bluuebunny/crossref_metadata_2025 huggingface dataset:
An Innovative Low-Cost Equipment for Electro-Concentration of Microalgal Biomass
Authors: Sanchez-Galvis Edwar M. , Cardenas-Gutierrez Ingri Y. , Contreras-Ropero Jefferson E. , García-Martínez Janet B. , Barajas-Solano Andrés F. , Zuorro Antonio
Published: July 2020

Microalgal harvesting is one of the most challenging processes in the development of algal research and development. Several methods, such as centrifugation, flocculation and filtration, are available at the laboratory scale. However, the requirement for expensive pieces of equipment and the possibility of biomass contamination are recurring gaps that hinder the development of microalgae R&D (research and development) in different parts of the world. Recently, electroflotation has been proved to be a suitable method for the harvesting of different species of microalgae and cyanobacteria. To this day, there are no companies that sell laboratory-scale electroflotation equipment; this is mainly due to the gap in the knowledge of which factors (time, mixing rate, number of electrodes and others) will affect the efficiency of concentration without reducing the biomass quality. This paper aims to build an innovative, low-cost electroflotation system for under 300 USD (United States dollar) with cheap and resistant materials. To achieve our goal, we tested the interaction of three variables (time, mixing rate and amount of electrodes). Results showed that an efficiency closer to 100% could be achieved in under 20 min using > 10 electrodes and 150 rpm (round per minute). We hope this innovative approach can be used by different researchers to improve our knowledge of the concentration and harvesting of algae and cyanobacteria.
From Horrors Past to Horrors Future: Pacifist War Art (1919–1939)
Authors: Jannette Lauren
Published: July 2020

In this paper, I argue that interwar pacifists working in France presented an evolving narrative of what the First World War represented in order to maintain support for their movement and a continued peace in Europe. Utilizing posters, photographs, pamphlets, and art instillations created by pacifist organizations, I interject in ongoing debates over the First World War as a moment of rupture in art and pacifism in France, arguing that the moment of rupture occurred a decade after the conflict had ended with the failure of the Conference for the Reduction and Limitation of Armaments of 1932–1934 and the election of Hitler as the leader of a remilitarized Germany. Pacifist art of the 1920s saw a return to traditional motifs and styles of art that remembered the horrors of the past war. This return to tradition aimed to inspire adherence to the new pacifist organizations in the hopes of creating a new peace-filled world. The era of optimism and tradition ended with the economic and political crisis of the early 1930s, forcing pacifists to reconceptualize the images and styles of art that they utilized. Instead of relying on depictions of the horrors of the past war, these images shifted the focus to the mass civilian casualties future wars would bring in a desperate struggle to prevent the outbreak of another world war.
Automated Detection of Multi-Rotor UAVs Using a Machine-Learning Approach
Authors: Grác Šimon , Beňo Peter , Duchoň František , Dekan Martin , Tölgyessy Michal
Published: July 2020

The objective of this article is to propose and verify a reliable detection mechanism of multi-rotor unmanned aerial vehicles (UAVs). Such a task needs to be solved in many areas such as in the protection of vulnerable buildings or in the protection of privacy. Our system was firstly realized by standard computer vision methods using the Oriented FAST and Rotated BRIEF (ORB) feature detector. Due to the low success rate achieved in real-world conditions, the machine-learning approach was used as an alternative detection mechanism. The “Common Objects in Context dataset” was used as a predefined dataset and it was extended by 1000 samples of UAVs from the SafeShore dataset. The effectiveness and the reliability of our system are proven by four basic experiments—drone in a static image and videos which are displaying a drone in the sky, multiple drones in one image, and a drone with another flying object in the sky. The successful detection rate achieved was 97.3% in optimal conditions.
Wind Speed Forecast Based on Post-Processing of Numerical Weather Predictions Using a Gradient Boosting Decision Tree Algorithm
Authors: Xu Wenqing , Ning Like , Luo Yong
Published: July 2020

With the large-scale development of wind energy, wind power forecasting plays a key role in power dispatching in the electric power grid, as well as in the operation and maintenance of wind farms. The most important technology for wind power forecasting is forecasting wind speed. The current mainstream methods for wind speed forecasting involve the combination of mesoscale numerical meteorological models with a post-processing system. Our work uses the WRF model to obtain the numerical weather forecast and the gradient boosting decision tree (GBDT) algorithm to improve the near-surface wind speed post-processing results of the numerical weather model. We calculate the feature importance of GBDT in order to find out which feature most affects the post-processing wind speed results. The results show that, after using about 300 features at different height and pressure layers, the GBDT algorithm can output more accurate wind speed forecasts than the original WRF results and other post-processing models like decision tree regression (DTR) and multi-layer perceptron regression (MLPR). Using GBDT, the root mean square error (RMSE) of wind speed can be reduced from 2.7–3.5 m/s in the original WRF result by 1–1.5 m/s, which is better than DTR and MLPR. While the index of agreement (IA) can be improved by 0.10–0.20, correlation coefficient be improved by 0.10–0.18, Nash–Sutcliffe efficiency coefficient (NSE) be improved by −0.06–0.6. It also can be found that the feature which most affects the GBDT results is the near-surface wind speed. Other variables, such as forecast month, forecast time, and temperature, also affect the GBDT results.
The Study of Emission Inventory on Anthropogenic Air Pollutants and Source Apportionment of PM2.5 in the Changzhutan Urban Agglomeration, China
Authors: Xu Bin , You Xiangyu , Zhou Yaoyu , Dai Chunhao , Liu Zhan , Huang Shaojian , Luo Datong , Peng Hui
Published: July 2020

