bluuebunny/crossref_metadata_2025 huggingface dataset:
COVERT SIMULTANEOUS POST-EDITING IN ONLINE ASSESSMENT OF STUDENTS’ SIGHT TRANSLATION
Authors: Chernovaty Leonid Mykolayovych
Published: September 2024

This first attempt aims to determine the extent of students’ covert use of machine translation (MT) in the online assessment of their sight translation, the strategies of such use, and its signs. The study is based on the analysis of target texts (TT) of specialised online sight translation from Ukrainian into English by 13 BA and 10 MA students. The procedure involved the comparison of the students' TTs with their MT counterparts. Signs of covert MT were found in 46% of the BA and 30% of the MA students’ translations. The main method of this covert MT use is "simultaneous post-editing", i.e., the immediate oral post-editing of the MT text generated by the students on their screens and hidden from the assessor, while they deliver their supposedly original TTs. Simultaneous post-editing strategies range from replacing individual lexemes with their synonyms, adding and deleting elements, changing the syntactic functions of words or phrases, rearranging sentence fragments, transforming their structure, to applying several of these strategies simultaneously. Other methods of concealment include alternating MT systems in translating the same source text, as well as artificially slowing down the process of reading the TT from the screen, accompanied by pauses in the relevant text fragments to perform certain mental operations. In order to increase objectivity, the author recommends a delayed assessment of students' online interpreting recordings. The research perspective is to study the didactic potential of simultaneous post-editing as a procedure for developing general interpreting skills.
PROJECT MANAGEMENT PEDAGOGY: CULTIVATING CRITICAL THINKING SKILLS IN HIGHER EDUCATION
Authors: Attipoe Sonny Gad
Published: September 2024

The present study explores the integration of critical thinking skills concepts in agricultural project management instruction at the undergraduate level, using the traditional classroom theoretical approach and a blended learning model. The study, encompassing 118 undergraduate students, compares the effectiveness of these methods in enhancing students' understanding, measured through examination scores, group presentations, and students’ overall perceptions of the use of the methods. Students achieved a mean examination score of 64.82% under the traditional approach and 72.66% for the blended learning approach. Statistical analysis, including a t-test, revealed significant differences favoring the blended approach. Additionally, performance assessments using six criteria during presentations yielded varied mean scores. ANOVA analysis showed a significant difference among the groups, and post hoc tests highlighted distinctions between specific groups’ means, emphasizing the effectiveness of blended learning in enhancing students understanding. Students' perceptions, gathered through a 5-point Likert scale, underscored satisfaction with the blended approach due to its flexibility, catering to diverse learning styles, and fostering critical thinking skills. We recommend institutions strengthen project management instruction by adopting blended learning, emphasizing critical thinking, and prioritizing continuous assessment and student-centric approaches since they cultivate an engaging learning experience, preparing students for managing future complexities on projects.
GENDER DIFFERENCES IN BUSINESS TRAVEL: IMPLICATIONS FOR HOTEL AND DESTINATION MANAGEMENT
Authors: MILLÁN Ángel , FANJUL M. Luisa , RETAMOSA Marta
Published: September 2024

Purpose – The aim of this study is to investigate the influence of gender differences on the evaluation of hotels and destination attributes with regard to business travel. Methodology/Design/Approach – This research employs a cross-sectional questionnaire survey and a sample of 318 business travellers. The analysis methods used are descriptive, such as a t-test, frequency distribution, cross tabulation and a chi-square analysis. Findings – The findings reveal significant differences when evaluating the attributes of hotels and destinations. Certain hotel attributes are rated at a significantly higher level by women than by men. Female respondents attained higher mean scores for all the destination attributes. Previous studies have attained very little knowledge in terms of understanding how gender works as regards differentiating business travellers’ behaviour. Originality of the research – The results support the hypothesis that gender influences the evaluation of hotel services and destinations. These findings may have significant implications for service innovation and positioning strategies in the business travel industry.
SERVANT LEADERSHIP AND EMPLOYEE CREATIVITY: THE MEDIATING ROLE OF HARMONIOUS PASSION AND PSYCHOLOGICAL SAFETY
Authors: YULIANEU Aneu , YUSUF Muhamad Nurdin
Published: September 2024

Purpose - This paper examines simultaneously mediating mechanisms through which servant leadership (SL) affects employee creativity (EC). Specifically, the authors test harmonious passion (HP) and psychological safety (PS) as the mediating paths through which servant leadership predicts employee creativity. Design/Methodology/Approach - Data were collected from 274 employees in seven four- and five-star hotels in Indonesia. A partial least squares structural equation modelling technique was used to estimate the measurement and structural models. Findings -The findings of this study indicate that managers should adopt servant leadership behaviors, which can improve employees’ psychological resources, such as harmonious creativity, passion, and psychological safety. Originality of the research - This study offers original insights into the emerging body of hospitality literature by presenting the initial empirical proof of the combined impact of harmonious passion and psychological safety in facilitating the influence of servant leadership on frontline employees’ creativity.
Harmonizing Quantum Gravity with Thermodynamics: Determination of the EUP Parameter by Prigogine’s Law
Authors: Sonnino Giorgio
Published: September 2024

