bluuebunny/crossref_metadata_2025 huggingface dataset:
SEPARATION OF ZIRCONIUM AND HAFNIUM WITH SIR METHOD’S (ADSORPTION AND DESORPTION ION EXCHANGE)
Authors: Biyantoro Dwi , Kundari Noor Anis , Aziz Nofriady
Published: December 2020

Precence of hafnium is  more  than 100 mg/kg making it impossible to use as cladding material in nuclear reactor, because of its high adsorption cross section thus inhibite the nuclear fission. The objective of this research is to separate Zr – Hf using SIR method that is by impregrnating extracts to  resin. Extractants used are TBP and D2EHPA, resin used is XAD – 7, and feed used is Zirconium Basic Sulphate (ZBS). In this research is done variation compotition SIR, optimation process adsorption and desoprtion SIR and determintionof equation model in adsorption SIR. The result of this research is composition SIR the most effective for separation Zr – Hf is 5 : 5 for dry method’s and 7 : 3 for wet metode’s, model of equation for Zr nearly Langmuir model, model of equation for Hf nearly Freundlich model and adsorption result effenctively is comparation feed : SIR yaitu 10 mL : 1,5 g (1 : 1,5) with separation factor 30.12 ; separation efficiency Zr 31.47 % and separation efficiency Hf  42.06%.
SINTESIS DAN KARAKTERISASI MATERIAL HIBRIDA Mg/AL-LDH TERIMOBILISASI ASAM PARA HIDROKSIBENZOAT
Authors: Hasnowo Lutfi Aditya
Published: December 2020

SINTESIS DAN KARAKTERISASI MATERIAL HIBRIDA Mg/AL-LDH TERIMOBILISASI ASAM PARA HIDROKSIBENZOAT. Telah dilakukan sintesis dan karakterisasi material hibrida Mg/Al-LDH terimobilisasi APHB. Mg/Al-LDH terimobilisasi APHB disintesis melalui sintesis tidak langsung dimana APHB diadsorpsikan pada material Mg/Al-LDH. Optimasi sintesis material hibrida dilakukan dengan mengamati pengaruh konsentrasi molar APHB dan pH sintesis. Padatan hibrida hasil sintesis dikarakterisasi dengan spektrometer XRD dan FTIR.Hasil penelitian menunjukkan bahwa sintesis Mg/Al-LDH terimobilisasi APHB optimum dilakukan pada pH 7. Berdasarkan model non linear isoterm Langmuir, diperoleh kapasitas Mg/Al-LDH untuk mengibolisasi APHB adalah 62,16 mg g-1, yang artinya per gram Mg/Al-LDH mengandung 62,16 mg g-1 APHB, dengan nilai konstanta Langmuir (Ka) sebesar 0,31 L mg-1. Hasil karakterisasi menggunakan XRD menunjukkan bahwa nilai parameter kisi a, kisi c dan basal spacing d003 hibrida Mg/Al-LDH terimobilisasi APHB masing-masing 3,06; 23,70 dan 7,09 Å, dimana APHB berada di permukaan luar lapisan hibrida. Hal ini juga didukung oleh hasil identifikasi melalui spektroskopi FTIR bahwa terdapat terdapat gugus-gugus fungsi spesifik asam para hidroksibenzoat di material hibrida tersebut
On Possibility of Perception of Modern Spanish Law Concepts of Legal Personality for Artificial Intelligence: a View from Russia
Authors: Shestak Viktor , Ilyicheva Zoya
Published: December 2020

Social life and technical innovations are always ahead of their legal regulation. This calls for a consistent reconsideration, renewal and improvement of currently existing legislation. Scientific discussions are now being held in Spain looking into the possibility of granting legal personality to robots. The increasing presence of artificial intelligence in our everyday life is rather objective. Nevertheless, it causes peoples inconvenience and anxiety. It is difficult for the legislator to define the level of his impact which he or she can actually have on the problems of legal regulation of the usage of using the artificial intelligence, including the issues concerning prevention of crimes that involve robots. The purpose of the following article is to study a number of concepts of legal personality of artificial intelligence in Spain. Some possibilities of proving a legal framework in the Spanish legislation for these concepts are considered. A number of probable problems caused by the vagueness of robots legal status are examined. The opportunity of perception of artificial intelligence as a subject of law in Spanish legislation is determined. The leading methods of this research were systematic and comparative approaches, methods of synthesis and analysis, which allowed to analyze, summarize and systematize the concepts of legal personality of artificial intelligence in Spain. As a result of studying the following issues, the authors came to the conclusion that there exists a crucial need to establish some limits of legal personality of robots. The public need for formation of a certain legal framework allowing to resolve ethical and legal conflicts with participation of artificial intelligence was discovered. The endeavor of society to harmonize the legislation in the field of robotics at the international level was revealed. The necessity of hindering the usage of robots for criminal purposes was proved.
Features of the Time Perspective in Adolescents with Health Disabilities
Authors: Trofimova Elena , Geranyushkina Galina , Malakhayeva Svetlana
Published: December 2020

