 2 (0-6), preoperation; 0 (0-8), 1 month postoperation; 2 (0-12), 3 months postoperation; and 3 (0-12), 6 months postoperation. The Wilcoxon signed-rank sum test was carried out at different times after the operation and the day before the operation, and the differences were not significant (P<0.05). There was one case of grade B gastroesophageal reflux according to the LA classification system among the gastrofibroscopic re-examination reports of 28 cases.

Conclusion

Semi-embedded valve anastomosis is safe and feasible after proximal gastrectomy for Siewert II AEG and has good anti-reflux effects.
Little Akaki River Sediment Enrichment with Heavy Metals, Pollution Load and Potential Ecological Risks in Downstream, Central Ethiopia
Authors: Mekuria Deshu Mamo , Kassegne Alemnew Berhanu Berhanu , Asfaw Seyoum Leta Leta
Published: September 2020

Background: The Addis Ababa City’s river ecosystem is under extreme pressure as a result of inappropriate practices of dumping domestic and industrial wastes; thus, threatening its ability to maintain basic ecological, social and economic functions. Little Akaki River which drains through Addis Ababa City receives inorganic and organic pollutants from various anthropogenic sources. Most of inorganic pollutants such as toxic heavy metals relased into the river are eventually adsorbed and settled in the sediment. The objective of this study was to evaluate the enrichment levels, pollution load and ecological risks of selected heavy metals ( Zn, Cr, Cd and Pb) using various indices .Results: The mean concentrations of heavy metals in Little Akaki River sediment were: Zn (78.96±0.021 - 235.2 ±0.001mg / kg); Cr (2.19±0.014 - 440.8±0.003 mg / kg); Cd (2.09±0.001-4.16 ±0.0001mg / kg) and Pb (30.92±0.018 -596.4±0.066 mg / kg). Enrichment factor values indicated that sediments were moderate to significant enrichment with Zn and Cr; moderate to very high enrichment with Pb, and very high enrichment in all sampled sites with Cd. Geo-accumulation index and contamination factor values indicated that the sediments were moderate to very high contamination with toxic Cd and Pb. The decreasing order of pollution load index (PLI) in downstream was: (S9) > (S4) > (S8) > (S3)> (S6) > (S10) > (S5) > (S2)> (S7) > (S1). PLI and hierarchal cluster analysis revealed that highest pollution load occurred in the lower course of the river (S9) which may be due to metals inputs from anthropogenic sources; hence, its quality was deteriorated showing that the site is polluted. The ecological risk (RI =350.62) suggested that the contaminated Little Akaki River (LAR) sediment can pose considerable ecological risks of pollution.Conclusions: The concentrations of Zn, Cr, Cd and Pb in Little Akaki River sediment surpassed eco-toxicological guideline limits of USEPA (threshold effect concentration) and CCME (Interim Sediment Quality Guidelines). Thus, the contaminated sediments can occasionally pose adverse biological effects on sediment dwelling organisms. Thus, measures must be taken to regulate discharge of untreated wastes into river and surrounding environment.
Transcriptome response comparison between vector and non-vector aphids after feeding on virus-infected wheat plants
Authors: Li Dandan , Zhang Chi , Tong Zeqian , Su Dan , Zhang Gaisheng , Zhang Shize , Zhao Huiyan , Hu Zuqing
Published: September 2020

Background

Plant viruses maintain intricate interactions with their vector and non-vector insects and can impact the fitness of insects. However, the details of their molecular and cellular mechanisms have not been studied well. We compared the transcriptome-level responses in vector and non-vector aphids ( Schizaphis graminum and Rhopalosiphum padi ) after feeding on wheat plants with viral infections ( Barley Yellow Dwarf Virus (BYDV)/ Wheat dwarf virus (WDV)). We conducted differentially expressed gene (DEG) annotation analyses and observed DEGs related to immune pathway, growth, development, and reproduction. And we conducted cloning and bioinformatic analyses of the key DEG involved in immune.

Results

For all differentially expressed gene analyses, the numbers of DEGs related to immune, growth, development, reproduction and cuticle were higher in vector aphids than in non-vector aphids. STAT5B (signal transducer and activator of transcription 5B), which is involved in the JAK-STAT pathway, was upregulated in R. padi exposed to WDV. The cloning and bioinformatic results indicated that the RpSTAT5B sequence contains a 2082 bp ORF encoding 693 amino acids. The protein molecular weight is 79.1 kD and pI is 8.13. Analysis indicated that RpSTAT5B is a non-transmembrane protein and a non-secreted protein. Homology and evolutionary analysis indicated that RpSTAT5B was closely related to Rhopalosiphum maidis.

