bluuebunny/crossref_metadata_2025 huggingface dataset:
Degradation Mechanism of Concrete Subjected to External Sulfate Attack: Comparison of Different Curing Conditions
Authors: Zhao Gaowen , Shi Mei , Guo Mengzhen , Fan Henghui
Published: July 2020

Sulfate induced degradation of concrete brings great damage to concrete structures in saline or offshore areas. The degradation mechanism of cast-in-situ concrete still remains unclear. This paper investigates the degradation process and corresponding mechanism of cast-in-situ concrete when immersed in sulfate-rich corrosive environments. Concrete samples with different curing conditions were prepared and immersed in sulfate solutions for 12 months to simulate the corrosion of precast and cast-in-situ concrete structures, respectively. Tests regarding the changes of physical, chemical, and mechanical properties of concrete samples were conducted and recorded continuously during the immersion. Micro-structural and mineral methods were performed to analyze the changes of concrete samples after immersion. Results indicate that the corrosion process of cast-in-situ concrete is much faster than the degradation of precast concrete. Chemical attack is the main cause of degradation for both precast and cast-in-situ concrete. Concrete in the environment with higher sulfate concentration suffers more severe degradation. The water/cement ratio has a significant influence on the durability of concrete. A lower water/cement ratio results in obviously better resistance against sulfate attack for both precast and cast-in-situ concrete.
Preclinical Study of a Multi-Layered Antimicrobial Patch Based on Thin Nanocomposite Amorphous Diamond Like Carbon Films with Embedded Silver Nanoparticles
Authors: Juknius Tadas , Juknienė Indrė , Tamulevičius Tomas , Ružauskas Modestas , Pamparienė Ina , Oberauskas Vaidas , Jurkevičiūtė Aušrinė , Vasiliauskas Andrius , Tamulevičius Sigitas
Published: July 2020

A growing number of severe infections are related to antibiotic-resistant bacteria, therefore, in recent years, alternative antimicrobial materials based on silver nanoparticles (NPs) attracted a lot of attention. In the current research, we present a medical patch prototype containing diamond-like carbon nanocomposite thin films doped with silver nanoparticles (DLC:Ag), as a source of silver ions, and an aqueous mass of the gelatin/agar mixture as a silver ion accumulation layer. The DLC:Ag thin films with 3.4 at.% of silver were deposited on synthetic silk employing reactive unbalanced DC magnetron sputtering of the silver target with argon ions performed in the acetylene gas atmosphere. The average size of the silver nanoparticles as defined by scanning electron microscope was 24 nm. After the film deposition, the samples were etched with RF oxygen plasma, aiming at efficient silver ion release in aqueous media from the nanocomposite film. In the patch prototype, a mixture of agar and gelatin was applied in silicone carrier with cavities, acting as a silver ion accumulation layer that further enhanced the antimicrobial efficiency. It was found that the DLC:Ag thin film on the silk after soaking in water for 24 h was able to release up to 4 ppm of Ag. The microbiological experiments using S. aureus bacteria were performed with the patch prototype and the silver ion saturated water, demonstrated the inactivation of 99% and 79% of bacteria, respectively. Scanning electron microscopy analysis showed that silver NPs destroyed the bacteria cell and the bacteria affected by Ag ions had spots and perforated cell wall areas with cytoplasm leakage out was obtained. A preliminary preclinical study using the laboratory animals demonstrated that using the patch prototype, the methicillin-resistant S. aureus (MRSA)-infected wound on skin surface healed faster compared with control and was able to kill all MRSA bacteria strains in the wound’s bed after 72 h of treatment.
Durability and Strength Characteristics of Casein-Cemented Sand with Slag
Authors: Park Sung-Sik , Woo Seung-Wook , Jeong Sueng-Won , Lee Dong-Eun
Published: July 2020

Casein is often used as an eco-friendly wood adhesive. In this study, we used casein for soil cementation by mixing it with Jumunjin sand, sodium hydroxide (SH), and calcium hydroxide (CH) as a standard casein formula. The modified casein binder with different proportions of SH and CH was applied to improve water resistance. Furthermore, a blast furnace slag (BFS) was additionally mixed and reacted with alkalinity of modified casein binder. Thus, three types (standard, modified, and modified + BFS, referred to as STD, MOD, and MBS, hereafter) of casein binders were tested for durability and strength of casein-cemented sand. A piezoelectric sensor was installed within each sample to determine the curing time of the casein-cemented samples. The samples were air-cured at room temperature for seven days and some were repeatedly immersed in water thrice. Unconfined compression and jar slake tests were carried out to evaluate the strength and durability of the casein-cemented sand. Also, the microstructure was analyzed using a scanning electron microscope (SEM). We observed variations of peak conductance and corresponding frequency converged as the curing time increased. It was most significant for the MBS samples, which developed strength early. The unconfined compressive strength (UCS) of the air-cured samples was higher than those repeatedly immersed in water due to wash-off of the casein binder. The UCS of the dry MBS sample was 9900 kPa while that of the immersed sample was 430 kPa, which gradually decreased to 60 kPa upon repeated immersion. The samples with STD and MOD had no resistance to durability and showed cracks on the surface, while the MBS sample exhibited significantly improved durability and no cracks. We found that the MBS binder had a positively significant effect on the durability and strength of casein-cemented sand.
Influence of Anodization Temperature on Geometrical and Optical Properties of Porous Anodic Alumina(PAA)-Based Photonic Structures
Authors: Białek Ewelina , Włodarski Maksymilian , Norek Małgorzata
Published: July 2020

