bluuebunny/crossref_metadata_2025 huggingface dataset:
Frecuencia de terceros molares retenidos con relación al biotipo facial. un estudio transversal
Authors: Zeta Rodríguez Taylor Augusto
Published: June 2022

Objetivo: Identificar la frecuencia de terceros molares retenidos con relacion al biotipo facial utilizando estudios 2D (panoramicos y cefalometricos) de pacientes que acudieron al centro de imagenes Zeta-Exmed en el periodo de noviembre de 2018 a diciembre de 2021, en la ciudad de Lima, Peru. Metodologia: El tipo de estudio de esta investigacion fue observacional, descriptivo, transversal y retrospectivo. Se evaluaron 6000 casos, cada uno compuesto de una imagen radiografica panoramica y una cefalometrica, ambas tomadas al paciente en la misma fecha, de las cuales solo fueron incluidos en la investigacion un total de 150 casos, por cumplir con los criterios de seleccion. Las mediciones sobre la frecuencia de terceros molares retenidos con relacion al biotipo facial se realizaron por un investigador entrenado y calibrado, utilizando el software Nemoceph para los trazados cefalometricos. Se utilizo para el analisis estadistico el programa SPSS version 1.0.0.1401 (SPSS Inc., EE. UU.) y la prueba chi cuadrado (p < 0,05). Resultados: De los 150 casos revisados, 91 correspondieron al sexo femenino (60,66%) y 59 al sexo masculino (39,33%), lo que dio como resultado que el biotipo dolicofacial se presento con mayor frecuencia en estos, con un total de 27 casos (45,80%), mientras que, en mujeres, el biotipo braquifacial fue el de mayor frecuencia, con un total de 35 casos (38,50%). Asimismo, del total de terceros molares retenidos evaluados (403 piezas), 141 se presentaron en casos con biotipo dolicofacial, seguido del biotipo mesofacial con 139 piezas y, finalmente, el biotipo braquifacial con 123 piezas. Respecto de la evaluacion de los terceros molares retenidos en relacion con su posición y biotipo facial, se encontro 63 3MR en posicion mesioangular y 28 en posicion distoangular en dolicofacial, seguidos de 48 piezas en posicion vertical en braquifacial. Conclusiones: No existe una relacion significativa entre la cantidad de terceros molares retenidos y su posicion, en relacion con el biotipo facial, pero si respecto a la relacion entre el sexo y el biotipo facial, pues los varones presentan un biotipo dolicofacial con mayor frecuencia y las mujeres el biotipo braquifacial.
A Clinical Study of Liposuction combined with Lymphovenous Anastomosis for Treatment of Breast Cancer-Related Lymphedema
Authors: Chang Kun , Xia Song , Liang Chen , Sun Yuguang , Xin Jianfeng , Shen Wenbin
Published: June 2022

Objective
In this work, we studied the clinical effect of liposuction combined with lymphovenous Anastomosis (LVAs) for treatment of breast cancer-related lymphedema (BCRL).
Methods
We studied 158 patients with unilateral upper limb BCRL underwent liposuction combined with LVAs. Arm circumferences before and 7 days after the combined treatments were prospectively recorded. Circumferences of different upper extremities were measured before the procedure, 7 days after LVAs and during the follow-ups. Volumes were calculated with the frustum method. During the follow-ups, the conditions of patients’ treated arms, i.e. the frequency of erysipelas episodes and dependence on compression garments, were recorded.
Results
The mean circumference difference between two upper limbs decreased significantly from M (P25, P75) 5.3 (4.1, 6.9) preoperatively to 0.5 (-0.8, 1.0) (P < 0.01)7 days after treatments, while at follow-up 0.3 (-0.4,1.0). The mean volume difference decreased significantly from M (P25, P75) 838.3(662.4, 1129.0)preoperatively to 7.8 (-120.3, 151.4) (P < 0.01)7 days after treatments, while at follow-up 43.7 (-59.4,161.1). The incidence of erysipelas also significantly decreased (P < 0.01). 6.3% patients were already independent of compression garments during the past six months or even more.
Conclusion
Liposuction combined with LVAs is an effective method for the treatment of BCRL.
Concoction of CAPE and Crocin augments anti-cancer potential and realigns the expression paradigm of p53, EGFR and PCNA in U87-MG Glioma cells
Authors: Mir Ashaq Hussain , Mir Khurshid Iqbal , Banday Mujeeb Zafar , Balkhi Henah Mehraj , Haq Ehtishamul
Published: June 2022

Objective: An adjunct is expected to be identified through this study that will be used alone or in combination with the existing therapeutic regimen for the treatment of gliomas. Results: CAPE and Crocin when used in a congruous combination and durations, present an anticancer potential for glioma. CAPE and Crocin in combination have been shown to inhibit proliferation and induce apoptosis in U87-MG glioma cells. Our results provide evidence that combination treatment realign the expression paradigm of p53, EGFR and PCNA in co-treated U87-MG cells. Compared to efficacy of a single drug, combination of CAPE and Crocin has revealed significant antineoplastic effectiveness in treating gliomas. Together, co-treatment augments effectiveness and halt glioma invasion and proliferation significantly. This implies that the treatment of glioma with combination of the drugs could be considered in prospective therapeutic algorithm for the curative treatment of gliomas.
Effect of wearing medical protective masks on treadmill running performance in the postpandemic era: a randomised trial
Authors: Wang I-Lin , Su Yu , Yao Shun , Wu Chou , Lai Chien-Ying
Published: June 2022

