bluuebunny/crossref_metadata_2025 huggingface dataset:
Prevention and education evidence review: Gambling-related harm
Authors: Hilbrecht Margo , Gainsbury Sally M. , Tabri Nassim , Wohl Michael J. A. , Xuereb Silas , Derevensky Jeffrey L. , Rodda Simone N. , Sheila McKnight , Jess Voll , Brittany Gottvald
Published: September 2021

This report supports an evidence-based approach to the prevention and education objective of the National Strategy to Reduce Harm from Gambling. Applying a public health policy lens, it considers three levels of measures: universal (for the benefit of the whole population), selective (for the benefit of at-risk groups), and indicated (for the benefit of at-risk individuals). Six measures are reviewed by drawing upon a range of evidence in the academic and grey literature. The universal level measures are “Regulatory restriction on how gambling is provided” and “Population-based safer gambling/responsible gambling efforts.” Selective measures focus on age cohorts in a chapter entitled, “Targeted safer gambling campaigns for children, youth, and older adults.” The indicated measures are “Brief internet delivered interventions for gambling,” “Systems and tools that produced actual (‘hard’) barriers and limit access to funds,” and “Self-exclusion.” Since the quantity and quality of the evidence base varied by measure, appropriate review methods were selected to assess publications using a systematic, scoping, or narrative approach. Some measures offered consistent findings regarding the effectiveness of interventions and initiatives, while others were less clear. Unintended consequences were noted since it is important to be aware of unanticipated, negative consequences resulting from prevention and education activities. After reviewing the evidence, authors identified knowledge gaps that require further research, and provided guidance for how the findings could be used to enhance the prevention and education objective. The research evidence is supplemented by consultations with third sector charity representatives who design and implement gambling harm prevention and education programmes. Their insights and experiences enhance, support, or challenge the academic evidence base, and are shared in a separate chapter. Overall, research evidence is limited for many of the measures. Quality assessments suggest that improvements are needed to support policy decisions more fully. Still, opportunities exist to advance evidence-based policy for an effective gambling harm prevention and education plan.
PROSPECTS OF HYDROGEN USE AS MOTOR FUEL FOR TRANSPORT VEHICLES
Authors: Goritsky Volodymyr , Kovalov Serhii , Zakrevsky Oleksandr
Published: September 2021

It is considered some prospects on the use of hydrogen as fuel for motor vehicles. Institutional, scientific and technological preconditions which facilitate enlarged application of hydrogen fuel are argued. It is briefly described the principal directions of theoretical investigation and also experimental works in this area.
ESTABLISHMENT OF A UNIFORM ROUTE GUIDANCE SYSTEM IN UKRAINE TAKING INTO ACCOUNT THE WORLD’S BEST PRACTICES TO IMPROVE ROAD SAFETY
Authors: Nagrebelna Liydmila , Kononenko Alla
Published: September 2021

Route guidance tools have a significant impact on road safety on public roads, as they carry important information on route guidance: provide drivers with information on the location on a specified road, regularly confirm the planned direction of movement, timely inform road users about the change of specified route direction and indicate the distance to the final object. This is very important information for the driver and it is difficult to overestimate it in road safety. Timely informing of drivers on the route direction will allow them carrying out adjustments in advance and feel confident on the road. Ensuring the completeness of route guidance information prevents emergency braking of vehicles in the area of traffic junctions and violation of the maneuvering rules to make a turn / turnaround in the right direction, and such maneuvers lead to road accidents. The establishment of a uniform route guidance system will reduce the road accident level and the cost of transportation due to excessive mileage of vehicles in the case of wrong route and help the drivers freely and easily get to their destination. The main need for information is felt by the drivers of vehicles whose actions directly impact the road safety. This, in turn, determines the basic principles and requirements for the composition, placement and choice of information tools. During the trip, the drivers need the information that would allow them navigating freely on the roads when passing the selected route that will relieve the stress of drivers and reduce the probability of road accidents, as well as increase the capacity of roads. It helps the drivers to adjust the previously selected route taking into account the real traffic conditions during the trip thereby minimizing the time consumption. To navigate the roads during movement, the drivers need the information about streets, objects and directions. Such information is provided by information signs, with which the road network must be equipped in sufficient quantity. The information provision should differ depending on the settlements, public roads and traffic conditions. A comprehensive solution of the issue of quality information provision to the road users can be achieved only by establishing a uniform route guidance system for road users which will take into account the needs for information of different categories of road users.
Morphology and Mechanical Properties of Plantar Fascia in Flexible Flatfoot: A Noninvasive In Vivo Study
Authors: Qian Zhihui , Jiang Zhende , Wu Jianan , Chang Fei , Liu Jing , Ren Lei , Ren Luquan
Published: September 2021

