bluuebunny/crossref_metadata_2025 huggingface dataset:
Diversity of Endophytic Microbes in Taxus yunnanensis and Their Potential for Plant Growth Promotion and Taxane Accumulation
Authors: Liu Qiao , Li Ludan , Chen Yujie , Wang Sai , Xue Lina , Meng Weiying , Jiang Jihong , Cao Xiaoying
Published: June 2023

Taxus spp. are ancient tree species that have survived from the Quaternary glacier period, and their metabolites, such as taxol, have been used as anticancer drugs globally. Plant–endophytic microbial interaction plays a crucial role in exerting a profound impact on host growth and secondary metabolite synthesis. In this study, high-throughput sequencing was employed to explore endophytic microbial diversity in the roots, stems, and leaves of the Taxus yunnanensis (T. yunnanensis). The analysis revealed some dominant genera of endophytic bacteria, such as Pseudomonas, Neorhizobium, Acidovorax, and Flavobacterium, with Cladosporium, Phyllosticta, Fusarium, and Codinaeopsis as prominent endophytic fungi genera. We isolated 108 endophytic bacteria and 27 endophytic fungi from roots, stems, and leaves. In vitro assays were utilized to screen for endophytic bacteria with growth-promoting capabilities, including IAA production, cellulase, siderophore production, protease and ACC deaminase activity, inorganic phosphate solubilization, and nitrogen fixation. Three promising strains, Kocuria sp. TRI2-1, Micromonospora sp. TSI4-1, and Sphingomonas sp. MG-2, were selected based on their superior growth-promotion characteristics. These strains exhibited preferable plant growth promotion when applied to Arabidopsis thaliana growth. Fermentation broths of these three strains were also found to significantly promote the accumulation of taxanes in T. yunnanensis stem cells, among which strain TSI4-1 demonstrated outstanding increase potentials, with an effective induction of taxol, baccatin III, and 10-DAB contents. After six days of treatment, the contents of these metabolites were 3.28 times, 2.23 times, and 2.17 times the initial amounts, reaching 8720, 331, and 371 ng/g of dry weight of stem cells, respectively. These findings present new insight into the industrialization of taxol production through Taxus stem cell fermentation, thereby promoting the conservation of wild Taxus resources by maximizing their potential economic benefits.
The Mobilome-Enriched Genome of the Competence-Deficient Streptococcus pneumoniae BM6001, the Original Host of Integrative Conjugative Element Tn5253, Is Phylogenetically Distinct from Historical Pneumococcal Genomes
Authors: Colombini Lorenzo , Cuppone Anna Maria , Tirziu Mariana , Lazzeri Elisa , Pozzi Gianni , Santoro Francesco , Iannelli Francesco
Published: June 2023

Streptococcus pneumoniae is an important human pathogen causing both mild and severe diseases. In this work, we determined the complete genome sequence of the S. pneumoniae clinical isolate BM6001, which is the original host of the ICE Tn5253. The BM6001 genome is organized in one circular chromosome of 2,293,748 base pairs (bp) in length, with an average GC content of 39.54%; the genome harbors a type 19F capsule locus, two tandem copies of pspC, the comC1-comD1 alleles and the type I restriction modification system SpnIII. The BM6001 mobilome accounts for 15.54% (356,521 bp) of the whole genome and includes (i) the ICE Tn5253 composite; (ii) the novel IME Tn7089; (iii) the novel transposon Tn7090; (iv) 3 prophages and 2 satellite prophages; (v) 5 genomic islands (GIs); (vi) 72 insertion sequences (ISs); (vii) 69 RUPs; (viii) 153 BOX elements; and (ix) 31 SPRITEs. All MGEs, except for the GIs, produce excised circular forms and attB site restoration. Tn7089 is 9089 bp long and contains 11 ORFs, of which 6 were annotated and code for three functions: integration/excision, mobilization and adaptation. Tn7090 is 9053 bp in size, flanked by two copies of ISSpn7, and contains seven ORFs organized as a single transcriptional unit, with genes encoding for proteins likely involved in the uptake and binding of Mg2+ cations in the adhesion to host cells and intracellular survival. BM6001 GIs, except for GI-BM6001.4, are variants of the pneumococcal TIGR4 RD5 region of diversity, pathogenicity island PPI1, R6 Cluster 4 and PTS island. Overall, prophages and satellite prophages contain genes predicted to encode proteins involved in DNA replication and lysogeny, in addition to genes encoding phage structural proteins and lytic enzymes carried only by prophages. ΦBM6001.3 has a mosaic structure that shares sequences with prophages IPP69 and MM1 and disrupts the competent comGC/cglC gene after chromosomal integration. Treatment with mitomycin C results in a 10-fold increase in the frequency of ΦBM6001.3 excised forms and comGC/cglC coding sequence restoration but does not restore competence for genetic transformation. In addition, phylogenetic analysis showed that BM6001 clusters in a small lineage with five other historical strains, but it is distantly related to the lineage due to its unique mobilome, suggesting that BM6001 has progressively accumulated many MGEs while losing competence for genetic transformation.
Assessing the Feasibility of Employing a Combination of a Bacteriophage-Derived Endolysin and Spore Germinants to Treat Relapsing Clostridioides difficile Infection
Authors: Alyahya Khalid , Baillie Les
Published: June 2023

