bluuebunny/crossref_metadata_2025 huggingface dataset:
The Regeneration of Urban Blue Spaces: A Public Health Intervention? Reviewing the Evidence
Authors: Brückner Anna , Falkenberg Timo , Heinzel Christine , Kistemann Thomas
Published: January 2022

Research in recent years has demonstrated that urban surface waters (“urban blue spaces”) can provide beneficial effects on human health and wellbeing. Despite blue spaces prevailing on urban development agendas across the world, little investigation has been done whether and how the regeneration of such spaces is used as a (community-based) public health intervention. Therefore, a review was conducted to analyze urban blue space regeneration projects in terms of their significance for public health. Results show that the regeneration of urban blue spaces displays a diversity of intervention types and follows certain development trends seen in general urban regeneration: Similarities mainly arise in relation to objectives (multi-dimensional goals with increasing focus on environmental sustainability and economic interests), stakeholders (shift to multi-actor governance with a rise of partnerships and community participation), and funding (prevalence of mixed financial schemes and increasing reliance on external funding sources). Although threefold public health effects have been noted across the projects (i. behavioral changes toward healthier lifestyles, ii. healthier urban environments, iii. health policy changes), results of this review indicate that the potential to use urban blue regeneration as a community-based health intervention has yet to be realized.
Requirements for Creating a Position for Community Health Nursing Within the Iranian Primary Health Care System: A SWOT Analysis
Authors: Hosseinnejad Aazam , Rassouli Maryam , Jahani Simin , Elahi Nasrin , Molavynejad Shahram
Published: January 2022

Background: Accepting community health nursing in the primary care system of each country and focusing on creating a position for community health nurses is of significant importance. The aim of this study was to examine the stakeholders' perception of the requirements for establishing a position for community health nursing in the Iranian primary health care system.

Methods: This qualitative study was done using 24 semi-structured interviews conducted from May 2020 to February 2021 in Iran. The participants were selected through purposive sampling and consisted of nursing policy makers, the policy makers of the Health Deputy of Ministry of Health, the managers and the authorities of universities of medical sciences all across the country, community health nursing faculty members, and community health nurses working in health care centers. After recording and transcribing the data, data analysis was performed in MAXQDA10 software, using Elo and Kyngas's directed content analysis approach and based on WHO's community health nursing role enhancement model. The statements for each main category were summarized in SWOT classification. To examine the trustworthiness of the data, Lincoln & Guba's criteria were used.

Results: By analyzing the interviews 6 main categories identified consist of creating a transparent framework for community health nursing practice, enhancing community health nursing education and training for practice in the primary health care system and community settings, seeking support, strengthening the cooperation and engagement among the key stakeholders of the primary health care system, changing the policies and the structure of the health system, and focusing on the deficiencies of the health system. Each main categories including the subcategories strengths, weaknesses, opportunities and threats (SWOT).

Conclusions: Based on the participants' opinions, focusing on the aforementioned dimensions is one of the requirements of developing a position for community health nursing within the Iranian PHC system. It seems that correct and proper implementation of these strategies in regard with the cultural context of society can help policymakers manage challenges that prevent the performance of community health nursing in the health system.
Challenges and Opportunities in Robotic Food Handling: A Review
Authors: Wang Zhongkui , Hirai Shinichi , Kawamura Sadao
Published: January 2022

Despite developments in robotics and automation technologies, several challenges need to be addressed to fulfill the high demand for automating various manufacturing processes in the food industry. In our opinion, these challenges can be classified as: the development of robotic end-effectors to cope with large variations of food products with high practicality and low cost, recognition of food products and materials in 3D scenario, better understanding of fundamental information of food products including food categorization and physical properties from the viewpoint of robotic handling. In this review, we first introduce the challenges in robotic food handling and then highlight the advances in robotic end-effectors, food recognition, and fundamental information of food products related to robotic food handling. Finally, future research directions and opportunities are discussed based on an analysis of the challenges and state-of-the-art developments.
Is There Any Non-functional Training? A Conceptual Review
Authors: Ide Bernardo N. , Silvatti Amanda P. , Marocolo Moacir , Santos Clarcson P. C. , Silva Bruno V. C. , Oranchuk Dustin J. , Mota Gustavo R.
Published: January 2022

This conceptual review investigates whetherfunctional training(FT) is a different approach from traditional strength, power, flexibility, and endurance (aerobic or cardiorespiratory) training already adopted in the physical training plan of professional, recreational athletes, healthy, and older adults. The 20 most recent papers published involving FT were searched in the PubMed/Medline database. Definition, concepts, benefits, and the exercises employed in FT programs were analyzed. The main results were: (a) there is no agreement about a universal definition for FT; (b) FT programs aim at developing the same benefits already induced by traditional training programs; (c) exercises employed are also the same. The inability to define FT makes the differentiation from traditional training programs difficult. Physical training programs can be easily described and classified as strength, power, flexibility, endurance, and the specific exercises employed (e.g., traditional resistance training, ballistic exercises, plyometrics and Olympic-style weightlifting, continuous and high-intensity interval training). This apt description and classification may provide consistent and clear communication between students, coaches, athletes, and sports scientists. Based on the current evidence and to avoid confusion and misconceptions, we recommend that the terms FT, high-intensity FT, and functional fitness training no longer describe any physical training program.
Electrohydrodynamic Drying in Agribusiness: Literature Review
Authors: Ciprian Foronda Kevin Daniel , Gafaro Garcés Delcy Camila , Restrepo Rendón Laura , Mendoza Alvites Yeyner Yamphier , Ricardo Sagra Joana Paola , Orozco Mendoza Gina Lia , Zartha Sossa Jhon Wilder
Published: January 2022

