bluuebunny/crossref_metadata_2025 huggingface dataset:
Penerapan Rebusan Air Daun Binahong Untuk Mempercepat Penyembuhan Luka Perineum Pada Ibu Post Partum
Authors: S Susanti , Yamin M , Nurcahyani Azizah
Published: December 2022

The World Health Organization (WHO), reports that as many 25% of MMR occur due to post partum problems.The direct factor causing the high AKI is post partum hemorrhage (45%). Post partum is the postpartum period which starts from the time the baby is born and the placenta of the baby is born so that the womb returns to what it was before pregnancy which lasts for six weeks.  Post partum hemorrhage due to the opening of blood vessels in the uterus during pregnancy due to the perineal wound can cause infection, Non-pharmacological management that can be given to accelerate the healing of perineal wounds is to use binahong leaf water boiled. To find out the application of giving binahong leaf water boiled to accelerate the healing of perineal wounds in post partum mothers. This study uses a literature review study that uses literature sources in the form of books, scientific articles, especially those that have been published. By using a search through the Google Scholar database from 2015-2020. Research result Shows that there is a significant effect on the application of giving binahong leaf water boiled to accelerate the healing of perineal wounds in post partum mothers. The application of boiled water from binahong leaves is more effective in accelerating the healing of perineal wounds in post partum mothers.
Analisis Aktivitas Fisik Ringan dan Berat Terhadap Kadar Hemoglobin
Authors: H Heriyanto , IS Indah Sari , A Aristoteles , B Bastian
Published: December 2022

Physical activity is all body movements caused by skeletal muscles with the need for energy expenditure. Physical activity is divided into 3 categories. Light, moderate and heavy physical activity. Heavy physical activity will increase metabolic activity, the acid produced in the form of hydrogen ions and lactic acid will increase a lot, this will cause a decrease in pH and intravascular hemolysis. The affinity between oxygen and hemoglobin will decrease when the blood pH is low. Hemoglobin is a tetrameric protein of erythrocytes that binds to non-protein molecules, namely iron porphyrin compounds called heme. This study aims to determine the effect of light and heavy physical activity on hemoglobin levels in students of the Faculty of Science and Technology IKest Muhammadiyah Palembang. This type of research is carried out in the form of experiments which are analyzed descriptively quantitatively and presented in the form of simple tables and diagrams. The sample was examined using a Hematology Analyzer with the Alfa Swelab Plus brand, the Pothomertic method. The results showed that the average hemoglobin level for light physical activity was 15.01g/dL and heavy physical activity was 15.2 g/dL with the One Way Anova test, the value = 0.689. The conclusion of the study showed that there was no effect between light and heavy physical activity on hemoglobin levels in students of the Faculty of Science and Technology IKest Muhammadiyah Palembang.
Study of Duality of Locally Convex Space
Authors: Amaresh Kumar  , Dr. Md. Mushtaque Khan
Published: December 2022

In this paper, we studied about the duality of locally convex space. The key to most of the results in topological vector space theory is to exploit duality - the relationship between on l.c.s. X and its dual X^*. The results of this section, particularly, show how this works. We will need to work with a variety of topologies on an l.c.s. X and it dual X^*. The results of this section, particularly, show how this works.
Teachers' Perceptions of Ibox Use for Integrated Science Lessons in Secondary Education Improvement Project Schools
Authors: Joshua Awuni  , Ezekiel Akotuko Ayimbila
Published: December 2022

The study sought to determine the level of use of iBox by integrated science teachers in the initial Secondary Education Improvement Project (SEIP) supported Senior High Schools in the Upper East Region of Ghana. This research tested teachers’ perceptions such as Computer Self Efficacy (CSE), Perceived Ease of Use (PEoU) and Perceived Usefulness (PU). The study employed a cross-sectional survey research design. Out of the 162 participants sampled, 155 questionnaires were fully completed and validated. Data analysis procedures included frequencies, percentages, crosstabs and chi-square tests. The results of the study indicated a statistically significant association between CSE and iBox utilisation and integration in science lessons. The results also showed a significant relationship between PEoU and iBox utilisation by teachers. Integrated Science teachers indicated a high PU but a statistical analysis revealed no significant association between PU and iBox use for science lessons. It was recommended among others that the GES and the SEIP to provide regular in-service training in information technology for integrated science teachers. It was suggested that future research be carried out on the differential perceptions of rural and urban SHS integrated science teachers of the benefits associated with iBox utilization.
NANOSPONGES : A New Approach for Drug Delivery System
Authors: G. J. Dhumal  , A. S. Kulkarni  , K. K. Bandal  , R. H. Dambe  , V. R. Thorat  , T. T. Patil  , K. K. Dhayagonde
Published: December 2022

