bluuebunny/crossref_metadata_2025 huggingface dataset:
Age-Related Subgingival Colonization of Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis and Parvimonas micra—A Pragmatic Microbiological Retrospective Report
Authors: Claesson Rolf , Johansson Anders , Belibasakis Georgios N.
Published: May 2023

The aim of this study was to compare data about the prevalence and proportions of the bacterial species Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, and Parvimonas micra in periodontitis pocket samples collected from young, <35 years, and old, >35-year-old patients, YP and OP, respectively. The results from the analyses of a total of 3447 subgingival plaque samples analyzed for clinical diagnosis purposes by cultivation regarding the proportions of these species were collected from a database and elucidated. The prevalence of A. actinomycetemcomitans was found to be more than twice as high (OR = 2.96, 95% CI; 2.50–3.50) in samples from the younger (42.2%) than the older group (20.4%) (p < 0.001). The prevalence of P. micra was significantly lower in samples from the younger age group (OR = 0.43, 95%) (p < 0.001), whereas P. gingivalis was similarly distributed (OR = 0.78, 95%) in the two age groups (p = 0.006). A similar pattern was noticed for A. actinomycetemcomitans and P. gingivalis when high proportions (>50%) of the samples of these bacterial species were elucidated. In contrast, the proportion of samples containing >50% with P. micra was lower compared with the two other bacterial species. Furthermore, it was noted that the proportion of samples from old patients containing A. actinomycetemcomitans in combination with P. micra was almost three times higher than in samples when P. micra was replaced by P. gingivalis. In conclusion, A.actinomycetemcomitans showed an increased presence and proportion in samples from young patients compared with the old patients, while P. gingivalis was similarly distributed in the two age groups. P. micra showed an increased presence and proportion in samples from old patients compared with the young patients.
In Silico and In Vitro Methods in the Characterization of Beta-Carotene as Pharmaceutical Material via Acetylcholine Esterase Inhibitory Actions
Authors: Muthuraman Arunachalam , Ramesh Muthusamy , Mustaffa Fazlina , Nadeem Ahmed , Nishat Shamama , Paramakrishnan Nallupillai , Lim Khian Giap
Published: May 2023

Molecular docking is widely used in the assessment of the therapeutic potential of pharmaceutical agents. The binding properties of beta-carotene (BC) to acetylcholine esterase (AChE) proteins were characterized using the molecular docking method. The mechanism of AChE inhibition was assessed by an experimental in vitro kinetic study. In addition, the role of BC action was tested by the zebrafish embryo toxicity test (ZFET). The results of the docking ability of BC to AChE showed significant ligand binding mode. The kinetic parameter, i.e., the low AICc value shown as the compound was the competitive type of inhibition of AChE. Further, BC also showed mild toxicity at a higher dose (2200 mg/L) in ZFET assessment with changes in biomarkers. The LC50 value of BC is 1811.94 mg/L. Acetylcholine esterase (AChE) plays a pivotal role in the hydrolysis of acetylcholine, which leads to the development of cognitive dysfunction. BC possesses the regulation of acetylcholine esterase (AChE) and acid phosphatase (AP) activity to prevent neurovascular dysfunction. Therefore, the characterization of BC could be used as a pharmaceutical agent for the treatment of cholinergic neurotoxicity-associated neurovascular disorders such as developmental toxicity, vascular dementia, and Alzheimer’s disease due to its AChE and AP inhibitory actions.
Stretch-Induced Down-Regulation of HCN2 Suppresses Contractile Activity
Authors: Kola Job Baffin , Turarova Botagoz , Csige Dora , Sipos Ádám , Varga Luca , Gergely Bence , Refai Farah Al , Uray Iván P. , Docsa Tibor , Uray Karen
Published: May 2023

Although hyperpolarization-activated and cyclic nucleotide-gated 2 channels (HCN2) are expressed in multiple cell types in the gut, the role of HCN2 in intestinal motility is poorly understood. HCN2 is down-regulated in intestinal smooth muscle in a rodent model of ileus. Thus, the purpose of this study was to determine the effects of HCN inhibition on intestinal motility. HCN inhibition with ZD7288 or zatebradine significantly suppressed both spontaneous and agonist-induced contractile activity in the small intestine in a dose-dependent and tetrodotoxin-independent manner. HCN inhibition significantly suppressed intestinal tone but not contractile amplitude. The calcium sensitivity of contractile activity was significantly suppressed by HCN inhibition. Inflammatory mediators did not affect the suppression of intestinal contractile activity by HCN inhibition but increased stretch of the intestinal tissue partially attenuated the effects of HCN inhibition on agonist-induced intestinal contractile activity. HCN2 protein and mRNA levels in intestinal smooth muscle tissue were significantly down-regulated by increased mechanical stretch compared to unstretched tissue. Increased cyclical stretch down-regulated HCN2 protein and mRNA levels in primary human intestinal smooth muscle cells and macrophages. Overall, our results suggest that decreased HCN2 expression induced by mechanical signals, such as intestinal wall distension or edema development, may contribute to the development of ileus.
Optical Window to Polarity of Electrolyte Solutions
Authors: O’Mari Omar , Vullev Valentine I.
Published: May 2023

