bluuebunny/crossref_metadata_2025 huggingface dataset:
Bone Regeneration: A Novel Osteoinductive Function of Spongostan by the Interplay between Its Nano- and Microtopography
Authors: Vordemvenne Thomas , Wähnert Dirk , Koettnitz Julian , Merten Madlen , Fokin Nadine , Becker Andreas , Büker Björn , Vogel Asaria , Kronenberg Daniel , Stange Richard , Wittenberg Günther , Greiner Johannes FW , Hütten Andreas , Kaltschmidt Christian , Kaltschmidt Barbara
Published: March 2020

Scaffold materials for bone regeneration are crucial for supporting endogenous healing after accidents, infections, or tumor resection. Although beneficial impacts of microtopological or nanotopological cues in scaffold topography are commonly acknowledged, less consideration is given to the interplay between the microscale and nanoscale. Here, micropores with a 60.66 ± 24.48 µm diameter ordered by closely packed collagen fibers are identified in pre-wetted Spongostan, a clinically-approved collagen sponge. On a nanoscale level, a corrugated surface of the collagen sponge is observable, leading to the presence of 32.97 ± 1.41 nm pores. This distinct micro- and nanotopography is shown to be solely sufficient for guiding osteogenic differentiation of human stem cells in vitro. Transplantation of Spongostan into a critical-size calvarial rat bone defect further leads to fast regeneration of the lesion. However, masking the micro- and nanotopographical cues using SiO2 nanoparticles prevents bone regeneration in vivo. Therefore, we demonstrate that the identified micropores allow migration of stem cells, which are further driven towards osteogenic differentiation by scaffold nanotopography. The present findings emphasize the necessity of considering both micro- and nanotopographical cues to guide intramembranous ossification, and might provide an optimal cell- and growth-factor-free scaffold for bone regeneration in clinical settings.
Vascular Smooth Muscle Cell-Specific Progerin Expression Provokes Contractile Impairment in a Mouse Model of Hutchinson-Gilford Progeria Syndrome that Is Ameliorated by Nitrite Treatment
Authors: del Campo Lara , Sánchez-López Amanda , González-Gómez Cristina , Andrés-Manzano María Jesús , Dorado Beatriz , Andrés Vicente
Published: March 2020

Cardiovascular disease (CVD) is the main cause of death worldwide, and aging is its leading risk factor. Aging is much accelerated in Hutchinson–Gilford progeria syndrome (HGPS), an ultra-rare genetic disorder provoked by the ubiquitous expression of a mutant protein called progerin. HGPS patients die in their teens, primarily due to cardiovascular complications. The primary causes of age-associated CVD are endothelial dysfunction and dysregulated vascular tone; however, their contribution to progerin-induced CVD remains poorly characterized. In the present study, we found that progeroid LmnaG609G/G609G mice with ubiquitous progerin expression show both endothelial dysfunction and severe contractile impairment. To assess the relative contribution of specific vascular cell types to these anomalies, we examined LmnaLCS/LCSTie2Cretg/+ and LmnaLCS/LCSSm22αCretg/+ mice, which express progerin specifically in endothelial cells (ECs) and vascular smooth muscle cells (VSMCs), respectively. Whereas vessel contraction was impaired in mice with VSMC-specific progerin expression, we observed no endothelial dysfunction in mice with progerin expression restricted to VSMCs or ECs. Vascular tone regulation in progeroid mice was ameliorated by dietary sodium nitrite supplementation. Our results identify VSMCs as the main cell type causing contractile impairment in a mouse model of HGPS that is ameliorated by nitrite treatment.
Plasma Diagnostics in Reactive High-Power Impulse Magnetron Sputtering System Working in Ar + H2S Gas Mixture
Authors: Hubička Z. , Čada M. , Kapran A. , Olejníček J. , Kšírová P. , Zanáška M. , Adámek P. , Tichý M.
Published: March 2020

A reactive high-power impulse magnetron sputtering system (HiPIMS) working in Ar + H2S gas mixture was investigated as a source for the deposition of iron sulfide thin films. As a sputtering material, a pure Fe target was used. Plasma parameters in this system were investigated by a time-resolved Langmuir probe, radio-frequency (RF) ion flux probe, quartz crystal monitor modified for measurement of the ionized fraction of depositing particles, and by optical emission spectroscopy. A wide range of mass flow rates of reactive gas H2S was used for the investigation of the deposition process. It was found that the deposition rate of iron sulfide thin films is not influenced by the flow rate of H2S reactive gas fed into the magnetron discharge although the target is covered by iron sulfide compound. The ionized fraction of depositing particles decreases from r ≈ 40% to r ≈ 20% as the flow rate of H2S, QH2S, changes from 0 to 19 sccm at the gas pressure around p ≈ 1 Pa in the reactor chamber. The electron concentration ne measured by the Langmuir probe at the position of the substrate decreases over this change of QH2S from 1018 down to 1017 m−3
Plasma Treatment Reduced the Discoloration of an Acrylic Coating on Hot-Oil Modified Wood Exposed to Natural Weathering
Authors: Jamali Arash , Evans Philip D.
Published: March 2020

