bluuebunny/crossref_metadata_2025 huggingface dataset:
Human-Robotic Cooperative Space Exploration
Authors: Martin Anne-Sophie
Published: February 2021

Since the beginning of space exploration, outer space has fascinated, captivated and intrigued people’s mind. The launch of the first artificial satellite—Sputnik—in 1957 by the Soviet Union, and the first man on the Moon in 1969 represent two significant missions in the space exploration history. In 1972, Apollo 17 marked the last human program on the lunar surface. Nevertheless, several robotic spacecrafts traveled to the Moon such as the Soviet Luna 24 in 1976 or more recently China’s Chang’e 4 in 2019 which touched down on its far side, the first time for a space vehicle. The international space community is currently assessing a return to the Moon in 2024 and even beyond in the coming decades, toward the Red Planet, Mars. Robots and rovers, for instance, Curiosity, Philae, Rosetta or Perseverance, will continue to play a major role in space exploration by paving the way for future long-duration missions on celestial bodies. Landing humans on the Moon, Mars, or on other celestial bodies, needs robotics because there are significant challenges to overcome from technological and physiological perspectives. Therefore, the support of machines and artificial intelligence is essential for developing future deep space programs as well as to reach a sustainable space exploration. One can imagine future circumstances where robots and humans are collaborating together on the Moon’s surface or on celestial bodies to undertake scientific research, to extract and to analyze space resources for a possible in situ utilization, as well as to build sites for human habitation and work. Indeed, different situations can be considered: (a) a robot, located on a celestial body, operated by a human on Earth or aboard a space station; (b) the in situ operation of a robot by an astronaut; (c) the interaction between a robot in outer space, manipulated from Earth and an astronaut; (d) the interaction between a robot operated from space and an astronaut; (e) the interaction between a robot with an artificial intelligence component and an astronaut; (f) the interaction between two robots in the case of on-orbit servicing. The principles of free exploration and cooperation are two core concepts in the international space legal framework. Hence, it is necessary to analyse the provisions on the five United Nations space treaties in the context of “human-robotic” cooperation. In addition, the development of a Code of Conduct for space exploration, involving humans and robots, might be needed in order to clearly identify the missions using robotic systems (e.g., mission’s purpose, area of operations) and to foresee scenarios of responsibility and liability in case of damage. Lastly, a review of the dispute settlement mechanisms is particularly relevant as international claims related to human–robot activities will inevitably occur given the fact that their collaboration will increase as more missions are being planned on celestial bodies.
Narratives in International Studies Research
Authors: Sadriu Behar
Published: February 2021

Narrative research is a trending topic in international studies, with a growing body of literature adopting limited insights from narratology, sociolinguistics, and related fields to construct new insights into the workings of international relations. These studies are mainly concerned with questions about how narratives can be used to shape future policy courses, or how they impact the identity of agents and actors. The proliferation of studies using “narratives” in international studies research has been widespread since the 2000s, following a series of puzzles raised by scholars writing on language and discourse more broadly, ever since the late 1980s as part of the “linguistic turn” in the field. The adoption of narrative theory into international relations research presents a series of important questions about the methodological implications of taking narratives seriously. These include inquiries into the extent to which scholars see themselves as contributing to current social, political, and economic configurations of the world through their own work. Other questions motivated by this include: can international relations scholarship contribute to narrative theories of their own, or are they content in borrowing insights from other disciplines? How far should scholars engage in assessing what actors say, rather than what they do? Or is this distinction a false one to begin with? Are there more or less potent narratives, and why do some become prominent while others do not? What is the causal significance of narratives, and what is the best way to study them?
Maritime Archaeological Research in Sub-Saharan Africa
Authors: Werz Bruno E.J.S.
Published: February 2021

Maritime archaeology in sub-Saharan Africa is still in its early development stage. An extensive literature survey indicated that relatively few projects of this nature have been undertaken in these parts. Even fewer warrant the adjective “scientific,” based on the dearth of peer-reviewed academic publications that have appeared to date. Based on the survey, it seems that most research is undertaken in South Africa, Kenya, and Namibia, and the emphasis therefore lies with these countries.
Sunspots
Authors: Rempel M. , Borrero J.M.
Published: February 2021