Plantar fascia plays an important role in human foot biomechanics; however, the morphology and mechanical properties of plantar fascia in patients with flexible flatfoot are unknown. In this study, 15 flexible flatfeet were studied, each plantar fascia was divided into 12 positions, and the morphologies and mechanical properties in the 12 positions were measured in vivo with B-mode ultrasound and shear wave elastography (SWE). Peak pressures under the first to fifth metatarsal heads (MH) were measured with FreeStep. Statistical analysis included 95% confidence interval, intragroup correlation coefficient (ICC1,1), one-way analysis of variance (one-way ANOVA), and least significant difference. The results showed that thickness and Young’s modulus of plantar fascia were the largest at the proximal fascia (PF) and decreased gradually from the proximal end to the distal end. Among the five distal branches (DB) of the fascia, the thickness and Young’s modulus of the second and third DB were larger. The peak pressures were also higher under the second and third MH. This study found a gradient distribution in that the thickness and Young’s modulus gradient decreased from the proximal end to the distal end of plantar fascia in the longitudinal arch of flexible flatfeet. In the transverse arch, the thickness and Young’s modulus under the second and third DB were larger than those under the other three DB in flexible flatfoot, and the peak pressures under the second and third MH were also larger than those under the other three MH in patients with flexible flatfoot. These findings deepen our understanding of the changes of biomechanical properties and may be meaningful for the study of pathological mechanisms and therapy for flexible flatfoot.
Roles and Cellular Localization of GBP2 and NAB2 During the Blood Stage of Malaria Parasites
Authors: Niikura Mamoru , Fukutomi Toshiyuki , Mitobe Jiro , Kobayashi Fumie
Published: September 2021

The quality control and export of mRNA by RNA-binding proteins are necessary for the survival of malaria parasites, which have complex life cycles. Nuclear poly(A) binding protein 2 (NAB2), THO complex subunit 4 (THO4), nucleolar protein 3 (NPL3), G-strand binding protein 2 (GBP2) and serine/arginine-rich splicing factor 1 (SR1) are involved in nuclear mRNA export in malaria parasites. However, their roles in asexual and sexual development, and in cellular localization, are not fully understood. In this study using the rodent malaria parasite, Plasmodium berghei, we found that NAB2 and SR1, but not THO4, NPL3 or GBP2, played essential roles in the asexual development of malaria parasites. By contrast, GBP2 but not NPL3 was involved in male and female gametocyte production. THO4 was involved in female gametocyte production, but had a lower impact than GBP2. In this study, we focused on GBP2 and NAB2, which play important roles in the sexual and asexual development of malaria parasites, respectively, and examined their cellular localization. GBP2 localized to both the nucleus and cytoplasm of malaria parasites. Using immunoprecipitation coupled to mass spectrometry (IP-MS), GBP2 interacted with the proteins ALBA4, DOZI, and CITH, which play roles in translational repression. IP-MS also revealed that phosphorylated adapter RNA export protein (PHAX) domain-containing protein, an adaptor protein for exportin-1, also interacted with GBP2, implying that mRNA export occurs via the PHAX domain-containing protein pathway in malaria parasites. Live-cell fluorescence imaging revealed that NAB2 localized at the nuclear periphery. Moreover, IP-MS indicated that NAB2 interacted with transportin. RNA immunoprecipitation coupled to RNA sequencing revealed that NAB2 bound directly to 143 mRNAs, including those encoding 40S and 60S ribosomal proteins. Our findings imply that malaria parasites use an evolutionarily ancient mechanism conserved throughout eukaryotic evolution.
Assessment of Future Risks of Seasonal Municipal Water Shortages Across North America
Authors: Janssen Joseph , Radić Valentina , Ameli Ali
Published: September 2021

