bluuebunny/crossref_metadata_2025 huggingface dataset:
Changes in a Comprehensive Profile of Saliva Analytes in Fattening Pigs during a Complete Productive Cycle: A Longitudinal Study
Authors: Ortín-Bustillo Alba , Escribano Damián , López-Arjona Marina , Botia María , Fuentes Pablo , Martínez-Miró Silvia , Rubio Camila P. , García-Manzanilla Edgar , Franco-Martínez Lorena , Pardo-Marín Luis , Cerón José J. , Llonch Pol , Tecles Fernando
Published: July 2022

A comprehensive panel of 29 salivary analytes was measured in fattening pigs to evaluate its possible changes along their productive cycle. The identification of those changes would allow a better interpretation of the results according to the productive phase of the animal. Saliva samples were obtained from 49 Large-White pigs (24 females, 25 males) in suckling phase, at the beginning and the end of the nursery phase, and at the beginning and the end of the growing phase. Several analytes changed according to the phase of the productive cycle, with most of the analytes showing higher values at lactation and at the beginning of nursery. Additionally, differences were seen due to sex. When possible relations between performance parameters and analytes were evaluated, significant positive but weak relationships were found between weight at birth and salivary γ-glutamyl transferase, and between back-fat thickness and salivary lactate dehydrogenase. In conclusion, differences in the values of salivary analytes can be found in fattening pigs depending on the productive phase and sex of the animals.
Focus on Nitric Oxide Homeostasis: Direct and Indirect Enzymatic Regulation of Protein Denitrosation Reactions in Plants
Authors: Treffon Patrick , Vierling Elizabeth
Published: July 2022

Protein cysteines (Cys) undergo a multitude of different reactive oxygen species (ROS), reactive sulfur species (RSS), and/or reactive nitrogen species (RNS)-derived modifications. S-nitrosation (also referred to as nitrosylation), the addition of a nitric oxide (NO) group to reactive Cys thiols, can alter protein stability and activity and can result in changes of protein subcellular localization. Although it is clear that this nitrosative posttranslational modification (PTM) regulates multiple signal transduction pathways in plants, the enzymatic systems that catalyze the reverse S-denitrosation reaction are poorly understood. This review provides an overview of the biochemistry and regulation of nitro-oxidative modifications of protein Cys residues with a focus on NO production and S-nitrosation. In addition, the importance and recent advances in defining enzymatic systems proposed to be involved in regulating S-denitrosation are addressed, specifically cytosolic thioredoxins (TRX) and the newly identified aldo-keto reductases (AKR).
Correlation between Scoliosis Flexibility Degree on Preoperative Imaging with Postoperative Curve Correction and Mechanical Complications
Authors: Tavares Junior Mauro Costa Morais , Marcon Raphael Martus , Cristante Alexandre Fogaça , Letaif Olavo Biraghi
Published: July 2022

(1) Background: In the preoperative planning stage of scoliosis surgery, it is routine to use radiographs obtained with and without traction to observe the curve flexibility in order to estimate curve correction, but its association with mechanical complications is not completely understood. (2) Methods: Retrospective cohort study of all patients undergoing infantile, congenital, neuromuscular or idiopathic adolescent scoliosis correction surgery at a single institution between 2015 and 2019, with a minimum follow-up of 24 months. Associations between qualitative variables were tested with the chi-square test. The association between qualitative and quantitative variables were tested with the Mann–Whitney test, and correlations between quantitative variables was tested with Spearman’s correlation. (3) Results: A total of 330 patients, 88 males and 242 females, with a mean age of 16.98 years at surgery, were included. The mean value of preoperative main curves, its flexibility and postoperative value were 54.44 degrees, 21.73 degrees and 18.08 degrees, respectively. (4) Conclusions: Preoperative spinal X-ray examination with traction or bending films is a reasonable option for assessing scoliotic curve flexibility, and patients with neuromuscular scoliosis who are not ambulatory can be informed of the increased risks of late mechanical complications.
Identification by GC-MS Analysis of Organics in Manufactured Articles through a D-Optimal Design
Authors: Romagnoli Monica , Polliotto Valeria , Alladio Eugenio , Pazzi Marco
Published: July 2022

Many manufactured articles are made of composite materials often bonded by a phenolic resin. Through a D-optimal design, we optimized a method to characterize phenolic resins after the extraction process by GC-MS analysis. The study was conducted on three different phenolic resins and four manufactured articles with the same inorganic composition and different analyzed binders. Moreover, three cardanol resins that differ in their production systems were analyzed to see if there were differences between them. Through Soxhlet extraction with dichloromethane or acetone, it is possible to differentiate the raw materials through characteristic compounds and to identify them in the manufactured articles.
Effects of Caffeine and 5-Caffeoylquinic Acid on Blood Cell In Vitro Cytokine Production in Response to Lipopolysaccharide Stimulation
Authors: Rodas Lluis , Martínez Sonia , Riera-Sampol Aina , Moir Hannah J. , Tauler Pedro
Published: July 2022