In 1974, Stephen Hawking made the groundbreaking discovery that black holes emit thermal radiation, characterized by a specific temperature now known as the Hawking temperature. While his original derivation is intricate, retrieving the exact expressions for black hole temperature and entropy in a simpler, more intuitive way without losing the core physical principles behind Hawking's assumptions is possible. This is obtained by employing the Heisenberg uncertainty principle which is known to be connected to the the vacuum fluctuation. This exercise allows us to easily perform more complex calculations involving the effects of quantum gravity. This work aims to answer the following question: \textit{Is it possible to reconcile Prigogine's second law of thermodynamics for open systems and the second law of black hole dynamics with Hawking radiation}? Due to the effects of quantum gravity, the Heisenberg uncertainty principle has been extended to the Generalized Uncertainty Principle (GUP) and successively to the Extended Uncertainty Principle (EUP). The expression for the EUP parameter is obtained by conjecturing that Prigogine's second law of thermodynamics and the second law of black holes are not violated by Hawking thermal radiation mechanism.
Constructing the Early-Stage Framework of Cultural Identity Enlightenment in Heritage Education
Authors: Zheng Ran , Guo Liangjing
Published: September 2024

Education and heritage are fundamental to achieving sustainable development goals. Integrating heritage into education is crucial for cultivating children's connection to heritage and promoting sustainable development. However, the lack of a scientific framework to guide educational goals across age groups results in unclear integration. This study aims to develop a framework for cultural identity enlightenment within heritage education by conducting literature research and validating it through field observations in kindergartens. It identifies cognitive, emotional and behavioral dimensions, outlining the developmental trajectory of these goals. This framework illustrates how children's understanding progresses from direct experiences to deeper appreciation and creative involvement. It provides teachers with a tool to set goals that empower children as agents, fostering the emergence of cultural awareness and identity. This approach not only enhances cultural identity but also supports sustainable development by advancing the transmission and innovation of cultural heritage.
Combined Epiretinal Proliferation and Internal Limiting Membrane Inverted Flap for the Treatment of Large Macular Holes
Authors: Dervenis Nikolaos , Vagiakis Iordanis , Papadopoulou Elena P , Dervenis Panagiotis , Sandinha Maria Teresa
Published: September 2024

We are presenting a new method for the treatment of large macular holes (MH) with the use of an inverted flap consisting of both internal limiting membrane (ILM) and epiretinal proliferation (EP). A prospective interventional case series was conducted from September 2021 to January 2023. MH patients with coexistent EP visualized preoperatively in macula optical coherence tomography and MH minimum linear diameter larger than 400 microns underwent standard pars plana vitrectomy with the creation of an inverted petaloid flap (consisting of both ILM and EP) and gas tamponade. 16 eyes were included in our study. MH closure was successful in all the eyes with a single procedure. Preoperative minimum linear diameter was 707.63(±164.02μm) and preoperative best corrected visual acuity was 1.11±0.52. Postoperative BCVA was 0.51±0.20 (p=0.01) at 6weeks postoperatively and final BCVA was 0.45±0.20 (p=0.008). EP can be safely combined with ILM for the creation of an inverted, petaloid flap to cover and facilitate the closure of large MH.
Perceptions of Cancer through the Ages – from Hippocratic Oncology to Precision Cancer Medicine
Authors: Retsas Spyros
Published: September 2024

The unravelling of the human genome created new perceptions on the origin and evolution of diseases and for cancer in particular, it established the notion that neoplasia has been a companion of life since its ap-pearance on earth.
It is not surprising that neoplasms, in various forms, develop in numerous species of animals and even in plants. Unmistakable accounts of cancer with clinical features as are understood today, begin in the 5th c. B.C. The principles and practice of the Hippocratic and Galenic tradition dominated cancer care virtually into the 20th century. Advanced sequencing technologies at the dawn of the 21st century, generate new ther-apeutic opportunities with immunotherapy, oncolytic virotherapy and gene transfer, the latter especially in cases of hereditary cancer.
Evaluation of the Impact of Advanced Glycation End-Products on Peripheral Neuropathy Outcomes in Type 2 Diabetes
Authors: Singh Indumathi , Issar Tushar , Poynten Ann , Milner Kerry-lee , Krishnan Arun V , Dhanapalaratnam Roshan
Published: September 2024

Background: Type 2 diabetes mellitus (T2DM) affects over 500 million people worldwide, and over 50% of this group experience the most common complication, diabetic peripheral neuropathy (DPN). The presence of advanced glycation end-products (AGEs) have been linked with the development of DPN. The present study assessed for AGE levels in participants with type 2 diabetes, and explored the hypothesis that there may be increased AGE levels in more severe DPN.
Methods: 124 participants with T2DM were consecutively recruited, and underwent skin autofluorescence, clinical assessment for peripheral neuropathy, peripheral nerve ultrasound, nerve conduction studies and axonal excitability assessment.
Results: Elevated levels of AGE did not correlate with higher clinical neuropathy scores, sural sensory nerve amplitude, median or tibial nerve cross-sectional area or measures of axonal excitability. While higher AGE readings correlated with diabetic kidney disease, these recordings were not associated with more severe peripheral neuropathy outcomes on multiple regression analysis.
Conclusion: The present study suggests that accumulation of AGE is not associated with clinical, electrophysiological and morphological measures of neuropathy in T2DM.
Dual Fractions Proteomic Analysis of Silica Nanoparticles Interactions with Protein Extracts
Authors: Schvartz Marion , Saudrais Florent , Boulard Yves , Renault Jean-Philippe , Henry Céline , Chédin Stéphane , Pin Serge , Aude Jean-Christophe
Published: September 2024

