bluuebunny/crossref_metadata_2025 huggingface dataset:
Characterizing ingestive behavior through licking microstructure: Underlying neurobiology and its use in the study of obesity in animal models
Authors: Johnson Alexander W.
Published: July 2017

Ingestive behavior is controlled by multiple distinct peripheral and central physiological mechanisms that ultimately determine whether a particular food should be accepted or avoided. As rodents consume a fluid they display stereotyped rhythmic tongue movements, and by analyzing the temporal distribution of pauses of licking, it is possible through analyses of licking microstructure to uncover dissociable evaluative and motivational variables that contribute to ingestive behavior. The mean number of licks occurring within each burst of licking (burst and cluster size) reflects the palatability of the consumed solution, whereas the frequency of initiating novel bouts of licking behavior (burst and cluster number) is dependent upon the degree of gastrointestinal inhibition that accrues through continued fluid ingestion. This review describes the analysis of these measures within a context of the behavioral variables that come to influence the acceptance or avoidance of a fluid, and the neurobiological mechanisms that underlie alterations in the temporal distribution of pauses of licks. The application of these studies to models of obesity in animals is also described.
Second Person Pronouns Enhance Consumer Involvement and Brand Attitude
Authors: Cruz Ryan E. , Leonhardt James M. , Pezzuti Todd
Published: July 2017

Online brand messaging, e.g., blogging or posting on social media platforms, has an important role in digital marketing strategy. Such messaging is largely text based and provides an opportunity for brands to interact with many consumers simultaneously. The marketing literature, however, has yet to provide sufficient guidance on effective online brand messaging strategies. In particular, research has yet to address how the inclusion of second person pronouns in online brand messaging affects relevant consumer outcomes. The present research proposes that second person pronouns should work to enhance consumer involvement and brand attitude as a result of increasing the extent that consumers engage in self-referencing. A field study involving actual brand posts on Facebook and two subsequent experiments provide support for this hypothesis. In addition, drawing on cultural dimensions theory, individual levels of collectivism are identified as a boundary condition. The presence (vs. absence) of second person pronouns in online brand messaging enhances involvement and brand attitude for consumers that are lower, but not higher, in collectivism. The results provide marketers with needed guidance for creating effective online brand messaging.
Cervical Spine Alignment in Helmeted Skiers and Snowboarders With Suspected Head and Neck Injuries: Comparison of Lateral C-spine Radiographs Before and After Helmet Removal and Implications for Ski Patrol Transport
Authors: Murray Jared , Rust David A.
Published: July 2017

Objective

Current protocols for spine immobilization of the injured skier/snowboarder have not been scientifically validated. Observing changes in spine alignment during common rescue scenarios will help strengthen recommendations for rescue guidelines.

Methods

Twenty-eight healthy volunteers (18 men, 10 women) age 47±17 (range 20–73) (mean ±SD with range) underwent a mock rescue in which candidate patrollers completing an Outdoor Emergency Care course performed spine immobilization and back boarding in 3 scenarios: 1) Ski helmet on, no c-collar; 2) helmet on, with c-collar; and 3) helmet removed, with c-collar. After each scenario, a lateral radiograph was taken of the cervical spine to observe for changes in alignment.

Results

Compared with the control group (helmet on, no collar), we observed 9 degrees of increased overall (occiput–C7) cervical extension in the helmet on, with collar group ( P < .001), and 17 degrees in the helmet off, with collar group ( P < .001). There was increased extension at the occiput–C2 intersegment in the helmet on, with collar group (9 degrees, P < .001) and at both the occiput–C2 (9 degrees, P < .001) and C2–C7 (8 degrees, P < .001) intersegments in the helmet off, with collar group.

Conclusion

Ski helmet removal and c-collar application each leads to increased extension of the cervical spine. In the absence of other clinical factors, our recommendation is that helmets should be left in place and c-collars not routinely applied during ski patrol rescue.
Country-of-Origin Labelling, Food Traceability Drivers and Food Fraud: Lessons from Consumers’ Preferences and Perceptions
Authors: BITZIOS Michail , JACK Lisa , KRZYZANIAK Sally-Ann , XU Mark
Published: July 2017

Many factors influence consumers’ perceptions and purchasing decisions, with product labelling forming the primary means of communication. The extent to which labels should contain information about traceability is debated. Whilst traceability is an important tool used by food business organisations and regulators in assuring food safety, other drivers for information about traceability are less well understood. This paper reviews the issues related to drivers for traceability from a consumer perspective, and evaluates country-of-origin labelling (COOL), enabling technologies and food fraud as potentially significant drivers in consumer requirements for information. The implications for risk assessment, systems implementation and communications about traceability are also considered.
Regulatory Sanctions and Reputational Damage in Financial Markets
Authors: Armour John , Mayer Colin , Polo Andrea
Published: July 2017

We study the impact of the enforcement of financial regulation by the United Kingdom’s regulatory authorities on the market price of penalized firms. Existing studies rely on analyses of multiple events that may distort the measurement of reputational losses. In the United Kingdom, the entire enforcement process involves only one public announcement and is accompanied by complete information on legal penalties. We find that reputational losses are nearly nine times the size of fines and are associated with misconduct harming customers or investors but not third parties.
THE CONSISTENCY FOR THE WEIGHTED ESTIMATOR OF NON-PARAMETRIC REGRESSION MODEL BASED ON WIDELY ORTHANT-DEPENDENT ERRORS
Authors: Xia Hao , Wu Yi , Tao Xinran , Wang Xuejun
Published: July 2017

