bluuebunny/crossref_metadata_2025 huggingface dataset:
Patterns of nucleotide diversity for different domains of centromeric histone H3 (CENH3) gene in Secale L.
Authors: Gatzkaya S. S. , Evtushenko E. V.
Published: March 2019

Rye (Secale) is among staple cereals along with other members of the Triticeae tribe: wheat and barley. The genus Secale includes perennial and annual, cross-pollinating and self-pollinating species, and they can be donors of valuable genes in wheat and rye breeding programs. Studies of the structure of the gene for centromeric histone H3 (CENH3), essential for centromere functions, are relevant to the breeding of agronomically important crops. We have investigated the nucleotide diversity of sequences of two variants of the rye CENH3 gene inside the N-terminal tail (NTT) and the conservative HFD (histone fold domain) domain in the genus Secale. The mean values of nucleotide diversity in the NTT and HFD of wild cross- and self-pollinating taxa are close in αCENH3: πtot = 0.0176–0.0090 and 0.0136–0. 0052, respectively. In the case of βCENH3, the mean values for NTT (πtot = 0.0168–0.0062) are lower than for HFD (πtot = 0.0259–0.084). The estimates of nucleotide and haplotype diversity per site for the CENH3 domains are considerably lower in taxa with narrow geographic ranges: S. cereale subsp. dighoricum and S. strictum subsp. kuprijanovii. Commercial breeding reduces the nucleotide sequence variability in αCENH3 and βCENH3. Cultivated rye varieties have π values within 0.0122–0.0014. The nucleotide and haplotype diversity values in αCENH3 and βCENH3 are close in S. sylvestre, which is believed to be the oldest rye species. The results of this study prove that the frequency of single nucleotide polymorphisms and nucleotide diversity of sequences in genes for CENH3 in Secale species are influenced by numerous factors, including reproduction habits, the geographic isolation of taxa, breeding, and the evolutionary age of species.
ADF1 and BEAF-32 chromatin proteins affect nucleosome positioning and DNA decompaction in 61C7/C8 interband region of Drosophila melanogaster polytene chromosomes
Authors: Schwartz (Berkaeva) M. B. , Pankova T. E. , Demakov S. A.
Published: March 2019

The formation of interphase chromosomes is a multi-level process in which DNA is compacted several thousandfold by association with histones and non-histone proteins. The first step of compaction includes the formation of nucleosomes – the basic repeating units of chromatin. Further packaging occurs due to DNA binding to histone H1 and non-histone proteins involved in enhancer-promoter and insulator interactions. Under these conditions, the genome retains its functionality due to the dynamic and uneven DNA compaction along the chromatin fiber. Since the DNA compaction level affects the transcription activity of a certain genomic region, it is important to understand the interplay between the factors acting at different levels of the packaging process. Drosophila polytene chromosomes are an excellent model system for studying the molecular mechanisms that determine DNA compaction degree. The unevenness of DNA packaging along the chromatin fiber is easily observed along these chromosomes due to their large size and specific banding pattern. The purpose of this study was to figure out the role of two non-histone regulatory proteins, ADF1 and BEAF-32, in the DNA packaging process from nucleosome positioning to the establishment of the final chromosome structure. We studied the impact of mutations that affect ADF1 and BEAF-32 binding sites on the formation of 61C7/C8 interband – one of the decompacted regions of Drosophila polytene chromosomes. We show that such mutations led to the collapse of an interband, which was accompanied with increased nucleosome stability. We also find that ADF1 and BEAF-32 binding sites are essential for the rescue of lethality caused by the null allele of bantam microRNA gene located in the region 61C7/C8.
Generation of barcoded plasmid libraries for massively parallel analysis of chromatin position effects
Authors: Lebedev M. O. , Yarinich L. A. , Ivankin A. V. , Pindyurin A. V.
Published: March 2019

The discovery of the position effect variegation phenomenon and the subsequent comprehensive analysis of its molecular mechanisms led to understanding that the local chromatin composition has a dramatic effect on gene activity. To study this effect in a high-throughput mode and at the genome-wide level, the Thousands of Reporters Integrated in Parallel (TRIP) approach based on the usage of barcoded reporter gene constructs was recently developed. Here we describe the construction and quality checks of high-diversity barcoded plasmid libraries supposed to be used for high-throughput analysis of chromatin position effects in Drosophila cells. First, we highlight the critical parameters that should be considered in the generation of barcoded plasmid libraries and introduce a simple method to assess the diversity of random sequences (barcodes) of synthetic oligonucleotides using PCR amplification followed by Sanger sequencing. Second, we compare the conventional restriction-ligation method with the Gibson assembly approach for cloning barcodes into the same plasmid vector. Third, we provide optimized parameters for the construction of barcoded plasmid libraries, such as the vector : insert ratio in the Gibson assembly reaction and the voltage used for electroporation of bacterial cells with ligation products. We also compare different approaches to check the quality of barcoded plasmid libraries. Finally, we briefly describe alternative approaches that can be used for the generation of such libraries. Importantly, all improvements and modifications of the techniques described here can be applied to a wide range of experiments involving barcoded plasmid libraries.
Mechanisms of transcriptional regulation of ecdysone response
Authors: Mazina M. Yu. , Vorobyeva N. E.
Published: March 2019

