bluuebunny/crossref_metadata_2025 huggingface dataset:
Clinical Impact of Preoperative Magnetic Resonance Imaging in the Evaluation of Myometrial Infiltration and Lymph-Node Metastases in Stage I Endometrial Cancer
Authors: Bús Dorottya , Nagy Gyöngyi , Póka Róbert , Vajda György
Published: April 2021

Abstract: Purpose: In the developed world, endometrial cancer is one of the most common malignant gynecological cancer types. Due to the highly available diagnostic modalities and patient education, the early detection of the tumor leads to high overall survival.

Methods: In this study we analyzed the reliability of preoperative MRI findings in the staging of early stage endometrial cancer, as well as the clinical characteristics of patients underwent radical hysterectomy and the histopathologic evaluation of their tumor, with the retrospective data of radical hysterectomies performed in our hospital between 2010 and 2019.

Results: The accuracy, sensitivity, specificity, negative- and positive predictive value of MRI regarding stage were 94.7, 63.3, 94.8, 83.8, and 83.8%, respectively. The accuracy, sensitivity, specificity, negative- and positive predictive value of MRI for the detection of the myometrial invasion were 69.8, 80.0, 60.8, 64.3, and 77.5%, respectively. The accuracy, sensitivity, specificity, negative- and positive predictive value of MRI for the detection of lymph node metastases were 78.1, 28.6, 82, 11.1, and 93.6%, respectively.

Conclusions: Based on our results, MRI is the method of choice in terms of evaluating overall staging, as well as myometrial invasion, as its specificity and negative predictive value are relatively high. However, systematic lymphadenectomy showed improved cancer-related survival and recurrence-free survival. Our studies showed that the diagnosis of lymph node metastases is difficult with MRI modality since hyperplastic and metastatic nodes cannot easily differentiate, leading to a high percentage of false-positive results. Therefore, other imaging modalities may be used for more accurate evaluation. New findings of our study were that the role of the radiologist’s expertise in the evaluation of MR imaging plays an essential role in lowering false-negative and false-positive results. Therefore, findings evaluated by a radiologist with high-level expertise in gynecological imaging can complement the clinical findings and help substantially define the needed treatment.
The Use of Multispectral Imaging and Single Seed and Bulk Near-Infrared Spectroscopy to Characterize Seed Covering Structures: Methods and Applications in Seed Testing and Research
Authors: Mortensen Anders Krogh , Gislum René , Jørgensen Johannes Ravn , Boelt Birte
Published: April 2021

The objective of seed testing is to provide high-quality seeds in terms of high varietal identity and purity, germination capacity, and seed health. Across the seed industry, it is widely acknowledged that quality assessment needs an upgrade and improvement by inclusion of faster and more cost-effective techniques. Consequently, there is a need to develop and apply new techniques alongside the classical testing methods, to increase efficiency, reduce analysis time, and meet the needs of stakeholders in seed testing. Multispectral imaging (MSI) and near-infrared spectroscopy (NIRS) are both quick and non-destructive methods that attract attention in seed research and in the seed industry. This review addresses the potential benefits and challenges of using MSI and NIRS for seed testing with a comprehensive focus on applications in physical and physiological seed quality as well as seed health.
Evaluation of a Configurable, Mobile and Modular Floor-Pen System for Group-Housing of Laboratory Rabbits
Authors: Matzek Dana , Baldauf Hanna-Mari , Schieweck Rico , Popper Bastian
Published: April 2021

The major responsibility of researchers and laboratory animal facilities is to ensure animal well-being during the time of acclimatization, experiments, and recovery. In this context, animal housing conditions are of utmost importance. Here, we implemented a mobile and modular floor-pen housing system for laboratory rabbits that combines rabbits’ natural behavioral requirements and the high hygiene standards needed in biomedical science. Twelve female New Zealand White (NZW) rabbits were single- or group-housed for 12 months in mobile and modular floor-pens. Their general health status was evaluated at the end of the experimental setup. Further, we performed behavioral analysis of six additional NZW females group-housed for eight weeks in pens of two different sizes. We show that our improved housing concept supported species-specific behavioral patterns. Taken together, our housing system provides an optimal setup for rabbits in animal facilities that combines strict requirements for animal experiments with animal welfare.
Biochemistry and Immune Biomarkers Indicate Interacting Effects of Pre- and Postnatal Stressors in Pigs across Sexes
Authors: Rymut Haley E. , Rund Laurie A. , Bolt Courtni R. , Villamil María B. , Bender Diane E. , Southey Bruce R. , Johnson Rodney W. , Rodriguez-Zas Sandra L.
Published: April 2021

The effects of maternal immune activation (MIA) elicited by a prenatal stressor and postnatal metabolic or immune stressors on chemical and inflammatory biomarkers were studied in male and female pigs. Pigs exposed to MIA elicited by porcine reproductive and respiratory syndrome virus and matching controls were assigned at two months of age to fasting stress, immune stress, or a saline group. The serum levels of over 30 chemistry and immune analytes were studied. Significantly low levels of blood urea nitrogen were detected in females exposed to MIA, while the highest creatinine levels were identified in fasting females exposed to MIA. The levels of interferon gamma and interleukin 8 were highest in pigs exposed to postnatal immune challenge. The profiles suggest that MIA may sensitize pigs to postnatal stressors for some indicators while making them more tolerant of other stressors. Effectiveness of practices to ameliorate the impact of postnatal stressors on the physiology of the pig could be enhanced by considering the prenatal stress circumstances.
Emmer-Based Beverage Fortified with Fruit Juices
Authors: Dimitrellou Dimitra , Kandylis Panagiotis , Kokkinomagoulos Evangelos , Hatzikamari Magdalini , Bekatorou Argyro
Published: April 2021

