bluuebunny/crossref_metadata_2025 huggingface dataset:
Quantum Walks in Hilbert Space of Lévy Matrices: Recurrences and Revivals
Authors: Iomin Alexander
Published: October 2021

The quantum evolution of wave functions controlled by the spectrum of Lévy random matrices is considered. An analytical treatment of quantum recurrences and revivals in the Hilbert space is performed in the framework of a theory of almost periodic functions. It is shown that the statistics of quantum recurrences in the Hilbert space of quantum systems is sensitive to the statistics of the corresponding quantum spectrum. In particular, it is shown that both the Poisson energy level statistics and the Brody distribution correspond to the power law of the quantum recurrences, while the Wigner–Dyson and Lévy–Smirnov statistics of the energy spectra are responsible for the exponential statistics of the quantum returns of the wave function.
Aberrant DNA Methylation Mediates the Transgenerational Risk of Metabolic and Chronic Disease Due to Maternal Obesity and Overnutrition
Authors: Li Yan , Pollock Carol A. , Saad Sonia
Published: October 2021

Maternal obesity is a rapidly evolving universal epidemic leading to acute and long-term medical and obstetric health issues, including increased maternal risks of gestational diabetes, hypertension and pre-eclampsia, and the future risks for offspring’s predisposition to metabolic diseases. Epigenetic modification, in particular DNA methylation, represents a mechanism whereby environmental effects impact on the phenotypic expression of human disease. Maternal obesity or overnutrition contributes to the alterations in DNA methylation during early life which, through fetal programming, can predispose the offspring to many metabolic and chronic diseases, such as non-alcoholic fatty liver disease, obesity, diabetes, and chronic kidney disease. This review aims to summarize findings from human and animal studies, which support the role of maternal obesity in fetal programing and the potential benefit of altering DNA methylation to limit maternal obesity related disease in the offspring.
Geoheritage of the Monchegorsk Igneous Layered Paleoproterozoic Intrusion (Kola Peninsula, Arctic Russia): Evaluation and Geotourism Opportunities
Authors: Huber Miłosz , Zhigunova Galina , Menshakova Maria , Iakovleva Olga , Karimova Maria
Published: October 2021

Monchegorsk is an intrusion complex of basic and ultrabasic rocks of the Paleoproterozoic age. This complex formed during active magmatic mobility that took place in NE Scandinavia 2.5 Ga years ago. These were the subject of intensive exploration and exploitation at the beginning of the 20th century, the latter carrying on through to the beginning of the 21st century. This contributed to the creation of some different forms of post-industrail mining infrastructure in the area. Many mining settlements, including Monchegorsk, mining plants, adits and quarries were established during this time, the relics of which are still present today. The Monchegorsk intrusions complex is formed by several fragmented massifs: Traviannaya, Kumuzhia, Nittis, Sopcha, Nyud, Poaz, and Monchetundra, the highest elevations of which reach up to 1000 m above sea level. These massifs form a landscape of “islands” and mountain ranges that have influence upon the regional landscape over several tens of kilometers. Their geography is characterized by numerous reliefs, glacial cirques, rocky thresholds with waterfalls and trough lakes. The potential of this region lies in the heritage of historical exploitation, numerous monuments of which have been preserved to this day. An important value is a landscape resulting from the relief of these mountains, highlighted by glacial activity in the Pleistocene. There are also interesting examples of Arctic fauna and flora, and of the rocks that form the bedrock in this intrusion. Some of the mineralization of these outcroppings can also be admired in the collections of the local museum that serves as a geocenter. The possibility of admiring relatively easily accessible views (the international route St. Petersburg–Murmansk–Kirkenes passes through the middle of the hills) and the interesting geology of the area abounding in rocks of mineralogical significance, their exposures, and history, along with the possibility of observing various post-industrail forms, make this area of great tourist potential. This article describes the most interesting exposures of outstanding tourist value and proposes routes connecting these points. It also discusses the problem of securing these exposures and the necessary tourist infrastructure, which is currently lacking.
Organic Remains in Early Christian Egyptian Metal Vessels: Investigation with Fourier Transform Infrared Spectroscopy and Gas Chromatography–Mass Spectrometry
Authors: Koupadi Kyriaki , Boyatzis Stamatis C. , Roumpou Maria , Kalogeropoulos Nick , Kotzamani Despoina
Published: October 2021

Organic remains preserved on eight copper alloy artifacts of the Byzantine Collection of the Benaki Museum with an Egyptian provenance were investigated, implementing a multi-analytical approach combining microscopy-FTIR and GC/MS. The transmission spectra of powder samples provided important information on the vessels regarding inorganic and organic components. In the latter case, subsequent extractions with a range of solvents allowed discrimination of components with different polarities and provided data on the suitability of the solvents for the acquisition of more informative spectra. GC/MS was implemented for the detailed characterization of the compounds present in the samples because of the complex nature of the residues preserved. A wide range of fatty acid oxidation products was identified, including a series of α, ω-dicarboxylic acids typical of such remains. In addition, vicinal dihydroxy-docosanoic and dihydroxy-eicosanoic acid, oxidation products of erucic and gondoic acid, respectively, were detected. Both are found in abundance in oils from plants belonging to the Brassicaceae family and imply their multiple uses in medieval Egypt.
Health Status and Barriers to Healthcare Access among “Son-in-Law Westerners”: A Qualitative Case Study in the Northeast of Thailand
Authors: Julchoo Sataporn , Pudpong Nareerut , Phaiyarom Mathudara , Sinam Pigunkaew , Khunakorncharatphong Anon , Suphanchaimat Rapeepong
Published: October 2021

