bluuebunny/crossref_metadata_2025 huggingface dataset:
INSPEKTORAT PROVINSI SULAWESI TENGGARA: APAKAH TIME BUDGET PRESSURE DAN KECERMATAN PROFESIONAL MENGINDIKASI KUALITAS AUDIT?
Authors: Arifuddin  , Safaruddin  , Renata Rasjid Sherly
Published: September 2024

Jumlah pemantauan yang ditemukan meningkat dari 143 kejadian pada tahun 2019 menjadi 254 kejadian pada tahun 2020, menggambarkan tugas yang dihadapi oleh auditor Inspektorat Provinsi Sulawesi Tenggara yang dianggap cukup berat. Penelitian ini bertujuan untuk menguji dan menganalisis pengaruh time budget pressure dan kecermatan profesional terhadap kualitas audit. Penelitian ini menggunakan teknik pengambilan sampling jenuh dengan jumlah sampel sebanyak 33 dari Inspektorat Provinsi Sulawesi Tenggara. Data dikumpulkan melalui kuesioner dan dianalisis menggunakan metode regresi linear berganda dengan bantuan software IBM SPSS Statistics Versi 26. Hasil penelitian menunjukkan bahwa pertama, time budget pressure memiliki pengaruh signifikan terhadap kualitas audit. Kedua, kecermatan profesional memiliki pengaruh signifikan terhadap kualitas audit. Ketiga, baik time budget pressure maupun kecermatan profesional memiliki pengaruh signifikan terhadap kualitas audit.
ANALISIS PERHITUNGAN HARGA POKOK PRODUKSI PADA SEKTOR AGRIKULTUR BERBASIS PSAK 69 DALAM MENENTUKAN HARGA JUAL
Authors: Mirosea Nitri , Aswati Wa  Ode , Wati Indra
Published: September 2024

Usaha karamba baik untuk dikembangkan mengingat besarnya potensi yang dimiliki oleh Kabupaten Wakatobi, namun usaha ini kurang diminati oleh masyarakat karena biaya yang dibutuhkan untuk mendirikan usaha ini sangat besar dan resiko yang dihadapi tinggi. Tujuan penelitian ini adalah untuk mengetahui bagaimana penentuan harga pokok produksi menggunakan metode variable costing dalam menentukan harga jual pada usaha karamba Padallean. Metode analisis yang digunakan dalam penelitian ini adalah metode analisis deskriptif. Hasil penelitian menunjukan bahwa perhitungan harga pokok produksi oleh usaha karamba Padallean lebih tinggi di bandingkan menggunakan metode variable costing. Usaha ini menentukan harga jual menggunakan harga pasar dan belum menggunakan metode perhitungan harga jual cost plus pricing.
Passive Aggression: Explaining The Strategic Logic of Military Attrition
Authors: Li Deye
Published: September 2024

Attrition wars are costly. Why do states choose to wage expected wars of attrition over an ex-ante bargaining settlement? Conventional wisdom suggests that the expectation of defense favorability induces rational deterrence. I propose a novel theory of Passive Aggression to explain the strategic logic of military attrition: the defender can strategically conceal private information about military readiness and organizational effectiveness to prevent the attacker from updating the assessment of the offense-defense balance in crisis bargaining. Under persistent ex-ante military optimism, the rational attacker is likely to blunder. Empirically, my research identifies bait-and-bleed strategies through descriptive inferences from policy actions and strategic deliberations. Then, I use time-slice process tracing as a plausibility probe on the causal link between perceived offense-defense balance and bait-and-bleed. I find that Passive Aggression Theory provides critical insights into explaining the strategic behaviors of the USSR, China, and Vietnam during the unfolding of The Third Indochina War.
QSPRmodeler - An open source application for molecular predictive analytics
Authors: Bachorz Rafał A. , Nowak Damian , Ratajewski Marcin
Published: September 2024

The drug design process can be successfully supported using a variety of in silico methods. Some of these are oriented toward molecular property prediction, which is a key step in the early drug discovery stage. Before experimental validation, drug candidates are usually compared with known experimental data. Technically, this can be achieved using machine learning approaches, in which selected experimental data are used to train the predictive models. The proposed Python software is designed for this purpose. It supports the entire workflow of molecular data processing, starting from raw data preparation followed by molecular descriptor creation and machine learning model training. The predictive capabilities of the resulting models were carefully validated internally and externally. These models can be easily applied to new compounds, including within more complex workflows involving generative approaches.
Nasoseptal chondroprogenitors isolated through fibronectin-adherence confer no biological advantage for cartilage tissue engineering compared to nasoseptal chondrocytes
Authors: Jovic Thomas H. , Thomson Emman J. , Jones Nick , Thornton Catherine A. , Doak Shareen H. , Whitaker Iain S.
Published: September 2024

Introduction

The ability to bioprint facial cartilages could revolutionise reconstructive surgery, but identifying the optimum cell source remains one of the great challenges of tissue engineering. Tissue specific stem cells: chondroprogenitors, have been extracted previously using preferential adhesion to fibronectin based on the expression of CD49e: a perceived chondroprogenitor stem cell marker present on <1% of cartilage cells. This study sought to determine whether these fibronectin-adherent chondroprogenitor cells could be exploited for cartilage tissue engineering applications in isolation, or combined with differentiated chondrocytes.

