bluuebunny/crossref_metadata_2025 huggingface dataset:
A first glance into the black box of life satisfaction surrounding childbearing
Authors: Aassve Arnstein , Luppi Francesca , Mencarini Letizia
Published: June 2021

The vast majority of studies looking into the relationship between childbearing and subjective well-being use overall measures where respondents either report their general level of happiness or their life satisfaction, leaving substantial doubt about the underlying mechanisms. However, life satisfaction and happiness are intuitively multidimensional concepts, simply because there cannot be only one aspect that affects individuals' well-being. In this study, by considering seventeen specific life satisfaction domains, these features come out very clearly. Whereas all the domains considered matter for the overall life satisfaction, only three of them, namely satisfaction with leisure, health and satisfaction with the partnership, change dramatically surrounding childbearing events. Even though we cannot generalise (since these results stem from one particular panel survey, i.e., Household Income and Labour Dynamics in Australia data), it appears that the typical anticipation and post-child decrease of life satisfaction, so often found in existing studies, stems from changes in these three domains.
Dipeptidyl peptidase-IV inhibitory peptides derived from salmon milt and their effects on postprandial blood glucose level
Authors: Takahashi Yoshinori , Kamata Akira , Konishi Tatsuya
Published: June 2021

The hydrolysate of Oncorhynchus keta (chum salmon) milt was found to exhibit strong inhibitory activity against dipeptidyl peptidase-IV (DPP-IV), and the hypoglycaemic effect of these salmon milt peptides (SMPs) was confirmed using oral starch tolerance tests in Sprague–Dawley rats. Specifically, the oral administration of SMPs at a dose of 300 mg/kg body weight for 1 week resulted in a significant decrease in the blood glucose level of the rats 60 min after starch consumption compared with the control. The bioassay-guided separation of SMPs, using a combination of column chromatographic techniques, led to the identification of 12 active di-, tri- and tetrapeptides, including four novel DPP-IV inhibitory peptides, namely Phe-Pro-Val-Gly, Leu-Pro-Val-Leu, Val-Pro-Phe-Pro and Ile-Pro-Leu. Among these 12 peptides, Ile-Pro contributed the most (1.3%) to the total DPP-IV inhibitory activity of SMPs. Therefore, SMPs may have the potential to be used as a suppressor of postprandial hyperglycaemia and to be considered a promising functional food material for the prevention of type II diabetes.
Correction to: A solution for funding the development of technology in oilwell drilling
Authors: Amorim Dalmo S. , Santos Otto L. A. , Azevedo Ricardo C. , Chieregati Ana Carolina
Published: June 2021

The word “templated” has been corrected to “tempted”.
A non-empirical model for gas transfer through circular nanopores in unconventional gas reservoirs
Authors: Li Jiangtao , Wei Jianguang , Ji Liang , Wang Anlun , Rong Gen , Li Jiyao , Chen Lin , Sun Zheng
Published: June 2021

It is difficult to predict the flow performance in the nanopore networks since traditional assumptions of Navier–Stokes equation break down. At present, lots of attempts have been employed to address the proposition. In this work, the advantages and disadvantages of previous analytical models are seriously analyzed. The first type is modifying a mature equation which is proposed for a specified flow regime and adapted to wider application scope. Thus, the first-type models inevitably require empirical coefficients. The second type is weight superposition based on two different flow mechanisms, which is considered as the reasonable establishment method for universal non-empirical gas-transport model. Subsequently, in terms of slip flow and Knudsen diffusion, the novel gas-transport model is established in this work. Notably, the weight factors of slip flow and Knudsen diffusion are determined through Wu’s model and Knudsen’s model respectively, with the capacity to capture key transport mechanism through nanopores. Capturing gas flow physics at nanoscale allows the proposed model free of any empirical coefficients, which is also the main distinction between our work and previous research. Reliability of proposed model is verified by published molecular simulation results as well. Furthermore, a novel permeability model for coal/shale matrix is developed based on the non-empirical gas-transport model. Results show that (a) nanoconfined gas-transport capacity will be strengthened with the decline of pressure and the decrease in the pressure is supportive for the increasing amplitude; (b) the greater pore size the nanopores is, the stronger the transport capacity the nanotube is; (c) after field application with an actual well in Fuling shale gas field, China, it is demonstrated that numerical simulation coupled with the proposed permeability model can achieve better historical match with the actual production performance. The investigation will contribute to the understanding of nanoconfined gas flow behavior and lay the theoretical foundation for next-generation numerical simulation of unconventional gas reservoirs.
Construction of pore structure and lithology of digital rock physics based on laboratory experiments
Authors: Huang Chenzhi , Zhang Xingde , Liu Shuang , Li Nianyin , Kang Jia , Xiong Gang
Published: June 2021