An ideal drug delivery system will solubilize the drug and achieves proficient concentration of drug at the targeted site for a specific period of time in order to increase the bioavailability with minimal side effects. Topical drug delivery system challenged with many complications like poor permeability, skin irritation, allergic reactions etc. Nanosponges are one of the carriers which solve the difficulties of drug toxicity and poor bioavailability as they can entrap both lipophilic and hydrophilic drugs. Nanosponges are minute sized having diameter below 1µm with 3-dimensional network and they can easily penetrate through the skin. Nanosponges play vital role in targeting drug delivery in a controlled manner. They are highly porous in nature having ability to capsulate variety of drug moieties and offer controlled release at the targeted site. Nanosponges can be formulated as oral, parenteral, topical or inhalation dosage forms. Controlled release of the capsulated active agent at the targeted site and solubility improvement of poorly water-soluble drugs are key advantages of Nanosponges drug delivery systems. The present review discusses about Nanosponges, method of preparations, characterisation and applications particularly in the targeted drug delivery system.
Effect of Carbondioxide Pneumoperitoneum on Liver Enzymes Following Laproscopic Cholecystectomy In Rural Indian Population
Authors: Dr. Pusuluri Sai Vaibhav Kumar  , Dr. Purna Chandra Rao Paritala  , Dr. Sairam Peddireddy  , Dr. Kanna Chandra Varma  , Dr. Ashok Reddy
Published: December 2022

Introduction: Laparoscopic cholecystectomy, a minimal-access procedure for removing the gallbladder, has various benefits, including a significantly shorter hospital stay ,lower cost and a low patient comorbidity
Materials and Methods:- this was a prospective study. The study was conducted on 60 patients of cholecystectomy ,who underwent laparoscopic cholecystectomy under constant intraperitoneal pressure of 15mmhg in GEMS medical hospital, ragolu, Srikakulam, Andhra Pradesh. This study was designed to evaluate the complications of carbon dioxide pneumoperitoneum in laparoscopic cholecystectomy using the liver enzymes
Results:- Total bilirubin pre-operative was 0.72±0.13 mg/dl, increased 24 h after surgery to 1.11 ± 0.30 mg/dl by 138% . Aspartate aminotransferase (AST) pre-operative was 19.523 ± 16 U/l increased 24 h after surgery to 30.12± 18.35 U/l by 154 %. Alanine aminotransferase (ALT) was 22.58 ± 5.09 U/l increased 24 h after surgery to 38.62 ± 12.15U/l by 171%. Alkaline phosphatase pre-operative values were 103.8 ± 23.32 U/ and after 24 h was 147.8 ± 36.54 U/l by 142 %
Conclusion: laparoscopic operations produce a transient rise in serum liver enzymes ,our study too demonstrated bilirubin, AST, ALT, ALP elevated following laparoscopic cholecystectomy . when combined with data from earlier research These changes could be attributable to a decrease in portal venous flow caused by a pneumoperitoneum's high pressures.
Contribuição à História da Prevenção do Câncer no Instituto Nacional de Câncer
Authors: Carvalho Alexandre Octavio Ribeiro de , Almeida Liz Maria de , Franco Eduardo Barros
Published: December 2022

A partir da década de 1980, no contexto da redemocratização, e da adoção de políticas restritivas de gastos, o Instituto Nacional de  Câncer passou a coordenar a Política Nacional de Prevenção e Controle do Câncer, construindo uma agenda para prevenção do câncer, coordenada, cronologicamente, pelo Programa de Oncologia (Pro-Onco) (1988-1996), e seus sucessores, Coordenação Nacional de Tabagismo e Prevenção Primária (Contapp) (1996-1998), e a atual Coordenação de Prevenção e Vigilância (Conprev) (desde o final de 1998 até o presente).
Panorama da dança de salão: São Paulo (capital)
Authors: Lima Carmem Lúcia Leme de
Published: December 2022

A dança, atualmente, figura com evidência no mundo da arte e da cultura, e um dos estilos que retrata com maestria essa arte é a dança de salão, pois tem como finalidade preponderante dar prazer, conhecimento, expressar sentimentos e, claro, o desenvolvimento da saúde. Baseada em diversos saberes, a dança, enquanto movimento fundamentalmente humano, além do aspecto artístico, o desenvolvimento da criatividade, a afetividade, o aprendizado, a historicidade e a cultura, tem como figura central a pessoa que expressa, através da memória, o que viveu, o que pesquisou ou o que apreendeu. Nas suas lembranças, estão as marcas do tempo e dos grupos sociais aos quais pertencem, de acordo com cada história de vida. Esse estudo tem como objetivo traçar um panorama em diversos aspectos: histórico, inclusão, pessoas idosas, área acadêmica e de excelência ou profissional na dança de salão da capital de São Paulo, podendo servir de base para trabalhos futuros, realizada com o método de pesquisa bibliográfica e com a vivência da autora nas últimas décadas configurando o tema. Nesse contexto, essa pesquisa será efetiva na importância da valorização da dança de salão, na atualidade, na capital do Estado de São Paulo. No aspecto geral, o propósito deste estudo é trazer para o cenário acadêmico, além das experiências da modalidade de dança de salão na capital de São Paulo, a sua importância, desenvolvida pelas diversas instituições com diferentes características, e, também, considerar a dança de salão no aspecto técnico e artístico, trazendo mais conhecimento sobre essa área, despertando o interesse pelo tema. A metodologia buscou um levantamento técnico e acadêmico com referências teóricas sobre as teorias publicadas, reunindo textos que sustentem a ideia do artigo. Como resultado, conseguimos obter um arsenal de informações que nos trouxeram a realidade sobre a dança de salão na capital de São Paulo com um grande reconhecimento da modalidade, alcançando, na sua essência, suas características, trazendo, aos indivíduos, o sentimento de pertencimento através da integração com seu parceiro, com seus grupos, com a própria comunidade e, em consequência, com a sociedade, incorporando a integração, a intergeracionalidade, a inclusão e a questão ambiental.
Efficacy and safety of long-term enzyme replacement therapy with agalsidase alfa or agalsidase beta in adult patients with Fabry disease
Authors: Moiseev A. , Bulanov N. , Tao E. , Mershina E. , Sholomova V. , Moiseev S.
Published: December 2022

