bluuebunny/crossref_metadata_2025 huggingface dataset:
Development and a Validation of a Charge Sensitive Organic Rankine Cycle (ORC) Simulation Tool
Authors: Ziviani Davide , Woodland Brandon , Georges Emeline , Groll Eckhard , Braun James , Horton W. , Van den Broek Martijn , De Paepe Michel
Published: May 2016

Despite the increasing interest in organic Rankine cycle (ORC) systems and the large number of cycle models proposed in the literature, charge-based ORC models are still almost absent. In this paper, a detailed overall ORC simulation model is presented based on two solution strategies: condenser subcooling and total working fluid charge of the system. The latter allows the subcooling level to be predicted rather than specified as an input. The overall cycle model is composed of independent models for pump, expander, line sets, liquid receiver and heat exchangers. Empirical and semi-empirical models are adopted for the pump and expander, respectively. A generalized steady-state moving boundary method is used to model the heat exchangers. The line sets and liquid receiver are used to better estimate the total charge of the system and pressure drops. Finally, the individual components are connected to form a cycle model in an object-oriented fashion. The solution algorithm includes a preconditioner to guess reasonable values for the evaporating and condensing temperatures and a main cycle solver loop which drives to zero a set of residuals to ensure the convergence of the solution. The model has been developed in the Python programming language. A thorough validation is then carried out against experimental data obtained from two test setups having different nominal size, working fluids and individual components: (i) a regenerative ORC with a 5 kW scroll expander and an oil flooding loop; (ii) a regenerative ORC with a 11 kW single-screw expander. The computer code is made available through open-source dissemination.
Exploring the Spatial Heterogeneity of Individual Preferences for Ambient Heating Systems
Authors: Franceschinis Cristiano , Scarpa Riccardo , Thiene Mara , Rose John , Moretto Michele , Cavalli Raffaele
Published: May 2016

The estimation and policy use of spatially explicit discrete choice models has yet to receive serious attention from practitioners. In this study we aim to analyze how geographical variables influence individuals’ sensitivity to key features of heating systems, namely investment cost and CO2 emissions. This is of particular policy interest as heating systems are strongly connected to two major current environmental issues: emissions of pollutants and increased use of renewable resources. We estimate a mixed logit model (MXL) to spatially characterize preference heterogeneity in the mountainous North East of Italy. Our results show that geographical variables are significant sources of variation of individual’s sensitivity to the investigated attributes of the system. We generate maps to show how the willingness to pay to avoid CO2 emissions varies across the region and to validate our estimates ex-post. We discuss why this could be a promising approach to inform applied policy decisions.
Preconceptional Iron Intake and Gestational Diabetes Mellitus
Authors: Darling Anne , Mitchell Allen , Werler Martha
Published: May 2016

Our objective was to assess the impact of preconceptional heme and non-heme iron on gestational diabetes mellitius (GDM) in the Boston University Slone Epidemiology Birth Defects Study (BDS). This retrospective cohort analysis included 7229 participants enrolled in the BDS between 1998 and 2008 who gave birth to non-malformed infants and were free of pre-existing diabetes. All data were collected through structured interviews conducted within 6 months of delivery. Calorie-adjusted and multivariable odds ratios (ORs) and 95% confidence intervals (CIs) were calculated using logistic regression models. Preconceptional dietary heme iron was modestly associated with an elevated risk of GDM among those (multivariable OR comparing the fifth quintile to the first: 1.55; 95% CI 0.98, 2.46). Conversely, preconceptional dietary non-heme iron was associated with a decreased risk of GDM among those in the fifth quintile of intake compared to the first (multivariable OR: 0.48; 95% CI 0.28, 0.81). Women who consumed supplemental iron during preconception also had a decreased risk of GDM (multivariable OR: 0.78; 95% CI 0.60, 1.02). In conclusion, our data support a positive association between preconceptional heme iron intake and GDM and an inverse association between preconceptional non-heme iron intake from foods and preconceptional intake from supplements.
Both Direct and Vicarious Experiences of Nature Affect Children’s Willingness to Conserve Biodiversity
Authors: Soga Masashi , Gaston Kevin , Yamaura Yuichi , Kurisu Kiyo , Hanaki Keisuke
Published: May 2016

Children are becoming less likely to have direct contact with nature. This ongoing loss of human interactions with nature, the extinction of experience, is viewed as one of the most fundamental obstacles to addressing global environmental challenges. However, the consequences for biodiversity conservation have been examined very little. Here, we conducted a questionnaire survey of elementary schoolchildren and investigated effects of the frequency of direct (participating in nature-based activities) and vicarious experiences of nature (reading books or watching TV programs about nature and talking about nature with parents or friends) on their affective attitudes (individuals’ emotional feelings) toward and willingness to conserve biodiversity. A total of 397 children participated in the surveys in Tokyo. Children’s affective attitudes and willingness to conserve biodiversity were positively associated with the frequency of both direct and vicarious experiences of nature. Path analysis showed that effects of direct and vicarious experiences on children’s willingness to conserve biodiversity were mediated by their affective attitudes. This study demonstrates that children who frequently experience nature are likely to develop greater emotional affinity to and support for protecting biodiversity. We suggest that children should be encouraged to experience nature and be provided with various types of these experiences.
Synthesis, Biological Activity, and Apoptotic Properties of NO-Donor/Enmein-Type ent-Kauranoid Hybrids
Authors: Li Dahong , Hu Xu , Han Tong , Xu Shengtao , Zhou Tingting , Wang Zhenzhong , Cheng Keguang , Li Zhanlin , Hua Huiming , Xiao Wei , Xu Jinyi
Published: May 2016

