bluuebunny/crossref_metadata_2025 huggingface dataset:
Genetic diversity and productivity of Gracilaria coronopifolia as alternative for food resource based on RAPD marker
Authors: Windarsih Gut , DWINITA WIKAN UTAMI  , SITI YURIYAH
Published: January 2020

Abstract. Windarsih G, Utami DW, Yuriyah S. 2019. Genetic diversity and productivity of Gracilaria coronopifolia as alternative for food resource based on RAPD marker. Biodiversitas 20: 3758-3765. Gracilaria coronopifolia is one of potential seaweed as an alternative for food resources, especially for agar source. The information about the genetic profile of G. coronopifolia will be useful for assisting in conservation and breeding efforts. RAPD marker is expected to be utilized for characterization of the species with high productivity based on genetic profile. This research aims to determine the genetic diversity and productivity of G. coronopifolia based on RAPD marker. The samples were collected from Anyer Beach, Banten, Indonesia i.e. Tanjung Tum Beach, Mambruk Hotel Beach, and Patra Comfort Anyer Beach, as well as the cultivation ponds of seaweed in Lontar Village, Banten, Indonesia. Five RAPD primers (OPA-01, OPA-16, OPT-05, OPR-02, and UBC-594) were screened to characterize the genetic profile of samples. The results showed that all primers produced polymorphic bands. Based on the genetic profile, the samples of G. coronopifolia were separated into two main clusters, consisted of group A (Tanjung Tum Beach, Patra Beach, and Mambruk Beach) with a coefficient of similarity of 0.44 and group B (Lontar Village) with a similarity coefficient of 0.43. The sample from Lontar Village was separated outside from the other three samples at the waters of Anyer Beach. The sample from Lontar Village had the highest talus height (18.0 cm) and dry weight (1.250 g/clump). The UBC-594 primer was the most associated with the talus height and dry weight on the band size of 50 bp, thus this primer was assumed can be used as a selection marker for the talus height and dry weight of G. coronopifolia.
Short Communication: Digenea parasites in giant mudskipper (Periopthalmodon schlosseri) in Kuala Lupak Village, South Kalimantan, Indonesia
Authors: Hidayaturrahmah Hidayaturrahmah , HERI BUDI SANTOSO  , DESY NURAPRIANA  , BADRUZSAUFARI  , MUHAMAT
Published: January 2020

Abstract. Hidayaturrahmah, Santoso HB, Nurapriana D, Badruzsaufari, Muhamat. 2019. Short Communication: Digenea parasites in giant mudskipper (Periopthalmodon schlosseri) in Kuala Lupak Village, South Kalimantan, Indonesia. Biodiversitas 20: 3782-3787. Parasites are organisms that impart detrimental effects on their host. Monogenea which are generally ectoparasites and digenea which are endoparasites are the most common parasites which invade fish. This research was aimed to find out the existence of monogenean and digenean parasites as well as their species in giant mudskipper or timpakul fish (Periopthalmodon schlosseri) in Kuala Lupak Village, South Kalimantan, Indonesia. Employing 25 giant mudskipper which was randomly collected from Kuala Lupak Village, South Kalimantan, this study explored the presence of ectoparasites and endoparasites on the mucous, fins, gills, intestines, stomach, and meat of the fish. The results showed that only digenean parasites were found in the giant mudskipper (Periopthalmodon schlosseri). The digenean parasites found to come from Opecoeloides genus and Buchepalidae family, metacercariae cysts, and parasites of the nematode phylum. Monogenea parasites were not found due to low fish-density, fish behavior that is rarely in the water, and adequate nutrition which is the opposite of growth-supporting factors of monogenean. Parasitic infection could occur because the fish eat some intermediate hosts of digenea, such as snails and other fish. Besides, the condition of waters that have been contaminated with digenean eggs allows the life cycle of the digenean to continue in fish.
Analysis of stomach content of piscivorous fishes caught in Danau Panggang Peatland, South Kalimantan, Indonesia
Authors: Sofarini Dini , ENDANG YULI HERAWATI  , MOHAMMAD MAHMUDI  , ASUS MAIZAR SURYANTO HERTIKA  , DIANA ARFIATI  , MUHAMMAD MUSA  , MOHAMAD AMIN  , SUPRIHARYONO
Published: January 2020

Abstract. Sofarini D, Herawati EY, Mahmudi M, Hertika AMS, Arfiati D, Musa M, Amin M, Supriharyono. 2019. Analysis of stomach content of piscivorous fishes caught in Danau Panggang Peatland, South Kalimantan, Indonesia. Biodiversitas 20: 3788-3793. The purpose of this study was to analyze the stomach content profile of piscivorous fishes caught at the reservaat station and the station without activity as The control station, in the Danau Panggang peatland. Sampling was carried out four times and the index of preponderance stomach content was determined in piscivorous fishes. The results showed there were nine fish species in the reservaat area, including Cryptopterus spp., Pangasius nieuwenhuisii, Chana striata, Anabas testudineus, Pangasius sp., Mastecembelus erythrotaenia, Macrognathus aculeatus, Mystus nigriceps, and Mystus nemurus. Their gut was dominated 89% by Chrysophyta, and 37.5% by Diatom and Navicula. In the area without activity, six species of fish were caught, including Cryptopterus spp, Pangasius nieuwenhuisii, Anabas testudineus, Pangasius sp, Mystus nigriceps, and Mystus nemurus, with 83% of their gut dominated by Diatom. The water temperature was 28.07-33.25º C, brightness (6-115 cm), pH of 4.23-6.97, dissolved oxygen (DO) (1.4-6.5 mg/L) and BOD (7.52-24.92 mg/L) i.e., water quality parameters that tend to be incompatible with the persistence of fishes. These limiting factors of peatland water and extreme conditions led to a limitation at availability of natural food for piscivorous fishes.
The impacts of bark harvesting on a population of Encephalartos transvenosus (Limpopo cycad), in Limpopo Province, South Africa
Authors: Bamigboye Samuel , M. PETER TSHISIKHAWE
Published: January 2020

