bluuebunny/crossref_metadata_2025 huggingface dataset:
Inhibition of adenylyl cyclase 1 by ST034307 inhibits IP3-evoked changes in sino-atrial node beat rate
Authors: Bose Samuel J. , Read Matthew J. , Akerman Emily , Capel Rebecca A. , Ayagama Thamali , Russell Angela , Terrar Derek A. , Zaccolo Manuela , Burton Rebecca A. B.
Published: August 2022

Atrial arrhythmias, such as atrial fibrillation (AF), are a major mortality risk and a leading cause of stroke. The IP3 signalling pathway has been proposed as an atrial-specific target for AF therapy, and atrial IP3 signalling has been linked to the activation of calcium sensitive adenylyl cyclases AC1 and AC8. We investigated the involvement of AC1 in the response of intact mouse atrial tissue and isolated guinea pig atrial and sino-atrial node (SAN) cells to the α-adrenoceptor agonist phenylephrine (PE) using the selective AC1 inhibitor ST034307. The maximum rate change of spontaneously beating mouse right atrial tissue exposed to PE was reduced from 14.5% to 8.2% (p = 0.005) in the presence of 1 μM ST034307, whereas the increase in tension generated in paced left atrial tissue in the presence of PE was not inhibited by ST034307 (Control = 14.2%, ST034307 = 16.3%; p > 0.05). Experiments were performed using isolated guinea pig atrial and SAN cells loaded with Fluo-5F-AM to record changes in calcium transients (CaT) generated by 10 μM PE in the presence and absence of 1 μM ST034307. ST034307 significantly reduced the beating rate of SAN cells (0.34-fold decrease; p = 0.003) but did not inhibit changes in CaT amplitude in response to PE in atrial cells. The results presented here demonstrate pharmacologically the involvement of AC1 in the downstream response of atrial pacemaker activity to α-adrenoreceptor stimulation and IP3R calcium release.
Recent advances on development of p21-activated kinase 4 inhibitors as anti-tumor agents
Authors: Li Yang , Lu Qing , Xie Chenghu , Yu Yiming , Zhang Ao
Published: August 2022

The p21-activated kinase 4 (PAK4) is a member of the PAKs family. It is overexpressed in multiple tumor tissues. Pharmacological inhibition of PAK4 attenuates proliferation, migration, and invasion of cancer cells. Recent studies revealed that inhibition of PAK4 sensitizes immunotherapy which has been extensively exploited as a new strategy to treat cancer. In the past few years, a large number of PAK4 inhibitors have been reported. Of note, the allosteric inhibitor KPT-9274 has been tested in phase Ⅰ clinic trials. Herein, we provide an update on recent research progress on the PAK4 mediated signaling pathway and highlight the development of the PAK4 small molecular inhibitors in recent 5 years. Meanwhile, challenges, limitations, and future developmental directions will be discussed as well.
Comprehensive elaboration of circular RNA in multiple myeloma
Authors: Zhu Chunsheng , Guo Aoxiang , Sun Bao , Zhou Zheng
Published: August 2022

Circular RNAs (circRNAs), a novel category of endogenous non-coding RNAs, are usually well conserved across different species with a covalent closed-loop structure. Existing and emerging evidence confirms that circRNAs can function as regulators of alternative splicing, microRNA and RNA-binding protein sponges and translation, as well as gene transcription. In consideration of their multi-faceted functions, circRNAs are critically involved in hematological malignancies including multiple myeloma (MM). In particular, circRNAs have been found to play vital roles in tumor microenvironment and drug resistance, which may grant them potential roles as biomarkers for MM diagnosis and targeted therapy. In this review, we comprehensively elaborate the current state-of-the-art knowledge of circRNAs in MM, and then focus on their potential as biomarkers in diagnosis and therapy of MM.
S-3′-hydroxy-7′, 2′, 4′-trimethoxyisoxane, a novel ferroptosis inducer, promotes NSCLC cell death through inhibiting Nrf2/HO-1 signaling pathway
Authors: Chen Jing , Zhou Songlin , Zhang Xian , Zhao Huange
Published: August 2022

Background: Ferroptosis is a newly discovered and promising non-apoptotic programmed cell death (PCD), and inducing ferroptosis in cancer cells could open up a novel avenue for drug screening and cancer therapy. S-3′-hydroxy-7′, 2′, 4′-trimethoxyisoxane (ShtIX), a new isoflavane compound, has been reported to possess cytotoxicity in non-small cell lung cancer (NSCLC). The aim of this research is to explore the ShtIX-induced cell death form and its underlying molecular mechanism in NSCLC cells.

Methods: Cell proliferation, cell cycle arrest, and cell death tests were used to assess the ability of ShtIX to kill NSCLC cells. Iron metabolism, Fe2+ content, reactive oxygen species (ROS) production, lipid peroxide (MDA) level, glutathione (GSH) level, and glutathione peroxidase 4 (GPX4) level were used to determine ferroptosis caused by ShtIX. We employed western blot, quantitative real-time PCR, and Nrf2 interference in NSCLC cells to investigate the roles of Nrf2/HO-1 in ShtIX-induced ferroptosis. In a xenograft nude mouse model, the anticancer efficacy of ShtIX and the function of ferroptosis were studied.