The mechanisms of ecdysone-dependent expression have been studied for many decades. Initially, the activation of individual genes under the influence of ecdysone was studied on the model of polythene chromosomes from salivary glands of Drosophila melanogaster. These works helped to investigate the many aspects of the Drosophila development. They also revealed plenty of valuable information regarding the fundamental mechanisms controlling the genes’ work. Many years ago, a model describing the process of gene activation by ecdysone, named after the author – Ashburner model – was proposed. This model is still considered an excellent description of the ecdysone cascade, which is implemented in the salivary glands during the formation of the Drosophila pupa. However, these days there is an opinion that the response of cells to the hormone ecdysone can develop with significant differences, depending on the type of cells. The same genes can be activated or repressed under the influence of ecdysone in different tissues. Likely, certain DNA-binding transcription factors that are involved in the ecdysonedependent response together with the EcR/Usp heterodimer are responsible for cell-type specificity. A number of transcriptional regulators involved in the ecdysone response have been described. Among them are several complexes responsible for chromatin remodeling and modification. It has been shown by various methods that ecdysone-dependent activation/repression of gene transcription develops with significant structural changes of chromatin on regulatory elements. The description of the molecular mechanism of this process, in particular, the role of individual proteins in it, as well as structural interactions between various regulatory elements is a matter of the future. This review is aimed to discuss the available information regarding the main regulators that interact with the ecdysone receptor. We provide a brief description of the regulator’s participation in the ecdysone response and links to the corresponding study. We also discuss general aspects of the mechanism of ecdysone-dependent regulation and highlight the most promising points for further research.
The problems of genetic support of dividing the black kite (Milvus migrans) into subspecies
Authors: Andreyenkova N. G. , Starikov I. J. , Wink M. , Karyakin I. V. , Andreyenkov O. V. , Zhimulev I. F.
Published: March 2019

The black kite Milvus migrans is a common bird of prey demonstrating remarkable ecological plasticity. It inhabits a variety of habitats and is an increasingly synanthropic species. The black kite is widespread in Eurasia, Africa, Australia and adjacent islands. Palearctic kites migrate to Africa, India and China in winter, but kites of Africa and Australia are partly sedentary and partly seasonal migrants. The wide range and high mobility are the reasons of a complex population structure of the black kite. Commonly five to seven M. migrans subspecies are distinguished, each of which is widespread over extensive areas and has more or less an apparent phenotype. Recently, studies of genetic differences between black kite populations started to emerge. On the grounds of earlier studies of mitochondrial and nuclear genes of this species, we check whether there is a genetic support for separation of the black kite subspecies. Recent studies of some mitochondrial loci substantiate the recognition of at least the European (M. m. migrans), Asian (M. m. lineatus and M. m. govinda), African (M. m. aegyptius and M. m. parasitus), and Australian (M. m. affinis) black kite subspecies. Furthermore, the mitochondrial haplotype difference suggests that the African yellow-billed kite, including M. m. aegyptius and M. m. parasitus, should be a separate species as already proposed, or even two separate species.
Runs of homozygosity in spontaneous abortions from families with recurrent pregnancy loss
Authors: Skryabin N. A. , Vasilyev S. A. , Nikitina T. V. , Zhigalina D. I. , Savchenko R. R. , Babushkina N. P. , Lopatkina M. E. , Kashevarova A. A. , Lebedev I. N.
Published: March 2019

Recurrent pregnancy loss (RPL) is a severe reproductive pathology with a significant component of unexplained etiology. Extended homozygous regions as a possible etiological factor for RPL were sought in the genomes of embryos. Twenty-two paired first-trimester spontaneously aborted embryos from eleven women with recurrent miscarriage were analyzed. All embryos had normal karyotypes according to metaphase karyotyping and conventional comparative genomic hybridization. SurePrint G3 Human CGH + SNP 4 × 180K microarrays (Agilent Technologies) were used to search for homozygous regions. As a result, 39 runs of homozygosity (ROH) were identified in extraembryonic tissues of 15 abortuses. Verification of recurrent homozygous regions was performed by Sanger sequencing. The presence of occasional heterozygous SNPs was shown in 25 extended ROHs, which may indicate that they did not arise de novo but were inherited from parents. In the course of inheritance in a series of generations, they may accumulate mutations, leading to heterozygosity for several sites in the initially homozygous population-specific regions. Homozygotization of recessive mutations is one of the putative mechanisms of the influence of such inherited ROHs on RPL development. The high frequency of extended ROHs detected in the present study may point to a role of inbreeding in RPL etiology. Homozygous regions may also occur due to uniparental disomy, and abnormalities of genomic imprinting may be another mechanism responsible for the pathological manifestation of ROHs in embryogenesis. Indeed, five predicted imprinted genes were identified within ROHs according to the Geneimprint database: OBSCN, HIST3H2BB, LMX1B, CELF4, and FAM59A. This work reports the first finding of a high frequency of extended ROHs in spontaneously aborted embryos with normal karyotypes from families with RPL.
Matrix metalloproteinases in patients with resistant hypertension and type 2 diabetes mellitus: relation with renal blood ﬂow and kidney function
Authors: Falkovskaya A. Yu. , Mordovin V. F. , Pekarskiy S. E. , Ripp T. M. , Zyubanova I. V. , Sitkova E. S. , Lichikaki V. A. , Manukyan M. A. , Suslova T. E. , Gusakova A. M. , Ryabova T. R.
Published: March 2019

Objective.To evaluate the plasma levels of metalloproteinases (MMP) and their inhibitor in patients with resistant hypertension (RHTN) and type 2 diabetes mellitus (DM2) and their relationship with renal blood ﬂow and kidney function.