Previous in vitro studies the have suggested anti-inflammatory properties of caffeine. Coffee is also rich in phenolic acids with several potential health benefits (chlorogenic acids), mainly 5-caffeoylquinic acid (5-CQA). The aim of the present study was to determine the effect of caffeine and 5-CQA, using concentrations comparable to typical human consumption, on LPS-induced cytokine production in whole blood in vitro cultures. Whole blood samples (n = 10) were stimulated for 24 h with LPS in the presence of caffeine or 5-CQA. The concentrations of various cytokines, malondialdehyde (MDA) and adenosine-3′,5′-cyclic monophosphate (cAMP) were determined in the culture media. Significant effects of caffeine were found only for the stimulated production of TNF-α (p = 0.014) and cAMP (p = 0.001). However, only the highest caffeine concentration (50 μM) induced lower TNF-α (p = 0.016) and higher cAMP (p = 0.006) levels in comparison to the control. The 5-CQA only influenced the stimulated production of IL-6 (p = 0.002), with lower values for the highest 5-CQA concentrations (2 μM: p = 0.012; 20 μM: p < 0.001). Neither caffeine (p = 0.227) nor 5-CQA (p = 0.259) influenced MDA concentrations. In conclusion, both caffeine and 5-CQA exerted limited in vitro anti-inflammatory effects. For caffeine, these effects were attained with high physiological concentrations. However, for 5-CQA, supraphysiological concentrations were needed.
Random Vibration Fatigue Life Analysis of Airborne Electrical Control Box
Authors: Zhang Daqian , Chen Yueyang
Published: July 2022

To study the influence of random vibration on the fatigue life of airborne equipment, an aircraft electrical control box was selected as the research object. First, finite element software was used to model the dynamics of the airborne electrical control box to investigate its mode frequencies. The accuracy of finite element simulations was verified by performing mode experiments. Second, the mode superposition method was used to analyze the flight direction (X axis), side direction (Y axis), and altitude direction (Z axis) random vibration responses of the electrical control box. The analysis results were combined with the Miner linear cumulative damage criterion and the Gaussian-distribution Steinberg method to estimate the fatigue life of the electrical control box in the three directions. Finally, the calculation results were verified by performing the random vibration durability test on the electrical control box. The finite element mode analysis results show good agreement with the vibration experiment results, and the maximum error is 13.4%, indicating that the finite element model established in this paper is acceptable. The fatigue life of the electrical control box in the three axes meets the user requirements, and random vibration along the side direction (Y axis) has the greatest impact on the fatigue life, which is consistent with the results of the actual experimental data. The research method can be extended to predict the fatigue life of other airborne equipment and thus has practical significance for structural design and reliability analysis of airborne equipment.
Stability of a Nonlinear ML-Nonsingular Kernel Fractional Langevin System with Distributed Lags and Integral Control
Authors: Zhao Kaihong
Published: July 2022

The fractional Langevin equation has more advantages than its classical equation in representing the random motion of Brownian particles in complex viscoelastic fluid. The Mittag–Leffler (ML) fractional equation without singularity is more accurate and effective than Riemann–Caputo (RC) and Riemann–Liouville (RL) fractional equation in portraying Brownian motion. This paper focuses on a nonlinear ML-fractional Langevin system with distributed lag and integral control. Employing the fixed-point theorem of generalised metric space established by Diaz and Margolis, we built the Hyers–Ulam–Rassias (HUR) stability along with Hyers–Ulam (HU) stability of this ML-fractional Langevin system. Applying our main results and MATLAB software, we have carried out theoretical analysis and numerical simulation on an example. By comparing with the numerical simulation of the corresponding classical Langevin system, it can be seen that the ML-fractional Langevin system can better reflect the stationarity of random particles in the statistical sense.
Conventional and Resin-Modified Glass Ionomer Cement Surface Characteristics after Acidic Challenges
Authors: Nica Irina , Stoleriu Simona , Iovan Alexandru , Tărăboanță Ionuț , Pancu Galina , Tofan Nicoleta , Brânzan Răzvan , Andrian Sorin
Published: July 2022

The aim of the present study was to assess by atomic force microscopy (AFM) the surface roughness of a traditional glass ionomer cement- GIC (Fuji IX GP, GC Corporation, Tokyo, Japan) and a resin modified glass ionomer cement- RMGIC (Vitremer, 3M ESPE, St. Paul, MN, USA) after different immersion regimes on some acidic drinks. Sixteen cylindrical samples having the height of 5 mm and the thickness of 2 mm were obtained from each material and they were divided into two groups: Group I (Fuji IX samples) and Group II (Vitremer samples). Specimens of each group were then randomly divided into 4 subgroups: subgroup A (control)—15 samples were kept in artificial saliva and in the other three subgroups, each having 15 samples the samples were immersed in Coca-Cola (subgroup B), Cappy lemonade and mint (subgroup C) and Fuzetea (subgroup D) for 7 days (subgroups A1–D1), 14 days (subgroups A2–D2), and 21 days (subgroups A3–D3). AFM qualitative and quantitative surface evaluation (mean value of surface roughness parameter, Sa) of each sample was performed. The highest surface roughness was determined when both materials were submerged 14 days in acidic drinks. Traditional GIC was more affected by acidic environment when comparing to RMGIC.
The Interplay of Biomimetics and Biomechatronics
Authors: Witte Hartmut
Published: July 2022

