bluuebunny/crossref_metadata_2025 huggingface dataset:
Biofortification and Quality of Collard Greens as a Function of Iron Concentration in Nutrient Solution
Authors: Mercês Julia Karoline Rodrigues das , Medelo Maria José Yañez , Cecílio Filho Arthur Bernardes
Published: October 2022

The agronomic biofortification of vegetables is a strategy for increasing the concentration of iron (Fe) in food and, consequently, the intake of this micronutrient by the population. When in deficiency, it can cause anemia—a pathology that affects millions of people. Soil-less cultivation is an important cropping system, but there are no studies on how Fe concentrations in a nutrient solution can promote biofortification and affect the quality of collard greens; this is the aim of the present study. For this purpose, five Fe concentrations (2, 4, 6, 8 and 10 mg L−1) were evaluated. Increasing the Fe concentration in the nutrient solution increased the leaf Fe content and decreased the leaf contents of cationic nutrients, without affecting yield. Photosynthetic pigment contents were positively affected by Fe concentrations, while ascorbic acid decreased. There was efficient biofortification of collard greens leaves from 4 mg L−1 Fe, and a higher nutritional quality of leaves was observed at Fe concentrations of 8 mg L−1.
Seaweed Extract Improves Growth and Productivity of Tomato Plants under Salinity Stress
Authors: Hernández-Herrera Rosalba Mireya , Sánchez-Hernández Carla Vanessa , Palmeros-Suárez Paola Andrea , Ocampo-Alvarez Héctor , Santacruz-Ruvalcaba Fernando , Meza-Canales Iván David , Becerril-Espinosa Amayaly
Published: October 2022

Biostimulants constitute an emerging group of crop management products used to enhance productivity under abiotic stress conditions. The ability of some biostimulant products, such as seaweed extracts (SE), to enhance crop tolerance to salinity stress has been documented. SE contain a series of bioactive compounds and signaling molecules, as well as mineral and organic nutrients, that greatly benefit plants. A greenhouse experiment was conducted in order to evaluate SE-mediated tolerance mechanisms in tomato plants under salinity stress. The experiment was divided into two developmental phases (vegetative and reproductive) and included four treatments: control (plants with neither treatment), SE (plants treated with seaweed extract), NaCl (plants irrigated with 300 mM NaCl), and SE + NaCl (plants treated with seaweed extract and irrigated with 300 mM NaCl). Tomato plants treated with the SE from Padina gymnospora showed an increase in root and shoot length (18 cm and 13 cm), root and shoot area (33 cm2 and 98 cm2), and shoot and root fresh weight (1.0 and 3.8 g) under the control and salinity stress conditions. The decrease in productivity (number of fruits) associated with salinity stress was reduced from 28.7% to only 3.4% in SE-treated plants. The positive effects of SE application also included early flowering and enhanced fruit weight and quality. Our findings suggest that optimized photosynthetic performance and antioxidant defense systems (proline, total phenols, and flavonoids) appear to be major factors modulating SE responses to salinity tolerance in tomato plants with promising agricultural applications.
Suaeda glauca and Suaeda salsa Employ Different Adaptive Strategies to Cope with Saline–Alkali Environments
Authors: Song Xiaoqian , Yang Nan , Su Yuhang , Lu Xueyan , Liu Jia , Liu Yang , Zhang Zhonghua , Tang Zhonghua
Published: October 2022

(1) Background: soil salinization has become a global problem that restricts agricultural production; thus, there is a need to explore the special survival strategies of halophytes in saline–alkali environments. (2) Methods: this study conducted a comparative analysis of the differences in metabolites and mineral elements between two indicator plants (Suaeda glauca and Suaeda salsa) in the study area. (3) Results: S. salsa leaves accumulated more total nitrogen (TN), total organic carbon (TOC), calcium (Ca), sodium (Na) and manganese (Mn). The Na/K analysis showed that S. salsa was more tolerant of saline–alkali environments than S. glauca. Metabolite analysis revealed a significant increase in added sugars in S. salsa compared with S. glauca and a significant accumulation of most organic acids associated with the TCA cycle, which suggests an enhancement in the flow of carbon from glycolysis to the TCA cycle. In addition, the content of phenolic substances, such as phenylpropane compounds and flavonols, also changed in saline–alkali environments, which may have promoted the metabolism of organic acids. (4) Conclusions: during the process of plant adaptation to salinity, the central metabolism of S. glauca was nitrogen metabolism, while that of S. salsa was organic acid metabolism.
Toxic Effects of Tetracycline and Its Removal by the Freshwater Microalga Chlorella pyrenoidosa
Authors: Tang Jun , Yang Jun , Zhao Sirui , Wang Xiaoyu , Xie Zhengxin
Published: October 2022

