bluuebunny/crossref_metadata_2025 huggingface dataset:
Partial Slip Effects for Thermally Radiative Convective Nanofluid Flow
Authors: Ene Remus-Daniel , Pop Nicolina , Badarau Rodica
Published: May 2023

The partial slip effects for radiative convective nanofluid flow over a stretching sheet in porous medium are analytically explored in this work. The Navier–Stokes equations, the momentum and the energy equations are converted into a set of non-linear ODEs by the similarity transformation. Using the modified optimal homotopy asymptotic method (OHAM), the resulting non-linear ODEs are analytically approximately solved. The impact of various parameters, such as: the velocity exponential factor n, the wall thickness parameter γ, the dimensionless velocity slip parameter δ1, the Prandtl number Pr, the radiation parameter R, and the dimensionless temperature jump parameter δ2, on the behaviour of the mass and heat transfer is presented. The influence of these parameters is tabular and graphically presented. An excellent agreement between the approximate analytical solution and the corresponding numerical solution is highlighted. The results obtained confirm that modified OHAM is a useful and competitive mathematical tool to explore a large class of non-linear problems with applications in various fields of science and engineering.
Saturated (n, m)-Regular Semigroups
Authors: Alali Amal S. , Bano Sakeena , Nabi Muneer
Published: May 2023

The aim of this paper is to determine several saturated classes of structurally regular semigroups. First, we show that structurally (n,m)-regular semigroups are saturated in a subclass of semigroups for any pair (n,m) of positive integers. We also demonstrate that, for all positive integers n and k with 1≤k≤n, the variety of structurally (0,n)-left seminormal bands is saturated in the variety of structurally (0,k)-bands. As a result, in the category of structurally (0,k)-bands, epis from structurally (0,n)-left seminormal bands is onto.
On Targeted Control over Trajectories of Dynamical Systems Arising in Models of Complex Networks
Authors: Ogorelova Diana , Sadyrbaev Felix , Samuilik Inna
Published: May 2023

The question of targeted control over trajectories of systems of differential equations encountered in the theory of genetic and neural networks is considered. Examples are given of transferring trajectories corresponding to network states from the basin of attraction of one attractor to the basin of attraction of the target attractor. This article considers a system of ordinary differential equations that arises in the theory of gene networks. Each trajectory describes the current and future states of the network. The question of the possibility of reorienting a given trajectory from the initial state to the assigned attractor is considered. This implies an only partial control of the network. The difficulty lies in the selection of parameters, the change of which leads to the goal. Similar problems arise when modeling the response of the body’s gene networks to serious diseases (e.g., leukemia). Solving such problems is the first step in the process of applying mathematical methods in medicine and pharmacology.
Quarter-Symmetric Metric Connection on a Cosymplectic Manifold
Authors: Maksimović Miroslav D. , Zlatanović Milan Lj.
Published: May 2023

We study the quarter-symmetric metric A-connection on a cosymplectic manifold. Observing linearly independent curvature tensors with respect to the quarter-symmetric metric A-connection, we construct the Weyl projective curvature tensor on a cosymplectic manifold. In this way, we obtain new conditions for the manifold to be projectively flat. At the end of the paper, we define η-Einstein cosymplectic manifolds of the θ-th kind and prove that they coincide with the η-Einstein cosymplectic manifold.
Note on Discovering Doily in PG(2,5)
Authors: Innamorati Stefano
Published: May 2023

W. L. Edge proved that the internal points of a conic in PG(2,5), together with the collinear triples on the non-secant lines, form the Desargues configuration. M. Saniga showed an intimate connection between Desargues configurations and the generalized quadrangles of order 2, GQ(2,2), whose representation was dubbed “the doily” by Stan Payne in 1973. In this note, we prove that the external points of a conic in PG(2,5), together with the collinear and non-collinear triples on the non-tangent lines, form the generalized quadrangle of order 2.
Anti-Leukemia Activity of Polysaccharide from Sargassum fusiforme via the PI3K/AKT/BAD Pathway In Vivo and In Vitro
Authors: Du Haofei , Jin Xudong , Jin Sizhou , Zhang Donglei , Chen Qiande , Jin Xuanan , Wang Caisheng , Qian Guoying , Ding Haomiao
Published: May 2023

Studies have shown that Sargassum fusiforme and its extracts are effective herbal treatments for leukemia. We previously found that a polysaccharide from Sargassum fusiforme, SFP 2205, stimulated apoptosis in human erythroleukemia (HEL) cells. However, the structural characterization and antitumoral mechanisms of SFP 2205 remain uncertain. Here, we studied the structural characteristics and anticancer mechanisms of SFP 2205 in HEL cells and a xenograft mouse model. The results demonstrated that SFP 2205, with a molecular weight of 41.85 kDa, consists of mannose, rhamnose, galactose, xylose, glucose, and fucose with monosaccharides composition of 14.2%, 9.4%, 11.8%, 13.7%, 11.0%, and 38.3%, respectively. On animal assays, SFP 2205 significantly inhibited growth of HEL tumor xenografts with no discernible toxicity to normal tissues. Western blotting showed that SFP 2205 therapy improved Bad, Caspase-9, and Caspase-3 protein expression, and ultimately induced HEL tumor apoptosis, indicating mitochondrial pathway involvement. Furthermore, SFP 2205 blocked the PI3K/AKT signaling pathway and 740 Y-P, an activator of the PI3K/AKT pathway, rescued the effects of SFP 2205 on HEL cell proliferation and apoptosis. Overall, SFP 2205 may be a potential functional food additive or adjuvant for preventing or treating leukemia.
Assessment of CA-125 First-Trimester Values as a Potential Screening Marker for Pre-Eclampsia
Authors: Balint Oana , Secosan Cristina , Pirtea Laurentiu
Published: May 2023

