bluuebunny/crossref_metadata_2025 huggingface dataset:
Multiracial Britishness
Authors: Kong Vivian
Published: October 2023

What does it mean to be British? To answer this, Multiracial Britishness takes us to an underexplored site of Britishness – the former British colony of Hong Kong. Vivian Kong asks how colonial hierarchies, the racial and cultural diversity of the British Empire, and global ideologies complicate the meaning of being British. Using multi-lingual sources and oral history, Kong traces the experiences of multiracial residents in 1910-45 Hong Kong. Guiding us through Hong Kong's global networks, and the colony's co-existing exclusive and cosmopolitan social spaces, this book uncovers the long history of multiracial Britishness. Kong argues that Britishness existed in the colony in multiple, hyphenated forms – as a racial category, but also as privileges, a means of survival, and a form of cultural and national belonging. This book offers us an important reminder that multiracial inhabitants of the British Empire were just as active in the making of Britishness as the British state and white Britons.
Caricature and Realism in the Romantic Novel
Authors: Ferguson Olivia
Published: October 2023

What was caricature to novelists in the Romantic period? Why does Jane Austen call Mr Dashwood's wife 'a strong caricature of himself'? Why does Mary Shelley describe the body of Frankenstein's creature as 'in proportion', but then 'distorted in its proportions' – and does caricature have anything to do with it? This book answers those questions, shifting our understanding of 'caricature' as a literary-critical term in the decades when 'the English novel' was first defined and canonised as a distinct literary entity. Novels incorporated caricature talk and anti-caricature rhetoric to tell readers what different realisms purported to show them. Recovering the period's concept of caricature, Caricature and Realism in the Romantic Novel sheds light on formal realism's self-reflexivity about the 'caricature' of artifice, exaggeration and imagination. This title is part of the Flip it Open Programme and may also be available Open Access. Check our website Cambridge Core for details.
International Law
Authors: Klabbers Jan
Published: October 2023

Written by one of the world's leading international lawyers, this is a landmark publication in the teaching of international law. International law can be defined as 'the rules governing the legal relationship between nations and states'. However, with political, diplomatic and socio-economic factors shaping the law and its application, international law is much more complex. This refreshingly clear, concise textbook encourages students to view international law as a dynamic system of organising the world. Bringing international law back to its first principles and breathing new life and energy into the subject, the book is organised around four questions: Where does it come from? To whom does it apply? How does it resolve conflict? What does it say? This fourth edition includes references to new case-law and literature, and features (brief) discussions on recent topics of general interest, including the Ukraine invasion, global health law and energy law.
Systems, Relations, and the Structures of International Societies
Authors: Donnelly Jack
Published: October 2023

Inspired by recent work in evolutionary, developmental, and systems biology, Systems, Relations, and the Structures of International Societies sketches a robust conception of systems that grounds a new conception of levels (of organization, not merely analysis). Understanding international systems as multi-level multi-actor complex adaptive systems allows explanations of important features of the world that are inaccessible to dominant causal and rationalist explanatory strategies. It also develops a comprehensive critique of IR's dominant conception of systems and structures (narrow, rigid, and unfruitful); presents a novel conception of the interrelationship of the social production of continuities and the social production of change; and sketches models of spatio-political structure that cast new light on the development of international systems, including a distinctive account of the nature of globalization.
Legacy
Authors: Helm Dieter
Published: October 2023

What would a sustainable economy look like? What would it take to live within our environmental means? Legacy answers these and other questions, setting out the key features of the sustainable economy. It explains what it would take to properly maintain different types of capital, why polluters would have to pay, why the current generation would have to fund the necessary maintenance of our natural assets, and why we would have to save to invest. The message is a tough one: we are way off course in terms of meeting these conditions and we cannot escape the consequences. This book explains what we would have to do to mend our ways. In doing so, it highlights the feebleness of current approaches to net zero and biodiversity loss as well as our great neglect of the core infrastructures, and why we are not meeting our duties to the next generation. This title is Open Access.
Remarks on the collection of Byzantine lead seals of the Barber Institute of Fine Arts (University of Birmingham): mobility, networks, and identity in eastern Pontos
Authors: Malatras Christos
Published: October 2023

The Byzantine lead seals of the Barber Institute of Fine Arts number in total 146 pieces, mostly collected in the region of Trebizond. They offer valuable insights into the middle Byzantine society of the Pontos region, which despite its location on the easternmost borders was connected with other, even more remote, regions of the empire. The majority of these seals come from local officials and reflect their local preoccupations, perhaps as a backlash to the dominant culture of the capital. Fifteen selected pieces from the collection are published here and provided with commentary.
Flavour, emulsifiers and colour are the most frequent markers to detect food ultra-processing in a UK food market analysis
Authors: Neumann Nathalie Judith , Eichner Gerrit , Fasshauer Mathias
Published: October 2023

Objective:

To elucidate which markers of ultra-processing (MUP) and their combinations are best suited to detect ultra-processed food (UPF).

Design:

The study was based on the 206 food and 32 beverage items of the Oxford WebQ which encompass all major foods consumed in the UK. For each Oxford WebQ question, ingredient lists of up to ten matching different commercial products (n 2146) were researched online using data from the two market leaders of groceries in the UK sorted by relevance (Tesco) and by top sellers (Sainsbury’s), respectively. According to the NOVA classification, sixty-five MUP were defined, and if the ingredient list of a food product was positive for at least one MUP, it was regarded as UPF. The percentage of UPF items containing specific MUP was calculated. In addition, all combinations of two to six different MUP were assessed concerning the percentage of identified UPF items.

