bluuebunny/crossref_metadata_2025 huggingface dataset:
ANALISIS KECELAKAAN KERJA PADA STRUKTUR BAWAH BLENDING SILO PROYEK “EPC TALAVERA” TUBAN MENGGUNAKAN METODE BOWTIE
Authors: Bhayangkara Anugraha Hari , Setyawan Ary , Handayani Fajar Sri
Published: September 2023

The "EPC Talavera" Tuban Project is one of the major projects located in Tuban. Given that the construction of the Blending Silo is being built at a considerable height and certainly requires a large foundation structure, the construction of the Blending Silo carries a relatively high risk of workplace accidents. The purpose of this research is to identify workplace accident risks and evaluate the causes, impacts, and risk responses to accidents during the construction of the substructure of the Blending Silo in the "EPC Talavera" project. To determine the dominant workplace accident risks, risk assessment is performed using probability and severity calculations based on the Risk Management Standard AS/NZ 4360:1999, resulting in an Importance Index value. The method used to evaluate the causes, impacts, and risk responses to workplace accidents is the Bowtie Analysis Method. The results of this research indicate that the most dominant workplace accident risks are the risk of workers being pierced by sharp equipment, the risk of fingers getting caught in the bar bender machine, and the risk of workers being shocked/electrocuted by electrical currents. Based on the bowtie method, the most dominant causes of workplace accidents are scattered sharp equipment, careless/inattentive/unhealthy workers, inadequate equipment, poor bar bender machine conditions, bar bender machine operating methods, exposed welding tool cables, rainy/extreme weather conditions, and electrical current leaks in welding tool bodies. The most dominant impacts of workplace accidents are minor injuries, serious injuries/death, fires, and damage to the bar bender machine. Using the bowtie method, risk responses or controls for the most dominant workplace accident risks are also analyzed, along with an analysis of escalation factors and their controls.
Vibroacoustic heritage monitoring with a standalone system
Authors: Barone Fabrizio , Casazza Marco , Romano Rocco
Published: September 2023

This paper describes the results of the preliminary application tests for a stand-alone prototype data acquisition system. The system was developed for monitoring and characterizing the vibroacoustic landscape and, in particular, the vibroacoustic heritage, such as music instruments, performing spaces and areas of historical relevance. The tests, performed on a building in the historical center of Napoli, show a possible interconnection between acoustic and displacement signals, as external forcing of the monitored structure, with exchange of the cause-and-effect roles, especially at low frequency.
Back to the Past. The paleogeography as key to understand the Middle Palaeolithic peopling at Grotta dei Santi (Mt Argentario – Tuscany)
Authors: Conti Enrico , Arrighi Simona , Bigliazzi Niccolò , Boschin Francesco , Brindisi Albachiara , Brogi Kevin , Burgassi Allegra , Crezzini Jacopo , Dominici Clarissa , Latragna Lara , Luzzetti Tommaso , Marciani Giulia , Martini Ivan , Moroni Adriana , Pitzianti Sara , Ricci Stefano , Rossini Matteo , Salvi Alessandro , Scaramucci Sem , Spagnolo Vincenzo
Published: September 2023

The mobility of hunter-gatherer groups is crucial in understanding Palaeolithic settlement dynamics. The concept of mobility cannot be separated from the space in which it occurs, including landscape components, localization of critical resources and of other sites, and routes between them. Nevertheless, the landscape is not constant in time due to the geomorphological changes that occurred in the long timescale of Prehistory. Here we present a paleogeographic reconstruction of the coastal area around Grotta dei Santi during the Neandertal occupation. A GIS-based approach, combining geological, bathymetric, and sea-level fluctuations data, allows us to reconstruct the landscape around the cave at about 45 ky BP. The cave today opens onto a cliff facing the sea. The Neandertal occupation occurred with a sea-level 74 m lower than present-day. Consequently, the cave faced a vast coastal plain, playing a strategic role due to its position, allowing both proximity and control of essential resources.
Characterization and provenance of Roman tiles from the archaeological sites of Cariati, Scala Coeli and Terravecchia (Calabria, Southern Italy)
Authors: Taliano Grasso Armando , Medaglia Salvatore , De Luca Raffaella , Bloise Andrea , Carinola Sasha , Miriello Domenico
Published: September 2023

This paper concerns the archaeometric study carried out on a group of tiles and a brick belonging to the Roman period. In particular, the samples are dated back to the late 3rd-1st century BC and they come from the archaeological sites of Cariati, Scala Coeli and Terravecchia, in the province of Cosenza (Calabria, Southern Italy). Some of these samples are impressed with the stamps L./N. LUSIPETEL and M. MECONI, belonging to a production plant that operated in the north-central Ionian coast of the Calabria Region between the late Republican period and the Imperial age, owned to two important families of Petelia (today Strongoli). All samples were analyzed by Optical microscopy, X-ray Powder Diffraction and Energy Dispersion Microanalysis by Scanning Electron Microscope, in order to determine their minero-petrographic features and their geochemical composition, to identify the extraction area of the raw materials and the technological aspects related to the processing of the clay. In addition, the comparison between the chemical composition of the samples with the clay and the sand coming from some quarries of the Cariati area confirms that most of the samples were locally produced using raw materials from natural Pliocene outcrops.
Abundancia en poblaciones de peces cirujanos (Acanthuridae) en un ambiente insular del Caribe colombiano
Authors: Carvajal Gil John Alberto , Acero P. Arturo
Published: September 2023

