bluuebunny/crossref_metadata_2025 huggingface dataset:
Immunomodulatory Effect of Polysaccharide from Fermented Morinda citrifolia L. (Noni) on RAW 264.7 Macrophage and Balb/c Mice
Authors: Choi Sun-Il , La Im-Joung , Han Xionggao , Men Xiao , Lee Se-Jeong , Oh Geon , Kwon Hee-Yeon , Kim Yong-Deok , Seong Geum-Su , Kim Seung-Hyung , Lee Ok-Hwan
Published: June 2022

This study aims to determine the immunomodulatory effects of a polysaccharide fraction from fermented M. citrifolia L. (FMP) in RAW 264.7 macrophages and Balb/c mice. M. citrifolia was fermented for 72 h using Lactobacillus brevis; polysaccharides were extracted using ethanol precipitation. The RAW 264.7 cells exposed to FMP (50, 100, and 200 μg/mL) for 24 h showed increased NO production, proinflammatory cytokine (IL-1β, IL-6, and TNF-α) release, and COX-2 and iNOS protein expression. FMP (100, 200 mg/kg) and deacetylasperulosidic acid (DAA) (20 mg/kg) administered orally to Balb/c mice for 14 days upregulated NO production and NK cytotoxicity in abdominal cavity and spleen, respectively. Th1 and Th2 cytokines production and immune cell numbers increased in spleen, mesenteric lymph nodes (MLN), peritoneal exudate cells (PEC), Peyer’s patches (PP), and peripheral blood mononuclear cells (PBMC). Therefore, FMP containing DAA can be used as materials for health functional foods to enhance immune responses.
Temperature Effects on Core g-Modes of Neutron Stars
Authors: Lozano Nicholas , Tran Vinh , Jaikumar Prashanth
Published: June 2022

Neutron stars provide a unique physical laboratory in which to study the properties of matter at high density and temperature. We study a diagnostic of the composition of high-density matter, namely, g-mode oscillations, which are driven by buoyancy forces. These oscillations can be excited by tidal forces and couple to gravitational waves. We extend prior results for the g-mode spectrum of cold neutron star matter to high temperatures that are expected to be achieved in neutron star mergers using a parameterization for finite-temperature effects on equations of state recently proposed by Raithel, Özel and Psaltis. We find that the g-modes of canonical mass neutron stars (≈1.4M⊙) are suppressed at high temperatures, and core g-modes are supported only in the most massive (≥2M⊙) of hot neutron stars.
Concentration-Dependent Emission of Annealed Sol-Gel Layers Incorporated with Rhodamine 19 and 6G as the Route to Tunable High-Temperature Luminescent Materials
Authors: Zdończyk Maria , Potaniec Bartłomiej , Fiedot-Toboła Marta , Baraniecki Tomasz , Cybińska Joanna
Published: June 2022

The sol-gel technology allows for the development of materials for nonlinear optics and photonics through the synthesis of multifunctional ceramic materials. Although the nature of the amorphous matrix allows the material to be doped with a large amount of the active components without quenching, it may affect the spectroscopic characteristics of the dye (e.g., result in a shift of absorption and emission peaks with drying time, presumably with a change of concentration). This study presents the material (SiO2 impregnated with organic dyes—Rhodamine 6G and 19) with tunable emissions obtained by the authors upon annealing at different temperatures within the range of 100–300 °C. Possible observed effects were discussed based on spectroscopic properties and thermal studies of the synthesized material. Concerning annealing at different temperatures, an effect on concentration was observed. At the same time, a longer heating process at 300 °C revealed a protective function of sol-gel-derived silica for the organic dye; the longer heating did not cause any further significant changes in the dye’s emission, which indicates the preservative role of the sol-gel layers. Furthermore, etching tests of thin layers were conducted, resulting in smooth side edges of the waveguide. The tests have shown that it is possible to use dye-doped sol-gel layers as active components in photonics platforms.
Significant Interfacial Dielectric Relaxation of Covalently Bonded Ice-Hydrogels
Authors: Li Yongqiang , Chen Liufang , Li Chuanfu , Lin Lin , Yan Zhibo , Liu Junming
Published: June 2022

Hydrogels are composed of a three-dimensional network of cross-linked hydrophilic polymer chains and large amounts of water. The physicochemical properties of the polymer-water interface in hydrogels draw our attention. Due to the complex structure of hydrogel systems, it is still a challenge to investigate the interfacial layer properties of hydrogels through experiments. In this work, we investigate the properties of the covalently bonded chitosan-based ice-hydrogels interfacial layer by dielectric relaxation spectroscopy (DRS) techniques in the presence of avoided electrode polarization. The DRS data exhibit that the polymer-water interfacial layer has a strong dielectric signal response, which indicates that a large number of polar electric dipoles or polar molecules may be contained in the interfacial layer. The variable temperature dielectric relaxation behavior of a series of chitosan-base ice-hydrogels showed that the value of dielectric activation energy for different water contents is about 180 kJ/mol, which is much larger than that of the polymer and ice phases, suggesting a strong coupling of polar electric dipoles within the interfacial layer. This work demonstrates the important role of the polymer-water interface in covalently bonded hydrogels, which will provide assistance in the design and application of covalently bonded hydrogels.
Influence of Gel Stage from Cellulose Dissolution in NaOH-Water System on the Performances of Cellulose Allomorphs-Based Hydrogels
Authors: Ciolacu Diana Elena , Rusu Daniela , Darie-Niţă Raluca Nicoleta , Tîmpu Daniel , Ciolacu Florin
Published: June 2022