Nowadays, there is a growing consumer demand for non-dairy functional foods due to several health issues related to milk and dairy consumption and increasing vegetarianism. Following that trend, in the present study emmer-based beverages were developed after flour gelatinization, fortification with fruit juices (blueberry, aronia, and grape) and fermentation with the potential probiotic strain Lactiplantibacillus plantarum 2035. The produced beverages were subjected to a 4-week storage at 4 °C. The addition of juices significantly affected the physicochemical characteristics of the beverages, while resulting in increased red color. Total phenolic content (22.3–31.9 mg gallic acid equivalents 100 g−1) and antioxidant activity (94–136 μmol Trolox equivalents 100 g−1) were significantly higher in the case of aronia juice followed by blueberry and grape juice. All beverages showed high values of apparent viscosity and water-holding capacity. Lactiplantibacillus plantarum 2035 retained high viable counts during storage especially in beverages with fruit juices (>108 cells g−1 up to 21st day) revealing a positive effect of the juices. The obtained results show that emmer-based beverages fortified with fruit juices (aronia, blueberry, and grape) have a great potential as carriers of probiotics, prebiotics and other functional compounds and may be served as an ideal alternative to dairy products.
Performance Evaluation Metrics for Multi-Objective Evolutionary Algorithms in Search-Based Software Engineering: Systematic Literature Review
Authors: Nuh Jamal Abdullahi , Koh Tieng Wei , Baharom Salmi , Osman Mohd Hafeez , Kew Si Na
Published: April 2021

Many recent studies have shown that various multi-objective evolutionary algorithms have been widely applied in the field of search-based software engineering (SBSE) for optimal solutions. Most of them either focused on solving newly re-formulated problems or on proposing new approaches, while a number of studies performed reviews and comparative studies on the performance of proposed algorithms. To evaluate such performance, it is necessary to consider a number of performance metrics that play important roles during the evaluation and comparison of investigated algorithms based on their best-simulated results. While there are hundreds of performance metrics in the literature that can quantify in performing such tasks, there is a lack of systematic review conducted to provide evidence of using these performance metrics, particularly in the software engineering problem domain. In this paper, we aimed to review and quantify the type of performance metrics, number of objectives, and applied areas in software engineering that reported in primary studies—this will eventually lead to inspiring the SBSE community to further explore such approaches in depth. To perform this task, a formal systematic review protocol was applied for planning, searching, and extracting the desired elements from the studies. After considering all the relevant inclusion and exclusion criteria for the searching process, 105 relevant articles were identified from the targeted online databases as scientific evidence to answer the eight research questions. The preliminary results show that remarkable studies were reported without considering performance metrics for the purpose of algorithm evaluation. Based on the 27 performance metrics that were identified, hypervolume, inverted generational distance, generational distance, and hypercube-based diversity metrics appear to be widely adopted in most of the studies in software requirements engineering, software design, software project management, software testing, and software verification. Additionally, there are increasing interest in the community in re-formulating many objective problems with more than three objectives, yet, currently are dominated in re-formulating two to three objectives.
CFD-Simulink Modeling of the Inflatable Solar Dryer for Drying Paddy Rice
Authors: Salvatierra-Rojas Ana , Ramaj Iris , Romuli Sebastian , Müller Joachim
Published: April 2021

Small-scale farmers in developing Asian countries have minimal agricultural mechanisms available to them. In the Philippines, postharvest losses in rice production can reach about 36% in the drying process alone. Thus, the inflatable solar dryer (ISD) was developed through the collaboration of the University of Hohenheim, the International Rice Research Institute, and GrainPro Philippines Inc. Although the ISD was successfully tested with different agricultural products, further characterization of the ISD design is required for predicting the drying performance. To this end, the airflow behavior in the ISD was simulated using computational fluid dynamics (CFD) via ANSYS Fluent. Moreover, a thermal model was developed in MATLAB/Simulink by taking into account heat transfer in the heating area and coupled heat and mass transfer within the drying area. Three batches of drying experiments were performed and airflow measurements were taken inside the dryer to validate the models. The MATLAB/Simulink model was further used to predict the drying performance under various weather conditions spanning 10 years. The simulated temperatures and moisture content in the ISD showed high accuracy (mean absolute percentage error (MAPE) < 10%) with the experimental data. The proposed dynamic model provides an efficient computational tool that can be applied to predict the drying performance and to optimize the ISD design.
Broadband Metallic Carbon Nanotube Saturable Absorber for Ultrashort Pulse Generation in the 1500–2100 nm Spectral Range
Authors: Pawliszewska Maria , Tomaszewska Dorota , Soboń Grzegorz , Dużyńska Anna , Zdrojek Mariusz , Sotor Jarosław
Published: April 2021

Herein, we report on the possibility of ultrashort laser pulse generation in the broadband spectral range using a saturable absorber based on free-standing metallic carbon nanotube thin film. Erbium, thulium, and holmium-doped all-fiber lasers were mode-locked with a single saturable absorber containing a 300 nm thick material layer. Subpicosecond pulses were generated at 1559, 1938, and 2082 nm. Our work validates the broadband operation of metallic carbon nanotube-based saturable absorbers and highlights the suitable performance of nanomatematerial for ultrafast photonic applications.
Analytical and Measured Effects of Short and Heavy Rail Cars on Railway Bridges in the USA
Authors: Rakoczy Anna M. , Otter Duane E. , Dick Stephen M.
Published: April 2021