To evaluate the efficacy and safety of long-term enzyme replacement therapy (ERT) in the Russian population of patients with Fabry disease (FD).
Makna di Balik Iklan Traveloka PayLater “Baru Dari Traveloka PayLater!”
Authors: Ronaldo Siahaan Christo
Published: December 2022

Advertising is a work of art that can be interpreted. In interpreting advertising, there are meanings that appear depending on the perspective used. These meanings can then be used to determine whether an advertisement is good or bad. The good and bad of an advertisement will be seen from a certain point of view, such as the point of view of a critical consumer. From this point of view, an advertisement can be considered informative, relevant, and honest or it can be considered manipulative, irrelevant and deceptive. Advertisement “New from Traveloka PayLater!” has several meanings that can be interpreted from a certain point of view. Although the advertisement is sufficient to describe the advantages of the service, there are some dangers conveyed through the meanings that spread from the beginning to the end of the advertisement. Advertisement “New from Traveloka PayLater!” considered bad because it promotes consumptive behavior and hedonic culture, especially for the millennial generation.
Semiotika Iklan 3 Always On “Bebas Kini Semakin Nyata”
Authors: Irma Tri Anggraeni Irma
Published: December 2022

This paper discusses about how the 3 Always On try to advertise and tried to give a berakthriugh to the concept of freedom in people’s life. This concept oftenly being mistranslated. Although it is said to be free, the traditional form of stigma is actually able to break the concept of freedom itself. In the end, the term of a free and equal woman has never materialized. The 3 Always On adverstisement provides mild sarcastic of criticism to promote its produts, arguing on that it is unlimited. This explanation was being explained with the theory concept of Semiotic Theory by Roland Brathes.
Socio-Demographic Determinants of Genitourinary Prolapse
Authors: Zaman Riffat , Zaman Iffat , Rashid ABM Aminoor
Published: December 2022

Background: Genitourinary prolapse is a common gynecological problem, especially in developing country. Causes and risk factors for uterine prolapse are complex and deeply embedded in the cultural, economic and social conditions of women. Prevalence of depression is higher among women with advanced stages of prolapse. Hence this study aimed to assess the socio-demographic factors among patients with genitourinary prolapse.
Methods: A cross-sectional study was conducted from January to December, 2013 among 120 women with genital prolapse who attended at Gynae wards of Dhaka Medical College Hospital, Shaheed Suhrawardy Medical College Hospital, and Mitford Hospital in Dhaka. Women who were admitted with complaints of protruding mass per vagina and diagnosed as case of uterine prolapse were assessed and staged accordingly. A semi-structured questionnaire was used to obtain socio-demographic data and prolapse related data by face to face interview. After collection, data were compiled, summarized, and analyzed using SPSS software.
Results: In this study, out of 120 genitourinary prolapsed patients, 31.7% were 40-45 years age group, majority of 79.2 % were illiterate and almost two third had no monthly income. Among all respondents, 64.2 % had more than four children, maximum 96.7% had history of home delivery, and 91.7% history of heavy work during pregnancy or just after delivery.
Conclusion: Genitourinary prolapsed patients were illiterate, multipara, economic insolvency, heavy work during pregnancy and home delivery. A holistic management approach is recommended for women having prolapse.
JOPSOM 2021; 40(2):26-31
Actions aimed at repelling and deterring armed aggression of the Russian Federation in the context of the natural right to necessary defense
Authors: Kvasha O. , Andrusyak G.
Published: December 2022

Russia’s war against Ukraine necessitated the correction of domestic criminal legislation in this part as well. Law enforcement agencies throughout the country record the commission of a significant number of war crimes by the racist occupiers. The Armed Forces of Ukraine are putting up heroic resistance to the invaders, but ordinary citizens have to independently protect themselves and their loved ones from violence by the Russians.

The article substantiates the changes to Part 1 of Art. 36 of the Criminal Code of Ukraine “Necessary defense is recognized as actions taken with the purpose of protecting public interests and the interests of the state, as well as the legally protected rights and interests of the person being defended, or another person, from a socially dangerous encroachment by causing damage to the aggressor, necessary and sufficient in the given situation to immediately repel or stop the encroachment, if at the same time it was not allowed to exceed the limits of necessary defense”.

In accordance with the rules of legislative technique, the need to transfer the amendment provided for in Art. 43-1 of the Criminal Code of Ukraine, after as close as possible to it in terms of Art. 36 with assignment number 36-1.