Medium polarity plays a crucial role in charge-transfer processes and electrochemistry. The added supporting electrolyte in electrochemical setups, essential for attaining the needed electrical conductivity, sets challenges for estimating medium polarity. Herein, we resort to Lippert–Mataga–Ooshika (LMO) formalism for estimating the Onsager polarity of electrolyte organic solutions pertinent to electrochemical analysis. An amine derivative of 1,8-naphthalimide proves to be an appropriate photoprobe for LMO analysis. An increase in electrolyte concentration enhances the polarity of the solutions. This effect becomes especially pronounced for low-polarity solvents. Adding 100 mM tetrabutylammonium hexafluorophosphate to chloroform results in solution polarity exceeding that of neat dichloromethane and 1,2-dichloroethane. Conversely, the observed polarity enhancement that emerges upon the same electrolyte addition to solvents such as acetonitrile and N,N-dimethylformamide is hardly as dramatic. Measured refractive indices provide a means for converting Onsager to Born polarity, which is essential for analyzing medium effects on electrochemical trends. This study demonstrates a robust optical means, encompassing steady-state spectroscopy and refractometry, for characterizing solution properties important for charge-transfer science and electrochemistry.
Synthesis, Crystal Structure, DFT Calculations, Hirshfeld Surface Analysis and In Silico Drug-Target Profiling of (R)-2-(2-(1,3-Dioxoisoindolin-2-yl)propanamido)benzoic Acid Methyl Ester
Authors: Rubab Syeda Laila , Raza Abdul Rauf , Nisar Bushra , Ashfaq Muhammad , Altaf Yasir , Hussain Riaz , Sajjad Noreen , Akram Muhammad Safwan , Tahir Muhammad Nawaz , Shaheen Muhammad Ashraf , Rehman Muhammad Fayyaz ur , Ali Hayssam M.
Published: May 2023

The work here reflects synthesis, DFT studies, Hirshfeld charge analysis and crystal data exploration of pharmacologically important (R)-2-(2-(1,3-dioxoisoindolin-2-yl)propanamido)benzoic acid methyl ester (5) to understand its properties for further chemical transformations. The methyl anthranilate (2) was produced by the esterification of anthranilic acid in an acidic medium. The phthaloyl-protected alanine (4) was rendered by the fusion of alanine with phthalic anhydride at 150 °C, followed by coupling with (2) furnished isoindole (5). The characterization of products was performed using IR, UV-Vis, NMR and MS. Single-crystal XRD also verified the structure of (5) in which N-H⋯O bonding stabilizes the molecular configuration of (5), resulting in the formation of S(6) hydrogen-bonded loop. The molecules of isoindole (5) are connected in the form of dimers, and the π⋯π stacking interaction between aromatic rings further stabilizes the crystal packing. DFT studies suggest that HOMO is over the substituted aromatic ring, the LUMO is present mainly over the indole side, and nucleophilic and electrophilic corners point out the reactivity of the product (5). In vitro and in silico analysis of (5) shows its potential as an antibacterial agent targeting DNA gyrase and Dihydroorotase from E. coli and tyrosyl-tRNA synthetase and DNA gyrase from Staphylococcus aureus.
Antibacterial and Cytocompatible pH-Responsive Peptide Hydrogel
Authors: Edirisinghe Dona Imanga Upamadi , D’Souza Areetha , Ramezani Maryam , Carroll Robert J. , Chicón Quenten , Muenzel Cheyene L. , Soule Jonathan , Monroe Mary Beth Browning , Patteson Alison E. , Makhlynets Olga V.
Published: May 2023

A short peptide, FHHF-11, was designed to change stiffness as a function of pH due to changing degree of protonation of histidines. As pH changes in the physiologically relevant range, G′ was measured at 0 Pa (pH 6) and 50,000 Pa (pH 8). This peptide-based hydrogel is antimicrobial and cytocompatible with skin cells (fibroblasts). It was demonstrated that the incorporation of unnatural AzAla tryptophan analog residue improves the antimicrobial properties of the hydrogel. The material developed can have a practical application and be a paradigm shift in the approach to wound treatment, and it will improve healing outcomes for millions of patients each year.
Syntheses and Electrochemical and EPR Studies of Porphyrins Functionalized with Bulky Aromatic Amine Donors
Authors: Carvalho Mary-Ambre , Merahi Khalissa , Haumesser Julien , Pereira Ana Mafalda Vaz Martins , Parizel Nathalie , Weiss Jean , Orio Maylis , Maurel Vincent , Ruhlmann Laurent , Choua Sylvie , Ruppert Romain
Published: May 2023

A series of nickel(II) porphyrins bearing one or two bulky nitrogen donors at the meso positions were prepared by using Ullmann methodology or more classical Buchwald–Hartwig amination reactions to create the new C-N bonds. For several new compounds, single crystals were obtained, and the X-ray structures were solved. The electrochemical data of these compounds are reported. For a few representative examples, spectroelectrochemical measurements were used to clarify the electron exchange process. In addition, a detailed electron paramagnetic resonance (EPR) study was performed to estimate the extent of delocalization of the generated radical cations. In particular, electron nuclear double resonance spectroscopy (ENDOR) was used to determine the coupling constants. DFT calculations were conducted to corroborate the EPR spectroscopic data.
A Modified Protocol for the Isolation, Culture, and Characterization of Human Smooth Muscle Cells from the Umbilical Cord
Authors: Ibrahim Asmaa Q. , Abdullah Mohammed S. , Ahram Mamoun , Abdalla Shtaywy
Published: May 2023

