bluuebunny/crossref_metadata_2025 huggingface dataset:
Study of Heathland Succession, Prescribed Burning, and Future Perspectives at Kringsjå, Norway
Authors: Gjedrem Anna Marie , Log Torgrim
Published: December 2020

The coastal heathland of Western Europe, dominated by Calluna vulgaris L., was previously maintained by prescribed-burning and grazing to the extent that the Calluna became anthropogenically adapted to regular burning cycles. This 5000–6000-year-old land management practice was essential for local biodiversity and created a vegetation free from major wildland fires. In Norway, recent neglect has, however, caused accumulation of live and dead biomass. Invasion of juniper and Sitka spruce has resulted in limited biodiversity and increasing wildland fire fuels. At the Kringsjå cabin and sheep farm, Haugesund, an area of previous fire safe heathland has been restored through fire-agriculture. Kringsjå is located close to several important Viking Age sites and the Steinsfjellet viewpoint, a popular local tourist destination. The motivation for the present study is to analyse this facility and investigate possibilities for synergies between landscape management and tourism as a route to sustainable transitions. The present study compares restored heathland vegetation with unmanaged heathland at Kringsjå. The potential for activities is also analysed based on the proximity to the tourist attractions in the region. The Kringsjå area demonstrates different vegetation conditions depending on level of afforestation, Calluna heath maintenance, and gracing. Within a few minutes’ walk, dense Sitka spruce communities with desert-like forest floor may be compared to native forest floors, Calluna dominated heathland, and grazing fields. It turns out that Kringsjå may become a showcase for resuming prescribed burning and grazing for fire-safe rich landscapes, while offering cultural and historical experiences for all age groups. Moreover, tourism may become a source of income required for supporting ongoing restoration initiatives. To start working on a common vision, preferably aligned with existing "Homeland of the Viking Kings" tourism approach, should be one of the first steps along this path.
Innovative Materials for Construction
Authors: Frigione Mariaenrica , Aguiar José Luís Barroso de
Published: December 2020

Academic and industrial efforts around the world are continuously engaged to develop new smart materials that can provide efficient alternatives to conventional construction materials and improve the energy-efficiency in buildings or are able to upgrade, repair, and protect existing infrastructures [...]
Effect of Nitrided and Nitrocarburised Austenite on Pitting and Crevice Corrosion Resistance of 316 LVM Steel Implants
Authors: Kajzer Anita , Ceglarska Magdalena , Sura Nika , Kajzer Wojciech , Borowski Tomasz , Tarnowski Michał , Pilecki Zbigniew
Published: December 2020

Harmful lesions occur in the body around multielement stabilisers made of AISI 316 LVM (Low Vacuum Melted) steel, caused by products of pitting, fretting or crevice corrosion. Preventing the effect is possible by modifying the surface of the steel implants. Therefore, the goal of the paper is the comparison of the mechanical and physiochemical properties of plates for treating deformations of the anterior chest wall made of AISI 316 LVM steel, subjected to diffusion and sterilisation processes and exposed to Ringer’s solution. The surface of the implants was subjected to electrochemical polishing, chemical passivation and, in order to modify their properties, nitrocarburised and nitrided diffusion layers were created on selected stabilisers under glow discharge conditions with the use of an active screen at a temperature of 420 °C, over 60 min. The conducted studies involved the examination of the microstructure of the formed layers, surface roughness testing, analysis of contact angles and surface free energy, examination of resistance to pitting and crevice corrosion and examination of nanohardness. On the basis of the results of the conducted studies, it was established that the most advantageous set of properties after sterilisation and exposure to Ringer’s solution was displayed by implants with a formed diffusion nitrocarburised layer.
Engineering Properties of Waste Sawdust-Based Lightweight Alkali-Activated Concrete: Experimental Assessment and Numerical Prediction
Authors: Alabduljabbar Hisham , Huseien Ghasan Fahim , Sam Abdul Rahman Mohd , Alyouef Rayed , Algaifi Hassan Amer , Alaskar Abdulaziz
Published: December 2020

Alkali activated concretes have emerged as a prospective alternative to conventional concrete wherein diverse waste materials have been converted as valuable spin-offs. This paper presents a wide experimental study on the sustainability of employing waste sawdust as a fine/coarse aggregate replacement incorporating fly ash (FA) and granulated blast furnace slag (GBFS) to make high-performance cement-free lightweight concretes. Waste sawdust was replaced with aggregate at 0, 25, 50, 75, and 100 vol% incorporating alkali binder, including 70% FA and 30% GBFS. The blend was activated using a low sodium hydroxide concentration (2 M). The acoustic, thermal, and predicted engineering properties of concretes were evaluated, and the life cycle of various mixtures were calculated to investigate the sustainability of concrete. Besides this, by using the available experimental test database, an optimized Artificial Neural Network (ANN) was developed to estimate the mechanical properties of the designed alkali-activated mortar mixes depending on each sawdust volume percentage. Based on the findings, it was found that the sound absorption and reduction in thermal conductivity were enhanced with increasing sawdust contents. The compressive strengths of the specimens were found to be influenced by the sawdust content and the strength dropped from 65 to 48 MPa with the corresponding increase in the sawdust levels from 0% up to 100%. The results also showed that the emissions of carbon dioxide, energy utilization, and outlay tended to drop with an increase in the amount of sawdust and show more the lightweight concrete to be more sustainable for construction applications.
Environmentally-Benign Phytic Acid-Based Multilayer Coating for Flame Retardant Cotton
Authors: Magovac Eva , Jordanov Igor , Grunlan Jaime C. , Bischof Sandra
Published: December 2020