Conclusions

Unigene expression analysis showed that the total number of differentially expressed genes (DEGs) in the vector aphids was larger than that in the non-vector aphids. Functional enrichment analysis showed that the DEGs related to immunity, growth and reproduction in vector aphids were larger than those in non-vector aphids, and the differentially expressed genes related to immune were up-regulated. This study provides a basis for the evaluation of the response mechanisms of vector/non-vector insects to plant viruses.
Life Satisfaction among a Clinical Eating Disorder Population
Authors: Claydon Elizabeth , DeFazio Caterina , Lilly Christa L. , Zullig Keith J.
Published: September 2020

Background: The primary objective was to understand life satisfaction (LS) of patients with eating disorders (EDs) in relation to eating pathology severity, personal/familial ED history, and key demographic and anthropometric variables. Methods: Participants (N=60) completed the Satisfaction with Life Scale (SWLS), the Eating Pathology Severity Index (EPSI), and demographic questionnaires. Bivariate associations via correlations and multiple linear regression models were used to explore these relationships. Results: The SWLS mean score was 3.7 out of 7, suggesting it is below the population-based norm. LS was positively statistically significantly associated with private insurance, past ED, EPSI muscle building, EPSI restricted eating, and EPSI negative attitudes. When included in multiple linear regression, the model explained 33% of the variability of LS [F (7, 56) = 3.4, p = 0.0054, R2 = 0.33]. EPSI muscle building remained the strongest predictor (β = 0.13, p = 0.04). Conclusions: Based on the data, individuals who have/have had EDs scored lower on the SWLS than the general population. Individuals scoring within this range typically experience significant issues in several areas of life or a substantial issue in one area.
Risk factors affecting the 1-year outcomes of minor ischemic stroke: results from Xi’an stroke registry study of China[1]
Authors: Liu Zhongzhong , Lin Wenjuan , Lu Qingli , Wang Jing , Liu Pei , Lin Xuemei , Wang Fang , Shi Yaling , Wang Qing , Liu Guozheng , Wu Songdi
Published: September 2020

Background: The incidences of stroke recurrence, disability, and all-cause death of patients with minor ischemic stroke (MIS) remain problematic. The aim of the present was to identify risk factors associated with adverse outcomes at 1-year after MIS in the Xi’an region of China. Methods: The cohort of this prospective cohort study included MIS patients aged 18–97 years with a National Institutes of Health Stroke Scale (NIHSS) score of ≤ 3 who were treated in any of four hospitals in Xi’an region of China between January and December 2015. The 1-year percentage of stroke recurrence, disability, and all-cause death were evaluated. Multivariate logistic regression analysis was performed to assess the association between the identified risk factors and clinical outcomes. Results: Among the 1,121 patients included for analysis, the percentage of stroke recurrence, disability, and all-cause death at 1 year after MIS were 3.4% (38/1121), 9.3% (104/1121), and 3.3% (37/1121), respectively. Multivariate logistic regression analysis identified age, current smoking, and pneumonia as independent risk factors for stroke recurrence. Age, pneumonia, and alkaline phosphatase were independent risk factors for all-cause death. Independent risk factors for disability were age, pneumonia, NIHSS score on admission, and leukocyte count. Conclusions: The 1-year outcomes of MIS is not optimistic in the Xi’an region of China, especially high percentage of disability. In this study, we found the risk factors affecting 1-year stroke recurrence, disability and, all-cause death which need further verification in the subsequent studies.
Application of Ex Vivo Liver Resection Followed by the Autotransplantation Technique in Radical Resection of Gastric Cancer Liver Metastases: A Case Report
Authors: Wang Hong , Zhang Chengcheng , Ou Yanjiao , Zhang Leida
Published: September 2020

Background Radical resection of gastric cancer liver metastases can increase the 5-year survival rate of GCLM patients. However, patients may lose the theoretical feasibility of surgery due to the critical location of liver metastasis in some cases. Case presentation A 29-year-old female had a chief complaint of chronic abdominal pain for one year. Abdominal CT and MRI examinations suggested a mass of unknown pathological nature located between the first and second hila and the margin of the lower segment of the right lobe of the liver. The anterior wall of the gastric antrum was unevenly thickened. The diagnosis of (gastric antrum) intramucosal well-differentiated adenocarcinoma was histopathologically confirmed by puncture biopsy with gastroscopy guidance. The pathological properties of the liver mass remained unclear before surgery. She underwent radical resection (excision of both gastric tumors and ELRA simultaneously）followed by XELOX adjuvant chemotherapy. Without serious postoperative complications, the patient was successfully discharged on the 20th day after the operation. Pathological examination of the excised specimen indicated that gastrectomy with D2 lymph node dissection for primary gastric tumors and R0 resection for liver metastases were achieved. The resected mass was confirmed to be poorly differentiated gastric carcinoma (hepatoid adenocarcinoma with neuroendocrine differentiation) with liver metastases in segments 6 and 8. A Follow-up review that began one month after surgery showed no recurrence or metastasis within the liver. The mother of three children miraculously lived a happy life and went back to work as normal. Conclusions: Application of ELRA in radical resection for GCLM can help selected patients with intrahepatic metastases located in complex sites obtain a favorable clinical outcome.
Baseline total metabolic tumor volume combined with international peripheral T-cell lymphoma project may improve prognostic stratification for patients with peripheral T-cell lymphoma (PTCL)
Authors: Jiang Chong , Teng Yue , Chen Jieyu , Wang Zhen , Zhou Zhengyang , Ding Chongyang , Xu Jingyan
Published: September 2020

