bluuebunny/crossref_metadata_2025 huggingface dataset:
Spatial Externalities of Income Inequality on Security in Latin America
Authors: Ponce Pablo , Álvarez-García José , Cumbicus Mary , del Río-Rama María de la Cruz
Published: January 2021

The aim of this research is to analyse the effect of income inequality on the homicide rate. The study is carried out in 18 Latin American countries for the period 2005–2018. The methodology used is the Generalized Least Squares (GLS) model and the data were obtained from World Development Indicators, the World Health Organization and the Inter-American Development Bank. Thus, the dependent variable is the homicide rate and the independent variable is income inequality. In addition, some control variables are included, such as: poverty, urban population rate, unemployment, schooling rate, spending on security and GDP per capita, which improve the consistency of the model. The results obtained through GLS model determine that inequality has a negative and significant effect on the homicide rate for high-income countries (HIC) and lower-middle-income countries (LMIC), whereas it is positive and significant for upper-middle-income countries (UMIC). On the other hand, the control variables show different results by group of countries. In the case of unemployment, it is not significant in any group of countries. Negative spatial dependence was found regarding spatial models such as: the spatial lag (SAR) and spatial error (SEM) method. In the spatial Durbin model (SDM), positive spatial dependence between the variables was corroborated. However, spatial auto-regressive moving average (SARMA) identified no spatial dependence. Under these results it is proposed: to improve productivity, education and improve the efficiency of security-oriented resources.
Demographic Dynamics in Multitype Populations with Migrations
Authors: Molina-Fernández Manuel , Mota-Medina Manuel
Published: January 2021

This research work deals with mathematical modeling in complex biological systems in which several types of individuals coexist in various populations. Migratory phenomena among the populations are allowed. We propose a class of mathematical models to describe the demographic dynamics of these type of complex systems. The probability model is defined through a sequence of random matrices in which rows and columns represent the various populations and the several types of individuals, respectively. We prove that this stochastic sequence can be studied under the general setting provided by the multitype branching process theory. Probabilistic properties and limiting results are then established. As application, we present an illustrative example about the population dynamics of biological systems formed by long-lived raptor colonies.
A pde-Based Analysis of the Spectrogram Image for Instantaneous Frequency Estimation
Authors: Bruni Vittoria , Tartaglione Michela , Vitulano Domenico
Published: January 2021

Instantaneous frequency (IF) is a fundamental feature in multicomponent signals analysis and its estimation is required in many practical applications. This goal can be successfully reached for well separated components, while it still is an open problem in case of interfering modes. Most of the methods addressing this issue are parametric, that is, they apply to a specific IF class. Alternative approaches consist of non-parametric time filtering-based procedures, which do not show robustness to destructive interference—the most critical scenario in crossing modes. In this paper, a method for IF curves estimation is proposed. The case of amplitude and frequency modulated two-component signals is addressed by introducing a spectrogram time-frequency evolution law, whose coefficients depend on signal IFs time derivatives, that is, the chirp rates. The problem is then turned into the resolution of a two-dimensional linear system which provides signal chirp rates; IF curves are then obtained by a simple integration. The method is non-parametric and it results quite robust to destructive interference. An estimate of the estimation error, as well as a numerical study concerning method sensitivity and robustness to noise are also provided in the paper.
Design of Constrained Robust Controller for Active Suspension of In-Wheel-Drive Electric Vehicles
Authors: Jin Xianjian , Wang Jiadong , Sun Shaoze , Li Shaohua , Yang Junpeng , Yan Zeyuan
Published: January 2021

This paper presents a constrained robust H∞ controller design of active suspension system for in-wheel-independent-drive electric vehicles considering control constraint and parameter variation. In the active suspension system model, parameter uncertainties of sprung mass are analyzed via linear fraction transformation, and the perturbation bounds can be also limited, then the uncertain quarter-vehicle active suspension model where the in-wheel motor is suspended as a dynamic vibration absorber is built. The constrained robust H∞ feedback controller of the closed-loop active suspension system is designed using the concept of reachable sets and ellipsoids, in which the dynamic tire displacements and the suspension working spaces are constrained, and a comprehensive solution is finally derived from H∞ performance and robust stability. Simulations on frequency responses and road excitations are implemented to verify and evaluate the performance of the designed controller; results show that the active suspension with a developed H∞ controller can effectively achieve better ride comfort and road-holding ability compared with passive suspension despite the existence of control constraints and parameter variations.
Geometric Characterization of Injective Banach Lattices
Authors: Kusraev Anatoly , Kutateladze Semën
Published: January 2021

This is a continuation of the authors’ previous study of the geometric characterizations of the preduals of injective Banach lattices. We seek the properties of the unit ball of a Banach space which make the space isometric or isomorphic to an injective Banach lattice. The study bases on the Boolean valued transfer principle for injective Banach lattices. The latter states that each such lattice serves as an interpretation of an AL-space in an appropriate Boolean valued model of set theory. External identification of the internal Boolean valued properties of the corresponding AL-spaces yields a characterization of injective Banach lattices among Banach spaces and ordered Banach spaces. We also describe the structure of the dual space and present some dual characterization of injective Banach lattices.
Prognostic and Classification of Dynamic Degradation in a Mechanical System Using Variance Gamma Process
Authors: Belhaj Salem Marwa , Fouladirad Mitra , Deloux Estelle
Published: January 2021