The northeast of Thailand is well-known as a popular destination where many male Westerners marry Thai women and settle down there. However, little is known about their health and well-being. This study aims to explore the Western husbands’ health status and identify barriers hindering their healthcare access. A qualitative case study was conducted from November 2020 to May 2021. In-depth interviews and focus group discussions with 42 key informants who were involved with social and health issues among these expatriates were carried out. The social determinants framework was adapted for guiding the interviews. Data were triangulated with field notes, document reviews, and researchers’ observations. Inductive thematic analysis was applied. Results showed that most male expatriates who married Thai women in the northeast were in their retirement years and had non-communicable diseases, health risk behaviors, and mental health problems. Most of them did not purchase health insurance and held negative impressions toward Thai public hospitals’ quality of care, which was denoted as the main barrier to accessing healthcare services. Other significant barriers consisted of high treatment costs commonly charged by private hospitals and language issues. While the improvement of healthcare quality and the provision of friendly health services are important, public communication with foreign residents, especially male expatriates, is recommended to increase understanding and improve perceptions of the Thai healthcare systems. A regular population-based survey on the health and well-being of expatriates in Thailand, a cost study of a health insurance package, a survey study on willingness to pay for health insurance premiums, and a feasibility survey exploring the opportunity to establish either voluntary or compulsory health insurance among this group should be undertaken.
Local Inequalities in Health Behaviours: Longitudinal Findings from the Stockton-On-Tees Cohort Study
Authors: Akhter Nasima , Fairbairn Ross Stewart , Pearce Mark , Warren Jon , Kasim Adetayo , Bambra Clare
Published: October 2021

This paper provides a longitudinal examination of local inequalities in health behaviours during a period of austerity, exploring the role of ‘place’ in explaining these inequalities. Data from the Stockton-on-Tees prospective cohort study of 836 individuals were analysed and followed over 18 months (37% follow-up). Generalised estimating equation models estimated the deprivation gap in health behaviours (smoking status, alcohol use, fruit and vegetable consumption and physical activity practices) between the 20% most- and least-deprived neighborhoods (LSOAs), explored any temporal changes during austerity, and examined the underpinning role of compositional and contextual determinants. All health behaviours, except for frequent physical activity, varied significantly by deprivation (p ≤ 0.001). Smoking was lower in the least-deprived areas (OR 0.21, CI 0.14 to 0.30), while alcohol use (OR 2.75, CI 1.98 to 3.82) and fruit and vegetable consumption (OR 2.55, CI 1.80 to 3.62) were higher in the least-deprived areas. The inequalities were relatively stable throughout the study period. Material factors (such as employment, education and housing tenure) were the most-important and environmental factors the least-important explanatory factors. This study suggests that material factors are the most important ‘place’ determinants of health behaviours. Health promotion activities should better reflect these drivers.
Effect of Temporal Difference on Clinical Outcomes of Patients with Out-of-Hospital Cardiac Arrest: A Retrospective Study from an Urban City of Taiwan
Authors: Huang Han-Chun , Lee Tsung-Yu , Tsai Cheng-Han , Su Yao-Sing , Chen Yi-Rong , Yeh Ya-Ni , Hsu Chi-Feng , Tsai Ming-Jen
Published: October 2021

Circadian pattern influence on the incidence of out-of-hospital cardiac arrest (OHCA) has been demonstrated. However, the effect of temporal difference on the clinical outcomes of OHCA remains inconclusive. Therefore, we conducted a retrospective study in an urban city of Taiwan between January 2018 and December 2020 in order to investigate the relationship between temporal differences and the return of spontaneous circulation (ROSC), sustained (≥24 h) ROSC, and survival to discharge in patients with OHCA. Of the 842 patients with OHCA, 371 occurred in the daytime, 250 in the evening, and 221 at night. During nighttime, there was a decreased incidence of OHCA, but the outcomes of OHCA were significant poor compared to the incidents during the daytime and evening. After multivariate adjustment for influencing factors, OHCAs occurring at night were independently associated with lower probabilities of achieving sustained ROSC (aOR = 0.489, 95% CI: 0.285–0.840, p = 0.009) and survival to discharge (aOR = 0.147, 95% CI: 0.03–0.714, p = 0.017). Subgroup analyses revealed significant temporal differences in male patients, older adult patients, those with longer response times (≥5 min), and witnessed OHCA. The effects of temporal difference on the outcome of OHCA may be a result of physiological factors, underlying etiology of arrest, resuscitative efforts in prehospital and in-hospital stages, or a combination of factors.
Interdisciplinary Perspectives on Restraint Use in Aged Care
Authors: Breen Juanita , Wimmer Barbara C. , Smit Chloé C.H. , Courtney-Pratt Helen , Lawler Katherine , Salmon Katharine , Price Andrea , Goldberg Lynette R.
Published: October 2021