Results: Our research shows that ShtIX can selectively kill NSCLC cells while sparing normal cells and that ShtIX-induced cell death can be efficiently reversed by the ferroptosis inhibitors and the iron chelator, but not by other cell death inhibitors. After cells were treated with ShtIX, there was an increase in Fe2+ content and lipid peroxidation accumulation, as well as a drop in GSH and GPX4 levels, all of which are indicators of ferroptosis. ShtIX also reduced the expression of Nrf2 and HO-1, and genetic Nrf2 silencing in NSCLC enhanced the effect of ShtIX-induced ferroptosis. Additionally, ShtIX retards tumor growth and induced ferroptosis through Nrf2/HO-1 signal pathway in the A549 xenograft model, whereas Fer-1 lessens the anticancer effect.

Conclusion: This work provided the evidence that ShtIX caused ferroptosis in NSCLC cells, and inhibiting the Nrf2/HO-1 pathway can considerably exacerbate the effect of ShtIX-induced ferroptosis. The study establishes ShtIX as a promising natural ferroptosis inducer for the treatment of NSCLC.
Exploring potential antidiabetic and anti-inflammatory flavonoids from Euphorbia humifusa with an integrated strategy
Authors: Rakotondrabe Tojofaniry Fabien , Fan Minxia , Guo Mingquan
Published: August 2022

E. humifusa Willd, a monoecious annual plant, native to Eastern Asia, has been traditionally attributed to the treatment and prevention of miscellaneous diseases, including diabetes mellitus and its associated complications. Earlier studies have supported this species’ pharmacological efficacies including its antibacterial, antidiabetic, and anti-inflammatory properties. Even so, the underlying bioactive components with their mechanisms of action associated with its antidiabetic and anti-inflammatory effects remain elusive. The preamble in vitro assessments of the crude extract and its different fractions revealed that the n-butanol fraction (EHNB) exhibited the best activity, which was subsequently subjected to a rapid screening of candidate ligands through bio-affinity ultrafiltration with the two enzyme targets: α-glucosidase (α-Glu) and cycloxygenase-2 (COX-2) combined with UPLC/QTOF-MS. As a result, 7 compounds were identified from EHNB, among them, vitexin and astragalin were screened out as the most active ligand compounds. Vitexin showed great specific binding (SB) affinity values of 1.26 toward α-Glu and 1.32 toward COX-2, while astragalin showed 1.32 and 1.36, respectively. The docking simulation results exhibited strong interactions of vitexin and astragalin with the key residues of the enzyme targets, suggesting their possible mechanisms of action. The in vitro antidiabetic validation revealed noticeable half-maximal inhibitory effects (IC50) of 36.38 ± 3.06 µM for vitexin and 42.47 ± 4.13 µM for astragalin, much better than that of the positive drug acarbose (109.54 ± 14.23 µM). Similarly, these two compounds showed the inhibitory activity against COX-2 with the half-maximal inhibitory effects (IC50) at 27.91 ± 1.74 µM and 49.05 ± 1.49 µM, respectively. Therefore, these two flavonoid compounds (vitexin and astragalin) were speculated as potential antidiabetic and anti-inflammatory compounds from E. humifusa. Taken together, the integrated strategy applied to E. humifusa led to the fast identification of two potential double-acting flavonoids and enlightened its antidiabetic and anti-inflammatory uses. Besides these findings, the integrated strategy in this study could also be used to facilitate the rapid discovery and development of active candidates from other traditional herbal medicines against multi-drug targets and to aid in revealing their mechanisms of action for their traditional uses.
Nucleic acid strategies for infectious disease treatments: The nanoparticle-based oral delivery route
Authors: Chen Fengqian , Liu Qi , Xiong Yang , Xu Li
Published: August 2022

Therapies based on orally administrated nucleic acids have significant potential for the treatment of infectious diseases, including chronic inflammatory diseases such as inflammatory bowel disease (IBD)-associated with the gastrointestinal (GI) tract, and infectious and acute contagious diseases like coronavirus disease 2019 (COVID-19). This is because nucleic acids could precisely regulate susceptibility genes in regulating the pro- and anti-inflammatory cytokines expression related to the infections. Unfortunately, gene delivery remains a major hurdle due to multiple intracellular and extracellular barriers. This review thoroughly discusses the challenges of nanoparticle-based nucleic acid gene deliveries and strategies for overcoming delivery barriers to the inflammatory sites. Oral nucleic acid delivery case studies were also present as vital examples of applications in infectious diseases such as IBD and COVID-19.
Dipolarization-induced energetic electron precipitation during continuously active periods and isolated substorms
Authors: Shukhtina M. A. , Sergeev V. A. , Rogov D. D.
Published: August 2022

Recently we proposed a spatio-temporal model of auroral absorption for isolated substorms based on linear prediction filter technique, which describes the precipitation effects as a sum of properly weighted and time-delayed contributions of short dipolarizations/injections inferred from magnetic MPB index. Here we apply a similar approach to a more general and practically important type of continuous activity including substorms clusters, which is accompanied by intense energetic electron precipitation lasting for many hours and may affect ozone concentration and climate. Unexpectedly, in spite of very different geophysical background, the derived precipitation response to unit-scale injection appeared almost identical to that obtained for isolated substorms. Significant part of absorption variance during active non-storm periods turns out to be the result of superposition of previous injections with short memory less than 4 h. Our results indicate that, while the injection efficiency is roughly the same, large difference in precipitated fluxes and ionospheric response between two different types of activity is mostly provided by a more frequent appearance and increased intensities of dipolarizations/injections during active periods. In both event types dipolarizations are the decisive factor which determines energetic electron precipitation.
Effects of curvature radius on flexible eddy current array sensors for the curved surface of metal components
Authors: Deng Zhiyang , Li Danyu , Qi Pan , Shao Wenbin , Chen Tao , Song Xiaochun , Kang Yihua
Published: August 2022