Sunspots are the most prominent manifestations of magnetic fields on the visible surface of the Sun (photosphere). While historic records mention sunspot observations by eye more than two thousand years ago, the physical nature of sunspots has been unraveled only in the past century starting with the pioneering work of Hale and Evershed. Sunspots are compact magnetic-field concentrations with a field strength exceeding 3,000 G in their center, a horizontal extent of about 30 Mm and typical lifetimes on the order of weeks. Research during the past few decades has focused on characterizing their stunning fine structure that became evident in high-resolution observations. The central part of sunspots (umbra) appears, at visible wavelengths, dark due to strongly suppressed convection (about 20% of the brightness of unperturbed solar granulation); the surrounding penumbra with a brightness of more than 75% of solar granulation shows efficient convective energy transport, while at the same time the constraining effects of magnetic field are visible in the filamentary fine structure of this region. The developments of the past 100 years have led to a deep understanding of the physical structure of sunspots. Key developments were the parallel advance of instrumentation; the advance in the interpretation of polarized light, leading to reliable inversions of physical parameters in the solar atmosphere; and the advance of modeling capabilities enabling radiation magnetohydrodynamic (MHD) simulations of the solar photosphere on the scale of entire sunspots. These developments turned sunspots into a unique plasma laboratory for studying the interaction of strong magnetic field with convection. The combination of refined observation and data analysis techniques provide detailed physical constraints, while numerical modeling has advanced to a level where a direct comparison with remote sensing observations through forward modeling of synthetic observations is now feasible. While substantial progress has been made in understanding the sunspot fine structure, fundamental questions regarding the formation of sunspots and sunspot penumbrae are still not answered.
Philosophy of Quantum Mechanics: Dynamical Collapse Theories
Authors: Bassi Angelo
Published: February 2021

Quantum Mechanics is one of the most successful theories of nature. It accounts for all known properties of matter and light, and it does so with an unprecedented level of accuracy. On top of this, it generated many new technologies that now are part of daily life. In many ways, it can be said that we live in a quantum world. Yet, quantum theory is subject to an intense debate about its meaning as a theory of nature, which started from the very beginning and has never ended. The essence was captured by Schrödinger with the cat paradox: why do cats behave classically instead of being quantum like the one imagined by Schrödinger? Answering this question digs deep into the foundation of quantum mechanics.

A possible answer is Dynamical Collapse Theories. The fundamental assumption is that the Schrödinger equation, which is supposed to govern all quantum phenomena (at the non-relativistic level) is only approximately correct. It is an approximation of a nonlinear and stochastic dynamics, according to which the wave functions of microscopic objects can be in a superposition of different states because the nonlinear effects are negligible, while those of macroscopic objects are always very well localized in space because the nonlinear effects dominate for increasingly massive systems. Then, microscopic systems behave quantum mechanically, while macroscopic ones such as Schrödinger’s cat behave classically simply because the (newly postulated) laws of nature say so.

By changing the dynamics, collapse theories make predictions that are different from quantum-mechanical predictions. Then it becomes interesting to test the various collapse models that have been proposed. Experimental effort is increasing worldwide, so far limiting values of the theory’s parameters quantifying the collapse, since no collapse signal was detected, but possibly in the future finding such a signal and opening up a window beyond quantum theory.
Latinx Assimilation
Authors: Ramírez Catherine S.
Published: February 2021

Latinx is a gender-neutral, gender non-binary, gender non-conforming, and gender-inclusive label that refers to Latin American–origin groups in the United States. Since there are, by some counts, roughly thirty of these groups, Latinx, like Asian Pacific American, is a pan-ethnic label.

Assimilation generally refers to a sociocultural process of absorption, of becoming more alike, and of boundary crossing (e.g., from margin to mainstream). When assimilation happens, the mainstream or the host society absorbs the minority or the newcomer, or the minority or the newcomer comes to resemble the majority or the host. In some instances, the majority or host takes on some of the minority’s or newcomer’s traits.

Assimilation is widely seen as an outcome of immigration to the United States. However, before it was associated with immigration, assimilation was linked to efforts to “civilize” Native Americans and African Americans. Assimilation is sometimes used synonymously with acculturation, Americanization, incorporation, and integration. In the master narrative of immigration and assimilation, immigrants arrive and never look back. They change their names, learn English, acquire capital, and participate in mainstream institutions and culture. Within a couple of generations, their descendants blend in.

Above all, assimilation is connected to ideas about who belongs in the United States. A pillar of the US nation-making project, it is a tool for distinguishing outsiders from insiders. More than a process of absorption, becoming more alike, and boundary crossing, assimilation is a relation of power. In some instances, groups are assimilated not as homologous peers but as distinct, subordinate, and even excluded others. These groups are, paradoxically, outsiders on the inside.

Because Latinxs are a heterogeneous group and not all Latinxs are immigrants, there is no and has never been a single or homogeneous Latinx experience of assimilation. Some Latinxs assimilate in ways in which assimilation is generally understood: they move from margin to mainstream and blend in with the majority. Others are folded into a community made up of people from the same country of origin and have relatively little interaction with the dominant society. Others are assimilated as outsiders on the inside. Latinx assimilation is frequently studied in the context of language (specifically, English and Spanish), bilingualism, citizenship, naturalization, upward mobility, labor, entrepreneurship, education, conflicts and alliances between immigrants and US-born Latinxs, gender relations, and generational differences (especially between immigrant parents and their US-born children). In short, there are many ways Latinxs have or have not assimilated. Likewise, there are many ways to narrate the histories of Latinx assimilation. There is no single or definitive history of Latinx assimilation.
Buddhism and Globalization
Authors: Borup Jørn
Published: February 2021