The time prospective can be described as relation of a human being to his personal past, present and future. The time prospective determines the level of personal psychological health exercising influence upon self-efficiency of the human being, his readiness to overcome difficulties. A constructive solution of the identity crisis in adolescent age is not possible without understanding the dynamic changes and their connection with life events during various time periods. Solution of self-determination tasks, including those in terms of professional becoming presupposes the skills of setting prospective goals and of planning real steps in their implementation. The article examines the differences of perceiving time with the adolescents with health disabilities and the adolescents having normal health indicators. It analyses the inter-time ties, the order of priorities in time setups, the calendar periods of time prospective, the positive and negative motivational objects. The characteristics of adolescents with health disabilities depends on various indicators, and the determining indicator is the health defect which specifies the dependence of the individuals present and future activity.
Digital Medicine: Information and Technological Bases for Using Lexical Analysis in Formalization of Diagnosis Coding
Authors: Berseneva Evgeniya , Sukhodolov Alexander , Sedov Alexander , Mikhailov Dmitry , Kudrina Valentina , Andreeva Tatyana , Spasennikova Marina
Published: December 2020

The article brings forth the results of analysis of the existing practice of coding diagnoses, discusses the drawbacks of the existing model leading, in the long run, to impossibility of literate building of a monitoring system for morbidity rate and death rate. It substantiates the approaches, developed and proposed for implementation, to creating a lexical analysis module in terms of automated information systems of supporting the coding. The lexical analysis module under formation in terms of the automated coding system fits both for usage immediately at the moment of coding and post-factum and is topical (with certain technological improvement) for any version of the International Classification of Diseases. The efficiency of implementing the principles of creating and functioning of the system of the automated coding support in terms of the International Classification of Diseases with the lexical analysis module with support of transforming the clinical diagnosis into a formalized code is determined by the fact that usage of such a system increases the quality of forming the statistics of morbidity and death rates in the Russian Federation, which must allow to transfer the planning of medical aid and resource infrastructure to a new qualitative level.
Generating pseudo-random texts based on the frequency characteristics of texts in natural languages
Authors:   Novosibirsk State Technical University, Russian Federation, Kotov Yurii , Sanina Olga ,   Novosibirsk State Technical University, Russian Federation
Published: December 2020

The paper discusses generation of pseudo-random texts based on frequency characteristics of texts in natural languages. The follow frequency characteristics of texts and their values for the Russian and English languages are considered for generation: the distribution of unigrams and bigrams over frequency of occurrence in texts, the distribution of words over the length. Based on the considered frequency characteristics, an algorithm for generating pseudo-random texts is suggested. Texts generated according to the algorithm are studied in experiments of language recognition in texts.
Digital security: a new subject field in the focus of scientometrics
Authors:   Lomonosov Moscow State University, Russian Federation, Petrunin Yurii
Published: December 2020

The article explores the subject field of digital technology security. The article examines the specifics of this scientific field, its predecessors (information security, cybersecurity, etc.), the causes and consequences of its internal transformation, and the participation of various disciplines in this process. The method of research of problems of development of a new scientific direction in the article is scientometry, the source base is the Russian electronic library (NEB), the Russian science citation index (RSCI) and the scientometric Internet portal Google Academy. The article shows that if until recently a similar topic was associated with the key terms of technical, mathematical and managerial (automation, Cybernetics) Sciences, today it is supplemented with terms, methods and technologies of economic, legal and managerial Sciences (more than 60% of all publications). Not only the development of science and technology, but also changes in state policy in our country had a significant impact on this transformation and the subsequent rise in scientific activity. The study also showed that Russia has a serious reserve in this subject field, especially in technical and computer science.
Methods of resource modeling of organizational objects
Authors:   Joint-Stock Company Scientific Research Project Institute Civil Aviation ", Plotnikov Nikolai ,   AviaManager",   , Novosibirsk, Russian Federation
Published: December 2020

The development of resource methodology and soft computing in this paper is presented as a new direction of ontological design. The objects of air transport organizations are chosen as the applied area: a company, a social group, an individual. The existing understanding of re-sources is related to the concepts of consumption. The problem is that the category of re-sources is not identified and does not have a humanitarian study, primarily in logic, philosophy, and philology. The known classifications and types of resources are numerous, diverse, and mutually irreducible. A new understanding and logical elaboration of the "resource" category is proposed. The resource methodology offers its own bases and sources. The theoretical foundations are formed in the following content: principles, hypotheses, language, terms, conditions, and procedures. This content allows you to generate output of key definitions. Re-source design (modeling) is based on the concept of a resource model. Resource modeling can be defined as displaying object property parameters from a specified definition area to the area of indicator values. The proposed hypotheses of resource modeling consistency postulate that natural language tools: words, concepts, names, vocabulary, grammar, syntax, used in relations to describe objects, can be transformed into formal models that are suitable for subsequent calculations and automated management. Relations are displayed in the pseudo-physical logic of statements, which allows us to assign numbers to observable objects of non-numeric nature. Analogs of systemology are used to develop principles, conditions, and procedures for design and modeling. The basis of resource design is a formal resource model, on the basis of which elements are formed: complexes of contours, tuples of resources. Thus, the resource method al-lows us to assign numbers to the properties of objects in weak non-metric scales by shifting the assessment procedures to the area of strong metric scales, which establishes new combinations of applying qualitative and quantitative methods of research and development. A sample of practical resource design and modeling of objects in the subject area is presented on the example of the air transport complex.
Software description of the PID-controller algorithm in C# for use in a closed automatic control system
Authors:   University of Mediterranean Karpasia, Lefkosa, Kuzey Kıbrıs Türk Cumhuriyeti'nin, Piotrovskii Dmitrii , Podgorny Sergei ,   Kuban State Technological University, Krasnodar, Russian Federation, Kukolev Alexsander ,   Kuban State Technological University, Krasnodar, Russian Federation
Published: December 2020