The flexible eddy current array sensor owns the advantages of high sensitivity and strong adaptability, but the results are always affected by the curvature radius of complex curved surfaces. The relationship between the curvature radius of the curved surface and detection signals for surface-breaking cracks is mainly discussed. The change of magnetic field caused by the curved surface in the present eddy current testing is specially pointed out, which manifest themselves in the compression or enhancement of the testing signal in its peak value and the baseline drifts. Simulation and experimental results indicate that the concave surface weakens the signal, while the convex surface enhances the signal. The signal amplitude decreases with the decrease in the curvature radius for the concave surface, while it is the opposite for the convex surface. Meanwhile, coil spacing significantly affects the amplitude–curvature radius curve. Furthermore, the fluctuation characteristic affected by the curvature radius under different coil spacing is analyzed. The discovery and results will benefit the quantitative evaluation of flexible eddy current array testing.
A Comparison of MRI Quantitative Susceptibility Mapping and TRUST-Based Measures of Brain Venous Oxygen Saturation in Sickle Cell Anaemia
Authors: Murdoch Russell , Stotesbury Hanne , Hales Patrick W. , Kawadler Jamie M. , Kölbel Melanie , Clark Christopher A. , Kirkham Fenella J. , Shmueli Karin
Published: August 2022

In recent years, interest has grown in the potential for magnetic resonance imaging (MRI) measures of venous oxygen saturation (Yv) to improve neurological risk prediction. T2-relaxation-under-spin-tagging (TRUST) is an MRI technique which has revealed changes in Yv in patients with sickle cell anemia (SCA). However, prior studies comparing Yv in patients with SCA relative to healthy controls have reported opposing results depending on whether the calibration model, developed to convert blood T2 to Yv, is based on healthy human hemoglobin (HbA), bovine hemoglobin (HbBV) or sickle hemoglobin (HbS). MRI Quantitative Susceptibility Mapping (QSM) is an alternative technique that may hold promise for estimating Yv in SCA as blood magnetic susceptibility is linearly dependent upon Yv, and no significant difference has been found between the magnetic susceptibility of HbA and HbS. Therefore, the aim of this study was to compare estimates of Yv using QSM and TRUST with five published calibration models in healthy controls and patients with SCA. 17 patients with SCA and 13 healthy controls underwent MRI. Susceptibility maps were calculated from a multi-parametric mapping acquisition and Yv was calculated from the mean susceptibility in a region of interest in the superior sagittal sinus. TRUST estimates of T2, within a similar but much smaller region, were converted to Yv using five different calibration models. Correlation and Bland-Altman analyses were performed to compare estimates of Yv between TRUST and QSM methods. For each method, t-tests were also used to explore group-wise differences between patients with SCA and healthy controls. In healthy controls, significant correlations were observed between QSM and TRUST measures of Yv, while in SCA, there were no such correlations. The magnitude and direction of group-wise differences in Yv varied with method. The TRUST-HbBV and QSM methods suggested decreased Yv in SCA relative to healthy controls, while the TRUST-HbS (p < 0.01) and TRUST-HbA models suggested increased Yv in SCA as in previous studies. Further validation of all MRI measures of Yv, relative to ground truth measures such as O15 PET and jugular vein catheterization, is required in SCA before QSM or TRUST methods can be considered for neurological risk prediction.
Malat1 deficiency prevents hypoxia-induced lung dysfunction by protecting the access to alveoli
Authors: Sallé-Lefort Sandrine , Miard Stéphanie , Henry Cyndi , Arias-Reyes Christian , Marcouiller François , Beaulieu Marie-Josée , Aubin Sophie , Lechasseur Ariane , Jubinville Éric , Marsolais David , Morissette Mathieu C. , Joseph Vincent , Soliz Jorge , Bossé Ynuk , Picard Frédéric
Published: August 2022

Hypoxia is common in lung diseases and a potent stimulator of the long non-coding RNA Metastasis-Associated Lung Adenocarcinoma Transcript 1 (MALAT1). Herein, we investigated the impact of Malat1 on hypoxia-induced lung dysfunction in mice. Malat1-deficient mice and their wild-type littermates were tested after 8 days of normoxia or hypoxia (10% oxygen). Hypoxia decreased elastance of the lung by increasing lung volume and caused in vivo hyperresponsiveness to methacholine without altering the contraction of airway smooth muscle. Malat1 deficiency also modestly decreased lung elastance but only when tested at low lung volumes and without altering lung volume and airway smooth muscle contraction. The in vivo responsiveness to methacholine was also attenuated by Malat1 deficiency, at least when elastance, a readout sensitive to small airway closure, was used to assess the response. More impressively, in vivo hyperresponsiveness to methacholine caused by hypoxia was virtually absent in Malat1-deficient mice, especially when hysteresivity, a readout sensitive to small airway narrowing heterogeneity, was used to assess the response. Malat1 deficiency also increased the coefficient of oxygen extraction and decreased ventilation in conscious mice, suggesting improvements in gas exchange and in clinical signs of respiratory distress during natural breathing. Combined with a lower elastance at low lung volumes at baseline, as well as a decreased propensity for small airway closure and narrowing heterogeneity during a methacholine challenge, these findings represent compelling evidence suggesting that the lack of Malat1 protects the access to alveoli for air entering the lung.
The application of artificial gravity in medicine and space
Authors: Isasi Eugenia , Isasi Maria E. , van Loon Jack J. W. A.
Published: August 2022