The overall number of railcars recorded in the North American railcar fleet from 2010 to 2015 increased about 5%; the number of all 130 tonne (286,000 lb) gross weight railcars (heavy axle load (HAL) railcars) increased 19%. The increase in shipments in short railcars increases the loading on railway bridges, especially the 12.8-m railcars, commonly used to ship sand and cement, which is approximately a 25% increase in load per unit length compared to 16.2-m coal cars. Significant differences between maximum effects of shorter railcars and common 16.2-m railcars were predicted in analysis for bridge spans longer than 18.3 m. The differences were more prominent on spans 24.4 m and longer. This study presents analytical and measured effects of freight railcars on a two-span truss bridge, with spans of 61 m and 33.5 m, and a 35-m riveted steel deck plate girder (DPG) bridge. The investigation confirmed that short railcars cause higher load effects on main bridge components: the 35-m riveted steel DPG has 28% higher stresses at mid-span, while in the truss, the difference in stresses depends on the location of the member and ranges from 15 to 35%.
Quaternion Codes in MIMO System of Dual-Polarized Antennas
Authors: Ali Sajid , Qureshi Sara Shakil , Hassan Syed Ali
Published: April 2021

The use of quaternion orthogonal designs (QODs) to describe point-to-point communication among dual-polarized antennas has the potential to provide higher rate orthogonal and quasi-orthogonal complex designs exploiting polarization diversity among space and time diversities. Furthermore, it is essential to have a space time block code (STBC) which offers a linear and decoupled decoder which quasi-orthogonal designs fail to attain. In this paper, we show how the realm of quaternions unexpectedly offers us a possible solution and codes obtained from quaternion designs mostly achieve both linear and decoupled decoders. This motivated us to perform an indispensable search for QODs such that the code rate is bounded below by 1/2 and does not sharply decrease as the number of transmit antennas increases. It is shown that three famous recursive techniques do not satisfy this criteria and their code rates decrease rather rapidly. Therefore, we propose another method of constructing quaternion designs suitable for any number of transmit antennas and verify that these attain linear and decoupled decoders with the system model based on quaternionic channel. It is shown that such designs outperform others in terms of transmit diversity, code rates and the optimality of the proposed decoder is validated through simulation results.
Bacteria-Mediated Synthesis of Silver and Silver Chloride Nanoparticles and Their Antimicrobial Activity
Authors: Ghiuta Ioana , Croitoru Catalin , Kost Joseph , Wenkert Rodica , Munteanu Daniel
Published: April 2021

Within the frame of this work, the synthesis of silver nanoparticles (Ag NPs) and silver chloride nanoparticles (AgCl NPs) as mediated by microbes has been investigated. The nanoparticles were reduced from a silver nitrate precursor by the presence of bacteria, like Raoultella planticola and Pantoea agglomerans. The results show that the characteristic surface plasmon resonance absorption band occurs at about 440 nm. Nanoparticles were also characterized with the help of scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), transmission electron microscopy (TEM), and X-ray diffraction (XRD), which showed the formation of spherical Ag/AgCl NPs with a centered cubic crystal structure and a mean particle size of around 10–50 nm. Assays for antimicrobial activity of the biosynthesized nanoparticles demonstrated meaningful results against microorganisms such as Staphylococcus aureus, Streptococcus pyogenes, Salmonella, and Bacillus amyloliquefaciens. Furthermore, this study shows that the combination of the obtained nanoparticles with standard antibiotics may be useful in the fight against emerging microbial drug resistance.
A Novel Thermostable Keratinase from Deinococcus geothermalis with Potential Application in Feather Degradation
Authors: Tang Yin , Guo Leizhou , Zhao Mingming , Gui Yuan , Han Jiahui , Lu Wei , Dai Qilin , Jiang Shijie , Lin Min , Zhou Zhengfu , Wang Jin
Published: April 2021

Keratinase can specifically attack disulfide bridges in keratin to convert them from complex to simplified forms. Keratinase thermal stability has drawn attention to various biotechnological industries. In this study, a keratinase DgeKer was identified from a slightly thermophilic species, D. geothermalis. The in silico analysis showed that DgeKer is composed of signal peptide, N-terminal propeptide, mature domain, and C-terminal extension. DgeKer and its C-terminal extension-truncated enzyme (DgeKer-C) were cloned and expressed in E. coli. The purified DgeKer and DgeKer-C showed maximum activity at 70 °C and pH 9–The thermal stability assay (60 °C) showed that the half-life value of DgeKer and DgeKer-C were 103.45 min and 169.10 min, respectively. DgeKer and DgeKer-C were stable at the range of pH from 9 to 11 and showed good tolerance to some metal ions, surfactants and organic solvent. Furthermore, DgeKer could degrade feathers at 70 °C for 60 min. However, the medium became turbid with obvious softening of barbules after being treated with DgeKer-C, which might be due to C-terminal extension. In summary, a thermostable keratinase DgeKer with high efficiency degradation of feathers may have great potential in industry.
Maize and Sorghum as Raw Materials for Brewing, a Review
Authors: Dabija Adriana , Ciocan Marius Eduard , Chetrariu Ancuta , Codină Georgiana Gabriela
Published: April 2021

