bluuebunny/crossref_metadata_2025 huggingface dataset:
Self-Incompatibility and Pollination Efficiency in Coffea canephora Using Fluorescence Microscopy
Authors: Silva Adriele Nunes Rodrigues , Rocha Rodrigo Barros , Teixeira Alexsandro Lara , Espindula Marcelo Curitiba , Partelli Fábio Luiz , Caixeta Eveline Teixeira
Published: July 2024

In nature, the ability to avoid self-fertilization has evolved to prevent the deleterious effects of inbreeding. However, under cultivation conditions, self-incompatibility can reduce the pollination efficiency of Coffea canephora. The objective of this study was to characterize the self-incompatibility expression of the most cultivated genotypes in Western Amazonia, to improve the management of this coffee plant. In vitro pollinations were conducted among 45 genotypes, and the development of pollen tubes was evaluated using fluorescence microscopy. Pollination efficiency was evaluated considering the allelic variability within a breeding population from an ideal condition of maximum genetic variability. Based on the compatibility response, the genotypes were organized into six groups: group I (24.4%), group II (31.1%), group III (24.4%), group IV (2.2%), group V (2.2%), and group VI (15.6%). The lower frequencies of groups IV, V, and VI were associated with the lower frequency of the rarest allelic forms in this breeding population (p = 0.36, q = 0.26, r = 0.29, and s = 0.10). The correspondence between allelic and genotypic frequencies indicates that this population is in Hardy–Weinberg equilibrium (HWE) for this trait. Considering the cultivation of 2 to 10 clones, the population studied showed intermediate pollination efficiency between an ideal HWE population with p = q = r = s = 0.25 and a population with the rarest allelic forms (p = 0.48, q = 0.32, r = 0.19, s = 0.01). Efficiency estimates were stabilized from the cultivation of five clones, indicating that cultivating a minimum number of clones should be considered. Theoretically, maximum pollination efficiency is achieved by representing all alleles in equal proportions, whereas in practice, farmers should ensure the cultivation of plants from different compatibility groups, without significant imbalances.
Intercontinental Migration Facilitates Continuous Occurrence of the Desert Locust Schistocerca gregaria (Forsk., 1775) in Africa and Asia
Authors: Feng Shiqian , Shi Shuai , Ullah Farman , Zhang Xueyan , Yin Yiting , Li Shuang , Nderitu John Huria , Ali Abid , Dong Yingying , Huang Wenjiang , Hu Gao , Zhang Zehua , Tu Xiongbing
Published: July 2024

The desert locust, Schistocerca gregaria (Forsk., 1775), stands as one of the most pervasive pests globally, inflicting extensive damage across Asia and Africa. Facilitated by intercontinental migration, the desert locust engages in population exchange between different source areas, perpetuating its widespread proliferation. Despite the wind being recognized as a key factor during migration events, elucidating its precise influence on intercontinental migration has remained elusive. In this study, we scrutinized monitoring data sourced from the FAO monitoring system, pinpointing 13 desert locust events featuring intercontinental migrations since 1967. From these events, four migration routes were summarized, traversing the Red Sea (RS-WE and RS-EW) and the northern Indian Ocean (IO-WE and IO-EW). Typically, RS-WE and IO-EW migrations occurred between December and March, whereas RS-EW and IO-WE migrations were observed from May to June and April to July, respectively. Our examination of wind field data spanning the past 15 years revealed that wind direction and speed facilitated intercontinental migrations. Furthermore, migration trajectory modeling indicated that desert locusts might exhibit migratory behavior both during the day and at night in the cases of RS-WE and RS-EW, with cross-oceanic migration potentially lasting for a week for IO-WE and IO-EW. In summary, our study identifies four migration routes for the intercontinental migration of the desert locust, providing crucial support for the scientific prediction of its occurrence and contributing to international food security efforts.
Optimization Strategies for Atari Game Environments: Integrating Snake Optimization Algorithm and Energy Valley Optimization in Reinforcement Learning Models
Authors: Sarkhi Sadeq Mohammed Kadhm , Koyuncu Hakan
Published: July 2024

One of the biggest problems in gaming AI is related to how we can optimize and adapt a deep reinforcement learning (DRL) model, especially when it is running inside complex, dynamic environments like “PacMan”. The existing research has concentrated more or less on basic DRL approaches though the utilization of advanced optimization methods. This paper tries to fill these gaps by proposing an innovative methodology that combines DRL with high-level metaheuristic optimization methods. The work presented in this paper specifically refactors DRL models on the “PacMan” domain with Energy Serpent Optimizer (ESO) for hyperparameter search. These novel adaptations give a major performance boost to the AI agent, as these are where its adaptability, response time, and efficiency gains start actually showing in the more complex game space. This work innovatively incorporates the metaheuristic optimization algorithm into another field—DRL—for Atari gaming AI. This integration is essential for the improvement of DRL models in general and allows for more efficient and real-time game play. This work delivers a comprehensive empirical study for these algorithms that not only verifies their capabilities in practice but also sets a state of the art through the prism of AI-driven game development. More than simply improving gaming AI, the developments could eventually apply to more sophisticated gaming environments, ongoing improvement of algorithms during execution, real-time adaptation regarding learning, and likely even robotics/autonomous systems. This study further illustrates the necessity for even-handed and conscientious application of AI in gaming—specifically regarding questions of fairness and addiction.
Effects of Congenital Adrenal Hyperplasia (CAH) and Biological Sex on Brain Size
Authors: Luders Eileen , Gaser Christian , Spencer Debra , Thankamony Ajay , Hughes Ieuan , Srirangalingam Umasuthan , Gleeson Helena , Hines Melissa , Kurth Florian
Published: July 2024