Dual-fraction proteomics reveals a novel class of proteins impacted by nanoparticle exposure. Background: Nanoparticles (NPs) interact with cellular proteomes, altering biological processes. Understanding these interactions requires comprehensive analyses beyond solely characterizing the NP corona. Methods: We utilized a dual-fraction mass spectrometry (MS) approach analyzing both NP-bound and unbound proteins in Saccharomyces cerevisiae sp. protein extracts exposed to silica nanoparticles (SiNPs). We identified unique protein signatures for each fraction and quantified protein abundance changes using spectral counts. Results: Strong correlations were observed between protein profiles in each fraction and non-exposed controls, while minimal correlation existed between the fractions themselves. Linear models demonstrated equal contributions from both fractions in predicting control sample abundance. Combining both fractions revealed a larger proteomic response to SiNP exposure compared to single-fraction analysis. We identified 302/56 proteins bound/unbound to SiNPs and an additional 196 "impacted" proteins demonstrably affected by SiNPs. Conclusion: This dual-fraction MS approach provides a more comprehensive understanding of nanoparticle interactions with cellular proteomes. It reveals a novel class of "impacted" proteins, potentially undergoing conformational changes or aggregation due to NP exposure. Further research is needed to elucidate their biological functions and the mechanisms underlying their impact.
Assessment of the Correlation between Unipolar and Bipolar Depression, Calcium Levels, Thyroid Function, and Inflammatory Markers: A Pilot Prospective Study
Authors: Ielmini Marta , Gallo Daniela , Tanda Maria Laura , Piantanida Eliana , Callegari Camilla , Caselli Ivano
Published: September 2024

Recent mental health research has highlighted inflammation's critical role in conditions like depression and bipolar disorder, examining correlations with markers such as calcium levels, thyroid function, and inflammatory markers. The study aims to compare unipolar and bipolar d depression samples in terms of socio-demographic and clinical features. Prospective enrollment of patients hospitalized at the University of Insubria Psychiatric Unit. Patients were administered psychometric questionnaires (Beck Depression Inventory-II, Mental Pain Questionnaire, Beck Anxiety Inventory, Clinical Global Impressions Severity scale, and Reasons for Living Inventory). Thirty-six patients (17 males, 19 females) were enrolled. A statistically significant association emerged between calcium levels and bipolar depression (Pearson Chi square=0.050) and between CRP and bipolar depression (Pearson Chi-square=0.017). Additionally, a statistically significant association was observed between the unipolar and bipolar depression subgroups and current substance abuse (p=0.0275) A statistically significant difference between the BRLF scores and UDD subgroup emerged (p=0.0126). The results of the pilot study revealed significant associations between calcium/CRP levels with depressive symptoms, particularly in bipolar depression. Further research on inflammatory markers is needed to refine diagnostic subtypes and therapeutic strategies.
A Scoping Review of Genetic Algorithms in Serious Games: Applications, Challenges, and
Future Directions
Authors: Vaghani Vrushank , Oyibo Kiemute
Published: September 2024

The integration of Genetic Algorithms (GAs) into Serious Games (SGs) has gained traction
as a method to optimize game mechanics and personalize user experiences. While GAs are
well-known for their effectiveness in solving complex optimization problems, their application in
SGs remains relatively under-explored. This review aims to address this gap by systematically
mapping the existing literature on the intersection of these two fields. The primary objective
of this scoping review is to identify and synthesize the existing research on the application of
GAs in SGs, with a focus on understanding the current state of the field, identifying common
applications, challenges, and potential future directions. The review includes studies published in
English that focus on the use of GAs within SGs. The search encompassed articles from academic
journals and conference proceedings without restrictions on publication dates. Exclusion criteria
included studies that did not specifically address the integration of GAs in SGs. A systematic
search was conducted in databases such as ACM Digital Library, Web of Science, Scopus
and Inspec in July 2024. The search terms used included "genetic algorithm," "evolutionary
algorithm," "computational algorithm," and "serious games." Unpublished data and articles
were not considered. Data were charted using a PRISMA flowchart developed by the author
VV. Key categories for data extraction included study objectives, methods, outcomes, type of
study and GA used and the specific applications of GAs in SGs. Data charting was conducted
independently by two reviewers, with disagreements resolved through discussion and consensus.
The search identified 154 studies, of which 23 met the inclusion criteria. The included studies
highlight the diverse applications of GAs in SGs, ranging from optimizing game scenarios to
personalizing learning experiences. Challenges identified include the computational complexity
of GAs and difficulties in their integration into existing game frameworks. The findings of this
review suggest that while the use of GAs in SGs is a promising area of research, it remains in its
nascent stages. Future research should focus on addressing the technical challenges of integrating
GAs into SGs and exploring their application across a wider range of game genres and educational
contexts. Limitations of the review include the potential for publication bias and the exclusion of
non-English language studies.
Early Enteral Nutrition in Patients Undergoing Intestinal Anastomosis
Authors: Toro Carlos
Published: September 2024

