ОСОБЛИВОСТІ ЦУКРОЗНИЖУВАЛЬНОЇ ДІЇ СУХОГО ЕКСТРАКТУ З КОРЕНІВ І КОРЕНЕВИЩ ОМАНУ ВИСОКОГО ЗАЛЕЖНО ВІД ДОЗИ
Authors: Ежнед М. А. , Грошовий Т. А. , Горошко О. М. 
Published: January 2016

<p align="center"><strong>features of hypoglycemic effect of dry extract from the Elecampane roots and rhizomes depending on the dose.</strong></p><p><strong>М.А. </strong><strong>Ezhned</strong><strong><sup>1</sup></strong><strong>, Т.А. </strong><strong>Groshovyi</strong><strong><sup>2</sup></strong><strong>, О.М. </strong><strong>Goroshko</strong><strong><sup>1</sup></strong></p><p>BukovinianStateMedical University<sup>1</sup>, Chernivtsi</p><p>I.Ya.GorbachevskyiTernopilStateMedical University<sup>2</sup>, Ternopil</p><p><strong>Summary</strong>: in an experiment on rats was established a dose dependency action of  Elecampane dry extract of roots and rhizomes in 1% of starch with a single use on a glucose load. It was found that the glucose-lowering effect of the dry extract of  theElecampane roots and rhizomes  better manifested at a dose of 0.11 mg /kg.</p><p><strong>Keywords:</strong> elecampane, extract, glucose load, hypoglycemic effect.</p><p><strong>Introduction.</strong> In the structure of endocrine diseases diabetes mellitus (DM) constitutes about 60-70%. The frequency of DM vary from 1,5 to 3% on an average increasing in the developed countries of the world (up to 5-6%).</p><p>Therefore, morbidity of DM remains a big problem both inUkraineand in the world practice. That’s why the search of new medicines is rather topical. Today pharmaceutical market presents a wide store of synthetic medical preparations used for the control and treatment of patients with DM. The plants that may be a potential source of hypoglycemic means and widely used in traditional medicine to prevent DM can be an alternative to synthetic preparations.</p><p>Hypoglycemic effect of 70% extract of <em>Inula viscosa</em> has been determined in the experiment. The following stage of the study is to determine dose-dependent action.</p><p><strong>Objective.</strong> To determine the effect of an increasing dose of a dry extract made of the roots and rhizome of <em>Inula viscosa</em> upon hypoglycemic action.    </p><p><strong>Materials and methods.</strong> A dry extract of the roots and rhizomes of <em>Inula</em><em> </em><em>viscosa</em> was studied in 1% of starch paste. Reference therapeutic dose for a rat was found to be 0,08 - 0,2 mg/kg. The medicinal herbal tea “Arfazetin” (the producer – CC “Liktravy”, Zhytomyr), a single plant medical preparation with a proved hyperglycemic activity, which is registered and allowed to be used in Ukraine, was taken as a preparation for comparison as an infusion in the dose of 24 ml/kg.</p><p>The animals were divided into 7 groups. The 1<sup>st</sup> group was the control, all other groups received a dry extract of <em>Inula</em><em> </em><em>viscosa</em> in the dose of 0,1 mg/kg (2 experimental group); 0,11 mg/kg (3 group); 0,12 mg/kg (4 group); 0,15 mg/kg (5 group); 0,2 mg/kg (6 group); the infusion of the medicinal herbal tea “Arfazetin” in the dose of 24 ml/kg (7 group). Glucose concentration in the blood was detected by means of glucose-oxidase method with the set of reagents of the firm “Filicit-Diagnostika”.  </p><p><strong>Results and discussion.</strong> Intra-peritoneal introduction of glucose in the dose of 3 g/kg resulted in the development of acute hyperglycemia manifested a reliable increase of glucose level in all the groups of animals as compared to the initial data.</p><p>Thus, the initial glucose level in rats was in 2,17 times higher as compared to the control group.</p><p>Hypoglycemic action increased with the increase of <em>Inula</em><em> </em><em>viscosa</em> dose, which was maximal as 46,08% in case of injecting in the dose of 0,11 mg/kg. With an increasing dose of a dry extract of <em>Inula</em><em> </em><em>viscosa</em> hypoglycemic action decreased.</p>Thus, in case of injecting <em>Inula viscosa</em> extract in the dose of 0,12 mg/kg hypoglycemic action was 23,53%, in the dose of 0,15 mg/kg - 24,61% respectively, and in the dose of 0,2 mg/kg there is only a tendency to decreased action which is indicative of unreliability of the results.