Congenital Adrenal Hyperplasia (CAH) has been reported to involve structural alterations in some brain regions. However, it remains to be established whether there is also an impact on the size of the brain as a whole. Here, we compiled the largest CAH sample to date (n = 53), matched pair-wise to a control group (n = 53) on sex, age, and verbal intelligence. Using T1-weighted brain scans, we calculated intracranial volume (ICV) as well as total brain volume (TBV), which are both common estimates for brain size. The statistical analysis was performed using a general linear model assessing the effects of CAH (CAH vs. controls), sex (women vs. men), and any CAH-by-sex interaction. The outcomes were comparable for ICV and TBV, i.e., there was no significant main effect of CAH and no significant CAH-by-sex interaction. However, there was a significant main effect of sex, with larger ICVs and TBVs in men than in women. Our findings contribute to an understudied field of research exploring brain anatomy in CAH. In contrast to some existing studies suggesting a smaller brain size in CAH, we did not observe such an effect. In other words, ICV and TBV in women and men with CAH did not differ significantly from those in controls. Notwithstanding, we observed the well-known sex difference in brain size (12.69% for ICV and 12.50% for TBV), with larger volumes in men than in women, which is in agreement with the existing literature.
Effect of Warming on Personality of Mosquitofish (Gambusia affinis) and Medaka Fish (Oryzias latipes)
Authors: Wang Rong , Yao Baohui , Tan Zhaoxian , Mao Chengjie , Ma Yonggui , Qu Jiapeng
Published: July 2024

Global warming may accelerate the process of biological invasions, and invasive species that can quickly adapt to new environments will have a negative impact on native species. Animal personalities have significant implications for ecology and evolution. However, few studies have simultaneously examined the combined effects of climate warming and biological invasions on native species. In this study, we hypothesized that temperature was positively correlated with personality, and invasive species had stronger personalities than native species. Accordingly, we established control (20 °C) and warming groups (20 °C, 25 °C, and 30 °C) to rear mosquitofish and medaka fish, individuals acclimatized to rearing temperatures for 7 days, then measured their personalities (sociability, exploration, novelty, and boldness). The results showed that individuals exhibited repeatable variation along the four behavioral axes across all temperature conditions, providing evidence for the presence of personalities. Significant positive correlations were found between each pair of behaviors, indicating the presence of behavioral syndrome. Sociability and exploration were most affected by temperature, showing increasing trends in sociability, exploration, and novelty in both invasive and native species with rising temperatures. Compared to medaka fish, mosquitofish exhibited higher exploration and lower sociability at elevated temperatures, while showing little change in boldness. Our results provide evidence that increased temperatures may promote biological invasions and pose a potential threat to the survival of native species. These findings are significant for understanding the complex impacts of climate change on ecosystems and for formulating effective biodiversity preservation strategies.
Antibiotic Resistance of Bacteria Isolated from Clinical Samples and Organs of Rescued Loggerhead Sea Turtles (Caretta caretta) in Southern Italy
Authors: Esposito Emanuele , Pace Antonino , Affuso Andrea , Oliviero Maria , Iaccarino Doriana , Paduano Gianluigi , Maffucci Fulvio , Fusco Giovanna , De Carlo Esterina , Hochscheid Sandra , Di Nocera Fabio
Published: July 2024

Antimicrobial resistance affects all environments, endangering the health of numerous species, including wildlife. Increasing anthropic pressure promotes the acquisition and dissemination of antibiotic resistance by wild animals. Sea turtles, being particularly exposed, are considered sentinels and carriers of potential zoonotic pathogens and resistant strains. Therefore, this study examined the antibiotic resistance profiles of bacteria isolated from loggerhead sea turtles hospitalised in a rescue centre of Southern Italy over a 9-year period. Resistance to ceftazidime, doxycycline, enrofloxacin, flumequine, gentamicin, oxytetracycline and sulfamethoxazole-trimethoprim was evaluated for 138 strains isolated from the clinical samples or organs of 60 animals. Gram-negative families were the most isolated: Vibrionaceae were predominant, followed by Shewanellaceae, Pseudomonadaceae, Enterobacteriaceae and Morganellaceae. These last three families exhibited the highest proportion of resistance and multidrug-resistant strains. Among the three Gram-positive families isolated, Enterococcaceae were the most represented and resistant. The opportunistic behaviour of all the isolated species is particularly concerning for diseased sea turtles, especially considering their resistance to commonly utilised antibiotics. Actually, the multiple antibiotic resistance was higher when the sea turtles were previously treated. Taken together, these findings highlight the need to improve antimicrobial stewardship and monitor antibiotic resistance in wildlife, to preserve the health of endangered species, along with public and environmental health.
Impact of Nutritional Tea Polyphenols on Growth, Feed Efficiency, Biochemical Traits, Antioxidant Capacity, Haematological Parameters and Immunity in Coho Salmon (Oncorhynchus kisutch)
Authors: Yu Hairui , Sattanathan Govindharajan , Yu Leyong , Li Lingyao , Xiao Yufang
Published: July 2024

