bluuebunny/crossref_metadata_2025 huggingface dataset:
Acquisition pattern and the role of vocabulary and language experience in the acquisition of inflectional grammar by Mandarin-English speaking preschoolers
Authors: Xu Rattanasone Nan , Kim Jae-Hyun
Published: February 2024

Australian Mandarin-English bilingual preschoolers must acquire linguistic structures that occur only in the community language (e.g., English inflectional grammar). This study investigated how they acquire such structures and any relationship between linguistic knowledge and language experience on their performance. Twenty 4–6-year-olds showed known monolingual acquisition patterns with good performance for producing the progressive, developing ability for plurals, but only emerging ability for past and present tense. Better performance was related to a larger English vocabulary, more mixed language input and use, but less Mandarin input and use. On average, these children received less than 50% input in English and were performing behind monolinguals.
Syncopation as structure bootstrapping: the role of asymmetry in rhythm and language
Authors: Fiorin Gaetano , Delfitto Denis
Published: February 2024

Syncopation – the occurrence of a musical event on a metrically weak position preceding a rest on a metrically strong position – represents an important challenge in the study of the mapping between rhythm and meter. In this contribution, we present the hypothesis that syncopation is an effective strategy to elicit the bootstrapping of a multi-layered, hierarchically organized metric structure from a linear rhythmic surface. The hypothesis is inspired by a parallel with the problem of linearization in natural language syntax, which is the problem of how hierarchically organized phrase-structure markers are mapped onto linear sequences of words. The hypothesis has important consequences for the role of meter in music perception and cognition and, more particularly, for its role in the relationship between rhythm and bodily entrainment.
Reevaluating hikikomori and challenging loneliness assumptions in Japan: A cross-sectional analysis of a nationwide internet sample
Authors: Yong Roseline
Published: February 2024

Introduction

Loneliness in Japan, accentuated by demographic challenges and the hikikomori phenomenon (extreme social withdrawal), has raised concerns. This study critically examines loneliness dynamics, questioning assumptions embedded in hikikomori classifications. The term “hikikomori,” originally signifying prolonged home stay, requires nuanced exploration, especially regarding outgoing behaviors’ relationship with loneliness.

Objectives

Investigating the intricate connection between outgoing behaviors and loneliness, this study questions the effectiveness of existing hikikomori classifications. Aiming to understand if these classifications accurately represent the loneliness spectrum across age groups, the research emphasizes the significance of comprehending loneliness dynamics amid societal challenges. The study explores an array of factors influencing loneliness, including demographics, mental health, and outgoing behaviors, advocating for a reassessment of assumptions linked to hikikomori classifications.

Methods

This secondary analysis employed data from a nationwide Internet addiction survey conducted in July 2012. A sample of 623 participants, representative of Japanese internet users aged 16 and above, was included for analysis. Loneliness was assessed using the UCLA Loneliness Scale, and exposure variables included demographic, mental health, outgoing behaviors, and lifestyle factors. Statistical analyses encompassed descriptive statistics, one-way ANOVA, chi-square tests, and logistic regression.

Results

Significant differences were observed in loneliness scores based on sex, age, marital status, employment, and outgoing behaviors. Mental health factors, including dissatisfaction with life and romance, life stress, and psychological distress, emerged as strong contributors to loneliness. The study challenges existing hikikomori classifications, suggesting they may not fully encapsulate the loneliness experiences of individuals engaged in routine school or work activities.

Conclusion

Findings underscore the need for a reevaluation of hikikomori, emphasizing loneliness as a complex and multifaceted issue in Japan. The study advocates for nuanced strategies to address loneliness, considering diverse demographic vulnerabilities. Limitations include the pre-pandemic sample and potential unmeasured confounding factors.
Psychosocial stress moderates the relationship between cerebrospinal fluid lactate dehydrogenase and the duration of untreated psychosis in first-episode psychosis
Authors: Giné-Servén Eloi , Boix-Quintana Ester , Daví-Loscos Eva , Cepedello Sandra , Moreno-Sancho Lara , Niubó Marta , Hernández-Antón Rebeca , Cuesta Manuel J. , Labad Javier
Published: February 2024

Introduction

Previous research has shown that lower lactate dehydrogenase (LDH) concentrations in cerebrospinal fluid (CSF) are associated with longer prodromal symptoms in first-episode psychosis (FEP). We aimed to study whether there is a relationship between the duration of untreated psychosis (DUP) and LDH and other CSF biomarkers in FEP and whether stressful life events moderate this association.

Methods

Ninety-five inpatients with FEP and with less than 6 weeks of antipsychotic treatment were included in the study. All participants were informed about the nature of the study, which was approved by the local ethics committee, and signed an informed consent form. A lumbar puncture was performed at index admission (baseline) to measure CSF parameters (glucose, total protein, LDH). The DUP was assessed with the Quick Psychosis Onset and Prodromal Symptoms Inventory (Q-POPSI). Stressful life events (SLEs) in the previous 6 months were assessed with the List of Threatening Experiences. We dichotomized the SLE variable into having experienced at least one SLE or no experience of SLEs. Statistical analyses were performed with SPSS v. 25.0. Total protein and LDH concentrations were natural log transformed (ln) to reduce skewness. Multiple linear regression analyses were conducted to explore the association between the DUP and CSF parameters (considered the dependent variable). Age, sex, DUP and SLEs were considered independent variables. We tested the DUP by SLE interaction. Significant interactions were included in the final model. The threshold for significance was set at p<0.05.

