bluuebunny/crossref_metadata_2025 huggingface dataset:
Treatments and Outcomes in Stage I Extranodal Marginal Zone Lymphoma in the United States
Authors: Alderuccio Juan Pablo , Florindez Jorge A. , Reis Isildinha M. , Zhao Wei , Lossos Izidore S.
Published: April 2021

A considerable number of patients with extranodal marginal zone lymphoma (EMZL) are diagnosed with stage I disease. Information on treatments and survival by primary location remains limited. We extracted data from the Surveillance, Epidemiology, and End Results (SEER) database to assess treatment, primary location, and survival of patients with stage I EMZL. Results show that 7961 patients met inclusion criteria. Observation (no treatment) was the most common approach (31%) followed by radiation therapy (RT, 23%). The median overall survival (OS) was 17.3 years (95%CI 16.3 to 18.3). Shorter survival was observed in patients with stage I EMZL compared to expected survival in a cohort derived from the general U.S. population matched by sex, age, and calendar year at diagnosis. However, similar survival was observed in RT-treated patients. We identified age ≥ 60 years (SHR = 4.00, 95%CI 3.10–5.15; p < 0.001), higher grade transformation (SHR = 4.63, 95%CI 3.29–6.52; p < 0.001), and primary lung EMZL (SHR = 1.44, 95%CI 1.05–1.96; p = 0.022) as factors associated with shorter lymphoma-specific survival (LSS). Conversely, primary skin location (SHR = 0.50, 95%CI 0.33–0.77; p = 0.002) was associated with longer LSS. Our results support the use of RT as the preferred approach in localized EMZL.
Evofosfamide Is Effective against Pediatric Aggressive Glioma Cell Lines in Hypoxic Conditions and Potentiates the Effect of Cytotoxic Chemotherapy and Ionizing Radiations
Authors: Bailleul Quentin , Navarin Pauline , Arcicasa Mélanie , Bal-Mahieu Christine , Carcaboso Angel Montero , Le Bourhis Xuefen , Furlan Alessandro , Meignan Samuel , Leblond Pierre
Published: April 2021

Hypoxia is a hallmark of many solid tumors and is associated with resistance to anticancer treatments. Hypoxia-activated prodrugs (HAPs) were developed to target the hypoxic regions of these tumors. Among 2nd generation HAPs, Evofosfamide (Evo, also known as TH-302) exhibits preclinical and clinical activities against adult glioblastoma. In this study, we evaluated its potential in the field of pediatric neuro-oncology. We assessed the efficacy of Evo in vitro as a single drug, or in combination with SN38, doxorubicin, and etoposide, against three pediatric high-grade glioma (pHGG) and three diffuse intrinsic pontine glioma (DIPG) cell lines under hypoxic conditions. We also investigated radio-sensitizing effects using clonogenic assays. Evo inhibited the growth of all cell lines, mainly under hypoxia. We also highlighted a significant synergism between Evo and doxorubicin, SN38, or etoposide. Finally, Evo radio-sensitized the pHGG cell line tested, both with fractionated and single-dose irradiation schedules. Altogether, we report here the first preclinical proof of evidence about Evofosfamide efficiency against hypoxic pHGG and DIPG cells. Since such tumors are highly hypoxic, and Evo potentiates the effect of ionizing radiation and chemotherapy, it appears as a promising therapeutic strategy for children with brain tumors.
Basaltic Outcrops as Centers of Diversity for Xerothermic Plants in the Sudetes Mountains (Central Europe)
Authors: Kwiatkowski Paweł
Published: April 2021

Rock outcrops have promoted a high level of species diversity and provided a stable microclimate for long time periods. The present study is devoted to plant diversity of natural Quaternary outcrops of basaltic rocks. Chorological and ecological investigations were carried out at 35 such outcrops, located within five physiogeographic units of the Sudetes Mountains. The focus was on 120 xerothermic taxa of vascular plants: 62 strictly xerothermic (steppe) taxa of the Festuco valesiacae-Brometea erecti class, and 58 thermophilous taxa representing classes Trifolio medii-Geranietea sanguinei and Quercetea pubescentis. Limited geographical ranges of these plants are manifested by variable frequency of their occurrence. Species distribution is determined by natural factors, like surface area of the outcrop, the type of basaltic rock and the type of plant communities developed. Basaltic outcrops in the Sudetes meet the criterion of habitat islands (inselbergs), serve as regional centers of vascular flora, and are refugia for marginal populations of relict species.
Individualised Timing of Radio-Guided Parathyroidectomy Using Multi-Phase SPECT/CT Increases In Vivo Sensitivity and Accuracy and Reduces Operating Time: A Randomised Clinical Trial
Authors: Formánek Martin , Dedek Vladimír , Koláček Michal , Havel Martin , Zeleník Karol , Komínek Pavel
Published: April 2021

