bluuebunny/crossref_metadata_2025 huggingface dataset:
The Role of the Endometrial Microbiota in Endometrial Cancer: A Systematic Review of the Literature
Authors: Stabile Guglielmo , Doria Alessandra , Bruno Matteo , D’Indinosante Marco , Gallotta Valerio , Fanfani Francesco , Scambia Giovanni , Restaino Stefano , Vizzielli Giuseppe , Carlucci Stefania , Nappi Luigi
Published: November 2024

Background: Endometrial cancer is currently the sixth most frequent cancer in women, and scientific research is focusing on the search for particular features of the endometrium that may explain a further predisposition to the onset of endometrial cancer, aimed at improving knowledge of the pathogenetic factors of this disease. The aim of our review is to analyze in detail the results of the literature on the endometrial microbiota in patients with endometrial cancer and to investigate its role. Methods: We performed our research on the Pubmed, Web of Science, and Scopus databases. We searched up to December 2023 and considered manuscripts published from 2000. Only articles in English were included in the search. We excluded studies in which the endometrial microbiota were collected through the vagina or cervical canal. Results: We included in our review a total of five manuscripts at the end of the screening process, and the total number of patients involved was 190. Four studies considered only post-menopausal patients, while one study considered both pre- and post-menopausal patients. In all studies, the microbiota analysis was derived from a post-hysterectomy biopsy. From our review, it emerged that Bacteroidetes, Actinobacteria, Firmicutes, and Proteobacteria are the most represented bacteria in patients with endometrial cancer. These are both Gram-positive and Gram-negative, but predominantly anaerobic bacteria. Conclusions: The reduced microbial diversity and the presence of specific bacteria is often associated with endometrial cancer. Further work on larger population samples, and on healthy women and those affected by endometrial carcinoma, is needed to understand how the endometrial microbiota changes and influences the development of the tumor and whether intervening in the changes in the microbiota will have a therapeutic impact on endometrial carcinoma.
The Impact of the Coronary Artery Calcium Score on the Clinical Outcomes in Patients with Acute Myocardial Infarction
Authors: Sato Hisashi , Sakakura Kenichi , Jinnouchi Hiroyuki , Taniguchi Yousuke , Yamamoto Kei , Tsukui Takunori , Hatori Masashi , Kasahara Taku , Watanabe Yusuke , Ishibashi Shun , Seguchi Masaru , Fujita Hideo
Published: November 2024

Background: It is essential to identify the risk factors for poor clinical outcomes in patients with acute myocardial infarction (AMI). The coronary artery calcium score (CACS) is gathering attention as a predictor for future cardiovascular events. This study aimed to (1) measure CACSs in patients with AMI by non-ECG-gated computed tomography (CT), (2) compare clinical outcomes between patients with a high CACS and a low–intermediate CACS and (3) to elucidate the association between high CACS and clinical outcomes. Methods: We defined the high CACS group as the highest quantile of CACS (Q4) and defined the low–intermediate CACS group as the other quantiles of CACS (Q1–Q3). The primary endpoint was major adverse cardiovascular events (MACE), which were defined as the composite of all-cause death, re-admission for heart failure, non-fatal MI and target vessel revascularization. We included 548 patients with AMI who underwent non-ECG-gated CT and divided them into the high CACS group (CACS ≥ 5346.5, n = 137) and the low–intermediate CACS group (CACS ≤ 5329.3, n = 411). Results: During the median follow-up duration of 535 days, 150 MACE were observed. The Kaplan–Meier curves showed that MACE occurred more frequently in the high CACS group than in the low–intermediate CACS group (p < 0.001). Multivariable Cox hazard analysis revealed that a high CACS was significantly associated with MACE (hazard ratio 1.597, 95% confidence interval 1.081–2.358, p = 0.019) after controlling for multiple confounding factors. Conclusions: Clinical outcomes were worse in AMI patients with a high CACS than in those with a low–intermediate CACS. A high CACS was significantly associated with MACE in multivariate analysis.
Degenerative Cervical Myelopathy: History, Physical Examination, and Diagnosis
Authors: Balmaceno-Criss Mariah , Singh Manjot , Daher Mohammad , Buchbinder Rachelle , Diebo Bassel G. , Daniels Alan H.
Published: November 2024

Background: Degenerative cervical myelopathy is a progressive neurological disorder that is commonly encountered in clinical practice and its incidence is expected to increase alongside the aging population. Given the importance of early and accurate diagnosis in this patient population, this narrative review aims to provide a repository of up-to-date information regarding pertinent patient history, physical exam findings, and potential alternate diagnoses. Methods: The PubMed database was queried for publications from 1 January 2019 to 19 March 2024. The search terms utilized are as follows: cervical myelopathy”, “cervical spondylotic myelopathy”, “degenerative cervical myelopathy”, “epidemiology”, “prevalence”, “incidence”, “etiology”, “diagnosis”, “differential”, “symptoms”, “clinical presentation”, and “atypical symptoms”. The resultant articles were reviewed for relevance and redundancy and are presented within the following categories: Natural History, Epidemiology, Clinical Presentation, Diagnosis, and Management. Results: Myelopathy patients often present with subtle and non-specific symptoms such as sleep disturbances, increased falls, and difficulty driving, which can lead to underdiagnosis and misdiagnosis. Failing to diagnose degenerative cervical myelopathy in a timely manner can result in progressive and irreparable neurological damage. Although many nonoperative treatment modalities are available, surgical decompression is ultimately recommended in most cases to limit further deterioration in neurological function and optimize long-term patient outcomes. Conclusions: A thorough clinical history and physical examination remain the most important diagnostic tools to avoid misdiagnosis and implement early treatment in this patient population.
Optical Attenuation Coefficient-Based En Face Optical Coherence Tomography Imaging for the Reliable Assessment of the Ellipsoid Zone
Authors: Sakai Hiroaki , Kuji Riku , Moriguchi Yoshikiyo , Yamashita Shoko , Takamori Ayako , Tamura Masato , Mino Toshihiro , Akiba Masahiro , Enaida Hiroshi
Published: November 2024

