bluuebunny/crossref_metadata_2025 huggingface dataset:
Evaluation of Disability Progression in Multiple Sclerosis via Magnetic-Resonance-Based Deep Learning Techniques
Authors: Taloni Alessandro , Farrelly Francis Allen , Pontillo Giuseppe , Petsas Nikolaos , Giannì Costanza , Ruggieri Serena , Petracca Maria , Brunetti Arturo , Pozzilli Carlo , Pantano Patrizia , Tommasin Silvia
Published: September 2022

Short-term disability progression was predicted from a baseline evaluation in patients with multiple sclerosis (MS) using their three-dimensional T1-weighted (3DT1) magnetic resonance images (MRI). One-hundred-and-eighty-one subjects diagnosed with MS underwent 3T-MRI and were followed up for two to six years at two sites, with disability progression defined according to the expanded-disability-status-scale (EDSS) increment at the follow-up. The patients’ 3DT1 images were bias-corrected, brain-extracted, registered onto MNI space, and divided into slices along coronal, sagittal, and axial projections. Deep learning image classification models were applied on slices and devised as ResNet50 fine-tuned adaptations at first on a large independent dataset and secondly on the study sample. The final classifiers’ performance was evaluated via the area under the curve (AUC) of the false versus true positive diagram. Each model was also tested against its null model, obtained by reshuffling patients’ labels in the training set. Informative areas were found by intersecting slices corresponding to models fulfilling the disability progression prediction criteria. At follow-up, 34% of patients had disability progression. Five coronal and five sagittal slices had one classifier surviving the AUC evaluation and null test and predicted disability progression (AUC > 0.72 and AUC > 0.81, respectively). Likewise, fifteen combinations of classifiers and axial slices predicted disability progression in patients (AUC > 0.69). Informative areas were the frontal areas, mainly within the grey matter. Briefly, 3DT1 images may give hints on disability progression in MS patients, exploiting the information hidden in the MRI of specific areas of the brain.
The Sympathetic Nervous System Contributes to the Establishment of Pre-Metastatic Pulmonary Microenvironments
Authors: Ieguchi Katsuaki , Funakoshi Masabumi , Mishima Taishi , Takizawa Kohtaro , Omori Tsutomu , Nakamura Fumio , Watanabe Makoto , Tsuji Mayumi , Kiuchi Yuji , Kobayashi Shinichi , Tsunoda Takuya , Maru Yoshiro , Wada Satoshi
Published: September 2022

Emerging evidence suggests that neural activity contributes to tumor initiation and its acquisition of metastatic properties. More specifically, it has been reported that the sympathetic nervous system regulates tumor angiogenesis, tumor growth, and metastasis. The function of the sympathetic nervous system in primary tumors has been gradually elucidated. However, its functions in pre-metastatic environments and/or the preparation of metastatic environments far from the primary sites are still unknown. To investigate the role of the sympathetic nervous system in pre-metastatic environments, we performed chemical sympathectomy using 6-OHDA in mice and observed a decrease in lung metastasis by attenuating the recruitment of myeloid-derived suppressor cells. Furthermore, we note that neuro-immune cell interactions could be observed in tumor-bearing mouse lungs in conjunction with the decreased expression of Sema3A. These data indicate that the sympathetic nervous system contributes to the preparation of pre-metastatic microenvironments in the lungs, which are mediated by neuro-immune cell interactions.
A Comprehensive Characterization of Stemness in Cell Lines and Primary Cells of Pancreatic Ductal Adenocarcinoma
Authors: Ferrara Benedetta , Dugnani Erica , Sordi Valeria , Pasquale Valentina , Pellegrini Silvia , Reni Michele , Balzano Gianpaolo , Piemonti Lorenzo
Published: September 2022

The aim of this study is to provide a comprehensive characterization of stemness in pancreatic ductal adenocarcinoma (PDAC) cell lines. Seventeen cell lines were evaluated for the expression of cancer stem cell (CSC) markers. The two putative pancreatic CSC phenotypes were expressed heterogeneously ranging from 0 to 99.35% (median 3.46) for ESA+CD24+CD44+ and 0 to 1.94% (median 0.13) for CXCR4+CD133+. Cell lines were classified according to ESA+CD24+CD44+ expression as: Low-Stemness (LS; <5%, n = 9, median 0.31%); Medium-Stemness (MS; 6–20%, n = 4, median 12.4%); and High-Stemness (HS; >20%, n = 4, median 95.8%) cell lines. Higher degree of stemness was associated with in vivo tumorigenicity but not with in vitro growth kinetics, clonogenicity, and chemo-resistance. A wide characterization (chemokine receptors, factors involved in pancreatic organogenesis, markers of epithelial–mesenchymal transition, and secretome) revealed that the degree of stemness was associated with KRT19 and NKX2.2 mRNA expression, with CD49a and CA19.9/Tie2 protein expression, and with the secretion of VEGF, IL-7, IL-12p70, IL-6, CCL3, IL-10, and CXCL9. The expression of stem cell markers was also evaluated on primary tumor cells from 55 PDAC patients who underwent pancreatectomy with radical intent, revealing that CXCR4+/CD133+ and CD24+ cells, but not ESA+CD24+CD44+, are independent predictors of mortality.
The Role of T Cells in Systemic Sclerosis: An Update
Authors: Sakkas Lazaros I. , Bogdanos Dimitrios P.
Published: September 2022