Biomechatronics is an engineering subject in which biomimetics as a method is one of its two supporting pillars: biology for engineering, or Bio4Eng. This is contrasted with biocompatible design, or Eng4Bio, examples of which are human-serving systems, such as exoskeletons, and biomedical engineering. The paper aims to illustrate that the research fields of biomimetics, biomechatronics, and biomedical engineering are not in competition but mutually supportive. The current attempts to place biomechatronics under the umbrella of biomimetics or biomedical engineering are therefore not expedient; they deprive the subject of its strength of combining Bio4Eng and Eng4Bio at any time in a task-related manner. In addition to research and development, however, the training of the specialists supporting the subjects must not be disregarded and is therefore described based on a proven design.
Red Blood Cell Sedimentation Index Using Shear Stress of Blood Flow in Microfluidic Channel
Authors: Kang Yang Jun
Published: July 2022

Red blood cell sedimentation has been used as a promising indicator of hematological diseases and disorders. However, to address several issues (i.e., syringe installation direction, blood on-off flow control, image-based quantification, and hemodilution) raised by the previous methods, it is necessary to devise a new method for the effective quantification of red blood cell sedimentation under a constant blood flow. In this study, the shear stress of a blood flow is estimated by analyzing an interface in a co-flowing channel to quantify the red blood cell sedimentation in blood syringes filled with blood (hematocrit = 50%). A red blood cell sedimentation index is newly suggested by analyzing the temporal variations in the shear stress. According to the experimental investigation, the sedimentation index tends to decrease at a higher flow rate. A higher level of hematocrit has a negative influence on the sedimentation index. As a performance demonstration of the present method, the red blood cell sedimentation processes of various test bloods were quantitatively compared in terms of the shear stress, image intensity, and sedimentation velocity. It was found that the proposed index provided a more than 10-fold increase in sensitivity over the previous method (i.e., image intensity). Additionally, it provided more consistent results than another conventional sedimentation method (sedimentation velocity). In conclusion, the present index can be effectively adopted to monitor the red blood cell sedimentation in a 10-min blood delivery.
A Rasch Analysis of Students’ Academic Motivation toward Mathematics in an Adaptive Learning System
Authors: Lim Lyndon , Lim Seo Hong , Lim Wei Ying Rebekah
Published: July 2022

Despite the criticality of considering student academic motivation as it influences learning, research within the field of adaptive learning technologies for education has so far focused more on customising instruction to implement personalised learning, than investigating how personalised learning is associated with learners’ motivation. Given this, a robust instrument is required to gather information about student academic motivation within the context of adaptive learning technologies. This study sought to validate the Academic Motivation Toward Mathematics Survey (AMTMS) currently used to measure motivation based on self-determination theory in mathematics education at pre-tertiary levels (grades 11 and 12) in Asia. A total of 196 participants recruited via availability sampling took part in this study, after interacting with an in-house mathematics adaptive learning system within a tertiary educational institution. The validation was performed based on modern test theory given that it overcomes some limitations of classical factor analytic approaches. Results supported the factorial structure of the AMTMS but 12 of the original 21 items had to be rescored to establish ordered thresholds. Further, the bifactor equivalent solution suggested the possibility of reporting a singular motivation index comprising the five factors within the AMTMS. Along with the results, this study offers researchers a robust and validated instrument to measure motivation toward mathematics that can be used within an adaptive learning environment.
Morphological Evolution of Passive Soil Arch in Front of Horizontal Piles in Three Dimensions
Authors: Ren Xiang , Luo Lijuan , Zheng Yunxin , Ma Jiakuan , An Xuexu
Published: July 2022

The anti-slide pile is a primary method of landslide control. The effect of the passive soil arch in front of the embedded section of piles has a significant effect on the anti-slide pile’s bearing capacity. The upgraded model test scheme was used to conduct model tests with a pile spacing four times the width of the pile and a geometric scale ratio of 1:15. The anti-slide pile stress, pile bending strain, and soil stress in front of the pile were all studied in relation to the loading amount. In addition to the model test, the numerical simulation method was utilized to investigate the three-dimensional morphological change of the passive soil arch in front of the pile. The results indicated that: clearly, the side piles can eliminate the border effect. The distribution of pile bending strain along the pile after loading is referred to as a parabola. Bending failure occurred at a depth of 40 mm, approximately 0.9 m from the pile top. Under the condition that the pile spacing is four times the pile width, a passive soil arch occurs in front of the anti-slide pile’s fixed part, and its development can be split into four stages: formation, development, completion, and destruction. The passive soil arches in front of the piles are generated and destroyed gradually along the buried depth, and the three-dimensional surface of the space drops gradually along the buried depth with the loading amount and advances toward the loading direction until the anti-slide pile system fails. The research findings and experiences can serve as a basis for future research.
Governance, Standards and Regulation: What Construction and Mining Need to Commit to Industry 4.0
Authors: Smith Kai , Sepasgozar Samad
Published: July 2022

Digital transformation has become a pressing concern for the Australian government in the wake of COVID-19. While a thriving construction industry is key to Australia’s economic recovery, the promised land of Industry 4.0 continues to elude the sector. Unlike the mining industry, which has obtained government funding to future-proof its workforce, the building industry remains at risk of being left behind because it has failed to prosecute the case for its own planned Fourth Industrial Revolution. A consistent approach to both sectors is needed to mitigate against asymmetries in the workforce and assist those transitioning from sectors devastated by COVID-19 by providing them with the high-tech skills which fortify the link between wages and employment. SMEs given their limited resources are also vulnerable, and the sector has been rocked by waves of insolvencies in recent times. Achieving Industry 4.0 success has long been a goal among industry academics yet hardly any attention is paid to the institution or its failures. This study subjected 59 authoritative articles to bibliometric analysis and systematic literature review and identified a dearth of research on how best to regulate Industry 4.0 and deliver the standards on which construction and mining businesses will depend when making the choice to commit to Industry 4.0. Nevertheless, there are valuable lessons to be learnt when it comes to supporting SMEs and workers embarking the risky business of Digital Transformation.
Overexpression of NNMT in Glioma Aggravates Tumor Cell Progression: An Emerging Therapeutic Target
Authors: Sun Wei , Zou Yongxiang , Cai Zheng , Huang Jinxiang , Hong Xinjie , Liang Qiang , Jin Weilin
Published: July 2022