Today, automatic control is increasingly associated with the capabilities of electronic compu-ting. If at the stage of the origin of the control theory, regulators were mainly mechanical de-vices with the simplest kinematics, now most regulators in production are electronic computing devices containing a Central processor, analog - to - digital converters, amplifiers, auxiliary switching equipment, peripheral devices as part of a human-machine interface. Responsibility for the correct and trouble-free operation and operation of such equipment is assigned to soft-ware that is a program code written in a specific programming language (Java, Python, C++, Pascal). Certain languages are adapted for specific purposes that determine their prevalence. Developed in 1998–2001 by Microsoft specialists, the object-oriented C# language has now gained considerable popularity due to its expressive syntax and ease of learning. The syntax of the language aims to level the complexity of C++, providing such impressive features as the use of lambda expressions, delegates, as well as providing direct access to memory. The number of applications written to date written in C# is incalculable due to the cross-platform nature of the .NET Core. For this reason, the authors attempted to consider the possibility of writing a software algorithm for a classical PID controller in the chain of the simplest first-order aperiodic link using this programming language.
Application of Deep Q-learning and double Deep Q-learning algorithms to the task of control an inverted pendulum
Authors:   Novosibirsk State Technical University, Russian Federation, Evseenko Alla , Romannikov Dmitrii ,   Novosibirsk State Technical University, Russian Federation
Published: December 2020

Today, such a branch of science as «artificial intelligence» is booming in the world. Systems built on the basis of artificial intelligence methods have the ability to perform functions that are traditionally considered the prerogative of man. Artificial intelligence has a wide range of research areas. One such area is machine learning. This article discusses the algorithms of one of the approaches of machine learning – reinforcement learning (RL), according to which a lot of research and development has been carried out over the past seven years. Development and research on this approach is mainly carried out to solve problems in Atari 2600 games or in other similar ones. In this article, reinforcement training will be applied to one of the dynamic objects – an inverted pendulum. As a model of this object, we consider a model of an inverted pendulum on a cart taken from the Gym library, which contains many models that are used to test and analyze reinforcement learning algorithms. The article describes the implementation and study of two algorithms from this approach, Deep Q-learning and Double Deep Q-learning. As a result, training, testing and training time graphs for each algorithm are presented, on the basis of which it is concluded that it is desirable to use the Double Deep Q-learning algorithm, because the training time is approximately 2 minutes and provides the best control for the model of an inverted pendulum on a cart.
Tools for text analysis based on the cryptanalysis of one-to-one substitution
Authors:   Novosibirsk State Technical University, Russian Federation, Makarskaya Darya , Kotov Yurii ,   Novosibirsk State Technical University, Russian Federation
Published: December 2020

The article considers text analysis tools based on a numerical estimate obtained using the cryptographic approach. It contains applying frequency cryptanalysis methods of one-to-one substitution to plaintext. In this case, these methods tend to methods for identifying letters of the text, and the task of cryptanalysis is to the task of identifying letters of text. For frequency cryptanalysis methods of one-to-one substitution, the identification error is determined as a statistic of a number of cryptanalysis errors for certain volumes of texts. The numerical score called the quality factor of a method can be transferred from methods to texts. Text analysis based on the quality factor of texts includes the selection of a cryptographic method with the help of which a quantitative assessment of the text is given, and the subsequent calculation of the quality factor of the text vector. In order to analyze texts based on cryptanalysis of a simple substitution the main stages of this analysis and necessary means are determined. These tools are implemented in the graphical user interface of MS Access and include 30 navigation tabs, on which 143 information and control elements are located. The article includes examples of implementation of some tools.
ALGORITHM OF COPROLOGICAL DI-AGNOSTICS OF INTESTINAL PROTO-SOZOSIS OF CALFS
Authors:   St. Petersburg State University of Veterinary Medicine, Gavrilova N. A. , Belova L. M. ,   St. Petersburg State University of Veterinary Medicine
Published: December 2020