Background: Minimally invasive parathyroidectomy is the preferred treatment for primary hyperparathyroidism. Despite relatively accurate preoperative information, minimally invasive parathyroidectomy can be challenging, especially in the case of small and ectopic adenomas. Radio guidance aids in both in vivo identification and ex vivo confirmation of adenoma. In vivo accuracy is currently not satisfactory. The present study evaluated whether a beneficial effect (increased sensitivity, specificity, accuracy) is obtained with individualised timing of minimally invasive radio-guided parathyroidectomy (MIRGP) using preoperative multi-phase 99mTc-MIBI single photon emission computed tomography (SPECT)/computed tomography (CT). Methods: This randomised clinical trial was conducted from May 2016 to January 2020 in a tertiary referral hospital. Adult patients with primary hyperparathyroidism sent for 99mTc-MIBI SPECT/CT were included consecutively and randomly assigned to conventional (dual-phase) SPECT/CT and conventional MIRGP (group I) or multi-phase SPECT/CT and individualised MIRGP (group II). One hundred of 106 eligible patients were included, and 83 patients underwent complete intervention. Results: A total of 47 patients in group I and 35 patients in group II were analysed. Group II had a shorter operating time (p = 0.003). The in vivo sensitivity and accuracy of radio guidance was 85.1% in group I and 100% in group II (p = 0.046), and 90.4% in group I and 100% in group II (p = 0.021), respectively. We found no difference in the in vivo specificity and ex vivo parameters between groups. Conclusion: Individualised timing increased the in vivo sensitivity and accuracy of radio guidance and reduced operating time, as some parathyroid adenomas rapidly wash out the radionuclide.
Effective Young’s Modulus of Complex Three Dimensional Multilayered Ti/Au Micro-Cantilevers Fabricated by Electrodeposition and the Temperature Dependency
Authors: Watanabe Hitomi , Chang Tso-Fu Mark , Schneider Michael , Schmid Ulrich , Chen Chun-Yi , Iida Shinichi , Yamane Daisuke , Ito Hiroyuki , Machida Katsuyuki , Masu Kazuya , Sone Masato
Published: April 2021

Ti/Au multi-layered micro-cantilevers with complex three-dimensional structures used as micro-components in micro-electromechanical systems (MEMS) sensors were prepared by lithography and electrodeposition, and the effective Young’s modulus was evaluated by the resonance frequency method and finite element method simulation. Effects of the constraint condition at the fixed-end of the micro-cantilever and the temperature dependency of the effective Young’s modulus were studied. Three types of the constraint at the fixed-end were prepared, which were normal type (constraining only bottom surface of the fixed-end), block type (constraining both top and bottom surfaces), and bridge type (top surfaces covering with a bridge-like structure). The temperature dependency test was conducted in a temperature range from 150 to 300 °C in a vacuum chamber. An increase in the effective Young’s modulus was observed as the constraint condition became more rigid, and the effective Young’s modulus merely changed as the temperature varied from room temperature to 300 °C.
Optimum Sensors Allocation for a Forest Fires Monitoring System
Authors: Azevedo Beatriz Flamia , Brito Thadeu , Lima José , Pereira Ana I.
Published: April 2021

Every year forest fires destroy millions of hectares of land worldwide. Detecting forest fire ignition in the early stages is fundamental to avoid forest fires catastrophes. In this approach, Wireless Sensor Network is explored to develop a monitoring system to send alert to authorities when a fire ignition is detected. The study of sensors allocation is essential in this type of monitoring system since its performance is directly related to the position of the sensors, which also defines the coverage region. In this paper, a mathematical model is proposed to solve the sensor allocation problem. This model considers the sensor coverage limitation, the distance, and the forest density interference in the sensor reach. A Genetic Algorithm is implemented to solve the optimisation model and minimise the forest fire hazard. The results obtained are promising since the algorithm could allocate the sensor avoiding overlaps and minimising the total fire hazard value for both regions considered.
Generating New FANCA-Deficient HNSCC Cell Lines by Genomic Editing Recapitulates the Cellular Phenotypes of Fanconi Anemia
Authors: Errazquin Ricardo , Sieiro Esther , Moreno Pilar , Ramirez María José , Lorz Corina , Peral Jorge , Ortiz Jessica , Casado José Antonio , Roman-Rodriguez Francisco J. , Hanenberg Helmut , Río Paula , Surralles Jordi , Segrelles Carmen , Garcia-Escudero Ramon
Published: April 2021

Fanconi anemia (FA) patients have an exacerbated risk of head and neck squamous cell carcinoma (HNSCC). Treatment is challenging as FA patients display enhanced toxicity to standard treatments, including radio/chemotherapy. Therefore, better therapies as well as new disease models are urgently needed. We have used CRISPR/Cas9 editing tools in order to interrupt the human FANCA gene by the generation of insertions/deletions (indels) in exon 4 in two cancer cell lines from sporadic HNSCC having no mutation in FA-genes: CAL27 and CAL33 cells. Our approach allowed efficient editing, subsequent purification of single-cell clones, and Sanger sequencing validation at the edited locus. Clones having frameshift indels in homozygosis did not express FANCA protein and were selected for further analysis. When compared with parental CAL27 and CAL33, FANCA-mutant cell clones displayed a FA-phenotype as they (i) are highly sensitive to DNA interstrand crosslink (ICL) agents such as mitomycin C (MMC) or cisplatin, (ii) do not monoubiquitinate FANCD2 upon MMC treatment and therefore (iii) do not form FANCD2 nuclear foci, and (iv) they display increased chromosome fragility and G2 arrest after diepoxybutane (DEB) treatment. These FANCA-mutant clones display similar growth rates as their parental cells. Interestingly, mutant cells acquire phenotypes associated with more aggressive disease, such as increased migration in wound healing assays. Therefore, CAL27 and CAL33 cells with FANCA mutations are phenocopies of FA-HNSCC cells.
A Link between Replicative Stress, Lamin Proteins, and Inflammation
Authors: Willaume Simon , Rass Emilie , Fontanilla-Ramirez Paula , Moussa Angela , Wanschoor Paul , Bertrand Pascale
Published: April 2021

