bluuebunny/crossref_metadata_2025 huggingface dataset:
Impact of Principals' Leadership Behaviors on Teachers' Job Satisfaction and Performance: Multiple-Case Study of Kindergartens in China Through the Lens of Social Exchange Theory
Authors:   Korean Association For Learner-Centered Curriculum And Instruction, Qi Xin , Yoon Man-Keun
Published: October 2024

Objectives This study aims to identify the leadership behaviors of kindergarten principals that influence teachers' job satisfaction and performance and to explore the impact of leadership on teachers through the lens of the Social Exchange Theory.
Methods This study employs a multiple-case qualitative approach, focusing on principals and teachers from Q, S, and L kindergartens in Beijing. Data was collected through observations, interviews, and artifacts collection. The Constant Comparison Method, developed by Glaser and Strauss, was used to analyze the data. A total of 256 meanings, 49 concepts, 18 sub-categories, and 8 superordinate categories were established.
Results The findings show that principals' behaviors such as “unselfishly looking after teachers,” “managing the organization warmly and effectively,” and “interacting with teachers like friends” foster a familial atmosphere and help teachers find purpose in their roles. These behaviors motivated teachers to lead a desire for reciprocal relationships while expressing a strong commitment to their work.
Conclusions Grounded in Social Exchange Theory, the study elucidates the impact of principals' leadership on teachers' job satisfaction and performance, offering a theoretical foundation for designing leadership training programs for principals. Additionally, the identified leadership behaviors provide practical guidelines for principals' self-assessment and improvement, enabling them to better focus on nurturing a high-quality teaching team.
Literature Circles Program for Middle School Students Operational Case Study: Focusing on the influence on literary interpretation
Authors:   Korean Association For Learner-Centered Curriculum And Instruction, Yi JeEun , Choi InJa
Published: October 2024

Objectives The purpose of this study is to determine the impact on literary interpretation after implementing a literature circles program for middle school students.
Methods For this purpose, eight students (five males and three females) in the first and second grades of a middle school in Gwangjin-gu, Seoul, Korea, participated in 10 sessions of literature circles activities. The participants' responses at each stage of the literature circles program were analysed through a combination of transcripts, activity products, and observation journals.
Results Through the literature circles program, participants deepened their understanding of the works through active interaction with community members. Participants actively shared their responses to the literature, and in the process of understanding the literature, collaborative discourse was used to construct shared meaning, and interpretation was enriched through interaction. Sharing personal interpretive responses led to emotional expression and generalisation of empathy through receptive attitudes, while active interaction through interpretive questions led to deeper interpretation.
Conclusions Literature circles program have shown that active interaction, which increases voluntary participation and deepens understanding of literary works, is conducive to improving literary interpretation skills. They also show that interaction enables an active and engaged literary experience through collaborative meaning making and enriched understanding of texts. Literary interpretation through literature circles proposes a new approach to traditional literary education and social reading of literature, which is expected to contribute to strengthening literary education and the social role of literature by enabling a more active and in-depth understanding of literary works. It is also hoped that literature circles will be activated through active discourse in both in-school and out-of-school curricula.
The Mediating Effect of Relationship Authenticity on the Relationship Between the Disconnection and Rejection Schema and Marital Intimacy: Actor and Partner Effects
Authors:   Korean Association For Learner-Centered Curriculum And Instruction, Yoo Ji-Bin , Hong Jung-Soon
Published: October 2024

Objectives The purpose of this study was to examine the mediation effect of relationship authenticity on the relationship between disconnection and rejection scheme and marital intimacy in couples, using the Actor-Partner Interdependence Model.
Methods Using a sample of 270 couples (540 individuals) residing in Gyeonggi Province, this study measured disconnection and rejection schemas, relationship authenticity, and marital intimacy to examine the Actor Effect and Partner Effect based on the Actor-Partner Interdependence Model (APIM). The relative significance of the effect sizes between paths was confirmed through equivalence constraints. SPSS and AMOS programs were used, and the significance of individual indirect effects was tested using the bootstrapping method.
Results First, significant differences were found in relationship authenticity and marital intimacy as the results of examining the differences in variables by gender. Second, analysis of the impact of the disconnection and rejection schema on relationship authenticity showed that both husbands and wives exhibited the Actor Effect, while the Partner Effect was found to be statistically significant only for wives. Third, in assessing the impact of relationship authenticity on marital intimacy, both husbands and wives demonstrated the Actor Effect, with the Partner Effect being statistically significant only for wives. Finally, a complete mediation effect was found in examining the mediating effect of relationship authenticity on the relationship between the disconnection and rejection schema and marital intimacy. Both husbands and wives exhibited the Actor Effect, where perceived marital intimacy decreased due to reduced relational authenticity caused by disconnection and rejection schemas. Additionally, the Partner Effect was observed, which reduces marital intimacy for wives when the disconnection and rejection schema of husbands lowers the wives' relationship authenticity.
Conclusions It was confirmed that examining disconnection and rejection schemas and improving relationship authenticity are important for enhancing marital intimacy. Based on these findings, implications for the study and suggestions for further research are discussed.
Enhancing Food Image Classification with Particle Swarm Optimization on NutriFoodNet and Data Augmentation Parameters
Authors: Sreetha E S  , G Naveen Sundar  , D Narmadha
Published: October 2024

