bluuebunny/crossref_metadata_2025 huggingface dataset:
Enzyme activation and inhibition induced by cold provocation in a patient with cold urticaria
Authors: Martens PM , Berrens L.
Published: July 2023

A 33-year-old female patient with acquired cold urticaria, together with her 8-year-old healthy daughter, was subjected to a brief period of cold exposure. The effect of this treatment upon a number of key factors of the plasma coagulation, kallikrein and complement systems was investigated. Cold air provocation caused increased fibrinolysis, together with a measurable consumption of the protease inhibitors alpha1-antitrypsin (alpha1AT), alpha2-macroglobulin (alpha2M) and C1Inactivator (C1INA). Kaolin activation of the patient's plasma elaborated exceptionally high levels of esterolytic activity, both before and after cold exposure, indicating pre-enzyme lability. Both subjects had abnormally high serum ratios alpha2M/alpha AT. Impressive leucocytosis was observed in the symptomless child.
Lipo-atrophy of the ankles and its relation to other lipo-atrophies
Authors: Jablonska S , Szczepanski A , Gorkiewicz A.
Published: July 2023

Two typical cases of lipo-atrophy of the ankles are described, one case of atrophy of the ankles with associated atrophy of the whole extremity, and one case of localized atrophy of the knee. For comparison, we present one case of atrophy in a diabetic patient due to insulin injections but developing at distant sites, and in one case, a child in whom atrophy followed antibiotic injections. Primary inflammatory vascular changes in the subcutaneous tissue were demonstrated in atrophy of the ankles, as in the early period of insulin-induced lipo-atrophy. Attention is called to the possible relation of the described lipo-atrophies.
Studies on topical antiperspirant control of axillary hyperhidrosis
Authors: Shelley WB , Hurley Jr HJ.
Published: July 2023

Axillary hyperhidrotics is reviewed from the standpoint of anatomical factors, physiological mechanisms and the history of methods of control. Anhydrous aluminum chloride and anhydrous zirconium tetrachloride are shown to be superior topical agents for partial control of axillary sweating when applied as a powder or in anhydrous nonreactive vehicles. Complete anhidrosis as demonstrated by sustained garment armpit dryness could be achieved in hyperhidrotics within 48 hours by the following trinary antiperspirant system: (1) a saturated solution of aluminum chloride hexahydrate or zirconyl chloride in absolute ethanol or isopropyl alcohol, (2) application to the dry axilla at times of sleep or other prolonged non-sweating period, (3) water vapor occlusion of area for 6 to 8 hours by means of Saran wrap. The hypothesis is presented that metallic antiperspirants act by reflux entrance into the terminal intraepidermal eccrine duct, slowly combining with the intraductal keratin, to produce a fibrillar contraction (super contraction) of keratin and hence functional closure, not histologically evident. This altered keratin is shed weeks later, with the consequent return of ductal patency and sweating.
Use of affinity chromatography for purification of tyrosinase
Authors: Menon IA , Haberman HF.
Published: July 2023

The possibility of using affinity chromatography for the purification of tyrosinase was explored. When purified mushroom tyrosinase was employed, almost all tyrosinase became bound to Sepharose containing 3-iodotyrosine, an inhibitor of tyrosinase. When crude extracts from B16 melanoma were employed, partial purification of tyrosinase and separation of two or possibly three forms of tyrosinase could be obtained. The results indicate that affinity chromatography could possibly be employed for the purification of tyrosinase during the final stages of the purification procedures.
On the diagnosis of Fabry's disease
Authors: Wadskov S , Andersen V , Kobayasi T , Søondergaard J , Søorensen SA.
Published: July 2023

Fabry's disease is a recessive X-linked inborn error of metabolism due to deficiency of the lysosomal enzyme alpha-galactosidase. The large variety of symptoms may make the diagnosis difficult. A severely afflicted female patient is presented. For several years she had been treated under the diagnosis polyarteritis nodosa until the characteristic cutaneous lesions of Fabry's disease were recognized. Enzymatic studies and electronmicroscopic examinations confirmed the diagnosis. A symptomatic effect of corticosteroid treatment was proven. The grave prognosis, the recent attempts at enzyme substitution therapy and the possibility of preventing new cases by prenatal diagnosis should stimulate the efforts of the clinician to diagnose the disease.
Herpes simplex: cicatricial type
Authors: Shelley WB.
Published: July 2023

An example of a cicatricial form of recurrent herpes simplex is presented. Although the initial appearance of the lesions was identical with non-scarring forms of herpes simplex, this patient's lesions progressed insidiously to eschar formation. Healing was delayed for four to seven weeks, with subsequent atrophic scar formation. Scarring and local recurrence could be prevented by curettage, and acridine orange and ultraviolet light therapy within 48 hours of an attack. Other modalities employed over a fourteen year period were largely ineffectual. It was concluded that the ulceration and scar formation were an intrinsic part of the herpetic process in this patient.
Pseudoxanthoma elasticum. An ultrastructural study of scar tissue
Authors: Danielsen L , Kobayasi T.
Published: July 2023

Abstract is not availeble.
X-ray fluorescence analysis of skin in pseudoxanthoma elasticum
Authors: Blumenkrantz N , Danielsen L , Jorgensen K.
Published: July 2023