Restraint use in Australian residential aged care has been highlighted by the media, and investigated by researchers, government and advocacy bodies. In 2018, the Royal Commission into Aged Care selected ‘Restraint’ as a key focus of inquiry. Subsequently, Federal legislation was passed to ensure restraint is only used in residential aged care services as the ‘last resort’. To inform and develop Government educational resources, we conducted qualitative research to gain greater understanding of the experiences and attitudes of aged care stakeholders around restraint practice. Semi-structured interviews were held with 28 participants, comprising nurses, care staff, physicians, physiotherapists, pharmacists and relatives. Two focus groups were also conducted to ascertain the views of residential and community aged care senior management staff. Data were thematically analyzed using a pragmatic approach of inductive and deductive coding and theme development. Five themes were identified during the study: 1. Understanding of restraint; 2. Support for legislation; 3. Restraint-free environments are not possible; 4. Low-level restraint; 5. Restraint in the community is uncharted. Although most staff, health practitioners and relatives have a basic understanding of restraint, more education is needed at a conceptual level to enable them to identify and avoid restraint practice, particularly ‘low-level’ forms and chemical restraint. There was strong support for the new restraint regulations, but most interviewees admitted they were unsure what the legislation entailed. With regards to resources, stakeholders wanted recognition that there were times when restraint was necessary and advice on what to do in these situations, as opposed to unrealistic aspirations for restraint-free care. Stakeholders reported greater oversight of restraint in residential aged care but specified that community restraint use was largely unknown. Research is needed to investigate the extent and types of restraint practice in community aged care.
The Capacity to Be Alone Moderates Psychopathological Symptoms and Social Networks Use in Adolescents during the COVID-19 Pandemic
Authors: Cimino Silvia , Cerniglia Luca
Published: October 2021

During the COVID-19 pandemic, adolescents could not leave their house freely, meet up with friends, or attend school; previous literature showed that youths under enforced confinement or quarantine were five times more likely to suffer from psychopathological symptoms and use social networks sites (SNs) greatly. This study aimed to verify whether the quality of the parent-adolescent relationship could predict youths’ psychopathological symptoms and their SN use during the pandemic, and to evaluate the possible moderator role of their the capacity to be alone. Seven hundred and thirty-nine (n = 739) adolescents were recruited from the general population during the COVID-19 lockdown in Italy, and they were administered The Capacity to be Alone Scale, The BSMAS, the YSR, and the Perceived Filial Self-efficacy Scale. Our results confirmed a direct effect of the perceived filial self-efficacy on the psychopathological symptoms so that a poorer perceived quality of the relationship with the caregivers predicted higher psychopathological symptoms in youths. Moreover, greater social networks use was predictive of psychopathological symptoms in adolescents. Our results also showed a significant interaction effect between adolescents’ perceived filial efficacy and the capacity to be alone on SN use and on psychopathological symptoms. These results suggest that youths’ response to the confinement during the pandemic is influenced both by individual characteristics (the capacity to be alone) and by relational variables (the perceived filial self-efficacy).
A Short Version of the Physical Activity Enjoyment Scale: Development and Psychometric Properties
Authors: Chen Cheng , Weyland Susanne , Fritsch Julian , Woll Alexander , Niessner Claudia , Burchartz Alexander , Schmidt Steffen C. E. , Jekauc Darko
Published: October 2021

Objective: The purposes of this paper were to (a) develop a new short, theory-driven, version of the physical activity enjoyment scale (PACES-S) using content analysis; and (b) subsequently to measure the psychometric properties (construct validity, internal consistency, test–retest reliability, and concurrent validity) of the PACES-S for adolescents. Methods: Six experts used a four-point Likert scale to assess the content validity of each of the 16 items of the physical activity enjoyment scale according to a provided definition of physical activity enjoyment. Based on the results, exploratory factor analysis was used to analyze survey data from a longitudinal study of 182 individuals (Measure 1 of Study 1: 15.75 ± 3.39 yrs; 56.6% boys, 43.4% girls), and confirmatory factor analysis (Measure 2 of Study 1: 15.69 ± 3.44 yrs; 56.3% boys, 43.7% girls) was used to analyze the survey data from a cross-sectional study of 3219 individuals (Study 2; 15.99 ± 3.10 yrs; 47.8% boys, 52.2% girls) to assess the construct validity of the new measure. To assess the reliability, test–retest reliability was assessed in Study 1 and internal consistency in Study 1 and 2. For the concurrent validity, correlations with self-reported and device-based physical activity behavior were assessed in both studies. Results: Four out of sixteen items were selected for PACES-S. Exploratory factor analysis and confirmatory factor analyses identified and supported its factorial validity (χ2 = 53.62, df = 2, p < 0.001; RMSEA = 0.073; CFI = 0.99; RFI = 0.96; NFI = 0.99; TLI = 0.96; IFI = 0.99). Results showed good test–retest reliability (r = 0.76) and internal consistency (a = 0.82 to 0.88). Regarding concurrent validity, the results showed positive correlations with a physical activity questionnaire (Study 1: r = 0.36), with a physical activity diary (Study 1: r = 0.44), and with accelerometer-recorded data (Study 1: r = 0.32; Study 2: r = 0.21). Conclusions: The results indicate that PACES-S is a reliable and valid instrument that may be particularly useful to measure physical activity enjoyment in large-scale studies. It shows comparable measurement properties as the long version of PACES.
Impact of the COVID-19 Lockdown in a European Regional Monitoring Network (Spain): Are We Free from Pollution Episodes?
Authors: Gangoiti Gotzon , de Blas Maite , Gómez Maria Carmen , Rodríguez-García Ana , Torre-Pascual Eduardo , García-Ruiz Estíbaliz , Sáez de Cámara Estibaliz , Zuazo Iñaki , García José Antonio , Valdenebro Verónica
Published: October 2021