Brewing is among the oldest biotechnological processes, in which barley malt and—to a lesser extent—wheat malt are used as conventional raw materials. Worldwide, 85–90% of beer production is now produced with adjuvants, with wide variations on different continents. This review proposes the use of two other cereals as raw materials in the manufacture of beer, corn and sorghum, highlighting the advantages it recommends in this regard and the disadvantages, so that they are removed in technological practice. The use of these cereals as adjuvants in brewing has been known for a long time. Recently, research has intensified regarding the use of these cereals (including in the malted form) to obtain new assortments of beer from 100% corn malt or 100% sorghum malt. There is also great interest in obtaining gluten-free beer assortments, new nonalcoholic or low-alcohol beer assortments, and beers with an increased shelf life, by complying with current food safety regulations, under which maize and sorghum can be used in manufacturing recipes.
Acute Angle-Closure—A Rare but Vision-Threatening Ocular Adverse Effect of Selective Serotonin Reuptake Inhibitors
Authors: Wiciński Michał , Kaluzny Bartlomiej J. , Liberski Sławomir , Marczak Daria , Kaluzny Jakub J.
Published: April 2021

Acute angle closure (AAC) is a relatively rare but serious ophthalmological condition in which early diagnosis and intraocular pressure (IOP) -lowering treatment play a key role in prognosis. The aim of this review is to present current understanding of the pathophysiology, symptoms, diagnosis, potential mechanisms as well as management of AAC associated with the use of selective serotonin reuptake inhibitors (SSRIs). In most cases, AAC in individuals receiving SSRIs occurs in the pupillary block mechanism, secondary to SSRI-induced mydriasis. However, SSRIs may also cause uveal effusion and, consequently, iridocorneal angle closure. Other factors such as impaired metabolism and elimination of SSRIs, individual genetic conditions, as well as inhibition of SSRIs metabolism due to the effects of other drugs used may also promote AAC.
SPME-GC-MS and FTIR-ATR Spectroscopic Study as a Tool for Unifloral Common Greek Honeys’ Botanical Origin Identification
Authors: Xagoraris Marinos , Revelou Panagiota-Kyriaki , Dedegkika Stela , Kanakis Charalabos D. , Papadopoulos George K. , Pappas Christos S. , Tarantilis Petros A.
Published: April 2021

Among the variants of Greek honey, the most commonly available are pine, fir, thyme, and citrus honey. Samples of the above kinds of honey, identified according to European and Greek legislation, were studied using gas chromatography coupled with mass spectrometry (GC-MS) and the attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopic techniques. Two chemometric models were developed based on statistically significant volatile compounds (octane; 2-phenylacetaldehyde; 1-nonanol; methyl 2-hydroxybenzoate; 2-(4-methylcyclohex-3-en-1-yl); nonanoic acid) and the 1390–945 and 847–803 cm−1 spectral regions, mainly vibrations of fructose and glucose, combined with the stepwise linear discriminant analysis (stepwise LDA) statistical technique. In total, 85.5% of standard samples, and 82.3% through internal validation and 88.5% through external validation, were identified correctly using the GC-MS-stepwise-LDA chemometric model. The corresponding results for the ATR-FTIR-stepwise-LDA chemometric model were 93.5%, 82.5%, and 84.6%. The double validation (internal, external) enhances the robustness of the proposed chemometric models. The developed models are considered statistically equivalent, but FTIR spectroscopy is simple, rapid, and more economical.
DNN-Based FES Control for Gait Rehabilitation of Hemiplegic Patients
Authors: Jung Suhun , Bong Jae Hwan , Kim Seung-Jong , Park Shinsuk
Published: April 2021

In this study, we proposed a novel machine-learning-based functional electrical stimulation (FES) control algorithm to enhance gait rehabilitation in post-stroke hemiplegic patients. The electrical stimulation of the muscles on the paretic side was controlled via deep neural networks, which were trained using muscle activity data from healthy people during gait. The performance of the developed system in comparison with that of a conventional FES control method was tested with healthy human subjects.
Diversity of Mycobiota Associated with the Cereal Cyst Nematode Heterodera filipjevi Originating from Some Localities of the Pannonian Plain in Serbia
Authors: Oro Violeta , Stanisavljevic Rade , Nikolic Bogdan , Tabakovic Marijenka , Secanski Mile , Tosi Solveig
Published: April 2021