While anthropogenic climate change poses a risk to freshwater resources across the globe through increases in evapotranspiration and temperature, it is essential to quantify the risks at local scales in response to projected trends in both freshwater supply and demand. In this study, we use empirical modeling to estimate the risks of municipal water shortages across North America by assessing the effects of climate change on streamflow and urban water demand. In addition, we aim to quantify uncertainties in both supply and demand predictions. Using streamflow data from both the US and Canada, we first cluster 4,290 streamflow gauges based on hydrograph similarity and geographical location. We develop a set of multiple linear regression (MLR) models, as a simplified analog to a distributed hydrological model, with minimum input data requirements. These MLR models are calibrated to simulate streamflow for the 1993–2012 period using the ERA5 climate reanalysis data. The models are then used to predict streamflow for the 2080–2099 period in response to two climate scenarios (RCP4.5 and RCP8.5) from five global climate models. Another set of MLR models are constructed to project seasonal changes in municipal water consumption for the clustered domains. The models are calibrated against collected data on urban water use from 47 cities across the study region. For both streamflow and water use, we quantified uncertainties in our predictions using stochastic weather generators and Monte Carlo methods. Our study shows the strong predictive power of the MLR models for simulating both streamflow regimes (Kling-Gupta efficiency >0.5) and urban water use (correlation coefficient ≈0.7) in most regions. Under the RCP4.5 (RCP8.5) emissions scenario, the West Coast, the Southwest, and the Deep South (South-Central US and the Deep South) have the highest risk of municipal water shortages. Across the whole domain, the risk is the highest in the summer season when demand is high. We find that the uncertainty in projected changes to the water demand is substantially lower than the uncertainty in the projected changes to the supply. Regions with the highest risk of water shortages should begin to investigate mitigation and adaptation strategies.
The Role of Lower Crustal Rheology in Lithospheric Delamination During Orogeny
Authors: Chen Lin
Published: September 2021

The continental lower crust is an important composition- and strength-jump layer in the lithosphere. Laboratory studies show its strength varies greatly due to a wide variety of composition. How the lower crust rheology influences the collisional orogeny remains poorly understood. Here I investigate the role of the lower crust rheology in the evolution of an orogen subject to horizontal shortening using 2D numerical models. A range of lower crustal flow laws from laboratory studies are tested to examine their effects on the styles of the accommodation of convergence. Three distinct styles are observed: 1) downwelling and subsequent delamination of orogen lithosphere mantle as a coherent slab; 2) localized thickening of orogen lithosphere; and 3) underthrusting of peripheral strong lithospheres below the orogen. Delamination occurs only if the orogen lower crust rheology is represented by the weak end-member of flow laws. The delamination is followed by partial melting of the lower crust and punctuated surface uplift confined to the orogen central region. For a moderately or extremely strong orogen lower crust, topography highs only develop on both sides of the orogen. In the Tibetan plateau, the crust has been doubly thickened but the underlying mantle lithosphere is highly heterogeneous. I suggest that the subvertical high-velocity mantle structures, as observed in southern and western Tibet, may exemplify localized delamination of the mantle lithosphere due to rheological weakening of the Tibetan lower crust.
The Sick Adipose Tissue: New Insights Into Defective Signaling and Crosstalk With the Myocardium
Authors: Bermúdez Valmore , Durán Pablo , Rojas Edward , Díaz María P. , Rivas José , Nava Manuel , Chacín Maricarmen , Cabrera de Bravo Mayela , Carrasquero Rubén , Ponce Clímaco Cano , Górriz José Luis , D´Marco Luis
Published: September 2021

