bluuebunny/crossref_metadata_2025 huggingface dataset:
Design and Real-Time Control of an Electric Furnace with Three-Dimensional Heating
Authors: Guney Ali , Cakir Oguzhan
Published: February 2025

Currently, heating in electric ovens is generally achieved using heaters placed on the top and bottom surfaces. In some advanced ovens, heaters are installed on the back surface, allowing 3D heating. However, in these ovens, temperature measurements are obtained from a single point, making the temperature control unreliable. Additionally, this type of oven cannot provide homogeneous heating since it lacks heaters on the left, right, or front surfaces. In this study, a unique electric oven equipped with heaters and temperature sensors was designed and produced on all six surfaces. To model its performance, the heating behavior of the oven was derived using the Ziegler–Nichols tangent method, and the gain factors for the proportional (P), proportional-integral (PI), and proportional-integral-derivative (PID) controllers were determined. Subsequently, real-time digital control of the oven was performed using on-off, P, PI, and PID controllers, which ensured a comprehensive evaluation of the oven’s control performance. The experimental results showed that homogeneous heating could only be achieved when all panels were energized. Additionally, the PI and PID controllers stabilized the system with a maximum steady-state error of 1.3 °C in all cases, demonstrating the accuracy of the derived system model and adequacy of the implemented control system.
Design and Analysis of Dual-Band Metasurface Filter for Pulse Waves Based on Capacitive Nonlinear Circuits
Authors: Tian Wenliang , Yang Lingling , Cai Bin , Cheng Yongzhi , Chen Fu , Luo Hui , Li Xiangcheng
Published: February 2025

In this paper, a novel dual-band metasurface filter (MSF) designed for accurately differentiating pulse waves (PWs) and continuous waves (CWs) is proposed, which is based on a complementary cross resonator (CSR) structure adhered on a dielectric substrate integrated with a capacitive nonlinear circuit. The unit cell of the designed dual-band MSF comprises two identical CSR structures: one of the capacitive nonlinear circuits is configured in parallel with a capacitor (C1) within one CSR structure. These structures loaded with nonlinear circuits are fabricated on a dielectric substrate. The simulation outcomes reveal that, for normally incident CWs with an input power of 10 dBm, the transmittance of the designed dual-band MSF reaches as high as 97.1% at 2.0 GHz and 93.9% at 3.45 GHz. In contrast, when it comes to 50 ns short PWs, the transmittance remains consistently below 6% throughout the entire frequency range from 1 GHz to 5 GHz. In addition, the transmittance of the dual-band MSF for normally incident PWs increases significantly as the pulse width widens at the aforementioned two discrete frequencies. The ensuing simulation data corroborates that within the input power range of −15 to 15 dBm, the transmittance difference between CWs and PWs of the dual-band MSF first rises and then falls as the input power increases. Specifically, when the input power is specified as 10 dBm and the angle of oblique incidence ranges from 0° to 60°, in the context of TE and TM modes, the transmittance of CWs exceeds 80% around both 2.0 GHz and 3.45 GHz, while that of PWs remains below 15%. Finally, the effects of resistance and capacitance on the transmittance of the dual-band MSF for the incident PWs and CWs are also studied. The dual-band MSF proposed herein showcases its potential applications in wireless communication as well as in the realm of anti-electromagnetic interference. The electromagnetic (EM) waveform modulation in the frequency band of 1–5 GHz has great development prospects in low-frequency working fields such as radar antennas and EM protection.
Comparison of Methods for Determining the Oil Content in Medium-Mature Shale—A Case Study from the Fourth Member of the Shahejie Formation in the Dongying Depression
Authors: Wang Min , Wang Min , Zhao Xinbin , Li Junliang , Zhang Shun , Tian Min
Published: February 2025

The oil content of shale oil reservoirs is a key parameter for reserve evaluation and “sweet spot” selection. However, discrepancies in the oil contents obtained by different methods have led to considerable disparities in the selection of exploration well locations and development strategies for shale oil. This study focuses on low to moderately mature sealed coring samples from the Dongying Depression in the Bohai Bay Basin. Three methods—NMR, Dean–Stark, and Rock-Eval—were employed to measure the oil content of shale. The results indicate that the oil content obtained by NMR is the highest, followed by the Dean–Stark distillation extraction method, while the pyrolysis method yields the lowest value. The study found that (1) the efficiency of the solvent extraction effect on moderately to low-mature shale is low since the occurrence of numerous closed pores leads to a lower yield when using the Dean–Stark method. (2) The exposure of shale samples to the air, sample crushing, and lag time for temperature increase cause a significant loss of light hydrocarbon components, resulting in the lowest oil content when measured by the Rock-Eval method. The NMR method, with its advantages of a short analysis time and non-destructive nature, appears to be the most advantageous method for shale oil content evaluation.
Prussian Blue Analogues Based on 3d-Metals as Cathode Materials for Magnesium Ion Batteries
Authors: Arbenin Andrey , Egorov Semyon , Prikhodko Igor , Fedorova Anna , Penkova Anastasia , Selyutin Artem
Published: February 2025

