bluuebunny/crossref_metadata_2025 huggingface dataset:
Assessment of Acid Phosphatase Production by In vitro Cultures of Atropa acuminata
Authors: Khan Saima , Katoch Meenu , Mallubhotla Sharada , Gupta Suphla , Sambyal Manju , Ahuja Ashok
Published: September 2016

The potential of various culture lines of Atropa acuminata were investigated for resourcing acid phosphatase (ACP) (3.1.3.2). Crude enzyme extract comprised of a mixture of four isoforms, distinguishable by polyacrylamide gel electrophoresis (PAGE) with molecular weight ranging from 39 to 215 kDa. In vitro regenerated proliferative shoots, callus and roots showed higher specific activity (2.49, 3.41, 2.91 U/mg protein, respectively) as compared to in vivo grown plants (0.71 U/mg protein). ACP activity in root cultures increased progressively up to 4.6 U/mg during the entire growth period (2 ? 24 weeks), whereas in case of shoot cultures, the specific activity escalated to 2.49 U/mg at 8 weeks, which then declined subsequently (1.95 U/mg). Similarly, callus cultures initially showed a higher phosphohydrolytic activity (3.41 U/mg protein) until 8 weeks by which period, it decreased with the passage of growth period. The present studies reveal an alternate system for resourcing of ACP from Atropa acuminata.Plant Tissue Cult. & Biotech. 26(1): 15-23, 2016 (June)
Somatic Embryogenesis and Genetic Uniformity of Cassava Plants Regenerated from Secondary Somatic Cotyledons Preserved in Osmotic Agents
Authors: Opabode Jelili T , Ajibola Olufemi V , Oyelakin Olufemi O , Akinyemiju Oluyemisi A
Published: September 2016

Somatic embryogenesis, plant regeneration and genetic stability of regenerants grown from cassava secondary somatic cotyledon preserved at 160C on medium containing mannitol or sorbitol alone and their combinations were investigated. Irrespective of osmotic agents in the medium, survival of cotyledon explant, frequency of somatic embryos, shoot induction, number of somatic embryo per explant, shoot elongation and rooting decreased as preservation period increased. The highest survival rate of cotyledon explants, frequency of somatic embryos, shoot induction and shoot elongation were observed on media containing 2% mannitol. However, the highest per cent rooting occurred on medium containing mannitol alone at 8 months after storage (MAS) and on media containing mannitol or sorbitol alone at 16 MAS. RAPD analysis suggested genetic uniformity among regenerants and their control plant. Osmotic preservation of secondary somatic embryos of cassava on 2% mannitol at 160C is the best slow?growth method.Plant Tissue Cult. & Biotech. 26(1): 47-54, 2016 (June)
Influence of Plant Growth Regulators and Medium Strength on Micropropagation of the Biodiesel Plant, Jatropha curcus
Authors: Rady Mohamed R , Sayed El , Mervat M , El Sayed Mohamed , Aly Usama I
Published: September 2016

Leaf explants were cultured to evaluate the effect of different auxins and cytokinins and its concentrations; MS salts on micropropagation of the promising biodiesel Jatropha curcas plant under Egyptian conditions. Results showed that shoot initiated on 0.5 mg/l BA + 0.25 mg/l IBA. Multiplication and elongation were found to be the best using 0.5 mg/l BA in combination with 0.05 mg/l IBA. The multiple shoots were cultured on MS or half strength of MS supplemented with different concentrations of IAA and IBA for rooting phase. Half strength of MS containing 1.0 mg/l IAA was the best for rooting of micropropagated shoots. The rooted plantlets were acclimated in sand : peat?moss mixture (1 : 1) successfully.Plant Tissue Cult. & Biotech. 26(1): 85-96, 2016 (June)
SeqDev: An Algorithm for Constructing Genetic Elements Using Comparative Assembly
Authors: Rahman Tasnim , Heickal Hasnain , Tabrejee Shamira , Chowdhury Md Miraj Kobad , Sarwar Sheikh Muhammad , Shoyaib Mohammad
Published: September 2016

With the availability of recent next generation sequencing technologies and their low cost, genomes of different organisms are being sequenced frequently. Therefore, quick assembly of genome, transcriptome, and target contigs from the raw data generated through the sequencing technologies has become necessary for better understanding of different biological systems. This article proposes an algorithm, namely SeqDev (Sequence Developer) for constructing contigs from raw reads using reference sequences. For this, we considered a weighted frequency?based consensus mechanism named BlastAssemb for primary construction of a sequence with gaps. Then, we adopted suffix array and proposed a gap filling search (GFS) algorithm for searching the missing sequences in the primary construct. For evaluating our algorithm, we have chosen Pokkali (rice) raw genome and Japonica (rice) as our reference data. Experimental results demonstrated that our proposed algorithm accurately constructs promoter sequences of Pokkali from its raw genome data. These constructed promoter sequences were 93 ? 100% identical with the reference and also aligned with 96 ? 100% of corresponding reference sequences with eValue ranging from 0.0 ? 2e-14. All these results indicated that our proposed method could be a potential algorithm to construct target contigs from raw sequences with the help of reference sequences. Further wet lab validation with specific Pokkali promoter sequence will boost this method as a robust algorithm for target contig assembly.Plant Tissue Cult. & Biotech. 26(1): 105-121, 2016 (June)
Morphology of Donor and Recipient Nerves Utilised in Nerve Transfers to Restore Upper Limb Function in Cervical Spinal Cord Injury
Authors: Messina Aurora , Van Zyl Natasha , Weymouth Michael , Flood Stephen , Nunn Andrew , Cooper Catherine , Hahn Jodie , Galea Mary
Published: September 2016

