bluuebunny/crossref_metadata_2025 huggingface dataset:
ANALISA CAMPURAN METANOL–PERTALITE TERHADAP KINERJA DAN SUHU KERJA MOTOR
Authors: Susilo Sugeng Hadi , Suharono M. Fanny , Rarindo Hari , Wicaksono Hangga
Published: November 2020

The study aims to determine the effect of using a mixture of methanol add on to pertalite to the performance of gasoline engines, especially injection system with a capacity of 250 cc engine with two cylinders. This research used experimental method with variables used in this research is mixture of pure pertalite (P), mixture of pertalite - methanol 90% - 10% (PM1), mixture of pertalite - methanol 80% - 20% (PM2), mixture of pertalite - methanol 70% - 30% (PM3), mixed pertalite - methanol 60% - 40% (PM4), 50% - 50% (PM5) pertalite - methanol mixtures, pertalite - methanol 40% - 60% (PM6). Variable related in this research is power, torque and working temperature of the engine. The results of this study indicate that the addition of methanol with pertalite fuel tends to produce lower power and torque, while the temperature of the radiator and exhaust system shows that the use of methanol mixture with pertalite fuel can significantly increase the engine temperature of the vehicle.
Efficacy of Etanercept with Jaw - Training for Rheumatoid Arthritis with Firstly Temporomandibular Joint Symptoms: A Case Report
Authors: Kashiwagi Miki , Abe Takahiro , Komiyama Yuske , Komatsu Noriko , Tateishi Shoko , Hoshi Kazuto
Published: November 2020

Some patients with Rheumatoid Arthritis suffer with Temporomandibular Disorders (TMD). A 36-year-old Asian female presented to our hospital with Temporomandibular Joint (TMJ) symptoms, including pain. The maximum self-opening distance was 23 mm at first visit. As symptoms were worse in the morning and also started occurring in other joints, rheumatologists were consulted. The rheumatologists prescribed Etanercept (ETN), a tumor necrosis factor inhibitor which is commonly used in the early stages of Rheumatoid Arthritis (RA). The use of ETN together with conservative therapy for the TMJ improved the symptoms in the affected joints. No therapy for TMJ symptoms in RA patients has yet been established. However, the findings of this case suggest that the usage of biological products from the early stage of RA and jaw-training may be possible to manage TMJ symptoms.
Performance Improvement for Detecting Brain Function Using fNIRS: A Multi-Distance Probe Configuration With PPL Method
Authors: Chen Xinrui , Song Xizi , Chen Long , An Xingwei , Ming Dong
Published: November 2020

To improve the spatial resolution of imaging and get more effective brain function information, a multi-distance probe configuration with three distances (28.2, 40, and 44.7 mm) and 52 channels is designed. At the same time, a data conversion method of modified Beer–Lambert law (MBLL) with partial pathlength (PPL) is proposed. In the experiment, three kinds of tasks, grip of left hand, grip of right hand, and rest, are performed with eight healthy subjects. First, with a typical single-distance probe configuration (30 mm, 24 channels), the feasibility of the proposed MBLL with PPL is preliminarily validated. Further, the characteristic of the proposed method is evaluated with the multi-distance probe configuration. Compared with MBLL with differential pathlength factor (DPF), the proposed MBLL with PPL is able to acquire more obvious concentration change and can achieve higher classification accuracy of the three tasks. Then, with the proposed method, the performance of the multi-distance probe configuration is discussed. Results show that, compared with a single distance, the combination of the three distances has better spatial resolution and could explore more accurate brain activation information. Besides, the classification accuracy of the three tasks obtained with the combination of three distances is higher than that of any combination of two distances. Also, with the combination of the three distances, the two-class classification between different tasks is carried out. Both theory and experimental results demonstrate that, using multi-distance probe configuration and the MBLL with PPL method, the performance of brain function detected by NIRS can be improved.
Associations of Platelet Count with Inflammation and Response to Anti-TNF-α Therapy in Patients with Ankylosing Spondylitis
Authors: Qian Hongyan , Chen Rongjuan , Wang Bin , Yuan Xiaoqing , Chen Shiju , Liu Yuan , Shi Guixiu
Published: November 2020

Background: Increased platelet count has been reported in ankylosing spondylitis (AS) patients, but its clinical significance is still largely elusive. The objective of this study was to evaluate the clinical role of platelet count in AS patients, especially its impact on treatment outcomes.

Methods: A case-control study containing 35 AS patients receiving anti-tumor necrosis factor-α (anti-TNF-α) therapy and 45 healthy controls was performed, and AS patients were followed at least 6 months after anti-TNF-α therapy. A systematic review and meta-analysis of studies containing relevant data on outcomes of interest was also performed.

Results: AS patients had significantly higher platelet count than controls (p = 0.0001), and the significantly increased platelet count in AS patients was confirmed in a meta-analysis of 14 studies involving 1,223 AS patients and 913 controls (mean difference = 39.61, 95% CI 27.89–51.34, p < 0.001). Besides, platelet count was significantly correlated with ESR (p < 0.001) and was moderately correlated with ASDAS-CRP score (p = 0.002). Moreover, anti-TNF-α therapy could reduce platelet count in AS patients at the first month and the effect was maintained through the treatment duration. In the prospective follow-up study of those 35 AS patients, those responders to anti-TNF-α therapy had significantly lower platelet count than nonresponders (p = 0.015). Logistic regression analysis suggested that lower platelet count was associated with higher possibility of achieving good response to anti-TNF-α therapy in AS patients (odds ratio = 2.26; 95% CI = 1.06–4.82; p = 0.035).