Clostridioides difficile is a Gram-positive, anaerobic, spore-forming bacillus and is a major cause of healthcare-associated infections. Whereas the vegetative form of the pathogen is susceptible to treatment with antibiotics, its ability to persist in the gut as antibiotic-resistant spores means that reinfection can occur in cases were the individual fails to re-establish a protective microflora. Bacteriophages and their lysins are currently being explored as treatment options due to their specificity, which minimizes the disruption to the other members of the gut microflora that are protective. The feasibility of employing recombinant endolysins to target the vegetative form of C. difficile has been demonstrated in animal models. In this study, we cloned and expressed the enzyme active domain of LysCD6356 and confirmed its ability to lyse the vegetative forms of a diverse range of clinical isolates of C. difficile, which included members of the hypervirulent 027 ribotype. Lytic activity was adversely affected by calcium, which is naturally found in the gut and is released from the spore upon germination. Our results suggests that a strategy in which the triggering of spore germination is separated in time from the application of the lysin could be developed as a strategy to reduce the risk of relapsing C. difficile infections.
Recent Advances in the Use of Probiotics to Improve Meat Quality of Small Ruminants: A Review
Authors: Saha Sudeb , Fukuyama Kohtaro , Debnath Marina , Namai Fu , Nishiyama Keita , Kitazawa Haruki
Published: June 2023

Meat from small ruminants is considered a high quality and delicacy product in many countries. Several benefits have been perceived from probiotics as dietary supplements, such as improved carcass weight, color, tenderness, flavor, muscle fiber structure, water-holding capacity, and healthy fatty acid profile of the meat. Thus, the present review focuses on the effect of probiotics on improving the quality of meat from small ruminants. Though many benefits have been associated with the use of probiotics, the findings of all the considered articles are not always consistent, and the mechanisms behind improving meat quality are not appropriately defined. This variability of findings could be due to the use of different probiotic strains, dosage rates, number of days of experiment, nutrition, breed, age, and health status of the animals. Therefore, future research should emphasize specific strains, optimal dose and days of administration, route, and mechanisms for the specific probiotic strains to host. This review provides a comprehensive overview of the use of probiotics for small ruminants and their impact on meat quality.
Rhizobium Soaking Promoted Maize Growth by Altering Rhizosphere Microbiomes and Associated Functional Genes
Authors: Li Zhao , Chi Yu , Su Xianyan , Ye Zhenghe , Ren Xuexiang
Published: June 2023

Rhizobium is a Gram-negative bacterium, which dissolves minerals, produces growth hormones, promotes root growth, and protects plants from different soil-borne pathogens. In the present study, roots, stalks, and fresh weight of maize (Zea mays L.) were significantly increased after soaking in Bradyrhizobium japonicum compared with the control. Subsequently, transcriptome sequencing results of the whole maize plant soaked in B. japonicum showed that multiple growth and development-related genes were up-regulated more than 100-fold compared to the control. Furthermore, the abundance of plant growth promoting bacteria, such as Acidobacteria Subgroup_6 and Chloroflexi KD4-96, were increased significantly. On the contrary, the abundance of multiple pathogens, such as Curvularia, Fusarium and Mycocentrospora, were significantly decreased. Moreover, inoculation with B. japonicum could inhibit the infection of the pathogen Fusarium graminearum in maize. These results suggest that soaking seeds in B. japonicum may affect the expression of maize growth and development-related genes as the bacteria changes the soil microorganism community structure. These findings may help to expand the application of B. japonicum in crop production and provide new opportunities for food security.
Microbial Communities Affected by Hydraulic Fracturing and Environmental Factors within an In Situ Coal Reservoir
Authors: Li Yang , Chen Jian , Tang Shuheng , Xi Zhaodong
Published: June 2023

The rise of coalbed methane bioengineering enables the conversion and utilization of carbon dioxide through microbial action and the carbon cycle. The environment of underground coal reservoirs is the result of a comprehensive effort by microorganisms. Some studies on reservoir microorganisms have progressed in laboratory conditions. However, it does not replicate the interaction between microorganisms and the environment on site. Hydraulic fracturing is an engineering technology to improve the natural permeability of tight reservoirs and is also a prerequisite for increasing biomethane production. In addition to expanding the pore and fracture systems of coal reservoirs, hydraulic fracturing also improves the living conditions of microbial communities in underground space. The characteristics of microbial communities in the reservoir after hydraulic fracturing are unclear. To this end, we applied the 16S rRNA sequencing technique to coalbed methane production water after hydraulic fracturing south of the Qinshui Basin to analyze the microbial response of the hydraulic fracturing process in the coal reservoir. The diversity of microbial communities associated with organic degradation was improved after hydraulic fracturing in the coal reservoir. The proportion of Actinobacteria in the reservoir water of the study area increased significantly, and the abundance of Aminicenantes and Planctomycetes increased, which do not exist in non-fracturing coalbed methane wells or exist at very low abundance. There are different types of methanogens in the study area, especially in fracturing wells. Ecological factors also determine the metabolic pathway of methanogens in coal seams. After hydraulic fracturing, the impact on the reservoir’s microbial communities remains within months. Hydraulic fracturing can strengthen the carbon circulation process, thereby enhancing the block’s methane and carbon dioxide circulation. The study provides a unique theoretical basis for microbially enhanced coalbed methane.
Nano-Cavities within Nano-Zeolites: The Influencing Factors of the Fabricating Process on Their Catalytic Activities
Authors: Fu Kairui , Li Geng , Xu Fulin , Dai Tiantong , Su Wen , Wang Hao , Li Tianduo , Wang Yunan , Wang Jingui
Published: June 2023