Cycads are the most threatened group of plants in the world and there are a wide range of ecological and anthropological forces responsible for the extinction risk of these taxa. South Africa is a global hotspot of cycad diversity and the country’s cycads are facing high extinction risk. In this study we sampled a population (n=34) of Encephalartos transvenosus Stapf & Burtt Davy, a cycad species endemic to Limpopo Province, South Africa. The population was located on the Soutpansberg mountain range in the Mutale local municipality. A survey was conducted to quantify the threats to the population.  Forty-seven percent of the plants had been damaged by bark harvesting for traditional medicine, and the population showed a mortality rate of 9%. We recommend further studies at a range of Encephalartos transvenosus populations to provide a broader understanding of impacts of harvesting and population trends. We also recommend community based initiatives to enhance the protection of this species in the communities in which they are found.
Activities and molecular characterization of petroleum hydrocarbons degrading rhizobacteria from mangrove plants (Rhizophora sp.) in Kulon Progo, Yogyakarta, Indonesia
Authors: Benget Vivekananda Vinsensius , ENDAH RETNANINGRUM
Published: January 2020

Abstract. Benget VV, Retnaningrum E. 2020. Activities and molecular characterization of petroleum hydrocarbons degrading rhizobacteria from mangrove plants (Rhizophora sp.) in Kulon Progo, Yogyakarta, Indonesia. Biodiversitas 21: 21-27. The increasing number of human population density around mangrove ecosystems has resulted in its pollution, particularly oil spills. Rhizobacteria have metabolic abilities that make them possible to live in polluted environmental conditions and the potential to be biological agents for oil remediation. However, investigations of rhizobacteria especially from rhizospheres of mangrove plants (Rhizophora sp.) and their potential use in remediation remains limited. Therefore, the purposes of this research were to investigate the ability of selected isolate in the degradation of petroleum hydrocarbons and identify its isolate using 16S rRNA gene. Observation of that degradation ability was conducted by inoculating the isolate in Bushnell-Haas Minimal Salt (BHMS) medium containing crude petroleum 0.01 %. After 10 days of incubation, the degradation hydrocarbons were analyzed by measuring Total Petroleum Hydrocarbons (TPH) using Gas Chromatography-Flame Ionization Detector (GC-FID) method. Four rhizobacteria isolate from rhizospheres of mangrove plants (Rhizophora sp), NMKP1, NMKP2, NMKP3, and NMKP4 were analyzed based on their growth in Bushnell-Haas Minimal Salt (BHMS) medium containing crude petroleum 0.01 %. The results revealed that NMKP4 isolate had the highest specific growth rate. In addition, it could degrade those hydrocarbons pollutants as much of 98.72 % within 10 days. Based on molecular characterization showed that NMKP4 isolate was in the same cluster as Pseudomonas sp. CO03.
Productivity of three varieties of local upland rice on swidden agriculture field in Setulang village, North Kalimantan, Indonesia
Authors: OKTIANI PERIDA MERANG  , Lahjie Abubakar  M. , SYAHRIR YUSUF  , RUSLIM YOSEP
Published: January 2020

Abstract. Merang OP, Lahjie AM, Yusuf S, Ruslim Y. 2020. Productivity of three varieties of local upland rice on swidden agriculture field in Setulang village, North Kalimantan, Indonesia. Biodiversitas 21: 49-56. Swidden agriculture field is a dry land used by traditional farmers to cultivate some varieties of local upland rice intercropped with vegetables, tubers, and fruits. This rotational cultivation system utilizes the land for planting such food crops in one year period before it is left for fallow periods for years. This study aimed to assess the productivity of local upland rice varieties (i.e. Langsat rice, Telang Usan rice, and Pimping rice) cultivated on swidden agriculture field in regard to the fallow periods. This study was conducted in Setulang village, Malinau District, North Kalimantan Province and employed purposive sampling method using interviews of selected respondents and field observation. Among three varieties of rice in this study, Langsat rice had the longest fallow period with 17 years while Pimping rice had the shortest fallow period with 13 years, with the maximum production were 2,635 kg ha-1 and 1,670 kg ha-1, respectively. Meanwhile, Telang Usan rice reached the maximum production on fallow period of 15 years with the total production of 2,208 kg ha-1. Overall, of the three types of rice planted, the results show that the longer the fallow period, the higher the rice production and the shorter the fallow period, the lower the production. Each type of rice has a different amount of production, although it is planted during the same fallow period.
Impact of agricultural crop type and hunting on bird communities of two villages in Bandung, West Java, Indonesia
Authors: LUKMANUL HAKIM  , OEKAN S. ABDOELLAH  , PARIKESIT  , WITHANINGSIH SUSANTI
Published: January 2020

