bluuebunny/crossref_metadata_2025 huggingface dataset:
Attenuating Neuronal Autophagy Alleviates Inflammatory Injury in OGD-Deprived Co-culture of HT22 with BV2
Authors: Huang Zhiwen , Liu Yuyuan , Chen Xuemei , Yu Chunlei , He Hongyun , Deng Yihao
Published: October 2023

Neuronal CX3CL1 suppressed microglial inflammation by binding to its receptor CX3CR1 expressed on microglia. Neuronal autophagy was prominently activated by cerebral ischemia, whereas CX3CL1 expression in autophagic neurons was conversely down-regulated to exacerbate microglial inflammation. Accordingly, this study was meant to investigate whether ischemia-activated microglial inflammation could be repressed by promoting CX3CL1 expression via the attenuation of neuronal autophagy. Immunofluorescence showed that autophagy predominantly occurred in neurons but barely in microglia. Western blot and immunofluorescence demonstrated that attenuating HT22 autophagy significantly increased its CX3CL1 expression and subsequently mitigated the BV2-mediated inflammatory responses, as indicated by decreased inflammatory factors of NF-B-p65, IL-6, IL-1, TNF-, and PGE2. Meanwhile, CCK-8, Nissl staining, and FJC staining showed that an OGD (Oxygen-glycogen deprivation)-created neuronal injury was greatly alleviated by CX3CL1-suppressed microglial inflammation. Contrarily, elevating HT22 autophagy markedly decreased its CX3CL1 expression, which consequently worsened microglial inflammation and the neuronal injury. Our data suggests that attenuating neuronal autophagy may be an effective method to alleviate a microglial inflammatory injury after an ischemic stroke.
The Rurikids: The First Experience of Reconstructing the Genetic Portrait of the Ruling Family of Medieval Rus’ Based on Paleogenomic Data
Authors: Zhur Kristina V. , Sharko Fedor S. , Sedov Vkadimir V. , Dobrovolskaya Maria V. , Volkov Vladimir G. , Maximov Nikita G. , Seslavin Andrey N. , Makarov Nikolay A. , Prokhortchouk Egor B.
Published: October 2023

The Rurikids were the reigning house of Rus, its principalities and, ultimately the Tsardom of Russia, for seven centuries: from the IX to the end of the XVI century. According to the Primary Chronicle (the Tale of Bygone Years), the main chronicle of Rus, the Rurik dynasty was founded by the Varangian prince Rurik, invited to reign in Novgorod in 862, but still there is no direct genetic evidence of the origin of the early Rurikids. This research, for the first time, provides a genome-wide paleogenetic analysis of bone remains belonging to one of the Rurikids, Prince Dmitry Alexandrovich (?1294), the son of the Grand Prince of Vladimir Alexander Yaroslavich Nevsky (12211263). It has been established that his Y chromosome belongs to the N1a haplogroup. Most of the modern Rurikids, according to their genealogies, belonging to the N1a haplogroup, have the most similar variants of Y chromosomes to each other, as well as to the Y chromosome of Prince Dmitry Alexandrovich. Genome-wide data of the medieval and modern Rurikids unequivocally indicates that they belong to the N1a haplogroup of the Y chromosome, starting at least from the XI century (since the time of Prince Yaroslav the Wise). All the other alleged Rurikids, both ancient and modern, being carriers of other haplogroups (R1a, I2a), possess high heterogeneity of the sequence of Y chromosomes, meaning that we cannot confirm their common ancestry. The most probable ancestors of Prince Dmitry Alexandrovich in the male line were the men who left the burial ground Bolshoy Oleny Island on the coast of the Kola Peninsula about 3,600 years ago. The reconstruction of the genome of Prince Dmitry Alexandrovich indicates the contribution of three ancestral components to his origin: (1) the early medieval population of the east of Scandinavia from the island of Oland, (2) representatives of the steppe nomadic peoples of the Eurasian steppes of the Iron Age or the early medieval population of central Europe (steppe nomads from the territory of Hungary), and (3) the ancient East-Eurasian component. Reliable statistics were also obtained when the Scandinavians were replaced with the Medieval Russian Slavic populations of the XI century. Thus, for the first time, we have shown the complex nature of interethnic interactions in the formation of the nobility of medieval Rus on the example of the ancient Rurikid.
The Role of Autophagy in the Development of Pathological Conditions of the Body
Authors: Kench Ulash S. , Sologova Susanna S. , Prasolov Vladimir S. , Spirin Pavel V.
Published: October 2023

Autophagy is the process of lysosomal elimination of the cell organelles, cytoplasmic sites, and pathogenic microorganisms that enter the cell. This process is associated with both cell death regulation and an increase in cell survival chances. Autophagy is involved in the development of various diseases (Crohn disease, cancer, atherosclerosis, etc.). For these reasons, it is of significant interest to establish the molecular targets involved in autophagy regulation and the factors that mediate its participation in pathogenesis. The review describes the potential molecular mechanisms involved in the regulation of autophagy, its contribution to the vital cell activity in a healthy organism, and pathologies.
Policy Forum : Contraintes à l'autonomie fiscale des provinces découlant des Accords de perception fiscale
Authors: Gagné-Dubé Tommy
Published: October 2023