In the postpandemic era, wearing protective masks in public places will still be an important means of blocking popular viruses in the future. (1) Background: The purpose of this study was to explore whether sports performance was affected by mask wearing and exercise duration during 15-minute treadmill running at a speed of 75% maximal aerobic speed. (2) Methods: Thirty-six males were randomly divided into mask and nonmask groups. The kinematic and kinetic data were obtained at four time points (RN0-1 min, RN5-6 min, RN9-10 min, and RN14-15 min) after running. Two-way repeated-measures ANOVA was applied to examine the effects between groups and times with Bonferroni post hoc comparison. (3) Results: The results showed that there was no difference between mask and nonmask group during running (p > 0.05). As running time increased, hip joint ROM, hip joint flexion/extension max, and ankle joint plantarflexion max angles increased; knee joint flexion min and ankle joint dorsiflexion max angles decreased; average peak vertical ground reaction forces (PVGRF) increased after 9 min-running (p < 0.05). (4) Conclusions: Wearing a medical protective mask does not affect the joint angle and touchdown PVGRF of lower extremities during treadmill running while affected by running time and changed after 9 min-treadmill running. Future studies will examine the effects of wearing masks during the pandemic on muscle activation and blood biochemical values during exercise. The trial was registered prospectively with trial registration number (TRN) of ChiCTR2000040535 and date of registration in December 1, 2020.
Aerobic exercise alleviates ventilator-induced lung injury due to activation of NLRP3 inflammasome
Authors: Liu Mengjie , Yan Jie , Wang Yuelan
Published: June 2022

BackgroundVentilator-induced lung injury (VILI) is a type of lung injury caused by stretch stimulation and other reasons for mechanical ventilation. NOD-like receptor protein 3(NLRP3) is an important ingredient of innate immunity and is strongly associated with VILI. This paper aims to investigate the effect of aerobic exercise on VILI and the role of NLRP3.MethodsC57BL/6 male mice (7-week-old, 19~23g) were pre‐treated with Bisindolylmaleimide I (specific and highly efficient inhibitor of PKC) . Then the mice were subjected to mechanical ventilation at a high tidal volume. For another study, the mice were randomly divided into the following four groups:control group (C group), mechanical ventilation group (MV group), exercise group (EX group) and exercise + mechanical ventilation group (EX+MV group) which were subjected to mechanical ventilation or 5 weeks of aerobic exercise training. At the end of ventilation, hematoxylin-eosin (HE) staining and wet/dry weight ratio were used to access the pathophysiological changes of lung tissues. The expression of PKCɑ, P-PKCɑ,ASC,Procaspase-1,caspase-1,ProIL-1β,IL-1β,NLRP3 and tight junction protein occludin in lung tissues were determined by Western blot. The contents of IL-6 in alveolar lavage fluid were determined by ELISA.ResultsMechanical ventilation could increase the levels of NLRP3, P-PKCɑ, PKCɑ, while these changes could be reversed by Bisindolylmaleimide I. Inhibited the PKC production can prevented the activation of NLRP3. Moreover, mechanical ventilation also could increase the levels of ASC,Procaspase-1,caspase-1,ProIL-1β,IL1β and decrease the levels of occludin, while these changes could be alleviated by aerobic exercise. HE staining and lung injury scores all confirmed that no obvious lung injury to group C and EX, while the severity of lung injury was the severest in group MV but was improved on group EX+MV.Taken together, these findings suggest that mechanical ventilation activated the NLRP3 inflammasome by activating PKCɑ, induced the degradation of occludin; while aerobic exercise attenuated activation of NLRP3 inflammasome and PKCɑ and mitigated the decrease in occludin expression caused by mechanical stretch.ConclusionActivation of NLRP3 leads to acute lung injury. Aerobic exercise can reduce lung injury by inhibiting activation of PKCɑ and NLRP3, suggesting a potential preventive measure for the clinical treatment of VILI.
Performance Comparison of Various EBG Structures
Authors: latha prema , Gammadi srikanth , Rangasai Kiriti
Published: June 2022

This paper mainly deals with performance comparison of various EBG antennas for WIFI applications.The proposed designshave a size of 28.11x32.40x1.59 mm3with RT / Duroid 5880 substrate having a thickness of 1.59mm and Ɛr = 2.2. In recent years the use of electromagnetic band gap structures, has grown rapidly in the antenna designs due to its unique properties. EBG structures are used in the antenna to enhance its performance characteristics. In this paper variousEBG antennasare designed and replicated using HFSS software. Various EBGs like Fork, Fractal, Spiral are placed on Ground plane and on the patch. Performance parameters such as return loss, radiation patterns and Gain are measured and are reported.
Clinical analysis of risk factors of acquired immunodeficiency syndrome complicated with pulmonary tuberculosis
Authors: She Li-jun , Zhang Xin , Liu Shi-fu , Ma Jian-hui , Cao Jin-feng
Published: June 2022

