bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of Breastmilk Microbiota and Sialylated Oligosaccharides on the Colonization of Infant Gut Microbial Community and Fecal Metabolome
Authors: Ding Juan , Ouyang Runze , Zheng Sijia , Wang Yanfeng , Huang Yan , Ma Xiao , Zou Yuxin , Chen Rong , Zhuo Zhihong , Li Zhen , Xin Qi , Zhou Lina , Mei Surong , Yan Jingyu , Lu Xin , Ren Zhigang , Liu Xinyu , Xu Guowang
Published: November 2022

The complex microbiota and sialylated oligosaccharides in breastmilk are important bioactive components that affect the gut microbiota. However, the effect of breastmilk microbiota and sialylated oligosaccharides on the gut microbiota during the neonatal period has been largely overlooked. Here, 16S rRNA gene sequencing and metabolomics analysis were applied to the breastmilk and feces of 69 newborns to clarify the link between breastmilk components and the newborn gut. Results showed that Staphylococcus, Enterococcus, and Bacteroides were commonly shared and positively correlated between breastmilk and the neonatal intestine and they were the main bacteria of breastmilk that interacted with the newborn fecal metabolome. Breastmilk Staphylococcus mainly interacted with amino acids, whereas Bacteroides was involved in the tryptophan, nucleotide, and vitamin metabolism. Breastmilk sialylated oligosaccharides were related to Bacteroides and amino acids of the newborn fecal metabolites. Moreover, Bacteroides was related to the interaction between breastmilk 3′-sialyllactose and newborn fecal metabolites in the mediation effect models. Finally, we pointed out that breastmilk Bacteroides was important in the milk–gut interaction, and it was negatively associated with waist circumference in infants aged 1 year. Our study provides a scientific basis for understanding the role of breastmilk in the development of newborn gut microbiota and metabolome.
Recent Updates on Source, Biosynthesis, and Therapeutic Potential of Natural Flavonoid Luteolin: A Review
Authors: Muruganathan Nandakumar , Dhanapal Anand Raj , Baskar Venkidasamy , Muthuramalingam Pandiyan , Selvaraj Dhivya , Aara Husne , Shiek Abdullah Mohamed Zubair , Sivanesan Iyyakkannu
Published: November 2022

Nature gives immense resources that are beneficial to humankind. The natural compounds present in plants provide primary nutritional values to our diet. Apart from food, plants also provide chemical compounds with therapeutic values. The importance of these plant secondary metabolites is increasing due to more studies revealing their beneficial properties in treating and managing various diseases and their symptoms. Among them, flavonoids are crucial secondary metabolite compounds present in most plants. Of the reported 8000 flavonoid compounds, luteolin is an essential dietary compound. This review discusses the source of the essential flavonoid luteolin in various plants and its biosynthesis. Furthermore, the potential health benefits of luteolins such as anti-cancer, anti-microbial, anti-inflammatory, antioxidant, and anti-diabetic effects and their mechanisms are discussed in detail. The activity of luteolin and its derivatives are diverse, as they help to prevent and control many diseases and their life-threatening effects. This review will enhance the knowledge and recent findings regarding luteolin and its therapeutic effects, which are certainly useful in potentially utilizing this natural metabolite.
Functional Nutrients to Ameliorate Neurogenic Muscle Atrophy
Authors: Moresi Viviana , Renzini Alessandra , Cavioli Giorgia , Seelaender Marilia , Coletti Dario , Gigli Giuseppe , Cedola Alessia
Published: November 2022

Neurogenic muscle atrophy is a debilitating condition that occurs from nerve trauma in association with diseases or during aging, leading to reduced interaction between motoneurons and skeletal fibers. Current therapeutic approaches aiming at preserving muscle mass in a scenario of decreased nervous input include physical activity and employment of drugs that slow down the progression of the condition yet provide no concrete resolution. Nutritional support appears as a precious tool, adding to the success of personalized medicine, and could thus play a relevant part in mitigating neurogenic muscle atrophy. We herein summarize the molecular pathways triggered by denervation of the skeletal muscle that could be affected by functional nutrients. In this narrative review, we examine and discuss studies pertaining to the use of functional ingredients to counteract neurogenic muscle atrophy, focusing on their preventive or curative means of action within the skeletal muscle. We reviewed experimental models of denervation in rodents and in amyotrophic lateral sclerosis, as well as that caused by aging, considering the knowledge generated with use of animal experimental models and, also, from human studies.
Structural Activity and HAD Inhibition Efficiency of Pelargonidin and Its Glucoside—A Theoretical Approach
Authors: Praveena Rangasamy , Balasankar Athinarayanan , Aruchamy Kanakaraj , Oh Taehwan , Polisetti Veerababu , Ramasundaram Subramaniyan , Anbazhakan Kandasamy
Published: November 2022