Chemically bleached cotton fabric was treated with phytic acid (PA), chitosan (CH) and urea by means of layer-by-layer (LbL) deposition to impart flame retardant (FR) behavior using only benign and renewable molecules. Samples were treated with 8, 10, 12 and 15 bilayers (BL) of anionic PA and cationic CH, with urea mixed into the aqueous CH solution. Flammability was evaluated by measuring limiting oxygen index (LOI) and through vertical flame testing. LOI values are comparable to those obtained with commercial flame-retardant finishes, and applying 10 or more bilayers renders cotton self-extinguishing and able to pass the vertical flame test. Microscale combustion calorimeter (MCC) measurements show the average reduction of peak heat release rate (pHRR) of all treated fabrics of ~61% and the reduction of total heat release (THR) of ~74%, in comparison to untreated cotton. Decomposition temperatures peaks (T1max) measured by thermogravimetric analyzer (TG) decreased by approximately 62 °C, while an average residue at 650 °C is ~21% for 10 and more bilayers. Images of post-burn char indicate that PA/CH-urea treatment is intumescent. The ability to deposit such a safe and effective FR treatment, with relatively few layers, makes LbL an alternative to current commercial treatments.
Fracture Models and Effect of Fibers on Fracture Properties of Cementitious Composites—A Review
Authors: Zhang Peng , Yang Yonghui , Wang Juan , Jiao Meiju , Ling Yifeng
Published: December 2020

Cementitious composites have good ductility and pseudo-crack control. However, in practical applications of these composites, the external load and environmental erosion eventually form a large crack in the matrix, resulting in matrix fracture. The fracture of cementitious composite materials causes not only structural insufficiency, but also economic losses associated with the maintenance and reinforcement of cementitious composite components. Therefore, it is necessary to study the fracture properties of cementitious composites for preventing the fracture of the matrix. In this paper, a multi-crack cracking model, fictitious crack model, crack band model, pseudo-strain hardening model, and double-K fracture model for cementitious composites are presented, and their advantages and disadvantages are analyzed. The multi-crack cracking model can determine the optimal mixing amount of fibers in the matrix. The fictitious crack model and crack band model are stress softening models describing the cohesion in the fracture process area. The pseudo-strain hardening model is mainly applied to ductile materials. The double-K fracture model mainly describes the fracture process of concrete. Additionally, the effects of polyvinyl alcohol (PVA) fibers and steel fibers (SFs) on the fracture properties of the matrix are analyzed. The fracture properties of cementitious composite can be greatly improved by adding 1.5–2% PVA fiber or 4% steel fiber (SF). The fracture property of cementitious composite can also be improved by adding 1.5% steel fiber and 1% PVA fiber. However, there are many problems to be solved for the application of cementitious composites in actual engineering. Therefore, further research is needed to solve the fracture problems frequently encountered in engineering.
Correlated Functional Models with Derivative Information for Modeling Microfading Spectrometry Data on Rock Art Paintings
Authors: Riutort-Mayol Gabriel , Gómez-Rubio Virgilio , Lerma José Luis , del Hoyo-Meléndez Julio M.
Published: December 2020

Rock art paintings present high sensitivity to light, and an exhaustive evaluation of the potential color degradation effects is essential for further conservation and preservation actions on these rock art systems. Microfading spectrometry (MFS) is a technique that provides time series of stochastic observations that represent color fading over time at the measured points on the surface under study. In this work, a reliable and robust modeling framework for a short and greatly fluctuating observation dataset collected over the surfaces of rock art paintings located on the walls of Cova Remigia in Ares del Maestrat, Castellón, Spain, is presented. The model is based on a spatially correlated spline-based time series model that takes into account prior information in the form of model derivatives to guarantee monotonicity and long-term saturation for predictions of new color fading estimates at unobserved locations on the surface. The correlation among the (spatially located) time series is modeled by defining Gaussian process (GP) priors over the spline coefficients across time series. The goal is to obtain a complete spatio-temporal mapping of color fading estimates for the study area, which results in very important and useful information that will potentially serve to create better policies and guidelines for heritage preservation and sustainable rock art cultural tourism.
Mathematical Support for Financing Social Innovations
Authors: Popov Evgeny , Veretennikova Anna , Safronova Alisa
Published: December 2020