Objective
To analyze the risk factors of acquired immunodeficiency syndrome(AIDS)complicated with pulmonary tuberculosis༈PTB༉, so as to provide a reference for better treatment and prevention .
Methods
A retrospective analysis of 939 AIDS patients admitted to our hospital from January 2016 to January 2021 was divided into AIDS combined PTB group (group A, 68 cases) and AIDS group (group B, 871 cases) according to whether they were complicated with PTB. The clinical and laboratory data were analyzed and compared between the two groups.
Results
There were no significant differences between the two groups in gender, residence, occupation, annual income, educational level, marital status, drinking history, history of diabetes, history of BCG vaccination, homosexuality, and whether there were multiple sexual partners at the same time (P > 0.05). ). There were significant differences in smoking history and the number of CD4 + cells (200 cells/mm3 as the boundary), with χ2 = 8.98 and χ2 = 9.65, respectively, P < 0.05.
Conclusion
Smoking history and CD4 + cell count < 200 cells/mm3 were risk factors for AIDS complicated with PTB.
BYSL: A Novel Biomarker for Cancer Treatment
Authors: Li Yuqing , Li Kexin , Zou Jiahua , An Ran , Xu Fang , Liu Zhong
Published: June 2022

Background: BYSL, Bystin like, was previously characterized as an important gene for cell proliferation, migration, invasion, and mesenchymal transformation in human carcinoma. However, systematic pan-cancer analysis of BYSL has never been developed to comprehend its regulation in cancer so far.Methods: The potential carcinogenic role of BYSL in 33 carcinomas based on the Cancer Genome Atlas (TCGA), Gene Expression Omnibus (GEO) dataset, Tumor Immune Estimation Resource (TIMER), Human Protein Atlas (HPA), and Gene Set Enrichment Analysis (GSEA) datasets.Results: In most tumors, BYSL expression is high and correlated with prognosis. Furthermore, BYSL gene alterations, bystin like protein phosphorylation, and bystin DNA methylation are linked to many types of tumorigenesis and immune invasion. Thirdly, translation and viral carcinogenesis may contribute to BYSL's tumor-pathogenic effects.Conclusions: Based on the results of the bioassay used in this study, BYSL may have potential as a biomarker to differentiate tumor tissue from normal tissue in most cancer patients, providing new research clues of how BYSL is regulated and thus the development of targeted therapies.
Assessment of heavy metals pollution in marine sediments from southwest of Mallorca island, Spain
Authors: Ardila Pedro Agustín Robledo , Alonso Rebeca Álvarez , Valsero Juan José Durán , García Raquel Morales , Cabrera Flor Árcega , Cosío Elizabeth Lamas , Laforet Sergio Raúl Durán
Published: June 2022

Anthropogenic activities in the urban, agrarian, or industrial areas are the main cause of heavy metals in sediments worldwide. Since the 1960s there have been submarine discharges through outfalls of poorly treated or untreated wastewater on the south coast of Mallorca island (Mediterranean Sea). In this study, the pollution of marine sediments is analyzed at a great number of points on the south of the seacoast. Heavy metals concentrations of As, Ba, Cd, Cr, Cu, Hg, Ni, Pb, Se, V, Zn, Total Inorganic Carbon (TIC), Sulfur, mineralogy, and grain size were analyzed. The objective is to evaluate the concentrations of toxic substances and their spatial distribution and the ranges that can negatively affect marine ecosystems and human health. In addition, the results obtained have been compared with standardized indices for marine sediments and a regional index has been developed with the background values of heavy metals obtained in the sediments of the study area. To obtain consistent conclusions, concentrations of heavy metals were classified with the Igeo Index. The concentrations of heavy metals obtained show as a great number of samples exceeded the limits considered for uncontaminated sediments according to the index applied. Elements such as Hg, Ba, Pb, and Cu showed high concentrations close to the outfalls and lower concentrations in zones far from these points. To support the assessment chemical processes, such as dissolution or chemical precipitation have been studied. The results also show as marine sediments can be a good trap for chemical elements and a good proxy to analyze the impact of anthropogenic activities in areas heavily pressured by humans, and the risk to the environment and human health.
Macroeconomy Driven By Climate Change
Authors: Toh Tze-Chuen
Published: June 2022

A simple econometric model predicting the effects of key drivers of climate change on the real GDP is constructed. It shows quantitatively how GDP is affected by carbon dioxide emissions and energy produced from renewable sources (excluding hydroelectricity). Moreover, the model also demonstrates that increasing energy consumption using fossil fuels at the expense of renewable sources negatively impacts GDP.JEL Classification: C51 , C52 , C53 , O44 , Q54
Research on Rose Classification Based on Neural Network Model
Authors: Hui Jiaojiao
Published: June 2022

As an important industry in China, rose has extremely important economic value and ornamental value. However, affected by human factors and planting environment, even roses of the same variety will have different levels of classification. In order to solve the low efficiency of wrong classification and missing classification caused by manual visual classification and realize the transformation of rose classification from manual judgment to machine independent recognition, this paper first performs data enhancement, image naming and other operations on 200 manually photographed yellow rose images of 5 levels of the same variety, and constructs an image database. Then, in Python language and tensorflow2.0 deep learning tool, design rose classification algorithm based on artificial neural network(ANN), rose classification algorithm based on convolution neural network(CNN) and rose classification algorithm based on feature extraction(FE) and artificial neural network(ANN). Finally, the experimental results show that the third algorithm significantly speeds up the running speed and significantly improves the average recognition rate compared with the first two algorithms, which effectively solves the problems of wrong or missing classification and low classification efficiency in manual intuitive classification, and realizes the transformation from manual classification to machine independent classification.
Adherence Therapy for adults with type 2 diabetes: a feasibility study of a randomised controlled trial
Authors: Alenazi Fatimah , Peddle Monica , Bressington Daniel , Mahzari Moeber , Gray Richard
Published: June 2022