Prussian blue PB analogues (K;Mg)xM[Fe(CN)6] were obtained via the co-precipitation method. Manganese, iron, cobalt, nickel, copper and zinc were selected to obtain the metal hexacyanoferrates (MHCFs) of these metals and to systematically study their structural and electrochemical properties as cathode materials. The obtained substances were characterized via X-ray powder analysis, scanning electron microscopy, thermogravimetric analysis and elemental analysis. An electrochemical study of the obtained cathode materials relative to a platinum anode in an aqueous medium and a magnesium anode in a nonaqueous medium was carried out, and the cycling parameters were determined. The influence of 3d-metal nature on the composition–structure–properties of hexacyanoferrates was demonstrated. MHCFs are promising cathode materials for Mg2+ intercalation/deintercalation in aqueous electrolytes.
Economic Optimization of Enhanced Oil Recovery and Carbon Storage Using Mixed Dimethyl Ether-Impure CO2 Solvent in a Heterogeneous Reservoir
Authors: Seo Kwangduk , Kim Bomi , Liu Qingquan , Lee Kun Sang
Published: February 2025

CO2 is the main solvent used in enhanced oil recovery (EOR). However, its low density and viscosity compared to oil cause a decrease in sweep efficiency. Recently, dimethyl ether (DME), which is more efficient than CO2, has been introduced into the process. DME improves oil recovery by reducing minimum miscible pressure (MMP), interfacial tension (IFT), and oil viscosity. Since DME is an expensive solvent, price reduction and appropriate injection scenarios are needed for economic feasibility. In this study, a compositional model was developed to inject DME with impure CO2 streams, where the CO2 was derived from one of these three purification methods: dehydration, double flash, and distillation. It was assumed that such a mixed solvent was injected into a heterogeneous reservoir where gravity override was maximized. As a result, lower oil recovery is achieved for the higher impurity content of the CO2 stream, lower DME content, and more heterogeneous reservoir. When a high-purity CO2 stream is used, the change in oil recovery according to DME content and heterogeneity of the reservoir is increased. When the lowest-purity CO2 stream is used, the net present value (NPV) is the highest. For a homogeneous reservoir, the NPV is highest for all impure CO2 streams. This optimization indicates a greater impact on revenue from reduced CO2 purchase cost than on profit loss due to reduced oil recovery by impurities. Additional benefits can be expected when considering solvent reuse and carbon capture and storage (CCS) credits.
A Novel Optical Instrument for On-Line Measurement of Particle Size Distribution—Application to Clean Coal Technologies
Authors: Cheng Mao , Wargel Zachariah , Magalhaes Duarte , Axelbaum Richard
Published: February 2025

A flow cell is a critical measurement interface for many optical instruments. However, the flows are often sampled under harsh conditions, such as under high pressure and/or high temperature, in the presence of particles, moisture, vapors with high dew points or corrosive gases. Therefore, obtaining a high-optical-quality flow cell that does not perturb the measurement is a significant challenge. To address this challenge, we proposed a new flow cell that employs a unique laminar coaxial flow field (for the purge and sample flows). A test system was built to conduct particle size distribution (PSD) measurements with no sampling bias using a state-of-the-art analyzer (Malvern Panalytical Insitec). The results revealed that the measurement zone is well defined solely by the sample flow, and the optical windows are well protected by the purge flow, with minimal risk of any depositions from the sample flow. Using this flow cell, the Insitec can successfully measure PSD under high pressure and temperature under moist, corrosive conditions without generating any sampling bias. Importantly, we successfully applied this flow cell for on-line PSD measurement for the flue gas of a 100 kWth pressurized oxy-coal combustor operating at 15 bara.
A Bioassay Analysis of Uranium and Lead in Urine Samples from a High Natural Background Radiation Area in Indonesia
Authors: Susanto Very , Pradana Radhia , Nugraha Eka Djatnika , Sumintadireja Prihadi , Modibo Oumar Bobbo , Rosianna Ilsa , Rahajeng Nastiti , Ahmad Haeranah , Kurniawan Rusbani , Rixson Leons , Yuningsih Atika , Siregar Yusraini Dian Inayati , Saepuloh Asep , Wahyudi Wahyudi , Tazoe Hirofumi , Akata Naofumi , Tokonami Shinji
Published: February 2025

Heavy metal pollution is a major environmental concern due to the high toxicity of heavy metals in humans. High natural background radiation areas (HNBRAs) contain high concentrations of the radioactive element 238U, which decays into 206Pb, in their soil, crops, and water. Concentrations of the heavy metals lead (Pb) and uranium (U) are, thus, correlated with HNBRAs. Mamuju in Indonesia is a recently studied HNBRA where high concentrations of Pb and U in the soil have been reported. The present study analyzes Mamuju residents’ exposure to Pb and U. Two zones in the study area were selected for comprehensive assessment. North Botteng was chosen to represent the HNBRA, and Topoyo was selected as the control zone, with 22 urine samples collected from each zone. The samples were analyzed using a quadrupole inductively coupled plasma mass spectrometer (ICP-MS). The average concentrations of Pb measured in the urine samples were 1.31 mg L−1 and 0.77 mg L−1 in North Botteng and Topoyo, respectively. These values are higher than the urine Pb reference value of 5 µg L−1. The urine Pb concentrations in both studied zones were alarmingly high, which may have serious health effects on the population and should warrant action to reduce Pb exposure in this area. The committed effective dose from the ingestion of 238U in North Botteng was higher than in Topoyo, measuring 36.0 mSv and 8.9 mSv, respectively. The area most affected by the ingestion of 238U was the red bone marrow, followed by the bone surface.
Short-Wave Infrared Hyperspectral Image-Based Quality Grading of Dried Laver (Pyropia spp.)
Authors: Lee Jong Bong , Bae Yeon Joo , Kwon Ga Yeon , Sohn Suk Kyung , Lee Hyo Rim , Kim Hyeong Jun , Kim Min Jae , Park Ha Eun , Shim Kil Bo
Published: February 2025

