bluuebunny/crossref_metadata_2025 huggingface dataset:
The Influence of Surgical Staff Behavior on Air Quality in a Conventionally Ventilated Operating Theatre during a Simulated Arthroplasty: A Case Study at the University Hospital of Parma
Authors: Pasquarella Cesira , Balocco Carla , Colucci Maria Eugenia , Saccani Elisa , Paroni Samuel , Albertini Lara , Vitali Pietro , Albertini Roberto
Published: January 2020

Surgical staff behavior in operating theatres is one of the factors associated with indoor air quality and surgical site infection risk. The aim of this study was to apply an approach including microbiological, particle, and microclimate parameters during two simulated surgical hip arthroplasties to evaluate the influence of staff behavior on indoor air quality. During the first hip arthroplasty, the surgical team behaved correctly, but in the second operation, behavioral recommendations were not respected. Microbiological contamination was evaluated by active and passive methods. The air velocity, humidity, temperature, and CO2 concentration were also monitored. The highest levels of microbial and particle contamination, as well as the highest variation in the microclimate parameter, were recorded during the surgical operation where the surgical team behaved “incorrectly”. Turbulent air flow ventilation systems appeared more efficient than in the past and very low air microbial contamination was reached when behavior was correct. Therefore, adherence to behavioral recommendations in operating theatres is essential to not undermine the effectiveness of the heating, ventilation, and air conditioning systems and employed resources.
The Impact of Sprint Interval Training Frequency on Blood Glucose Control and Physical Function of Older Adults
Authors: Adamson Simon , Kavaliauskas Mykolas , Lorimer Ross , Babraj John
Published: January 2020

Exercise is a powerful tool for improving health in older adults, but the minimum frequency required is not known. This study sought to determine the effect of training frequency of sprint interval training (SIT) on health and physical function in older adults. Thirty-four (13 males and 21 females) older adults (age 65 ± 4 years) were recruited. Participants were allocated to a control group (CON n = 12) or a once- (n = 11) or twice- (n = 11) weekly sprint interval training (SIT) groups. The control group maintained daily activities; the SIT groups performed 8 weeks of once- or twice-weekly training sessions consisting of 6 s sprints. Metabolic health (oral glucose tolerance test), aerobic capacity (walk test) and physical function (get up and go test, sit to stand test) were determined before and after training. Following training, there were significant improvements in blood glucose control, physical function and aerobic capacity in both training groups compared to control, with changes larger than the smallest worthwhile change. There was a small to moderate effect for blood glucose (d = 0.43–0.80) and physical function (d = 0.43–0.69) and a trivial effect for aerobic capacity (d = 0.01) between the two training frequencies. Once a week training SIT is sufficient to produce health benefits. Therefore, the minimum time and frequency of exercise required is much lower than currently recommended.
Potentially Heterogeneous Cross-Sectional Associations of Seafood Consumption with Diabetes and Glycemia in Urban South Asia
Authors: Gribble Matthew O. , Head Jennifer R. , Prabhakaran Dorairaj , Kapoor Deksha , Garg Vandana , Mohan Deepa , Anjana Ranjit Mohan , Mohan Viswanathan , Vasudevan Sudha , Kadir M. Masood , Tandon Nikhil , Narayan K. M. Venkat , Patel Shivani A. , Jaacks Lindsay M.
Published: January 2020

Aims: In this study, we aimed to estimate cross-sectional associations of fish or shellfish consumption with diabetes and glycemia in three South Asian mega-cities. Methods: We analyzed baseline data from 2010–2011 of a cohort (n = 16,287) representing the population ≥20 years old that was neither pregnant nor on bedrest from Karachi (unweighted n = 4017), Delhi (unweighted n = 5364), and Chennai (unweighted n = 6906). Diabetes was defined as self-reported physician-diagnosed diabetes, fasting plasma glucose ≥126 mg/dL (7.0 mmol/L), or glycated hemoglobin A1c (HbA1c) ≥6.5% (48 mmol/mol). We estimated adjusted and unadjusted odds ratios for diabetes using survey estimation logistic regression for each city, and differences in glucose and HbA1c using survey estimation linear regression for each city. Adjusted models controlled for age, gender, body mass index, waist–height ratio, sedentary lifestyle, educational attainment, tobacco use, an unhealthy diet index score, income, self-reported physician diagnosis of high blood pressure, and self-reported physician diagnosis of high cholesterol. Results: The prevalence of diabetes was 26.7% (95% confidence interval: 24.8, 28.6) in Chennai, 36.7% (32.9, 40.5) in Delhi, and 24.3% (22.0, 26.6) in Karachi. Fish and shellfish were consumed more frequently in Chennai than in the other two cities. In Chennai, the adjusted odds ratio for diabetes, comparing more than weekly vs. less than weekly fish consumption, was 0.81 (0.61, 1.08); in Delhi, it was 1.18 (0.87, 1.58), and, in Karachi, it was 1.30 (0.94, 1.80). In Chennai, the adjusted odds ratio of prevalent diabetes among persons consuming shellfish more than weekly versus less than weekly was 1.08 (95% CI: 0.90, 1.30); in Delhi, it was 1.35 (0.90, 2.01), and, in Karachi, it was 1.68 (0.98, 2.86). Conclusions: Both the direction and the magnitude of association between seafood consumption and glycemia may vary by city. Further investigation into specific locally consumed seafoods and their prospective associations with incident diabetes and related pathophysiology are warranted.
Comment on Lecca, L.I.; Portoghese, I.; Mucci, N.; Galletta, M.; Meloni, F.; Pilia, I.; Marcias, G.; Fabbri, D.; Fostinelli, J.; Lucchini, R.G.; Cocco, P.; Campagna, M. Association between Work-Related Stress and QT Prolongation in Male Workers. Int. J. Environ. Res. Public Health 2019, 16, 4781
Authors: Alizargar Javad , Hsieh Nan-Chen , Wu Shu-Fang Vivienne , Weng Shih-Yen
Published: January 2020