Anthocyanins are an important pharmaceutical ingredient possessing diet regulatory, antioxidant, anticancer, antidiabetic, anti-obesity, antimicrobial, and anti-inflammatory properties. Pelargonidin is an important anthocyanin-based orange-red flavonoid compound used in drugs for treating hypoglycemia, retinopathy, skeletal myopathy, etc. The main sources of pelargonidin are strawberries and food products with red pigmentation. There is a lack of evidence for supporting its use as an independent supplement. In the present study, pelargonidin and pelargonidin-3-O-glucoside are studied for their structural properties using quantum chemical calculations based on density functional theory. The results confirmed that the parent compound and its glycosylated derivative acted as good electron donors. Electrostatic potential, frontier molecular orbitals, and molecular descriptor analyses also substantiated their electron donating properties. Furthermore, based on the probability, a target prediction was performed for pelargonidin and pelargonidin-3-O-glucoside. Hydroxyacyl-coenzyme A dehydrogenase was chosen as an enzymatic target of interest, since the presence work focuses on glucuronidated compounds and their efficacy over diabetes. Possible interactions between these compounds and a target with nominable binding energies were also evaluated. Further, the structural stability of these two compounds were also analyzed using a molecular dynamics simulation.
The New Strategy for Studying Drug-Delivery Systems with Prolonged Release: Seven-Day In Vitro Antibacterial Action
Authors: Skuredina Anna A. , Kopnova Tatiana Yu. , Tychinina Anastasia S. , Golyshev Sergey A. , Le-Deygen Irina M. , Belogurova Natalya G. , Kudryashova Elena V.
Published: November 2022

The new method of antibacterial-drug-activity investigation in vitro is proposed as a powerful strategy for understanding how carriers affect drug action during long periods (7 days). In this paper, we observed fluoroquinolone moxifloxacin (MF) antibacterial-efficiency in non-covalent complexes, with the sulfobutyl ether derivative of β-cyclodextrin (SCD) and its polymer (SCDpol). We conducted in vitro studies on two Escherichia coli strains that differed in surface morphology. It was found that MF loses its antibacterial action after 3–4 days in liquid media, whereas the inclusion of the drug in SCD led to the increase of MF antibacterial activity by up to 1.4 times within 1–5 days of the experiment. In the case of MF-SCDpol, we observed a 12-fold increase in the MF action, and a tendency to prolonged antibacterial activity. We visualized this phenomenon (the state of bacteria, cell membrane, and surface morphology) during MF and MF-carrier exposure by TEM. SCD and SCDpol did not change the drug’s mechanism of action. Particle adsorption on cells was the crucial factor for determining the observed effects. The proteinaceous fimbriae on the bacteria surface gave a 2-fold increase of the drug carrier adsorption, hence the strains with fimbriae are more preferable for the proposed treatment. Furthermore, the approach to visualize the CD polymer adsorption on bacteria via TEM is suggested. We hope that the proposed comprehensive method will be useful for the studies of drug-delivery systems to uncover long-term antibacterial action.
Identification of Heavy Metals and Organic Micropollutants in Drinking Water Sources in Typical Villages and Towns in Northeast China
Authors: Wang Guangyuan , Shen Jimin , Wei Shengyue , Cai Daxing , Liu Jinde
Published: November 2022

This study identified and detected the existence of major pollutants in northeast China. As an alpine region and an agricultural base, this region has representative significance in pollution research. We selected 56 samples from drinking water sources of typical villages and towns, focusing on the analysis of heavy metals and organic micropollutants in northeast China. The analysis results showed that Fe and Mn were the main metal elements exceeding the standard. The exceeding rates were 17.9% and 19.6%. Experiments showed that there were 19 kinds of pesticides, 6 kinds of OPEs, 2 kinds of PAEs, 22 kinds of PPCPs. The detection rate of these 49 kinds of organic micro-pollutants were 1.79~82.14%. The characteristics of organic pollution were extensive and varied. Many underground water samples had high level of micropollutants. The water quality parameters of drinking water sources in villages and towns showed close relation to local geological conditions and agricultural activities. Actions must be taken to control these parameters from the source of pollution.
Chemical Fractionations of Lead and Zinc in the Contaminated Soil Amended with the Blended Biochar/Apatite
Authors: Vuong Truong Xuan , Stephen Joseph , Minh Tu Binh , Nguyen Thu Thuy Thi , Duong Tuan Hung , Pham Dung Thuy Nguyen
Published: November 2022

Heavy metal contamination in agricultural land is an alarming issue in Vietnam. It is necessary to develop suitable remediation methods for environmental and farming purposes. The present study investigated the effectiveness of using peanut shell-derived biochar to remediate the two heavy metals Zn and Pb in laboratory soil assays following Tessier’s sequential extraction procedure. The concentration of heavy metals was analyzed using Inductively coupled plasma mass spectrometry (ICP-MS). This study also compared the effectiveness of the blend of biochar and apatite applied and the mere biochar amendment on the chemical fractions of Pb and Zn in the contaminated agricultural soil. Results have shown that the investigated soil was extremely polluted by Pb (3047.8 mg kg−1) and Zn (2034.3 mg kg−1). In addition, the pH, organic carbon, and electrical conductivity values of amended soil samples increased with the increase in the amendment’s ratios. The distribution of heavy metals in soil samples was in the descending order of carbonate fraction (F2) > residue fraction (F5) > exchangeable fraction (F1) > Fe/Mn oxide fraction (F3) > organic fraction (F4) for Pb and F5  ≈  F2 > F1 > F3 > F4 for Zn. The peanut shell-derived biochar produced at 400 °C and 600 °C amended at a 10% ratio (PB4:10 and PB6:10) could significantly reduce the exchangeable fraction Zn from 424.82 mg kg−1 to 277.69 mg kg−1 and 302.89 mg kg−1, respectively, and Pb from 495.77 mg kg−1 to 234.55 mg kg−1 and 275.15 mg kg−1, respectively, and immobilize them in soil. Amending the biochar and apatite combination increased the soil pH, then produced a highly negative charge on the soil surface and facilitated Pb and Zn adsorption. This study shows that the amendment of biochar and biochar blended with apatite could stabilize Pb and Zn fractions, indicating the potential of these amendments to remediate Pb and Zn in contaminated soil.
Effects of Through-Bond and Through-Space Conjugations on the Photoluminescence of Small Aromatic and Aliphatic Aldimines
Authors: Zhuang Peifeng , Yuan Chang , Bai Yunhao , He Changcheng , Long Jiayu , Tan Hongwei , Wang Huiliang
Published: November 2022

