bluuebunny/crossref_metadata_2025 huggingface dataset:
Proteomics Investigations of Potential Protein Biomarkers in Sera of Rabbits Infected With Schistosoma japonicum
Authors: Bi Nian-Nian , Zhao Song , Zhang Jian-Feng , Cheng Ying , Zuo Chen-Yang , Yang Gang-Long , Yang Kun
Published: December 2021

Schistosomiasis is a chronic parasitic disease that continues to be a pressing public health problem in many developing countries. The primary pathological damage from the disease is granuloma and fibrosis caused by egg aggregation, and early treatment can effectively prevent the occurrence of liver fibrosis. Therefore, it is very important to identify biomarkers that can be used for early diagnosis of Schistosoma japonicum infection. In this study, a label-free proteomics method was performed to observe the alteration of proteins before infection, 1 and 6 weeks after infection, and 5 and 7 weeks after treatment. A total of 10 proteins derived from S. japonicum and 242 host-derived proteins were identified and quantified as significantly changed. Temporal analysis was carried out to further analyze potential biomarkers with coherent changes during infection and treatment. The results revealed biological process changes in serum proteins compared to infection and treatment groups, which implicated receptor-mediated endocytosis, inflammatory response, and acute-phase response such as mannan-binding lectin serine peptidase 1, immunoglobulin, and collagen. These findings offer guidance for the in-depth analysis of potential biomarkers of schistosomiasis, host protein, and early diagnosis of S. japonicum and its pathogenesis. Data are available via ProteomeXchange with identifier PXD029635.
Gut Microbiota and Serum Metabolic Signatures of High-Fat-Induced Bone Loss in Mice
Authors: Lu Lingyun , Tang Mengjia , Li Jiao , Xie Ying , Li Yujue , Xie Jinwei , Zhou Li , Liu Yi , Yu Xijie
Published: December 2021

Background

Accumulating evidence indicates that high-fat diet (HFD) is a controllable risk factor for osteoporosis, but the underlying mechanism remains to be elucidated. As a primary biological barrier for nutrient entry into the human body, the composition and function of gut microbiota (GM) can be altered rapidly by HFD, which may trigger abnormal bone metabolism. In the current study, we analyzed the signatures of GM and serum metabolomics in HFD-induced bone loss and explored the potential correlations of GM and serum metabolites on HFD-related bone loss.

Methods

We conducted a mouse model with HFD-induced bone loss through a 12-week diet intervention. Micro-CT, Osmium-μCT, and histological analyses were used to observe bone microstructure and bone marrow adipose tissue. Quantitative Real-Time PCR was applied to analyze gene expression related to osteogenesis, adipogenesis, and osteoclastogenesis. Enzyme-linked immunosorbent assay was used to measure the biochemical markers of bone turnover. 16s rDNA sequencing was employed to analyze the abundance of GM, and UHPLC-MS/MS was used to identify serum metabolites. Correlation analysis was performed to explore the relationships among bone phenotypes, GM, and the metabolome.

Results

HFD induced bone loss accompanied by bone marrow adipose tissue expansion and bone formation inhibition. In the HFD group, the relative abundance of Firmicutes was increased significantly, while Bacteroidetes, Actinobacteria, Epsilonbacteraeota, and Patescibacteria were decreased compared with the ND group. Association analysis showed that thirty-two bacterial genera were significantly related to bone volume per tissue volume (BV/TV). One hundred and forty-five serum metabolites were identified as differential metabolites associated with HFD intervention, which were significantly enriched in five pathways, such as purine metabolism, regulation of lipolysis in adipocyte and cGMP-PKG signaling pathway. Sixty-four diffiential metabolites were matched to the MS2 spectra; and ten of them were positively correlated with BV/TV and five were negatively correlated with BV/TV.

Conclusions

These findings indicated that the alternations of GM and serum metabolites were related to HFD-induced bone loss, which might provide new insights into explain the occurrence and development of HFD-related osteoporosis. The regulatory effects of GM and metabolites associated with HFD on bone homeostasis required further exploration.
Inhibition of SOS Response by Nitric Oxide Donors in Escherichia coli Blocks Toxin Production and Hypermutation
Authors: Crane John K. , Burke Sarah R. , Alvarado Cassandra L.
Published: December 2021

Background

Previous reports have differed as to whether nitric oxide inhibits or stimulates the SOS response, a bacterial stress response that is often triggered by DNA damage. The SOS response is an important regulator of production of Shiga toxins (Stx) in Shiga-toxigenic E. coli (STEC). In addition, the SOS response is accompanied by hypermutation, which can lead to de novo emergence of antibiotic resistance. We studied these effects in vitro as well as in vivo.

Results

Nitric oxide donors inhibited induction of the SOS response by classical inducers such as mitomycin C, ciprofloxacin, and zidovudine, as measured by assays for E. coli RecA. Nitric oxide donors also inhibited Stx toxin protein production as well as stx2 RNA in vitro and in vivo. In vivo experiments were performed with ligated ileal segments in the rabbit using a 20 h infection. The NO donor S-nitroso-acetylpenicillamine (SNAP) reduced hypermutation in vitro and in vivo, as measured by emergence of rifampin resistance. SNAP blocked the ability of the RecA protein to bind to single-stranded DNA in an electrophoretic mobility shift assay (EMSA) in vitro, an early event in the SOS response. The inhibitory effects of SNAP were additive with those of zinc acetate.

