bluuebunny/crossref_metadata_2025 huggingface dataset:
Influencing Factors of Massive Hemorrhage and High-Grade Renal Vascular Injury after PCNL: A Retrospective Comparative Study
Authors: He Qiushi , Song Ziyan , Wang Xinrui , Hou Bingbing , Hao Zongyao
Published: November 2023

Purpose. Severe hemorrhage after percutaneous nephrolithotomy (PCNL) is a rare but alerting event. In this study, we report the factors affecting massive hemorrhage after PCNL, various levels of vascular damage during renal angiography, and the therapeutic effect of superselective renal artery embolization (SRAE). Patients and Methods. A retrospective analysis was performed on the data of 69 patients with postoperative PCNL hemorrhage who underwent SRAE from January 2010 to March 2021. Inclusion criteria for all cases were failure of conservative treatment for severe renal hemorrhage after surgery and then treatment with SRAE. In addition, 98 patients without significant hemorrhage after PCNL were randomly selected as the control group. All clinical data are confirmed by imaging and laboratory examinations. We performed univariate and multivariate analyses to find risk factors of massive hemorrhage and high-grade renal vascular injury after PCNL. Results. A total of 69 patients underwent angiography, 64 of which received SRAE due to positive hemorrhages detected by angiography. Urinary tract infection (OR (95% CI) = 11.214 (2.804∼44.842)), high blood pressure (OR (95% CI) = 5.686 (1.401∼23.083)), and no hydronephrosis (OR (95% CI) = 0.189 (0.049∼0.724)) are the most important factors leading to massive hemorrhage after PCNL. In patients who need SRAE after hemorrhage, high-grade vascular injury (grade III) is related to advanced age and decreased hemoglobin. Conclusion. During the perioperative period of PCNL, patients with a risk of hypertension, urinary tract infection, and no hydronephrosis should be strengthened to monitor their high risk of postoperative hemorrhage. For patients with postoperative hemorrhage, we can use the patient’s age and decreased hemoglobin before and after operation for analysis. In this way, individualized assessment can greatly improve the efficiency of SRAE treatment.
Sixth-Kind Chebyshev and Bernoulli Polynomial Numerical Methods for Solving Nonlinear Mixed Partial Integrodifferential Equations with Continuous Kernels
Authors: Al-Bugami Abeer M. , Abdou Mohamed A. , Mahdy Amr M. S.
Published: November 2023

In the present paper, a new efficient technique is described for solving nonlinear mixed partial integrodifferential equations with continuous kernels. Using the separation of variables, the nonlinear mixed partial integrodifferential equation is converted to a nonlinear Fredholm integral equation. Then, using different numerical methods, the Bernoulli polynomial method and the Chebyshev polynomials of the sixth kind, the nonlinear Fredholm integral equation has been reduced into a system of nonlinear algebraic equations. The Banach fixed-point theory is utilized in order to have a conversation about the nonlinear mixed integral equation’s solution, namely, its existence and uniqueness. In addition, we talk about the convergence and stability of the solution. Finally, a comparison between the two different methods and some other famous methods is presented through various examples. All the numerical results are calculated and obtained using the Maple software.
EGF-Receptor against Amphiregulin (AREG) Influences Costimulatory Molecules on Monocytes and T Cells and Modulates T-Cell Responses
Authors: Dreschers Stephan , Platen Christopher , Oppermann Louise , Doughty Caitlin , Ludwig Andreas , Babendreyer Aaron , Orlikowsky Thorsten W.
Published: November 2023

Amphiregulin (AREG) is a ligand of the epidermal growth factor receptor (EGFR) and has been shown to regulate the phagocytosis-induced cell death of monocytes in peripheral blood. AREG-dependent apoptotic signaling engages factors of the intrinsic and extrinsic apoptotic pathway, such as BCL-2, BCL-XL, and death ligand/receptor CD95/CD95L. Here, we tested the hypothesis that AREG influences costimulatory monocyte functions, which are crucial for T-cell responses. We found a stronger expression of AREG and EGFR in monocytes compared to lymphocytes. As a novel function of AREG, we observed reduced T-cell proliferation following polyclonal T-cell stimulation with OKT3. This reduction of proliferation occurred in the presence of monocytes as well as in their absence, monocyte signaling being replaced by crosslinking of OKT3. Increasing concentrations of AREG down-modulated the concentration of costimulatory B7 molecules (CD80/CD86) and HLA-DR on monocytes. In proliferation assays, CD28 expression on T cells was down-modulated on the application of OKT3 but unaltered by AREG. LcK activation, following OKT3-stimulation, was reduced in T cells that had been coincubated with AREG. The effects of AREG on T-cell phenotypes were also present when monocytes were depleted and OKT3 was crosslinked. The rearranged expression of immunological synapse proteins was accompanied by an alteration of T-cell polarization. Although the proportion of regulatory T cells was not shifted by AREG, IL-17-expressing T cells were significantly enhanced, with a bias toward TH1-polarization. Taken together, these results suggest that AREG acts as an immunoregulatory molecule at the interface between antigen-presenting cells and T cells.
Jackfruit Seed as a Natural Source for Protein and Mineral Enrichment of Yogurt
Authors: Hossain Md Akram , Dewan Md. Forshed , Billah Mir Tuhin , Ahiduzzaman Md. , Haque Md. Manjurul , Haque M. Amdadul
Published: November 2023

