bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of Multistage Solution Aging Heat Treatment on Mechanical Properties and Precipitated Phase Characteristics of High-Strength Toughened 7055 Alloy
Authors: Li Qilun , Zhang Xiaobo , Guo Ce , Qiao Jisen
Published: April 2024

In this paper, a one-step hot extrusion dual-stage solution treatment method is employed to fabricate high-strength and tough T-shaped complex cross-section 7055 (Al-Zn-Mg-Cu-Zr) alloy profiles, and a detailed investigation is conducted on the microstructure and mechanical properties. The results indicate that the comprehensive mechanical properties of the 7055 aluminum extruded alloy using the two-stage solution aging treatment are excellent. This is particularly evident in the balance between strength and ductility, where outstanding strength is accompanied by a plasticity that is maintained at 13.2%. During the extrusion process, the deformation textures are mainly composed of brass and copper, forming a 15.1% recrystallization texture Cube. In addition, the equilibrium phase η(MgZn2) precipitated in the grain is the main strengthening phase, and there are large discontinuous grain boundary precipitates at the grain boundary, which hinders the grain boundary dislocation movement and has great influence on the mechanical properties of alloy materials.
A Polarization-Insensitive, Vanadium Dioxide-Based Dynamically Tunable Multiband Terahertz Metamaterial Absorber
Authors: Raza Mohsin , Li Xiaoman , Mao Chenlu , Liu Fenghua , He Hongbo , Wu Weiping
Published: April 2024

A tunable multiband terahertz metamaterial absorber, based on vanadium dioxide (VO2), is demonstrated. The absorber comprises a three-layer metal–insulator–metal (MIM) configuration with a split ring and slots of VO2 on the uppermost layer, a middle dielectric substrate based on silicon dioxide (SiO2), and a gold reflector on the back. The simulation results indicate that, when VO2 is in the metallic state, the proposed metamaterial exhibits nearly perfect absorption at six distinct frequencies. The design achieves an average absorption of 98.2%. The absorptivity of the metamaterial can be dynamically tuned from 4% to 100% by varying the temperature-controlled conductivity of VO2. The proposed metamaterial absorber exhibits the advantages of polarization insensitivity and maintains its absorption over 80% under different incident angle conditions. The underlying physical mechanism of absorption is explained through impedance matching theory, interference theory, and the distribution of electric fields. The ability to achieve multiband absorption with tunable characteristics makes the proposed absorber a promising candidate for applications in terahertz sensing, imaging, communication, and detection. The polarization insensitivity further enhances its practicality in various scenarios, allowing for versatile and reliable performance in terahertz systems.
Assessing Microplastics and Nanoparticles in the Surface Seawater of Venice Lagoon—Part I: Methodology of Research
Authors: Cecchi Teresa , Poletto Davide , Berbecaru Andrei Constantin , Cârstea Elfrida Mihaela , Râpă Maria
Published: April 2024

Microplastics (MPs) and nanoplastics (NPs) both represent significant concerns in environmental sciences. This paper aims to develop a convenient and efficient methodology for the detection and measurement of MPs and nanoparticles from surface seawater and to apply it to the water samples collected from the UNESCO site of Venice and its lagoon, more precisely in the Venice-Lido Port Inlet, Grand Canal under Rialto Bridge, and Saint Marc basin. In this study, MPs were analyzed through optical microscopy for their relative abundance and characterized based on their color, shape, and size classes, while the concentration and the mean of nanoparticles were estimated via the Nanoparticle Tracking Analysis technique. Bulk seawater sampling, combined with filtration through a cascade of stainless-steel sieves and subsequent digestion, facilitates the detection of MPs of relatively small sizes (size classes distribution: >1 mm, 1000–250 μm, 250–125 μm, 125–90 μm, and 90–32 μm), similar to the size of MPs ingested by marine invertebrates and fishes. A protocol for minimizing interference from non-plastic nanoparticles through evaporation, digestion, and filtration processes was proposed to enrich the sample for NPs. The findings contribute to the understanding of the extent and characteristics of MPs and nanoparticle pollution in the Venice Lagoon seawater, highlighting the potential environmental risks associated with these pollutants and the need for coordinated approaches to mitigate them. This article is based on scientific research carried out within the framework of the H2020 In-No-Plastic—Innovative approaches towards prevention, removal and reuse of marine plastic litter project (G.A. ID no. 101000612).
Safe Hybrid-Action Reinforcement Learning-Based Decision and Control for Discretionary Lane Change
Authors: Xu Ruichen , Xu Jinming , Liu Xiao , Lin Yuan
Published: April 2024

Autonomous lane-change, a key feature of advanced driver-assistance systems, can enhance traffic efficiency and reduce the incidence of accidents. However, safe driving of autonomous vehicles remains challenging in complex environments. How to perform safe and appropriate lane change is a popular topic of research in the field of autonomous driving. Currently, few papers consider the safety of reinforcement learning in discretionary lane-change scenarios. We introduce safe hybrid-action reinforcement learning into discretionary lane change for the first time and propose the Parameterized Soft Actor–Critic with PID Lagrangian (PASAC-PIDLag) algorithm. Furthermore, we conduct a comparative analysis with Parameterized Soft Actor–Critic (PASAC), which is an unsafe version of PASAC-PIDLag. Both algorithms are employed to train the lane-change strategy to output both discrete lane-change decisions and continuous longitudinal vehicle acceleration. Our simulation results indicate that at a traffic density of 15 vehicles per kilometer (15 veh/km), the PASAC-PIDLag algorithm exhibits superior safety with a collision rate of 0%, outperforming the PASAC algorithm, which has a collision rate of 1%. The generalization assessments reveal that at low traffic density levels, both the PASAC-PIDLag and PASAC algorithms are proficient in attaining zero collision rates. However, at high traffic density levels, although both algorithms result in collisions, PASAC-PIDLag has a much lower collision rate than PASAC.
A Concise Review on Materials for Injection Moulds and Their Conventional and Non-Conventional Machining Processes
Authors: Pedroso André F. V. , Sebbe Naiara P. V. , Silva Francisco J. G. , Campilho Raul D. S. G. , Sales-Contini Rita C. M. , Costa Rúben D. F. S. , Barbosa Marta L. S. , Nogueira Francisca R.
Published: April 2024

