bluuebunny/crossref_metadata_2025 huggingface dataset:
Full-Observable Three-Phase State Estimation Algorithm Applied to Electric Distribution Grids
Authors: Soares Thiago Mota , Bezerra Ubiratan Holanda , Tostes Maria Emília de Lima
Published: April 2019

This paper proposes the development of a three-phase state estimation algorithm, which ensures complete observability for the electric network and a low investment cost for application in typical electric power distribution systems, which usually exhibit low levels of supervision facilities and measurement redundancy. Using the customers´ energy bills to calculate average demands, a three-phase load flow algorithm is run to generate pseudo-measurements of voltage magnitudes, active and reactive power injections, as well as current injections which are used to ensure the electrical network is full-observable, even with measurements available at only one point, the substation-feeder coupling point. The estimation process begins with a load flow solution for the customers´ average demand and uses an adjustment mechanism to track the real-time operating state to calculate the pseudo-measurements successively. Besides estimating the real-time operation state the proposed methodology also generates nontechnical losses estimation for each operation state. The effectiveness of the state estimation procedure is demonstrated by simulation results obtained for the IEEE 13-bus test network and for a real urban feeder.
Extraction Behavior of Different Conditioned S. Rubescens
Authors: Kröger Michael , Klemm Marco , Nelles Michael
Published: April 2019

Microalgae utilized for experiments are often not produced by the researchers that are doing experiments with them. The microalgae are made storable through thermal or freeze-drying by the producer. In an industrial scaled process, because of efficiency reasons, microalgae would not be dried, but processed directly. With that, the question is, if drying already could change the composition or structure that much, that a process scaled up from laboratory to productive scale with fresh microalgae would be less efficient or even would not work at all. The effect of freeze drying on the extraction behavior for the species Scenedesmus rubescens was investigated. It was obtained in freeze-dried condition and again was delivered in fresh state. The utilized microalgae were extracted with n-hexane, without and with different pretreatments (acidic hydrolysis and hydrothermal carbonization) to examine the differences in the yields. In conclusion, it was demonstrated that freeze drying harms the cell wall and therefore this process already influences the quantity of extracted lipids. Depending on the harshness of the treatment process for cell wall disruption this might influence the extracted yield when the algae are not freeze-dried. The quality of the extracted lipids does not change when freeze-dried.
Modeling and Forecasting Electric Vehicle Consumption Profiles
Authors: Gerossier Alexis , Girard Robin , Kariniotakis George
Published: April 2019

The growing number of electric vehicles (EV) is challenging the traditional distribution grid with a new set of consumption curves. We employ information from individual meters at charging stations that record the power drawn by an EV at high temporal resolution (i.e., every minute) to analyze and model charging habits. We identify five types of batteries that determine the power an EV draws from the grid and its maximal capacity. In parallel, we identify four main clusters of charging habits. Charging habit models are then used for forecasting at short and long horizons. We start by forecasting day-ahead consumption scenarios for a single EV. By summing scenarios for a fleet of EVs, we obtain probabilistic forecasts of the aggregated load, and observe that our bottom-up approach performs similarly to a machine-learning technique that directly forecasts the aggregated load. Secondly, we assess the expected impact of the additional EVs on the grid by 2030, assuming that future charging habits follow current behavior. Although the overall load logically increases, the shape of the load is marginally modified, showing that the current network seems fairly well-suited to this evolution.
Evaluation Method for the Hourly Average CO2eq. Intensity of the Electricity Mix and Its Application to the Demand Response of Residential Heating
Authors: Clauß John , Stinner Sebastian , Solli Christian , Lindberg Karen Byskov , Madsen Henrik , Georges Laurent
Published: April 2019

This work introduces a generic methodology to determine the hourly average CO2eq. intensity of the electricity mix of a bidding zone. The proposed method is based on the logic of input–output models and avails the balance between electricity generation and demand. The methodology also takes into account electricity trading between bidding zones and time-varying CO2eq. intensities of the electricity traded. The paper shows that it is essential to take into account electricity imports and their varying CO2eq. intensities for the evaluation of the CO2eq. intensity in Scandinavian bidding zones. Generally, the average CO2eq. intensity of the Norwegian electricity mix increases during times of electricity imports since the average CO2eq. intensity is normally low because electricity is mainly generated from hydropower. Among other applications, the CO2eq. intensity can be used as a penalty signal in predictive controls of building energy systems since ENTSO-E provides 72 h forecasts of electricity generation. Therefore, as a second contribution, the demand response potential for heating a single-family residential building based on the hourly average CO2eq. intensity of six Scandinavian bidding zones is investigated. Predictive rule-based controls are implemented into a building performance simulation tool (here IDA ICE) to study the influence that the daily fluctuations of the CO2eq. intensity signal have on the potential overall emission savings. The results show that control strategies based on the CO2eq. intensity can achieve emission reductions, if daily fluctuations of the CO2eq. intensity are large enough to compensate for the increased electricity use due to load shifting. Furthermore, the results reveal that price-based control strategies usually lead to increased overall emissions for the Scandinavian bidding zones as the operation is shifted to nighttime, when cheap carbon-intensive electricity is imported from the continental European power grid.
Energy-Efficient Thread Mapping for Heterogeneous Many-Core Systems via Dynamically Adjusting the Thread Count
Authors: Ju Tao , Zhang Yan , Zhang Xuejun , Du Xiaogang , Dong Xiaoshe
Published: April 2019