Purpose: The aim of this study was to explore the prognostic value of total metabolic tumor volume (TMTV) at baseline 18F-FDG PET/CT in patients diagnosed with peripheral T-cell lymphoma (PTCL).Materials and methods: Eighty-four newly diagnosed PTCL patients who underwent baseline 18F-FDG PET/CT prior to treatment between March 2009 and January 2019 were enrolled in this retrospective study. The FDG-avid lesions in each patient were segmented using semiautomated software to calculate the maximum standardized uptake value (SUVmax), total metabolic tumor volume (TMTV), and total lesion glycolysis (TLG) values using the boundaries of voxels presenting with the 41% SUVmax threshold method. Progression-free survival (PFS) and overall survival (OS) were used as end points to evaluate patient prognosis. The log-rank test and Cox regression analyses were used to evaluate PFS and OS.Results: ROC curve analysis indicated an ideal TMTV cut-off value of 228.8 cm3. During the 4-131 month (29.2±28.5 months) follow-up period, high TMTV was significantly associated with worse PFS and OS. TMTV and the international peripheral T-cell lymphoma project score (IPTCLP) were independent predictors of PFS and OS with multivariate analysis. The combination of TMTV and the IPTCLP may provide significantly better risk substratification in PFS and OS of PTCL patients.Conclusions: Both TMTV and IPTCLP are independent predictors of PTCL patient survival outcomes. Moreover, the combination of TMTV and IPTCLP improved patient risk stratification and may contribute to personalized therapeutic regimens.
WITHDRAWN: An integrated process of methanol coagulation and side stream membrane bioreactor for treatment of rice gruel wastewater
Authors: Nazia Shaik , Mishra Karishma , Jegatheesan Veeriah , Bhargava Suresh K , Sundergopal Sridhar
Published: September 2020

Significant demand for water supply was to expect forthcoming decades. Production of reusable water from industrial and domestic wastewater is a feasible, cost-effective, and significant positive benefit to the environment. In the present study, the domestic rice gruel wastewater (RGW) treated with aqueous methanol coagulation integrated aerobic membrane bioreactor (AMBR). Hydrophilized spiral wound 5 kDa ultrafiltration membrane (HF – UF) used in the bioreactor for the effective removal of pollutants from RGW. Furthermore, the RGW pretreated with various coagulants such as methanol, ethanol, and HCl. The obtained pretreated RGW subjected to the HF-UF 5 kDa side stream AMBR for reusable water production. The experiments conducted to determine the turbidity, conductivity, total dissolved solids (TDS), and chemical oxygen demand (COD) found to be 96 %, 91 %, 91 %, and 94.6 %. The overall process was feasible, compact, environmentally free, cost-effective, eco-friendly.
MassARRAY-based single nucleotide polymorphism analysis in breast cancer of North Indian Population.
Authors: Bakshi Divya , Nagpal Ashna , Sharma Varun , Sharma Indu , Shah Ruchi , Sharma Bhanu , Bhat Amrita , Verma Sonali , Bhat Gh. Rasool , Abrol Deepak , Sharma Rahul , Vashnavi Samantha , Kumar Rakesh
Published: September 2020

Background Breast Cancer (BC) is associated with inherited gene mutations. High throughput genotyping of BC samples has led to the identification and characterization of biomarkers for the diagnosis of BC. The most common genetic variants studied are SNPs (Single Nucleotide Polymorphisms) that determine susceptibility to an array of diseases thus serving as a potential tool for identifying the underlying causes of breast carcinogenesis. Methods SNP genotyping employing the Agena MassARRAY offers a robust, sensitive, cost-effective method to assess multiple SNPs and samples simultaneously. In this present study, we analyzed 15 SNPs of 14 genes in 550 samples (150 cases and 400 controls). We identified four SNPs of genes TCF21, SLC19A1, DCC, and ERCC1 showing significant association with BC in the population under study. Results The SNPs were rs12190287 (TCF21) having OR 1.713 (1.08-2.716 at 95% CI) p-value 0.022 (dominant), rs1051266 (SLC19A1) having OR 3.461 (2.136-5.609 at 95% CI) p-value 0.000000466 (dominant), rs2229080 (DCC) having OR 0.6867 (0.5123 -0.9205 at 95% CI) p-value 0.0116 (allelic) and rs2298881 (ERCC1) having OR 0.669 (0.46-0.973 at 95% CI), p-value 0.035 (additive) respectively. The in-silico analysis was further used to fortify the above findings. Conclusion It is further anticipated that the variants should be evaluated in other population groups that may aid in understanding the genetic complexity and bridge the missing heritability.
Nutritional and Inflammatory Measures Predict Survival of Patients with Stage IV Colorectal Cancer
Authors: Takamizawa Yasuyuki , Shida Dai , Boku Narikazu , Nakamura Yuya , Ahiko Yuka , Yoshida Takefumi , Tanabe Taro , Takashima Atsuo , Kanemitsu Yukihide
Published: September 2020