Through-bond conjugation (TBC) and/or through-space conjugation (TSC) determine the photophysical properties of organic luminescent compounds. No systematic studies have been carried out to understand the transition from aromatic TBC to non-aromatic TSC on the photoluminescence of organic luminescent compounds. In this work, a series of small aromatic and aliphatic aldimines were synthesized. For the aromatic imines, surprisingly, N,1-diphenylmethanimine with the highest TBC is non-emissive, while N-benzyl-1-phenylmethanimine and N-cyclohexyl-1-phenylmethanimine emit bright fluorescence in aggregate states. The aliphatic imines are all emissive, and their maximum emission wavelength decreases while the quantum yield increases with a decrease in steric hindrance. The imines show concentration-dependent and excitation-dependent emissions. Theoretical calculations show that the TBC extents in the aromatic imines are not strong enough to induce photoluminescence in a single molecule state, while the intermolecular TSC becomes dominant for the fluorescence emissions of both aromatic and aliphatic imines in aggregate states, and the configurations and spatial conformations of the molecules in aggregate states play a key role in the formation of effective TSC. This study provides an understanding of how chemical and spatial structures affect the formation of TBC and TSC and their functions on the photoluminescence of organic luminescent materials.
Rare Earth Complexes of Europium(II) and Substituted Bis(pyrazolyl)borates with High Photoluminescence Efficiency
Authors: Guo Ruoyao , Zhao Zifeng , Wu Aoben , Li Yuqin , Wang Kezhi , Bian Zuqiang , Liu Zhiwei
Published: November 2022

Rare earth europium(II) complexes based on d-f transition luminescence have characteristics of broad emission spectra, tunable emission colors and short excited state lifetimes, showing great potential in display, lighting and other fields. In this work, four complexes of Eu(II) and bis(pyrazolyl)borate ligands, where pyrazolyl stands for pyrazolyl, 3-methylpyrazolyl, 3,5-dimethylpyrazolyl or 3-trifluoromethylpyrazole, were designed and synthesized. Due to the varied steric hindrance of the ligands, different numbers of solvent molecules (tetrahydrofuran) are participated to saturate the coordination structure. These complexes showed blue-green to yellow emissions with maximum wavelength in the range of 490–560 nm, and short excited state lifetimes of 30–540 ns. Among them, the highest photoluminescence quantum yield can reach 100%. In addition, when the complexes were heated under vacuum or nitrogen atmosphere, they finally transformed into the complexes of Eu(II) and corresponding tri(pyrazolyl)borate ligands and sublimated away.
Thermodynamics and Kinetics of Electron Transfer of Electrode-Immobilized Small Laccase from Streptomyces coelicolor
Authors: Di Rocco Giulia , Battistuzzi Gianantonio , Ranieri Antonio , Bortolotti Carlo Augusto , Borsari Marco , Sola Marco
Published: November 2022

The thermodynamic and kinetic properties for heterogeneous electron transfer (ET) were measured for the electrode-immobilized small laccase (SLAC) from Streptomyces coelicolor subjected to different electrostatic and covalent protein-electrode linkages, using cyclic voltammetry. Once immobilized electrostatically onto a gold electrode using mixed carboxyl- and hydroxy-terminated alkane-thiolate SAMs or covalently exploiting the same SAM subjected to N-hydroxysuccinimide+1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide (NHS-EDC) chemistry, the SLAC-electrode electron flow occurs through the T1 center. The E°′ values (from +0.2 to +0.1 V vs. SHE at pH 7.0) are lower by more than 0.2 V compared to the protein either in solution or immobilized with different anchoring strategies using uncharged SAMs. For the present electrostatic and covalent binding, this effect can, respectively, be ascribed to the negative charge of the SAM surfaces and to deletion of the positive charge of Lys/Arg residues due to amide bond formation which both selectively stabilize the more positively charged oxidized SLAC. Observation of enthalpy/entropy compensation within the series indicates that the immobilized proteins experience different reduction-induced solvent reorganization effects. The E°′ values for the covalently attached SLAC are sensitive to three acid base equilibria, with apparent pKa values of pKa1ox = 5.1, pKa1red = 7.5, pKa2ox = 8.4, pKa2red = 10.9, pKa2ox = 8.9, pKa2red = 11.3 possibly involving one residue close to the T1 center and two residues (Lys and/or Arg) along with moderate protein unfolding, respectively. Therefore, the E°′ value of immobilized SLAC turns out to be particularly sensitive to the anchoring mode and medium conditions.
Photoelectron Properties and Organic Molecules Photodegradation Activity of Titania Nanotubes with CuxO Nanoparticles Heat Treated in Air and Argon
Authors: Konstantinova Elizaveta , Savchuk Timofey , Pinchuk Olga , Kytina Ekaterina , Ivanova Elizaveta , Volkova Lidiya , Zaitsev Vladimir , Pavlikov Alexander , Elizarova Elena
Published: November 2022