Depuis la fin de la Seconde Guerre mondiale, les accords successifs qui ont visé à assurer une meilleure coordination fiscale entre le Canada et les provinces en matière d'imposition du revenu ont eu pour effet d'augmenter l'autonomie fiscale des provinces. La mouture actuelle des Accords de perception fiscale (APF) offre une grande latitude aux provinces dans la conception de leurs politiques fiscales, bien qu'ils contiennent inévitablement des contraintes à l'autonomie fiscale provinciale. Ce sont ces contraintes qui sont examinées, notamment à l'aide de documents obtenus dans le cadre d'une demande d'accès à l'information. Sous l'angle des modalités de perception et d'administration par le gouvernement fédéral, les contraintes ne semblent pas insurmontables et, sur certains aspects, on note même une volonté du gouvernement fédéral de les amenuiser. Sous l'angle de la nécessité pour les provinces de préserver la similitude entre le régime provincial et le régime fédéral d'impôt sur le revenu, il n'apparaît pas y avoir de marge de manœuvre en ce qui concerne la base d'imposition commune, mais l'encadrement des modifications fédérales à cette même base mériterait d'être explorée. [Agrave] la lumière des contraintes examinées et de leurs solutions potentielles ainsi que de la tâche colossale que constitue la création d'une administration fiscale propre à la province, il apparaît difficilement justifiable de s'en retirer pour une province partie à ces accords.
Differences in Results of Examination of Urine Protein Levels Dipstik Method with 6% Acetic Acid Method in Patients with Diabetes Mellitus in Area Puskesmas Baturraden II
Authors: Rahayu Feni Putri , Sulistiyowati Retno , , Sudarsono Tantri Analisawati , Rahaju Minto
Published: October 2023

Diabetes Mellitus (DM) is a non-communicable disease and a major public health issue in Indonesia and around the world. Proteinuria caused by diabetes in the blood vessels can cause kidney damage. Diabetes can also cause nerve damage in the body. This can make urinating difficult. The pressure caused by a full bladder can harm the kidneys. If urine remains in the bladder for a longer duration, it can cause an infection due to the rapid growth of bacteria in urine with a high sugar level, even if the presence of protein in the urine has been tested in a laboratory. Objective: The study aimed to determine the difference in the results of urine protein examination with the dipstick method with the 6% acetic acid method in DM patients in the working area of Puskesmas (Public Health Center) Baturraden II. Methods: The research design used was analytic observational with a cross-sectional study. Thirty urine samples were used with purposive sampling as the sampling technique. Chi-Square Test was employed for Statistical Analysis. The results of the tests produced a significant value of 0.000 (P 0,0 5), indicating that the p-value was lower in relation to the degree of error. It could be concluded that the results of urine protein examination using the dipstick method and the 6% acetic acid method differed in DM patients.
Correlation of Leukocyte Esterase Positivity Levels Against the Results of Several Examination Parameters Laboratory Urinary Tract Infection in Patients at Mataram City Regional Hospital
Authors: Waisnawa I Made Wahyu , Wiadnya Ida Bagus Rai , Manu Thomas Tandi , Agrijanti Agrijanti
Published: October 2023

Background : Urinary tract infection (UTI) is caused by the multiplication of bacteria in the urinary tract. The standard proving procedures for UTI are urine culture examination and urine dipstick examination (leukocyte esterase and nitrite). Objective : This study aims to measure leukocyte and nitrite values, calculate the total number of bacteria, identify Gram type, species and antibiotic sensitivity and analyze the correlation of leukocyte esterase positivity to the results of several UTI examination parameters. Research Methods : The method used is an analytic observational design with cross sectional design using primary data and analyzed with the Spearman rank test. Results : The results showed that in 198 patients with suspected UTI, 123 (62%) were positive for leukocyte esterase, 16 (8.1%) were positive for nitrite. The total number of bacterial countshowed 82 people (41%) with bacteriuria ≥10 5 CFU/mL. Found 61 Gram-negative bacilli and 21 Gram-positive cocci with 21 different bacterial species. Antibiotic sensitivity test showed 51% sensitive, 6% intermediate and 43% resistant. Correlation of leukocyte esterase with nitrites (p 0.023<0.05), total bacteria count (p 0.00<0.05), Gram negative bacilli (p 0.00<0.05), and Gram positive cocci (p 0.533>0.05), antibiotic sensitivity test (p 0.794 >0.05) and bacterial species (p 0.316>0.05). Conclusion : The results showed that there was a correlation between the level of positivity of leukocyte esterase and nitrite, total bacteria count and Gram-negative bacilli, but there was no correlation between Gram-positive cocci, bacterial species and antibiotic sensitivity tests.
The Relationship Between Emotional Eating And Nutritional Status In Women Of Reproductive Age In The Rimau Islands, Sumur Village
Authors: Wijaya Sofyan Musyabiq , Komala Ramadhana , Sutarto Sutarto , Nasution Syahrul Hamidi
Published: October 2023

Background: The group of women of childbearing age is one of the phases to prepare a quality next generation. Inadequate food intake can cause problems with Chronic Energy Deficiency (KEK) in women of childbearing age. In women, the presence of depressive symptoms worsens the relationship between emotional eating and food intake. This study aimed to determine the relationship between emotional eating and the level of nutritional adequacy in women of childbearing age in the Rimau Islands, Sumur Village, South Lampung.Research Methods: This study used a cross-sectional or cross-sectional design. Researchers used the Dutch Eating Behavior Questionnaire (DEBQ), translated into Indonesian, specifically for Emotional Eating The nutritional status of young women can be seen from the Body Mass Index (BMI).Research Result: This study included 20 of the 30 respondents who attended. The statistical test for emotional eating on nutritional status (obesity) has no relationship with the p-value (0.766).Conclusion: This study concludes that there is no relationship between emotional eating and Nutritional Status.
TÜRKİYE’NİN ARI TURİZMİNDE REKABET EDEBİLİRLİĞİ
Authors: UZUN Büşra , YILMAZ Emine , ÇEKEN Hüseyin
Published: October 2023

