bluuebunny/crossref_metadata_2025 huggingface dataset:
Chronic Sleep Deprivation Blocks Voluntary Morphine Consumption but Not Conditioned Place Preference in Mice
Authors: Eacret Darrell , Lemchi Crystal , Caulfield Jasmine I. , Cavigelli Sonia A. , Veasey Sigrid C. , Blendy Julie A.
Published: February 2022

The opioid epidemic remains a significant healthcare problem and is attributable to over 100,000 deaths per year. Poor sleep increases sensitivity to pain, impulsivity, inattention, and negative affect, all of which might perpetuate drug use. Opioid users have disrupted sleep during drug use and withdrawal and report poor sleep as a reason for relapse. However, preclinical studies investigating the relationship between sleep loss and substance use and the associated underlying neurobiological mechanisms of potential interactions are lacking. One of the most common forms of sleep loss in modern society is chronic short sleep (CSS) (<7 h/nightly for adults). Here, we used an established model of CSS to investigate the influence of disrupted sleep on opioid reward in male mice. The CSS paradigm did not increase corticosterone levels or depressive-like behavior after a single sleep deprivation session but did increase expression of Iba1, which typically reflects microglial activation, in the hypothalamus after 4 weeks of CSS. Rested control mice developed a morphine preference in a 2-bottle choice test, while mice exposed to CSS did not develop a morphine preference. Both groups demonstrated morphine conditioned place preference (mCPP), but there were no differences in conditioned preference between rested and CSS mice. Taken together, our results show that recovery sleep after chronic sleep disruption lessens voluntary opioid intake, without impacting conditioned reward associated with morphine.
Maternal Pre-pregnancy Body Mass Index Categories and Infant Birth Outcomes: A Population-Based Study of 9 Million Mother–Infant Pairs
Authors: Zong Xin'nan , Wang Huan , Yang Liu , Guo Yajun , Zhao Min , Magnussen Costan G. , Xi Bo
Published: February 2022

Background and Aims

Infant adverse birth outcomes have been suggested to contribute to neonatal morbidity and mortality and may cause long-term health consequences. Although evidence suggests maternal prepregnancy body mass index (BMI) categories associate with some birth outcomes, there is no consensus on these associations. We aimed to examine the associations of maternal prepregnancy BMI categories with a wide range of adverse birth outcomes.

Methods

Data were from a population-based retrospective cohort study of 9,282,486 eligible mother–infant pairs in the U.S. between 2016 and 2018. Maternal prepregnancy BMI was classified as: underweight (<18.5 kg/m2); normal weight (18.5–24.9 kg/m2); overweight (25.0–29.9 kg/m2); obesity grade 1 (30–34.9 kg/m2); obesity grade 2 (35.0–39.9 kg/m2); and obesity grade 3 (≥40 kg/m2). A total of six birth outcomes of the newborn included preterm birth, low birthweight, macrosomia, small for gestational age (SGA), large for gestational age (LGA), and low Apgar score (5-min score <7).

Results

Maternal prepregnancy overweight and obesity increased the likelihood of infant preterm birth, with odds ratios (ORs) (95% CIs) of 1.04 (1.04–1.05) for overweight, 1.18 (1.17–1.19) for obesity grade 1, 1.31 (1.29–1.32) for obesity grade 2, and 1.47 (1.45–1.48) for obesity grade 3, and also for prepregnancy underweight (OR = 1.32, 95% CI = 1.30–1.34) after adjusting for all potential covariates. Prepregnancy overweight and obesity were associated with higher odds of macrosomia, with ORs (95% CIs) of 1.53 (1.52–1.54) for overweight, 1.92 (1.90–1.93) for obesity grade 1, 2.33 (2.31–2.35) for obesity grade 2, and 2.87 (2.84–2.90) for obesity grade 3. Prepregnancy overweight and obesity was associated with higher odds of LGA, with ORs (95% CIs) of 1.58 (1.57–1.59) for overweight, 2.05 (2.03–2.06) for obesity grade 1, 2.54 (2.52–2.56) for obesity grade 2, and 3.17 (3.14–3.21) for obesity grade 3. Prepregnancy overweight and obesity were also associated with higher odds of low Apgar score, with ORs (95% CIs) of 1.12 (1.11–1.14) for overweight, 1.21 (1.19–1.23) for obesity grade 1, 1.34 (1.31–1.36) for obesity grade 2, and 1.55 (1.51–1.58) for obesity grade 3.

Conclusion

Our findings suggest maintaining or obtaining a healthy body weight for prepregnancy women could substantially reduce the likelihood of important infant adverse birth outcomes.
Mesenchymal Stem Cells Inhibits Migration and Vasculogenic Mimicry in Nasopharyngeal Carcinoma Via Exosomal MiR-125a
Authors: Wan Fangzhu , Zhang Haojiong , Hu Jiyi , Chen Li , Geng Shikai , Kong Lin , Lu Jiade J.
Published: February 2022