Loss of hand function after cervical spinal cord injury (SCI) impacts heavily on independence. Multiple nerve transfer surgery has been applied successfully after cervical SCI to restore critical arm and hand functions, and the outcome depends on nerve integrity. Nerve integrity is assessed indirectly using muscle strength testing and intramuscular electromyography, but these measures cannot show the manifestation that SCI has on the peripheral nerves. We directly assessed the morphology of nerves biopsied at the time of surgery, from three patients within 18 months post injury. Our objective was to document their morphologic features. Donor nerves included teres minor, posterior axillary, brachialis, extensor carpi radialis brevis and supinator. Recipient nerves included triceps, posterior interosseus (PIN) and anterior interosseus nerves (AIN). They were fixed in glutaraldehyde, processed and embedded in Araldite Epon for light microscopy. Eighty percent of nerves showed abnormalities. Most common were myelin thickening and folding, demyelination, inflammation and a reduction of large myelinated axon density. Others were a thickened perineurium, oedematous endoneurium and Renaut bodies. Significantly, very thinly myelinated axons and groups of unmyelinated axons were observed indicating regenerative efforts. Abnormalities exist in both donor and recipient nerves and they differ in appearance and aetiology. The abnormalities observed may be preventable or reversible.
Long-Term Engagement in Formal Volunteering and Well-Being: An Exploratory Indian Study
Authors: Elias Jereesh , Sudhir Paulomi , Mehrotra Seema
Published: September 2016

Sustained engagement in volunteering and its correlates have been examined in many studies across the globe. However, there is a dearth of research that explores the perspectives of long-term formal volunteers on the nature of changes perceived in oneself as a result of volunteering. Moreover, the linkages between psychological well-being and volunteering have been insufficiently explored. The present study was aimed at addressing these gaps. A heterogeneous sample of 20 long-term formal volunteer engaged in volunteering across different voluntary organisations in a southern metropolitan Indian city formed the primary sample for the study. In addition, a group of 21 short-term volunteers, matched on age, income and gender, was utilised for comparison with long-term volunteers on well-being indices. A semi structured interview schedule was used to explore self-perceived changes attributable to volunteering experience. In addition, a few standardised measures were used to comprehensively assess subjective well-being and psychological well-being. The interview data provided rich descriptions of perceived positive changes in self across cognitive, behavioral and emotional domains. Mirroring these patterns, the quantitative analyses indicated that long-term volunteers experienced higher levels of psychological well-being (sense of mastery and competence, self-acceptance and sense of engagement and growth) than short-term volunteers. The potential mechanisms involved in beneficial outcomes of long-term volunteering and implications for further research are highlighted.
Binary Oxides with Defined Hierarchy of Pores in the Esterification of Glycerol
Authors: Alves Nuryana , Santiago Neto Antonio , Bessa Bruno , Oliveira Alcemira , Mendes Filho Josue , Campos Adriana , Oliveira Alcineia
Published: September 2016

Various porous binary oxides with elevated textural properties were obtained in this work. The as-synthesized solids were calcined or modified by reflux and extraction processes. Characterizations through SEM, nitrogen physisorption and TEM techniques demonstrated the formation of porous metal oxide networks over all solids. XRD, thermal analyses and FTIR measurements showed the existence of nanosized rutile TiO2, tetragonal ZrO2, SiO2 and γ-Al2O3 phases on the solids. The structure and texture of the as-synthesized SiAl sol-gel derived solid resulted in the formation of well-dispersed nanoparticles on the support. The removal of the organic compounds by ethanol extraction or reflux from SiAl resulted in the presence of structures with defined hierarchy of pores. Among the solids studied, the catalytic results in the esterification of glycerol with acetic acid indicated that best performances were obtained over SiAl sample when submitted to extraction and reflux treatments. This was due to the creation of accessible pores, which facilitated the reaction occurrence at glycerol to acetic acid molar ratio = 1:3 and T = 80 °C for 20 h using 75 mg of catalyst. The solids can be reused three times without complete loss of their catalytic performance.
Enhanced Removal of Trichloroethylene in Water Using Nano-ZnO/Polybutadiene Rubber Composites
Authors: Jang Dae , Ahn Chang , Choi June , Kim Jong , Kim Jong , Joo Jin
Published: September 2016

An innovative nanoscale ZnO/polybutadiene rubber composite (ZBRC) was developed as a valid alternative to TiO2 particles or immobilized TiO2 for the mineralization of chlorinated hydrocarbons without difficulties in the recovery of nanoscale photocatalyst particles. A synergistic increase in the removal of 1,1,2-trichloroethylene (TCE) through the coupled reaction processes (i.e., sorption, photolysis, and photocatalysis) was observed because sorption of TCE to the ultraviolet(UV)-transparent polybutadiene rubber occurred, and was coupled with the heterogeneous photocatalytic reactions with nanoscale ZnO particles on the surface of ZBRC. The removal rate of TCE decreased with an increase in the initial concentration of TCE because of both inhibited generation of electron–hole pairs and deficiency of photons to activate ZnO particles. Also, the TCE removal rate increased as the loading amount of ZBRC increased. Based on satisfactory linear regressions (R2 ≥ 0.94) between the apparent degradation rate constant (Kapp) and the initial concentration vs. the ZBRC loading amount, the Kapp values can be estimated, a priori, without performing photocatalytic experiments. The removal efficiencies were more significantly affected by the changes in the initial concentration of TCE and the ZBRC loading amounts than by the changes in light intensity and pH in aqueous solutions. From the results of response surface analysis, the greater removal efficiencies of TCE were achieved with higher pH values, greater amounts of ZBRC, and greater intensity of light. Based on these results, newly-developed ZBRC with both high removal efficiency and low cost performs as a valid alternative to TiO2 particles or immobilized TiO2 for the mineralization of chlorinated hydrocarbons in various environmental and industrial matrices.
Fuzzy Shannon Entropy: A Hybrid GIS-Based Landslide Susceptibility Mapping Method
Authors: Shadman Roodposhti Majid , Aryal Jagannath , Shahabi Himan , Safarrad Taher
Published: September 2016

