bluuebunny/crossref_metadata_2025 huggingface dataset:
La Houle (1913-1931) d’Émile Moselly : lecture
Authors: Leroy Christian
Published: December 2022

La Houle (1913-1931), d’Émile Moselly. Cette lecture présente la manière dont l’écrivain réaliste lorrain parle de la vie quotidienne d’une famille de pêcheurs du Boulonnais face aux épreuves de la mer. L’histoire se mue en véritable tragédie du fatum antique quand Maria ne réussit pas à détourner son fils d’aller risquer sa vie sur un chalutier peu fiable. Le romancier se révèle alors un excellent portraitiste d’âme.
De Silex éclaté aux Rives incertaines , Robert Mallet, amoureux de la côte picarde
Authors: Crampon Monique
Published: December 2022

Robert Mallet, né à Paris, de souche picarde, est attaché à la Baie de Somme qui est présente dans ses poèmes et ses romans dès les années 1950. Monique Crampon montre son attachement à la nature et son souci de défendre l’environnement qui l’amèneront à agir administrativement dans les années 1970 pour défendre la Baie de Somme.
Unis contre la faim ? Associations caritatives, pouvoirs publics et industrie dans l’économie politique des banques alimentaires
Authors: Lohnes Joshua , Béja Alice
Published: December 2022

Suite à la délégation de services publics vers des acteurs privés sous la présidence Reagan, l’économie des banques alimentaires aux États-Unis est devenue un marché de plusieurs milliards de dollars. Les mouvements sociaux et politiques qui ont institutionnalisé les réseaux alimentaires caritatifs sont divers et hétérogènes, illustrant les intérêts concurrents d’un système alimentaire américain depuis longtemps aux prises avec des tensions paradoxales entre le gaspillage alimentaire et la faim. Cet article analyse l’économie morale de la lutte contre la faim au sein d’un mouvement social diversifié qui négocie un ensemble de codes juridiques et de normes sociales établis au cours des quarante dernières années. Il fait l’hypothèse que les réseaux caritatifs sont un point d’observation privilégié de la compétition politique autour de l’avenir du système alimentaire américain.
Le travail parental, dernier verrou de la révolution de genre. Une revue de littérature internationale
Authors: Delès Romain
Published: December 2022

Le travail parental apparaît comme le dernier verrou de la révolution de genre. En effet, si le processus d’égalisation entre les femmes et les hommes est engagé depuis plusieurs décennies en matière de travail rémunéré et de travail domestique, le travail parental demeure « protégé » de ces évolutions. En cause : la scène domestique et l’activité parentale sont le lieu de l’accomplissement des normes de genre, comme le démontrent les approches dites du « doing gender ». Le travail parental est alors l’objet de contre-tendances inégalitaires à l’intérieur de la révolution de genre. L’article met en perspective la littérature française et internationale sur le sujet et tente d’expliquer pourquoi le constat d’une « grande convergence » n’est pas incompatible avec la persistance d’inégalités tenaces.
Engagement paternel, santé de la famille et soutien aux pères
Authors: Fusulier Bernard , Scacchitti Cecilia , Mahieu Céline
Published: December 2022

Cette revue de littérature porte sur l’engagement des pères et les relations qu’ils entretiennent avec les professionnel.le.s de la petite enfance. Les pères sont dorénavant reconnus comme des agents importants de la santé de l’enfant et de celle des parents. Ils sont également plus impliqués dans les soins apportés aux enfants, même si les manières d’être père sont plurielles. De leur côté, les intervenant.e.s sont disposé.e.s et incité.e.s à intégrer les pères dans leurs pratiques de soins. Il n’en demeure pas moins que les services restent tendanciellement matricentrés. Force est de reconnaître que les relations entre les pères et les intervenant.e.s sont complexes du fait des systèmes normatifs encastrés dans des logiques sociales, un ordre genré et un ethos professionnel.
Former autrement les futurs enseignants à la relation école-familles. L’expérience sensible d’un atelier d’écriture
Authors: Payet Jean-Paul , Rufin Diane
Published: December 2022

Afin de permettre aux futurs enseignants en formation initiale de développer une réflexivité sur la relation entre l’école et les familles, il apparaît nécessaire d’imaginer un dispositif fondé sur les savoirs de la recherche mais adoptant une pédagogie en rupture avec l’enseignement universitaire conventionnel. L’article présente l’expérimentation d’un atelier d’écriture et analyse les processus – de remémoration, d’identification, d’imagination – par lesquels les étudiants s’engagent affectivement et intellectuellement dans une réflexion sur les relations école-familles. Les activités d’écriture (et de jeux de rôle) tissent une relation à soi et aux autres participants dans un cadre de confiance propice à la réflexivité et à la construction d’un sens critique. Un tel dispositif qui vise à réconcilier professionnalité et sensibilité entend ouvrir les futurs enseignants à la diversité des façons d’être une famille et les invite à prendre en compte le point de vue des parents.
« S’il n’y a pas une transparence totale, il n’y aura pas une confiance totale » – Retour sur les dispositifs de divulgation des liens d’intérêts en santé
Authors: Boullier Henri , Greffion Jérôme
Published: December 2022