In the context of the naturalness of a person’s right to necessary defense, such a specific manifestation (type) of it as “defense of the Motherland” should be defined as a willful and conscious act of a person, which is determined by the need to repel and contain the armed aggression of any country, other socially positive motives and goals, as well as natural (instinctive) human reactions.

Key words: aggression, natural law, protection of the Motherland, right, duty, armed aggression of the Russian Federation, necessary defense, national security, conditions of legality.
Constitutional and legal status and restriction of human and civil rights and freedoms under martial law in Ukraine
Authors: Krusian A. R.
Published: December 2022

The constitutional and legal status of a person and a citizen acquires peculiarities during the martial law, which was introduced on February 24, 2022 in connection with the military aggression of the Russian Federation against Ukraine and continues today.

The constitutional and legal status of a person and a citizen is a system of legal parameters enshrined in the Constitution and other normative legal acts that determine the real state of a person in society and the state at a specific stage of their socio-historical development, in specific political and legal conditions and modern realities. Structurally, this concept consists of such categorical means as: legal personality; civil status of the individual; principles of constitutional and legal status; rights, freedoms and responsibilities of man and citizen; legal guarantees as conditions for ensuring, realization and protection of human and civil rights and freedoms. During martial law, such elements of the constitutional and legal status of a person and a citizen as legal personality and civil status are relatively static (if possible, change them on certain subjective and objective grounds). Based on the theory and practice of modern Ukrainian constitutionalism under any circumstances, the principles of constitutional and legal status, enshrined in the Constitution of Ukraine (Articles 3, 21, 24, 25, 62, etc.) must remain unchanged.

The constitutional and legal status of a person and a citizen in martial law has certain features, which are manifested in the following: changing the legal basis of the status through emergency (temporary) and other regulations governing public relations directly in martial law; temporary restriction of rights and freedoms is established; the fulfillment of the constitutional duty to protect the Fatherland, independence and territorial integrity of Ukraine is actualized; means of exclusive protection of human and civil rights and freedoms are introduced; additional means of ensuring human and civil rights and freedoms are used. The specificity of the constitutional and legal status of a person and a citizen in martial law is the restriction of rights and freedoms, but provided that such a restriction is temporary and there is a constitutionally defined list of those rights and freedoms that cannot be restricted.

Key words: constitutional and legal status, human rights, Constitution, martial law, restriction of rights and freedoms.
Peculiarities of protection of children’s rights in conditions of military action and armed conflicts
Authors: Melnychuk O. F.
Published: December 2022

The article reveals the peculiarities of the protection of children’s rights due to hostilities and armed conflicts. The means that the state should use (both the victim and the aggressor) to protect the rights of children in the context of hostilities and armed conflicts are studied. Proposals are being made to improve national legislation and the collective security system. It is stated that the protection of children’s rights in the context of hostilities and armed conflicts has undergone transformations and has therefore acquired certain features distinct from those of peacetime. They boil down to the following features: children’s rights are protected by norms of both international and national law, but with the priority of international humanitarian law; the effectiveness of such protection depends on both parties to the armed conflict, including the good faith performance of the international legal obligations of the adversary; the system of collective security in modern conditions is inefficient and requires the development of new measures and tools for improvement; Consolidation of efforts and participation of the international community, creation of a coalition of states and various world organizations are important for the protection of children’s rights; The protection of children’s rights is facilitated by the social cohesion of civil society, which is acquiring new forms of activities related to helping children during hostilities and armed conflicts.

In order to effectively protect the rights of children in war zones and armed conflicts and children affected by them, national legislation needs to be further improved. To do this, it is necessary to develop a state program for the rehabilitation of children injured in hostilities and armed conflicts; determine the composition of such crimes as «recruitment of a child during an armed conflict», «use of a child during an armed conflict» and establish criminal penalties for them; define the concept of «a child who is in a zone of hostilities and armed conflict», etc. Ukraine currently lacks a consolidating public authority to ensure and protect the rights of children, including those affected by and affected by hostilities and armed conflicts.

Key words: children’s rights, protection of children’s rights, military action, armed conflict, Geneva conventions, international humanitarian law, collective security.
The specifics of law-making under martial law
Authors: Parkhomenko Natalia
Published: December 2022

The article describes the improvement of current legislation of Ukraine through introducing amendments and additions, along with enactment of a new law framework, regarding the need of organization of state machinery and legal order under martial law. The operation of state machinery, the interaction between the state and institutions of civil society, guaranteeing and protecting the rights, freedoms and legitimate interests of natural and legal person depend on the legislative compliance with the real social, political, economic and military challenges. In such circumstances, legal support of reform of political system of Ukraine under martial law is the primary objective for law-making actors in Ukraine.

The problem mentioned didn’t receive a thorough research and monitoring. In general, law-making process under martial law has proceed in accordance with constitutionally defined organizational and legal basis, meanwhile having some specifics. Among these are: the improvement of legislation system was carried out mainly not through enactment of primary laws, but through introducing amendments and additions to the legislation in all legal fields; the legal acts of military command and military administration that received relevant authority to make laws, were disseminated; there was a revitalization of legislative development in the military sphere; certain provisions of current legislation continued to operate on the temporary occupied territories; the restrictions on certain rights and freedoms of citizens were introduced in accordance with provisions of Constitution of Ukraine etc.

