bluuebunny/crossref_metadata_2025 huggingface dataset:
Cholinesterase Inhibitors from an Endophytic Fungus Aspergillus niveus Fv-er401: Metabolomics, Isolation and Molecular Docking
Authors: Hamed Ahmed A. , El-Shiekh Riham A. , Mohamed Osama G. , Aboutabl Elsayed A. , Fathy Fify I. , Fawzy Ghada A. , Al-Taweel Areej M. , Elsayed Tarek R. , Tripathi Ashootosh , Al-Karmalawy Ahmed A.
Published: March 2023

Alzheimer’s disease poses a global health concern with unmet demand requiring creative approaches to discover new medications. In this study, we investigated the chemical composition and the anticholinesterase activity of Aspergillus niveus Fv-er401 isolated from Foeniculum vulgare (Apiaceae) roots. Fifty-eight metabolites were identified using UHPLC-MS/MS analysis of the crude extract. The fungal extract showed acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) inhibitory effects with IC50 53.44 ± 1.57 and 48.46 ± 0.41 µg/mL, respectively. Two known metabolites were isolated, terrequinone A and citrinin, showing moderate AChE and BuChE inhibitory activity using the Ellman’s method (IC50 = 11.10 ± 0.38 µg/mL and 5.06 ± 0.15 µg/mL, respectively for AChE, and IC50 15.63 ± 1.27 µg/mL and 8.02 ± 0.08 µg/mL, respectively for BuChE). As evidenced by molecular docking, the isolated compounds and other structurally related metabolites identified by molecular networking had the required structural features for AChE and BuChE inhibition. Where varioxiranol G (−9.76 and −10.36 kcal/mol), penicitrinol B (−9.50 and −8.02 kcal/mol), dicitrinol A (−8.53 and −7.98 kcal/mol) and asterriquinone CT5 (−8.02 and −8.25 kcal/mol) showed better binding scores as AChE and BuChE inhibitors than the co-crystallized inhibitor (between −7.89 and 7.82 kcal/mol) making them promising candidates for the development of new drugs to treat Alzheimer’s.
Combined Description of the Equation of State and Diffusion Coefficient of Liquid Water Using a Two-State Sanchez–Lacombe Approach
Authors: Ginzburg Valeriy V. , Fazio Enza , Corsaro Carmelo
Published: March 2023

Water is one of the most important compounds on Earth, yet its material properties are still poorly understood. Here, we use a recently developed two-state, two-(time)scale (TS2) dynamic mean-field model combined with the two-state Sanchez–Lacombe (SL) thermodynamic theory in order to describe the equation of state (density as a function of temperature and pressure) and diffusivity of liquid water. In particular, it is shown that in a relatively wide temperature and pressure range (160 K < T < 360 K; 0 < P < 100 MPa), density and self-diffusion obey a special type of dynamic scaling, similar to the “τTV” scaling of Casalini and Roland, but with the negative exponent γ. The model predictions are consistent with experimental data. The new equation of state can be used for various process models and generalized to include multicomponent mixtures.
Insights into the Structures of Bilirubin and Biliverdin from Vibrational and Electronic Circular Dichroism: History and Perspectives
Authors: Longhi Giovanna , Ghidinelli Simone , Abbate Sergio , Mazzeo Giuseppe , Fusè Marco , Boiadjiev Stefan E. , Lightner David A.
Published: March 2023

In this work we review research activities on a few of the most relevant structural aspects of bilirubin (BR) and biliverdin (BV). Special attention is paid to the exocyclic C=C bonds being in mostly Z rather than E configurations, and to the overall conformation being essentially different for BR and BV due to the presence or absence of the double C=C bond at C-10. In both cases, racemic mixtures of each compound of either M or P configuration are present in achiral solutions; however, imbalance between the two configurations may be easily achieved. In particular, results based on chiroptical spectroscopies, both electronic and vibrational circular dichroism (ECD and VCD) methods, are presented for chirally derivatized BR and BV molecules. Finally, we review deracemization experiments monitored with ECD data from our lab for BR in the presence of serum albumin and anesthetic compounds.
Synthetic Strategies of Supported Pd-Based Bimetallic Catalysts for Selective Semi-Hydrogenation of Acetylene: A Review and Perspectives
Authors: Cao Xinxiang , Jang Ben W.-L. , Hu Jiaxue , Wang Lei , Zhang Siqi
Published: March 2023

Selective semi-hydrogenation of acetylene is an extremely important reaction from both industrial and theoretical perspectives. Palladium, due to its unique chemical and physical properties, is the most active and currently irreplaceable metal for this reaction in industry, but the poor catalytic selectivity towards ethylene is also its inherent shortcoming. Introducing a secondary metal to tune a geometric and electronic structures of Pd nanoparticles and to create a synergistic effect is the most widely used strategy to effectively improve the overall catalytic performance of Pd-based catalysts. Thus, various supported Pd-based bimetallic catalysts for selective semi-hydrogenation of acetylene have been exploited in the past decade. Timely comparison, analysis, and summarizing of various preparation methods may offer a beneficial reference for the subsequent development of such catalysts. In this context, herein, the advances in synthesis strategies of catalysts, including nano-catalysts, single atom alloys (SAAs), as well as bimetallic dual atom catalysts are summarized systematically. Their advantages and disadvantages are comparatively discussed. Finally, future perspectives for the synthetic strategies of supported Pd-based bimetallic catalysts for selective semi-hydrogenation of acetylene are proposed.
A Structural-Reporter Group to Determine the Core Conformation of Sialyl Lewisx Mimetics
Authors: Wagner Beatrice , Binder Florian P. C. , Jiang Xiaohua , Mühlethaler Tobias , Preston Roland C. , Rabbani Said , Smieško Martin , Schwardt Oliver , Ernst Beat
Published: March 2023