Lecca et al., in a recent publication in the Int. J. Environ. Res. Public Health had made some mistakes in the statistical analysis and interpretation of the results. Age was not a clear contributing factor in the prolongation of QT interval in the electrocardiogram (ECG), as there were strong confounders in their study. The effects of age were mainly faded out because of the age range of the participants. The use of Pearson’s correlation is questionable because of the normality assumptions was not met on the studied variables. They also made some conclusions about the effects of long and night shifts on the QT prolongation that were not appropriate based on their study type. All of these mentioned issues might completely change the validity of the conclusions they made.
Anxiety and Depression Associated with Anal Sexual Practices among HIV-Negative Men Who Have Sex with Men in Western China
Authors: Liu Jiaxiu , Zhong Xiaoni , Lu Zhuo , Peng Bin , Zhang Yan , Liang Hao , Dai Jianghong , Zhang Juying , Huang Ailong
Published: January 2020

This study aimed to explore the prevalence and influencing factors of anxiety and depression among human immunodeficiency virus negative (HIV-negative) men who have sex with men (MSM) based on anal sex roles, so as to provide a scientific basis for the management of mental health conditions. Data were obtained from the baseline in a cohort study with a two-year follow-up period in western China. The Self-Rating Anxiety Scale and Center for Epidemiological Studies Depression Scale were used to assess anxiety and depression symptoms, respectively. The prevalence of anxiety and depression in 1771 MSM was 26.03% and 37.83%. Among them, 182 anal sex role “0” MSM who only had receptive anal sex with men reported the highest prevalence of anxiety and depression (31.32% and 46.15%), 467 anal sex role “1” MSM who only had insertive anal sex with men (22.27% and 32.76%), and 1122 anal sex role “0.5” MSM who engaged equally in both insertive and receptive anal sex intercourse with men (26.74% and 38.59%), respectively. Logistic regression analysis revealed that the influencing factors of anxiety include anal sex role “0”/”0.5”, low educational level, female sexual partners, frequently searching partners on the Internet, sexually transmitted diseases (STD) diagnosed by doctors, and no HIV counseling. Anal sex role “0”/”0.5”, rural area, casual male partners, female partners, STD diagnosed by doctors, frequently searching partners on the Internet, no HIV counseling, no condom use, and daily alcohol use were found to be associated with depression. In conclusion, early identification and intervention of anxiety and depression symptoms in HIV-negative MSM should be carried out, especially for anal sex role “0” MSM. Furthermore, key intervention and psychological counseling should be taken into consideration for MSM with a low education level, high-risk behaviors, and high-risk perceptions.
Healthcare Associated Infections: An Interoperable Infrastructure for Multidrug Resistant Organism Surveillance
Authors: Gazzarata Roberta , Monteverde Maria Eugenia , Ruggiero Carmelina , Maggi Norbert , Palmieri Dalia , Parruti Giustino , Giacomini Mauro
Published: January 2020

Prevention and surveillance of healthcare associated infections caused by multidrug resistant organisms (MDROs) has been given increasing attention in recent years and is nowadays a major priority for health care systems. The creation of automated regional, national and international surveillance networks plays a key role in this respect. A surveillance system has been designed for the Abruzzo region in Italy, focusing on the monitoring of the MDROs prevalence in patients, on the appropriateness of antibiotic prescription in hospitalized patients and on foreseeable interactions with other networks at national and international level. The system has been designed according to the Service Oriented Architecture (SOA) principles, and Healthcare Service Specification (HSSP) standards and Clinical Document Architecture Release 2 (CDAR2) have been adopted. A description is given with special reference to implementation state, specific design and implementation choices and next foreseeable steps. The first release will be delivered at the Complex Operating Unit of Infectious Diseases of the Local Health Authority of Pescara (Italy).
The Effect of Upgrades to Childcare Outdoor Spaces on Preschoolers’ Physical Activity: Findings from a Natural Experiment
Authors: Ng Michelle , Rosenberg Michael , Thornton Ashleigh , Lester Leanne , Trost Stewart G. , Bai Pulan , Christian Hayley
Published: January 2020