The development and stimulation of oil and gas fields are inseparable from the experimental analysis of reservoir rocks. Large number of experiments, poor reservoir properties and thin reservoir thickness will lead to insufficient number of cores, which restricts the experimental evaluation effect of cores. Digital rock physics (DRP) can solve these problems well. This paper presents a rapid, simple, and practical method to establish the pore structure and lithology of DRP based on laboratory experiments. First, a core is scanned by computed tomography (CT) scanning technology, and filtering back-projection reconstruction method is used to test the core visualization. Subsequently, three-dimensional median filtering technology is used to eliminate noise signals after scanning, and the maximum interclass variance method is used to segment the rock skeleton and pore. Based on X-ray diffraction technology, the distribution of minerals in the rock core is studied by combining the processed CT scan data. The core pore size distribution is analyzed by the mercury intrusion method, and the core pore size distribution with spatial correlation is constructed by the kriging interpolation method. Based on the analysis of the core particle-size distribution by the screening method, the shape of the rock particle is assumed to be a more practical irregular polyhedron; considering this shape and the mineral distribution, the DRP pore structure and lithology are finally established. The DRP porosity calculated by MATLAB software is 32.4%, and the core porosity measured in a nuclear magnetic resonance experiment is 29.9%; thus, the accuracy of the model is validated. Further, the method of simulating the process of physical and chemical changes by using the digital core is proposed for further study.
A study on cashew nut shell liquid as a bio-based flow improver for heavy crude oil
Authors: Pandian Sivakumar , Dahyalal Patel Chintan , Krishna Shanker , Hari S. , Subramanian Deepalakshmi
Published: June 2021

Transportation of heavy crude oil through pipelines poses a great challenge in oil and gas industry. Crude oil chokes the pipelines when the temperature drops below the pour-point temperature. In the present study, a bio-based additive, i.e., Cashew Nut Shell Liquid (CNSL) has been tested as a flow improver for heavy crude. CNSL was obtained from waste cashew nut shell by means of mechanical extraction, and it was completely characterized. Similarly, the crude oil used in the study was characterized for its physio-chemical properties. Also, the crude oil was subjected to Saturates, Aromatics, Resins and Asphaltene analysis and Fourier Transform Infra-Red analysis. The raw and additive-treated crude oil with different CNSL dosages were subjected to pour-point and rheology measurements and optical micro-imaging analysis which indicated a remarkable improvement in flow whereby an optimum dose of 2000 ppm was observed. Furthermore, the effects of different parameters like shear rate, concentration of the flow improver and the effect of temperature on the crude oil flowability were studied. The process variables were optimized by means of Taguchi method, and the percentage contribution of each parameter was identified with the help of ANOVA table. The results indicate that a remarkable improvement in flow was observed at an optimum dose of 2000 ppm. The contribution of the concentration was found to be around 53%, whereas the contributions of the shear rate and the temperature were only 18.08 and 28.91%, respectively. Therefore, it has been observed that CNSL flow improvers extracted from cheap reasonable resources are more effective as they are cost-effective and eco-friendly when compared to conventional additives.
A predictive score for 30-day survival for patients undergoing major lower limb amputation for peripheral arterial obstructive disease
Authors: Franchin Marco , Palermo Vincenzo , Iannuzzi Carlo , Rivolta Nicola , Mozzetta Gaddiel , Tozzi Matteo , Bush Ruth L. , Piffaretti Gabriele
Published: June 2021

To analyze outcomes following major lower extremity amputations (mLEAs) for peripheral arterial obstructive disease, gangrene, infected non-healing wound and to create a risk prediction scoring system for 30-day mortality. In this single-center, retrospective, observational cohort study. All patients treated with above-the-knee amputation (AKA) or below-the-knee amputation (BKA) between January 1st, 2010 and June 30th, 2018 were identified. The primary outcome of interest was early (≤ 30 days) mortality. Secondary outcomes were postoperative complications and freedom from amputation stump revision/failure. We identified 310 (77.7%) mLEAs performed on 286 patients. There were 188 (65.7%) men and 98 (34.3%) women with a median age of 79 years (IQR, 69–83 years). We performed 257 (82.9%) AKA and 53 (17.1%) BKA. There were 49 (15.8%) early deaths, which did not differ among the age quartiles of this cohort (15.4% vs. 14.3% vs. 15.4% vs. 19.5%, P = 0.826). Binary logistic regression analysis identified age > 80 years (OR 2.24, 95% CI 1.17–4.31; P = 0.015), chronic obstructive pulmonary disease (OR 2.12, 95% CI 1.11–4.06; P = 0.023), and hemodialysis (OR 2.52, 95% CI 1.15–5.52; P = 0.021) to be associated with early mortality. The final score (range 0–10) identified two subgroups with different mortality at 30 days: lower-risk (score < 4, 10.8%), and higher-risk (score ≥ 4: 28.7%; OR 3.2, 95% CI 1.63–6.32; P < 0.001). In our experience, mLEAs still have a 14% mortality rate over the years. Our lower-risk group (score < 4) is characterized by a lower rate of perioperative death and longer survival.

Graphic abstract
Jain point: an alternate laparoscopic non-umbilical first blind entry port to avoid vessel, viscera, adhesions and bowel (VVAB)
Authors: Jain Nutan , Jain Vandana , Chandi Anadeep , Srivastava Sakshi , Singh Shalini , Vasundhara N.
Published: June 2021

The Jain point entry is based on the concept of non-umbilical entry to avoid sudden catastrophic injury to major retroperitoneal vessels, viscera, adhesions and bowel which could happen before the start of procedure by blind umbilical entry. To study the safety and efficacy of a novel first non-umbilical blind entry port. Tertiary referral centre for advanced laparoscopic surgeries with active training and fellowship programs. A large retrospective study of 7802 cases done at Vardhman Infertility & Laparoscopy Centre from January 2011 to December 2020. In all cases, first blind entry was by veress needle and 5 mm trocar and telescope through a non-umbilical port, The Jain point, irrespective of BMI, large masses, lax abdomen, previous surgery and complex situations. Patients’ demographic profile, types of surgeries performed and entry-related complications were recorded and analysed. Mean age of patients was 33 years with BMI ranging from 12.66 to 54.41 kg/m2. Thus, Jain point can be applicable for all ranges of BMI, all types of surgeries from simple to complex and large masses. Entry related minor complications were in 3.4% cases while major complication involving bowel occurred in one case. No case of injury to major retro-peritoneal vessel was seen. Jain point entry is a novel, first blind 5 mm non-umbilical, entry technique in a variety of surgeries and previous scars and patients with wide range of BMI. It has a short learning curve and continues as main ergonomic working port.
Multi-criteria modelling of drought: a study of Brandenburg Federal State, Germany
Authors: Ihinegbu Christopher , Ogunwumi Taiwo
Published: June 2021