We test the hypothesis that plasma-treatment will remove oil from the surface of hot-oil modified blue-stained pine wood, and improve the adhesion and outdoor performance of a white acrylic coating on the modified wood. Modified wood was treated with water-vapour plasma, and microstructural changes at wood surfaces were examined. Plasma treatment removed oil from the surface of modified wood and etched bordered pits. The contact angle of water droplets on modified wood was 91.8°, but plasma-treatment for only 33 s reduced contact angle to less than that of the unmodified control (48.6°). The adhesion of the acrylic paint to modified wood was unaffected by plasma-treatment, but the adhesion rating of coated samples tested wet was slightly lower (3.1) than that of the coating on samples tested dry (3.5). The lightness value (CIE-L) of the acrylic coating on hot-oil modified wood samples exposed outdoors for 18 months was significantly lower (darker, 65.5) than that of the coating on similarly modified and exposed samples pre-treated with plasma (75.8). We conclude that plasma-treatment shows promise as a way of removing oil from the surface of hot-oil modified wood and reducing the discolouration of an acrylic coating on modified wood exposed to natural weathering.
Corrosion and Wear Behavior of PEO Coatings on D16T Aluminum Alloy with Different Concentrations of Graphene
Authors: Liu Wanying , Pu Yi , Liao Hongcheng , Lin Yuanhua , He Wanying
Published: March 2020

The effect of added graphene concentration on the microstructure, phase composition, corrosion- and wear- resistance of plasma electrolyte oxidation (PEO) coatings formed on D16T aluminum alloy in silicate electrolyte with different concentrations of graphene were investigated. The results show that the morphologies of the coatings with graphene were obviously different ascribed to the mode of graphene incorporated into the coating. The coatings consisted of mainly α-Al2O3, γ-Al2O3, and Al, which were divided into an outer porous layer and a dense inner layer. The thickness of the coatings increased non-linearly with graphene concentration. The corrosion resistance of the coatings with graphene was significantly improved. The wear resistance of the coatings was also greatly improved apart from the coating with 3 g/L graphene. The coating produced in the electrolyte with 2 g/L graphene exhibited the optimal comprehensive properties because graphene successfully incorporated into the coating via the pores and spread on the surface of the coating.
Optical and Chromaticity Properties of Metal-Dielectric Composite-Based Multilayer Thin-Film Structures Prepared by RF Magnetron Sputtering
Authors: Nur-E-Alam Mohammad , Rahman Md Momtazur , Basher Mohammad Khairul , Vasiliev Mikhail , Alameh Kamal
Published: March 2020

Coated glass products, and especially the low-emissivity coatings, have become a common building material used in modern architectural projects. More recently, these material systems became common in specialized glazing systems featuring solar energy harvesting. Apart from achieving the stability of optical parameters in multilayer coatings, it is also important to have improved control over the design of visual color properties of the coated glass. We prepare metal-dielectric composite (MDC)-based multilayer thin-film structures using the radio frequency (RF)-magnetron sputtering deposition and report on their optical and chromaticity properties in comparison with these obtained using pure metal-based Dielectric/Metal/Dielectric (DMD) trilayer structures of similar compositions. Experimentally achieved Hunter L, a, b values of MDC-based multilayer building blocks of coatings provide a new outlook on the engineering of future-generation optical coatings with better color consistency and developing approaches to broaden the range of achievable color coordinates and better environmental stability.
Characterization of High-Temperature Hierarchical Porous Mullite Washcoat Synthesized Using Aluminum Dross and Coal Fly Ash
Authors: Choo Thye Foo , Mohd Salleh Mohamad Amran , Kok Kuan Ying , Matori Khamirul Amin , Abdul Rashid Suraya
Published: March 2020

Mixture of aluminum dross (AD) and coal fly ash (CFA) was used to produce high-temperature porous mullite for washcoat application. CFA is the combustion by-product of pulverized coal in a coal-fired power plant, while AD is a waste product produced in secondary aluminum refining. In this study, 80 wt% of AD and 20 wt% of CFA was used to prepare a mullite precursor (MP) via acid leaching and dry-milling. The precursor was coated on a substrate and subsequently fired at 1500 °C. The results showed that the precursor transformed to a hierarchical porous microstructure assembled by large interlocked acicular mullite crystals. The pore structures consisted of large interconnected open pores and small pores. The specific surface area of the mullite washcoat was 4.85 m2g−1 after heating at 1500 °C for 4 h. The specific surface area was compatible with the specific surface area of other high-temperature washcoats.
Revisiting the Zintl‒Klemm Concept for ALn2Ag3Te5-Type Alkaline-Metal (A) Lanthanide (Ln) Silver Tellurides
Authors: Eickmeier Katharina , Fries Kai , Gladisch Fabian , Dronskowski Richard , Steinberg Simon
Published: March 2020

Understanding the bonding nature of solids is decisive, as knowledge of the bonding situation for any given material provides valuable information about its structural preferences and physical properties. Although solid-state tellurides are at the forefront of several fields of research, the electronic structures, particularly their nature of bonding, are typically understood by applying the Zintl‒Klemm concept. However, certain tellurides comprise ionic as well as strong (polar) mixed-metal bonds, in obvious contrast to the full valence-electron transfers expected by Zintl‒Klemm’s reasoning. How are the valence-electrons really distributed in tellurides containing ionic as well as mixed-metal bonds? To answer this question, we carried out bonding and Mulliken as well as Löwdin population analyses for the series of ALn2Ag3Te5-type tellurides (A = alkaline-metal; Ln = lanthanide). In addition to the bonding analyses, we provide a brief description of the crystal structure of this particular type of telluride, using the examples of RbLn2Ag3Te5 (Ln = Ho, Er) and CsLn2Ag3Te5 (Ln = La, Ce), which have been determined for the first time.
Proposal and Thermodynamic Assessment of S-CO2 Brayton Cycle Layout for Improved Heat Recovery
Authors: Siddiqui Muhammad Ehtisham , Almitani Khalid H.
Published: March 2020

