bluuebunny/crossref_metadata_2025 huggingface dataset:
Differential Strategies of Two Arbuscular Mycorrhizal Fungi Varieties in the Protection of Lycium ruthenicum under Saline–Alkaline Stress
Authors: Zheng Xu , Li Ao , Nie Ruining , Wu Chengxu , Ji Xinying , Tang Jiali , Zhang Junpei
Published: August 2024

To delve into the growth and physiological adaptations exhibited by the economically vital black wolfberry (Lycium ruthenicum) upon inoculation with arbuscular mycorrhizal fungi (AMF) under varying levels of saline–alkaline stress A series of pot experiments were conducted in a gradient saline–alkaline environment (0, 200, 400 mM NaCl: NaHCO3 = 1:1). One-year-old cuttings of black wolfberry, inoculated with two AMF species—Funneliformis mosseae (Fm) and Rhizophagus intraradices (Ri)—served as the experimental material, enabling a comprehensive analysis of seedling biomass, chlorophyll content, antioxidant enzyme activities, and other crucial physiological parameters. This study demonstrated that both Fm and Ri could form a symbiotic relationship with the root of Lycium ruthenicum. Notably, Fm inoculation significantly bolstered the growth of the underground parts, while exhibiting a remarkable capacity to scavenge reactive oxygen species (ROS), thereby effectively mitigating membrane oxidative damage induced by stress. Additionally, Fm promoted the accumulation of abscisic acid (ABA) in both leaves and roots, facilitating the exclusion of excess sodium ions from cells. Ri Inoculation primarily contributed to an enhancement in the chlorophyll b (Chlb) content, vital for sustaining photosynthesis processes. Furthermore, Ri’s ability to enhance phosphorus (P) absorption under stressful conditions ensured a steady influx of essential nutrients. These findings point to different strategies employed for Fm and Ri inoculation. To holistically assess the saline–alkaline tolerance of each treatment group, a membership function analysis was employed, ultimately ranking the salt tolerance as Fm > Ri > non-mycorrhizal (NM) control. This finding holds paramount importance for the screening of highly resilient Lycium ruthenicum strains and offers invaluable theoretical underpinnings and technical guidance for the remediation of saline–alkaline soils, fostering sustainable agricultural practices in challenging environments.
Aeolian Environment Regionalization in Xinjiang and Suggestions for Sand Prevention in Typical Areas
Authors: Zhou Jie , Ren Hongjing , Han Beibei , Zhao Yazhou , Wang Haifeng
Published: August 2024

The Xinjiang region is prone to frequent and complex wind and sand disasters, which present a significant challenge to the sustainable development of local areas. This research uses multi-source data to analyze the spatial distribution of the aeolian environment in Xinjiang, establishes a four-level zoning scheme, and proposes recommendations for ecological management and engineering and control. Results indicate that (1) Xinjiang’s aeolian environment and its types exhibit spatial heterogeneity. The aeolian environment types display a high concentration in the eastern region and a low concentration in the western region. Furthermore, the aeolian environment types are concentrated in the basin region. Moreover, the aeolian environment types exhibit a meridional distribution pattern. (2) A four-level zoning system for aeolian environments in Xinjiang was developed, comprising two first-level zones, seven s-level subzones, 22 third-level wind zones, and 31 fourth-level subdivisions. (3) A structural model for a highway sand control system is proposed for aeolian environment types of subdivisions, including fixing-based, combined blocking and fixing, wind-blocking and sand-transferring, and combined blocking and fixing–transferring. The aeolian environment regionalization program proposed in this study can be a scientific reference for relevant departments in formulating and implementing sand prevention and control planning.
Efficient Regeneration of Graphite from Spent Lithium-Ion Batteries through Combination of Thermal and Wet Metallurgical Approaches
Authors: Yu Riquan , Zhou Changyou , Zhou Xiangyang , Yang Juan , Tang Jingjing , Zhang Yaguang
Published: August 2024

With the large-scale application of lithium-ion batteries (LIBs) in various fields, spent LIBs are considered one of the most important secondary resources. Few studies have focused on recycling anode materials despite their high value. Herein, a new efficient recycling and regeneration method of spent anode materials through the combination of thermal and wet metallurgical approaches and restored graphite performance is presented. Using this method, the lithium recycling ratio from spent anode materials reaches 87%, with no metal impurities detected in the leaching solution. The initial Coulombic efficiency of the recycled graphite (RG) materials is 90.5%, with a reversible capacity of 350.2 mAh/g. Moreover, RG shows better rate performance than commercial graphite. The proposed method is simple and efficient and does not involve toxic substances. Thus, it has high economic value and application potential in graphite recycling from spent LIBs.
Effects of Carbon Nanotubes on Mechanical Strength, Damage Process, and Microstructure of Lithium Tailing Backfilling
Authors: Hu Shufen , Guan Huadong , Wu Cai , Lu Yani , Zhu Daopei
Published: August 2024

In this study, common multiwalled and carboxylated carbon nanotubes (CNTs) were added to the cemented lithium tailings backfill (CLTB). The effects of CNTs on the mechanical properties, hydration products, damage process, and microstructure of CLTB specimens were studied by uniaxial compression (UCS), infrared spectroscopy (FT-IR), and scanning electron microscopy (SEM). The experimental results show that the addition of CNTs effectively increased the compressive strength compared with the blank control group. When the concentration was 0.05–0.20%, the compressive strength was proportional to the content, the optimal addition amount was 0.2%, and the enhancement effect was 75% and 95.31%, respectively. The FT-IR results indicate that the addition of CNTs increased the total amount of the hydration product but did not affect its type. The hydration of the three-dimensional reciprocal penetration network formed by moderate amounts of CNTs has a positive effect on the mechanical strength of CLTB specimens.
Experimental Study on the Effects of Tapioca Starch on Cement Mortar Quality Improvement
Authors: Jang Chang-Hwan , Kim Yong-Jic , Oh Sung-Rok
Published: August 2024