In this paper, the complete consistency for the weighted estimator of non-parametric regression model based on widely orthant-dependent errors is established, where the restriction imposed on the dominating coefficient g(n) is very general. Moreover, under some stronger moment condition, we further obtain the convergence rate of the complete consistency, where the assumption on the dominating coefficient g(n) is also very general.
Administrative Reform and the Transfer of Authority to Social Organizations in China
Authors: Gao Hong , Tyson Adam
Published: July 2017

In this article, we examine the administrative functions that have been carried out by social organizations (SOs) in China since 2013. We use evidence from Guangdong to demonstrate that the transfer of authority to SOs is selective, tends to create more burdens for local government, and generally does not lead to greater autonomy for SOs. We focus on five types of SOs that are undertaking new administrative functions with varying degrees of operational autonomy, which relates to the consultative authoritarian model proposed by Jessica Teets. Consultative authoritarianism allows for the expansion of relatively autonomous SOs and the development of indirect state control mechanisms. The model is designed to improve governance without democratization by expanding the role played by intermediaries such as SOs in public administration and service delivery. The evidence from Guangdong permits us to conclude that the transfer of authority to SOs allows for innovations in public administration, but that politics continues to motivate government decisions as to which functions are suitable for SOs to undertake, casting doubt on the ability of the Chinese Communist Party to achieve sustainable improvements in local governance and public service provision.
Vessel Spatio-temporal Knowledge Discovery with AIS Trajectories Using Co-clustering
Authors: Wang Jiang , Zhu Cheng , Zhou Yun , Zhang Weiming
Published: July 2017

Large volumes of data collected by the Automatic Identification System (AIS) provide opportunities for studying both single vessel motion behaviours and collective mobility patterns on the sea. Understanding these behaviours or patterns is of great importance to maritime situational awareness applications. In this paper, we leveraged AIS trajectories to discover vessel spatio-temporal co-occurrence patterns, which distinguish vessel behaviours simultaneously in terms of space, time and other dimensions (such as ship type, speed, width etc.). To this end, available AIS data were processed to generate spatio-temporal matrices and spatio-temporal tensors (i.e., multidimensional arrays). We then imposed a sparse bilinear decomposition on the matrices and a sparse multi-linear decomposition on the tensors. Experimental results on a real-world dataset demonstrated the effectiveness of this methodology, with which we show the existence of connection among regions, time, and vessel attributes.
Morphogenesis-related gene-expression profile in porcine oocytes before and after in vitro maturation
Authors: Budna Joanna , Chachuła Adrian , Kaźmierczak Dominika , Rybska Marta , Ciesiółka Sylwia , Bryja Artur , Kranc Wiesława , Borys Sylwia , Żok Agnieszka , Bukowska Dorota , Antosik Paweł , Bruska Małgorzata , Brüssow Klaus P. , Nowicki Michał , Zabel Maciej , Kempisty Bartosz
Published: July 2017

Mammalian oocyte maturation is achieved when oocytes reach metaphase II (MII) stage, and accumulate mRNA and proteins in the cytoplasm following fertilization. It has been shown that oocytes investigated before and after in vitro maturation (IVM) differ significantly in transcriptomic and proteomic profiles. Additionally, folliculogenesis and oogenesis is accompanied by morphogenetic changes, which significantly influence further zygote formation and embryo growth. This study aimed to determine new transcriptomic markers of porcine oocyte morphogenesis that are associated with cell maturation competence. An Affymetrix microarray assay was performed on an RNA template isolated from porcine oocytes before (n = 150) and after (n = 150) IVM. The brilliant cresyl blue (BCB) staining test was used for identification of cells with the highest developmental capacity. DAVID (Database for Annotation, Visualization, and Integrated Discovery) software was used for the extraction of the genes belonging to a cell morphogenesis Gene Ontology group. The control group consisted of freshly isolated oocytes. In total, 12,000 different transcripts were analysed, from which 379 genes were downregulated and 40 were upregulated in oocytes following IVM. We found five genes, SOX9, MAP1B, DAB2, FN1, and CXCL12, that were significantly upregulated in oocytes after IVM (in vitro group) compared with oocytes analysed before IVM (in vivo group). In conclusion, we found new transcriptomic markers of oocyte morphogenesis, which may be also recognized as significant mediators of cellular maturation capacity in pigs. Genes SOX9, MAP1B, DAB2, FN1, and CXCL12 may be involved in the regulation of the MII stage oocyte formation and several other processes that are crucial for porcine reproductive competence.
Expression of markers for germ cells and oocytes in cow dermal fibroblast treated with 5-azacytidine and cultured in differentiation medium containing BMP2, BMP4 or follicular fluid
Authors: Costa José Jackson do Nascimento , de Souza Glaucinete Borges , Bernardo Joyla Maria Pires , Ribeiro Regislane Pinto , Passos José Renato de Souza , Bezerra Francisco Taiã Gomes , Saraiva Márcia Viviane Alves , Silva José Roberto Viana
Published: July 2017

