bluuebunny/crossref_metadata_2025 huggingface dataset:
Signal-to-Noise Ratio Based Fault Detection and Identification
Authors: Rojas Alejandro J. , Garcés Hugo O.
Published: March 2022

In this work, we introduce signal-to-noise ratio (SNR) based fault detection and identification mechanisms for a networked control system feedback loop, where the network component is represented by an additive white noise (AWN) channel. The SNR approach is known to be a steady-state analysis and design tool, thus we first introduce a finite time approximation for the estimated AWN channel SNR. We then consider the case of a general linear time-invariant plant model with one unstable pole. The potential faults that we discuss here cover simultaneously the plant model gain and/or the unstable pole. The fault detection is performed relative to the estimated AWN channel SNR. The fault identification is performed using recursive least squares ideas and then further validated with the observed SNR value, when a fault has been previously detected. We show that the proposed SNR-based fault mechanism (fault detection plus fault identification) is capable of processing the proposed faults. We conclude discussing future research based on the contributions exposed in the present work.
Trends and Outcomes of Aortic Valve Replacement in Patients With Diabetes in the US
Authors: Khan Sarah , Dargham Soha , Al Suwaidi Jassim , Jneid Hani , Abi Khalil Charbel
Published: March 2022

Aims

We aimed to assess the trend and outcome of aortic valve replacement in patients with diabetes.

Background

Diabetes is associated with higher cardiovascular events.

Methods

Data from the National Inpatient Sample was analyzed between 2012 and 2017. We compared hospitalizations and in-hospital cardiovascular outcomes in patients with diabetes to those without diabetes, hospitalized for aortic valve replacement.

Results

In diabetes patients undergoing TAVR, the mean age of participants decreased from 79.6 ± 8 to 67.8 ± 8, hospitalizations increased from 0.97 to 7.68/100,000 US adults (p < 0.002 for both). There was a significant temporal decrease in mortality, acute renal failure (ARF), and stroke. Compared to non-diabetic patients, those with diabetes had a higher risk of stroke, ARF, and pacemaker requirement [adjusted OR = 1.174 (1.03–1.34), 1.294 (1.24–1.35), 1.153 (1.11–1.20), respectively], but a similar adjusted mortality risk. In diabetes patients undergoing sAVR, the mean age of participants decreased from 70.4 ± 10 to 68 ± 9 (p < 0.001), hospitalizations dropped from 7.72 to 6.63/100,000 US adults (p = 0.025), so did mortality, bleeding, and ARF. When compared to non-diabetes patients, those with diabetes were older and had a higher adjusted risk of mortality, stroke, and ARF [adjusted OR= 1.115 (1.06–1.17), 1.140 (1.05–1.23), 1.217 (1.18–1.26); respectively].

Conclusion

The recent temporal trend of aortic valve replacement in patients with diabetes shows a significant increase in TAVR coupled with a decrease in sAVR. Mortality and other cardiovascular outcomes decreased in both techniques. sAVR, but not TAVR, was associated with higher in-hospital mortality risk.
The Mechanism Underlying the Influence of Indole-3-Propionic Acid: A Relevance to Metabolic Disorders
Authors: Zhang Binbin , Jiang Minjie , Zhao Jianan , Song Yu , Du Weidong , Shi Junping
Published: March 2022

The increasing prevalence of metabolic syndrome has become a serious public health problem. Certain bacteria-derived metabolites play a key role in maintaining human health by regulating the host metabolism. Recent evidence shows that indole-3-propionic acid content can be used to predict the occurrence and development of metabolic diseases. Supplementing indole-3-propionic acid can effectively improve metabolic disorders and is considered a promising metabolite. Therefore, this article systematically reviews the latest research on indole-3-propionic acid and elaborates its source of metabolism and its association with metabolic diseases. Indole-3-propionic acid can improve blood glucose and increase insulin sensitivity, inhibit liver lipid synthesis and inflammatory factors, correct intestinal microbial disorders, maintain the intestinal barrier, and suppress the intestinal immune response. The study of the mechanism of the metabolic benefits of indole-3-propionic acid is expected to be a potential compound for treating metabolic syndrome.
Association Between METS-IR and Prehypertension or Hypertension Among Normoglycemia Subjects in Japan: A Retrospective Study
Authors: Han Kai-Yue , Gu Jianing , Wang Zhangsheng , Liu Jie , Zou Su , Yang Chen-Xi , Liu Dan , Xu Yingjia
Published: March 2022

Aim

Our study aimed to investigate the association between the novel non–insulin‐based metabolic score for insulin resistance (METS-IR) index and pre-hypertension (HTN) or HTN in normoglycemia Japanese participants.

Methods

The NAGALA medical examination program at Murakami Memorial Hospital in Gifu, Japan was found in 1994. 15,453 participants enrolled in this program from 2004 to 2015 was included in this retrospective study to explore the association between the METS-IR index and pre-HTN or HTN. Covariates included serum biomarkers and clinicodemographic characteristics. Logistic regression was applied to explore the association between METS-IR level and pre-HTN or HTN.

Results

This study includes a total of 15453 participants. The prevalence rates of pre-HTN and HTN were 28.55% (4412/15453) and 6.23% (962/15453), respectively. Adjusted for confounding factors in the multivariable logistic regression analysis models, when METS-IR was used as a categorical variable, high METS-IR was significantly associated with both pre-HTN (adjusted odds ratio (OR) = 1.95, 95% confidence interval (CI): 1.61–2.36) and HTN (adjusted OR = 2.12, 95% CI: 1.44–3.11). When METS-IR was used as a continuous variable, each 1 unit increase in METS-IR was associated with a 7% increase in the prevalence of pre-HTN (adjusted OR = 1.07, 95% CI: 1.06–1.08) and with a 13% increase in the prevalence of HTN (adjusted OR = 1.13, 95% CI: 1.10–1.16). Stratified analyses indicated a positive correlation between METS-IR and pre-HTN or HTN in normoglycemia subjects with different characteristics.