En las comunidades coralinas, los peces herbívoros ocupan un papel principal en el mantenimiento y control de algas que son la base de la cadena trófica en los arrecifes coralinos. Entre los peces herbívoros de los arrecifes de coral, la familia Acanthuridae es de las más abundantes. Los efectos en los arrecifes de esta familia pueden variar en función de sus densidades, la estabilidad del ecosistema y sus interacciones, por lo que determinar el estado de sus poblaciones resulta de interés. En el archipiélago de San Andrés, Providencia y Santa Catalina (ASAP) falta información del estado actual de las abundancias y densidades de este grupo. Por lo tanto, mediante censos visuales de especies del género Acanthurus se estimaron los estados poblacionales en comunidades coralinas del ASAP y las diferencias poblacionales entre los sitios evaluados, lo cual evidenció un total de 6075 individuos, además de una relación positiva de abundancias en las islas de San Andrés y Providencia con respecto a las islas cayos Bolívar y Serrana. En cuanto a la preferencia de hábitat de juveniles se encontró una especialización y preferencia de hábitat de A. coeruleus por las zonas arrecifales, entretanto A. tractus mostró preferencia por los manglares.
Registros de escarabajos coprófagos (Coleoptera: Scarabaeidae) depositados en la Colección Entomológica de la Orinoquia (CEO)
Authors: Agudelo Martínez Juan Carlos , Pérez-Buitrago Néstor , Lopera Alejandro
Published: September 2023

Analizamos 3371 especímenes del departamento de Arauca y Casanare en la Orinoquia colombiana depositados en la Colección Entomológica de la Orinoquia (CEO). Los especímenes corresponden a 20 géneros, 34 especies y 16 morfoespecies de escarabajos coprófagos. Este conjunto de datos reporta el 30,6 % de los registros disponibles para este grupo taxonómico en la Orinoquia colombiana (11025 especímenes) en el GBIF, reportando el incremento en el rango altitudinal de la especie Genieridium bordoni (Martínez, 1992) y de Dichotomius costaricensis (Luederwalt, 1935). Así mismo, amplía el rango de distribución para 34 de las especies, contribuyendo al conocimiento actual de las distribuciones geográficas de Scarabaeidae en Colombia.
Morfología y biometría de racimos, frutos y semillas de Attalea bassleriana en Alto Amazonas, Perú
Authors: Rodríguez-Cabrera Edward Hanz , Moraes R. Mónica , Rojas-Fox Jenny , Grández Ríos César , Mejía Carhuanca Kember Mateo , Rodríguez del Castillo Ángel Martín
Published: September 2023

Se evaluó la morfología y biometría de racimos, frutos y semillas de Attalea bassleriana en las localidades de Paraíso, Libertad de Cuiparillo y Santa Lucía en Alto Amazonas, Perú, para comprender su variabilidad interpoblacional y contribuir al esclarecimiento taxonómico de la especie. Se describió el ambiente donde se desarrolla la palmera mediante 70 descriptores que fueron comparados con ANOVA y Kruskal-Wallis, correlacionados a través Spearman y visualizados gráficamente mediante el Análisis de Componentes Principales (PCA). Para la descripción del ambiente se utilizó una ficha de toma de datos para muestras de campo. En total, 23 descriptores biométricos presentaron diferencias significativas (p < 0,05). Las correlaciones más altas fueron número de frutos/peso de racimo (0,921) y peso del fruto/diámetro del fruto (0,844). El PCA demuestra la variabilidad de los frutos entre las poblaciones y denota a Paraíso como la menos variable y más diferenciada. Asimismo, se observó que la especie se encuentra en bosques de terraza, bosques pantanosos, tierras agrícolas y ganaderas, entre 145 y 159 m s. n. m. Esta información evidencia la identificación taxonómica del shebón y es un referente tanto para el aprovechamiento adecuado de frutos y semillas como para su aplicación en el mejoramiento genético.
Dípteros asociados a fitotelmata en un bosque pluvial tropical (bp-T) en Chocó, Colombia
Authors: Wolff Martha Isabel , Torres-Toro Juliana , Henao-Sepúlveda Carolina , Pérez Sandra , Montoya Augusto , Betancur Julio
Published: September 2023

Presentamos 7418 registros de insectos del orden Diptera, correspondientes a 7569 ejemplares depositados en la Colección Entomológica de la Universidad de Antioquia (CEUA), los cuales incluyen información asociada a los taxones vegetales en donde se albergaban. Los ejemplares fueron recolectados en/o criados a partir de pequeños cuerpos de agua acumulados en algunas partes de plantas fitotelmas en un bosque pluvial tropical del departamento del Chocó. El conjunto de datos es de acceso público a través de Sistema de Información sobre Biodiversidad de Colombia (SiB-Colombia).
Low size, weight, power, and cost (SWaP-C) payload for autonomous navigation and mapping on an unmanned ground vehicle
Authors: Ennasr Osama , Dodd Brandon , Paquette Michael , Ellison Charles , Glaspell Garry
Published: September 2023

Autonomous navigation and unknown environment exploration with an unmanned ground vehicle (UGV) is extremely challenging. This report investigates a mapping and exploration solution utilizing low size, weight, power, and cost payloads. The platform presented here leverages simultaneous localization and mapping to efficiently explore unknown areas by finding navigable routes. The solution utilizes a diverse sensor payload that includes wheel encoders, 3D lidar, and red-green-blue and depth cameras. The main goal of this effort is to leverage path planning and navigation for mapping and exploration with a UGV to produce an accurate 3D map. The solution provided also leverages the Robot Operating System.
Sentiment Analysis of Tourist Attraction Review from TripAdvisor Using CNN and LSTM
Authors: Adrian Manurung Kevin
Published: September 2023