A significant proportion of the global population is currently suffering from protein and mineral malnutrition. Food enrichment or fortification is an effective strategy being utilized worldwide to fight malnutrition. The objective of the study was to extract protein and minerals from an underutilized natural source of jackfruit seed and to incorporate these nutrients into a widely consumed food yogurt. Protein isolation was achieved through the removal of the major component starch from jackfruit seed flour (JSF) followed by spray drying to get jackfruit seed protein isolate (JSPI). Mineral extraction was performed from the residuals after protein extraction. Four different yogurt samples were formulated enriched with varying concentrations of extracted protein (8%, 6%, 4%, and 2%) and a constant mineral concentration of 747 mg/100 g of yogurt. A plain yogurt served as the control sample (S5), which was not enriched with protein and mineral. The yogurts were successfully enriched with protein and minerals in this study. The sensory evaluation experiment suggested that the yogurt sample (S2) prepared with 6% protein and 747 mg/100 g mineral secured better sensory acceptance than any other sample prepared in this study. Shelf-life study showed that the yogurts were safe for consumption up to 12 days when stored under refrigeration temperature and 4 days when stored at room temperature.
Supplementary Data - raw Western blot images from Targeted Degradation of XIAP is Sufficient and Specific to Induce Apoptosis in MYCN-overexpressing High-risk Neuroblastoma
Authors: Choo Zhang'E , Koh Xiaoying , Wong Megan Rui En , Ashokan Ruth Minothini , Ali Ahamed Nurul Suhana Binte , Kang CongBao , Kuick Chik Hong , Chang Kenneth Tou En , Larisch Sarit , Loh Amos Hong Pheng , Chen Zhi Xiong
Published: November 2023

Supplementary Data - raw Western blot images
Figure S7 from Targeted Degradation of XIAP is Sufficient and Specific to Induce Apoptosis in MYCN-overexpressing High-risk Neuroblastoma
Authors: Choo Zhang'E , Koh Xiaoying , Wong Megan Rui En , Ashokan Ruth Minothini , Ali Ahamed Nurul Suhana Binte , Kang CongBao , Kuick Chik Hong , Chang Kenneth Tou En , Larisch Sarit , Loh Amos Hong Pheng , Chen Zhi Xiong
Published: November 2023

Supplementary Figure S7, related to Figure 5. Vincristine or topotecan works synergistically with and promotes effective dose reduction of XIAP-specific antagonist A4 in vitro.
FIGURE 1 from Targeted Degradation of XIAP is Sufficient and Specific to Induce Apoptosis in MYCN-overexpressing High-risk Neuroblastoma
Authors: Choo Zhang'E , Koh Xiaoying , Wong Megan Rui En , Ashokan Ruth Minothini , Ali Ahamed Nurul Suhana Binte , Kang CongBao , Kuick Chik Hong , Chang Kenneth Tou En , Larisch Sarit , Loh Amos Hong Pheng , Chen Zhi Xiong
Published: November 2023

XIAP is overexpressed in high-risk neuroblastoma and induces apoptosis when knocked down. A, Left, Immunoblot analysis of N-Myc and XIAP expressions in eight human neuroblastoma cell lines and noncancerous normal tissue-derived cell lines (THLE3: liver-derived cells; HS5: bone marrow–derived cells). β-actin is used as internal control. Right, Scatterplot of corresponding densitometry ratio for N-Myc and XIAP expression relative to β-actin with linear trend fit (R2 correlation = 0.76). B, Left, Representative images of XIAP IHC staining in a tissue microarray (n = 64; 20X), indicating staining intensity of XIAP with 0 = null, 1+ = low, 2+ = moderate, 3+ = high. Right, Graphical distribution of XIAP staining in MYCN-amplified neuroblastoma cases (n = 14) and non–MYCN-amplified neuroblastoma cases (n = 50; Supplementary Data S1). C, Immunoblot analysis of BE(2)-C and KELLY cell lines transduced with lentivirus encoding shRNAs targeting XIAP (shXIAP 78 and 79) or nontargeting control virus (shSCR). β-actin is used as internal control. Control virus shSCR served as negative control. D, Graphical representation showing fold change of apoptotic-positive BE(2)-C and KELLY cells transduced with shRNAs targeting XIAP (shXIAP 78 and 79) relative to cells transduced with nontargeting control (shSCR; data were expressed as mean ± SD of three independent experiments; *, P < 0.05). Apoptotic population was evaluated by flow cytometry analysis of cells subjected to AV/PI staining (mean percentages of Annexin V-positive cells are indicated). Control virus shSCR served as negative control. E, Crystal violet staining to determine cell viability and colony formation after lentiviral-induced silencing of XIAP and selected with 1 µg/mL (BE(2)-C) or 500 ng/mL puromycin (KELLY) for several weeks. Control virus shSCR served as negative control. Purple stained cells represent viable cells.
Supplementary Table S2 from Activation of KrasG12D in Subset of Alveolar Type II Cells Enhances Cellular Plasticity in Lung Adenocarcinoma
Authors: Chaudhary Priyanka , Xu Xia , Wang Guangfang , Hoj Jacob P. , Rampersad Rishi R. , Asselin-Labat Marie-Liesse , Ting Stephanie , Kim William , Tamayo Pablo , Pendergast Ann Marie , Onaitis Mark W.
Published: November 2023

List of the primers used in qPCR analysis
Supplementary Figure S5 from Activation of KrasG12D in Subset of Alveolar Type II Cells Enhances Cellular Plasticity in Lung Adenocarcinoma
Authors: Chaudhary Priyanka , Xu Xia , Wang Guangfang , Hoj Jacob P. , Rampersad Rishi R. , Asselin-Labat Marie-Liesse , Ting Stephanie , Kim William , Tamayo Pablo , Pendergast Ann Marie , Onaitis Mark W.
Published: November 2023

Sox2 overexpressing population in Sftpc-CreER; KrasG12D; LSL-Sox2 mice express proximal markers, CC10 and Krt5
Supplementary Figure S4 from Activation of KrasG12D in Subset of Alveolar Type II Cells Enhances Cellular Plasticity in Lung Adenocarcinoma
Authors: Chaudhary Priyanka , Xu Xia , Wang Guangfang , Hoj Jacob P. , Rampersad Rishi R. , Asselin-Labat Marie-Liesse , Ting Stephanie , Kim William , Tamayo Pablo , Pendergast Ann Marie , Onaitis Mark W.
Published: November 2023