The d-GlcNAc moiety in sialyl Lewisx (sLex, 1) acts predominantly as a linker to position the d-Gal and the l-Fuc moieties in the bioactive spatial orientation. The hypothesis has been made that the NHAc group of GlcNAc pushes the fucose underneath the galactose and, thus, contributes to the stabilization of the bioactive conformation of the core of sLex (1). To test this hypothesis, GlcNAc mimetics consisting of (R,R)-1,2-cyclohexanediols substituted with alkyl and aryl substituents adjacent to the linking position of the fucose moiety were synthesized. To explore a broad range of extended and spatially demanding R-groups, an enzymatic approach for the synthesis of 3-alkyl/aryl-1,2-cyclohexanediols (3b-n) was applied. These cyclohexanediol derivatives were incorporated into the sLex mimetics 2b-n. For analyzing the relationship of affinity and core conformation, a 1H NMR structural-reporter-group concept was applied. Thus, the chemical shift of H-C5Fuc proved to be a sensitive indicator for the degree of pre-organization of the core of this class of sLex mimetics and therefore could be used to quantify the contribution of the R-groups.
Structure-Based Profiling of Potential Phytomolecules with AKT1 a Key Cancer Drug Target
Authors: Mirza Zeenat , Karim Sajjad
Published: March 2023

Identifying cancer biomarkers is imperative, as upregulated genes offer a better microenvironment for the tumor; hence, targeted inhibition is preferred. The theme of our study is to predict molecular interactions between cancer biomarker proteins and selected natural compounds. We identified an overexpressed potential molecular target (AKT1) and computationally evaluated its inhibition by four dietary ligands (isoliquiritigenin, shogaol, tehranolide, and theophylline). The three-dimensional structures of protein and phytochemicals were retrieved from the RCSB PDB database (4EKL) and NCBI’s PubChem, respectively. Rational structure-based docking studies were performed using AutoDock. Results were analyzed based primarily on the estimated free binding energy (kcal/mol), hydrogen bonds, and inhibition constant, Ki, to identify the most effective anti-cancer phytomolecule. Toxicity and drug-likeliness prediction were performed using OSIRIS and SwissADME. Amongst the four phytocompounds, tehranolide has better potential to suppress the expression of AKT1 and could be used for anti-cancer drug development, as inhibition of AKT1 is directly associated with the inhibition of growth, progression, and metastasis of the tumor. Docking analyses reveal that tehranolide has the most efficiency in inhibiting AKT1 and has the potential to be used for the therapeutic management of cancer. Natural compounds targeting cancer biomarkers offer less rejection, minimal toxicity, and fewer side effects.
Patient-Reported Quality of Care for Osteoarthritis in General Practice in South Tyrol, Italy: Protocol for Translation, Validation and Assessment of the OsteoArthritis Quality Indicator Questionnaire (OA-QI)
Authors: Wiedermann Christian J. , Marino Pasqualina , van der Zee-Neuen Antje , Mastrobuono Isabella , Mahlknecht Angelika , Barbieri Verena , Wildburger Sonja , Fuchs Julia , Capici Alessandra , Piccoliori Giuliano , Engl Adolf , Østerås Nina , Ritter Markus
Published: March 2023

Background: Evidence-based recommendations for the treatment of knee and hip osteoarthritis are similar internationally. Nevertheless, clinical practice varies across countries. Instruments for measuring quality have been developed to improve health care through targeted interventions. Studies on health service quality must consider the structural and cultural characteristics of countries, because each of their strengths and weaknesses differ. However, such instruments for health-related patient-reported outcomes for osteoarthritis have not yet been validated in German and Italian languages. Objectives: In order to be able to set targeted measures for the improvement of prevention and non-surgical treatment of osteoarthritis in South Tyrol, Italy, the quality of care must be recorded. Therefore, the aim of the project is to update, translate, and validate the OsteoArthritis Quality Indicator (OA-QI) questionnaire version 2, an established and validated questionnaire in Norwegian and English, for Germany and Italy. The second aim is to determine the quality of care for osteoarthritis of the hip and knee in a sample of patients who consult general practice in South Tyrol, and for comparison with patients who are admitted to rehabilitative spa-treatments for osteoarthritis in the state of Salzburg, Austria. Discussion: The results of this study will enable the identification and closure of gaps in osteoarthritis care. Although it is expected that body weight and exercise will play special roles, other areas of nonsurgical care might also be involved.
Interventions to Increase Physical Activity in Community-Dwelling Older Adults in Regional and Rural Areas: A Realist Synthesis Review Protocol
Authors: Cousins Stephen , McKechnie Rebecca , Jackman Patricia , Middleton Geoff , Rasekaba Tshepo , Blackberry Irene
Published: March 2023

