bluuebunny/crossref_metadata_2025 huggingface dataset:
Real-Time Estimation of Aerobic Threshold and Exercise Intensity Distribution Using Fractal Correlation Properties of Heart Rate Variability: A Single-Case Field Application in a Former Olympic Triathlete
Authors: Gronwald Thomas , Berk Sander , Altini Marco , Mourot Laurent , Hoos Olaf , Rogers Bruce
Published: May 2021

A non-linear heart rate variability (HRV) index based on fractal correlation properties called alpha1 of Detrended Fluctuation Analysis (DFA-alpha1), has been shown to change with endurance exercise intensity. Its unique advantage is that it provides information about current absolute exercise intensity without prior lactate or gas exchange testing. Therefore, real-time assessment of this metric during field conditions using a wearable monitoring device could directly provide a valuable exercise intensity distribution without prior laboratory testing for different applied field settings in endurance sports. Until of late no mobile based product could display DFA-alpha1 in real-time using off the shelf consumer products. Recently an app designed for iOS and Android devices, HRV Logger, was updated to assess DFA-alpha1 in real-time. This brief research report illustrates the potential merits of real-time monitoring of this metric for the purposes of aerobic threshold (AT) estimation and exercise intensity demarcation between low (zone 1) and moderate (zone 2) in a former Olympic triathlete. In a single-case feasibility study, three practically relevant scenarios were successfully evaluated in cycling, (1) estimation of a HRV threshold (HRVT) as an adequate proxy for AT using Kubios HRV software via a typical cycling stage test, (2) estimation of the HRVT during real-time monitoring using a cycling 6 min stage test, (3) a simulated 1 h training ride with enforcement of low intensity boundaries and real-time HRVT confirmation. This single-case field evaluation illustrates the potential of an easy-to-use and low cost real-time estimation of the aerobic threshold and exercise intensity distribution using fractal correlation properties of HRV. Furthermore, this approach may enhance the translation of science into endurance sports practice for future real-world settings.
Radical Prostatectomy: Sequelae in the Course of Time
Authors: Kesch Claudia , Heidegger Isabel , Kasivisvanathan Veeru , Kretschmer Alexander , Marra Giancarlo , Preisser Felix , Tilki Derya , Tsaur Igor , Valerio Massimo , van den Bergh Roderick C. N. , Fankhauser Christian D. , Zattoni Fabio , Gandaglia Giorgio
Published: May 2021

Objective: Radical prostatectomy (RP) is a frequent treatment for men suffering from localized prostate cancer (PCa). Whilst offering a high chance for cure, it does not come without a significant impact on health-related quality of life. Herein we review the common adverse effects RP may have over the course of time.

Methods: A collaborative narrative review was performed with the identification of the principal studies on the topic. The search was executed by a relevant term search on PubMed from 2010 to February 2021.

Results: Rates of major complications in patients undergoing RP are generally low. The main adverse effects are erectile dysfunction varying from 11 to 87% and urinary incontinence varying from 0 to 87% with a peak in functional decline shortly after surgery, and dependent on definitions. Different less frequent side effects also need to be taken into account. The highest rate of recovery is seen within the first year after RP, but even long-term improvements are possible. Nevertheless, for some men these adverse effects are long lasting and different, less frequent side effects also need to be taken into account. Despite many technical advances over the last two decades no surgical approach can be clearly favored when looking at long-term outcome, as surgical volume and experience as well as individual patient characteristics are still the most influential variables.

Conclusions: The frequency of erectile function and urinary continence side effects after RP, and the trajectory of recovery, need to be taken into account when counseling patients about their treatment options for prostate cancer.
Using Expert Elicitation to Abridge the Welfare Quality® Protocol for Monitoring the Most Adverse Dairy Cattle Welfare Impairments
Authors: Tuyttens Frank A. M. , de Graaf Sophie , Andreasen Sine Norlander , de Boyer des Roches Alice , van Eerdenburg Frank J. C. M. , Haskell Marie J. , Kirchner Marlene K. , Mounier Luc. , Kjosevski Miroslav , Bijttebier Jo , Lauwers Ludwig , Verbeke Wim , Ampe Bart
Published: May 2021

The Welfare Quality® consortium has developed and proposed standard protocols for monitoring farm animal welfare. The uptake of the dairy cattle protocol has been below expectation, however, and it has been criticized for the variable quality of the welfare measures and for a limited number of measures having a disproportionally large effect on the integrated welfare categorization. Aiming for a wide uptake by the milk industry, we revised and simplified the Welfare Quality® protocol into a user-friendly tool for cost- and time-efficient on-farm monitoring of dairy cattle welfare with a minimal number of key animal-based measures that are aggregated into a continuous (and thus discriminative) welfare index (WI). The inevitable subjective decisions were based upon expert opinion, as considerable expertise about cattle welfare issues and about the interpretation, importance, and validity of the welfare measures was deemed essential. The WI is calculated as the sum of the severity score (i.e., how severely a welfare problem affects cow welfare) multiplied with the herd prevalence for each measure. The selection of measures (lameness, leanness, mortality, hairless patches, lesions/swellings, somatic cell count) and their severity scores were based on expert surveys (14–17 trained users of the Welfare Quality® cattle protocol). The prevalence of these welfare measures was assessed in 491 European herds. Experts allocated a welfare score (from 0 to 100) to 12 focus herds for which the prevalence of each welfare measure was benchmarked against all 491 herds. Quadratic models indicated a high correspondence between these subjective scores and the WI (R2= 0.91). The WI allows both numerical (0–100) as a qualitative (“not classified” to “excellent”) evaluation of welfare. Although it is sensitive to those welfare issues that most adversely affect cattle welfare (as identified by EFSA), the WI should be accompanied with a disclaimer that lists adverse or favorable effects that cannot be detected adequately by the current selection of measures.
Sperm Response to in vitro Stress Conditions in Wild and Domestic Species Measured by Functional Variables and ROS Production
Authors: O'Brien Emma , García-Casado Pedro , Castaño Cristina , Toledano-Díaz Adolfo , Bóveda Paula , Santiago-Moreno Julián
Published: May 2021