Background: Vascular smooth muscle cells (VSMCs) and vascular endothelial cells are key participants in the pathogenesis of atherosclerosis. Human umbilical vein endothelial cells (HUVECs) and VSMCs are useful models to design therapeutic strategies for many cardiovascular diseases (CVDs). However, procuring a VSMC cell line by researchers, to model atherosclerosis, for example, is impeded by time and cost limitations, as well as by many other logistic problems in many countries. Results: This article describes a protocol for the quick and cheap isolation of VSMCs from human umbilical cords using a mechanical and enzymatic method. This VSMC protocol yields a confluent primary culture that could be obtained within 10 days and sub-cultured for 8–10 passages. The isolated cells are characterized by their morphology and the expression of mRNA of marker proteins analyzed by reverse transcription polymerase chain reaction (RT-qPCR). Conclusion: The protocol described herein for the isolation of VSMCs from human umbilical cords is easy and is time- and cost-efficient. Isolated cells are useful models for understanding the mechanisms underlying many pathophysiological conditions.
On-Slide Lambda Protein Phosphatase-Mediated Dephosphorylation of Fixed Samples
Authors: Tishchenko Alexander , Van Waesberghe Cliff , Favoreel Herman W.
Published: May 2023

Protein phosphorylation is a ubiquitous post-translational modification that regulates a plethora of intracellular processes, making its analysis crucial for understanding intracellular dynamics. The commonly used methods, such as radioactive labeling and gel electrophoresis, do not provide information about subcellular localization. Immunofluorescence using phospho-specific antibodies and subsequent analysis via microscopy allows researchers to assess subcellular localization, but it typically lacks validation whether the observed fluorescent signal is phosphorylation specific. In this study, an on-slide dephosphorylation assay coupled with immunofluorescence staining using phospho-specific antibodies on fixed samples is proposed as a fast and simple approach to validate phosphorylated proteins in their native subcellular context. The assay was validated using antibodies against two different phosphorylated target proteins, connexin 43 phosphorylated at serine 373, and phosphorylated substrates of protein kinase A, with a dramatic reduction in the signal upon dephosphorylation. The proposed approach provides a convenient way to validate phosphorylated proteins without the need for additional sample preparation steps, reducing the time and effort required for analysis, while minimizing the risk of protein loss or alteration.
Similarities and Differences between Immersive Virtual Reality, Real World, and Computer Screens: A Systematic Scoping Review in Human Behavior Studies
Authors: Hepperle Daniel , Wölfel Matthias
Published: May 2023

In the broader field of human behavior studies, there are several trade-offs for on-site experiments. To be tied to a specific location can limit both the availability and diversity of participants. However, current and future technological advances make it possible to replicate real-world scenarios in a virtual environment up to a certain level of detail. How these differences add up and affect the cross-media validity of findings remains a topic of debate. How a virtual world is accessed, through a computer screen or a head-mounted display, may have a significant impact. Not surprisingly, the literature has presented various comparisons. However, while previous research has compared the different devices for a specific research question, a systematic review is lacking. To fill this gap, we conducted this review. We identified 1083 articles in accordance with the PRISMA guidelines. Following screening, 56 articles remained and were compared for a qualitative synthesis to provide the reader a summary of current research on the differences between head-mounted displays (HMDs), computer screens, and the real world. Overall, the data show that virtual worlds presented in an HMD are more similar to real-world situations than to computer screens. This supports the thesis that HMDs are more suitable than computer screens for conducting experiments in the field of human behavioral studies.
A Digital Coach to Promote Emotion Regulation Skills
Authors: Hopman Katherine , Richards Deborah , Norberg Melissa M.
Published: May 2023

There is growing awareness that effective emotion regulation is critical for health, adjustment and wellbeing. Emerging evidence suggests that interventions that promote flexible emotion regulation may have the potential to reduce the incidence and prevalence of mental health problems in specific at-risk populations. The challenge is how best to engage with at risk populations, who may not be actively seeking assistance, to deliver this early intervention approach. One possible solution is via digital technology and development, which has rapidly accelerated in this space. Such rapid growth has, however, occurred at the expense of developing a deep understanding of key elements of successful program design and specific mechanisms that influence health behavior change. This paper presents a detailed description of the design, development and evaluation of an emotion regulation intervention conversational agent (ERICA) who acts as a digital coach. ERICA uses interactive conversation to encourage self-reflection and to support and empower users to learn a range of cognitive emotion regulation strategies including Refocusing, Reappraisal, Planning and Putting into Perspective. A pilot evaluation of ERICA was conducted with 138 university students and confirmed that ERICA provided a feasible and highly usable method for delivering an emotion regulation intervention. The results also indicated that ERICA was able to develop a therapeutic relationship with participants and increase their intent to use a range of cognitive emotion regulation strategies. These findings suggest that ERICA holds potential to be an effective approach for delivering an early intervention to support mental health and wellbeing. ERICA’s dialogue, embedded with interactivity, therapeutic alliance and empathy cues, provide the basis for the development of other psychoeducation interventions.
Effect of Higher Order Aberrations and Intraocular Scatter on Optical Quality Based on an Optical Eye Model
Authors: Rao Feng , Zhao Xing Heng , Zhang Ming Dong , Wang Yan
Published: May 2023

The impact of intraocular scatter and higher order aberrations (HOAs) on ocular optical quality was investigated. An optical eye model was constructed using the measured ocular aberrations, corneal surfaces, axial length, and scatter fraction, and the impact of HOAs and scatter on modulation transfer functions (MTFs) was studied based on the newly established optical eye model. For uniform intraocular scatter, the monochromatic and polychromatic MTF decreased as the HOAs or scatter fractions increased independently at each spatial frequency, which implied that both were essential for visual quality. In addition, the scatter effect on MTF was more significant for the eye with less HOA. The combined deterioration effect of these two factors on the MTF was less than their summation, suggesting a potential compensatory mechanism between HOAs and scatter.
Parameters and Pulsation Constant of Cepheid
Authors: Sinitsyn Sergei V.
Published: May 2023