Titania is very famous photocatalyst for decomposition of organic pollutants. Its photocatalytic properties significantly depend on the morphology and chemical composition of the samples. Herein, the TiO2 nanotubes/CuxO nanoheterostructures have been synthesized and the effect of heat treatment performed in molecular atmospheres of air and argon on their photoelectrochemical and photocatalytic properties has been studied. The prepared samples have a higher reaction rate constant compared to TiO2 nanotubes in the decomposition reaction of methylene blue molecules. It is established that in argon treated nanoheterostructures, the copper oxide is present in two phases, CuO and Cu2O, while in air treated ones there is only CuO. In the TiO2 nanotubes/CuxO samples, Cu2+ ions and molecular O2− radicals were detected while in TiO2 nanotubes only carbon dangling bond defects are present. The dynamics of O2− radicals under illumination are discussed. It was shown that the TiO2 nanotubes do not exhibit photocatalytic activity under visible light. The mechanism of the photocatalytic reaction on the surface of the TiO2 nanotubes/CuxO samples was proposed. It is assumed that a photocatalytic decomposition of organic molecules under visible light at the surface of the nanoheterostructures under investigation is realized mainly by the reaction of these molecules with photogenerated O2− radicals. The results obtained are completely original and indicate the high promise of the prepared photocatalysts.
MnCo2O4/NiCo2O4/rGO as a Catalyst Based on Binary Transition Metal Oxide for the Methanol Oxidation Reaction
Authors: Askari Mohammad Bagher , Azizi Sadegh , Moghadam Mohammad Taghi Tourchi , Seifi Majid , Rozati Seyed Mohammad , Di Bartolomeo Antonio
Published: November 2022

The demands for alternative energy have led researchers to find effective electrocatalysts in fuel cells and increase the efficiency of existing materials. This study presents new nanocatalysts based on two binary transition metal oxides (BTMOs) and their hybrid with reduced graphene oxide for methanol oxidation. Characterization of the introduced three-component composite, including cobalt manganese oxide (MnCo2O4), nickel cobalt oxide (NiCo2O4), and reduced graphene oxide (rGO) in the form of MnCo2O4/NiCo2O4/rGO (MNR), was investigated by X-ray diffraction (XRD), scanning electron microscope (SEM), and energy-dispersive X-ray (EDX) analyses. The alcohol oxidation capability of MnCo2O4/NiCo2O4 (MN) and MNR was evaluated in the methanol oxidation reaction (MOR) process. The crucial role of rGO in improving the electrocatalytic properties of catalysts stems from its large active surface area and high electrical conductivity. The alcohol oxidation tests of MN and MNR showed an adequate ability to oxidize methanol. The better performance of MNR was due to the synergistic effect of MnCo2O4/NiCo2O4 and rGO. MN and MNR nanocatalysts, with a maximum current density of 14.58 and 24.76 mA/cm2 and overvoltage of 0.6 and 0.58 V, as well as cyclic stability of 98.3% and 99.7% (at optimal methanol concentration/scan rate of 20 mV/S), respectively, can be promising and inexpensive options in the field of efficient nanocatalysts for use in methanol fuel cell anodes.
Short-Term Exposure to MPs and DEHP Disrupted Gill Functions in Marine Bivalves
Authors: Zhou Yanfei , Li Yanping , Lan Wenlu , Jiang Hao , Pan Ke
Published: November 2022

The synergistic impact of microplastics (MPs) and organic pollutants remains poorly understood in the marine environment. This study aimed to assess the toxicity of polypropylene microplastics (PS) and/or di-(2-ethylhexyl) phthalate (DEHP) on marine clams. Both Ruditapes philippinarum and Tegillarca granosa were exposed to PS and DEHP individually and combined at environmentally relevant concentrations for 48 h. The filtration rate, antioxidant enzymes activity, lipid peroxidation, reactive oxygen species accumulation, and histological alterations were evaluated. Our results show that single or co-exposure to MPs and DEHP significantly decreases the filtration rate in both type of clams, but the latter exhibited stronger inhibition effect. Close examination of accumulation of reactive oxygen species and related biomarkers revealed that combined exposure exerts greater oxidative stress in the cells, which causes more serious histopathological damage in the gills of the bivalves. Our study implies that MPs, in synergy with organic pollutants, can be more harmful for marine organisms.
Analysis of Formation Mechanisms of Sugar-Derived Dense Carbons via Hydrogel Carbonization Method
Authors: Chen Liting , Fan Zheqiong , Mao Weiguo , Dai Cuiying , Chen Daming , Zhang Xinghong
Published: November 2022

Four kinds of sugar (glucose, fructose, sucrose, and maltose) were selected as carbon precursors, and corresponding dense carbon products were prepared using a novel hydrogel carbonization method. The carbonization processes of sugar–polyacrylamide (sugar–PAM) hydrogels were studied in detail. The molecular structures in the raw materials were analyzed by proton nuclear magnetic resonance spectroscopy (1H NMR). Samples prepared at different temperatures were characterized by thermogravimetry analysis (TGA) and Fourier-transform infrared (FTIR) spectroscopy. The morphology and microstructure of sugar-derived carbons were confirmed by field-emission scanning electron microscopy (FESEM) and X-ray diffraction (XRD). The results indicated that the sugar solution was surrounded by PAM with a three-dimensional network structure and formed hydrogels in the initial stage. The sugar solution was considered to be separated into nanocapsules. In each nanocapsule, sugar molecules could be limited within the hydrogel via walls formed by PAM chains. The hydroxyl group in the sugar molecules connected with PAM by the hydrogen bond and intermolecular force, which can strengthen the entire hydrogel system. The self-generated pressure of hydrogel constrains the foam of sugar during the heat treatment. Finally, dense carbon materials with low graphitization instead of porous structure were prepared at 1200 °C.
Inhibitory Effect of Select Nitrocompounds and Chlorate against Yersinia ruckeri and Yersinia aleksiciae In Vitro
Authors: Latham Elizabeth A. , Anderson Robin C. , Wottlin Lauren R. , Poole Toni L. , Crippen Tawni L. , Schlosser Wayne D. , Harvey Roger B. , Hume Michael E.
Published: November 2022

