bluuebunny/crossref_metadata_2025 huggingface dataset:
Aminophylline targets miR-128-3p/Slc7a11 axis to attenuate neuronal ferroptosis after traumatic brain injury
Authors: Xu Yang , Liu Jinyuan , Sun Yuwen , Yang Qiuyun , Li Manrui , Liao Miao , Lv Meili , Liang Weibo , Chen Xiameng
Published: May 2023

Traumatic brain injury (TBI) is a significant global health issue due to its high morbidity and mortality, as well as its adverse outcomes. The resulting excessive healthcare and economic burden make it urgent to find effective drugs for TBI. Aminophylline (AMP), a classical drug widely used in the treatment of cardiovascular diseases and bronchiectasis, has recently been found to play an important role in protecting neurons in hypoxia brain injury by a variety of mechanisms. However, its therapeutic effect on TBI remains unknown. To this end, the present study aims to explore whether and how AMP improves TBI prognosis, as well as the underlying mechanisms. We found that AMP administration ameliorated neuronal ferroptosis in the cortex, improved cognitive impairment and motor dysfunction in mice following controlled cortical impact (CCI). microRNA (miRNA) profiling and related validation experiments revealed that miR-128-3p expression significantly decreased at 3h and 6h after CCI, and this change was further enhanced after AMP administration. Subsequent gain of function studies demonstrated that over-expressing miR-128-3p can aggravate neuronal ferroptosis by targeting recombinant solute carrier family 7, member 11 (Slc7a11), through which AMP exerted its therapeutic role in attenuating the cognitive impairment and motor dysfunction in mice following CCI. Overall, this study reports for the first time that AMP protects against ferroptotic neuronal death in CCI mice through miR-128-3p / Slc7a11 axis, which could be a potential treatment for TBI patients.
Groundwater level modeling using multi-objective optimization hybridizing artificial intelligence
Authors: Banadkooki Fatemeh Barzegari , Haghighi Ali Torabi
Published: May 2023

Estimating groundwater level (GWL) fluctuations is essential for integrated water resource management in arid and semi-arid regions. This study promotes the multi-layer perceptron (MLP) learning process using hybrid evolutionary algorithms. This hybrid metaheuristic algorithm was applied to overcome MLP difficulties in the learning process, including its low conversions and local minimum. Also, the hybrid model benefits from the advantages of two objective function procedures in finding MLP parameters that result in a robust model regardless of over and under-estimating problems. These algorithms include none dominated sorting genetic algorithm (NSGA II) and multi-objective particle swarm optimisation (MOPSO) in different patterns, including MLP–NSGA-II, MLP–MOPSO, MLP-MOPSO–NSGA-II, and MLP-2NSGA-II–MOPSO. Temperature, precipitation and GWL datasets were used in various combinations and delays as model input candidates. Finally, the best model inputs were selected using the correlation coefficient (R2). Input parameters include temperature and precipitation delays of 3, 6, and 9 months and GWL delays of 1 to 12 months. In the next step, the performance of the different combinations of MLP and hybrid evolutionary algorithms was evaluated using The root mean square error (RMSE), correlation coefficient (R), and mean absolute error (MAE) indices. The outcomes of these evaluations revealed that the MLP-2NSGA-II-MOPSO model, with an RMSE=0.073, R=0.98, and MAE=0.059, outperforms other models in estimating GWL fluctuations. The selected model benefits from the advantages of both MOPSO and NSGA-II regarding accuracy and speed. The results also indicated the superiority of multi-objective optimization algorithms in promoting MLP performance.
Molecular Epidemiological and Genetic Characterization of Pseudorabies Virus in Guangxi, China
Authors: Huang Xiangmei , Qin Shuying , Wang Xuying , Xu Lishi , Zhao Shuo , Ren Tongwei , Ouyang Kang , Chen Ying , Wei Zuzhang , Qin Yifeng , Huang Weijian
Published: May 2023

Pseudorabies virus (PRV) is an important pathogen that can cause harm to the pig population. However, there have been a number of large-scale outbreaks of pseudorabies on Chinese farms where animals have been previously vaccinated with the Bartha-K61 vaccine since 2011. In order to understand the epidemiological trend and genetic variations of PRV in Guangxi province, China, 819 tissue samples were collected from swine farms suspected of PRV infection from 2013–2019. In this study, these were tested for infectious wild strains of PRV. The results showed a positive rate of PRV in Guangxi province of 28.21% (231/819). 36 wild-type PRV strains were successfully isolated from the PRV-positive tissue samples, and a genetic evolutionary analysis was performed based on the gB, gC, gD, gE and TK genes. 30 of the PRV strains were clustered with the Chinese variant strains, HeN1-China-2012 and HLJ8-China-2013. In addition, 5 PRV strains were genetically related to the Chinese classical strains, and one isolate was a recombinant strain of the PRV variant and the vaccine strain, Bartha-K61. Amino acid sequence analysis showed that all 36 PRV strains had characteristic variant sites in the amino acid sequences of the gB, gC, gD and gE proteins. Pathogenicity analysis showed that, compared to the PRV classical strains, the PRV variant strains had a stronger pathogenicity in mice together with a lower LD50. Taken together, our results showed that wild-type PRV infections are common in pig farms in the Guangxi province of China, and that the dominant prevalent strains were those of the PRV variants. The PRV variant strains also had a stronger pathogenicity in mice. Our data will provide a useful reference for understanding the prevalence and genetic evolution of PRV in China.
Evaluation of nurses' knowledge and performance regarding preparation and injection of intravenous drugs in pediatric wards in Iran
Authors: Shahzeydi Amir , kallhor Faramarz , Khaksar Sajjad , Sabzghabaee Ali Mohammad , Joonbakhsh Fatemeh , Ajoodaniyan Najmeh
Published: May 2023