Cereals, particularly wheat, are staple food of the people from the Balkans, dating back to the Neolithic age. In Serbia, cereals are predominantly grown in its northern part between 44° and 45.5° N of the Pannonian Plain. One of the most economically important nematodes on wheat is the cereal cyst nematode, Heterodera filipjevi. Cysts of H. filipjevi survive in soil for years and shelter a large number of microorganisms. The aims of this study were to investigate the diversity of mycobiota associated with the cereal cyst nematode H. filipjevi, to infer phylogenetic relationships of the found mycobiota, and to explore the ecological connection between fungi and the field history, including the potential of fungi in bioremediation and the production of novel bioactive compounds. Cysts were isolated from soil samples with a Spears apparatus and collected on a 150-µm sieve. The cysts were placed on potato dextrose agar, and maintained for two weeks at 27°C. Following fungal isolation and colony growing, the fungal DNA was extracted, the ITS region was amplified, and PCR products were sequenced. The study showed that the isolated fungal species belong to diverse phyla, including Ascomycota, Basidiomycota, and Mucoromycota. Ascomycota is represented by the families Clavicipitaceae, Sarocladiaceae, Nectriaceae, and Phaeosphaeriaceae. Basidiomycota is represented by the families Cerrenaceae, Polyporaceae, Phanerochaetaceae, and Meruliaceae, and the order Cantharellales. The family Mortierellaceae represents Mucoromycota. The members of Ascomycota and Basidiomycota both depict the field history. Ascomycota indicate the fungal infection is of recent origin, while Basidiomycota point toward the preceding host plants, enabling the plant field colonization history to be traced chronologically.
The Side Effects of Therapeutic Radioiodine-131 on the Structure of Enamel and Dentin in Permanent Human Teeth
Authors: Mester Alexandru , Moldovan Marioara , Taulescu Marian , Sarosi Codruta , Petean Ioan , Vulpoi Adriana , Piciu Andra , Voina-Tonea Andrada , Moisescu-Goia Cristina , Barbus Elena , Piciu Doina
Published: April 2021

Radioiodine-131 (I-131) is an essential therapy for patients with differentiated thyroid carcinomas (DTC). Generally, I-131 is safe and well tolerated, but patients may present early or late complications in the oral and maxillofacial areas. Thus, the aim of this study was to evaluate in-vitro, the alteration of enamel and dentin after I-131 exposure using histopathological assessment, scanning electron microscopy (SEM) and atomic force microscopy (AFM). For I-131 irradiation, an in-vitro protocol was used that simulates the procedure for irradiation therapy performed for patients with DTCs. A total of 42 teeth were divided into seven groups (n = 6) and irradiated as follows: control, irradiation groups (3, 6, 12, 36, 48 h, 8 days). Histological changes were observed at 48 h (enamel surface with multifocal and irregular areas) and at 8 days (enamel surface with multiple, very deep, delimited cavities). SEM imaging revealed the enamel destruction progresses along with the treatment time increasing. The alterations are extended into the enamel depth and the dislocated hydroxyapatite debris is overwhelming. The enamel-dentine interface shows small gaps after 6 h and a very well developed valley after 12 h; the interface microstructure resulted after 8 days is deeply altered. The AFM imaging shows that I-131 affects the protein bond between hydroxyapatite nano-crystals causing loss of cohesion, which leads to significant increasing of nano-particles diameter after 6 h. In conclusion, both enamel and dentin appear to be altered between 12 and 48 h and after 8 days of treatment are extended in depth.
Mesenchymal Stem Cell-Derived Exosomes and Their Therapeutic Potential for Osteoarthritis
Authors: Kim Gi Beom , Shon Oog-Jin , Seo Min-Soo , Choi Young , Park Wook Tae , Lee Gun Woo
Published: April 2021

Exosomes are nano-sized vesicles (50–150 nm in diameter) that contain nucleic acids (e.g., microRNA and messenger RNA), functional proteins, and bioactive lipids. They are secreted by various types of cells, including B cells, T cells, reticulocytes, dendritic cells, mast cells, epithelial cells, and mesenchymal stem cells (MSCs). They perform a wide variety of functions, including the repair of damaged tissues, regulation of immune responses, and reduction in inflammation. When considering the limitations of MSCs, including issues in standardization and immunogenicity, MSC-derived exosomes have advantages such as small dimensions, low immunogenicity, and lack of requirement for additional procedures for culture expansion or delivery. MSC-derived exosomes have shown outstanding therapeutic effects through chondro-protective and anti-inflammatory properties. MSC-derived exosomes may enable a new therapeutic paradigm for the treatment of osteoarthritis. However, further research is needed to prove their clinical effectiveness and feasibility.
A Novel Wearable Flexible Dry Electrode Based on Cowhide for ECG Measurement
Authors: Huang Yiping , Song Yatong , Gou Li , Zou Yuanwen
Published: April 2021

The electrocardiogram (ECG) electrode, as a sensor, is an important part of the wearable ECG monitoring device. Natural leather is rarely used as the electrode substrate. In this paper, wearable flexible silver electrodes based on cowhide were prepared by sputtering and brush-painting. A signal generator, oscilloscope, impedance test instrument, and ECG monitor were used to build the test platform evaluating the performance of electrodes with six subjects. The lossless waveform transmission can be achieved with our electrodes. Therefore, the Pearson’s correlation coefficient calculated with input waveform and output waveform of the electrodes based on the top grain layer (GLE) and the split layer (SLE) of cowhide were 0.997 and 0.998 at 0.1 Hz respectively. The skin electrode impedance (Z) was tested, and the parameters of the equivalent circuit model of the skin electrode interface were calculated by a fitting method, indicating that the Z of the prepared electrodes was comparable with the standard gel electrode when the skin is moist enough. The signal-to-noise ratio of the ECG of the GLE and the SLE were 1.148 and 1.205 times that of the standard electrode in the standing posture, which meant the ECG measured by our electrodes was basically consistent with that measured by the standard electrode.
Functional Gait Recovery after a Combination of Conventional Therapy and Overground Robot-Assisted Gait Training Is Not Associated with Significant Changes in Muscle Activation Pattern: An EMG Preliminary Study on Subjects Subacute Post Stroke
Authors: Infarinato Francesco , Romano Paola , Goffredo Michela , Ottaviani Marco , Galafate Daniele , Gison Annalisa , Petruccelli Simone , Pournajaf Sanaz , Franceschini Marco
Published: April 2021