Conclusions

METS-IR levels are significantly associated with pre-HTN or HTN in normoglycemia individuals in Gifu, Japan. METS-IR may be used as a monitoring indicator for the development of HTN primary prevention and management strategies in the future, but it still needs more research to confirm.
Role of Dapagliflozin and Liraglutide on Diabetes-Induced Cardiomyopathy in Rats: Implication of Oxidative Stress, Inflammation, and Apoptosis
Authors: El-Shafey Mohamed , El-Agawy Mosaab Salah El-din , Eldosoky Mohamed , Ebrahim Hasnaa Ali , Elsherbini Dalia Mahmoud Abdelmonem , El-Sherbiny Mohamed , Asseri Saad Mohamed , Elsherbiny Nehal M.
Published: March 2022

The current study aims to assess the protective effects of dapagliflozin (Dapa; a sodium-glucose cotransporter-2 inhibitor) and/or liraglutide (Lira; a glucagon-like peptide 1 agonist) in an experimental model of diabetic cardiomyopathy (DCM). A single dose of streptozotocin (STZ) was administrated to male Sprague–Dawley rats by intraperitoneal injection at a dose of 50 mg/kg to induce diabetes mellitus (DM). Dapa (1 mg/kg, orally), Lira (0.4 mg/kg, s.c.), and Dapa–Lira combination were administrated for 8 weeks once-daily. Blood samples were evaluated for glucose level and biochemical markers of cardiac functions. Cardiac tissue was dissected and assessed for redox homeostasis (malondialdehyde (MDA), glutathione (GSH), and catalase (CAT)), pro-inflammatory mediators (NF-κB and tumor necrosis factor-α (TNF-α)), and apoptotic effectors (caspase-3). Moreover, the effect of treatments on the cardiac cellular structure was studied. Dapa and/or Lira administration resulted in significant improvement of biochemical indices of cardiac function. Additionally, all treatment groups demonstrated restoration of oxidant/antioxidant balance. Moreover, inflammation and apoptosis key elements were markedly downregulated in cardiac tissue. Also, histological studies demonstrated attenuation of diabetes-induced cardiac tissue injury. Interestingly, Dapa–Lira combination treatment produced a more favorable protective effect as compared to a single treatment. These data demonstrated that Dapa, Lira, and their combination therapy could be useful in protection against DM-accompanied cardiac tissue injury, shedding the light on their possible utilization as adjuvant therapy for the management of DM patients.
Platelet Derived Vesicles Enhance the TGF-beta Signaling Pathway of M1 Macrophage
Authors: Song Nan , Pan Kaifeng , Chen Lei , Jin Keke
Published: March 2022

Macrophages, mainly divided into M1 pro-inflammatory and M2 anti-inflammatory types, play a key role in the transition from inflammation to repair after trauma. In chronic inflammation, such as diabetes and complex bone injury, or the process of certain inflammatory specific emergencies, the ratio of M1/M2 cell populations is imbalanced so that M1-macrophages cannot be converted into M2 macrophages in time, resulting in delayed trauma repair. Early and timely transformation of macrophages from the pro-inflammatory M1-type into the pro-reparative M2-type is an effective strategy to guide trauma repair and establish the original homeostasis. We prepared purified nano-platelet vesicles (NPVs) and assessed their effects on macrophage phenotype switching through transcriptome analysis. The results elucidate that NPVs promote pathways related to angiogenesis, collagen synthesis, cell adhesion, and migration in macrophages, and we speculate that these advantages may promote healing in traumatic diseases.
Mechanical Booster Pump-Assisted Thermochemical Mode for Low-Grade Heat Storage and Upgrading: A Thermodynamic Study
Authors: Zeng Tao , Li Jun , Deng Lisheng , He Zhaohong , Kobayashi Noriyuki , Wu Rongjun , Huang Hongyu
Published: March 2022

To assure stable and dependable functioning of the thermochemical energy storage (TCES) system under unstable low-grade heat temperatures, three mechanical booster pump-assisted TCES (MBP-assisted TCES) modes operating with SrBr2·H2O/H2O, LiOH/H2O, and CaCl2·H2O/H2O are proposed for the application of heat storage and upgrading. The operating modes are the MBP-assisted charging mode (A mode), MBP-assisted discharging mode (B mode), and MBP-assisted charging and discharging mode (C mode). A thermodynamic model is established to evaluate the influences of condensing temperature, compression ratio, MBP isentropic efficiency, and reaction advancement on the heat source temperature and system performance from both energy and exergy perspectives. The results indicate that compared with the other two modes, the B mode is more effective in reducing the heat source temperature and achieving better system performance. Compared to the conventional TCES mode, the proposed modes can operate at lower heat source temperatures that can be minimized by up to 21∼25°C by employing the B mode with a compression ratio of 3.0 at the condensing temperature of 24°C. The B mode with SrBr2·H2O/H2O exhibits the highest energy and exergy efficiencies that the coefficients of performance based on total energy input and electric power consumed (COPtotal and COPelec), and exergy efficiency varies in the range of 0.53∼0.59, 7.4∼19.6, and 0.78∼0.95, respectively. In contrast, CaCl2·H2O/H2O shows the lowest system performance, but a higher heat output temperature can be required. In addition, to maintain the MBP discharge temperature below 180°C, there is a maximum permitted compression ratio that varies depending on the operating modes, operating conditions, and working pairs. The findings of this research can be used as theoretical references and suggestions for selecting MBP-assisted TCES modes, operating conditions, and working pairs for low-grade heat storage and upgrading.
Simulation Study on Lightning Impulse Characteristics of Flexible Graphite Composite Grounding Materials Applied to Grounding Grid of Power System
Authors: Hu Yuanchao , Huang Tao , An Yunzhu , Feng Jianyuan , Cheng Meng , Xie Hongping , Shen Wentao , Du Changqing
Published: March 2022