Conclusion: This study suggested that platelet count was associated with inflammation severity and treatment outcomes in AS patients, and elevated platelet count was a promising biomarker of poorer response to anti-TNF-α therapy. The findings above need to be validated in more future studies.
Celebrex Adjuvant Therapy on Coronavirus Disease 2019: An Experimental Study
Authors: Hong Wenxin , Chen Yan , You Kai , Tan Shenglin , Wu Feima , Tao Jiawang , Chen Xudan , Zhang Jiaye , Xiong Yue , Yuan Fang , Yang Zhen , Chen Tingting , Chen Xinwen , Peng Ping , Tai Qiang , Wang Jian , Zhang Fuchun , Li Yin-Xiong
Published: November 2020

Background:The pandemic of coronavirus disease 2019 (COVID-19) resulted in grave morbidity and mortality worldwide. There is currently no effective drug to cure COVID-19. Based on analyses of available data, we deduced that excessive prostaglandin E2(PGE2) produced by cyclooxygenase-2 was a key pathological event of COVID-19.

Methods:A prospective clinical study was conducted in one hospital for COVID-19 treatment with Celebrex to suppress the excessive PGE2production. A total of 44 COVID-19 cases were enrolled, 37 cases in the experimental group received Celebrex as adjuvant (full dose: 0.2 g,bid; half dose: 0.2 g,qd) for 7–14 days, and the dosage and duration was adjusted for individuals, while seven cases in the control group received the standard therapy. The clinical outcomes were evaluated by measuring the urine PGE2levels, lab tests, CT scans, vital signs, and other clinical data. The urine PGE2levels were measured by mass spectrometry. The study was registered and can be accessed athttp://www.chictr.org.cn/showproj.aspx?proj=50474.

Results:The concentrations of PGE2in urine samples of COVID-19 patients were significantly higher than those of PGE2in urine samples of healthy individuals (mean value: 170 ng/ml vs 18.8 ng/ml,p< 0.01) and positively correlated with the progression of COVID-19. Among those 37 experimental cases, there were 10 cases with age over 60 years (27%, 10/37) and 13 cases (35%, 13/37) with preexisting conditions including cancer, atherosclerosis, and diabetes. Twenty-five cases had full dose, 11 cases with half dose of Celebrex, and one case with ibuprofen. The remission rates in midterm were 100%, 82%, and 57% of the full dose, half dose, and control group, respectively, and the discharged rate was 100% at the endpoint with Celebrex treatment. Celebrex significantly reduced the PGE2levels and promoted recovery of ordinary and severe COVID-19. Furthermore, more complications, severity, and death rate were widely observed and reported in the COVID-19 group of elders and with comorbidities; however, this phenomenon did not appear in this particular Celebrex adjunctive treatment study.

Conclusion:This clinical study indicates that Celebrex adjuvant treatment promotes the recovery of all types of COVID-19 and further reduces the mortality rate of elderly and those with comorbidities.
Quantitative Hormone Signaling Output Analyses of Arabidopsis thaliana Interactions With Virulent and Avirulent Hyaloperonospora arabidopsidis Isolates at Single-Cell Resolution
Authors: Ghareeb Hassan , El-Sayed Mohamed , Pound Michael , Tetyuk Olena , Hanika Katharina , Herrfurth Cornelia , Feussner Ivo , Lipka Volker
Published: November 2020

The phytohormones salicylic acid (SA), jasmonic acid (JA), and ethylene (ET) are central regulators of biotic and abiotic stress responses in Arabidopsis thaliana. Here, we generated modular fluorescent protein-based reporter lines termed COLORFUL-PR1pro, -VSP2pro, and -PDF1.2apro. These feature hormone-controlled nucleus-targeted transcriptional output sensors and the simultaneous constitutive expression of spectrally separated nuclear reference and plasma membrane-localized reporters. This set-up allowed the study of cell-type specific hormone activities, cellular viability and microbial invasion. Moreover, we developed a software-supported high-throughput confocal microscopy imaging protocol for output quantification to resolve the spatio-temporal dynamics of respective hormonal signaling activities at single-cell resolution. Proof-of-principle analyses in A. thaliana leaves revealed distinguished hormone sensitivities in mesophyll, epidermal pavement and stomatal guard cells, suggesting cell type-specific regulatory protein activities. In plant-microbe interaction studies, we found that virulent and avirulent Hyaloperonospora arabidopsidis (Hpa) isolates exhibit different invasion dynamics and induce spatio-temporally distinct hormonal activity signatures. On the cellular level, these hormone-controlled reporter signatures demarcate the nascent sites of Hpa entry and progression, and highlight initiation, transduction and local containment of immune signals.
Appraising the Welfare of Thoroughbred Racehorses in Training in Queensland, Australia: The Incidence and Type of Musculoskeletal Injuries Vary between Two-Year-Old and Older Thoroughbred Racehorses
Authors: Crawford Kylie L. , Finnane Anna , Greer Ristan M. , Phillips Clive J. C. , Woldeyohannes Solomon M. , Perkins Nigel R. , Ahern Benjamin J.
Published: November 2020