A prospective longitudinal study of 54 patients who underwent intestinal anastomosis and were started on early enteral nutrition at the University Hospital of the Central University of Venezuela (November 2022 to September 2023), all of them fulfilled the inclusion criteria. Taking as early enteral nutrition two study groups: GROUP 1 tolerance at 24 hours and GROUP 2. Tolerance at 48 hours. Hospital stay and complications were evaluated. Of the 54 patients 40(74.1%) were operated on in emergency surgery 46(85.2%) patients were male. There was no difference in the mean ages of the two diet groups (P=0.254), the mean age was 33.9/14.8 years. There was no statistically significant difference (P=0.086) in the mean number of days of hospitalization (4.7/2.4 days) between patients according to the type of diet. Vomiting occurred in 20.37%, with no statistically significant differences between the groups of patients with diet 1 and 2. There were 2 (3.7%) cases of leakage that required reintervention, and this complication was not associated with the initiation of a particular type of diet, type of surgery, surgical time or site of injury. Early enteral nutrition is a viable option in patients undergoing intestinal anastomosis, is not associated with the incidence of dehiscence, is well tolerated by the patients, has acceptable complications, most frequently associated with vomiting, and reduces hospital stay.
The Distribution of Prime Numbers Based on the Proof of Riemann’s Hypothesis and the Properties of the Numbers 3, 6, and 9
Authors: mousavi seyed kazem
Published: September 2024

In this paper, the distribution of prime numbers is expressed based on proving the Riemann hypothesis. The modality to the distribution of prime numbers is one of the most important results of proving the Riemann hypothesis. The relationship between three numbers, three, six, and nine, and the modality to the distribution of prime numbers, is one of the results of Riemann's zeta function. Prime numbers are classified into six groups of single-digit numbers. There are no prime numbers in groups of three, six, and nine. The groups are made based on the sum of the internal digits. And for each set, there is an angle in the complex plane. The distance between the prime numbers in each group has a regular pattern. This pattern is a multiple of the numbers three, six, and nine. According to Euler's number, for an angle of 60 degrees, the real part of the cosine is 0.5. Accordingly, all prime numbers are related to angles greater than 60 degrees to 90 degrees. As a result, based on the relationship between the golden spiral and the complex conjugate of the zeta function, the function in The 1/2 point becomes zero.
The Current Landscape of Clinical Trails
Authors: Joshi Geeta , Bhandari Tara K. , Joshi Pushkar , Bhandari Smriti , Araveeti Shalini Reddy , Jain Aditi , Khadka Subash , Trecarten Shaun , Abdelaziz Ahmad , Garg Harshit , Bhandari Mukund
Published: September 2024

Clinical trials are essential in the development of new medical treatments, offering crucial data on their safety and effectiveness. This study provides a comprehensive analysis of clinical trials registered on ClinicalTrials.gov, examining trends, challenges, and innovations that have shaped the field over the past century. Data were extracted on July 15, 2024, and analyzed to identify patterns in trial design, sponsorship, participant demographics, and geographical distribution. The analysis reveals a significant increase in clinical trial registrations, peaking in 2021, driven by the COVID-19 pandemic. Most trials focus on cancer, reflecting its global burden, and are predominantly interventional, with randomized controlled trials (RCTs) being the most common study design. However, challenges persist, including underrepresentation of certain demographics, limited global distribution, and insufficient reporting of trial results. Additionally, the underrepresentation of pediatric, elderly, and minority populations in trials limits the generalizability of findings. The analysis underscores the need for more inclusive and globally distributed research to address disparities in health outcomes.
Effectiveness of Integrated Vestibular Rehabilitation in Improving Balance and Motor Function in Patients with Benign Paroxysmal Positional Vertigo
Authors: Crișan-Mălăncrăvean Patricia-Maria , Brînzaniuc Klara , Mühlfay Gheorghe
Published: September 2024

This study evaluates the effectiveness of an integrated vestibular rehabilitation program in improving balance, motor control, and proprioceptive function in patients diagnosed with Benign Paroxysmal Positional Vertigo (BPPV). The prospective observational study involved 107 participants who underwent a tailored rehabilitation regimen, including the Epley maneuver, Brandt-Daroff exercises, and balance and proprioceptive training. The program was designed to address the vestibular and proprioceptive deficits caused by BPPV, with the goal of restoring normal function and reducing the risk of symptom recurrence. Dynamic Gait Index (DGI) and Balance Beam Test were used to assess progress, showing significant improvements in balance stability and dynamic motor function. The findings support the integration of vestibular rehabilitation into standard care for BPPV patients, highlighting the program's potential to enhance patient outcomes and reduce recovery time. However, the study also notes limitations such as the lack of a control group and the need for further research to explore the long-term effects of such interventions.
Lignans and other components of phytoextracts for the treatment of fibrocystic mastopathy
Authors:   Ivanovo State Medical University, Ivanovo, Russian Federation, Rubashkina А.N. , Gromova O.A. ,   Federal Research Center «Computer Science and Control», RAS, Moscow, Russian Federation, Torshin I.Yu. ,   Federal Research Center «Computer Science and Control», RAS, Moscow, Russian Federation
Published: September 2024