The impact of the lockdown, during the period from March to June in 2020, upon the air quality of the Basque Country in northern Spain is analyzed. The evaluation accounts for the meteorology of the period. Daily and sub-daily analysis of aerosol and ozone records show that the territory was repeatedly affected by episodes of pollutants from outer regions. Three episodes of PM10 and ten of PM2.5 were caused by transported anthropogenic European sulfates, African dust, and wildland fires. The region, with a varied orographic climatology, shows high and diverse industrial activity. Urban and interurban road traffic of the region decreased by 49% and 53%, respectively, whereas industrial activity showed a lower reduction of 20%. Consequently, the average concentrations of NO2 in the cities during the period fell to 12.4 µg·m−3 (−45%). Ozone showed up to five exceedances of the WHOAQG for the daily maximum 8-h average in both rural and urban sites, associated with transport through France and the Bay of Biscay, under periods of European blocking anticyclones. However, averages showed a moderate decrease (−11%) in rural environments, in line with the precursor reductions, and disparate changes in the cities, which reproduced the weekend effect of their historical records. The PM10 decreased less than expected (−10% and −21%, in the urban and rural environments, respectively), probably caused by the modest decrease of industrial activity around urban sites and favorable meteorology for secondary aerosol formation, which could also influence the lower changes observed in the PM2.5 (−1% and +3% at the urban and rural sites, respectively). Consequently, in a future low NOx traffic emission scenario, the inter-regional PM and ozone control will require actions across various sectors, including the industry and common pollution control strategies.
Novel and Annotated Long Noncoding RNAs Associated with Ischemia in the Human Heart
Authors: Ward Zoe , Schmeier Sebastian , Saddic Louis , Sigurdsson Martin I. , Cameron Vicky A. , Pearson John , Miller Allison , Morley-Bunker Arthur , Gorham Josh , Seidman Jonathan G. , Moravec Christine S. , Sweet Wendy E. , Aranki Sary F. , Body Simon , Muehlschlegel Jochen D. , Pilbrow Anna P.
Published: October 2021

Background: Long noncoding RNAs (lncRNAs) have been implicated in the pathogenesis of cardiovascular diseases. We aimed to identify novel lncRNAs associated with the early response to ischemia in the heart. Methods and Results: RNA sequencing data gathered from 81 paired left ventricle samples from patients undergoing cardiopulmonary bypass was collected before and after a period of ischemia. Novel lncRNAs were validated with Oxford Nanopore Technologies long-read sequencing. Gene modules associated with an early ischemic response were identified and the subcellular location of selected lncRNAs was determined with RNAscope. A total of 2446 mRNAs, 270 annotated lncRNAs and one novel lncRNA differed in response to ischemia (adjusted p < 0.001, absolute fold change >1.2). The novel lncRNA belonged to a gene module of highly correlated genes that also included 39 annotated lncRNAs. This module associated with ischemia (Pearson correlation coefficient = −0.69, p = 1 × 10−23) and activation of cell death pathways (p < 6 × 10−9). A further nine novel cardiac lncRNAs were identified, of which, one overlapped five cis-eQTL eSNPs for the gene RWD Domain-Containing Sumoylation Enhancer (RWDD3) and was itself correlated with RWDD3 expression (Pearson correlation coefficient −0.2, p = 0.002). Conclusion: We have identified 10 novel lncRNAs, one of which was associated with myocardial ischemia and may have potential as a novel therapeutic target or early marker for myocardial dysfunction.
Cytokines as Biomarkers in Systemic Lupus Erythematosus: Value for Diagnosis and Drug Therapy
Authors: Idborg Helena , Oke Vilija
Published: October 2021

Systemic Lupus Erythematosus (SLE) is a chronic autoimmune disease. The disease is characterized by activation and dysregulation of both the innate and the adaptive immune systems. The autoimmune response targets self-molecules including cell nuclei, double stranded DNA and other intra and extracellular structures. Multiple susceptibility genes within the immune system have been identified, as well as disturbances in different immune pathways. SLE may affect different organs and organ systems, and organ involvement is diverse among individuals. A universal understanding of pathophysiological mechanism of the disease, as well as directed therapies, are still missing. Cytokines are immunomodulating molecules produced by cells of the immune system. Interferons (IFNs) are a broad group of cytokines, primarily produced by the innate immune system. The IFN system has been observed to be dysregulated in SLE, and therefore IFNs have been extensively studied with a hope to understand the disease mechanisms and identify novel targeted therapies. In several autoimmune diseases identification and subsequent blockade of specific cytokines has led to successful therapies, for example tumor necrosis factor-alpha (TNF-α) inhibition in rheumatoid arthritis. Authors of this review have sought corresponding developments in SLE. In the current review, we cover the actual knowledge on IFNs and other studied cytokines as biomarkers and treatment targets in SLE.
Two TGA Transcription Factor Members from Hyper-Susceptible Soybean Exhibiting Significant Basal Resistance to Soybean mosaic virus
Authors: Jiang Hua , Gu Shengyu , Li Kai , Gai Junyi
Published: October 2021