In agribusiness, drying is a unitary operation that optimizes the production and preservation of products and raw materials. Drying is performed through different traditional methods, one of the most recently studied is the electrohydrodynamic drying EHD which uses an electric field that allows decreasing the processing time thus increasing the drying speed of raw materials and consuming less energy. In this article, a review was carried out through Scopus using a search equation with the keywords “Electrohydrodynamic drying,” “food” and “AGRI” which resulted in a total of 145 articles; which were analyzed through in-depth reading, analyzing aspects such as year, author, keywords, countries, quartile, journal, relationship with agroindustry, mathematical models used and applications in agro-industrial products, this analysis was complemented with the application of Vantage Point software through co-occurrence matrices and cluster analysis. Recent applications were found in Carrot, Chicken, Sea Cucumber, Goji Berry, Peppermint Leaf, Quince, Potato, Blueberry, Aquatic Products, Banana Slices, Grape Pomace, Blueberry, Apple, Mushroom, Wheat, and Mushroom Slices, mathematical models with application in EHD drying were also found, such as Henderson and Pabis, Page, Logarithmic, Quadratic, Newton/Lewis, Diffusion and exponential.
Clinical Significance and Risk Factors of Local Recurrence in Synovial Sarcoma: A Retrospective Analysis of 171 Cases
Authors: Zhang Hongqiang , Huang Wending , Feng Qi , Sun Wei , Yan Wangjun , Wang Chunmeng , Zhang Jianing , Huang Kai , Yu Lin , Qu Xinglong , Chen Yong
Published: January 2022

Objective: To investigate risk factors of local recurrence of synovial sarcoma and the impact of local recurrence on survival.

Methods: We retrospectively reviewed clinical data of patients with II to IIIB (AJCC8) synovial sarcoma who underwent surgery at our center between March 2005 and December 2016. Data relating clinicopathological factors, treatment and prognosis were collected. The impact of local recurrence on overall survival (OS), local recurrence-free survival (LRFS), and distant relapse-free survival (DRFS) were analyzed. The prognostic factors associated with local recurrence were also analyzed using Kaplan-Meier Curves and Cox regression analysis.

Results: A total of 171 patients were included in this analysis. After a median follow-up of 48 months, 66 patients (38.6%) experienced local recurrence. The 5-year OS, LRFS, and DRFS rates of patients with local recurrence were 37.6, 6.1, and 24.1%, respectively. Multivariate analysis showed that larger initial tumors, multiple recurrences, positive resection margins, marginal resection, and lack of adjuvant therapy were associated with higher local recurrence.

Conclusion: Local recurrence of synovial sarcoma is associated with distant metastasis and poor survival. Chemoradiation improves the prognosis of patients with local recurrence, in particular those for which recurrence occurs shortly after initial treatment.
Prevention and Control of African Swine Fever in the Smallholder Pig Value Chain in Northern Uganda: Thematic Analysis of Stakeholders' Perceptions
Authors: Aliro T. , Chenais E. , Odongo W. , Okello D. M. , Masembe C. , Ståhl K.
Published: January 2022

African swine fever (ASF) is endemic in Uganda and considered a major constraint to pig production. In the absence of a vaccine, biosecurity is key for ASF prevention and control. To improve prevention and control on farm and community level there is need for more knowledge on current application of biosecurity practises, and better understanding of how pig value chain actors perceive prevention and control. To achieve this, a qualitative interview study involving focus group discussions (FGD) was conducted with actors from the smallholder pig value chain in northern Uganda. Six villages were purposively selected based on previous outbreaks of ASF, preliminary perceived willingness to control ASF, and the representation of several different value chain actors in the village. Results indicated that biosecurity practises such as basic hygiene routines including safe carcass handling, minimising direct and indirect contacts between pigs or between pigs and people, trade restrictions and sharing of disease information were implemented in some of the villages. Thematic analysis based on grounded theory revealed six categories of data relating to ASF prevention and control. Together these categories form a logical framework including both enablers and hindrances for ASF prevention and control. In summary participants mostly had positive perceptions of ASF biosecurity, describing measures as effective. Participants further possessed knowledge of ASF and its transmission, some of which was in line with known scientific knowledge and some not. Nevertheless, participants were hindered from preventing and controlling ASF due to biosecurity costs and a need to prioritise family livelihood over disease transmission risks, incompatibility of current biosecurity practises with local culture, traditions and social contexts and finally lack of access to veterinarians or, occasionally, low-quality veterinary services. The constraints could be addressed by applying participatory processes in designing biosecurity measures to ensure better adaptation to local cultural and social contexts.
Mechanisms and Contextual Factors Affecting the Implementation of Animal Health Surveillance in Tanzania: A Process Evaluation
Authors: George Janeth , Häsler Barbara , Komba Erick V. G. , Rweyemamu Mark , Kimera Sharadhuli I. , Mlangwa James E. D.
Published: January 2022