Musculoskeletal injuries (MSI) remain a concerning cause of racehorse morbidity and mortality with important ethical and welfare consequences. Previous research examining risk factors for MSI report inconsistent findings. Age is thought to affect MSI risk, but, to date, there have been no prospective studies comparing MSI in two-year-old versus older horses. This study aimed to: (1) determine the incidence of MSI for two-year-old and older horses, and whether this was affected by training track, season, or rainfall, and (2) determine the types of MSI affecting two-year-old and older horses, and whether horses trialled or raced after injury. A prospective survey was conducted with data collected through personal structured weekly interviews with participating trainers over a 13-month period. Data were analysed using Poisson regression. The incidence of MSI in the current study was low (0.6%). The incidence of MSI in two-year-old horses was higher than older horses (p < 0.001). Types of MSI varied between two-year-old and older horses (p < 0.001) and affected whether horses subsequently trailed or raced from 11 to 23 months after injury (p < 0.001). A larger proportion of two-year-old horses had dorsal metacarpal disease and traumatic lacerations. A smaller proportion of two-year-old horses had suspensory ligament desmitis, superficial digital flexor tendonitis, proximal sesamoid bone fractures, and fetlock joint injuries than older horses. Training track and rainfall did not affect MSI. The season affected MSI in two-year-old horses (p < 0.001) but not older horses. The major limitation was that trainers in this study were metropolitan (city) and our findings may not be generalisable to racehorses in regional (country) areas. Another significant limitation was the assumption that MSI was the reason for failure to trial or race after injury. In conclusion, the incidence of MSI was low in the current study and the types and the risk factors for MSI are different for two-year-old and older horses.
Effects of Low ω6:ω3 Ratio in Sow Diet and Seaweed Supplement in Piglet Diet on Performance, Colostrum and Milk Fatty Acid Profiles, and Oxidative Status
Authors: Nguyen Thi Xuan , Agazzi Alessandro , Comi Marcello , Bontempo Valentino , Guido Invernizzi , Panseri Sara , Sauerwein Helga , Eckersall Peter David , Burchmore Richard , Savoini Giovanni
Published: November 2020

The ratio of omega-6 (ω6) to omega-3 (ω3) polyunsaturated fatty acids (PUFAs) in the diet contributes to animal health and performance modulations because they have mostly opposite physiological functions. Increasing ω3 PUFAs content in the maternal diet can stimulate antioxidative capacity in sow and piglets; however, the optimal ratio of ω6 and ω3 PUFAs in the sow diet is still under discussion. Rich sources of bioactive constituents such as brown seaweed are an excellent supplementation to promote animal health and antioxidant status. However, the knowledge of the effects of this compound, specifically in post-weaning piglets, is still limited. Moreover, the combined effect of a low ω6:ω3 PUFAs ratio in sow diet and seaweed supplementation in post-weaning piglets’ diet has never been studied. This research aims to assess the combined effect of a low ω6:ω3 ratio in sow diets and seaweed supplementation in piglet diets on their growth and oxidative status. We also assessed the impact of a low ω6:ω3 ratio in the maternal diet on reproduction, milk fatty acid (FA) profile, and plasma leptin concentration. Two sow diets (n = 8 each) contained either a control ratio (CR, 13:1 during gestation, starting from day 28 (G28) and 10:1 during lactation) or a low ratio (LR, 4:1 from G28 until the end of lactation (L-End)) of ω6:ω3 FA by adding soybean oil or linseed oil, respectively. Reproductive performance was evaluated. Colostrum and milk at lactation day 7 (L7) and L-End were collected to analyze FA profile. Plasma was collected at G28, G79, G108, L7, L14, and L-End for determination of leptin and oxidative status. At weaning, 20 male piglets were selected per sow group to form 4 diet treatments (n = 10 each), which were supplemented with or without 4 g/kg seaweed. Recording of growth performance and collection of blood were performed at days 0, 7, 15, and 21 of post-weaning for oxidative status. LR diet increased (p < 0.05) the survival rate of piglets at weaning, and individual and litter weight gains. Colostrum and milk at L7 and L-End had lower (p < 0.05) ω6:ω3 ratio in LR sows. Interaction between dietary treatments on sows and piglets was revealed for all examined growth parameters at most time points (p < 0.05). LR diet did not affect plasma leptin levels and oxidative status. These findings suggest that the seaweed supplement during post-weaning could not improve growth rate and oxidative status of piglets born from mothers receiving a low dietary ω6:ω3 ratio (4:1) during gestation and lactation. However, this low ratio was beneficial for weaning survival rate, sucking piglets’ weight gain, and ω3 enrichment in colostrum and milk.
Assessment of the Growth and Reproductive Performance of Cloned Pietrain Boars
Authors: Shi Junsong , Tan Baohua , Luo Lvhua , Li Zicong , Hong Linjun , Yang Jie , Cai Gengyuan , Zheng Enqin , Wu Zhenfang , Gu Ting
Published: November 2020