Results

Fifty-four FEP patients (56.8%) reported an SLE in the previous 6 months. There were no significant differences in the DUP between patients with or without SLEs. There were no significant differences in CSF biomarkers between the SLE groups. In the multiple linear regression analyses, we found a significant DUP by SLE interaction effect on CSF LDH concentrations (standardized beta= -0.320, t= -2.084, p= 0.040). In patients with SLEs, a shorter DUP was associated with higher CSF LDH concentrations and vice versa. No significant associations were found between the DUP or SLEs and other CSF biomarkers (glucose, total proteins).

Conclusions

Our study suggests that psychosocial stress moderates the relationship between the onset of psychosis and CSF biomarkers related to bioenergetic systems.
MtDNA deletions and aging
Authors: Sprason Charlotte , Tucker Trudy , Clancy David
Published: February 2024

Aging is the major risk factor in most of the leading causes of mortality worldwide, yet its fundamental causes mostly remain unclear. One of the clear hallmarks of aging is mitochondrial dysfunction. Mitochondria are best known for their roles in cellular energy generation, but they are also critical biosynthetic and signaling organelles. They also undergo multiple changes with organismal age, including increased genetic errors in their independent, circular genome. A key group of studies looking at mice with increased mtDNA mutations showed that premature aging phenotypes correlated with increased deletions but not point mutations. This generated an interest in mitochondrial deletions as a potential fundamental cause of aging. However, subsequent studies in different models have yielded diverse results. This review summarizes the research on mitochondrial deletions in various organisms to understand their possible roles in causing aging while identifying the key complications in quantifying deletions across all models.
Development and initial evaluation of the usefulness of a question prompt list to promote patients' level of information about work-related medical rehabilitation: a pilot study
Authors: Lukasczik Matthias , Wolf Hans Dieter , Vogel Heiner
Published: February 2024

Introduction

The purpose of this study was to develop a question prompt list (QPL) to support patients undergoing work-related medical rehabilitation in obtaining relevant information and to explore how patients and physicians rate the QPL regarding its usefulness, practicability, and perceived (additional) effort.

Methods

An initial item pool was assessed by rehabilitation patients (N = 3) in cognitive interviews, rated by physicians and other health professionals (N = 11), and then further modified. The final QPL version (16 items) was used by patients (N = 36) in medical admission interviews in an inpatient medical rehabilitation facility and then evaluated. Physicians evaluated the QPL after each interview with a study participant (N = 6; k = 39 interviews).

Results

The QPL was used by 50% of patients who rated its usefulness and comprehensibility positively. Neither the need for information nor satisfaction with the information received was correlated with QPL use. The physicians' assessment showed a positive evaluation regarding the provision of information and structuring of the conversation, but also a higher perceived time expenditure.

Discussion

While initial testing of the QPL in work-related medical rehabilitation as a tool to support patient-provider communication generally showed a favorable evaluation by patients using it and physicians, future research should address its validity and effectiveness.
Raising patient voices in medical education: an assessment of patient perceived effect of social determinants of health conversations and the patient-physician relationship on quality of obstetric care, to inform the development of patient driven medical education curricula
Authors: Brito S. , Rau A. , Escobar C. , Garza P. , Sriprasert I. , Mitchell Chadwick N.
Published: February 2024

Background

Conventional medical education lacks the lived experiences of patients which may authentically convey the social determinants of health (SDOH) and resulting health disparities. Videos of first-person patient narratives may prove a valuable education tool in this regard. The objective of this study is to investigate how patient demographics, satisfaction with care, and patient-physician relationships influence obstetric patient interest and willingness to contribute to a SDOH video curriculum by sharing their lived experiences through first-person narratives.

Methods

Study design included an anonymous, cross-sectional survey and an optional semi-structured telephone interview. Participants were 18 years old with a live-birth delivery <8 weeks prior to recruitment and received care during their pregnancy at Los Angeles General Medical Center (LAGMC). Variables surveyed included demographics, satisfaction with care, aspects of the patient-physician relationship, perceived utility, and personal interest in contributing to an educational SDOH video. A bivariate analysis was conducted to compare participants’ characteristics and responses on interest in contributing and perceived helpfulness of first-person patient SDOH videos.

Results

72.43% of participants (N = 70) believed a patient's first-person video on SDOH would be “Helpful” in preparing physicians to provide competent medical care; however, 71.43% responded “No” to “Interest” in sharing with physicians their experiences with SDOH. English preference and being U.S. born were factors significantly associated with viewing first-person SDOH video as “Helpful” (P > 0.001). Major themes from telephone interviews reflected enthusiasm for first-person patient narratives and perceived benefits of using patient experiences to educate physicians on SDOH. However, participants cited barriers to disclosing SDOH including brief and strictly clinical interactions with physicians, lack of continuity of care, and fear of being judged by physicians.