Background: Pre-eclampsia is a major public health issue. Current screening methods are based on maternal characteristics and medical history, but complex predictive models combining different clinical and biochemical markers have been proposed. However, although their accuracy is high, the implementation of these models in clinical practice is not always feasible, especially in low- and middle-resource settings. CA-125 is a tumoral marker, accessible and cheap, with proven potential as a severity marker in the third trimester of pregnancy in pre-eclamptic women. Assessment of its use as a first-trimester marker is necessary. Methods: This observational study involved fifty pregnant women between 11 and 14 weeks of pregnancy. Clinical and biochemical markers (PAPP-A), known for their value in pre-eclampsia screening, were recorded for every patient as well as first-trimester value of CA-125 and third-trimester data regarding blood pressure and pregnancy outcome. Results: No statistical correlation between CA-125 and first-trimester markers was observed except with PAPP-A, with which it exhibited a positive correlation. Additionally, no correlation was made between it and third-trimester blood pressure or pregnancy outcomes. Conclusions: CA-125 first-trimester values do not represent a valuable marker for pre-eclampsia screening. Further research on identifying an accessible and cheap marker to improve pre-eclampsia screening in low- and middle-income settings is needed.
Pathological Neurovascular Unit Mapping onto Multimodal Imaging in Diabetic Macular Edema
Authors: Murakami Tomoaki , Ishihara Kenji , Terada Noriko , Nishikawa Keiichi , Kawai Kentaro , Tsujikawa Akitaka
Published: May 2023

Diabetic retinopathy is a form of diabetic microangiopathy, and vascular hyperpermeability in the macula leads to retinal thickening and concomitant reduction of visual acuity in diabetic macular edema (DME). In this review, we discuss multimodal fundus imaging, comparing the pathogenesis and interventions. Clinicians diagnose DME using two major criteria, clinically significant macular edema by fundus examination and center-involving diabetic macular edema using optical coherence tomography (OCT), to determine the appropriate treatment. In addition to fundus photography, fluorescein angiography (FA) is a classical modality to evaluate morphological and functional changes in retinal capillaries, e.g., microaneurysms, capillary nonperfusion, and fluorescein leakage. Recently, optical coherence tomography angiography (OCTA) has allowed us to evaluate the three-dimensional structure of the retinal vasculature and newly demonstrated that lamellar capillary nonperfusion in the deep layer is associated with retinal edema. The clinical application of OCT has accelerated our understanding of various neuronal damages in DME. Retinal thickness measured by OCT enables us to quantitatively assess therapeutic effects. Sectional OCT images depict the deformation of neural tissues, e.g., cystoid macular edema, serous retinal detachment, and sponge-like retinal swelling. The disorganization of retinal inner layers (DRIL) and foveal photoreceptor damage, biomarkers of neurodegeneration, are associated with visual impairment. Fundus autofluorescence derives from the retinal pigment epithelium (RPE) and its qualitative and quantitative changes suggest that the RPE damage contributes to the neuronal changes in DME. These clinical findings on multimodal imaging help to elucidate the pathology in the neurovascular units and lead to the next generation of clinical and translational research in DME.
The Impact of Spray Cryotherapy on Lesion-Induced Osteitis in a New Murine Experimental Model
Authors: Porfire (Irimia) Ioana Maria , Tabaran Alexandru Flaviu , Gherman Madalina Luciana , Trombitas Veronica Elena , Albu Silviu
Published: May 2023

Background and Objectives: Endoscopic sinus surgery is considered the gold management strategy for difficult-to-treat chronic rhinosinusitis. The inflammatory bony process is incriminated as being involved in the unfavorable evolution and recurrence of the disease. Osteitis is significantly increased in patients that have been previously submitted to surgery, and it is more often present in patients with extended radiological disease and in patients undergoing revision surgery. The aim of the research is to demonstrate the presence of inflammations and neo-osteogenesis associated with nasal mucosal surgical injury and the correlation between their severity and to evaluate the efficacy of low-pressure spray cryotherapy in reducing inflammation and bone remodeling. Materials and Methods: The experimental murine model was conducted over a period of 80 days and included a total of 60 adult female Wistar rats, with three periods of withdrawal of 20 individuals each from the experiment. After inducing a bilateral mechanical injury by brushing, low-pressure spray cryotherapy application was performed unilaterally, and tissue samples were prepared specifically for histological analysis. The scores for inflammation and osteitis were compared over time and between the two nasal fossae. Results: Osteitis and inflammation were induced by a simple mucosal brushing lesion, similar to surgical injury. We identified the presence of inflammation in 95% of the specimens, and it was present over time. Moreover, criteria for bone remodeling were clearly highlighted in a percentage of 72% of the specimens. There was a direct correlation between the severity of inflammation and neo-osteogenesis, with a statistical significance of p = 0.050. Low-pressure spray cryotherapy was safe and effective in reducing inflammation (p = 0.020) and osteitis (p = 0.000) with a safety profile. Conclusions: Low-pressure cryotherapy reduces the severity of mucosal inflammation and osteitis in lesion-induced neo-osteogenesis.
Eco-Friendly Electroless Template Synthesis of Cu-Based Composite Track-Etched Membranes for Sorption Removal of Lead(II) Ions
Authors: Altynbaeva Liliya Sh. , Mashentseva Anastassiya A. , Aimanova Nurgulim A. , Zheltov Dmitriy A. , Shlimas Dmitriy I. , Nurpeisova Dinara T. , Barsbay Murat , Abuova Fatima U. , Zdorovets Maxim V.
Published: May 2023