A strong animal health surveillance system is an essential determinant of the health of animal and human population. To ensure its functionality and performance, it needs to be evaluated regularly. Therefore, a process evaluation was conducted in this study to assess animal health surveillance processes, mechanisms and the contextual factors which facilitate or hinder uptake, implementation and sustainability of the system in Tanzania. A mixed-method study design was used to evaluate the national animal health surveillance system guided by a framework for process evaluation of complex interventions developed by Moore and others. The system was assessed against standard guidelines and procedures using the following attributes: fidelity, adherence, exposure, satisfaction, participation rate, recruitment and context. Quantitative and qualitative data were collected using a cross-sectional survey, key informant interviews, document review, site visits and non-participant observation. Data from questionnaires were downloaded, cleaned and analyzed in Microsoft™ Excel. Qualitative data were analyzed following deductive thematic and content analysis methods. Fidelity attribute showed that case identification is mainly based on clinical signs due to limited laboratory services for confirmation. Data collection was not well-coordinated and there were multiple disparate reporting channels. Adherence in terms of the proportion of reports submitted per month was only 61% of the target. District-level animal health officials spent an average of 60% of their weekly time on surveillance-related activities, but only 12% of them were satisfied with the surveillance system. Their dissatisfaction was caused by large area coverage with little to no facilitation, poor communication, and lack of a supporting system. The cost of surveillance data was found to be 1.4 times higher than the annual surveillance budget. The timeliness of the system ranged between 0 and 153 days from the observation date (median = 2 days, mean = 6 days). The study pointed out some deviations in animal health surveillance processes from the standard guidelines and their implication on the system's performance. The system could be improved by developing a user-friendly unified reporting system, the active involvement of subnational level animal health officials, optimization of data sources and an increase in the horizon of the financing mechanism.
Development of an Indirect ELISA to Detect African Swine Fever Virus pp62 Protein-Specific Antibodies
Authors: Zhong Kexin , Zhu Mengmeng , Yuan Qichao , Deng Zhibang , Feng Simeng , Liu Daoxin , Yuan Xiaomin
Published: January 2022

African swine fever (ASF) is a highly detrimental viral disease caused by African swine fever virus (ASFV). The occurrence and prevalence of this disease have become a serious threat to the global swine industry and national economies. At present, the detection volume of African swine fever is huge, more sensitive and accurate detection techniques are needed for the market. pp62 protein, as a protein in the late stage of infection, has strong antigenicity and a high corresponding antibody titer in infected pigs. In this study, the CP530R gene was cloned into expression vector pET-28a to construct a prokaryotic expression plasmid, which was induced by IPTG to express soluble pp62 protein. Western blot analysis showed that it had great reactivity. Using the purified recombinant protein as an antigen, an indirect ELISA method for detecting ASFV antibody was established. The method was specific only to ASFV-positive serum, 1:1600 diluted positive serum could still be detected, and the coefficients of variation (CV) of the intra assay and inter assay were both <10%. It turns out that the assays had excellent specificity, sensitivity, and repeatability. This provides an accurate, rapid, and economical method for the detection of ASFV antibody in clinical pig serum samples.
Bioassay Guided Fractionation of Senna singueana and Its Potential for Development of Poultry Phytogenic Feed Additives
Authors: Jambwa Prosper , Makhubu Fikile N. , Matope Gift , Fouche Gerda , McGaw Lyndy J.
Published: January 2022

There has been burgeoning interest in plant-based feed additives following restrictions placed on the use of antibiotic feed additives in many countries. Phytogenic feed additives are recommended to have a range of useful properties to support the growth and development of poultry to a similar level as that obtained by supplementing feed with antibiotics. The aim of this study was to evaluate the antibacterial, anti-lipoxygenase and antioxidant activity, and in vitro safety of fractions and isolated compounds from leaves of Senna singueana. Antibacterial activities of the fractions and isolated compounds were determined against a panel of bacteria using a two-fold serial microdilution assay and qualitative bioautography assays. Anti-lipoxygenase activity was evaluated using the ferrous oxidation-xylenol orange (FOX) method. Antioxidant activity was assessed qualitatively and quantitatively using radical scavenging assays. Dichloromethane and ethyl acetate fractions from solvent-solvent partitioning had the best antibacterial activity with MIC values ranging from 156 to 313 μg/ml. Fractions obtained from column chromatography had significant to weak antibacterial activity with MIC values ranging from 50 to 1,250 μg/ml. Bioautography showed clear bands of bacterial inhibition, indicating the presence of a number of active compounds in several fractions. The ethyl acetate fraction and all the tested column fractions had potent anti-lipoxygenase activity with IC50 values of ≤2.5 μg/ml which were lower than that of quercetin (positive control), indicating anti-inflammatory potential. The ethyl acetate fraction and several column fractions had powerful antioxidant activity with IC50 values of ≤5 μg/ml in the ABTS assay. Cytotoxicity values against Vero kidney cells ranged from LC50 = 40.0–989.3 μg/ml. Bioassay-guided fractionation led to the isolation and identification of a known bioactive compound, luteolin. S. singueana is a promising candidate for the development of poultry phytogenic feed additives.
MiR-21 in Lung Transplant Recipients With Chronic Lung Allograft Dysfunction
Authors: Miyahara Naofumi , Benazzo Alberto , Oberndorfer Felicitas , Iwasaki Akinori , Laszlo Viktoria , Döme Balasz , Hoda Mir Ali , Jaksch Peter , Klepetko Walter , Hoetzenecker Konrad
Published: January 2022

Background: Micro-RNA-21 (miR-21) is a post-translational regulator involved in epithelial-to-mesenchymal transition (EMT). Since EMT is thought to contribute to chronic lung allograft dysfunction (CLAD), we aimed to characterize miR-21 expression and distinct EMT markers in CLAD.