The use of socially innovative projects for solving social problems by actively involving civil society is a promising and much sought-after area of social development. However, the priority of social goals over economic outcomes in the implementation of such projects significantly limits the speed and effectiveness of their implementation. In this connection, the use of a mathematical tool for the financing and resource provision of social innovations creates new opportunities in terms of the assessment and development of such projects. In order to develop and substantiate tools for the mathematical support of financing social innovations, the role of the collaborative economy in the development of social innovations initiated from below is substantiated. The proposed mathematical toolkit includes a linear algorithm describing the logic of the developed approach, a methodology for assessing socially-innovative projects based on an adapted McKinsey matrix, a methodology for assessing the institutional environment, as well as a mapping of project correspondences in an adapted McKinsey matrix along with collaborative economic tools recommended for resource provision. The described set of collaborative economy tools is recommended for use in the development and implementation of social innovations. The mathematically-described algorithm proposed by the authors is aimed at developing resource provision strategies for social projects by evaluating their competitiveness and attractiveness in terms of the social function they perform while taking the characteristics of the particular institutional environment into consideration. The result of applying this algorithm comprises a set of collaborative economy tools for use in the development and implementation of socially-innovative projects. The application of this algorithm is shown on the example of an evaluation of ten projects implemented in the Ural region and applying for assistance from support funds. The theoretical significance of the proposed results lies in the development of methodological tools for assessing socially-innovative projects. The practical significance lies in the possibility of applying the obtained results in the development of an online calculator used to assist in forming a social project resource provision strategy.
Generalized Market Uncertainty Measurement in European Stock Markets in Real Time
Authors: Uribe Jorge M. , Guillen Montserrat
Published: December 2020

We estimate generalized market uncertainty indicators for the stock markets of eight European countries greatly affected by the recent Covid-19 crisis and the economic measures implemented for its containment and mitigation. Our statistics emphasize the difference between risk and uncertainty, in the aggregate, and provide readily and easily interpretable estimates, in real time, which are relevant for market participants and regulators. We show that generalized uncertainty in Europe was, indeed, at historically high levels in the wake of the recent public health crisis before the large interventions by the European Central Bank, the Fed, and the Bank of England, but also that, for some markets, recently recorded uncertainty levels were still lower than those recorded during the Global Financial Crisis, which puts things into perspective. We also show that uncertainty shocks are extremely persistent, but such persistence varies greatly across countries. The period needed for the markets to absorb half of the shock lies between less than a year and two and a half years.
Chemical Diversity of Soft Coral Steroids and Their Pharmacological Activities
Authors: Ermolenko Ekaterina V. , Imbs Andrey B. , Gloriozova Tatyana A. , Poroikov Vladimir V. , Sikorskaya Tatyana V. , Dembitsky Valery M.
Published: December 2020

The review is devoted to the chemical diversity of steroids produced by soft corals and their determined and potential activities. There are about 200 steroids that belong to different types of steroids such as secosteroids, spirosteroids, epoxy- and peroxy-steroids, steroid glycosides, halogenated steroids, polyoxygenated steroids and steroids containing sulfur or nitrogen heteroatoms. Of greatest interest is the pharmacological activity of these steroids. More than 40 steroids exhibit antitumor and related activity with a confidence level of over 90 percent. A group of 32 steroids shows anti-hypercholesterolemic activity with over 90 percent confidence. Ten steroids exhibit anti-inflammatory activity and 20 steroids can be classified as respiratory analeptic drugs. Several steroids exhibit rather rare and very specific activities. Steroids exhibit anti-osteoporotic properties and can be used to treat osteoporosis, as well as have strong anti-eczemic and anti-psoriatic properties and antispasmodic properties. Thus, this review is probably the first and exclusive to present the known as well as the potential pharmacological activities of 200 marine steroids.
Origin of the Inclusions in Production-Scale Electrodes, ESR Ingots, and PESR Ingots in a Martensitic Stainless Steel
Authors: Sjöqvist Persson Ewa , Karasev Andrey , Mitchell Alec , Jönsson Pär G.
Published: December 2020

The focus of the study was to define the origin of the inclusions in production-scale electro-slag remelting, (ESR) and electro-slag remelting under a protected pressure controlled atmosphere, (PESR), ingots. The inclusion characteristics in production samples were studied using both polished sample surfaces (two-dimensional (2-D) investigations) and inclusions extracted from steel samples by electrolytic extraction (three-dimensional (3-D) investigations) using SEM in combination with EDS. The results were compared to results from previously reported laboratory-, pilot-, and production-scale trials including electrode, remelted, and conventional ingots. The results show that primary, semi-secondary, and secondary inclusions exist in the remelted ingots. The most probable inclusion to survive from the electrode is a MgO-Al2O3 (spinel). It was also found that the ESR/PESR process slag acts in a similar way to a calcium treatment modification of alumina inclusions. On the whole, the most significant finding is that the overall cleanliness of the electrode including the inclusions in the electrode has an influence on the inclusion content of the ESR and PESR ingots.
Diffusion Bonding of High-Alloyed Tool Steels with Maraging and Precipitation Hardening Steels
Authors: Göbl Michael , Eder Harald , Prestl Aude , Siller Ingo , Schnitzer Ronald
Published: December 2020