The prevalence of tetracycline (TC) in aquatic environments has raised increasing concern due to its high ecotoxicology risk. The application of microalgae in the removal of antibiotics is a competitive alternative technology. However, the removal mechanism of TC by microalgae and its correlation with the ecotoxic response of microalgae are still not clear. In this study, the ecotoxicity of TC (0.5–10 mg L−1) and its removal by the freshwater alga Chlorella pyrenoidosa were investigated. The results show that TC has significant inhibitory effects on microalgal growth, photosynthetic pigment, and photosynthetic efficiency, with maximum inhibition rates of 49.68%, 62.54%, and 48.08%, respectively. However, the growth inhibition and photosynthesis impairment caused by TC seems to be reversible, as reflected by the decreased inhibition rates with prolonged exposure time. The simultaneous increases in the activities of superoxide dismutase (9.69–23.53%) and peroxidase (15.15–110.92%) and the contents of glutathione (17.62–64.17%) and malondialdehyde (25.16–35.25%) suggest that TC causes moderate oxidative stress. C. pyrenoidosa exhibits high removal efficiency (91.44–95.14%) for TC after 48 h of exposure with short half-lives of 11.31–13.48 h. Biodegradation (56.86–64.62%) is the primary removal mechanism of TC, accompanied by the abiotic process (24.68–40.97%), bioaccumulation (1.95–10.97%), and bioadsorption (0.09–0.38%). These findings demonstrate the toxicity resistance and high removal capacity of C. pyrenoidosa to TC, highlighting its potential application in the remediation of TC-contaminated water.
24-Epibrassnolide Alleviates the Adverse Effect of Salinity on Rice Grain Yield through Enhanced Antioxidant Enzyme and Improved K+/Na+ Homeostasis
Authors: Chen Yinglong , Ge Jianfei , Liu Yang , Li Rongkai , Zhang Rui , Li Kangrui , Huo Zhongyang , Xu Ke , Wei Huanhe , Dai Qigen
Published: October 2022

Previous researchers have focused on the role of 24-epibrassnolide (EBR) in alleviating stresses in plants, whereas the effect of EBR on rice grain yield formation from the perspective of the whole growth stage remains less concerned. To further confirm the optimal application concentration and application periods of EBR in rice (Oryza sativa L.) under salt stress, a seed germination experiment, a seedling experiment, and a pot experiment were designed and conducted. Results showed that EBR treatment significantly enhanced germination indicators and seedling morphological traits, and the effects varied among different EBR concentrations, which were 0.5 > 1.0 > 0.1 > 0.05 mg L−1. Under 0.5% salt treatments, 0.5 mg L−1 EBR spraying significantly enhanced the seedling height, root length, above-/under-ground fresh weight, and above-/under-ground dry weight by 9.2%, 15.9%, 48.0%, 19.5%, 29.3%, and 12.5%, respectively. The spraying of EBR at different periods enhanced rice yield by 6.7% to 94.4% under salt stress. The relatively higher panicle number (increased by 42.9%) and spikelet number per panicle (increased by 96.1%) were the main reason resulting in higher grain yield under the S+T5 (EBR sprayed at both transplanting and heading stage) treatment. Compared to those under S treatment, catalase (CAT) activity was significantly enhanced by 25.0%, while malondialdehyde (MDA) content was dramatically decreased by 37.3% under the S+T5 treatment. The S+T5 treatment significantly enhanced the K+ content in rice root and leaf and decreased the Na+ content in rice root (by 30.4%), thereby leading to higher K+/Na+ under salt stress. The current study concluded that 0.5 mg L−1 was the optimal concentration of EBR in alleviating the adverse effect of salinity. Spraying (transplanting + heading) of EBR twice displayed the best alleviating effect under salt stress, which was realized through enhanced antioxidant enzyme, higher K+ maintenance in leaves, and lowered absorption of Na+ in rice root.
Soil Chemical Properties Depending on Fertilization and Management in China: A Meta-Analysis
Authors: Jia Shengnan , Yuan Ding , Li Wenwen , He Wei , Raza Sajjad , Kuzyakov Yakov , Zamanian Kazem , Zhao Xiaoning
Published: October 2022

The long-term overuse of fertilizers negatively affects soil chemical properties and health, causing unsustainable agricultural development. Although many studies have focused on the effects of long-term fertilization on soil properties, few comparative and comprehensive studies have been conducted on fertilization management over the past 35 years in China. This meta-analysis (2058 data) evaluated the effects of the fertilizer, climate, crop types, cultivation duration and soil texture on the soil chemical properties of Chinese croplands. NPKM (NPK fertilizers + manure) led to the highest increase in pH (−0.1), soil organic carbon (SOC) (+67%), total nitrogen (TN) (+63%), alkali-hydrolysable nitrogen (AN) (+70%), total phosphorus (TP) (+149%) and available potassium (AK) (+281%) compared to the unfertilized control, while the sole nitrogen fertilizer (N) led to the lowest increase. The SOC (+115%) and TN (+84%) showed the highest increase under the influence of NPKM in an arid region. The increase in the chemical properties was higher in unflooded crops, with the maximum increase in the wheat–maize rotation, compared to rice, under NPKM. The SOC and TN increased faster under the influence of organic fertilizers (manure or straw) compared to mineral fertilization. Fertilizers produced faster effects on the change in the SOC and TN in sandy loam compared to the control. Fertilizers showed the highest and lowest effects on change in pH, organic C to total N ratio (C/N), TP and TK in clay loam with the cultivation duration. NPKM greatly increased the C/N compared to NPK in an arid region by 1.74 times and in wheat by 1.86 times. Reaching the same SOC increase, the lowest TN increase was observed in wheat, and the lowest increase in TP and AK was observed in rice, compared to the other crops. These results suggest that organic fertilizers (manure or straw) play important roles in improving soil fertility and in acidification. NPKM greatly increased the potential for soil C sequestration in wheat and in the arid region. The small increases in TP and TK can increase the SOC in rice and in the humid region. Therefore, considering the crop types and climatic conditions, reduced fertilization and the combination of mineral fertilizers with manure may be the best ways to avoid agricultural soil deterioration and increase soil carbon sequestration.
Profile of Selected Mineral Elements in Tibiotarsal Bone of the White-Tailed Sea Eagle in Its Natural Habitat
Authors: Ruszkowski Jakub J. , Hanć Anetta , Komosa Marcin , Dzierzęcka Małgorzata , Mizera Tadeusz , Gogulski Maciej , Zaworska-Zakrzewska Anita
Published: October 2022