This article deals with the thermodynamic assessment of supercritical carbon dioxide (S-CO2) Brayton power cycles. The main advantage of S-CO2 cycles is the capability of achieving higher efficiencies at significantly lower temperatures in comparison to conventional steam Rankine cycles. In the past decade, variety of configurations and layouts of S-CO2 cycles have been investigated targeting efficiency improvement. In this paper, four different layouts have been studied (with and without reheat): Simple Brayton cycle, Recompression Brayton cycle, Recompression Brayton cycle with partial cooling and the proposed layout called Recompression Brayton cycle with partial cooling and improved heat recovery (RBC-PC-IHR). Energetic and exergetic performances of all configurations were analyzed. Simple configuration is the least efficient due to poor heat recovery mechanism. RBC-PC-IHR layout achieved the best thermal performance in both reheat and no reheat configurations ( η t h   = 59.7% with reheat and η t h   = 58.2 without reheat at 850 °C), which was due to better heat recovery in comparison to other layouts. The detailed component-wise exergy analysis shows that the turbines and compressors have minimal contribution towards exergy destruction in comparison to what is lost by heat exchangers and heat source.
A Geometric Approach to Average Problems on Multinomial and Negative Multinomial Models
Authors: Li Mingming , Sun Huafei , Li Didong
Published: March 2020

This paper is concerned with the formulation and computation of average problems on the multinomial and negative multinomial models. It can be deduced that the multinomial and negative multinomial models admit complementary geometric structures. Firstly, we investigate these geometric structures by providing various useful pre-derived expressions of some fundamental geometric quantities, such as Fisher-Riemannian metrics, α -connections and α -curvatures. Then, we proceed to consider some average methods based on these geometric structures. Specifically, we study the formulation and computation of the midpoint of two points and the Karcher mean of multiple points. In conclusion, we find some parallel results for the average problems on these two complementary models.
Comparative Evaluation of Lifetime of Three-Level Inverters in Grid-Connected Photovoltaic Systems
Authors: Choi Ui-Min , Lee June-Seok
Published: March 2020

The cost of the PV energy reduction is still required to increase the penetration level of PV systems in the energy market. The reliability of PV inverters is one of the important aspects to be enhanced in order to reduce the cost of PV energy, since it is closely related to the maintenance cost and the annual energy production. In this paper, the lifetime of NPC and T-type inverters, which are three-level inverter topologies that are widely used for PV systems, are comparatively evaluated with a 30 kW grid-connected PV system. It is performed by focusing on power devices since the power electronic components of both converters are the same except for the power devices. Therefore, this result can represent the comparison of the reliability performance of the NPC and T-type inverters. The power loss and temperature distributions of power devices are analyzed and their efficiencies are compared at different power levels with different switching frequencies. The lifetimes of the reliability-critical power devices in the NPC and T-type inverters are estimated, respectively with a one-year mission profile of the PV system, and the results are compared.
Hydrothermal Depolymerization of Biorefinery Lignin-Rich Streams: Influence of Reaction Conditions and Catalytic Additives on the Organic Monomers Yields in Biocrude and Aqueous Phase
Authors: Dell’Orco Stefano , Miliotti Edoardo , Lotti Giulia , Rizzo Andrea Maria , Rosi Luca , Chiaramonti David
Published: March 2020

Hydrothermal depolymerization of lignin-rich streams (LRS) from lignocellulosic ethanol was successfully carried out in a lab-scale batch reactors unit. A partial depolymerization into oligomers and monomers was achieved using subcritical water as reaction medium. The influence of temperature (300–350–370 °C) and time (5–10 minutes) was investigated to identify the optimal condition on the monomers yields in the lighter biocrude (BC1) and aqueous phase (AP) fractions, focusing on specific phenolic classes as well as carboxylic acids and alcohols. The effect of base catalyzed reactions (2–4 wt. % of KOH) was compared to the control tests as well as to acid-catalyzed reactions obtained with a biphasic medium of supercritical carbon dioxide (sCO2) and subcritical water. KOH addition resulted in enhanced overall depolymerization without showing a strong influence on the phenolic generation, whereas sCO2 demonstrated higher phenolic selectivity even though no effect was observed on the overall products mass yields. In conclusion, a comparison between two different biocrude collection procedures was carried out in order to understand how the selected chemical extraction mode influences the distribution of compounds between BC1 and AP.
Energy Gain in Passive Solar Greenhouses Due to CO2 Enrichment
Authors: Martzopoulou Anastasia , Vafiadis Dimitris , Fragos Vassilios
Published: March 2020

The production cost of greenhouse cultivation depends mainly upon significant amounts of energy consumption in order to keep the optimum environmental condition for plant growth. Τhe expenditure on energy, either for heating or cooling, ranges between 30% to 60% of the total production costs, and any attempt to save energy will result in a positive effect on the potentiality of production accordingly, affecting the greenhouse product prices. Research has shown that CO2 enrichment in greenhouses significantly increases the yield of most indoor cultivation of plants of the C3 category. For these plants, when the CO2 concentration increases by three times above that of the atmosphere (380 ppm), the optimum plant growth temperature shifts higher by 5 °C to 10 °C reaching up to 30 °C to 32 °C. Therefore, huge amounts of solar energy can be captured inside the greenhouses, as the ventilation can be decreased. Alongside this, the use of a simple passive solar system consisting of plastic sleeves filled with water is considered to be an improved way to increase the energy inside greenhouses. In this work, three experimental trials were conducted to examine the benefit of the solar energy captured inside a greenhouse during CO2 enrichment at high temperatures. Finally, a modeling approach based on the heat loss equation was developed in order to establish the energy saving inside the greenhouses under the circumstances mentioned.
The Cost-Optimal Analysis of a Multistory Building in the Mediterranean Area: Financial and Macroeconomic Projections
Authors: Bruno Roberto , Bevilacqua Piero , Carpino Cristina , Arcuri Natale
Published: March 2020