“Global Buddhism” can be broadly understood as the transnational and transcultural network of circulating Buddhists and dynamic flows of Buddhist ideas and practices. It is characterized by ideals of universally applicable values and individually accessible experiences transgressing historical and cultural particularities. Global Buddhism is one kind of globalized religion, being itself a specific domain within the general context of globalization. Globalization encompasses transnational processes of interchanging values, services, and products, typically related to the modern, capitalist world. However, globalization has also been understood as a framework constituting cultural and religious dynamics and centripetal forces involving circulating ideas, practices, and institutions in an open and interacting world. A broader spatial and temporal perspective on globalization situates it in broader historical contexts, but typically linking it to an affinity with postmodernity and with a historical focus on the time since the breakdown of the communist world. Proto-global elements of religion can likewise be found throughout history, especially in axial religions and in contexts of accelerated circulation and hybridization. Throughout Buddhist history, such elements have been characteristic of the religion’s evolution and dissemination. Mission and trade along the Silk Route and in southeast Asia created proto-global ramifications just like the advent of Western colonialism co-created reform movements and networks of people with international scope and impact on “Buddhist modernity.” Global aspects are thus inherently part of much of Buddhist history, but a more restricted use of the concept would place global Buddhism in the aftermath of modernity, typically pronounced in urban centres and de-territorialized (online) social networks. Resistance and relativization are potentially always part of global transfigurations has also been influential in Buddhist contexts. One specific kind of “glocal” Buddhism, (yet) mainly restricted to a North American context, concentrates on reactions towards the transfigurations of globalization. What constitutes this kind of “post-global Buddhism” is twofold: an unveiling of universalized, global Buddhism as basically particularized “white” Buddhism, and an ideological shift beyond such disguised hegemony envisioning itself with new practices, values, identities, and communities based on (gender and) ethnic/racial differentiation.
The Revolta da Chibata: Conscription, Corporal Punishment, and State Control of Free Afro-Brazilians
Authors: Morgan Zachary R.
Published: February 2021

On November 22, 1910, Rio de Janeiro was convulsed by the four-day Revolta da Chibata (Revolt of the Lash). Approximately half of the predominantly Afro-Brazilian sailors stationed in the nation’s capital—likely fifteen hundred to two thousand men—seized four modern battleships, removed their officers, and besieged the city. They complained of mistreatment, forced recruitment, low pay, and meager food, but their only demand in their first communication to the president was the cessation of corporal punishment in the Brazilian navy. Three of the four ships seized had been recently obtained by the Brazilian government from British shipyards; two were the first all-big-gun dreadnought-class battleships ever sold by the British to any foreign navy. Their 12-inch guns could near-simultaneously launch twelve 850-pound explosive shells at targets miles away, meaning that should they fire almost every part of the Brazilian capital city was under threat.

Their second communique to the president demanded an end to the “slavery as practiced in the Brazilian navy.” The institution’s nearly century-long traditions of forced conscription, systematic and ritualized lashing, long-term forced labor, and the conspicuous malnourishment of Afro-Brazilian men tempts comparison to the exploitation of the enslaved in preabolition Brazil, but other than a brief policy of purchase and subsequent freeing of enslaved men to serve in the armed forces during the Paraguayan War (1864–1870), naval service did not draw on the exploitation of the enslaved. Instead, it conscripted Brazil’s free Afro-descendant population; citizens who represented a 47 percent plurality of Brazil’s population, larger than either the free white or enslaved Black populations at the time of Brazil’s first national census in 1872.

The Brazilian navy was just one part in a series of institutions and legislative controls created and used to control Brazil’s free Afro-Brazilian population both before and after abolition in 1888. The freedom and citizenship of free Black men, women, and children was often ephemeral and regulated. Although Brazil lacked institutionalized racial segregation such as apartheid or Jim Crow, controls such as restriction on land ownership, police policies, military conscription, the manipulation of orphans, forced apprenticeship, and incarceration were implemented in such racialized ways that the overall outcome for Afro-Brazilians was similar. The navy’s acquisition of cutting-edge weapons of war created an opportunity for powerless Afro-descendant men to challenge the generally unacknowledged state systems of racial oppression and hierarchy.
Economics of Renewable Energy: A Comparison of Electricity Production Costs Across Technologies
Authors: Timilsina Govinda R. , Shah Kalim U.
Published: February 2021

The levelized costs of electricity generation for renewable energy technologies differ and fluctuate depending on factors including capital costs, operation and maintenance costs, utilization factors, and economic lives. In addition to these factors, In the case of fossil fuels, prices and heat rate are also responsible for fluctuations. There is a global movement in favor renewable energy. Many countries have announced carbon-free electricity within the next 30–40 years, which implies massive expansion of renewable energy technologies. The newer investment trends in electricity generation technologies indicate the same. Technological breakthroughs and cost reductions of energy storage technologies would further favor renewable energy technologies and would decrease their intermittency hurdles. Developments that expand the scaling effect of renewable energy and the potential improvement in efficiency through continued research and development could bring the cost of renewable energy further down in the future. When the levelized costs of electricity generation are estimated, the declining trends of renewable energy costs are observed and can to a large extent (but not fully) be explained by certain potential drivers. Particularly for wind and solar, these drivers include technological innovation/improvements that have increased efficiency, policy supports such as research and development funding, economy of scale both in the manufacturing of equipment (solar panels, wind turbines) and installation of plants, and monopoly rent dissipation due to increased number of manufacturers and suppliers. Competition among equipment manufacturers and project developers may also contribute to cost decline as could cost reduction through improved product efficiency related to technological improvements and innovations.
The Interpretation of Sequence Variants in Myeloid Neoplasms
Authors: Hanbazazh Mehenaz , Harada Shuko , Reddy Vishnu , Mackinnon Alexander Craig , Harbi Djamel , Morlote Diana
Published: February 2021