Purpose: Increasing evidence has revealed that nicotinamide N-methyltransferase (NNMT) is a key factor influencing the prognosis of tumors. The present study aimed to investigate the role of NNMT in glioma and to elucidate the associated functional mechanisms. Methods: Clinical samples were analyzed by immunohistochemical staining and Western blotting to evaluate NNMT expression in glioma and normal brain tissues. The correlation between NNMT expression and glioma was analyzed using the Cancer Genome Atlas (TCGA) database. Additionally, NNMT was knocked down in two types of glioma cells, U87 and U251, to evaluate the invasive ability of these cells. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to validate NNMT knockdown in the cells. Furthermore, ELISA was used to determine the balance between nicotinamide adenine dinucleotide and nicotinamide adenine dinucleotide hydrogen (NAD/NADH ratio), which verified the altered methylation patterns in the cells. The glioma xenograft mouse models were used to verify the regulatory role of NNMT, GAP43, and SIRT1. Results: Analysis based on our clinical glioma samples and TCGA database revealed that overexpression of NNMT was associated with poor prognosis of patients. Knockdown of NNMT reduced the invasive ability of glioma cells, and downregulation of its downstream protein GAP43 occurred due to altered cellular methylation caused by NNMT overexpression. Gene Set Enrichment Analysis confirmed that NNMT modulated the NAD-related signaling pathway and showed a negative association between NNMT and SIRT1. Moreover, the regulatory roles of NNMT, GAP43, and SIRT1 were confirmed in glioma xenograft mouse models. Conclusion: Overexpression of NNMT causes abnormal DNA methylation through regulation of the NAD/NADH ratio, which in turn leads to the downregulation of GAP43 and SIRT1, eventually altering the biological behavior of tumor cells.
Oleocanthal Attenuates Metastatic Castration-Resistant Prostate Cancer Progression and Recurrence by Targeting SMYD2
Authors: Siddique Abu Bakar , Ebrahim Hassan Y. , Tajmim Afsana , King Judy Ann , Abdelwahed Khaldoun S. , Abd Elmageed Zakaria Y. , El Sayed Khalid A.
Published: July 2022

Metastatic castration-resistant prostate cancer (mCRPC) is the most aggressive prostate cancer (PC) phenotype. Cellular lysine methylation is driven by protein lysine methyltransferases (PKMTs), such as those in the SET- and MYND-containing protein (SMYD) family, including SMYD2 methylate, and several histone and non-histone proteins. SMYD2 is dysregulated in metastatic PC patients with high Gleason score and shorter survival. The Mediterranean, extra-virgin-olive-oil-rich diet ingredient S-(-)-oleocanthal (OC) inhibited SMYD2 in biochemical assays and suppressed viability, migration, invasion, and colony formation of PC-3, CWR-R1ca, PC-3M, and DU-145 PC cell lines with IC50 range from high nM to low µM. OC’s in vitro antiproliferative effect was comparable to standard anti-PC chemotherapies or hormone therapies. A daily, oral 10 mg/kg dose of OC for 11 days effectively suppressed the progression of the mCRPC CWR-R1ca cells engrafted into male nude mice. Daily, oral OC treatment for 30 days suppressed tumor locoregional and distant recurrences after the primary tumors’ surgical excision. Collected OC-treated animal tumors showed marked SMYD2 reduction. OC-treated mice showed significant serum PSA reduction. For the first time, this study showed SMYD2 as novel molecular target in mCRPC, and OC emerged as a specific SMYD2 lead inhibitor. OC prevailed over previously reported SMYD2 inhibitors, with validated in vivo potency and high safety profile, and, therefore, is proposed as a novel nutraceutical for mCRPC progression and recurrence control.
A Face-To-Face Comparison of Tumor Chicken Chorioallantoic Membrane (TCAM) In Ovo with Murine Models for Early Evaluation of Cancer Therapy and Early Drug Toxicity
Authors: Rupp Tristan , Legrand Christophe , Hunault Marion , Genest Laurie , Babin David , Froget Guillaume , Castagné Vincent
Published: July 2022