Steel composites for application as protective plates were produced via diffusion bonding. Cold work tool steels were combined with a precipitation hardening steel or a maraging steel using a deformation dilatometer and a vacuum furnace at 1150 °C for 8 h in total. Subsequently, a heat treatment was applied to achieve the final mechanical properties. The microstructure of the interface was investigated by optical microscopy, scanning electron microscopy, electron backscatter diffraction, energy dispersive X-ray spectroscopy and hardness measurements. The results are compared with a simulation of the diffusion of elements performed by MatCalc. Both composites achieve high hardness near the surface of the cold work tool steels, which can have a positive effect on the destruction of projectiles on impact. The influence of carbon diffusion can be observed up to a depth of 3 mm from the interface. As a result of carbon diffusion, soft zones are formed on both sides of the interface, which can be attributed to decarburization, phase transformation and carbide formation. The tough back layer is designed to absorb the remaining energy of the projectile. The combination of a hard front layer and a tough rear layer provides an optimal combination of materials against ballistic threats.
Development of Provesicular Nanodelivery System of Curcumin as a Safe and Effective Antiviral Agent: Statistical Optimization, In Vitro Characterization, and Antiviral Effectiveness
Authors: Badria Farid A. , Abdelaziz Abdelaziz E. , Hassan Amira H. , Elgazar Abdullah A. , Mazyed Eman A.
Published: December 2020

Curcumin is a natural compound that has many medical applications. However, its low solubility and poor stability could impede its clinical applications. The present study aimed to formulate dry proniosomes to overcome these pitfalls and improve the therapeutic efficacy of Curcumin. Curcumin-loaded proniosomes were fabricated by the slurry method according to 32 factorial design using Design-Expert software to demonstrate the impact of different independent variables on entrapment efficiency (EE%) and % drug released after 12 h (Q12h). The optimized formula (F5) was selected according to the desirability criteria. F5 exhibited good flowability and appeared, after reconstitution, as spherical nanovesicles with EE% of 89.94 ± 2.31% and Q12h of 70.89 ± 1.62%. F5 demonstrated higher stability and a significant enhancement of Q12h than the corresponding niosomes. The docking study investigated the ability of Curcumin to bind effectively with the active site of DNA polymerase of Herpes simplex virus (HSV). The antiviral activity and the safety of F5 were significantly higher than Curcumin. F5 improved the safety of Acyclovir (ACV) and reduced its effective dose that produced a 100% reduction of viral plaques. Proniosomes could be promising stable carriers of Curcumin to be used as a safe and efficient antiviral agent.
Isolation of a Nitromethane Anion in the Calix-Shaped Inorganic Cage
Authors: Kikukawa Yuji , Kitajima Hiromasa , Kuwajima Sho , Hayashi Yoshihito
Published: December 2020

A calix-shaped polyoxometalate, [V12O32]4− (V12), stabilizes an anion moiety in its central cavity. This molecule-sized container has the potential to control the reactivity of an anion. The highly-reactive cyanate is smoothly trapped by V12 to form [V12O32(CN)]5−. In the CH3NO2 solution, cyanate abstracts protons from CH3NO2, and the resultant CH2NO2− is stabilized in V12 to form [V12O32(CH2NO2)]5− (V12(CH2NO2)). A crystallographic analysis revealed the double-bond characteristic short bond distance of 1.248 Å between the carbon and nitrogen atoms in the nitromethane anion in V12. 1H and 13C NMR studies showed that the nitromethane anion in V12 must not be exchanged with the nitromethane solvent. Thus, the V12 container restrains the reactivity of anionic species.
Supramolecular Solvent-Based Microextraction of Selected Anticonvulsant and Nonsteroidal Anti-Inflammatory Drugs from Sediment Samples
Authors: Bajkacz Sylwia , Adamczewska Paulina , Kokoszka Klaudia , Kycia-Słocka Elżbieta , Sochacki Adam , Felis Ewa
Published: December 2020

The increase in the production and consumption of pharmaceuticals increases their presence in the global environment, which may result in direct threats to living organisms. For this reason, there is a need for new methods to analyze drugs in environmental samples. Here, a new procedure for separating and determining selected drugs (diclofenac, ibuprofen, and carbamazepine) from bottom sediment and water samples was developed. Drugs were determined by ultra-high performance liquid chromatography coupled with an ultraviolet detector (UHPLC-UV). In this work, a universal and single-step sample treatment, based on supramolecular solvents (SUPRAS), was proposed to isolate selected anticonvulsants and nonsteroidal anti-inflammatory drugs (NSAIDs) from sediment samples. The following parameters were experimentally selected: composition of the supramolecular solvent (composition THF:H2O (v/v), amount of decanoic acid), volume of extractant, sample mass, extraction time, centrifugation time, and centrifugation speed. Finally, the developed procedure was validated. A Speedisk procedure was also developed to extract selected drugs from water samples. The recovery of analytes using the SUPRAS procedure was in the range of 88.8–115%, while the recoveries of the Speedisk solid-phase extraction procedure ranged from 81.0–106%. The effectiveness of the sorption of the tested drugs by sediment was also examined.
Angular Regioselectivity in the Reactions of 2-Thioxopyrimidin-4-ones and Hydrazonoyl Chlorides: Synthesis of Novel Stereoisomeric Octahydro[1,2,4]triazolo[4,3-a]quinazolin-5-ones
Authors: Said Awad I. , Palkó Márta , Haukka Matti , Fülöp Ferenc
Published: December 2020