Objectives

To provide an overview of the challenges encountered during the interpretation of sequence variants detected by next-generation sequencing (NGS) in myeloid neoplasms, as well as the limitations of the technology with the goal of preventing the over- or undercalling of alterations that may have a significant effect on patient management.

Methods

Review of the peer-reviewed literature on the interpretation, reporting, and technical challenges of NGS assays for myeloid neoplasms.

Results

NGS has been integrated widely and rapidly into the standard evaluating of myeloid neoplasms. Review of the literature reveals that myeloid sequence variants are challenging to detect and interpret. Large insertions and guanine-cytosine-heavy areas prove technically challenging while frameshift and truncating alterations may be classified as variants of uncertain significance by tertiary analysis informatics pipelines due to their absence in the literature and databases.

Conclusions

The analysis and interpretation of NGS results in myeloid neoplasia are challenging due to the varied number of detectable gene alterations. Familiarity with the genomic landscape of myeloid malignancies and knowledge of the tools available for the interpretation of sequence variants are essential to facilitate translation into clinical and therapy decisions.
Lessons learned from pharmacy learner and educator experiences during early stages of COVID-19 pandemic
Authors: Sasser Caroline Welles , Wolcott Michael D , Morbitzer Kathryn A , Eckel Stephen F
Published: February 2021

Purpose

To explore pharmacy learner (eg, resident, fellow) and educator (eg, residency program director, preceptor) experiences and lessons learned during the coronavirus disease 2019 (COVID-19) pandemic.

Methods

In May 2020, attendees of the virtual Research in Education and Practice Symposium (REPS) were invited to participate in two 1-hour networking sessions. During these sessions, participants completed individual and group reflection exercises where they were asked questions about their experiences during the initial stages of the COVID-19 pandemic. Participants who volunteered submitted their responses through an electronic survey application. Anonymous responses were coded using thematic analysis to address the research questions.

Results

Sixty-eight and 38 participants, respectively, attended the 2 networking discussions. Participant-identified professional impacts of the COVID-19 crisis included unexpected learning experiences, greater adaptability, workflow and learning interruptions, and decreased productivity. Personal impacts included a greater focus on well-being, physical and social isolation, and changes in travel plans. Participants noted positive and negative emotions including acceptance, encouragement, anxiety, stress, and frustration. The main lessons learned focused on adaptability, gratitude, and empathy. Participants shared that they wished they would have known the anticipated duration of the pandemic, associated workflow changes, and reliance on technology prior to the start of the pandemic. In addition, they predicted that pharmacy practice will require changes to workflow flexibility, training expectations, the pharmacist’s role, and organizational structure.

Conclusion

The COVID-19 pandemic has positively and negatively impacted the professional and personal lives of pharmacy learners and educators, with the most notable impacts being in the areas of well-being and adaptability. Future research should explore the experiences of other workforce personnel and evaluate the long-term impact on pharmacy practice, patient outcomes, and workforce well-being and resiliency.
Improvements in Submental Contour up to 3 Years After ATX-101: Efficacy and Safety Follow-Up of the Phase 3 REFINE Trials
Authors: Humphrey Shannon , Cohen Joel L , Bhatia Ashish C , Green Lawrence J , Green Jeremy B , Bowen Beta
Published: February 2021

Background

ATX-101 (deoxycholic acid) significantly reduced submental fat (SMF) severity in two 24-week Phase 3 studies (REFINE-1 and REFINE-2).

Objectives

The aim of this study was to evaluate the durability of effect and long-term safety of ATX-101.

Methods

REFINE study patients who maintained ≥1-grade improvement on the Clinician-Reported SMF Rating Scale (CR-1 responders) 12 weeks after their last REFINE treatment were eligible for enrollment in this multicenter, double-blind, nontreatment, long-term, follow-up study (NCT02163902). The primary endpoint was CR-1 response at Years 1, 2, and 3. Patient-reported satisfaction, psychological impact, and adverse events were monitored.

Results

In total, 224 patients (ATX-101, n = 113; placebo, n = 111) were enrolled. Maintenance of CR-1 response was significantly better in the ATX-101 group than in the placebo group at Year 1 (86.4% vs 56.8%; P < 0.001), Year 2 (90.6% vs 73.8%; P = 0.014), and Year 3 (82.4% vs 65.0%; P = 0.03). Most (74%) ATX-101‒treated patients satisfied at 12 weeks remained satisfied at Year 3. Significant reductions from baseline in psychological impact scores were sustained through Year 3 (P < 0.001). No new treatment-related adverse events were reported.