This paper reports the synthesis of composite track-etched membranes (TeMs) modified with electrolessly deposited copper microtubules using copper deposition baths based on environmentally friendly and non-toxic reducing agents (ascorbic acid (Asc), glyoxylic acid (Gly), and dimethylamine borane (DMAB)), and comparative testing of their lead(II) ion removal capacity via batch adsorption experiments. The structure and composition of the composites were investigated by X-ray diffraction technique and scanning electron and atomic force microscopies. The optimal conditions for copper electroless plating were determined. The adsorption kinetics followed a pseudo-second-order kinetic model, which indicates that adsorption is controlled by the chemisorption process. A comparative study was conducted on the applicability of the Langmuir, Freundlich, and Dubinin–Radushkevich adsorption models to define the equilibrium isotherms and the isotherm constants for the prepared composite TeMs. Based on the regression coefficients R2, it has been shown that the Freundlich model better describes the experimental data of the composite TeMs on the adsorption of lead(II) ions.
Difference Discretization Method for Milling Stability Prediction
Authors: Wang Chenxi
Published: May 2023

This paper proposes a difference discretization method (DDM) under a difference frame for the prediction of milling stability. In this method, the dynamic milling process is described by a delay differential equation (DDE) with two degrees of freedom rather than the traditional state-space form with a single discrete time delay. After discretization, only the velocity and acceleration in the DDE are approximated by the first- and second-order central difference for each smaller time interval, while the other items are kept unchanged. Then, the criterion for the optimal discretization interval number is put forward and derived based on the largest effective time interval (also called the critical time interval). The use of the critical time interval cannot only obtain sufficient accuracy, but also promotes as much efficiency as possible. Subsequently, a new DDM (NDDM) with varied discretization interval numbers as the milling rotating speeds is developed. Finally, the effectiveness of the proposed algorithm is demonstrated by using a benchmark example for a two-degrees-of- freedom milling model compared to the full discretization method (FDM) and the Hermite-interpolation full discretization method (HFDM). The results show that the proposed method has satisfactory stability charts and is able to increase the efficiency by 100% or more.
Prediction Model of Strip Crown in Hot Rolling Process Based on Machine Learning and Industrial Data
Authors: Wang Zhenhua , Huang Yu , Liu Yuanming , Wang Tao
Published: May 2023

The strip crown in hot rolling has the characteristics of multivariablity, strong coupling and, nonlinearity. It is difficult to describe accurately using a traditional mechanism model. In this paper, based on the industrial data of a hot continuous rolling field, the modeling dataset of a strip steel prediction model is constructed through the collection and collation of the on-site data. According to the classical strip crown control theory, the important process parameters that affect the strip crown are determined as input variables for the data-driven model. Some new intelligent strip crown prediction models integrating the shape control mechanism model, artificial intelligence algorithm, and production data are constructed using four machine learning algorithms, including XGBoost, Random Forest (RF), Support Vector Machine (SVM), and Multi-Layer Perceptron (MLP). The overall performance of the models is evaluated using error indicators, such as Mean Absolute Percentage Error (MAPE), Root Mean square Error (RMSE), and Mean Absolute Error (MAE). The research results showed that, for the test set, the determination coefficient (R2) of the predicted value of the strip crown model based on the XGBoost algorithm reached 0.971, and the three error indexes are at the lowest level, meaning that the overall model has the optimal generalization performance, which can realize the accurate prediction of the outlet strip crown in the hot rolling process. The research results can promote the application of industrial data and machine learning modeling to the actual strip shape control process of hot rolling, and also have important practical value for the intelligent preparation of the whole process of steel.
Effect of Proton Irradiation on Zr/Nb Nanoscale Multilayer Structure and Properties
Authors: Laptev Roman , Krotkevich Dmitriy , Lomygin Anton , Stepanova Ekaterina , Pushilina Natalia , Kashkarov Egor , Doroshkevich Aleksandr , Sidorin Alexey , Orlov Oleg , Uglov Vladimir
Published: May 2023