Depuis une trentaine d’années, la notion de « conflits d’intérêts » est utilisée pour désigner les liens problématiques entre les entreprises pharmaceutiques et les professionnels de santé. Elle a donné lieu à la création de dispositifs de divulgation (déclarations des liens d’intérêts dans les revues, déclarations publiques d’intérêts pour l’expertise sanitaire) sous la forme de sites internet publics qui inventorient l’intégralité des échanges financiers entre les entreprises pharmaceutiques et les professionnels du secteur. Depuis 2014, le site Transparence-Santé rend toutes ces données accessibles. À partir de travaux en sciences sociales, nous retraçons ici la façon dont l’impératif de transparence des liens d’intérêts s’est matérialisé en France, en rendant compte de la manière dont ce dispositif a été mis en œuvre, en identifiant ses enjeux, ses effets, et certaines de ses limites.
O eclipse do Novo Mundo
Authors: Ziller Camenietzki Carlos
Published: December 2022

Les documents et les réflexions sur les éclipses, réalisés tout au long des premiers siècles de l’occupation européenne des Amériques, révèlent un effort intense pour caractériser les significations du phénomène à l’intention des populations locales et des diverses métropoles du Vieux Monde. De fait, l’intelligentsia américaine a avancé ses interprétations du phénomène dans les mêmes cadres que ceux de ses matrices européennes, qu’elles soient ouvertement ou de manière occulte astrologiques, qu’elles soient plutôt techniques et mathématiques ou, enfin, philosophiques et poétiques.
L’éclipse de Don Quichotte
Authors: Le Bihan Adrien
Published: December 2022

Peu de lecteurs retiennent le dialogue entre don Quichotte et un berger où il est question d’éclipse. Effacement comparable : aucun des observateurs de l’éclipse du soleil de 1900, même dans la Manche où elle est bien visible, ne songe à l’éclipse de Don Quichotte . Celle-ci se voit même éclipsée des Méandres de Léon-Paul Fargue, fin connaisseur de l’Espagne et de l’astrologie, bien qu’il y garde trace de celle de 1900.
Éclipses à l’italienne
Authors: Motoret Laurence
Published: December 2022

Variations sur le thème de l’éclipse chez Antonioni.
Suzanne Martin. Art de l’éclipse et éclipse de l’art
Authors: Roger Christian
Published: December 2022

À la faveur de quelques éclipses remarquables du siècle passé, Suzanne Martin nous fait croiser à son insu Francis Picabia, Odilon Redon, Rainer Maria Rilke, Gérard de Nerval, Saint John-Perse, Jean Paulhan, André Breton et quelques autres, autour de trois romans et d’une importante œuvre picturale, dont une gouache aussi fabuleuse qu’énigmatique.
Les silences de Catherine Guérard
Authors: Buénerd Renaud , Grosso François
Published: December 2022

Catherine Guérard (1929-2010) a publié deux romans de son vivant. Lorsque les éditions du Chemin de fer décident de rééditer le second en 2021, toute trace de l’autrice a disparu. Les éditeurs interrogent cette éclipse, à l’aune des éléments biographiques qu’ils ont pu rassembler : pourquoi, entre 1967, parution de Renata n’importe quoi , et le décès de Catherine Guérard en 2010, ce silence jamais rompu ?
L’expansion internationale de Harlequin Mills & Boon : de la « Bibliothèque coloniale » au marketing dédié au lectorat indien moderne
Authors: H. Sutton Denise , T. Dulot Nadège
Published: December 2022

Cet article explore l’impact en Inde de la « Bibliothèque coloniale » de l’éditeur britannique Mills & Boon (M&B), ainsi que son adaptation aux nouveaux marchés constitués au sein de ce que l’historien des entreprises Geoffrey Jones appelle les Première et Seconde économies mondialisées. À sa création en 1908, M&B s’est lancé dans la distribution d’ouvrages de romance populaire dans les territoires colonisés, créant ainsi auprès du lectorat anglophone de ces territoires une solide image de marque qui se maintient jusqu’à nos jours. Après l’acquisition de M&B par Harlequin en 1972, le groupe Harlequin Mills & Boon (HMB) s’est appuyé sur la popularité de la marque M&B pour accroître sa présence sur le marché indien anglophone. En 2008, le groupe a ouvert un bureau local à Mumbai et proposé des romances populaires écrites par des auteurs indiens pour un lectorat indien. Les stratégies employées par HMB sur ce marché offrent un aperçu des demandes d’une nouvelle génération de lecteurs en Inde et un objet d’étude permettant de mettre au jour les caractéristiques d’une première et d’une seconde économie mondialisée.
The impact of information and communication technology on financial inclusion-based on a global perspective
Authors: Li Zhenghui , Chen Bin , Lu Siting
Published: December 2022

In order to test the effect of information and communication technology (ICT) level on financial inclusion, based on the sample data of countries around the world in 2011, 2014, and 2017, this paper firstly uses the coefficient of variation method and the Euclidean distance method to construct the ICT level index and the financial inclusion index, and then conducts empirical analysis through a linear regression model. Further, a mediating effect model is employed to explore the impact mechanism of the ICT level on financial inclusion. Finally, the impact heterogeneity is explored based on the internal and external characteristics of each country. The empirical results show that: first, the ICT level can effectively improve financial inclusion, but its effects on the width and the depth of financial inclusion are different; second, the ICT level improves a country's financial inclusion by promoting the improvement and development of the digital payment system; third, the impact of the ICT level on financial inclusion shows significant heterogeneity among different countries or regions with differences in banking structure, economic development level and international financial environment. Last but not least, the conclusions of this paper can help countries or regions to improve financial inclusion in a targeted manner through information and communication technology.
Uncertain multi-objective dynamic weapon-target allocation problem based on uncertainty theory
Authors: Li Guangjian , He Guangjun , Zheng Mingfa , Zheng Aoyu
Published: December 2022

