bluuebunny/crossref_metadata_2025 huggingface dataset:
High Iodine Induces the Proliferation of Papillary and Anaplastic Thyroid Cancer Cells via AKT/Wee1/CDK1 Axis
Authors: Lv Chunpeng , Gao Yanhui , Yao Jinyin , Li Yan , Lou Qun , Zhang Meichen , Tian Qiushi , Yang Yanmei , Sun Dianjun
Published: March 2021

High iodine can alter the proliferative activity of thyroid cancer cells, but the underlying mechanism has not been fully elucidated. Here, the role of high iodine in the proliferation of thyroid cancer cells was studied. In this study, we demonstrated that high iodine induced the proliferation of BCPAP and 8305C cells via accelerating cell cycle progression. The transcriptome analysis showed that there were 295 differentially expressed genes (DEGs) in BCPAP and 8305C cells induced by high iodine, among which CDK1 expression associated with the proliferation of thyroid cancer cells induced by high iodine. Moreover, the western blot analysis revealed that cells exposed to high iodine enhanced the phosphorylation activation of AKT and the expression of phospho-Wee1 (Ser642), while decreasing the expression of phospho-CDK1 (Tyr15). Importantly, the inhibition of AKT phosphorylation revered the expression of CDK1 induced by high iodine and arrested the cell cycle in the G1 phase, decreasing the proliferation of thyroid cancer cells induced by high iodine. Taken together, these findings suggested that high iodine induced the proliferation of thyroid cancer cells through AKT-mediated Wee1/CDK1 axis, which provided new insights into the regulation of proliferation of thyroid cancer cells by iodine.
Triggered Drug Release From Liposomes: Exploiting the Outer and Inner Tumor Environment
Authors: Franco Marina Santiago , Gomes Eliza Rocha , Roque Marjorie Coimbra , Oliveira Mônica Cristina
Published: March 2021

Since more than 40 years liposomes have being extensively studied for their potential as carriers of anticancer drugs. The basic principle behind their use for cancer treatment consists on the idea that they can take advantage of the leaky vasculature and poor lymphatic drainage present at the tumor tissue, passively accumulating in this region. Aiming to further improve their efficacy, different strategies have been employed such as PEGlation, which enables longer circulation times, or the attachment of ligands to liposomal surface for active targeting of cancer cells. A great challenge for drug delivery to cancer treatment now, is the possibility to trigger release from nanosystems at the tumor site, providing efficacious levels of drug in the tumor. Different strategies have been proposed to exploit the outer and inner tumor environment for triggering drug release from liposomes and are the focus of this review.
Co-Investment Network of ERC-20 Tokens: Network Structure Versus Market Performance
Authors: Liu Si-Hao , Liu Xiao Fan
Published: March 2021

Cryptocurrencies have attracted extensive attention from individual and institutional investors in recent years. In this emerging and inefficient capital market, the roles that institutional investors play can have a remarkable impact on the market. This paper investigates the ERC-20 token investment market from a network perspective. Using a dataset containing 317 ERC-20 tokens and their institutional investors at the end of June 2020, we construct a co-investment network of tokens connected by the sharing of institutional investors. Specifically, we examine whether the tokens’ market embeddedness, measured by their network structural properties, can influence their market performance, as well as whether the tokens’ structural similarity in the co-investment network can influence similarity of their market performance. Our results indicate that strength centrality, closeness centrality, betweenness centrality, and clustering coefficient have a significant impact on trading volume and liquidity of the market. And there is a significantly positive correlation between the Jaccard similarity index and tokens’ market performance similarity. This work demonstrates the non-negligible influence of the institutional investors and the diffusion of such influence through co-investment relationships in the cryptocurrency market. We expect the analysis could further enhance the understanding of the inefficiency and vulnerability of this emerging market.
Temperature Effects During a Sublethal Chronic Metal Mixture Exposure on Common Carp (Cyprinus carpio)
Authors: Castaldo Giovanni , Pillet Marion , Ameryckx Leen , Bervoets Lieven , Town Raewyn M. , Blust Ronny , De Boeck Gudrun
Published: March 2021

The aquatic environment is the final sink of various pollutants including metals, which can pose a threat for aquatic organisms. Waterborne metal mixture toxicity might be influenced by environmental parameters such as the temperature. In the present study, common carp were exposed for 27 days to a ternary metal mixture of Cu, Zn, and Cd at two different temperatures, 10 and 20°C. The exposure concentrations represent 10% of the 96 h-LC50(concentration lethal for the 50% of the population in 96 h) for each metal (nominal metal concentrations of Cu: 0.08 μM; Cd: 0.02 μM and Zn: 3 μM). Metal bioaccumulation and toxicity as well as changes in the gene expression of enzymes responsible for ionoregulation and induction of defensive responses were investigated. Furthermore the hepatosomatic index and condition factor were measured as crude indication of overall health and energy reserves. The obtained results showed a rapid Cu and Cd increase in the gills at both temperatures. Cadmium accumulation was higher at 20°C compared to 10°C, whereas Cu and Zn accumulation was not, suggesting that at 20°C, fish had more efficient depuration processes for Cu and Zn. Electrolyte (Ca, Mg, Na, and K) levels were analyzed in different tissues (gills, liver, brain, muscle) and in the remaining carcasses. However, no major electrolyte losses were observed. The toxic effect of the trace metal ion mixture on major ion uptake mechanisms may have been compensated by ion uptake from the food. Finally, the metal exposure triggered the upregulation of the metallothionein gene in the gills as defensive response for the organism. These results, show the ability of common carp to cope with these metal levels, at least under the condition used in this experiment.
Effects of Climate, Plant Height, and Evolutionary Age on Geographical Patterns of Fruit Type
Authors: Lyu Tong , Wang Yunyun , Luo Ao , Li Yaoqi , Peng Shijia , Cai Hongyu , Zeng Hui , Wang Zhiheng
Published: March 2021

