bluuebunny/crossref_metadata_2025 huggingface dataset:
Effects of Elevated CO2 on the Fitness of Three Successive Generations of Lipaphis erysimi
Authors: Li Xue-Mei , Zhao Mu-Hua , Huang Feng , Shang Fang-Ge , Zhang Yun-Hui , Liu Cheng-Min , He Shuai-Jie , Wu Gang
Published: March 2022

To assess the effect of elevated CO2 on the development, fecundity, and population dynamic parameters of L. erysimi, the age-stage, two-sex life table was used to predict the individual fitness and population parameters of three successive generations of L. erysimi in this study. The results show that a significantly longer total pre-adult stage before oviposition (TPOP) was observed in the third generation compared with the first generation of L. erysimi under the 800 μL/L CO2 treatment. The fecundity is significantly lower in the 800 μL/L CO2 treatment than that in the 400 μL/L CO2 treatment in the third generation of L. erysimi, which indicates that elevated CO2 had a negative effect on the individual fitness parameters of L. erysimi. Additionally, the life expectancy (exj) is significantly lower under the 800 μL/L CO2 treatment than that under the 400 μL/L CO2 treatment in the three successive generations. A significantly higher intrinsic rate of increase (r) and finite rate of increase (λ) were found in the second generation compared with those in the first and third generations of L. erysimi under the 800 μL/L CO2 treatment. Moreover, significantly lower r and λ were observed under the 800 μL/L CO2 treatment compared with those under the 400 μL/L and 600 μL/L CO2 treatments in the first generation of L. erysimi, which indicates that elevated CO2 has a short-term effect on the population parameters (r and λ) of L. erysimi. Our experiment can provide the data for the comprehensive prevention and control of L. erysimi in the future with increasing CO2 levels.
The Effect of Diet on Colony Recognition and Cuticular Hydrocarbon Profiles of the Invasive Argentine Ant, Linepithema humile
Authors: van Wilgenburg Ellen , Mariotta Mario , Tsutsui Neil
Published: March 2022

Ants are some of the most abundant and ecologically successful terrestrial organisms, and invasive ants rank among the most damaging invasive species. The Argentine ant is a particularly well-studied invader, in part, because of the extreme social structure, known as unicoloniality, that occurs in introduced populations. Unicoloniality is characterized by the formation of geographically vast supercolonies, within which territorial behavior and intraspecific aggression are absent. Although there is considerable evidence supporting a genetic basis for the odor cues involved in colony recognition, some studies have suggested that diet may also influence colony recognition cues and, thus, colony structure. Here, we test the role for insect-derived recognition cues by performing a diet supplementation experiment in a natural field setting, and a more extreme dietary manipulation experiment in the lab. After one month, in both the field and the lab, we found that aggressive supercolonies remained aggressive toward each other and non-aggressive nests (from the same supercolony) remained non-aggressive, regardless of dietary treatment. In one lab treatment, we did observe a significant decrease in the level of aggression between different supercolonies that were fed the same diet, but aggression was still frequent. We did not see any evidence for cuticular hydrocarbon odor cues being transferred from prey to ants in any of the field treatments. In the more extreme lab treatment, however, several cuticular hydrocarbons were acquired from both roach and cricket insect prey (but not Drosophila). Based on these data, we conclude that dietary changes are unlikely to underlie changes in behavior or colony structure in Argentine ants in real-world settings. However, these results indicate that caution is warranted when interpreting the behaviors of animals that have been reared on diets that are substantially different from natural populations.
The Assessment of Endovascular Therapies in Ischemic Stroke: Management, Problems and Future Approaches
Authors: Popiela Tadeusz J. , Krzyściak Wirginia , Pilato Fabio , Ligęzka Anna , Bystrowska Beata , Bukowska-Strakova Karolina , Brzegowy Paweł , Muthusamy Karthik , Kozicz Tamas
Published: March 2022

Ischemic stroke accounts for over 80% of all strokes and is one of the leading causes of mortality and permanent disability worldwide. Intravenous administration of recombinant tissue plasminogen activator (rt-PA) is an approved treatment strategy for acute ischemic stroke of large arteries within 4.5 h of onset, and mechanical thrombectomy can be used for large arteries occlusion up to 24 h after onset. Improving diagnostic work up for acute treatment, reducing onset-to-needle time and urgent radiological access angiographic CT images (angioCT) and Magnetic Resonance Imaging (MRI) are real problems for many healthcare systems, which limits the number of patients with good prognosis in real world compared to the results of randomized controlled trials. The applied endovascular procedures demonstrated high efficacy, but some cellular mechanisms, following reperfusion, are still unknown. Changes in the morphology and function of mitochondria associated with reperfusion and ischemia-reperfusion neuronal death are still understudied research fields. Moreover, future research is needed to elucidate the relationship between continuously refined imaging techniques and the variable structure or physical properties of the clot along with vascular permeability and the pleiotropism of ischemic reperfusion lesions in the penumbra, in order to define targeted preventive procedures promoting long-term health benefits.
Facilitating Role of the 3D Viewing System in Tilted Microscope Positions for Cataract Surgery in Patients Unable to Lie Flat
Authors: Sandali Otman , Tahiri Joutei Hassani Rachid , Armia Balamoun Ashraf , El Sanharawi Mohamed , Borderie Vincent
Published: March 2022

