bluuebunny/crossref_metadata_2025 huggingface dataset:
Towards Understanding the Mechanisms of Gene Expression in Tetrahymena Thermophila
Authors: Petrova Alexandra
Published: September 2024

Gene regulation in eukaryotes is highly complex, and our understanding of many mechanisms remains limited, particularly in divergent eukaryotes. One of the key proteins in eukaryotic transcription is RNA polymerase II (RNAPII), an enzyme that transcribes genes to produce a functional product. Regulation of transcription is accomplished through many mechanisms, for instance, by utilizing histone variants to control recruitment of the transcriptional machinery and its access to DNA-binding sites. Histone variants H3.3 and H2A.Z are typically associated with active transcription. H3.3 deposition is usually observed in promoters and gene bodies, whereas H2A.Z is typically found in well-positioned nucleosomes around the transcription start sites. This project aimed to establish DNA-binding patterns of the third largest RNAPII subunit, Rpb3, and H3.3 in a model ciliate and divergent eukaryote Tetrahymena thermophila by utilizing next-generation sequencing and computational approaches. While Rpb3 research is still ongoing, I examined H3.3 deposition patterns and found the enrichment to be particularly strong in gene bodies and around transcription termination sites of genes involved in essential cellular processes. H3.3 also had an unexpected effect on the expression of DNA replication and chromatin organization genes, which requires further investigation. Furthermore, I examined the binding patterns of bromodomain-containing protein Ibd2, which was thought to have a role in H2A.Z removal by a chromatin-associated complex INO80. The analysis revealed that Ibd2 exclusively localized to H2A.Z-enriched genes, which provides further evidence for the current model of H2A.Z deposition pathway. Collectively, these findings contributed to a better understanding of gene regulation in protists. Furthermore, Tetrahymena shares a lot of conserved gene regulation mechanisms with higher eukaryotes. Therefore, these findings may be applicable to metazoans, including humans.
Motion Prediction Using LSTM Network in a Vehicular Environment
Authors: Freire Andrei Walter
Published: September 2024

Autonomous driving has been rapidly increasing in demand and interest over the past several years. It is critical to understand if a pedestrian/cyclist is about to cross the road or if a car is in the middle of parallel parking or making a right turn. There are hundreds of possible actions a road user can take and it is imperative to accurately predict the behaviour of other road users which makes this a challenging problem in autonomous driving. Correctly predicting and evaluating other road users' movements is essential to being able to reduce and avoid crashes thus improving the safety of autonomous vehicles. This MRP solves a part of the Waymo motion prediction challenge using the Waymo motion dataset. The problem is to predict 8 seconds into the future given 1 second of history. The work in this MRP uses (LSTM) Long Short Term Memory networks which is a special type of (RNN) Recurrent Neural Network, which have been widely used for time series prediction such as stock price prediction and electric load forecasting. This problem has been approached by many researchers using different machine learning algorithms such as (CNN) Convolutional Neural Network, LSTM and encoders/decoders. We found that LSTM networks can outperform several state-of-the-art approaches without the use of all available features. Surprisingly, we found that only using the agents' location, speed and angle was sufficient to get state-of-the-art results with a simple LSTM model and data preprocessing. The results obtained from the proposed solution outperformed current LSTM-based methods. The proposed method is implemented in python using the Tensorflow/Keras framework and is available in GitHub https://github.com/wfrei020/motion-prediction-v1. The MRP presents results from eight different road scenarios.
Integration of Solar Photovoltaic Systems in Medium-Voltage DC Networks Using Modular DC Converters
Authors: Kaler Sandeep
Published: September 2024

Solar photovoltaic (PV) energy systems have been most commonly constructed based on ac technologies. The recent visions of multi-terminal direct-current (MTdc) grids, dc distribution systems for densely populated urban areas, and dc microgrids for more convenient integration of distributed energy resources (including renewable energies, electric vehicles, and energy storage devices such as batteries) have motivated the shift towards solar PV power systems based on dc technologies. A key enabling technology required for such a change in the status quo are dc/dc power electronic converters that are capable of efficiently integrating low-voltage (LV) solar PV farms into medium-voltage (MV) dc networks. This dissertation, therefore, proposes the use of a modular dc transformer (DCT) based on the dual active bridge (DAB) converter and modular multilevel converter (MMC) to facilitate the integration of solar PV sources in MVdc networks. The proposed DCT employs a Voltage Matching Scheme (VMS) to address the issues of high current stress and reactive power losses that plague DAB-based converter topologies in the presence of unmatched transformer terminal voltages. The DCT is analyzed in detail to model various aspects of the converter such as the power throughput and zero-voltage switching regions, while also providing insight on more practical matters such as the sizing of passive components and their impact on converter operation. A novel modular multiport converter (MPC) is then derived based on the DCT to allow for the integration of both solar PV sources and battery energy storage through a single power conversion stage. It is shown that the MPC is capable of extracting power from the PV sources using maximum-power-point-tracking (MPPT) over a wide range of operating points. The MPC also employs the VMS to minimize current stress on the transformer. The developed models are all verified through simulation studies based on the Mat-lab & Simulink software environment, a 1.2-kW experimental DCT prototype, and a 550-W experimental MPC prototype. Both prototypes are shown to have efficiencies exceeding 97%.
إدارة الاحتواء وعلاقتها بتعزيز العمل الجماعي لأعضاء الهيئات الادارية للاتحادات الرياضية المركزية في العراق
Authors: Dr. Azhar Abid Mohammed
Published: September 2024