Background: This study aimed to evaluate the prognostic impact of nutritional and inflammatory measures (controlling nutritional status (CONUT) score, prognostic nutritional index (PNI), and modified Glasgow prognostic score (mGPS)) on overall survival (OS) in patients with stage IV colorectal cancer (CRC).Methods: Subjects were 996 patients with stage IV CRC who were referred to the National Cancer Center Hospital between 2001 and 2015. We retrospectively investigated correlations between OS and CONUT score, PNI, and mGPS. Multivariate analyses were performed using Cox proportional hazards regression models.Results: After adjusting for known factors (age, gender, BMI, ECOG performance status, location of primary tumor, CEA levels, histological type, M category, and prior surgical treatment), all three measures were found to be independent prognostic factors for OS in patients with stage IV CRC (CONUT score, p<0.001; PNI, p<0.001; mGPS, p<0.001). Significant differences in OS were found between low CONUT score (0/1) (n=614; 61%) and intermediate CONUT score (2/3) (n=276; 28%) (hazard ratio (HR)=1.20, 95% confidence interval (CI): 1.02-1.42, p=0.032), and intermediate CONUT score and high CONUT score (≥4) (n=106; 11%) (HR=1.30, 95% CI: 1.01-1.67, p=0.045). Significant differences in OS were found between mGPS=0 (n=633; 64%) and mGPS=1 (n=234; 23%) (HR=1.84, 95% CI: 1.54-2.19, p<0.001), but not between mGPS=1 and mGPS=2 (n=129; 13%) (HR=1.12, 95% CI: 0.88-1.41, p=0.349). Patients with low PNI (<48.0) (n=443; 44%) showed a significantly lower OS rate than those with high PNI (≥48.0) (n=553; 56%) (HR=1.39, 95% CI: 1.19-1.62, p<0.001).Conclusions: CONUT score, PNI, and mGPS were found to be independent prognostic factors for OS in patients with stage IV CRC, suggesting that nutritional and inflammatory status is a useful host-related prognostic indicator in stage IV CRC.
Differences between encoding and retrieval failure in mild cognitive impairment: results from quantitative electroencephalography and magnetic resonance volumetry
Authors: Han Su-Hyun , Pyun Jungmin , Yeo Soeun , Kang Dong Won , Jeong Ho Tae , Kang Seung Wan , Kim SangYun , Youn Young Chul
Published: September 2020

Background: The memory impairments in mild cognitive impairment (MCI) can be classified into encoding (EF) and retrieval (RF) failure, which can be affected by underlying pathomechanism. We explored the differences structurally and functionally.Methods: We compared quantitative electroencephalography (qEEG) power spectra and connectivity between 87 MCI patients with EF and 78 MCI with RF using iSyncBrain ™ (iMediSync, Inc., Korea) (https://isyncbrain.com/). Voxel-based morphometric analysis of the gray matter (GM) in the MCI groups and 71 cognitive normal controls were also done using the Computational Anatomy Toolbox 12 (http://www.neuro.uni-jena.de/cat/).Results: qEEG showed higher frontal theta and lower beta2 band power and higher theta and lower beta connectivity in the EF. There was no statistically significant difference in GM volume between the EF and RF. However, when compared to normal control, GM volume reductions due to EF in the left thalamus and bilateral hippocampi and reductions due to RF in the left thalamus, right superior frontal lobe, right superior temporal lobe, and right middle cingulum were observed ( p < 0.05, family-wise error correction). Conclusions: MCI differs functionally and structurally according to their specific memory impairments. The EF findings are structurally and functionally more consistent with the prodromal Alzheimer's disease stage than the RF findings. Since this study is a cross-sectional study, prospective follow-up studies are needed to investigate whether different types of memory impairments can predict the underlying pathology of amnestic MCI. Additionally, insufficient sample size may lead to ambiguous statistical findings in direct comparisons, a larger patient cohort could more robustly identify differences in GM volume reductions between the EF and the RF group.
Ten years of research and publications about hospice and palliative care in Mainland China: a bibliometric study
Authors: Jin Shuang , Liu Shuo , Li Jiaojiao , Ning Xiaohong , Liu Xiaohong
Published: September 2020

Background

Research is a fundamental element in the sustainable development of hospice and palliative care. Mainland China is facing increasing demand for hospice and palliative care and has launched related policies over the past decade. However, the state of research and publications in this field in China remains largely unknown. This study aimed to provide an overall picture of hospice-and palliative care -related research and publications in Mainland China from 2010 to 2019.

Methods

We searched Web of Science, Scopus, PubMed, CINAHL, OVID, and China National Knowledge Infrastructure for hospice-and palliative care -related publications in English and Chinese for 2010–2019. We analyzed the production, citations and impacts, publishing journals, region and institution of origin, and themes and active topics.