Injection moulds are crucial to produce plastic and lightweight metal components. One primary associated challenge is that these may suffer from different types of failures, such as wear and/or cracking, due to the extreme temperatures (T), thermal cycles, and pressures involved in the production process. According to the intended geometry and respective needs, mould manufacturing can be performed with conventional or non-conventional processes. This work focuses on three foremost alloys: AMPCO® (CuBe alloy), INVAR-36® (Fe-Ni alloys, Fe-Ni36), and heat-treated (HT) steels. An insight into the manufacturing processes’ limitations of these kinds of materials will be made, and solutions for more effective machining will be presented by reviewing other published works from the last decade. The main objective is to provide a concise and comprehensive review of the most recent investigations of these alloys’ manufacturing processes and present the machinability challenges from other authors, discovering the prospects for future work and contributing to the endeavours of the injection mould industry. This review highlighted the imperative for more extensive research and development in targeted domains.
Comment on Vishalakshi et al. MHD Hybrid Nanofluid Flow over a Stretching/Shrinking Sheet with Skin Friction: Effects of Radiation and Mass Transpiration. Magnetochemistry 2023, 9, 118
Authors: Pantokratoras Asterios
Published: April 2024

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Fuzzy-Based Road Accident Risk Assessment
Authors: Mogyorósi Péter , Szénási Sándor , Laufer Edit
Published: April 2024

It is necessary to extensively investigate the causes of road accidents with the utmost precision to harness future technological advancements, such as autonomous driving and intelligent accident prevention systems. Nevertheless, since most accidents are attributed to simple human errors, unraveling the complex root-cause factors poses a considerable challenge. This is where fuzzy logic can offer a potential solution: it is essential to understand even seemingly straightforward errors, such as speeding, to identify external factors that could play a pivotal role in future accident prevention. A more in-depth examination and comprehension of elements like road curvature, slope, and their correlation with accidents are necessary. Additionally, it is crucial to explore how the frequency of accidents on specific road segments varies under diverse weather conditions. This article analyzes which curves can be considered more dangerous and the factors that render them risky. The fuzzy model presented in this article is primarily capable of estimating the risk of a given road segment based on its curvature characteristics. The model results presented in the article indicate that sections of the road can become more risky due to multiple curves and curves with a radius of less than 80 m. The model assesses risk based on the physical characteristics of road segments, primarily the curvature radius, while, typically, other road risk assessment models rely on traffic volume and accident counts.
Solid Isotropic Material with Penalization-Based Topology Optimization of Three-Dimensional Magnetic Circuits with Mechanical Constraints
Authors: Houta Zakaria , Huguet Thomas , Lebbe Nicolas , Messine Frédéric
Published: April 2024

Topology optimization is currently enjoying renewed interest thanks to the recent development of 3D printing techniques, which offer the possibility of producing these new complex designs. One of the difficulties encountered in manufacturing topologically optimized magnetostatic structures is that they are not necessarily mechanically stable. In order to take this mechanical constraint into account, we have developed a SIMP-based topology optimization algorithm which relies on numerical simulations of both the mechanical deformation and the magnetostatic behavior of the structure. Two variants are described in this paper, respectively taking into account the compliance or the von Mises constraint. By comparing the designs obtained with those from magnetostatic optimization alone, our approach proves effective in obtaining efficient and robust designs.
Communication-Efficient Zeroth-Order Adaptive Optimization for Federated Learning
Authors: Xie Ping , Gao Xiangrui , Li Fan , Xing Ling , Zhang Yu , Sun Hanxiao
Published: April 2024

Federated learning has become a prevalent distributed training paradigm, in which local devices collaboratively train learning models without exchanging local data. One of the most dominant frameworks of federated learning (FL) is FedAvg, since it is efficient and simple to implement; here, the first-order information is generally utilized to train the parameters of learning models. In practice, however, the gradient information may be unavailable or infeasible in some applications, such as federated black-box optimization problems. To solve the issue, we propose an innovative zeroth-order adaptive federated learning algorithm without using the gradient information, referred to as ZO-AdaFL, which integrates the zeroth-order optimization algorithm into the adaptive gradient method. Moreover, we also rigorously analyze the convergence behavior of ZO-AdaFL in a non-convex setting, i.e., where ZO-AdaFL achieves convergence to a region close to a stationary point at a speed of O(1/T) (T represents the total iteration number). Finally, to verify the performance of ZO-AdaFL, simulation experiments are performed using the MNIST and FMNIST datasets. Our experimental findings demonstrate that ZO-AdaFL outperforms other state-of-the-art zeroth-order FL approaches in terms of both effectiveness and efficiency.
Human Leucocyte Antigen Genetics in Idiosyncratic Drug-Induced Liver Injury with Evidence Based on the Roussel Uclaf Causality Assessment Method
Authors: Teschke Rolf , Danan Gaby
Published: April 2024