Improving computing performance and reducing energy consumption are a major concern in heterogeneous many-core systems. The thread count directly influences the computing performance and energy consumption for a multithread application running on a heterogeneous many-core system. For this work, we studied the interrelation between the thread count and the performance of applications to improve total energy efficiency. A prediction model of the optimum thread count, hereafter the thread count prediction model (TCPM), was designed by using regression analysis based on the program running behaviors and heterogeneous many-core architecture feature. Subsequently, a dynamic predictive thread mapping (DPTM) framework was proposed. DPTM uses the prediction model to estimate the optimum thread count and dynamically adjusts the number of active hardware threads according to the phase changes of the running program in order to achieve the optimal energy efficiency. Experimental results show that DPTM obtains a nearly 49% improvement in performance and a 59% reduction in energy consumption on average. Moreover, DPTM introduces about 2% additional overhead compared with traditional thread mapping for PARSEC (The Princeton Application Repository for Shared-Memory Computers) benchmark programs running on an Intel MIC (Many integrated core) heterogeneous many-core system.
Forest Canopy Can Efficiently Filter Trace Metals in Deposited Precipitation in a Subalpine Spruce Plantation
Authors: Tan Siyi , Zhao Hairong , Yang Wanqin , Tan Bo , Yue Kai , Zhang Yu , Wu Fuzhong , Ni Xiangyin
Published: April 2019

Trace metals can enter natural regions with low human disturbance through atmospheric circulation; however, little information is available regarding the filtering efficiency of trace metals by forest canopies. In this study, a representative subalpine spruce plantation was selected to investigate the net throughfall fluxes of eight trace metals (Fe, Mn, Cu, Zn, Al, Pb, Cd and Cr) under a closed canopy and gap-edge canopy from August 2015 to July 2016. Over the one-year observation, the annual fluxes of Al, Zn, Fe, Mn, Cu, Cd, Cr and Pb in the deposited precipitation were 7.29 kg·ha−1, 2.30 kg·ha−1, 7.02 kg·ha−1, 0.16 kg·ha−1, 0.19 kg·ha−1, 0.06 kg·ha−1, 0.56 kg·ha−1 and 0.24 kg·ha−1, respectively. The annual net throughfall fluxes of these trace metals were −1.73 kg·ha−1, −0.90 kg·ha−1, −1.68 kg·ha−1, 0.03 kg·ha−1, −0.03 kg·ha−1, −0.02 kg·ha−1, −0.09 kg·ha−1 and −0.08 kg·ha−1, respectively, under the gap-edge canopy and 1.59 kg·ha−1, −1.13 kg·ha−1, −1.65 kg·ha−1, 0.10 kg·ha−1, −0.04 kg·ha−1, −0.03 kg·ha−1, −0.26 kg·ha−1 and −0.15 kg·ha−1, respectively, under the closed canopy. The closed canopy displayed a greater filtering effect of the trace metals from precipitation than the gap-edge canopy in this subalpine forest. In the rainy season, the net filtering ratio of trace metals ranged from −66.01% to 89.05% for the closed canopy and from −52.32% to 33.09% for the gap-edge canopy. In contrast, the net filtering ratio of all trace metals exceeded 50.00% for the closed canopy in the snowy season. The results suggest that most of the trace metals moving through the forest canopy are filtered by canopy in the subalpine forest.
Dormancy-Breaking and Germination Requirements for Seeds of Sorbus alnifolia (Siebold & Zucc.) K.Koch (Rosaceae), a Mesic Forest Tree with High Ornamental Potential
Authors: Tang Yuhan , Zhang Keliang , Zhang Yin , Tao Jun
Published: April 2019

Sorbus alnifolia (Siebold & Zucc.) K.Koch (Rosaceae) is an economically important tree in the temperate forests of Eastern China. In recent decades, ever-increasing use and modification of forestlands have resulted in major degeneration of the natural habitat of S. alnifolia. Moreover, S. alnifolia seeds germinate in a complicated way, leading to a high cost of propagation. The current study aimed to determine the requirements for breaking seed dormancy and for germination as well as to characterize the type of seed dormancy present in this species. Moreover, the roles of temperature, cold/warm stratification, and gibberellic acid (GA3) in breaking dormancy were tested combined with a study of the soil seed bank. The results showed that intact seeds of S. alnifolia were dormant, requiring 150 days of cold stratification to achieve the maximum germination percentage at 5/15 °C. Exposure of the seeds to ranges of temperatures at 15/25 °C and 20/30 °C resulted in secondary dormancy. Scarifying seed coat and partial removal of the cotyledon promoted germination. Compared with long-term cold stratification, one month of warm stratification plus cold stratification was superior in breaking dormancy. Application of GA3 did not break the dormancy during two months of incubation. Seeds of S. alnifolia formed a transient seed bank. The viability of freshly matured S. alnifolia seeds was 87.65% ± 11.67%, but this declined to 38.25% after 6-months of storage at room temperature. Seeds of S. alnifolia have a deep physiological dormancy; cold stratification will be useful in propagating this species. The long chilling requirements of S. alnifolia seeds would avoid seedling death in winter.
How Often Do Protein Genes Navigate Valleys of Low Fitness?
Authors: Nelson Erik D. , Grishin Nick V.
Published: April 2019