Due to the adoption of extraordinary legal acts, the legal arrangements for legal order and rule of law were created; public relations were stabilized.

Key words: law-making, authority, emergency, directive, order, martial law, command, legislation.
Factor Affecting Treatment Compliance in TB Patients
Authors: Lucya Vita , Arief Nabila Triefitriani
Published: December 2022

Aims: Pulmonary Tuberculosis is a disease caused by  Mycobacterium tuberculosis that invades the lungs. Indonesia ranks second as the country with the highest number of pulmonary TB in the world.  The treatment coverage of Pulmonary TB in Indonesia in 2022 is only 25% of the target (80%). One of the risk factors that cause treatment failure is medication adherence.
Objective:  This study aims to determine the factors that influence medication adherence in patients with pulmonary TB. 
Methods:  This study used a quantitative descriptive design with a cross-sectional study on 33 patients with pulmonary TB who became respondents by using total sampling. This study uses more than one instrument including Morisky Medication Adherence Scale-8, Generic Assessment of Side Effects, Multidimensional Scale of Perceived Social Support. The  data analysis that  were used in this study are Pearson Product Moment, Chi-square, Kruskal-wallis, and Simple Linear Regression.
Results:  The results of this study on factors related to medication adherence on TB patients showed that age, level of education, duration of treatment, drug side effects, and family support were related to medication adherence of pulmonary TB patients. alone.
Conclusion: This study showed that to achieve medication adherence requires the continuity of various factors and does not depend on one factor Future researchers  are expected to examine other factors that can affect medication adherence in patients with Pulmonary TB.
The Effect of Murottal Al-Quran Therapy on Anxiety Level of Chronic Kidney Disease Patients Undergoing Hemodialysis
Authors: Agustiyowati Tri Hapsari Retno , Endah Diana Ros , Putri Tri Antika Rizki Kusuma
Published: December 2022

Aims: Chronic kidney disease patients undergoing hemodialysis have several effects on dialysis, one of which is anxiety. According to several studies, anxiety can be overcome by murottal al-Qur'an music therapy. Listening to the Qur'an can have a calming effect on the body, because of the elements of autosuggestion meditation and relaxation. This relaxation will bring a positive perception that can stimulate the hypothalamus to secrete endorphins released by the pituitary gland and central nervous system to increase feelings of relaxation and distract from fear, anxiety, and tension.
Objective: to determine the effect of murottal al-Qur'an therapy on anxiety levels in chronic kidney disease patients undergoing hemodialysis.
Methods: This research is a quantitative study with a Quasy Experimental research design. One group pretest-posttest with a total sample of 34 respondents. The instrument used in this study is the Zung Self Anxiety Rating Scale (ZSAS) instrument, which is an assessment of anxiety in patients designed by William WK Zung which consists of 20 statements.
Results: Characteristics of respondents who took part in this study were aged 32-60 years, most of the respondents who took part in this study were aged 46-55 years as many as 17 respondents (50.0%), male (64.7%) , the length of time undergoing hemodialysis in the range of 7-44 months, most of the respondents underwent hemodialysis for a period of 6-12 months as many as 9 respondents (29.3%). The characteristic value of the ZSAS questionnaire, the largest value in the pre-test is at number 20 with a score of 126 while the largest value in the post-test is at number 5 with a score of 112. From the results of the Paired Simple T-test analysis, the p-value = 0.000, which means there is a significant relationship between murottal al-qur'an therapy and the anxiety level of chronic kidney disease patients undergoing hemodialysis.
Conclusion: the study showed that there was a significant effect on anxiety after being given murottal al-qur'an therapy from the Ar-Rahman surah. The murottal therapy expected to be implemented by nurses to reduce the anxiety level of chronic kidney disease patients undergoing hemodialysis.
Small intestinal submucosa-derived extracellular matrix as a heterotopic scaffold for cardiovascular applications
Authors: Palmosi Tiziana , Tolomeo Anna Maria , Cirillo Carmine , Sandrin Debora , Sciro Manuela , Negrisolo Susanna , Todesco Martina , Caicci Federico , Santoro Michele , Dal Lago Eleonora , Marchesan Massimo , Modesti Michele , Bagno Andrea , Romanato Filippo , Grumati Paolo , Fabozzo Assunta , Gerosa Gino
Published: December 2022