Drought is the absence or below-required supply of precipitation, runoff and or moisture for an extended time period. Modelling drought is relevant in assessing drought incidence and pattern. This study aimed to model the spatial variation and incidence of the 2018 drought in Brandenburg using GIS and remote sensing. To achieve this, we employed a Multi-Criteria Approach (MCA) by using three parameters including Precipitation, Land Surface Temperature and Normalized Difference Vegetation Index (NDVI). We acquired the precipitation data from Deutsche Wetterdienst, Land Surface Temperature and NDVI from Landsat 8 imageries on the USGS Earth Explorer. The datasets were analyzed using ArcGIS 10.7. The information from these three datasets was used as parameters in assessing drought prevalence using the MCA. The MCA was used in developing the drought model, ‘PLAN’, which was used to classify the study area into three levels/zones of drought prevalence: moderate, high and extreme drought. We went further to quantify the agricultural areas affected by drought in the study area by integrating the land use map. Results revealed that 92% of the study area was severely and highly affected by drought especially in districts of Oberhavel, Uckermark, Potsdam-Staedte, and Teltow-Flaeming. Finding also revealed that 77.54% of the total agricultural land falls within the high drought zones. We advocated for the application of drought models (such as ‘PLAN’), that incorporates flexibility (tailoring to study needs) and multi-criteria (robustness) in drought assessment. We also suggested that adaptive drought management should be championed using drought prevalence mapping.
Pore structure and sorption capacity investigations of Ediacaran and Lower Silurian gas shales from the Upper Yangtze platform, China
Authors: Hu Zhazha , Gaus Garri , Seemann Timo , Zhang Qian , Littke Ralf , Fink Reinhard
Published: June 2021

The shale gas potential of Ediacaran and Lower Silurian shales from the Upper Yangtze platform is assessed in this study with a focus on the contributions of clay minerals and organic matter to sorption capacity. For this purpose, a multidisciplinary assessment was carried out using petrophysical, mineralogical, petrographic and geochemical methods. In terms of TOC contents (4.2%), brittle mineral contents (68.6%) and maximum gas storage capacities (0.054–0.251 mmol/g) Ediacaran shales from this study show comparable properties to other producing shale gas systems although the thermal maturity is extremely high (VRr = 3.6%). When compared to lower Silurian shales from the same region, it is evident that (1) deeper maximum burial and (2) a lack of silica-associated preservation of the pores resulted in a relatively lower mesopore volume, higher micropore volume fraction and lower overall porosity (Ediacaran shales: 1.4–4.6%; Silurian shales: 6.2–7.4%). Gas production is therefore retarded by poor interconnectivity of the pore system, which was qualitatively demonstrated by comparing experimental gas uptake kinetics. TOC content exhibits a prominent control on sorption capacity and micropore volume for both shales. However, different contributions of clay minerals to sorption capacity were identified. This can partly be attributed to different clay types but is likely also related to burial-induced recrystallisation and different origins of illite. Additionally, it was shown that variations in sorption capacity due to incorrect estimates of clay mineral contribution are in the same range as variations due to differences in thermal maturity.

Article highlights

Pore structure and gas storage characteristics are evaluated for the first time for Ediacaran Shales from the Upper Yangtze platform

Due to a lower free gas storage capacity and diffusivity, the Ediacaran shale can be regarded as a less favorable shale gas prospect when compared to the Silurian shale

Clay mineral contribution to sorption capacity is evaluated taking clay mineralogy into consideration

Maturity-related changes of organic matter sorption capacity have been discussed on the basis of a compiled data set
Structural and thermal properties of pure and chromium doped zinc oxide nanoparticles
Authors: Worku Ababay Ketema , Ayele Delele Worku , Habtu Nigus Gabbiye , Melas Getu Alemayehu , Yemata Temesgen Atnafu , Mekonnen Negese Yayu , Teshager Minbale Admas
Published: June 2021

Pure ZnO and Cr-doped ZnO nanoparticles have been synthesized via a facile chemical co-precipitation route and their structural, thermal characteristics were discussed systematically. In the experimental producer, the doping concentration has varied the range, 0.05–0.1 M, while calcined at 600 °C. The influence of Cr-doping on the physical characteristics of ZnO nanoparticles was investigated and addressed. As-prepared samples were analyzed via XRD, FTIR, TGA/DTA, BET, and ICP-MS. XRD analysis shows that ZnO and Cr doped ZnO nanoparticles with average particle sizes between 23 and 39 nm were successfully developed with hexagonal wurtzite structure. The FTIR spectroscopy analysis confirms the existence of chromium in the doped ZnO nanoparticles and the formation of ZnO. The TGA/DTA analysis shows that Cr–ZnO nanoparticles are more thermally stable than ZnO nanoparticles. Moreover, the dopant concentration has been analyzed via ICP-MS and showed a good agreement with the expected chromium concentration. The BET surface area measurement shows that 176.25 m2/g and 287.17 m2/g for un-doped ZnO, and 0.1 M Cr-doped ZnO nanoparticles, respectively. Hence, doping of Cr enhances the surface area and thermal stability. Thus, Cr–ZnO nanoparticles show good thermal stability, and high surface area, which is an excellent characteristices of nanomaterials.