Setting:

Cross-sectional analysis.

Participants:

None.

Results:

A total of 990 products contained at least one MUP and were, therefore, regarded as UPF. The most frequent MUP were flavour (578 items, 58·4 % of all UPF), emulsifiers (353 items, 35·7 % of all UPF) and colour (262 items, 26·5 % of all UPF). Combined, these three MUP detected 79·2 % of all UPF products. Detection rate increased to 88·4 % of all UPF if ingredient lists were analysed concerning three additional MUP, that is, fibre, dextrose and firming agent.

Conclusions:

Almost 90 % of all UPF items can be detected by six MUP.
Tree Growth Enhancement Drives a Persistent Biomass Gain in Unmanaged Temperate Forests
Authors: Marqués Laura , Weng Ensheng , Bugmann Harald , Forrester David I. , Rohner Brigitte , Hobi Martina L. , Trotsiuk Volodymyr , Stocker Benjamin D.
Published: October 2023

While enhanced tree growth over the last decades has been reported in forests across the globe, it remains unclear whether it drives persistent biomass increases of forest stands, particularly in mature forests. Enhanced tree growth and stand‐level biomass are often linked with a simultaneous increase in density‐driven mortality and a reduction in tree longevity. Identifying empirical evidence regarding the balance between these processes is challenging due to the confounding effects of stand history, management, and environmental changes. Here, we investigate the link between growth and biomass via the negative relationship between average tree size and stand density (tree number per area). We find increasing stand density for a given mean tree size in unmanaged closed‐canopy forests in Switzerland over the past six decades and a positive relationship between tree growth and stand density across forest plots—qualitatively consistent with our simulations using a mechanistic, cohort‐resolving ecosystem model (BiomeE). Model simulations show that, in the absence of other disturbances, enhanced tree growth persistently increases biomass stocks despite simultaneous decreases in carbon residence time and tree longevity. However, the magnitude of simulated biomass changes for a given growth enhancement critically depends on the shape of the mortality functions. Our analyses reconcile reports of growth‐induced reductions of tree longevity with model predictions of persistent biomass increases, and with our finding of trends toward denser forests in response to growth—also in mature stands.
Shallow Tectonic Stress Magnitudes at the Hikurangi Subduction Margin, New Zealand
Authors: Behboudi E. , McNamara D. D. , Lokmer I.
Published: October 2023

Quantifying tectonic stress magnitudes is crucial in understanding crustal deformation processes, fault geomechanics, and variable plate interface slip behaviors in subduction zones. The Hikurangi Subduction Margin (HSM), New Zealand, is characterized by along‐strike variation in interface slip behavior, which may be linked to tectonic stress variations within the overriding plate. This study constrains in situ stress magnitudes of the shallow (<3 km) overriding plate of the HSM to better understand its tectonics and how they relate to larger scale subduction dynamics. Results reveal σ3: Sv ratios of 0.6–1 at depths above 650–700 m TVD and 0.92–1 below this depth interval along the entire HSM. Additionally, for depths below 650–700 m TVD, SHmax: Sv ratios of 0.95–1.81 in the central HSM and 0.95–2.3 in the southern HSM are estimated. These stress ratios suggest a prevalent thrust to strike‐slip (σ1 = SHmax) faulting regime across the central and southern HSM. In the central HSM, the presence of NNE‐NE striking reverse faults co‐existing with a modern σ1 (SHmax) aligned ENE‐WSW suggests that overtime the stress state here evolved from a contractional to a strike‐slip state, where the compressional direction changes from perpendicular (NW‐SE) to oblique (ENE‐WSW) to the Hikurangi margin. This temporal change in stress state may be explained by forearc rotation, likely combined with the development of upper plate overpressures. In the southern HSM, the modern WNW‐ESE/NW‐SE σ1 (SHmax) and pre‐existing NNE‐NE striking reverse faults indicate that stress state remains contractional and perpendicular (NW‐SE) to the Hikurangi margin overtime.
Global Hydrogen Production During High‐Pressure Serpentinization of Subducting Slabs
Authors: Merdith A. S. , Daniel I. , Sverjensky D. , Andreani M. , Mather B. , Williams S. , Vitale Brovarone A.
Published: October 2023

Serpentinization is among the most important, and ubiquitous, geological processes in crustal–upper mantle conditions (<6 GPa, <600°C), altering the rheology of rocks and producing H2 that can sustain life. While observations are available to quantify serpentinization in terrestrial and mid‐ocean ridge environments, measurements within subduction zone environments are far more sparse. To overcome this difficulty, we design a methodology to quantify and offer a first‐order estimate of the magnitude of “slab‐serpentinization” that has occurred over the last 5 Ma within the world's subduction zones by coupling four discrete tectonic and geophysical datasets—(a) raster grids of relic abyssal peridotite (peridotite exhumed from slow spreading mid‐ocean ridges but unaffected by pre‐subduction serpentinization) within ocean basins, (b) slab geometry, (c) thermal profiles and a (d) plate‐tectonic model. Averaged per year, our results suggest that 4.2–24 • 107 kg of H2 per annum could be generated from “slab‐serpentinization” within a subduction zone. Our estimate is 1–2 orders of magnitude lower than what is thought to be produced at mid‐ocean ridges, and of a similar magnitude to what could occur when including serpentinization at trench flexure and possible mantle wedge serpentinization. Higher hydrogen production is correlated most strongly with the spreading history of ocean basins, underlaying the importance of the tectonic history of a slab prior to subduction.
The Influence of Solar Irradiation and Solar Wind Conditions on Heavy Ion Escape from Mars
Authors: Zhang Qi , Holmström Mats , Wang Xiao‐dong , Nilsson Hans , Barabash Stas
Published: October 2023

