bluuebunny/crossref_metadata_2025 huggingface dataset:
Transient Existence of Circulating Mesenchymal Stem Cells in the Deep Veins in Humans Following Long Bone Intramedullary Reaming
Authors: Churchman Sarah M , Jones Elena A , Roshdy Tarek , Cox George , Boxall Sally A , McGonagle Dennis , Giannoudis Peter V
Published: April 2020

The biology of mesenchymal stem cells (MSCs) in humans is incompletely understood and a possible role of systemically circulating cells in health and autoimmune disease remains controversial. Physiological movement of bone marrow MSCs to sites of injury would support the rationale for intravenous administration for relocation to damaged organs. We hypothesized that biophysical skeletal trauma rather than molecular cues may explain reported MSC circulation phenomena. Deep-femoral vein (FV) and matched peripheral vein blood samples (PVBs) were collected from patients undergoing lower-limb orthopaedic procedures during surgery (tibia using conventional sequential reaming, n = 9, femur using reamer/irrigator/aspirator (RIA), n = 15). PVBs were also taken from early (n = 15) and established (n = 12) rheumatoid arthritis (RA) patients and healthy donors (n = 12). Colony-forming unit-fibroblasts (CFU-Fs) were found in 17/36 FVBs but only 7/74 PVBs (mostly from femoral RIA); highly proliferative clonogenic cells were not generated. Only one colony was found in control/RA samples (n = 28). The rare CFU-Fs’ MSC nature was confirmed by phenotypic: CD105+/CD73+/CD90+ and CD19−/CD31−/CD33−/CD34−/CD45−/CD61−, and molecular profiles with 39/80 genes (including osteo-, chondro-, adipo-genic and immaturity markers) similar across multiple MSC tissue controls, but not dermal fibroblasts. Analysis of FVB-MSCs suggested that their likely origin was bone marrow as only two differences were observed between FVB-MSCs and IC-BM-MSCs (ACVR2A, p = 0.032 and MSX1, p = 0.003). Stromal cells with the phenotype and molecular profile of MSCs were scarcely found in the circulation, supporting the hypothesis that their very rare presence is likely linked to biophysical micro-damage caused by skeletal trauma (here orthopaedic manipulation) rather than specific molecular cues to a circulatory pool of MSCs capable of repair of remote organs or tissues. These findings support the use of organ resident cells or MSCs placed in situ to repair tissues rather than systemic administration.
The Influence of Preoperative Physical Activity on Postoperative Outcomes of Knee and Hip Arthroplasty Surgery in the Elderly: A Systematic Review
Authors: Vasta Sebastiano , Papalia Rocco , Torre Guglielmo , Vorini Ferruccio , Papalia Giuseppe , Zampogna Biagio , Fossati Chiara , Bravi Marco , Campi Stefano , Denaro Vincenzo
Published: April 2020

Total hip arthroplasty (THA) and total knee arthroplasty (TKA) represent two of the most common procedures in orthopedic surgery. The growing need to avoid physical impairment in elderly patients undergoing this kind of surgery puts the focus on the possibility to undertake a preoperative physical activity program to improve their fit and physical health at the time of surgery. A systematic review has been carried out with online databases including PubMed-Medline, Cochrane Central and Google Scholar. The aim was to retrieve available evidence concerning preoperative physical activity and exercise, before total knee or total hip arthroplasty in patients older than 65 years, and to clarify the role of this practice in improving postoperative outcomes. Results of the present systematic analysis showed that, for TKA, most of the studies demonstrated a comparable trend of postoperative improvement of Visual Analogue Scale (VAS), range of movement (ROM) and functional scores, and those of quality of life. There is insufficient evidence in the literature to draw final conclusions on the topic. Prehabilitation for patients undergoing TKA leads to shorter length of stay but not to an enhanced postoperative recovery. Concerning THA, although currently available data showed better outcomes in patients who underwent prehabilitation programs, there is a lack of robust evidence with appropriate methodology.
Deriving Immune Modulating Drugs from Viruses—A New Class of Biologics
Authors: Yaron Jordan R. , Zhang Liqiang , Guo Qiuyun , Burgin Michelle , Schutz Lauren N. , Awo Enkidia , Wise Lyn , Krause Kurt L. , Ildefonso Cristhian J. , Kwiecien Jacek M. , Juby Michael , Rahman Masmudur M. , Chen Hao , Moyer Richard W. , Alcami Antonio , McFadden Grant , Lucas Alexandra R.
Published: April 2020

Viruses are widely used as a platform for the production of therapeutics. Vaccines containing live, dead and components of viruses, gene therapy vectors and oncolytic viruses are key examples of clinically-approved therapeutic uses for viruses. Despite this, the use of virus-derived proteins as natural sources for immune modulators remains in the early stages of development. Viruses have evolved complex, highly effective approaches for immune evasion. Originally developed for protection against host immune responses, viral immune-modulating proteins are extraordinarily potent, often functioning at picomolar concentrations. These complex viral intracellular parasites have “performed the R&D”, developing highly effective immune evasive strategies over millions of years. These proteins provide a new and natural source for immune-modulating therapeutics, similar in many ways to penicillin being developed from mold or streptokinase from bacteria. Virus-derived serine proteinase inhibitors (serpins), chemokine modulating proteins, complement control, inflammasome inhibition, growth factors (e.g., viral vascular endothelial growth factor) and cytokine mimics (e.g., viral interleukin 10) and/or inhibitors (e.g., tumor necrosis factor) have now been identified that target central immunological response pathways. We review here current development of virus-derived immune-modulating biologics with efficacy demonstrated in pre-clinical or clinical studies, focusing on pox and herpesviruses-derived immune-modulating therapeutics.
The Outcome of Distal Radius Fractures with Concomitant Injuries Is Similar to those of Isolated Distal Radius Fractures Provided that an Arthroscopically Supported Treatment Is Performed
Authors: von Matthey Francesca , Schmid Karola , Zyskowski Michael , Deiler Stephan , Biberthaler Peter , Vester Helen
Published: April 2020