The domestication process has resulted in profound changes in the reproductive physiology of the animals that might have affected the sperm characteristics and thus their sensitivity to handling and cryopreservation procedures. This work assesses the response of the sperm of domestic and wild ungulates to a cooling storage at 15°C for 20 h followed by incubation at 38.5°C, 5% CO2, for 2 h. In addition, this paper examines the most representative sperm traits to assess their responsiveness to these stress conditions. Sperm samples were collected from domestic and their wild ancestor species: ram, mouflon, buck, Iberian ibex, domestic boar, and wild boar. Sperm motility, viability, mitochondrial membrane status, DNA fragmentation, and reactive oxygen species production were evaluated at the beginning of the experiment, after 20 h of refrigeration at 15°C, and, finally, at 2 h of incubation at 38.5°C. Sperm from all domestic species (ram, buck, and domestic boar) suffered more stress than their wild relatives (mouflon, Iberian Ibex, and wild boar). In pigs, the percentage of intact mitochondria was lower in the domestic species compared to wild boar. In sheep, we found a higher reactive oxygen species production in rams, while in goats, the curvilinear velocity was lower in the domestic species. The PCA (principal components analysis) showed that the motility and their kinetic variables were the most represented variables in the principal components of all species, indicating that they are essential biomarkers for evaluating the stress response. Sperm viability was highlighted as a representative variable for evaluating the stress response in domestic boar, mouflon, ram, and ibex.
RNA-Seq Transcriptome Analysis of Peripheral Blood From Cattle Infected With Mycobacterium bovis Across an Experimental Time Course
Authors: McLoughlin Kirsten E. , Correia Carolina N. , Browne John A. , Magee David A. , Nalpas Nicolas C. , Rue-Albrecht Kevin , Whelan Adam O. , Villarreal-Ramos Bernardo , Vordermeier H. Martin , Gormley Eamonn , Gordon Stephen V. , MacHugh David E.
Published: May 2021

Bovine tuberculosis, caused by infection with members of the Mycobacterium tuberculosis complex, particularly Mycobacterium bovis, is a major endemic disease affecting cattle populations worldwide, despite the implementation of stringent surveillance and control programs in many countries. The development of high-throughput functional genomics technologies, including RNA sequencing, has enabled detailed analysis of the host transcriptome to M. bovis infection, particularly at the macrophage and peripheral blood level. In the present study, we have analysed the transcriptome of bovine whole peripheral blood samples collected at −1 week pre-infection and +1, +2, +6, +10, and +12 weeks post-infection time points. Differentially expressed genes were catalogued and evaluated at each post-infection time point relative to the −1 week pre-infection time point and used for the identification of putative candidate host transcriptional biomarkers for M. bovis infection. Differentially expressed gene sets were also used for examination of cellular pathways associated with the host response to M. bovis infection, construction of de novo gene interaction networks enriched for host differentially expressed genes, and time-series analyses to identify functionally important groups of genes displaying similar patterns of expression across the infection time course. A notable outcome of these analyses was identification of a 19-gene transcriptional biosignature of infection consisting of genes increased in expression across the time course from +1 week to +12 weeks post-infection.
FPGA-Based Hybrid Stepper Motor Drive System Design by Variable Structure Control
Authors: Lai Chiu-Keng , Lin Bo-Wei , Lai Hsiang-Yueh , Chen Guan-You
Published: May 2021

A stepper motor is usually operated in position open-loop control for simplicity. However, in order to improve the transient and steady-state performances of the stepper motor-based drive system, a high performance stepper motor drive system is usually designed to feed the encoder signal back to form a closed-loop system such as a general servo motor drive, and high-performance position and speed loop controller can then be used to overcome the nonlinear characteristics of the motor, such as the cogging force and impacts from external load. On the other hand, the existed cogging force of the hybrid stepper motor must be solved to increase the positioning precision. The variable structure control (VSC) is insensitive to the bounded uncertainty and load disturbance, and has been known as a high-performance controller. A predefined sliding surface is used to shape the system performances, and incorporate with switching control to achieve the robustness property. Thus, we applied the VSC to implement the stepper motor drive system about the position and speed control, and the switching control is used to overcome the cogging force. The field programmable gate array (FPGA) is a good alternative to be used to realize a motor drive system by considering its programmable ability and diverse designing environment. It is easily developed as an intellectual property (IP) for future use or combined as a part of a large control system. This paper showed the procedures to develop the hardware circuits for the variable structure controller, and applied it to stepper motor position and velocity control. Functions such as PI controllers, dq-axis transformation and two-phase space vector space vector modulation (SVPWM) designed for the stepper motor drives are also shown in the paper. The system simulation and hardware circuit realization are based on MATLAB/Simulink, and realized on Altera FPGA. Simulations on MATLAB/Simulink with trapezoidal velocity profile command, and experiments with and without the load added are shown to demonstrate the hardware performances and correctness.
Probabilistic Flight Delay Predictions Using Machine Learning and Applications to the Flight-to-Gate Assignment Problem
Authors: Zoutendijk Micha , Mitici Mihaela
Published: May 2021