The analysis of fifty empirical period-radius relations and forty-three empirical period-luminosity relations is performed for the Cepheids. It is found that most of these relations have significant systematic errors. A new metrological method is suggested to exclude these systematic errors using the new empirical metrological relations and the empirical temperature scale of the various samples of the Cepheids. In this regard, the reliable relations between the mass, radius, effective surface temperature, luminosity, absolute magnitude on the one hand, and the pulsation period on the other hand, as well as the reliable dependence of the radius on the mass are determined for the Cepheids of types δ Cephei and δ Scuti from the Galaxy. These reliable relations permit us to accurately determine the empirical value of the pulsation constant for the Cepheids of both types for the first time. It is found that the pulsation constant very weakly depends on the pulsation period of the Cepheid, contrary to the known theoretical calculation. Hence, the Cepheids pulsate almost as a unified whole and homogeneous spherical body in wide ranges of a star’s mass and evolutionary state with an extremely inhomogeneous distribution of stellar substance over its volume. Therefore, it is first suggested that the pulsation of the Cepheid is, first of all, the pulsation of the almost unified whole and homogenous shell of its gravitational mass. This pulsation is triggered by well-known effects; for example, the local optical opacity of the stellar substance and overshooting, using the usual pulsation of the stellar substance.
Leishmaniosis in Greece: The Veterinary Perspective
Authors: Symeonidou Isaia , Sioutas Georgios , Gelasakis Athanasios I. , Tsokana Constantina N. , Papadopoulos Elias
Published: May 2023

Leishmaniosis caused by the protozoon Leishmania infantum that is transmitted through the bites of infected phlebotomine sandflies is of major veterinary concern in Greece. The country is endemic with particularly favourable environmental conditions for the spread of this infection. Moreover, Greece remains a popular touristic destination, and the continuous travel of pets raises concern regarding the possible dissemination of infection from endemic to non-endemic areas. Dogs are the main reservoir host, although other animal species, including humans, may also be infected. Canine leishmaniosis manifests as a visceral disease that can result in death if left untreated. Serological and molecular epizootiological studies have confirmed circulation of the parasite in Greek canine and feline populations as well as in other mammals. As a result, constant surveillance and identification of high-risk localities are necessary to establish chemoprophylactic protocols for travelling animals to safeguard animal and public health.
Design of Optimal SPR-Based Multimode Waveguide Sensor for a Wide Range of Liquid Analytes
Authors: Feiler Martin , Ziman Martin , Kovac Jaroslav , Kuzma Anton , Uherek Frantisek
Published: May 2023

Surface plasmon resonance (SPR)-based sensors have attracted huge interest due to their ability to provide real-time sensing of a wide range of quantities, while still boasting high sensitivity, low cost, and electromagnetic immunity. Among various types of these sensors, waveguide-based sensors show great promise in terms of integrability into more complex circuits while still maintaining similar sensitivity and versatility as their stand-alone counterparts. This paper presents the results of investigation based on design and simulation of optimal parameters for multimode SPR-based waveguide sensor sensitive to any commonly used liquid analytes. The sensitivity for a wider range of analytes is achieved by utilisation of SiON material platform because refractive index adjustability of SiON offers a way to tailor sensor’s sensitivity to exact applications and analytes. One of the research goals in this work was to show that multi-mode waveguides may be suitable for such sensors with comparable sensitivity and higher transmission rates as single-mode waveguides. To showcase design possibilities, two variations with the same design and material’s refractive index adjustment only were investigated, and their optimal parameters have been found. Sensitivity for all commonly used liquid analytes was validated, with sensitivity rising to 1400 nm/RIU for label-free refractive index sensing, even without the use of any sensitivity-enhancing structures.
Virus-Induced Gene Silencing (VIGS) in Chinese Jujube
Authors: Zhang Yao , Niu Nazi , Li Shijia , Liu Yin , Xue Chaoling , Wang Huibin , Liu Mengjun , Zhao Jin
Published: May 2023

Virus-induced gene silencing (VIGS) is a fast and efficient method for assaying gene function in plants. At present, the VIGS system mediated by Tobacco rattle virus (TRV) has been successfully practiced in some species such as cotton and tomato. However, little research of VIGS systems has been reported in woody plants, nor in Chinese jujube. In this study, the TRV-VIGS system of jujube was firstly investigated. The jujube seedlings were grown in a greenhouse with a 16 h light/8 h dark cycle at 23 °C. After the cotyledon was fully unfolded, Agrobacterium mixture containing pTRV1 and pTRV2-ZjCLA with OD600 = 1.5 was injected into cotyledon. After 15 days, the new leaves of jujube seedlings showed obvious photo-bleaching symptoms and significantly decreased expression of ZjCLA, indicating that the TRV-VIGS system had successfully functioned on jujube. Moreover, it found that two injections on jujube cotyledon could induce higher silencing efficiency than once injection. A similar silencing effect was then also verified in another gene, ZjPDS. These results indicate that the TRV-VIGS system in Chinese jujube has been successfully established and can be applied to evaluate gene function, providing a breakthrough in gene function verification methods.
Contribution of Arbuscular Mycorrhizal and Endophytic Fungi to Drought Tolerance in Araucaria araucana Seedlings
Authors: Chávez Daniel , Rivas Gustavo , Machuca Ángela , Santos Cledir , Deramond Christian , Aroca Ricardo , Cornejo Pablo
Published: May 2023