Double-stranded breaks (DSB), the most toxic DNA lesions, are either a consequence of cellular metabolism, programmed as in during V(D)J recombination, or induced by anti-tumoral therapies or accidental genotoxic exposure. One origin of DSB sources is replicative stress, a major source of genome instability, especially when the integrity of the replication forks is not properly guaranteed. To complete stalled replication, restarting the fork requires complex molecular mechanisms, such as protection, remodeling, and processing. Recently, a link has been made between DNA damage accumulation and inflammation. Indeed, defects in DNA repair or in replication can lead to the release of DNA fragments in the cytosol. The recognition of this self-DNA by DNA sensors leads to the production of inflammatory factors. This beneficial response activating an innate immune response and destruction of cells bearing DNA damage may be considered as a novel part of DNA damage response. However, upon accumulation of DNA damage, a chronic inflammatory cellular microenvironment may lead to inflammatory pathologies, aging, and progression of tumor cells. Progress in understanding the molecular mechanisms of DNA damage repair, replication stress, and cytosolic DNA production would allow to propose new therapeutical strategies against cancer or inflammatory diseases associated with aging. In this review, we describe the mechanisms involved in DSB repair, the replicative stress management, and its consequences. We also focus on new emerging links between key components of the nuclear envelope, the lamins, and DNA repair, management of replicative stress, and inflammation.
Colvis—A Structured Annotation Acquisition System for Data Visualization
Authors: Vanhulst Pierre , Tuor Raphaël , Évéquoz Florian , Lalanne Denis
Published: April 2021

Annotations produced by analysts during the exploration of a data visualization are a precious source of knowledge. Harnessing this knowledge requires a thorough structure of annotations, but also a means to acquire them without harming user engagement. The main contribution of this article is a method, taking the form of an interface, that offers a comprehensive “subject-verb-complement” set of steps for analysts to take annotations, and seamlessly translate these annotations within a prior classification framework. Technical considerations are also an integral part of this study: through a concrete web implementation, we prove the feasibility of our method, but also highlight some of the unresolved challenges that remain to be addressed. After explaining all concepts related to our work, from a literature review to JSON Specifications, we follow by showing two use cases that illustrate how the interface can work in concrete situations. We conclude with a substantial discussion of the limitations, the current state of the method and the upcoming steps for this annotation interface.
On Two-Stage Guessing
Authors: Graczyk Robert , Sason Igal
Published: April 2021

Stationary memoryless sources produce two correlated random sequences Xn and Yn. A guesser seeks to recover Xn in two stages, by first guessing Yn and then Xn. The contributions of this work are twofold: (1) We characterize the least achievable exponential growth rate (in n) of any positive ρ-th moment of the total number of guesses when Yn is obtained by applying a deterministic function f component-wise to Xn. We prove that, depending on f, the least exponential growth rate in the two-stage setup is lower than when guessing Xn directly. We further propose a simple Huffman code-based construction of a function f that is a viable candidate for the minimization of the least exponential growth rate in the two-stage guessing setup. (2) We characterize the least achievable exponential growth rate of the ρ-th moment of the total number of guesses required to recover Xn when Stage 1 need not end with a correct guess of Yn and without assumptions on the stationary memoryless sources producing Xn and Yn.
Metrological Evaluation of Deep-Ocean Thermometers
Authors: Peruzzi Andrea , Bosma Rien , van Geel Jan , Ober Sven
Published: April 2021

An accurate metrological investigation was performed on several units of the two de facto standards for deep-ocean temperature measurements—the SBE35 (the reference thermometer) and the SBE3 (the working thermometer) from SeaBird Scientific. Four SBE35 units were repeatedly calibrated against state-of-the-art fixed-point cells (triple point of water at 0.01 °C and melting point of gallium at 29.7646 °C), with calibration uncertainties of approximately 0.2 mK and 0.5 mK, respectively. Three SBE35 units and one SBE3 unit were calibrated in the temperature range 0 °C to 30 °C, again with sub-millikelvin calibration uncertainties, in a recently-developed water–bath calibration facility. All these calibrations evidenced (1) the deviation of each unit from its original manufacturer’s calibration (up to 1.7 mK), which were found to be inconsistent with the manufacturer’s uncertainty claims and (2) unexplained irreproducibilities, which could not be attributed to the calibration system of up to 1.5 mK. The effect of high pressures (up to 60 MPa) on the response of two SBE35 units was investigated by pressurizing the SBE35 units inside a purpose-built compact pressure enclosure. The results of the pressure investigation confirmed the existence of a small device-dependent pressure effect (approximately 0.3 mK at 60 MPa) and the need for individual temperature–pressure calibration of each SBE35 unit.
Illustrating Important Effects of Second-Order Sensitivities on Response Uncertainties in Reactor Physics
Authors: Cacuci Dan G.
Published: April 2021