A convolutional neural network (CNN) architecture, NutriFoodNet, enhanced through Particle Swarm Optimization (PSO) is suggested in this paper to optimize data augmentation parameters and key hyperparameters, specifically designed for food image recognition. Accurate food image classification plays a vital function in various applications, including nutrition management, dietary assessment, and healthcare, as it aids in the automated recognition and analysis of food items from images. The implementation aimed to improve classification accuracy on the Food101 dataset. Initially, the NutriFoodNet model achieved an accuracy of 97.3%. By applying PSO, the model's performance was further refined, resulting in an increased accuracy of 98.5%. This optimized system was benchmarked against state-of-the-art architectures, including ResNet-18, ResNet-50, and Inception V3, showcasing its exceptional performance. The proposed system highlights the efficiency of PSO in fine-tuning augmentation parameters and CNN hyperparameters, leading to significant improvements in model accuracy for food image classification tasks. This advancement underscores the potential of enhanced food image classification systems in contributing to better dietary monitoring and healthcare outcomes.
Em busca das fontes: a versão abreviada da tradução espanhola da obra de Isaac de Nínive no impresso sevilhano de 1497
Authors: Cambraia César Nardelli
Published: October 2024

Este estudo teve como objetivo discutir a questão das fontes da versão abreviada da tradução espanhola obra de Isaac de Nínive presente no impresso sevilhano de 1497. Foram consideradas duas hipóteses: a da contiguidade material (a fonte para a versão abreviada estaria no mesmo testemunho em que estava a fonte para a tradução espanhola dos excertos da obra de Vicente Ferrer, também presente no impresso sevilhano de 1497) e a da fonte latina (a fonte com a obra de Isaac estaria em latim). A análise de testemunhos supérstites da obra de Vicente Ferrer dos sécs. XV e XVI (manuscritos e impressos) não permitiu confirmar a hipótese da contiguidade material, embora se tenha obtido nova evidência de pertinência da hipótese. A análise de diversos testemunhos da tradição da obra de Isaac de Nínive (em grego, latim, italiano, francês, espanhol, catalão e português) permitiu não apenas confirmar a hipótese da fonte latina, como também evidenciou que ela está fortemente vinculada ao ramo do cód. 996 da Biblioteca Mazarina de Paris, de origem franciscana, embora este não tenha sido seu modelo.
Interpretação temporal de sentenças negativas em Karitiana
Authors: Ferreira Luiz Fernado
Published: October 2024

Este artigo apresenta um estudo sobre a interpretação temporal de orações negativas em Karitiana (ISO 639-3: ktn). O Karitiana é uma língua da família Arikém e tronco Tupi (RODRIGUES, 1986, p. 46) cujo sistema temporal é futuro vs. não-futuro (STORTO, 2002; MÜLLER; FERREIRA, 2020). Semanticamente, tempo é uma relação entre um momento de referência e momento da enunciação (KLEIN, 2013). Nessa língua, ele é expresso por sufixos verbais que estão presentes em orações afirmativas, mas ausentes em orações negativas (STORTO, 2018). Então, o objetivo deste estudo foi verificar como se dá a interpretação temporal nas negativas dada a ausência de morfologia de tempo nessas orações. Nossas hipóteses iniciais eram: (i) que a interpretação temporal é determinada contextualmente ou (ii) que ela é determinada gramaticalmente por outros fatores. Para testá-las, elaboramos um teste em duas etapas: (i) coleta contextualizada de dados de negativas tanto no futuro quanto no passado com um falante nativo e (ii) apresentação descontextualizada e aleatória desses áudios para outro falante nativo para tradução. Esse teste mostrou que a interpretação default de sentenças negativas descontextualizadas é o passado, mesmo que elas tenham sido coletadas em um contexto de futuro corroborando a segunda hipótese. A coleta de dados para análise semântica geralmente é feita de forma contextualizada (MATTHEWSON, 2004; SANCHEZ-MENDES, 2014). No entanto, mostramos como a tradução descontextualizada pode também ser uma ferramenta para detectar a existência de mecanismos gramaticais não salientes ao linguista determinando a interpretação.
O efeito da familiaridade como variável pragmática no processamento de metáforas
Authors: Kenedy Eduardo , Silva Gladiston
Published: October 2024

A convencionalidade é normalmente considerada a principal variável atuante no processamento de frases metafóricas. Neste artigo, defende-se, alternativamente, a hipótese de que é a familiaridade o fator pragmático determinante para a compreensão de metáforas nominais do tipo “X é um Y”. Autores como Searle (1979), Gibbs (1981), Janus e Bever (1985), Glucksberg (1988 e 2003), Bowdle e Gentner (2005) e Ricci (2016) utilizaram-se da convencionalidade como variável fundamental para os seus estudos experimentais, ignorando o efeito da familiaridade. É dentro deste contexto, e por contraste, que o presente estudo se insere. Um experimento on-line de leitura segmentada autocadenciada com frases metafóricas do tipo “X é um Y” apresentadas sem contexto prévio foi aplicado e revelou que a familiaridade foi a única variável que registrou efeito significativo.
Transformasi Desa Kunangan Melalui Website Literasi Kunangan.id Dengan Pemanfaatan Pojok Literasi Dalam Mengembangkan Minat Dan Baca
Authors: Bunga caroline
Published: October 2024