We apply a recently proposed method to estimate heavy ion escape from Mars. The method combines in situ observations with a hybrid plasma model, which treats ions as particles and electrons as a fluid. With this method, we investigate how solar upstream conditions, including solar extreme ultraviolet (EUV) radiation, solar wind dynamic pressure, and interplanetary magnetic field (IMF) strength and cone angle, affect the heavy ion loss. The results indicate that the heavy ion escape rate is greater in high EUV conditions. The escape rate increases with increasing solar wind dynamic pressure, and decreases as the IMF strength increases. The ion escape rate is highest when the solar wind is parallel to the IMF and lowest when they are perpendicular. The plume escape rate decreases when the solar wind convective electric field increases.
Magnetic Longitudinal and Local Time Variations of Polar Electrojet and Field‐Aligned Currents
Authors: Wang Hui , Lühr Hermann
Published: October 2023

In this work, prominent longitudinal variation of polar electrojet (PEJ) and field‐aligned currents (FACs) are reported for the first time in different local times and hemispheres. Larger longitudinal differences exist mostly in the south than in the north with nearly opposite phase except for the nighttime. The most prominent longitudinal variation occurs around noontime. In the nighttime, the longitudinal changes of PEJ and upward FACs in both hemispheres are similar. The larger northern summer FACs and PEJ at noon occur more frequently around 0°–60° and 300°–360° magnetic longitude (MLon), while the larger southern summer currents occur around 120°–300° MLon. The summer‐winter hemispheric differences in PEJ and upward FACs disappear in the midnight sector. The northern FACs dominance exists around 0°–120° and 270°–360° MLon, except for winter when the northern dominance of upward FACs at night exist in nearly all longitudes. In local summer, PEJ shows northern dominance around 0°–60° and 270°–360° MLon, and southern dominance around 120°–240° MLon. In local winter, PEJ shows northern dominance around 180° MLon near midnight, while in most other local times, PEJ shows northern dominance around 270° MLon. These longitudinal, seasonal and hemispheric differences are discussed in terms of mixing effects of ionospheric conductance and merging electric field in different local time sectors.
A single session of aerobic exercise reduces systolic blood pressure at rest and in response to stress in women with rheumatoid arthritis and hypertension
Authors: de Luna Tatiane Almeida , Rezende Diego Augusto Nunes , de Brito Leandro Campos , Fecchio Rafael Yokoyama , Lima Fernanda Rodrigues , de Sá Pinto Ana Lúcia , de Medeiros Ribeiro Ana Cristina , Bonfiglioli Karina Rossi , Gualano Bruno , Roschel Hamilton , Peçanha Tiago
Published: October 2023

Rheumatoid arthritis (RA) is an autoimmune inflammatory disease characterized by increased risk of cardiovascular disease and hypertension (HT). A single session of aerobic exercise may reduce blood pressure (BP) in different clinical groups; however, little is known about the acute effects of exercise on BP in RA patients. This is a randomized controlled crossover study that assessed the effects of a single session of aerobic exercise on resting BP, on BP responses to stressful stimuli, and on 24-h BP in women with RA and HT. Twenty women with RA and HT (53 ± 10 years) undertook sessions of 30-min treadmill exercise (50% VO2max) or control (no exercise) in a crossover fashion. Before and after the sessions, BP was measured at rest, and in response to the Stroop-Color Word Test (SCWT), the Cold Pressor Test (CPT), and an isometric handgrip test. After the sessions, participants were also fitted with an ambulatory BP monitor for the assessment of 24-h BP. A single session of exercise reduced resting systolic BP (SBP) (−5 ± 9 mmHg; p < 0.05), and reduced SBP response to the SCWT (−7 ± 14 mmHg; p < 0.05), and to the CPT (−5 ± 11 mmHg; p < 0.05). Exercise did not reduce resting diastolic BP (DBP), BP responses to the isometric handgrip test or 24-h BP. In conclusion, a single session of aerobic exercise reduced SBP at rest and in response to stressful stimuli in hypertensive women with RA. These results support the use of exercise as a strategy for controlling HT and, hence, reducing cardiovascular risk in women with RA.

Clinical Trial Registration: This study registered at the Brazilian Clinical Trials (https://ensaiosclinicos.gov.br/rg/RBR-867k9g) at 12/13/2019.
Plasma endocannabinoids in cocaine dependence and their relation to cerebral metabotropic glutamate receptor 5 density
Authors: Kroll Sara L. , Hulka Lea M. , Kexel Ann-Kathrin , Vonmoos Matthias , Preller Katrin H. , Treyer Valerie , Ametamey Simon M. , Baumgartner Markus R. , Boost Carola , Pahlisch Franziska , Rohleder Cathrin , Leweke F. Markus , Quednow Boris B.
Published: October 2023