In this study, the effect of tapioca starch (TP) on mortar was evaluated by incorporating TP into the mortar mixture. The evaluation involved analyzing the mortar’s quality characteristics, performance, and fundamental quality improvements. The addition of TP resulted in a decrease in flow, which was attributed to increased viscosity. Specifically, a 10% reduction in flow was observed with a 0.025% increase in TP content. After 28 days, the impact of TP on the compressive strength of the mortar remained consistent, regardless of the TP amount. However, within the first 3 days, higher TP content accelerated strength development, with early compressive strength increasing by up to 20% at a 0.050% TP level. Additionally, bond strength improved by approximately 60% at a 0.050% TP concentration, and final shrinkage was reduced by 5%.
Toward Metallized Pellets for Steelmaking by Hydrogen Cooling Reduction: Effect of Gas Flow Rate
Authors: Fan Wanlong , Peng Zhiwei , Tian Ran , Luo Guanwen , Yi Lingyun , Rao Mingjun
Published: August 2024

This study proposed a strategy to prepare metalized pellets for direct steelmaking by hydrogen cooling reduction (HCR) of iron ore pellets with a focus on the effect of H2 flow rate on the process. It was demonstrated that increasing H2 flow rate could effectively enhance the reduction performance of iron ore pellets. However, due to the influence of the countercurrent diffusion resistance of gas molecules, too high H2 flow rate no longer promoted the reduction of the pellets when the maximum reduction rate was reached. The reduction swelling index (RSI) of the pellets initially increased and then decreased with increasing H2 flow rate. This change was associated with the decreased content of Fe2SiO4 in the metalized pellets and the changes in porosity and iron particle size. The compressive strength (CS) decreased continuously, showing a sharp decline when the H2 flow rate reached 0.6 L/min. It was attributed to the significant increases in porosity and average pore size of the metalized pellets, with the presence of surface cracks. When the H2 flow rate was 0.8 L/min, the metalized pellets had the optimal performance, namely, reduction degree of 91.45%, metallization degree of 84.07%, total iron content of 80.67 wt%, RSI of 4.66%, and CS of 1265 N/p. The findings demonstrated the importance of controlling the H2 flow rate in the preparation of metallized pellets by HCR.
Modelling and Design Optimization of a Novel Compliant XY Positioner for Vibration-Assisted CNC Milling
Authors: Dang Minh Phung , Tran Chi Thien , Le Hieu Giang , Tran Vo Quoc Anh , Tran Hong Van
Published: August 2024

Vibration-assisted machining, known as hybrid processing technology, offers several benefits over conventional machining methods. However, developing mechanical structure designs to generate a non-resonant frequency source remains challenging. The objective of this study is to propose a novel design for an XY flexure positioner by combining the pseudo-rigid-body model with the Lagrange technique, finite element analysis and Crayfish optimization algorithm. Firstly, the mechanism was designed by combining a hybrid amplifier and parallel driving mechanism integrated with right circular hinges to increase the natural frequency and precision for potential application to VAM CNC milling. Then, the analytical model was established by the pseudo-rigid-body and Lagrange method. Next, the theoretical result was verified by finite element analysis. The first natural frequency results of theory and FEM methods were found at 990.74 Hz and 1058.5 Hz, respectively. The error between the two methods was 6.4%, demonstrating a reliable modeling approach. Based on the analytical equations, the Crayfish optimization algorithm was utilized for optimizing the main design variables of the mechanism. Next, the prototype was fabricated. The results showed that the experimental and simulated frequencies were 1127.62 Hz and 1216.6 Hz, with an error between the two methods of 7.31%. Finally, the workpiece was installed on the prototype and a real vibration-assisted CNC milling process was carried out in the frequency range [700 Hz, 1000 Hz]. The best surface roughness of the specimen was achieved at a frequency of 900 Hz with a Ra of 0.287 µm. This demonstrates that the proposed XY mechanism is an effective structure for generating a non-resonant frequency source for vibration-assisted machining.
Cutting-Edge Microwave Sensors for Vital Signs Detection and Precise Human Lung Water Level Measurement
Authors: Abd El-Hameed Anwer S. , Elsheakh Dalia M. , Elashry Gomaa M. , Abdallah Esmat A.
Published: August 2024

In this article, a comprehensive review is presented of recent technological advancements utilizing electromagnetic sensors in the microwave range for detecting human vital signs and lung water levels. With the main objective of improving detection accuracy and system robustness, numerous advancements in front-end architecture, detection techniques, and system-level integration have been reported. The benefits of non-contact vital sign detection have garnered significant interest across a range of applications, including healthcare monitoring and search and rescue operations. Moreover, some integrated circuits and portable systems have lately been shown off. A comparative examination of various system architectures, baseband signal processing methods, system-level integration strategies, and possible applications are included in this article. Going forward, researchers will continue to focus on integrating radar chips to achieve compact form factors and employ advanced signal processing methods to further enhance detection accuracy.
Adverse Events in Osimertinib Treatment for EGFR-Mutated Non-Small-Cell Lung Cancer: Unveiling Rare Life-Threatening Myelosuppression
Authors: Shalata Walid , Abu Jama Ashraf , Dudnik Yulia , Abu Saleh Omar , Shalata Sondos , Tourkey Lena , Sheva Kim , Meirovitz Amichay , Yakobson Alexander
Published: August 2024