How to maximize the use of the genetic merits of the high-ranking boars (also called superior ones) is a considerable question in the pig breeding industry, considering the money and time spent on selection. Somatic cell nuclear transfer (SCNT) is one of the potential ways to answer the question, which can be applied to produce clones with genetic resources of superior boar for the production of commercial pigs. For practical application, it is essential to investigate whether the clones and their progeny keep behaving better than the “normal boars”, considering that in vitro culture and transfer manipulation would cause a series of harmful effects to the development of clones. In this study, 59,061 cloned embryos were transferred into 250 recipient sows to produce the clones of superior Pietrain boars. The growth performance of 12 clones and 36 non-clones and the semen quality of 19 clones and 28 non-clones were compared. The reproductive performance of 21 clones and 25 non-clones were also tested. Furthermore, we made a comparison in the growth performance between 466 progeny of the clones and 822 progeny of the non-clones. Our results showed that no significant difference in semen quality and reproductive performance was observed between the clones and the non-clones, although the clones grew slower and exhibited smaller body size than the non-clones. The F1 progeny of the clones showed a greater growth rate than the non-clones. Our results demonstrated through the large animal population showed that SCNT manipulation resulted in a low growth rate and small body size, but the clones could normally produce F1 progeny with excellent growth traits to bring more economic benefits. Therefore, SCNT could be effective in enlarging the merit genetics of the superior boars and increasing the economic benefits in pig reproduction and breeding.
Bringing the Heavy Chain to Light: Creating a Symmetric, Bivalent IgG-Like Bispecific
Authors: Ramasubramanian Anusuya , Tennyson Rachel , Magnay Maureen , Kathuria Sagar , Travaline Tara , Jain Annu , Lord Dana M. , Salemi Megan , Sullivan Caitlin , Magnay Tristan , Hu Jiali , Bric-Furlong Eva , Rival Pierrick , Zhou Yanfeng , Hoffmann Dietmar , Brondyk William , Radošević Katarina , Chowdhury Partha S.
Published: November 2020

Bispecific molecules are biologically significant, yet their complex structures pose important manufacturing and pharmacokinetic challenges. Nevertheless, owing to similarities with monoclonal antibodies (mAbs), IgG-like bispecifics conceptually align well with conventional expression and manufacturing platforms and often exhibit potentially favorable drug metabolism and pharmacokinetic (DMPK) properties. However, IgG-like bispecifics do not possess target bivalency and current designs often require tedious engineering and purification to ensure appropriate chain pairing. Here, we present a near-native IgG antibody format, the 2xVH, which can create bivalency for each target or epitope and requires no engineering for cognate chain pairing. In this modality, two different variable heavy (VH) domains with distinct binding specificities are grafted onto the first constant heavy (CH1) and constant light (CL) domains, conferring the molecule with dual specificity. To determine the versatility of this format, we characterized the expression, binding, and stability of several previously identified soluble human VH domains. By grafting these domains onto an IgG scaffold, we generated several prototype 2xVH IgG and Fab molecules that display similar properties to mAbs. These molecules avoided the post-expression purification necessary for engineered bispecifics while maintaining a capacity for simultaneous dual binding. Hence, the 2xVH format represents a bivalent, bispecific design that addresses limitations of manufacturing IgG-like bispecifics while promoting biologically-relevant dual target engagement.
Abstracting Strings for Model Checking of C Programs
Authors: Lauko Henrich , Olliaro Martina , Cortesi Agostino , Roc̆kai Petr
Published: November 2020

Data type abstraction plays a crucial role in software verification. In this paper, we introduce a domain for abstracting strings in the C programming language, where strings are managed as null-terminated arrays of characters. The new domain M-String is parametrized on an index (bound) domain and a character domain. By means of these different constituent domains, M-Strings captures shape information on the array structure as well as value information on the characters occurring in the string. By tuning these two parameters, M-String can be easily tailored for specific verification tasks, balancing precision against complexity. The concrete and the abstract semantics of basic operations on strings are carefully formalized, and soundness proofs are fully detailed. Moreover, for a selection of functions contained in the standard C library, we provide the semantics for character access and update, enabling an automatic lifting of arbitrary string-manipulating code into our new domain. An implementation of abstract operations is provided within a tool that automatically lifts existing programs into the M-String domain along with an explicit-state model checker. The accuracy of the proposed domain is experimentally evaluated on real-case test programs, showing that M-String can efficiently detect real-world bugs as well as to prove that program does not contain them after they are fixed.
Parameter Analysis and Optimization of Annular Jet Pump Based on Kriging Model
Authors: Xu Kai , Wang Gang , Wang Liquan , Yun Feihong , Sun Wenhao , Wang Xiangyu , Chen Xi
Published: November 2020

Jet pump efficiency heavily relies on the geometrical parameters of the pump design and parameter global optimization in the full variable space is still a big challenge. This paper proposed a global optimization method for annular jet pump design combining computational fluid dynamics (CFD) simulation, the Kriging approximate model and experimental data. The suction angle, the flow ratio, the diffusion angle, and the area ratio are selected as the design variables for optimization. The optimal space filling design (OSF) method is used to generate sampling points from the design space of the four design variables. The optimization method solves the constrained optimization problem with a given head ratio by building the functional relationship established by the Kriging model between efficiency and design parameters, which makes the method more applicable. The design result shows that the annular jet pump efficiency is predicted well by the Kriging model; m is a key variable affecting the annular jet pump efficiency. As the area ratio m decreases, the mixing effect at the suction chamber outlet can be improved, but the frictional resistance increases.
Deep Neural Network-Based Guidance Law Using Supervised Learning
Authors: Kim Minjeong , Hong Daseon , Park Sungsu
Published: November 2020

This paper proposes that the deep neural network-based guidance (DNNG) law replace the proportional navigation guidance (PNG) law. This approach is performed by adopting a supervised learning (SL) method using a large amount of simulation data from the missile system with PNG. Then, the proposed DNNG is compared with the PNG, and its performance is evaluated via the hitting rate and the energy function. In addition, the DNN-based only line-of-sight (LOS) rate input guidance (DNNLG) law, in which only the LOS rate is an input variable, is introduced and compared with the PN and DNNG laws. Then, the DNNG and DNNLG laws examine behavior in an initial position other than the training data.
Multi-Objective Optimization of Production Objectives Based on Surrogate Model
Authors: Červeňanská Zuzana , Kotianová Janette , Važan Pavel , Juhásová Bohuslava , Juhás Martin
Published: November 2020