This study aims to investigate the effect 5-azacytidine (5-Aza) during induction of pluripotency in bovine fibroblasts and to evaluate the effects of BMP2, BMP4 or follicular fluid in the differentiation of reprogrammed fibroblasts in primordial germ cells and oocytes. It also analysis the mRNA levels forOCT4,NANOG,REX,SOX2,VASA,DAZL,cKIT,SCP3,ZPAandGDF9after culturing 5-Aza treated fibroblasts in the different tested medium. Dermal fibroblasts were cultured and exposed to 0.5, 1.0 or 2.0 μM of 5-Aza for 18 h, 36 h or 72 h. Then, the cells were cultured in DMEM/F12 supplemented with 10 ng/ml BMP2, 10 ng/ml BMP4 or 5% follicular fluid. After culture, morphological characteristics, viability and gene expression were evaluated by qPCR. Treatment of skin fibroblasts with 2.0 μM 5-Aza for 72 h significantly increased expression of mRNAs for SOX2, OCT4, NANOG and REX. The culture in medium supplemented with BMP2, BMP4 or follicular fluid for 7 or 14 days induced formation of oocyte-like cells, as well as the expression of markers for germ cells and oocyte. In conclusion, treatment of bovine skin-derived fibroblasts with 2.0 μM 5-Aza for 72 h induces the expression of pluripotency factors. Culturing these cells in differentiation medium supplemented with BMP2, BMP4 or follicular fluid induces morphological changes and promotes expression of markers for germ cells, meiosis and oocyte.
LIMITED ASSET MARKET PARTICIPATION AND DETERMINACY IN THE OPEN ECONOMY
Authors: Buffie Edward F. , Zanna Luis-Felipe
Published: July 2017

The perception that inflation targeting (IT) runs a high risk of indeterminacy when a significant share of households are too poor to save is an artifact of the closed economy. In the open economy, the Taylor principle is generally valid for both contemporaneous and forward-looking IT. Active policy in contemporaneous IT guarantees determinacy, eccentric cases aside. In forward-looking IT, the scope for active policy is constrained by an upper bound on the Taylor coefficient. The upper bound is insensitive, however, to the share of poor, nonsaving households. Moreover, it can be increased substantially–to a level that does not bind–through reserve sales/purchases that limit exchange rate volatility.
OPTIMAL PAY-AS-YOU-GO SOCIAL SECURITY WITH ENDOGENOUS RETIREMENT
Authors: Miyazaki Koichi
Published: July 2017

This paper considers an overlapping-generations model with pay-as-you-go social security and retirement decision making by an old agent. In addition, this paper assumes that labor productivity depreciates. Under this setting, socially optimal allocations are examined. The first-best allocation is an allocation that maximizes welfare when a social planner distributes resources and forces an old agent to work and retire as she wants. The second-best allocation is one that maximizes welfare when a social planner can use only pay-as-you-go social security in a decentralized economy. This paper finds a range of an old agent's labor productivity such that the first-best allocation is achieved in a decentralized economy. This finding differs from that in Michel and Pestieau [“Social security and early retirement in an overlapping-generations growth model”, Annals of Economics & Finance, 2013], which notes that the first-best allocation cannot be achieved in a decentralized economy.
Avoiding Adversariness? The Effects of Gender on Litigant Decision-Making
Authors: Wofford Claire B.
Published: July 2017

How does gender influence the American judicial system? Scholars interested in this question have focused on several areas, including the substance of the law (Bender 1993; Olsen 1995; Rifkin 1981), the presence of women on a jury (Fowler 2005; Marder 1987; Taylor-Thompson 2000), and the sex of criminal defendants (Sarnikar, Sorenson, and Oaxaca 2007; Starr 2012). Among political scientists in particular, most work has examined judicial decision-making and whether the rulings of female judges differ from those of their male counterparts. Results of these studies have been mixed: some indicate that female judges vote differently, at least in certain types of cases (Boyd, Epstein, and Martin 2010; Peresie 2005; Songer and Crews-Meyer 2000), but others have found no, or only a minimal, gender effect (Segal 2002; Songer, Davis, and Haire 1994; Walker and Barrow 1985).
Detecting cell-secreted growth factors in microfluidic devices using bead-based biosensors
Authors: Son Kyung Jin , Gheibi Pantea , Stybayeva Gulnaz , Rahimian Ali , Revzin Alexander
Published: July 2017

Microfluidic systems provide an interesting alternative to standard macroscale cell cultures due to the decrease in the number of cells and reagents as well as the improved physiology of cells confined to small volumes. However, the tools available for cell-secreted molecules inside microfluidic devices remain limited. In this paper, we describe an integrated microsystem composed of a microfluidic device and a fluorescent microbead-based assay for the detection of the hepatocyte growth factor (HGF) and the transforming growth factor (TGF)-β1 secreted by primary hepatocytes. This microfluidic system is designed to separate a cell culture chamber from sensing chambers using a permeable hydrogel barrier. Cell-secreted HGF and TGF-β1 diffuse through the hydrogel barrier into adjacent sensing channels and are detected using fluorescent microbead-based sensors. The specificity of sensing microbeads is defined by the choice of antibodies; therefore, our microfluidic culture system and sensing microbeads may be applied to a variety of cells and cell-secreted factors.
Transplantation of enteric nervous system stem cells rescues nitric oxide synthase deficient mouse colon
Authors: McCann Conor J. , Cooper Julie E. , Natarajan Dipa , Jevans Benjamin , Burnett Laura E. , Burns Alan J. , Thapar Nikhil
Published: July 2017