هدفت الدراسة الى التعرف على إدارة الاحتواء وعلاقتها بتعزيز العمل الجماعي لدى أعضاء الهيئات الادارية للاتحادات الرياضية المركزية في العراق، واستخدمت الباحثة المنهج الوصفي بالأَسلوب المسحي والعلاقات الارتباطية، وحددت الباحثة عينة البحث التي تمثلت بأعضاء الهيئات الإدارية للاتحادات الرياضية المركزية في العراق، والبالغ عددهم (347) فرداً موزعين على (49) اتحاد في العراق، ثم قامت الباحثة باتباع كافة الإجراءات العلمية من صدق وثبات وقدرة تميزية واتساق داخلي في( بناء مقياس إدارة الاحتواء), و(اعداد مقياس العمل الجماعي)، والتي نتج منها مقياسا الدراسة بصيغتهما النهائية، إذ احتوى مقياس إدارة الاحتواء على (16) فقرة، وموزع بمجالاته هي (الاندماج الوظيفي, التدريب, التحفيز والمكافئات, دعم تكنلوجيا العمل)، ومقياس العمل الجماعي على (24) فقرة، وموزع بمجالاته هي( التعاون, المحبة, النفسي, الاجتماعي) ,بعد ذلك قامت الباحثة بتطبيق مقياسي الدراسة على عينة التطبيق وإِجراء كافة الوسائل الإِحصائية ذات العلاقة بأهداف ومتغيرات البحث، وكانت اهم استنتاجات البحث هو تمتع الهيئات  الإدارية للاتحادات الرياضية المركزية في العراق تمتع الاتحادات الرياضية بحسن إدارة الاحتواء، ممزوجة بروح العمل الجماعي، ووجود علاقة ارتباطية جيدة بين إدارة الاحتواء والعمل الجماعي، التي تساعد على سرعة انجاز كافة المهام الإدارية في الاتحادات الرياضية المركزية في العراق.
تأثير تدريبات أسلوب المربعات الأربعة في بعض القدرات البدنية والمهارية والوظيفية للاعبي مركز اكتشاف اللاعب الموهوب بأعمار 10-12 سنة لكرة القدم
Authors: Prof.Dr.FADHIL DAHAM MANSOUR  , ALI HUSSEIN SAGHEER Asst. Dr  , HUMAM ISMAEL HUSSEIN Dr.
Published: September 2024

تضمنت مشكلة البحث في وجود  إنخفاضاً  واضحا في مستوى  القدرات البدنية, وخاصة القدرة الانفجارية ، وتحمل الاداء وعدم امتلاك اللاعبين التوافق والتوازن في أداء الحركات والمهارات الأساسية من خلال عدم مقدرة اللاعبين على الأداء القوي, والسريع طوال فترة المباراة فالتدريب بكرة القدم هو لعب يستهدف كافة الإبعاد  لذا يسعى الباحثون لحل هذه المشكلة باستعمال أسلوب  (المربعات الاربعة), وهدفت الدراسة إلى إعداد برنامج تدريبي بأسلوب المربعات الاربعة لتطوير بعض القدرات البدنية (القدرة الانفجارية ، وتحمل الاداء) والمهارات (المناولة، الدحرجة , التهديف) والمتغيرات الوظيفية (السعة الحيوية  والحد الاقصى لاستهلاك الاوكسجين )للاعبي مركز اكتشاف اللاعب الموهوب لكرة القدم بأعمار 10 -12سنة , والتعرّف على تأثير البرنامج التدريبي في المتغيرات المبحوثة,  كما إستعمل الباحثون المنهج التجريبي بأسلوب المجموعتين   ( التجريبية والضابطة) وتم إجراء الإختبارات القبلية لمتغيرات البحث القدرات البدنية (القدرة الانفجارية ، وتحمل الاداء) المهارات  الاساسية (المناولة، والدحرجة) والمتغيرات الوظيفية (السعة الحيوية) وتم تطبيق البرنامج خلال التجربة الرئيسة على المجموعة التجريبية  خلال (24), وحدة تدريبية في (8) أسابيع, أي بواقع (3) وحدات تدريبية في الأسبوع  , وتم إجراء الإختبارات البعدية لمعرفة التطور الحاصل في متغيرات البحث من خلال الموازنة بين نتائج الإختبارات القبلية, والبعدية, واستنتج الباحثون إنً أسلوب المربعات الاربعة أسهم في تطوير متغيرات البحث ،كما استنج الباحثون ان اسلوب المربعات الاربعة  اكثر ملائمة للفئات العمرية الصغيرة(البراعم)
Penerapan Prinsip 5C dalam Pemberian Kredit di Lembaga Perkreditan Desa: Studi Kasus Desa Adat Kukuh
Authors: Sari Kadek Dego Nyampuh Wijaya Satria , Mahaputra I Nyoman Kusuma Adnyana , Yuesti Anik , Mariati Ni Putu Ayu Mirah , Rustiarini Ni Wayan
Published: September 2024

Lembaga Perkreditan Desa (LPD) memegang peranan penting dalam menggerakkan perekonomian rakyat di Provinsi Bali. Kredit bermasalah menjadi persoalan keuangan yang sering dihadapi LPD, termasuk LPD Desa Adat Kukuh. Kegiatan pelatihan analisis kredit yang didasarkan pada prinsip 5C penting dilakukan untuk menyelesaikan problem tersebut. Pelaksanaan kegiatan bertujuan untuk meningkatkan kompetensi sumber daya manusia pengelola LPD, terutama dalam menganalisis proposal kredit. Kegiatan pengabdian dilakukan di LPD Desa Adat Kukuh, Kabupaten Tabanan. Pelaksanaan kegiatan menggunakan metode wawancara, pelatihan, dan evaluasi. Evaluasi efektivitas kegiatan pengabdian kepada masyarakat menunjukkan adanya peningkatan kompetensi peserta sebesar 8,73%. Angka tersebut menunjukkan bahwa pelatihan analisis kredit terbukti meningkatkan kompetensi pegawai. Dengan demikian, kegiatan ini telah berjalan secara efektif.
PELATIHAN PEMBUATAN SABUN CUCI CAIR PADA WARGA KAMPUNG BULAK PONDOK MELATI KOTA BEKASI
Authors: Mayarni  , Kartikawati Eka , Murwitaningsih Susanti
Published: September 2024

