bluuebunny/crossref_metadata_2025 huggingface dataset:
Profiling of Metabolomic Changes in Plasma and Urine of Pigs Caused by Illegal Administration of Testosterone Esters
Authors: Stastny Kamil , Putecova Kristina , Leva Lenka , Franek Milan , Dvorak Petr , Faldyna Martin
Published: July 2020

The use of anabolic steroid hormones as growth promoters in feed for farm animals has been banned in the European Union since 1988 on the basis of Council Directive 96/22/EC. However, there is still ongoing monitoring and reporting of positive findings of these banned substances in EU countries. The aim of this work was to investigate the efficacy and discriminatory ability of metabolic fingerprinting after the administration of 17β-testosterone esters to pigs. Plasma and urine samples were chromatographically separated on a Hypersil Gold C18 column. High resolution mass spectrometry metabolomic fingerprints were analysed on a hybrid mass spectrometer Q-Exactive. Three independent multivariate statistical methods, namely principal component analysis, clustre analysis, and orthogonal partial least squares discriminant analysis showed significant differences between the treated and control groups of pigs even 14 days after the administration of the hormonal drug. Plasma samples were also analysed by a conventional quantitative analysis using liquid chromatography with tandem mass spectrometry and a pharmacokinetic curve was constructed based on the results. In this case, no testosterone residue was detected 14 days after the administration. The results clearly showed that a metabolomics approach can be a useful and effective tool for the detection and monitoring of banned anabolic steroids used illegally in pig fattening.
Vitreous Tesserae from the Four Seasons Mosaic of the S. Aloe Quarter in Vibo Valentia–Calabria, Italy: A Chemical Characterization
Authors: Fiorenza Elia , Rovella Natalia , D’Andrea Maria , Musella Marianna , Sudano Fabrizio , Taliano Grasso Armando , Barca Donatella
Published: July 2020

This work reports the results of the archaeometrical investigation performed on twenty glass tesserae collected in 2018, during the restoration of the Four Seasons mosaic, which dates between the second and the third century AD, in the archaeological area of the S. Aloe quarter in Vibo Valentia (Calabria, Italy). The coloured glass tesserae were analysed through a micro-analytical approach using an Electron Probe Micro Analyser with Wavelength-Dispersive Spectroscopy (EPMA-WDS) and Laser Ablation with Inductively Coupled Plasma Mass Spectrometry (LA-ICP-MS). The aims of the study were (1) the determination of the chemical composition and the technology of glass mosaic tesserae production; (2) the individuation of colouring and opacifying agents used for the production of the glass tesserae. The glasses show the typical soda–lime–silica composition. EPMA-WDS results prove the use of Sn–Pb antimonates to create yellow glass, and of cuprite to obtain the red colour. Copper and cobalt were employed in both green and blue glasses to produce different shades of colour (grey, tints of green, dark and light blue).
Organically Templated Layered Uranyl Molybdate [C3H9NH+]4[(UO2)3(MoO4)5] Structurally Based on Mineral-Related Modular Units
Authors: Nazarchuk Evgeny , Charkin Dmitri , Siidra Oleg , Kalmykov Stepan
Published: July 2020

A new organically templated uranyl molybdate [C3H9NH+]4[(UO2)3(MoO4)5] was prepared by a hydrothermal method at 220 °C. The compound is monoclinic, Сс, a = 16.768(6), b = 20.553(8), c = 11.897(4) Å, β = 108.195(7), V = 3895(2) Å3, R1 = 0.05. The crystal structure is based upon [(UO2)3(MoO4)5]4− uranyl molybdate layers. The isopropylammonium cations are located in the interlayer. The layers in the structure of [C3H9NH+]4[(UO2)3(MoO4)5] are considered as modular architectures. Topological analysis of layers with UO2:TO4 ratio of 3:5 (TVI = S, Cr, Se, Mo) was performed. Modular description is employed to elucidate the relationships between different structural topologies of [(UO2)3(TO4)5]4− layers and inorganic uranyl-based nanotubules. The possible existence of uranyl molybdate nanotubules is discussed.
Parametric Optimization in Rougher Flotation Performance of a Sulfidized Mixed Copper Ore
Authors: Azizi Asghar , Masdarian Mojtaba , Hassanzadeh Ahmad , Bahri Zahra , Niedoba Tomasz , Surowiak Agnieszka
Published: July 2020

The dominant challenge of current copper beneficiation plants is the low recoverability of oxide copper-bearing minerals associated with sulfide type ones. Furthermore, applying commonly used conventional methodologies does not allow the interactional effects of critical parameters in the flotation processes to be investigated, which is mostly overlooked in the literature. To tackle this issue, the present paper aimed at characterizing the behavior of five key effective factors and their interactions in a sulfidized copper ore. In this context, dosage of collector (sodium di-ethydithiophosphate, 60–100 g/t), depressant (sodium silicate, 80–120 g/t) and frother (methyl isobutyl carbinol (MIBC), 6–10 g/t), pulp pH (7–11) and agitation rate (900–1300 rpm) were examined and statistically analyzed using response surface methodology. Flotation experiments were conducted in a Denver type agitated flotation cell at the rougher stage. The experimental results showed that increasing the pH (from 8 to 10) at low agitation rate (1000 rpm) enhanced the recovery from 80.36% to 85.22%, while at high agitation rate (1200 rpm), a slight declination occurred in the recovery. Meanwhile, increasing the collector dosage at a lower frother value (7 g/t), caused a reduction of about 4.44% in copper recovery owing to the interactions between factors, whereas at a higher frother level (9 g/t), the recovery was almost unchanged. The optimization process was also performed using the goal function approach, and maximum copper recovery of 92.75% was obtained using ~70 g/t collector, 110 g/t depressant, 7 g/t frother, pulp pH of 10 and 1000 rpm agitation rate.
Relationship between Textural Parameters of Lamellar Products Obtained by Acid Activation of Pure and Commercial Vermiculites and Their Iron and Water Content
Authors: Marcos Celia , Adawy Alaa , Rodríguez Irene
Published: July 2020