In this work, the influence of a wide range anodizing temperature (5–30 °C) on the growth and optical properties of PAA-based distributed Bragg reflector (DBR) was studied. It was demonstrated that above 10 °C both structural and photonic properties of the DBRs strongly deteriorates: the photonic stop bands (PSBs) decay, broaden, and split, which is accompanied by the red shift of the PSBs. However, at 30 °C, new bands in transmission spectra appear including one strong and symmetric peak in the mid-infrared (MIR) spectral region. The PSB in the MIR region is further improved by a small modification of the pulse sequence which smoothen and sharpen the interfaces between consecutive low and high refractive index layers. This is a first report on PAA-based DBR with a good quality PSB in MIR. Moreover, it was shown that in designing good quality DBRs a steady current recovery after subsequent application of high potential (UH) pulses is more important than large contrast between low and high potential pulses (UH-UL contrast). Smaller UH-UL contrast helps to better control the current evolution during pulse anodization. Furthermore, the lower PSB intensity owing to the smaller UH-UL contrast can be partially compensated by the higher anodizing temperature.
A Weak Convergence Self-Adaptive Method for Solving Pseudomonotone Equilibrium Problems in a Real Hilbert Space
Authors: Yordsorn Pasakorn , Kumam Poom , Rehman Habib ur , Hassan Ibrahim Abdulkarim
Published: July 2020

In this paper, we presented a modification of the extragradient method to solve pseudomonotone equilibrium problems involving the Lipschitz-type condition in a real Hilbert space. The method uses an inertial effect and a formula for stepsize evaluation, that is updated for each iteration based on some previous iterations. The key advantage of the algorithm is that it is achieved without previous knowledge of the Lipschitz-type constants and also without any line search procedure. A weak convergence theorem for the proposed method is well established by assuming mild cost bifunction conditions. Many numerical experiments are presented to explain the computational performance of the method and to equate them with others.
Investigation of a Multitasking System for Automatic Ship Berthing in Marine Practice Based on an Integrated Neural Controller
Authors: Nguyen Van Suong
Published: July 2020

In this article, a multitasking system is investigated for automatic ship berthing in marine practices, based on artificial neural networks (ANNs). First, a neural network with separate structures in hidden layers is developed, based on a head-up coordinate system. This network is trained once with the berthing data of a ship in an original port to conduct berthing tasks in different ports. Then, on the basis of the developed network, an integrated mechanism including three negative signs is linked to achieve an integrated neural controller. This controller can bring the ship to a berth on each side of the ship in different ports. The whole system has the ability to berth for different tasks without retraining the neural network. Finally, to validate the effectiveness of the proposed system for automatic ship berthing, numerical simulations were performed for berthing tasks, such as different ports, and berthing each side of the ship. The results indicate that the proposed system shows a good performance in automatic ship berthing.
Hemodynamic Effects of Alpha-Tropomyosin Mutations Associated with Inherited Cardiomyopathies: Multiscale Simulation
Authors: Syomin Fyodor , Khabibullina Albina , Osepyan Anna , Tsaturyan Andrey
Published: July 2020

The effects of two cardiomyopathy-associated mutations in regulatory sarcomere protein tropomyosin (Tpm) on heart function were studied with a new multiscale model of the cardiovascular system (CVS). They were a Tpm mutation, Ile284Val, associated with hypertrophic cardiomyopathy (HCM), and an Asp230Asn one associated with dilated cardiomyopathy (DCM). When the molecular and cell-level changes in the Ca2+ regulation of cardiac muscle caused by these mutations were introduced into the myocardial model of the left ventricle (LV) while the LV shape remained the same as in the model of the normal heart, the cardiac output and arterial blood pressure reduced. Simulations of LV hypertrophy in the case of the Ile284Val mutation and LV dilatation in the case of the Asp230Asn mutation demonstrated that the LV remodeling partially recovered the stroke volume and arterial blood pressure, confirming that both hypertrophy and dilatation help to preserve the LV function. The possible effects of changes in passive myocardial stiffness in the model according to data reported for HCM and DCM hearts were also simulated. The results of the simulations showed that the end-systolic pressure–volume relation that is often used to characterize heart contractility strongly depends on heart geometry and cannot be used as a characteristic of myocardial contractility.
Evaluation of the Probiotic Properties and the Capacity to Form Biofilms of Various Lactobacillus Strains
Authors: Chamignon Célia , Guéneau Virgile , Medina Sonia , Deschamps Julien , Gil-Izquierdo Angel , Briandet Romain , Mousset Pierre-Yves , Langella Philippe , Lafay Sophie , Bermúdez-Humarán Luis G.
Published: July 2020