The importance of physical activity (PA) for the health and wellbeing of older adults is well documented, yet many older adults are insufficiently active. This issue is more salient in regional and rural areas, where evidence of the most critical components of interventions that explain PA participation and maintenance in older populations is sparse. This realist review will (1) systematically identify and synthesise literature on PA interventions in community-dwelling older adults in regional and rural areas, and (2) explore how and why those interventions increase PA in that population. Using a realist synthesis framework and the behaviour change wheel (BCW), context–mechanism–outcome (C-M-O) patterns of PA interventions for older adults in regional and rural areas will be synthesised. Thematic analysis will be employed to compare, contrast, and refine emerging C-M-O patterns to understand how contextual factors trigger mechanisms that influence regional and rural community-dwelling older adults’ participation in PA interventions. This realist review will be the first to adopt a BCW analysis and a realist synthesis framework to explore PA interventions in community-dwelling older adults in regional and rural areas. This review will provide recommendations for evidence-based interventions to improve PA participation and adherence by revealing the important mechanisms apparent in this context. Systematic review registration: (PROSPERO CRD42023402499).
Location- and Physical-Activity-Based Application for Japanese Vocabulary Acquisition for Non-Japanese Speakers
Authors: Tran Nguyen , Kajimura Shogo , Shibuya Yu
Published: March 2023

There are various mobile applications to support foreign-language learning. While providing interactive designs and playful games to keep learners interested, these applications do not focus on motivating learners to continue learning after a long time. Our goal for this study was to develop an application that guides learners to achieve small goals by creating small lessons that are related to their real-life situations, with a main focus on vocabulary acquisition. Therefore, we present MiniHongo, a smartphone application that recognizes learners’ current locations and activities to compose lessons that comprise words that are strongly related to the learners’ real-time situations and can be studied in a short time period, thereby improving user motivation. MiniHongo uses a cloud service for its database and public application programming interfaces for location tracking. A between-subject experiment was conducted to evaluate MiniHongo, which involved comparing it to two other versions of itself. One composed lessons without location recognition, and the other composed lessons without location and activity recognition. The experimental results indicate that users have a strong interest in learning Japanese with MiniHongo, and some difference was found in how well users could memorize what they learned via the application. It is also suggested that the application requires improvements.
Strain Measurement in Single Crystals by 4D-ED
Authors: Lábár János L. , Pécz Béla , van Waveren Aiken , Hallais Géraldine , Desvignes Léonard , Chiodi Francesca
Published: March 2023

A new method is presented to measure strain over a large area of a single crystal. The 4D-ED data are collected by recording a 2D diffraction pattern at each position in the 2D area of the TEM lamella scanned by the electron beam of STEM. Data processing is completed with a new computer program (available free of charge) that runs under the Windows operating system. Previously published similar methods are either commercial or need special hardware (electron holography) or are based on HRTEM, which involves limitations with respect to the size of the field of view. All these limitations are overcome by our approach. The presence of defects results in small local changes in orientation that change the subset of experimentally available diffraction spots in the individual patterns. Our method is based on a new principle, namely fitting a lattice to (a subset of) measured diffraction spots to improve the precision of the measurement. Although a spot to be measured may be missing in some of the patterns even the missing spot can be precisely measured by the lattice determined from the available spots. Application is exemplified by heavily boron-doped silicon with intended usage as a low-temperature superconductor in qubits.
Direct CVD Growth of Transferable 3D Graphene for Sensitive and Flexible SERS Sensor
Authors: Kim Su Han , Jiang Shiru , Lee Sang-Shin
Published: March 2023

Three-dimensional (3D) graphene (Gr) has been successfully grown on a patterned sapphire substrate (PSS) with very low mismatch between Gr and the sapphire nanostructure through metal-catalyst-assisted chemical vapor deposition (CVD). However, the transfer of the 3D Gr film without compromising the structural integrity of Gr is challenging because of the low etching rate of PSS. For easy and high-quality transfer of 3D Gr, we propose to coat a transfer-support layer (TSL) on PSS before direct CVD growth of 3D Gr. The TSL is directly deposited on PSS by atomic layer deposition without causing any structural changes in the substrate, as verified through atomic force microscopy (AFM). Few-layer 3D Gr is conformally produced along the surface of the TSL/PSS and successfully transferred onto a flexible substrate through wet-etching transfer, as confirmed by scanning electron microscopy, AFM, and Raman spectroscopy studies. We also present the fabrication of a sensitive and flexible surface-enhanced Raman scattering sensor based on 3D Gr on PMMA with high detection performance for low concentrations of R6G (10−9 M). The proposed transfer method with TSL is expected to broaden the use of 3D graphene in next-generation device applications.
Post-Processing Trimming of Silicon Photonic Devices Using Femtosecond Laser
Authors: Wu Yating , Shang Hongpeng , Zheng Xiaorui , Chu Tao
Published: March 2023

Fabrication errors inevitably occur in device manufacturing owing to the limited processing accuracy of commercial silicon photonic processes. For silicon photonic devices, which are mostly processing-sensitive, their performances usually deteriorate significantly. This remains an unsolved issue for mass production, particularly for passive devices, because they cannot be adjusted once fixed in processes. This study presents a post-processing trimming method to compensate for fabrication errors by changing the cladding equivalent refractive indices of devices with femtosecond lasers. The experimental results show that the resonant wavelengths of micro-ring resonators can be regularly shifted within their free spectral range via tuning the illuminating area, focusing position, emitting power, and scanning speed of the trimming femtosecond laser with an acceptable loss increase. These experiments, as well as the trimming experiments in improving the phase balance of Mach-Zehnder interferometer switches, indicate that the femtosecond laser trimming method is an effective and fast method for silicon photonic devices.
Managing Native Hip Protrusio: Simplified Classification and Surgical Recommendations
Authors: Ani Lidia , Radford Zachary , Rubin Lee E.
Published: March 2023