Laver (Pyropia spp.) is a major seaweed that is cultivated and consumed globally. Although quality standards for laver products have been established, traditional physicochemical analyses and sensory evaluations have notable drawbacks regarding rapid-quality inspection. Not all relevant physicochemical quality indices, such as texture, are typically evaluated. Therefore, in this study, we investigated the use of hyperspectral imaging to rapidly, accurately, and objectively determine the quality of dried laver. Hyperspectral images of 25 dried laver samples were captured in the short-wave infrared range from 980 to 2576 nm to assess their moisture, protein content, cutting stress, and other key quality indicators. Spectral signatures were analyzed using partial least-squares discriminant analysis (PLS-DA) to correlate the spectral data with three primary quality index values. The performance of PLS-DA was compared with that of the variable importance in projection score and nonlinear regression analysis methods. The comprehensive quality grading model demonstrated accuracies ranging from 96 to 100%, R2 values from 75 to 92%, and root-mean-square errors from 0.14 to 0.25. These results suggest that the PLS-DA regression model shows great potential for the multivariate analysis of hyperspectral images, serving as an effective quality grading system for dried laver.
Unveiling Photoperiod-Responsive Regulatory Networks in Tropical Maize Through Transcriptome Analysis
Authors: Zheng Tianhui , Bo Jinge , Wang Jing , Li Siyuan , Li Haonan , Liu Mengyao , Niu Hongbin , Nguyen Thanhliem , Chen Yanhui , Sun Juan
Published: February 2025

Background/Objectives: Maize (Zea mays L.), a crop of worldwide importance, owes its adaptability to diverse environments to its genetic variation. However, tropical maize exhibits intrinsic photoperiod sensitivity, limiting its adaptability to temperate regions. Photoperiod sensitivity significantly affects the flowering time and other agronomic traits, but the underlying molecular mechanisms remain poorly understood. In this study, the aim is to elucidate the transcriptional regulatory networks mediating photoperiod responses in tropical maize inbred line Su65, providing insights into improving photoperiod adaptability. Methods: RNA-seq analysis was carried out to investigate photoperiod-responsive genes and pathways in tropical line Su65 exposed to varying photoperiod conditions. Differential expression analysis, functional enrichment, and the construction of protein–protein interaction (PPI) networks were carried out to investigate transcriptional dynamics. Additionally, qRT-PCR was employed to confirm the expression patterns of key candidate genes and generate detailed temporal expression profiles. Results: A total of 1728 differentially expressed genes (DEGs) were identified, with significant enrichment in pathways such as stress responses, redox homeostasis, and secondary metabolite biosynthesis. A set of new key hub genes (such as Zm00001d048531, Zm00001d018821, Zm00001d034892, etc.) were identified through PPI network analysis. Temporal expression profiling of ZmPHYB1, ZmPHYC1, ZmFKF2, ZmGI2, and ZmPRR37a, the key genes involved in circadian rhythms, revealed distinct regulatory patterns of photoperiod-sensitive genes at different time points, highlighting their roles in flowering time regulation and developmental transitions. Conclusions: In this study, critical molecular networks underlying photoperiod sensitivity in tropical maize are uncovered and a foundation is provided for improving photoperiod adaptability through genetic improvement. By integrating RNA-seq and qRT-PCR, the research offers valuable insights into transcriptional dynamics and their role in maize development under photoperiodic regulation.
Maternal and Parent-of-Origin Gene–Environment Effects on the Etiology of Orofacial Clefting
Authors: Rasevic Nikola , Bastasic Joseph , Rubini Michele , Rakesh Mohan R. , Burkett Kelly M. , Ray Debashree , Mossey Peter A. , Peterlin Borut , Khan Mohammad Faisal J. , Ravaei Amin , Autelitano Luca , Meazzini Maria C. , Little Julian , Roy-Gagnon Marie-Hélène
Published: February 2025

Background/Objectives: We investigated maternal and parent-of-origin (PoO) gene-environment interaction effects on the risk of nonsyndromic orofacial clefts for two maternal environmental factors: periconceptional smoking and folic acid supplementation. Methods: Genome-wide single nucleotide polymorphisms (SNPs) genotypes and TopMed-imputed genotypes were obtained for case-parent triads from the EUROCRAN and ITALCLEFT studies. Candidate regions were selected around target SNPs from a previous genome-wide association study, resulting in 12 (726 SNPs) and 11 regions (730 SNPs) for maternal and PoO effects, respectively. Log-linear models were used to analyze 404 case-parent triads and 40 case-parent dyads. p-values were combined across regions. Results: None of the interactions reached statistical significance after correction for the number of regions tested. Nominally significant (pooled p-values < 0.05) interactions pointed to regions in or close to genes LRRC7 (maternal gene-folate interaction), NCKAP5 (PoO-smoking interaction), and IFT43 and GPATCH2L (PoO-folate interaction). Conclusions: Our results suggested that the genetic effects in or around these genes were heightened under periconceptional exposure to tobacco or no folic acid supplementation. The involvement of these genes in orofacial cleft development, in conjunction with environmental exposures, should be further studied.
Adapting Viticulture to Climate Change: Impact of Shading in Sicily
Authors: Miccichè Daniele , Puccio Stefano , Di Lorenzo Rosario , Turano Lucia , Di Carlo Francesco , Pisciotta Antonino
Published: February 2025