Indonesia saat ini memasuki era bonus demografi, sumber daya manusia(SDM) yang mumpuni sangat diperlukan jelang Indonesia Emas pada tahun 2045. Ada tiga aspek yang harus dipenuhi untuk mewujudkan SDM mumpuni, yaitu literasi dasar, karakter, dan kompetensi. Menjadi sebuah unit terkecil yang ada di pemerintahan, desa atau kelurahan dapat mendukung terciptanya smart village. Transformasi Digital pada desa harus diimbangin dengan peningkatan literasi masyarakat terhadap desa digital. Tujuan kegiatan ini adalah untuk dapat menunjang desa digital berkelanjutan dan meningkatkan minat baca masyarakat desa tanpa menghilangkan nilai-nilai sosial masyarakat era digital. Metode kegiatan ini dilakukan melalui studi kasus,survey, dokumentasi dan wawancara kepada beberapa tokoh masyarakat yang ada di Desa Kunangan. Dengan adanya transformasi digital melalui website Literasikunangan.id diharapkan tujuan-tujuan untuk tercapainya smart village.
La palabra escrita en el arte colonial
Authors: Atehortúa Atehortúa Arbey
Published: October 2024

El estudio sobre las relaciones entre pintura y código lingüístico en el arte colonial neogranadino, se concentra en la revisión de tres textos neogranadinos: la colección de retratos dedicados a las monjas muertas o monjas coronadas y las obras de Francisco Álvarez de Velasco y Pedro de Solís y Valenzuela. Otros textos coloniales son tenidos en cuenta como referencias para la investigación. En la serie de retratos sobre monjas hemos revisado la interacción entre la imagen y las cartelas. Para el tema de las imágenes en los libros y las otras posibilidades de relación entre poesía y pintura, se ha recurrido entonces a El desierto prodigioso y prodigio del desierto (1985) de Pedro de Solís y Valenzuela y A Rhythmica sacra, moral y laudatoria (1989) de Francisco Álvarez de Velasco. Estas dos extensas obras ofrecen una serie de posibilidades de relación entre lo pictórico y lo lingüístico, de tal modo que son emblemáticas del fenómeno en cuestión. El texto concluye con un capítulo en el cual se revisan las posibilidades de la pintura para la enseñanza de la Literatura. Como se trata de una propuesta didáctica, hemos ampliado el radio de acción, y se han incluido referencias a ciertas obras europeas.
Soluciones periódicas en ecuaciones diferenciales singulares
Authors: Gutiérrez Gutiérrez Alexander
Published: October 2024

Este libro es un compendio del trabajo de los últimos seis años y los modelos matemáticos tienen en común dos cosas: singularidades no lineales y coeficientes periódicos, se entiende por singularidad el límite al infinito del término no lineal cuando la variable de estado se acerca a un punto. El estudio de los modelos con singularidades es importante debido a que modelan procesos del mundo real que surgen en forma natural al considerar, por ejemplo, fuerzas gravitatorias, electromagnéticas o intermoleculares. En particular, centramos nuestra atención en el estudio de la existencia, unicidad y estabilidad de soluciones periódicas. Las herramientas matemáticas elegidas para abordar el estudio son amplias y comprende técnicas del análisis no lineal como son: teoremas de punto fijo, método de sub y super soluciones, y topológicas como: grado topológico, grado de coincidencia. Los resultados obtenidos se han logrado gracias a la colaboración de mis colegas: Profesor Pedro J. Torres de la Universidad de Granada y a los profesores Andrés M. Rivera y Daniel Núñez de la Universidad Javeriana, sede Cali.
1-hour postload glucose is a more sensitive marker of impaired β-cell functionthan 2-hour postload glucose
Authors: Lu Jingyi , Ni Jiaying , Su Hang , He Xingxing , Lu Wei , Zhu Wei , Wang Yufei , Ma Xiaojing , Bao Yuqian , Zhou Jian
Published: October 2024

Abstract There is evidence that 1-h plasma glucose (PG) during the 75-g oral glucose tolerance test (OGTT) is superior to 2-h PG in predicting diabetes. We aimed to investigate the characteristics of insulin sensitivity and β-cell function behind this observation. After age, sex and BMI matching, 496 subjects selected from 3965 non-diabetic individuals at high risk of type 2 diabetes in a tertiary medical center were categorized into four groups in a 1:1:1:1 ratio based on OGTT results: 1) 1 h < 8.6 mmol/L and 2 h < 7.8 mmol/L (normal glucose tolerance [NGT] /1h-normal); 2) 1 h ≥ 8.6 mmol/L and 2 h < 7.8 mmol/L (NGT/1h-high); 3) 1 h < 8.6 mmol/L and 2 h ≥ 7.8 mmol/L (impaired glucose tolerance [IGT]/1h-normal); and 4) 1 h ≥ 8.6 mmol/L and 2 h ≥ 7.8 mmol/L (IGT/1h-high). Compared with subjects with IGT/1h-normal, those with NGT/1h-high exhibited similar extent of insulin resistance but lower early-phase insulin secretion. Additionally, participants with NGT/1h-high had lower disposition index at both 0-30 min and 0-120 min than those with IGT/1h-normal. The fitted regression line relating PG to log-transformed disposition index (0-30 min and 0-120 min) was significantly steeper for 1-h than 2-h PG. In conclusion, 1-h PG seemed to be more sensitive to the deterioration in β-cell function than 2-h PG. The use of 1-h PG may identify individuals at high risk of type 2 diabetes at an earlier stage.

Article Highlights l

1-h plasma glucose (PG) was found to be superior to 2-h PG in predicting diabetes, but the mechanism remains to be clarified. l

What are the characteristics of insulin sensitivity and β-cell function in isolated defects of 1-h PG vs. 2-h PG? l Individuals with normal 2-h PG but elevated 1-h PG had similar insulin sensitivity but more impaired β-cell function, compared to those with elevated 2-h PG but normal 1-h PG.