The regioselective synthesis of cis and trans stereoisomers of variously functionalized octahydro[1,2,4]triazolo[4,3-a]quinazolin-5-ones was performed. The 2-thioxopyrimidin-4-ones used in the synthesis reacted with hydrazonoyl chlorides in a regioselective manner to produce the angular regioisomers [1,2,4]triazolo[4,3-a]quinazolin-5-ones rather than the linear isomers [1,2,4]triazolo[4,3-a]quinazolin-5-ones. The synthesis process took place with electronic control. The angular regiochemistry of the products was confirmed by X-ray experiments and two-dimensional NMR studies.
The Mediating Effect of Insecure Adult Attachment on the Relationship between Smartphone Addiction and Self-Directed Learning in University Students
Authors: Park Inhee , Kim Sinhyang , Suh Yeonok
Published: December 2020

(1) Purpose: This study aimed to explore the mediating effect of insecure adult attachment on the relationship between smartphone addiction and self-directed learning in university students. (2) Methods: In total, 235 university students participated in this study. Data analysis was carried out through a three-stage verification procedure: Sobel test using technical statistics, Pearson correlation, and multiple regression analysis. (3) Results: Smartphone addiction was positively correlated with insecure adult attachment, attachment anxiety, and self-directed learning, whereas self-directed learning had a significant negative correlation with insecure adult attachment and attachment anxiety. Insecure adult attachment had a mediating effect on the relationship between smartphone addiction and self-directed learning. (4) Conclusion: Higher levels of smartphone addiction indicated higher levels of insecure adult attachment and reduced self-directed learning ability. Therefore, while the prevention of smartphone addiction is critical for improving self-directed learning skills, programs should be developed to foster the formation of secure adult attachment among university students.
Design and Characterization of a Novel Tool for the Antigenic Enrichment of Actinobacillus pleuropneumoniae Outer Membrane
Authors: Antenucci Fabio , Ovsepian Armen , Wrobel Agnieszka , Winther-Larsen Hanne Cecilie , Bojesen Anders Miki
Published: December 2020

Production and isolation of recombinant proteins are costly and work-intensive processes, especially in immunology when tens or hundreds of potential immunogens need to be purified for testing. Here we propose an alternative method for fast screening of immunogen candidates, based on genetic engineering of recombinant bacterial strains able to express and expose selected antigens on their outer membrane. In Actinobacillus pleuropneumoniae, a Gram-negative porcine pathogen responsible for extensive economic losses worldwide, we identified a conserved general secretion pathway (GSP) domain in the N-terminal part of the outer membrane protein ApfA (ApfA stem: ApfAs). ApfAs was used as an outer membrane anchor, to which potential immunogens can be attached. To enable confirmation of correct positioning, ApfAs, was cloned in combination with the modified acyl carrier protein (ACP) fluorescent tag ACP mini (ACPm) and the putative immunogen VacJ. The chimeric construct was inserted in the pMK-express vector, subsequently transformed into A. pleuropneumoniae for expression. Flow cytometry, fluorescence imaging and mass spectrometry analysis were employed to demonstrate that the outer membrane of the transformed strain was enriched with the chimeric ApfAs-ACPm-VacJ antigen. Our results confirmed correct positioning of the chimeric ApfAs-ACPm-VacJ antigen and supported this system’s potential as platform technology enabling antigenic enrichment of the outer membrane of A. pleuropneumoniae.
Application of Gum Arabic and Methyl Cellulose Coatings Enriched with Thyme Oil to Maintain Quality and Extend Shelf Life of “Acco” Pomegranate Arils
Authors: Kawhena Tatenda Gift , Tsige Alemayehu Ambaw , Opara Umezuruike Linus , Fawole Olaniyi Amos
Published: December 2020

The effects of gum arabic (GA; 1.5% w/v) and methyl cellulose (MC; 1% w/v) enriched with thyme oil (TO; 0.25 and 0.5% v/v) on the quality of “Acco” pomegranate arils were studied. Coating treatments, namely, MC, MC + TO0.5%, MC + TO0.25%, GA, GA + TO0.5% and GA + TO0.25% were applied on arils by dipping, and evaluations were made on physicochemical and microbiological quality, phytochemicals and antioxidant capacity of arils stored (5 ± 1 °C, 95 ± 2% RH) for 16 days. Dipping arils in GA or MC, both containing TO (0.5% v/v) significantly (p < 0.0001) reduced weight loss and enhanced antioxidant activity (FRAP) (p = 0.0014). However, dipping arils in GA combined with TO (0.25% v/v) had the highest influence on reducing aril respiration rate compared with other treatments. Overall, results showed that application of coatings (GA + TO0.5% and GA + TO0.25%) reduced total yeast and mould and total plate counts and maintained quality up to 8 days of storage. These findings suggest that either GA + TO0.5% or GA + TO0.25% coatings have the capability to extend storage life of “Acco” pomegranate arils.
A Novel Proximity Biotinylation Assay Based on the Self-Associating Split GFP1–10/11
Authors: Kesari Aditi S. , Aryal Uma K. , LaCount Douglas J.
Published: December 2020