Background: Concomitant injuries of distal radius fractures (DRF) can have a fatal impact on the patients’ outcome. However, wrist arthroscopy is a costly and complex procedure. It remains elusive whether patients benefit from an additional arthroscopy. Methods: Patients with a DRF who were treated arthroscopically were enrolled. Fifty-six wrists were evaluated regarding their function by self-assessment with the Munich Wrist Questionnaire (MWQ). Thirty-nine patients were examined for postoperative strength and motion. Concomitant injuries were detected. Results: A total of 75% of the DRF were type C injuries (AO classification). Twenty-four cases (43%) were triangular fibrocartilaginous complex (TFCC) lesion, eight cases (14%) of scapholunate ligament (SL) injuries and seven cases (12%) were a combination of TFCC and SL ligament lesion. No difference in function could be detected between DRF with surgically addressed concomitant lesions and isolated DRF. Dorsalextension, palmarflexion and grip strength were significantly reduced in patients with DRF and concomitant injuries compared to the healthy wrist. However, patients with DRF and arthroscopically treated concomitant injuries had similar results to those suffering only from an isolated DRF. Conclusion: The increased occurrence of concomitant injuries is to be expected in intraarticular DRF. Patients with concomitant injuries benefit from an arthroscopically assisted fracture treatment and show similar results compared to isolated DRF.
Interhemispheric Functional Connectivity in the Primary Motor Cortex Assessed by Resting-State Functional Magnetic Resonance Imaging Aids Long-Term Recovery Prediction among Subacute Stroke Patients with Severe Hand Weakness
Authors: Min Yu-Sun , Park Jang Woo , Park Eunhee , Kim Ae-Ryoung , Cha Hyunsil , Gwak Dae-Won , Jung Seung-Hwan , Chang Yongmin , Jung Tae-Du
Published: April 2020

This study aimed to evaluate the usefulness of interhemispheric functional connectivity (FC) as a predictor of motor recovery in severe hand impairment and to determine the cutoff FC level as a clinically useful parameter. Patients with stroke (n = 22; age, 59.9 ± 13.7 years) who presented with unilateral severe upper-limb paresis and were confirmed to elicit no motor-evoked potential responses were selected. FC was measured using resting-state functional magnetic resonance imaging (rsfMRI) scans at 1 month from stroke onset. The good recovery group showed a higher FC value than the poor recovery group (p = 0.034). In contrast, there was no statistical difference in FC value between the good recovery and healthy control groups (p = 0.182). Additionally, the healthy control group showed a higher FC value than that shown by the poor recovery group (p = 0.0002). Good and poor recovery were determined based on Brunnstrom stage of upper-limb function at 6 months as the standard, and receiver operating characteristic curve indicated that a cutoff score of 0.013 had the greatest prognostic ability. In conclusion, interhemispheric FC measurement using rsfMRI scans may provide useful clinical information for predicting hand motor recovery during stroke rehabilitation.
A Longitudinal Study of the Antioxidant Barrier and Oxidative Stress in Morbidly Obese Patients after Bariatric Surgery. Does the Metabolic Syndrome Affect the Redox Homeostasis of Obese People?
Authors: Choromańska Barbara , Myśliwiec Piotr , Łuba Magdalena , Wojskowicz Piotr , Dadan Jacek , Myśliwiec Hanna , Choromańska Katarzyna , Zalewska Anna , Maciejczyk Mateusz
Published: April 2020

This is the first study to evaluate both the antioxidant barrier, glutathione metabolism, and oxidative damage to proteins and lipids in morbidly obese patients undergoing bariatric treatment. The study included 65 patients with class 3 obesity divided into two subgroups: morbidly obese patients without metabolic syndrome (OB) and obese patients with metabolic syndrome (OB + MS). Blood samples were collected before surgery as well as one, three, six, and twelve months after the bariatric treatment. Superoxide dismutase and reduced glutathione (GSH) were significantly decreased, whereas glutathione reductase and uric acid were enhanced in morbidly obese patients before bariatric surgery as compared to lean control. Moreover, in the OB group, we observed the increase of superoxide dismutase (SOD) and the decrease of uric acid (UA) after the bariatric treatment; however, these changes were not observed in the OB + MS group. The oxidative damage to proteins (advanced glycation end products, AGE; advanced oxidation protein products, AOPP) and lipids (8-isoprostanes, 8-isop; 4-hydroxynoneal) was higher in OB as well as OB + MS patients. We noticed that AGE and AOPP levels diminished after the bariatric treatment, whereas redox status (ratio of GSH to oxidized glutathione) was still reduced in the OB + MS group. Summarizing, morbid obesity is associated with disturbances in the antioxidant barrier and enhanced oxidative damage to proteins and lipids. Although bariatric surgery improves redox homeostasis in obese patients, those with metabolic syndrome show a continuous decrease in the antioxidant status. In patients undergoing bariatric treatment, antioxidant supplementation may be considered.
A Randomized Controlled Trial Comparing Novel Triple-Cuffed Double-Lumen Endobronchial Tubes with Conventional Double-Lumen Endobronchial Tubes for Lung Isolation
Authors: Kim Namo , Byon Hyo-Jin , Kim Go Eun , Park Chungon , Joe Young Eun , Suh Sung Min , Oh Young Jun
Published: April 2020