Enteric nervous system neuropathy causes a wide range of severe gut motility disorders. Cell replacement of lost neurons using enteric neural stem cells (ENSC) is a possible therapy for these life-limiting disorders. Here we show rescue of gut motility after ENSC transplantation in a mouse model of human enteric neuropathy, the neuronal nitric oxide synthase (nNOS−/−) deficient mouse model, which displays slow transit in the colon. We further show that transplantation of ENSC into the colon rescues impaired colonic motility with formation of extensive networks of transplanted cells, including the development of nNOS+neurons and subsequent restoration of nitrergic responses. Moreover, post-transplantation non-cell-autonomous mechanisms restore the numbers of interstitial cells of Cajal that are reduced in thenNOS−/−colon. These results provide the first direct evidence that ENSC transplantation can modulate the enteric neuromuscular syncytium to restore function, at the organ level, in a dysmotile gastrointestinal disease model.
The A-B transition in superfluid helium-3 under confinement in a thin slab geometry
Authors: Zhelev N. , Abhilash T. S. , Smith E. N. , Bennett R. G. , Rojas X. , Levitin L. , Saunders J. , Parpia J. M.
Published: July 2017

The influence of confinement on the phases of superfluid helium-3 is studied using the torsional pendulum method. We focus on the transition between the A and B phases, where the A phase is stabilized by confinement and a spatially modulated stripe phase is predicted at the A–B phase boundary. Here we discuss results from superfluid helium-3 contained in a single 1.08-μm-thick nanofluidic cavity incorporated into a high-precision torsion pendulum, and map the phase diagram between 0.1 and 5.6 bar. We observe only small supercooling of the A phase, in comparison to bulk or when confined in aerogel, with evidence for a non-monotonic pressure dependence. This suggests that an intrinsic B-phase nucleation mechanism operates under confinement. Both the phase diagram and the relative superfluid fraction of the A and B phases, show that strong coupling is present at all pressures, with implications for the stability of the stripe phase.
JWA regulates TRAIL-induced apoptosis via MARCH8-mediated DR4 ubiquitination in cisplatin-resistant gastric cancer cells
Authors: Wang Q , Chen Q , Zhu L , Chen M , Xu W , Panday S , Wang Z , Li A , Røe O D , Chen R , Wang S , Zhang R , Zhou J
Published: July 2017

Platinum chemotherapeutics are widely used to treat solid malignant tumors, including gastric cancer (GC). Drug resistance to platinum compounds may result in cancer relapse and decreased survival. The identification and development of novel agents to reactivate apoptosis pathways in platinum-resistant cancer cells is therefore necessary. Here we report that cisplatin-resistant human GC cells (BGC823/DDP and SGC7901/DDP) but not their parental cells (BGC823 and SGC7901) exhibit high sensitivity to tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) as a result of overexpression of death receptor 4 (DR4). Furthermore, we found that JWA, a molecule that promotes cisplatin-induced apoptosis in GC cells, suppressed TRAIL-induced apoptosis via negative regulation of DR4. Mechanistically, JWA promoted the ubiquitination of DR4 at K273 via upregulation of the ubiquitin ligase membrane-associated RING-CH-8 (MARCH8). In human GC tissues, JWA and DR4 protein levels were negatively correlated. Thus TRAIL may serve as an auxiliary treatment for cisplatin-resistant GC, and JWA may be a potential predictive marker of TRAIL sensitivity and may improve personalized therapeutics for treating human GC.
Human snoRNA-93 is processed into a microRNA-like RNA that promotes breast cancer cell invasion
Authors: Patterson Dillon G. , Roberts Justin T. , King Valeria M. , Houserova Dominika , Barnhill Emmaline C. , Crucello Aline , Polska Caroline J. , Brantley Lucas W. , Kaufman Garrett C. , Nguyen Michael , Santana Megann W. , Schiller Ian A. , Spicciani Julius S. , Zapata Anastasia K. , Miller Molly M. , Sherman Timothy D. , Ma Ruixia , Zhao Hongyou , Arora Ritu , Coley Alexander B. , Zeidan Melody M. , Tan Ming , Xi Yaguang , Borchert Glen M.
Published: July 2017

Genetic searches for tumor suppressors have recently linked small nucleolar RNA misregulations with tumorigenesis. In addition to their classically defined functions, several small nucleolar RNAs are now known to be processed into short microRNA-like fragments called small nucleolar RNA-derived RNAs. To determine if any small nucleolar RNA-derived RNAs contribute to breast malignancy, we recently performed a RNA-seq-based comparison of the small nucleolar RNA-derived RNAs of two breast cancer cell lines (MCF-7 and MDA-MB-231) and identified small nucleolar RNA-derived RNAs derived from 13 small nucleolar RNAs overexpressed in MDA-MB-231s. Importantly, we find that inhibiting the most differentially expressed of these small nucleolar RNA-derived RNAs (sdRNA-93) in MDA-MB-231 cells results primarily in a loss of invasiveness, whereas increased sdRNA-93 expression in either cell line conversely results in strikingly enhanced invasion. Excitingly, we recently determined sdRNA-93 expressions in small RNA-seq data corresponding to 116 patient tumors and normal breast controls, and while we find little sdRNA-93 expression in any of the controls and only sporadic expression in most subtypes, we find robust expression of sdRNA-93 in 92.8% of Luminal B Her2+tumors. Of note, our analyses also indicate that at least one of sdRNA-93’s endogenous roles is to regulate the expression of Pipox, a sarcosine metabolism-related protein whose expression significantly correlates with distinct molecular subtypes of breast cancer. We find sdRNA-93 can regulate the Pipox 3′UTR via standard reporter assays and that manipulating endogenous sdRNA-93 levels inversely correlates with altered Pipox expression. In summary, our results strongly indicate that sdRNA-93 expression actively contributes to the malignant phenotype of breast cancer through participating in microRNA-like regulation.
A homozygous mutation in the stem II domain of RNU4ATAC causes typical Roifman syndrome
Authors: Dinur Schejter Yael , Ovadia Adi , Alexandrova Roumiana , Thiruvahindrapuram Bhooma , Pereira Sergio L. , Manson David E. , Vincent Ajoy , Merico Daniele , Roifman Chaim M.
Published: July 2017

