bluuebunny/crossref_metadata_2025 huggingface dataset:
Evaluation of the rbcL marker for metabarcoding of marine diatoms and inference of population structure of selected genera
Authors: Turk Dermastia Timotej , Vascotto Ivano , Francé Janja , Stanković David , Mozetič Patricija
Published: March 2023

Diatoms are one of the most important phytoplankton groups in the world’s oceans. There are responsible for up to 40% of the photosynthetic activity in the Ocean, and they play an important role in the silicon and carbon cycles by decoupling carbon from atmospheric interactions through sinking and export. These processes are strongly influenced by the taxonomic composition of diatom assemblages. Traditionally, these have been assessed using microscopy, which in some cases is not reliable or reproducible. Next-generation sequencing enabled us to study diversity in a high-throughput manner and uncover new distribution patterns and diversity. However, phylogenetic markers used for this purpose, such as various 18S rDNA regions, are often insufficient because they cannot distinguish between some taxa. In this work, we demonstrate the performance of the chloroplast-encoded rbcL marker for metabarcoding marine diatoms compared to microscopy and 18S-V9 metabarcoding using a series of monthly samples from the Gulf of Trieste (GoT), northern Adriatic Sea. We demonstrate that rbcL is able to detect more taxa compared to 18S-V9 metabarcoding or microscopy, while the overall structure of the diatom assemblage was comparable to the other two methods with some variations, that were taxon dependent. In total, 6 new genera and 22 new diatom species for the study region were identified. We were able to spot misidentification of genera obtained with microscopy such as Pseudo-nitzschia galaxiae, which was mistaken for Cylindrotheca closterium, as well as genera that were completely overlooked, such as Minidiscus and several genera from the Cymatosiraceae family. Furthermore, on the example of two well-studied genera in the region, namely Chaetoceros and particularly Pseudo-nitzschia, we show how the rbcL method can be used to infer even deeper phylogenetic and ecologically significant differences at the species population level. Despite a very thorough community analysis obtained by rbcL the incompleteness of reference databases was still evident, and we shed light on possible improvements. Our work has further implications for studies dealing with taxa distribution and population structure, as well as carbon and silica flux models and networks.
The DNA adenine methylase of Salmonella Enteritidis promotes their intracellular replication by inhibiting arachidonic acid metabolism pathway in macrophages
Authors: Wang Ming , Xiong Dan , Wang Xinwei , Gu Dan , Meng Chuang , Jiao Xinan , Pan Zhiming
Published: March 2023

Macrophages can participate in immune responses by altering their metabolism, and play important roles in controlling bacterial infections. However, Salmonella Enteritidis can survive and proliferate in macrophages. After the deletion of DNA adenine methylase (Dam), the proliferation of Salmonella Enteritidis in macrophages decreased, the molecular mechanism is still unclear. After infecting macrophages with Salmonella Enteritidis wild type and dam gene deletion strains, intracellular metabolites were extracted and detected by non-targeted metabolomics and fatty acid targeted metabolomics. We found Dam had significant effects on arachidonic acid and related metabolic pathways in macrophages. The dam gene can promote the proliferation of Salmonella Enteritidis in macrophages by inhibiting the metabolic pathway of cytosolic phospholipase A2-mediated arachidonic acid production and conversion to prostaglandin E2 in macrophages, reducing the secretion of the pro-inflammatory factors IL-1β and IL-6. In addition, inhibition of arachidonic acid-related pathways in macrophages by Arachidonyl trifluoromethyl ketone could restore the proliferation of dam gene deletion strains in macrophages. This study explored the role of Dam in the process of Salmonella Enteritidis invading host cells from the perspective of host cell metabolism, and provides new insights into the immune escape mechanism of Salmonella Enteritidis.
Effect of real-world fear on risky decision-making in medical school-based students: A quasi-experimental study
Authors: Wang Lei , Chen Sheng , Xiao Wei
Published: March 2023

Objective: To explore the effect of real-world fear on risky decision-making under certainty and uncertainty.

Methods: This quasi-experimental study enrolled non-psychology undergraduate volunteers aged between 17 and 20 years old from the Preventive Medical Institute medical school in Xi’an. Participants were randomly divided into two groups, and each group received a two-stage crossover design intervention (of a calm and fearful situation) and completed the tasks of risky decision-making under uncertainty (the balloon analog risk task: BART) and certainty (the Cambridge gambling task: CGT), respectively. The primary outcomes included the behavioral impulsivity measured by the BART value, and the speed of decision-making, the quality of decisions, the adventure index, behavioral impulsivity, and risk adjustment measured by CGT. The secondary outcome was the concentration of cortisol in the saliva.

Results: A total of 60 questionnaires and data were obtained from 60 participants (28 males and 32 females, aged 19.55 ± 0.75). Compared with the calm situation, participants were more likely to have a lower BART value (p = 0.013), slower speed of decision-making (p < 0.05), and higher adventure index (p = 0.018) in the fearful situation. The quality of decisions (p = 0.189), behavioral impulsivity index (p = 0.182), and risk adjustment (p = 0.063) between subjects in the fearful and calm situations were comparable. Furthermore, the mean value of the adventure index of CGT in male subjects was significantly higher than that in female subjects (p < 0.05), and the cortisol concentration in saliva during the fearful situation was significantly higher compared to the calm situation (p < 0.05).