Recent advancements in targeted therapies for non-small-cell lung cancer (NSCLC), specifically focusing on epidermal growth factor receptor (EGFR) mutations, have revolutionized treatment strategies. Osimertinib, an approved therapy for metastatic NSCLC with EGFR mutations, highlights remarkable efficacy but also harbors the potential for severe adverse events, whose rarity or lack of precedence may mask their criticality. This article delves into the exploration of adverse events linked to osimertinib, shedding light on a rare yet life-threatening occurrence: severe myelosuppression. A case study detailing a patient with EGFR-mutated NSCLC exhibiting a robust treatment response but experiencing severe myelosuppression following osimertinib initiation is presented. Immediate discontinuation of osimertinib alongside concurrent blood transfusions facilitated toxicity recovery, prompting a successful reduction in myelosuppression severity upon re-administration at a lowered dosage.
Mixed-Matrix Organo-Silica–Hydrotalcite Membrane for CO2 Separation Part 1: Synthesis and Analytical Description
Authors: Bünger Lucas , Garbev Krassimir , Ullrich Angela , Stemmermann Peter , Stapf Dieter
Published: August 2024

Hydrotalcite exhibits the capability to adsorb CO2 at elevated temperatures. High surface area and favorable coating properties are essential to harness its potential for practical applications. Stable alcohol-based dispersions are needed for thin film applications of mixed membranes containing hydrotalcite. Currently, producing such dispersions without the need for delamination and dispersing agents is a challenging task. This work introduces, for the first time, a manufacturing approach to overcoming the drawbacks mentioned above. It includes a synthesis of hydrotalcite nanoparticles, followed by agent-free delamination of their layers and final dispersion into alcohol without dispersing agents. Further, the hydrotalcite-derived sorption agent is dispersed in a matrix based on organo-silica gels derived from 1,2-bis(triethoxysilyl)ethane (BTESE). The analytical results indicate that the interconnection between hydrotalcite and BTESE-derived gel occurs via forming a strong hydrogen bonding system between the interlayer species (OH groups, CO32−) of hydrotalcite and oxygen and silanol active gel centers. These findings lay the foundation for applications involving incorporating hydrotalcite-like compounds into silica matrices, ultimately enabling the development of materials with exceptional mass transfer properties. In part 2 of this study, the gas separation performance of the organo-silica and the hydrotalcite-like materials and their combined form will be investigated.
Investigating the Link between Intermediate Metabolism, Sexual Dimorphism, and Cardiac Autonomic Dysfunction in Patients with Type 1 Diabetes
Authors: Insenser María Rosa , Nattero-Chávez Lía , Luque-Ramírez Manuel , Quiñones Sara de Lope , Quintero-Tobar Alejandra , Samino Sara , Amigó Núria , Dorado Avendaño Beatriz , Fiers Tom , Escobar-Morreale Héctor F.
Published: August 2024

Sexual dimorphism influences cardiovascular outcomes in type 1 diabetes (T1D), with women facing a higher relative risk of macrovascular events compared to men, especially after menopause. This study hypothesizes that abnormalities in intermediate metabolism may be associated with cardiac autonomic neuropathy (CAN) in T1D. We aim to assess low molecular weight metabolites (LMWM) as markers of CAN in T1D, considering the effects of sexual dimorphism and age. In this cross-sectional study, we included 323 subjects with T1D (147 women and 176 men), with a mean age of 41 ± 13 years. A total of 44 women and 41 men were over 50 years old. CAN was assessed using Ewing’s tests, and serum metabolites were analyzed by proton nuclear magnetic resonance spectroscopy (1H-NMR). Patients with CAN had lower levels of valine, isoleucine, and threonine, and higher levels of lactate, compared to those without CAN. These differences persisted after adjusting for BMI and estimated glucose disposal rate (eGDR). In a logistic regression model (R² = 0.178, p < 0.001), the main determinants of CAN included isoleucine [Exp(β) = 0.972 (95% CI 0.952; 0.003)], age [Exp(β) = 1.031 (95% CI 1.010; 1.053)], A1c [Exp(β) = 1.361 (95% CI 1.058; 1.752)], and microangiopathy [Exp(β) = 2.560 (95% CI 1.372; 4.778)]. Sex influenced LMWM profiles, with over half of the metabolites differing between men and women. However, no interactions were found between CAN and sex, or between sex, age, and CAN, on metabolomics profiles. Our findings suggest an association between CAN and LMWM levels in T1D. The sexual dimorphism observed in amino acid metabolites was unaffected by the presence of CAN.
Branched-Chain Amino Acids, Alanine, and Thyroid Function: A Cross-Sectional, Nuclear Magnetic Resonance (NMR)-Based Approach from ELSA-Brasil
Authors: Janovsky Carolina Castro Porto Silva , Meneghini Vandrize , Tebar William , Martins Joao Roberto Maciel , Sgarbi José Augusto , Teixeira Patrícia de Fatima dos Santos , Jones Steven R. , Blaha Michael J. , Toth Peter P. , Lotufo Paulo A. , Bittencourt Marcio S. , Santos Raul D. , Santos Itamar S. , Chaker Layal , Bensenor Isabela M.
Published: August 2024