Design and methods.The study included 18 patients with RHTN and DM2 and 16 individuals with RHTN without DM, who underwent offce and 24-h ambulatory blood pressure measurements, renal Doppler ultrasound, and laboratory tests (daily albuminuria, estimated glomerular fltration rate calculated by CKD-EPI formula, HbА1c and basal glycaemia levels, MMP-9, MMP-2, tissue inhibitor of MMP type 1 (TIMP1), MMP/TIMP-1 ratios).

Results.Patients with RHTN and DM2 and subjects with RHTN without DM were comparable by mean levels of metalloproteinases, TIMP-1, and their ratios. The frequency of increased MMP-9 level was similar in both groups (61 % for RHTN + DM2 group and 75 % for RHTN without DM, p > 0,05). In patients with RHTN and DM2 the increase in MMP-9 and a decrease in TIMP-1/MMP-9 ratio were associated with a reduction in renal resistive indices (MMP-9 and RI in the main renal arteries: right-sided R = –0,60, p = 0,009, left-sided R = –0,60, p = 0,008; in segmental arteries: R = –0,49, p = 0,038 on the right and R = –0,59, p = 0,012 on the left; for TIMP-1/MMP-9 and segmental arteries: R = 0,51, p = 0,028 on the right; and R = 0,46, p = 0,04 on the left). The eGFR and MMP-9 signifcantly correlated (R = 0,55, p = 0,023). Patients with increased albuminuria showed higher values of TIMP-1/MMP-2 ratio than patients with normal albuminuria (2,97 ± 0,82 и 1,58 ± 0,33 ng/mL, respectively, p = 0,03). There was a direct correlation between TIMP-1/MMP-2 ratio and basal glycaemia (R = 0,59, p = 0,018).

Conclusions.Patients with RHTN and DM2 had comparable MMP levels and their inhibitor to those in patients with RHTN without DM, which may partly reﬂect that extracellular matrix formation is independent from carbohydrate disorders in RHTN. In patients with RHTN and DM2 an increase in MMP-9 and a decrease in TIMP-1/MMP-9 ratio may be adaptive, since it is accompanied by improved intrarenal blood ﬂow and renal fltration function. The increase in basal hyperglycemia is associated with the suppression of the proteolytic activity of MMP-2 related to the progression of albuminuria.
Kidney damage in essential arterial hypertension: pathogenetic issues for early diagnostics
Authors: Melnikova L. V. , Osipova E. V.
Published: March 2019

We review main issues of early diagnostics of kidney damage in patients with essential hypertension. The remodeling of renal vessels and the underlying mechanisms are discussed. The evidence-based data are reviewed to substantiate the use of laboratory methods for the kidney damage diagnostics (calculation of glomerular fltration rate and microalbuminuria). We discuss the role of Doppler methods in the assessment of intrarenal hemodynamics (the resistance index and blood ﬂow acceleration time) for timely detection of changes in renal vessels and the choice of management strategy.
Evaluation of blood pressure using Korotkoff’s sounds in case of auscultatory gap
Authors: Tikhonenko V. M. , Pivovarov V. V. , Kormilitsyn A. Y. , Zaitsev G. К.
Published: March 2019

Objective. To assess the clinical signifcance of auscultatory gap (AG) found by the 24-hour ambulatory blood pressure monitoring (ABPM) using the Korotkoff’s sounds (KS) and its potential causes.

Design and methods. 24-hour ABPM was performed in 60 patients (mean age 51,3 years, range 22–85 years), including 24 women and 36 men. In 24 patients, hypertension was diagnosed. In total, 2333 blood pressure (BP) measurements without “noise contamination” were selected for subsequent analysis (38,9 measurements per patient; from 28 to 73 measurements). The simultaneous recording of electrocardiogram (ECG), brachial cuff pressure, and KS phonograms on the right hand, as well as continuous recording of non-invasive BP curve in the left fnger using the volume clamp technique were performed in 10 patients with AG.

Results. Based on the 24-hour ABPM, AG was shown in 43 of 60 subjects (71,7%). The AG was found to be associated with a decreased amplitude of ﬂuctuations of the cuff pressure; upon its termination, the amplitude recovered. The above synchrony of changes in KS and amplitude of pressure ﬂuctuations was found in all 124 cases which demonstrated AG during BP measurement. We assume that AG is a true variation of BP rather than a feature of KS, as the ﬂuctuation amplitude depends on the ratio of BP and cuff pressure but does not depend on the KS phase. To test the hypothesis, the cuff pressure and KS were compared to the continuously monitored arterial pressure. When the systolic arterial pressure exceeded the cuff pressure, the KS appeared. Later on, the BP ﬂuctuations led to a decrease in systolic BP; the arterial pressure did not reach the cuff pressure and, therefore, KS were not recorded. Subsequent decrease in the cuff pressure led to re-appearance of KS.

Conclusions. The auscultative gap phenomenon is associated with true (mostly respiratory) variations of systolic BP rather than with features of KS in individual patients. The initial and repetitive KS occur at certain systolic BP levels depensing on the BP respiratory ﬂuctuations and may indirectly indicate the amplitude of ﬂuctuations.
30 YEARS ON THE BORDER OF INNOVATION IN OPHTHALMOLOGY
Authors: Fokin V. P , Boriskina L. N , Balalin S. V. , Yuferov O. V.
Published: March 2019

30 years activity of the Volgograd branch of FSAI NMRC ISTC «Eye Microsurgery named after academician S.N. Fedorov» of Public Health Ministry of the Russian Federation is constantly aimed at improving the efficiency and safety of treating eye diseases which is possible only if innovative technologies are developed and introduced into the practical work of the regional ophthalmological service. The period 1988-2018 of the ophthalmic care provision was analyzed. There were performed more than 610000 laser and surgical operations during this period. Fulfillment of work on the territorial state guarantees program for compulsory medical insurance, application of modern diagnostics methods, monitoring and treatment made it possible to increase the efficiency and availability of primary, specialized, high-tech ophthalmic care.
USE OF LATANOPROST INTHETREATMENT OF PROGRESSIVE OF MYOPIA
Authors: Trufanova L. P , Balalin S. V
Published: March 2019