The tourism sector has an important role in driving the economy. To find out the positive or negative responses of tourists, one of them is grouping through sentiment analysis using deep learning. The data used the tourist attraction dataset from TripAdvisor from several categories such as water and amusement park, nature, and museum. The methods used in this research are convolutional neural network (CNN) and long short-term memory (LSTM). In addition, Word2vec for feature extraction and Synthetic Minority Over-sampling (SMOTE) for handling imbalanced datasets will be used for this research. There are several scenarios used to perform sentiment analysis, with early stopping and with hyperparameter tuning using random search. The highest performance obtained on water and amusement park, nature, and museum category data is 83%, 97%, and 88% respectively for accuracy and 91%, 92%, and 93% respectively for F1-score. For the use of sentiment analysis methods, CNN can perform with the highest F1-score and LSTM can perform with the highest accuracy.
Evaluation of Bone Quality in Patients With Bruxism
Authors: Kotanli Sedef , DOĞAN Mehmet Sinan , Ozturk Elif Meltem Aslan , dogan mehmet emin , Uluisik Nurbanu
Published: September 2023

Aim: The aim of this study is to evaluate the mandibular bone quality using radiomorphometric indices and Fractal dimension [FD] analysis in orthopantomography [OPG].
Material and Methods: OPGs of 100 patients, 50 bruxers and 50 non-bruxers, were included in this retrospective study. Values ​​such as mental index [MI], panoramic mandibular index [PMI], antegonial notch depth [AND], gonial index [GI], antegonial index [AI] and mandibular cortical index [MCI] were calculated in OPG. Eight bilateral areas of interest [ROI] were selected on ort for FD analysis: ROI 1, mandibular condyle; ROI 2, mandibular ramus; ROI 3, mandibular angulus; ROI 4, mandibular mental area.
Results: MI, PMI and AND values ​​were higher in bruxers than in the control group [p<0.05]. There is no statistically significant difference between AI and MCI measured on the right and left sides between bruxism patients and control group patients [p>0.05]. As a result of the calculations, the FD values ​​of the left condyle [p=0.02] and left angulus [p=0.03] areas showed a statistically significant difference between individuals with and without bruxer. No statistically significant difference was found in the FD measurements calculated from the ramus and mental areas on the right and left sides [p>0.05]. The relationship between FD values ​​and gender in these areas was examined and no statistically significant difference was found [p<0.05].
Conclusions: MI, PMI and AND measurements can be used in the diagnosis or follow-up of bruxism. There is no difference in mandibular cortical bone height in patients with and without bruxism according to AI and MCI. The mean GI measured on the right side differed between groups. FD values ​​of the mandibular trabecular bone are affected by bruxism in the right condyle and right angulus areas.
The Decision-making Pathway and Enablers to the Utilization of Assisted Reproductive Technology in Lagos Metropolis: an interpretive phenomenological study
Authors: Ajike Saratu Omagbemi , Asiegbu Ugochinyere Chizurumuoke , Lawal Saheed Akinmayowa , Nantamu Simon
Published: September 2023

Background
Women experiencing infertility face barriers to access Assisted Reproductive Technology (ART) services in Nigeria. Despite these barriers, some still access these services. This study explored the decision-making pathway and enablers of the use of ARTs among such persons in Lagos.
Methods
Using an interpretive phenomenological approach, we explored the decision-making process and enablers motivating the use of ART services among women who have experienced infertility. An open-ended structured in-depth interview guide was used to collect data that were analyzed thematically.
Results
Findings revealed that the decision-making pathway involved personal, interpersonal or relational thought processes to use Assisted Reproductive Technology for assisted conception and enablers which were a mix of emotional support from the mother, spouses, social pressures, information about health condition, professional and lay suggestions all contributed to the eventual use of ARTs.
Conclusion
Results of this study indicate that infertile women received different forms of support that enabled the use of ARTs. However, women who used ART services experienced several psychosocial challenges. These ARTs have given infertile and childless couples some ray of hope and the chance to escape the burden and social stigma of childlessness. This study recommends that public health professionals collaborate with ART service providers in providing psychosocial care in addition to basic clinical care. Future studies can explore the perspectives of significant others as they played a key role in the decision and motivated ART use.
Investigations on the influence of angular and linear misalignment on the fatigue strength of HFMI-treated structural steel butt joints
Authors: Ripsch Benjamin , Gabriel Göran , Gericke Andreas , Henkel Knuth-Michael
Published: September 2023

The effect of High Frequency Metal Impact (HFMI) treatment on the fatigue resistance of structural steel S355 butt welds with macro geometric imperfections outside of the usual fatigue design limits was investigated. Thin-walled specimens were manufactured with different degrees of angular and linear misalignment and fatigue tested in the as welded and HFMI treated condition. The fatigue test results show that HFMI treatment can compensate the fatigue-reducing effects of macro geometric imperfections, but also that linear and axial misalignment do not reduce the fatigue strength of butt welds as severely as rules and guidelines suggest. Numerical analysis shows that the application of the notch stress approach according to current standards and guidelines delivers conservative assessment results, which might not always be representative for the actual fatigue strength benefit that can be gained by HFMI treatment.
Comparison of Low-Grade Gliomas Molecular Features and Survival by 1p/19q Codeletion in The Cancer Genome Atlas
Authors: Ma Jun , Su Xi , Tang Mingjun , Sa Yu , Huang Xian , Zhang Ning
Published: September 2023