Objective: This study used optical attenuation coefficient (OAC)-based optical coherence tomography (OCT) en face images to assess the ellipsoid zone (EZ) in the foveal region. Methods: This retrospective, observational, cross-sectional study of 41 healthy volunteers and 34 patients with retinal diseases included imaging data acquired using a prototype swept-source OCT system. EZ en face images were generated from OCT raster scan volumes based on OAC, followed by denoising and binarization to quantify the percentage of EZ structural normality or abnormality relative to the total imaging area. We evaluated the reliability of the OAC-based method compared with the OCT signal intensity-based method in healthy and diseased eyes. In addition, the validated program was applied to patients with various retinal conditions. Results: The estimated normal EZ area in healthy eyes was 96.2 ± 5.6% using the OAC-based method versus 89.3 ± 18.8% for the intensity-based method. The OAC-based method effectively mitigated various artifacts caused by retinal blood vessels and other factors in both healthy and diseased eyes. In a pilot study involving six diseased eyes, the area exhibiting EZ structural abnormalities was 27.5–99.6%. Conclusions: The OAC-based EZ assessment robustly suppressed image artifacts and reliably characterized structural abnormalities in the EZ from OCT volumes.
Carbon Nanotube Reinforced Lunar-Based Geopolymer: Curing Conditions
Authors: Prater Janell , Kim Young Hoon
Published: November 2024

Current space exploration focuses on returning to the Moon to expand space exploration capacity by improving technology. The long-term presence of humans and robots on the Moon requires the development of durable habitats for space missions. In recent decades, in situ resource utilization (ISRU) for construction materials has been recognized as a viable option. However, the addition of nanomaterials, which exhibit a high strength-to-weight ratio, has not been incorporated with the ISRU framework in space missions. This paper investigates the impact of carbon nanotubes (CNTs) on lunar simulant-based geopolymers’ compressive strength and water retention. The evaluation of water retention indicates another potential in water recapturing capability. In this study, CNTs can enhance the mechanical properties of lunar simulant-based geopolymer. Two lunar simulants were used, representing the Highland and Mare regions of the Moon. Experimental variables included CNT concentration, four curing regimes (ambient curing, two oven-curing methods, and microwave radiation), and dispersion time in aqueous solutions. Results showed that CNTs can positively influence both strength gain and water retention during curing regimes, but the extent of influence appears to be dependent on simulant type and curing regime. The Highland simulant consistently outperformed the Mare simulant in oven-curing regimes from a strength perspective, regardless of CNT presence. The strength benefits of CNTs were more pronounced at ambient curing temperatures. Even under poor curing conditions—where water availability may be limited at temperatures of 80 °C—CNTs aid in retaining water within the geopolymer matrix, leading to improved strength compared to counterparts. Under the same conditions, a higher concentration of CNTs further confirmed their role in water retention during geopolymerization, with consistently greater water retention observed in samples containing CNTs. Additionally, microwave radiation was explored as an alternative to conventional oven drying, showing potential for reducing curing duration. Finally, the findings suggest that combining CNTs and microwave radiation could enhance water recovery and reuse, contributing to the development of high-strength infrastructure materials on the Moon with reduced energy and cost requirements.
Regulation of Oxygen in the Tumor Microenvironment Synergizes with Immunotherapy to Suppress Tumor Progression
Authors: Wang Shoucheng , Chi Yongjie , Wang Danyang , Zhao Kai , Wang Lianyan
Published: November 2024

Hypoxia represents a crucial characteristic of the tumor microenvironment, which is closely related to cell proliferation, angiogenesis, and metabolic responses. These factors will further promote tumor progression, increase tumor invasion, and enhance tumor metastasis potential. A hypoxic microenvironment will also inhibit the activity of infiltrated immune cells in the tumor microenvironment, leading to the failure of cancer immunotherapy. Additionally, the hypoxic tumor microenvironment contributes to resistance to conventional therapies and leads to unfavorable prognoses. This review discusses advancements in strategies aimed at ameliorating tumor hypoxia within the microenvironment and modulating immune cell responses against tumors.
Enhanced YOLOv8 Ship Detection Empower Unmanned Surface Vehicles for Advanced Maritime Surveillance
Authors: Haijoub Abdelilah , Hatim Anas , Guerrero-Gonzalez Antonio , Arioua Mounir , Chougdali Khalid
Published: November 2024