Vasculogenic mimicry (VM) is a kind of tumor vasculature providing blood supply for tumor growth, and the formation of VM is independent of vascular endothelial cells. Instead, VM structures are formed by differentiated tumor cells such as nasopharyngeal carcinoma cells. Recently, studies have shown that anti-angiogenic therapy failed to improve the overall survival for patients, namely, nasopharyngeal carcinoma patients. The existence of VM structure is probably one of the reasons for resistance for anti-angiogenic therapy. Therefore, it is important to study the mechanism for VM formation in nasopharyngeal carcinoma. In this study, the bioinformatic analysis revealed that microRNA-125a-3p (miR-125a) was highly expressed in normal nasopharyngeal epithelial tissue than in nasopharyngeal carcinoma. An in vitro study demonstrated that miR-125a plays an inhibitory role in nasopharyngeal carcinoma cell migration and VM formation, and further studies confirmed that TAZ is a direct downstream target for miR-125a. On this basis, we artificially engineered human mesenchymal stem cells (MSCs) to generate exosomes with high miR-125a expression. Treatment with these miR-125a-over-expressing exosomes attenuated the migration and VM formation in nasopharyngeal carcinoma cells. In addition, the inhibitory role of these exosomes on VM formation and migration in nasopharyngeal carcinoma was also confirmed in vivo. Overall, the current study shows that MSCs can be utilized to generate exosomes with high miR-125a level, which could be therapeutic nanoparticles targeting VM formation in nasopharyngeal carcinoma and used as a complement to anti-angiogenic therapy in the future.
The Effect of Erythropoietin and Its Derivatives on Ischemic Stroke Therapy: A Comprehensive Review
Authors: Ma Yuanyuan , Zhou Zhiyuan , Yang Guo-Yuan , Ding Jing , Wang Xin
Published: February 2022

Numerous studies explored the therapeutic effects of erythropoietin (EPO) on neurodegenerative diseases. Few studies provided comprehensive and latest knowledge of EPO treatment for ischemic stroke. In the present review, we introduced the structure, expression, function of EPO, and its receptors in the central nervous system. Furthermore, we comprehensively discussed EPO treatment in pre-clinical studies, clinical trials, and its therapeutic mechanisms including suppressing inflammation. Finally, advanced studies of the therapy of EPO derivatives in ischemic stroke were also discussed. We wish to provide valuable information on EPO and EPO derivatives’ treatment for ischemic stroke for basic researchers and clinicians to accelerate the process of their clinical applications.
An RRx-001 Analogue With Potent Anti-NLRP3 Inflammasome Activity but Without High-Energy Nitro Functional Groups
Authors: Lin Hualong , Yang Mingyang , Li Cong , Lin Bolong , Deng Xianming , He Hongbin , Zhou Rongbin
Published: February 2022

NLRP3 inflammasome is involved in the pathology of multiple human inflammatory diseases but there are still no clinically available medications targeting the NLRP3 inflammasome. We have previously identified RRx-001 as a highly selective and potent NLRP3 inhibitor, however, it contains high-energy nitro functional groups and may cause potential processing problems and generates highly toxic oxidants. Here, we show that compound 149-01, an RRx-001 analogue without high-energy nitro functional groups, is a potent, specific and covalent NLRP3 inhibitor. Mechanistically, 149-01 binds directly to cysteine 409 of NLRP3 to block the NEK7-NLRP3 interaction, thereby preventing NLRP3 inflammasome complex assembly and activation. Furthermore, treatment with 149-01 effectively alleviate the severity of several inflammatory diseases in mice, including lipopolysaccharide (LPS)-induced systemic inflammation, monosodium urate crystals (MSU)-induced peritonitis and experimental autoimmune encephalomyelitis (EAE). Thus, our results indicate that 149-01 is a potential lead for developing therapeutic agent for NLRP3-related inflammatory diseases.
Adjuvant Chemotherapy in pT2N0M0 Gastric Cancer: Findings From a Retrospective Study
Authors: Mei Yu , Feng Xijia , Feng Tienan , Yan Min , Zhu Zhenggang , Li Tian , Zhu Zhenglun
Published: February 2022

Background: There is no global consensus on adjuvant chemotherapy (ACT) for pT2N0M0 gastric cancer. We conducted a retrospective study to reveal the role of ACT in such patients.

Methods: Patients with pT2N0M0 gastric cancer who underwent radical resection with D2 lymphadenectomy for primary gastric cancer between January 2012 and May 2016 were included. Kaplan–Meier and Cox regression were used to evaluate overall survival (OS), disease-specific survival (DSS) and predictors of prognosis. Stratified analysis based on high-risk factors was conducted.

Results: Of enrolled 307 patients, 111 patients underwent surgery alone and 196 patients received ACT. Surgery alone (HR = 2.913, 95% CI: 1.494-5.682, p = 0.002) and total gastrectomy (HR = 2.445, 95% CI: 1.279-4.675, p = 0.007) were independently associated with decreased OS. With the median follow-up of 73.1 months, the 5-year OS rate was 87.9% and 5-year DSS rate was 91.8%. Patients receiving ACT showed a better 5-year OS rate (92.9 vs. 79.3%, p < 0.001) and DSS rate (96.8 vs. 83.0%, p < 0.001) than patients underwent surgery alone. Patients receiving monotherapy (n = 130) had a relatively poor prognosis compared to patients receiving dual-drug (n = 66) without a significant difference (92.3 vs. 93.9%, p = 0.637). In patients without high-risk factors based on the Chinese Society of Clinical Oncology (CSCO) Guidelines, ACT also provided survival benefit (96.0 vs 82.9%, p = 0.038).