Cost-optimal analysis was pointed out in the 2010/31 European Directive as a tool to evaluate the achievable building energy performance levels as a function of the corresponding costs. These analyses can be carried out by a financial projection for private investors and a macroeconomic approach to establish the minimal energy performance levels. Consequently, the financial projection provides different results that could stimulate private investors toward other cost-optimal solutions that do not match the minimal energy performance levels. For this purpose, both the projections were analyzed in the BEopt environment, developed by NREL, on a multistory building located in two contrasting climatic zones of the Mediterranean area, one cold and the other warm, highlighting the differences. The cost-optimal solutions were identified by a parametric study involving measures that affect thermal losses and solar gains, whereas the air-conditioning plant was left unchanged in order to include a fraction of renewable energy in the coverage of the building demands. Results showed that both the projections produced the same cost-optimal solutions, however, the latter matches the building designed to fulfill the minimal energy performance levels only in the cold climate. Conversely, noticeable deviations were detected in the warm location, therefore minimal energy performance levels should be revised, with preference for less insulated opaque surfaces and better performing glazing systems. Moreover, the macroeconomic scenario returns a more limited distance between the minimal energy performance levels and the cost-optimal solutions, therefore, it is far from the real economic frame sustained by private investors.
Wave-Turbulence Decomposition Methods Applied to Tidal Energy Site Assessment
Authors: Perez Larissa , Cossu Remo , Couzi Camille , Penesis Irene
Published: March 2020

High levels of turbulence have been proven to substantially increase the blade loadings on tidal turbines, outlining the need of properly characterizing turbulence parameters in tidal energy sites. The presence of long surface gravity waves may cause a significant bias on the estimation of these parameters, which requires wave-turbulence decomposition methods that are currently missing from guidelines. Here, three techniques of decomposing wave and turbulence are tested: the stopband filter (SB), moving average filter (MA), and synchrosqueezing wavelet transform (SWT). The study site, Banks Strait, Tasmania, is a 16 km wide channel that presents high potential for tidal energy generation. Wave peak periods at the study site were found to vary mostly between 7 and 12 s, with maximum exceeding 15 s. Turbulence intensities (TI), turbulent kinetic energy (TKE), and integral scales are quantified. Our results indicate differences between the estimates obtained from each method. The MA highly underestimates turbulence, resulting in TI values which were nearly 50% lower than those obtained from other decomposition methods. While TI and TKE estimated from the SB and the SWT techniques are quite similar, integral length scales are considerably underestimated by the SB. These findings reveal that the SWT is a more reliable method because of the more accurate estimates of turbulence parameters and indicate the need of establishing guidelines which address wave-turbulence decomposition in tidal stream energy site assessments. Despite having shown to be quite a versatile technique, further investigation of its applicability in data from other prospective tidal energy sites is necessary to fully assess the generality of the SWT technique.
Multi-Sensor Prediction of Stand Volume by a Hybrid Model of Support Vector Machine for Regression Kriging
Authors: Chen Lin , Ren Chunying , Zhang Bai , Wang Zongming
Published: March 2020

Quantifying stand volume through open-access satellite remote sensing data supports proper management of forest stand. Because of limitations on single sensor and support vector machine for regression (SVR) as well as benefits from hybrid models, this study innovatively builds a hybrid model as support vector machine for regression kriging (SVRK) to map stand volume of the Changbai Mountains mixed forests covering 171,450 ha area based on a small training dataset (n = 928). This SVRK model integrated SVR and its residuals interpolated by ordinary kriging. To determine the importance of multi-sensor predictors from ALOS and Sentinel series, the increase in root mean square error (RMSE) of SVR was calculated by removing the variable after the standardization. The SVRK model achieved accuracy with mean error, RMSE and correlation coefficient in –2.67%, 25.30% and 0.76, respectively, based on an independent dataset (n = 464). The SVRK improved the accuracy of 9% than SVR based on RMSE values. Topographic indices from L band InSAR, backscatters of L band SAR, and texture features of VV channel from C band SAR, as well as vegetation indices of the optical sensor were contributive to explain spatial variations of stand volume. This study concluded that SVRK was a promising approach for mapping stand volume in the heterogeneous temperate forests with limited samples.
Disturbance Effects on Soil Carbon and Greenhouse Gas Emissions in Forest Ecosystems
Authors: Cai Yanjiang , Chang Scott X
Published: March 2020