The weapon-target allocation (WTA) problem is a fundamental subject of defense-related applications research, and previous studies assume that the parameters in the model are determinate. For the real battlefield, asymmetric information usually leads to the failure of the above assumption, and there are uncertain factors whose frequency is hard to pinpoint. Based on uncertainty theory, we study a WTA problem in indeterminate battlefield in this paper. First, we analyze the uncertain factors in indeterminate battlefield and their influence on WTA problem. Then, considering the target threat value, the protected asset value and the extra cost of interception as uncertain variables, the uncertain multi-objective dynamic WTA (UMDWTA) model is established, where three indices including the value of destruction of targets, the value of surviving assets and the cost of operation are regarded as objective functions, and on this basis, an equivalent transformation is presented to convert the UMDWTA model into a determinate multi-objective programming (MOP) problem by expected value and standard deviation principle. To solve the proposed model efficiently, an improved multi-objective evolutionary algorithm based on decomposition (MOEA/D) is designed, which employs three new evolutionary operators and the weight vectors adaptation mechanism to improve the convergence and uniformity of the Pareto front obtained. Finally, a case of the UMDWTA problem is carried out to be solved by the designed algorithm, and the results verify the feasibility of the proposed model.
Investigating Students' Conceptual Understanding of Socio-Environmental Problems
Authors: Mettis Kadri , Väljataga Terje
Published: December 2022

The aim of this study was to investigate how mobile outdoor learning supports the development of students' conceptual understanding about local socio-environmental problems and students' attitudes towards the learning activity. To explore these aspects, a study following a design-based research model was designed and conducted with 68 eighth-ninth grade students. The learning design of the activity incorporated a number of mobile learning tools: environmental data collecting devices, tablets and students' smartphones with outdoor learning apps to facilitate the learning activity, which was mainly conducted outdoors. Research data was collected with semi structured pre- and post-questionnaires. The results showed that the students' conceptual understanding about the study topic developed significantly, and they were positively minded about their experience in working on a socio-environmental issue. Based on the results, it can be concluded that mobile outdoor learning activities focusing on timely socio-environmental problems have the potential to increase students' conceptual understanding.
The Impact of Government Expenditure on Inflation: Evidence from Sri Lanka and India
Authors: Shifaniya A.J.F. , Hettiarachchi K.H.I.S. , Weeraddana M.N. , Parmila N.S.
Published: December 2022

This study analyses the impact of government expenditure on inflation in Sri Lanka and India from 1977 to 2019, using ARDL Co-integration, Bounds test, Error Correction version of the ARDL model and the Granger Causality test, while employing inflation, government expenditure and interest rates as study variables. Results for both Sri Lanka and India reveal a statistically significant and a positive relationship between government expenditure and inflation in the long run: A 1% increase in government expenditure tends to increase inflation by 0.0793% and 4.6469% for Sri Lanka and India respectively. The coefficient of the Error Correction Term for both countries carry a negative sign and are statistically significant, indicating an adjustment towards equilibrium at a speed of 63.8% and 93.94% respectively, one period after exogenous shocks. Granger causality test indicates a unidirectional causality stemming from government expenditure towards inflation only in the case of Sri Lanka. This highlights Sri Lanka’s need to manage its public expenditure and its impact on money supply in order to achieve price stability. It is advisable for fiscal as well as monetary policy makers to work closely so as to control inflationary pressure on the economy resulting from rising government expenditure.
Acknowledgement to reviewers
Authors: Office Editorial
Published: December 2022

No abstract available.
Administration of L-citrulline prevents Plasmodium growth by inhibiting/ modulating T-regulatory cells during malaria pathogenesis
Authors: Awasthi Vikky , Chauhan Rubika , Das Jyoti
Published: December 2022

Background & objectives: Malaria affects around 228 million people all over the globe. Malaria causing parasite Plasmodium infection leads to activation of immune responses. The growth of parasite and immune activation requires semi essential amino acids like L-arginine. Malaria infection leads to condition of hyperargininemia and low availability of nitric oxide. However, the effect of L-arginine supplementation in malaria infected mice has not been explored in in-vivo studies. In this study we have compared the effect of oral supplementation of nitric oxide donor, L-arginine and L-citrulline, in malaria infected mice

Methods: To examine the effect of oral supplementation of L-arginine and L-citrulline, Plasmodium berghei infected mice were divided in different groups and respective groups were fed with L- arginine and L-citrulline, parasitemia was measured on different days. Mice was sacrificed and immunophenotyping was done on 10 days post infection.

Results: our results show that supplementation of L-arginine induces conducive environment for Plasmodium growth due to which the infected mice dies earlier than control wild type infected mice whereas L-citrulline supplementation inhibits parasite growth and mice survives for longer period of time. Flow cytometric analysis shows that supplementation of L-arginine increases cTLA-4 on T cell population, increases Treg cells leading to immunosuppression while supplementation of L-citrulline does not have effect on T cells population and number of Treg cell decrease compared to P. berghei infected mice.

Interpretation & conclusion: our results show that L-citrulline can be a better alternative than L-arginine because of lower expression of inhibitory molecules and lower parasitemia as well as increased survival of infected mice.
Prevalence of Pfhrp2/3 gene deletions among false negative rapid antigen test results in central India
Authors: Kumari Sarita , Ahmed Md. Zohaib , Sharma Supriya , Pande Veena , Anvikar Anupkumar R
Published: December 2022

Background &objectives: The diagnosis of Plasmodium falciparum malaria is widely dependent on the P. falciparum histidine rich protein 2 (PfHRP2) antigens based rapid diagnostic tests. There are few possible factors like Pfhrp2 polymorphism, Pfhrp2 deletion and density of malaria parasite which can affect the sensitivity of the Pf-HRP2-based RDT. The primary objective of the investigation was to check whether the Pfhrp2 gene deletion is the primary cause of RDT false negative cases.