Placing a double-lumen endobronchial tube (DLT) in an appropriate position to facilitate lung isolation is essential for thoracic procedures. The novel ANKOR DLT is a DLT developed with three cuffs with a newly added carinal cuff designed to prevent further advancement by being blocked by the carina when the cuff is inflated. In this prospective study, the direction and depth of initial placement of ANKOR DLT were compared with those of conventional DLT. Patients undergoing thoracic surgery (n = 190) with one-lung ventilation (OLV) were randomly allocated into either left-sided conventional DLT group (n = 95) or left-sided ANKOR DLT group (n = 95). The direction and depth of DLT position were compared via fiberoptic bronchoscopy (FOB) after endobronchial intubation between the groups. There was no significant difference in the number of right mainstem endobronchial intubations between the two groups (p = 0.468). The difference between the initial depth of DLT placement and the target depth confirmed by FOB was significantly lower in the ANKOR DLT group than in the conventional DLT group (1.8 ± 1.8 vs. 12.9 ± 9.7 mm; p < 0.001). In conclusion, the ANKOR DLT facilitated its initial positioning at the optimal depth compared to the conventional DLT.
Quantitative Susceptibility Mapping and Vessel Wall Imaging as Screening Tools to Detect Microbleed in Sentinel Headache
Authors: Ishii Daizo , Nakagawa Daichi , Zanaty Mario , Roa Jorge A. , Al Kasab Sami , Shaban Amir , Hudson Joseph S. , Osorno-Cruz Carlos , Byer Stefano , Allan Lauren , Torner James C. , Awad Issam A. , Carroll Timothy J. , Samaniego Edgar A. , Hasan David M.
Published: April 2020

Background: MR-quantitative susceptibility mapping (QSM) can identify microbleeds (MBs) in intracranial aneurysm (IA) wall associated with sentinel headache (SH) preceding subarachnoid hemorrhage. However, its use is limited, due to associated skull base bonny and air artifact. MR-vessel wall imaging (VWI) is not limited by such artifact and therefore could be an alternative to QSM. The purpose of this study was to investigate the correlation between QSM and VWI in detecting MBs and to help develop a diagnostic strategy for SH. Methods: We performed a prospective study of subjects with one or more unruptured IAs in our hospital. All subjects underwent evaluation using 3T-MRI for MR angiography (MRA), QSM, and pre- and post-contrast VWI of the IAs. Presence/absence of MBs detected by QSM was correlated with aneurysm wall enhancement (AWE) on VWI. Results: A total of 40 subjects harboring 51 unruptured IAs were enrolled in the study. MBs evident on the QSM sequence was detected in 12 (23.5%) IAs of 11 subjects. All these subjects had a history of severe headache suggestive of SH. AWE was detected in 22 (43.1%) IAs. Using positive QSM as a surrogate for MBs, the sensitivity, specificity, positive predictive value, and negative predictive value of AWE on VWI for detecting MBs were 91.7%, 71.8%, 50%, and 96.6%, respectively. Conclusions: Positive QSM findings strongly suggested the presence of MBs with SH, whereas, the lack of AWE on VWI can rule it out with a probability of 96.6%. If proven in a larger cohort, combining QSM and VWI could be an adjunctive tool to help diagnose SH, especially in cases with negative or non-diagnostic CT and lumbar puncture.
Master Regulator Analysis of the SARS-CoV-2/Human Interactome
Authors: Guzzi Pietro H. , Mercatelli Daniele , Ceraolo Carmine , Giorgi Federico M.
Published: April 2020

The recent epidemic outbreak of a novel human coronavirus called SARS-CoV-2 causing the respiratory tract disease COVID-19 has reached worldwide resonance and a global effort is being undertaken to characterize the molecular features and evolutionary origins of this virus. In this paper, we set out to shed light on the SARS-CoV-2/host receptor recognition, a crucial factor for successful virus infection. Based on the current knowledge of the interactome between SARS-CoV-2 and host cell proteins, we performed Master Regulator Analysis to detect which parts of the human interactome are most affected by the infection. We detected, amongst others, affected apoptotic and mitochondrial mechanisms, and a downregulation of the ACE2 protein receptor, notions that can be used to develop specific therapies against this new virus.
Implementing Supported Self-Management in Community-Based Stroke Care: A Secondary Analysis of Nurses’ Perspectives
Authors: Kidd Lisa , Booth Joanne , Lawrence Maggie , Rowat Anne
Published: April 2020

The provision of supported self-management (SSM) is recommended in contemporary guidelines to address the longer-term needs and outcomes of stroke survivors and their families, yet its implementation across stroke pathways has been inconsistent. This paper presents a secondary analysis of qualitative data, which aims to identify and offer insight into the challenges of implementing SSM from the perspectives of community stroke nurses (n = 14). The findings revealed that the implementation of SSM in stroke is influenced by factors operating at multiple levels of the healthcare system. Contextual challenges arise because of different understandings and interpretations of what SSM is, what it comprises and professionals’ perceptions of their roles in its implementation in practice. A professionally controlled, one-size-fits-all model of SSM continues to be reinforced within organizations, offering few opportunities for nurses to deliver contextually tailored and person-centred SSM. In conclusion, there are many professional concerns and organizational tensions that need to be addressed across multiple layers of the healthcare system to achieve the consistent implementation of contextually tailored and person-centred SSM following a stroke. Attempts to address these challenges will help to narrow the gap between policy and practice of implementing SSM, ensuring that stroke survivors and families benefit from SSM in the longer-term.
Prevalence of Undiagnosed Obstructive Sleep Apnea Among Patients Hospitalized for Cardiovascular Disease and Associated In-Hospital Outcomes: A Scoping Review
Authors: Suen Colin , Wong Jean , Ryan Clodagh M. , Goh Samuel , Got Tiffany , Chaudhry Rabail , Lee Douglas S. , Chung Frances
Published: April 2020