TGA transcription factors (TFs) exhibit basal resistance in Arabidopsis, but susceptibility to a pathogen attack in tomatoes; however, their roles in soybean (Glycine max) to Soybean mosaic virus (SMV) are unknown. In this study, 27 TGA genes were isolated from a SMV hyper-susceptible soybean NN1138-2, designated GmTGA1~GmTGA27, which were clustered into seven phylogenetic groups. The expression profiles of GmTGAs showed that the highly expressed genes were mainly in Groups I, II, and VII under non-induction conditions, while out of the 27 GmTGAs, 19 responded to SMV-induction. Interestingly, in further transient N. benthamiana-SMV pathosystem assay, all the 19 GmTGAs overexpressed did not promote SMV infection in inoculated leaves, but they exhibited basal resistance except one without function. Among the 18 functional ones, GmTGA8 and GmTGA19, with similar motif distribution, nuclear localization sequence and interaction proteins, showed a rapid response to SMV infection and performed better than the others in inhibiting SMV multiplication. This finding suggested that GmTGA TFs may support basal resistance to SMV even from a hyper-susceptible source. What the mechanism of the genes (GmTGA8, GmTGA19, etc.) with basal resistance to SMV is and what their potential for the future improvement of resistance to SMV in soybeans is, are to be explored.
Mechanical Properties of Human Concentrated Growth Factor (CGF) Membrane and the CGF Graft with Bone Morphogenetic Protein-2 (BMP-2) onto Periosteum of the Skull of Nude Mice
Authors: Kabir Md. Arafat , Hirakawa Akihiro , Zhu Bowen , Yokozeki Kenji , Shakya Mamata , Huang Bingzhen , Akazawa Toshiyuki , Todoh Masahiro , Murata Masaru
Published: October 2021

Concentrated growth factor (CGF) is 100% blood-derived, cross-linked fibrin glue with platelets and growth factors. Human CGF clot is transformed into membrane by a compression device, which has been widely used clinically. However, the mechanical properties of the CGF membranes have not been well characterized. The aims of this study were to measure the tensile strength of human CGF membrane and observe its behavior as a scaffold of BMP-2 in ectopic site over the skull. The tensile test of the full length was performed at the speed of 2mm/min. The CGF membrane (5 × 5 × 2 mm3) or the CGF/BMP-2 (1.0 μg) membrane was grafted onto the skull periosteum of nude mice (5-week-old, male), and harvested at 14 days after the graft. The appearance and size of the CGF membranes were almost same for 7 days by soaking at 4 °C in saline. The average values of the tensile strength at 0 day and 7 days were 0.24 MPa and 0.26 MPa, respectively. No significant differences of both the tensile strength and the elastic modulus were found among 0, 1, 3, and 7 days. Supra-periosteal bone induction was found at 14 days in the CGF/BMP-2, while the CGF alone did not induce bone. These results demonstrated that human CGF membrane could become a short-term, sticky fibrin scaffold for BMP-2, and might be preserved as auto-membranes for wound protection after the surgery.
Platelet-Rich Fibrin Decreases the Inflammatory Response of Mesenchymal Cells
Authors: Kargarpour Zahra , Nasirzade Jila , Panahipour Layla , Miron Richard J. , Gruber Reinhard
Published: October 2021

Chronic inflammation is a pathological process where cells of the mesenchymal lineage become a major source of inflammatory mediators. Platelet-rich fibrin (PRF) has been shown to possess potent anti-inflammatory activity in macrophages, but its impact on mesenchymal cells has not been investigated. The aim of this study was, therefore, to expose mesenchymal cells to inflammatory cytokines together with lysates generated from liquid platelet-poor plasma (PPP), the cell-rich buffy coat layer (BC; concentrated-PRF or C-PRF), and the remaining red clot layer (RC), following centrifugation of blood. Heating PPP generates an albumin gel (Alb-gel) that when mixed back with C-PRF produces Alb-PRF. Membranes prepared from solid PRF were also subjected to lysis. We report here that lysates of PPP, BC, and PRF decreased the cytokine-induced expression of interleukin 6 (IL6) and nitric oxide synthase (iNOS) in the bone marrow-derived ST2 cells. Consistently, PPP, BC, and PRF greatly decreased the phosphorylation and nuclear translocation of p65 in ST2 cells. The inflammatory response caused by Pam3CSK4 was reduced accordingly. Moreover, PPP, BC, and PRF reduced the enhanced expression of inflammatory mediators IL6 and iNOS in 3T3-L1 pre-adipocyte mesenchymal cells, and iNOS and CCL5 in murine calvarial cells. Surprisingly, PRF lysates were not effective in reducing the inflammatory response of human gingival fibroblasts and HSC2 epithelial cells. The data from the present study suggest that both liquid PRF and solid PRF exert potent anti-inflammatory activity in murine mesenchymal cells.
Prevalence and Characteristics of Phenicol-Oxazolidinone Resistance Genes in Enterococcus Faecalis and Enterococcus Faecium Isolated from Food-Producing Animals and Meat in Korea
Authors: Kim Eiseul , Shin So-Won , Kwak Hyo-Sun , Cha Min-Hyeok , Yang Seung-Min , Gwak Yoon-Soo , Woo Gun-Jo , Kim Hae-Yeong
Published: October 2021