Herein, we reported on a series of synthetic nitric oxide-releasing enmein-type diterpenoid hybrids (9a–i). All the target compounds showed potent antibacterial activity against selected Gram-positive bacteria S. aureus and B. subtilis. The antiproliferative activity against human tumor K562, MGC-803, CaEs-17 and Bel-7402 cells, and human normal liver cells L-02 was tested and the structure activity relationships (SARs) were also concluded. Compounds 9b and 9d showed the best activity against S. aureus and B. subtilis with the same minimal inhibitory concentrations (MICs) of 4 and 2 μg/mL, respectively. The derivative 9f displayed IC50 values of 1.68, 1.11, 3.60 and 0.72 μM against the four cancer cell lines above and 18.80 μM against normal liver cells L-02; meanwhile, 9f also released a high level of NO at the time point of 60 min of 22.24 μmol/L. Furthermore, it was also found that 9f induced apoptosis via the mitochondria-related pathway and arrested cell cycle of Bel-7402 cells at S phase. These findings might be important to explore new chemical entities for the main causes of in-hospital mortality of S. aureus infection, combined with a solid tumor.
The Protective Effect of Grape-Seed Proanthocyanidin Extract on Oxidative Damage Induced by Zearalenone in Kunming Mice Liver
Authors: Long Miao , Yang Shu-Hua , Han Jian-Xin , Li Peng , Zhang Yi , Dong Shuang , Chen Xinliang , Guo Jiayi , Wang Jun , He Jian-Bin
Published: May 2016

Although grape-seed proanthocyanidin extract (GSPE) demonstrates strong anti-oxidant activity, little research has been done to clearly reveal the protective effects on the hepatotoxicity caused by zearalenone (ZEN). This study is to explore the protective effect of GSPE on ZEN-induced oxidative damage of liver in Kunming mice and the possible protective molecular mechanism of GSPE. The results indicated that GSPE could greatly reduce the ZEN-induced increase of serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activities. GSPE also significantly decreased the content of MDA but enhanced the activities of antioxidant enzymes SOD and GSH-Px. The analysis indicated that ZEN decreased both mRNA expression levels and protein expression levels of nuclear erythroid2-related factor2 (Nrf2). Nrf2 is considered to be an essential antioxidative transcription factor, as downstream GSH-Px, γ-glutamyl cysteine synthetase (γ-GCS), hemeoxygenase-1 (HO-1), and quinone oxidoreductase 1 (NQO1) decreased simultaneously, whereas the pre-administration of GSPE groups was shown to elevate these expressions. The results indicated that GSPE exerted a protective effect on ZEN-induced hepatic injury and the mechanism might be related to the activation of the Nrf2/ARE signaling pathway.
Crystal Structure of Cytochrome P450 (CYP105P2) from Streptomyces peucetius and Its Conformational Changes in Response to Substrate Binding
Authors: Lee Chang , Lee Joo-Ho , Rimal Hemraj , Park Hyun , Lee Jun , Oh Tae-Jin
Published: May 2016

Cytochrome P450 monooxygenases (CYP, EC 1.14.14.1) belong to a large family of enzymes that catalyze the hydroxylation of various substrates. Here, we present the crystal structure of CYP105P2 isolated from Streptomyces peucetius ATCC27952 at a 2.1 Å resolution. The structure shows the presence of a pseudo-ligand molecule in the active site, which was co-purified fortuitously and is presumed to be a biphenyl derivative. Comparison with previously determined substrate-bound CYP structures showed that binding of the ligand produces large and distinctive conformational changes in α2–α3, α7–α9, and the C-terminal loop regions. This structural flexibility confirms our previous observation that CYP105P2 can accommodate a broad range of ligands. The structure complexed with a pseudo-ligand provides the first molecular view of CYP105P2–ligand interactions, and it indicates the involvement of hydrophobic residues (Pro82, Ala181, Met187, Leu189, Leu193, and Ile236) in the interactions between hydrophobic ligands and CYP105P2. These results provide useful insights into the structural changes involved in the recognition of different ligands by CYP105P2.
Applications of Fourier Transform Ion Cyclotron Resonance (FT-ICR) and Orbitrap Based High Resolution Mass Spectrometry in Metabolomics and Lipidomics
Authors: Ghaste Manoj , Mistrik Robert , Shulaev Vladimir
Published: May 2016

Metabolomics, along with other “omics” approaches, is rapidly becoming one of the major approaches aimed at understanding the organization and dynamics of metabolic networks. Mass spectrometry is often a technique of choice for metabolomics studies due to its high sensitivity, reproducibility and wide dynamic range. High resolution mass spectrometry (HRMS) is a widely practiced technique in analytical and bioanalytical sciences. It offers exceptionally high resolution and the highest degree of structural confirmation. Many metabolomics studies have been conducted using HRMS over the past decade. In this review, we will explore the latest developments in Fourier transform mass spectrometry (FTMS) and Orbitrap based metabolomics technology, its advantages and drawbacks for using in metabolomics and lipidomics studies, and development of novel approaches for processing HRMS data.
Fabrication of Gold-Coated Ultra-Thin Anodic Porous Alumina Substrates for Augmented SERS
Authors: Toccafondi Chiara , Proietti Zaccaria Remo , Dante Silvia , Salerno Marco
Published: May 2016