The article presents the results of a exam-ination of calves under the age of 1 month, kept in one husbandry located on two farms in different districts of the Leningrad region. Diarrhea was found in 45% of calves at the farm in Osminovillage of, Luga district. In the Laboratory for the Study of Parasitic Diseases at the Department of Parasitology named after V. L. Yakimov at the St. Peters-burg State University of Veterinary Medi-cine, the scatological study algorithm con-sisted of making a direct smear, using the Darling’s method with an improved flotation fluid and staining fecal smears according to Romanovsky-Giemsa’s and Ziehl-Neelsen’s techniques. The staining of smears from fe-ces by the Romanovsky-Giemsa’s and Ziehl-Neelsen’s techniques made it possible to detect pathogens with a size of 3-5 microns, which are typical for Cryptosporidium sp. and Blastocystis sp. For the detection of blastocysts, the preferred diagnostic method is the Romanovsky-Giemsa staining of fecal smears, and for the detection of cryptospor-idia - Ziehl-Neelsen staining. Using this al-gorithm of scatological studies on a livestock farm located in the village of Porozhkino, the parasitic etiology of dysfunction of the gastrointestinal tract in calves was excluded. At the livestock farm in the village of Os-mino, a complex of scatological studies was carried out to identify the causative agents of cryptosporidiosis and blastocystosis, and recommendations were given on the imple-mentation of therapeutic and prophylactic measures on this farm. The diagnostic algo-rithm, which includes a step-by-step study of animal feces by the direct smear method, flotation method, staining of smears accord-ing to Romanovsky-Giemsa’s and Ziehl-Neelsen’s techniques allows us to identify a wide range of pathogens and establish not only mono, but also mixed infection
Satisfação dos usuários com a atenção à saúde bucal em Pauini, Amazonas, Brasil
Authors: Sobreira Folhadela Gustavo , Regis Aranha Lauramaris de Arruda , Silveira Pinto Adriana Beatriz , De Araújo Passos Shirley Maria , Monteiro Angela Xavier
Published: December 2020

OBJETIVO: Este estudo objetivou compreender a satisfação dos usuários com as Equipe de Saúde Bucal das Unidades Básicas de Saúde do município de Pauini, Amazonas. MATERIAL E MÉTODO: Este estudo transversal foi conduzido entre dezembro de 2019 e fevereiro de 2020 nas três unidades básicas de saúde da área urbana do município e teve como participantes os usuários dos serviços de saúde odontológicos.  Foram utilizados dois questionários: o primeiro para avaliar o acesso aos serviços de saúde e outro sobre a satisfação dos usuários com a atenção à saúde bucal.  Os resultados foram analisados de modo descritivo por meio de frequências absolutas e relativas. RESULTADOS: Participaram do estudo 265 indivíduos, e ao serem questionados como foi feito agendamento, 61,51% responderam que se deu por meio de visita à unidade de saúde, e com relação ao principal motivo para a última consulta, 47,17% dos usuários responderam que os principais motivos foram dor de dente e extração. Os percentuais mais elevados de satisfação foram encontrados no domínio estrutura física e o domínio com menores percentuais foram informação e apoio. CONCLUSÃO: os resultados evidenciaram necessidade de aperfeiçoar  a forma de acesso aos serviços de saúde público odontológicos e uma percepção positiva pelos usuários dos serviços de saúde bucal de Pauiní, AM, entretanto, os melhores resultados foram observados em relação à infraestrutura das unidades de saúde, e foi verificada necessidade de melhorar o processo de trabalho no que tange às informações e apoio aos usuários dos serviços de saúde bucal.
Analysis of Social Differentiation of Population in the Region
Authors:   Tambov State Technical University, Tambov, Russia, Bondarskaya T. A.
Published: December 2020

The choice and implementation of the appropriate strategy for the socio-economic development of the region are fundamental for meeting the social needs of citizens. At present, when determining the vector of economic development, municipalities take into account the trends in the development of society and the social problems arising in it, as well as their economic consequences. However, the radical transformation of the social sphere, which is so important for modern society, namely its social security, does not cease to segment society into opposite strata of different status. This topic is especially relevant for modern conditions, when the state guardianship is increasing in matters of conducting a modern and active social policy. The main task in this direction is the creation of such conditions for the population of the region, which could contribute to the comprehensive development of the social sphere. The analysis of indicators of the social sphere of the Tambov region is carried out, as well as problems and tasks for their solution are identified.
Prospects for the Development of the Digital Economy of Russia: the Current Status and Possibilities of a Digital Breakthrough
Authors:   Tambov State Technical University, Tambov, Russia, Bykovskaya E. V.
Published: December 2020

The functions and problems of the digital economy are considered, its assessment is carried out; the prospects for its development are analyzed. The conditions for the implementation and growth of the digital economy, its connection with related concepts have been studied. Both positive and negative aspects of digitalization processes in the economy are considered. The structure of the world economy, the market of digital technologies in Russia and abroad, innovative industrial enterprises implementing digital technologies are analyzed. The prospects for the development of digital technologies with the existing limiting factors are identified and substantiated. The approaches and factors influencing the current state and rebuilding of all domestic economic systems towards digitalization and the fourth industrial revolution have been determined. Within the framework of the study and its results, it was revealed that the state of all domestic economic systems, in particular industry, requires structural transformation to ensure a technological breakthrough, indicated in the new national projects of the Russian Federation, aimed at solving the abovementioned problems.
Organization of First-Year Students’ Engagement in Scientific Research Work (the Example of Future Teachers of Technology)
Authors:   P. P. Semenov-Tyan-Shanskiy Lipetsk State  Pedagogical University, Lipetsk, Russia, Tigrov V. P. , Gvozdeva A. G. ,   P. P. Semenov-Tyan-Shanskiy Lipetsk State  Pedagogical University, Lipetsk, Russia
Published: December 2020