Conclusion

While most participants recognized the utility of addressing social needs in medical education and reported satisfaction with their obstetricians and care, these factors did not uniformly translate into willingness to contribute first-person patient narratives. To improve the representation of patients from racial, ethnic, gender, linguistic, and sexual minorities into medical curricula, further research and strategies are needed to overcome the barriers discouraging patient disclosure of social needs to physicians.
The portrayal of gender in Marvel and Star Wars media targeted towards children
Authors: Clarke Lucy Louise , Hine Benjamin
Published: February 2024

An abundance of previous research has investigated how gender has been portrayed within feature length films produced by Walt Disney Animation Studios, particularly those within the Disney princess franchise. However, the Disney corporation acquired the Marvel and Star Wars franchises in 2009 and 2012, respectively, which was likely a strategy for the corporation to obtain characters that would capture the imagination of boys and men. The current qualitative study explored how gender is portrayed by leading protagonists in these texts, utilising thematic analysis, which was necessary considering little is currently known in this domain. The researchers analysed series one of Avengers Assemble and series one of Star Wars Rebels. Interpretation of the data led to the development of several themes and subthemes based on the gendered portrayals within each series. Overall, the findings suggest that there was more overt gender stereotyping in Avengers Assemble when compared with Star Wars Rebels, meaning that the former could be particularly problematic for children who may replicate its messages. The current study has facilitated a greater understanding of the gendered messages that may be consumed by children who engage with Marvel and Star Wars media. Future research is needed to assess the relationship between such messages and children’s behaviour.
Observations key to understanding solar cycles: a review
Authors: Martin Sara F.
Published: February 2024

A paradigm shift is taking place in the conception of solar cycles. In the previous conception, the changing numbers of sunspots over intervals of 9–14 years have been regarded as the fundamental solar cycle although two average 11-year cycles were necessary to account for the complete magnetic cycle. In the revised picture, sunspots are a phase in the middle of two 22-year overlapping solar cycles that operate continuously with clock-like precision. More than 20 researchers have contributed to the initial research articles from 2014 through 2021 which are dramatically altering the perception of solar cycles. The two 22-year cycles overlap in time by 11 years. This overlap is coincidentally the same average duration as the sunspot phase in each 22-year cycle. This coincidence and the relative lack of knowledge of the large numbers of small active regions without sunspots is what led to the previous paradigm in which the 11-year sunspot phases were misinterpreted as a single fundamental solar cycle. The combination of the two 22-year solar cycles, with their large numbers of short-lived active regions and ephemeral active regions are now understood to be the fundamental cycle with the proposed name “The Hale Solar Cycle.” The two 22-year solar cycles each occupy separate but adjacent bands in latitude. The orientations of the majority of bipolar magnetic regions in the two adjacent bands differ from each other by ∼180°. Both bands continuously drift from higher to lower latitudes as has been known for sunspot cycles. However, the polarity reversal occurs at the start of each 22-year cycle and at higher latitudes than it does for the sunspot cycles. This paradigm shift in the concept of solar cycles has resulted in major reconsiderations of additional topics on solar cycles in this review. These are 1) the large role of ephemeral active regions in the origin of solar cycles, 2) the depth of the origin of active regions and sunspots, 3) the mechanisms of how areas of unipolar magnetic network migrate to the solar poles every 11 years, and 4) the nature of the polarity reversal in alternate 22-year cycles rather than 11-year cycles.
Augmentation of intramedullary nail in unstable intertrochanteric fractures with plate or cable
Authors: Zhao Yanrui , Wang Hanzhou , Liu Yang , Shan Lei , Zhou Junlin
Published: February 2024

Backgrounds

This study aims to evaluate the clinical outcome of intramedullary nail supplemented by buttress plate or cable in the treatment of intertrochanteric fracture with broken lateral wall.

Methods

From May 2015 to January 2022, patients with unstable intertrochanteric femoral fractures underwent intramedullary fixations strengthened with buttress plates or cables, which depended on the lateral femur wall fragment type. The clinical and radiographic results were compared between the two groups. The hip function was evaluated according to the Harris Hip Scoring (HHS) system.

Results

Forty-one patients who sustained intertrochanteric fracture + broken lateral wall were enrolled. Of these, thirty-four received a minimum of twelve months of follow-ups. No statistically significant differences in baseline and operative data were proved between these groups (p > 0.05). Three patients were observed fat liquefaction after surgery (plate group: 2 cases, cable group: 1 case). All patients could sustain partial/full weight-bearing and no case underwent subsequent operation. The HHS of the last follow-up presented 83.6 ± 4.9 points in the plate group and 83.8 ± 3.7 points in the cable group.

Conclusions

Intertrochanteric femoral fracture with broken lateral wall is an unstable injury type, the operative treatments of which have been challenging and controversial over the years. Augmentation of intramedullary nailing system using plate/cable contributes to reconstructing the lateral femur wall.
Controlling Coxiella burnetii in naturally infected sheep, goats and cows, and public health implications: a scoping review
Authors: Toledo-Perona Raquel , Contreras Antonio , Gomis Jesús , Quereda Juan José , García-Galán Ana , Sánchez Antonio , Gómez-Martín Ángel
Published: February 2024

