bluuebunny/crossref_metadata_2025 huggingface dataset:
Importance of the PD-1/PD-L1 Axis for Malignant Transformation and Risk Assessment of Oral Leukoplakia
Authors: Ries Jutta , Agaimy Abbas , Wehrhan Falk , Baran Christoph , Bolze Stella , Danzer Eva , Frey Silke , Jantsch Jonathan , Möst Tobias , Büttner-Herold Maike , Wickenhauser Claudia , Kesting Marco , Weber Manuel
Published: February 2021

Background: The programmed cell death ligand 1/programmed cell death receptor 1 (PD-L1/PD-1) Immune Checkpoint is an important modulator of the immune response. Overexpression of the receptor and its ligands is involved in immunosuppression and the failure of an immune response against tumor cells. PD-1/PD-L1 overexpression in oral squamous cell carcinoma (OSCC) compared to healthy oral mucosa (NOM) has already been demonstrated. However, little is known about its expression in oral precancerous lesions like oral leukoplakia (OLP). The aim of the study was to investigate whether an increased expression of PD-1/PD-L1 already exists in OLP and whether it is associated with malignant transformation. Material and Methods: PD-1 and PD-L1 expression was immunohistologically analyzed separately in the epithelium (E) and the subepithelium (S) of OLP that had undergone malignant transformation within 5 years (T-OLP), in OLP without malignant transformation (N-OLP), in corresponding OSCC and in NOM. Additionally, RT-qPCR analysis for PD-L1 expression was done in the entire tissues. Additionally, the association between overexpression and malignant transformation, dysplasia and inflammation were examined. Results: Compared to N-OLP, there were increased levels of PD-1 protein in the epithelial and subepithelial layers of T-OLP (pE = 0.001; pS = 0.005). There was no significant difference in PD-L1 mRNA expression between T-OLP and N-OLP (p = 0.128), but the fold-change increase between these groups was significant (Relative Quantification (RQ) = 3.1). In contrast to N-OLP, the PD-L1 protein levels were significantly increased in the epithelial layers of T-OLP (p = 0.007), but not in its subepithelial layers (p = 0.25). Importantly, increased PD-L1 levels were significantly associated to malignant transformation within 5 years. Conclusion: Increased levels of PD-1 and PD-L1 are related to malignant transformation in OLP and may represent a promising prognostic indicator to determine the risk of malignant progression of OLP. Increased PD-L1 levels might establish an immunosuppressive microenvironment, which could favor immune escape and thereby contribute to malignant transformation. Hence, checkpoint inhibitors could counteract tumor development in OLP and may serve as efficient therapeutic strategy in patients with high-risk precancerous lesions.
Dielectrophoretic Characterization of Tenogenically Differentiating Mesenchymal Stem Cells
Authors: Giduthuri Anthony T. , Theodossiou Sophia K. , Schiele Nathan R. , Srivastava Soumya K.
Published: February 2021

Tendons are collagenous musculoskeletal tissues that connect muscles to bones and transfer the forces necessary for movement. Tendons are susceptible to injury and heal poorly, with long-term loss of function. Mesenchymal stem cell (MSC)-based therapies are a promising approach for treating tendon injuries but are challenged by the difficulties of controlling stem cell fate and of generating homogenous populations of stem cells optimized for tenogenesis (differentiation toward tendon). To address this issue, we aim to explore methods that can be used to identify and ultimately separate tenogenically differentiated MSCs from non-tenogenically differentiated MSCs. In this study, baseline and tenogenically differentiating murine MSCs were characterized for dielectric properties (conductivity and permittivity) of their outer membrane and cytoplasm using a dielectrophoretic (DEP) crossover technique. Experimental results showed that unique dielectric properties distinguished tenogenically differentiating MSCs from controls after three days of tenogenic induction. A single shell model was used to quantify the dielectric properties and determine membrane and cytoplasm conductivity and permittivity. Together, cell responses at the crossover frequency, cell morphology, and shell models showed that changes potentially indicative of early tenogenesis could be detected in the dielectric properties of MSCs as early as three days into differentiation. Differences in dielectric properties with tenogenesis indicate that the DEP-based label-free separation of tenogenically differentiating cells is possible and avoids the complications of current label-dependent flow cytometry-based separation techniques. Overall, this work illustrates the potential of DEP to generate homogeneous populations of differentiated stem cells for applications in tissue engineering and regenerative medicine.
Shared Developmental Trajectories for Fractional Reasoning and Fine Motor Ability in 4 and 5 Year Olds
Authors: Clark Lindsey , Shelley-Tremblay John , Cwikla Julie
Published: February 2021

We investigated preschool-aged children’s understanding of early fractional tasks and how that performance correlates with fine motor skills and use of gestures while counting. Participants were 33 preschoolers aged 4 to 5 in two Southeastern public elementary schools. Children were tested individually in an interview-like setting. Mathematics tasks were presented in a paper and pencil format and the Grooved Pegboard test assessed fine motor skills. Finally, utilization of gestures was evaluated by taking a behavioral rating of the child’s hand morphology, accuracy of gestures, and synchrony of gestures and spoken word while performing a counting task. Results indicate that performance on fractional reasoning tasks significantly predicts both fine motor ability and accuracy of gestures.
Effects of the Earthquake Nonstationary Characteristics on the Structural Dynamic Response: Base on the BP Neural Networks Modified by the Genetic Algorithm
Authors: Zhang Yunlong , Du Dongsheng , Shi Sheng , Li Weiwei , Wang Shuguang
Published: February 2021