The evolution of maritime surveillance is significantly marked by the incorporation of Artificial Intelligence and machine learning into Unmanned Surface Vehicles (USVs). This paper presents an AI approach for detecting and tracking unmanned surface vehicles, specifically leveraging an enhanced version of YOLOv8, fine-tuned for maritime surveillance needs. Deployed on the NVIDIA Jetson TX2 platform, the system features an innovative architecture and perception module optimized for real-time operations and energy efficiency. Demonstrating superior detection accuracy with a mean Average Precision (mAP) of 0.99 and achieving an operational speed of 17.99 FPS, all while maintaining energy consumption at just 5.61 joules. The remarkable balance between accuracy, processing speed, and energy efficiency underscores the potential of this system to significantly advance maritime safety, security, and environmental monitoring.
Dynamic Response Assessment of Floating Offshore Wind Turbine Mooring Systems with Different In-Line Tensioner Configurations Based on Fully Coupled Load Calculations
Authors: Li Wenhua , Du Guanlin , Lin Shanying , Chuang Zhenju , Wang Fei
Published: November 2024

In-line tensioning technology has significantly reduced the cost barriers that previously hindered the expansion of the floating offshore wind industry. However, assessing the impact of in-line tensioners on the dynamic response of floating offshore wind turbines (FOWTs) lacks effectiveness, and the relevant mooring configuration specifications are not complete. Thus, a fully coupled calculation method is introduced in this paper to solve the relevant issues in mooring systems with in-line tensioners using a classic spar platform model. Three distinct design scenarios were selected to study the variation in mooring configurations of in-line tensioners along different mooring lines and at varied positions within each line. The potential occurrence of reverse tension phenomena was deliberated and assessed. We identified the varying tension patterns at the fairlead and in-line tensioner locations in mooring systems with in-line tensioners, and the influence of such variations on platform dynamics. The findings also demonstrate that the appropriate configuration of in-line tensioners should be selected to avoid the risk of reverse tension. This research has potential to contribute to the security and economy of the deployment of this emerging in-line mooring method.
An Assessment of the Tipping Point Behavior for Shoreline Retreat: A PCR Model Application at Vung Tau Beach, Vietnam
Authors: Wang Xiaoting , Dastgheib Ali , Reyns Johan , Li Fan , Duong Trang Minh , Zhang Weiguo , Sun Qinke , Ranasinghe Roshanka
Published: November 2024

Storm waves and rising sea levels pose significant threats to low-lying coastal areas, particularly sandy beaches, which are especially vulnerable. The research on the long-time-scale changes in sandy coasts, especially the identification of tipping points in the shoreline-retreat rate, is limited. Vung Tau beach, characterized by its low terrain and rapid tourism-driven economic growth, was selected as a typical study area to quantify the shoreline retreat throughout the 21st century under various sea-level rise (SLR) scenarios, and to identify the existence of tipping points by investigating the projected annual change in shoreline retreat (m/yr). This study employs the Probabilistic Coastline Recession (PCR) model, a physics-based tool specifically designed for long-term coastline change assessments. The results indicate that shoreline retreat accelerates over time, particularly after a tipping point is reached around 2050 in the SSP1-2.6, SSP2-4.5, and SSP5-8.5 scenarios. Under the SSP5-8.5 scenario, the median retreat distance is projected to increase from 19 m in 2050 to 89 m by 2100, nearly a fourfold rise. In comparison, the retreat distances are smaller under the SSP1-2.6 and SSP2-4.5 scenarios, but the same accelerating trend is observed beyond 2050. These findings highlight the growing risks associated with sea-level rise, especially the rapid increase in exceedance probabilities for retreat distances by the end of the century. By 2100, the probability of losing the entire beach at Vung Tau is projected to be 22% under SSP5-8.5. The approach of identifying tipping points based on the PCR model presented here can be applied to other sandy coastal regions, providing critical references for timely planning and the implementation of adaptation measures.
Extent of Benthic Habitat Disturbance by Offshore Infrastructure
Authors: Cerrato Robert M. , Flood Roger D. , Bopp Justin , Bokuniewicz Henry J.
Published: November 2024

The effects of the interaction between sandy, mobile, low-relief (sorted) bedforms and two sewage outfalls were investigated along the south shore of Long Island, NY. Sand bedforms at scales from ripples to ridges are common on continental shelves. In dynamic environments, these features can migrate 10s to 100s of meters per year, especially during storms. Beyond engineering considerations, little is known of the interaction between these mobile features and anthropogenic structures. Modification of bedform topography and sediment grain-size distribution can be expected to alter the species composition, abundance, and diversity of the benthic community. At the study site, the interaction increased the scour of modern fine- to medium-grained sediments extending out to a kilometer and uncovered coarser-grained late Pleistocene sediments. This alteration of the seafloor in turn resulted in changes in composition, higher abundance, and lower diversity in the species assemblage found in the impacted area. The most advantaged species was Pseudunciola obliquua, a sightless, tube-building, surface deposit-feeding amphipod that is known to prefer a dynamic coarse sand habitat. Overall, the ecological effects of artificial structures on a wave-dominated seabed with sorted bedforms have not been adequately assessed. In particular, and of great importance, is the pending large-scale development of wind farms off the East Coast of the U.S.
Assessment of Vessel Mooring Conditions Using Satellite Navigation System Real-Time Kinematic Application
Authors: Filina-Dawidowicz Ludmiła , Paulauskas Vytautas , Paulauskas Donatas , Senčila Viktoras
Published: November 2024