Conclusions

Nitric oxide donors blocked the initiation step of the SOS response. Downstream effects of this blockade included inhibition of Stx production and of hypermutation. Infection of rabbit loops with STEC resulted in a downregulation, rather than stimulation, of nitric oxide host defenses at 20 h of infection.
The Water-Energy-Food-Ecosystem Nexus in the Mediterranean: Current Issues and Future Challenges
Authors: De Roo Ad , Trichakis Ioannis , Bisselink Berny , Gelati Emiliano , Pistocchi Alberto , Gawlik Bernd
Published: December 2021

The Mediterranean is an area where the balance between water demand and abstractions vs. water availability is often under stress already, as demonstrated here with the Water Exploitation Index. In this work, model estimates on how different proposed measures for water resources management would affect different indicators. After a review of the current water resources status in the Mediterranean and the definition of indicators used in this study, aspects interlinked with water in the Water-Energy-Food-Ecosystems Nexus are briefly discussed, focusing on problems linked with water scarcity and depletion of groundwater resources as well as with climate change projections. Subsequently, the proposed measures for water efficiency are detailed—irrigation efficiency, urban water efficiency, water reuse and desalination—that might be effective to reduce the growing water scarcity problems in the Mediterranean. Their effects that result from the LISFLOOD model, show that wastewater reuse, desalination and water supply leakage reduction lead to decreased abstractions, but do not affect net water consumption. Increased irrigation efficiency does decrease consumption and reduces abstractions as well. We deduct however that the current envisaged water efficiency measures might not be sufficient to keep up with the pace of diminishing water availability due to climate change. More ambition is needed on water efficiency in the Mediterranean to keep water scarcity at bay.
Language and the Sensing Body: How Sensoriality Permeates Syntax in Interaction
Authors: Mondada Lorenza
Published: December 2021

This article explores the grammar-body interface by examining the intertwinement of embodied practices and turns at talk, wherethe sensing body permeates the ongoing syntax, in particular in activities in which the participants are engaged in talking about sensorial features while at the same time experiencing them, for instance in tasting sessions. So, the question tackled concerns how situated feelings, sensory experiences, and perceptive actions are embedded in the ongoing talk, and how they shape its emergent syntax, possibly affecting its smooth progressivity. The study shows how the choice of specific syntactic formats can be systematically related to the complex ecology of embodied actions, namely to publicly accountable ways of sensing material objects, to ways of showing and addressing an audience, and to visible ways of referring to standard documents normatively defining tasting descriptors. The syntactic formats described and their specific temporal realizations are thus deeply rooted in the local material ecology, in which they not only reproduce a normative model but reflexively express the senses with words and sensuously feel the words.
Serum Free Fatty Acids Independently Predict Adverse Outcomes in Acute Heart Failure Patients
Authors: Yu Yi , Jin Chunna , Zhao Chengchen , Zhu Shiyu , Meng Simin , Ma Hong , Wang Jian'an , Xiang Meixiang
Published: December 2021

Background: Perturbation of energy metabolism exacerbates cardiac dysfunction, serving as a potential therapeutic target in congestive heart failure. Although circulating free fatty acids (FFAs) are linked to insulin resistance and risk of coronary heart disease, it still remains unclear whether circulating FFAs are associated with the prognosis of patients with acute heart failure (AHF).

Methods: This single-center, observational cohort study enrolled 183 AHF patients (de novo heart failure or decompensated chronic heart failure) in the Second Affiliated Hospital, Zhejiang University School of Medicine. All-cause mortality and heart failure (HF) rehospitalization within 1 year after discharge were investigated. Serum FFAs were modeled as quartiles as well as a continuous variable (per SD of FFAs). The restricted cubic splines and cox proportional hazards models were applied to evaluate the association between the serum FFAs level and all-cause mortality or HF rehospitalization.

Results: During a 1-year follow-up, a total of 71 (38.8%) patients had all-cause mortality or HF rehospitalization. The levels of serum FFAs positively contributed to the risk of death or HF rehospitalization, which was not associated with the status of insulin resistance. When modeled with restricted cubic splines, the serum FFAs increased linearly for the incidence of death or HF rehospitalization. In a multivariable analysis adjusting for sex, age, body-mass index, coronary artery disease, diabetes mellitus, hypertension, left ventricular ejection fraction and N-terminal pro-brain natriuretic peptid, each SD (303.07 μmol/L) higher FFAs were associated with 26% higher risk of death or HF rehospitalization (95% confidence interval, 2–55%). Each increasing quartile of FFAs was associated with differentially elevated hazard ratios for death or HF rehospitalization of 1 (reference), 1.71 (95% confidence interval, [0.81, 3.62]), 1.41 (95% confidence interval, [0.64, 3.09]), and 3.18 (95% confidence interval, [1.53, 6.63]), respectively.

Conclusion: Serum FFA levels at admission among patients with AHF were associated with an increased risk of adverse outcomes. Additional studies are needed to determine the causal-effect relationship between FFAs and acute cardiac dysfunction and whether FFAs could be a potential target for AHF management.
Cardiac Magnetic Resonance Radiomics Reveal Differential Impact of Sex, Age, and Vascular Risk Factors on Cardiac Structure and Myocardial Tissue
Authors: Raisi-Estabragh Zahra , Jaggi Akshay , Gkontra Polyxeni , McCracken Celeste , Aung Nay , Munroe Patricia B. , Neubauer Stefan , Harvey Nicholas C. , Lekadir Karim , Petersen Steffen E.
Published: December 2021

Background: Cardiovascular magnetic resonance (CMR) radiomics analysis provides multiple quantifiers of ventricular shape and myocardial texture, which may be used for detailed cardiovascular phenotyping.