Titanium silicalite-1 (TS-1) is a milestone heterogeneous catalyst with single-atom tetrahedral titanium incorporated into silica framework for green oxidation reactions. Although TS-1 catalysts have been industrialized, the strategy of direct hydrothermal synthesis usually produces catalysts with low catalytic activities, which has still puzzled academic and industrial scientists. Post-treatment processes were widely chosen and were proven to be an essential process for the stable production of the high-activity zeolites with hollow structures. However, the reasons why post-treatment processes could improve catalytic activity are still not clear enough. Here, high-performance hollow TS-1 zeolites with nano-sized crystals and nano-sized cavities were synthesized via post-treatment of direct-synthesis nano-sized TS-1 zeolites. The influencing factors of the fabricating processes on their catalytic activities were investigated in detail, including the content of alkali metal ions, the state of titanium centers, hydrophilic/hydrophobic properties, and accessibility of micropores. The post-treatment processes could effectively solve these adverse effects to improve catalytic activity and to stabilize production. These findings contribute to the stable preparation of high-performance TS-1 catalysts in both factories and laboratories.
Synthesis, Characterization and Performance of Materials for a Sustainable Future
Authors: Vakros John , Hapeshi Evroula , Cannilla Catia , Bonura Giuseppe
Published: June 2023

Today, sustainability represents the key factor for economic progress in compliance with social advancement and environmental protection, driving innovation in materials, processes and technologies [...]
Combination of CNTs with Classical Drugs for Treatment in Human Colorectal Adenocarcinoma (HT-29) Cell Line
Authors: Abreu Sara , Vale Nuno , Soares Olívia Salomé G. P.
Published: June 2023

Due to the increase in new types of cancer cells and resistance to drugs, conventional cancer treatments are sometimes insufficient. Therefore, an alternative is to apply nanotechnology to biomedical areas, minimizing side effects and drug resistance and improving treatment efficacy. This work aims to find a promising cancer treatment in the human colorectal adenocarcinoma cell line (HT-29) to minimize the viability of cells (IC50) by using carbon nanotubes (CNTs) combined with different drugs (5-fluorouracil (5-FU) and two repurposing drugs—tacrine (TAC) and ethionamide (ETA). Several CNT samples with different functional groups (-O, -N, -S) and textural properties were prepared and characterized by elemental and thermogravimetry analysis, size distribution, and textural and temperature programmed desorption. The samples that interacted most with the drugs and contributed to improving HT-29 cell treatment were samples doped with nitrogen and sulfur groups (CNT-BM-N and CNT-H2SO4-BM) with IC50 1.98 and 2.50 µmol∙dm−3 from 5-FU and 15.32 and 15.81 µmol∙dm−3 from TAC. On the other hand, ETA had no activity, even combined with the CNTs. These results allow us to conclude that the activity was improved for both 5-FU and TAC when combined with CNTs.
Malnutrition, Depression, Poor Sleep Quality, and Difficulty Falling Asleep at Night Are Associated with a Higher Risk of Cognitive Frailty in Older Adults during the COVID-19 Restrictions
Authors: Griffiths Jiranan , Seesen Mathuramat , Sirikul Wachiranun , Siviroj Penprapa
Published: June 2023

The COVID-19 restrictions, such as social isolation and disruption of daily routines, can have detrimental effects, including increased stress, anxiety, sleep disturbance, and physical and cognitive decline among older adults. This study aimed to examine the association between nutritional status, depression, sleep quality, falling asleep at night, and cognitive frailty (CF) among older Thai adults during the COVID-19 pandemic. This cross-sectional study included 408 older adults with an average age of 70.54 (5.49) years. CF was determined using Fried’s frailty phenotype and the Montreal Cognitive Assessment Basic. The Mini Nutritional Assessment-Short Form, Pittsburgh Sleep Quality Index, and geriatric depression assessment were used for assessment. Multiple logistic regression analysis demonstrated that participants who were malnourished (OR 3.786; 95%CI 1.719–8.335), depressed (OR 5.003; 95%CI 2.399–10.434), had poor sleep quality (OR 1.613; 95%CI 1.041–2.500), and engaged in difficulty falling asleep (OR 1.809; 95%CI 1.022–3.203) had a higher risk of CF compared to those who did not exhibit these factors. Therefore, malnutrition, depression, poor sleep quality, and difficulty falling asleep were identified as risk factors for CF among older adults in Thailand linked to the impact of the COVID-19 pandemic. It is crucial to develop interventions to prevent CF resulting from the mentioned variables.
Plant-Based Dietary Patterns and Cardiovascular Disease Risk in Australians: Protocol for a Cross-Sectional Study
Authors: Ferguson Jessica , Austin Grace , Oldmeadow Christopher , Garg Manohar
Published: June 2023