Proximity biotinylation was developed to detect physiologically relevant protein–protein interactions in living cells. In this method, the protein of interest is tagged with a promiscuous biotin ligase, such as BioID or BioID2, which produces activated biotin that reacts with nearby proteins; these proteins can subsequently be purified and identified by mass spectrometry. Here we report a novel modification of this technique by combining it with a self-associating split-GFP system in which we exploit the high-affinity interaction between GFP1–10 and GFP11 to recruit BioID2 to the protein of interest. As a test case, we fused GFP11 to clathrin light chain (CLTB) and BioID2 to GFP1–10. Co-expression of GFP11-CLTB and BioID2-GFP1–10 yielded a green fluorescent complex that co-localized with clathrin heavy chain. To facilitate removal of non-specifically biotinylated proteins, we generated an inducible cell line expressing BioID2-GFP1–10. Proximity biotinylation in this cell line with GFP11-CLTB yielded a higher percentage of biologically relevant interactions than direct fusion of BioID2 to CLTB. Thus, this system can be used to monitor expression and localization of BioID bait proteins and to identify protein–protein interactions.
Image Guided Visual Tracking Control System for Unmanned Multirotor Aerial Vehicle with Uncertainty
Authors: Islam Shafiqul , Mukhtar Husameldin , Dias Jorge
Published: December 2020

This paper presents a wavelet-based image guided tracking control system for unmanned multirotor aerial vehicle system with the presence of uncertainty. The visual signals for the visual tracking process are developed by using wavelet coefficients. The design uses a multiresolution interaction matrix with half and details images to relate the time-variation of wavelet coefficients with the velocity of the aerial vehicle and controller. The proposed design is evaluated on a virtual quadrotor aerial vehicle system to demonstrate the effectiveness of the wavelet-based visual tracking system without using an image processing unit in the presence of uncertainty. In contrast to the classical visual tracking technique, the wavelet-based method does not require an image processing task.
Undoped and Nickel-Doped Zinc Oxide Thin Films Deposited by Dip Coating and Ultrasonic Spray Pyrolysis Methods for Propane and Carbon Monoxide Sensing Applications
Authors: Karthik Tangirala Venkata Krishna , Olvera María de la Luz , Maldonado Arturo , Biswal Rajesh Roshan , Gómez-Pozos Heberto
Published: December 2020

Undoped and nickel-doped zinc oxide thin films were deposited on sodalime glass substrates by utilizing dip coating and ultrasonic spray pyrolysis deposition techniques. In both cases zinc acetate and nickel acetylacetonate were used as zinc precursor and nickel dopant source, respectively. XRD analysis confirms the ZnO wurtzite structure with (002) as the preferential orientation.SEM studies show the formation of two types of morphologies, primarily a porous spherical grains with a grain size distribution from 40 to 150 nm and another, rose-like structures with size distribution from 30 to 200 nm, based on different deposition techniques utilized. The elemental depth profiles across the films were investigated by the secondary-ion mass spectrometry (SIMS). Different gas sensing responses of all ZnO films were obtained for both propane and carbon monoxide gases, at different gas concentrations and operating temperatures. The highest sensing response (~6) for undoped ZnO films was obtained for films deposited by ultrasonic spray pyrolysis (USP). Nevertheless, the highest sensing response (~4 × 104) for doped ZnO films was obtained for films deposited by dip coating method. The behavior of sensing responses is explained in detail based on the morphological properties and the amount of Ni impurities incorporated into the crystal lattice.
Assessing the Trend of the Trophic State of Lake Ladoga Based on Multi-Year (1997–2019) CMEMS GlobColour-Merged CHL-OC5 Satellite Observations
Authors: Gbagir Augustine-Moses Gaavwase , Colpaert Alfred
Published: December 2020

The trophic state of Lake Ladoga was studied during the period 1997–2019, using the Copernicus Marine Environmental Monitoring Service (CMEMS) GlobColour-merged chlorophyll-a OC5 algorithm (GlobColour CHL-OC5) satellite observations. Lake Ladoga, in general, is mesotrophic but certain parts of the lake have been eutrophic since the 1960s due to the discharge of wastewater from industrial, urban, and agricultural sources. Since then, many ecological assessments of the Lake’s state have been made. These studies have indicated that various changes are taking place in the lake and continuous monitoring of the lake is essential to update the current knowledge of its state. The aim of this study was to assess the long-term trend in chl-a in Lake Ladoga. The results showed a gradual reduction in chl-a concentration, indicating a moderate improvement. Chl-a concentrations (minimum-maximum values) varied spatially. The shallow southern shores did not show any improvement while the situation in the north is much better. The shore areas around the functioning paper mill at Pitkäranta and city of Sortavala still show high chl-a values. These findings provide a general reference on the current trophic state of Lake Ladoga that could contribute to improve policy and management strategies. It is assumed that the present warming trend of surface water may result in phytoplankton growth increase, thus partly offsetting a decrease in nutrient load. Precipitation is thought to be increasing, but the influence on water quality is less clear. Future studies could assess the current chemical composition to determine the state of water quality of Lake Ladoga.
Deep Neural Network for Slip Detection on Ice Surface
Authors: Wu Kent , He Suzy , Fernie Geoff , Roshan Fekr Atena
Published: December 2020