Animal models indicate that the endocannabinoid system (ECS) plays a modulatory role in stress and reward processing, both crucially impaired in addictive disorders. Preclinical findings showed endocannabinoid-modulated synaptic plasticity in reward brain networks linked to the metabotropic-glutamate-5 receptor (mGluR5), contributing to drug-reinforcing effects and drug-seeking behavior. Although animal models postulate a link between ECS and cocaine addiction, human translational studies are lacking. Here, we tested previous preclinical findings by investigating plasma endocannabinoids (eCBs) anandamide (AEA), 2-arachidonoylglycerol (2-AG), and the related N-acylethanolamines (NAEs) palmitoylethanolamide (PEA) and oleoylethanolamide (OEA), including their interaction with cerebral mGluR5, in chronic cocaine users (CU). We compared basal plasma concentrations between chronic CU (N = 103; 69 recreational CU and 34 dependent CU) and stimulant-naïve healthy controls (N = 92). Follow-up basal eCB/NAE plasma levels after 12 months were used for reliability and stability check (CU:N = 33; controls:N = 43). In an additional analysis using11C-ABP688 positron emission tomography (PET) in a male subsample (CU:N = 18; controls:N = 16), we investigated the relationships between eCBs/NAEs and mGluR5 density in the brain. We found higher 2-AG plasma levels in dependent CU compared to controls and recreational CU. 2-AG levels were stable over time across all groups. In the PET-subsample, a positive association between 2-AG and mGluR5 brain density only in CU was found. Our results corroborate animal findings suggesting an alteration of the ECS in cocaine dependence and an association between peripheral 2-AG levels and cerebral mGluR5 in humans. Therefore, the ECS might be a promising pharmaco-therapeutic target for novel treatments of cocaine dependence.
Dopaminergic denervation and associated MRI microstructural changes in the nigrostriatal projection in early Parkinson’s disease patients
Authors: López-Aguirre M. , Matarazzo M. , Blesa J. , Monje M. H. G. , Rodríguez-Rojas R. , Sánchez-Ferro A. , Obeso J. A. , Pineda-Pardo J. A.
Published: October 2023

Loss of dopaminergic neurons in the substantia nigra pars compacta (SNc) and a profound reduction of striatal dopamine are two hallmarks of Parkinson’s disease (PD). However, it’s unclear whether degeneration starts at the neuronal soma or the striatal presynaptic terminals, and how microstructural degeneration is linked to dopaminergic loss is also uncertain. In this study, thirty de novo PD patients and twenty healthy subjects (HS) underwent 6-[18F]-fluoro-L-dopa (FDOPA) PET and MRI studies no later than 12 months from clinical diagnosis. FDOPA uptake rate (Ki), fractional volume of free-water (FW), and iron-sensitive R2* relaxometry were quantified within nigrostriatal regions. Inter-group differences (PD vs HS) were studied using non-parametric statistics and complemented with Cohen’s d effect sizes and Bayesian statistics. Correlation analyses were performed exploring biomarker dependencies and their association with bradykinesia scores. PD patients exhibited a significant decline in nigrostriatal dopaminergic activity, being post-commissural putamen (−67%) and posterolateral SNc (−11.7%) the most affected subregions within striatum and SNc respectively. Microstructural alterations (FW) were restricted to the hemisphere corresponding to the most affected side and followed similar spatial gradients as FDOPA Ki (+20% in posterior putamen and +11% in posterolateral SNc). R2* revealed no relevant significant changes. FDOPA and FW were correlated within the posterolateral SNc, and clinical severity was associated with FDOPA Ki loss. The asymmetry between striatal and SNc changes for both dopaminergic depletion and microstructural degeneration biomarkers is consistent with a neurodegenerative process that begins in the striatal terminals before progressing toward the cell bodies in the SNc.
Non-invasive temporal interference electrical stimulation of the human hippocampus
Authors: Violante Ines R. , Alania Ketevan , Cassarà Antonino M. , Neufeld Esra , Acerbo Emma , Carron Romain , Williamson Adam , Kurtin Danielle L. , Rhodes Edward , Hampshire Adam , Kuster Niels , Boyden Edward S. , Pascual-Leone Alvaro , Grossman Nir
Published: October 2023

Deep brain stimulation (DBS) via implanted electrodes is used worldwide to treat patients with severe neurological and psychiatric disorders. However, its invasiveness precludes widespread clinical use and deployment in research. Temporal interference (TI) is a strategy for non-invasive steerable DBS using multiple kHz-range electric fields with a difference frequency within the range of neural activity. Here we report the validation of the non-invasive DBS concept in humans. We used electric field modeling and measurements in a human cadaver to verify that the locus of the transcranial TI stimulation can be steerably focused in the hippocampus with minimal exposure to the overlying cortex. We then used functional magnetic resonance imaging and behavioral experiments to show that TI stimulation can focally modulate hippocampal activity and enhance the accuracy of episodic memories in healthy humans. Our results demonstrate targeted, non-invasive electrical stimulation of deep structures in the human brain.
A study of thermal performance enhancement of building envelopes by using corncob as construction material
Authors: Huang Yi , Xu Chong , Wang Liang
Published: October 2023

The energy-saving design of buildings effectively reduces heating energy consumption, improves indoor environmental problems in traditional houses, and plays an essential role in alleviating the increasingly severe environmental and energy problems. This study reviews the status of research on building energy-saving renovation and environmental improvement in various countries and summarizes their relevant research experiences. It described the theories and methods of survey, experiment, and numerical simulation to provide a theoretical basis for energy-saving renovation and environmental improvement of residential houses. Furthermore, it introduces corncob concrete material to provide new materials for the renovation of an old building. This research is based on the coastal Liyuan houses in Qingdao, and the problems of Liyuan houses are identified through in-field investigation. Then, through experimental simulations, the application and efficiency of corncob materials in the thermal performance improvement of the envelope are explored. The renovation of old residential houses in terms of energy-saving and environmental improvement is proposed from envelope renovation.
A novel WGF-LN based edge driven intelligence for wearable devices in human activity recognition
Authors: Menaka S. R. , Prakash M. , Neelakandan S. , Radhakrishnan Arun
Published: October 2023