Background: Obstructive sleep apnea (OSA) is associated with long-term cardiovascular morbidity and is highly prevalent in patients with cardiovascular disease (CVD). The objectives of this scoping review were to determine the prevalence of OSA inpatients hospitalized for CVD and to map the range of in-hospital outcomes associated with OSA. Methods: We searched MEDLINE(R), Embase, and Cochrane Databases for articles published from 1946–2018. We included studies involving non-surgical adults with OSA or at high risk of OSA who were hospitalized for CVD. The outcomes were considered as in-hospital if they were collected from admission up to 30 days post-discharge from hospital. Results: After the screening of 4642 articles, 26 studies were included for qualitative synthesis. Eligible studies included patients presenting with acute coronary syndromes (n = 19), congestive heart failure (n = 6), or any cardiovascular disease (n = 1). The pooled prevalence of OSA in cardiac inpatients was 48% (95% CI: 42–53). The in-hospital outcomes reported were mortality (n = 4), length of stay (n = 8), left ventricular ejection fraction (n = 8), peak troponin (n = 7), peak B-type natriuretic peptide (n = 4), and composite cardiovascular complications (n = 2). Conclusions: OSA is highly prevalent in the cardiac inpatient population. The outcomes reported included mortality, cardiac function, cardiac biomarkers, and resource utilization. There are significant knowledge gaps regarding the effect of treatment and OSA severity on these outcomes. The findings from this review serve to inform further areas of research on the management of OSA among patients with CVD.
The Multispecies Probiotic Effectively Reduces Homocysteine Concentration in Obese Women: A Randomized Double-Blind Placebo-Controlled Study
Authors: Majewska Karolina , Kręgielska-Narożna Matylda , Jakubowski Hieronim , Szulińska Monika , Bogdański Paweł
Published: April 2020

Dysregulated metabolism of homocysteine (Hcy) is associated with obesity. Supplementation with probiotics can potentially be a natural therapeutic method for metabolic disorders. The precise mechanism in which microbiota affect Hcy metabolism in obese individuals is still unknown. The aim of this study was to evaluate the effects of a 12-week supplementation with a multispecies probiotic on Hcy levels, oxidative stress, inflammation and lipid profile in obese patients. This randomized double-blind placebo-controlled trial was performed on 50 obese women (aged 45–70 years). Subjects were randomly assigned to take either a multispecies probiotic supplement (n = 25) or placebo (n = 25) for 12 weeks. The probiotic contained nine bacterial strains containing 2.5 × 109 CFU/g. Biochemical and anthropometric measurements were carried out at baseline and after 12 weeks of intervention. At the end of the study, a significant decrease in Hcy, tumor necrosis factor α (TNF-α), total cholesterol (TC), low-density lipoprotein cholesterol (LDL) and triglyceride (TG) levels were observed in the probiotic group. The amelioration of total antioxidant status (TAS) was also observed. The 12-week supplementation of the multispecies probiotic (Ecologic® BARIER) effectively reduced Hcy concentration, oxidative stress and inflammation, and improved the lipid profile. These multidirectional effects can potentially reduce cardiometabolic risks.
RAD51Bme Levels as a Potential Predictive Biomarker for PD-1 Blockade Response in Non-Small Cell Lung Cancer
Authors: Guerreiro Inês Maria , Barros-Silva Daniela , Lopes Paula , Cantante Mariana , Cunha Ana Luísa , Lobo João , Antunes Luís , Rodrigues Ana , Soares Marta , Henrique Rui , Jerónimo Carmen
Published: April 2020

Lung cancer (LC) cells frequently express high levels of programmed death-ligand 1 (PD-L1). Although these levels grossly correlate with the likelihood of response to specific checkpoint inhibitors, the response prediction is rather imperfect, and more accurate predictive biomarkers are mandatory. We examined the methylation profile of RAD51B (RAD51Bme) as a candidate predictive biomarker for anti-PD-1 therapy efficacy in non-small cell lung cancer (NSCLC), correlating with patients’ outcome. PD-L1 immunoexpression and RAD51Bme levels were analysed in NSCLC samples obtained from patients not treated with anti-PD-1 (Untreated Cohort (#1)) and patients treated with PD-1 blockade (Treated Cohort (#2)). Of a total of 127 patients assessed, 58.3% depicted PD-L1 positivity (PD-L1+). RAD51Bme levels were significantly associated with PD-L1 immunoexpression. Patients with PD-1 blockade clinical benefit disclosed higher RAD51Bme levels (p = 0.0390) and significantly lower risk of disease progression (HR 0.37; 95% CI: 0.15–0.88; p = 0.025). Combining RAD51Bme+ with PD-L1+ improved the sensitivity of the test to predict immunotherapy response. PD-L1+ was also associated with lower risk of death (HR 0.35; 95% CI: 0.15–0.81; p = 0.014). Thus, RAD51Bme levels might be combined with validated predictive biomarker PD-L1 immunostaining to select patients who will most likely experience clinical benefit from PD-1 blockade. The predictive value of RAD51Bme should be confirmed in prospective studies.
New Paradigms of Extended Thromboprophylaxis in Medically Ill Patients
Authors: MacDougall Kira , Spyropoulos Alex C
Published: April 2020