Plant-based diets (PBDs) emphasise higher intakes of plant foods and lower intakes of animal foods, and they have been associated with reduced cardiovascular morbidity/mortality and lower cardiovascular disease (CVD) risk factors. Evidence is limited regarding the dietary profile, diet quality, and nutritional adequacy of PBDs, including their impact on CVD risk compared with traditional meat-eating diets in Australians. The PBD Study (PBDS) is a cross-sectional study that will recruit 240 adults from the Hunter region (NSW) without known CVD who are habitually consuming vegan (no animal flesh/animal products), lacto-ovo vegetarian (dairy and/or eggs only), pesco-vegetarian (fish/seafood only), or semi-vegetarian (minimal animal flesh) diets or are a regular meat-eater. To investigate dietary profile, diet quality, nutritional adequacy, and CVD risk, questionnaires (medical history, demographics, and physical activity), blood samples (biomarkers), physical measures (anthropometry, blood pressure, body composition, and bone density), and dietary intake (food frequency questionnaire and diet history) will be collected. One-way ANOVA and Kruskal–Wallis tests will compare the CVD risk and other quantitative measures, and Chi-square or Fisher’s Exact tests will be used for qualitative data. Directed acyclic graphs will determine the confounding variables, and linear regression and mediation analyses will account for the confounders and estimate the effect of dietary patterns on CVD risk. p-values will be adjusted using the Benjamini–Hochberg method to control the False Discovery Rate to 5%.
A 20-Country Comparative Assessment of the Effectiveness of Nutri-Score vs. NutrInform Battery Front-of-Pack Nutritional Labels on Consumer Subjective Understanding and Liking
Authors: He Jun , Mazzù Marco Francesco , Baccelloni Angelo
Published: June 2023

The incidence of overweight and obesity has generated significant concerns among European consumers and institutions. As part of a set of measures undertaken, the European Union (EU) called for one harmonized mandatory front-of-pack nutritional label (FOPL) to improve consumer food nutritional knowledge and encourage healthier and more informed food choices. Different types of FOPLs, ranging from nutrient-specific labels—such as the NutrInform Battery—to summary labels—such as the Nutri-Score—have been developed and introduced in different markets, reporting different degrees of effectiveness in terms of understanding. The aim of this study is to provide actionable insights by analyzing a specific part of the complex consumers’ decision-making process in food when aided by FOPLs. Adopting a between-subject experiment on a sample of 4560 respondents in 20 EU member countries, the study compares the consumer subjective understanding and liking of two labels currently under examination by the EU bodies, the NutrInform Battery and the Nutri-Score. At an aggregated level, the results show that NutrInform Battery is more effective than Nutri-Score in improving consumer subjective understanding and leads to a higher liking towards the label. A detailed by-country analysis highlights either a superiority or a parity of NutrInform Battery for subjective understanding and liking. Theoretically, this study, through a large panel of respondents, adds the fundamental perspective on subjective understanding, complementing the findings of extant research on objective understanding, and further clarifies the role of liking as a complementary element in the food decision-making process toward heathier and more informed food choices. This might be of significant relevance in providing additional evidence that can be used by policymakers in their attempt toward the selection of a uniform FOPL at EU level.
Exploring the Impact of Sociodemographic Characteristics and Health Literacy on Adherence to Dietary Recommendations and Food Literacy
Authors: Forray Alina Ioana , Coman Mădălina Adina , Cherecheș Răzvan Mircea , Borzan Cristina Maria
Published: June 2023

This study investigates food literacy-related abilities and adherence to dietary recommendations in relation to sociodemographic characteristics and health-related features (health literacy, self-rated health and morbidity) in the North-Western region of Romania. This is a secondary analysis of cross-sectional data collected in 2019 from a representative and randomised sample of 1572 individuals. A questionnaire was employed to record participants’ sociodemographic characteristics, food-related and health-related features. Most participants were non-adherent to dietary recommendations for fruit and vegetables (83.5%), fish and seafood (61.3%), and water intake (67.9%). However, most participants reported an adequate ability to understand the connection between nutrition and health (89.1%), to distinguish between healthy and less healthy options (84.4%), and to acquire nutrition information (75.6%). Non-adherence to dietary recommendations and low food literacy abilities were more prevalent in disadvantaged groups (older age, rural settings, retirement or social welfare, low educational attainment, formerly married). Health literacy was negatively associated with not adhering to dietary recommendations and poor self-rated food literacy abilities. The study suggests that low socioeconomic status negatively impacts food literacy and adherence to dietary recommendations among Romanian adults. Identifying target populations to improve food-related abilities and health literacy can aid public health services in improving health outcomes.
Preventive Treatment with Astaxanthin Microencapsulated with Spirulina Powder, Administered in a Dose Range Equivalent to Human Consumption, Prevents LPS-Induced Cognitive Impairment in Rats
Authors: Martin Miquel , Pusceddu Matteo M. , Teichenné Joan , Negra Teresa , Connolly Alan , Escoté Xavier , Torrell Galceran Helena , Cereto Massagué Adrià , Samarra Mestre Iris , del Pino Rius Antoni , Romero-Gimenez Jordi , Egea Cristina , Alcaide-Hidalgo Juan Maria , del Bas Josep Maria
Published: June 2023