Abstract is not availeble.
Skin and nail disorders in relation to chronic renal failure
Authors: Kint A , Bussels L , Fernandes M , Ringoir S.
Published: July 2023

Abstract is not availeble.
Studies on guinea pig skin cell cultures. IV. Collagen synthesis
Authors: Frey J , Delescluse C , Biron A , Farjanel J , Prunieras M.
Published: July 2023

Abstract is not availeble.
Effects of long term treatment with tetracycline
Authors: Delaney TJ , Leppard BJ , MacDonald DM.
Published: July 2023

Abstract is not availeble.
Ultrastructural changes in scleromyxedema
Authors: Danielsen L , Kobayasi T.
Published: July 2023

Skin biopsy specimens from a 60-year-old patient with paraproteinemia and generalized changes of the skin typical of scleromyxedema were studied with the electron microscope. The dermis was dominated by collagen fibrils and accumulations of peculiar connective tissue cells, while elastic tissue was sparse and in some areas completely absent. Large, sharply demarcated areas of a filamentous material were occasionally observed. The collagen fibrils were often surrounded by thin filaments with a periodic segmentation and by many glycosaminoglycan (mucopolysaccharide) filaments. The elastic fibres contained large amounts of elastic fibrils and small amounts of a homogenous matrix. The cytoplasm of the above-mentioned cells was dominated by lysosomes in different stages of development, often occupying almost all the cytoplasmic area. The collagen fibrils were found in close relation to these cells, frequently inside invaginations of the cells. Furthermore, collagen fibrils were observed free in the cytoplasmic area and inside the lysosomes, indicating lysosomal degradation of collagen.
Erythema dyschromicum perstans (ashy dermatosis). Report of two cases from Scandinavia
Authors: Holst R , Mobacken H.
Published: July 2023

Abstract is not availeble.
Acanthoma fissuratum--spectacle frame acanthoma
Authors: MacDonald DM , Martin SJ.
Published: July 2023

The development of a cutaneous nodule at the site of friction of spectacles has been discussed by several authors. In all but one case the lesion occurred behind the ears. The only previous report of this acquired tumour occurring on the nose is that of Farrell & Wilson (4). It is suggested that this problem is relatively common and most frequently presents when mistaken for basal cell epithelioma or when identified in medical personnel. A further six cases of this neglected tumour occurring on the nose are described.
Plasma cell granulomas in non-lipemic xanthomatosis: apparent induction by indomethacin
Authors: Shelley WB , Rawnsley HM.
Published: July 2023

A 71-year-old man with non-lipemic, generalized, plane xanthomas developed nodules and tumors within one month of starting indomethacin therapy. These enlarged and spread during 5 months of daily indomethacin but promptly and spontaneously involuted when the indomethacin was discontinued. Within 4 months, the nodules and tumors were completely gone. The process is viewed as an "indoderma", i.e. a granulomatous drug reaction, analogous to an iododerma.
Patch testing, tuberculin testing and sensitization with dinitrochlorobenzene and nitrosodimethylanilini of patients with atopic dermatitis
Authors: Forsbeck M , Hovmark A , Skog E.
Published: July 2023

Cell-mediated immunity was studied in patients with atopic dermatitis. 113 patients were patch tested with ten contact allergens. The frequency of positive reactions to patch testing with "common contact allergens" was found to be lower in patients with "high IgE values" than in those with IgE less than or equal to 1000 U/ml. A larger number of patients with severe dermatitis reacted negatively to PPD and were more difficult to sensitize with DNCB and NDMA as compared with the patients with mild dermatitis. The results of this investigation support the findings of earlier workers that patients with atopic dermatitis show disturbances in the cell-mediated immune system and these disturbances appear to be correlated to the degree of severity of the dermatitis.
Hereditary zinc deficiency (Adema disease) in cattle, an animal parallel to acrodermatitis enteropathica
Authors: Weismann K , Flagstad T.
Published: July 2023

Adema disease and acrodermatitis enteropathica, two parallel syndromes in calves of Friesian descent and in man, are described. Both are congenital zinc deficiency disorders with a lethal course if left untreated. Complete recovery follows oral zinc therapy. Symptoms and findings are set out in Table I. Diseased calves may serve as animal models for further studies on acrodermatitis enteropathica and the biological role of zinc.
Thymidine labelling of epidermal melanocytes in UV-irradiated skin
Authors: Rosdahl IK , Szabo G.
Published: July 2023

By using autoradiography and a technique of repeated 3H-thymidine injections, it was found that there is a normal turnover of epidermal melanocytes in C57BL mice, and that the numerical increase in the population of active melanocytes following repeated UV irradiation is essentially the result of cell division.
Apoptosis in lichen planus and several other dermatoses. Intra-epidermal cell death with filamentous degeneration
Authors: Hashimoto K.
Published: July 2023

In hyperplastic conditions of the skin, eosinophilic bodies are often observed in the epidermis and upper dermis. These have been named variously Civatte body of lichen planus and dyskeratotic cell of actinic keratosis. In the present studies, it was found that (i) these cells commonly contain whorls of distinct filaments (60-80 A), which may be attached to desmosomes; (ii) unlike keratinized cells, the cellular envelope was not thickened by the formation of the marginal band; (iii) the cytoplasm contains a large number of vacuoles; (iv) the nucleus is lost by condensation or diffuse disintegration; and (v) these cells could be dropped into the upper dermis and filamentous contents could be released to dermal phagocytes. From these observations it was concluded that Civatte bodies and other eosinophilic bodies of neoplastic and hyperplastic epidermis represent "filamentous degeneration" or premature keratinization of keratinocytes.
Dissociation of human adult epidermal cells by disulfide-reducing agents and subsequent trypsinization
Authors: Hentzer B , Kobayasi T.
Published: July 2023

