bluuebunny/crossref_metadata_2025 huggingface dataset:
Sex Differences in Cerebral Small Vessel Disease: A Systematic Review and Meta-Analysis
Authors: Jiménez-Sánchez Lorena , Hamilton Olivia K. L. , Clancy Una , Backhouse Ellen V. , Stewart Catriona R. , Stringer Michael S. , Doubal Fergus N. , Wardlaw Joanna M.
Published: October 2021

Background:Cerebral small vessel disease (SVD) is a common cause of stroke, mild cognitive impairment, dementia and physical impairments. Differences in SVD incidence or severity between males and females are unknown. We assessed sex differences in SVD by assessing the male-to-female ratio (M:F) of recruited participants and incidence of SVD, risk factor presence, distribution, and severity of SVD features.

Methods:We assessed four recent systematic reviews on SVD and performed a supplementary search of MEDLINE to identify studies reporting M:F ratio in covert, stroke, or cognitive SVD presentations (registered protocol: CRD42020193995). We meta-analyzed differences in sex ratios across time, countries, SVD severity and presentations, age and risk factors for SVD.

Results:Amongst 123 relevant studies (n= 36,910 participants) including 53 community-based, 67 hospital-based and three mixed studies published between 1989 and 2020, more males were recruited in hospital-based than in community-based studies [M:F = 1.16 (0.70) vs. M:F = 0.79 (0.35), respectively;p< 0.001]. More males had moderate to severe SVD [M:F = 1.08 (0.81) vs. M:F = 0.82 (0.47) in healthy to mild SVD;p< 0.001], and stroke presentations where M:F was 1.67 (0.53). M:F did not differ for recent (2015–2020) vs. pre-2015 publications, by geographical region, or age. There were insufficient sex-stratified data to explore M:F and risk factors for SVD.

Conclusions:Our results highlight differences in male-to-female ratios in SVD severity and amongst those presenting with stroke that have important clinical and translational implications. Future SVD research should report participant demographics, risk factors and outcomes separately for males and females.

Systematic Review Registration:[PROSPERO], identifier [CRD42020193995].
Spatial Distribution of Immune Cells in Head and Neck Squamous Cell Carcinomas
Authors: Idel Christian , Ribbat-Idel Julika , Klapper Luise , Krupar Rosemarie , Bruchhage Karl-Ludwig , Dreyer Eva , Rades Dirk , Polasky Christina , Offermann Anne , Kirfel Jutta , Perner Sven , Wollenberg Barbara
Published: October 2021

Background

Head and neck squamous cell carcinomas (HNSCCs) have a very moderate response rate to immune checkpoint inhibitor (ICI) treatment compared to other cancer types. Lacking predictive markers for treatment response, we analyzed the immune status of HNSCC and assessed the spatial distribution of immune cells.

Materials and Methods

Via assessing hematoxylin–eosin (H&E) stains, we divided HNSCCs by the immune cell distribution in hot, cold, and excluded tumors. For each group, each with 10 tumors, we performed serial immunohistochemical (IHC) staining of the immune cell markers, checkpoint molecules, and immune regulators.

Results

The spatial distributions were different for each immune cell type, allocating regulatory T cells (Tregs) and CD11b cells predominantly in the stroma. CD4 and CD8 cells were present either in the tumor stroma or between cancer cells. Interestingly, the expressions of PD-1 (programmed cell death 1 receptor) and PD-L1 (programmed death-ligand 1) were higher in hot tumors in comparison to cold and excluded tumors. The expression of pSMAD [indicating active transforming growth factor beta (TGF-β)] was higher in excluded tumors.

Conclusion

Different immune cell distribution patterns within tumors might be crucial for ICI treatment response since hot tumors have the highest expressions of PD-1 and PD-L1. TGF-β might be a key regulator for immune cell distribution and a promising therapeutic target that determines the formation of hot or excluded immune patterns.
Classification and Diagnosis of Residual Thyroid Tissue in SPECT Images Based on Fine-Tuning Deep Convolutional Neural Network
Authors: Guo Yinxiang , Xu Jianing , Li Xiangzhi , Zheng Lin , Pan Wei , Qiu Meiting , Mao Shuyi , Huang Dongfei , Yang Xiaobo
Published: October 2021

Patients with thyroid cancer will take a small dose of 131I after undergoing a total thyroidectomy. Single-photon emission computed tomography (SPECT) is used to diagnose whether thyroid tissue remains in the body. However, it is difficult for human eyes to observe the specificity of SPECT images in different categories, and it is difficult for doctors to accurately diagnose the residual thyroid tissue in patients based on SPECT images. At present, the research on the classification of thyroid tissue residues after thyroidectomy is still in a blank state. This paper proposes a ResNet-18 fine-tuning method based on the convolutional neural network model. First, preprocess the SPECT images to improve the image quality and remove background interference. Secondly, use the preprocessed image samples to fine-tune the pretrained ResNet-18 model to obtain better features and finally use the Softmax classifier to diagnose the residual thyroid tissue. The method has been tested on SPECT images of 446 patients collected by local hospital and compared with the widely used lightweight network SqueezeNet model and ShuffleNetV2 model. Due to the small data set, this paper conducted 10 random grouping experiments. Each experiment divided the data set into training set and test set at a ratio of 3:1. The accuracy and sensitivity rates of the model proposed in this paper are 96.69% and 94.75%, which are significantly higher than other models (p < 0.05). The specificity and precision rates are 99.6% and 99.96%, respectively, and there is no significant difference compared with other models. (p > 0.05). The area under the curve of the proposed model, SqueezeNet, and ShuffleNetv2 are 0.988 (95% CI, 0.941–1.000), 0.898 (95% CI, 0.819–0.951) (p = 0.0257), and 0.885 (95% CI, 0.803–0.941) (p = 0.0057) (p < 0.05). We prove that this thyroid tissue residue classification system can be used as a computer-aided diagnosis method to effectively improve the diagnostic accuracy of thyroid tissue residues. While more accurately diagnosing patients with residual thyroid tissue in the body, we try our best to avoid the occurrence of overtreatment, which reflects its potential clinical application value.
Creatinine-Based Formulae Poorly Match in the Classification of Hypofiltration or Hyperfiltration in a General Population of Adolescents: A Retrospective Analysis of a Cross-Sectional Study
Authors: Šebeková Katarína , Gurecká Radana , Tóthová Ĺubomíra , Podracká Ĺudmila
Published: October 2021