Abstract. Hakim L, Abdoellah OS, Parikesit, Withaningsih S. 2020. Impact of agricultural crop type and hunting on bird communities of two villages in Bandung, West Java, Indonesia. Biodiversitas 21: 57-66. Land use change has occurred in the Upper Citarum Watershed over time, converting tropical rain-forest to man-made cash-crop gardens dominate the agricultural landscape, leaving mixed-garden (talun), rice fields and smallholder plantation. This changes species composition, community structure and animal diversity, especially bird species. The objective of this paper was to study bird structure community in rural agricultural landscape in Upper Citarum Watershed. We conducted the survey from February to March 2018. We surveyed birds at 36 point counts (PCs), each PC's surveyed six times for 15 minutes along total 6 km of transects in the agricultural landscape in Sukapura and Resmi Tingal Village, consisting of 12 PCs in mixed-garden, 12 PCs in homegarden and 12 PCs in cash-crop garden. Twenty-nine bird species, 17 families and 1.103 individuals were recorded; 2 species being protected by Indonesian law in all sites, three Javan Island endemics and one species migrant bird species. The insectivorous birds are dominant with 15 species in all land-use types. Passer montanus and Javan munia were the most common birds in all land-use types with 820 of 1.103 individual birds (74,9%) were of two bird species. Mixed-garden had higher bird diversity, species richness, and evenness index than the homegarden and cash-crops garden because of the complexity of vegetation structure and canopy cover stratification. There was a significant decrease in some bird species which in the previous study was dominant to be reduced even to local extinctions such as Oriental White-eye (Zosterops palpebrosus), Crescent-chested Babbler (Stachyris melanothorax) and Western Yellow Wagtail (Motacilla flava). Decreased bamboo-tree garden as Crescent-chested Babbler habitat is suspected to be the cause of declination of this bird population. In addition, unregulated bird hunting is also one of the causes of the declining bird populations, especially birds with market value. Some species such as Oriental White-eye (Zosterops palpebrosus), Sooty-headed Bulbul (Pycnonotus aurigaster), Great Tit (Parus major), Bar-winged prinia (Prinia familiaris), Javan myna (Acridotheres javanicus) and Spotted dove (Streptopelia chinensis) have rapidly decreased population because of bird hunting activities.
Local food diversification of foxtail millet (Setaria italica) cultivars in West Sulawesi, Indonesia: A case study of diversity and local culture
Authors: Ramlah Ramlah , Marcia Bunga Pabendon  , Budi Setiadi Daryono
Published: January 2020

Abstract. Daryono BS, Ramlah, Pabendon MB. 2020. Local food diversification of foxtail millet (Setaria italica) cultivars in West Sulawesi, Indonesia: A case study of diversity and local culture. Biodiversitas 21: 67-73. Foxtail millet (Setaria italica (L.) P.Beauv.) is one of the non-rice cereal crops that has long been domesticated in the world, including West Sulawesi-Indonesia as an alternative food crop instead of rice on local culture. Traditional millet cultivars may become an indispensable part of the local culture and traditions of millet-growing people across Asia over many generations. The existence of this germplasm in West Sulawesi Province, Indonesia has been observed morphologically and showed a close relationship with local culture. A total of six traditional millet cultivars (Tarreang) with different morphological characteristics have been found in this region and still preserved sustainably by the local farmers along with their local culture and traditions. The tradition of Sayyang pattu’duq may become a good example of this relationship. In this tradition, some traditional food is made by the local people like porridge tarreang, jelly tarreang, buras tarreang, jepa golla mamea, jepa anjoroi, dodol tarreang, also widely presented and used as a symbol in important ceremonies, such as celebrations in the month of Muharram, completed Al-Qur'an for children, weddings, births or show the baby's. This information may be useful as guidance for conservation and millet breeding programs in the future.
Biomass and carbon distribution on Imperata cylindrica grasslands
Authors: Syahrinudin Syahrinudin , Denich Manfred , Becker Mathias , Hartati Wahjuni , Vlek Paul LG
Published: January 2020

Abstract. Syahrinudin, Denich M, Becker M, Hartati W, Vleg PLG. 2020. Biomass and carbon distribution on Imperata cylindrica grasslands. Biodiversitas 21: 74-79. Invasive plants can alter the diversity and composition of ecological communities leading to ecosystem change both function and biodiversity issues. Imperata cylindrica is an invasive grass thrives on wide range of soil fertility and climatic condition forming a vast grassland area of this species. Conversion of such lands into agriculture and plantation is costly and high capital demanding. This work was devoted to investigate CO2 mitigation potential of the conversion I. cylindrica grassland to plantations in Sumatra and East Kalimantan, Indonesia. Stratified sampling technique was applied for the determination of biomass and carbon stock of the system studied. Results show that I. cylindrica grassland stored only a relatively small amount of biomass C (5.9-7.6 mg ha-1) implying that conversion into a more C-rich tree-based system would be promising tool to enhance C sequestration. Soil C content at both study sites decreased significantly with depth from the top 15 cm to the 3-m depth and was considerably higher in East Kalimantan than in Sumatra. Provided similar land-use history, the differences in soil C are likely to be related to the level prior to the invasion. Roots of I. cylindrica extended to a maximum depth of only 180 cm, but most of these roots being confined to the topsoil, little organic material is deposited in deeper layers. Despite the dense mat of rhizomes, the extent of C stored in the topsoils was modest. As sequestration is of global concern, the conversion of this grassland into systems with higher biomass accumulation and deeper root penetration is required.
Variation of axillary growth as respond of Morus spp. micropropagation using various concentration of Indonesian local solid substance
Authors: Wulandari Yasinta Ratna Esti , Anggradita Laurensia Danis
Published: January 2020

Abstract. Wulandari YRE, Anggradita LD. 2020. Variation of axillary growth as respond of Morus spp. micropropagation using various concentrations of Indonesian local solid substance. Biodiversitas 21: 80-85. The difficulties of growing Morus spp. makes it become one local plant that hard to cultivate conventionally even though it’s a beneficiary plant. Hence cultivation Morus spp. through tissue culture technique could help growing this plant. This research is aimed to design the optimal condition for micropropagation of local Morus spp. (Morus bombycis var. lembang, M. cathayana, M. multicaulis, and M. alba var. kanva-2) using agar-agar as Indonesian local solid substance. This solid substance is used as its cheap and easy to find compared to other solid substances. This research used MS medium supplemented with 0.1 ppm naphthalene acetic acid + 1.0 ppm benzyl aminopurine and various concentration of agar-agar (0.6%, 0.8%, 1.0%). Growth rate, axillary bud length and number, leaf number, callus formation and contamination were observed in this research. All those concentrations could be used for micropropagation of Morus spp. Agar concentrations of 0.8 and 1.0% showed better results than 0.6% because it showed the highest results.
Chemical composition and antibacterial activities of Capparis spinosa essential oils from Algeria
Authors: Benachour Halima , Ramdani Messaoud , Lograda Takia , Chalard Pierre , Figueredo Gilles
Published: January 2020