To evaluate the impact of nutritional tea polyphenols (TPs) on body composition, growth, biochemical markers, antioxidant capacity, and hemato-immunological levels, a ten-week feeding experiment was carried out on coho salmon (Oncorhynchus kisutch, 180.51 ± 0.15 g). The control group was fed a basal diet; the T1, T2, T3, and T4 groups were fed 0.005%, 0.01%, 0.02%, and 0.04% TPs, respectively. These results demonstrate that adding TPs significantly (p < 0.05) increased the coho salmon fish’s weight gain (WG), relative growth rate (RGR), condition factor (CF), feed efficacy (FE), daily growth rate (DGR), and specific growth rate (SGR). There was no discernible difference in the body compositions of the treated TPs and the control group (p > 0.05). In addition, the T3 group showed a significant (p < 0.05) decrease in GPT, LDL, HDL, TC, and CAT. Fish given a 0.02% diet containing TPs had significantly lower levels of malondialdehyde (MDA) in their liver; yet, the TP-treated groups had higher levels of SOD and CAT than the control (p < 0.05). The data analysis shows a significant rise in lysozyme, respiratory burst activity, bactericidal activity, and blood hematological parameters in the 0.01–0.04% TP groups. According to these findings, TPs could be a useful dietary supplement for raising the antioxidant status, growth parameters, haemato-immunological response, and whole-body composition of coho salmon fish.
Comparison of Different Methods for Assaying the In Vitro Activity of Cefiderocol against Carbapenem-Resistant Pseudomonas aeruginosa Strains: Influence of Bacterial Inoculum
Authors: García-Rivera Celia , Sánchez-Bautista Antonia , Parra-Grande Mónica , Ricart-Silvestre Andrea , Ventero María Paz , Tyshkovska Iryna , Merino Esperanza , Rodríguez Díaz Juan Carlos
Published: July 2024

Carbapenem-resistant Pseudomonas aeruginosa infections represent a critical public health concern, highlighting the need for the development of effective antibiotics. Cefiderocol demonstrated potent in vitro activity against Pseudomonas aeruginosa, particularly in strains that are resistant to other drugs. However, concerns regarding the emergence of drug-resistant strains persist. This study, conducted with 109 carbapenem-resistant Pseudomonas aeruginosa strains from the Spanish Hospital (Dr. Balmis, Alicante). The study evaluated susceptibility to cefiderocol in comparison to alternative antibiotics and including their susceptibility to bacterial inoculum, while assessing various testing methods. Our findings revealed high susceptibility to cefiderocol against carbapenem-resistant strains, with only 2 of 109 strains exhibiting resistance. Comparative analysis demonstrated superiority of cefiderocol towards alternative antibiotics. Both the E-test and disk-diffusion methods showed 100% concordance with the microdilution method in classifying strains as susceptible or resistant. However, 4.6% (5/109) of disc zone diameters fell within the technical uncertainty zone, so the E-test technique was found to be more useful in routine clinical practice. Additionally, escalating bacterial inoculum correlated with decreases in vitro activity, so this parameter should be adjusted very carefully in in vivo studies. This study underscores cefiderocol’s potential as a therapeutic option for carbapenem-resistant Pseudomonas aeruginosa infections. However, the emergence of drug-resistant strains emphasizes the critical need for a wise use of antibiotics and a continuous monitoring of resistance to antibiotics. Based on our in vitro data, further investigation concerning the impact of bacterial inoculum on drug efficacy is warranted in order to detect resistance mechanisms and optimize treatment strategies, thereby mitigating the risk of resistance.
Experimental Investigations into Failures and Nonlinear Behaviors of Structural Membranes with Open Cuttings
Authors: Li Wenrui , Liu Ping , Tang Baijian , Kaewunruen Sakdirat
Published: July 2024

Reportedly, structural failures in membrane structures have occurred frequently, mostly originating from localized damage caused by intense loads on the membrane surface. It is thus necessary to investigate the nonlinear behaviors and load-carrying capacity of membranes with local damage. This study has conducted uniaxial tensile tests for membranes with a variety of original defects by using a specialized experimental setup and photogrammetry technique. The nonlinear relationship between the mechanical properties and the deforming angle of membranes, which portrays the principal axis, tensor, tensile stress, and position of the original defects, is investigated. The entire process of membrane failure has been recorded, and the strain and stress during each test specimen are compared. The new results indicate that the membranes exhibit predominantly elastic deformation before failure but surprisingly impart brittle fracture upon failure. Finally, a novel approach for estimating the load-bearing capacity of initially damaged membranes was proposed through the analysis of the load-bearing capacity of the damaged membranes under various conditions, positions, angles, and other influential factors.
Shape Optimization of Structures by Biological Growth Method
Authors: Bocko Jozef , Delyová Ingrid , Kostka Ján , Sivák Peter , Fiľo Milan
Published: July 2024