Systemic sclerosis (SSc) is a chronic disease characterized by microvasculopathy, autoantibodies (autoAbs), and fibrosis. The pathogenesis of the disease is incompletely understood. Microvasculopathy and autoAbs appear very early in the disease process. AutoAbs, such as those directed against DNA topoisomerase I (Topo I), are disease specific and associated with disease manifestations, and indicate activation of the adaptive immune system. B cells are involved in fibrosis in SSc. T cells are also involved in disease pathogenesis. T cells show signs of antigen-induced activation; T cells of TH2 type are increased and produce profibrotic cytokines interleukin (IL)-4, IL-13, and IL-31; CD4+ cytotoxic T lymphocytes are increased in skin lesions, and cause fibrosis and endothelial cell apoptosis; circulating T follicular helper (TFH) cells are increased in SSc produce IL-21 and promote plasmablast antibody production. On the other hand, regulatory T cells are impaired in SSc. These findings provide strong circumstantial evidence for T cell implication in SSc pathogenesis and encourage new T cell-directed therapeutic strategies for the disease.
Effect of Phosphorus Content on Magnetic and Mechanical Properties of Non-Oriented Electrical Steel
Authors: He Qinyu , Liu Yulong , Zhu Chengyi , Xie Xiaohui , Zhu Rong , Li Guangqiang
Published: September 2022

The effect of target phosphorus (P) content on the precipitates, microstructure, texture, magnetic properties, and mechanical properties of low-carbon (C) and low-silicon (Si) non-oriented electrical steel (NOES) was investigated and the influence mechanism was clarified. The results indicate that the precipitates in the steels are mainly aluminum (Al)-manganese (Mn)-Si-bearing complex nitrides ((Al,Si,Mn)xNy) and P-bearing complex nitrides ((Al,Si,Mn)xNy-P). Increasing target phosphorus content in the steels decreases (Al,Si,Mn)xNy, and increases (Al,Si,Mn)xNy-P. The number density of the precipitates is the lowest, and the average size of the precipitates and grain size of the finished steel is the largest in the samples with target P content at the 0.14% level (0.14%P-targeted). The average grain size and microstructure homogeneity of the steels are influenced by the addition of phosphorus. The content of the {111}<112> component decreases, and the favorable texture increases after phosphorus is added to the steel. The magnetic induction of the steel is improved. Grain refinement and microstructure inhomogeneity lead to an iron loss increase after target phosphorus content increases in the steel. The best magnetic induction B50 is 1.765 T in the 0.14%P-targeted samples. The tensile strength and yield strength are improved owing to solid solution strengthening and the grain refinement effect of phosphorus added to the steels.
Metasurfaces for Amplitude-Tunable Superposition of Plasmonic Orbital Angular Momentum States
Authors: Zhang Yuqin , An Jianshan , An Xingqi , Zeng Xiangyu , He Changwei , Liu Guiyuan , Cheng Chuanfu , Song Hongsheng
Published: September 2022

The superposition of orbital angular momentum (OAM) in a surface plasmon polariton (SPP) field has attracted much attention in recent years for its potential applications in classical physics problems and quantum communications. The flexible adjustment of the amplitudes of two OAM states can provide more freedom for the manipulation of superposed states. Here, we propose a type of plasmonic metasurface consisting of segmented spiral-shaped nanoslits that not only can generate the superposition of two OAM states with arbitrary topological charges (TCs), but also can independently modulate their relative amplitudes in a flexible manner. The TCs of two OAM states can be simultaneously modulated by incident light, the rotation rate of the nanoslits, and the geometric parameters of the segmented spiral. The relative amplitudes of the two OAM states are freely controllable by meticulously tuning the width of the nanoslits. Under a circularly polarized beam illumination, two OAM states of opposite TCs can be superposed with various weightings. Furthermore, hybrid superposition with different TCs is also demonstrated. The presented design scheme offers an opportunity to develop practical plasmonic devices and on-chip applications.
Corrosion Resistance of Laser Powder Bed Fused AISI 316L Stainless Steel and Effect of Direct Annealing
Authors: Bae Kichang , Shin Dongmin , Lee Jonghun , Kim Seohan , Lee Wookjin , Jo Ilguk , Lee Junghoon
Published: September 2022

Alloy parts produced by an additive manufacturing method with rapid heat transfer from fast melting and solidification have different microstructures, characteristics, and performances compared with materials made by the conventional process. In this study, the corrosion and oxidation resistance of SS316L, which was prepared by the powder bed fusion process, was compared with those of cold-rolled SS316L. Additionally, the surface oxide film on stainless steel was thoroughly assessed since the film has the greatest influence on the corrosion and oxidation resistance. The effect of heat treatment on corrosion and oxidation resistance of SS316L fabricated by additive manufacturing was investigated. The SS316L has a microstructure formed by sub-grain cells, in which locally concentrated alloying elements form a stable passive film. As a result, it has a higher level of corrosion resistance and oxidation resistance than conventional cold-rolled materials. However, it was confirmed that the sub-grain cell was removed by heat treatment, which resulted in the degradation of corrosion and oxidation resistance.
Comparison of Mechanical and Crack-Healing Properties of PE-PVA Hybrid Fiber-Reinforced SHCCs in Natural and Underwater Conditions
Authors: Park Se-Eon , Nguyễn Huy Hoàng , Choi Jeong-Il , Lee Bang Yeon , Kim Yun Yong
Published: September 2022