Q fever is a worldwide zoonotic disease which domestic ruminants are the main source of infection for humans. This scoping review summarizes the control measures currently available to reduce Coxiella burnetii (Cb) infection in naturally infected sheep, goat and cattle herds. A total of 28 articles were included in the review. A lack of methodological standardization was noted in the articles analyzed. The results indicated that long-term vaccination in cows reduces bacterial excretion in milk and environmental contamination. In small ruminants, the results of vaccination in terms of efficacy are variable. In goats, there is a reduction in bacterial excretion, unlike in sheep, where a long-term vaccination program is necessary to reduce bacterial excretion. Moreover, the high persistence of viable Cb in the environment means that control measures for sheep are needed for several years. The use of antibiotics as a control measure in cows and sheep was not found to reduce excretion. However, the combination of vaccination with antibiotic therapy appears to have positive effects in small ruminants in terms of controlling outbreaks of Q fever. Hygiene and biosecurity measures are the basic means for controlling Cb infection on ruminant farms and ensuring public health.
Online Deterministic 3D Trajectory Generation for Electric Vertical Take-Off and Landing Aircraft
Authors: Mbikayi Zoe , Steinert Agnes , Heimsch Dominik , Speckmaier Moritz , Rudolph Philippe , Liu Hugh , Holzapfel Florian
Published: February 2024

The use of non-piloted eVTOL aircraft in non-segregated airspace requires reliable and deterministic automatic flight guidance systems for the aircraft to remain predictable to all the users of the airspace and maintain a high level of safety. In this paper we present a 3D trajectory generation module based on clothoid transition segments in the horizontal plane and high order polynomial transition segments in the vertical plane. The expressions of the coefficients of the polynomial are derived offline are used to generate the trajectory online, making the system capable of running in real time without requiring enormous computational power. For the horizontal plane, we focus on the flyby transition, and therefore present a thorough analysis of the flyby geometry and the limitations linked to this geometry and the construct of three-segment trajectory generation around a fixed turn rate. We present feasible solutions for these limitations, and show simulation results for the combined horizontal and vertical plane concepts, allowing the system to generate complex 3D trajectories.
Effects of Dietary Alfalfa Meal Supplementation on the Growth Performance, Nutrient Apparent Digestibility, Serum Parameters, and Intestinal Microbiota of Raccoon Dogs (Nyctereutes procyonoides)
Authors: Chen Xiaoli , Li Xiao , Chen Danyang , Zhao Weigang , Zhang Xiuli , Yuan Weitao , Si Huazhe , Deng Xuming , Du Rui , Xu Chao
Published: February 2024

The raccoon dog (Nyctereutes procyonoides) is a typical omnivore possessing wide dietary adaptability and tolerance to rough feeding, which may be attributed to its intestinal microbiota. The study aimed to investigate the effect of dietary alfalfa meal levels on the growth performance, nutrient apparent digestibility, serum parameters, and intestinal microbiota of raccoon dogs. Sixty raccoon dogs were randomly divided into four dietary treatments containing 0% (AM0), 5% (AM5), 10% (AM10), and 15% (AM15) alfalfa meal for a 60-day experiment. The results showed that compared to raccoon dogs fed the AM0 diet, those fed the AM5 and AM10 diets had no significant difference in growth performance, while those fed the AM15 diet experienced a significant decrease. Raccoon dogs fed the AM5 diet had no significant effect on the nutrient apparent digestibility. Dietary supplementation with alfalfa meal significantly decreased serum urea levels and increased the antioxidant capacity of raccoon dogs. The intestinal microbiome analysis showed that the richness and diversity of colonic microbiota significantly increased in the AM15 group. With the increase in dietary alfalfa meal levels, the relative abundance of fiber-degrading bacteria in the colon of raccoon dogs, such as Treponema, Phascolarctobacterium, and Christensenellaceae R-7 group, increased. However, the relative abundance of pathogenic bacteria, including Anaerobiospirillum, decreased. In conclusion, the inclusion of 5% alfalfa meal in the raccoon dogs’ diet had no effect on growth performance, but it exhibited the potential to improve serum antioxidant capacity and intestinal microbiota. This indicates that raccoon dogs have a certain tolerance to the addition of alfalfa meal in their diet.
Adaptive Integrated Thermal Management System for a Stable Driving Environment in Battery Electric Vehicles
Authors: Bae Jaehyun , Hyun Daeil , Han Jaeyoung
Published: February 2024

With an increase in global warming, battery electric vehicles (BEVs), which are environmentally friendly, have been rapidly commercialized to replace conventional vehicles with internal combustion engines. Unlike traditional internal combustion engine vehicles, the powertrain system of BEVs operates with high efficiency, resulting in lower heat generation. This poses a challenge for cabin heating under low-temperature conditions. Conversely, under high-temperature conditions, the operating temperature of a high-voltage battery (HVB) is lower than the ambient air temperature, which makes cooling through ambient air challenging. To overcome these challenges, in this study, we proposed an integrated thermal management system (ITMS) based on a heat pump system capable of stable thermal management under diverse climatic conditions. Furthermore, to assess the ability of the proposed ITMS to perform thermal management under various climatic conditions, we integrated a detailed powertrain system model incorporating BEV specifications and the proposed ITMS model based on the heat pump system. The ITMS model was evaluated under high-load-driving conditions, specifically the HWFET scenario, demonstrating its capability to perform stable thermal management not only under high-temperature conditions, such as at 36 °C, but also under low-temperature conditions, such as at −10 °C, through the designated thermal management modes.
Proteolytic Vesicles Derived from Salmonella enterica Serovar Typhimurium-Infected Macrophages: Enhancing MMP-9-Mediated Invasion and EV Accumulation
Authors: Nudelman Alon , Shenoy Anjana , Allouche-Arnon Hyla , Fisler Michal , Rosenhek-Goldian Irit , Dayan Lior , Abou Karam Paula , Porat Ziv , Solomonov Inna , Regev-Rudzki Neta , Bar-Shir Amnon , Sagi Irit
Published: February 2024

