bluuebunny/crossref_metadata_2025 huggingface dataset:
Hydrothermal Carbonization of Corn Stover: Structural Evolution of Hydro-Char and Degradation Kinetics
Authors: Teribele Tiago , Costa Maria , Sales da Silva Conceição , Pereira Lia , Bernar Lucas , de Castro Douglas , da Costa Assunção Fernanda , Santos Marcelo , de Sousa Brandão Isaque , Fonseca Clícia , Shultze Maja , Hofmann Thomas , Bremer Sammy , Machado Nélio
Published: April 2023

Hydrothermal processing of biomass may be able to overcome a series of problems associated with the thermochemical conversion of lignocellulosic material into energy and fuels. Investigating the process parameters and an adequate process description is one of the first steps to being able to design and optimize a certain treatment concept. In the present article, we studied process evolution with respect to reaction time in order to evaluate structure changes and kinetics of corn stover decomposition in a hydrothermal reactor. The effect of the biomass-to-H2O ratio was also investigated. A pilot-scale reactor of 18.75 L was used to conduct hydrothermal processing runs at 250 °C at different reaction times (60, 120 and 240 min) and biomass-to-H2O ratios (1:10, 1:15 and 1:20). Solid phase products were characterized by thermogravimetry (TG), scanning electron microscopy (SEM), elemental composition (EDX), crystalline phases by X-ray diffraction (XRD) and surface area (BET). For the experiments with a constant reaction time, the yields of hydro-char, aqueous and gaseous phases varied between 31.08 and 35.82% (wt.), 54.59 and 60.83% (wt.) and 8.08 and 9.58% (wt.), respectively. The yields of hydro-char and gases tend to increase with higher biomass-to-H2O ratios, while aqueous phase yields are lower when using lower ratios. As expected, the yields of liquid and gases are higher when using higher reaction times, but there is a reduction in hydro-char yields. TG showed that 60 min was not enough to completely degrade the corn stover, while 120 and 240 min presented similar results, indicating an optimized time of reaction between 120 and 240 min. SEM images, elemental composition and XRD of hydro-char showed that higher biomass-to-H2O ratios increase the carbonization of corn stover. The surface area analysis of hydro-char obtained at 250 °C, 2.0 °C/min, a biomass-to-H2O ratio of 1:10 and 240 min showed a surface area of 4.35 m2/g, a pore volume of 18.6 mm3/g and an average pore width of 17.08 μm. The kinetic of corn stover degradation or bio-char formation was correlated with a pseudo-first-order exponential model, exhibiting a root-mean-square error (r2) of 1.000, demonstrating that degradation kinetics of corn stover with hot-compressed H2O, expressed as hydro-char formation, is well described by an exponential decay kinetics.
Structured Catalysts for Non-Thermal Plasma-Assisted Ammonia Synthesis
Authors: Meloni Eugenio , Cafiero Liberato , Martino Marco , Palma Vincenzo
Published: April 2023

Ammonia has been intensively studied as a clean, sustainable fuel source and an efficient energy storage medium due to its effectiveness as a hydrogen carrier molecule. However, the currently used Haber–Bosch process requires a large fossil fuel input, high temperatures and pressures, as well as a significant capital investment. These constraints prevent decentralized and small-scale ammonia production at the level of small farms and local communities. Non-thermal plasma (NTP) can promote ammonia synthesis in operating conditions in which, in a conventional process, a catalyst is generally not active. In this study, the production of NTP-assisted catalytic ammonia at milder temperatures and ambient pressure was investigated. Four different structured catalysts were prepared and tested using an experimental plant based on a dielectric barrier discharge (DBD) reactor. The effect of the gas hourly space velocity (GHSV) was investigated, as well as the effect of the N2/H2 ratio on catalyst performance. The results evidenced that the best catalytic activity (about 4 mmol h−1 of produced NH3) was obtained using the 10Ni/zeolite 13X sample with the lowest energy consumption, thus highlighting the feasibility of this innovative technology in this field.
Simulation Study on Solar Single/Double-Effect Switching LiBr-H2O Absorption Refrigeration System
Authors: Li Qingyang , Zhao Shiqi , Wang Dechang , Song Qinglu , Zhou Sai , Wang Xiaohe , Li Yanhui
Published: April 2023

In this study, a solar single/double-effect switching LiBr-H2O absorption refrigeration system was investigated to make full use of solar energy and give full play to the advantages of solar refrigeration systems. A corresponding thermodynamic dynamic mathematical model was developed. The operation characteristics of the system operating continuously for one week were analyzed. In order to highlight the advantages of the solar single/double-effect switching absorption refrigeration system, it was compared with other forms of solar refrigeration systems and compression refrigeration systems. The practical application potential of the single/double-effect switching LiBr-H2O absorption refrigeration system was evaluated from the perspective of economy and environmental effect. The results showed that the system could achieve the switching operation between single-effect mode and double-effect mode under weather conditions of high solar radiation intensity, and the daily cooling efficiency on such days was relatively high. After an auxiliary heater was added, the primary energy savings of the solar single/double-effect switching LiBr-H2O absorption refrigeration system were 25–52%, depending on the area of the collector and the volume of the storage tank. The solar fraction of the system was about 71.99% for continuous operation during the whole refrigeration season. However, the initial investment cost of the system equipment accounted for 89.66% of the total cost. Compared with the traditional compression refrigeration system, the initial investment cost of the solar single/double-effect switching LiBr-H2O absorption refrigeration system was higher, but it had a better environmental protection effect.
Day-Ahead Electricity Market Price Forecasting Considering the Components of the Electricity Market Price; Using Demand Decomposition, Fuel Cost, and the Kernel Density Estimation
Authors: Jin Arim , Lee Dahan , Park Jong-Bae , Roh Jae Hyung
Published: April 2023