Structural element shape optimization based on the biological growth method is increasingly used nowadays. This method consists of two main methods: topological optimization (soft kill option—SKO) and shape optimization (computer-aided optimization—CAO). This paper presents the solution procedures for both shape optimization and topological optimization. In applying these methods, first of all, a certain stress norm must be established, where the most appropriate and most used criterion is the equivalent stress according to von Mises. The application of the mentioned optimization methods is illustrated by several examples. The aim was to compare the change in volume or mass and the maximum stress of the structural elements between the different designs: the initial design, the design after topological optimization, and the design after shape optimization.
Experimental and Theoretical Study on Anchorage Loss of Prestressed CFRP-Reinforced Concrete Beams
Authors: Liu Qinrui , Jiang Haozhe , Tao Guocheng , Zhuge Ping
Published: July 2024

To investigate the anchorage loss mechanism of externally prestressed CFRP tendons in concrete beams, this study introduces a novel theoretical calculation system (TCS) developed through both the finite element method (FEM) and experimental validation. Firstly, the FEM and the proposed TCS were employed based on the mechanism of anchorage loss to compute the deformation of each part of the prestressed tendon–main beam connection system, ensuring result accuracy through mutual validation. Subsequently, field tests, designed according to FEM guidelines, measured the anchorage loss in externally prestressed CFRP tendons, with long-term monitoring included. Finally, experimental data were then used to refine the TCS. The results indicate that deformation at the connecting screw and the front end of the steel reaction frame constitutes approximately 95% of the total deformation, with theoretical calculations aligning closely with the FEM results. The field tests revealed that the anchorage loss of a 12 m long CFRP tendon under 950 MPa prestress accounted for about 35% of the total prestress loss. The discrepancy in deformation compared with the theoretical results was due to a gap of approximately 0.4 mm between the two threaded connections, which can be minimized by improving construction techniques. After correction, the calculation error was reduced to about 5%. Control variable studies confirmed that anchorage loss is influenced by the prestress level, the dimensions of the steel reaction frame front end, the connecting screw length, and the number of thread gaps. This study provides a comprehensive approach for accurately predicting and mitigating anchorage loss in externally prestressed CFRP tendons, with significant implications for future engineering applications.
Driving Behavior Characteristics of Merging Sections in the Urban Underground Road Junction: A Driving Simulator Study
Authors: Jeong Seungwon , Lee Dongmin
Published: July 2024

This study aims to investigate left- and right-side merging sections on urban underground roads based on virtual reality driving simulator experiments. The behaviors investigated were changed by acceleration lane in the merging section, including 100, 120, and 140 m, considering current design guidelines. Typically, lane changing behavior was studied based on experiments using speed and lateral placement on driving. The behavior of more speed reduction in merging sections occurred in left-side merging than in right-side merging sections. In the left-side merging sections, speed reduction and acceleration rate decreased with the length of the acceleration lane. In the cases with relatively long acceleration lanes, lane changing locations for left-side merging sections were more sensitive than those of right-side merging sections. Some results from the driving simulator experiments show that road geometric design based on left-side merging sections might have more risk situations due to driver expectation and behaviors. This article provides technical knowledge to be applied to the acceleration lanes of left-side merging sections that extend 1.4 times longer than the usual road designs.
Prediction of Accident Risk Levels in Traffic Accidents Using Deep Learning and Radial Basis Function Neural Networks Applied to a Dataset with Information on Driving Events
Authors: Arciniegas-Ayala Cristian , Marcillo Pablo , Valdivieso Caraguay Ángel Leonardo , Hernández-Álvarez Myriam
Published: July 2024

A complex AI system must be worked offline because the training and execution phases are processed separately. This process often requires different computer resources due to the high model requirements. A limitation of this approach is the convoluted training process that needs to be repeated to obtain models with new data continuously incorporated into the knowledge base. Although the environment may be not static, it is crucial to dynamically train models by integrating new information during execution. In this article, artificial neural networks (ANNs) are developed to predict risk levels in traffic accidents with relatively simpler configurations than a deep learning (DL) model, which is more computationally intensive. The objective is to demonstrate that efficient, fast, and comparable results can be obtained using simple architectures such as that offered by the Radial Basis Function neural network (RBFNN). This work led to the generation of a driving dataset, which was subsequently validated for testing ANN models. The driving dataset simulated the dynamic approach by adding new data to the training on-the-fly, given the constant changes in the drivers’ data, vehicle information, environmental conditions, and traffic accidents. This study compares the processing time and performance of a Convolutional Neural Network (CNN), Random Forest (RF), Radial Basis Function (RBF), and Multilayer Perceptron (MLP), using evaluation metrics of accuracy, Specificity, and Sensitivity-recall to recommend an appropriate, simple, and fast ANN architecture that can be implemented in a secure alert traffic system that uses encrypted data.
The Magnetic Levitation Weaving Needle Monitoring System and Predictive Analysis Based on Digital Twin
Authors: Wang Zixuan , Zhang Chi , Yin Aijie , Rao Cancan , Lin Peng , Zhang Chengjun , Wang Jun
Published: July 2024