The effect of proton irradiation on the structure, phase composition, defect state and nanohardness of Zr/Nb nanoscale multilayer coatings was investigated. Preservation of the Zr/Nb layered structure with 50 and 100 nm thick layers, was observed after irradiation with protons at 1720 keV energy and 3.4 × 1015, 8.6 × 1015 and 3.4 × 1016 ions/cm2 fluences, and the interfaces remained incoherent. In the Zr/Nb nanoscale multilayer coatings with individual layer thicknesses of 10 and 25 nm, there were insignificant fluctuations in interplanar distance, which were influenced by changes in irradiation fluence, and the interfaces were partially destroyed and became semicoherent. Changing irradiation fluence in the investigated ranges led to a decrease in the nanohardness of the Zr/Nb nanoscale multilayer coatings with individual layer thicknesses of 10–50 nm. Variable-energy positron Doppler broadening analysis revealed that these changes are primarily caused by peculiarities of the localization and accumulation of the embedded ions and do not cause a significant increase in the S-parameters of Zr/Nb nanoscale multilayer coatings with a layer thickness less than 100 nm.
In Situ Synchrotron HEXRD Study on the Deformation Mechanism of a Nickel-Based Superalloy during Medium-Temperature Compression
Authors: Wang Hao , Tong Ruolan , Shang Huashan , Sha Aixue , Liu Guangxu , Song Lin , Zhang Tiebang
Published: May 2023

The γ′ phase has an important influence on the deformation mechanism of solid-solution strengthening nickel-based superalloys. The microfracture behavior of the alloy depends on the mechanism of stress and strain partitioning between the γ and γ′ phase under load. In this study, the in situ synchrotron radiation high-energy X-ray diffraction technique was used to observe the deformation process of the FGH96 nickel-based superalloy with a γ′ volume fraction of about 40% at 650 °C and 750 °C. The results show that the (111) reflection had the greatest stiffness and showed plastic deformation first; while the (200) reflection bore a larger load. The γ phase yielded first and began to deform plastically; then the load was transferred to the γ′ phase. At 650 °C, the plastic deformation of the γ′ phase was relatively higher; while at 750 °C, the γ′ particle basically maintained elastic deformation with a tiny amount of plastic deformation.
Die Design and Finite Element Analysis of Welding Seams during Aluminum Alloy Tube Extrusion
Authors: Hwang Yeong-Maw , Hsu I-Peng
Published: May 2023

Hollow tubes are generally manufactured using porthole die extrusion. A finite element software QForm is used to analyze the material flow of aluminum alloy A6061 tubes inside a specially designed porthole die during tube extrusion. High welding pressure and shorter transverse seam length are required for a sound product. Various extrusion conditions and die geometries and dimensions affect the bonding strength of the products. In this paper, the effects of die geometries on the welding pressure are discussed using the Taguchi method. The simulation results show that a higher welding pressure is obtained with a larger porthole radius, a larger welding chamber height, and a larger bearing length, while a larger bridge width increases the welding pressure slightly. For transverse seam lengths, a shorter transverse seam length can be obtained with a smaller porthole radius and a smaller welding chamber height, and a shorter bridge width and bearing length decrease the transverse seam length slightly. The transverse seam region and flow patterns are observed. Tube expanding tests were also conducted. From the expanding test results, it is known that the fracture position did not occur at the welding line and the bonding strength could reach up to 160 MPa.
Potential Benefits of Omega-3 Polyunsaturated Fatty Acids (N3PUFAs) on Cardiovascular Health Associated with COVID-19: An Update for 2023
Authors: Lu Louise Weiwei , Quek Siew-Young , Lu Shi-Ping , Chen Jie-Hua
Published: May 2023

The accumulating literature demonstrates that omega-3 polyunsaturated fatty acid (n-3 polyunsaturated fatty acid, N3PUFA) can be incorporated into the phospholipid bilayer of cell membranes in the human body to positively affect the cardiovascular system, including improving epithelial function, decreasing coagulopathy, and attenuating uncontrolled inflammatory responses and oxidative stress. Moreover, it has been proven that the N3PUFAs, eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), are precursors of some potent endogenous bioactive lipid mediators that mediate some favorable effects attributed to their parent substances. A dose–response relationship between increased EPA and DHA intake and reduced thrombotic outcomes has been reported. The excellent safety profile of dietary N3PUFAs makes them a prospective adjuvant treatment for people exposed to a higher risk of cardiovascular problems associated with COVID-19. This review presented the potential mechanisms that might contribute to the beneficial effects of N3PUFA and the optimal form and dose applied.
Cerebral Glutamate Alterations Using Chemical Exchange Saturation Transfer Imaging in a Rat Model of Lipopolysaccharide-Induced Sepsis
Authors: Lee Do-Wan , Kwon Jae-Im , Heo Hwon , Woo Chul-Woong , Yu Na Hee , Kim Kyung Won , Woo Dong-Cheol
Published: May 2023

Glutamate-weighted chemical exchange saturation transfer (GluCEST) is a useful imaging tool to detect glutamate signal alterations caused by neuroinflammation. This study aimed to visualize and quantitatively evaluate hippocampal glutamate alterations in a rat model of sepsis-induced brain injury using GluCEST and proton magnetic resonance spectroscopy (1H-MRS). Twenty-one Sprague Dawley rats were divided into three groups (sepsis-induced groups (SEP05, n = 7 and SEP10, n = 7) and controls (n = 7)). Sepsis was induced through a single intraperitoneal injection of lipopolysaccharide (LPS) at a dose of 5 mg/kg (SEP05) or 10 mg/kg (SEP10). GluCEST values and 1H-MRS concentrations in the hippocampal region were quantified using conventional magnetization transfer ratio asymmetry and a water scaling method, respectively. In addition, we examined immunohistochemical and immunofluorescence staining to observe the immune response and activity in the hippocampal region after LPS exposure. The GluCEST and 1H-MRS results showed that GluCEST values and glutamate concentrations were significantly higher in sepsis-induced rats than those in controls as the LPS dose increased. GluCEST imaging may be a helpful technique for defining biomarkers to estimate glutamate-related metabolism in sepsis-associated diseases.
Evaluation of Rumen Methane Emission in Sahiwal and Gir Calves Supplemented with Combination of Methanogenic Inhibitors
Authors: Reddy Rachala Dinesh , Chaudhary Parul , Tyagi Nitin , Mohini Madhu , Mondal Goutam
Published: May 2023