Methods: Febrile patients from three districts of Chhattisgarh, India were screened for malaria during 2016–2017 by microscopy and RDT. All microscopy P. falciparum positive samples were validated by PCR. Microscopy positive and RDT negative samples were analyzed for the presence of Exon 2, across Exon 1-2, upstream and downstream of both the Pfhrp2 and Pfhrp3 genes fragment by PCR.

Results: Out of 203 screened samples, 85 were detected positive for P. falciparum malaria based on microscopy and PCR. Among these 85 P. falciparum positive samples, 4 samples were observed Pf-HRP2 RDT negative. Although, it signified that the RDTs used were reliable with sensitivity of 95.3% (81/85). 3/4 PfHRP2-RDT negative samples of the P. falciparum isolates exhibited complete deletion of Pfhrp2 and Pfhrp3 genes and one sample was found RDT false negative due to high parasite density.

Interpretation & conclusion: Pfhrp2 and Pfhrp3 deletions that result in false negative RDTs were uncommon in our setting. The continued monitoring of RDTS which results in false negative tests due to Pfhrp2/3 gene deletion is the need of the hour for an effective malaria elimination strategy.
Dengue infection among tribal population in the Nilgiris district, Tamil Nadu, India
Authors: Samuel P Philip , Govindarajan R , Krishnamoorthi R , Leo S Victor Jerald , Rajamannar V , Nagaraj J.
Published: December 2022

Background & objectives: Dengue emerged as an important public health problem in Tamil Nadu from 2000 onwards, reported in all the districts as an endemic disease of Tamil Nadu except Nilgiris district. So this study was carried out to understand the dengue epidemiology in Nilgiris district.

Methods: Block-wise study was made at the Nilgiris district. The clinicians at the Nilgiris Adivasi Welfare Association hospitals (NAWA) situated in Kotagiri, Kozhikarai and Primary Health Centers from Kunjpannai, Arayoor, and Soloor Mattam, examined and recorded symptoms and collected blood samples from the dengue-suspected patients. These samples were centrifuged at 4°C and stored. Serum samples (267 nos.) collected from dengue-suspected patients for two years period from 2014 to 2016 were screened for dengue infection.

Results: First year study conducted during 2014-15 showed 13 dengue positives (8.39%) mainly from Kotagiri block (9 nos. - 69.2%) and the second year study conducted during 2015-16 showed 12 dengue positives (10.71%) found mostly from Udagamandalam block (6 nos.- 50%). People belonging to 6 different tribes - Irular, Toda, Kota, Kurumba, Kattunaickan, and Paniya were found infected with dengue and more Irular positives were recorded in both the years (5 Irular-2014-15 & 11 Irular -2015-16). First year detected more female positives (92.3%) whereas the second year showed 5 males (41.7%) and 7 females (58.3%).

Interpretation & conclusion: This study unearthed the hidden disease dengue to be prevalent among the tribal community and emphasized the need for the establishment of a permanent dengue surveillance system with improved disease diagnostics, to initiate effective vector control efforts to stop dengue transmission from this hilly region.
Durability, household usage and washing pattern of DuraNet© and Interceptor® long-lasting insecticidal nets in long-term field trials in India
Authors: Sharma SK , Yadav RS , Srivastava HC , Bhatt RM , Pant CS , Haque MA , Sreehari U , Raghavendra K.
Published: December 2022

Background & objectives: Universal coverage of population with long-lasting insecticidal nets (LLINs) living in endemic areas inhabited by ethnic tribal communities or in difficult to reach areas was found effective for disease control where other interventions such as indoor residual spraying have limited success because of operational issues. Evaluation of different LLINs with varied insecticides and fabrics are being evaluated to meet the demand of new products. This study was undertaken on two brands of LLINs, DuraNet© and Interceptor® that varied in fabric and manufacturing technologies to assess the usability in field conditions for atleast three years.

Methods: In large-scale field trials DuraNet©, alpha cypermethrin incorporated polyethylene net, was evaluated in Odisha state while, Interceptor® net, alpha cypermethrin coated polyester net was evaluated in Chhattisgarh and Gujarat states for a period of three years following WHO guidelines. Durability, usage pattern and washing behavior were monitored through periodic surveys and physical examination of nets.

Results: Survivorship of both the nets was 84–100% and every night usage rate was >62% in all seasons. Washing frequency was largely within the prescribed limits. The proportion of DuraNet© with holes was 26.7% in year one 74% in year three. In Gujarat, proportion of Interceptor® nets with holes at six months was 33.3% increased to 87% in year three and in Chhattisgarh, 6.7% after six months to 93.3% after three years of use respectively.

Interpretation & conclusion: Both the nets revealed a useful life of three years under the field condition
Validation of micro-chip based PCR assays for diagnosis of both Plasmodium falciparum and Plasmodium vivax
Authors: Hussian Syed Shah Areeb , Ojha Vijay P. , Jeena Meenakshi , Rahi Manju , Anvikar Anupkumar , Sharma Amit , Dhiman Ramesh C.
Published: December 2022

Background & objectives: Microscopy is considered as the gold standard for malaria diagnosis, however sub-microscopic infections can only be detected by Polymerase chain reaction, which demands high cost and elaborate laboratory setup. The Micro-chip PCR based Truenat Malaria Pv-Pf and Pf assay is a portable solution for detection of sub-microscopic/asymptomatic cases of malaria in the field, three lots of which were evaluated for P. falciparum and P. vivax malaria.