Purpose: To assess the utility of the 3D viewing system in tilted microscope positions for the performance of cataract surgery in challenging positions, for patients with difficulty remaining supine. Methods: Prospective, single-center, single-surgeon, consecutive case series of patients undergoing surgery in an inclined position. Results: 21 eyes of 15 patients who had undergone surgery at inclined positions at angles of 20° to 80°, with a mean angle of 47.62°. Surgeon comfort was considered to be globally good. The surgeon rated red reflex perception and the impression of depth as good and stable in all cases. The operating time was slightly longer for patients inclined at angles of more than 50°. On the first day after surgery, BSCVA was 20/25 or better in all cases. No ocular complications occurred in any of the interventions. Conclusions: Due to the ocular-free design of the 3D system, the surgical procedure and the positioning of the surgeon remained almost identical to that for patients undergoing surgery in a supine position, maintaining the safety of the standard surgical approach.
Maternal, Perinatal and Neonatal Outcomes of Triplet Pregnancies According to Chorionicity: A Systematic Review of the Literature and Meta-Analysis
Authors: Bernal Claverol Mireia , Ruiz Minaya María , Aracil Moreno Irene , Tizón Santiago García , Pintado Recarte Pilar , Alvarez-Mon Melchor , Arribas Coral Bravo , Ortega Miguel A. , De Leon-Luis Juan A.
Published: March 2022

Triplet pregnancies are rare events that affect approximately 93 in 100,000 deliveries in the world, especially due to the increased use of assisted reproductive techniques and older maternal age. Triplet pregnancies are associated with a higher risk of fetal and maternal morbidity and mortality compared to twins and singletons. Chorionicity has been proposed as a major determinant of perinatal and maternal outcomes in triplet pregnancies, although further evidence is needed to clarify the extent and real influence of this factor. Thus, the aim of this study was to conduct a systematic review of the literature and a meta-analysis of the maternal and perinatal outcomes of triplet pregnancies, evaluating how chorionicity may influence these results. A total of 46 studies with 43,653 triplet pregnancies and 128,145 live births were included. Among the main results of our study, we found a broad spectrum of fetal and maternal complications, especially in the group of monochorionic and dichorionic pregnancies. Risk of admission to NICU, respiratory distress, sepsis, necrotizing enterocolitis, perinatal and intrauterine mortality were all found to be higher in non-TCTA pregnancies than in TCTA pregnancies. To date, our meta-analysis includes the largest population sample and number of studies conducted in this field, evaluating a wide variety of outcome measures. The heterogeneity and retrospective design of the studies included in our research represent the main limitations of this review. More evidence is needed to fully assess outcome measures that could not be studied in this review due to scarcity of publications or insufficient sample size.
Characteristic Immune Dynamics in COVID-19 Patients with Cardiac Dysfunction
Authors: Gonzalez Filipe André , Ângelo-Dias Miguel , Martins Catarina , Gomes Rui , Bacariza Jacobo , Fernandes Antero , Borrego Luís Miguel ,   EchoCrit Group
Published: March 2022

Background: We aimed to explore immune parameters in COVID-19 patients admitted to the intensive care unit (ICU) to identify distinctive features in patients with cardiac injury. Methods: A total of 30 COVID-19 patients >18 years admitted to the ICU were studied on days D1, D3 and D7 after admission. Cardiac function was assessed using speckle-tracking echocardiography (STE). Peripheral blood immunophenotyping, cardiac (pro-BNP; troponin) and inflammatory biomarkers were simultaneously evaluated. Results: Cardiac dysfunction (DYS) was detected by STE in 73% of patients: 40% left ventricle (LV) systolic dysfunction, 60% LV diastolic dysfunction, 37% right ventricle systolic dysfunction. High-sensitivity cardiac troponin (hs-cTn) was detectable in 43.3% of the patients with a median value of 13.00 ng/L. There were no significant differences between DYS and nDYS patients regarding mortality, organ dysfunction, cardiac (including hs-cTn) or inflammatory biomarkers. Patients with DYS showed persistently lower lymphocyte counts (median 896 [661–1837] cells/µL vs. 2141 [924–3306] cells/µL, p = 0.058), activated CD3 (median 85 [66–170] cells/µL vs. 186 [142–259] cells/µL, p = 0.047) and CD4 T cells (median 33 [28–40] cells/µL vs. 63 [48–79] cells/µL, p = 0.005), and higher effector memory T cells (TEM) at baseline (CD4%: 10.9 [6.4–19.2] vs. 5.9 [4.2–12.8], p = 0.025; CD8%: 15.7 [7.9–22.8] vs. 8.1 [7.7–13.7], p = 0.035; CD8 counts: 40 cells/µL [17–61] vs. 10 cells/µL [7–17], p = 0.011) than patients without cardiac dysfunction. Conclusion: Our study suggests an association between the immunological trait and cardiac dysfunction in severe COVID-19 patients.
In Silico Finite Element Modeling of Stress Distribution in Osteosynthesis after Pertrochanteric Fractures
Authors: Lorkowski Jacek , Pokorski Mieczyslaw
Published: March 2022