Structural cardiac lesions are often surgically repaired using prosthetic patches, which can be biological or synthetic. In the current clinical scenario, biological patches derived from the decellularization of a xenogeneic scaffold are gaining more interest as they maintain the natural architecture of the extracellular matrix (ECM) after the removal of the native cells and remnants. Once implanted in the host, these patches can induce tissue regeneration and repair, encouraging angiogenesis, migration, proliferation, and host cell differentiation. Lastly, decellularized xenogeneic patches undergo cell repopulation, thus reducing host immuno-mediated response against the graft and preventing device failure. Porcine small intestinal submucosa (pSIS) showed such properties in alternative clinical scenarios. Specifically, the US FDA approved its use in humans for urogenital procedures such as hernia repair, cystoplasties, ureteral reconstructions, stress incontinence, Peyronie’s disease, penile chordee, and even urethral reconstruction for hypospadias and strictures. In addition, it has also been successfully used for skeletal muscle tissue reconstruction in young patients. However, for cardiovascular applications, the results are controversial. In this study, we aimed to validate our decellularization protocol for SIS, which is based on the use of Tergitol 15 S 9, by comparing it to our previous and efficient method (Triton X 100), which is not more available in the market. For both treatments, we evaluated the preservation of the ECM ultrastructure, biomechanical features, biocompatibility, and final bioinductive capabilities. The overall analysis shows that the SIS tissue is macroscopically distinguishable into two regions, one smooth and one wrinkle, equivalent to the ultrastructure and biochemical and proteomic profile. Furthermore, Tergitol 15 S 9 treatment does not modify tissue biomechanics, resulting in comparable to the native one and confirming the superior preservation of the collagen fibers. In summary, the present study showed that the SIS decellularized with Tergitol 15 S 9 guarantees higher performances, compared to the Triton X 100 method, in all the explored fields and for both SIS regions: smooth and wrinkle.
Contribution of integrin adhesion to cytokinetic abscission and genomic integrity
Authors: Rani Bhavna , Gupta Deepesh K. , Johansson Staffan , Kamranvar Siamak A.
Published: December 2022

Recent research shows that integrin-mediated adhesion contributes to the regulation of cell division at two key steps: the formation of the mitotic spindle at the mitotic entry and the final cytokinetic abscission at the mitotic exit. Failure in either of these processes will have a direct impact on the other in each round of the cell cycle and on the genomic integrity. This review aims to present how integrin signals are involved at these cell cycle stages under normal conditions and some safety mechanisms that may counteract the generation of aneuploid cells in cases of defective integrin signals.
Establishment of a novel method to assess MEK1/2 inhibition in PBMCs for clinical drug development
Authors: Schüssele Lara M. , Koch-Heier Julia , Volk Julian , Löffler Markus W. , Hoffmann Katharina , Bruyns Regina M. , Planz Oliver
Published: December 2022

The Raf/MEK/ERK signaling pathway plays a key role in regulating cellular proliferation, differentiation, apoptosis, cytokine production, and immune responses. However, it is also involved in diseases such as cancer, and numerous viruses rely on an active Raf/MEK/ERK pathway for propagation. This pathway, and particularly MEK1/2, are therefore promising therapeutic targets. Assessment of target engagement is crucial to determine pharmacodynamics or the efficacy of a MEK1/2 inhibitor. In the field of infectious diseases, this is usually first determined in clinical trials with healthy volunteers. One method to detect MEK1/2 inhibitor target engagement is to assess the degree of ERK1/2 phosphorylation, as ERK1/2 is the only known substrate of MEK1/2. As healthy subjects, however, only feature a low baseline MEK1/2 activation and therefore low ERK1/2 phosphorylation in most tissues, assessing target engagement is challenging, and robust methods are urgently needed. We hence developed a method using PBMCs isolated from whole blood of healthy blood donors, followed by ex vivo treatment with the MEK1/2 inhibitor zapnometinib and stimulation with PMA to first inhibit and then induce MEK1/2 activation. As PMA cannot activate MEK1/2 upon MEK1/2 inhibition, MEK1/2 inhibition results in impaired MEK1/2 activation. In contrast, PMA stimulation without MEK1/2 inhibition results in high MEK1/2 activation. We demonstrated that, without MEK1/2 inhibitor treatment, MEK1/2 stimulation with PMA induces high MEK1/2 activation, which is clearly distinguishable from baseline MEK1/2 activation in human PBMCs. Furthermore, we showed that treatment with the MEK1/2 inhibitor zapnometinib maintains the MEK1/2 activation at approximately baseline level despite subsequent stimulation with PMA. As our protocol is easy to follow and preserves the cells in an in vivo-like condition throughout the whole handling process, this approach can be a major advance for the easy assessment of MEK1/2 inhibitor target engagement in healthy probands for clinical drug development.
Recent Progress on Carbon Quantum Dots Based Photocatalysis
Authors: Jung Hwapyung , Sapner Vijay S. , Adhikari Arindam , Sathe Bhaskar R. , Patel Rajkumar
Published: December 2022

Graphical Abstract
How to promote STEM competencies through design
Authors: Nichols Kim , Musofer Reshma , Haynes Michele
Published: December 2022

Introduction

This study explored the impact of a community of inquiry on Science, Design and Technology and Mathematics curriculum competencies of 159 Year six students as they carried out a design task.

Methods

A quasi-experimental design was employed with both qualitative and quantitative analyses. A community of inquiry approach was adopted by the teachers (n = 3) in the experimental group but not by teachers (n = 4) in the comparison group. Both groups participated in a learning sequence on electricity culminating in a design challenge in small collaborative groups.

Results

The results showed that the experimental group (n = 65) demonstrated significantly greater instances and a broader range of Science, Design and Technology and Mathematics competencies across the design task as well as significantly higher learning gains than their comparison group (n = 94) peers.