Conclusions

Improvements in submental contour achieved with ATX-101 are maintained for 3 years in most patients. No new safety signals emerged.

Level of Evidence: 2
Management of Asymptomatic Patients With Textured Breast Implants: A Survey Analysis of Members of The Aesthetic Society
Authors: Frojo Gianfranco , Nguyen David , Boyd Louisa C , Roider Laura , Qureshi Hassan , Christopher Kara , Xu Kyle , Lund Herluf
Published: February 2021

Background

The management of breast implant–associated anaplastic large cell lymphoma (BIA-ALCL) has been well established. However, there is minimal data and significant variability in the management of asymptomatic textured implant (ATI) patients.

Objectives

The purpose of this study was to survey members of The Aesthetic Society on management of ATI patients in order to understand trends in practice patterns.

Methods

Members of The Aesthetic Society were queried via an electronic survey regarding management of ATI patients. Anonymous responses were collected by QualtricsXM (SAP, Walldorf, Germany) survey software. Descriptive statistics were used to examine the data, reported as frequencies. All analyses were performed with IBM SPSS Statistics 26 (IBM, Armonk, NY).

Results

The survey was sent to 2149 active and candidate members of The Aesthetic Society with a total of 470 responses (21.87% response rate). For ATI patients presenting with concerns regarding BIA-ALCL risk, 88.8% of respondents educate the patient with clinical follow-up and/or imaging. The other 11.2% of surgeons recommend implant removal with variability in capsulectomy technique. For ATI patients requesting surgery, 42.5% of respondents educate the patient with clinical follow-up and/or imaging. The majority of surgeons (57.6%) recommend removal of the implant, with 30% recommending total capsulectomies.

Conclusions

There is considerable variability in how members of The Aesthetic Society manage ATI patients who request surgery. Future regulatory agency and professional society collaborative efforts should continue to aim for generation of high-level scientific data to aid in the counseling and management of ATI patients.

Level of Evidence: 4
Transcriptome Regulation by Oncogenic ALK Pathway in Mammalian Cortical Development Revealed by Single-Cell RNA Sequencing
Authors: Mao Rui , Zhang Xiaoyun , Kong Youyong , Wu Shanshan , Huo Hai-qin , Kong Yue , Wang Zhen , Liu Yan , Jia Zhengping , Zhou Zikai
Published: February 2021

Precise regulation of embryonic neurodevelopment is crucial for proper structural organization and functioning of the adult brain. The key molecular machinery orchestrating this process remains unclear. Anaplastic lymphoma kinase (ALK) is an oncogenic receptor-type protein tyrosine kinase that is specifically and transiently expressed in developing nervous system. However, its role in the mammalian brain development is unknown. We found that transient embryonic ALK inactivation caused long-lasting abnormalities in the adult mouse brain, including impaired neuronal connectivity and cognition, along with delayed neuronal migration and decreased neuronal proliferation during neurodevelopment. scRNA-seq on human cerebral organoids revealed a delayed transition of cell-type composition. Molecular characterization identified a group of differentially expressed genes (DEGs) that were temporally regulated by ALK at distinct developmental stages. In addition to oncogenes, many DEGs found by scRNA-seq are associated with neurological or neuropsychiatric disorders. Our study demonstrates a pivotal role of oncogenic ALK pathway in neurodevelopment and characterized cell-type-specific transcriptome regulated by ALK for better understanding mammalian cortical development.
The vasculature: a therapeutic target in heart failure?
Authors: Luxán Guillermo , Dimmeler Stefanie
Published: February 2021

It is well established that the vasculature plays a crucial role in maintaining oxygen and nutrients supply to the heart. Increasing evidence further suggests that the microcirculation has additional roles in supporting a healthy microenvironment. Heart failure is well known to be associated with changes and functional impairment of the microvasculature. The specific ablation of protective signals in endothelial cells in experimental models is sufficient to induce heart failure. Therefore, restoring a healthy endothelium and microcirculation may be a valuable therapeutic strategy to treat heart failure. This review article will summarize the current understanding of the vascular contribution to heart failure with reduced or preserved ejection fraction. Novel therapeutic approaches including next generation pro-angiogenic therapies and non-coding RNA therapeutics, as well as the targeting of metabolites or metabolic signalling, vascular inflammation and senescence will be discussed.
Cardiac paraganglioma with sulfur subunit B gene mutation: a case report
Authors: Wu Chao , Yang Xu , Zhang Huimin , Song Yunhu
Published: February 2021

Background

Pheochromocytoma and paraganglioma is a rare disease with a prevalence of 0.2–0.6% in hypertensive patients from outpatient.