The association of thyroid function with essential and non-essential amino acids is understudied, despite their common metabolic roles. Thus, our aim was to evaluate the association of thyroid function with the levels of branched-chain amino acids (BCAAs—leucine, isoleucine, and valine) and of alanine in the general population. We utilized data from the São Paulo research center of ELSA-Brasil, a longitudinal population-based cohort study. Thyroid parameters included thyroid stimulating hormone (TSH), free T4 and free T3 levels, and the FT4:FT3 ratio. BCAAs and alanine were analyzed on a fully automated NMR platform. The current analysis included euthyroid participants and participants with subclinical hyperthyroidism and hypothyroidism. We used Pearson’s coefficient to quantify the correlation between thyroid-related parameters and amino acids. Linear regression models were performed to analyze whether thyroid parameters were associated with BCAAs and alanine levels. We included 4098 participants (51.3 ± 9.0 years old, 51.5% women) in this study. In the most adjusted model, higher levels of TSH were associated with higher levels of alanine, FT4 levels were inversely associated with isoleucine levels, FT3 levels were statistically significant and positively associated with valine and leucine, and the T3:T4 ratio was positively associated with all amino acids. We observed that subclinical hypothyroidism was positively associated with isoleucine and alanine levels in all models, even after full adjustment. Our findings highlight the association of subclinical hypothyroidism and thyroid-related parameters (including TSH, free T4, free T3, and FT4:FT3 ratio) with BCAAs and alanine. Further studies are needed to explore the mechanisms underlying this association. These insights contribute to our understanding of the influence of thyroid-related parameters on BCAA and alanine metabolism.
Micro-Opto-Electro-Mechanical Systems for High-Precision Displacement Sensing: A Review
Authors: Xin Chenguang , Xu Yingkun , Zhang Zhongyao , Li Mengwei
Published: August 2024

High-precision displacement sensing has been widely used across both scientific research and industrial applications. The recent interests in developing micro-opto-electro-mechanical systems (MOEMS) have given rise to an excellent platform for miniaturized displacement sensors. Advancement in this field during past years is now yielding integrated high-precision sensors which show great potential in applications ranging from photoacoustic spectroscopy to high-precision positioning and automation. In this review, we briefly summarize different techniques for high-precision displacement sensing based on MOEMS and discuss the challenges for future improvement.
A Holistic Approach to Producing Anti-Vibrio Metabolites by an Endosymbiotic Dinoflagellate Using Wastewater from Shrimp Rearing
Authors: Oliveira Carlos Yure B. , Abreu Jéssika L. , Brandão Barbara C. , Oliveira Deyvid Willame S. , de Sena Pedro Rodrigues , da Silva Weverson Ailton , Araújo Evando S. , Rörig Leonardo R. , de Almeida Costa Gisely Karla , Silva Suzianny Maria B. C. , Müller Marius N. , Tribuzi Giustino , Gálvez Alfredo O.
Published: August 2024

The aquaculture industry requires green solutions to solve several environmental challenges, including adequate wastewater remediation and natural drug applications to treat bacteria- and virus-related diseases. This study investigated the feasibility of cultivating the dinoflagellate Durusdinium glynnii in aquaculture wastewater from shrimp rearing in a synbiotic system (AWW-SS), with different dilutions of f/2 medium (FM). Interestingly, D. glynnii demonstrated enhanced growth in all AWW–SS treatments compared to the control (FM). The highest growth rates were achieved at AWW-SS:FM dilutions of 75:25 and 50:50. The removal of total nitrogen and total phosphorus reached 50.1 and 71.7%, respectively, of the crude AWW–SS. Biomass extracts of D. glynnii grown with AWW–SS were able to inhibit the growth of the bacteria Vibrio parahaemolyticus (inhibition zone of 10.0 ± 1.7 mm) and V. vulnificus (inhibition zone of 11.7 ± 1.5 mm). The presented results demonstrate that the dinoflagellate D. glynnii is a potential candidate for the development of circularity for sustainable aquaculture production, particularly by producing anti–Vibrio compounds at a near-zero cost.
Antimicrobial Resistance and Genomic Characterization of Campylobacter jejuni and Campylobacter coli Isolated from Retail Chickens in Beijing, China
Authors: Bai Yao , Ma Jiaqi , Li Fengqin , Yang Baowei , Ren Xiu , Wang Yeru , Hu Yujie , Dong Yinping , Wang Wei , Zhang Jing , Yan Shaofei , Cui Shenghui
Published: August 2024

Objective Campylobacter species are the main causes of foodborne illness worldwide, posing significant threats to public health. This study aimed to investigate the antibiotic resistance and genomic characterization of C. jejuni/C.coli from retail chickens in Beijing. Methods Antimicrobial susceptibility testing was conducted on 126 C. jejuni/C. coli isolated from retail chickens in Beijing, following CLSI protocols. Whole genomes of all isolates were sequenced using the Illumina platform. Results More C. coli (83.82%) showed multi-drug resistance than C. jejuni (8.62%). Genomic analysis demonstrated 42 sequence types (STs) and 12 clonal complexes (CCs), from which CC828 and CC52 were dominant. cdtA, cdtB and cdtC encoding cytotoxic protein were present spontaneously in most C. jejuni but not found in any C. coli isolates. The abundances of antibiotic resistance genes (ARGs) and virulence genes (VGs) in C. jejuni and C. coli were significantly different, with ARGs numbered in C. coli and VGs in C. jejuni. Conclusions High prevalence of multi-drug resistance C. coli and C. jejuni isolated from Beijing chickens were challenging clinical antibiotic usages in the treatment of Campylobacter infection. The surveillance of particular C. jejuni and C. coli STs correlated with higher resistance and virulence needs to be strengthened in the future.
Editorial for the Special Issue “Formation and Evolution of the Continental Crust in North China Craton during Precambrian”
Authors: Liu Jin , Qian Jiahui , Liu Xiaoguang
Published: August 2024

