bluuebunny/crossref_metadata_2025 huggingface dataset:
Identification of a Homozygous PEX26 Mutation in a Heimler Syndrome Patient
Authors: Kim Youn Jung , Abe Yuichi , Kim Young-Jae , Fujiki Yukio , Kim Jung-Wook
Published: April 2021

This study aimed to identify the molecular genetic etiology of an 8-year-old boy with amelogenesis imperfecta in permanent dentition. Bilateral cochlear implants were placed due to sensorineural hearing loss, and there was no other family member with a similar phenotype. Peripheral blood samples were collected with the understanding and written consent of the participating family members. A constitutional chromosome study was performed for the proband. Genomic DNA was isolated, and whole exome sequencing was performed. A series of bioinformatic analyses were performed with the obtained paired-end sequencing reads, and the variants were filtered and annotated with dbSNP147. There was no abnormality in the constitutional chromosome study. Whole exome sequencing analysis with trio samples identified a homozygous mutation (c.506T>C, p. (Leu169Pro)) in the PEX26 gene. We verified “temperature sensitivity (ts)” of patient-derived Pex26-L169P by expression in pex26 CHO mutant ZP167 cells to determine the effect of the L169P mutation on Pex26 function. The L169P mutation causes a mild ts-cellular phenotype representing the decreased peroxisomal import of catalase. This study supports the finding that the recessive mutations in PEX26 are associated with Heimler syndrome and demonstrates the importance of an early and correct diagnosis.
StalGrowth—A Program to Estimate Speleothem Growth Rates and Seasonal Growth Variations
Authors: Vieten Rolf , Hernandez Francisco
Published: April 2021

Speleothems are one of the few archives which allow us to reconstruct the terrestrial paleoclimate and help us to understand the important climate dynamics in inhabited regions of our planet. Their time of growth can be precisely dated by radiometric techniques, but unfortunately seasonal radiometric dating resolution is so far not feasible. Numerous cave environmental monitoring studies show evidence for significant seasonal variations in parameters influencing carbonate deposition (calcium-ion concentration, cave air pCO2, drip rate and temperature). Variations in speleothem deposition rates need to be known in order to correctly decipher the climate signal stored in the speleothem archive. StalGrowth is the first software to quantify growth rates based on cave monitoring results, detect growth seasonality and estimate the seasonal growth bias. It quickly plots the predicted speleothem growth rate together with the influencing cave environmental parameters to identify which parameter(s) cause changes in speleothem growth rate, and it can also identify periods of no growth. This new program has been applied to multiannual cave monitoring studies in Austria, Gibraltar, Puerto Rico and Texas, and it has identified two cases of seasonal varying speleothem growth.
Assessing Public Willingness to Wear Face Masks during the COVID-19 Pandemic: Fresh Insights from the Theory of Planned Behavior
Authors: Irfan Muhammad , Akhtar Nadeem , Ahmad Munir , Shahzad Farrukh , Elavarasan Rajvikram Madurai , Wu Haitao , Yang Chuxiao
Published: April 2021

Face masks are considered an effective intervention in controlling the spread of airborne viruses, as evidenced by the 2009′s H1N1 swine flu and 2003′s severe acute respiratory syndrome (SARS) outbreaks. However, research aiming to examine public willingness to wear (WTW) face masks in Pakistan are scarce. The current research aims to overcome this research void and contributes by expanding the theoretical mechanism of theory of planned behavior (TPB) to include three novel dimensions (risk perceptions of the pandemic, perceived benefits of face masks, and unavailability of face masks) to comprehensively analyze the factors that motivate people to, or inhibit people from, wearing face masks. The study is based on an inclusive questionnaire survey of a sample of 738 respondents in the provincial capitals of Pakistan, namely, Lahore, Peshawar, Karachi, Gilgit, and Quetta. Structural equation modeling (SEM) is used to analyze the proposed hypotheses. The results show that attitude, social norms, risk perceptions of the pandemic, and perceived benefits of face masks are the major influencing factors that positively affect public WTW face masks, whereas the cost of face masks and unavailability of face masks tend to have opposite effects. The results emphasize the need to enhance risk perceptions by publicizing the deadly effects of COVID-19 on the environment and society, ensure the availability of face masks at an affordable price, and make integrated and coherent efforts to highlight the benefits that face masks offer.
The Father in Youth Baseball: A Self-Determination Theory Approach
Authors: De La Cruz Manuel , Zamarripa Jorge , Castillo Isabel
Published: April 2021

This study based on the self-determination theory aims to examine the relationship among the aspirations that fathers have about their children’s youth baseball practice, the children’s basic psychological needs (satisfaction and frustration), and their intentions to either continue or drop out of baseball practice in a sample of children from Hermosillo, Mexico. A cross-sectional study was carried out involving 533 fathers (M = 44.30, SD = 5.18) and 533 children (M = 13.09, SD = 1.68). The results showed that the intrinsic aspirations of fathers were positively correlated to the satisfaction of the children’s psychological needs, whereas the extrinsic aspirations of fathers were positively correlated with the frustration of the children’s psychological needs. Satisfaction of basic psychological needs was positively correlated with the intention to continue and negatively correlated with dropout; on the contrary, frustration of basic psychological needs was negatively correlated with the intention to continue and positively with dropout. In conclusion, the fathers’ pursuit of intrinsic aspirations for their children in the youth baseball context satisfies the children’s basic psychological needs, and in turn, their intention to continue practicing increases. Conversely, when a father pursues extrinsic aspirations for his son in youth baseball, the child will feel his basic psychological needs frustrated, and he will have a greater intention to drop out. Overall, this study extends the existing sport-scientific literature by confirming the impact of parents’ aspirations on their children’s basic psychological needs and intention to continue being baseball players.
Exploring the Determinants of Repeated-Sprint Ability in Adult Women Soccer Players
Authors: Gonçalves Lillian , Clemente Filipe Manuel , Barrera Joel Ignacio , Sarmento Hugo , González-Fernández Francisco Tomás , Rico-González Markel , Carral José María Cancela
Published: April 2021