The flexible graphite composite grounding electrode is a non-metallic grounding electrode with good electrical conductivity, corrosion resistance and non-ferromagnetic properties. In order to analyze the impulse characteristics of the graphite composite grounding electrode, this paper builds a frequency domain electrical network model and an equivalent radius iterative algorithm, considering skin effect, inductance effect, capacitance effect and spark discharge effect. The impulse characteristics of typical metal grounding electrodes and graphite composite grounding electrodes are analyzed by simulation. The research results show that: Compared with the traditional metal grounding electrode, the graphite composite grounding electrode has a smaller skin and inductance effect under the action of the impulse current, and a better current flowing capability; as the soil resistivity increases, the inductance effect and the skin effect are weakened, while the spark discharge effect is gradually enhanced and dominates. The spark discharge effect can effectively decrease the grounding resistance. The obtained critical value of the normalized parameter of 412 kA Ω, can be taken as the threshold to discriminate the conditions with dominate inductance effect from the conditions with dominant spark discharge effect.
Monsoon Fringe Area Moisture Transportation Revealed by Water Stable Isotopes: A Lanzhou River Valley Case Study
Authors: Du Jia , Liu Jingfeng , Zhang Mingjun
Published: March 2022

Because of global warming, the water cycle has intensified and precipitation has changed, with monsoons evolving to become more complex, particularly in monsoon fringe areas. Therefore, it is important to use stable isotopes to trace moisture sources in monsoon fringe areas, such as semiarid areas. In this study, in situ measurements of water vapor isotopes were obtained in the monsoon fringe areas of Lanzhou (36.1° N, 103.7° E), between May and November 2019 with the aim of analyzing the stable isotope composition and atmospheric water vapor variation to improve our understanding of moisture transport. The following results were obtained: stable isotopic variation of atmospheric water vapor shows an alternation between west and southwest moisture with wind directions and sources of water vapor, with δ18O and δ2H varying from high to low. In the Lanzhou valley, water vapor isotopes show a distinct daily cycle of δ17O, δ18O, and δ2H and are consistent with temperature changes. Water vapor isotopes, particularly d-excess, are correlated with temperature and absolute humidity. With regard to the local atmospheric water vapor line (LAVL), we show that the slope and intercept of the LAVL are affected by sub-cloud secondary evaporation and topography.
Indoleamine 2, 3-Dioxygenase 1 Mediates Survival Signals in Chronic Lymphocytic Leukemia via Kynurenine/Aryl Hydrocarbon Receptor-Mediated MCL1 Modulation
Authors: Atene Claudio Giacinto , Fiorcari Stefania , Mesini Nicolò , Alboni Silvia , Martinelli Silvia , Maccaferri Monica , Leonardi Giovanna , Potenza Leonardo , Luppi Mario , Maffei Rossana , Marasca Roberto
Published: March 2022

The indoleamine 2,3-dioxygenase 1 (IDO1) metabolic circuitry, comprising the first tryptophan (Trp) catabolite L-kynurenine (Kyn) and the aryl hydrocarbon receptor (AHR), has emerged as a mechanism of cancer immune evasion. Here, we investigated the functional role of the IDO1/Kyn/AHR axis in chronic lymphocytic leukemia (CLL). Our data show that CLL cells expressed an active form of the IDO1 enzyme and microenvironmental stimuli can positively modulate its expression. Interferon (IFN)-γ induces IDO1 expression through the Jak/STAT1 pathway and mediates Kyn production concomitantly with Trp consumption in CLL-conditioned media, while INCB018424 (ruxolitinib), a JAK1/2 inhibitor, impaired both effects. To characterize the involvement of IDO1 in leukemic cell maintenance, we overexpressed IDO1 by vector transfection measuring enhanced resistance to spontaneous apoptosis. IDO1 pro-survival influence was confirmed by treating CLL cells with Kyn, which mediated the increase of induced myeloid leukemia cell differentiation protein (MCL1). Conversely, AHR silencing or its blockade via CH-223191 improved the apoptosis of leukemic clones and mitigated MCL1 expression. Moreover, Kyn-treated CLL cells are less affected by the pro-apoptotic effect of ABT-199 (venetoclax), while CH-223191 showed synergistic/additive cytotoxicity with this drug. Lastly, targeting directly MCL1 in CLL cells with AMG-176, we abrogate the pro-survival effect of Kyn. In conclusion, our data identify IDO1/Kyn/AHR signaling as a new therapeutic target for CLL, describing for the first time its role in CLL pathobiology.
Alcohol Consumption and Risk of Liver Fibrosis in People Living With HIV: A Systematic Review and Meta-Analysis
Authors: Lyu Hang , Tang Haotong , Liang Yizhi , Huang Shaoli , Wang Yuyu , Huang Wenyan , Zhou Yi
Published: March 2022

Objectives

It is unclear if a high level of alcohol consumption is a risk factor for liver fibrosis for people living with HIV (PLWH). This study systematically summarizes the risk relationship between different alcohol consumption and the incidence of liver fibrosis among PLWH.