Graphic abstract
International Human Rights Law Beyond State Territorial Control
Authors: Berkes Antal
Published: June 2021

Can international human rights law be applied and enforced in a part of a State's territory outside its effective control? This study provides a step by step analysis to show how it can. International human rights law can normalise an imperfect, defective situation through pragmatic interpretation; it imposes obligations both on the territorial State on account of its sovereign title and residual effectiveness on the one hand, and on any subject of international law exercising territorial control over the area on account of its effective control on the other. By considering effectiveness beyond formal normative sources and titles of the subjects implicated in the territorial situation, international human rights law is interpreted and applied in a manner which renders human rights practical and effective. The book provides a comprehensive analysis of State practice regarding various subjects implicated in the territorial situation, applicable legal sources and major geographic areas.
Sea surface temperature seasonality in the northern South China Sea during the middle Holocene derived from high resolution Sr/Ca ratios of Tridacna shells
Authors: Zhou Pengchao , Yan Hong , Shi Ge , Liu Chengcheng , Luo Fan , Han Tao , Wang Guozhen , Wen Hanfeng , Zhao Nanyu , Dodson John , Li Yue , Zhou Weijian
Published: June 2021

Seasonal climate variability is an important component of Earth's climate system, and has a significant impact on ecosystems and social systems. However, the temporal resolution of most proxy-based paleoclimate records is limiting to fully understand the past seasonal changes. Here, we used high-precision monthly resolution Sr/Ca records of three Tridacna squamosa specimens from the northern South China Sea (SCS) to reconstruct the sea surface temperature (SST) seasonality during three time periods from the middle Holocene. The results suggested that SST seasonality in the northern SCS during the middle Holocene (3.21 ± 0.98°C) was smaller than that for recent decades (AD 1994–2004, 4.32 ± 0.59°C). Analysis of modern instrumental data showed that the SST seasonality in the northern SCS was dominated by the winter SST, which was deeply influenced by the intensity of East Asian winter monsoon (EAWM). A strong EAWM usually resulted in cooler winter SST and a larger SST seasonality in the northern SCS. The reconstructed Holocene EAWM records showed that the EAWM strengthened from the middle to late Holocene, which was seen in our reconstruction of less SST seasonality changes during the middle Holocene in the northern SCS. This study highlighted that the Sr/Ca ratios from Tridacna shells can be used as a potential high-resolution indicator of past seasonal climate changes.
Generalized manifolds, normal invariants, and 𝕃-homology
Authors: Hegenbarth Friedrich , Repovš Dušan
Published: June 2021

Let $X^{n}$ be an oriented closed generalized $n$-manifold, $n\ge 5$. In our recent paper (Proc. Edinb. Math. Soc. (2) 63 (2020), no. 2, 597–607), we have constructed a map $t:\mathcal {N}(X^{n}) \to H^{st}_{n} ( X^{n}; \mathbb{L}^{+})$ which extends the normal invariant map for the case when $X^{n}$ is a topological $n$-manifold. Here, $\mathcal {N}(X^{n})$ denotes the set of all normal bordism classes of degree one normal maps $(f,\,b): M^{n} \to X^{n},$ and $H^{st}_{*} ( X^{n}; \mathbb{E})$ denotes the Steenrod homology of the spectrum $\mathbb{E}$. An important non-trivial question arose whether the map $t$ is bijective (note that this holds in the case when $X^{n}$ is a topological $n$-manifold). It is the purpose of this paper to prove that the answer to this question is affirmative.
Computational efficiency of multi-body systems dynamic models
Authors: Antonya Cs. , Boboc R. G.
Published: June 2021

For several decades, simulation and analysis of mechanisms have been performed with dedicated computer-aided engineering software that implements general dynamic formulations, known in the literature as multi-body systems (MBS) formulations. The MBS name is related to the structure of the mechanism, which is often considered to be a collection of bodies interconnected by mechanical joints (pairs). Nevertheless, only a few formulations are really based on a true multi-body mechanical model, while many others instantiate mathematically quite different mechanical concepts. This paper aims to identify and discuss the mechanical models that fundament the main multi-body mathematical formulations known in the literature. The main features of each model are outlined, based on a detailed presentation of the structure and equations of motion, together with their link with the kinematic and dynamic formulations. A comparative study related to computational efficiency is then presented for the identified main models, based on a test mechanism. Comparative advantages and disadvantages are discussed at the end, considering the identified mechanical models.
Un orologio solare miniaturistico in avorio da un contesto residenziale tardo-repubblicano di Pisa
Authors: Taccola Emanuele , Bonnin Jérôme , Savoie Denis , Battistoni Filippo
Published: June 2021