Anodic porous alumina (APA) is a nanostructured material used as a template in several nanotechnological applications. We propose the use of APA in ultra-thin form (<100 nm) for augmented surface-enhanced Raman scattering (SERS). Here, the effect of in-depth thinning of the APA nanostructures for possible maximization of SERS was addressed. Anodization was carried out on ultra-thin films of aluminum on glass and/or silicon, followed by pore-opening. Gold (Au) was overcoated and micro‑Raman/SERS measurements were carried out on test target analytes. Finite integration technique simulations of the APA-Au substrate were used both for the experimental design and simulations. It was observed that, under optimized conditions of APA and Au thickness, the SERS enhancement is higher than on standard APA-Au substrates based on thin (~100 nm) APA by up to a factor of ~20 for test molecules of mercaptobenzoic acid. The agreement between model and experimental results confirms the current understanding of SERS as being mainly due to the physical origin of plasmon resonances. The reported results represent one step towards micro-technological, integrated, disposable, high-sensitivity SERS chemical sensors and biosensors based on similar substrates.
Bioactive and Antibacterial Glass Powders Doped with Copper by Ion-Exchange in Aqueous Solutions
Authors: Miola Marta , Verné Enrica
Published: May 2016

In this work, two bioactive glass powders (SBA2 and SBA3) were doped with Cu by means of the ion-exchange technique in aqueous solution. SBA2 glass was subjected to the ion-exchange process by using different Cu salts (copper(II) nitrate, chloride, acetate, and sulphate) and concentrations. Structural (X-ray diffraction-XRD), morphological (Scanning Electron Microscopy-SEM), and compositional (Energy Dispersion Spectrometry-EDS) analyses evidenced the formation of crystalline phases for glasses ion-exchanged in copper(II) nitrate and chloride solutions; while the ion-exchange in copper(II) acetate solutions lead to the incorporation of higher Cu amount than the ion-exchange in copper(II) sulphate solutions. For this reason, the antibacterial test (inhibition halo towards S. aureus) was performed on SBA2 powders ion-exchanged in copper(II) acetate solutions and evidenced a limited antibacterial effect. A second glass composition (SBA3) was developed to allow a greater incorporation of Cu in the glass surface; SBA3 powders were ion-exchanged in copper(II) acetate solutions (0.01 M and 0.05 M). Cu-doped SBA3 powders showed an amorphous structure; morphological analysis evidenced a rougher surface for Cu-doped powders in comparison to the undoped glass. EDS and X-ray photoelectron spectroscopy (XPS) confirmed the Cu introduction as Cu(II) ions. Bioactivity test in simulated body fluid (SBF) showed that Cu introduction did not alter the bioactive behaviour of the glass. Finally, inhibition halo test towards S. aureus evidenced a good antimicrobial effect for glass powders ion-exchanged in copper(II) acetate solutions 0.05 M.
Graphene–Gold Nanoparticles Hybrid—Synthesis, Functionalization, and Application in a Electrochemical and Surface-Enhanced Raman Scattering Biosensor
Authors: Khalil Ibrahim , Julkapli Nurhidayatullaili , Yehye Wageeh , Basirun Wan , Bhargava Suresh
Published: May 2016

Graphene is a single-atom-thick two-dimensional carbon nanosheet with outstanding chemical, electrical, material, optical, and physical properties due to its large surface area, high electron mobility, thermal conductivity, and stability. These extraordinary features of graphene make it a key component for different applications in the biosensing and imaging arena. However, the use of graphene alone is correlated with certain limitations, such as irreversible self-agglomerations, less colloidal stability, poor reliability/repeatability, and non-specificity. The addition of gold nanostructures (AuNS) with graphene produces the graphene–AuNS hybrid nanocomposite which minimizes the limitations as well as providing additional synergistic properties, that is, higher effective surface area, catalytic activity, electrical conductivity, water solubility, and biocompatibility. This review focuses on the fundamental features of graphene, the multidimensional synthesis, and multipurpose applications of graphene–Au nanocomposites. The paper highlights the graphene–gold nanoparticle (AuNP) as the platform substrate for the fabrication of electrochemical and surface-enhanced Raman scattering (SERS)-based biosensors in diverse applications as well as SERS-directed bio-imaging, which is considered as an emerging sector for monitoring stem cell differentiation, and detection and treatment of cancer.
A Comparative Study on Permanent Mold Cast and Powder Thixoforming 6061 Aluminum Alloy and Sicp/6061Al Composite: Microstructures and Mechanical Properties
Authors: Zhang Xuezheng , Chen Tijun , Qin He , Wang Chong
Published: May 2016

Microstructural and mechanical characterization of 10 vol% SiC particles (SiCp) reinforced 6061 Al-based composite fabricated by powder thixoforming (PTF) was investigated in comparison with the PTF and permanent mold cast (PMC) 6061 monolithic alloys. The results reveal that the microstructure of the PMC alloy consists of coarse and equiaxed α dendrites and interdendritic net-like eutectic phases. However, the microstructure of the PTF composite, similar to that of the PTF alloy, consists of near-spheroidal primary particles and intergranular secondarily solidified structures except SiCp, which are distributed in the secondarily solidified structures. The eutectics amount in the PTF materials is distinctly lower than that in the PMC alloy, and the microstructures of the former materials are quite compact while that of the latter alloy is porous. Therefore, the PTF alloy shows better tensile properties than the PMC alloy. Owing to the existence of the SiC reinforcing particles, the PTF composite attains an ultimate tensile strength and yield strength of 230 MPa and 128 MPa, representing an enhancement of 27.8% and 29.3% than those (180 MPa and 99 MPa) of the PTF alloy. A modified model based on three strengthening mechanisms was proposed to calculate the yield strength of the PTF composite. The obtained theoretical results were quite consistent with the experimental data.
A Discussion on the Interpretation of the Darcy Equation in Case of Open-Cell Metal Foam Based on Numerical Simulations
Authors: De Schampheleire Sven , De Kerpel Kathleen , Ameel Bernd , De Jaeger Peter , Bagci Ozer , De Paepe Michel
Published: May 2016