Pediatric formulae to estimate glomerular filtration rate (eGFR) give a broad range of values. Their consistency in assigning the subjects as hypofiltrating or hyperfiltrating is unknown. In 1993 apparently healthy adolescents (53.4% females) aged 14–17 years, we investigated the concordance of six creatinine-based formulae in the classification of the subjects into ≤ 5th or ≥95th percentile of eGFR, and the between-groups difference in the prevalence of cardiometabolic risk factors. Mean eGFR varied between 77 and 121 mL/min/1.73 m2. Arbitrary setting of hypofiltration or hyperfiltration to 5% returned 46 males and 53 females. At least one formula classified 89 males and 99 females as hypofiltrating and 105 males and 114 females as hyperfiltrating. All six formulae concordantly classified 15 males and 17 females as hypofiltrating and 9 and 14, respectively, as hyperfiltrating. Pairwise, formulae consistently classified hypofiltration in 42–87% of subjects with hyperfiltration in 28–94%. According to two out of the six formulae, hyperfiltration was associated with an increased prevalence of obesity and obesity-associated comorbidities. Hypofiltrating subjects did not manifest chronic kidney disease–associated comorbidities. Further studies in different populations of healthy adolescents are needed before it is possible to conclude which creatinine-based formula is appropriate for the classification of hypofiltration and hyperfiltration in nonclinical cohorts.
Results of WICOVIR Gargle Pool PCR Testing in German Schools Based on the First 100,000 Tests
Authors: Kheiroddin Parastoo , Schöberl Patricia , Althammer Michael , Cibali Ezgi , Würfel Thea , Wein Hannah , Kulawik Birgit , Buntrock-Döpke Heike , Weigl Eva , Gran Silvia , Gründl Magdalena , Langguth Jana , Lampl Benedikt , Judex Guido , Niggel Jakob , Pagel Philipp , Schratzenstaller Thomas , Schneider-Brachert Wulf , Gastiger Susanne , Bodenschatz Mona , Konrad Maike , Levchuk Artem , Roth Cornelius , Schöner David , Schneebauer Florian , Rohrmanstorfer René , Dekens Marcus P. , Brandstetter Susanne , Zuber Johannes , Wallerstorfer Daniel , Burkovski Andreas , Ambrosch Andreas , Wagner Thomas , Kabesch Michael
Published: October 2021

Background: Opening schools and keeping children safe from SARS-CoV-2 infections at the same time is urgently needed to protect children from direct and indirect consequences of the COVID-19 pandemic. To achieve this goal, a safe, efficient, and cost-effective SARS-CoV-2 testing system for schools in addition to standard hygiene measures is necessary.

Methods: We implemented the screening WICOVIR concept for schools in the southeast of Germany, which is based on gargling at home, pooling of samples in schools, and assessment of SARS-CoV-2 by pool rRT-PCR, performed decentralized in numerous participating laboratories. Depooling was performed if pools were positive, and results were transmitted with software specifically developed for the project within a day. Here, we report the results after the first 13 weeks in the project.

Findings: We developed and implemented the proof-of-concept test system within a pilot phase of 7 weeks based on almost 17,000 participants. After 6 weeks in the main phase of the project, we performed >100,000 tests in total, analyzed in 7,896 pools, identifying 19 cases in >100 participating schools. On average, positive children showed an individual CT value of 31 when identified in the pools. Up to 30 samples were pooled (mean 13) in general, based on school classes and attached school staff. All three participating laboratories detected positive samples reliably with their previously established rRT-PCR standard protocols. When self-administered antigen tests were performed concomitantly in positive cases, only one of these eight tests was positive, and when antigen tests performed after positive pool rRT-PCR results were already known were included, 3 out of 11 truly positive tests were also identified by antigen testing. After 3 weeks of repetitive WICOVIR testing twice weekly, the detection rate of positive children in that cohort decreased significantly from 0.042 to 0.012 (p = 0.008).