BackgroundAdherence Therapy is a candidate intervention to promote consistent medication taking in people with type 2 diabetes. The aim of this study was to establish the feasibility of conducting a randomised controlled trial of Adherence Therapy in people with type 2 diabetes who were non-adherent with medication.MethodsAn open-label, single-centre, randomised controlled feasibility trial. Participants were randomly allocated to receive either eight sessions of telephone delivered Adherence Therapy or treatment as usual. Recruitment occurred during the COVID-19 pandemic. Outcome measures – adherence, beliefs about medication and HbA1c – were administered at baseline and after eight weeks or at the completion of treatment. Feasibility outcomes included the number of people initiated to participate in the trial that consented, completed study measures, finished treatment with adherence therapy, and dropped out of the trial.ResultsSeventy-eight people were screened of which 39 met eligibility criteria and were invited to take part in the trial. Thirteen people consented to participate, were enrolled in the trial and randomised. Five (71%) of the seven participants in the Adherence Therapy arm completed treatment. Baseline measures were completed by all participants. Week 8 (post-treatment) measures were completed by eight (62%) participants. Drop out may have been linked with a poor understanding of what was involved in taking part in the trial.ConclusionsIt may be feasible to conduct a full RCT of Adherence Therapy, but careful consideration should be given to developing effective recruitment strategies, consent procedures, rigorous field testing and clear support materials.Trial registrationThe trial was prospectively registered with the Australian New Zealand Clinical Trials Registry (ANZCTR), ACTRN12619000827134, on the 7th June 2019.
Identification and functional characterization of BICD2 as a candidate disease gene in an consanguineous family with dilated cardiomyopathy
Authors: Luo Kai , Zheng Chenqing , Luo Rong , Cao Xin , Sun Huajun , Ma Huihui , Huang Jichang , Yang Xu , Wu Xiushan , Li Xiaoping
Published: June 2022

BackgroundFamilial dilated cardiomyopathy (DCM) is a genetic cardiomyopathy that is associated with reduced left ventricle function or systolic function. Fifty-one DCM-causative genes have been reported, most of which are inherited in an autosomal dominant manner. However, recessive DCM-causative gene is rarely observed.MethodsWhole-exome sequencing (WES) was performed in a consanguineous family with DCM to identify candidate variants. Sanger sequencing was utilized to confirm the variant. We then checked the DCM candidate gene in 210 sporadic DCM cases. We next explored BICD2 function in both embryonic and adult bicd2-knockout zebrafish models. In vivo cardiac function of bicd2-knockout fish was detected by echocardiography and RNA-seq.ResultsWe identified an autosomal recessive and evolutionarily conserved missense variant, NM_001003800.1:c.2429G>A, in BICD2, which segregated with the disease phenotype in a consanguineous family with DCM. Furthermore, we confirmed the presence of BICD2 variants in 3 sporadic cases. Knockout of bicd2 resulted in partial embryonic lethality in homozygotes, suggesting a vital role for bicd2 in embryogenesis. Heart dilation and decreased ejection fraction, cardiac output and stroke volume were observed in bicd2-knockout zebrafish, suggesting a phenotype similar to human DCM. Furthermore, RNA-seq confirmed a larger transcriptome shift in in bicd2 homozygotes than in heterozygotes. Gene set enrichment analysis of bicd2-deficient fish showed the enrichment of altered gene expression in cardiac pathways and mitochondrial energy metabolism. Conclusionswe present the first finding of an autosomal recessive BICD2 variant that is associated with familial DCM, providing further insight into the molecular pathological mechanism of DCM.
Effects of dam building on Niche differentiation of Comammox Nitrospira in the main stream of the Three Gorges Reservoir area
Authors: Liu Shuang , Zhang Jiahui , Wang Yuchun , Hu Mingming , Bao Yufei , Li Shanze , Wen Jie , Zhao Jianwei
Published: June 2022

Comammox Nitrospira can completely oxidize ammonia nitrogen to nitrate, and the various habitats of comammox Nitrospira are an important guarantee for its survival. This study investigated comammox in river sediments in the Three Gorges Reservoir area. Comammox clade A and comammox clade B were detected in all samples, in which comammox clade A was dominant, and the OUTs detected from comammox clade B (14 OTUs) were more than those detected from comammox clade A. 1 (11 OTUs) and comammox clade A.2 (4 OTUs). The abundance of comammox Nitrospira clade A and clade B amoA genes in the main stream of the Three Gorges Reservoir indicated an increasing trend along the river stream, and reached the maximum in the of the reservoir area. The highest abundance of AOA amoA genes appeared in the upper stream section of the reservoir area. Comammox Nitrospira clade A exhibited the highest abundance (3.00×104±8782.37 copies/g), followed by comammox Nitrospira clade B (1.83×103±1019.82 copies/g), AOB (1.28×103±574.69 copies/g), AOA (1.73 ×102±48.05 copies/g). Both the abundances of comammox clade A and B were positively correlated with sediment pH, indicating that pH was an important environmental factor affecting the growth of comammox bacteria. The Mantel test chart indicated that overlying water dissolved oxygen (DO) in the reservoir area had a significant effect on the differentiation of comammox clade A into comammox clade A.1 and comammox clade A.2. This study confirms that the construction of the Three Gorges Dam has an impact on the niche differentiation of comammox Nitrospira in the main stream of the Three Gorges Reservoir area.
Risk factors associated with nerve injury following third molar surgery at an Australian university dental school: a 4-year retrospective study
Authors: Choudhari Shoaib , Park Joon Ha , Reher Vanessa , Reher Peter
Published: June 2022