Conclusion: Fear might reduce behavioral impulsivity under uncertainty, and increase the adventure index under certainty in risky decision-making. Risky behavior might be influenced by gender: under certainty in risky decision-making, men were more adventurous. Additionally, fear increased the secretion of cortisol in saliva.
Good multiple sclerosis (MS) care and how to get there in Canada: Perspectives of Canadian healthcare providers working with persons with MS
Authors: Petrin Julie , Marrie Ruth Ann , Devonshire Virginia , Jichici Draga , Hrebicek Olinka , Metz Luanne M. , Morrow Sarah A. , Oh Jiwon , Smyth Penelope , Donkers Sarah J.
Published: March 2023

Objective

The literature that has examined healthcare access and needs of the multiple sclerosis (MS) population is limited. Currently, no research has engaged healthcare providers delivering services to this population to examine their perspectives on the provision of MS care in Canada. We aimed to summarize what good MS care should look like according to Canadian healthcare providers working with people with MS, and to identify the supports and resources required, within their care setting, to enable this standard of care.

Methods

A qualitative descriptive approach was taken to analyze data from participants who responded to additional open-ended survey questions, within a larger “MS Models of Care Survey” targeting Canadian healthcare providers working with persons with MS.

Results

Currently, a gap exists between what healthcare providers working with persons with MS believe MS care should encompass and what they are able to offer. Participants emphasized that their MS clinics are currently understaffed and patient-to-provider ratios are high, leaving very little time to address the array of healthcare concerns their patients present with. The healthcare providers overwhelmingly described that moving toward multidisciplinary team-based MS care that includes appropriate numbers of MS-trained neurologists, nurses, physiotherapists, occupational therapists, and mental health providers working within one location would be their prioritized approach to comprehensively managing MS care. This model of care enables all professionals to effectively coordinate care and use their time efficiently by only focusing on their area of expertise, all while meeting the needs of their patient in one setting, reducing wait-times and improving overall care.

Conclusion

To meet the care needs of Canadians with MS, the healthcare system must consider standardizing and funding multidisciplinary team-based MS clinics, comparable to Stroke units, which continue to show favorable health outcomes after years of implementation.
The brain’s weakness in the face of trauma: How head trauma causes the destruction of the brain
Authors: Johnstone Daniel M. , Mitrofanis John , Stone Jonathan
Published: March 2023

Of all our organs, the brain is perhaps the best protected from trauma. The skull has evolved to enclose it and, within the skull, the brain floats in a protective bath of cerebrospinal fluid. It is becoming evident, however, that head trauma experienced in young adult life can cause a dementia that appears decades later. The level of trauma that induces such destruction is still being assessed but includes levels well below that which cracks the skull or causes unconsciousness or concussion. Clinically this damage appears as dementia, in people who played body-contact sports in their youth or have survived accidents or the blasts of combat; and appears also, we argue, in old age, without a history of head trauma. The dementias have been given different names, including dementia pugilistica (affecting boxers), chronic traumatic encephalopathy (following certain sports, particularly football), traumatic brain injury (following accidents, combat) and Alzheimer’s (following decades of life). They share common features of clinical presentation and neuropathology, and this conceptual analysis seeks to identify features common to these forms of brain injury and to identify where in the brain the damage common to them occurs; and how it occurs, despite the protection provided by the skull and cerebrospinal fluid. The analysis suggests that the brain’s weak point in the face of trauma is its capillary bed, which is torn by the shock of trauma. This identification in turn allows discussion of ways of delaying, avoiding and even treating these trauma-induced degenerations.
Case report: Ruxolitinib plus dexamethasone as first-line therapy in haemophagocytic lymphohistiocytosis
Authors: Zhao Lin , Yang Hui , Qu Wei-ying , Lu Ying-jia , Feng Zhou
Published: March 2023

Haemophagocytic lymphohistiocytosis (HLH) is a cytokine-driven inflammatory syndrome caused by uncontrolled hypersecretion of inflammatory cytokines. Conventional first-line treatment for HLH included HLH-94 and HLH-2004 regimens. However, quite a few patients do not respond to treatment or cannot tolerate intensive chemotherapy. We reported two cases of HLH, one caused by natural killer (NK)/T-cell lymphoma and another associated with missense variants in the perforin 1 gene. They both received the ruxolitinib plus dexamethasone protocol and had a rapid response to treatment without obvious adverse effects. Our report indicates that treatment with ruxolitinib plus dexamethasone might be a potential option for HLH, and clinical trials warrant further investigation. In addition, the detection of HLH-related genes is necessary for the identification of late-onset familial HLH in certain settings.
Transarterial arterial sclerosing embolization for the treatment of propranolol-resistant subglottic hemangioma: Feasibility and effificacy
Authors: Liu Zhuang , Song Dan , Wang Liang , Zhou Jie , Wang Changfeng , Li Jing , Sun Jiali , Zhang Xian , Guo Lei
Published: March 2023

Purpose

To assess the effectiveness and safety of transcatheter arterial sclerosing embolization (TASE) for the treatment of subglottic hemangiomas that did not respond appreciably to propranolol.