The use of phenicol antibiotics in animals has increased. In recent years, it has been reported that the transferable gene mediates phenicol-oxazolidinone resistance. This study analyzed the prevalence and characteristics of phenicol-oxazolidinone resistance genes in Enterococcus faecalis and Enterococcus faecium isolated from food-producing animals and meat in Korea in 2018. Furthermore, for the first time, we reported the genome sequence of E. faecalis strain, which possesses the phenicol-oxazolidinone resistance gene on both the chromosome and plasmid. Among the 327 isolates, optrA, poxtA, and fexA genes were found in 15 (4.6%), 8 (2.5%), and 17 isolates (5.2%), respectively. Twenty E. faecalis strains carrying resistance genes belonged to eight sequence types (STs), and transferability was found in 17 isolates. The genome sequences revealed that resistant genes were present in the chromosome or plasmid, or both. In strains EFS17 and EFS108, optrA was located downstream of the ermA and ant(9)-1 genes. The strains EFS36 and EFS108 harboring poxtA-encoding plasmid cocarried fexA and cfr(D). These islands also contained IS1216E or the transposon Tn554, enabling the horizontal transfer of the phenicol-oxazolidinone resistance with other antimicrobial-resistant genes. Our results suggest that it is necessary to promote the prudent use of antibiotics through continuous monitoring and reevaluation.
Mitochondria as a Cellular Hub in Infection and Inflammation
Authors: Andrieux Pauline , Chevillard Christophe , Cunha-Neto Edecio , Nunes João Paulo Silva
Published: October 2021

Mitochondria are the energy center of the cell. They are found in the cell cytoplasm as dynamic networks where they adapt energy production based on the cell’s needs. They are also at the center of the proinflammatory response and have essential roles in the response against pathogenic infections. Mitochondria are a major site for production of Reactive Oxygen Species (ROS; or free radicals), which are essential to fight infection. However, excessive and uncontrolled production can become deleterious to the cell, leading to mitochondrial and tissue damage. Pathogens exploit the role of mitochondria during infection by affecting the oxidative phosphorylation mechanism (OXPHOS), mitochondrial network and disrupting the communication between the nucleus and the mitochondria. The role of mitochondria in these biological processes makes these organelle good targets for the development of therapeutic strategies. In this review, we presented a summary of the endosymbiotic origin of mitochondria and their involvement in the pathogen response, as well as the potential promising mitochondrial targets for the fight against infectious diseases and chronic inflammatory diseases.
α-Galactosidase a Deficiency in Fabry Disease Leads to Extensive Dysregulated Cellular Signaling Pathways in Human Podocytes
Authors: Jehn Ulrich , Bayraktar Samet , Pollmann Solvey , Van Marck Veerle , Weide Thomas , Pavenstädt Hermann , Brand Eva , Lenders Malte
Published: October 2021

Fabry disease (FD) is caused by mutations in the α-galactosidase A (GLA) gene encoding the lysosomal AGAL enzyme. Loss of enzymatic AGAL activity and cellular accumulation of sphingolipids (mainly globotriaosylcermide) may lead to podocyturia and renal loss of function with increased cardiovascular morbidity and mortality in affected patients. To identify dysregulated cellular pathways in FD, we established a stable AGAL-deficient podocyte cell line to perform a comprehensive proteome analysis. Imbalanced protein expression and function were analyzed in additional FD cell lines including endothelial, epithelial kidney, patient-derived urinary cells and kidney biopsies. AGAL-deficient podocytes showed dysregulated proteins involved in thermogenesis, lysosomal trafficking and function, metabolic activity, cell-cell interactions and cell cycle. Proteins associated with neurological diseases were upregulated in AGAL-deficient podocytes. Rescues with inducible AGAL expression only partially normalized protein expression. A disturbed protein expression was confirmed in endothelial, epithelial and patient-specific cells, pointing toward fundamental pathway disturbances rather than to cell type-specific alterations in FD. We conclude that a loss of AGAL function results in profound changes of cellular pathways, which are ubiquitously in different cell types. Due to these profound alterations, current approved FD-specific therapies may not be sufficient to completely reverse all dysregulated pathways.
Platelets Contribution to Thrombin Generation in Philadelphia-Negative Myeloproliferative Neoplasms: The “Circulating Wound” Model
Authors: Lucchesi Alessandro , Napolitano Roberta , Bochicchio Maria Teresa , Giordano Giulio , Napolitano Mariasanta
Published: October 2021