1-h PG was more sensitive to β-cell function deterioration than 2-h PG. l

1-h PG may identify high-risk individuals for type 2 diabetes earlier than 2-h PG.
Autologous dermal fibroblasts stimulate regeneration of degenerative Achilles tendon
Authors: Volkova N. A , Yukhta M. S , Blonskiy R. I , Kostrub A. A , Goltsev A. N
Published: October 2024

Cell therapy with applying autologous skin fibroblasts could be an alternative or additional way to the standard therapy of tendon degenerative changes. The research was aimed to study a therapeutic effect of local administration of cultured and cryopreserved autologous skin fibroblasts in dynamics under tendopathy. Rats with modeled а^!^ tendon degenerative and dystrophic injury received by 0.25х10 6 of native or cryopreserved autologous skin fibroblasts into the defect area. The animals with administered physiological saline served as the control. 21 and 45 days after therapy there were implemented the histological, immunofluorescent and biomechanical studies. The data analysis testified to the fact, that cultured and cryopreserved autologous skin fibroblasts contributed to activating reparative processes in damaged tendons. On the first time point (21 day) the application of cryopreserved cells resulted in less pronounced therapeutic effect as compared to cultured fibroblasts. To the 45 day of study in аchilles tendons' histological structure of animals received both cultured and cryopreserved autologous skin fibroblasts we observed the presence of cell elements, reduction of disorganization phenomena, normalization of structure and position of collagen I type fibers. In addition, the animals' tendons with therapy by cultured and cryopreserved autologous skin fibroblasts had a positive dynamics in biomechanical indices: we observed a gradual positive strength recovery during tension, which to the 45 day of therapy did not statistically and significantly differ from the corresponding indices in the intact animals.
Experimental studyof titanium nickelideimplantsfor mandibula reconstruction
Authors: Dyuryagin N. M , Stepanov S. S , Semchenko V. V , Dyuryagina Ye. N
Published: October 2024

Reconstruction of the resectedmandiblefragmentsof rabbitsby NiTi implant leaded to bone restoration that occurred 4 times fasterthanunder naturalreparative processwithequal results. To minimize the effectof the implantonreparative processin the second casethe construction was fixedinpositionof the frontaldislocation, i.e.anteriorlyto the resectedbranchprojection. Long-termfunctional effect (62-65 months) in all casesjustifys the prospectsof medical devicefor clinical application.
Comparative analysis of osteogenic potential of multipotent mesenchymal stromal cells derived from oral mucosa and bone marrow
Authors: Zorin V. L , Zorina A. I , Eremin I. I , Bozo 1 I. Y , Solovieva E. V , Hromova N. V , Kopnin P. B
Published: October 2024

This article is focused on comparative analysis of osteogenic potential of multipotent mesenchymal stromal cells (MMSC) derived from different sources: gingival mucosa and bone marrow. MMSC immunophenotype, proliferation potential and osteogenic differentiation capacity were investigated in this work. Ability of MMSC adhesion to p-tricalcium phosphate was also examined. Osteoinductive potential of tissue-engineered bone grafts (MMSCs + p-tricalcium phosphate) was shown in experiments carried out on NOD-SCID mice in heterotopic bone formation test. Results of this research demonstrated that both type of MMSC had similar surface markers and osteogenic differentiation capacity. However MMSC derived from gingival mucosa were characterized by higher proliferative activity and higher adhesion level to p-tricalcium phosphate. Taking into consideration that MMSC could be obtained easier from gingival mucosa, this source of stem cells is probably the most promising for tissue engineering of bone grafts for stomatology and maxillofacial surgery (at least in case of investigated scaffold).
Programable biopolymeric microand nanocontainers for biocomputers
Authors: Erokhina S. , Pastorino L. , Sorokin V. , Erokhin V.
Published: October 2024

Polymeric capsules (containers) are widely used now in researches connected to the design of new pharmaceutical preparations. Here we present data allowing to consider these objects also as essential parts of biocomputers, capable, in particular, to carry out targeted delivery and triggered start of the «main program» (encapsulated specific molecules) to the areas with damaged functioning of the system, resulted from undesirable reactions. We also consider the use of cells as program package, capable to perform parallel functions in biocomputers when delivered in a proper place and started functioning in a proper moment.
Hyaluronan-based bioplastic material as a scaffold for biomedical cell-based express-product for skin regeneration
Authors: Kalmykova N. V , Spichkina O. G , Ellinidi V. N , Rakhmatullin R. R , Moiseev S. I
Published: October 2024

This study is designed to investigate the behavior of human fibroblasts and keratinocytes on hyaluronan-based bioplastic material «G-DERM». Cell viability, proliferation, interleikin-6 and keratinocytes growth factor secretion were investigated. The results suggested that «G-DERM» was not toxic and supported cell adhesion and spreading. Histological analysis showed that «G-DERM» properties and porous structure provided distribution of skin cells by cell-type manner. Fibroblasts on «G-DERM», being quiescent, kept their synthetic activity and produced tissue-specific factors of wound healing. According to study results «G-DERM» can be used as 3D cell matrix for regenerative medicine.
The action of Bacillus’ metzinc metalloproteinase on cultured animal cell lines
Authors: Danilova J. I , Kirillova J. M , Rudakova N. L , Bogomolnaya L. M , Balaban N. P , Sharipova M. R
Published: October 2024