Materials and Methods

This study was a retrospective analysis. Of the 101 patients with subglottic hemangiomas admitted to our center, 10 (4 male and 6 female) patients were included in this study. All these patients underwent arterial embolization using Pingyangmycin and polyvinyl alcohol particles (300–500 μm). All patients were examined 1 month after the last treatment and monthly thereafter.

Results

TASE treatment was technically successful in all patients. Ten lesions were located in the subglottic region. The blood supply included the superior thyroid artery, brachiocephalic trunk, facial artery, and ascending pharyngeal arteries. The median maximal diameter of the hemangiomas significantly decreased from 8.5 mm before treatment to 2 mm after TASE (P <.05). The degree of laryngeal obstruction improved in all patients. No serious complications were noted. One patient developed fever postoperatively, and three patients had a mild cough.

Conclusions

For even subglottic hemangiomas with suboptimal efficacy of propranolol, TASE significantly reduced the size of hemangiomas with minimal adverse effects. It had a positive effect on the improvement of airway stenosis caused by subglottic hemangioma with poor effect of oral propranolol.
Chemopreventive effects of Xiang Sha Liu Jun Zi Tang on paclitaxel-induced leucopenia and neuropathy in animals
Authors: Shiah Her-Shyong , Lee Chia-Jung , Lee Fang-Yu , Tseng Sung-Hui , Chen Shih-Han , Wang Ching-Chiung
Published: March 2023

Paclitaxel frequently induces peripheral neuropathy and myelosuppression during cancer treatment. According to the National Health Insurance Research Database of Taiwan, traditional Chinese medicine doctors widely use Xiang Sha Liu Jun Zi Tang (XSLJZT) to treat breast cancer patients who have received paclitaxel. We explored the combined therapeutic effects of XSLZJT with paclitaxel. XSLJZT did not exhibit significant cytotoxic effects on P388-D1 cells; however, the combination of XSLJZT (100 and 500 mg/kg) with paclitaxel prolonged the survival rate in P388–D1 tumor-bearing mice compared to paclitaxel-only. In addition, XSLJZT was found to enhance white blood cells (WBC) counts and promote leukocyte rebound in paclitaxel-induced leukopenia in mice. XSLJZT also reduced paclitaxel-induced mechanical pain and inhibited c-Fos protein expression in the L4-6 spinal cords of Wistar rats. Moreover, paclitaxel-induced shortening of the nerve fibers of dorsal root ganglion cells was ameliorated by pre-treatment with XSLJZT. Therefore, we suggest that XSLJZT could be used as an adjunct for cancer patients, as the formula could decrease paclitaxel-induced neuropathy and myelosuppression.
Therapeutic potential of naringin in improving the survival rate of skin flap: A review
Authors: Wang Yincang , Li Xiaodong , Lv Hang , Sun Lin , Liu Bo , Zhang Xiaofeng , Xu Xilin
Published: March 2023

Naringin is the main component of Drynaria. Modern pharmacological studies have shown that naringin has a wide range of pharmacological activities, including antioxidant, anti-inflammatory, anti-apoptotic, anti-ulcer, and anti-osteoporosis effects. Its therapeutic effects have been observed in various clinical models, such as atherosclerosis, cardiovascular diseases, diabetes, neurodegenerative diseases, and rheumatic diseases. This review investigates the pharmacological effects of naringin and the associated mechanisms in improving flap survival. This review will also provide a reference for future rational application of naringin, especially in research to improve flap survival.
Sacubitril–valsartan versus enalapril for the treatment of acute decompensated heart failure in Chinese settings: A cost-effectiveness analysis
Authors: Hu Tianyang , Liu Yiting , Lou Yake
Published: March 2023

Background: The episode of acute decompensated heart failure (ADHF) is the main cause of hospitalization for heart failure (HF). Sacubitril–valsartan has been proven to be effective in reducing the risks of hospitalization for HF in ADHF. When to initiate sacubitril–valsartan in ADHF to make it the most cost-effective in China remains unclear.

Methods: A lifetime Markov model with a 1-month cycle length was developed to evaluate the cost-effectiveness of early or late initiation of sacubitril–valsartan versus enalapril in ADHF. Early initiation of sacubitril–valsartan meant that it was initiated after stabilization from ADHF, and late initiation of sacubitril–valsartan meant that it was initiated after stabilization from HF, which includes no hospitalization for at least three consecutive months. The primary outcome was the incremental cost-effectiveness ratio (ICER), expressed as the ratio of incremental cost to incremental effectiveness. The secondary outcomes were total costs and total effectiveness. Three times of per capita GDP of China in 2021 was set as the willingness-to-pay threshold. One-way sensitivity analysis and probabilistic sensitivity analysis were employed to test the robustness of the results.