Objectives: We studied variation in CMR radiomics phenotypes by age and sex in healthy UK Biobank participants. Then, we examined independent associations of classical vascular risk factors (VRFs: smoking, diabetes, hypertension, high cholesterol) with CMR radiomics features, considering potential sex and age differential relationships.

Design: Image acquisition was with 1.5 Tesla scanners (MAGNETOM Aera, Siemens). Three regions of interest were segmented from short axis stack images using an automated pipeline: right ventricle, left ventricle, myocardium. We extracted 237 radiomics features from each study using Pyradiomics. In a healthy subset of participants (n = 14,902) without cardiovascular disease or VRFs, we estimated independent associations of age and sex with each radiomics feature using linear regression models adjusted for body size. We then created a sample comprising individuals with at least one VRF matched to an equal number of healthy participants (n = 27,400). We linearly modelled each radiomics feature against age, sex, body size, and all the VRFs. Bonferroni adjustment for multiple testing was applied to all p-values. To aid interpretation, we organised the results into six feature clusters.

Results: Amongst the healthy subset, men had larger ventricles with dimmer and less texturally complex myocardium than women. Increasing age was associated with smaller ventricles and greater variation in myocardial intensities. Broadly, all the VRFs were associated with dimmer, less varied signal intensities, greater uniformity of local intensity levels, and greater relative presence of low signal intensity areas within the myocardium. Diabetes and high cholesterol were also associated with smaller ventricular size, this association was of greater magnitude in men than women. The pattern of alteration of radiomics features with the VRFs was broadly consistent in men and women. However, the associations between intensity based radiomics features with both diabetes and hypertension were more prominent in women than men.

Conclusions: We demonstrate novel independent associations of sex, age, and major VRFs with CMR radiomics phenotypes. Further studies into the nature and clinical significance of these phenotypes are needed.
Evolving Threshold of Flood-Leading Precipitation in a User-Oriented Forecast System Based on the TIGGE Dataset
Authors: Zheng Ziyan , Yan Zhongwei , Chen Jing , Han Jiarui , Xia Jiangjiang , Zhang Xiaohong , Cai Siyu , Liu Xiaolin
Published: December 2021

Specific users play a key role in interactive forecast systems through user-oriented information (UOI). For hydrological users, a key component of the user-oriented forecast system (UOFS) is to determine the threshold of flood-leading precipitation (TFLP) as a target of the forecast by considering the decision-making information at the user end. This study demonstrates a novel way of simulating TFLP via the inverse simulation of a hydrological model, combined with the flood hazard assessment in the upper reaches of the Huai River Basin controlled by the Wang Jiaba (WJB) hydrological station. The flood hazard, defined as the probability of precipitation beyond the daily evolving TFLP for the next day, was evaluated by using the THORPEX Interactive Global Grand Ensemble (TIGGE) datasets, including 162 members retrieved from 5 TIGGE archive centers. Having integrated the real-time monitored water level (as the UOI) into the UOFS, we applied it to the flood season of 2008 as a case study to evaluate the flood hazard generated by the UOFS for the WJB sub-basin. The simulated TFLP corresponded well with the gap between the monitored and warning water level. The predicted flood hazard probability showed good agreement with the first two flood peaks at 100% accuracy, while exceeding 60% accuracy for the third flood event in that season. Thus, the flood hazard could be better quantified via integration of the forecasted flood-leading precipitation. Overall, this study highlights the usefulness of a UOFS coupled with interactive UOI of real-time water level to determine the dynamical TFLP for flood hazard evaluation with ensemble precipitation forecast. The early flood warning which resulted from such integrated UOFS is directly applicable to operational flood prevention and mitigation.
Response to the Variation of Clay Minerals During ASP Flooding in the Saertu Oilfield in the Songliao Basin
Authors: Yingjie Liang , Wenfu Liang , Wang He , Zian Li
Published: December 2021

In this paper, the variation of clay minerals and their influence on reservoir physical properties and residual oil before and after ASP flooding are analyzed. The results show that the total amount of clay minerals in reservoirs decreases after ASP flooding in the ultra-high-water-cut-stage reservoirs of the Naner Zone in the Saertu Oilfield, Songliao Basin. Therein, the illite content reduces, while the content of illite smectite mixed-layer and chlorite increases. The content of kaolinite varies greatly. The content of kaolinite decreases in some samples, while it increases in other samples. The clay minerals block the pore throat after ASP flooding. As a result, the pore structure coefficient and the seepage tortuosity increase, the primary intergranular pore throat shrinks, and the pore–throat coordination number decreases. Nevertheless, the dissolution of clay minerals reduces the pore–throat ratio and increases porosity and permeability. The variation of clay minerals after ASP flooding not only intensifies the reservoir heterogeneity but also affects the formation and distribution of residual oil. The residual oil of the oil–clay mixed adsorption state is a newly formed residual oil type related to clay, which accounts for 44.2% of the total residual oil reserves, so it is the main occurrence form of the oil in reservoirs after ASP flooding. Therefore, the exploitation of this type of residual oil has great significance to enhance the oil recovery in ultra-high-water-cut-stage reservoirs.
A Study to Investigate the Viscosity Effect on Micro-Confined Fluids Flow in Tight Formations Considering Fluid–Solid Interaction
Authors: Chen Mingqiang , Li Qingping , Cheng Linsong , Wang Xiukun , Lyu Chaohui , Fan Qi
Published: December 2021