In patients with low-grade gliomas (LGG), the prognosis is significantly favorable in those with 1p/19q codeletion than in those with 1p/19q intact. Although 1p/19q codeletion has emerged as an accepted indicator for molecular typing of gliomas, numerous studies point to the need to further investigate the overall transcriptomic molecular changes associated with it. To explore the genome-wide effects of 1p/19q codeletion, we evaluated multiple omics profiles from The Cancer Genome Atlas LGG cohort. After systematic analysis, we identified a modest number of genomic features, including gene expression (n = 14), protein expression (n = 8), DNA methylation (n = 9), somatic mutation (n = 7) and copy number variation (n = 35). These features were highly corelated with 1p/19q codeletion status of patients. These features are then used to construct support vector machine classifiers and identify survival-related markers. It is helpful from this research to generate fresh insights into the alterations occurring behind the 1p/19q codeletion and to elucidate the mechanisms of LGG histological typing.
Development of a Precision Medicine Competency Framework for the Therapeutics Industry
Authors: Conway Nicholette , Chisholm Orin
Published: September 2023

INTRODUCTION: The successful adoption of precision medicine relies on the development of effective treatments and judicious utilization within a supportive healthcare system. Embracing a learning healthcare capability will be crucial to navigating the disruptions arising from rapid scientific and technological innovation.
AIM: to build a Precision Medicine (PM) competency framework that can be used across the Medical Technology and Pharmaceutical (MTP) industries to build a confident and capable workforce, support cross-disciplinary work and collaboration, and instil a continuous learning mindset.
METHODOLOGY: A desktop research review of current literature, curriculum, and healthcare trends identified a core set of domains and subdomains related to precision medicine competencies. A survey was distributed to the Industry Genomics Network Alliance (InGENA) members in 2021 to confirm the relevance and applicability of the domains and subdomains to their current work practice and their expected work practice in 5 years’ time.
RESULTS: Four domains were identified: medical science and technology; translational and clinical application; governance and regulation and professional practice. Each domain has a series of subdomains and patient needs were integrated across all four of the domains. Survey results confirmed the applicability of these domains to the MTP industry.
CONCLUSION: The Framework was well accepted by industry, with a strong interest from related disciplines including allied health professionals. Given the pace of change this framework will need regular review and updating. The principles can be used to define frameworks for other technologies such as Cell and Gene Technologies (CGT) and Regenerative Medicines (RM).
Investigation on micro milling of cemented carbide with ball nose and corner radius diamond coated end mills
Authors: Figueiredo Daniel Amaral , Silva Joana , Silva Tiago , Jesus Abílio , Fernandes Cristina , Davim Paulo
Published: September 2023

Micro milling of cemented carbides is a challenging task due to their high hardness, low toughness and high wear resistance. Ensuring good surface quality and dimensional accuracy is crucial for extending parts service life, which in turn enhances economical and environmental sustainability. This paper is mainly focused on evaluating surface formation mechanisms, scale effects, fracture behaviour and chip formation using distinct cemented carbide micro milling tools with multi layer diamond HF-CVD. In order to achieve higher precision and more efficient micro milling operations on WC-15Co and WC-10Co, a systematic experimental approach has been carried out. The influence of cutting parameters, achievable surface quality and defects occurrence were thoroughly examined. Experimental results evidence the influence of operational conditions on the chip formation of cemented carbides as well as an important impact of the utilized cutting tool. Micro pits, cracks, thin ploughing layer and fractured workpiece edges are amongst the observed surface damage mechanisms. A ductile cutting regime of the high-hardness composite material is confirmed, exhibited by the plastic deformation even when small depths of cut are considered.
NOL3 induced feedback loop between RCC cells and TAMs promotes TKI resistance and unfavorable prognosis in renal cancer
Authors: Cui Xingang , Wang Hongru , Tian Yijun , Lv Jianmin , Shi Xiaokai , Sun Ye , Qian Cheng , Song Xu , Gan Sishun
Published: September 2023

TKI resistance of advanced ccRCC patients usually leads to poor prognosis. Interaction between tumor cells and tumor-associated macrophages (TAMs) has been reported to facilitate tumor progression. However, the underlying mechanism remains unclear. This study employed cell functional experiments, IHC, ELISA, and subcutaneous tumor formation models to explore the interaction between ccRCC and TAMs. We found that the expression of NOL3 was upregulated in ccRCC using the online database. The NOL3 over-expressing ccRCC cell lines ACHN and 786-O showed enhanced proliferation and anti-apoptosis ability. In addition, THP1-derived macrophages co-cultured with ccRCC cells exhibited an increased trend towards M2-like polarization. TAMs could stimulate ccRCC to secret more VEGF, which promotes TKI resistance. ccRCC patients from two independent cohorts were screened to investigate the prediction accuracy of NOL3 and the relationship between NOL3 expression and TAMs infiltration. IHC staining quantified by H-score revealed a negative correlation between NOL3 expression and disease progression and a positive correlation with TAMs infiltration. Moreover, NOL3, CD163, and TNM Stage were also found to be the independent risk factors for predicting the outcome of ccRCC patients. Integrating NOL3, TAMs and TNM Stage could predict the prognosis of ccRCC with better accuracy. Overexpressing NOL3 enhanced the infiltration of TAMs and VEGF secretion in mouse tumors, while depletion of macrophages enhanced TKI efficacy. In summary, a positive feedback loop between ccRCC cells and TAMs induced by NOL3 promotes TKI resistance, and targeting NOL3/TAMs might be a potential strategy to alleviate TKI resistance.
Pathogenic variants in SMARCA1 cause an X-linked neurodevelopmental disorder modulated by NURF complex composition
Authors: Picketts David , Mirzaa Ghayda , Yan Keqin , Relator Raissa , Timpano Sara , Yalcin Binnaz , Collins Stephan , Ziegler Alban , Pao Emily , Oyama Nora , Brischoux-Boucher Elise , PIARD Juliette , Monaghan Kristin , Sacoto Maria Guillen , Dobyns William , Park Kristen , Fernández-Mayoralas Daniel , Fernández-Jaén Alberto , Jayakar Parul , Brusco Alfredo , Antona Vincenzo , Giorgio Elisa , Kvarnung Malin , Isidor Bertrand , Conrad Solène , Cogné Benjamin , Deb Wallid , Stuurman K.E. , Sterbova Katalin , Smal Noor , Weckhuysen Sarah , Oegema Renske , Innes Micheil , Latsko Maeson , Ben-Omran Tawfeg , Yeh Rebecca , Kruer Michael , Bakhtiari Somayeh , Papavasiliou Antigone , Moutton Sébastien , Nambot Sophie , Chanprasert Sirisak , Paolucci Sarah , Miller Kait , Burton Barbara , Kim Katherine , O'Heir Emily , Bruwer Zandre , Donald Kirsten , Kleefstra Tjitske , Goldstein Amy , Angle Brad , Bontempo Kelly , Miny Peter , Joset Pascal , Demurger Florence , Hobson Emma , Pang Lewis , Carpenter Lori , Li Dong , Bonneau Dominique , Sadikovic Bekim
Published: September 2023