There was performed a study of 36 patients with myopia after scleroplasty (36 eyes). The patients' age is from 5 to 17 years old, average age is (13,4 ± 2,5) years. The combination of accommodation weakness, constantly redundant tension of accommodation (CRTA) and intraocular pressure values in the range of 18 to 23 mm Hg indicates the presence of ophthalmic-hypertensive syndrome of overstretching accommodation and the progression of myopia. The use of 0,005 % solution of Latanoprost (Prolatan) in the complex treatment of patients with myopia leads to normalization of intraocular pressure, increase of corneal hysteresis and rigidity index of corneoscleral tunic, as well as increase in the coefficient of accommodative response and decrease in the coefficient of the ciliary body microfluctuations.
Verification of pushover analysis for a long-span steel truss structure
Authors: Yin Yue , Wang Shuai , Fang Zheli
Published: March 2019

Pushover analysis has become an effective tool for seismic design of high-rise buildings under severe earthquakes. However, the applicability of traditional pushover analysis is often questioned for long-span structures due to their complex dynamic characteristics. In this paper, pushover analysis was adopted to determine the seismic behavior of a long-span steel truss structure under severe earthquakes. Load distributions were determined based on the fundamental modes for vertical and horizontal earthquakes respectively. Pushover curves were obtained by nonlinear static analysis. Target displacements were determined with capacity spectrum method. The maximum displacements and plastic hinge distributions determined by traditional pushover analysis agreed well with those by nonlinear time history analysis for both horizontal and vertical earthquake actions. It was then concluded that the seismic behavior of this kind of long-span steel truss structures can be evaluated by traditional pushover analysis accurately enough for practical design purpose.
Integrated yaw and rollover stability control of an off-road vehicle with mechanical elastic wheel
Authors: Li Haiqing , Zhao Youqun , Lin Fen , Xu Han
Published: March 2019

An integrated control algorithm of the differential braking and the active suspension to improve yaw and rollover stability of vehicles with mechanical elastic wheel (ME-Wheel) is developed. By simplifying the structure of ME-Wheel, a fitting tire model named brush model is constructed. Then, a nonlinear 8-DOF vehicle model with ME-Wheel is built up for rollover prevention, which utilizes a predictive load transfer ratio (PLTR) as the rollover index and a Kalman filter is used to eliminate the measurement noise. In order to design an integrated control algorithm, fuzzy proportional-integral-derivative (PID) methodology is adopted by simultaneous control of the yaw and roll motions. The proposed algorithm, based on the idea that makes yaw stability controller and roll stability controller work independently first, then unifies by way of weight according to fuzz control, after that, brake force distributor selects single efficient braking wheel to achieve yaw moment and one of the front braking wheels with varying brake pressure to achieve the desired brake torque and the wheel slip regulator is designed with sliding mode control technique to prevent the wheels from locking; and the active suspension system alters the stiffness of the active suspension to prevent rollover. Simulation results show that the integrated yaw and rollover stability control system could improve the handing stability of vehicle under the limit driving conditions, and prevent rollover happening.
Study on an elastic lever system for electromagnetic energy harvesting from rail vibration
Authors: Wang Ping , Yang Fan , Gao Mingyuan , Wang Yuan
Published: March 2019

Energy harvesting from rail vibration is a promising approach to solve the power supply problem in remote and off-grid areas. The major issue of current energy harvesting techniques and devices is the limited power generating capacity. In this paper, the authors put forward an elastic lever system to enhance the performance of an electromagnetic energy harvester. A vehicle-track coupling dynamics model is established to simulate the service condition of the energy harvester. The Power Amplification Factor (PAF), which is defined as the ratio between the output power with and without a lever system, is introduced to quantify the Enhanced Energy Harvester (EEH). It is found that the PAF can be greater than n2 with a leverage ratio of n. Simulation shows that the output power can be magnified by 430 times with an elastic lever system with a leverage ratio of 10. The amplification effect of the output power comes from two aspects, one is the magnifying effect of leverage itself and the other is the resonance effect. Additionally, it is found that a single EEH will increase the wheel-rail contact force slightly, which indicates the EEH is impractical for use as an approach for rail vibration reduction. Nevertheless, it will not have a significant negative effect on rail vibration.
Design and implementation of solar power fed permanent magnet synchronous motor with improved DC-DC converter and power quality improvement using shunt active filter for reducing vibration in drive for industrial applications
Authors: Balasubramaniam Suresh , Gunasekaran Saravanakumar , Ramasamy Sampathkumar , Shanmugam Sathish Kumar
Published: March 2019

The research work proposes, Design and implementation of Solar power fed permanent magnet synchronous motor using improved DC-DC Converter and modified p-q theory based shunt active filter for reducing vibrations in drive for Industrial Applications. The Proposed research consists of both buck and boost converter, linking dc voltage unit and works in discontinuous conduction for boosting the battery life time. The improved converter provides multiple output capability using B4-inverter which reduces the cost of a proposed system considerably. In addition, for the reduction of harmonics in three phase system modified p-q module is enhanced. Comparing with the existing module, modified p-q module act as a triggering module for inverter to reduce harmonics in three phase systems and vibration across the motor, moreover the structured circuit would result in reduction of total harmonic distortion (THD), torque ripples, compact power switches, DC source reckoning and reduced starting current. The simulation of buck boost converter is considered, and its performance parameters were analyzed for different operating conditions. Thus, for industrial applications, the bidirectional converter and inverter fed permanent magnet synchronous motor drive is employed with the reduction in vibration, which is more efficient than conventional method. Here both simulation and experimental setup has been employed with a satisfying closed loop performance.
Experimental study of IERSFDs for vibration reduction of gear transmissions
Authors: Lu Kaihua , He Lidong , Yan Wei
Published: March 2019