Over the last 20 years, Lactobacillus species inhabiting the gastrointestinal tract (GIT) have received much attention, and their health-promoting properties are now well-described. Probiotic effects cannot be generalized, and their uses cover a wide range of applications. It is thus important to proceed to an accurate selection and evaluation of probiotic candidates. We evaluate the probiotic potential of six strains of Lactobacillus in different in vitro models representing critical factors of either survival, efficacy, or both. We characterized the strains for their ability to (i) modulate intestinal permeability using transepithelial electrical resistance (TEER), (ii) form biofilms and resist stressful conditions, and (iii) produce beneficial host and/or bacteria metabolites. Our data reveal the specificity of Lactobacillus strains to modulate intestinal permeability depending on the cell type. The six isolates were able to form spatially organized biofilms, and we provide evidence that the biofilm form is beneficial in a strongly acidic environment. Finally, we demonstrated the ability of the strains to produce γ-aminobutyric acid (GABA) that is involved in the gut-brain axis and beneficial enzymes that promote the bacterial tolerance to bile salts. Overall, our study highlights the specific properties of Lactobacillus strains and their possible applications as biofilms.
Phytophthora Diversity in Pennsylvania Nurseries and Greenhouses Inferred from Clinical Samples Collected over Four Decades
Authors: Molnar Cody , Nikolaeva Ekaterina , Kim Seonghwan , Olson Tracey , Bily Devin , Kim Jung-Eun , Kang Seogchan
Published: July 2020

The increasing movement of exotic pathogens calls for systematic surveillance so that newly introduced pathogens can be recognized and dealt with early. A resource crucial for recognizing such pathogens is knowledge about the spatial and temporal diversity of endemic pathogens. Here, we report an effort to build this resource for Pennsylvania (PA) by characterizing the identity and distribution of Phytophthora species isolated from diverse plant species in PA nurseries and greenhouses. We identified 1137 Phytophthora isolates cultured from clinical samples of >150 plant species submitted to the PA Department of Agriculture for diagnosis from 1975 to 2019 using sequences of one or more loci and morphological characteristics. The three most commonly received plants were Abies, Rhododendron, and Pseudotsuga. Thirty-six Phytophthora species identified represent all clades, except 3 and 10, and included a distinct subgroup of a known species and a prospective new species. Prominent pathogenic species such as P. cactorum, P. cinnamomi, P. nicotianae, P. drechsleri, P. pini, P. plurivora, and P. sp. kelmania have been found consistently since 1975. One isolate cultured from Juniperus horizontalis roots did not correspond to any known species, and several other isolates also show considerable genetic variation from any authentic species or isolate. Some species were isolated from never-before-documented plants, suggesting that their host range is larger than previously thought. This survey only provides a coarse picture of historical patterns of Phytophthora encounters in PA nurseries and greenhouses because the isolation of Phytophthora was not designed for a systematic survey. However, its extensive temporal and plant coverage offers a unique insight into the association of Phytophthora with diverse plants in nurseries and greenhouses.
Characterization of Clostridium tyrobutyricum Strains Using Three Different Typing Techniques
Authors: Burtscher Johanna , Küller Franziska , Dreier Matthias , Arias-Roth Emmanuelle , Drissner David , Domig Konrad J.
Published: July 2020

Clostridium tyrobutyricum is well known as one of the main causative agents of severe cheese spoilage. The metabolism of this anaerobic bacterium during ripening leads to textural and sensory defects in cheese and consequential loss of product value. The potential to induce cheese spoilage, however, may vary among different strains of the same species. Therefore, a better understanding of the intra-species diversity of C. tyrobutyricum may be of practical relevance for the dairy industry. In the present study, we compared the ability of three typing techniques to differentiate 95 C. tyrobutyricum strains on the subspecies level: (1) repetitive element palindromic PCR (rep-PCR) fingerprinting combined with conventional agarose gel electrophoresis, (2) hexaplex-PCR followed by an automated capillary electrophoresis and (3) matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) typing. MALDI-TOF MS fingerprinting provided only moderate reproducibility and low discriminatory power. Both PCR-based methods were highly reproducible and discriminative, with hexaplex-PCR fingerprinting being slightly more discriminative than rep-PCR typing. Overall, a high intra-species diversity was observed among the tested strains, indicating that further investigations on the strain level may be of interest.
Lung Microbiome Participation in Local Immune Response Regulation in Respiratory Diseases
Authors: Lira-Lucio Juan Alberto , Falfán-Valencia Ramcés , Ramírez-Venegas Alejandra , Buendía-Roldán Ivette , Rojas-Serrano Jorge , Mejía Mayra , Pérez-Rubio Gloria
Published: July 2020

The lung microbiome composition has critical implications in the regulation of innate and adaptive immune responses. Next-generation sequencing techniques have revolutionized the understanding of pulmonary physiology and pathology. Currently, it is clear that the lung is not a sterile place; therefore, the investigation of the participation of the pulmonary microbiome in the presentation, severity, and prognosis of multiple pathologies, such as asthma, chronic obstructive pulmonary disease, and interstitial lung diseases, contributes to a better understanding of the pathophysiology. Dysregulation of microbiota components in the microbiome–host interaction is associated with multiple lung pathologies, severity, and prognosis, making microbiome study a useful tool for the identification of potential therapeutic strategies. This review integrates the findings regarding the activation and regulation of the innate and adaptive immune response pathways according to the microbiome, including microbial patterns that could be characteristic of certain diseases. Further studies are required to verify whether the microbial profile and its metabolites can be used as biomarkers of disease progression or poor prognosis and to identify new therapeutic targets that restore lung dysbiosis safely and effectively.
Limited Response of Indigenous Microbes to Water and Nutrient Pulses in High-Elevation Atacama Soils: Implications for the Cold–Dry Limits of Life on Earth
Authors: Vimercati Lara , Bueno de Mesquita Clifton P. , Schmidt Steven K.
Published: July 2020