The relationship between textural parameters (specific superficial area (SBET) and porosity (Vp)) of lamellar products obtained from HNO3-activated vermiculites and their iron and water content has been established. Two commercial vermiculites, one thermoexfoliated commercial vermiculite, and one pure vermiculite were nitric-acid-treated at 4 and 8 M concentrations for 1, 3, and 7 days. Untreated and treated samples were characterized with X-ray diffraction (XRD), scanning electron microscopy (SEM), high-resolution transmission electron microscopy (HRTEM), and N2 physisorption analysis. The untreated vermiculites showed a direct relationship between their iron content and the values of SBET, Vp, and pore size; an inverse relationship was observed in the case of the treated samples. The iron content may prevent further leaching of cations but not water loss, therefore forming lamellar products with lower SBET and Vp values. The SBET and Vp values of the studied thermoexfoliated sample were higher than those of the starting sample. The SBET and Vp values of the activated thermoexfoliated sample were lower than those of the activated non-thermoexfoliated sample.
Confined Bed Breakage of Fine Iron Ore Concentrates
Authors: Campos Túlio M. , Bueno Gilvandro , Tavares Luís Marcelo
Published: July 2020

High-pressure grinding rolls (HPGR) have gained great popularity in the mining industry in the last 25 years or so. One of the first successful applications of the technology has been in iron ore pressing prior to pelletization. Piston-and-die tests can provide good insights on the material response in an HPGR. This work analyzed confined bed breakage of four iron ore concentrates under different conditions. Saturation in breakage of particles contained in the top size in the tests was observed to occur at specific energies of about 2 kWh/t, whereas full saturation in breakage, with no additional increase in specific surface area of the material, occurred at energies above about 6 kWh/t. An expression was proposed to characterize the propensity of a material to break under confined bed conditions. The phenomenology involved in confined bed breakage of such materials was then analyzed in light of the results.
Dynamic Effects of Laser Action on Quasi-Two-Dimensional Dusty Plasma Systems of Charged Particles
Authors: Vasiliev Mikhail M. , Petrov Oleg F. , Alekseevskaya Anastasiya A. , Ivanov Alexander S. , Vasilieva Elena V.
Published: July 2020

We present the results of an experimental study of the behavior of a colloidal plasma system formed by copper-coated and uncoated polymer particles under the action of laser irradiation. A comparative study of particle velocity distribution profiles depending on the power of the pushing laser was conducted. In the case of uncoated melamine-formaldehyde (MF) particles, we observed the well-known action of light pressure, causing shear stress in the colloidal plasma structure and leading to the occurrence of a laminar flow within the affected area. For the copper-coated MF particles, we revealed some additional patterns of behavior for the dust particles, i.e., kinetic temperature growth due to laser radiation absorption by the copper coating, as well as the appearance of chaotic particle motion. We believe that this happens due to the existence of defects in the coating, causing asymmetric heating of the particles, which in turn leads to chaotic deviations of the photophoretic force pushing the particles in different directions.
Five-Coordinated Geometries from Molecular Structures to Solutions in Copper(II) Complexes Generated from Polydentate-N-Donor Ligands and Pseudohalides
Authors: Mautner Franz A. , Fischer Roland C. , Torvisco Ana , Henary Maher M. , Louka Febee R. , Massoud Salah S. , Salem Nahed M. H.
Published: July 2020

A novel series of mononuclear five-coordinated pseudohalido-Cu(II) complexes displaying distorted square bipyramidal: [Cu(L1)(NCS)2] (1), [Cu(L2)(NCS)2] (2) and [Cu(L3)(NCS)]ClO4 (5) as well as distorted trigonal bipyramidal: [Cu(isp3tren)(N3)]ClO4 (3), [Cu(isp3tren)(dca)]ClO4 (4) and [Cu(tedmpza)(dca)]ClO4·0.67H2O (6) geometries had been synthesized and structurally characterized using X-ray single crystal crystallography, elemental microanalysis, IR and UV-vis spectroscopy, and molar conductivity measurements. Different N-donor amine skeletons including tridentate: L1 = [(2-pyridyl)-2-ethyl)-(3,4-dimethoxy)-2-methylpyridyl]methylamine and L2 = [(2-pyridyl)-2-ethyl)-(3,5-dimethyl-4-methoxy)-2-methyl-pyridyl]methylamine, and tetradentate: L3 = bis(2-ethyl-di(3,5-dimethyl-1H-pyrazol-1-yl)-[2-(3,4-dimethoxy-pyridylmethyl)]amine, tedmpza = tris[(2-(3,5-dimethyl-1H-pyrazol-1-yl)ethyl]amine and isp3tren = tris[(2-isopropylamino)ethyl)]amine ligands were employed. Molecular structural parameters such as nature of coligand, its chelate ring size and steric environment incorporated into its skeleton, which lead to adopting one of the two limiting geometries in these complexes and other reported compounds are analyzed and correlated to their assigned geometries in solutions. Similar analysis were extended to other five-coordinated halido-Cu(II) complexes.
Synthesis and Characterization of New Organic Dyes Containing the Indigo Core
Authors: Franchi Daniele , Calamante Massimo , Coppola Carmen , Mordini Alessandro , Reginato Gianna , Sinicropi Adalgisa , Zani Lorenzo
Published: July 2020