Recently, maintaining a complex mechanical system at the appropriate times is considered a significant task for reliability engineers and researchers. Moreover, the development of advanced mechanical systems and the dynamics of the operating environments raises the complexity of a system’s degradation behaviour. In this aspect, an efficient maintenance policy is of great importance, and a better modelling of the operating system’s degradation is essential. In this study, the non-monotonic degradation of a centrifugal pump system operating in the dynamic environment is considered and modelled using variance gamma stochastic process. The covariates are introduced to present the dynamic environmental effects and are modelled using a finite state Markov chain. The degradation of the system in the presence of covariates is modelled and prognostic results are analysed. Two machine learning algorithms k-nearest-neighbour (KNN) and neural network (NN) are applied to identify the various characteristics of degradation and the environmental conditions. A predefined degradation threshold is assigned and used to propose a prognostic result for each classification state. It was observed that this methodology shows promising prognostic results.
Acknowledgment to Reviewers of MCA in 2020
Authors:   MCA Editorial Office
Published: January 2021

Peer review is the driving force of journal development, and reviewers are gatekeepers who ensure that MCA maintains its standards for the high quality of its published papers [...]
Two New Phomaligols from the Marine-Derived Fungus Aspergillus flocculosus and Their Anti-Neuroinflammatory Activity in BV-2 Microglial Cells
Authors: Choi Byeoung-Kyu , Cho Duk-Yeon , Choi Dong-Kug , Trinh Phan Thi Hoai , Shin Hee Jae
Published: January 2021

Two new phomaligols, deketo-phomaligol A (1) and phomaligol E (2), together with six known compounds (3–8) were isolated from the culture broth of the marine-derived fungus Aspergillus flocculosus. Compound 1 was first isolated as a phomaligol derivative possessing a five-membered ring. The structures and absolute configurations of the new phomaligols were determined by detailed analyses of mass spectrometry (MS), nuclear magnetic resonance (NMR) data, optical rotation values and electronic circular dichroism (ECD). In addition, the absolute configurations of the known compounds 3 and 4 were confirmed by chemical oxidation and comparison of optical rotation values. Isolated compounds at a concentration of 100 μM were screened for inhibition of nitric oxide (NO) production in lipopolysaccharide (LPS)-induced BV-2 microglial cells. Among the compounds, 4 showed moderate anti-neuroinflammatory effects with an IC50 value of 56.6 μM by suppressing the production of pro-inflammatory mediators in activated microglial cells without cytotoxicity.
An In-Silico Comparative Study of Lipases from the Antarctic Psychrophilic Ciliate Euplotes focardii and the Mesophilic Congeneric Species Euplotes crassus: Insight into Molecular Cold-Adaptation
Authors: Yang Guang , Mozzicafreddo Matteo , Ballarini Patrizia , Pucciarelli Sandra , Miceli Cristina
Published: January 2021

Cold-adapted enzymes produced by psychrophilic organisms have elevated catalytic activities at low temperatures compared to their mesophilic counterparts. This is largely due to amino acids changes in the protein sequence that often confer increased molecular flexibility in the cold. Comparison of structural changes between psychrophilic and mesophilic enzymes often reveal molecular cold adaptation. In the present study, we performed an in-silico comparative analysis of 104 hydrolytic enzymes belonging to the family of lipases from two evolutionary close marine ciliate species: The Antarctic psychrophilic Euplotes focardii and the mesophilic Euplotes crassus. By applying bioinformatics approaches, we compared amino acid composition and predicted secondary and tertiary structures of these lipases to extract relevant information relative to cold adaptation. Our results not only confirm the importance of several previous recognized amino acid substitutions for cold adaptation, as the preference for small amino acid, but also identify some new factors correlated with the secondary structure possibly responsible for enhanced enzyme activity at low temperatures. This study emphasizes the subtle sequence and structural modifications that may help to transform mesophilic into psychrophilic enzymes for industrial applications by protein engineering.
The Influence of Antiarrhythmic Device Intervention on Biopsychosocial Functioning and Anxiety in Patients with an Implanted Cardioverter Defibrillator
Authors: Karczewska Olimpia , Młynarska Agnieszka
Published: January 2021

Background and objectives: The aim of the study was to assess the impact of cardioverter-defibrillator interventions on the psychosocial functioning of a patient and the occurrence of concerns related to ICD. Materials and Methods: The conducted study was a prospective and observational study that included 158 patients. The study was conducted in two stages: I before ICD implantation and II a follow-up visit six months after the ICD implantation. Standardized questionnaires were used in both stages. Results: In the first six months, a cardioverter-defibrillator discharge occurred in 28 participants, which constituted 17.72% of the study group. The number of ICD discharges positively correlated to insomnia, symptoms of anxiety, symptoms of depression, more discharges, more severe insomnia, anxiety and depression. There was also a negative correlation between the number of discharges and the degree of disease acceptance and in the quality of life domains: somatic, mental, social and environmental. The more discharges, the worse the disease acceptance and assessment of the quality of life. Conclusions: Individuals who experienced discharges assessed their quality of life as worse in all aspects (perception of the quality of life, own health, physical, mental, social and environmental domains), experienced anxiety and depressive disorders more often, were characterized by a worse functioning in a chronic disease, experienced insomnia more often and reported more concerns related to ICD implantation.
Association of Tinnitus with Depression in a Normal Hearing Population
Authors: Choi Jay , Lee Chang Ho , Kim So Young
Published: January 2021