Methods: Three lots of Truenat® Malaria Pv-Pf and Pf assay (kits) were assessed using blood samples of P. vivax and P. falciparum as well as malaria negative blood samples. DNA was extracted from the blood samples using the Trueprep Auto v2 Universal Cartridge based sample prep device and real time qPCR was performed using Truelab DUO micro PCR Analyzer with three lots of Truenat® Malaria Pv-Pf and Pf Assays. Mean, Standard deviation and one-way analysis of variance (ANOVA) was used to assess the significance of inter-lot variability in Cycle threshold values.

Results: The Truenat® Malaria Pv-Pf and Pf assays identified the malaria parasites with 100% accuracy. Based on the test for variance (ANOVA) the inter-lot variability in cycle threshold values were not significant, indicating a high degree of precision.

Interpretation & conclusion: Based on high accuracy and precision between different lots, the Truenat® Malaria Pv-Pf and Pf assays were found to be suitable for the diagnosis of sub-microscopic infections in field conditions to provide support in elimination of malaria.
Effectiveness of One Health approach for control of Kyasanur Forest Disease in Wayanad, Kerala, India
Authors: Prejit  , Hitziger M , Asha K
Published: December 2022

Background & objectives: Kyasanur Forest Disease (KFD) is a vector borne haemorrhagic fever that is endemic in the Wayanad region located in Northern part of Kerala, India. The region is managing the outbreak well ever since the major epidemic of 2015. This was because of the successful implementation of One Health (OH) initiative concentrating on multisectoral collaboration between regional institutions involved in public, animal and environmental health domains. The article presents how OH was implemented for the first time in the district in the year 2015 and evaluates the degree OH-ness of the Initiative.

Methods: The OH approach involved trans-disciplinary stakeholder meetings and reviews, outbreak management and integrated surveillance targeting ticks, monkeys and humans. The degree of OH-ness used for addressing KFD during the year 2015 was evaluated following the protocol developed by the Network for Evaluation of One Health (NEOH). In detail, we (i) described the OH initiative and its system (Aim, stakeholders, action strategy) and (ii) scored different aspects of this initiative (i.e., OH-thinking, -planning, -working, -sharing, -learning, -organization), with values from 0 (=no OH approach) to 1 (=perfect OH approach).

Results: We obtained a median score for each aspect evaluated. We reached high scores for OH systemic organization (1.0), OH thinking (0.83) and OH working (0.83). Lower scores were attributed to OH planning (0.58), OH sharing (0.50) and OH learning (0.33). The OH index was 0.36 and OH ratio was 0.95, indicating a balance between the OH operations and supporting infrastructures.

Interpretation & conclusion: With this we could high-light some critical issues related to communication on sharing data as well as learning gaps for consideration to control future outbreaks. The strengths and weaknesses detected may be used to refine the initiative, aiming to provide a basis for the development of shared recommendations in a more OH-oriented perspective. This model of evaluation criteria will serve to create a database of OH success stories in India that will in turn help to institutionalize the approach at ministerial level. Future India is moving towards implementing a One Health, hence, this study data will provide an ideal opportunity for all sectors to control any vector borne diseases.
Kyasanur forest disease and climatic attributes in India
Authors: Singh Poonam , Kumar Prakash , Dhiman Ramesh C
Published: December 2022

Background & objectives:In India, Kyasanur Forest Disease has been reported from the states of Karnataka, Kerala, Goa, and Maharashtra. The relationship between climatic factors and transmission of KFD remains untouched, therefore, the present study was undertaken.

Methods:Based on the occurrence of cases, Shivamogga district (Karnataka) and Wayanad district in Kerala and northern Goa (Goa state) were selected for the study. Data on the incidence of KFD and climate factors were collected from concerned authorities. To determine the relationship between dependent and independent variables, spearman’s correlation was calculated for monthly as well as with lag months.

Results:KFD cases and temperature (°C) were found significantly correlated up to 1 months’ lag period (p<0.05) while with precipitation relationship was found negatively significant for 0-3 months’ lag. The range of suitable temperature for KFD in Shivamogga, Goa and Wayanad was found as 20-31°C, 25-29°C and 27-31°C respectively. The cumulative precipitation during transmission months (November–May) ranged from <150-500mm, while in non-transmission months (June-October) from >1100-2400mm.

Interpretation & conclusion:The analysis of three sites revealed that with the increase in temperature, the intensity of KFD transmission decreases as corroborated by the seasonal fluctuations in Shivamogga, Goa and Wayanad. High precipitation from June to October rovides suitable ecology to tick vector and sets in transmission season from November to May when cumulative precipitation is <500 mm.
Multiplex loop mediated isothermal amplification (m-LAMP) as a point of care technique for diagnosis of malaria
Authors: Sharma Supriya , Singh Jaskirat , Sen Aparajita , Anvikar Anupkumar R
Published: December 2022