The partner's problem is the lack of creativity to start an entrepreneurship. Partners include residents who are dominated by the lower middle economy. The survey results show that the residents work as teachers, office workers, factory workers selling snacks but this is only about five percent of the total local population. Other professions such as washing and scrubbing, traveling vegetable sellers, and construction workers are fewer than those who are unemployed. Furthermore, there are families who have the skills to make snacks such as chips, pecal and fried foods, but there are only about three to five families.  This means that the local community, especially housewives, is dominated by unemployed mothers who are supported by their husbands' income, which is dominated by irregular income. This problem has an impact on the disruption of children's schooling, domestic quarrels and even divorce. The solution that will be offered is to increase family income by entrepreneurship through making liquid laundry soap. Making this liquid laundry soap is not too difficult and the raw materials are also easy to obtain. The service method uses training and demonstration. The results of community service activities in making liquid laundry soap are very necessary, this is because it can be used as an alternative side entrepreneurship that is very profitable for the community.
PENGUATAN KOMPETENSI PERSONIL: PELATIHAN PENULISAN KARYA TULIS ILMIAH BAGI TNI SATJAR BRIGIF PARA RAIDER 3/TBS KOSTRAD KARIANGO MAROS
Authors: Gustaf Said Try , Satriani Is Sitti , Agustan  , B Syamsuddin Yati , Baharuddin  , Carsel Syamsuni
Published: September 2024

Scientific papers are generally carried out by academics, but over time in the 21st century today the writing of scientific papers is also required in State defense institutions such as the TNI. Writing scientific papers is relatively new for the TNI, therefore intense, systematic and planned training is needed to provide in-depth knowledge about the procedures for writing scientific papers to the TNI. At Kostrad Kariango Maros is located in Sudirman Village, Tanra Lili District, Maros Regency, at Kostrad, Kariango 1 battalion consists of 2500 personnel, but the focus of the PKM is TNI personnel who serve as staff, non-commissioned officers and officers in the Brigif Denma unit. The purpose of implementing community service is to provide theory to the TNI about the importance of writing scientific papers, qualitative research material in writing scientific papers, training in good and correct article writing ethics, reviewing the use of good and correct Indonesian in writing scientific papers, and training in mendeley in writing scientific papers. The stages of activities proposed in the implementation of the PKM are the preparation stage, assessment stage, alternative activity planning stage, formulation stage, action plan, activity implementation stage, evaluation stage, and termination stage. The output of the PKM is in the form of youtube, newspapers, PKM is published in accredited national journals. The implementation of the PKM went according to plan. The PKM results show that scientific writing training for TNI Satjar personnel of Brigif Para Raider 3/TBS Kostrad Kariango Maros has an important role in increasing the professionalism and competence of personnel.
PELATIHAN KESEKRETARIATAN BAGI PENGURUS PIMPINAN DAERAH ‘AISYIYAH (PDA) KABUPATEN TAKALAR
Authors: Ratnawati  , Mutmainna  , Satriani Is Sitti
Published: September 2024

The Regional Executive of 'Aisyiah of Takalar district has recently carried out the inauguration and strengthening of the Muhammadiyah ideology to the new management of the PDA of Takalar district. This is the attraction of the candidate for the head of the Unismuh Makassar PkM Team who is also the deputy secretary of the PWA Culture, Arts and Sports Institute (LBSO) to conduct a secretarial training for all administrators or assemblies in the management of PDA Takalar district. Secretarial science is a very important part to be owned by every 'Aisyiyah organization administrator, both in the 'Aisyiyah Regional Executive (PWA), the 'Aisyiyah Regional Executive (PDA), the 'Aisyiyah Branch Executive (PCA), and the 'Aisyiyah Charity (AUA) in every region in South Sulawesi. The organizational apparatus of 'Aisyiyah, the Administration of the Secretariat of 'Aisyiyah, and the trial procedures at 'Aisyiyah are part of the science of secretarial. This is considered very important to know and be able to implement its knowledge in every implementation of the work program that has been agreed upon at the 2023 South Sulawesi PWA Regional Working Meeting (RAKERWIL) meeting organized by each assembly or institution
CORE VALUES AND EMPLOYER BRANDING ARE MORAL TO IMPROVE ISLAMIC SERVICES IN THE JANUR KUNING HOME INDUSTRY IN REJING VILLAGE, PROBOLINGGO REGENCY
Authors: A'yun Qurrota
Published: September 2024

This research aims to analyze the relationship between the application of core values and employer branding strategies in improving Islamic services in the Janur Kuning home industry in Rejing Village, Probolinggo Regency. The Janur Kuning home industry has become an integral part of the culture and economy of the local community, with a significant contribution to local economic development. This research method uses a qualitative approach through in-depth interviews and participatory observation of business owners and employees of the Janur Kuning home industry. This research also involves analysis of documentation related to the core values implemented in the business and employer branding strategies that have been carried out. The research results show that the application of core values, such as honesty, courage, friendliness and justice, has become a strong basis for the work culture in the Janur Kuning home industry. The application of these values does not only affect the internal relationship between business owners and employees. However, it also has an impact on interactions with customers and society in general. Apart from that, the employer branding strategy which focuses on developing the company's image as an Islamic and value-oriented workplace has helped improve the Islamic services provided by the Janur Kuning home industry. Steps such as training employees in Islamic values. Implementation of fair and transparent work patterns, as well as involvement in Islamic social activities which have improved the company's reputation in the eyes of customers and the community. In conclusion, the application of core values and employer branding strategies that focus on Islam has helped the Janur Kuning home industry to improve their Islamic services. The implication of this research is the importance of strengthening a company culture based on Islamic values, as well as developing an Islamic company image in an effort to increase customer trust and loyalty.
EL CUPLÉ LA VIOLETERA EN EL CINE INTERNACIONAL: DEL TEATRO A LA GRAN PANTALLA
Authors: Navas López Francisco , Galiano-Díaz Juan Carlos
Published: September 2024