The necessity of making first-year students of higher educational institutions engaged in research work is highlighted. The views of researchers on this issue are analyzed and general provisions are highlighted. The necessity of such work for the future technology teachers studying in the first year is explained. The final qualification work as a system-forming component of engagement of first-year students in research work is highlighted. The advantages of this approach and organizational aspects are given.
Peculiarities of Inter-Subject Relations in the Implementation of Military Professional Competences at Technical University of Telecommunications
Authors:   Bonch-Bruevich St. Petersburg State University of Telecommunications, St. Petersburg, Russia, Muzykantov A. N.
Published: December 2020

The article deals with interdisciplinary integration in its various manifestations, the process of its origin and formation, as well as the peculiarities of interdisciplinary relations in the implementation of military-professional competencies in a technical university as a whole and in the military training center of Bonch-Bruevich St. Petersburg State University telecommunications in particular.
Free Artist in the Strong Arms of Power (1928–2020)
Authors:   State Institute of Art Studies, Koljazin V.F.
Published: December 2020

In the summer of 1928, the famous Soviet Director, Creator of biomechanics and Director of the theater named after him, GOSTIM Vsevolod Emilevich Meyerhold (1874–1940) went to Paris to organize a foreign tour and suddenly received an order from the leadership of the cultural authorities to immediately return back. Tours are prohibited, and his theater faces liquidation. The Director is going through a severe moral crisis, a nervous breakdown forces him to go to the South of France for treatment. The subject of this article is the study of Parallels between the cultural policy of the Soviet party bureaucracy of the 1920s and the Russian authorities of the 2020s, which were particularly acute during the trial of the 7th Studio under the leadership of Kirill Serebrennikov. As before, so now the authorities exercise censorship and ideological supervision by means of administrative and financial regulation, regardless of the artist’s reputation and ethical issues. The article examines the obvious and secret reasons for the persecution of a prominent proletarian Director, the stages of his despair, emotions and resistance, as well as the picture of events on the other side of the fight in Moscow — in the corridors of the Narkompros. This is a period when the obvious opposition to Stalin to Meyerhold has not yet found clear outlines but has already affected indirectly, bringing the tragic denouement of the end of the 1930s — the closure of the theater and the execution of the Director. Most of the article is a montage of documentary archival materials published for the first time-transcripts of meetings and correspondence between Meyerhold and his wife Zinaida Reich with cultural authorities and friends in the theater (the so-called “frantic letters”). The drama of the artist and the triumph of a soulless bureaucracy speak for themselves in the documents of the era. The lesson of history, unfortunately, has not been learned, as the trial of the head of the Gogol Center Kirill Serebrennikov showed, fortunately, not deprived of the opportunity to work, but accused of embezzling a huge amount of money, allegedly irrationally used for productions in the youth 7th Studio.
Del sur al norte. El trayecto europeo del antillanismo caníbal
Authors: Erlendsdóttir Erla
Published: December 2020

En este artículo se estudia la presencia en las lenguas danesa e islandesa de la voz caníbal. Se trata de una palabra de origen antillano que ha penetrado en las lenguas europeas a través del español a partir del siglo XVI. Se pretende trazar el camino seguido por el vocablo seleccionado desde España —lengua vehicular de la mayoría de las voces amerindias prehispanas— hasta las lenguas receptoras: italiano, alemán, danés e islandés. Además de señalar la fecha y fuente de la primera documentación de la voz objeto de este estudio se examinarán los cambios formales y semánticos que ha sufrido.
https://doi.org/10.17398/2660-7301.40.25
FFPE tissue staining for Multiplexed Ion Beam Imaging (MIBI) analysis v1
Authors: Jiang Sizun , Rovira-Clave Xavier , Bosse Marc , C Bendall Sean , Angelo Michael , P Nolan Garry
Published: December 2020

Multiplexed ion beam imaging (MIBI) is a method that uses secondary ion mass spectrometry to image antibodies tagged with isotopically pure elemental metal reporters. It is capable of analyzing up to 50 targets simultaneously over a five-log dynamic range. MIBI was developed by Mike Angelo and Sean Bendall in the laboratory of Garry Nolan at Stanford. The current instrument is commercialized by IonPath Inc. The protocol below describes the staining of formalin-fixed, paraffin-embedded tissue sections. It was optimized in the Angelo and Bendall labs at Stanford, and further refined in the Nolan lab to its current form.
Affinity purification of ookinetes in Petri dishes v1
Authors: Recio Totoro Benito
Published: December 2020