Abstract. Benachour H, Ramdani M, Lograda T, Chalard P, Figueredo J. 2020. Chemical composition and antibacterial activities of Capparis spinosa essential oils from Algeria. Biodiversitas 21: 161-169. The essential oils of Capparis spinosa L. collected from six locations in Algeria were obtained by hydro-distillation. The chemical composition of oils was performed by GC-MS. The disc diffusion method is applied for the antibacterial activity. The extraction produced low yield (0.03%). The result of chromatographic analysis (GC/MS) leads to the identification of 33 components; palmitic acid (38.19%), nonanal-n (12.61%), cymene-2,5-dimethoxy-para (8.94%) and octacosane (5.49%) were the major components of these oils. The result of cluster analysis based on essential oils constituents showed the presence of three chemotypes,i.e., the chemotype of Nonanal-n-Cymen 2,5 dimethoxy para-Dodecanal, the chemotype of Nonanal-n-Hexadecanoic acid-tetracosane and the chemotype Tetracosane-n-pentyl furane-2-octacosane. In-vitro antimicrobial activity of caper oils against nine bacterial species showed that the oils have no activity against E. coli and have modest activities against eight other bacterial species tested; however, the desirability test shows that the oils used were not effective on the bacteria tested.
Selection of drought-tolerant local rice cultivars from East Nusa Tenggara, Indonesia during vegetative stage
Authors: Salsinha Yustina Carolina Febrianti , Indradewa Didik , Purwestri Yekti Asih , Rachmawati Diah
Published: January 2020

Abstract. Salsinha Y C F, Indradewa D, Purwestri Y A, Rachmawati D. 2020. Selection of drought-tolerant local rice cultivars from East Nusa Tenggara during vegetative stage. Biodiversitas 21: 170-178. East Nusa Tenggara (NTT), with the predominance of dry climate, has the potential of the plant with high tolerance to drought. This research was conducted to select drought-tolerant rice cultivars. There were eight NTT-rice cultivars tested: Pak morin (P1), Mapan (P2), Pak Mutin (P3), Gogo Fatuhao (P4), Seratus-malam Boawae (P5), Padi-Putih Kuatnana (P6), Padi-Putih Maumere (P7) and Gogo Sikka (P8) with 2 control cultivars tested: Ciherang (drought- susceptible) and IR-64 (drought tolerant). The treatment included were 100% FC (control), 50% FC (moderate) and 25% FC (severe drought) during vegetative stage. The morphological analysis of plant (plant height, number of leaves and tillers and root architecture) showed cultivars P1, P5 and P6 possess the best characteristics for tolerating drought. The physiological analysis (plant photosynthetic rate, stomatal conductance, intercellular CO2-concentration, and transpiration rate) showed cultivar P5 and P6 possess the best characteristics for tolerating drought. The high levels of chlorophyll and carotenoid contents during drought conditions were observed in P5, P6, and P7 cultivars. Based on the relative water content, membrane stability index and proline levels, highest-resistance cultivar was P5 and P7 while the cultivar with high sensitivity to drought stress was P3. Based on the morphophysiological characteristics, it can be concluded that P5, P6, and P7 cultivar have the potential as drought-tolerant rice.
Physiological, biochemical activities of cherelle wilt on three cocoa clones (Theobroma cacao) under two levels of soil fertilities
Authors: HS Endang Sri Dewi.HS , PRAPTO YUDONO  , EKA TARWACA SUSILA PUTRA  , BENITO HERU PURWANTO
Published: January 2020

Abstract. Dewi HS ES, Ydono P, Putra ETS, Purwanto BH. 2020. Physiological, biochemical activities of cherelle wilt on three cocoa clones (Theobroma cacao) under two levels of soil fertilities. Biodiversitas 21: 187-194. Cherelle wilt is one of the diseases caused by physiological disorders. It results in loss of young pods by 70-90%. This study aims to reveal the correlation of physiological, biochemical activities of several cocoa clones with two soil fertility status. The study was conducted using a nested design with two blocks as replications. The soil fertility status was classified based on soil chemical status. This obtained two clusters of soil fertility, namely low soil fertility, high soil fertility. The second factor was cocoa clone, consisting of three clones, namely RCC 70, RCC 71, KKM 22. Variables measured were soil character, biochemistry of fresh, wilting cherelles, physiological activities, cherelle wilt, biochemical activities including sucrose content, reducing sugar, invertase activity. The data obtained were analyzed for their variance (ANOVA) with a 95% confidence level, tested using Tukey’s HSD there were significant differences between treatments. The results showed that high soil fertility status could increase contents of N, P, K, boron in leaves, increase nitrate reductase activity, chlorophyll contents, increase photosynthesis rate, suppress transpiration rate, increase assimilate transport from leaves to cherelle, increase enzyme invertase activity. RCC 70 clone indicated better physiological, biochemical activity, had the smallest percentage of cherelle wilt. Soil fertility status can suppress cherelle wilt by up to 60% through increasing physiological, biochemical activity. The RCC 70 clone shows the lowest cherelle wilt values ​​compared to RCC 71, KKM 22 clones.
Population structure, vegetation composition and economic potentials of Parkia timoriana in Meru Betiri National Park, East Java, Indonesia
Authors: Anik Nur Hidayati  , ERVIZAL A.M. ZUHUD  , NURI ANDARWULAN
Published: January 2020