Results: The early initiation of sacubitril–valsartan treatment resulted in an ICER of 3,662.4 USD per quality-adjusted life year, lower than the willingness-to-pay threshold, and the late initiation of sacubitril–valsartan treatment gained an ICER of 4,444.4 USD/QALY, still lower than the willingness-to-pay threshold. One-way sensitivity analysis showed that our results were robust, and probabilistic sensitivity analysis suggested that early initiation of sacubitril–valsartan in ADHF was cost-effective under a 97.4% circumstance.

Conclusion: Early initiation of sacubitril–valsartan after stabilization of ADHF is highly cost-effective compared with the use of enalapril; late initiation of sacubitril–valsartan after stabilization of HF is still cost-effective but not as cost-effective as early initiation of sacubitril–valsartan in ADHF. For Chinese ADHF patients, the time to initiate sacubitril–valsartan should be when the patient is stabilized from ADHF rather than when stabilized from HF, from the perspective of economic evaluation.
Investigating the searching behavior of Sino-U.S. relations in China based on complex network
Authors: Chen Jun , Wang Lei , Weng Wuyan
Published: March 2023

The Sino-U.S. relationship is one of the most important bilateral relationships in the literature of global geopolitics. Not only the two countries, but also other nations in the relevant regions have been influenced by their economic, cultural, political, educational, and diplomatic relations. In this paper, we have applied Visibility Graph as the method, analyzed the data from Baidu searching index of the keyword “Sino-U.S. relations” from 2011 to 2021 into a directionless and authoritarian network, and studied its dynamic characteristics. from the time series data, it has been found that the relationship between the data is closer with more edges, greater degrees, and greater clustering coefficients. Meanwhile, the shorter the average path length, the closer the relationship between the data. The results provide a new perspective for analyzing the time series characteristics of Sino-U.S. relations.
Adjustment and maladjustment to later life: Evidence about early experiences in the family
Authors: Alcaide Marta , Garcia Oscar F. , Queiroz Pablo , Garcia Fernando
Published: March 2023

Introduction

Previous parenting studies with European-American families have identified optimal parenting as being based on warmth combined with strictness (i.e., authoritative parenting). The present study analyzes, in adolescents and adults, their adjustment and maladjustment related to age and their early experiences in the family.

Methods

The sample consisted of 2,158 Spanish people (58.29% women): 624 adolescents, 630 young adults, 504 middle-aged adults, and 400 older adults. The families were classified into one of the four parental typologies (authoritative, indulgent, authoritarian, and neglectful) based on their scores in the two main dimensions (warmth and strictness). Child functioning was measured as components of adjustment (self-esteem, emotional self-concept, and empathy) and maladjustment (aggression and hostile sexism).

Results

Overall, only adolescents and adult children raised in indulgent families reported the highest self-esteem, emotional self-concept, and empathy, and the lowest aggression and hostile sexism. Authoritative parenting (warmth with strictness) was related to a lower emotional self-concept and greater aggression and hostile sexism than indulgent parenting (warmth without strictness). The worst scores corresponded to authoritarian and neglectful parenting.

Discussion

The present findings provide new evidence about early experiences in the family, even after parental socialization has ended. Interestingly, contrary to the main findings from classic studies with European-American families, only high parental warmth combined with low parental strictness (i.e., indulgent parenting) is always positive for greater adjustment and less maladjustment in all age groups.
Always look on the bright side of life: Individual differences in visual attentional breadth for understanding temperament and emotion regulation in adolescents
Authors: Van Beveren Marie-Lotte , Braet Jolien , De Raedt Rudi , Grol Maud , Wante Laura , Braet Caroline
Published: March 2023

Introduction

Cognitive-affective models of depression show that negative and positive emotionality differentially confer risk for depression through maladaptive and adaptive emotion regulation (ER) strategies respectively. Yet, no research has examined the mechanisms through which these temperament traits shape individual differences in ER. The current study explored the mediating role of attentional breadth for emotional information in the distinct pathways from temperament to ER strategies in adolescents.

Methods

The hypotheses were tested in a selected sample of 71 adolescents (M = 14.15, SD = 1.90; 62% girls) using a previously validated measure of visuospatial attentional breadth.

Results

First, positive emotionality was positively associated with attentional breadth for positive stimuli and temperamental vulnerable adolescents showed deficits in the processing of positive stimuli when presented far from the center of the visual field. Second, attentional breadth towards neutral stimuli was positively related to adaptive ER strategies. Third, no evidence was found for the proposed mediation models. However, post-hoc analyses provided preliminary evidence for a reversed mediation model in which adaptive ER strategies mediate the relationship between temperament and attentional breadth towards neutral stimuli.