Current cytoreductive and antithrombotic strategies in MPNs are mostly based on cell counts and on patient’s demographic and clinical history. Despite the numerous studies conducted on platelet function and on the role of plasma factors, an accurate and reliable method to dynamically quantify the hypercoagulability states of these conditions is not yet part of clinical practice. Starting from our experience, and after having sifted through the literature, we propose an in-depth narrative report on the contribution of the clonal platelets of MPNs—rich in tissue factor (TF)—in promoting a perpetual procoagulant mechanism. The whole process results in an unbalanced generation of thrombin and is self-maintained by Protease Activated Receptors (PARs). We chose to define this model as a “circulating wound”, as it indisputably links the coagulation, inflammation, and fibrotic progression of the disease, in analogy with what happens in some solid tumours. The platelet contribution to thrombin generation results in triggering a vicious circle supported by the PARs/TGF-beta axis. PAR antagonists could therefore be a good option for target therapy, both to contain the risk of vascular events and to slow the progression of the disease towards end-stage forms. Both the new and old strategies, however, will require tools capable of measuring procoagulant or prohaemorrhagic states in a more extensive and dynamic way to favour a less empirical management of MPNs and their potential clinical complications.
Comprehensive Identification of Deleterious TP53 Missense VUS Variants Based on Their Impact on TP53 Structural Stability
Authors: Tam Benjamin , Sinha Siddharth , Qin Zixin , Wang San Ming
Published: October 2021

TP53 plays critical roles in maintaining genome stability. Deleterious genetic variants damage the function of TP53, causing genome instability and increased cancer risk. Of the large quantity of genetic variants identified in TP53, however, many remain functionally unclassified as variants of unknown significance (VUS) due to the lack of evidence. This is reflected by the presence of 749 (42%) VUS of the 1785 germline variants collected in the ClinVar database. In this study, we addressed the deleteriousness of TP53 missense VUS. Utilizing the protein structure-based Ramachandran Plot-Molecular Dynamics Simulation (RPMDS) method that we developed, we measured the effects of missense VUS on TP53 structural stability. Of the 340 missense VUS tested, we observed deleterious evidence for 193 VUS, as reflected by the TP53 structural changes caused by the VUS-substituted residues. We compared the results from RPMDS with those from other in silico methods and observed higher specificity of RPMDS in classification of TP53 missense VUS than these methods. Data from our current study address a long-standing challenge in classifying the missense VUS in TP53, one of the most important tumor suppressor genes.
A Domain-Adaptable Heterogeneous Information Integration Platform: Tourism and Biomedicine Domains
Authors: Gil Rafael Muñoz , de Buenaga Rodríguez Manuel , Galisteo Fernando Aparicio , Páez Diego Gachet , García-Cuesta Esteban
Published: October 2021

In recent years, information integration systems have become very popular in mashup-type applications. Information sources are normally presented in an individual and unrelated fashion, and the development of new technologies to reduce the negative effects of information dispersion is needed. A major challenge is the integration and implementation of processing pipelines using different technologies promoting the emergence of advanced architectures capable of processing such a number of diverse sources. This paper describes a semantic domain-adaptable platform to integrate those sources and provide high-level functionalities, such as recommendations, shallow and deep natural language processing, text enrichment, and ontology standardization. Our proposed intelligent domain-adaptable platform (IDAP) has been implemented and tested in the tourism and biomedicine domains to demonstrate the adaptability, flexibility, modularity, and utility of the platform. Questionnaires, performance metrics, and A/B control groups’ evaluations have shown improvements when using IDAP in learning environments.
Football Misinformation Matrix: A Comparative Study of 2020 Winter Transfer News in Four European Sports Media Outlets
Authors: Rojas Torrijos José Luis , Mello Matheus Simoes
Published: October 2021

Mainstream sports media generate a football information overload that sometimes makes it difficult to separate rumours from real news. Accordingly, this paper analyses the level of misinformation in the coverage of the 2020 winter football transfer window in four leading European digital sports media outlets: Marca (Spain), A Bola (Portugal), La Gazzetta (Italy) and The Guardian Sport (Britain). The methodology used was based on the content analysis of hundreds of news pieces and tweets posted on these outlets’ football homepages and Twitter handles over a month. To examine to what extent this coverage may have been speculative, misleading or false, the misinformation matrix developed by the fact-checking organisation First Draft News was employed to classify five different types of inaccuracies in sports reporting. A system was also created to determine how many reported rumours finally turned out to be true, which sources were more reliable and what outlets resulted more accurate. The findings reveal that the four digital media published a larger amount of non-factual content about likely football deals rather than sealed transfers. Speculative reporting prevailed in the coverage of the top teams in each league, on which the media outlets placed the accent, whereas reporting about minor clubs was based more on factual news.
Evolutionary Game Analysis of Blockchain Technology Preventing Supply Chain Financial Risks
Authors: Sun Rui , He Dayi , Su Huilin
Published: October 2021