As one of China’s emerging urban agglomerations, the Changzhutan urban area is suffering from regional composite air pollution. Previous studies mainly focus on single cities or world-class urban agglomerations, which cannot provide a scientific basis for air pollution in emerging urban agglomerations. This paper proposes the latest high-resolution emission inventory through the emission factor method and compares the results with the rest of the urban agglomeration. The emission inventory shows that the estimates for sulfur dioxide (SO2), nitrogen oxides (NOX), particulate matter 10 (PM10), particulate matter 2.5 (PM2.5), volatile organic compounds (VOCs), and ammonia (NH3) emission are 132.5, 148.9, 111.6, 56.5, 119.0, and 72.0 kt, respectively. From the 3 × 3 km emission grid, the spatial difference of air pollutant emissions in the Changzhutan urban agglomeration was more obvious, but the overall trend of monthly pollutant discharge was relatively stable. Depending on the source apportionment, SO42−, OC, and NO3− are the main chemical constituents of PM2.5, accounting for 13.06, 8.24, and 4.84 μg/m3, respectively. Simultaneously, industrial emissions, vehicle exhaust, and dust are still three main sources that cannot be ignored. With the support of these data, the results of this study may provide a reference for other emerging urban agglomerations in air quality.
Interaction of a Low-Pressure System, an Offshore Trough, and Mid-Tropospheric Dry Air Intrusion: The Kerala Flood of August 2018
Authors: Kumar Vinay , Pradhan Prabodha Kumar , Sinha Tushar , Rao S. Vijaya Bhaskara , Chang Hao-Po
Published: July 2020

The present study examines the Kerala Flood Event (KFE, 15–16 August 2018, in India) that occurred along the west coast of India and resulted in ~400 mm of rainfall in one day. The KFE was unique in comparison to previous floods in India, not only due to the rainfall duration and amount, but also due to the fact that the dams failed to mitigate the flood, which made it the worst in history. The main goal of this study is to analyze and elucidate the KFE based on meteorological and hydrological parameters. A propagating low-pressure system (LPS) from the Bay of Bengal (BoB) caused the streak of plenty of rainfall over Kerala, the west coast, central India, and the BoB. Additionally, the upper-tropospheric anti-cyclonic system over the Middle East region inhibited a northward advancement of LPS. On the western coast of India, a non-propagating (with diurnal fluctuations) offshore trough was observed over the west coast (from Kerala to Gujarat state). Therefore, a synergic interaction between LPS, an intrusion of dry air in the middle-troposphere, and the offshore trough was the main reason for KFE. However, after around ten days, rainfall saturated the dam capacities; thus, the released water, along with the amount of precipitation on the day of the event, was one of the other possible reasons which worsened the flood over Kerala.
The Influence of the Mineral–Microbial Deodorizing Preparation on Ammonia Emission and Growth Performance in Turkey Production
Authors: Gałęcki Remigiusz , Dąbrowski Michał , Bakuła Tadeusz , Obremski Kazimierz , Baranowski Mirosław , Nowak Adriana , Gutarowska Beata
Published: July 2020

In our previous in vitro research and also in laying hen production, attempts were made to minimise ammonia emissions in poultry houses with the use of Deodoric® biopreparation. The objective of the present research was to evaluate the influence of the Deodoric® on ammonia (NH3) emission and turkey growth performance in a semi-industrial production system. Significant differences in NH3 emission (p-value < 0.001), body weight (p-value < 0.001) and relative humidity (p-value < 0.001) were observed between the control group (C) and the experimental group (E) where Deodoric® was applied. In group C, an increase in ammonia concentration in air could have contributed to a decrease in the body weight of turkeys, but the above correlation was not observed in group E. In the control group, a relatively strong correlation between NH3 emission and temperature (p-value = 0.0009; r = 0.74) and moderate correlations between NH3 emission vs. relative humidity (p-value = 0.01; r = 0.59), air speed (p-value = 0.015; r = 0.60) and cooling (p-value = 0.005; r = 0.66) were noted. Studied correlations were not observed in group E. The preparation did not affect microbial levels in manure or body samples. Throughout the experiment, significant differences in the number of mesophilic bacteria (for the model: F = 46.14, p-value = 0.09; for mesophilic microorganisms: F = 3.29, p-value = 0.045) and Campylobacter spp. (for the model: F = 24.96, p-value = 0.008; for Campylobacter spp.: F = 0.25, p-value = 0.64) were not observed between group C and group E. The administration of Deodoric® to manure decreased NH3 concentration in the air and increased weight gains in the experimental group of turkeys relative to group C. Preparation may be applied in poultry farms to improve poultry farming conditions.
Climatological Large-Scale Circulation Patterns over The Middle Americas Region
Authors: Ochoa-Moya Carlos A. , Cala-Pérez Yoel A. , Díaz-Esteban Yanet , Castro Christopher L. , Ordoñez-Peréz Paulina , Quintanar Arturo I.
Published: July 2020