Gravity plays a crucial role in physiology. The lack of gravity, like in long duration spaceflight missions, cause pathologies in e.g., the musculoskeletal system, cardiovascular deconditioning, immune system deprivation or brain abnormalities, to just mention a few. The application of artificial gravity through short-arm human centrifugation (SAHC) has been studied as a possible countermeasure to treat spaceflight deconditioning. However, hypergravity protocols applied by using SAHC have also been used to treat different, ground-based pathologies. Such gravitational therapies have been applied in Uruguay for more than four decades now. The aim of this overview is to summarize the most important findings about the effects of gravitational therapy in different, mainly vascular based pathologies according to the experience in the Gravitational Therapy Center and to discuss the current research in the field of hypergravity applications in medicine but also as multisystem countermeasure for near weightlessness pathologies. New insight is needed on the use of hypergravity in medicine and space research and application.
Knockdown of P2Y4 ameliorates sepsis-induced acute kidney injury in mice via inhibiting the activation of the NF-κB/MMP8 axis
Authors: Wang Maojuan , Jiang Fan , Zhang Lian , Zhang Juan , Xie Hong
Published: August 2022

Sepsis-induced acute kidney injury (S-AKI) has emerged as a frequent and life-threatening complication in critically ill patients, which is characterized by a systematic inflammatory response and a rapid decline in kidney function. P2Y4, a member of G protein–coupled P2Y nucleotide receptor family, has been reported to serve as a crucial player in inflammatory responses during the development of neurocognitive disorder and myocardial infarction. Nonetheless, the biological role of P2Y4 in S-AKI remains largely unclear. This study aimed to decipher the biological role of P2Y4 in S-AKI and illuminate the potential mechanisms. In this study, S-AKI models were successfully established in mice via cecal ligation and puncture. Results showed that the kidney tissues from S-AKI mouse models exhibited a higher P2Y4 expression level than from the sham-operated group. Knockdown of P2Y4 was found to remarkably alleviate kidney damage and reduce inflammatory response in mice of S-AKI models. Moreover, P2Y4 ablation inhibited the activation of the NF-κB/MMP-8 signaling axis. Additionally, mechanistic studies revealed that rescuing MMP-8 reversed the alleviating effects of P2Y4 knockdown against renal cell damage. Collectively, our findings indicate that P2Y4 knockdown ameliorated S-AKI in mice via inhibiting the activation of the NF-κB/MMP-8 axis and that P2Y4 may represent a novel therapeutic target for S-AKI patients.
Effects of chains squat training with different chain load ratio on the explosive strength of young basketball players’ lower limbs
Authors: Jiang Dongting , Xu Gang
Published: August 2022

The purpose of this study was to explore the effects of the chain squat training (CST) with different chain load ratio (0, 10%, 20% and 30%) on the explosive power of the lower limbs of adolescent male basketball players. Forty-four youth basketball players (age 15.48 ± 0.81 years, body mass 78.86 ± 12.04 kg, height 184.95 ± 6.71 cm) were randomly allocated to one of the four groups: traditional squat training (TST), 10% chains squat training (10% CST), 20% chains squat training (20% CST), and 30% chains squat training (30% CST). Training interventions were performed 2 times per week for 6 weeks, and at the week before (Pre) and after (Post) the 6-week CST program with different chain load ratio, the no-step vertical jump, standing long jump, 15 m shuttle run, 1 R M squat and 30 m sprint test were performed. A 4 (group) × 2 (time) repeated measures analysis of variances (ANOVA) was calculated to show the scatter of each variable, and the Bonferroni’s post-hoc test was used for multiple comparisons, in addition the partial eta-squared (η2) was calculated as an estimate of the ES. Significant time × group interaction was noticed for the no-step vertical jump (p< 0.001; η2= 0.611), standing long jump (p< 0.001; η2= 0.490) and 1 R M squat (p< 0.01; η2= 0.333) indicating that better improvements appear in CST compared to TST. However, significant time × group interaction was noted for 15 m shuttle run (p< 0.001; η2= 0.428), in favor of TST compared to CST. In addition, the improvements in 30 m sprint were similar between all groups. In conclusion, CST with more chain load has better training effects on lower limb explosive strength and maximum strength, based on the improvement in 1 R M squat and jumping performance. Besides, compared with TST, CST with more chain load might not help to develop better velocity adaptation at higher range of movement.
Study about photoinduction-based technology for trapping small brown planthopper
Authors: Zhan Shaoqi , Sheng Zhentao , Liu Yiming , Xu Ke , Wang Jiahui , Cao Weixing , Tian Yongchao , Ni Jun
Published: August 2022