It is long known that for high-velocity fluid flow in porous media, the relation between the pressure drop and the superficial velocity is not linear. Indeed, the classical Darcy law for shear stress dominated flow needs to be extended with a quadratic term, resulting in the empirical Darcy–Forchheimer model. Another approach is to simulate the foam numerically through the volume averaging technique. This leads to a natural separation of the total drag force into the contribution of the shear forces and the contribution of the pressure forces. Both representations of the total drag lead to the same result. The physical correspondence between both approaches is investigated in this work. The contribution of the viscous and pressure forces on the total drag is investigated using direct numerical simulations. Special attention is paid to the dependency on the velocity of these forces. The separation of the drag into its constituent terms on experimental grounds and for the volume average approach is unified. It is shown that the common approach to identify the linear term with the viscous forces and the quadratic term with the pressure forces is not correct.
Design of Inorganic Polymer Mortar from Ferricalsialic and Calsialic Slags for Indoor Humidity Control
Authors: Kamseu Elie , Lancellotti Isabella , Sglavo Vincenzo , Modolo Luca , Leonelli Cristina
Published: May 2016

Amorphous silica and alumina of metakaolin are used to adjust the bulk composition of black (BSS) and white (WSS) steel slag to prepare alkali-activated (AAS) mortars consolidated at room temperature. The mix-design also includes also the addition of semi-crystalline matrix of river sand to the metakaolin/steel powders. The results showed that high strength of the steel slag/metakaolin mortars can be achieved with the geopolymerization process which was particularly affected by the metallic iron present into the steel slag. The corrosion of the Fe particles was found to be responsible for porosity in the range between 0.1 and 10 µm. This class of porosity dominated (~31 vol %) the pore network of B compared to W samples (~16 vol %). However, W series remained with the higher cumulative pore volume (0.18 mL/g) compared to B series, with 0.12 mL/g. The maximum flexural strength was 6.89 and 8.51 MPa for the W and B series, respectively. The fracture surface ESEM observations of AAS showed large grains covered with the matrix assuming the good adhesion bonds between the gel-like geopolymer structure mixed with alkali activated steel slag and the residual unreacted portion. The correlation between the metallic iron/Fe oxides content, the pore network development, the strength and microstructure suggested the steel slag's significant action into the strengthening mechanism of consolidated products. These products also showed an interesting adsorption/desorption behavior that suggested their use as coating material to maintain the stability of the indoor relative humidity.
The GenePOC Platform, a Rational Solution for Extreme Point-of-Care Testing
Authors: Bissonnette Luc , Bergeron Michel
Published: May 2016

Extreme point-of-care (POC) testing for infections, as performed (endured) in low-resource settings, developing countries, tropical areas, or in conditions following emergency crises or natural disasters, must be undertaken under environmental, logistic, and societal conditions which impose a significant deal of stress on local human populations and healthcare providers. For disease diagnostics or management, simple and robust biomedical equipment and reagents are required and needed. This chapter aims to overview some of these stresses (requirements) and intends to describe some of the solutions already engineered at the heart of centripetal (centrifugal) microfluidic platforms such as that of GenePOC Inc. to enable rapid, robust, and reproducible nucleic acid-based diagnostics of infectious diseases, to better control the morbidity and mortality of infections and the expanding threat posed by antimicrobial resistance.
Compensation Method for Die Shift Caused by Flow Drag Force in Wafer-Level Molding Process
Authors: Yeon Simo , Park Jeanho , Lee Hye-Jin
Published: May 2016

Wafer-level packaging (WLP) is a next-generation semiconductor packaging technology that is important for realizing high-performance and ultra-thin semiconductor devices. However, the molding process, which is a part of the WLP process, has various problems such as a high defect rate and low predictability. Among the various defect factors, the die shift primarily determines the quality of the final product; therefore, predicting the die shift is necessary to achieve high-yield production in WLP. In this study, the die shift caused by the flow drag force of the epoxy molding compound (EMC) is evaluated from the die shift of a debonded molding wafer. Experimental and analytical methods were employed to evaluate the die shift occurring during each stage of the molding process and that resulting from the geometrical changes after the debonding process. The die shift caused by the EMC flow drag force is evaluated from the data on die movements due to thermal contraction/expansion and warpage. The relationship between the die shift and variation in the die gap is determined through regression analysis in order to predict the die shift due to the flow drag force. The results can be used for die realignment by predicting and compensating for the die shift.
ESeroS-GS Protects Neuronal Cells from Oxidative Stress by Stabilizing Lysosomes
Authors: Yang Na , Chen Qianqian , He Xiaolong , Zhao Xingyu , Wei Taotao
Published: May 2016