Background A error in intravenous injection in pediatric wards can cause irreparable injuries. This study aimed to determine the level of knowledge and performance of nurses in terms of preparation and injection of intravenous drugs in pediatric wards of hospitals affiliated to Isfahan University of Medical Sciences.Methods This cross-sectional study was conducted in 2022 on 156 nurses working in pediatric wards. The data was collected with demographic information questionnaire and the knowledge and performance of the participants were determined using a researcher-made questionnaire, including the five rights of medication administration (preparation and injection, medication error, drug side effects, family empowerment, and documentation) using self-reporting and observation methods. Formal and content validity was calculated using the opinions of 10 experts and Cronbach's alpha with 40 samples.Results The mean and standard deviation of total nurses' knowledge and performance scores were 58.31 + 10.1 and 66.1 + 14.4, respectively. Moreover, the mean and standard deviation of nurses' knowledge scores were 63.55 + 14.3 for documentation, 46.1 + 7.9 for preparation and injection, 73.9 + 12.3 for drug side effects, 58.4 + 10.2 for medication error, and 69.4 + 9.4 for family empowerment. Besides, the mean performance was 69.1 + 17.6 for documentation, 61.3 ± 9.9 for preparation and injection, 78.21 + 12 for drug side effects, 58.6 + 15 for medication error, and 65.4 + 17.7 for family empowerment.Conclusion The results showed that the mean knowledge and pharmacological performance of nurses working in pediatric wards in different areas of the principles of medicine were not at the desired level, and this can affect children adversely.
Influencing Factors of Post-traumatic Stress Disorder among Operating Room Nurses in Large Comprehensive Hospitals
Authors: Ge Yu-Xin , Sun Xin-Xing , Lin Chen , Yu Li , Zhang Jun
Published: May 2023

This is an investigation of the current situation of post-traumatic stress disorder (PTSD) among operating room nurses in large comprehensive hospitals and provide a scientific basis for managers to formulate effective intervention measures. In total, 266 operating room nurses were randomly selected from 6 large comprehensive hospitals in Zhejiang Province via a multi-stage random sampling method. The self-designed general information questionnaire and Chinese version of the PTSD self-rating scale (PCL-C) were used for the investigation. According to the PCL-C scores, the nurses were divided into two groups: ≥ 41 was divided into a PTSD group and < 41 was divided into a non-PTSD group. A single factor comparison was performed on the general data line and then a multivariate logistic analysis was conducted. The total score of the PCL-C was 45.41 ± 9.46 (95%CI 44.27–46.55), including an avoidance numbness symptom score of 19.13 ± 4.65 (95%CI 18.57–19.69), a re-experience symptom score of 12.82 ± 3.37 (95%CI 12.41–13.23), and a high vigilance symptom score of 13.46 ± 3.52 (95%CI 13.03–13.88). A univariate and multivariate logistic analysis showed there were significant differences in age, marital status, working years, professional titles, positions and shift arrangements between the two groups (P < 0.05). The PTSD incidence among operating room nurses in large comprehensive hospitals is relatively high. Factors such as age, marital status, working years, professional title, position, and shift scheduling significantly impacted their PTSD incidence. Therefore, managers can formulate effective intervention measures based on these results to reduce the incidence of PTSD among operating room nurses.
Clinical application of modified hip joint lateral position in femoral neck fracture
Authors: Liu Haitian , Gao Enpeng , Cao Lei , Zheng Yongli , Wen JinXu , Kong Lingde , Wu Wenjuan , Zhang Zuzhuo , Zhong Zhiwei
Published: May 2023

Background
To show the femoral neck better in hip lateral view of X-ray, we design a modified hip lateral view, and then investigate the value in femoral neck fractures.
Methods
CT images of 10 normal hip joints for 3D reconstruction were selected, the Mimics Medical 21.0 were used, and rotating the proximal femur was to find the most suitable angle for showing the femoral neck well, designed the modified lateral view according to this angle. We collected 25 femoral neck fracture and 25 healthy cases for observation and control group. And two groups were all taken hip anteroposterior, cross-table lateral and modified lateral view, which were analyzed by two radiologists to score the anatomical structures of the articular surface, femoral head, subfemoral head region, femoral neck, basal region and intertrochanteric region. Friedman Test was used to analyze the score of femoral neck at different angles. T test and Wilcoxon signed ranks test were to compare inter-groups.
Results
The modified lateral view was designed as follows: the subjects were supine, with the sagittal axis biased toward the healthy side at an angle of approximately 20° to the long axis of the examination table, the hip joint flexed at 45°, the lower extremity abducted at 40°, the centerline inclined 45° toward the head, and the centerline aligned with the center of the groin. The modified lateral view showed the femoral head, subfemoral head region and femoral neck more clearly than the lateral cross-table view, but the cross-table lateral showed the femoral neck basal and intertochanteric region better. In addition, the time of taking the modified lateral view was significantly less than the cross-table lateral view (healthy group: 0.752min ± 0.161 vs 0.596min ± 0.151, P < 0.001; fracture group: 1.128min ± 0.404 vs 0.924min ± 0.395, P < 0.001).
Conclusions
The modified lateral view can obtain a standard sagittal image of femoral neck, which can show the dislocation and angulation of the sagittal femoral neck fracture clearly, and improve the accuracy of diagnosis. And it is more convenient and easier for patients to cooperate, which is worthy promoting and applying in clinical work.
Observation of photonic Peierls transition for manipulating electromagnetic waves in metallic periodic structures
Authors: Wu Chia Ho , Liu Chengyang , Lin Xianqing , Wang Wei , Guo Yi Chun , Wang Zhuoyua , Ye Guoqiang , He Fang , Ni Donghua , Wang Xiaolong , Shen Linfang , Shen Jianqi
Published: May 2023