Pathogenic variants in ATP-dependent chromatin remodeling proteins are a recurrent cause of neurodevelopmental disorders (NDDs). The NURF complex consists of BPTF and either the SNF2H (SMARCA5) or SNF2L (SMARCA1) ISWI-chromatin remodeling enzyme. Pathogenic variants in BPTF and SMARCA5 were previously implicated in NDDs. Here, we describe 40 individuals from 30 families with de novo or maternally inherited pathogenic variants in SMARCA1. This novel NDD was associated with mild to severe ID/DD, delayed or regressive speech development, and some recurrent facial dysmorphisms. Individuals carrying SMARCA1 loss-of-function variants exhibited a mild genome-wide DNA methylation profile and a high penetrance of macrocephaly. Genetic dissection of the NURF complex using Smarca1, Smarca5, and Bptfsingle and double mouse knockouts revealed the importance of NURF composition and dosage for proper forebrain development. Finally, we propose that genetic alterations affecting different NURF components result in a NDD with a broad clinical spectrum.
Debonding Stresses of Metal Orthodontic Brackets Retained to Tooth Enamel with Co-Cured Self-Etch Primer
Authors: Abdelaziz Khalid M. , Alshahrani Abdulaziz M. , Algarni Youssef A. , Alayyash Afnan
Published: September 2023

Background: Applying the recently suggested resin primer co-curing technique could save some of patients’ and orthodontists’ time. This study evaluated the efficiency of self-etch primer co-curing protocol in retaining orthodontic brackets.
Methods: A self-etch orthodontic resin primer was pre-cured or co-cured together with the resin adhesive (Groups 1 and 2) to retain either non-coated or pre-coated orthodontic brackets (Subgroups 1 and 2) on the buccal surfaces of extracted premolars. The debonding stress values were evaluated immediately after bonding (category 1) and after exposure to cyclic intraoral hydraulic, thermal, and mechanical stress simulations (Category 2). The adhesive remanent index (ARI) was also assessed for each test category.
Results: The co-cured primer provided higher immediate debonding stress values of both non- and pre-coated brackets (Tukey’s, P<0.05) than did the pre-cured primer. The delayed debonding stress values were lower than the immediately assessed values (Tukey’s, P<0.05) for both types of brackets. No difference (Mann Whitney’s, P>0.05) was noticed in the ARIs of all test categories.
Conclusions: Although the intraoral stress simulations reduce the brackets’ debonding stress values, co-curing the self-etch orthodontic primer can offer clinically efficient bonding of both non- and pre-coated metal brackets with no harm to the tooth enamel on debonding.
Chromosome abnormalities and copy number variations in fetuses with ultrasound soft markers: a retrospective study
Authors: Zhang Hao , Chen Quan , Li Xue , Chen Huijuan , Liu Lin , Zhou Shijie , Xu Zhihong
Published: September 2023

Purpose
Although previous results revealed that genetic aberrations were associated with ultrasound soft markers, the detection rates of chromosomal aberrations and P/LP CNVs varied among different studies. Thus, the detection of chromosome abnormalities and copy number variations (CNVs) in fetuses with ultrasound soft markers were investigated in our study.
Methods
A total of 2422 fetuses, including 1005 fetuses with ultrasound soft markers and 1417 fetuses without ultrasound soft markers, were included in our study. CNV-seq, combined with karyotyping or QF-PCR, was performed to detect chromosome abnormalities and CNVs. Statistical analysis was performed using SPSS 19.0.
Results
Our study detected 28 and 22 chromosome abnormalities in fetuses with and without ultrasound soft markers, respectively. Meanwhile, 24 and 10 P/LP CNVs were detected in fetuses with and without ultrasound soft markers, respectively. These results revealed that the detection rates of chromosome abnormalities and P/LP CNVs were significantly increased in fetuses with ultrasound soft markers. Subsequent analysis unveiled that the detection rates of chromosome abnormalities and P/LP CNVs varied in fetuses with different ultrasound soft markers. The detection rates of chromosome abnormalities in fetuses with thickened NT elevated significantly, while P/LP CNVs were more likely detected in fetuses with renal abnormalities. Additionally, our results showed that the detection rates of chromosome abnormalities were positively correlated with NT thickness in fetuses with thickened NT.
Conclusion
Our results revealed that fetuses with ultrasound soft markers had a higher risk of chromosome abnormalities and P/LP CNVs.
Establishment and Validation of a Predictive Model for the Risk of Invasive Mechanical Ventilation in Elderly Patients with Sepsis
Authors: Zhu Simeng
Published: September 2023