Extended thromboprophylaxis given to medically ill patients for up to 45 days following an acute hospitalization remains an emerging topic among many hospital-based health care providers. Recent advancements in the field of extended thromboprophylaxis using risk stratification and careful patient selection criteria have led to an improved safety profile of direct oral anticoagulants (DOACs) and established net clinical benefit when given to key patient subgroups at high risk of venous thromboembolism (VTE) and low risk of bleeding. The Food and Drug Administration (FDA) has now approved the DOACs betrixaban and rivaroxaban for both in-hospital and extended thromboprophylaxis in medically ill patients in these key subgroups, which represents more than one-quarter of hospitalized medically ill patients. This has potential to significantly reduce VTE-related morbidity and mortality for these patients. Emerging data also supports reductions in the risk of arterial thromboembolism in medically ill patients with extended thromboprophylaxis post-hospital discharge using DOACs. This article aims to review the most recent concepts of predicting and preventing VTE and to discuss emerging paradigms of extended thromboprophylaxis in hospitalized medically ill patients utilizing an individualized, risk-adapted approach.
Immunohistochemical Results of Soft Tissues around a New Implant Healing-Abutment Surface: A Human Study
Authors: Ghinassi Barbara , D’Addazio Gianmaria , Di Baldassarre Angela , Femminella Beatrice , Di Vincenzo Giorgio , Piattelli Maurizio , Gaggi Giulia , Sinjari Bruna
Published: April 2020

Although, the high success rate of implant rehabilitation treatment, the biological complications such as bone loss and peri-implantitis are still present. The creation of a coronal biological seal between the implant and the oral tissues seems to be a crucial point on preserving dental implants. The objective of this study was to immunohistochemically analyze the behavior of peri-implant soft tissues around a new implant healing-abutment surface on humans. A total of 30 soft tissue biopsies were collected after a healing period of 30 (±7) days, to analyze the expression of inflammatory (cluster of differentiation 63 (CD63), human neutrophil peptides 1–3 (HPN1–3)) and junctional (E-cadherin, occludin, and β-catenin) markers, on soft tissues around laser treated and machined alternated healing abutments. The evaluation demonstrated the whole area of the soft tissues adherent to the laser treated surface with a regular morphology. While several stress hallmarks in correspondence of machined surfaces were shown such as: (a) An irregular, disrupted, and discontinued basal membrane with an increased inflammation evident both the epithelial and connective tissues; (b) the absence or defective proper keratinization process of the external layer, and (c) damages in the cell to cell interaction. In conclusion, the laser treated surface is preferable to maintain the integrity and functionality of the gingiva epithelium.
Marine Sediments: Processes, Transport and Environmental Aspects
Authors: Di Risio Marcello , Hayes Donald F. , Pasquali Davide
Published: April 2020

In recent years, increasing attention has been paid to water quality and environmental aspects related to sediment transport driven by both ambient forcing and human activities [...]
Vegetation Structure, Species Composition, and Carbon Sink Potential of Urban Green Spaces in Nagpur City, India
Authors: Lahoti Shruti , Lahoti Ashish , Joshi Rajendra Kumar , Saito Osamu
Published: April 2020

Nagpur is rapidly urbanizing, and in the process witnessing decline in its green status which is one of the identities of the city. The study aims to understand the current species diversity, composition and structure in different classes of greens prevalent in the city. As urban green spaces (UGS) are also reservoirs of carbon stock, the study estimates their biomass. Through rigorous field work, data were collected from 246 sample plots across various UGS classes as pre-stratification. Then the biomass was estimated using non-destructive method with species-specific equation. The diversity of tree species recorded in UGS varies, with high diversity recorded in avenue plantation and institutional compounds. The overall variation in species composition among UGS classes was 36.8%. While in managed greens the species composition was similar, in institutional greens and forest it was different. Particularly, in forest the evenness was high with low diversity and low species richness. The structural distribution indicate lack of old trees in the city, with high number of tree species between diameter classes of 10–40 cm. Biomass was recorded high in road-side plantations (335 t ha−1) and playgrounds (324 t ha−1), and trees with bigger girth size where the main contributors. The dominant species indicates that high growth rate, tolerance to drought and pollution are the key attributes considered for species selection by local authorities. Though the city holds green image, vegetation along the avenues and institutions are stressed, exposed, and threatened by felling activities for grey infrastructure expansions. In such scenario, protection and preservation of older trees is crucial to maintain the carbon stock of the city. In addition, local authorities need to focus on effective afforestation programs through public participation to achieve high survival rate and reduce the maintenance cost. For species selection in addition to phenology and growth rate, tree biomass and life span needs to be considered to significantly enhance the urban environment and increase the benefits derived from UGS.
Large Scale Shrub Biomass Estimates for Multiple Purposes
Authors: Enes Teresa , Lousada José , Fonseca Teresa , Viana Hélder , Calvão Ana , Aranha José
Published: April 2020