Methods: Expression of miR-21, vimentin, Notch intracellular domain (NICD) and SMAD 2/3 was investigated in explanted CLAD lungs of patients who underwent retransplantation. Circulating miR-21 was determined in collected serum samples of CLAD and matched stable recipients.

Results: The frequency of miR-21 expression was higher in restrictive allograft syndrome (RAS) than in bronchiolitis obliterans syndrome (BOS) specimens (86 vs 30%, p = 0.01); Vimentin, NICD and p-SMAD 2/3 were positive in 17 (100%), 12 (71%), and 7 (42%) BOS patients and in 7 (100%), 4 (57%) and 4 (57%) RAS cases, respectively. All four markers were negative in control tissue from donor lungs. RAS patients showed a significant increase in serum concentration of miR-21 over time as compared to stable recipients (p = 0.040).

Conclusion: To the best of our knowledge this is the first study highlighting the role miR-21 in CLAD. Further studies are necessary to investigate the involvement of miR-21 in the pathogenesis of CLAD and its potential as a therapeutic target.
Travel Bubbles in Air Transportation: Myth or Reality?
Authors: Sun Xiaoqian , Wandelt Sebastian , Zhang Anming
Published: January 2022

Aviation has been hit hard by COVID-19, with passengers stranded in remote destinations, airlines filing for bankruptcy, and uncertain demand scenarios for the future. Travel bubbles are discussed as one possible solution, meaning countries which have successfully constrained the spread of COVID-19 gradually increase their mutual international flights, returning to a degree of normality. This study aims to answer the question of whether travel bubbles are indeed observable in flight data for the year 2020. We take the year 2019 as reference and then search for anomalies in countries’ flight bans and recoveries, which could possibly be explained by having successfully implemented a travel bubble. To the best of our knowledge, this study is the first to try to address the identification of COVID-19 travel bubbles in real data. Our methodology and findings lead to several important insights regarding policy making, problems associated with the concept of travel bubbles, and raise interesting avenues for future research.
Harnessing the Phytase Production Potential of Soil-Borne Fungi from Wastewater Irrigated Fields Based on Eco-Cultural Optimization under Shake Flask Method
Authors: Sadaf Naila , Haider Muhammad Zulqurnain , Iqbal Naeem , Abualreesh Muyassar H. , Alatawi Aishah
Published: January 2022

Indigenous fungi present in agricultural soils could have synchronized their inherent potentials to the local climatic conditions. Therefore, the fungi resident in the untreated wastewater irrigated agricultural field might develop their potential for producing various enzymes to handle the induced full organic load from domestic wastewater and toxic chemicals from the textile industry. Around 53 various fungal isolates were grown and separated from the soil samples from these sites through soil dilution, soil-culture plate, and soil-culture plate methods. All the purified fungi were subjected to a phosphatase production test, and only 13 fungal strains were selected as phosphatase producers. Among them, only five fungi identified as Aspergillus niger, Aspergillus flavus, Aspergillus fumigatus, Penicillium purourogenum, and Mucor rouxii based on morphological similarities, showing higher phosphate solubilizing indices, were utilized for eco-cultural fine-tuning to harness their full production potential under shake flask (SF) method. Among various media, orchestral tuning, 200 µM sodium phytate as substrate with 1.5 mL of inoculum size of the fungi, pH 7, temperature 30 °C, glucose, and ammonium nitrate as carbon and nitrogen additive with seven days of incubation were found to be the most appropriate cultural conditions to harness the phytase production potential of the selected fungi. Aspergillus niger and Aspergillus flavus showed initial phytase activity (5.2 Units/mL, 4.8 Units/mL) and phytase specific activity (2.85, 2.65 Units/mL per mg protein) during screening to be enhanced up to 17 ± 0.033 (Units/mL), 16 ± 0.033 (Units/mL) and (13 ± 0.012), 10 ± 0.066 (Units/mL per mg protein), respectively, with the above-mentioned conditions. The phytase enzyme produced from these fungi were found to be almost stable for a wide range of pH (4–8); temperature (20–60 °C); insensitive to Ca2+ and Mg2+ ions, and EDTA, Ni2+, and Ba2+ inhibitors but highly sensitive to Mn2+, Cu2+, and Zn2+ ions, and Co2+, Cr3+, Al3+, Fe2+ and Ag1+ inhibitors. It was suggested that both phytase-producing strains of A. niger and A. flavus or their crude phytase enzymes might be good candidates for application in soils to release phosphates from phytate and a possible valuable substitute of phosphate fertilizers.
Impact of Inorganic Metal (Ag, Cu) Nanoparticles on the Quality of Seeds and Dried Rapeseed Sprouts
Authors: Kachel Magdalena , Rudy Stanisław , Krajewska Marta , Rudy Mariusz
Published: January 2022