γ-l-glutamyl-S-[2-[[[3,4-dihydro-2,5,7,8-tetramethyl-2-(4,8,12-trimethyltridecyl)-2H-1-benzopyran-6-yl]oxy]carbonyl]-3-[[2-(1H-indol-3-yl)ethyl]amino]-3-oxopropyl]-l-cysteinylglycine sodium salt (ESeroS-GS) is a water-soluble derivative of α-tocopherol (vitamin E). We reported previously that ESeroS-GS can act as an anti-inflammatory agent and can induce cell death in breast cancer cells. However, the potential antioxidant capacities of ESeroS-GS remain elusive. Here, we measured its scavenging effects on free radicals and evaluated its protective effects on neuronal cells against oxidative stress. The results indicated that ESeroS-GS effectively scavenged both 2,2’-azinobis(3-ethylbenzothiazoline)-6-sulfonate free radicals (ABTS•+) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radicals, and attenuated H2O2-induced neuronal cell death. H2O2 treatment induced lysosomal membrane permeabilization rapidly, and caused the redistribution of lysosomal proteases, which were responsible for the neuronal cell death. ESeroS-GS abolished the interaction between tBid and the lysosomal membranes, blocked the translocation of tBid to the lysosomal membranes, decreased its oligomerization within the membrane circumstances, prevented the lysosomal membrane permeabilization, and thus attenuated the neuronal cell death. These data suggest that ESeroS-GS protected the neuronal cells from oxidative stress by stabilizing lysosomal membranes, and thus might act as a novel neuroprotector for neuronal diseases associated with oxidative stress.
Bioactive Constituents of Zanthoxylum rhetsa Bark and Its Cytotoxic Potential against B16-F10 Melanoma Cancer and Normal Human Dermal Fibroblast (HDF) Cell Lines
Authors: Santhanam Ramesh , Ahmad Syahida , Abas Faridah , Safinar Ismail Intan , Rukayadi Yaya , Tayyab Akhtar Muhammad , Shaari Khozirah
Published: May 2016

Zanthoxylum rhetsa is an aromatic tree, known vernacularly as “Indian Prickly Ash”. It has been predominantly used by Indian tribes for the treatment of many infirmities like diabetes, inflammation, rheumatism, toothache and diarrhea. In this study, we identified major volatile constituents present in different solvent fractions of Z. rhetsa bark using GC-MS analysis and isolated two tetrahydrofuran lignans (yangambin and kobusin), a berberine alkaloid (columbamine) and a triterpenoid (lupeol) from the bioactive chloroform fraction. The solvent fractions and purified compounds were tested for their cytotoxic potential against human dermal fibroblasts (HDF) and mouse melanoma (B16-F10) cells, using the MTT assay. All the solvent fractions and purified compounds were found to be non-cytotoxic to HDF cells. However, the chloroform fraction and kobusin exhibited cytotoxic effect against B16-F10 melanoma cells. The presence of bioactive lignans and alkaloids were suggested to be responsible for the cytotoxic property of Z. rhetsa bark against B16-F10 cells.
Catalysts with Cerium in a Membrane Reactor for the Removal of Formaldehyde Pollutant from Water Effluents
Authors: Gutiérrez-Arzaluz Mirella , Noreña-Franco Luis , Ángel-Cuevas Saúl , Mugica-Álvarez Violeta , Torres-Rodríguez Miguel
Published: May 2016

We report the synthesis of cerium oxide, cobalt oxide, mixed cerium, and cobalt oxides and a Ce–Co/Al2O3 membrane, which are employed as catalysts for the catalytic wet oxidation (CWO) reaction process and the removal of formaldehyde from industrial effluents. Formaldehyde is present in numerous waste streams from the chemical industry in a concentration low enough to make its recovery not economically justified but high enough to create an environmental hazard. Common biological degradation methods do not work for formaldehyde, a highly toxic but refractory, low biodegradability substance. The CWO reaction is a recent, promising alternative that also permits much lower temperature and pressure conditions than other oxidation processes, resulting in economic benefits. The CWO reaction employing Ce- and Co-containing catalysts was carried out inside a slurry batch reactor and a membrane reactor. Experimental results are reported. Next, a mixed Ce–Co oxide film was supported on an γ-alumina membrane used in a catalytic membrane reactor to compare formaldehyde removal between both types of systems. Catalytic materials with cerium and with a relatively large amount of cerium favored the transformation of formaldehyde. Cerium was present as cerianite in the catalytic materials, as indicated by X-ray diffraction patterns.
Ethanolic Extract of Acanthopanax koreanum Nakai Alleviates Alcoholic Liver Damage Combined with a High-Fat Diet in C57BL/6J Mice
Authors: Kim Haein , Park Minyoung , Shin Jae-Ho , Kwon Oran
Published: May 2016

Alcoholic and nonalcoholic liver steatosis have an indistinguishable spectrum of histological features and liver enzyme elevations. In this study, we investigated the potential of the ethanolic extract of Acanthopanax koreanum Nakai (AK) to protect against experimental alcoholic liver disease in a mouse model that couples diet and daily ethanol bolus gavage. Fifty-six C57BL/6J mice were randomly divided into seven groups: normal control (NC), alcohol control (AC), alcohol/HFD control (AH), low-dose (1%) AK in alcohol group (ACL), high-dose (3%) AK in alcohol group (ACH), low-dose AK in alcohol/HFD group (AHL), and high-dose AK in alcohol/HFD group (AHH). The AH group showed more severe damage than the AC group in terms of biochemical and molecular data that were observed in this study. The administration of AK exerted remarkable effects in: plasma ALT (p < 0.0001), total lipid (p = 0.014), TG (p = 0.0037) levels; CPT-1α (p = 0.0197), TLR4 (p < 0.0001), CD14 (p = 0.0002), IL-6 (p = 0.0264) and MCP-1 (p = 0.0045) gene expressions; and ALDH (p < 0.0001) and CAT (p = 0.0076) activities. The data suggested that at least the high dose AK might confer protection against alcoholic liver damage combined with an HFD by accelerating lipid oxidation and alcohol metabolism and by suppressing the inflammatory response, including the TLR pathway.
Synthesis and Characterization of Glutamic-Chitosan Hydrogel for Copper and Nickel Removal from Wastewater
Authors: Abdelwahab Huda , Hassan Seham , Mostafa Mohamed , El Sadek Mohamed
Published: May 2016