With the increase of forest fires in Portugal in recent decades, a significant part of woodlands is being converted into shrubland areas. Background: From an ecological point of view, woodlands and shrublands play an essential role, as they not only prevent soil erosion and desertification, but also contribute to soil protection, habitat preservation and restoration, and also increased biodiversity for carbon sequestration. Concerning the shrublands, the assessment of their biomass is essential for evaluating the fuel load and forest fire behavior and also beneficial for obtaining estimates of carbon and biomass for energy use. Methods: In this study, we collected data about the potential shrub biomass accumulation along fifteen years in former burnt areas within North Portugal. Results: The achieved results showed that for a post-fire period ranging from one to 15 years, the accumulated shrubs’ biomass ranged from 0.12 up to 28.88 Mg ha−1. The model developed to estimate the shrub biomass using the time after a fire (age) as a predictor variable presented a high adjustment to data (p-value of the F statistic <0.01 and R2 = 0.89), allowing estimating shrub biomass regeneration within former burnt areas with an RMSE of 3.31 Mg ha−1. Conclusions: This paper provides practical information on the availability and assessment of shrub biomass in North Portugal, highlighting the suitability of shrubs as potential sources of biomass.
2,6-exo-8,12-exo-10-Butyl-13-oxa-3,5-dithia-10-azatetracyclo[5.5.1.02,6.08,12]tridecane-9,11-dione
Authors: Aitken R. , Fotherby Fiona , Slawin Alexandra
Published: April 2020

The title compound was obtained in low yield and spectroscopically characterised. Its X-ray structure was compared with the X-ray structures of other crystallographically-characterised 2-unsubstituted 1,3-dithiolanes.
Comparison between Plasma Electrolytic Oxidation Coating and Sandblasted Acid-Etched Surface Treatment: Histometric, Tomographic, and Expression Levels of Osteoclastogenic Factors in Osteoporotic Rats
Authors: Momesso Gustavo Antonio Correia , de Souza Santos Anderson Maikon , Fonseca e Santos João Matheus , da Cruz Nilson Cristino , Okamoto Roberta , Barão Valentim Adelino Ricardo , Siroma Rafael Shinoske , Shibli Jamil Awad , Perez Faverani Leonardo
Published: April 2020

Plasma electrolytic oxidation (PEO) has been a promising surface coating with better mechanical and antimicrobial parameters comparing to conventional treatment surfaces. This study evaluated the peri-implant bone repair using (PEO) surface coatings compared with sandblasted acid (SLA) treatment. For this purpose, 44 Wistar rats were ovariectomized (OVX-22 animals) or underwent simulated surgery (SS-22 animals) and received implants in the tibia with each of the surface coatings. The peri-implant bone subsequently underwent molecular, microstructural, bone turnover, and histometric analysis. Real-time PCR showed a higher expression of osteoprotegerin (OPG), receptor activator of nuclear kappa-B ligand (RANKL), and osteocalcin (OC) proteins in the SLA/OVX and PEO/SS groups (p < 0.05). Computed microtomography, confocal microscopy, and histometry showed similarity between the PEO and SLA surfaces, with a trend toward the superiority of PEO in OVX animals. Thus, PEO surfaces were shown to be promising for enhancing peri-implant bone repair in ovariectomized rats.
Characterisation of a High-Performance Al–Zn–Mg–Cu Alloy Designed for Wire Arc Additive Manufacturing
Authors: Morais Paulo J. , Gomes Bianca , Santos Pedro , Gomes Manuel , Gradinger Rudolf , Schnall Martin , Bozorgi Salar , Klein Thomas , Fleischhacker Dominik , Warczok Piotr , Falahati Ahmad , Kozeschnik Ernst
Published: April 2020

Ever-increasing demands of industrial manufacturing regarding mechanical properties require the development of novel alloys designed towards the respective manufacturing process. Here, we consider wire arc additive manufacturing. To this end, Al alloys with additions of Zn, Mg and Cu have been designed considering the requirements of good mechanical properties and limited hot cracking susceptibility. The samples were produced using the cold metal transfer pulse advanced (CMT-PADV) technique, known for its ability to produce lower porosity parts with smaller grain size. After material simulations to determine the optimal heat treatment, the samples were solution heat treated, quenched and aged to enhance their mechanical performance. Chemical analysis, mechanical properties and microstructure evolution were evaluated using optical light microscopy, scanning electron microscopy, energy dispersive X-ray spectroscopy, X-ray fluorescence analysis and X-ray radiography, as well as tensile, fatigue and hardness tests. The objective of this research was to evaluate in detail the mechanical properties and microstructure of the newly designed high-performance Al–Zn-based alloy before and after ageing heat treatment. The only defects found in the parts built under optimised conditions were small dispersed porosities, without any visible cracks or lack of fusion. Furthermore, the mechanical properties are superior to those of commercial 7xxx alloys and remarkably independent of the testing direction (parallel or perpendicular to the deposit beads). The presented analyses are very promising regarding additive manufacturing of high-strength aluminium alloys.
The Chitosan Implementation into Cotton and Polyester/Cotton Blend Fabrics
Authors: Flinčec Grgac Sandra , Tarbuk Anita , Dekanić Tihana , Sujka Witold , Draczyński Zbigniew
Published: April 2020