Novel hydrogels were prepared starting from different cellulose allomorphs (cellulose I, II, and III), through a swelling stage in 8.5% NaOH aqueous solution, followed by freezing at low temperature (−30 °C), for 24 h. After thawing at room temperature, the obtained gels were chemical cross-linked with epichlorohydrin (ECH), at 85 °C. The swelling degrees of the hydrogels were investigated, and a complex dependence on the type of the cellulose allomorph was found. Moreover, the gel stage has been shown to play a key role in the design of hydrogels with different performances, following the series: H-CII > H-CI > H-CIII. The correlations between the allomorph type and the morphological characteristics of hydrogels were established by scanning electron microscopy (SEM). The hydrogel H-CII showed the biggest homogeneous pores, while H-CIII had the most compacted pores network, with small interconnected pores. The rheological studies were performed in similar shear regimes, and a close correlation between the strength of the gel structure and the size of the gel fragments was observed. In the case of hydrogels, it has been shown that H-CII is softer, with a lower resistance of the hydrogel (G′) above the oscillation frequencies tested, but it maintains its stable structure, while H-CIII has the highest modulus of storage and loss compared to H-CI and H-CII, having a stronger and more rigid structure. The X-ray diffraction (XRD) method showed that the crystalline organization of each type of allomorph possesses a distinctive diffraction pattern, and, in addition, the chemically cross-linking reaction has been proved by a strong decrease of the crystallinity. Attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy provided clear evidence of the chemical cross-linking of cellulose allomorphs with ECH, by the alteration of the crystal structure of cellulose allomorphs and by the formation of new ether bands.
A Systematic Review of Clinical Outcomes from Pharmacist Provided Medication Therapy Management (MTM) among Patients with Diabetes, Hypertension, or Dyslipidemia
Authors: Marupuru Srujitha , Roether Alexis , Guimond A. J. , Stanley Chris , Pesqueira Tyler , Axon David R.
Published: June 2022

This study aimed to compare the clinical outcomes of pharmacist-provided medication therapy management (MTM) services as compared to no MTM services (i.e., standard of care) on outpatient clinical outcomes for patients with diabetes, hypertension, or dyslipidemia. A systematic literature review of PubMed, EMBASE, Cochrane library, International Pharmaceutical Abstracts, PsycINFO, Scopus, CINAHL electronic databases, grey literature, websites, and journals, was conducted from 1 January 2005–20 July 2021. The search field contained a combination of keywords and MeSH terms such as: “medication therapy management”, “pharmacist”, “treatment outcomes”. Studies published in United States, included adults ≥18 years old who received at least one pharmacist-provided MTM consultation and at least one group who received no MTM, and reported pre-specified clinical outcomes for diabetes mellitus, hypertension, or dyslipidemia were included. Of 849 studies identified, eight were included (cohort studies = 6, randomized controlled trials = 2). Clinical outcomes improved with MTM interventions, as evidenced by statistically significant changes in at least one of the three chronic conditions in most studies. Improvements were observed for diabetes outcomes (n = 4 studies), hypertension outcomes (n = 4 studies), and dyslipidemia outcomes (n = 3 studies). Overall, this study indicated that pharmacist delivered MTM services (versus no MTM services) can improve clinical outcomes for patients with diabetes, hypertension, and dyslipidemia.
IMU-Based Monitoring for Assistive Diagnosis and Management of IoHT: A Review
Authors: Bo Fan , Yerebakan Mustafa , Dai Yanning , Wang Weibing , Li Jia , Hu Boyi , Gao Shuo
Published: June 2022

With the rapid development of Internet of Things (IoT) technologies, traditional disease diagnoses carried out in medical institutions can now be performed remotely at home or even ambient environments, yielding the concept of the Internet of Health Things (IoHT). Among the diverse IoHT applications, inertial measurement unit (IMU)-based systems play a significant role in the detection of diseases in many fields, such as neurological, musculoskeletal, and mental. However, traditional numerical interpretation methods have proven to be challenging to provide satisfying detection accuracies owing to the low quality of raw data, especially under strong electromagnetic interference (EMI). To address this issue, in recent years, machine learning (ML)-based techniques have been proposed to smartly map IMU-captured data on disease detection and progress. After a decade of development, the combination of IMUs and ML algorithms for assistive disease diagnosis has become a hot topic, with an increasing number of studies reported yearly. A systematic search was conducted in four databases covering the aforementioned topic for articles published in the past six years. Eighty-one articles were included and discussed concerning two aspects: different ML techniques and application scenarios. This review yielded the conclusion that, with the help of ML technology, IMUs can serve as a crucial element in disease diagnosis, severity assessment, characteristic estimation, and monitoring during the rehabilitation process. Furthermore, it summarizes the state-of-the-art, analyzes challenges, and provides foreseeable future trends for developing IMU-ML systems for IoHT.
The Quality in Psychiatric Care–Inpatient Staff Instrument: A Psychometric Evaluation
Authors: Schröder Agneta , Lundqvist Lars-Olov
Published: June 2022