The problem of flight delay prediction is approached most often by predicting a delay class or value. However, the aviation industry can benefit greatly from probabilistic delay predictions on an individual flight basis, as these give insight into the uncertainty of the delay predictions. Therefore, in this study, two probabilistic forecasting algorithms, Mixture Density Networks and Random Forest regression, are applied to predict flight delays at a European airport. The algorithms estimate well the distribution of arrival and departure flight delays with a Mean Absolute Error of less than 15 min. To illustrate the utility of the estimated delay distributions, we integrate these probabilistic predictions into a probabilistic flight-to-gate assignment problem. The objective of this problem is to increase the robustness of flight-to-gate assignments. Considering probabilistic delay predictions, our proposed flight-to-gate assignment model reduces the number of conflicted aircraft by up to 74% when compared to a deterministic flight-to-gate assignment model. In general, the results illustrate the utility of considering probabilistic forecasting for robust airport operations’ optimization.
A Web-Based Decision Support System for Aircraft Dispatch and Maintenance
Authors: Koornneef Hemmo , Verhagen Wim J. C. , Curran Richard
Published: May 2021

Aircraft dispatch involves determining the optimal dispatch option when an aircraft experiences an unexpected failure. Currently, maintenance technicians at the apron have limited access to support information and finding the right information in extensive maintenance manuals is a time-consuming task, often leading to technically induced delays. This paper introduces a novel web-based prototype decision support system to aid technicians during aircraft dispatch decision-making and subsequent maintenance execution. A system architecture for real-time dispatch decision support is established and implemented. The developed system is evaluated through a case study in an operational environment by licensed maintenance technicians. The system fully automates information retrieval from multiple data sources, performs alternative identification and evaluation for a given fault message, and provides the technician with on-site access to relevant information, including the related maintenance tasks. The case study indicates a potential time saving of up to 98% per dispatch decision. Moreover, it enables digitalization of the—currently mostly paper-based—dispatch decision process, thereby reducing logistics and paper waste. The prototype is the first to provide operational decision support in the aircraft maintenance domain and addresses the lack of correlation between theory and practice often found in decision support systems research by providing a representative case study. The developed custom parser for SGML-based documents enables efficient identification and extraction of relevant information, vastly contributing to the overall reduction of the decision time.
Effect of Different Water Quality on the Nutritive Value and Chemical Composition of Sorghum bicolor Payenne in Cape Verde
Authors: Mendoza-Grimón Vanessa , Amorós Regla , Fernández-Vera Juan Ramón , Hernádez-Moreno Jose Manuel , Palacios-Díaz María del Pino
Published: May 2021

Cape Verde is a semiarid country where lack of rainfall exacerbates the scarce resources available for livestock which, therefore, make it very vulnerable to climate change. By providing reclaimed water (RW) for irrigation, it is possible to decrease forage importation. Subsurface drip irrigation (SDI) improves health security by preventing contact between water and harvested plants. Sorghum is a water-efficient crop that provides good nutritional value. The aim of this experiment was to study the nutrient and fiber contents of the Sorghum Payenne variety using subsurface (T1) and surface (T2) drip irrigation by RW vs. conventional water (T3) and plant maturity to assure the feasibility of water reuse to produce forage. Ntot–Ptot–Ca–Mg and Na were significantly higher in the RW plants than in the conventional water ones. Ntot–Ptot–K and Fe contents significantly lowered, while Ca–Na and Mn significantly rose as plant maturity increased. All the fiber values meet the Nos. 2 and 3 quality standards, and the Prime and No. 1 for NDF and ADF, respectively. The obtained good forage quality let to avoid the competence of conventional water and to reuse nutrients added by RW. If generalized, this solution would reduce forage importation by improving food sovereignty and farmers’ profitability, and would enhance resilience against climate change effects.
Physical, Nutritional and Functional Properties of Walnuts Genotypes (Juglans regia L.) from Romania
Authors: Iordănescu Olimpia A. , Radulov Isidora , P. Buhan Ioana , Cocan Ileana , Berbecea Adina A. , Popescu Iuliana , Poșta Daniela S. , Camen Dorin , Lalescu Dacian
Published: May 2021

Walnut (Juglans regia L.) is the oldest fruit grown in the world and grows spontaneously almost throughout Romania. In this study, the physical properties and chemical composition of twenty types of walnuts from three different locations in Romania were studied. The physical analyzes that were performed were: walnut dimensions and shape properties (nut diameter, nut length, nut shape and nut size), fruit properties (walnut weight, walnut kernel weight and percentage of kernel) and external properties of walnut (walnut kernel extraction, kernel color and shell roughness). The proximate chemical composition (content of water, ash, lipids, proteins and carbohydrates) was determined by standardized AOAC methods, while fatty compounds were determined by GSMS. Water content ranged from 1.23% to 5.00%, mineral content between 1.31% and 2.49%, lipids content between 56.09% and 66.56% and protein content ranged between 12.73% and 20.413%. Based on these, the carbohydrate content and nutritional value of the 20 walnut samples were calculated. In all samples, saturated palmitic acid (C16:0) and stearic acid (C18:0) as well as unsaturated oleic (C18:1), linoleic (C18:2) and linolenic (C18:3) fatty acids were determined. A statistical analysis was also performed correlating the results obtained from the chemical analyzes of the walnuts.
An Overview of Rice Cultivation in Spain and the Management of Herbicide-Resistant Weeds
Authors: Gómez de Barreda Diego , Pardo Gabriel , Osca José María , Catala-Forner Mar , Consola Silvia , Garnica Irache , López-Martínez Nuria , Palmerín José Antonio , Osuna Maria Dolores
Published: May 2021