A stabilization method of pertrochanteric femur fractures is a contentious issue. Here, we assess the feasibility of rapid in silico 2D finite element modeling (FEM) to predict the distribution of stresses arising during the two most often used stabilization methods: gamma nail fixation (GNF) and dynamic hip screw (DHS). The modeling was based on standard pre-surgery radiographs of hip joints of 15 patients with pertrochanteric fractures of type A1, A2, and A3 according to the AO/OTA classification. The FEM showed that the stresses were similar for both GNF and DHS, with the medians ranging between 53–60 MPa and consistently lower for A1 than A3 fractures. Stresses also appeared in the fixation materials being about two-fold higher for GNF. Given similar bone stresses caused by both GNF and DHS but shorter surgery time, less extensive dissection, and faster patient mobilization, we submit that the GNF stabilization appears to be the most optimal system for pertrochanteric fractures. In silico FEM appears a viable perioperative method that helps predict the distribution of compressive stresses after osteosynthesis of pertrochanteric fractures. The promptness of modeling fits well into the rigid time framework of hip fracture surgery and may help optimize the fixation procedure for the best outcome. The study extends the use of FEM in complex orthopedic management. However, further datasets are required to firmly position the FEM in the treatment of pertrochanteric fractures.
Live Birth Rate in Women with Recurrent Pregnancy Loss after In Vitro Fertilization with Concomitant Intravenous Immunoglobulin and Prednisone
Authors: Egerup Pia , Nielsen Henriette Svarre , Andersen Anders Nyboe , Christiansen Ole Bjarne
Published: March 2022

Pregnancy loss after in vitro fertilization (IVF) is at least as common as after spontaneous conception. Recurrent pregnancy loss (RPL) may often have an immunological background, and it is therefore relevant to test immune-based interventions in these patients. The objective was to investigate the effect of immunotherapy with intravenous immunoglobulin (IvIg) and prednisone (PRS) as concomitant therapy to IVF in women with RPL after earlier IVF treatments. In a cohort study conducted at The Danish RPL Clinic, 41 women with three or more consecutive pregnancy losses after IVF underwent at least one further IVF cycle with concomitant immunotherapy from 2012 to 2017. The immunotherapy with IvIg and PRS was given before embryo transfer and repeatedly in the first trimester when pregnancy was achieved. Fourteen women (34.2%) achieved a live birth after the first embryo transfer with immunotherapy, and a total of 32/41 (78%) achieved a live birth after up to 4 embryo transfers. Baseline characteristics and the presence of autoantibodies were not significantly different among women achieving live birth or not. The observed 34% birth rate in women with RPL after IVF receiving immunotherapy appears higher than the expected 16–19% birth rate without immunotherapy and is similar to findings in a previous cohort from our clinic. Concomitant immunotherapy as described may be a promising intervention for women with RPL after IVF; however, the effect must be tested in a randomized controlled trial.
A Multimarker Model for Aberrant Cardiac Geometry after Preeclampsia
Authors: Mohseni-Alsalhi Zenab , Laven Sophie A. J. S. , Janssen Emma B. N. J. , Wagenaar Anique L. , van Kuijk Sander M. J. , Spaanderman Marc E. A. , Ghossein-Doha Chahinda
Published: March 2022

One out of four women with a history of preeclampsia shows abnormal cardiac remodeling consistent with subclinical heart failure (HF) in the first decade postpartum. Since these women are susceptible for developing remote symptomatic HF, development of a model for aberrant cardiac geometry as a first screening tool after delivery, is urgently needed. In this cross-sectional study, 752 preeclamptic women were included. Cardiovascular evaluation was conducted between six months and five years postpartum including cardiac ultrasound, systolic and diastolic blood pressure (SBP and DBP), plasma volume (PV) and biomarker assessment. We developed a multimarker model using uni- and multivariable linear regression and used the regression coefficients (RC) to develop a formula and estimate the aberrant cardiac remodeling in our population. Both SBP and PV were shown to be independently correlated with relative wall thickness (RWT) and left ventricular mass index (LVMi). C-reactive protein (CRP) and uric acid were independently correlated with RWT. Fibrinogen did not relate to either LVMi or RWT. This study displays markers of abnormal cardiac remodeling in former preeclamptic women, suggesting a combination of mechanical and biochemical factors that should be involved in worrisome chamber remodeling before clinical symptoms arise.
The Impact of Novel Anti-Diabetic Medications on CV Outcomes: A New Therapeutic Horizon for Diabetic and Non-Diabetic Cardiac Patients
Authors: Mazin Israel , Chernomordik Fernando , Fefer Paul , Matetzky Shlomi , Beigel Roy
Published: March 2022