Physical inactivity is a significant risk factor for childhood obesity. Preventing obesity in the early years reduces the risk of developing chronic health conditions later. Early childhood education and care (ECEC) services are important settings to establish good preschooler physical activity behaviors. This natural experiment investigated the influence of ECEC outdoor physical environment upgrade on preschoolers’ physical activity (aged 2–5 years). Centers implemented upgrades without researcher input. Physical activity was measured by 7-day accelerometry for intervention (n = 159; 6 centers) and control (n = 138; 5 centers) groups. ECEC outdoor space was assessed using a modified Environment and Policy Assessment and Observation (EPAO) Instrument. Key outcomes were measured at baseline and 6–12 months follow-up. Fixed sandboxes, balls, portable slides, portable floor play equipment (e.g., tumbling mats), and natural grassed areas were positively associated with activity levels; fixed tunnels and twirling equipment were negatively associated with activity levels (all p < 0.05). Post-upgrade portable play equipment (balls, twirling equipment, slides, floor play equipment) increased intervention preschoolers’ moderate-vigorous physical activity (MVPA) levels compared to control (p < 0.05). Intervention preschoolers were more active than control at follow-up (58.09 vs. 42.13 min/day increase in total physical activity; 30.46 vs. 19.16 min/day increase in MVPA (all p < 0.001)). Since few preschoolers meet daily activity recommendations while at ECEC, the findings may help ECEC providers to optimize outdoor physical environments and encourage more active play among preschoolers.
Functional Expression and One-Step Protein Purification of Manganese Peroxidase 1 (rMnP1) from Phanerochaete chrysosporium Using the E. coli-Expression System
Authors: Pech-Canul Angel De La Cruz , Carrillo-Campos Javier , Ballinas-Casarrubias María de Lourdes , Solis-Oviedo Rosa Lidia , Hernández-Rascón Selena Karina , Hernández-Ochoa León Raúl , Gutiérrez-Méndez Néstor , García-Triana Antonio
Published: January 2020

Manganese peroxidases (MnP) from the white-rot fungi Phanerochaete chrysosporium catalyse the oxidation of Mn2+ to Mn3+, a strong oxidizer able to oxidize a wide variety of organic compounds. Different approaches have been used to unravel the enzymatic properties and potential applications of MnP. However, these efforts have been hampered by the limited production of native MnP by fungi. Heterologous expression of MnP has been achieved in both eukaryotic and prokaryotic expression systems, although with limited production and many disadvantages in the process. Here we described a novel molecular approach for the expression and purification of manganese peroxidase isoform 1 (MnP1) from P. chrysosporium using an E. coli-expression system. The proposed strategy involved the codon optimization and chemical synthesis of the MnP1 gene for optimised expression in the E. coli T7 shuffle host. Recombinant MnP1 (rMnP1) was expressed as a fusion protein, which was recovered from solubilised inclusion bodies. rMnP1 was purified from the fusion protein using intein-based protein purification techniques and a one-step affinity chromatography. The designated strategy allowed production of an active enzyme able to oxidize guaiacol or Mn2+.
Mucopolysaccharidosis-Plus Syndrome
Authors: Vasilev Filipp , Sukhomyasova Aitalina , Otomo Takanobu
Published: January 2020

Previously, we reported a novel disease of impaired glycosaminoglycans (GAGs) metabolism without deficiency of known lysosomal enzymes—mucopolysaccharidosis-plus syndrome (MPSPS). MPSPS, whose pathophysiology is not elucidated, is an autosomal recessive multisystem disorder caused by a specific mutation p.R498W in the VPS33A gene. VPS33A functions in endocytic and autophagic pathways, but p.R498W mutation did not affect both of these pathways in the patient’s skin fibroblast. Nineteen patients with MPSPS have been identified: seventeen patients were found among the Yakut population (Russia) and two patients from Turkey. Clinical features of MPSPS patients are similar to conventional mucopolysaccharidoses (MPS). In addition to typical symptoms for conventional MPS, MPSPS patients developed other features such as congenital heart defects, renal and hematopoietic disorders. Diagnosis generally requires evidence of clinical picture similar to MPS and molecular genetic testing. Disease is very severe, prognosis is unfavorable and most of patients died at age of 10–20 months. Currently there is no specific therapy for this disease and clinical management is limited to supportive and symptomatic treatment.
New Insights into the Structure-Function Relationship of the Endosomal-Type Na+, K+/H+ Antiporter NHX6 from Mulberry (Morus notabilis)
Authors: Cao Boning , Xia Zhongqiang , Liu Changying , Fan Wei , Zhang Shuai , Liu Qiao , Xiang Zhonghuai , Zhao Aichun
Published: January 2020

The endosomal-type Na+, K+/H+ antiporters (NHXs) play important roles in K+, vesicle pH homeostasis, and protein trafficking in plant. However, the structure governing ion transport mechanism and the key residues related to the structure–function of the endosomal-type NHXs remain unclear. Here, the structure-function relationship of the only endosomal-type NHX from mulberry, MnNHX6, was investigated by homology modeling, mutagenesis, and localization analyses in yeast. The ectopic expression of MnNHX6 in arabidopsis and Nhx1 mutant yeast can enhance their salt tolerance. MnNHX6’s three-dimensional structure, established by homology modeling, was supported by empirical, phylogenetic, and experimental data. Structure analysis showed that MnNHX6 contains unusual 13 transmembrane helices, but the structural core formed by TM5-TM12 assembly is conserved. Localization analysis showed that MnNHX6 has the same endosomal localization as yeast Nhx1/VPS44, and Arg402 is important for protein stability of MnNHX6. Mutagenesis analysis demonstrated MnNHX6 contains a conserved cation binding mechanism and a similar charge-compensated pattern as NHE1, but shares a different role in ion selectivity than the vacuolar-type NHXs. These results improve our understanding of the role played by the structure–function related key residues of the plant endosomal-type NHXs, and provide a basis for the ion transport mechanism study of endosomal-type NHXs.
The EGFR-ERK/JNK-CCL20 Pathway in Scratched Keratinocytes May Underpin Koebnerization in Psoriasis Patients
Authors: Furue Kazuhisa , Ito Takamichi , Tanaka Yuka , Hashimoto-Hachiya Akiko , Takemura Masaki , Murata Maho , Kido-Nakahara Makiko , Tsuji Gaku , Nakahara Takeshi , Furue Masutaka
Published: January 2020