A new series of symmetrical organic dyes containing an indigo central core decorated with different electron donor groups have been prepared, starting from Tyrian Purple and using the Pd-catalyzed Stille-Migita coupling process. The effect of substituents on the spectroscopic properties of the dyes has been investigated theoretically and experimentally. In general, all dyes presented intense light absorption bands, both in the blue and red regions of the visible spectrum, conferring them a bright green color in solution. Using the same approach, an asymmetrically substituted D–A-π–A green dye, bearing a triarylamine electron donor and the cyanoacrylate acceptor/anchoring group, has been synthesized for the first time and fully characterized, confirming that spectroscopic and electrochemical properties are consistent with a possible application in dye-sensitized solar cells (DSSC).
Hydrogels as Durable Anti-Icing Coatings Inhibit and Delay Ice Nucleation
Authors: Huang Beili , Jiang Shanshan , Diao Yunhe , Liu Xuying , Liu Wentao , Chen Jinzhou , Yang Huige
Published: July 2020

The accumulation of ice on surfaces brings dangerous and costly problems to our daily life. Thus, it would be desirable to design anti-icing coatings for various surfaces. We report a durable anti-icing coating based on mussel-inspired chemistry, which is enabled via fabricating a liquid water layer, achieved by modifying solid substrates with the highly water absorbing property of sodium alginate. Dopamine, the main component of the mussel adhesive protein, is introduced to anchor the sodium alginate in order to render the coating applicable to all types of solid surfaces. Simultaneously, it serves as the cross-linking agent for sodium alginate; thus, the cross-linking degree of the coatings could be easily varied. The non-freezable and freezable water in the coatings with different cross-link degrees all remain liquid-like at subzero conditions and synergistically fulfill the aim of decreasing the temperature of ice nucleation. These anti-icing coatings display excellent stability even under harsh conditions. Furthermore, these coatings can be applied to almost all types of solid surfaces and have great promise in practical applications.
Metabolomics Reveals the Alteration of Metabolic Pathway by Alpha-Melanocyte-Stimulating Hormone in B16F10 Melanoma Cells
Authors: Seo Seung-Ho , Jo Jae Kwon , Kim Eun-Ju , Park Seong-Eun , Shin Seo Yeon , Park Kyung Mok , Son Hong-Seok
Published: July 2020

The purpose of this study was to understand the changes of metabolic pathway induced by alpha-melanocyte-stimulating hormone (α-MSH) in B16F10 melanoma cells in an untargeted metabolomics approach. Cells were treated with 100 nM of α-MSH and then incubated for 48 h. α-MSH increased tyrosinase activity and melanin content by 56.5 and 61.7%, respectively, compared to untreated cells after 48 h of cultivation. The clear separation between groups was observed in the principal component analysis score plot, indicating that the levels of metabolites of melanoma cells were altered by treatment with α-MSH. Metabolic pathways affected by α-MSH were involved in some amino acid metabolisms. The increased levels of fumaric acid, malic acid, oxaloacetic acid and citric acid related to the citric acid cycle pathway after α-MSH treatment suggested enhanced energy metabolism. Metabolic pathways altered by α-MSH treatment can provide useful information to develop new skin pigmentation inhibitors or anti-obesity drugs.
Transport and Electrochemical Interface Properties of Ionomers in Low-Pt Loading Catalyst Layers: Effect of Ionomer Equivalent Weight and Relative Humidity
Authors: Poojary Sushmit , Islam Muhammad Naoshad , Shrivastava Udit N. , Roberts Edward P. L. , Karan Kunal
Published: July 2020

Catalyst layer (CL) ionomers control several transport and interfacial phenomena including long-range transport of protons, local transport of oxygen to Pt catalyst, effective utilization of Pt catalyst, electrochemical reaction kinetics and double-layer capacitance. In this work, the variation of these properties, as a function of humidity, for CLs made with two ionomers differing in side-chain length and equivalent weight, Nafion-1100 and Aquivion-825, was investigated. This is the first study to examine humidity-dependent oxygen reduction reaction (ORR) kinetics in-situ for CLs with different ionomers. A significant finding is the observation of higher ORR kinetic activity (A/cm2Pt) for the Aquivion-825 CL than for the Nafion-1100 CL. This is attributed to differences in the interfacial protonic concentrations at Pt/ionomer interface in the two CLs. The differences in Pt/ionomer interface is also noted in a higher local oxygen transport resistance for Aquivion-825 CLs compared to Nafion-1100 CLs, consistent with stronger interaction between ionomer and Pt for ionomer with more acid groups. Similar dependency on Pt utilization (ratio of electrochemically active area at any relative humidity (RH) to that at 100% RH) as a function of RH is observed for the two CLs. As expected, strong influence of humidity on proton conduction is observed. Amongst the two, the CL with high equivalent weight ionomer (Nafion-1100) exhibits higher conduction.
Evaluation of the Immunosafety of Cucurbit[n]uril on Peripheral Blood Mononuclear Cells In Vitro
Authors: Pashkina Ekaterina , Aktanova Alina , Blinova Elena , Mirzaeva Irina , Kovalenko Ekaterina , Knauer Nadezhda , Ermakov Aleksandr , Kozlov Vladimir
Published: July 2020