El cuplé es un tipo de espectáculo musical que alcanzó gran popularidad en la España de comienzos del siglo XX. Ahondando en lo anterior, el compositor José Padilla constituye uno de los mayores exponentes del cuplé en España. Prueba de ello son las más de 300 obras pertenecientes a este género que compuso, entre las que destaca La Violetera (1914). Dado lo expuesto, el presente texto aspira a estudiar la presencia del mencionado cuplé en tres casos de estudio de filmes internacionales —Luces de la Ciudad (1931), Esencia de Mujer (1992) y Aprendiz de Gigoló (2013)—, haciendo hincapié en el análisis y funciones que este cumple como parte de su Banda Sonora Musical (BSM). La metodología empleada parte de las teorías enjuiciadas por Lluis i Falcó (1995), Fraile Prieto (2004) y Tagg (2013). Los resultados obtenidos muestran que el cuplé de Padilla presenta unos usos y funciones específicas en cada uno de los filmes analizados que lo alejan una vinculación a la representación de localización vinculada con el estereotipo cultural español.
DECONSTRUYENDO IMÁGENES Y TRANSFORMANDO HISTORIAS: MUJERES QUE DESAFÍAN ESTEREOTIPOS DE GÉNERO EN LAS PRÁCTICAS ARTÍSTICAS
Authors: Gómez Díaz Francisco José
Published: September 2024

Esta investigación se sumerge en el videoarte como medio de expresión y disrupción de estereotipos de género, centrándose en la obra de mujeres artistas. Se analiza la convergencia entre el videoarte y la redefinición de roles de género, explorando cómo las mujeres artistas utilizan este medio para desafiar y deconstruir estereotipos arraigados. Así mismo, busca profundizar en este fenómeno, destacando el papel crítico del videoarte en la transformación de narrativas y percepciones de género.
A Boa Nova: Uma Igreja Medieval no Pará do século XIX – O projeto teológico-doutrinário do bispo Dom Macedo por intermédio do neomedievalismo
Authors: Silva Dos Santos Mayara Fernanda
Published: September 2024

No Pará do Brasil, na segunda metade do século XIX, o bispo diocesano Dom Antônio de Macedo Costa empreende uma grande obra física, iconográfica e teológico-doutrinária na Catedral da Sé de Belém e em seu bispado. O que parece ser uma simples reforma se revela numa busca por (re)construir uma Igreja Medieval. Por meio do jornal diocesano A Boa Nova (1871-1833) e outras publicações de sua autoria ou direção, observaremos como o bispo Dom Macedo forja um projeto teológico-doutrinário medievalizante para o bispado do Pará. Analisando o seu discurso, por intermédio do neomedievalismo, podemos observar que ele se opõe à modernidade do século XIX e busca se alinhar à Idade Média, tendo-a como fonte de legitimidade, identidade e auctoritas. As apropriações que Dom Macedo faz do medievo o levam na direção de (re)criar teológica e doutrinariamente uma Igreja Medieval no Pará.
Artal Maillie, Susana G. (ed.). Alfred, Lord Tennyson. El último torneo. Proyecciones anglófonas de la leyenda de Tristán e Iseo, Buenos Aires, Dedalus, 2023, 186 pp.
Authors: Lacalle Juan Manuel
Published: September 2024

.
Pasado y futuro del neomedievalismo “fuera de lugar”
Authors: Altschul Nadia
Published: September 2024

¿Qué significa la Edad Media fuera de Europa, si Europa es el lugar directamente asociado con su origen y pertenencia? ¿Qué hacer con una Edad Media que, siguiendo el conocido término de Roberto Schwartz sobre las “ideas fuera de lugar”, está allí donde aparentemente no le corresponde? ¿Qué explicación podemos considerar para este trance de aparente inadecuación a ciertas geografías? El neomedievalismo es una aproximación a la Edad Media retomada después del Medioevo histórico y, en ese sentido, se lo puede considerar como una Edad Media “fuera de tiempo”. El presente artículo trabajará desde un enfoque teórico, con especial atención a cuestiones terminológicas y conceptuales, las características y posibilidades del neomedievalismo en Iberoamérica.
Transformation of small business taxation - lessons for Ukraine: a study of the experience of EU countries with transitional economies
Authors:   SESE “The Academy of Financial Management”, Oleinikova Liudmyla
Published: September 2024

Introduction. The significance of the simplified taxation system (STS) on the socio-economic processes in the micro-business sector cannot be overstated, especially in the context of the outflow of citizens abroad due to the war. Taxation of the smallest and most vulnerable business entities is an important component of the state’s tax policy. Problem Statement. In Ukraine, in conditions of full-scale Russian aggression there is a pressing need to align the tax policy for small business taxation with the National Revenue Strategy until 2030, based on pan-European approaches. The purpose is to substantiate scientific and methodological approaches and practical proposals regarding the directions of transformation of the STS in the context of preparations for Ukraine’s acquisition of membership in the European Union, taking into account its role as the main tax instrument for supporting small businesses in the current institutional conditions. Methods. General scientific and special methods were used: analysis, synthesis, grouping, description, comparison, generalization, and abstract-logical. Results. The main approaches and methodological features of tax regulation and the application of tax incentives for small businesses in certain EU countries have been studied. The genesis of the regulatory framework for small business taxation has been examined. The conditions and prospects for harmonizing small business taxation in Ukraine with the corresponding practices in European Union countries have been revealed. Conclusions. It has been found that the main task of tax support for small businesses is to reduce administrative and financial burdens by applying reduced and fixed rates, lump-sum expenses, etc.; simplifying accounting procedures; applying a significant range of deductions from income aimed at ensuring social justice; applying a non-taxable minimum to entrepreneurs’ incomes, etc. The transformation of STS in our country should take into account the taxation practices in EU countries.
GLOBAL SOUTH’S JUDICIAL APPROACH TO PRESERVE THE ENVIRONMENT: AN OVERVIEW OF THE SELECTED SOUTH-ASIAN ENVIRONMENTAL JUDGMENTS
Authors: Farque Omar
Published: September 2024