This paper aims to improve the forecasting of electricity market prices by incorporating the characteristics of electricity market prices that are discretely affected by the fuel cost per unit, the unit generation cost of the large-scale generators, and the demand. In this paper, two new techniques are introduced. The first technique applies feature generation to the label and forecasts the transformed new variables, which are then post-processed by inverse transformation, considering the characteristic of the fuel types of marginal generators or prices through two variables: fuel cost per unit by the representative fuel type and argument of the maximum of Probability Density Function (PDF) calculated by Kernel Density Estimation (KDE) from the previous price. The second technique applies decomposition to the demand, followed by a feature selection process to apply the major decomposed feature. It is verified using gain or SHapley Additive exPlanations (SHAP) value in the feature selection process. In the case study, both showed improvement in all indicators. In the Korean Electricity Market, the unit generation cost for each generator is calculated monthly, resulting in a step-wise change in the electricity market price depending on the monthly fuel cost. Feature generation using the fuel cost per unit improved the forecasting by eliminating monthly volatility caused by the fuel costs and reducing the error that occurs at the beginning of the month. It improved the Mean Squared Percentage Error (MAPE) of 3.83[%]. Using the argument of the maximum PDF calculated by KDE improved the forecasting during the test period, where discrete monthly variations were not included. The resulting MAPE was 3.82[%]. Combining these two techniques resulted in the most accurate performance compared to the other techniques used, which had a MAPE of 3.49[%]. The MAPE of the forecasting with the decomposed data of the original price was 4.41[%].
Experimental Investigation on Velocity Fluctuation in a Vaned Diffuser Centrifugal Pump Measured by Laser Doppler Anemometry
Authors: Zhang Ning , Li Delin , Jiang Junxian , Gao Bo , Ni Dan , Alubokin Anthony , Zhang Wenbin
Published: April 2023

Turbulent flow, mainly originating from the rotor-stator interaction (RSI), is closely associated with the normal and safe operation of the centrifugal pump. In the current research, to clarify turbulent flow in the centrifugal pump with a vaned diffuser, the non-intrusive LDA (Laser Doppler Anemometry) system is applied to measure velocity pulsation signals at different regions when the pump operates at various flow rates. Time and frequency domain analysis methods are combined to investigate the velocity signals, and the velocity distribution around the volute tongue region is reconstructed from twenty measuring points. Results show that the velocity spectrum is characterized by the discrete components at the blade passing frequency and its higher harmonics, and it is caused by the RSI between the impeller and the diffuser. For the points in the volute spiral and diffusion sections, due to the significantly reduced RSI effect, the velocity spectrum shows an evident difference from comparison with the points between the impeller and diffuser, and the blade passing frequency is not always the dominant frequency. The comparison of velocity amplitudes and RMS* (root mean square of velocity) values at different points proves that the measuring position and flow rate affect velocity pulsations. As observed from velocity distribution reconstructed by LDA signals, high velocity regions are developed downstream of the diffuser channel for all the measured flow rates.
State-of-the-Art Review of Small Modular Reactors
Authors: Vinoya Carlo L. , Ubando Aristotle T. , Culaba Alvin B. , Chen Wei-Hsin
Published: April 2023

The decarbonization of the energy industry is one of the key pillars in the reduction of greenhouse gas emissions in the atmosphere. The energy generation industry is an important driver in the development of nations. Small modular reactors (SMRs) are considered an affordable energy generation technology that can provide clean energy that is comparable to other renewable energy technologies. However, several critical issues are established in the full adoption of and investment in SMRs in developing nations. A state-of-the-art review of the SMRs is proposed, involving key issues on technological, economic, environmental, and socio-political aspects. Methods of aggregating these factors in order to determine a country’s suitability to invest in SMRs have been explored, while also determining specific areas needing improvement.
Techno-Economic Analysis of Succinic Acid Production from Sugar-Rich Wastewater
Authors: Kim Hyunjin , Sang Byoung-In , Tsapekos Panagiotis , Angelidaki Irini , Alvarado-Morales Merlin
Published: April 2023