This paper illustrates the relative importance of the largest first- and second-order sensitivities of the leakage response of an OECD/NEA reactor physics benchmark (a polyethylene-reflected plutonium sphere) to the benchmark’s underlying total cross sections. It will be shown that numerous 2nd-order sensitivities of the leakage response with respect to the total cross sections are significantly larger than the largest corresponding 1st-order sensitivities. In particular, the contributions of the 2nd-order sensitivities cause the mean (expected) value of the response to differ appreciably from its computed value and also cause the response distribution to be skewed towards positive values relative to the mean. Neglecting these large 2nd-order sensitivities would cause very large non-conservative errors by under-reporting the response’s variance and expected value. The results presented in this paper also underscore the need for obtaining reliable cross section covariance data, which are currently unavailable. Finally, comparing the CPU-times needed for computations, this paper demonstrates that the Second-Order Adjoint Sensitivity Analysis Methodology is the only practical method for computing 2nd-order sensitivities exactly, without introducing methodological errors, for large-scale systems characterized by many uncertain parameters.
Flexible 5-in-1 Microsensor Embedded in the Proton Battery for Real-Time Microscopic Diagnosis
Authors: Lee Chi-Yuan , Chen Chia-Hung , Cheong John-Shong , Chien Yun-Hsiu , Lin Yi-Chuan
Published: April 2021

The proton battery possesses water electrolysis, proton storage and discharging functions simultaneously, and it can be manufactured without expensive metals. Use the principle of proton exchange membrane water electrolysis for charging, store it in the activated carbon on the hydrogen side and use the principle of proton exchange membrane fuel cell for discharge when needed. According to the latest literature, it is difficult to obtain the exact important physical parameters inside the proton battery (e.g., voltage, current, temperature, humidity and flow), and the important physical parameters are correlated with each other, which have critical influence on the performance, lifetime and health status of the proton battery. At present, the condition of the proton battery is judged indirectly only by external measurement, the actual situation inside the proton battery cannot be obtained accurately and instantly. Therefore, this study uses micro-electro-mechanical systems (MEMS) technology to develop a flexible 5-in-1 microsensor, which is embedded in the proton battery to obtain five important physical parameters instantly, so that the condition inside the proton battery can be mastered more precisely, so as to prolong the battery life and enhance the proton battery performance.
Ion Transport in Solid Medium—Evaluation of Ionic Mobility for Design of Ion Transport Pathways in Separator and Gel Electrolyte
Authors: Saito Yuria
Published: April 2021

Further improvement in the performance of lithium secondary batteries will be an indispensable issue to realize a decarbonized society. Among them, the batteries for electric vehicles still have many issues to be addressed because they are subject to various conditions such as high-power performance, safety, and cost restrictions for widespread use. Those subjects require extensive researches from the improvement of each element material to control the battery system to optimize the total performance. Based on this idea, we have been conducting research focusing on ion movement to elucidate the ion conduction mechanism from the microscopic point of view. It has been recognized that the ionic mobility in the battery, which dominates the power performance of the battery, is affected by the solid environment in which the ions move (separator and electrode materials) and the evaluation of ion movement, including the interaction with the surroundings, is necessary as an essential step for battery design. In this article, I will introduce the evaluation approach of ion dynamics and the evaluation results of mobility and interactive situations of carrier ions in the practical separator membranes and gel electrolytes. Finally, the direction of material design is outlined through this review.
Hot Deformation Behavior of a Ni-Based Superalloy with Suppressed Precipitation
Authors: Lizzi Franco , Pradeep Kashyap , Stanojevic Aleksandar , Sommadossi Silvana , Poletti Maria Cecilia
Published: April 2021

Inconel®718 is a well-known nickel-based super-alloy used for high-temperature applications after thermomechanical processes followed by heat treatments. This work describes the evolution of the microstructure and the stresses during hot deformation of a prototype alloy named IN718WP produced by powder metallurgy with similar chemical composition to the matrix of Inconel®718. Compression tests were performed by the thermomechanical simulator Gleeble®3800 in a temperature range from 900 to 1025 °C, and strain rates scaled from 0.001 to 10 s−1. Flow curves of IN718WP showed similar features to those of Inconel®718. The relative stress softening of the IN718WP was comparable to standard alloy Inconel®718 for the highest strain rates. Large stress softening at low strain rates may be related to two phenomena: the fast recrystallization rate, and the coarsening of micropores driven by diffusion. Dynamic recrystallization grade and grain size were quantified using metallography. The recrystallization grade increased as the strain rate decreased, although showed less dependency on the temperature. Dynamic recrystallization occurred after the formation of deformation bands at strain rates above 0.1 s−1 and after the formation of subgrains when deforming at low strain rates. Recrystallized grains had a large number of sigma 3 boundaries, and their percentage increased with strain rate and temperature. The calculated apparent activation energy and strain rate exponent value were similar to those found for Inconel®718 when deforming above the solvus temperature.
Superplasticity at Intermediate Temperatures of ZK60 Magnesium Alloy Processed by Indirect Extrusion
Authors: Palacios-Trujillo César , Victoria-Hernández José , Hernández-Silva David , Letzig Dietmar , García-Bernal Marco A.
Published: April 2021