This study aimed to explore the main determinants of repeated-sprint ability (RSA) in women soccer players considering aerobic capacity, sprinting performance, change-of-direction, vertical height jump, and hip adductor/abductor isometric strength. Twenty-two women soccer players from the same team participating in the first Portuguese league were observed. Fitness assessments were performed three times during a 22-week cohort period. The following assessments were made: (i) hip abductor and adductor strength, (ii) squat and countermovement jump (height), (iii) change-of-direction test, (iv) linear sprinting at 10- and 30-m, (v) RSA test, and (vi) Yo-Yo intermittent recovery test level 1. Positive moderate correlations were found between peak minimum RSA and adductor and abductor strength (r = 0.51, p < 0.02 and r = 0.54, p < 0.01, respectively). Positive moderate correlations were also found between peak maximum RSA and adductor and abductor strength (r = 0.55, p < 0.02 and r = 0.46, p < 0.01, respectively). Lastly, a moderate negative correlation was found between fatigue index in RSA and YYIR1 test performance (r = −0.62, p < 0.004). In conclusion, abductor and adductor isometric strength-based coadjutant training programs, together with a high degree of aerobic endurance, may be suitable for inducing RSA in female soccer players.
Risk Factors for Unfavorable Treatment Outcomes among the Human Immunodeficiency Virus-Associated Tuberculosis Population in Tashkent City, Uzbekistan: 2013–2017
Authors: Massavirov Sherali , Akopyan Kristina , Abdugapparov Fazlkhan , Ciobanu Ana , Hovhanessyan Arax , Khodjaeva Mavluda , Gadoev Jamshid , Parpieva Nargiza
Published: April 2021

Tuberculosis (TB) and human immunodeficiency virus (HIV) co-infection poses a growing clinical challenge. People living with HIV have a higher chance of developing TB, and once the disease has progressed, are at greater risk of having unfavorable TB treatment outcomes. Data on TB treatment outcomes among the HIV-associated TB population in Uzbekistan are limited. Thus, we conducted a cohort study among 808 adult patients with HIV-associated TB registered at the Tashkent TB referral hospital from 2013–2017 to document baseline characteristics and evaluate risk factors for unfavorable TB treatment outcomes. The data were collected from medical records and ambulatory cards. About 79.8% of the study population had favorable treatment outcomes. Antiretroviral therapy (ART) coverage at the admission was 26.9%. Information on CD4-cell counts and viral loads were largely missing. Having extrapulmonary TB (aOR 2.21, 95% CI: 1.38–3.53, p = 0.001), positive sputum smear laboratory results on admission (aOR 1.62, 95% CI: 1.07–2.40), diabetes (aOR 5.16, 95% CI: 1.77–14.98), and hepatitis C (aOR 1.68, 95% CI: 1.14–2.46) were independent risk factors for developing unfavorable TB treatment outcomes. The study findings provide evidence for targeted clinical management in co-infected patients with risk factors. Strengthening the integration of TB/HIV services may improve availability of key data to improve co-infection management.
Patterns and Predictors of Sitting among Women from Disad-Vantaged Neighbourhoods over Time: A 5-Year Prospective Cohort Study
Authors: Nayak Minakshi , Wills Karen , Teychenne Megan , Salmon Jo , Cleland Verity
Published: April 2021

Background: Our aim was to describe patterns of sitting over time and determine the sociodemographic predictors of sitting over time among women living in socioeconomically disadvantaged neighbourhoods. Methods: Women age between 18 and 45 years (mean = 34.4 ±8.1, n = 4349) reported their sitting time, sociodemographic (e.g., age), and health (e.g., body mass index) three times over 5 years. Linear mixed modelling was used to determine the predictors of change in sitting over time, adjusting for covariates. Results: Mean baseline sitting time was 40.9 h/week, decreasing to 40.1 h/week over five years. Greater sitting time was reported in participants ≤25 years of age, living with obesity, living in urban areas, self-reported poor/fair health, working full-time, with higher education, never married and with no children. Annually, the average sitting time decreased by 0.4 h/week (95% CI; −0.7 to −0.05) in women working full-time but increased by 0.1 h/week (95% CI; −0.2 to 0.6) who were not working. Similarly, annual sitting time decreased by 0.6 h/week (95% CI; −0.2 to 1.3) in women with no children but increased by 0.4 h/week (95% CI; −0.2 to 0.5) and 0.9 h/week (95% CI; 0.3 to 1.3) among those with two and three/more children, respectively. Conclusion: Among disadvantaged women, those not working and with two or more children may be at particular risk for increased sitting time and warrant further attention.
Assessment of Back-Squat Performance at Submaximal Loads: Is the Reliability Affected by the Variable, Exercise Technique, or Repetition Criterion?
Authors: Pérez-Castilla Alejandro , Janicijevic Danica , Akyildiz Zeki , Senturk Deniz , García-Ramos Amador
Published: April 2021