Discussion

The results underscore the apparent complexity of the relations between temperament, attentional breadth, and ER and point out the need for further research in order to inform early intervention.
Students’ perceptions of the rules and restrictions of gender at school: A psychometric evaluation of the Gender Climate Scale (GCS)
Authors: Ullman Jacqueline , Hobby Lucy , Magson Natasha R. , Zhong Hua Flora
Published: March 2023

Research in the field of gender and sexuality diversity and, more specifically, negative attitudes toward gender and sexuality diverse individuals, has acknowledged the relationship between individuals’ endorsement of sex-differentiated, normative gender roles and their attitudes toward gender and sexuality diversity. Such work has highlighted how normative expectations of gender, drawn from binarized gender roles, sit at the heart of homophobic and transphobic attitudes. Previous research in high school settings has measured gender and sexuality diverse (GSD) students’ experiences of homo/transphobic harassment as an element of ‘school climate’ with regard to acceptance of gender and sexuality diversity. However, to date, no research has measured GSD students’ perceptions about how valued binarized, gender-normative roles are at their schools, or the ways in which these norms might impact, and potentially constrain, these students’ academic and social schooling lives. The aim of the present study was to address this gap by developing and testing a new, multidimensional measure (the Gender Climate Scale; GCS) of GSD students’ ideas about how gender norms function within their school. Using a convenience sample of 2,376 Australian high school students who identify as GSD, the GCS was evaluated for its reliability, construct, and criterion validity and measurement invariance using confirmatory factor analysis (CFA) methods. Findings revealed that the estimates produced from the GCS were reliable, valid, and invariant across student reported gender (male/female/non-binary) and location (urban/rural). Criterion validity was supported, with GCS factors representing the promotion of traditional gender roles in the schooling environment negatively associated with perceived school belonging and inclusion and positively associated with bullying and social isolation. Future research with the GCS can inform school and curriculum policy on this important measure of school climate, not just for GSD students but for whole student cohorts.
Brain activation in individuals suffering from bulimia nervosa and control subjects during sweet and sour taste stimuli
Authors: Armon Daphna Bardin , Bick Atira , Florentin Sharon , Laufer Sofia , Barkai Gabriel , Bachar Eytan , Hendler Talma , Bonne Omer , Keller Shikma
Published: March 2023

Introduction

Episodes of eating great quantities of extremely sweet and often aversive tasting food are a hallmark of bulimia nervosa. This unique eating pattern led researchers to seek and find differences in taste perception between patients and healthy control subjects. However, it is currently not known if these originate from central or peripheral impairment in the taste perception system. In this cross sectional study, we compare brain response to sweet and sour stimuli in 5 bulimic and 8 healthy women using functional magnetic resonance imaging (fMRI).

Materials and methods

Sweet, sour and neutral (colorless and odorless) taste solutions were presented to subjects while undergoing fMRI scanning. Data were analyzed using a block design paradigm.

Results

Between-group differences in brain activation in response to both sweet and sour tastes were found in 11 brain regions, including operculum, anterior cingulate cortex, midbrain, and cerebellum. These are all considered central to perception and processing of taste.

Conclusion

Our data propose that sweet and sour tastes may have reward or aversion eliciting attributes in patients suffering from bulimia nervosa not found in healthy subjects, suggesting that alteration in taste processing may be a core dysfunction in bulimia nervosa (BN).
Perceived stigma, common mental disorders and associated factors among primary caregivers of adult patients with mental illness attending at public hospitals, Harari regional state, Eastern Ethiopia: A multicenter cross-sectional study
Authors: Nigussie Kabtamu , Tesfaye Dejene , Bete Tilahun , Asfaw Henock
Published: March 2023

Background

Giving care to someone who is experiencing mental distress is a difficult and challenging task that could be detrimental to the caregiver's overall quality of life. Numerous studies show that caregivers frequently experience mental distress and feel stigmatized. However, there is limited study done on this topic in our country.

Objective

To determine the magnitude and associated factors of perceived stigma and common mental disorder among primary caregivers of adults with mental illness in public hospitals at Harari regional state, Eastern Ethiopia, 2022.

Methods

A cross-sectional study design was conducted. A total of 419 participants were recruited by a systematic random sampling technique. The data was collected by using a face-to-face interview and by reviewing patients' charts. Perceived stigma and common mental disorder were assessed by Family Interview Schedule and Self-Reporting Questionnaire, respectively. Epi-data version 4.6.2 was used to enter data, and SPSS version 20 was used to analyze. Bivariable and multivariable logistic regression were performed. P-values < 0.05 were considered statistically significant.

Result

The magnitude of perceived stigma and common mental disorder were 42.5% (95% CI, 37.7–47.3) and 39.4% (95% CI, 34.8–44.0) respectively. Age of caregiver between 26 and 33 [AOR = 3.13, 95% CI: (1.71–8.93)], no formal education [AOR = 3.85, 95% CI: (1.81–8.15)], illness duration ≥7 years [AOR = 1.93, 95% CI: (1.04–3.57)], family history of mental illness [AOR = 1.92, 95% CI: (1.09–3.39)] and poor social support [AOR = 4.87, 95% CI: (3.74–12.71)] were significantly associated with perceived stigma. Being female [AOR = 1.92, 95% CI: (1.31–3.34)], having no formal education [AOR = 4.04, 95% CI: (2.15–10.01)], having a family history of mental illness [AOR = 2.26, 95% CI: (1.29–4.00)], having comorbid other illness [AOR = 2.13, 95% CI: (1.15–3.94)], and having poor social support [AOR = 4.58, 95% CI: (2.53–8.28)] were significantly associated with common mental disorder.