The North China Craton (NCC) preserves ancient rocks dating back to ca [...]
Micro-Structure Engineering in Pd-InOx Catalysts and Mechanism Studies for CO2 Hydrogenation to Methanol
Authors: Zhao Fengwang , Liang Gemeng , Yang Xiaoli , Lei Yang , Jin Fayi , Xu Leilei , Zhang Chuanhui , Jiang Wei , Ben Haoxi , Li Xingyun
Published: August 2024

Significant interest has emerged for the application of Pd-In2O3 catalysts as high-performance catalysts for CO2 hydrogenation to CH3OH. However, precise active site control in these catalysts and understanding their reaction mechanisms remain major challenges. In this investigation, a series of Pd-InOx catalysts were synthesized, revealing three distinct types of active sites: In-O, Pd-O(H)-In, and Pd2In3. Lower Pd loadings exhibited Pd-O(H)-In sites, while higher loadings resulted in Pd2In3 intermetallic compounds. These variations impacted catalytic performance, with Pd-O(H)-In catalysts showing heightened activity at lower temperatures due to the enhanced CO2 adsorption and H2 activation, and Pd2In3 catalysts performing better at elevated temperatures due to the further enhanced H2 activation. In situ DRIFTS studies revealed an alteration in key intermediates from *HCOO over In-O bonds to *COOH over Pd-O(H)-In and Pd2In3 sites, leading to a shift in the main reaction pathway transition and product distribution. Our findings underscore the importance of active site engineering for optimizing catalytic performance and offer valuable insights for the rational design of efficient CO2 conversion catalysts.
Metabolite Variations during the First Weeks of Growth of Immature Citrus sinensis and Citrus reticulata by Untargeted Liquid Chromatography–Mass Spectrometry/Mass Spectrometry Metabolomics
Authors: Deschamps Estelle , Durand-Hulak Marie , Castagnos Denis , Hubert-Roux Marie , Schmitz Isabelle , Froelicher Yann , Afonso Carlos
Published: August 2024

Immature citruses are an important resource for the pharmaceutical industry due to their high levels of metabolites with health benefits. In this study, we used untargeted liquid chromatography–mass spectrometry (LC-MS) metabolomics to investigate the changes associated with fruit size in immature citrus fruits in the first weeks of growth. Three orange cultivars (Citrus sinensis ‘Navel’, Citrus sinensis ‘Valencia’, and Citrus sinensis ‘Valencia Late’) and a mandarin (Citrus reticulata Blanco ‘Fremont’) were separated into eight fruit sizes, extracted, and analyzed. Statistical analyses revealed a distinct separation between the mandarin and the oranges based on 56 metabolites, with an additional separation between the ‘Navel’ orange and the ‘Valencia’ and ‘Valencia Late’ oranges based on 21 metabolites. Then, metabolites that evolved significantly with fruit size growth were identified, including 40 up-regulated and 31 down-regulated metabolites. This study provides new insights into the metabolite modifications of immature Citrus sinensis and Citrus reticulata in the first weeks of growth and emphasizes the significance of including early sampled fruits in citrus maturation studies.
Synthesis of Benzofuran Derivatives via a DMAP-Mediated Tandem Cyclization Reaction Involving ortho-Hydroxy α-Aminosulfones
Authors: Zhu Rong-Rong , Hou Xi-Qiang , Du Da-Ming
Published: August 2024

An efficient cascade cyclization strategy was developed to synthesize aminobenzofuran spiroindanone and spirobarbituric acid derivatives utilizing 2-bromo-1,3-indandione, 5-bromo-1,3-dimethylbarbituric acid, and ortho-hydroxy α-aminosulfones as substrates. Under the optimized reaction conditions, the corresponding products were obtained with high efficiency, exceeding 95% and 85% yields for the respective derivatives. This protocol demonstrates exceptional substrate versatility and robust scalability up to the Gram scale, establishing a stable platform for the synthesis of 3-aminobenzofuran derivative. The successful synthesis paves the way for further biological evaluations with potential implications in scientific research.
Enhancing Reflective and Conversational User Engagement in Argumentative Dialogues with Virtual Agents
Authors: Aicher Annalena , Matsuda Yuki , Yasumoto Keichii , Minker Wolfgang , André Elisabeth , Ultes Stefan
Published: August 2024

In their process of information seeking, human users tend to selectively ignore information that contradicts their pre-existing beliefs or opinions. These so-called “self-imposed filter bubbles” (SFBs) pose a significant challenge for argumentative conversational agents aiming to facilitate critical, unbiased opinion formation on controversial topics. With the ultimate goal of developing a system that helps users break their self-imposed filter bubbles (SFBs), this paper aims to investigate the role of co-speech gestures, specifically examining how these gestures significantly contribute to achieving this objective. This paper extends current research by examining methods to engage users in cooperative discussions with a virtual human-like agent, encouraging a deep reflection on arguments to disrupt SFBs. Specifically, we investigate the agent’s non-verbal behavior in the form of co-speech gestures. We analyze whether co-speech gestures, depending on the conveyed information, enhance motivation, and thus conversational user engagement, thereby encouraging users to consider information that could potentially disrupt their SFBs. The findings of a laboratory study with 56 participants highlight the importance of non-verbal agent behaviors, such as co-speech gestures, in improving users’ perceptions of the interaction and the conveyed content. This effect is particularly notable when the content aims to challenge the user’s SFB. Therefore, this research offers valuable insights into enhancing user engagement in the design of multimodal interactions with future cooperative argumentative virtual agents.
Design of All-Optical D Flip Flop Memory Unit Based on Photonic Crystal
Authors: Pugachov Yonatan , Gulitski Moria , Malka Dror
Published: August 2024