Integral elastic ring squeeze film damper (IERSFD) is proposed to reduce and isolate the vibration of the gear transmissions. IERSFD offers the performance of a low radial stiffness and high squeeze film damping. This paper presents a mechanical model of an IERSFD elastic damping support and a single-degree-of-freedom vibration isolation system for the gear system. An open single-stage spur gear system was built to experimentally study the vibration characteristics of gear transmissions with rigid supports and with IERSFD elastic damping supports filled with damping fluids of different viscosities. The experimental results show that the IERSFD can effectively reduce the peak vibration acceleration of the gear shafts and can guarantee the smooth operation of the gear assembly over a range of speeds. This work shows that an IERSFD can reduce the shock and vibration of the gears’ meshing. It effectively attenuates vibration for most of the gears’ frequency components. Within a limited viscosity range, the vibration reduction is more effective with the higher-viscosity IERSFD damping fluids tested.
TEFL Methods in Indonesia
Authors: Anabokay Yanrini Martha , Suryasa I Wayan
Published: March 2019

The current research was a descriptive qualitative method that aimed to describe the TEFL methods applied by EFL teachers in Indonesia. Specifically, it aimed to know what the primary methods used by EFL teachers, how they delivered the methods, and what are the advantages and the disadvantages of applying the method in Indonesia case. The focus of the current research was on elementary teachers. Data were gained through interview and classroom observation. The research has shown that the primary methods used by EFL teachers were Grammar Translation Method and Direct Method. They combined those two methods and delivered them using English and Bahasa.
Ubuntu, gender and spirituality:
Authors: Nagel Mechthild
Published: March 2019

O princípio do Ubuntu, popularizado pelo arcebispo Desmond Tutu, presidindo a Comissão de Verdade e Reconciliação na Nova África do Sul, tem potencial para auxiliar as concepções filosóficas ocidentais do perdão, na visão da justiça transformadora. Aspectos do Ubuntu se justapõem à ética do cuidado, inspirada pelas feministas ocidentais, ao mesmo tempo em que se afastam da ética ocidental, com sua ênfase na espiritualidade e no comunalismo.
Vulnerabilidade e desigualdade moral de gênero:
Authors: Aparecida Kuhnen Tania
Published: March 2019

Ao partir da crítica de que as teorias morais tradicionais não se ocuparam dos problemas da desigualdade de gênero nos papeis morais, este artigo busca apresentar o cuidado como uma habilidade fundamental humana a ser desenvolvida pelo agente moral. Situa-se a condição de vulnerabilidade na base da abordagem moral, deslocando-se o agente moral autônomo do centro das teorias, para considerar o aspecto relacional do ser humano que, ao longo de sua vida, depara-se com diferentes dimensões da vulnerabildiade e é afetado por experiências de vulnerabilidade, de maior ou menor intensidade e duração. Defende-se a necessidade de formação de agentes morais completos, não mais separados por habilidades morais limitadas à atribuição de papeis sociais fixos orientados por gênero. A partir disso, explora-se uma complementaridade entre cuidado e direitos na formação do agente moral.
filosofia feminista de Luce Irigaray
Authors: Nancy Peña Cortés Olga
Published: March 2019

No âmbito da filosofia feminista francesa as discussões a respeito da mulher, do feminino e da feminilidade passam a compor o cenário intelectual nos anos sessenta, momento no qual a filosofia passava igualmente por processos de ruptura e novas problemáticas. Advindas das discussões promovidas, especialmente pelo estruturalismo, o campo filosófico vivencia nova ruptura com os movimentos feministas e o surgimento de novas concepções filosóficas. Nesse contexto salienta-se o feminismo da diferença apresentado pela filósofa, psicanalista, escritora e feminista belga Luce Irigaray. O presente artigo busca inicialmente traçar o projeto de sua filosofia feminista, o qual se encontra alicerçado na ausência da diferença sexual, pois afirma a inexistência do sujeito feminino enquanto feminino, salientando a existência de uma única lógica, a falocêntrica. O discurso do conhecimento é, entendido com um discurso sexuado, tema da conferência Le Sujet de la Science est-il sexué (1985), a partir da qual surge a pergunta a respeito do discurso filosófico, objeto das considerações finais.
Analisis Perancangan Sistem Informasi Administrasi Program Studi Pendidikan Teknologi Informasi dan Komunikasi
Authors: Sulistiyarini Dewi , Sabirin Febrianto
Published: March 2019

This study aims to determine the description of needs, data flow design, database design, and design of information systems administration interfaces PSTIK study program. The research used descriptive method with research form that is survey research. Research subjects are staff, leadership of study program and 60 students of study program. Data analysis technique is to describe the data collected. The results show that the administrative information system consists of seminars and theses, lecturer journals, incoming and outgoing letters, and reports that are tailored to functional requirements, interface needs, and performance needs. The flow of information system data consists of two entities ie students and staff. Students have 6 inflows and outflows, while staff has 10 inflows and outflows. The information system database consists of 16 entities with 8 interrelated relations. The information system interface is divided into two pages. The student page consists of 6 pages, while the staff page consists of three pages.
Malli käyttäjäkeskeisen suunnittelun johtamiseen julkisella sektorilla
Authors: Kautonen Heli
Published: March 2019