Background and Objectives: The relationship between depression in tinnitus patients without hearing loss remains elusive. This study aimed to investigate the association between tinnitus and normal hearing and depression. Materials and Methods: Participants aged ≥12 years with normal hearing levels were recruited from the Korea National Health and Nutrition Examination Survey (KNHANES), 2009–2012. Participants with normal hearing were divided into the tinnitus and non-tinnitus groups. The relationship between tinnitus with normal hearing and variables including age, sex, depression, ischemic heart diseases, stroke, diabetes, hypertension, dyslipidemia, chronic renal disease, noise exposure, and depression were analyzed. The odds of depression for tinnitus with normal hearing were estimated using multiple logistic regression tests with complex sampling. Results: The results showed that 4.9% (107/2221) and 2.8% (290/10,316) of participants in the tinnitus group and the non-tinnitus group, respectively, experienced depression (p < 0.001). Sex, ischemic heart disease, dyslipidemia, noise exposure, and depression were positively related to tinnitus with normal hearing. The odds ratio of depression for tinnitus with normal hearing were 1.89 (95% CI 1.37–2.60, p < 0.001). Conclusions: Tinnitus with normal hearing was related to the female sex, ischemic heart disease, dyslipidemia, noise exposure, and depression. Depression had the highest odds of tinnitus with normal hearing.
Intelligent Recognition Model of Hot Rolling Strip Edge Defects Based on Deep Learning
Authors: Wang Dongcheng , Xu Yanghuan , Duan Bowei , Wang Yongmei , Song Mingming , Yu Huaxin , Liu Hongmin
Published: January 2021

The edge of a hot rolling strip corresponds to the area where surface defects often occur. The morphologies of several common edge defects are similar to one another, thereby leading to easy error detection. To improve the detection accuracy of edge defects, the authors of this paper first classified the common edge defects and then made a dataset of edge defect images on this basis. Subsequently, edge defect recognition models were established on the basis of LeNet-5, AlexNet, and VggNet-16 by using a convolutional neural network as the core. Through multiple groups of training and recognition experiments, the model’s accuracy and recognition time of a single defect image were analyzed and compared with recognition models with different learning rates and sample batches. The experimental results showed that the recognition model based on the AlexNet had a maximum accuracy of 93.5%, and the average recognition time of a single defect image was 0.0035 s, which could meet the industry requirement. The research results in this paper provide a new method and thought for the fine detection of edge defects in hot rolling strips and have practical significance for improving the surface quality of hot rolling strips.
Tri-Doping of Sol–Gel Synthesized Garnet-Type Oxide Solid-State Electrolyte
Authors: Kim Minji , Kim Gwanhyeon , Lee Heechul
Published: January 2021

The rapidly growing Li-ion battery market has generated considerable demand for Li-ion batteries with improved performance and stability. All-solid-state Li-ion batteries offer promising safety and manufacturing enhancements. Herein, we examine the effect of substitutional doping at three cation sites in garnet-type Li7La3Zr2O12 (LLZO) oxide ceramics produced by a sol–gel synthesis technique with the aim of enhancing the properties of solid-state electrolytes for use in all-solid-state Li-ion batteries. Building on the results of mono-doping experiments with different doping elements and sites—Al, Ga, and Ge at the Li+ site; Rb at the La3+ site; and Ta and Nb at the Zr4+ site—we designed co-doped (Ga, Al, or Rb with Nb) and tri-doped (Ga or Al with Rb and Nb) samples by compositional optimization, and achieved a LLZO ceramic with a pure cubic phase, almost no secondary phase, uniform grain structure, and excellent Li-ion conductivity. The findings extend the current literature on the doping of LLZO ceramics and highlight the potential of the sol–gel method for the production of solid-state electrolytes.
Research on Sapphire Deep Cavity Corrosion and Mask Selection Technology
Authors: Shang Ying-Qi , Zhang Hong-Quan , Zhang Yan
Published: January 2021

Aimed at the problem of the small wet etching depth in sapphire microstructure processing technology, a multilayer composite mask layer is proposed. The thickness of the mask layer is studied, combined with the corrosion rate of different materials on sapphire in the sapphire etching solution, different mask layers are selected for the corrosion test on the sapphire sheet, and then the corrosion experiment is carried out. The results show that at 250 °C, the choice is relatively high when PECVD (Plasma Enhanced Chemical Vapor Deposition) is used to make a double-layer composite film of silicon dioxide and silicon nitride. When the temperature rises to 300 °C, the selection ratio of the silicon dioxide layer grown by PECVD is much greater than that of the silicon nitride layer. Therefore, under high temperature conditions, a certain thickness of silicon dioxide can be used as a mask layer for deep cavity corrosion.
Kinetic Modeling and Degradation Study of Liquid Polysulfide Resin-Clay Nanocomposite
Authors: Shakiba Mohamadreza , Kakoei Arash , Jafari Iman , Rezvani Ghomi Erfan , Kalaee Mohammadreza , Zarei Davood , Abdouss Majid , Shafiei-Navid Saeid , Khosravi Fatemeh , Ramakrishna Seeram
Published: January 2021