Climate change significantly affects viticulture, with noticeable impacts on yield and quality. The increase in average temperatures, often coupled with decreased precipitation, accelerates the phenological development of grapevines, leading to rapid sugar accumulation and concentration and decreased acidity. This study aimed to evaluate the impact of black shading nets with two levels (26% and 40%) on vine phenology, vegetative growth, yield, and grape ripening, as a potential strategy to mitigate the adverse effects of rising temperatures and reduced precipitation. The research was conducted in southwestern Sicily, in the Menfi (AG) area, using Grillo and Syrah grapevines. Black shading nets were applied during the pea-sized berry stage (BBCH 75). The results demonstrated that shading effectively delayed vine phenology and altered grape ripening, with significant reductions in sugar content (up to 10%) and increases in total acidity (up to 10%) at harvest compared to non-shaded vines. However, shading also reduced berry size, resulting in lower cluster weight and yield per plant (up to 15%). These findings highlight the potential of shading nets as a tool for adapting viticulture to climate change, while emphasizing the need to carefully assess their large-scale applicability, considering economic and operational factors.
Millerandage—One of the Grapevine Cultivation Challenges in the Climate Change Context
Authors: Muntean Maria Doinița , Tomoiagă Liliana Lucia , Răcoare Horia Silviu , Sîrbu Alexandra Doina , Giurcă Ioana Sorina , Chedea Veronica Sanda , Teușdea Alin Cristian , Comșa Maria
Published: February 2025

This study investigates millerandage, a physiological disorder affecting grapes during their development. In the climate change context, millerandage can become a viticultural hazard problem causing yield drops and posing challenges regarding wine quality due to uneven ripening in grape clusters. Using the 2023 vintage data from the Research Station for Viticulture and Enology Blaj (SCDVV Blaj), Târnave wine region, Romania, we assessed the climate conditions of 2023, focusing on the adverse climatic conditions from the flowering phenophase and the observed millerandage grade that occurred as a consequence. A total of 26 grapevine cultivars were monitored, assessing millerandage grade by field observations carried out in two grapevine plantations (S1 and S2) in July (BBCH 79) and September (BBCH 87). The results show statistically significant differences, with cultivars like Ezerfurtu (Ez), Napoca (Na), and Rhin Riesling (RR) exhibiting a millerandage grade higher than 35%, while cultivars like Pinot noir (PN) and Pinot gris (PG) showed resilience, with millerandage grades below 1%. These findings highlight cultivar-specific vulnerabilities and provides insights into millerandage’s role as a climate change challenge in viticulture.
Marble Waste Dump Yard in Rajasthan, India Revealed as a Potential Asbestos Exposure Hazard
Authors: Singh Raja , Fitzgerald Sean , Dada Rima , Frank Arthur L.
Published: February 2025

Asbestos is a fibrous variety of certain minerals, some of which occur naturally as an accessory to a wide variety of mineral resources. Although asbestos itself has been historically mined for various useful properties, the negative health effects of asbestos dust have greatly diminished it as a useful earth material, as many countries have banned the use of these fibrous minerals based on those health concerns. Resulting regulations of asbestos have focused primarily on intentionally mined material used in product manufacturing, such as building materials made with beneficiated asbestos and their derivative exposures, e.g., airborne asbestos in schools with asbestos-containing materials. The hazards of asbestos as unintended byproducts have not been as extensively considered, although this “contamination” has been repeatedly observed in common earth materials including talc, vermiculite, sand, and gravel. This study reveals such contamination of ornamental and dimension stone commonly referred to as “marble”. Asbestos types that can be associated with certain Indian marble reserves include asbestiform tremolite, actinolite, anthophyllite, and chrysotile asbestos. This case reveals such contamination in a marble reserve in Rajsamand, Rajasthan. At this location, marble dust in slurry is disposed at waste collection points, unfortunately including a location now open to the public that has become a tourist destination. Using Transmission Electron Microscopy (TEM) in this study, dust from this location revealed abundant tremolite asbestos fibres in the disaggregated dust. This poses potential health risks to the workers, bystanders, and tourists that may be exposed to this recognized carcinogen, a known cause of mesothelioma, lung cancer, and other asbestos-related diseases.
Proteomic Analysis of Midgut of Silkworm Reared on Artificial Diet and Mulberry Leaves and Functional Study of Three UGT Genes
Authors: Zhang Shengxiang , Zhang Xinran , Liang Jiawen , Huang Shuxian , Huang Bokai , Ren Chunjiu , Gao Huiju , Liu Qingxin
Published: February 2025

There remains a significant gap in production performance and disease resistance between silkworms reared on artificial diets and those reared on mulberry leaves. This study aims to identify key differential proteins through proteomic analysis of the midgut of silkworms fed artificial diets compared to those fed mulberry leaves. Utilizing molecular docking technology, three anti-nutritional factors that consistently bind to the UGT40B4, UGT340C2, and UGT40A1 proteins were selected, and the differential expression of these UGT genes in response to various anti-nutritional factors was examined. The findings indicate that variations in feed significantly influence the expression of digestive, metabolic, and immune-related proteins within the silkworm midgut. Notably, the expression levels of the UGT40B4, UGT340C2, and UGT40A1 genes vary across different silkworm organs and developmental stages, reflecting their respective physiological roles. Furthermore, the effects of soybean isoflavone, tannic acid, and arabinoxylan on silkworm growth and cocoon quality were found to differ when these substances were incorporated into semi-synthetic feed. This research is anticipated to provide valuable insights for future studies on the role of UGT genes in the silkworm midgut and the formulation of artificial diets for silkworms.
The Lower Concentration of Plasma Acetyl-Carnitine in Epicardial Artery Disease—A Preliminary Report
Authors: Urbanowicz Tomasz , Gutaj Paweł , Plewa Szymon , Olasińska-Wiśniewska Anna , Spasenenko Ievgen , Krasińska Beata , Tykarski Andrzej , Filipiak Krzysztof J. , Pakuła-Iwańska Martyna , Krasiński Zbigniew , Grywalska Ewelina , Wender-Ożegowska Ewa , Jemielity Marek , Matysiak Jan
Published: February 2025