Diagnosis of malaria is a prominent challenge due to the endemic nature of infection. Malaria poses a great threat to global public health. The disease can be diagnosed by several techniques out of which microscopy is a known gold standard. High sensitivity of molecular techniques is making them more reliable and popular as tools for diagnosis of malaria. However, new methods are required which can fulfill the criteria of being Point of Care Test (POCT) as defined by WHO. Loop-mediated isothermal amplification (LAMP) technique amplifies DNA in an isothermal condition, and surpasses the disadvantages of conventional molecular techniques such as polymerase chain reaction. Multiplex LAMP, a modification of LAMP may emerge as a new POC for malaria diagnosis. This review deals with the use of LAMP and multiplex LAMP in diagnosis of malaria and its prospective use as point of care techniques.
Morphological and molecular characterization of Aedes aegypti variant collected from Tamil Nadu, India
Authors: Kumar Nirmal P , Kalimuthu M , Kumar M Senthil , Govindrajan R , Venkatesh A , Paramasivan R , Kumar Ashwani , Gupta Bhavna
Published: December 2022

Background & objectives: Accurate mosquito species identification is the basis of entomological surveys and effective vector control. Mosquito identification is either done morphologically using diagnostic features mentioned in taxonomic keys or by molecular methods using cytochrome oxidase subunit 1 (coxI) and Internal transcribed spacer 2 (ITS2).

Methods: We performed a larval survey for Aedes mosquitoes from eight different geographical regions in Tamil Nadu, India. The mosquitoes collected during the survey were characterized using both morphological and molecular markers.

Results: During an entomological survey from eight different geographical regions in Southern India, a morphological variety named Aedes aegypti var. luciensis was observed. The variant mosquitoes were characterized using both morphological and molecular markers. The variant mosquitoes differed only in the dark scaling of 5th segment of hind-tarsi. Around one third to two third of the 5th segment in variant mosquitoes was dark which has been described as white in identification keys. No other significant difference was observed in adults or immature stages. The variation was heritable and coexisting in the field with the type form mosquitoes. Comparison of the genetic profile of coxI and ITS2 were similar in variant and the type form indicating both of them to be conspecific.

Interpretation & conclusion: The morphological variant mosquitoes were found genetically similar to the Ae. aegypti type form. However, considering its high prevalence and coexistence with Ae. aegypti type form in different geographical regions, detailed studies on bionomics, ecology, genetics, behavior as well as its plausible role in disease transmission are warranted.
Corneal ectasia after an incomplete flap creation in an abandoned laser-assisted in situ keratomileusis
Authors: Tung Hsiao-Fan , Chen Ho-Ling , Liu Yu-Ling , Lin Chang-Ping
Published: December 2022

To report a unique case of a 31-year-old woman developing corneal ectasia after an abandoned laser-assisted in situ keratomileusis (LASIK) procedure with incomplete flap creation without laser ablation. A 31-year-old Taiwanese woman presented with corneal ectasia after a failed LASIK with an incomplete flap creation without laser procedure in her right eye 4 years ago. A visible scar was observed in the flap margin from the 7 to 10 o'clock position. The auto refractometer revealed myopia with high astigmatism, −1.25/−7.25 × 30. Keratometry was 47.00/40.75 D. In contrast, no sign of keratoconus was found in the fellow eye, which did not experience any surgery. Corneal tomography indicated that the incomplete flap scar was compatible with the main area of corneal ectasia. Furthermore, anterior segment optical coherence tomography showed a deep cutting plane and a relatively thin corneal bed. Both findings explained the cause for corneal ectasia. Corneal ectasia can occur whenever corneal structure or integrity is compromised.
Trabecular microbypass stent combined with phacoemulsification in patients with open-angle glaucoma, 1-year outcome in a Taiwanese population
Authors: Su Wei-Wen
Published: December 2022

PURPOSE:

The purpose of this study was to evaluate the safety and efficacy of one trabecular microbypass stent (first generation iStent) combined with phacoemulsification cataract surgery (phaco) in eyes with open-angle glaucoma (OAG) in a Taiwanese population.

MATERIALS AND METHODS:

One trabecular microbypass stent was implanted after phacoemulsification cataract surgery by one surgeon. Intraocular pressure (IOP), medications, corrected distance visual acuity, complications, and adverse events were evaluated.

RESULTS:

This case series included 28 eyes of 25 patients with OAG (19 primary open-angle glaucoma [POAG] and 9 normal tension glaucoma [NTG]). None had previous trabeculectomy or laser trabeculoplasty. At 1 year, the mean IOP decreased from 19.34 ± 3.92 mmHg to 15.84 ± 3.05 mmHg, and the mean number of glaucoma medications decreased from 1.82 ± 1.12 to 0.79 ± 1.17 (both P < 0.001). Longer survival was observed in the NTG eyes (NTG: 11.67 ± 1.00 months, POAG: 9.53 ± 3.78 months, P = 0.031). iStent reposition was performed in one eye, and four eyes received laser treatment for stent occlusion. None had secondary glaucoma surgery.

CONCLUSION:

Combined phacoemulsification and iStent implantations are a safe and effective way in reducing IOP and medication burden in patients with OAG, especially NTG.
COenzyme Q10 ameliorates bisphinol A induced reproductive male toxicity
Authors: Barghash Samia S.M. , Farrag Islam M. , Abd El-Hakam Fatma El-Zahraa , Ali Somia M.M. , Aly Eman M.A.
Published: December 2022

Background and aim

The endocrine disruptor compound bisphenol A (BPA) affects spermatogenesis and exacerbates benign prostate hyperplasia induced by testosterone. Nonetheless, the direct effect of BPA on prostate and testicular cells is not fully investigated. The objective of this study was to evaluate the pathogenic effects of BPA on the structure of the prostate and the ultrastructure of the testis of adult male albino rats via immunohistochemical and transmission electron microscopic study and the potential protective effect of CoQ10 supplementation.