It is estimated that in the past two decades the number of patients diagnosed with diabetes mellites (DM) has doubled. Despite significant progress in the treatment of cardiovascular disease (CVD), including novel anti-platelet agents, effective lipid-lowering medications, and advanced revascularization techniques, patients with DM still are least twice as likely to die of cardiovascular causes compared with their non-diabetic counterparts, and current guidelines define patients with DM at the highest risk for atherosclerotic cardiovascular disease and major adverse cardiovascular events (MACE). Over the last few years, there has been a breakthrough in anti-diabetic therapeutics, as two novel anti-diabetic classes have demonstrated cardiovascular benefit with consistently reduced MACE, and for some agents, also improvement in heart failure status as well as reduced cardiovascular and all-cause mortality. These include the sodium-glucose cotransporter-2 inhibitors and the glucagon-like peptide-1 receptor agonists. The benefits of these medications are thought to be derived not only from their anti-diabetic effect but also from additional mechanisms. The purpose of this review is to provide the everyday clinician a detailed review of the various agents within each class with regard to their specific characteristics and the effects on MACE and cardiovascular outcomes.
Mechanical Characteristics of Prestressed Concrete Curved Transition Section of Composite Bucket Foundations for Offshore Wind Turbines
Authors: Zhang Puyang , Xu Yunlong , Xiao Jiandong , Le Conghuan , Ding Hongyan
Published: March 2022

The composite bucket foundation (CBF) consists of a concrete curved transition section, a concrete beam-slab system, and a suction caisson and is increasingly used as the foundation for offshore wind turbines. The curved transition section transmits the upper load from the tower to the foundation, and its force and transmission characteristics are related to the safety of the entire wind turbine structure. The arced transition section has the characteristics of complex geometry, load conditions, and large curvature. It is difficult to determine its bearing characteristics and force transmission system. In this paper, the boundary conditions and loading device of the CBF model test are designed, and three 1:20 arced transition section model specimens are made. The mechanical characteristic experiments of CBF are used to analyze the failure process, failure characteristics, and seismic performance of the structure. Results show that the cracking effect of the arced transition section after prestressing is obviously better than that of a reinforced concrete arced transition section structure. The arced transition section specimens equipped with prestressed tendons can increase the structural cracking load ratio by about 35% for reinforced concrete members. The energy dissipation capacity of the specimens has been significantly improved, and the material properties can be fully utilized. The failure mode of the arced transition section structure under horizontal reciprocating load shows the characteristics of bending and shear failure.
ANN- and FEA-Based Assessment Equation for a Corroded Pipeline with a Single Corrosion Defect
Authors: Lo Michael , Karuppanan Saravanan , Ovinis Mark
Published: March 2022

Most of the standards available for the assessment of the failure pressure of corroded pipelines are limited in their ability to assess complex loadings, and their estimations are conservative. To overcome this research gap, this study employed an artificial neural network (ANN) model trained with data obtained using the finite element method (FEM) to develop an assessment equation to predict the failure pressure of a corroded pipeline with a single corrosion defect. A finite element analysis (FEA) of medium-toughness pipelines (API 5L X65) subjected to combined loads of internal pressure and longitudinal compressive stress was carried out. The results from the FEA with various corrosion geometric parameters and loads were used as the training dataset for the ANN. After the ANN was trained, its performance was evaluated, and its weights and biases were obtained for the development of a corrosion assessment equation. The prediction from the newly developed equation has a good correlation value, R2 of 0.9998, with percentage errors ranging from −1.16% to 1.78%, when compared with the FEA results. When compared with the failure pressure estimates based on the Det Norske Veritas (DNV-RP-F101) guidelines, the standard was more conservative in its prediction than the assessment equation developed in this study.
Epigenetics in Precision Nutrition
Authors: Li Xiang , Qi Lu
Published: March 2022

Precision nutrition is an emerging area of nutrition research, with primary focus on the individual variability in response to dietary and lifestyle factors, which are mainly determined by an individual’s intrinsic variations, such as those in genome, epigenome, and gut microbiome. The current research on precision nutrition is heavily focused on genome and gut microbiome, while epigenome (DNA methylation, non-coding RNAs, and histone modification) is largely neglected. The epigenome acts as the interface between the human genome and environmental stressors, including diets and lifestyle. Increasing evidence has suggested that epigenetic modifications, particularly DNA methylation, may determine the individual variability in metabolic health and response to dietary and lifestyle factors and, therefore, hold great promise in discovering novel markers for precision nutrition and potential targets for precision interventions. This review summarized recent studies on DNA methylation with obesity, diabetes, and cardiovascular disease, with more emphasis put in the relations of DNA methylation with nutrition and diet/lifestyle interventions. We also briefly reviewed other epigenetic events, such as non-coding RNAs, in relation to human health and nutrition, and discussed the potential role of epigenetics in the precision nutrition research.
Clinical Characteristics of Patients with Pyogenic Vertebral Osteomyelitis and Concurrent Infections and Their Clinical Outcomes
Authors: Kim Donghyun , Kim Jihye , Kim Taehwan
Published: March 2022