Tumors from both Type II and double positive cells transplants express both Type I and Type II cells
Supplementary Figure S3 from Activation of KrasG12D in Subset of Alveolar Type II Cells Enhances Cellular Plasticity in Lung Adenocarcinoma
Authors: Chaudhary Priyanka , Xu Xia , Wang Guangfang , Hoj Jacob P. , Rampersad Rishi R. , Asselin-Labat Marie-Liesse , Ting Stephanie , Kim William , Tamayo Pablo , Pendergast Ann Marie , Onaitis Mark W.
Published: November 2023

H&E staining of tumors developed from transplant of Type II and double positive cells
Supplementary Figure S1 from Activation of KrasG12D in Subset of Alveolar Type II Cells Enhances Cellular Plasticity in Lung Adenocarcinoma
Authors: Chaudhary Priyanka , Xu Xia , Wang Guangfang , Hoj Jacob P. , Rampersad Rishi R. , Asselin-Labat Marie-Liesse , Ting Stephanie , Kim William , Tamayo Pablo , Pendergast Ann Marie , Onaitis Mark W.
Published: November 2023

Time course for double positive cells in Sftpc-creER; KRasG12D; fGFP mice
FIGURE 6 from Activation of KrasG12D in Subset of Alveolar Type II Cells Enhances Cellular Plasticity in Lung Adenocarcinoma
Authors: Chaudhary Priyanka , Xu Xia , Wang Guangfang , Hoj Jacob P. , Rampersad Rishi R. , Asselin-Labat Marie-Liesse , Ting Stephanie , Kim William , Tamayo Pablo , Pendergast Ann Marie , Onaitis Mark W.
Published: November 2023

Type I/II+ dual-positive cells share pathways with developing lung progenitors and contain potential therapeutic targets. A, Experimental outline for RNA-seq performed with sorted type I, type II, and double-positive cells. B, Heat map depicting the results of the NMF analysis of the RNA-seq dataset. The heat map shows the NMF clustering of samples according to cell types, with double-positive cells sharing NMF F0 factor with type II cells (red: enriched, blue: depleted). C, 2D Onco-GPS map showing double-positive cells lie between type I and type II cells in NMF-derived transcriptional space. D, Volcano plot showing differential gene expression between double-positive and type II cells. Type II marker and embryonic marker genes are highlighted in the plot. E, Heat map depicting ssGSEA results comparing current RNA-seq data with previously published datasets containing mouse embryonic and differentiated epithelial cells. Double-positive cells contain gene sets which are significantly enriched in both 11.5 and 17.5 lung progenitors. The scores on the right side are IC measures of association and permutation-test P values (see Materials and Methods). F, Heat map depicting ssGSEA results comparing current RNA-seq data with scRNA-seq data from mouse embryonic epithelial cells (15). Double-positive cells are significantly enriched in gene sets from AT1, AT2, Club as well as ciliated cells. G, Heat map depicting ssGSEA results using gene sets from the MSigDB shows enrichment of multiple gene sets that represent Ras/MAPK, EGFR, and Notch pathways in double-positive cells. H, RAS Onco-GPS analysis showing the projection of individual samples represented as color-coded circles. Each color contour represents different cell states associated with RAS activation. The black circles represent the nine RAS nodes representing major oncogenic pathways. Double-positive cells (PdTd) appear to be associated with the RAS/AP1 cell state/pathway.
FIGURE 5 from Activation of KrasG12D in Subset of Alveolar Type II Cells Enhances Cellular Plasticity in Lung Adenocarcinoma
Authors: Chaudhary Priyanka , Xu Xia , Wang Guangfang , Hoj Jacob P. , Rampersad Rishi R. , Asselin-Labat Marie-Liesse , Ting Stephanie , Kim William , Tamayo Pablo , Pendergast Ann Marie , Onaitis Mark W.
Published: November 2023

Notch inhibition or Sox2 upregulation suppresses tumor sphere formation in 3D culture. A, Representative fluorescent microscopy images of cultures (n = 3) showing spheres formed in Matrigel at day 14 from lineage-labeled TdTomato+ type II cells obtained by FACS sorting from Sftpc-CreER; Rosa-Tdtm and Sftpc-CreER; LSL-KrasG12D; Rosa-TdTomato mice. Sorting was carried out after 4 weeks of one dose of tamoxifen. B, Quantitative image analysis of A showing colony forming efficiency of lineage-labeled cells calculated by ImageJ software. Error bars represent the mean ± SEM values, and significance is defined as *, P ≤ 0.05; **, P ≤ 0.01; and ***, P ≤ 0.001.
FIGURE 3 from Activation of KrasG12D in Subset of Alveolar Type II Cells Enhances Cellular Plasticity in Lung Adenocarcinoma
Authors: Chaudhary Priyanka , Xu Xia , Wang Guangfang , Hoj Jacob P. , Rampersad Rishi R. , Asselin-Labat Marie-Liesse , Ting Stephanie , Kim William , Tamayo Pablo , Pendergast Ann Marie , Onaitis Mark W.
Published: November 2023