This paper presents a direct comparison of the mechanical and crack-healing properties of strain hardening cementitious composites (SHCC) under water submersion in a laboratory and in a natural environment outdoors. Portland cement, slag, crumb rubber powder, and hybrid polyethylene and polyvinyl alcohol fibers were used for the SHCC, and mixture proportions were determined. Specimens were exposed to different environmental conditions. A sequence of experimental tests including those for density, compressive strength, and tensile properties was performed to assess the mechanical properties of the SHCC. To confirm the healing feasibility of the SHCC, crack width reduction, stiffness recovery, and tensile performance at post-healing were adopted. The test results showed that underwater conditions are better than natural conditions in improving both the mechanical and crack-healing properties of SHCC. Specifically, the SHCC cured in natural conditions had a lower compressive strength, tensile strength, and tensile strain capacity than that cured in underwater conditions by 10%, 4%, and 3%, respectively. The SHCC cured in underwater conditions had a healing threshold of crack width of 60 µm, while the SHCC cured in natural conditions had very limited crack-healing capacity. Additionally, stiffness recovery of the SHCC cured in underwater conditions was higher than that cured in natural conditions.
Influence of Hydrogen Plasma on the Surface Structure of Beryllium
Authors: Skakov Mazhyn , Batyrbekov Erlan , Sokolov Igor , Miniyazov Arman , Tulenbergenov Timur , Sapataev Yerzhan , Orazgaliyev Nurkhat , Bukina Olga , Zhanbolatova Gainiya , Kozhakhmetov Yernat
Published: September 2022

This paper presents the research results of hydrogen plasma effect on the surface structure of the TGP-56 beryllium. In the linear simulator, the operating conditions of the first wall of ITER are simulated. Beryllium was irradiated with hydrogen plasma at surface temperatures of ~360 °C, ~800 °C, and ~1200 °C, depending on its location in the ITER chamber; with a different number of pulses with a duration of each pulse of 500 s. Samples of irradiated beryllium were subjected to a set of material studies. Experimental data were obtained on the change in the structure of the surface and edges of the beryllium samples after the plasma effect. It was found that at normal (2 MW/m2) and increased (4.7 MW/m2) heat fluxes on the first wall of the ITER, the edges and beryllium surface have good resistance to erosion. Under critical conditions close to the melting point, beryllium strongly erodes and evaporates. It has been established that this material has a high resource resistance to hydrogen plasma effect in the ITER under operating conditions.
Surface Evaluation of Aligners after Immersion in Coca-Cola and Orange Juice
Authors: Warnecki Maciej , Sarul Michał , Kozakiewicz Marcin , Zięty Anna , Babiarczuk Bartosz , Kawala Beata , Jurczyszyn Kamil
Published: September 2022

Orthodontic removable appliances made of transparent thermoplastic materials—aligners—are becoming increasingly popular in contemporary orthodontic practice. It is important for the clinician to fully understand the mechanical properties and behavior of the appliance used. Because of that, the aim of our study was to investigate the changes in aligner surface after immersion in Coca-Cola and orange juice. For surface evaluation, fractal analysis, texture analysis, and wetting angle measurement were performed. Statistically significant changes were found between some of the groups in the fractal dimension analysis. In texture analysis, all but one intergroup comparison showed statistically significant differences. For wetting angle assessment, statistically significant differences were found. These were, however, more numerous when assessing glycol droplets, rather than water droplets. Fractal dimension analysis confirmed a correlation between the intensity of changes in the aligner surface with immersion time in the liquids assessed. Texture analysis showed a high sensitivity to the changes in aligner surface. It failed, however, to reveal changes relative to immersion time. Wetting angle analysis revealed aligner surface degradation for Coca-Cola. It did not, however, prove the dependence of the intensity of this degradation as a function of time. Both Coca-Cola and orange juice can cause aligner surface degradation.
Synthesis and Properties of Optically Transparent Fluoro-Containing Polyimide Films with Reduced Linear Coefficients of Thermal Expansion from Organo-Soluble Resins Derived from Aromatic Diamine with Benzanilide Units
Authors: Ren Xi , Wang Hanli , Du Xuanzhe , Qi Haoran , Pan Zhen , Wang Xiaolei , Dai Shengwei , Yang Changxu , Liu Jingang
Published: September 2022

Wholly aromatic polyimide (PI) films with good solution processability, light colors, good optical transparency, high storage modulus, and improved heat resistance were prepared and characterized. For this purpose, a multi-component copolymerization methodology was performed from a fluoro-containing dianhydride, 4,4′-(hexafluoroisopropylidene)diphthalic anhydride (6FDA), a rigid dianhydride, 3,3′,4,4′-biphenyltetracarboxylic dianhydride (BPDA), and a fluoro-containing diamine, 2,2′-bis(trifluoromethyl)-4,4′-bis [4-(4-amino-3-methyl)benzamide]biphenyl (MABTFMB). One homopolymer, FPI-1 (6FDA-MABTFMB), and five copolymers, FPI-2~FPI-6, containing the BPDA units from 10 mol% to 50 mol% in the dianhydride moieties, were prepared, respectively. The derived PI resins showed good solubility in the polar aprotic solvents, such as N-methyl-2-pyrrolidone (NMP) and N,N-dimethylacetamide (DMAc). The flexible PI films obtained by the solution casting procedure showed good optical properties with the transmittances higher than 74.0% at the wavelength of 450 nm. The PI films exhibited excellent thermal properties, including 5% weight loss temperatures (T5%) over 510 °C, together with glass transition temperatures (Tg) over 350.0 °C according to the peak temperatures of the loss modulus in dynamical mechanical analysis (DMA) measurements. The FPI-6 film also showed the lowest linear coefficient of thermal expansion (CTE) value of 23.4 × 10−6/K from 50 to 250 °C according to the thermomechanical analysis (TMA) measurements, which was obviously lower than that of FPI-1 (CTE = 30.6 × 10−6/K).
Micro-Mechanical Properties of Slag Rim Formed in Cement–Slag System Evaluated by Nanoindentation Combined with SEM
Authors: Zhang Yu , Liang Minfei , Gan Yidong , Çopuroğlu Oğuzhan
Published: September 2022