Purpose: Nerve injury during the removal of third molars in oral surgery is a rare complication; however, it could lead to severe and long-term complications. For this study, data regarding third molar extractions were collected to identify predictive risk factors for inferior alveolar nerve and lingual nerve injuries. Methods: Clinical and radiographic data from Griffith University electronic records were obtained from January 2015 to December 2018. Out of 2826 extractions realised during that period, only fifteen nerve injuries were identified. Results: The incidence of nerve injury encountered was 0.35% for the inferior alveolar nerve and 0.18% related to the lingual nerve, both lower than incidences shown in previous studies. Moreover, no permanent nerve injuries were identified. The mean resolution time for the injuries was eight weeks. This study also identified several risk factors associated with inferior alveolar and lingual nerve injuries, such as the gender and age of the patients, the type of impaction and angulation of the tooth, the type of surgical technique and incision performed, and the proximity to the inferior alveolar canal. Conclusion: Overall, inferior alveolar nerve and lingual nerve injury incidences at Griffith University dental school were lower than the current literature, and no permanent nerve injury was encountered.
Generational Divide in Preferences for Forest Carbon Sequestration vs Preservation of Agricultural Landscapes
Authors: Liu Xiaozi , Lindhjem Henrik , Grimsrud Kristine , Leknes Einar , Tvinnereim Endre
Published: June 2022

Afforestation is considered an important measure for climate change mitigation. As land area that can be used for tree planting serves multiple purposes, it is vital to strike the right balance between climate and other objectives. We conducted a national survey in Norway to infer potential land use conflicts that may arise from executing a large-scale afforestation program. Citizens were presented three land use alternatives to replace formerly grazed agriculture land. We used manipulated landscape photos to illustrate policy impacts to the respondents and employed a combination of multiple correspondence analysis and a latent class regression model to reveal preference heterogeneity. Respondents cluster into three latent classes according to their value orientation, where approximately 20%, 40% and 40% citizens perceive forest carbon sequestration, agriculture, and recreation, respectively, as the most important land use functions to retain. Birth cohort is a strong predictor of the class membership: there is a stark generational difference between the baby boomers (born before 1960) and the generations born after 1980, with the baby boomers overwhelmingly supporting the continuation of agricultural landscapes. However, every ten-year reduction in respondent age increases the odds of a respondent belonging to the recreation- or forestation-preferring class (relative to the agriculture class) by factors of 1.9 and 1.4, respectively. Natural forests were perceived more favorably than mono-culture spruce plantation even among the respondents classified as the most climate concerned.
Sustaining the traditional ‘Kasuti embroidery’ by digital embroidery software
Authors: sarita Sarita Devi , Sushila Sushila , nisha Nisha Arya , sunil Sunil Changdev Talekar
Published: June 2022

Indian embroideries are popular all over the world and are highly liked (Sapra, K. 2015). Kasuti embroidery has its own regional identity and has crossed borders to be marketed in different parts of the world. Traditional Kasuti embroidery with ethnic designs and motifs with brilliant colours bear an individual hallmark of Indian textile design (Bennur and Gavai 2015). The success factor of Indian regional embroidery ‘Kasuti’ included: Customer specific designs and products, incorporating global styles” (Tiwari, A. and Anjali. 2019). In the present study, traditional ‘Kasuti’ embroidery motifs were explored and collected for the creative& innovative designing. Top-ranked motifs were selected for innovative design development and designs were developed with the help of Corel DRAW X3 software. After then digitize the selected designs using Wilcom E2 embroidery software. Respondents were evaluated the products on the basis of different parameters like techniques, aesthetic appeal, design placement, uniqueness of design and overall appearance. The study were found that all the developed products by using digital embroidery softwares are highly preferred by consumers, they are ready to purchase the articles and developed article were created the look of original hand embroidery.
Germline C1GALT1C1 Mutation Causes a Multisystemic Chaperonopathy with Global Deficiency of Core 1-derived O-Glycosylation
Authors: Erger Florian , Aryal Rajindra , Reusch Björn , Matsumoto Yasuyuki , Meyer Robert , Zeng Junwei , Knopp Cordula , Noel Maxence , Wenzel Andrea , Kohl Stefan , Tschernoster Nikolai , Rappl Gunter , Schröder-Braunstein Jutta , Seibert Felix , Thiele Holger , Häusler Martin , Weber Lutz , Büttner-Herold Maike , Elbracht Miriam , Cummings Sandra , Altmüller Janine , Habbig Sandra , Cummings Richard , Beck Bodo
Published: June 2022

The galactosyltransferase T-synthase exclusively synthesizes the T-antigen, a ubiquitous Oglycan core structure. Its function is dependent on its specific molecular chaperone, Cosmc (C1GALT1C1). In two maternal half-brothers with developmental delay, immunodeficiency, short stature, thrombocytopenia and acute kidney injury (AKI) resembling atypical hemolytic uremic syndrome, we identified the hemizygous variant c.59C > A (p.Ala20Asp) in Xchromosomal C1GALT1C1. Their heterozygous mother and maternal grandmother show an attenuated phenotype with skewed X-inactivation in blood. AKI in the male patients proved fully responsive to treatment with the complement inhibitor Eculizumab. This germline variant within the transmembrane domain of Cosmc causes dramatically reduced expression of the Cosmc chaperone with a significant reduction of Tsynthase enzyme activity, resulting in incomplete O-glycosylation. Transfection of patient cells with wildtype C1GALT1C1 partially rescued this glycosylation defect. We also detected very high levels of galactose-deficient IgA1 in all four affected individuals.
Identifying suitable source and optimum rate of silicon in rice at Ganges Delta Coastal Zone Soils
Authors: Haque Mohammad Asadul , Jahiruddin Md , Islam Md. , Hoque Md Fazlul , Hossain Md Baktear , Satter Md Abdus , Haque Md Enamul , Bell Richard William
Published: June 2022