Methane is one of the main greenhouse gases emitted by ruminants around the world. It is essential to investigate novel approaches to increasing animal production while reducing greenhouse gas emissions from ruminants. This study was conducted to examine the effect of methane inhibitors, such as nitrate, linseed oil, and anthraquinone, on nutritional digestibility, rumen fermentation processes, and methane emission in Sahiwal and Gir cattle calves. Twelve calves (6–12 months old), six of each Sahiwal and Gir breed, were selected and divided into four groups; Sahiwal control (C) and treated (T) calves; Gir control (C) and treated calves (T) of three calves each based on average body weight. Switch over a design was used as for periods 1 and 2. Animals in all groups were fed chopped oat fodder, wheat straw, and a concentrate mixture. Additionally, treated groups were fed a ration with potassium nitrate (1%), linseed oil (0.5%), and anthraquinone (4 ppm). The results revealed that the addition of methane inhibitors had no impact on nutrient intake and apparent digestibility. The levels of propionate, ammonia nitrogen, and total nitrogen were increased significantly (p < 0.05), while butyrate decreased in the treated groups of both breeds. However, there was no change in acetate and pH between the groups. Methane emission (g/d) was lower (p < 0.05) in the treated groups as compared to the control group. This study concludes that supplementation of methane inhibitors in calves feed can be utilized to lower methane emissions without affecting the intake and digestibility of nutrients. Combining diverse dietary mitigation strategies could be an effective way to mitigate methane emissions to reduce global warming while minimizing any negative impacts on ruminants to accomplish sustainable animal production.
A Wire Bow Model of Diamond Wire Sawing with Asymmetric Arc Hypothesis
Authors: Dong Zhikui , Zhang Chenpu , Liu Ziliang , Zhao Yanheng , Xing Ke , Guo Wenming
Published: May 2023

Diamond wire sawing is the main processing method for hard and brittle materials, but the unreasonable matching of process parameters will reduce its cutting ability and stability. In this paper, the asymmetric arc hypothesis of a wire bow model is proposed. Based on this hypothesis, an analytical model of the wire bow between the process parameters and the wire bow parameters was established and verified with a single-wire cutting experiment. The model considers the asymmetry of the wire bow in diamond wire sawing. The tension at both ends of the wire bow is called the endpoint tension; by calculating the difference in tension between the two ends, a reference for the cutting stability and a tension range for the selection of the diamond wire were provided. The model was used to calculate the wire bow deflection and the cutting force, providing theoretical guidance for the matching of process parameters. Based on the theoretical analysis of the cutting force, endpoint tension and wire bow deflection, the cutting ability, cutting stability, and the risk of wire cutting were predicted.
Current Progress in Conductive Hydrogels and Their Applications in Wearable Bioelectronics and Therapeutics
Authors: Khan Bangul , Abdullah Saad , Khan Samiullah
Published: May 2023

Wearable bioelectronics and therapeutics are a rapidly evolving area of research, with researchers exploring new materials that offer greater flexibility and sophistication. Conductive hydrogels have emerged as a promising material due to their tunable electrical properties, flexible mechanical properties, high elasticity, stretchability, excellent biocompatibility, and responsiveness to stimuli. This review presents an overview of recent breakthroughs in conductive hydrogels, including their materials, classification, and applications. By providing a comprehensive review of current research, this paper aims to equip researchers with a deeper understanding of conductive hydrogels and inspire new design approaches for various healthcare applications.
Multiband Microstrip Rectenna Using ZnO-Based Planar Schottky Diode for RF Energy Harvesting Applications
Authors: Kayed Somaya I. , Elsheakh Dalia N. , Mohamed Hesham A. , Shawkey Heba A.
Published: May 2023

This paper presents a single-substrate microstrip rectenna for dedicated radio frequency energy harvesting applications. The proposed configuration of the rectenna circuit is composed of a clipart moon-shaped cut in order to improve the antenna impedance bandwidth. The curvature of the ground plane is modified with a simple U-shaped slot etched into it to improve the antenna bandwidth by changing the current distribution; therefore, this affects the inductance and capacitance embedded into the ground plane. The linear polarized ultra-wide bandwidth (UWB) antenna is achieved by using 50 Ω microstrip line and build on Roger 3003 substrate with an area of 32 × 31 mm2. The operating bandwidth of the proposed UWB antenna extended from 3 GHz to 25 GHz at −6 dB reflection coefficient (VSWR ≤ 3) and extended from both 3.5 to 12 GHz, from 16 up to 22 GHz at −10 dB impedance bandwidth (VSWR ≤ 2). This was used to harvest RF energy from most of the wireless communication bands. In addition, the proposed antenna integrates with the rectifier circuit to create the rectenna system. Moreover, to implement the shunt half-wave rectifier (SHWR) circuit, a planar Ag/ZnO Schottky diode uses a diode area of 1 × 1 mm2. The proposed diode is investigated and designed, and its S-parameter is measured for use in the circuit rectifier design. The proposed rectifier has a total area of 40 × 9 mm2 and operates at different resonant frequencies, namely 3.5 GHz, 6 GHz, 8 GHz, 10 GHz and 18 GHz, with a good agreement between simulation and measurement. The maximum measured output DC voltage of the rectenna circuit is 600 mV with a maximum measured efficiency of 25% at 3.5 GHz, with an input power level of 0 dBm at a rectifier load of 300 Ω.
A Low-Phase-Noise 8 GHz Linear-Band Sub-Millimeter-Wave Phase-Locked Loop in 22 nm FD-SOI CMOS
Authors: Kebe Mamady , Sanduleanu Mihai
Published: May 2023