Patients with pyogenic vertebral osteomyelitis (PVO) often develop concurrent infections, and a significant number of these patients show rapid deterioration in their medical condition, leading to mortality without PVO-related structural instability or neurological deficits. To improve clinical outcomes, we investigated the clinical presentation and treatment outcomes of patients with PVO and concurrent infections. This study included 695 patients with PVO, of which 175 (25%) had concurrent infections and 520 (75%) did not. The clinical characteristics of the two groups were compared, and multivariable analysis was performed to identify the association between concurrent infections and clinical outcomes. Patients with concurrent infections were older and had more comorbidities than those without. Moreover, there were significant intergroup differences in the anatomical involvement of PVO, and patients with concurrent infections had a higher number of regions involved more frequently than those without concurrent infections (15% vs. 6%). In contrast, patients with concurrent infections showed a lower degree of focal invasiveness, including a lower incidence of posterior abscess (47% vs. 59%; p = 0.008) and fewer neurological impairments according to the American Spinal Injury Association grade (p < 0.001) than those without concurrent infections. The causative organisms also differed significantly between the two groups, and patients with concurrent infections had a greater proportion of Gram-negative infections (31% vs. 16%, respectively) and a smaller proportion of methicillin-resistant S. aureus infections than those without concurrent infections (6% vs. 24%). Consequently, their clinical outcomes were significantly different, and patients with concurrent infections showed lower recurrence and higher mortality rates. We investigated the 1-year recurrence and mortality rates and their 95% confidence intervals according to the types of concurrent infections and their time of diagnosis and found variations in these parameters. Our results, based on a large number of patients, can be practically used as a reasonable reference to warn clinicians of the clinical risks of concurrent infections in patients with PVO and to help predict their clinical outcomes.
Personalized Immunotherapies for Type 1 Diabetes: Who, What, When, and How?
Authors: Deligne Claire , You Sylvaine , Mallone Roberto
Published: March 2022

Our understanding of the immunopathological features of type 1 diabetes (T1D) has greatly improved over the past two decades and has shed light on disease heterogeneity dictated by multiple immune, metabolic, and clinical parameters. This may explain the limited effects of immunotherapies tested so far to durably revert or prevent T1D, for which life-long insulin replacement remains the only therapeutic option. In the era of omics and precision medicine, offering personalized treatment could contribute to turning this tide. Here, we discuss how to structure the selection of the right patient at the right time for the right treatment. This individualized therapeutic approach involves enrolling patients at a defined disease stage depending on the target and mode of action of the selected drug, and better stratifying patients based on their T1D endotype, reflecting intrinsic disease aggressiveness and immune context. To this end, biomarker screening will be critical, not only to help stratify patients and disease stage, but also to select the best predicted responders ahead of treatment and at early time points during clinical trials. This strategy could contribute to increase therapeutic efficacy, notably through the selection of drugs with complementary effects, and to further develop precision multi-hit medicine.
Predicting Inflation—A Holistic Approach
Authors: Avdiu Kujtim , Unger Stephan
Published: March 2022

The quantity equation is a well-established, theoretic, long-run concept that has been criticized for a variety of reasons, i.e., that no precise statements about causality or dynamics between money growth and inflation can be inferred from its components. These shortcomings can be tackled by estimating inflation based upon a holistic approach and the performance of a ceteris paribus analysis for various levels of quantity and velocity of money, as well as GDP. By testing the validity of the quantity equation, it is possible to evaluate possible effects of elevated budget deficits, unprecedented expansions of the monetary base caused by global lockdowns, and a crash in global productivity, on inflation. The main findings of this paper suggest that the level of productivity is the main driver of inflation. The quantity and velocity of money only play a subordinate role in the determination of the inflation level. If inflation is holistically seen as a function of the quantity and velocity of money, as well as general economic productivity, the level of inflation can be very well explained by comparing the supply side with general economic productivity.
Dietary Changes and Their Influence in the Development of Kidney Disease
Authors: Simopoulos Artemis P.
Published: March 2022

Human beings evolved on a diet that was balanced in omega-6/omega-3 fatty acids, and was rich in fruits and vegetables with a ratio of animal to vegetable intake of 35:65. Such a diet is a base-producing anti-inflammatory diet and one in which our genes have been programmed to respond. Current Western diets are acid-producing diets and are also high in omega-6 fatty acids, leading to a proinflammatory state. From an evolutionary perspective a mild systemic metabolic alkalosis, resulting from chronic net-base loading, is the natural and optimal systemic acid–base homeostasis of humans. Western diets that lead to a metabolic acidosis increase susceptibility to kidney disease as has been shown from the proinflammatory biomarkers that produce a cytokine storm. Healthy dietary patterns, including the Dietary Approach to Stop Hypertension (DASH) Study, and Mediterranean diets, such as the diet of Crete, both of which have a healthy dietary pattern, are associated with a lower incidence of chronic kidney disease and may prevent or decrease albuminuria and improve the estimated glomerular filtration rate (eGFR).
Do a Learner’s Background Languages Change with Increasing Exposure to L3? Comparing the Multilingual Phonological Development of Adolescents and Adults
Authors: Nelson Christina
Published: March 2022