Discussion

The cognitive shifts towards higher competency development in the experimental group is stronger as a result of the reflection and reasoning required to engage in a community of inquiry.
The role of advanced glycation end products in fracture risk assessment in postmenopausal type 2 diabetic patients
Authors: Gao Liu , Liu Chang , Hu Pan , Wang Na , Bao Xiaoxue , Wang Bin , Wang Ke , Li Yukun , Xue Peng
Published: December 2022

Objective

The objective of this study was to analyze the quantitative association between advanced glycation end products (AGEs) and adjusted FRAX by rheumatoid arthritis (FRAX-RA) in postmenopausal type 2 diabetic (T2D) patients. The optimal cutoff value of AGEs was also explored, which was aimed at demonstrating the potential value of AGEs on evaluating osteoporotic fracture risk in postmenopausal T2D patients.

Methods

We conducted a cross-sectional study including 366 postmenopausal participants (180 T2D patients [DM group] and 186 non-T2D individuals [NDM group]). All the subjects in each group were divided into three subgroups according to BMD. Physical examination, dual-energy x-ray absorptiometry (DXA), and serum indicators (including serum AGEs, glycemic parameters, bone turnover markers and inflammation factors) were examined. The relationship between FRAX-RA, serum laboratory variables, and AGEs were explored. The optimal cutoff value of AGEs to predict the risk of osteoporotic fracture was also investigated.

Results

Adjusting the FRAX values with rheumatoid arthritis (RA) of T2D patients reached a significantly increased MOF-RA and an increasing trend of HF-RA. AGEs level was higher in the DM group compared to the NDMs, and was positively correlated with MOF-RA (r=0.682, P<0.001) and HF-RA (r=0.677, P<0.001). The receiver operating characteristic curve analysis revealed that the area under the curve was 0.804 (P<0.001), and the optimal AGEs cut-off value was 4.156mmol/L. Subgroup analysis for T2D patients revealed an increase in TGF-β, IL-6 and SCTX in the osteoporosis group, while a decreased PINP in the osteoporosis group compared to the other two subgroups. AGEs were positively associated with FBG, HbA1c, HOMA-IR, S-CTX, IL-6 and TGF-β in T2D patients, and negatively associated with PINP.

Conclusions

RA-adjusted FRAX is a relevant clinical tool in evaluating fracture risk of postmenopausal T2D patients. Our study analyzed the relationship between AGEs and FRAX-RA, and explored the threshold value of AGEs for predicting fracture risk in postmenopausal T2D patients. AGEs were also associated with serum bone turnover markers and inflammation factors, indicating that the increasing level of AGEs in postmenopausal T2D patients accelerated the expression of inflammatory factors, which led to bone metabolism disorders and a higher risk of osteoporotic fractures.
Chaotic honey badger algorithm for single and double photovoltaic cell/module
Authors: Almodfer Rolla , Mudhsh Mohammed , Alshathri Samah , Yousri Dalia , Abualigah Laith , Hassan Osama Farouk , Abd Elaziz Mohamed
Published: December 2022

PV cell/module/characteristic array accuracy is mainly influenced by their circuit elements, based on established circuit characteristics, under varied radiation and temperature operating conditions. As a result, this study provides a modified accessible Honey Badger algorithm (HBA) to identify the trustworthy parameters of diode models for various PV cells and modules. This approach relies on modifying the 2D chaotic Henon map settings to improve HBA’s searching ability. A series of experiments are done utilizing the RTC France cell and SLP080 solar module datasets for the single and double-diode models to validate the performance of the presented technique. It is also compared to other state-of-the-art methods. Furthermore, a variety of statistical and non-parametric tests are used. The findings reveal that the suggested method outperforms competing strategies regarding accuracy, consistency, and convergence rate. Moreover, the primary outcomes clarify the superiority of the proposed modified optimizer in determining accurate parameters that provide a high matching between the estimated and the measured datasets.
Tyre wear nanoparticles as test for a nano risk governance framework
Authors: van Broekhuizen Pieter , Säämänen Arto , Schuurbiers Daan , Isigonis Panagiotis , Jensen Keld A. , Kühnel Dana , Le Blansch Kees
Published: December 2022

The Horizon 2020 consortia NanoRigo, Gov4Nano and RiskGONE jointly developed a nano risk governance framework (NRGF), fit for use for the practice of nanomaterials’ development and use, understandable and executable for stakeholders involved in manufacturing, using or regulating nanomaterials, or confronted with incidental exposure to generated nanomaterials. The NRGF includes risk management models, tools and approaches relevant to nanomaterials, as well as nano-oriented LCA and grouping, and takes into account socio-economic aspects and risk-benefit assessment. Special attention was paid to operationalizing concern assessment within the framework of the risk governance approach, emphasizing the different nature of quantitative risk assessment and qualitative concern assessment. The usefulness and added value of the NRGF was tested by means of a case study on rubber tyres. This case study performed a “mock-up” risk and concern assessment of the use of manufactured nanomaterials in the rubber tyre tread and the environmental release of tyre wear particles (TWP) from this tread during car driving. With a focus on the release and hazards of nano-TWP, an approach using the NRGF methodology was defined in collaboration with a group of real-life stakeholders. Simultaneously a group of scientific experts assessed the usefulness of the available tools and methods for assessing this nano-TWP release and investigated related broader societal concerns. The exercise concludes that so far, an exclusive risk assessment of nano-TWP is a step too far, due to the lack of nano-specific health- and environmental exposure and hazard data. Moreover, it seems that public concern about nano-sized TWP emissions is still limited, contrary to emerging scientific concerns. The case study underlines the complexity of deriving robust recommendations for “real-life” cases. Many questions remain on how to weigh the available technical and social evidence in nano risk governance.
The biological transformation of ammonium and urea in a eutrophic estuarine system in Southern China
Authors: Tang Jin-Ming , Xu Min Nina , Lin Yuxuan , Chen Huangxin , Jin Haoquan , Han Li-Li , Zou Wenbin , Kao Shuh-Ji
Published: December 2022