This paper proposes a unique configuration for an all-optical D Flip Flop (D-FF) utilizing a quasi-square ring resonator (RR) and T-Splitter, as well as NOT and OR logic gates within a 2-dimensional square lattice photonic crystal (PC) structure. The components realizing the all-optical D-FF comprise of optical waveguides in a 2D square lattice PC of 45 × 23 silicon (Si) rods in a silica (SiO2) substrate. The utilization of these specific materials has facilitated the fabrication process of the design, diverging from alternative approaches that employ an air substrate, a method inherently unattainable in fabrication. The configuration underwent examination and simulation utilizing both plane-wave expansion (PWE) and finite-difference time-domain (FDTD) methodologies. The simulation outcomes demonstrate that the designed waveguides and RR effectively execute the operational principles of the D-FF by guiding light as intended. The suggested configuration holds promise as a logic block within all-optical arithmetic logic units (ALUs) designed for digital computing optical circuits. The design underwent optimization for operation within the C-band spectrum, particularly at 1550 nm. The outcomes reveal a distinct differentiation between logic states ‘1’ and ‘0’, enhancing robust decision-making on the receiver side and minimizing logic errors in the photonic decision circuit. The D-FF displays a contrast ratio (CR) of 4.77 dB, a stabilization time of 0.66 psec, and a footprint of 21 μm × 12 μm.
Hierarchical WS2-WO3 Nanohybrids with Flower-like p-n Heterostructures for Trimethylamine Detection
Authors: Meng Dan , Ran Shunjiang , Zhang Lei , San Xiaoguang , Zhang Yue , Zheng Yu , Qi Jian
Published: August 2024

The detection of trimethylamine (TMA) is critically important due to its toxic and flammable nature, which poses significant risks to human health and the environment. However, achieving high response, rapid kinetics, selectivity, and low operating temperatures in TMA sensing remains challenging. In this study, WS2/WO3 nanohybrids with flower-like hierarchical structures were synthesized via an in situ sulfurization process, utilizing varying amounts of thioacetamide to control the sulfurization state of WO3. These novel hierarchical WS2/WO3 nanohybrids exhibit remarkable selectivity towards TMA, as well as rapid response and recovery characteristics. Specially, the optimal WS2/WO3 sensor, composed of 5% WS2/WO3 nanohybrids, demonstrates exceptional TMA sensing performance, including a high response (19.45 at 10 ppm), good repeatability, reliable long-term stability, and a low theoretical detection limit (15.96 ppb). The superior sensing capabilities of the WS2/WO3 nanohybrids are attributed to the formation of p-n heterojunctions at the interface, the unique hierarchical structures, and the catalytic activity of WS2. Overall, this work provides a straightforward and versatile approach for synthesizing multifunctional nanomaterials by combining metal oxide micro-flowers with transition metal dichalcogenide nanoflakes for applications in monitoring TMA in complex environments.
The Influence of PEG 4000 on the Physical and Microstructural Properties of 58S Bioactive Glasses
Authors: Lixandru Matei Ioana Lavinia , Sava Bogdan Alexandru , Sarosi Codruta , Dușescu-Vasile Cristina , Popovici Daniela Roxana , Ionescu Andreea Iuliana , Bomboș Dorin , Băjan Marian , Doukeh Rami
Published: August 2024

Bioactive glass is currently considered a material with a high biocompatibility and has been used both in the field of bone regeneration and in the preparation of cosmetic products with the controlled release of active compounds. The present work involved a study on the synthesis of bioglass using the sol–gel process. The study aims to evaluate the influence of the treatment of bioglass with Polyethylene glycol 4000 (PEG 4000) on its main characteristics. The surface characteristics of this material were obtained by nitrogen adsorption/desorption analysis, using the standard BET (Brunauer–Emmett–Teller) equation, the crystallinity by XRD (X-ray diffraction) analysis, the surface structure by SEM (Scanning Electron Microscope), thermal stability by TGA (ThermoGravimetric Analyses), and chemical bonds changes by FTIR (Fourier transform infrared) spectroscopy. After treatment with PEG 4000, the average diameter of the pores increased insignificantly, the crystallinity peak disappeared, and the SEM analysis highlighted several clusters of very small sizes.
Reply to Child, R. Comment on “Cesak et al. Carnosine and Beta-Alanine Supplementation in Human Medicine: Narrative Review and Critical Assessment. Nutrients 2023, 15, 1770”
Authors: Cesak Ondrej , Vostalova Jitka , Vidlar Ales , Bastlova Petra , Student Vladimir
Published: August 2024

Thank you very much for your comment [...]
Puerarin Alleviates Blood Pressure via Inhibition of ROS/TLR4/NLRP3 Inflammasome Signaling Pathway in the Hypothalamic Paraventricular Nucleus of Salt-Induced Prehypertensive Rats
Authors: Gao Hong-Li , Yang Yu , Tian Hua , Xu Shen-Liang , Li Bo-Wen , Fu Li-Yan , Liu Kai-Li , Shi Xiao-Lian , Kang Yu-Ming , Yu Xiao-Jing
Published: August 2024

