bluuebunny/crossref_metadata_2025 huggingface dataset:
Supplementary Figure 5 from MIG-7 Controls COX-2/PGE2-Mediated Lung Cancer Metastasis
Authors: Ho Ming-Yi , Liang Shu-Mei , Hung Shao-Wen , Liang Chi-Ming
Published: April 2023

PDF file - 177K, Knockdown of MIG-7 in PGE2-treated cancer cells reversed the decline in the amount of Akt associated with B55-alpha
Supplementary Figure 3 from MIG-7 Controls COX-2/PGE2-Mediated Lung Cancer Metastasis
Authors: Ho Ming-Yi , Liang Shu-Mei , Hung Shao-Wen , Liang Chi-Ming
Published: April 2023

PDF file - 133K, PGE2 induces beta-catenin/TCF-4/LEF-1 signaling and MIG-7 protein via EP4
Supplementary Figure 1 from Dose-Dense Chemotherapy Improves Mechanisms of Antitumor Immune Response
Authors: Chang Chih-Long , Hsu Yun-Ting , Wu Chao-Chih , Lai Yan-Zen , Wang Connie , Yang Yuh-Cheng , Wu T.-C. , Hung Chien-Fu
Published: April 2023

PDF file - 262K, Supplemental figure 1 demonstrates superior efficacy of dose dense treatment compared to maximum tolerated dose treatment when using single chemotherapeutic agents.
Supplementary Figures 1 - 6, Tables 1 - 6 from Molecular Profiling of Tumor Cells in Cerebrospinal Fluid and Matched Primary Tumors from Metastatic Breast Cancer Patients with Leptomeningeal Carcinomatosis
Authors: Magbanua Mark Jesus M. , Melisko Michelle , Roy Ritu , Sosa Eduardo V. , Hauranieh Louai , Kablanian Andrea , Eisenbud Lauren E. , Ryazantsev Artem , Au Alfred , Scott Janet H. , Park John W.
Published: April 2023

PDF file - 2197K, Methods describing fluorescence in situ hybridization (FISH) analysis in circulating tumor cells (CSFTCs). Case studies involving genomic profiling of tumor cells in the cerebrospinal fluid (CSFTCs) in 7 patients. Fig. S1. Workflow for sample processing. Fig. S2. Study scheme. Fig. S3. Comparison of genomic aberrations between CSFTCs, breast primary tumors, and CTCs. Fig. S4. Comparison of genomic aberrations between CSFTCs and corresponding primary tumors (n=6 pairs). Fig. S5. Genomic profiling of CSFTCs from patients A) 108, B) 112, C) 4014, D) CSF1, E) CSF2, F) CSF5, and G) CSF7. Fig. S6. Serial aCGH profiling of CSFTCs. Table S1. Cell inputs and sample quality control (QC) assessment. Table S2. Mutation screening via Sequenom OncoCarta Panel. Table S3. 64-gene panel for Taqman(TM) Low Density Array (TLDA) RT-PCR analysis. Table S4. Comparison of genomic aberrations between CSFTCs vs. breast primary tumors. Table S5. Comparison of genomic aberrations between CSFTCs vs. CTCs. Table S6. Copy number alterations at the chromosome arm level in CSFTC and corresponding primary tumor (PT), axillary lymph node (LN) tissues or distant metastasis (MET), and circulating tumor cells from peripheral blood (PB).
Supplementary Table 1 from PD-1–Expressing Tumor-Infiltrating T Cells Are a Favorable Prognostic Biomarker in HPV-Associated Head and Neck Cancer
Authors: Badoual Cécile , Hans Stéphane , Merillon Nathalie , Van Ryswick Cordélia , Ravel Patrice , Benhamouda Nadine , Levionnois Emeline , Nizard Mevyn , Si-Mohamed Ali , Besnier Nicolas , Gey Alain , Rotem-Yehudar Rinat , Pere Hélène , Tran Thi , Guerin Coralie L. , Chauvat Anne , Dransart Estelle , Alanio Cécile , Albert Sebastien , Barry Beatrix , Sandoval Federico , Quintin-Colonna Françoise , Bruneval Patrick , Fridman Wolf H. , Lemoine Francois M. , Oudard Stephane , Johannes Ludger , Olive Daniel , Brasnu Daniel , Tartour Eric
Published: April 2023

PDF file - 54K, Patient characteristics
Supplementary Methods and Materials from PD-1–Expressing Tumor-Infiltrating T Cells Are a Favorable Prognostic Biomarker in HPV-Associated Head and Neck Cancer
Authors: Badoual Cécile , Hans Stéphane , Merillon Nathalie , Van Ryswick Cordélia , Ravel Patrice , Benhamouda Nadine , Levionnois Emeline , Nizard Mevyn , Si-Mohamed Ali , Besnier Nicolas , Gey Alain , Rotem-Yehudar Rinat , Pere Hélène , Tran Thi , Guerin Coralie L. , Chauvat Anne , Dransart Estelle , Alanio Cécile , Albert Sebastien , Barry Beatrix , Sandoval Federico , Quintin-Colonna Françoise , Bruneval Patrick , Fridman Wolf H. , Lemoine Francois M. , Oudard Stephane , Johannes Ludger , Olive Daniel , Brasnu Daniel , Tartour Eric
Published: April 2023

PDF file - 81K
Supplementary Table 2 from Identification of Two Distinct Carcinoma-Associated Fibroblast Subtypes with Differential Tumor-Promoting Abilities in Oral Squamous Cell Carcinoma
Authors: Costea Daniela Elena , Hills Allison , Osman Amani H. , Thurlow Johanna , Kalna Gabriela , Huang Xiaohong , Pena Murillo Claudia , Parajuli Himalaya , Suliman Salwa , Kulasekara Keerthi K. , Johannessen Anne Chr. , Partridge Max
Published: April 2023

PDF file - 79K, Supplementary Table ST2. Quantification of movement parameters of CAF-N and NF.
Supplementary Figure 4 from PD-1–Expressing Tumor-Infiltrating T Cells Are a Favorable Prognostic Biomarker in HPV-Associated Head and Neck Cancer
Authors: Badoual Cécile , Hans Stéphane , Merillon Nathalie , Van Ryswick Cordélia , Ravel Patrice , Benhamouda Nadine , Levionnois Emeline , Nizard Mevyn , Si-Mohamed Ali , Besnier Nicolas , Gey Alain , Rotem-Yehudar Rinat , Pere Hélène , Tran Thi , Guerin Coralie L. , Chauvat Anne , Dransart Estelle , Alanio Cécile , Albert Sebastien , Barry Beatrix , Sandoval Federico , Quintin-Colonna Françoise , Bruneval Patrick , Fridman Wolf H. , Lemoine Francois M. , Oudard Stephane , Johannes Ludger , Olive Daniel , Brasnu Daniel , Tartour Eric
Published: April 2023