Fruit type is a key reproductive trait associated with plant evolution and adaptation. However, large-scale geographical patterns in fruit type composition and the mechanisms driving these patterns remain to be established. Contemporary environment, plant functional traits and evolutionary age may all influence fruit type composition, while their relative importance remains unclear. Here, using data on fruit types, plant height and distributions of 28,222 (∼ 90.1%) angiosperm species in China, we analyzed the geographical patterns in the proportion of fleshy-fruited species for all angiosperms, trees, shrubs, and herbaceous species separately, and compared the relative effects of contemporary climate, ecosystem primary productivity, plant height, and evolutionary age on these patterns. We found that the proportion of fleshy-fruited species per grid cell for all species and different growth forms all showed significant latitudinal patterns, being the highest in southeastern China. Mean plant height per grid cell and actual evapotranspiration (AET) representing ecosystem primary productivity were the strongest drivers of geographical variations in the proportion of fleshy-fruited species, but their relative importance varied between growth forms. From herbaceous species to shrubs and trees, the relative effects of mean plant height decreased. Mean genus age had significant yet consistently weaker effects on proportion of fleshy-fruited species than mean plant height and AET, and environmental temperature and precipitation contributed to those of only trees and shrubs. These results suggest that biotic and environmental factors and evolutionary age of floras jointly shape the pattern in proportion of fleshy-fruited species, and improve our understanding of the mechanisms underlying geographical variations in fruit type composition. Our study also demonstrates the need of integrating multiple biotic and abiotic factors to fully understand the drivers of large-scale patterns of plant reproductive traits.
Compliance and Self-Reporting During the COVID-19 Pandemic: A Cross-Cultural Study of Trust and Self-Conscious Emotions in the United States, Italy, and South Korea
Authors: Travaglino Giovanni A. , Moon Chanki
Published: March 2021

The coronavirus COVID-19 pandemic is an unprecedented health crisis. Many governments around the world have responded by implementing lockdown measures of various degrees of intensity. To be effective, these measures must rely on citizens’ cooperation. In the present study, we drew samples from the United States (N= 597), Italy (N= 606), and South Korea (N= 693) and examined predictors of compliance with social distancing and intentions to report the infection to both authorities and acquaintances. Data were collected between April 6th and 8th 2020. We investigated the role of cultural orientations of horizontal and vertical individualism and collectivism, self-conscious emotions of shame and guilt related to the infection and trust in the government’s action. Across all countries, vertical collectivism (VC) predicted stronger shame, whereas horizontal collectivism predicted stronger trust in the government. Only in the United States, VC was associated with stronger trust. Stronger feelings of shame predicted lower compliance and intentions to report the infection to both authorities and acquaintances. In contrast, guilt was associated with stronger intentions to report the infection to the authorities. Finally, trust was associated with stronger compliance and intentions to report the infection to the authorities. Unlike Italy and South Korea, the association between trust on compliance was not statistically significant in the United States, implications of the findings, and directions for future research are discussed.
An Exploration of Mental Health Discussions in Live Streaming Gaming Communities
Authors: Gandhi Reesha , Cook Christine L. , LaMastra Nina , Uttarapong Jirassaya , Wohn Donghee Yvette
Published: March 2021

Live streaming is a unique form of media that creates a direct line of interaction between streamers and viewers. While previous research has explored the social motivations of those who stream and watch streams in the gaming community, there is a lack of research that investigates intimate self-disclosure in this context, such as discussing sensitive topics like mental health on platforms such as Twitch.tv. This study aims to explore discussions about mental health in gaming live streams to better understand how people perceive discussions of mental health in this new media context. The context of live streaming is particularly interesting as it facilitates social interactions that are masspersonal in nature: the streamer broadcasts to a larger, mostly unknown audience, but can also interact in a personal way with viewers. In this study, we interviewed Twitch viewers about the streamers they view, how and to what extent they discuss mental health on their channels in relation to gaming, how other viewers reacted to these discussions, and what they think about live streams, gaming-focused or otherwise, as a medium for mental health discussions. Through these interviews, our team was able to establish a baseline of user perception of mental health in gaming communities on Twitch that extends our understanding of how social media and live streaming can be used for mental health conversations. Our first research question unraveled that mental health discussions happen in a variety of ways on Twitch, including during gaming streams, Just Chatting talks, and through the stream chat. Our second research question showed that streamers handle mental health conversations on their channels in a variety of ways. These depend on how they have built their channel, which subsequently impacts how viewers perceive mental health. Lastly, we learned that viewers’ reactions to mental health discussions depend on their motivations for watching the stream such as learning about the game, being entertained, and more. We found that more discussions about mental health on Twitch led to some viewers being more cautious when talking about mental health to show understanding.
Health Seeking Behavior Among Adults and Elderly With Chronic Health Condition(s) in Albania
Authors: Gabrani Jonila , Schindler Christian , Wyss Kaspar
Published: March 2021