Mineral bone composition (dry matter, bones ash, P, Ca, Zn, Mn, Mg, and Cu) and Pb levels of tibiotarsi of seven White-Tailed Sea Eagles were assessed. Lead intoxication in different bird species including waterfowl and raptors is being studied worldwide. The bones were analyzed for Pb by mass spectrometry with excitation in inductively coupled plasma (ICP-MS Elan DRC II) and for bone composition by Atomic Emission Spectrometer (Agilent 4100 Microwave Plasma). Pb levels ranging from 3.54 µg/g to 74.6 µg/g DM suggest that some of the investigated birds might have been intoxicated by Pb. Results of this analysis were divided into two groups of bones, with bone Pb levels higher and lower than Pb toxicity levels, and mineral bone compositions of both groups were compared. The present study shows the differentiation of bone mineral composition among seven examined White-Tailed Sea Eagles, considered a specific species in raptors. Pb intoxication may not have a major influence on mineral bone composition in raptors. It also suggests that assessing bone composition of raptor bones may help finding the possible cause of their deaths.
Comparative Analysis of the Composition of Fatty Acids and Metabolites between Black Tibetan and Chaka Sheep on the Qinghai—Tibet Plateau
Authors: Guo Tongqing , Wang Xungang , Zhang Qian , Wei Lin , Liu Hongjin , Zhao Na , Hu Linyong , Xu Shixiao
Published: October 2022

The objective of this study was to investigate and compare fatty acids and metabolites in the longissimus dorsi muscle between Black Tibetan and Chaka sheep grazing in a highly saline environment. A total of eight castrated sheep (14 months old) with similar body weights (25 ± 2.2 kg) were selected. The experimental treatments included Black Tibetan (BT) and Chaka sheep (CK) groups, and each group had four replications. The experiment lasted for 20 months. All sheep grazed in a highly saline environment for the whole experimental period and had free access to water. The results showed that the diameter (42.23 vs. 51.46 μm), perimeter (131.78 vs. 166.14 μm), and area of muscle fibers (1328.74 vs. 1998.64 μm2) were smaller in Chaka sheep than in Black Tibetan sheep. The ash content in the longissimus dorsi was lower in Chaka sheep than in Black Tibetan sheep (p = 0.010), and the contents of dry matter (DM), ether extract (EE), and crude protein (CP) in the longissimus dorsi showed no differences (p > 0.05). For fatty acids, the proportions of C10:0, C15:0, and tC18:1 in the longissimus dorsi were higher in Chaka sheep than in Black Tibetan sheep (p < 0.05). However, all other individual fatty acids were similar among treatments, including saturated fatty acids (SFAs), unsaturated fatty acids (UFAs), monounsaturated fatty acids (MUFAs), polyunsaturated fatty acids (PUFAs), and the ratios of n-6 PUFAs to n-3 PUFAs and PUFAs to SFAs (p > 0.05). A total of 65 biomarkers were identified between the two breeds of sheep. Among these metabolites, 40 metabolic biomarkers were upregulated in the CK group compared to the BT group, and 25 metabolites were downregulated. The main metabolites include 30 organic acids, 9 amino acids, 5 peptides, 4 amides, 3 adenosines, 2 amines, and other compounds. Based on KEGG analysis, eight pathways, namely, fatty acid biosynthesis, purine metabolism, the biosynthesis of unsaturated fatty acids, renin secretion, the regulation of lipolysis in adipocytes, neuroactive ligand–receptor interaction, the cGMP-PKG signaling pathway, and the cAMP signaling pathway, were identified as significantly different pathways. According to the results on fatty acids and metabolites, upregulated organic acid and fatty acid biosynthesis increased the meat quality of Chaka sheep.
Luteolin and Chrysin Could Prevent E. coli Lipopolysaccharide-Ochratoxin A Combination-Caused Inflammation and Oxidative Stress in In Vitro Porcine Intestinal Model
Authors: Wohlert Annelie , Palkovicsné Pézsa Nikolett , Móritz Alma Virág , Jerzsele Ákos , Farkas Orsolya , Pászti-Gere Erzsébet
Published: October 2022

Ochratoxin A (OTA) and lipopolysaccharide (LPS) intake can cause gastrointestinal disorders. Polyphenolic chrysin (CHR) and luteolin (LUT) display anti-inflammatory and antioxidant properties. Porcine intestinal epithelial (jejunal) IPEC-J2 cells were treated with OTA (1 µM, 5 µM and 20 µM), E. coli LPS (10 µg/mL), CHR (1 µM) and LUT (8.7 µM) alone and in their combinations. Cell viabilities (MTS assay) and extracellular (EC) hydrogen-peroxide (H2O2) production (Amplex red method) were evaluated. Intracellular (IC) reactive oxygen species (ROS) were assessed using a 2′-7′dichlorodihydrofluorescein diacetate (DCFH-DA) procedure. ELISA assay was used to evaluate IL-6 and IL-8 secretion. OTA decreased cell viabilities (p < 0.001) which could not be alleviated by LUT or CHR (p > 0.05); however, EC H2O2 production was successfully suppressed by LUT in IPEC-J2 cells (p < 0.001). OTA with LPS elevated the IC ROS which was counteracted by CHR and LUT (p < 0.001). IL-6 and IL-8 secretion was elevated by LPS + OTA (p < 0.001) which could be inhibited by LUT (p < 0.01 for IL-6; p < 0.001 for IL-8). Based on our results, CHR and LUT exerted beneficial effects on IC ROS levels and on cytokine secretion (LUT) in vitro; thus, they might be used as dietary and feed supplements to avoid OTA- and LPS-related health risks.
Expression Profiling of Circular RNAs in Early Pregnant Jianghuai Buffaloes
Authors: Liu Qiuchen , Zhang Mengya , Guo Tenglong , Wu Sucheng , Zong Yanfeng , Xu Changzhi , Zhu Zhihua , Zhang Yunhai , Cao Zubing
Published: October 2022