Methods

We identified potential studies by searching the PubMed, Embase, Web of Science Library, and CNKI databases up to September 26th, 2021. Observation studies in PLWH that evaluated the relationship between alcohol consumption and the risk of liver fibrosis and estimated the effect of alcohol with pooled odds ratios (pooled ORs) and 95% confidence intervals (CIs) were included.

Results

There were total 15 studies included in data analysis. Three studies were set up as cohort studies and the other twelve were cross-sectional studies. Our study was based on 22,676 individuals and 2,729 liver fibrosis cases from 15 studies. Alcohol abuse is a significant risk factor of liver fibrosis (pooled OR = 2.25, 95% CI: 1.59-3.17, p < 0.05) among PLWH. Daily alcohol consumption > 50 g can elevate the risk of liver fibrosis (pooled OR = 3.10, 95% CI: 2.02-4.73, p < 0.05) among PLWH. However, high-risk alcohol consumption determined by AUDIT-C (AUDIT-C ≥ 4) had little or no effect on subsequent liver fibrosis risk. Further, alcohol consumption > 50 g is also a risk factor to liver fibrosis in PLWH co-infected with HCV (pooled OR = 2.48, 95% CI: 1.62-3.80, p < 0.05) and in HIV mono-infected (pooled OR = 1.85, 95% CI: 1.00-3.43, p < 0.05).

Conclusion

Alcohol consumption is associated with an increased risk of liver fibrosis in PLWH. HCV co-infection with alcohol abuse could possibly induce a higher risk of liver fibrosis than HIV mono-infected patients.

Systematic Review Registration

PROSPERO, identifier (CRD42021272604).
Oncogenic and Tumor Suppressor Functions for Lymphoid Enhancer Factor 1 in E2a-/- T Acute Lymphoblastic Leukemia
Authors: Carr Tiffany , McGregor Stephanie , Dias Sheila , Verykokakis Mihalis , Le Beau Michelle M. , Xue Hai-Hui , Sigvardsson Mikael , Bartom Elizabeth T. , Kee Barbara L.
Published: March 2022

T lymphocyte acute lymphoblastic leukemia (T-ALL) is a heterogeneous disease affecting T cells at multiple stages of their development and is characterized by frequent genomic alterations. The transcription factor LEF1 is inactivated through mutation in a subset of T-ALL cases but elevated LEF1 expression and activating mutations have also been identified in this disease. Here we show, in a murine model of T-ALL arising due to E2a inactivation, that the developmental timing of Lef1 mutation impacts its ability to function as a cooperative tumor suppressor or oncogene. T cell transformation in the presence of LEF1 allows leukemic cells to become addicted to its presence. In contrast, deletion prior to transformation both accelerates leukemogenesis and results in leukemic cells with altered expression of genes controlling receptor-signaling pathways. Our data demonstrate that the developmental timing of Lef1 mutations impact its apparent oncogenic or tumor suppressive characteristics and demonstrate the utility of mouse models for understanding the cooperation and consequence of mutational order in leukemogenesis.
Synovial Fibroblast Sialylation Regulates Cell Migration and Activation of Inflammatory Pathways in Arthritogenesis
Authors: Wang Yilin , Pan Piaopiao , Khan Aneesah , Çil Çağlar , Pineda Miguel A.
Published: March 2022

Synovial fibroblasts have emerged as critical underlying factors to perpetuate chronic joint inflammation in Rheumatoid Arthritis. Like any other cell, synovial fibroblasts are covered with a complex layer of glycans that can change in response to extracellular signals, such as inflammation. We have previously shown that inflammatory synovial fibroblasts show decreased levels of sialic acid, but our understanding of sialic acid-dependent pathophysiological pathways in these stromal cells is still very limited. In this report, we used in vivo and in vitro studies with exogenous sialidases and RNA sequencing to investigate the responses of murine synovial fibroblasts upon desialylation. Our results show that hyposialylated fibroblasts present a dysregulated migratory ability and an activated phenotype characterized by the expression of inflammatory mediators, such as cytokines and chemokines, and anti-viral related mechanisms. Removal of surface sialic acid also affected the expression of sialyltransferases, revealing the existence of a positive feedback to sustain reduced sialylation. Moreover, we demonstrate that synovial fibroblasts subsets have distinct sialyltransferase expression profiles, both in healthy and arthritic mice. These findings underline the ability of sialic acid to modulate homeostatic and inflammatory responses in non-immune synovial fibroblasts, suggesting that sialylation plays a key role in perpetuating local inflammation in the arthritic joint.
Normal Light-Dark and Short-Light Cycles Regulate Intestinal Inflammation, Circulating Short-chain Fatty Acids and Gut Microbiota in Period2 Gene Knockout Mice
Authors: Zhen Yongkang , Ge Ling , Xu Qiaoyun , Hu Liangyu , Wei Wenjun , Huang Jiantao , Loor Juan J. , Yang Qingyong , Wang Mengzhi , Zhou Ping
Published: March 2022