The present study focuses on the impact of copper and silver nanoparticles on the chemical composition and physical properties of rapeseeds and rape sprouts. The seeds and sprouts were obtained from winter rape grown in a three-year cultivation (2018–2020) treated with silver (AgNP) and copper (CuNP) nanoparticles. In addition, the effect of the freeze-drying temperature (20; 40; 60 °C) on selected properties of the sprouts was studied. Spraying growing plants with nanoparticles resulted, in most cases, and depending on the year, in a reduction in the mass of seeds (MTS) by 9.5% (single nanoparticles spray ×1 CuNP in 2018), an increase in the fat content (by 8.80% for ×1 CuNP in 2018), a reduction in the protein content (by 12.93% for ×1 CuNP in 2018) and flavonoid content (by up to 58% for ×1 AgNP and CuNP in 2018), as well as increase in the glucosinolates content by 25% (for double nanoparticles spray ×2 AgNP in 2019). For the sprouts obtained from the rapeseeds, in most cases, a decrease in the content of flavonoids was observed (26.68% for ×1 AgNP; 20 °C in 2018), depending on the year of cultivation, the nanoparticles used, and the drying temperature. The obtained results remain inconclusive, which encourages the authors to undertake further research.
The Effects of Suaeda salsa/Zea mays L. Intercropping on Plant Growth and Soil Chemical Characteristics in Saline Soil
Authors: Wang Shoule , Zhao Zhenyong , Ge Shaoqing , Zhang Ke , Tian Changyan , Mai Wenxuan
Published: January 2022

Halophytes possess the capacity to uptake high levels of salt through physiological processes and their root architecture. Here, we investigated whether halophyte/non-halophyte intercropping in saline soil benefits plant growth and contains root-dialogue between interspecific species. Field and pot experiments were conducted to determine the plant biomasses and salt and nutrient distributions in three suaeda (Suaeda salsa)/maize (Zea mays L.) intercropping systems, set up by non-barrier, nylon-barrier, and plastic-barrier between plant roots. The suaeda/maize intercropping obviously transferred more Na+ to the suaeda root zone and decreased salt and Na+ contents. However, the biomass of the non-barrier-treated maize was significantly lower than that of the nylon and plastic barrier-treated maize. There was lower available N content in the soil of the non-barrier treated groups compared with the plastic barrier-treated groups. In addition, the pH was lower, and the available nutrient content was higher in the nylon barrier, which suggested that rhizospheric processes might occur between the two species. Therefore, we concluded that the suaeda/maize intercropping would be beneficial to the salt removal, but it caused an adverse effect for maize growth due to interspecific competition, and also revealed potential rhizospheric effects through the role of roots. This study provides an effective way for the improvement of saline land.
Resistance to Leaf and Yellow Rust in a Collection of Spanish Bread Wheat Landraces and Association with Ecogeographical Variables
Authors: Martínez-Moreno Fernando , Giraldo Patricia , Nieto Cristina , Ruiz Magdalena
Published: January 2022

A collection of 84 bread wheat Spanish landraces were inoculated with three isolates of leaf rust and one of yellow rust at the seedling stage in controlled conditions. The latency period of leaf rust on the susceptible landraces was also assessed. An extended collection of 149 landraces was planted in three locations in field trials to evaluate the naturally occurring leaf and yellow rust severity. Several landraces (36) were resistant to one leaf rust isolate at the seedling stage, but only one was resistant to all three isolates. Landraces resistant to PG14 leaf rust isolate originated from areas with higher precipitation and more uniform temperatures. Many resistant landraces were from the north-west zone of Spain, a region with high precipitation and uniform temperatures. Results from the field trials also confirmed this trend. Landraces from the north-west also possessed a longer latency period of leaf rust, an important component of partial resistance. Regarding yellow rust, 16 landraces showed a lower disease severity in the seedling tests. Again, the resistant landraces mostly originated from areas with higher precipitation (especially in winter) and more uniform temperature.
Empower Innovations in Routine Soil Testing
Authors: Reijneveld Jan Adriaan , van Oostrum Martijn Jasper , Brolsma Karst Michiel , Fletcher Dale , Oenema Oene
Published: January 2022

Conventional soil tests are commonly used to assess single soil characteristics. Thus, many different tests are needed for a full soil fertility/soil quality assessment, which is laborious and expensive. New broad-spectrum soil tests offer the potential to assess many soil characteristics quickly, but often face challenges with calibration, validation, and acceptance in practice. Here, we describe the results of a 20 year research program aimed at overcoming the aforementioned challenges. A three-step approach was applied: (1) selecting and establishing two contrasting rapid broad-spectrum soil tests, (2) relating the results of these new tests to the results of conventional soil tests for a wide variety of soils, and (3) validating the results of the new soil tests through field trials and communicating the results. We selected Near Infrared Spectroscopy (NIRS) and multi-nutrient 0.01 M CaCl2 extraction (1:10 soil to solution ratio; w/v) as broad-spectrum techniques. NIRS was extensively calibrated and validated for the physical, chemical, and biological characteristics of soil. The CaCl2 extraction technique was extensively calibrated and validated for ‘plant available’ nutrients, often in combination with the results of NIRS. The results indicate that the accuracy of NIRS determinations is high for SOM, clay, SOC, ECEC, Ca-CEC, N-total, sand, and inorganic-C (R2 ≥ 0.95) and good for pH, Mg-CEC, and S-total (R2 ≥ 0.90). The combination of the CaCl2 extraction technique and NIRS gave results that related well (R2 > 0.80) to the results of conventional soil tests for P, K, Mg, Na, Mn, Cu, Co, and pH. In conclusion, the three-step approach has revolutionized soil testing in The Netherlands. These two broad-spectrum soil tests have improved soil testing; have contributed to increased insights into the physical, chemical, and biological characteristics of soil; and have thereby led to more sustainable soil management and cropping systems.
Toxicity Evaluation of Selected Plant Water Extracts on a Honey Bee (Apis mellifera L.) Larvae Model
Authors: Kruszakin Roksana , Migdal Paweł
Published: January 2022