Assessing Landslide Susceptibility Mapping (LSM) contributes to reducing the risk of living with landslides. Handling the vagueness associated with LSM is a challenging task. Here we show the application of hybrid GIS-based LSM. The hybrid approach embraces fuzzy membership functions (FMFs) in combination with Shannon entropy, a well-known information theory-based method. Nine landslide-related criteria, along with an inventory of landslides containing 108 recent and historic landslide points, are used to prepare a susceptibility map. A random split into training (≈70%) and testing (≈30%) samples are used for training and validation of the LSM model. The study area—Izeh—is located in the Khuzestan province of Iran, a highly susceptible landslide zone. The performance of the hybrid method is evaluated using receiver operating characteristics (ROC) curves in combination with area under the curve (AUC). The performance of the proposed hybrid method with AUC of 0.934 is superior to multi-criteria evaluation approaches using a subjective scheme in this research in comparison with a previous study using the same dataset through extended fuzzy multi-criteria evaluation with AUC value of 0.894, and was built on the basis of decision makers’ evaluation in the same study area.
Recognition of Abnormal Uptake through 123I-mIBG Scintigraphy Entropy for Paediatric Neuroblastoma Identification
Authors: Martínez-Díaz Milagros , Martínez-Díaz Rafael , Sánchez-Ruiz Luis , Peris-Fajarnés Guillermo
Published: September 2016

Whole-body 123I-Metaiodobenzylguanidine (mIBG) scintigraphy is used as primary image modality to visualize neuroblastoma tumours and metastases because it is the most sensitive and specific radioactive tracer in staging the disease and evaluating the response to treatment. However, especially in paediatric neuroblastoma, information from mIBG scans is difficult to extract because of acquisition difficulties that produce low definition images, with poor contours, resolution and contrast. These problems limit physician assessment. Current oncological guidelines are based on qualitative observer-dependant analysis. This makes comparing results taken at different moments of therapy, or in different institutions, difficult. In this paper, we present a computerized method that processes an image and calculates a quantitative measurement considered as its entropy, suitable for the identification of abnormal uptake regions, for which there is enough suspicion that they may be a tumour or metastatic site. This measurement can also be compared with future scintigraphies of the same patient. Over 46 scintigraphies of 22 anonymous patients were tested; the procedure identified 96.7% of regions of abnormal uptake and it showed a low overall false negative rate of 3.3%. This method provides assistance to physicians in diagnosing tumours and also allows the monitoring of patients’ evolution.
Geomechanical, Hydraulic and Thermal Characteristics of Deep Oceanic Sandy Sediments Recovered during the Second Ulleung Basin Gas Hydrate Expedition
Authors: Cha Yohan , Yun Tae , Kim Young , Lee Joo , Kwon Tae-Hyuk
Published: September 2016

This study investigates the geomechanical, hydraulic and thermal characteristics of natural sandy sediments collected during the Ulleung Basin gas hydrate expedition 2, East Sea, offshore Korea. The studied sediment formation is considered as a potential target reservoir for natural gas production. The sediments contained silt, clay and sand fractions of 21%, 1.3% and 77.7%, respectively, as well as diatomaceous minerals with internal pores. The peak friction angle and critical state (or residual state) friction angle under drained conditions were ~26° and ~22°, respectively. There was minimal or no apparent cohesion intercept. Stress- and strain-dependent elastic moduli, such as tangential modulus and secant modulus, were identified. The sediment stiffness increased with increasing confining stress, but degraded with increasing strain regime. Variations in water permeability with water saturation were obtained by fitting experimental matric suction-water saturation data to the Maulem-van Genuchen model. A significant reduction in thermal conductivity (from ~1.4–1.6 to ~0.5–0.7 W·m−1·K−1) was observed when water saturation decreased from 100% to ~10%–20%. In addition, the electrical resistance increased quasi-linearly with decreasing water saturation. The geomechanical, hydraulic and thermal properties of the hydrate-free sediments reported herein can be used as the baseline when predicting properties and behavior of the sediments containing hydrates, and when the hydrates dissociate during gas production. The variations in thermal and hydraulic properties with changing water and gas saturation can be used to assess gas production rates from hydrate-bearing deposits. In addition, while depressurization of hydrate-bearing sediments inevitably causes deformation of sediments under drained conditions, the obtained strength and stiffness properties and stress-strain responses of the sedimentary formation under drained loading conditions can be effectively used to assess sediment responses to depressurization to ensure safe gas production operations in this potential target reservoir.
Variability of Blazars and Blazar Models over 38 Years
Authors: Marscher Alan
Published: September 2016