In its natural distribution, Araucaria araucana is a plant species usually exposed to extreme environmental constraints such as wind, volcanism, fires, and low rainfall. This plant is subjected to long periods of drought, accentuated by the current climate emergency, causing plant death, especially in its early growth stages. Understanding the benefits that both arbuscular mycorrhizal fungi (AMF) and endophytic fungi (EF) could provide plants under different water regimes would generate inputs to address the above-mentioned issues. Here, the effect of AMF and EF inoculation (individually and combined) on the morphophysiological variables of A. araucana seedlings subjected to different water regimes was evaluated. Both the AMF and EF inocula were obtained from A. araucana roots growing in natural conditions. The inoculated seedlings were kept for 5 months under standard greenhouse conditions and subsequently subjected to three different irrigation levels for 2 months: 100, 75, and 25% of field capacity (FC). Morphophysiological variables were evaluated over time. Applying AMF and EF + AMF yielded a noticeable survival rate in the most extreme drought conditions (25% FC). Moreover, both the AMF and the EF + AMF treatments promoted an increase in height growth between 6.1 and 16.1%, in the production of aerial biomass between 54.3 and 62.6%, and in root biomass between 42.5 and 65.4%. These treatments also kept the maximum quantum efficiency of PSII (Fv/Fm 0.71 for AMF and 0.64 for EF + AMF) stable, as well as high foliar water content (>60%) and stable CO2 assimilation under drought stress. In addition, the EF + AMF treatment at 25% FC increased the total chlorophyll content. In conclusion, using indigenous strains of AMF, alone or in combination with EF, is a beneficial strategy to produce A. araucana seedlings with an enhanced ability to tolerate prolonged drought periods, which could be of great relevance for the survival of these native species under the current climate change.
Convergent Adaptation of Multiple Herbicide Resistance to Auxin Mimics and ALS- and EPSPS-Inhibitors in Brassica rapa from North and South America
Authors: Dominguez-Valenzuela José Alfredo , Palma-Bautista Candelario , Vazquez-Garcia José G. , Yanniccari Marcos , Gigón Ramón , Alcántara-de la Cruz Ricardo , De Prado Rafael , Portugal João
Published: May 2023

Herbicide-resistant weeds have been identified and recorded on every continent where croplands are available. Despite the diversity of weed communities, it is of interest how selection has led to the same consequences in distant regions. Brassica rapa is a widespread naturalized weed that is found throughout temperate North and South America, and it is a frequent weed among winter cereal crops in Argentina and in Mexico. Broadleaf weed control is based on glyphosate that is used prior to sowing and sulfonylureas or mimic auxin herbicides that are used once the weeds have already emerged. This study was aimed at determining whether a convergent phenotypic adaptation to multiple herbicides had occurred in B. rapa populations from Mexico and Argentina by comparing the herbicide sensitivity to inhibitors of the acetolactate synthase (ALS), 5-enolpyruvylshikimate-3-phosphate (EPSPS), and auxin mimics. Five B. rapa populations were analyzed from seeds collected in wheat fields in Argentina (Ar1 and Ar2) and barley fields in Mexico (Mx1, Mx2 and MxS). Mx1, Mx2, and Ar1 populations presented multiple resistance to ALS- and EPSPS-inhibitors and to auxin mimics (2,4-D, MCPA, and fluroxypyr), while the Ar2 population showed resistance only to ALS-inhibitors and glyphosate. Resistance factors ranged from 947 to 4069 for tribenuron-methyl, from 1.5 to 9.4 for 2,4-D, and from 2.7 to 42 for glyphosate. These were consistent with ALS activity, ethylene production, and shikimate accumulation analyses in response to tribenuron-methyl, 2,4-D, and glyphosate, respectively. These results fully support the evolution of the multiple- and cross-herbicide resistance to glyphosate, ALS-inhibitors, and auxinic herbicides in B. rapa populations from Mexico and Argentina.
Nitric Oxide, a Key Modulator in the Alleviation of Environmental Stress-Mediated Damage in Crop Plants: A Meta-Analysis
Authors: Khan Murtaza , Al Azzawi Tiba Nazar Ibrahim , Ali Sajid , Yun Byung-Wook , Mun Bong-Gyu
Published: May 2023

Nitric oxide (NO) is a small, diatomic, gaseous, free radicle, lipophilic, diffusible, and highly reactive molecule with unique properties that make it a crucial signaling molecule with important physiological, biochemical, and molecular implications for plants under normal and stressful conditions. NO regulates plant growth and developmental processes, such as seed germination, root growth, shoot development, and flowering. It is also a signaling molecule in various plant growth processes, such as cell elongation, differentiation, and proliferation. NO also regulates the expression of genes encoding hormones and signaling molecules associated with plant development. Abiotic stresses induce NO production in plants, which can regulate various biological processes, such as stomatal closure, antioxidant defense, ion homeostasis, and the induction of stress-responsive genes. Moreover, NO can activate plant defense response mechanisms, such as the production of pathogenesis-related proteins, phytohormones, and metabolites against biotic and oxidative stressors. NO can also directly inhibit pathogen growth by damaging their DNA and proteins. Overall, NO exhibits diverse regulatory roles in plant growth, development, and defense responses through complex molecular mechanisms that still require further studies. Understanding NO’s role in plant biology is essential for developing strategies for improved plant growth and stress tolerance in agriculture and environmental management.
Identification and Expression Analysis of Phosphatidylinositol Transfer Proteins Genes in Rice
Authors: Pei Mengtian , Xie Xuze , Peng Baoyi , Chen Xinchi , Chen Yixuan , Li Ya , Wang Zonghua , Lu Guodong
Published: May 2023