Methods

Nasoseptal cartilage samples from 20 patients (10 male, 10 female) were digested to liberate cartilage-derived cells (CDCs) from extracellular matrix. Total cell number was counted using the Trypan Blue exclusion assay and added to fibronectin coated plates for 20 min, to determine the proportion of fibronectin-adherent (FAC) and non-adherent cells (NFACs). All populations underwent flow cytometry to detect mesenchymal stem/progenitor cell markers and were cultured in osteogenic, chondrogenic and adipogenic media to determine trilineage differentiation potential. Cell adherence and growth kinetics of the different populations were compared using iCELLigence growth assays. Chondrogenic gene expression was assessed using RT-qPCR for Type 2 collagen, aggrecan and SOX9 genes. Varying proportions of NFAC and FACs were cultured in alginate beads to assess tissue engineering potential.

Results

52.6% of cells were fibronectin adherent in males and 57.7% in females, yet on flow cytometrical analysis, only 0.19% of cells expressed CD49e. Moreover, all cells (CDC, FAC and NFACs) demonstrated an affinity for trilineage differentiation by first passage and the expression of stem/progenitor cell markers increased significantly from digest to first passage (CD29, 44, 49e, 73 and 90, p < 0.0001). No significant differences were seen in adhesion or growth rates. Collagen and aggrecan gene expression was higher in FACs than CDCs (2-fold higher, p = 0.008 and 0.012 respectively), but no differences in chondrogenic potential were seen in any cell mixtures in 3D culture models.

Conclusion

The fibronectin adhesion assay does not appear to reliably isolate a chondroprogenitor cell population from nasoseptal cartilage, and these cells confer no advantageous properties for cartilage tissue engineering. Refinement of cell isolation methods and chondroprogenitor markers is warranted for future nasoseptal cartilage tissue engineering efforts.
Approved drugs successfully repurposed against Leishmania based on machine learning predictions
Authors: Oualha Rafeh , Abdelkrim Yosser Zina , Guizani Ikram , Harigua-Souiai Emna
Published: September 2024

Drug repurposing is a promising approach towards the discovery of novel treatments against Neglected Tropical Diseases, such as Leishmaniases, presenting the advantage of reducing both costs and duration of the drug discovery process. In previous work, our group developed a Machine Learning pipeline for the repurposing of FDA-approved drugs against Leishmania parasites. The present study is focused on an in vitro validation of this approach by assessing the antileishmanial effects of 10 predicted drug candidates. First, we evaluated the drugs’ activity against promastigotes from two strains of L. infantum and one of L. major, which caused distinct clinical manifestations, using an MTT assay. The standard anti-Leishmania drug Amphotericin B was used as a positive control. Five molecules demonstrated anti-Leishmania effects, out of which Acebutolol, Prilocaine and Phenylephrine are described herein for the first time. When tested on promastigote growth, Acebutolol displayed IC50 values ranging from 69.28 to 145.53 µg/mL. Prilocaine exhibited IC50 values between 33.10 and 45.81 µg/mL. Phenylephrine, on the other hand, presented IC50 values >200 µg/mL. The two remaining drugs, Dibucaine and Domperidone, exhibited significantly low IC50 values varying between 0.58 and 1.05 µg/mL, and 6.30 and 8.17 µg/mL, respectively. Both compounds were previously described as anti-Leishmania agents in vivo. All five compounds demonstrated no notable cytotoxic effects on THP-1-derived macrophages at the IC50 concentrations, allowing for their testing on the intracellular form of L. major and L. infantum parasites. Interestingly, all compounds exhibited antileishmanial activity on amastigotes with enhanced IC50 values compared to the corresponding promastigotes. Noticeably, Dibucaine and Domperidone displayed IC50 values of at most 1.99 µg/mL. Acebutolol, Prilocaine and Phenylephrine showed IC50 values ranging from 13.84 to 66.81 µg/mL. Our previously published Computer-Aided repositioning pipelines of FDA-approved drugs as antileishmanial agents identified Dibucaine and Domperidone as candidates in support of previous in vivo studies. This study consolidates such findings through the in vitro validation against 2 Leishmania species, highly prevalent in Africa and Middle East, and reveals Acebutolol, Prilocaine, and Phenylephrine as novel anti-Leishmania effectors, confirming the relevance of our approach and calling for further investigations.
Primary ovarian insufficiency: update on clinical and genetic findings
Authors: Federici Silvia , Rossetti Raffaella , Moleri Silvia , Munari Elisabetta V. , Frixou Maria , Bonomi Marco , Persani Luca
Published: September 2024