Regular environmental light–dark (LD) cycle-regulated period circadian clock 2 (Per2) gene expression is essential for circadian oscillation, nutrient metabolism, and intestinal microbiota balance. Herein, we combined environmental LD cycles with Per2 gene knockout to investigate how LD cycles mediate Per2 expression to regulate colonic and cecal inflammatory and barrier functions, microbiome, and short-chain fatty acids (SCFAs) in the circulation. Mice were divided into knockout (KO) and wild type (CON) under normal light–dark cycle (NLD) and short-light (SL) cycle for 2 weeks after 4 weeks of adaptation. The concentrations of SCFAs in the serum and large intestine, the colonic and cecal epithelial circadian rhythm, SCFAs transporter, inflammatory and barrier-related genes, and Illumina 16S rRNA sequencing were measured after euthanasia during 10:00–12:00. KO decreased the feeding frequency at 0:00–2:00 but increased at 12:00–14:00 both under NLD and SL. KO upregulated the expression of Per1 and Rev-erbα in the colon and cecum, while it downregulated Clock and Bmal1. In terms of inflammatory and barrier functions, KO increased the expression of Tnf-α, Tlr2, and Nf-κb p65 in the colon and cecum, while it decreased Claudin and Occludin-1. KO decreased the concentrations of total SCFAs and acetate in the colon and cecum, but it increased butyrate, while it had no impact on SCFAs in the serum. KO increased the SCFAs transporter because of the upregulation of Nhe1, Nhe3, and Mct4. Sequencing data revealed that KO improved bacteria α-diversity and increased Lachnospiraceae and Ruminococcaceae abundance, while it downregulated Erysipelatoclostridium, Prevotellaceae UCG_001, Olsenella, and Christensenellaceae R-7 under NLD in KO mice. Most of the differential bacterial genus were enriched in amino acid and carbohydrate metabolism pathways. Overall, Per2 knockout altered circadian oscillation in the large intestine, KO improved intestinal microbiota diversity, the increase in Clostridiales abundance led to the reduction in SCFAs in the circulation, concentrations of total SCFAs and acetate decreased, while butyrate increased and SCFAs transport was enhanced. These alterations may potentially lead to inflammation of the large intestine. Short-light treatment had minor impact on intestinal microbiome and metabolism.
Lipid Metabolism: Immune Regulation and Therapeutic Prospectives in Systemic Lupus Erythematosus
Authors: Sun Wei , Li Pengchong , Cai Jianping , Ma Jie , Zhang Xuan , Song Yong , Liu Yudong
Published: March 2022

Systemic lupus erythematosus (SLE) is a heterogeneous disease characterized by the production of abnormal autoantibodies and immune complexes that can affect the organ and organ systems, particularly the kidneys and the cardiovascular system. Emerging evidence suggests that dysregulated lipid metabolism, especially in key effector cells, such as T cells, B cells, and innate immune cells, exerts complex effects on the pathogenesis and progression of SLE. Beyond their important roles as membrane components and energy storage, different lipids can also modulate different cellular processes, such as proliferation, differentiation, and survival. In this review, we summarize altered lipid metabolism and the associated mechanisms involved in the pathogenesis and progression of SLE. Furthermore, we discuss the recent progress in the role of lipid metabolism as a potential therapeutic target in SLE.
The Antenna Base Plays a Crucial Role in Mosquito Courtship Behavior
Authors: Ziemer Tim , Wetjen Fabian , Herbst Alexander
Published: March 2022

Mosquitoes are vectors of pathogens that cause diseases like malaria, dengue fever, yellow fever, chikungunya and Zika. For mosquito control it is crucial to understand their hearing system, as mosquitoes’ courting behavior is mostly auditory. Many nonlinear characteristics of the mosquito hearing organ have been observed through behavioral studies and neural measurements. These enable mosquitoes to detect and synchronize to other mosquitoes. Many hypotheses concerning the role of the flagellum and the fibrillae of the antenna in mosquito hearing have been made, and neural processes have been considered as the origin of the nonlinearities. In this study we introduce a geometric model based on the morphology of the mosquito antenna base. The model produces many of the observed nonlinear characteristics, providing evidence that the base of the antenna plays a crucial role in mosquito hearing. Even without neural processing, the antenna response to sound produces behaviorally relevant cues that can inform about the presence, location, and sex of other mosquitoes.
The EMSO Generic Instrument Module (EGIM): Standardized and Interoperable Instrumentation for Ocean Observation
Authors: Lantéri Nadine , Ruhl Henry A. , Gates Andrew , Martínez Enoc , del Rio Fernandez Joaquin , Aguzzi Jacopo , Cannat Mathilde , Delory Eric , Embriaco Davide , Huber Robert , Matabos Marjolaine , Petihakis George , Reilly Kieran , Rolin Jean-François , van der Schaar Mike , André Michel , Blandin Jérôme , Cianca Andrés , Francescangeli Marco , Garcia Oscar , Hartman Susan , Lagadec Jean-Romain , Legrand Julien , Pagonis Paris , Piera Jaume , Remirez Xabier , Toma Daniel M. , Marinaro Giuditta , Moreau Bertrand , Santana Raul , Wright Hannah , Dañobeitia Juan José , Favali Paolo
Published: March 2022