Background: Puerarin is an isoflavone compound isolated from the roots of a leguminous plant, the wild kudzu. Various functional activities of this compound in multiple diseases have been reported. However, the effect and mechanism of puerarin in improving blood pressure remain non-elucidated. Purpose: The current study was designed to assess the preventive effects of puerarin on the onset and progression of hypertension and to verify the hypothesis that puerarin alleviates blood pressure by inhibiting the ROS/TLR4/NLRP3 inflammasome signaling pathway in the hypothalamic paraventricular nucleus (PVN) of salt-induced prehypertensive rats. Methods: Male Dahl salt-sensitive rats were fed low NaCl salt (3% in drinking water) for the control (NS) group or 8% (HS) to induce prehypertension. Each batch was divided into two group and treated by bilateral PVN microinjection with either artificial cerebrospinal fluid or puerarin through a micro-osmotic pump for 6 weeks. The mean arterial pressure (MAP) was recorded, and samples were collected and analyzed. Results: We concluded that puerarin significantly prevented the elevation of blood pressure and effectively alleviated the increase in heart rate caused by high salt. Norepinephrine (NE) in the plasma of salt-induced prehypertensive rats also decreased upon puerarin chronic infusion. Additionally, analysis of the PVN sample revealed that puerarin pretreatment decreased the positive cells and gene level of TLR4 (Toll-like receptor 4), NLRP3, Caspase-1 p10, NOX2, MyD88, NOX4, and proinflammatory cytokines in the PVN. Puerarin pretreatment also decreased NF-κBp65 activity, inhibited oxidative stress, and alleviated inflammatory responses in the PVN. Conclusion: We conclude that puerarin alleviated blood pressure via inhibition of the ROS/TLR4/NLRP3 inflammasome signaling pathway in the PVN, suggesting the therapeutic potential of puerarin in the prevention of hypertension.
Gender Difference in the Impact of Total Energy Intake on the Association between Low Fiber Intake and Mental Health in Middle-Aged and Older Adults
Authors: Cho Sinyoung , Park Minseon
Published: August 2024

The effect of dietary fiber intake on mental health is controversial. This study aimed to examine the association of fiber intake with mental health in Korean adults. This cross-sectional study included 11,288 participants aged ≥40 years who participated in the Korean Genome and Epidemiology Study (2004–2013). Fiber intake was assessed using a food frequency questionnaire and categorized into sex-specific quintiles. Multiple logistic regression models were used to investigate the association between the lowest quintile of fiber intake and poor mental health. Mental health was assessed using acute stress perception, the Psychosocial Well-Being Index-Short Form, self-rated health, and the Center for Epidemiological Studies–Depression Scale in Korea. Compared to those with higher fiber intake, having the lowest quintile of fiber intake was associated with higher odds of poor mental health risk, a higher risk of high-stress perception, poor psychosocial distress in males, poor psychosocial distress, and depression in females. Low fiber intake had profound negative mental health effects on males with high total energy intake and females with low total energy intake. In conclusion, there is a gender difference in the impact of total energy intake on the deleterious effect of low fiber intake on mental health.
The Effects of Caloric Restriction and Clinical Psychological Intervention on the Interplay of Gut Microbial Composition and Stress in Women
Authors: Bellach Luise , Kautzky-Willer Alexandra , Heneis Kathrin , Leutner Michael , Kautzky Alexander
Published: August 2024

Both mental and metabolic disorders are steadily becoming more prevalent, increasing interest in non-pharmacological lifestyle interventions targeting both types of disorders. However, the combined effect of diet and psychological interventions on the gut microbiome and mental health outcomes remains underexplored. Thus, in this study, we randomized 41 women into two caloric restriction (CR) dietary groups, namely very-low-calorie diet (VLCD) and F.X. Mayr diet (FXM). The patients were then further randomized to either receive clinical psychological intervention (CPI) or no CPI. Blood and fecal samples were collected before and after two weeks of CR. Psychometric outcomes were assessed using the Perceived Stress Scale (PSS), Brief Symptom Index (BSI), and Burnout Dimension Inventory (BODI). Stool samples underwent 16S-rRNA sequencing. Upon two weeks of CR, α-diversity decreased overall and longitudinal PERMANOVA models revealed significant shifts in β-diversity according to diet, CPI, age, and body-mass-index. Furthermore, Agathobacter, Fusicatenibacter, and Subdoligranulum decreased in abundance. However, the Oscillibacter genus was enriched solely in FXM. CPI had a negligible effect on the microbiome. Dimension reduction models revealed clusters of taxa which distinctly associated with psychometric outcomes. Members of the Oscillospiraceae family were linked to favorable psychometric outcomes after two weeks of CR. Despite α-diversity reductions after CR, enrichment of Oscillospiraceae spp., solely seen in FXM, correlated with improved psychometric outcomes. This study suggests a promising direction for future interventions targeting mental health through gut microbial modulation.
Lack of Association between Insufficient Intake of Multiple Vitamins and Frailty in Older Adults Who Consume Sufficient Energy and Protein: A Nationwide Cross-Sectional Study
Authors: Park Seung-Guk , Kim Hyoeun
Published: August 2024