Objective. Comparative analysis of data on various plant extracts and their components, characterized by antitumor and antiinflammatory effects. Materials and methods. Systematic data analysis using topological recognition theory methods, clinical study of patients with fibrocystic mastopathy (FCM). Results. The analysis showed that the anti-inflammatory, antioxidant and antitumor properties of extracts of oleander, hauttuynia cordata, mistletoe, bitter melon, ginkgo biloba, grape seeds, cohosh, chasteberry and common spruce may be useful in the treatment of mastopathy and the prevention of breast cancer. A new class of candidate molecules with special antitumor properties, resinol lignans, has been isolated from Norway spruce extracts. In particular, 7-hydroxymatairesinol can be used for FCM, accompanied by various manifestations of menopausal syndrome, incl. mastalgia. The presented results of the original clinical study showed that during therapy with FCM 7-hydroxymatairesinol, there was a decrease or complete disappearance of menopausal syndrome, pain in the mammary glands and other symptoms. Positive dynamics of estrogen metabolites, their ratios and blood lipid profile indicators were noted, indicating normalization of estrogen metabolism. According to ultrasound data, after therapy with 7-hydroxymatairesinol, a decrease in the number and size of cystic formations in FCM was noted. Conclusion. Despite the fact that lignans from spruce extracts are the least studied, this group of compounds is promising for use in FCM to normalize estrogen metabolism and reduce fibrocystic changes in the mammary glands. Key words: phytoextracts, fibrocystic mastopathy, mastalgia, menopausal syndrome, 7-hydroxymatairesinol
Oxidative stress indices and endothelial dysfunction in type 2 diabetes: risks and focus on diet therapy
Authors:   Federal Research Centre of Nutrition, Biotechnology and Food Safety, Moscow, Russian Federation, Alekseev V.A. , Sharafetdinov Kh.Kh. ,   Federal Research Centre of Nutrition, Biotechnology and Food Safety, Moscow, Russian Federation,   Russian Medical Academy of Continuous Professional Education, Moscow, Russian Federation,   I.M.Sechenov First Moscow State Medical University (Sechenov University), Moscow, Russian Federation, Plotnikova O.A. ,   Federal Research Centre of Nutrition, Biotechnology and Food Safety, Moscow, Russian Federation, Vorozhko I.V. ,   Federal Research Centre of Nutrition, Biotechnology and Food Safety, Moscow, Russian Federation
Published: September 2024

Type 2 diabetes (T2D) is a chronic metabolic disease that poses a significant threat to public health worldwide. Carbohydrate metabolism disorders in T2D are closely related to oxidative stress. Numerous vascular complications are the main cause of disability and mortality in this disease. Objective. Comparative evaluation of the indices of oxidative stress and endothelial dysfunction with anthropometric and biochemical parameters in T2D patients. Patients and methods. The study included 55 patients with T2D and 58 patients with class 1–3 obesity without diabetes. Patients with T2D received a standard treatment with oral sugar-lowering drugs, hypolipidemic and hypotensive therapy as indicated. All patients underwent measurements of anthropometric parameters (height, weight, body mass index), indices of carbohydrate and lipid metabolism, antioxidant status and endothelial dysfunction, triglycerides-glucose index, and pulse wave velocity. Statistical processing of the results was performed using SPSS 23.0 software. Results. Several correlations were found between oxidative stress indices and clinical and metabolic parameters characterizing the state of carbohydrate and lipid metabolism and anthropometric indices in patients with T2D. The level of high-sensitivity C-reactive protein was almost twice as high in T2D patients compared to patients with obesity (11.1 ± 2.58 mg/L and 6.77 ± 1.11 mg/L, respectively). A negative correlation between the levels of superoxide dismutase and fasting blood glucose was determined (r = -0.30, p < 0.005). Conclusion. The study revealed a strong correlation between oxidative stress indices, markers of endothelial dysfunction and cardiometabolic disorders in patients with T2D. The influence on oxidative stress/antioxidant defense indices may become an additional therapeutic link in the treatment of T2D patients. Key words: reactive oxygen species, endothelial dysfunction, oxidative stress, type 2 diabetes
Rancang Bangun Sistem Informasi Kelola Magang (SIKAM) di LPTP Surakarta
Authors: Yudhanto Yudho , Albab Hypatia Ulil
Published: September 2024

Abstrak 

LPTP telah memiliki website yang berfungsi sebagai wadah informasi yang berguna untuk menyampaikan informasi terkait LPTP kepada masyarakat. Saat ini, proses update artikel yang dibuat dari luar masih lama dikarenakan harus melalui email  kepada admin. Permasalahan berikutnya adalah tentang proses pengajuan magang di LPTP yang masih manual manual dan harus offline dikombinasikan menggunakan email. Sehingga menimbulkan permasalahan yang ketiga yakni sulitnya memantau kinerja peserta magang yang belum tersimpan didalam database, akibatnya banyak data peserta magang yang tidak terarsip. Solusi yang diusulkan adalah dengan membuat sebuah sistem informasi yang digunakan untuk mengelola data artikel dan data peserta magang. Aktor yang terlibat adalah admin, pegawai dan masyarakat. Perancangan dan Pembuatan Sistem Informasi Kelola Magang (SIKAM) dibuat dengan perancangan analisis permodelan Bisnis Proses, Kebutuhan Perangkat Lunak, Use Case Diagram, Activity Diagram. Dengan metode Prototyping maka digunakanlah bahasa pemrograman PHP dengan menggunakan framework CodeIgniter 3, dan perancangan basis data dengan menggunakan permodelan Entity Relationship Diagram. Untuk penyimpanan menggunakan basis data MySQL. Kesimpulan dari pengujian menunjukkan bahwa dari 34 skenario yang dijalankan dengan metode Black box didapatkan hasil berjalan dengan baik dan memuaskan.