To address the issues of low visibility, poor real-time performance, and weak interaction capabilities in the operation of magnetic levitation circular knitting needles, an online monitoring method based on digital twin technology for magnetic levitation knitting needles is proposed. This method first conducts an in-depth analysis of the digital twin five-dimensional model of the magnetic levitation knitting needle to monitor the operational status of the equipment in real time. Additionally, an online monitoring system based on the Unity3D engine and a digital twin-based architecture for magnetic levitation knitting needles are designed. On this basis, the comprehensive monitoring, operation, and maintenance of the magnetic levitation knitting needles are achieved through the design of virtual models, visual interfaces, and real-time data acquisition and driving technology. Finally, this method takes a 5-inch 32-needle magnetic levitation circular knitting machine as the application research object. By analyzing the movement trajectory and real-time current feedback data of the three working positions of the magnetic levitation knitting needles, the rationality, effectiveness, and accuracy of the proposed method and model are demonstrated, achieving online monitoring of the magnetic levitation knitting needles and reflecting the full lifecycle process of the magnetic levitation knitting needles.
Fungal Proteins: Sources, Production and Purification Methods, Industrial Applications, and Future Perspectives
Authors: Pobiega Katarzyna , Sękul Joanna , Pakulska Anna , Latoszewska Małgorzata , Michońska Aleksandra , Korzeniowska Zuzanna , Macherzyńska Zuzanna , Pląder Michał , Duda Wiktoria , Szafraniuk Jakub , Kufel Aniela , Dominiak Łukasz , Lis Zuzanna , Kłusek Emilia , Kozicka Ewa , Wierzbicka Anna , Trusińska Magdalena , Rybak Katarzyna , Kot Anna M. , Nowacka Małgorzata
Published: July 2024

In recent years, there has been an increasing demand for new sources of protein, both for human and animal nutrition. In addition to alternative sources of protein, such as algae or edible insects, protein obtained from yeast and mold biomass is becoming more and more important. The main fungal protein producers are the yeasts Saccharomyces cerevisiae, Kluyveromyces marxianus, Candida utilis, Yarrowia lipolytica, and the molds Fusarium venenatum, Aspergillus oryzae, and Monascus purpureus. The production of fungal protein has many advantages, including the ability to regulate the amino acid composition, high protein content in dry matter, the possibility of production in a continuous process, independence from climatic factors, and the possibility of using waste substrates as ingredients of media. One of the disadvantages is the high content of nucleic acids, which generates the need for additional purification procedures before use in food. However, a number of enzymatic, chemical, and physical methods have been developed to reduce the content of these compounds. The paper presents the current state of knowledge about fungal producers, production and purification methods, the global market, as well as opportunities and challenges for single-cell protein (SCP) production.
Reversible Data Hiding Algorithm in Encrypted Images Based on Adaptive Median Edge Detection and Matrix-Based Secret Sharing
Authors: Jiang Zongbao , Zhang Minqing , Dong Weina , Jiang Chao , Di Fuqiang
Published: July 2024

Reversible data hiding in encrypted images (RDH-EI) schemes based on secret sharing have emerged as a significant area of research in privacy protection. However, existing algorithms have limitations, such as low embedding capacity and insufficient privacy protection. To address these challenges, this paper proposes an RDH-EI scheme based on adaptive median edge detection (AMED) and matrix-based secret sharing (MSS). The algorithm creatively leverages the AMED technique for precise image prediction and then integrates the (r, n)-threshold MSS scheme to partition the image into n encrypted images. Simultaneously, it embeds identifying information during segmentation to detect potential attacks during transmission. The algorithm allows multiple data hiders to embed secret data independently. Experimental results demonstrate that the proposed algorithm significantly enhances the embedding rate while preserving reversibility compared to current algorithms. The average maximum embedding rates achieved are up to 5.8142 bits per pixel (bpp) for the (3, 4)-threshold scheme and up to 7.2713 bpp for the (6, 6)-threshold scheme. With disaster-resilient features, the algorithm ensures (n−r) storage fault tolerance, enabling secure multi-party data storage. Furthermore, the design of the identifying information effectively evaluates the security of the transmission environment, making it suitable for multi-user cloud service scenarios.
Potential of Portuguese Viticulture By-Products as Natural Resources of Bioactive Compounds—Antioxidant and Antimicrobial Activities
Authors: Vinha Ana F. , Sousa Carla , Vilela Andreia , Ferreira Joana , Medeiros Rui , Cerqueira Fátima
Published: July 2024