Circular RNA (circRNA) is expressed in cells and tissues of several species. However, the expression of circRNAs in the blood of Jianghuai buffaloes during early pregnancy has not been reported. In this study, we identified the DECs in the blood of Jianghuai buffaloes and annotated the functions of these DECs. The results showed that there were 890 DECs between the pregnant and non-pregnant groups, of which more than 80% were exon-derived circRNAs, including 323 up-regulated circRNAs and 567 down-regulated circRNAs. Enrichment analysis revealed that DECs were mainly enriched in the epidermal growth factor receptor-signaling pathway important for embryonic development and pregnancy maintenance. In addition, most DECs have multiple miRNA targets, suggesting that these DECs have the potential to function as miRNA sponges. In conclusion, several DECs are present between pregnant and non-pregnant Jianghuai buffaloes, and these DECs are associated with embryo implantation and pregnancy establishment.
A Spotlight on the Egyptian Honeybee (Apis mellifera lamarckii)
Authors: El-Seedi Hesham R. , El-Wahed Aida A. Abd , Zhao Chao , Saeed Aamer , Zou Xiaobo , Guo Zhiming , Hegazi Ahmed G. , Shehata Awad A. , El-Seedi Haged H. R. , Algethami Ahmed F. , Al Naggar Yahya , Agamy Neveen F. , Rateb Mostafa E. , Ramadan Mohamed F. A. , Khalifa Shaden A. M. , Wang Kai
Published: October 2022

Egypt has an ongoing long history with beekeeping, which started with the ancient Egyptians making various reliefs and inscriptions of beekeeping on their tombs and temples. The Egyptian honeybee (Apis mellifera lamarckii) is an authentic Egyptian honeybee subspecies utilized in apiculture. A. m. lamarckii is a distinct honeybee subspecies that has a particular body color, size, and high levels of hygienic behavior. Additionally, it has distinctive characteristics; including the presence of the half-queens, an excessive number of swarm cells, high adaptability to climatic conditions, good resistance to specific bee diseases, including the Varro disorder, and continuous breeding during the whole year despite low productivity, using very little propolis, and tending to abscond readily. This review discusses the history of beekeeping in Egypt and its current situation in addition to its morphology, genetic analysis, and distinctive characters, and the defensive behaviors of native A. m. lamarckii subspecies.
Primate Conservation Efforts and Sustainable Development Goals in Ecuador, Combining Research, Education and Capacity Building
Authors: de la Torre Stella , Morelos-Juárez Citlalli
Published: October 2022

Ecuadorian primates are a diverse and ecologically important group that is facing severe conservation problems. We present the experiences and lessons learned from two primate conservation projects in eastern and western Ecuador to foster an in-depth reflection of what could be improved to enhance their contribution to the delivery of the Sustainable Development Goals (SDGs). By combining research, education, and capacity building, both projects aim to empower and engage local communities in initiatives to protect primate habitats. These efforts to enhance local environmental sustainability contribute to SDGs 15 (Life on land), 4 (Quality of education), 2 (Zero hunger), 3 (Good health and well-being), 5 (Gender equality), 10 (Reduced inequalities), 12 (Responsible consumption and production), and 13 (Climate actions). One of our findings is that community involvement in conservation activities is not always directly related to an improvement of the conservation status of primate populations. Therefore, continuous monitoring of primate populations and of other relevant indicators is key to assessing the effectiveness of the interventions. We highlight the importance of sharing information between projects with similar scopes and the need to develop local indicators for a more objective assessment of the contribution of small-scale conservation projects to the delivery of the SDGs.
Using Latent Profile Analysis to Evaluate Preferences for Chronic Wasting Disease Management Options among Different Hunter Types
Authors: Rubino Elena C. , Serenari Christopher
Published: October 2022

Wildlife agencies seek to understand how hunters have and will respond to chronic wasting disease (CWD) management policies because of the vital role hunters play in deer management efforts. As such, dozens of studies have examined the human dimensions of CWD management and policy to assess stakeholder support for management alternatives and reveal what drives support. However, most of these studies have not (1) fully explored the heterogeneity that exists among hunters, and (2) accounted for the tradeoffs that agencies and hunters must make with regard to deer management and potential CWD policy alternatives. To address these deficiencies, we used latent profile analysis to create different typologies of hunters based on a survey of Texas hunters, then analyzed discrete choice experiments investigating the CWD management preferences of these typologies. Across five hunter typologies, we found strong overall support for CWD management, although attitudes towards Texas Parks and Wildlife Department were variable. Preferences for CWD management policies greatly differed between each hunter typology. Wildlife agencies can refer to our findings to better develop hunter-preferred CWD management policies and identify areas of compromise between typologies. Our results also provide agencies with insights regarding how to better communicate about CWD management with different types of hunters.
The Significance of Pets for Vulnerable Older Adults during the COVID-19 Pandemic: An Explorative Qualitative Study
Authors: Reniers Peter W. A. , Leontjevas Ruslan , Declercq Ine J. N. , Enders-Slegers Marie-José , Gerritsen Debby L. , Hediger Karin
Published: October 2022