Because of the risks existing in supply chain finance, taking accounts receivable factoring business as the research object, this paper uses the evolutionary game method to analyzes the factors affecting the decision-making of the participants in supply chain finance, constructs an evolutionary game model between small and medium-sized enterprises and financial institutions, and analyzes the mechanism of blockchain to solve the financial risks of the supply chain by comparing the changes of evolutionary stability strategies before and after the introduction of blockchain technology. This paper aims to reduce financing risks by analyzing the mechanism of blockchain technology in supply chain finance. It is found that, firstly, blockchain technology can reduce the credit risk of financial institutions and solve financing problem. Credit risk plays a decisive role in whether financial institutions accept financing business decisions. Blockchain technology can reduce the operational risk of financial institutions and improve the business income of financial institutions. Secondly, the strict regulatory environment formed by blockchain technology makes the default behavior of small and medium-sized enterprises and core enterprises in a high-risk state at all times. No matter the profit distribution proportion that small and medium-sized enterprises can obtain through collusion, they will not choose to default, which effectively solves the paradox that small and medium-sized enterprises cannot obtain loans from financial institutions despite the increased probability of compliance. Then, the evolutionary game between financial institutions and small and medium-sized enterprises is balanced in that financial institutions accept business applications, small and medium-sized enterprises abide by the contract, and the convergence effect is better. Therefore, blockchain technology not only reduces the financing risk of financial institutions but also helps to solve the financing problems of small and medium-sized enterprises.
Changes in Frequency and Mode Shapes Due to Damage in Steel–Concrete Composite Beam
Authors: Jarosińska Małgorzata , Berczyński Stefan
Published: October 2021

This study presents an analysis of changes in the vibration frequency and mode of vibration of a composite beam due to damage. A steel–concrete composite beam was considered, for which numerical analysis (RFE model) and experimental tests were conducted. Two levels of damage were introduced to the beam. To determine the changes in the mode of vibration before and after the damage, the modal assurance criterion (MAC) and its partial variation (PMAC) were applied. The curvature damage factor (CDF) was used to determine the changes in the modal curvature. The natural frequencies were sensitive to the introduced damage. The results show that the MAC is not effective in determining the location of damage in the connection plane. Two different coefficients were introduced to locate the damage. The PMAC was used for sections of subsequent modes of vibration and allowed effectively locating the damage. The CDF considered simultaneous changes in the curvatures of all vibration modes and was effective in locating the damage in the connection plane.
Frictional Properties of the TiNbTaZrO Orthodontic Wire—A Laboratory Comparison to Popular Archwires
Authors: Schmeidl Krzysztof , Wieczorowski Michal , Grocholewicz Katarzyna , Mendak Michal , Janiszewska-Olszowska Joanna
Published: October 2021

Background. This study aimed to determine the kinetic frictional force (FF) of the recently produced TiNbTaZrO (Gummetal) orthodontic wire and compare it to the widely used wires of stainless steel (SS), nickel-titanium (NiTi), cobalt-chromium (CoCr) and titanium-molybdenum (TiMo) alloys. Methods. Five types of 0.016″ × 0.022″ wires were ligated with elastic ligatures to 0.018″ × 0.025″ SS brackets. The dynamic FFs between the brackets and ligated wires were measured utilizing a specialized tensile tester machine. Prior sample sizes for different archwires were conducted using power analysis for the general linear models. The existence of significant differences in FF between examined materials was initially confirmed by the one-way analysis of variance (ANOVA) with further evidence of pairwise differences by Tukey’s Honest Significant Difference test. Results. The pairwise differences between means of kinetic FFs for NiTi, CoCr, and Gummetal wires were not statistically significant (adjusted p-value > 0.05). Stainless steel alloy presented the lowest FF values significantly different from other groups (adjusted p-value < 0.05). On the contrary, TiMo wires showed significantly greater FFs (adjusted p-value < 0.05) than other alloys. Conclusions. Gummetal orthodontic wire exhibits similar frictional resistance as NiTi and CoCr wires. Bendable TiNbTaZrO wire might be used for sliding mechanics due to its favorable frictional properties.
Experimental Study on the Mechanical Properties and Durability of High-Content Hybrid Fiber–Polymer Concrete
Authors: Zhao Chaohua , Yi Zhijian , Wu Weiwei , Zhu Zhiwei , Peng Yi , Liu Jie
Published: October 2021

Polymer-modified concrete and fiber concrete are two excellent paving materials that improve the performance of some concrete, but the performance of single application material is still limited. In this paper, polymer-modified concrete with strong deformation and fiber concrete with obvious crack resistance and reinforcement effect were compounded by using the idea of composite material design so as to obtain a high-performance pavement material. The basic mechanical properties of high-content hybrid fiber–polymer-modified concrete, such as workability, compression, flexural resistance, and environmental durability (such as sulfate resistance) were studied by using the test regulations of cement concrete in China. The main results were as follows. (1) The hybrid fiber–polymer concrete displayed reliable working performance, high stiffness, and a modulus of elasticity as high as 35.93 GPa. (2) The hybrid fiber–polymer concrete had a compressive strength of 52.82 MPa, which was 31.2% higher than that of the plain C40 concrete (40.25 MPa); the strength of bending of the hybrid concrete was 11.51 MPa, 191.4% higher than that of the plain concrete (3.95 MPa). (3) The corrosion resistance value of the hybrid fiber–polymer concrete was 81.31%, indicating its adjustability to sulfate attack environments. (4) According to cross-sectional scanning electron microscope (SEM) images, the hybrid fiber–polymer concrete was seemingly more integrated with a dense layer of cementing substance on its surface along with fewer microholes and microcracks as when compared to the ordinary concrete. The research showed that hybrid fiber–polymer concrete had superior strength and environmental erosion resistance and was a pavement material with superior mechanical properties.