When mooring a ship near the quay, it is important to monitor its speed at the time of contact with the quay to ensure the safe execution of the mooring operation. During mooring, the speed of the ship must not exceed specified values; therefore, it is very important to have the possibility to measure it with high accuracy and its appropriate adjustment. This article aims to present the assessment methodology of the forces acting on quay equipment when a ship is mooring using data provided by the real-time kinematic (RTK) application of the navigation satellite system, as well as a way to calculate the comparative index, which can show the advantages of using data provided by high-accuracy measurement systems compared with the typical one. The methodology of assessing the forces acting on quay equipment when the ship is mooring using data provided by high-precision systems was applied. To verify the developed methodology, the experiments were carried out on real ships and using a calibrated simulator. Based on the research results, it was stated that when planning and managing ships’ mooring operations in ports using data provided by the RTK application, it is possible to reduce the planned energy absorption of quay fenders up to 1.5–1.8 times while preparing the investment in quay development. The implementation of the developed methodology may contribute to the improvement of navigation safety when ships are mooring near the quays and thus allow for the reduction in the probability of undesirable situations occurring. The research results may be of interest to representatives of seaports authorities, traffic management offices, shipowners and other institutions involved in safe ships’ navigation in seaports and approaches to them.
An Innovative Online Adaptive High-Efficiency Controller for Micro Gas Turbine: Design and Simulation Validation
Authors: Yang Rui , Liu Yongbao , He Xing , Liu Zhimeng
Published: November 2024

In this article, an innovative online adaptive high-efficiency control strategy is proposed to improve the power generation efficiency of a marine micro gas turbine under partial load. Firstly, a mathematical model of the micro-gas turbine is established, and a control strategy consisting of an on-board prediction model and an online update model is proposed. To evaluate the performance changes of the gas turbine, we applied deep learning techniques to enhance the extreme learning machine (ELM) algorithm, resulting in the development of a high-precision, high-real-time deep extreme learning machine (DL_ELM) prediction model. This model effectively monitors changes in the gas turbine’s performance. Furthermore, an online time-series deep extreme learning machine with a dynamic forgetting factor (DFF_DL_OSELM) model is designed to achieve the real-time tracking of performance variations. When the DL_ELM model detects a gas turbine’s performance change, a particle swarm optimization (PSO) algorithm is employed to iteratively calculate the DFF_DL_OSELM model, determining the optimal speed control scheme to ensure the gas turbine operates at maximum efficiency. To validate the superiority of the proposed control strategy, a comparison is made with traditional high-efficiency control strategies based on polynomial fitting and BP neural networks. The results demonstrate that although all three strategies can achieve efficient operation under constant conditions, traditional strategies fail to identify and adjust to performance changes in real time, leading to decreased control performance and potential engine damage as engine characteristics degrade. In contrast, the proposed online adaptive control strategy dynamically adjusts the speed control plan based on performance degradation, ensuring that the gas turbine operates efficiently while keeping the turbine inlet and exhaust temperatures within safe limits.
Centrifugal Test Study on the Vertical Uplift Capacity of Single-Cylinder Foundation in High-Sensitivity Marine Soil
Authors: Wei Mingzhe , Ye Yanghui , Zhao Wei , Wang Zehao , Ge Fuhao , Nian Tingkai
Published: November 2024

Offshore wind power is a new type of clean energy with broad development prospects. Accurate analysis of the uplift capacity of offshore wind turbine foundations is a crucial prerequisite for ensuring the safe operation of wind turbines under complex hydrodynamic conditions. However, current research on the uplift capacity of suction caissons often neglects the high-sensitivity characteristics of marine soils. Therefore, this paper first employs the freeze–thaw cycling procedure to prepare high-sensitivity saturated clay. Subsequently, a single−tube foundation for wind turbines is constructed within a centrifuge through a penetration approach. Ten sets of centrifuge model tests with vertical cyclic pullout are conducted. Through comparative analysis, this study explores the pullout capacity and its variation patterns of suction caisson foundations in clay with different sensitivities under cyclic loading. This research indicates the following: (1) The preparation of high-sensitivity soil through the freeze−thaw procedure is reliable; (2) the uplift capacity of suction caissons in high−sensitivity soil rapidly decreases with increasing numbers of cyclic loads and then tends to stabilize. The cumulative displacement rate of suction caissons in high-sensitivity soil is fast, and the total number of pressure–pullout cycles required to reach non-cumulative displacement is significantly smaller than that in low-sensitivity soil; (3) the vertical cyclic loading times and stiffness evolution patterns of single-tube foundations, considering the influence of sensitivity, have been analyzed. It was found that the secant stiffness exhibits a logarithmic function relationship with both the number of cycles and sensitivity. The findings of this study provide assistance and support for the design of suction caissons in high-sensitivity soils.
Nonlinear Dynamic Stability Analysis of Ground Effect Vehicles in Waves Using Poincaré–Lindstedt Perturbation Method
Authors: Masri Jafar , Dala Laurent , Huard Benoit
Published: November 2024