Adipose tissue (AT) biology is linked to cardiovascular health since obesity is associated with cardiovascular disease (CVD) and positively correlated with excessive visceral fat accumulation. AT signaling to myocardial cells through soluble factors known as adipokines, cardiokines, branched-chain amino acids and small molecules like microRNAs, undoubtedly influence myocardial cells and AT function via the endocrine-paracrine mechanisms of action. Unfortunately, abnormal total and visceral adiposity can alter this harmonious signaling network, resulting in tissue hypoxia and monocyte/macrophage adipose infiltration occurring alongside expanded intra-abdominal and epicardial fat depots seen in the human obese phenotype. These processes promote an abnormal adipocyte proteomic reprogramming, whereby these cells become a source of abnormal signals, affecting vascular and myocardial tissues, leading to meta-inflammation, atrial fibrillation, coronary artery disease, heart hypertrophy, heart failure and myocardial infarction. This review first discusses the pathophysiology and consequences of adipose tissue expansion, particularly their association with meta-inflammation and microbiota dysbiosis. We also explore the precise mechanisms involved in metabolic reprogramming in AT that represent plausible causative factors for CVD. Finally, we clarify how lifestyle changes could promote improvement in myocardiocyte function in the context of changes in AT proteomics and a better gut microbiome profile to develop effective, non-pharmacologic approaches to CVD.
The Negative Impacts of Acromegaly on Bone Microstructure Not Fully Reversible
Authors: Duan Lian , Yang Shengmin , Wang Lin Jie , Zhang Yuelun , Li Ran , Yang Hongbo , Zhao Yuxing , Du Hanze , Zhai Xiao , Gong Fengying , Pan Hui , Zhu Huijuan , Xia Weibo
Published: September 2021

Purpose

This study aimed to evaluate the bone turnover markers and bone microarchitecture parameters derived from high-resolution peripheral quantitative computed tomography (HR-pQCT) in active and controlled acromegaly patients.

Methods

This cross-sectional study involved 55 acromegaly patients from a tertiary hospital (23 males and 32 females, aged 45.0 ± 11.6 years). Firstly, growth hormone (GH), insulin-like growth factor-1 (IGF-1), and markers for bone turnover were assessed. Next, we derived peripheral bone microstructure parameters and volumetric bone mineral density (vBMD) through HR-pQCT. These parameters were compared between acromegaly patients and 110 healthy controls, as well as between 27 active and 28 controlled acromegaly patients. Moreover, the relationship between GH/IGF-1 and bone microstructure parameters was analyzed through multiple linear regression.

Results

As compared with healthy controls, acromegaly patients exhibited elevated cortical vBMD, reduced trabecular vBMD, and increased trabecular inhomogeneity in the distal radius and tibia. While controlled acromegaly patients had slower bone turnover, they did not necessarily have better bone microstructure relative to active patients in intergroup comparison. Nevertheless, multiple regression indicated that higher IGF-1 was associated with lower tibial stiffness and failure load. Additionally, males with higher IGF-1 typically had larger trabecular separation, lower trabecular number, and larger cortical pores in the radius. Moreover, patients with elevated GH typically had more porous cortical bone in the radius and fewer trabeculae in the tibia. However, the compromised bone strength in active patients was partially compensated by increased bone thickness. Furthermore, no significant linkage was observed between elevated GH/IGF-1 and the most important HR-pQCT parameters such as trabecular volumetric bone density.

Conclusion

Acromegaly adversely affected bone quality, even in controlled patients. As the deterioration in bone microstructure due to prolonged GH/IGF-1 exposure was not fully reversible, clinicians should be aware of the bone fragility of acromegaly patients even after they had achieved biochemical remission.
Gestational Hormone Concentrations Are Associated With Timing of Delivery in a Fetal Sex-Dependent Manner
Authors: Cathey Amber L. , Watkins Deborah J. , Rosario Zaira Y. , Vega Carmen M. Vélez , Mukherjee Bhramar , O’Neill Marie S. , Loch-Caruso Rita , Alshawabkeh Akram N. , Cordero José F. , Meeker John D.
Published: September 2021

Background

Early delivery remains a significant public health problem that has long-lasting impacts on mother and child. Understanding biological mechanisms underlying timing of labor, including endocrine disruption, can inform prevention efforts.

Methods

Gestational hormones were measured among 976 women in PROTECT, a longitudinal birth cohort in Puerto Rico. We evaluated associations between hormone concentrations at 18 and 26 weeks gestation and gestational age at birth, while assessing effect modification by fetal sex. Exploratory analyses assessed binary outcomes of overall preterm birth (PTB, <37 weeks gestation) and the spontaneous PTB subtype, defined as preterm premature rupture of membranes, spontaneous preterm labor, or both. Multivariable logistic and linear regressions were fit using visit-specific hormone concentrations, and fetal sex-specific effects were estimated using interaction terms. Main outcome models were adjusted for maternal age, education, marital status, alcohol consumption, environmental tobacco smoke exposure, and pre-pregnancy body mass index (BMI). Exploratory models adjusted for maternal age and education.