Proteolysis of the extracellular matrix (ECM) by matrix metalloproteinases (MMPs) plays a crucial role in the immune response to bacterial infections. Here we report the secretion of MMPs associated with proteolytic extracellular vesicles (EVs) released by macrophages in response to Salmonella enterica serovar Typhimurium infection. Specifically, we used global proteomics, in vitro, and in vivo approaches to investigate the composition and function of these proteolytic EVs. Using a model of S. Typhimurium infection in murine macrophages, we isolated and characterized a population of small EVs. Bulk proteomics analysis revealed significant changes in protein cargo of naïve and S. Typhimurium-infected macrophage-derived EVs, including the upregulation of MMP-9. The increased levels of MMP-9 observed in immune cells exposed to S. Typhimurium were found to be regulated by the toll-like receptor 4 (TLR-4)-mediated response to bacterial lipopolysaccharide. Macrophage-derived EV-associated MMP-9 enhanced the macrophage invasion through Matrigel as selective inhibition of MMP-9 reduced macrophage invasion. Systemic administration of fluorescently labeled EVs into immunocompromised mice demonstrated that EV-associated MMP activity facilitated increased accumulation of EVs in spleen and liver tissues. This study suggests that macrophages secrete proteolytic EVs to enhance invasion and ECM remodeling during bacterial infections, shedding light on an essential aspect of the immune response.
Introducing Corrections to the Reflectance of Graphene by Light Emission
Authors: Sasaki Ken-ichi , Hitachi Kenichi , Kamada Masahiro , Yokosawa Takamoto , Ochi Taisuke , Matsui Tomohiro
Published: February 2024

Monolayer graphene absorbs 2.3 percent of the incident visible light. This “small” absorption has been used to emphasize the visual transparency of graphene, but it in fact means that multilayer graphene absorbs a sizable fraction of incident light, which causes non-negligible fluorescence. In this paper, we formulate the light emission properties of multilayer graphene composed of tens to hundreds of layers using a transfer matrix method and confirm the method’s validity experimentally. We quantitatively explain the measured contrasts of multilayer graphene on SiO2/Si substrates and find sizable corrections, which cannot be classified as incoherent light emissions, to the reflectance of visible light. The new component originates from coherent emission caused by absorption at each graphene layer. Multilayer graphene thus functions as a partial coherent light source of various wavelengths, and it may have surface-emitting laser applications.
ATOH1, TFAP2B, and CEACAM6 as Immunohistochemical Markers to Distinguish Merkel Cell Carcinoma and Small Cell Lung Cancer
Authors: Vilasi Serena M. , Nguyen Jannett , Wang Catherine J. , Miao Lingling , Daily Kenneth , Eid Mary , Song Joon Seon , Jiang Hong , Ylaya Kris , Busam Klaus J. , Gaiser Maria R. , Hewitt Stephen M. , Brownell Isaac
Published: February 2024

Merkel cell carcinoma (MCC) and small cell lung cancer (SCLC) can be histologically similar. Immunohistochemistry (IHC) for cytokeratin 20 (CK20) and thyroid transcription factor 1 (TTF-1) are commonly used to differentiate MCC from SCLC; however, these markers have limited sensitivity and specificity. To identify new diagnostic markers, we performed differential gene expression analysis on transcriptome data from MCC and SCLC tumors. Candidate markers included atonal BHLH transcription factor 1 (ATOH1) and transcription factor AP-2β (TFAP2B) for MCC, as well as carcinoembryonic antigen cell adhesion molecule 6 (CEACAM6) for SCLC. Immunostaining for CK20, TTF-1, and new candidate markers was performed on 43 MCC and 59 SCLC samples. All three MCC markers were sensitive and specific, with CK20 and ATOH1 staining 43/43 (100%) MCC and 0/59 (0%) SCLC cases and TFAP2B staining 40/43 (93%) MCC and 0/59 (0%) SCLC cases. TTF-1 stained 47/59 (80%) SCLC and 1/43 (2%) MCC cases. CEACAM6 stained 49/59 (83%) SCLC and 0/43 (0%) MCC cases. Combining CEACAM6 and TTF-1 increased SCLC detection sensitivity to 93% and specificity to 98%. These data suggest that ATOH1, TFAP2B, and CEACAM6 should be explored as markers to differentiate MCC and SCLC.
Dielectric Characterization of Ex-Vivo Breast Tissues: Differentiation of Tumor Types through Permittivity Measurements
Authors: Fernández-Aranzamendi Elizabeth G. , Castillo-Araníbar Patricia R. , San Román Castillo Ebert G. , Oller Belén S. , Ventura-Zaa Luz , Eguiluz-Rodriguez Gelber , González-Posadas Vicente , Segovia-Vargas Daniel
Published: February 2024