Yirmi birinci yüzyıl toplum duyarlılığının ve ihtiyaçlarının giderek değiştiği bir dönemdir. Dolayısıyla turizm sektöründe günden güne çeşitli talepler ve eğilimlerin meydana gelmesi söz konusudur. Günümüzde turizm sektöründe ön plana çıkan eğilimlerden birini bireylerin hayatını zenginleştiren doğa, kültür ve toplumla bağını güçlendiren, sürdürülebilirlik ilkeleri ile uyumlu, kırsal alanlarla doğrudan bağlantılı ekolojik turizm faaliyetleri oluşturmaktadır. Bu bağlamda dünyanın birçok yerinde gün geçtikçe yaygınlaşmaya başlayan ve arıcılıkla doğrudan bağlantılı olan arı turizmi, doğa temelli bir turizm olarak karşımıza çıkmakta ve yerel kaynakların turistik çekicilik unsuru olarak pazarlanmasında etken olduğu için rekabet edebilirlikte ülkelere ve özellikle de kırsal alanlara avantaj sağlamaktadır. Günümüzde arı turizmi modern dünyada en popüler ve en hızlı büyüyen turizm türlerinden birisidir. Bu turizm faaliyetinde turistler arı ve arıcılık ürünlerinin imalatına doğrudan katılım sağlayabilmekte; bal üretimi, tadımı ve sağımını şahsen gerçekleştirebilmektedir. Dünya ve Türkiye’de gelişmiş ve gelişmekte olan arıcılık faaliyeti tarım, turizm ve sağlık sektörü gibi birçok sektör üzerinde doğrudan ve dolaylı olarak katma değer yaratmaktadır. Dolayısıyla arı turizmi kırsal alanların kalkınmasında ve rekabet edebilirliğinde önemli bir faaliyet olarak karşımıza çıkmaktadır. Bu çalışmada nitel araştırma yöntemi kullanılmış olup birincil verilere dayalı doküman analizi ve kaynak taraması yapılmıştır. Çalışmada kaynak taramasına bağlı olarak, dünyada arı turizminde başarılı olan turizm destinasyonlarına değinilmiş, Türkiye’nin arı turizmi alanında bu destinasyonlar ile rekabet edebilirliği tartışılmış ve başarı için gerekli öneriler sunulmuştur. Bu kapsamda bu çalışmanın uygulamaya katkı sağlaması amaçlanmaktadır. Ülkemizde arıcılık faaliyetinin turizm kapsamında gerçekleştirilmesi ile turistlerin arıcılık faaliyetinin bir parçası haline gelmesinin sağlanması turizm faaliyetinde özel bir deneyim oluşturulmasına imkân verecektir. Dolayısıyla bu durum özellikle kırsal alanlarda turistik talebin daha dengeli bir hale gelmesini sağlayacak ve rekabet edebilirlikte avantaj elde edilmesine imkân verecektir.
BALIKESİR’İN GASTRONOMİK MARKA KİMLİĞİNE YENİ BİR UNSURUN KAZANDIRILMASI; TÜM YÖNLERİYLE “BALIKESİR KAHVALTISI” KONSEPTİ
Authors: ŞAHİN Candan , DOĞDUBAY Murat
Published: October 2023

Ülkelerin gelişmesinde ve sosyo-ekonomik kalkınmasında önemli rol oynayan turizm sektöründen daha fazla pay alabilmek için şehirler arasında doğan rekabet ortamı, destinasyonların markalaşması gerekliliğini ortaya koymuştur. Son yıllarda destinasyonların seçiminde önemli bir rol oynayan gastronomi turizmi, rekabet gücünün artırılmasında büyük rol oynamaktadır. Ancak her geçen gün değişen tüketici tercihleri, bu alandaki beklentileri de değiştirmekte ve çıtayı her geçen gün yükseltmektedir. Türk mutfağında önemli yeri olan kahvaltının, Balıkesir için gastronomik unsur olarak kullanılması ise hem gastronomi turizmi açısından farklılaşmaya hem de bu ürünleri üreten işletmelerin yaşanan hareketlilikten olumlu olarak faydalanmasına dolayısıyla ekonomik kalkınmaya katkı sağlayacaktır.
Çalışma kapsamında, tarımsal üretimde ve turistik çekicilikte önemli merkezlerden olan, kahvaltıda sunulan her türlü ürünü yüksek kalite ve miktarda üreten, zeytin ve peyniri ile ünlü Balıkesir’in gastronomi yönünden güçlü şehirlerle olan rekabetinde fark yaratabilmesi, destinasyon çekiciliğine yeni bir soluk kazandırılması amacıyla geliştirilen “Balıkesir Kahvaltısı” konseptinin geliştirilme nedeni, konseptin ortaya konulması aşamasında dikkate alınan hususlar, uygulama aşamasına yönelik gözlem ve deneyimler, tanıtım amaçlı yürütülen faaliyetler ile konseptin kalıcı olması konusunda yürütülmesi önerilen faaliyetlere değinilmiştir.
Çalışmanın Balıkesir gastronomisine farklı bir bakış açısı da kazandıracağı düşünülmektedir.
YÖRESEL CITTASLOW ŞEHİR LEZZETLERİNİN GASTRONOMİ TURİZMİ AÇISINDAN ÖNEMİ: KUZEY KIBRIS ÖRNEĞİ
Authors: ÇAVUŞOĞLU Mehmet
Published: October 2023