PDF file - 2.7MB, Correlation between PDL-1 expression and PD-1 positive infiltrating T cells
Supplementary Table 1 from Identification of Two Distinct Carcinoma-Associated Fibroblast Subtypes with Differential Tumor-Promoting Abilities in Oral Squamous Cell Carcinoma
Authors: Costea Daniela Elena , Hills Allison , Osman Amani H. , Thurlow Johanna , Kalna Gabriela , Huang Xiaohong , Pena Murillo Claudia , Parajuli Himalaya , Suliman Salwa , Kulasekara Keerthi K. , Johannessen Anne Chr. , Partridge Max
Published: April 2023

XLSX file - 4536K, Supplementary Excel File ST1. Worksheets summarizing results after RP and DAVIS analysis of gene microarray data.
Supplementary Figures 1 - 8 from Identification of Two Distinct Carcinoma-Associated Fibroblast Subtypes with Differential Tumor-Promoting Abilities in Oral Squamous Cell Carcinoma
Authors: Costea Daniela Elena , Hills Allison , Osman Amani H. , Thurlow Johanna , Kalna Gabriela , Huang Xiaohong , Pena Murillo Claudia , Parajuli Himalaya , Suliman Salwa , Kulasekara Keerthi K. , Johannessen Anne Chr. , Partridge Max
Published: April 2023

PDF file - 2707K, Fig. S1. Phenotypical characterization of CAF, DAF and NF used in the study. Fig. S2. Expression of known gene expression markers of CAFs at mRNA level. Fig. S3. Validation of microarray data at the protein level. Fig. S4. Invasion of transformed human oral keratinocytes in 3D constructs depends on the type of fibroblasts embedded in the collagen biomatrix. Fig. S5. Quantification of growth factor synthesis by CAF and NF maintained in 2D cultures and 3D constructs. Fig. S6. Quantification of RhoA-GTPase and Rac1/2/3-GTPase in CAF-N and NF. Fig. S7. Visualization of the pattern of distribution of hyaluronic acid (HA) in patient samples and 3D constructs with alcian blue staining. Fig. S8. Quantification of HAS2 mRNA levels in HAS2 shRNA (h) lentiviral transduced CAF.
Supplementary Figure Legend from Identification of Two Distinct Carcinoma-Associated Fibroblast Subtypes with Differential Tumor-Promoting Abilities in Oral Squamous Cell Carcinoma
Authors: Costea Daniela Elena , Hills Allison , Osman Amani H. , Thurlow Johanna , Kalna Gabriela , Huang Xiaohong , Pena Murillo Claudia , Parajuli Himalaya , Suliman Salwa , Kulasekara Keerthi K. , Johannessen Anne Chr. , Partridge Max
Published: April 2023

PDF file - 103K
Supplementary Figure Legends from Tumor Cells Upregulate Normoxic HIF-1α in Response to Doxorubicin
Authors: Cao Yiting , Eble Joseph M. , Moon Ejung , Yuan Hong , Weitzel Douglas H. , Landon Chelsea D. , Yu-Chih Nien Charleen , Hanna Gabi , Rich Jeremy N. , Provenzale James M. , Dewhirst Mark W.
Published: April 2023

PDF file, 114K.
Supplementary Figure Legends from Trop-2 Promotes Prostate Cancer Metastasis By Modulating β1 Integrin Functions
Authors: Trerotola Marco , Jernigan Danielle L. , Liu Qin , Siddiqui Javed , Fatatis Alessandro , Languino Lucia R.
Published: April 2023

PDF file - 27K
Supplementary Figure 4 from Synergistic Induction of Adaptive Antitumor Immunity by Codelivery of Antigen with α-Galactosylceramide on Exosomes
Authors: Gehrmann Ulf , Hiltbrunner Stefanie , Georgoudaki Anna-Maria , Karlsson Mikael C. , Näslund Tanja I. , Gabrielsson Susanne
Published: April 2023

PDF file - 32K, Germinal center B cells and Plasma cells, but not Follicular B cells are proliferating in response to aGC-loaded exosomes.
Supplementary Figure 2 from Synergistic Induction of Adaptive Antitumor Immunity by Codelivery of Antigen with α-Galactosylceramide on Exosomes
Authors: Gehrmann Ulf , Hiltbrunner Stefanie , Georgoudaki Anna-Maria , Karlsson Mikael C. , Näslund Tanja I. , Gabrielsson Susanne
Published: April 2023

PDF file - 22K, Early activation of marginal zone B cells following exosome administration.
Supplementary Figure 1 from Synergistic Induction of Adaptive Antitumor Immunity by Codelivery of Antigen with α-Galactosylceramide on Exosomes
Authors: Gehrmann Ulf , Hiltbrunner Stefanie , Georgoudaki Anna-Maria , Karlsson Mikael C. , Näslund Tanja I. , Gabrielsson Susanne
Published: April 2023

PDF file - 38K, aGC-loaded exosomes activate innate immune cells.
Supplementary Figure 3 from Trop-2 Promotes Prostate Cancer Metastasis By Modulating β1 Integrin Functions
Authors: Trerotola Marco , Jernigan Danielle L. , Liu Qin , Siddiqui Javed , Fatatis Alessandro , Languino Lucia R.
Published: April 2023

PDF file - 318K, Surface levels of β1, αv and α5 integrins are not affected by Trop-2.
Supplementary Figure 2 from Trop-2 Promotes Prostate Cancer Metastasis By Modulating β1 Integrin Functions
Authors: Trerotola Marco , Jernigan Danielle L. , Liu Qin , Siddiqui Javed , Fatatis Alessandro , Languino Lucia R.
Published: April 2023

PDF file - 1376K, Trop-2 promotes prostate cancer cell migration on FN.
Supplementary Figures 1 - 10 from Cytotoxic Activity of Tivantinib (ARQ 197) Is Not Due Solely to c-MET Inhibition
Authors: Katayama Ryohei , Aoyama Aki , Yamori Takao , Qi Jie , Oh-hara Tomoko , Song Youngchul , Engelman Jeffrey A. , Fujita Naoya
Published: April 2023

PDF file - 800K, c-Met dependency of the used cell lines (S1); Antitumor activity of tivantinib on c-Met-non-addicted tumor cells (S2); Change in c-Met signaling after exposure to Tivantinib or Crizotinib for 24h (S3); Tivantinib did not inhibit c-MET activation in A549 cells (S4); Apoptosis induction by Tivantinib long time treatment in EBC-1 and MKN45 cells (S5); Tivantinib induces G2/M arrest as similar as tubulin inhibitor vincristine (S6); Growth inhibition against a panel of 39 human cancer cell lines by vincristine (S7); Tivantinib treatment led to a loss of microtubules in EBC1 cells (S8); Tivantinib short time treatment led to a loss of microtubules in A549 and EBC-1 cells (S9); Tivantinib inhibits tubulin polymerization in a dose dependent manner (S10).
Supplementary Table 4 from Novel Oncogenic PDGFRA Mutations in Pediatric High-Grade Gliomas
Authors: Paugh Barbara S. , Zhu Xiaoyan , Qu Chunxu , Endersby Raelene , Diaz Alexander K. , Zhang Junyuan , Bax Dorine A. , Carvalho Diana , Reis Rui M. , Onar-Thomas Arzu , Broniscer Alberto , Wetmore Cynthia , Zhang Jinghui , Jones Chris , Ellison David W. , Baker Suzanne J.
Published: April 2023