The article addresses an approximate solution to the multi-objective optimization problem for a black-box function of a manufacturing system. We employ the surrogate of the discrete-event simulation model of a batch production system in an analytical form. Integration of simulation, Design of Experiments methods, and Weighted Sum and Weighted Product multi-objective methods are used in an arrangement of a priori defined preferences to find a solution near the Pareto optimal solution in a criterion space. We compare the results obtained through the analytical approach to the outcomes of simulation-based optimization. The observed results indicate a possibility to apply the suitable analytical model for quickly finding the acceptable approximate solution close to the Pareto optimal front.
Nxt-Freedom: Considering VDC-Based Fairness in Enforcing Bandwidth Guarantees in Cloud Datacenter
Authors: Wang Shuo , Zhou Zhiqiang , Zhang Hongjie , Li Jing
Published: November 2020

In the cloud datacenter, for the multi-tenant model, network resources should be fairly allocated among VDCs (virtual datacenters). Conventionally, the allocation of cloud network resources is on a best-effort basis, so the specific information of network resource allocation is unclear. Previous research has either aimed to provide minimum bandwidth guarantee, or focused on realizing work conservation according to the VM-to-VM (virtual machine to virtual machine) flow policy or per-source policy, or both policies. However, they failed to consider allocating redundant bandwidth among VDCs in a fair way. This paper presents a bandwidth that guarantees enforcement framework NXT-Freedom, and this framework allocates the network resources on the basis of per-VDC fairness, which can achieve work conservation. In order to guarantee per-VDC fair allocation, a hierarchical max–min fairness algorithm is put forward in this paper. In order to ensure that the framework can be applied to non-congestion-free network core and achieve scalability, NXT-Freedom decouples the computation of per-VDC allocation from the execution of allocation, but it brings some CPU overheads resulting from bandwidth enforcement. We observe that there is no need to enforce the non-blocking virtual network. Leveraging this observation, we distinguish the virtual network type of VDC to eliminate part of the CPU overheads. The evaluation results of a prototype prove that NXT-Freedom can achieve the isolation of per-VDC performance, which also shows fast adaption to flow variation in cloud datacenter.
Dynamics Modelling and Simulation for Deployment Characteristics of Mesh Reflector Antennas
Authors: Jiang Xin , Bai Zhengfeng
Published: November 2020

The dynamics of mesh reflectors are characterized by large deformation when antennas undergo a large motion in the deployment process. In this work, absolute node coordinate formulation (ANCF), with the merit of precise description of large deformation, is employed to consider the flexibility of the reflector net. From a practical perspective, the effect of the torsional spring is incorporated into the dynamic governing equations by an equivalent mechanical model. Deployment simulation of the mesh reflector antennas is performed by a numerical procedure. It can be found that with the action of a degenerative driven force caused by the friction effect, the deployed process of antennas exhibits a character of asynchronous deployment. Additionally, a significant increase in tension during the final period of deployment has an intense influence on smooth deployment.
Assessing Suitable Areas of Common Grapevine (Vitis vinifera L.) for Current and Future Climate Situations: The CDS Toolbox SDM
Authors: Hinojos Mendoza Guillermo , Gutierrez Ramos Cesar Arturo , Heredia Corral Dulce María , Soto Cruz Ricardo , Garbolino Emmanuel
Published: November 2020

Climate Data Science (CDS) Toolbox Species Distribution Model (SDM) aims identifying the suitable areas for species, community of species and landscape units. This model is based on the use of 23 variables available over the Internet, for which any assumptions are formulated about their relationships with the spatial distribution of species. The application of CDS Toolbox SDM on the assessment of the potential impact of two scenarios of climate change (Representative Concentration Pathways RCP4.5 and RCP6.0) on the suitability of grapevine crops in France shows a general decrease of the most suitable areas for grapevine crops between 41% and 83% towards 2070 according to the current location of the vineyard parcels. The results underline a potential shift of the suitable areas in northern part of the French territory. They also show a potential shift of the most suitable areas in altitude (60 m in average) for RCP6.0 scenario. Finally, the model shows that RCP4.5 scenario should be more drastic than RCP6.0 scenario by 2050 and 2070. In effect, the model underlines a significant potential decrease of cultivated crops in the areas of high probably of suitable areas, according to the baseline scenario. This decrease would be of 630,000 ha for 2070 RCP4.5 scenario and 330,000 ha for 2070 RCP6.0 scenario.
Skill of Mesoscale Models in Forecasting Springtime Macrophysical Cloud Properties at the Savannah River Site in the Southeastern US
Authors: Noble Stephen , Viner Brian , Buckley Robert , Chiswell Steven
Published: November 2020