The intensity non-stationarity is one of the basic characteristics of ground motions, the influences of which on the dynamic responses of structures is a pressing issue in the field of earthquake engineering. The BP neural network modified by the genetic algorithm was adopted in this research to investigate the influence of intensity nonstationary inputs on the structural dynamic responses from a new perspective. Firstly, many training data were generated from the prediction formula of dynamic response. The BP neural network was then pre-trained by sparsely selected data to optimize the initial weights and biases. Finally, the BP neural network was trained by all data, and the mean square error of predicted responses compared with the target response were less than 10−5. The calculation formula of sensitivity was also derived here to quantify the influence of the input change on the output. The presented method combines the advantages of neural networks in nonlinear multi-variable fitting and provides a new perspective for the study of earthquake nonstationary characteristics and their influence on the structural dynamic responses.
Gold Nanoframe Array Electrode for Straightforward Detection of Hydrogen Peroxide
Authors: Purwidyantri Agnes , Tian Ya-Chung , Saputra Gardin Muhammad Andika , Prabowo Briliant Adhi , Liu Hui-Ling , Yang Chia-Ming , Lai Chao-Sung
Published: February 2021

The nanostructuring of a sensing membrane is performed through colloidal nanosphere lithography (NSL) techniques with a tiny polystyrene nanobead template 100 nm in size. The solvent ratio adjustment has been proven to be effective in assisting the monolayer deposition of small templating particles with minimal defects. Two distinct structures, namely, a billowy gold nanostructure (BGN) where the nanobead template is left unetched and a gold nanoframe array (GNA) with a regular ring-like structure after template removal, are used for the extended-gate field-effect transistor (EGFET) electrodes. The GNA structure generates an electroactive surface area significantly (~20%) larger than its geometrical area as well as a greater surface roughness than the BGN. When integrated with the portable constant voltage–constant current (CVCC) FET circuitry for pH screening to determine the optimized measurement conditions for H2O2 sensing, the GNA sensing membrane also shows more improved Nernstian sensitivity at ~50 mV/pH than the BGN electrode. The more optimized sensitivity is then proven using the GNA in the detection of H2O2, the most common representative reactive oxygen species (ROS) involved in the environment, food, and neurodegenerative diseases, such as Parkinson´s and Alzheimer´s diseases. The GNA electrode has a sensitivity of 70.42 mV/log µM [H2O2] and a limit of detection (LoD) of 1.183 µM H2O2. The integrated ion sensing system employing unique, highly ordered gold array gate electrodes and a portable CVCC circuit system has shown a stable real-time output voltage signal, representing an alternative to bulky conventional FET devices for potential on-site H2O2 detection.
Electrorheological Effect of Gold Nanoparticles Coated with Fluorescent Mesogenic Groups Dispersed in Nematic Liquid Crystal
Authors: Kaneko Kosuke , Yamashita Kosuke , Fujioka Daiki , Kaneko Kimiyoshi , Fuchigami Kiyomi , Hashishin Takeshi , Hanasaki Tomonori
Published: February 2021

The electrorheological (ER) properties of composite materials consisting of a nematic liquid crystal (LC) and gold nanoparticles (GNPs) coated with bistolane-based mesogenic groups were studied. The GNPs were coated by normal alkyl chains and the fluorescent LC compounds, of which the molecular structure was similar to that of the LC matrix. The dispersity of the GNPs in the nematic LC was investigated by polarizing optical microscopy (POM). In order to improve the ER effect of the composite, a simple strategy was investigated from the viewpoint of a material design in surface-modified GNPs by lateral substitution of the mesogenic groups. The presence of the GNPs in the nematic LC led to a slightly enhanced ER effect compared to that observed for only the nematic LC. This study demonstrates the potential of a hybrid system consisting of LCs and GNPs to yield a larger ER effect.
Plasma Arginase-1 Level Is Associated with the Mental Status of Outpatients with Chronic Liver Disease
Authors: Ogino Noriyoshi , Ikeda Fusao , Namba Shihoko , Ohkubo Shinnosuke , Nishimura Tomoaki , Okada Hiroyuki , Hirohata Satoshi , Suganuma Narufumi , Ogino Keiki
Published: February 2021

While plasma arginase-1 has been suggested as a biomarker of mental status in healthy individuals, it has not been evaluated in patients with chronic liver disease. This cross-sectional study investigated the utility of plasma arginase-1 for screening mental status in patients with chronic liver disease. This study included outpatients with chronic liver disease who underwent regular check-ups at Okayama University Hospital between September 2018 and January 2019. In addition to the standard blood tests, the plasma arginase-1 level was analyzed. The patients’ mental status was assessed using the Japanese version of the General Health Questionnaire-28 (GHQ-28). The associations between mental status and various parameters, including plasma arginase-1, were investigated using logistic regression analysis. Among 114 participating patients, 8 were excluded, comprising 6 with insufficient blood samples for plasma arginase-1 measurement and 2 with incomplete questionnaires. Multivariate binomial logistic regression analysis revealed that plasma arginase-1 was significantly and negatively associated with the GHQ-total score, especially somatic symptoms. Therefore, plasma arginase-1 may be a useful biomarker for assessing the mental status of outpatients with chronic liver disease.
Feasibility of Total Variation Noise Reduction Algorithm According to Various MR-Based PET Images in a Simultaneous PET/MR System: A Phantom Study
Authors: Park Chan-Rok , Kang Seong-Hyeon , Lee Young-Jin
Published: February 2021