Global South Asia’s domestic courts reportedly delivered many judgments innovating new practices and settling principles for environmental justice. The paper discusses the evolution of environmental law as a pertinent part of the human rights regime from a South Asian perspective and the role of the judiciary in promoting environmental justice. It highlights the region's diverse ecosystems and the increasing impact of climate change, emphasizing the importance of environmental litigation in enforcing environmental laws and protecting the region's natural resources. Hence this paper presents key case studies from Bangladesh, India, Pakistan, and Nepal, showcasing how the judiciary in these countries has upheld environmental principles and provided remedies for environmental harm.
Efektivitas Hidroterapi Dan Inhalasi Aroma Terapi Lavender Terhadap Intensitas Nyeri Pada Ibu Post Sectio Caesarea
Authors: Hernawati Erni Hernawati , TARWIYAH CHICI LAELATUL , Arianti Mirna
Published: September 2024

Trend persalinan sectio caesarea (SC) di Indoneisa sudah melebihi angka yangdirekomendasikan WHO. Efek samping yang dirasakan setelah SC dapat menyebabkanseorang ibu mengalami gannguan mobilisasi yang dapat mempengaruhi pemulihandan kesejahteraan ibu. Hidroterapi dan inhalasi aroma lavender telah dikenal luassebagai metode non-farmakologis untuk mengurangi rasa nyeri di negara-negara maju,penggunaan terapi ini masih terbatas di Indonesia dan negara-negara berkembang lainnya.Penelitian dan penerapan klinis kedua terapi ini dalam manajemen nyeri, khususnya padaibu post SC belum banyak dilakukan dan masih memerlukan lebih banyak bukti ilmiahuntuk mendukung efektivitasnya. Penelitian ini bertujuan untuk mengetahui efektivitaskombinasi hidroterapi dan inhalasi aroma terapi lavender terhadap penurunan intensitasnyeri pada ibu post SC. Desain penelitian ini menggunakan Quasy Eksperimen denganpendekatan pre-test pos-test with control group design. Adapun jumlah responden padapenelitian ini 60 ibu post SC yang memenuhi kriteria inklusi dan ekslusi yang dibagimenjadi 30 kelompok kontrol dan 30 kelompok intervensi menggunakan total sampling.Instrumen yang digunakan yaitu numeric rating scale kemudian di analisis menggunakanUji Wilcoxon. Penelitian ini dilakukan pada bulan Desember 2023 di Rumah Sakit MitraAnugrah Lestari. Hasil penelitian menunjukkan adanya penurunan intensitas nyeri setelahpemberian hidoterapi dan inhalasi aroma terapi lavender yaitu dari skala 6 (sedang)menjadi skala 3 (ringan) dengan p-value (0,000) ≤α (0,05) peneliti menyimpulkan adanyapengaruh hidroterapi dan inhalasi aromaterapi lavender terhadap intensitas nyeri pada ibupost SC.
O uso de marcadores moleculares como ferramenta no melhoramento genético de tambaqui (Colossoma Macropomum): uma revisão
Authors: Campos Eric Costa , Tsujii Karla Miky
Published: September 2024

O objetivo com o presente trabalho foi a elaboração de uma revisão que compilasse os principais pontos importantes sobre os marcadores microssatélites a variabilidade genética de Tambaqui (Colossoma macropomum), abordando os tópicos relativos ao cenário da aquicultura e à tecnología da reprodução de peixes nativos. O tambaqui é um dos peixes nativos mais consumidos no Brasil e, consequentemente um dos mais produzidos em cativeiro da mesma categoría. Para a produção em larga escala, conhecer os hábitos reprodutivos e estratégias de manejo se tornam uma parte esencial do proceso. O uso de microssatélites se apresenta como alternativa eficiente para os programas de melhoramento genético na piscicultura a nível mundial e, executar a técnica com peixes nativos se torna uma excelente prática, aumentando o desempenho de animais, visando a seleção de exemplares superiores com mais facilidade, proporcionando ao programa de melhoramento uma ferramenta robusta de produção de dados.
Combining network pharmacology, machine learning, molecular docking and molecular dynamic to explore the mechanism of Chufeng Qingpi decoction in treating schistosomiasis
Authors: Liu Minglu , Wang Yuxin , Deng Wen , Xie Jiahao , He Yanyao , Wang Liang , Zhang Jianbin , Cui Ming
Published: September 2024

Background

Although the Chufeng Qingpi Decoction (CQD) has demonstrated clinical effectiveness in the treatment of schistosomiasis, the precise active components and the underlying mechanisms of its therapeutic action remain elusive. To achieve a profound comprehension, we incorporate network pharmacology, bioinformatics analysis, molecular docking, and molecular dynamics simulations as investigative methodologies within our research framework.

Method

Utilizing TCMSP and UniProt, we identified formula components and targets. Cytoscape 3.10.0 was used to construct an herb–target interaction network. Genecards, DisGeNET, and OMIM databases were examined for disease-related objectives. A Venn diagram identified the intersection of compound and disease targets. Using Draw Venn, overlapping targets populated STRING for PPI network. CytoNCA identified schistosomiasis treatment targets. GO & KEGG enrichment analysis followed High-scoring genes in PPI were analyzed by LASSO, RF, SVM-RFE. Molecular docking & simulations investigated target-compound interactions.