Soils on the world’s highest volcanoes in the Atacama region represent some of the harshest ecosystems yet discovered on Earth. Life in these environments must cope with high UV flux, extreme diurnal freeze–thaw cycles, low atmospheric pressure and extremely low nutrient and water availability. Only a limited spectrum of bacterial and fungal lineages seems to have overcome the harshness of this environment and may have evolved the ability to function in situ. However, these communities may lay dormant for most of the time and spring to life only when enough water and nutrients become available during occasional snowfalls and aeolian depositions. We applied water and nutrients to high-elevation soils (5100 meters above sea level) from Volcán Llullaillaco, both in lab microcosms and in the field, to investigate how microbial communities respond when resource limitations are alleviated. The dominant taxon in these soils, the extremophilic yeast Naganishia sp., increased in relative sequence abundance and colony-forming unit counts after water + nutrient additions in microcosms, and marginally in the field after only 6 days. Among bacteria, only a Noviherbaspirillum sp. (Oxalobacteraceae) significantly increased in relative abundance both in the lab and field in response to water addition but not in response to water and nutrients together, indicating that it might be an oligotroph uniquely suited to this extreme environment. The community structure of both bacteria and eukaryotes changed significantly with water and water + nutrient additions in the microcosms and taxonomic richness declined with amendments to water and nutrients. These results indicate that only a fraction of the detected community is able to become active when water and nutrients limitations are alleviated in lab microcosms, and that water alone can dramatically change community structure. Our study sheds light on which extremophilic organisms are likely to respond when favorable conditions occur in extreme earthly environments and perhaps in extraterrestrial environments as well.
A Method to Predict Water Recovery Rate in the Collection and Froth Zone of Flotation Systems
Authors: Martinez Jose , Maldonado Miguel , Gutierrez Leopoldo
Published: July 2020

This paper describes a method to predict water recovery rate into and through the foam in a bubble column operating under different gas rates, froth depths, and frother types and concentrations. Three frothers were considered: Metil Isobutil Carbinol (MIBC), a proprietary blend of alcohols, aldehydes, and esters commercialized under the name PINNACLE® 9891, and a PGE-based Dow Froth 1012 (DF1012). The water rate entering into the froth (foam) layer from the bubbly (collection) zone was estimated as the water rate overflowing the column when operating at a thin stable foam layer, i.e., 0.5 cm. It was observed that the rate at which water entered into the froth phase could be modelled as a unique linear function of the gas holdup below the froth, regardless of the frother chemistry. This is a fundamental result not previously found in the literature that also facilitates the calculation of the froth zone water recovery for deeper froths. The water recovery in the froth was found to be an inverse logarithmic function of the average liquid residence time in the froth. Although the same trend was observed for the three frothers tested, they did not converge into a single function, which suggests that frother chemistry plays a role in determining froth structure and then needs to be incorporated when modeling water transport in the froth. Finally, the water overflow rate calculated as the product of the water rate into the froth and froth water recovery predicted the actual measured values fairly well. The water transport model here proposed provides a simple representation of the interactions between collection and froth zone and its relation to easily measure operating variables.
Role of the Down-Bending Plate as a Detrital Source in Convergent Systems Revealed by U–Pb Dating of Zircon Grains: Insights from the Southern Andes and Western Italian Alps
Authors: Di Giulio Andrea , Amadori Chiara , Mueller Pierre , Langone Antonio
Published: July 2020

In convergent zones, several parts of the geodynamic system (e.g., continental margins, back-arc regions) can be deformed, uplifted, and eroded through time, each of them potentially delivering clastic sediments to neighboring basins. Tectonically driven events are mostly recorded in syntectonic clastic systems accumulated into different kinds of basins: trench, fore-arc, and back-arc basins in subduction zones and foredeep, thrust-top, and episutural basins in collisional settings. The most widely used tools for provenance analysis of synorogenic sediments and for unraveling the tectonic evolution of convergent zones are sandstone petrography and U–Pb dating of detrital zircon. In this paper, we present a comparison of previously published data discussing how these techniques are used to constrain provenance reconstructions and contribute to a better understanding of the tectonic evolution of (i) the Cretaceous transition from extensional to compressional regimes in the back-arc region of the southern Andean system; and (ii) the involvement of the passive European continental margin in the Western Alps subduction system during impending Alpine collision. In both cases, sediments delivered from the down-bending continental block are significantly involved. Our findings highlight its role as a detrital source, which is generally underestimated or even ignored in current tectonic models.
Selenium Deficiency Is Associated with Mortality Risk from COVID-19
Authors: Moghaddam Arash , Heller Raban , Sun Qian , Seelig Julian , Cherkezov Asan , Seibert Linda , Hackler Julian , Seemann Petra , Diegmann Joachim , Pilz Maximilian , Bachmann Manuel , Minich Waldemar , Schomburg Lutz
Published: July 2020