The present study longitudinally explores regressive phonological cross-linguistic influence (CLI) in seven adolescents (aged 12–13) and seven adults (aged 21–39) by examining voice-onset time (VOT) of /p,t,k/ in their first, second, and third language (L1, L2, and L3, respectively). All participants had the same language combination (L1 German, L2 English, L3 Polish) and were recorded completing a range of production tasks in all three languages four times over the course of the first year of L3 learning. The scope of previous research on phonological CLI is thus broadened in two ways: (1) by tracing the development of all languages upon the arrival of a new language in a multilingual’s system longitudinally, and (2) by investigating CLI patterns in two age groups when input and learning environment are comparable. Previous L2 age studies have mostly only made retrospective assumptions about (target) language development, so that longitudinal data, including the entire language repertoire of multilingual speakers, are needed to substantiate claims made in that regard. For the adolescent group, significant changes to both their L1 and L2 over time were found, while the adults’ background languages remained relatively stable on the group level. However, for both groups, much individual variation was uncovered.
The Role of 3D-Printed Custom-Made Vertebral Body Implants in the Treatment of Spinal Tumors: A Systematic Review
Authors: Costanzo Roberta , Ferini Gianluca , Brunasso Lara , Bonosi Lapo , Porzio Massimiliano , Benigno Umberto Emanuele , Musso Sofia , Gerardi Rosa Maria , Giammalva Giuseppe Roberto , Paolini Federica , Palmisciano Paolo , Umana Giuseppe Emmanuele , Sturiale Carmelo Lucio , Di Bonaventura Rina , Iacopino Domenico Gerardo , Maugeri Rosario
Published: March 2022

In spinal surgery, 3D prothesis represents a useful instrument for spinal reconstruction after the removal of spinal tumors that require an “en bloc” resection. This represents a complex and demanding procedure, aiming to restore spinal length, alignment and weight-bearing capacity and to provide immediate stability. Thus, in this systematic review the authors searched the literature to investigate and discuss the advantages and limitations of using 3D-printed custom-made vertebral bodies in the treatment of spinal tumors. A systematic literature review was conducted following the PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses) statement, with no limits in terms of date of publication. The collected studies were exported to Mendeley. The articles were selected according to the following inclusion criteria: availability of full articles, full articles in English, studies regarding the implant of 3D custom-made prothesis after total or partial vertebral resection, studies regarding patients with a histologically confirmed diagnosis of primary spinal tumor or solitary bone metastasis; studies evaluating the implant of 3d custom-made prothesis in the cervical, thoracic, and lumbar spine. Nineteen published studies were included in this literature review, and include a total of 87 patients, 49 males (56.3%) and 38 females (43.7%). The main tumoral location and primary tumor diagnosis were evaluated. The 3D custom-made prothesis represents a feasible tool after tumor en-bloc resection in spinal reconstruction. This procedure is still evolving, and long-term follow-ups are mandatory to assess its safeness and usefulness.
Human Milk Macronutrients and Bioactive Molecules and Development of Regional Fat Depots in Western Australian Infants during the First 12 Months of Lactation
Authors: Gridneva Zoya , Rea Alethea , Lai Ching Tat , Tie Wan Jun , Kugananthan Sambavi , Warden Ashleigh H. , Perrella Sharon L. , Murray Kevin , Geddes Donna T.
Published: March 2022

We investigated associations between intakes of human milk (HM) components (macronutrients and biologically active molecules) and regional fat depots development in healthy term infants (n = 20) across the first year of lactation. Infant limb (mid-arm and mid-thigh) lean and fat areas were assessed by ultrasound imaging at 2, 5, 9 and 12 months of age. Concentrations of HM total protein, whey protein, casein, adiponectin, leptin, lysozyme, lactoferrin, secretory IGA, total carbohydrates, lactose, HM oligosaccharides (total HMO, calculated) and infant 24-h milk intake were measured, and infant calculated daily intakes (CDI) of HM components were determined. This pilot study shows higher 24-h milk intake was associated with a larger mid-arm fat area (p = 0.024), higher breastfeeding frequency was associated with larger mid-arm (p = 0.008) and mid-thigh (p < 0.001) fat areas. Lysozyme (p = 0.001) and HMO CDI (p = 0.004) were time-dependently associated with the mid-arm fat area. Intakes of HM components and breastfeeding parameters may modulate infant limb fat depots development during the first year of age and potentially promote favorable developmental programming of infant body composition; however, further studies are needed to confirm these findings.
Understanding the Complexities and Changes of the Astronaut Microbiome for Successful Long-Duration Space Missions
Authors: Tesei Donatella , Jewczynko Anna , Lynch Anne , Urbaniak Camilla
Published: March 2022

During space missions, astronauts are faced with a variety of challenges that are unique to spaceflight and that have been known to cause physiological changes in humans over a period of time. Several of these changes occur at the microbiome level, a complex ensemble of microbial communities residing in various anatomic sites of the human body, with a pivotal role in regulating the health and behavior of the host. The microbiome is essential for day-to-day physiological activities, and alterations in microbiome composition and function have been linked to various human diseases. For these reasons, understanding the impact of spaceflight and space conditions on the microbiome of astronauts is important to assess significant health risks that can emerge during long-term missions and to develop countermeasures. Here, we review various conditions that are caused by long-term space exploration and discuss the role of the microbiome in promoting or ameliorating these conditions, as well as space-related factors that impact microbiome composition. The topics explored pertain to microgravity, radiation, immunity, bone health, cognitive function, gender differences and pharmacomicrobiomics. Connections are made between the trifecta of spaceflight, the host and the microbiome, and the significance of these interactions for successful long-term space missions.
Synergy Effect and Symmetry-Induced Enhancement Effect of Surface Multi-Defects on Nanohardness by Quasi-Continuum Method
Authors: Zhang Zhongli , Wang Can , Ni Yushan
Published: March 2022