L'oggetto che si presenta in questo contributo costituisce un'integrazione al corpus relativamente poco cospicuo di orologi solari miniaturistici greco-romani. Si tratta di una meridiana conica in avorio di elefante, rinvenuta in giacitura secondaria all'interno di un contesto residenziale di età augustea nella Piazza del Duomo di Pisa. Al di là dell'unicità dell'esemplare, per il quale si fornisce un accurato studio gnomonico, unitamente a un approfondito apprezzamento dei relativi aspetti culturali, sociali e tipologici, tale scoperta contribuisce a meglio definire il livello sociale di quest'area della città tra il II e il I sec. a.C.
On the Estimation of the Ratio of ULF Wave Electric Fields in Space and the Magnetic Fields at the Ground
Authors: Warden L. J. , Waters C. L. , Sciffer M. D. , Hull A. J.
Published: June 2021

Three new methods for estimating a ratio of the ultralow frequency (ULF; 1–100 mHz) wave equatorial electric field amplitude in the Earth's magnetosphere to ground magnetic field amplitudes for field line resonances (FLR) are described. These methods use ratios of the time series extrema, ratios of the envelope waveform and the ratio of the spectral amplitude at the FLR frequency. These methods were applied to four ULF resonance intervals; three detected by the Van Allen Probe A spacecraft and one detected by the POLAR spacecraft. The intervals were conjoined with the CARISMA and IMAGE ground magnetometer arrays. The spectral ratio results for the Van Allen Probe intervals were approximately twice to three times the ratios estimated from the two time series based methods. The POLAR interval showed similar values across all three methods. The differences are attributed to broadband frequency signals that modify the time series amplitudes, while the spectral method avoids these off‐resonant frequencies. Based on the results of this study, a spectral based method for calculating the ratio at the FLR frequency is best.
Assessment of Quartz Grain Growth and the Application of the Wattmeter to Predict Quartz Recrystallized Grain Sizes
Authors: Tokle Leif , Hirth Greg
Published: June 2021

Relationships between the recrystallized grain size and stress are investigated for experimentally deformed water‐added quartz aggregates. For stresses ≥100 MPa there is a variation in the measured recrystallized grain size for a given stress. This variation correlates with a change in the c‐axis fabric in general shear experiments, where samples with larger recrystallized grain sizes for a given stress have dominantly prism  c‐axis fabrics and samples with smaller recrystallized grain sizes for a given stress have dominantly basal  c‐axis fabrics. The dislocation creep flow law also changes at conditions where these two c‐axis fabrics form (Tokle et al., 2019, https://doi.org/10.1016/j.epsl.2018.10.017). Using the wattmeter model (Austin & Evans, 2007, https://doi.org/10.1130/G23244A.1), different piezometric relationships are quantified for samples that develop prism  and basal  c‐axis fabrics, respectively. The wattmeter model is sensitive to grain growth kinetics; a new grain growth law for quartz is formulated based on reanalysis of microstructures in samples from previous work. The activation enthalpies and water fugacity exponents for our grain growth law and dislocation creep flow laws are the same within error, suggesting the recrystallized grain size versus stress relationships are nearly independent of temperature and water fugacity, consistent with laboratory observations. The wattmeters successfully predict the recrystallized grain size versus stress relationships of all quartzite samples from experiments with added water. These results support the use and extrapolation of the wattmeter model for both experimental and geologic conditions to investigate the stress state and grain size evolution of quartz rich rocks.
Effects of Atmospheric Circulation on Stream Chemistry in Forested Watersheds Across the Northeastern United States: Part 2. Interannual Weather Type Variability
Authors: Suriano Z. J. , Siegert C. M. , Leathers D. J. , Gold A. J. , Addy K. , Schroth A. W. , Seybold E. , Inamdar S. , Levia D. F.
Published: June 2021

Using synoptic classification techniques, synoptic‐scale weather types associated with large exports of dissolved organic carbon and nitrate‐nitrogen in three forested watersheds in the northeastern United States are analyzed. In contrast to Siegert et al. (2021,https://doi.org/10.1029/2020jd033413; Part 1), which details the general synoptic conditions associated with stream chemistry variations, this study focuses on the long‐term frequency, trends, and global‐scale forcing mechanisms of individual, chemistry‐relevant, synoptic types. Nine individual types are identified as the most important to stream chemistry exports in northeastern watersheds during a 2.5‐year period of high‐frequency stream chemistry observation (2014–2016). Streamflow chemistry was the most influenced by Northwest Flow synoptic weather types in Vermont, cold front passages associated with Weak Westerly Flow types in Rhode Island, and Southwest Flow (SWF) types in Maryland. Each type provides unique atmospheric conditions that result in variations in precipitation, and thus chemical signals. From 1948 to 2017, the Arctic and North Atlantic Oscillations greatly influenced the interannual frequency of the analyzed synoptic types. When the indices were positively phased, SWF types were more frequent, while Northwest Flow and Weak Westerly Flow types were less frequent. Long‐term trends in synoptic type frequency are, inpart, due to documented changes in these teleconnection indices toward more positively phased configurations. As the climate continues to change into the 21st century, such associations may continue to drive large changes in synoptic type frequency, and thus watershed hydrology and biogeochemistry in the Northeast region.
Omega-3 polyunsaturated fatty acids protect against inflammation through production of LOX and CYP450 lipid mediators: relevance for major depression and for human hippocampal neurogenesis
Authors: Borsini Alessandra , Nicolaou Anna , Camacho-Muñoz Dolores , Kendall Alexandra C. , Di Benedetto Maria Grazia , Giacobbe Juliette , Su Kuan-Pin , Pariante Carmine M.
Published: June 2021

Eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) can exert antidepressant, anti-inflammatory and neuroprotective properties, but the exact molecular mechanism underlying their effects is still not fully understood. We conducted both in vitro and clinical investigations to test which EPA or DHA metabolites are involved in these anti-inflammatory, neuroprotective and antidepressant effects. In vitro, we used the human hippocampal progenitor cell line HPC0A07/03C, and pre-treated cells with either EPA or DHA, followed by interleukin 1beta (IL1β), IL6 and interferon-alpha (IFN-α). Both EPA and DHA prevented the reduction in neurogenesis and the increase in apoptosis induced by these cytokines; moreover, these effects were mediated by the lipoxygenase (LOX) and cytochrome P450 (CYP450) EPA/DHA metabolites, 5-hydroxyeicosapentaenoic acid (HEPE), 4-hydroxydocosahexaenoic acid (HDHA), 18-HEPE, 20-HDHA, 17(18)-epoxyeicosatetraenoic acid (EpETE) and 19(20)-epoxydocosapentaenoic acid (EpDPA), detected here for the first time in human hippocampal neurones using mass spectrometry lipidomics of the supernatant. In fact, like EPA/DHA, co-treatment with these metabolites prevented cytokines-induced reduction in neurogenesis and apoptosis. Moreover, co-treatment with 17(18)-EpETE and 19(20)-EpDPA and the soluble epoxide hydroxylase (sEH) inhibitor, TPPU (which prevents their conversion into dihydroxyeicosatetraenoic acid (DiHETE)/ dihydroxydocosapentaenoic acid (DiHDPA) metabolites) further enhanced their neurogenic and anti-apoptotic effects. Interestingly, these findings were replicated in a sample of n = 22 patients with a DSM-IV Major Depressive Disorder, randomly assigned to treatment with either EPA (3.0 g/day) or DHA (1.4 g/day) for 12 weeks, with exactly the same LOX and CYP450 lipid metabolites increased in the plasma of these patients following treatment with their precursor, EPA or DHA, and some evidence that higher levels of these metabolites were correlated with less severe depressive symptoms. Overall, our study provides the first evidence for the relevance of LOX- and CYP450-derived EPA/DHA bioactive lipid metabolites as neuroprotective molecular targets for human hippocampal neurogenesis and depression, and highlights the importance of sEH inhibitors as potential therapeutic strategy for patients suffering from depressive symptoms.
AMRFinderPlus and the Reference Gene Catalog facilitate examination of the genomic links among antimicrobial resistance, stress response, and virulence
Authors: Feldgarden Michael , Brover Vyacheslav , Gonzalez-Escalona Narjol , Frye Jonathan G. , Haendiges Julie , Haft Daniel H. , Hoffmann Maria , Pettengill James B. , Prasad Arjun B. , Tillman Glenn E. , Tyson Gregory H. , Klimke William
Published: June 2021

Antimicrobial resistance (AMR) is a significant public health threat. With the rise of affordable whole genome sequencing, in silico approaches to assessing AMR gene content can be used to detect known resistance mechanisms and potentially identify novel mechanisms. To enable accurate assessment of AMR gene content, as part of a multi-agency collaboration, NCBI developed a comprehensive AMR gene database, the Bacterial Antimicrobial Resistance Reference Gene Database and the AMR gene detection tool AMRFinder. Here, we describe the expansion of the Reference Gene Database, now called the Reference Gene Catalog, to include putative acid, biocide, metal, stress resistance genes, in addition to virulence genes and species-specific point mutations. Genes and point mutations are classified by broad functions, as well as more detailed functions. As we have expanded both the functional repertoire of identified genes and functionality, NCBI released a new version of AMRFinder, known as AMRFinderPlus. This new tool allows users the option to utilize only the core set of AMR elements, or include stress response and virulence genes, too. AMRFinderPlus can detect acquired genes and point mutations in both protein and nucleotide sequence. In addition, the evidence used to identify the gene has been expanded to include whether nucleotide or protein sequence was used, its location in the contig, and presence of an internal stop codon. These database improvements and functional expansions will enable increased precision in identifying AMR genes, linking AMR genotypes and phenotypes, and determining possible relationships between AMR, virulence, and stress response.
Aptamer based diagnosis of crimean-congo hemorrhagic fever from clinical specimens
Authors: Jalali Tahmineh , Salehi-Vaziri Mostafa , Pouriayevali Mohammad Hassan , Gargari Seyed Latif Mousavi
Published: June 2021

Crimean-Congo hemorrhagic fever (CCHF) is an acute viral zoonotic disease. The widespread geographic distribution of the disease and the increase in the incidence of the disease from new regions, placed CCHF in a list of public health emergency contexts. The rapid diagnosis, in rural and remote areas where the majority of cases occur, is essential for patient management. Aptamers are considered as a specific and sensitive tool for being used in rapid diagnostic methods. The Nucleoprotein (NP) of the CCHF virus (CCHFV) was selected as the target for the isolation of aptamers based on its abundance and conservative structure, among other viral proteins. A total of 120 aptamers were obtained through 9 rounds of SELEX (Systematic Evolution of Ligands by Exponential Enrichment) from the ssDNA aptamer library, including the random 40-nucleotide ssDNA region between primer binding sites (GCCTGTTGTGAGCCTCCTAAC(N40)GGGAGACAAGAATAAGCA). The KD of aptamers was calculated using the SPR technique. The Apt33 with the highest affinity to NP was selected to design the aptamer-antibody ELASA test. It successfully detected CCHF NP in the concentration of 90 ng/ml in human serum. Evaluation of aptamer-antibody ELASA with clinical samples showed 100% specificity and sensitivity of the test. This simple, specific, and the sensitive assay can be used as a rapid and early diagnosis tool, as well as the use of this aptamer in point of care test near the patient. Our results suggest that the discovered aptamer can be used in various aptamer-based rapid diagnostic tests for the diagnosis of CCHF virus infection.
Stress in obstructive sleep apnea
Authors: Wong Jasmine L. , Martinez Fernando , Aguila Andrea P. , Pal Amrita , Aysola Ravi S. , Henderson Luke A. , Macey Paul M.
Published: June 2021