The KrasG12D-induced upregulation of type I cell markers in double-positive cells requires Notch signaling. A, Representative IF image of lung sections of indicated genotypes stained with GFP (Venus, Notch signaling reporter), Sftpc (red) and Rage (blue) staining in mouse. Note that Sftpc+Rage+ tumor cells express Venus (Notch reporter; marked with yellow arrows). B, Representative FACS density plots showing Notch+ population (left) in wild-type (Sftpc-CreER; CBF-H2B-Venus) versus mutant Kras (Sftpc-CreER; CBF-H2B-Venus; LSL-KrasG12D) mice. FACS plots on right side display type I and type II population from gated Notch+ population in wild-type and mutant Kras mice. DP, double positive. C, Quantification of type II cells in Notch+ population between wild-type and Kras-mutant mice. D, Quantification of double-positive (type I/II+) cells in Notch+ population between wild-type and Kras-mutant mice. E, Representative IF image of lung sections of indicated genotypes stained with GFP (green), Sftpc (red), and Rage (blue). Note that Sftpc+Rage+ tumor cells (marked with yellow arrows) are visible in Sftpc-CreER; KrasG12D; Rosa-fGFP mice but absent in GFP+ cells in Sftpc-CreER; LSL-KrasG12D; DNMaml1 mice. Results shown in A, B, and E are representative of three independent experiments. C and D represent data from three independent experiments. Error bars represent the mean ± SEM values and significance is defined as *, P ≤ 0.05; **, P ≤ 0.01; and ***, P ≤ 0.001.
FIGURE 1 from Activation of KrasG12D in Subset of Alveolar Type II Cells Enhances Cellular Plasticity in Lung Adenocarcinoma
Authors: Chaudhary Priyanka , Xu Xia , Wang Guangfang , Hoj Jacob P. , Rampersad Rishi R. , Asselin-Labat Marie-Liesse , Ting Stephanie , Kim William , Tamayo Pablo , Pendergast Ann Marie , Onaitis Mark W.
Published: November 2023

Activation of KrasG12D transforms type II cells to dual-positive type I and type II expressing cells. A, Experimental outline to compare type I, type II, and double-positive (type I/II+) population between wild-type and KrasG12D-mutant mice. B, Immunofluorescent images showing sections of left lobes of Sftpc-CreER; Rosa26-TdTomato (top) and Sftpc-CreER; LSL-KrasG12D; Rosa26-TdTomato (bottom) mice, 4 weeks after single dose of tamoxifen injection stained with DAPI (blue), Sftpc (green) and Rage (red). Right panel shows higher magnification of boxed area in left panels. Note that many tumor cells are Sftpc+Rage+ (marked with white arrows) in Kras-mutant mice. C, Quantitative analysis of B. D, FACS analysis for comparing type I, type II, and double-positive (type I/II+) population between wild-type and KrasG12D mice. E, Quantitative analysis of D. F, qPCR analysis for comparing the gene expression of some of the type I marker and type II marker genes between double-positive cells and total epithelial cells from Sftpc-CreER; KrasG12D; TdTomato mice. Results shown in B and D are representative of three independent experiments. C, E, and F represent data from three independent experiments. Error bars represent the mean ± SEM values and significance is defined as *, P ≤ 0.05; **, P ≤ 0.01; and ***, P ≤ 0.001.
Pegfilgrastim-Induced Aortitis in a Patient with Small-Cell Lung Cancer Who Received Immunotherapy Combined with Chemotherapy
Authors: Ito Takanori , Kanai Osamu , Saito Zentaro , Imakita Takuma , Oi Issei , Fujita Kohei , Tachibana Hiromasa , Mio Tadashi
Published: November 2023

Introduction: Granulocyte colony-stimulating factor (G-CSF), including pegfilgrastim, increases the peripheral blood leukocyte count and is widely used in clinical practice in combination with cytotoxic chemotherapy. The most frequent side effects of G-CSF are pain and fever; aortitis, in contrast, is a rare and serious side effect. Case Presentation: A 73-year-old man with small-cell lung cancer was treated with a full dose of a combination of carboplatin/etoposide/durvalumab and pegfilgrastim. The patient developed fever and right ear pain 12 days after pegfilgrastim administration and was diagnosed with aortitis by contrast-enhanced computed tomography 5 days later. Because the patient had already been administered the immune checkpoint inhibitor and had a history of hepatitis B, the patient was followed up without corticosteroid administration, and the patient’s symptoms resolved spontaneously. Conclusion: In situations where immunosuppression should be avoided, we believe that follow-up without corticosteroids for G-CSF-induced aortitis is a promising option.
Kombination aus PARP- and PD-1-Inhibition bei einem Patienten mit ösophagogastrischem Adenokarzinom
Authors: Artzenroth Jule Cecilia , Tintelnot Joseph , Haag Georg Martin , Gökkurt Eray , Steffens Johann , Stein Alexander
Published: November 2023

Einleitung: Das ösophagogastrische Adenokarzinom (EGA) ist weltweit eine der Hauptursachen für krebsbedingte Mortalität. Die therapeutischen Möglichkeiten sind für Patienten mit rezidivierenden oder metastasierten Erkrankungen begrenzt. Eine gezielte Therapie kann für ausgewählte Patienten eine geeignete Behandlung sein, ihre Wirksamkeit bleibt jedoch schwer fassbar. Falldarstellung: Hier zeigt ein 52-jähriger männlicher Patient mit fortgeschrittenem EGA vom Siewert-Typ II ein signifikantes Ansprechen auf die Kombinationstherapie mit Olaparib und Pembrolizumab. Nach der Progression im Anschluss an eine Erst- und Zweitlinientherapie, einschließlich eines PD-L1-Inhibitors (PD-L1 = Programmed Cell Death Ligand 1), wurde ein Next-Generation-Sequencing einer Tumorprobe durchgeführt, um mögliche molekulare Ziele zu identifizieren. Neben einer hohen PD-L1-Expression wurde eine Mutation in RAD51C, ein Teil des auf homologer Rekombination basierenden Reparatursystems (HDR-System), identifiziert. Infolgedessen wurde eine Therapie mit dem Poly(ADP-Ribose)-Polymerase (PARP)-Inhibitor Olaparib und dem PD-1-Inhibitor Pembrolizumab (PD-1 = Programmed Cell Death 1) eingeleitet. Es wurde ein dauerhaftes partielles Ansprechen beobachtet, das länger als 17 Monate anhielt. Ein zweites molekulares Profiling aus einer neu auftretenden subkutanen Metastase zeigte einen Verlust von FGF10, aber keine Abweichungen in der Genveränderung von RAD51C und SMARCA4. Interessanterweise zeigte die neue Läsion in 30% der Tumorzellen HER2-Positivität (Immunhistochemie (IHC) 3+ und Positivität bei der Fluoreszenz-in-situ-Hybridisierung (FISH)). Diskussion/Schlussfolgerung: In diesem Fall wurde trotz vorheriger Behandlung mit einem PD-L1-Inhibitor ein lang anhaltendes Ansprechen auf die Kombination von Olaparib und Pembrolizumab beobachtet. Dieser Fall zeigt, dass weitere klinische Studien erforderlich sind, um die Wirksamkeit von PARP-Inhibitorkombinationen bei EGA zu analysieren.
Life-Threatening Diffuse Alveolar Hemorrhage and Graft Failure in Atypical Hemolytic Uremic Syndrome with C3 Gene Mutation following Kidney Transplant
Authors: Song Ji Yeon , Oh Seung Hwan , Kim Younga
Published: November 2023