Results

A total of 3224 publications were identified, and 636 of them were considered of high quality. The production and impacts showed a clear increase—especially after 2016. However, the regional disparity between East and Western China in production was conspicuous and closely linked to economic factors. Beijing and Shanghai were the most productive regions. The hospice providers in the first five pilot regions had no collaboration with leading universities in their publications. Hospice and palliative care for cancer patients was the most common publication topic; some essential themes were rarely explored.

Conclusion

Research and publications of hospice and palliative care in Mainland China is developing faster than before; however, it remains at an early stage and should be promoted in terms of interregional equity. Collaboration among different disciplines, institutes, and regions should be encouraged.
The interaction between TERT promoter mutation and MGMT promoter methylation on overall survival of glioma patients: a meta-analysis
Authors: Vuong Huy Gia , Nguyen Thu Quynh , Ngo Tam N.M , Nguyen Hoang Cong , Fung Kar-Ming , Dunn Ian F.
Published: September 2020

Background There are controversial results concerning the prognostic implication of TERT promoter mutation in glioma patients concerning MGMT status. In this meta-analysis, we investigated whether there are any interactions of these two genetic markers on the overall survival (OS) of glioma patients. Methods Electronic databases including PubMed and Web of Science were searched for relevant studies. Hazard ratio (HR) and its 95% confidence interval (CI) for OS adjusted for selected covariates were calculated from the individual patient data (IPD), Kaplan-Meier curve (KMC), or directly obtained from the included studies. Results A total of nine studies comprising 2819 glioma patients were included for meta-analysis. Our results showed that TERT promoter mutation was associated with a superior outcome in MGMT-methylated gliomas (HR = 0.73; 95% CI = 0.55-0.98; p-value = 0.04), whereas this mutation was associated with poorer survival in gliomas without MGMT methylation (HR = 1.86; 95% CI = 1.54-2.26; p-value < 0.001). TERT-mutated glioblastoma (GBM) patients with MGMT methylation benefited from temozolomide (TMZ) treatment (HR = 0.33; 95% CI = 0.23-0.47; p-value < 0.001). MGMT methylation was not related with any improvement in OS in TERT-wild type GBMs (HR = 0.80; 95% CI = 0.56-1.15; p-value = 0.23).ConclusionsThe prognostic value of TERT promoter mutation may be modulated by MGMT methylation status. Not all MGMT-methylated GBM patients may benefit from TMZ; it is possible that only TERT-mutated GBM with MGMT methylation, in particular, may respond.
Continuous Block at the Proximal End of the Adductor Canal Provides Better Analgesia Compared to that at the Middle of the Canal After Total Knee Arthroplasty: A Randomized, Double-blind, Controlled Trial
Authors: Fei Yuda , cui xulei , Chen Shaohui , Peng Huiming , Feng Bin , Qian Wenwei , Lin Jin , Weng Xisheng , Huang Yuguang
Published: September 2020

Background: The optimal position for continuous adductor canal block (ACB) for analgesia after total knee anthroplasty (TKA) remians controversial, mainly due to high variability in the localization of the the adductor canal (AC). Latest neuroanatomy studies show that the nerve to vastus medialis plays an important role in innervating the anteromedial aspect of the knee and dives outside of the exact AC at the proximal end of the AC. Therefore, we hypothesized that continuous ACB at the proximal end of the exact AC could provide a better analgesic effect after TKA compared with that at the middle of the AC (which appeared to only block the saphenous nerve). Methods: Sixty-two adult patients who were scheduled for a unilateral TKA were randomized to receive continuous ACB at the proximal end or middle of the AC. All patients received patient-controlled intravenous analgesia with sufentanil postoperatively. The primary outcome measure was cumulative sufentanil consumption within 24 h after the surgery, which was analyzed using Mann-Whitney U tests. P-values <0.05 (two-sided) were considered statistically significant. The secondary outcomes included postoperative sufentanil consumption at other time points, pain at rest and during passive knee flexion, quadriceps motor strength, and other recovery related paramaters.Results: Sixty patients eventually completed the study (30/group). The 24-h sufentanil consumption was 0.22 µg/kg (interquartile range [IQR]: 0.15–0.40 µg/kg) and 0.39 µg/kg (IQR: 0.23–0.52 µg/kg) in the proximal end and middle groups (P = 0.026), respectively. There were no significant inter-group differences in sufentanil consumption at other time points, pain at rest and during passive knee flexion, quadriceps motor strength, and other recovery related paramaters. Conclusions: Continuous ACB at the proximal end of the AC has a better opioid-sparing effect without a significant influence on quadriceps motor strength compared to that at the middle of the AC after TKA.These findings indicates that a true ACB may not produce the effective analgesia, instead, the proximal end AC might be a more suitable block to alleviate pain after TKA.Trial registration: This study was registered at ClinicalTrials.gov (NCT03942133; registration date: May 06, 2019; enrollment date: May 11, 2019).
Relationship between Household Food Insecurity and Food and Nutrition Literacy among Children of 9-12 years of age: a cross-sectional study in a city of Iran
Authors: Rouz Fatemeh Khorram , Doustmohammadian Azam , Eslami Omid , Khadem-Rezaiyan Majid , Pourmohammadi Parisa , Amini Maryam , Khosravi Maryam
Published: September 2020