Purpose: Silicon established as a beneficial nutrient element especially for cereals, however it’s suitable source and optimum rate of application as a chemical fertilizer still not recommended. The purpose of the study was to identify the suitable source and optimum rate of silicon for growing rice at Ganges delta coastal zone soils. Methodology: Two similar succeeding field experiments: one at early monsoon season with T. Aus rice and another at monsoon season with T. Aman rice in 2021 were carried out using a two factor split plot design having replicated thrice. The first factor was three different sources of silicon (silicic acid, sodium meta silicate and calcium silicate) and second factor was seven rates of silicon (0, 1, 2, 5, 10, 20 and 40 kg Si ha-1). Results: Using a quadratic model the optimum rate of silicic acid, sodium meta silicate and calcium silicate were 32.2, 25.7 and 23.6 kg Si ha-1 for T. Aus rice, and 42.9, 25.8 and 24.5 kg Si ha-1 for T. Aman rice, respectively. Calcium silicate was the best source of silicon as it shows best response with smaller rates of silicon. Sodium meta silicate had the second and silicic acid had the lowest performance. Increasing Si rates progressively increases the Si content, Si uptake and chlorophyll content of rice. Conclusion: Calcium silicate with 24 kg Si ha-1 is recommended for rice at Ganges delta coastal zone soils.
Multiplexed immunofluorescence analysis of CAF-markers, EZH2 and FOXM1 in Gastric Tissue: Associations with Clinicopathological Parameters and Clinical Outcomes
Authors: Sun Hui , Wang Xin , Wang Xu , Tan Cong , Weng Weiwei , Zhang Meng , Ni Shujuan , Wang Lei , Huang Dan , Sheng Weiqi , Xu Midie
Published: June 2022

Background: The aim of this study is to explore the expression and clinical relevance of CAF-associated markers, EZH2 and FOXM1 in gastric samples. Methods: Protein expression were detected and evaluated by multi-plex immunofluorescence (mIF) in 93 cases of gastric cancer (GC) and 31 cases of gastric intraepithelial neoplasia (GIN). The correlation among their expression, and the relationship of them with clinicopathological parameters and prognosis in GC were then analyzed.Results: FAP was specific expressed in the CAFs of GC samples, and thus be utilized as a CAF-associated marker in our subsequently analysis. The immunostaining of EZH2, FOXM1 and FAP were significantly upregulated in patients with GC tissues than in those normal gastric mucosa or GIN tissues. The average fluorescence intensity of FAP was slightly positive correlated with EZH2 in GC, GIN and normal samples, whereas the percent of FAP positive cells has no correlation with that of EZH2. Both the percent of positive cells and the average fluorescence intensity of FOXM1 were positive correlated with that of FAP and EZH2 in GC, GIN and normal samples, except for FOXM1 and EZH2 expression in normal tissue samples. No significant association was observed between FAP expression and any clinicopathological parameters, whereas the positive frequency of EZH2 and FOXM1 were correlated with tumor location significantly and tumor invasion depth, respectively. In addition, there was strong positive correlations between FAP protein expression and overall survival (OS) and disease-free survival (DFS), and EZH2 expression was positive associated with OS in patients with GC. Furthermore, EZH2 and FAP protein expression was an independent prognostic factor for OS and DFS, respectively. Conclusions: These results suggest that both EZH2 and FOXM1 expression was positive associated with CAFs abundance in GC. They may be potential cellular target for therapeutic intervention, especially in patients with abundant CAFs.
Immunization with a recombinant ASP-2/Transialidase chimeric protein induces robust protective immunity in both murine and canine models of Chagas’ disease
Authors: Gazzinelli Ricardo , de Castro Julia Teixeira , De Brito Rory Cristiane , Hojo-Souza Natalia , Azevedo Barbara , Salazar Natalia , Ferreira Camila , Junqueira Caroline , Fernandes Ana Paula , Vasconcelos Jose , Cardoso Jamille Mirelle , Aguiar-Soares Rodrigo , Vieira Paula , Carneiro Claudia , Salazar Andres , Caballero Otavia , Teixeira Santuza , Reis Alexandre
Published: June 2022