Magnesium alloys usually exhibit excellent superplasticity at high temperature. However, many Mg alloys have poor formation ability near room temperature. Therefore, preparation of Mg alloys with suitable microstructures to show low or intermediate temperature superplasticity is an important goal. In this work, the superplastic behavior at intermediate temperatures of a commercial ZK60 magnesium alloy processed by indirect extrusion was investigated. After extrusion, the alloy showed a refined and homogeneous microstructure with an average grain size of 4 ± 2 μm. Overall texture measurement indicated that the alloy showed a strong prismatic texture with the highest intensity oriented to pole ⟨101¯0⟩. A texture component ⟨1¯21¯1⟩ parallel to the extrusion direction was found; this type of texture is commonly observed in Mg alloys with rare earth additions. Tensile tests were performed at temperatures of 150, 200, and 250 °C at three strain rates of 10−2, 10−3, and 10−4 s−1. A very high ductility was found at 250 °C and 10−4 s−1, resulting in an elongation to failure of 464%. Based on calculations of the activation energy and on interpretation of the deformation mechanism map for magnesium alloys, it was concluded that grain boundary sliding (GBS) is the dominant deformation mechanism.
An All-Atom Simulation Study of Gas Detonation Forming Technique
Authors: Kulkarni Ambarish , Karkaria Vispi , Nandgaonkar Milankumar , Patil Sandeep P. , Markert Bernd
Published: April 2021

The high-speed forming process is the key to attaining difficult and irregular profiles on ductile materials. In the present work, we proposed the all-atom model of the gas detonation forming process, wherein molecular dynamics (MD) simulations were performed on the aluminum workpiece at different loading speeds similar to the various pressure values in the process. The deformation response of an aluminum workpiece for a wide range of loading speeds, 0.1–8 Å/ps, was investigated. The dome-height, failure patterns, and formability of the aluminum workpiece were examined for these loading speeds. We obtained an inverse relationship between the formability of the aluminum workpiece and the applied loading speed. Moreover, in this work, the influence of the different percentage of defects in the workpieces on the mechanical behavior was investigated. We observed that at lower speeds (< 2 Å/ps), the deformation is observed throughout the workpiece starting from the point of contact in the middle and that is contrary to the deformations observed due to the higher loading speed where localized deformations occur due to creation of slipping planes. We also found that the internal voids lead to the rearrangement of atoms to facilitate the movement of slipping planes leading to better formability compared to the no-void workpieces. This work helps to get a fundamental understanding of deformation behavior in the high-speed forming process with and without defects in the aluminum workpiece at the nanoscale.
Design and Performance Study for Electrothermally Deep-Sea Drive Microunits Using a Paraffin Phase Change Material
Authors: Ning Dayong , Li Zihao , Liang Gangda , Wang Qibo , Zou Weifeng , Gong Yongjun , Hou Jiaoyi
Published: April 2021

Considering the further exploration of the ocean, the requirements for deep-sea operation equipment have increased. Many problems existing in the widely used deep-sea hydraulic system have become increasingly prominent. Compared with the traditional deep-sea hydraulic system, actuators using a paraffin phase change material (PCM) have incomparable advantages, including lightweight structure, low energy consumption, high adaptability to the deep sea, and good biocompatibility. Thus, a deep-sea drive microunit (DDM) based on paraffin PCM is proposed in this paper. The device adopts a flexible shell, adapting to the high-pressure environment of the deep-sea based on the principle of pressure compensation. The device realizes the output of displacement and force through the electrothermal drive, which can be used as actuator or power source of other underwater operation equipment. The microunit successfully completes the functional verification experiments in air, shallow water, and hydrostatic pressure of 110 MPa. In accordance with experimental results, a reasonable control curve is fitted, highlighting its potential application in deep-sea micro electro mechanical systems, especially in underwater soft robot.
Enhanced Resolution Analysis for Water Molecules in MCM-41 and SBA-15 in Low-Field T2 Relaxometric Spectra
Authors: Stoch Grzegorz , Krzyżak Artur T.
Published: April 2021

Mesoporous silica materials are the subjects for relaxometric NMR studies in which we obtain information on the properties of molecules in confined geometries. The signal analysis in such investigations is generally carried out with the help of the Inverse Laplace Transform (ILT), which is accompanied by a regularization procedure. The appropriate selection of the regularization method may positively affect the resolution of the spectrum and the essence of the final conclusions. In this work, we examined the MCM-41 and SBA-15 model systems in various saturation states, using L-Curve regularization for relaxation spectra based on our own version of the fast fast ILT implementation. In a single relaxometric spectrum, the water contributions from the internal volume in the pores and between the silica particles were identified, which allowed us to trace the dynamics of the corresponding drying trends during the removal of water from the sample as a function of total water saturation.
Dynamics of Isomeric and Enantiomeric Fractions of Pinene in Essential Oil of Picea abies Annual Needles during Growing Season
Authors: Kamaitytė-Bukelskienė Liucija , Ložienė Kristina , Labokas Juozas
Published: April 2021

Norway spruce (Picea abies (L.) H. Karst.) is one of the most important commercial tree species distributed naturally in the Boreal and subalpine forest zone of Europe. All parts of spruce trees, including needles, accumulate essential oils with a variety of chemical properties and ecological functions, such as modulating plant–insect communication. Annual needle samples from 15 trees (five from each of three habitats) of 15–17 years old were assayed for essential oils and their major compounds, including α-pinene, β-pinene, (1S)-(−)-α-pinene, and (1R)-(+)-α-pinene across a growing season. Results showed strong positive correlation between percentages of α- and β-pinene isomers (r = 0.69, p < 0.05) and between pinene isomers and essential oils: α-pinene correlated with essential oil stronger (r = 0.62, p < 0.05) than β-pinene (r = 0.33, p < 0.05). Correlation analyses performed with some weather conditions, including average monthly temperature, growing sum of effective temperatures over 5 °C, duration of sunshine, accumulated precipitation, relative humidity, and pressure, showed that temperature is the most important weather condition related to pinene dynamics: negative correlations of moderate strength were established between percentages of α- and β- pinenes and average monthly temperatures (r = −0.36, p < 0.01, n = 75 and r = −0.33, p < 0.01, n = 75, respectively). Out of pinene enantiomers, only (1S)-(−)-α-pinene showed some negative correlation with monthly temperature (r = −0.26, p < 0.05, n = 75). Different patterns of essential oil and pinene dynamics during growing season within separate habitats suggested that some genetic variables of Picea abies might be involved.
Effect of Anode Material on Electrochemical Oxidation of Low Molecular Weight Alcohols—A Review
Authors: Wala Marta , Simka Wojciech
Published: April 2021