Recently, the total variation (TV) algorithm has been used for noise reduction distribution in degraded nuclear medicine images. To acquire positron emission tomography (PET) to correct the attenuation region in the PET/magnetic resonance (MR) system, the MR Dixon pulse sequence, which is based on controlled aliasing in parallel imaging, results from higher acceleration (CAIPI; MR-ACDixon-CAIPI) and generalized autocalibrating partially parallel acquisition (GRAPPA; MR-ACDixon-GRAPPA) algorithms are used. Therefore, this study aimed to evaluate the image performance of the TV noise reduction algorithm for PET/MR images using the Jaszczak phantom by injecting 18F radioisotopes with PET/MR, which is called mMR (Siemens, Germany), compared with conventional noise-reduction techniques such as Wiener and median filters. The contrast-to-noise (CNR) and coefficient of variation (COV) were used for quantitative analysis. Based on the results, PET images with the TV algorithm were improved by approximately 7.6% for CNR and decreased by approximately 20.0% for COV compared with conventional noise-reduction techniques. In particular, the image quality for the MR-ACDixon-CAIPI PET image was better than that of the MR-ACDixon-GRAPPA PET image. In conclusion, the TV noise-reduction algorithm is efficient for improving the PET image quality in PET/MR systems.
Analysis of a Wide Voltage Hybrid Soft Switching Converter
Authors: Lin Bor-Ren , Liu Yen-Chun
Published: February 2021

A hybrid PWM converter is proposed and investigated to realize the benefits of wide zero-voltage switching (ZVS) operation, wide voltage input operation, and low circulating current for direct current (DC) wind power conversion and solar PV power conversion applications. Compared to the drawbacks of high freewheeling current and hard switching operation of active devices at the lagging-leg of conventional full bridge PWM converter, a three-leg PWM converter is studied to have wide input-voltage operation (120–600 V). For low input-voltage condition (120–270 V), two-leg full bridge converter with lower transformer turns ratio is activated to control load voltage. For high input-voltage case (270–600 V), PWM converter with higher transformer turns ratio is operated to regulate load voltage. The LLC resonant converter is connecting to the lagging-leg switches in order to achieve wide load range of soft switching turn-on operation. The high conduction losses at the freewheeling state on conventional full bridge converter are overcome by connecting the output voltage of resonant converter to the output rectified terminal of full bridge converter. Hence, a 5:1 (600–120 V) hybrid converter is realized to have less circulating current loss, wide input-voltage operation and wide soft switching characteristics. An 800 W prototype is set up and tested to validate the converter effectiveness.
Efficiency Enhancement on Hybrid Power System Composed of Irreversible Solid Oxide Fuel Cell and Stirling Engine by Finite Time Thermodynamics
Authors: Lai Hsin-Yi , Li Yi-Ting , Chan Yen-Hsin
Published: February 2021

This paper presents the work for efficiency enhancement on a hybrid power system with an irreversible Solid Oxide Fuel Cell (SOFC) and Stirling Engine (SE) for various system design using the approach of finite-time thermodynamics. The SOFC-based cogeneration system was integrated with an SE and several heat components. The effects of design configurations using various interface components on system performance were investigated. By analyzing the SE with finite-time thermodynamics and considering multiple irreversible factors of output power given by the SOFC, the efficiency of the calculation can be more practical and accurate. In this study, the working efficiency of the proposed hybrid system was enhanced by 16.37% compared to that of the conventional system at an intermediate temperature of 873 K. The design approach proposed herein is considered an essential package for building highly efficient power systems working in the intermediate temperature range.
Control of the Transformer Phase Powers Using a Single-Phase Voltage Source
Authors: Drabek Tomasz , Dybowski Paweł
Published: February 2021

Power flow in three-phase distribution grids containing single-phase prosumer voltage sources strongly depends on the RMS value of the voltage of these sources and their phase shifts in relation to the grid voltage. The ideal way to control single-phase prosumer sources should guarantee no return active power to the MV grid through a distribution transformer and no additional reactive power flows in the LV grid. This means that the active power of the one-phase voltage source is consumed by other single-phase customers (in the same phase or in other phases) and the reactive power of this source is equal to zero. The paper presents the results of the investigations of the dynamic control system of a single-phase voltage source that allows meeting these conditions. On the basis of steady-state calculations, the static characteristics of the above-mentioned control, needed to determine of the proper working point of a prosumer source were also obtained. The control process involves the control of the RMS value and phase angle of the voltage source against the phase voltage of the LV grid, to which the source is connected, with simultaneous control of the current phase angle issued by the power source against voltage. The result of the research is the confirmation of the necessity of using a zig–zag connection of the secondary side of distribution transformers. The developed control system of the prosumer voltage source does not fully control the active power of individual phases of the distribution transformer. The paper shows that the power losses in a distribution transformer strongly depend not only on the active power of the prosumer source, but also on its effective voltage and phase in relation to the transformer voltage.
Developing On-Road NOx Emission Factors for Euro 6b Light-Duty Diesel Trucks in Korean Driving Conditions
Authors: Ro Seungcheon , Park Junhong , Shin Myunghwan , Lee Jongtae
Published: February 2021