To provide a theory to guide the selection of the illumination parameters of light emitting diode (LED)-based light sources used for trapping Laodelphax striatellus, we used LED light sources and devices built in-house to detect L. striatellus phototactic behavior. Through phototaxis screening experiments of different light sources and the comparative experimental method, we analyzed the response patterns of L. striatellus to wavelength, light intensity, layout, flash frequency of monochromatic light sources, as well as combined color light sources, and discussed the mechanisms of the phototactic behavior of L. striatellus under different light sources. The results of the monochromatic light experiment showed that the trapping rate of the L. striatellus to the linear blue light source of 460 nm was the highest and was also significantly affected by the light intensity. The results of the experiments with the combined color light sources showed that compared with the linear 460 nm blue light source, the trapping rate of the L. striatellus was significantly improved by the polychromatic light, and the blue–green light led to the best improvement, reaching 1.5 times that of the trapping rate in the case of monochromatic light sources. The wavelength composition, light intensity, shape, and flash frequency of the light source used in this study can provide a theoretical basis for the development of LED-based light traps specifically for L. striatellus.
RrTTG1 promotes fruit prickle development through an MBW complex in Rosa roxburghii
Authors: Huang Xiaolong , Yi Peipei , Liu Yanjing , Li Qiaohong , Jiang Yu , Yi Yin , Yan Huiqing
Published: August 2022

Fruit prickles are widely distributed on the pericarp and exhibit polymorphic traits at different developmental stages. Although they are multicellular appendages that are well-known for helping plants defend against biotic and abiotic stresses, their origination and molecular mechanism are still less known. Here, we studied the origination and molecular mechanism of fruit prickles in Rosa roxburghii. Using morphological and histological observations, we found that the fruit prickle primordium of R. roxburghii originated from the ground meristem that underwent cell division to form flagelliform prickles, continued to enlarge, and finally lignified to form mature fruit prickles. We amplified a homolog of candidate gene TRANSPARENT TESTA GLABRA1 (TTG1) from R. roxburghii, named RrTTG1. RrTTG1 harbored four conserved WD-repeat domains and was exclusively nuclear-localized. Using qRT-PCR and in situ hybridization, we found that RrTTG1 was constitutively expressed and highly expressed during the initiation and cell expansion phases of fruit prickles. Ectopic expression analysis in Arabidopsis proved that RrTTG1 substantially enhanced the number of trichome and pigmentation production and inhibited root hair formation. Besides, RrTTG1 complemented the phenotypes of the ttg1 mutant in Arabidopsis, thus indicating that RrTTG1 played pleiotropic roles akin to AtTTG1. We demonstrated that the RrTTG1 only interacted with RrEGL3, a homolog of ENHANCER OF GLABRA3 (EGL3), via yeast two-hybrid (Y2H) and bimolecular fluorescence complementation (BiFC) assays. Briefly, RrTTG1 might positively regulate the initiation of fruit prickle primordium and cell enlargement by forming the RrTTG1-RrEGL3-RrGL1 complex in R. roxburghii. Therefore, our results help characterize the RrTTG1 in R. roxburghii and also elucidate the establishment of the prickles regulatory system in the Rosaceae plants.
The haustorium: The root of biotrophic fungal pathogens
Authors: Mapuranga Johannes , Zhang Lirong , Zhang Na , Yang Wenxiang
Published: August 2022

Biotrophic plant pathogenic fungi are among the dreadful pathogens that continuously threaten the production of economically important crops. The interaction of biotrophic fungal pathogens with their hosts necessitates the development of unique infection mechanisms and involvement of various virulence-associated components. Biotrophic plant pathogenic fungi have an exceptional lifestyle that supports nutrient acquisition from cells of a living host and are fully dependent on the host for successful completion of their life cycle. The haustorium, a specialized infection structure, is the key organ for biotrophic fungal pathogens. The haustorium is not only essential in the uptake of nutrients without killing the host, but also in the secretion and delivery of effectors into the host cells to manipulate host immune system and defense responses and reprogram the metabolic flow of the host. Although there is a number of unanswered questions in this area yet, results from various studies indicate that the haustorium is the root of biotrophic fungal pathogens. This review provides an overview of current knowledge of the haustorium, its structure, composition, and functions, which includes the most recent haustorial transcriptome studies.
Design and simulation of a gripper structure of cluster tomato based on manual picking behavior
Authors: Zheng Yifeng , Pi Jie , Guo Tiezheng , Xu Lei , Liu Jun , Kong Jie
Published: August 2022

Picking robot technology plays an important role in the rapid promotion of precision agriculture. The development of a successful robot gripper is critical for the final promotion and industrialization of the tomato picking robot. This paper investigated the cluster tomato picking strategy and the gripper structure design based on this strategy to address the problem of poor adaptability of the existing gripper design in the cluster tomato picking scene. Starting from the research on the behavior of artificially picking cluster tomatoes, the grasping method, finger structure parameters and picking movement pattern of the human hand are analyzed. The evaluation criteria of the gripper are summarized, a simplified mathematical model of the gripper is established, and the picking strategy under the model of the gripper is proposed. Furthermore, according to the simplified gripper model, a rigid-flexible coupling gripper structure is designed, and the gripping simulation analysis is carried out. According to the simulation results, the gripper can smoothly grab medium and large tomatoes with diameter of 65∼95 mm. The peak force and fluctuation force of tomato with different sizes during grasping were less than the tomato’s minimum damage force. The gripper has adaptability and stability characteristics, providing technical support for gripper manufacturing and the construction of a picking system for a tomato picking robot.
Field pest monitoring and forecasting system for pest control
Authors: Liu Chengkang , Zhai Zhiqiang , Zhang Ruoyu , Bai Jingya , Zhang Mengyun
Published: August 2022