In this study, twenty large-scale circulation patterns are identified to generate a synoptic classification of Weather Types (WT) over a region that comprises Mexico, the Intra-Americas Seas, Central America, and northern South America. This classification is performed using Self-Organizing Maps (SOMs) with mean sea-level pressure standardized anomalies from reanalysis. The influence of quasi-permanent pressure centers over the region, such as North Atlantic Subtropical High (NASH) and North Pacific High (NPH) are well captured. Seasonal variability of high-pressure centers for dry (November–April) and wet (May–October) periods over the entire region are also well represented in amplitude and pattern among the WTs. The NASH influence and intensification of the Caribbean low-level jet and the North American monsoon system is well captured. During the dry period, a strong trough wind advects cold air masses from mid-latitudes to the subtropics over the western Atlantic Ocean. High-frequency transitions among WTs tend to cluster around the nearest neighbors in SOM space, while low-frequency transitions occur along columns instead of rows in the SOM matrix. Low-frequency transitions are related to intraseasonal and seasonal scales. The constructed catalog can identify near-surface atmospheric circulation patterns from a unified perspective of synoptic climate variability, and it is in high agreement with previous studies for the region.
Cytotoxic, Antioxidant, and Enzyme Inhibitory Properties of the Traditional Medicinal Plant Matthiola incana (L.) R. Br.
Authors: Taviano Maria Fernanda , Miceli Natalizia , Acquaviva Rosaria , Malfa Giuseppe Antonio , Ragusa Salvatore , Giordano Deborah , Cásedas Guillermo , Les Francisco , López Víctor
Published: July 2020

Matthiola incana (L.) R. Br. (Brassicaceae) is widely cultivated for ornamental purposes and utilized as a medicinal plant. In the present work, the hydroalcoholic extract from the aerial parts of this species has been evaluated in different bioassays in order to detect potential pharmacological applications. The cytotoxic capacity against the human colorectal adenocarcinoma (CaCo-2) and breast cancer (MCF-7) cell lines was tested using the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. The extract was investigated as a neuroprotective inhibitor of central nervous system (CNS) enzymes such as monoamine oxidase A, tyrosinase, acetylcholinesterase, and as a natural enzyme inhibitor of α-glucosidase and lipase involved in some metabolic disorders such as obesity or type 2 diabetes. The antioxidant ability was also evaluated in an enzymatic system (xanthine/xanthine oxidase assay). Results showed that the M. incana extract displayed moderate to low cytotoxicity vs. CaCo-2 cells. The extract acted as a superoxide radical scavenger and enzymatic inhibitor of monoamine oxidase A, tyrosinase, α-glucosidase, and lipase. The best results were found in the α-glucosidase assay, as M. incana hydroalcoholic extract was able to inhibit the enzyme α-glucosidase up to 100% without significant differences, compared to the antidiabetic drug acarbose. Matthiola incana has been demonstrated to exert different biological properties. These are important in order to consider this species as a source of bioactive compounds.
Defensins of Grasses: A Systematic Review
Authors: Odintsova Tatyana I. , Slezina Marina P. , Istomina Ekaterina A.
Published: July 2020

The grass family (Poaceae) is one of the largest families of flowering plants, growing in all climatic zones of all continents, which includes species of exceptional economic importance. The high adaptability of grasses to adverse environmental factors implies the existence of efficient resistance mechanisms that involve the production of antimicrobial peptides (AMPs). Of plant AMPs, defensins represent one of the largest and best-studied families. Although wheat and barley seed γ-thionins were the first defensins isolated from plants, the functional characterization of grass defensins is still in its infancy. In this review, we summarize the current knowledge of the characterized defensins from cultivated and selected wild-growing grasses. For each species, isolation of defensins or production by heterologous expression, peptide structure, biological activity, and structure–function relationship are described, along with the gene expression data. We also provide our results on in silico mining of defensin-like sequences in the genomes of all described grass species and discuss their potential functions. The data presented will form the basis for elucidation of the mode of action of grass defensins and high adaptability of grasses to environmental stress and will provide novel potent molecules for practical use in medicine and agriculture.
Low Vitamin D States Observed in U.S. Marines and Navy Sailors with Early Multi-Symptom Illness
Authors: Maloney Sean R. , Goolkasian Paula
Published: July 2020

Research has implicated immune system inflammation as an underlying etiology of multi-symptom illnesses, and vitamin D has been shown to have a significant role in immune system function. In this retrospective review performed on the medical charts of service members who presented with signs and symptoms of multi-symptom illnesses, we focused on serum 25(OH)D3 levels and looked for associations of vitamin D status (deficient, insufficient, and normal) with age (20–31 years versus 31–56 years) and deployment status (war zones versus other). Two groups (U.S. Marines and Navy Sailors) were sampled and both showed high incidences of below normal vitamin D levels. However, with the Marines, age-related differences in serum levels (p = 0.009) were found only among those who deployed to Iraq/Afghanistan in comparison to those in non-combat locations. The comparison within the Navy sample showed that mobilized sailors had lower 25(OH)D3 levels than the group that did not deploy (p = 0.04). In addition, 100% of the sailors who deployed had below normal levels versus only 33% in the cadre group. The data suggest that personnel returning from a war zone with signs of early multi-symptom illness should be checked for low vitamin D status.
Anti-Inflammatory and Barrier-Stabilising Effects of Myrrh, Coffee Charcoal and Chamomile Flower Extract in a Co-Culture Cell Model of the Intestinal Mucosa
Authors: Weber Laura , Kuck Katrin , Jürgenliemk Guido , Heilmann Jörg , Lipowicz Bartosz , Vissiennon Cica
Published: July 2020