Abstract. Hidayati AN, Zuhud EAM, Andarwulan N. 2020. Population structure, vegetation composition and economic potentials of Parkia timoriana in Meru Betiri National Park, East Java, Indonesia. Biodiversitas 21: 203-210. Kedawung (Parkia timoriana (DC.) Merr.) is one of rare medicinal plant species because it is only found in a small part of Indonesia. P. timoriana has high ecological and economic potential because it contains various active compounds such as anticancer. This study was aimed to investigate the population structure, vegetation community composition, and distribution, as well as the potential production and economic values of P. timoriana in rehabilitation zone in Meru Betiri National Park, East Java, Indonesia. The methods used were vegetation analysis, direct observation, and in-depth interviews with selected respondents using purposive sampling. We found that P. timoriana population at the study sites was only at tree and pole stages, while no sapling and seedling was found. This condition implies that regeneration of P. timoriana at the study sites was not happening which can threaten its sustainability in the future. P. timoriana dominated the vegetation at the studied sites which were indicated by the highest importance value index. Utilization of P. timoriana by the local community was by selling the seeds to medicinal industries or used as herbal medicine. While having conservation values, the management of P. timoriana stands in Andongrejo Resort also had the potential to generate economic values up to 17.6 billion rupiahs. Considering the great economic potential of P. timoriana and to ensure sustainability, so that nursery, breeding and regeneration of P. timoriana should be made in order to obtain the best quality seeds.
Short Communication: Identification of Leptin gene in crossbred beef cattle
Authors: Anugratama Laksa Ersa , Hartatik Tety
Published: January 2020

Abstract. Anugratama LE, Hartatik T. 2020. Short Communication: Identification of Leptin gene in crossbred beef cattle. Biodiversitas 21: 226-230. Leptin is a gene that affects animal weight. Leptin gene is known to control body weight, feed intake, energy expenditure, immune function, and reproduction. This study aims to identify the diversity of the Leptin gene in crossbred beef cattle, Sumba Ongole cattle, Brahman cross cattle, Bali cattle, buffalo, sheep, and goat by comparing with four GenBank data of cattle. Crossbred beef cattle obtained from Klaten, Central Java, Indonesia. Leptin nucleotide sequences were analyzed using BioEdit to identify Single Nucleotide Polymorphism (SNP). To create amino acid change in Leptin gene, the coding sequence of exon 2 was established using BioEdit ver. 7.0.5. Phylogenetic tree and genetic distance have been analyzed based on the Leptin gene using MEGA 10.1.1 program. The result shows that eight variations of SNP were found in exon 2. The phylogenetic tree represents that crossbreed beef cattle, Sumba Ongole cattle, Brahman cross cattle, Bali cattle, Bos taurus, Bos indicus, Bos frontalis, Bos grunniens, Bubalus bubalis are in the same cluster with various genetic distance. The results of this study are expected to provide genetic information that will be used for further research on the relationship between Leptin gene polymorphisms to animal weight.
First ectomycorrhizal syntheses between Astraeus sirindhorniae and Dipterocarpus alatus (Dipterocarpaceae), pure culture characteristics, and molecular detection
Authors: NUTTIKA SUWANNASAI  , PREEYAPORN DOKMAI  , AKIYOSHI YAMADA  , ROY WATLING  , PHOSRI CHERDCHAI
Published: January 2020

Abstract. Suwannasai N, Dokmai P, Yamada A, Watling R, Phosri C. 2020. First ectomycorrhizal syntheses between Astraeus sirindhorniae and Dipterocarpus alatus (Dipterocarpaceae), pure culture characteristics, and molecular detection. Biodiversitas 21: 231-238. This study provides the first mycorrhization of Astraeus sirindhorniae and its cultural characteristics on nutrient media. An attempt has been made to introduce spore suspension and mycelial inocula of A. sirindhorniae onto seedlings of Dipterocarpus alatus. After 6 months seedlings were harvested, measured for growth and morphological descriptions of the ectomycorrhizas formed with D. alatus seedlings were made. The fungus has increased the growth of D. alatus seedlings. Further, it can be confirmed that the primers designed (GAPK126F/GAPK379R) have been successful when applied for the detection of ectomycorrhizal formation of A. sirindhorniae in vivo.
Phenological behavior of Atlas cedar (Cedrus atlantica) forest to snow and precipitation variability in Boutaleb and Babors Mountains, Algeria
Authors: Missaoui Khaled , Gharzouli Rachid , Djellouli Yamna , Messner Frençois
Published: January 2020

Abstract. Missaoui K, Gharzouli R, Djellouli Y, Messner F. 2020. Phenological behavior of Atlas cedar (Cedrus atlantica)  forest to snow and precipitation variability in Boutaleb and Babors Mountains, Algeria. Biodiversitas 21: 239-245. Understanding the changes in snow and precipitation variability and how forest vegetation response to such changes is very important to maintain the long-term sustainability of the forest. However, relatively few studies have investigated this phenomenon in Algeria. This study was aimed to find out the response of Atlas cedar (Cedrus atlantica (Endl.) G.Manetti ex Carrière) forest in two areas (i.e Boutaleb and Babors Mountains) and their response to the precipitation and snow variability. The normalized difference vegetation index (NDVI) generated from satellite images of MODIS time series was used to survey the changes of the Atlas cedar throughout the study area well as dataset of monthly precipitation and snow of the province of Setif (northeast of Algeria) from 2000 to 2018. Descriptive analysis using Standarized Precipitation Index (SPI) showed the wetter years were more frequent in the past than in the last two decades. The NDVI values changes in both areas with high values were detected in Babors Mountains with statistically significant differences. Our findings showed important difference in Atlas cedar phenology from Boutaleb mountains to Babors Mountains which likely related to snow factor.
Short Communication: Leaf architectural characteristics of Cinnamomum cebuense Kosterm. (Lauraceae) distributed in different geographical locations, taxonomic identification and conservation concerns
Authors: Lillo Edgardo , INOCENCIO E. BUOT JR  , ARCHIEBALD B. MALAKI  , STEVE MICHAEL T. ALCAZAR  , RAAMAH ROSALES  , JOHN LOU B. DIAZ  , BERNARDO R. REDOBLADO  , GLORY GRACE G. GEALON
Published: January 2020