Methods

A total of 30 male albino Wistar rats were categorized into five equal cohorts: group I, no treatment; group II was administered corn oil; group III received coenzyme Q10 (CoQ10); group IV was administrated BPA; and group V received BPA+CoQ10.

Results

BPA administration significantly decreased the mean values of the plasma fertility hormones and serum antioxidant enzymes and increased malondialdehyde. BPA administration markedly affected seminal parameters. Coadministered CoQ10 significantly reversed these biochemical changes. BPA induced histopathological alterations in the epithelium and connective tissue of prostate. Immunohistochemistry of the prostate revealed decreased E-cadherin and increased vimentin expressions in BPA-treated group. Ultrastructural analysis of the testis showed impairment of the basal lamina of seminiferous tubules and tight junctions between Sertoli cells after BPA exposure.

Conclusion

The biochemical and histopathological results of this study revealed direct evidence for BPA-induced male reproductive toxicity in the testes and prostate, causing male infertility. CoQ10 coadministration with BPA partially protects against its damaging effect mediated via its antioxidant capabilities.
The Efficacy of Flexible Ureterorenoscopy Compared with Extracorporeal Shock Wave Lithotripsy for <20 mm Renal Stone: A Systematic Review and Meta-Analysis
Authors: Jamhari Muhammad Arif , Santoso Anugrah Dianfitriani , Soebadi Mohammad Ayodhia
Published: December 2022

Introduction:

Renal stone is a common urological problem that has increased over the years. Extracorporeal shock wave lithotripsy (ESWL) is a noninvasive treatment for patients with <20 mm renal stones, but it also has a lower stone-free rate (SFR) and a higher re-treatment rate (RR) compared to other modalities. On the other hand, flexible ureterorenoscopy (F-URS) can be used to manage larger renal stones and is associated with higher SFR. Thus, we created this study to assess the efficacy and safety of F-URS versus ESWL for patients with <20 mm renal stones.

Methods:

This systematic review and meta-analysis study followed the Preferred Reporting Items for Systematic Reviews and Meta-Analyses guideline and used PICO analysis to systematically search the included studies from several databases, such as PubMed and ScienceDirect. Sixteen relevant studies were included for qualitative and quantitative analysis. Cochrane risk-of-bias tool for randomized trials (RoB) 2.0 was used as bias analysis for RCT studies and the Newcastle-Ottawa Scale for observational studies.

Results:

A total of 2487 patients were included in this study. The SFR was significantly higher in the F-URS group (P < 0.00001), while the auxiliary procedure was significantly higher in the ESWL group (P < 0.00001). However, neither significant difference was observed in the mean operative time, RR Clavien–Dindo classification, and complication rate-based symptom outcomes in both the groups.

Conclusion:

F-URS and ESWL are safe and effective in treating <20 mm renal stones. In terms of SFR, F-URS is superior compared to ESWL. However, a lower complication rate was observed in ESWL.
The Positive Relationship of Body Mass Index, Drain Production, and Tumor Necrosis Factor-alpha in Stage IIIB Breast Cancer Patients after Modified Radical Mastectomy
Authors: Wisnubroto Justinus Dwi Pratjojo , Sunanto Ester Hans
Published: December 2022

Introduction:

Modified radical mastectomy (MRM) is one of the treatments for breast cancer (BC). Seroma is a complication that is quite common and can prolong the length of a patient’s stay after MRM which can measure by drain production. Obesity is known to correlate with drain production and the levels of tumor necrosis factor-alpha (TNF-α). However, the study on the relationship between obesity with drain production and TNF-α remains unclear.

Methods:

This study was an observational analytic design with a cross-sectional approach. Samples consist of third-stage BC patients after MRM in Saiful Anwar Malang Hospital from June 2020 to September 2020. The blood sample will be taken 24 h after MRM and the value of TNF-α will be measured and drain production will be recorded. The statistical analysis using regression test with a significant level of P < 0.05.

Results:

There were 30 patients in this study with a mean age of 47 ± 4.5 years old. The mean height, weight, and body mass index (BMI) were 59 ± 6.7 kg; 156.90 ± 5.8 cm, and 24.75 ± 4.5 kg/m2, respectively. The most BC type was luminal B (60%). Increasing BMI affects drain production with a contribution of 83%, and 17% from another variable. Increasing BMI also affects TNF-α with a contribution of 45%, and 55% from another variable.

Conclusion:

BMI is one of the factors that affects positively drain production and TNF-α in overweight patients after mastectomy than normoweight patients.
Comparative Study between Helicobacter pylori East-Asian-Type with 39-bp Deletions
Authors: Sugiarto Faizah , Nurrosyida Kartika , Wibowo Idznika Nurannisa , Lutvyani Alif , Taufik Dhia Farhan , Alfaray Ricky Indra , Batsaikhan Saruuljavkhlan , Fauzia Kartika Afrida , Dewayani Astri , Miftahussurur Muhammad
Published: December 2022

Introduction:

CagA, an oncogenic virulence factor of Helicobacter pylori, may present with 39-bp deletions in the preepiya region of East-Asian strains resulting in a virulent genotype. This study aims to compare the morphological changes, protein expressions, and interleukin-8 (IL-8) production in AGS cells infected with H. pylori East-Asian strains containing 39-bp deletion.