Kinetic modeling and degradation study of liquid polysulfide (LPS)/clay nanocomposite is possible through Ozawa–Flynn–Wall (OFW) and Kissinger methods. Comparing the results of these models with experimental data leads to provide an accurate degradation kinetic evaluation of these materials. To this aim, the morphology and distribution of clay nanoparticles (CNPs) within the LPS matrix were investigated using Field Emission Scanning Electron Microscopy (FESEM) and X-ray diffraction (XRD). To evaluate the interaction between the LPS and the CNPs, the Fourier transform infrared (FTIR) identification was utilized. Furthermore, to investigate the kinetics of degradation, the thermal gravimetric analysis (TGA) and derivative thermogravimetry (DTG) of the samples were used in the nitrogen atmosphere with the help of Kissinger and Ozawa–Flynn–Wall (OFW) models. The characterization results confirmed the homogenous dispersion of the CNPs into the LPS matrix. In addition, the presence of CNPs increased the thermal stability and activation energy (Ea) of the samples at different conversion rates. Moreover, the OFW method was highly consistent with the experimental data and provided an appropriate fit for the degradation kinetics.
Caralluma europaea (Guss.) N.E.Br.: Anti-Inflammatory, Antifungal, and Antibacterial Activities against Nosocomial Antibiotic-Resistant Microbes of Chemically Characterized Fractions
Authors: Amrati Fatima Ez-Zahra , Bourhia Mohammed , Saghrouchni Hamza , Slighoua Meryem , Grafov Andriy , Ullah Riaz , Ezzeldin Essam , Mostafa Gamal A. , Bari Ahmed , Ibenmoussa Samir , Bousta Dalila
Published: January 2021

Caralluma europaea (Guss.) N.E.Br.: (C. europaea) is a wild medicinal plant belonging to the family Apocynaceae. It is commonly used in traditional medicines for treating several diseases. The present work aims to evaluate the anti-inflammatory, antibacterial, and antifungal potentials of C. europaea fractions including hydro ethanol (ET CE), n-butanol (But CE), and polyphenol (Poly CE). The chemical composition of hydroethanol, n-butanol, and polyphenol-rich fractions from C. europaea were determined using GC-MS after silylation. The anti-inflammatory effect of hydroethanol, n-butanol, and polyphenol-rich fractions was studied by carrageenan-induced paw edema. Antibacterial and antifungal activities of hydroethanol, n-butanol, and polyphenol-rich fractions against Gram-positive bacteria, Gram-negative bacteria, and yeasts were assessed using the disc diffusion and micro-dilution assays. The findings of the chemical characterization affirmed the presence of interesting bioactive compounds in C. europaea fractions. The polyphenol-rich fraction was the best inhibitor of edema by75.68% after 6 h of treatment. The hydroethanol fraction was the most active against both bacteria and yeasts. This study contributes to society as it provides potential bioactive compounds in C. europaea extract, which may help in fighting nosocomial antibiotic-resistant microbes.
Kahweol, a Diterpenoid Molecule, Inhibits CTGF-Dependent Synthetic Phenotype Switching and Migration in Vascular Smooth Muscle Cells
Authors: Lee Jeong Hee , Choi Seok Tae , Kang Young Jin
Published: January 2021

Vascular smooth muscle cell (VSMC) phenotype switching from contractile to synthetic is essential for proliferation and migration in vascular pathophysiology. Connective tissue growth factor (CTGF) is a matricellular protein involved in cell adhesion, migration, and proliferation. Kahweol, a diterpene molecule in arabica coffee beans, has been reported to have anti-inflammatory, antiproliferative, and apoptotic effects in many cells. However, in VSMCs, the effects of kahweol on CTGF activities have not been investigated. Thus, in this study, the effects and associated mechanisms of kahweol in CTGF-dependent phenotype switching and migration in VSMCs were examined. Experiments were performed on primary rat aortic smooth muscle cells and a rat VSMC line, A7r5. Western blot analysis was used to determine the protein levels. The mRNA levels of synthetic markers were measured by qRT-PCR. Migration of VSMCs was evaluated by wound healing and transwell assays. Kahweol reduced the angiotensin II (Ang II)-induced CTGF expression. Further, kahweol inhibited expressions of synthetic phenotype markers of VSMC. The kahweol-reduced synthetic marker protein levels were reversed by the administration of rCTGF. However, expressions of contractile phenotype markers of VSMC were not affected. Kahweol suppressed Ang II-stimulated VSMC migration. Moreover, kahweol downregulated Ang II-induced p-FAK, p-Erk, and Yes-associated protein (YAP) protein expressions. Taken together, in Ang II-stimulated VSMCs, kahweol inhibited CTGF-dependent synthetic phenotype switching and migration, with focal adhesion kinase (FAK), Erk, and YAP involved in the underlying mechanisms of the kahweol effects. These results suggest that kahweol has a potential as a therapeutic agent to inhibit CTGF, which is a molecular target in sclerogenic vascular disease.
New Photochromic α-Methylchalcones Are Highly Photostable, Even under Singlet Oxygen Conditions: Breaking the α-Methyl Michael-System Reactivity by Reversible Peroxybiradical Formation
Authors: Griesbeck Axel , Öngel Banu , Brüllingen Eric , Renner Melissa
Published: January 2021

The α-methylated chalcones 7a–7e behave as P-type photochromic substances with photo-stationary states (PSS) as high as 15:85 when irradiated at 350 nm. These compounds are easily accessible in pure E-configuration by aldol condensation or by oxidative coupling/elimination. The α-methyl groups make these compounds potentially reactive with singlet oxygen following the gem-rule that predicts 1O2 regioselectivity. Even after long irradiations times in the presence of the singlet oxygen sensitizer tetraphenylporphyrin (TPP) and oxygen, however, no oxygenation products were detected. Under these conditions, all substrates were converted into 9:1 E/Z-mixtures despite the use of low-energy light that does not allow direct or sensitized excitation of the substrates 7. Additionally, chalcone 7a reduced the singlet oxygen reactivity of the tiglic ketone 3a by about a factor of two, indicating substantial physical quenching of singlet oxygen by the α-methylated chalcones 7a–7e. Thus, a singlet oxygen-induced E/Z-isomerization involving 1,2-dioxatetra-methylene biradicals that leads to triplet oxygen and thermodynamic E/Z-mixtures is postulated and supported by quantum chemical (DFT)-calculations.
Binderless, All-Lignin Briquette from Black Liquor Waste: Isolation, Purification, and Characterization
Authors: Mardiyati Yati , Tarigan Emia Yoseva , Prawisudha Pandji , Shoimah Silvia Mar’atus , Rizkiansyah Raden Reza , Steven Steven
Published: January 2021