Yersinia ruckeri is an important fish pathogen causing enteric redmouth disease. Antibiotics have traditionally been used to control this pathogen, but concerns of antibiotic resistance have created a need for alternative interventions. Presently, chlorate and certain nitrocompounds were tested against Y. ruckeri as well as a related species within the genus, Y. aleksiciae, to assess the effects of these inhibitors. The results reveal that 9 mM chlorate had no inhibitory effect against Y. ruckeri, but inhibited growth rates and maximum optical densities of Y. aleksciciae by 20–25% from those of untreated controls (0.46 h−1 and 0.29 maximum optical density, respectively). The results further reveal that 2-nitropropanol and 2-nitroethanol (9 mM) eliminated the growth of both Y. ruckeri and Y. aleksiciae during anaerobic or aerobic culture. Nitroethane, ethyl nitroacetate and ethyl-2-nitropropionate (9 mM) were less inhibitory when tested similarly. Results from a mixed culture of Y. ruckeri with fish tank microbes and of Y. aleksiciae with porcine fecal microbes reveal that the anti-Yersinia activity of the tested nitrocompounds was bactericidal, with 2-nitropropanol and 2-nitroethanol being more potent than the other tested nitrocompounds. The anti-Yersinia activity observed with these tested compounds warrants further study to elucidate the mechanisms of action and strategies for their practical application.
A Salt-Resistant Sodium Carboxymethyl Cellulose Modified by the Heterogeneous Process of Oleate Amide Quaternary Ammonium Salt
Authors: Jia Zhenfu , Zuo Chengwei , Cai Huishan , Li Xiaojiang , Su Xiaodong , Yin Jierui , Zhang Wenlong
Published: November 2022

In this study, hydrophobic quaternary ammonium intermediate was synthesized by epichlorohydrin (ECH) and oleamide propyl dimethyl tertiary amine (PKO). Sodium carboxymethylcellulose (CMC) was chemically modified by introducing a large number of hydrophobic quaternary ammonium branched chains to improve CMC’s salt resistance, thickening ability, and solubility. The quaternary ammonium salt structure can partially offset the compression double-layer effect of linear polymers in a low-price salt ion solution, which makes CMC more stretchable and helps it obtain a higher viscosity and greater drag-reduction performance. The experiment was mainly divided into three parts: Firstly, we performed an epichlorohydrin and oleic acid PKO reaction, generating an oleic acid chain quaternary ammonium chlorine atom intermediate. Secondly, the etherification reaction between intermediate –Cl and –OH groups of CMC was completed. Finally, the modified CMC was characterized by IR, SEM, and XPS, and the viscosity and the drag-reduction rate were evaluated. After CMC and the intermediate were reacted at a mass ratio of 9:1.8 at 80 °C for 5 h, the new CMC with enhanced thickening ability, salt resistance, and drag-reduction performance was obtained. We found that the apparent viscosity increased by 11%, the drag reduction rate increased by 3% on average, and the dissolution rate was also significantly accelerated, which was ascribed to the introduction of quaternary ammonium cation. Moreover, the oleic acid amide chain increased the repulsive force of the CMC chain to low-priced metal cations in solution and intermolecular repulsive force, which is beneficial to increase the viscosity, salt resistance, and drag-reduction performance.
The Alterations and Roles of Glycosaminoglycans in Human Diseases
Authors: Wang Qingchi , Chi Lianli
Published: November 2022

Glycosaminoglycans (GAGs) are a heterogeneous family of linear polysaccharides which are composed of a repeating disaccharide unit. They are also linked to core proteins to form proteoglycans (PGs). GAGs/PGs are major components of the cell surface and the extracellular matrix (ECM), and they display critical roles in development, normal function, and damage response in the body. Some properties (such as expression quantity, molecular weight, and sulfation pattern) of GAGs may be altered under pathological conditions. Due to the close connection between these properties and the function of GAGs/PGs, the alterations are often associated with enormous changes in the physiological/pathological status of cells and organs. Therefore, these GAGs/PGs may serve as marker molecules of disease. This review aimed to investigate the structural alterations and roles of GAGs/PGs in a range of diseases, such as atherosclerosis, cancer, diabetes, neurodegenerative disease, and virus infection. It is hoped to provide a reference for disease diagnosis, monitoring, prognosis, and drug development.
An Advanced Data Fusion Method to Improve Wetland Classification Using Multi-Source Remotely Sensed Data
Authors: Judah Aaron , Hu Baoxin
Published: November 2022