Result

The component’s target network encompassed 379 nodes, 1629 edges, highlighting compounds such as wogonin, kaempferol, luteolin, and quercetin. Amongst the proteins within the PPI network, PTGS2, TNF, TGFB1, BCL2, TP53, IL10, JUN, MMP2, IL1B, and MYC stood out as the most prevalent entities. GO and KEGG revealed that mainly involved the responses to UV, positive regulation of cell migration and motility. The signal pathways encompassed Pathways in cancer, Lipid and atherosclerosis, Fluid shear stress and atherosclerosis, as well as the AGE-RAGE. Bioinformatics analysis indicated TP53 was the core gene. Ultimately, the molecular docking revealed that wogonin, kaempferol, luteolin, and quercetin each exhibited significant affinity in their respective interactions with TP53. Notably, kaempferol exhibited the lowest binding energy, indicating a highly stable interaction with TP53. Lastly, we validated the stability of the binding interaction between the four small molecules and the TP53 through molecular dynamics simulations. The molecular dynamics simulation further validated the strongest binding between TP53 and kaempferol. In essence, our research groundbreaking in its nature elucidates for the first time the underlying molecular mechanism of CQD in the therapeutic management of schistosomiasis, thereby providing valuable insights and guidance for the treatment of this disease.

Conclusion

This study uncovered the efficacious components and underlying molecular mechanisms of the Chufeng Qingpi Decoction in the management of schistosomiasis, thereby offering valuable insights for future fundamental research endeavors.
Efficacy of venoarterial extracorporeal membrane oxygenation with and without intra-aortic balloon pump in adult cardiogenic shock
Authors: Wang Haiwang , Li Chuanlong , Li Duo , Chen Yuansen , Li Wenli , Liu Yanqing , Li Yongnan , Fan Haojun , Hou Shike
Published: September 2024

Introduction

Intra-aortic balloon pump (IABP) is sometimes coupled with Venoarterial extracorporeal membrane oxygenation (VA-ECMO) to treat patients with cardiogenic shock. In this study, we attempted to evaluate the association of the IABP approach on survival and vascular complication rates in adults with cardiogenic shock undergoing VA-ECMO.

Methods

We performed a systematic search of original studies on VA-ECMO with and without IABP in PubMed, EMBASE, and the Cochrane Library.

Results

A total of 42 studies with 8,759 patients were included. The pooled in-hospital deaths of patients on VA-ECMO with and without IABP were 2,962/4,807 (61.61%) versus 2,666/3,952 (67.45%). VA-ECMO with IABP presents lower in-hospital mortality (risk ratio, 0.88; 95% CI, 0.86-0.91; P < 0.00001). In addition, IABP was associated with lower in-hospital mortality of patients with postcardiotomy cardiogenic shock and ischaemic heart disease. (risk ratio, 0.93; 95% CI, 0.87–0.98; P = 0.01; risk ratio, 0.85; 95% CI, 0.82–0.89; P < 0.00001). There was no significant difference in in-hospital morbidity in neurological, gastrointestinal, limb-related, bleeding, and infection complications between patients on VA-ECMO with and without IABP.

Discussion

In these observational studies, concomitant use of IABP and VA-ECMO in adult patients with cardiogenic shock was associated with reduced in-hospital mortality.

Systematic Review Registration

PROSPERO [CRD42017069259].
Non-functional alpha-cell hyperplasia with glucagon-producing NET: a case report
Authors: Cidade-Rodrigues Catarina , Santos Ana Paula , Calheiros Raquel , Santos Sara , Matos Catarina , Moreira Ana Paula , Inácio Isabel , Souteiro Pedro , Oliveira Joana , Jácome Manuel , Pereira Sofia S. , Henrique Rui , Torres Isabel , Monteiro Mariana P.
Published: September 2024

Introduction

Alpha-cell hyperplasia (ACH) is a rare pancreatic endocrine condition. Three types of ACH have been described: functional or nonglucagonoma hyperglucagonemic glucagonoma syndrome, reactive or secondary to defective glucagon signaling, and non-functional. Few cases of ACH with concomitant pancreatic neuroendocrine tumors (pNETs) have been reported and its etiology remains poorly understood. A case report of non-functional ACH with glucagon-producing NET is herein presented.

Case report

A 72-year-old male was referred to our institution for a 2 cm single pNET incidentally found during imaging for acute cholecystitis. The patient’s past medical history included type 2 diabetes (T2D) diagnosed 12 years earlier, for which he was prescribed metformin, dapagliflozin, and semaglutide. The pNET was clinically and biochemically non-functioning, apart from mildly elevated glucagon 217 pg/ml (<209), and 68Ga-SSTR PET/CT positive uptake was only found at the pancreatic tail (SUVmax 11.45). The patient underwent a caudal pancreatectomy and the post-operative 68Ga-SSTR PET/CT was negative. A multifocal well-differentiated NET G1, pT1N0M0R0 (mf) strongly staining for glucagon on a background neuroendocrine alpha-cell hyperplasia with some degree of acinar fibrosis was identified on pathology analysis.

Discussion and conclusion

This case reports the incidental finding of a clinically non-functioning pNET in a patient with T2D and elevated glucagon levels, unexpectedly diagnosed as glucagon-producing NET and ACH. A high level of suspicion was required to conduct the glucagon immunostaining, which is not part of the pathology routine for a clinically non-functioning pNET, and was key for the diagnosis that otherwise would have been missed. This case highlights the need to consider the diagnosis of glucagon-producing pNET on an ACH background even in the absence of glucagonoma syndrome.
Survival prediction in peritoneal mesothelioma: a nomogram based on SEER data and a Chinese cohort
Authors: Fang Yuting , Xiang Midan , Jiang Zhichao , Li Hongrui , Yuan Guangwen , Pei Wei , Li Wenbin , Sun Yongkun
Published: September 2024

Objective

This study aimed to develop nomogram predicting overall survival (OS) of patients with peritoneal mesothelioma (PeM) using data from Surveillance, Epidemiology, and End Results (SEER) database and a Chinese institution.