Protrusio acetabuli is a unique osteological condition that has been long described in the literature and is known to potentially increase both the surgical complexity and the risk of complications when performing total hip arthroplasty. Although grading systems for native hip protrusio have been described in the past, there has not yet been a widely adopted classification system that categorizes the condition into separate classes in order to guide management. We propose a novel classification system with the goal of simplifying and standardizing the management of protrusio acetabuli in the context of modern total hip arthroplasty. This classification system describes protrusio based on the relationship of the femoral head to the ilioischial and iliopectineal lines, allowing for a more reproducible and consistent categorization of pathology. We also discuss general recommendations and technical pearls for total hip arthroplasty in the protrusio patient population, including the use of strategic soft tissue releases, fluoroscopy, navigation, bone graft, and augments.
Role of Innate Interferon Responses at the Ocular Surface in Herpes Simplex Virus-1-Induced Herpetic Stromal Keratitis
Authors: Ren Jiayi , Antony Ferrin , Rouse Barry T. , Suryawanshi Amol
Published: March 2023

Herpes simplex virus type 1 (HSV-1) is a highly successful pathogen that primarily infects epithelial cells of the orofacial mucosa. After initial lytic replication, HSV-1 enters sensory neurons and undergoes lifelong latency in the trigeminal ganglion (TG). Reactivation from latency occurs throughout the host’s life and is more common in people with a compromised immune system. HSV-1 causes various diseases depending on the site of lytic HSV-1 replication. These include herpes labialis, herpetic stromal keratitis (HSK), meningitis, and herpes simplex encephalitis (HSE). HSK is an immunopathological condition and is usually the consequence of HSV-1 reactivation, anterograde transport to the corneal surface, lytic replication in the epithelial cells, and activation of the host’s innate and adaptive immune responses in the cornea. HSV-1 is recognized by cell surface, endosomal, and cytoplasmic pattern recognition receptors (PRRs) and activates innate immune responses that include interferons (IFNs), chemokine and cytokine production, as well as the recruitment of inflammatory cells to the site of replication. In the cornea, HSV-1 replication promotes type I (IFN-α/β) and type III (IFN-λ) IFN production. This review summarizes our current understanding of HSV-1 recognition by PRRs and innate IFN-mediated antiviral immunity during HSV-1 infection of the cornea. We also discuss the immunopathogenesis of HSK, current HSK therapeutics and challenges, proposed experimental approaches, and benefits of promoting local IFN-λ responses.
Differential Analysis of Key Proteins Related to Fibrosis and Inflammation in Soluble Egg Antigen of Schistosoma mansoni at Different Infection Times
Authors: Chen Ying-Chou , Chen I-An , Peng Shih-Yi , Cheng Po-Ching
Published: March 2023

Schistosomiasis is a major global health problem. Schistosomes secrete antigens into the host tissue that bind to chemokines or inhibit immune cell receptors, regulating the immune responses to allow schistosome development. However, the detailed mechanism of chronic schistosome infection-induced liver fibrosis, including the relationship between secreted soluble egg antigen (SEA) and hepatic stellate cell (HSC) activation, is still unknown. We used mass spectrometry to identify the SEA protein sequences from different infection weeks. In the 10th and 12th infection weeks, we focused on the SEA components and screened out the special protein components, particularly fibrosis- and inflammation-related protein sequences. Our results have identified heat shock proteins, phosphorylation-associated enzymes, or kinases, such as Sm16, GSTA3, GPCRs, EF1-α, MMP7, and other proteins linked to schistosome-induced liver fibrosis. After sorting, we found many special proteins related to fibrosis and inflammation, but studies proving their association with schistosomiasis infection are limited. Follow-up studies on MICOS, MATE1, 14-3-3 epsilon, and CDCP1 are needed. We treated the LX-2 cells with the SEA from the 8th, 10th, and 12th infection weeks to test HSC activation. In a trans-well cell model in which PBMCs and HSCs were co-cultured, the SEA could significantly induce TGF-β secretion, especially from the 12th week of infection. Our data also showed that TGF-β secreted by PBMC after the SEA treatment activates LX-2 and upregulates hepatic fibrotic markers α-SMA and collagen 1. Based on these results, the CUB domain-containing protein 1 (CDCP1) screened at the 12th infection week could be investigated further. This study clarifies the trend of immune mechanism variation in the different stages of schistosome infection. However, how egg-induced immune response transformation causes liver tissue fibrosis needs to be studied further.
Cellular Immune Profiling of Lung and Blood Compartments in Patients with SARS-CoV-2 Infection
Authors: Santinelli Letizia , Lazzaro Alessandro , Sciarra Francesca , Maddaloni Luca , Frasca Federica , Fracella Matteo , Moretti Sonia , Borsetti Alessandra , Bugani Ginevra , Alessandri Francesco , Zullino Veronica , Ruberto Franco , Pugliese Francesco , Sorrentino Leonardo , Gianfrilli Daniele , Isidori Andrea , Venneri Mary Anna , Mastroianni Claudio M. , Ceccarelli Giancarlo , d’Ettorre Gabriella
Published: March 2023