Predicting boundary layer clouds is important for the accurate modeling of pollutant dispersion. Higher resolution mesoscale models would be expected to produce better forecasts of cloud properties that affect dispersion. Using ceilometer observations, we assess the skill of two operational mesoscale models (RAMS and WRF) to forecast cloud base altitude and cloud fraction at the Savannah River Site in the southeastern US during the springtime. Verifications were performed at small spatial and temporal scales necessary for dispersion modeling. Both models were unreliable with a 50% (RAMS) and a 46% (WRF) rate of predicting clouds observed by the ceilometer which led to low cloud fraction predictions. Results indicated that WRF better predicted daytime cloud bases from convection that occurred frequently later in the period and RAMS better predicted nighttime cloud bases. Using root mean squared error (RMSE) to score the forecast periods also highlighted this diurnal dichotomy, with WRF scores better during the day and RAMS scores better at night. Analysis of forecast errors revealed divergent model cloud base biases—WRF low and RAMS high. A hybrid solution which weighs more heavily the RAMS nighttime forecasts and WRF daytime forecasts will likely provide the best prediction of cloud properties for dispersion.
Applications of GNSS-RO to Numerical Weather Prediction and Tropical Cyclone Forecast
Authors: Bai Weihua , Deng Nan , Sun Yueqiang , Du Qifei , Xia Junming , Wang Xianyi , Meng Xiangguang , Zhao Danyang , Liu Congliang , Tan Guangyuan , Liu Ziyan , Liu Xiaoxu
Published: November 2020

The global navigation satellite system (GNSS) radio occultation (RO) technique is an atmospheric sounding technique that originated in the 1990s. The data provided by this approach are playing a consistently significant role in atmospheric research and related applications. This paper mainly summarizes the applications of RO to numerical weather prediction (NWP) generally and specifically for tropical cyclone (TC) forecast and outlines the prospects of the RO technique. With advantages such as high precision and accuracy, high vertical resolution, full-time and all-weather, and global coverage, RO data have made a remarkable contribution to NWP and TC forecasts. While accounting for only 7% of the total observations in European Centre for Medium-Range Weather Forecasts’ (ECMWF’s) assimilation system, RO has the fourth-largest impact on NWP. The greater the amount of RO data, the better the forecast of NWP. In cases of TC forecasts, assimilating RO data from heights below 6 km and from the upper troposphere and lower stratosphere (UTLS) region contributes to the forecasting accuracy of the track and intensity of TCs in different stages. A statistical analysis showed that assimilating RO data can help restore the critical characteristics of TCs, such as the location and intensity of the eye, eyewall, and rain bands. Moreover, a non-local excess phase assimilation operator can be employed to optimize the assimilation results. With denser RO profiles expected in the future, the accuracy of TC forecast can be further improved. Finally, future trends in RO are discussed, including advanced features, such as polarimetric RO, and RO strategies to increase the number of soundings, such as the use of a cube satellite constellation.
Effect of Mild Salinity Stress on the Growth, Fatty Acid and Carotenoid Compositions, and Biological Activities of the Thermal Freshwater Microalgae Scenedesmus sp.
Authors: Elloumi Wiem , Jebali Ahlem , Maalej Amina , Chamkha Mohamed , Sayadi Sami
Published: November 2020

Carotenoids have strong antioxidant activity as well as therapeutic value. Their production has been induced in algae under stressful culture conditions. However, the extreme culture conditions lead to the Programmed Cell Death (PCD) of algae, which affects their growth and productivity. This study was performed to evaluate the effect of salinity on the physiological and biochemical traits of Scenedesmus sp., thermal freshwater microalgae from Northern Tunisia. It was cultured under different NaCl concentrations ranging from 0 to 60 g/L. Results showed a good growth and high contents of total chlorophyll and carotenoids in Scenedesmus sp. cultured at 10 g/L of NaCl (salt-stressed 10 (Ss10)). The pigment composition of the Ss10 extract was acquired using HPLC–MS, and showed that the carotenoid fraction is particularly rich in xanthophylls. Moreover, the antioxidant (DPPH and FRAP) and enzymatic inhibition (tyrosinase and elastase) activities of the Ss10 extract were higher compared to those of the control culture. In addition, the cytotoxicity test on B16 cells showed that the Ss10 extract was non-toxic for all tested concentrations below 100 µg/mL. It also showed a rich unsaturated fatty acid (FA) composition. Therefore, these findings suggest that Scenedesmus sp. strain cultivated under mild stress salinity could be a source of biomolecules that have potential applications in the nutraceutical and cosmeceutical industries.
Ellagic Acid Prevents Dopamine Neuron Degeneration from Oxidative Stress and Neuroinflammation in MPTP Model of Parkinson’s Disease
Authors: Ardah Mustafa T. , Bharathan Greeshma , Kitada Tohru , Haque M. Emdadul
Published: November 2020

Parkinson’s disease (PD) is one of the most common neurodegenerative diseases and is characterized by progressive dopaminergic neurodegeneration in the substantia nigra pars compacta area. In the present study, treatment of EA for 1 week at a dose of 10 mg/kg body weight prior to MPTP (25 mg/kg body weight) was carried out. MPTP administration caused oxidative stress, as evidenced by decreased activities of superoxide dismutase and catalase, and the depletion of reduced glutathione with a concomitant rise in the lipid peroxidation product, malondialdehyde. It also significantly increased the pro-inflammatory cytokines and elevated the inflammatory mediators like cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) in the striatum. Immunohistochemical analysis revealed a loss of dopamine neurons in the SNc area and a decrease in dopamine transporter in the striatum following MPTP administration. However, treatment with EA prior to MPTP injection significantly rescued the dopaminergic neurons and dopamine transporter. EA treatment further restored antioxidant enzymes, prevented the depletion of glutathione and inhibited lipid peroxidation, in addition to the attenuation of pro-inflammatory cytokines. EA also reduced the levels of COX-2 and iNOS. The findings of the present study demonstrate that EA protects against MPTP-induced PD and the observed neuroprotective effects can be attributed to its potent antioxidant and anti-inflammatory properties.
The Presence of Human Herpesvirus 6 in the Brain in Health and Disease
Authors: Santpere Gabriel , Telford Marco , Andrés-Benito Pol , Navarro Arcadi , Ferrer Isidre
Published: November 2020