The oceans are a fundamental source for climate balance, sustainability of resources and life on Earth, therefore society has a strong and pressing interest in maintaining and, where possible, restoring the health of the marine ecosystems. Effective, integrated ocean observation is key to suggesting actions to reduce anthropogenic impact from coastal to deep-sea environments and address the main challenges of the 21st century, which are summarized in the UN Sustainable Development Goals and Blue Growth strategies. The European Multidisciplinary Seafloor and water column Observatory (EMSO), is a European Research Infrastructure Consortium (ERIC), with the aim of providing long-term observations via fixed-point ocean observatories in key environmental locations across European seas from the Arctic to the Black Sea. These may be supported by ship-based observations and autonomous systems such as gliders. In this paper, we present the EMSO Generic Instrument Module (EGIM), a deployment ready multi-sensor instrumentation module, designed to measure physical, biogeochemical, biological and ecosystem variables consistently, in a range of marine environments, over long periods of time. Here, we describe the system, features, configuration, operation and data management. We demonstrate, through a series of coastal and oceanic pilot experiments that the EGIM is a valuable standard ocean observation module, which can significantly improve the capacity of existing ocean observatories and provides the basis for new observatories. The diverse examples of use included the monitoring of fish activity response upon oceanographic variability, hydrothermal vent fluids and particle dispersion, passive acoustic monitoring of marine mammals and time series of environmental variation in the water column. With the EGIM available to all the EMSO Regional Facilities, EMSO will be reaching a milestone in standardization and interoperability, marking a key capability advancement in addressing issues of sustainability in resource and habitat management of the oceans.
A Novel Prediction Model for Brain Glioma Image Segmentation Based on the Theory of Bose-Einstein Condensate
Authors: Zhang Tian Chi , Zhang Jing , Chen Shou Cun , Saada Bacem
Published: March 2022

Background

The input image of a blurry glioma image segmentation is, usually, very unclear. It is difficult to obtain the accurate contour line of image segmentation. The main challenge facing the researchers is to correctly determine the area where the points on the contour line belong to the glioma image. This article highlights the mechanism of formation of glioma and provides an image segmentation prediction model to assist in the accurate division of glioma contour points. The proposed prediction model of segmentation associated with the process of the formation of glioma is innovative and challenging. Bose-Einstein Condensate (BEC) is a microscopic quantum phenomenon in which atoms condense to the ground state of energy as the temperature approaches absolute zero. In this article, we propose a BEC kernel function and a novel prediction model based on the BEC kernel to detect the relationship between the process of the BEC and the formation of a brain glioma. Furthermore, the theoretical derivation and proof of the prediction model are given from micro to macro through quantum mechanics, wave, oscillation of glioma, and statistical distribution of laws. The prediction model is a distinct segmentation model that is guided by BEC theory for blurry glioma image segmentation.

Results

Our approach is based on five tests. The first three tests aimed at confirming the measuring range of T and μ in the BEC kernel. The results are extended from −10 to 10, approximating the standard range to T ≤ 0, and μ from 0 to 6.7. Tests 4 and 5 are comparison tests. The comparison in Test 4 was based on various established cluster methods. The results show that our prediction model in image evaluation parameters of P, R, and F is the best amongst all the existent ten forms except for only one reference with the mean value of F that is between 0.88 and 0.93, while our approach returns between 0.85 and 0.99. Test 5 aimed to further compare our results, especially with CNN (Convolutional Neural Networks) methods, by challenging Brain Tumor Segmentation (BraTS) and clinic patient datasets. Our results were also better than all reference tests. In addition, the proposed prediction model with the BEC kernel is feasible and has a comparative validity in glioma image segmentation.

Conclusions

Theoretical derivation and experimental verification show that the prediction model based on the BEC kernel can solve the problem of accurate segmentation of blurry glioma images. It demonstrates that the BEC kernel is a more feasible, valid, and accurate approach than a lot of the recent year segmentation methods. It is also an advanced and innovative model of prediction deducing from micro BEC theory to macro glioma image segmentation.
The Association of New-Onset Acute Kidney Injury and Mortality in Critically Ill Patients With COVID-19 With Less Severe Clinical Conditions at Admission: A Moderation Analysis
Authors: Regolisti Giuseppe , Maggiore Umberto , Di Mario Francesca , Gentile Micaela , Benigno Giuseppe Daniele , Gandolfini Ilaria , Pistolesi Valentina , Morabito Santo , Barbagallo Maria , Picetti Edoardo , Fiaccadori Enrico
Published: March 2022

Acute kidney injury (AKI), electrolyte, and acid–base disorders complicate the clinical course of critically ill patients with coronavirus-associated disease (COVID-19) and are associated with poor outcomes. It is not known whether the severity of clinical conditions at admission in the intensive care unit (ICU) changes the clinical significance of AKI and/or electrolyte or acid–base disorders developing during ICU stay. We conducted a retrospective study in critically ill patients with COVID-19 to evaluate whether the severity of clinical conditions at admission in the ICU affects the impact of AKI and of serum electrolytes or acid–base status on mortality. We carried out a 28-day retrospective follow-up study on 115 critically ill patients consecutively admitted to ICU for severe COVID-19 at a tertiary care university hospital and surviving longer than 24 h. We collected baseline demographic and clinical characteristics, and longitudinal data on kidney function, kidney replacement therapy, serum electrolytes, and acid–base status. We used Cox proportional hazards multiple regression models to test the interaction between the time-varying variates new-onset AKI or electrolyte or acid–base disorders and Sequential Organ Failure Assessment (SOFA) or Acute Physiology and Chronic Health Evaluation II (APACHE II) score at admission. After adjusting for age, sex, Charlson’s comorbidity index, and AKI present at ICU admission, new-onset AKI was significantly associated with 28-day mortality only in the patients in the lowest and middle SOFA score tertiles [lowest SOFA tertile, hazard ratio (HR) 4.27 (95% CI: 1.27–14.44; P = 0.019), middle SOFA tertile, HR 3.17 (95% CI: 1.11–9.04, P = 0.031), highest SOFA tertile, HR 0.77 (95% CI: 0.24–2.50; P = 0.66); P = 0.026 for interaction with SOFA as a continuous variable]. After stratifying for APACHE II tertile, results were similar [adjusted HR (aHR) in the lowest tertile 6.24 (95% CI: 1.85–21.03, P = 0.003)]. SOFA or APACHE II at admission did not affect the relationship of serum electrolytes and acid–base status with mortality, except for new-onset acidosis which was associated with increased mortality, with the HR of death increasing with SOFA or APACHE II score (P < 0.001 and P = 0.013, respectively). Thus, unlike in the most severe critically ill patients admitted to the ICU for COVID-19, in patients with the less severe conditions at admission the development of AKI during the stay is a strong indicator of increased hazard of death.
Prediction of MYCN Amplification, 1p and 11q Aberrations in Pediatric Neuroblastoma via Pre-therapy 18F-FDG PET/CT Radiomics
Authors: Qian Luodan , Yang Shen , Zhang Shuxin , Qin Hong , Wang Wei , Kan Ying , Liu Lei , Li Jixia , Zhang Hui , Yang Jigang
Published: March 2022