Insect pest is an essential factor affecting crop yield, and the effect of pest control depends on the timeliness and accuracy of pest forecasting. The traditional method forecasts pest outbreaks by manually observing (capturing), identifying, and counting insects, which is very time-consuming and laborious. Therefore, developing a method that can more timely and accurately identify insects and obtain insect information. This study designed an image acquisition device that can quickly collect real-time photos of phototactic insects. A pest identification model was established based on a deep learning algorithm. In addition, a model update strategy and a pest outbreak warning method based on the identification results were proposed. Insect images were processed to establish the identification model by removing the background; a laboratory image collection test verified the feasibility. The results showed that the proportion of images with the background completely removed was 90.2%. Dataset 1 was obtained using reared target insects, and the identification accuracy of the ResNet V2 model on the test set was 96%. Furthermore, Dataset 2 was obtained in the cotton field using a designed field device. In exploring the model update strategy, firstly, the T_ResNet V2 model was trained with Dataset 2 using transfer learning based on the ResNet V2 model; its identification accuracy on the test set was 84.6%. Secondly, after reasonably mixing the indoor and field datasets, the SM_ResNet V2 model had an identification accuracy of 85.7%. The cotton pest image acquisition, transmission, and automatic identification system provide a good tool for accurately forecasting pest outbreaks in cotton fields.
Genome-wide identification of wheat ABC1K gene family and functional dissection of TaABC1K3 and TaABC1K6 involved in drought tolerance
Authors: Gao Xiaoran , Zou Rong , Sun Haocheng , Liu Junxian , Duan Wenjing , Hu Yingkao , Yan Yueming
Published: August 2022

Activity of BC1 complex kinase (ABC1K) serves as an atypical kinase family involved in plant stress resistance. This study identified 44 ABC1K genes in the wheat genome, which contained three clades (I–III). TaABC1K genes generally had similar structural features, but differences were present in motif and exon compositions from different clade members. More type II functional divergence sites were detected between clade I and clade III and no positive selection site were found in TaABC1K family. The three-dimensional structure prediction by Alphafold2 showed that TaABC1K proteins had more α-helixes with a relatively even distribution, and different clade members had differences in the content of secondary structures. The cis-acting element analysis showed that TaABC1K genes contained abundant cis-acting elements related to plant hormones and environmental stress response in the promoter region, and generally displayed a significantly upregulated expression under drought stress. In particular, both TaABC1K3 and TaABC1K6 genes from clade I was highly induced by drought stress, and their overexpression in yeast and Arabidopsis enhanced drought tolerance by suppressing active oxygen burst and reducing photosynthesis impairment. Meanwhile, TaABC1K3 and TaABC1K6 could, respectively, complement the function of Arabidopsis abc1k3 and abc1k6 mutants and reduce photosynthesis damage caused by drought stress.
State Boredom Partially Accounts for Gender Differences in Novel Lexicon Learning
Authors: Wang Hua , Xu Yong , Song Hongwen , Mao Tianxin , Huang Yan , Xu Sihua , Zhang Xiaochu , Rao Hengyi
Published: August 2022

Gender plays an important role in various aspects of second language acquisition, including lexicon learning. Many studies have suggested that compared to males, females are less likely to experience boredom, one of the frequently experienced deactivating negative emotions that may impair language learning. However, the contribution of boredom to gender-related differences in lexicon learning remains unclear. To address this question, here we conducted two experiments with a large sample of over 1,000 college students to explore the relationships between gender differences in boredom and lexicon learning. In Experiment 1, a cohort of 527 participants (238 males) completed the trait and state boredom scales as well as a novel lexicon learning task without awareness of the testing process. In Experiment 2, an independent cohort of 506 participants (228 males) completed the same novel lexicon learning task with prior knowledge of the testing procedure. Results from both experiments consistently showed significant differences between female and male participants in the rate of forgetting words and the state boredom scores, with female participants performing better than male participants. Furthermore, differences in state boredom scores partially explained differences in the rate of forgetting words between female and male participants. These findings demonstrate a novel contribution of state boredom to gender differences in lexicon learning, which provides new insights into better language-learning ability in females.
EmotionBox: A music-element-driven emotional music generation system based on music psychology
Authors: Zheng Kaitong , Meng Ruijie , Zheng Chengshi , Li Xiaodong , Sang Jinqiu , Cai Juanjuan , Wang Jie , Wang Xiao
Published: August 2022