Interpretation: Repeated gargle pool rRT-PCR testing can be implemented quickly in schools. It is an effective, valid, and well-received test system for schools, superior to antigen tests in sensitivity, acceptance, and costs.
Routine Administration of a Multispecies Probiotic Containing Bifidobacterium and Lactobacillus to Very Low Birth Weight Infants Had No Significant Impact on the Incidence of Necrotizing Enterocolitis
Authors: Juber Brian A. , Boly Timothy J. , Pitcher Graeme J. , McElroy Steven J.
Published: October 2021

Background: Necrotizing enterocolitis (NEC) is the leading cause of gastrointestinal morbidity in preterm infants, and prevention and treatment strategies have remained largely unchanged over the past several decades. As understanding of the microbiome has increased, probiotics have been hypothesized as a possible strategy for decreasing rates of NEC, and several studies have noted significant decreases in rates of NEC after initiation of probiotics in preterm infants. However, a recent AAP report cited caution on the use of probiotic use in part because studies of probiotic use in ELBW infants are lacking. As our unit began routine use of probiotics for all infants <33 weeks in 2015 and we are a leading institution for intact survival of ELBW infants, we attempted to answer if probiotic use can impact the rate of NEC in VLBW and ELBW infants.

Methods: We conducted a single-center retrospective chart review of infants with modified Bell's stage ≥2a NEC for the 4 years prior to and 5 years after initiation of a protocol involving routine supplementation of a multispecies probiotic to premature infants at the University of Iowa, Stead Family Children's Hospital. The primary outcome measures were rates of modified Bell's stage ≥2a NEC and all-cause pre-discharge mortality at our institution before and after initiation of routine probiotic supplementation in 2015.

Results: In our institution, neither the rates of modified Bell's stage ≥2a NEC, nor the rates of all-cause mortality were significantly altered in very low birth weight (VLBW) infants by the initiation of routine probiotic use (NEC rates pre-probiotic 2.1% vs. post-probiotic 1.5%; all-cause mortality rates pre-probiotic 8.4% vs. post-probiotic 7.4%). Characteristics of our two cohorts were overall similar except for a significantly lower 5-minute APGAR score in infants in the post-probiotic epoch (pre-probiotic 8 vs. post-probiotic 6 p = 0.0316), and significantly more infants in the post-probiotic epoch received probiotics (pre-probiotics 0% vs. post-probiotics 65%; p < 0.0001). Similarly, probiotic use had no impact on the incidence of NEC when we restricted our data to only extremely low birth weight (ELBW) infants (pre-probiotics 1.6% vs post-probiotics 4.1%). When we restricted our analysis to only inborn infants, probiotics still had no impact on NEC rates in VLBW infants (1.5% pre- and 1.1% post-probiotic, p = 0.61) or ELBW infants (2% pre- and 2.1% post-probiotic, p = 0.99)

Conclusions: Contrary to other studies, we found no significant difference in rates of modified Bell's stage ≥2a NEC or all-cause pre-discharge mortality in VLBW infants following routine administration of a multispecies probiotic supplement.
The Trend of Targeted Therapies in Chinese Patients With Ankylosing Spondylitis: Results From a Real-Life Survey
Authors: Wen Yiquan , Hu Zhuoran , Xie Baozhao , Yuan Fei , Xie Zhengquan , Jiang Yutong , Lin He , Qi Jun , Chen Qiyun
Published: October 2021

Introduction: Targeted medication, including mostly biologics and small-molecule chemical drugs, is an important therapy for ankylosing spondylitis (AS). There are still limited data on the preference of different targeted drugs in Chinese AS patients.

Methods: A questionnaire-based cross-sectional study was performed on AS patients from six hospitals in three provinces in South China. Anti-rheumatic diseases’ medication history includes the recent and previous usage of biologics or Janus kinase inhibitors (JAKi) in the last complete course of treatment, disease severity, and reasons for targeted-treatment change or preference.

Results: 354 of 366 participants responded to the online survey. The participants’ median age was 32 years, with a median of 7.3 years of disease duration; 79.7% were male. 63.6% of them were in the course of biologics or JAKi. Generic ETN is the most widely used and willing-to-use biologic though the proportion of its usage shrunk in the present compared with the past. The choice of original-branded ADA demonstrated an increase in usage. The preference of secukinumab and tofacitinib depicted a quick ascending trend.

Conclusion: TNF-α inhibitors (TNFi) are still the most popular targeted medication for AS in China. Their price influences patients’ preferences mostly. The doctor’s recommendation is also part of the equation. Rheumatologists should pay more attention to patients’ education to formulate targeted therapeutic plans.
Comparative Transcriptome Analysis in Homo- and Hetero-Grafted Cucurbit Seedlings
Authors: Bantis Filippos , Tsiolas George , Mouchtaropoulou Evangelia , Tsompanoglou Ioanna , Polidoros Alexios N. , Argiriou Anagnostis , Koukounaras Athanasios
Published: October 2021

Watermelon (Citrullus lanatus) is a valuable horticultural crop with nutritional benefits grown worldwide. It is almost exclusively cultivated as grafted scions onto interspecific squash rootstock (Cucurbita maxima × Cucurbita moschata) to improve the growth and yield and to address the problems of soilborne diseases and abiotic stress factors. This study aimed to examine the effect of grafting (homo- and hetero-grafting) on the transcriptome level of the seedlings. Therefore, we compared homo-grafted watermelon (WW) with non-grafted watermelon control (W), homo-grafted squash (SS) with non-grafted squash control (S), hetero-grafted watermelon onto squash (WS) with SS, and WS with WW. Different numbers of differentially expressed genes (DEGs) were identified in each comparison. In total, 318 significant DEGs were detected between the transcriptomes of hetero-grafts and homo-grafts at 16 h after grafting. Overall, a significantly higher number of downregulated transcripts was detected among the DEGs. Only one gene showing increased expression related to the cytokinin synthesis was common in three out of four comparisons involving WS, SS, and S. The highest number of differentially expressed (DE) transcripts (433) was detected in the comparison between SS and S, followed by the 127 transcripts between WW and W. The study provides a description of the transcriptomic nature of homo- and hetero-grafted early responses, while the results provide a start point for the elucidation of the molecular mechanisms and candidate genes for the functional analyses of hetero-graft and homo-graft systems in Cucurbitaceae and generally in the plants.
Lipid Peroxide-Derived Reactive Carbonyl Species as Mediators of Oxidative Stress and Signaling
Authors: Biswas Md. Sanaullah , Mano Jun’ichi
Published: October 2021