To escape from local fitness peaks, a population must navigate across valleys of low fitness. How these transitions occur, and what role they play in adaptation, have been subjects of active interest in evolutionary genetics for almost a century. However, to our knowledge, this problem has never been addressed directly by considering the evolution of a gene, or group of genes, as a whole, including the complex effects of fitness interactions among multiple loci. Here, we use a precise model of protein fitness to compute the probability P ( s , Δ t ) that an allele, randomly sampled from a population at time t, has crossed a fitness valley of depth s during an interval t − Δ t , t in the immediate past. We study populations of model genes evolving under equilibrium conditions consistent with those in mammalian mitochondria. From this data, we estimate that genes encoding small protein motifs navigate fitness valleys of depth 2 N s ≳ 30 with probability P ≳ 0.1 on a time scale of human evolution, where N is the (mitochondrial) effective population size. The results are consistent with recent findings for Watson–Crick switching in mammalian mitochondrial tRNA molecules.
Earth Observation, Remote Sensing, and Geoscientific Ground Investigations for Archaeological and Heritage Research
Authors: Tapete Deodato
Published: April 2019

Building upon the positive outcomes and evidence of dissemination across the community of the first Special Issue “Remote Sensing and Geosciences for Archaeology”, the second edition of this Special Series of Geosciences dedicated to “Earth Observation, Remote Sensing and Geoscientific Ground Investigations for Archaeological and Heritage Research” collects a varied body of original scientific research contributions showcasing the technological, methodological, and interpretational advances that have been achieved in this field of archaeological and cultural heritage sciences over the last years. The fourteen papers, published after rigorous peer review, allowed the guest editor to make considerations on the capabilities, limitations, challenges, and perspectives of Earth observation (EO), remote sensing (RS), and geoscientific ground investigations with regard to: (1) archaeological prospection with high resolution satellite SAR and optical imagery; (2) high resolution documentation of archaeological features with drones; (3) archaeological mapping with LiDAR towards automation; (4) digital fieldwork using old and modern data; (5) field and archaeometric investigations to corroborate archaeological hypotheses; (6) new frontiers in archaeological research from space in contemporary Africa; and (7) education and capacity building in EO and RS for cultural heritage.
Association between Exposure to Ambient Air Pollution and Rheumatoid Arthritis in Adults
Authors: Shin Jiyoung , Lee Jiyoung , Lee Jueun , Ha Eun-Hee
Published: April 2019

Environmental factors may play roles in the development of rheumatoid arthritis (RA), and some studies have shown that air pollution was associated with the development of autoimmune disease. This study was designed to investigate the effect of air pollutants on the development of adult RA. A nested case-control cohort study was performed using the National Health Insurance Service-National Sample Cohort during 2002–2014 in Korea. Air pollution data were collected from the National Ambient Air Monitoring System (NAMIS), and exposure levels were extrapolated using geographic information systems. The group with RA (n = 444) was compared with a propensity score-matched control group (n = 1776), and one-year average concentrations of air pollution were predicted at each patient’s residence. The adjusted binary logistic regression analysis showed a positive association between O3 exposure and the incidence risk of RA for the third (odds ratios (OR) = 1.45, 95% confidence intervals (CI): 1.08–1.96) and fourth (OR = 1.35, 95% CI: 1.00–1.83) quartiles in adults over 20 years of age. The third quartile CO exposure was also associated with an increased risk of RA (OR = 1.57, 95% CI: 1.16–2.12). The results of this nationwide population-based study showed that a one-year exposure to CO and O3 in adults was associated with an increased risk of RA.
Utilization of a Mobile Dental Vehicle for Oral Healthcare in Rural Areas
Authors: Gao Sherry Shiqian , Yon Madeline Jun Yu , Chen Kitty Jieyi , Duangthip Duangporn , Lo Edward Chin Man , Chu Chun Hung
Published: April 2019

Oral diseases remain one of the major global public health challenges, and the worldwide urban–rural disparities in oral health are significant. Residents in rural areas generally suffer from a higher prevalence and severity of dental caries and periodontal disease, yet they face numerous difficulties and barriers in accessing oral healthcare. Conventional strategies, such as building of dental clinics or, hospitals, or the provision of outreach services by using disposable materials, are neither practical nor effective in rural settings. Mobile dental vehicles (MDVs) have been proposed as an alternative strategy to supplement the traditional oral healthcare in many regions. They have usually been utilized in school-based oral health programs, providing dental care to the homeless or migrants, and screening programs for the population for various oral diseases. Due to their high mobility, MDVs are particularly valuable for the underserved populations living in rural areas. The advance of dental devices enables MDVs to be operated in a self-sufficient manner. This allows the MDV to function almost as well as a conventional dental clinic, providing a variety of dental treatments, including scaling, restoration, and oral surgery. This article discusses the use of MDVs as a solution to urban–rural inequality in receiving oral healthcare.
The Bioaccumulation and Biodegradation of Testosterone by Chlorella vulgaris
Authors: Fu Mei , Deng Bixiang , Lü Hongjian , Yao Weizhi , Su Shengqi , Wang Dingyong
Published: April 2019