The goal of this research was to improve wetland classification by fully exploiting multi-source remotely sensed data. Three distinct classifiers were designed to distinguish individual or compound wetland categories using random forest (RF) classification. They were determined, in part, to best use the available remotely sensed features in order to maximize that information and to maximize classification accuracy. The results from these classifiers were integrated according to Dempster–Shafer theory (D–S theory). The developed method was tested on data collected from a study area in Northern Alberta, Canada. The data utilized were Landsat-8 and Sentinel-2 (multi-spectral), Sentinel-1 (synthetic aperture radar—SAR), and digital elevation model (DEM). Classification of fen, bog, marsh, swamps, and upland resulted in an overall accuracy of 0.93 using the proposed methodology, an improvement of 5% when compared to a traditional classification method based on the aggregated features from these data sources. It was noted that, with the traditional method, some pixels were misclassified with a high level of confidence (>85%). Such misclassification was significantly reduced (by ~10%) by the proposed method. Results also showed that some features important in separating compound wetland classes were not considered important using the traditional method based on the RF feature selection mechanism. When used in the proposed method, these features increased the classification accuracy, which demonstrated that the proposed method provided an effective means to fully employ available data to improve wetland classification.
Efficient Hardware Accelerator Design of Non-Linear Optimization Correlative Scan Matching Algorithm in 2D LiDAR SLAM for Mobile Robots
Authors: Hu Ao , Yu Guoyi , Wang Qianjin , Han Dongxiao , Zhao Shilun , Liu Bingqiang , Yu Yu , Li Yuwen , Wang Chao , Zou Xuecheng
Published: November 2022

Simultaneous localization and mapping (SLAM) is the major solution for constructing or updating a map of an unknown environment while simultaneously keeping track of a mobile robot’s location. Correlative Scan Matching (CSM) is a scan matching algorithm for obtaining the posterior distribution probability for the robot’s pose in SLAM. This paper combines the non-linear optimization algorithm and CSM algorithm into an NLO-CSM (Non-linear Optimization CSM) algorithm for reducing the computation resources and the amount of computation while ensuring high calculation accuracy, and it presents an efficient hardware accelerator design of the NLO-CSM algorithm for the scan matching in 2D LiDAR SLAM. The proposed NLO-CSM hardware accelerator utilizes pipeline processing and module reusing techniques to achieve low hardware overhead, fast matching, and high energy efficiency. FPGA implementation results show that, at 100 MHz clock, the power consumption of the proposed hardware accelerator is as low as 0.79 W, while it performs a scan match at 8.98 ms and 7.15 mJ per frame. The proposed design outperforms the ARM-A9 dual-core CPU implementation with a 92.74% increase and 90.71% saving in computing speed and energy consumption, respectively. It has also achieved 80.3% LUTs, 84.13% FFs, and 20.83% DSPs saving, as well as an 8.17× increase in frame rate and 96.22% improvement in energy efficiency over a state-of-the-art hardware accelerator design in the literature. ASIC implementation in 65 nm can further reduce the computing time and energy consumption per scan to 5.94 ms and 0.06 mJ, respectively, which shows that the proposed NLO-CSM hardware accelerator design is suitable for resource-limited and energy-constrained mobile and micro robot applications.
Multi-Perspective Hierarchical Deep-Fusion Learning Framework for Lung Nodule Classification
Authors: Sekeroglu Kazim , Soysal Ömer Muhammet
Published: November 2022

Lung cancer is the leading cancer type that causes mortality in both men and women. Computer-aided detection (CAD) and diagnosis systems can play a very important role for helping physicians with cancer treatments. This study proposes a hierarchical deep-fusion learning scheme in a CAD framework for the detection of nodules from computed tomography (CT) scans. In the proposed hierarchical approach, a decision is made at each level individually employing the decisions from the previous level. Further, individual decisions are computed for several perspectives of a volume of interest. This study explores three different approaches to obtain decisions in a hierarchical fashion. The first model utilizes raw images. The second model uses a single type of feature image having salient content. The last model employs multi-type feature images. All models learn the parameters by means of supervised learning. The proposed CAD frameworks are tested using lung CT scans from the LIDC/IDRI database. The experimental results showed that the proposed multi-perspective hierarchical fusion approach significantly improves the performance of the classification. The proposed hierarchical deep-fusion learning model achieved a sensitivity of 95% with only 0.4 fp/scan.
A New Combination of Radio-Frequency Coil Configurations Using High-Permittivity Materials and Inductively Coupled Structures for Ultrahigh-Field Magnetic Resonance Imaging
Authors: Seo Jeung-Hoon , Jo Young-Seung , Oh Chang-Hyun , Chung Jun-Young
Published: November 2022

In ultrahigh-field (UHF) magnetic resonance imaging (MRI) system, the RF power required to excite the nuclei of the target object increases. As the strength of the main magnetic field (B0 field) increases, the improvement of the RF transmit field (B1+ field) efficiency and receive field (B1− field) sensitivity of radio-frequency (RF) coils is essential to reduce their specific absorption rate and power deposition in UHF MRI. To address these problems, we previously proposed a method to simultaneously improve the B1+ field efficiency and B1− field sensitivity of 16-leg bandpass birdcage RF coils (BP-BC RF coils) by combining a multichannel wireless RF element (MCWE) and segmented cylindrical high-permittivity material (scHPM) comprising 16 elements in 7.0 T MRI. In this work, we further improved the performance of transmit/receive RF coils. A new combination of RF coil with wireless element and HPM was proposed by comparing the BP-BC RF coil with the MCWE and the scHPM proposed in the previous study and the multichannel RF coils with a birdcage RF coil-type wireless element (BCWE) and the scHPM proposed in this study. The proposed 16-ch RF coils with the BCWE and scHPM provided excellent B1+ field efficiency and B1− field sensitivity improvement.
Interactive 3D Force/Torque Parameter Acquisition and Correlation Identification during Primary Trocar Insertion in Laparoscopic Abdominal Surgery: 5 Cases
Authors: Nillahoot Nantida , Pillai Branesh M. , Sharma Bibhu , Wilasrusmee Chumpon , Suthakorn Jackrit
Published: November 2022