Primary ovarian insufficiency (POI) is a disorder of insufficient ovarian follicle function before the age of 40 years with an estimated prevalence of 3.7% worldwide. Its relevance is emerging due to the increasing number of women desiring conception late or beyond the third decade of their lives. POI clinical presentation is extremely heterogeneous with a possible exordium as primary amenorrhea due to ovarian dysgenesis or with a secondary amenorrhea due to different congenital or acquired abnormalities. POI significantly impacts non only on the fertility prospect of the affected women but also on their general, psychological, sexual quality of life, and, furthermore, on their long-term bone, cardiovascular, and cognitive health. In several cases the underlying cause of POI remains unknown and, thus, these forms are still classified as idiopathic. However, we now know the age of menopause is an inheritable trait and POI has a strong genetic background. This is confirmed by the existence of several candidate genes, experimental and natural models. The most common genetic contributors to POI are the X chromosome-linked defects. Moreover, the variable expressivity of POI defect suggests it can be considered as a multifactorial or oligogenic defect. Here, we present an updated review on clinical findings and on the principal X-linked and autosomal genes involved in syndromic and non-syndromic forms of POI. We also provide current information on the management of the premature hypoestrogenic state as well as on fertility preservation in subjects at risk of POI.
Ecological risk assessment and influencing factor analysis of the Yellow River basin based on LUCC and boosted regression tree
Authors: Yan Jixuan , Yao Xiangdong , Li Qiang , Song Miao , Li Jie , Li Guang , Qi Guangping , Qiao Hongqiang , Gao Pengcheng , Zhang Meihua
Published: September 2024

The Yellow River basin is an important ecological security barrier and ecologically vulnerable region in China. Landscape ecological risk assessment and influencing factor analysis based on land-use/land-cover change (LUCC) and boosted regression tree (BRT) models are of great significance to the coordinated and sustainable development of regional ecological environment and social economy. Based on LUCC and driving factor data from 1980 to 2020, the ecological risk index (ERI) model was constructed to evaluate the spatiotemporal evolution characteristics of ecological risk in the past 40 years. Especially, a new method of influencing factor analysis based on the BRT model is proposed. The final index size of the influencing factors was further quantitatively evaluated. The results showed that the spatial distribution pattern of landscape ecological risk in the Yellow River basin was “highest in the north and lowest in the south, highest in the west and lowest in the east.” During the periods, the overall ecological risk and high risk increased first and then decreased. Elevation (24.8%) was the most important factor affecting landscape ecological risk, followed by precipitation (17.8%), GDP (15.2%), and temperature (14.6%). It showed that the particularity of the geographical location of the Yellow River basin eventually led to the stronger influence of natural factors on the change in landscape ecological risk under the interference of human activities. This study will provide a new perspective for quantitative assessment of the final contribution rate of landscape ecological risk factors.
Exploring common pathogenic association between Epstein Barr virus infection and long-COVID by integrating RNA-Seq and molecular dynamics simulations
Authors: Kanwal Ayesha , Zhang Zhiyong
Published: September 2024

The term "Long-COVID" (LC) is characterized by the aftereffects of COVID-19 infection. Various studies have suggested that Epstein–Barr virus (EBV) reactivation is among the significant reported causes of LC. However, there is a lack of in-depth research that could largely explore the pathogenic mechanism and pinpoint the key genes in the EBV and LC context. This study mainly aimed to predict the potential disease-associated common genes between EBV reactivation and LC condition using next-generation sequencing (NGS) data and reported naturally occurring biomolecules as inhibitors. We applied the bulk RNA-Seq from LC and EBV-infected peripheral blood mononuclear cells (PBMCs), identified the differentially expressed genes (DEGs) and the Protein–Protein interaction (PPI) network using the STRING database, identified hub genes using the cytoscape plugins CytoHubba and MCODE, and performed enrichment analysis using ClueGO. The interaction analysis of a hub gene was performed against naturally occurring bioflavonoid molecules using molecular docking and the molecular dynamics (MD) simulation method. Out of 357 common genes, 22 genes (CCL2, CCL20, CDCA2, CEP55, CHI3L1, CKAP2L, DEPDC1, DIAPH3, DLGAP5, E2F8, FGF1, NEK2, PBK, TOP2A, CCL3, CXCL8, DEPDC1, IL6, RETN, MMP2, LCN2, and OLR1) were classified as hub genes, and the remaining ones were classified as neighboring genes. Enrichment analysis showed the role of hub genes in various pathways such as immune-signaling pathways, including JAK-STAT signaling, interleukin signaling, protein kinase signaling, and toll-like receptor pathways associated with the symptoms reported in the LC condition. ZNF and MYBL TF-family were predicted as abundant TFs controlling hub genes' transcriptional machinery. Furthermore, OLR1 (PDB: 7XMP) showed stable interactions with the five shortlisted refined naturally occurring bioflavonoids, i.e., apigenin, amentoflavone, ilexgenin A, myricetin, and orientin compounds. The total binding energy pattern was observed, with amentoflavone being the top docked molecule (with a binding affinity of –8.3 kcal/mol) with the lowest total binding energy of −18.48 kcal/mol. In conclusion, our research has predicted the hub genes, their molecular pathways, and the potential inhibitors between EBV and LC potential pathogenic association. The in vivo or in vitro experimental methods could be utilized to functionally validate our findings, which would be helpful to cure LC or to prevent EBV reactivation.
Treatment and related morbidity of nasal cavity and paranasal sinus cancers
Authors: Smaadahl Nils , Hool Sara-Lynn , Reinhardt Philipp , Mose Lucas , Hohenberger Ralph , Giger Roland , Schanne Daniel Hendrik , Anschuetz Lukas
Published: September 2024

Introduction

Sinonasal malignancies are rare and histologically heterogeneous cancers of the nasal cavity and sinuses. The treatment of choice is usually surgery and, if necessary, adjuvant radiotherapy. In this study, we aimed to investigate treatment modalities and associated morbidity.