Ethical considerations, cost, and time constraints have highlighted the need to develop alternatives to rodent in vivo models for evaluating drug candidates for cancer. The tumor chicken chorioallantoic membrane (TCAM) model provides an affordable and fast assay that permits direct visualization of tumor progression. Tumors from multiple species including rodents and human cell lines can be engrafted. In this study, we engrafted several tumor models onto the CAM and demonstrated that the TCAM model is an alternative to mouse models for preliminary cancer drug efficacy testing and toxicity analysis. Tumor cells were deposited onto CAM, and then grown for up to an additional 10 days before chronic treatments were administered. The drug response of anticancer therapies was screened in 12 tumor cell lines including glioblastoma, melanoma, breast, prostate, colorectal, liver, and lung cancer. Tumor-bearing eggs and tumor-bearing mice had a similar chemotherapy response (cisplatin and temozolomide) in four human and mouse tumor models. We also demonstrated that lethality observed in chicken embryos following chemotherapies such as cisplatin and cyclophosphamide were associated with corresponding side-effects in mice with body weight loss. According to our work, TCAM represents a relevant alternative model to mice in early preclinical oncology screening, providing insights for both the efficacy and the toxicity of anticancer drugs.
Clinical Usefulness of Monitoring Muscle Volume during Atezolizumab Plus Bevacizumab Therapy in Patients with Unresectable Hepatocellular Carcinoma
Authors: Matsumoto Hiroaki , Tsuchiya Kaoru , Nakanishi Hiroyuki , Hayakawa Yuka , Yasui Yutaka , Uchihara Naoki , Suzuki Keito , Tanaka Yuki , Miyamoto Haruka , Ishido Shun , Yamada Michiko , Keitoku Taisei , Nobusawa Tsubasa , Higuchi Mayu , Takaura Kenta , Tanaka Shohei , Maeyashiki Chiaki , Tamaki Nobuharu , Takahashi Yuka , Kurosaki Masayuki , Asahina Yasuhiro , Okamoto Ryuichi , Izumi Namiki
Published: July 2022

Background: Sarcopenia is associated with overall survival in patients with hepatocellular carcinoma (HCC). However, it is not known whether muscle volume is associated with clinical outcomes during combination therapy with immune checkpoint inhibitors. We investigated the relationship between changes in muscle volume and treatment outcomes in patients treated with atezolizumab plus bevacizumab. Methods: Thirty-two patients with HCC who received atezolizumab plus bevacizumab as the first-line treatment between October 2020 and February 2022 were included. Skeletal muscle mass index (SMI) was calculated from the skeletal muscle area at the L3 level of the lumbar vertebrae. We compared pretreatment SMI and SMI at 6–14 weeks after administration. Results: Of the 32 patients, 18 had a decreased SMI, while 14 did not. Progression-free survival (PFS) was significantly longer in patients without SMI decrease than in patients with SMI decrease (8.5 vs. 5.8 months, p = 0.011). There were no significant differences in treatment-related adverse events between the patients with and without SMI. Presarcopenia at baseline was not significantly associated with PFS. Conclusions: Decreased SMI was significantly associated with PFS. Monitoring muscle volume during atezolizumab plus bevacizumab therapy is useful in clinical practice.
Individualized Selection Criteria Based on Tumor Burden in Future Remnant Liver for Staged Hepatectomy of Advanced CRLM: Conventional TSH or ALPPS
Authors: Chan Kun-Ming , Hung Hao-Chien , Lee Jin-Chiao , Wu Tsung-Han , Wang Yu-Chao , Cheng Chih-Hsien , Lee Chen-Fang , Wu Ting-Jung , Chou Hong-Shiue , Lee Wei-Chen
Published: July 2022

Staged hepatectomy is a promising strategy for curative resection of advanced colorectal liver metastasis (CRLM) to prevent inadequate future remnant liver (FRL). However, the selection criteria for conventional two-stage hepatectomy (cTSH) and associating liver partitioning and portal vein ligation for staged hepatectomy (ALPPS) remain unclear. This study aimed to propose a selection criterion for determining the optimal staged hepatectomy for patients with advanced CRLM. A selection criterion based on the degree of metastatic tumors within the FRL was established to determine staged hepatectomy approaches. Generally, ALPPS is recommended for patients with ≤3 metastatic nodules and whose nodules do not measure >3 cm in the FRL. cTSH is performed for patients whose tumor burden in FRL beyond the selection criteria. Data of 37 patients who underwent staged hepatectomy and curative intent of CRLM were analyzed. The clinical characteristics and outcomes of the two approaches were compared. Overall, cTSH and ALPPS were performed for 27 (73.0%) and 10 (27.0%) patients, respectively. Of those, 20 patients in the cTSH group and all patients in the ALPPS group had completed staged hepatectomy. The 1-, 3-, and 5-year survival rates were 91.6%, 62.4%, and 45.4% for all patients, respectively. The outcomes of patients who had successfully completed the staged hepatectomy were significantly better than those of other patients who failed to achieve staged hepatectomy. However, no significant difference was observed in the overall survival of patients who underwent staged hepatectomy between the two groups, but those in the ALPPS group had 100% survival at the end of this study. The individualized selection criteria based on tumor burden in the FRL that could balance the operative risk and oncologic outcome appear to be a promising strategy for achieving complete staged hepatectomy in patients with advanced CRLM.
Quaternary Ammonium Salts Interact with Enolates and Sulfonates via Formation of Multiple +N-C-H Hydrogen Bonding Interactions
Authors: Bencivenni Grazia , Saraiva Rosa Nathalie , Grieco Paolo , Gillick-Healy Malachi W. , Kelly Brian G. , Twamley Brendan , Adamo Mauro F. A.
Published: July 2022