Abstract. Lillo EP, Buot IE Jr., Malaki AB, Alcazar SMT, Rosales R, Diaz JLB, Redoblado BR, Gealon GGG. 2019. Short Communication: Leaf architectural characteristics of Cinnamomum cebuense Kosterm. (Lauraceae) distributed in different geographical locations, taxonomic identification and conservation concerns. Biodiversitas 20: 246-251. Populations of Cinnamomum cebuense Kosterm distributed in three geographic locations were examined in the study. The primary aim of the study was to compare the leaf architectural characteristics of Cinnamomum cebuense Kosterm. (Lauraceae) species distributed in three locations. Specimen morphological characters were described using the Leaf Architecture Working Group (LAWG) Manual. Results showed that the species from Nug-as, Alcoy, Cebu was Cinnamomum cebuense because majority of its leaf architectural characteristics and vein categories were similar to those from Tabunan forest. However, the Cinnamomum species from Mount Nacolod, Southern Leyte was slightly different because there are vein categories and other leaf characteristics not similar to those collected from Tabunan forest. The species as one of the endemic plant species on the island of Cebu need to be conserved and protected as they are endangered in the wild.
The Classification of betel leaves (Piper betle) from 15 ethnics in eastern Indonesia based on phytochemicals fingerprint analysis
Authors: Andrianto Dimas , Husnawati  , Hermita Suci , Haryanti Sari
Published: January 2020

Abstract. Andrianto D, Husnawati, Hermita S, Haryanti S. 2020. Classification of betel leaves (Piper betle) from 15 ethnics in eastern Indonesia based on phytochemicals fingerprint analysis. Biodiversitas 21: 252-257. Betel (Piper betle Linn), also called Golden Heart of Nature, is often used as traditional medicine. Nonetheless, betel plants originated from different places contains different phytochemicals profile, resulting in different utilization across ethnics. The research aimed to classify betel leaves from eastern Indonesia origins based on phytochemical profiles. In this study, the phytochemical profiles of 69 betel leave samples from 15 ethnics in eastern Indonesia were obtained using High-Performance Liquid Chromatography (HPLC) fingerprint analysis. This data was then used to classify the betel leaves samples using Principal Component Analysis (PCA). The results of the analysis show that the betel leaves from Eastern Indonesia can be divided into three clusters. Cluster 1 consisted of betel leaves originated from two ethnics, namely Komoro and Greri, from Papua, while Cluster 2 consisted of those originated of two ethnics, namely Sumber Baba and Demta, both were also from Papua. Cluster 3 consisted of betel leaves originated 11 ethnics, namely Asilulu (Maluku), Balesang (Central Sulawesi), Bungku (Central Sulawesi), Mulong Kuni (Central Sulawesi), Saluan (Central Sulawesi), Tialo (Central Sulawesi), Tolage (Central Sulawesi), Gebe (North Maluku), Makian (North Maluku), Mey Brat (West Papua) and Waigeo (West Papua). The location of P. betle plantation in this research accounts for clusterization of samples, Papua island give the highest biodiversity because we can find all the three cluster in Papua island.
The existence of coprophilous macrofungi in Banyumas, Central Java, Indonesia
Authors: Mumpuni Aris , Ekowati Nuraeni , Joko Wahyono Daniel
Published: January 2020

Abstract. Mumpuni A, Ekowati N, Wahyono DJ. 2020. The existence of coprophilous macrofungi in Banyumas, Central Java, Indonesia. Biodiversitas 21: 282-289. Coprophilous fungi are cosmopolitan that inhabiting herbivorous animal feces. Some of them are edible mushrooms, as well as hallucinogenic psychotropic fungi that can be used in the pharmaceutical industry. Studies on coprophilous fungi in Indonesia have not been widely carried out. Tropical climate conditions in Indonesia including in the Ex- Residence of Banyumas, Central Java Province, Indonesia support the growth and spread of coprophilous fungi and are promoted by the spread of herbivorous livestock evenly in almost all regions that always provide suitable dung substrates for the fungal habitat. Based on this background, the purpose of this preliminary study was to obtain coprophilous fungi genera and find out their dominance in the area. This research used survey method with purposive random sampling and focused on macroscopic fungi. The obtained fungi were identified as macro and micro morphologically. From this study, there were 12 genera which were Panaeolus, Coprinopsis, Stropharia, Tricholoma, Lycoperdon, Ascobolus, Rhodocybe, Conocybe, Bolbitius, Leucocoprinus, Mycena, and Hypoloma. The dominance index of the coprophil fungal genera in the ex-residency Banyumas was 0.329; and the coprophil fungi obtained with the highest frequency of occurrence were Coprinopsis (34.4%) and Panaeolus (30.1%).
The vertical distribution of epiphytic orchids on Schima wallichii trees in a montane forest in West Java, Indonesia
Authors: Fardhani Indra , Torimaru Takeshi , Kisanuki Hiromitsu
Published: January 2020