Primary cultures of epidermal cells from human adult skin were dissociated by dithiothreitol (DTT) with subsequent trypsinization. After treatment with disulfide reducing agents, the desmosomes were either destroyed or showed a homogeneous ultrastructure. Trypsin separated the homogeneous desmosomes, whereas cell membranes, organelles, and nuclei remained undamaged. Trypsin did not influence normal desmosomes. A suspension of human epidermal cells was recultivated in new flasks. Two types of cells grew in the cultures--polygonal and spindle-shaped. The polygonal cells were maintained for 30 to 60 days and grew slowly. The spindle-shaped cells were leucine-amino-peptidase negative, grew actively, and were subcultured.
Effects of dibutyryl adenosine 3',5'-cyclic monophosphate on human melanocytes in vitro
Authors: Kitano Y.
Published: July 2023

The effects of cyclic AMP and its analogue, dibutyryl cyclic AMP (DBcAMP) on the pigmentary system were studied by using human epidermal melanocytes in culture. The melanocytes responded to 1 mM DBcAMP with an increase in number, length, and complexity of dendritic processes. The effect of DBcAMP on the dendritogenesis was reversible. Melanin synthesis, as indicated by the uptake of tyrosine in the presence of an inhibitor of protein synthesis, was significantly stimulated by DBcAMP. The maximum stimulation was observed at concentrations of 0.5 mM, and 1.0 mM. The melanin synthesis increased after 12-hour treatment with DBcAMP and continued to increase with the prolonged treatment. Cyclic AMP, theophylline, sodium butyrate, or 5'-AMP at a concentration of 1 mM did not have any remarkable effect on the morphology or the melanin synthesis of the melanocyte. The results of this investigation indicate the possible role of the MSH-cyclic AMP system in the melanin pigmentation of human skin and represent a system for further study of the pathobiology of human melanocytes.
Study of some minerals in scalp hair and blood sera of psoriatics
Authors: Abdel-Aal H , Soliman AA , El Mahdy H , El  Saiee L.
Published: July 2023

Scalp hair and blood sera of two groups of psoriatic individuals were examined regarding some of their mineral contents by flame photometric and colorimetric procedures, and the findings compared with a normal control group. The result obtained revealed changes in the mineral content of scalp hair of psoriatics. A possible relationship may exist between hair and serum minerals.
The leukocyte migration test in nickel contact dermatitis
Authors: Thulin H.
Published: July 2023

Ten patients with contact dermatitis to nickel, confirmed by patch testing, were investigated by the leukocyte migration test. Specific inhibition of leukocyte migration was obtained with nickel protein complexes. Bovine albumin, human albumin and human epidermis protein were used as carrier proteins in the complexes. Specific inhibition of leukocytes from patients with positive patch test to nickel could not be demonstrated with the hapten alone.
Erythema nodosum and erythema multiforme associated with milker's nodules
Authors: Kuokkanen K , Launis J , Mörttinen A.
Published: July 2023

An epidemic of 44 cases of milker's nodules was recorded in the Tampere Central Hospital catchment area in Finland during the autumn of 1974. Exanthema or erythema multiforme-like secondary eruptions were seen in 10 cases. One female patient is reported in detail because of the simultaneous occurrence of erythema nodosum and erythema multiforme. The virological diagnosis was confirmed by electron microscopy.
Suppressed cell-mediated immunity associated with eczematous inflammation
Authors: Uehara M , Ofuji S.
Published: July 2023

In order to determine if an eczematous inflammation could have any influence upon already established cell-mediated immune reactivity, a widespread allergic as well as primary irritant, eczematous dermatitis was produced in tuberculin-sensitive guinea pigs. Their tuberculin reactivity was significantly suppressed both by allergic contact dermatitis due to dinitrochlorobenzene and by primary irritant dermatitis due to croton oil. After the dermatitis had subsided, tuberculin reactivity was restored to approximately the same level as before the induction of the dermatitis, which suggests that the suppression is temporary.
Malignant acanthosis nigricans--a para-endocrine syndrome?
Authors: Hage E , Hage J.
Published: July 2023

A case is reported of acanthosis nigricans associated with a gastric carcinoma. The gastric cancer was of the diffuse, infiltrating type. Many tumour cells were identified by means of histochemistry and electron microscopy as neoplastic enterochromaffin-like cells. It is suggested that cancers associated with acanthosis nigricans might constitute a certain group of carcinomas in which parts of the tumour cells arise from the APUD-series of endocrine cells.
The histocompatibility antigens in patients with Behçet's disease
Authors: Haim S , Gideoni O , Barzilai A.
Published: July 2023