Forests cover around 30% of the global land area and forest ecosystems can store over 70% of total soil organic carbon (SOC) of all terrestrial ecosystems, but SOC stocks and greenhouse gas (GHG) emissions may be affected by both natural and anthropogenic disturbances. Even though the changes in forest soil C pool can have a significant effect on climate change, there are some contradictory results regarding the role of forest disturbance on SOC sequestration, GHG emissions, and the mitigation of global changes. Therefore, there is a need to better understand the impact of different disturbance regimes on forest soil C storage and GHG emissions. A Special Issue was therefore organized for discussing the responses of soil C storage and GHG emissions to various types of disturbances in forest ecosystems and a total of 15 studies were accepted for this special issue to assess these responses. This Special Issue includes the effects of storms and beetle outbreaks, Karstification, rock desertification, warming, nitrogen addition, land-use change, field tillage, and biochar application on soil C dynamics and/or GHG emissions.
Response of Different Treatment Protocols to Treat Chronic Non-Bacterial Osteomyelitis (CNO) of the Mandible in Adult Patients: A Systematic Review
Authors: Timme Maximilian , Bohner Lauren , Huss Sebastian , Kleinheinz Johannes , Hanisch Marcel
Published: March 2020

(1) Background: Chronic non-bacterial osteomyelitis (CNO) is an autoinflammatory bone disease of finally unknown etiology, which can occur alone or related with syndromes (chronic recurrent multifocal osteomyelitis—CRMO; synovitis, acne, pustulosis, hyperostosis and osteitis syndrome—SAPHO). The involvement of the mandible is rather rare. (2) Methods: We carried out a systematic literature search on CNO with mandibular involvement, according to the “Preferred Reporting Items for Systematic Reviews and Meta-Analyses” (PRISMA) guidelines, considering the different synonyms for CNO, with a special focus on therapy. (3) Results: Finally, only four studies could be included. A total of 36 patients were treated in these studies—therefore, at most, only tendencies could be identified. The therapy in the included works was inconsistent. Various therapies could alleviate the symptoms of the disease. A complete remission could only rarely be observed and is also to be viewed against the background of the fluctuating character of the disease. The success of one-off interventions is unlikely overall, and the need for long-term therapies seems to be indicated. Non-steroidal anti-inflammatory drugs (NSAIDs) were not part of any effective therapy. Surgical therapy should not be the first choice. (4) Conclusions: In summary, no evidence-based therapy recommendation can be given today. For the future, systematic clinical trials on therapy for CNO are desirable.
The Associations between Family-Related Factors and Excessive Internet Use in Adolescents
Authors: Faltýnková Anna , Blinka Lukas , Ševčíková Anna , Husarova Daniela
Published: March 2020

This study examined the relationship between Excessive Internet Use (EIU) in adolescents and their family environment, namely the family type, the family economic status, the effect of parental care, the level of parental control, the amount of parental monitoring, the quality of communication, and the time spent together. The study was based on data from an international survey, Health Behaviour in School Aged Children (HBSC), conducted in Slovakia. The sample representative for adolescents included 2547 participants (51% boys) aged 13–15. Multiple-step linear regression revealed that higher parental care and parental monitoring predicted lower EIU, while higher parental overprotection and lower socioeconomic status predicted higher EIU. The results suggest that both so-called optimal parenting (i.e., the balance of emotional warmth and protection) and the adolescent′s autonomy lower the risk of EIU. Family factors explained about 14% of the variance, which suggests that aside from personal, cognitive and affective factors, a close social environment also plays an important role in adolescence EIU.
Associations of Weight-Adjusted Body Fat and Fat Distribution with Bone Mineral Density in Chinese Children Aged 6–10 Years
Authors: Liang Jingjing , Chen Yongxin , Zhang Jiahua , Ma Bingjie , Hu Yan , Liu Yi , Lin Suifang , Zhang Zheqing , Song Yanyan
Published: March 2020

Although obesity is considered osteoprotective, the effects of body fat and fat distribution on bone tissue after adjusting for the effects of body weight remain uncertain. This study evaluated the relationships between fat mass, fat distribution, and bone mineral status beyond its weight-bearing effect. We recruited 466 children aged 6–10 years in China. Dual-energy X-ray absorptiometry was used to determine the bone mineral density (BMD) and bone mineral content (BMC) in the total body and total body less head (TBLH), as well as the fat mass (FM) and percentage fat mass (%FM) of the total and segmental body. Weight-adjusted measures of FM and %FM were derived using the residual method. After adjusting for the effects of covariates, we observed statistically significant, dose-dependent negative relationships between the TBLH·BMD/BMC and various weight-adjusted measures of body fat (p for trend: <0.001–0.038). For each standard deviation increment in the weight-adjusted total body, TBLH, trunk and limbs, the size-adjusted BMC decreased approximately 9.44, 9.28, 8.13, and 6.65 g in boys, respectively, and by approximately 13.74, 13.71, 7.84, and 12.95 g in girls, respectively. Significant inverse associations between FM accumulation in the total body and most body parts with the BMD/BMC were observed in both boys and girls after adjusting for weight and potential confounders.
Chronic Exposure to Low Concentration Lead Chloride-Induced Anxiety and Loss of Aggression and Memory in Zebrafish
Authors: Bui Thi Ngoc Hieu , Nguyen Thi Ngoc Anh , Audira Gilbert , Siregar Petrus , Liang Sung-Tzu , Huang Jong-Chin , Hsiao Chung-Der
Published: March 2020