This study aimed to develop on-road NOx emission factors for Euro 6b light-duty diesel trucks (LDDTs) in Korea. On-road NOx emissions were measured using portable emissions measurement systems and compared with those measured using the Korean Driving Cycle (KDC), the conventional laboratory test used to develop emission factors. To ensure the representativeness of the LDDTs emission factors, five vehicles of three models were driven along two real driving routes for total traveled mileage of 2280 km. On-road NOx levels were 2.1 to 6.9 times higher on average than those measured using the KDC because the latter does not cover the wide variability in vehicle speed and relative positive acceleration, common in real driving conditions. The lean-NOx trap was found to have disappointingly low NOx reduction efficiency in on-road driving. The on-road NOx emission factors by vehicle speeds developed in this study were comparable to the COPERT 4 factors.
Sensory Analysis and Consumer Research in New Meat Products Development
Authors: Ruiz-Capillas Claudia , Herrero Ana M. , Pintado Tatiana , Delgado-Pando Gonzalo
Published: February 2021

This review summarises the main sensory methods (traditional techniques and the most recent ones) together with consumer research as a key part in the development of new products, particularly meat products. Different types of sensory analyses (analytical and affective), from conventional methods (Quantitative Descriptive Analysis) to new rapid sensory techniques (Check All That Apply, Napping, Flash Profile, Temporal Dominance of Sensations, etc.) have been used as crucial techniques in new product development to assess the quality and marketable feasibility of the novel products. Moreover, an important part of these new developments is analysing consumer attitudes, behaviours, and emotions, in order to understand the complex consumer–product interaction. In addition to implicit and explicit methodologies to measure consumers’ emotions, the analysis of physiological responses can also provide information of the emotional state a food product can generate. Virtual reality is being used as an instrument to take sensory analysis out of traditional booths and configure conditions that are more realistic. This review will help to better understand these techniques and to facilitate the choice of the most appropriate at the time of its application at the different stages of the new product development, particularly on meat products.
Improvement of the Target Sensitivity in DECIGO by Optimizing Its Parameters for Quantum Noise Including the Effect of Diffraction Loss
Authors: Ishikawa Tomohiro , Iwaguchi Shoki , Michimura Yuta , Ando Masaki , Yamada Rika , Watanabe Izumi , Nagano Koji , Akutsu Tomotada , Komori Kentaro , Musha Mitsuru , Naito Takeo , Morimoto Taigen , Kawamura Seiji
Published: February 2021

The DECi-hertz Interferometer Gravitational-wave Observatory (DECIGO) is the future Japanese, outer space gravitational wave detector. We previously set the default design parameters to provide a good target sensitivity to detect the primordial gravitational waves (GWs). However, the updated upper limit of the primordial GWs by the Planck observations motivated us toward further optimization of the target sensitivity. Previously, we had not considered optical diffraction loss due to the very long cavity length. In this paper, we optimize various DECIGO parameters by maximizing the signal-to-noise ratio (SNR) of the primordial GWs to quantum noise, including the effects of diffraction loss. We evaluated the power spectrum density for one cluster in DECIGO utilizing the quantum noise of one differential Fabry–Perot interferometer. Then we calculated the SNR by correlating two clusters in the same position. We performed the optimization for two cases: the constant mirror-thickness case and the constant mirror-mass case. As a result, we obtained the SNR dependence on the mirror radius, which also determines various DECIGO parameters. This result is the first step toward optimizing the DECIGO design by considering the practical constraints on the mirror dimensions and implementing other noise sources.
Fast and Large Shrinking of Thermoresponsive Hydrogels with Phase-Separated Structures
Authors: Chung Taehun , Han Im Kyung , Han Jihoon , Ahn Kyojin , Kim Youn Soo
Published: February 2021

Thermoresponsive poly(N-isopropylacrylamide) (PNIPAAm) hydrogels have been attracting attention in a variety of functional materials, such as biomaterials, because they exhibit a volume phase transition phenomenon near physiological temperatures. However, the slow kinetics and small volume shrinkage of bulk PNIPAAm hydrogels upon heating greatly limit their practical application. Here, we report PNIPAAm hydrogels with phase-separated structures that exhibited ultrafast shrinking upon heating. The phase separation into a PNIPAAm-rich phase and a water-rich phase was formed through aqueous polymerization in the presence of NaClO4 salt. Through structural analysis of the hydrogels, a topologically heterogeneous and porous structure was observed, which was highly dependent on the NaClO4 concentration in the polymerization step. Compared to conventional PNIPAAm hydrogels, the phase-separated hydrogels exhibited much faster and larger shrinkage upon heating. Simultaneously, the hydrogels quickly released a large amount of water owing to the effective water channels inside them. The present method can be widely applied to general hydrogels, and it can address the numerous limitations of hydrogels in terms of operating programmability and deformation efficiency.
Adjunctive Nutraceutical Therapies for COVID-19
Authors: Subedi Lalita , Tchen Stephanie , Gaire Bhakta Prasad , Hu Bingren , Hu Kurt
Published: February 2021