The human herpesvirus 6 (HHV‐6) ‐A and ‐B are two dsDNA beta‐herpesviruses infectingalmost the entire worldwide population. These viruses have been implicated in multipleneurological conditions in individuals of various ages and immunological status, includingencephalitis, epilepsy, and febrile seizures. HHV‐6s have also been suggested as playing a role inthe etiology of neurodegenerative diseases such as multiple sclerosis and Alzheimer’s disease. Theapparent robustness of these suggested associations is contingent on the accuracy of HHV‐6detection in the nervous system. The effort of more than three decades of researching HHV‐6 in thebrain has yielded numerous observations, albeit using variable technical approaches in terms oftissue preservation, detection techniques, sample sizes, brain regions, and comorbidities. In thisreview, we aimed to summarize current knowledge about the entry routes and direct presence ofHHV‐6 in the brain parenchyma at the level of DNA, RNA, proteins, and specific cell types, inhealthy subjects and in those with neurological conditions. We also discuss recent findings relatedto the presence of HHV‐6 in the brains of patients with Alzheimer’s disease in light of availableevidence.
Isoharringtonine Induces Apoptosis of Non-Small Cell Lung Cancer Cells in Tumorspheroids via the Intrinsic Pathway
Authors: Lee Ji Hae , Park So-Young , Hwang Wonbin , Sung Jee Young , Cho Myoung-Lae , Shim Jaegal , Kim Yong-Nyun , Yoon Kyungsil
Published: November 2020

Lung cancer is the major cause of cancer-associated death worldwide, and development of new therapeutic drugs is needed to improve treatment outcomes. Three-dimensional (3D) tumorspheroids offer many advantages over conventional two-dimensional cell cultures due to the similarities to in vivo tumors. We found that isoharringtonine, a natural product purified from Cephalotaxus koreana Nakai, significantly inhibited the growth of tumorspheroids with NCI-H460 cells in a dose-dependent manner and induced apoptotic cell death in our 3D cell culture system. On the other hand, A549 tumorspheroids displayed low sensitivity to isoharringtonine-induced apoptosis. Nuclear receptor subfamily 4 group A member 1 (NR4A1) is an orphan nuclear receptor known to regulate proliferation and apoptosis of cancer cells. We observed that knockdown of NR4A1 dramatically increased isoharringtonine-induced cancer cell death in A549 tumorspheroids by activating the intrinsic apoptosis pathway. Furthermore, treatment with combined isoharringtonine and iNR4A1 significantly inhibited multivulva formation in a Caenorhabditis elegans model and tumor development in a xenograft mouse model. Taken together, our data suggest that isoharringtonine is a potential natural product for treatment of non-small cell lung cancers, and inhibition of NR4A1 sensitizes cancer cells to anti-cancer treatment.
Interfacing DNA with Gold Nanoparticles for Heavy Metal Detection
Authors: He Zhiyu , Yin Huiling , Chang Chia-Chen , Wang Guoqing , Liang Xingguo
Published: November 2020

The contamination of heavy metals (e.g., Hg, Pb, Cd and As) poses great risks to the environment and human health. Rapid and simple detection of heavy metals of considerable toxicity in low concentration levels is an important task in biological and environmental analysis. Among the many convenient detection methods for heavy metals, DNA-inspired gold nanoparticles (DNA-AuNPs) have become a well-established approach, in which assembly/disassembly of AuNPs is used for colorimetric signaling of the recognition event between DNA and target heavy metals at the AuNP interface. This review focuses on the recent efforts of employing DNA to manipulate the interfacial properties of AuNPs, as well as the major advances in the colorimetric detection of heavy metals. Beginning with the introduction of the fundamental aspects of DNA and AuNPs, three main strategies of constructing DNA-AuNPs with DNA binding-responsive interface are discussed, namely, crosslinking, electrostatic interaction and base pair stacking. Then, recent achievements in colorimetric biosensing of heavy metals based on manipulation of the interface of DNA-AuNPs are surveyed and compared. Finally, perspectives on challenges and opportunities for future research in this field are provided.
Attentional Measures of Memory in Typically Developing and Hypoxic–Ischemic Injured Infants
Authors: Wagner Jennifer B. , Jabès Adeline , Norwood Agatha , Nelson Charles A.
Published: November 2020