Microbial enzymes with high activity are increasingly used in medical practice in development of drugs for treatment of burn injuries, diseases of gastrointestinal tract, as well as in systemic enzyme therapy. One of crucial requirements for such drugs is the absence of toxicity, both on cellular and organism levels. The aim of the present study was to evaluate cytotoxic effect of Bacillus pumilus metzinc metalloproteinase on animal cell lines. Cytological changes in LEK, Vero and PK-15 cell lines were observed after cell treatment with 155 ^/ml enzyme. However, lower enzyme concentrations - 75 and 15 ^/ml did not show any toxicity.
Features of senescence associated secretory phenotype (sasp) in gastrointestinal stromal tumors (GIsTs)
Authors: Ramazanov B. R , Boichuk S. V , Rizvanov A. A
Published: October 2024

To study the cellular senescence mechanisms and senescence-associated secretory phenotype of the tumor cell lines in response to doxorubicin exposure. The cell lines indicated above were used in present study: human fibroblasts lines BJ, human osteosarcoma cell line U-2 OS and gastrointestinal stromal tumor cell line GIST-T1. Genotoxic stress was induced by the transient exposure of doxorubicin (0.25 ug / ml) for 5 hours. DNA double-strand breaks formation was assessed by an increased expression and focal accumulation of the phosphorylated form of histone 2A (y-H2AX), whereas the activation of DNA repair pathway was assessed by focal accumulation of the phosphorylated form of ATM-kinase (pATM Ser1981). An increase of p-galactosidase activity was estimated by X-gal assay (5-bromo-4-chloro-3-indo-beta-D-galactoside). Interleukin-6 (IL-6) and interleukin-8 (IL-8) production was assessed by ELISA at 3, 6, 9, 12 days after 5h exposure to doxorubicin. Incubation of the tumor cell lines with doxorubicin induced DNA damage which triggered the DNA damage response (DDR) and activated a cellular senescence program associated with typical morphological changes, decrease of Lamin B1 expression and accumulation of p-galactosidase in the cytoplasm known as a traditional senescence markers. We observed a significant increase of IL-6 and IL-8 production in doxorubicin-treated human fibroblasts and tumor U-2 OS cells, whereas GIST-T1 cells secreted IL-6 only. In contrast to non-transformed cells, tumor cells after being exposed to doxorubicin triggered a permanent DNA damage, activation of cellular senescence program and production of proinflammatory cytokines (IL-6 and -8). Senescence-associated secretory phenotype (SASP) in GIST-T1 cells differs from the phenotype observed in the other of cancer cells and normal cells, as well. This might be taken into account during the development of the novel treatment options for the patients with gastrointestinal stromal tumors (GISTs).
Peribiliary glands of biliary tree as a niche of multipotent stem cells
Authors: Sharipova E. I , Gazizov I. M , Gumerova AA. A , Kiassov A. P
Published: October 2024

Peribiliary glands are located along the large bile ducts. They are found in human and majority of the animals. Other than mucous production, their function had not been defined until recently. But nowadays the question of their multiple functions is actively studied. Due to the last years reports peribiliary glands contain multipotent stem cells, which can differentiate into hepatocytes, cholangiocytes or pancreatic islets cells. The structure and function of peribiliary glands, known experimental models and perspectives of peribiliary glands use in regenerative medicine are discussed in the review.
Genetically encoded calcium indicator GCaMP6m for two-photon calcium imaging in newborn mouse cerebral cortex
Authors: Akhmetshina D. , Villette V. , Tressard T. , Malvache A. , Khazipov R. , Cossart R.
Published: October 2024

Calcium is a universal messenger regulating essential cellular signaling events in many tissues and organisms. In neurons, action potentials trigger large and rapid changes in cytoplasmic-free calcium concentration. Genetically encoded calcium indicators (GECIs) have been iteratively improved and are becoming useful for quantitative imaging of activity in defined neuronal populations in vivo. Among them, GCaMP6m has been recently introduced as an ultrasensitive calcium sensor for the experiments in vivo. Whether GECIs can be used in developmental studies to characterize early activity patterns in the immature cerebral cortex remains unknown, however. We used in vivo two-photon calcium imaging with GCaMP6m calcium indicator to characterize its developmental expression following adeno-associated virus (AAV) mediated viral induction of GCaMP6m expression in newborn mice (at postnatal days P0-2). Three days after virus injection, the vast majority of neurons showed fluorescent labeling in the neuronal cytoplasm, dendrites and axons. We found that the GCaMP6m calcium sensor is expressed in the cerebral cortex within 3-5 days following transfection at levels that enable monitoring spontaneous and sensory-evoked calcium transients in the intact mice barrel cortex in vivo. Thus, following GCaMP6m transfection at birth, it is possible to monitor neuronal populations in the barrel field of somatosensory cortex in P5-7 mice in vivo.
Physiological role of hydrogen sulfide in nervous system
Authors: Yakovlev A. V , Sitdikova G. F
Published: October 2024

The review provides modern data and the results of author's research on physiological and pathological roles of the new gasotransmitter - hydrogen sulfide (H2S) in the central and peripheral nervous system. H2S is synthesized by three enzymes: cystathionine p-syntase, cystathionine y-lyase and 3-mercaptopiruvat sulftransferase/cysteine aminotransferase. In nerve systems the main source of synthesis H2S is cystathionine p-syntase and high level enzyme expression observed in the embryonic and early postnatal period of organism development that is apparently necessary for the growth and maturation of neural networks for the protection of neurons and astrocytes in the conditions of oxidative stress. Cystathionine p-syntase gene mutation in humans leads to an autosomal recessive metabolic diseases, mental dysfunction, vascular lesions and hyperhomocysteinemia. The aim of this review is to present the currents data about the effects of H2S on ion channels, transmitter release, its participation in the pathology of various neurodegenerative diseases, as well as its antioxidative and neuroprotective action in central and peripheral nervous systems.
On the problem of lipid coding of genomic DNA: molecular dynamics peculiarities of DNA-phospholipid complexes
Authors: Zhdanov R. I , Aupov R. Kh , Andrianov G. V , Akberova N. I , Ibragimova M. Ya
Published: October 2024