Roifman syndrome (OMIM# 616651) is a complex syndrome encompassing skeletal dysplasia, immunodeficiency, retinal dystrophy and developmental delay, and is caused by compound heterozygous mutations involving the Stem II region and one of the other domains of the RNU4ATAC gene. This small nuclear RNA gene is essential for minor intron splicing. The Canadian Centre for Primary Immunodeficiency Registry and Repository were used to derive patient information as well as tissues. Utilising RNA sequencing methodologies, we analysed samples from patients with Roifman syndrome and assessed intron retention. We demonstrate that a homozygous mutation in Stem II is sufficient to cause the full spectrum of features associated with typical Roifman syndrome. Further, we demonstrate the same pattern of aberration in minor intron retention as found in cases with compound heterozygous mutations.
Response inhibition in Parkinson’s disease: a meta-analysis of dopaminergic medication and disease duration effects
Authors: Manza Peter , Amandola Matthew , Tatineni Vivekanand , Li Chiang-shan R. , Leung Hoi-Chung
Published: July 2017

Parkinson’s disease is a neurodegenerative disorder involving the basal ganglia that results in a host of motor and cognitive deficits. Dopamine-replacement therapy ameliorates some of the hallmark motor symptoms of Parkinson’s disease, but whether these medications improve deficits in response inhibition, a critical executive function for behavioral control, has been questioned. Several studies of Parkinson’s disease patients “on” and “off” (12-h withdrawal) dopaminergic medications suggested that dopamine-replacement therapy did not provide significant response inhibition benefits. However, these studies tended to include patients with moderate-to-advanced Parkinson’s disease, when the efficacy of dopaminergic drugs is reduced compared to early-stage Parkinson’s disease. In contrast, a few recent studies in early-stage Parkinson’s disease report that dopaminergic drugs do improve response inhibition deficits. Based on these findings, we hypothesized that Parkinson’s disease duration interacts with medication status to produce changes in cognitive function. To investigate this issue, we conducted a meta-analysis of studies comparing patients with Parkinson’s disease and healthy controls on tests of response inhibition (50 comparisons from 42 studies). The findings supported the hypothesis; medication benefited response inhibition in patients with shorter disease duration, whereas “off” medication, moderate deficits were present that were relatively unaffected by disease duration. These findings support the role of dopamine in response inhibition and suggest the need to consider disease duration in research of the efficacy of dopamine-replacement therapy on cognitive function in Parkinson’s disease.
Gasoline cars produce more carbonaceous particulate matter than modern filter-equipped diesel cars
Authors: Platt S. M. , El Haddad I. , Pieber S. M. , Zardini A. A. , Suarez-Bertoa R. , Clairotte M. , Daellenbach K. R. , Huang R.-J. , Slowik J. G. , Hellebust S. , Temime-Roussel B. , Marchand N. , de Gouw J. , Jimenez J. L. , Hayes P. L. , Robinson A. L. , Baltensperger U. , Astorga C. , Prévôt A. S. H.
Published: July 2017

Carbonaceous particulate matter (PM), comprising black carbon (BC), primary organic aerosol (POA) and secondary organic aerosol (SOA, from atmospheric aging of precursors), is a highly toxic vehicle exhaust component. Therefore, understanding vehicle pollution requires knowledge of both primary emissions, and how these emissions age in the atmosphere. We provide a systematic examination of carbonaceous PM emissions and parameterisation of SOA formation from modern diesel and gasoline cars at different temperatures (22, −7 °C) during controlled laboratory experiments. Carbonaceous PM emission and SOA formation is markedly higher from gasoline than diesel particle filter (DPF) and catalyst-equipped diesel cars, more so at −7 °C, contrasting with nitrogen oxides (NOX). Higher SOA formation from gasoline cars and primary emission reductions for diesels implies gasoline cars will increasingly dominate vehicular total carbonaceous PM, though older non-DPF-equipped diesels will continue to dominate the primary fraction for some time. Supported by state-of-the-art source apportionment of ambient fossil fuel derived PM, our results show that whether gasoline or diesel cars are more polluting depends on the pollutant in question, i.e. that diesel cars are not necessarily worse polluters than gasoline cars.
Genetic variants of TRAF6 modulate peritoneal immunity and the risk of spontaneous bacterial peritonitis in cirrhosis: A combined prospective-retrospective study
Authors: Mai Martina , Stengel Sven , Al-Herwi Eihab , Peter Jack , Schmidt Caroline , Rubio Ignacio , Stallmach Andreas , Bruns Tony
Published: July 2017