Methods

1,177 PeM patients from the SEER database were randomized into training and internal validation cohorts at a 7:3 ratio. An external validation cohort consisting of 109 patients was enrolled from a Chinese institution. Nomogram was constructed based on variables identified through multivariate Cox regression analysis and evaluated by consistency indices (C-index), calibration plots, and receiver operating characteristic (ROC) curves. Patients were stratified into different risk categories, and Kaplan-Meier survival analysis was used to assess OS differences among these groups.

Results

The nomogram, incorporating age, gender, histological type, T stage, M stage, and surgical status, demonstrated strong predictive capability with C-index values of 0.669 for the training cohort, 0.668 for the internal validation cohort, and 0.646 for the external validation cohort. The nomogram effectively stratified patients into high-risk and low-risk groups, with the high-risk group exhibiting significantly poorer OS (P < 0.05). Multivariate analysis confirmed gender, age, surgical intervention, and M stage as independent prognostic factors (P < 0.05). Specifically, male gender, older age, and unspecified M stage were linked to worse outcomes, while surgical intervention was associated with improved survival.

Conclusion

The nomogram provide a reliable tool for predicting the survival in PeM patients, facilitating more informed treatment decisions. Key independent prognostic factors include gender, age, surgical intervention, and M stage.
Discovery of ferroptosis-related genes in renal ischemia reperfusion and evaluate the potential impact on kidney transplantation
Authors: Zhou Yao , Yang Yuwei , Wang Bo , Chen Wan , Wei Yanlin , Wu Ruihua , Meng LingZhang , Lyu Liwen
Published: September 2024

Background

Renal ischemia reperfusion injury (IRI) is one of the pivotal event of acute kidney injury (AKI), and they are unavoidable in the process of kidney transplantation, which eventually leads to the loss of renal allograft. Ferroptosis is a newly identified programmed cell death. Recent studies have suggested that ferroptosis may participate in the pathophysiological process of renal IRI. Therefore, we aimed to determine biomarkers associated with ferroptosis during renal IRI and their impact on renal allografts.

Methods

We conducted a comprehensive bioinformatics analysis and established an IRI-AKI animal model to illustrate the critical role of ferroptosis-related hub genes (FRHGs) in IRI-AKI and their potential impact on kidney transplantation.

Results

In this study, we identified 60 ferroptosis-related genes (FRGs) in renal IRI based on the GSE148420 dataset and FerrDb database. And then we performed functional annotation analysis using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment. Protein-protein interaction (PPI) network was constructed by online tool String. EZH2, CDKN1A, PPARA, EGR1, ATF3, and CD44 were identited as six ferroptosis-related hubgenes (FRHGs) using four methods, including MMC, Degree, DMNC, and EPC. FRHGs expression level were verified by the validation sets GSE58438 and GSE126805. Protein expression level of FRHGs verified by Proteomics and Western blot. Cibersort was utilized to analyze immune cell infiltration during renal IRI as well as the correlation between FRHGs and immune cells. The GSE21374 dataset was used for renal allografts survival analysis. Finally, We induced the IRI-AKI animal model and illustrated the importance of FRGHs CD44 in ferroptosis and the accumulation of macrophages.

Conclusion

We identified 6 FRHGs. We found that FRHGs not only exhibited significant correlation with immune cells but also directly influenced the survival of transplanted kidneys in the human population. Among six FRHGs, only CD44 was overexpressed at both the gene and protein levels. Anti-CD44 exerts a protective effect by inhibiting ferroptosis and the accumulation of M1 macrophages during renal IRI. This study provided new insights into the pathogenesis of renal IRI and provided new evidence for its treatment.
Computational recognition of regulator genes and signature for ferroptosis with implications on immunological properties and clinical management of atopic dermatitis
Authors: Xu Lei , Guo Wenjuan , Hao Huirong , Yuan Jinping , Bai Bingxue
Published: September 2024

Background

Atopic dermatitis (AD) is a common chronic dermatitis of autoimmune origin that considerably affects the quality of life of patients. Ferroptosis, a newly regulated form of cell death, is essential for inflammation-related damage-associated molecular patterns (DAMPs). In this study, we aimed to identify ferroptosis regulators relevant to AD pathogenesis and reveal the mechanisms by which ferroptosis regulates the pathogenesis of AD.

Methods

We analyzed the GEO AD cohorts (GSE16161, GSE32924, GSE107361, and GSE120721), identifying AD-related differentially expressed genes (DEGs) using edgeR. Co-expression and STRING database analyses were used to elucidate the interactions between DEGs and ferroptosis markers. Through functional enrichment analysis, we defined potential biological functions within the protein-protein interaction (PPI) network and developed FerrSig using LASSO regression. The utility of FerrSig in guiding the clinical management of AD was evaluated using the GSE32473 cohort. Subsequently, our in silico findings were confirmed, and mechanistic insights were expanded through both in vitro and in vivo studies, validating the relevance of FerrSig.