Lead and lead-derived compounds have been extensively utilized in industry, and their chronic toxicity towards aquatic animals has not been thoroughly addressed at a behavioral level. In this study, we assessed the risk of exposure to lead at a waterborne environmental concentration in adult zebrafish by behavioral and biochemical analyses. Nine tests, including three-dimension (3D) locomotion, novel tank exploration, mirror biting, predator avoidance, social interaction, shoaling, circadian rhythm locomotor activity, color preference, and a short-term memory test, were performed to assess the behavior of adult zebrafish after the exposure to 50 ppb PbCl2 for one month. The brain tissues were dissected and subjected to biochemical assays to measure the relative expression of stress biomarkers and neurotransmitters to elucidate the underlying mechanisms for behavioral alterations. The results of the behavioral tests showed that chronic exposure to lead could elevate the stress and anxiety levels characterized by elevated freezing and reduced exploratory behaviors. The chronic exposure to PbCl2 at a low concentration also induced a sharp reduction of aggressiveness and short-term memory. However, no significant change was found in predator avoidance, social interaction, shoaling, or color preference. The biochemical assays showed elevated cortisol and reduced serotonin and melatonin levels in the brain, thus, altering the behavior of the PbCl2-exposed zebrafish. In general, this study determined the potential ecotoxicity of long-term lead exposure in adult zebrafish through multiple behavioral assessments. The significant findings were that even at a low concentration, long-term exposure to lead could impair the memory and cause a decrease in the aggressiveness and exploratory activities of zebrafish, which may reduce their survival fitness.
Antibiotic Resistance, Virulence Factors, Phenotyping, and Genotyping of Non-Escherichia coli Enterobacterales from the Gut Microbiota of Healthy Subjects
Authors: Amaretti Alberto , Righini Lucia , Candeliere Francesco , Musmeci Eliana , Bonvicini Francesca , Gentilomi Giovanna Angela , Rossi Maddalena , Raimondi Stefano
Published: March 2020

Non-Escherichia coli Enterobacterales (NECE) can colonize the human gut and may present virulence determinants and phenotypes that represent severe heath concerns. Most information is available for virulent NECE strains, isolated from patients with an ongoing infection, while the commensal NECE population of healthy subjects is understudied. In this study, 32 NECE strains were isolated from the feces of 20 healthy adults. 16S rRNA gene sequencing and mass spectrometry attributed the isolates to Klebsiella pneumoniae, Klebsiella oxytoca, Enterobacter cloacae, Enterobacter aerogenes, Enterobacter kobei, Citrobacter freundii, Citrobacter amalonaticus, Cronobacter sp., and Hafnia alvei, Morganella morganii, and Serratia liquefaciens. Multiplex PCR revealed that K. pneumoniae harbored virulence genes for adhesins (mrkD, ycfM, and kpn) and enterobactin (entB) and, in one case, also for yersiniabactin (ybtS, irp1, irp2, and fyuA). Virulence genes were less numerous in the other NECE species. Biofilm formation was spread across all the species, while curli and cellulose were mainly produced by Citrobacter and Enterobacter. Among the most common antibiotics, amoxicillin-clavulanic acid was the sole against which resistance was observed, only Klebsiella strains being susceptible. The NECE inhabiting the intestine of healthy subjects have traits that may pose a health threat, taking into account the possibility of horizontal gene transfer.
Radiosensitization of Hepatocellular Carcinoma through Targeting Radio-Associated MicroRNA
Authors: Wu Cheng-Heng , Chen Cheng-Yi , Yeh Chau-Ting , Lin Kwang-Huei
Published: March 2020

Hepatocellular carcinoma (HCC) is the fourth leading cause of cancer-related deaths worldwide. For patients who are resistant to monotherapy, multimodal therapy is a basic oncologic principle that incorporates surgery, radiotherapy (RT), and chemotherapy providing survival benefits for patients with most types of cancer. Although liver has low tolerance for radiation, high-precision RT for local HCC minimizes the likelihood of radiation-induced liver disease (RILD) in noncancerous liver tissue. RT have several therapeutic benefits, including the down-staging of tumors to make them resectable and repression of metastasis. The DNA damage response (DDR) is a cellular response to irradiation (IR), including DNA repair of injured cells and induction of programmed cell death, thereby resulting in maintenance of cell homeostasis. Molecules that block the activity of proteins in DDR pathways have been found to enhance radiotherapeutic effects. These molecules include antibodies, kinase inhibitors, siRNAs and miRNAs. MicroRNAs (miRNAs) are short non-coding regulatory RNAs binding to the 3′-untranslated regions (3′-UTR) of the messenger RNAs (mRNAs) of target genes, regulating their translation and expression of proteins. Thus, miRNAs and their target genes constitute complicated interactive networks, which interact with other molecules during carcinogenesis. Due to their promising roles in carcinogenesis, miRNAs were shown to be the potential factors that mediated radiosensitivity and optimized outcomes of the combination of systemic therapy and radiotherapy.
Intermittent Energy Restriction Attenuates the Loss of Fat Free Mass in Resistance Trained Individuals. A Randomized Controlled Trial
Authors: Campbell Bill I. , Aguilar Danielle , Colenso-Semple Lauren M. , Hartke Kevin , Fleming Abby R. , Fox Carl D. , Longstrom Jaymes M. , Rogers Gavin E. , Mathas David B. , Wong Vickie , Ford Sarah , Gorman John
Published: March 2020