The ookinetes, as they come from the ookinete culture, are suspended in a solution that also contains blood cells and other parasite stages. Often, it is necessary to purify the ookinetes from these other cell types. This is achieved exploiting biological, chemical, and physical properties of the ookinetes that differ from the other cell types present in the ookinete culture. This protocol exploits the adhesive and invading characteristics of ookinetes by allowing them to attach to a surface coated with Extracellular Matrix gel (ECM gel). The rest of the cells present in the ookinete culture do not have adhesion properties and can be simply washed away. ECM gel is composed of approximately 1800 proteins, the most abundant are type IV collagen, laminin, entactin, and other proteoglycans. The Plasmodium ookinete has several surface proteins that bind to the ECM gel components, such as the P25, and P21/28 protein families that bind to laminin and collagen, and the CTRP, SOAP, and WARP surface or secreted proteins that bind to laminin, and other glycosaminoglycans. The purification in Petri dishes allows the obtention of a higher amount of ookinetes with the expense of being less pure than the purifications on coverslips. The ookinetes can be re-suspended by the use of dispase II, a protease that cleaves type IV collagen and repurposed for experiments that require them in suspension or to perform organelle and macromolecule extracts.
Cell DIVE™ Platform | Antibody Staining & Imaging v1
Authors: McDonough Liz , Chadwick Chrystal , Ginty Fiona , Surrette Christine , Sood Anup
Published: December 2020

This document outlines the workflow for antibody staining, dye inactivation, and imaging as per the Cell DIVE™ technology.
Quantification of plant hormones by standard addition method v1
Authors: Hashiguchi Takuyu , Hashiguchi Masatsugu , Tanaka Hidenori , Fukushima Koki , Gondo Takahiro , Akashi Ryo
Published: December 2020

Phytohormones are essential signaling molecules in multiple plant processes, including growth, development, and stress response. Simultaneous quantification of multiple plant hormones is required for understanding plant physiology because hormones typically change in tandem in response to external stimuli. Sensitive and quantitative analysis using liquid chromatography-linked mass spectrometry (LC-MS/MS) adopts stable isotope-labeled compounds in previously published works. However, the method for quantifying phytohormones when isotopically labeled chemicals are not available, remains to be established. This protocol can be used for measuring plant hormone content by standard addition method. Standard addition method has been used for quantification of various compounds such as drugs and pesticides especially when it is difficult to obtain stable isotope-labeled compound. Plant hormones are extracted from plant material such as Lotus japonicus with organic solvent and partially purified with reversed-phase solid-phase extraction (SPE) and ready for LC-MS/MS analysis. Calibration curve is constructed by spiking pure standards to matrix. The method allows us to quantify plant hormones in plant tissue of interest by correcting matrix effects.
Plasmid Sequence Analysis from Long Reads v3
Authors: A Eccles David
Published: December 2020

This protocol demonstrates how to assemble reads from plasmid DNA, and generate a circularised and non-repetitive consensus sequence At the moment, this protocol uses Canu to de-novo assemble high-quality single-cut reads. Input(s): demultiplexed fastq files (see protocol Demultiplexing Nanopore reads with LAST). I've noticed that the default demultiplexing carried out by Guppy (at least up to v4.2.2, as used in the first version of this protocol) has issues with chimeric reads, which can affect assembly. Output(s): Consensus sequence per barcode as a fasta file
Identities in Seamus Heaney’s Translation of Beowulf
Authors: Nakova Katileva Eleonora
Published: December 2020

The present article sets out to prove the hypothesis that the Modern English translation of Beowulf by Seamus Heaney reflects his Irish political and cultural roots. His interpretation aroused the interest of critics by its use of Hiberno-English and dealing with linguistic structural tasks in a different way for the first time. By considering specific examples from the original and the translated version of the poem, the present article analyses the linguistic choices made by Heaney in his translation of the Old English version of Beowulf taking into account its critical reception and the author’s personal opinions and experiences. It sets out to establish the roots of this translation in Heaney’s upbringing in rural Ireland by observing specific memories from his own childhood, family members, politics and surroundings. The article also compares this translation to previous ones to provide the reasons for the uniqueness of Heaney’s rendering and establish its importance in today’s literary scene.
Charles V’s ‘Encomium Mori’ as Reported By Ambassador Elyot
Authors: Olivares Merino Eugenio M.
Published: December 2020

William Roper is the author of the first and most influential biography of Sir Thomas More, his father-in-law, finished in 1557. As stated in this source, shortly after More’s execution for high treason at the Tower of London (1535), the Emperor Charles V met Thomas Elyot then serving as ambassador at the imperial court. The content of this meeting was later on disclosed by Elyot himself to some members of More’s closest circle, among them Roper himself, whose testimony has remained the ultimate source of the episode. As soon as Charles had come to know about More’s execution, he communicated the news to Elyot and shared with him his admiration for the ex-Chancellor. Several scholars, however, have questioned the reliability of Roper’s memory in the light of historical evidence for Elyot’s whereabouts at the time of More’s death. This paper revises the main stances in the discussion of this episode, and brings into consideration other issues that might cast some light, not only on the details of this story, but also on the relationship between these two Thomases (More and Elyot) and Charles, the most powerful ruler in Europe at the time.
Flight to Canada And Kindred: Similarities and Discrepancies in Two Neo-Slave Narratives Translated into Spanish
Authors: Sanz Jiménez Miguel
Published: December 2020