Chitosan was reacted with four concentrations (2.5, 5, 10 and 20 mmol) of glutamic acid resulting in four types of glutamic-chitosan hydrogels (GCs), the activity of the resulted compounds on the removal of copper(II) and nickel(II) from wastewater were tested. The results indicated that by increasing glutamic acid concentration from GCs-1 to GCs-4, the efficiency of removing Cu(II) and Ni(II) were decreased, which may be due to a decrease in the pore size of the hydrogels as a result of the increased degree of crosslinking.
The Antistaphylococcal Activity of Citropin 1.1 and Temporin A against Planktonic Cells and Biofilms Formed by Isolates from Patients with Atopic Dermatitis: An Assessment of Their Potential to Induce Microbial Resistance Compared to Conventional Antimicrobials
Authors: Dawgul Malgorzata , Baranska-Rybak Wioletta , Piechowicz Lidia , Bauer Marta , Neubauer Damian , Nowicki Roman , Kamysz Wojciech
Published: May 2016

Staphylococcus aureus (SA) colonizes the vast majority of patients with atopic dermatitis (AD). Its resistance to antibiotics and ability to form biofilms are the main origins of therapeutic complications. Endogenous antimicrobial peptides (AMPs) exhibit strong activity against SA, including antibiotic resistant strains as well as bacteria existing in biofilm form. The purpose of the present work was to determine the antistaphylococcal activity of two amphibian peptides against SA isolated from patients with AD. The AMPs demonstrated permanent activity towards strains exposed to sublethal concentrations of the compounds and significantly stronger antibiofilm activity in comparison to that of conventional antimicrobials. The results suggest the potential application of amphibian AMPs as promising antistaphylococcal agents for the management of skin infections.
Filling the Polar Data Gap in Sea Ice Concentration Fields Using Partial Differential Equations
Authors: Strong Courtenay , Golden Kenneth
Published: May 2016

The “polar data gap” is a region around the North Pole where satellite orbit inclination and instrument swath for SMMR and SSM/I-SSMIS satellites preclude retrieval of sea ice concentrations. Data providers make the irregularly shaped data gap round by centering a circular “pole hole mask” over the North Pole. The area within the pole hole mask has conventionally been assumed to be ice-covered for the purpose of sea ice extent calculations, but recent conditions around the perimeter of the mask indicate that this assumption may already be invalid. Here we propose an objective, partial differential equation based model for estimating sea ice concentrations within the area of the pole hole mask. In particular, the sea ice concentration field is assumed to satisfy Laplace’s equation with boundary conditions determined by observed sea ice concentrations on the perimeter of the gap region. This type of idealization in the concentration field has already proved to be quite useful in establishing an objective method for measuring the “width” of the marginal ice zone—a highly irregular, annular-shaped region of the ice pack that interacts with the ocean, and typically surrounds the inner core of most densely packed sea ice. Realistic spatial heterogeneity in the idealized concentration field is achieved by adding a spatially autocorrelated stochastic field with temporally varying standard deviation derived from the variability of observations around the mask. To test the model, we examined composite annual cycles of observation-model agreement for three circular regions adjacent to the pole hole mask. The composite annual cycle of observation-model correlation ranged from approximately 0.6 to 0.7, and sea ice concentration mean absolute deviations were of order 10 − 2 or smaller. The model thus provides a computationally simple approach to solving the increasingly important problem of how to fill the polar data gap. Moreover, this approach based on solving an elliptic partial differential equation with given boundary conditions has sufficient generality to also provide more sophisticated models which could potentially be more accurate than the Laplace equation version. Such generalizations and potential validation opportunities are discussed.
Tree Species Classification Using Hyperspectral Imagery: A Comparison of Two Classifiers
Authors: Ballanti Laurel , Blesius Leonhard , Hines Ellen , Kruse Bill
Published: May 2016

The identification of tree species can provide a useful and efficient tool for forest managers for planning and monitoring purposes. Hyperspectral data provide sufficient spectral information to classify individual tree species. Two non-parametric classifiers, support vector machines (SVM) and random forest (RF), have resulted in high accuracies in previous classification studies. This research takes a comparative classification approach to examine the SVM and RF classifiers in the complex and heterogeneous forests of Muir Woods National Monument and Kent Creek Canyon in Marin County, California. The influence of object- or pixel-based training samples and segmentation size on the object-oriented classification is also explored. To reduce the data dimensionality, a minimum noise fraction transform was applied to the mosaicked hyperspectral image, resulting in the selection of 27 bands for the final classification. Each classifier was also assessed individually to identify any advantage related to an increase in training sample size or an increase in object segmentation size. All classifications resulted in overall accuracies above 90%. No difference was found between classifiers when using object-based training samples. SVM outperformed RF when additional training samples were used. An increase in training samples was also found to improve the individual performance of the SVM classifier.
Multi-Decadal Monitoring of Lake Level Changes in the Qinghai-Tibet Plateau by the TOPEX/Poseidon-Family Altimeters: Climate Implication
Authors: Hwang Cheinway , Cheng Yung-Sheng , Han Jiancheng , Kao Ricky , Huang Chi-Yun , Wei Shiang-Hung , Wang Haihong
Published: May 2016