Slip-induced falls are among the most common causes of major occupational injuries and economic loss in Canada. Identifying the risk factors associated with slip events is key to developing preventive solutions to reduce falls. One factor is the slip-resistance quality of footwear, which is fundamental to reducing the number of falls. Measuring footwear slip resistance with the recently developed Maximum Achievable Angle (MAA) test requires a trained researcher to identify slip events in a simulated winter environment. The human capacity for information processing is limited and human error is natural, especially in a cold environment. Therefore, to remove conflicts associated with human errors, in this paper a deep three-dimensional convolutional neural network is proposed to detect the slips in real-time. The model has been trained by a new dataset that includes data from 18 different participants with various clothing, footwear, walking directions, inclined angles, and surface types. The model was evaluated on three types of slips: Maxi-slip, midi-slip, and mini-slip. This classification is based on the slip perception and recovery of the participants. The model was evaluated based on both 5-fold and Leave-One-Subject-Out (LOSO) cross validation. The best accuracy of 97% was achieved when identifying the maxi-slips. The minimum accuracy of 77% was achieved when classifying the no-slip and mini-slip trials. The overall slip detection accuracy was 86% with sensitivity and specificity of 81% and 91%, respectively. The overall accuracy dropped by about 2% in LOSO cross validation. The proposed slip detection algorithm is not only beneficial for footwear manufactures to improve their footwear slip resistance quality, but it also has other potential applications, such as improving the slip resistance properties of flooring in healthcare facilities, commercial kitchens, and oil drilling platforms.
Multiplexed Nanobiosensors: Current Trends in Early Diagnostics
Authors: Jarockyte Greta , Karabanovas Vitalijus , Rotomskis Ricardas , Mobasheri Ali
Published: December 2020

The ever-growing demand for fast, cheap, and reliable diagnostic tools for personalised medicine is encouraging scientists to improve existing technology platforms and to create new methods for the detection and quantification of biomarkers of clinical significance. Simultaneous detection of multiple analytes allows more accurate assessment of changes in biomarker expression and offers the possibility of disease diagnosis at the earliest stages. The concept of multiplexing, where multiple analytes can be detected in a single sample, can be tackled using several types of nanomaterial-based biosensors. Quantum dots are widely used photoluminescent nanoparticles and represent one of the most frequent choices for different multiplex systems. However, nanoparticles that incorporate gold, silver, and rare earth metals with their unique optical properties are an emerging perspective in the multiplexing field. In this review, we summarise progress in various nanoparticle applications for multiplexed biomarkers.
Application of Correlation Analysis for Assessment of Infrasound Signals Emission by Wind Turbines
Authors: Boczar Tomasz , Zmarzły Dariusz , Kozioł Michał , Wotzka Daria
Published: December 2020

The study reported in this paper is concerned with areas related to developing methods of measuring, processing and analyzing infrasound noise caused by operation of wind farms. The paper contains the results of the correlation analysis of infrasound signals generated by a wind turbine with a rated capacity of 2 MW recorded by three independent measurement setups comprising identical components and characterized by the same technical parameters. The measurements of infrasound signals utilized a dedicated measurement system called INFRA, which was developed and built by KFB ACOUSTICS Sp. z o.o. In particular, the scope of the paper includes the results of correlation analysis in the time domain, which was carried out using the autocovariance function separately for each of the three measuring setups. Moreover, the courses of the cross-correlation function were calculated separately for each of the potential combinations of infrasound range recorded by the three measuring setups. In the second stage, a correlation analysis of the recorded infrasound signals in the frequency domain was performed, using the coherence function. In the next step, infrasound signals recorded in three setups were subjected to time-frequency transformations. In this part, the waveforms of the scalograms were determined by means of continuous wavelet transform. Wavelet coherence waveforms were calculated in order to determine the level of the correlation of the obtained dependencies in the time-frequency domain. The summary contains the results derived from using correlation analysis methods in the time, frequency and time-frequency domains.
Motherhood in Europe: An Examination of Parental Leave Regulations and Breastfeeding Policy Influences on Breastfeeding Initiation and Duration
Authors: Vanderlinden Karen , Buffel Veerle , Van de Putte Bart , Van de Velde Sarah
Published: December 2020

This study examines how European variation in breastfeeding initiation and duration rates is related to the presence of baby-friendly hospitals, the international code of marketing of breast-milk substitutes, and different constellations of maternal, paternal, and parental leave. We use Eurobarometer data (2005) to compare initiation and duration levels across 21 European countries within a multilevel regression framework. We find that countries play a significant role in determining breastfeeding through their different social policies. Breastfeeding practices across different leave regulation models differ substantially. We conclude that ongoing changes in paid maternity and parental leave length combined with uptake flexibility and paternal involvement help determine breastfeeding rates and should put infant feeding issues on governmental policy agendas across European countries.
Cationic Polymers for the Delivery of the Ebola DNA Vaccine Encoding Artificial T-Cell Immunogen
Authors: Karpenko Larisa I. , Apartsin Evgeny K. , Dudko Sergei G. , Starostina Ekaterina V. , Kaplina Olga N. , Antonets Denis V. , Volosnikova Ekaterina A. , Zaitsev Boris N. , Bakulina Anastasiya Yu. , Venyaminova Aliya G. , Ilyichev Alexander A. , Bazhan Sergei I.
Published: December 2020