In the present study, the accumulation and degradation of testosterone by Chlorella vulgaris were studied. The results showed that C. vulgaris has a significant ability to eliminate testosterone by bioaccumulation and biodegradation, and during the 96 h experimental period, the data demonstrated that the accumulation of testosterone followed a sigmoidal accumulation pattern. At the end of the experiment, the bioconcentration percentages of testosterone by C. vulgaris in the high-concentration group and the low-concentration group were 11.49 ± 2.78% and 40.10 ± 1.98%, respectively, and the biodegradation percentages of testosterone were 69.64 ± 4.33% and 42.48 ± 1.92%, respectively. The rate of biodegradation of testosterone by C. vulgaris mainly depended on the relative initial concentration of testosterone. When the relative initial concentration of testosterone increases, the degradation may gradually change from zero-order kinetics to second-order kinetics.
Terrain Representation and Distinguishing Ability of Roughness Algorithms Based on DEM with Different Resolutions
Authors: Wu Jiang , Fang Junjie , Tian Jiangbo
Published: April 2019

Digital elevation model (DEM) resolution is closely related to the degree of expression of real terrain, the extraction of terrain parameters, and the uncertainty of statistical models. Therefore, based on DEMs with various resolutions, this paper explores the representation and distinguishing ability of different roughness algorithms to measure terrain parameters. Fuyang, a district of Hangzhou City with various landform types, was selected as the research area. Slope, root mean squared height, vector deviation, and two-dimensional continuous wavelet transform were selected as four typical roughness algorithms. The resolutions used were 5, 10, 25, and 50 m DEM on the scale for plains, hills, and mountainous areas. The statistical criteria of effect size and entropy were used as indicators to evaluate and analyze the different roughness algorithms. The results show that in terms of these measures: (1) The expression ability of the SLOPE and root mean squared height (RMSH) algorithms is better than that of the vector deviation method, while the two-dimensional continuous wavelet method based on frequency analysis emphasizes the terrain information within a certain range. (2) The terrain distinguishing ability of the SLOPE and RMSH is not sensitive to the changes in resolution, with the other two algorithms varying with the changes in resolution.
A 25-Intersection Model for Representing Topological Relations between Simple Spatial Objects in 3-D Space
Authors: Zhou Mengyun , Guan Qingfeng
Published: April 2019

With the rapid development of the economy, urgent needs for 3-D Geographical Information System (GIS) have sprung up in many application fields. The precise expression of three-dimensional topological relations is the foundation of spatial analysis, topological query, and spatial reasoning in three-dimensional space. In this paper, we subdivide the topological part “boundary” into face, edge, and vertex and propose a 25-intersection model (25IM) to represent topological relations between two simple spatial objects (point, line, region, and body) in 3-D space. An object in the 25IM has five topological parts: vertex, edge, face, interior, and exterior. The classification of topological relations is simplified by merging contain/inside and cover/coveredby. The 25IM describes ten groups of topological relations: body/body, body/region, body/line, body/point, region/region, region/line, region/point, line/line, line/point, and point/point. The 25IM is demonstrated to be more expressive than the 9IM and the DE-9IM, especially in distinguishing the detail situations when one object meets/covers another object (e.g., two bodies meet/cover at vertex, edge, or face).
Exogenous Delivery of Link N mRNA into Chondrocytes and MSCs—The Potential Role in Increasing Anabolic Response
Authors: Tendulkar Gauri , Ehnert Sabrina , Sreekumar Vrinda , Chen Tao , Kaps Hans-Peter , Golombek Sonia , Wendel Hans-Peter , Nüssler Andreas K. , Avci-Adali Meltem
Published: April 2019

Musculoskeletal disorders, such as osteoarthritis and intervertebral disc degeneration are causes of morbidity, which concomitantly burdens the health and social care systems worldwide, with massive costs. Link N peptide has recently been described as a novel anabolic stimulator for intervertebral disc repair. In this study, we analyzed the influence on anabolic response, by delivering synthetic Link N encoding mRNA into primary human chondrocytes and mesenchymal stromal cells (SCP1 cells). Furthermore, both cell types were seeded on knitted titanium scaffolds, and the influence of Link N peptide mRNA for possible tissue engineering applications was investigated. Synthetic modified Link N mRNA was efficiently delivered into both cell types and cell transfection resulted in an enhanced expression of aggrecan, Sox 9, and type II collagen with a decreased expression of type X collagen. Interestingly, despite increased expression of BMP2 and BMP7, BMP signaling was repressed and TGFβ signaling was boosted by Link N transfection in mesenchymal stromal cells, suggesting possible regulatory mechanisms. Thus, the exogenous delivery of Link N peptide mRNA into cells augmented an anabolic response and thereby increased extracellular matrix synthesis. Considering these findings, we suppose that the cultivation of cells on knitted titanium scaffolds and the exogenous delivery of Link N peptide mRNA into cells could mechanically support the stability of tissue-engineered constructs and improve the synthesis of extracellular matrix by seeded cells. This method can provide a potent strategy for articular cartilage and intervertebral disc regeneration.
Understanding Platelets in Infectious and Allergic Lung Diseases
Authors: Gomez-Casado Cristina , Villaseñor Alma , Rodriguez-Nogales Alba , Bueno Jose Luis , Barber Domingo , Escribese Maria M.
Published: April 2019

Emerging evidence suggests that platelets, cytoplasmic fragments derived from megakaryocytes, can no longer be considered just as mediators in hemostasis and coagulation processes, but as key modulators of immunity. Platelets have received increasing attention as the emergence of new methodologies has allowed the characterization of their components and functions in the immune continuum. Platelet activation in infectious and allergic lung diseases has been well documented and associated with bacterial infections reproduced in several animal models of pulmonary bacterial infections. Direct interactions between platelets and bacteria have been associated with increased pulmonary platelet accumulation, whereas bacterial-derived toxins have also been reported to modulate platelet function. Recently, platelets have been found extravascular in the lungs of patients with asthma, and in animal models of allergic lung inflammation. Their ability to interact with immune and endothelial cells and secrete immune mediators makes them one attractive target for biomarker identification that will help characterize their contribution to lung diseases. Here, we present an original review of the last advances in the platelet field with a focus on the contribution of platelets to respiratory infections and allergic-mediated diseases.
The Role of Histamine in the Pathophysiology of Asthma and the Clinical Efficacy of Antihistamines in Asthma Therapy
Authors: Yamauchi Kohei , Ogasawara Masahito
Published: April 2019