Succinic acid (SA) is a valuable platform chemical that can be converted into biodegradable plastics, resins, solvents, etc. The emerging biological routes for SA production are gaining more attention because they exploit the natural abilities of bacteria to fixate carbon dioxide (CO2). On the other hand, an inexpensive organic carbon source that can fulfill the energetic requirements of the microbial strain is also a significant challenge for industrial SA production. The current work presents a holistic techno-economic analysis of SA production using sugar-rich residual streams and biogas as raw materials. Simulation results showed that by establishing an integrated process, high SA production can be simultaneously achieved with biogas upgrading. The CO2 provided from biogas and carbohydrates, which are provided from organic by-products is converted into two products: biomethane (CH4 > 95%, a clean biofuel), and SA. The mass and energy balances and techno-economic indicators were simulated and calculated using SuperPro Designer®. The total capital investment and the total production cost for a facility producing 1000 tSA/year were estimated to be EUR 5,211,000 and EUR 2,339,000 per year, respectively. The total revenue was calculated to be EUR 2,811,000 per year, while the revenue due to biomethane produced, namely, 198,150 Nm3 corresponded to EUR 205,284 per year. The return on investment, payback period, and internal rate of return of the project were found to be 11.68%, 8.56 years, and 11.11%, respectively.
Future of Electric and Hydrogen Cars and Trucks: An Overview
Authors: Albatayneh Aiman , Juaidi Adel , Jaradat Mustafa , Manzano-Agugliaro Francisco
Published: April 2023

The negative consequences of toxic emissions from internal combustion engines, energy security, climate change, and energy costs have led to a growing demand for clean power sources in the automotive industry. The development of eco-friendly vehicle technologies, such as electric and hydrogen vehicles, has increased. This article investigates whether hydrogen vehicles will replace electric vehicles in the future. The results showed that fuel-cell cars are unlikely to compete with electric cars. This is due to the advancements in electric vehicles and charging infrastructure, which are becoming more cost-effective and efficient. Additionally, the technical progress in battery electric vehicles (BEVs) is expected to reduce the market share of fuel-cell electric vehicles (FCEVs) in passenger vehicles. However, significant investments have been made in hydrogen cars. Many ongoing investments seem to follow the sunk cost fallacy, where decision-makers continue to invest in an unprofitable project due to their already invested resources. Furthermore, even with megawatt charging, fuel-cell trucks cost more than battery-powered electric trucks. The use cases for fuel-cell electric trucks are also much more limited, as their running expenses are higher compared to electric cars. Hydrogen vehicles may be beneficial for heavy transport in remote areas. However, it remains to be seen if niche markets are large enough to support fuel-cell electric truck commercialization and economies of scale. In summary, we believe that hydrogen vehicles will not replace electric cars and trucks, at least before 2050.
Correction: Zhou et al. Transformer Fault Diagnosis Model Based on Improved Gray Wolf Optimizer and Probabilistic Neural Network. Energies 2021, 14, 3029
Authors: Zhou Yichen , Yang Xiaohui , Tao Lingyu , Yang Li
Published: April 2023

In the original publication [...]
Economic and Environmental Benefits of Energy Recovery from Municipal Solid Waste in Phnom Penh Municipality, Cambodia
Authors: Pheakdey Dek Vimean , Quan Nguyen Van , Xuan Tran Dang
Published: April 2023

This study assessed the energy potential, economic feasibility, and environmental performance of landfill gas (LFG) recovery, incineration, and anaerobic digestion (AD) technologies for Phnom Penh municipality in Cambodia, from 2023 to 2042. The economic analysis utilized the levelized cost of electricity (LCOE), payback period (PBP), and net present value (NPV) to evaluate the feasibility of each technology. Additionally, environmental performance was assessed following the IPCC 2006 guidelines. The results indicate that incineration produced the highest energy output, ranging from 793.13 to 1625.81 GWh/year, while the LFG and AD technologies yielded equivalent amounts of 115.44–271.81 GWh/year and 162.59–333.29 GWh/year, respectively. The economic analysis revealed an average LCOE of 0.070 USD/kWh for LFG, 0.053 USD/kWh for incineration, and 0.093 USD/kWh for AD. Incineration and LFG recovery were found to be economically feasible, with positive NPVs and a potential for profit within 8.36 years for incineration and 7.13 years for LFG. In contrast, AD technology had a negative NPV and required over 20 years to generate a return on investment. However, AD was the most promising technology regarding environmental performance, saving approximately 133,784 tCO2-eq/year. This study provides valuable technical information for policymakers, development partners, and potential investors to use in order to optimize waste-to-energy investment in Cambodia.
Pyrolytic Conversion of Cellulosic Pulps from “Lignin-First” Biomass Fractionation
Authors: Mullen Charles A. , Ellison Candice , Elkasabi Yaseen
Published: April 2023

Utilization of lignin is among the most pressing problems for biorefineries that convert lignocellulosic biomass to fuels and chemicals. Recently “lignin-first” biomass fractionation has received increasing attention. In most biorefining concepts, carbohydrate portions of the biomass are separated, and their monomeric sugar components released, while the relatively chemically stable lignin rich byproduct remains underutilized. Conversely, in lignin-first processes, a one-pot fractionation and depolymerization is performed, leading to an oil rich in phenolic compounds and a cellulosic pulp. Usually, the pulp is considered as a fermentation feedstock to produce ethanol. Herein, the results of a study where various cellulosic pulps are tested for their potential to produce valuable products via pyrolysis processes, assessed via analytical pyrolysis (py-GC), are presented. Samples of herbaceous (switchgrass) and woody biomass (oak) were subjected to both an acid-catalyzed and a supported-metal-catalyzed reductive lignin-first depolymerization, and the pulps were compared. Fast pyrolysis of the pulps produced levoglucosan in yields of up to about 35 wt %. When normalized for the amount of biomass entering the entire process, performing the lignin-first reductive depolymerization resulted in 4.0–4.6 times the yield of levoglucosan than pyrolysis of raw biomass. Pulps derived from switchgrass were better feedstocks for levoglucosan production compared with pulps from oak, and pulps produced from metal-on-carbon catalyzed depolymerization produced more levoglucosan than those from acid-catalyzed depolymerization. Catalytic pyrolysis over HZSM-5 produced aromatic hydrocarbons from the pulps. In this case, the yields were similar from both feedstocks and catalyst types, suggesting that there is no advantage to lignin fractionation prior to zeolite-catalyzed catalytic pyrolysis for hydrocarbons.
Insights into Androgen Receptor Action in Lung Cancer
Authors: Durovski Darko , Jankovic Milica , Prekovic Stefan
Published: April 2023