We have systematically shown that the Amastigote Surface Protein-2 (ASP-2) and Transialidase (TS) antigens either in the form of recombinant protein or encoded in the plasmids and human adenovirus 5 (hAd5) confer strong protection against different lineages of Trypanosoma cruzi parasites. Herein we generated a chimeric protein containing the most immunogenic regions for T and B cells from ASP-2 and TS (DTT-1) and evaluated its efficacy in comparison with our standard protocol of heterologous prime-boost using plasmids and hAd5. Our results show that mice immunized with DTT-1 protein together with Poly-ICLC (Hiltonol) become highly resistant to challenge with the Y strain of T. cruzi, showing a large decrease in tissue parasitism, parasitemia and no lethality. This protection lasted for at least 3 months after the last boost of immunization and an equivalent level of protection induced by the DNA/hAd5 protocol. DTT-1 induced high levels of T. cruzi-specific antibodies and IFNγ-producing T cells and protection was mediated by B lymphocytes, CD8 T cells and IFNγ. Finally, we evaluated the toxicity, immunogenicity and efficacy of the DTT-1 and DNA/hAd5 formulations in dogs. Mild collateral effects were detected at the site of vaccine inoculation. While the chimeric protein associated to Poly-ICLC induced high levels of antibodies and CD4+ T cell responses, the DNA/hAd5 induced no antibodies, but a strong CD8+ T cell response. Immunization with either vaccine formulations protected dogs against challenge with the Berenice strain of T. cruzi. Despite to the similar efficacy, we conclude that moving ahead with DTT-1 together with Hiltonol is advantageous over the hAd5 vaccine due to the cost-benefit for development and large-scale production.
Hyperinflation and its Association with Successful Transition to Home Ventilator Devices in Infants with Chronic Respiratory Failure and Severe Bronchopulmonary Dysplasia
Authors: Lagoski Megan , Reisfeld Michael , Carpenter Ryan , Lamorena Emilee , Goodman Denise , Murthy Karna
Published: June 2022

Objective
To estimate the association between lung hyperinflation and the time to successful transition to outpatient ventilators in infants with sBPD and chronic respiratory failure.
Design/Methods:
Infants with sBPD < 32 weeks’ gestation who received tracheostomies were identified. Hyperinflation was the main exposure. Time from tracheostomy to successful transition to the outpatient device was the main outcome. Kaplan-Meier and multivariable Cox proportional hazards were used to estimate the relationships between hyperinflation and the main outcome.
Results
Sixty-two infants were included; 26 (42%) were hyperinflated. Eleven died before transition, and 51 successfully transitioned. Hyperinflation was associated with both mortality (31% vs 8.3%, p = 0.02) and an increased duration (72 vs. 56 days) to successful transition (hazard ratio (HR) = 0.38, 95% CI: 0.19, 0.76, p = 0.006). Growth velocity was similar after tracheostomy placement.
Conclusions
In infants with chronic respiratory failure and sBPD < 32 weeks’ gestation, hyperinflation is related to mortality and inpatient morbidities.
Effect of Low Dose Denosumab on Bone Mineral Density in Postmenopausal Women with Osteoporosis After a Transition From 60mg Dose: A Prospective Observational Study
Authors: Khan Aliya A. , AbuAlrob Hajar , M’Hiri Iman , ALI Dalal S. , Dandurand Karel , Said Hosay , Alkassem Hisham , Hakami Yasser , Hweija Ismail , Iqbal Salman , Romanovschi Mihai Radu , Mehmood Shehryar , Zariffeh Heather , Guyatt Gordon , Ibrahim Quazi , Brignardello-Petersen Romina
Published: June 2022

Introduction:
Denosumab is an effective antiresorptive molecule and reduces the risk of fracture in postmenopausal osteoporosis. Cessation of denosumab therapy however is associated with rapid declines in bone mineral density (BMD), rises in bone remodelling and an increased risk of fracture. We evaluated the effect of low dose denosumab (30mg every 6 months) on the prevention of bone loss following a switch from standard dose (60mg of denosumab every 6 months) in a prospective observational study.
Methods
We recruited 114 women 50–90 years of age with postmenopausal osteoporosis at a moderate fracture risk without prior fragility fractures who had been on denosumab 60mg every 6 month. These women switched to low dose denosumab 30mg every 6 months. Mean % change in lumbar spine (LS), femoral neck (FN), total hip (TH) and 1/3 distal radius (1/3RAD) BMD at 12 and 24 months were evaluated. Predictors for change in BMD were explored. Subgroup analysis for patients on denosumab 60mg every 6 months for < 3 years and for ≥ 3 years before switching to low dose denosumab 30mg was explored.
Results
At 12 months, there was an increase in LS BMD mean % change (+ 2.03%, 95% CI 1.18–2.88, p < 0.001). BMD was stable at the hip and radial sites. Age was found to be a predictor of the mean % change of LS BMD for the overall sample. At 24 months, there was a further increase in LS BMD mean % change (+ 3.44%, 95% CI 1.74–5.12, p < 0.001), with stable BMD at other skeletal sites. The 12 month mean BMD % change at the LS (p = 0.015), FN (p < 0.001), TH (p < 0.001), and 1/3 RAD (p < 0.001) were found to be predictors of the 24 month mean BMD % change. No clinical fractures were reported during 24 months of follow up.
Conclusion
We observed stable BMD following a switch from denosumab 60mg every 6 months to 30mg every 6 monhts in this prospective observational study conducted in postmenopausal women at a moderate fracture risk
Functional Connectivity Uniqueness and Stability? A Signature of Cognitive and Psychiatric Problems in Children
Authors: Fu Zening , Liu Jingyu , Salman Mustafa , Sui Jing , Calhoun Vince
Published: June 2022