Much work has focused on the development of instruments that measure the quality of care, but few studies have been published for staff assessment of the quality of care provided by inpatient psychiatric care. Therefore, an instrument is needed to measure the quality of care from the perspective of facility staff. The aim of the present study was to evaluate the psychometric properties and factor structure of the Quality in Psychiatric Care-Inpatient Staff (QPC-IPS) instrument. A sample of 104 staff at seven wards in four regions in Sweden completed the QPC-IPS, which consists of 30 items covering six dimensions of quality. Confirmatory factor analysis confirmed the proposed six factor structure of the QPC-IPS. Internal consistency for the full QPC-IPS was adequate, but poor for some of the dimensions. Staff ratings of the quality of care were generally high. The highest rating was for the Support dimension and the lowest for the Secure environment dimension.
Measuring and Modelling Evaporation Losses from Wet Branches of Lemon Trees
Authors: Baiamonte Giorgio , Palermo Samuel
Published: June 2022

Evaporation losses of rainfall intercepted by canopies depend on many factors, including the temporal scale of observations. At the event scale, interception is a few millimetres, whereas at a larger temporal scale, the number of times that a canopy is filled by rainfall and then depleted can make the interception an important fraction of the rainfall depth. Recently, a simplified interception/evaporation model has been proposed, which considers a modified Merrian model to compute interception during wet spells and a simple power-law equation to model evaporation from wet canopy during dry spells. Modelling evaporation process at the sub hourly temporal scale required the two parameters of the power-law, describing the hourly evaporation depth and the evaporation rate. In this paper, for branches of lemon trees, we focused on the evaporation process from wet branches starting from the interception capacity, S, and simple models in addition to the power-law were applied and tested. In particular, for different temperature, T, and vapour pressure deficit, VPD, conditions, numerous experimental testes were carried out, and the two parameters describing the evaporation process from wet branches were determined and linked to T, VPD and S. The results obtained in this work help us to understand the studied process, highlight its complexity, and could be implemented in the recently introduced interception/evaporation model to quantify this important component of the hydrologic cycle.
Spatial Aggregation and Biometric Variability of the Grass Calamagrostis epigejos (L.) Roth during Different Expansion Stages in Mesic Mountain Meadows
Authors: Pruchniewicz Daniel , Żołnierz Ludwik
Published: June 2022

Calamagrostis epigejos (L.) Roth is one of the most expansive clonal grass species. Despite many publications about its biology, the expansive mechanism of C. epigejos is relatively unknown. Therefore, the aim of this study was to determine: I. the dependency between Calamagrostis epigejos ramet density, habitat properties, and the biometric variability of the species; II. the relation between clone architecture and the diversity parameters and the productivity of grassland biocoenoses; III. the expansion strategy of Calamagrostis epigejos within mesic meadows, representing three stages of degradation. The research was conducted in the Central Sudetes (SW Poland). Ten transects were selected for the study, each representing Arrhenatheretalia-type meadows with patches degraded as a result of Calamagrostis epigejos expansion:initial, intermediate, and advanced. The phalanx strategy was observed within the studied range of the Calamagrostis epigejos expansion in the mesic mountain meadows. The study showed no relation between the Calamagrostis epigejos expansion and the phenomenon of ramet self-thinning, though it noted the influence of the habitat on the variability of its biometric features.
Coordination Models for Cancer Care in Low- and Middle-Income Countries: A Scoping Review
Authors: Lubuzo Buhle , Hlongwana Khumbulani W. , Hlongwa Mbuzeleni , Ginindza Themba G.
Published: June 2022

Background: The coordination of cancer care among multiple providers is vital to improve care quality and ensure desirable health outcomes across the cancer continuum, yet evidence is scarce of this being optimally achieved in low- and middle-income countries (LMICs). Objective: Through this scoping review, our objective was to understand the scope of cancer care coordination interventions and services employed in LMICs, in order to synthesise the existing evidence and identify key models and their elements used to manage and/or improve cancer care coordination in these settings. Methods: A detailed search strategy was conducted, aligned with the framework of Arksey and O’Malley. Articles were examined for evidence of coordination interventions used in cancer care in LMICs. We followed the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) Extension Guidelines for Scoping Reviews, which included a checklist and explanation. The PRISMA flow diagram was utilised to report the screening of results. Data were extracted, categorised and coded to allow for a thematic analysis of the results. Results: Fourteen studies reported on coordination interventions in cancer care in LMICs. All studies reported a positive impact of cancer coordination interventions on the primary outcome measured. Most studies reported on a patient navigation model at different points along the cancer care continuum. Conclusions: An evidence-based and culturally sensitive plan of care that aims to promote coordinated and efficient multidisciplinary care for patients with suspicion or diagnosis of cancer in LMICs is feasible and might improve the quality of care and efficiency.
Effects of a Long-Term Monitored Exercise Program on Aerobic Fitness in a Small Group of Children with Cystic Fibrosis
Authors: Gruber Wolfgang , Stehling Florian , Blosch Christopher , Dillenhoefer Stefanie , Olivier Margarete , Koerner-Rettberg Cordula , Sutharsan Sivagurunathan , Mellies Uwe , Taube Christian , Welsner Matthias
Published: June 2022