Low-phase noise and wideband phased-locked loops (PLLs) are crucial for high-data rate communication and imaging systems. Sub-millimeter-wave (sub-mm-wave) PLLs typically exhibit poor performance in terms of noise and bandwidth due to higher device parasitic capacitances, among other reasons. In this regard, a low-phase-noise, wideband, integer-N, type-II phase-locked loop was implemented in the 22 nm FD-SOI CMOS process. The proposed wideband linear differential tuning I/Q voltage-controlled oscillator (VCO) achieves an overall frequency range of 157.5–167.5 GHz with 8 GHz linear tuning and a phase noise of −113 dBc/Hz @ 100 KHz. Moreover, the fabricated PLL produces a phase noise less than −103 dBc/Hz @ 1 KHz and −128 dBc/Hz @ 100 KHz, corresponding to the lowest phase noise generated by a sub-millimeter-wave PLL to date. The measured RF output saturated power and DC power consumption of the PLL are 2 dBm and 120.75 mW, respectively, whereas the fabricated chip comprising a power amplifier and an integrated antenna occupies an area of 1.25 × 0.9 mm2.
Multiple Mechanisms Synergistically Induce Pseudomonas Aeruginosa Multiple Drug Resistance
Authors: Dai Pei , Jiao Fangyan , Yang Lulu , Bajinka Ousman , Abdelhalim Khalid A. , Wu Guojun , Tan Yurong
Published: May 2023

The aim of this study was to understand the molecular epidemiological characteristics and drug resistance mechanism of carbapenem-resistant Pseudomonas aeruginosa (CRPA) and to provide a theoretical basis for the prevention and treatment of CRPA infection in hospitals. A total of 34 CRPA strains were isolated, and resistance to 13 commonly used antibiotics was detected using the TDR-300B Plus VitEK-2 compact automatic bacterial identification instrument. Then, carbapenemase production was detected using the Carbe NP test. RT-qPCR was used to detect the expression of efflux pump MexA and outer membrane protein OprD, and PCR amplification and sequence analysis were used to detect class Ⅰ integrons carried by drug resistance genes. Our results showed that of the 34 CRPAs, 22 were multi-drug resistant (MDR), and five were extensively drug-resistant (XDR). Sequencing analysis showed that class Ⅰ integron mainly carried aminoglycosides or quinolones resistance genes. Multiple mechanisms play important roles in the formation and development of MDR or XDR.
Low-Temperature Microbiology Meets the Global Challenges of Our Time
Authors: Perfumo Amedea , Lo Giudice Angelina
Published: May 2023

Low-temperature microbiology is intimately associated with the exploration of the polar regions, and research in recent decades has focused on characterizing the microbial biodiversity of the cryosphere [...]
The Skin Microbiome: Current Techniques, Challenges, and Future Directions
Authors: Santiago-Rodriguez Tasha M. , Le François Brice , Macklaim Jean M. , Doukhanine Evgueni , Hollister Emily B.
Published: May 2023

Skin acts as a barrier that promotes the colonization of bacteria, fungi, archaea, and viruses whose membership and function may differ depending on the various specialized niches or micro-environments of the skin. The group of microorganisms inhabiting the skin, also known as the skin microbiome, offers protection against pathogens while actively interacting with the host’s immune system. Some members of the skin microbiome can also act as opportunistic pathogens. The skin microbiome is influenced by factors such as skin site, birth mode, genetics, environment, skin products, and skin conditions. The association(s) of the skin microbiome with health and disease has (have) been identified and characterized via culture-dependent and culture-independent methods. Culture-independent methods (such as high-throughput sequencing), in particular, have expanded our understanding of the skin microbiome’s role in maintaining health or promoting disease. However, the intrinsic challenges associated with the low microbial biomass and high host content of skin microbiome samples have hindered advancements in the field. In addition, the limitations of current collection and extraction methods and biases derived from sample preparation and analysis have significantly influenced the results and conclusions of many skin microbiome studies. Therefore, the present review discusses the technical challenges associated with the collection and processing of skin microbiome samples, the advantages and disadvantages of current sequencing approaches, and potential future areas of focus for the field.
Membrane Protein Bcest Is Involved in Hyphal Growth, Virulence and Stress Tolerance of Botrytis cinerea
Authors: Zhang Wei , Ge Bei-Bei , Lv Zhao-Yang , Park Kyung Seok , Shi Li-Ming , Zhang Ke-Cheng
Published: May 2023