Peierls transition that modifies electronic band structure has attracted intensive attention in solid state physics. In the present work, a photonic analog of Peierls transition has been observed in a 1-D triangular metal diaphragm array, where the photonic bandgap structures have been designed at will by adjusting periodically the metal diaphragm positions. It is shown by the numerical analysis that the transmission and radiation effect of the present periodic metal structure designed through the Peierls transition rule exhibits the behavior significantly different from the original periodic structure with each unit cell containing a metal diaphragm. The near- and far-field measurement results are in good agreement with our theoretical simulation. The present effect of photonic Peierls transition can serve as a working mechanism for designing new types of guided wave devices. It can be seen that the photonic Peierls transition would be one of the simplest ways for modifying the transport characteristics of periodic structures.
Visual Attention and Memory in Professional Traders: A Behavioural Study
Authors: Bossi Francesco , Malizia Andrea P. , D’Arcangelo Sonia , Maggi Francesca , Lattanzi Nicola , Ricciardi Emiliano
Published: May 2023

Professional traders need to process a large amount of visual information in their daily activity to judge how risky it is to trade specific investment products. Despite some studies investigating the effects of display clutter on traders, visual attention and memory were never investigated in controlled experimental tasks in this population. Following a preliminary study with 30 participants, visual selective attention and visual working memory were measured and compared between two groups of 15 traders and 15 non-traders (salespeople, acting as a control group) from a large-scale banking group in three experimental tasks measuring selective attention in complex visual contexts, simulating display clutter situations (Visual search), cognitive interference (Stroop task), and a delayed recall visual working memory task. In the Visual search task, traders displayed a better Inverse Efficiency Score (IES) than non-traders for small display sets, while their performance overlapped for large sets. In the Stroop task, traders showed better IES than non-traders but were nevertheless affected by cognitive interference. The memory task highlighted no significant differences between the groups. Therefore, this study found an advantage in traders’ attention when processing visual information in small sets with no retention. This result could influence trading activity - determining an immediate use of relevant visual information in decision making - and traders’ display layout organization.
Effects of micro-algae oil replacement on growth, omega-3 deposition and gut microbiome composition of Nile tilapia (Oreochromis niloticus)
Authors: Trevi S. , Webster T. M. Uren , Consuegra S. , de Leaniz C. Garcia
Published: May 2023

Microalgae offer a sustainable source of omega-3 fatty acids that can replace fish oil in aquafeeds, but the nutritional benefits are not always clear, particularly when microalgae are used as complete oil replacements in starter feeds. We compared the survival, growth, omega-3 deposition and composition of the gut microbiota of Nile tilapia fed with aquafeeds that differed in dietary oil, from plant, fish and microalgae (Schizochytrium) origins. Survival was not affected by diet, but fish fed a diet where the entire oil component (5%) was replaced by microalgae oil grew twice as fast than fish fed plant oil or a mixture of plant and fish oil. Dietary omega-3 content was strongly correlated with omega-3 deposition in the fish fillet. Complete replacement of fish oil by plant oil caused a significant increase in the abundance of Aeromonadaceae which is often associated with an inflammatory response in the fish gut. In contrast, when fish oil was replaced by microalgae oil an increase in Peptostreptococcaceae and Mycobacteriaceae was observed. Our study indicates that Schizochytrium oil can be used to improve the growth of Nile tilapia and increase its omega-3 content without any of the detrimental effects on the gut microbiome typically associated with some plant oil replacements.
Impact of Elobixibat on Liver Tumors, Microbiome and Bile Acid Levels in a Mouse Model of Nonalcoholic Steatohepatitis
Authors: Sugiyama Yoshiaki , Yamamoto Kenta , Honda Takashi , Kato Asuka , Muto Hisanori , Yokoyama Shinya , Ito Takanori , Imai Norihiro , Ishizu Yoji , Nakamura Masanao , Asano Tomomi , Zaitsu Kei , Ishigami Masatoshi , Fujishiro Mitsuhiro , Kawashima Hiroki
Published: May 2023