Recent clinical evidence suggests the efficacy of a traditional herbal medicinal product containing myrrh (Commiphora molmol Engl.), coffee charcoal (Coffea arabica L.) and chamomile flower dry extract (Matricaria chamomilla L.) in the therapy of inflammatory bowel diseases (IBD). However, the mechanisms of action in this context have not been entirely elucidated. The present study aimed to evaluate the effects of myrrh, coffee charcoal and chamomile flower extract on the inflammatory cross talk between immune and intestinal epithelial cells together with the resulting intestinal barrier disorders. A complex co-culture cell model consisting of intestinal epithelial cell (IEC) monolayers (Caco-2, HT29-MTX-E12) and macrophages (THP-1) was established for the simultaneous investigation of these two IBD characteristics. The lipopolysaccharide (LPS) activation of the macrophages led to a pro-inflammatory mediator release and thereby an inflammatory stimulation of IECs with chemokine release and reduced barrier function. The effects of the individual plant extracts and a ternary combination on inflammatory mediator release (IL-6, TNF, IL-8, MCP-1, PGE2) was quantified by ELISA. The transepithelial electrical resistance (TEER) of IEC monolayers was measured to evaluate the effects on the barrier function. Budesonide served as a positive control. All three plant extracts exhibited anti-inflammatory properties via the inhibition of the inflammatory mediator release to a varying extent. An intestinal barrier stabilising effect was observed for myrrh and coffee charcoal. Myrrh exerted the most distinct pharmacological activity. Dose reducing and synergistic interactions emerged within the threefold combination. Thus, our results provide a mechanistic basis for the use of the herbal combination of myrrh, coffee charcoal and chamomile flower extract in IBD treatment and underline the potential benefits of the phytotherapeutic multi-component/multi-target approach in this complex pathogenesis.
Attenuation of Inflammatory Symptoms by Icariside B2 in Carrageenan and LPS-Induced Inflammation Models via Regulation of MAPK/NF-κB Signaling Cascades
Authors: Alam Md Badrul , Kwon Yoon-Gyung , Simu Shakina Yesmin , Abrar Shahriyar Sk , Lee Sang Han
Published: July 2020

Prolonged inflammatory responses can lead to the development of several chronic diseases, such as autoimmune disorders and the development of natural therapeutic agents is required. A murine model was used to assess the anti-inflammatory effects of the megastigmane glucoside, icariside B2 (ICSB), and the assessment was carried out in vitro, and in vivo. The in vitro anti-inflammatory effects of ICSB were tested using LPS-stimulated BV2 cells, and the protein expression levels of inflammatory genes and cytokines were assessed. Mice were subcutaneously injected with 1% carrageenan (CA) to induce acute phase inflammation in the paw. Inflammation was assessed by measuring paw volumes hourly; subsequently, the mice were euthanized and the right hind paw skin was expunged and processed for reverse transcription-polymerase chain reaction (RT-PCR) and Western blot analyses. ICSB inhibits LPS-stimulated nitric oxide (NO) and prostaglandin E2 (PGE2) generation by reducing the expression of inducible NO synthase (iNOS) and cyclooxygenase 2 (COX-2). ICSB also inhibits the COX-2 enzyme with an IC50 value of 7.80 ± 0.26 µM. Molecular docking analysis revealed that ICSB had a strong binding affinity with both murine and human COX-2 proteins with binding energies of −8 kcal/mol and −7.4 kcal/mol, respectively. ICSB also reduces the manifestation of pro-inflammatory cytokines, such as TNF-α, IL-6, and IL-1β, at their transcriptional and translational level. ICSB hinders inhibitory protein κBα (IκBα) phosphorylation, thereby terminating the nuclear factor kappa-light-chain-enhancer of activated B cell (NF-κB) nuclear translocation. ICSB also represses the mitogen-activated protein kinases (MAPKs) signaling pathways. ICSB (50 mg/kg) showed an anti-edema effect in CA-induced mice and suppressed the CA-induced increases in iNOS and COX-2 protein levels. ICSB attenuated inflammatory responses by downregulating NF-κB expression through interference with extracellular signal-regulated kinase (ERK) and p38 phosphorylation, and by modulating the expression levels of iNOS, COX-2, TNF-α, IL-1β, and IL-6.
Biodegradable Flame Retardants for Biodegradable Polymer
Authors: Maqsood Muhammad , Seide Gunnar
Published: July 2020

To improve sustainability of polymers and to reduce carbon footprint, polymers from renewable resources are given significant attention due to the developing concern over environmental protection. The renewable materials are progressively used in many technical applications instead of short-term-use products. However, among other applications, the flame retardancy of such polymers needs to be improved for technical applications due to potential fire risk and their involvement in our daily life. To overcome this potential risk, various flame retardants (FRs) compounds based on conventional and non-conventional approaches such as inorganic FRs, nitrogen-based FRs, halogenated FRs and nanofillers were synthesized. However, most of the conventional FRs are non-biodegradable and if disposed in the landfill, microorganisms in the soil or water cannot degrade them. Hence, they remain in the environment for long time and may find their way not only in the food chain but can also easily attach to any airborne particle and can travel distances and may end up in freshwater, food products, ecosystems, or even can be inhaled if they are present in the air. Furthermore, it is not a good choice to use non-biodegradable FRs in biodegradable polymers such as polylactic acid (PLA). Therefore, the goal of this review paper is to promote the use of biodegradable and bio-based compounds for flame retardants used in polymeric materials.
Untargeted LC-MS Metabolomics Differentiates Between Virulent and Avirulent Clinical Strains of Pseudomonas aeruginosa
Authors: Depke Tobias , Thöming Janne Gesine , Kordes Adrian , Häussler Susanne , Brönstrup Mark
Published: July 2020