Cucurbiturils (CB[n]s) are nanoscale macrocyclic compounds capable of encapsulating a molecule or part of a molecule by forming host–guest complexes. Integration of drugs with CB[n] is used for the following purposes: controlling clearance; protection of the drug from biodegradation; targeted delivery to specific organs, tissues, or cells; reduction of toxicity; and improving solubility. One of the major problems encountered in the application of new drug delivery systems is lack of knowledge of their biological properties. CB[n], unlike many other often toxic nanoparticles, has extremely low toxicity, even at high doses. However, many aspects of the biological actions of these nanoscale cavitands remain unclear, including the immunotropic properties. In this study, we investigated the immunotoxicity and immunomodulation properties of CB[n]. It was found that CB[7] and CB[6] did not decrease the viability of mononuclear cells at all tested concentrations from 0.1–1 mM. Overall, the results indicated an immunomodulatory effect of different concentrations of CB[n]. In the case of a longer cultivation time, CB[n] had an immunostimulating effect, which was indicated by an enhancement of the proliferative activity of cells and increased expression of HLA-DR on lymphocytes.
Resistance to Chemical Attack of Hybrid Fly Ash-Based Alkali-Activated Concretes
Authors: Valencia-Saavedra William G. , Mejía de Gutiérrez Ruby
Published: July 2020

The environmental impacts related to Portland cement production in terms of energy consumption, the massive use of natural resources and CO2 emissions have led to the search for alternative cementitious materials. Among these materials, alkali-activated cements based on fly ash (FA) have been considered for concrete production with greater sustainability. In the present article, the chemical durability properties (resistance to sulphates, chloride permeability, and resistance to carbonation) of a hybrid alkali-activated concrete based on fly ash–ordinary Portland cement (FA/OPC) with proportions of 80%/20% were evaluated. It is noted that the FA was a low-quality pozzolan with a high unburned carbon content (20.67%). The results indicated that FA/OPC concrete had good durability with respect to the OPC concrete, with 95% less expansion in the presence of sodium sulphate and a 2% strength loss at 1100 days, compared with the 56% strength loss of the OPC concrete. In addition, FA/OPC showed lower chloride permeability. On the contrary, the FA/OPC was more susceptible to carbonation. However, the residual compressive strength was 23 MPa at 360 days of CO2 exposure. Based on the results, FA/OPC, using this type of FA, can be used as a replacement for OPC in the presence of these aggressive agents in the service environment.
Comparison of Sin-QuEChERS Nano and d-SPE Methods for Pesticide Multi-Residues in Lettuce and Chinese Chives
Authors: Li Yanjie , An Quanshun , Zhang Changpeng , Pan Canping , Zhang Zhiheng
Published: July 2020

In this study, a new rapid cleanup method was developed for the analysis of 111 pesticide multi-residues in lettuce and Chinese chives by GC–MS/MS and LC–MS/MS. QuEChERS (quick, easy, cheap, effective, rugged and safe)-based sample extraction was used to obtain the extracts, and the cleanup procedure was carried out using a Sin-QuEChERS nano cartridge. Comparison of the cleanup effects, limits of quantification and limits of detection, recoveries, precision and matrix effects (MEs) between the Sin-QuEChERS nano method and the classical dispersive solid phase extraction (d-SPE) method were performed. When spiked at 10 and 100 μg/kg, the number of pesticides with recoveries between 90% to 110% and relative standard deviations < 15% were greater when using the Sin-QuEChERS nano method. The MEs of Sin-QuEChERS nano and d-SPE methods ranged between 0.72 to 3.41 and 0.63 to 3.56, respectively. The results verified that the Sin-QuEChERS nano method was significantly more effective at removing pigments and more convenient than the d-SPE method. The developed method with the Sin-QuEChERS nano cleanup procedure was applied successfully to determine pesticide residues in market samples.
Diastereoisomerically Pure, (S)-O-1,2-O-isopropyli dene-(5-O-α-d-glucofuranosyl) t-butanesulfinate: Synthesis, Crystal Structure, Absolute Configuration and Reactivity
Authors: Bujnicki Bogdan , Błaszczyk Jarosław , Chmielewski Marek , Drabowicz Józef
Published: July 2020

The reaction of t-butylmagnesium chlorides with diastereomerically pure (R)-1,2-O-isopropylidene-3,5-O-sulfinyl-α-d-glucofuranose (R)-4 was found to be stopped at the stage of the corresponding, diastereoisomerically pure 1,2-O-isopropylidene-(5-O-α-d-glucofuranosyl) t-butanesulfinate (S)-10 for which the crystal structure and the (S)-absolute configuration was determined by X-ray crystallography. Comparison of the absolute configurations of the starting sulfite (R)-4, and t-butanesulfinate (S)-10 (which crystallizes in the orthorhombic system, space group P212121, with the single compound molecule present in the asymmetric unit), clearly indicates that the reaction of nucleophilic substitution at the stereogenic sulfur atom in the sulfite (R)-4 occurs with the full inversion of configuration via the trigonal bipyramidal sulfurane intermediate 4c in which both the entering and leaving groups are located in apical positions.
Probing the Proton-Loading Site of Cytochrome C Oxidase Using Time-Resolved Fourier Transform Infrared Spectroscopy
Authors: Gorbikova Elena , Samsonov Sergey A. , Kalendar Ruslan
Published: July 2020