Early analysis and diagnosis of breast tumors is essential for either quickly launching a treatment or for seeing the evolution of patients who, for instance, have already undergone chemotherapy treatment. Once tissues are excised, histological analysis is the most frequent tool used to characterize benign or malignant tumors. Dielectric microwave spectroscopy makes use of an open-ended coaxial probe in the 1–8 GHz frequency range to quickly identify the type of tumor (ductal carcinoma, lobular carcinoma, mucinous carcinoma and fibroadenoma). The experiment was undertaken with data from 70 patients who had already undergone chemotherapy treatment, which helped to electrically map the histological tissues with their electric permittivity. Thus, the variations in the permittivity of different types of tumors reveal distinctive patterns: benign tumors have permittivity values lower than 35, while malignant ones range between 40 and 60. For example, at a frequency of 2 GHz, the measured permittivity was 45.6 for ductal carcinoma, 33.1 for lobular carcinoma, 59.5 for mucinous carcinoma, and 27.6 for benign tumors. This differentiation remains consistent in a frequency range of 1 to 4.5 GHz. These results highlight the effectiveness of these measurements in the classification of breast tumors, providing a valuable tool for quick and accurate diagnosis and effective treatment.
Effects of Group Exercise Intervention on Quality of Life and Physical Parameters in Patients with Childhood Cancer: A Systematic Review
Authors: Doi Yurina , Ogawa Masato , Ishihara Kodai , Inoue Junichiro , Izawa Kazuhiro P.
Published: February 2024

Background: Although the survival rates of childhood cancer are increasing, children diagnosed as having cancer experience psychological and physical problems and a declining quality of life (QOL). Methods: A systematic review of PubMed databases was conducted up to September 2023 to identify studies reporting the effects of group exercise intervention in children with cancer. The inclusion criteria were pre-specified, including children aged ≤19 years old who received group exercise intervention and interventional studies written in English. Studies involving non-exercise intervention or non-group intervention were excluded. Results: Five studies were included in the present review. In three studies, QOL and physical parameters were improved after group exercise intervention, and in two studies, only physical parameters were improved. Improvements in QOL were achieved through psychosocial variables, improved scores of subscales of pain and hurt, nausea, and procedure-related anxiety, and reduced cancer-related fatigue. All studies had high numbers of participants who completed the intervention. However, all studies showed a high risk of bias regarding the selection of the reported results, and most studies showed a high risk of bias regarding deviations from the intended intervention and outcome measurement. Conclusion: The reviewed studies showed that group exercise intervention for children with cancer could improve their QOL and/or physical parameters.
Evaluating the Use of a Thermal Sensor to Detect Small Ground-Nesting Birds in Semi-Arid Environments during Winter
Authors: Avila-Sanchez J. Silverio , Perotto-Baldivieso Humberto L. , Massey Lori D. , Ortega-S. J. Alfonso , Brennan Leonard A. , Hernández Fidel
Published: February 2024

Aerial wildlife surveys with fixed-wing airplanes and helicopters are used more often than on-the-ground field surveys to cover areas that are both extensive and often inaccessible. Drones with high-resolution thermal sensors are being widely accepted as research tools to aid in monitoring wildlife species and their habitats. Therefore, our goal was to assess the feasibility of detecting northern bobwhite quail (Colinus virginianus, hereafter ‘bobwhite’) using drones with a high-resolution thermal sensor. Our objectives were (1) to identify the altitudes at which bobwhites can be detected and (2) compare the two most used color palettes to detect species (black-hot and isotherm). We achieved this goal by performing drone flights at different altitudes over caged tame bobwhites and capturing still images and video recordings at altitudes from 18 to 42 m. We did not observe or detect any obvious signs of distress, movement, or fluttering of bobwhites inside cages caused by the noise or presence of the drone during data acquisition. We observed the highest counts of individual bobwhites with the black-hot thermal palette at 18 m (92%; x¯ = 47 bobwhites; SE = 0.41) and at 24 m (81%; x¯ = 41 bobwhites; SE = 0.89). The isotherm thermal palette had lower count proportions. The use of video to count quail was not feasible due to the low resolution of the video and the species size. Flying drones with high-resolution thermal sensors provided reliable imagery to detect roosting bobwhite individuals in South Texas during the winter.
The Circumstance-Driven Bivariate Integer-Valued Autoregressive Model
Authors: Wang Huiqiao , Weiß Christian H.
Published: February 2024

The novel circumstance-driven bivariate integer-valued autoregressive (CuBINAR) model for non-stationary count time series is proposed. The non-stationarity of the bivariate count process is defined by a joint categorical sequence, which expresses the current state of the process. Additional cross-dependence can be generated via cross-dependent innovations. The model can also be equipped with a marginal bivariate Poisson distribution to make it suitable for low-count time series. Important stochastic properties of the new model are derived. The Yule–Walker and conditional maximum likelihood method are adopted to estimate the unknown parameters. The consistency of these estimators is established, and their finite-sample performance is investigated by a simulation study. The scope and application of the model are illustrated by a real-world data example on sales counts, where a soap product in different stores with a common circumstance factor is investigated.
Hemodynamic Insights into Abdominal Aortic Aneurysms: Bridging the Knowledge Gap for Improved Patient Care
Authors: Saha Suvash C. , Francis Isabella , Saha Goutam , Huang Xinlei , Molla Md. Mamun
Published: February 2024