Background
The aim of the research was to discover risk elements and create a useful nomogram for predicting the occurrence of invasive mechanical ventilation (IMV) in elderly patients with sepsis.
Methods
Sepsis patients who were elderly, aged 65 years or older, were selected from the Medical Information Mart for Intensive Care (MIMIC)-IV database. Demographic and laboratory test information were collected on the first day of Intensive Care Unit (ICU) admission. Participants were 7:3 randomly assigned divisions into training and validation sets. The features of training set were used to determine risk factors for predicting invasive mechanical ventilation. The least absolute shrinkage and selection operator (LASSO) regression was employed to recognize predictors. Subsequently, the training set was utilized to create a nomogram. The validity of the nomogram was evaluated using receiver operating characteristic (ROC) analysis, decision curve analysis (DCA), and calibration curve analysis.
Results
We enrolled 7,868 patients, with 5,259 (66.8%) receiving invasive mechanical ventilation. In the IMV group, hospital mortality was higher than in the non-IMV group (23% vs. 13%, p < 0.001). Logistic regression analysis identified SpO2, hemoglobin, anion gap, chloride, vasopressor drugs, and ICU length of stay (LOS) as predictors, which were integrated into a nomogram. The AUC of the nomogram was 0.84 in both training set and validation set. The calibration plot demonstrated that the nomogram effectively predicted the requirement for IMV in both datasets. DCA proved the clinical values of the nomogram.
Conclusion
The nomogram provides a predictive tool for identifying the demand for invasive mechanical ventilation in sepsis patients who aged 65 or older. This model can aid healthcare professionals in identifying high-risk patients earlier and implementing timely interventions to improve their prognosis.
Rise of oligodendroglioma hypermutator phenotype from a subclone harboring TP53 mutation after TMZ treatment
Authors: Higuchi Fumi , Uzuka Takeo , Matsuda Hadzki , Sumi Takuma , Iwata Kayoko , Namatame Takashi , Shin Masahiro , Akutsu Hiroyoshi , Ueki Keisuke
Published: September 2023

Oligodendrogliomas characterized and defined by 1p/19q co-deletion are slowly glowing tumors showing better prognosis than astrocytomas. TP53 mutation is rare in oligodendrogliomas while the vast majority of astrocytomas harbor the mutation, making TP53 mutation mutually exclusive with 1p/19q codeletion in lower grade gliomas virtually. We report a case of 51-year-old women with a left fronto-temporal oligodendroglioma that contained a small portion with a TP53 mutation, R248Q, at the initial surgery. On a first, slow-growing recurrence 29 months after radiation and nitrosoures-based chemotherapy, the patient underwent TMZ chemotherapy. The recurrent tumor responded well to TMZ, but developed a rapid progression after 6 cycles as a malignant hypermutator tumor with a MSH6 mutation. Most of the recurrent tumor lacked typical oligodendroglioma morphology that was observed in the primary tumor, while it retained the IDH1 mutation and 1p/19q co-deletion. The identical TP53 mutation observed in the small portion of the primary tumor was universal in the recurrence. This case embodied the theoretically understandable clonal expansion of the TP53 mutation with additional mismatch repair gene dysfunction leading to hypermutator phenotype, and thus indicated that TP53 mutation in oligodendroglioma, although not common, may play a critical role in the development of hypermutator after TMZ treatment.
MiR-146a-5p plays a therapeutic role in temporomandibular joint osteoarthritis in rats: an in vivo study
Authors: Jingfen Cun , Congchong Shi , Yong Chen , Li Zhang , Zhi Zhou , Yu Ding
Published: September 2023

Methods
TMJ-OA disease model was established by combined injection of IL-1β and TNF-α, and miR-146a-5p agomir was injected into the rat’s joint cavity, then, observe the changes of temporomandibular joint tissue structure and related proteins by HE, safranine O-fast green, immunohistochemical staining.
Results
The inflammatory secretions were significantly reduced in the experimental group (injection of miR-146a-5p agomir), the structure of cartilage layers was clearer than that of the control group, and the expression of MMP9 and COX2 decreased compared with the control group, while the expression of type II collagen increased.
Conclusion
MiR-146a-5p, as a "protective factor", it has the potential to treat osteoarthritis, attenuates the inflammatory response of osteoarthritis and moderates the progression of osteoarthritis through its apparent anti-inflammatory ability.
Joint Application of Multiple Inflammatory Cytokines in Development of Heart Failure
Authors: Yu Mingyan , Zhang Qingquan , Zhu Jiaoyan , Xu Louyuan , Chen Liang , Zheng Koulong
Published: September 2023