Background: According to current data, an effective Ebola virus vaccine should induce both humoral and T-cell immunity. In this work, we focused our efforts on methods for delivering artificial T-cell immunogen in the form of a DNA vaccine, using generation 4 polyamidoamine dendrimers (PAMAM G4) and a polyglucin:spermidine conjugate (PG). Methods: Optimal conditions were selected for obtaining complexes of previously developed DNA vaccines with cationic polymers. The sizes, mobility and surface charge of the complexes with PG and PAMAM 4G have been determined. The immunogenicity of the obtained vaccine constructs was investigated in BALB/c mice. Results: It was shown that packaging of DNA vaccine constructs both in the PG envelope and the PAMAM 4G envelope results in an increase in their immunogenicity as compared with the group of mice immunized with the of vector plasmid pcDNA3.1 (a negative control). The highest T-cell responses were shown in mice immunized with complexes of DNA vaccines with PG and these responses significantly exceeded those in the groups of animals immunized with both the combination of naked DNAs and the combination DNAs coated with PAMAM 4G. In the group of animals immunized with complexes of the DNA vaccines with PAMAM 4G, no statistical differences were found in the ability to induce T-cell responses, as compared with the group of mice immunized with the combination of naked DNAs. Conclusions: The PG conjugate can be considered as a promising and safe means to deliver DNA-based vaccines. The use of PAMAM requires further optimization.
Redefining Non-Inferiority in Anamnestic Antibody Responses Using the Mean Increase of Log-Transformed Antibody Titers after Revaccination: Secondary Analysis of a Randomized Controlled Rabies Vaccination Trial
Authors: Overduin Lisanne A. , Soentjens Patrick H. P. , Goeman Jelle J. , Berkowska Magdalena A. , van Dongen Jacques J. M. , Visser Leo G.
Published: December 2020

Non-inferiority in the anamnestic antibody response is conventionally determined by comparing seroconversion rates after revaccination. However, this approach is inadequate in the case of high pre-booster antibody titers. Therefore, we propose an alternative method to determine non-inferiority of booster responses. We used anonymized data from a randomized controlled trial (NCT01388985; EudraCT 2011-001612-62) in 500 adults, comparing a two-visit primary vaccination schedule (two intradermal 0.1 mL rabies vaccine doses on day 0 and 7) with a three-visit schedule (single intradermal 0.1 mL dose on day 0, 7, and 28). Participants were revaccinated intradermally (single dose) 1 to 3 years later. Rabies virus neutralizing antibody titers were measured on day 0 and 7 after revaccination. After log3-transformation of antibody titers, the mean increase in titers after revaccination was compared between schedules. Non-inferiority was defined as the lower bound of the two-sided 95% confidence interval not exceeding −0.369. Four hundred and ten participants fulfilled the inclusion criteria. The mean increase in log3 titer was 2.21 and 2.31 for the two-visit and three-visit schedule, respectively. The difference between these increases was −0.10 [−0.28, 0.08], meeting the non-inferiority criterion. In conclusion, comparing mean increases in log-transformed titers after revaccination appears to be a feasible and more informative method of studying non-inferiority regarding the anamnestic antibody response.
Standardized Methodology for Target Surveillance against African Swine Fever
Authors: Cappai Stefano , Rolesu Sandro , Feliziani Francesco , Desini Pietro , Guberti Vittorio , Loi Federica
Published: December 2020

African swine fever (ASF) remains the most serious pig infectious disease, and its persistence in domestic pigs and wild boar (WB) is a threat for the global industry. The surveillance of WB plays a central role in controlling the disease and rapidly detecting new cases. As we are close to eradicating ASF, the need to find any possible pockets of infection is even more important. In this context, passive surveillance is the method of choice for effective surveillance in WB. Considering the time and economic resources related to passive surveillance, to prioritize these activities, we developed a standardized methodology able to identify areas where WB surveillance should be focused on. Using GIS-technology, we divided a specific Sardinian infected area into 1 km2 grids (a total of 3953 grids). Variables related to WB density, ASF cases during the last three years, sex and age of animals, and the type of land were associated with each grid. Epidemiological models were used to identify the areas with both a lack of information and an high risk of hidden ASFV persistence. The results led to the creation of a graphic tool providing specific indications about areas where surveillance should be a priority.
НА ПУТИ К СОЗДАНИЮ ТЕОРИИ КОГНИТИВНОГО СИНТАКСИСА
Authors: Скоробогатова Елена Александровна
Published: December 2020

Рецензия на монографию: Палатовська О.В. Складне речення в когнітивно-дискурсивному аспекті: Монографія. Київ: ПП «Фірма “Гранмна”», 2019. 400 с.

Исследования в области когнитивной грамматики пополнились новой интересной работой, которая, с одной стороны, продолжает лучшие традиции харьковской школы синтаксиса, а с другой, – служит блестящим примером современного взгляда на природу языка, в частности, на когнитивную сущность и дискурсивный потенциал единиц синтаксиса. Еще Д.Н. Овсянико-Куликовский утверждал, что язык следует изучать и описывать «с начала – то есть с синтаксиса», однако когнитивные исследования последних десятилетий рассматривают в объяснительном ключе в основном лексические явления, тогда как структура и семантика синтаксических конструкций русского языка, так подробно описанная синтаксистами старшего поколения, в когнитивном ракурсе до сих пор была представлена фрагментарно. Рецензируемая монография удачно заполняет существующий пробел.