Background
Elevated bile acid levels have been associated with liver tumors in fatty liver. Ileal bile acid transporter inhibitors may inhibit bile acid absorption in the distal ileum and increase bile acid levels in the colon, potentially decreasing serum and hepatic bile acids. This study investigated the impact of these factors on tumors.
Methods
C57BL/6J mice received a one-time intraperitoneal injection of 25 mg/kg of diethylnitrosamine. They were fed a choline-deficient high-fat diet for 20 weeks starting from 8 weeks of age, with or without elobixibat (EA Pharma, Tokyo, Japan).
Results
Both groups showed liver fat accumulation and fibrosis, with no significant differences between the two groups. However, mice treated with elobixibat showed fewer liver tumors. The total serum bile acid levels, including free-/tauro-/gly- conjugated bile acids and tauro-α/β-muricholic acids in the liver, were noticeably reduced with elobixibat. The proportion of gram-positive bacteria in feces was significantly lower in the group treated with elobixibat (5.4%) than in the group without elobixibat (33.7%).
Conclusion
Elobixibat suppressed tumor growth by inhibiting bile acid reabsorption and decreasing total bile acid and primary bile acid levels in the serum and liver. Additionally, the presence of bile acids in the colon may have led to a significant reduction in the proportion of gram-positive bacteria, potentially resulting in decreased secondary bile acid synthesis.
A cell-free strategy for profiling of intracellular antibiotic sensitivity and resistance
Authors: Chengan Kameshwari , Hind Charlotte , Nagappa Lakshmeesha , Wand Matthew , Sutton John , Hanson Tanith , Smales Mark , Tsaousis Ruben , Escolano Ruben Martin , Bengoechea Jose , Moore Simon
Published: May 2023

Antimicrobial resistance (AMR) is a pandemic spread across multiple priority infectious disease threats. Herein, we present a Klebsiella pneumoniae cell-free gene expression (CFE) system for the rapid profiling of intracellular antibiotic sensitivity and resistance. Specifically, this cell-free approach provides multiple antibiotic targets, which we exploit to identify which antibiotics are potent inside the cell but have limited transport. We also derive CFE systems from a selection of laboratory-adapted K. pneumoniae AMR variants, and as an exemplary case, show that a single RNA polymerase beta subunit variant H526L confers a 58-fold increase in CFE resistance to rifampicin. Overall, our cell-free model provides a safe (i.e., non-living) and rapid platform suitable for studying AMR variants in a Containment Level 1 laboratory. The approach is generalisable to laboratory and clinical strains and provides a new experimental tool that will significantly advance the understanding of antibiotic transport, AMR and provides a new infectious disease model.
SEMORE: SEgmentation and MORphological fingErprinting by machine learning automates super-resolution data analysis
Authors: Bender Steen , Dreisler Marcus , Zhang Min , Kaestel-Hansen Jacob , Hatzakis Nikos
Published: May 2023

The morphology of protein assemblies impacts their behavior and contributes to beneficial and aberrant cellular responses. While single-molecule localization microscopy provides the required spatial resolution to investigate these assemblies, the lack of universal robust analytical tools to extract and quantify underlying structures limits this powerful technique. Here we present SEMORE, a semi-automatic machine learning framework for universal, system and input-dependent, analysis of super-resolution data. SEMORE implements a multi-layered density-based clustering module to dissect biological assemblies and a morphology fingerprinting module for quantification by multiple geometric and kinetics-based descriptors. We demonstrate SEMORE on simulations and diverse raw super-resolution data; time-resolved insulin aggregates and imaging of nuclear pore complexes. SEMORE extracts and quantifies all protein assemblies enabling classification of heterogeneous insulin aggregation pathways and NPC geometry in minutes. SEMORE is a general analysis platform for super-resolution data, and being the first time-aware framework can also support the rise of 4D super-resolution data.
Exploration of the Molecular Mechanism of Intercellular Communication in Paediatric Neuroblastoma by Single-Cell Sequencing
Authors: Chu Jing
Published: May 2023

Background
Neuroblastoma (NB) is an embryonic tumour originating from the sympathetic nervous system that most often occurs in infants and children under 2 years of age. Moreover, it is the most common extracranial solid tumour in children. Increasing research suggests that intercellular communication within the tumour microenvironment is closely related to tumour development.
Methods
This study aimed to construct a prognosis-related intercellular communication-associated genes (ICAGs) model by single-cell sequencing and transcriptome sequencing to predict the prognosis of patients with NB for precise management. Single-cell data from patients with NB were downloaded from the Gene Expression Omnibus database for comprehensive analysis. Moreover, prognosis-related genes were screened in the TARGET database based on epithelial cell marker genes through a combination of Cox regression and Lasso regression analyses, using GSE62564 and GSE85047 for external validation. Patients’ risk scores were calculated, followed by immune infiltration analysis, drug sensitivity analysis, and enrichment analysis of risk scores, which were conducted for the prognostic model.
Results
We used the feature selection algorithm with Lasso regression to screen for characteristic genes in NB and developed a 21-gene prognostic model. The risk scores were highly correlated with multiple immune cells and common anti-tumour drugs. In addition, the risk score was identified as an independent prognostic factor for NB.
Conclusions
In this study, we constructed and validated a prognostic signature based on epithelial marker genes, which may provide useful insights into the development and prognosis of NB.
RNF213 inhibits migration via mediating KRT16 ubiquitination in lung adenocarcinoma cell
Authors: Pei Xiaofeng , Liu Xiaoli , Zeng Huanwen , Zeng Fangfang , Wei Chunren , Zhang Zhaoyuan , Chen Nan , Zhang Hongyu
Published: May 2023