Epidermal keratinocytes represent a rich source of C-C motif chemokine 20 (CCL20) and recruit CCR6+ interleukin (IL)-17A–producing T cells that are known to be pathogenic for psoriasis. A previous study revealed that scratch injury on keratinocytes upregulates CCL20 production, which is implicated in the Koebner phenomenon characteristically seen in psoriasis patients. However, the molecular mechanisms leading to scratch-induced CCL20 production remain elusive. In this study, we demonstrate that scratch injury upregulates the phosphorylation of epidermal growth factor receptor (EGFR) and that the specific EGFR inhibitor PD153035 attenuates scratch-induced CCL20 upregulation in an extracellular signal-related kinase (ERK)-dependent, and to a lesser extent, a c-Jun N-terminal kinase (JNK)-dependent but p38 mitogen-activated protein kinase (MAPK)–independent manner. Immunoreactive CCL20 was visualized in the keratinocytes that lined the scratched wound. IL-17A also induced the phosphorylation of EGFR and further augmented scratch-induced CCL20 upregulation. The EGFR-ERK/JNK-CCL20 pathway in scratched keratinocytes may explain why Koebnerization is frequently seen in psoriasis patients.
3D Structure and Function of Glycosyltransferases Involved in N-glycan Maturation
Authors: Nagae Masamichi , Yamaguchi Yoshiki , Taniguchi Naoyuki , Kizuka Yasuhiko
Published: January 2020

Glycosylation is the most ubiquitous post-translational modification in eukaryotes. N-glycan is attached to nascent glycoproteins and is processed and matured by various glycosidases and glycosyltransferases during protein transport. Genetic and biochemical studies have demonstrated that alternations of the N-glycan structure play crucial roles in various physiological and pathological events including progression of cancer, diabetes, and Alzheimer’s disease. In particular, the formation of N-glycan branches regulates the functions of target glycoprotein, which are catalyzed by specific N-acetylglucosaminyltransferases (GnTs) such as GnT-III, GnT-IVs, GnT-V, and GnT-IX, and a fucosyltransferase, FUT8s. Although the 3D structures of all enzymes have not been solved to date, recent progress in structural analysis of these glycosyltransferases has provided insights into substrate recognition and catalytic reaction mechanisms. In this review, we discuss the biological significance and structure-function relationships of these enzymes.
Epigallocatechin-3-Gallate Suppresses Vasculogenic Mimicry through Inhibiting the Twist/VE-Cadherin/AKT Pathway in Human Prostate Cancer PC-3 Cells
Authors: Yeo Changhwan , Han Deok-Soo , Lee Hyo-Jeong , Lee Eun-Ok
Published: January 2020

Vasculogenic mimicry (VM) is the alternative process of forming vessel-like networks by aggressive tumor cells, and it has an important role in tumor survival, growth, and metastasis. Epigallocatechin-3-gallate (EGCG) is well known to have diverse bioactivities including anti-cancer effects. However, the efficacy of EGCG on VM is elusive. In this study, we explored whether and how EGCG affects VM in human prostate cancer (PCa) PC-3 cells. Cell viability was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Invasive and VM formation abilities were assessed by an invasion assay and a three-dimensional (3D) culture VM tube formation assay, respectively. Western blots were carried out. An immunofluorescence assay was performed to detect nuclear twist expression. EGCG effectively inhibited the invasive ability, as well as tubular channel formation, without affecting cell viability. EGCG significantly downregulated the expression of vascular endothelial cadherin (VE-cadherin) and its transcription factor, twist, N-cadherin, vimentin, phosphor-AKT, and AKT, but not phospho-erythropoietin-producing hepatocellular receptor A2 (EphA2) and EphA2. In addition, EGCG diminished the nuclear localization of twist. Treatment with SC79, an AKT activator, effectively rescued EGCG-inhibited VM formation. These results demonstrated for the first time that EGCG causes marked suppression of VM through inhibiting the twist/VE-cadherin/AKT pathway in human PCa PC-3 cells.
Molecular Mechanism of Vegetative Growth Advantage in Allotriploid Populus
Authors: Du Kang , Liao Ting , Ren Yongyu , Geng Xining , Kang Xiangyang
Published: January 2020

Allotriploid poplar has a prominent vegetative growth advantage that impacts dramatically on lumber yield. The growth regulation is complex which involves abundant genes, metabolic and signaling pathways, while the information about the functional control process is very little. We used high-throughput sequencing and physiological index measurement to obtain a global overview of differences between allotriploid and diploid Populus. The genes related to plant growth advantage show a higher expression compared to diploid, and most of them are revolved around hormones, photosynthesis and product accumulation. Thus, allotriploid Populus showed more efficient photosynthesis, carbon fixation, sucrose and starch synthesis, and metabolism as well as augmented biosynthesis of auxin, cytokinin, and gibberellin. These data enable the connection of metabolic processes, signaling pathways, and specific gene activity, which will underpin the development of network models to elucidate the process of triploid Populus advantage growth.
Cell Cycle and DNA Repair Regulation in the Damage Response: Protein Phosphatases Take Over the Reins
Authors: Campos Adrián , Clemente-Blanco Andrés
Published: January 2020