Understanding different fluids flow behavior confined in microscales has tremendous significance in the development of tight oil reservoirs. In this article, a novel semiempirical model for different confined fluid flow based on the concept of boundary layer thickness, caused by the fluid–solid interaction, is proposed. Micro-tube experiments are carried out to verify the novel model. After the validation, the viscosity effect on the flow rate and Poiseuille number considering the fluid–solid interaction is investigated. Furthermore, the novel model is incorporated into unstructured networks with anisotropy to study the viscosity effect on pore-scale flow in tight formations under the conditions of different displacement pressure gradients, different aspect ratios (ratio of the pore radius to the connecting throat radius), and different coordination numbers. Results show that the viscosity effect on the flow rate and Poiseuille number after considering the fluid–solid interaction induces a great deviation from that in conventional fluid flow. The absolute permeability is not only a parameter related to pore structures but also depends on fluid viscosity. The study provides an effective model for modeling different confined fluid flow in microscales and lays a good foundation for studying fluid flow in tight formations.
Mechanical Properties of Frozen Glacial Tills due to Short Periods of Thawing
Authors: Fu Yanju , Jiang Yao , Wang Jiao , Liu Ziming , Lu Xingsheng
Published: December 2021

Due to the warming climate, glacier retreat has left massive glacial tills in steep gullies; ice in the soil is prone to change phase resulting in the decrease of the ice strength and bonding of soil particles; collapse of thawing tills can lead to debris flows with disastrous consequences for geotechnical infrastructures. To improve our understanding of the mechanics of thawing glacial tills, we conducted unconsolidated–undrained direct shear tests on glacial tills from Tianmo gully on the southeastern Tibetan Plateau. Control specimens were not subjected to freeze–thaw action. A total of 648 specimens with three different dry densities, three initial water contents, and 18 thawing times were tested. Peak shear strength, peak stress to displacement ratio (0.857), and cohesion were the highest in frozen specimens. After a thawing time of 0.25 h, there was a marked decline in shear strength; maximum friction was 2.58, which was far below the value of cohesive strength. For thawing times of 0.25–4 h, peak strength varied little with thawing time, but cohesion decreased and internal friction angle increased with increasing thawing time. Our results indicate that thawing of the solid ice in the till during the initial phase of till thawing is the key control of peak till strength; the effect of ice on cohesion is greater during the initial phase of thawing and in loose tills. Moreover, frequent sediment recharge of gullies may be explained by the decrease of cohesion with increasing thawing time caused by short-term destruction of ice bonding.
Size Effect on Mechanical Behaviors of Intact Rocks Based on Enlarged Digital Models
Authors: Xiao Huaiguang , He Lei , Shi Run , Song Jiacheng , Tang Yudi , Zhang Qi , Shi Peng
Published: December 2021

The size effect on mechanical behaviors of rock materials has been a challenging topic. The previous research is mainly to study the relationship between known large-scale models and representative element volumes. However, only limited scale models can be obtained in situ or laboratory in many cases, so tests or simulations of mechanical response behaviors of large-scale models cannot be carried out. In order to obtain large-scale models, this paper proposes a multi-scale enlarged digital modeling method to synthesize granite models based on texture self-similarity. The representational ability of synthetic granite is analyzed by using mathematical statistics and finite-difference numerical method. The results show that synthetic granite can characterize the natural rock well in terms of texture morphology, geometric characteristics, and mechanical behaviors. Then, the size effect on mechanical behaviors of intact granite is performed based on an enlarged digital model. The results show that the uniaxial compressive strength of intact granite increases with the increase of model size. In addition, the relationship between heterogeneity and homogeneity is established at different unit scales by using the synthetic digital granite model. The enlarged digital rock model has great potential in studying size effects with rock heterogeneity.
Growth in Children With Noonan Syndrome and Effects of Growth Hormone Treatment on Adult Height
Authors: Libraro Annachiara , D’Ascanio Vito , Cappa Marco , Chiarito Mariangela , Digilio Maria Cristina , Einaudi Silvia , Grandone Anna , Maghnie Mohamad , Mazzanti Laura , Mussa Alessandro , Patti Giuseppa , Scarano Emanuela , Spinuzza Antonietta , Vannelli Silvia , Wasniewska Malgorzata Gabriela , Ferrero Giovanni Battista , Faienza Maria Felicia
Published: December 2021

Objectives

Growth impairment is a common manifestation in Noonan syndrome (NS). Recombinant human GH (rhGH) treatment has been shown to increase growth and adult height (AH) in a few studies. We aimed to evaluate the growth trajectory towards the AH, and the effects of rhGH treatment in a large cohort of NS children.

Methods

Retrospective, multicenter, cohort study including subjects with genetic diagnosis of NS. A total of 228 NS patients, 154 with PTPN11 mutations, 94 who reached AH, were recruited. Auxological data were collected at 2, 5, and 10 years, at pubertal onset, at AH. Sixty-eight NS subjects affected with GH deficiency (GHD) were treated with rhGH at a mean dose of 0.24 mg/kg per week until AH achievement.

Results

ANOVA analysis showed a significant difference between birth length and height standard deviation scores (HSDS) at the different key ages (p<0.001), while no significant differences were found between HSDS measurements at 2, 5, and 10 years, at pubertal onset, and at AH. HSDS increased from −3.10 ± 0.84 to −2.31 ± 0.99 during rhGH treatment, with a total height gain of 0.79 ± 0.74, and no significant difference between untreated and treated NS at AH.