Methods:

Cultured AGS cells, infected with H. pylori strains obtained from gastric biopsy, were incubated for 24 h (MOI 50, 37°C, 5% CO2). The subsequent changes in cell appearances (Hummingbird phenotype and cell death), protein expressions (CagA, Py99, UreB, and β-actin), and IL-8 production were analyzed through microscopic observation, western blot analysis, and sandwich enzyme-linked immunosorbent assay, respectively.

Results:

Two H. pylori strains (KPG15 and TN2) and negative H. pylori infection negative control (NC) were observed. Cell death was observable in all three groups. Hummingbird phenotype, inducible by CagA, was present in 39-deletion strains. KPG15 and TN2 exhibited positive bands in UreB, CagA, and Py99-confirming H. pylori presence, CagA expression and phosphorylation, respectively. The higher IL-8 concentrations in KPG15 (820.6 pg/ml) compared to TN2 (742.5 pg/ml) and NC (37.1 pg/ml) may be attributable to CagA-independent IL-8 production. These results support the fact that 39-bp deletion induces more severe inflammation.

Conclusions:

KPG15 and TN2 displayed similar in silico profiles, cell appearances, and protein expressions. Although both cases induced IL-8 production, KPG15 had a higher result.
How is Patient Safety Culture, Perceived Organizational Support, and Contextual Performance Impacts Adverse Events?
Authors: Yuniati Dyah , Trisnawati Annis Dwi , Rochmah Thinni Nurul
Published: December 2022

Introduction:

Every health-care facility, including hospitals, has an obligation to prevent harm, misdiagnose, and adverse events for the patient’s receiving healthcare. According to the data, a private hospital in east java has shown a significant increase (24%) in patient adverse events from 2017 to 2019. This study aims to analyze the relationship between patient safety culture and perceived organizational support for adverse events by using contextual performance.

Methods:

An observational analysis with a cross-sectional approach was applied in this study. To determine the sample, we used a random sampling technique and recruited 24 work units from the medical service and support department. Analyzing the relationship between variables has been done using linear regression analysis. The significant data were measured by the P = 0.05. All statistical performance was done utilizing the Statistical Package for the Social Science (SPSS).

Results:

The findings showed that the patient safety culture (b = 0.943) and perceived organizational support (b = 0.951) had an influence on contextual performance (P = 0.000). Whereas patient safety culture has a negative influence on patient safety incidents (P = 0.0002, b = −0.588). While perceived organizational support (P = 0.0012, b = −0.588) and contextual performance (P = 0.004, b = −0.561) have a negative influence on patient safety incidents.

Conclusions:

This study finds that the higher contextual performance, patient safety culture, and perceived organizational support are, the lower the incidence of patient safety will be. Therefore, every hospital should be aware of these three fields to increase the quality of health-care service and prevents adverse events in the patients.
The ocular surface manifestation in ICU coronavirus disease-infected patients
Authors: Elashri Mohammed , Sabry Ibrahim , Elhoseny Mahmoud , Emara Emad , Taha Diaa-Eldin , Sadek Nashwa Shaaban
Published: December 2022

Objectives

Evaluation of the incidence and nature of ocular surface involvement in ICU coronavirus disease (COVID)-infected patients.

Patients and methods

A prospective observational comparative study was carried out between March 2020 and April 2021 on ICU-admitted COVID-19-infected patients and non-COVID patients above the age of 16 years who were admitted to the ICU and met the inclusion criteria.

Results

Of all, 1620 patients were enrolled; 590 were in COVID group, whereas 1030 patients were in non-COVID group. The mean age was 55±22 years. The mean ICU stay was 11.55±4.22 and 8.45±3.93 days in the COVID and non-COVID groups, respectively. Ocular surface manifestations were significantly higher in patients with longer ICU stay (P=0.03). Regarding ocular surface manifestations, eye discharge was the highest incidence in the COVID group, whereas corneal ulcer was the highest in the non-COVID group. There was a significantly higher incidence of ocular surface manifestations in the non-COVID group (P=0.06). Longer ICU stays and low conscious level were significantly associated with more eye manifestations (P=0.03).

Conclusion

COVID-infected patients had the highest rate of eye discharge, whereas non-COVID patients had the highest rate of corneal ulcer. Longer ICU stays and low conscious level were associated with more eye manifestations. COVID-infected patients have no distinguishing characteristics from non-COVID individuals regarding ocular surface signs.
Modified evisceration with posteriorly detached petals for management of deficient sclera
Authors: Salama Omar H. , Alkady Ahmed M.M. , Tharwat Ahmed
Published: December 2022

Aim

The aim of this work was to evaluate the effectiveness of modified evisceration technique compared with standard technique in cases associated with deficient sclera.

Patients and methods

A prospective interventional study was conducted that included 34 eyes of 31 patients with deficient sclera scheduled for modified evisceration technique with intraconal placement of nonporous acrylic implants. Outcome was assessed in terms of implant extrusion, exposure, as well as other early or late postoperative complications.

Results

Adequate volume replacement was achieved in 32 (94.12%) of 34 eyes. Overall, two (5.88%) eyes had mild upper sulcus deficit, and three (8.8%) eyes had mild to moderate prominence of the prosthesis compared with the fellow eye, but no true exposure, extrusion, migration, socket contracture, or sympathetic ophthalmia was encountered. All of the patients were satisfied with the cosmetic and functional outcomes of the procedure, except for the two (5.88%) cases with upper sulcus deficit.

Conclusion

A modified evisceration technique for deficient sclera provides superior cosmetic and functional results compared with the standard technique. It has the advantage of allowing placement of relatively large orbital implants in cases associated with insufficient sclera with very low rates of implant extrusion, exposure, migration, or socket contracture.