The family of phosphatidylinositol transfer proteins (PITPs) is able to bind specific lipids to carry out various biological functions throughout different stages of plant life. But the function of PITPs in rice plant is unclear. In this study, 30 PITPs were identified from rice genome, which showed differences in physicochemical properties, gene structure, conservation domains, and subcellular localization. The promoter region of the OsPITPs genes included at least one type of hormone response element, such as methyl jasmonate (Me JA) and salicylic acid (SA). Furthermore, the expression level of OsML-1, OsSEC14-3, OsSEC14-4, OsSEC14-15, and OsSEC14-19 genes were significantly affected by infection of rice blast fungus Magnaporthe oryzae. Based on these findings, it is possible that OsPITPs may be involved in rice innate immunity in response to M. oryzae infection through the Me JA and SA pathway.
Leaf Color Classification and Expression Analysis of Photosynthesis-Related Genes in Inbred Lines of Chinese Cabbage Displaying Minor Variations in Dark-Green Leaves
Authors: Su Xiangjie , Yue Xiaonan , Kong Mingyu , Xie Ziwei , Yan Jinghui , Ma Wei , Wang Yanhua , Zhao Jianjun , Zhang Xiaomeng , Liu Mengyang
Published: May 2023

The leaves of the Chinese cabbage which is most widely consumed come in a wide variety of colors. Leaves that are dark green can promote photosynthesis, effectively improving crop yield, and therefore hold important application and cultivation value. In this study, we selected nine inbred lines of Chinese cabbage displaying slight differences in leaf color, and graded the leaf color using the reflectance spectra. We clarified the differences in gene sequences and the protein structure of ferrochelatase 2 (BrFC2) among the nine inbred lines, and used qRT-PCR to analyze the expression differences of photosynthesis-related genes in inbred lines with minor variations in dark-green leaves. We found expression differences among the inbred lines of Chinese cabbage in photosynthesis-related genes involved in the porphyrin and chlorophyll metabolism, as well as in photosynthesis and photosynthesis-antenna protein pathway. Chlorophyll b content was significantly positively correlated with the expression of PsbQ, LHCA1_1 and LHCB6_1, while chlorophyll a content was significantly negatively correlated with the expression PsbQ, LHCA1_1 and LHCA1_2. Our results provide an empirical basis for the precise identification of candidate genes and a better understanding of the molecular mechanisms responsible for the production of dark-green leaves in Chinese cabbage.
Protective Role of an Extract Waste Product from Citrus bergamia in an In Vitro Model of Neurodegeneration
Authors: Maiuolo Jessica , Bosco Francesca , Guarnieri Lorenza , Nucera Saverio , Ruga Stefano , Oppedisano Francesca , Tucci Luigi , Muscoli Carolina , Palma Ernesto , Giuffrè Angelo Maria , Mollace Vincenzo
Published: May 2023

A balanced diet, rich in fruits and vegetables and ensuring the intake of natural products, has been shown to reduce or prevent the occurrence of many chronic diseases. However, the choice to consume large quantities of fruits and vegetables leads to an increase in the amount of waste, which can cause an alteration in environmental sustainability. To date, the concept of a “byproduct” has evolved, now being understood as a waste product from which it is still possible obtain useful compounds. Byproducts in the agricultural sector are a rich source of bioactive compounds, capable of possessing a second life, decreasing the amount of waste products, the disposal costs, and environmental pollution. A promising and well-known citrus of the Mediterranean diet is the bergamot (Citrus bergamia, Risso et Poiteau). The composition of bergamot is known, and the rich presence of phenolic compounds and essential oils has justified the countless beneficial properties found, including anti-inflammatory, antioxidant, anti-cholesterolemic, and protective activity for the immune system, heart failure, and coronary heart diseases. The industrial processing of bergamot fruits leads to the formation of bergamot juice and bergamot oil. The solid residues, referred to as “pastazzo”, are normally used as feed for livestock or pectin production. The fiber of bergamot (BF) can be obtained from pastazzo and could exert an interesting effect thanks to its content of polyphenols. The aims of this work were twofold: (a) to have more information (composition, polyphenol and flavonoid content, antioxidant activity, etc.) on BF powder and (b) to verify the effects of BF on an in vitro model of neurotoxicity induced by treatment with amyloid beta protein (Aβ). In particular, a study of cell lines was carried out on both neurons and oligodendrocytes, to measure the involvement of the glia and compare it with that of the neurons. The results obtained showed that BF powder contains polyphenols and flavonoids and that it is able to exercise an antioxidant property. Moreover, BF exerts a protective action on the damage induced by treatment with Aβ, and this defense is found in experiments on the cell viability, on the accumulation of reactive oxygen species, on the involvement of the expression of caspase-3, and on necrotic or apoptotic death. In all these results, oligodendrocytes were always more sensitive and fragile than neurons. Further experiments are needed, and if this trend is confirmed, BF could be used in AD; at the same time, it could help to avoid the accumulation of waste products.
Assessment of Symptoms of Grapevine Leafroll Disease and Relationship with Yield and Quality of Pinot Noir Grape Must in a 10-Year Study Period
Authors: Cabaleiro Cristina , Pesqueira Ana M. , García-Berrios Julián J.
Published: May 2023