Crystal structure analyses at atomic resolution and FTIR spectroscopic studies of cytochrome c oxidase have yet not revealed protonation or deprotonation of key sites of proton transfer in a time-resolved mode. Here, a sensitive technique to detect protolytic transitions is employed. In this work, probing a proton-loading site of cytochrome c oxidase from Paracoccus denitrificans with time-resolved Fourier transform infrared spectroscopy is presented for the first time. For this purpose, variants with single-site mutations of N131V, D124N, and E278Q, the key residues in the D-channel, were studied. The reaction of mutated CcO enzymes with oxygen was monitored and analyzed. Seven infrared bands in the “fast” kinetic spectra were found based on the following three requirements: (1) they are present in the “fast” phases of N131V and D124N mutants, (2) they have reciprocal counterparts in the “slow” kinetic spectra in these mutants, and (3) they are absent in “fast” kinetic spectra of the E278Q mutant. Moreover, the double-difference spectra between the first two mutants and E278Q revealed more IR bands that may belong to the proton-loading site protolytic transitions. From these results, it is assumed that several polar residues and/or water molecule cluster(s) share a proton as a proton-loading site. This site can be propionate itself (holding only a fraction of H+), His403, and/or water cluster(s).
Amburana cearensis: Pharmacological and Neuroprotective Effects of Its Compounds
Authors: Silva Juliana Helena Castro e , Ferreira Rafael Short , Pereira Erica Patricia , Braga-de-Souza Suzana , Almeida Monique Marylin Alves de , Santos Cleonice Creusa dos , Butt Arthur Morgan , Caiazzo Elisabetta , Capasso Raffaele , Silva Victor Diogenes Amaral da , Costa Silvia Lima
Published: July 2020

Amburana cearensis A.C. Smith is an endemic tree from Northeastern Brazil used in folk medicine as teas, decocts and syrups for the treatment of various respiratory and inflammatory diseases, since therapeutic properties have been attributed to compounds from its stem bark and seeds. Numerous pharmacological properties of semi-purified extracts and isolated compounds from A. cearensis have been described in several biological systems, ranging from antimicrobial to anti-inflammatory effects. Some of these activities are attributed to coumarins and phenolic compounds, the major compounds present in A. cearensis seed extracts. Multiple lines of research demonstrate these compounds reduce oxidative stress, inflammation and neuronal death induced by glutamate excitotoxicity, events central to most neuropathologies, including Alzheimer’s disease (AD) and Parkinson’s Disease (PD). This review focuses on the botanical aspects, folk medicine use, biological effects and pharmacological activities of A. cearensis compounds and their potential as novel non-toxic drugs for the treatment of neurodegenerative diseases.
Exfoliated Clay Decorated with Magnetic Iron Nanoparticles for Crystal Violet Adsorption: Modeling and Physicochemical Interpretation
Authors: Barakat Mohamed Abou Elfetouh , Kumar Rajeev , Seliem Moaaz Korany , Selim Ali Qurany , Mobarak Mohamed , Anastopoulos Ioannis , Giannakoudakis Dimitrios , Barczak Mariusz , Bonilla-Petriciolet Adrián , Mohamed Essam Abdelrahman
Published: July 2020

Surfactant–modified exfoliated Fayum clay (CTAB–EC) obtained after chemical treatment with a CTAB/H2O2 solution was further decorated with magnetic Fe3O4 nanoparticles (MNP). The final nanocomposite (MNP/CTAB–EC) was characterized by XRD, SEM, FTIR, TEM and its adsorptive capability against a model cationic dye, crystal violet (CV), was evaluated. A comparison of the adsorption performance of the raw clay and its modified counterparts using H2O2, CTAB, CTAB/H2O2 or MNP indicated that the adsorption capacity of MNP/CTAB–EC was the highest for CV removal at pH 8.0. The pseudo‒second order for the kinetics and Freundlich model for adsorption equilibrium fitted well the CV removal experimental data at all tested temperatures (25, 40 and 55 °C). The enhancement of the Langmuir adsorption capacity from 447.1 to 499.4 mg g−1 with increasing the temperature from 25 to 55 °C revealed an endothermic nature of the removal process. The interactions between CV and MNP/CTAB–EC were interpreted using advanced statistical physics models (ASPM) in order to elucidate the adsorption mechanism. Multilayer model fitted the adsorption process and therefore, the steric and energetic factors that impacted the CV adsorption were also interpreted using this model. The aggregated number of CV molecules per MNP/CTAB–EC active site ( n ) was more than unity at all temperatures, representing thus a vertical adsorption orientation and a multi‒interactions mechanism. It was determined that the increase of CV uptake with temperature was mainly controlled by the increase of the number of active sites (NM). Calculated adsorption energies (ΔE) revealed that CV removal was an endothermic and a physisorption process (ΔE < 40 kJ mol −1). MNP/CTAB–EC was magnetically separated, regenerated by NaOH, and reused without significant decrease in its adsorption efficiency, supporting a prosperity of its utilization as an effective adsorbent against hazardous dyes from wastewaters.
New Relevant Descriptor of Linear QNAR Models for Toxicity Assessment of Silver Nanoparticles
Authors: Kudrinskiy Alexey , Zherebin Pavel , Gusev Alexander , Shapoval Olga , Pyee Jaeho , Lisichkin Georgy , Krutyakov Yurii
Published: July 2020