Sex hormones and their receptors play a crucial role in human sexual dimorphism and have been traditionally associated with hormone-dependent cancers like breast, prostate, and endometrial cancer. However, recent research has broadened our understanding by revealing connections with other types of cancers, such as lung cancer, where the androgen receptor has been found to be particularly significant. This review aims to explore the molecular mechanisms of androgen action in lung cancer pathogenesis and progression, highlighting the potential of inhibiting the androgen receptor signaling pathway as a therapeutic strategy for lung cancer treatment.
Investigation of Water Distribution and Mobility Dynamics in Recalcitrant Quercus acutissima Seeds during Desiccation Using Magnetic Resonance Methods
Authors: Chen Haiyan , Shen Yongbao
Published: April 2023

Recalcitrant seed vigor is closely related to seed moisture, so how do the water distribution and status change during seed drying? In this study, we investigated the association between water content (WC) and germination of Quercus acutissima seeds and used nuclear magnetic resonance (NMR) to monitor the water dynamics during seed drying. Results showed that freshly dispersed seeds had 38.8% WC, but drying to 14.8% WC resulted in a complete loss of vigor. Magnetic resonance images (MRI) reveal that the embryonic axis had the highest WC and the fastest rate of water loss, and seeds lost water from the embryonic axis to the apex and from the center to the end of cotyledons during desiccation. According to low-field NMR results, the proportion of free water in fresh seeds was the highest at 55%, followed by bound water at 10% and immobile water at 35%. During drying, the bound water and free water of seeds were lost simultaneously, and free water was lost most when the seeds died. Our results revealed that Q. acutissima seeds are highly sensitive to desiccation and that the water loss sites of the seeds were at the micropyle and scar. During desiccation, the bound water could not be retained, and the water balance in the seeds was broken, eventually leading to seed death.
High-Quality Assembly and Comparative Analysis of Actinidia latifolia and A. valvata Mitogenomes
Authors: Ren Wangmei , Wang Liying , Feng Guangcheng , Tao Cheng , Liu Yongsheng , Yang Jun
Published: April 2023

Kiwifruit (Actinidia) has been recently domesticated as a horticultural crop with remarkably economic and nutritional value. In this study, by combining sequence datasets from Oxford Nanopore long-reads and Illumina short-reads, we de novo assembled two mitogenomes of Actinidia latifolia and A. valvata, respectively. The results indicated that the A. latifolia mitogenome has a single, circular, 825,163 bp molecule while the A. valvata mitogenome possesses two distinct circular molecules, 781,709 and 301,558 bp, respectively. We characterized the genome structure, repeated sequences, DNA transfers, and dN/dS selections. The phylogenetic analyses showed that A. valvata and A. arguta, or A. latifolia and A. eriantha, were clustered together, respectively. This study provides valuable sequence resources for evolutionary study and molecular breeding in kiwifruit.
Crosstalk between Gut Microbiota and Hepatocellular Carcinoma
Authors: Xie Chencheng , Pocha Christine
Published: April 2023

In recent decades, gut microbiota have received emerging attention regarding their integral role in chronic liver disease progression, given the anatomic connection and the gut–liver axis. Emerging evidence has indicated a complex link between gut microbiota and hepatocellular carcinoma. This review explores the pathophysiological crosstalk between gut dysbiosis and hepatocarcinogenesis. The metabolic and immunologic effects mediated by gut-microbiota-derived metabolites, such as bile acids, short-chain fatty acids, and alcohol, could impact the aberrant biological behavior of hepatocellular carcinoma. This review also investigates the value of gut microbiota as novel non-invasive diagnostic biomarkers for the early detection of hepatocellular carcinoma, and summarizes the changes in the gut microbiota spectrum in patients with liver cancer. The current literature and studies on the role of the gut microbiota as adjuvant agents in liver cancer immunotherapy are reviewed.
Health Behaviors of Colombian First-Semester University Students in Association with Behaviors of Close Social Ties, Living Arrangement, and Time Spent with Peers
Authors: Gesualdo Chrys , Pinquart Martin , Chamorro Coneo Ana , Mebarak Chams Moises
Published: April 2023