Background Non-small cell lung cancer (NSCLC) is one of the most common malignant tumors, and lung adenocarcinoma (LUAD) accounts for 40% of NSCLC. Ring finger protein 213(RNF213) has been shown to inhibit the progression of many different cancers including glioblastoma and breast cancer. However, the role of RNF213 in LUAD has not been reported. The aim of this study was to investigate the effect of RNF213 on the progression of LUAD.Methods The expression of RNF213 in LUAD tissues was analyzed by western blot, TCGA (The Cancer Genome Atlas) and GTEx (Genotype_Tissue Expression Project) databases. Kaplan-Meier Plotter database was used to predict the clinical significance of RNF213 in LUAD. We determined the role of RNF213 in LUAD cell lines through CCK-8 assay, migration and invasion assay. The relationship of RNF213 and KRT16 were demonstrated via co-immunoprecipitation, ubiquitination, protein degradation assay and immunoblotting. We next confirmed the oncogenetic role of KRT16 using migration and rescue assay. The clinical roles of RNF213 and KRT16 were explored by immunohistochemical staining assay (IHC) and Kaplan-Meier survival analysis.Results Our data manifested that RNF213 expression was reduced in LUAD, thereby affecting the prognosis of LUAD. The molecular study revealed that RNF213 functioned by promoting KRT16 ubiquitination. IHC data analysis showed that KRT16 was negatively correlated with RNF213 protein expression, and downregulation of RNF213 was associated with poor overall survival.Conclusion RNF213 is a potential tumor suppressor that inhibits migration of LUAD cell by mediating KRT16 ubiquitination.
Validation of the Italian Version of the Peyronie’s Disease Questionnaire (PDQ)
Authors: Grazia Massimo Di , Ongaro Luca , Rizzo Michele , Cocci Andrea , Verze Paolo , Zucchi Alessandro , Cai Tommaso , Salame' Leonardo , Garaffa Giulio , Trombetta Carlo , Liguori Giovanni , Traunero Fabio
Published: May 2023

Background/objectives: Peyronie's disease (PD) is a connective tissue disorder characterized by the formation of fibrous plaques in the tunica albuginea of the penis, resulting in pain, deformity, and erectile dysfunction. The Peyronie's Disease Questionnaire (PDQ) assesses the severity of symptoms, including pain, penile curvature, and erectile dysfunction, as well as the impact on sexual function, emotional well-being, and overall quality of life. Previous studies validated the PDQ US version and confirmed its test–retest reliability and responsiveness. The aim is to translate and validate the Italian version of the PDQ to be used in clinical practice and in PD research studies in Italy.Methods A non-interventional, observational study with 80 PD patients was conducted in 6 Italian high-volume centers, completing the PDQ in two different study visits. Patients received no type of treatment.Results PDQ-I has excellent test-retest reliability in the Italian population (ICC were between 0.86 and 0.92). Moreover, PDQ-I provides strong internal consistency, with all three scale scores exceeding the objective Cronbach's alpha coefficient of ≥ 0.70Conclusions The translation and validation of the PDQ for the Italian population provides a valid, useful, and reliable tool to clinically evaluate the quality of life of PD patients and to improve studies on the subject.
Clinical importance of TYMS and ENOSF1 genetic variants and mRNA expression levels in response to chemotherapy in Iranian gastric cancer patients
Authors: Arjmandi Khadijeh , Salahshourifar Iman , Irani Shiva , Ameli Fereshteh , Esfandbod Mohsen
Published: May 2023

Background Genetic variants are powerful tools for predicting the effectiveness of chemotherapy drugs. Thymidylate synthase (TS) is a crucial enzyme in gastric cancer patients given neoadjuvant chemotherapy based on 5-Fluorouracil (5-FU). The enolase superfamily member 1 (ENSOF1) variant affects TS gene (TYMS) expression and thus may affect chemoresistance in gastric cancer. Hence, this study aims to examine the 28 bp VNTR variant on the 5'UTR of the TYMS gene and rs2612091 and rs2741171 variants of the ENOSF1 and the possible effects of their alleles on chemo-resistance, survival, and gene expression among gastric cancer cases.Methods and Results Genetic analysis was performed on 100 matched FFPE blocks for normal tissues of gastric cancer cases. All patients were treated with neoadjuvant chemotherapy based on 5-FU. RNA was obtained from the tumor and matched normal tissues. The 28 bp VNTR, rs2612091, and 2741171 variants were genotyped, and the gene expression was analyzed using Real-Time PCR. A significant association was observed between genotypes of VNTR and the treatment (p = 0.032). Cases with the 2R3R genotype had a better response to the treatment. The highest and lowest average survival times of patients were observed in the 3R3R and 2R2R genotypes, respectively (p = 0.003). The 3R3R genotype was associated with higher TYMS expression (P < 0.001). There was a significant relationship between genotypes rs2612091 and the treatment (p = 0.017).Conclusions This research indicated that genotyping and gene expression analysis of TYMS and ENOSF1 might be used to predict gastric cancer survival and responsiveness to 5-FU-based neoadjuvant treatment.
Facilitators and barriers to communication in rehabilitation services across healthcare levels: A qualitative case study in a Norwegian context
Authors: Skumsnes Randi , Thygesen Hilde , Groven Karen Synne
Published: May 2023