Lignin isolated from black liquor waste was studied in this research to be utilized as binderless, all-lignin briquette, with a calorific value in the range of 5670–5876 kcal/kg. Isolation of lignin from black liquor was conducted using the acid precipitation method. Sulfuric acid, citric acid, and acetic acid were used to maintain the pH level, which varied from 5 to 2 for the precipitation process. The influence of these isolation conditions on the characteristic of lignin and the properties of the resulted briquette was evaluated through the Klasson method, proximate analysis, ultimate analysis, Fourier Transform Infrared (FTIR), adiabatic bomb calorimeter, density measurement, and Drop Shatter Index (DSI) testing. The finding showed that the lignin isolated using citric acid maintained to pH 3 resulted in briquette with 72% fixed carbon content, excellent 99.7% DSI, and a calorific value equivalent to coal-based briquette.
Evaluation of Dimer of Epicatechin from an Endophytic Fungus Curvularia australiensis FC2AP on Acute Toxicity Levels, Anti-Inflammatory and Anti-Cervical Cancer Activity in Animal Models
Authors: Mani Vellingiri Manon , Soundari Arockiam Jeyasundar Parimala Gnana , Balasubramanian Balamuralikrishnan , Park Sungkwon , Issara Utthapon , Preethi Kathirvel , Liu Wen-Chao
Published: January 2021

Cervical cancer, as the most frequent cancer in women globally and accounts almost 14% in India. It can be prevented or treated with vaccines, radiation, chemotherapy, and brachytherapy. The chemotherapeutic agents cause adverse post effects by the destruction of the neighboring normal cells or altering the properties of the cells. In order to reduce the severity of the side effects caused by the chemically synthesized therapeutic agents, the current research developed an anti-cancer agent dimer of epicatechin (DoE), a natural bioactive secondary metabolite (BSM) mediated from an endophytic fungus Curvularia australiensis FC2AP. The investigation has initiated with the evaluation of inhibiting the angiogenesis which is a main activity in metastasis, and it was assessed through Hen’s Egg Test on Chorio Allantoic Membrane (HET-CAM) test; the BSM inhibited the growth of blood vessels in the developing chick embryo. Further the DoE was evaluated for its acute toxicity levels in albino mice, whereas the survival dose was found to be 1250 mg/kg and the lethal dose was 1500 mg/kg body weight of albino mice; hematological, biochemical, and histopathological analyses were assessed. The anti-inflammatory responses of the DoE were evaluated in carrageenan induced Wistar rats and the reduction of inflammation occurred in a dose-dependent manner. By fixing the effective dose for anti-inflammation analysis, the DoE was taken for the anti-cervical cancer analysis in benzo (a) pyrene induced female Sprague-Dawley rats for 60 days trial. After the stipulated days, the rats were taken for hematological antioxidants, lipid peroxidation (LPO), member bound enzymes, cervical histopathological and carcinogenic markers analyses. The results specified that the DoE has the capability of reducing the tumor in an efficient way. This is the first report of flavonoid-DoE production from an endophytic fungus C. australiensis has the anticancer potentiality and it can be stated as anti-cancer drug.
Root Extracts of Two Cultivars of Paeonia Species: Lipid Composition and Biological Effects on Different Cell Lines: Preliminary Results
Authors: Calonghi Natalia , Farruggia Giovanna , Boga Carla , Micheletti Gabriele , Fini Elena , Romani Lucia , Telese Dario , Faraci Erika , Bergamini Christian , Cerini Stefano , Rizzardi Nicola
Published: January 2021

The roots of two cultivars of Paeonia, namely Paeonia officinalis “Rubra Plena” and Paeonia “Pink Hawaiian Coral”, have been extracted with chloroform. The composition of the lipid fraction, analyzed by GC–MS technique, revealed the absence of paeonol and the presence of phenol, benzoic acid, fatty acid—and some sterol—derivatives. The chloroformic extracts have been tested on normal and several cancer cell lines but showed antiproliferative activity only on the ovarian carcinoma and the osteosarcoma. The biological activity of extracts was investigated mainly by confocal microscopy, flow cytometry and quantum phase imaging. The results indicated that the root extracts induced a hyperpolarization of mitochondria and an increase in reactive oxygen species levels, without inducing cell death. These effects are associated to an increased doubling time and a retarded confluence.
Microwave-Assisted Synthesis of (Piperidin-1-yl)quinolin-3-yl)methylene)hydrazinecarbothioamides as Potent Inhibitors of Cholinesterases: A Biochemical and In Silico Approach
Authors: Munir Rubina , Zia-ur-Rehman Muhammad , Murtaza Shahzad , Zaib Sumera , Javid Noman , Awan Sana Javaid , Iftikhar Kiran , Athar Muhammad Makshoof , Khan Imtiaz
Published: January 2021

