An international scholastic network to generate LexA enhancer-trap lines forDrosophila
Authors: Kim Ella S. , Rajan Arjun , Chang Kathleen , Gulick Clara , English Eva , Govindarajan Sanath , Rodriguez Bianca , Bloomfield Orion , Nakada Stella , Beard Charlotte , O’Connor Sarah , Mastroianni Sophia , Downey Emma , Feigenbaum Matthew , Tolentino Caitlin , Pace Abigail , Khan Marina , Moon Soyoun , DiPrima Jordan , Syed Amber , Lin Flora , Abukhadra Yasmina , Bacon Isabella , Beckerle John , Cho Sophia , Donkor Nana Esi , Garberg Lucy , Harrington Ava , Hoang Mai , Lawani Nosa , Noori Ayush , Park Euwie , Parsons Ella , Oravitan Philip , Chen Matthew , Molina Cristian , Richmond Caleb , Reddi Adith , Huang Jason , Shugrue Cooper , Coviello Rose , Unver Selma , Indelicarto Matthew , Islamovic Emir , McIlroy Rosemary , Yang Alana , Hamad Mahdi , Griffin Elizabeth , Ahmed Zara , Alla Asha , Fitzgerald Patricia , Choi Audrey , Das Tanya , Cheng Yuchen , Yu Joshua , Roderiques Tabor , Lee Ethan , Liu Longchao , Harper Jaekeb , Wang Jason , Suhr Chris , Tan Max , Luque Jacqueline , Tam A. Russell , Chen Emma , Triff Max , Zimmermann Lyric , Zhang Eric , Wood Jackie , Clark Kaitlin , Kpodonu Nat , Dey Antar , Ecker Alexander , Chuang Maximilian , Kodi Ramón , López Suzuki , Sun Harry , Wei Zijing , Stone Henry , Mudide Anish , Chi Chia Yu Joy , Silvestri Aiden , Orloff Petra , Nedumaran Neha , Zou Aletheia , Ünver Leyla , Page Oscair , Kim Minseo , Chan Terence Yan Tao , Tulloch Akili , Hernandez Andrea , Pillai Aruli , Chen Caitlyn , Chowdhury Neil , Huang Lina , Mudide Anish , Paik Garrett , Wingate Alexandra , Quinn Lily , Conybere Chris , Baumgardt Luca Laiza , Buckley Rollo , Kolberg Zara , Pattison Ruth , Shazli Ashlyn Ahmad , Ganske Pia , Sfragara Luca , Strub Annina , Collier Barney , Tamana Hari , Ravindran Dylan , Howden James , Stewart Madeleine , Shimizu Sakura , Braniff Julia , Fong Melanie , Gutman Lucy , Irvine Danny , Malholtra Sahil , Medina Jillian , Park John , Yin Alicia , Abromavage Harrison , Barrett Breanna , Chen Jacqueline , Cho Rachelle , Dilatush Mac , Gaw Gabriel , Gu Caitlin , Huang Jupiter , Kilby Houston , Markel Ethan , McClure Katie , Phillips William , Polaski Benjamin , Roselli Amelia , Saint-Cyr Soleil , Shin Ellie , Tatum Kylan , Tumpunyawat Tai , Wetherill Lucia , Ptaszynska Sara , Zeleznik Maddie , Pesendorfer Alexander , Nolan Anna , Tao Jeffrey , Sammeta Divya , Nicholson Laney , Vu Dinh Giao , Foltz Merrin , Vo An , Ross Maggie , Tokarski Andrew , Hariharan Samika , Wang Elaine , Baziuk Martha , Tay Ashley , Wong Yuk Hung Maximus , Floyd Jax , Cui Aileen , Pierre Kieran , Coppisetti Nikita , Kutam Matthew , Khurjekar Dhruv , Gadzi Anthony , Gubbay Ben , Pedretti Sophia , Belovich Sofiya , Yeung Tiffany , Fey Mercy , Shaffer Layla , Li Arthur , Beritela Giancarlo , Huyghue Kyle , Foster Greg , Durso-Finley Garrett , Thierfelder Quinn , Kiernan Holly , Lenkowsky Andrew , Thomas Tesia , Cheng Nicole , Chao Olivia , L’Etoile-Goga Peter , King Alexa , McKinley Paris , Read Nicole , Milberg David , Lin Leila , Wong Melinda , Gilman Io , Brown Samantha , Chen Lila , Kosai Jordyn , Verbinsky Mark , Belshaw-Hood Alice , Lee Honon , Zhou Cathy , Lobo Maya , Tse Asia , Tran Kyle , Lewis Kira , Sonawane Pratmesh , Ngo Jonathan , Zuzga Sophia , Chow Lillian , Huynh Vianne , Yang Wenyi , Lim Samantha , Stites Brandon , Chang Shannon , Cruz-Balleza Raenalyn , Pelta Michaela , Kujawski Stella , Yuan Christopher , Martinez Benjamin , Nuygen Reena , Norris Lucy , Nijensohn Noah , Altman Naomi , Maajid Elise , Burkhardt Olivia , Chanda Jullian , Doscher Catherine , Gopal Alex , Good Aaron , Good Jonah , Herrera Nate , Lanting Lucas , Liem Sophia , Marks Anila , McLaughlin Emma , Lee Audrey , Mohr Collin , Patton Emma , Pyarali Naima , Oczon Claire , Richards Daniel , Good Nathan , Goss Spencer , Khan Adeeb , Madonia Reagan , Mitchell Vivian , Sun Natasha , Vranka Tarik , Garcia Diogo , Arroyo Frida , Morales Eric , Camey Steven , Cano Giovanni , Bernabe Angelica , Arroyo Jennifer , Lopez Yadira , Gonzalez Emily , Zumba Bryan , Garcia Josue , Vargas Esmeralda , Trinidad Allen , Candelaria Noel , Valdez Vanessa , Campuzano Faith , Pereznegron Emily , Medrano Jenifer , Gutierrez Jonathan , Gutierrez Evelyn , Abrego Ericka Taboada , Gutierrez Dayanara , Barnes Angelica , Arms Eleanor , Mitchell Leo , Balanzá Ciara , Bradford Jake , Detroy Harrison , Ferguson Devin , Guillermo Ethel , Manapragada Anusha , Nanula Daniella , Serna Brigitte , Singh Khushi , Sramaty Emily , Wells Brian , Wiggins Matthew , Dowling Melissa , Schmadeke Geraldine , Cafferky Samantha , Good Stephanie , Reese Meg , Fleig Miranda , Gannett Alex , Cain Cory , Lee Melody , Oberto Paul , Rinehart Jennifer , Pan Elaine , Mathis Sallie Anne , Joiner Jessica , Barr Leslie , Evans Cory J. , Baena-Lopez Alberto , Beatty Andrea , Collette Jeanette , Smullen Robert , Suttie Jeanne , Rankin Anne E. , Chisholm Townley , Rotondo Cheryl , Lewis Gareth , Turner Victoria , Stark Lloyd , Fox Elizabeth , Amirapu Anjana , Park Sangbin , Lantz Nicole , Kockel Lutz 
Published: November 2022

Conditional gene regulation inDrosophilathrough binary expression systems like theLexA-LexAopsystem provides a superb tool for investigating gene and tissue function. To increase the availability of defined LexA enhancer trap insertions, we present molecular, genetic and tissue expression studies of 301 novel Stan-X LexA enhancer traps derived from mobilization of the index SX4 line. This includes insertions into distinct loci on the X, II and III chromosomes that were not previously associated with enhancer traps or targeted LexA constructs, an insertion intoptc, and eleven insertions into natural transposons. A subset of enhancer traps was expressed in CNS neurons known to produce and secrete insulin, an essential regulator of growth, development and metabolism. Fly lines described here were generated and characterized through studies by students and teachers in an international network of genetics classes at public, independent high schools, and universities serving a diversity of students, including those underrepresented in science. Thus, a unique partnership between secondary schools and university-based programs has produced and characterized novel resources inDrosophila, establishing instructional paradigms devoted to unscripted experimental science.