Lake levels in the Qinghai-Tibet Plateau (QTP) provide valuable records for climate change studies. We use two decades of measurements (January 1993–December 2014) from the TOPEX/Poseidon (T/P)-family satellite altimeters (T/P, Jason-1 and -2) to detect lake level variations at 23 lakes along their repeat ground tracks every 10 days. We employ an optimal processing technique to obtain quality measurements, including outlier detection, averaging and filtering. The lake level accuracies are improved by subwaveform retracking. Jason-1 delivers few measurements after waveform retracking and a cluster classification at most lakes. From January 1993 to December 2014, most lake levels in eastern Tibet rose, while those in western Tibet declined. In Qinghai, lake levels dropped before 2005 and then rose afterwards, coinciding with the measure in 2005 that protects the Qinghai ecosystem (e.g., grassland conservation). The overall pattern of lake level change in the QTP is largely affected by monsoons and lake locations. Most lake levels show clear annual and inter-annual oscillations. Certain lakes show alternating level highs and lows in the same seasons and varying amplitudes of annual oscillations due to lake level changes. We detect a sudden rise of lake level by 7 m caused by floods, varying lake level trends associated with the 1997‒98 El Niño and other factors, and persistently rising and declining lake levels associated with the long-term precipitation trends in the QTP. The T/P-family satellites will continue to monitor lake levels here as long as the sea level monitoring program lasts, collecting a long-term climate record at highlands echoing sea level change.
A Highly Reliable and Cost-Efficient Multi-Sensor System for Land Vehicle Positioning
Authors: Li Xu , Xu Qimin , Li Bin , Song Xianghui
Published: May 2016

In this paper, we propose a novel positioning solution for land vehicles which is highly reliable and cost-efficient. The proposed positioning system fuses information from the MEMS-based reduced inertial sensor system (RISS) which consists of one vertical gyroscope and two horizontal accelerometers, low-cost GPS, and supplementary sensors and sources. First, pitch and roll angle are accurately estimated based on a vehicle kinematic model. Meanwhile, the negative effect of the uncertain nonlinear drift of MEMS inertial sensors is eliminated by an H∞ filter. Further, a distributed-dual-H∞ filtering (DDHF) mechanism is adopted to address the uncertain nonlinear drift of the MEMS-RISS and make full use of the supplementary sensors and sources. The DDHF is composed of a main H∞ filter (MHF) and an auxiliary H∞ filter (AHF). Finally, a generalized regression neural network (GRNN) module with good approximation capability is specially designed for the MEMS-RISS. A hybrid methodology which combines the GRNN module and the AHF is utilized to compensate for RISS position errors during GPS outages. To verify the effectiveness of the proposed solution, road-test experiments with various scenarios were performed. The experimental results illustrate that the proposed system can achieve accurate and reliable positioning for land vehicles.
Sensitive Adsorptive Voltammetric Method for Determination of Bisphenol A by Gold Nanoparticle/Polyvinylpyrrolidone-Modified Pencil Graphite Electrode
Authors: Yaman Yesim , Abaci Serdar
Published: May 2016

A novel electrochemical sensor gold nanoparticle (AuNP)/polyvinylpyrrolidone (PVP) modified pencil graphite electrode (PGE) was developed for the ultrasensitive determination of Bisphenol A (BPA). The gold nanoparticles were electrodeposited by constant potential electrolysis and PVP was attached by passive adsorption onto the electrode surface. The electrode surfaces were characterized by electrochemical impedance spectroscopy (EIS) and scanning electron microscopy (SEM). The parameters that affected the experimental conditions were researched and optimized. The AuNP/PVP/PGE sensor provided high sensitivity and selectivity for BPA recognition by using square wave adsorptive stripping voltammetry (SWAdSV). Under optimized conditions, the detection limit was found to be 1.0 nM. This new sensor system offered the advantages of simple fabrication which aided the expeditious replication, low cost, fast response, high sensitivity and low background current for BPA. This new sensor system was successfully tested for the detection of the amount of BPA in bottled drinking water with high reliability.
Analysis of the Appropriateness of the Use of Peltier Cells as Energy Sources
Authors: Hájovský Radovan , Pieš Martin , Richtár Lukáš
Published: May 2016

The article describes the possibilities of using Peltier cells as an energy source to power the telemetry units, which are used in large-scale monitoring systems as central units, ensuring the collection of data from sensors, processing, and sending to the database server. The article describes the various experiments that were carried out, their progress and results. Based on experiments evaluated, the paper also discusses the possibilities of using various types depending on the temperature difference of the cold and hot sides.
Energy-Efficient Collaborative Outdoor Localization for Participatory Sensing
Authors: Wang Wendong , Xi Teng , Ngai Edith , Song Zheng
Published: May 2016