The use of silver nanoparticles (NPs) in medical, industrial and agricultural fields is becoming more widespread every year. This leads to an increasing number of experimental toxicological and microbiological studies of silver NPs aimed at establishing the risk–benefit ratio for their application. The following key parameters affecting the biological activity of silver dispersions are traditionally taken into consideration: mean diameter of NPs, surface potential of NPs and equilibrium concentration of Ag+. These characteristics are mainly predetermined by the chemical nature of the capping agent used for stabilization. However, the extent to which they influence the biological activity and the toxicity of silver NPs varies greatly. In this work, dispersions of silver NPs stabilized with a wide array of substances of different chemical nature were used for quantitative evaluation of whether the various measurable properties of silver NPs fit as descriptors of linear QNAR (quantitative nanostructure–activity relationship) models for silver NP toxicity evaluation with respect to a model eukaryotic microorganism—Saccharomyces cerevisiae yeast cells. It was shown that among the factors that determine silver NP toxicity, the charge of particles, their colloidal stability and the ability to generate Ag+ ions carry more importance than the descriptors related to the particle size. A significant synergistic effect between the ζ-potential and the colloidal stability of silver NPs on their toxicity was also discovered. Following this, a new descriptor has been proposed for the integral characterization of the silver dispersion colloidal stability. According to the obtained data, it can be considered applicable for building QNAR models of higher efficacy. The validity testing of the proposed model for theoretical prediction of silver NP toxicity using a wide range of living organisms has shown that this new descriptor correlates with toxicity much better compared to most traditionally used descriptors. Consequently, it seems promising in terms of being used not only in situations involving the rather narrow array of the objects tested, but also for the construction of silver NP toxicity models with respect to other living organisms.
Biological Assessment of Laser-Synthesized Silicon Nanoparticles Effect in Two-Photon Photodynamic Therapy on Breast Cancer MCF-7 Cells
Authors: Al-Kattan Ahmed , M. A. Ali Lamiaa , Daurat Morgane , Mattana Elodie , Gary-Bobo Magali
Published: July 2020

Driven by their distinctive physiological activities, biological properties and unique theranostic modalities, silicon nanoparticles (SiNPs) are one of the promising materials for the development of novel multifunctional nanoplatforms for biomedical applications. In this work, we assessed the possibility to use laser-synthesized Si NPs as photosensitizers in two-photon excited photodynamic therapy (TPE-PDT) modality. Herein, we used an easy strategy to synthesize ultraclean and monodispersed SiNPs using laser ablation and fragmentation sequences of silicon wafer in aqueous solution, which prevent any specific purification step. Structural analysis revealed the spherical shape of the nanoparticles with a narrow size distribution centered at the mean size diameter of 62 nm ± 0.42 nm, while the negative surface charge of −40 ± 0.3 mV ensured a great stability without sedimentation over a long period of time. In vitro studies on human cancer cell lines (breast and liver) and healthy cells revealed their low cytotoxicity without any light stimulus and their therapeutic potential under TPE-PDT mode at 900 nm with a promising cell death of 45% in case of MCF-7 breast cancer cells, as a consequence of intracellular reactive oxygen species release. Their luminescence emission inside the cells was clearly observed at UV-Vis region. Compared to Si nanoparticles synthesized via chemical routes, which are often linked to additional modules with photochemical and photobiological properties to boost photodynamic effect, laser-synthesized SiNPs exhibit promising intrinsic therapeutic and imaging properties to develop advanced strategy in nanomedicine field.
Laser-Ablative Synthesis of Stable Aqueous Solutions of Elemental Bismuth Nanoparticles for Multimodal Theranostic Applications
Authors: Bulmahn Julia C. , Tikhonowski Gleb , Popov Anton A. , Kuzmin Andrey , Klimentov Sergey M. , Kabashin Andrei V. , Prasad Paras N.
Published: July 2020

Elemental bismuth (Bi) nanoparticles (NPs), with the high atomic density of the Bi nuclei, could serve as efficient targeted agents for cancer treatment, with applications such as contrast agents for computed tomography (CT) imaging, sensitizers for image-guided X-ray radiotherapy, and photothermal therapy. However, the synthesis of elemental Bi NPs suitable for biological applications is difficult using conventional chemical routes. Here, we explore the fabrication of ultrapure Bi-based nanomaterials by femtosecond laser ablation from a solid Bi target in ambient liquids and characterize them by a variety of techniques, including TEM, SEM, XRD, FTIR, Raman, and optical spectroscopy. We found that laser-ablative synthesis using an elemental Bi solid target leads to the formation of spherical Bi NPs having the mean size of 20–50 nm and a low size-dispersion. The NPs prepared in water experience a fast (within a few minutes) conversion into 400–500 nm flake-like nanosheets, composed of bismuth subcarbonates, (BiO)2CO3 and (BiO)4CO3(OH)2, while the NPs prepared in acetone demonstrate high elemental stability. We introduce a procedure to obtain a stable aqueous solution of elemental Bi NPs suitable for biological applications, based on the coating of Bi NPs prepared in acetone with Pluronic® F68 and their subsequent transfer to water. We also show that the laser-synthesized elemental Bi NPs, due to their vanishing band gap, exhibit remarkable absorption in the infrared range, which can be used for the activation of photothermal therapy in the near IR-to-IR window with maximum optical transparency in biological media. Exempt of any toxic synthetic by-products, laser-ablated elemental Bi NPs present a novel appealing nanoplatform for combination image-guided photoradiotherapies.
MCMV Dissemination from Latently-Infected Allografts Following Transplantation into Pre-Tolerized Recipients
Authors: Shah Sahil , DeBerge Matthew , Iovane Andre , Yan Shixian , Qiu Longhui , Wang Jiao-Jing , Kanwar Yashpal S. , Hummel Mary , Zhang Zheng J. , Abecassis Michael M. , Luo Xunrong , Thorp Edward B.
Published: July 2020