Conclusion

The magnitude of perceived stigma and common mental disorder was high among primary caregivers of patients with mental illness compared with other studies. Age of caregiver between 26 and 33, no formal education, duration of illness ≥7 years, family history of mental illness and poor social support were associated with perceived stigma and being female, no formal education, family history of mental illness, comorbid other illness and poor social support were associated with common mental disorder.
Rehabilitation professionals' perspectives of factors influencing return to occupation for people with lower limb amputation in East, South, and Southeast Asian developing countries: A qualitative study
Authors: Sayeed Md Shapin Ibne , Oakman Jodi , Stuckey Rwth
Published: March 2023

Objective

To identify barriers and enablers for return to occupation (RTO) for people with lower limb amputation (LLA) in East, South, and Southeast Asian developing countries from the perspective of rehabilitation professionals.

Methods

A convenience sample of rehabilitation professionals working in Asian developing countries participated in online in-depth interviews between September 2021 and February 2022. Interview transcripts were analyzed and thematically coded to the modified Health Care Delivery System Approach (HCDSA) framework. COREQ guidelines were followed.

Results

Twenty-eight interviewees from 13 countries shared their experiences of factors related to RTO for people with LLA. Identified factors described barriers and facilitators for RTO at all four HCDSA framework levels. The “environmental” level had the most identified factors (n = 56) and the “care team” level the least (n = 31). Common environmental RTO challenges included cultural attitudes to women; lack of rural/remote services; inadequate numbers and regulation of rehabilitation professionals; inappropriate prosthesis; limited government support for rehabilitation, and reliance on charitable models.

Conclusions

Despite varied cultural, religious, and geographical characteristics, consistent factors impacting RTO were identified within these thirteen countries. Identified barriers to RTO underline the need for improvements throughout service systems from the acute-care focus on saving life without consideration of RTO, the rehabilitation focused primarily on mobility, to the lack of occupational rehabilitation services and supporting policy in these countries. These interlinked factors at different levels of healthcare service systems reinforce the importance of systems approaches to best utilize limited resources toward improving RTO in this region.
Undiagnosed diseases: Needs and opportunities in 20 countries participating in the Undiagnosed Diseases Network International
Authors: Taruscio Domenica , Salvatore Marco , Lumaka Aimè , Carta Claudio , Cellai Laura L. , Ferrari Gianluca , Sciascia Savino , Groft Stephen , Alanay Yasemin , Azam Maleeha , Baynam Gareth , Cederroth Helene , Cutiongco-de la Paz Eva Maria , Dissanayake Vajira Harshadeva Weerabaddana , Giugliani Roberto , Gonzaga-Jauregui Claudia , Hettiarachchi Dineshani , Kvlividze Oleg , Landoure Guida , Makay Prince , Melegh Béla , Ozbek Ugur , Puri Ratna Dua , Romero Vanessa , Scaria Vinod , Jamuar Saumya S. , Shotelersuk Vorasuk , Roccatello Dario , Gahl William A. , Wiafe Samuel A. , Bodamer Olaf , Posada Manuel
Published: March 2023

Introduction

Rare diseases (RD) are a health priority worldwide, overall affecting hundreds of millions of people globally. Early and accurate diagnosis is essential to support clinical care but remains challenging in many countries, especially the low- and medium-income ones. Hence, undiagnosed RD (URD) account for a significant portion of the overall RD burden.

Methods

In October 2020, the Developing Nations Working Group of the Undiagnosed Diseases Network International (DNWG-UDNI) launched a survey among its members, belonging to 20 countries across all continents, to map unmet needs and opportunities for patients with URD. The survey was based on questions with open answers and included eight different domains. Conflicting interpretations were resolved in contact with the partners involved.

Results

All members responded to the survey. The results indicated that the scientific and medical centers make substantial efforts to respond to the unmet needs of patients. In most countries, there is a high awareness of RD issues. Scarcity of resources was highlighted as a major problem, leading to reduced availability of diagnostic expertise and research. Serious equity in accessibility to services were highlighted both within and between participating countries. Regulatory problems, including securing informed consent, difficulties in sending DNA to foreign laboratories, protection of intellectual property, and conflicts of interest on the part of service providers, remain issues of concern. Finally, most respondents stressed the need to strengthen international cooperation in terms of data sharing, clinical research, and diagnostic expertise for URD patients in low and medium income countries.