There is a lack of research into how lean, resistance trained (RT) individuals respond to intermittent energy restricted diets. Therefore, we investigated body composition changes in RT-individuals during continuous energy restriction or intermittent restriction. A total of 27 males and females (25 ± 6.1 years; 169 ± 9.4 cm; 80 ± 15.6 kg) were randomized to a ~25% caloric restricted diet Refeed (RF; n = 13) or Continuous group (CN; n = 14) in conjunction with 4-days/week resistance training for 7-weeks. RF implemented two consecutive days of elevated carbohydrate (CHO) intake, followed by 5-days of caloric restriction each week. CN adhered to a continuous 7-week caloric restriction. Body mass (BM), fat mass (FM), fat-free mass (FFM), dry fat-free mass (dFFM), and resting metabolic rate (RMR) were assessed pre/post-diet. Both groups significantly reduced BM (RF: baseline = 76.4 ± 15.6 kg, post-diet = 73.2 ± 13.8 kg, Δ3.2 kg; CN: baseline = 83.1 ± 15.4 kg, post-diet = 79.5 ± 15 kg, Δ3.6 kg) and FM (RF: baseline = 16.3 ± 4 kg, post-diet = 13.5 ± 3.6 kg, Δ2.8 kg; CN: baseline = 16.7 ± 4.5 kg, post-diet = 14.4 ± 4.9 kg, Δ2.3 kg) with no differences between groups. FFM (RF: baseline = 60.1 ± 13.8 kg, post-diet = 59.7 ± 13.0 kg, 0.4 kg; CN: baseline = 66.4 ± 15.2 kg, post-diet = 65.1 ± 15.2 kg, Δ1.3 kg p = 0.006), dFFM (RF: baseline = 18.7 ± 5.0 kg, post-diet = 18.5 ± 4.5 kg, Δ0.2 kg; CN: baseline =21.9 ± 5.7 kg, post-diet = 20.0 ± 5.7 kg, Δ1.9 kg), and RMR (RF: baseline = 1703 ± 294, post-diet = 1665 ± 270, Δ38 kcals; CN: baseline = 1867 ± 342, post-diet = 1789 ± 409, Δ78 kcals) were better maintained in the RF group. A 2-day carbohydrate refeed preserves FFM, dryFFM, and RMR during energy restriction compared to continuous energy restriction in RT-individuals.
Design and Optimization Lattice Endoprosthesis for Long Bones: Manufacturing and Clinical Experiment
Authors: Bolshakov Pavel , Raginov Ivan , Egorov Vladislav , Kashapova Regina , Kashapov Ramil , Baltina Tatyana , Sachenkov Oskar
Published: March 2020

The article is devoted to the construction of lattice endoprosthesis for a long bone. Clinically, the main idea is to design a construction with the ability to improve bone growth. The article presents the algorithm for such a design. The construction should be produced by additive manufacturing. Such an approach allows using not only metallic materials but also ceramics and polymers. The algorithm is based on the influence function as a method to describe the elementary cell geometry. The elementary cell can be described by a number of parameters. The influence function maps the parameters to local stress in construction. Changing the parameters influences the stress distribution in the endoprosthesis. In the paper, a bipyramid was used as an elementary cell. Numerical studies were performed using the finite element method. As a result, manufacturing construction is described. Some problems for different orientations of growth are given. The clinical test was done and histological results were presented.
Reactive Insertion of PEDOT-PSS in SWCNT@Silica Composites and its Electrochemical Performance
Authors: Djelad Halima , Benyoucef Abdelghani , Morallón Emilia , Montilla Francisco
Published: March 2020

Hybrid silica-modified materials were synthesized on glassy carbon (GC) electrodes by electroassisted deposition of sol-gel precursors. Single-wall carbon nanotubes (SWCNTs) were dispersed in a silica matrix (SWCNT@SiO2) to enhance the electrochemical performance of an inorganic matrix. The electrochemical behavior of the composite electrodes was tested against the ferrocene redox probe. The SWCNT@SiO2 presents an improvement in the electrochemical performance towards ferrocene. The heterogeneous rate constant of the SWCNT@SiO2 can be enhanced by the insertion of poly(3,4-Ethylendioxythiophene)-poly(sodium 4-styrenesulfonate) PEDOT-PSS within the silica matrix, and this composite was synthesized successfully by reactive electrochemical polymerization of the precursor EDOT in aqueous solution. The SWCNT@SiO2-PEDOT-PSS composite electrodes showed a heterogeneous rate constant more than three times higher than the electrode without conducting polymer. Similarly, the electroactive area was also enhanced to more than twice the area of SWCNT@SiO2-modified electrodes. The morphology of the sample films was analyzed by scanning electron microscopy (SEM).
The Influence of Plasma-Assisted Production and Milling Processes of DLC Flakes on Their Size, Composition and Chemical Structure
Authors: Kaźmierczak Tomasz , Niedzielski Piotr , Kaczorowski Witold
Published: March 2020

Diamond-like carbon (DLC) flakes were produced using a dual-frequency method: microwave/radiofrequency plasma-assisted chemical vapour deposition (MW/RF PACVD) with the use of methane or its mixture with gases such as hydrogen, argon, oxygen or nitrogen. Their modification was performed using a planetary ball mill with and without a fluid: deionised water or methanol. Changes occurring in the morphology of flake surfaces were presented in pictures taken using a scanning electron microscope (SEM). Their composition and chemical structure were analysed using Raman spectroscopy and X-ray photoelectron spectroscopy (XPS). The presented research results show that it is possible to control the size of flakes and their chemical structure. An increase in the C-C sp3 bond content in produced carbon-based materials is only possible by modifying DLC flakes during their production process by introducing oxygen or argon into the working chamber together with the carbon-carrying gas. In the processes of mechanical DLC flake modification, it is necessary to add fluid to limit the occurrence of graphitisation processes. The research conducted shows that methanol is best used for this purpose as its use results in a decrease in the percentage of C-C sp3 bonds as compared to the materials, before milling, of only 1.7%. A frequent problem both in the production of DLC flakes and during their mechanical modification is the introduction of additional elements into their structure. Admixing electrode materials from the plasma-chemical device (iron) or grinding beads (zirconium) to DLC flakes was observed in our studies. These processes can be limited by the appropriate selection of production conditions or by mechanical modifications.
Mechanical and Electrical Characteristics of WB2 Synthesized at High Pressure and High Temperature
Authors: Wang Changchun , Song Lele , Xie Yupeng
Published: March 2020