In this study, we present an analytical tool that can be used to predict the nonlinear dynamic response of ground effect vehicles (GEVs) advancing through sinusoidal head-sea waves. GEVs exhibit a unique instability phenomenon known as porpoising, which is an oscillatory motion along the heave and pitch axes that can cause serious structural damage. The heaving and pitching equations of motion are presented in the form of coupled, forced, and nonlinear Duffing-type equations with cubic nonlinearity. The analytical model developed in this study leverages the Poincaré–Lindstedt perturbation method to express the amplitude and frequency of motion in terms of all physical parameters. The accuracy and reliability of the proposed model were validated through computational fluid dynamics (CFD) simulations based on incompressible unsteady Reynolds-averaged Navier–Stokes (RANS) equations. The results show a strong agreement between the analytical tool and the CFD simulations, with minor discrepancies due to assumptions inherent in the 2D simulations, particularly the assumption that seawater only passes beneath the hull, resulting in increased buoyancy forces and reduced damping. This study offers a novel and practical method for predicting the dynamic stability of GEVs under realistic sea conditions, potentially enhancing safety and operational efficiency by mitigating the risks associated with porpoising.
Encapsulation, Shelf Life, and Virulence of Batkoa sp. Against Dalbulus maidis
Authors: Silva Daniela Milanez , Iwanicki Natasha Sant’ Anna , Muskat Linda Claire , Patel Anant V. , Delalibera Júnior Italo
Published: November 2024

Batkoa is a genus of entomophthoralean fungi often associated with insect epizootics, particularly in phytophagous hemipterans. Encapsulation has become a promising strategy for improving the shelf life and sporulation of these fungi post-application. This study aims to (i) compare the virulence of the submerged propagules and primary conidia of Batkoa sp. ESALQ1199 against Dalbulus maidis; (ii) formulate submerged propagules in calcium alginate beads with co-formulants; (iii) assess the colony-forming units and sporulation of encapsulated beads dried with different kaolin concentrations (0%, 2%, 4%, 8% and 10%); (iv) determine the shelf life of dried bead formulations containing 10% kaolin, comparing washed and unwashed beads treated with a 4% sucrose solution; and (v) assess the sporulation capacity of beads with 10% kaolin, washed and unwashed with 4% sucrose solution, over time under humid conditions. Our results demonstrated that primary conidia and submerged propagules effectively killed 82.4% and 57.8% of adult corn leafhoppers, respectively. Co-formulants maintained viability above 80% in dried propagules, while control samples dropped to 45%, indicating the sensitivity of submerged propagules to the drying process. Encapsulated Batkoa sp. retained the same concentration of viable propagules per bead and the number of conidia produced (sporulation) for 30 days at 28 °C. The sporulation of fresh beads increased during the incubation period, plateauing after 27 days. This suggests that Batkoa sp. beads can produce primary conidia under humid field conditions, serving as a potential inoculum source for new infections.
Evaluation of Fifteen 5,6-Dihydrotetrazolo[1,5-c]quinazolines Against Nakaseomyces glabrata: Integrating In Vitro Studies, Molecular Docking, QSAR, and In Silico Toxicity Assessments
Authors: Antypenko Lyudmyla , Antypenko Oleksii , Fominichenko Alina , Karnaukh Iryna , Kovalenko Serhii , Arisawa Mieko
Published: November 2024

Nakaseomyces glabrata (Candida glabrata), the second most prevalent Candida pathogen globally, has emerged as a major clinical threat due to its ability to develop high-level azole resistance. In this study, two new 5,6-dihydrotetrazolo[1,5-c]quinazoline derivatives (c11 and c12) were synthesized and characterized using IR, LC-MS, 1H, and 13C NMR spectra. Along with 13 previously reported analogues, these compounds underwent in vitro antifungal testing against clinical N. glabrata isolates using a serial dilution method (0.125–64 mg/L). Remarkably, compounds c5 and c1 exhibited potent antifungal activity, with minimum inhibitory concentrations of 0.37 μM and 0.47 μM, respectively—about a 20-fold improvement in μM concentration over standard drugs like amphotericin B, caspofungin, and micafungin. A detailed structure–activity relationship analysis revealed crucial molecular features enhancing antifungal potency. Extensive molecular docking studies across 18 protein targets explored potential binding pockets and affinities of the lead compounds. A robust 3D-QSAR model, incorporating molecular descriptors Mor26m and Mor29e, displayed good predictive ability for antifungal activity. In silico predictions indicated an absence of herbicidal effect, negligible environmental toxicity (to honeybees, avian species, and aquatic organisms), and mild human toxicity concerns for these compounds. This comprehensive approach aims to develop novel and effective antifungal compounds against the clinically relevant pathogen N. glabrata.
Pseudophylloporus Gen. nov. and Rubroleccinum Gen. nov., Two New Genera Revealed by Morphological and Phylogenetic Evidences in the Family Boletaceae from Subtropical China
Authors: Qin Hua-Zhi , Wang Yi , Lin Wen-Fei , Zeng Hui , Hu Li-Gui , Ke Bin-Rong , Zeng Zhi-Heng , Liang Zhi-Qun , Zeng Nian-Kai
Published: November 2024