This study aimed to compare the between-session reliability of different performance variables during 2 variants of the Smith machine back-squat exercise. Twenty-six male wrestlers performed 5 testing sessions (a 1-repetition maximum [1RM] session, and 4 experimental sessions [2 with the pause and 2 with the rebound technique]). Each experimental session consisted of performing 3 repetitions against 5 loads (45–55–65–75–85% of the 1RM). Mean velocity (MV), mean power (MP), peak velocity (PV), and peak power (PP) variables were recorded by a linear position transducer (GymAware PowerTool). The best and average scores of the 3 repetitions were considered for statistical analyses. The coefficient of variation (CV) ranged from 3.89% (best PV score at 55% 1 RM using the pause technique) to 10.29% (average PP score at 85% 1 RM using the rebound technique). PP showed a lower reliability than MV, MP, and PV (CVratio ≥ 1.26). The reliability was comparable between the exercise techniques (CVratio = 1.08) and between the best and average scores (CVratio = 1.04). These results discourage the use of PP to assess back-squat performance at submaximal loads. The remaining variables (MV, MP, or PV), exercise techniques (pause or rebound), and repetition criteria (best score or average score) can be indistinctly used due to their acceptable and comparable reliability.
Stakeholder Perspectives to Support Graphical User Interface Design for Children with Autism Spectrum Disorder: A Qualitative Study
Authors: Groba Betania , Nieto-Riveiro Laura , Canosa Nereida , Concheiro-Moscoso Patricia , Miranda-Duro María del Carmen , Pereira Javier
Published: April 2021

The development of digital supports for people with autism has increased considerably in recent years. Technology designers and developers have interpreted the needs and learning styles of people with autism in different ways. As a result, there are generic, non-specific or heterogeneous guidelines for the design and development of technology for people with autism. This study aims to identify and describe the recommended elements to support graphical user interface design for children with Autism Spectrum Disorder (ASD), considering the stakeholders’ perspective, engaged in a computer application development. A qualitative, longitudinal, multicentre study was carried out. A sample of 39 participants belonging to four groups of stakeholders participated: children with autism, family members, professionals with experience in the intervention with children with autism, and professionals with expertise in the design and development of assistive technology. The techniques used to formalise the collection of information from participants were semi-structured interviews and observation. MAXQDA 2020 software (Verbi Software, Berlin, Germany) was used to analyse the data. The result is a guide with suggestions to support an interface design that emerges from the stakeholder perspectives. This study provides useful information to offer alternatives for children with ASD and facilitate the understanding of daily life.
Dysregulation of the Renin-Angiotensin-Aldosterone System (RAA) in Patients Infected with SARS-CoV-2-Possible Clinical Consequences
Authors: Dettlaff-Pokora Agnieszka , Swierczynski Julian
Published: April 2021

SARS-CoV-2 impairs the renin-angiotensin-aledosterone system via binding ACE2 enzyme. ACE2 plays a key role in the biosynthesis of angiotensin (1-7), catalyzing the conversion of angiotensin 2 into angiotensin (1-7) and the reaction of angiotensin synthesis (1-9), from which angiotensin is (1-7) produced under the influence of ACE (Angiotensin-Converting Enzyme). Angiotensin 2 is a potent vasoconstrictor and atherogenic molecule converted by ACE2 to reducing inflammation and vasodilating in action angiotensin (1-7). Angiotensin (1-9), that is a product of angiotensin 1 metabolism and precursor of angiotensin (1-7), also exerts cell protective properties. Balance between angiotensin 2 and angiotensin (1-7) regulates blood pressure and ACE2 plays a critical role in this balance. ACE2, unlike ACE, is not inhibited by ACE inhibitors at the doses used in humans during the treatment of arterial hypertension. Membrane ACE2 is one of the receptors that allows SARS-CoV-2 to enter the host cells. ACE2 after SARS-CoV-2 binding is internalized and degraded. Hence ACE2 activity on the cell surface is reduced leading to increase the concentration of angiotensin 2 and decrease the concentration of angiotensin (1-7). Disturbed angiotensins metabolism, changes in ratio between angiotensins with distinct biological activities leading to domination of atherogenic angiotensin 2 can increase the damage to the lungs.
Classification of Relaxation and Concentration Mental States with EEG
Authors: You Shingchern D.
Published: April 2021

In this paper, we study the use of EEG (Electroencephalography) to classify between concentrated and relaxed mental states. In the literature, most EEG recording systems are expensive, medical-graded devices. The expensive devices limit the availability in a consumer market. The EEG signals are obtained from a toy-grade EEG device with one channel of output data. The experiments are conducted in two runs, with 7 and 10 subjects, respectively. Each subject is asked to silently recite a five-digit number backwards given by the tester. The recorded EEG signals are converted to time-frequency representations by the software accompanying the device. A simple average is used to aggregate multiple spectral components into EEG bands, such as α, β, and γ bands. The chosen classifiers are SVM (support vector machine) and multi-layer feedforward network trained individually for each subject. Experimental results show that features, with α+β+γ bands and bandwidth 4 Hz, the average accuracy over all subjects in both runs can reach more than 80% and some subjects up to 90+% with the SVM classifier. The results suggest that a brain machine interface could be implemented based on the mental states of the user even with the use of a cheap EEG device.
A Semi-Automatic Semantic Consistency-Checking Method for Learning Ontology from Relational Database
Authors: Ma Chuangtao , Molnár Bálint , Benczúr András
Published: April 2021