TaM Heli Kautonen väitteli 22.2.2019 Aalto-yliopiston perustieteiden korkeakoulussa aiheesta "BAIST-viitemalli julkista arvoa tuottavaan digitaaliseen palvelun käyttäjäkeskeiseen suunnitteluun". Vastaväittäjänä toimi professori Netta Iivari (Oulun yliopisto), ja kustoksena professori Marko Nieminen (Aalto-yliopiston perustieteiden korkeakoulu). Tietotekniikan alaan kuuluva väitöskirja on julkaistu sarjassa Aalto university publication series: doctoral dissertations, ja se on luettavissa myös Aalto-yliopiston Aaltodoc-julkaisuarkistossa osoitteessa http://urn.fi/URN:ISBN:978-952-60-8374-2.

Lektiota on kevyesti toimitettu kirjallisessa muodossa julkaisemista varten.
Acknowledgements
Authors: Puhakainen Tuula
Published: March 2019

Agricultural and Food Science expresses its sincere thanks to the referees for their constructive critical reviews of one or more manuscripts during the year 2018.
The Key Parameter Effect Analysis Of Polymer Flooding On Oil Recovery Using Reservoir Simulation
Authors: Erfando Tomi , Rita Novia , Ramadhan Romal
Published: March 2019

As time goes by, there will be decreasing of production rates of a field along with decreasing pressure. This led to the necessity for further efforts to increase oil production. Therefore, pressure support is required to improve the recovery factor. Supportable pressure that can be used can be either water flooding and polymer flooding. This study aims to compare recovery factor to scenarios carried out, such as polymer flooding with different concentrations modeled in the same reservoir model to see the most favorable scenario. The method used in this research is reservoir simulation method with Computer Modeling Group (CMG) STARS simulator. The study was carried out by observing at the pressure, injection rate, and polymer concentration on increasing field recovery factor. This study used cartesian grid with the assumption of homogeneous reservoir, there are no faults or other geological condition in the reservoir, and driving mechanism is only solution gas drive. This reservoir, oil type is light oil with API gravity 40.3˚API and layer of conglomerate rock. The simulation result performed with various scenarios provides a good result. Where the conditions case base case field recovery factor of 6.7%, and after water flooding produced 25.5% of oil, whereas with tertiary recovery method is polymer flooding was carried out with four concentrations of 640 ppm, 1,500 ppm, 3,000 ppm, and 4,000 ppm obtained optimum values at 4,000 ppm polymer concentration with recovery factor 28.9%, SOR reduction final value 0,5255, polymer adsorption of 818,700 ppm, reservoir final pressure 1,707 psi, and an increase in water viscosity to 0.94 cP.
An Analysis of the Accuracy of Time Domain 3D Image Geology Model Resulted from PSTM and Depth Domain 3D Image Geology Model Resulted from PSDM in Oil and Gas Exploration
Authors: Irawan Sudra , Rokhayati Yeni , Aji Satriya Bayu
Published: March 2019

This study aims to obtain a geological model which is close to the truth and compare accuracy between the time domain 3D image of the PSTM results with the depth domain 3D image of PSDM results. There are 3 parameters to determine the accuracy of an interval velocity model in the production of a geology model: depth gathering that is already flat, semblance that has concurred with zero residual move-out axes, and depth image which conforms to the marker (well seismic tie). The analytical method employed is Horizon Based Tomography, which is a method to correct the seismic wave travel time error along the analyzed horizon. Reducing errors in the travel time of the seismic wave will decrease depth errors. This improvement is expected to provide correct information about subsurface geological conditions. The results showed that the depth domain image generated by the PSDM process represents the actual geological model better than time domain image produced by the PSTM process, evidenced by the sharpening of the reflector continuity, reduction of pull-up effect, and high resolution.
Age and Paleobathymetry of Salodik Group in PohPagimana section, East Arm of Sulawesi Based on Foraminiferal Assemblages
Authors: Fakhruddin Rakhmat , Kurniadi Dedy
Published: March 2019

Planktonic and benthic foraminiferal assemblages were used for age and paleobathymetry analysis of outcropping carbonate succession of the Salodik Group in Poh–Pagimana section, East Arm of Sulawesi. Twenty spot samples of carbonate rocks were collected from cut slope along the road between Poh and Pagimana. Age analysis conducted for each sample by its planktonic foraminifera assemblages and group into their age interval. The age range from Early Eocene to Pliocene. Benthic foraminiferal assemblages indicate paleobathymetry ranging from middle shelf to upper bathyal settings. Age analysis of the studied section show an older sediments (Early–Middle Eocene) overlie younger sediment (Early Miocene). This is interpreted as the evidence of thrust fault in the Poh–Pagimana section as part of thrust sheets of Batui thrust complex which imbricated on deformation zone. The youngest sediment which have suffered deformations shown by disordered age sequence is Pliocene in age (N20-21). The Eocene to Pliocene carbonate succession in the studied section was deposited relatively in the more basinward position compared to the carbonate-dominated sediments in the Tomori area which was deposited in the more landward position.
Prediction of features of the course of chronic hepatitis C using Bayesian networks
Authors: Samokhodskaya L M , Starostina E E , Sulimov A V , Krasnova T N , Rosina T P , Avdeev V G , Savkin I A , Sulimov V B , Mukhin N A , Tkachuk V A , Sadovnichii V A
Published: March 2019