Twenty-one patients with Beh√ßet's disease were typed for HLA antigens. The controls were 50 subjects randomly chosen from a local population and 24 apparently healthy subjects of the same ethnic groupings as those of the patients. The study revealed significant differences in the frequencies of various antigens in the above three groups, the most obvious of them related to HLA-B5 and HLA-Bw35, HLA-B5 was demonstrated in 71% of the patients as compared with 18% in the local population group and 12.5% in the ethnically related healthy subjects. The incidences of HLA-Bw35 were 66.7%, 32% and 37.5% respectively. In 86% of the patients, at least one of these two antigens was present. It is suggested that besides contributing to the understanding of the disease process, the determining of associated specific antigens may constitute a further diagnostic acid in doubtful cases of Beh√ßet's disease.
The treatment of balanitis xerotica obliterans with testosterone propionate ointment
Authors: Pasieczny TA.
Published: July 2023

Balanitis xerotica obliterans (BXO) and kraurosis penis are thought to be synonymous. Clinically and histopathologically they probably represent the same disease process as lichen sclerosus et atrophicus (LSA) but also involve the urethral mucosa. The treatment of choice is considered to be 2 1/2% testosterone propionate ointment which gives better results than strong corticosteroid applications, yet without their side effects.
Phenothiazine phototoxicity: an experimental study on chlorpromazine and related tricyclic drugs
Authors: Ljunggren B , Möller H.
Published: July 2023

A large number of phenothiazines and chemically related tricyclic drugs have been studied with respect to their phototoxic potency. Two methods were used, an in vivo technique based on the inflammatory response of the mouse tail after systemic administration of the drug plus UVA irradiation, and an in vitro method based on growth inhibition of Candida albicans. Of 27 commercial tricyclic drugs tested in vivo, the most potent were chlorpromazine and two other chlorinated compounds, prochlorperazine and perphenazine. Tricyclic drugs lacking nitrogen, sulphur, or both in their ring system showed no activity. All compounds phototoxic in the mouse were so in the yeast test as well. Here, however, the thioxanthenes (lacking nitrogen) were also highly active.
Squamous cell carcinoma appearing in X-ray-treated mycosis fungoides
Authors: Volden G , Larsen TE.
Published: July 2023

Two patients with mycosis fungoides developed a squamous cell carcinoma of the skin on the neck. Upon verification by routine biopsy testing of material taken from a suppurating infiltrate of the neck, both patients were treated intermittently with X-rays. Both had developed the squamous cell carcinoma on a sun-exposed area. Patient 1 had been treated with Grenz-ray irradiation totalling 10 kilovolt (kV) 4400 rad. and Dermopan step IV (50 kV) 5200 rad.; while patient 2 recieved altogether: Grenz-rays 4600 rad., Dermopan step IV 400 rad., and soft X-rays (150 kV) 800 r. The latency period was, respectively, 3 and 10 years.
Chronic dental fistule on the nose
Authors: Tagami H , Yoshitake K.
Published: July 2023

A case of chronic dental draining sinus on the dorsum of the nose is reported. The skin lesion, clinically simulating basal cell epithelioma, healed promptly after extraction and eradication of the periapical abscess of the right upper canine. The importance of considering this condition is emphasized in cases of chronic facial granulomatous or ulcerative lesions.
Unusual normolipidemic cutaneous xanthomatosis: a comparison of two cases illustrating the differential diagnosis
Authors: Hu CH , Winkelmann RK.
Published: July 2023

A patient who developed diffuse normolipidemic plane xanthomas also presented with IgG lambda monoclonal gammopathy, hypernephroma, an unusual family cluster of leukemia (with two family members in two generations), and a unique, acquired C1-esterase inhibitor deficiency. A second patient presented with widespread normolipidemic papular xanthomas in which histiocytes containing Langerhans' granules were found. The lipid composition of the lesions of these two patients showed striking differences. Excesses of triglycerides and cholesterol ester were demonstated in plane xanthoma, whereas phospholipids were prominent in the popular xanthoma of histiocytosis X. We present and classify these two cases to emphasize the diagnostic value of chemical and ultrastructural studies of normolipidemic cutaneous xanthomatosis.
Elastosis perforans serpiginosa with widespread arterial lesions: a case report
Authors: Eide J.
Published: July 2023

A case is presented of elastosis perforans serpiginosa (EPS) with unilateral dermal lesions, widespread arterial lesions with aortic rupture, and elastosis of the endocardium and bronchiolar walls. Other chronic skin disorders with lesions resembling EPS are discussed; and the arterial lesions compared with some arterial diseases. The findings support a concept of the disease as a focal affection of elastic tissue, not only in the skin, but also in arteries and other organs.
Kaposi's sarcoma in polar eskimos
Authors: Mikkelsen F , Nielsen NH , Hansen JP.
Published: July 2023

Two cases of Kaposi's sarcoma (KS) are described in patients of pure Eskimo trait from the northernmost part of the world. The clinical and histological findings corresponded to KS in other parts of the world. This report on KS in Eskimos from the sparsely populated Arctic areas may support the opinion that KS is more common outside Africa than the literature indicates.
Ultrastructural changes in disseminated connective tissue nevi
Authors: Danielsen L , Kobayasi T , Jacobsen GK.
Published: July 2023