Frailty is a complex condition that intensifies with age and is marked by decreased physiological function. We rigorously investigated the effects of lower vitamin intake on frailty using data from 665 adults aged over 65 years who consumed sufficient recommended daily energy and protein intakes from the Korean Nutrition and Health Survey, 2016–2019. The definition of frailty was modified based on Fried et al.’s definition of weight loss, exhaustion, weakness, slowness, and low energy expenditure. Based on daily intake, we analyzed vitamins such as vitamin A, thiamine, riboflavin, niacin, folic acid, and vitamin C. Our results of logistic regression showed that increasing multiple deficiencies in several kinds of vitamins (mild to moderate to severe) is not associated with frailty (odds ratio: 1, 1.24 (0.24–3.10), 0.82 (0.28–2.39), p for trend = 0.626) in older adults who consumed sufficient calories and proteins. A subgroup analysis of age and sex, which may interfere with the relationship between vitamin intake and frailty, showed that vitamin intake was not associated with frailty when sufficient energy and proteins were consumed. Furthermore, there was no difference in the prevalence of frailty between the groups with sufficient and insufficient intakes of individual vitamins.
The Frequency and Predictive Factors of Change in Alcohol Consumption during the COVID-19 Pandemic: Results from a Multi-Country Longitudinal Study
Authors: Verheij Carolien , Alexandrov Nadja , Lubetkin Erica I. , Bonsel Gouke J. , Yfantopoulos John N. , Janssen Mathieu F. , Schuit Stephanie C. E. , Polinder Suzanne , Rood Pleunie P. M. , Haagsma Juanita A.
Published: August 2024

Background: The COVID-19 pandemic has had multiple health and behavioral effects in the general population worldwide, including effects on nutritional and lifestyle behavior such as alcohol consumption. This study aimed to determine the frequency of and predictors for change in alcohol consumption two years after onset of the COVID-19 pandemic among participants from the general population of six countries. Methods: Longitudinal study design with 4999 participants (47% male; aged 18–75 years) from a general population cohort from six countries: Greece, Italy, the Netherlands, Sweden, the United Kingdom (UK) and the United States of America (US). Measurements: Three web-based surveys at different time waves: T1 = 22 April–1 June 2020; T2 = 2 May–29 June 2021 and T3 = 29 April–25 June 2022. The surveys included questions on self-reported retrospective alcohol consumption, demographics, health, anxiety and depression symptoms and recent life events. Results: Of 4999 respondents, most (82.3%) reported no change in drinking habits during the pandemic, whereas 12.5% reported drinking less and 5.1% drinking more. Predictive factors for increased alcohol consumption include age 35–54 years, male gender, high educational level, moderate-severe depression symptoms, excessive drinking before the COVID-19 pandemic, no change in general health status and job loss. Predictive factors for decreased alcohol consumption were age 18–34 years, male gender, having chronic disease(s), moderate-severe depression symptoms, excessive drinking before the pandemic and job loss. Conclusion: The proportion of participants who reported a decrease in alcohol consumption during the COVID-19 pandemic was higher compared to those who reported an increase. Excessive drinking before the pandemic, depression symptoms and job loss were predictors for both drinking more and drinking less alcohol during the COVID-19 pandemic with an stronger association for an increase in alcohol consumption.
Antinociceptive and Anti-Inflammatory Activities of Acetonic Extract from Bougainvillea x buttiana (var. Rose)
Authors: Castañeda-Corral Gabriela , Cedillo-Cortezano Mayra , Aviles-Flores Magdalena , López-Castillo Misael , Acevedo-Fernández Juan José , Petricevich Vera L.
Published: August 2024

Background:Bougainvillea x buttiana is an ornamental plant with antioxidant, anti-inflammatory, and cytotoxic activities, which has been traditionally used to treat respiratory diseases. This study aimed to investigate whether the acetonic extract of Bougainvillea x buttiana var. Rose (BxbRAE-100%) has analgesic and anti-inflammatory properties and its potential action mechanisms. Methods: Analgesic and anti-inflammatory activities were evaluated using three murine pain models and two acute inflammation models. In vitro, the ability of the extract to inhibit proteolytic activity and the activities of the enzymes phospholipase A2 (PLA2) and cyclooxygenase (COX) were evaluated. In silico analysis was performed to predict the physicochemical and Absorption, distribution, metabolism, and excretion (ADME) profiles of the compounds previously identified in BxbRAE-100%. Results: In vivo BxbRAE-100% decreased the nociceptive behaviors in the writhing model, the tail immersion, and the formalin test, suggesting that the extract has the potential to relieve pain at peripheral and central levels. Additionally, topical or oral BxbRAE-100% treatment reduced dose-dependent 12-O-Tetradecanoylphorbol-13-acetate (TPA)-induced ear inflammation and carrageenan-induced paw edema, respectively. In vitro, BxbRAE-100% significantly inhibited proteolytic activity and PLA2, COX-1 and COX-2 activities. In silico, the compounds previously identified in BxbRAE-100% met Lipinski’s rule of five and showed adequate ADME properties. Conclusions: These results support the use of B. x buttiana in Traditional Mexican Medicine and highlight its potential for the development of new treatments for pain and inflammation.
Pharmacokinetic Simulation and Area under the Curve Estimation of Drugs Subject to Enterohepatic Circulation
Authors: Alpízar Melchor , de Jesús Reséndiz José , García Martínez Elisa , Dwivedi Sanyog , Trejo Miguel Alejandro
Published: August 2024

Enterohepatic circulation (EHC) is a complex process where drugs undergo secretion and reabsorption from the intestinal lumen multiple times, resulting in pharmacokinetic profiles with multiple peaks. The impact of EHC on area under the curve (AUC) has been a topic of extensive debate, questioning the suitability of conventional AUC estimation methods. Moreover, a universal model for accurately estimating AUC in EHC scenarios is lacking. To address this gap, we conducted a simulation study evaluating five empirical models under various sampling strategies to assess their performance in AUC estimation. Our results identify the most suitable model for EHC scenarios and underscore the critical role of meal-based sampling strategies in accurate AUC estimation. Additionally, we demonstrate that while the trapezoidal method performs comparably to other models with a large number of samples, alternative models are essential when sample numbers are limited. These findings not only illuminate how EHC influences AUC but also pave the way for the application of empirical models in real-world drug studies.