Background People experiencing functional decline following injury or illness often need multiple and combined interventions in their rehabilitation processes. This means that collaboration and coordination between the involved healthcare professionals are essential. Interprofessional collaboration has, to a large extent, been explored in previous research, which has indicated the importance of facilitators of communication. Despite efforts in both research and policy, challenges in practice continue, both in the collaboration across specialist and primary levels of healthcare and within primary healthcare. Context-bound research is needed to gain insight into the complexity of these communication challenges. In this study we explore facilitators and barriers to communication in the context of neurological rehabilitation from specialist care at hospital to municipality primary care in a region of Norway. We address the transfer and exchange of relevant patient information between involved healthcare professionals.Methods A qualitative case study design was used to explore the exchange of patient information in the neurological rehabilitation processes for four patients. The data collection included participant observations in communication situations and an exploration of the electronic patient records of the same four patients. Reflexive thematic analysis was used to code and analyse the data, and the theory of practice architecture was used to interpret and structure the findings.Results We found three main barriers to communication: lack of a common understanding and culture for rehabilitation, poor access to written information and unclear authority and responsibilities for sharing information. These barriers are intertwined and influence communication practices.Conclusion The chains of interventions in the rehabilitation process depend on extensive information exchange among many of the health professionals involved, making collaboration complex. The barriers to exchanging relevant patient information need to be acknowledged as well as how informal relations among the involved facilitate the flow of information. More extensive use of joint communication tools may contribute to handling the complexity and to reducing barriers to communication.
Establishment and validation of a prognostic signature for lung squamous cell carcinoma based on metabolism‐related genes
Authors: Wu Mengxi , Yan Jun , Guo Quanwei , Zhang Tao , Tan Jianfeng , Li Dongfang , Kuang Jun , Mo Yijun , Zhang Jianhua
Published: May 2023

Background
Lung squamous cell carcinoma (LUSC) is a main subgroup of non-small cell lung cancer that accounts for ~ approximately 80% of all lung cancer. Studies have demonstrated that tumor microenvironment is essential for the occurrence and development of LUSC. The present study identified the immune- and metabolic-related genes to construct a prognostic model for LUSC patients.
Methods
We downloaded RNA-sequence data and clinical information of LUSC patients from The Cancer Genome Atlas database. The differentially expressed metabolic-related genes (DMRGs) and the differentially expressed immune-related genes (DIRGs) between the control samples and LUSC patient samples were identified. Gene ontology analysis and Kyoto Encyclopedia of Genes and Genomes analysis were performed to explore the potential biological processes of harvested DMRGs and DIRGs. To develop a prognostic model for LUSC patients, we selected prognostic genes from DMRGs and DIRGs through the univariate and multivariate Cox regression. The prognostic value of the risk model was detected via the Kaplan-Meier curve and the area under the receiver operating characteristic curve analysis. Gene set enrichment analysis (GSEA) was operated to investigate the underlying latent pathways associated with the high-risk group. The infiltration of immune cells in the high- and low-risk groups were analyzed using the single-sample gene-set enrichment analysis.
Results
A total of 178 DMRGs were identified, which were mainly enriched in nucleoside phosphate biosynthetic process, carboxylic acid biosynthetic process, and cofactor metabolic process. Around 264 DIRGs were primarily associated with chemotaxis regulation, leukocyte migration, and positive regulation of cytokine production. After the univariate and multivariate Cox regression analyses, a prognostic risk model containing four prognostic genes was constructed, which performed better in prognosing LUSC patients via the ROC analysis. Moreover, the risk score was found to be an independent prognostic factor through the multivariate Cox regressions analysis. The GSEA suggested that the chemokine signaling pathway, cytokine-cytokine receptor interaction, and IL-17 signaling pathway were mainly enriched in the high-risk group. Finally, the risk score was mostly correlated with immune-related score and immune infiltration cells.
Conclusion
We constructed an immune- and metabolic-related gene signature that was quite reliable in predicting prognosis of LUSC patients.
Major lung resection for carcinoid tumor; Does it affect quality of life?
Authors: Abdeljalil Riad , Abdallah Farah A. , Obeid Zeinab , chaar Mohamad K. Abou , Harb Ahmad Khaled , Shannies Tariq Bassam , El-Edwan Ahed , Haddad Hussam , Ghraibeh Azza , Abu-Shanab Ahmad
Published: May 2023

Background:
Pulmonary carcinoid is rare with surgery remaining the gold standard treatment which may include radical lung resection as well as minimally invasive procedures and parenchymal sparing bronchial resection. However, resection may impact patients' daily activities due to decreased lung volume reserve and postoperative pain. Our study aims to compare the impact of different types of surgical resection on the post-operative quality of life with the application of a strict peri-operative pulmonary care program.
Methods:
Patients who underwent surgery for bronchopulmonary carcinoid tumors in a tertiary cancer center between August, 2017 and March, 2020 were included. For the qualitative and quantitative assessment of pain and quality of life, we utilized the Arabic version of Short-form McGill Pain Questionnaire and Activity of Daily Living (ADL) instrument respectively. Lung reserve was assessed before and after surgery. Statistical analysis conducted on SPSS version 24.
Results:
A total of 16 patients underwent different type of resection. The majority were males (n=10; 63%) with a mean age of 44 years (19-81). Most common clinical stage was stage I (n=12, 75%) with typical carcinoid features recorded in more than half of the cases (n=11, 69%). Almost all patients underwent surgical excision (n=15, 94%) with negative resection margin and no major post-operative complications. Bilobectomy was the most frequent procedure (n=6, 40%) and video-assisted thoracoscopic surgery (VATS) was utilized in 8 patients (50%). Expected changes were recorded in pre- and postoperative pulmonary function test. The majority of patients (n=15, 94%) were totally independent. Mild intermittent pain was found in 7 patients (44%) who scored an average intensity of 1.6 out of 10.
Conclusions:
Excellent long-term outcomes can be achieved following surgical resection of pulmonary carcinoid tumors with little to no effect on patients' lung function and quality of life in regard to performance status and post-operative pain when a good peri-operative pulmonary, physical rehabilitation, and pain management programs are adopted and strictly implemented.
Hydrogels with Tunable Mechanical Plasticity Regulate Endothelial
Cell Outgrowth in Vasculogenesis and Angiogenesis
Authors: Wei Zhao , Lei Meng , Wang YaoHui , Xie Yizhou , Xie Xueyong , Lan DongWei , Jia Yuanbo , Liu Jingyi , Ma Yufei , Chen Bo , Gerecht Sharon , Xu Feng
Published: May 2023