Older adults receiving long-term care at home (LTCH-clients) were impacted by the COVID-19 pandemic and its countermeasures. Previous research suggests that pets can mitigate some of the pandemic’s impacts for older adults but results are contradictory. Our aim was to investigate experiences of LTCH-clients and the significance of their pets during the pandemic. Accounting for saturation, semi-structured interviews were conducted with five LTCH-clients and four family caregivers of LTCH-clients with dementia. Participants were asked about their experiences with COVID-19 and the significance of LTCH-clients’ pets during the pandemic. Two researchers performed thematic analyses in ATLAS.ti using open coding and an iterative–inductive approach. All participants reported negative experiences as a result of COVID-19 countermeasures. Results suggested that caring for pets provided pet owners with structure, which may have contributed to a sense of stability and continuity. Our outcomes underlined an important role of pets for LTCH-clients both before and during the pandemic.
Stem Cells in Domestic Animals: Applications in Health and Production
Authors: Iacono Eleonora , Merlo Barbara
Published: October 2022

In the last decade, researchers described Mesenchymal Stem/stromal cells (MSCs) as a possible population of cells for cell-based therapies in regenerative medicine, both for humans and animals [...]
Defining “Normal” in Pig Parturition
Authors: Walls Alexandra , Hatze Bianca , Lomax Sabrina , Bathgate Roslyn
Published: October 2022

Animal production industries rely on efficient and successful reproductive outcomes, with pigs being no exception. The process of parturition in pigs (farrowing) can be especially prolonged, due to the large numbers of piglets being born (on average, approximately 13 piglets per litter in Australian conditions). Difficulties in farrowing (dystocia) lead to poor piglet outcomes and health problems in sows, in turn, causing economic loss for producers and welfare concerns for the animals. Despite the importance of this topic and publications in the area stretching back nearly 50 years, there is still no consensus on the prevalence of dystocia in pigs nor on how to identify a pig experiencing the condition. Understanding the process of parturition and the factors that influence its success is a crucial step towards the early identification of sows undergoing dystocia and development of best practices to assist them. This article describes the key factors that contribute to successful farrowing and identifies areas in which more research is required before the parturition process in the pig can be fully understood.
Wild Fish Welfare in UK Commercial Sea Fisheries: Qualitative Analysis of Stakeholder Views
Authors: Garratt John K. , McCulloch Steven P.
Published: October 2022

An estimated 1.5–2.7 billion wild fish are caught by UK commercial sea fishing fleets annually. Most are subjected to severe stressors during capture; stunning is rare and gutting alive is common practice. Fish are recognised in UK law as sentient beings, but commercially caught wild fish are excluded from welfare protections. Animal welfare impacts in wild capture sea fisheries are therefore on a massive scale, with major potential for legislative and market-based reforms. Interviews were conducted with 18 experts working within the fishing and seafood industry, fisheries management, scientific research and animal/fish advocacy organisations. The findings reveal a significant welfare gap between societally acceptable ways to treat sentient animals and the reality of capture fisheries. The participants pointed to harms caused to fish throughout different stages of the capture process caused by combinations of variables from fishing gear and methods to biological, environmental and other factors, noting that all require mitigation. Interviews revealed that the nature of harms may be exacerbated by conservative attitudes towards brutal practices in the industry, driven by profit and efficiency and free from legal restraint. To address the welfare gap, stakeholders favour engagement with the industry to improve understanding of harms and to identify mutually beneficial and shared objectives through alleviating stressors to fish in the capture process. This empirical research is focused on UK wild capture sea fisheries. However, given the dearth of welfare legislation globally, it has significance for fishing nations and the many billions of wild sea fish captured each year around the world.
Active Surveillance for Carbapenem-Resistant Enterobacterales (CRE) Colonization and Clinical Course of CRE Colonization among Hospitalized Patients at a University Hospital in Thailand
Authors: Wangchinda Walaiporn , Thamlikitkul Visanu , Watcharasuwanseree Sureerat , Tangkoskul Teerawit
Published: October 2022

Optimal measures for preventing and controlling carbapenem-resistant Enterobacterales (CRE) depend on their burden. This prospective observational study investigated the prevalence and clinical course of CRE colonization in hospitalized patients at Siriraj Hospital, the largest university hospital in Thailand. Stool/rectal swab samples were collected from the patients upon admission, once weekly during hospitalization and every 1–3 months after discharge, to determine the presence of CRE in the stool. Between 2018 and 2021, a total of 528 patients were included. The prevalence of CRE colonization upon admission was 15.5%, while 28.3% of patients who tested negative for CRE on admission acquired CRE during their hospitalization. CRE colonization upon admission was usually associated with prior healthcare exposure. Among CRE-colonized patients, 4.7% developed a CRE clinical infection, with 60% mortality. No cutoff period that ensured that patients were free of CRE colonization in stool was identified, and isolation precautions should only be ceased if stool tests are negative for CRE. In conclusion, the prevalence of CRE colonization among hospitalized patients at Siriraj Hospital is high. CRE-colonized patients are at risk of developing subsequent CRE infection. To prevent CRE transmission within the hospital, patients at high risk of colonization should undergo CRE screening upon admission.
Antibiofilm Effect of Cinnamaldehyde-Chitosan Nanoparticles against the Biofilm of Staphylococcus aureus
Authors: Xu Jiaman , Lin Quan , Sheng Maokun , Ding Ting , Li Bing , Gao Yan , Tan Yulong
Published: October 2022