Lipids can be bound to nucleic acids forming coding sequence at genomic DNA, and participating in realization of language of genome. Interaction of DNA oligonucleotide(A)20-(T)20 is studied with by moleculat dynamics method. The complex is prepared as a result of molecular docking, phospholipid molecule being located at DNA minor groove. Binding energy is equal to - 6.3 kcal/mol. Formation of hydrogen binding, interactions leading to those bonds, and some other interactions between DNA and phospholipid. The number of those interactions due to molecular dynamics is varied between 175 and 337. The key role in stabilization of DNA-phospholipid complex play also van-der-vaals and hydrophobic interactions along with hydrogen binding. The role of ethanolamine grouping in complexation between DNA and phospholipid is demonstrated.
Elimination of PKH26-labeled MMSC after allogeneic transplantation
Authors: Arutyunyan IV. V , Elchaninov A. V , Fatkhudinov T. H , Makarov A. V , Kananykhina E. Y , Bolshakova G. B , Glinkina V. V , Goldshtein D. V , Sukhikh G. T
Published: October 2024

The transplanted allogeneic multipotent mesenchymal stromal cells (MMSC) were previously thought to be poorly recognized by host immune system; the prolonged survival of these cells in host tissues was explained by their privileged immune status. As long as this concept is currently being revised, the understanding of MMSC routes should be reconsidered given the emerging role of host immune system in their gradual elimination. The study was focused upon elimination of PKH26-labeled MMSC, derived from umbilical cord, analyzed in animal models for two distinct pathologies: subtotal liver resection and critical skeletal muscle ischemia. Specific patterns of PKH26-positive macrophages (defined as CD68+ cells) were described for intact spleen and regenerating liver, and for the ischemic skeletal muscle, respectively. The PKH26-positive cells were observed in spleen of the subtotally hepatectomized model animals at 24 h. after surgery combined with MMSC transplantation; 83,2±4,6% of these were CD68+; the ratio reached 100% 3 days after transplantation. The PKH26-positive cells were also detected in regenerating liver starting from 3 days after transplantation, the great majority of them were CD68+ (96,8±2,2% and 96,3±2,6% for 3 and 10 days after transplantation, respectively). A different sort of host environment was provided by the damaged skeletal muscle model: productive phase of aseptic inflammation triggered by ischemia. The PKH26-positive fraction in the pool of macrophages significantly increased from 48,1 ±3,2% 3 days to 76,2±3,9% 30 days after transplantation. Thus, transplanted allogeneic MMSC are recognized and eliminated by host immune system. The rates of elimination depend on site of injection and time elapsed since the injection; the efficacy may reach 100%. The presence of РКН26 vital label (as well as any other exogenous label) in living cell can by no means solely prove its exogenous origin. The massive elimination of MMSC by host macrophages leads to impregnation of the latter with the dye that is masking the true presence of the former. The study accentuates the need of additional criteria for correct data interpretation.
Invasive ability of proteus mirabilis and morganella morganii
Authors: Zamaliutdinova N. M , Bogomolnaya L. B , Chastukhina I. B , Sharipova M. R , Mardanova A. M
Published: October 2024

Here we discuss the ability of Proteus mirabilis 5127-1 and Morganella morganii ZM to invade human cervical epithelial adenocarcinoma cells (HeLa-M) and to cause cytotoxicity in those cells. We found that ability of bacteria to invade HeLa-M cells depended on the multiplicity of infection for both strains. Cytotoxicity of P. mirabilis on HeLa-M cells was evaluated through quantification of eukaryotic cell detachment from plastic. We hypothesized that cytotoxicity of P.mirabilis could be due its extracellular proteolytic and hemolytic activities. In contrast, no cytotoxicity was detected in HeLa-M cells infected with M.morganii.
Proliferation of cells of the pancreas in experimental diabetes
Authors: Kaligin M. S , Titova AA. A , Plushkina A. S , Titova MA. A , Gumerova A. A , Kiassov A. P
Published: October 2024

The proliferative process intensification in the disrupted organs is very important in reparative regeneration. But the possibility of pancreas cells proliferation during damage is not been studied. The aim of our work was to examine the proliferation activity of pancreas during the experimental alloxan diabetes. The work was made on 33 rats. Blood glucose levels were measured. Also the expression of nuclear antigen of proliferating cells PCNA was studied in pancreas cells. After the day of the experimental hyperglycemia PCNA expression in islet and acinar cells of pancreas was found, which was persisted at all stages of the experiment. After 2 days of the experiment single PCNA-positive cells with the cytoplasmic staining were found in the interstitium, near vessels and ducts. Proliferating cells, which produce insulin were found in islets during double staining. Thus, there is the proliferation in islet and acinar cells of pancreas during experimental diabetes type I after alloxan damage. Also, the results of our study confirms the ability of p-cells and progenitor pancreas cells proliferation.
Controllable endogenic proteolysis as a major factor of switching tryptophanyl-tRNA synthetase from canonical aminoacylation activity to non-canonical regulatory functions
Authors: Nurbekov M. K , Elov A. A , Zhdanov R. I
Published: October 2024