Purpose

This study aimed to assess the predictive ability of 18F-FDG PET/CT radiomic features for MYCN, 1p and 11q abnormalities in NB.

Method

One hundred and twenty-two pediatric patients (median age 3. 2 years, range, 0.2–9.8 years) with NB were retrospectively enrolled. Significant features by multivariable logistic regression were retained to establish a clinical model (C_model), which included clinical characteristics. 18F-FDG PET/CT radiomic features were extracted by Computational Environment for Radiological Research. The least absolute shrinkage and selection operator (LASSO) regression was used to select radiomic features and build models (R-model). The predictive performance of models constructed by clinical characteristic (C_model), radiomic signature (R_model), and their combinations (CR_model) were compared using receiver operating curves (ROCs). Nomograms based on the radiomic score (rad-score) and clinical parameters were developed.

Results

The patients were classified into a training set (n = 86) and a test set (n = 36). Accordingly, 6, 8, and 7 radiomic features were selected to establish R_models for predicting MYCN, 1p and 11q status. The R_models showed a strong power for identifying these aberrations, with area under ROC curves (AUCs) of 0.96, 0.89, and 0.89 in the training set and 0.92, 0.85, and 0.84 in the test set. When combining clinical characteristics and radiomic signature, the AUCs increased to 0.98, 0.91, and 0.93 in the training set and 0.96, 0.88, and 0.89 in the test set. The CR_models had the greatest performance for MYCN, 1p and 11q predictions (P < 0.05).

Conclusions

The pre-therapy 18F-FDG PET/CT radiomics is able to predict MYCN amplification and 1p and 11 aberrations in pediatric NB, thus aiding tumor stage, risk stratification and disease management in the clinical practice.
Genetic Characteristics of Porcine Hemagglutinating Encephalomyelitis Coronavirus: Identification of Naturally Occurring Mutations Between 1970 and 2015
Authors: Bahoussi Amina Nawal , Guo Yan-Yan , Shi Rui-Zhu , Wang Pei-Hua , Li Ya-Qian , Wu Chang-Xin , Xing Li
Published: March 2022

Porcine hemagglutinating encephalomyelitis virus (PHEV) is a Betacoronavirus characterized by neurological symptoms and a worldwide prevalence. Although PHEV is one of the earliest discovered porcine coronaviruses, it remains poorly studied. The full-length genome of the earliest PHEV strain collected in 1970 in the United States (PHEV/67 N/US/1970) was determined in October 2020. Using this virus as a prototype, we comparatively analyzed all available PHEV full-length sequences during 1970–2015. In phylogenetic trees based on PHEV full-length or spike glycoprotein open reading frame genomic sequences, PHEV/67 N/US/1970 was sorted into a clade different from that of viruses isolated in the United States in 2015. Intriguingly, United States and Belgium viruses isolated in 2015 and 2005, respectively, revealed multiple deletion mutation patterns compared to the strain PHEV/67 N/US/1970, leading to a truncated or a non-functional NS2A coding region. In addition, the genomic similarity analysis showed a hypervariability of the spike glycoprotein coding region, which can affect at least eight potential linear B cell epitopes located in the spike glycoprotein. This report indicates that PHEVs in the United States underwent a significant genetic drift, which might influence PHEV surveillance in other countries.
Targeting the Pseudomonas aeruginosa Virulence Factor Phospholipase C With Engineered Liposomes
Authors: Wolfmeier Heidi , Wardell Samuel J. T. , Liu Leo T. , Falsafi Reza , Draeger Annette , Babiychuk Eduard B. , Pletzer Daniel , Hancock Robert E. W.
Published: March 2022

Engineered liposomes composed of the naturally occurring lipids sphingomyelin (Sm) and cholesterol (Ch) have been demonstrated to efficiently neutralize toxins secreted by Gram-positive bacteria such as Streptococcus pneumoniae and Staphylococcus aureus. Here, we hypothesized that liposomes are capable of neutralizing cytolytic virulence factors secreted by the Gram-negative pathogen Pseudomonas aeruginosa. We used the highly virulent cystic fibrosis P. aeruginosa Liverpool Epidemic Strain LESB58 and showed that sphingomyelin (Sm) and a combination of sphingomyelin with cholesterol (Ch:Sm; 66 mol/% Ch and 34 mol/% Sm) liposomes reduced lysis of human bronchial and red blood cells upon challenge with the Pseudomonas secretome. Mass spectrometry of liposome-sequestered Pseudomonas proteins identified the virulence-promoting hemolytic phospholipase C (PlcH) as having been neutralized. Pseudomonas aeruginosa supernatants incubated with liposomes demonstrated reduced PlcH activity as assessed by the p-nitrophenylphosphorylcholine (NPPC) assay. Testing the in vivo efficacy of the liposomes in a murine cutaneous abscess model revealed that Sm and Ch:Sm, as single dose treatments, attenuated abscesses by >30%, demonstrating a similar effect to that of a mutant lacking plcH in this infection model. Thus, sphingomyelin-containing liposome therapy offers an interesting approach to treat and reduce virulence of complex infections caused by P. aeruginosa and potentially other Gram-negative pathogens expressing PlcH.
Brain Changes Associated With Long-Term Ketamine Abuse, A Systematic Review
Authors: Strous Jurriaan F. M. , Weeland Cees J. , van der Draai Femke A. , Daams Joost G. , Denys Damiaan , Lok Anja , Schoevers Robert A. , Figee Martijn
Published: March 2022