Methods

A consecutive case series of solid sinonasal cancer treated at our tertiary referral center was analyzed. We performed a retrospective chart review and statistical analysis.

Results

A total of 156 patients with sinonasal cancer were enrolled in the present study. Male patients were more frequently affected (62%) and the median age was 64 years. Squamous cell carcinoma, adenocarcinoma and malignant melanoma (MM) were the most common histopathological entities. Surgery was the primary treatment modality for 73% of curatively treated patients. Primary radiotherapy alone or in combination with systemic treatment was less frequent. Median overall (OS) and recurrence-free survival (RFS) was 164 months and 71.3 months, respectively. Multivariate analysis revealed negative associations of histology (MM) and skull base involvement on RFS and age, skull base involvement and the type of primary therapy (radiochemotherapy) on OS. Postoperative 30-day morbidity was low, with most patients (84%) experiencing no reported events. Radiotherapy was generally well-tolerated, despite most of patients experienced acute toxicity such as dermatitis (80.6%) or mucositis (72.1%). However, only one event of acute toxicity > grade 3 was reported. Long term morbidity was most frequently reported as pain (23%), dry mucosa (19%) and anosmia (14%).

Conclusion

We observed negative associations of histology (MM) and skull base involvement on RFS and age, skull base involvement and the type of primary therapy (radiochemotherapy) on OS. Acute treatment-related morbidity was generally low for surgical patients and considerable for irradiated patients. Moreover, a consistent part of the cohort displayed long term morbidity.
A systematic review of treatment for impulsivity and compulsivity
Authors: Aguilar-Yamuza Beatriz , Trenados Yolanda , Herruzo Carlos , Pino María José , Herruzo Javier
Published: September 2024

Introduction

The aim of this study was to comprehensively review existing treatments for impulsivity and compulsivity in non-substance addictions, driven by the importance of these factors in addictive disorder development and treatment efficacy.

Methods

A systematic review carried out following PRISMA guidelines identified 14 articles from a total of 764 studies, highlighting the limited literature that is available on psychological treatments for non-substance addictions, in particular studies focusing on impulsivity and compulsivity. The studies were categorized by behavioral addiction type.

Results

For compulsive sexual behavior and problematic pornography use, cognitive-behavioral therapy (CBT) has shown significant improvements in compulsivity. In gambling disorder, interventions like cognitive bias modification and motivational interviewing combined with CBT have been effective in reducing impulsivity. Cognitive-behavioral therapy was advocated for compulsive shopping, although results varied. For problematic internet use, dialectical behavior therapy and acceptance and commitment therapy have been effective in addressing impulsivity. Despite literature supporting CBT for most behavioral addictions, the review noted that some articles indicate that dialectical behavior therapy and acceptance and commitment therapy were also effective for problematic internet use, suggesting different underlying mechanisms for this type of addiction.

Discussion

It also highlighted limitations, including the small number of studies and the lack of standardized assessment measures. Further research is needed to understand underlying mechanisms and develop tailored treatments for impulsivity and compulsivity in non-substance addictions. These findings offer new directions for research and intervention guidelines in behavioral addictions.
Econeurobiology and brain development in children: key factors affecting development, behavioral outcomes, and school interventions
Authors: Mualem Raed , Morales-Quezada Leon , Farraj Rania Hussein , Shance Shir , Bernshtein Dana Hodaya , Cohen Sapir , Mualem Loay , Salem Niven , Yehuda Rivka Riki , Zbedat Yusra , Waksman Igor , Biswas Seema
Published: September 2024

The Econeurobiology of the brain describes the environment in which an individual’s brain develops. This paper explores the complex neural mechanisms that support and evaluate enrichment at various stages of development, providing an overview of how they contribute to plasticity and enhancement of both achievement and health. It explores the deep benefits of enrichment and contrasts them with the negative effects of trauma and stress on brain development. In addition, the paper strongly emphasizes the integration of Gardner’s intelligence types into the school curriculum environment. It emphasizes the importance of linking various intelligence traits to educational strategies to ensure a holistic approach to cognitive development. In the field of Econeurobiology, this work explains the central role of the environment in shaping the development of the brain. It examines brain connections and plasticity and reveals the impact of certain environmental factors on brain development in early and mid-childhood. In particular, the six key factors highlighted are an environment of support, nutrition, physical activity, music, sleep, and cognitive strategies, highlighting their potential to improve cognitive abilities, memory, learning, self-regulation, and social and emotional development. This paper also investigates the social determinants of health and education in the context of Econeurobiology. It emphasizes the transformative power of education in society, especially in vulnerable communities facing global challenges in accessing quality education.
Validations of various in-hand object manipulation strategies employing a novel tactile sensor developed for an under-actuated robot hand
Authors: Singh Avinash , Pinto Massimilano , Kaltsas Petros , Pirozzi Salvatore , Sulaiman Shifa , Ficuciello Fanny
Published: September 2024