Botrytis cinerea is a necrotrophic model fungal plant pathogen that causes grey mould, a devastating disease responsible for large losses in the agriculture sector. As important targets of fungicides, membrane proteins are hot spots in the research and development of fungicide products. We previously found that membrane protein Bcest may be closely related to the pathogenicity of Botrytis cinerea. Herein, we further explored its function. We generated and characterised ΔBcest deletion mutants of B. cinerea and constructed complemented strains. The ΔBcest deletion mutants exhibited reduced conidia germination and germ tube elongation. The functional activity of ΔBcest deletion mutants was investigated by reduced necrotic colonisation of B. cinerea on grapevine fruits and leaves. Targeted deletion of Bcest also blocked several phenotypic defects in aspects of mycelial growth, conidiation and virulence. All phenotypic defects were restored by targeted-gene complementation. The role of Bcest in pathogenicity was also supported by reverse-transcriptase real-time quantitative PCR results indicating that melanin synthesis gene Bcpks13 and virulence factor Bccdc14 were significantly downregulated in the early infection stage of the ΔBcest strain. Taken together, these results suggest that Bcest plays important roles in the regulation of various cellular processes in B. cinerea.
Two Bacillus spp. Strains Improve the Structure and Diversity of the Rhizosphere Soil Microbial Community of Lilium brownii var. viridulum
Authors: Tu Jing , Zhao Xin , Yang Yuanru , Yi Yongjian , Wang Hongying , Wei Baoyang , Zeng Liangbin
Published: May 2023

Lily Fusarium wilt disease caused by Fusarium spp. spreads rapidly and is highly destructive, leading to a severe reduction in yield. In this study, lily (Lilium brownii var. viridulum) bulbs were irrigated after planting with suspensions of two Bacillus strains that effectively control lily Fusarium wilt disease to assess their effects on the rhizosphere soil properties and microbial community. A high-throughput sequencing of microorganisms in the rhizosphere soil was performed and the soil physicochemical properties were measured. The FunGuild and Tax4Fun tools were used for a functional profile prediction. The results showed that Bacillus amyloliquefaciens BF1 and B. subtilis Y37 controlled lily Fusarium wilt disease with control efficacies of 58.74% and 68.93%, respectively, and effectively colonized the rhizosphere soil. BF1 and Y37 increased the bacterial diversity and richness of the rhizosphere soil and improved the physicochemical properties of the soil, thereby favoring the proliferation of beneficial microbes. The relative abundance of beneficial bacteria was increased and that of pathogenic bacteria was decreased. Bacillus abundance in the rhizosphere was positively correlated with most soil physicochemical properties, whereas Fusarium abundance was negatively correlated with most physicochemical properties. Functional prediction revealed that irrigation with BF1 and Y37 significantly upregulated glycolysis/gluconeogenesis among metabolism and absorption pathways. This study provides insights into the mechanism by which two Bacillus strains with antifungal activity, BF1 and Y37, antagonize plant pathogenic fungi and lays the foundation for their effective application as biocontrol agents.
The Effects of Coriandrum sativum L. and Cucurbita pepo L. against Gastrointestinal Parasites in Swine: An In Vivo Study
Authors: Băieş Mihai-Horia , Cotuţiu Vlad-Dan , Spînu Marina , Mathe Attila , Cozma-Petruț Anamaria , Miere Doina , Bolboacǎ Sorana D. , Cozma Vasile
Published: May 2023

Parasitic diseases are responsible for substantial losses in reproduction and productivity in swine, creating a major impairment to efficient and profitable livestock management. The use of phytotherapeutic remedies has notably increased over the past decade due to their bioavailability, decreased toxicity, non-polluting nature, and to some extent due to their antiparasitic effect. The aim of this study was to evaluate the antiparasitic potential of Cucurbita pepo L. and Coriandrum sativum L. against protozoa and nematodes found in swine. The samples were collected from weaners, fatteners, and sows and examined via flotation (Willis and McMaster), active sedimentation, Ziehl-Neelsen staining as modified by Henricksen, a modified Blagg method, and eggs/oocyst culture. The parasite species detected were Ascaris suum, Trichuris suis, Oesophagostomum spp., Balantioides coli (syn. Balantidium coli), Eimeria spp., and Cryptosporidium spp., depending on age category. A dose of 500 mg/kg bw/day of C. pepo and 170 mg/kg bw/day of C. sativum powders, administered for ten consecutive days, demonstrated a pronounced anthelmintic (pumpkin) and antiprotozoal (coriander) effect against the aforementioned parasites. Future studies are required to ascertain the optimal dose that maximizes their antiparasitic effectiveness. The current study represents the first Romanian report on the in vivo antiparasitic activity of these two plants tested on digestive parasites in swine.
Using Genomics to Decipher the Enigmatic Properties and Survival Adaptation of Candidate Phyla Radiation
Authors: Maatouk Mohamad , Rolain Jean-Marc , Bittar Fadi
Published: May 2023