To tackle the issues of semantic collision and inconsistencies between ontologies and the original data model while learning ontology from relational database (RDB), a semi-automatic semantic consistency checking method based on graph intermediate representation and model checking is presented. Initially, the W-Graph, as an intermediate model between databases and ontologies, was utilized to formalize the semantic correspondences between databases and ontologies, which were then transformed into the Kripke structure and eventually encoded with the SMV program. Meanwhile, description logics (DLs) were employed to formalize the semantic specifications of the learned ontologies, since the OWL DL showed good semantic compatibility and the DLs presented an excellent expressivity. Thereafter, the specifications were converted into a computer tree logic (CTL) formula to improve machine readability. Furthermore, the task of checking semantic consistency could be converted into a global model checking problem that could be solved automatically by the symbolic model checker. Moreover, an example is given to demonstrate the specific process of formalizing and checking the semantic consistency between learned ontologies and RDB, and a verification experiment was conducted to verify the feasibility of the presented method. The results showed that the presented method could correctly check and identify the different kinds of inconsistencies between learned ontologies and its original data model.
Exploring the Effects of Normative Beliefs toward Citizen Engagement on eParticipation Technologies
Authors: Shihab Muhammad Rifki , Hidayanto Achmad Nizar , Putra Panca Hadi
Published: April 2021

This research evaluates the effects of normative beliefs toward citizen engagement on eParticipation. Normative beliefs herein were assessed from the perspectives of citizenship norms, which include engaged-citizenship norms and duty-based norms, as well as the perspective of subjective norms, namely civic norms. A questionnaire was devised as the research instrument, and a survey was conducted as a means for data collection. The respondents were citizens who reside in the Greater Jakarta Region, in Indonesia, whom have had previous experiences with eParticipation. A total of 172 valid responses were collected in this study. Data were analyzed using Partial Least Squares Structural Equational Modeling (PLS-SEM), aided with SmartPLS 3 as a tool. The research results confirmed that perceived public value and perceived public satisfaction both concertedly shape citizens’ engagement in eParticipation. Furthermore, perceived public value as a pre-transactional norm also served as an antecedent to the post-transactional norm of perceived public satisfaction. The results also revealed that perceived public value was affected by a sole citizenship norm, namely, duty-based norm. Additionally, perceived public satisfaction was not affected by neither engaged-citizenship norm nor duty-based norm. Conversely, civic norms showed significant effects on both perceived public value and perceived public satisfaction.
Improving Contagion and Horizontal Transmission of Entomopathogenic Fungi by the White-Spotted Longicorn Beetle, Anoplophora malasiaca, with Help of Contact Sex Pheromone
Authors: Fujiwara-Tsujii Nao , Yasui Hiroe
Published: April 2021

The white-spotted longicorn beetle, Anoplophora malasiaca, is one of the most destructive pests of horticultural crops and street trees. Effective controls are needed because the effect of marketed insecticides is limited. Entomopathogenic fungi offer a solution, and improving the rate of infection would be a breakthrough in this beetle’s control. The combination of pathogenic fungi and the beetle’s contact sex pheromone was suggested. The surface of the female body is covered with contact sex pheromone, which elicit male mating behavior. To develop a method for the practical control of this beetle, we evaluated the arrestant activity of female extract containing contact pheromone coated on a black glass model. Males presented with a coated model held on for 5 h (mean) during an 8-h experiment. In contrast, males presented with a control model held on for <0.3 h. Males that held onto coated models attached to fabric impregnated with conidia of the fungus Beauveria brongniartii picked up much conidia, which they then passed on to females during mating.
Variants in the Mitochondrial Genome Sequence of Rhyzopertha dominica (Fabricius) (Coleoptera: Bostrycidae)
Authors: Perkin Lindsey C. , Smith Timothy P. L. , Oppert Brenda
Published: April 2021

The lesser grain borer, Rhyzopertha dominica, is a coleopteran pest of stored grains and is mainly controlled by phosphine fumigation, but the increase in phosphine-resistant populations threatens efficacy. Some phosphine-resistant insects have reduced respiration, and thus studying the mitochondrial genome may provide additional information regarding resistance. Genomic DNA from an inbred laboratory strain of R. dominica was extracted and sequenced with both short (Illumina) and long (Pacific Biosciences) read technologies for whole genome sequence assembly and annotation. Short read sequences were assembled and annotated by open software to identify mitochondrial sequences, and the assembled sequence was manually annotated and verified by long read sequences. The mitochondrial genome sequence for R. dominica had a total length of 15,724 bp and encoded 22 trna genes, 2 rRNA genes, 13 protein coding genes (7 nad subunits, 3 cox, 2 atp, and 1 cytB), flanked by a long control region. We compared our predicted mitochondrial genome to that of another from a R. dominica strain from Jingziguan (China). While there was mostly agreement between the two assemblies, key differences will be further examined to determine if mutations in populations are related to insecticide control pressure, mainly that of phosphine. Differences in sequence data, assembly, and annotation also may result in different genome interpretations.
Forgiveness Interventions for Older Adults: A Review
Authors: López Javier , Serrano Maria Inés , Giménez Isabel , Noriega Cristina
Published: April 2021