Laparoscopic procedures have become indispensable in gastrointestinal surgery. As a minimally invasive process, it begins with primary trocar insertion. However, this step poses the threat of injuries to the gastrointestinal tract and blood vessels. As such, the comprehension of the insertion process is crucial to the development of robotic-assisted/automated surgeries. To sustain robotic development, this research aims to study the interactive force/torque (F/T) behavior between the trocar and the abdomen during the trocar insertion process. For force/torque (F/T) data acquisition, a trocar interfaced with a six-axis F/T sensor was used by surgeons for the insertion. The study was conducted during five abdominal hernia surgical cases in the Department of Surgery, Faculty of Medicine, Ramathibodi Hospital, Mahidol University. The real-time F/T data were further processed and analyzed. The fluctuation in the force/torque (F/T) parameter was significant, with peak force ranging from 16.83 N to 61.86 N and peak torque ranging from 0.552 Nm to 1.76 Nm. The force parameter was observed to positively correlate with procedural time, while torque was found to be negatively correlated. Although during the process a surgeon applied force and torque in multiple axes, for a robotic system, the push and turn motion in a single axis was observed to be sufficient. For minimal tissue damage in less procedural time, a system with low push force and high torque was observed to be advantageous. These understandings will eventually benefit the development of computer-assisted or robotics technology to improve the outcome of the primary trocar insertion procedure.
Proposal of Design and Innovation in the Creation of the Internet of Medical Things Based on the CDIO Model through the Methodology of Problem-Based Learning
Authors: Sarmiento-Rojas Jefferson , Aya-Parra Pedro Antonio , Perdomo Oscar J.
Published: November 2022

The educational framework—Conceive, Design, Implement, and Operate—is part of an international proposal to improve education in the field of engineering, emphasizing how to teach engineering comprehensively, which allows the standardization of skills in professionals as a model for teaching engineering. Moreover, problem-based learning allows students to experiment with challenging situations through cases that simulate natural contexts with their profession. The integration of these two education strategies applied to the Internet of Things (IoT) Education for Industry 4.0 has promoted the generation of teaching challenges. Our education strategy proposes the synergy between laboratory guides and the classroom with the following actions: the content of the topic is presented, followed by the presentation of an issue focused into a realistic context, with practical exercises integrating software and hardware for the deployment of the solution to be reported as a final project. Moreover, undergraduate students in the biomedical engineering area acquired new knowledge about IoT, but at the same time, they may develop skills in the field of programming and structuring different architectures to solve real-world problems. Finally, traditional models of education require new teaching initiatives in the field of biomedical engineering concerning the current challenges and needs of the labor market.
Statistical Analysis of the Impact of COVID-19 on PM2.5 Concentrations in Downtown Quito during the Lockdowns in 2020
Authors: Hernandez Wilmar , Arqués-Orobón Francisco José , González-Posadas Vicente , Jiménez-Martín José Luis , Rosero-Montalvo Paul D.
Published: November 2022

In this paper, a comparative analysis between the PM2.5 concentration in downtown Quito, Ecuador, during the COVID-19 pandemic in 2020 and the previous five years (from 2015 to 2019) was carried out. Here, in order to fill in the missing data and achieve homogeneity, eight datasets were constructed, and 35 different estimates were used together with six interpolation methods to put in the estimated value of the missing data. Additionally, the quality of the estimations was verified by using the sum of squared residuals and the following correlation coefficients: Pearson’s r, Kendall’s τ, and Spearman’s ρ. Next, feature vectors were constructed from the data under study using the wavelet transform, and the differences between feature vectors were studied by using principal component analysis and multidimensional scaling. Finally, a robust method to impute missing data in time series and characterize objects is presented. This method was used to support the hypothesis that there were significant differences between the PM2.5 concentration in downtown Quito in 2020 and 2015–2019.
A Pilot Study of Heart Rate Variability Synchrony as a Marker of Intraoperative Surgical Teamwork and Its Correlation to the Length of Procedure
Authors: Powezka Katarzyna , Pettipher Allan , Hemakom Apit , Adjei Tricia , Normahani Pasha , Mandic Danilo P. , Jaffer Usman
Published: November 2022

Objective: Quality of intraoperative teamwork may have a direct impact on patient outcomes. Heart rate variability (HRV) synchrony may be useful for objective assessment of team cohesion and good teamwork. The primary aim of this study was to investigate the feasibility of using HRV synchrony in surgical teams. Secondary aims were to investigate the association of HRV synchrony with length of procedure (LOP), complications, number of intraoperative glitches and length of stay (LOS). We also investigated the correlation between HRV synchrony and team familiarity, pre- and intraoperative stress levels (STAI questionnaire), NOTECHS score and experience of team members. Methods: Ear, nose and throat (ENT) and vascular surgeons (consultant and registrar team members) were recruited into the study. Baseline demographics including level of team members’ experience were gathered before each procedure. For each procedure, continuous electrocardiogram (ECG) recording was performed and questionnaires regarding pre- and intraoperative stress levels and non-technical skills (NOTECHS) scores were collected for each team member. An independent observer documented the time of each intraoperative glitch. Statistical analysis was conducted using stepwise multiple linear regression. Results: Four HRV synchrony metrics which may be markers of efficient surgical collaboration were identified from the data: 1. number of HRV synchronies per hour of procedure, 2. number of HRV synchrony trends per hour of procedure, 3. length of HRV synchrony trends per hour of procedure, 4. area under the HRV synchrony trend curve per hour of procedure. LOP was inversely correlated with number of HRV synchrony trends per hour of procedure (p < 0.0001), area under HRV synchrony trend curve per hour of procedure (p = 0.001), length of HRV synchrony trends per hour of procedure (p = 0.002) and number of HRV synchronies per hour of procedure (p < 0.0001). LOP was positively correlated with: FS (p = 0.043; R = 0.358) and intraoperative STAI score of the whole team (p = 0.007; R = 0.493). Conclusions: HRV synchrony metrics within operating teams may be used as an objective marker to quantify surgical teamwork. We have shown that LOP is shorter when the intraoperative surgical teams’ HRV is more synchronised.
Collaborative Approaches to Addressing Domestic and Sexual Violence among Black and Minority Ethnic Communities in Southampton: A Case Study of Yellow Door
Authors: Olabanji Oluwatayo Adeola
Published: November 2022