Background
Heart failure is a serious heart ailment that affects millions of people globally in terms of their quality of life and general health. In the development and pathophysiology of heart failure, cytokines play a pivotal role. In-depth comprehension of the role cytokines assume in heart failure holds the potential to offer more precise grounds for early diagnosis, therapeutic interventions, and prognostic assessments of this cardiac ailment.
Purpose
This article aimed to explore the correlation between inflammation-related cytokines and heart failure.
Methods
The study recruited a total of 116 participants who were subsequently divided into a heart failure group (n = 59) and a non-heart failure group (n = 57). Laboratory tests were conducted for various inflammatory markers, and the level of each marker was compared between the heart failure group and the healthy control group (n = 57) matched for age and gender to determine the correlation with heart failure. Compared with patients in the non-heart failure group, significantly higher levels of inflammatory response markers (hs-CRP, ESR) and cytokines (IL-6, IL-8, IL-10, IL-17, TNF-α), and decreased levels of albumin, complement C3, and phospholipase A2 were noted in heart failure patients.
Results
Through logistic regression analysis, it has been elucidated that the cytokines IL-6(OR = 1.269,95% CI:1.049–1.472,P = 0.002), IL-8(OR = 1.071,95% CI:1.012–1.134,P = 0.018), and IL-17(OR = 1.180,95% CI:1.010–1.378,P = 0.037) act as autonomous risk factors in the development of heart failure, while Lp-PLA2(OR = 0.986,95% CI:0.972–0.999,P = 0.036) and albumin(OR = 0.476,95% CI:0.267–0.848,P = 0.012) exhibit a protective role against this condition.According to the analysis of ROC curves: the combined use of multiple cytokines(AUC = 0.9277,95% CI:0.8788–0.9767, P < 0.0001,YI = 0.7779) demonstrates higher efficacy in diagnosing heart failure, yet NTpro-BNP(AUC = 0.9914,95% CI:0.9805-1.000, P < 0.0001,YI = 0.9914) remains the clinical preference. In the context of systemic inflammation, the combined diagnostic approach of multiple cytokines with hsCRP(AUC = 0.9358,95% CI:0.8919–0.9797, P < 0.0001,YI = 0.7942) exhibits greater sensitivity and specificity.
Body composition assessment by Artificial Intelligence can be a predictive tool for short-term postoperative complications in Hartmann’s reversals
Authors: Suthakaran Reshi , Cao Ke , Arafat Yasser , Yeung Josephine , Chan Steven , Master Mobin , Faragher Ian G. , Baird Paul N. , Yeung Justin M.C
Published: September 2023

Background
Hartmann’s reversal is a complex elective procedure that requires careful planning and patient optimisation to help reduce postoperative complications. Preoperative evaluation of body composition has been useful in identifying patients at high risk of short-term postoperative outcomes following colorectal cancer surgery. We sought to explore the use of our in-house derived Artificial Intelligence (AI) algorithm to measure body composition within patients undergoing Hartmann’s reversal procedure in the prediction of short-term postoperative complications.
Methods
A retrospective study of all patients who underwent Hartmann's reversal within a single tertiary referral centre (Western) in Melbourne, Australia and who had a preoperative Computerised Tomography (CT) scan performed. Body composition was measured using our previously validated AI algorithm for body segmentation developed by the Department of Surgery, Western Precinct, University of Melbourne.
Results
Between 2010 and 2020, 47 patients (mean age 63.1 ± 12.3 years; male, n = 28 (59.6%)underwent body composition analysis. Twenty-one patients (44.7%) were sarcopenic, and 12 (25.5%) had evidence of sarcopenic obesity. The most common postoperative complication was surgical site infection (SSI) (n = 8, 17%). Sarcopenia (n = 7, 87.5%, p = 0.02) and sarcopenic obesity (n = 5, 62.5%, p = 0.02) were significantly associated with SSIs. The risks of developing an SSI were 8.7 times greater when sarcopenia was present.
Conclusion
Sarcopenia and sarcopenic obesity were related to postoperative complications following Hartmann’s reversal. Body composition measured by a validated AI algorithm may be a beneficial tool for predicting short-term surgical outcomes for these patients.
Use of body weight-adjusted FEV1/FVC as an index to predict the risk of sarcopenia among elderly people in the community
Authors: Wang Cai-xia , Zhu Gui-qi , Meng Qiong , Lyu Xiao-yu , lou Ling-zhi , Liu Jian-zhong , Xu Xue-fan , Jiang Hai-xian , Peng Lu-lu , Fu Li-li , Lyu Jie-wen , Bai Chun , Mo Bao-qing , Tao Hai-qi , Yu Yang
Published: September 2023

Background
The quality and function of skeletal muscle decrease with aging in humans. Meanwhile, sarcopenia leads to increased fat mass and decreased muscle mass in the elderly. Furthermore, sarcopenia results in decreased respiratory muscle mass and strength in the elderly, as well as reduced respiratory function. Sarcopenia is an important cause of death from aspiration pneumonia, chronic obstructive pulmonary disease, lung cancer, and other respiratory diseases in the elderly. Therefore, it is of great significance to develop an inexpensive and simple respiratory function test to identify elderly patients at risk of sarcopenia.
Methods
In this cross-sectional survey, subjects who participated in the physical examination project of the second Community Health Service Center of Pengpu Town, Jing’an District, Shanghai, China were eligible. From April to September 2021, 1257 elderly people underwent a health screening. Demographic data were collected via self-completed forms. Physical data such as height, weight, and disease history were collected via consultation and physical examination by clinicians. Medical examination data included the results of bioelectrical impedance analysis, respiratory function tests, chest X-ray, and electrocardiogram. Statistical analysis was performed using Student’s t-test, the chi-squared test, Spearman’s correlation analysis, and receiver operating characteristic (ROC) curve analysis.
Results
In total, 663 subjects were enrolled in this study, including 285 women (70.154 ± 3.931 years old) and 207 men (70.437 ± 3.891 years old) without sarcopenia and 101 women (71.237 ± 4.858 years old) and 70 men (72.12 ± 5.232 years old) with sarcopenia. Age and body mass index significantly differed between subjects with and without sarcopenia. In addition, combined forced vital capacity (FVC), forced expiratory volume in 1 s (FEV1), FEV1/FVC, maximum inspiratory pressure (MIP), and peak inspiratory flow (PIF) adjusted for body weight significantly differed between subjects with and without sarcopenia for both men and women. The sensitivity and specificity of body weight-adjusted FEV1/FVC for predicting the risk of sarcopenia in elderly subjects were high. For body weight-adjusted FEV1/FVC in men, the area under the ROC curve, sensitivity, specificity, and cutoff were 0.816, 0.829, 0.676, and 0.012, respectively, whereas the values were 0.830, 0.782, 0.785, and 0.015, respectively, in women.
Conclusions
The results in our study clearly indicate that body weight-adjusted FEV1/FVC was positivity correlated with the risk of sarcopenia in elderly men and women.
Dual-Targeted Nano-Encapsulation of Neonatal Porcine Islet-Like Cell Clusters with Triiodothyronine-Loaded Bifunctional Polymersomes
Authors: Lee Sang Hoon , Kim Minse , Lee Eun Jin , Ahn Sun Mi , Ahn Yu-Rim , Choi Jaewon , Kang Jung-Taek , Kim Hyun-Ouk
Published: September 2023