Objective: in Colombia, many first-year university students consume unhealthy food, are physically inactive, and drink regularly, which can be associated with the behavior of social ties, living with social ties, and time with peers. The present cross-sectional study assessed the association between health behaviors of first-semester students and these factors. Method: N = 189 (Mage = 18.79; SD = 1.07; female = 68.8%) first-semester students in Colombia completed an online questionnaire investigating current and expected health behaviors as well as influencing factors. ANCOVAs, bivariate correlations, moderation analyses, and hierarchical regressions were used to analyze the data. Results: expected food consumption (stronger among participants who live with parents) as well as current and expected heavy drinking and binge drinking (stronger among participants who do not live with parents) were significantly correlated to the respective parental behavior. Current and expected drinking was significantly correlated to partners’ drinking. Expected physical activity was correlated with peers’ physical activity. Partners’ attempts to encourage drinking moderated the association between participants’ current and expected drinking with partners’ drinking. Time spent with peers was related to heavy drinking and engaging in more physical activity. Conclusion: in Colombia, parents appear to play a significant role in their offspring’s health behaviors during their first semester at university, particularly regarding food consumption and alcohol use. Partners’ drinking and time spent with peers are strongly related to heavy drinking.
The Relationship between Anger Expression and Performance Score in Parents and Coaches: The Mediating Role of Self-Efficacy and Assertiveness
Authors: Di Corrado Donatella , Sagone Elisabetta , Buscemi Andrea , Coco Marinella
Published: April 2023

Background: Youth sport provides regular physical activity for millions of children. It is a global system, which includes coaches, referees, athletes, and parent spectators; consequently, the behavior of each member can influence the experiences of others. This study aimed to investigate the mediating role of self-efficacy and assertiveness in the relationship between the degree of anger expression and the performance children’s score in a group of parents and coaches. Methods: 200 parents (96 fathers, 104 mothers) and 200 coaches (119 males, 81 females) responded to the General Self-efficacy Scale, The Scale for Interpersonal Behavior, and the State–Trait Anger Expression Inventory-2; an indicator of performance was asked of each coach. The age range of parents was 28–59 years (M = 41.39, SD = 7.07), while that of coaches was 27–43 years (M = 35.91, SD = 3.35). Results: Results revealed that self-efficacy and assertiveness were the direct and indirect predictors of performance score. Conclusions: The study provided an understanding of the nature and magnitude of this profoundly interpersonal experience. Future studies may advance relevant education programs and effective interventions aimed at reducing angry expressions and lack of communication.
Architecture of a Data Portal for Publishing and Delivering Open Data for Atmospheric Measurement
Authors: Quille Rosa Virginia Encinas , de Almeida Felipe Valencia , Ohara Mauro Yuji , Corrêa Pedro Luiz Pizzigatti , de Freitas Leandro Gomes , Alves-Souza Solange Nice , de Almeida Jorge Rady , Davis Maggie , Prakash Giri
Published: April 2023

Atmospheric data are collected by researchers every day. Campaigns such as GOAmazon 2014/2015 and the Amazon Tall Tower Observatory collect essential data on aerosols, gases, cloud properties, and meteorological parameters in the Brazilian Amazon basin. These data products provide insights and essential information for analyzing and predicting natural processes. However, in Brazil, it is estimated that more than 80% of the scientific data collected are not published due to the lack of web portals that collect and store these data. This makes it difficult, or even impossible, to access and integrate the data, which can result in the loss of significant amounts of information and significantly affect the understanding of the overall data. To address this problem, we propose a data portal architecture and open data deployment that enable Big Data processing, human interaction, and download-oriented approaches with tools that help users catalog, publish and visualize atmospheric data. Thus, we describe the architecture developed, based on the experience of the Atmospheric Radiation Measurement Data Center, which incorporates the principles of FAIR, the infrastructure and content management system for managing scientific data. The portal partial results were tested with environmental data from contaminated areas at the University of São Paulo. Overall, this data portal creates more shared knowledge about atmospheric processes by providing users with access to open environmental data.
The Mediating Role of Perceived Comfort between Workplace Attachment Style and Perceived Stress
Authors: Rebillon Justine , Codou Olivier , Hamel Jean-Félix , Moffat Eva , Scrima Fabrizio
Published: April 2023

Past studies highlight the relevance of attachment theory to the study of workplace stress and the impact of employee assessments about the physical–spatial work environment on their health. This paper is one of a number of works studying the points of connection between Bowlby’s attachment theory and the place attachment theory adopted by environmental psychologists. We proposed that a secure workplace attachment style would be negatively associated with perceived stress (and vice versa for insecure workplace attachment styles). Perceived comfort was hypothesized to mediate these effects. A convenience sample of French white-collar workers (N = 379) completed an online survey. Hypotheses were tested using the PROCESS macro. Both insecure workplace attachment styles (i.e., avoidant and preoccupied) were negatively associated with perceived comfort, which partially mediated their positive effect on perceived stress. The preventive influence of a secure workplace attachment on perceived stress was entirely mediated by its positive effect on perceived comfort. By setting different expectations regarding the work environment, workplace attachment styles could translate into a more or less stressful and comfortable employee experience. The more secure the bond employees internalize with their workplace, the more they might benefit from its comforts’ restorative potential.
Refugee Family Health Brokers’ (FHBs’) Experiences with Health Care Providers: A Thematic Analysis
Authors: Sar Bibhuti K.
Published: April 2023