Tryptophanyl-tRNA synthetase has a unique set of additional non-canonical activities in addition main aminoacylation activity, in particular for the control of angiogenesis [7]. It is important to study the role of endogenous limited controlled proteolysis as a possible molecular mechanism of switching from the canonical source aminoacylation activity of the native enzyme to non-canonical anti-angiogenic activity. We consider important data on the possible role of the significant for the activity zinc ion discovered earlier in the tryptophanyl-tRNA synthetase [8], and endogenous tryptophan in the activation of the regulatory functions of the enzyme. We have developed a novel approach to the identification of the role of endogenous proteolysis induced by intracellular proteases in the modification of the enzyme, as well as the role of the endogenous enzyme bound tryptophan and varying content of zinc ion essential for tryptophanyl-tRNA synthetase activity, as important factors in the complex process of activation of the non-canonical cytokine functions of the enzyme. Proteolytic transition of native tryptophanyl-tRNA synthetase as a result of above modifications of the enzyme and resulting cleavage of N-terminal fragment of 20 kDa have been shown with chromatographic, immunochemical methods, and confirmed by electrophoresis and immunoblotting.
Transplantation of mononuclear cells of human umbilical cord blood improves spatial memory in APP/PS1 transgenic mice with alzheimer's disease model
Authors: Petukhova E. O , Mukhamedshina Y. O , Rizvanov A. A , Mukhitov A. R , Zefirov A. L , Islamov R. R , Mukhamedyarov M. A
Published: October 2024

Alzheimer's disease is progressive incurable neurodegenerative disease, which is manifested mainly by dementia. One of the most promising directions in development of Alzheimer's disease treatment is use of gene-cell technologies. The aim of current study was to perform transplantation of wild-type or EGFP expressing umbilical cord blood mononuclear cells (МКПК) to APP/PS1 transgenic mice with Alzheimer's disease model with further evaluation of transplantation impact with behavioral (T-maze, plus maze, open field) and immunohistochemical methods. It was found that МКПК transplantation significantly ameliorates behavioral performance of APP/PS1 mice: improves spatial memory, decreases anxiety and non-specific excitability, increases the efficacy of exploratory behavior. Grafted cells were found in cortex and hippocampus of mice even 3 months after МКПК transplantation, herewith EGFP expression in grafted cells was found at early stages after transplantation. Thus, use of МКПК-based gene-cell constructs represents a promising direction in development of Alzheimer's disease therapy.
Development and applications of a new technique to study blood clot contraction (retraction)
Authors: Lozhkin A. P , Peshkova A. D , Ataullakhanov F. I , Litvinov R. I
Published: October 2024

Despite the importance for hemostasis and thrombosis, platelet-governed clot shrinkage has not been systematically studied, partially due to the lack of methodology to follow and quantify clot contraction dynamics. We have developed a new technique based on the continuous tracking of clot size. An optical platform for this method is the commercially available Thrombodynamics Analyser System (HemaCore, Russia). A standard procedure includes recalcification of whole citrated blood and addition of thrombin to initiate blood clotting and platelet activation. The clot contraction is monitored by taking images every 15 seconds over 20 minutes or more followed by off-line computational analysis that provides a kinetic curve characterized by 6 numerical parameters. Clot contraction has at least two phases characterized by distinct rates. Exogenous Ca2+ is not indispensable for clot contraction to occur; however, the clots formed without addition of Ca2+ were less stable. Recalcification of blood with 2-5 mM [Ca2+] prevented the red blood cell fallout without an effect on the contraction kinetics. 10 mM [Ca2+] partially inhibited clot contraction. Iodoacetamide, an inhibitor of factor XIIIa, did not affect clot formation but abolished clot shrinkage, confirming that factor XIIIa is essential for clot contraction. Thrombin enhanced the rate and degree of clot contraction in a dose-dependent manner. Blood clot contraction was substantially delayed in patients on warfarin compared to healthy donors. We developed an accurate and simple assay for blood clot contraction which can be used for research and may be potentially useful for in vitro diagnostics.
The influence of P2-receptors on the cytotoxic effect of cisplatine in squamous cell uterine cervix cancer in vitro
Authors: Raginov I. S , Muhamedyarova G. K , Panov A. V
Published: October 2024

To investigate the influence of purinergic signalling in squamous cell uterine cervix cancer in vitro, the effect of combining ATP (nonselective agonist P2-receptor) with the nonselective P2 receptor antagonists PPADS (P2X-receptors), RB2 (P2Y-receptors) and suramin (both P2Y-receptors and P2X-receptors) and the chemotherapeutic drug cisplatin was assessed. ATP effectively reduces up to twofold the growth of uterine cervix cancer cells. RB2 promoted survival of tumor cell by 52,1% compared to the control. Combinations of the cisplatin + ATP demonstrated a strong sensitivity at 2,7 fold. However, this effect of ATP was reversed in the presence of RB2 by 41%. This highlights the potential use of selective P2Y receptor agonists in the treatment of squamous cell uterine cervix cancer.
Pathohistological assessment of skeletal muscle after direct gene therapy with vegf165 of patients with peripheral arterial diseases
Authors: Mavlikeev M. O , Plotnikov M. V , Maksimov A. V , Gafiyatullina G. R , Murtazin A. I , Teregulov U. E , Shamsutdinova I. I , Gumerova A. A , Rizvanov A. A , Kiassov A. P
Published: October 2024