Coronary artery disease remains an epidemiological challenge as global morbidity is not declining despite the fact that the risk factors are well known. Metabolomic derivatives of atherosclerosis formation have recently gained attention as a possible non-traditional risk factor. The aim of this study was to find potential differences in acetyl-carnitine chain serum concentrations between epicardial artery disease patients and a control group. There were 41 patients (25 men and 16 women), with a median (Q1–Q3) age of 69 (63–73) years, enrolled in the prospective metabolomic analysis. They were divided into two groups based on cine angiography results confirming epicardial artery disease (group 1, n = 25 (61%)) or showing characteristics corresponding to normal angiograms (group 2, n = 16 (39%)). The quantitation of metabolites was performed based on the coronary angiograms. Significant differences related to the plasma concentration of L-Acetyl-carnitine (7.49 (4.79–9.23) µM vs. 9.36 (8.57–10.23) µM (p = 0.009)), Decanoyl-carnitine (0.00 (0.00–0.37) µM vs. 0.36 (0.19–0.44) µM (p = 0.040)), C12:1-carnitine (0.17 (0.14–0.20) µM vs. 0.22 (0.18–0.24) µM (p = 0.008)), trans-2-Dodecenoyl-carnitine (0.10 (0.07–0.13) µM vs. 0.13 (0.10–0.15) µM (p = 0.002)), cis-5-Tetradecenoyl-carnitine (0.03 (0.02–0.04) µM vs. 0.04 (0.03–0.05) µM (p = 0.043)), and 3,5-Tetradecadien-carnitine (0.16 (0.14–0.18) µM vs. 0.18 (0.17–0.27) µM (p = 0.007)) in group 1 vs. group 2 were noted. Increased plasma levels of acetyl-carnitine may be characteristic of patients with normal coronary angiograms.
Exploring the Role of Lower Genital Tract Microbiota and Cervical–Endometrial Immune Metabolome in Unknown Genesis of Recurrent Pregnancy Loss
Authors: Mikhalev Sergey A. , Kurtser Mark A. , Radzinsky Victor E. , Orazov Mekan R. , Beeraka Narasimha M. , Mikhaleva Lyudmila M.
Published: February 2025

Recurrent pregnancy loss (RPL) of unknown genesis is a complex condition with multifactorial origins, including genetic, hormonal, and immunological factors. However, the specific mechanisms underlying endocervical cell proliferation disorders in women with RPL remain inadequately understood, particularly concerning the role of microbiota and viral infections. The aim of this study was to investigate the mechanisms of endocervical cell proliferation disorders in women with RPL of unknown genesis by examining microbiota, human papillomavirus (HPV) typing, and the expression levels of key molecular biological markers, including p16/Ki-67, BCL-2, miR-145, and miR-34a. A prospective observational comparative study was executed on women with RPL and healthy pregnant controls with full ethical approval. Samples were collected for HPV typing and immunocytochemical analysis to evaluate the expression of p16, Ki-67, BCL-2, and the anti-oncogenic microRNAs (miR-145 and miR-34a). The expression of mRNA for the progesterone receptor (PGR-A) was also assessed, alongside local immune status markers, including proinflammatory T-lymphocytes (Th17/Th1) and regulatory CD4+ Tregs. Overexpression of p16, Ki-67, and BCL-2 was observed in 52.5% of women with RPL who had an ASC-US/LSIL cytogram, with the average double expression of p16/Ki-67 being three times higher than in the healthy pregnant group. A significant decrease in PGR-A mRNA expression in the endocervix of women with RPL was noted, accompanied by a dysregulated local immune status characterized by an increased prevalence of Th17/Th1 cells and a reduction in regulatory CD4+ Tregs. Additionally, the expression of miR-145 and miR-34a in the endocervix and endometrium of women with RPL significantly differed from the physiological pregnancy group, particularly in the context of high-risk HPV infection. The findings describe that disorders of endocervical cell proliferation in women with RPL of unknown genesis are associated with overexpression of specific molecular markers, impaired immune regulation, and altered microRNA profiles. These alterations may contribute to the pathophysiology of RPL, highlighting the need for further research into targeted interventions that could improve reproductive outcomes in affected individuals.
Gut Bacteria-Derived Tryptamine Ameliorates Diet-Induced Obesity and Insulin Resistance in Mice
Authors: Lee Jongjun , Jang Hye-Rim , Lee Dongjin , Lee Yeonmi , Lee Hui-Young
Published: February 2025