================

Abstract

LPTP already has a website that functions as an information forum that is useful for conveying information related to LPTP to the public. Currently, the process of updating articles made from outside still takes a long time because they have to be emailed to the admin. The next problem is the process of applying for internships at LPTP which is still manual and must be offline combined using email. So that raises the third problem, namely the difficulty of monitoring the performance of apprentices who have not been stored in the database, as a result, a lot of apprentice data is not archived. The proposed solution is to create an information system that is used to manage article data and apprentice data. The actors involved are admin, employees and the community. The design and manufacture of Intern Management Information Systems (SIKAM) is made by designing business process modeling analysis, Software Requirements, Use Case Diagrams, Activity Diagrams. With the Prototyping method, the PHP programming language is used using the CodeIgniter 3 framework, and database design using the Entity Relationship Diagram model. For storage using MySQL database. The conclusion of the test shows that of the 34 scenarios that were run using the Black-box method, the results went well and were satisfactory.
Psikolojik Sağlamlık ile Kariyer Kaygısı Arasındaki İlişkinin İncelenmesi: Öğrenciler Üzerine Bir Uygulama
Authors: Aksu Muharrem , Kuas Yahya
Published: September 2024

Günümüzde öğrencilerin kariyer planlamalarını kaygıdan uzak bir şekilde yapabilmeleri sağlıklı bir kariyer karar alma süreci için gereklidir. Öğrencilerin başarılı bir kariyer süreci geçirebilmelerinde ise psikolojik sağlamlık önemli bir değişken olarak işlev görmektedir. Bu bağlamda çalışma, meslek yüksekokulu öğrencilerinin psikolojik sağlamlıklarının kariyer kaygılarını ne düzeyde etkilediğini ve her iki değişkenin cinsiyete göre farklılık gösterip göstermediğini ölçme amacıyla yapılmıştır. Araştırmada betimsel özelliğe sahip ilişkisel tarama modeli kullanılmıştır. Anket tekniğiyle 241 katılımcıdan veriler toplanmış ve SPSS programı yardımıyla analiz edilmiştir. Analiz sonuçlarına göre, öğrencilerin orta düzeyde psikolojik sağlamlığa ve kariyer kaygısına sahip oldukları tespit edilmiştir. Ayrıca psikolojik sağlamlık ile kariyer kaygısı arasında istatistiksel olarak negatif yönde anlamlı bir ilişki olduğu ve psikolojik sağlamlığın kariyer kaygısı üzerinde negatif yönlü bir etkisinin olduğu görülmüştür. Yani öğrencilerin psikolojik sağlamlıkları arttıkça kariyer kaygıları azalmaktadır. Cinsiyet bazında ise erkek öğrencilerin psikolojik sağlamlıklarının kadınlardan yüksek olduğu, kariyer kaygısı açısından ise cinsiyetler arasında farklılık olmadığı saptanmıştır.
Fragment based discovery of a small molecule RhoGDI2 ligand HR3119 that inhibits cancer cell metastasis
Authors: Ruan Ke , Liu Mingqing , Wan Shizhang , Guo Shangxi , Liu Jiuyang , Li Wenqian , Wang Lei , Li Fudong , Hai Zhang , Liu Xing , Liu Dan , Gao Jia , He Wei , Yao Xuebiao
Published: September 2024

Guanine nucleotide dissociation inhibitors (GDIs) proteins including RhoGDI2 play a central role in regulating the functions of Ras superfamily proteins that are known to be important cancer drug targets. Given the known challenges in directly targeting Ras superfamily proteins with small compounds, targeting GDIs represents a unique opportunity but has met with limited success. Herein, we describe the discovery of the first small compound HR3119 that binds RhoGDI2 in low micromolar affinity (Kd ~ 7 μM) starting from a millimolar binding affinity fragment hit (Kd ~ 0.7 mM) through iterative structure-guided design. We identify that HR3119 surrogates occupy the binding interface between RhoGDI2 and its endogenous ligand Rac1 in a series of co-crystal structures, laying out rich information for future compound design. We further demonstrate that HR3119 disrupts RhoGDI2-Rac1 binding and promotes the degradation of Rac1, as well as engages RhoGDI2 in cells. As an illustration of this novel chemical probe, we show that HR3119 suppressed the invasion and migration of invasive breast cancer cells at a 5 μM concentration and in a concentration-dependent manner. Interestingly, (6R)-HR3119 exhibited a 100-fold binding affinity advantage to that of (6S)-HR3119 (4 μM vs 290 μM) and improved anti-metastatic activity compared to racemic HR3119. This work provides valuable insights into the discovery of small compounds targeting GDIs in terms of methodology, chemistry starting points, compound design, and phenotype studies, underscoring exciting new perspectives in early drug discovery.
Evaluation of Positioning Accuracy and Clinical Outcomes in 3D Printing- Assisted Ankle Ligament Surgery
Authors: Luo Kai , Xiang Yi , Lu Kang , Du zhongbao , Ren Yanmei , Sun Liqin , Zhang Jian , Qiao Wenjie , Tang Xiangyu
Published: September 2024

Background: To investigate the positioning accuracy and clinical efficacy of 3D-printed, personalized guide plate assisted lateral ankle ligament reconstruction.
Methods: We retrospectively studied 48 patients with chronic lateral ankle instability and divided them into two groups: the template group (24 patients) and the conventional group (24 patients). The operation time, intraoperative X-ray exposure time, American Orthopedic Foot and Ankle Society and Foot and Ankle Outcome Score (FAOS) were compared between the two groups.
Results: All patients were followed up for 12-20 months (14.5 ± 5.5). The operation time in the template group was 32-55 min (38.6 ± 9.7), the operation time in the conventional group was 48-73 min (58.3 ± 12.6), and there was a significant difference between the two groups (P<0.05). The number of X-ray exposures in the template group was 1-3 (1.2 ± 0.6), the number of X-ray exposures in the conventional group was 3-6 (3.8 ± 2.2), and there was a significant difference between the two groups (p<0.05). There was no significant difference in the AOFAS score or FAOS score between the two groups before the operation orat the last follow-up after the operation. There was no significant difference in the talar anteversion distance or talar varus angle between the two groups at the last follow-up after the operation.
Conclusion: Comparedwith conventional surgery, 3D-printedtemplate surgery has a shorter operation time, higher positioning accuracy, and shorterradiation exposure time. 3D printing template surgery is a successful treatment option with the same satisfactory effect on patients with chronic lateral ankle instability.
Expression of anti-amyloid CARs in microglia promotes efficient and selective phagocytosis of Aβ1‒42
Authors: Heiss Christina , Riise Rebecca , Hanse Eric , Fruhwürth Stephanie , Zetterberg Henrik , Björefeldt Andreas
Published: September 2024