Hypoxic–ischemic injury (HII) at birth has been found to relate to differences in development, including decreased memory performance. The current study assessed recognition memory in 6- and 12-month-old HII infants and typically developing (TD) infants using two eye-tracking paradigms well suited to explore explicit memory processes early in life: visual paired comparison (VPC) and relational memory (RM). During the VPC, infants were familiarized to a face and then tested for their novelty preference immediately and after a two-minute delay. At 6 months, neither HII nor TD showed a VPC novelty preference at immediate delay, but at 12 months, both groups did; after the two-minute delay, no group showed a novelty preference. During RM, infants were presented with blocks containing a learning phase with three different scene–face pairs, and a test phase with one of the three scenes and all three faces appearing simultaneously. When there was no interference from other scene–face pairs between learning and test, 6-month-old TD showed evidence of an early novelty preference, but when there was interference, they revealed an early familiarity preference. For 12-month-old TD, some evidence for a novelty preference during RM was seen regardless of interference. Although HII and TD showed similar recognition memory on the VPC, when looking at RM, HII infants showed subtle differences in their attention to the familiar and novel faces as compared to their TD peers, suggesting that there might be subtle differences in the underlying memory processing mechanisms between HII and TD. More work is needed to understand how these attentional patterns might be predictive of later memory outcomes.
Developing a Virtual Multicultural Intervention for University Students
Authors: Black Kristen , Li Manyu
Published: November 2020

The recent surge in acts of violence motivated by intergroup biases in the United States are of great concern. If allowed to progress, these conditions could create an unwelcoming atmosphere and could foster further division within the United States. Based on previous culture-related studies, multiculturalism is a possible solution to reducing intergroup biases, as it positively affects implicit and explicit cultural attitudes, perceptions, as well as behaviors. The current study developed a virtual multiculturalism intervention as a means to improve intergroup relations and combat cultural biases within the undergraduate student population. Specifically, 249 undergraduate students were randomly assigned to the intervention condition and the control condition. Results showed that participants in the intervention condition had a significantly higher improvement in multiculturalism scores, supporting the incorporation of a virtual multiculturalism intervention in higher education. This study calls for the implementation of a better framework of understanding of how changes in multicultural events are perceived and how this can be used to create a more empathetic population who are more comfortable and understanding with one another. This inexpensive and timesaving model holds the possibility of being used in the future to aid in overcoming cultural differences between student populations and varying ethnics groups alike.
Sensitivity of Papilloma Virus-Associated Cell Lines to Photodynamic Therapy with Curcumin-Loaded Liposomes
Authors: Ambreen Ghazala , Duse Lili , Tariq Imran , Ali Uzma , Ali Sajid , Pinnapireddy Shashank R. , Bette Michael , Bakowsky Udo , Mandic Robert
Published: November 2020

Photodynamic therapy (PDT) is a minimally invasive therapeutic approach used in the treatment of various medical conditions and cancerous diseases, involving light, a photosensitizing substance, and oxygen. Curcumin, a naturally occurring compound, carries antitumor activities and potentially could be exploited as a photosensitizer in PDT. Only little is known about liposomal-encapsulated curcumin that could help in increasing the efficacy, stability, and bioavailability of this compound. This study investigates the in vitro effects of curcumin-loaded liposomes in combination with PDT. Three papilloma virus-associated cell lines were treated with curcumin-loaded liposomes corresponding to a curcumin concentration of 0–100 µmol/L for 4 h followed by illumination at 457 nm (blue) for 45, 136, and 227 s at a fluence of 220.2 W/m2 (100 mA) corresponding to 1, 3 and 5 J·cm−2. After 24 h, the biological outcome of the treatment was assessed with the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide), SYTO9/PI (propidium iodide), Annexin V-FITC (fluorescein isothiocyanate)/PI, clonogenic survival, and scratch (wound closure) assays. Photoactivation of curcumin-loaded liposomes led to a significant reduction in colony formation and migratory abilities, as well as to an increase in tumor cell death. The results point to the combination of curcumin-loaded liposomes with PDT as a potentially useful tool for the treatment of papillomavirus-associated malignancies.
Geriatric Oncology: From Research to Clinical Practice
Authors: de Glas Nienke A.
Published: November 2020

The number of older adults with cancer is strongly increasing due to the ageing of Western societies [...]
Recent Scientific Developments in Metastatic Prostate Cancer
Authors: Heidegger Isabel
Published: November 2020

In recent years, the treatment landscape of advanced prostate cancer has radically changed [...]
Advances in Plasma Oncology toward Clinical Translation
Authors: Lin Abraham , Stapelmann Katharina , Bogaerts Annemie
Published: November 2020

This Special Issue on “Advances in Plasma Oncology Toward Clinical Translation” aims to bring together cutting-edge research papers within the field in the context of clinical translation and application [...]
Liquid Biopsy for Solid Ophthalmic Malignancies: An Updated Review and Perspectives
Authors: Martel Arnaud , Baillif Stephanie , Nahon-esteve Sacha , Gastaud Lauris , Bertolotto Corine , Roméo Barnabé , Mograbi Baharia , Lassalle Sandra , Hofman Paul
Published: November 2020

Tissue biopsy is considered the gold standard when establishing a diagnosis of cancer. However, tissue biopsies of intraocular ophthalmic malignancies are hard to collect and are thought to be associated with a non-negligible risk of extraocular dissemination. Recently, the liquid biopsy (LB) has emerged as a viable, non-invasive, repeatable, and promising way of obtaining a diagnosis, prognosis, and theragnosis of patients with solid tumors. LB refers to blood, as well as any human liquid. The natural history of uveal melanoma (UM) and retinoblastoma (RB) are radically opposed. On the one hand, UM is known to disseminate through the bloodstream, and is, therefore, more accessible to systemic venous liquid biopsy. On the other hand, RB rarely disseminates hematogenous, and is, therefore, more accessible to local liquid biopsy by performing an anterior chamber puncture. In this review, we summarize the current knowledge concerning LB in UM, RB, conjunctival tumors, and choroidal metastases. We also develop the current limitations encountered, as well as the perspectives.