The human leucocyte antigen (HLA) allele variability was studied in cohorts of patients with idiosyncratic drug-induced liver injury (iDILI). Some reports showed an association between HLA genetics and iDILI, proposing HLA alleles as a potential risk factor for the liver injury. However, the strength of such assumptions heavily depends on the quality of the iDILI diagnosis, calling for a thorough analysis. Using the PubMed database and Google Science, a total of 25 reports of case series or single cases were retrieved using the terms HLA genes and iDILI. It turned out that in 10/25 reports (40%), HLA genetics were determined in iDILI cases, for which no causality assessment method (CAM) was used or a non-validated tool was applied, meaning the findings were based on subjective opinion, providing disputable results and hence not scoring individual key elements. By contrast, in most iDILI reports (60%), the Roussel Uclaf Causality Assessment Method (RUCAM) was applied, which is the diagnostic algorithm preferred worldwide to assess causality in iDILI cases and represents a quantitative, objective tool that has been well validated by both internal and external DILI experts. The RUCAM provided evidence-based results concerning liver injury by 1 drug class (antituberculotics + antiretrovirals) and 19 different drugs, comprising 900 iDILI cases. Among the top-ranking drugs were amoxicillin–clavulanate (290 cases, HLA A*02:01 or HLA A*30:02), followed by flucloxacillin (255 cases, HLA B*57:01), trimethoprim–sulfamethoxazole (86 cases, HLA B*14:01 or HLA B*14:02), methimazole (40 cases, HLA C*03:02), carbamazepine (29 cases, HLA A*31:01), and nitrofurantoin (26 cases, HLA A*33:01). In conclusion, the HLA genetics in 900 idiosyncratic drug-induced liver injury cases with evidence based on the RUCAM are available for studying the mechanistic steps leading to the injury, including metabolic factors through cytochrome P450 isoforms and processes that activate the innate immune system to the adaptive immune system.
Environmentally Friendly Photothermal Membranes for Halite Recovery from Reverse Osmosis Brine via Solar-Driven Membrane Crystallization
Authors: Aquino Marco , Santoro Sergio , Politano Antonio , D’Andrea Giuseppe , Siciliano Alessio , Straface Salvatore , La Russa Mauro Francesco , Curcio Efrem
Published: April 2024

Modern society and industrial development rely heavily on the availability of freshwater and minerals. Seawater reverse osmosis (SWRO) has been widely adopted for freshwater supply, although many questions have arisen about its environmental sustainability owing to the disposal of hypersaline rejected solutions (brine). This scenario has accelerated significant developments towards the hybridization of SWRO with membrane distillation–crystallization (MD-MCr), which can extract water and minerals from spent brine. Nevertheless, the substantial specific energy consumption associated with MD-MCr remains a significant limitation. In this work, energy harvesting was secured from renewables by hotspots embodied in the membranes, implementing the revolutionary approach of brine mining via photothermal membrane crystallization (PhMCr). This method employs self-heating nanostructured interfaces under solar radiation to enhance water evaporation, creating a carefully controlled supersaturated environment responsible for the extraction of minerals. Photothermal mixed matrix photothermal membranes (MMMs) were developed by incorporating graphene oxide (GO) or carbon black (CB) into polyvinylidene fluoride (PVDF) solubilized in an eco-friendly solvent (i.e., triethyl phosphate (TEP)). MMMs were prepared using non-solvent-induced phase separation (NIPS). The effect of GO or GB on the morphology of MMMs and the photothermal behavior was examined. Light-to-heat conversion was used in PhMCr experiments to facilitate the evaporation of water from the SWRO brine to supersaturation, leading to sodium chloride (NaCl) nucleation and crystallization. Overall, the results indicate exciting perspectives of PhMCr in brine valorization for a sustainable desalination industry.
Modular Finite Element Modeling of Heavy Plate Rolling Processes Using Customized Model Reduction Approaches
Authors: Nemetz Andreas W. , Parteder Erik , Reimer Paula , Kaltenbrunner Thomas , Heise Bodo , Lekue Jagoba , Gross Thomas , Falkner Stefan , Egger Rupert , Zeman Klaus
Published: April 2024

Heavy plates are indispensable semi-finished products. Quality is strongly linked with production, so the rolling process must be performed within well-defined narrow tolerances. To meet this challenge, adequate modeling has become a necessity. In contrast to continuous strip rolling, where the workpiece can be modeled as a semi-infinite strip and 2D modeling can be argued quite well, this strategy is insufficient for the comprehensive modeling of heavy plate rolling. The geometry of the heavy plate favors an inhomogeneous distribution of relevant state variables, such as temperature. In addition, if the process involves longitudinal and spreading passes, the required plate rotation spoils the assumption of a symmetric arrangement that might have been acceptable before rotation. Consequently, the derivation of suitably reduced models is not trivial, and modeling tailored to the specific objective of investigation is of utmost importance. Models intended to resolve the evolution of inhomogeneities in the field variables are demanding and computationally expensive. An effective modular modeling strategy was developed for such models to be used offline. Mutually complementing and interchangeable modules may constitute an efficient modeling strategy valid for the specific subject of interest. The presented approach reduces the enormous cost of complete 3D simulation as much as the model purpose allows for.
Additive Manufacturing of Rare Earth Permanent Magnetic Materials: Research Status and Prospects
Authors: Chen Yun , Xiong Chengyue , Li Yongbing
Published: April 2024