Genetic engineering of microglial cells is a promising therapeutic avenue emerging with advancements in gene delivery techniques. Using a recently developed AAV capsid for efficient in vitro transduction we report the engineering of microglia with CARs (CAR-Mic) targeting phagocytosis of amyloid beta 1‒42 (Aβ42). Functional screening of seven CAR constructs in human iPSC-derived microglia revealed up to 6-fold increases in internalized Aβ relative to viral control. CAR-driven phagocytic enhancement was selective for Aβ, dependent on intracellular domain signaling, and was confirmed in primary mouse microglia. These findings highlight the potential of using this approach to target dysfunctional microglia in Alzheimer’s disease and other CNS disorders.
Reversal Gene Expression Assessment for Drug Repurposing, a Case Study of Glioblastoma
Authors: Sun Shixue , Shyr Zeenat , McDaniel Kathleen , Fang Yuhong , Tao Dingyin , Chen Catherine Z. , Zheng Wei , Zhu Qian
Published: September 2024

Glioblastoma (GBM) is a rare brain cancer with an exceptionally high mortality rate, which illustrates the pressing demand for more effective therapeutic options. Despite considerable research efforts on GBM, its underlying biological mechanisms remain unclear. Furthermore, none of the United States Food and Drug Administration (FDA) approved drugs used for GBM deliver satisfactory survival improvement. This study presents a novel computational pipeline by utilizing gene expression data analysis for GBM for drug repurposing to address the challenges in rare disease drug development, particularly focusing on GBM. The GBM Gene Expression Profile (GGEP) was constructed with multi-omics data to identify drugs with reversal gene expression to GGEP from the Integrated Network-Based Cellular Signatures (iLINCS) database. We prioritized the candidates via hierarchical clustering of their expression signatures and quantification of their reversal strength by calculating two self-defined indices based on the GGEP genes’ log2 foldchange (LFCs) that the drug candidates could induce. Among eight prioritized candidates, in-vitro experiments validated Clofarabine and Ciclopirox as highly efficacious in selectively targeting GBM cancer cells. The success of this study illustrated a promising avenue for accelerating drug development by uncovering underlying gene expression effect between drugs and diseases, which can be extended to other rare diseases and non-rare diseases.
Novel small fragment removal system may improve extraction of renal calculi: an in vitro study.
Authors: Lazarus John , Wellmann Mark , Wulfsberg Jørgen , Ceccato Tommaso , John Jeff
Published: September 2024

Objective
To describe a novel Small Fragment Removal System (SFRS) which is hypothesized to improve stone fragment removal during flexible ureteroscopy in patients with urolithiasis.
The SFRS consists of three parts: a Syphon Ureteric Access Sheath (SUAS), a Dual Action Pump (DAP) and an Agitator.
This bench assessment aims to assess the SFRS’s impact on intra-renal pressure (IRP), irrigant flow rate and stone fragment removal compared to a traditional UAS.
Materials and Methods
A validated phantom kidney and fibre optic pressure sensor was used to assess IRP. Standardized irrigation via a flexible ureterorenoscope was instilled through a traditional UAS and compared to the novel SFRS. Both were 11/13Fr in size. Measured minute volume, IRP and percentage of stone fragments removed were compared.
Results
The mean IRP using a traditional UAS and SFRS was 24,3mmHg and 9,4mmHg respectively. The flow rate of the traditional UAS was 25mL/min, compared to 31mL/min with the SFRS attached.
During bolus fluid administration using the traditional UAS the maximum IRP increased to 41mmHg, compared to 9,3mmHg with the SFRS attached.
A mean of 42,7% (0,103g of 0,305g) of weighed dry stones were removed with the traditional UAS compared to 77,6% (0,233g of 0,299g) with the SFRS attached, p=0,017.
Conclusions
The described Small Fragment Removal System (SFRS) is different from traditional UASs by incorporating a syphoning mechanism. In addition, it has a Dual Action Pump which both boluses and augments the aspiration of irrigant by the Syphon. It further includes a deflectable Agitator to flush out stone fragments.
Alterations in Nailfold Videocapillaroscopy Among Patients with Connective Tissue Diseases Combined with Pulmonary Arterial Hypertension
Authors: Tang Zhicheng , Yang Fan , Wu Haolin , Zhao Ying , Shen Jingyi , Hong Huiming , Yin Fanzhang , Ma Xiaolei , Geng Linyu , Xu Xue , Wei Yu , Zhang Huayong
Published: September 2024