Boletaceae, the largest and most diverse family of Boletales (Agaricomycetes and Basidiomycota), is both ecologically and economically important. Although many taxa have been described in China, the diversity of the family still remains incompletely understood. In the present study, Pseudophylloporus baishanzuensis gen. nov., sp. nov. and Rubroleccinum latisporus gen. nov., sp. nov. are proposed based on morphological and molecular phylogenetic analyses. These findings contribute to a deeper understanding of the diversity within the Boletaceae family.
Role of UeMsb2 in Filamentous Growth and Pathogenicity of Ustilago esculenta
Authors: Jiang Wanlong , Hu Yingli , Wu Juncheng , Hu Jianglong , Tang Jintian , Wang Ran , Ye Zihong , Zhang Yafen
Published: November 2024

Ustilago esculenta is a dimorphic fungus that specifically infects Zizania latifolia, causing stem swelling and the formation of an edible fleshy stem known as jiaobai. The pathogenicity of U. esculenta is closely associated with the development of jiaobai and phenotypic differentiation. Msb2 acts as a key upstream sensor in the MAPK (mitogen-activated protein kinase) signaling pathway, playing critical roles in fungal hyphal growth, osmotic regulation, maintenance of cell wall integrity, temperature adaptation, and pathogenicity. In this study, we cloned the UeMsb2 gene from U. esculenta (GenBank No. MW768949). The open reading frame of UeMsb2 is 3015 bp in length, lacks introns, encodes a 1004-amino-acid protein with a conserved serine-rich domain, and is localized to the vacuole. Expression analysis revealed that UeMsb2 is inducibly expressed during both hyphal growth and infection processes. Deletion of UeMsb2 did not affect haploid morphology or growth rate in vitro but significantly impaired the strain’s mating ability, suppressed filamentous growth, slowed host infection progression, and downregulated the expression of b signaling pathway genes associated with pathogenicity. Notably, the deletion of UeMsb2 did not influence the in vitro growth of U. esculenta under hyperosmotic, thermal, or oxidative stress conditions. These findings underscore the critical role of UeMsb2 in regulating the pathogenicity of U. esculenta. This study provides insights into the interaction between U. esculenta and Z. latifolia, particularly the mechanisms that drive host stem swelling.
Multifocal Electroretinography Changes in Patients with Late-Stage Age-Related Macular Degeneration (AMD) After Smaller-Incision New-Generation Implantable Miniature Telescope (SING IMT): A Case Series
Authors: Landini Luca , Boscia Giacomo , Vidal-Aroca Faustino , Niro Alfredo , Pastore Valentina , Piepoli Marina , Viggiano Pasquale , Grassi Maria Oliva , Giancipoli Ermete , Pignataro Maria Grazia , Alessio Giovanni , Levy Marc H. , Sborgia Giancarlo , Boscia Francesco
Published: November 2024

The smaller-incision new-generation implantable miniature telescope (SING IMT) represents an advancement over the previous model, WA-IMT, serving as a unilateral prosthetic device for patients with late-stage age-related macular degeneration (AMD). Purpose: This study aims to report changes in multifocal electroretinography (mfERG) 6 months post–SING IMT implantation. Methods: In this case series, we prospectively evaluated a cohort of phakic patients with late-stage AMD who underwent SING IMT implantation at the Ophthalmology Unit, University of Bari Aldo Moro, Italy. We assessed best-corrected distance visual acuity (BCDVA) and best-corrected near visual acuity (BCNVA) preoperatively and at 6 months postoperatively. Additionally, mfERGs were conducted using Retimax (CSO, Florence, Italy). Results: All four treated patients showed an increase in both BCDVA and BCNVA at the 6-month follow-up. Additionally, all eyes demonstrated increased P1 density at this time point, with the greatest augmentation observed at the central fixation point, gradually diminishing across the five concentric rings. While all patients displayed a general increase in P1 amplitude, the third patient exhibited a slight decrease in the foveal region. Conclusions: In this case series with four cases, the new generation implantable miniature telescope, SING IMT, demonstrates promising results in enhancing mfERG parameters in patients with late-stage AMD. Six months post-surgery, we observed an augmentation in both P1 density and amplitude, predominantly at the fixation point and gradually tapering in the surrounding concentric rings.
Using Public Participation Geographic Information System to Study Social Cohesion and Its Relationship with Activities and Specific Landscape Characteristics in Shanghai’s Modern Historic Parks
Authors: Chen Yuxian , Tang Jiajia , Dai Daixin
Published: November 2024

Evaluating the perceptions of social cohesion provides an elaborated understanding of the commemorative values of modern heritage. However, little attention has been paid to China’s modern historic parks (MHPs) due to the difficulty of capturing spatially explicit information. Utilizing a public participation GIS, we quantified four types of social cohesion in Shanghai’s existing MHPs, and analyzed their relationships with different types of activities and specific landscape features. The results show that the MHPs are rich in social cohesion, especially in belonging and place attachment. Social cohesion is spatially associated with leisure and sightseeing activities. The perception of different types of social cohesion varies across spaces with different landscape features. Our findings validate the composition of commemorative values of MHPs, and offer a reference for balancing the preservation and regeneration of modern heritage.
Simulating Land Use Through Integrated Soil and Water Ecosystem Services: Case Study of Yanguan City in China
Authors: Mu Hongxu , Zhang Xueli , Jian Shengqi
Published: November 2024