With the rapid development of intelligent manufacturing, modern components are accelerating toward being light weight, miniaturized, and complex, which provides a broad space for the application of rare earth permanent magnet materials. As an emerging near-net-shape manufacturing process, additive manufacturing (AM) has a short process flow and significantly reduces material loss and energy consumption, which brings new possibilities and impetus to the development of rare earth permanent magnetic materials. Here, the applications of AM technology in the field of rare earth permanent magnets in recent years are reviewed and prospected, including laser powder bed fusion (LPBF), fused deposition modeling (FDM), and binder jetting (BJ) techniques. Research has found that the magnetic properties of AM Nd-Fe-B magnets can reach or even exceed the traditional bonded magnets. In addition, in situ magnetic field alignment, in situ grain boundary infiltration, and post-processing methods are effective in enhancing the magnetic properties of AM magnets. These results have laid a good foundation for the development of AM rare earth permanent magnets.
Evaluation of 3D Printing Orientation on Volume Parameters and Mechanical Properties of As-Build TI64ELI
Authors: Lebea Lebogang , Desai Dawood , Ngwangwa Harry , Nemavhola Fulufhelo
Published: April 2024

The discovery of the utility of various titanium alloys as implant biomaterials has resulted in these materials becoming far more popular than other metals in the medical world. However, the production of these materials using additive manufacturing has its own challenges some of those being the surface finish that can be used as an implantology material. As such, the purpose of this study is to evaluate the influence of 3D-printed Ti64ELI on the as-built samples printed at 60°, 90°, and 180° orientations. Such studies are very limited, specifically in the development of the laser shock peening surface modification of dental implants. The study showed that each mechanical test that was performed contributes differently to the printing orientation, e.g., some tests yielded better properties when 180° printing orientation was used, and others had poorer properties when a 180° printing orientation was used. It was observed that 60° testing yielded a micro-hardness value of 349.6, and this value was increased by 0.37% when 90° orientation was measured. The lowest HV value was observed under a 180° orientation with 342.2 HV. The core material volume (Vmc) was 0.05266 mm3/mm2 at a 60° orientation, which increased by 11.48% for the 90° orientation. Furthermore, it was observed that the surface roughness (Sa) at 60° orientation was 43.68 μm. This was further increased by 6% when using the 90° orientation.
Impact of MSMEG5257 Deletion on Mycolicibacterium smegmatis Growth
Authors: He Ping , Zhao Bing , He Wencong , Song Zexuan , Pei Shaojun , Liu Dongxin , Xia Hui , Wang Shengfen , Ou Xichao , Zheng Yang , Zhou Yang , Song Yuanyuan , Wang Yiting , Cao Xiaolong , Xing Ruida , Zhao Yanlin
Published: April 2024

Mycobacterial membrane proteins play a pivotal role in the bacterial invasion of host cells; however, the precise mechanisms underlying certain membrane proteins remain elusive. Mycolicibacterium smegmatis (Ms) msmeg5257 is a hemolysin III family protein that is homologous to Mycobacterium tuberculosis (Mtb) Rv1085c, but it has an unclear function in growth. To address this issue, we utilized the CRISPR/Cas9 gene editor to construct Δmsmeg5257 strains and combined RNA transcription and LC-MS/MS protein profiling to determine the functional role of msmeg5257 in Ms growth. The correlative analysis showed that the deletion of msmeg5257 inhibits ABC transporters in the cytomembrane and inhibits the biosynthesis of amino acids in the cell wall. Corresponding to these results, we confirmed that MSMEG5257 localizes in the cytomembrane via subcellular fractionation and also plays a role in facilitating the transport of iron ions in environments with low iron levels. Our data provide insights that msmeg5257 plays a role in maintaining Ms metabolic homeostasis, and the deletion of msmeg5257 significantly impacts the growth rate of Ms. Furthermore, msmeg5257, a promising drug target, offers a direction for the development of novel therapeutic strategies against mycobacterial diseases.
A Point Mutation in Cassette Relieves the Repression Regulation of CcpA Resulting in an Increase in the Degradation of 2,3-Butanediol in Lactococcus lactis
Authors: Xu Xian , Liu Fulu , Qiao Wanjin , Dong Yujie , Yang Huan , Liu Fengming , Xu Haijin , Qiao Mingqiang
Published: April 2024

In lactic acid bacteria, the global transcriptional regulator CcpA regulates carbon metabolism by repressing and activating the central carbon metabolism pathway, thus decreasing or increasing the yield of certain metabolites to maximize carbon flow. However, there are no reports on the deregulation of the inhibitory effects of CcpA on the metabolism of secondary metabolites. In this study, we identified a single-base mutant strain of Lactococcus lactis N8-2 that is capable of metabolizing 2,3-butanediol. It has been established that CcpA dissociates from the catabolite responsive element (cre) site due to a mutation, leading to the activation of derepression and expression of the 2,3-butanediol dehydrogenase gene cluster (butB and butA). Transcriptome analysis and quantitative polymerase chain reaction (Q-PCR) results showed significant upregulation of transcription of butB and butA compared to the unmutated strain. Furthermore, micro-scale thermophoresis experiments confirmed that CcpA did not bind to the mutated cre. Furthermore, in a bacterial two-plasmid fluorescent hybridization system, it was similarly confirmed that the dissociation of CcpA from cre eliminated the repressive effect of CcpA on downstream genes. Finally, we investigated the differing catalytic capacities of the 2,3-butanediol dehydrogenase gene cluster in L. lactis N8-1 and L. lactis N8-2 for 2,3-butanediol. This led to increased expression of butB and butA, which were deregulated by CcpA repression. This is the first report on the elimination of the deterrent effect of CcpA in lactic acid bacteria, which changes the direction of enzymatic catalysis and alters the direction of carbon metabolism. This provides new perspectives and strategies for metabolizing 2,3-butanediol using bacteria in synthetic biology.
Insights into Chitin-Degradation Potential of Shewanella khirikhana JW44 with Emphasis on Characterization and Function of a Chitinase Gene SkChi65
Authors: Wang Ling , Xue Ming , Yan Rui , Xue Jiawei , Lu Zhipeng , Wen Chongqing
Published: April 2024