Materials and methods. 253 patients with chronic hepatitis C (CHC) and liver cirrhosis were included in the study. Assessment of gene polymorphisms of genes involved in inflammatory reactions and antiviral immunity (IL-1β-511C/T, IL-10 -1082G/A, IL28B C/T, IL28B T/G, TNF-α -238G/A, TGF-β -915G/C, IL-6 -174G/C), activators of local hepatic fibrosis (AGT G-6A, AGT 235 M/T, ATR1 1166 A/C), hemochromatosis (HFE C282Y, HFE H63D), platelet receptors (ITGA2 807 C/T, ITGB3 1565 T/C), coagulation proteins and endothelial dysfunction (FII 20210 G/A, FV 1691G/A, FVII 10976 G/A, FXIII 103 G/T, eNOS 894 G/T, CYBA 242 C/T, FBG -455 G/A, PAI-675 5G/4G, MTHFR 677 C/T) was carried. Using Bayesian networks we studied the predictor value of clinical and laboratory factors for the following conditions - end points (EP): development of cirrhosis (EP1), fibrosis rate (EP2), presence of portal hypertension (EP3) and cryoglobulins (EP4). Results and discussion. In addition to traditional factors we have shown the contribution of the following mutations. Predicting EP1- liver cirrhosis - HFE H63D, C282Y, CYBA 242 C/T, AGT G-6G, ITGB31565 T/C gene mutations were significant. We also found a link between the rate of progression of liver fibrosis and gene polymorphisms of AGT G-6G, AGT M235T, FV 1691G/A, ITGB31565 T/C. Among the genetic factors associated with portal hypertension there are gene polymorphisms of PAI-I-675 5G/4G, FII 20210 G/A, CYBA 242 C/T, HFE H63D and Il-6 174GC. Cryoglobulins and cryoglobuliemic vasculitis (EP4) are associated with gene mutations MTHFR C677T, ATR A1166C and HFE H63D. Conclusion. The results obtained allow to detect the major pathophysiological and genetic factors which determine the status of the patient and the outcome of the disease, to clarify their contribution, and to reveal the significance of point mutations of genes that control the main routes of HCV course and progression.
Risk factors of unfavorable prognosis of chronic hepatitis C
Authors: Shchanitcyna S E , Burnevich E Z , Nikulkina E N , Filatova A L , Moiseev S V , Mukhin N A
Published: March 2019

Aim. To investigate risk factors of unfavorable prognosis in patients with chronic hepatitis C (CHC), including liver cirrhosis (LC), decompensated cirrhosis, hepatocellular carcinoma (HCC), cryoglobulinemic vasculitis (CryoVas) and B-cell non-Hodgkin’s lymphoma. Materials and methods. This was a retrospective study using data of 824 patients with CHC hospitalized between 2010 and 2016 in clinic named after E.M. Tareev. We used multivariate analysis including logistic regression to calculate odds ratios (ORs) for potential risk factors/predictors associated with unfavorable outcomes in patients with CHC. Results and discussion. The rate of LC, decompensated LC, HCC, serious CryoVas and B-cell lymphoma in patients with CHC was 39.1% (322/824), 14.0% (115/824), 2.8% (23/824), 5.2% (43/824) and 1.2% (10/824), respectively. After adjustment for sex and age the rate of LC, decompensated LC, HCC was 22.8, 8.0 and 1.5%, respectively. Annual rate of LC in patients with CHC was 1.5%; in cirrhotic patients annual rate of decompensated LC and HCC was 2.9 and 1%, respectively. Risk factors independently associated with development of LC were elevated body mass index (OR 1.43), immunosuppressive therapy (OR 1.67), diabetes type 2 (OR 2.03), absence of antiviral therapy (OR 2.15), alcohol abuse (OR 2.34), duration of infection ≥20 years (ОR 2.74) and an absence of sustained virological responce (SVR) (OR 2.98). Independent risk factors for decompensation in cirrhotic patients included diabetes type 2 (OR 1.47), alcohol abuse (OR 1.53), an absence of antiviral therapy (OR 2.36) and an absence of SVR (OR 1.94). An episode of decompensation was the independent predictor of HCC in cirrhotic patients (OR 3.99). Genotype 1b (OR 1.66) and an absence of antiviral therapy (OR 3.31) were independently associated with serious CryoVas. Two prognostic scales were offered for risk evaluation of LC and its complications. Conclusions. Multivariate analysis showed several factors independently associated with higher risk for LC, decompensation of LC, HCC, serious CryoVas in patients with CHC. The rate of unfavorable outcomes of CHC is found, including rare extrahepatic manifestations.
Phenotype of obesity and gastroesophageal reflux disease in the context of comorbidity in patients with cardiovascular diseases
Authors: Maev I V , Yurenev G L , Mironova E M , Yureneva-Thorzhevskaya T V
Published: March 2019

The relevance of studying such problems as gastroesophageal reflux disease (GERD) and obesity is caused by their high prevalence in the developed countries of the world. Epidemiological data indicate that obesity is a significant risk factor for developing GERD due to increased intra-abdominal pressure and gastroesophageal gradient, slowing of gastric evacuation and formation of hiatal hernia. Abdominal obesity increases the likelihood of complications of GERD: erosive esophagitis, Barrett's esophagus and adenocarcinoma. This fact is connected with humoral influences: increased production of pro-inflammatory cytokines and leptin, and decreased secretion of adiponectin. Treatment of comorbid patients requires higher dosages and longer courses of antisecretory medicines, and an additional prescription of ursodeoxycholic acid.
Non-alcoholic fatty liver disease as a cause and consequence of cardio-metabolic complications. Role of the ursodeoxicholic acid in the pharmacotherapy
Authors: Maevskaya M V , Ivashkin V T , Ivashkin K V , Lunkov V D , Liusina E O , Zozula V N , Leshchenko V I
Published: March 2019