Conclusions

rhGH treatment at the standard dose used for children with GH idiopathic deficiency is effective in improving growth and AH in NS with GHD. Further studies are needed to assess genotype-specific response to rhGH treatment in the different pathogenic variants of PTPN11 gene and in the less common genotypes.
Head-to-Head Comparison of 68Ga-PSMA-11 and 131I in the Follow-Up of Well-Differentiated Metastatic Thyroid Cancer: A New Potential Theragnostic Agent
Authors: Pitalua-Cortes Quetzali , García-Perez Francisco Osvaldo , Vargas-Ahumada Joel , Gonzalez-Rueda Sofia , Gomez-Argumosa Edgar , Ignacio-Alvarez Eleazar , Soldevilla-Gallardo Irma , Torres-Agredo Liliana
Published: December 2021

Introduction

Thyroid cancer is the main endocrine neoplasia worldwide, for which 131I therapy is the cornerstone treatment. One of the main problems of follow up in patients with this type of cancer, is the need for thyroglobulin stimulation, not to mention the poor availability of 123I or 124I, to perform studies with a higher degree of sensitivity. Prostatic Specific Membrane Antigen (PSMA) PET/CT has demonstrated to be quite useful in a diversified number of neoplasms, on behalf of its capacity of evaluating the extent of type II carboxypeptidase expression in vascular endothelium. The end point of this article is to assess whether this novel image method possesses applicability in thyroid neoplasms follow up, for diagnostic and potentially therapeutic purposes.

Methods

We retrospectively evaluated well differentiated metastatic thyroid cancer patients, who underwent a post therapeutic 131I dose whole body scan (WBS) and complementary SPECT/CT, as well as 68Ga-PSMA–11 PET/CT.

Results

Ten patients with differentiated thyroid cancer were included, of whom 80% were women and 20% men, mean age was 58 years old (± 11.6). Sixty-four metastatic lesions were analyzed, 67.19% had papillary histology and 32.81% were follicular type, the most affected site of metastases was bone in 57.81%, followed by lung 17.19%, lymph nodes 7.81%, postoperative thyroid bed 4.69%, brain 4.69% and others 7.81%. 68Ga PSMA-11 PET/CT detected 64/64 lesions, all of them also identified by computed tomography (CT), whereas 131I SPECT/CT detected 55/64 lesions. Discrepant lesions were localized in lung 44.4%, brain 22.2%, postoperative thyroid bed 11.1%, lymph nodes 11.1% and bone 11.1%. The degree of correspondence among observers was outstanding for both radiotracers, but close upon perfect for PSMA-11 (κ = 0.98; 95% CI, 0.80 – 0.91), as opposed to 131 I (κ = 0.86; 95% CI, 0.71 – 0.76).

Conclusions

68Ga-PSMA PET/CT showed an utterly superior capability for metastatic lesion detection when compared to 131I SPECT/CT. These findings suggest that PSMA PET/CT could possibly and precociously identify radioiodine refractoriness. PSMA uptake values not only expedite diagnosis, but also award it the ability to be used for therapeutic intents.
Endoscopic Lateral Neck Dissection: A New Frontier in Endoscopic Thyroid Surgery
Authors: Zhang Zeyu , Sun Botao , Ouyang Hui , Cong Rong , Xia Fada , Li Xinying
Published: December 2021

Background

Endoscopic thyroidectomy and robotic thyroidectomy are effective and safe surgical options for thyroid surgery, with excellent cosmetic outcomes. However, in regard to lateral neck dissection (LND), much effort is required to alleviate cervical disfigurement derived from a long incision. Technologic innovations have allowed for endoscopic LND, without the need for extended cervical incisions and providing access to remote sites, including axillary, chest–breast, face-lift, transoral, and hybrid approaches.

Methods

A comprehensive review of published literature was performed using the search terms “lateral neck dissection”, “thyroid”, and “endoscopy OR endoscopic OR endoscope OR robotic” in PubMed.

Results

This review provides an overview of the current knowledge regarding endoscopic LND, and it specifically addresses the following points: 1) the surgical procedure, 2) the indications and contraindications, 3) the complications and surgical outcomes, and 4) the technical advantages and limitations. Robotic LND, totally endoscopic LND, and endoscope-assisted LND are separately discussed.

Conclusions

Endoscopic LND is a feasible and safe technique in terms of complete resection of the selected neck levels, complications, and cosmetic outcomes. However, it is recommended to strictly select criteria when expanding the population of eligible patients. A formal indication for endoscopic LND has not yet been established. Thus, a well-designed, multicenter study with a large cohort is necessary to confirm the feasibility, long-term outcomes, oncological safety, and influence of endoscopic LND on patient quality of life (QoL).
Neutronics Perturbation Calculation Method Study of Solid Breeder Tritium Breeding Blanket for TBR Enhancement
Authors: Qu Shen , Cao Qixiang , Zhao Fengchao , Wang Xueren , Duan Xuru , Wang Xiaoyu
Published: December 2021

Tritium breeding blanket (TBB) is an essential component in a fusion reactor, which has functions of tritium breeding, energy generation, and neutron shielding. Tritium breeding ratio (TBR) is a key parameter to evaluate whether the TBB could produce enough tritium to achieve the tritium self-sufficiency (TBR >1) for fusion reactor. Current codes or software are hard to meet the requirements of high efficiency, high resolution, and high automation for neutronic optimization of TBB. In this article, the application of the density perturbation calculation on a solid breeder TBB was first performed. Then, the method of the geometry perturbation calculation based on the virtual density theory was studied. Results and comparison analysis indicate that the 1st + 2nd-order neutronic perturbation calculations (including the density perturbation and the geometry perturbation) results are consistent with the transport results under a perturbation of −15% to +15%. It is proven to be valid to use the perturbation calculation for rapid TBR enhancement study of the solid breeder TBB.
Impact of Social Capital on Environmental Governance Efficiency—Behavior of Guangdong, China
Authors: Zhang Shujian , Gu Zhijun
Published: December 2021