Chitin, a polymer of β-1,4-linked N-acetylglucosamine (GlcNAc), can be degraded into valuable oligosaccharides by various chitinases. In this study, the genome of Shewanella khirikhana JW44, displaying remarkable chitinolytic activity, was investigated to understand its chitin-degradation potential. A chitinase gene SkChi65 from this strain was then cloned, expressed, and purified to characterize its enzymatic properties and substrate hydrolysis. Genome analysis showed that, of the 14 genes related to chitin utilization in JW44, six belonged to glycoside hydrolase (GH) families because of their functional domains for chitin binding and catalysis. The recombinant chitinase SkChi65, consisting of 1129 amino acids, was identified as a member of the GH18 family and possessed two chitin-binding domains with a typical motif of [A/N]KWWT[N/S/Q] and one catalytic domain with motifs of DxxDxDxE, SxGG, YxR, and [E/D]xx[V/I]. SkChi65 was heterologously expressed as an active protein of 139.95 kDa best at 37 °C with 1.0 mM isopropyl-β-d-thiogalactopyranoside induction for 6 h. Purified SkChi65 displayed high stability over the ranges of 30–50 °C and pH 5.5–8.0 with optima at 40 °C and pH 7.0. The kinetic parameters Km, Vmax, and kcat of SkChi65 towards colloidal chitin were 27.2 μM, 299.2 μMs−1, and 10,203 s−1, respectively. In addition to colloidal chitin, SkChi65 showed high activity towards glycol chitosan and crystalline chitin. After analysis by thin-layer chromatography, the main products were N,N’-diacetylchitobiose, and GlcNAc with (GlcNAc)2–6 used as substrates. Collectively, SkChi65 could exhibit both exo- and endochitinase activities towards diverse substrates, and strain JW44 has a high potential for industrial application with an excellent capacity for chitin bioconversion.
Development of a Real-Time Recombinase-Aided Amplification Method for the Rapid Detection of Streptococcus equi subsp. equi
Authors: Zu Haoyu , Sun Rongkuan , Li Jiaxin , Guo Xing , Wang Min , Guo Wei , Wang Xiaojun
Published: April 2024

Streptococcus equi subspecies equi (S. equi) is the causative pathogen of strangles in horses, donkeys, and other equine animals. Strangles has spread globally and causes significant losses to the horse industry. In response to the urgent need for effective disease control, this study introduces a novel nucleic acid diagnostic method known as a real-time recombinase-assisted amplification (RAA) assay, developed based on the eqbE gene, for the rapid detection of S. equi nucleic acid. The real-time RAA method employs specifically designed probes and primers targeting the eqbE gene, enhancing the overall specificity and sensitivity of the detection. After efficiency optimization, this real-time RAA method can detect 10 or more copies of nucleic acid within 20 min. The method demonstrates high specificity for S. equi and does not cross-react with other clinically relevant pathogens. Real-time RAA diagnostic performance was evaluated using 98 nasal swab samples collected from horses and compared with the real-time PCR detection method. Results revealed that 64 and 65 samples tested positive for S. equi using real-time RAA and real-time PCR, respectively. The overall agreement between the two assays was 96.94% (95/98), with a kappa value of 0.931 (p < 0.001). Further linear regression analysis indicated a significant correlation in the detection results between the two methods (R2 = 0.9012, p < 0.0001), suggesting that the real-time RAA assay exhibits a detection performance comparable to that of real-time PCR. In conclusion, the real-time RAA assay developed here serves as a highly specific and reliable diagnostic tool for the detection of S. equi in equine samples, offering a potential alternative to real-time PCR methods. In conclusion, the real-time RAA nucleic acid diagnostic method, based on the eqbE gene, offers rapid and accurate diagnosis of S. equi, with the added advantage of minimal equipment requirements, thus contributing to the efficient detection of strangles in horses.
Mineral Acid Co-Extraction in Reactive Extraction of Lactic Acid Using a Thymol-Menthol Deep Eutectic Solvent as a Green Modifier
Authors: Demmelmayer Paul , Ćosić Marija , Kienberger Marlene
Published: April 2024

Carboxylic acids can be isolated from fermentation broths using reactive liquid-liquid extraction, offering an alternative to the environmentally harmful state-of-the-art process of precipitating calcium lactate. To enhance the sustainability of liquid-liquid extraction processes, greener solvents, such as natural deep eutectic solvents, are investigated. However, fermentation broths often exhibit pH values unsuitable for carboxylic acid extraction, which can be adjusted using mineral acids, though mineral acids may be co-extracted. In this study, we systematically examine the co-extraction of hydrochloric, nitric, sulfuric, and phosphoric acid during extraction and back-extraction of lactic acid. The solvent phase consisted of tri-n-octylamine, trioctylphosphine oxide, or tributyl phosphate diluted in a thymol-menthol deep eutectic solvent. The back-extraction was conducted using a diluent swing with p-cymene as the antisolvent and water as the receiving phase. Tri-n-octylamine showed the highest efficiency for lactic acid (up to 29.8%) but also the highest co-extraction of mineral acids (up to 50.9%). In contrast, trioctylphosphine oxide exhibited a lower but more selective lactic acid extraction (5.94%) with low mineral acids co-extraction (0.135%). Overall, the highest co-extraction was observed for phosphoric acid and the lowest for nitric acid. In conclusion, the selected solvent phase composition and mineral acid influence the co-extraction and, thus, final product purity. The successful application of the natural deep eutectic solvent as the modifier enhances the sustainability of liquid–liquid extraction processes.
Hazel Leaf Polyphenol Extract Alleviated Cisplatin-Induced Acute Kidney Injury by Reducing Ferroptosis through Inhibiting Hippo Signaling
Authors: Sun Mingyang , Chang He , Jiang Fangyang , Zhang Wenjing , Yang Qingxuan , Wang Xinhe , Lv Guangfu , Lin He , Luo Haoming , Lin Zhe , Wang Yuchen
Published: April 2024