Location information is a key element of participatory sensing. Many mobile and sensing applications require location information to provide better recommendations, object search and trip planning. However, continuous GPS positioning consumes much energy, which may drain the battery of mobile devices quickly. Although WiFi and cell tower positioning are alternatives, they provide lower accuracy compared to GPS. This paper solves the above problem by proposing a novel localization scheme through the collaboration of multiple mobile devices to reduce energy consumption and provide accurate positioning. Under our scheme, the mobile devices are divided into three groups, namely the broadcaster group, the location information receiver group and the normal participant group. Only the broadcaster group and the normal participant group use their GPS. The location information receiver group, on the other hand, makes use of the locations broadcast by the broadcaster group to estimate their locations. We formulate the broadcaster set selection problem and propose two novel algorithms to minimize the energy consumption in collaborative localization. Simulations with real traces show that our proposed solution can save up to 68% of the energy of all of the participants and provide more accurate locations than WiFi and cellular network positioning.
Sustainable Supply Chain Capabilities: Accumulation, Strategic Types and Performance
Authors: Lee Jung , Kim Soo , Lee Su-Yol
Published: May 2016

This paper explores the cumulative sustainable supply chain (SC) capabilities and their effects on supply chain performance, including economic, environmental, and social performance. Using empirical analyses with data from 198 small- and medium-sized suppliers in Korea, this paper provides evidence about the cumulative sustainable SC capabilities, indicating that economic, social, and environmental capabilities in the supply chain mutually reinforce each other rather than traded off. This study also presents the positive effect of cumulative sustainable SC capabilities on supply chain sustainability performance. This paper identifies four distinctive groups of cumulative capabilities: the laggard, environmental-focused, social-cautious, and all-round. This study provides a better understanding about sustainable capabilities and important guidelines for managers of suppliers and buyers who wish to build strong social/environmental management capabilities without compromising economic capability throughout the entire supply chain.
Assessing Jatropha Crop Production Alternatives in Abandoned Agricultural Arid Soils Using MCA and GIS
Authors: Corral Serafin , Romero Manrique de Lara David , Tejedor Salguero Marisa , Jimenez Mendoza Carmen , Legna-de la Nuez David , Dorta Santos María , Díaz Peña Francisco
Published: May 2016

This paper discusses the assessment of various biofuel crop production alternatives on the island of Fuerteventura using Jatropha crops. It adopts an integrated approach by carrying out a multi-criteria assessment with the support of participatory techniques and geographical information systems. Sixteen production alternatives were analyzed for growing Jatropha, and the results suggest that the best alternative involves using typical torrifluvent soils irrigated with recycled urban wastewater using surface drip irrigation covering 100% evapotranspiration. It was also determined that a potential area of 2546 ha could be used for cultivation within a radius of 10 km from a wastewater treatment plant. This level of production would supply 27.56% of the biofuel needs of Fuerteventura, thereby contributing to the 2020 target of the European Commission regarding biofuels for land transport.
Occurrence of 26 Mycotoxins in the Grain of Cereals Cultivated in Poland
Authors: Bryła Marcin , Waśkiewicz Agnieszka , Podolska Grażyna , Szymczyk Krystyna , Jędrzejczak Renata , Damaziak Krzysztof , Sułek Alicja
Published: May 2016

The levels of 26 mycotoxins were determined in 147 samples of the grain of cereals cultivated in five regions of Poland during the 2014 growing season. The HPLC-HRMS (time-of-flight) analytical technique was used. An analytical procedure to simultaneously determine 26 mycotoxins in grain was developed, tested and verified. Samples from eastern and southern Poland were more contaminated with mycotoxins than the samples from northern and western Poland. Toxins produced by Fusarium fungi were the main contaminants found. Some deoxynivalenol (DON) was found in 100% of the tested samples of wheat (Osiny, Borusowa, Werbkowice), triticale, winter barley and oats, while the maximum permissible DON level (as defined in the EU Commission Regulation No. 1881/2006) was exceeded in 10 samples. Zearalenone (ZEN), DON metabolites and enniatins were also commonly found. The presence of mycotoxins in grain reflected the prevailing weather conditions during the plant flowering/earing stages, which were favorable for the development of blight. Among all investigated wheat genotypes, cv. Fidelius was the least contaminated, while Bamberka, Forkida and Kampana were the most contaminated. However, the single-factor ANOVA analysis of variance did not reveal (at a statistical significance level α = 0.05) any differences between levels of mycotoxins in individual genotypes. Triticale was the most contaminated grain among all of the tested varieties. ZEN, DON and the sum of 3-acetyldexynivalenol and 15-acetyldeoxynivalenol (3- and 15-ADON) were found in 100% of the tested triticale samples at concentrations within the 4–86, 196–1326 and 36–374 µg·kg−1 range, respectively. Of particular concern was the fact that some “emerging mycotoxins” (enniatins) (in addition to commonly-known and legally-regulated mycotoxins) were also found in the tested triticale samples (enniatin B (Enn-B), enniatin B1 (Enn-B1), enniatin A-1 (Enn-A1), 100% of samples, and enniatin A (Enn-A), 70% of samples). Depending on the toxin, they were found at levels between 8 and 3328 µg·kg−1.
Advances in Biosensors, Chemosensors and Assays for the Determination of Fusarium Mycotoxins
Authors: Lin Xialu , Guo Xiong
Published: May 2016

The contaminations of Fusarium mycotoxins in grains and related products, and the exposure in human body are considerable concerns in food safety and human health worldwide. The common Fusarium mycotoxins include fumonisins, T-2 toxin, deoxynivalenol and zearalenone. For this reason, simple, fast and sensitive analytical techniques are particularly important for the screening and determination of Fusarium mycotoxins. In this review, we outlined the related advances in biosensors, chemosensors and assays based on the classical and novel recognition elements such as antibodies, aptamers and molecularly imprinted polymers. Application to food/feed commodities, limit and time of detection were also discussed.