Oxidation of membrane lipids by reactive oxygen species (ROS) or O2/lipoxygenase leads to the formation of various bioactive compounds collectively called oxylipins. Reactive carbonyl species (RCS) are a group of oxylipins that have the α,β-unsaturated carbonyl structure, including acrolein and 4-hydroxy-(E)-2-nonenal. RCS provides a missing link between ROS stimuli and cellular responses in plants via their electrophilic modification of proteins. The physiological significance of RCS in plants has been established based on the observations that the RCS-scavenging enzymes that are overexpressed in plants or the RCS-scavenging chemicals added to plants suppress the plants’ responses to ROS, i.e., photoinhibition, aluminum-induced root damage, programmed cell death (PCD), senescence, abscisic acid-induced stomata closure, and auxin-induced lateral root formation. The functions of RCS are thus a key to ROS- and redox-signaling in plants. The chemical species involved in distinct RCS signaling/damaging phenomena were recently revealed, based on comprehensive carbonyl determinations. This review presents an overview of the current status of research regarding RCS signaling functions in plants and discusses present challenges for gaining a more complete understanding of the signaling mechanisms.
Drought Stress-Mediated Transcriptome Profile Reveals NCED as a Key Player Modulating Drought Tolerance in Populus davidiana
Authors: Lee Sang-Uk , Mun Bong-Gyu , Bae Eun-Kyung , Kim Jae-Young , Kim Hyun-Ho , Shahid Muhammad , Choi Young-Im , Hussain Adil , Yun Byung-Wook
Published: October 2021

Populus trichocarpa has been studied as a model poplar species through biomolecular approaches and was the first tree species to be genome sequenced. In this study, we employed a high throughput RNA-sequencing (RNA-seq) mediated leaf transcriptome analysis to investigate the response of four different Populus davidiana cultivars to drought stress. Following the RNA-seq, we compared the transcriptome profiles and identified two differentially expressed genes (DEGs) with contrasting expression patterns in the drought-sensitive and tolerant groups, i.e., upregulated in the drought-tolerant P. davidiana groups but downregulated in the sensitive group. Both these genes encode a 9-cis-epoxycarotenoid dioxygenase (NCED), a key enzyme required for abscisic acid (ABA) biosynthesis. The high-performance liquid chromatography (HPLC) measurements showed a significantly higher ABA accumulation in the cultivars of the drought-tolerant group following dehydration. The Arabidopsis nced3 loss-of-function mutants showed a significantly higher sensitivity to drought stress, ~90% of these plants died after 9 days of drought stress treatment. The real-time PCR analysis of several key genes indicated a strict regulation of drought stress at the transcriptional level in the P. davidiana drought-tolerant cultivars. The transgenic P. davidiana NCED3 overexpressing (OE) plants were significantly more tolerant to drought stress as compared with the NCED knock-down RNA interference (RNAi) lines. Further, the NCED OE plants accumulated a significantly higher quantity of ABA and exhibited strict regulation of drought stress at the transcriptional level. Furthermore, we identified several key differences in the amino acid sequence, predicted structure, and co-factor/ligand binding activity of NCED3 between drought-tolerant and susceptible P. davidiana cultivars. Here, we presented the first evidence of the significant role of NCED genes in regulating ABA-dependent drought stress responses in the forest tree P. davidiana and uncovered the molecular basis of NCED3 evolution associated with increased drought tolerance.
Flood-Rings Production Modulated by River Regulation in Eastern Boreal Canada
Authors: Nolin Alexandre F. , Tardif Jacques C. , Conciatori France , Bergeron Yves
Published: October 2021

In northeastern boreal Canada, the long-term perspective on spring flooding is hampered by the absence of long gage records. Changes in the tree-ring anatomy of periodically flooded trees have allowed the reconstruction of historical floods in unregulated hydrological systems. In regulated rivers, the study of flood rings could recover past flood history, assuming that the effects of hydrological regulation on their production can be understood. This study analyzes the effect of regulation on the flood-ring occurrence (visual intensity and relative frequency) and on ring widths in Fraxinus nigra trees growing at five sites distributed along the Driftwood River floodplain. Driftwood River was regulated by a dam in 1917 that was replaced at the same location in 1953. Ring width revealed little, to no evidence, of the impact of river regulation, in contrast to the flood rings. Prior to 1917, high relative frequencies of well-defined flood rings were recorded during known flood years, as indicated by significant correlations with reconstructed spring discharge of the nearby Harricana River. After the construction and the replacement of the dam, relative frequencies of flood rings and their intensities gradually decreased. Flood-ring relative frequencies after 1917, and particularly after 1953, were mostly composed of weakly defined (less distinct) flood rings with some corresponding to known flood years and others likely reflecting dam management. The strength of the correlations with the instrumental Harricana River discharge also gradually decrease starting after 1917. Compared with upper floodplain trees, shoreline trees at each site recorded flood rings less frequently following the construction of the first but especially of the second dam, indicating that water level regulation limited flooding in the floodplains. Compared with the downstream site to the dam, the upstream ones recorded significantly more flood rings in the postdam period, reemphasizing the importance of considering the position of the site along with the river continuum and site conditions in relation to flood exposure. The results demonstrated that sampling trees in multiple riparian stands and along with various hydrological contexts at a far distance of the dams could help disentangle the flooding signal from the dam management signal.
Silica Nanoparticles as a Probable Anti-Oomycete Compound Against Downy Mildew, and Yield and Quality Enhancer in Grapevines: Field Evaluation, Molecular, Physiological, Ultrastructural, and Toxicity Investigations
Authors: Rashad Younes M. , El-Sharkawy Hany H. A. , Belal Bassam E. A. , Abdel Razik Elsayed S. , Galilah Doaa A.
Published: October 2021