Mast cells play a critical role in the pathogenesis of allergic asthma. Histamine is a central mediator released from mast cells through allergic reactions. Histamine plays a role in airway obstruction via smooth muscle contraction, bronchial secretion, and airway mucosal edema. However, previous clinical trials of H1 receptor antagonists (H1RAs) as a treatment for asthma were not successful. In recent years, type 2 innate immunity has been demonstrated to be involved in allergic airway inflammation. Allergic asthma is defined by IgE antibody-mediated mast cell degranulation, while group 2 innate lymphoid cells (ILC2) induce eosinophilic inflammation in nonallergic asthma without allergen-specific IgE. Anti-IgE therapy has demonstrated prominent efficacy in the treatment of severe allergic asthmatics sensitized with specific perennial allergens. Furthermore, recent trials of specific cytokine antagonists indicated that these antagonists were effective in only some subtypes of asthma. Accordingly, H1RAs may show significant clinical efficacy for some subtypes of allergic asthma in which histamine is deeply associated with the pathophysiology.
Identification and Characterization of a Luteinizing Hormone Receptor (LHR) Homolog from the Chinese Mitten Crab Eriocheir sinensis
Authors: Yuan Li-Juan , Peng Chao , Liu Bi-Hai , Feng Jiang-Bin , Qiu Gao-Feng
Published: April 2019

Luteinizing hormone (LH), a pituitary gonadotropin, coupled with LH receptor (LHR) is essential for the regulation of the gonadal maturation in vertebrates. Although LH homolog has been detected by immunocytochemical analysis, and its possible role in ovarian maturation was revealed in decapod crustacean, so far there is no molecular evidence for the existence of LHR. In this study, we cloned a novel LHR homolog (named EsLHR) from the Chinese mitten crab Eriocheir sinensis. The complete sequence of the EsLHR cDNA was 2775bp, encoding a protein of 924 amino acids, sharing 71% amino acids identity with the ant Zootermopsis nevadensis LHR. EsLHR expression was found to be high in the ovary, while low in testis, gill, brain, and heart, and no expression in the thoracic ganglion, eye stalk, muscle, and hepatopancreas. Quantitative PCR revealed that the expression level of EsLHR mRNA was significantly higher in the ovaries in previtellogenic (Pvt), late vitellogenic (Lvt), and germinal vesicle breakdown (GVBD) stages than that in the vitellogenic (Mvt) and early vitellogenic (Evt) stages (P < 0.05), and, the highest and the lowest expression were in Lvt, and Evt, respectively. The strong signal was mainly localized in the ooplasm of Pvt oocyte as detected by in situ hybridization. The crab GnRH homolog can significantly induce the expression of EsLHR mRNA at 36 hours post injection in vivo (P < 0.01), suggesting that EsLHR may be involved in regulating ovarian development through GnRH signaling pathway in the mitten crab.
Cryo-EM Structure of a Begomovirus Geminate Particle
Authors: Xu Xiongbiao , Zhang Qing , Hong Jian , Li Zhenghe , Zhang Xiaokang , Zhou Xueping
Published: April 2019

Tobacco curly shoot virus, a monopartite begomovirus associated with betasatellite, causes serious leaf curl diseases on tomato and tobacco in China. Using single-particle cryo-electron microscopy, we determined the structure of tobacco curly shoot virus (TbCSV) particle at 3.57 Å resolution and confirmed the characteristic geminate architecture with single-strand DNA bound to each coat protein (CP). The CP–CP and DNA–CP interactions, arranged in a CP–DNA–CP pattern at the interface, were partially observed. This suggests the genomic DNA plays an important role in forming a stable interface during assembly of the geminate particle.
Multiplex Analysis Platform for Endocrine Disruption Prediction Using Zebrafish
Authors: Jarque Sergio , Ibarra Jone , Rubio-Brotons Maria , García-Fernández Jessica , Terriente Javier
Published: April 2019

Small fish are an excellent experimental model to screen endocrine-disrupting compounds, but current fish-based assays to detect endocrine disruption have not been standardized yet, meaning that there is not consensus on endpoints and biomarkers to be measured. Moreover, exposure conditions may vary depending on the species used as the experimental model and the endocrine pathway evaluated. At present, a battery of a wide range of assays is usually needed for the complete assessment of endocrine activities. With the aim of providing a simple, robust, and fast assay to assess endocrine-disrupting potencies for the three major endocrine axes, i.e., estrogens, androgens, and thyroid, we propose the use of a panel of eight gene expression biomarkers in zebrafish larvae. This includes brain aromatase (cyp19a1b) and vitellogenin 1 (vtg1) for estrogens, cytosolic sulfotransferase 2 family 2 (sult2st3) and cytochrome P450 2k22 (cyp2k22) for androgens, and thyroid peroxidase (tpo), transthyretin (ttr), thyroid receptor α (trα), and iodothyronine deiodinase 2 (dio2) for thyroid metabolism. All of them were selected according to their responses after exposure to the natural ligands 17β-estradiol, testosterone, and 3,3′,5-triiodo-L-thyronine (T3), respectively, and subsequently validated using compounds reported as endocrine disruptors in previous studies. Cross-talk effects were also evaluated for all compounds.
The Design and Application of Game Rewards in Youth Addiction Care
Authors: van Dooren Marierose M. M. , Visch Valentijn T. , Spijkerman Renske
Published: April 2019