Alterations of the innate immunity contribute to the development of spontaneous bacterial peritonitis (SBP) in liver cirrhosis. Given its role in immune signaling, antimicrobial function, and macrophage differentiation, we hypothesized that genetic polymorphisms of TRAF6 modulate the risk of SBP. Thus, we determined theTRAF6 haplotype in 432 patients with cirrhosis and ascites using the haplotype-tagging single nucleotide polymorphisms rs331457 and rs5030419. In addition, peritoneal macrophages were immunomagnetically isolated and characterized. Overall, 122 (28%) patients had an episode of SBP. In the combined prospective-retrospective analysis the frequency of SBP differed between the four haplotypes (P = 0.014) and was the highest in 102 patients carrying the rs331457 but not the rs5030419 variant, when compared to other haplotypes (odds ratio 1.95 [1.22–3.12]) or to the wild-type (odds ratio 1.71 [1.04–2.82]). This association was confirmed in multivariate logistic regression (adjusted odds ratio 2.00 [1.24–3.22]) and in prospective sensitivity analysis (hazard ratio 2.09 [1.08–4.07]; P = 0.03). The risk haplotype was associated with lower concentrations of the immune activation marker soluble CD87 in ascitic fluid and with a decreased expression of IL-6 and CXCL8 in isolated peritoneal macrophages. In conclusion, genetic polymorphisms of TRAF6 are associated with decreased peritoneal immune activation and an increased risk of SBP.
Blast-Induced Tinnitus and Elevated Central Auditory and Limbic Activity in Rats: A Manganese-Enhanced MRI and Behavioral Study
Authors: Ouyang Jessica , Pace Edward , Lepczyk Laura , Kaufman Michael , Zhang Jessica , Perrine Shane A. , Zhang Jinsheng
Published: July 2017

Blast-induced tinitus is the number one service-connected disability that currently affects military personnel and veterans. To elucidate its underlying mechanisms, we subjected 13 Sprague Dawley adult rats to unilateral 14 psi blast exposure to induce tinnitus and measured auditory and limbic brain activity using manganese-enhanced MRI (MEMRI). Tinnitus was evaluated with a gap detection acoustic startle reflex paradigm, while hearing status was assessed with prepulse inhibition (PPI) and auditory brainstem responses (﻿ABR﻿﻿s). Both anxiety and cognitive functioning were assessed using elevated plus maze and Morris water maze, respectively. Five weeks after blast exposure, 8 of the 13 blasted rats exhibited chronic tinnitus. While acoustic PPI remained intact and ABR thresholds recovered, the ABR wave P1-N1 amplitude reduction persisted in all blast-exposed rats. No differences in spatial cognition were observed, but blasted rats as a whole exhibited increased anxiety. MEMRI data revealed a bilateral increase in activity along the auditory pathway and in certain limbic regions of rats with tinnitus compared to age-matched controls. Taken together, our data suggest that while blast-induced tinnitus may play a role in auditory and limbic hyperactivity, the non-auditory effects of blast and potential traumatic brain injury may also exert an effect.
Barbier-type anti-Diastereo- and Enantioselective Synthesis of β-Trimethylsilyl, Fluorinated Methyl, Phenylthio Homoallylic Alcohols
Authors: Guo Rui , Yang Qin , Tian Qinshan , Zhang Guozhu
Published: July 2017

Catalytic Asymmetric allylation of aldehydes with functionalized allylic reagents represents an important process in synthetic organic chemistry because the resulting chiral homoallylic alcohols are valuable building blocks in diverse research fields. Despite the obvious advantages of allyl halides as allylation reagent under Barbier-type conditions, catalytic asymmetric version using functionalized allyl halides remains largely underdeveloped. Here, we addressed this issue by employing a chromium-catalysis system. The use of readily available allyl bromides with γ substitutions including trimethylsilyl, fluorinated methyl and phenylthio groups provided an efficient and convenient method to introduce those privileged functionalities into homoallylic alcohols. Good yields, high anti-diastereo- and excellent enantioselectivities were achieved under mild reaction conditions.
Achieving low-emissivity materials with high transmission for broadband radio-frequency signals
Authors: Liu Liu , Chang Huiting , Xu Tao , Song Yanan , Zhang Chi , Hang Zhi Hong , Hu Xinhua
Published: July 2017

The use of low-emissivity (low-e) materials in modern buildings is an extremely efficient way to save energy. However, such materials are coated by metallic films, which can strongly block radio-frequency signals and prevent indoor-outdoor wireless communication. Here, we demonstrate that, when specially-designed metallic metasurfaces are covered on them, the low-e materials can remain low emissivity for thermal radiation and allow very high transmission for a broad band of radio-frequency signals. It is found that the application of air-connected metasurfaces with subwavelength periods is critical to the observed high transmission. Such effects disappear if periods are comparable to wavelengths or metal-connected structures are utilized. The conclusion is supported by both simulations and experiments. Advantages such as easy to process, low cost, large-area fabrication and design versatility of the metasurface make it a promising candidate to solve the indoor outdoor communication problem.
Tunable magnetization and damping of sputter-deposited, exchange coupled Py|Fe bilayers
Authors: Omelchenko Pavlo , Montoya Eric Arturo , Coutts Chris , Heinrich Bret , Girt Erol
Published: July 2017

We report on magnetic damping of exchange coupled, polycrystalline Py(Ni80Fe20)|Fe and Fe|Py bilayers, prepared by sputter-deposition on an amorphous 3 nm Ta seed layer. FMR measurements are performed on varying thicknesses of the individual Py and Fe layers while keeping the total bilayer structure thickness fixed. When Fe is grown directly on Ta, there is large magnetic inhomogeneity and damping. However, when a Py layer is deposited between Fe and Ta, both the magnetic inhomogeneity and damping significantly decrease even if Fe is covered by Ta. The intrinsic damping of the Ta|Py|Fe film can be further lowered by increasing the Fe to Py ratio. SQUID measurements show a linear increase in saturation magnetization with increasing ratio of Fe to Py. A combination of in-plane and out-of-plane X-ray diffraction measurements show that Py is textured along the 〈111〉 directions and Fe is textured along the 〈110〉, with Fe texture significantly improving if it is deposited on Ta|Py instead of Ta. By improving the texture of Fe by introducing a thin Py layer between Fe and Ta, one can grow Fe thin films with zero in-plane anisotropy, tunable magnetic moment, and low magnetic damping, approaching that of the best single crystal Fe.
Network Structure Explains the Impact of Attitudes on Voting Decisions
Authors: Dalege Jonas , Borsboom Denny , van Harreveld Frenk , Waldorp Lourens J. , van der Maas Han L. J.
Published: July 2017