Conclusions: ACT was accompanied with higher 5-year OS and DSS rates of patients with pT2N0M0 gastric cancer. Patients with pT2N0M0 gastric cancer, regardless of high-risk factors based on the CSCO guidelines, might be considered candidates for ACT. In regard to the therapy regimen, monotherapy might be the optimal choice, considering the adverse events.
Proportional Cerebellum Size Predicts Fear Habituation in Chickens
Authors: Stingo-Hirmas Diego , Cunha Felipe , Cardoso Rita France , Carra Laura G. , Rönnegård Lars , Wright Dominic , Henriksen Rie
Published: February 2022

The cerebellum has a highly conserved neural structure across species but varies widely in size. The wide variation in cerebellar size (both absolute and in proportion to the rest of the brain) among species and populations suggests that functional specialization is linked to its size. There is increasing recognition that the cerebellum contributes to cognitive processing and emotional control in addition to its role in motor coordination. However, to what extent cerebellum size reflects variation in these behavioral processes within species remains largely unknown. By using a unique intercross chicken population based on parental lines with high divergence in cerebellum size, we compared the behavior of individuals repeatedly exposed to the same fear test (emergence test) early in life and after sexual maturity (eight trials per age group) with proportional cerebellum size and cerebellum neural density. While proportional cerebellum size did not predict the initial fear response of the individuals (trial 1), it did increasingly predict adult individuals response as the trials progressed. Our results suggest that proportional cerebellum size does not necessarily predict an individual’s fear response, but rather the habituation process to a fearful stimulus. Cerebellum neuronal density did not predict fear behavior in the individuals which suggests that these effects do not result from changes in neuronal density but due to other variables linked to proportional cerebellum size which might underlie fear habituation.
Cultural Context or Generational Cohort: Which Influences Tourist Behavior More?
Authors: Pérez-Tapia Gema , Mercadé-Melé Pere , Yeong-Hyeon Hwang , Almeida-García Fernando
Published: February 2022

According to most academics, different generations share common characteristics. This undoubtedly helps to better understand their behavior in different scenarios, predicting their responses. However, this seems questionable and that is the main purpose of this study. This research, although preliminary, try to confirm if millennials have common characteristics, or if, on the contrary, there are differences between them due to the culture in which they are immersed. To this end, it has been contextualized in a sector that is very sensitive to cultural differences, such as tourism. Data collection was carried out through a questionnaire administered in 2019 via e-mail to young South Korean and Spanish millennials (born 1982–2002). The results suggest that there do not appear to be common personality traits between the countries analyzed. The differences between the two groups for most personality traits and travel motivations were significant. The findings of this research provide several practical implications since it will make possible a more appropriate management of the tourist destination.
A Clinical Paradigm for Listening Effort Assessment in Middle-Aged Listeners
Authors: Kaplan Neeman Ricky , Roziner Ilan , Muchnik Chava
Published: February 2022

Listening effort (LE) has been known to characterize speech recognition in noise regardless of hearing sensitivity and age. Whereas the behavioral measure of dual-task paradigm effectively manifests the cognitive cost that listeners exert when processing speech in background noise, there is no consensus as to a clinical procedure that might best express LE. In order to assess the cognitive load underlying speech recognition in noise and promote counselling for coping strategies, a feasible clinical paradigm is warranted. The ecological validity of such a paradigm might best be demonstrated in middle-aged adults, exhibiting intact hearing sensitivity on one hand, however, experiencing difficulties in degraded listening conditions, unaware of the implicated cognitive cost of speech recognition in noise. To this end, we constructed a dual-task paradigm that consists of a primary task of sentences-in-noise recognition and a secondary task of simple visual colored-shape matching. Research objective was to develop a clinical paradigm for the assessment of LE in middle-aged adults. Participants were 17 middle-aged adults (mean age of 52.81 years) and 23 young adults (mean age of 24.90 years). All participants had normal hearing according to age. Speech stimuli consisted of the Hebrew Matrix sentences in noise test. SRTn was obtained for 80% correct identification. Visual stimuli were colored geometric shapes. Outcome measures were obtained initially for each task separately, to establish performance ability, and then obtained simultaneously. Reaction time and accuracy in the secondary task were the defined metrics for LE. Results: LE was indicated for both groups, however, was more pronounced in the middle-aged, manifested in the visual accuracy and reaction time metrics. Both groups maintained the 80% correct recognition-in-noise in the dual-task, however, the middle-aged group necessitated a better SNR of 1.4dB than the normal hearing group. Moreover, the middle-aged group was taxed in a greater prolongation of reaction time, in order to uphold the correct recognition. Conclusion: a dual-task paradigm consisting of sentences-in-noise primary task combined with a simple secondary task successfully showed different manifestations of LE in middle-aged adults compared to young adults, thus approximating the use of such a paradigm in a clinical setting.
Boys Don't Cry: Examining Sex Disparities in Behavioral Oncology Referral Rates for AYA Cancer Patients
Authors: Kivlighan Martin , Bricker Joel , Aburizik Arwa
Published: February 2022