The main purpose of this article is to investigate the impact of social capital level on environmental governance efficiency in economically developed areas. We choose China’s richest province Guangdong. Compared with other Chinese provinces, Guangdong’s local governments are in better fiscal status, and they can allocate environmental spending in line with the trend of gross domestic product per capita. We want to observe the important factors related to social cognition other than capital investment to gain a more profound influence on the efficiency of local environmental governance. This article assumes that the degree of local social capital will have a great positive impact on the efficiency of regional environmental governance. Super-efficiency slacks-based measure method of data envelopment analysis is adopted to calculate the environmental governance efficiency by considering the expected output and the nonexpected output. Then, short-panel regression was used to analyze the relationship between social capital and environmental governance efficiency. Through an empirical analysis of urban panel data of Guangdong province from 2001 to 2019, it is found that the degree of social capital does have a significant positive impact on the efficiency of local environmental governance. After the research conclusion, we propose some policy suggestions to local governments.
P2H Modeling and Operation in the Microgrid Under Coupled Electricity–Hydrogen Markets
Authors: Liu Hongxin , Wang Yueyao , Xu Feifei , Wu Mengkai , Jiang Kai , Yan Xiaohe , Liu Nian
Published: December 2021

The uncertainty and volatility of wind power have led to large-scale wind curtailment during grid connections. The adoption of power-to-hydrogen (P2H) system in a microgrid (MG) can mitigate the renewable curtailment by hydrogen conversion and storage. This paper conducts unified modeling for different types of P2H systems and considers the multi-energy trading in a hydrogen-coupled power market. The proposed bi-level equilibrium model is beneficial to minimize the energy cost of microgrids. Firstly, a microgrid operation model applied to different P2H systems including an alkaline electrolysis cell (AEC), a proton exchange membrane electrolysis cell (PEMEC), or a solid oxide electrolysis cell (SOEC) is proposed at the upper level. Secondly, an electricity market–clearing model and a hydrogen market model are constructed at the lower level. Then, the diagonalization algorithm is adopted to solve the multi-market equilibrium problem. Finally, case studies based on an IEEE 14-bus system are conducted to validate the proposed model, and the results show that the microgrid with a P2H system could gain more profits and help increase the renewable penetration.
Long Distance Seed Dispersal by Forest Elephants
Authors: Poulsen John R. , Beirne Christopher , Rundel Colin , Baldino Melissa , Kim Seokmin , Knorr Julia , Minich Taylor , Jin Lingrong , Núñez Chase L. , Xiao Shuyun , Mbamy Walter , Obiang Guichard Ndzeng , Masseloux Juliana , Nkoghe Tanguy , Ebanega Médard Obiang , Clark Connie J. , Fay Michael J. , Morkel Pete , Okouyi Joseph , White Lee J. T. , Wright Justin P.
Published: December 2021

By dispersing seeds long distances, large, fruit-eating animals influence plant population spread and community dynamics. After fruit consumption, animal gut passage time and movement determine seed dispersal patterns and distances. These, in turn, are influenced by extrinsic, environmental variables and intrinsic, individual-level variables. We simulated seed dispersal by forest elephants (Loxodonta cyclotis) by integrating gut passage data from wild elephants with movement data from 96 individuals. On average, elephants dispersed seeds 5.3 km, with 89% of seeds dispersed farther than 1 km. The longest simulated seed dispersal distance was 101 km, with an average maximum dispersal distance of 40.1 km. Seed dispersal distances varied among national parks, perhaps due to unmeasured environmental differences such as habitat heterogeneity and configuration, but not with human disturbance or habitat openness. On average, male elephants dispersed seeds farther than females. Elephant behavioral traits strongly influenced dispersal distances, with bold, exploratory elephants dispersing seeds 1.1 km farther than shy, idler elephants. Protection of forest elephants, particularly males and highly mobile, exploratory individuals, is critical to maintaining long distance seed dispersal services that shape plant communities and tropical forest habitat.
Differential lncRNA/mRNA Expression Profiling and Functional Network Analyses in Bmp2 Deletion of Mouse Dental Papilla Cells
Authors: Wang Feng , Tao Ran , Zhao Li , Hao Xin-Hui , Zou Yi , Lin Qing , Liu Meng Meng , Goldman Graham , Luo Daoshu , Chen Shuo
Published: December 2021