Results

In the GEO AD cohort, 278 DEGs were identified, including seven ferroptosis signature genes. Co-expression analysis and STRING database review revealed a 63-node PPI network linked to cell cycle and pro-inflammatory pathways. Four ferroptosis genes (ALOXE3, FABP4, MAP3K14, and EGR1) were selected to create FerrSig, which was significantly downregulated in samples collected from patients with AD. In addition, immune-related signaling pathways were significantly differentially enriched between the stratifications of samples collected from patients with AD with high and low ferritin levels, whereas in the GSE32473 cohort, FerrSig was significantly increased in cohorts effectively treated with pimecrolimus or betamethasone. Finally, in vitro and in vivo models showed a notable FerrSig decrease in patients with AD versus healthy control. Treatment with betamethasone and tacrolimus restored FerrSig, and the magnitude of the increase in FerrSig was higher in samples collected from patients with AD with better efficacy assessments. In addition, FerrSig was significantly positively correlated with the ferroptosis inhibitors GPX4 and SLC7A11 and negatively correlated with reactive oxygen species (ROS) levels and p-STAT3/STAT3. This implies that the FerrSig signature genes may regulate ferroptosis through the JAK/STAT3 signaling pathway.

Conclusion

Our study further explored the pathogenesis of AD, and FerrSig could serve as a potential biomarker for identifying AD morbidity risks and determining treatment efficacy.
Nomogram using human epididymis protein 4 predicted concurrent endometrial cancer from endometrial atypical hyperplasia before surgery
Authors: Lou Yaochen , Jiang Feng , Du Yan , Guan Jun
Published: September 2024

Objective

To establish a nomogram based on presurgical predictors of concurrent endometrial cancer (EC) for patients diagnosed with endometrial atypical hyperplasia before definitive surgery (preoperative-EAH) to improve the risk stratification and clinical application.

Methods

Preoperative-EAH patients who underwent hysterectomy in a tertiary hospital from January 2020 to December 2022 were retrospectively analyzed. Independent predictors from the multivariate logistic regression model were used to establish a nomogram, and bootstrap resampling was used for internal validation.

Results

Of 370 preoperative-EAH patients, 23.4% were diagnosed with EC after definitive surgery (final-EC). Multivariate analyses found three independent predictors of final EC: human epididymis protein 4 (HE4) ≥43.50 pmol/L [odds ratio (OR) = 3.70; 95% confidence intervals (CI) = 2.06–6.67], body mass index (BMI) ≥ 28 kg/m2 (OR = 2.05; 95% CI = 1.14–3.69), and postmenopausal status, particularly at postmenopausal time ≥5 years (OR = 5.84, 95% CI = 2.51–13.55), which were used to establish a nomogram model. The bootstrap-corrected C-index of the nomogram was 0.733 (95% CI = 0.68–0.79), which was significantly higher than that of each individual factor. The calibration curve and decision curve showed good consistency and clinical net benefit of the model. At the maximum Youden index, 49.4% (43/87) of women in the high-risk group defined by nomogram had concurrent EC, versus 16.6% in the low-risk group (P< 0.001).

Conclusion

The nomogram based on HE4, menopausal status, and BMI was found with an improved predictive value to stratify preoperative-EAH patients at high risk of concurrent EC for better clinical management.
Topical treatment of chemotherapy-induced peripheral neuropathy (CIPN) with high-concentration (179 mg) capsaicin patch in breast cancer patients – results of the QUCIP study
Authors: Lux Michael Patrick , Flöther Lilit , Frömter Catrin , Rack Brigitte , Veselinovic Kristina , Heine Myriam , Paepke Stefan , Krabisch Petra , Quandel Tamara , Sabatowski Rainer
Published: September 2024

Background

Chemotherapy-induced peripheral neuropathy (CIPN) following oral or intravenous chemotherapy often results in neuropathic pain, accompanied by symptoms such tingling, burning and hypersensitivity to stimuli, with a notable decline in quality of life (QoL). Effective therapies for CIPN are lacking, with a high demand for analgesics to address this issue. The QUCIP study aimed to assess the effectiveness of high concentration (179 mg) capsaicin patch (HCCP) in alleviating neuropathic pain and associated symptoms in breast cancer patients with confirmed CIPN.

Methods

QUCIP is a prospective, multi-center observational study spanning 36 weeks with up to three HCCP treatments. Initial treatment (visit V0) was followed by two telephone contacts (T1, T2) and subsequent face-to-face visits every 12 weeks or upon retreatment (visits V1–V3). 73 female patients with painful CIPN post neoadjuvant/adjuvant breast cancer therapy were enrolled. Primary endpoint was the reduction of neuropathic pain symptom score (painDETECT®). Secondary endpoints included improvements in CIPN-specific QoL (QLQ-CIPN20), reductions in pain intensity (numeric pain rating scale, NPRS), and achievement of ≥ 30% and ≥ 50% pain reduction.

Results

Median age was 61 years, with 52.0% of patients experiencing peripheral neuropathic pain for > 1 year (> 2 years: 34.2%). The painDETECT® score significantly decreased from baseline (19.71 ± 4.69) to 15.80 ± 6.20 after initial treatment (p < 0.0001) and continued to decrease at follow-up visits. The NPRS indicated significant pain intensity reduction at each time point, particularly pronounced in patients receiving three HCCP treatments. Clinically significant pain relief of ≥ 30% increased from 25.0% at week 4 (T2) to 36.2%, 43.5%, and 40.0% at weeks 12 (V1), 24 (V2), and 36 (V3), respectively. The percentage of patients achieving pain relief of ≥ 50% increased from 14.7% at T2 to 15.5%, 21.7% and 32.5% at V1, V2 and V3, respectively. Patients further reported a significant improvement in their CIPN-related QoL throughout the study. Adverse drug reactions (ADRs) mainly included application site reactions.

Conclusion

In this study, HCCP shows benefit in managing CIPN in real-world settings. The data demonstrate a sustained and progressive reduction in neuropathic pain and symptomatology, confirming the clinical benefit of repeated treatment observed in former clinical trials. HCCP treatment has also the potential to significantly improve the QoL associated with CIPN. The safety profile of HCCP was confirmed, supporting its use in clinical practice.