Attitudes can have a profound impact on socially relevant behaviours, such as voting. However, this effect is not uniform across situations or individuals, and it is at present difficult to predict whether attitudes will predict behaviour in any given circumstance. Using a network model, we demonstrate that (a) more strongly connected attitude networks have a stronger impact on behaviour, and (b) within any given attitude network, the most central attitude elements have the strongest impact. We test these hypotheses using data on voting and attitudes toward presidential candidates in the US presidential elections from 1980 to 2012. These analyses confirm that the predictive value of attitude networks depends almost entirely on their level of connectivity, with more central attitude elements having stronger impact. The impact of attitudes on voting behaviour can thus be reliably determined before elections take place by using network analyses.
A possible origin population of pathogenic intestinal nematodes, Strongyloides stercoralis, unveiled by molecular phylogeny
Authors: Nagayasu Eiji , Aung Myo Pa Pa Thet Hnin Htwe , Hortiwakul Thanaporn , Hino Akina , Tanaka Teruhisa , Higashiarakawa Miwa , Olia Alex , Taniguchi Tomoyo , Win Soe Moe Thu , Ohashi Isao , Odongo-Aginya Emmanuel Igwaro , Aye Khin Myo , Mon Mon , Win Kyu Kyu , Ota Kei , Torisu Yukari , Panthuwong Siripen , Kimura Eisaku , Palacpac Nirianne M. Q. , Kikuchi Taisei , Hirata Tetsuo , Torisu Shidow , Hisaeda Hajime , Horii Toshihiro , Fujita Jiro , Htike Wah Win , Maruyama Haruhiko
Published: July 2017

Humans and dogs are the two major hosts of Strongyloides stercoralis, an intestinal parasitic nematode. To better understand the phylogenetic relationships among S. stercoralis isolates infecting humans and dogs and to assess the zoonotic potential of this parasite, we analyzed mitochondrial Cox1, nuclear 18S rDNA, 28S rDNA, and a major sperm protein domain-containing protein genes. Overall, our analyses indicated the presence of two distinct lineages of S. stercoralis (referred to as type A and type B). While type A parasites were isolated both from humans and dogs in different countries, type B parasites were found exclusively in dogs, indicating that the type B has not adapted to infect humans. These epidemiological data, together with the close phylogenetic relationship of S. stercoralis with S. procyonis, a Strongyloides parasite of raccoons, possibly indicates that S. stercoralis originally evolved as a canid parasite, and later spread into humans. The inability to infect humans might be an ancestral character of this species and the type B might be surmised to be an origin population from which human-infecting strains are derived.
Long-range Stripe Nanodomains in Epitaxial (110) BiFeO3 Thin Films on (100) NdGaO3 Substrate
Authors: Sharma Yogesh , Agarwal Radhe , Phatak Charudatta , Kim Bumsoo , Jeon Seokwoo , Katiyar Ram S. , Hong Seungbum
Published: July 2017

Here, we report the observation of ferroelectric and ferroelastic nanodomains in (110)-oriented BiFeO3 (BFO) thin films epitaxially grown on low symmetric (100) NdGaO3 (NGO) substrate. We observed long range ordering of ferroelectric 109° stripe nanodomains separated by periodic vertical domain walls in as-grown 130 nm thick BFO films. The effect of La0.67Sr0.33CoO3 (LSCO) conducting interlayer on domain configurations in BFO/NGO film was also observed with relatively short range-ordering of stripe domains due to the modified electrostatic boundary conditions in BFO/LSCO/NGO film. Additional studies on B-site doping of Nb ions in BFO films showed change in the domain structures due to doping induced change in lattice anisotropy while maintaining the stripe domain morphology with 109° domain wall. This long-range array of ferroelectric and ferroelastic domains can be useful for optoelectronic devices and ferroelastic templates for strain coupled artificial magnetoelectric heterostructures.
On the improved electrochemistry of hybrid conducting-redox polymer electrodes
Authors: Sieuw Louis , Ernould Bruno , Gohy Jean-François , Vlad Alexandru
Published: July 2017

The electrochemistry of poly(2,5-dihydroxyaniline) (PDHA), a novel hybrid molecular configuration with redox active sites and electrical charge conduction along the polymer chain, has been recently reported. The theoretical capacity of this material is estimated at 443 mAh g−1, with high power performances being proposed given the intrinsic electrical conductivity. However, the initial results were below the expectations: only half the theoretical capacity attained, poor cycling stability and modest power behavior calling for further investigations on improving these performances. Herein we detail the optimized chemical synthesis and electrode formulation for poly(2,5-dihydroxyaniline) resulting in improved cycling stability, power performances and defined electrochemical response. We also detail the alternative electrochemical synthesis and activation route for PDHA and compare the results with the chemical approach.
Alpha 2 Na+,K+-ATPase silencing induces loss of inflammatory response and ouabain protection in glial cells
Authors: Kinoshita Paula F. , Yshii Lidia M. , Orellana Ana Maria M. , Paixão Amanda G. , Vasconcelos Andrea R. , Lima Larissa de Sá , Kawamoto Elisa M. , Scavone Cristoforo
Published: July 2017