Grapevine leafroll disease (GLD) is caused by one or more of the Grapevine leafroll-associated viruses (GLRaVs). GLD’s symptoms are expected to be evident in indicator cultivars, regardless of the GLRaV(s) involved. In the present study, disease incidence (I) and severity (S), symptoms before veraison (Sy < V), a disease severity index (DSI) and an earliness index (EI) (2013–2022) were recorded in order to examine the factors affecting the evolution of GLD in Pinot noir graft inoculated with scions infected with GLRaV-3 that, in origin, showed a diversity of GLD symptoms. Strong correlations between I and S (r = 0.94) and between Sy < V and EI (r = 0.94) were observed; early symptoms proved good predictors of incidence and severity after veraison and of yield and sugar content of the must. The environmental conditions and time after infection did not modify the wide range of symptoms (I: 0–81.5%; S: 0.1–4) that corresponded with the variation in losses (<0–88% for yield and <0–24% for sugar content). With all other factors being constant, the significant differences between plants were mainly due to the GLRaVs present. Plants infected with some GLRaV-3 isolates always had mild symptoms or remained asymptomatic 10 years after grafting but remained a source of infection for GLRaV vectors.
Sanguisorba minor Scop.: An Overview of Its Phytochemistry and Biological Effects
Authors: Tocai (Moţoc) Alexandra Cristina , Kokeric Tijana , Tripon Septimiu , Barbu-Tudoran Lucian , Barjaktarevic Ana , Cupara Snezana , Vicas Simona Ioana
Published: May 2023

Since ancient times, many plants have been cultivated for their nutritional and medicinal properties. The genus Sanguisorba has been used for medicinal purposes for more than 2000 years. These species are distributed in temperate, arctic, or alpine areas in the Northern Hemisphere. Elongated, imparipinnate leaves and densely clustered flower heads are characteristics of the genus Sanguisorba. While Sanguisorba officinalis L. is mainly known for its significant medicinal applications, Sanguisorba minor Scop. is beginning to attract greater interest for its chemical composition and biological effects. Our research collected extensive information on Sanguisorba minor, including its history, taxonomy, habitat, and distribution, as well as its bioactive components and biological activities. In addition to electron microscopy of plant parts (root, stems, and leaves), which is described for the first time in the literature in the case of S. minor, the study also provides information on potential pests or beneficial insects that may be present. Our goal was to provide important information that will serve as a solid foundation for upcoming research on Sanguisorba minor Scop.
Weed Species from Tea Gardens as a Source of Novel Aluminum Hyperaccumulators
Authors: Hajiboland Roghieh , Moradi Aiuob , Kahneh Ehsan , Poschenrieder Charlotte , Nazari Fatemeh , Pavlovic Jelena , Tolra Roser , Salehi-Lisar Seyed-Yahya , Nikolic Miroslav
Published: May 2023

Increased availability of toxic Al3+ is the main constraint limiting plant growth on acid soils. Plants adapted to acid soils, however, tolerate toxic Al3+, and some can accumulate Al in their aerial parts to a significant degree. Studies on Al-tolerant and Al-accumulating species have mainly focused on the vegetation of acid soils distributed as two global belts in the northern and southern hemispheres, while acid soils formed outside these regions have been largely neglected. The acid soils (pH 3.4–4.2) of the tea plantations in the south Caspian region of Northern Iran were surveyed over three seasons at two main locations. Aluminum and other mineral elements (including nutrients) were measured in 499 plant specimens representing 86 species from 43 families. Al accumulation exceeding the criterion for accumulator species (>1000 µg g−1 DW) was found in 36 species belonging to 23 families of herbaceous annual or perennial angiosperms, in addition to three bryophyte species. Besides Al, Fe accumulation (1026–5155 µg g−1 DW) was also observed in the accumulator species that exceeded the critical toxicity concentration, whereas no such accumulation was observed for Mn. The majority of analyzed accumulator plants (64%) were cosmopolitan or pluriregional species, with a considerable rate of Euro-Siberian elements (37%). Our findings, which may contribute to phylogenetic studies of Al accumulators, also suggest suitable accumulator and excluder species for the rehabilitation of acid-eroded soils and introduce new model species for investigating Al accumulation and exclusion mechanisms.
Plasma Treatment of Polystyrene Films—Effect on Wettability and Surface Interactions with Au Nanoparticles
Authors: Islam Mohammad , Matouk Zineb , Ouldhamadouche Nadir , Pireaux Jean-Jacques , Achour Amine
Published: May 2023