Alzheimer’s disease (AD), a progressive neurodegenerative disorder, characterized by central cognitive dysfunction, memory loss, and intellectual decline poses a major public health problem affecting millions of people around the globe. Despite several clinically approved drugs and development of anti-Alzheimer’s heterocyclic structural leads, the treatment of AD requires safer hybrid therapeutics with characteristic structural and biochemical properties. In this endeavor, we herein report a microwave-assisted synthesis of a library of quinoline thiosemicarbazones endowed with a piperidine moiety, achieved via the condensation of 6/8-methyl-2-(piperidin-1-yl)quinoline-3-carbaldehydes and (un)substituted thiosemicarbazides. The target N-heterocyclic products were isolated in excellent yields. The structures of all the synthesized compounds were fully established using readily available spectroscopic techniques (FTIR, 1H- and 13C-NMR). Anti-Alzheimer potential of the synthesized heterocyclic compounds was evaluated using acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) enzymes. The in vitro biochemical assay results revealed several compounds as potent inhibitors of both enzymes. Among them, five compounds exhibited IC50 values less than 20 μM. N-(3-chlorophenyl)-2-((8-methyl-2-(piperidin-1-yl)quinolin-3-yl)methylene)hydrazine carbothioamide emerged as the most potent dual inhibitor of AChE and BChE with IC50 values of 9.68 and 11.59 μM, respectively. Various informative structure–activity relationship (SAR) analyses were also concluded indicating the critical role of substitution pattern on the inhibitory efficacy of the tested derivatives. In vitro results were further validated through molecular docking analysis where interactive behavior of the potent inhibitors within the active pocket of enzymes was established. Quinoline thiosemicarbazones were also tested for their cytotoxicity using MTT assay against HepG2 cells. Among the 26 novel compounds, there were five cytotoxical and 18 showed proliferative properties.
New Substituted 5-Benzylideno-2-Adamantylthiazol[3,2-b][1,2,4]Triazol-6(5H)ones as Possible Anti-Inflammatory Agents
Authors: Tratrat Christophe , Haroun Michelyne , Paparisva Aliki , Kamoutsis Charalmpos , Petrou Anthi , Gavalas Antonis , Eleftheriou Phaedra , Geronikaki Athina , Venugopala Katharigatta N. , Kochkar Hafedh , Nair Anroop B.
Published: January 2021

Background: Inflammation is a complex response to noxious stimuli promoted by the release of chemical mediators from the damaged cells. Metabolic products of arachidonic acid, produced by the action of cyclooxygenase and lipoxygenase, play important roles in this process. Several non-steroidal anti-inflammatory drugs act as cyclooxygenase inhibitors. However, almost all of them have undesired side effects. Methods: Prediction of the anti-inflammatory action of the compounds was performed using PASS Program. The anti-inflammatory activity was evaluated by the carrageenan paw edema test. COX and LOX inhibitory actions were tested using ovine COX-1, human recombinant COX-2 and soybean LOX-1, respectively. Docking analysis was performed using Autodock. Results: All designed derivatives had good prediction results according to PASS and were synthesized and experimentally evaluated. The compounds exhibited in vivo anti-inflammatory action with eleven being equal or better than indomethacin. Although, some of them had no or low inhibitory effect on COX-1/2 or LOX, certain compounds exhibited COX-1 inhibition much higher than naproxen and COX-2 inhibition, well explained by Docking analysis. Conclusions: A number of compounds with good anti-inflammatory action were obtained. Although, some exhibited remarkable COX inhibitory action this activity did not follow the anti-inflammatory results, indicating the implication of other mechanisms.
Ionotropic Gelation of Chitosan Flat Structures and Potential Applications
Authors: Sacco Pasquale , Pedroso-Santana Seidy , Kumar Yogesh , Joly Nicolas , Martin Patrick , Bocchetta Patrizia
Published: January 2021

The capability of some polymers, such as chitosan, to form low cost gels under mild conditions is of great application interest. Ionotropic gelation of chitosan has been used predominantly for the preparation of gel beads for biomedical application. Only in the last few years has the use of this method been extended to the fabrication of chitosan-based flat structures. Herein, after an initial analysis of the major applications of chitosan flat membranes and films and their usual methods of synthesis, the process of ionotropic gelation of chitosan and some recently proposed novel procedures for the synthesis of flat structures are presented.
Recent Advances in the Synthesis of β-Carboline Alkaloids
Authors: Szabó Tímea , Volk Balázs , Milen Mátyás
Published: January 2021

β-Carboline alkaloids are a remarkable family of natural and synthetic indole-containing heterocyclic compounds and they are widely distributed in nature. Recently, these alkaloids have been in the focus of interest, thanks to their diverse biological activities. Their pharmacological activity makes them desirable as sedative, anxiolytic, hypnotic, anticonvulsant, antitumor, antiviral, antiparasitic or antimicrobial drug candidates. The growing potential inherent in them encourages many researchers to address the challenges of the synthesis of natural products containing complex β-carboline frameworks. In this review, we describe the recent developments in the synthesis of β-carboline alkaloids and closely related derivatives through selected examples from the last 5 years. The focus is on the key steps with improved procedures and synthetic approaches. Furthermore the pharmacological potential of the alkaloids is also highlighted.
Optimized Extraction of Amikacin from Murine Whole Blood
Authors: Sardella Roccaldo , Xiroudaki Styliani , Mercolini Laura , Sabbatini Samuele , Monari Claudia , Perito Stefano , Ianni Federica , Vecchiarelli Anna , Giovagnoli Stefano
Published: January 2021