SARS-CoV-2 infections underlie the current coronavirus disease (COVID-19) pandemic and are causative for a high death toll particularly among elderly subjects and those with comorbidities. Selenium (Se) is an essential trace element of high importance for human health and particularly for a well-balanced immune response. The mortality risk from a severe disease like sepsis or polytrauma is inversely related to Se status. We hypothesized that this relation also applies to COVID-19. Serum samples (n = 166) from COVID-19 patients (n = 33) were collected consecutively and analyzed for total Se by X-ray fluorescence and selenoprotein P (SELENOP) by a validated ELISA. Both biomarkers showed the expected strong correlation (r = 0.7758, p < 0.001), pointing to an insufficient Se availability for optimal selenoprotein expression. In comparison with reference data from a European cross-sectional analysis (EPIC, n = 1915), the patients showed a pronounced deficit in total serum Se (mean ± SD, 50.8 ± 15.7 vs. 84.4 ± 23.4 µg/L) and SELENOP (3.0 ± 1.4 vs. 4.3 ± 1.0 mg/L) concentrations. A Se status below the 2.5th percentile of the reference population, i.e., [Se] < 45.7 µg/L and [SELENOP] < 2.56 mg/L, was present in 43.4% and 39.2% of COVID samples, respectively. The Se status was significantly higher in samples from surviving COVID patients as compared with non-survivors (Se; 53.3 ± 16.2 vs. 40.8 ± 8.1 µg/L, SELENOP; 3.3 ± 1.3 vs. 2.1 ± 0.9 mg/L), recovering with time in survivors while remaining low or even declining in non-survivors. We conclude that Se status analysis in COVID patients provides diagnostic information. However, causality remains unknown due to the observational nature of this study. Nevertheless, the findings strengthen the notion of a relevant role of Se for COVID convalescence and support the discussion on adjuvant Se supplementation in severely diseased and Se-deficient patients.
The Impact of Protein Supplementation Targeted at Improving Muscle Mass on Strength in Cancer Patients: A Scoping Review
Authors: Zanetti Michela , Gortan Cappellari Gianluca , Barazzoni Rocco , Sanson Gianfranco
Published: July 2020

Deterioration of muscle strength during cancer results in functional limitation, poor quality of life and reduced survival. The indirect effects on muscle strength of nutritional interventions based on protein and amino acid derivatives targeted at improving muscle mass are poorly documented. A scoping review was performed to examine the available evidence on the effects of proteins, amino acids and their derivatives on muscle strength in adult cancer patients. Pubmed and Scopus databases were searched to identify research articles published in the last 10 years. Fourteen studies met the inclusion criteria, showing that changes in muscle strength following protein or amino acid supplementation are generally concordant with those in muscle mass in cancer patients. Administration of both energy and proteins in the presence of reduced oral intakes results in more robust effects on both muscle strength and mass. It is not clear whether this is due to the correction of the energy deficit or to an interaction between proteins and other macronutrients. The optimal mixture, type, and dose of amino acid/protein supplementation alone or in combination with other anabolic strategies should be determined to provide the best nutritional approach in cancer.
Natural Variation of Hazelnut Allergenicity: Is There Any Potential for Selecting Hypoallergenic Varieties?
Authors: Ribeiro Miguel , Costa Joana , Mafra Isabel , Cabo Sandra , Silva Ana Paula , Gonçalves Berta , Hillion Mélanie , Hébraud Michel , Igrejas Gilberto
Published: July 2020

Hazelnuts (Corylus avellana L.) have an important role in human nutrition and health. However, they are a common cause of food allergy. Due to hazelnut varietal diversity, variety-dependent differences in the IgE-binding properties may be suspected, which could allow therapeutic strategies based on the use of hypoallergenic varieties to induce desensitization. In a proteogenomic approach, we aimed to evaluate the allergenic potential of a genetically diverse set of hazelnuts (n = 13 varieties). Minor differences were found at the level of genes encoding important allergens, namely Cor a 8, Cor a 9, and Cor a 14. Nevertheless, IgE-reactivity was similar for all varieties using sera from seven allergic individuals. The predominant IgE-reactive proteins were Cor a 9 (100%) and Cor a 1.04 (60%), with the former being the most frequently identified by a two-dimensional gel electrophoresis (2-DE)-based proteomic approach. Therefore, it seems that the conventional exclusion diet will hold its ground for the time being.
Caffeine Supplementation for 4 Days Does Not Induce Tolerance to the Ergogenic Effects Promoted by Acute Intake on Physiological, Metabolic, and Performance Parameters of Cyclists: A Randomized, Double-Blind, Crossover, Placebo-Controlled Study
Authors: Morales Anderson Pontes , Sampaio-Jorge Felipe , Barth Thiago , Pierucci Anna Paola Trindade Rocha , Ribeiro Beatriz Gonçalves
Published: July 2020