Human activity recognition (HAR) is one of the key applications of health monitoring that requires continuous use of wearable devices to track daily activities. The most efficient supervised machine learning (ML)-based approaches for predicting human activity are based on a continuous stream of sensor data. Sensor data analysis for human activity recognition using conventional algorithms and deep learning (DL) models shows promising results, but evaluating their ambiguity in decision-making is still challenging. In order to solve these issues, the paper proposes a novel Wasserstein gradient flow legonet WGF-LN-based human activity recognition system. At first, the input data is pre-processed. From the pre-processed data, the features are extracted using Haar Wavelet mother- Symlet wavelet coefficient scattering feature extraction (HS-WSFE). After that, the interest features are selected from the extracted features using (Binomial Distribution integrated-Golden Eagle Optimization) BD-GEO. The important features are then post-processed using the scatter plot matrix method. Obtained post-processing features are finally given into the WGF-LN for classifying human activities. From these experiments, the results can be obtained and showed the efficacy of the proposed model.
Neuroprotective potential of intranasally delivered L-myc immortalized human neural stem cells in female rats after a controlled cortical impact injury
Authors: Amirbekyan Mari , Adhikarla Vikram , Cheng Jeffrey P. , Moschonas Eleni H. , Bondi Corina O. , Rockne Russell C. , Kline Anthony E. , Gutova Margarita
Published: October 2023

Efficacious stem cell-based therapies for traumatic brain injury (TBI) depend on successful delivery, migration, and engraftment of stem cells to induce neuroprotection. L-myc expressing human neural stem cells (LMNSC008) demonstrate an inherent tropism to injury sites after intranasal (IN) administration. We hypothesize that IN delivered LMNSC008 cells migrate to primary and secondary injury sites and modulate biomarkers associated with neuroprotection and tissue regeneration. To test this hypothesis, immunocompetent adult female rats received either controlled cortical impact injury or sham surgery. LMNSC008 cells or a vehicle were administered IN on postoperative days 7, 9, 11, 13, 15, and 17. The distribution and migration of eGFP-expressing LMNSC008 cells were quantified over 1 mm-thick optically cleared (CLARITY) coronal brain sections from TBI and SHAM controls. NSC migration was observed along white matter tracts projecting toward the hippocampus and regions of TBI. ELISA and Nanostring assays revealed a shift in tissue gene expression in LMNSC008 treated rats relative to controls. LMNSC008 treatment reduced expression of genes and pathways involved in inflammatory response, microglial function, and various cytokines and receptors. Our proof-of-concept studies, although preliminary, support the rationale of using intranasal delivery of LMNSC008 cells for functional studies in preclinical models of TBI and provide support for potential translatability in TBI patients.
Prevalence and control of hypertension in a high HIV-prevalence setting, insights from a population based study in Botswana
Authors: Mosepele Mosepele , Bennett Kara , Gaolathe Tendani , Makhema Joseph M. , Mmalane Mompati , Holme Molly Pretorius , Lebelonyane Refeletswe , Ometoruwa Omolola , Mills Lisa A. , Powis Kathleen M. , Leidner Jean , Jarvis Joseph N. , Tapela Neo M. , Masupe Tiny , Mokgatlhe Lucky , Triant Virginia A. , Wirth Kathleen E. , Moshomo Thato , Lockman Shahin
Published: October 2023

In a population-based representative sample of adults residing in 22 communities in Botswana, a southern African country with high HIV prevalence, 1 in 4 individuals had high blood pressure. High blood pressure was less prevalent in adults with HIV than without HIV. Sixty percent of persons with high blood pressure had not previously been diagnosed. Among individuals with a prior diagnosis of high blood pressure who reported being prescribed anti-hypertension medications, almost half had elevated blood pressure, irrespective of HIV-status. One-third of adults in this setting (mainly men) declined free non-invasive blood pressure assessments in their households. In conclusion, our study highlights alarmingly high hypertension rates in the community, with low levels of awareness and control, emphasizing the urgent need for community level BP screening and active management to reach recommended targets.
Body size and temperature affect metabolic and cardiac thermal tolerance in fish
Authors: Kraskura Krista , Hardison Emily A. , Eliason Erika J.
Published: October 2023

Environmental warming is associated with reductions in ectotherm body sizes, suggesting that larger individuals may be more vulnerable to climate change. The mechanisms driving size-specific vulnerability to temperature are unknown but are required to finetune predictions of fisheries productivity and size-structure community responses to climate change. We explored the potential metabolic and cardiac mechanisms underlying these body size vulnerability trends in a eurythermal fish, barred surfperch. We acutely exposed surfperch across a large size range (5–700 g) to four ecologically relevant temperatures (16 °C, 12 °C, 20 °C, and 22 °C) and subsequently, measured their metabolic capacity (absolute and factorial aerobic scopes, maximum and resting metabolic rates; AAS, FAS, MMR, RMR). Additionally, we estimated the fish’s cardiac thermal tolerance by measuring their maximum heart rates (fHmax) across acutely increasing temperatures. Barred surfperch had parallel hypoallometric scaling of MMR and RMR (exponent 0.81) and a weaker hypoallometric scaling of fHmax (exponent − 0.05) across all test temperatures. In contrast to our predictions, the fish’s aerobic capacity was maintained across sizes and acute temperatures, and larger fish had greater cardiac thermal tolerance than smaller fish. These results demonstrate that thermal performance may be limited by different physiological constraints depending on the size of the animal and species of interest.
Negative phototaxis in the photosymbiotic sea anemone Aiptasia as a potential strategy to protect symbionts from photodamage
Authors: Kishimoto Mariko , Gornik Sebastian G. , Foulkes Nicholas S. , Guse Annika
Published: October 2023