Transplantation tolerance is achieved when recipients are unresponsive to donor alloantigen yet mobilize against third-party antigens, including virus. After transplantation, cytomegalovirus (CMV) reactivation in latently-infected transplants reduces allograft viability. To determine if pre-tolerized recipients are resistant to viral dissemination in this setting, we transfused chemically-fixed donor splenocytes (1-ethyl-3- (3′-dimethyl-aminopropyl)-carbo-diimide (ECDI)-treated splenocytes (ECDIsp)) to induce donor antigen tolerance without immunosuppression. In parallel, we implanted donor islet cells to validate operational tolerance. These pre-tolerized recipients were implanted with murine CMV (MCMV) latently-infected donor kidneys (a validated model of CMV latency) to monitor graft inflammation and viral dissemination. Our results indicate that tolerance to donor islets was sustained in recipients after implantation of donor kidneys. In addition, kidney allografts implanted after ECDIsp and islet implantation exhibited low levels of fibrosis and tubulitis. In contrast, kidney cellular and innate immune infiltrates trended higher in the CMV group and exhibited increased markers of CD8+ T cell activation. Tolerance induction was unable to prevent increases in MCMV-specific CD8+ T cells or dissemination of viral IE-1 DNA. Our data suggest that latently-infected allografts are inherently more susceptible to inflammation that is associated with viral dissemination in pre-tolerized recipients. Thus, CMV latently-infected allografts require enhanced strategies to protect allograft integrity and viral spread.
Protection of Piglets with Maternally Derived Antibodies from Sows Inoculated with an Attenuated Live Marker Classical Swine Fever Vaccine (Flc-LOM-BErns)
Authors: Choe SeEun , Shin Jihye , Kim Ki-Sun , Song Sok , Cha Ra Mi , Jung Byung-Il , Hyun Bang-Hun , Park Bong-Kyun , An Dong-Jun
Published: July 2020

Here, we investigated the protective efficacy provided by passive immunity induced by a classical swine fever (Flc-LOM-BErns) vaccine with the newly developed DIVA (Differentiating Infected from Vaccinated Animals) function. Ten pigs (aged 40–60 days) with maternally derived antibodies (MDAs) obtained from sows inoculated with the Flc-LOM-BErns vaccine were challenged with virulent classical swine fever virus (CSFV). Pigs with an MDA titer of 6 log2 induced by the Flc-LOM-BErns vaccine were fully protected against virulent CSFV challenge but not the pigs with an MDA titer under 5 log2. In addition, Flc-LOM-BErns vaccine-derived MDAs successfully differentiated vaccinated pigs by bovine viral diarrhea virus (BVDV) Erns/CSFV Erns antibody detection, functioning as a DIVA.
Leaves of White Beetroot As a New Source of Antioxidant and Anti-Inflammatory Compounds
Authors: Gawlik-Dziki Urszula , Dziki Laura , Anisiewicz Jakub , Habza-Kowalska Ewa , Sikora Małgorzata , Dziki Dariusz
Published: July 2020

The white beetroot cv. Śnieżna Kula is the first betanin-free beetroot registered in the European Union. The aim of this study was to compare the phenolic acids profile and antioxidant capacity of leaves of white (SK) and red (CC) beetroots and red (LC) and white (BL) Swiss chard growing in Poland. LC leaves were the richest source of total phenolics (16.55 mg GAE/g FW) and phenolic acids (1.81 mg/g FW), while the highest content of flavonoids was determined in CC leaves (1.6 mg QE/g FW). The highest antiradical activity was observed for LC, whereas CC extract exhibited the highest chelating power. BL and CC leaf extracts demonstrated high LOX inhibitory potential (EC50 = 53.23 and 56.97 mg FW/mL, respectively). An uncompetitive type of LOX inhibition was obtained for all extracts. SK extracts demonstrated the highest XO inhibitory activity (EC50 = 81.04 mg FW/mL). A noncompetitive type of XO inhibition was obtained in both extracts from red leaves (CC and LC), whereas an uncompetitive mode of inhibition was observed in the case of white leaf (SK and LC) extracts. Thus, it can be assumed that the presence of betanin influences the XO inhibition mechanism.
Characterizing the Core-Shell Architecture of Block Copolymer Nanoparticles with Electron Microscopy: A Multi-Technique Approach
Authors: Tkachenko Vitalii , Vidal Loïc , Josien Ludovic , Schmutz Marc , Poly Julien , Chemtob Abraham
Published: July 2020

Electron microscopy has proved to be a major tool to study the structure of self-assembled amphiphilic block copolymer particles. These specimens, like supramolecular biological structures, are problematic for electron microscopy because of their poor capacity to scatter electrons and their susceptibility to radiation damage and dehydration. Sub-50 nm core-shell spherical particles made up of poly(hydroxyethyl acrylate)–b–poly(styrene) are prepared via polymerization-induced self-assembly (PISA). For their morphological characterization, we discuss the advantages, limitations, and artefacts of TEM with or without staining, cryo-TEM, and SEM. A number of technical points are addressed such as precisely shaping of particle boundaries, resolving the particle shell, differentiating particle core and shell, and the effect of sample drying and staining. TEM without staining and cryo-TEM largely evaluate the core diameter. Negative staining TEM is more efficient than positive staining TEM to preserve native structure and to visualize the entire particle volume. However, no technique allows for a satisfactory imaging of both core and shell regions. The presence of long protruding chains is manifested by patched structure in cryo-TEM and a significant edge effect in SEM. This manuscript provides a basis for polymer chemists to develop their own specimen preparations and to tackle the interpretation of challenging systems.
Quantum Beams Applying to Innovative Industrial Materials
Authors: Maekawa Yasunari , Washio Masakazu
Published: July 2020