Background: The aim of this study was to investigate the effects of a monitored exercise program on aerobic fitness in children with cystic fibrosis (CF). Methods: Six children (2f/4m) with ages ranging from 6 to 14 years (11.3 ± 3.3 years.) and a mean ppFEV1 102.5 ± 13.5% pred. participated in the partially monitored 12-month exercise program. VO2peak and Wpeak were used as parameters of aerobic fitness. Incremental Cardio-Pulmonary Exercise Tests (CPETs) were performed before the program began (T1), after 6 months (T3) of monitoring, and after a further 6 months (T4) without monitoring. Habitual physical activity (HPA) was assessed with accelerometry. Results: The values of VO2peak and Wpeak improved slightly from T1 to T3 (p > 0.05), without a further increase after monitoring was stopped (T4). However, the VO2peak and Wpeak values were higher after monitoring was stopped compared to at T1. The exercise program with and without monitoring (p > 0.05) had no or only a slight effect on the FEV1 values, steps/day, and the intensity of HPA. Conclusions: Monitoring seems to facilitate the achievement of beneficial effects on physical fitness in CF children. For that reason, continuous individual exercise monitoring programs that involve close contact with an exercise therapist should be provided to maintain long-term motivation and participation in physical activities and sport activities during leisure time.
Psychometric Properties of the Spontaneity Assessment Inventory-Revised (SAI-R): The 3-Factor Hypothesis
Authors: Martins Paulo , Gonzalez António-José , Pedroso de Lima Margarida , Rosado António
Published: June 2022

Spontaneity and its connections to mental health and wellbeing are a central issue in both theory and practice of psychodrama, and the specialized literature sometimes even associates the presence of pathology to a lack of spontaneity. This paper describes spontaneity in Moreno’s theory, its assessment, its association with other measures and concepts, and scientific advances in the field. Specifically, we present results obtained with the English and Portuguese-language versions concerning its assessment. The main purpose of this work was to study the spontaneity measure (SAI-R) in a 3 first-order factors version for a Portuguese sample of 439 subjects, divided into two independent samples. The mean age of participants was 25.6 years (SD = 10.2), and ages ranged between 18 and 64 years. Data was collected through an online platform of a Portuguese market research company. The results revealed that the 3-factor model has acceptable validity for items 1 (“Creative”) and 7 (“Euphoric”), confirming the composite reliability, the convergent validity, but not the discriminant validity. Through multi-group analysis, the model proved to be stable. Notwithstanding the need for complementary studies, including clinical samples, the SAI-R is a short and valid instrument in clinical and non-clinical contexts when evaluating spontaneity.
Preliminary Study of the Antimicrobial, Anticoagulant, Antioxidant, Cytotoxic, and Anti-Inflammatory Activity of Five Selected Plants with Therapeutic Application in Dentistry
Authors: López Villarreal Sonia M. , Elizondo Luévano Joel H. , Pérez Hernández Raymundo A. , Sánchez García Eduardo , Verde Star María J. , Castro Ríos Roció , Garza Tapia Marsela , Rodríguez Luis Osvelia E. , Chávez Montes Abelardo
Published: June 2022

The usefulness of traditional plants in Mexico to treat human ailments has been known since ancient times. This work evaluated the antimicrobial, anticoagulant, antioxidant, cytotoxic, and anti-inflammatory potential of ethanolic extracts of Aloe vera, Equisetum arvense, Mimosa tenuiflora, Lippia graveolens, and Syzygium aromaticum. The antimicrobial activity of the extracts was evaluated against Streptococcus mutans and Streptococcus sorbinus; a significant inhibitory effect of the L. graveolens extract on both bacteria was observed at concentration levels of 250 µg/mL and greater. The anticoagulant activity was evaluated in terms of prothrombin time (PT) and activated partial thromboplastin time (APTT), A. vera and M. tenuiflora extracts showed no significant difference (p ˂ 0.05) in PT compared with the control, and for APTT the extracts of A. vera, L. graveolens, and S. aromaticum decreased the APTT significantly (p ˂ 0.05) compared with the control. The antioxidant potential by DPPH assay indicated that the E. arvense extract behaved statistically the same as the control. The cytotoxic activity was evaluated in HGF-1 cells using the fluorometric microculture cytotoxicity assay technique, and none of the extracts was toxic at 125 and 250 µg/mL concentrations. Finally, the anti-inflammatory activity was evaluated using ELISA, where the A. vera extract showed the best anti-inflammatory capacity. Further research on the search for bioactive metabolites and elucidation of action mechanisms of the most promising extracts will be carried out.
Green Economy Development Progress in the Republic of Buryatia (Russia)
Authors: Bilgaev Alexey , Sadykova Erzhena , Mikheeva Anna , Bardakhanova Taisiya , Ayusheeva Svetlana , Li Fujia , Dong Suocheng
Published: June 2022

Under current conditions, the green economy concept has received a comprehensive response in achieving the sustainable development of regions. However, measuring green economic development progress is dynamic, quantitatively characterized by indicators reflecting various aspects. The difficulty lies in a comprehensive environmental sustainability assessment in a context that includes the territory’s environmental, social, and economic factors. The study aimed to assess the progress of the Republic of Buryatia’s (Russia) “green” economic development. The proposed methodology for constructing a composite index is based on five dimensions’ aggregation—resource efficiency, environmental efficiency, environmental quality of life, natural assets, and institutional factors. The composite index helped generalize the complex processes of the region’s environmental–socio–economic development. Its main feature is the reflection of the environmental specificity of the territory. We built a mid-term forecast of the composite and sub-indices, determined their future trend, and assessed the opportunities and conditions for the fastest transition of the Republic of Buryatia to a green economy. The developed composite index is a key tool for regulating green economic development progress, determining prospects, and region management. This paper attempts to fill the gap in a comprehensive assessment of the Republic of Buryatia’s current situation using a composite index.
Psychometric Properties of the Short-Form Geriatric Depression Scale (GDS-SF) and Its Associated Factors among the Elderly in Bangladesh
Authors: Sultana Naznin , Nguyen Thao T. P. , Hossain Ahmed , Asaduzzaman Md. , Nguyen Minh H. , Jahan Ishrat , Nguyen Kien T. , Duong Tuyen Van
Published: June 2022