Derived from hazelnuts, hazel leaf has been utilized in traditional folk medicine for centuries in countries such as Portugal, Sweden, and Iran. In our previous investigations, we conducted a preliminary assessment of the hazel leaf polyphenol extract (referred to as ZP) and identified nine compounds, such as kaempferol and chlorogenic acid, in its composition. ZP has shown promising properties as an antioxidant and anti-inflammatory agent. Our research has revealed that ZP has protective effects against cisplatin-induced acute kidney injury (AKI). We conducted a comprehensive examination of both the pathological and ultrastructural aspects and found that ZP effectively ameliorated renal tissue lesions and mitigated mitochondrial damage. Moreover, ZP significantly suppressed malondialdehyde levels while increasing glutathione and catalase concentrations in the kidneys of AKI-induced mice. ZP decreased the number of apoptotic cells and decreased pro-apoptotic protein expression in the kidneys of mice and human renal tubular epithelial cells (HK-2). Furthermore, treatment with ZP increased the levels of proteins marking anti-ferroptosis, such as GPX4, FTH1, and FSP1, in experiments both in vivo and in vitro. We elucidated the underlying mechanisms of ZP’s actions, revealing its inhibitory effect on Yap phosphorylation and its regulation of Lats expression, which exert a protective influence on the kidneys. Furthermore, we found that inhibiting the Hippo pathway compromised ZP’s nephroprotective effects in both in vitro and in vivo studies. In summary, this research shows that ZP exhibits renoprotective properties, effectively reducing oxidative damage, apoptosis, and ferroptosis in the kidneys by targeting the Hippo pathway.
Investigating the Catalytic Influence of Boron on Ni-Co/Ca Catalysts for Improved Syngas Generation from Rice Straw Pyrolysis
Authors: Wang Jiaxiang , Wang Luqi , Li Yueyao
Published: April 2024

A series of boron-promoted Ni-Co/Ca catalysts were synthesized by the sol–gel method to enhance syngas generation from biomass pyrolysis. The efficiency of these catalysts was evaluated during the pyrolysis of rice straw in a fixed-bed reactor, varying the Ni/Co ratio, boron addition, calcination temperature, and residence time. The catalysts underwent comprehensive characterization using X-ray diffraction (XRD), Brunauer–Emmett–Teller (BET) analysis, scanning electron microscopy (SEM), and hydrogen temperature-programmed reduction (H2-TPR). The results indicated that the Ni-Co/Ca catalysts yielded superior syngas compared to singular Ni or Co catalysts, suggesting a synergistic interplay between nickel and cobalt. The incorporation of 4% boron significantly decreased the particle size of the active metals, enhancing both the catalytic activity and stability. Optimal syngas production was achieved under the following conditions: a biomass-to-catalyst mass ratio of 2:1, a Ni-Co ratio of 1:1, a calcination temperature of 400 °C, a pyrolysis temperature of 800 °C, and a 20 min residence time. These conditions led to a syngas yield of 431.8 mL/g, a 131.28% increase over the non-catalytic pyrolysis yield of 188.6 mL/g. This study not only demonstrates the potential of Ni-Co/Ca catalysts in biomass pyrolysis for syngas production but also provides a foundation for future catalyst performance optimization.
Schiff Base Functionalized Cellulose: Towards Strong Support-Cobalt Nanoparticles Interactions for High Catalytic Performances
Authors: Aitbella Hicham , Belachemi Larbi , Merle Nicolas , Zinck Philippe , Kaddami Hamid
Published: April 2024

A new hybrid catalyst consisting of cobalt nanoparticles immobilized onto cellulose was developed. The cellulosic matrix is derived from date palm biomass waste, which was oxidized by sodium periodate to yield dialdehyde and was further derivatized by grafting orthoaminophenol as a metal ion complexing agent. The new hybrid catalyst was characterized by FT-IR, solid-state NMR, XRD, SEM, TEM, ICP, and XPS. The catalytic potential of the nanocatalyst was then evaluated in the catalytic hydrogenation of 4-nitrophenol to 4-aminophenol under mild experimental conditions in aqueous medium in the presence of NaBH4 at room temperature. The reaction achieved complete conversion within a short period of 7 min. The rate constant was calculated to be K = 8.7 × 10−3 s−1. The catalyst was recycled for eight cycles. Furthermore, we explored the application of the same catalyst for the hydrogenation of cinnamaldehyde using dihydrogen under different reaction conditions. The results obtained were highly promising, exhibiting both high conversion and excellent selectivity in cinnamyl alcohol.
Optical Second Harmonic Generation of Low-Dimensional Semiconductor Materials
Authors: Fu Yue , Liu Zhengyan , Yue Song , Zhang Kunpeng , Wang Ran , Zhang Zichen
Published: April 2024