Cognitive alterations are a common feature associated with many neurodegenerative diseases and are considered a major health concern worldwide. Cognitive alterations are triggered by microglia activation and oxidative/inflammatory processes in specific areas of the central nervous system. Consumption of bioactive compounds with antioxidative and anti-inflammatory effects, such as astaxanthin and spirulina, can help in preventing the development of these pathologies. In this study, we have investigated the potential beneficial neuroprotective effects of a low dose of astaxanthin (ASX) microencapsulated within spirulina (ASXSP) in female rats to prevent the cognitive deficits associated with the administration of LPS. Alterations in memory processing were evaluated in the Y-Maze and Morris Water Maze (MWM) paradigms. Changes in microglia activation and in gut microbiota content were also investigated. Our results demonstrate that LPS modified long-term memory in the MWM and increased microglia activation in the hippocampus and prefrontal cortex. Preventive treatment with ASXSP ameliorated LPS-cognitive alterations and microglia activation in both brain regions. Moreover, ASXSP was able to partially revert LPS-induced gut dysbiosis. Our results demonstrate the neuroprotective benefits of ASX when microencapsulated with spirulina acting through different mechanisms, including antioxidant, anti-inflammatory and, probably, prebiotic actions.
The Effects of a Western Diet vs. a High-Fiber Unprocessed Diet on Health Outcomes in Mice Offspring
Authors: Herzl Elizabeth , Schmitt Emily E. , Shearrer Grace , Keith Jill F.
Published: June 2023

Diet influences critical periods of growth, including gestation and early development. We hypothesized that a maternal/early life diet reflecting unprocessed dietary components would positively affect offspring metabolic and anthropometric parameters. Using 9 C57BL-6 dams, we simulated exposure to a Western diet, a high-fiber unprocessed diet (HFUD), or a control diet. The dams consumed their respective diets (Western [n = 3], HFUD [n = 3], and control [n = 3]) through 3 weeks of pregnancy and 3 weeks of weaning; their offspring consumed the diet of their mother for 4.5 weeks post weaning. Measurements included dual X-ray absorptiometry (DEXA) scans, feed consumption, body weight, blood glucose, and insulin and glycated hemoglobin (HbA1c) in the offspring. Statistical analyses included one-way ANOVA with Tukey’s post hoc analysis. The offspring DEXA measures at 5 and 7.5 weeks post parturition revealed higher lean body mass development in the HFUD and control diet offspring compared to the Western diet offspring. An analysis indicated that blood glucose (p = 0.001) and HbA1c concentrations (p = 0.002) were lower among the HFUD offspring compared to the Western and control offspring. The results demonstrate that diet during gestation and early life consistent with traditional diet patterns may influence hyperglycemia and adiposity in offspring.
Vindoline Exhibits Anti-Diabetic Potential in Insulin-Resistant 3T3-L1 Adipocytes and L6 Skeletal Myoblasts
Authors: Shijina Beegum Noorjahan , Radhika Achuthan , Sherin Sainulabdeen , Biju Prabath Gopalakrishnan
Published: June 2023

Type 2 diabetes mellitus (T2DM) emerged as a major health care concern in modern society, primarily due to lifestyle changes and dietary habits. Obesity-induced insulin resistance is considered as the major pathogenic factor in T2DM. In this study, we investigated the effect of vindoline, an indole alkaloid of Catharanthus roseus on insulin resistance (IR), oxidative stress and inflammatory responses in dexamethasone (IR inducer)-induced dysfunctional 3T3-L1 adipocytes and high-glucose-induced insulin-resistant L6-myoblast cells. Results showed that dexamethasone-induced dysfunctional 3T3-L1 adipocytes treated with different concentrations of vindoline significantly enhanced basal glucose consumption, accompanied by increased expression of GLUT-4, IRS-1 and adiponectin. Similarly, vindoline-treated insulin-resistant L6 myoblasts exhibited significantly enhanced glycogen content accompanied with upregulation of IRS-1 and GLUT-4. Thus, in vitro studies of vindoline in insulin resistant skeleton muscle and dysfunctional adipocytes confirmed that vindoline treatment significantly mitigated insulin resistance in myotubes and improved functional status of adipocytes. These results demonstrated that vindoline has the potential to be used as a therapeutic agent to ameliorate obesity-induced T2DM-associated insulin resistance profile in adipocytes and skeletal muscles.
Association between Dietary Inflammatory Index and Gastric Adenocarcinoma: A Multicenter Case-Control Study in Brazil
Authors: Silva Alex Richard Costa , Guandalini Valdete Regina , Pereira Taísa Sabrina Silva , Zhao Longgang , Wirth Michael D. , Hébert James R. , Fernandes Gisele Aparecida , de Assumpção Paulo Pimentel , Barbosa Mônica Santiago , Curado Maria Paula
Published: June 2023

Background: Few studies have evaluated the association between diet-related inflammation and gastric adenocarcinoma (GA) and evidence is scarce in Brazil. This study evaluated the association between a pro-inflammatory diet and GA. Methods: A multicenter case–control study was conducted in Brazil. A total of 1645 participants—492 cases, 377 endoscopy controls, and 776 hospital controls—were included. Energy-adjusted Dietary Inflammatory Index (E-DIITM) scores were derived from a validated food frequency questionnaire. We used binary and multinomial logistic regression models for the analysis of total GA, and its subtypes (cardia and non-cardia, intestinal, and diffuse histological subtypes). Results: In cases versus endoscopy controls, a pro-inflammatory diet, estimated by higher E-DII scores, was associated with a higher risk GA (ORQ4vsQ1: 2.60, 1.16–5.70), of non-cardia GA (OR: 2.90, 1.06–7.82), and diffuse subtype (OR: 3.93, 1.59–9.70). In cases versus hospital controls, higher E-DII scores were associated with a higher risk of GA (OR: 2.70, 1.60–4.54), of cardia GA (OR: 3.31, 1.32–8.24), non-cardia GA (OR: 2.97, 1.64–5.39), and both intestinal (OR: 2.82, 1.38–5.74) and diffuse GA (OR: 2.50, 1.54–5.11) subtypes. Conclusions: This study provides evidence that a pro-inflammatory diet is associated with an increased risk of GA in Brazil. E-DII requires the inclusion of sodium due to its importance in carcinogenesis.
Effects of Consuming Ounce-Equivalent Portions of Animal- vs. Plant-Based Protein Foods, as Defined by the Dietary Guidelines for Americans on Essential Amino Acids Bioavailability in Young and Older Adults: Two Cross-Over Randomized Controlled Trials
Authors: Connolly Gavin , Hudson Joshua L. , Bergia Robert E. , Davis Eric M. , Hartman Austin S. , Zhu Wenbin , Carroll Chad C. , Campbell Wayne W.
Published: June 2023