Vitis vinifera is the grape variety used in the production of wine and other products. In the wine production process, many of the vine’s by-products are wasted, namely seeds and stems. Given the proportion of wine production worldwide, the quantity of by-products is beginning to be an environmental problem, making it urgent to take measures for their use to obtain bioactive compounds with health benefits. The aim of this work was to study the antioxidant and antimicrobial activities of extracts from the seeds and stems of four Portuguese grape varieties: Touriga Franca, Touriga Nacional, Viosinho, and Tinta Roriz. Total phenolic (TPC) and total flavonoids (TFC) contents present in the different extracts were evaluated, as well as the antioxidant activity, by DPPH and FRAP methods. TPC and TFC values of the stem’s extracts are much higher than those of the seeds of the same grape variety in the same solvent. The antioxidant activity of aqueous and ethanolic stem extracts is higher than that obtained for the seeds, showing that antioxidant activity is related to the content of polyphenols. The antimicrobial activity of different stem and seed extracts was determined against yeasts and Gram-positive and Gram-negative bacteria, and the effect was determined based on the minimal inhibitory concentrations calculated (MIC). In general, the ethanol:water (1:1) extract of the seeds from the different varieties tested inhibited C. albicans ATCC10231 and C. krusei ATCC6258 growth even at 200 μg/mL, and the effect was fungicidal at 200 μg/mL. The same type of extract showed selective antimicrobial activity, inhibiting S. aureus ATCC29213 growth but having no effect against E. coli ATCC25922 even at 200 μg/mL. The effect against S. aureus was bactericidal (at 200 μg/mL) for Touriga Franca, Touriga Nacional, and Viosinho. Taking all these results into account, it can be concluded that the by-products of the grape varieties tested are important sources of bioactive products, particularly as antioxidants and antimicrobials.
A Crystal Plasticity-Based Simulation to Predict Fracture Initiation Toughness of Reactor-Grade Aluminium: Experimental Verification and Study of Effect of Crystal Orientation
Authors: Samal Mahendra Kumar , Sahu Trishant , Syed Ather
Published: July 2024

Aluminium alloys are used for the fabrication of the fuel clad of research-grade nuclear reactors as well as for several types of core components of high-flux research reactors. In order to carry out design and safety analysis of these components, their mechanical and fracture properties are required by the designer. In this work, experiments have been conducted on tensile specimens machined from an aluminium alloy block to evaluate the material stress-strain curve. Experiments have also been conducted on disc-shaped compact tension specimens in order to determine the fracture toughness of aluminium alloy. Numerical simulations of both tensile and fracture specimens have been carried out using the crystal plasticity model. Initially, the slip system level parameters of the crystal plasticity material model have been calibrated using experimental stress-strain data for single as well as polycrystalline aluminium. For the prediction of crack initiation toughness, Rice and Tracey’s damage model has been used. The critical damage parameter has been evaluated for a fractured specimen with a crack length-to-width (a/W) ratio of 0.6. The attainment of the critical damage parameter in the analysis corresponds to the instance of experimentally observed ductile crack initiation in the specimen. Later, this model was applied to other fracture specimens with different a/W ratios with values ranging from 0.39 to 0.59. It was observed that the critical damage parameter corresponding to crack initiation in the material has a very small variation, even if the specimens have different crack lengths. It is well-known in the literature that Rice and Tracey’s critical damage parameter is a material constant. Hence, we have applied the same model to predict crack initiation for single crystal fracture specimens with two different orientations of the crack plane. It was observed that the <111> orientation is more susceptible to crack initiation and propagation compared with the <100> orientation, as the damage parameter is high in the ligament of the specimen ahead of the crack tip for the same level of applied loading. As the [111] crack plane is more closely packed compared with the [100] plane, the distance between atomic planes is greater for the former, and hence, it is more susceptible to ductile damage. The results of the experiments and the material damage parameter are helpful for the integrity analysis of the fuel clad of research reactors as well as components of high-flux research reactors.
Italian National Radon Action Plan: New Challenges for Risk Assessment
Authors: La Verde Giuseppe , Della Peruta Giuseppe , Imparato Chiara , Ambrosino Fabrizio , Mottareale Rocco , Gagliardo Gaetano , Pugliese Mariagabriella
Published: July 2024

Radon gas is one of the chemical pollutants with one of the most significant physical effects due to its impact on human health: it is a radioactive noble gas which, if inhaled, can stochastically induce lung cancer. For this reason, it is classified as a category A substance and is the second cause of cancer after tobacco smoking. The monitoring and management of indoor radon is based on international recommendations but also national regulations, which, in recent years, have been updated by lowering the reference levels. In this work, some radon activity concentration data were evaluated by comparing the criteria of old and new legislation to highlight how the radiation protection approach has completely changed. Specifically, this study focuses on measurements in Campania, which, due to its originally volcanic geological structure, requires crucial attention in the context of radon risk assessment, given the considerable number of dwellings built in tuff. This initial data processing enabled the identification of potential high-priority radon risk areas, serving as an important reference point for the extension of the monitoring activities in Campania.
Production of Reverse Transcriptase and DNA Polymerase in Bacterial Expression Systems
Authors: Hriňová Kristína , Dlapová Johana , Kubala Bohuš , Kormanová Ľubica , Levarski Zdenko , Struhárňanská Eva , Turňa Ján , Stuchlík Stanislav
Published: July 2024