Chitosan is an environmentally friendly agent that is used to achieve the antimicrobial properties of textiles. Nowadays, the binding of chitosan to the textiles has been thoroughly researched due to the increasing demands on the stability of achieved properties during the textile care processes. Most crosslinking agents for chitosan are not safe for humans or environment, such as glutaric aldehyde (GA) and formaldehyde derivatives. Eco-friendly polycarboxyilic acids (PCAs) are usually used in after-treatment. In this work, chitosan powder was dissolved in citric acid with sodium hydrophosphite (SHP) as a catalyst. Standard cotton (CO) and polyester/cotton (PES/CO) fabrics were pretreated in 20% NaOH, similar to mercerization, in order to open the structure of the cotton fibers and hydrolyze polyester fibers, continued by finishing in the gelatin chitosan bath. Afterwards, the hot rinsing process, followed by drying and curing, closed the achieved structure. The main objective was to achieve durable antimicrobial properties to multiple maintenance cycles CO and PES/CO fabric in order to apply it in a hospital environment. The characterization of fabrics was performed after treatment, first and fifth washing cycles according ISO 6330:2012 by field emission scanning electron microscopy (FE-SEM), Fourier transform infrared spectroscopy (FTIR-ATR), electrokinetic analysis (EKA), by the determination of tensile properties and mechanical damage (wear), and the antimicrobial activity. The application of 20% NaOH led to the swelling and mercerization of cotton cellulose, and hydrolysis of polyester, resulting in better mechanical properties. It has been confirmed that the chitosan particles were well implemented into the cotton fiber and onto to the polyester component of PES/CO blend. The presence of chitosan was confirmed after five washing cycles, but in lower quantity. However, achieved antimicrobial activity is persistent.
Numerical Simulation of the Behavior of Hydrogen Source in a Novel Welding Process to Reduce Diffusible Hydrogen
Authors: Tashiro Shinichi , Mukai Naoki , Inoue Yoshihide , Murphy Anthony B. , Suga Tetsuo , Tanaka Manabu
Published: April 2020

This study aims to reduce the diffusible hydrogen content in deposited metal during gas metal arc welding (GMAW) and flux-cored arc welding (FCAW) which induces cold cracking. To achieve this, a novel welding torch with a dual gas nozzle has been developed. This special welding torch decreases the hydrogen source gas evaporated from a welding wire by the suction from the inner gas nozzle. In order to improve the suction efficiency of this evaporated gas, precise control of the suction gas flow is indispensable. In this paper, a simplified numerical simulation model of this process has been described. This model can take account of the evaporation of the hydrogen source gas from the wire while simulating the behavior of the shielding gas and the arc. Using this model, the effect of suction nozzle structure and torch operating conditions on suction gas flow pattern and suction efficiency was also investigated to understand the process mechanism. Furthermore, the diffusible hydrogen content in deposited metal was measured by chromatography as a validation step. Results show that some of the shielding gas introduced from a shielding nozzle was drawn inward and also branched into an upward flow that was sucked into the suction nozzle and a downward flow to a base metal. This branching height was defined as the suction limit height, which decisively governed the suction efficiency. As a result, in order to reduce the diffusible hydrogen, it was suggested that the suction limit height should be controlled towards below the wire position, where the evaporation rate of the hydrogen source gas peaks through optimization of the suction nozzle design and the torch operating conditions.
A Comparative Study of (Cd,Zn)S Buffer Layers for Cu(In,Ga)Se2 Solar Panels Fabricated by Chemical Bath and Surface Deposition Methods
Authors: Bae Dowon
Published: April 2020

Scale-up to large-area Cu(In,Ga)Se2 (CIGS) solar panels is proving to be much more complicated than expected. Particularly, the non-vacuum wet-chemical buffer layer formation step has remained a challenge and has acted as a bottleneck in industrial implementations for mass-production. This technical note deals with the comparative analysis of the impact on different methodologies for the buffer layer formation on CIGS solar panels. Cd(1-x)ZnxS ((Cd,Zn)S) thin films were prepared by chemical bath deposition (CBD), and chemical surface deposition (CSD) for 24-inch (37 cm × 47 cm) patterned CIGS solar panel applications. Buffer layers deposited by the CBD method showed a higher Zn addition level and transmittance than those prepared by the CSD technique due to the predominant cluster-by-cluster growth mechanism, and this induced a difference in the solar cell performance, consequently. The CIGS panels with (Cd,Zn)S buffer layer formed by the CBD method showed a 0.5% point higher conversion efficiency than that of panels with a conventional CdS buffer layer, owing to the increased current density and open-circuit voltage. The samples with the CSD (Cd,Zn)S buffer layer also increased the conversion efficiency with 0.3% point than conventional panels, but mainly due to the increased fill factor.
Material Analysis of Steel Fibre Reinforced High-Strength Concrete in Terms of Flexural Behaviour. Experimental and Numerical Investigation
Authors: Bywalski Czesław , Kaźmierowski Maciej , Kamiński Mieczysław , Drzazga Michał
Published: April 2020

The paper presents the results of tests for flexural tensile strength (fct,fl) and fracture energy (Gf) in a three-point bending test of prismatic beams with notches, which were made of steel fibre reinforced high-strength concrete (SFRHSC). The registration of the conventional force–displacement (F–δ) relationship and unconventional force-crack tip opening displacement (CTOD) relationship was made. On the basis of the obtained test results, estimations of parameters fct,fl and Gf in the function of fibre reinforcement ratio were carried out. The obtained results were applied to building and validating a numerical model with the use of the finite element method (FEM). A non-linear concrete damaged plasticity model CDP was used for the description of the concrete. The obtained FEM results were compared with the experimental ones that were based on the assumed criteria. The usefulness of the flexural tensile strength and fracture energy parameters for defining the linear form of weakening of the SFRHSC material under tension, was confirmed. Own equations for estimating the flexural tensile strength and fracture energy of SFRHSC, as well as for approximating deflections (δ) of SFRHSC beams as the function of crack tip opening displacement (CTOD) instead of crack mouth opening displacement (CMOD), were proposed.
Hybridized Love Waves in a Guiding Layer Supporting an Array of Plates with Decorative Endings
Authors: Pham Kim , Maurel Agnès , Félix Simon , Guenneau Sébastien
Published: April 2020