With the development of deep neural networks, automatic music composition has made great progress. Although emotional music can evoke listeners' different auditory perceptions, only few research studies have focused on generating emotional music. This paper presents EmotionBox -a music-element-driven emotional music generator based on music psychology that is capable of composing music given a specific emotion, while this model does not require a music dataset labeled with emotions as previous methods. In this work, pitch histogram and note density are extracted as features that represent mode and tempo, respectively, to control music emotions. The specific emotions are mapped from these features through Russell's psychology model. The subjective listening tests show that the Emotionbox has a competitive performance in generating different emotional music and significantly better performance in generating music with low arousal emotions, especially peaceful emotion, compared with the emotion-label-based method.
Extending repair in peer interaction: A conversation analytic study
Authors: Chen Mia Huimin , Ye Shelly Xueting
Published: August 2022

Peer interaction constitutes a focal site for understanding learning orientations and autonomous learning behaviors. Based on 10 h of video-recorded data collected from small-size conversation-for-learning classes, this study, through the lens of Conversation Analysis, analyzes instances in which L2 learners spontaneously exploit learning opportunities from the on-task public talk and make them relevant for private learning in sequential private peer interaction. The analysis of extended negation-for-meaning practices in peer interaction displays how L2 learners orient to public repair for their learning opportunities in an immediate manner and in so doing, how different participation framework is being utilized to maximize their learning outcomes. As these extended repair practices are entirely managed by learners themselves, they yield both efficient and inefficient learning outcomes. Findings reveal that learners frequently resort to their peers to recycle the focal trouble words for learning opportunities, shifting their participating role from the on looking audience to active learners. By reporting the rather under-researched post-repair negotiation-for-meaning sequence in peer interactions, the study highlights the relevance between on-task classroom activities and private learning, contributing to understanding private learning behaviors in the language classroom and learning as a co-constructed activity locally situated in peer interaction.
Superlatives, clickbaits, appeals to authority, poor grammar, or boldface: Is editorial style related to the credibility of online health messages?
Authors: Greškovičová Katarína , Masaryk Radomír , Synak Nikola , Čavojová Vladimíra
Published: August 2022

Adolescents, as active online searchers, have easy access to health information. Much health information they encounter online is of poor quality and even contains potentially harmful health information. The ability to identify the quality of health messages disseminatedviaonline technologies is needed in terms of health attitudes and behaviors. This study aims to understand how different ways of editing health-related messages affect their credibility among adolescents and what impact this may have on the content or format of health information. The sample consisted of 300 secondary school students (Mage= 17.26; SDage= 1.04; 66.3% female). To examine the effects of manipulating editorial elements, we used seven short messages about the health-promoting effects of different fruits and vegetables. Participants were then asked to rate the message’s trustworthiness with a single question. We calculated second-order variable sensitivity as the derivative of the trustworthiness of a fake message from the trustworthiness of a true neutral message. We also controlled for participants’ scientific reasoning, cognitive reflection, and media literacy. Adolescents were able to distinguish overtly fake health messages from true health messages. True messages with and without editorial elements were perceived as equally trustworthy, except for news with clickbait headlines, which were less trustworthy than other true messages. The results were also the same when scientific reasoning, analytical reasoning, and media literacy were considered. Adolescents should be well trained to recognize online health messages with editorial elements characteristic of low-quality content. They should also be trained on how to evaluate these messages.
Problems and countermeasures associated with intercultural adaptation in international education according to the communication action theory model
Authors: Liu Yanjin , Song Yun , Yan Yun
Published: August 2022

Due to the development of the Chinese economy, the consolidation of national power worldwide, and the increasing frequency of economic and cultural exchanges with foreign countries, the number of people from various countries who travel to China to engage in exchanges has increased significantly. Given the development of economic globalization, the acceleration of the process of educational internationalization represents a general trend in higher education development and a common requirement for universities. In addition to education, international students also experience cross-cultural adaptation, that is, behavioral adaptation or changes that occur in people in response to changes in their home country, whether in the form of external or internal cultural adaptation. For international students, the problem of cross-cultural adaptation not only hinders their learning progress but also affects their psychology and living conditions. This article explains the construction of Habermas’s theory of communication action. According to this theory, the purpose of communication is to coordinate the common actions of actors, and this coordination is achieved through mutual communication, which is mediated by language. The article also discusses the cross-cultural adaptation experienced by international students in Chinese universities and highlights the importance of developing educational services for international students in the context of international education. Studies have shown that female students exhibit slightly worse psychological adaptation than male students and female students have a slightly higher rate of depression rate than male students. An interpersonal study of 120 international students traveling to China found that most of these students (60%) were able to adapt in terms of their interpersonal relationships. To solve the problems associated with cross-cultural adaptation of international students in China, some countermeasures have been proposed, mainly including active participation in interpersonal communication, an enhanced understanding of the new culture, and the amplification of cultural identity.
Dilemma and countermeasure of sustainable leadership in physical education development in southern rural Ningxia, China
Authors: Fu Xiaoya , Zhu Weiqiang
Published: August 2022