Photosymbiotic cnidarians generally seek bright environments so that their symbionts can be photosynthetically active. However, excess light may result in a breakdown of symbiosis due to the accumulation of photodamage in symbionts causing symbiont loss (bleaching). It is currently unknown if photosymbiotic cnidarians sense light only to regulate spawning time and to facilitate predation, or whether they also use their light-sensing capacities to protect their symbionts from photodamage. In this study, we examined how the sea anemone Aiptasia changes its behaviour when exposed to excess light. We reveal that Aiptasia polyps, when carrying symbionts, contract their bodies when exposed to high light intensities and subsequently migrate away in a direction perpendicular to the light source. Interestingly, this negative phototaxis was only evident under blue light and absent upon UV, green and red light exposure. Non-symbiotic Aiptasia did not exhibit this light response. Our study demonstrates that photosymbiotic Aiptasia polyps display negative phototactic behaviour in response to blue light, and that they also can perceive its direction, despite lacking specialized eye structures. We postulate that Aiptasia uses blue light, which penetrates seawater efficiently, as a general proxy for sunlight exposure to protect its symbionts from photodamage.
One-pot synthesis, computational chemical study, molecular docking, biological study, and in silico prediction ADME/pharmacokinetics properties of 5-substituted 1H-tetrazole derivatives
Authors: El-Sewedy Ahmed , El-Bordany Eman A. , Mahmoud Naglaa F. H. , Ali Kholoud A. , Ramadan Sayed K.
Published: October 2023

An efficient synthesis of 5-substituted 1H-tetrazoles was successfully achieved through one-pot multi-component condensation reactions of some aromatic aldehydes or indolin-2,3-dione with malononitrile and sodium azide using diverse reaction conditions to obtain considerable product yields. Furthermore, it has been achieved for the first time to construct desired products under neat condition. Molecular docking studies with CSNK2A1 receptor disclosed the lowest binding energy displayed by the dimethoxyphenyl derivative 4c with − 6.8687 kcal/mol. The synthesized tetrazoles were screened for their in-vitro cytotoxic activity against epidermoid cancer cell line (A431) and colon cancer line (HCT116) with respect to normal skin fibroblast cell line (BJ-1) using MTT assay, and antimicrobial activity against the bacteria: K. pneumonia, S. aureus, and the fungi: Candida albicans, as well as their antioxidant activity using 2,2-diphenyl-1-picrylhydrazyl assay. In addition, the toxicity of tetrazole derivative was assessed by determination of their approximate lethal dose fifty (LD50), calculated via an oral administration to rats, through measurement of ALT and bilirubin levels in serum. The antitumor results can suggest that the potent tetrazole derivative namely, 3-(3,4-dimethoxyphenyl)-2-(1H-tetrazol-5-yl)acrylonitrile (4c) could be a potential drug against epidermoid carcinoma. The antioxidant results indicated to tetrazoles exhibited great antioxidant properties even at very low doses. A molecular dynamics simulation was performed for the synthesized compounds (ligands) to investigate their tendency for binding with the active sites of protein.
Medication profiling in women with type 1 diabetes highlights the importance of adequate, guideline-based treatment in low-risk groups
Authors: Lithovius Raija , Mutter Stefan , Parente Erika B. , Mäkinen Ville-Petteri , Valo Erkka , Harjutsalo Valma , Groop Per-Henrik
Published: October 2023

Effective treatment may prevent kidney complications, but women might be underprescribed. Novel, data-driven insights into prescriptions and their relationship with kidney health in women with type 1 diabetes may help to optimize treatment. We identified six medication profiles in 1164 women from the FinnDiane Study with normal albumin excretion rate based on clusters of their baseline prescription data using a self-organizing map. Future rapid kidney function decline was defined as an annual estimated glomerular filtration rate (eGFR) loss > 3 ml/min/1.73 m2 after baseline. Two profiles were associated with future decline: Profile ARB with the highest proportion of angiotensin receptor blockers (odds ratio [OR] 2.75, P = 0.02) and highly medicated women in profile HighMed (OR 2.55, P = 0.03). Compared with profile LowMed (low purchases of all), profile HighMed had worse clinical characteristics, whereas in profile ARB only systolic blood pressure was elevated. Importantly, the younger women in profile ARB with fewer kidney protective treatments developed a rapid decline despite otherwise similar baseline characteristics to profile ACE & Lipids (the highest proportions of ACE inhibitors and lipid-modifying agents) without a future rapid decline. In conclusion, medication profiles identified different future eGFR trajectories in women with type 1 diabetes revealing potential treatment gaps for younger women.
Antimicrobial potential and rhodamine B dye degradation using graphitic carbon nitride and polyvinylpyrrolidone doped bismuth tungstate supported with in silico molecular docking studies
Authors: Ashfaq Muhammad Hasnain , Imran Muhammad , Haider Ali , Shahzadi Anum , Mustajab Muhammad , Ul-Hamid Anwar , Nabgan Walid , Medina Francisco , Ikram Muhammad
Published: October 2023