Introduction: Atypical hemolytic uremic syndrome (aHUS) is a thrombotic microangiopathy (TMA) disease entity primarily attributed to genetic or acquired abnormalities in the alternative complement pathway. TMA can manifest in kidney transplant (KT) recipients owing to various factors, resulting in diverse clinical presentations. Given its adverse effects on allograft function and patient prognosis, genetic diagnostic approaches for aHUS are essential. Although rarely associated with diffuse alveolar hemorrhage, only a few mild cases have been reported to date. In this report, we present a case of the patient who experienced recurrent and life-threatening diffuse alveolar hemorrhage shortly after KT accompanied by graft failure. Case Presentation: An 18-year-old girl who underwent deceased donor KT developed recurrent diffuse alveolar hemorrhage with acute kidney injury, leading to graft failure. Microangiopathic hemolytic anemia, thrombocytopenia, and schistocytes in blood smears suggested the presence of TMA. The patient underwent therapeutic plasma exchange, and clinical condition improved during the procedure. Genetic testing confirmed a heterozygous c.1273C>T mutation in C3 gene, leading to the diagnosis of aHUS. However, after discontinuing the plasma exchange, the patient experienced seizures, recurrent pulmonary hemorrhage, and oliguria with recurring TMA features. The patient subsequently underwent eculizumab treatment, which resulted in complete remission, although hemodialysis was continued after graft nephrectomy. Conclusion: In patients presenting with unexplained pulmonary hemorrhage and kidney injury following KT, genetic aHUS should be considered as a potential differential diagnosis for TMA.
Association between BMI and Efficacy of SGLT2 Inhibitors in Patients with Heart Failure or at Risk of Heart Failure: A Meta-Analysis Based on Randomized Controlled Trials
Authors: Zhou Yisheng , Dai Min , Huang Tongmin , Chen Bangsheng , Xiang Zhiyi , Tang Jiawen , Zheng Meixia , Guo Luyong
Published: November 2023

Introduction: This meta-analysis aimed to investigate the effect of SGLT2 inhibitors on the prognosis of patients with heart failure (HF) or at risk of HF across different body mass index (BMI). Methods: We searched PubMed, Embase, Web of Science, and Cochrane Library for all randomized controlled trials comparing SGLT2 inhibitors with placebo in patients with HF or at risk of HF and extracted relevant data up to April 2023 for meta-analysis. Results: A total of 29,500 patients were enrolled in the selected five studies. The results showed that patients treated with SGLT2 inhibitors had lower HF hospitalization (HHF) or cardiovascular (CV) mortality compared to those taking placebo (hazard ratio [HR] = 0.73, p < 0.001). Patients taking SGLT2 inhibitors also had a lower all-cause mortality rate than those taking placebo (HR = 0.85, p = 0.017). In BMI subgroup analysis, the HHF rate in the experimental group was lower than that in the control group at BMI ≤24.9 kg/m2, 25.0–29.9 kg/m2, and ≥30.0 kg/m2. There was no significant difference in CV mortality between the two groups at BMI ≤24.9 kg/m2 (HR = 0.91, p = 0.331) and 25.0–29.9 kg/m2 (HR = 0.92, p = 0.307). However, when the BMI was ≥30.0 kg/m2, CV mortality with SGLT2 inhibitors was lower than in the control group (HR = 0.79, p = 0.002). When patients had a BMI ≤24.9 kg/m2 (HR = 0.85, p = 0.033) and 25.0–29.9 kg/m2 (HR = 0.83, p = 0.046), the all-cause mortality was lower in the experimental group than in the control group. However, there was no significant difference between the 2 groups in patients with a BMI ≥30.0 kg/m2 (HR = 0.87, p = 0.094). Conclusion: SGLT2 inhibitors improve the prognosis in patients with HF or at risk of HF. This effect is affected by BMI.
Effectiveness of Mud-Pack Therapy and Mud-Bath Therapy in Osteoarthritis: A Systematic Review
Authors: Maier Gerrit Steffen , Rosar Gilbert , Dietz Günther , Hemken Norbert , Kafchitsas Konstantinos , Seeger Jörn Bengt , Horas Konstantin
Published: November 2023

Objectives: Osteoarthritis has a tremendous socioeconomic impact in terms of drug spending, hospital admissions, work productivity, and temporary or permanent incapacity. Mud therapy has been discussed as potential conservative treatment options for osteoarthritis. However, findings from several trials still remain controversial. For this reason, we aimed to systematically review the highest evidence provided by published trials to estimate the clinical effect of mud-pack and mud-bath therapy for the treatment of osteoarthritis. Methods: We searched PubMed, PEDro, and the Cochrane CENTRAL Register for Controlled Trials for articles published between 2000 and 2020 using the terms “orthopedics,” “orthopaedics,” “musculoskeletal,” “osteoarthritis,” and “mud bath,” “mud pack.” Results: Of the 19 studies included, 15 examined the effects of mud-bath therapy in knee osteoarthritis treatment. One study focused on the treatment effect of mud bath on hand osteoarthritis, another study examined treatment effects in hip and knee osteoarthritis, and two studies enrolled patients with chronic low back pain caused by lumbar spine osteoarthritis. We systematically reviewed the data obtained from the literature and summarized the results on the basis of the main outcomes. The results show significant improvements in function, quality of life, and perceived pain for patients with osteoarthritis. Conclusion: Results of randomized controlled trials suggest that mud therapy is part of a promising integrated and synergistic multidisciplinary approach in combination with other treatment forms like pharmacotherapy or physiotherapy.
“What Is the Most Important to Them?” Swiss Health Care Proxies, Nurses, and Physicians Discuss Planning Practices for Aged Care Residents Who No Longer Have Medical Decision-Making Capacity
Authors: Jones Laura , Rhyner Florent , Rutz Voumard Rachel , Figari Aguilar Fiorella , Rubli Truchard Eve , Jox Ralf J.
Published: November 2023