XLS file, 11K, PDGFRA sequencing primers.
Supplementary Table 2 from Novel Oncogenic PDGFRA Mutations in Pediatric High-Grade Gliomas
Authors: Paugh Barbara S. , Zhu Xiaoyan , Qu Chunxu , Endersby Raelene , Diaz Alexander K. , Zhang Junyuan , Bax Dorine A. , Carvalho Diana , Reis Rui M. , Onar-Thomas Arzu , Broniscer Alberto , Wetmore Cynthia , Zhang Jinghui , Jones Chris , Ellison David W. , Baker Suzanne J.
Published: April 2023

XLS file, 19K, Clinical sample information, PDGFRA signaling alterations and TP53 mutations.
Supplementary Table 1 from Novel Oncogenic PDGFRA Mutations in Pediatric High-Grade Gliomas
Authors: Paugh Barbara S. , Zhu Xiaoyan , Qu Chunxu , Endersby Raelene , Diaz Alexander K. , Zhang Junyuan , Bax Dorine A. , Carvalho Diana , Reis Rui M. , Onar-Thomas Arzu , Broniscer Alberto , Wetmore Cynthia , Zhang Jinghui , Jones Chris , Ellison David W. , Baker Suzanne J.
Published: April 2023

XLS file, 11K, PDGFRA mutations in pediatric HGG.
Supplementary Figure Legends and Figures from Novel Oncogenic PDGFRA Mutations in Pediatric High-Grade Gliomas
Authors: Paugh Barbara S. , Zhu Xiaoyan , Qu Chunxu , Endersby Raelene , Diaz Alexander K. , Zhang Junyuan , Bax Dorine A. , Carvalho Diana , Reis Rui M. , Onar-Thomas Arzu , Broniscer Alberto , Wetmore Cynthia , Zhang Jinghui , Jones Chris , Ellison David W. , Baker Suzanne J.
Published: April 2023

PDF file, 3383K, Figure-S1: Missense mutation resulting in alternative splicing and RT-PCR screens for KDR-PDGFRA fusion and PDGFRA ; Figure-S2: Analysis of PDGFR� downstream effectors ; Figure-S3: Mutant PDGFR� is sensitive to small molecule inhibitors ; Figure-S4: Dose-dependent sensitivity to crenolanib ; Figure-S5: Crenolanib and desatinib induce G0/G1 arrest ; Figure-S6: Signaling in PDGFRa-driven HGGs ; Figure-S7: PDGFR�-driven murine HGGs show a range of gene expression signatures.
Supplementary Figures from HOXB13 Mediates Tamoxifen Resistance and Invasiveness in Human Breast Cancer by Suppressing ERα and Inducing IL-6 Expression
Authors: Shah Nilay , Jin Kideok , Cruz Leigh-Ann , Park Sunju , Sadik Helen , Cho Soonweng , Goswami Chirayu Pankaj , Nakshatri Harikrishna , Gupta Rajnish , Chang Howard Y. , Zhang Zhe , Cimino-Mathews Ashley , Cope Leslie , Umbricht Christopher , Sukumar Saraswati
Published: April 2023

PDF file, 159K, Figure S1: Characterization of HOXB13 expression in breast cancer cell lines in vitro and in vivo ; Figure S2: HOXB13 and ERα expression in tamoxifen-resistant MCF-7 cell lines, and reversal of tam-resistance by depletion of HOXB13. ; Figure S3: HOXB13 promotes stromal recruitment through expression of IL6.
Supplementary Figure 2 from c-Src Modulates Estrogen-Induced Stress and Apoptosis in Estrogen-Deprived Breast Cancer Cells
Authors: Fan Ping , Griffith Obi L. , Agboke Fadeke A. , Anur Pavana , Zou Xiaojun , McDaniel Russell E. , Creswell Karen , Kim Sung Hoon , Katzenellenbogen John A. , Gray Joe W. , Jordan V. Craig
Published: April 2023

PDF file - 104K, 2A, Activation of pS2 by different concentrations of estrogen dendrimer conjugate (EDC). MCF-7:5C cells were treated with vehicle (0.1% MeOH), different concentrations of EDC, E2, and empty dendrimer as indicated for 8 hours in triplicate. Cells were harvested in TRIzol for real-time PCR. 2B, Cell growth after EDC treatment. MCF-7:5C cells were treated with vehicle (0.1% MeOH), different concentrations of EDC, E2, and empty dendrimer as indicated for 7 days in triplicate. Cells were harvested and total DNA was determined using a DNA fluorescence quantitation kit. 2C, The c-Src inhibitor blocked EDC activated non-genomic pathway. MCF-7:5C cells were treated with vehicle (0.1% MeOH), EDC (10-8 mol/L), PP2 (5x10-6 mol/L), EDC (10-8 mol/L) plus PP2 (5x10-6 mol/L) respectively for 15 minutes and the cell lysates were harvested. Phosphorylated MAPK was examined by immunoblotting with primary antibody. Immunoblotting for total MAPK was used for loading control. 2D, EDC rapidly activated MAPK and c-Src in MCF-7:5C cells. MCF-7:5C cells were treated with vehicle (0.1% MeOH) and EDC (10-8 mol/L) for different time points as indicated and the cell lysates were harvested. Phosphorylated MAPK and c-Src were examined by immunoblotting with primary antibodies. Immunoblotting for total MAPK and c-Src were used for loading controls. 2E, EDC activated signaling pathways after 24 hours. MCF-7:5C cells were treated with vehicle (0.1% MeOH), EDC (10-8 mol/L), PP2 (5x10-6 mol/L), EDC (10-8 mol/L) plus PP2 (5x10-6 mol/L) respectively for 24 hours and 48 hours. Cell lysates were harvested. Phosphorylated MAPK, Akt, and c-Src were examined by immunoblotting with primary antibodies. Immunoblotting for total MAPK, Akt, and c-Src were used for loading controls.
Supplementary Figure 9 from The Exomes of the NCI-60 Panel: A Genomic Resource for Cancer Biology and Systems Pharmacology
Authors: Abaan Ogan D. , Polley Eric C. , Davis Sean R. , Zhu Yuelin J. , Bilke Sven , Walker Robert L. , Pineda Marbin , Gindin Yevgeniy , Jiang Yuan , Reinhold William C. , Holbeck Susan L. , Simon Richard M. , Doroshow James H. , Pommier Yves , Meltzer Paul S.
Published: April 2023

PDF file - 4628K, Volcano plots for 46 cancer genes of interest.
Supplementary Methods from CD95L Cell Surface Cleavage Triggers a Prometastatic Signaling Pathway in Triple-Negative Breast Cancer
Authors: Malleter Marine , Tauzin Sébastien , Bessede Alban , Castellano Rémy , Goubard Armelle , Godey Florence , Levêque Jean , Jézéquel Pascal , Campion Loic , Campone Mario , Ducret Thomas , MacGrogan Gaëtan , Debure Laure , Collette Yves , Vacher Pierre , Legembre Patrick
Published: April 2023