Tryptophan is an essential amino acid that is metabolized in the intestine by gut bacteria into indole derivatives, including tryptamine. However, little is known about which bacterial tryptophan metabolites directly influence obesity. In this study, we identified tryptamine as a bacterial metabolite that significantly reduced fat mass following the intraperitoneal injection of five bacterial tryptophan end-products in a diet-induced obese mouse model. Interestingly, tryptamine, a serotonin analog, inhibited both lipogenesis and lipolysis in adipose tissue, which was further confirmed in a 3T3-L1 adipocyte cell culture study. Moreover, oral tryptamine supplementation markedly reduced fat mass and improved insulin sensitivity in a long-term, high-fat-diet, pair-feeding model. These studies demonstrate the therapeutic potential of tryptamine, a bacterial tryptophan metabolite, in ameliorating obesity and insulin resistance by directly regulating lipogenesis and lipolysis in white adipose tissue.
A Maize Calmodulin-like 3 Gene Positively Regulates Drought Tolerance in Maize and Arabidopsis
Authors: Li Dan , Wang Hanqiao , Luo Fushun , Li Mingrui , Wu Zhiqiang , Liu Meiyi , Wang Zhen , Zang Zhenyuan , Jiang Liangyu
Published: February 2025

Drought stress is one of the important abiotic stresses that affects maize production. As an important Ca2+ sensor, calmodulin-like proteins (CMLs) play key roles in plant growth, development, and stress response, but there are a limited number of studies regarding CMLs in response to drought stress. In this study, a Calmodulin-like gene, namely ZmCML3, was isolated from maize (Zea mays L.). The coding sequence (CDS) of ZmCML3 was 474 bp and a protein of 158 aa which contains three EF-hand motifs. ZmCML3 was localized within the nucleus and plasma membrane. The expression of ZmCML3 was induced by polyethylene glycol (PEG) 6000, NaCl, methyl jasmonate (MeJA), and abscisic acid (ABA). Overexpression of ZmCML3 resulted in enhanced drought tolerance in maize through increasing proline (Pro) content and the activity of peroxide (POD) and superoxide dismutase (SOD). Meanwhile, ZmCML3 also positively regulated the expression of drought stress-responsive genes in maize under drought stress treatment. Taken together, ZmCML3 acts as a positive regulator in maize response to drought stress. These results will provide theoretical basis for breeding drought tolerance maize variety.
Oviposition Deterrents from Extracts of Eryngium foetidum Against Potato Tuber Moth Phthorimaea operculella Zeller (Lepidoptera: Gelechiidae)
Authors: Ma Yanfen , Yang Xinzhou , Wu Mei , Guo Yunjiao , Dong Wenxia , Tang Rui , Xiao Chun
Published: February 2025

The potato tuber moth Phthorimaea operculella is a serious boring pest of potato. An integrated ecological approach to sustainable pest management is necessary for the control of this species. This study investigated the effects of minced leaves and plant extracts of Eryngium foetidum on the oviposition behavior of PTM females. The behavioral regulatory components of PTM females in response to the extracts were determined using chemical analyses and electrophysiological tests. Individual electroantennographic detection (EAD)-active compounds and mixtures were evaluated using oviposition choice bioassays. The results indicate that minced leaves had a deterrent effect on oviposition. The extracts at low dosages had an attraction effect, while high dosages had a repellent effect on the oviposition of PTM adult females. The dominant compounds of the extracts were trans-2-dodecenal and trans-2-tridecenal and showed EAD activity. trans-2-dodecenal, trans-2-tridecenal and their mixtures showed significant oviposition-repellent effects toward the PTM. The oviposition stimulation indices (OSIs) of trans-2-dodecenal and trans-2-tridecenal were −100% and −94.03% at 10 mg/mL, respectively. The OSIs of mixtures at natural ratios of 10 mg/mL and 5 mg/mL were −95.11% and −90.96%, respectively. The results can be used for the further development of ecological control strategies for this pest species.
Bone Densitometry Parameters in Females with Ehlers-Danlos Syndrome—Does the Hypermobile Subtype Increase the Risk of Low Bone Mass in Patients with Ehlers-Danlos Syndrome?
Authors: Kałuża Bernadetta , Rychlik Ivan , Domański Jan , Żuk-Łapan Aleksandra , Babula Emilia , Poprawa Iga , Podstawka Jakub , Kowalów Ewa , Franek Edward
Published: February 2025

Background: The purpose of this study was to assess bone densitometry parameters in patients with classical and hypermobile Ehlers-Danlos syndrome (EDS) and to determine whether the hypermobile subtype increases the risk of low bone mass, which is particularly important in this patient group, since the genetic mutation responsible for this subtype is still unknown. Material and Methods: In order to conduct this study, we collaborated with the EDS society in Poland. A total of 30 females of reproductive age who were included in the study were divided into two groups: Group 1—those with classical EDS (n = 9) and Group 2—those with hypermobile EDS (n = 21). Routine laboratory test results, bone turnover markers, and densitometry parameters were evaluated and compared. Results: The study groups showed no differences in terms of densitometry parameters or markers of calcium-phosphate metabolism. A multivariate logistic analysis demonstrated no increase in the risk of low bone mass (defined as a Z-score lower than −2) in patients with hypermobile EDS (OR 0.067 [95% Cl 0.0–20.927]; p = 0.356). Conclusions: The hypermobile subtype of EDS does not increase the risk of low bone mass; there were no significant differences between patients with hypermobile EDS and those with classical EDS in terms of either densitometry parameters or markers of calcium-phosphate metabolism. Although patients with hypermobile EDS are not at a higher risk of developing low bone mineral density, they should be regularly monitored for any calcium and phosphate metabolism abnormalities.
Pancreatitis Risk Associated with GLP-1 Receptor Agonists, Considered as a Single Class, in a Comorbidity-Free Subgroup of Type 2 Diabetes Patients in the United States: A Propensity Score-Matched Analysis
Authors: Ayoub Mark , Chela Harleen , Amin Nisar , Hunter Roberta , Anwar Javaria , Tahan Veysel , Daglilar Ebubekir
Published: February 2025