Introduction: Multiple morbidities, including neurodegenerative diseases such as dementia, which result in diminished decision-making capacity (DMC), make care and care planning complicated for residential aged care facility (RACF) residents. While advance care planning has been highlighted as essential for ensuring that this population receives care that is coherent with their wishes, there are few models specifically designed for this population. This study aimed to explore the current practices for care planning and decision-making for Swiss RACF residents who no longer have medical DMC. Methods: Semi-structured focus groups were conducted with 23 nurses in three RACFs and with 13 physicians working in 9 RACFs. Semi-structured interviews were conducted with 18 health care proxies of 16 residents without DMC. Thematic analysis was conducted to identify the most salient themes across the dataset. Results: Analyses identified many collaborative processes between nurses, physicians, and health care proxies including family meetings, reconstructing residents’ presumed will, making anticipatory decisions, and documenting these decisions. These processes were, however, highly variable and differed between institutions and between residents within each facility, with a lack of standardization. This variability was highlighted to be problematic for information transmission within facilities and in case of hospital or facility transfer. Conclusions: These results highlight the importance of standardized yet flexible processes of care planning for people who no longer have DMC and provide insights for the development of such models specifically designed to address this problem.
Business Innovation of the Local Snacks Industry in Jember City, East Java
Authors: Kantun Sri , Sedyati Retna Ngesti , Tiara Tiara , Mujib Muhammad Asyroful , Triyanto Jefri Rieski
Published: November 2023

This descriptive qualitative research describes the findings in the field of innovation carried out by the souvenir home industry in Jember Regency, which includes product innovation, service/marketing innovation, and process innovation. This research was conducted in May 2022, with the target subjects being the owners of the local snacks industry. This study uses interviews, observation, and documentation techniques to obtain data on development of the number of local snacks industries, turnover, and regulations related to food/processing companies. The results indicate that the COVID-19 pandemic has threatened business continuity in the three local snacks industries due to decreased revenue and product sales and delays in product marketing and distribution. This indicates that product innovation can lead to easier production; product innovation is also important for effective marketing and, consequently, the company's financial performance. Therefore, business innovation needs to be done in the context of business development, especially local snacks products, so that they can compete with other local snacks household industries.
Assessment of indicators of bone tissue resorption in patients with chronic generalized periodontitis according to the data of cone-beam computer tomography
Authors: Hasiuk Р. A. , Korobeinikova Yu. L. , Piasetska L. V. , Korobeinikov L. S. , Demkovych A. Ye. , Kalashnikov D. V. , Zubchenko S. G.
Published: November 2023

Summary. One of the criteria for assessing the degree of pathological changes in periodontal tissues is resorption of the bone tissue of the alveolar process of the jaw. However, changes from the vestibular and oral surfaces on a panorama or focused intraoral x-rays are covered by the shadow of the teeth and objectively do not provide an opportunity for accurate diagnosis of pathological changes in periodontal tissues. The only technique that makes it possible to obtain a three-dimensional image of the studied area with minimal exposure is cone-beam computed tomography (CBCT).

The aim of this study – to evaluate bone tissue resorption in the area of frontal teeth of patients with chronic generalized periodontitis according to CBCT data.

Materials and Methods. The study analyzed the resorption of bone tissue in the area of frontal teeth (80 teeth) in patients with chronic generalized periodontitis. The average age of the subjects was 34–44 years. The EzD2009 software and the basic interface to work in the main MPR (multiplenar reformation) option – multiplenar visualization of a three-plane image was used for CBCT analysis, with adjustment of the thickness of the selected layer (TH) from 0.03 mm to 3.0 cm.

Results and Discussion. The results of cone-beam computed tomography revealed a significant difference in the bone resorption rates of the alveolar process of the jaw from the medial, distal, and vestibulo-oral sides.

Conclusions. This indicates that a detailed study of this indicator is an essential factor in the diagnosis of periodontal changes in patients with chronic generalized periodontitis.
Порівняльна оцінка діагностичних методів визначення розмірів (площі) стравохідного отвору діафрагми у хворих із параезофагеальною грижею
Authors: ІОФФЕ О. Ю. , ТАРАСОВ Т. А.
Published: November 2023

Мета роботи: порівняти площу стравохідного отвору діафрагми у хворих із параезофагеальними грижами ІІІ типу за даними доопераційного та інтраопераційного вимірювань.

Матеріали і методи. У хірургічній клініці Національного медичного університету впродовж 2016–2021 р. проведено вимірювання площі стравохідного отвору діафрагми  у 63 хворих з параезофагеальними грижами  ІІІ типу за допомогою мультиспіральної комп’ютерної томографії, езофагогастрофіброскопії та інтраопераційно під час виконання планової лапароскопічної герніо­пластики (за методом Granderath та визначення площі ромбу). Серед хворих було 41,3 % чоловіків та 58,7 % жінок, середній вік становив (53,8±10,8) року і ІМТ (29,6±4,9) кг/м2.

Результати досліджень та їх обговорення. Встановлено сильний зв’язок між значеннями мультиспіральної комп’ютерної томографії та  іншими методами: з езофагогастрофіброскопії – лінійний R2=0,843, з методикою Granderath – R2=0,872, з методикою визначення площі ромбу –R2=0,778. Водночас величини площі стравохідного отвору діафрагми виявили певну відмінність залежно від застосованого методу.