A meta-analysis of the efficacy of forgiveness interventions in older adults was conducted. International databases (Medline, PsycINFO, Scopus, Web of Science) were searched for studies published from 1990 to 2020 that attempted to promote forgiveness in older adults. Most intervention studies are group treatments targeted towards community-dwelling older adults. Participants in these studies are mainly women. The intervention objectives and contents vary widely and often criteria are not well-defined. Participants that received forgiveness interventions reported significantly higher levels of forgiveness than participants that did not receive treatment. Additionally, forgiveness interventions resulted in more changes in depression, stress and anger than no intervention conditions. Forgiveness treatment also enhances positive states (satisfaction with life, subjective happiness, and psychological wellbeing). The reported effects are moderate. The specific treatment model (e.g., Enright’s, Worthington’s) and format (e.g., group-based interventions and individually delivered programs) do not differentially predict better outcomes. In conclusion, future intervention studies should include more male participants and utilize a broader range of follow-up periods. Caution must be exercised because of the limited number of studies developed to date; researchers must be cautious when generalizing the results.
Active Infective Native and Prosthetic Valve Endocarditis: Short- and Long-Term Outcomes of Patients after Surgical Treatment
Authors: Salem Mohamed , Friedrich Christine , Saad Mohammed , Frank Derk , Salem Mostafa , Puehler Thomas , Schoettler Jan , Schoeneich Felix , Cremer Jochen , Haneya Assad
Published: April 2021

Background: Active infective endocarditis (IE) is a serious disease associated with high mortality. The current study represents our experience over 18 years with surgical treatment for active infective native and prosthetic valve endocarditis (INVE, IPVE). Method: Analysis of 413 patients (171 with IPVE vs. 242 with INVE) who underwent cardiac surgery due to IE between 2002 and 2020. Results: Patients with IPVE were significantly older (64.9 ± 13.2 years vs. 58.3 ± 15.5 years; p < 0.001) with higher EuroSCORE II (21.2 (12.7; 41.8) vs. 6.9 (3.0; 17.0); p < 0.001)) and coronary heart disease (50.6% vs. 38.0%; p < 0.011). Preoperative embolization was significantly higher within INVE (35.5% vs. 16.4%; p < 0.001) with high incidence of cerebral embolization (18.6% vs. 7.6%; p = 0.001) and underwent emergency curative surgery than the IPVE group (19.6% vs. 10.6%; p < 0.001). However, patients with IPVE were significantly represented with intracardiac abscess (44.4% vs.15.7%; p < 0.001). Intraoperatively, the duration of surgery was expectedly significantly higher in the IPVE group (356 min vs. 244 min.; p = 0.001) as well as transfusion of blood (4 units (0–27) vs. 2 units (0–14); p < 0.001). Post-operatively, the incidence of bleeding was markedly higher within the IPVE group (700 mL (438; 1163) vs. 500 mL (250; 1075); p = 0.005). IPVE required significantly more permanent pacemakers (17.6% vs. 7.5%: p = 0.002). The 30-day mortality was higher in the IPVE group (24.6% vs. 13.2%; p < 0.003). Conclusion: Patients with INVE suffered from a higher incidence of cerebral embolization and neurological deficits than patients with IPVE. Surgical treatment in INVE is performed mostly as an emergency indication. However, patients with IPVE were represented commonly with intracardiac abscess, and had a higher indication of pacemaker implantation. The short- and long-term mortality rate among those patients was still high.
Low Hypoperfusion Intensity Ratio Is Associated with a Favorable Outcome Even in Large Ischemic Core and Delayed Recanalization Time
Authors: Baek Jang-Hyun , Kim Young Dae , Lee Ki Jeong , Choi Jin Kyo , Baik Minyoul , Kim Byung Moon , Kim Dong Joon , Heo Ji Hoe , Nam Hyo Suk
Published: April 2021

In ischemic brain tissue, hypoperfusion severity can be assessed using the hypoperfusion intensity ratio (HIR). We evaluated the link between HIR and clinical outcomes after successful recanalization by endovascular treatment. We retrospectively reviewed 162 consecutive patients who underwent endovascular treatment for intracranial large vessel occlusion. The HIR was calculated using an automated software program, with initial computed tomography perfusion images. The HIR was compared between patients with and without favorable outcomes. To observe the modifying effect of the HIR on the well-known major outcome determinants, regression analyses were performed in the low and high HIR groups. The median HIR value was significantly lower in patients with a favorable outcome, with an optimal cut-off point of 0.54. The HIR was an independent factor for a favorable outcome in a specific multivariable model and was significantly correlated with the Alberta Stroke Program Early Computed Tomography Score (ASPECTS). In contrast to the high HIR group, the low HIR group showed that ASPECTS and onset-to-recanalization time were not independently associated with a favorable outcome. Finally, the low HIR group had a more favorable outcome even in cases with an unfavorable ASPECTS and onset-to-recanalization time. The HIR could be useful in predicting outcomes after successful recanalization.
A New Outlook on the Ability to Accumulate an Iodine Contrast Agent in Solid Lung Tumors Based on Virtual Monochromatic Images in Dual Energy Computed Tomography (DECT): Analysis in Two Phases of Contrast Enhancement
Authors: Zegadło Arkadiusz , Żabicka Magdalena , Różyk Aleksandra , Więsik-Szewczyk Ewa
Published: April 2021