The growing climate crisis inspires one of the greatest challenges of the 21st century—developing novel power sources. One of the concepts that offer clean, non-fossil electricity production is fuel cells, especially when the role of fuel is played by simple organic molecules, such as low molecular weight alcohols. The greatest drawback of this technology is the lack of electrocatalytic materials that would enhance reaction kinetics and good stability under process conditions. Currently, electrodes for direct alcohol fuel cells (DAFCs) are mainly based on platinum, which not only provides a poor reaction rate but also readily deactivates because of poisoning by reaction products. Because of these disadvantages, many researchers have focused on developing novel electrode materials with electrocatalytic properties towards the oxidation of simple alcohols, such as methanol, ethanol, ethylene glycol or propanol. This paper presents the development of electrode materials and addresses future challenges that still need to be overcome before direct alcohol fuel cells can be commercialized.
Genomic and Metabolomic Investigation of a Rhizosphere Isolate Streptomyces netropsis WLXQSS-4 Associated with a Traditional Chinese Medicine
Authors: Zhang Songya , Zhang Lingxiao , Zhu Jing , Chen Hong , Chen Zhicong , Si Tong , Liu Tao
Published: April 2021

Rhizosphere microorganisms play important ecological roles in promoting herb growth and producing abundant secondary metabolites. Studies on the rhizosphere microbes of traditional Chinese medicines (TCMs) are limited, especially on the genomic and metabolic levels. In this study, we reported the isolation and characterization of a Steptomyces netropsis WLXQSS-4 strain from the rhizospheric soil of Clematis manshurica Rupr. Genomic sequencing revealed an impressive total of 40 predicted biosynthetic gene clusters (BGCs), whereas metabolomic profiling revealed 13 secondary metabolites under current laboratory conditions. Particularly, medium screening activated the production of alloaureothin, whereas brominated and chlorinated pimprinine derivatives were identified through precursor-directed feeding. Moreover, antiproliferative activities against Hela and A549 cancer cell lines were observed for five compounds, of which two also elicited potent growth inhibition in Enterococcus faecalis and Staphylococcus aureus, respectively. Our results demonstrated the robust secondary metabolism of S. netropsis WLXQSS-4, which may serve as a biocontrol agent upon further investigation.
Effect of Curcumin Addition on the Properties of Biodegradable Pectin/Chitosan Films
Authors: Xie Qingtong , Zheng Xudong , Li Liuting , Ma Liqun , Zhao Qihui , Chang Shiyuan , You Lijun
Published: April 2021

A pectin/chitosan matrix-loaded curcumin film (PCCF) with a deep eutectic solvent (DES) as the solvent and plasticizer was prepared in this study. Different quantities of curcumin (identified as PCCF-0, PCCF-1, PCCF-2. PCCF-3) were loaded on the pectin/chitosan film in order to evaluate their effects on the film properties. Results showed that curcumin could interact with the pectin/chitosan matrix and form a complex three-dimensional network structure. PCCF could promote the thickness, tensile strength, thermal properties, antioxidant and antiseptic capacities, but deteriorate the light transmission and elongation at the same time. The addition of curcumin would change the color of the film, without significantly affecting the moisture content. The tensile strength of PCCF-3 reached the maximum value of 3.75 MPa, while the elongation decreased to 10%. Meanwhile, the water-resistance properties of PCCF-3 were significantly promoted by 8.6% compared with that of PCCF-0. Furthermore, PCCF showed remarkable sustained antioxidant activities in a dose-dependent manner. PCCF-3 could inhibit DPPH and ABTS free radicals by 58.66% and 29.07%, respectively. It also showed antiseptic capacity on fresh pork during storage. Therefore, curcumin addition could improve the barrier, mechanical, antioxidant and antiseptic properties of the polysaccharide-based film and PCCF has the potential to be used as a new kind of food packaging material in the food industry.
Blue Electroluminescence in SRO-HFCVD Films
Authors: Martínez Haydee P. , Luna José A. , Morales Roberto , Casco José F. , Hernández José A. D. , Luna Adan , Hernández Zaira J. , Mendoza Gabriel , Monfil Karim , Ramírez Raquel , Carrillo Jesús , Flores Javier
Published: April 2021