Найбільше середнє значення площі стравохідного отвору діафрагми  порівняно з іншими методиками (всі p<0,01) отримано за даними мультиспіральної комп’ютерної томографії  – (13,01±3,04) см3 (від 8,4 см3 до 29,1 см2).  Причому площа стравохідного отвору діафрагми,  за даними мультиспіральної комп’ютерної томографії,  виявилася більшою порівняно з іншими методиками у кожного хворого. Зокрема, її результати перевищували такі отримані за методикою Granderath в середньому на (33,2±5,1) % (від 15 % до 54 %).

Найменше середнє значення площі стравохідного отвору діафрагми  отримано при езофагогастрофіброскопії  – (7,88±1,63) см2 (від 5,2 до 15,2 см2), яке достовірно відрізнялося від такої, виміряної за  методикою визначення площі ромбу – (8,72±1,67) см2 (від 5,8 до 15,4 см2), та статистично не відрізнялося за показниками, отриманими за методикою  Granderath – (8,16±1,71) см2 (від 5,8–16,3 см2). Середні значення площі стравохідного отвору діафрагми за інтраопераційними методиками статистично не відрізнялися (р>0,05).

Середні значення площі стравохідного отвору діафрагми  у хворих із параезофагеальними грижами ІІІ типу відрізняються залежно від застосованої методики вимірювання: найбільше отримано за даними мультиспіральної комп’ютерної томографії  – (13,01±3,04) см3, найменше – при езофагогастрофіброскопії – (7,88±1,63) см2. Середні значення площі стравохідного отвору діафрагми при інтраопераційному вимірюванні: за Granderath  (8,16±1,71) см2 та за  методикою визначення площі ромбу (8,72±1,67) см2  статистично не відрізнялися.
ЗМІНИ ПРОЦЕСІВ ПЕРОКСИДНОГО ОКИСНЕННЯ ЛІПІДІВ У КРОВІ ЩУРІВ-САМЦІВ, ЯКІ ЗАЗНАЛИ СТРЕСУ
Authors: Денефіль О. В. , Мозгова Є. В. , Ярошенко Т. Я. , Пришляк А. М. , Юріїв К. Є.
Published: November 2023

Вступ. Стрес на сьогодні є однією з основних проблем сьогодення не тільки в Україні, але й у всьому світі. Щоправда, стрес призводить до ушкодження різних органів і систем не в усіх, у частини відбувається адаптація до нього. Вивчення впливу різних моделей стресу, зокрема з’ясування механізмів їх дії на організм, є актуальним.
Мета дослідження – оцінити активність процесів пероксидного окиснення ліпідів у крові щурів-самців, які зазнали стресу.
Методи дослідження. Досліди виконано на 48 білих щурах-самцях лінії Вістар віком 3,5–4 місяці. Їх поділили на чотири групи: 1-ша – контроль; 2-га – стрес 24 год 3 рази (Стрес 1); 3-тя – стрес 24 год 4 рази (Стрес 2); 4-та – стрес 72 год 4 рази (Стрес 3). Стрес викликали шляхом м’якої фіксації тканиною тварини на дощечці протягом 1 год спинкою донизу. В 2-й групі спричиняли стрес кожних 24 год 3 рази, у 3-й – кожних 24 год 4 рази, в 4-й – кожних 72 год 4 рази. У сироватці крові визначали супероксиддисмутазну (СОД) і каталазну (КАТ) активність, концентрацію дієнових, трієнових кон’югатів (ДК, ТК), основ Шиффа (ОШ), ТБК-активних продуктів (ТБК-ап).
Результати й обговорення. При Стресі 1, порівняно з контролем, зросли показники ДК, ТК, ОШ, ТБК-ап. Відмічено підвищення СОД і КАТ активності. При Стресі 2, порівняно з контролем, вміст ДК збільшився і не відрізнявся від значення тварин 2-ї групи. У цій групі щурів рівень ТК виявився вищим порівняно з контролем і був нижчим, ніж у 2-й групі. Вміст ОШ зріс та був більшим порівняно з 2-ю групою. Рівень ТБК-ап, порівняно з контролем, підвищився і був більшим, ніж у 2-й групі. Порівняно з контролем СОД активність не змінилася та була нижчою, ніж у 2-й групі, КАТ активність, порівняно з контролем, знизилася і була меншою, ніж у 2-й групі. При Стресі 3 вміст ДК, порівняно з контролем, зріс та був більшим, ніж у 2-й і 3-й групах. При Стресі 3 рівень ТК перевищував показники контролю, 2-ї і 3-ї груп. Вміст ОШ знизився та був меншим порівняно з 2-ю і 3-ю групами. Рівень ТБК-ап, порівняно з контролем, підвищився та був більшим, ніж у 2-й і 3-й групах. Порівняно з контролем СОД активність різко знизилася і була меншою, ніж у 2-й і 3-й групах, КАТ активність, порівняно з контролем, зросла та була більшою, ніж у 2-й і 3-й групах.
Висновки. При стресі, що повторюється тричі кожних 24 год, виникає адаптація тварин, при стресі, який повторюється 4 рази кожних 24 год, – їх дизадаптація, при стресі, що повторюється 4 рази кожних 72 год, – виснаження.
The Concept of Complexion in Antonio da Parma’s Medical Anthropology
Authors: Robert Aurélien
Published: November 2023