Background: This study aimed to (1) evaluate the psychometric properties of a Comprehensive Short-Form Geriatric Depression Scale (GDS-SF) and (2) examine the associated factors of GDS-SF among the elderly. Methods: A cross-sectional study was conducted from November 2019 to April 2020 in Dhaka City Corporation, Bangladesh. Data of 377 elderly were collected, including socio-demographic characteristics, social supports, comorbidities, sleep behaviours, and depression (as measured by the GDS-SF). We used the principal component analysis, correlation analysis, and logistic regression analysis to validate GDS-SF, and explore its associations. Results: The GDS-SF was reliable and homogeneous with Cronbach’s alpha = 0.836, and McDonald’s Omega = 0.841, with no floor/ceiling effects. The questionnaire demonstrated a good construct validity with item-scale convergent validity and KMO measure of sampling adequacy (0.869 for the total sample, 0.838 for the community subsample, and 0.851 for the slum subsample). In the multivariate model, older people had a higher likelihood of moderate and severe depression (OR, 1.06; 95% CI, 1.00, 1.12; p = 0.048). The likelihood of having moderate and severe depression was lower in men (OR, 0.48; 95% CI, 0.28, 0.85; p = 0.011) and those satisfied with their children’s support (OR, 0.17; 95% CI, 0.08, 0.35; p < 0.001), compared with their counterparts, respectively. Conclusions: The GDS-SF is a reliable and valid survey tool for evaluating depression in Bangladeshi older adults. Age, gender, and satisfaction with children’s support were predictors of depression.
New Trend of Physical Activity and Exercise for Health Promotion and Functional Ability
Authors: Park Wonil , Park Hun-Young
Published: June 2022

Regular physical activity and exercise can improve your health and reduce your risk of developing various diseases including type 2 diabetes, cardiovascular disease, and cancer [...]
Dynamics of Primary Social Networks to Support Mothers, Fathers, or Guardians of Transgender Children and Adolescents: A Systematic Review
Authors: Abreu Paula Daniella de , Andrade Rubia Laine de Paula , Maza Israel Lucas da Silva , Faria Mariana Gaspar Botelho Funari de , Nogueira Jordana de Almeida , Monroe Aline Aparecida
Published: June 2022

Mothers, fathers, or guardians of children and adolescents who do not identify with the gender they were assigned at birth face barriers in their social network to recognize their children’s gender identity. This study aimed to analyze the scientific evidence on the dynamics of primary social networks to support mothers, fathers, or guardians of transgender children and adolescents. This is a systematic review of qualitative studies guided by the PRISMA guidelines. Controlled and free vocabulary were used to survey the studies in the EMBASE, Scopus, MEDLINE, Cumulative Index to Nursing and Allied Health Literature (CINAHL), PsycInfo, Latin American and Caribbean Literature in Health Sciences (LILACS), and Web of Science databases. A total of 21 studies composed the final sample. Primary social networks were described as fragile and conflicting family/blood relationship ties with disapproval, isolation, rejection, a lack of understanding, and feelings of exclusion were expressed. Some have lost friends, reported tension in marriage and with relatives, and were commonly treated with hostility and harassment. Social transition does take place in the mutual context of struggle and resistance which demands a support network for parents or guardians.
Correlation between Campus-Built Environment and Physical Fitness in College Students in Xi’an—A GIS Approach
Authors: Lu Zijun , Li Zhengao , Mao Chuangui , Tan Yuanyuan , Zhang Xingyue , Zhang Ling , Zhu Wenfei , Sun Yuliang
Published: June 2022

Background: This research aimed to investigate the correlation between students’ physical fitness and campus-built environment, which could put forward some suggestions for the construction of a campus environment. Method: Four colleges in Xi’an were regarded as special “semi-closed” spaces. Combined with ArcGIS and SPSS, the correlation between the built environment of colleges and the students’ physical fitness test results in 2019 was analyzed (n = 1498). Results: regarding the men questioned in this research, there was a significant correlation between street connectivity and vital capacity, grip strength, 50 m running, 1000 m running, a significant correlation between land use mix and vital capacity, sit-and-reach, pull-up, grip strength, a significant correlation between green space per capita and vital capacity, grip strength, 50 m running, and a significant correlation between walk score and vital capacity, pull-up, grip strength, and 50 m running. Regarding the women questioned in this research, there was a significant correlation between street connectivity and vital capacity, grip strength, 50 m running, 800 m running, curl-up, a significant correlation between land use mix and vital capacity, sit-and-reach, curl-up, grip strength, 800 m running, a significant correlation between green space per capita and vital capacity, grip strength, curl-up, sit-and-reach, and a significant correlation between walk score and vital capacity, curl-up, grip strength, and 800 m running. Conclusion: the built environment on campus can indirectly affect the physical fitness of college students. Increasing the number of intersections and short connections of campus streets, ensuring that the green space of the campus meets the standards, and reasonably arranging the site selection of buildings are conducive to improving the physical fitness of students.
Association between Domains of the Clinical-Functional Vulnerability Index and Falls History in Older Adults: A Cross-Sectional Study
Authors: Moreira Natália B. , Bento Paulo C. B. , Vieira Edgar Ramos , da Silva José L. P. , Rodacki André L. F.
Published: June 2022