We report herein sharp physical evidence, i.e., single-crystal X-ray diffraction and 1H-NMR spectral data, confirming that quaternary ammonium species interact with anions via a set of directional ion–dipole cooperative +N-C-H unusual H-bonding interactions and not via pure non-directional ionic electrostatic interactions. This finding, which has been often invoked by calculations, is herein substantiated by the preparation of two model compounds and an analysis of their X-ray crystal structures in the solid state and 1H-NMR spectra in solution. These observations are particularly pertinent for the rational design of novel catalyses and catalysts and providing guidance to an understanding of these species in solution and during asymmetric enantioselective catalysis.
Cerebral Autoregulation Assessment Using the Near Infrared Spectroscopy ‘NIRS-Only’ High Frequency Methodology in Critically Ill Patients: A Prospective Cross-Sectional Study
Authors: Tas Jeanette , Eleveld Nick , Borg Melisa , Bos Kirsten D. J. , Langermans Anne P. , van Kuijk Sander M. J. , van der Horst Iwan C. C. , Elting Jan Willem J. , Aries Marcel J. H.
Published: July 2022

Impairments in cerebral autoregulation (CA) are related to poor clinical outcome. Near infrared spectroscopy (NIRS) is a non-invasive technique applied to estimate CA. Our general purpose was to study the clinical feasibility of a previously published ‘NIRS-only’ CA methodology in a critically ill intensive care unit (ICU) population and determine its relationship with clinical outcome. Bilateral NIRS measurements were performed for 1–2 h. Data segments of ten-minutes were used to calculate transfer function analyses (TFA) CA estimates between high frequency oxyhemoglobin (oxyHb) and deoxyhemoglobin (deoxyHb) signals. The phase shift was corrected for serial time shifts. Criteria were defined to select TFA phase plot segments (segments) with ‘high-pass filter’ characteristics. In 54 patients, 490 out of 729 segments were automatically selected (67%). In 34 primary neurology patients the median (q1–q3) low frequency (LF) phase shift was higher in 19 survivors compared to 15 non-survivors (13° (6.3–35) versus 0.83° (−2.8–13), p = 0.0167). CA estimation using the NIRS-only methodology seems feasible in an ICU population using segment selection for more robust and consistent CA estimations. The ‘NIRS-only’ methodology needs further validation, but has the advantage of being non-invasive without the need for arterial blood pressure monitoring.
Hedgehog Signaling in CNS Remyelination
Authors: Fang Minxi , Tang Tao , Qiu Mengsheng , Xu Xiaofeng
Published: July 2022

Remyelination is a fundamental repair process in the central nervous system (CNS) that is triggered by demyelinating events. In demyelinating diseases, oligodendrocytes (OLs) are targeted, leading to myelin loss, axonal damage, and severe functional impairment. While spontaneous remyelination often fails in the progression of demyelinating diseases, increased understanding of the mechanisms and identification of targets that regulate myelin regeneration becomes crucial. To date, several signaling pathways have been implicated in the remyelination process, including the Hedgehog (Hh) signaling pathway. This review summarizes the current data concerning the complicated roles of the Hh signaling pathway in the context of remyelination. We will highlight the open issues that have to be clarified prior to bringing molecules targeting the Hh signaling to demyelinating therapy.
The Association of Sugar-Sweetened Beverages to Children’s Weights Status Is Moderated by Frequency of Adding Sugars and Sleep Hours
Authors: Magriplis Emmanuella , Kanellopoulou Aikaterini , Notara Venetia , Antonogeorgos George , Rojas-Gil Andrea Paola , Kornilaki Ekaterina N , Lagiou Areti , Zampelas Antonis , Panagiotakos Demosthenes B
Published: July 2022

Adding extra sugars in milk and the moderating effect of sleep has yet to be investigated, setting the aim of this study. A total of 1361 school-aged children were included, aged 10–12 years old, by randomly sampling schools. Data were interview-obtained by trained personnel using a validated 17-item food frequency questionnaire, with specifics on type of milk and extra sugar additions. Analyses were stratified by average recommended hours of sleep. Predictive probability margins were obtained following necessary adjustments. Mean BMI was significantly lower, the level of SSB intake was higher, and the prevalence of adding sugars to milk four or more times per week was higher in children that slept ≥10 h. Most children (64%) consumed full fat milk, 21% had low fat, and 19.7% chocolate milk, with a significantly larger proportion of overweight or obese children consuming full fat or chocolate milk, also adding extra sugars four or more times per week (4.1% compared to 9.6%, and 12.2% compared to 39.5%, respectively). The predictive probability of being overweight or obese exponentially increased for children consuming >0.5 SSB/day while also adding sugars to their milk frequently, although this effect remained significant only for children sleeping <10 h/day. In conclusion, to accurately address the effect of SSBs on children’s body weight, frequency of any type of sugar addition in milk should be accounted for, as well as average sleep hours that may further moderate the effect.
Review of Radiation-Induced Effects on β-Ga2O3 Materials and Devices
Authors: Manikanthababu Nethala , Sheoran Hardhyan , Siddham Pradeep , Singh Rajendra
Published: July 2022