Slag rim mainly consists of secondary precipitations such as C–S–H gel phase and hydrotalcite-like phase, which originate from the hydration of slag. In this paper, the micro-mechanical properties of slag rim were characterized by nanoindentation in combination with SEM. It was found that, compared to the C–S–H gel phase, slag rim showed about a 15 GPa higher modulus of elasticity. At the early age, slag cement paste mainly consisted of low-density C–S–H gel phase, high-density C–S–H gel phase, and unhydrated slag particles, as well as calcium hydroxide based on the results of nanoindentation; at the later age, the system contained C–S–H gel phase, slag rim (a mixture of C–S–H gel phase and hydrotalcite-like phase), and unhydrated slag particles.
A Finite-Difference Based Parallel Solver Algorithm for Online-Monitoring of Resistance Spot Welding
Authors: Teren Tomas , Penter Lars , Peukert Christoph , Ihlenfeldt Steffen
Published: September 2022

Although resistance spot welding (RSW) was invented at the beginning of the last century, the online-monitoring and control of RSW is still a technological challenge and of economic and ecological importance. Process, material and geometry parameters of RSW are stored in the database of the process control system. Prospectively, these accumulated data could serve as the base for data-driven and physics-based models to monitor the spot weld process in real-time. The objective of this paper is to present a finite-difference based parallel solver algorithm to simulate RSW time-efficiently. The Peaceman–Rachford scheme was combined with the Thomas algorithm to compute the electrical–thermal interdependencies of the resistance spot welding process within seconds. Finally, the electric–thermal model is verified by a convergence analysis and parameter study.
The Effect of Tin on Microstructure and Properties of the Al-10 wt.% Si Alloy
Authors: Kozana Janusz , Piękoś Marcin , Garbacz-Klempka Aldona , Perek-Nowak Małgorzata
Published: September 2022

In this paper, the results from studies regarding near-eutectic Al-Si alloys with Sn as an alloying addition are presented. In most Al-Si alloys, tin is regarded as a contaminant; thus, its amount is limited to up to 0.3 wt.%. The few studies that can be found in the literature regarding the behaviour of tin in aluminium alloys suggest the beneficial effect of this element on selected properties. However, these results were obtained for hypereutectic Al-Si alloys or wrought aluminium alloys. In our studies, the influence of tin contents of up to 1.7 wt.% was determined on the AlSi10 alloy. Thermal analysis, measurements of the mechanical properties of the cast and heat-treated alloy, metallographic observations (light microscopy, scanning electron microscopy), and EDS (X-ray energy dispersive spectrometry) measurement allowed us to fully describe the effect of tin on the aluminium alloy. The results of the thermal analysis showed changes in the range of the α-Al solution crystallisation and the α+β eutectic through a decrease in the alloy’s solidification start point and eutectic solidification point. As a result, the elongation of the alloy was more than double in the AlSi10Sn1.7 alloy, with an A5 value of 8.1% and a tensile strength that was above 200 MPa.
Micron-Scale Biogeography of Seawater Biofilm Colonies at Submersed Solid Substrata Affected by Organic Matter and Microbiome Transformation in the Baltic Sea
Authors: Grzegorczyk Maciej , Pogorzelski Stanislaw , Janowicz Paulina , Boniewicz-Szmyt Katarzyna , Rochowski Pawel
Published: September 2022

The aim of this research was to determine temporal and spatial evolution of biofilm architecture formed at model solid substrata submersed in Baltic sea coastal waters in relation to organic matter transformation along a one-year period. Several materials (metals, glass, plastics) were deployed for a certain time, and the collected biofilm-covered samples were studied with a confocal microscopy technique using the advanced programs of image analysis. The geometric and structural biofilm characteristics: biovolume, coverage fraction, mean thickness, spatial heterogeneity, roughness, aggregation coefficient, etc., turned out to evolve in relation to organic matter transformation trends, trophic water status, microbiome evolution, and biofilm micro-colony transition from the heterotrophic community (mostly bacteria) to autotrophic (diatom-dominated) systems. The biofilm morphology parameters allowed the substratum roughness, surface wettability, chromatic organisms colony adaptation to substrata, and quorum sensing or cell to cell signaling effects to be quantitatively evaluated. In addition to the previous work, the structural biofilm parameters could become further novel trophic state indicators.
Comparison of Conventional and Robotic Fused Filament Fabrication on Silicone Build Plates
Authors: Herzog Thomas , Schnell Georg , Tille Carsten , Seitz Hermann
Published: September 2022