Psychosocial distress is highly prevalent in cancer patients, approaching rates around 40% across various cancer sites according to multicenter studies. As such, distress screening procedures have been developed and implemented to identify and respond to cancer patients' psychosocial distress and concerns. However, many cancer patients continue to report unmet psychosocial needs suggesting gaps in connecting patients with psychosocial services. Presently, there is a paucity of research examining sex-based disparities in referral rates to behavioral oncology services, particularly for adolescent and young adult (AYA) cancer patients. Informed by gender role conflict and empirical literature documenting disparities in cancer care and treatment based on a variety of sociocultural variables, this study aimed to examine the presence of sex disparities in referral rates to behavioral oncology services for AYA cancer patients. Data for this study consisted of 1,700 AYA cancer patients (age 18–39) who completed a distress screening at a large cancer center of a teaching hospital in the Midwestern United. Results indicated that patient sex significantly predicted the odds of behavioral oncology referral (γ50 = −0.95, Odds ratio = 2.60, p < 0.001). This finding indicates that female AYA cancer patients are 2.5 times more likely to be referred to behavioral oncology services compared to male AYA cancer patients after controlling for psychosocial distress and emotional, family, and practical problems. Additionally, we found that emotional problems significantly moderated the odds of referral for males and females (γ60 = 0.37, Odds ratio = 1.44, p < 0.001), however the odds of referral for males who endorsed emotional problems were lower than males who did not endorse emotional problems. This contrasted with female AYA cancer patients where the endorsement of emotional problems increased the odds of referral to behavioral oncology services. Findings are discussed with particular focus on how to enhance equitable care and reduce sex and other sociocultural-based disparities in AYA psychosocial oncology.
Associations Between a Polymorphism in the Rat 5-HT1A Receptor Gene Promoter Region (rs198585630) and Cognitive Alterations Induced by Microwave Exposure
Authors: Li Haijuan , Gao Yu , Zou Yong , Qiao Simo , Zhi Weijia , Ma Lizhen , Xu Xinping , Zhao Xuelong , Zhang Junhua , Wang Lifeng , Hu Xiangjun
Published: February 2022

The nervous system is a sensitive target of electromagnetic radiation (EMR). Chronic microwave exposure can induce cognitive deficits, and 5-HT system is involved in this effect. Genetic polymorphisms lead to individual differences. In this study, we evaluated whether the single-nucleotide polymorphism (SNP) rs198585630 of 5-HT1A receptor is associated with cognitive alterations in rats after microwave exposure with a frequency of 2.856 GHz and an average power density of 30 mW/cm2. Rats were exposed to microwaves for 6 min three times a week for up to 6 weeks. PC12 cells and 293T cells were exposed to microwaves for 5 min up to 3 times at 2 intervals of 5 min. Transcriptional activity of 5-HT1A receptor promoter containing rs198585630 C/T allele was determined in vitro. Electroencephalograms (EEGs), spatial learning and memory, and mRNA and protein expression of 5-HT1A receptor were evaluated in vivo. We demonstrated that transcriptional activity of 5-HT1A receptor promoter containing rs198585630 C allele was higher than that of 5-HT1A receptor promoter containing T allele. The transcriptional activity of 5-HT1A receptor promoter was stimulated by 30 mW/cm2 microwave exposure, and rs198585630 C allele was more sensitive to microwave exposure, as it showed stronger transcriptional activation. Rats carrying rs198585630 C allele exhibited increased mRNA and protein expression of 5-HT1A receptor and were more susceptible to 30 mW/cm2 microwave exposure, showing cognitive deficits and inhibition of brain electrical activity. These findings suggest SNP rs198585630 of the 5-HT1A receptor is an important target for further research exploring the mechanisms of hypersensitivity to microwave exposure.
Incorporating Code Structure and Quality in Deep Code Search
Authors: Yu Hao , Zhang Yin , Zhao Yuli , Zhang Bin
Published: February 2022

Developers usually search for reusable code snippets to improve software development efficiency. Existing code search methods, including methods based on full-text or deep learning, have two disadvantages: (1) ignoring structural information of code snippets, such as conditional statements and loop statements, and (2) ignoring quality information of code snippets, such as naming clarity and logical correctness. These disadvantages limit the performance of existing code search methods. In this paper, we propose a novel code search method named Structure and Quality based Deep Code Search (SQ-DeepCS). SQ-DeepCS introduces a code representation method called program slice to represent structual information as well as API usage of code snippets. Meanwhile, SQ-DeepCS introduces a novel deep neural network named Method-Description-Joint Embedding Neural Network (MD-JEnn) to weight the quality of code snippets. To evaluate the proposed methods, we train MD-JEnn and evaluate SQ-DeepCS by searching for code snippets with respect to the top-rated questions from Stack Overflow. We use four evaluation indicators to measure the effectiveness of SQ-DeepCS: FRank, SuccessRate@k, PrecisionRate@k, and Mean Reciprocal Rank (MRR). The experimental results show that our approach can provide better results than existing techniques when searching for relevant code snippets.
Learning Competitive Swarm Optimization
Authors: Borowska Bożena
Published: February 2022

Particle swarm optimization (PSO) is a popular method widely used in solving different optimization problems. Unfortunately, in the case of complex multidimensional problems, PSO encounters some troubles associated with the excessive loss of population diversity and exploration ability. This leads to a deterioration in the effectiveness of the method and premature convergence. In order to prevent these inconveniences, in this paper, a learning competitive swarm optimization algorithm (LCSO) based on the particle swarm optimization method and the competition mechanism is proposed. In the first phase of LCSO, the swarm is divided into sub-swarms, each of which can work in parallel. In each sub-swarm, particles participate in the tournament. The participants of the tournament update their knowledge by learning from their competitors. In the second phase, information is exchanged between sub-swarms. The new algorithm was examined on a set of test functions. To evaluate the effectiveness of the proposed LCSO, the test results were compared with those achieved through the competitive swarm optimizer (CSO), comprehensive particle swarm optimizer (CLPSO), PSO, fully informed particle swarm (FIPS), covariance matrix adaptation evolution strategy (CMA-ES) and heterogeneous comprehensive learning particle swarm optimization (HCLPSO). The experimental results indicate that the proposed approach enhances the entropy of the particle swarm and improves the search process. Moreover, the LCSO algorithm is statistically and significantly more efficient than the other tested methods.
Missing Value Imputation Method for Multiclass Matrix Data Based on Closed Itemset
Authors: Tada Mayu , Suzuki Natsumi , Okada Yoshifumi
Published: February 2022