The quasicontinuum method has been applied to probe the thin film with surface multi-defects, which is commonly seen in nanoimprint technique and bulk micromachining. Three unilaterally distributed multi-defect models and six bilaterally distributed multi-defect models of Pt thin film have been carried out in nanoindentation. The results show that the nanohardness gradually decreases as the number of unilaterally distributed multi-defects increases, along with the increasingly low decline rate of the nanohardness. The synergy effect of the unilaterally distributed multi-defects has been highly evidenced by the critical load revision for dislocation emission of Pt thin film, and it is predicted into a universal form with the synergy coefficient among the existing multi-defects for FCC metals. Moreover, the nanohardness obviously increases when the bilaterally distributed multi-defects form into symmetrical couple, and it could be even greater than the one with defect-free surface, due to the symmetry-induced enhancement effect on nanohardness. The symmetry-induced enhancement coefficient has been brought out and has well explained the symmetry-induced enhancement effect of bilaterally distributed multi-defects on the nanohardness by a prediction formula. Furthermore, the characteristic length of symmetric relations has been brought out to calculate the symmetry-induced enhancement coefficient and it has been effectively predicted to equal to the sum of the adjacent distance between the surface defect and the indenter, the defect depth near the indenter, and the defect width for FCC metal.
Corrosion Resistance of Selective Laser Melted Ti6Al4V3Cu Alloy Produced Using Pre-Alloyed and Mixed Powder
Authors: Huang Gonghao , Fan Zefeng , Li Liu , Lu Yanjin , Lin Jinxin
Published: March 2022

Metallic elemental powder mixture and pre-alloyed metallic powder are both frequently used powder feedstock in the additive manufacturing process. However, little research has been conducted to compare the corrosion behavior of selective laser melting (SLM) alloys, fabricated by pre-alloyed metallic powder and mixed metallic powder. Hence, it is important to investigate the corrosion behavior of SLMed alloys, as well as the corresponding cast ingot, with the aim to better understand the feasibility of designing new materials. In this work, the SLM-produced Ti6Al4V3Cu alloys were manufactured using a metallic elemental powder mixture and pre-alloyed metallic powder, respectively. The corrosion behavior of the different Ti6Al4V3Cu alloys was investigated in following electrochemical tests and ion release measurements. The results showed that the Ti6Al4V3Cu alloy prepared by pre-alloyed metallic powder showed better corrosion resistance than that produced from mixed metallic powder. Moreover, the SLM-produced Ti6Al4V3Cu alloys performed significantly better in corrosion resistance than the cast Ti6Al4V3Cu. The results are expected to achieve a better understanding of the feasibility of designing new materials using mixed powders, contributing to reducing development costs and cycles.
Physical Properties of Eco-Sustainable Form-Stable Phase Change Materials Included in Mortars Suitable for Buildings Located in Different Continental Regions
Authors: Sarcinella Antonella , de Aguiar José Luís Barroso , Frigione Mariaenrica
Published: March 2022

Starting from two low-cost, low-environmental-impact polymers belonging to the Polyethylene Glycol (PEG) family, i.e., PEG 800 and PEG 1000, two form-stable phase change materials were produced. The two PEGs differ in molecular weight and, as a consequence, the melting and crystallization range of temperatures. The PCMs were obtained, including the PEG, in a liquid state, inside the pores of Lecce Stone flakes, obtained as waste pieces from its processing. A simple and inexpensive impregnation process was selected to produce the PCMs, thus adopting low-environmental-impact materials and cheap processes, and respecting circular economy principles. The two PCMs, the first composed of PEG 800, namely LS/PEG800, and the second composed of a 50/50%wt. mix of the different LS/PEGs, i.e., LS/PEG800_LS/PEG1000, were added as aggregates to four types of mortars, based on aerial and hydraulic lime, gypsum, and cement. The obtained mortars were characterized in their fresh state to assess their workability, and in a solid state after a proper cure to determine their characteristic Latent Heat Thermal Energy Storage (LHTES) properties and mechanical properties in both flexural and compressive modes, taking the mortars not containing any PCM as the reference. The results revealed that, with the proper selection of mortar formulations, it was possible to achieve suitable workability and adequate mechanical characteristics. The selection of a PEG with a low range of phase change temperatures, such as PEG 800, allows one to obtain mortars characterized by a melting/crystallization range that can be considered appropriate in applications characterized by cold climates. The production of a mixed PCM, composed of both PEGs, led to mortars displaying a large interval of melting/crystallization temperatures, which could be suitable in both warm and cold climates.
Turbostratic Carbon/Graphene Prepared via the Dry Ice in Flames Method and Its Purification Using Different Routes: A Comparative Study
Authors: Cuadros-Lugo Eduardo , Piñon-Espitia Manuel , Martinez-Rodríguez Harby A. , Lardizabal-Gutierrez Daniel , Estrada-Guel Ivanovich , Herrera-Ramirez Jose M. , Carreño-Gallardo Caleb
Published: March 2022