The objective of this study is the investigation of the transferability of the material extrusion process from conventional to robotic fabrication on silicone build plates for use in Enhanced Multipoint Moulding with Additive Attachments. Therefore, the study is based on two series of experiments. The first series of tests used a conventional plant extended by a silicone construction platform. In comparison, a six-axis industrial robot was chosen to produce the test specimens in the second series of tests. The comparisons of adhesion strengths and relative shape deviations are used to validate the transferability. The results of the tests show a very good transferability of the process from conventional to robotic production. Whilst angular specimen geometries can be transferred directly, for round specimen geometries, the results show a need for further adaptation to the robot kinematics. The round specimen geometries showed deviations in the surface quality caused by an over-extrusion in the robotic manufacturing. This over-extrusion results from the slicing process in combination with the robot control and may be avoided through further optimisation of the process parameters. Overall, to the best of our knowledge, this study is the first that successfully demonstrates the transfer of Fused Filament Fabrication (FFF) from a conventional system to manufacturing using robots on silicone build plates for the use in Enhanced Multipoint Moulding with Additive Attachments.
Facile Approach to the Fabrication of Highly Selective CuCl-Impregnated θ-Al2O3 Adsorbent for Enhanced CO Performance
Authors: Jeong Cheonwoo , Kim Joonwoo , Baik Joon Hyun , Pandey Sadanand , Koh Dong Jun
Published: September 2022

We have developed a facile and sustainable method to produce a novel θ-Al2O3-supported CuCl adsorbent through impregnation methods using CuCl2 as the precursor. In an easy two-step process, θ-Al2O3 was impregnated with a known concentration of CuCl2 solutions, and the precursor was calcined to prepare CuCl oversupport. The developed novel θ-Al2O3-supported CuCl adsorbent was compared with an adsorbent prepared through the conventional method using CuCl salt. The adsorbents were characterized via X-ray diffraction (XRD), thermal gravimetric analysis (TGA) and temperature-programmed reduction (H2-TPR). Overall, the adsorbent indicates a high CO adsorption capacity, high CO/CO2 and CO/N2 selectivity, and remarkable reusability performance. This process is operated at ambient temperature, which minimizes operation costs in CO separation processes. In addition, these results indicate that the systematic evaluation of alumina-supported CuCl adsorbent can provide significant insight for designing a realistic PSA process for selective CO separation processes.
Effect of Multiple Annular Plates on Vibration Characteristics of Laminated Submarine-like Structures
Authors: Chen Zhengxiong , Zhong Rui , Hu Shuangwei , Qin Bin , Zhao Xing
Published: September 2022

A numerical model for the prediction of vibration behaviors of a laminated submarine structure consisting of spherical, cylindrical, and cone shells with multiple built-in annular plates is reported in this article. With the aid of the first-order shear deformation theory (FSDT) concerning plates and shells, the energy expressions of each substructure are derived. The displacement functions in the energy functionals are expanded by the employment of Legendre orthogonal polynomials and circumferential Fourier series. Then, the Rayleigh–Ritz procedure is performed to obtain the eigenfrequency and the corresponding eigenmode of the submarine model. The correctness of the structural model is examined by comparing the results with existing papers and the finite element method, and the maximum deviation is not more than 2.07%. Additionally, the influence of the plate’s thickness, position, inner diameter, as well as the laying angle on the intrinsic vibration characteristics of laminated submarine-like structure is determined. The results reveal that rational geometry design and assemblage benefit the vibration performance of the combination. Increasing the thickness of all the annular plates, decreasing the inner radius, and regulating the laminated scheme, make remarkable influence on structural free vibration, with the maximum relative changing rate of frequency exceeding 97%, 16%, and 23%, respectively.
Evaluation of Surface Roughness Parameters of HDF for Finishing under Industrial Conditions
Authors: Henke Milena , Lis Barbara , Krystofiak Tomasz
Published: September 2022

One of the most important properties of the surface of wood-based panels is their roughness. This property determines the way of working with the material in the processes of gluing and surface varnishing. The aim of this study was to determine the effect of various sanding belt configurations and the feeding speed of the conveyor belt during grinding on the surface roughness of high-density fiberboards (HDF). The research material was prepared under industrial conditions. Three types of boards were selected for the tests. After grinding, the roughness parameters were measured both transversely and longitudinally relative to the grinding direction, using a Mitutoyo SJ-210 profilometer and the optical method. Based on ANOVA analysis of the data, it was found that the type of HDF boards used and the configuration of the abrasive belts had a statistically significant impact on the roughness. The samples for which the grinding process was performed with sanding belts of the highest grain size had the lowest roughness. For the amplitude roughness parameters, the direction of roughness measurement had a significant influence. These results may provide valuable guidance for the furniture industry in the preparation of HDF for furniture production.
Effect of Preheating on the Residual Stress and Material Properties of Inconel 939 Processed by Laser Powder Bed Fusion
Authors: Malý Martin , Nopová Klára , Klakurková Lenka , Adam Ondřej , Pantělejev Libor , Koutný Daniel
Published: September 2022

One of the main limitations of laser powder bed fusion technology is the residual stress (RS) introduced into the material by the local heating of the laser beam. RS restricts the processability of some materials and causes shape distortions in the process. Powder bed preheating is a commonly used technique for RS mitigation. Therefore, the objective of this study was to investigate the effect of powder bed preheating in the range of room temperature to 400 °C on RS, macrostructure, microstructure, mechanical properties, and properties of the unfused powder of the nickel-based superalloy Inconel 939. The effect of base plate preheating on RS was determined by an indirect method using deformation of the bridge-shaped specimens. Inconel 939 behaved differently than titanium and aluminum alloys when preheated at high temperatures. Preheating at high temperatures resulted in higher RS, higher 0.2% proof stress and ultimate strength, lower elongation at brake, and higher material hardness. The increased RSs and the change in mechanical properties are attributed to changes in the microstructure. Preheating resulted in a larger melt pool, increased the width of columnar grains, and led to evolution of the carbide phase. The most significant microstructure change was in the increase of the size and occurrence of the carbide phase when higher preheating was applied. Furthermore, it was detected that the evolution of the carbide phase strongly corresponds to the build time when high-temperature preheating is applied. Rapid oxidation of the unfused powder was not detected by EDX or XRD analyses.
Assembling of Carbon Fibre/PEEK Composites: Comparison of Ultrasonic, Induction, and Transmission Laser Welding
Authors: Korycki Adrian , Garnier Christian , Bonmatin Margot , Laurent Elisabeth , Chabert France
Published: September 2022