Estuaries channel large amounts of anthropogenic nitrogen (N) from continents to the offshore where productivity is widely limited by N and phosphorus. Ammonium and urea, two reduced forms of anthropogenic N commonly observed, are the preferred substrates for various microorganisms (e.g., uptake by phytoplankton or bacteria and oxidation by nitrifier). Yet, it remains underexplored how they transform and their concentrations vary during transport in the estuary which may influence the offshore microbial community. We applied 15N isotope tracer incubation techniques to determine the two main bio-consumption processes, i.e., uptake and oxidation, of ammonium and urea, in the dark for the Jiulong River Estuary, a eutrophic estuary in southeastern China. Results showed that light penetration depth ranged from 0.8-3.3 m leaving 76% of estuary water bodies to stay in dark situations throughout a day. Ammonia oxidation, which favors dark conditions, dominates the estuarine regenerated-N cycle, showing the rank: ammonia oxidation > ammonium uptake >> urea uptake ≈ urea oxidation. By compiling the reported case studies, we found the relatively low ratio of urea oxidation to ammonia oxidation was accompanied by a relatively high ammonium concentration. Microorganisms’ high preference for ammonium over urea may lead to an inhibitory-like phenomenon. An analogous effect was evident by the increased urea uptake at downstream when ammonium was down to a few µM. The obstructed urea utilization resulted in 10 times longer lifetime for urea relative to ammonium (surface: 19 ± 9 days; bottom 12 ± 7 days). Such an inhibitory-like effect allows urea to be preserved in the estuary and allows urea to be transported farther offshore to stimulate microorganisms capable of utilizing urea, which may have significant impacts on offshore ecology.
Analysis of influencing factors on displacement sampling performance of deep-sea gas-tight sampler
Authors: Fang Yuanli , Chen Daohua , Deng Yinan , Huang Haocai
Published: December 2022

The collection and research of deep sea water has always been a hot and key topic in the field of marine research. Collecting seawater samples that can truly reflect the in-situ water composition information through a deep-sea sampler is an important technical means in the field of deep-sea exploration. The deep-sea water sampler designed in this subject samples deep-sea water by displacement sampling. According to the jet theory, the method of numerical calculation combined with experiment is adopted, the influence of indirect displacement, shape and diameter of sampling inlet, Coanda distance, sampling rate and other factors on the displacement effect is analyzed in this paper. The results of the study demonstrate that at low speed, multiple displacements can improve the displacement effect. In the high-speed sampling state, a single displacement method should be adopted. The design of making the sampling port as close as possible to the wall of the sampling cavity helps to strengthen the plane jet to improve the displacement efficiency. The displacement efficiency can be slightly improved by using the rectangular sampling inlet and outlet. As the extraction speed increases, the liquid mixing area becomes larger. Through discussion and comparison with similar research at home and abroad, the sampler has good performance and high sampling purity. The analysis of the effect of changes in sample temperature on the sampling displacement efficiency will be performed in the future. The research on the thermal insulation performance of the sampling channel will be put on the agenda.
Flexural behavior of reinforced concrete beams using waste marble powder towards application of sustainable concrete
Authors: Karalar Memduh , Özkılıç Yasin Onuralp , Aksoylu Ceyhun , Sabri Sabri Mohanad Muayad , Beskopylny Alexey N. , Stel’makh Sergey A. , Shcherban’ Evgenii M.
Published: December 2022

The performance of waste marble powder as a partial replacement for cement is examined with the aim to achieve more sustainable concrete. Pursuant to this goal, a total of 15 specimens were manufactured and then tested to examine the bending behavior. The effects of longitudinal reinforcement ratio and waste marble powder ratio were selected as variables. The experimental results showed that different proportions of tension reinforcement and waste marble powder had different crack and bending impacts on reinforced concrete beams. As the waste marble powder amount in the concrete mixture is increased from 0% to 40%, it was detected that the crack type changes from a shear crack from to a flexural crack as the amount of waste marble powder increases in the mixing ratio. The experimental findings revealed that the waste marble powder can be successfully used as 10% of the partial replacement of cement. Increasing the waste marble powder ratio by more than 10% can significantly decrease the capacity of the beams, especially when longitudinal reinforcement ratio is high. The influence of waste marble as partial replacement on the capacity decreases as the longitudinal reinforcement ratio decreases. Therefore, 10%–20% marble waste can be utilized as a replacement for cement when the longitudinal reinforcement ratio is close to the balanced ratio and more than 20% waste marble ratio should be avoided for any cases.