Objectives: The study aimed to determine which domains, sets, and isolated or combined questions of the Clinical-Functional Vulnerability Index (CFVI-20) are associated with falls history in older adults. Methods: Instruments used were the CFVI-20 assessment and reported falls during the last year. The receiver operating characteristics (ROC) curves identified the performance of the CFVI-20 domains and questions in identifying older adults with and without falls history, while logistic regression identified relevant questions to identify fall history. Results: This study included 1725 individuals (71.9 ± 7.3 years). The area under the curve (AUC) between the CFVI-20 and fall history was 0.69. The mobility domain presented the largest AUC (0.71; p < 0.01), and most isolated domains showed low AUCs (0.51 to 0.58). Isolated questions were limited to identifying fallers. The regression analysis identified 7 questions of the CFVI-20 with falls. Conclusions: The CFVI-20 general score identified older adults with a fall history. When considered in isolation, most domains were limited to identifying falls, except for the mobility domain. Combining the CFVI-20 questions enabled identification of fallers.
Online and Offline Intervention for the Prevention of Postpartum Depression among Rural-to-Urban Floating Women: Study Protocol for a Randomized Control Trial
Authors: Qiu Xichenhui , Li Ting , Fang Qiyu , Huang Lingling , Zheng Xujuan
Published: June 2022

Background: As a higher-risk group of postpartum depression (PPD), rural to urban floating women urgently require effective and accessible mental health care after childbirth to prevent PPD. Even though there were various interventions, only a small number of women have sought professional help to reduce their depressive symptoms after childbirth, suggesting the need for an innovative intervention delivery to overcome women’s help-seeking barriers. Online and offline (OTO) interventions, which combine face-to-face and internet-based interventions, provide apparent benefits. As a result, the protocol for a randomized controlled study (RCT) was designed to examine the effectiveness and acceptability of OTO intervention on psychosocial outcomes for Chinese rural-to-urban floating women including the reduction of PPD symptoms and PPD stigma, and the improvement of social support and quality of life. Methods: A double blind, multicenter, RCT will be used and a total of 226 participants will be recruited. The OTO intervention called the “Hi, Mom” program will integrate two face-to-face consulting sessions with online sessions comprising an information module, a communication module, an ask-the-expert module, and a peer story module over a period of three months. The control group will receive routine postpartum care. Outcome measures including PPD symptoms, PPD stigma, social support, quality of life, mother–child bonding, and satisfaction with health care received will be conducted at baseline, postintervention, and three-month follow-up. Results and Discussion: If the intervention is effective, it will provide a convenient and effective intervention program on postpartum mental well-being for rural-to-urban floating women. As the first study to test the effects of an OTO intervention for the prevention of PPD in China, the outcomes gained from this study will provide evidence-based knowledge for clinical practice on PPD prevention based on online and offline health technologies. Moreover, it could be used to plan a culturally appropriate OTO intervention for migrant mothers from different countries.
Characterization of Atmospheric Fine Particles and Secondary Aerosol Estimated under the Different Photochemical Activities in Summertime Tianjin, China
Authors: Gu Jinxia , Chen Zexin , Zhang Nan , Peng Shitao , Cui Wenjing , Huo Guangyao , Chen Feng
Published: June 2022

In order to evaluate the pollution characterization of PM2.5 (particles with aerodynamic diameters less than or equal to 2.5 μm) and secondary aerosol formation under the different photochemical activity levels, CO was used as a tracer for primary aerosol, and hourly maximum of O3 (O3,max) was used as an index for photochemical activity. Results showed that under the different photochemical activity levels of L, M, LH and H, the mass concentration of PM2.5 were 29.8 ± 17.4, 32.9 ± 20.4, 39.4 ± 19.1 and 42.2 ± 18.9 μg/m3, respectively. The diurnal patterns of PM2.5 were similar under the photochemical activity and they increased along with the strengthening of photochemical activity. Especially, the ratios of estimated secondary aerosol to the observed PM2.5 were more than 58.6% at any hour under the photochemical activity levels of LH and H. The measured chemical composition included water soluble inorganic ions, organic carbon (OC), and element carbon (EC), which accounted for 73.5 ± 14.9%, 70.3 ± 24.9%, 72.0 ± 21.9%, and 65.8 ± 21.2% in PM2.5 under the photochemical activities of L, M, LH, and H, respectively. Furthermore, the sulfate (SO42−) and nitrate (NO3−) were nearly neutralized by ammonium (NH4+) with the regression slope of 0.71, 0.77, 0.77, and 0.75 between [NH4+] and 2[SO42−] + [NO3−]. The chemical composition of PM2.5 was mainly composed of SO42−, NO3−, NH4+ and secondary organic carbon (SOC), indicating that the formation of secondary aerosols significantly contributed to the increase in PM2.5. The formation mechanism of sulfate in PM2.5 was the gas-phase oxidation of SO2 to H2SO4. Photochemical production of nitric acid was intense during daytime, but particulate nitrate concentration was low in the afternoon due to high temperature.
The Role of Alternative Oxidase in the Interplay between Nitric Oxide, Reactive Oxygen Species, and Ethylene in Tobacco (Nicotiana tabacum L.) Plants Incubated under Normoxic and Hypoxic Conditions
Authors: Zafari Somaieh , Vanlerberghe Greg C. , Igamberdiev Abir U.
Published: June 2022