In the present work, an ultrasonic, an induction, and a through transmission laser welding were compared to join carbon fibre reinforced polyetheretherketone (CF/PEEK) composites. The advantages and drawbacks of each process are discussed, as well as the material properties required to fit each process. CF/PEEK plates were consolidated at 395 °C with an unidirectional sequence and cross-stacking ply orientation. In some configurations, a polyetherimide (PEI) layer or substrate was used. The thermal, mechanical, and optical properties of the materials were measured to highlight the specific properties required for each process. The drying conditions were defined as 150 °C during at least 8 h for PEI and 24 h for CF/PEEK to avoid defects due to water. The optical transmission factor of PEI is above 40% which makes it suitable for through transmission laser welding. The thermal conductivity of CF/PEEK is at most 55 W·(m·K)−1, which allows it to weld by induction without a metallic susceptor. Ultrasonic welding is the most versatile process as it does not necessitate any specific properties. Then, the mechanical resistance of the welds was measured by single lap shear. For CF/PEEK on CF/PEEK, the maximum lap shear strength (LSS) of 28.6 MPa was reached for a joint obtained by ultrasonic welding, while an induction one brought 17.6 MPa. The maximum LSS of 15.2 MPa was obtained for PEI on CF/PEEK assemblies by laser welding. Finally, interfacial resistances were correlated to the fracture modes through observations of the fractured surfaces. CF/PEEK on CF/PEEK joints resulted in mixed cohesive/adhesive failure at the interface and within the inner layers of both substrates. This study presents a guideline to select the suitable welding process when assembling composites for the aerospace industry.
Designing a Biomaterial Approach to Control the Adaptive Response to a Skin Injury
Authors: Feldman Dale
Published: September 2022

The goal of this review is to explain how to design a biomaterial approach to control the adaptive response to injury, with an emphasis on skin wounds. The strategies will be selected based on whether they have a reasonable probability of meeting the desired clinical outcome vs. just comparing the pros and cons of different strategies. To do this, the review will look at the normal adaptive response in adults and why it does not meet the desired clinical outcome in most cases. In addition, the adaptive response will be looked at in cases where it does meet the clinical performance requirements including animals that regenerate and for fetal wound healing. This will lead to how biomaterials can be used to alter the overall adaptive response to allow it to meet the desired clinical outcome. The important message of the review is that you need to use the engineering design process, not the scientific method, to design a clinical treatment. Also, the clinical performance requirements are functional, not structural. The last section will give some specific examples of controlling the adaptive response for two skin injuries: burns and pressure ulcers. For burns, it will cover some preclinical studies used to justify a clinical study as well as discuss the results of a clinical study using this system. For pressure ulcers, it will cover some preclinical studies for two different approaches: electrical stimulation and degradable/regenerative scaffolds. For electrical stimulation, the results of a clinical study will be presented.
Enhancement of Efficiency of a TiO2-BiFeO3 Dye-Synthesized Solar Cell through Magnetization
Authors: Kang Hyun Sik , Kim Woo Seoung , Kshetri Yuwaraj K. , Kim Hak Soo , Kim Hak Hee
Published: September 2022

Enhancement in the efficiency of a TiO2 dye-sensitized solar cell (DSSC) has been demonstrated by introducing ferromagnetic perovskite BiFeO3 and controlling the magnetic field, which induces two-dimensional material-like properties in the bulk of the TiO2-BiFeO3 DSSC (a 3-dimensional material). The effect of the concentration of BiFeO3 as well as the magnetization direction on the performance of the TiO2-BiFeO3 DSSC has been investigated. After magnetization, it was confirmed that the current density, efficiency, and open circuit voltage of the TiO2-BiFeO3 DSSC were increased. The observed phenomena have been explained in terms of the Hall effect which is responsible for the reduction of the degree of freedom of the electron movement resulting in the two-dimensional material-like properties in the bulk of the TiO2-BiFeO3 DSSC.
A Comparative Modelling Study of New Robust Packaging Technology 1 mm2 VCSEL Packages and Their Mechanical Stress Properties
Authors: Arshad Khairul Mohd , Noor Muhamad Mat , Manaf Asrulnizam Abd , Kawarada Hiroshi , Falina Shaili , Syamsul Mohd
Published: September 2022

Face recognition is one of the most sophisticated disciplines of biometric systems. The use of VCSEL in automotive applications is one of the most recent advances. The existing VCSEL package with a diffuser on top of a lens intended for automotive applications could not satisfy the criteria of the automotive TS16949: 2009 specification because the package was harmed and developed a lens fracture during 100 thermal cycle tests. In order to complete a cycle, the temperature rises from −40 °C to 150 °C and then rises again from 150 °C to 260 °C. The package then needs to be tested 500 times to ensure it fits the requirements without failing in terms of appearance or functionality. To this extent, the goal of this research is to develop packaging for 1 mm2 VCSEL chips with a diffuser on top that prevents fractures or damage to the package during heat cycle testing with multiple materials. The package was created using the applications SolidWorks 2017 and AutoCAD Mechanical 2017. The ANSYS Mechanical Structural FEA Analysis program simulated all packages for mechanical stress to guarantee that all packages generated were resilient to high temperature conditions. All packages exhibit no abnormalities and are robust for various temperatures ranging from low to high. Therefore, these packaged 1 mm2 VCSEL chips with a diffuser on top provide an effective approach for the application of VCSEL suitable in high temperature conditions.
The Forward and Lateral Tilt Angle of the Neck and Trunk Measured by Three-Dimensional Gait and Motion Analysis as a Candidate for a Severity Index in Patients with Parkinson’s Disease
Authors: Matsumoto Hirofumi , Shiraishi Makoto , Higashi Ariaki , Hino Sakae , Kaburagi Mayumi , Mizukami Heisuke , Maki Futaba , Yamauchi Junji , Tanabe Kenichiro , Sato Tomoo , Yamano Yoshihisa
Published: September 2022