Rapid urbanization and climate change are driving significant shifts in land use patterns, which pose challenges for maintaining ecosystem services such as water supply and soil retention. This study explores the impact of land use structures on water supply, soil retention, and carbon sequestration by simulating multiple land use scenarios forecasted for the year 2050 in Yangquan City. Using multi-objective planning methods and the patch-generating land use simulation (PLUS) model, the research examines changes in land use, including an increase in urban construction land and a reduction in cultivated land, addressing future demands for population accommodation and food production. The findings reveal a hierarchical land distribution centered around urban areas, with cultivated land, grassland, and forest land in succession. As greenhouse gas emissions rise, water yield and soil retention also increase. Under different climate conditions, Yangquan City’s water yield and soil retention demonstrate significant variation, with higher emissions linked to more substantial increases in both water yield and soil retention. These results highlight the dynamic interactions between land use changes and ecosystem services in response to climate change.
Pluractional Motion Verbs in Turkish
Authors: Key Greg , Ótott-Kovács Eszter
Published: November 2024

In this paper, we examine a small set of motion verbs in Turkish bearing the so-called ‘reciprocal’ suffix -(I)ş: kaç-ış- ‘flee in all directions’ (cf. kaç- ‘flee’), koş-uş- ‘run helter-skelter’ (cf. koş- ‘run’), uç-uş- ‘fly helter-skelter’ (cf. uç- ‘fly’). It has previously been claimed that these are collective or sociative verbs entailing a low elaboration of events and/or plural participants. We show that these -(I)ş-marked verbs, in fact, require a higher degree of individuation of events than do their unmarked counterparts. Furthermore, we show that a stipulation directly associating the suffix with a requirement for a plural subject is both unnecessary and inadequate. Instead, we propose that the pluractional under investigation manipulates the denoted events’ spatial and temporal properties in such a way that the predicate can only be felicitously used if it combines with a plural external argument.
AI Accountability in Judicial Proceedings: An Actor–Network Approach
Authors: Contini Francesco , Ontanu Elena Alina , Velicogna Marco
Published: November 2024

This paper analyzes the impact of AI systems in the judicial domain, adopting an actor–network theory (ANT) framework and focusing on accountability issues emerging when such technologies are introduced. Considering three different types of AI applications used by judges, this paper explores how introducing non-accountable artifacts into justice systems influences the actor–network configuration and the distribution of accountability between humans and technology. The analysis discusses the actor–network reconfiguration emerging when speech-to-text, legal analytics, and predictive justice technologies are introduced in pre-existing settings and maps out the changes in agency and accountability between judges and AI applications. The EU legal framework and the EU AI Act provide the juridical framework against which the findings are assessed to check the fit of new technological systems with justice system requirements. The findings show the paradox that non-accountable AI can be used without endangering fundamental judicial values when judges can control the system’s outputs, evaluating its correspondence with the inputs. When this requirement is not met, the remedies provided by the EU AI Act fall short in costs or in organizational and technical complexity. The judge becomes the unique subject accountable for the use and outcome of a non-accountable system. This paper suggests that this occurs regardless of whether the technology is AI-based or not. The concrete risks emerging from these findings are that these technological innovations can lead to undue influence on judicial decision making and endanger the fair trial principle.
Spirulina (Arthrospira platensis) Used as Functional Feed Supplement or Alternative Protein Source: A Review of the Effects of Different Dietary Inclusion Levels on Production Performance, Health Status, and Meat Quality of Broiler Chickens
Authors: Lestingi Antonia , Alagawany Mahmoud , Di Cerbo Alessandro , Crescenzo Giuseppe , Zizzadoro Claudia
Published: November 2024

The broiler industry is pivotal in meeting the growing global demand for highly nutritious animal protein foods. Hence, there is a continuous interest in identifying novel, alternative, and even unconventional feed resources that could help sustainably support chicken meat production and quality. In this view, the microalga Spirulina (Arthrospira, formerly Spirulina, platensis), due to its unique chemical composition and some ecological advantages offered by its cultivation over traditional agriculture, has attracted great attention in the poultry sector for potential application in broiler diets, either as a functional supplement or a replacer of conventional protein sources such as soybean meal. The studies conducted so far seem to have confirmed many of the initial expectations regarding the advantages that may derive from dietary Spirulina supplementation, documenting its capacity to positively influence the intestinal and general health status of broiler chickens, leading to improved or preserved productive performance (under normal or challenging conditions, respectively), as well as to increased disease resistance and survivability. Furthermore, dietary Spirulina supplementation has been shown to induce positive changes in some important traits of broiler meat quality. However, at present, the inclusion of Spirulina in broiler diet, especially but not solely in relation to the use as an alternative protein source, presents several technical and economic limitations. To increase the overall awareness around the actual usefulness and practical usability of Spirulina as a novel natural component of the broiler diet, this review paper seeks to provide a comprehensive and integrated presentation of what is currently known about this topic, highlighting critical issues that are still pending and would require further research efforts.
In Vitro Anticancer Effects of Aqueous Leaf Extract from Nepeta nuda L. ssp. nuda
Authors: Gospodinova Zlatina , Antov Georgi , Stoichev Svetozar , Zhiponova Miroslava
Published: November 2024