Abstract. Fardhani I, Torimaru T, Kisanuki H. 2020. The vertical distribution of epiphytic orchids on Schima wallichii trees in a montane forest in West Java, Indonesia. Biodiversitas 21: 290-298. Schima wallichii Choisy. is a mostly montane species native to the island of Java; it grows on degraded land areas and is widely used for forest restoration. We studied the vertical distribution of epiphytic orchids on these trees in montane forest on Mt. Sanggara, West Java, Indonesia. To this end, 40 S. wallichii trees with diameter at breast height (DBH) > 20 cm were chosen haphazardly and their epiphytic orchids were identified The diameter and height of each host tree were measured. The position of each epiphytic orchid on each host tree was allocated to one of five zones using Johansson’s method. In total, 39 epiphytic orchid species were identified on 40 host trees at the study site. There was no significant difference in orchid abundance or species richness between crown zones. However, there were significant differences in orchid abundance and species richness between trunks and crowns. Host tree size (DBH) and the number of branches were positively correlated with orchid abundance and species richness. The numbers of orchids and other epiphytic plants were positively correlated in the mid-crown and outer-crown. S. wallichii trees are essential for the epiphytic orchid community because they produce many branches that are suitable for colonization.
Evaluation of SSR and important agronomical characters of promising mutant lines of soybean
Authors: ASADI  , NURWITA DEWI  , KRISTIANTO NUGROHO  , RERENSTRADIKA TIZAR TERRYANA  , MASTUR  , Puji Lestari
Published: January 2020

Abstract. Asadi, Dewi N, Nugroho K, Terryana RT, Mastur, Lestari P. 2020. Evaluation of SSR and important agronomical characters of promising mutant lines of soybean. Biodiversitas 21: 299-310. Improved soybean (Glycine max (L.) Merr.) varieties resistant to major pest or disease, and in accordance with consumer preferences are important in breeding programs to raise their productivity. Identification of superior promising mutant lines of soybean before releasing them needs multiple environment trials complemented with molecular assay. This study aimed to assess morpho-agronomical and molecular characters using SSR markers of promising mutant lines of soybean (Glycine max (L.) Merr.). A total of 14 SSR markers were used to evaluate 20 mutant lines along with their parental lines and check varieties, and eight different locations were chosen to field evaluation of 11 selected lines induced by gamma-ray. Values of Polymorphism Information Content, allele number, and gene diversity index were high, indicating the great genetic diversity among these mutant lines, and far distant from their parental lines. Phylogenetic tree also supported the distinguishable among gamma ray-induced mutant lines compared to the parental lines. The significant interaction between promising line and environment showed their high adaptability and stable yield in various environments. Biosoy-8 (2.713 ton/ha) and Biosoy-11 (2.631 ton/ha) revealing the high yields lines supported with the molecular information could be potential to be released as new varieties and can direct their efficient utilization for field application or further improvement scheme.
Diversity and potency of indigenous yeast from some palm juices for bioethanol production
Authors: Widyaningrum Trianik , SUHARJONO SUHARJONO  , TRI ARDYATI  , AULANNI’AM AULANNI’AM
Published: January 2020

Abstract. Widyaningrum T, Suharjono S, Ardyati T, Aulanni’am A. 2020. Diversity and potency of indigenous yeast from some palm juices for bioethanol production. Biodiversitas 21: 318-325. Main energy source commonly used by the community comes from fossil energy, especially petroleum. The crisis of energy showed that Indonesia's fossil energy reserves are limited. Based on this fact it is important to develop alternative energy that environmentally friendly and sustain, especially bioethanol. The objective of this research was to diversity and potency of indigenous yeast from palm juice of Arenga pinnata Merr., Cocos nucifera L., Nypa fruticans Wurmb., and Borassus flabellifer L. for bioethanol production. Yeast from juice of the four species of palm was isolated using Yeast Malt Extract Agar (YMEA). Those isolates were screen base on ethanol production in coconut water media. The potential of yeast isolates was identified base on 18S DNA sequence similarity. The four potential isolates of yeast were A3A from A. pinnata, K1A from C. nucifera, N3E from N. fruticans, and S1A from B. flabellifer have highest production of ethanol are 8.6 %, 14.2 %, 9.0 %, and 9.2 % respectively. Four potential isolates of yeast were A3A and N3E, K1A, and S1A have relationship with Saccharomyces cerevisiae NRRL Y-12632T, Pichia manshurica IFO 10726T, and Candida tropicalis ATCC 20615 respectively.
The potential impact of climate change on the distribution pattern of Eusideroxylon zwageri (Bornean Ironwood) in Kalimantan, Indonesia
Authors: Yudaputra Angga , Izu Fijridiyanto  , Wendell P.Cropper Jr.
Published: January 2020