In recent years, the phenomenon of optical second harmonic generation (SHG) has attracted significant attention as a pivotal nonlinear optical effect in research. Notably, in low-dimensional materials (LDMs), SHG detection has become an instrumental tool for elucidating nonlinear optical properties due to their pronounced second-order susceptibility and distinct electronic structure. This review offers an exhaustive overview of the generation process and experimental configurations for SHG in such materials. It underscores the latest advancements in harnessing SHG as a sensitive probe for investigating the nonlinear optical attributes of these materials, with a particular focus on its pivotal role in unveiling electronic structures, bandgap characteristics, and crystal symmetry. By analyzing SHG signals, researchers can glean invaluable insights into the microscopic properties of these materials. Furthermore, this paper delves into the applications of optical SHG in imaging and time-resolved experiments. Finally, future directions and challenges toward the improvement in the NLO in LDMs are discussed to provide an outlook in this rapidly developing field, offering crucial perspectives for the design and optimization of pertinent devices.
Impact of Nanoparticle Addition on the Surface and Color Properties of Three-Dimensional (3D) Printed Polymer-Based Provisional Restorations
Authors: AlGhamdi Maram A. , Alatiyyah Fatimah M. , Almedarham Rawan F. , Al Dawood Zainab H. , Alshaikhnasser Farah Y. , Alboryh Shaymaa Y. , Khan Soban Q. , Abualsaud Reem , Gad Mohammed M.
Published: April 2024

This study aimed to evaluate and compare the impact of additives such as ZrO2 and SiO2 nanoparticles (ZrO2NP or SiO2NP) on the hardness, surface roughness, and color stability of 3D printed provisional restorations. Two hundred samples in total were printed using 3D printed resins (ASIGA, and NextDent). Each resin was modified with ZrO2NPs or SiO2NPs in two different concentrations (0.5 wt% and 1 wt%), while one group was kept unmodified (n = 10). Disc-shaped (15 × 2.5 mm) samples were designed and printed in accordance with the manufacturer’s recommendation. Printed discs were evaluated for color changes through parameters CIELAB 2000 system (ΔE00), hardness using Vickers hardness test, and surface roughness (Ra) using a noncontact profilometer. After calculating the means and standard deviations, a three-way ANOVA and Tukey post hoc test were performed at α = 0.05. The addition of ZrO2NPs or SiO2NPs to ASIGA and NextDent resins significantly increased the hardness at a given level of concentration (0.5% or 1%) in comparison with pure (p < 0.001), with no significant difference between the two modified groups per resin type (p > 0.05). The highest hardness value was detected in 1% ZrO2NPs with 29.67 ± 2.3. The addition of ZrO2NPs or SiO2NPs had no effect on the Ra (p > 0.05), with 1% ZrO2NPs showing the highest value 0.36 ± 0.04 µm with NextDent resin. ZrO2NPs induced higher color changes (∆E00), ranging from 4.1 to 5.8, while SiO2NPs showed lower values, ranging from 1.01 to 1.85, and the highest mean ∆E00 was observed in the 1% ZrO2NPs group and NextDent resin. The incorporation of ZrO2NPs and SiO2NPs in 3D printed provisional resins increased the hardness without affecting the surface roughness. The optical parameters were significantly affected by ZrO2NPs and less adversely affected by SiO2NPs. Consequently, care must be taken to choose a concentration that will improve the materials’ mechanical performance without detracting from their esthetic value.
Functional Significance of miR-4693-5p in Targeting HIF1α and Its Link to Rheumatoid Arthritis Pathogenesis
Authors: Saquib Mohd , Agnihotri Prachi , Sarkar Ashish , Malik Swati , Mann Sonia , Chakraborty Debolina , Joshi Lovely , Malhotra Rajesh , Biswas Sagarika
Published: April 2024

Rheumatoid arthritis (RA) is a chronic inflammatory autoimmune disease that causes joint inflammation and destruction with an unknown origin. Our study aims to elucidate the molecular mechanism behind HIF1α overexpression in RA. Dysregulated miRNA expressions are known to influence gene behavior, thereby enhancing cell proliferation, inflammation, and resistance to apoptosis, contributing to RA development. Our earlier finding indicated that exogenous miRNA similar to miR-4693-5p may modulate RA-related targets. However, the specific role of miR-4693-5p and its targets in RA remain unexplored. In this study, we found that miR-4693-5p was significantly reduced in PBMCs of RA patients, with evidence suggesting it targets the 3′ UTR of HIF1α, thereby potentially contributing to its overexpression in RA. In vitro overexpression of miR-4693-5p leads to the knockdown of HIF1α, resulting in inhibited expression of Survivin to disrupt apoptosis resistance, inflammation suppression, and a reduction in the total cellular ROS response in SW982 and RAFLS cells. The results were validated using the CIA Rat model. In conclusion, this study provides a crucial foundation for understanding the functional role of miR-4693-5p. These findings improve our understanding and provide novel insights into the molecular mechanisms underlying RA pathogenesis.
Consumption of Feed Supplemented with Oat Beta-Glucan as a Chemopreventive Agent against Colon Cancerogenesis in Rats
Authors: Harasym Joanna , Dziendzikowska Katarzyna , Kopiasz Łukasz , Wilczak Jacek , Sapierzyński Rafał , Gromadzka-Ostrowska Joanna
Published: April 2024