Food contamination caused by food-spoilage bacteria and pathogenic bacteria seriously affects public health. Staphylococcus aureus is a typical foodborne pathogen which easily forms biofilm. Once biofilm is formed, it is difficult to remove. The use of nanotechnology for antibiofilm purposes is becoming more widespread because of its ability to increase the bioavailability and biosorption of many drugs. In this work, chitosan nanoparticles (CSNPs) were prepared by the ion–gel method with polyanionic sodium triphosphate (TPP). Cinnamaldehyde (CA) was loaded onto the CSNPs. The particle size, potential, morphology, encapsulation efficiency and in vitro release behavior of cinnamaldehyde–chitosan nanoparticles (CSNP-CAs) were studied, and the activity of CA against S. aureus biofilms was evaluated. The biofilm structure on the silicone surface was investigated by scanning electron microscopy (SEM). Confocal laser scanning microscopy (CLSM) was used to detect live/dead organisms within biofilms. The results showed that CSNP-CAs were dispersed in a circle with an average diameter of 298.1 nm and a zeta potential of +38.73 mV. The encapsulation efficiency of cinnamaldehyde (CA) reached 39.7%. In vitro release studies have shown that CA can be continuously released from the CSNPs. Compared with free drugs, CSNP-CAs have a higher efficacy in removing S. aureus biofilm, and the eradication rate of biofilm can reach 61%. The antibiofilm effects of CSNP-CAs are determined by their antibacterial properties. The minimum inhibitory concentration (MIC) of CA is 1.25 mg/mL; at this concentration the bacterial cell wall ruptures and the permeability of the cell membrane increases, which leads to leakage of the contents. At the same time, we verified that the MIC of CSNP-CAs is 2.5 mg/mL (drug concentration). The synergy between CA and CSNPs demonstrates the combinatorial application of a composite as an efficient novel therapeutic agent against antibiofilm. We can apply it in food preservation and other contexts, providing new ideas for food preservation.
Recent Progress in Phage Therapy to Modulate Multidrug-Resistant Acinetobacter baumannii, including in Human and Poultry
Authors: Zhang Yan , Lin Yuanqing , Galgano Salvatore , Houdijk Jos , Xie Weiquan , Jin Yajie , Lin Jiameng , Song Wuqiang , Fu Yijuan , Li Xiuying , Chui Wenting , Kan Wei , Jia Cai , Hu Guangwei , Li Tao
Published: October 2022

Acinetobacter baumannii is a multidrug-resistant and invasive pathogen associated with the etiopathology of both an increasing number of nosocomial infections and is of relevance to poultry production systems. Multidrug-resistant Acinetobacter baumannii has been reported in connection to severe challenges to clinical treatment, mostly due to an increased rate of resistance to carbapenems. Amid the possible strategies aiming to reduce the insurgence of antimicrobial resistance, phage therapy has gained particular importance for the treatment of bacterial infections. This review summarizes the different phage-therapy approaches currently in use for multiple-drug resistant Acinetobacter baumannii, including single phage therapy, phage cocktails, phage–antibiotic combination therapy, phage-derived enzymes active on Acinetobacter baumannii and some novel technologies based on phage interventions. Although phage therapy represents a potential treatment solution for multidrug-resistant Acinetobacter baumannii, further research is needed to unravel some unanswered questions, especially in regard to its in vivo applications, before possible routine clinical use.
The Issue of Pharmacokinetic-Driven Drug-Drug Interactions of Antibiotics: A Narrative Review
Authors: Cattaneo Dario , Gervasoni Cristina , Corona Alberto
Published: October 2022

Patients in intensive care units (ICU) are at high risk to experience potential drug-drug interactions (pDDIs) because of the complexity of their drug regimens. Such pDDIs may be driven by pharmacokinetic or pharmacodynamic mechanisms with clinically relevant consequences in terms of treatment failure or development of drug-related adverse events. The aim of this paper is to review the pharmacokinetic-driven pDDIs involving antibiotics in ICU adult patients. A MEDLINE Pubmed search for articles published from January 2000 to June 2022 was completed matching the terms “drug-drug interactions” with “pharmacokinetics”, “antibiotics”, and “ICU” or “critically-ill patients”. Moreover, additional studies were identified from the reference list of retrieved articles. Some important pharmacokinetic pDDIs involving antibiotics as victims or perpetrators have been identified, although not specifically in the ICU settings. Remarkably, most of them relate to the older antibiotics whereas novel molecules seem to be associated with a low potential for pDDIs with the exceptions of oritavancin as potential perpetrator, and eravacicline that may be a victim of strong CYP3A inducers. Personalized therapeutic drug regimens by means of available web-based pDDI checkers, eventually combined with therapeutic drug monitoring, when available, have the potential to improve the response of ICU patients to antibiotic therapies.
Clinical Characteristics and Outcome of MDR/XDR Bacterial Infections in a Neuromuscular Semi-Intensive/Sub-Intensive Care Unit
Authors: Karruli Arta , Massa Alessia , Bertolino Lorenzo , Andini Roberto , Sansone Pasquale , Dongiovanni Salvatore , Pace Maria Caterina , Pota Vincenzo , Durante-Mangoni Emanuele
Published: October 2022