Aim: Assess the use of different health care service providers by adults (aged 18–59) and elderly (aged > =60) who suffer from non-communicable disease (NCD) and explore relationships between sociodemographic variables and care-seeking behaviors.

Methods: A cross-sectional survey was conducted in the districts of Diber and Fier in December 2018, using random cluster sampling. Descriptive statistics were used to compare the care-seeking behaviors of adults and elderly people. We employed binary and multinomial logistic regression to assess factors associated with the type of health service provider used. Analyses were adjusted for clustering within districts of residence.

Results: Out of 3,799 respondents, 1,116 (29.4%) suffered from an NCD. Of these, 95% sought to obtain care for their chronic condition through public healthcare providers. The elderly were more likely to use primary healthcare services (PHC) to initiate care when facing health problems (56%), compared to those aged 18–59 years (49%, p < 0,001). Over the last 8 weeks, 82% (914/1,116) of participants sought care. Binary and multinomial logistic regression analyses, adjusted for socio-demographic variables, showed that the elderly were more likely to choose PHC services (OR 1.56; 95% CI: 1.04; 2.35). Moreover, individuals who suffered from hypertension used PHC services more frequently than hospitals (OR 1.94; 95% CI: 1.32; 2.85). A positive association was found between living in an urban area and seeking care for NCDs at polyclinics (OR 10.1; 95% CI: 2.1; 50.1). There was no significant gender difference observed with regard to the type of provider consulted.

Conclusion: Public facilities were reported as the main providers for initiating care and the main providers used in the 8 weeks prior to the interview. While a majority of elderly people visited a PHC to initiate treatment (and follow up) on their chronic conditions, a substantial proportion of adults (aged 18–59) initiated and sought regular NCD care at a hospital. Educating patients and caregivers on active participation in NCD prevention, management, and control through the PHC level should be a long-term effort, along with the establishment of well-structured referral mechanisms and integrated care systems.
The Study of Factors on the Small and Medium Enterprises' Adoption of Mobile Payment: Implications for the COVID-19 Era
Authors: Cao Tianming
Published: March 2021

The coronavirus disease 2019 (COVID-19) pandemic pushes people looking for shopping alternatives, seeking to avoid handling cash in favor of a safe and quick mobile payment. At this juncture, this paper examines the determinants of the adoption of mobile payment services among small and medium enterprises (SMEs) in China. The study proposes four-dimensional factors (business factors, technological competence, environment, and consumers' intentions) based on the literature review findings to understand the challenges of adopting mobile payment. A questionnaire is designed to solicit information from the participants. The findings reveal that business factors, technological competencies of SMEs in China, and the environment positively influence mobile payment adoption. Consumer intention has almost no influence on the adoption of mobile payment. Potential implications for the COVID-19 era are also discussed.
When Maintaining Relationships and Social Connectivity Matter: The Case of New Zealand Midwives and COVID-19
Authors: Crowther Susan , Maude Robyn , Bradford Billie , Austin Diana , Gilkison Andrea , McAra-Couper Judith , Krisjanous Jayne
Published: March 2021

New Zealand’s response to COVID-19 was go hard and go early into Level 4 lockdown on 25th March 2020. This rapid response has resulted in low rates of infection and deaths. For New Zealand midwives, the sudden changes to how they work with women and families during pregnancy, birth and postnatally, especially in the community, required unprecedented innovation and adaptation. The volume of information coming from many different sources, and the speed with which it was changing and updating, added further stress to the delivery of a midwifery model of care underpinned by partnership, collaboration, informed choice, safety and relational continuity. Despite the uncertainties, midwives continued their care for women and their families across all settings. In the rapidly changing landscape of the pandemic, news media provided a real time account of midwives’ and families’ challenges and experiences. This article provides background and discussion of these events and reports on a content analysis of media reporting the impact on the maternity system in New Zealand during the initial surge of the COVID-19 pandemic. We found that the New Zealand midwife was a major influencer and initiator for relational care to occur uninterrupted at the frontline throughout the COVID-19 lockdown, despite the personal risk. The initial 5-week lockdown in March 2020 involved stringent restrictions requiring all New Zealanders, other than essential workers such as midwives, to remain at home. Midwives kept women, their families and communities central to the conversation throughout lockdown whilst juggling their concerns about keeping themselves and their own families safe. Insights gained from the media analysis suggest that despite the significant stress and upheaval experienced by midwives and wāhine/women, relational continuity facilitates quality and consistent care that honors women’s choices and cultural needs even during situations of national crisis.
“Take Me Back to My Homeland Dead or Alive!”: The Myth of Return Among London’s Turkish-Speaking Community
Authors: Cakmak Mustafa
Published: March 2021