Handling missing values in matrix data is an important step in data analysis. To date, many methods to estimate missing values based on data pattern similarity have been proposed. Most previously proposed methods perform missing value imputation based on data trends over the entire feature space. However, individual missing values are likely to show similarity to data patterns in local feature space. In addition, most existing methods focus on single class data, while multiclass analysis is frequently required in various fields. Missing value imputation for multiclass data must consider the characteristics of each class. In this paper, we propose two methods based on closed itemsets, CIimpute and ICIimpute, to achieve missing value imputation using local feature space for multiclass matrix data. CIimpute estimates missing values using closed itemsets extracted from each class. ICIimpute is an improved method of CIimpute in which an attribute reduction process is introduced. Experimental results demonstrate that attribute reduction considerably reduces computational time and improves imputation accuracy. Furthermore, it is shown that, compared to existing methods, ICIimpute provides superior imputation accuracy but requires more computational time.
The Complexity of Logistics Services at Transshipment Terminals
Authors: Filina-Dawidowicz Ludmiła , Kostrzewski Mariusz
Published: February 2022

Transshipment is the process of off-loading an intermodal loading unit (for example, different types of containers, semitrailers, swap-bodies, and so on) from one means of transport (for example, a vessel, a freight railcar, etc.) and loading it onto another. Such a process, as well as other logistics services related to loading units, may take place at a transshipment terminal, which is the intermediate node added to an intermodal transport network when combining two or more liner services that facilitate freight transport. Growing customer requirements affect transshipment terminal operations and contribute to the development of comprehensive logistics services. Terminal clients expect the delivery of complex services that often pose serious challenges to terminals providing these services. The specific decision-making tools are essential for facilitating the shaping of terminals’ complex service offerings. In this study, we investigated the issues connected to the complexity of logistics services offered by transshipment terminals. The aim was to develop a decision-making approach to assess the complexity of logistics services offered by these terminals. A procedure for the formulation of complex and comprehensive service sets at transshipment terminals, which includes sustainable energy and energy efficiency issues, was proposed. The approach for assessing the complexity of services at terminals handling intermodal loading units was developed, and an appropriate mathematical model was applied. Consequently, indexes of the efficiency and comprehensiveness levels of ordered services in a terminal were proposed. The ranking of decision-making criteria influencing the shaping of complex service offerings was created based on the results of a questionnaire survey distributed among the managerial representatives of terminals located in Poland. The data obtained with the use of a questionnaire survey allowed us to verify the proposed approach. The research results may be useful for the management of transshipment terminals while making decisions on the creation of comprehensive services offered to their clientele.
Hardware and Software Implementation for Solar Hot Water System in Northern Regions of Russia
Authors: Tsvetkov Nikolay , Boldyryev Stanislav , Shilin Aleksandr , Krivoshein Yuriy , Tolstykh Aleksandr
Published: February 2022

Acceleration of energy transition will become the crucial social, political and technical challenge of the 21st century and will be largely associated with the growing use of renewable energy sources, including solar power. This study provides some experimental results of using solar hot water systems (HWS) embedded in apartment buildings located in the energy-efficient district called Zhatay in Yakutsk city, in the Republic of Sakha (Yakutia). The low annual solar fraction of HWS was found for 2019. It is equal to 0.2869 and caused by thermal energy loss from the hydraulic circuit during the nighttime. The study suggests increasing solar fraction in HWS and implementing a software and hardware system. The experimental evaluation of these studies was performed by testing a solar water heating pilot plant in Kaftanchikovo village in Tomsk Region (Western Siberia). As a result of HWS testing, it was found that the annual solar fraction can be significantly increased by preventing the heating agent from night freezing in hydraulic circuits of tube collectors, even when the outdoor temperature is below its freezing point.
The Use of Geomaterials to Restore the Utility Value of Post-Mining Areas
Authors: Perżyło Dagmara , Szafulera Katarzyna , Kruczkowski Marek , Pilch Michał
Published: February 2022

Post-mining deformations that occur on the surface pose a significant threat to natural environments and urbanized areas. Preventing the effects of deformation is a significant challenge for specialists in geotechnical and civil engineering. Geomaterials, such as geosynthetics or geopolymers, could minimize the damage that occurs. The first section of the article explores the securing of an area, strengthening the rock mass with geosynthetic materials. We provide descriptions of the properties of these materials and the method surrounding their introduction into the soil. The second section presents the research problem, i.e., we describe the damage caused by underground mining. In the last section, we propose a solution for securing the ground with the use of geogrids and geopolymer injections into the rock mass. The analyses led us to conclude that an area subjected to mining influences may be strengthened by the use of geosynthetic materials. The use of geosynthetics in a mining area is a well-known topic, but the additional use of geopolymers may be innovative. Research is still being conducted on the use of geopolymers to fill post-mining voids, in combination with geosynthetics.
A Review of the Latest Trends in the Use of Green Ammonia as an Energy Carrier in Maritime Industry
Authors: Mallouppas George , Ioannou Constantina , Yfantis Elias Ar.
Published: February 2022