People with obstructive sleep apnea (OSA) often have psychological symptoms including depression and anxiety, which are commonly treated with anti-depression or anti-anxiety interventions. Psychological stress is a related symptom with different intervention targets that may also improve mental state, but this symptom is not well characterized in OSA. We therefore aimed to describe stress in relation to other psychological symptoms. We performed a prospective cross-sectional study of 103 people, 44 untreated OSA (mean ± s.d. age: 51.2 ± 13.9 years, female/male 13/31) and 57 healthy control participants (age: 46.3 ± 13.8 years, female/male 34/23). We measured stress (Perceived Stress Scale; PSS), excessive daytime sleepiness (Epworth Sleepiness Scale; ESS), depressive symptoms (Patient Health Questionnaire; PHQ-9), and anxiety symptoms (General Anxiety Disorder; GAD-7). We compared group means with independent samples t-tests and calculated correlations between variables. Mean symptom levels were higher in OSA than control, including PSS (mean ± s.d.: OSA = 15.3 ± 6.9, control = 11.4 ± 5.5; P = 0.002), GAD-7 (OSA = 4.8 ± 5.0, control = 2.1 ± 3.9; P = 0.02), PHQ-9 (OSA = 6.9 ± 6.1, control = 2.6 ± 3.8; P = 0.003) and ESS (OSA = 8.1 ± 5.3, control = 5.0 ± 3.3; P = 0.03). Similar OSA-vs-control differences appeared in males, but females only showed significant differences in PHQ-9 and ESS, not PSS or GAD-7. PSS correlated strongly with GAD-7 and PHQ-9 across groups (R = 0.62–0.89), and moderately with ESS. Perceived stress is high in OSA, and closely related to anxiety and depressive symptoms. The findings support testing stress reduction in OSA.
A modified in vitro clot lysis assay predicts outcomes and safety in acute ischemic stroke patients undergoing intravenous thrombolysis
Authors: Orbán-Kálmándi Rita , Szegedi István , Sarkady Ferenc , Fekete István , Fekete Klára , Vasas Nikolett , Berényi Ervin , Csiba László , Bagoly Zsuzsa
Published: June 2021

The outcome of intravenous thrombolysis using recombinant tissue plasminogen activator (rt-PA) is only favorable in ≈ 40% of acute ischemic stroke (AIS) patients. Moreover, in ≈ 6–8% of cases, intracerebral hemorrhage (ICH) develops. We tested whether a modification of clot lysis assay (CLA), might predict therapy outcomes and safety. In this prospective observational study, blood samples of 231 AIS patients, all receiving intravenous rt-PA, were taken before thrombolysis. Cell-free DNA (cfDNA), CLA and CLA supplemented with cfDNA and histones (mCLA) were determined from the blood samples. Stroke severity was determined by NIHSS on admission. ICH was classified according to ECASSII. Short- and long-term outcomes were defined at 7 and 90 days post-event according to ΔNIHSS and by the modified Rankin Scale, respectively. Stroke severity demonstrated a step-wise positive association with cfDNA levels, while a negative association was found with the time to reach 50% lysis (50%CLT) parameter of CLA and mCLA. ROC analysis showed improved diagnostic performance of the mCLA. Logistic regression analysis proved that 50%CLT is a predictor of short-term therapy failure, while the AUC parameter predicts ICH occurrence. A modified CLA, supplemented with cfDNA and histones, might be a promising tool to predict short-term AIS outcomes and post-lysis ICH.
Cryptochrome expression in avian UV cones: revisiting the role of CRY1 as magnetoreceptor
Authors: Pinzon-Rodriguez Atticus , Muheim Rachel
Published: June 2021

Cryptochromes (CRY) have been proposed as putative magnetoreceptors in vertebrates. Localisation of CRY1 in the UV cones in the retinas of birds suggested that it could be the candidate magnetoreceptor. However, recent findings argue against this possibility. CRY1 is a type II cryptochrome, a subtype of cryptochromes that may not be inherently photosensitive, and it exhibits a clear circadian expression in the retinas of birds. Here, we reassessed the localisation and distribution of CRY1 in the retina of the zebra finch. Zebra finches have a light-dependent magnetic compass based on a radical-pair mechanism, similar to migratory birds. We found that CRY1 colocalised with the UV/V opsin (SWS1) in the outer segments of UV cones, but restricted to the tip of the segments. CRY1 was found in all UV cones across the entire retina, with the highest densities near the fovea. Pre-exposure of birds to different wavelengths of light did not result in any difference in CRY1 detection, suggesting that CRY1 did not undergo any detectable functional changes as result of light activation. Considering that CRY1 is likely not involved in magnetoreception, our findings open the possibility for an involvement in different, yet undetermined functions in the avian UV/V cones.
Prosocial behavior of wearing a mask during an epidemic: an evolutionary explanation
Authors: Kabir K. M. Ariful , Risa Tori , Tanimoto Jun
Published: June 2021