The aim of this paper is to study the Spanish translations of Ishmael Reed’s Flight to Canada and Octavia E. Butler’s Kindred, two neo-slave narratives that were published in the 1970s. It examines how Black English, the lexicon of slavery, and proper nouns have been recreated in the Spanish target texts. The linguistic variety spoken by the secondary characters in Flight to Canada and by the slaves in Kindred makes readers aware of the language of the dispossessed Other. Butler’s and Reed’s novels were published simultaneously in Spain in 2018 and translated by Amelia Pérez de Villar and Inga Pellisa, respectively. This paper observes how translators’ choices play a key role in the portrayal of alterity in literary texts.
Transforming Human Rights through Decolonial Lens
Authors: Gómez Sánchez Davinia
Published: December 2020

This article problematizes the Human Rights conceptualization embodied in the International Human Rights Law corpus. It considers human rights as a Western construct rooted in a particular historical context, located in a specific ideological background and grounded in a concrete socio-cognitive system. Thus, in disregard of features of non-dominant cultures, the mainstream human rights grammar became a discourse of empire. Building on TWAIL and decolonial theory, this article challenges that hegemonic human rights discourse while providing a justification for incorporating other conceptualizations of rights through an inter-epistemic conversation with alternative world-views.
Graph Partitioning Based on the Combined Approach
Authors:   Southern Federal University, Rostov-on-don, 344006, Russian Federation, Kureichik V. V. , Kureichik Vl. Vl. ,   Gazprom subterranean repair Urengoy LLC, Saint-Petersburg, 190000, Russian Federation
Published: December 2020

The article considers one of the most important combinatorial optimization problems — the problem of graph partitioning. It belongs to the class of NP-complex optimization problems. The article presents the partitioning problem statement. Due to the complexity of this task, the article proposes a new search strategy based on a combined approach. The combined approach is to divide the decision-making process into two levels. At the first level, the bee optimization method is used to quickly obtain subdomains with a high value of the objective function, and at the second level, an evolutionary algorithm is used to improve obtained solutions. To implement this approach, the authors developed a combined algorithm that can obtain sets of quasi-optimal solutions in polynomial time and avoid looping in local regions at the same time. A software module is developed and algorithms for partitioning graphs into parts are implemented. A computational experiment has been carried out when dividing into 8 parts of test circuits (benchmarks) by IBM. An analysis of experimental studies showed that the developed combined algorithm is on average 5 % higher than the partition results obtained by well-known hMetis, PGAComplex algorithms with comparable solution time, which indicates the effectiveness of the proposed approach. The time complexity of the developed combined algorithm is approximately O (n2).
Method for Improving the Efficiency of IT Project Management Using a Genetic Algorithm
Authors:   nter-State Educational Institution of Higher Education Belarusian-Russian University, Mogilev, Republic Belarus, Vaynilovich Yu. V.
Published: December 2020

The article is devoted to solving the current problem of improving the efficiency of IT project management processes. When managing IT projects, managers are faced with the problem of formation teams and distributing tasks among project participants in the face of the need to minimize costs and completion dates of an IT project. The lack of necessary methods and software doesn't allow the IT project Manager to adequately assess competences and skills of participants, their personal qualities, which leads to a decrease in the effectiveness of project management. The article proposes a method of improving the efficiency of IT project management, which differs by using a genetic algorithm to form project commands and assign team participants to project tasks. The efficiency criterion is the complexity and duration of the project and individual tasks using the COCOMO II method. When forming project teams, takes into account the level of technologies proficiency, experience with technologies, the coherence of the project team members, and the experience of similar developments of project participants. The level of technologies proficiency affects the level of labor input multiplier, experience with technologies — at the level of the multiplier, the coherence of the project team members — on the level of scale factor, the experience of similar development — on the level of the scale factors of the COCOMO II methodology. Taking into account the personal and psychological qualities of project participants reduces the risk of interpersonal conflicts within the team, which also reduces the duration of projects and the labor input of solving tasks. Research of personal and psychological qualities is carried out on the basis of automated tests. The test suite includes Rosenzweig, Belbin, Myers-Briggs, Thomas and Honey-Mumford tests. The developed method is implemented in a software complex for multilevel IT project management. Testing of the method and software complex was carried out within the framework of the students' learning practice of the specialty "Software engineering" of the Belarusian-Russian University. The use of the proposed method allowed to reduce the labor input of solving the tasks of training projects by 19.2 %, to reduce the project realization term by 10 %.
Urban Connective Action: The Case of Events Hosted in Public Space
Authors: McGillivray David , Guillard Severin , Reid Emma
Published: December 2020