ObjectiveThe aim of this study was to assess the relationship between household food insecurity (HFI) with food and nutrition literacy (FNLIT) in a sample of Iranian children. This cross sectional study was performed on 315 children aged 9 to 12 years recruited from the primary schools throughout Mashhad, Iran. The Household Food Insecurity Access Scale (HFIAS) was used to assess the HFI. Also, overall FNLIT score and its sub-categories were evaluated using a validated 40-item questionnaire.Results The prevalence of HFI in the total sample was about 56 %. Also, almost 14 % of students had a low FNLIT score. Food insecure children had significantly lower levels of FNLT and some subscales including nutrition knowledge, food choice literacy, and food label literacy than the food-secure subjects, moreover, they had a higher likelihood of having low FNLIT score (OR = 2.89, CI: 1.03-8.09; p = 0.04). In conclusion, there is a negative association between HFI and FNLIT in children. Further studies to confirm this finding are needed.
Elevated serum triglyceride predicates recurrence of colorectal polyps in patients with advanced adenomas
Authors: Liu Boying , Wen Pingwu , Gu Xiaodong , Weng Ruiqiang , Liu Sudong
Published: September 2020

Background: Recurrence of colorectal polyps is common and impacted by various factors. This study was performed to explore the association between lipid profiles and recurrence of colorectal polyps. Methods: This study retrospectively analyzed the lipid profiles of 435 patients who underwent colonoscopy with removal of colorectal polyps and assessed recurrence of polyps by follow-up colonoscopy. Multivariate regression logistic analysis was used to evaluate the association between lipid profiles and polyp recurrence. Results: During the 1.5-year follow-up, recurrence of colorectal polyps was observed in 135 of 435 patients (30.34%). Patients with recurrent polyps showed a higher level of triglycerides ( P = 0.006) and lower levels of high-density lipoprotein cholesterol ( P = 0.008) and apolipoprotein A1 ( P = 0.033). The multivariate regression logistic model suggested that an elevated triglyceride level was an independent risk factor for polyp recurrence (odds ratio, 1.55; 95% confidence interval, 1.02 - 2.35; P = 0.039) in patients with advanced adenoma. Conclusions: Lipid profiles are associated with recurrence of colorectal polyps. An elevated triglyceride level is an independent risk predictor of polyp recurrence in patients with advanced adenoma. Keywords: Colorectal polyp, Recurrence, Lipid profile, Triglyceride, Risk factor.
Echo Simulation of Dual Polarization Doppler Weather Radar Based on the Physical Model
Authors: JieLa Qutie , Wang Haijiang , Hu Shipeng , Zhu Jiahui , Gao Mengqing
Published: September 2020

Using the scattering characteristics of particles to simulate the radar echo can supply the test signals close to the real precipitation echo for the weather radar, and save the time and cost of the research and development and maintenance of the weather radar. In this paper, the precipitation echo of weather radar is simulated based on the theoretical basis that the falling raindrops have a shape well approximated by an oblate spheroid in the atmosphere. The Marshal-Palmer distribution is applied to describe the raindrop spectrum distribution of precipitation particles. It is assumed that the raindrop particles of different sizes have the random distribution in the radar resolution volume, and then the spatial distribution of precipitation particles in the resolution volume is modeled. The echo signals of horizontal and vertical polarization channels of dual polarization weather radar are obtained by vector superposition of backscattering echoes of each particle. The experimental results show that this method can describe the microphysical characteristics of precipitation particles more completely and can be used to test the signal processing module of dual polarization Doppler weather radar.
Large autologous ilium with periosteum for tibiotalar joint reconstruction in Rüedi-Allgöwer III or AO/OTA type C3 pilon fractures
Authors: Li Dong , Li Jiao Jiao , Zhu Yuanyuan , Hou Fushan , Li Yuan , Zhao Bin , Wang Bin
Published: September 2020