Skin biopsy specimens from a patient with disseminated connective tissue nevi and from her son and daughter who had no such clinical alterations were studied with the electron microscope. The nevi showed increased amounts of thick elastic fibres, which were composed of irregularly arranged, often finger-like, subunits surrounded by a thready material. Both structures probably represent malformed components of elastic fibres, the thready material representing malformed elastic fibrils. Many of the collagen fibrils showed increased diameters and twistings, which alterations are believed to represent malformation of collagen. The fibroblasts showed changes which might be related to a pathological protein synthesis. Examination of biopsy specimens from normal skin of the patient and from the skin of her son and daughter failed to demonstrate similar alterations. The present case is regarded as an entity, distinct from dermatofibrosis lenticularis disseminata and from shagreen patch.
Transcutaneous nerve stimulation and itching
Authors: Fjellner B , Hägermark O
Published: July 2023

The response to transcutaneous nerve stimulation (TNS)--a method used for treatment of chronic pain--was studied in 41 patients with itching of diverse etiology. At a first trial, 63% of the patients found that TNS ameliorated their itching, 20% reported complete relief. As a rule the effect lasted for many hours, although TNS was given only for 5-30 min. In 15 of the patients, having suffered from extensive pruritus for more than one year, TNS was given several times a day for 5-47 days. During this time the effect declined. Twelve patients were relieved initially, either partially or completely, but ultimately only 6 had a partial relief and in none had the itching disappeared completely. Only one patient wanted to continue the TNS therapy. The decreasing efficacy is discussed; probably there was an initial placebo effect which declined during the course of treatment. The results indicate that TNS is of limited value for treating chronic itching.
Keratinosomes in psoriatic skin
Authors: Mottaz JH , Zelickson AS.
Published: July 2023

Keratinosomes in psoriatic skin can be slightly larger than those in normal skin. Some seem to develop an unusual internal morphology, and some reach their full development while they are still within the immediate Golgi area. Observational evidence indicates that there may be more kerantinosomes in psoriatic skin than are generally found in normal skin. Keratinosomes appear extracellularly from the spinous layer through the horny layer. Some keratinosomes do not move to the outside of the cell and can be seen within cells of the horny layer. There appears to be an increased number of keratinosomes in and between the outer cells of sweat ducts. Keratinosomes are also present within the dark cells of the duct and the extracellular space between the dark cells contains material from keratinosomes.
Studies on carrier protein in contact dermatitis: localization of soluble epidermal carrier proteins by macrophage migration test
Authors: Miyagawa N , Miyagawa S , Ishii H , Hayashi K , Sakamoto K , Aoki T
Published: July 2023

The migration of peritoneal exudate cells from picrylchloride-sensitive guinea pigs was inhibited by heteroantigenic components which were isolated by antinormal guinea pig epidermis antibody immunoadsorbent from soluble epidermal proteins of picrylchloride painted guinea pigs. The components were demonstrated by indirect immunofluorescence study to localize in the intercellular spaces of guinea pig epidermis. It is consequently suggested that carrier protein(s) of contact hypersensitivity reside on the cell surface of epidermis.
Local excision in the treatment of oral discoid lupus erythematosus
Authors: Schiödt M
Published: July 2023

Oral lesions in eight patients with discoid lupus erythematosus (DLE) were treated with local excision. Ten well-demarcated oral lesions were excised and a primary closure was obtained following the excision. Six patients out of seven became symptom-free following the treatment. The median observation time was 1.0 year. The follow-up examination showed erythema in one case and recurrence in one case at the operation site. In the remaining eight operation sites, a soft scar with no sign of recurrence was seen. These results indicate that the method should be investigated further in order to obtain long-term results. Local excision of oral discoid lesions may be a valuable therapeutic supplement in some cases of DLE.
Cellular aspects of phototoxic reactions induced by kynurenic acid II Fine structural analysis and cytochemistry on cultivated cells
Authors: Wennersten G , Brunk U
Published: July 2023

A recent report described the establishment of an experimental model for studying photo-oxidative damage inflicted by kynurenic acid on cultivated cells. Preliminary findings by scanning electron microscopy (SEM) were reported. For the present paper supplementary SEM studies were performed, and it was found that maximum cell damage appears 2 to 4 hours post irradiation. With low concentrations of kynurenic acid and brief UVA exposure, repair mechanisms seem to occur. Damage in the form of cellular swelling and plasma membrane alterations is most easily recognized by SEM, which is far more sensitive than ordinary light microscopy. Transmission electron microscopy (TEM) reveals morphologic alterations, consisting of an enhanced formation of endocytotic vacuoles from the plasma membranes, swelling with disruptions of the cell sap continuity, mitochondrial swelling, dilatation of the endoplasmic net, and vesiculation of some of the microvilli. Lysosomal damage, which was not seen until very late post irradiation and only for high concentrations of kynurenic acid and long-term UVA exposure, seems to be a secondary phenomenon. This hypothesis is further supported by the cytochemical analysis. The primary event thus seems to affect the plasma membrane level and originate in the binding of kynurenic acid to the plasma membrane on which subsequent UVA irradiation inflicts secondary intracellular alterations due to increased permeability and swelling.
Autoradiographic investigation on benzoyl peroxide treated skin
Authors: Fanta D , Jurecka W
Published: July 2023

In vitro autoradiography was used to study the effect of a 5% benzoyl peroxide preparation, or its gel base alone. After three weeks' local application, there was no change in the labelling index of epidermis or follicular infundibulum, while there was a significant reduction in the labelling index of the sebaceous glands. Treatment with the base alone brought about no change.
Bone cysts and spontaneous fractures in two siblings with dyskeratosis follicularis Darier
Authors: Menné T , Nielsen AO
Published: July 2023