In this work, electroluminescence in Metal-Insulator-Semiconductors (MIS) and Metal-Insulator-Metal (MIM)-type structures was studied. These structures were fabricated with single- and double-layer silicon-rich-oxide (SRO) films by means of Hot Filament Chemical Vapor Deposition (HFCVD), gold and indium tin oxide (ITO) were used on silicon and quartz substrates as a back and front contact, respectively. The thickness, refractive indices, and excess silicon of the SRO films were analyzed. The behavior of the MIS and MIM-type structures and the effects of the pristine current-voltage (I-V) curves with high and low conduction states are presented. The structures exhibit different conduction mechanisms as the Ohmic, Poole–Frenkel, Fowler–Nordheim, and Hopping that contribute to carrier transport in the SRO films. These conduction mechanisms are related to the electroluminescence spectra obtained from the MIS and MIM-like structures with SRO films. The electroluminescence present in these structures has shown bright dots in the low current of 36 uA with a voltage of −20 V to −50 V. However, when applied voltages greater than −67 V with 270 uA, a full area with uniform blue light emission is shown.
Fresh-Water Mollusks as Biomonitors for Ecotoxicity of Nanomaterials
Authors: Abramenko Natalia , Mashkin Petr , Volkov Sergey , Olshanskiy Vladimir , Kustov Leonid
Published: April 2021

The use of different nanoparticles (NPs) is growing every year since discoveries of their unique properties. The wide use of nanomaterials has raised concerns about their safety and possible accumulation in the aquatic environment. Mussels are being considered as one of the most suitable organisms for bioaccumulation monitoring. Within our study, we focused on developing the method that can be applied in field studies of ecotoxicity and can be nondestructive and informative at early times of exposure, while at the same time being based on changes of physiological parameters of fresh water mussels. The changes in the cardiovascular and neural systems of mollusks (Anodonta anatina and Unio tumidus) were measured as biomarkers of toxic effects. Different monometallic and bimetallic NPs, silicon NPs with various ligands were applied as test substances. Changes in cardiovascular and neural functions were in good correlation with accumulation tests for all tested NPs.
Gold-seeded Lithium Niobate Nanoparticles: Influence of Gold Surface Coverage on Second Harmonic Properties
Authors: Taitt Rachael , Urbain Mathias , Behel Zacharie , Pablo-Sainz-Ezquerra Ana-María , Kandybka Iryna , Millet Eloïse , Martinez-Rodriguez Nicolas , Yeromonahos Christelle , Beauquis Sandrine , Le Dantec Ronan , Mugnier Yannick , Brevet Pierre-François , Chevolot Yann , Monnier Virginie
Published: April 2021

Hybrid nanoparticles composed of an efficient nonlinear optical core and a gold shell can enhance and tune the nonlinear optical emission thanks to the plasmonic effect. However the influence of an incomplete gold shell, i.e., isolated gold nano-islands, is still not well studied. Here LiNbO3 (LN) core nanoparticles of 45 nm were coated with various densities of gold nano-seeds (AuSeeds). As both LN and AuSeeds bear negative surface charge, a positively-charged polymer was first coated onto LN. The number of polymer chains per LN was evaluated at 1210 by XPS and confirmed by fluorescence titration. Then, the surface coverage percentage of AuSeeds onto LN was estimated to a maximum of 30% using ICP-AES. The addition of AuSeeds was also accompanied with surface charge reversal, the negative charge increasing with the higher amount of AuSeeds. Finally, the first hyperpolarizability decreased with the increase of AuSeeds density while depolarization values for Au-seeded LN were close to the one of bare LN, showing a predominance of the second harmonic volumic contribution.
3D Bioprinted Scaffolds Containing Mesenchymal Stem/Stromal Lyosecretome: Next Generation Controlled Release Device for Bone Regenerative Medicine
Authors: Bari Elia , Scocozza Franca , Perteghella Sara , Sorlini Marzio , Auricchio Ferdinando , Torre Maria Luisa , Conti Michele
Published: April 2021

Three-dimensional printing of poly(ε-caprolactone) (PCL) is a consolidated scaffold manufacturing technique for bone regenerative medicine. Simultaneously, the mesenchymal stem/stromal cell (MSC) secretome is osteoinductive, promoting scaffold colonization by cells, proliferation, and differentiation. The present paper combines 3D-printed PCL scaffolds with lyosecretome, a freeze-dried formulation of MSC secretome, containing proteins and extracellular vesicles (EVs). We designed a lyosecretome 3D-printed scaffold by two loading strategies: (i) MSC secretome adsorption on 3D-printed scaffold and (ii) coprinting of PCL with an alginate-based hydrogel containing MSC secretome (at two alginate concentrations, i.e., 6% or 10% w/v). A fast release of proteins and EVs (a burst of 75% after 30 min) was observed from scaffolds obtained by absorption loading, while coprinting of PCL and hydrogel, encapsulating lyosecretome, allowed a homogeneous loading of protein and EVs and a controlled slow release. For both loading modes, protein and EV release was governed by diffusion as revealed by the kinetic release study. The secretome’s diffusion is influenced by alginate, its concentration, or its cross-linking modes with protamine due to the higher steric hindrance of the polymer chains. Moreover, it is possible to further slow down protein and EV release by changing the scaffold shape from parallelepiped to cylindrical. In conclusion, it is possible to control the release kinetics of proteins and EVs by changing the composition of the alginate hydrogel, the scaffold’s shape, and hydrogel cross-linking. Such scaffold prototypes for bone regenerative medicine are now available for further testing of safety and efficacy.
Analysis of Fuel Cell Stack Performance Attenuation and Individual Cell Voltage Uniformity Based on the Durability Cycle Condition
Authors: Shen Chunjuan , Xu Sichuan , Gao Yuan
Published: April 2021