Background: Management of Rüedi-Allgöwer III or AO/OTA type C3 pilon fracture presents numerous challenges to the orthopaedic surgeon. A joint preservation technique using a large autologous ilium with periosteum in combination with internal implant fixation was reported to improve the outcome of reconstruction. Methods: Twenty-five patients according to Tscherne/Oestern FxCO-I closed fracture and FxOI open fractures classification after Rüedi-Allgöwer III or AO/OTA type C3 pilon fracture received a large autologous ilium with periosteum for tibiotalar joint reconstruction and open reduction and internal fixation (ORIF), between March 2015 and September 2018. The visual analog scale (VAS), American Orthopaedic Foot & Ankle Society (AOFAS) score, and Burwell and Charnley criteria were used for outcome analysis. Results: Twenty patients with an average age of 45.2 years were followed for an average of 18.3 months. The VAS and AOFAS scores, and Burwell and Charnley ratings were recorded at the last follow-up after reconstructive surgery. Two patients developed redness and swelling at the wound site, but recovered after local care and dressing changes. No patient displayed deep surgical site infection, donor site complication, non-union or local complication during the final follow-up. The average bone union time was 18.3 months (range 3-36). Conclusions: Large autologous ilium with periosteum in combination with ORIF can be performed for tibiotalar joint reconstruction. This procedure reduces the risk of post-operative complications following articular reconstruction for Rüedi-Allgöwer III or AO/OTA type C3 pilon fractures in short follow-up.Level of Evidence: Level III, retrospective cohort study
Association between polypharmacy and the persistence of delirium: a retrospective cohort study
Authors: Kurisu Ken , Miyabe Daisuke , Furukawa Yoshiko , Shibayama Osamu , Yoshiuchi Kazuhiro
Published: September 2020

Background: Although the association between polypharmacy and the occurrence of delirium has been well studied, the influence of polypharmacy on the persistence of delirium remains unclear. We aimed to explore the effect of polypharmacy on the persistence of delirium.Methods: This retrospective cohort study was conducted at a tertiary hospital. The medical records of patients diagnosed with delirium who were referred to the Department of Psychosomatic Medicine were reviewed. Presentation with delirium on day 3 was set as the outcome in this study. We counted the number of drugs prescribed on the date of referral, excluding general infusion fluids, nutritional or electrolytic products, and psychotropics. To define polypharmacy, we developed a classification and regression tree (CART) model and drew a receiver operating characteristic (ROC) curve. The odds ratio (OR) of polypharmacy for the persistence of delirium on day 3 was calculated using a logistic regression model with the propensity score as a covariate.Results: We reviewed the data of 113 patients. The CART model and ROC curve indicated an optimal polypharmacy cutoff of six drugs. Polypharmacy was significantly associated with the persistence of delirium both before [OR, 3.02; 95% confidence interval (CI), 1.39–6.81; P = 0.0062] and after (OR, 3.19; 95% CI, 1.32–8.03; P = 0.011) propensity score adjustment.Conclusion: We discovered an association between polypharmacy and worsening courses of delirium and hypothesize that polypharmacy might be a prognostic factor for delirium.
Is There Any Relation Between Insulin Resistance and Menstrual Irregularity in Obese Women?
Authors: Almassinokiani Fariba , Akbari Peyman , Soheilipour Fahimeh
Published: September 2020

Objectives: Menstrual irregularity is a common issue in obese women that can cause anovulation and infertility. Insulin resistance is also common in obese women. We aimed to find the relation between menstrual pattern and insulin resistance in obese women.Results: The samples were 233 obese women 18-36 years old coming to obesity clinic for treatment of obesity. We checked Homeostatic Model Assessment for Insulin Resistance (HOMA – IR) and lipid profile in the clients and also we filled a form including the age, educational status, marital status, menstrual pattern and severity of hirsutism. Sample size was 233. The mean age of women was 25.6±8 years. The mean Body mass index (BMI) was 44.6±4.8 kg/m2. 84.55% of women had insulin resistance. There was significant relation between menstrual pattern and fasting insulin level (p=0.05). There was no significant relation between presence of Insulin resistance and menstrual pattern (p=0.28). There was significant relation between age and menstrual pattern (p=0.007). Based on the results, there was no significant relation between HOMA- IR and menstrual pattern (p=0.28). Perhaps type of obesity (android) may have relation with menstrual pattern and also we need to a standard IR definition to explain the relation between different findings.
Genome sequence analysis of the beneficial Bacillus subtilis PTA-271 isolated from a Vitis vinifera (cv. Chardonnay) rhizospheric soil: assets for sustainable biocontrol.
Authors: LEAL Catarina , Fontaine Florence , Aziz Aziz , Egas Conceiçao , Clement Christophe , Trotel-Aziz Patricia
Published: September 2020

Background: Bacillus subtilis strains have been widely studied for their innumerous benefits in agriculture, including viticulture. Providing numerous assets, B. subtilis spp. are described as promising plant-protectors against many pathogens and as influencers to adaptations in a changing environment. This study reports the draft genome sequence of the beneficial Bacillus subtilis PTA-271, isolated from the rhizospheric soil of healthy Vitis vinifera cv. Chardonnay at Champagne Region in France, attempting to draw outlines of its full biocontrol capacity. Results: The PTA-271 genome has a size of 4,001,755 bp, with 43.78% of G + C content and 3,945 protein coding genes. The draft genome of PTA-271 highlights (1) a functional swarming motility system hypothesizing a colonizing capacity and a strong interacting capacity, (2) strong survival capacities and (3) a set of genes encoding for bioactive substances. Bioactive compounds are known both to (i) stimulate plant growth or defenses such as hormones and elicitors, (ii) influence beneficial microbiota, and (ii) counteract pathogen aggressiveness such as effectors and many kinds of detoxifying enzymes. Conclusions: The plurality of the encoded biomolecules by Bacillus subtilis PTA-271 genome appears as strengths for PTA-271 biocontrol potential towards plants, offering a big potential against a broad spectrum of pathogens despite environmental constraints.
Reuse of Improved Recycled Concrete Aggregates (RCA) for Sustainable and Environmental-Friendly Rigid Pavements.
Authors: Alyaseen Ahmad Sarhan , Shah Siddarth , Solanki Ravindra , Daxini Bhavik , Alwani Yogesh K.
Published: September 2020