Background: Overground Robot-Assisted Gait Training (o-RAGT) appears to be a promising stroke rehabilitation in terms of clinical outcomes. The literature on surface ElectroMyoGraphy (sEMG) assessment in o-RAGT is limited. This paper aimed to assess muscle activation patterns with sEMG in subjects subacute post stroke after training with o-RAGT and conventional therapy. Methods: An observational preliminary study was carried out with subjects subacute post stroke who received 15 sessions of o-RAGT (5 sessions/week; 60 min) in combination with conventional therapy. The subjects were assessed with both clinical and instrumental evaluations. Gait kinematics and sEMG data were acquired before (T1) and after (T2) the period of treatment (during ecological gait), and during the first session of o-RAGT (o-RAGT1). An eight-channel wireless sEMG device acquired in sEMG signals. Significant differences in sEMG outcomes were found in the BS of TA between T1 and T2. There were no other significant correlations between the sEMG outcomes and the clinical results between T1 and T2. Conclusions: There were significant functional gains in gait after complex intensive clinical rehabilitation with o-RAGT and conventional therapy. In addition, there was a significant increase in bilateral symmetry of the Tibialis Anterior muscles. At this stage of the signals from the tibialis anterior (TA), gastrocnemius medialis (GM), rectus femoris (RF), and biceps femoris caput longus (BF) muscles of each lower extremity. sEMG data processing extracted the Bilateral Symmetry (BS), the Co-Contraction (CC), and the Root Mean Square (RMS) coefficients. Results: Eight of 22 subjects in the subacute stage post stroke agreed to participate in this sEMG study. This subsample demonstrated a significant improvement in the motricity index of the affected lower limb and functional ambulation. The heterogeneity of the subjects’ characteristics and the small number of subjects was associated with high variability research, functional gait recovery was associated with minimal change in muscle activation patterns.
Executive Functions and Domain-Specific Cognitive Skills in Climbers
Authors: Heilmann Florian
Published: April 2021

Athletes in a particular sport have specific cognitive skills acquired due to regular confrontation with sport-specific requirements. Studies show that the particular type of sport carried out and fostered by general physical activity impacts executive functions (EFs) such as inhibition, working memory, and cognitive flexibility. There are inconsistent results on the connections between domain-specific cognitive skills and executive functions. This study aimed to evaluate the relations between EFs and domain-specific cognitive skills in climbing. Due to that, we examined the executive functions (neuropsychological tests) and domain-specific cognitive skills (climbing-specific test: a preview of the route vs. climbed moves; climbed moves vs. recognition of moves) of 19 climbers (10 novices, 9 experts, grades 5 to 6a vs. 6c+ to 7b). The inter-subject effects analysis shows that novices and experts in sport climbing do not differ in executive functions in this particular case. Concerning domain-specific cognitive skills, there are differences between experts and novices. Experts show a significantly higher level in planning performance or route idea (p < 0.001) as well as in memorizing of climbed moves (p = 0.004). There are no relations between executive functions and domain-specific cognitive skills in climbers.
A Simplified Approach for the Seismic Loss Assessment of RC Buildings at Urban Scale: The Case Study of Potenza (Italy)
Authors: Flora Amedeo , Cardone Donatello , Vona Marco , Perrone Giuseppe
Published: April 2021

Comprehensive methodologies based on a fully probabilistic approach (i.e., the performance-based earthquake engineering approach, PBEE), represent a refined and accurate tool for the seismic performance assessment of structures. However, those procedures are suitable for building-specific evaluations, appearing extremely time-consuming if applied at the urban scale. In the proposed contribution, simplified loss assessment procedure will be applied at the urban scale with reference to the residential building stock of the center of Potenza. After the identification of the main reinforced concrete (RC) structural typologies and the definition of specific archetype building numerical models, the direct estimation of expected annual loss (DEAL) methodology will be applied to derive the EAL (i.e., expected annual loss) of RC buildings, deriving information on the effectively seismic quality (or seismic resilience) of the aforementioned built heritage at urban scale. Similarly, the monetary losses associated with downtime are evaluated. Preliminary considerations on the socio-economic effects of seismic scenarios on the territorial scale are also proposed.
Improving Post-Disaster Housing Reconstruction Outcomes in the Global South: A Framework for Achieving Greater Beneficiary Satisfaction through Effective Community Consultation
Authors: Capell Troy , Ahmed Iftekhar
Published: April 2021

The purpose of this paper was to examine the community consultation practices carried out by implementing agencies when managing post-disaster housing reconstruction (PDHR) projects, identify the obstacles they face, and evaluate the effect these obstacles have on the quality of information obtained in relation to achieving a high level of beneficiary satisfaction. The aim was to develop a framework for community consultation in PDHR, which ensures that the needs of beneficiaries are more accurately reflected in the housing produced. A qualitative research methodology was adopted with data first being obtained through a literature review of relevant publications and implementing agency reports as a means of establishing common themes among community consultation practices and identifying indicators that influence beneficiary satisfaction. This was followed by a case study analysis to further an understanding of how these indicators were affected by the community consultation practices undertaken in a real-world context. The results of the research indicate that community consultation often forms a central role in the planning and delivery of PDHR projects, however, despite the implementing agencies’ best intentions, there are often obstacles that prevent the process from achieving the desired result. The community consultation framework proposed here has been developed to address the identified obstacles with the aim of ensuring that beneficiary requirements are included in housing design as a means of improving the level of beneficiary satisfaction in the housing provided.
Quality of Life with Vulvar Carcinoma Treated with Palliative Electrochemotherapy: The ELECHTRA (ELEctroCHemoTherapy vulvaR cAncer) Study
Authors: Perrone Anna Myriam , Ferioli Martina , Argnani Lisa , De Terlizzi Francesca , Pirovano Cecilia , Covarelli Piero , Dondi Giulia , Tesei Marco , De Crescenzo Eugenia , Ravegnini Gloria , Galuppi Andrea , Morganti Alessio G. , De Iaco Pierandrea
Published: April 2021