Since 1978, when BL Lac objects and violently variable quasars were married to become “blazars”, physical interpretations of the phenomenon have evolved. Remarkably, though, the general picture of relativistic jets beaming their radiation in our direction, proposed that year by Blandford and Rees, remains intact. The main stress on theoretical models has come from observations that reveal ever more extreme variability requiring rampant particle acceleration on time-scales of minutes, often parsecs away from the central black hole. Here the author reviews many of the observations and theoretical ideas that have shaped his studies of blazars over about 40 years. This leads to his preferred scenario that blazar jets contain a helical magnetic field close to the black hole, turbulent plasma on parsecs scales, and both standing and moving shock waves. Particle acceleration can then occur in multiple stages involving the second-order Fermi process, magnetic reconnections, and modest jumps in energy at shock fronts. The most extreme variability, as well as brightness temperatures ~100 times the inverse Compton limit, probably require occasional exceptionally high bulk Lorentz factors. These can result, for example, from supersonic, relativistic turbulence, or ultra-relativistic flows propelled from sites of magnetic reconnection. Future efforts in these and other areas can determine whether these potential solutions are valid.
Polyelectrolyte Hydrogel Platforms for the Delivery of Antidepressant Drugs
Authors: Casolaro Mario , Casolaro Ilaria
Published: September 2016

Some vinyl hydrogels containing α-amino acid residues (l-phenylalanine, l-valine) were used as polyelectrolyte platforms for the evaluation of the controlled release of two antidepressants (paroxetine and duloxetine). The closer acidity constant (pKa) values of the two drugs show a closer release profile in physiological phosphate buffered saline (PBS) buffer (pH 7.40) and for long periods of time. The great electrostatic interaction forces between the COO− group of the hydrogel and the protonated secondary amino nitrogen of the drug are the main factor improving the release kinetics; this release was found to be slower compared to that of two structurally related drugs bearing the tertiary amino nitrogen atom (citalopram and trazodone). Moreover, at the lower value of pH 4.60, paroxetine showed a flatter release profile from the hydrogel containing the l-phenylalanine residues that, after six days, is half of that shown by duloxetine. Further effects due to steric and hydrophobic interactions may contribute to the different release profile. A further stimulation with alternating magnetic fields (AMF) of low frequency (20 kHz/50 W) enhanced the release of the drug at pH 7.40 from the hydrogel containing magnetic nanoparticles. Both AMF and PBS solution at pH 7.40 were used to trigger the ‘on-demand’ pulsatile paroxetine release from the nanocomposite hydrogel.
Assessing Floods and Droughts in Ungauged Small Reservoirs with Long-Term Landsat Imagery
Authors: Ogilvie Andrew , Belaud Gilles , Massuel Sylvain , Mulligan Mark , Le Goulven Patrick , Calvez Roger
Published: September 2016

Small reservoirs have developed across semi-arid areas as a low cost solution for millions of rural small holders to harvest scarce water resources. Studies have highlighted limited agricultural water use and low water availability on individual reservoirs, but no information exists on the drought patterns of multiple small reservoirs. Their small size and dispersion prevents individualised hydrological monitoring, while hydrological modelling suffers from rainfall variability and heterogeneity across data sparse catchments and reservoirs. A semi-automated original approach exploiting free, archive Landsat satellite images is developed here for long-term monitoring of multiple ungauged small water bodies. Adapted and tested against significant hydrometric time series on three lakes, the method confirms its potential to monitor water availability on the smallest water bodies (1–10 ha) with a mean RMSE of 20,600 m3 (NRMSE = 26%). Uncertainties from the absence of site-specific and updated surface-volume rating curves were here contained through a power relationship adapted over time for silting based on data from 15 surrounding lakes. Applied to 51 small reservoirs and 546 images over 1999–2014, results highlight the ability of this transposable method to shed light on flood dynamics and allow inter annual and inter lake comparisons of water availability. In the Merguellil upper catchment, in Central Tunisia, results reveal the significant droughts affecting over 80% of reservoirs, confirming the need for small reservoirs to maintain a supplementary irrigation objective only.
Post What? Disarticulating Post-Discourses in Toni Morrison’s God Help the Child
Authors: Gras Delphine
Published: September 2016

In the midst of the proliferation of post-discourses, this essay investigates how Toni Morrison’s God Help the Child (2015) offers a timely exploration of the hurting Black female body that calls into question, if not outright refutes, whether Americans have entered a post-racial, post-Black, and post-feminist era. This essay opens with a critical context section that situates God Help the Child within and against post-discourses, before examining how resemblances with Morrison’s prior works like Beloved (1987) and The Bluest Eye (1970) confirm that the legacy of slavery still dictates the way Black female bodies are seen and treated in twenty-first-century America. Ultimately, what this study intends is to speak the unspeakable: race still matters despite the silencing effects of post-discourses.
The Fairy Tale and Its Uses in Contemporary New Media and Popular Culture Introduction
Authors: Schwabe Claudia
Published: September 2016

Ever since the beginning of the 21st century, the fairy tale has not only become a staple of the small and silver screen around the globe, it has also migrated into new media, overwhelming audiences with imaginative and spectacular retellings along the way. Indeed, modern fairy-tale adaptations pervading contemporary popular culture drastically subvert, shatter, and alter the public’s understanding of the classic fairy tale. Because of the phenomenally increasing proliferation of fairy-tale transformations in today’s “old” and “new” media, we must reflect upon the significance of the fairy tale for society and its social uses in a nuanced fashion. How, why, and for whom have fairy-tale narratives, characters, and motifs metamorphosed in recent decades? What significant intermedial and intertextual relationships exist nowadays in connection with the fairy tale? This special issue features 11 illuminating articles of 13 scholars in the fields of folklore and fairy-tale studies tackling these and other relevant questions.
Automatic Scaling Hadoop in the Cloud for Efficient Process of Big Geospatial Data
Authors: Li Zhenlong , Yang Chaowei , Liu Kai , Hu Fei , Jin Baoxuan
Published: September 2016