Prisma Hand II is an under-actuated prosthetic hand developed at the University of Naples, Federico II to study in-hand manipulations during grasping activities. 3 motors equipped on the robotic hand drive 19 joints using elastic tendons. The operations of the hand are achieved by combining tactile hand sensing with under-actuation capabilities. The hand has the potential to be employed in both industrial and prosthetic applications due to its dexterous motion capabilities. However, currently there are no commercially available tactile sensors with compatible dimensions suitable for the prosthetic hand. Hence, in this work, we develop a novel tactile sensor designed based on an opto-electronic technology for the Prisma Hand II. The optimised dimensions of the proposed sensor made it possible to be integrated with the fingertips of the prosthetic hand. The output voltage obtained from the novel tactile sensor is used to determine optimum grasping forces and torques during in-hand manipulation tasks employing Neural Networks (NNs). The grasping force values obtained using a Convolutional Neural Network (CNN) and an Artificial Neural Network (ANN) are compared based on Mean Square Error (MSE) values to find out a better training network for the tasks. The tactile sensing capabilities of the proposed novel sensing method are presented and compared in simulation studies and experimental validations using various hand manipulation tasks. The developed tactile sensor is found to be showcasing a better performance compared to previous version of the sensor used in the hand.
Establishing a clinically applicable frailty phenotype screening tool for aging dogs
Authors: Russell Katharine J. , Mondino Alejandra , Fefer Gilad , Griffith Emily , Saker Korinn , Gruen Margaret E. , Olby Natasha J.
Published: September 2024

Introduction

Frailty is a well-defined clinical syndrome in humans caused by accumulation of impairments which result in loss of reserve capacity and increased vulnerability to disability, dependence, and death. Dogs are of particular interest in studies of frailty due to the similarities they share with people in their environment, lifestyles, and age-related diseases.

Materials and methods

The aim of this study was to develop a frailty phenotype screening tool, based on previously validated measures in dogs, which could be easily applied in the clinical setting, and which was predictive of all-cause, short term (6-month) mortality. The study was conducted in two phases. In phase 1, a retrospective cohort of 51 dogs was used to identify and evaluate potential measures for the five domains of frailty. This information was then used to develop a simple frailty phenotype based on examination findings and owner directed questions. In phase 2 of the study, this phenotype was evaluated in a prospective cohort of 198 dogs aged 9 years or older from multiple different specialty and primary care services to determine how the phenotype performed across a diverse canine population.

Results

The developed frailty phenotype was predictive of all-cause, short-term mortality independent of age, sex, or weight (hazard ratio = 4.71; 95% CI, 2.66–8.8). Of the covariates evaluated only breed was significant, with purebred dogs having 1.85 times higher mortality than mixed breed dogs (95% CI, 1.04–3.31). The frailty phenotype performed similarly across all hospital services from which patients were enrolled.

Conclusion

Based on these findings, the defined frailty phenotype represents a valuable screening tool for early risk identification and intervention, and can aid in clinical decision making for owners and veterinarians. Additionally, it will promote further research into the understanding and treatment of frailty in dogs.
Specific Sources Exert Influence on the Community Structures of Bioaerosols
Authors: Nie Changliang , Qiu Yuqi , Pei Tianxiao , Qin Yunhan
Published: September 2024

Bioaerosols constitute a crucial component of atmospheric particulate matter, encompassing physical and chemical aerosol properties along with biological characteristics. They can influence global ecosystems, climate change dynamics, and air quality. Notably, bioaerosols serve as a significant pathway for transmitting respiratory infectious diseases, garnering widespread attention worldwide following major pandemics such as COVID-19. Thanks to the development of high-throughput sequencing technologies, studies on bioaerosols have flourished in recent years. Understanding the interconnectedness of sources, spatial and temporal distributions, influencing factors, and health risks associated with bioaerosols is imperative for devising pollution mitigation strategies and preventing the spread of related epidemics. This review provides an overview of bioaerosol sources while elucidating distribution patterns within their community structure across various source types. Lastly, this overview offers insights into future advancements in the field of bioaerosols along with corresponding recommendations.
Impact of Farmers’ Participation in the Transformation of the Farmland Transfer Market on the Adoption of Agricultural Green Production Technologies
Authors: Quan Tianshu , Jia Weiguo , Quan Tianli , Xu Yuyun
Published: September 2024

Exploring the adoption of green production technologies by farmers (GTA) is of great significance, given the context of global climate change and sustainable agricultural development. This article starts from the perspective of the transformation of the farmland transfer market, based on research data from 2076 farmers, and uses the endogenous disposal effect model to examine its impact on GTA. The results indicate that the transformation of China’s farmland transfer market has a significant “greening” effect on agriculture. Specifically, the paid transfer of farmland, written leases, and clear lease forms promote GTA, while acquaintance transactions are not conducive to it. Participation in the farmland transfer market has increased the likelihood of GTA by 18.7% and is statistically significant at the 1% level. However, the adoption level of green production technology by ordinary farmers remains low, and achieving green development in agriculture is a long and arduous task. Mechanism analysis shows that the transformation of the farmland transfer market can effectively enhance GTA by increasing the scale of operations, farmers’ income, and mechanization levels. When the transfer targets new business entities, such as family farms, professional cooperatives, and enterprises, it significantly promotes GTA. The findings suggest that deepening the reform of the farmland transfer market, promoting large-scale agricultural operations, strengthening the application of green production technologies, improving the level of agricultural mechanization, and accelerating the process of sustainable agricultural development are all essential steps toward enhancing GTA and, ultimately, achieving sustainable agricultural development.
Impact of Rural E-Commerce on Farmers’ Income and Income Gap
Authors: Guan Xin , He Lei , Hu Zhiquan
Published: September 2024