Akdeniz’in İncisi olarak bilinen Kıbrıs adası, binlerce yıllık insanlık tarihi geçmişi, arkeolojik, gastronomik, turistik ve doğal çevre zenginliği ile hem kitle turizmi hem de bir alternatif turizm çeşidi olan gastronomi turizmi faaliyetleri ile öne çıkan bir turistik destinasyondur. Dünya gastronomi turizm çalışmalarında destinasyonların çekiciliğini artıran, turizm gelirlerinin artmasını sağlayan, dünya çapında tanınırlığına destek olan, yöresel gastronomiye önem veren ve yerel yiyecek içecek kültürünün sürdürülebilirliğine destek sağlayan Cittaslow hareketine üyelik konusudur. Bu çalışmada öncelikle Kuzey Kıbrıs’taki Cittaslow unvanlı şehirlerde mevcut olan ve gastronomi turizmi çalışmalarında kullanılabilecek yöresel lezzetleri tespit etmek, daha sonra araştırmadan elde edilen bulguları kullanarak Cittaslow şehirlerde yapılan gastronomi turizmi faaliyetlerinde kullanılmak üzere gerekli politika ve stratejiler geliştirilmek hedeflenmiştir. Çalışmanın ilk bölümünde gastronomi, gastronomi turizmi kavramı, yöresel yiyecek içecek kavramı ve gastronomi turizmi açısından önemi, Kuzey Kıbrıs yiyecek içecek kültürü ile Cittaslow Kuzey Kıbrıs konuları açıklanmıştır. Çalışmanın araştırma bölümünde Nitel bir araştırma çeşidi olan doküman analizi yöntemi ile yapılmış olan bir çalışma yer almaktadır. Çalışma kapsamında yapılmış olan araştırma evrenini Kuzey Kıbrıs’taki Cittaslow hareketine üye şehirler oluşturmaktadır. Araştırma sonucunda, yapım, sunum ve lezzet özellikleri bakımından önemli çekicilik potansiyeline sahip 63 adet yöresel lezzet tespit edilmiştir. Çalışmanın sonuç bölümünde, yöresel lezzetlerin, Cittaslow şehirler açısından önemi açıklanmıştır. Öneriler bölümünde ise Kuzey Kıbrıs’taki Cittaslow şehirlerde mevcut olan yöresel lezzetlerin sürdürülebilirliğinin sağlanması ve dünya çapında tanıtılması için yapılması gerekenler hakkında öneriler yer almaktadır.
Assessment of the Cytotoxic Effects of Dimethyl Sulfoxide (DMSO) on MCF-7 and HeLa Cell Line
Authors: Suharyani Suharyani , Danan Jaya Bayu Putra
Published: October 2023

Cell culture studies on drugs and natural compounds are commonly employed for early compound screening, assessing their potential therapeutic and immunomodulatory effects in vitro. Many of these compounds have limited solubility in water and are typically dissolved in lipophilic solvents. Consequently, it is crucial to employ a biocompatible and non-toxic solvent for cell viability. Dimethyl sulfoxide (DMSO) is a widely utilized solvent in in vitro testing. This study aims to investigate the effect of DMSO on the viability of HeLa and MCF-7 cell lines. The MTT method was employed to assess cytotoxic effects on the HeLa and MCF-7 cell lines. The results revealed that concentrations of 1% and 2% DMSO significantly reduced the viability of MCF-7 cells (p<0.05) compared to the control after 24 hours of incubation. Conversely, the viability of HeLa cell lines exposed to 1% and 2% DMSO concentrations did not significantly differ from the control after 24 hours. Thus, concentrations of 1% and 2% DMSO exhibited cytotoxicity towards MCF-7 cells, while no such effect was observed in HeLa cells.
PENGARUH PEMBERIAN COOKIES DENGAN PENAMBAHAN TEPUNG TEMPE DAN KACANG MERAH TERHADAP PENINGKATAN BERAT BADAN BALITA GIZI KURANG
Authors: Aprilia Aprilia , Sartono Sartono , Siregar Afriyana
Published: October 2023

Masalah kekurangan gizi pada balita masih cukup tinggi, berdasarkan hasil SSGI 2021, masalah gizi kurang di Indonesia untuk kelompok umur 0-59 bulan sebesar 17% sedangkan untuk prevalensi balita gizi kurang di Sumatera Selatan sebesar 15,7% dan untuk prevalensi balita gizi kurang di kota Palembang sebesar 16,1%.
Tujuan penelitian adalah diketahui pengaruh pemberian cookies dengan penambahan tepung tempe dan kacang merah terhadap peningkatan berat badan balita gizi kurang di Wilayah Kerja Puskesmas Taman Bacaan Palembang. Metode penelitian ini adalah Quasi Experiment (Ekskperimen semu) dengan desain penelitian pretest posttest control group design. Uji statistik yang digunakan adalah uji t-dependent dan uji t-independent yang dianalisis secara univariat dan bivariat. Hasil penelitian menunjukkan Rata-rata berat badan sampel sebelum intervensi pada kelompok perlakuan yaitu 8,88 kg menjadi 9,33kg setelah intervensi. Sedangkan pada kelompok pembanding yaitu 9,66 kg menjadi 9,95 kg setelah intervensi. Hasil uji statistik (t-independent) di dapatkan p value 0,006 dapat disimpulkan bahwa ada pengaruh pemberian cookies dengan penambahanan tepung tempe dan kacang merah terhadap peningkatan berat badan balita gizi kurang di wilayah kerja Puskesmas Taman Bacaan Palembang. Disarankan kepada orang tua balita dapat melanjutkan konsumsi makanan yang  tinggi kalori seperti formula cookies dengan penambahan  tepung tempe dan  kacang merah, sehingga dapat menaikkan berat badan balita secara bertahap.
 
Kata Kunci: gizi kurang, berat badan, cookies dengan penambahan tepung tempe dan kacang merah
Pola Konsumsi Makanan Sumber Asam Lemak Trans pada mahasiswa PTS Kota Bengkulu di Era Pandemi Covid 19
Authors: Halimah Halimah , Elly Nur
Published: October 2023