Recently, the abuse of ketamine has soared. Therefore, it is of great importance to study its potential risks. The effects of prolonged ketamine on the brain can be observationally studied in chronic recreational users. We performed a systematic review of studies reporting functional and structural brain changes after repeated ketamine abuse. We searched the following electronic databases: Medline, Embase and PsycINFO We screened 11,438 records and 16 met inclusion criteria, totaling 440 chronic recreational ketamine users (2–9.7 years; mean use 2.4 g/day), 259 drug-free controls and 44 poly-drug controls. Long-term recreational ketamine use was associated with lower gray matter volume and less white matter integrity, lower functional thalamocortical and corticocortical connectivity. The observed differences in both structural and functional neuroanatomy between ketamine users and controls may explain some of its long-term cognitive and psychiatric side effects, such as memory impairment and executive functioning. Given the effect that long-term ketamine exposure may yield, an effort should be made to curb its abuse.
Clinical, Radiological, Pathological Features and Seizure Outcome With Surgical Management of Polymorphous Low-Grade Neuroepithelial Tumor of the Young Associated With Epilepsy
Authors: Fei Xiaorui , Zhao Jing , Wei Wei , Wang Wei , Kong Xue , Qian Ruobing , Niu Chaoshi , Yao Yang
Published: March 2022

Objective

Polymorphous low-grade neuroepithelial tumor of the young (PLNTY) is a novel distinct epileptogenic neoplasm, and its clinical, imaging, histopathological, and molecular features were already known in the existing literature. We aimed to analyze the surgical management of PLNTY combined with these known characteristics.

Methods

Eight patients underwent surgical treatment in our center between December 2017 and December 2020, and the postoperative pathology was diagnosed as PLNTY. Their clinical data, imaging, pathological, molecular characteristics, and seizure outcome were retrospectively analyzed. Follow-up evaluations and a literature review were performed.

Results

The 8 patients included 1 woman and 7 men, aged between 5 and 51 years old (mean = 31.6, median = 29). The preoperative symptoms of all 8 cases were seizures. Four tumors were situated in the temporal lobes, and one of the four extratemporal tumors was in the occipital lobe and three were in the frontal lobe. Enlarged and gross total resections were performed in 2 cases and the other 6 cases, respectively. All cases exhibited intense labeling of CD34, and absence of 1p/19q codeletion and IDH1 or IDH2 mutation. B-Raf proto-oncogene (BRAF) V600E mutation was presented in 4 (66.7%) of 6 detected cases. The postoperative seizure outcome of Engel class I was achieved in 6 cases (75%).

Conclusion

PLNTY represents distinctive histologic, immunophenotypic and biomolecular features, and has high epileptogenicity. Early surgical intervention and enlarged resection of PLNTY associated with epilepsy will help to improve the postoperative seizure-free rate.
Venovenous vs. Venoarterial Extracorporeal Membrane Oxygenation in Infection-Associated Severe Pediatric Acute Respiratory Distress Syndrome: A Prospective Multicenter Cohort Study
Authors: Cui Yun , Zhang Yucai , Dou Jiaying , Shi Jingyi , Zhao Zhe , Zhang Zhen , Chen Yingfu , Cheng Chao , Zhu Desheng , Quan Xueli , Zhu Xuemei , Huang Wenyan
Published: March 2022

Background

Extracorporeal membrane oxygenation (ECMO) has been increasingly used as rescue therapy for severe pediatric acute respiratory distress syndrome (PARDS) over the past decade. However, a contemporary comparison of venovenous (VV) and venoarterial (VA) ECMO in PARDS has yet to be well described. Therefore, the objective of our study was to assess the difference between VV and VA ECMO in efficacy and safety for infection-associated severe PARDS patients.

Methods

This prospective multicenter cohort study included patients with infection-associated severe PARDS who received VV or VA ECMO in pediatric intensive care units (PICUs) of eight university hospitals in China between December 2018 to June 2021. The primary outcome was in-hospital mortality. Secondary outcomes included ECMO weaning rate, duration of ECMO and mechanical ventilation (MV), ECMO-related complications, and hospitalization costs.

Results

A total of 94 patients with 26 (27.66%) VV ECMO and 68 (72.34%) VA ECMO were enrolled. Compared to the VA ECMO patients, VV ECMO patients displayed a significantly lower in-hospital mortality (50 vs. 26.92%, p = 0.044) and proportion of neurologic complications, shorter duration of ECMO and MV, but the rate of successfully weaned from ECMO, bleeding, bloodstream infection complications and pump failure were similar. By contrast, oxygenator failure was more frequent in patients receiving VV ECMO. No significant intergroup difference was observed for the hospitalization costs.

Conclusion

These positive findings showed the conferred survival advantage and safety of VV ECMO compared with VA ECMO, suggesting that VV ECMO may be an effective initial treatment for patients with infection-associated severe PARDS.