Background: The Dietary Guidelines for Americans (DGA) recommends consuming a variety of “Protein Foods” based on “ounce-equivalent” (oz-eq) portions. No study has assessed the same oz-eq portions of animal- vs. plant-based protein foods on essential amino acid (EAA) bioavailability for protein anabolism in young and older adults. Objectives: We assessed the effects of consuming two oz-eq portions of pork, eggs, black beans, and almonds on postprandial EAA bioavailability in young and older adults. Methods: We conducted two investigator-blinded, randomized crossover trials in young (n = 30; mean age ± SD: 26.0 ± 4.9 y) and older adults (n = 25; mean age ± SD: 64.2 ± 6.6 y). Participants completed four testing sessions where they consumed a standardized meal with two oz-eq of either unprocessed lean pork, whole eggs, black beans, or sliced almonds. Blood samples were taken at baseline and 30, 60, 120, 180, 240, and 300 min postprandially. Plasma EAA bioavailability was based on postprandial integrated positive areas under the curve. Results: Participant age did not affect EAA bioavailability among the four protein foods tested. Two oz-eq portions of pork (7.36 g EAA) and eggs (5.38 g EAA) resulted in greater EAA bioavailability than black beans (3.02 g EAA) and almonds (1.85 g EAA) in young and older adults, separately or combined (p < 0.0001 for all). Pork resulted in greater EAA bioavailability than eggs in young adults (p < 0.0001), older adults (p = 0.0007), and combined (p < 0.0001). There were no differences in EAA bioavailability between black beans and almonds. Conclusions: The same “oz-eq” portions of animal- and plant-based protein foods do not provide equivalent EAA content and postprandial bioavailability for protein anabolism in young and older adults.
Photodynamic Therapy of Oral Cancer and Novel Liposomal Photosensitizers
Authors: Düzgüneş Nejat , Piskorz Jaroslaw , Skupin-Mrugalska Paulina , Yıldırım Metin , Sessevmez Melike , Cheung Jennifer
Published: June 2023

Photodynamic therapy facilitates the selective destruction of cancer tissue by utilizing a photosensitizer drug, the light near the absorbance wavelength of the drug, and oxygen. Methylene Blue, 5-aminolevulinic acid (the precursor of the photosensitizer, protoporphyrin IX), porphyrin, Foscan, Chlorin e6, and HPPH have been used successfully as photosensitizers in the treatment of oral verrucous hyperplasia, oral leukoplakia, oral lichen planus, and head and neck squamous cell carcinoma. “Theranostic” liposomes can deliver a contrast agent for magnetic resonance imaging and a photosensitizer for the image-guided photodynamic therapy of head and neck cancer. Liposomes incorporating photosensitizers can be targeted to cell surface markers overexpressed on cancer cells. Novel porphyrinoids have been developed in our laboratories that are highly effective as photosensitizers. Tribenzoporphyrazines encapsulated in cationic liposomes have produced IC50 values up to 50 times lower compared to the free photosensitizers. It is anticipated that targeting these drugs to cancer stem cells, using upconversion nanoparticles for the near-infrared irradiation of tumors to activate the photosensitizers, and overcoming tumor hypoxia will enhance the efficacy of photodynamic therapy of tumors accessible to light sources.
Resident Physicians’ Knowledge and Preparedness Regarding Human Monkeypox: A Cross-Sectional Study from Saudi Arabia
Authors: Shafei Ali Mohammad , Al-Mosaa Khalid M. , Alshahrani Najm Z. , ALAmmari Mohammed Hassan Mohammed , Almuhlafi Mashael Obaid Othman , Draim Nasser Hassan Awdah Al , Alwadie Afnan Misfer , Alghrab Abdullah Ibrahim
Published: June 2023