Background. The resettlement and post-resettlement quality of life of refugees is often marred by chronic health/mental health conditions. To adequately care for refugees suffering these conditions, a promising strategy is the use of refugee Family Health Brokers (FHBs). FHBs are safe and trusted family members functioning as intermediaries between one’s family and health care providers. Although FHBs are known to positively influence health care utilization in their families, little is known about them and this aspect of their family caregiving role and experiences, particularly with health care providers, necessitating further research. Methods. Fourteen Bhutanese and three Bosnian refugee FHBs participated in a 2-hr focus group discussing their experiences with health care providers after being surveyed about their FHB role. Results. Thematic analysis yielded five themes centered around perceptions, knowledge, communication, behavior, and responsibilities reflective of FHBs’ experiences, which can be understood as symptoms of existing structural inequalities. Conclusions. FHBs primarily conveyed problems, struggles, and dilemmas they experienced more so than rewarding aspects of being an FHB. Suggestions are provided on how to avert these negative experiences from occurring and becoming barriers to developing allyship with FHBs in the context of existing structural inequalities.
Impact of Social Media Use on HIV Testing and Related Mediator among Men Who Have Sex with Men in Shandong Province, China
Authors: Mboussi Daisy Aurore Steinely , Lin Yuxi , Fornah Lovel , Ma Wei
Published: April 2023

In China, HIV testing is the important first step of HIV prevention and treatment cascades but is not widely adopted by men who have sex with men (MSM). However, social media has been increasingly used to promote and improve HIV testing. This study aimed to assess the impact of social media use on HIV testing and explore the mediator in the relationship between social media and HIV testing. The authors conducted a cross-sectional study among 935 MSM participants in 11 cities in Shandong Province from 14 September to 6 December 2020. Multivariable regression was conducted to assess the relationship between social media use and HIV testing uptake, and mediation analysis was used to assess the mediation effect of HIV testing self-efficacy in the relationship between social media use and HIV testing. The present study findings showed that social media use was related to HIV testing (aOR = 3.024, 95% CI: 1.869, 4.892) and HIV self-testing uptake (aOR = 1.894, 95% CI: 1.228, 2.922), but was not related to HIV facility-based testing (aOR = 1.709, 95% CI: 0.806, 3.625, p = 0.162). A mediation effect of HIV testing self-efficacy was found between social media use and HIV testing (indirect effect: 0.13, 95% CI: 0.01, 0.29), facility-based testing (indirect effect: 0.05, 95% CI: 0.003, 0.16), and self-testing uptake (indirect effect: 0.07, 95% CI: 0.01, 0.16). Social media could be an effective tool to improve HIV testing, and healthcare providers should pay more attention to HIV testing self-efficacy in HIV testing promotion.
Characteristics of High-Resource Health System Users in Rural and Remote Regions: A Scoping Review
Authors: LeBlanc Michele , McGaughey Tomoko , Peters Paul A.
Published: April 2023

A small proportion of health care users are recognized to use a significantly higher proportion of health system resources, largely due to systemic, inequitable access and disproportionate health burdens. These high-resource health system users are routinely characterized as older, with multiple comorbidities, and reduced access to adequate health care. Geographic trends also emerge, with more rural and isolated regions demonstrating higher rates of high-resource use than others. Despite known geographical discrepancies in health care access and outcomes, health policy and research initiatives remain focused on urban population centers. To alleviate mounting health system pressure from high-resource users, their characteristics must be better understood within the context in which i arises. To examine this, a scoping review was conducted to provide an overview of characteristics of high-resource users in rural and remote communities in Canada and Australia. In total, 21 papers were included in the review. Using qualitative thematic coding, primary findings characterized rural high-resource users as those of an older age; with increased comorbid conditions and condition severity; lower socioeconomic status; and elevated risk behaviors.
How Valuable Are Small Measurement Datasets in Supplementing Occupational Exposure Models? A Numerical Study Using the Advanced Reach Tool
Authors: McNally Kevin
Published: April 2023

The Advanced REACH Tool (ART) is the most detailed exposure model currently available for estimating inhalation exposures to dusts, vapours, and aerosols under a broad range of exposure scenarios. The ART follows a Bayesian approach, making use of a calibrated source–receptor model to provide central estimates of exposures and information on exposure variability from meta-analyses in the literature. Uniquely amongst exposure models, the ART provides a facility to update the baseline estimates from the mechanistic model and variance components using measurement data collected on the exposure scenario; however, in practical use, this facility is little used. In this paper, the full capability of the ART tool is demonstrated using a small number of carefully chosen case studies that each had a sufficient breadth of personal exposure measurement data to support a measurement-led exposure assessment. In total, six cases studies are documented, three where the estimate from the source–receptor model of the ART was consistent with measurement data, and a further three case studies where the source–receptor model of the ART was inconsistent with measurement data, resulting in a prior-data conflict. A simulation study was designed that involved drawing subsets of between two and ten measurements from the available measurement dataset, with estimates of the geometric mean (GM) and 90th percentile of exposures from the posterior distribution of ART compared against measurement-based estimates of these summaries. Results from this work indicate that very substantial reductions in the uncertainty associated with estimates of the GM and 90th percentile could be achieved with as few as two measurements, with results in detail sensitive to both the measurements themselves and worker and company labels associated with the measurements. For case studies involving prior-data conflicts, the estimates of the GM and 90th percentile rapidly changed as measurement data were used to update the prior. However, results suggest that the current statistical model of the ART does not allow a complete resolution of a prior-data conflict.
Overexpression of Ultrabithorax Changes the Development of Silk Gland and the Expression of Fibroin Genes in Bombyx mori
Authors: Li Jiashuang , Kong Yunhui , Sun Lingling , Tang Yaling , Sun Xia , Qin Sheng , Li Muwang
Published: April 2023