PDF file - 115K
Supplementary Tables 1 - 3, Figures 1 - 2 from Immune Infiltrates Are Prognostic Factors in Localized Gastrointestinal Stromal Tumors
Authors: Rusakiewicz Sylvie , Semeraro Michaela , Sarabi Matthieu , Desbois Mélanie , Locher Clara , Mendez Rosa , Vimond Nadège , Concha Angel , Garrido Federico , Isambert Nicolas , Chaigneau Loic , Le Brun-Ly Valérie , Dubreuil Patrice , Cremer Isabelle , Caignard Anne , Poirier-Colame Vichnou , Chaba Kariman , Flament Caroline , Halama Niels , Jäger Dirk , Eggermont Alexander , Bonvalot Sylvie , Commo Frédéric , Terrier Philippe , Opolon Paule , Emile Jean-François , Coindre Jean-Michel , Kroemer Guido , Chaput Nathalie , Le Cesne Axel , Blay Jean-Yves , Zitvogel Laurence
Published: April 2023

PDF file - 197K, Supplemental Table 1. Characteristics of GIST patients for the immunohistochemistry study. Supplemental Table 2. Characteristics of GIST and STS patients studied in flow cytometry. Supplemental Table 3: Antibodies for flow cytometry and IHC. Supplemental Figure 1. Correlation between T and NK cell infiltrates and the 3-digit Miettinen score. Supplemental Figure 2. GIST TIls are mainly composed of CD4+ T cells.
Supplementary Figure 3 from Nitric Oxide Production Upregulates Wnt/β-Catenin Signaling by Inhibiting Dickkopf-1
Authors: Du Qiang , Zhang Xinglu , Liu Quan , Zhang Xianghong , Bartels Christian E. , Geller David A.
Published: April 2023

PDF file - 136K, iNOS/NO downregulated DKK1 gene expression in cancer cells. A, HCT116 and MCF7 cell lines were treated with NO donor SNAP (500 microM) for 4 hrs. Total RNA was isolated for RT-PCR analysis. B, SW480-DKK1 and SW480-vector (control) cell lines were incubated for 24 hrs and total proteins were isolated for Western blot analysis with the indicated antibodies. C, SW480-DKK1 and SW480-vector cell lines were transfected with pTOP/pFOP for 48 hrs, and the cell lysates were used for luciferase reporter assay. D, SW480-iNOS-DKK1and SW480-iNOS-vector cell lines were incubated for 24 hours. Total proteins were collected for Western blot analysis with iNOS and DKK1 antibodies. E, HCT116 cells were transfected with pCDNA3-hiNOS and pCDNA3-vector, and with pcDNA3.1/V5-His-hDDK1 and pCDNA3.1/V5-His-vector. After 36 hours, total protein was isolated for immunoblotting with c-MYC or Beta-actin antibody.
Supplementary Figures from Rapid Induction of Lung Adenocarcinoma by Fibroblast Growth Factor 9 Signaling through FGF Receptor 3
Authors: Yin Yongjun , Betsuyaku Tomoko , Garbow Joel R. , Miao Jinbai , Govindan Ramaswamy , Ornitz David M.
Published: April 2023

PDF file, 3363K, Expression of eGFP and Fgf9 following doxycycline induction for four days (S1); FGF9 promotes proliferation of cells in the bronchioalveolar duct junction (S2); Co-expression of FGFR3 and Sca-1 in the bronchioalveolar duct junction following induction of FGF9 (S3).
Supplementary Table 1 from A Classification Model for BRCA2 DNA Binding Domain Missense Variants Based on Homology-Directed Repair Activity
Authors: Guidugli Lucia , Pankratz Vernon S. , Singh Namit , Thompson James , Erding Catherine A. , Engel Christoph , Schmutzler Rita , Domchek Susan , Nathanson Katherine , Radice Paolo , Singer Christian , Tonin Patricia N. , Lindor Noralane M. , Goldgar David E. , Couch Fergus J.
Published: April 2023

PDF file - 66K, Table showing the normalized mean HDR assay fold-change and log transformed values for each VUS
Supplementary Methods, Figure Legends, Tables 1 - 2 from miR-186 Downregulation Correlates with Poor Survival in Lung Adenocarcinoma, Where It Interferes with Cell-Cycle Regulation
Authors: Cai Junchao , Wu Jueheng , Zhang Huizhong , Fang Lishan , Huang Yongbo , Yang Yi , Zhu Xun , Li Rong , Li Mengfeng
Published: April 2023

PDF file - 182K, Clinicopathologic characteristics of patients enrolled for sample examination and expression of miR-186 in lung cancer, Correlation between the clinical pathologic features and expression of miR-186 levels
Supplementary Figure 2 from miR-186 Downregulation Correlates with Poor Survival in Lung Adenocarcinoma, Where It Interferes with Cell-Cycle Regulation
Authors: Cai Junchao , Wu Jueheng , Zhang Huizhong , Fang Lishan , Huang Yongbo , Yang Yi , Zhu Xun , Li Rong , Li Mengfeng
Published: April 2023

PDF file - 24K, Real-time analysis of miR-186 expression in A549 and SK-MES-1 cells transfected with anti-miR-186 (Anti-miR) or NC oligonucleotides
Supplementary Figure 1 from miR-205 Targets PTEN and PHLPP2 to Augment AKT Signaling and Drive Malignant Phenotypes in Non–Small Cell Lung Cancer
Authors: Cai Junchao , Fang Lishan , Huang Yongbo , Li Rong , Yuan Jie , Yang Yi , Zhu Xun , Chen Baixue , Wu Jueheng , Li Mengfeng
Published: April 2023

PDF file, 32K, Real-time analysis of miR-205 expression in normal lung epithelial cells (NLEs), immortalized human bronchial epithelial cell line (BEAS-2B) and several NSCLC cell lines.
Supplementary Methods from Constitutive β-Catenin Activation Induces Male-Specific Tumorigenesis in the Bladder Urothelium
Authors: Lin Congxing , Yin Yan , Stemler Kristina , Humphrey Peter , Kibel Adam S. , Mysorekar Indira U. , Ma Liang
Published: April 2023

PDF file, 27K.
Supplementary Figures from Constitutive β-Catenin Activation Induces Male-Specific Tumorigenesis in the Bladder Urothelium
Authors: Lin Congxing , Yin Yan , Stemler Kristina , Humphrey Peter , Kibel Adam S. , Mysorekar Indira U. , Ma Liang
Published: April 2023

PDF file, 3548K, Supplemental Fig. 1 Phenotype of B-catenin GOF mutants 12 weeks after doxycycline treatment. ; Supplemental Fig. 2 Phenotypic comparison of GOF males and females. ; Supplemental Fig. 3 Western blot analyses showing equal B-catenin expression in GOF males and females. ; Supplemental Fig. 4 Differential expression of marker genes revealing distinct cell types in the adult mouse urothelium. ; Supplemental Fig. 5 Enhanced cell proliferation in the GOF mouse urothelia. ; Supplemental Fig. 6 Ectopic B-catenin activation inducing basal urothelial proliferation. ; Supplemental Fig. 7 Histopathology of GOF urothelia 5weeks after starting Dox treatment. ; Supplemental Fig. 8 Nuclear AR expression demarcating the dysplastic/tumor epithelium. ; Supplemental Fig. 9 AR expression in the UMUC3 cells. Supplemental Fig. 10 Expression of regulatory genes assayed by real time RT-PCR. ; Supplemental Fig. 10 Real time RT-PCR using urothelial cells isolated from animals of different genotypes and sexes indicated.
Supplementary Figures 1 - 4, Tables 1 - 2 from Loss of TBK1 Induces Epithelial–Mesenchymal Transition in the Breast Cancer Cells by ERα Downregulation
Authors: Yang Kyung-Min , Jung YunShin , Lee Jeong-Mi , Kim WonJoo , Cho Jin Ki , Jeong Joon , Kim Seong-Jin
Published: April 2023