Cells are constantly suffering genotoxic stresses that affect the integrity of our genetic material. Genotoxic insults must be repaired to avoid the loss or inappropriate transmission of the genetic information, a situation that could lead to the appearance of developmental abnormalities and tumorigenesis. To combat this threat, eukaryotic cells have evolved a set of sophisticated molecular mechanisms that are collectively known as the DNA damage response (DDR). This surveillance system controls several aspects of the cellular response, including the detection of lesions, a temporary cell cycle arrest, and the repair of the broken DNA. While the regulation of the DDR by numerous kinases has been well documented over the last decade, the complex roles of protein dephosphorylation have only recently begun to be investigated. Here, we review recent progress in the characterization of DDR-related protein phosphatases during the response to a DNA lesion, focusing mainly on their ability to modulate the DNA damage checkpoint and the repair of the damaged DNA. We also discuss their protein composition and structure, target specificity, and biochemical regulation along the different stages encompassed in the DDR. The compilation of this information will allow us to better comprehend the physiological significance of protein dephosphorylation in the maintenance of genome integrity and cell viability in response to genotoxic stress.
Comparison of The Sorption Kinetics of Lead(II) and Zinc(II) on Titanium Phosphate Ion-Exchanger
Authors: Maslova Marina V. , Ivanenko Vladimir I. , Yanicheva Nataliya Yu. , Mudruk Natalia V.
Published: January 2020

The treatment of heavy metal-contaminated wastewater is an important action to reduce The negative impacts of industrial wastes on water bodies. This work focuses on The application of a low-cost titanium (IV) phosphate sorbent of TiO(OH)H2PO42H2O chemical composition toward lead and zinc ions depending on their concentration and The temperature of The solution. The kinetic studies showed that The values of The rate of intraparticle diffusion and The effective diffusion coefficients for Zn2+ were considerably higher than those for Pb2+. To explain The difference between The sorption kinetics rates for Pb2+ and Zn2+, The effective radius and dehydration degree of The adsorbed ions were calculated. The sorbent capability of The lead and zinc ion removal and its excellent efficiency in The presence of a high concentration of calcium ions were demonstrated using simulated mine water. Due to The fast kinetics and The high exchange capacity of titanium phosphate toward divalent ions, this sorbent can be considered as a promising material for The concentration and immobilization of heavy metals into The phosphate matrix.
Upgrading Physical Layer of Multi-Carrier OGFDM Waveform for Improving Wireless Channel Capacity of 5G Mobile Networks and Beyond
Authors: Kadhum Mohammad R.
Published: January 2020

On the brink of sophisticated generations of mobile starting with the fifth-generation (5G) and moving on to the future mobile technologies, the necessity for developing the wireless telecommunications waveform is extremely required. The main reason beyond this is to support the future digital lifestyle that tends principally to maximize wireless channel capacity and number of connected users. In this paper, the upgraded design of the multi-carrier orthogonal generalized frequency division multiplexing (OGFDM) that aims to enlarge the number of mobile subscribers yet sustaining each one with a high transmission capacity is presented, explored, and evaluated. The expanded multi-carrier OGFDM can improve the performance of the future wireless network that targets equally the broad sharing operation (scalability) and elevated transmission rate. From a spectrum perspective, the upgraded OGFDM can manipulate the side effect of the increased number of network subscribers on the transmission bit-rate for each frequency subcarrier. This primarily can be achieved by utilizing the developed OGFDM features, like acceleration ability, filter orthogonality, interference avoidance, subcarrier scalability, and flexible bit loading. Consequently, the introduced OGFDM can supply lower latency, better BW efficiency, higher robustness, wider sharing, and more resilient bit loading than the current waveform. To highlight the main advantages of the proposed OGFDM, the system performance is compared with the initial design of the multicarrier OGFDM side by side with the 5G waveform generalized frequency division multiplexing (GFDM). The experimented results show that by moving from both the conventional OGFDM and GFDM with 4 GHz to the advanced OGFDM with 6 GHz, the gained channel capacity is improved. Because of the efficient use of Hilbert filters and improved rate of sampling acceleration, the upgraded system can gain about 3 dB and 1.5 dB increments in relative to the OGFDM and GFDM respectively. This, as a result, can maximize mainly the overall channel capacity of the enhanced OGFDM, which in turn can raise the bit-rate of each user in the mobile network. In addition, by employing the OGFDM with the dual oversampling, the achieved channel capacity in worst transmission condition is increased to around six and twelve times relative to the OGFDM and GFDM with the normal oversampling. Furthermore, applying the promoted OGFDM with the adaptive modulation comes up with maximizing the overall channel capacity up to around 1.66 dB and 3.32 dB compared to the initial OGFDM and GFDM respectively. A MATLAB simulation is applied to evaluate the transmission performance in terms of the channel capacity and the bit error rate (BER) in an electrical back-to-back wireless transmission system.
Optimal Feature Aggregation and Combination for Two-Dimensional Ensemble Feature Selection
Authors: Alhamidi Machmud Roby , Jatmiko Wisnu
Published: January 2020

Feature selection is a way of reducing the features of data such that, when the classification algorithm runs, it produces better accuracy. In general, conventional feature selection is quite unstable when faced with changing data characteristics. It would be inefficient to implement individual feature selection in some cases. Ensemble feature selection exists to overcome this problem. However, with the advantages of ensemble feature selection, some issues like stability, threshold, and feature aggregation still need to be overcome. We propose a new framework to deal with stability and feature aggregation. We also used an automatic threshold to see whether it was efficient or not; the results showed that the proposed method always produces the best performance in both accuracy and feature reduction. The accuracy comparison between the proposed method and other methods was 0.5–14% and reduced more features than other methods by 50%. The stability of the proposed method was also excellent, with an average of 0.9. However, when we applied the automatic threshold, there was no beneficial improvement compared to without an automatic threshold. Overall, the proposed method presented excellent performance compared to previous work and standard ReliefF.
A Rolling Electrical Generator Design and Model for Ocean Wave Energy Conversion
Authors: Damacharla Praveen , Fard Ali Jamali
Published: January 2020