Different types of rewards are applied in persuasive games to encourage play persistence of its users and facilitate the achievement of desired real-world goals, such as behavioral change. Persuasive games have successfully been applied in mental healthcare and may hold potential for different types of patients. However, we question to what extent game-based rewards are suitable in a persuasive game design for a substance dependence therapy context, as people with substance-related disorders show decreased sensitivity to natural rewards, which may result in different responses to commonly applied game rewards compared to people without substance use disorders. In a within-subject experiment with 20 substance dependent and 25 non-dependent participants, we examined whether play persistence and reward evaluation differed between the two groups. Results showed that in contrast to our expectations, substance dependent participants were more motivated by the types of rewards compared to non-substance dependent participants. Participants evaluated monetary rewards more positively than playing for virtual points or social rewards. We conclude this paper with design implications of game-based rewards in persuasive games for mental healthcare.
Deep Image Similarity Measurement Based on the Improved Triplet Network with Spatial Pyramid Pooling
Authors: Yuan Xinpan , Liu Qunfeng , Long Jun , Hu Lei , Wang Yulou
Published: April 2019

Image similarity measurement is a fundamental problem in the field of computer vision. It is widely used in image classification, object detection, image retrieval, and other fields, mostly through Siamese or triplet networks. These networks consist of two or three identical branches of convolutional neural network (CNN) and share their weights to obtain the high-level image feature representations so that similar images are mapped close to each other in the feature space, and dissimilar image pairs are mapped far from each other. Especially, the triplet network is known as the state-of-the-art method on image similarity measurement. However, the basic CNN can only handle fixed-size images. If we obtain a fixed size image via cutting or scaling, the information of the image will be lost and the recognition accuracy will be reduced. To solve the problem, this paper has proposed the triplet spatial pyramid pooling network (TSPP-Net) through combing the triplet convolution neural network with the spatial pyramid pooling. Additionally, we propose an improved triplet loss function, so that the network model can realize twice distance learning by only inputting three samples at one time. Through the theoretical analysis and experiments, it is proved that the TSPP-Net model and the improved triple loss function can improve the generalization ability and the accuracy of image similarity measurement algorithm.
Anesthetic Efficacy of Dexmedetomidine versus Midazolam When Combined with Remifentanil for Percutaneous Transluminal Angioplasty in Patients with Peripheral Artery Disease
Authors: Park Ji-Hyoung , Soh Sarah , Kwak Young-Lan , Kim Bosung , Choi Sohyun , Shim Jae-Kwang
Published: April 2019

Anesthesia for percutaneous transluminal angioplasty (PTA) involves a high-risk population having a broad spectrum of pain character and intensity. This study delved the anesthetic efficacy of dexmedetomidine versus midazolam, when used with remifentanil. Seventy patients scheduled for femoropopliteal PTA were randomized into two groups receiving either intermittent midazolam boluses (0.03–0.05 mg/kg) (MR group) or dexmedetomidine 0.2–0.7 μg/kg/h after a loading dose of 1.0 μg/kg for 10 min (DR group), both with remifentanil. The primary endpoint was the patients’ satisfaction (1–5, 5; extremely satisfied). Secondary endpoints included postprocedural pain scores (0–10, 10; worst imaginable pain) and adverse events. The satisfaction level of patients was significantly greater in the DR group compared with the MR group (4.0 [3.0, 5.0] versus 4.0 [2.0, 5.0] p = 0.021). The number of patients having a postprocedural pain score of at least 3 was significantly greater in the MR group compared with the DR group (10 [29%] versus 2 [6%], p = 0.013). The number of patients with hypotensive episodes was higher in the DR group (5 [14.7%] versus 0, p = 0.025), which could all be restored with ephedrine. The use of dexmedetomidine in conjunction with remifentanil may be a safe option that provides excellent patient satisfaction while potentially attenuating postprocedural pain.
The Influence of Maternal-Foetal Parameters on Concentrations of Zonulin and Calprotectin in the Blood and Stool of Healthy Newborns during the First Seven Days of Life. An Observational Prospective Cohort Study
Authors: Łoniewska Beata , Węgrzyn Dagmara , Adamek Karolina , Kaczmarczyk Mariusz , Skonieczna-Żydecka Karolina , Adler Grażyna , Jankowska Agata , Uzar Izabela , Kordek Agnieszka , Celewicz Marta , Łoniewski Igor
Published: April 2019