The ELECHTRA (ELEctroChemoTherapy vulvaR cAncer) project was conceived to collect data on palliative electrochemotherapy (ECT) in vulvar cancer (VC) assessing patients’ outcomes (response and survival) and impact on quality of life (QoL). After reporting outcome data in 2019, here, we present the results on QoL. A multicenter prospective observational study was conducted on patients with VC refractory or not amenable to standard therapies undergoing palliative ECT as per clinical practice. The following questionnaires were administered before and after ECT (two and four months later, early and late follow-up): visual analog pain scale (VAS), EuroQol 5-Dimension 5-Level (EQ-5D-L5) and Functional Assessment of Cancer Therapy—Vulva cancer (FACT—V). Analyses were conducted on both the whole study population and by subgroups (clinical response after ECT and site, number and size of lesions). Questionnaires from 55 patients were evaluated. Compared to the baseline (6.1 ± 2.1), the VAS was significantly reduced at early (4.3 ± 2.5) and late follow-up (4.6 ± 2.8) (p < 0.0001). The FACT—V score improved significantly at early (9.6 ± 4.0) (p < 0.0001) and late follow-up (8.9 ± 4.1) (p < 0.0054) as compared to the baseline (7.1 ± 3.6). No EQ-5D-5L statistically significant changes were observed. Subgroup analyses showed worse QoL in patients with stable or progressive disease, posterior site and multiple or larger than 3 cm nodules. This is the first study reporting improved QoL in VC patients after palliative ECT. Based on these results, ECT in VC should be considered an effective option based on the favorable outcomes both in terms of response and QoL.
Efficacy and Safety of Checkpoint Inhibitor Treatment in Patients with Advanced Renal or Urothelial Cell Carcinoma and Concomitant Chronic Kidney Disease: A Retrospective Cohort Study
Authors: Seydel Florian , Delecluse Susanne , Zeier Martin , Holland-Letz Tim , Haag Georg Martin , Berger Anne Katrin , Grün Barbara Christine , Bougatf Nina , Hohenfellner Markus , Duensing Stefan , Jäger Dirk , Zschäbitz Stefanie
Published: April 2021

Background: Checkpoint inhibitors are a standard of care in the treatment of advanced renal cell carcinoma (RCC) and urothelial carcinoma (UC). Patients with these tumors often suffer from concomitant chronic kidney disease (CKD). Limited data are available on the efficacy and toxicity of checkpoint inhibitors in patients with CKD. Methods: We retrospectively analyzed 126 patients who received checkpoint inhibitors for RCC (n = 85) or UC (n = 41) and analyzed the frequency of treatment- and immune-related adverse events (AEs). We performed a multivariate analysis to determine progression-free survival (PFS) and overall survival (OS). Results: A total of 38.9% of patients had CKD. Frequencies of general AEs (49.0% in CKD vs. 48.1%, p > 0.99999) and immune-related AEs (28.6 vs. 24.7%, p ≥ 0.9999) did not significantly differ between the groups. There was no difference in PFS for patients with RCC or UC and CKD or without CKD (RCC: 6.81 vs. 7.54 months, HR 1.000 (95%CI 0.548–01.822), p = 0.999; UC:2.33 vs. 3.67 months, HR 01.492 (95%CI 0.686–3.247), p = 0.431). CKD appeared to be a potential effect modifier for OS in both RCC and UC (RCC: NR vs. 23.9 months, HR 0.502 (95%CI 0.219–1.152), p = 0.104; UC:18.84 vs. 15.42 months, HR 0.656 (95%CI 0.296–1.454), p = 0.299). Conclusions: Checkpoint inhibitor treatment in our cohort of patients with CKD was as safe and efficient as in the cohort of patients without CKD.
Assessment of Immunological Features in Muscle-Invasive Bladder Cancer Prognosis Using Ensemble Learning
Authors: Gavriel Christos G. , Dimitriou Neofytos , Brieu Nicolas , Nearchou Ines P. , Arandjelović Ognjen , Schmidt Günter , Harrison David J. , Caie Peter D.
Published: April 2021

The clinical staging and prognosis of muscle-invasive bladder cancer (MIBC) routinely includes the assessment of patient tissue samples by a pathologist. Recent studies corroborate the importance of image analysis in identifying and quantifying immunological markers from tissue samples that can provide further insight into patient prognosis. In this paper, we apply multiplex immunofluorescence to MIBC tissue sections to capture whole-slide images and quantify potential prognostic markers related to lymphocytes, macrophages, tumour buds, and PD-L1. We propose a machine-learning-based approach for the prediction of 5 year prognosis with different combinations of image, clinical, and spatial features. An ensemble model comprising several functionally different models successfully stratifies MIBC patients into two risk groups with high statistical significance (p value < 1×10−5). Critical to improving MIBC survival rates, our method correctly classifies 71.4% of the patients who succumb to MIBC, which is significantly more than the 28.6% of the current clinical gold standard, the TNM staging system.
Plasma-Activated Medium Potentiates the Immunogenicity of Tumor Cell Lysates for Dendritic Cell-Based Cancer Vaccines
Authors: Tomić Sergej , Petrović Anđelija , Puač Nevena , Škoro Nikola , Bekić Marina , Petrović Zoran Lj. , Čolić Miodrag
Published: April 2021