Spain is the second highest rice-producing country in the European Union, with approximately 105,000 ha used to grow this crop. The major rice-producing regions in Spain are Andalusia, Extremadura, Catalonia, and Valencia, followed by Aragon and Navarre. The main soil texture throughout Spanish rice areas is silty clay loam, with alkaline soils (pH > 7.5)—except in the Extremadura area (pH = 5.5–6)—and a low organic matter content. Water quality in terms of salinity is acceptable, although in some coastal rice areas salinity issues occasionally appear to be a determining factor for high yield achievement. According to a survey carried out on farmers and technicians, the most problematic weeds found in rice crops today in Spain are Echinochloa spp., Leptochloa spp., and Cyperus difformis. Most of the currently authorized herbicides can be classified according to two modes of action: ALS-inhibiting and ACCase-inhibiting. Repeated field applications of herbicides with the same mode of action have resulted in the selection of herbicide-resistant weeds. At present, resistance has been confirmed in different regions of Spain to ALS inhibitors in Echinochloa spp., Leptochloa spp., and Cyperus difformis, and to ACCase inhibitors in Echinochloa spp. and Leptochloa spp. The mechanism of resistance in these species is a mutation in the target site of these herbicides. Several mutations have been found in the ALS gene, both in Echinochloa spp. and Cyperus difformis, distributed in the different rice-growing regions considered in this work. ACCase gene mutations have been mainly found in Leptochloa spp. individuals from Extremadura and Valencia. These different mutations have resulted in different patterns of cross-resistance to ALS- and ACCase-inhibiting herbicides. It is likely that the repeated use of these two modes of action in rice will result in the evolution of more resistant weed populations. The possible availability of new herbicides with alternative modes of action in a short space of time seems very limited, suggesting the need for a more appropriate use of the available alternative strategies (crop rotation, dry sowing, manual weeding, etc.). This work presents a review of the main characteristics of rice cultivation in Spain, emphasizing the current problems in this crop and the management of herbicide-resistant weeds.
Climate-Smart Agriculture on Small-Scale Farms: A Systematic Literature Review
Authors: Mizik Tamás
Published: May 2021

Overpopulation and climate change are among the greatest challenges the world faces. Climate-smart agriculture (CSA) provides an adequate answer by aiming for higher productivity, resilience, as well as GHG emission reduction. As small-scale farms are the cornerstone of the agricultural sector, especially in developing countries, their greater involvement in climate-related actions is essential. CSA practices seek a higher and more stable income sustainably. This systematic literature review aims to provide an overview of how CSA is realized on small-scale farms, what the major CSA practices applied are, and what factors motivate and hamper higher CSA adoption. Based on 30 selected articles, the major message of the literature is a case/site-specific approach due to the tremendous heterogeneity of small-scale farms. As agricultural production is characterized by high risks and low returns, small-scale farmers must consider the length of the payback period when they decide on any CSA practices. This is the reason smallholdings, who implement any CSA practices, must achieve economic benefits, otherwise, they need to be compensated for providing environmental benefits. Moreover, simpler methods with low labor intensity are often applied. Access to the different financial instruments and inputs, knowledge/education/information, and land use security are the critical factors of the CSA adoption. Furthermore, it is worth mentioning that, unlike off-farm activities/incomes, full-time farming is a serious commitment that positively influences CSA adoption.
The Nutritional Value and Safety of Genetically Unmodified Soybeans and Soybean Feed Products in the Nutrition of Farm Animals
Authors: Świątkiewicz Małgorzata , Witaszek Kamil , Sosin Ewa , Pilarski Krzysztof , Szymczyk Beata , Durczak Karol
Published: May 2021

Post-extraction soybean (Glycine max (L.) Merr.) meal is widely used as a basic protein feed for farm animals, especially poultry and pigs. Products made from unmodified soybean seeds are an alternative to imported GMO soybean meal. The aim of the study was to develop feed products from popular European varieties of genetically unmodified soybeans, which can be produced on small and medium-sized farms, and to assess their nutritional value and safety to livestock. The research was conducted on the seeds of three soybean varieties and two types of feed products resulting from thermobaric treatment (extrudate) and oil pressing (soybean press cake). The mould and yeast contamination of domestic seeds was negligible. The thermobaric and pressing treatments lowered the content of fungi by 97%. The products were considered free from mycotoxins. In comparison with full-fat soybean seeds, the protein content in the products was up to 19% higher, and 92% of the total lysine remained available. The products had lower content of antinutritional ingredients (trypsin inhibitors) and the urease activity was reduced by 52–59% and 99%, respectively. The experiment showed that the European genetically unmodified soybean feed products were characterised by good quality, mycotoxicological purity and high nutritional value for farm animals.
Prebiotic Effects of Seaweed Polysaccharides in Pigs
Authors: Corino Carlo , Di Giancamillo Alessia , Modina Silvia Clotilde , Rossi Raffaella
Published: May 2021