(1) Objective: To evaluate the usefulness of a three-dimensional motion-analysis system (AKIRA®) as a quantitative measure of motor symptoms in patients with Parkinson’s disease (PD). (2) Method: This study included 48 patients with PD. We measured their motion during 2 m of walking using AKIRA®, we calculated the tilt angles of the neck and trunk, ankle height, and gait speed, then we compared these parameters with the MDS-UPDRS and the Hoehn and Yahr scale. Furthermore, we measured these AKIRA indicators before and after 1 year of observation. (3) Results: The forward tilt angle of the neck showed a strong correlation with the scores on parts II, III, and the total MDS-UPDRS, and the tilt angle of the trunk showed a moderate correlation with those measures. The lateral tilt angle of the trunk showed a moderate correlation with a freezing of the gait and a postural instability. Regarding changes over the course of 1 year (n = 34), the total scores on part III of the MDS-UPDRS and the forward tilt angle of the neck improved, while the lateral tilt angle of the trunk worsened. (4) Conclusion: Taken together, the forward and lateral tilt angles of the neck and trunk as measured by AKIRA® can be a candidate for quantitative severity index in patients with PD.
A Hot Water Extract of Curcuma longa L. Improves Fasting Serum Glucose Levels in Participants with Low-Grade Inflammation: Reanalysis of Data from Two Randomized, Double-Blind, Placebo-Controlled Trials
Authors: Uchio Ryusei , Okuda-Hanafusa Chinatsu , Saji Ryosuke , Kawasaki Kengo , Muroyama Koutarou , Murosaki Shinji , Yamamoto Yoshihiro , Hirose Yoshitaka
Published: September 2022

The dietary spice Curcuma longa L. (C. longa), also known as turmeric, has various biological effects. A hot water extract of C. longa was shown to have anti-inflammatory activities in preclinical and clinical studies. Chronic low-grade inflammation is associated with the disruption of glucose homeostasis, but the effect of C. longa extract on glucose metabolism in humans is poorly understood. Therefore, we investigated the effect of C. longa extracts on serum glucose levels in the presence of low-grade inflammation. We reanalyzed our published data from two randomized, double-blind, placebo-controlled trials in overweight participants aged 50 to 69 years and performed a stratified analysis using the inflammatory marker high-sensitivity C-reactive protein (hsCRP). In both studies, participants took a test food with a hot water extract of C. longa (C. longa extract group, n = 45 per study) or without C. longa extract (placebo group, n = 45 per study) daily for 12 weeks, and we measured the levels of serum hsCRP and fasting serum glucose. The mean baseline hsCRP value was used to stratify participants into two subgroups: a low-hsCRP subgroup (baseline mean hsCRP < 0.098 mg/dL) and a high-hsCRP subgroup (baseline mean hsCRP ≥ 0.098 mg/dL). In the low-hsCRP subgroup, we found no significant difference in fasting serum glucose levels between the two groups in either study, but in the high-hsCRP subgroup, the C. longa extract group had significantly lower levels of serum hsCRP (p < 0.05) and fasting serum glucose (p < 0.05) than the placebo group in both studies. In conclusion, a hot water extract of C. longa may help to improve systemic glucose metabolism in people with chronic low-grade inflammation.
Modifications of Chest CT Body Composition Parameters at Three and Six Months after Severe COVID-19 Pneumonia: A Retrospective Cohort Study
Authors: Besutti Giulia , Pellegrini Massimo , Ottone Marta , Bonelli Efrem , Monelli Filippo , Farì Roberto , Milic Jovana , Dolci Giovanni , Fasano Tommaso , Canovi Simone , Costi Stefania , Fugazzaro Stefania , Massari Marco , Ligabue Guido , Croci Stefania , Salvarani Carlo , Pattacini Pierpaolo , Guaraldi Giovanni , Giorgi Rossi Paolo
Published: September 2022

We aimed to describe body composition changes up to 6–7 months after severe COVID-19 and to evaluate their association with COVID-19 inflammatory burden, described by the integral of the C-reactive protein (CRP) curve. The pectoral muscle area (PMA) and density (PMD), liver-to-spleen (L/S) ratio, and total, visceral, and intermuscular adipose tissue areas (TAT, VAT, and IMAT) were measured at baseline (T0), 2–3 months (T1), and 6–7 months (T2) follow-up CT scans of severe COVID-19 pneumonia survivors. Among the 208 included patients (mean age 65.6 ± 11 years, 31.3% females), decreases in PMA [mean (95%CI) −1.11 (−1.72; −0.51) cm2] and in body fat areas were observed [−3.13 (−10.79; +4.52) cm2 for TAT], larger from T0 to T1 than from T1 to T2. PMD increased only from T1 to T2 [+3.07 (+2.08; +4.06) HU]. Mean decreases were more evident for VAT [−3.55 (−4.94; −2.17) cm2] and steatosis [L/S ratio increase +0.17 (+0.13; +0.20)] than for TAT. In multivariable models adjusted by age, sex, and baseline TAT, increasing the CRP interval was associated with greater PMA reductions, smaller PMD increases, and greater VAT and steatosis decreases, but it was not associated with TAT decreases. In conclusion, muscle loss and fat loss (more apparent in visceral compartments) continue until 6–7 months after COVID-19. The inflammatory burden is associated with skeletal muscle loss and visceral/liver fat loss.
Microbial Metabolism of Naringin and the Impact on Antioxidant Capacity
Authors: Zeng Xuan , Zheng Yuying , He Yan , Zhang Jiashuo , Peng Wei , Su Weiwei
Published: September 2022