Amikacin (Amk) analysis and quantitation, for pharmacokinetics studies and other types of investigations, is conventionally performed after extraction from plasma. No report exists so far regarding drug extraction from whole blood (WB). This can represent an issue since quantification in plasma does not account for drug partitioning to the blood cell compartment, significantly underrating the drug fraction reaching the blood circulation. In the present work, the optimization of an extraction method of Amk from murine WB has been described. The extraction yield was measured by RP-HPLC-UV after derivatization with 1-fluoro-2,4-dinitrobenzene, which produced an appreciably stable derivative with a favorable UV/vis absorption. Several extraction conditions were tested: spiked Amk disulfate solution/acetonitrile/WB ratio; presence of organic acids and/or ammonium hydroxide and/or ammonium acetate in the extraction mixture; re-dissolution of the supernatant in water after a drying process under vacuum; treatment of the supernatant with a solution of inorganic salts. The use of 5% (by volume) of ammonium hydroxide in a hydro-organic solution with acetonitrile, allowed the almost quantitative (95%) extraction of the drug from WB.
Unraveling the Roles of Vascular Proteins Using Proteomics
Authors: Liu Yan , Lin Tianbao , Valencia Maria Valderrama , Zhang Cankui , Lv Zhiqiang
Published: January 2021

Vascular bundles play important roles in transporting nutrients, growth signals, amino acids, and proteins between aerial and underground tissues. In order to understand these sophisticated processes, a comprehensive analysis of the roles of the components located in the vascular tissues is required. A great deal of data has been obtained from proteomic analyses of vascular tissues in plants, which mainly aim to identify the proteins moving through the vascular tissues. Here, different aspects of the phloem and xylem proteins are reviewed, including their collection methods, and their main biological roles in growth, and biotic and abiotic stress responses. The study of vascular proteomics shows great potential to contribute to our understanding of the biological mechanisms related to development and defense in plants.
Injectable Glycol Chitosan Hydrogel Containing Folic Acid-Functionalized Cyclodextrin-Paclitaxel Complex for Breast Cancer Therapy
Authors: Hyun Hoon , Park Min Ho , Jo Gayoung , Lee Bo Young , Choi Jae Won , Chun Heung Jae , Kim Hyeon Soo , Yang Dae Hyeok
Published: January 2021

We prepared a drug carrier which consisted of injectable methacrylated glycol chitosan (MGC) hydrogel, and a conjugate of 6-monodeoxy-6-monoamino-β-cyclodextrin⋅hydrochloride (6-NH2-β-CD⋅HCl), polyethylene glycol (PEG), and folic acid (FA) for the local delivery and improved cellular uptake of paclitaxel (PTX) (MGC/CDPF-ic-PTX). CDPF refers to a conjugate of 6-NH2-β-CD⋅HCl, PEG, and FA. The anti-cancer effect was investigated using a xenograft mouse model. As controls, the animal study on MGC/PTX and MGC/CD-ic-PTX was performed. The swelling ratio of all samples was analyzed for 7 days, and it showed a gradual increase for 3 days and a maintained state afterward. From the release result, the MGC-based samples have an initial burst for 1 day and a sustained release for 7 days. Results of cytotoxicity and animal study showed the biocompatibility and superior anti-cancer effect of MGC/CDPF-ic-PTX against breast cancer. Furthermore, histological results showed the anti-cancer capacity of MGC/CDPF-ic-PTX against breast cancer. These findings suggest that MGC/CDPF-ic-PTX has clinical potential for breast cancer therapy.
Optimization of Thermophysical and Rheological Properties of Mxene Ionanofluids for Hybrid Solar Photovoltaic/Thermal Systems
Authors: Bakthavatchalam Balaji , Habib Khairul , Saidur R. , Aslfattahi Navid , Yahya Syed Mohd , Rashedi A. , Khanam Taslima
Published: January 2021

Since technology progresses, the need to optimize the thermal system’s heat transfer efficiency is continuously confronted by researchers. A primary constraint in the production of heat transfer fluids needed for ultra-high performance was its intrinsic poor heat transfer properties. MXene, a novel 2D nanoparticle possessing fascinating properties has emerged recently as a potential heat dissipative solute in nanofluids. In this research, 2D MXenes (Ti3C2) are synthesized via chemical etching and blended with a binary solution containing Diethylene Glycol (DEG) and ionic liquid (IL) to formulate stable nanofluids at concentrations of 0.1, 0.2, 0.3 and 0.4 wt%. Furthermore, the effect of different temperatures on the studied liquid’s thermophysical characteristics such as thermal conductivity, density, viscosity, specific heat capacity, thermal stability and the rheological property was experimentally conducted. A computational analysis was performed to evaluate the impact of ionic liquid-based 2D MXene nanofluid (Ti3C2/DEG+IL) in hybrid photovoltaic/thermal (PV/T) systems. A 3D numerical model is developed to evaluate the thermal efficiency, electrical efficiency, heat transfer coefficient, pumping power and temperature distribution. The simulations proved that the studied working fluid in the PV/T system results in an enhancement of thermal efficiency, electrical efficiency and heat transfer coefficient by 78.5%, 18.7% and 6%, respectively.
Effect of Au Nanoparticles and Scattering Layer in Dye-Sensitized Solar Cells Based on Freestanding TiO2 Nanotube Arrays
Authors: Lee Kang-Hun , Han Seung-Hee , Chuquer Ana , Yang Hwa-Young , Kim Jaehi , Pham Xuan-Hung , Yun Won-Ju , Jun Bong-Hyun , Rho Won-Yeop
Published: January 2021