(1) Background: The aim of this study was to assess the clinical and microbiological characteristics of multidrug-resistant infections in a neuromuscular semi-intensive/sub-intensive care unit; (2) Methods: Retrospective analysis on data from 18 patients with NMD with proven MDRO/XDRO colonisation/infection from August 2021 to March 2022 was carried out; (3) Results: Ten patients were males (55.6%), with a median age of 54 years, and there were fourteen patients (77.8%) with amyotrophic lateral sclerosis. All patients had at least one invasive device. Ten (55.6%) patients developed MDRO/XDRO infection (with a median time of 24 days) while six (33.3%) were colonised. The Charlson comorbidity index was >2 in both groups but higher in the infected compared with the colonised (4.5 vs. 3). Infected patients were mostly females (seven patients) with a median age of 62 years. The most common pathogens were Acinetobacter baumannii and Pseudomonas aeruginosa, infecting four (28.6%) patients each. Of eighteen infectious episodes, nine were pneumonia (hospital-acquired in seven cases). Colistin was the most commonly active antibiotic while carbapenems were largely inactive. Eradication of infection occurred in seven infectious episodes (38.9%). None of those with infection died; (4) Conclusions: MDRO/XDRO infections are common in patients with neuromuscular diseases, with carbapenem-resistant non-fermenting Gram-negative bacilli prevailing. These infections were numerically associated with the female sex, greater age, and comorbidities. Both eradication and infection-related mortality appeared low. We highlight the importance of infection prevention in this vulnerable population.
Aerobic Exercise Training Reduces Atherogenesis Induced by Low-Sodium Diet in LDL Receptor Knockout Mice
Authors: Bochi Ana Paula Garcia , Ferreira Guilherme da Silva , Del Bianco Vanessa , Pinto Paula Ramos , Rodrigues Letícia Gomes , Trevisani Mayara da Silva , Furukawa Luzia Naoko Shinohara , Bispo Kely Cristina Soares , da Silva Alexandre Alves , Velosa Ana Paula Pereira , Nakandakare Edna Regina , Machado Ubiratan Fabres , Teodoro Walcy Paganelli Rosolia , Passarelli Marisa , Catanozi Sergio
Published: October 2022

This study investigated the efficacy of aerobic exercise training (AET) in the prevention of dyslipidemia, insulin resistance (IR), and atherogenesis induced by severe low-sodium (LS) diet. LDL receptor knockout (LDLR KO) mice were fed a low-sodium (LS) (0.15% NaCl) or normal-sodium (NS; 1.27% NaCl) diet, submitted to AET in a treadmill, 5 times/week, 60 min/day, 15 m/min, for 90 days, or kept sedentary. Blood pressure (BP), plasma total cholesterol (TC) and triglyceride (TG) concentrations, lipoprotein profile, and insulin sensitivity were evaluated at the end of the AET protocol. Lipid infiltration, angiotensin II type 1 receptor (AT1), receptor for advanced glycation end products (RAGE), carboxymethyllysine (CML), and 4-hydroxynonenal (4-HNE) contents as well as gene expression were determined in the brachiocephalic trunk. BP and TC and gene expression were similar among groups. Compared to the NS diet, the LS diet increased vascular lipid infiltration, CML, RAGE, 4-HNE, plasma TG, LDL-cholesterol, and VLDL-TG. Conversely, the LS diet reduced vascular AT1 receptor, insulin sensitivity, HDL-cholesterol, and HDL-TG. AET prevented arterial lipid infiltration; increases in CML, RAGE, and 4-HNE contents; and reduced AT1 levels and improved LS-induced peripheral IR. The current study showed that AET counteracted the deleterious effects of chronic LS diet in an atherogenesis-prone model by ameliorating peripheral IR, lipid infiltration, CML, RAGE, 4-HNE, and AT1 receptor in the intima-media of the brachiocephalic trunk. These events occurred independently of the amelioration of plasma-lipid profile, which was negatively affected by the severe dietary-sodium restriction.
Asymmetric Constrained Control of a Cervical Orthotic Device Based on Barrier Sliding Modes
Authors: Mireles Caridad , Lozano Alejandro , Ballesteros Mariana , Cruz-Ortiz David , Salgado Ivan
Published: October 2022

This work proposes a robust sliding mode controller to enforce the tracking trajectory of a cervical orthotic device subjected to asymmetric box constraints. The convergence analysis employs an asymmetric barrier Lyapunov function (ABLF), whose argument is a restricted sliding surface. The stability analysis demonstrates the finite-time convergence of the states towards the sliding surface and, therefore, the exponential stability of the system trajectories. The controller ensures the fulfillment of the restrictions imposed on the sliding surface and consequently over the states. Numerical simulations exhibit the performance of the proposed controller ensuring restricted movements for flexion and extension of a virtual orthotic cervical device. The restricted movements obey asymmetric constraints according to the therapies proposed by medical specialists.
Valorization on the Antioxidant Potential of Volatile Oils of Lavandula angustifolia Mill., Mentha piperita L. and Foeniculum vulgare L. in the Production of Kefir
Authors: Tița Ovidiu , Constantinescu Maria Adelina , Tița Mihaela Adriana , Opruța Tiberius Ilie , Dabija Adriana , Georgescu Cecilia
Published: October 2022

(1) Background: Natural antioxidants are health products found in many plants and may have a therapeutic effect on various diseases caused by oxidative stress. The purpose of this research is the antioxidant analysis of some kefir samples enriched with volatile oils extracted from three aromatic plants; (2) Methods: The volatile oils were extracted from lavender, fennel and mint. Four samples of kefir were made: kefir enriched with encapsulated lavender volatile oil, kefir enriched with encapsulated mint volatile oil, kefir enriched with encapsulated fennel volatile oil and a control sample without volatile oils. The analysis took place in three periods of storage: on the first day, on the 10th day and the 20th day; (3) Results: The antioxidant activity of kefir samples had decreased during the storage. The kefir sample with fennel and lavender volatile oil had the highest antioxidant activity, while the control sample had the lowest activity; (4) Conclusions: We can conclude that the volatile oils add value to the finished product.
Understanding the Mechanism of How Pulsed Electric Fields Treatment Affects the Digestibility and Characteristics of Starch in Oat Flour
Authors: Duque Sheba Mae M. , Leong Sze Ying , Agyei Dominic , Singh Jaspreet , Larsen Nigel , Sutton Kevin , Oey Indrawati
Published: October 2022