Background: Abdominal aortic aneurysms (AAAs) present a formidable public health concern due to their propensity for localized, anomalous expansion of the abdominal aorta. These insidious dilations, often in their early stages, mask the life-threatening potential for rupture, which carries a grave prognosis. Understanding the hemodynamic intricacies governing AAAs is paramount for predicting aneurysmal growth and the imminent risk of rupture. Objective: Our extensive investigation delves into this complex hemodynamic environment intrinsic to AAAs, utilizing comprehensive numerical analyses of the physiological pulsatile blood flow and realistic boundary conditions to explore the multifaceted dynamics influencing aneurysm rupture risk. Our study introduces novel elements by integrating these parameters into the overall context of aneurysm pathophysiology, thus advancing our understanding of the intricate mechanics governing their evolution and rupture. Methods: Conservation of mass and momentum equations are used to model the blood flow in an AAAs, and these equations are solved using a finite volume-based ANSYS Fluent solver. Resistance pressure outlets following a three-element Windkessel model were imposed at each outlet to accurately model the blood flow and the AAAs’ shear stress. Results: Our results uncover elevated blood flow velocities within an aneurysm, suggesting an augmented risk of future rupture due to increased stress in the aneurysm wall. During the systole phase, high wall shear stress (WSS) was observed, typically associated with a lower risk of rupture, while a low oscillatory shear index (OSI) was noted, correlating with a decreased risk of aneurysm expansion. Conversely, during the diastole phase, low WSS and a high OSI were identified, potentially weakening the aneurysm wall, thereby promoting expansion and rupture. Conclusion: Our study underscores the indispensable role of computational fluid dynamic (CFD) techniques in the diagnostic, therapeutic, and monitoring realms of AAAs. This body of research significantly advances our understanding of aneurysm pathophysiology, thus offering pivotal insights into the intricate mechanics underpinning their progression and rupture, informing clinical interventions and enhancing patient care.
Schisandra chinensis Bee Pollen Ameliorates Colitis in Mice by Modulating Gut Microbiota and Regulating Treg/Th17 Balance
Authors: Cheng Ni , Wang Xiaochao , Zhou Yaoyao , Zhao Xuanxuan , Chen Minghao , Zhao Haoan , Cao Wei
Published: February 2024

Colitis is a chronic disease associated with alterations in the composition of gut microbiota. Schisandra chinensis bee pollen extract (SCPE) has been proved to be rich in phenolic compounds and effective in modulating gut microbiota, but its effect on colitis and the underlying mechanism remains unclear. This study investigates the relationship between colitis amelioration and the gut microbiota regulation of SCPE via fecal microbial transplantation (FMT). The results showed that administration of 20.4 g/kg BW of SCPE could primely ameliorate colitis induced by dextran sulfate sodium (DSS) in mice, showing as more integration of colon tissue structure and the colonic epithelial barrier, as well as lower oxidative stress and inflammation levels compared with colitis mice. Moreover, SCPE supplement restored the balance of T regulatory (Treg) cells and T helper 17 (Th17) cells. Gut microbiota analysis showed SCPE treatment could reshape the gut microbiota balance and improve the abundance of gut microbiota, especially the beneficial bacteria (Akkermansia and Lactobacillus) related to the production of short-chain fatty acids and the regulation of immunity. Most importantly, the protection of 20.4 g/kg BW of SCPE on colitis can be perfectly transmitted by fecal microbiota. Therefore, the gut microbiota–SCFAS–Treg/Th17 axis can be the main mechanism for SCPE to ameliorate colitis. This study suggests that SCPE can be a new promising functional food for prevention and treatment of colitis by reshaping gut microbiota and regulating gut immunity.
The Effect of Opuntia ficus Mucilage Pectin and Citrus aurantium Extract Added to a Food Matrix on the Gut Microbiota of Lean Humans and Humans with Obesity
Authors: Estrada-Sierra Nancy Abril , Gonzalez-Avila Marisela , Urias-Silvas Judith-Esmeralda , Rincon-Enriquez Gabriel , Garcia-Parra Maria Dolores , Villanueva-Rodriguez Socorro Josefina
Published: February 2024

Experimental studies have provided evidence that physicochemical interactions in the food matrix can modify the biologically beneficial effects of bioactive compounds, including their effect on gut microbiota. This work aimed to evaluate the effect of a food gel matrix with Opuntia ficus cladodes mucilage pectin and Citrus Aurantium extract on the growth of four beneficial gut bacteria obtained from the fecal microbiota of people who are lean or who have obesity after digestion in the upper digestive system. To accomplish this, a base formulation of Opuntia ficus cladodes mucilage with or without C. aurantium extract was submitted to an ex vivo fecal fermentation in an automatic and robotic intestinal system. The changes in the intestinal microbiota were determined by means of plate culture and 16S sequencing, while short-chain fatty acids (SCFA) produced in the colon were determined via gas chromatography. In the presence of the extract in formulation, greater growth of Bifidobacterium spp. (+1.6 Log10 Colonic Forming Unit, UFC) and Lactobacillus spp. (+2 Log10 UFC) in the microbiota of lean people was observed. Only the growth in Salmonella spp. (−1 Log10 UFC) from both microbiota was affected in the presence of the extract, which decreased in the ascending colon. SCFA was mainly produced by the microbiota of people who were lean rather than those who had obesity in the presence of the extract, particularly in the ascending colon. The effect of sour orange extract seems to depend on the origin of the microbiota, whether in people who have obesity (25 mM/L) or are lean (39 mM/L).
A First-Principle Study of Two-Dimensional Boron Nitride Polymorph with Tunable Magnetism
Authors: Qiao Liping , Ma Zhongqi , Yan Fulong , Wang Sake , Fan Qingyang
Published: February 2024