Ultrabithorax (Ubx) is a member of the Hox gene group involved in cell fate decisions, cell proliferation and organ identity. Its function has been extensively researched in Drosophila melanogaster but little is known about it in Lepidoptera. To uncover the function of Ubx in the development of lepidopterans, we constructed the Ubx overexpression (UbxOE) strain based on the Nistari strain of Bombyx mori. The UbxOE strain showed a small body size, transparent intersegmental membrane and abnormal posterior silk gland (PSG). In the current study, we focused on the effect of Ubx overexpression on the posterior silk gland. As the major protein product of PSG, the mRNA expression of fibroin heavy chain (Fib-H) and fibroin light chain (Fib-L) was upregulated three times in UbxOE, but the protein expression of Fib-H and Fib-L was not significantly different. We speculated that the overexpression of Ubx downregulated the expression of Myc and further caused abnormal synthesis of the spliceosome and ribosome. Abnormalities of the spliceosome and ribosome affected the synthesis of protein in the PSG and changed its morphology.
A Computational Biology Study on the Structure and Dynamics Determinants of Thermal Stability of the Chitosanase from Aspergillus fumigatus
Authors: Wang Qian , Liu Song , Li Kecheng , Xing Ronge , Chen Xiaolin , Li Pengcheng
Published: April 2023

Environmentally friendly and efficient biodegradation with chitosanase for degrading chitosan to oligosaccharide has been gaining more importance. Here, we studied a chitosanase from Aspergillus fumigatus with potential for production, but does not have the ideal thermal stability. The structure predicted by the Alphafold2 model, especially the binding site and two catalytic residues, has been found to have a high similarity with the experimental structure of the chitosanase V-CSN from the same family. The effects of temperature on structure and function were studied by dynamic simulation and the results showed that the binding site had high flexibility. After heating up from 300 K to 350 K, the RMSD and RMSF of the binding site increased significantly, in particular, the downward shift of loop6 closed the binding site, resulting in the spatial hindrance of binding. The time proportions of important hydrogen bonds at the binding site decreased sharply, indicating that serious disruption of hydrogen bonds should be the main interaction factor for conformational changes. The residues contributing energetically to binding were also revealed to be in the highly flexible region, which inevitably leads to the decrease in the activity stability at high temperature. These findings provide directions for the modification of thermal stability and perspectives on the research of proteins without experimental structures.
Microorganisms Involved in Hydrogen Sink in the Gastrointestinal Tract of Chickens
Authors: Cisek Agata Anna , Dolka Beata , Bąk Iwona , Cukrowska Bożena
Published: April 2023

Hydrogen sink is a beneficial process, which has never been properly examined in chickens. Therefore, the aim of this study was to assess the quantity and quality of microbiota involved in hydrogen uptake with the use of real-time PCR and metagenome sequencing. Analyses were carried out in 50 free-range chickens, 50 commercial broilers, and 54 experimental chickens isolated from external factors. The median values of acetogens, methanogens, sulfate-reducing bacteria (SRB), and [NiFe]-hydrogenase utilizers measured in the cecum were approx. 7.6, 0, 0, and 3.2 log10/gram of wet weight, respectively. For the excreta samples, these values were 5.9, 4.8, 4, and 3 log10/gram of wet weight, respectively. Our results showed that the acetogens were dominant over the other tested groups of hydrogen consumers. The quantities of methanogens, SRB, and the [NiFe]-hydrogenase utilizers were dependent on the overall rearing conditions, being the result of diet, environment, agrotechnical measures, and other factors combined. By sequencing of the 16S rRNA gene, archaea of the genus Methanomassiliicoccus (Candidatus Methanomassiliicoccus) were discovered in chickens for the first time. This study provides some indication that in chickens, acetogenesis may be the main metabolic pathway responsible for hydrogen sink.
Anti-Apoptotic Effect of Chrysophanol Isolated from Cassia tora Seed Extract on Blue-Light-Induced A2E-Loaded Human Retinal Pigment Epithelial Cells
Authors: Kim Su-Kang , Ban Ju-Yeon , Kang Hyungoo , Park Sang-il
Published: April 2023