The transgenic tobacco (Nicotiana tabacum L.) plants with the modified levels of alternative oxidase (AOX) were used to evaluate the physiological roles of AOX in regulating nitro-oxidative stress and metabolic changes after exposing plants to hypoxia for 6 h. Under normoxia, AOX expression resulted in the decrease of nitric oxide (NO) levels and of the rate of protein S-nitrosylation, while under hypoxia, AOX overexpressors exhibited higher NO and S-nitrosylation levels than knockdowns. AOX expression was essential in avoiding hypoxia-induced superoxide and H2O2 levels, and this was achieved via higher activities of catalase and glutathione reductase and the reduced expression of respiratory burst oxidase homolog (Rboh) in overexpressors as compared to knockdowns. The AOX overexpressing lines accumulated less pyruvate and exhibited the increased transcript and activity levels of pyruvate decarboxylase and alcohol dehydrogenase under hypoxia. This suggests that AOX contributes to the energy state of hypoxic tissues by stimulating the increase of pyruvate flow into fermentation pathways. Ethylene biosynthesis genes encoding 1-aminocyclopropane-1-carboxylic acid (ACC) synthase, ACC oxidase, and ethylene-responsive factors (ERFs) were induced during hypoxia and correlated with AOX and NO levels. We conclude that AOX controls the interaction of NO, reactive oxygen species, and ethylene, triggering a coordinated downstream defensive response against hypoxia.
Gut Microbiota and Sex Hormones: Crosstalking Players in Cardiometabolic and Cardiovascular Disease
Authors: Maffei Silvia , Forini Francesca , Canale Paola , Nicolini Giuseppina , Guiducci Letizia
Published: June 2022

The available evidence indicates a close connection between gut microbiota (GM) disturbance and increased risk of cardiometabolic (CM) disorders and cardiovascular (CV) disease. One major objective of this narrative review is to discuss the key contribution of dietary regimen in determining the GM biodiversity and the implications of GM dysbiosis for the overall health of the CV system. In particular, emerging molecular pathways are presented, linking microbiota-derived signals to the local activation of the immune system as the driver of a systemic proinflammatory state and permissive condition for the onset and progression of CM and CV disease. We further outline how the cross-talk between sex hormones and GM impacts disease susceptibility, thereby offering a mechanistic insight into sexual dimorphism observed in CVD. A better understanding of these relationships could help unravel novel disease targets and pave the way to the development of innovative, low-risk therapeutic strategies based on diet interventions, GM manipulation, and sex hormone analogues.
Early and Polyantigenic CD4 T Cell Responses Correlate with Mild Disease in Acute COVID-19 Donors
Authors: Tarke Alison , Potesta Marina , Varchetta Stefania , Fenoglio Daniela , Iannetta Marco , Sarmati Loredana , Mele Dalila , Dentone Chiara , Bassetti Matteo , Montesano Carla , Mondelli Mario U. , Filaci Gilberto , Grifoni Alba , Sette Alessandro
Published: June 2022

We assessed SARS-CoV-2-specific CD4+ and CD8+ T cell responses in samples from 89 acute COVID-19 patients, utilizing blood samples collected during the first wave of COVID-19 in Italy. The goal of the study was to examine correlations between SARS-CoV-2-specific T cell responses in the early phase comparing mild, moderate, or severe COVID-19 disease outcomes. T cell responses to the spike (S) and non-S proteins were measured in a combined activation-induced marker (AIM) and intracellular cytokine staining (ICS) assay. Early CD4+ T cell responses to SARS-CoV-2 S correlated with milder disease by both AIM and IFNγ ICS readouts. The correlation of S-specific CD4+ T cell responses with milder disease severity was most striking within the first two weeks of symptom onset compared to later time points. Furthermore, donors with milder disease were associated with polyantigenic CD4+ T cell responses that recognized more prominently non-S proteins in addition to S, while severe acute COVID-19 was characterized by lower magnitudes of CD4+ T cell responses and a narrower repertoire. In conclusion, this study highlights that both the magnitude and breadth of early SARS-CoV-2-specific CD4+ T cell responses correlated with milder disease outcomes in acute COVID-19 patients.
Therapeutic Targeting of Ovarian Cancer Stem Cells Using Estrogen Receptor Beta Agonist
Authors: He Yi , Alejo Salvador , Venkata Prabhakar Pitta , Johnson Jessica D. , Loeffel Ilanna , Pratap Uday P. , Zou Yi , Lai Zhao , Tekmal Rajeshwar R. , Kost Edward R. , Sareddy Gangadhara R.
Published: June 2022