Background: SARS-CoV-2 related immunopathology may be the driving cause underlying severe COVID-19. Through an immunophenotyping analysis on paired bronchoalveolar lavage fluid (BALF) and blood samples collected from mechanically ventilated patients with COVID-19-associated Acute Respiratory Distress Syndrome (CARDS), this study aimed to evaluate the cellular immune responses in survivors and non-survivors of COVID-19. Methods: A total of 36 paired clinical samples of bronchoalveolar lavage fluid (BALF) mononuclear cells (BALF-MC) and peripheral blood mononuclear cells (PBMC) were collected from 18 SARS-CoV-2-infected subjects admitted to the intensive care unit (ICU) of the Policlinico Umberto I, Sapienza University Hospital in Rome (Italy) for severe interstitial pneumonia. The frequencies of monocytes (total, classical, intermediate and non-classical) and Natural Killer (NK) cell subsets (total, CD56bright and CD56dim), as well as CD4+ and CD8+ T cell subsets [naïve, central memory (TCM) and effector memory (TEM)], and those expressing CD38 and/or HLADR were evaluated by multiparametric flow cytometry. Results: Survivors with CARDS exhibited higher frequencies of classical monocytes in blood compared to non-survivors (p < 0.05), while no differences in the frequencies of the other monocytes, NK cell and T cell subsets were recorded between these two groups of patients (p > 0.05). The only exception was for peripheral naïve CD4+ T cells levels that were reduced in non-survivors (p = 0.04). An increase in the levels of CD56bright (p = 0.012) and a decrease in CD56dim (p = 0.002) NK cell frequencies was also observed in BALF-MC samples compared to PBMC in deceased COVID-19 patients. Total CD4+ and CD8+ T cell levels in the lung compartment were lower compared to blood (p = 0.002 and p < 0.01, respectively) among non-survivors. Moreover, CD38 and HLA-DR were differentially expressed by CD4+ and CD8+ T cell subsets in BALF-MC and in PBMC among SARS-CoV-2-infected patients who died from COVID-19 (p < 0.05). Conclusions: These results show that the immune cellular profile in blood and pulmonary compartments was similar in survivors and non-survivors of COVID-19. T lymphocyte levels were reduced, but resulted highly immune-activated in the lung compartment of patients who faced a fatal outcome.
Recent Advances in Optical Injection Locking for Visible Light Communication Applications
Authors: Liu Xingchen , Hu Junhui , Bian Qijun , Yi Shulan , Ma Yingnan , Shi Jianyang , Li Ziwei , Zhang Junwen , Chi Nan , Shen Chao
Published: March 2023

The introduction of visible light communication (VLC) technology could increase the capacity of existing wireless communication systems towards 6G networks. In practice, VLC can make good use of lighting system infrastructures to transmit data using light fidelity (Li-Fi). The use of semiconductor light sources, including light-emitting diodes (LEDs) and laser diodes (LDs) are essential to VLC technology because these devices are energy-efficient and have long lifespans. To achieve high-speed VLC links, various technologies have been utilized, including injection locking. Optical injection locking (OIL) is an optical frequency and phase synchronization technique that has been implemented in semiconductor laser systems for performance enhancement. High-performance optoelectronic devices with narrow linewidth, wide tunable emission, large modulation bandwidth and high data transmission rates are desired for advanced VLC. Thus, the features of OIL could be promising for building high-performance VLC systems. In this paper, we present a comprehensive review of the implementation of the injection-locking technique in optical communication systems. The enhancement of characteristics through OIL is elucidated. The applications of OIL in VLC systems are discussed. The prospects of OIL for future VLC systems are evaluated.
Dispersion-Oriented Inverse Design of Photonic-Crystal Fiber for Four-Wave Mixing Application
Authors: Gan Linqiao , Yu Fei , Wang Yazhou , Wang Ning , Zhu Xinyue , Hu Lili , Yu Chunlei
Published: March 2023

In this paper, we demonstrate the application of a deep learning neural network (DNN) in the dispersion-oriented inverse design of photonic-crystal fiber (PCF) for the fine-tuning of four-wave mixing (FWM). The empirical formula of PCF dispersion is applied instead of numerical simulation to generate a large dataset of phase-matching curves of various PCF designs, which significantly improves the accuracy of the DNN prediction. The accuracies of DNNs’ predicted PCF structure parameters are all above 95%. The simulations of the DNN-predicted PCFs structure demonstrate that the FWM wavelength has an average numerical mean square error (MAE) of 1.92 nm from the design target. With the help of DNN, we designed and fabricated a specific PCF for wavelength conversion via FWM from 1064 nm to 770 nm for biomedical imaging applications. Pumped by a microchip laser at 1064 nm, the signal wavelength is measured at 770.2 nm.
Simulated Studies of Polarization-Selectivity Multi-Band Perfect Absorber Based on Elliptical Metamaterial with Filtering and Sensing Effect
Authors: Wang Yinghua , Kong Yubo , Xu Shitong , Li Jie , Liu Guangqiang
Published: March 2023