Recycled aggregates have an important role to play in construction activities in the world today to save natural aggregates because of industrial development. The goal of the research is to assess the suitability of recycled aggregates for the construction of new roads, which will help to achieve the efficiency of road construction and also Assist to prevent environmental deterioration in the extraction and reducing pollution. In contrast with natural aggregates, recycled aggregates are of lower quality, mainly due to the brittle nature of the cement mortar attached to them. The point of the study is to increase the performance of RCAs in an environmentally friendly managing RCAs. In this process, RCAs are first soaked in acetic acid solution in which acetic acid reacts with cement attached to the surface of the RCA. This reaction weakens the attached mortar and allows separating from the RCAs by using mechanical friction later. Treated RCAs have lower water absorption and lower cement mortar adhesion. These RCAs that have been used as aggregates in new concrete, increased the compressive strength, the tensile strength, and the flexural strength of the concrete by 26%, 11% and 26% at 28 days, respectively. It is clean, safe, efficient, and a new method to be applied so no harmful products are used and no dangerous substances are incorporated into the RCAs that are being treated. The waste treatment solution was used as a solvent for fresh construction, increasing the strength of the concrete as well as decreasing its environmental effects.
Personalised health education against health damage of COVID-19 epidemic in the elderly hungarian population (PROACTIVE-19): protocol of an adaptive randomised controlled clinical trial.
Authors: Erőss Bálint , Molnár Zsolt , Szakács Zsolt , Zádori Noémi , Szakó Lajos , Váncsa Szilárd , Juhász Félix , Ocskay Klementina , Vörhendi Nóra , Márta Katalin , Szentesi Andrea , Párniczky Andrea , Hegyi Péter J. , Kiss Szabolcs , Földi Mária , Dembrovszky Fanni , Kanjo Anna , Pázmány Piroska , Varró András , Csathó Árpád , Helyes Zsuzsanna , Péterfi Zoltán , Czopf László , Kiss István , Zemplényi Antal , Czapári Dóra , Hegyi Eszter , Dobszai Dalma , Miklós Emőke , Márta Attila , Tóth Dominika , Farkas Richard , Farkas Nelli , Birkás Béla , Pintér Erika , Pethő Gábor , Zsigmond Borbála , Sárközi Andrea , Nagy Anikó , Hegyi Péter
Published: September 2020

Background: Early reports indicate that COVID-19 may require intensive care unit (ICU) admission in 5-26% and overall mortality can rise to 11% of the recognized cases, particularly affecting the elderly. There is a lack of evidence-based targeted pharmacological therapy for prevention and treatment alike.

We aim to compare the effects of a World Health Organisation (WHO) recommendations’ based education and a personalised complex preventive lifestyle intervention package (based on the same WHO recommendation) on the outcomes of the COVID-19.

Methods: PROACTIVE-19 is a pragmatic, randomized controlled clinical trial with adaptive “sample size re-estimation” design. Hungarian population over the age of 60 years without confirmed COVID-19 will be approached to participate in a telephone health assessment and lifestyle counselling voluntarily. Volunteers will be randomized into two groups: (A) general health education; (B) personalized health education. Participants will go through questioning and recommendation in 5 fields: (1) mental health, (2) smoking habits, (3) physical activity, (4) dietary habits, (5) alcohol consumption. Both groups A and B will receive the same line of questioning to assess habits concerning these topics. Assessment will be done weekly during the first month, every second week in the second month, then monthly. The composite primary endpoint will include the rate of ICU admission, hospital admission (longer han 48 hours) and mortality in COVID-19 positive cases. The estimated sample size is 3788 subjects per study arm. The planned duration of the follow-up is a minimum of one year.

Discussion: These interventions may boost the body’s cardiovascular and pulmonary reserve capacities, leading to improved resistance against the damage caused by COVID-19. Consequently, lifestyle changes can reduce the incidence of life-threatening conditions and attenuate the detrimental effects of the pandemic seriously affecting the older population.

Trial registration: The study has been approved by the Scientific and Research Ethics Committee of the Hungarian Medical Research Council (IV/2428- 2 /2020/EKU) and has been registered at clinicaltrials.gov (NCT04321928) on 25 March, 2020.