Rural e-commerce, as a new form of digital economy, is of great significance in promoting the income of rural households and realizing common prosperity. Based on the 2021 China Rural Revitalization Survey (CRRS), the impact of rural e-commerce on farmers’ income and the intra-rural income gap is explored using quantile regression. The results found that (1) rural e-commerce can effectively promote the level of farm household income and alleviate the intra-rural income disparity, with this finding still holding after addressing the potential endogeneity problem and conducting robust-type tests. (2) Rural e-commerce has the most pronounced income-generating effect on low-income households in the southern region; participation in rural e-commerce has a more “center-expanding” effect on households in the secondary education and high material capital groups. (3) Saving production and operation costs, shortening the product circulation chain, and improving access to information are channels through which rural e-commerce affects households’ income. It is recommended to actively promote the deep integration and development of rural e-commerce in rural areas, establish a sound mechanism for bridging the “digital divide”, encourage e-commerce “leaders” to build a perfect industrial chain, and guide smallholders to integrate into the rural e-commerce industrial chain to enjoy the digital dividend.
Mechanism of Eriochloa villosa (Thunb.) Kunth Resistance to Nicosulfuron
Authors: Guo Jing , Xu Zeqian , Jiao Ting , Gao Hong , Wang Yuechao , Zhang Liguo , Li Mukai , Liu Xiaomin , Yan Chunxiu , Han Yujun
Published: September 2024

Eriochloa villosa (Thunb.) Kunth, the main weed in corn fields, has gradually developed resistance to nicosulfuron due to continuous and extensive application. We identified a biotype showing resistance to ALS inhibitor nicosulfuron with a resistant index 13.83, but without any target spot mutation. Herein, transcriptome sequencing was used to analyze the differences in gene expression at the transcriptional level between nicosulfuron-resistant E. villosa HEK-40 varieties and sensitive E. villosa HEK-15 varieties. The resistant and sensitive varieties comparison revealed 9931 DEGs after nicosulfuron application, of which 5426 and 4505 genes were up-regulated and down-regulated, respectively. Some contigs related to metabolic resistance were identified based on differential expression via RNA-Seq, which includes ABC transporters (ko02010), glucosinolate biosynthesis (ko00966), 2-oxocarboxylic acid metabolism (ko01210), alanine, aspartate, and glutamate metabolism pathways (ko00250). Seven CYP450 genes, four GST genes, ten ABC transporter genes, and two GT genes related to metabolic resistance were identified. The 10 candidate genes screened were validated using q-PCR. This validation indicates that activities associated with P450 enzymes, ABC transporters, and glutathione S-transferases (GST) may play a role in conferring resistance, which is important for reducing the impact of weeds on corn fields and ensuring food security.
Genetic Diversity Analysis of Monogerm Cytoplasmic Male Sterile and Maintainer Lines of Sugar Beet
Authors: Chen Pian , Chen Shuyuan , Pi Zhi , Li Shengnan , Wu Zedong
Published: September 2024

Sugar beet is an economically significant crop, and the homozygosity of paired monogerm cytoplasmic male sterile (CMS) and maintainer lines directly influences the number of hybrid combinations that can be created. This study aimed to evaluate the genetic variation within monogerm sugar beet germplasm resources to establish a foundation for advancements in sugar beet breeding and the development of hybrid female parent lines. This study analyzed the genetic diversity of 86 distinct monogerm germplasm resources, including 38 paired monogerm CMS and maintainer lines, 5 individual maintainer lines, and 5 externally introduced sterile lines. The analysis employed 26 pairs of SSR primers and 35 pairs of InDel primers across nine sugar beet chromosomes. Several genetic parameters, and analyses such as structural analysis, genetic diversity analysis, and principal coordinate analysis, were used to evaluate the samples. The results indicated that these strains could be classified into two groups: Group I and Group II. Group I was further divided into three subgroups. Further, 18 pairs of original CMS and maintainer lines were successfully clustered, confirming that their nuclei had achieved homozygosity, making them suitable for use in the development of binary sterile lines. However, 20 other pairs still require further backcrossing to achieve homozygosity. The analysis of molecular variance (AMOVA) revealed that most of the genetic variation occurred within individuals, with relatively low genetic differentiation between groups. Significant genetic differentiation was observed between Subgroups 2 and 3, and between Subgroups 1 and 3. The results suggest that additional monogerm sterile and maintainer lines from these subgroups should be selected to configure binary sterile lines. This study offers a theoretical foundation for developing new sugar beet germplasm resources and cultivating hybrid mother plants.
Medium- and Long-Term Immune Responses in the Small Intestine in Piglets from Oral Vaccination against Escherichia coli
Authors: Miralles Aida , Ramis Guillermo , Pallarés Francisco J. , Párraga-Ros Ester , Seva Juan
Published: September 2024