The endothelial cell (EC) outgrowth in both vasculogenesis and angiogenesis starts with remodeling surrounding matrix and proceeds with the crosstalk between cells for the multicelluar vasculature formation. Althought the mechanical plasticity of matrix has been recognized to regulate individual cell behaviors through plastic remodeling, it remains elusive how matrix plasticity impacts cell-to-cell interplay during EC outgrowth and the underlying molecular pathways. Here we developed a collagen-hyaluronic acid based hydrogel platform with tunable plasticity independent of stiffness, by using compositing strategy of dynamic and covalent crosslinks. We show that the increasing plasticity of the hydrogel networks facilitates the matrix remodeling by ECs, thus promoting the process of individual sprouting and branching during vasculogenesis. However, the lagest tubular lumens and the longest invading distance from EC spheoid unexpectedly appear in hydrogels with medium plasticity instead of the highest ones. We unravel that althought the high plasticity of the hydrogels promotes stable integrin cluster of ECs and recruitment of focal adhesion kinase (FAK) with an increase in cell contractility, while the overenhanced contractility downregulates the vascular endothelial calmodulin (VE-CAD) expression and destabilizes the adherens junctions between individual ECs in vasculogenesis (or stalk and tip ECs in angiogenesis). Our results, further validated with mathematical simulations and in vivo angiogenic tests, demonstrate that a balance of matrix plasticity facilitates both cell-matrix binding and cell-to-cell adherens, for promoting vascular assembly and invasion.
Automated cerebral hemorrhage volume calculation and stability detection using automated software
Authors: Sreekrish Anirudh , Venkatasubramanian Chitra , Heit Jeremy J
Published: May 2023

Introduction: The measurement of intracerebral hemorrhage (ICH) volume is important for management, particularly in evaluating expansion on subsequent imaging. However manual volumetric analysis is time-consuming, especially in busy hospital settings. We aimed to use automated Rapid Hyperdensity software to accurately measure ICH volume across repeated imaging.Methods We identified ICH cases, with repeat imaging conducted within 24 hours, from two randomized clinical trials where enrollment was not based on ICH volume. Scans were excluded if there was (1) severe CT artifacts, (2) prior neurosurgical procedures, (3) recent intravenous contrast, or (4) ICH < 1 ml. Manual ICH measurements were conducted by one neuroimaging expert using MIPAV software and compared to the performance of automated software.Results 127 patients were included with median baseline ICH volume manually measured at 18.18 cc (IQR: 7.31–35.71) compared to automated detection of 18.93 cc (IQR: 7.55, 37.88). The two modalities were highly correlated (r = 0.994, p < 0.001). On repeat imaging, the median absolute difference in ICH volume was 0.68cc (IQR: -0.60-4.87) compared to automated detection at 0.68cc (IQR: -0.45-4.63). These absolute differences were also highly correlated (r = 0.941, p < 0.001), with the ability of the automated software to detect ICH expansion with a Sensitivity of 94.12% and Specificity 97.27%.Conclusion In our proof-of-concept study, the automated software has high reliability in its ability to quickly determine IPH volume with high sensitivity and specificity and to detect expansion on subsequent imaging.
Multicenter integrative analysis identifies a multigene signature for lung adenocarcinoma prognosis via glycolysis metabolism discoordination
Authors: Zhang Nannan , Dong Wenlei , Li Junheng , Luo Yuting , Zhu Kunmei , Ren Jie , Li Luyang , Zhang Xinxin , Liu Yixin
Published: May 2023