The present study investigated whether the caffeine supplementation for four days would induce tolerance to the ergogenic effects promoted by acute intake on physiological, metabolic, and performance parameters of cyclists. A double-blind placebo-controlled cross-over design was employed, involving four experimental trials; placebo (4-day)-placebo (acute)/PP, placebo (4-day)-caffeine (acute)/PC, caffeine (4-day)-caffeine (acute)/CC and caffeine (4-day)-placebo (acute)/CP. Fourteen male recreationally-trained cyclists ingested capsules containing either placebo or caffeine (6 mg∙kg−1) for 4 days. On day 5 (acute), capsules containing placebo or caffeine (6 mg∙kg−1) were ingested 60 min before completing a 16 km time-trial (TT). CC and PC showed improvements in time (3.54%, ES = 0.72; 2.53%, ES = 0.51) and in output power (2.85%, ES = 0.25; 2.53%, ES = 0.20) (p < 0.05) compared to CP and PP conditions, respectively. These effects were accompanied by increased heart rate (2.63%, ES = 0.47; 1.99%, ES = 0.34), minute volume (13.11%, ES = 0.61; 16.32%, ES = 0.75), expired O2 fraction (3.29%, ES = 0.96; 2.87, ES = 0.72), lactate blood concentration (immediately after, 29.51% ES = 0.78; 28.21% ES = 0.73 recovery (10 min), 36.01% ES = 0.84; 31.22% ES = 0.81), and reduction in expired CO2 fraction (7.64%, ES = 0.64; 7.75%, ES = 0.56). In conclusion, these results indicate that caffeine, when ingested by cyclists in a dose of 6 mg∙kg−1 for 4 days, does not induce tolerance to the ergogenic effects promoted by acute intake on physiological, metabolic, and performance parameters.
Associations of Urinary Phytoestrogen Concentrations with Sleep Disorders and Sleep Duration among Adults
Authors: Sun Jing , Jiang Hong , Wang Weijing , Dong Xue , Zhang Dongfeng
Published: July 2020

Current evidence on the relationship of phytoestrogens with sleep is limited and contradictory. In particular, studies on individual phytoestrogens and sleep have not been reported. Thus, this study aimed to appraise the associations of individual phytoestrogens with sleep disorders and sleep duration. This cross-sectional study comprising 4830 adults utilized data from the National Health and Nutrition Examination Survey 2005–2010. Phytoestrogens were tested in urine specimens. Sleep disorders and sleep duration were based on a self-reported doctor’s diagnosis and usual sleep duration. The main analyses utilized logistic and multinomial logistic regression models and a restricted cubic spline. In the fully adjusted model, compared with tertile 1 (lowest), the odds ratios (95% confidence intervals (CIs)) of sleep disorders for the highest tertile of urinary concentrations of enterolactone, enterodiol, and O-desmethylangolensin were 0.64 (0.41–1.00), 1.54 (1.07–2.21), and 1.89 (1.26–2.85), respectively. Linear inverse, approximatively linear positive, and inverted L-shaped concentration–response relationships were found between enterolactone, enterodiol, and O-desmethylangolensin and sleep disorders, respectively. Compared with normal sleep (7–8 h/night), the relative risk ratio (RRR) (95% CI) of very short sleep for enterolactone was 0.56 (0.36–0.86), and the RRR (95% CI) of long sleep risk for genistein was 0.62 (0.39–0.99). Furthermore, negative associations of genistein with sleep disorders and enterolactone with long sleep risk, as well as positive associations of enterodiol with both long and very short sleep, were observed in the stratified analysis by age or gender. Finally, a notable finding was that urinary O-desmethylangolensin concentration was positively related to sleep disorders in both females aged 40–59 years and non-Hispanic Whites but inversely associated with sleep disorders in both females aged 60 years or over and other Hispanics. Our findings suggested that enterolactone and genistein might be beneficial for preventing sleep disorders or non-normal sleep duration among adults, and enterodiol might be adverse toward this goal. However, the association of O-desmethylangolensin with sleep disorders might be discrepant in different races and females of different ages.
Vector Competence of Aedes aegypti, Aedes albopictus and Culex quinquefasciatus from Brazil and New Caledonia for Three Zika Virus Lineages
Authors: Fernandes Rosilainy S. , O’Connor Olivia , Bersot Maria Ignez L. , Girault Dominique , Dokunengo Marguerite R. , Pocquet Nicolas , Dupont-Rouzeyrol Myrielle , Lourenço-de-Oliveira Ricardo
Published: July 2020

Zika virus (ZIKV) has caused severe epidemics in South America beginning in 2015, following its spread through the Pacific. We comparatively assessed the vector competence of ten populations of Aedes aegypti and Ae. albopictus from Brazil and two of Ae. aegypti and one of Culex quinquefasciatus from New Caledonia to transmit three ZIKV isolates belonging to African, Asian and American lineages. Recently colonized mosquitoes from eight distinct sites from both countries were orally challenged with the same viral load (107 TCID50/mL) and examined after 7, 14 and 21 days. Cx. quinquefasciatus was refractory to infection with all virus strains. In contrast, although competence varied with geographical origin, Brazilian and New Caledonian Ae. aegypti could transmit the three ZIKV lineages, with a strong advantage for the African lineage (the only one reaching saliva one-week after challenge). Brazilian Ae. albopictus populations were less competent than Ae. aegypti populations. Ae. albopictus generally exhibited almost no transmission for Asian and American lineages, but was efficient in transmitting the African ZIKV. Viral surveillance and mosquito control measures must be strengthened to avoid the spread of new ZIKV lineages and minimize the transmission of viruses currently circulating.
Prevalence of Infectious Spleen and Kidney Necrosis Virus (ISKNV), Nervous Necrosis Virus (NNV) and Ectoparasites in Juvenile Epinephelus spp. Farmed in Aceh, Indonesia
Authors: Sah Putra Bakhtiar , Hick Paul M. , Hall Evelyn , Whittington Richard J. , Khairul Razi , Evarianti  , Nurbariah  , Becker Joy A.
Published: July 2020