PDF file - 1706K, Supplementary Fig. S1. TBK1 knockdown increases cell migration. Supplementary Fig. S2. TBK1 knockdown increases cell invasion. Supplementary Fig. S3. TBK1 knockdown did not inhibit the expression of ERalpha proteins via the proteasomal degradation pathway. Supplementary Fig. S4. TBK1 knockdown induces phosphorylation of AKT in ERalpha-positive breast cancer cells. Supplementary Table 1. ERalpha promoter-specific primer pairs. Supplementary Table 2. Sequence of putative FOXO3A binding site in the 4-kb ERalpha promoter.
Supplementary Figure Legend from A Transcriptional and Metabolic Signature of Primary Aneuploidy Is Present in Chromosomally Unstable Cancer Cells and Informs Clinical Prognosis
Authors: Sheltzer Jason M.
Published: April 2023

PDF file - 67K
Supplementary Figure 7 from Carbon Monoxide Expedites Metabolic Exhaustion to Inhibit Tumor Growth
Authors: Wegiel Barbara , Gallo David , Csizmadia Eva , Harris Clair , Belcher John , Vercellotti Gregory M. , Penacho Nuno , Seth Pankaj , Sukhatme Vikas , Ahmed Asif , Pandolfi Pier Paolo , Helczynski Leszek , Bjartell Anders , Persson Jenny Liao , Otterbein Leo E.
Published: April 2023

PDF file - 797K, Augmentation of doxorubicin-induced cell death of PC3 cells by CO is dependent in part on reactive oxygen species (ROS) generation.
Supplementary Figure 8 from A Transcriptional and Metabolic Signature of Primary Aneuploidy Is Present in Chromosomally Unstable Cancer Cells and Informs Clinical Prognosis
Authors: Sheltzer Jason M.
Published: April 2023

PDF file - 724K, Kaplan-Meier analysis using the HET5 gene set.
Supplementary Figure 6 from Carbon Monoxide Expedites Metabolic Exhaustion to Inhibit Tumor Growth
Authors: Wegiel Barbara , Gallo David , Csizmadia Eva , Harris Clair , Belcher John , Vercellotti Gregory M. , Penacho Nuno , Seth Pankaj , Sukhatme Vikas , Ahmed Asif , Pandolfi Pier Paolo , Helczynski Leszek , Bjartell Anders , Persson Jenny Liao , Otterbein Leo E.
Published: April 2023

PDF file - 1394K, Effects of CO on metabolic gene expression.
Supplementary Figure 7 from A Transcriptional and Metabolic Signature of Primary Aneuploidy Is Present in Chromosomally Unstable Cancer Cells and Informs Clinical Prognosis
Authors: Sheltzer Jason M.
Published: April 2023

PDF file - 486K, Classification similarities between PCNA25, CIN70, and TRI70.
Supplementary Figure 6 from A Transcriptional and Metabolic Signature of Primary Aneuploidy Is Present in Chromosomally Unstable Cancer Cells and Informs Clinical Prognosis
Authors: Sheltzer Jason M.
Published: April 2023

PDF file - 578K, Patient stratification in 27 tumor gene expression cohorts.
Supplementary Figure 5 from Carbon Monoxide Expedites Metabolic Exhaustion to Inhibit Tumor Growth
Authors: Wegiel Barbara , Gallo David , Csizmadia Eva , Harris Clair , Belcher John , Vercellotti Gregory M. , Penacho Nuno , Seth Pankaj , Sukhatme Vikas , Ahmed Asif , Pandolfi Pier Paolo , Helczynski Leszek , Bjartell Anders , Persson Jenny Liao , Otterbein Leo E.
Published: April 2023

PDF file - 745K, Effects of CO on mitochondrial membrane potential.
Supplementary Figure 4 from Carbon Monoxide Expedites Metabolic Exhaustion to Inhibit Tumor Growth
Authors: Wegiel Barbara , Gallo David , Csizmadia Eva , Harris Clair , Belcher John , Vercellotti Gregory M. , Penacho Nuno , Seth Pankaj , Sukhatme Vikas , Ahmed Asif , Pandolfi Pier Paolo , Helczynski Leszek , Bjartell Anders , Persson Jenny Liao , Otterbein Leo E.
Published: April 2023

PDF file - 8322K, Effects of CO on markers of proliferation and invasion.
Supplementary Figure 3 from A Transcriptional and Metabolic Signature of Primary Aneuploidy Is Present in Chromosomally Unstable Cancer Cells and Informs Clinical Prognosis
Authors: Sheltzer Jason M.
Published: April 2023

PDF file - 395K, Transcriptional similarities between chromosomally-unstable cancer cell lines, trisomic MEFs, chromosomally-unstable MEFs, and cancer cells that have received extra chromosomes via MMCT.
Supplementary Figure 1 from Carbon Monoxide Expedites Metabolic Exhaustion to Inhibit Tumor Growth
Authors: Wegiel Barbara , Gallo David , Csizmadia Eva , Harris Clair , Belcher John , Vercellotti Gregory M. , Penacho Nuno , Seth Pankaj , Sukhatme Vikas , Ahmed Asif , Pandolfi Pier Paolo , Helczynski Leszek , Bjartell Anders , Persson Jenny Liao , Otterbein Leo E.
Published: April 2023

PDF file - 6972K, Carbon monoxide accelerates camptothecin-induced apoptosis via inhibition of Gadd45α and independent of p53.
Supplementary Table 4 from Loss of p120-Catenin Induces Metastatic Progression of Breast Cancer by Inducing Anoikis Resistance and Augmenting Growth Factor Receptor Signaling
Authors: Schackmann Ron C.J. , Klarenbeek Sjoerd , Vlug Eva J. , Stelloo Suzan , van Amersfoort Miranda , Tenhagen Milou , Braumuller Tanya M. , Vermeulen Jeroen F. , van der Groep Petra , Peeters Ton , van der Wall Elsken , van Diest Paul J. , Jonkers Jos , Derksen Patrick W.B.
Published: April 2023

PDF - 82K, Loss of p120 induces secretion of pro-inflammatory cytokines.
Supplementary Methods from Angiopoietin-2 Functions as a Tie2 Agonist in Tumor Models, Where It Limits the Effects of VEGF Inhibition
Authors: Daly Christopher , Eichten Alexandra , Castanaro Carla , Pasnikowski Elizabeth , Adler Alexander , Lalani Alshad S. , Papadopoulos Nicholas , Kyle Alastair H. , Minchinton Andrew I. , Yancopoulos George D. , Thurston Gavin
Published: April 2023