This review paper examines the key barriers to using green ammonia as an alternative fuel in maritime industry. A literature survey is performed based on research articles and grey literature, with the aim of discussing the technoeconomic problems with and benefits of ammonia and the relevant technologies. The limitations of ammonia as a maritime fuel and its supply chain, the expected percentage demand by 2030 and 2050, its economic performance compared to other shipping fuels such as hydrogen, and the current regulations that may impact ammonia as a maritime fuel are discussed. There are several key barriers to ammonia’s wide adoption: (1) High production costs, due to the high capital costs associated with ammonia’s supply chain; (2) availability, specifically the limited geographical locations available for ammonia bunkering; (3) the challenge of ramping up current ammonia production; and (4) the development of ammonia-specific regulations addressing issues such as toxicity, safety, and storage. The general challenges involved with blue ammonia are the large energy penalty and associated operational costs, and a lack of technical expertise on its use. Regardless of the origin, for ammonia to be truly zero-carbon its whole lifecycle must be considered—a key challenge that will aid in the debate about whether ammonia holds promise as a zero-carbon maritime fuel.
Phenolics Profile and Protective Effect on Injuried HUVEC Cells of Epicarp Extracts from Kadsura coccinea
Authors: Lu Jun , Zheng Ying , Yang Zhenyu , Cheng Jing , Luo Feijun
Published: February 2022

This study evaluated the phenolics profile and the antioxidative properties of K. coccinea fruits epicarp. A total of 13 phenolic compounds (six phenolic acids, four anthocyanins, two flavonols, and one flavone) were identified by ultra performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spetrometry(UPLC-QTOF-MS/MS). Two anthocyanins, cyanidin-3-xylosylrutinoside and cyanidin-3-rutinoside, comprise 30.89~74.76% and 13.90~46.17% of the total amount of anthocyanins in K. Coccinea. Cytoprotective effect results evidenced that pretreatment of Human umbilical vein endothelial cells(HUVECs) with Kadsura. coccinea fruits’ epicarp phenolic extracts at the concentrations of 50–200 µg/mL improved the cell viability after exposure to H2O2 significantly, and inhibited malonaldehyde(MDA) and reactive oxygen species(ROS) overproduction, as well as enhancing the content of superoxide dismutase (SOD) and glutathione Reductase (GR. This study proved that K. coccinea is a natural resource of phenolics rich with potential antioxidant ability, which may be valuable for developing nutraceuticals and dietary supplements.
Plant Adaptability and Vegetation Differentiation in the Coastal Beaches of Yellow–Bohai Sea in China
Authors: Dong Qian , Zhang Qingqing , Liao Anbang , Xu Chi , Liu Maosong
Published: February 2022

To identify the key soil factors influencing the vegetation differentiation in the coastal tidal flats of the Yellow−Bohai Sea in China, this study investigated the corresponding relationship between the Spartina alterniflora (SA), Suaeda salsa (SS), and Phragmites australis (PA) communities and their respective soil factors with published data, and combined the ecological strategy for analysis. The results showed a corresponding relationship between community and soil factors. The SA community had a lower bulk density (BD) and higher soil total nitrogen (TN), and the SS community was the opposite, while the PA community had the lowest salinity and higher TN. BD, salinity and TN acted as the main soil factors driving vegetation differentiation, but the explained proportion of the three factors to vegetation differentiation changed by season and region. Considering that higher TN facilitates the competitors, salinity represents the environmental stresses, and BD is positively related to the frequency of perturbation in the specific habitat in the study area, SA, SS and PA could be recognized as C–S, S–R and C strategic species to some extent. It is likely that some coexistent mechanisms for invasive and local species will be developed, especially the SS community which seriously shrunk recently but served as an important habitat for waterfowls in tidal flat habitats.
Assessment of Physical Activity and Related Factors among Adults with Visual Impairments in Japan
Authors: Tatsuta Wakako , Inayama Takayo , Yamanaka Erika , Ohkawara Kazunori
Published: February 2022

In Japan, there is a lack of physical activity data on health and disease among people with visual impairments, making it difficult to develop specific strategies for health promotion. This study aimed to identify moderate-to-vigorous physical activity (MVPA) among people with visual impairments in Japan, to examine the percentage of them who meet the MVPA recommended activity, and to identify factors related to MVPA. In this cross-sectional study, we analyzed a survey of 169 adults with visual impairments. The relationship between MVPA and related factors was analyzed using binomial logistic regression analysis. The median MVPA was 46 min/day for men and 36 min/day for women, respectively, aged < 65 years, and 26 min/day for men and 34 min/day for women, respectively, aged ≥ 65 years. Seventy-eight percent of the subjects achieved the recommendations by the WHO, and 28% of the subjects < 65 years had achieved the MVPA of 60 min/day recommended by the Japanese Active Guide. Self-efficacy (SE) for PA, recommended PA implementation behavior, subjective walking speed, and exercise habits were significantly associated with MVPA. The current status of MVPA time among people with visual impairments in Japan, as revealed by this study, indicates that it is important to set realistic goals and plan a step-by-step process to achieve the recommendation. In the future, it will be important to develop a program that encourages the improvement of SE and promotes PA.
Changes in Physical Activity Patterns Due to the Covid-19 Pandemic: A Systematic Review and Meta-Analysis
Authors: Wunsch Kathrin , Kienberger Korbinian , Niessner Claudia
Published: February 2022