This study aimed to evaluate knowledge about monkeypox and confidence in dealing with monkeypox diagnosis and management among resident physicians in the Asir region of Saudi Arabia. The data of this cross-sectional study were collected online through a structured questionnaire (N = 315). Knowledge about monkeypox was assessed by 24 questions and a three-item scale assessed confidence in managing monkeypox cases. Multiple logistic regression analysis was performed to assess the associations. Overall, two-thirds of the respondents (67.3%) showed good knowledge about monkeypox and the rest of them (32.7%) had poor knowledge. Respondents who received a copy of the Saudi MOH protocol for monkeypox and attended any conference or lecture about monkeypox were more likely to have good knowledge about monkeypox. Moreover, almost two-thirds of the participants were not confident that they could diagnose or manage the monkeypox. Respondents who received information about monkeypox during residency or medical school had higher confidence in managing monkeypox cases. It can be concluded that the Saudi healthcare system has scope to take necessary steps to contain the monkeypox endemic. The Saudi MOH should arrange conferences and educational programs on monkeypox so that healthcare professionals can improve their knowledge and be confident in the management and diagnosis of monkeypox cases.
Comparison of Three Serologic Tests for the Detection of Anti-Coxiella burnetii Antibodies in Patients with Q Fever
Authors: França Danilo Alves de , Mioni Mateus de Souza Ribeiro , Fornazari Felipe , Rodrigues Nássarah Jabur Lot , Polido Lucas Roberto Ferreira , Appolinario Camila Michele , Ribeiro Bruna Letícia Devidé , Duré Ana Íris de Lima , Silva Marcos Vinicius Ferreira , Richini-Pereira Virgínia Bodelão , Langoni Helio , Megid Jane
Published: June 2023

The performance of a commercial immunofluorescence assay (IFA commercial), an in-house immunofluorescence assay (IFA in-house) and an indirect enzyme-linked immunosorbent assay (ELISA) were evaluated in the detection of antibodies anti-C. burnetii in the serum of Q fever patients and persons without the disease. For the study, seropositive and seronegative samples for Q fever (n = 200) from a serum bank of the Instituto Adolfo Lutz in Brazil were used. Commercial IFA was considered in this study as the gold standard for diagnosing Q fever. The in-house IFA demonstrated good agreement with the commercial test, showing high sensitivity (91%) and specificity (97%) compared to the gold standard, with a Kappa coefficient of 0.8954. The indirect ELISA test showed lower agreement with the gold standard, showing low sensitivity (67%), although the specificity of the technique was high (97%) and the Kappa coefficient was moderate (0.6631). In-house IFA is an excellent alternative for diagnosing Q fever.
The Potential Protective Role of Naringenin against Dasatinib-Induced Hepatotoxicity
Authors: Alanazi Ahmed Z. , Alhazzani Khalid , Alrewily Salah Q. , Aljerian Khaldoon , Algahtani Mohammad M. , Alqahtani Qamraa H. , Haspula Dhanush , Alhamed Abdullah S. , Alqinyah Mohammed , Raish Mohammad
Published: June 2023

Dasatinib (DASA) is a novel tyrosine kinase inhibitor, approved for leukemia treatment. However, the long-term use of DASA induces several complications, especially liver damage. On the other hand, Naringenin (NGN) is a potent antioxidant and anti-inflammatory agent which is known to exert protective effects in several liver disease animal models. Yet, the effect of NGN on DASA-induced hepatotoxicity has not been examined. This study investigated the hepatoprotective effects of NGN against DASA-induced acute liver injury, using a mouse model. The mice were given NGN (50, 100, and 200 mg/kg po) or saline for 7 days, followed by DASA on the eighth day (25 mg/kg p.o.). DASA treatment alone was found to cause overexpression of proinflammatory cytokines, such as interleukin-10 (IL-10), tumor necrosis factor-alpha (TNF-α), and malonyl aldehyde (MDA), whereas attenuation of antioxidant genes including superoxide dismutase (SOD), catalase (CAT), glutathione S-transferase (GST), and glutathione peroxidase (GPx). Interestingly, a pretreatment with NGN + DASA resulted in minimizing the proinflammatory mediators and restoring the levels of antioxidant genes. In addition, there was evidence of necro-inflammatory changes in histopathological findings in the liver samples after DASA administration which remarkably reduced with NGN + DASA. Thus, this study revealed that NGN could minimize the hepatotoxicity induced by DASA by providing anti-inflammatory and antioxidant protection.
Triple Negative Breast Cancer Treatment Options and Limitations: Future Outlook
Authors: Obidiro Onyinyechi , Battogtokh Gantumur , Akala Emmanuel O.
Published: June 2023

Triple negative breast cancer (TNBC) has a negative expression of estrogen receptors (ER), progesterone receptors (PR), and human epidermal growth factor receptors (HER2). The survival rate for TNBC is generally worse than other breast cancer subtypes. TNBC treatment has made significant advances, but certain limitations remain. Treatment for TNBC can be challenging since the disease has various molecular subtypes. A variety of treatment options are available, such as chemotherapy, immunotherapy, radiotherapy, and surgery. Chemotherapy is the most common of these options. TNBC is generally treated with systemic chemotherapy using drugs such as anthracyclines and taxanes in neoadjuvant or adjuvant settings. Developing resistance to anticancer drugs and off-target toxicity are the primary hindrances to chemotherapeutic solutions for cancer. It is imperative that researchers, clinicians, and pharmaceutical companies work together to develop effective treatment options for TNBC. Several studies have suggested nanotechnology as a potential solution to the problem of suboptimal TNBC treatment. In this review, we summarized possible treatment options for TNBC, including chemotherapy, immunotherapy, targeted therapy, combination therapy, and nanoparticle-based therapy, and some solutions for the treatment of TNBC in the future. Moreover, we gave general information about TNBC in terms of its characteristics and aggressiveness.
Nanoparticle Strategies to Improve the Delivery of Anticancer Drugs across the Blood–Brain Barrier to Treat Brain Tumors
Authors: Vanbilloen Wouter J. F. , Rechberger Julian S. , Anderson Jacob B. , Nonnenbroich Leo F. , Zhang Liang , Daniels David J.
Published: June 2023