Two siblings with Darier's disease, multiple bone cysts and recurrent "spontaneous" fractures are described.
Quantification of anaerobic diphtheroids on the skin
Authors: Aly R , Maibach HI , Bloom E
Published: July 2023

The widely used technique for sampling skin bacteria employs a detergent (Triton X-100) in buffer solution to remove and suspend the microorganisms. The fraction of the total population of anaerobes on the forehead removed by scrubbing with this solution was estimated. We calculated that approximately 10% of the resident anaerobes were removed by one minute of scrubbing with the detergent solution and two 1-min washes remove approximately 19.5% of the resident anaerobes (8.8 X 10(5)/CM2).
Juvenile plantar dermatosis
Authors: Neering H , Van Dijk E
Published: July 2023

Juvenile plantar dermatosis seems to be a new skin disease found mainly in
Tricho-rhino-phalangeal syndrome in five successive generations: Report on a family in Finland
Authors: Peltola J , Kuokkanen K
Published: July 2023

A family of 11 members with tricho-rhino-phalangeal syndrome (TRP syndrome) is described. The inheritance in five successive generations was autosomal dominant. The main features of the TRP syndrome noted were: sparse hair, pear-shaped nose and joint deformity with cone-shaped epiphyses at some of the middle phalanges of the hands.
Failure to detect circulating IgG or IgM antibodies to basal cell carcinoma by immunofluorescence
Authors: Blewitt RW , Aparicio SR , Burrow HM , Rowell NR , Rowell RM
Published: July 2023

Immunofluorescence techniques failed to reveal evidence of anti-tumour antibody in the sera of patients with basal cell carcinima. Although the presence of such antibodies has previously been associated with the absence of metastasis in malignant melanoma, other explanations for the low metaststic potential of basal cell carcinoma should be sought.
Penetration of 65Zn through the skin of rats
Authors: Hallmans G , Lidén S.
Published: July 2023

A study has been made of the penetration of 65Zn from various zinc chloride solutions, from a zinc oxide suspension and from a zinc tape containing zinc oxide through the intact skin of rats. 65Zn rapidly appeared in the blood and other tissues. The maximum 65Zn activity in serum occurred within or around the first hour after the application of both zinc chloride and zinc oxide almost completely independent of the zinc concentration applied and the pH. A greater total penetration of 65Zn was found from a carrier-free 65Zn-zinc chloride solution at pH 1 than from the same solution made less acidic (pH 4) and from a zinc oxide suspension at pH 8 (125 microgram Zn/ml). The 65 Zn penetration from the carrier-free solution was slightly (though not significantly) higher than that from the same solution with zinc chloride added to bring the total zinc concentration to 125 microgram/ml. Autoradiographically, 65Zn activity was seen in both dermis and panniculus carnosus. The activity was most concentrated on and near the epidermis and around hair follicles in dermis. No differences were observed between animals treated with zinc chloride (pH 1 and pH 4) and animals treated with a zinc oxide suspension.
Binding specificity of rabbit anti-guinea pig epidermal cell sera: comparison of their receptors with those of concanavalin A and pemphigus sera
Authors: Imamura S , Takigawa M , Ofuji S.
Published: July 2023

Rabbit anti-guinea pig epidermal cell sera (AES) which were prepared by immunizing rabbits with enzymatically dispersed viable guinea pig epidermal cells were shown to react to the intercellular substances of stratified squamous epithelia of guinea pigs and monkeys in a pattern similar to that seen with concanavalin A (ConA) or pemphigus sera (PS) by immunofluorescence. Reciprocal blocking tests were performed on guinea pig lip mucosa after removal of the non-specific binding substances between preincubated substances and subsequently incubated ones. No definite inhibition was noted in the subsequent reactions upon preincubation with AES or PS. However, preincubation with ConA weakened the subsequent reaction with PS, but did not block the reaction with AES. Effects of solvents on the receptors for AES, ConA and PS were also examined. ConA receptor was resistant to both saline solution and ethanol (95% and 99%). PS receptor was labile to both saline solution and ethanol, while AES receptor was labile to ethanol, but resistant to saline solution. These observations suggest that the receptor for AES differs from that for ConA or PS.
Measurement and differentiation of the cellular infiltrate in expermiental allergic contact dermatitis
Authors: Groth O , Skoog ML.
Published: July 2023

We describe a quantitative method for the grading of contact allergic reactions in guinea pigs. These reactions are characterized by marked cellular infiltration, and the method is based on total and differential counting of cells in the upper corium. A varying and objectively gradable increase in mononuclear and basophil polymorphonuclear cells was found. In naked-eye-positive reactions this increase was highly significant 24, 48, and 72 hours after epicutaneous application of dinitrochlorobenzene (DNCB). The degree of cellular infiltration reflects aspects of a cell-mediated immune response other than the visible reaction ordinarily made use of. The method can be used to study how systemically or topically administered drugs affect cellular features in contact allergy.
'Rusters'. The corrosive action of palmar sweat: II. Physical and chemical factors in palmar hyperhidrosis
Authors: Jensen O , Nielsen E.
Published: July 2023