Obesitas merupakan factor resiko berkembangnya Penyakit Tidak Menular (PTM). Obesitas terjadi karena penumpukan lemak akibat tidak seimbangnya antara asupan dan pengeluaran energi. Perubahan gaya hidup remaja yang cenderung lebih memilih makanan siap saji dan terbatasnya aktifitas beresiko terhadap obesitas. Masa  berkembangnya Covid 19  melahirkan kebijakan adanya pembatasan aktifitas dan upaya memperbaiki asupan nutrisi untuk meminimalisir resiko penularan. Penelitian ini bertujuan untuk mengetahui pola konsumsi makanan sumber asam lemak trans pada mahasiswa Perguruan Tinggi Swasta Kota Bengkulu di era pandemi Covid 19.
Metode penelitian: Studi ini merupakan metode survey sebagai studi awal untuk melihat pola konsumsi makanan sumber asam lemak trans pada mahasiswa baru perguruan tinggi swasta kota Bengkulu di era pandemi Covid 19. Sampel berjumlah 40 mahasiswa dengan metoda purposive sampling. Pola konsumsi makanan sumber asam lemak trans menggunakan food frequency questionnaires (FFQs). Penyebaran kuisioner dibagikan secara online melalui Google form. Data diolah dan dianalisis secara deskriptif.  Hasil: proporsi frekuensi konsumsi tinggi pada makanan susu dan olahannya serta ice cream dan minumannya. Makan gorengan merupakan makanan dengan proporsi frekuansi tinggi pada makanan jenis tahu dan tempe goreng, telur goreng, bakwan, ikan dan ayam goreng. Proporsi frekuensi tinggi produk HVO yang dikonsumsi adalah roti, biskuit, wafer.
Kesimpulan: produk olahan susu dan ice cream serta makanan gorengan memiliki proporsi frekuensi  konsumsi yang tinggi. Konsumsi sayur >3x/hari pada sayuran hijau. Penelitian selanjutnya lebih dikembangkan pada hubungan pola konsumsi dengan status gizi remaja serta profil lipid.
Kata kunci: frekuensi konsumsi, asam lemak trans, remaja
Peningkatan Pengetahuan Mengenai Dampak Cyberbullying Terhadap Kesehatan Mental Pada Remaja
Authors: Septianawati Paramita , Finurina Mustikawati Irma , Ratna Kusuma Inggar , Pratama Tisna Sendy , Paramita Hilma
Published: October 2023

Masyarakat hampir sebagian besar memiliki alat penghubung dengan internet antara lain handphone, laptop, tablet dan lain sebagainya. Penggunaan alat tersebut berfungsi sebagai alat telekomunikasi yang dapat dipakai secara positif atau pun negatif oleh masing-masing individu pengguna. Penggunaan negatif media internet dapat berupa penyebaran isu atau berupa penindasan melalui media sosial yang dikenal cyberbullying. Penindasan melalui media elektronik dapat menimbulkan masalah kesehatan masyarakat terutama remaja berupa rasa cemas, stres, percaya diri rendah dan lain sebagainya. Dampak yang lebih parah lagi dapat mengakibatkan anak muda atau remaja yang terkena aksi cyberbullying akan mendapat kemampuan yang rendah di bidang akademik dan dapat terjadi peningkatan kejadian bolos sekolah; atau terjadinya risiko bunuh diri. Maka dari itu tujuan program pengabdian masyarakat yang ditawarkan adalah pemberian Sosialisasi Dampak cyberbullying terhadap Kesehatan Mental. Kegiatan dilakukan dimulai dari pemeriksaan glukosa darah, pretes, kemudian penyuluhan mengenai bahayanya cyberbullying dapat menyebabkan terjadinya gangguan mental, dilanjutkan dengan postes, serta membuat poster berupa stop cyberbullying. Hasil pengabdian mengenai tingkat pengetahuan ditunjukkan adanya peningkatan rerata nilai hasil pengetahuan, dimana sebelum diberikan materi di dapatkan rerata adalah 75,22; kemudian setelah diberikan materi rerata nilai postes adalah 87,78, artinya terdapat penambahan pengetahuan mengenai materi dampak cyberbullying terhadap kesehatan mental pada remaja di Panti Asuhan Putri Muhammadiyah Tanjung, Purwokerto. Kesimpulan pada kegiatan pengabdian adalah adanya efektivitas pemberian materi dan pembuatan poster stop cyberbullying terhadap peningkatan pengetahuan pada remaja putri mengenai dampak cyberbullying terhadap kesehatan mental.
Redes de conocimiento en relación a agendas endógenas como alternativa a la tensión internacional-local
Authors: Oregioni María Soledad , Avondet Laura , Durán María Sol
Published: October 2023

La ciencia y la tecnología han ganado relevancia en las agendas en la medida en que se las reconoce como componentes imprescindibles de cualquier estrategia que pretenda hacer frente a “desafíos actuales”, muchos de los cuales son definidos como tales por los países del Norte. Sin embargo, desde el Sur entendemos que los conocimientos no son neutrales y cuestionamos el concepto de desarrollo en la medida en que, a través de su concepción tradicional, no hemos logrado dar respuesta a los problemas más acuciantes de la región. El presente trabajo muestra cómo, a partir de la identificación de una problemática concreta -la producción de cannabis medicinal- fue posible generar redes formales e informales, integradas por diferentes actores (territoriales e internacionales) en las que se habilitaron nuevas dinámicas de producción de conocimiento a partir de la convergencia de diferentes tipos de saberes y actores. Específicamente, se analizó como caso de estudio la producción de conocimiento sobre cannabis medicinal en la Universidad Nacional del Centro de la Provincia de Buenos Aires (UNICEN).
La figura legal del femicidio y la violencia de género en el Ecuador
Authors: Idrovo Heredia Luis Miguel , Zamora Vázquez Ana Fabiola
Published: October 2023