Microbial ecology is a critical field for understanding the composition, diversity, and functions of microorganisms in various environmental and health-related processes. The discovery of Candidate Phyla Radiation (CPR) through culture-independent methods has introduced a new division of microbes characterized by a symbiotic/parasitic lifestyle, small cell size, and small genome. Despite being poorly understood, CPRs have garnered significant attention in recent years due to their widespread detection in a variety of environmental and clinical samples. These microorganisms have been found to exhibit a high degree of genetic diversity compared to other microbes. Several studies have shed light on their potential importance in global biogeochemical cycles and their impact on various human activities. In this review, we provide a systematic overview of the discovery of CPRs. We then focus on describing how the genomic characteristics of CPRs have helped them interact with and adapt to other microbes in different ecological niches. Future works should focus on discovering the metabolic capacities of CPRs and, if possible, isolating them to obtain a better understanding of these microorganisms.
Screening and Characterization of the Diversity of Food Microorganisms and Their Metabolites
Authors: Rocha João Miguel , Kovacevik Biljana , Veličkovska Sanja Kostadinović , Tamame Mercedes , Teixeira José António
Published: May 2023

Food is rarely kept in a sterile environment and the composition of microbial associations found in various foodstuffs is widely varied. Microorganisms in food usually originate from the natural microbiota of raw materials and the surrounding environments. Whether a species prevails depends upon its ability to adapt to intrinsic factors associated with foods, such as nutrient content; pH; water activity; oxidation–reduction potential; and antimicrobial properties, with various extrinsic factors playing a role, including temperature, relative humidity, atmosphere, and ambient pressure. Any change to these parameters may cause changes in the present microbial consortia. Therefore, it is important to identify which microbial consortia will thrive in particular foods and conditions. While active, microorganisms undergo many complex mechanisms that affect food quality and safety. Most beneficial food microorganisms belong to lactic acid bacteria and yeasts. Pathogenic and spoilage bacteria are usually Gram-negative, although there are some Gram-positive ones, such as Listeria monocytogenes, Clostridium botulinum, and C. perfringens. Some may merely cause spoilage, while others may be related to foodborne illnesses.
Blue and Yellow Light Induce Changes in Biochemical Composition and Ultrastructure of Limnospira fusiformis (Cyanoprokaryota)
Authors: Pelagatti Matilde , Mori Giovanna , Falsini Sara , Ballini Raffaello , Lazzara Luigi , Papini Alessio
Published: May 2023

Limnospira fusiformis (also known as Spirulina) is a cyanobacterium that is widely cultivated due to its economic importance. It has specific pigments such as phycocyanin that allow it to grow at different light wavelengths compared to other cultivated algae. Our study investigated the effect of yellow (590 nm) and blue (460 nm) light fields on various biochemical features, including the pigment concentration, protein content, dry weight, and cell ultrastructure of L. fusiformis. Our findings revealed that biomass growth was faster in yellow light compared to blue light, with a higher relative amount of proteins even after one day of exposure. However, after eight days, the relative protein content in yellow versus blue light was not statistically different. Furthermore, in yellow light, we observed a decrease in chlorophyll a, an increase in cyanophycin granules, and an increase in the amount of dilated thylakoids. On the other hand, in blue light, there was an increase in phycocyanin after one day, along with an increase in electron-dense bodies, which are attributable to carboxysomes. However, after eight days, the differences in pigment content compared to the control were not statistically significant. Our study showed that using specific wavelengths during the harvesting phase of spirulina growth can enhance phycocyanin content with blue light (after one day) and biomass, growth rates, and protein content with yellow light after six days. This highlights the biotechnological potential of this approach.
Authigenic Fe Mineralization in Shallow to Marginal Marine Environments: A Case Study from the Late Paleocene—Early Eocene Cambay Shale Formation
Authors: Roy Choudhury Tathagata , Singh Pragya , Chakraborty Arpita , Banerjee Santanu
Published: May 2023

The late Paleocene–early Eocene warm greenhouse conditions, characterized by elevated pCO2 levels in the atmosphere and a dramatic increase in sea surface temperature, prompted abundant authigenic glauconite formation within the shallow marine sediments worldwide by lowering the net sedimentation rate, increasing organic productivity and expanding the oxygen minimum zones to shallow oceans. The early Eocene marginal marine Cambay Shale Formation experienced episodes of marine inundation represented by limestone–green shale alternations. The shales host abundant authigenic light-green, dark-green, and brown pellets. A detailed characterization of the pellets of the Valia and Vastan lignite mines, integrating the sedimentological, petrographical, mineralogical, and mineral geochemical data, suggests two distinct varieties of Fe–silicate formation, viz. glauconite and chamosite. While the glauconitic green pellets are ubiquitous to Valia and Vastan mines, brown chamosite pellets are confined within the basal part of the green shale facies alternating with fossiliferous limestone in the Vastan mine. The glauconites of the Valia mine manifest a ‘nascent’ to ‘slightly evolved’ maturation stage of glauconitization, whereas the glauconites of the Vastan mine represent the ‘evolved’ type. The limestone–green shale alternation in the Valia mine is overlain by a ~4 m-thick spherulitic mudstone facies comprising monomineralic sideritic spherulites, reflecting a pure FeCO3 composition. The glauconites in the Cambay Shale Formation transformed from kaolinite-rich clay pellets under dys-oxic depositional conditions. The increasing anoxicity within the microenvironment, possibly amplified by the rapid oxidation of continent-derived organic matter, facilitated chamosite formation instead of glauconite. The increased freshwater influx into the marginal marine depositional environment resulted in immature, K-poor glauconites of the Valia mine. The formation of siderite spherulites overlying the limestone–green shale alternation relates to the beginning of the regressive phase of sedimentation.