Post-weaning stress, together with Escherichia coli, are two of the key factors in the occurrence of post-weaning diarrhea. There are different commercial vaccines that induce immunity at the local or systemic level, improving farm health and avoiding economic losses in the pork industry. That is why the objective of this study was to evaluate the effect of an oral enterotoxigenic E. coli F4/F18 vaccine on immunity and intestinal integrity in the middle and long term after inoculation. The gene expression of the biomarkers indicative of cellular infiltration (calprotectin, CAL), tight junction proteins (occludin, OCL; zonulin, ZON; and claudin, CLA) and a panel of proinflammatory (interleukins, IL: IL1α, IL1β, IL6, IL8, IL12p35 and IL12p40; interferons, IFN: IFNα and IFNγ; and tumoral necrosis factor, TNF: TNFα) and anti-inflammatory mediator cytokines (TGFβ and IL10) were analyzed, as well as histomorphology in jejunum and ileum, the cell density of goblet cells, intraepithelial lymphocytes and IgA-producing cells. Differences were observed in ZON, CLA and CAL, with greater gene expression in observed in vaccinated piglets at 42 days post vaccination (dpv) in the ileum. Regarding the expression of cytokines, the vaccinated animals showed significant differences in IL1α, IL6, IL12p35, IL12p40, IFNα, IFNγ, TNFα and TGFβ at 42 dpv in the jejunum or ileum. The villi showed greater height in the vaccinated piglets and the ratio between villus height and crypt depth was significantly greater in the vaccinated group in the jejunum at 84 dpv. The count of IgA-producing cells shows higher values for the unvaccinated group in the ileum, while intraepithelial lymphocytes show a significant increase in both jejunum and ileum in vaccinated piglets. We can conclude that oral vaccination against E. coli produces an evident effect, which manifests itself even in the middle and long term after the challenge, including immune response, decrease in antimicrobials usage, better histological structure in intestine and the improvement of performance.
Insight into the Gut–Brain Axis and the Productive Performance and Egg Quality Response to Kudzu Leaf Flavonoid Supplementation in Late-Laying Hens
Authors: Tang Shi , Hu Yaodong , Luo Jiahui , Hu Meijun , Chen Maolin , Ye Dehan , Ye Jingsong , Xue Fuguang
Published: September 2024

(1) Background: Improving feed efficiency and the vitality of the reproductive system in the late stage of the egg-laying period is of great significance for prolonging the egg-laying cycle and improving egg quality. In the present study, a new flavonoid, which was extracted from kudzu leaf, was chosen to investigate its effects on the productive performance and egg quality of late-laying hens. (2) Methods: A total of 360 500-day-old Hy-Line Brown layer hens were randomly divided into a control treatment group (no KLF supplementation), and groups that received 0.2%, 0.4%, 0.6%, 0.8%, and 1.0% KLF supplement treatments. Each treatment contained 6 replicates, with 10 hens in each replicate. Productive performance metrics, including the daily egg production, egg weight, the number of deformed eggs, egg quality, egg density, egg shape index, eggshell strength, yolk color, and the Haugh unit, were meticulously recorded for each replicate. Furthermore, microbial communities and hypothalamus gene expressions were investigated based on the results of the productive performance and egg quality. (3) Results: KLF supplementation significantly decreased the deformity rate while significantly increasing the eggshell strength in the finishing phase afterward (p < 0.05). Specifically, hens supplemented with 0.6% KLF possessed the lowest deformed egg rate. KLF supplementation significantly increased the relative abundances of Bifidobacterium sp., Blautia sp., Lactococcus sp., and Lactobacillus sp., while significantly decreasing Parasutterella sp. and Escherichia-Shigella sp. (p < 0.05). Furthermore, the interactive analysis showed the hypothalamus gene expression mainly interacted with probiotics, such as Bifidobacterium sp. and Lactobacillus sp., through ribosome biogenesis, nucleocytoplasmic transport, and cAMP signaling pathways. (4) Conclusions: The findings of the present study indicate that KLF supplementation significantly proliferated probiotics, such as Bifidobacterium and Lactobacillus, which may have further interacted with hypothalamus genes, thus decreasing the deformity rate while increasing eggshell strength in the finishing phase.
Antibiotic Use and Antimicrobial Resistance: A Global Public Health Crisis
Authors: Estany-Gestal Ana , Salgado-Barreira Angel , Vazquez-Lago Juan Manuel
Published: September 2024

The discovery of antibiotics revolutionized modern medicine, effectively treating bacterial infections that were once fatal [...]
A Decade of Antimicrobial Resistance in Human and Animal Campylobacter spp. Isolates
Authors: Barata Rita , Saavedra Maria José , Almeida Gonçalo
Published: September 2024

Objectives: Campylobacter spp. remain a leading cause of bacterial gastroenteritis worldwide, with resistance to antibiotics posing significant challenges to treatment and public health. This study examines profiles in antimicrobial resistance (AMR) for Campylobacter isolates from human and animal sources over the past decade. Methods: We conducted a comprehensive review of resistance data from studies spanning ten years, analyzing profiles in resistance to key antibiotics, ciprofloxacin (CIP), tetracycline (TET), erythromycin (ERY), chloramphenicol (CHL), and gentamicin (GEN). Data were collated from various regions to assess global and regional patterns of resistance. Results: The analysis reveals a concerning trend of increasing resistance patterns, particularly to CIP and TET, across multiple regions. While resistance to CHL and GEN remains relatively low, the high prevalence of CIP resistance has significantly compromised treatment options for campylobacteriosis. Discrepancies in resistance patterns were observed between human and animal isolates, with variations across different continents and countries. Notably, resistance to ERY and CHL showed regional variability, reflecting potential differences in antimicrobial usage and management practices. Conclusions: The findings underscore the ongoing challenge of AMR in Campylobacter, highlighting the need for continued surveillance and research. The rising resistance prevalence, coupled with discrepancies in resistance patterns between human and animal isolates, emphasize the importance of a One Health approach to address AMR. Enhanced monitoring, novel treatment strategies, and global cooperation are crucial for mitigating the impact of resistance and ensuring the effective management of Campylobacter-related infections.
Antimicrobial Effects of Metal Coatings or Physical, Chemical Modifications of Titanium Dental Implant Surfaces for Prevention of Peri-Implantitis: A Systematic Review of In Vivo Studies
Authors: Gkioka Maria , Rausch-Fan Xiaohui
Published: September 2024