In this work, the Ag-SiO2-Ag metamaterial with elliptical nano-slits was proposed to investigate the multi-band polarization-dependent perfect absorber. It was found that multi-band perfect absorptions can be induced under TE and TM-polarized illuminations. Simulation results showed that the absorption peaks for TE-polarized wave appeared at 337.6 THz and 360.0 THz with 98.5% and 97.6% absorbance, respectively. Conversely, the absorption peaks for TM-polarized wave appeared at 325.7 THz and 366.1 THz with 96.3% and 97.9% absorbance, respectively. As a result, the elliptical metamaterial presented polarization-selectivity property for perfect absorption, and so, the metamaterial can filter out different frequencies of TE- and TM-reflected waves, i.e., the elliptical metamaterial can be used as a reflecting filter. In addition, this work studied the sensing performance of the elliptical metamaterial and showed that the dual-band sensing performances were different at low and high frequencies. The sensitivities (S) to the refractive index reached up to 151.1 THz/RIU and 120.8 THz/RIU for the TE and TM-polarized waves around 337.6 THz and 325.7 THz, which provide promising potential in near-infrared photoelectric sensor and detector. However, both the absorption frequency and intensity of TM-polarized wave were insensitive to the refractive index of the medium around 366.1 THz, and so, the study provides a theoretical basis for infrared stealth of different media.
Frequency Comb Generation Based on Brillouin Random Lasing Oscillation and Four-Wave Mixing Assisted with Nonlinear Optical Loop Mirror
Authors: Pang Yuxi , Ma Shaonian , Ji Qiang , Zhao Xian , Li Yongfu , Qin Zengguang , Liu Zhaojun , Xu Yanping
Published: March 2023

A frequency comb generator (FCG) based on dual-cavity Brillouin random fiber lasing oscillation in the 1.5 μm telecon spectral window is established and experimentally demonstrated. In the half-open main cavity of the dual cavity, the stimulated Brillouin scattering in highly nonlinear fiber (HNLF) and Rayleigh scattering in single-mode fiber are employed to provide sufficient Brillouin gain and the randomly distributed feedback, respectively, for random mode resonance. The sub-cavity includes an Er-doped fiber amplifier to couple back and boost lower-order Stokes and anti-Stokes light for the cascade of stimulated Brillouin scattering to generate multiple higher-order Stokes and anti-Stokes light. Meanwhile, efficient four-wave mixing is stimulated in the HNLF-based main cavity, further enhancing the number and intensity of the resonant Stokes and anti-Stokes light. By taking advantages of the unique transmission characteristics of nonlinear optical loop mirrors, the power deviation between Stokes and anti-Stokes lines is further optimized with 17 orders of stable Stokes lines and 15 orders of stable anti-Stokes lines achieved within the 10 dB power deviation, with maximum optical signal-to-noise ratio (OSNR) of ~22 dB and ~17 dB and minimum OSNR of ~10 dB and ~7.5 dB for Stokes and anti-Stokes lines, respectively. In addition, the dynamic characteristics of the proposed FCG have been experimentally investigated. Such an FCG with fixed frequency spacing will find promising applications in fields of optical communication, microwave, optical sensing, etc.
High-Performance Dual-Channel Photonic Crystal Terahertz Wave Modulator Based on the Defect Mode Disappearance of a Combined Microcavity
Authors: Li Gaofang , Tan Jie , Xu Yanxia , Cui Haoyang , Tang Bo , Jiao Zhejing , Zhou Wei , Zeng Jundong , Xia Nenghong
Published: March 2023

With the working frequency of wireless communication systems moving to a higher terahertz (THz) band, the design of high-performance THz wave modulators has become a pivotal issue to be tackled urgently in THz communication. In this paper, we design a high-performance dual-channel photonic crystal modulator to enable ON–OFF regulation of the THz wave based on the defect mode disappearance of combined microcavities. The modulator introduces Y-type line defects into silicon rod photonic crystals as a dual-channel waveguide and the point defects and ring resonator form a combined microcavity. Due to the refractive index of the ring resonator filler, gallium arsenide, it is tunable with pump light excitation, and the defect mode frequency of the combined microcavity can be dynamically changed. Under pump excitation with a wavelength of 810 nm and an intensity of 0.4 μJ/cm2, 1.34 THz and 1.83 THz dual-channel waves can be OFF due to the defect mode disappearance of the combined microcavity. This is simulated by the time-domain steady-state response and steady-state THz wave field intensity distribution of the modulator by the finite-difference time-domain method. The results indicate that the dual-channel modulator has large modulation depths of 100% and 99.7%, high modulation rates of 4.05 GHz and 4.17 GHz, and low insertion losses of 0.31 dB and 0.18 dB, which lays foundation for the development of high-speed and low-loss THz communication technology.
Cylindrical Waveguides and Multi-Junction Solar Cell Investigated for Two-Dimensional Photorecepto-Conversion Scheme
Authors: Wang Yubo , Hong Xingbai , Wu Dan , Wu He , Ishibashi Akira
Published: March 2023

Presented is a new cylindrical waveguide (WG) system based on a two-dimensional photoreceptor-conversion scheme (2DPRCS) for lamppost-type solar-cell systems. The optical properties of polydimethylsiloxane (PDMS) were evaluated as the WG material, and we found that the intrinsic optical loss of our PDMS-based waveguide (~3 m−1) is low enough to be used in a 50 mm diameter lamppost 2DPRCS. The reflection solar concentrator (RSC) is also proposed, which can be combined with multijunction Si solar cells for lamppost-type systems that utilize sunlight coming not only from the south side but also from the east and west sides. We believe that, in the near future, this new approach based on 2DPRCS can enable high-efficiency concentrated photovoltaic systems.
Large-Scale Reconfigurable Integrated Circuits for Wideband Analog Photonic Computing
Authors: Yao Yuhan , Wei Yanxian , Dong Jianji , Li Ming , Zhang Xinliang
Published: March 2023