The novel coronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2/COVID-19), is a worldwide pandemic, as declared by the World Health Organization (WHO). It is a respiratory virus that infects people of all ages. Although it may present with mild to no symptoms in most patients, those who are older, immunocompromised, or with multiple comorbidities may present with severe and life-threatening infections. Throughout history, nutraceuticals, such as a variety of phytochemicals from medicinal plants and dietary supplements, have been used as adjunct therapies for many disease conditions, including viral infections. Appropriate use of these adjunct therapies with antiviral proprieties may be beneficial in the treatment and/or prophylaxis of COVID-19. In this review, we provide a comprehensive summary of nutraceuticals, such as vitamins C, D, E, zinc, melatonin, and other phytochemicals and function foods. These nutraceuticals may have potential therapeutic efficacies in fighting the threat of the SARS-CoV-2/COVID-19 pandemic.
Effect of Ultra-Micronized-Palmitoylethanolamide and Acetyl-l-Carnitine on Experimental Model of Inflammatory Pain
Authors: Ardizzone Alessio , Fusco Roberta , Casili Giovanna , Lanza Marika , Impellizzeri Daniela , Esposito Emanuela , Cuzzocrea Salvatore
Published: February 2021

Palmitoylethanolamide (PEA), a fatty acid amide, has been widely investigated for its analgesic and anti-inflammatory properties. The ultra-micronized formulation of PEA (um-PEA), that has an enhanced rate of dissolution, is extensively used. Acetyl-l-carnitine (LAC), employed for the treatment of neuropathic pain in humans, is able to cause analgesia by up-regulating type-2 metabotropic glutamate (mGlu2) receptors. In the present study, we tested different associations of um-PEA, LAC and non-micronized PEA (non-m-PEA) in a rat model of carrageenan (CAR)-induced paw edema. Intraplantar injection of CAR into the hind paw of animals caused edema, thermal hyperalgesia, accumulation of infiltrating inflammatory cells and augmented myeloperoxidase (MPO) activity. All these parameters were decreased in a significantly manner by oral administration of a compound constituted by a mixture of um-PEA and LAC in relation 1:1 (5 mg/kg), but not with the association of single compounds administered one after the other. These findings showed the superior anti-inflammatory and anti-nociceptive action displayed by oral administration of um-PEA and LAC versus LAC plus, separate but consecutive, um-PEA in the rat paw CAR model of inflammatory pain.
The Oral Microbiota Changes in Orthodontic Patients and Effects on Oral Health: An Overview
Authors: Contaldo Maria , Lucchese Alberta , Lajolo Carlo , Rupe Cosimo , Di Stasio Dario , Romano Antonio , Petruzzi Massimo , Serpico Rosario
Published: February 2021

Nowadays, there is a considerable interest to study the biological and microbiological changes that accompany orthodontic treatment. Growing knowledge on oral microbiota allows, day after day, to identify and characterize the microbial arrangements specifically associated with oral and extra-oral conditions. The aim of the present work is to highlight any further correlations between orthodontic appliances and the qualitative and quantitative modifications of the oral microbiota, such as predisposing factors for the onset of caries, periodontal diseases, and other infections, which can impact the oral and systemic health of the orthodontic patients. When compared with subjects without orthodontic appliances, orthodontic patients reported significant qualitative and quantitative differences in supra- and subgingival plaque during the entire treatment period. Certain components of fixed appliances (mainly bonded molar brackets, ceramic brackets, and elastomeric ligatures) showed high risks of periodontal disease and tooth decay for patients. An unclear prevalence of Candida spp. and the paucity of studies on viruses and protozoas in the oral microbiota of orthodontic patients need to be further investigated. The evidence emerging from this study could guide clinicians in modulating the timing of controls and enhance patient motivation to prevent the formation of mature plaque, thus reducing the risks of oral-plaque-related diseases.
Triple Monitoring May Avoid Intraneural Injection during Interscalene Brachial Plexus Block for Arthroscopic Shoulder Surgery: A Prospective Preliminary Study
Authors: Pascarella Giuseppe , Strumia Alessandro , Costa Fabio , Rizzo Stefano , Del Buono Romualdo , Remore Luigi Maria , Bruno Federica , Agrò Felice Eugenio
Published: February 2021

Nerve injury is a feared complication of peripheral nerve blockade. The aim of this study was to test the effectiveness of a triple monitoring (TM), i.e., a combination of ultrasound (US), nerve stimulation (NS) and opening injection pressure (OIP) during interscalene brachial plexus block (IBPB) for surgery of the shoulder. Sixty patients undergoing IBPB for shoulder arthroscopy received TM. BSmart®, an inline injection device connected to a 10 mL syringe, was used to detect OIP during IBPB. Nerve stimulation was set to 0.5 mA to rule out any motor response, and if OIP was below 15 PSI, 10 mL of local anaesthetic was injected under US guidance between the C5 and C6 roots. The main outcome was the ability of TM to detect a needle–nerve contact. Other outcomes including the duration of IBPB; pain during injection; postoperative neurologic dysfunction. Triple monitoring revealed needle–nerve contact in 33 patients (55%). In 18 patients, NS evoked motor responses despite first control with US; in a further 15 patients, BSmart® detected an OIP higher than 15 PSI, despite the absence of motor response to NS. Mean duration of IBPB was 67.2 ± 5.3 seconds; neither pain during injection nor postoperative neurologic dysfunctions were detected. Clinical follow up excluded the presence of postoperative neuropathies. Triple monitoring showed to be a useful and feasible tool while performing IBPB for arthroscopic shoulder surgery. Future studies will be needed to confirm our findings.
Impaired Kidney Function Associated with Increased Risk of Side Effects in Patients with Small Vessel Vasculitis Treated with Rituximab as an Induction Therapy
Authors: Rymarz Aleksandra , Matyjek Anna , Sułek-Jakóbczyk Magdalena , Mosakowska Magdalena , Niemczyk Stanisław
Published: February 2021