To ensure environmental sustainability, according to the European Green Deal and to boost the One Health concept, it is essential to improve animals’ health and adopt sustainable and natural feed ingredients. Over the past decade, prebiotics have been used as an alternative approach in order to reduce the use of antimicrobials, by positively affecting the gut microbiota and decreasing the onset of several enteric diseases in pig. However, dietary supplementation with seaweed polysaccharides as prebiotics has gained attention in recent years. Seaweeds or marine macroalgae contain several polysaccharides: laminarin, fucoidan, and alginates are found in brown seaweeds, carrageenan in red seaweeds, and ulvan in green seaweeds. The present review focuses on studies evaluating dietary seaweed polysaccharide supplementation in pig used as prebiotics to positively modulate gut health and microbiota composition.
Effects of 25-Hydroxyvitamin D3 and Oral Calcium Bolus on Lactation Performance, Ca Homeostasis, and Health of Multiparous Dairy Cows
Authors: Xu Hongjian , Zhang Quanyu , Wang Lihua , Zhang Chengrui , Li Yang , Zhang Yonggen
Published: May 2021

Little information is available regarding the effect of supplementing 25-hydroxyvitamin D3 during the transition period combined with a postpartum oral calcium bolus on Ca homeostasis. The objectives of the current study were to evaluate the effects of 25-hydroxyvitamin D3 combined with postpartum oral calcium bolus on lactation performance, serum minerals and vitamin D3 metabolites, blood biochemistry, and antioxidant and immune function in multiparous dairy cows. To evaluate the effects of 25-hydroxyvitamin D3 combined with oral calcium, 48 multiparous Holstein cows were randomly assigned to one of four treatments: (1) supplementing 240 mg/day vitamin D3 without a postpartum oral Ca bolus (control), (2) supplementing 240 mg/day vitamin D3 with an oral Ca bolus containing 90 g of Ca immediately post-calving (Ca + VitD), (3) supplementing 6 g/day 25-hydroxyvitamin D3 without an oral Ca bolus (25D), and (4) supplementing 6 g/day 25-hydroxyvitamin D3 with an oral Ca bolus containing 90 g of Ca immediately post-calving (Ca + 25D). Lactation performance during the first 21 days was measured. Blood was collected at the initiation of calving and then 1, 2, 7, 14, and 21 days relative to the calving date. The yield of milk (0.05 < p < 0.10), energy-corrected milk (p < 0.05), 3.5% fat-corrected milk (p < 0.05), and milk protein (p < 0.05) were significantly higher in 25-hydroxyvitamin D3-treated groups within 3 weeks of lactation than in vitamin D3-treated cows. The iCa (p < 0.05) and tCa (p < 0.05) were higher in both Ca and 25D + Ca cows than in the control and 25D groups within 48 h. The concentrations of serum tCa (p < 0.05), tP (p < 0.05), and 25-hydroxyvitamin D3 (p < 0.05) in 25D and 25D + Ca cows were higher than those in control and Ca cows within 21 days postpartum. Feeding 25-hydroxyvitamin D3 also showed a lower concentration of malondialdehyde (p < 0.05), interleukin 6 (p < 0.05), and tumor necrosis factor-alpha (TNF-α) (p < 0.05), as well as a higher concentration of alkaline phosphatase (p < 0.05), total antioxidant capacity (p < 0.05), and immunoglobulin G (p < 0.05) than vitamin D3. Supplementing Ca bolus also showed lower concentrations of alanine transaminase (p < 0.05) and TNF-α (p < 0.05). In conclusion, supplementing 25-hydroxyvitamin D3 during the transition period combined with a postpartum oral calcium bolus improved lactation performance, Ca homeostasis, and antioxidant and immune function of medium-production dairy cows within 21 days postpartum.
Soybean Meal Can Be Replaced by Faba Beans, Pumpkin Seed Cake, Spirulina or Be Completely Omitted in a Forage-Based Diet for Fattening Bulls to Achieve Comparable Performance, Carcass and Meat Quality
Authors: Keller Magdalena , Reidy Beat , Scheurer Andreas , Eggerschwiler Lukas , Morel Isabelle , Giller Katrin
Published: May 2021

The aim of the study was to investigate the complete substitution of imported soybean meal in beef cattle diets and the consequences on performance, meat, and adipose tissue quality. Thirty growing crossbred Limousin bulls, with an initial bodyweight of 164 ± 13 kg and 4.3 ± 0.3 months of age, were fed a grass/maize-silage based diet with little additional concentrate (0.5:0.3:0.2). Concentrates contained either soybean meal (positive control), faba beans, pumpkin seed cake, or spirulina (Arthrospira platensis), resulting in about 226 g crude protein (CP)/kg concentrate dry matter (DM) and 158 g CP/kg total diet DM. A grain-based concentrate providing just 135 g CP/kg concentrate DM and 139 g CP/total diet DM served as a negative control. Bulls of all groups had comparable average daily gains (1.43 ± 0.1 kg) and feed intakes (6.92 ± 0.37 kg). Carcass and meat quality did not differ among groups. The fatty acid profile of meat lipids was hardly affected. These results indicate that soybean meal can be replaced by any of the tested protein sources without impairing performance or meat quality. Importantly, bulls fed the negative control achieved a fattening and slaughter performance comparable to that of the protein-supplemented groups without affecting meat and adipose tissue quality. Thus, the present findings suggest that feeding crossbred bulls a grass/maize-silage based diet does not require additional protein supplementation.
Supplemental Effects of Functional Oils on the Modulation of Mucosa-Associated Microbiota, Intestinal Health, and Growth Performance of Nursery Pigs
Authors: Moita Vitor Hugo C. , Duarte Marcos Elias , da Silva Suelen Nunes , Kim Sung Woo
Published: May 2021