Pseudomonas aeruginosa is a facultative pathogen that can cause, inter alia, acute or chronic pneumonia in predisposed individuals. The gram-negative bacterium displays considerable genomic and phenotypic diversity that is also shaped by small molecule secondary metabolites. The discrimination of virulence phenotypes is highly relevant to the diagnosis and prognosis of P. aeruginosa infections. In order to discover small molecule metabolites that distinguish different virulence phenotypes of P. aeruginosa, 35 clinical strains were cultivated under standard conditions, characterized in terms of virulence and biofilm phenotype, and their metabolomes were investigated by untargeted liquid chromatography—mass spectrometry. The data was both mined for individual candidate markers as well as used to construct statistical models to infer the virulence phenotype from metabolomics data. We found that clinical strains that differed in their virulence and biofilm phenotype also had pronounced divergence in their metabolomes, as underlined by 332 features that were significantly differentially abundant with fold changes greater than 1.5 in both directions. Important virulence-associated secondary metabolites like rhamnolipids, alkyl quinolones or phenazines were found to be strongly upregulated in virulent strains. In contrast, we observed little change in primary metabolism. A hitherto novel cationic metabolite with a sum formula of C12H15N2 could be identified as a candidate biomarker. A random forest model was able to classify strains according to their virulence and biofilm phenotype with an area under the Receiver Operation Characteristics curve of 0.84. These findings demonstrate that untargeted metabolomics is a valuable tool to characterize P. aeruginosa virulence, and to explore interrelations between clinically important phenotypic traits and the bacterial metabolome.
Long Noncoding RNAs and Circular RNAs in Autoimmune Diseases
Authors: Lodde Valeria , Murgia Giampaolo , Simula Elena Rita , Steri Maristella , Floris Matteo , Idda Maria Laura
Published: July 2020

Immune responses are essential for the clearance of pathogens and the repair of injured tissues; however, if these responses are not properly controlled, autoimmune diseases can occur. Autoimmune diseases (ADs) are a family of disorders characterized by the body’s immune response being directed against its own tissues, with consequent chronic inflammation and tissue damage. Despite enormous efforts to identify new drug targets and develop new therapies to prevent and ameliorate AD symptoms, no definitive solutions are available today. Additionally, while substantial progress has been made in drug development for some ADs, most treatments only ameliorate symptoms and, in general, ADs are still incurable. Hundreds of genetic loci have been identified and associated with ADs by genome-wide association studies. However, the whole list of molecular factors that contribute to AD pathogenesis is still unknown. Noncoding (nc)RNAs, such as microRNAs, circular (circ)RNAs, and long noncoding (lnc)RNAs, regulate gene expression at different levels in various diseases, including ADs, and serve as potential drug targets as well as biomarkers for disease progression and response to therapy. In this review, we will focus on the potential roles and genetic regulation of ncRNA in four autoimmune diseases—systemic lupus erythematosus, rheumatoid arthritis, multiple sclerosis, and type 1 diabetes mellitus.
Role of RadA and DNA Polymerases in Recombination-Associated DNA Synthesis in Hyperthermophilic Archaea
Authors: Hogrel Gaëlle , Lu Yang , Alexandre Nicolas , Bossé Audrey , Dulermo Rémi , Ishino Sonoko , Ishino Yoshizumi , Flament Didier
Published: July 2020

Among the three domains of life, the process of homologous recombination (HR) plays a central role in the repair of double-strand DNA breaks and the restart of stalled replication forks. Curiously, main protein actors involved in the HR process appear to be essential for hyperthermophilic Archaea raising interesting questions about the role of HR in replication and repair strategies of those Archaea living in extreme conditions. One key actor of this process is the recombinase RadA, which allows the homologous strand search and provides a DNA substrate required for following DNA synthesis and restoring genetic information. DNA polymerase operation after the strand exchange step is unclear in Archaea. Working with Pyrococcus abyssi proteins, here we show that both DNA polymerases, family-B polymerase (PolB) and family-D polymerase (PolD), can take charge of processing the RadA-mediated recombination intermediates. Our results also indicate that PolD is far less efficient, as compared with PolB, to extend the invaded DNA at the displacement-loop (D-loop) substrate. These observations coincide with previous genetic analyses obtained on Thermococcus species showing that PolB is mainly involved in DNA repair without being essential probably because PolD could take over combined with additional partners.
Potential Endogenous Cell Sources for Retinal Regeneration in Vertebrates and Humans: Progenitor Traits and Specialization
Authors: Grigoryan Eleonora N.
Published: July 2020