Wave and tidal energies are some of the most prominent potential sources of renewable energy. Presently, these energy sources are not being utilized to their maximum extent. In this paper, we present a new conversion mechanism with an innovative electrical energy converter design that enables the use of wave energy to its maximum potential. The conventional wave energy converter comprises two stages of conversion (kinetic to mechanical and mechanical to electrical), imposing transformation loss that reduces the overall system efficiency. Additionally, the architecture and operational norms are dependent on the availability of shoreline areas, and the convertor is not suitable for all ocean weather conditions. To solve these problems, we have developed a wave energy conversion system that integrates the two stages of power with the minimum number of moving parts. This results in significant reduction of transformation losses that otherwise occur in the process. This paper presents an innovative idea of designing a DC generator that reduces the hierarchy of power conversion levels involved to improve the efficiency. The back and forth motion of the machine means it operates in a two-quadrant generation mode. The machine was constructed as a square box model with windings placed on both the top and bottom stator plates, and the rotor consisted of a field winding placed between these plates with two axes of operation. The electromagnetic field (EMF) induced in the stator plates is due to the resulting flux cutting, which is generated by a rolling object (rotor) in between them. A finite element analysis (FEA) of the machine is also listed to validate the flux linkage and operational efficiency. Additionally, a generator is fabricated to the predetermined design criteria as a proof of concept and the corresponding results are posted in the paper. Additionally, we present the material and cost limitations of this invention and outline some possible future directions.
Continuous Positive Airway Pressure Treatment in Patients with Alzheimer’s Disease: A Systematic Review
Authors: Perez-Cabezas Veronica , Ruiz-Molinero Carmen , Jimenez-Rejano Jose Jesus , Gonzalez-Medina Gloria , Galan-Mercant Alejandro , Martin-Valero Rocio
Published: January 2020

Background: Epidemiological studies have suggested a pathophysiological relationship between obstructive sleep apnea syndrome (OSAS) and Alzheimer’s disease (AD). The aim of this study is to evaluate the treatment of obstructive sleep apnea with continuous positive airway pressure (CPAP) in AD and its relationship with neurocognitive function improvement. Methods: Systematic review conducted following PRISMA’s statements. Relevant studies were searched in MEDLINE, PEDro, SCOPUS, PsycINFO, Web of Science, CINAHL and SportDicus. Original studies in which CPAP treatment was developel in AD patients have been included. Results: 5 studies, 3 RCTs (Randomized controlled trials) and 2 pilot studies. In all RCTs the CPAP intervention was six weeks; 3 weeks of therapeutic CPAP vs. 3 weeks placebo CPAP (pCPAP) followed by 3 weeks tCPAP in patients with AD and OSA. The two pilot studies conducted a follow-up in which the impact on cognitive impairment was measured. Conclusions: CPAP treatment in AD patients decreases excessive daytime sleepiness and improves sleep quality. There are indications that cognitive deterioration function measured with the Mini Mental Scale decreases or evolves to a lesser extent in Alzheimer’s patients treated with CPAP. Caregivers observe stabilization in disease progression with integration of CPAP. More research is needed on the topic presented.
Maternal Serum Placental Growth Factor, Soluble Fms-Like Tyrosine Kinase-1, and Soluble Endoglin in Twin Gestations and the Risk of Preeclampsia—A Systematic Review
Authors: Kosinska-Kaczynska Katarzyna , Zgliczynska Magdalena , Kozlowski Szymon , Wicherek Lukasz
Published: January 2020

Multiple gestation is one of the key risk factors for the occurrence of preeclampsia (PE). Soluble fms-like tyrosine kinase-1, placental growth factor, and soluble endoglin are molecules involved in the process of angiogenesis with a proven role in the pathogenesis of PE. The aim of the review was to summarize available data on maternal serum levels of the above-mentioned factors and their usefulness in predicting PE in twin pregnancies. Only original research articles written in English were considered eligible. Reviews, chapters, case studies, conference papers, experts’ opinions, editorials, and letters were excluded from the analysis. No publication date limitations were imposed. The systematic literature search using PubMed/MEDLINE, Scopus, Embase, and Cochrane Library databases identified 338 articles, 10 of which were included in the final qualitative analyses. The included studies showed significant differences in maternal serum levels of the discussed factors between women with twin pregnancies with PE and those who did not develop PE, and their promising performance in predicting PE, alone or in combination with other factors. The identification of the most effective algorithms, their prompt introduction to the clinical practice, and further assessment of the real-life performance should become a priority.
The Immunomodulary Effects of Systematic Exercise in Older Adults and People with Parkinson’s Disease
Authors: Szymura Jadwiga , Kubica Jadwiga , Wiecek Magdalena , Pera Joanna
Published: January 2020