Ovarian cancer (OCa) is the deadliest gynecologic cancer. Emerging studies suggest ovarian cancer stem cells (OCSCs) contribute to chemotherapy resistance and tumor relapse. Recent studies demonstrated estrogen receptor beta (ERβ) exerts tumor suppressor functions in OCa. However, the status of ERβ expression in OCSCs and the therapeutic utility of the ERβ agonist LY500307 for targeting OCSCs remain unknown. OCSCs were enriched from ES2, OV90, SKOV3, OVSAHO, and A2780 cells using ALDEFLUOR kit. RT-qPCR results showed ERβ, particularly ERβ isoform 1, is highly expressed in OCSCs and that ERβ agonist LY500307 significantly reduced the viability of OCSCs. Treatment of OCSCs with LY500307 significantly reduced sphere formation, self-renewal, and invasion, while also promoting apoptosis and G2/M cell cycle arrest. Mechanistic studies using RNA-seq analysis demonstrated that LY500307 treatment resulted in modulation of pathways related to cell cycle and apoptosis. Western blot and RT-qPCR assays demonstrated the upregulation of apoptosis and cell cycle arrest genes such as FDXR, p21/CDKN1A, cleaved PARP, and caspase 3, and the downregulation of stemness markers SOX2, Oct4, and Nanog. Importantly, treatment of LY500307 significantly attenuated the tumor-initiating capacity of OCSCs in orthotopic OCa murine xenograft models. Our results demonstrate that ERβ agonist LY500307 is highly efficacious in reducing the stemness and promoting apoptosis of OCSCs and shows significant promise as a novel therapeutic agent in treating OCa.
Maleic Anhydride-Grafted PLA Preparation and Characteristics of Compatibilized PLA/PBSeT Blend Films
Authors: Jang Hyunho , Kwon Sangwoo , Kim Sun Jong , Park Su-il
Published: June 2022

Poly(butylene sebacate-co-terephthalate) (PBSeT) is a biodegradable flexible polymer suitable for melt blending with other biodegradable polymers. Melt blending with a compatibilizer is a common strategy for increasing miscibility between polymers. In this study, PBSeT polyester was synthesized, and poly(lactic acid) (PLA) was blended with 25 wt% PBSeT by melt processing with 3–6 phr PLA-grafted maleic anhydride (PLA-g-MAH) compatibilizers. PLA-g-MAH enhanced the interfacial adhesion of the PLA/PBSeT blend, and their mechanical and morphological properties confirmed that the miscibility also increased. Adding more than 6 phr of PLA-g-MAH significantly improved the mechanical properties and accelerated the cold crystallization of the PLA/PBSeT blends. Furthermore, the thermal stabilities of the blends with PLA-g-MAH were slightly enhanced. PLA/PBSeT blends with and without PLA-g-MAH were not significantly different after 120 h, whereas all blends showed a more facilitated hydrolytic degradation rate than neat PLA. These findings indicate that PLA-g-MAH effectively improves PLA/PBSeT compatibility and can be applied in the packaging industry.
Antigen Peptide Transporter 1 (TAP1) Promotes Resistance to MEK Inhibitors in Pancreatic Cancers
Authors: Li Boya , Feng Yu , Hou Qiaoyun , Fu Yan , Luo Yongzhang
Published: June 2022

Mitogen-activated protein kinase (MAPK) kinase (MEK) inhibitors show limited benefit in Kirsten rat sarcoma (KRAS) mutant pancreatic cancer due to drug resistance. To identify mechanisms of resistance to MEK inhibitor (MEKi), we employed a differential expression analysis of MEKi-sensitive versus MEKi-resistant KRAS-mutant pancreatic cancer cell lines. Here, we report that the antigen peptide transporter 1 (TAP1) expression levels of MEKi-resistant cell lines were notably higher than those of MEKi-sensitive cell lines. Suppression of TAP1 significantly sensitized the MEKi-resistant pancreatic ductal adenocarcinoma (PDAC) cells to MEKi and induced higher apoptotic rate in vitro. Moreover, knockdown of TAP1 in MEKi-resistant tumor significantly decreased tumor growth in vivo. Consistently, overexpression of TAP1 in sensitive PDAC cells resulted in increased resistance to MEKi, both in vitro and in vivo. Mechanistic studies demonstrated that TAP1 promoted chemoresistance by enhancing the transport of MEKi out of PDAC cells, leading to reduced intracellular MEKi concentration and attenuated inhibition of KRAS signaling pathways. Moreover, TAP1 expression increased spheroid formation abilities of PDAC cells. These findings suggest that TAP1 could serve as a potential marker for predicting the response of patients to MEKi. Combination of TAP1 suppression and MEKi may provide a novel therapeutic strategy for PDAC treatment.
Hydrogen Sulfide Plays an Important Role by Regulating Endoplasmic Reticulum Stress in Diabetes-Related Diseases
Authors: Zhao Huijie , Liu Huiyang , Yang Yihan , Lan Tianyue , Wang Honggang , Wu Dongdong
Published: June 2022

Endoplasmic reticulum (ER) plays important roles in protein synthesis, protein folding and modification, lipid biosynthesis, calcium storage, and detoxification. ER homeostasis is destroyed by physiological and pharmacological stressors, resulting in the accumulation of misfolded proteins, which causes ER stress. More and more studies have shown that ER stress contributes to the pathogenesis of many diseases, such as diabetes, inflammation, neurodegenerative diseases, cancer, and autoimmune diseases. As a toxic gas, H2S has, in recent years, been considered the third most important gas signal molecule after NO and CO. H2S has been found to have many important physiological functions and to play an important role in many pathological and physiological processes. Recent evidence shows that H2S improves the body’s defenses to many diseases, including diabetes, by regulating ER stress, but its mechanism has not yet been fully understood. We therefore reviewed recent studies of the role of H2S in improving diabetes-related diseases by regulating ER stress and carefully analyzed its mechanism in order to provide a theoretical reference for future research.