PDF file - 115K, Detailed methods for molecular characterization of REGN910, tumor histology, tumor perfusion and real-time PCR
Supplementary Figure Legends 1-4 from Angiopoietin-2 Functions as a Tie2 Agonist in Tumor Models, Where It Limits the Effects of VEGF Inhibition
Authors: Daly Christopher , Eichten Alexandra , Castanaro Carla , Pasnikowski Elizabeth , Adler Alexander , Lalani Alshad S. , Papadopoulos Nicholas , Kyle Alastair H. , Minchinton Andrew I. , Yancopoulos George D. , Thurston Gavin
Published: April 2023

PDF file - 76K
Supplementary Table 2 from Loss of p120-Catenin Induces Metastatic Progression of Breast Cancer by Inducing Anoikis Resistance and Augmenting Growth Factor Receptor Signaling
Authors: Schackmann Ron C.J. , Klarenbeek Sjoerd , Vlug Eva J. , Stelloo Suzan , van Amersfoort Miranda , Tenhagen Milou , Braumuller Tanya M. , Vermeulen Jeroen F. , van der Groep Petra , Peeters Ton , van der Wall Elsken , van Diest Paul J. , Jonkers Jos , Derksen Patrick W.B.
Published: April 2023

PDF - 51K, Correlation of p120 expression with clinicopathological and molecular features of invasive breast cancer.
Supplementary Figure 4 from Angiopoietin-2 Functions as a Tie2 Agonist in Tumor Models, Where It Limits the Effects of VEGF Inhibition
Authors: Daly Christopher , Eichten Alexandra , Castanaro Carla , Pasnikowski Elizabeth , Adler Alexander , Lalani Alshad S. , Papadopoulos Nicholas , Kyle Alastair H. , Minchinton Andrew I. , Yancopoulos George D. , Thurston Gavin
Published: April 2023

PDF file - 292K, Histological analysis of the effect of REGN910 + ZALTRAP� combination treatment on endothelial cell death
Supplementary Materials and Methods from Loss of p120-Catenin Induces Metastatic Progression of Breast Cancer by Inducing Anoikis Resistance and Augmenting Growth Factor Receptor Signaling
Authors: Schackmann Ron C.J. , Klarenbeek Sjoerd , Vlug Eva J. , Stelloo Suzan , van Amersfoort Miranda , Tenhagen Milou , Braumuller Tanya M. , Vermeulen Jeroen F. , van der Groep Petra , Peeters Ton , van der Wall Elsken , van Diest Paul J. , Jonkers Jos , Derksen Patrick W.B.
Published: April 2023

PDF - 96K
Supplementary Figure 1 from Angiopoietin-2 Functions as a Tie2 Agonist in Tumor Models, Where It Limits the Effects of VEGF Inhibition
Authors: Daly Christopher , Eichten Alexandra , Castanaro Carla , Pasnikowski Elizabeth , Adler Alexander , Lalani Alshad S. , Papadopoulos Nicholas , Kyle Alastair H. , Minchinton Andrew I. , Yancopoulos George D. , Thurston Gavin
Published: April 2023

PDF file - 1.6MB, Histological analysis of the effects of REGN910 on endothelial cell death, endothelial cell proliferation and tumor hypoxia
Supplementary Figure 6 from Loss of p120-Catenin Induces Metastatic Progression of Breast Cancer by Inducing Anoikis Resistance and Augmenting Growth Factor Receptor Signaling
Authors: Schackmann Ron C.J. , Klarenbeek Sjoerd , Vlug Eva J. , Stelloo Suzan , van Amersfoort Miranda , Tenhagen Milou , Braumuller Tanya M. , Vermeulen Jeroen F. , van der Groep Petra , Peeters Ton , van der Wall Elsken , van Diest Paul J. , Jonkers Jos , Derksen Patrick W.B.
Published: April 2023

PDF - 828K, p120 knockout sensitizes tumor cells to growth factor signaling.
Supplementary Figure 4 from Loss of p120-Catenin Induces Metastatic Progression of Breast Cancer by Inducing Anoikis Resistance and Augmenting Growth Factor Receptor Signaling
Authors: Schackmann Ron C.J. , Klarenbeek Sjoerd , Vlug Eva J. , Stelloo Suzan , van Amersfoort Miranda , Tenhagen Milou , Braumuller Tanya M. , Vermeulen Jeroen F. , van der Groep Petra , Peeters Ton , van der Wall Elsken , van Diest Paul J. , Jonkers Jos , Derksen Patrick W.B.
Published: April 2023

PDF - 215K, Loss of AJ integrity sensitizes cells to HGF signaling.
Supplementary Figure Legend from Targeting Tumor-Infiltrating Macrophages Decreases Tumor-Initiating Cells, Relieves Immunosuppression, and Improves Chemotherapeutic Responses
Authors: Mitchem Jonathan B. , Brennan Donal J. , Knolhoff Brett L. , Belt Brian A. , Zhu Yu , Sanford Dominic E. , Belaygorod Larisa , Carpenter Danielle , Collins Lynne , Piwnica-Worms David , Hewitt Stephen , Udupi Girish Mallya , Gallagher William M. , Wegner Craig , West Brian L. , Wang-Gillam Andrea , Goedegebuure Peter , Linehan David C. , DeNardo David G.
Published: April 2023

PDF file - 73K
Supplementary Figure 2 from Loss of p120-Catenin Induces Metastatic Progression of Breast Cancer by Inducing Anoikis Resistance and Augmenting Growth Factor Receptor Signaling
Authors: Schackmann Ron C.J. , Klarenbeek Sjoerd , Vlug Eva J. , Stelloo Suzan , van Amersfoort Miranda , Tenhagen Milou , Braumuller Tanya M. , Vermeulen Jeroen F. , van der Groep Petra , Peeters Ton , van der Wall Elsken , van Diest Paul J. , Jonkers Jos , Derksen Patrick W.B.
Published: April 2023

PDF - 4684K, p120 knockdown decreases AJ member expression levels and disrupts cell-cell contact in breast cancer cells.
Supplementary Tables 1 - 5, Figures 1 - 11 from Posttranscriptional Regulation of PER1 Underlies the Oncogenic Function of IREα
Authors: Pluquet Olivier , Dejeans Nicolas , Bouchecareilh Marion , Lhomond Stephanie , Pineau Raphael , Higa Arisa , Delugin Maylis , Combe Chantal , Loriot Sandrine , Cubel Gaelle , Dugot-Senant Nathalie , Vital Anne , Loiseau Hugues , Gosline Sara J.C. , Taouji Said , Hallett Michael , Sarkaria Jann N. , Anderson Keith , Wu Wenting , Rodriguez Fausto J. , Rosenbaum Jean , Saltel Frédéric , Fernandez-Zapico Martin E. , Chevet Eric
Published: April 2023