Introducción: Actualmente las estadísticas dan cuenta de la intensidad del conflicto de género en el país, reflejado en diversas formas de violencia hacia la mujer. Por lo tanto, el delito de femicidio ha sido tipificado en las legislaciones de catorce Estados de Latinoamérica; esto se encuentra explicado en la implementación de una estrategia política-conceptual, la cual se ha configurado desde las perspectivas feministas buscando que la sociedad sea consciente de la problemática de muertes violentas a causa del género; buscando reafirmar los compromisos de los Estados. Objetivos: Establecer que la tipificación del femicidio no ha logrado disminuir la cifra de muertes violentas de mujeres en el país, y se evidencia un incremento del femicidio. Metodología: Dentro de este artículo se realiza una fundamentación teórica sobre los antecedentes jurídicos y sociales que motivaron el surgimiento de la tipificación autónoma del femicidio en el Ecuador, distinguiendo el impacto que ha tenido la tipificación de femicidio en los niveles de muertes violentas a mujeres en el Ecuador. Resultados: Dentro de la problemática de género en el país, se ha reportado que, las sanciones para la violencia doméstica no reflejan la gravedad de las actuaciones de los agresores, los cuales habitualmente presentan una conducta determinada por una violencia intensificada a medida que la víctima lo permite y el tiempo de agresión; demandando de forma urgente la activación del aparataje y los recursos estatales que permitan que los derechos de las mujeres, se garantice de acuerdo a como se expresa en la normativa. Conclusiones: La tipificación del femicidio en el Ecuador no ha logrado disminuir la cifra de muertes violentas de mujeres en el país, y se evidencia un incremento del femicidio en el territorio patrio Área de estudio general: Derecho. Área de estudio específica: Derecho procesal.
Principles of transport means maintenance optimization: equipment cost calculation
Authors: Golovan A , Gritsuk I , Honcharuk I
Published: October 2023

Purpose. Justification of principles and methodology for effective calculation of the equipment costs and optimization of transport means maintenance. Methodology. The results of the presented scientific research were obtained using general and special methods of cognition: abstract and logical analysis, systematization and combination, method of theoretical generalization, method of dialectical cognition, deduction and induction, and statistical analysis. This paper analyzes the relationship between the probability of failure prevention by the maintenance system and the associated costs. The research investigates how the variation in the technical condition change rate influences the length of the operation cycle and the rate of its decline. The study’s outcomes are analyzed, including the formation of points of minimum unit costs, the effect of spare parts’ cost, and the practical importance of the conclusions drawn. Findings. This paper outlines the economic methodology for determining the specific expenses of maintaining means of transport. The methodology considers the distribution of expenses for spare parts, labor, and other components. Using this methodology, it is possible to estimate the total costs of maintenance and make informed decisions about the efficient use of resources. It has been determined that the cost of spare parts impacts the efficiency of the maintenance system. Therefore, it is imperative to balance the cost for spare parts and safety, while considering the probability of failure. The method outlined in this work is versatile, which allows its adaptation and application to the specialized road transport. Originality. The paper further develops the methodological approach to calculating equipment costs for transport maintenance, which is used to improve service efficiency and reduce expenses. The approach enables a comprehensive evaluation of the outcomes of enhancing failure prevention probability through the maintenance system. It also aids in managing unused parts’ resources, particularly during short operating cycles. Practical value. The study’s findings can optimize the maintenance system, increase operational efficiency, and enhance the safety and reliability of means of transport, while reducing the costs associated with spare parts, labor, and other maintenance components. This approach aids in conserving resources, reducing operating costs, and is crucial for the financial stability and profitability of management companies.
The Relationship between Transcranial Doppler Ultrasonography and Visual Field Test Results in Glaucoma and Glaucoma Suspect Patients
Authors: Han Hyo Ji , Kim Joon Mo
Published: October 2023

Purpose: To evaluate the relationships between parameters of transcranial ultrasonography and results of visual field tests in patients with open angle glaucoma or suspected of having glaucoma.Methods: This retrospective study was based on data from medical records of patients who visited the Department of Ophthalmology in Kangbuk Samsung Hospital from January 1, 2016, to October 17, 2019, and underwent transcranial Doppler ultrasonography as part of a routine health examination. Ophthalmic data were visual acuity, intraocular pressure, optical coherence tomography, and Humphrey visual field test results. Retinal nerve fiber layer defect was confirmed by a glaucoma specialist. Patients’ ophthalmic data, such as average ganglion cell layer thickness, visual field index, pattern standard deviation, and mean deviation, were divided into quartiles. Each ophthalmic artery parameter from transcranial Doppler ultrasonography was compared between quartiles.Results: A total of 162 patients were reviewed. There was no difference in Doppler ophthalmic artery (OA) parameters between patients with or without retinal nerve fiber layer defect. None of the quartile groups of average ganglion cell layer thickness showed significant difference in any OA parameters. Patients in the low–visual field index quartile showed significant low peak systolic velocities of OAs when adjusted for age, sex, and presence of diabetes mellitus or hypertension (p = 0.016). A higher pattern standard deviation showed lower peak systolic velocity (p = 0.046). There was no significant tendency between any other OA parameter and mean deviation value.Conclusions: Our study suggests that hemodynamic parameters of ophthalmic arteries might be associated with visual field status of patients. Further large-population studies are needed in order to better understand the relationship between visual function and ocular blood flow.
A Comprehensive Analysis of the Influence of Temperature and Humidity on Dry Eye Disease
Authors: Song Myung-Sun , Lee Yunjin , Paik Hae Jung , Kim Dong Hyun
Published: October 2023