Single-phase tungsten diboride (WB2) was synthesized at high pressure and high temperature. The different grain sizes ranging from 300 nm to 3 µm were successfully obtained in WB2 by controlling the experimental conditions. The effects of grain size on hardness and resistivity properties were investigated. The Vickers hardness of WB2 was modulated with grain size. The maximum asymptotic Vickers hardness is 25.5 GPa for WB2 with a grain size of 300 nm which is a 10% increase compared to WB2 with a grain size of 3 µm. The optimal electrical resistivity of WB2 was 10−7 Ωm with the biggest grain size of 3 µm, which is ascribed to low grain boundary density. The superior properties of hardness and electrical resistivity demonstrate that WB2 should be a new functional hard material replacing WC which is widely used in industrial production.
Highly Sensitive THz Gas-Sensor Based on the Guided Bloch Surface Wave Resonance in Polymeric Photonic Crystals
Authors: Zhang Chi , Shen Suling , Wang Qiong , Lin Mi , Ouyang Zhengbiao , Liu Qiang
Published: March 2020

THz waves have interesting applications in refractive index sensing. A THz gas sensor based on the guided Bloch surface wave resonance (GBSWR) in a one-dimensional photonic crystal (1DPhC), which consists of periodic polycarbonate (PC) layers and polyvinylidene fluoride (PVDF) layers, has been proposed. Numerical results based on finite element method (FEM) show that the photonic band gap that confines Bloch surface waves (BSWs) lies in the regime of 11.54 to 21.43 THz, in which THz wave can transmit in both PC and PVDF with the ignored absorption. The calculated sensitivity of hazardous gas HCN in angle is found to be 118.6°/RIU (and the corresponding figure of merit (FOM) is 227) and the sensitivity in frequency is 4.7 THz/RIU (the corresponding FOM is 301.3). The proposed structure may also be used for monitoring hazardous gases which show absorption to the incident THz wave. Further results show that for N2O gas, the maximum sensitivity goes up to 644 (transmittance unit/ one unit of the imaginary part of the refractive index). The proposed design may find applications in the detection of dangerous gases.
Novel Approach of Nanostructured Bainitic Steels’ Production with Improved Toughness and Strength
Authors: Kirbiš Peter , Anžel Ivan , Rudolf Rebeka , Brunčko Mihael
Published: March 2020

The tendencies of development within the field of engineering materials show a persistent trend towards the increase of strength and toughness. This pressure is particularly pronounced in the field of steels, since they compete with light alloys and composite materials in many applications. The improvement of steels’ mechanical properties is sought to be achieved with the formation of exceptionally fine microstructures ranging well into the nanoscale, which enable a substantial increase in strength without being detrimental to toughness. The preferred route by which such a structure can be produced is not by applying the external plastic deformation, but by controlling the phase transformation from austenite into ferrite at low temperatures. The formation of bainite in steels at temperatures lower than about 200 °C enables the obtainment of the bulk nanostructured materials purely by heat treatment. This offers the advantages of high productivity, as well as few constraints in regard to the shape and size of the workpiece when compared with other methods for the production of nanostructured metals. The development of novel bainitic steels was based on high Si or high Al alloys. These groups of steels distinguish a very fine microstructure, comprised predominantly of bainitic ferrite plates, and a small fraction of retained austenite, as well as carbides. The very fine structure, within which the thickness of individual bainitic ferrite plates can be as thin as 5 nm, is obtained purely by quenching and natural ageing, without the use of isothermal transformation, which is characteristic for most bainitic steels. By virtue of their fine structure and low retained austenite content, this group of steels can develop a very high hardness of up to 65 HRC, while retaining a considerable level of impact toughness. The mechanical properties were evaluated by hardness measurements, impact testing of notched and unnotched specimens, as well as compression and tensile tests. Additionally, the steels’ microstructures were characterised using light microscopy, field emission scanning electron microscopy (FESEM) and high-resolution transmission electron microscopy (HRTEM). The obtained results confirmed that the strong refinement of the microstructural elements in the steels results in a combination of extremely high strength and very good toughness.
On Row Sequences of Hermite–Padé Approximation and Its Generalizations
Authors: Bosuwan Nattapong
Published: March 2020

Hermite–Padé approximation has been a mainstay of approximation theory since the concept was introduced by Charles Hermite in his proof of the transcendence of e in 1873. This subject occupies a large place in the literature and it has applications in different subjects. Most of the studies of Hermite–Padé approximation have mainly concentrated on diagonal sequences. Recently, there were some significant contributions in the direction of row sequences of Type II Hermite–Padé approximation. Moreover, various generalizations of Type II Hermite–Padé approximation were introduced and studied on row sequences. The purpose of this paper is to reflect the current state of the study of Type II Hermite–Padé approximation and its generalizations on row sequences. In particular, we focus on the relationship between the convergence of zeros of the common denominators of such approximants and singularities of the vector of approximated functions. Some conjectures concerning these studies are posed.