Objectives: This study investigates the correlation between abnormalities detected in nailfold videocapillaroscopy and pulmonary hypertension in patients with connective tissue disease (CTD). Additionally, it explores the potential diagnostic and predictive value of nailfold videocapillaroscopy in assessing microcirculation alterations in these patients.
Methods: A cross-sectional study involving 351 CTD patients and 30 healthy controls underwent nailfold videocapillaroscopy. Two independent physicians assessed the results qualitatively, semi-quantitatively, and quantitatively. Clinical characteristics and laboratory parameters were also recorded, with a focus on differences between CTD patients with pulmonary arterial hypertension (CTD-PAH) and those without (CTD-non-PAH).
Results: Among the patients, 16.5% (n=58) had pulmonary hypertension. CTD-PAH patients showed higher nailfold videocapillaroscopy scores (5.73±3.54 vs. 4.30±2.98, P=0.001) and larger capillary diameters (17.06±8.22 vs. 14.41±9.25, P=0.044) compared to CTD-non-PAH patients. Factors significantly influencing the nailfold videocapillaroscopy score included Raynaud's phenomenon, pulmonary hypertension, and the presence of anti-Scl-70 antibody. The ROC analysis yielded an AUC of 0.621 for predicting PAH. Additionally, pulmonary artery systolic pressure in CTD-PAH patients was positively correlated with both nailfold videocapillaroscopy score (R=0.618, B=3.26, P<0.001) and capillary diameter (R=0.541, B=1.23, P<0.001).
Conclusion: Nailfold videocapillaroscopy is a valuable tool in assessing CTD patients with pulmonary hypertension, providing a novel non-invasive method for evaluating this condition.
Cellular indexing of transcriptomes and epitopes (CITE-Seq) in hidradenitis suppurativa identifies dysregulated cell types in peripheral blood and facilitates diagnosis via machine learning
Authors: Liao Wilson , Kumar Sugandh , Orcales Faye , Shih Bobby , Fang Xiaohui , Yin Congcong , Yates Ashley , Dimitrion Peter , Neuhaus Isaac , Johnson Chandler , Adrianto Indra , Wiala Antonia , Hamzavi Iltefat H. , Zhou Li , Naik Haley , Posch Christian , Mi Qing-Sheng
Published: September 2024

Hidradenitis suppurativa (HS) is a chronic inflammatory skin condition characterized by painful nodules, abscesses, and scarring, predominantly affecting intertriginous regions. This study aimed to utilize single cell RNA and cell-surface protein sequencing (CITE-Seq) to delineate the immune composition of circulating cells in Hidradenitis suppurativa (HS) peripheral blood compared to healthy controls. CITE-Seq was used to analyze the gene and protein expression profiles of peripheral blood mononuclear cells (PBMCs) from 9 HS and 29 healthy controls. The study identified significant differences cell composition between HS patients and healthy controls, including increased proportions of CD14+ and CD16+ monocytes, cDC2, plasmablasts, and proliferating CD4+ T cells in HS patients. Differential expression analysis revealed upregulation of inflammatory markers such as TNF, IL1B, and NF-κB in monocytes, as well as chemokines and cell adhesion molecules involved in immune cell recruitment and tissue infiltration. Pathway enrichment analysis highlighted the involvement of IL-17, IL-26 and TNF signaling pathways in HS pathogenesis. Machine learning identified key markers for diagnostics and therapeutic development. The findings also support the potential for machine learning models to aid in the diagnosis of HS based on immune cell markers. These insights may inform future therapeutic strategies targeting specific immune pathways in HS.
Prognostic Impact of Renal Microcirculatory Dysfunction in Heart Failure Assessed by the Advanced Doppler technique, Superb Microvascular Imaging
Authors: Kayama Kiyomi , Kikuchi Shohei , Sugimoto Tadafumi , Seo Yoshihiro
Published: September 2024

The critical role of cardio-renal interactions in heart failure (HF) prognosis has gained increasing recognition, yet standardized methods for their assessment remain elusive. This study introduces a novel approach utilizing Superb Microvascular Imaging (SMI), an advanced ultrasound technique enabling detailed microvascular flow visualization, to evaluate renal microcirculation. We conducted a retrospective analysis of 78 patients who underwent renal ultrasonography with SMI between October 2020 and May 2023. Temporal changes in the Vascular Index (VI), which quantifies the blood flow signal area within the region of interest on SMI images, were measured. Key parameters included Maximum VI (Max.VI), Minimum VI (Min.VI), and the cyclic variation of VI, calculated as the intrarenal perfusion index (IRPI) = (Max.VI - Min.VI) / Max.VI within one cardiac cycle. The primary endpoint was a composite event (CE), defined as all-cause mortality or unplanned hospitalization due to worsening HF. Over a mean follow-up period of 1.6 ± 0.8 years, 13 of 78 patients (17%) experienced CEs. Patients with CEs exhibited significantly lower Max.VI and Min.VI values, while IRPI was significantly elevated in this group compared to those without CEs. Univariable Cox regression analyses revealed significant associations between Max.VI, Min.VI, and IRPI with CEs. In multivariable Cox regression analyses, Max.VI and Min.VI maintained significant associations with CEs after adjusting for creatinine, estimated central venous pressure, and intra-renal venous flow pattern. Kaplan-Meier analysis demonstrated that Max.VI (< 0.31, as determined by ROC analysis; 43% vs. 7%, log-rank p < 0.001), Min.VI (< 0.08, 42% vs. 8%, log-rank p < 0.001), and IRPI (> 0.70, 39% vs. 10%, log-rank p = 0.002) could effectively stratify CE prognosis. This novel application of SMI for renal circulation assessment provides valuable insights into HF prognosis and enables risk stratification beyond conventional markers.