Rituximab (RTX), a monoclonal antibody against the CD20 molecule, is used as an induction therapy in the treatment of small vessel vasculitis (SVV). The aim of the study was to evaluate the efficacy and safety of RTX induction therapy for refractory SVV. A retrospective analysis of 20 patients treated with RTX for active SVV (BVAS/WG ≥ 3) was performed to assess the remission rate and the drug-related severe adverse events 6 months after therapy. The mean age of the studied population was 49 ± 13 years (50% female), 90% of which were PR3-ANCA positive. Complete remission was achieved in 85% of patients, and partial remission was achieved in a further 10% within 6 months after RTX infusions. The remission rate was not influenced by kidney function. Adverse events such as infections (25%), a late onset of neutropenia (10%) and severe hypogammaglobulinemia (5%) were noted. The patients who developed adverse events were older (42 ± 11 vs. 57 ± 12 years; p = 0.014) and had a higher serum creatinine level (1.3 mg/dL vs. 3.35 mg/dL; p = 0.044). Patients with a glomerular filtration rate (eGFR) lower than 30 mL/min/1.73 m2 had a nine-fold higher risk of side effects (OR 9.0, 95%CI: 1.14–71.0). In conclusion, RTX was highly effective as an induction therapy in patients with SVV. Advanced kidney failure with an eGFR lower than 30 mL/min/1.73 m2 was one of the risk factors for the occurrence of side effects.
Primary Immune Thrombocytopenia: Novel Insights into Pathophysiology and Disease Management
Authors: Singh Anurag , Uzun Günalp , Bakchoul Tamam
Published: February 2021

Immune thrombocytopenia (ITP) is an autoimmune disorder defined by a significantly reduced number of platelets in blood circulation. Due to low levels of platelets, ITP is associated with frequent bruising and bleeding. Current evidence suggests that low platelet counts in ITP are the result of multiple factors, including impaired thrombopoiesis and variations in immune response leading to platelet destruction during pathological conditions. Patient outcomes as well as clinic presentation of the disease have largely been shown to be case-specific, hinting towards ITP rather being a group of clinical conditions sharing common symptoms. The most frequent characteristics include dysfunction in primary haemostasis and loss of immune tolerance towards platelet as well as megakaryocyte antigens. This heterogeneity in patient population and characteristics make it challenging for the clinicians to choose appropriate therapeutic regimen. Therefore, it is vital to understand the pathomechanisms behind the disease and to consider various factors including patient age, platelet count levels, co-morbidities and patient preferences before initiating therapy. This review summarizes recent developments in the pathophysiology of ITP and provides a comprehensive overview of current therapeutic strategies as well as potential future drugs for the management of ITP.
Determining Residual Deviation and Analysis of the Current Use of the Magnetic Compass
Authors: Androjna Andrej , Belev Blagovest , Pavic Ivica , Perkovič Marko
Published: February 2021

The use of electronic compasses and satellite systems has led to the magnetic compass becoming a secondary means of navigation. Yet this means of navigating is not only not obsolete, it is a necessary backup device: the construction simplicity of the magnetic compass, without electrical windings, rotating elements, and control units, remains resistant to power losses, hardware malfunction, and thus is reliable under the harshest conditions. This durability, however, comes at some cost; the magnetic compass is influenced by ships’ permanent and transient magnetism, cargo gears. For the proper use of a magnetic compass, it is necessary to perform an adjustment to determine the residual deviation at regular intervals. The paper analyses selected methods to manage this, and to identify the main features of classical methods. The research was supplemented by a study carried out during the practical compensations of the magnetic compass at sea and by a survey among navigation officers on its basic requirements for proper use. The results indicate insufficient inspection of the magnetic compass. Further, an investigation into the causes of deviation delivers information regarding the causes under varying conditions including type of ship and latitudinal circumstances. This paper presents findings and recommendations to improve the compensation and use of the ships magnetic compasses.
PET/CT Imaging for Personalized Management of Infectious Diseases
Authors: Pijl Jordy P. , Kwee Thomas C. , Slart Riemer H. J. A. , Glaudemans Andor W. J. M.
Published: February 2021