Downy mildew is the most destructive disease of grapevines in the regions of relatively warm and humid climate causing up to 50% yield losses. Application of silicon- (Si-) based products have been extensively studied against various oomycete, fungal, bacterial, and viral plant diseases, but studies on Si application in their nanosize are limited. In this study, the field application of silica nanoparticles (SiNPs) on Thompson Seedless grapevines (H4 strain) infected with downy mildew was evaluated. In addition, molecular, physiological, ultrastructural, and toxicity investigations were also conducted. The obtained results revealed that spraying of grapevines with SiNPs at 150 ppm significantly overexpressed the transcription factor jasmonate and ethylene-responsive factor 3 recording 8.7-fold, and the defense-related genes β-1,3-glucanase (11-fold), peroxidase (10.7-fold) pathogenesis-related-protein 1 (10.6-fold), and chitinase (6.5-fold). Moreover, a reduction up to 81.5% in the disease severity was achieved in response to this treatment. Shoot length and yield per grapevine were considerably enhanced recording up to 26.3 and 23.7% increase, respectively. The berries quality was also improved. Furthermore, this treatment led to an enhancement in the photosynthetic pigments, induction of phenolic and ascorbic acid contents, an increase in the activity of peroxidase and polyphenol oxidase enzymes, and a reduction in the cellular electrolyte leakage, lipid peroxidation, and H2O2 content. Scanning electron microscopy observations showed an increase up to 86.6% in the number of closed stomata and a reduction up to 55% in the average stomatal pore area in response to this treatment. Observations of the transmission electron microscopy showed ultrastructural alterations in the cells of a grapevine leaf due to the infection with downy mildew, including plasmolysis and disruption of the cellular components, abnormal chloroplasts, and thickening of the cell wall and cell membrane. These abnormal alterations were reduced in response to SiNPs spray. In contrast, this study also showed that this treatment had considerable cytotoxic and genotoxic effects at this direct dose/concentration. So, additional investigations to determine the SiNPs residue in the produced edible plant parts are urgently needed. In addition, the pre-harvest interval, toxicity index, and risk assessment should be evaluated before any recommendation for use.
It Is Just a Matter of Time: Balancing Homologous Recombination and Non-homologous End Joining at the rDNA Locus During Meiosis
Authors: Sims Jason , Rabanal Fernando A. , Elgert Christiane , von Haeseler Arndt , Schlögelhofer Peter
Published: October 2021

Ribosomal RNA genes (rDNAs) are located in large domains of hundreds of rDNA units organized in a head-to-tail manner. The proper and stable inheritance of rDNA clusters is of paramount importance for survival. Yet, these highly repetitive elements pose a potential risk to the genome since they can undergo non-allelic exchanges. Here, we review the current knowledge of the organization of the rDNA clusters in Arabidopsis thaliana and their stability during meiosis. Recent findings suggest that during meiosis, all rDNA loci are embedded within the nucleolus favoring non-homologous end joining (NHEJ) as a repair mechanism, while DNA repair via homologous recombination (HR) appears to be a rare event. We propose a model where (1) frequent meiotic NHEJ events generate abundant single nucleotide polymorphisms and insertions/deletions within the rDNA, resulting in a heterogeneous population of rDNA units and (2) rare HR events dynamically change rDNA unit numbers, only to be observed in large populations over many generations. Based on the latest efforts to delineate the entire rDNA sequence in A. thaliana, we discuss evidence supporting this model. The results compiled so far draw a surprising picture of rDNA sequence heterogeneity between individual units. Furthermore, rDNA cluster sizes have been recognized as relatively stable when observing less than 10 generations, yet emerged as major determinant of genome size variation between different A. thaliana ecotypes. The sequencing efforts also revealed that transcripts from the diverse rDNA units yield heterogenous ribosome populations with potential functional implications. These findings strongly motivate further research to understand the mechanisms that maintain the metastable state of rDNA loci.
The Role of Collectivism–Individualism in Attitudes Toward Compliance and Psychological Responses During the COVID-19 Pandemic
Authors: Xiao Wen S.
Published: October 2021