Retinal diseases often cause the loss of photoreceptor cells and, consequently, impairment of vision. To date, several cell populations are known as potential endogenous retinal regeneration cell sources (RRCSs): the eye ciliary zone, the retinal pigment epithelium, the iris, and Müller glia. Factors that can activate the regenerative responses of RRCSs are currently under investigation. The present review considers accumulated data on the relationship between the progenitor properties of RRCSs and the features determining their differentiation. Specialized RRCSs (all except the ciliary zone in low vertebrates), despite their differences, appear to be partially “prepared” to exhibit their plasticity and be reprogrammed into retinal neurons due to the specific gene expression and epigenetic landscape. The “developmental” characteristics of RRCS gene expression are predefined by the pathway by which these cell populations form during eye morphogenesis; the epigenetic features responsible for chromatin organization in RRCSs are under intracellular regulation. Such genetic and epigenetic readiness is manifested in vivo in lower vertebrates and in vitro in higher ones under conditions permissive for cell phenotype transformation. Current studies on gene expression in RRCSs and changes in their epigenetic landscape help find experimental approaches to replacing dead cells through recruiting cells from endogenous resources in vertebrates and humans.
Reactive Metamizole Metabolites Enhance the Toxicity of Hemin on the ATP Pool in HL60 Cells by Inhibition of Glycolysis
Authors: Rudin Deborah , Schmutz Maurice , Roos Noëmi Johanna , Bouitbir Jamal , Krähenbühl Stephan
Published: July 2020

Metamizole is an analgesic, whose pharmacological and toxicological properties are attributed to N-methyl-aminoantipyrine (MAA), its major metabolite. In the presence of heme iron, MAA forms reactive metabolites, which are toxic for granulocyte precursors. Since decreased cellular ATP is characteristic for MAA-associated toxicity, we studied the effect of MAA with and without hemin on energy metabolism of HL60 cells, a granulocyte precursor cell line. The combination MAA/hemin depleted the cellular ATP stronger than hemin alone, whereas MAA alone was not toxic. This decrease in cellular ATP was observed before plasma membrane integrity impairment. MAA/hemin and hemin did not affect the proton leak but increased the maximal oxygen consumption by HL60 cells. This effect was reversed by addition of the radical scavenger N-acetylcysteine. The mitochondrial copy number was not affected by MAA/hemin or hemin. Hemin increased mitochondrial superoxide generation, which was not accentuated by MAA. MAA decreased cellular ROS accumulation in the presence of hemin. In cells cultured in galactose (favoring mitochondrial ATP generation), MAA/hemin had less effect on the cellular ATP and plasma membrane integrity than in glucose. MAA/hemin impaired glycolysis more than hemin or MAA alone, and N-acetylcysteine blunted this effect of MAA/hemin. MAA/hemin decreased protein expression of pyruvate kinase more than hemin or MAA alone. In conclusion, cellular ATP depletion appears to be an important mechanism of MAA/hemin toxicity on HL60 cells. MAA itself is not toxic on HL60 cells up to 100 µM but boosts the inhibitory effect of hemin on glycolysis through the formation of reactive metabolites.
Fluorescent Labeling of Helminth Extracellular Vesicles Using an In Vivo Whole Organism Approach
Authors: Boysen Anders T. , Whitehead Bradley , Stensballe Allan , Carnerup Anna , Nylander Tommy , Nejsum Peter
Published: July 2020

In the last two decades, extracellular vesicles (EVs) from the three domains of life, Archaea, Bacteria and Eukaryotes, have gained increasing scientific attention. As such, the role of EVs in host-pathogen communication and immune modulation are being intensely investigated. Pivotal to EV research is the determination of how and where EVs are taken up by recipient cells and organs in vivo, which requires suitable tracking strategies including labelling. Labelling of EVs is often performed post-isolation which increases risks of non-specific labelling and the introduction of labelling artefacts. Here we exploited the inability of helminths to de novo synthesise fatty acids to enable labelling of EVs by whole organism uptake of fluorescent lipid analogues and the subsequent incorporation in EVs. We showed uptake of 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (DOPE-Rho) in Anisakis spp. and Trichuris suis larvae. EVs isolated from the supernatant of Anisakis spp. labelled with DOPE-Rho were characterised to assess the effects of labelling on size, structure and fluorescence of EVs. Fluorescent EVs were successfully taken up by the human macrophage cell line THP-1. This study, therefore, presents a novel staining method that can be utilized by the EV field in parasitology and potentially across multiple species.
Silent Speech Decoding Using Spectrogram Features Based on Neuromuscular Activities
Authors: Wang You , Zhang Ming , Wu RuMeng , Gao Han , Yang Meng , Luo Zhiyuan , Li Guang
Published: July 2020

Silent speech decoding is a novel application of the Brain–Computer Interface (BCI) based on articulatory neuromuscular activities, reducing difficulties in data acquirement and processing. In this paper, spatial features and decoders that can be used to recognize the neuromuscular signals are investigated. Surface electromyography (sEMG) data are recorded from human subjects in mimed speech situations. Specifically, we propose to utilize transfer learning and deep learning methods by transforming the sEMG data into spectrograms that contain abundant information in time and frequency domains and are regarded as channel-interactive. For transfer learning, a pre-trained model of Xception on the large image dataset is used for feature generation. Three deep learning methods, Multi-Layer Perception, Convolutional Neural Network and bidirectional Long Short-Term Memory, are then trained using the extracted features and evaluated for recognizing the articulatory muscles’ movements in our word set. The proposed decoders successfully recognized the silent speech and bidirectional Long Short-Term Memory achieved the best accuracy of 90%, outperforming the other two algorithms. Experimental results demonstrate the validity of spectrogram features and deep learning algorithms.
Ictal Asystole in Drug-Resistant Focal Epilepsy: Two Decades of Experience from an Epilepsy Monitoring Unit
Authors: Casciato Sara , Quarato Pier Paolo , Mascia Addolorata , D’Aniello Alfredo , Esposito Vincenzo , Morace Roberta , Pavone Luigi , Di Bonaventura Carlo , Tombini Mario , Assenza Giovanni , Di Gennaro Giancarlo
Published: July 2020