There is growing evidence that neonatal porcine islet-like cell clusters (NPCCs) isolated from piglets can be used to treat type 1 diabetes in humans. However, graft rejection is a common complication in humans owing to the prevalence of xenoantigens in porcine. Therefore, researchers have investigated various islet encapsulation techniques that could protect against these antigens. To this end, this study presents a robust nano-encapsulation method based on bifunctional polymersomes (PSomes), in which N-hydroxysuccinimide (NHS) and maleimide (Mal) groups conjugated to the PSomes terminal interact with the amine and thiol groups on the surface of NPCCs to induce dual targeting via two covalent bonds. The findings indicate that the ratio of NHS to Mal on PSomes is optimal for dual targeting. Moreover, triiodothyronine (T3) is known to promotes pancreatic islet maturation and differentiation of endocrine cells into beta cells. T3 encapsulated in PSomes is shown to increase the glucose sensitivity of NPCCs and enhance insulin secretion from NPCCs. Furthermore, improvements in the nano-encapsulation efficiency and insulin-secreting capability of NPCCs through dual targeting via dual-Psomes are demonstrated. In conclusion, the proposed nano-encapsulation technique could pave the way for significant advances in islet nano-encapsulation and the imprevement of NPCC immaturity via T3 release.
Strong Eastern Pacific El Niño events largely determine the spread in ENSO Amplitude and Asymmetry in Climate Models
Authors: Bayr Tobias , Lübbecke Joke F. , Vialard Jérôme , Latif Mojib
Published: September 2023

The El Niño/Southern Oscillation (ENSO) exhibits a considerable asymmetry in Sea Surface Temperature anomalies (SSTa), as El Niño events tend to be stronger and centered further east than La Niña events. Here we show by observations and climate models that the southward shift of the Intertropical Convergence Zone (ITCZ) onto the equator during strong Eastern Pacific (EP) El Niño events is essential for ENSO asymmetry, as this shifts the wind-SST feedback eastward by up to 15° compared to Central Pacific (CP) El Niño events, explaining the more eastern center in SSTa. Further, the thermocline feedback is enhanced in the east and in turn the Bjerknes feedback, which explains the large amplitude of the EP El Niño events. These results are confirmed by the CESM1 Large Ensemble. Further, it shows a statistically significant and strong (r ~ 0.8) link between internally generated differences in the fraction of strong EP events in 50-years long realizations and their overall ENSO amplitude and asymmetry. Finally, we analyze ENSO amplitude and asymmetry in preindustrial control simulations of 32 models participating in the Coupled Model Intercomparison Project phase 6 (CMIP6). The spread in ENSO amplitude across the model ensemble can be largely explained by the fraction of the strong EP El Niños. Half of the models strongly underestimate the ENSO asymmetry, which can be partly explained by the equatorial Pacific cold SST bias that hampers the southward migration of the ITCZ onto the equator during El Niño events, leading to fewer strong EP El Niño events.
Application of in-situ process monitoring to optimize laser parameters during laser powder bed fusion printing of Ti-6Al-4V parts with overhang structures
Authors: Power John James , Humphreys Owen , Hartnett Mark , Egan Darragh , Dowling Denis P.
Published: September 2023

Geometrically complex features, such as overhang structures, can be a challenge in laser powder bed fusion (L-PBF), as they can be associated with print defects, such as porosity. In this study, Ti-6Al-4V alloy overhang structures were fabricated using an L-PBF system. Differences were observed in the microstructure in the region around the overhang structures, compared with that observed for the bulk alloy. These included larger grain sizes and a less homogenous microstructure in the print layers closest to overhang structures. It is hypothesized that these microstructural changes are associated with the excess heat generated in the overhang region due to the decreased thermal conductivity of the powder immediately below the print layers, compared with solid alloy. Also observed was an increased level of porosity (up to 0.08%) in the overhang print alloy, compared with the corresponding <0.02% in the alloy bulk. During printing, in-situ process monitoring of the meltpool emissions was obtained in the near-infrared range and correlated with the properties of the printed parts.
This in-process data assisted in selecting optimal laser processing conditions in the first fifteen layers above the overhang to prevent meltpool overheating. By systematically controlling the laser energy during the printing of L-PBF overhang structures, the level of porosity was reduced to match that of the bulk alloy. There was also an associated reduction in the roughness of the overhang itself, with its Ra decreasing from 62.4 ± 7.3 µm to 7.5 ± 1.9 µm.