In classical diaspora literature, the “myth of return” has major significance. It is believed that the “myth of return” is embedded in the minds of immigrants from their arrival. This paper examines post-migration mobilities of the Turkish-speaking community in North London as well as the shift in narratives of homeland among diaspora communities; from the “myth of return” to ritual-like visits or mundane pilgrimages. My ethnographic study analyses the post-migration homeland visiting patterns of the Turkish-speaking community into four categories. I discuss how narratives of episodic homeland visits and the desire to be buried in the homeland have replaced the myth of return.
Prevalence and Antimicrobial Resistance of Salmonella Isolated From Dead-in-Shell Chicken Embryos in Shandong, China
Authors: Zhao Xiaonan , Ju Zijing , Wang Guisheng , Yang Jie , Wang Fangkun , Tang Hui , Zhao Xiaomin , Sun Shuhong
Published: March 2021

The present study was designed to explore the Salmonella prevalence and antimicrobial resistance characteristics in the context of chick mortality at hatching in China. Between December 2015 and August 2017, 1,288 dead-in-shell chicken embryos were collected from four breeder chicken hatcheries in Tai'an, Rizhao, Jining, and Heze, China. Salmonella isolates were successfully recovered from 6.7% of these embryos (86/1,288) and were evaluated for serotype, antimicrobial susceptibility, Class 1 integron prevalence, antimicrobial resistance gene expression, and multilocus sequence typing (MLST). Salmonella Thompson (37.2%), and Salmonella Infantis (32.6%) were the two most prevalent isolates in these chicken embryos, and 66.3% of isolates exhibited robust ampicillin resistance, while 55.8% of isolates exhibited multi-drug resistance (MDR). The majority of isolates harbored the blaTEM gene (74.4%), with the qnrS gene also being highly prevalent (50.0%). In contrast, just 27.9% of these isolates carried Class 1 integrons. These 86 isolates were separated into four sequence types (STs), whereby ST26 (32.2%) was the most prevalent. Overall, these results suggested that Salmonella infections may be an important cause of chicken embryo mortality in China, and that efforts to support the appropriate use of antibiotics in managing poultry populations are essential.
Space-Efficient, Fast and Exact Routing in Time-Dependent Road Networks
Authors: Strasser Ben , Wagner Dorothea , Zeitz Tim
Published: March 2021

We study the problem of quickly computing point-to-point shortest paths in massive road networks with traffic predictions. Incorporating traffic predictions into routing allows, for example, to avoid commuter traffic congestions. Existing techniques follow a two-phase approach: In a preprocessing step, an index is built. The index depends on the road network and the traffic patterns but not on the path start and end. The latter are the input of the query phase, in which shortest paths are computed. All existing techniques have large index size, slow query running times or may compute suboptimal paths. In this work, we introduce CATCHUp (Customizable Approximated Time-dependent Contraction Hierarchies through Unpacking), the first algorithm that simultaneously achieves all three objectives. The core idea of CATCHUp is to store paths instead of travel times at shortcuts. Shortcut travel times are derived lazily from the stored paths. We perform an experimental study on a set of real world instances and compare our approach with state-of-the-art techniques. Our approach achieves the fastest preprocessing, competitive query running times and up to 38 times smaller indexes than competing approaches.
Family Firms and Coupling among CSR Disclosures and Performance
Authors: Parra-Domínguez Javier , David Fátima , Azevedo Tania
Published: March 2021

This paper aims to analyse the behaviours related to the decoupling of the disclosed information on Corporate Social Responsibility (CSR) and corporate sustainability, deepening these practices’ knowledge within family businesses. For this purpose, we defined decoupling as a gap between social responsibility performance (internal actions) and disclosures (external actions). For a sample of 33,809 observations for the period 2011–2019, corresponding to 5029 companies, 19% being family firms, our empirical evidence supports that family firms present a less wide gap between performance and disclosure, confirming the prevalence of socioemotional wealth dimensions in the decision-making of these companies. In firms without controlled shareholders, the quality of nonfinancial reporting could be understood as ambiguous, understanding that the most useful CSR information is found in the reports of family-owned companies.
Unmanned Aerial Vehicle Operating Mode Classification Using Deep Residual Learning Feature Extraction
Authors: Swinney Carolyn J. , Woods John C.
Published: March 2021