The article presents an update of the role of non-alcoholic fatty liver disease (NAFLD) in cardiometabolic diseases and events: arterial hypertension and components of the metabolic syndrome. A review of NAFLD modern pharmacotherapy has been conducted. Particular attention is paid to the place of ursodeoxycholic acid in the complex treatment of NAFLD.
Rebamipide increases the disaccharidases activity in patients with enteropathy with impaired membrane digestion. Pilot study
Authors: Parfenov A I , Belostotsky N I , Khomeriki S G , Akhmadullina O V , Bykova S V , Sabelnikova E A , Dbar S R
Published: March 2019

Aim. To evaluate the effectiveness of enteroprotector Rebamipide in the treatment of enteropathy with impaired membrane digestion (EIMD). Materials and methods. We examined 102 patients aged 18 to 50 years (41 men and 61 women) with clinical signs of irritable bowel syndrome (n=65), functional diarrhea (n=33), and functional constipation (n=4) according to Rome IV criteria (2016). The activities of glucoamylase (GA), maltase, sucrase and lactase were determined by Dahlquist-Trinder method in duodenal biopsies obtained during esophagogastroduodenoscopy. The control group consisted of 20 healthy people aged 23-47. They showed following average enzyme activity: lactase - 42±13 ng glucose on 1 mg of tissue per minute, GA - 509±176, maltase - 1735±446, sucrase - 136±35 ng glucose on 1 mg of tissue per minute. These numbers were taken as the norm. Results and discussion. The activity of the disaccharidases was reduced in 89.2% out of 102 patients, and they were diagnosed with EIMD. Thirteen patients with EIMD were recommended to maintain the FODMAP diet and take enteroprotector Rebamipide 100 mg 3 times a day for 12 weeks. After 3 months 11 patients reported decreased or no flatulence, abdominal pain, stool disorder; 2 patients reported no change. The activity of GA increased to an average of 149±82 (by 78%, p=0.016), maltase - to 864±472 (by 131%, p=0.0019), sucrase - 63±35 (by 95%, p=0.0041) and lactase - 10±8 ng glucose on 1 mg of tissue per minute. The activity of lactase did not change. Conclusion. We discovered a previously unknown phenomenon of the disaccharidases activity increase in duodenal mucosa and improved carbohydrates tolerance in the patients with EIMD taking Rebamipide in the dose 300 mg/day for 12 weeks.
Clinical efficacy of acute respiratory viral infections prevention in patients with chronic heart failure
Authors: Budnevsky A V , Shurupova A D , Kravchenko A Ya , Tokmachev R E
Published: March 2019

The aim of the study was to evaluate the ARVI prevention effectiveness in patients with chronic heart failure (CHF) using interferon inducer amixin. Materials and methods. Conducted a comprehensive survey, dynamic monitoring and treatment of 60 patients aged from 49 to 70 years (mean age 60.25±4.57 years, 17 men and 43 women) with CHF with preserved ejection fraction of left ventricle (LVEF) (≥50%), II-III functional class (FC) according to the classification of new York Heart Association (NYHA), which developed as a result of coronary heart disease (CHD), hypertensive disease (HD). Of these, 30 patients (group 1) on the background of standard therapy for CHF received for the prevention of ARVI tiloron (Amixin) at a dose of 125 mg once a week for 6 weeks, two courses for 1 year. Group 2 patients received only standard therapy for CHF. Results. A decrease in the frequency of ARVI in patients with CHF treated with Amixin was found, which was accompanied by a decrease in the severity of subclinical inflammation by reducing the production of proinflammatory (IL-1β) and increasing the production of anti-inflammatory (IL-10) cytokines, reducing neurohumoral activation (reducing levels of aldosterone and Nt-proBNP), increasing the level of α- and γ-interferon. The positive dynamics of biomarkers of systemic inflammation and neurohormonal activation explains the improvement of the clinical course in patients with CHF (increase of tolerance to physical loads, reducing the number of visits to General practitioner and hospital admissions in the hospital during 12 months of observation). Conclusion. A promising approach to the prevention of SARS in patients with CHF is course therapy with Amixin (2 times a year before the seasonal rising in the incidence of respiratory viral infections and influenza), which allows to achieve both decreasing in the frequency of SARS per year, and improvement the clinical course of CHF.
Program for TPDP 2016
Authors: Barthe Gilles , Dimitrakakis Christos , Gaboardi Marco , Haeberlen Andreas , Roth Aaron , Slavković Aleksandra B
Published: March 2019

The Theory and Practice of Differential Privacy workshop (TPDP 2016) was held in New York City, NY, USA on 23 June 2016 as part of ICML 2016. This is the program.
Vascular complication after anterior ankle arthroscopy
Authors: Santos Tatiana Ferreira dos , Matos e Dinato Mauro Cesar
Published: March 2019

Postoperative complications are a concern for surgeons, and their incidence reaches 9% in ankle arthroscopy. Of these, neurovascular complications are particularly concerning. This report aims to show a vascular complication not previously reported in the literature, in order to alert about its possibility and to provide information on the treatment recommendations in the current literature. It is a case of distal arterial occlusion in the foot after anterior ankle arthroscopy. The patient was operated upon, and the problem was resolved four months after surgery. Level of Evidence V; Therapeutic Studies; Expert Opinion.