With the outbreak of the Corona Virus Disease 19 (Covid-19) in late 2019, governments increasingly imposed containment strategies, including social distancing as well as restricted population movement, potentially having negative impacts on mental and physical health. A growing number of studies have examined the impact of the pandemic on different facets of physical activity (PA); an overview combining these (mixed) results, however, is missing. Thus, the objective of this systematic review and meta-analysis was to investigate whether and to which extent PA changed from before to during the Covid-19 pandemic, taking age, gender, and measurement method into account. The literature search was conducted using PubMed, Web of Science, and Scopus. Results of the main characteristics were descriptively synthesized and analyzed in a meta-analysis quantifying effects of the pandemic on PA divided by age groups, with additional subgroup analyses of the characteristics age, gender, and measurement method being narratively synthesized. Overall, 57 studies with a total sample size of 119,094 participants (N between 10 and 60,560 subjects) from 14 countries worldwide with participants aged between four and 93 years were included. Thirty-two studies revealed a significant decline in PA, whereas only five studies found a significant increase in PA during the Covid-19 pandemic. Fourteen studies revealed mixed results. PA decreased in all age groups, independent of gender. Most self-reported and all device-based measurement methods showed a reduction in PA. However, effects were not found to be significant in all age groups. Nevertheless, the declining trend should be noted and governments should strive to enable PA within periods of pandemic restrictions, or promote alternatives such as digital training to avoid negative health consequences within the population.
Predictive and Diagnostic Value of Serum Adipokines in Pregnant Women with Intrahepatic Cholestasis
Authors: Yurtcu Nazan , Caliskan Canan Soyer , Guvey Huri , Celik Samettin , Hatirnaz Safak , Tinelli Andrea
Published: February 2022

The objective of this study was to assess the value of serum leptin, adiponectin, apelin, and ghrelin as biomarkers for the prediction and diagnosis of intra-hepatic cholestasis (ICP). This prospective study included pregnant women in the third trimester of pregnancy: 63 with ICP, 48 and 15 of whom had mild and severe disease, respectively, and 32 as controls. ICP women had increased median levels of serum leptin, adiponectin, apelin, and ghrelin compared to the controls (p < 0.05). These biomarkers meaningfully changed regarding the severity of ICP: While leptin was reduced, apelin and ghrelin were increased, and adiponectin was increased somewhat. To predict and diagnose ICP, the predictive values of serum leptin, adiponectin, and apelin need to be accepted as comparable, with moderate to high sensitivity and specificity; however, the predictive value of serum ghrelin was somewhat lower. More research is needed to clarify the potential properties of adipokines to gain acceptance as a predictive or diagnostic biomarker for ICP.
Professional Esports Players: Motivation and Physical Activity Levels
Authors: Giakoni-Ramírez Frano , Merellano-Navarro Eugenio , Duclos-Bastías Daniel
Published: February 2022

The professionalisation of esports has increased in recent years, generating the need for further study. Its evolution and continuous development have led the consideration of esports as a profession, increasing the number of players, practice modalities, and hours of play dedicated to this field. The aim of this study was to analyse the relationship between physical activity levels and motivational orientations in an international sample of professional esports players. A cross-sectional and observational study was conducted in European and Latin American countries. The sample was non-probabilistic by convenience, and 260 male professional esports players were recruited. A survey was used that included demographic data, body composition, physical activity (International Physical Activity Questionnaire), and motivation (Sport Motivation Scale). The results show that 92.7% of professional esports players have moderate and high levels of physical activity and that players with low levels of physical activity have positive values in all dimensions of motivation. It is concluded that extrinsic and intrinsic motivation correlates inversely with energy expenditure.
Vestibular Disorders after Kidney Transplantation: Focus on the Pathophysiological Mechanisms Underlying the Vertical Nystagmus Associated with Tacrolimus-Related Hypomagnesamia
Authors: Viola Pasquale , Marcelli Vincenzo , Sculco Domenico , Pisani Davide , Caglioti Alfredo , Ricciardiello Filippo , Scarpa Alfonso , Astorina Alessia , Tortoriello Giuseppe , Gallelli Luca , De Sarro Giovambattista , Chiarella Giuseppe
Published: February 2022

The purpose of this paper is to present the case of a patient undergoing kidney transplantation who developed limb tremor dizziness and vertical nystagmus (ny) during Tacrolimus (TAC) therapy and to investigate the pathophysiological mechanisms underlying the balance disorder. This case study regards a 51-year old kidney transplant male patient with hand tremors and lower limbs asthenia associated with dizziness and nausea. The symptoms started two months after the beginning of intravenous TAC for renal transplantation. The pure-tone audiometry showed a mild symmetrical high-frequencies down-sloping sensorineural hearing loss. Acoustic emittance measures showed a normal tympanogram; stapedial reflexes were normally elicited. The Auditory Brainstem Responses (ABR) and Cervical Vestibular Evoked Myogenic Potentials (c-VEMPs) were bilaterally normally evoked. The bedside vestibular examination showed spontaneous down-beating stationary persistent, omni-positional nystagmus, not inhibited by fixation. The Head-Shaking Test accentuates the spontaneous ny. The horizontal clinical head impulse test was negative, bilaterally. A biochemical blood test revealed a decrease in Magnesium (Mg) levels (0.8 mg/dL; normal range 1.58–2.55). The integration of Mg induced both a plasma levels normalization and an improvement of clinical symptoms. This case suggests that TAC treatment can induce a Mg depletion that caused the transient cerebellar lesion. Therefore, the monitoring of serum electrolytes during immunosuppressive treatment appears to be a useful tool in order to reduce the central system symptomatology.
Hexokinase-2-Linked Glycolytic Overload and Unscheduled Glycolysis—Driver of Insulin Resistance and Development of Vascular Complications of Diabetes
Authors: Rabbani Naila , Xue Mingzhan , Thornalley Paul J.
Published: February 2022