Bmp2 is essential for dentin development and formation. Bmp2 conditional knock-out (KO) mice display a similar tooth phenotype of dentinogenesis imperfecta (DGI). To elucidate a foundation for subsequent functional studies of cross talk between mRNAs and lncRNAs in Bmp2-mediated dentinogenesis, we investigated the profiling of lncRNAs and mRNAs using immortalized mouse dental Bmp2 flox/flox (iBmp2fx/fx) and Bmp2 knock-out (iBmp2ko/ko) papilla cells. RNA sequencing was implemented to study the expression of the lncRNAs and mRNAs. Quantitative real-time PCR (RT-qPCR) was used to validate expressions of lncRNAs and mRNAs. The Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases were used to predict functions of differentially expressed genes (DEGs). Protein–protein interaction (PPI) and lncRNA–mRNA co-expression network were analyzed by using bioinformatics methods. As a result, a total of 22 differentially expressed lncRNAs (16 downregulated vs 6 upregulated) and 227 differentially expressed mRNAs (133 downregulated vs. 94 upregulated) were identified in the iBmp2ko/ko cells compared with those of the iBmp2fx/fx cells. RT-qPCR results showed significantly differential expressions of several lncRNAs and mRNAs which were consistent with the RNA-seq data. GO and KEGG analyses showed differentially expressed genes were closely related to cell differentiation, transcriptional regulation, and developmentally relevant signaling pathways. Moreover, network-based bioinformatics analysis depicted the co-expression network between lncRNAs and mRNAs regulated by Bmp2 in mouse dental papilla cells and symmetrically analyzed the effect of Bmp2 during dentinogenesis via coding and non-coding RNA signaling.
miR-214-5p Regulating Differentiation of Intramuscular Preadipocytes in Goats via Targeting KLF12
Authors: Du Yu , Wang Yong , Li Yanyan , Emu Quzhe , Zhu Jiangjiang , Lin Yaqiu
Published: December 2021

Intramuscular fat (i.m.) is an adipose tissue that is deposited between muscle bundles. An important type of post-transcriptional regulatory factor, miRNAs, has been observed as an important regulator that can regulate gene expression and cell differentiation through specific binding with target genes, which is the pivotal way determining intramuscular fat deposition. Thus, this study intends to use RT-PCR, cell culture, liposome transfection, real-time fluorescent quantitative PCR (qPCR), dual luciferase reporter systems, and other biological methods clarifying the possible mechanisms on goat intramuscular preadipocyte differentiation that is regulated by miR-214-5p. Ultimately, our results showed that the expression level of miR-214-5p peaked at 48 h after the goat intramuscular preadipocytes were induced for adipogenesis. Furthermore, after inhibition of the expression of miR-214-5p, the accumulation of lipid droplets and adipocyte differentiation in goat intramuscular adipocytes were promoted by the way of up-regulation of the expression level of lipoprotein lipase (LPL) (p < 0.05) and peroxisome proliferator-activated receptor gamma (PPARγ) (p < 0.01) but inhibited the expression of hormone-sensitive lipase (HSL) (p < 0.01). Subsequently, our study confirmed that Krüppel-like factor 12 (KLF12) was the target gene of miR-214-5p. Inhibition of the expression of KLF12 promoted adipocyte differentiation and lipid accumulation by upregulation of the expression of LPL and CCAAT/enhancer binding protein (C/EBPα) (p < 0.01). Overall, these results indicated that miR-214-5p and its target gene KLF12 were negative regulators in progression of goat preadipocyte differentiation. Our research results provided an experimental basis for finally revealing the mechanism of miR-214-5p in adipocytes.
A Haptoglobin Exon Copy Number Variant Associates With HIV-Associated Neurocognitive Impairment in European and African-Descent Populations
Authors: Bai Haimeng , Kaur Harpreet , Kallianpur Asha R. , Hulgan Todd , Franklin Donald R. , Letendre Scott L. , Ellis Ronald J. , Bush William S.
Published: December 2021

A common two-exon deletion distinguishes the gene encoding the free hemoglobin capturing protein—haptoglobin (HP)–into two alleles: HP1 and HP2. To evaluate the impact of this copy number variant (CNV) on neurocognitive impairment (NCI) in people living with HIV, we imputed this variant in 432 European-descent (EUR) and 491 African-descent (AFR) participants from the CNS HIV Antiretroviral Therapy Effects Research Study using an optimized imputation pipeline and evaluated its associations with NCI. At baseline, in AFR, the HP2 allele decreased the odds of NCI (defined by a global deficit score, GDS, ⩾0.5; Odds Ratio, OR = 0.584, p = 0.022). However, in EUR, HP2 increased the odds (OR = 2.081, p = 0.040) of NCI suggesting a detrimental effect. These effects were extended to longitudinal analyses using repeated measurements where the protective effect of the HP2 allele in AFR became marginally significant (p = 0.054) and in EUR the detrimental effect increased in significance (p = 0.037). In EUR, the HP2 allele slightly reduced the risk of NCI over time (OR = 0.028 per allele per year, p = 0.024). Further analyses of cognitive domain-specific impairment revealed that the HP-NCI effect was based on changes in learning, speed of information processing, and verbal domains over time differing by ancestry groups. Overall, these findings suggest that these functional HP CNV alleles influence the likelihood of NCI and contribute to changes in neurocognitive function over time in people living with HIV.
Somatic piRNAs and Transposons are Differentially Expressed Coincident with Skeletal Muscle Atrophy and Programmed Cell Death
Authors: Tsuji Junko , Thomson Travis , Brown Christine , Ghosh Subhanita , Theurkauf William E. , Weng Zhiping , Schwartz Lawrence M.
Published: December 2021