Positron emission tomography combined with computed tomography (PET/CT) is a nuclear imaging technique which is increasingly being used in infectious diseases. Because infection foci often consume more glucose than surrounding tissue, most infections can be diagnosed with PET/CT using 2-deoxy-2-[18F]fluoro-D-glucose (FDG), an analogue of glucose labeled with Fluorine-18. In this review, we discuss common infectious diseases in which FDG-PET/CT is currently applied including bloodstream infection of unknown origin, infective endocarditis, vascular graft infection, spondylodiscitis, and cyst infections. Next, we highlight the latest developments within the field of PET/CT, including total body PET/CT, use of novel PET radiotracers, and potential future applications of PET/CT that will likely lead to increased capabilities for patient-tailored treatment of infectious diseases.
Methodological Tools for Investment Risk Assessment for the Companies of Real Economy Sector
Authors: Zholonko Tetiana , Grebinchuk Olesia , Bielikova Maryna , Kulynych Yurii , Oviechkina Olena
Published: February 2021

Methodological approaches to investing in companies and reducing the negative impact of risks that are formed at the macro and micro levels are considered in the article. The algorithm for expressing investment risks through related risks and conducting an investment risk assessment as a group process is defined. It has been determined that the defining features of investment risks are the environment, duration, and scope of the project, risk position, profile, risk appetite, consequences, capacity, and results of the impact on the investment project. An investment risk accounting system is formed, which is represented by a set of organized structural elements that perform functions related to planning and implementation of a set of measures that identify, assess, monitor, and control risks to minimize negative consequences and enhance opportunities. A method of forming a real portfolio of investment projects considering the dynamic risk factor has been developed.
Structures and Divergent Mechanisms in Capsid Maturation and Stabilization Following Genome Packaging of Human Cytomegalovirus and Herpesviruses
Authors: Muller Clotilde , Alain Sophie , Baumert Thomas F. , Ligat Gaëtan , Hantz Sébastien
Published: February 2021

Herpesviruses are the causative agents of several diseases. Infections are generally mild or asymptomatic in immunocompetent individuals. In contrast, herpesvirus infections continue to contribute to significant morbidity and mortality in immunocompromised patients. Few drugs are available for the treatment of human herpesvirus infections, mainly targeting the viral DNA polymerase. Moreover, no successful therapeutic options are available for the Epstein–Barr virus or human herpesvirus 8. Most licensed drugs share the same mechanism of action of targeting the viral polymerase and thus blocking DNA polymerization. Resistances to antiviral drugs have been observed for human cytomegalovirus, herpes simplex virus and varicella-zoster virus. A new terminase inhibitor, letermovir, recently proved effective against human cytomegalovirus. However, the letermovir has no significant activity against other herpesviruses. New antivirals targeting other replication steps, such as capsid maturation or DNA packaging, and inducing fewer adverse effects are therefore needed. Targeting capsid assembly or DNA packaging provides additional options for the development of new drugs. In this review, we summarize recent findings on capsid assembly and DNA packaging. We also described what is known about the structure and function of capsid and terminase proteins to identify novels targets for the development of new therapeutic options.
Effect of Soft X-ray Irradiation on Film Properties of a Hydrogenated Si-Containing DLC Film
Authors: Kanda Kazuhiro , Imai Ryo , Tanaka Shotaro , Suzuki Shuto , Niibe Masahito , Hasegawa Takayuki , Suzuki Tsuneo , Akasaka Hiroki
Published: February 2021

The effect of soft X-ray irradiation on hydrogenated silicon-containing diamond-like carbon (Si-DLC) films intended for outer space applications was investigated by using synchrotron radiation (SR). We found that the reduction in film thickness was about 60 nm after 1600 mA·h SR exposure, whereas there was little change in their elemental composition. The reduction in volume was attributable to photoetching caused by SR, unlike the desorption of hydrogen in the case of exposure of hydrogenated DLC (H-DLC) film to soft X-rays. The ratio of the sp2 hybridization carbon and sp3 hybridization carbon in the hydrogenated Si-DLC films, sp2/(sp2 + sp3) ratio, increased rapidly from ~0.2 to ~0.5 for SR doses of less than 20 mA·h. SR exposure significantly changed the local structure of carbon atoms near the surface of the hydrogenated Si-DLC film. The rate of volume reduction in the irradiated hydrogenated Si-DLC film was 80 times less than that of the H-DLC film. Doping DLC film with Si thus suppresses the volume reduction caused by exposure to soft X-rays.
Data Augmentation and Feature Selection for Automatic Model Recommendation in Computational Physics
Authors: Daniel Thomas , Casenave Fabien , Akkari Nissrine , Ryckelynck David
Published: February 2021

Classification algorithms have recently found applications in computational physics for the selection of numerical methods or models adapted to the environment and the state of the physical system. For such classification tasks, labeled training data come from numerical simulations and generally correspond to physical fields discretized on a mesh. Three challenging difficulties arise: the lack of training data, their high dimensionality, and the non-applicability of common data augmentation techniques to physics data. This article introduces two algorithms to address these issues: one for dimensionality reduction via feature selection, and one for data augmentation. These algorithms are combined with a wide variety of classifiers for their evaluation. When combined with a stacking ensemble made of six multilayer perceptrons and a ridge logistic regression, they enable reaching an accuracy of 90% on our classification problem for nonlinear structural mechanics.
Assessment the Partial Welding Influences on Fatigue Life of S700MC Steel Fillet Welds
Authors: Moravec Jaromir , Sobotka Jiri , Novakova Iva , Bukovska Sarka
Published: February 2021