β-Ga2O3 has become an ultimate choice of emerging new-generation material for its wide range of compelling applications in power electronics. In this review, we have explored the available radiations in the atmosphere and the effects of radiation on the β-Ga2O3 material and devices. The focus in this review summarizes various studies covering different radiation such as swift heavy ions, protons, neutrons, electrons, Gamma, and X-rays to understand the radiation-induced effects on the structure and their reliable performance in harsh environments. In addition, we focused on the various pre-existing defects in β-Ga2O3 and the emergence of radiation-induced defects that provoke a severe concern, especially from the device performance point of view. This review presents the irradiation-induced effects on the devices such as high-power devices such as Schottky barrier diodes (SBDs), field-effect transistors (FETs), metal-oxide-semiconductor (MOS) devices, and photodetectors. Some key studies including the changes in carrier concentration with a removal rate, Schottky barrier height (SBH), ideality factor, defect dynamics dielectric damage, interface charge trapping, a thermally activated recovery mechanism for charge carriers at elevated temperature, and diffusion length of minority charge carriers. These reports show that β-Ga2O3-based devices could be deployable for space or high-radiation terrestrial applications. These results provide/suggest a better device design based on the radiation degradation studies in the state-of-the-art β-Ga2O3 devices.
Biosynthesis of Silver Nanoparticles Using Bersama engleriana Fruits Extracts and Their Potential Inhibitory Effect on Resistant Bacteria
Authors: Majoumouo Michele Stella , Tincho Marius Belmondo , Yimta Youmbi Diane , Adekiya Tayo Alex , Aruleba Raphael Taiwo , Ayawei Nimibofa , Boyom Fabrice Fekam , Morris Thureyah
Published: July 2022

The absence of novel, safe, and effective bactericide is an urgent concern worldwide and remains a challenge in scientific communities. The unique proprieties of silver nanoparticles (SNPs) synthesized from plant extracts make them a suitable candidate to overcome these limitations. Herein, we synthesized SNPs from Bersama engleriana fruit (BEfr) extracts and determined their potential antibacterial activity and mode of action. SNPs were synthesized from BEfr methanolic fruit extracts at 25 and 70 °C, and the antibacterial effectiveness of SNPs against bacterial strains was investigated. The surface plasmon resonance peaked at 430.18 and 434.08 nm, respectively, for SNPs synthesized at 25 and 70 °C, confirming SNPs synthesis. BEfr-SNPs had minimum inhibitory concentrations (MIC) range of 0.234 to >50 µg/mL, which was 30-fold greater than extract alone (MIC of 500 µg/mL). BEfr-SNPs-25 °C was potent against six bacterial strains (S. aureus, S. enterica, MRS. aureus, K. pneumonia, and S. pyogenes), with MIC range of 0.339 to 6.25 µg/mL. The mode of action of BEfr-SNPs-25 °C was achieved by an MRSA bacteria strain outer membrane and DNA nucleotide linkage. These results suggest that our synthesized SNPs, especially BEfr-SNPs-25 °C, demonstrated an enhanced antibacterial effect and could be potential candidates for bacterial infection treatment.
Rapid Automatized Picture Naming in an Outpatient Concussion Center: Quantitative Eye Movements during the Mobile Universal Lexicon Evaluation System (MULES) Test
Authors: Hudson Todd E. , Conway Jenna , Rizzo John-Ross , Martone John , Chou Liyung T. , Balcer Laura J. , Galetta Steven L. , Rucker Janet C.
Published: July 2022

Number and picture rapid automatized naming (RAN) tests are useful sideline diagnostic tools. The main outcome measure of these RAN tests is the completion time, which is prolonged with a concussion, yet yields no information about eye movement behavior. We investigated eye movements during a digitized Mobile Universal Lexicon Evaluation System (MULES) test of rapid picture naming. A total of 23 participants with a history of concussion and 50 control participants performed MULES testing with simultaneous eye tracking. The test times were longer in participants with a concussion (32.4 s [95% CI 30.4, 35.8] vs. 26.9 s [95% CI 25.9, 28.0], t=6.1). The participants with a concussion made more saccades per picture than the controls (3.6 [95% CI 3.3, 4.1] vs. 2.7 [95% CI 2.5, 3.0]), and this increase was correlated with longer MULES times (r = 0.46, p = 0.026). The inter-saccadic intervals (ISI) did not differ between the groups, nor did they correlate with the test times. Following a concussion, eye movement behavior differs during number versus picture RAN performance. Prior studies have shown that ISI prolongation is the key finding for a number-based RAN test, whereas this study shows a primary finding of an increased saccade number per picture with a picture-based RAN test. Number-based and picture-based RAN tests may be complimentary in concussion detection, as they may detect different injury effects or compensatory strategies.
Effects of Flood on Phytoplankton Diversity and Community Structure in Floodplain Lakes Connected to the Yangtze River
Authors: Wang Yutao , Fan Zhongya , Wang Wencai , Zhou Zhongze , Ye Xiaoxin
Published: July 2022

Flood events can significantly affect the physical and biological processes of aquatic ecosystems in a short time, leading to rapid changes in phytoplankton community structure. The Huayanghe Lakes experienced extreme flooding in the summer of 2020, with the water level reaching 16.42 m. In order to understand the effects of flooding on phytoplankton diversity and community structure, eight samples were collected in the Huayanghe Lakes from 2019 to 2020. Water-level disturbance has a significant influence on lake-water quality and phytoplankton community structure. The results showed that the Secchi depth increased from 65.36 to 8.52 cm, while the concentration of total nitrogen (from 0.98 to 0.7 mg/L) and total phosphorus (from 0.04 to 0.031 g/L) decreased. In addition, flooding significantly increased the Shannon–Wiener diversity index, the Pielou index, and the Margalef richness index by an average of 43.5%, 36.7%, and 40.21%, respectively. The phytoplankton community structure in the Huayanghe Lakes changed due to the change of physicochemical environment caused by flood. While in the pre-flood period phytoplankton was composed of large diatoms (e.g., Aulacoseira granulata), cyanobacteria (e.g., Microcystis sp., Anabaena sp., and Aphanizomenon sp.) and other multicellular taxa, the flood period showed an increase in the proportion of chlorophytes and diatoms that quickly adapted to settle in new environments. Pearson correlations and redundancy analyses showed that water level fluctuation was the most significant environmental factor affecting the phytoplankton community between the regular hydrological cycle and flood periods. There are few studies on phytoplankton in the Huayanghe Lakes, and the present study provides basic data on phytoplankton diversity and community structure. In addition, it provides a theoretical basis for controlling water level change in the Yangtze River.
Concept Development—From Academia to Industry: A Journey Motivated by the Design of Portable Thermal Slippers
Authors: Duarte Belmiro P. M. , Carvalho Ana M. F. , Lima Daniela S. B. , Costa Diana I. S. , Nunes Inês R. , Batista João P. S. C. , Gomes Paulo J. S. , Bernardo Fernando P.
Published: July 2022