Autologous dendritic cells (DCs)-based vaccines are considered quite promising for cancer immunotherapy due to their exquisite potential to induce tumor antigen-specific cytotoxic T cells. However, a lack of efficient protocols for inducing immunogenic tumor antigens limits the efficacy of DC-based cancer vaccines. Here, we found that a plasma-activated medium (PAM) induces immunogenic cell death (ICD) in tumor cells but not in an immortalized L929 cell line or human peripheral blood mononuclear cells. PAM induced an accumulation of reactive oxygen species (ROS), autophagy, apoptosis, and necrosis in a concentration-dependent manner. The tumor lysates prepared after PAM treatment displayed increased immunogenicity in a model of human monocyte-derived DCs, compared to the lysates prepared by a standard freezing/thawing method. Mature DCs loaded with PAM lysates showed an increased maturation potential, as estimated by their increased expression of CD83, CD86, CD40, IL-12/IL-10 production, and attenuated PDL1 and ILT-4 expression, compared to the DCs treated with control tumor lysates. Moreover, in co-culture with allogeneic T cells, DCs loaded with PAM-lysates increased the proportion of cytotoxic IFN-γ+ granzyme A+ CD8+ T cells and IL-17A-producing T cells and preserved the Th1 response. In contrast, control tumor lysates-treated DCs increased the frequency of Th2 (CD4+IL-4+), CD4, and CD8 regulatory T cell subtypes, none of which was observed with DCs loaded with PAM-lysates. Cumulatively, these results suggest that the novel method for preparing immunogenic tumor lysates with PAM could be suitable for improved DC-based immunotherapy of cancer patients.
Are We Benign? What Can Wnt Signaling Pathway and Epithelial to Mesenchymal Transition Tell Us about Intracranial Meningioma Progression
Authors: Bukovac Anja , Kafka Anja , Raguž Marina , Brlek Petar , Dragičević Katarina , Müller Danko , Pećina-Šlaus Nives
Published: April 2021

Epithelial to mesenchymal transition (EMT), which is characterized by the reduced expression of E-cadherin and increased expression of N-cadherin, plays an important role in the tumor invasion and metastasis. Classical Wnt signaling pathway has a tight link with EMT and it has been shown that nuclear translocation of β-catenin can induce EMT. This research has showed that genes that are involved in cadherin switch, CDH1 and CDH2, play a role in meningioma progression. Increased N-cadherin expression in relation to E-cadherin was recorded. In meningioma, transcription factors SNAIL, SLUG, and TWIST1 demonstrated strong expression in relation to E- and N-cadherin. The expression of SNAIL and SLUG was significantly associated with higher grades (p = 0.001), indicating their role in meningioma progression. Higher grades also recorded an increased expression of total β-catenin followed by an increased expression of its active form (p = 0.000). This research brings the results of genetic and protein analyzes of important molecules that are involved in Wnt and EMT signaling pathways and reveals their role in intracranial meningioma. The results of this study offer guidelines and new markers of progression for future research and reveal new molecular targets of therapeutic interventions.
Metabolic Parameters as Biomarkers of Response to Immunotherapy and Prognosis in Non-Small Cell Lung Cancer (NSCLC): A Real World Experience
Authors: Monaco Lavinia , Gemelli Maria , Gotuzzo Irene , Bauckneht Matteo , Crivellaro Cinzia , Genova Carlo , Cortinovis Diego , Zullo Lodovica , Ammoni Luca Carlofrancesco , Bernasconi Davide Paolo , Rossi Giovanni , Morbelli Silvia , Guerra Luca
Published: April 2021

Immune-checkpoint inhibitors (ICIs) have been proven to have great efficacy in non-small cell lung cancer (NSCLC) as single agents or in combination therapy, being capable to induce deep and durable remission. However, severe adverse events may occur and about 40% of patients do not benefit from the treatment. Predictive factors of response to ICIs are needed in order to customize treatment. The aim of this study is to evaluate the correlation between quantitative positron emission tomography (PET) parameters defined before starting ICI therapy and responses to treatment and patient outcome. We retrospectively analyzed 92 NSCLC patients treated with nivolumab, pembrolizumab or atezolizumab. Basal PET/computed tomography (CT) scan parameters (whole-body metabolic tumor volume—wMTV, total lesion glycolysis—wTLG, higher standardized uptake volume maximum and mean—SUVmax and SUVmean) were calculated for each patient and correlated with outcomes. Patients who achieved disease control (complete response + partial response + stable disease) had significantly lower MTV median values than patients who had not (progressive disease) (77 vs. 160.2, p = 0.039). Furthermore, patients with MTV and TLG values lower than the median values had improved OS compared to patients with higher MTV and TLG (p = 0.03 and 0.05, respectively). No relation was found between the other parameters and outcome. In conclusion, baseline metabolic tumor burden, measured with MTV, might be an independent predictor of treatment response to ICI and a prognostic biomarker in NSCLC patients.