We sought to investigate whether regular balance training of moderate intensity (BT) has an effect on changes in selected cytokines, neurotrophic factors, CD200 and fractalkine in healthy older adults and participants with Parkinson’s disease (PD). Sixty-two subjects were divided into groups depending on experimental intervention: (1) group of people with PD participating in BT (PDBT), (2) group of healthy older people participating in BT (HBT), (3,4) control groups including healthy individuals (HNT) and people with PD (PDNT). Blood samples were collected twice: before and after 12 weeks of balance exercise (PDBT, HBT), or 12 weeks apart (PDNT, HNT). The study revealed significant increase of interleukin10 (PDBT, p = 0.026; HBT, p = 0.011), β-nerve growth factor (HBT, p = 0.002; PDBT, p = 0.016), transforming growth factor-β1 (PDBT, p = 0.018; HBT, p < 0.004), brain-derived neurotrophic factor (PDBT, p = 0.011; HBT, p < 0.001) and fractalkine (PDBT, p = 0.045; HBT, p < 0.003) concentration only in training groups. In PDBT, we have found a significant decrease of tumor necrosis factor alpha. No training effect on concentration of interleukin6, insulin-like growth factor 1 and CD200 was observed in both training and control groups. Regular training can modulate level of inflammatory markers and induce neuroprotective mechanism to reduce the inflammatory response.
Characterization and Analysis of the Skin Microbiota in Rosacea: Impact of Systemic Antibiotics
Authors: Woo Yu Ri , Lee Se Hoon , Cho Sang Hyun , Lee Jeong Deuk , Kim Hei Sung
Published: January 2020

Systemic antibiotics are extensively used to control the papules and pustules of rosacea. Hence, it is crucial to understand their impact on the rosacea skin microbiota which is thought to be perturbed. The purpose of this study was to compare the makeup and diversity of the skin microbiota in rosacea before and after taking oral antibiotics. We also compared the skin microbiota at baseline according to age and rosacea severity. A longitudinal cohort study was performed on 12 rosacea patients with papules/pustules and no recent use of oral and topical antimicrobials/retinoids. Patients were prescribed oral doxycycline, 100 mg, twice daily for six weeks. Skin areas on the cheek and nose were sampled for 16S ribosomal RNA gene sequencing at baseline, and after six weeks of doxycycline treatment. Eleven females and one male aged 20–79 (median 51) with a median Investigator’s Global Assessment score of 3 (moderate) were enrolled. At baseline, Staphylococcus epidermidis was the most dominant species followed by Cutibacterium acnes (formerly Propionibacterium acnes). In the 60 Over-age group, the prevalence of Cutibacterium acnes was lower than that of the 60 & Under-age group. Rosacea severity increased with age and was associated with a decrease in the relative abundance of Cutibacterium acnes and an increase of Snodgrassella alvi. Across all subjects, antibiotic treatment reduced clinical rosacea grades and was associated with an increase in the relative abundance of Weissella confusa (P = 0.008, 95% CI 0.13% to 0.61%). Bacterial diversity (alpha diversity) was not significantly altered by antibiotics treatment. Principal coordinates analysis showed mild clustering of samples by patient (ANOSIM, Analysis of Similarity, R = 0.119, P = 0.16) and scant clustering with treatment (ANOSIM, R = 0.002; P = 0.5). In conclusion, we believe that rosacea has a unique age-dependent characteristic (i.e., severity). Although we were not able to pinpoint a causative microbiota, our study provides a glimpse into the skin microbiota in rosacea and its modulation by systemic antibiotics.
Sequential Circulating Tumor Cell Counts in Patients with Locally Advanced or Metastatic Hepatocellular Carcinoma: Monitoring the Treatment Response
Authors: Rau Kun-Ming , Liu Chien-Ting , Hsiao Yu-Chiao , Hsiao Kai-Yin , Wang Tzu-Min , Hung Wei-Shan , Su Yu-Li , Liu Wei-Ching , Wang Cheng-Hsu , Hsu Hsueh-Ling , Chuang Po-Heng , Cheng Ju-Chien , Tseng Ching-Ping
Published: January 2020

Hepatocellular carcinoma (HCC) is among the most common causes of cancer death in men. Whether or not a longitudinal follow-up of circulating tumor cells (CTCs) before and at different time points during systemic/targeted therapy is useful for monitoring the treatment response of patients with locally advanced or metastatic HCC has been evaluated in this study. Blood samples (n = 104) were obtained from patients with locally advanced or metastatic HCC (n = 30) for the enrichment of CTCs by a negative selection method. Analysis of the blood samples from patients with defined disease status (n = 81) revealed that those with progressive disease (PD, n = 37) had significantly higher CTC counts compared to those with a partial response (PR) or stable disease (SD; n = 44 for PR + SD, p = 0.0002). The median CTC count for patients with PD and for patients with PR and SD was 50 (interquartile range 21–139) and 15 (interquartile range 4–41) cells/mL of blood, respectively. A longitudinal analysis of patients (n = 17) after a series of blood collections demonstrated that a change in the CTC count correlated with the patient treatment response in most of the cases and was particularly useful for monitoring patients without elevated serum alpha-fetoprotein (AFP) levels. Sequential CTC enumeration during treatment can supplement standard medical tests and benefit the management of patients with locally advanced or metastatic HCC, in particular for the AFP-low cases.
An Efficient Photodynamic Therapy Treatment for Human Pancreatic Adenocarcinoma
Authors: Quilbe Alexandre , Moralès Olivier , Baydoun Martha , Kumar Abhishek , Mustapha Rami , Murakami Takashi , Leroux Bertrand , de Schutter Clémentine , Thecua Elise , Ziane Laurine , Colombeau Ludovic , Frochot Céline , Mordon Serge , Delhem Nadira
Published: January 2020