Discussion

The survey highlighted that many countries experienced a discrepancy between the growing expertise and scientific value, the level of awareness and commitment on the part of relevant parties, and funding bodies. Country-tailored public health actions, including general syllabus of medical schools and of the education of other health professionals, are needed to reduce such gaps.
An evaluation of a computational technique for measuring the embeddedness of sustainability in the curriculum aligned to AASHE-STARS and the United Nations Sustainable Development Goals
Authors: Lemarchand Philippe , MacMahon Cormac , McKeever Mick , Owende Philip
Published: March 2023

Introduction

SDG 4.7 mandates university contributions to the United Nations (UN) Sustainable Development Goals (SDGs) through their education provisions. Hence, universities increasingly assess their curricular alignment to the SDGs. A common approach to the assessment is to identify keywords associated with specific SDGs and to analyze for their presence in the curriculum. An inherent challenge is associating the identified keywords as used in the diverse set of curricular contexts to relevant sustainability indicators; hence, the urgent need for more systematic assessment as SDG implementation passes its mid-cycle.

Method

In this study, a more nuanced technique was evaluated with notable capabilities for: (i) computing the importance of keywords based on the term frequency-inverse document frequency (TF-IDF) method; (ii) extending this computation to the importance of courses to each SDG and; (iii) correlating such importance to a statistical categorization based on the Association for the Advancement of Sustainability in Higher Education (AASHE) criteria. Application of the technique to analyze 5,773 modules in a university's curriculum portfolio facilitated categorization of the modules/courses to be “sustainability-focused” or “sustainability-inclusive.” With the strategic objective of systematically assessing the sustainability content of taught curricula, it is critical to evaluate the precision and accuracy of the computed results, in order to attribute text with the appropriate SDGs and level of sustainability embeddedness. This paper evaluates this technique, comparing its results against a manual and labor-intensive interpretation of expert informed assessment of sustainability embeddedness on a random sample of 306 modules/courses.

Results and discussion

Except for SDGs 1 and 17, the technique exhibited a reasonable degree of accuracy in predicting module/course alignment to SDGs and in categorizing them using AASHE criteria. Whilst limited to curricular contexts from a single university, this study indicates that the technique can support curricular transformation by stimulating enhancement and reframing of module/course contexts through the lens of the SDGs.
Prediction of Head Related Transfer Functions Using Machine Learning Approaches
Authors: Fernandez Martinez Roberto , Jimbert Pello , Sumner Eric Michael , Riedel Morris , Unnthorsson Runar
Published: March 2023

The generation of a virtual, personal, auditory space to obtain a high-quality sound experience when using headphones is of great significance. Normally this experience is improved using personalized head-related transfer functions (HRTFs) that depend on a large degree of personal anthropometric information on pinnae. Most of the studies focus their personal auditory optimization analysis on the study of amplitude versus frequency on HRTFs, mainly in the search for significant elevation cues of frequency maps. Therefore, knowing the HRTFs of each individual is of considerable help to improve sound quality. The following work proposes a methodology to model HRTFs according to the individual structure of pinnae using multilayer perceptron and linear regression techniques. It is proposed to generate several models that allow knowing HRTFs amplitude for each frequency based on the personal anthropometric data on pinnae, the azimuth angle, and the elevation of the sound source, thus predicting frequency magnitudes. Experiments show that the prediction of new personal HRTF generates low errors, thus this model can be applied to new heads with different pinnae characteristics with high confidence. Improving the results obtained with the standard KEMAR pinna, usually used in cases where there is a lack of information.
The Greenhouse Gas Crisis and the Logistic Growth Curve
Authors: Kolmes Steven A.
Published: March 2023

The greatest challenge of the coming century will be the consequences of an imbalanced atmosphere. Currently, projections of global heating due to an increasingly imbalanced atmosphere are dire, but they underestimate the near-term heating impacts of the growing concentrations of methane. Industrially mediated carbon capture and storage sometimes gets raised as a promising solution on the CO2 front, but it is presently commercially inviable. Despite these facts, we nonetheless need to act globally to reduce the atmospheric concentrations of greenhouse gases, although our increasingly separate information ecosystems make finding a way to express the reality of the atmospheric imbalance crisis to a wide audience daunting. One approach to presenting the atmospheric imbalances leading to global heating is to strip the discussion down initially to its bare bones with a sharp focus on the variables of the logistic growth equation. Although virtually anything can be politicized, the logistic growth equation’s variables are at least apolitical in their origin. After examining those variables, we can proceed to focus on density-dependent mortality factors (DDMFs) and their relationship to visible climatic changes driven by atmospheric imbalances. Both the Global North and the Global South need to do all that we do to reduce atmospheric greenhouse gas accumulation, reducing DDMFs, while paying careful attention to Indigenous rights and to the need for global gender equity, so that our efforts to control DDMFs do not produce a new expression of colonialism.
Survey of Distances between the Most Popular Distributions
Authors: Kelbert Mark
Published: March 2023

We present a number of upper and lower bounds for the total variation distances between the most popular probability distributions. In particular, some estimates of the total variation distances in the cases of multivariate Gaussian distributions, Poisson distributions, binomial distributions, between a binomial and a Poisson distribution, and also in the case of negative binomial distributions are given. Next, the estimations of Lévy–Prohorov distance in terms of Wasserstein metrics are discussed, and Fréchet, Wasserstein and Hellinger distances for multivariate Gaussian distributions are evaluated. Some novel context-sensitive distances are introduced and a number of bounds mimicking the classical results from the information theory are proved.
Methane, Ethylene, and Ethane Detection by Differential Helmholtz Resonance Spectroscopy Using a 3345 nm Mid-Infrared Tunable Diode Laser Source
Authors: Wu Zhe , Shi Yunxing , Han Yuwang
Published: March 2023