In the past decade, significant transformations have influenced the governance of urban public spaces. There has also been a growth in new public spheres associated with digital media networks, informing and influencing the production and regulation of urban space. In this article, we explore the role of digital and social media as a form of connective action supporting public campaigns about the privatisation and erosion of public space in the Scottish city of Edinburgh. We draw on analysis of Twitter data, interviews and observations of offline events to illustrate how a broad coalition of actors utilise online and offline tactics to contest the takeover of public space, confirming that that the virtual and the physical are not parallel realms but continuously intersecting social realities. Finally, we reflect on the extent to which digital media-enabled connective action can influence the orientation of urban controversies debates and lead to material change in the way urban public space is managed and regulated.
RESEARCH OF WAYS TO INCREASE CORROSION RESISTANCE IN REINFORCED CONCRETE CONSTRUCTIONS. PART 2
Authors: KONDRATYEVA Nadezhda V. , ALFIMENKOVA Alexandra Yu.
Published: December 2020

The article presents the results of the fi nal series of tests on the study of ways to increase the corrosion resistance of reinforced concrete structures with primary protection in the form of an addition of acetone-formaldehyde resin ACF-75 and with secondary protection with a two-component resin Binder EP 11 Tikso. An analysis of the change in the adhesion strength of secondary protection elements with a concrete surface after exposure to sulfuric and nitric acid, as well as the eff ect of the presence of a preliminary primer on a concrete surface on the intensity of adhesion change is presented. Conclusions are drawn about the possibility of using a two-component resin Binder EP 11 Tikso as a secondary protection against corrosion destruction of concrete in the process of reinforcing reinforced concrete structures operating in environments containing sulfuric and nitric acids.
TESTING OF THE JOINTS ON POP RIVETS WITH BULGE
Authors: SHIROKOV Viacheslav S. , SOLOVEV Alexey V. , IGOLKIN Sergey A.
Published: December 2020

The experience of testing of joints on pop rivets, which formed the basis of design standards, is considered. Experience of testing joints on pop rivets of sheets having bulge, carried out at the Metal and Wood Structures Chair of the Samara State Technical University is given. According to the test results, the maximum load on the joints was obtained, which turned out to be 20% higher than the value determined by the design standards for similar joints, but without bulge
Humanitarian resistance in the context of digitalisation of education
Authors: Murzina I. Ya.
Published: December 2020

Introduction.In the context of a pandemic, significant transformations in education have occurred: the urgent shift to new forms of learning and digitalisation of the learning process have become a challenge to the traditional understanding of education as a public good, based on humanistic grounds. In the media and social networks, there was a public debate over the prospects of the education for several months. The disparity in opinions and not always accurate determination of the positions of the authors of numerous publications highlight the need for analysing the situation in modern education from the standpoint of axiology and cultural studies.

Aim.The present publication isaimedto consider the current changes in education in the conditions of digitalisation and to identify the potential of humanistic pedagogy.

Methodology and research methods.The study is based on an interdisciplinary methodology, which integrates the achievements of the humanities and social sciences. The method of cluster analysis, content analysis in the mass media and social media, supplemented by group identification analysis, were employed.

Research results.According to the findings of the present study, it was concluded that the contemporary processes in Russian education can be described from the point of view of trauma theory. The author introduces the concept of “humanitarian resistance”, which is referred to the forms of symbolic protest, based on the values of humanism, national and cultural traditions, and non-violence. Furthermore, the author proposes a primary classification of the forms of humanitarian resistance in Russian education, including worldview, axiological, socio-psychological and pedagogical aspects. In the current article, the essence of each aspect is disclosed on specific examples.

Scientific noveltyof the conducted research lies in the theoretical justification of the analysis of current educational practices from a philosophical and cultural point of view. The introduction of new categories into scientific circulation allows for an adequate description of the current processes in education. The article is debatable, involving the discussion on the problems of education as a public good and the teacher as a bearer of the humanistic tradition in terms of digitalisation.

Practical significanceof the research consists in the possibility of using its results to determine the prospects of educational policy in the digital society, as well as to develop the strategies and to select teacher training routes in new conditions.
Germańskie zapożyczenia morfemowe w bośniackiej leksyce sportowej
Authors: Bońkowski Robert
Published: December 2020

W artykule poddano badaniu adaptacje germańskich zapożyczeń morfemowych w języku bośniackim, ze szczególnym uwzględnieniem słownictwa sportowego. Przeprowadzona analiza ukazała specyfikę jego asymilacji w języku bośniackim oraz ukazała zasób formantów wykorzystywanych w procesie tejże adaptacji. Badanie potwierdziło również ustalenia bośniackich standardologów dotyczące otwartości języka bośniackiego na zapożyczenia językowe oraz, a może przede wszystkim, ujawniło współwystępowanie dubletów leksykalnych. W badanym materiale wariantywność ta polega na użyciu różnych morfemów słowotwórczych (sufiksów), które są dodawane do tego samego morfemu rdzennego, w wyniku czego dany desygnat może posiadać dwie postaci.