Introduction: Peri-implantitis poses a significant challenge for implant dentistry due to its association with bacterial colonization on implant surfaces and the complexity of its management. This systematic review aims to assess evidence from in vivo studies regarding the antimicrobial efficacy of titanium (Ti) dental implant surfaces following physical/chemical modifications or the application of various metal element coatings in preventing bacterial growth associated with peri-implantitis. Materials and Methods: A literature review was conducted across four scientific databases (PubMed, Embase, Scopus, Web of Science), encompassing in vivo studies published between 2013 and 2024, and 18 reports were included in the systematic review. Results: The findings suggest that titanium dental implant surfaces, following physical/chemical modifications and metal element coatings, exhibit antimicrobial effects against bacteria associated with peri-implantitis in humans and various animal models. Conclusions: The reviewed studies indicated a reduction in bacterial colonization, diminished biofilm formation, and decreased signs of inflammation in the peri-implant tissues, which provides evidence that physical/chemical alterations on titanium dental implant surfaces or metal element coatings, like silver (Ag), zinc (Zn), magnesium (Mg), and copper (Cu), demonstrate antimicrobial properties in in vivo studies. However, caution is warranted when translating findings to clinical practice due to methodological disparities and high bias risks. Further larger-scale clinical trials are imperative to assess their long-term efficacy and validate their clinical applicability.
Redox Homeostasis and Molecular Biomarkers in Precision Therapy for Cardiovascular Diseases
Authors: Drăgoi Cristina Manuela , Diaconu Camelia Cristina , Nicolae Alina Crenguța , Dumitrescu Ion-Bogdan
Published: September 2024

Precision medicine is envisioned as the future of cardiovascular healthcare, offering a more tailored and effective method for managing cardiovascular diseases compared to the traditional one-size-fits-all approaches. The complex role of oxidative stress in chronic diseases within the framework of precision medicine was carefully explored, delving into the cellular redox status and its critical involvement in the pathophysiological complexity of cardiovascular diseases (CVDs). The review outlines the mechanisms of reactive oxygen species generation and the function of antioxidants in maintaining redox balance. It emphasizes the elevated reactive oxygen species concentrations observed in heart failure and their detrimental impact on cardiovascular health. Various sources of ROS within the cardiovascular system are examined, including mitochondrial dysfunction, which contributes to oxidative stress and mitochondrial DNA degradation. The article also addresses oxidative stress’s role in myocardial remodeling, a process pivotal to the progression of heart diseases. By integrating these aspects, the review underscores the importance of redox homeostasis and identifies molecular biomarkers that can enhance precision therapy for CVDs. The insights provided aim to pave the way for targeted therapeutic strategies that mitigate oxidative stress, thereby improving patient outcomes in cardiovascular medicine.
Oxidative Stress in Polycystic Ovary Syndrome: Impact of Combined Oral Contraceptives
Authors: Santander Nicolás , Figueroa Esteban G. , González-Candia Alejandro , Maliqueo Manuel , Echiburú Bárbara , Crisosto Nicolás , Salas-Pérez Francisca
Published: September 2024

Polycystic Ovary Syndrome (PCOS) is a complex hormonal disorder that is associated with heightened metabolic risks. While oxidative stress (OS) is known to play a role in PCOS, the precise nature of the relationship between PCOS and increased OS remains not entirely understood. Combined oral contraceptives (COCs) are the first-line treatment to regulate menstrual cycles and androgen levels, but their impact on oxidative stress requires further study. We conducted a transcriptomic analysis using RNAseq and assessed the levels of various oxidative stress (OS) markers in serum samples from women with PCOS and controls and whether they were using combined oral contraceptives (COCs), including enzymatic activities, FRAP, and 8-isoprostane (8-iso). A total of 359 genes were differentially expressed in women with PCOS compared to control women. Genes differentially expressed were enriched in functions related to inflammation and, interestingly, oxidative stress response. In controls, 8-iso levels were increased in women using COCs, whereas in women with PCOS, 8-iso levels were reduced in those using oral contraceptives (191.1 ± 97 vs. 26.4 ± 21 pg/mL, p: <0.0001). Correlation analyses showed a trend for a negative correlation between 8-iso and Ferriman score in women with PCOS consuming COCs (r = −0.86, p = 0.06) and a negative correlation between GSH and hyperandrogenism in women with PCOS (r = −0.89, p = 0.01). These results reveal the presence of lipid peroxidation in women with PCOS, which was modified by the use of COCs, providing new insights into the pathophysiology of PCOS in the Chilean population.