So far, larval rearing in vitro has been an important method in the assessment of bee toxicology, particularly in pesticide risk assessment. However, natural products are increasingly used to control honey bee pathogens or to enhance bee immunity, but their effects on honey bee larvae are mostly unknown. In this study, laboratory studies were conducted to determine the effects of including selected aqueous plant infusions in the diet of honey bee (Apis mellifera L.) larvae in vitro. The toxicity of infusions from three different plant species considered to be medicinal plants was evaluated: tansy (Tanacetum vulgare L.), greater celandine (Chelidonium majus L.), and coriander (Coriandrum sativum L.). The impact of each on the survival of the larvae of honey bees was also evaluated. One-day-old larvae were fed a basal diet consisting of distilled water, sugars (glucose and fructose), yeast extract, and freeze-dried royal jelly or test diets in which distilled water was replaced by plant infusions. The proportion of the diet components was adjusted to the age of the larvae. The larvae were fed twice a day. The experiment lasted seven days. Significant statistical differences in survival rates were found between groups of larvae (exposed or not to the infusions of tansy, greater celandine, and coriander). A significant decrease (p < 0.05) in the survival rate was observed in the group with the addition of a coriander herb infusion compared to the control. These results indicate that plant extracts intended to be used in beekeeping should be tested on all development stages of honey bees.
Selective Inner Hair Cell Loss in a Neonate Harbor Seal (Phoca vitulina)
Authors: Morell Maria , Rojas Laura , Haulena Martin , Busse Björn , Siebert Ursula , Shadwick Robert E. , Raverty Stephen A.
Published: January 2022

Congenital hearing loss is recognized in humans and other terrestrial species. However, there is a lack of information on its prevalence or pathophysiology in pinnipeds. It is important to have baseline knowledge on marine mammal malformations in the inner ear, to differentiate between congenital and acquired abnormalities, which may be caused by infectious pathogens, age, or anthropogenic interactions, such as noise exposure. Ultrastructural evaluation of the cochlea of a neonate harbor seal (Phoca vitulina) by scanning electron microscopy revealed bilateral loss of inner hair cells with intact outer hair cells. The selective inner hair cell loss was more severe in the basal turn, where high-frequency sounds are encoded. The loss of inner hair cells started around 40% away from the apex or tip of the spiral, reaching a maximum loss of 84.6% of hair cells at 80–85% of the length from the apex. Potential etiologies and consequences are discussed. This is believed to be the first case report of selective inner hair cell loss in a marine mammal neonate, likely congenital.
Influence of the Zinc and Fibre Addition in the Diet on Biomechanical Bone Properties in Weaned Piglets
Authors: Skiba Grzegorz , Raj Stanisława , Sobol Monika , Kowalczyk Paweł , Barszcz Marcin , Taciak Marcin , Tuśnio Anna , Čobanová Klaudia , Grešáková Ľubomira , Grela Eugeniusz Ryszard
Published: January 2022

The effects of the zinc and fibre source in piglets’ diet on the bone mineral content, density, and strength parameters of the femur were investigated using 24 piglets fed a diet supplemented with either lignocellulose (LC) or potato fibre (PF). Half of each group of piglets consumed a diet with ZnSO4 monohydrate or with zinc glycinate (ZnGly). The diets contained similar amounts of lysine, energy, and fibre. Bone mineral content and density were over 9% higher in pigs receiving diets with ZnGly than in animals fed diets with ZnSO4. Moreover, ZnGly strongly improved maximum and elastic strength (by 25.7 and 20.0%, respectively, p < 0.0001) and bone stiffness (by 29.4%, p < 0.0001). Only the mass of the femur was affected by the type of fibre in the diet, as the femurs of piglets fed diets with LC were over 7% (p < 0.0001) heavier than in piglets fed diets with PF. The intake of digestible zinc and the zinc content in the blood serum were positively correlated with the measured bone parameters and, depending on the parameter, “r” ranged from 0.749 to 0.866 and from 0.400 to 0.479, respectively. It can be concluded that bone parameters are affected more strongly by the organic than inorganic source of zinc.
Influence of Broiler Age on the Apparent Metabolizable Energy of Cereal Grains Determined Using the Substitution Method
Authors: Khalil Mahmoud M. , Abdollahi Mohammad Reza , Zaefarian Faegheh , Chrystal Peter V. , Ravindran Velmurugu
Published: January 2022