For some time, dual energy computed tomography (DECT) has been an established method used in a vast array of clinical applications, including lung nodule assessment. The aim of this study was to analyze (using monochromatic DECT images) how the X-ray absorption of solitary pulmonary nodules (SPNs) depends on the iodine contrast agent and when X-ray absorption is no longer dependent on the accumulated contrast agent. Sixty-six patients with diagnosed solid lung tumors underwent DECT scans in the late arterial phase (AP) and venous phase (VP) between January 2017 and June 2018. Statistically significant correlations (p ≤ 0.001) of the iodine contrast concentration were found in the energy range of 40–90 keV in the AP phase and in the range of 40–80 keV in the VP phase. The strongest correlation was found between the concentrations of the contrast agent and the scanning energy of 40 keV. At the higher scanning energy, no significant correlations were found. We concluded that it is most useful to evaluate lung lesions in DECT virtual monochromatic images (VMIs) in the energy range of 40–80 keV. We recommend assessing SPNs in only one phase of contrast enhancement to reduce the absorbed radiation dose.
Performance of Fatty Liver Index in Identifying Non-Alcoholic Fatty Liver Disease in Population Studies. A Meta-Analysis
Authors: Castellana Marco , Donghia Rossella , Guerra Vito , Procino Filippo , Lampignano Luisa , Castellana Fabio , Zupo Roberta , Sardone Rodolfo , De Pergola Giovanni , Romanelli Francesco , Trimboli Pierpaolo , Giannelli Gianluigi
Published: April 2021

Background. Fatty liver index (FLI) is a non-invasive tool used to stratify the risk of non-alcoholic fatty liver disease (NAFLD) in population studies; whether it can be used to exclude or diagnose this disorder is unclear. We conducted a meta-analysis to assess the prevalence of NAFLD in each FLI class and the performance of FLI in detecting NAFLD. Methods. Four databases were searched until January 2021 (CRD42021231367). Original articles included were those reporting the performance of FLI and adopting ultrasound, computed tomography, or magnetic resonance as a reference standard. The numbers of subjects with NAFLD in FLI classes <30, 30–60, and ≥60, and the numbers of subjects classified as true/false positive/negative when adopting 30 and 60 as cut-offs were extracted. A random-effects model was used for pooling data. Results. Ten studies were included, evaluating 27,221 subjects without secondary causes of fatty liver disease. The prevalence of NAFLD in the three FLI classes was 14%, 42%, and 67%. Sensitivity, specificity, positive predictive value, negative predictive value, likelihood ratio for positive results, likelihood ratio for negative results, and diagnostic odds ratio were 81%, 65%, 53%, 84%, 2.3, 0.3, and 7.8 for the lower cut-off and 44%, 90%, 67%, 76%, 4.3, 0.6, and 7.3 for the higher cut-off, respectively. A similar performance was generally found in studies adopting ultrasound versus other imaging modalities. Conclusions. FLI showed an adequate performance in stratifying the risk of NAFLD. However, it showed only weak evidence of a discriminatory performance in excluding or diagnosing this disorder.
Autoinflammatory Features in Gouty Arthritis
Authors: Galozzi Paola , Bindoli Sara , Doria Andrea , Oliviero Francesca , Sfriso Paolo
Published: April 2021

In the panorama of inflammatory arthritis, gout is the most common and studied disease. It is known that hyperuricemia and monosodium urate (MSU) crystal-induced inflammation provoke crystal deposits in joints. However, since hyperuricemia alone is not sufficient to develop gout, molecular-genetic contributions are necessary to better clinically frame the disease. Herein, we review the autoinflammatory features of gout, from clinical challenges and differential diagnosis, to the autoinflammatory mechanisms, providing also emerging therapeutic options available for targeting the main inflammatory pathways involved in gout pathogenesis. This has important implication as treating the autoinflammatory aspects and not only the dysmetabolic side of gout may provide an effective and safer alternative for patients even in the prevention of possible gouty attacks.
Tinnitus and Neuropsychological Dysfunction in the Elderly: A Systematic Review on Possible Links
Authors: Malesci Rita , Brigato Francesca , Di Cesare Tiziana , Del Vecchio Valeria , Laria Carla , De Corso Eugenio , Fetoni Anna
Published: April 2021

Introduction: Tinnitus is a common and disabling symptom often associated with hearing loss. While clinical practice frequently shows that a certain degree of psychological discomfort often characterizes tinnitus suffers, it has been recently suggested in adults as a determining factor for cognitive decline affecting attention and memory domains. The aim of our systematic review was to provide evidence for a link between tinnitus, psychological distress, and cognitive dysfunction in older patients and to focus on putative mechanisms of this relationship. Methods: We performed a systematic review, finally including 192 articles that were screened. This resulted in 12 manuscripts of which the full texts were included in a qualitative analysis. Results: The association between tinnitus and psychological distress, mainly depression, has been demonstrated in older patients, although only few studies addressed the aged population. Limited studies on cognitive dysfunction in aged patients affected by chronic tinnitus are hardly comparable, as they use different methods to validate cognitive impairment. Actual evidence does not allow us with certainty to establish if tinnitus matters as an independent risk factor for cognitive impairment or evolution to dementia. Conclusion: Tinnitus, which is usually associated with age-related hearing loss, might negatively affect emotional wellbeing and cognitive capacities in older people, but further studies are required to improve the evidence.
The Association between Physical Activity and CAMDEX-DS Changes Prior to the Onset of Alzheimer’s Disease in Down Syndrome
Authors: Pape Sarah E. , Baksh R. Asaad , Startin Carla , Hamburg Sarah , Hithersay Rosalyn , Strydom Andre
Published: April 2021