Efficient processing of big geospatial data is crucial for tackling global and regional challenges such as climate change and natural disasters, but it is challenging not only due to the massive data volume but also due to the intrinsic complexity and high dimensions of the geospatial datasets. While traditional computing infrastructure does not scale well with the rapidly increasing data volume, Hadoop has attracted increasing attention in geoscience communities for handling big geospatial data. Recently, many studies were carried out to investigate adopting Hadoop for processing big geospatial data, but how to adjust the computing resources to efficiently handle the dynamic geoprocessing workload was barely explored. To bridge this gap, we propose a novel framework to automatically scale the Hadoop cluster in the cloud environment to allocate the right amount of computing resources based on the dynamic geoprocessing workload. The framework and auto-scaling algorithms are introduced, and a prototype system was developed to demonstrate the feasibility and efficiency of the proposed scaling mechanism using Digital Elevation Model (DEM) interpolation as an example. Experimental results show that this auto-scaling framework could (1) significantly reduce the computing resource utilization (by 80% in our example) while delivering similar performance as a full-powered cluster; and (2) effectively handle the spike processing workload by automatically increasing the computing resources to ensure the processing is finished within an acceptable time. Such an auto-scaling approach provides a valuable reference to optimize the performance of geospatial applications to address data- and computational-intensity challenges in GIScience in a more cost-efficient manner.
Indexing for Moving Objects in Multi-Floor Indoor Spaces That Supports Complex Semantic Queries
Authors: Lin Hui , Peng Ling , Chen Si , Liu Tianyue , Chi Tianhe
Published: September 2016

With the emergence of various types of indoor positioning technologies (e.g., radio-frequency identification, Wi-Fi, and iBeacon), how to rapidly retrieve indoor cells and moving objects has become a key factor that limits those indoor applications. Euclidean distance-based indexing techniques for outdoor moving objects cannot be used in indoor spaces due to the existence of indoor obstructions (e.g., walls). In addition, currently, the indexing of indoor moving objects is mainly based on space-related query and less frequently on semantic query. To address these two issues, the present study proposes a multi-floor adjacency cell and semantic-based index (MACSI). By integrating the indoor cellular space with the semantic space, the MACSI subdivides open cells (e.g., hallways and lobbies) using space syntax and optimizes the adjacency distances between three-dimensionally connected cells (e.g., elevators and stairs) based on the caloric cost that extends single floor indoor space to three dimensional indoor space. Moreover, based on the needs of semantic query, this study also proposes a multi-granularity indoor semantic hierarchy tree and establishes semantic trajectories. Extensive simulation and real-data experiments show that—compared with the indoor trajectories delta tree (ITD-tree) and the semantic-based index (SI)—the MACSI produces more reliable query results with significantly higher semantic query and update efficiencies; has superior semantic expansion capability; and supports multi-granularity complex semantic queries.
Preparation, Characterization and in Vivo Antimycobacterial Studies of Panchovillin-Chitosan Nanocomposites
Authors: Rwegasila Edward , Mubofu Egid , Nyandoro Stephen , Erasto Paul , Munissi Joan
Published: September 2016

Chitosan (CS, molecular weight 20.2 kDa, degree of deacylation (DD) 73.31%) was successfully obtained by deacetylation of chitin extracted from shrimp (Litopenaeus vannamei) shell wastes. The encapsulation of the bioactive natural product, panchovillin (PANV), isolated from Erythrina schliebenii, on a chitosan-tripolyphosphate (CS/TPP) nano-framework was achieved by ionotropic gelation. Characterization of pure CS, CS/TPP and PANV-CS/TPP nanocomposites was performed by FTIR, SEM and XRD. The molecular weight of chitosan and the thermal stability of the materials were determined by MALDI-TOF-MS and simultaneous thermal analyzer (STA)/DTG, respectively. The respective encapsulation efficiency and loading capacity of the PANV were found to be 70% and 0.36%. The in vitro release studies showed an initial burst of 42% of PANV in the first six hours. This was followed by a slow and sustained release up to 72 h. The in vivo antimycobacterial activities of both PANV and PANV-CS/TPP nanocomposite against Mycobacterium indicus pranii (MIP) using Galleria mellonella larvae as an in vivo infection model are reported in this paper.
Selenomethionine Ameliorates Neuropathology in the Olfactory Bulb of a Triple Transgenic Mouse Model of Alzheimer’s Disease
Authors: Zhang Zhong-Hao , Chen Chen , Wu Qiu-Yan , Zheng Rui , Chen Yao , Liu Qiong , Ni Jia-Zuan , Song Guo-Li
Published: September 2016

Olfactory dysfunction is an early and common symptom in Alzheimer′s disease (AD) and is reported to be related to several pathologic changes, including the deposition of Aβ and hyperphosphorylated tau protein as well as synaptic impairment. Selenomethionine (Se-Met), the major form of selenium in animals and humans, may be a promising therapeutic option for AD as it decreases the deposition of Aβ and tau hyperphosphorylation in a triple transgenic mouse model of AD (3× Tg-AD). In this study, 4-month-old AD mice were treated with 6 µg/mL Se-Met in drinking water for 12 weeks and the effect of Se-Met on neuropathological deficits in olfactory bulb (OB) of 3× Tg-AD mice was investigated. The administration of Se-Met effectively decreased the production and deposition of Aβ by inhibiting β-site amyloid precursor protein cleaving enzyme 1 (BACE1)-regulated amyloid precursor protein (APP) processing and reduced the level of total tau and phosphorylated tau, which depended on depressing the activity and expression of glycogen synthase kinase-3β (GSK-3β) and cyclin-dependent kinase 5 (CDK5). Meanwhile, Se-Met reduced glial activation, relieved neuroinflammation and attenuated neuronal cell death in the OB of AD mice. So Se-Met could improve pathologic changes of AD in the OB, which further demonstrated the potential therapeutic effect of Se-Met in AD.
Ocular Albinism Type 1 Regulates Melanogenesis in Mouse Melanocytes
Authors: Chen Tianzhi , Wang Haidong , Liu Yu , Zhao Bingling , Zhao Yuanyuan , Fan Ruiwen , Wang Pengchao , Dong Changsheng
Published: September 2016