This study aimed to investigate the effects of functional oils on modulation of mucosa-associated microbiota, intestinal health, and growth performance of nursery pigs. Forty newly weaned pigs (20 barrows and 20 gilts) with 7.0 ± 0.5 kg body weight (BW) were housed individually and randomly allotted in a randomized complete block design with sex and initial BW as blocks. The dietary treatments were a basal diet with increasing levels (0.00, 0.50, 0.75, 1.00, and 1.50 g/kg feed) of functional oils (a blend of castor oil and cashew nutshell liquid; Oligo Basics USA LLC, Cary, NC) fed to pigs for 34 days divided in two phases (P1 for 13 days and P2 for 21 days). Growth performance was analyzed weekly. On day 34, all pigs were euthanized to collect jejunal mucosa for analyzing the mucosa-associated microbiota and intestinal health, and ileal digesta for analyzing apparent ileal digestibility. Data were analyzed using SAS 9.4. Supplementation of functional oils did not affect the overall growth performance. Increasing supplementation of functional oils reduced (p < 0.05) the relative abundance of Helicobacteraceae, whereas it increased (p < 0.05) Lactobacillus kitasatonis. Supplementation of functional oils tended (p = 0.064) to decrease protein carbonyl and increase the villus height (p = 0.098) and crypt depth (p = 0.070). In conclusion, supplementation of functional oils enhanced intestinal health of nursery pigs by increasing beneficial and reducing harmful bacteria, potentially reducing oxidative stress and enhancing intestinal morphology, without affecting overall growth performance of pigs. Supplementation of functional oils at 0.75–1.50 g/kg feed was the most beneficial to the jejunal mucosa-associated microbiota and intestinal integrity of nursery pigs.
Effect of Media with Different Glycerol Concentrations on Sheep Red Blood Cells’ Viability In Vitro
Authors: Pasciu Valeria , Sotgiu Francesca D. , Porcu Cristian , Berlinguer Fiammetta
Published: May 2021

The use of high doses of glycerol as a livestock feed supplement is followed by a rapid increase in plasma concentrations and consequently in plasma osmolality. Moreover, glycerol is a highly diffusible molecule that can readily permeate the red blood cell (RBC) membrane following a concentration gradient. A rise in glycerol plasma concentrations can thus alter RBC homeostasis. The present study aimed at investigating both glycerol osmotic effects on sheep RBCs and their oxidative response under in vitro conditions. Sheep blood samples were suspended in media supplemented with increasing glycerol concentrations (0, 25, 50, 100, 150, 200, 250, 300, 350, 400 mg/dL), which reflected those found in vivo in previous studies, and incubated at 37 °C for 4h. Thereafter, osmolality and hemolysis were determined in spent media, while cell extracts were used to assay intracellular concentration of glycerol, ATP, Ca2+ ions, oxidative stress markers and reactive oxygen species (ROS).The study confirmed that glycerol intracellular concentrations are directly related with its concentration in the incubation media, as well as hemolysis (p < 0.001) which increased significantly at glycerol concentrations higher form 200 mg/dL. ROS intracellular level increased at all glycerol concentration tested (p < 0.01) and total thiols decreased at the highest concentrations. However, RBCs proved to be able to cope by activating their antioxidant defense system. Superoxide dismutase activity indeed increased at the highest glycerol concentrations (p < 0.001), while total antioxidant capacity and malonyldialdehyde, a typical product of lipid peroxidation by ROS, did not show significant changes. Moreover, no alterations in intracellular Ca2+ ions and ATP concentrations were found. In conclusion, glycerol-induced hemolysis can be related to the induced osmotic stress. In sheep, nutritional treatments should be designed to avoid reaching glycerol circulating concentrations higher than 200 mg/dL.
Effect of Chitosan and Naringin on Enteric Methane Emissions in Crossbred Heifers Fed Tropical Grass
Authors: Jiménez-Ocampo Rafael , Montoya-Flores María Denisse , Herrera-Torres Esperanza , Pámanes-Carrasco Gerardo , Arceo-Castillo Jeyder Israel , Valencia-Salazar Sara Stephanie , Arango Jacobo , Aguilar-Pérez Carlos Fernando , Ramírez-Avilés Luis , Solorio-Sánchez Francisco Javier , Piñeiro-Vázquez Ángel Trinidad , Ku-Vera Juan Carlos
Published: May 2021