Naringin is a dietary flavonoid glycoside with broad bioactivities, and it has been found to undergo extensive microbial metabolism in human gut. Microbial metabolites are believed to play an important role in the overall bioactivity of naringin. However, knowledge is scarce about its microbial metabolism in laboratory rats, which are the most commonly used animal model for naringin-related biomedical studies. Herein, we profiled the microbial metabolism of naringin in rat by an in vitro anaerobic fermentation combined with LC-MS/MS methods. A total of 35 microbial metabolites were identified, and corresponding metabolic pathways were proposed. Naringin and its metabolites were further quantified in fermentation samples. Rhoifolin, neoeriocitrin, neohesperidin, naringenin, methylated naringin, and hydroxylated naringin were detected as the primary microbial metabolites. Moreover, antioxidant capacity assays suggested that fermentation-associated microbial metabolites exhibited higher antioxidant activity than original naringin. Obtained results contribute to a more comprehensive understanding of the microbial metabolism and antioxidant capacity of naringin.
Maternal Vitamin B12 Deficiency Detected by Newborn Screening—Evaluation of Causes and Characteristics
Authors: Reischl-Hajiabadi Anna T. , Garbade Sven F. , Feyh Patrik , Weiss Karl Heinz , Mütze Ulrike , Kölker Stefan , Hoffmann Georg F. , Gramer Gwendolyn
Published: September 2022

Vitamin B12 deficiency, mostly of maternal origin in newborns, is a well-treatable condition but can cause severe neurologic sequelae in infants. Early detection of vitamin B12 deficiency allows the pre-symptomatic treatment of affected children. This evaluation assesses the characteristics of maternal vitamin B12 deficiency detected by newborn screening. In a prospective single-center study, a systematic screening strategy for vitamin B12 deficiency using a combination of two second-tier strategies was applied. In addition to confirmatory diagnostics in children, the systematic work-up of vitamin B12 status was also performed for their mothers. Maternal characteristics were assessed including ethnic origin, diet, and vitamin supplementation during pregnancy. For affected mothers, a work-up by internal medicine was recommended. In total, 121 mother–infant couples were analyzed. 66% of mothers adhered to a balanced diet including meat. The cause of maternal vitamin B12 deficiency was unknown in 56% of cases, followed by dietary causes in 32%, and organic causes in 8%. All mothers following a vegan diet and most mothers with a vegetarian diet took vitamin preparations during pregnancy, whereas only 55.8% of mothers with a balanced diet took folic acid or other vitamins. Maternal vitamin B12, folic acid, and homocysteine levels were significantly correlated with the child’s folic acid levels, and with homocysteine, methylmalonic, and methylcitric acid levels in first and second NBS dried blood spots. Most children had normal blood counts and showed normocytosis. Although 36.7% of mothers showed anemia, only one presented with macrocytosis. Adherence to vitamin supplementation in pregnancy is low despite the recommendation for supplementation of folic acid. Ideally, the evaluation of mothers for vitamin B12 levels and appropriate therapy should be initiated in early pregnancy. In infants detected through newborn screening, the multidisciplinary assessment and therapy of both children and mothers should be performed.
Effects of Genistein on Common Kidney Diseases
Authors: Peng Qianwen , Li Yuanyuan , Shang Jia , Huang Haitao , Zhang Yiming , Ding Yueming , Liang Yipei , Xie Zhenxing , Chen Chaoran
Published: September 2022

Genistein is a naturally occurring phytoestrogen (soy or soybean products) that is classified as an isoflavone, and its structure is similar to that of endogenous estrogens; therefore, genistein can exert an estrogen-like effect via estrogen receptors. Additionally, genistein is a tyrosine kinase inhibitor, which enables it to block abnormal cell growth and proliferation signals through the inhibition of tyrosine kinase. Genistein is also an angiogenesis inhibitor and an antioxidant. Genistein has effects on kidney cells, some of the kidney’s physiological functions, and a variety of kidney diseases. First, genistein exerts a protective effect on normal cells by reducing the inflammatory response, inhibiting apoptosis, inhibiting oxidative stress, inhibiting remodeling, etc., but after cell injury, the protective effect of genistein decreases or even has the opposite effect. Second, genistein can regulate renin intake to maintain blood pressure balance, regulate calcium uptake to regulate Ca2+ and Pi balances, and reduce vasodilation to promote diuresis. Third, genistein has beneficial effects on a variety of kidney diseases (including acute kidney disease, kidney cancer, and different chronic kidney diseases), such as reducing symptoms, delaying disease progression, and improving prognosis. Therefore, this paper reviews animal and human studies on the protective effects of genistein on the kidney in vivo and in vitro to provide a reference for clinical research in the future.