When measuring sweating rates, close correspondence was found with the clinical estimation of hyperhidrosis. Corrosion was seen to increase with increasing sweat rates, reaching its maximum after an assumed rise in the actual sodium chloride concentration on the skin surface due to evaporation of water. The findings confirm that hyperhidrosis is of primary importance in the constitution of a 'ruster', and are also in good agreement with experimental reports. The small variations in palmar skin pH had no influence on the degree of corrosion; nor had the character of the metal surface. Of the two types of metal studied. corrosion was much more severe on the type having the lowest concentration of copper, thus confirming that increasing copper concentrations have a positive effect in reducing corrosion rates. At 50--60% relative humidity (RH) corrosion increased as time elapsed, whereas at 40% RH no corrosion developed on a sweat-contaminated plate. When exposed to 75% RH, metal samples became severely corroded in the course of a few days. Protective methods for the avoidance of rust are mentioned, with special emphasis on frequent handwashing.
Lack of dermal changes in uninvolved skin after photochemotherapy of psoriasis: biochemical studies
Authors: Blumenkrantz N , Weismann K , Asboe-Hansen G.
Published: July 2023

Low Hyp and Hyl values were found in psoriatic lesions. Treatment with PUVA achieved a return to normal values. No effect of PUVA on the collagen of uninvolved skin of psoriatic patients could be demonstrated after 4 months. There were no age-related differences in the content of Hyp and Hyl between samples of uninvolved skin of psoriatics.
Dermabrasion in Darier's disease
Authors: Zachariae H.
Published: July 2023

Dermabrasion was carried out in 5 patients with severe Darier's disease. All patients improved significantly within the treated areas. In general, over 75% of the dermabraded skin remained free of disease for more than 2-1/2 years after surgery. It is suggested that skinplaning should include the entire papillary dermis in order to obtain the beneficial effect.
Epidermolysis bullosa acquisita versus pemphigoid
Authors: Dahl MG.
Published: July 2023

Abstract is not available.
Dermatological manifestations in psoriatic arthritis: a follow-up study
Authors: Wright V , Roberts MC , Hill AG.
Published: July 2023

Of 227 patients with psoriasis and rheumatic complaints, inflammatory arthritis was present in 168 patients, of whom 95 have been followed up for more than 5 years and 28 had been followed up for more than 10 years. In patients with psoriasis and inflammatory arthritis the majority were women, but in the Distal joint group, males predominated. The skin disease usually began on the arms and was restricted in extent, initially. It usually began before the arthritis. A few cases were apparently precipitated by trauma, but more by psychological factors. The course pursued by the skin lesions was generally favourable. The skin lesions were resistant to treatment in 20% of the patients. Puberty, pregnancy and the menopause had little effect on the skin lesions. Nail lesions were present in 80%, a greater frequency than that seen in uncomplicated psoriasis. These nail lesions began at a later age than the skin lesions, and commonly preceded the development of arthritis. There was a family history of psoriasis in 26% of first-degree relatives. A history of polyarthritis was obtained quite frequently, and of psoriatic arthritis in 3% of patients with Distal arthritis, and 2% of the Indistinguishable group. The arthritis was usually mild in degree. Ankylosing spondylitis occurred in 5% and sacro-iliitis in 19%. The sheep cell agglutination test for rheumatoid factor was negative in the majority of patients. It was positive in 16% and a fluctuating positive result was obtained in 10%.
Fibrinogen-fibrin degradation products in exudates from bullous dematoses
Authors: Mortensen T , Clemmensen I , Søndergaard J.
Published: July 2023

Inflammatory exudates from 10 patients with bullous skin diseases were analysed by immunochemical techniques including crossed immunoelectrophoresis. The results were compared with those obtained in fluid from suction bullae obtained in normal skin in 13 control subjects and synovial fluid from 20 patients with rheumatoid arthritis. Abnormal fibrinogen degradation products identical with those found in synovial fluid from patients with rheumatoid arthritis were detected in exudates from each of the patients with bullous dermatoses, whereas significantly smaller amounts of fibrinogen antigenic material were detected in fluid obtained by suction. The fibrinogen antigenic material demonstrated in exudates from pathological bullae, immunochemically similar to that found in rheumatoid synovial fluid, indicates that the presence of these products reflects the more general features of an inflammatory exudate.
Acquired cutis laxa (generalized elastolysis): light and electron microscopic studies
Authors: Nanko H , Jepsen LV , Zachariae H , Søgaard H.
Published: July 2023

The case is described of a 44-year-old man with acquired cutis laxa. The primary clinical manifestation was an erythema of the chest. Gradually there developed persistent circumscribed lesions in other areas of the skin, spreading into large erythematous plaques with loose skin. Slight restrictive ventilatory insufficiency, elongation of the vocal cord and diverticulosis of the colon were also found, indicating a chronic and widespread disorder. Skin biopsies showed diminution and degeneration of the elastic fibres. The light-microscopic features were subdivided in relation to the various skin manifestations. Ultrastructure of the skin showed various amounts of electron-dense, amorphous material and loosely bound microfibrils in and around elastic and collagen fibres as well as some microfibrils with knobs in the interfibrouer, were found normal. It is suggested that the first changes in the elastic fibres induced an inflammatory response and mild mucinous changes of the collagen fibres. Furthermore elastic fibres seemed to be replaced by newly formed collagen.
Congenital poikiloderma with traumatic bulla formation, anhidrosisi, and keratoderma
Authors: Person JR , Perry HO.
Published: July 2023