Case summary

A 21-year-old man complained of blood pressure elevation over one year and persistent hyperhidrosis near 5 years. In local hospital, a mass in the pericardial space with abundant blood flow was observed via echocardiography and confirmed under minimally invasive thoracotomy. With suspicion of malignant cardiac mass, the patient was recommended to transfer for further diagnosis and treatment. Combining evaluation for blood and urinary catecholamine levels, somatostatin receptor imaging, and iodine-131 metaiodobenzylguanidine scintigraphy, he was confirmed with the diagnosis of cardiac paraganglioma with blood supply from the right coronary artery identified via angiography. The cardiac tumour was then surgically resected and confirmed with a pathological diagnosis of paraganglioma. Subsequent genetic test suggested succinate dehydrogenase complex iron sulfur subunit B (SDHB) gene mutation. At 5-month follow-up, the patient was recovered with normal levels of blood catecholamines and catecholamine metabolites.

Discussion

Cardiac paraganglioma should be considered and evaluated in hypertensive patients with cardiac mass, even in non-typical population. Given a potential risk of developing malignancies, close follow-up is significant in patients with SDHB gene mutations.
Mid-term outcome of surgical treatment in patients with aorto-left ventricular tunnel
Authors: Liang Yu , Wang Jingjin , Li Dianyuan , Wan Linyuan , Gao Yiming , Liu Rui , Li Shoujun , Wang Hao , Pang Kunjing
Published: February 2021

OBJECTIVES

The purpose of this study was to review the outcomes of surgical treatment in patients with aorto-left ventricular tunnel and to investigate what kind of patient cohort is more likely to have adverse events.

METHODS

Twenty-one patients with a median age of 6.58 [interquartile range (IQR) 4.17–24.50] years who received surgical treatment of aorto-left ventricular tunnel from March 2002 to December 2019 were reviewed. The median follow-up time was 64.50 (IQR 25.15–120.50) months. Clinical characteristics, surgical methods and follow-up outcomes were summarized in separate groups of patients with or without preoperative aortic valve (AoV) issues. Composite adverse events were defined as death or requirement of reoperation. Time-related analysis of freedom from death and requirement of reoperation was performed with the Kaplan–Meier method.

RESULTS

The average tunnel size was 8.68 (standard deviation: 3.62) mm. The most common and the most important associated lesions were AoV lesions. Tunnels in 20 patients were closed with direct sutures or a patch. For 1 patient with an irreparable AoV, the tunnel was cut open simultaneously with aortic valve replacement and aortic root plasty. In the group of patients with preoperative AoV issues, 4 patients received aortic valve replacement with mechanical prosthetic valves and 6 patients received aortic valvuloplasty repair. The follow-up outcomes differed significantly between groups (the incidence of death was 15.38% and the incidence of requiring a reoperation was 46.15% in patients with preoperative AoV issues). In the group without preoperative AoV issues, there were no deaths and no reoperations (Fisher’s exact test; P = 0.018). The probability of freedom from death and of the requirement for reoperation between the 2 groups was not significantly different (log-rank, P = 0.09). Overall, the estimated probability of freedom from death and requirement of reoperation was 77.30% (standard error: 10.20%) [95% confidence interval (CI): 49.53–91.00] at 5 years, 67.64% (standard error: 12.70%) (95% CI: 36.71–85.84) at 10 years.

CONCLUSIONS

Patients with aorto-left ventricular tunnel with preoperative AoV issues are more prone to die or to require a reoperation. In contrast, patients without preoperative AoV issues can be free from death or reoperation for a longer period of time. Patients with preoperative AoV issues need much stricter postoperative long-term echocardiographic follow-up.
Transcriptome analysis ofCaenorhabditis eleganslacking heme peroxidase SKPO-1 reveals an altered response toEnterococcus faecalis
Authors: Liu Yi , Martinez-Martinez Daniel , Essmann Clara L , Cruz Melissa R , Cabreiro Filipe , Garsin Danielle A
Published: February 2021

The nematode Caenorhabditis elegans is commonly used as a model organism in studies of the host immune response. The worm encodes twelve peroxidase-cyclooxygenase superfamily members, making it an attractive model in which to study the functions of heme peroxidases. In previous work, loss of one of these peroxidases, SKPO-1 (ShkT-containing peroxidase), rendered C. elegans more sensitive to the human, Gram-positive pathogen Enterococcus faecalis. SKPO-1 was localized to the hypodermis of the animals where it also affected cuticle development as indicated by a morphological phenotype called “dumpy.” In this work, a better understanding of how loss of skpo-1 impacts both sensitivity to pathogen as well as cuticle development was sought by subjecting a deletion mutant of skpo-1 to transcriptome analysis using RNA sequencing following exposure to control (Escherichia coli) and pathogenic (E. faecalis) feeding conditions. Loss of skpo-1 caused a general upregulation of genes encoding collagens and other proteins related to cuticle development. On E. faecalis, these animals also failed to upregulate guanylyl cyclases that are often involved in environmental sensing. Hoechst straining revealed increased permeability of the cuticle and atomic force microscopy exposed the misalignment of the cuticular annuli and furrows. These findings provide a basis for better understanding of the morphological as well as the pathogen sensitivity phenotypes associated with loss of SKPO-1 function.
Convergence analysis of a numerical scheme for a tumour growth model
Authors: Droniou Jérôme , Nataraj Neela , Remesan Gopikrishnan C
Published: February 2021