Domestic and sexual abuse have been in the academic discourse for quite some time. In recent years in the United Kingdom, the government, non-governmental organisations (NGOs) and the charity sector have doubled their efforts to tackle this challenge through different approaches. One of these approaches is the establishment of specialist services. A case study of these specialist interventions is two advocacy services within a community-based domestic and sexual abuse charity in Southampton named Yellow Door (YD). In line with the specialist service approach (SSA), the diversity, inclusion and advocacy (DIA) service and the Black and minority ethnic Communities (BME) independent sexual violence advisory (ISVA) service were created to address the needs of the BME community. Through the adoption of the collaboration, prevention and education approach, these services support survivors from this community, professionals and community groups to encourage more disclosures and support clients holistically. Recommendations to encourage more reporting and better ways to improve the needs of clients from BME communities were proposed.
Friction Stir Welding of Ti-6Al-4V Using a Liquid-Cooled Nickel Superalloy Tool
Authors: Tarasov Sergei , Amirov Alihan , Chumaevskiy Andrey , Savchenko Nikolay , Rubtsov Valery E. , Ivanov Aleksey , Moskvichev Evgeniy , Kolubaev Evgeny
Published: November 2022

Friction stir welding (FSW) of titanium alloy was carried out using liquid cooling of the FSW tool made of heat-resistant nickel superalloy. Cooling of the nickel superalloy tool was performed by means of circulating water inside the tool. The FSW joints were characterized by microstructures and mechanical strength. The mechanical strength of the joints was higher than that of the base metal.
Investigating the Leishmania donovani sacp Gene and Its Role in Macrophage Infection and Survival in Mice
Authors: Paulini Kayla , Lypaczewski Patrick , Zhang Wen-Wei , Perera Dilhan J. , Ndao Momar , Matlashewski Greg
Published: November 2022

The protozoan parasite Leishmania donovani is a causative agent of the neglected tropical disease known as visceral leishmaniasis, which can be lethal when untreated. Studying Leishmania viru-lence factors is crucial in determining how the parasite causes disease and identifying new targets for treatment. One potential virulence factor is L. donovani’s abundantly secreted protein: secreted acid phosphatase (SAcP). Whole-genome analysis revealed that the sacp gene was present in three copies in wild type L. donovani. Using CRISPR-Cas9 gene editing; we generated a sacp gene knockout termed LdΔSAcP, which demonstrated a loss of both the SAcP protein and an associated reduction in secreted acid phosphatase activity. Genome sequencing confirmed the precise dele-tion of the sacp gene in LdΔSAcP and identified several changes in the genome. LdΔSAcP demonstrated no significant changes in promastigote proliferation or its ability to infect and survive in macrophages compared to the wildtype strain. LdΔSAcP also demonstrated no change in murine liver infection; however, survival was impaired in the spleen. Taken together these results show that SAcP is not necessary for the survival of promastigotes in culture but may support long-term survival in the spleen. These observations also show that the use of CRISPR gene editing and WGS together are effective to investigate the function and phenotype of complex potential drug targets such as multicopy genes.
Tick Control in a Connected World: Challenges, Solutions, and Public Policy from a United States Border Perspective
Authors: Tiffin Hannah S. , Rajotte Edwin G. , Sakamoto Joyce M. , Machtinger Erika T.
Published: November 2022

Ticks are able to transmit the highest number of pathogen species of any blood-feeding arthropod and represent a growing threat to public health and agricultural systems worldwide. While there are numerous and varied causes and effects of changes to tick-borne disease (re)emergence, three primary challenges to tick control were identified in this review from a U.S. borders perspective. (1) Climate change is implicated in current and future alterations to geographic ranges and population densities of tick species, pathogens they can transmit, and their host and reservoir species, as highlighted by Ixodes scapularis and its expansion across southern Canada. (2) Modern technological advances have created an increasingly interconnected world, contributing to an increase in invasive tick species introductions through the increased speed and frequency of trade and travel. The introduction of the invasive Haemaphysalis longicornis in the eastern U.S. exemplifies the challenges with control in a highly interconnected world. (3) Lastly, while not a new challenge, differences in disease surveillance, control, and management strategies in bordering countries remains a critical challenge in managing ticks and tick-borne diseases. International inter-agency collaborations along the U.S.–Mexico border have been critical in control and mitigation of cattle fever ticks (Rhipicephalus spp.) and highlight the need for continued collaboration and research into integrated tick management strategies. These case studies were used to identify challenges and opportunities for tick control and mitigation efforts through a One Health framework.