The present study investigated the influence of broiler age on the AMEn of wheat, sorghum, barley, and corn using the substitution method at six different ages (days 7, 14, 21, 28, 35, and 42). A corn-soybean meal basal diet was formulated and, the test diets were developed by replacing (w/w) 300 g/kg of the basal diet with wheat, sorghum, barley, or corn. Bird age influenced (p < 0.001) the AMEn of wheat and sorghum but had no effect (p > 0.05) on those of barley and corn. The AMEn of wheat increased with age (p < 0.001) from 12.53 MJ/kg DM in week 1 to 14.55 MJ/kg DM in week 2, then declined subsequently, but no linear or quadratic responses were observed. The AMEn of sorghum demonstrated a quadratic response (p < 0.05), increasing from 12.84 MJ/kg DM in week 1 to 13.95 MJ/kg DM in week 2, and then plateauing to week 6. Overall, the present results suggest that the effect of broiler age on the AMEn varies depending on the grain type. The current data suggest that the application of age-dependent AME or AMEn of wheat and sorghum will lead to more precise feed formulations.
Gene-Set Enrichment Analysis for Identifying Genes and Biological Activities Associated with Growth Traits in Dromedaries
Authors: Sani Morteza Bitaraf , Roudbari Zahra , Karimi Omid , Banabazi Mohammad Hossein , Esmaeilkhanian Saeid , Asadzadeh Nader , Zare Harofte Javad , Shafei Naderi Ali , Burger Pamela Anna
Published: January 2022

Growth is an important heritable economic trait for dromedaries and necessary for planning a successful breeding program. Until now, genome-wide association studies (GWAS) and QTL-mapping have identified significant single nucleotide polymorphisms (SNPs) associated with growth in domestic animals, but in dromedaries, the number of studies is very low. This project aimed to find biological themes affecting growth in dromedaries. In the first step, 99 candidate SNPs were chosen from a previously established set of SNPs associated with body weight, gain, and birth weight in Iranian dromedaries. Next, 0.5 kb upstream and downstream of each candidate SNP were selected from NCBI (assembly accession: GCA_000803125.3). The annotation of fragments with candidate SNPs regarding the reference genome was retrieved using the Blast2GO tool. Candidate SNPs associated with growth were mapped to 22 genes, and 25 significant biological themes were identified to be related to growth in dromedaries. The main biological functions included calcium ion binding, protein binding, DNA-binding transcription factor activity, protein kinase activity, tropomyosin binding, myosin complex, actin-binding, ATP binding, receptor signaling pathway via JAK-STAT, and cytokine activity. EFCAB5, MTIF2, MYO3A, TBX15, IFNL3, PREX1, and TMOD3 genes are candidates for improving growth in camel breeding programs.
Current Consumer Perceptions of Animal Welfare across Different Farming Sectors on the Island of Ireland
Authors: Sweeney Sharon , Regan Áine , McKernan Claire , Benson Tony , Hanlon Alison , Dean Moira
Published: January 2022

There has been increased public interest and concerns in issues such as farm animal welfare (FAW) on the island of Ireland, stoked in part by political and governance changes, such as Brexit and COVID-19. Front-of-pack food labelling represents a primary information channel for many people. In advance of considering formalised food labelling schemes, specifically relating to FAW, it is important to ensure an up-to-date understanding of current consumer perceptions of FAW. With this aim, the current study utilised a mixed methodology. Nine focus group discussions (n = 41) and an online survey (n = 972) with food consumers in Ireland and Northern Ireland explored perceptions of FAW. Results suggest that overall perceptions of FAW are high, and consumers perceive FAW to have improved in the last decade. Quantitative (ANOVA) and qualitative results show variations in perception of FAW between sectors. Results from the focus group discussions identified factors underlying consumers’ perception of FAW: the living conditions of the animal, size and intensity of the farm, national standards and schemes, and visibility. Information insufficiencies and knowledge gaps were identified. The findings are discussed in relation to policy implications for the role of public engagement, front-of-pack welfare labelling, and quality assurance schemes.
Context-Dependent Gestural Laterality: A Multifactorial Analysis in Captive Red-Capped Mangabeys
Authors: Aychet Juliette , Monchy Noémie , Blois-Heulin Catherine , Lemasson Alban
Published: January 2022

Catarrhine primates gesture preferentially with their right hands, which led to the hypothesis of a gestural origin of human left-hemispheric specialization for language. However, the factors influencing this gestural laterality remain understudied in non-hominoid species, particularly in intraspecific contexts, although it may bring valuable insights into the proximate and ultimate causes of language lateralization. We present here a preliminary investigation of intraspecific gestural laterality in catarrhine monkeys, red-capped mangabeys (Cercocebus torquatus). We described the spontaneous production of brachio-manual intentional gestures in twenty-five captive subjects. Although we did not evidence any significant gestural lateralization neither at the individual- nor population-level, we found that mangabeys preferentially use their right hands to gesture in negative social contexts, such as aggressions, suggesting an effect of emotional lateralization, and that they adapt to the position of their receiver by preferentially using their ipsilateral hand to communicate. These results corroborate previous findings from ape studies. By contrast, factors related to gesture form and socio-demographic characteristics of signaler and receiver did not affect gestural laterality. To understand better the relationships between gestural laterality and brain lateralization from an evolutionary perspective, we suggest that the gestural communication of other monkey species should be examined with a multifactorial approach.
Herpetic Pneumonia in Indian Ringneck Parrots (Psittacula krameri): First Report of Novel Psittacid Alphaherpesvirus-5 Infection in Europe
Authors: Bottinelli Marco , Fortin Andrea , Zanardello Claudia , Budai Jane , Gobbo Federica , Antonazzo Gianmaria , Leone Stefania , Merenda Marianna , Terregino Calogero , Catania Salvatore
Published: January 2022