Background: Ictal asystole (IA) is a rare event observed in people with epilepsy (PwE). Clinical and IA video-electroencephalographic findings may be helpful in screening for high-risk subjects. Methods: From all PwE undergoing video-EEG for presurgical evaluation between 2000 and 2019, we retrospectively selected those with at least one IA (R–R interval of ≥3 s during a seizure). Results: IA was detected in eight out of 1088 (0.73%) subjects (mean age: 30 years; mean epilepsy duration: 9.6 years). Four out of them had a history of atonic falls. No patients had cardiac risk factors or cardiovascular diseases. Seizure onset was temporal (n = 5), temporo-parietal (n = 1) or frontal (n = 2), left-sided and right-sided in five and two patients, respectively. In one case a bilateral temporal independent seizure onset was recorded. IA was recorded in 11 out of 18 seizures. Mean IA duration was 13 s while mean IA latency from seizure onset was 26.7 s. Symptoms related to IA were observed in all seizures. Conclusion: IA is a rare and self-limiting event often observed during video-EG in patients with a history of atonic loss of consciousness and/or tardive falls in the course of a typical seizure.
“Mom Let’s Go to the Dentist!” Preliminary Feasibility of a Tailored Dental Intervention for Children with Autism Spectrum Disorder in the Italian Public Health Service
Authors: Narzisi Antonio , Bondioli Mariasole , Pardossi Francesca , Billeci Lucia , Buzzi Maria Claudia , Buzzi Marina , Pinzino Martina , Senette Caterina , Semucci Valentina , Tonacci Alessandro , Uscidda Fabio , Vagelli Benedetta , Giuca Maria Rita , Pelagatti Susanna
Published: July 2020

Children with autism spectrum disorder (ASD) show worse oral health than their peers. Their access to health services is, at present, inadequate: few high-quality interventions have been designed and implemented to improve their care procedures so far. The purpose of this study is to describe an experience of dental care supported by Information and Communication Technologies (ICT), for children with ASD in a public health service. In our study, 59 children (mean age 9.9 years; SD = 5.43) participated in the MyDentist project. It integrates classic dental care techniques with new practices for desensitization and fear control, delivered through an enhanced customized ICT-based intervention aiming at familiarizing the child with ASD with the medical setting and procedures. Two questionnaires were filled out by parents to describe the acceptability of the MyDentist experience for their children. Significant results were shown from T0 (before initiating MyDentist) to T1 (after 6 months of the MyDentist experience) regarding improved oral hygiene and cooperation during dental treatments. Families positively assessed the use of ICT support. In conclusion, the project demonstrated acceptability by parents, suggesting that public health dental care and prevention can be successfully implemented without resorting to costly pharmacological interventions (with potential side effects), taking better care of children’s health.
Contextual-Cueing beyond the Initial Field of View—A Virtual Reality Experiment
Authors: Marek Nico , Pollmann Stefan
Published: July 2020

In visual search, participants can incidentally learn spatial target-distractor configurations, leading to shorter search times for repeated compared to novel configurations. Usually, this is tested within the limited visual field provided by a computer monitor. While contextual cueing is typically investigated on two-dimensional screens, we present for the first time an implementation of a classic contextual cueing task (search for a T-shape among L-shapes) in a three-dimensional virtual environment. This enabled us to test if the typical finding of incidental learning of repeated search configurations, manifested by shorter search times, would hold in a three-dimensional virtual reality (VR) environment. One specific aspect that was tested by combining virtual reality and contextual cueing was if contextual cueing would hold for targets outside the initial field of view (FOV), requiring head movements to be found. In keeping with two-dimensional search studies, reduced search times were observed after the first epoch and remained stable in the remaining experiment. Importantly, comparable search time reductions were observed for targets both within and outside of the initial FOV. The results show that a repeated distractors-only configuration in the initial FOV can guide search for target locations requiring a head movement to be seen.
Role of NAD+—Modulated Mitochondrial Free Radical Generation in Mechanisms of Acute Brain Injury
Authors: Klimova Nina , Fearnow Adam , Kristian Tibor
Published: July 2020