In order to meet consumer needs, the livestock industry is increasingly seeking natural feed additives with the ability to improve the efficiency of nutrient utilization, alternatives to antibiotics, and mitigate methane emissions in ruminants. Chitosan (CHI) is a polysaccharide with antimicrobial capability against protozoa and Gram-positive and -negative bacteria, fungi, and yeasts while naringin (NA) is a flavonoid with antimicrobial and antioxidant properties. First, an in vitro gas production experiment was performed adding 0, 1.5, 3.0 g/kg of CHI and NA under a completely randomized design. The substrate containing forage and concentrate in a 70:30 ratio on a dry matter (DM) basis. Compounds increased the concentration of propionic acid, and a significant reduction in methane production was observed with the inclusion of CHI at 1.5 g/kg in in vitro experiments (p < 0.001). In a dry matter rumen degradability study for 96 h, there were no differences in potential and effective degradability. In the in vivo study, six crossbred heifers fitted with rumen cannulas were assigned to a 6 × 6 Latin square design according to the following treatments: control (CTL), no additive; chitosan (CHI1, 1.5 g/kg DMI); (CHI2, 3.0 g/kg DMI); naringin (NA1, 1.5 g/kg DMI); (NA2, 3.0 g/kg DMI) and a mixture of CHI and NA (1.5 + 1.5 g/kg DMI) given directly through the rumen cannula. Additives did not affect rumen fermentation (p > 0.05), DM intake and digestibility of (p > 0.05), and enteric methane emissions (p > 0.05). CHI at a concentration of 1.5 g/kg DM in in vitro experiments had a positive effect on fermentation pattern increasing propionate and reduced methane production. In contrast, in the in vivo studies, there was not a positive effect on rumen fermentation, nor in enteric methane production in crossbred heifers fed a basal ration of tropical grass.
Losing the Battle but Winning the War: Can Defeated Antibacterials Form Alliances to Combat Drug-Resistant Pathogens?
Authors: Oh Song , Chau Raymond , Nguyen Anh T. , Lenhard Justin R.
Published: May 2021

Despite the recent development of antibacterials that are active against multidrug-resistant pathogens, drug combinations are often necessary to optimize the killing of difficult-to-treat organisms. Antimicrobial combinations typically are composed of multiple agents that are active against the target organism; however, many studies have investigated the potential utility of combinations that consist of one or more antibacterials that individually are incapable of killing the relevant pathogen. The current review summarizes in vitro, in vivo, and clinical studies that evaluate combinations that include at least one drug that is not active individually against Pseudomonas aeruginosa, Klebsiella pneumoniae, Acinetobacter baumannii, or Staphylococcus aureus. Polymyxins were often included in combinations against all three of the Gram-negative pathogens, and carbapenems were commonly incorporated into combinations against K. pneumoniae and A. baumannii. Minocycline, sulbactam, and rifampin were also frequently investigated in combinations against A. baumannii, whereas the addition of ceftaroline or another β-lactam to vancomycin or daptomycin showed promise against S. aureus with reduced susceptibility to vancomycin or daptomycin. Although additional clinical studies are needed to define the optimal combination against specific drug-resistant pathogens, the large amount of in vitro and in vivo studies available in the literature may provide some guidance on the rational design of antibacterial combinations.
Spices, Condiments, Extra Virgin Olive Oil and Aromas as Not Only Flavorings, but Precious Allies for Our Wellbeing
Authors: Dini Irene , Laneri Sonia
Published: May 2021

Spices, condiments and extra virgin olive oil (EVOO) are crucial components of human history and nutrition. They are substances added to foods to improve flavor and taste. Many of them are used not only to flavor foods, but also in traditional medicine and cosmetics. They have antioxidant, antiviral, antibiotic, anticoagulant and antiinflammatory properties and exciting potential for preventing chronic degenerative diseases such as cardiomyopathy and cancer when used in the daily diet. Research and development in this particular field are deeply rooted as the consumer inclination towards natural products is significant. It is essential to let consumers know the beneficial effects of the daily consumption of spices, condiments and extra virgin olive oil so that they can choose them based on effects proven by scientific works and not by the mere illusion that plant products are suitable only because they are natural and not chemicals. The study begins with the definition of spices, condiments and extra virgin olive oil. It continues by describing the pathologies that can be prevented with a spicy diet and it concludes by considering the molecules responsible for the beneficial effects on human health (phytochemical) and their eventual transformation when cooked.
Benefits of Exercise and Astaxanthin Supplementation: Are There Additive or Synergistic Effects?
Authors: Oharomari Leandro Kansuke , Ikemoto Mitsushi J. , Hwang Dong Joo , Koizumi Hikaru , Soya Hideaki
Published: May 2021

A healthy lifestyle is essential for maintaining physical and mental health. Health promotion, with a particular emphasis on regular exercise and a healthy diet, is one of the emerging trends in healthcare. However, the way in which exercise training and nutrients from dietary intake interact with each other to promote additive, synergistic, or antagonistic effects on physiological functions leading to health promotion, and the possible underlying biomolecular mechanisms of such interactions, remain poorly understood. A healthy diet is characterized by a high intake of various bioactive compounds usually found in natural, organic, and fresh foodstuffs. Among these bioactive compounds, astaxanthin (ASX), a red carotenoid pigment especially found in seafood, has been recognized in the scientific literature as a potential nutraceutical due to its antioxidant, anti-inflammatory, and neurotrophic properties. Therefore, scientists are currently exploring whether this promising nutrient can increase the well-known benefits of exercise on health and disease prevention. Hence, the present review aimed to compile and summarize the current scientific evidence for ASX supplementation in association with exercise regimes, and evaluate the additive or synergistic effects on physiological functions and health when both interventions are combined. The new insights into the combination paradigm of exercise and nutritional supplementation raise awareness of the importance of integrative studies, particularly for future research directions in the field of health and sports nutrition science.
Effects of Folic Acid Supplementation on Oxidative Stress Markers: A Systematic Review and Meta-Analysis of Randomized Controlled Trials
Authors: Asbaghi Omid , Ghanavati Matin , Ashtary-Larky Damoon , Bagheri Reza , Rezaei Kelishadi Mahnaz , Nazarian Behzad , Nordvall Michael , Wong Alexei , Dutheil Frédéric , Suzuki Katsuhiko , Alavi Naeini Amirmansour
Published: May 2021