A one–spatial dimensional tumour growth model Breward et al. ( 2001, 2002, 2003) that consists of three dependent variables of space and time is considered. These variables are volume fraction of tumour cells, velocity of tumour cells, and nutrient concentration. The model variables satisfy a coupled system of semilinear advection equation (hyperbolic), simplified linear Stokes equation (elliptic), and semilinear diffusion equation (parabolic) with appropriate conditions on the time–dependent boundary, which is governed by an ordinary differential equation. A reformulation of the model defined in a larger, fixed time–space domain is employed to overcome theoretical difficulties related to the time–dependent boundary. This reformulation reduces the complexity of the model by removing the need to explicitly track the time–dependent boundary. A numerical scheme that employs a finite volume method for the hyperbolic equation, a finite element method for the elliptic equation, and a backward Euler in time–mass lumped finite element in space method for the parabolic equation is developed. We establish the existence of a time interval $(0,T_{\ast })$ over which a convergent subsequence of the numerical approximations can be extracted using compactness techniques. The limit of any such convergent subsequence is proved to be a weak solution of the continuous model in an appropriate sense, which is called a threshold solution. Numerical tests and justifications that support the theoretical findings conclude the paper.
On Monge–Ampère Volumes of Direct Images
Authors: Finski Siarhei
Published: February 2021

This paper is devoted to the study of the asymptotics of Monge–Ampère volumes of direct images associated with high tensor powers of an ample line bundle. We study the leading term of this asymptotics and provide a classification of bundles saturating the topological bound of Demailly. In the special case of high symmetric powers of ample vector bundles, this provides a characterization of those admitting projectively flat Hermitian structures.
A platform for detecting cross-resistance in antibacterial drug discovery
Authors: Galarion Luiza H , Mohamad Merianne , Alzeyadi Zeyad , Randall Christopher P , O’Neill Alex J
Published: February 2021

Background

To address the growing antibiotic resistance problem, new antibacterial drugs must exert activity against pathogens resistant to agents already in use. With a view to providing a rapid means for deselecting antibacterial drug candidates that fail to meet this requirement, we report here the generation and application of a platform for detecting cross-resistance between established and novel antibacterial agents.

Methods

This first iteration of the cross-resistance platform (CRP) consists of 28 strains of defined resistance genotype, established in a uniform genetic background (the SH1000 strain of the clinically significant pathogen Staphylococcus aureus). Most CRP members were engineered through introduction of constitutively expressed resistance determinants on a low copy-number plasmid, with a smaller number selected as spontaneous resistant mutants.

Results

Members of the CRP collectively exhibit resistance to many of the major classes of antibacterial agent in use. We employed the CRP to test two antibiotics that have been proposed in the literature as potential drug candidates: γ-actinorhodin and batumin. No cross-resistance was detected for γ-actinorhodin, whilst a CRP member resistant to triclosan exhibited a 32-fold reduction in susceptibility to batumin. Thus, a resistance phenotype that already exists in clinical strains mediates profound resistance to batumin, implying that this compound is not a promising antibacterial drug candidate.

Conclusions

By detecting cross-resistance between established and novel antibacterial agents, the CRP offers the ability to deselect compounds whose activity is substantially impaired by existing resistance mechanisms. The CRP therefore represents a useful addition to the antibacterial drug discovery toolbox.
Assessing Risk of Breast Cancer: A Review of Risk Prediction Models
Authors: Kim Geunwon , Bahl Manisha
Published: February 2021

Accurate and individualized breast cancer risk assessment can be used to guide personalized screening and prevention recommendations. Existing risk prediction models use genetic and nongenetic risk factors to provide an estimate of a woman’s breast cancer risk and/or the likelihood that she has a BRCA1 or BRCA2 mutation. Each model is best suited for specific clinical scenarios and may have limited applicability in certain types of patients. For example, the Breast Cancer Risk Assessment Tool, which identifies women who would benefit from chemoprevention, is readily accessible and user-friendly but cannot be used in women under 35 years of age or those with prior breast cancer or lobular carcinoma in situ. Emerging research on deep learning-based artificial intelligence (AI) models suggests that mammographic images contain risk indicators that could be used to strengthen existing risk prediction models. This article reviews breast cancer risk factors, describes the appropriate use, strengths, and limitations of each risk prediction model, and discusses the emerging role of AI for risk assessment.
Detection ofRickettsia lusitaniaeAmongOrnithodoros sawaiiSoft Ticks Collected From Japanese Murrelet Seabird Nest Material From Gugul Island, Republic of Korea
Authors: Kim Heung-Chul , Jiang Ju , Hang Jun , Kim Su Yeon , Yun Seok-Min , Park Chang-uk , Kim Miran , Chong Sung-Tae , Farris Christina M , Richards Allen L , Klein Terry A
Published: February 2021