To investigate whether ocular albinism type 1 (OA1) is differentially expressed in the skin of mice with different coat colors and to determine its correlation with coat color establishment in mouse, the expression patterns and tissue distribution characterization of OA1 in the skin of mice with different coat colors were qualitatively and quantitatively analyzed by real-time quantitative PCR (qRT-PCR), immunofluorescence staining and Western blot. The qRT-PCR analysis revealed that OA1 mRNA was expressed in all mice skin samples tested, with the highest expression level in brown skin, a moderate expression level in black skin and the lowest expression level in gray skin. Positive OA1 protein bands were also detected in all skin samples by Western blot analysis. The relative expression levels of OA1 protein in both black and brown skin were significantly higher than that in gray skin, but there was no significant difference between black and brown mice. Immunofluorescence assays revealed that OA1 was mainly expressed in the hair follicle matrix, the inner and outer root sheath in the skin tissues with different coat colors. To get further insight into the important role of OA1 in the melanocytes’ pigmentation, we transfected the OA1 into mouse melanocytes and then detected the relative expression levels of pigmentation-related gene. Simultaneously, we tested the melanin content of melanocytes. As a result, the overexpression of OA1 significantly increased the expression levels of microphthalmia-associated transcription factor (MITF), tyrosinase (TYR), tyrosinase-related protein 1 (TRP1) and premelanosome protein (PMEL). However, the tyrosinase-related protein 2 (TRP2) level was attenuated. By contrast, the level of glycoprotein non-metastatic melanoma protein b (GPNMB) was unaffected by OA1 overexpression. Furthermore, we observed a significant increase in melanin content in mouse melanocyte transfected OA1. Therefore, we propose that OA1 may participate in the formation of coat color by regulating the level of MITF and the number, size, motility and maturation of melanosome.
Isobaric Tags for Relative and Absolute Quantitation (iTRAQ)-Based Comparative Proteome Analysis of the Response of Ramie under Drought Stress
Authors: An Xia , Zhang Jingyu , Dai Lunjin , Deng Gang , Liao Yiwen , Liu Lijun , Wang Bo , Peng Dingxiang
Published: September 2016

In this study, we conducted the first isobaric tags for relative and absolute quantitation (isobaric tags for relative and absolute quantitation (iTRAQ))-based comparative proteomic analysis of ramie plantlets after 0 (minor drought stress), 24 (moderate drought stress), and 72 h (severe drought stress) of treatment with 15% (w/v) poly (ethylene glycol)6000 (PEG6000) to simulate drought stress. In our study, the association analysis of proteins and transcript expression revealed 1244 and 968 associated proteins identified in leaves and roots, respectively. L1, L2, and L3 are leaf samples which were harvested at 0, 24, and 72 h after being treated with 15% PEG6000, respectively. Among those treatment groups, a total of 118, 216, and 433 unique proteins were identified as differentially expressed during L1 vs. L2, L2 vs. L3, and L1 vs. L3, respectively. R1, R2, and R3 are root samples which were harvested at 0, 24, and 72 h after being treated with 15% PEG6000, respectively. Among those treatment groups，a total of 124, 27, and 240 unique proteins were identified as differentially expressed during R1 vs. R2, R2 vs. R3, and R1 vs. R3, respectively. Bioinformatics analysis indicated that glycolysis/gluconeogenesis was significantly upregulated in roots in response to drought stress. This enhancement may result in more glycolytically generated adenosine triphosphate (ATP) in roots to adapt to adverse environmental conditions. To obtain complementary information related to iTRAQ data, the mRNA levels of 12 proteins related to glycolysis/gluconeogenesis in leaves and 7 in roots were further analyzed by qPCR. Most of their expression levels were higher in R3 than R1 and R2, suggesting that these compounds may promote drought tolerance by modulating the production of available energy.
Elevated Expression of Calpain-4 Predicts Poor Prognosis in Patients with Gastric Cancer after Gastrectomy
Authors: Peng Peike , Min Lingqiang , Song Shushu , Zhao Junjie , Li Lili , Yang Caiting , Shao Miaomiao , Zhang Mingming , Wu Hao , Zhang Jie , Li Can , Wang Xuefei , Wang Hongshan , Qin Jing , Ruan Yuanyuan , Gu Jianxin
Published: September 2016