Purpose: To investigate the effects of humidity and temperature on dry eye disease (DED)Methods: A retrospective, clinic-based study was conducted on DED patients undergoing dry eye treatment. Patients were followed up at least twice, and symptoms and signs were evaluated using the Symptoms Assessment Questionnaire in Dry Eye (SANDE) score, tear secretion, tear film breakup time (TBUT), ocular staining score, and tear osmolarity. Mean humidity and temperature values for 1 week before ocular examinations were used as the environmental exposure level. The relationship between humidity and temperature, with DED clinical parameters was analyzed in single- and multi-environmental factor models.Results: The study included 33 patients with a mean age of 53.9 ± 12.2 years. The low humidity group showed significantly higher SANDE scores (p = 0.023) and tear osmolarity (p = 0.008), and the low temperature group had higher SANDE scores (p = 0.004), ocular staining scores (p = 0.036), and tear osmolarity (p < 0.001). In the linear mixed model, single factor analysis showed that an increase in humidity resulted in decreased SANDE scores (p = 0.043), and an increase in temperature led to a decrease in SANDE score (p = 0.007), ocular staining score (p = 0.007), and tear osmolarity (p = 0.012). In the multifactor analysis, changes in humidity had no significant effect on dry eye parameters, but an increase in temperature was significantly correlated with decreased SANDE score (p = 0.026), ocular staining score (p = 0.024), and tear osmolarity (p = 0.002).Conclusions: Lower temperature led to aggravated symptoms and signs of DED and the effect of temperature on DED was more pronounced than humidity. Tear osmolarity was the most sensitive clinical parameter to be affected by climate factors in DED patients.
The EU’s New Energy and Climate Policy: Energy Security and the Moderation of Environmental Standards
Authors: Olivares Alberto
Published: October 2023

The article analyzes the evolution of the EU's energy and climate policy to explain that since Russia invaded Ukraine, a reformulation has occurred, characterized by the reconciliation of climate objectives with energy security and the flexibility of the environmental dimension. This rearrangement of the objectives of environmental policies has begun to take shape with the REPowerUE Plan, an energy package with which the EU intends to respond to the two dimensions of Europe's energy crisis: the need to deepen the transition towards climate neutrality and the duty to abandon dependence on Russian hydrocarbons. The review is based on two specific measures: Commission Delegated Regulation (EU) 2022/1214 and Council Regulation EU 2022/2577, key regulations for the massive deployment of renewable energies.
The Climate Emergency: Putting Consumers at the Heart of the European Green Deal Following Russia's War on Ukraine
Authors: Encinar Arroyo Nuria
Published: October 2023

On 24 February 2022, Russia launched a military invasion on Ukraine that created a humanitarian catastrophe by destroying lives, homes and infrastructure. The illegal invasion has had significant consequences: electricity prices have soared and wholesale gas prices have more than doubled, sparking a global energy crisis. Another consequence has been global food prices rising to the highest levels ever recorded. On the other hand, consumers have to be at the core of the EU’s energy policy. For this to happen, a wide range of initiatives have to be implemented to make consumers an active part of the clean energy transition and help them save money and energy. To this end, this work will first review the liberalization process, and then discuss how green liberalization can be advanced in light of the Green Deal. Following this, we will explore how consumers will engage in future energy markets and then we will move on to how the war affects consumers and Europe’s ability to reach carbon neutrality in the second half of the 21st century.
The Key Tenets of a Hydrogen Strategy: An Analysis and Comparison of the Hydrogen Strategies of the EU, Germany and Spain
Authors: Pinto Jaqueline
Published: October 2023

The article examines the hydrogen strategies of Spain and Germany, juxtaposing each with one another under the umbrella of the EU Strategy. Both Spain and Germany have positioned themselves at the forefront of creating hydrogen economies. Hence, looking at their hydrogen strategies provides an opportunity to ascertain how these nations set out the plans guiding their hydrogen ambitions. The article examines the similarities and differences between the strategies to ascertain a hydrogen strategy's core requirements and provides recommendations for further revisions. Through this analysis, it can be established that a hydrogen strategy requires: (i) capitalising on local and geographic strengths and cooperation with other nations; (ii) a focus on a type of hydrogen and definitions which are uniform with those of other nations; (iii) clear timelines and targets; as well as (iv) fiscal and other measures to achieve targets. These key pillars must be set out within the broader context of a nation’s climate change targets. Hydrogen is experiencing a renewed interest, and it appears as if its time has finally arrived; these strategies show the enthusiasm of governments to ensure that hydrogen is a pivotal player in the energy landscape.
EU Critical Raw Minerals Act Highlights Intensifying Competition in Race to Net Zero
Authors: Leon Peter , Muller Ernst , Svilanovic Tihomir , Yolland Alessandra
Published: October 2023

Global competition to ensure a secure supply of critical raw materials (‘CRMs’) is driven by two principal factors: the burgeoning global demand for CRMs deemed critical to the green and digital transition, as well as the defence sector; and supply chain characteristics accentuated during the COVID-19 pandemic and exacerbated by Russia's invasion of Ukraine. The EU has entered the CRM arms race with the EU Commission's proposed CRM Act, which sets non-binding targets aimed at bolstering domestic production of strategic raw materials by 2030. As the EU is significantly reliant on third country imports of these materials, these targets are particularly ambitious, not least in light of the EU's limited industrial policy remit. Changes to permitting processes, the creation of Strategic Projects based on sustainability criteria and stronger international cooperation are key factors likely to shape the extent to which the CRM Act will enable the EU to compete for these naturally finite but critical materials.
Gendered Issues or Partisan Priorities? The Policy Priorities of Men and Women at Partisan Think Tanks
Authors: Manning Andrea , Fagan E.J.
Published: October 2023

This paper examines the relationship between gender, partisanship, and authorship of partisan think tank reports. Scholars often observe a gendered dimension to issues, where women are associated with issues related to child-rearing, education, and social welfare while men are associated with issues related to violent state action, such as foreign policy and crime. However, these studies are often limited by the confounding variable of partisanship, where political parties tend to prioritize certain issues. We solve these problems by analyzing the policy content and authors of partisan think tank reports in the United States. We introduce a dataset of 16,229 authors of 10,386 reports from partisan think tanks in the U.S., coded for their policy content using the Comparative Agendas Project subtopic coding system. We find that while women elites tend to prioritize issues often associated with women in both parties, prioritization of issues by sex is more complicated.
Implementasi Portabel Solar Panel sebagai Energi Penggerak Pompa Air di Desa Toniku
Authors: Hadi Rian Maulana , Rais Sandi , Tjiroso Bambang , Sirad Mochammad Apriyadi Hadi
Published: October 2023