Fine-grained steels belonging to the HSLA group (High-Strength Low-Alloy steels) of steels are becoming increasingly popular and are used in both statically and dynamically stressed structures. Due to the method of their production, and thus also the method use to obtain the required mechanical properties, it is really necessary to limit the heat input values for these steels during welding. When applying temperature cycles, HSLA steels in highly heated heat-affected zones (HAZ) reveal intensive grain coarsening and also softening behaviour. This subsequently results in changes in both mechanical and brittle-fracture properties, and the fatigue life of welded joints. While grain coarsening and structure softening have a major effect on the change of strength properties and KCV (Charpy V-notch impact toughness) values of statically stressed welded joints, the effect of these changes on the fatigue life of cyclically stressed welded joints has not yet been quantified. The paper is therefore conceived so as to make it possible to assess and determine the percentage impact of individual aspects of the welding process on changes in their fatigue life. To be more specific, the partial effects of angular deformation, changes that occur in the HAZ of weld, and the notch effect due to weld geometry are assessed.
Laser Powder Bed Fusion (PBF-LB/M) Process Strategies for In-Situ Alloy Formation with High-Melting Elements
Authors: Huber Florian , Rasch Michael , Schmidt Michael
Published: February 2021

In-situ alloy formation by Laser Powder Bed Fusion (PBF-LB/M) from mixtures of easily available elemental powders is an appealing approach for developing and qualifying new alloys for laser based additive manufacturing of metals. However, especially when dealing with high-melting elements, like W, Ta, Mo, or Nb, it is difficult to achieve a homogeneous element distribution and a complete fusion of the powder particles. The aim of this work was to understand the effects of the PBF-LB/M process parameters (laser power, scan speed, laser spot diameter) and three different single- and double-exposure strategies on the fusion of high-melting W, Ta, Mo, and Nb particles in a Ti-matrix. For this purpose, 220 samples with 10 vol.% of the high-melting particle fraction were prepared and analyzed by optical light microscopy and automated image processing, as well as by scanning electron microscopy (SEM). The results are discussed in the context of current research on the process dynamics of PBF-LB/M. Based on that process strategies to support a complete fusion of high-melting particles during in-situ alloy formation are derived. It is shown that the number of unmolten particles can be at least decreased by a factor of ten compared to the most unfavorable parameter combination. For the lower melting elements, Nb and Mo, a complete fusion without any remaining particles visible in the microsections was achieved for certain parameter combinations. The results prove the feasibility of in-situ alloy formation with high-melting alloying elements, but they also demonstrate the necessity to adjust the PBF-LB/M process strategy to achieve a complete dissolution of the alloying elements.
Insights on Molecular Characteristics of Hydrochars by 13C-NMR and Off-Line TMAH-GC/MS and Assessment of Their Potential Use as Plant Growth Promoters
Authors: Fregolente Laís G. , dos Santos João Vitor , Vinci Giovanni , Piccolo Alessandro , Moreira Altair B. , Ferreira Odair P. , Bisinoti Márcia C. , Spaccini Riccardo
Published: February 2021

Hydrochar is a carbon-based material that can be used as soil amendment. Since the physical-chemical properties of hydrochar are mainly assigned to process parameters, we aimed at evaluating the organic fraction of different hydrochars through 13C-NMR and off-line TMAH-GC/MS. Four hydrochars produced with sugarcane bagasse, vinasse and sulfuric or phosphoric acids were analyzed to elucidate the main molecular features. Germination and initial growth of maize seedlings were assessed using hydrochar water-soluble fraction to evaluate their potential use as growth promoters. The hydrochars prepared with phosphoric acid showed larger amounts of bioavailable lignin-derived structures. Although no differences were shown about the percentage of maize seeds germination, the hydrochar produced with phosphoric acid promoted a better seedling growth. For this sample, the greatest relative percentage of benzene derivatives and phenolic compounds were associated to hormone-like effects, responsible for stimulating shoot and root elongation. The reactions parameters proved to be determinant for the organic composition of hydrochar, exerting a strict influence on molecular features and plant growth response.
TEMPO-Oxidized Cellulose Beads as Potential pH-Responsive Carriers for Site-Specific Drug Delivery in the Gastrointestinal Tract
Authors: Xie Fan , De Wever Pieter , Fardim Pedro , Van den Mooter Guy
Published: February 2021

The development of controlled drug delivery systems based on bio-renewable materials is an emerging strategy. In this work, a controlled drug delivery system based on mesoporous oxidized cellulose beads (OCBs) was successfully developed by a facile and green method. The introduction of the carboxyl groups mediated by the TEMPO(2,2,6,6-tetramethylpiperidine-1-oxyradical)/NaClO/NaClO2 system presents the pH-responsive ability to cellulose beads, which can retain the drug in beads at pH = 1.2 and release at pH = 7.0. The release rate can be controlled by simply adjusting the degree of oxidation to achieve drug release at different locations and periods. A higher degree of oxidation corresponds to a faster release rate, which is attributed to a higher degree of re-swelling and higher hydrophilicity of OCBs. The zero-order release kinetics of the model drugs from the OCBs suggested a constant drug release rate, which is conducive to maintaining blood drug concentration, reducing side effects and administration frequency. At the same time, the effects of different model drugs and different drug-loading solvents on the release behavior and the physical state of the drugs loaded in the beads were studied. In summary, the pH-responsive oxidized cellulose beads with good biocompatibility, low cost, and adjustable release rate have shown great potential in the field of controlled drug release.