A cross-sectional survey was used to estimate the prevalence of infections with the Infectious spleen and kidney necrosis virus (ISKNV, Megalocytivirus), nervous necrosis virus (NNV, Betanodavirus), and infestations with ectoparasites during the rainy season in juvenile grouper (Epinephelus spp.) farmed in Aceh, Indonesia. The survey was intended to detect aquatic pathogens present at 10% prevalence with 95% confidence, assuming 100% sensitivity and specificity using a sample size of 30 for each diagnostic test. Eight populations of grouper from seven farms were sampled. Additional targeted sampling was conducted for populations experiencing high mortality. Infection with NNV was detected at all farms with seven of the eight populations being positive. The apparent prevalence for NNV ranged from 0% (95% CI: 0–12) to 73% (95% CI: 54–88). All of the fish tested from the targeted samples (Populations 9 and 10) were positive for NNV and all had vacuolation of the brain and retina consistent with viral nervous necrosis (VNN). Coinfections with ISKNV were detected in five populations, with the highest apparent prevalence being 13% (95% CI: 4–31%). Trichodina sp., Cryptocaryon irritans and Gyrodactylus sp. were detected at three farms, with 66% to 100% of fish being infested. Hybrid grouper sourced from a hatchery were 5.4 and 24.9 times more likely to have a NNV infection and a higher parasite load compared to orange-spotted grouper collected from the wild (p < 0.001). This study found that VNN remains a high-impact disease in grouper nurseries in Aceh, Indonesia.
LiDAR-Aided Interior Orientation Parameters Refinement Strategy for Consumer-Grade Cameras Onboard UAV Remote Sensing Systems
Authors: Zhou Tian , Hasheminasab Seyyed Meghdad , Ravi Radhika , Habib Ayman
Published: July 2020

Unmanned aerial vehicles (UAVs) are quickly emerging as a popular platform for 3D reconstruction/modeling in various applications such as precision agriculture, coastal monitoring, and emergency management. For such applications, LiDAR and frame cameras are the two most commonly used sensors for 3D mapping of the object space. For example, point clouds for the area of interest can be directly derived from LiDAR sensors onboard UAVs equipped with integrated global navigation satellite systems and inertial navigation systems (GNSS/INS). Imagery-based mapping, on the other hand, is considered to be a cost-effective and practical option and is often conducted by generating point clouds and orthophotos using structure from motion (SfM) techniques. Mapping with photogrammetric approaches requires accurate camera interior orientation parameters (IOPs), especially when direct georeferencing is utilized. Most state-of-the-art approaches for determining/refining camera IOPs depend on ground control points (GCPs). However, establishing GCPs is expensive and labor-intensive, and more importantly, the distribution and number of GCPs are usually less than optimal to provide adequate control for determining and/or refining camera IOPs. Moreover, consumer-grade cameras with unstable IOPs have been widely used for mapping applications. Therefore, in such scenarios, where frequent camera calibration or IOP refinement is required, GCP-based approaches are impractical. To eliminate the need for GCPs, this study uses LiDAR data as a reference surface to perform in situ refinement of camera IOPs. The proposed refinement strategy is conducted in three main steps. An image-based sparse point cloud is first generated via a GNSS/INS-assisted SfM strategy. Then, LiDAR points corresponding to the resultant image-based sparse point cloud are identified through an iterative plane fitting approach and are referred to as LiDAR control points (LCPs). Finally, IOPs of the utilized camera are refined through a GNSS/INS-assisted bundle adjustment procedure using LCPs. Seven datasets over two study sites with a variety of geomorphic features are used to evaluate the performance of the developed strategy. The results illustrate the ability of the proposed approach to achieve an object space absolute accuracy of 3–5 cm (i.e., 5–10 times the ground sampling distance) at a 41 m flying height.
Intraseasonal Vertical Cloud Regimes Based on CloudSat Observations over the Tropics
Authors: Hung Meng-Pai , Chen Wei-Ting , Wu Chien-Ming , Chen Peng-Jen , Feng Pei-Ning
Published: July 2020