It is commonly accepted that mitochondria represent a major source of free radicals following acute brain injury or during the progression of neurodegenerative diseases. The levels of reactive oxygen species (ROS) in cells are determined by two opposing mechanisms—the one that produces free radicals and the cellular antioxidant system that eliminates ROS. Thus, the balance between the rate of ROS production and the efficiency of the cellular detoxification process determines the levels of harmful reactive oxygen species. Consequently, increase in free radical levels can be a result of higher rates of ROS production or due to the inhibition of the enzymes that participate in the antioxidant mechanisms. The enzymes’ activity can be modulated by post-translational modifications that are commonly altered under pathologic conditions. In this review we will discuss the mechanisms of mitochondrial free radical production following ischemic insult, mechanisms that protect mitochondria against free radical damage, and the impact of post-ischemic nicotinamide adenine mononucleotide (NAD+) catabolism on mitochondrial protein acetylation that affects ROS generation and mitochondrial dynamics. We propose a mechanism of mitochondrial free radical generation due to a compromised mitochondrial antioxidant system caused by intra-mitochondrial NAD+ depletion. Finally, the interplay between different mechanisms of mitochondrial ROS generation and potential therapeutic approaches are reviewed.
Perovskite Solar Cells for BIPV Application: A Review
Authors: Roy Anurag , Ghosh Aritra , Bhandari Shubhranshu , Sundaram Senthilarasu , Mallick Tapas K.
Published: July 2020

The rapid efficiency enhancement of perovskite solar cells (PSCs) make it a promising photovoltaic (PV) research, which has now drawn attention from industries and government organizations to invest for further development of PSC technology. PSC technology continuously develops into new and improved results. However, stability, toxicity, cost, material production and fabrication become the significant factors, which limits the expansion of PSCs. PSCs integration into a building in the form of building-integrated photovoltaic (BIPV) is one of the most holistic approaches to exploit it as a next-generation PV technology. Integration of high efficiency and semi-transparent PSC in BIPV is still not a well-established area. The purpose of this review is to get an overview of the relative scope of PSCs integration in the BIPV sector. This review demonstrates the benevolence of PSCs by stimulating energy conversion and its perspective and gradual evolution in terms of photovoltaic applications to address the challenge of increasing energy demand and their environmental impacts for BIPV adaptation. Understanding the critical impact regarding the materials and devices established portfolio for PSC integration BIPV are also discussed. In addition to highlighting the apparent advantages of using PSCs in terms of their demand, perspective and the limitations, challenges, new strategies of modification and relative scopes are also addressed in this review.
Enhanced Photodegradation of Synthetic Dyes Mediated by Ag3PO4-Based Semiconductors under Visible Light Irradiation
Authors: Pavanello Alice , Blasco Alejandro , Johnston Peter F. , Miranda Miguel A. , Marin Maria Luisa
Published: July 2020

Four silver phosphate-based materials were successfully synthesized, characterized, and evaluated, together with TiO2, in the photodegradation of synthetic dyes (tartrazine, Orange II, rhodamine, and Brilliant Blue FCF) under two irradiation sources centered at 420 and 450 nm. Scanning Electron Microscopy (SEM) images showed different topologies of the synthesized materials, whereas diffuse reflectance spectra demonstrated that they display absorption up to 500 nm. Degradation experiments were performed in parallel with the silver materials and TiO2. Upon irradiation centered at 420 nm, the abatement of the dyes was slightly more efficient in the case of TiO2—except for Orange II. Nevertheless, upon irradiation centered at 450 nm, TiO2 demonstrated complete inefficiency and silver phosphates accomplished the complete abatement of the dyes—except for Brilliant Blue FCF. A careful analysis of the achieved degradation of dyes revealed that the main reaction mechanism involves electron transfer to the photogenerated holes in the valence band of silver photocatalysts, together with the direct excitation of dyes and the subsequent formation of reactive species. The performance of TiO2 was only comparable at the shorter wavelength when hydroxyl radicals could be formed; however, it could not compete under irradiation at 450 nm since the formed superoxide anion is not as reactive as hydroxyl radicals.
Enzymatic Hydrolysis of Softwood Derived Paper Sludge by an In Vitro Recombinant Cellulase Cocktail for the Production of Fermentable Sugars
Authors: Malgas Samkelo , Rose Shaunita H. , van Zyl Willem H. , Pletschke Brett I.
Published: July 2020

Paper sludge is an attractive biomass feedstock for bioconversion to ethanol due to its low cost and the lack of pretreatment required for its bioprocessing. This study assessed the use of a recombinant cellulase cocktail (mono-components: S. cerevisiae-derived PcBGL1B (BGL), TeCel7A (CBHI), ClCel6A (CBHII) and TrCel5A (EGII) mono-component cellulase enzymes) for the efficient saccharification of softwood-derived paper sludge to produce fermentable sugars. The paper sludge mainly contained 74.3% moisture and 89.7% (per dry mass (DM)) glucan with a crystallinity index of 91.5%. The optimal protein ratio for paper sludge hydrolysis was observed at 9.4: 30.2: 30.2: 30.2% for BGL: CBHI: CBHII: EGII. At a protein loading of 7.5 mg/g DW paper sludge, the yield from hydrolysis was approximately 80%, based on glucan, with scanning electron microscopy micrographs indicating a significant alteration in the microfibril size (length reduced from ≥ 2 mm to 93 µm) of the paper sludge. The paper sludge hydrolysis potential of the Opt CelMix (formulated cellulase cocktail) was similar to the commercial Cellic CTec2® and Celluclast® 1.5 L cellulase preparations and better than Viscozyme® L. Low enzyme loadings (15 mg/g paper sludge) of the Opt CelMix and solid loadings ranging between 1 to 10% (w/v) rendered over 80% glucan conversion. The high glucose yields attained on the paper sludge by the low enzyme loading of the Opt CelMix demonstrated the value of enzyme cocktail optimisation on specific substrates for efficient cellulose conversion to fermentable sugars.