This study examined the role of individual differences in horizontal and vertical individualism and collectivism, trust and worries, and concerns about COVID-19 in predicting the attitudes toward compliance of health advice and psychological responses during the COVID-19 pandemic. Chinese university students (N=384, 324 female) completed measures of individualism and collectivism, trust, attitudes toward compliance, and psychological responses to the pandemic. Results showed that not only vertical collectivist orientation but also horizontal individualist orientation significantly predicted higher willingness to comply, whereas vertical individualist orientation significantly predicted lower willingness to comply. Vertical individualist and vertical collectivist orientations predicted higher psychological response in terms of distress, anxiety, and depression, while horizontal collectivistic orientation significantly predicted less psychological problems. Implications of the effect of individual-level cultural orientations on attitudes toward public health compliance and psychological well-being during global health crises are discussed.
An Integrated Mechanistic Model of Mindfulness-Oriented Recovery Enhancement for Opioid-Exposed Mother–Infant Dyads
Authors: Reese Sarah E. , Conradt Elisabeth , Riquino Michael R. , Garland Eric L.
Published: October 2021

A growing body of neurobiological and psychological research sheds light on the mechanisms underlying the development and maintenance of opioid use disorder and its relation to parenting behavior. Perinatal opioid use is associated with risks for women and children, including increased risk of child maltreatment. Drawing from extant data, here we provide an integrated mechanistic model of perinatal opioid use, parenting behavior, infant attachment, and child well-being to inform the development and adaptation of behavioral interventions for opioid-exposed mother–infant dyads. The model posits that recurrent perinatal opioid use may lead to increased stress sensitivity and reward dysregulation for some mothers, resulting in decreased perceived salience of infant cues, disengaged parenting behavior, disrupted infant attachment, and decreased child well-being. We conclude with a discussion of Mindfulness-Oriented Recovery Enhancement as a means of addressing mechanisms undergirding perinatal opioid use, parenting, and attachment, presenting evidence on the efficacy and therapeutic mechanisms of mindfulness. As perinatal opioid use increases in the United States, empirically informed models can be used to guide treatment development research and address this growing concern.
The “100,000+ Clicks” Dream Comes True: A Study on the Click-and-Read Behavior of WeChat Tweets From the Perspective of Emotional Expression
Authors: Fan Jun , Chen Tingting , Lin Li
Published: October 2021

An in-depth and refined empirical study on the emotional expression of information and the information processing mechanism of audiences is carried out to provide enterprises and other organizations with insights and references as regard to the effective utilization of WeChat Tweets for information dissemination and marketing purposes. Based on 1,465 actual tweets from two different types of WeChat public accounts (knowledge communication and information releasing), this paper applies the limited attention capacity model and the signaling theory to analyze the influence of emotional presence, emotional complexity, emotional intensity, and emotional polarity of tweet titles on the click-and-read behavior of the audience. The results show that for WeChat public accounts serving the purpose of knowledge communication, emotional presence and emotional complexity of tweet titles, as well as the emotional intensity of positive tweet titles, has no significant effect on the click-and-read behaviors of the audience. Besides, the emotional intensity of negative tweet titles has a significant negative impact on the audience’s click-and-read behaviors. While for WeChat public accounts serving the purpose of information releasing, tweet titles with emotional presence and lower level of emotional complexity are more likely to trigger click-and-read behaviors of audiences; emotional intensity of negative tweet titles has no significant effect on the click-and-read behaviors of audiences, and emotional intensity of positive tweet titles has a significant negative impact on the audience’s click-and-read behaviors. Thus, this study further analyzes the influence of emotional factors, such as emotional existence, emotional complexity, emotional intensity, and emotional polarity of tweet titles on the click-and-read behavior of consumers and further explores the emotional information processing mechanism of WeChat tweet readers.
Adult Aging Moderates the Relationship Between Trait Cognitive Anxiety and Subjective Everyday Cognitive Difficulties
Authors: Spalding David M. , MacAngus Kerry , Moen Martine K. , Nicholls Louise A. Brown
Published: October 2021

The present aim was to determine, across the adult lifespan, the extent to which different dimensions of trait anxiety might affect subjective cognitive difficulties in everyday life. Following Attentional Control Theory (ACT; Eysenck et al., 2007), we predicted that trait anxiety would have a greater effect on attention and verbal abilities than on visual abilities. We also expected trait cognitive anxiety to exhibit more robust relationships with cognition than trait somatic anxiety. Importantly, we predicted that effects of anxiety would be greater in older adults, in line with the Strength and Vulnerability Integration model (SAVI; Charles, 2010). The sample comprised 286 United Kingdom-based adults aged 18–93 years. Participants completed self-report measures of trait cognitive and somatic anxiety (the State-Trait Inventory for Cognitive and Somatic Anxiety; STICSA, Ree et al., 2008) and everyday cognitive difficulties (the Multiple Abilities Self-Report Questionnaire; MASQ, Seidenberg et al., 1994). Moderated regression models were constructed, including trait cognitive or somatic anxiety as a predictor of cognitive difficulties, and age as the moderator variable. Covariates included depression, stress (the Depression Anxiety Stress Scales—short form; DASS-21, Lovibond and Lovibond, 1995), gender, current mental health treatment status, and physical health status. When cognitive anxiety was the predictor variable, somatic anxiety was also included as a covariate, and vice-versa. Trait cognitive anxiety and age interacted to predict all MASQ subscales other than visual-perceptual ability. Difficulties with attention, verbal memory, and language abilities were significantly greater at higher levels of anxiety for all age groups, with the effect greatest in older adults. Difficulties with visual-spatial memory were significantly greater at higher levels of anxiety in middle-aged and older adults only. Higher trait somatic anxiety predicted difficulties with verbal memory and language ability independently of age, and interacted with age to predict language difficulties. Interestingly, age also significantly predicted less subjective difficulty with attention, independently of anxiety level. The results show that trait cognitive and somatic anxiety are both related to subjective, everyday cognitive difficulties. However, effects of trait cognitive anxiety are more robust across cognitive domains and tend to increase, or first appear, over the course of the adult lifespan.
Doctor-Patient Relationship in the Eyes of Medical Professionals in China During the COVID-19 Pandemic: A Cross-Sectional Study
Authors: Zhou Yanan , Yang Winson Fu Zun , Ma Yuejiao , Wu Qiuxia , Yang Dong , Liu Tieqiao , Wu Xiaoming
Published: October 2021