The development of high efficiency dye-sensitized solar cells (DSSCs) has received tremendous attention. Many researchers have introduced new materials for use in DSSCs to achieve high efficiency. In this study, the change in power conversion efficiency (PCE) of DSSCs was investigated by introducing two types of materials—Au nanoparticles (Au NPs) and a scattering layer. A DSSC fabricated without neither Au NPs nor a scattering layer achieved a PCE of 5.85%. The PCE of a DSSC based on freestanding TiO2 nanotube arrays (f-TNTAs) with Au NPs was 6.50% due to better electron generation because the plasmonic absorption band of Au NPs is 530 nm, which matches the dye absorbance. Thus, more electrons were generated at 530 nm, which affected the PCE of the DSSC. The PCE of DSSCs based on f-TNTAs with a scattering layer was 6.61% due to better light harvesting by scattering. The scattering layer reflects all wavelengths of light that improve the light harvesting in the active layer in DSSCs. Finally, the PCE of DSSCs based on the f-TNTAs with Au NPs and a scattering layer was 7.12% due to the synergy of better electron generation and light harvesting by plasmonics and scattering. The application of Au NPs and a scattering layer is a promising research area for DSSCs as they can increase the electron generation and light harvesting ability.
Phase Inversion and Interfacial Layer Microstructure in Emulsions Stabilized by Glycosurfactant Mixtures
Authors: Esposito Rodolfo , Cavasso Domenico , Niccoli Marcella , D’Errico Gerardino
Published: January 2021

Identification of strategies to prolong emulsion kinetic stability is a fundamental challenge for many scientists and technologists. We investigated the relationship between the emulsion stability and the surfactant supramolecular organization at the oil–water interface. The pseudo-phase diagrams of emulsions formed by water and, alternatively, a linear or a branched oil, stabilized by mixtures of two sugar-based surfactants, Span80 and Tween80, are presented. The surfactant ordering and dynamics were analyzed by electron paramagnetic resonance (EPR) spectroscopy. In Oil-in-Water (O/W) emulsions, which are stable for more than four days, disordered surfactant tails formed a compact and viscous layer. In Water-in-Oil (W/O) emulsions, whose stability is much lower, surfactants formed an ordered layer of extended tails pointing toward the continuous apolar medium. If linear oil was used, a narrow range of surfactant mixture composition existed, in which emulsions did not demix in the whole range of water/oil ratio, thus making it possible to study the phase inversion from O/W to W/O structures. While conductometry showed an abrupt inversion occurring at a well-defined water/oil ratio, the surfactant layer microstructure changed gradually between the two limiting situations. Overall, our results demonstrate the interconnection between the emulsion stability and the surfactant layer microstructuring, thus indicating directions for their rational design.
In Vivo Study of Entero- and Hepatotoxicity of Silver Nanoparticles Stabilized with Benzyldimethyl-[3-myristoylamine)-propyl]ammonium Chloride (Miramistin) to CBF1 Mice upon Enteral Administration
Authors: Krutyakov Yurii A. , Kudrinskiy Alexey A. , Kuzmin Vladimir A. , Pyee Jaeho , Gusev Alexander A. , Vasyukova Inna A. , Zakharova Olga V. , Lisichkin Georgy V.
Published: January 2021

Silver nanoparticles (AgNPs) are the most widely studied antimicrobial nanomaterials. However, their use in biomedicine is currently limited due to the availability of data that prove the nanosilver toxicity associated primarily with oxidative stress development in mammalian cells. The surface modification of AgNPs is a potent technique of improvement of their biocompatibility. The synthetic or natural compounds that combine zero or low toxicity towards human and animal organisms with inherent antimicrobial properties are the most promising stabilizing agents, their use would also minimize the risks of microorganisms developing resistance to silver-based materials. We used a simple technique to obtain 30–60 nm AgNPs stabilized with benzyldimethyl[3-myristoylamine)-propyl]ammonium chloride monohydrate (BAC)—a well-known active ingredient of many antibacterial drugs. The objective of the study was to assess the AgNPs-BAC entero- and hepatotoxicity to CBF1 mice upon enteral administration. The animals were exposed to 0.8–7.5 mg/kg doses of AgNPs-BAC in the acute and to 0.05–2.25 mg/kg doses of AgNPs-BAC in the subacute experiments. No significant entero- and hepatotoxic effects following a single exposure to doses smaller than 4 mg/kg were detected. Repeated exposure to the doses of AgNPs-BAC below 0.45 mg/kg and to the doses of BAC below 0.5 mg/kg upon enteral administration also led to no adverse effects. During the acute experiment, the higher AgNPs-BAC dose resulted in increased quantities of aminotransferases and urea, as well as the albumin-globulin ratio shift, which are indicative of inflammatory processes. Besides, the relative mass of the liver of mice was smaller compared to the control. During the subacute experiment, the groups treated with the 0.25–2.25 mg/kg dose of AgNPs-BAC had a lower weight gain rate compared to the control, while the groups treated with the 2.25 mg/kg dose of AgNPs-BAC showed statistically significant variations in the blood serum transaminases activity, which indicated hepatosis. It should be noted that the spleen and liver of the animals from the groups treated with the 0.45 and 2.25 mg/kg dose of AgNPs-BAC were more than two times smaller compared to the control. In the intestines of some animals from the group treated with the 2.25 mg/kg dose of AgNPs-BAC small areas of hyperemia and enlarged Peyer’s patches were observed. Histological examination confirmed the initial stages of the liver and intestinal wall inflammation.