DNA amplification and reverse transcription enzymes have proven to be invaluable in fast and reliable diagnostics and research applications because of their processivity, specificity, and robustness. Our study focused on the production of mutant Taq DNA polymerase and mutant M-MLV reverse transcriptase in the expression hosts Vibrio natriegens and Escherichia coli under various expression conditions. We also examined nonspecific extracellular production in V. natriegens. Intracellularly, M-MLV was produced in V. natriegens at the level of 11% of the total cell proteins (TCPs) compared with 16% of TCPs in E. coli. We obtained a soluble protein that accounted for 11% of the enzyme produced in V. natriegens and 22% of the enzyme produced in E. coli. Taq pol was produced intracellularly in V. natriegens at the level of 30% of TCPs compared with 26% of TCPs in E. coli. However, Taq pol was almost non-soluble in E. coli, whereas in V. natriegens, we obtained a soluble protein that accounted for 23% of the produced enzyme. We detected substantial extracellular production of Taq pol. Thus, V. natriegens is a suitable alternative host with the potential for production of recombinant proteins.
Association between Elastic Modulus of Foot Soft Tissues and Gait Characteristics in Young Individuals with Flatfoot
Authors: Jiao Xin , Hu Tianyi , Li Yongjin , Wang Binbin , Acquah Mirabel Ewura Esi , Wang Zengguang , Chen Qianqian , Gan Yaokai , Gu Dongyun
Published: July 2024

Flatfoot is a common foot deformity, causing foot pain, osteoarthritis of the midfoot, and even knee and hip dysfunction. The elastic modulus of foot soft tissues and its association with gait biomechanics still remain unclear. For this study, we recruited 20 young individuals with flatfoot and 22 age-matched individuals with normal foot arches. The elastic modulus of foot soft tissues (posterior tibial tendon, flexor digitorum brevis, plantar fascia, heel fat pad) was obtained via ultrasound elastography. Gait data were acquired using an optical motion capture system. The association between elastic modulus and gait data was analyzed via correlation analysis. The elastic modulus of the plantar fascia (PF) in individuals with flatfoot was higher than that in individuals with normal foot arches. There was no significant difference in the elastic modulus of the posterior tibial tendon (PTT), the flexor digitorum brevis (FDB), or the heel fat pad (HFD), or the thickness of the PF, PTT, FDB, and HFD. Individuals with flatfoot showed greater motion of the hip and pelvis in the coronal plane, longer double-support phase time, and greater maximum hip adduction moment during walking. The elastic modulus of the PF in individuals with flatfoot was positively correlated with the maximum hip extension angle (r = 0.352, p = 0.033) and the maximum hip adduction moment (r = 0.429, p = 0.039). The plantar fascia is an important plantar structure in flatfoot. The alteration of the plantar fascia’s elastic modulus is likely a significant contributing factor to gait abnormalities in people with flatfoot. More attention should be given to the plantar fascia in the young population with flatfoot.
Recent Advances in Hydrogel-Based 3D Bioprinting and Its Potential Application in the Treatment of Congenital Heart Disease
Authors: Salih Tasneem , Caputo Massimo , Ghorbel Mohamed T.
Published: July 2024

Congenital heart disease (CHD) is the most common birth defect, requiring invasive surgery often before a child’s first birthday. Current materials used during CHD surgery lack the ability to grow, remodel, and regenerate. To solve those limitations, 3D bioprinting is an emerging tool with the capability to create tailored constructs based on patients’ own imaging data with the ability to grow and remodel once implanted in children with CHD. It has the potential to integrate multiple bioinks with several cell types and biomolecules within 3D-bioprinted constructs that exhibit good structural fidelity, stability, and mechanical integrity. This review gives an overview of CHD and recent advancements in 3D bioprinting technologies with potential use in the treatment of CHD. Moreover, the selection of appropriate biomaterials based on their chemical, physical, and biological properties that are further manipulated to suit their application are also discussed. An introduction to bioink formulations composed of various biomaterials with emphasis on multiple cell types and biomolecules is briefly overviewed. Vasculogenesis and angiogenesis of prefabricated 3D-bioprinted structures and novel 4D printing technology are also summarized. Finally, we discuss several restrictions and our perspective on future directions in 3D bioprinting technologies in the treatment of CHD.
Evaluation of Patellar Tendon Structural Changes following Biological Treatments: Secondary Analysis of Double-Blinded Clinical Trial of Bone Marrow Mesenchymal Stromal Cells and Leukocyte-Poor Platelet-Rich Plasma
Authors: Ortega-Cebrián Silvia , Soler-Rich Robert , Orozco Lluis , Rodas Gil
Published: July 2024

Objective quantification of tendon structural changes through imaging is only achieved by evaluating tendon structure using ultrasound tissue characterization (UTC) technology. This study compares the effects of bone marrow mesenchymal stromal cells (BM-MSC) and leukocyte-poor platelet-rich plasma (Lp-PRP) on tendon structure and clinical outcomes in male patients with patellar tendinopathy measured with UTC at 3, 6, and 12 months after treatment. This is a double-blinded clinical trial with a randomized active control study with 20 male patients diagnosed with patellar tendinopathy who underwent BM-MSC and Lp-PRP. Bilateral ultrasound tissue characterization scans of the patellar tendon were carried out after 3, 6, and 12 months, as well as tests for strength and pain. UTC patellar tendon was analyzed at the insertion, proximal, and mid-tendon. BM-MSC showed a greater capacity to promote further positive changes than Lp-PRP. Lp-PRP presented higher disorganized echo-type II in the mid-tendon (p = 0.04; ES = 1.06) and III (p = 0.02; ES = −1.47) after 3 months in the Lp-PRP group. Similar results were seen after 6 and 12 months. Pain and strength data show improvement in the treated tendon. BM-MSC treatment demonstrates a superior capacity to promote tendon regeneration and organization, restore strength, and reduce pain compared to Lp-PRP, after 3, 6, and 12 months in male patients with patellar tendinopathy.
Exploring Synergistic Effects of Bioprinted Extracellular Vesicles for Skin Regeneration
Authors: Taghdi Manal Hussein , Muttiah Barathan , Chan Alvin Man Lung , Fauzi Mh Busra , Law Jia Xian , Lokanathan Yogeswaran
Published: July 2024