Antonio da Parma (d. 1327) was a philosopher and physician, active in Bologna in the early fourteenth century, and associated with so-called “Bolognese Averroism.” His philosophical works are increasingly better documented. While his medical works are much less studied, his commentary – written between 1310 and 1323 – on the first book of Avicenna’s Canon, had a considerable influence on later commentators. This paper presents his analysis of the notion of ‘complexion’, a notion central to his anthropology for the philosophical issues it seeks to address: the possibility, for example, of defining the specific nature of the human body – as compared with other natural species – or of conceiving a scientific and universal discourse when confronted with the extreme variability of individual bodily complexion, which is at the heart of medical practice. Taking from Galen and Avicenna their ‘relativistic’ analysis of the well-balanced complexion, Antonio uses the idea of a latitude of individual complexion within the limits set by the natural species, to thereby make this picture of the human body coherent with the principles of Aristotelian natural philosophy. In so doing, he addresses the relationship between matter and form in a human body, the individuation of human bodies, or the principle of identity of a singular body. The paper concludes with a transcription of the relevant passages from Antonio’s commentary on Avicenna’s Canon.
Tedious Work: Developing Novel Outcomes with Digitization in the Arts and Sciences
Authors: Bruns Hille C. , Long Lingo Elizabeth
Published: November 2023

Tedious work is pervasive in creative work, yet it has received little attention in the literature on creativity, including studies of science, innovation, and product development. Drawing from a comparative ethnography of two settings—systems biology and music production—we illuminate tedious work as an essential, previously under-investigated aspect of creative work that becomes increasingly prominent with digitization. Tedious work is repetitive, detail-oriented, and expertise-based, and we classify four types of it: fishing, administrating, polishing, and compiling. We develop a model of how tedious work emerges, why it becomes problematic, and what actors do to reduce its negative effects. Tedious work presents three risks to developing viable, novel outcomes—time drain, disengagement, and information overload—and we identify tactics that actors use to mitigate these risks and support individual creativity and the collective creative process. By unpacking the central notion of iteration and documenting the repercussions of creating novel outcomes with digitization, specifically the potential to amplify tedious work, we provide an important counterpoint to voices that hail digital technology’s low cost and unlimited potential for iteration and refinement.
Gender consciousness and sun protection patterns among Chinese youth: A cross-sectional analysis
Authors: Li Jiaxing , Zhang Rui , Luo Yingyi , Na Lixin
Published: November 2023

Objective:

In contrast to previous research that has primarily focused on gender differences in health behaviour compliance, this study explored the association between gender consciousness and sun protection in China – a cultural setting in which there is a pronounced gender bias towards sun protection. These article is the first attempt to examine gender norms concerning sunscreen use among Chinese individuals, encompassing both young women and young men.

Design and setting:

Cross-sectional analysis conducted on one Chinese campus.

Methods:

Utilising data from the 2020 cohort of first-year students (549 male students and 1,489 female students) at a local university in Shanghai and employing OLS regression analysis, our analysis sheds light on gendered patterns of sun protection use among young individuals in China.

Results:

Findings reveal that gender attitudes affect women’s sun protection frequency without influencing their choice of specific products. Conversely, men’s gender conciousness shapes their sun protection methods but not their overall frequency of sun protection. In addition, despite lower aggregate levels of sun protection, young women exhibit higher heterogeneity in their responses than young men. Socioeconomic status, health awareness and gender consciousness are all significant factors influencing sun protection among female students; however, among men, only health awareness shows a positive correlation with the use of sun protection.

Conclusion:

The results of this study exhibit some differences from initial expectations, suggesting that the full adoption of sun protection measures is still a work in progress. However, despite the differences identified, findings provide valuable insights to inform future public health efforts to promote sun protection among youth.
Supporting the Bereaved in the COVID-19 Era: A Scoping Review of Interventions
Authors: Mueller Heidi , Corless Inge B. , Bell Jessica G. , Smeding Ruthmarijke , Anewalt Patricia , Kerslake Debbie , Lee Geok Ling , Cox Gerry , Papadatou Danai , Penny Alison , Becker Carl B. , Connor Stephen R.
Published: November 2023

People whose family member(s) friend(s) have died from COVID-19 or other causes have been deeply affected by the physical and social restrictions imposed during the pandemic. These limitations have affected end-of-life care and support for the bereaved. The purpose of this review is to identify: the published studies of evaluated programs about interventions for people who have experienced bereavement during the COVID-19 pandemic, and to develop recommendations for researchers and policy makers. Using scoping review methodology, a literature review was undertaken for articles published from January 1, 2020 through February 28, 2023 to identify interventions shown to be beneficial to people who have experienced the death of loved ones during the COVID-19 pandemic. The search yielded 1588 articles of which three studies met the criteria of utilizing a pre and post-test design with only one of these, a randomized controlled trial. The interventions included in this review demonstrate preliminary efficacy.
Use of Topical Phenol in Awake Young Children for Tympanostomy Tube Placement
Authors: Rimell Franklin L. , Cofer Shelagh , Truitt Theodore , Nimmons Grace , Raisen Jay
Published: November 2023

Importance: Phenol kits cleared by the Food and Drug Administration (FDA) are indicated as a topical anesthetic for the tympanic membrane (TM) in adults. However, there is no existing literature that reports outcomes to support the safety and use of phenol on the TM of awake children. Objective: Determine if topical phenol is safe and at low risk for complications and therefore be used effectively in awake children to facilitate office otologic procedures as in adults. Design, Setting, and Participants: Children under 21 years of age whose parents agreed to participate in an awake office setting for tympanostomy tube (TT) placement. All children had TT placement after phenol placement on the TM prior to insertion. Main Outcomes and Measures: TM perforation or other signs of TM complications through a minimum of 6-month clinical follow-up, along with assessment of the tolerability of the procedure by the child. Results: A total of 228 children with an age range of 6 months to 15.9 years and 435 TMs completed TT placement using phenol as a local anesthetic while awake in the office. There were no complications reported in the 204 children at the first follow-up visit post TM placement within 3 to 10 weeks. Of the 93 children followed up at least 6 months, there were no TM complications reported. Conclusions: This is the first study to report the outcomes on the use of phenol in an office setting in children. In this large experience, phenol appears to be tolerable and safe for use in young children in the office and is a potential safe choice of topical anesthesia for surgeons if they choose to perform office procedures such as myringotomies or TT placement on children.