Unmanned Aerial Vehicles (UAVs) undoubtedly pose many security challenges. We need only look to the December 2018 Gatwick Airport incident for an example of the disruption UAVs can cause. In total, 1000 flights were grounded for 36 h over the Christmas period which was estimated to cost over 50 million pounds. In this paper, we introduce a novel approach which considers UAV detection as an imagery classification problem. We consider signal representations Power Spectral Density (PSD); Spectrogram, Histogram and raw IQ constellation as graphical images presented to a deep Convolution Neural Network (CNN) ResNet50 for feature extraction. Pre-trained on ImageNet, transfer learning is utilised to mitigate the requirement for a large signal dataset. We evaluate performance through machine learning classifier Logistic Regression. Three popular UAVs are classified in different modes; switched on; hovering; flying; flying with video; and no UAV present, creating a total of 10 classes. Our results, validated with 5-fold cross validation and an independent dataset, show PSD representation to produce over 91% accuracy for 10 classifications. Our paper treats UAV detection as an imagery classification problem by presenting signal representations as images to a ResNet50, utilising the benefits of transfer learning and outperforming previous work in the field.
Methods to Prevent Future Severe Animal Welfare Problems Caused by COVID-19 in the Pork Industry
Authors: Grandin Temple
Published: March 2021

In the U.S., the most severe animal welfare problems caused by COViD-19 were in the pork industry. Thousands of pigs had to be destroyed on the farm due to reduced slaughter capacity caused by ill workers. In the future, both short-term and long-term remedies will be needed. In the short-term, a portable electrocution unit that uses scientifically validated electrical parameters for inducing instantaneous unconsciousness, would be preferable to some of the poor killing methods. A second alternative would be converting the slaughter houses to carcass production. This would require fewer people to process the same number of pigs. The pandemic revealed the fragility of large centralized supply chains. A more distributed supply chain with smaller abattoirs would be more robust and less prone to disruption, but the cost of pork would be greater. Small abattoirs can coexist with large slaughter facilities if they process pigs for specialized premium markets such as high welfare pork. The pandemic also had a detrimental effect on animal welfare inspection and third party auditing programs run by large meat buyers. Most in-person audits in the slaughter plants were cancelled and audits were done by video. Video audits should never completely replace in-person audits.
Key Genes Regulating Skeletal Muscle Development and Growth in Farm Animals
Authors: Mohammadabadi Mohammadreza , Bordbar Farhad , Jensen Just , Du Min , Guo Wei
Published: March 2021

Farm-animal species play crucial roles in satisfying demands for meat on a global scale, and they are genetically being developed to enhance the efficiency of meat production. In particular, one of the important breeders’ aims is to increase skeletal muscle growth in farm animals. The enhancement of muscle development and growth is crucial to meet consumers’ demands regarding meat quality. Fetal skeletal muscle development involves myogenesis (with myoblast proliferation, differentiation, and fusion), fibrogenesis, and adipogenesis. Typically, myogenesis is regulated by a convoluted network of intrinsic and extrinsic factors monitored by myogenic regulatory factor genes in two or three phases, as well as genes that code for kinases. Marker-assisted selection relies on candidate genes related positively or negatively to muscle development and can be a strong supplement to classical selection strategies in farm animals. This comprehensive review covers important (candidate) genes that regulate muscle development and growth in farm animals (cattle, sheep, chicken, and pig). The identification of these genes is an important step toward the goal of increasing meat yields and improves meat quality.
Finding Purpose in the Conservation of Biodiversity by the Commingling of Science and Ethics
Authors: Vucetich John A. , Macdonald Ewan A. , Burnham Dawn , Bruskotter Jeremy T. , Johnson Dominic D. P. , Macdonald David W.
Published: March 2021

Averting the biodiversity crisis requires closing a gap between how humans tend to behave, individually and collectively, and how we ought to behave—“ought to” in the sense of behaviors required to avert the biodiversity crisis. Closing that gap requires synthesizing insight from ethics with insights from social and behavioral sciences. This article contributes to that synthesis, which presents in several provocative hypotheses: (i) Lessening the biodiversity crisis requires promoting pro-conservation behavior among humans. Doing so requires better scientific understanding of how one’s sense of purpose in life affects conservation-relevant behaviors. Psychology and virtue-focused ethics indicate that behavior is importantly influenced by one’s purpose. However, conservation psychology has neglected inquiries on (a) the influence of one’s purpose (both the content and strength of one’s purpose) on conservation-related behaviors and (b) how to foster pro-conservation purposes; (ii) lessening the biodiversity crisis requires governance—the regulation of behavior by governments, markets or other organization through various means, including laws, norms, and power—to explicitly take conservation as one of its fundamental purposes and to do so across scales of human behaviors, from local communities to nations and corporations; (iii) lessening the biodiversity crisis requires intervention via governance to nudge human behavior in line with the purpose of conservation without undue infringement on other basic values. Aligning human behavior with conservation is inhibited by the underlying purpose of conservation being underspecified. Adequate specification of conservation’s purpose will require additional interdisciplinary research involving insights from ethics, social and behavioral sciences, and conservation biology.
Diet Transition from High-Forage to High-Concentrate Alters Rumen Bacterial Community Composition, Epithelial Transcriptomes and Ruminal Fermentation Parameters in Dairy Cows
Authors: Ramos Sonny C. , Jeong Chang Dae , Mamuad Lovelia L. , Kim Seon Ho , Kang Seung Ha , Kim Eun Tae , Cho Yong Il , Lee Sung Sill , Lee Sang Suk
Published: March 2021