Regenerative medicine represents a paradigm shift in healthcare, aiming to restore tissue and organ function through innovative therapeutic strategies. Among these, bioprinting and extracellular vesicles (EVs) have emerged as promising techniques for tissue rejuvenation. EVs are small lipid membrane particles secreted by cells, known for their role as potent mediators of intercellular communication through the exchange of proteins, genetic material, and other biological components. The integration of 3D bioprinting technology with EVs offers a novel approach to tissue engineering, enabling the precise deposition of EV-loaded bioinks to construct complex three-dimensional (3D) tissue architectures. Unlike traditional cell-based approaches, bioprinted EVs eliminate the need for live cells, thereby mitigating regulatory and financial obstacles associated with cell therapy. By leveraging the synergistic effects of EVs and bioprinting, researchers aim to enhance the therapeutic outcomes of skin regeneration while addressing current limitations in conventional treatments. This review explores the evolving landscape of bioprinted EVs as a transformative approach for skin regeneration. Furthermore, it discusses the challenges and future directions in harnessing this innovative therapy for clinical applications, emphasizing the need for interdisciplinary collaboration and continued scientific inquiry to unlock its full therapeutic potential.
In Search for a Pathogenesis of Major Depression and Suicide—A Joint Investigation of Dopamine and Fiber Tract Anatomy Focusing on the Human Ventral Mesencephalic Tegmentum: Description of a Workflow
Authors: Zielinski Jana M. , Reisert Marco , Sajonz Bastian E. A. , Teo Shi Jia , Thierauf-Emberger Annette , Wessolleck Johanna , Frosch Maximilian , Spittau Björn , Leupold Jochen , Döbrössy Máté D. , Coenen Volker A.
Published: July 2024

Major depressive disorder (MDD) is prevalent with a high subjective and socio-economic burden. Despite the effectiveness of classical treatment methods, 20–30% of patients stay treatment-resistant. Deep Brain Stimulation of the superolateral branch of the medial forebrain bundle is emerging as a clinical treatment. The stimulation region (ventral tegmental area, VTA), supported by experimental data, points to the role of dopaminergic (DA) transmission in disease pathology. This work sets out to develop a workflow that will allow the performance of analyses on midbrain DA-ergic neurons and projections in subjects who have committed suicide. Human midbrains were retrieved during autopsy, formalin-fixed, and scanned in a Bruker MRI scanner (7T). Sections were sliced, stained for tyrosine hydroxylase (TH), digitized, and integrated into the Montreal Neurological Institute (MNI) brain space together with a high-resolution fiber tract atlas. Subnuclei of the VTA region were identified. TH-positive neurons and fibers were semi-quantitatively evaluated. The study established a rigorous protocol allowing for parallel histological assessments and fiber tractographic analysis in a common space. Semi-quantitative readings are feasible and allow the detection of cell loss in VTA subnuclei. This work describes the intricate workflow and first results of an investigation of DA anatomy in VTA subnuclei in a growing naturalistic database.
Coevolution Mechanism of Remanufacturer–Construction Enterprise–Public in Construction and Demolition Waste Resource Utilization Projects under Green Value Co-Creation
Authors: Yi Beiyu , Zou Yuxi , Chen Weihong , Li Zihan , He Yuman , Jin Zican , Zhang Hao , Li Xingwei
Published: July 2024

The utilization of resources plays a crucial role in mitigating the environmental pollution issue that improper disposal of construction and demolition waste (CDW) causes. However, the slow growth of the recycled building materials market limits the development of CDW resource utilization. Green value co-creation among remanufacturers, construction enterprises, and the public in CDW resource utilization projects is an effective way to address the issue. This study, based on the theory of value co-creation, uses the evolutionary game method to construct an evolutionary game model for CDW resource utilization projects. The main conclusions are as follows: (1) When the degree of green value co-creation is 0.1 or 0.5, the remanufacturer, the construction enterprise, and the public cannot maintain a state of green value co-creation; when the degree of green value co-creation is 0.9, the remanufacturer, the construction enterprise, and the public in the CDW resource utilization project finally reach a stable state of green value co-creation. (2) When the degree of green value co-creation is 0.5, enhancing the green value co-creation willingness of the remanufacturer or the public can lead other CDW resource utilization project stakeholders to participate in green value co-creation. This study contributes to the promotion of stakeholder cooperation in CDW resource utilization projects, thus providing implications for the promotion of CDW resources.