Background: People with Down syndrome are at ultra-high risk of developing Alzheimer’s dementia. At present, there are no preventative or curative treatments. Evidence from sporadic Alzheimer’s disease literature suggests that lifestyle factors including physical activity may help maintain cognitive and functional skills and reduce dementia risk. Our study aimed to explore the association between regular exercise undertaken by participants with Down syndrome and changes in dementia-related domains of cognition and function. This was to consider whether physical activity may be a protective measure to delay cognitive decline and dementia in Down syndrome. Methods: Demographic, lifestyle, and health information was collected at baseline and at a two year follow up from 214 adults with Down syndrome without dementia, who also underwent assessment using the Cambridge Examination for Mental Disorders of Older People with Down Syndrome and Others with Intellectual Disabilities (CAMDEX-DS) and genetic analysis. Logistic regression models were used to examine the potential associations between decline in CAMDEX-DS domains and exercise whilst controlling for key variables. Results: At baseline, engaging in moderate intensity exercise was associated with a 47% reduced risk of everyday skills decline and engaging in high intensity exercise was associated with a 62% reduced risk of decline in personality and behaviour. At follow-up, high levels of exercise were associated with an 87% reduced risk of decline in personality and behaviour. Moderate intensity exercise at baseline was associated with a 62% reduction in risk of decline during the follow-up period in memory and orientation. Discussion: Based on our data it appears that regular moderate and high intensity exercise could reduce the risk of clinically detectable decline in a Down syndrome population with possible long-term benefits. People with Down syndrome may engage in less physical activity than their peers, and barriers remain which can prevent people with Down syndrome engaging in exercise. Our work highlights how important it is that people with Down syndrome are supported to be physically active, and to promote exercise as part of a healthy ageing plan. Clinical trials in this area would be justified to determine if engaging in exercise can lead to realistic improvements in maintaining functioning and delaying dementia onset in Down syndrome and to help develop guidance in this area.
Parathyroid Hormone Secretion and Receptor Expression Determine the Age-Related Degree of Osteogenic Differentiation in Dental Pulp Stem Cells
Authors: Bhandi Shilpa , Alkahtani Ahmed , Reda Rodolfo , Mashyakhy Mohammed , Boreak Nezar , Maganur Prabhadevi C. , Vishwanathaiah Satish , Mehta Deepak , Vyas Nishant , Patil Vikrant , Raj A. Thirumal , Testarelli Luca , Patil Shankargouda
Published: April 2021

Objective: To demonstrate the levels of parathyroid hormone secretion and genetic expressions of parathyroid hormone (PTH) and PTH1 receptor (PTH1R) genes in the dental pulp stem cells (DPSCs) from different age groups before and after induction of osteogenic differentiation. In addition, we also wanted to check their correlation with the degree of osteogenic differentiation. Methods: Human primary DPSCs from three age groups (milk tooth (SHEDs), 7–12 years old; young DPSCs (yDPSCs), 20–40 years old; old DPSCs (oDPSCs), 60+ years old) were characterized for mesenchymal stem cell (MSC) markers. DPSCs were subjected to osteogenic differentiation and functional staining. Gene expression levels were analyzed by qRT-PCR. Surface receptor analysis was done by flow cytometry. Comparative protein levels were evaluated by ELISA. Results: All SHEDs, yDPSCs, and oDPSCs were found to be expressing mesenchymal stem cell markers. SHEDs showed more mineralization than yDPSCs and oDPSCs after osteogenic induction. SHEDs exhibited higher expression of PTH and PTH1R before and after osteogenic induction, and after osteogenic induction, SHEDs showed more expression for RUNX2, ALPL, and OCN. Higher levels of PTH were observed in SHEDs and yDPSCs, and the number of PTH1R positive cells was relatively lower in yDPSCs and oDPSCs than in SHEDs. After osteogenic induction, SHEDs were superior in the secretion of OPG, and the secretions of ALPL and PTH and the number of PTH1R positive cells were relatively low in the oDPSCs. Conclusions: The therapeutic quality of dental pulp stem cells is largely based on their ability to retain their stemness characteristics. This study emphasizes the criterion of aging, which affects the secretion of PTH by these cells, which in turn attenuates their osteogenic potential.
An Error Analysis of the CN Weighed DG θ Method of the Convection Equation
Authors: Hossain Muhammad Shakhawat , Xiong Chunguang
Published: April 2021

In this research paper, a weighed DG finite element method is proposed for solving convection equations with an easy execution and analysis. The key aim of this method is to design an error estimation for space and time of a discontinuous approximation on general finite element meshes. The efficiency of the parameter θ in the order of convergence of the solutions is also exposed. Some numerical examples were tested that demonstrated the strength and flexibility of the method.
Forgotten Motivational Factors of Boyacense Colombian Entrepreneurs: A Subjective Analysis of Second-Generation Incidences
Authors: Blanco-Mesa Fabio , Leon-Castro Ernesto , Bermudez-Mondragon Dario , Castro-Amado Mayra
Published: April 2021