Colorectal cancer (CRC) accounts for 30% of all cancer cases worldwide and is the second leading cause of cancer-related deaths. CRC develops over a long period of time, and in the early stages, pathological changes can be mitigated through nutritional interventions using bioactive plant compounds. Our study aims to determine the effect of highly purified oat beta-glucan on an animal CRC model. The study was performed on forty-five male Sprague–Dawley rats with azoxymethane-induced early-stage CRC, which consumed feed containing 1% or 3% low molar mass oat beta-glucan (OBG) for 8 weeks. In the large intestine, morphological changes, CRC signaling pathway genes (RT-PCR), and proteins (Western blot, immunohistochemistry) expression were analyzed. Whole blood hematology and blood redox status were also performed. Results indicated that the histologically confirmed CRC condition led to a downregulation of the WNT/β-catenin pathway, along with alterations in oncogenic and tumor suppressor gene expression. However, OBG significantly modulated these effects, with the 3% OBG showing a more pronounced impact. Furthermore, CRC rats exhibited elevated levels of oxidative stress and antioxidant enzyme activity in the blood, along with decreased white blood cell and lymphocyte counts. Consumption of OBG at any dose normalized these parameters. The minimal effect of OBG in the physiological intestine and the high activity in the pathological condition suggest that OBG is both safe and effective in early-stage CRC.
Lactobacillus helveticus-Derived Whey-Calcium Chelate Promotes Calcium Absorption and Bone Health of Rats Fed a Low-Calcium Diet
Authors: Hu Wei , Pei Zhiwen , Xia Aonan , Jiang Yang , Yang Bo , Liu Xiaoming , Zhao Jianxin , Zhang Hao , Chen Wei
Published: April 2024

This study investigated the characteristics of Lactobacillus helveticus-derived whey-calcium chelate (LHWCC) and its effect on the calcium absorption and bone health of rats. Fourier-transform infrared spectroscopy showed that carboxyl oxygen atoms, amino nitrogen atoms, and phosphate ions were the major binding sites with calcium in LHWCC, which has a sustained release effect in simulated in vitro digestion. LHWCC had beneficial effects on serum biochemical parameters, bone biomechanics, and the morphological indexes of the bones of calcium-deficient rats when fed at a dose of 40 mg Ca/kg BW for 7 weeks. In contrast to the inorganic calcium supplement, LHWCC significantly upregulated the gene expression of transient receptor potential cation V5 (TRPV5), TRPV6, PepT1, calcium-binding protein-D9k (Calbindin-D9k), and a calcium pump (plasma membrane Ca-ATPase, PMCA1b), leading to promotion of the calcium absorption rate, whereas Ca3(PO4)2 only upregulated the TRPV6 channel in vivo. These findings illustrate the potential of LHWCC as an organic calcium supplement.
Hadronic Light-by-Light Corrections to the Muon Anomalous Magnetic Moment
Authors: Melo Daniel , Reyes Edilson , Fazio Raffaele
Published: April 2024

We review the hadronic light-by-light (HLbL) contribution to the muon anomalous magnetic moment. Upcoming measurements will reduce the experimental uncertainty of this observable by a factor of four; therefore, the theoretical precision must improve accordingly to fully harness such an experimental breakthrough. With regards to the HLbL contribution, this implies a study of the high-energy intermediate states that are neglected in dispersive estimates. We focus on the maximally symmetric high-energy regime and in-quark loop approximation of perturbation theory, following the method of the OPE with background fields proposed by Bijnens et al. in 2019 and 2020. We confirm their results regarding the contributions to the muon g−2. For this, we use an alternative computational method based on a reduction in the full quark loop amplitude, instead of projecting on a supposedly complete system of tensor structures motivated by first principles. Concerning scalar coefficients, mass corrections have been obtained by hypergeometric representations of Mellin–Barnes integrals. By our technique, the completeness of such kinematic singularity/zero-free tensor decomposition of the HLbL amplitude is explicitly checked.
Polymeric Nanocapsules Loaded with Lidocaine: A Promising Formulation for Topical Dental Anesthesia
Authors: da Silva Camila Batista , dos Santos Cleiton Pita , Serpe Luciano , Sanchez Jonny Burga , Ferreira Luiz Eduardo Nunes , de Melo Nathalie Ferreira Silva , Groppo Francisco Carlos , Fraceto Leonardo Fernandes , Volpato Maria Cristina , Franz-Montan Michelle
Published: April 2024

Lidocaine is the most commonly used local anesthetic worldwide, known for its rapid onset and moderate duration of anesthesia. However, it is short-lived and does not effectively promote effective topical anesthesia in the oral cavity when used alone. Our aim was to investigate whether an approximate 50% encapsulation of lidocaine in poly(ε-caprolactone) nanocapsules (LDC-Nano) would be able to increase its permeation and analgesic efficacy and reduce cytotoxicity. In this study, we characterized LDC-Nano and conducted MTT tests with HaCaT cells to assess their in vitro cytotoxicity. Additionally, in vitro permeation assays across the pig esophageal epithelium and the anesthetic efficacy of the hind paw incision model in rats were performed. Plain lidocaine (LDC) was compared with LDC-Nano and lidocaine hydrochloride plus epinephrine (LDC-Epi). The physicochemical characteristics of LDC-Nano were satisfactory (pH: 8.1 ± 0.21; polydispersity index: 0.08 ± 0.01; mean diameter (nm): 557.8 ± 22.7; and encapsulation efficiency (%): 51.8 ± 1.87) and remained stable for up to 4 months. LDC-Nano presented similar in vitro cytotoxicity to LDC but was higher than LDC-Epi (LD50: LDC = 0.48%; LDC-Nano = 0.47%; and LDC-Epi = 0.58%; p < 0.0001). Encapsulation increased the permeability coefficient about 6.6 times and about 7.5 the steady-state flux of lidocaine across the mucosal epithelium. Both encapsulation and epinephrine improved anesthesia duration, with epinephrine demonstrating superior efficacy (100% of animals were anesthetized up to 100, 30, and 20 min when LDC-Epi, LDC-nano, and LDC were used, respectively). Although LDC-Epi demonstrated superior in vivo anesthetic efficacy, the in vitro permeation and cytotoxicity of LDC-Nano indicate promising avenues for future research, particularly in exploring its potential application as a topical anesthetic in the oral cavity.