Introduction: Glucagon-like peptide-1 receptor agonists (GLP-1 RAs) are commonly prescribed for the management of type 2 diabetes mellitus (T2DM). However, the potential connection between GLP-1 RAs and the risk of pancreatitis presents a complex and nuanced issue. Although these drugs are effective in improving blood sugar control and cardiovascular health, their association with pancreatitis remains an area of concern. Our study aims to evaluate the association between the use of GLP-1 RAs, considered as a single class, and the risk of pancreatitis in a comorbidity-free subgroup of patients with type 2 diabetes mellitus (T2DM) in the United States. Methods: Data were retrieved from the TriNetX research database using the US Collaborative Network, which included information from 61 healthcare organizations within the U.S. Patients diagnosed with T2DM were categorized into two cohorts: one consisting of the patients prescribed with GLP-1 RAs and the other comprising patients who did not receive GLP-1 RAs. Of this class of medications, the agents analyzed were dulaglutide, lixisenatide, exenatide, liraglutide, and semaglutide. Using a 1:1 propensity score matching (PSM) model, we matched patients of both cohorts based on baseline demographics, comorbidities (hypertensive disorders, ischemic heart disease, gallstones, annular pancreas, alcohol use disorders, hypertriglyceridemia, hypercalcemia, cystic fibrosis, and cannabis use), medications known to cause drug-related pancreatitis, and laboratory values. Results: Of 969,240 patients with T2DM, 9.7% (93,608) were on GLP-1 RA, and 90.3% (875,632) were not. After PSM, the sample included 81,872 patients in each cohort. The risk of pancreatitis between the two groups was not statistically different between the two cohorts at 6 months at (0.1% vs. 0.1%, p = 0.04), and remained without significant increase with time; at 1 year (0.1% vs. 0.2%, p = 0.02), 3 years (0.2% vs. 0.3%, p = 0.001), and 5 years (0.3% vs. 0.4%, p < 0.001). The lifetime risk of developing pancreatitis in patients on GLP-1 RA was lower (0.3% vs. 0.4%, p < 0.001). Conclusions: In our comorbidity-free U.S.-based population with T2DM, the use of GLP-1 RAs did not increase their risk of pancreatitis. Their use was associated with a lower lifetime risk of pancreatitis.
Autoimmunity in Cardiomyopathy-Induced Heart Failure and Cardiac Autoantibody Removal by Immunoadsorption
Authors: Dandel Michael
Published: February 2025

There is increasing evidence that β1-adrenoreceptor autoantibody (β1AR-AAb) elimination can break the vicious circle induced by certain pathological conditions associated with alteration of the physiological self-tolerance, followed by generation of such AAbs and activation of cell-mediated immune processes directed against the myocardium. Concerning this, the present narrative review article provides an updated overview of the state of knowledge about the role of auto-immunity in the etiopathogenesis of cardiomyopathies, with a particular focus on immunoadsorption (IA) therapy for β1AR-AAb-positive adult patients with a dilated cardiomyopathy (DCM)-associated refractory heart failure (HF). Among many relevant findings, the increasing prevalence (up to 97%) of β1AR-AAb-positive patients related to the aggravation of HF, the high prevalence (between 84% and 91%) of HF patients in which IA can reduce to a minimum any increased β1AR-AAb level, as well as the high prevalence (about 80%) of responders to the IA-induced normalization of β1AR-AAb levels by long-term improvement in LV ejection fraction with increase in LV stroke volume and cardiac output, are of particular relevance. Given that after the elimination of β1AR-AAbs in potential candidates for heart transplantation (HTx), the post-IA 3- and 5-year HTx-/mechanical support-free survival probability reached 80% and 63-69%, respectively, the good tolerability of IA and the possibility to repeat that therapy also in elderly persons strongly suggest that in appropriately selected patients, this therapy deserves much more attention in the future.
Intuitive and Minimally Invasive Surgical Technique for Comminuted Mid-Shaft Clavicle Fractures: Fixation with an Anterior Mini Plate and Superior Locking Compression Plate
Authors: Ok Seungwoo , Yoon Seong-Meen , Choi Sungwook
Published: February 2025

Background: We have applied an anterior locking compression mini plate in addition to a conventional superior locking compression plate for the treatment of wedge or multifragmentary clavicular fractures. Methods: Medical and radiologic data were retrospectively reviewed for patients who underwent surgical fixation with an anterior locking compression mini plate and conventional anatomical locking compression plate in a clavicle fracture of AO/OTA 15.2 B and 15.2 C. The primary outcome was bone union, and the secondary outcome was postoperative complications associated with the procedure. The functional outcomes included the Visual Analog Scale (VAS), University of California at Los Angeles Shoulder Scale (UCLASS), and Constant Shoulder Scale (CSS). Results: Nineteen patients with AO/OTA 15.2 B and 2 C clavicular fractures were followed for an average of 16 months (range: 12–30). The average patient age was 41 (range: 21–76) years, and 17 male and 2 female patients were included. The most common cause of clavicle fractures was sports activity (36.8%). A total of 10 patients had AO/OTA classification 15.2 C, and 9 patients had AO/OTA classification 15.2 B clavicular fractures. Primary fracture union healing was observed in all 19 (100%) patients, and the average time to bone union was 11 weeks (range: 7~21). There was no fixation failure or postoperative infection. The mean VAS, UCLASS, and CSS scores at the final follow-up were 0.6, 33.4, and 65 on a 75-point scale (87 on a 100-point scale), respectively. Conclusions: Dual plating using an anterior mini plate with a superior LCP could be considered as an option to minimize soft-tissue injury in comminuted mid-shaft clavicle fractures.
A Prediction of Estuary Wetland Vegetation with Satellite Images
Authors: Yang Min , Guo Bin , Gao Ning , Yu Yang , Song Xiaoli , Gu Yanfeng
Published: February 2025