Ouabain (OUA) is a cardiac glycoside that binds to Na+,K+-ATPase (NKA), a conserved membrane protein that controls cell transmembrane ionic concentrations and requires ATP hydrolysis. At nM concentrations, OUA activates signaling pathways that are not related to its typical inhibitory effect on the NKA pump. Activation of these signaling pathways protects against some types of injury of the kidneys and central nervous system. There are 4 isoforms of the alpha subunit of NKA, which are differentially distributed across tissues and may have different physiological roles. Glial cells are important regulators of injury and inflammation in the brain and express the α1 and α2 NKA isoforms. This study investigated the role of α2 NKA in OUA modulation of the neuroinflammatory response induced by lipopolysaccharide (LPS) in mouse primary glial cell cultures. LPS treatment increased lactate dehydrogenase release, while OUA did not decrease cell viability and blocked LPS-induced NF-κB activation. Silencing α2 NKA prevented ERK and NF-κB activation by LPS. α2 NKA also regulates TNF-α and IL-1β levels. The data reported here indicate a significant role of α2 NKA in regulating central LPS effects, with implications in the associated neuroinflammatory processes.
Directional Exosome Proteomes Reflect Polarity-Specific Functions in Retinal Pigmented Epithelium Monolayers
Authors: Klingeborn Mikael , Dismuke W. Michael , Skiba Nikolai P. , Kelly Una , Stamer W. Daniel , Bowes Rickman Catherine
Published: July 2017

The retinal pigmented epithelium (RPE) forms the outer blood-retinal barrier in the eye and its polarity is responsible for directional secretion and uptake of proteins, lipoprotein particles and extracellular vesicles (EVs). Such a secretional division dictates directed interactions between the systemic circulation (basolateral) and the retina (apical). Our goal is to define the polarized proteomes and physical characteristics of EVs released from the RPE. Primary cultures of porcine RPE cells were differentiated into polarized RPE monolayers on permeable supports. EVs were isolated from media bathing either apical or basolateral RPE surfaces, and two subpopulations of small EVs including exosomes, and dense EVs, were purified and processed for proteomic profiling. In parallel, EV size distribution and concentration were determined. Using protein correlation profiling mass spectrometry, a total of 631 proteins were identified in exosome preparations, 299 of which were uniquely released apically, and 94 uniquely released basolaterally. Selected proteins were validated by Western blot. The proteomes of these exosome and dense EVs preparations suggest that epithelial polarity impacts directional release. These data serve as a foundation for comparative studies aimed at elucidating the role of exosomes in the molecular pathophysiology of retinal diseases and help identify potential therapeutic targets and biomarkers.
Fate and Transport of Shale-derived, Biogenic Methane
Authors: Hendry M. Jim , Schmeling Erin E. , Barbour S. Lee , Huang M. , Mundle Scott O. C.
Published: July 2017

Natural gas extraction from unconventional shale gas reservoirs is the subject of considerable public debate, with a key concern being the impact of leaking fugitive natural gases on shallow potable groundwater resources. Baseline data regarding the distribution, fate, and transport of these gases and their isotopes through natural formations prior to development are lacking. Here, we define the migration and fate of CH4 and δ13C-CH4 from an early-generation bacterial gas play in the Cretaceous of the Williston Basin, Canada to the water table. Our results show the CH4 is generated at depth and diffuses as a conservative species through the overlying shale. We also show that the diffusive fractionation of δ13C-CH4 (following glaciation) can complicate fugitive gas interpretations. The sensitivity of the δ13C-CH4 profile to glacial timing suggests it may be a valuable tracer for characterizing the timing of geologic changes that control transport of CH4 (and other solutes) and distinguishing between CH4 that rapidly migrates upward through a well annulus or other conduit and CH4 that diffuses upwards naturally. Results of this study were used to provide recommendations for designing baseline investigations.
Full left ventricular coverage is essential for the accurate quantification of the area-at-risk by T1 and T2 mapping
Authors: Bulluck Heerajnarain , Bryant Jennifer A. , Lim Mei Xing , Tan Xiao Wei , Ramlall Manish , Francis Rohin , Kotecha Tushar , Cabrera-Fuentes Hector A. , Knight Daniel S. , Fontana Marianna , Moon James C. , Hausenloy Derek J.
Published: July 2017

T2-weighted cardiovascular magnetic resonance (CMR) using a 3-slice approach has been shown to accurately quantify the edema-based area-at-risk (AAR) in ST-segment elevation myocardial infarction (STEMI). We aimed to compare the performance of a 3-slice approach to full left ventricular (LV) coverage for the AAR by T1 and T2 mapping and MI size. Forty-eight STEMI patients were prospectively recruited and underwent a CMR at 4 ± 2 days. There was no difference between the AARfull LV and AAR3-slices by T1 (P = 0.054) and T2-mapping (P = 0.092), with good correlations but small biases and wide limits of agreements (T1-mapping: N = 30, R2 = 0.85, bias = 1.7 ± 9.4% LV; T2-mapping: N = 48, R2 = 0.75, bias = 1.7 ± 12.9% LV). There was also no significant difference between MI size3-slices and MI sizefull LV (P = 0.93) with an excellent correlation between the two (R2 0.92) but a small bias of 0.5% and a wide limit of agreement of ±7.7%. Although MSI was similar between the 2 approaches, MSI3-slices performed poorly when MSI was <0.50. Furthermore, using AAR3-slices and MI sizefull LV resulted in ‘negative’ MSI in 7/48 patients. Full LV coverage T1 and T2 mapping are more accurate than a 3-slice approach for delineating the AAR, especially in those with MSI < 0.50 and we would advocate full LV coverage in future studies.