PDF file - 809K, Table S1 List of genes deregulated in IRE1_DN cells. Table S2 Potential IRE1 cleavage sites on PER1 mRNA. Table S3 Characteristics of human glioma biopsies used in this study. Table S4 Primer Pairs used for PCR and qPCR studies. Table S5 siRNA sequences. Figure S1 PER1 mRNA expression in IRE1 signaling deficient cells. Figure S2 Validation of ski2, xrn1/2 siRNA efficacy. Figure S3 Potential IRE1 cleavage sites on PER1 mRNA. Figure S4 Expression of PER1 mRNA in PER1 silenced cells. Figure S5 XBP1s expression in glioma biopsies by immunohistochemistry. Figure S6: stress independent regulation of PER1 by IRE1α. Figure S7: (A) Two-dimensional modeling of potential Per1 mRNA cleavage sites using the M-Fold program. (B) In vitro RNA cleavage assay. Figure S8: Expression of Per1 mRNA in U87 cells upon lentiviral transduction. Figure S9: determination of protein (A-D) and mRNA (E) expression levels of potential PER1 targets in EV and IRE_DN cells transduced or not with shPER1. Figure S10: impact of CXCL3 re-expression on cell proliferation and neurosphere formation in IRE1_DN cells. Figure S11: (A-F) Immunohistochemical analysis of 3 typical glioblastoma paraffin sections using anti sXBP1 antibodies.
Supplementary Methods from Therapeutic Destruction of Insulin Receptor Substrates for Cancer Treatment
Authors: Reuveni Hadas , Flashner-Abramson Efrat , Steiner Lilach , Makedonski Kirill , Song Renduo , Shir Alexei , Herlyn Meenhard , Bar-Eli Menashe , Levitzki Alexander
Published: April 2023

PDF file - 102K, Additional data regarding experimental procedures, kinetic experiments and anti-tumor activity of NT 157.
Supplementary Figure Legend from Rb1 Haploinsufficiency Promotes Telomere Attrition and Radiation-Induced Genomic Instability
Authors: Gonzalez-Vasconcellos Iria , Anastasov Natasa , Sanli-Bonazzi Bahar , Klymenko Olena , Atkinson Michael J. , Rosemann Michael
Published: April 2023

PDF file - 131K
Supplementary Figure 4 from Tid1-L Inhibits EGFR Signaling in Lung Adenocarcinoma by Enhancing EGFR Ubiquitinylation and Degradation
Authors: Chen Chi-Yuan , Jan Chia-Ing , Lo Jeng-Fan , Yang Shuenn-Chen , Chang Yih-Leong , Pan Szu-Hua , Wang Wen-Lung , Hong Tse-Ming , Yang Pan-Chyr
Published: April 2023

PDF file - 98K, Effect of stable Tid1-L overexpression on the level of EGFR.
Supplementary Figure 3 from Tid1-L Inhibits EGFR Signaling in Lung Adenocarcinoma by Enhancing EGFR Ubiquitinylation and Degradation
Authors: Chen Chi-Yuan , Jan Chia-Ing , Lo Jeng-Fan , Yang Shuenn-Chen , Chang Yih-Leong , Pan Szu-Hua , Wang Wen-Lung , Hong Tse-Ming , Yang Pan-Chyr
Published: April 2023

PDF file - 128K, Subcellular distribution of endogenous and exogenously expressed Tid1.
Supplementary Table 2 from Distinct FAK Activities Determine Progenitor and Mammary Stem Cell Characteristics
Authors: Luo Ming , Zhao Xiaofeng , Chen Song , Liu Suling , Wicha Max S. , Guan Jun-Lin
Published: April 2023

PDF file, 81K, Outgrowth of unsorted MaECs isolated from Cre Ctrl-GFP, Ctrl-GFP and MFCKO-GFP mice.
Supplementary Table 1 from Distinct FAK Activities Determine Progenitor and Mammary Stem Cell Characteristics
Authors: Luo Ming , Zhao Xiaofeng , Chen Song , Liu Suling , Wicha Max S. , Guan Jun-Lin
Published: April 2023

PDF file, 80K, Outgrowth from Ctrl (FAKf/f) MaECs infected with Ad-LacZ or Ad-Cre.
Supplementary Figure 1 from Distinct FAK Activities Determine Progenitor and Mammary Stem Cell Characteristics
Authors: Luo Ming , Zhao Xiaofeng , Chen Song , Liu Suling , Wicha Max S. , Guan Jun-Lin
Published: April 2023

PDF file, 82K, Characterization of distinct cellular populations for expression of luminal marker CK8 and basal marker CK5 using immmunofluorescence.
Supplementary Figure Legend from Myeloid-Derived Suppressor Cells Function as Novel Osteoclast Progenitors Enhancing Bone Loss in Breast Cancer
Authors: Sawant Anandi , Deshane Jessy , Jules Joel , Lee Carnella M. , Harris Brittney A. , Feng Xu , Ponnazhagan Selvarangan
Published: April 2023

PDF file - 128K
Supplementary Figure 7 from Myeloid-Derived Suppressor Cells Function as Novel Osteoclast Progenitors Enhancing Bone Loss in Breast Cancer
Authors: Sawant Anandi , Deshane Jessy , Jules Joel , Lee Carnella M. , Harris Brittney A. , Feng Xu , Ponnazhagan Selvarangan
Published: April 2023

PDF file - 205K, Western blot analysis of HIF-1α, ERK and PI3K in MDSC from different tissue sources
Supplementary Figure 2 from Myeloid-Derived Suppressor Cells Function as Novel Osteoclast Progenitors Enhancing Bone Loss in Breast Cancer
Authors: Sawant Anandi , Deshane Jessy , Jules Joel , Lee Carnella M. , Harris Brittney A. , Feng Xu , Ponnazhagan Selvarangan
Published: April 2023

PDF file - 372K, MDSC(+bone mets) was the only population that differentiated into osteoclasts
Supplementary Figure 1 from eIF4B Phosphorylation by Pim Kinases Plays a Critical Role in Cellular Transformation by Abl Oncogenes
Authors: Yang Jianling , Wang Jun , Chen Ke , Guo Guijie , Xi Ruijiao , Rothman Paul B. , Whitten Douglas , Zhang Lianfeng , Huang Shile , Chen Ji-Long
Published: April 2023

PDF - 21K, Pim-3 has little effect on eIF4B phosphorylation at Ser422.
Supplementary Figure 1 from Myeloid-Derived Suppressor Cells Function as Novel Osteoclast Progenitors Enhancing Bone Loss in Breast Cancer
Authors: Sawant Anandi , Deshane Jessy , Jules Joel , Lee Carnella M. , Harris Brittney A. , Feng Xu , Ponnazhagan Selvarangan
Published: April 2023

PDF file - 143K, Isolation and characterization of MDSC from murine breast cancer model
Supplementary Tables from Infiltrating Macrophages Promote Prostate Tumorigenesis via Modulating Androgen Receptor-Mediated CCL4–STAT3 Signaling
Authors: Fang Lei-Ya , Izumi Kouji , Lai Kuo-Pao , Liang Liang , Li Lei , Miyamoto Hiroshi , Lin Wen-Jye , Chang Chawnshang
Published: April 2023