The aim was to elucidate impact of gene therapy with plasmid encoding vegf165 on the muscle tissue pathohistology of patients with peripheral arterial diseases. Twice repeated intramuscular injections of plasmid («Neovaskulgen», RN LP-000671 from 28.09.2011) were performed to 6 patients (ischemia grade IIb-III by Pokrovsky-Fontaine) according to specification. Standart tredmill test, ankle-brachial index estimation were performed. Histological study of injured muscle biopsies taken before and 3 months after injection was performed. In intact muscles therapy with vegf165 leads to increase of mean cross-sectional muscle fiber area without significant angiogenic effect. In muscles with decreased capillary density this therapy leads to blood supply improvement promoting regeneration of muscles by myosatellites proliferation and increase of mean cross-sectional muscle fiber area and connective tissue degradation. Treadmill test showed painless walking distance increased by 31,74% on average (from 94,96±49,79 m to 139,11±60,78 m, p
Muscarinic regulation of calcium transient and synaptic transmission in frog neuromuscular junction
Authors: Samigullin D. V , Khaziev E. F , Kovyazina I. V , Bukharaeva E. A , Nikolsky E. E
Published: October 2024

In frog neuromuscular junction, muscarine, exogeneous acetylcholine and acetylcholinesterase inhibitor proserine reduced the intensity of calcium-sensitive dye fluorescence (calcium transient) at low frequency of nerve stimulation, suggesting that calcium ions entry into nerve ending was decreased. M2 muscarinic receptor blocker methoctramine prevented the action of muscarine. The amplitude of endplate currents was reduced in presence of muscarine at low frequency nerve stimulation, and atropine abolished this effect. Amplitudes of endplate currents evoked by high frequency stimulation were enhanced in presence of methoctramine, and synaptic depression was less pronounced, probably due to elevated calcium concentration in nerve ending. Thus, activation of presynaptic muscarinic receptors predominantly of M2 subtype reduces the intensity of quantal acetylcholine release in frog neuromuscular synapses that may be associated with decreased level of calcium ions in the nerve ending to provide the modulation of postsynaptic currents amplitude at high frequency firing.
Deriving population of ectomesenchymal cells with properties of stem cells and progenitor cells from pulp of permanent teeth
Authors: Sagitov I. I , Shafigullina A. K , Saleeva G. T , Gomzikova M. O , Rizvanov AA. A , Kiassov A. P
Published: October 2024

Lately a lot of research has been focused on isolation of tooth stem cells. Our earlier studies showed that odontoblasts have a number of features typical for ectodermal cells, such as expression of epithelial membrane antigen (EMA) and cytokeratin 18. Aim: to obtain populations of ectomesenchymal cells from permanent teeth pulp with properties of stem and progenitor cells and characterize their phenotype in vitro. The pulp of permanent teeth of 15 patients (age 18- 29) was removed according to the orthodontic indications and used for stem cells isolation. The phenotype of derived cells was studied using the immunocytochemical staining method. Antibody staining for EMA, C-kit, cytokeratine 19, ESA, aSMA, Desmin, Vimentin, PCNA, Bcl-2. EMA and cytoceratine 18 expression was also confirmed by western blotting. The immunophenotype of obtained stem cells was determined by flow cytometry Significant expression of C-kit and Vimentin was observed at all times of cultivation as demonstrated by immunocytochemical staining. About a half of cells expressed EMA on all passages. First and second passages cells were characterized by expression of cytoceratine 18 which gradually decreased and to the fifth passage only single cells were positively stained. EMA and cytokeratin 18 expression was confirmed by western blotting. Flow cytometry revealed that cells obtained by us from human permanent teeth pulp were positive for CD29, CD90, CD10, CD54, CD56, CD166 and negative for CD14, CD34, CD45.
H. pylori, mikrobiota kishechnika, antibiotikorezistentnost': est' li vzaimosvyaz'?
Authors: Safina D. D , Abdulkhakov S. R , Abdulkhakov R. A , Ismagilova R. K , Tyakht A. V , Popenko A. S
Published: October 2024

Helicobacter pylori (H. pylori) eradication is obligatory in case of such diseases as gastric and duodenal ulcer, atrophic gastritis, MALT lymphoma, in patients after gastrectomy due to the gastric cancer, as well as in close relatives of gastric canсer patients. Antibacterial therapy which is aimed at the eradication of H. pylori has an impact on the intestinal microbiotа leading to its qualitative and quantitative disturbances. The review focuses on the influence of H. pylori and eradication therapy on the content of the intestinal microbiota, their role in the development of intestinal microbiota resistance to antibiotics. Both H. pylori itself and eradication therapy can lead to the changes of gut microbiota content, which are characterized by reduction in microorganisms diversity; decrease in the number of obligate anaerobes, prevailing in normal gut; increase in the number of facultative aerobic microorganisms. Long-term effect of antibiotic therapy is the development of antibiotic-resistant strains of bacteria. Resistance may be due to the presence of the resistance genes in bacteria, which they have acquired through mutations or horizontal transfer. These genes can be spread not only among pathogenic bacteria, due to the presence of which antibacterial therapy is carried out, but also among the members of the normal microflora, which can then be modified and loose its beneficial properties. Thus the intestinal microflora may serve as a potential reservoir of resistance genes and contribute to the development of antibiotics resistance through other members of the human microbiota.
The action of bacillary proteases on matrix metalloproteinases of different cell lines
Authors: Toymentseva A. A , Danilova Y. V , Balaban N. P , Mardanova A. M , Sharipova M. R
Published: October 2024

It was investigated the effect of secreted proteinases Bacillus pumilus 7P belonging to different classes of proteolytic proteins on matrix metalloproteinases of mouse fibroblast (3T3BalbSV40), multipotent mesenchymal stromal and cells Hela-M. Matrix metalloproteinases of all cell lines was inactivated by glutamyl-specific endopeptidase. Subtilisin-like proteinase and adams-like metalloproteinase of B. pumilus did not change the activity of matrix metalloproteinases of differentcell lines.