This study identifies the evolution of tropical vertical cloud regimes (CRs) and their associated heating structures on the intraseasonal time scales. Using the cloud classification retrievals of CloudSat during boreal winter between 2006 and 2017, the CR index is defined as the leading pair of the combined multivariate empirical orthogonal functions of the daily mean frequency of deep, high, and low clouds over the tropical Indian Ocean, Maritime Continents, and the Western Pacific. The principal components of the CR index exhibit robust temporal variance in the 30 to 80 day intraseasonal band. Based on the propagation stages of the CRs, the coherent vertical structures of cloud composition and large-scale moisture and vertical motion exhibit a westward-tilted structure. The associated Q1-QR diabatic heating and cloud radiative forcing are consistent with the key characteristics of the Madden Julian Oscillation (MJO) documented in the previous studies. Lastly, an MJO case study showcases that the presented approach characteristically captures the propagation of moisture, cloud vertical structure, and precipitation activity across spatial and temporal scales. The current results suggest that the CR index can potentially serve as an evaluation metric to cloud-associated processes in the simulated tropical intraseasonal variability in global climate models.
Detection of Very Small Tree Plantations and Tree-Level Characterization Using Open-Access Remote-Sensing Databases
Authors: Alonso Laura , Picos Juan , Bastos Guillermo , Armesto Julia
Published: July 2020

Highly fragmented land property hinders the planning and management of single species tree plantations. In such situations, acquiring information about the available resources is challenging. This study aims to propose a method to locate and characterize tree plantations in these cases. Galicia (Northwest of Spain) is an area where property is extremely divided into small parcels. European chestnut (Castanea sativa) plantations are an important source of income there; however, it is often difficult to obtain information about them due to their small size and scattered distribution. Therefore, we selected a Galician region with a high presence of chestnut plantations as a case study area in order to locate and characterize small plantations using open-access data. First, we detected the location of chestnut plantations applying a supervised classification for a combination of: Sentinel-2 images and the open-access low-density Light Detection and Ranging (LiDAR) point clouds, obtained from the untapped open-access LiDAR Spanish national database. Three classification algorithms were used: Random Forest (RF), Support Vector Machine (SVM), and XGBoost. We later characterized the plots at the tree-level using the LiDAR point-cloud. We detected individual trees and obtained their height applying a local maxima algorithm to a point-cloud-derived Canopy Height Model (CHM). We also calculated the crown surface of each tree by applying a method based on two-dimensional (2D) tree shape reconstruction and canopy segmentation to a projection of the LiDAR point cloud. Chestnut plantations were detected with an overall accuracy of 81.5%. Individual trees were identified with a detection rate of 96%. The coefficient of determination R2 value for tree height estimation was 0.83, while for the crown surface calculation it was 0.74. The accuracy achieved with these open-access databases makes the proposed procedure suitable for acquiring knowledge about the location and state of chestnut plantations as well as for monitoring their evolution.
Radar-Based Non-Contact Continuous Identity Authentication
Authors: Islam Shekh Md Mahmudul , Borić-Lubecke Olga , Zheng Yao , Lubecke Victor M.
Published: July 2020

Non-contact vital signs monitoring using microwave Doppler radar has shown great promise in healthcare applications. Recently, this unobtrusive form of physiological sensing has also been gaining attention for its potential for continuous identity authentication, which can reduce the vulnerability of traditional one-pass validation authentication systems. Physiological Doppler radar is an attractive approach for continuous identity authentication as it requires neither contact nor line-of-sight and does not give rise to privacy concerns associated with video imaging. This paper presents a review of recent advances in radar-based identity authentication systems. It includes an evaluation of the applicability of different research efforts in authentication using respiratory patterns and heart-based dynamics. It also identifies aspects of future research required to address remaining challenges in applying unobtrusive respiration-based or heart-based identity authentication to practical systems. With the advancement of machine learning and artificial intelligence, radar-based continuous authentication can grow to serve a wide range of valuable functions in society.
Erratum: Bacour, C., et al. Simulating Multi-Directional Narrowband Reflectance of the Earth’s Surface Using ADAM (A Surface Reflectance Database for ESA’s Earth Observation Missions). Remote Sens. 2020, 12, 1679
Authors: Bacour Cédric , Bréon François-Marie , Gonzalez Louis , Price Ivan , Muller Jan-Peter , Straume Anne Grete
Published: July 2020

The authors wish to make the following corrections to this paper [...]
The Use of Factorial Design and Simplex Optimization to Improve Analytical Performance of In Situ Film Electrodes
Authors: Finšgar Matjaž , Jezernik Klara
Published: July 2020

This work presents a systematic approach to determining the significance of the individual factors affecting the analytical performance of in-situ film electrode (FE) for the determination of Zn(II), Cd(II), and Pb(II). Analytical parameters were considered simultaneously, where the lowest limit of quantification, the widest linear concentration range, and the highest sensitivity, accuracy, and precision of the method evidenced a better analytical method. Significance was evaluated by means of a fractional factorial (experimental) design using five factors, i.e., the mass concentrations of Bi(III), Sn(II), and Sb(III), to design the in situ FE, the accumulation potential, and the accumulation time. Next, a simplex optimization procedure was employed to determine the optimum conditions for these factors. Such optimization of the in situ FE showed significant improvement in analytical performance compared to the in situ FEs in the initial experiments and compared to pure in situ FEs (bismuth-film, tin-film, and antimony-film electrodes). Moreover, using the optimized in situ FE electrode, a possible interference effect was checked for different species and the applicability of the electrode was demonstrated for a real tap water sample.