Photonic integrated circuits (PICs) have been a research hotspot in recent years. Programmable PICs that have the advantages of versatility and reconfigurability that can realize multiple functions through a common structure have been especially popular. Leveraging on-chip couplers and phase shifters, general-purpose waveguide meshes connected in different topologies can be manipulated at run-time and support a variety of applications. However, current waveguide meshes suffer from relatively a low cell amount and limited bandwidth. Here, we demonstrate a reconfigurable photonic integrated computing chip based on a quadrilateral topology network, where typical analog computing functions, including temporal differentiation, integration, and Hilbert transformation, are implemented with a processing bandwidth of up to 40 GHz. By configuring an optical path and changing the splitting ratio of the optical switches in the network, the functions can be switched and the operation order can be tuned. This approach enables wideband analog computing of large-scale PICs in a cost-effective, ultra-compact architecture.
Statistical Analysis of Single-Order Diffraction Grating with Quasi-Random Structures
Authors: Zang Huaping , Cui Zhihao , Wei Lai , Liu Hongjie , Fan Quanping , He Yangfan , Sun Bin , Chen Jihui , Cao Leifeng
Published: March 2023

Single-order diffraction gratings with quasi-random structures are effective optical elements in suppressing harmonics contamination. However, background intensity fluctuations introduced by quasi-random structures may affect the measurement of the spectra and the fluctuations lack quantitative description. A unified theoretical method is provided to describe quasi-random diffraction structures with arbitrary distribution functions and an arbitrary number of microstructures. The effect of the number of microstructures and distribution functions on the level of background fluctuations is evaluated. This work provides important guidance for the design and optimization of single-order diffraction gratings, which are attractive for spectral analysis and monochromator applications in synchrotron beam lines.
Effect of Iron Availability on the Growth and Microcystin Content of Natural Populations of Microcystis spp. from Reservoirs in Central Argentina: A Microcosm Experiment Approach
Authors: Halac Silvana Raquel , Ruibal-Conti Ana Laura , Mengo Luciana del Valle , Ullmer Florencia , Cativa Aldana , Bazan Raquel , Rodriguez Maria Ines
Published: March 2023

The eutrophication of aquatic systems is a problem related to the contribution of excess nutrients—phosphorus (P) and nitrogen (N)—to water bodies, which produces an increase in cyanobacterial blooms. Under eutrophic conditions, P and N concentrations are sufficient for cyanobacteria growth, and some micronutrients are considered to become limiting for population growth. This work aimed to assess the effect of iron on cyanobacteria growth and the content of MCs in natural populations of Microcystis spp. Microcosm setting experiments were carried out with natural samples collected during two bloom events of Microcystis spp., kept under controlled light, temperature and pH conditions. The first bloom sample was exposed to different iron concentrations (400, 700 and 1100 µg Fe·L−1) to determine the optimum concentration for growth. The second was exposed to different iron addition modes (one: T1P, and two pulses: T2P) to imitate the iron increase produced by the downward migration of Microcystis spp. colonies. Our results show that iron is a growth-promoting factor and that its optimal range of concentrations for the growth of Microcystis spp. under the experimental setting conditions is between 700 and 1100 µg Fe·L−1. On the other hand, growth rates were not significantly different between T1P and T2P; thus, different addition modes did not have an effect on growth. Regarding microcystin content, the MC quota in natural populations of Microcystis spp. did not show a clear relationship with the iron supply. This work contributes to the understanding of the underlying factors affecting cyanobacteria bloom formation and the production of MCs, which in turn would impact the development of management strategies to control cyanobacteria blooms.
Detergent-Resistant Membranes in Chloroplasts and Mitochondria of the Halophyte Salicornia perennans under Salt Stress
Authors: Rozentsvet Olga A. , Bogdanova Elena S. , Nurminsky Vadim N. , Nesterov Viktor N. , Chernyshov Michael Yu.
Published: March 2023

Halophytes represent important models for studying the key mechanisms of salt tolerance. One approach to the development of new knowledge of salt tolerance is to study the properties of detergent-resistant membranes (DRMs). In this work, the lipid profiles of DRMs of chloroplasts and mitochondria of euhalophyte Salicornia perennans Willd, before and after their exposure to shock concentrations of NaCl, have been investigated. We found that DRMs of chloroplasts are enriched in cerebrosides (CERs) and that sterols (STs) dominate the mass of mitochondrial DRMs. Also, it has been proven that (i) the impact of salinity provokes obvious growth in the content of CERs in DRMs of chloroplasts; (ii) the content of STs in DRMs of chloroplasts does not change under the influence of NaCl; (iii) salinity also causes some elevation in the content of monounsaturated and saturated fatty acids (FAs). Considering the fact that DRMs represent integral parts of both chloroplast and mitochondrial membranes, the authors have come to the conclusion that the cells of euhalophyte S. perennans, under the impact of salinity, presumes the choice (by the cell) of some specific composition of lipids and FAs in the membrane. This may be considered as a specific protection reaction of the plant cell against salinity.
A Survey of the Transcriptomic Resources in Durum Wheat: Stress Responses, Data Integration and Exploitation
Authors: Zuluaga Diana Lucia , Blanco Emanuela , Mangini Giacomo , Sonnante Gabriella , Curci Pasquale Luca
Published: March 2023