Background: It can be hypothetically assumed that maternal and perinatal factors influence the intestinal barrier. Methods: The study was conducted with 100 healthy, full-term newborns breastfed in the first week of life, with similar analyses for their mothers. Zonulin and calprotectin levels were used as intestinal permeability markers. Results: The median (range) zonulin concentrations (ng/mL) were in mothers: serum, 21.39 (6.39–57.54); stool, 82.23 (42.52–225.74); and newborns: serum cord blood, 11.14 (5.82–52.34); meconium, 54.15 (1.36–700.65); and stool at age seven days, 114.41 (29.38–593.72). Calprotectin median (range) concentrations (µg/mL) in mothers were: stool, 74.79 (3.89–211.77); and newborns: meconium, 154.76 (6.93–8884.11); and stool at age seven days 139.12 (11.89–627.35). The use of antibiotics during pregnancy resulted in higher zonulin concentrations in umbilical-cord serum and calprotectin concentrations in newborn stool at seven days, while antibiotic therapy during labour resulted in higher zonulin concentrations in the stool of newborns at seven days. Zonulin concentrations in the stool of newborns (at seven days) who were born via caesarean section were higher compared to with vaginal birth. With further analyses, caesarean section was found to have a greater effect on zonulin concentrations than prophylactic administration of antibiotics in the perinatal period. Pregnancy mass gain >18 kg was associated with higher calprotectin concentrations in maternal stool. Body Mass Index (BMI) increase >5.7 during pregnancy was associated with decreased zonulin concentrations in maternal stool and increased calprotectin concentrations in stool of mothers and newborns at seven days. There was also a negative correlation between higher BMI increase in pregnancy and maternal zonulin stool concentrations and a positive correlation between BMI increase in pregnancy and maternal calprotectin stool concentrations. Conclusion: Maternal-foetal factors such as caesarean section, antibiotic therapy during pregnancy, as well as change in mother’s BMI during pregnancy may increase intestinal permeability in newborns. Changes in body mass during pregnancy can also affect intestinal permeability in mothers. However, health consequences associated with increased intestinal permeability during the first days of life are unknown. Additionally, before the zonulin and calprotectin tests can be adopted as universal diagnostic applications to assess increased intestinal permeability, validation of these tests is necessary.
Delayed Initiation of Hemodialysis in Pregnant Women with Chronic Kidney Disease: Logistical Problems Impact Clinical Outcomes. An Experience from an Emerging Country
Authors: Hernández Rivera Juan Carlos H , Pérez López María Juana , Corzo Bermúdez Carlos Humberto , García Covarrubias Luis , Bermúdez Aceves Luis Antonio , Chucuan Castillo Conrado Alejandro , Salazar Mendoza Mariana , Piccoli Giorgina Barbara , Paniagua Sierra Ramón
Published: April 2019

Background: Chronic kidney disease (CKD) is associated with reduction of fertility and increased complications during pregnancy. The aim of this work is to analyze the clinical outcomes and risk factors in pregnant women who needed to start dialysis with different schedules in a public hospital in Mexico City, with particular attention on the interference of social and cultural elements as well as resource limitations. Material and methods: CKD women who needed dialysis in pregnancy over the period 2002–2014 and had with complete demographic and outcome data were included in this retrospective study. Clinical background, renal function during pregnancy, dialysis schedule, and clinical outcomes were reviewed. Results: Forty pregnancies in women with CKD who needed dialysis in pregnancy (39 singleton and one twin pregnancy) were studied: All patients were treated with hemodialysis. Thirty-nine patients had CKD stages 4 or 5 at referral; only one patient was of stage 3b. Dialysis was considered as indicated in the presence of fluid overload, unresponsive hypertension in the setting of advanced CKD, or when blood urea nitrogen values were increased to around 50 mg/dL. However, the initiation of dialysis was often delayed by days or weeks. The main reason for delaying the initiation of dialysis was patient (and family) refusal to start treatment. All patients were treated with thrice weekly dialysis, in 3–5 h sessions, with a target urea of <100 mg/dL. The number of hours on dialysis did not impact pregnancy outcomes. Ten pregnancies ended in miscarriages (8 spontaneous), 29 in pre-term delivery, and 1 in term delivery. Fifteen women were diagnosed with preeclampsia, one with eclampsia, and one with HELLP (hemolysis, elevated liver enzymes, low platelets,) syndrome. Twenty-four of the neonates survived (77.4% of live births); six singletons and one twin died as a consequence of prematurity. Two neonates displayed malformations: cleft palate with ear anomalies and duodenal atresia. Conclusions: CKD requiring hemodialysis in pregnancy is associated with a high frequency of complications; in the setting of delayed start and of thrice-weekly hemodialysis, dialysis schedules do not appear to influence outcomes.
Current Practice of Surgery for Benign Goitre—An Analysis of the Prospective DGAV StuDoQ|Thyroid Registry
Authors: Bartsch Detlef , Dotzenrath Cornelia , Vorländer Christian , Zielke Andreas , Weber Theresia , Buhr Heinz , Klinger Carsten , Lorenz Kerstin ,   the StuDoQ/Thyroid Study Group
Published: April 2019