The environmental-friendly hydrothermal method has been carried out to synthesize Bi2WO6 and g-C3N4/PVP doped Bi2WO6 nanorods (NRs) by incorporating different concentrations of graphitic carbon nitride (g-C3N4) as well as a specified quantity of polyvinylpyrrolidone (PVP). Bi2WO6 doped with g-C3N4 provides structural and chemical stability, reduces charge carriers, degrades dyes, and, owing to lower bandgap energy, is effective for antibacterial, catalytic activity, and molecular docking analysis. The purpose of this research is the treatment of polluted water and to investigate the bactericidal behavior of a ternary system. The catalytic degradation was performed to remove the harmful rhodamine B (RhB) dye using NaBH4 in conjunction with prepared NRs. The specimen compound demonstrated antibacterial activity against Escherichia coli (E. coli) at both high and low concentrations. Higher doped specimens of g-C3N4/PVP-doped Bi2WO6 exhibited a significant improvement in efficient bactericidal potential against E. coli (4.55 mm inhibition zone). In silico experiments were carried out on enoyl-[acylcarrier-protein] reductase (FabI) and β-lactamase enzyme for E. coli to assess the potential of Bi2WO6, PVP doped Bi2WO6, and g-C3N4/PVP-doped Bi2WO6 NRs as their inhibitors and to justify their possible mechanism of action.
Genetic diversity and population structure of maize inbred lines using phenotypic traits and single nucleotide polymorphism (SNP) markers
Authors: Dube Sweetbird Phindile , Sibiya Julia , Kutu Funso
Published: October 2023

Understanding germplasm’s genetic diversity is essential for developing new and improved cultivars with stable yields under diverse environments. The objective of this study was to determine the genetic diversity and population structure of 128 maize inbred lines sourced from the International Institute of Tropical Agriculture (IITA), the International Maize and Wheat Improvement Centre (CIMMYT), and the University of KwaZulu-Natal (UKZN) using 11,450 informative single nucleotide polymorphism (SNP) markers. The inbred lines revealed highly significant (p < 0.001) levels of variability for the key phenotypic traits. The SNP markers had a mean gene diversity (GD) and polymorphic information content (PIC) of 0.40 and 0.31, respectively, indicating the existence of substantial genetic variation across the germplasm panel. The model-based population structure analysis identified three subpopulations (K = 3) among the inbred lines. This corroborated the phylogenetic analysis using phenotypic traits and molecular markers which classified the inbred lines into three groups. The findings of this study identified considerable genetic diversity for the selection of inbred lines with favourable alleles for multiple traits and could be useful to initiate marker-assisted selection (MAS) to identify significant loci associated with agronomic performance and multiple-stress tolerance.
Flow study of Dean’s instability in high aspect ratio microchannels
Authors: Wong Yu Ching , Dai Cheng , Xian Qingyue , Yan Zhaoxu , Zhang Ziyi , Wen Weijia
Published: October 2023

Dean’s flow and Dean’s instability have always been important concepts in the inertial microfluidics. Curved channels are widely used for applications like mixing and sorting but are limited to Dean’s flow only. This work first reports the Dean’s instability flow in high aspect ratio channels on the deka-microns level for $$De>162$$

D
e
>
162

. A new channel geometry (the tortuous channel), which creates a rolled-up velocity profile, is presented and studied experimentally and numerically along with other three typical channel geometries at Dean’s flow condition and Dean’s instability condition. The tortuous channel generates a higher De environment at the same Re compared to the other channels and allows easier Dean’s instability creation. We further demonstrate the use of multiple vortexes for mixing. The mixing efficiency is considered among different channel patterns and the tortuous channel outperforms the others. This work offers more understanding of the creation of Dean’s instability at high aspect ratio channels and the effect of channel geometry on it. Ultimately, it demonstrates the potential for applications like mixing and cell sorting.
Miniaturized microscope for non-invasive imaging of leukocyte-endothelial interaction in human microcirculation
Authors: Bagramyan Arutyun , Lin Charles P.
Published: October 2023

We present a miniature oblique back-illumination microscope (mOBM) for imaging the microcirculation of human oral mucosa, enabling real-time, label-free phase contrast imaging of individual leukocytes circulating in the bloodstream, as well as their rolling and adhesion on vascular walls—the initial steps in leukocyte recruitment that is a hallmark of inflammation. Using the mOBM system, we studied the leukocyte-endothelial interactions in healthy and locally inflamed tissue and observed drastic changes in leukocyte movement (velocity and displacement profile). Our findings suggest that real-time imaging of leukocyte dynamics can provide new diagnostic insights (assessment of inflammation, temporal progression of disease, evaluation of therapeutic response, etc.) that are not available using conventional static parameters such as cell number and morphology.
Investigating PM2.5 toxicity in highly polluted urban and industrial areas in the Middle East: human health risk assessment and spatial distribution
Authors: Goodarzi Babak , Azimi Mohammadabadi Maryam , Jafari Ahmad Jonidi , Gholami Mitra , Kermani Majid , Assarehzadegan Mohammad-Ali , Shahsavani Abbas
Published: October 2023