Effects of changing diet on rumen fermentation parameters, bacterial community composition, and transcriptome profiles were determined in three rumen-cannulated Holstein Friesian cows using a 3 × 4 cross-over design. Treatments include HF-1 (first high-forage diet), HC-1 (first high-concentrate diet), HC-2 (succeeding high-concentrate diet), and HF-2 (second high-forage diet as a recovery period). Animal diets contained Klein grass and concentrate at ratios of 8:2, 2:8, 2:8, and 8:2 (two weeks each), respectively. Ammonia-nitrogen and individual and total volatile fatty acid concentrations were increased significantly during HC-1 and HC-2. Rumen species richness significantly increased for HF-1 and HF-2. Bacteroidetes were dominant for all treatments, while phylum Firmicutes significantly increased during the HC period. Prevotella, Erysipelothrix, and Galbibacter significantly differed between HF and HC diet periods. Ruminococcus abundance was lower during HF feeding and tended to increase during successive HC feeding periods. Prevotellaruminicola was the predominant species for all diets. The RNA sequence analysis revealed the keratin gene as differentially expressed during the HF diet, while carbonic-anhydrase I and S100 calcium-binding protein were expressed in the HC diet. Most of these genes were highly expressed for HC-1 and HC-2. These results suggested that ruminal bacterial community composition, transcriptome profile, and rumen fermentation characteristics were altered by the diet transitions in dairy cows.
Tick Infestation and Piroplasm Infection in Barbarine and Queue Fine de l’Ouest Autochthonous Sheep Breeds in Tunisia, North Africa
Authors: Khamassi Khbou Médiha , Rouatbi Mariem , Romdhane Rihab , Sassi Limam , Jdidi Mohamed , Haile Aynalem , Rekik Mourad , Gharbi Mohamed
Published: March 2021

As ticks and tick-borne pathogens affect the productivity of livestock, searching for genetically resistant breeds to infestation by ticks may represent an alternative to the overuse of chemical drugs. The aim of this study was to assess if there is a difference in tick infestation among the main sheep breeds in Tunisia. The study was carried out between April 2018 and January 2020 in 17 small to middle-sized sheep flocks from 3 regions across Tunisia. Four hundred and thirty-nine ear-tagged ewes from Barbarine (n = 288, 65.6%) and Queue Fine de l’Ouest (QFO) (n = 151, 34.4%) breeds were examined and sampled each trimester. Ticks were identified to the species level, and piroplasms were detected using PCR that targets a common sequence ARNr18S to both Babesia and Theileria genera using catch-all primers. Totally, 707 adult ticks were collected from animals; 91.4% (646/707) of them were Rhipicephalus sanguineus s.l. Queue Fine de l’Ouest animals were markedly less infested by ticks, and no one of them was infected by piroplasms compared to the Barbarine breed. Indeed, during the first four seasons, 21 animals, all from the Barbarine breed, were detected positive for piroplasms. This is the first study in Tunisia about the low susceptibility of QFO ewes to infestation by ticks and to infection by piroplasms. The QFO sheep breed could be raised preferably at high-risk areas of tick occurrence and could be considered in concrete control strategies, including a breeding program.
Microbial Diversity and Community Variation in the Intestines of Layer Chickens
Authors: Xiao Sha-Sha , Mi Jian-Dui , Mei Liang , Liang Juanboo , Feng Kun-Xian , Wu Yin-Bao , Liao Xin-Di , Wang Yan
Published: March 2021

The intestinal microbiota is increasingly recognized as an important component of host health, metabolism and immunity. Early gut colonizers are pivotal in the establishment of microbial community structures affecting the health and growth performance of chickens. White Lohmann layer is a common commercial breed. Therefore, this breed was selected to study the pattern of changes of microbiota with age. In this study, the duodenum, caecum and colorectum contents of white Lohmann layer chickens from same environment control farm were collected and analyzed using 16S rRNA sequencing to explore the spatial and temporal variations in intestinal microbiota. The results showed that the diversity of the microbial community structure in the duodenum, caecum and colorectum increased with age and tended to be stable when the layer chickens reached 50 days of age and the distinct succession patterns of the intestinal microbiota between the duodenum and large intestine (caecum and colorectum). On day 0, the diversity of microbes in the duodenum was higher than that in the caecum and colorectum, but the compositions of intestinal microbes were relatively similar, with facultative anaerobic Proteobacteria as the main microbes. However, the relative abundance of facultative anaerobic bacteria (Escherichia) gradually decreased and was replaced by anaerobic bacteria (Bacteroides and Ruminococcaceae). By day 50, the structure of intestinal microbes had gradually become stable, and Lactobacillus was the dominant bacteria in the duodenum (41.1%). The compositions of dominant microbes in the caecum and colorectum were more complex, but there were certain similarities. Bacteroides, Odoribacter and Clostridiales vadin BB60 group were dominant. The results of this study provide evidence that time and spatial factors are important factors affecting the intestinal microbiota composition. This study provides new knowledge of the intestinal microbiota colonization pattern of layer chickens in early life to improve the intestinal health of layer chickens.
Rolipram Prevents the Formation of Abdominal Aortic Aneurysm (AAA) in Mice: PDE4B as a Target in AAA
Authors: Varona Saray , Puertas Lídia , Galán María , Orriols Mar , Cañes Laia , Aguiló Silvia , Camacho Mercedes , Sirvent Marc , Andrés Vicente , Martínez-González José , Rodríguez Cristina
Published: March 2021