A 14-year-old boy with congenital poikiloderma had anhidrosis, palmoplantar-pitted keratoderma, traumatic bulla formation, and defective dentition, but no abnormalities of the hair, nails, or eyes. This patient was similar in some respects to others reported as having dermatopathia pigmentosa reticularis, the Franceschetti-Jadassohn syndrome, the Mendes da Costa syndrome, and acrokeratotic poikiloderma.
Inflammatory linear verrucous epidermal nevus (ILVEN)
Authors: Skoven I.
Published: July 2023

A 12-year-old boy with inflammatory linear verrucous nevus is described. The lesions were pruritic and consisted of small, erythematous, slightly scaling papules coalescing to form linear, lichenified and excoriated plaques. The histological picture was psoriasiform but features of eczema were also seen.
Liver injury following administration of 8-methoxypsoralen during PUVA therapy
Authors: Bjellerup M , Bruze M , Hansson A , Krook G , Ljunggren B.
Published: July 2023

A case of liver injury caused by 8-methoxypsoralen given orally during PUVA therapy is presented. The reaction, manifested by elevated serum alanine-aminotransferase and serum aspartate-aminotransferase, was provoked on three consecutive occasions, on the last one with 8-MOP only. The liver injury seems to be of the hepatocellular and nonpredictable type.
Acquired epidermolysis bullosa treated with a gold compound
Authors: Livden JK , Nilsen R.
Published: July 2023

Abstract is not available.
On the significance of the trichophytin reactivity in atopic dermatitis
Authors: Rajka G , Barlinn C.
Published: July 2023

Patients with pure atopic dermatitis with and without tinea infection were investigated and compared with patients with long-lasting tinea infections and with controls, for presence of intracutaneous reactivity to trichoptin, Penicillium, Cladosporium and Alternaria antigens. RAST to Penicillium and Cladosporium was also performed. The results showed a lack of delayed reactivity, but an immediate reactivity to trichophytin in 50%/40% of atopic patients with/without tinea infection. Non-atopics infected with tinea showed 66% immediate and 33% delayed response to trichophytin. The reactivities in atopic dermatitis (but not in the non-atopic group) went general parallel with mould reactions tested intracutaneously or by RAST. It is assumed that a positive trichophytin reaction in atopic dermatitis does not necessarily mean sensitisation to dermatophytes, but is primarily the sign of a cross sensitivity to moulds.
Diffuse melanosis and trichochromuria in malignant melanoma
Authors: Agrup G , Agrup P , Hansson C , Rorsman H , Rosengren E.
Published: July 2023

A previous finding of pronounced excretion of trichochromes in a patient with metastasizing melanoma and diffuse melanosis prompted this study on trichochromes in another patient with these two features. The patient now reported also excreted large amounts of trichochromes. Iit is suggested that diffuse melanosis in metastatic melanoma patients is due to deposition of trichochromes in the tissue.
Diffuse cutaneous mastocytosis: a report of neonatal onset
Authors: Harrison PV , Cook LJ , Lake HJ , Shuster S.
Published: July 2023

Abstract is not available.
Tumour stage of mycosis fungoides treated with bleomycin and methotrexate: report from the Scandinavian mycosis fungoides study group
Authors: Groth O , Molin L , Thomsen K.
Published: July 2023

The Scandinavian Mycosis Fungoides Study Group have treated 19 patients with mycosis fungoides in tumour stage by systemic chemotherapy. Nine patients were treated with Bleomycin 15 mg i.m. twice weekly for 7 weeks and 10 patients with the same dose of Bleomycin in combination with Methotrexate 15 mg i.m. per m2 body surface each week for 7 weeks. No maintenance treatment was given. The immediate therapeutic effect of Bleomycin alone was considered good in half of the patients. Bleomycin and Methotrexate together produced a better initial effect. In both treatment series the remission was short-lived in the absence of maintenance therapy. The mortality rate was high, especially in combination treatment. Lethal complications occurred in 6 patients during the treatment, 3 of which were thromboembolic, one pancytopenia, one lung fibrosis with pulmonary insufficiency, and one bronchopneumonia. The conclusion is that these two forms of therapy cannot be recommended.
Keratinocyte and epidermal leukocyte expression of CD36 (OKM5) in benign and malignant skin diseases
Authors: Lisby S , Ralfkiaer E , Hansen E R , Vejlsgaard GL
Published: July 2023

CD36 recognizes a 88 Kd glycoprotein, found on different cell populations involved in immunoregulation and are induced on keratinocytes by in vitro treatment with gamma-interferon. Therefore, we obtained skin biopsies from 48 patients with various dermatological diseases and from 5 healthy volunteers and stained these with monoclonal antibodies OKM5 (CD36), anti-HLA-DR and OKT6 (CD1a) using a three stage immunoperoxidase method. In normal skin, CD36 expression was not observed. In contrast, keratinocytes in diseased skin were CD36+. In most cases, the staining was restricted to the stratum granulosum and the stratum spinosum, but in psoriasis, squamous cell carcinoma and lymphomatoid papulosis, more extensive staining of keratinocytes was seen. In addition, CD36+ epidermal leukocytes were found in allergic patch-test infiltrates and in mycosis fungoides. The findings of CD36 expression by epidermal cells within a broad spectrum of dermatological diseases indicate a role for these cells in the regulation of immune reactions in the skin.