Based on the dynamic cycle condition test of a 4.5 kW fuel cell stack, the performance attenuation and individual cell voltage uniformity of the proton exchange membrane fuel cell (PEMFC) stack was evaluated synthetically. The performance decay period of the fuel cell stack was 180–600 h, the decrease of voltage and power was evaluated by rate and amplitude. The results show that the performance of the fuel cell stack decreased with the increase of test time and current density. When the test was carried out to 600 h, under rated operating conditions, the voltage attenuation rate was 130 μV/h, and the voltage reduced by 71 mV, with a decrease of 10.41%. The power attenuation rate was 0.8 W/h, with a decrease of 10.42%. The statistical parameter variation coefficient was used to characterize the voltage consistency of individual cells. It was found that the voltage uniformity is worse at the high current density point and with a long-running process. The variation coefficient was 3.1% in the worst performance.
A Framework of Visual Checkout System Using Convolutional Neural Networks for Bento Buffet
Authors: Wu Mei-Yi , Lee Jia-Hong , Hsueh Chuan-Ying
Published: April 2021

In recent years, the technology of artificial intelligence (AI) and robots is rapidly spreading to countries around the world. More and more scholars and industry experts have proposed AI deep learning models and methods to solve human life problems and improve work efficiency. Modern people’s lives are very busy, which led us to investigate whether the demand for Bento buffet cafeterias has gradually increased in Taiwan. However, when eating at a buffet in a cafeteria, people often encounter two problems. The first problem is that customers need to queue up to check out after they have selected and filled their dishes from the buffet. However, it always takes too much time waiting, especially at lunch or dinner time. The second problem is sometimes customers question the charges calculated by cafeteria staff, claiming they are too expensive at the checkout counter. Therefore, it is necessary to develop an AI-enabled checkout system. The AI-enabled self-checkout system will help the Bento buffet cafeterias reduce long lineups without the need to add additional workers. In this paper, we used computer vision and deep-learning technology to design and implement an AI-enabled checkout system for Bento buffet cafeterias. The prototype contains an angle steel shelf, a Kinect camera, a light source, and a desktop computer. Six baseline convolutional neural networks were applied for comparison on food recognition. In our experiments, there were 22 different food categories in a Bento buffet cafeteria employed. Experimental results show that the inception_v4 model can achieve the highest average validation accuracy of 99.11% on food recognition, but it requires the most training and recognition time. AlexNet model achieves a 94.5% accuracy and requires the least training time and recognition time. We propose a hierarchical approach with two stages to achieve good performance in both the recognition accuracy rate and the required training and recognition time. The approach is designed to perform the first step of identification and the second step of recognizing similar food images, respectively. Experimental results show that the proposed approach can achieve a 96.3% accuracy rate on our test dataset and required very little recognition time for input images. In addition, food volumes could be estimated using the depth images captured by the Kinect camera, and a framework of visual checkout system was successfully built.
Experiment on Activated Carbon Manufactured from Waste Coffee Grounds on the Compressive Strength of Cement Mortars
Authors: Na Sukjoon , Lee Sanghoon , Youn Sungmin
Published: April 2021

In this paper, we performed an experiment with activated carbon manufactured from waste coffee grounds on the compressive strength of normal cement mortars. The activated carbon reinforcement was manufactured from waste coffee grounds, and the collected coffee grounds were then transformed into activated carbon granules through the physical activation process. The activated carbon/cement composites were prepared by mixing cement with activated carbon granules with the weight fractions of 0.5%, 1%, 1.5%, 5%, and 10% cement. The experimental results show that adding activated carbon up to 1.5 wt% increased the early strength of cement mortars. Furthermore, we found that the composites incorporated with a small amount of activated carbon (≤1.5 wt%) had higher compressive strength over the curing period than the normal cement without activated carbon. We believe that these results would potentially have commonalities with morphological symmetry phenomena that occur on the surfaces of activated carbon granules.
Stream Temperature Response to 50% Strip-Thinning in a Temperate Forested Headwater Catchment
Authors: Oanh Dinh Quynh , Gomi Takashi , Moore R. Dan , Chiu Chen-Wei , Hiraoka Marino , Onda Yuichi , Dung Bui Xuan
Published: April 2021

Stream temperature is a critical parameter for understanding hydrological and biological processes in stream ecosystems. Although a large body of research has addressed the effects of forest harvesting on stream temperature, less is known about the responses of stream temperature to the practice of strip-thinning, which produces more coherent patches of shade and sunlight areas. In this study, we examined stream temperature response to 50% strip-thinning in a 17 ha headwater catchment. The thinning lines extended through the riparian zone. Paired-catchment analysis was applied to estimate changes in daily maximum, mean, and minimum stream temperatures for the first year following treatment. Significant effects on daily maximum stream temperature were found for April to August, ranging from 0.6 °C to 3.9 °C, similar to the magnitude of effect found in previous studies involving 50% random thinning. We conducted further analysis to identify the thermal response variability in relation to hydrometeorological drivers. Multiple regression analysis revealed that treatment effects for maximum daily stream temperature were positively related to solar radiation and negatively related to discharge. Frequent precipitation during the summer monsoon season produced moderate increases in discharge (from 1 to 5 mm day−1), mitigating stream temperature increases associated with solar radiation. Catchment hydrologic response to rain events can play an important role in controlling stream thermal response to forest management practices.