The seeds of Cassia tora (C. tora) species mainly contain anthraquinone, anthraquinone glycoside, and naphthalene derivatives. We investigated the anti-apoptotic effects of C. tora seed extract and its isolated compounds on blue-light-induced lipofuscin (A2E)-loaded human retinal pigment epithelial (RPE) cells. For analysis of the C. tora extract, high-performance liquid chromatography method was used. A2E-loaded human retinal pigment epithelial cells and blue light were used to create excessive photo-oxidation to induce cell death. Lactate dehydrogenase (LDH) assay was used to measure cell cytotoxicity, and the mRNA expression of genes involved in apoptosis was examined to evaluate the mechanism of cell death. C. tora extract, n-hexane fraction, and chrysophanol were found to inhibit apoptotic cell death. Additionally, C. tora extract, n-hexane fraction, and chrysophanol reduced the mRNA expression of genes involved in the apoptosis pathway. C. tora and chrysophanol were considered to inhibit apoptosis and oxidative stress response. The major component of C. tora has a protective effect against apoptosis. The ingredients of C. tora can be used as therapeutic substances or to prevent diseases caused by the excessive oxidation of A2E substances in the retina, such as in age-related macular degeneration.
Ion Separation Together with Water Purification via a New Type of Nanotube: A Molecular Dynamics Study
Authors: Shi Jiao , Zhou Xin , Jia Pan , Cai Kun
Published: April 2023

We propose a CNT-based concentric twin tube (CTT) as nanochannels for both water purification and ion separation at the nanoscale. In the model, a source reservoir dealing with the solution connects three containers via the CTT that has three subchannels for mass transfer. Before entering the three subchannels, the solution in the separating zone will form three layers (the aqua cations, water, and the aqua anions, respectively) by applying a charged capacitor with the two electrodes parallel to the flow direction of the solution. Under an electric field with moderate intensity, the three subchannels in the CTT have stable configurations for mass transfer. Since the water and the two types of aqua ions are collected by three different containers, the present model can realize both ion separation and water purification. The mass transfer in the subchannels will be sped up by an external pressure exerted on the solution in the source reservoir. The physical properties of the model, e.g., water purification speed, are analyzed with respect to the effects of the electric field, the size of CTT, and the concentration of solute, such as NaCl.
Alopecia in Patients with Collagen VI-Related Myopathies: A Novel/Unrecognized Scalp Phenotype
Authors: Starace Michela , Pampaloni Francesca , Bruni Francesca , Quadrelli Federico , Cedirian Stephano , Baraldi Carlotta , Misciali Cosimo , Di Martino Alberto , Sabatelli Patrizia , Merlini Luciano , Piraccini Bianca Maria
Published: April 2023

Collagen VI-related myopathies are characterized by severe muscle involvement and skin involvement (keratosis pilaris and impaired healing with the development of abnormal scars, especially keloids). Scalp involvement and hair loss have not been reported among cutaneous changes associated with collagen VI mutations. The aim of this study is to describe the clinical, trichoscopic, and histological findings of the scalp changes in patients affected by COL VI mutations and to estimate their prevalence. Patients with Ullrich congenital muscular dystrophy were enrolled and underwent clinical and trichoscopic examinations and a scalp biopsy for histopathology. Five patients were enrolled, and all complained of hair loss and scalp itching. One patient showed yellow interfollicular scales with erythema and dilated, branched vessels, and the histological findings were suggestive of scalp psoriasis. Two patients presented with scarring alopecia patches on the vertex area, and they were histologically diagnosed with folliculitis decalvans. The last two patients presented with scaling and hair thinning, but they were both diagnosed with folliculitis and perifolliculitis. Ten more patients answered to a “scalp involvement questionnaire”, and six of them confirmed to have or have had scalp disorders and/or itching. Scalp involvement can be associated with COL VI mutations and should be investigated.
Spatial Gradients of E-Cadherin and Fibronectin in TGF-β1-Treated Epithelial Colonies Are Independent of Fibronectin Fibril Assembly
Authors: Griggs Lauren A. , Lemmon Christopher A.
Published: April 2023

Epithelial to Mesenchymal Transition (EMT) is a dynamic, morphogenetic process characterized by a phenotypic shift in epithelial cells towards a motile and often invasive mesenchymal phenotype. We have previously demonstrated that EMT is associated with an increase in assembly of the extracellular matrix protein fibronectin (FN) into insoluble, viscoelastic fibrils. We have also demonstrated that Transforming Growth Factor-β1 (TGF-β1) localizes to FN fibrils, and disruption of FN assembly or disruption of TGF-β1 localization to FN fibrils attenuates EMT. Previous studies have shown that TGF-β1 induces spatial gradients of EMT in mammary epithelial cells cultured on FN islands, with cells at free edges of the island preferentially undergoing EMT. In the current work, we sought to investigate: (a) whether FN fibril assembly is also spatially patterned in response to TGF-β1, and (b) what effects FN fibril inhibition has on spatial gradients of E-Cadherin and FN fibrillogenesis. We demonstrate that mammary epithelial cells cultured on square micropatterns have fewer E-Cadherin-containing adherens junctions and assemble more FN fibrils at the periphery of the micropattern in response to increasing TGF-β1 concentration, indicating that TGF-β1 induces a spatial gradient of both E-Cadherin and FN fibrils. Inhibition of FN fibril assembly globally diminished E-Cadherin-containing adherens junctions and FN fibrillogenesis, but did not eliminate the spatial gradient of either. This suggests that global inhibition of FN reduces the degree of both FN fibrillogenesis and E-Cadherin-containing adherens junctions, but does not eliminate the spatial gradient of either, suggesting that spatial gradients of EMT and FN fibrillogenesis are influenced by additional factors.