Calpain-4 belongs to the calpain family of calcium-dependent cysteine proteases, and functions as a small regulatory subunit of the calpains. Recent evidence indicates that calpain-4 plays critical roles in tumor migration and invasion. However, the roles of calpain-4 in gastric tumorigenesis remain poorly understood. Herein, we examined calpain-4 expression by immunohistochemical staining on tissue microarrays containing tumor samples of 174 gastric cancer patients between 2004 and 2008 at a single center. The Kaplan-Meier method was used to compare survival curves, and expression levels were correlated to clinicopathological factors and overall survival. Our data demonstrated that calpain-4 was generally increased in gastric cancer cell lines and primary tumor tissues. High expression of calpain-4 was positively associated with vessel invasion, lymph node metastasis, and advanced TNM (Tumor Node Metastasis) stage. Multivariate analysis identified calpain-4 as an independent prognostic factor for poor prognosis. A predictive nomogram integrating calpain-4 expression with other independent prognosticators was constructed, which generated a better prognostic value for overall survival of gastric cancer patients than a TNM staging system. In conclusion, calpain-4 could be regarded as a potential prognosis indicator for clinical outcomes in gastric cancer.
MicroRNA-375 Functions as a Tumor-Suppressor Gene in Gastric Cancer by Targeting Recepteur d’Origine Nantais
Authors: Lian Sen , Park Jung , Xia Yong , Nguyen Thi , Joo Young , Kim Kyung , Kim Hark , Jung Young
Published: September 2016

Emerging evidence supports a fundamental role for microRNAs (miRNA) in regulating cancer metastasis. Recently, microRNA-375 (miR-375) was reported to be downregulated in many types of cancers, including gastric cancer. Increase in the expression of Recepteur d’Origine Nantais (RON), a receptor tyrosine kinase, has been reported in tumors. However, the function of miR-375 and RON expression in gastric cancer metastasis has not been sufficiently studied. In silico analysis identified miR-375 binding sites in the 3′-untranslated regions (3′-UTR) of the RON-encoding gene. Expression of miR-375 resulted in reduced activity of a luciferase reporter containing the 3′-UTR fragments of RON-encoding mRNA, confirming that miR-375 directly targets the 3′-UTR of RON mRNA. Moreover, we found that overexpression of miR-375 inhibited mRNA and protein expression of RON, which was accompanied by the suppression of cell proliferation, migration, and invasion in gastric cancer AGS and MKN-28 cells. Ectopic miR-375 expression also induced G1 cell cycle arrest through a decrease in the expression of cyclin D1, cyclin D3, and in the phosphorylation of retinoblastoma (Rb). Knockdown of RON by RNAi, similar to miR-375 overexpression, suppressed tumorigenic properties and induced G1 arrest through a decrease in the expression of cyclin D1, cyclin D3, and in the phosphorylation of Rb. Thus, our study provides evidence that miR-375 acts as a suppressor of metastasis in gastric cancer by targeting RON, and might represent a new potential therapeutic target for gastric cancer.
Urine Levels of Defensin α1 Reflect Kidney Injury in Leptospirosis Patients
Authors: Chagan-Yasutan Haorile , Chen Yue , Lacuesta Talitha , Leano Prisca , Iwasaki Hiroko , Hanan Firmanto , Taurustiati Delsi , Ohmoto Yasukazu , Ashino Yugo , Saitoh Hiroki , Kiyomoto Hideyasu , Suzuki Yasuhiko , Telan Freda , Hattori Toshio
Published: September 2016

Leptospirosis is a zoonotic disease whose severe forms are often accompanied by kidney dysfunction. In the present study, urinary markers were studied for potential prediction of disease severity. Urine samples from 135 patients with or without leptospirosis at San Lazaro Hospital, the Philippines, were analyzed. Urine levels of defensin α1 (uDA1) were compared with those of neutrophil gelatinase-associated lipocalin (uNGAL) and N-acetyl-β-d-glucosidase (uNAG). Serum creatinine (Cr) was used as a marker of kidney injury. The levels of uDA1/Cr, uNGAL/Cr, and uNAG/Cr were positive in 46%, 90%, and 80% of leptospirosis patients, and 69%, 70%, and 70% of non-leptospirosis patients, respectively. In leptospirosis patients, the correlation of uDA1/Cr, uNGAL/Cr and uNAG/Cr levels with serum Cr were r = 0.3 (p < 0.01), r = 0.29 (p < 0.01), and r = 0.02 (p = 0.81), respectively. uDA1/Cr levels were correlated with uNGAL/Cr levels (r = 0.49, p < 0.01) and uNAG/Cr levels (r = 0.47, p < 0.0001) in leptospirosis patients. These findings suggest that uDA1, uNGAL, and uNAG were elevated in leptospirosis patients and reflected various types of kidney damage. uDA1 and uNGAL can be used to track kidney injury in leptospirosis patients because of their correlation with the serum Cr level.
Erosion Behaviour of API X100 Pipeline Steel at Various Impact Angles and Particle Speeds
Authors: Okonkwo Paul , Shakoor Rana , Zagho Moustafa , Mohamed Adel
Published: September 2016

Erosion is the gradual removal of material due to solid particle impingement and results in a failure of pipeline materials. In this study, a series of erosion tests were carried out to investigate the influence of particle speed and impact angle on the erosion mechanism of API X100 pipeline steel. A dry erosion machine was used as the test equipment, while the particle speed ranged from 20 to 80 m/s and impact angles of 30° and 90° were used as test parameters. The eroded API X100 steel surface was characterized using scanning electron microscope (SEM) and X-ray photoelectron spectroscopy (XPS). The weight loss and erosion rate were also investigated. The results showed that at a 90° impact angle, a ploughing mechanism was occurring on the tested specimens, while material removal through low-angle cutting was the dominant mechanism at lower impact angles. Embedment of alumina particles on the target steel surface, micro-cutting, and low-angle cutting were observed at low impact angles. Therefore, the scratches, cuttings, and severe ploughings observed on some failed oil and gas pipelines could be attributed to the erosion mechanism.
Cordycepin Induces Apoptosis and Inhibits Proliferation of Human Lung Cancer Cell Line H1975 via Inhibiting the Phosphorylation of EGFR
Authors: Wang Zheng , Wu Xue , Liang Yan-Ni , Wang Li , Song Zhong-Xing , Liu Jian-Li , Tang Zhi-Shu
Published: September 2016