The objective of this study was to evaluate the effect of pulsed electric fields (PEF) on the in vitro starch digestibility properties of oat flour. A wide range of PEF process intensity was investigated (electric field strength between 2.1 and 4.5 kV/cm and specific energy inputs between 52 and 438 kJ/kg using 20 μs square wave bipolar pulse at 100 Hz). The results revealed that PEF applied at a high electric field strength and energy <216 kJ/kg was favourable in slowing down the rate of starch digestibility (by 48%) during in vitro gastrointestinal digestion. This is accompanied by a significant decrease (from 15% to 7–10%) in the proportion of rapidly digestible starch (RDS) and a significant increase (from 77% to 84–85%) in resistant starch (RS) fraction. The application of PEF at energy level >421 kJ/kg at any field strength intensities raised the RDS (from 15% to 19–20%), but the rate of starch digestion was not affected (maintained at 3.3–3.7 × 10−2 min−1 vs. untreated at 3.8 × 10−2 min−1). Further analysis of the structure, particle size, and thermal stability of PEF-treated oat flour through fractionation into three distinct flour segments revealed that PEF could cause major modifications in the particle size, damage and aggregation of starch granules, and destruction of the long- and short-range ordered structures of starch. Data gathered in this study indicate that PEF treatment can be a reliable strategy to modulate the in vitro starch digestibility of oat flour, either by reasonably slowing down the digestion rate or enabling a slightly higher amount of starch to be readily accessible by digestive enzymes without affecting the digestion rate.
Quantum Image Steganography Schemes for Data Hiding: A Survey
Authors: Min-Allah Nasro , Nagy Naya , Aljabri Malak , Alkharraa Mariam , Alqahtani Mashael , Alghamdi Dana , Sabri Razan , Alshaikh Rana
Published: October 2022

Quantum steganography plays a critical role in embedding confidential data into carrier messages using quantum computing schemes. The quantum variant of steganography outperforms its classical counterpart from security, embedding efficiency and capacity, imperceptibility, and time-complexity perspectives. Considerable work has been carried out in the literature focusing on quantum steganography. However, a holistic view of available schemes is missing. This paper provides an overview of latest advances in the field of quantum-steganography and image-steganography schemes. Moreover, the paper includes discussion of improvements made in the aforementioned fields, a brief explanation of the methodologies used for each presented algorithm, and a comparative study of existing schemes.
Comments on the Physics of Microwave-Undulators
Authors: Di Palma Emanuele , Dattoli Giuseppe , Sabchevski Svilen
Published: October 2022

The properties of electromagnetic undulators, produced by the field of a high-power microwave, are discussed. We analyze the conditions to be satisfied that allow the treatment of the emission process by relativistic charges moving through it in full analogy with the case of their static counterparts. We critically review the often claimed possibility of exploiting them in programs aimed at reducing the sizes and costs of an actual free electron laser (FEL) device. We analyze several possible configurations for FELs based on powerful microwave undulators and conclude that the present level of technology, even though not yet mature, should be improved to allow a breakthrough either for low- or high-gain devices.
Analysis of Wideband Scattering from Antenna Based on RFGG-FG-FFT with Cube Polynomial Inter/Extrapolation Method
Authors: Kong Weibin , Zheng Yongtao , Song Yubin , Fang Zhongqing , Yang Xiaofang , Zhang Haonan
Published: October 2022

This paper presents an efficient real-coefficient fitting both Green’s function and its gradient with Fast Fourier Transform (RFGG-FG-FFT) with cube polynomial inter/extrapolation method (CPIE), which is established for the analysis of scattering from antenna over a wide frequency. To improve the computation efficiency, the CPIE is utilized. In order to reduce memory requirements and accelerate matrix vector multiplication, the RFGG-FG-FFT is adopted. The accuracy, correctness and efficiency of the new method are researched on some examples. Compared with the direct method, the examples show that the new method is superior in broadband without loss of accuracy.
Research on Design Method of Product Functional Hybridization for Integrated Innovation
Authors: Liu Fang , Jing Yingchao , Shao Peng , Zhang Yazhen
Published: October 2022

Product hybridization design is a new model of integrated innovation. Existing methods of product hybridization design focus on technological recombination, and there is a lack of research from the perspective of function. Therefore, this paper proposes the concept of functional product hybridization. Obtaining goal products and fusing the existing product function systems are two keys to implementing product functional hybridization. However, existing functional integration methods acquire goal products too widely, and there is less research on fusing product function systems. In this paper, a scenario analysis model based on the divergence tree is established by combining scenario analysis and the divergence tree, and three paths of goal product prediction for functional hybridization are proposed. Based on the idea of biological gene recombination, a product gene model and a method of product gene recombination for functional hybridization are studied. Moreover, integrating the Theory of Inventive Problems Solving (TRIZ), a method of establishing a concept structure is proposed. On this basis, a process model for product functional hybridization design is established. An example of a new tree-planting machine illustrates the application of the proposed model. The proposed method enriches the theory of product hybridization design and achieves the fusion of product function systems to meet the multi-functional needs of users.