Gas detection based on photoacoustic spectroscopy (PAS) has attracted extensive attention due to its high sensitivity and large range of linearity. Herein, to achieve the simultaneous detection of the light carbon gases (CH4, C2H4, and C2H6), a gas detection system was constructed using a single mid-infrared tunable diode laser (central wavelength 3345 nm) source based on differential Helmholtz resonance spectroscopy (DHRS). The detection parameters (driving current, modulation depth, phase angle, etc.) were optimized under ambient pressure using the 1f demodulation method. With an integration time of 2 s, the detection limit reached 98.8 ppb, 252 ppb, and 33 ppb for methane, ethylene, and ethane, respectively. The validation test of the three-component mixture shows that the cross-interference can be effectively reduced by multiwavelength linear regression, and single wavelength linear regression causes large errors in the quantification of methane and ethylene.
A Brief Review of FT-IR Spectroscopy Studies of Sphingolipids in Human Cells
Authors: Faramarzi Bahar , Moggio Martina , Diano Nadia , Portaccio Marianna , Lepore Maria
Published: March 2023

In recent years, sphingolipids have attracted significant attention due to their pivotal role in cellular functions and physiological diseases. A valuable tool for investigating the characteristics of sphingolipids can be represented via FT-IR spectroscopy, generally recognized as a very powerful technique that provides detailed biochemical information on the examined sample with the unique properties of sensitivity and accuracy. In the present paper, some fundamental aspects of sphingolipid components of human cells are summarized, and the most relevant articles devoted to the FT-IR spectroscopic studies of sphingolipids are revised. A short description of different FT-IR experimental approaches adopted for investigating sphingolipids is also given, with details about the most commonly used data analysis procedures. The present overview of FT-IR investigations, although not exhaustive, attests to the relevant role this vibrational technique has played in giving significant insight into many aspects of this fascinating class of lipids.
Modeling and Complex Analysis of the Topology Parameters of Ventilation Networks When Ensuring Fire Safety While Developing Coal and Gas Deposits
Authors: Bosikov Igor Ivanovich , Martyushev Nikita V. , Klyuev Roman V. , Savchenko Irina A. , Kukartsev Vladislav V. , Kukartsev Viktor A. , Tynchenko Yadviga A.
Published: March 2023

Underground mining, including underground coal mining, is accompanied by accidents and fire hazards that pose a threat to the life safety of miners. The fire hazard increases with an increase in the mining depth. Currently, most accidents in coal mines are mine fires. The cost of eliminating mine fires is 80–95% of the cost of eliminating all accidents occurring at mining enterprises. Therefore, the problem of developing a new methodology for modeling the ventilation network parameters of the mine to increase the reliability of controlling the aerogas mode at the excavation site is very relevant. The comprehensive analysis and assessment of gas-dynamic processes in coalmines under study were carried out using the methods of probability theory and mathematical statistics. Spatial data were processed using spline interpolation in “gnuplot”. As a result, a generalized expression for the transfer functions of coalmine objects, taking into account delays, was developed, including the description of dynamic properties of mining sites under various operating modes. The principal possibility of using a graphical method for estimating additional parameters of the sections of the ventilation system branches has been proved due to the alignment of their profiles at an equivalent distance relative to an arbitrary analogue. The improved method of spatial modeling was used to determine the gas-dynamic characteristics through additive gas-dynamic processes. The studies have been carried out and the method for managing the process of changing connections between devices (controllers–switches) of the technical system was developed in order to obtain greater reliability for safe mining. In subsequent studies, there is an issue of more detailed clarification of the peculiarities concerning the interrelations between the studied parameters in several projections of the response space.
Prediction of Critical Heat Flux of Mixtures Flowing in Channels
Authors: Shah Mirza M.
Published: March 2023

Boiling of mixtures in channels is involved in many chemical processes, and refrigerant mixtures are finding a rapidly increasing use in the refrigeration industries. Hence, reliable prediction of CHF (critical heat flux) is needed. There is no published method that has been verified over a wide range of parameters. In this study, available data for CHF in tubes and annuli were compared to general correlations for tubes and annuli, which are well-verified for single-component fluids. This paper presents the results of this comparison. It was found that general correlations for single-component fluids were in satisfactory agreement with data for mixtures. The data included mixtures of refrigerants and chemicals, reduced pressure 0.02 to 0.8597, mass flux 200 to 3798 kgm−2s−1, temperature glide 2.1 to 21.9 K, and critical quality −0.46 to 0.99. The data for annuli were predicted with a MAD (mean absolute deviation) of 13.6%, and the data for tubes had a MAD of 13.9% when compared to the Shah correlations for single-component fluids. Based on the results of the present data analysis and results of research on pool boiling, a range is identified in which pure fluid correlations can be used for mixtures. This will be useful in the design of heat exchangers involving the boiling of mixtures in channels.