Despite significant efforts, cancer remains the second leading cause of mortality worldwide. The medicinal plant Nepeta nuda L. represents a valuable source of biologically active compounds with pharmacological activities including antioxidant, anti-inflammatory, antimicrobial, and antiviral. This study aimed to assess the antiproliferative potential and mechanisms of action of aqueous extract from the leaves of wild-grown N. nuda. Cancer cell lines, MDA-MB-231, MCF7 (breast), HT29, Colon 26 (colon), and HepG2 (liver cancer), and a non-cancerous skin cell line, BJ, were assessed for antiproliferative activity by MTT assay and observation of cell morphological alterations. The cancer cell line that was most sensitive to the extract was further studied for apoptotic alterations by Annexin V/propidium iodide staining, colony-forming assay, and qRT-PCR analysis. The results revealed that the plant extract inhibited the proliferation of all investigated cancer cell lines with the strongest cytostatic effect on Colon 26 cells with a half maximal inhibitory concentration (IC50) value of 380.2 μg/mL and a selectivity index (SI) of 3.5. The extract significantly inhibited the ability of cells to form colonies, exhibited considerable proapoptotic potential involving the participation of the CASP8 gene, and increased the expression levels of ATG3 and the BECN1 gene, which suggests a role of autophagic cell death in the antitumor action.
Correction: Li et al. Preliminary Evaluation of Protective Efficacy of Inactivated Senecavirus A on Pigs. Life 2021, 11, 157
Authors: Li Yuwan , Zhang Yangyi , Liao Yingxin , Sun Yawei , Ruan Yang , Liu Chenchen , Zhang Mengru , Li Fangfang , Li Xiaowen , Fan Shuangqi , Yi Lin , Ding Hongxing , Zhao Mingqiu , Fan Jindai , Chen Jinding
Published: November 2024

In the original publication, there was a mistake in Figure 1B(b,c) as published [...]
Antibiotic Resistance Genes Detection in Several Local Cyanobacteria Isolates
Authors: Buniya Harith K. , Mohammed Nuha A. , Al-Hayani Dhyauldeen Aftan
Published: November 2024

Antibiotic resistance in cyanobacteria represents a global threat to public health. The widespread presence of cyanobacteria in aquatic environments exposes them to antibiotic contamination. Cyanobacteria are also in direct contact with pathogenic bacteria containing antibiotic-resistance genes (ARGs), which impart these characteristics to them. This study aims to examine the presence of some ARGs in locally isolated cyanobacteria species, Spirulina laxa, Chroococcus minutes, Oscillatoria princeps, Oscillatoria proteus, Oscillatoria terebriformis, and Lyngbya epiphytica, and compare the presence of these genes in two pathogenic bacteria, Escherichia coli and Klebsiella pneumoniae. Ampicillin (Ap) and erythromycin (Em) resistance genes were detected in five algal samples. Meanwhile, Chloramphenicol (Cm) and gentamicin (Gm) resistance genes were apparent in only two species. Genes encoding resistance towards kanamycin (Km) and spectinomycin (Sp) were recorded in three specimens. It was also found that E. coli possessed resistance genes for four antibiotics, ampicillin (Ap), erythromycin (Em), gentamicin (Gm), and kanamycin (Km), whereas K. pneumoniae was resistant towards three antibiotics, ampicillin (Ap), gentamicin (Gm), and kanamycin (Km). The results show that there is a match in antibiotic-resistance genes in both cyanobacteria and pathogenic bacteria. Suggesting the possibility that cyanobacteria could acquire ARGs from the environment through horizontal gene transfer. Thus, freshwater cyanobacteria may play a significant role in the prevalence of ARGs in their environment.
Analysis of Microstructural and Wear Mechanisms for 3D-Printed PET CF15 Using Box–Behnken Design
Authors: Portoaca Alexandra Ileana , Dinita Alin , Ripeanu Razvan George , Tănase Maria
Published: November 2024

We examined the impact of 3D-printing parameters, such as the deposition pattern, deposition speed, and layer height, on the tribological performance measured through the coefficient of friction and cumulative linear wear. Optimizing these factors can significantly influence material wear and friction, which is critical for ensuring durability and functionality in practical applications like a cylindrical gear assembly for a vertical-articulated robot. The purpose of the study was to investigate these relationships by employing the Box–Behnken design (BBD) method to systematically analyze the effects of these parameters, while also using scanning electron microscopy (SEM) for detailed microstructural characterization. The findings aim to provide insights that can guide the development of more efficient and wear-resistant 3D-printed materials. The strong impact of layer height on CLW was noted, showing that lower layer heights can either improve or worsen wear depending on the combination of speed and pattern, with layer height playing a dominant role in determining wear performance. Lower speeds and specific patterns, particularly lines and concentric patterns, tend to result in higher COF values. The validation test results, with a COF of 0.2215 and CLW of 29.2075, closely align with the predicted values of 0.2064 and 27.3, showing small percentage errors of 7.3% for COF and 6.5% for CLW.