Background: Doctor-patient relationship (DPR) is very important for patient outcomes, especially during a public health emergency like the COVID-19 pandemic. However, few studies have evaluated DPR and related sentiments from medical professionals' perspectives. Thus, the aim of the study is to provide a better understanding of DPR from medical professionals' perspectives during the COVID-19 pandemic in China.

Methods: A total of 979 medical professionals, including doctors, nurses, technicians, and other workers have completed a series of questionnaires to evaluate their attitudes toward DPR, trust, violence against doctors, factors that affected and improved DPR, and the importance of these factors on DPR. Analyses of variances (ANOVA) and linear regressions were used to analyze the effects of the pandemic, demographic variables, and various elements on DPR.

Results: One-way ANOVA revealed a significant effect of education on recent DPR [F(2, 976) = 6.17, p < 0.001 and trust at F(2, 976) = 9.54, p < 0.001], indicating that individuals with higher level of education (bachelor's degree, Master's degree and above) showed poorer recent DPR and lower level of trust. The level of hospital also showed a significant effect on trust [F(5, 973) = 3.79, p = 0.0021]. Cochran's Q test revealed a significant difference in factors that affected [Q(11) = 3,997.83, p < 0.001] and improved [Q(8) = 3,304.53, p < 0.001] DPR. Backward stepwise linear regressions revealed predictors for changes during [F(9, 969) = 21.17, p < 0.001, R2 = 0.16], shortly after [F(7, 971) = 54.98, p < 0.001, R2 = 0.28], and long after [F(10, 968) = 37.83, p < 0.001, R2 = 0.29] the pandemic.

Conclusions: Medical professionals' perceptions of DPR is important as they provide basis for the improvement in working environment of medical professionals and hospital visiting experience of patients, as well as healthcare policy making and preparation for future public health emergencies.
Factors Affecting Perceived Health Benefits and Use Behaviors in Urban Green Spaces During the COVID-19 Pandemic in Southern China Megacities
Authors: Li Haiwei , Luo Weijing , Hou Yongqi , Xia Yu , Yao Jing , Kang Ning , Deng Congshuang , Sun Hu , Chen Chongxian
Published: October 2021

Background: The COVID-19 pandemic has alienated people from urban green spaces (UGSs) that have various health outcomes for humans. However, little is known about the influential factors of perceived health benefits and use behaviors in UGSs during the COVID-19 pandemic. This study aims to explore the key factors that influence perceived health benefits and use behaviors in UGSs and to assess the mediating role of place attachment in relationships during the COVID-19 pandemic in Chinese megacities.

Methods: We conducted an online questionnaire survey from December 2020 to March 2021 in Guangzhou and Shenzhen, China. Six multiple regression models were constructed to investigate the main factors by which UGSs influence citizens' perceived health benefits and use behaviors. Four mediation models were established using the structural equation modeling (SEM) method to explore the mediating effect of place attachment.

Results: A total of 628 questionnaires were included in the analysis. The results revealed that some UGS components (green space access, maintenance, and soundscape) significantly affected perceived health benefits for citizens (physical, mental, and social health) during the COVID-19 pandemic. Conversely, use behaviors (frequency of visits, duration of visits, and activity intensity) were mainly affected by the sociodemographic context but less affected by UGS components. In addition, UGS components were found to significantly predict place attachment, which in turn influenced the perceived health benefits, frequency, and duration of visits.

Conclusions: This study distinguished the key factors that affect perceived health benefits and use behaviors during the COVID-19 pandemic: green space access, maintenance, soundscape, and sociodemographic characteristics. Place attachment still needs to be considered when discussing how to encourage citizens to visit UGSs during the pandemic. These findings provide implications for policymakers and landscape planners regarding design and management measures for UGSs that are conducive to coping with pandemics.
Postoperative Pain After Robot-Assisted Laparoscopic Ventral Hernia Repair
Authors: Lindström Per , Rietz Göran , Everhov Åsa Hallqvist , Sandblom Gabriel
Published: October 2021

Background: Robot-assisted ventral hernia repair, when performed correctly, may reduce the risk for pain and discomfort in the postoperative period thus enabling shorter hospital stay. The aim of the present study was to evaluate postoperative pain following robot-assisted laparoscopic repair. The approach was selected after an intraoperative decision to complete the repair as: (1). Transabdominal Preperitoneal Repair (TAPP); (2). Trans-Abdominal RetroMuscular (TARM) repair; or (3). Intraperitoneal Onlay Mesh (IPOM) repair depending on anatomical conditions.