Using the first-principles calculation, two doping two-dimensional (2D) BN (boron nitride) polymorphs are constructed in this work. The two doping 2D BN polymorphs B5N6Al and B5N6C sheets are thermally stable under 500 K. All the B6N6, B5N6Al, and B5N6C sheets are semiconductor materials with indirect band gaps on the basis of a hybrid functional. The anisotropic calculation results indicate that Young’s modulus (E) and Poisson’s ratio (v) of the B6N6, B5N6Al, and B5N6C sheets are anisotropic in the xy plane. In addition, the magnetic properties of the B6N6, B5N6Al, and B5N6C sheets have also been investigated. According to the calculation of the magnetic properties, B6N6 sheet does not exhibit magnetism, while it shows weak magnetism after doping carbon atom to the BN sheet. This paper explores the influence mechanism of doping different atoms on the basic physical properties of two-dimensional BN sheets. It not only constructs a relationship between structure and performance but also provides theoretical support for the performance regulation of BN materials.
Association of Alkaline Phosphatase with Cardiovascular Disease in Patients with Dyslipidemia: A 6-Year Retrospective Study
Authors: Adamidis Petros Spyridonas , Florentin Matilda , Liberopoulos Evangelos , Koutsogianni Amalia Despoina , Anastasiou Georgia , Liamis George , Milionis Haralampos , Barkas Fotios
Published: February 2024

Background and Aim: Serum alkaline phosphatase (ALP) activity has been associated with atherosclerotic cardiovascular disease (ASCVD). We aimed to investigate the association of ALP with ASCVD in patients with dyslipidemia. Methods: We conducted a retrospective cohort study including consecutive adults with dyslipidemia followed-up for ≥3 years (from 1999 to 2022) in the outpatient Lipid Clinic of Ioannina University General Hospital, Greece. The primary endpoint was the association between baseline ALP and incident ASCVD after adjusting for traditional risk factors (i.e., sex, age, hypertension, diabetes, smoking, and dyslipidemia), baseline ASCVD, and lipid-lowering treatment. ALP levels were stratified by tertiles as follows: low: <67 U/L, middle: 67–79 U/L, high: ≥79 U/L. Results: Overall, 1178 subjects were included; 44% were males, and their median age was 57 years (range: 49–65). During a 6-year median follow-up (interquartile range: IQR: 4–9), 78 new ASCVD events (6.6%) occurred. A statistically significant association between baseline ALP levels and incident ASCVD was demonstrated (Odds Ratio, OR: 6.99; 95% Confidence Interval, CI: 2.29–21.03, p = 0.001). Subjects in the highest ALP tertile had the highest odds for ASCVD when compared with those in the lowest tertile (OR: 2.35; 95% CI: 1.24–4.41, p = 0.008). Conclusions: The present study indicates an association between ALP and the development of ASCVD in patients with dyslipidemia, which underscores the potential of ALP as a predictive tool or a therapeutic target in the realm of ASCVD prevention within this population.
An Innovative Topical Medical Device with Hyaluronic Acid and Polypeptides in Patients with Reduced Knee Function
Authors: Bonanzinga Tommaso , De Sensi Alice Giulia , Balzarini Beatrice , Doro Gian Luca , Bertolino Luca , Forte Luca , Kon Elizaveta
Published: February 2024

A topical medical device, AI500®, constituted of a single-chain polypeptide embedded in hyaluronic acid, was tested and evaluated in patients with reduced knee function due to osteoarthritis and other knee conditions. A total of 35 participants with reduced knee function assessed by the WOMAC Physical Function score were recruited. Four study visits were planned, from the first application at V0 to 1 week follow up at V3. Patient symptomatology was evaluated after 24 h (V1) and after 48 h (V2) through phone contact, and after 1 week from V0, on site (V3). The overall duration of the follow up was one week. An amelioration of 40% in WOMAC Physical Functional scores after 1 week of treatment was recorded, thus achieving the primary endpoint of 20%. Furthermore, a reduction of 29% in Physical Functional scores and of 28% in total WOMAC scores between V0-V2 was registered, together with a decrease of 39% between V0 and V3. The NRS scale showed a 29% and 37% reduction in pain between V0-V1 and V0-V2, respectively. Product safety was confirmed by the very low rate of adverse effects, non-device related, observed in only 2 patients out of 35, resolved spontaneously within 24–48 h. No safety concerns or risks associated with the use of the device were highlighted. There are few the studies on the topical use of HA-based gels for the treatment of knee problems. Compared to invasive intra-articular injections and oral pharmacological therapies used in cases of knee pain, the topical application of AI500® is non-invasive, safe, and appreciated by patients. Good results in terms of functional improvement and symptoms resolution were obtained in less than 1 week.