Polystyrene (PS)/Gold (Au) is used for a wide range of applications, including composite nanofibers, catalysis, organic memory devices, and biosensing. In this work, PS films were deposited on silicon substrates via a spin coating technique followed by treatment with argon (Ar) plasma admixed with ammonia (NH3), oxygen (O2), or tetrafluoroethane (C2H2F4). X-Ray photoelectron spectroscopy (XPS) analysis revealed modified surface chemistry for Ar/O2, Ar/NH3, or Ar/C2H2F4 plasma treatment through the incorporation of oxygen, nitrogen, or fluorine groups, respectively. Size-controlled magnetron sputter deposition of Au nanoparticles (NP) onto these plasma-treated PS films was investigated via XPS and AFM techniques. The interaction of the Au NPs, as probed from the XPS and AFM measurements, is discussed by referring to changes in surface chemistry and morphology of the PS after plasma treatment. The results demonstrate the effect of surface chemistry on the interaction of Au NPs with polymer support having different surface functionalities. The XPS results show that significant oxygen surface incorporation resulted from oxygen-containing species in the plasma itself. The surface concentration of O increased from 0.4% for the pristine PS to 4.5 at%, 35.4 at%, and 45.6 at% for the Ar/C2H4F4, Ar/NH3, and Ar/O2, respectively. The water contact angle (WCA) values were noticed to decrease from 98° for the untreated PS to 95°, 37°, and 15° for Ar/C2H2F4, Ar/NH3, and Ar/O2 plasma-modified PS samples, respectively. AFM results demonstrate that surface treatment was also accompanied by surface morphology change. Small Au islands are well dispersed and cover the surface, thus forming a homogeneous, isotropic structure. The reported results are important for exploiting Au NPs use in catalysis and sensing applications.
New Approaches to the Synthesis and Stabilization of Polymer Microspheres with a Narrow Size Distribution
Authors: Gritskova Inessa A. , Prokopov Nikolay I. , Ezhova Anna A. , Chalykh Anatoly E. , Gusev Sergey A. , Levachev Sergey M. , Zubov Vitaly P. , Gomzyak Vitaly I. , Skopintsev Ivan V. , Stuzhuk Alexander N. , Kovtun Ivan D. , Shulgin Anton M. , Ivashkevich Dmitry S. , Romanenko German A. , Lakhtin Valentin G. , Chvalun Sergei N.
Published: May 2023

This article presents the results of investigations on heterophase polymerization of vinyl monomers in the presence of organosilicon compounds of different structures. On the basis of the detailed study of the kinetic and topochemical regularities of the heterophase polymerization of vinyl monomers, the conditions for the synthesis of polymer suspensions with a narrow particle-size distribution using a one-step method have been determined.
Design and Synthesis of N-Doped Porous Carbons for the Selective Carbon Dioxide Capture under Humid Flue Gas Conditions
Authors: Abdelnaby Mahmoud M. , Aliyu Mansur , Nemitallah Medhat A. , Alloush Ahmed M. , Mahmoud El-Hassan M. , Ossoss Khaled M. , Zeama Mostafa , Dowaidar Moataz
Published: May 2023

The design of novel porous solid sorbents for carbon dioxide capture is critical in developing carbon capture and storage technology (CCS). We have synthesized a series of nitrogen-rich porous organic polymers (POPs) from crosslinking melamine and pyrrole monomers. The final polymer’s nitrogen content was tuned by varying the melamine ratio compared to pyrrole. The resulting polymers were then pyrolyzed at 700 °C and 900 °C to produce high surface area nitrogen-doped porous carbons (NPCs) with different N/C ratios. The resulting NPCs showed good BET surface areas reaching 900 m2 g−1. Owing to the nitrogen-enriched skeleton and the micropore nature of the prepared NPCs, they exhibited CO2 uptake capacities as high as 60 cm3 g−1 at 273 K and 1 bar with significant CO2/N2 selectivity. The materials showed excellent and stable performance over five adsorption/desorption cycles in the dynamic separation of the ternary mixture of N2/CO2/H2O. The method developed in this work and the synthesized NPCs’ performance towards CO2 capture highlight the unique properties of POPs as precursors for synthesizing nitrogen-doped porous carbons with a high nitrogen content and high yield.
Artificial Neural Network Model for Temperature Prediction and Regulation during Molten Steel Transportation Process
Authors: Fang Linfang , Su Fuyong , Kang Zhen , Zhu Haojun
Published: May 2023

With the continuous optimization of the steel production process and the increasing emergence of smelting methods, it has become difficult to monitor and control the production process using the traditional steel management model. The regulation of steel smelting processes by means of machine learning has become a hot research topic in recent years. In this study, through the data mining and correlation analysis of the main equipment and processes involved in steel transfer, a network algorithm was optimized to solve the problems of standard back propagation (BP) networks, and a steel temperature forecasting model based on improved back propagation (BP) neural networks was established for basic oxygen furnace (BOF) steelmaking, ladle furnace (LF) refining, and Ruhrstahl–Heraeus (RH) refining. The main factors influencing steel temperature were selected through theoretical analysis and heat balance principles; the production data were analyzed; and the neural network was trained and tested using large amounts of field data to predict the end-point steel temperature of basic oxygen furnace (BOF) steelmaking, ladle furnace (LF) refining, and Ruhrstahl–Heraeus (RH) refining. The prediction model was applied to predict the degree of influence of different operating parameters on steel temperature. A comparison of the prediction results with the production data shows that the prediction system has good prediction accuracy, with a hit rate of over 90% for steel temperature deviations within 20 °C. Compared with the traditional steel temperature management model, the prediction system in this paper has higher management efficiency and a faster response time and is more practical and generalizable in the thermal management of steel.
Dynamic Evolution and Quantitative Characterization of Fractures in Coal at the Eastern Edge of Ordos Basin under Axial Loading
Authors: Zhu Yinghao , Tan Yineng , Zhang Songhang , Wang Mengdie , Wang Bingyi
Published: May 2023

Understanding the evolution of pore-fracture networks in coal during loading is of paramount importance for coalbed methane exploration. To shed light on these dynamic changes, this study undertook uniaxial compression experiments on coal samples collected from the eastern edge of the Ordos Basin, complemented by μ-CT scanning to obtain a 3D visualization of the crack network model. The compression process was divided into three stages, namely, micro-crack compaction, linear elasticity, and peak failure. An increase in stress resulted in greater concentration and unevenness in fractal dimensions, illustrating the propagation of initial cleats and micro-cracks in the dominant crack direction and the ensuing process of crack merging. These results provide valuable insights into the internal structure and behavior of coal under stress, informing more efficient strategies for coalbed methane extraction.