Brain functional connectivity (FC) derived from functional magnetic resonance imaging (fMRI) has been serving as a potential “fingerprint” for adults. However, intra-subject variation of FC can be substantial and carry biologically meaningful information, especially during adolescence, a time of intense brain changes. Here, for the first time, we performed a large-scale analysis on cross-scan FC stability and its association with a diverse range of health measures in children from the Adolescent Brain Cognitive Development (ABCD) data. Functional network connectivity (FNC), a network analog of FC, was extracted via an automated independent component analysis framework on 9071 subjects (age: 9 ~ 11 years) and compared across 4 scans. We found that the FNC profile can identify a given child from a large group with high accuracy (> 80%), though intra-subject variations exist cross-scan. The robustness of this finding is verified by replicating the child identification using the second-year scans and between longitudinal scans with a two-year interval. Moreover, cross-scan FNC stability was predictive of sleep condition, cognitive performance, and psychiatric problems in children, with higher stability correlated with better cognitive performance, longer sleep duration, and less psychotic expression. Meanwhile, parental psychopathology and prenatal exposure were associated with the FNC stability in their children. Overall, our findings show that a child’s connectivity profile is not only intrinsic but also exhibits reliable variability cross-scan, regardless of brain growth and development. The intra-subject connectivity stability may serve as a valuable biomarker to draw inferences on early cognitive and psychiatric behaviors in children.
Process and materials design for laser cladded inconel-625/tungsten carbide wear-resistant composite coatings
Authors: Olakanmi Eyitayo Olatunde , Hoosain Shaik , Lawal Sunday Albert , Pityana Sisa Lesley
Published: June 2022

Tailoring materials’ microstructural characteristics to meet mechanical functional requirements is quite critical in designing wear resistant coatings. To the best of our knowledge, no study had related carbide dissolution ratio (CDR) in laser cladded (LC) Inconel 625 coating and its microstructural parameters to its wear performance. Hence, this study explores how laser processing and materials parameters influence CDR, microhardness (MH) and volume of materials loss (𝑉𝑀𝐿) of fiber-laser deposited Inconel 625 composite coatings reinforced with tungsten carbide (WC-86) by employing response surface methodology (RSM) via central composite design (CCD). Furthermore, the nature of inter-relationship between the CDR in laser cladded Inconel 625 composite coatings, microstructural parameters (average mean free path and size of retained particles, and MH) as well as 𝑉𝑀𝐿 was explored to determine appropriate process and materials parameters to optimise the wear resistance of the coatings. A fully consolidated composite coating characterised with uniformly distributed retained WC-86 particle size of 40 𝜇m; mean free path of 30𝜇m within the Inconel 625 matrix; MH = 852 HV0.5; CDR = 77.08% has the most desirable wear resistance (VML = 9.42mm3) when processed with appropriate laser energy density (19.70 J/mm2), inconel content (70wt%) and shielding gas flow rates (6.00 l/min). This study provides new insight, for coating manufacturers, on how CDR and microstructural parameters can be manipulated as LC process and materials variables are altered with a view to designing most desirable wear resistant composite coating.
A Biomimetic Laminated Strategy Enabled Durable and Ultra-Sensitive MXene-based Flexible Electronics for Temperature Monitoring with Strain-Tolerant Performance
Authors: hao sanwei , Fu Qingjin , Meng Lei , Xu Feng , Yang Jun
Published: June 2022

The development of flexible thermistor epidermal electronics (FTEE) to satisfy high temperature resolution without strain induced signal distortion is of great significance but still challenging. Inspired by the nacre microstructure capable of restraining the stress concentration, we exemplified a versatile MXene-based thermistor elastomer sensor (TES) platform that significantly alleviated the strain interference by the biomimetic laminated strategy combining with the in-plane stress dissipation and nacre-mimetic hierarchical architecture, delivering competitive advantages of superior thermosensitivity (-1.32% °C− 1), outstanding temperature resolution (~ 0.3°C), and unparalleled mechanical durability (20000 folding fatigue cycles), together with considerable improvement in strain-tolerant thermosensation over commercial thermocouple in exercise scenario. By a combination of theoretical model simulation, microstructure observation, and superposed signal detection, the authors further revealed the underlying temperature and strain signal decoupling mechanism that substantiated the generality and customizability of the nacre-mimetic strategy, possessing insightful significance of fabricating FTEE for static and dynamic temperature detection.
Study on the learning curve during the transition from direct lateral to direct anterior approach in total hip arthroplasty : a consecutive one hundred and twenty-seven case series
Authors: Chu Miao , Zhou Jiangang , Wang Yong , Jiang Tao , Bao Weiguo
Published: June 2022

Background: Direct anterior approach (DAA) for primary hip arthroplasty has received increasing attention recently. However, there have been no reports focused on the learning curve when transitioning from direct lateral approach(DLA) to DAA. This study evaluates a single surgeon’s learning curve by examining Procedure Time (PT), Operation Preparation Time (OPT), Operating Room Time(ORT) and other data. Methods: From 2019 to 2021, a single surgeon’s first 100 cases with DAA and 27 cases with DLA between the learning cure were reviewed. PT, OPT, ORT and other data were analyzed. Results: Compared with DLA, PT, OPT and ORT had a sharp rise in the first 10 cases. There was no statistical significance in PT after 20 cases, ORT after 30 cases, and OPT after 50 cases. The difference of PT and ORT between group 1(DLA) and group 2(DAA, 1-51 cases), group 2 and group 3(DAA, 51-100 cases) were statistically significant. OPT of Group 1 was the shortest, increased significantly in group 2, gradually decreased in group 3. There were significant differences in blood loss between group 1 and group 3, between group 2 and group 3. 4 complications in total, 1 case in group 1, 3 cases in group 2. There were no significant differences in blood transfusion, length of stay and complication rate. Conclusions: When transitioning from DLA to DAA, an experienced surgeon may be low efficient, with longer PT, OPT and ORT. Great changes appeared after DAA 50 cases, complications and blood loss could be controlled within the safe range.