(1) Background: This systematic review and meta-analysis aimed to assess the effects of folic acid supplementation on oxidative stress markers. (2) Methods: Online database including PubMed, Scopus, Web of Science, and Cochrane were searched up to January 2021, to retrieve randomized controlled trials (RCTs) which examined the effect of folic acid supplementation on markers of oxidative stress. Meta-analyses were carried out using a random-effects model. I2 index was used to evaluate the heterogeneity of RCTs. (3) Results: Among the initial 2322 studies that were identified from electronic databases search, 13 studies involving 1013 participants were eligible. Pooled effect size from 13 studies indicated that folic acid supplementation elicits a significant rise in serum concentrations of glutathione (GSH) (WMD: 219.01 umol/L, 95% CI 59.30 to 378.71, p = 0.007) and total antioxidant capacity (TAC) (WMD: 91.70 umol/L, 95% CI 40.52 to 142.88, p < 0.001) but has no effect on serum concentrations of nitric oxide (NO) (WMD: 2.61 umol/L, 95% CI −3.48 to 8.72, p = 0.400). In addition, folic acid supplementation significantly reduced serum concentrations of malondialdehyde (MDA) (WMD: −0.13 umol/L, 95% CI −0.24 to −0.02, p = 0.020). (4) Conclusions: This meta-analysis study suggests that folic acid supplementation may significantly improve markers within the antioxidative defense system by increasing serum concentrations of GSH and TAC and decreasing serum concentrations of MDA.
Investigation of Stray Radiation Suppression in Infrared Imaging System Using a Novel Broadband and High-Absorption Ceramic Coating
Authors: Yang Zhen , Guo Xinmin , Lu Songtao , Zhang Yong , Hu Haili , Lu Kaichang , Zhang Jianlong
Published: May 2021

Aiming at addressing the vulnerability of the infrared imaging opto-mechanical system to stray radiation interference caused by an external laser, a new method with a broadband and high-absorptivity ceramic coating on the inner wall of the system is proposed to reduce the stray radiation intensity, which is helpful to improve the imaging quality of the detection image. Based on plasma electrolytic oxidation (PEO) technology, the preparation method and properties of a novel ceramic coating are studied, and a long-wave infrared imaging optical system with high-absorption ceramic coating is designed and developed. It is verified for the first time that the high-absorption ceramic coating can suppress the stray radiation in the infrared opto-mechanical system, and the effect of laser incident power and angle on the stray radiation is investigated. The experimental results show that the ceramic coating can achieve 95.4% high absorption in the wavelength range of 0.2–16 μm, which can obviously suppress the stray radiation in the opto-mechanical system of infrared imaging caused by laser. The calculation of the stray radiation suppression ratio shows that, compared with the case without coating, the stray radiation intensity in the system can be reduced by 70% using the ceramic coating.
Determination of Workspaces and Intersections of Robot Links in a Multi-Robotic System for Trajectory Planning
Authors: Behera Laxmidhar , Rybak Larisa , Malyshev Dmitry , Gaponenko Elena
Published: May 2021

One of the problems in the development of multi-robotic systems is the safe navigation of a group of robots. To solve it, the restrictions imposed by the structural elements of its agents are determined. The article presents a multi-robotic system consisting of parallel and serial robots installed on mobile platforms. The parallel robot is made based on a tripod with the ability to rotate the robot’s base relative to the horizontal axis. The analysis of its working and technological area is carried out, taking into account singularity zones. The developed algorithms for determining the workspaces are based on deterministic methods for approximating the set of solutions to systems of nonlinear inequalities. In this case, restrictions in spaces of different coordinates are presented in the form of n-dimensional boxes. Approaches to solving two problems are proposed to determine the possible intersection of links for the collaborative performance of tasks by a multi-robotic system. The first task is to determine the intersection of the links for the given positions and the relative position of the manipulators. The second is in determining the minimum distance between the technological areas of manipulators, which consist of the workspace and all possible positions of the intermediate links.
Thermodynamics of Soil Microbial Metabolism: Applications and Functions
Authors: Barros Nieves
Published: May 2021

The thermodynamic characterization of soils would help to study and to understand their strategies for survival, as well as defining their evolutionary state. It is still a challenging goal due to difficulties in calculating the thermodynamic state variables (enthalpy, Gibbs energy, and entropy) of the reactions taking place in, and by, soils. Advances in instrumentation and methodologies are bringing options for those calculations, boosting the interest in this subject. The thermodynamic state variables involve considering the soil microbial functions as key channels controlling the interchange of matter and energy between soil and the environment, through the concept of microbial energy use efficiency. The role of microbial diversity using the energy from the soil organic substrates, and, therefore, the who, where, with whom, and why of managing that energy is still unexplored. It could be achieved by unraveling the nature of the soil organic substrates and by monitoring the energy released by the soil microbial metabolism when decomposing and assimilating those substrates. This review shows the state of the art of these concepts and the future impact of thermodynamics on soil science and on soil ecology.