The main aim of this article was, using subjective information, to analyze the forgotten factors that influence the motivation of Boyacenses to become entrepreneurs. The study was carried out with entrepreneurs enrolled in the Boyacá Region Brand Program (BRBP). For the treatment of the collected data, the method of the experts and the theory of forgotten effects were used to identify second-generation incidences involving motivational factors in Boyacense entrepreneurs. The research questions that guided the study were as follows: What are the forgotten effects among the motivational factors of entrepreneurship? What are the intermediate factors that generate the second-order effects? What are the economic, psychological, cultural, and environmental factors that have second-order effects among them? Among the main findings, it was possible to visualize second-degree relationships between economic, cultural, and psychological factors. Additionally, in the case of entrepreneurs from Boyacá, the primary motivation for most of them to develop their business idea was economic independence, while the least motivating was the improvement of social status. Likewise, the need for achievement was highlighted. Finally, the country’s economic situation was found to work against the development of entrepreneurship.
Bioactive Compounds from Marine Sponges: Fundamentals and Applications
Authors: Varijakzhan Disha , Loh Jiun-Yan , Yap Wai-Sum , Yusoff Khatijah , Seboussi Rabiha , Lim Swee-Hua Erin , Lai Kok-Song , Chong Chou-Min
Published: April 2021

Marine sponges are sessile invertebrates that can be found in temperate, polar and tropical regions. They are known to be major contributors of bioactive compounds, which are discovered in and extracted from the marine environment. The compounds extracted from these sponges are known to exhibit various bioactivities, such as antimicrobial, antitumor and general cytotoxicity. For example, various compounds isolated from Theonella swinhoei have showcased various bioactivities, such as those that are antibacterial, antiviral and antifungal. In this review, we discuss bioactive compounds that have been identified from marine sponges that showcase the ability to act as antibacterial, antiviral, anti-malarial and antifungal agents against human pathogens and fish pathogens in the aquaculture industry. Moreover, the application of such compounds as antimicrobial agents in other veterinary commodities, such as poultry, cattle farming and domesticated cats, is discussed, along with a brief discussion regarding the mode of action of these compounds on the targeted sites in various pathogens. The bioactivity of the compounds discussed in this review is focused mainly on compounds that have been identified between 2000 and 2020 and includes the novel compounds discovered from 2018 to 2021.
Catalytic Reduction of Graphene Oxide Membranes and Water Selective Channel Formation in Water–Alcohol Separations
Authors: Zang Yushi , Peek Alex , Shin Yongsoon , Gotthold David , Hinds Bruce J.
Published: April 2021

Graphene oxide (GO) is a promising membrane system for chemical separation applications due to its 2-D nanofluidics properties and an ability to control interplanar spacing for selectivity. The permeance of water, methanol (MeOH) and isopropyl alcohol (IPA) through 5 µm thick membranes was found to be 0.38 ± 0.15, 0.33 ± 0.16 and 0.42 ± 0.31 LMH/bar (liter/m2·h·bar), respectively. Interestingly, the permeance of a water–alcohol mixture was found to be dramatically lower (~0.01 LMH/bar) than any of its components. Upon removing the solvent mixture, the transmembrane flux of the pure solvent was recovered to near the original permeance. The interlayer space of a dried GO membrane was found to be 8.52 Å, which increased to 12.19 Å. 13.26 Å and 16.20 Å upon addition of water, MeOH and IPA. A decrease in d-space, about 2 Å, was consistently observed when adding alcohol to water wetted GO membrane and an optical color change and reduction in permeance. A newly proposed mechanism of a partial reduction of GO through a catalytic reaction with the water–alcohol mixture is consistent with experimental observations.
3D-Printed Connector for Revision Limb Salvage Surgery in Long Bones Previously Using Customized Implants
Authors: Park Jong-Woong , Kang Hyun-Guy , Kim June-Hyuk , Kim Han-Soo
Published: April 2021

In orthopedic oncology, revisional surgery due to mechanical failure or local recurrence is not uncommon following limb salvage surgery using an endoprosthesis. However, due to the lack of clinical experience in limb salvage surgery using 3D-printed custom-made implants, there have been no reports of revision limb salvage surgery using a 3D-printed implant. Herein, we present two cases of representative revision limb salvage surgeries that utilized another 3D-printed custom-made implant while retaining the previous 3D-printed custom-made implant. A 3D-printed connector implant was used to connect the previous 3D-printed implant to the proximal ulna of a 40-year-old man and to the femur of a 69-year-old woman. The connector bodies for the two junctions of the previous implant and the remaining host bone were designed for the most functional position or angle by twisting or tilting. Using the previous 3D-printed implant as a taper, the 3D-printed connector was used to encase the outside of the previous implant. The gap between the previous implant and the new one was subsequently filled with bone cement. For both the upper and lower extremities, the 3D-printed connector showed stable reconstruction and excellent functional outcomes (Musculoskeletal Tumor Society scores of 87% and 100%, respectively) in the short-term follow-up. To retain the previous 3D-printed implant during revision limb salvage surgery, an additional 3D-printed implant may be a feasible surgical option.