In a follow-up to the investigations of soft ticks identified from seabird nest soil and litter collected from coastal islands of the Republic of Korea (ROK), Ornithodoros sawaii and Ornithodoros capensis were assessed for the presence and identification of rickettsiae. Ticks collected from samples of 50–100 g of nest litter and soil from seabird nests were identified individually by morphological techniques, and species confirmed by sequencing of the mt-rrs gene. Subsequently, tick DNA preparations were screened for the presence of rickettsiae using a genus-specific nested PCR (nPCR) assay targeting the 17 kDa antigen gene. The amplicons from the 17 kDa assay and two additional nPCR assays targeting the gltA and ompB gene fragments were sequenced and used to identify the rickettsiae. A total of 134 soft ticks belonging to two species, O. sawaii Kitaoka & Suzuki 1973 (n = 125) and O. capensis Neumann 1901 (n = 9), were collected. Rickettsia lusitaniae DNA was detected and identified among O. sawaii ticks (n = 11, 8.8%) collected from nest litter and soil of the Japanese murrelet (Synthliboramphus wumizusume Temminck 1836) at Gugul Island along the western coastal area of the ROK. This study confirmed for the first time the presence of R. lusitaniae associated with O. sawaii collected from migratory seabird nests in the ROK.
A statistical measurement of the H i spin temperature in DLAs at cosmological distances
Authors: Allison James R
Published: February 2021

Evolution of the cosmic star formation rate (SFR) and molecular gas mass density is expected to be matched by a similarly strong evolution of the fraction of atomic hydrogen (H i) in the cold neutral medium (CNM). We use results from a recent commissioning survey for intervening 21-cm absorbers with the Australian Square Kilometre Array Pathfinder (ASKAP) to construct a Bayesian statistical model of the NH i-weighted harmonic mean spin temperature (Ts) at redshifts between z = 0.37 and 1.0. We find that Ts ≤ 274 K with 95 per cent probability, suggesting that at these redshifts the typical H i gas in galaxies at equivalent DLA column densities may be colder than the Milky Way interstellar medium (Ts, MW ∼ 300 K). This result is consistent with an evolving CNM fraction that mirrors the molecular gas towards the SFR peak at z ∼ 2. We expect that future surveys for H i 21-cm absorption with the current SKA pathfinder telescopes will provide constraints on the CNM fraction that are an order of magnitude greater than presented here.
Compact groups from semi-analytical models of galaxy formation – II. Different assembly channels
Authors: Díaz-Giménez E , Zandivarez A , Mamon G A
Published: February 2021

We study the formation of over 6000 compact groups (CGs) of galaxies identified in mock redshift-space galaxy catalogues built from semi-analytical models of galaxy formation (SAMs) run on the Millennium Simulations. We select CGs of four members in our mock SDSS galaxy catalogues and, for each CG, we trace back in time the real-space positions of the most massive progenitors of their four galaxies. By analysing the evolution of the distance of the galaxy members to the centre of mass of the group, we identify four channels of CG formation. The classification of these assembly channels is performed with an automatic recipe inferred from a preliminary visual inspection and based on the orbit of the galaxy with the fewest number of orbits. Most CGs show late assembly, with the last galaxy arriving on its first or second passage, while only 10–20 per cent form by the gradual contraction of their orbits by dynamical friction, and only a few per cent forming early with little subsequent contraction. However, a SAM from a higher resolution simulation leads to earlier assembly. Assembly histories of CGs also depend on cosmological parameters. At similar resolution, CGs assemble later in SAMs built on parent cosmological simulations of high density parameter. Several observed properties of mock CGs correlate with their assembly history: early-assembling CGs are smaller, with shorter crossing times, and greater magnitude gaps between their brightest two members, and their brightest galaxies have smaller spatial offsets and are more passive.
Parallel Genomic Changes Drive Repeated Evolution of Placentas in Live-Bearing Fish
Authors: van Kruistum Henri , Nijland Reindert , Reznick David N , Groenen Martien A M , Megens Hendrik-Jan , Pollux Bart J A
Published: February 2021

The evolutionary origin of complex organs challenges empirical study because most organs evolved hundreds of millions of years ago. The placenta of live-bearing fish in the family Poeciliidae represents a unique opportunity to study the evolutionary origin of complex organs, because in this family a placenta evolved at least nine times independently. It is currently unknown whether this repeated evolution is accompanied by similar, repeated, genomic changes in placental species. Here, we compare whole genomes of 26 poeciliid species representing six out of nine independent origins of placentation. Evolutionary rate analysis revealed that the evolution of the placenta coincides with convergent shifts in the evolutionary rate of 78 protein-coding genes, mainly observed in transporter- and vesicle-located genes. Furthermore, differences in sequence conservation showed that placental evolution coincided with similar changes in 76 noncoding regulatory elements, occurring primarily around genes that regulate development. The unexpected high occurrence of GATA simple repeats in the regulatory elements suggests an important function for GATA repeats in developmental gene regulation. The distinction in molecular evolution observed, with protein-coding parallel changes more often found in metabolic and structural pathways, compared with regulatory change more frequently found in developmental pathways, offers a compelling model for complex trait evolution in general: changing the regulation of otherwise highly conserved developmental genes may allow for the evolution of complex traits.