Globally, above 1.4 billion adults did not reach the recommended level of physical education in their daily life, thus triple intendent reforms are proposed by the Ministry of Physical Education for the development of sporting leadership in schools, colleges, and universities, which are essentially important for the development of physical and mental health of the students. This article analyzes the situation of lacking sustainable sporting leadership among other factors related to Physical Education (PE) resources in the southern areas of Ningxia. A mixed and multi-method approach was adopted to conduct the study. First, an in-depth but an open-ended qualitative interview with the professionals was carried out, followed by cross-sectional data collected from the respondents in two districts of the southern mountainous area of Ningxia. Moreover, a case study was included to support the phenomenon from a contextual perspective. The study present that PE education needs a modernization and rejuvenation plan to link with PE development and its sustainable execution and implementation for the physical and mental development of the learners. Moreover, it is suggested to strengthen the development of physical education with/through the fields of regional integration of educational resources sharing, cultural elements and integration, latest technological tools, research-based and cultural supported curriculum, and endogenous strength construction to promote the development of school physical education. Furthermore, continuous monitoring and evaluation mechanisms need to be adopted to develop physical education in the region.
Teaching presence predicts cognitive presence in blended learning during COVID-19: The chain mediating role of social presence and sense of community
Authors: Li Ling
Published: August 2022

With the continuous lockdown and staying home strategies of COVID-19, both instructors and learners have met with the presence challenges in language learning. To address the complex and dynamic relationships of different presences in blended learning during COVID-19, based on the Community of Inquiry framework, 215 Chinese English learners were obtained as samples for an empirical test. SPSS 23 and PROCESS for SPSS were utilized to examine the hypotheses. Results indicate that teaching presence (TP) has a significant direct positive impact on social presence (SP), sense of community (SoC), and cognitive presence (CP). SP has a significant positive impact on CP and partially mediates the relationship between TP and CP. SoC is also found to impact CP and partially mediates the relationship between TP and CP. The findings also validate the chain mediating role of SP and SoC between TP and CP. Pedagogical implications are discussed.
Increased digital media use is associated with sleep problems among university students: A study during the COVID-19 pandemic in Japan
Authors: Watanabe Kasumi , Adachi Hiroyoshi , Yamamoto Ryohei , Fujino Ryohei , Ishimaru Daiki , Kanayama Daisuke , Sakagami Yukako , Akamine Shoshin , Marutani Noriko , Mamiya Yoshimasa , Mashita Midori , Nakano Natsuko , Kudo Takashi , Ikeda Manabu
Published: August 2022

This retrospective cohort study investigates the association between the incidence of sleep problems and changes in digital media use among university students during the COVID-19 pandemic. It used data from annual health check-ups performed at a Japanese university in 2019 and 2020. Students undergoing these check-ups were identified to respond to questions about sleep problems, digital media use, breakfast and exercise habits, and stress. In total, 3,869 students were included in the analysis. The association between the incidence of sleep problems in 2020 and the changes in digital media use between 2019 and 2020 was assessed using logistic regression models. The rate of long digital media use (≥ 2 hours) in 2019 was 42.6%, while in 2020 it was 53.6%. Incidence of sleep problems was observed in 244 students (6.3%) in 2020. There were 786 students (20.3%) who used digital media for ≤ 2 h in 2019 and ≥ 2 h in 2020. From the sample, 66 students (8.4%) reported incidence of sleep problems in 2020. Additionally, those respondents who specifically reported increased digital media use between 2019 and 2020 (increased use) where at greater risk (OR: 1.76; 95% CI: 1.21, 2.55) of reporting sleep problems in 2020, even after controlling for other study variables. Thus, this study provides evidence that the incidence of sleep problems has had a significant association with an increase in digital media use among university students throughout the COVID-19 pandemic. These findings highlight the importance of ensuring appropriate digital media use among students for improved quality of sleep.
Rural-urban differences in prevalence of and risk factors for refractive errors among school children and adolescents aged 6–18 years in Dalian, China
Authors: Wang Yachen , Liu Lei , Lu Zhili , Qu Yiyin , Ren Xianlong , Wang Jiaojiao , Lu Yan , Liang Wei , Xin Yue , Zhang Nan , Jin Lin , Wang Lijing , Song Jian , Yu Jian , Zhao Lijun , Ma Xiang , Zhang Lijun
Published: August 2022

Purpose

To assess the prevalence of refractive errors (REs) in school children aged 6–18 years in urban and rural settings in Dalian, Northeast of China.

Methods

This is a school-based cross-sectional survey using multi-stage randomization technique. Six- to eighteen-year-old school children from elementary schools, junior and senior high schools from a rural area and an urban area in Dalian were included in December 2018. All subjects underwent a comprehensive questionnaire and eye examination.

Results

A total of 4,522 school children with 6–18 years of age were investigated. The age, gender-adjusted prevalence of myopia, and anisometropia were 82.71 and 7.27% among the urban students as compared to 71.76% and 5.41% among the rural ones (OR = 1.80, 95 % CI = 1.53 - 2.11,P< 0.001; OR = 1.29, 95 % CI = 1.00–1.67,P= 0.049), respectively. The hyperopia was less common in urban students than in rural ones (5.63vs. 10.21%; OR = 0.54, 95 % CI: 0.43–0.67,P< 0.001). However, there was no significant difference in prevalence of astigmatism between urban (46.07%) and rural (44.69%) participants (OR = 0.96, 95 % CI: 0.84–1.10,P= 0.559). The differences on prevalence of REs were attributed to different social-demographic and physiologic factors.

Conclusions

The students from urban settings are more likely to have myopia and anisometropia but less likely to have hyperopia than their rural counterparts. Although considerable attention had been paid to controlling REs, it is necessary to further consider the urban-rural differences in REs.