Durum wheat (Triticum turgidum subsp. durum (Desf.) Husn.) is an allotetraploid cereal crop of worldwide importance, given its use for making pasta, couscous, and bulgur. Under climate change scenarios, abiotic (e.g., high and low temperatures, salinity, drought) and biotic (mainly exemplified by fungal pathogens) stresses represent a significant limit for durum cultivation because they can severely affect yield and grain quality. The advent of next-generation sequencing technologies has brought a huge development in transcriptomic resources with many relevant datasets now available for durum wheat, at various anatomical levels, also focusing on phenological phases and environmental conditions. In this review, we cover all the transcriptomic resources generated on durum wheat to date and focus on the corresponding scientific insights gained into abiotic and biotic stress responses. We describe relevant databases, tools and approaches, including connections with other “omics” that could assist data integration for candidate gene discovery for bio-agronomical traits. The biological knowledge summarized here will ultimately help in accelerating durum wheat breeding.
Efficient Somatic Embryogenesis, Regeneration and Acclimatization of Panax ginseng Meyer: True-to-Type Conformity of Plantlets as Confirmed by ISSR Analysis
Authors: Lee Jung-Woo , Kim Jang-Uk , Bang Kyong-Hwan , Kwon Nayeong , Kim Young-Chang , Jo Ick-Hyun , Park Young-Doo
Published: March 2023

Panax ginseng Meyer grows in east Russia and Asia. There is a high demand for this crop due to its medicinal properties. However, its low reproductive efficiency has been a hindrance to the crop’s widespread use. This study aims to establish an efficient regeneration and acclimatization system for the crop. The type of basal media and strength were evaluated for their effects on somatic embryogenesis, germination, and regeneration. The highest rate of somatic embryogenesis was achieved for the basal media MS, N6, and GD, with the optimal nitrogen content (≥35 mM) and NH4+/NO3− ratio (1:2 or 1:4). The full-strength MS medium was the best one for somatic embryo induction. However, the diluted MS medium had a more positive effect on embryo maturation. Additionally, the basal media affected shooting, rooting, and plantlet formation. The germination medium containing 1/2 MS facilitated good shoot development; however, the medium with 1/2 SH yielded outstanding root development. In vitro-grown roots were successfully transferred to soil, and they exhibited a high survival rate (86.3%). Finally, the ISSR marker analysis demonstrated that the regenerated plants were not different from the control. The obtained results provide valuable information for a more efficient micropropagation of various P. ginseng cultivars.
Designing Novel Strategies for Improving Old Legumes: An Overview from Common Vetch
Authors: Ramírez-Parra Elena , De la Rosa Lucía
Published: March 2023

Common vetch (Vicia sativa L.) is a grain legume used in animal feeding, rich in protein content, fatty acid, and mineral composition that makes for a very adequate component to enrich feedstuff. In addition, relevant pharmacological properties have been reported in humans. The common vetch, similar to other legumes, can fix atmospheric nitrogen, a crucial feature for sustainable agricultural systems. These properties enhance the use of vetch as a cover crop and its sowing in intercropping systems. Moreover, several studies have recently pointed out the potential of vetch in the phytoremediation of contaminated soils. These characteristics make vetch a relevant crop, which different potential improvements target. Varieties with different yields, flowering times, shattering resistance, nutritional composition, rhizobacteria associations, drought tolerance, nitrogen fixation capacity, and other agronomic-relevant traits have been identified when different vetch accessions are compared. Recently, the analysis of genomic and transcriptomic data has allowed the development of different molecular markers to be used for assisted breeding purposes, promoting crop improvement. Here, we review the potential of using the variability of V. sativa genetic resources and new biotechnological and molecular tools for selecting varieties with improved traits to be used in sustainable agriculture systems.
Soil Effects on the Bioactivity of Hydroxycoumarins as Plant Allelochemicals
Authors: Facenda Gracia , Real Miguel , Galán-Pérez Jose A. , Gámiz Beatriz , Celis Rafael
Published: March 2023

Soil plays a primary role in the activity of plant allelochemicals in natural and agricultural systems. In this work, we compared the phytotoxicity of three natural hydroxycoumarins (umbelliferone, esculetin, and scopoletin) to different model plant species (Lactuca sativa, Eruca sativa, and Hordeum vulgare) in Petri dishes, and then selected the most phytotoxic compound (umbelliferone) to assess how its adsorption and dissipation in two distinct soils affected the expression of its phytotoxic activity. The root growth inhibitory effect of umbelliferone was significantly greater than that of esculetin and scopoletin, and the dicot species (L. sativa and E. sativa) were more sensitive to the hydroxycoumarins than the monocot species (H. vulgare). For all three plant species tested, the phytotoxicity of umbelliferone decreased in the following order: soilless (Petri dishes) > soil 1 > soil 2. In soil 2 (alkaline), umbelliferone displayed negligible adsorption (Kf < 0.01) and rapid biodegradation (t1/2 = 0.2–0.8 days), and its phytotoxicity was barely expressed. In soil 1 (acid), umbelliferone displayed enhanced adsorption (Kf = 2.94), slower biodegradation (t1/2 = 1.5–2.1 days), and its phytotoxicity was better expressed than in soil 2. When the microbial activity of soil 2 was suppressed by autoclaving, the phytotoxicity of umbelliferone, in the presence of soil, became similar to that observed under soilless conditions. The results illustrate how soil processes can reduce the allelopathic activity of hydroxycoumarins in natural and agricultural ecosystems, and suggest scenarios where the bioactivity of hydroxycoumarins may be better expressed.