The first two European outbreaks of herpetic pneumonia caused by Psittacid alphaherpesvirus-5 were diagnosed based on gross pathology findings, histological examination, transmission electron microscopy visualization and genome sequencing. The outbreaks, characterized by high morbidity and high mortality rates, involved two parrot species, namely the Indian ringneck parrot (Psittacula krameri) and the Alexandrine parakeet (Psittacula eupatria). Clinical signs observed were ruffled feathers, dyspnea, tail bobbing, open wings while breathing, depression and anorexia. Necropsy was performed on Indian ringneck parrots only, and the most evident and serious gross lesion found in all the birds was a diffuse marked consolidation of the lungs associated with parenchyma congestion and oedema. Histological examination confirmed the existence of bronchopneumonia characterized by the presence of syncytial cells with intranuclear inclusion bodies. In one bird, fibrinous airsacculitis was observed as well. Lung tissue inspection through electron microscopy revealed the presence of virus particles resembling herpesviruses. Viral DNA was extracted, amplified using primers for Alloherpesviridae DNA polymerase gene detection, and then sequenced. BLAST analysis showed a 100% identity with the only previously reported sequence of PsHV-5 (MK955929.1).
Trait-Based Vaccination of Individual Meerkats (Suricata suricatta) against Tuberculosis Provides Evidence to Support Targeted Disease Control
Authors: Patterson Stuart J. , Clutton-Brock Tim H. , Pfeiffer Dirk U. , Drewe Julian A.
Published: January 2022

Individuals vary in their potential to acquire and transmit infections, but this fact is currently underexploited in disease control strategies. We trialled a trait-based vaccination strategy to reduce tuberculosis in free-living meerkats by targeting high-contact meerkats (socially dominant individuals) in one study arm, and high-susceptibility individuals (young subordinates) in a second arm. We monitored infection within vaccinated groups over two years comparing the results with untreated control groups. Being a member of a high-contact group had a protective effect on individuals’ survival times (Hazard Ratio = 0.5, 95% Confidence Interval, CI: 0.29–0.88, p = 0.02) compared to control groups. Over the study, odds of testing positive for tuberculosis increased more than five-fold in control groups (Odds Ratio = 5.40, 95% CI = 0.94–30.98, p = 0.058); however, no increases were observed in either of the treatment arms. Targeted disease control approaches, such as the one described in this study, allow for reduced numbers of interventions. Here, trait-based vaccination was associated with reduced infection rates and thus has the potential to offer more efficient alternatives to traditional mass-vaccination policies. Such improvements in efficiency warrant further study and could make infectious disease control more practically achievable in both animal (particularly wildlife) and human populations.
Canine Seventh Lumbar Vertebra Fracture: A Systematic Review
Authors: Caterino Chiara , Aragosa Federica , Della Valle Giovanni , Fatone Gerardo
Published: January 2022

(1) Background: Lumbosacral traumatic injuries are reported as 39% of canine vertebral lesions. This area is prone to fracture and luxation. Several surgical techniques were described from 1975 to 2021 to stabilize the traumatic injuries of the lumbosacral junction. This report aims to critically review the available literature focused on clinical presentation, surgical techniques, and follow-up of the lumbar vertebra fracture. (2) Methods: Three bibliographic databases: PubMed, Google Scholar, and Scopus were used with a board search of Lumbosacral junction fracture AND, of L7 fracture AND (canine OR dog). The Joanna Briggs Institute (JBI) Critical Appraisal Checklist for case reports and case series was applied for the studies included. (3) Results: A total of 432 reports yielded only nine that met the inclusion criteria. Non-ambulatory paraparesis/plegia, sciatic nerve involvement, faecal/urinary incontinence, and severe back lumbar pain were the most reported signs. Survey radiographs were the most reported technique to confirm the diagnoses. The surgical treatment was reported in all reports examined with a good long-term prognosis. (4) Conclusions: The seventh lumbar vertebra fracture, despite the different surgical techniques performed, had a favourable prognosis for long-term outcome and neurological recovery.
Chemical Stability of Ascorbic Acid Integrated into Commercial Products: A Review on Bioactivity and Delivery Technology
Authors: Yin Xin , Chen Kaiwen , Cheng Hao , Chen Xing , Feng Shuai , Song Yuanda , Liang Li
Published: January 2022

The L-enantiomer of ascorbic acid is commonly known as vitamin C. It is an indispensable nutrient and plays a key role in retaining the physiological process of humans and animals. L-gulonolactone oxidase, the key enzyme for the de novo synthesis of ascorbic acid, is lacking in some mammals including humans. The functionality of ascorbic acid has prompted the development of foods fortified with this vitamin. As a natural antioxidant, it is expected to protect the sensory and nutritional characteristics of the food. It is thus important to know the degradation of ascorbic acid in the food matrix and its interaction with coexisting components. The biggest challenge in the utilization of ascorbic acid is maintaining its stability and improving its delivery to the active site. The review also includes the current strategies for stabilizing ascorbic acid and the commercial applications of ascorbic acid.