Abstract. Yudaputra A, Fijridiyanto I, Cropper WPJr. 2020. The potential impact of climate change on the distribution pattern of Eusideroxylon zwageri (Bornean Ironwood) in Kalimantan, Indonesia. Biodiversitas 21: 326-333. Eusideroxylon zwageri Teijsm & Binn. is a vulnerable tree species with considerable economic value. The high demand for its wood makes this species vulnerable. Population vulnerabilities of E. zwageri include habitat loss, land-use change, and forest encroachment. An additional potential risk factor is climate change, with a possible increase in temperature of about 2.5 to 10 degrees Fahrenheit over the next century. Climate is considered to be a principal factor that determines the distribution of many species. This study addresses the potential current and future distribution of E. zwageri under climate change. Seven predictor climate variables are selected from 19 climatic variables using VIF (Variance Inflation Factor) to eliminate multicollinearity among variables. The spatial data is prepared using Geographic Information System (GIS). Six species distribution models (RF, SVM, MARS, GAM, GLM) and the ensemble model are applied to understand the potential current geographical distribution of E. zwageri. For risk assessment, the potential future distribution is predicted using the ensemble model only. All models are run using R open-source software. In model evaluation, all models have AUC value >0.80, indicating those models are good predictive models. All predictive models have the TSS >0.60 which means those models having a useful agreement between prediction and real observation. Precipitation seasonality, isothermality, and precipitation of the coldest quarter are the most important model variables that influence the current and future distribution of E. zwageri. Four models (RF, SVM, GAM, GLM) produce similar predictive maps of potential current distribution. MARS produces a slightly different predictive map. The future projection of ensemble model shows that the distribution area is more likely shifted and decreased from the current to 2050 and 2070.
Butterfly as bioindicator for development of conservation areas in Bukit Reban Kambing, Bukit Belading and Bukit Tukau, Johor, Malaysia
Authors: ISMAIL NORRADIHAH , Awg Abdul Rahman Aqilah , Mohamed Maryati , Abu Bakar Mohd Fadzelly , Tokiman Lili
Published: January 2020

Abstract. Ismail N, Rahman A A A, Mohamed M, Abu Bakar M F, Tokiman L. 2020. Butterfly as bioindicator for development of conservation areas in Bukit Reban Kambing, Bukit Belading and Bukit Tukau, Johor, Malaysia. Biodiversitas 21: 334-344. Butterflies are often used as indicator surrogates to evaluate the quality of ecosystems. This is made possible due to their sensitive responses toward environmental fluctuations and habitat changes. Butterflies were collected opportunistically using aerial nets at three hill dipterocarp forests; Bukit Reban Kambing (BRK), Bukit Belading (BB) and Bukit Tukau (BT) of Johor, Malaysia from 8th to 11th March 2016. The objectives of this study are to provide a checklist of butterflies of the hills and evaluate habitat suitability in an effort to support the proposal to gazette the hills as part of the Gunung Ledang protected area. A total of 60 individuals belonging to 23 species, 21 genera, and five families were recorded. Comparing the three hills, BRK recorded the greatest individual and species numbers, followed by BT and BB. BRK recorded the highest Shannon diversity index, H’ (2.272) value, whereas BB presented the highest value of species evenness index, E’ (0.945). MaxEnt modeling of the butterflies in BRK, BB, and BT showed a network of high habitat suitability areas connecting Gunung Ledang and our sampling locations. Despite limited sampling effort, this baseline data could possibly strengthen the effort for gazettement and prevent further pressure from mining activity and land conversion.
Abundance, prey, and activity period of dholes (Cuon alpinus) in Khao Yai National Park, Thailand
Authors: NORASET KHOEWSREE  , KHWANRUTAI CHARASPET  , Sukmasuang Ronglarp - , NUCHARIN SONGSASEN  , MANANYA PLA-ARD  , JIDAPA THONGBANTUM  , WARAPORN KONGCHALOEM  , KHANCHIT SRINOPAWAN
Published: January 2020

Abstract. Khoewsree N, Charaspet K, Sukmasuang R, Songsasen N, Pla-ard M, Thongbantum J, Kongchaloem W, Srinopawan K. 2020. Abundance, prey, and activity period of dholes (Cuon alpinus) in Khao Yai National Park, Thailand. Biodiversitas 21: 345-354. The abundance and occupancy of dholes in Khao Yai National Park are the highest among found carnivorous mammals. This demonstrates the importance of the home range of dholes. From the scat analysis, eight species of mammals that are prey can be identified. The dholes mainly consume ungulates when considering the most percentage of relative biomass consumed were ungulate species. For the number of prey animals consumed by dholes per year, a dhole consumes 25.94 prey animals per year which consist of 6.51 masked palm civets, 6.44 Asian palm civets, 4.55 muntjac, 3.97 lesser mouse-deer, 2.22 large Indian civets, 1.03 wild boars, 0.66 sambar, and 0.56 porcupines. From the daily activity pattern captured by the camera trap, dholes have an average activity period of 14:49 hours. Therefore, they are mostly diurnal. The results of the study confirm that Khao Yai National Park is an important habitat of dholes. To maintain the population of dholes in the area, the most important factor is maintaining the ungulate population in the area, especially muntjac, sambar, lesser mouse-deer, and wild boar by increasing and improving the quality of grassland, austerity of maintenance, garbage and dirt disposal from tourism activities in the area for ecological health, and public relations for dholes’ conservation in the long-term.
Cranefly fauna (Diptera: Limoniidae, Pediciidae, Tipulidae) of the Republic of Mordovia, Russia
Authors: Pilipenko Valentin , ALEXANDER B. RUCHIN  , GENNADY B. SEMISHIN
Published: January 2020

Abstract. Pilipenko VE, Ruchin AB, Semishin GB. 2020. Cranefly fauna (Diptera: Limoniidae, Pediciidae, Tipulidae) of the Republic of Mordovia, Russia. Biodiversitas 21: 355-369. The paper summarizes the Tipuloidea fauna of the Republic of Mordovia, Russia, for the first time. Among the 94 species, 29 species of Limoniidae and 14 species of Tipulidae have not been previously recorded from the region. The family Pediciidae, with five species, is also recorded for the first time. Six species are recorded from Сentral European Russia for the first time: Hexatoma (Hexatoma) fuscipennis (Curtis, 1836), Phylidorea (Phylidorea) bicolor (Meigen, 1804), Dicranomyia (Dicranomyia) omissinervis de Meijere, 1918, Limonia macrostigma (Schummel, 1829), Tricyphona (Tricyphona) unicolor (Schummel, 1829), and Ula (Ula) bolitophila Loew, 1869.