Background: To evaluate the current indications, resection strategies and short-term outcomes of surgery for benign goitre in a country with endemic goitre. Methods: Data of patients who underwent surgery for benign goitre were retrieved from the prospective StuDoQ/Thyroid registry and retrospectively analysed regarding the patient’s demographics, indications for surgery, surgical procedures, histology, and perioperative outcomes. Results: In a 15-month period, 12,888 patients from 83 departments underwent thyroid resections for benign conditions. Main indications for surgery were exclusion of malignancy (68%), compression symptoms (20.7%) and hyperthyroidism (9.7%). Preoperative fine needle aspiration cytology was performed in only 12.2% of patients with the indication “exclusion of malignancy”. Thyroidectomy (49.8%) or hemithyroidectomy (36.9%) were performed in 86.7% of patients. Minimally invasive or alternative surgical techniques were applied in only 2.2%. Intraoperative neuromonitoring was used in 98.4% of procedures, in 97.5% of patients at least one parathyroid gland was visualized, and in 15.3% of patients parathyroid tissue was autografted, respectively. The rates of unilateral and bilateral transient recurrent nerve palsy were 3.6% and 0.07% of nerves at risk, the rate of transitory hypoparathyroidism was 15.3%. The rates of postoperative bleeding and wound infections requiring reoperation were 1.4% and 0.07%, respectively. Conclusions: The indication “exclusion of malignancy” is made too liberally, and there is a strong attitude to perform complete thyroid resections. Postoperative hypoparathyroidism is the major complication after surgery for benign thyroid disease, thus requiring more awareness.
Mesothelium and Malignant Mesothelioma
Authors: Hiriart Emilye , Deepe Raymond , Wessels Andy
Published: April 2019

The mesothelium is an epithelial structure derived from the embryonic mesoderm. It plays an important role in the development of a number of different organs, including the heart, lungs, and intestines. In this publication, we discuss aspects of the development of the mesothelium, where mesothelial structures can be found, and review molecular and cellular characteristics associated with the mesothelium. Furthermore, we discuss the involvement of the mesothelium in a number of disease conditions, in particular in the pathogenesis of mesotheliomas with an emphasis on malignant pleural mesothelioma (MPM)—a primary cancer developing in the pleural cavity.
Mineral Fertiliser Adoption and Land Productivity: Implications for Securing Stable Rice Production in Northern Ghana
Authors: Donkor Emmanuel , Owusu Victor
Published: April 2019

The promotion of farm innovations, such as mineral fertiliser, is one of the strategies for attaining the Sustainable Development Goals (SDGs) of zero hunger and poverty alleviation in developing countries. However, the adoption of mineral fertilisers has been low in Africa, particularly in Ghana. The present study not only analyses the impact of mineral fertiliser on the land productivity of rice farmers in northern Ghana but also determines factors that are associated with the adoption of mineral fertilisers using a primary dataset from 470 rice farmers. The study employs endogenous switching regression and propensity score matching approaches in the empirical analysis. The result shows that the adoption of mineral fertiliser tends to significantly increase the land productivity of rice farmers by improving soil fertility and making nutrients readily available to rice crops. The empirical finding further indicates that the adoption of mineral fertiliser is positively influenced by land area, seed, improved rice variety and row planting whereas farmers’ location and market distance exert negative effects on mineral fertiliser adoption. To maximise the land productivity of farmers, it is imperative for agricultural policy interventions to promote mineral fertiliser application by targeting key policy variables such as getting fertiliser input market outlets closer to farmers.
The Influence of Water/Cement Ratio and Air Entrainment on the Electric Resistivity of Ionically Conductive Mortar
Authors: Zhao Ruohong , Weng Yubin , Tuan Christopher Y. , Xu An
Published: April 2019

Ionically-conductive mortar can be used for indoor radiant heating partition walls. In these applications, mortar blocks are soaked in electrolyte solutions of CuSO4. The surfaces of the block are coated with sealant and epoxy resin afterwards to prevent evaporation. The mortar block becomes a heating element due to ionic conduction if a voltage is applied to the electrodes in the block. Its electrical conductivity depends on the dispersion of the electrolyte, and hence on the porosity of the mortar. The test specimens in this study were divided into four groups according to the different air entrainment agents, including aluminum powder and hydrogen peroxide as well as two air-entraining agents, SJ-2 and K12. Each group was manufactured with water/cement ratios in the range of 0.5 to 0.9. The test results showed that the conductivity of the mortar was strongly influenced by the air-entrainment and the water cement ratios. The volumetric electric resistivity and the associated microstructures of the mortar were investigated. The test results showed that the specimens made with aluminum powder and a water–cement ratio of 0.65–0.75 had high porosity. The porosity of those specimens was further increased by adding two different air-entraining agents. The specimens with aluminum powder and SJ-2, along with a water–cement ratio of 0.7 appeared to be the optimum mixture. Its resistivity was 19.37 Ω·m at 28 days under 25.31% porosity. The experimental results indicate that an ionically-conductive mortar can be produced by combining different air-entrainment agents with variable water-cement ratios to meet a specified electrical heating requirement.
Microencapsulation of Thymol in Poly(lactide-co-glycolide) (PLGA): Physical and Antibacterial Properties
Authors: Zhu Zhu , Min Tiantian , Zhang Xueji , Wen Yongqiang
Published: April 2019

Thymol has been shown to be a safe and effective broad-spectrum antimicrobial agent that can be used as a food preservative. However, its volatile characteristics and strong odor limit its use in food products. The microencapsulation of this essential oil in biopolymers could overcome these disadvantages. In this work, thymol-loaded poly(lactide-co-glycolide) (PLGA) microparticles were successfully prepared and the optimal encapsulation efficiency was obtained at 20% (w/w) thymol. Microparticles containing thymol presented a spherical shape and smooth surface. Microencapsulation significantly improved the thermal and storage stability of thymol. In vitro release profiles demonstrated an initial fast release followed by a slow and sustained release. Thymol-loaded microparticles had strong antibacterial activity against Escherichia coli and Staphylococcus aureus, and the effectiveness of their antibacterial properties was confirmed in a milk test. Therefore, the thymol-loaded microparticles show great potential for use as an antimicrobial and as preservation additives in food.