Concept design is an early and crucial phase in product development where the general concept of a product is created. In this stage, various aspects of the product are considered, such as usability and technical performance, among others. Product design and development are currently a well-established area of knowledge, and academia is increasingly including its fundamentals in regular curricula, especially in the areas of engineering, design, and marketing. At the same time, industrial players recognize the potential of the method for i. responding successfully to market uncertainty and speed; ii. improving the knowledge available and the systematization of creative processes; and iii. ensuring that the decision process is explicit and well documented. Nevertheless, only a few studies demonstrating the concept design procedure in detail are available in the literature. In this paper, we thus present a detailed case study concerning the design of portable thermal slippers, so as to demonstrate the application of structured procedures and systematic approaches to concept design, and providing a pedagogical review of methods and tools to support the analysis and decisions along the several steps of the concept design procedure. The particular case study here addressed resulted from the collaboration between an academic institution and a company operating in the home footwear industry, with the design team including elements from both sides.
A Simple, Cost-Effective, and Extraction-Free Molecular Diagnostic Test for Sickle Cell Disease Using a Noninvasive Buccal Swab Specimen for a Limited-Resource Setting
Authors: Thakur Priya , Gupta Pragya , Bhargava Nupur , Soni Rajat , Varma Gottumukkala Narendra , Goswami Sangam Giri , Kharya Gaurav , Saravanakumar Vinodh , Gunda Padma , Jain Suman , Dass Jasmita , Aggarwal Mukul , Ramalingam Sivaprakash
Published: July 2022

Sickle cell disease (SCD) is the most prevalent life-threatening blood monogenic disorder. Currently, there is no cure available, apart from bone marrow transplantation. Early and efficient diagnosis of SCD is key to disease management, which would make considerable strides in alleviating morbidity and reducing mortality. However, the cost and complexity of diagnostic procedures, such as the Sanger sequencing method, impede the early detection of SCD in a resource-limited setting. To address this, the current study demonstrates a simple and efficient proof-of-concept assay for the detection of patients and carriers using extraction-free non-invasive buccal swab samples by isothermal DNA Amplification coupled Restrictase-mediated cleavage (iDAR). This study is a first of its kind reporting the use of buccal swab specimens for iDA in molecular diagnosis of a genetic disease, all the while being cost effective and time saving, with the total assay time of around 150 min at a cost of USD 5. Further, iDAR demonstrates 91.5% sensitivity and 100% specificity for detecting all three alleles: SS, AS, and AA, having a 100% concordance with Sanger sequencing. The applicability of the iDAR assay is further demonstrated with its adaptation to a one-pot reaction format, which simplifies the assay system. Overall, iDAR is a simple, cost-effective, precise, and non-invasive assay for SCD screening, with the potential for use in a limited resource setting.
Repeatability and Reproducibility of Retinal Fractal Dimension Measured with Swept-Source Optical Coherence Tomography Angiography in Healthy Eyes: A Proof-of-Concept Study
Authors: Arnould Louis , Haddad Déa , Baudin Florian , Gabrielle Pierre-Henry , Sarossy Marc , Bron Alain M. , Aliahmad Behzad , Creuzot-Garcher Catherine
Published: July 2022

The retinal vascular network fractal dimension (FD) could be a promising imaging biomarker. Our objective was to evaluate its repeatability and reproducibility in healthy eyes. A cross-sectional study was undertaken with young, healthy volunteers who had no reported cardiac risk factors or ocular disease history. For each participant, three SS-OCTA images (12 × 12 mm) were acquired using the Plex Elite 9000 (Carl Zeiss Meditec AG, Jena, Germany) by two ophthalmologists. Automated segmentation was obtained from both the superficial and deep capillary plexuses. FD was estimated by box counting. The intraclass correlation coefficients (ICC) were used as measures for repeatability and reproducibility. A total of 43 eyes of healthy volunteers were included. The mean ± standard deviation (SD) age was 30 ± 6.2 years. The results show good repeatability. The ICC was 0.722 (95% CI, 0.541–0.839) in the superficial capillary plexus and 0.828 (95% CI, 0.705–0.903) in the deep capillary plexus. For reproducibility, the ICC was 0.651 (95% CI, 0.439–0.795) and 0.363 (95% CI, 0.073–0.596) at the superficial and deep capillary plexus, respectively. In this study, the FD of the vascular network measured via SS-OCTA showed good repeatability and reproducibility in healthy participants.