Methods: Twenty ventral hernia repairs, 8 primary and 12 incisional, were included between 18th Dec 2017 and 11th Nov 2019. There were 8 women, mean age was 60.3 years, and mean diameter of the defect was 3.8 cm. The repairs were performed at Södersjukhuset (Southern General Hospital, Stockholm) using the Da Vinci Si Surgical System®. Sixteen repairs were completed with the TAPP technique, 2 with the TARM technique, and 2 as IPOM repair.

Results: Mean hospital stay was 1.05 days. No postoperative infection was seen, and no recurrence was seen at 1 year. At the 30-day follow-up, fifteen patients (75%) rated their pain as zero or pain that was easily ignored, according to the Ventral Hernia Pain Questionnaire. After 1 year no one had pain that was not easily ignored.

Conclusion: The present study shows that robot-assisted laparoscopic ventral hernia is feasible and safe. More randomized controlled trials are needed to show that the potential benefits in terms of shorter operation times, earlier discharge, and less postoperative pain motivate the extra costs associated with the robot technique.
Predictive Analytics of Cattle Host and Environmental and Micro-Climate Factors for Tick Distribution and Abundance at the Livestock–Wildlife Interface in the Lower Okavango Delta of Botswana
Authors: Babayani Nlingisisi D. , Makati Anastacia
Published: October 2021

Tick distribution and abundance is influenced by several factors that include micro-climate and environmental and host factors. Contextual understanding of the role played by these factors is critical to guide control measures. The aim of this study was to determine the predictive value of these factors for changes in tick distribution and abundance at the livestock–wildlife interface of the lower Okavango Delta. A two-stage quota sampling design was adopted to select 30 clusters of seven cattle each for tick investigation. Tick investigation was done by lifting the tail to count the total number of ticks at the anno-vulva region during the four meteorological seasons of the year. Additional data were collected on ear tag number, location of origin, sex, age, body condition score (BCS), season of the year, stocking density, and Normalized Difference Vegetation Index values of source terrain. A random effects model was used to evaluate predictive value of the above for tick abundance. Additional mapping of tick distribution pattern in the abattoir catchment area was conducted using spatial autocorrelation and hot-spot analysis. Tick intensity of infection increased linearly from males to females (Z = 3.84, p < 0.001), decreased linearly from lower to higher BCS (Z = −4.11, p < 0.001), and increased linearly from cold-dry through dry to wet seasons (Z = 10.19, p < 0.001). Significant clustering of neighboring crushes on account of tick count was noted in the late-hot-dry season, with high tick count in crushes located along the seasonal flood plains and low tick counts in those located in the dry grasslands. It was concluded from this study that cattle tick abundance is influenced largely by season of the year and that the micro-climatic conditions brought about by the seasonal flooding of the delta have a decided effect on tick distribution during the driest of the seasons. Our study has, for the first time, profiled drivers of tick distribution and population growth in this unique ecosystem. This has the potential to benefit human and veterinary public health in the area through implementation of sustainable tick control strategies that are not heavily reliant on acaricides.
Acidification Effects on In Situ Ammonia Emissions and Cereal Yields Depending on Slurry Type and Application Method
Authors: Wagner Christian , Nyord Tavs , Vestergaard Annette Vibeke , Hafner Sasha Daniel , Pacholski Andreas Siegfried
Published: October 2021

Field application of organic slurries contributes considerably to emissions of ammonia (NH3) which causes sever environmental damage and can result in lower nitrogen (N) fertilizer efficiency. In recent years, field acidification systems have been introduced to reduce such NH3 emissions. However, combined field data on ammonia emissions and N use efficiency of acidified slurries, in particular by practical acidification systems, are scarce. Here, we present for the first time a simultaneous in situ assessment of the effects of acidification of five different organic slurries with a commercial acidifications system combined with different application techniques. The analysis was performed in randomized plot trials in winter wheat and spring barley after two applications to each crop (before tillering and after flag leave emergence) in year 2014 in Denmark. Slurry types included cattle slurry, mink slurry, pig slurry, anaerobic digestate, and the liquid phase of anaerobic digestate. Tested application techniques were trail hose application with and without slurry acidification in winter wheat and slurry injection and incorporation compared to trail hose application with and without acidification in spring barley. Slurries were applied on 9 m × 9 m plots separated by buffer areas of the same dimension. Ammonia emission was determined by a combination of semi-quantitative acid traps scaled by absolute emissions obtained from Draeger Tube Method dynamic chamber measurements. Experimental results were analysed by mixed effects models and HSD post hoc test (p < 0.05). Significant and almost quantitative NH3 emission reduction compared to trail hose application was observed in the barley trial by slurry incorporation of acidified slurry (89% reduction) and closed slot injection (96% reduction), while incorporation alone decreased emissions by 60%. In the two applications to winter wheat, compared to trail hose application of non-acidified slurry, acidification reduced NH3 emissions by 61% and 67% in cattle slurry, in anaerobic digestate by 45% and 57% and liquid phase of anaerobic digestate by 58%, respectively. Similar effects but on a lower emission level were observed in mink slurry, while acidification showed almost no effect in pig slurry. Acidifying animal manure with a commercial system was confirmed to consistently reduce NH3 emissions of most slurry types, and emission reductions were similar as from experimental acidification systems. However, failure to reduce ammonia emissions in pig slurry hint to technical limitations of such systems. Winter wheat and spring barley yields were only partly significantly increased by use of ammonia emission mitigation measures, while there were significant positive effects on apparent nitrogen use efficiency (+17–28%). The assessment of the agronomic effects of acidification requires further investigations.