To date, pancreatic adenocarcinoma (ADKP) is a devastating disease for which the incidence rate is close to the mortality rate. The survival rate has evolved only 2–5% in 45 years, highlighting the failure of current therapies. Otherwise, the use of photodynamic therapy (PDT), based on the use of an adapted photosensitizer (PS) has already proved its worth and has prompted a growing interest in the field of oncology. We have developed a new photosensitizer (PS-FOL/PS2), protected by a recently published patent (WO2019 016397-A1, 24 January 2019). This photosensitizer is associated with an addressing molecule (folic acid) targeting the folate receptor 1 (FOLR1) with a high affinity. Folate binds to FOLR1, in a specific way, expressed in 100% of ADKP or over-expressed in 30% of cases. The first objective of this study is to evaluate the effectiveness of this PS2-PDT in four ADKP cell lines: Capan-1, Capan-2, MiapaCa-2, and Panc-1. For this purpose, we first evaluated the gene and protein expression of FOLR1 on four ADKP cell lines. Subsequently, we evaluated PS2’s efficacy in our cell lines and we assessed the impact of PDT on the secretome of cancer cells and its impact on the immune system. Finally, we evaluate the PDT efficacy on a humanized SCID mouse model of pancreatic cancer. In a very interesting way, we observed a significant increase in the proliferation of activated-human PBMC when cultured with conditioned media of ADKP cancer cells subjected to PDT. Furthermore, to evaluate in vivo the impact of this new PS, we analyzed the tumor growth in a humanized SCID mice model of pancreatic cancer. Four conditions were tested: Untreated, mice (nontreated), mice with PS (PS2), mice subjected to illumination (Light only), and mice subjected to illumination in the presence of PS (PDT). We noticed that the mice subjected to PDT presented a strong decrease in the growth of the tumor over time after illumination. Our investigations have not only suggested that PS2-PDT is an effective therapy in the treatment of PDAC but also that it activates the immune system and could be considered as a real adjuvant for anti-cancer vaccination. Thus, this new study provides new treatment options for patients in a therapeutic impasse and will provide a new arsenal in the fight against PDAC.
A Computational Approach to the Prediction of the Floating Condition of ROPAX Vessel after Firewater Accumulation in Firefighting Operation
Authors: Wang Honggui , Wu Zhaolin
Published: January 2020

A reliable estimation of the floating condition of a roll on/roll off cargo (ROPAX) vessel after the accumulation of firewater on the vehicle deck is extremely important for making correct decisions on evacuation and abandonment. Thus, for the seafarer working on a ROPAX vessel, there is a demand for a time-dependent prediction of the floating condition of the vessel after the firewater accumulation. For this purpose, a new iterative computational approach, based on quasi-static theory, is presented. The approach is examined through the records observed in an accident of the M/V Dashun. The results show that the approach has good accuracy and feasibility provided that the actual heeling angle and cargo shift during the accident are carefully monitored.
Does off-Farm Migration of Female Laborers Inhibit Land Transfer? Evidence from Sichuan Province, China
Authors: Huang Kai , Deng Xin , Liu Yi , Yong Zhuolin , Xu Dingde
Published: January 2020

With the feminization of agriculture, the role of women in the rural land transfer market is becoming increasingly important. However, at present, there is little research focusing on the relationship between the off-farm migration of female laborers and land transfer rates. Using data on 1652 agricultural land plots owned by 232 rural households in Sichuan Province in 2014, IV-Probit (The Probit model of tool variable method is added) and IV-Tobit (The Tobit model of tool variable method is added) models were constructed to explore the relationships between off-farm migration and rural household land transfer (whether the rural households have land transfer-in and the area of land transfer-in by rural households) with consideration of gender. The results show that: (1) Off-farm labor migration has a negative and significant impact on rural households’ land transfer-in rates. Under the same conditions, the off-farm migration rate increased by 10%, the probability of transfer-in land decreased by 2.6% on average, and the transfer-in area decreased by 2.7 mu on average. (2) The off-farm migration of female laborers inhibited land transfer-in rates. For every 10% increase in female labor migration, the probability of land transfer-in decreased by an average of 2.1%, and the land transfer-in area was reduced by an average of 3 mu (1 mu = 667 m2 or 0.067 ha). However, the impact of male labor migration on farmers’ land transfer-in is negative and not significant. This study provides a novel, gendered perspective to understand land transfer-in behaviors in hilly areas, which can provide further information on off-farm labor migration and the rational allocation of land resources.
Hydrological Control of Vegetation Greenness Dynamics in Africa: A Multivariate Analysis Using Satellite Observed Soil Moisture, Terrestrial Water Storage and Precipitation
Authors: Ugbaje Sabastine , Bishop Thomas
Published: January 2020

Vegetation activity in many parts of Africa is constrained by dynamics in the hydrologic cycle. Using satellite products, the relative importance of soil moisture, rainfall, and terrestrial water storage (TWS) on vegetation greenness seasonality and anomaly over Africa were assessed for the period between 2003 and 2015. The possible delayed response of vegetation to water availability was considered by including 0–6 and 12 months of the hydrological variables lagged in time prior to the vegetation greenness observations. Except in the drylands, the relationship between vegetation greenness seasonality and the hydrological measures was generally strong across Africa. Contrarily, anomalies in vegetation greenness were generally less coupled to anomalies in water availability, except in some parts of eastern and southern Africa where a moderate relationship was evident. Soil moisture was the most important variable driving vegetation greenness in more than 50% of the areas studied, followed by rainfall when seasonality was considered, and by TWS when the monthly anomalies were used. Soil moisture and TWS were generally concurrent or lagged vegetation by 1 month, whereas precipitation lagged vegetation by 1–2 months. Overall, the results underscore the pre-eminence of soil moisture as an indicator of vegetation greenness among satellite measured hydrological variables.