Abdominal aortic aneurysm (AAA) is a common life-threatening condition characterized by exacerbated inflammation and the generation of reactive oxygen species. Pharmacological treatments to slow AAA progression or to prevent its rupture remain a challenge. Targeting phosphodiesterase 4 (PDE4) has been verified as an effective therapeutic strategy for an array of inflammatory conditions; however, no studies have assessed yet PDE4 in AAA. Here, we used angiotensin II (AngII)-infused apolipoprotein E deficient mice to study the involvement of the PDE4 subfamily in aneurysmal disease. PDE4B but not PDE4D was upregulated in inflammatory cells from both experimental and human AAA. The administration of the PDE4 selective inhibitor rolipram (3 mg/kg/day) to AngII-challenged mice (1000 ng/kg bodyweight/min) protected against AAA formation, limiting the progressive increase in the aortic diameter without affecting the blood pressure. The drug strongly attenuated the rise in vascular oxidative stress (superoxide anion) induced by AngII, and decreased the expression of inflammatory markers, as well as the recruitment of macrophages (MAC3+), lymphocytes (CD3+), and neutrophils (ELANE+) into the vessel wall. Rolipram also normalized the vascular MMP2 expression and MMP activity, preserving the elastin integrity and improving the vascular remodelling. These results point to PDE4B as a new therapeutic target for AAA.
Statistical Tolerance Analysis—A Survey on Awareness, Use and Need in German Industry
Authors: Walter Michael S. J. , Klein Christina , Heling Björn , Wartzack Sandro
Published: March 2021

The importance of geometric deviations of components for the aesthetic and functional quality of products has been undisputed for decades. So, it is not surprising that not only have numerous researchers devoted themselves to this field, but also commercial software tools for the analysis and optimization of tolerance specifications (currently already fully integrated in 3D-CAD systems) have been available for around 30 years. However, it is even more surprising that the well-founded specification of tolerances and their analysis using a so-called statistical tolerance analysis are only established in a few companies. There is thus a contradiction between the proclaimed relevance of tolerances and their actual consideration in everyday business life. Thus, the question of the significance of geometric deviations and tolerances as well as the use of statistical tolerance analysis arises. Therefore, a survey among 102 German companies was carried out. The results are presented and discussed in this paper.
Multimodal Unsupervised Speech Translation for Recognizing and Evaluating Second Language Speech
Authors: Lee Yun Kyung , Park Jeon Gue
Published: March 2021

This paper addresses an automatic proficiency evaluation and speech recognition for second language (L2) speech. The proposed method recognizes the speech uttered by the L2 speaker, measures a variety of fluency scores, and evaluates the proficiency of the speaker’s spoken English. Stress and rhythm scores are one of the important factors used to evaluate fluency in spoken English and are computed by comparing the stress patterns and the rhythm distributions to those of native speakers. In order to compute the stress and rhythm scores even when the phonemic sequence of the L2 speaker’s English sentence is different from the native speaker’s one, we align the phonemic sequences based on a dynamic time-warping approach. We also improve the performance of the speech recognition system for non-native speakers and compute fluency features more accurately by augmenting the non-native training dataset and training an acoustic model with the augmented dataset. In this work, we augment the non-native speech by converting some speech signal characteristics (style) while preserving its linguistic information. The proposed variational autoencoder (VAE)-based speech conversion network trains the conversion model by decomposing the spectral features of the speech into a speaker-invariant content factor and a speaker-specific style factor to estimate diverse and robust speech styles. Experimental results show that the proposed method effectively measures the fluency scores and generates diverse output signals. Also, in the proficiency evaluation and speech recognition tests, the proposed method improves the proficiency score performance and speech recognition accuracy for all proficiency areas compared to a method employing conventional acoustic models.
Effect of Freeze–Thaw Cycles on Carbonation Behavior of Three Generations of Repeatedly Recycled Aggregate Concrete
Authors: Liu Hui , Hua Minqi , Zhu Pinghua , Chen Chunhong , Wang Xinjie , Qian Zhongqiu , Dong Yanlong
Published: March 2021