PIWI-interacting RNAs (piRNAs) are small single-stranded RNAs that can repress transposon expression via epigenetic silencing and transcript degradation. They have been identified predominantly in the ovary and testis, where they serve essential roles in transposon silencing in order to protect the integrity of the genome in the germline. The potential expression of piRNAs in somatic cells has been controversial. In the present study we demonstrate the expression of piRNAs derived from both genic and transposon RNAs in the intersegmental muscles (ISMs) from the tobacco hawkmoth Manduca sexta. These piRNAs are abundantly expressed, ∼27 nt long, map antisense to transposons, are oxidation resistant, exhibit a 5’ uridine bias, and amplify via the canonical ping-pong pathway. An RNA-seq analysis demonstrated that 19 piRNA pathway genes are expressed in the ISMs and are developmentally regulated. The abundance of piRNAs does not change when the muscles initiate developmentally-regulated atrophy, but are repressed coincident with the commitment of the muscles undergo programmed cell death at the end of metamorphosis. This change in piRNA expression is correlated with the repression of several retrotransposons and the induction of specific DNA transposons. The developmentally-regulated changes in the expression of piRNAs, piRNA pathway genes, and transposons are all regulated by 20-hydroxyecdysone, the steroid hormone that controls the timing of ISM death. Taken together, these data provide compelling evidence for the existence of piRNA in somatic tissues and suggest that they may play roles in developmental processes such as programmed cell death.
Case Report: First Case of Cefotaxime-Sulbactam-Induced Acute Intravascular Hemolysis in a Newborn With ABO Blood Type Incompatibility by the Mechanism of Non-Immunologic Protein Adsorption
Authors: Wu Yuanjun , Wu Yong , Yang Yong , Chen Baochan , Li Jianqun , Guo Ganping , Xiong Fu
Published: December 2021

Background

ABO blood type incompatibility hemolytic disease of newborn (ABO-HDN) and drug-induced immune hemolytic anemia (DIIHA) due to non-immunologic protein adsorption (NIPA) mainly cause extravascular hemolysis. All the reported severe DIIHA were caused by drug-induced antibodies, and rare report of acute intravascular hemolysis was caused by the NIPA mechanism or ABO-HDN.

Case presentation

We report the first case of acute intravascular hemolysis induced by cefotaxime sodium - sulbactam sodium (CTX - SBT) in a case of ABO-HDN which resulted in death at 55 h after birth. The mother’s blood type was O and RhD-positive, and the newborn’s blood type was B and RhD-positive. No irregular red blood cell (RBC) antibodies or drug-dependent antibodies related to CTX or SBT was detected in the mother’s plasma and the plasma or the RBC acid eluent of the newborn. Before the newborn received CTX - SBT treatment, the result of direct antiglobulin test (DAT) was negative while anti-B was positive (2 +) in both plasma and acid eluent. After the newborn received CTX - SBT treatment, the results of DAT for anti-IgG and anti-C3d were both positive, while anti-B was not detected in plasma, but stronger anti-B (3 +) was detected in acid eluent. In vitro experiments confirmed that NIPA of SBT promoted the specific binding of maternal-derived IgG anti-B to B antigen on RBCs of the newborn, thereby inducing acute intravascular hemolysis.

Conclusion

The NIPA effect of SBT promoted the specific binding of mother-derived IgG anti-B in newborn’s plasma to the newborn’s RBC B antigens and formed an immune complex, and then activated complement, which led to acute intravascular hemolysis. Drugs such as SBT with NIPA effect should not be used for newborns with HDN.
Saliva and Serum Cytokine Profiles During Oral Ulceration in Behçet’s Disease
Authors: Novak Tanya , Hamedi Mojgan , Bergmeier Lesley Ann , Fortune Farida , Hagi-Pavli Eleni
Published: December 2021

Behçet’s disease (BD) is a chronic, multi-systemic disorder of unknown aetiology typified by recurrent oral and genital mucocutaneous lesions, uveitis and vasculitis. Innate and adaptive immune system dysregulation has been implicated in pathogenesis with alterations in serum cytokine profiles. Few studies have investigated salivary cytokines in BD, despite more than 90% of BD patients first presenting with oral ulceration. The aim of this pilot study was twofold; firstly to investigate whether cytokine levels in matched serum and saliva samples show a differential profile in BD (with and without oral ulcers), recurrent aphthous stomatitis (RAS) and healthy controls (HCs), and secondly, to explore if any differential profiles in serum and/or saliva could provide a panel of cytokines with diagnostic and therapeutic potential for BD. Concentrations of 12 cytokines (IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12p70, IL-17A, IFN-γ, TNF-α, TNF-β) were measured using the Human Th1/Th2 11-Plex FlowCytomix™ kit with IL-17A, in BD (N=20), RAS (N=6) and HCs (N=10). A differential range of cytokines was detected in serum and saliva with the majority of cytokine levels higher in saliva. The most prevalent salivary cytokines were IL-1β, IL-2, IL-8, IL-10 and TNF-α present in all samples in contrast to serum where the most prevalent cytokine detected was IL-8 (91.9%). The least abundant cytokine was IFN-γ in both saliva (43.2%) and serum (2.7%). After normalizing saliva for protein content, BD patients with oral ulcers (BD-MA) had significantly higher levels of salivary IL-1β (p=0.01), IL-8 (p=0.02), TNF-α (p=0.004) and IL-6 (p=0.01) than HCs. Notably, BD patients without oral ulcers (BD-MQ) also had significantly higher salivary IL-1β, IL-8 and TNF-α (p ≤ 0.05) than HCs. During relapsed (BD-RE) and quiet (BD-Q) systemic episodes, salivary IL-β and TNF-α were also significantly increased with IL-8 significantly higher only in BD-Q (p=0.02). BD oral ulcers signify a potential reactivation of systemic inflammation. Identifying cytokines released during asymptomatic episodes and oral ulceration might lead to targeted drug therapy to prevent recurrent oral ulcers and possible disease relapse. This is the first study to report salivary cytokine levels in BD. The detectable levels suggests cytokine profiling of BD saliva may provide an alternative, less invasive, sensitive procedure for frequent monitoring of disease activity and progression.