Penelitian ini bertujuan untuk menganalisa pemakaian daya listrik pada pompa air yang dihasilkan oleh pembangkit listrik tenaga surya portable, dengan memasang pembangkit listrik tenaga surya portable sebagai alat uji dan suplai energi listrik pada pompa air tersebut. Metode yang digunakan dalam penelitian ini adalah metode kualitatif dan experiment, dimana dilakukan survei dan wawancara langsung ke masyarakat yang terdampak khusunya masyarakat Desa Toniku, kemudian melakukan advokasi data-data selanjutnya dilaksanakannya pemasangan solar panel portable pada pompa air, analisa dan pengujian. Hasil dari pengujin selama 5 hari, pengujian juga dilakukan pada malam hari, efektifitas pompa bekerja hanya sampai 1,5 jam setelah dari 1,5 jam pompa tidak lagi bekerja secara maksimal sampai pada menit ke 240, pompa tersebut masih tetap menyala namun tidak menghasilkan air sampai pada menit 450. Kinerja pompa mulai dari hari pertama sampai dengan hari ketiga, pada hari ketiga kinerja pompa berada pada nilai tertinggi yakni 25,038 Joule. Untuk memaksimalkan kinerja pompa air tersebut maka dibutuhkan penambahan 1 buah baterai dengan kapasitas 100 Ah,12 Volt. Agar dapat menggerakan pompa secara maksimal, efektivitas dari sinar matahari selama 8 jam yang diperoleh dari pada perhitungan sebesar 800 Watt, oleh karena itu untuk memenuhi kebutuhan daya dari baterai pada pemakain selama 8 jam sebesar 9.600 Watt. Maka besar dari daya baterai tersebut dibagi dengan total ouput dari solar panel sebesar 800-Watt maka dibutuhkan 12 panel surya dengan masing-masing ouput daya 100 Wp.
CRISPR-based bioengineering in microalgae for production of industrially important biomolecules
Authors: Dhokane Dhananjay , Shaikh Arshi , Yadav Anu , Giri Nandinee , Bandyopadhyay Anindya , Dasgupta Santanu , Bhadra Bhaskar
Published: October 2023

Microalgae, as photosynthetic organisms, have the potential to produce biomolecules for use in food, feed, cosmetics, nutraceuticals, fuel, and other applications. Faster growth rates and higher protein and lipid content make microalgae a popular chassis for many industrial applications. However, challenges such as low productivity and high production costs have limited their commercialization. To overcome these challenges, bioengineering approaches such as genetic engineering, metabolic engineering, and synthetic biology have been employed to improve the productivity and quality of microalgae-based products. Genetic engineering employing genome editing tools like CRISPR/Cas allows precise and targeted genetic modifications. CRISPR/Cas systems are presently used to modify the genetic makeup of microalgae for enhanced production of specific biomolecules. However, these tools are yet to be explored explicitly in microalgae owing to some limitations. Despite the progress made in CRISPR-based bioengineering approaches, there is still a need for further research to optimize the production of microalgae-based products. This includes improving the efficiency of genome editing tools, understanding the regulatory mechanisms of microalgal metabolism, and optimizing growth conditions and cultivation strategies. Additionally, addressing the ethical, social, and environmental concerns associated with genetic modification of microalgae is crucial for the responsible development and commercialization of microalgae-based products. This review summarizes the advancements of CRISPR-based bioengineering for production of industrially important biomolecules and provides key considerations to use CRISPR/Cas systems in microalgae. The review will help researchers to understand the progress and to initiate genome editing experiments in microalgae.
Case Report: The impact of severe cryptosporidiosis on the gut microbiota of a pediatric patient with CD40L immunodeficiency
Authors: Piazzesi Antonia , Pane Stefania , Russo Alessandra , Del Chierico Federica , Francalanci Paola , Cotugno Nicola , Rossi Paolo , Locatelli Franco , Palma Paolo , Putignani Lorenza
Published: October 2023

Cryptosporidium parvum is a protozoan parasite and one of the leading causes of gastroenteritis in the world, primarily affecting very young children and immunocompromised patients. While infection is usually self-limiting, it can become chronic and even lethal in these vulnerable populations, in whom Cryptosporidium treatments are generally ineffective, due to their acting in concert with a functioning immune system. Here, we describe a case of chronic cryptosporidiosis in a European child with severe CD40L immunodeficiency infected with Cryptosporidium parvum of the IIa20G1 subgenotype, a lineage which has thus far only ever been described in the Middle East. After years of on-off treatment with conventional and non-conventional anti-parasitic drugs failed to clear parasitosis, we performed targeted metagenomics to observe the bacterial composition of the patient’s gut microbiota (GM), and to evaluate fecal microbiota transplantation (FMT) as a potential treatment option. We found that C. parvum infection led to significant shifts in GM bacterial composition in our patient, with consequent shifts in predicted intestinal functional signatures consistent with a state of persistent inflammation. This, combined with the patient’s poor prognosis and increasing parasitic burden despite many rounds of anti-parasitic drug treatments, made the patient a potential candidate for an experimental FMT procedure. Unfortunately, given the many comorbidities that were precipitated by the patient’s immunodeficiency and chronic C. parvum infection, FMT was postponed in favor of more urgently necessary liver and bone marrow transplants. Tragically, after the first liver transplant failed, the patient lost his life before undergoing FMT and a second liver transplant. With this case report, we present the first description of how cryptosporidiosis can shape the gut microbiota of a pediatric patient with severe immunodeficiency. Finally, we discuss how both our results and the current scientific literature suggest that GM modulations, either by probiotics or FMT, can become novel treatment options for chronic Cryptosporidium infection and its consequent complications, especially in those patients who do not respond to the currently available anti-parasitic therapies.