Multiple recycling of waste concrete has attracted widespread attention. This study presented the carbonation behavior of repeatedly recycled aggregate concrete (RRAC) used in a micro-frozen region. The effects of freeze–thaw cycles on the carbonation depth of three generations of RRAC with 25%, 75%, and 100% of replacement rate were evaluated. All RRAC specimens after different numbers of freeze–thaw cycles were rapidly carbonated for 28 d indoors to test the carbonation resistance of concrete. The results suggested that the carbonation depth of RRAC subjected to freeze–thaw cycles is higher than that in the non-freeze–thaw condition. This is because the freeze–thaw damages cause the internal structure of RRAC to become porous and and prone to cracking, thus providing convenient channels for CO2 to react with the alkali in the cementitious materials. With the growth of replacement rate or recycling number, RRAC reveals serious freeze–thaw damage and inferior carbonation resistance, which is due to the continuous deterioration repeatedly recycled concrete aggregate (RRCA) quality. However, when the replacement rate was 25%, the carbonation depth for the third generation of RAC was comparable to the second generation of RAC at a 75% replacement rate, and even the first generation of 100% RAC. To ensure better carbonation resistance durability of multiple recycling RAC, the low replacement rate of RRCA should be considered. For the third generation of RAC with the 100% replacement rate, its highest carbonation depth after freeze–thaw cycles was 9.16 mm, which still met the design requirements for structural use in a micro-frozen region. This indicates that it is feasible for three generations of RRAC to be used in the micro-frozen environment and that RRAC has great engineering application potential and promotional value.
OCT Capillary Depth Measurement in Copper Micro Welding Using Green Lasers
Authors: Beck Tobias , Bantel Christoph , Boley Meiko , Bergmann Jean Pierre
Published: March 2021

The transition of the powertrain from combustion to electric systems increases the demand for reliable copper connections. For such applications, laser welding has become a key technology. Due to the complexity of laser welding, especially at micro welding with small weld seam dimensions and short process times, reliable in-line process monitoring has proven to be difficult. By using a green laser with a wavelength of λ=515 nm, the welding process of copper benefits from an increased absorption, resulting in a shallow and stable deep penetration welding process. This opens up new possibilities for the process monitoring. In this contribution, the monitoring of the capillary depth in micro copper welding, with welding depth of up to 1 mm, was performed coaxially using an optical coherence tomography (OCT) system. By comparing the measured capillary depth and the actual welding depth, a good correlation between two measured values could be shown independently of the investigated process parameters and stability. Measuring the capillary depth allows a direct determination of the present aspect ratio in the welding process. For deep penetration welding, aspect ratios as low as 0.35 could be shown. By using an additional scanning system to superimpose the welding motion with a spacial oscillating of the OCT beam perpendicular to the welding motion, multiple information about the process could be determined. Using this method, several process information can be measured simultaneously and is shown for the weld seam width exemplarily.
How to Make Augmented Reality a Tool for Railway Maintenance Operations: Operator 4.0 Perspective
Authors: Scheffer Sara , Martinetti Alberto , Damgrave Roy , Thiede Sebastian , van Dongen Leo
Published: March 2021

In the last few decades, several initiatives and approaches are set up to support maintenance procedures for the railway industry in adopting the principles of Industry 4.0. Contextualized maintenance technologies such as Augmented Reality (AR) overlay can integrate virtual information on physical objects to improve decision-making and action-taking processes. Operators work in a dynamic working environment requiring both high adaptive capabilities and expert knowledge. There is a need to support the operators with tailor-based information that is customized and contextualized to their expertise and experience. It calls for AR tools and approaches that combine complex methodologies with high usability requirements. The development of these AR tools could benefit from a structured approach. Therefore, the objective of this paper is to propose an adaptive architectural framework aimed at shaping and structuring the process that provides operators with tailored support when using an AR tool. Case study research is applied within a revelatory railway industry setting. It was found that the framework ensures that self-explanatory AR systems can capture the knowledge of the operator, support the operator during maintenance activities, conduct failure analysis, provide problem-solving strategies, and improve learning capabilities. This study contributes to the necessity of having a human-centered approach for the successful adaption of AR technology tools for the railway industry.
Wintertime Cold Extremes in Northeast China and Their Linkage with Sea Ice in Barents-Kara Seas
Authors: Luo Yongyue , Li Chun , Shi Jian , An Xiadong , Sun Yaqing
Published: March 2021

The impacts of Arctic sea ice on the interannual variability of winter extreme low temperature (WELT) in Northeast China (NEC) and the associated atmospheric circulation patterns are explored in this study based on meteorological observation and the National Centers for Environmental Prediction-National Center for Atmospheric Research (NCEP/NCAR) reanalysis data. Results show that WELT in NEC has prominent interannual variability. We further use ±0.8 standard deviation as the threshold to select the years of frequent and rare extreme low temperature anomalies. Using composite analysis, we find that there are significant negative geopotential height anomalies at 500 hPa over NEC and positive geopotential height anomalies along the Arctic region, which represent the intensification of the East Asian trough (EAT) and the negative Arctic Oscillation (AO) phase in the years of more frequent WELT. The opposite characteristics are detected in the years of rare WELT. Furthermore, we determine that the Barents-Kara Seas are key sea ice regions in Arctic area. In the years of frequent WELT, the decrease of autumn Barents-Kara Seas sea ice and the positive sea surface temperature anomaly can last until the following winter, which is conducive to the intensification of anticyclonic anomalies in Ural regions and the northward extension of Ural ridge (UR). The northerly flow in front of UR guides the cold air penetrating southward from polar regions. Moreover, the anomalous cyclone over East Asia deepens the EAT. The northerly wind behind EAT guides the cold air to the NEC region, causing the wintertime low temperature there. The almost opposite situation occurs in the years of rare WELT.