This study follows from Maurel et al., Phys. Rev. B 98, 134311 (2018), where we reported on direct numerical observations of out-of-plane shear surface waves propagating along an array of plates atop a guiding layer, as a model for a forest of trees. We derived closed form dispersion relations using the homogenization procedure and investigated the effect of heterogeneities at the top of the plates (the foliage of trees). Here, we extend the study to the derivation of a homogenized model accounting for heterogeneities at both endings of the plates. The derivation is presented in the time domain, which allows for an energetic analysis of the effective problem. The effect of these heterogeneous endings on the properties of the surface waves is inspected for hard heterogeneities. It is shown that top heterogeneities affect the resonances of the plates, hence modifying the cut-off frequencies of a wave mathematically similar to the so-called Spoof Plasmon Polariton (SPP) wave, while the bottom heterogeneities affect the behavior of the layer, hence modifying the dispersion relation of the Love waves. The complete system simply mixes these two ingredients, resulting in hybrid surface waves accurately described by our model.
In Situ Recrystallization of Mesoporous Carbon–Silica Composite for the Synthesis of Hierarchically Porous Zeolites
Authors: Du Jun , Wang Yan , Wang Yan , Li Ruifeng
Published: April 2020

Hierarchically porous ZSM-5 was prepared by utilizing a two-step crystallization procedure with carbon–silica composites as precursors. The hierarchically porous zeolites obtained a regular mesoporous structure with aluminum incorporated into the carbon–silica composite frameworks. The carbon–silica composite zeolites were characterized by XRD, TEM, SEM, and nitrogen adsorption/desorption. As-prepared hierarchical zeolites were used in the 1,3,5-triisopropylbenzene (TIPB) cracking reaction and exhibited significantly high TIPB conversion, while the accessibility factors were also determined.
Constructing Machine Tool Foundations Using an LMP Alloy
Authors: Zhang Yi , Chen Wanlu , Dou Suqin , Li Panpan , Gu Hai , Dong Ren-E
Published: April 2020

Currently, the construction of machine tool foundations is a complicated and lengthy procedure with a limited flexibility. In this paper, we present a novel system for constructing machine tool foundations that replaces the need for concrete or concrete-polymer hybrids with a low melting point (LMP) alloy. The system uses a hot bath method to maintain the LMP alloy grouting in liquid form. A fixing device is used to control the embedded depth and positional accuracy of the foundation bolt assembly. The grouting material is injected into the foundation pit by a filling device. This can be extracted from the foundation pit in a later stage with the aid of a recycling device, enabling new machine tool foundations to be manufactured by reusing the LMP alloy grouting material. A prototype was built to test the proposed design. The results show that the system can construct machine tool foundations in a single application, without the delays associated with concrete-based construction, lowering both the economic and environmental cost.
Classical Lagrange Interpolation Based on General Nodal Systems at Perturbed Roots of Unity
Authors: Berriochoa Elías , Cachafeiro Alicia , Castejón Alberto , García-Amor José Manuel
Published: April 2020

The aim of this paper is to study the Lagrange interpolation on the unit circle taking only into account the separation properties of the nodal points. The novelty of this paper is that we do not consider nodal systems connected with orthogonal or paraorthogonal polynomials, which is an interesting approach because in practical applications this connection may not exist. A detailed study of the properties satisfied by the nodal system and the corresponding nodal polynomial is presented. We obtain the relevant results of the convergence related to the process for continuous smooth functions as well as the rate of convergence. Analogous results for interpolation on the bounded interval are deduced and finally some numerical examples are presented.
Reduced Dilation-Erosion Perceptron for Binary Classification
Authors: Valle Marcos Eduardo
Published: April 2020

Dilation and erosion are two elementary operations from mathematical morphology, a non-linear lattice computing methodology widely used for image processing and analysis. The dilation-erosion perceptron (DEP) is a morphological neural network obtained by a convex combination of a dilation and an erosion followed by the application of a hard-limiter function for binary classification tasks. A DEP classifier can be trained using a convex-concave procedure along with the minimization of the hinge loss function. As a lattice computing model, the DEP classifier assumes the feature and class spaces are partially ordered sets. In many practical situations, however, there is no natural ordering for the feature patterns. Using concepts from multi-valued mathematical morphology, this paper introduces the reduced dilation-erosion (r-DEP) classifier. An r-DEP classifier is obtained by endowing the feature space with an appropriate reduced ordering. Such reduced ordering can be determined using two approaches: one based on an ensemble of support vector classifiers (SVCs) with different kernels and the other based on a bagging of similar SVCs trained using different samples of the training set. Using several binary classification datasets from the OpenML repository, the ensemble and bagging r-DEP classifiers yielded mean higher balanced accuracy scores than the linear, polynomial, and radial basis function (RBF) SVCs as well as their ensemble and a bagging of RBF SVCs.
Gene-Similarity Normalization in a Genetic Algorithm for the Maximum k-Coverage Problem
Authors: Yoon Yourim , Kim Yong-Hyuk
Published: April 2020

The maximum k-coverage problem (MKCP) is a generalized covering problem which can be solved by genetic algorithms, but their operation is impeded by redundancy in the representation of solutions to MKCP. We introduce a normalization step for candidate solutions based on distance between genes which ensures that a standard crossover such as uniform and n-point crossovers produces a feasible solution and improves the solution quality. We present results from experiments in which this normalization was applied to a single crossover operation, and also results for example MKCPs.