Cordycepin is an active component of the traditional Chinese medicine Cordyceps sinensis and Cordyceps militaris with notable anticancer activity. Though the prominent inhibitory activity was reported in different kinds of cancer cell lines, the concrete mechanisms remain elusive. It was reported that cordycepin could be converted into tri-phosphates in vivo to confuse a number of enzymes and interfere the normal cell function. For the inhibitory mechanism of EGFR inhibitors and the structure similarity of ATP and tri-phosphated cordycepin, human lung cancer cell line H1975 was employed to investigate the inhibitory effect of cordycepin. The results showed that cordycepin could inhibit cell proliferation and induce apoptosis in a dose-dependent manner. Cell cycle analysis revealed that H1975 cells could be arrested at the G0/G1 phase after cordycepin treatment. The expression levels of apoptosis-related protein Caspase-3 and Bcl-2 and phosphorylated expression levels of EGFR, AKT and ERK1/2 were all decreased compared with the control group stimulated with EGF. However, the protein expression levels of proapoptotic protein Bax and cleaved caspase-3 were increased. These results implied that cordycepin could inhibit cell proliferation and induce apoptosis via the EGFR signaling pathway. Our results indicated that there was potential to seek a novel EGFR inhibitor from cordycepin and its chemical derivatives.
The Growth of SGC-7901 Tumor Xenografts Was Suppressed by Chinese Bayberry Anthocyanin Extract through Upregulating KLF6 Gene Expression
Authors: Wang Yue , Zhang Xia-nan , Xie Wen-hua , Zheng Yi-xiong , Cao Jin-ping , Cao Pei-rang , Chen Qing-jun , Li Xian , Sun Chong-de
Published: September 2016

To investigate the antitumor effect of anthocyanins extracted from Chinese bayberry fruit (Myrica rubra Sieb. et Zucc.), a nude mouse tumor xenograft model was established. Treatments with C3G (cyanidin-3-glucoside, an anthocyanin) significantly suppressed the growth of SGC-7901 tumor xenografts in a dose-dependent manner. Immunohistochemical staining showed a significant increase in p21 expression, indicating that the cell cycle of tumor xenografts was inhibited. qPCR screening showed that C3G treatment up-regulated the expression of the KLF6 gene, which is an important tumor suppressor gene inactivated in many human cancers. Western blot showed that C3G treatments markedly increased KLF6 and p21 protein levels, inhibited CDK4 and Cyclin D1 expression, but did not notably change the expression of p53. These results indicated that KLF6 up-regulates p21 in a p53-independent manner and significantly reduces tumor proliferation. This study provides important information for the possible mechanism of C3G-induced antitumor activity against gastric adenocarcinoma in vivo.
Lactoferrin from Milk: Nutraceutical and Pharmacological Properties
Authors: Giansanti Francesco , Panella Gloria , Leboffe Loris , Antonini Giovanni
Published: September 2016

Lactoferrin is an iron-binding protein present in large quantities in colostrum and in breast milk, in external secretions and in polymorphonuclear leukocytes. Lactoferrin’s main function is non-immune protection. Among several protective activities shown by lactoferrin, those displayed by orally administered lactoferrin are: (i) antimicrobial activity, which has been presumed due to iron deprivation, but more recently attributed also to a specific interaction with the bacterial cell wall and extended to viruses and parasites; (ii) immunomodulatory activity, with a direct effect on the development of the immune system in the newborn, together with a specific antinflammatory effects; (iii) a more recently discovered anticancer activity. It is worth noting that most of the protective activities of lactoferrin have been found, sometimes to a greater extent, also in peptides derived from limited proteolysis of lactoferrin that could be generated after lactoferrin ingestion. Lactoferrin could therefore be considered an ideal nutraceutic product because of its relatively cheap production from bovine milk and of its widely recognized tolerance after ingestion, along with its well demonstrated protective activities. The most important protective activities shown by orally administered bovine lactoferrin are reviewed in this article.
Introduction to the Special Issue: “The Wayfinders: Why Ancient Wisdom Matters in the Modern World”, Religions 2016
Authors: Keefer Arthur , Dell Katharine
Published: September 2016

In “The Wayﬁnders”, a Special Issue for the journal Religions, scholars explore the signiﬁcance of the biblical wisdom literature for the current day.[...]
Terrain Perception in a Shape Shifting Rolling-Crawling Robot
Authors: Masataka Fuchida , Mohan Rajesh , Tan Ning , Nakamura Akio , Pathmakumar Thejus
Published: September 2016

Terrain perception greatly enhances the performance of robots, providing them with essential information on the nature of terrain being traversed. Several living beings in nature offer interesting inspirations which adopt different gait patterns according to nature of terrain. In this paper, we present a novel terrain perception system for our bioinspired robot, Scorpio, to classify the terrain based on visual features and autonomously choose appropriate locomotion mode. Our Scorpio robot is capable of crawling and rolling locomotion modes, mimicking Cebrenus Rechenburgi, a member of the huntsman spider family. Our terrain perception system uses Speeded Up Robust Feature (SURF) description method along with color information. Feature extraction is followed by Bag of Word method (BoW) and Support Vector Machine (SVM) for terrain classification. Experiments were conducted with our Scorpio robot to establish the efficacy and validity of the proposed approach. In our experiments, we achieved a recognition accuracy of over 90% across four terrain types namely grass, gravel, wooden deck, and concrete.