Background
To explore reliable and reproducible prognostic signatures to aid in guiding clinical decision-making, the present study proposed an integrative analysis method to identify a function-derived gene signature for lung adenocarcinoma (LUAD) prognosis.
Methods
Total 1238 LUAD patients treated with curative resection alone were sourced from public datasets. Using three cohorts of 665 patients in the meta-discovery dataset, we first utilized an integrative analysis method to extract prognostic genes, and identified the essential prognostic genes from a function-derived perspective. Thereafter, we proposed pairwise comparison of single-sample gene set enrichment method to establish a multigene signature for LUAD prognosis based on the function-derived prognostic genes.
Results
Based on integrative analysis, we identified 14 metabolic-related prognostic genes involved in glycolysis metabolism, and established a function-derived signature consisting of these genes for LUAD prognosis (14GM-PS). The prognostic performance of the signature was rigorously validated in two multiple cross-platform independent datasets comprising 299 (log-rank P = 2.78E-06) and 274 (log-rank P = 0.0042) patients, respectively, with significantly different 5-year survival rate. Multivariate Cox analysis demonstrated that the function-derived signature was an independent prognostic factor for LUAD prognosis. Furthermore, the novel proposed nomogram significantly improved the prognostic performance (concordance index) of clinicopathological factors. The molecular and immune characteristics analysis showed that high-risk patients identified by 14GM-PS were characterized by higher hypoxia, proliferation and stemness scores, and lower immune score, providing evidence that could reflect transcriptomic characteristics that are strongly associated with clinical outcomes in the molecular mechanism.
Conclusion
This multicenter study illustrates the accuracy and stability of the function-derived signature for LUAD prognosis, and might become a promising genomic tool to guide individualized application and decision-making of LUAD in clinical practice, with further prospective validation in clinical trials.
Association between dietary folate and migraine in US adults: a cross-sectional study
Authors: Zhu Xiao-Feng , Mo Ye-Tong , Feng Yu-Xue , Liao Wan-Zhe , Wang Lan-Lan , Zheng Cheng , Guo Xu-Guang
Published: May 2023

Background Migraine is a highly prevalent disabling neurological disease that affects 1.04 billion people worldwide. Folate is an essential vitamin B for human neurological health. However, studies between dietary folate and migraines are limited.Methods Data from 9679 participants aged ≥ 20 were collected from the National Health and Nutrition Examination Survey (NHANES) between 1999 and 2004. Multivariable logistic regression models, smooth curve fittings, subgroup analyses, and threshold examination were used to check the association between dietary folate and migraines.Results A negative association was exhibited between dietary folate and the prevalence of migraines in the adjusted model. The association between dietary folate and migraines showed a U-shaped curve, with a cutoff point of 1209 mcg. In the stratified analysis, the correlation was subsistent in most subgroups, while no significant interaction effect was found between different subgroups (all P for interaction > 0.05).Conclusion High folate intake was significantly related to a lower odds ratio of migraines. In the future, more studies are needed to confirm our results, while necessary experiments are necessary to explore the exact mechanisms to benefit the treatment and prevention of migraine.
Sterile Insect Technique integration with mass trapping in management of the carob moth, Ectomyelois ceratoniae (Lep.: Pyralidae) in pomegranates
Authors: Soufbaf Mahmoud , Ghorbani Roshanak , Hayatolgheibi Seyed Mohammad Hosein
Published: May 2023

The carob moth, Ectomyelois ceratoniae (Lepidoptera: Pyralidae) is the most important insect pest of pomegranates in Iran. In this research, applicability of the Sterile Insect Technique (SIT) was assessed using three strategies including SIT, SIT + mass trapping (MT) by female sex pheromone, and MT in three isolated orchards in Iran during two consecutive years, 2018 and 2019. Mass rearing of the moths was done in clean rooms on an artificial diet under constant environmental conditions and 200 Gy was applied as the sterilizing dose of gamma irradiation at pupal stage. Insect pest damage was declined in both 2018 and 2019 in all treatments significantly. As a result of SIT in 2018 and 2019, damage of the pest reduced 11.6 and 11.8 times compared to control orchard, respectively. Also, as a result of applying SIT + MT in 2018 and 2019, we observed 2.26 and 2.79 times decrease in the damage relative to the control orchard, respectively. In this way, SIT has been 5.14 and 4.23 times more successful than SIT + MT in 2018 and 2019, respectively. Due to lack of synthetic sex pheromone source, MT treatment was replaced with biological control treatment by Trichogramma embryophagum in 2019 and SIT was more successful than the SIT + parasitoid considerably.
High incidence of metabolic diseases and sedentary lifestyle underline the need for regular counseling in LTFU for CCS- results from a monocentric prospective study
Authors: Richter Franziska , Kronziel Lea Louisa , König Inke Regina , Langer Thorsten , Gebauer Judith
Published: May 2023

Background
Many childhood cancer survivors (CCS) develop treatment-related late effects years after the end of treatment, including increased risk of obesity, metabolic syndrome and related diseases. A healthy lifestyle can reduce the risk of associated comorbidities. Lifestyle counseling during regular long term follow up (LTFU) care could benefit these at-risk CCS.
Methods
Lifestyle counseling was implemented within LTFU care over the study period of one year. Metabolic markers and activity levels were prospectively evaluated as screening parameters for different risk groups. Perspectives of CCS, Physician and sports scientist were compared to identify those in need of counseling. Additionally, a follow-up appointment after one month was performed.
Results
During the counseling offer, 155 CCS (65% female, 35% male) aged between 18 and 63 years (median:30 years) were contacted. Among them, 112 CCS (72%) had an indication for lifestyle counseling based on assessments from physicians, sports scientists, or CCS themselves. 45% were affected by metabolic disorders, 25% met the criteria through BMI and 46% did not meet the recommended activity levels. A total of 120 CCS (77%) received lifestyle counseling, with those who underwent intensive cancer treatment showing the highest need. During the follow-up appointment, CCS indicated high adherence to recommendations and successful implementation into their daily lives.
Conclusion
Metabolic disorders and reduced physical activity impact around two-thirds (68%) of CCS. Nearly all survivors (97%) find the provision of lifestyle counseling during regular LTFU beneficial. Incorporating specialized healthcare professionals into survivorship care enhances the multidisciplinary approach and can aid in promoting a healthy lifestyle, potentially reducing long-term morbidity among CCS.