In the midst of the COVID-19 pandemic, with limited or no supplies of vaccines and treatments, people and policymakers seek easy to implement and cost-effective alternatives to combat the spread of infection during the pandemic. The practice of wearing a mask, which requires change in people’s usual behavior, may reduce disease transmission by preventing the virus spread from infectious to susceptible individuals. Wearing a mask may result in a public good game structure, where an individual does not want to wear a mask but desires that others wear it. This study develops and analyzes a new intervention game model that combines the mathematical models of epidemiology with evolutionary game theory. This approach quantifies how people use mask-wearing and related protecting behaviors that directly benefit the wearer and bring some advantage to other people during an epidemic. At each time-step, a suspected susceptible individual decides whether to wear a facemask, or not, due to a social learning process that accounts for the risk of infection and mask cost. Numerical results reveal a diverse and rich social dilemma structure that is hidden behind this mask-wearing dilemma. Our results highlight the sociological dimension of mask-wearing policy.
Preclinical study to improve microbubble-mediated drug delivery in cancer using an ultrasonic probe with an interchangeable acoustic lens
Authors: Lee Seunghyun , Jeon Hoyoon , Shim Shinyong , Im Maesoon , Kim Jinsik , Kim Jung Hoon , Lee Byung Chul
Published: June 2021

Focused ultrasound with microbubbles (FUS-MBs) has shown that it can lead to an efficient drug delivery system (DDS) involving the oscillation and destruction of the MB but is limited in drug delivery due to its narrow pressure field. However, unfocused ultrasound with MBs (UUS-MBs) and an interchangeable acoustic lens can tune and enhance the pressure field for MB destruction to overcome the disadvantages of FUS-MB DDSs. We designed a lens suitable for an ultrasound-phased array probe and studied the optimal treatment conditions for MB destruction in vitro through an optical imaging setup. The DDS effects were evaluated in a rat hepatoma model using doxorubicin (DOX) treatment. A concave lens with a radius of curvature of 2.6 mm and a thickness of 4 mm was selected and fabricated. UUS-MBs with the acoustic lens at 60 Vpp for 32 cycles and a PRF of 1 kHz could induce MB destruction, promoting the DDS even under fluidic conditions. In the animal experiment, the UUS-MBs in the acoustic lens treatment group had a higher concentration of DOX in the tumor than the control group. Our system suggests uses an acoustic lens to increase DDS effectiveness by providing sufficient ultrasound irradiation to the MBs.
Observation of frequency-uncorrelated photon pairs generated by counter-propagating spontaneous parametric down-conversion
Authors: Liu Yi-Chen , Guo Dong-Jie , Ren Kun-Qian , Yang Ran , Shang Minghao , Zhou Wei , Li Xinhui , Sun Chang-Wei , Xu Ping , Xie Zhenda , Gong Yan-Xiao , Zhu Shi-Ning
Published: June 2021

We report the generation of frequency-uncorrelated photon pairs from counter-propagating spontaneous parametric down-conversion in a periodically-poled KTP waveguide. The joint spectral intensity of photon pairs is characterized by measuring the corresponding stimulated process, namely, the difference frequency generation process. The experimental result shows a clear uncorrelated joint spectrum, where the backward-propagating photon has a narrow bandwidth of 7.46 GHz and the forward-propagating one has a bandwidth of 0.23 THz like the pump light. The heralded single-photon purity estimated through Schmidt decomposition is as high as 0.996, showing a perspective for ultra-purity and narrow-band single-photon generation. Such unique feature results from the backward-wave quasi-phase-matching condition and does not has a strict limitation on the material and working wavelength, thus fascinating its application in photonic quantum technologies.
Genetic variants in bone morphogenetic proteins signaling pathway might be involved in palatal rugae phenotype in humans
Authors: Silva-Sousa Alice Corrêa , Marañón-Vásquez Guido Artemio , Stuani Maria Bernadete Sasso , Proff Peter , Andrades Kesly Mary Ribeiro , Baratto-Filho Flares , Matsumoto Mírian Aiko Nakane , Paddenberg Eva , Küchler Erika Calvano , Kirschneck Christian
Published: June 2021

This study investigated, if genetic variants in BMP2, BMP4 and SMAD6 are associated with variations in the palatal rugae pattern in humans. Dental casts and genomic DNA from 75 patients were evaluated. Each patient was classified as follows: total amount of rugae; bilateral symmetry in the amount, length and shape of the palatal rugae; presence of secondary or fragmentary palatal rugae; presence of unifications; predominant shape; and predominant direction of the palatal rugae. The genetic variants in BMP2 (rs1005464 and rs235768), BMP4 (rs17563) and SMAD6 (rs2119261 and rs3934908) were genotyped. Genotype distribution was compared between palatal rugae patterns using the chi-square test (alpha = 0.05). The allele A was associated with the presence of secondary or fragmentary rugae for rs1005464 (OR = 2.5, 95%CI 1.1–6.3; p = 0.014). Secondary or fragmentary rugae were associated with the G allele in rs17563 (OR = 2.1, 95%CI 1.1–3.9; p = 0.017). rs17563 was also associated with rugae unification (p = 0.017 in the additive model). The predominant shape (wavy) was associated with rs2119261 (p = 0.023 in the additive model). The left–right symmetry of the length of primary rugae was associated with rs3934908 in the recessive model (OR = 3.6, 95%CI 1.2–11.7; p = 0.025). In conclusion, genetic variants in the BMP pathway impacted on palatal rugae pattern.