Exposure to particulate matter (PM) can be considered as a factor affecting human health. The aim of this study was to investigate the concentration of PM2.5 and heavy metals and their influence on survival of A549 human lung cells in exposure to PM2.5 breathing air of Ahvaz city. In order to assess the levels of PM2.5 and heavy metals, air samples were collected from 14 sampling stations positioned across Ahvaz city during both winter and summer seasons. The concentration of heavy metals was determined using ICP OES. Next, the MTT assay [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] was employed to ascertain the survival rate of A549 cells. The findings from this research demonstrated that average PM2.5 of the study period was (149.5 μg/m3). Also, the average concentration of PM2.5 in the urban area in winter and summer was (153.3- and 106.9 μg/m3) and in the industrial area this parameter was (191.6 and 158.3 μg/m3). The average concentration of metals (ng/m3) of urban areas against industrial, Al (493 vs. 485), Fe (536 vs. 612), Cu (198 vs. 212), Ni (128 vs. 129), Cr (48.5 vs. 54), Cd (118 vs. 124), Mn (120 vs. 119), As (51 vs. 67), Hg (37 vs. 50), Zn (302 vs. 332) and Pb (266 vs. 351) were obtained. The results of the MTT assay showed that the highest percentage of cell survival according to the exposure concentration was 25 > 50 > 100 > 200. Also, the lowest percentage of survival (58.8%) was observed in the winter season and in industrial areas with a concentration of 200 μg/ml. The carcinogenic risk assessment of heavy metals indicated that except for Cr, whose carcinogenicity was 1.32E−03, other metals were in the safe range (10–4–10–6) for human health. The high concentration of PM2.5 and heavy metals can increase respiratory and cardiovascular diseases and reduce the public health level of Ahvaz citizens.
Artificial intelligence and the analysis of cryo-EM data provide structural insight into the molecular mechanisms underlying LN-lamininopathies
Authors: Kulczyk Arkadiusz W.
Published: October 2023

Laminins (Lm) are major components of basement membranes (BM), which polymerize to form a planar lattice on cell surface. Genetic alternations of Lm affect their oligomerization patterns and lead to failures in BM assembly manifesting in a group of human disorders collectively defined as Lm N-terminal domain lamininopathies (LN-lamininopathies). We have employed a recently determined cryo-EM structure of the Lm polymer node, the basic repeating unit of the Lm lattice, along with structure prediction and modeling to systematically analyze structures of twenty-three pathogenic Lm polymer nodes implicated in human disease. Our analysis provides the detailed mechanistic explanation how Lm mutations lead to failures in Lm polymerization underlining LN-lamininopathies. We propose the new categorization scheme of LN-lamininopathies based on the insight gained from the structural analysis. Our results can help to facilitate rational drug design aiming in the treatment of Lm deficiencies.
Risk factors for bronchopulmonary dysplasia infants with respiratory score greater than four: a multi-center, prospective, longitudinal cohort study in China
Authors: Xu Yan-ping , Chen Zheng , Dorazio Robert M. , Bai Guan-nan , Du Li-zhong , Shi Li-ping
Published: October 2023

Bronchopulmonary dysplasia (BPD) is the most common complication of prematurity involving both pre- and post-natal factors. A large, prospective, longitudinal cohort study was conducted to determine whether inflammation-related factors are associated with an increased risk of BPD in preterm infants who were born at a gestational age < 32 weeks, < 72 h after birth and respiratory score > 4. The study included infants from 25 participating hospitals in China between March 1, 2020 and March 31, 2022. The primary outcomes were BPD and severity of BPD at 36 weeks post-menstrual age. A total of 1362 preterm infants were enrolled in the study. After exclusion criteria, the remaining 1088 infants were included in this analysis, of whom, 588 (54.0%) infants were in the BPD group and 500 (46.0%) were in the non-BPD group. In the BPD III model, the following six factors were identified: birth weight (OR 0.175, 95% CI 0.060–0.512; p = 0.001), surfactant treatment (OR 8.052, 95% CI 2.658–24.399; p < 0.001), mean airway pressure (MAP) ≥ 12 cm H2O (OR 3.338, 95% CI 1.656–6.728; p = 0.001), late-onset sepsis (LOS) (OR 2.911, 95% CI 1.514–5.599; p = 0.001), ventilator-associated pneumonia (VAP) (OR 18.236, 95% CI 4.700–70.756; p < 0.001) and necrotizing enterocolitis (NEC) (OR 2.725, 95% CI 1.182–6.281; p = 0.019). Premature infants remained at high risk of BPD and with regional variation. We found that post-natal inflammation-related risk factors were associated with an increased risk of severe BPD, including LOS, VAP, NEC, MAP ≥ 12 cm H2O and use of surfactant.
Novelty detection in an auditory oddball task on freely moving rats
Authors: Quintela-Vega Laura , Morado-Díaz Camilo J. , Terreros Gonzalo , Sánchez Jazmín S. , Pérez-González David , Malmierca Manuel S.
Published: October 2023

The relative importance or saliency of sensory inputs depend on the animal’s environmental context and the behavioural responses to these same inputs can vary over time. Here we show how freely moving rats, trained to discriminate between deviant tones embedded in a regular pattern of repeating stimuli and different variations of the classic oddball paradigm, can detect deviant tones, and this discriminability resembles the properties that are typical of neuronal adaptation described in previous studies. Moreover, the auditory brainstem response (ABR) latency decreases after training, a finding consistent with the notion that animals develop a type of plasticity to auditory stimuli. Our study suggests the existence of a form of long-term memory that may modulate the level of neuronal adaptation according to its behavioural relevance, and sets the ground for future experiments that will help to disentangle the functional mechanisms that govern behavioural habituation and its relation to neuronal adaptation.