Although the dry ice method used to synthesize turbostratic carbon/graphene is little known and used, it has significant advantages over others, such as the following: it is low cost, simple, and a large quantity of material can be obtained using some inorganic and highly available acids (which can be reused). Despite the above advantages, the main reason for its incipient development is the resulting presence of magnesium oxide in the final product. In the present work, three different treatments were tested to remove this remnant using some acid chemical leaching processes, including hydrochloric acid, aqua regia, and piranha solution. Based on the experimental evidence, it was determined that using aqua regia and combining the leaching process with mechanical milling was the most efficient way of removing such a remnant, the residue being only 0.9 wt.%. This value is low compared to that obtained with the other acid leaching solutions and purification processes (2.8–29.6 wt.%). A mandatory high-energy mechanical milling stage was necessary during this treatment to expose and dissolve the highly insoluble oxide without secondary chemical reactions on the turbostratic carbon. High-energy mechanical milling is an effective route to exfoliate graphite, which allows the magnesium oxide to be more susceptible to acid treatment. A yield of turbostratic carbon/graphene of 1 wt.% was obtained from the metallic Mg. The obtained surface area was 504.8 m2g−1; this high value resulting from the intense exfoliation can potentiate the use of this material for a wide variety of applications.
Fucoxanthin Loaded in Palm Stearin- and Cholesterol-Based Solid Lipid Nanoparticle-Microcapsules, with Improved Stability and Bioavailability In Vivo
Authors: Chen Yaxin , He Niaoniao , Yang Ting , Cai Shuyun , Zhang Yi , Lin Jinjing , Huang Mingqing , Chen Weizhu , Zhang Yiping , Hong Zhuan
Published: March 2022

Fucoxanthin (FX) is a marine carotenoid that has proven to be a promising marine drug due to the multiple bioactivities it possesses. However, the instability and poor bioavailability of FX greatly limit its application in pharmaceuticals or functional foods. In this study, the creative construction of a solid lipid nanoparticle-microcapsule delivery system using mixed lipids of palm stearin and cholesterol wrapped with gelatin/Arabic gum to load lipophilic FX was fabricated, aiming to improve the stability and bioavailability of FX. The results showed that the encapsulated efficiency (EE) and drug loading capacity (LC) of optimized FX microcapsules (FX-MCs) obtained were as high as 96.24 ± 4.60% and 0.85 ± 0.04%, respectively, after single-factor experiments. The average particle size was 1154 ± 54 nm with negative Zeta potential (−20.71 ± 0.93 mV) as depicted with size-zeta potential spectrometer. The differential scanning calorimeter (DSC) and thermogravimetric analyzer (TG) results indicated that FX-MC has a higher Tg and slower weight loss than FX monomers (FX crystal) and blank MCs. Besides, The Fourier transform infrared spectrometer (FTIR) confirmed the good double encapsulation of FX into the solid lipid and composite coacervate. Moreover, the encapsulated FX showed higher storage stability, sustained release (55.02 ± 2.80% release in 8 h), and significantly improved bioavailability (712.33%) when compared to free FX. The research results can provide a principle theoretical basis for the development and application of FX in pharmaceuticals or functional foods.
Opioid-Sparing Analgesia Impacts the Perioperative Anesthetic Management in Major Abdominal Surgery
Authors: Jipa Miruna , Isac Sebastian , Klimko Artsiom , Simion-Cotorogea Mihail , Martac Cristina , Cobilinschi Cristian , Droc Gabriela
Published: March 2022

Background and Objectives: The management of acute postoperative pain (APP) following major abdominal surgery implies various analgetic strategies. Opioids lie at the core of every analgesia protocol, despite their side effect profile. To limit patients’ exposure to opioids, considerable effort has been made to define new opioid-sparing anesthesia techniques relying on multimodal analgesia. Our study aims to investigate the role of adjuvant multimodal analgesic agents, such as ketamine, lidocaine, and epidural analgesia in perioperative pain control, the incidence of postoperative cognitive dysfunction (POCD), and the incidence of postoperative nausea and vomiting (PONV) after major abdominal surgery. Materials and Methods: This is a clinical, observational, randomized, monocentric study, in which 80 patients were enrolled and divided into three groups: Standard group, C (n = 32), where patients received perioperative opioids combined with a fixed regimen of metamizole/acetaminophen for pain control; co-analgetic group, Co-A (n = 26), where, in addition to standard therapy, patients received perioperative systemic ketamine and lidocaine; and the epidural group, EA (n = 22), which included patients that received standard perioperative analgetic therapy combined with epidural analgesia. We considered the primary outcome, the postoperative pain intensity, assessed by the visual analogue scale (VAS) at 1 h, 6 h, and 12 h postoperatively. The secondary outcomes were the total intraoperative fentanyl dose, total postoperative morphine dose, maximal intraoperative sevoflurane concentration, confusion assessment method for intensive care units score (CAM-ICU) at 1 h, 6 h, and 12 h postoperatively, and the postoperative dose of ondansetron as a marker for postoperative nausea and vomiting (PONV) severity. Results: We observed a significant decrease in VAS score, as the primary outcome, for both multimodal analgesic regimens, as compared to the control. Moreover, the intraoperative fentanyl and postoperative morphine doses were, consequently, reduced. The maximal sevoflurane concentration and POCD were reduced by EA. No differences were observed between groups concerning PONV severity. Conclusions: Multimodal analgesia concepts should be individualized based on the patient’s needs and consent. Efforts should be made to develop strategies that can aid in the reduction of opioid use in a perioperative setting and improve the standard of care.