PDF file, 65K, Table S1. CCL4 expression in prostate tissue microarrays. Table S2. CCL4 expression in prostatic adenocarcinoma and pathologic profile.
Supplementary Materials and Methods from Infiltrating Macrophages Promote Prostate Tumorigenesis via Modulating Androgen Receptor-Mediated CCL4–STAT3 Signaling
Authors: Fang Lei-Ya , Izumi Kouji , Lai Kuo-Pao , Liang Liang , Li Lei , Miyamoto Hiroshi , Lin Wen-Jye , Chang Chawnshang
Published: April 2023

PDF file, 100K.
Supplementary Methods, Figures 1-5 from Novel DNA Damage Checkpoints Mediating Cell Death Induced by the NEDD8-Activating Enzyme Inhibitor MLN4924
Authors: Blank Jonathan L. , Liu Xiaozhen J. , Cosmopoulos Katherine , Bouck David C. , Garcia Khristofer , Bernard Hugues , Tayber Olga , Hather Greg , Liu Ray , Narayanan Usha , Milhollen Michael A. , Lightcap Eric S.
Published: April 2023

PDF file - 156K
Supplementary Materials and Methods and Supplementary Figures 1-10 from Visualizing Inhibition of Nucleosome Mobility and Transcription by Cisplatin–DNA Interstrand Crosslinks in Live Mammalian Cells
Authors: Zhu Guangyu , Song Lina , Lippard Stephen J.
Published: April 2023

PDF - 1937K, Supplementary Materials and Methods. Supplementary Figure 1: Sequences of the 21-mer/33-mer duplexes containing site-specific platinum lesions for the construction of 146-bp duplexes. Supplementary Figure 2: Autoradiograph of a small DNA duplex containing a cisplatin ICL. The oligonucleotides were 5'-end labeled and revealed on a 12 denaturing polyacrylamide gel. Supplementary Figure 3: Autoradiograph of purified 63-mer, 57-mer, 62-mer and 56-mer. An 8% denaturing PAGE was used. Supplementary Figure 4: Sequences of the 63-mer, 62-mer, 57-mer and 56-mer.Supplementary Figure 5: (A) Strategy of synthesis of the 146-bp duplexes; (B) Schematic representation of 146-bp duplexes containing site-specific platinum damage. The sites of the radiolabels are marked by asterisks. The 21-mer/33-mer duplexes are denoted by yellow/blue bars, respectively. Supplementary Figure 6: Autoradiograph of 146-bp duplexes containing site-specific platinum damage. Supplementary Figure 7: Purification of native oligonucleosomes from HeLa cells after FPLC. Supplementary Figure 8: Product distribution after histone transfer reactions onto cis-{Pt(NH3)2}2+ ICL and No Pt duplexes. A 4.5% native polyacrylamide gel was used.Supplementary Figure 9: Autoradiograph of NCPs containing cis-{Pt(NH3)2}2+ ICL, cis-{Pt(NH3)2}2+ 1,2- d(GpG), or No Pt. NCPs were heat shifted at 45 degrees C. A 4.5% native polyacrylamide gel was used. Supplementary Figure 10: Restriction analysis of pGLuc9temG+IS and pGLuc9temG+ICL with BspQI.
Supplementary Figure 1 from Novel DNA Damage Checkpoints Mediating Cell Death Induced by the NEDD8-Activating Enzyme Inhibitor MLN4924
Authors: Blank Jonathan L. , Liu Xiaozhen J. , Cosmopoulos Katherine , Bouck David C. , Garcia Khristofer , Bernard Hugues , Tayber Olga , Hather Greg , Liu Ray , Narayanan Usha , Milhollen Michael A. , Lightcap Eric S.
Published: April 2023

PDF file - 1.6MB, Full Length Western Blots in Support of Figures 4 and 5
Supplementary Figure 3 from SOX10 Ablation Arrests Cell Cycle, Induces Senescence, and Suppresses Melanomagenesis
Authors: Cronin Julia C. , Watkins-Chow Dawn E. , Incao Art , Hasskamp Joanne H. , Schönewolf Nicola , Aoude Lauren G. , Hayward Nicholas K. , Bastian Boris C. , Dummer Reinhard , Loftus Stacie K. , Pavan William J.
Published: April 2023

PDF file, 173K, Transient transfection of SOX10-specific siRNAs in 0380-MMU and UACC 1022 cells.
Supplementary Figures 1 - 4 from The Steroid Receptor Coactivator-3 Is Required for the Development of Castration-Resistant Prostate Cancer
Authors: Tien Jean C-Y. , Liu Zhaoliang , Liao Lan , Wang Fen , Xu Yixiang , Wu Ye-Lin , Zhou Niya , Ittmann Michael , Xu Jianming
Published: April 2023

PDF file - 410K, Supplementary Figure S1. SRC-3 knockout in prostate epithelial cells does not affect prostate morphogenesis during development. Supplementary Figure S2. Deletion of SRC-3 has no impact on tumor histology or cellular proliferation in non-castrated 12-week-old mice. Supplementary Figure S3. Immunohistochemistry for pRPS6 in prostate tumors of castrated PtenCKO and Pten3CKO mice. Supplementary Figure S4. SRC-3 deletion does not change AR protein level in tissue lysate and AR target genes.
Supplementary Table 1 from Comparative Expression Analysis Reveals Lineage Relationships between Human and Murine Gliomas and a Dominance of Glial Signatures during Tumor Propagation In Vitro
Authors: Henriquez Nico V. , Forshew Tim , Tatevossian Ruth , Ellis Matthew , Richard-Loendt Angela , Rogers Hazel , Jacques Thomas S. , Reitboeck Pablo Garcia , Pearce Kerra , Sheer Denise , Grundy Richard G. , Brandner Sebastian
Published: April 2023

XLSX file, 433K, 2500 2-fold differentially expressed in vitro vs in vivo genes.
Supplementary Methods and Figure Legends from Comparative Expression Analysis Reveals Lineage Relationships between Human and Murine Gliomas and a Dominance of Glial Signatures during Tumor Propagation In Vitro
Authors: Henriquez Nico V. , Forshew Tim , Tatevossian Ruth , Ellis Matthew , Richard-Loendt Angela , Rogers Hazel , Jacques Thomas S. , Reitboeck Pablo Garcia , Pearce Kerra , Sheer Denise , Grundy Richard G. , Brandner Sebastian
Published: April 2023

PDF file - 112K
Supplementary Figure 7 from Comparative Expression Analysis Reveals Lineage Relationships between Human and Murine Gliomas and a Dominance of Glial Signatures during Tumor Propagation In Vitro
Authors: Henriquez Nico V. , Forshew Tim , Tatevossian Ruth , Ellis Matthew , Richard-Loendt Angela , Rogers Hazel , Jacques Thomas S. , Reitboeck Pablo Garcia , Pearce Kerra , Sheer Denise , Grundy Richard G. , Brandner Sebastian
Published: April 2023

PDF file, 736K, Derivation of a human ATRT-specific gene set.
Supplementary Methods from Single-Molecule Genomic Data Delineate Patient-Specific Tumor Profiles and Cancer Stem Cell Organization
Authors: Sottoriva Andrea , Spiteri Inmaculada , Shibata Darryl , Curtis Christina , Tavaré Simon
Published: April 2023

PDF file - 253K