Primary brain and central nervous system (CNS) tumors are a diverse group of neoplasms that occur within the brain and spinal cord. Although significant advances in our understanding of the intricate biological underpinnings of CNS neoplasm tumorigenesis and progression have been made, the translation of these discoveries into effective therapies has been stymied by the unique challenges presented by these tumors’ exquisitely sensitive location and the body’s own defense mechanisms (e.g., the brain–CSF barrier and blood–brain barrier), which normally protect the CNS from toxic insult. These barriers effectively prevent the delivery of therapeutics to the site of disease. To overcome these obstacles, new methods for therapeutic delivery are being developed, with one such approach being the utilization of nanoparticles. Here, we will cover the current state of the field with a particular focus on the challenges posed by the BBB, the different nanoparticle classes which are under development for targeted CNS tumor therapeutics delivery, and strategies which have been developed to bypass the BBB and enable effective therapeutics delivery to the site of disease.
Indole Antitumor Agents in Nanotechnology Formulations: An Overview
Authors: Russo Eleonora , Grondona Carola , Brullo Chiara , Spallarossa Andrea , Villa Carla , Tasso Bruno
Published: June 2023

The indole heterocycle represents one of the most important scaffolds in medicinal chemistry and is shared among a number of drugs clinically used in different therapeutic areas. Due to its varied biological activities, high unique chemical properties and significant pharmacological behaviors, indole derivatives have drawn considerable interest in the last decade as antitumor agents active against different types of cancers. The research of novel antiproliferative drugs endowed with enhanced efficacy and reduced toxicity led to the approval by U.S. Food and Drug Administration of the indole-based anticancer agents Sunitinib, Nintedanib, Osimertinib, Panobinostat, Alectinib and Anlotinib. Additionally, new drug delivery systems have been developed to protect the active principle from degradation and to direct the drug to the specific site for clinical use, thus reducing its toxicity. In the present work is an updated review of the recently approved indole-based anti-cancer agents and the nanotechnology systems developed for their delivery.
Study on the Difference of Wavefront Distortion on Beams Caused by Wavelength Differences in Weak Turbulence Region
Authors: Wu Jiali , Ke Xizheng , Kang Weilong , Liang Jingyuan , Ke Chenghu
Published: June 2023

In dual-wavelength free-space optical communication systems, the wavefront distortion differences caused by the wavelength difference of the signal and beacon lights cannot be completely corrected. In this study, according to the method of geometric optics, the relationship between the wavefront phases of the signal and beacon lights is analyzed by establishing a mathematical model of the cross-correlation ratio of the arrival-angle fluctuation and phase relationship of different Gaussian-beam wavelengths under weak turbulence near the surface. The numerical results show that after Gaussian beams of different wavelengths propagate through the same atmospheric channel, the cross-correlation number of the arrival-angle fluctuation decreases with an increase in the wavelength difference, waist radius, and turbulence intensity, and increases with an increase in propagation distance. The beam-wavefront phase difference increases with an increasing wavelength difference. Finally, a wavefront distortion correction experiment of a dual-wavelength adaptive optical system verified the correctness of the wavefront phase-difference relationship between the signal light and beacon light established in this study.
Numerical Simulations of the Decaying Transverse Oscillations in the Cool Jet
Authors: Srivastava Abhishek K. , Singh Balveer
Published: June 2023

In the present paper, we describe a 2.5D (two-and-a-half-dimensional) magnetohydrodynamic (MHD) simulation that provides a detailed picture of the evolution of cool jets triggered by initial vertical velocity perturbations in the solar chromosphere. We implement random multiple velocity, Vy, pulses of amplitude 20–50 km s−1 between 1 Mm and 1.5 Mm in the Sun’s atmosphere below its transition region (TR). These pulses also consist of different switch-off periods between 50 s and 300 s. The applied vertical velocity pulses create a series of magnetoacoustic shocks steepening above the TR. These shocks interact with each other in the inner corona, leading to complex localized velocity fields. The upward propagation of such perturbations creates low-pressure regions behind them, which propel a variety of cool jets and plasma flows in the localized corona. The localized complex velocity fields generate transverse oscillations in some of these jets during their evolution. We study the transverse oscillations of a representative cool jet J1, which moves up to the height of 6.2 Mm above the TR from its origin point. During its evolution, the plasma flows make the spine of jet J1 radially inhomogeneous, which is visible in the density and Alfvén speed smoothly varying across the jet. The highly dense J1, which is triggered along the significantly curved magnetic field lines, supports the propagating transverse wave of period of approximately 195 s with a phase speed of about 125 km s−1. In the distance–time map of density, it is manifested as a transverse kink wave. However, the careful investigation of the distance–time maps of the x- and z-components of velocity reveals that these transverse waves are actually of mixed Alfvénic modes. The transverse wave shows evidence of damping in the jet. We conclude that the cross-field structuring of the density and characteristic Alfvén speed within J1 causes the onset of the resonant conversion and leakage of the wave energy outward to dissipate these transverse oscillations via resonant absorption. The wave energy flux is estimated as approximately of 1.0 × 106 ergs cm−2 s−1. This energy, if it dissipates through the resonant absorption into the corona where the jet is propagated, is sufficient energy for the localized coronal heating.