Welcome to this Special Issue of Quantum Beam Science entitled “Quantum Beams Applying to Innovative Industrial Materials” [...]
Generating a Spatio-Temporal Complete 30 m Leaf Area Index from Field and Remote Sensing Data
Authors: Zhou Hongmin , Wang Changjing , Zhang Guodong , Xue Huazhu , Wang Jingdi , Wan Huawei
Published: July 2020

The leaf area index (LAI) is an important parameter for vegetation monitoring and land surface ecosystem research. Although a variety of LAI products have been generated, the moderate to coarse spatial resolution and low temporal resolution of these products are insufficient for regional-scale analysis. In this study, a modified ensemble Kalman filter model (MEnKF) was proposed to generate spatio-temporal complete 30 m LAI data. High-quality, filtered historical Moderate-resolution Imaging Spectroradiometer (MODIS) LAI data were used to obtain the LAI background, and an LAI temporal dynamic model was constructed based on it. An improved back-propagation (BP) neural network based on a simulated annealing algorithm (SA-BP) was constructed with paired Landsat surface reflectance data and field LAI data to generate a 30 m LAI. The MEnKF was used to estimate the spatio-temporal complete LAI beginning from the LAI peak value position where Landsat observations were available. The spatio-temporal 30 m LAI was estimated in farmland (Pshenichne), grassland (Zhangbei), and woodland (Genhe) sites. The results indicate that the MEnKF-estimated LAI is consistent with the field measurements for all sites (the coefficient of determination ( R 2 ) = 0.70; root mean squared error (RMSE) = 0.40) and is better than that of the conventional sequence data assimilation algorithm ( R 2 = 0.40; RMSE = 0.78). The regional LAI captures the vegetation growth pattern and is consistent with the Landsat LAI, with an R 2 larger than 0.65 and an RMSE less than 0.51. The proposed MEnKF algorithm, which effectively avoids error accumulation in the data assimilation scheme, is an efficient method for spatio-temporal complete 30 m LAI estimation.
Traceable Radiometry Underpinning Terrestrial- and Helio-Studies (TRUTHS): An Element of a Space-Based Climate and Calibration Observatory
Authors: Fox Nigel , Green Paul
Published: July 2020

The Earth’s climate is undoubtedly changing; however, the time scale, consequences, and causal attribution remain the subject of significant debate and uncertainty. Detection of subtle indicators from a background of natural variability requires measurements over a time-base of decades. This places severe demands on the instrumentation used, requiring measurements of sufficient accuracy and sensitivity that can allow reliable judgements to be made decades apart. The International System of Units (SI) was developed to address such requirements, providing a reference framework tied to invariant constants of nature. However, ensuring and maintaining SI traceability of sufficient accuracy in instruments orbiting the Earth presents a significant new challenge to the Earth Observation and metrology communities. This paper describes a new satellite mission, called Traceable Radiometry Underpinning Terrestrial- and Helio- Studies (TRUTHS), which enables, for the first time, high-accuracy SI traceability to be established in orbit. The direct use of a ‘primary standard’ and replication of the terrestrial traceability chain extends the SI into space, in effect realizing a ‘metrology laboratory in space’ providing and enabling SI-traceable measurements of unequivocal accuracy in the solar reflective domain—an enabling element of an international space-based climate observing system. TRUTHS will not only provide a benchmark of the radiation state of the planet (incoming and outgoing) from which to compare change in the shortest time possible, but also facilitate an upgrade in performance of the Earth Observing system as a whole, through ‘in-orbit’ reference calibration.
Effects of CO2 Changes on Hyperspectral Infrared Radiances and Its Implications on Atmospheric Temperature Profile Retrieval and Data Assimilation in NWP
Authors: Di Di , Xue Yunheng , Li Jun , Bai Wenguang , Zhang Peng
Published: July 2020

Although atmospheric CO2 is a trace gas, it has seasonal variations and has increased over the last decade. Its seasonal variation and increase have substantial radiative effects on hyperspectral infrared (IR) radiance calculations in both longwave (LW) and shortwave (SW) CO2 absorption spectral regions that are widely used for weather and climate applications. The effects depend on the spectral coverage and spectral resolution. The radiative effect caused by the increase of CO2 has been calculated to be greater than 0.5 K within 5 years, whereas a radiative effect of 0.1–0.5 K is introduced by the seasonal variation in some CO2 absorption spectral regions. It is important to take into account the increasing trend and seasonal variation of CO2 in retrieving the atmospheric temperature profile from hyperspectral IR radiances and in the radiance assimilation in numerical weather prediction (NWP) models. The simulation further indicates that it is very difficult to separate atmospheric temperature and CO2 information from hyperspectral IR sounder radiances because the atmospheric temperature signal is much stronger than that of CO2 in the CO2 absorption IR spectral regions.
Electric Current Waveform of the Injector as a Source of Diagnostic Information
Authors: Więcławski Krzysztof , Mączak Jędrzej , Szczurowski Krzysztof
Published: July 2020

The article discusses the method of evaluation of the fuel injector operation based on the observation of the electric current parameters, which were measured with a current transducer using the Hall effect, during the dosing process. This method relies on comparison of the electric current-related values of the examined injector with the model characteristics, which are representing the properly functioning injector. A model of the fuel injector in the form of the electric current waveform that describes the changes in the electric current and voltage during its work is presented in this article. Complex equations describing the fuel injector model under discussion account for the characteristics of the current variations, with no damage-induced modifications. Due to these, the modeled electric current/voltage waveform mirrors the real conditions. The use of a mathematical model describing the voltage–current phenomena occurring during the injector operation allows determining the actual beginning and duration of the injection. The model can also be used to develop new injector diagnostic methods that can be implemented in the engine controller (ECU).