The recent discovery of the glucose-induced stabilization of hexokinase-2 (HK2) to proteolysis in cell dysfunction in model hyperglycemia has revealed a likely key initiating factor contributing to the development of insulin resistance and vascular complications in diabetes. Consequently, the increased flux of glucose metabolism without a change in the expression and activity of glycolytic enzymes produces a wave of increased glycolytic intermediates driving mitochondrial dysfunction and increased reactive oxygen species (ROS) formation, the activation of hexosamine and protein kinase C pathways, the increased formation of methylglyoxal-producing dicarbonyl stress, and the activation of the unfolded protein response. This is called HK2-linked glycolytic overload and unscheduled glycolysis. The conditions required to sustain this are GLUT1 and/or GLUT3 glucose uptake and the expression of HK2. A metabolic biomarker of its occurrence is the abnormally increased deposition of glycogen, which is produced by metabolic channeling when HK2 becomes detached from mitochondria. These conditions and metabolic consequences are found in the vasculature, kidneys, retina, peripheral nerves, and early-stage embryo development in diabetes and likely sustain the development of diabetic vascular complications and embryopathy. In insulin resistance, HK2-linked unscheduled glycolysis may also be established in skeletal muscle and adipose tissue. This may explain the increased glucose disposal by skeletal uptake in the fasting phase in patients with type 2 diabetes mellitus, compared to healthy controls, and the presence of insulin resistance in patients with type 1 diabetes mellitus. Importantly, glyoxalase 1 inducer—trans-resveratrol and hesperetin in combination (tRES-HESP)—corrected HK2-linked glycolytic overload and unscheduled glycolysis and reversed insulin resistance and improved vascular inflammation in overweight and obese subjects in clinical trial. Further studies are now required to evaluate tRES-HESP for the prevention and reversal of early-stage type 2 diabetes and for the treatment of the vascular complications of diabetes.
Effect of Multiple Sclerosis Cerebrospinal Fluid and Oligodendroglia Cell Line Environment on Human Wharton’s Jelly Mesenchymal Stem Cells Secretome
Authors: Salwierak-Głośna Karolina , Piątek Paweł , Domowicz Małgorzata , Świderek-Matysiak Mariola
Published: February 2022

Multiple sclerosis (MS) is a neurological disorder of autoimmune aetiology. Experimental therapies with the use of mesenchymal stem cells (MSCs) have emerged as a response to the unmet need for new treatment options. The unique immunomodulatory features of stem cells obtained from Wharton’s jelly (WJ-MSCs) make them an interesting research and therapeutic model. Most WJ-MSCs transplants for multiple sclerosis use intrathecal administration. We studied the effect of cerebrospinal fluid (CSF) obtained from MS patients on the secretory activity of WJ-MSCs and broaden this observation with WJ-MSCs interactions with human oligodendroglia cell line (OLs). Analysis of the WJ-MSCs secretory activity with use of Bio-Plex Pro™ Human Cytokine confirmed significant and diverse immunomodulatory potential. Our data reveal rich WJ-MSCs secretome with markedly increased levels of IL-6, IL-8, IP-10 and MCP-1 synthesis and a favourable profile of growth factors. The addition of MS CSF to the WJ-MSCs culture caused depletion of most proteins measured, only IL-12, RANTES and GM-CSF levels were increased. Most cytokines and chemokines decreased their concentrations in WJ-MSCs co-cultured with OLs, only eotaxin and RANTES levels were slightly increased. These results emphasize the spectrum of the immunomodulatory properties of WJ-MSCs and show how those effects can be modulated depending on the transplantation milieu.
Metagenomic Sequencing for Microbial DNA in Human Samples: Emerging Technological Advances
Authors: Shi Yu , Wang Guoping , Lau Harry Cheuk-Hay , Yu Jun
Published: February 2022

Whole genome metagenomic sequencing is a powerful platform enabling the simultaneous identification of all genes from entirely different kingdoms of organisms in a complex sample. This technology has revolutionised multiple areas from microbiome research to clinical diagnoses. However, one of the major challenges of a metagenomic study is the overwhelming non-microbial DNA present in most of the host-derived specimens, which can inundate the microbial signals and reduce the sensitivity of microorganism detection. Various host DNA depletion methods to facilitate metagenomic sequencing have been developed and have received considerable attention in this context. In this review, we present an overview of current host DNA depletion approaches along with explanations of their underlying principles, advantages and disadvantages. We also discuss their applications in laboratory microbiome research and clinical diagnoses and, finally, we envisage the direction of the further perfection of metagenomic sequencing in samples with overabundant host DNA.