Estuarine wetlands are the transition zone between marine, freshwater, and terrestrial ecosystems and are more ecologically fragile. In recent years, the spread of exotic vegetation, specifically Spartina alterniflora, in the Yellow River estuary wetlands has significantly encroached upon the habitats of native species such as Phragmites australis, Suaeda glauca Bunge, and Tamarix chinensis Lour. With advances in land prediction modeling, predicting wetland vegetation distribution can aid management and decision-making for ecological restoration. We selected the core area as the study object and coupled the hydrological model MIKE 21 with the PLUS model to predict the potential future distribution of invasive and dominant species in the region. (1) Based on the fine classification results from satellite images of GF1/G2/G5, we gained an understanding of the changes in wetland vegetation types in the core area of the reserve in 2018 and 2020. (2) Using public data such as ERA5 and GEO as input for basic environmental data, using MIKE 21 to provide high-spatial-resolution hydrodynamic parameters for the PLUS model as an environmental driver, we modeled the spatial distribution of various wetland vegetation in the Yellow River estuary wetland in Dongying under different artificial restoration measures. (3) We predicted the 2022 distribution of typical vegetation in the region, used the classification results of GF6 as the actual distribution, compared the spatial distribution with the actual distribution, and obtained a kappa coefficient of 0.78; the predicted values of the model are highly consistent with the true values. This study combines the fine classification results of vegetation based on hyperspectral remote sensing, the construction of a coupled model, and the prediction effect of typical species, providing a reference for constructing and optimizing the vegetation prediction model of estuarine wetlands. It also allows scientific and effective decision-making for the management of ecological restoration of delta wetlands.
Half-Century Scientometric Analysis: Unveiling the Excellence of Fungi as Biocontrol Agents and Biofertilisers
Authors: Yuan Ziqi , Shen Qi , Yu Kefei , Liu Yan , Zheng Huabao , Yao Yanlai , Jia Baolei
Published: February 2025

Reducing the use of chemical inputs is becoming a major challenge in developing sustainable agriculture. Fungi, known as biocontrol agents (BCAs) and biofertilisers, are crucial in scientific research and are celebrated for their efficacy, eco-friendliness, and multifaceted roles. In this study, a bibliometric analysis was conducted on 5349 articles related to fungi as BCAs and biofertilisers over the past half-century using the Web of Science Core Collection (WoSCC) database. The publications on fungi, such as BCAs and biofertilisers, have increased significantly over the last 20 years, with a maximum growth rate of 33.7%. The USA and China lead in this field. Keyword clustering analysis revealed that entomopathogenic fungi, including Hemiptera, Coleoptera, and Lepidoptera, can be used to manage plant pests. It also showed that fungi can be used as biofertilisers to promote plant growth. The analysis of research trends shows that Beauveria bassiana in biological control is highly significant. This study also showed that entomopathogenic fungi control plant pests by infiltrating the insect cuticles. Trichoderma spp. exert biocontrol effects by producing antibiotics. Arbuscular mycorrhizal fungi can trigger plant defence mechanisms by modulating secondary metabolite synthesis. This study contributes to the current knowledge of fungi as BCAs and biofertilisers and can guide future research.
Landscape Pattern Changes and Ecological Vulnerability Assessment in Mountainous Regions: A Multi-Scale Analysis of Heishui County, Southwest China
Authors: Gu Weiqi , Fu Hong , Jin Wen
Published: February 2025

Against the backdrop of intensifying global climate change, the impact of land use pattern changes on ecosystem vulnerability has garnered increasing attention. However, systematic studies concerning the ecological vulnerability of mountainous regions remains inadequate, with relevant policies primarily remaining at the macro-regulation level and lacking specific guidance measures. Taking Heishui County in southwest China as a case study, this research innovatively combines landscape pattern with the sensitivity–pressure–resilience (SPR) model to systematically analyze land use spatiotemporal evolution characteristics, ecological vulnerability and spatial differentiation patterns, and their driving mechanisms across multiple scales, including county, township, and land use types. The findings reveal that the region’s ecological vulnerability exhibits a spatial distribution pattern of “high in the southeast and low in the northwest”, with a radiating decrease from the centers of Longba and Weigu towns. The high degree of farmland landscape fragmentation is identified as a crucial factor contributing to its heightened ecological vulnerability. Geological disasters, human activities, meteorological conditions, and topographical features are the primary driving factors affecting ecological vulnerability intensity. Furthermore, land use pattern changes, characterized by landscape patch fragmentation, lack of dominant patch types, and decreased landscape diversity have further intensified regional ecological vulnerability. This research holds significant theoretical and practical implications for guiding ecological environmental governance in mountainous regions, enhancing ecological resilience, and promoting regional sustainable development.