Results

We observed reduced gestational age at birth with higher circulating CRH (β: -2.73 days, 95% CI: -4.97, -0.42), progesterone (β: -4.90 days, 95% CI: -7.07, -2.73), and fT4 concentrations (β: -2.73 days, 95% CI: -4.76, -0.70) at 18 weeks specifically among male fetuses. Greater odds of overall and spontaneous PTB were observed among males with higher CRH, estriol, progesterone, total triiodothyronine (T3), and free thyroxine (fT4) concentrations. Greater odds of PTB among females was observed with higher testosterone concentrations.

Conclusions

Various associations between hormones and timing of delivery were modified by fetal sex and timing of hormone measurement. Future studies are needed to understand differential mechanisms involved with timing of labor between fetal sexes.
Investigation on Aerosol Pool Scrubbing Model During Severe Accidents
Authors: He L. W. , Li Y. X. , Zhou Y. , Chen S. , Tong L. L. , Cao X. W.
Published: September 2021

During a nuclear power plant severe accident, discharging gas mixture into the spent-fuel pool is an alternative containment depressurization measurement through which radioactive aerosols can be scrubbed. However, it is necessary to develop a code for analyzing the decontamination factor of aerosol pool scrubbing. This article has established the analysis model considering key aerosol pool scrubbing mechanisms and introduced the Akita bubble size relationship. In addition, a code for evaluating the decontamination factor of aerosol pool scrubbing was established. The Advanced Containment Experiment and Light Water Reactor Advanced Containment Experiment were simulated with the code considering different bubble sizes of the Akita model and MELCOR default value to verify the suitability of the Akita bubble size model for simulating aerosol pool scrubbing. Furthermore, the simulation results were compared with the results analyzed by MELCOR code and COCOSYS code from literature, and equivalent predictive ability was observed. In addition, a sensitivity analysis on bubble size was conducted, and the contribution of different behaviors and mechanisms has been discussed. Finally, the bubble breakup equation was revised and verified with the conditions of the multi-hole bubbler in the Advanced Containment Experiment and Light Water Reactor Advanced Containment Experiment.
Could β-Lactam Antibiotics Block Humoral Immunity?
Authors: Melenotte Cléa , Pontarotti Pierre , Pinault Lucile , Mège Jean-Louis , Devaux Christian , Raoult Didier
Published: September 2021

It has been reported that treatment with β-lactam antibiotics induces leukopenia and candidemia, worsens the clinical response to anticancer immunotherapy and decreases immune response to vaccination. β-lactamases can cleave β-lactam antibiotics by blocking their activity. Two distincts superfamilies of β-lactamases are described, the serine β-lactamases and the zinc ion dependent metallo-β-lactamases. In human, 18 metallo-β-lactamases encoding genes (hMBLs) have been identified. While the physiological role of most of them remains unknown, it is well established that the SNM1A, B and C proteins are involved in DNA repair. The SNM1C/Artemis protein is precisely associated in the V(D)J segments rearrangement, that leads to immunoglobulin (Ig) and T-cell receptor variable regions, which have a crucial role in the immune response. Thus in humans, SNM1C/Artemis mutation is associated with severe combined immunodeficiency characterized by hypogammaglobulinemia deficient cellular immunity and opportunistic infections. While catalytic site of hMBLs and especially that of the SNM1 family is highly conserved, in vitro studies showed that some β-lactam antibiotics, and precisely third generation of cephalosporin and ampicillin, inhibit the metallo-β-lactamase proteins SNM1A & B and the SNM1C/Artemis protein complex. By analogy, the question arises as to whether β-lactam antibiotics can block the SNM1C/Artemis protein in humans inducing transient immunodeficiency. We reviewed here the literature data supporting this hypothesis based on in silico, in vitro and in vivo evidences. Understanding the impact of β-lactam antibiotics on the immune cell will offer new therapeutic clues and new clinical approaches in oncology, immunology, and infectious diseases.
Local Immune Control of Latent Herpes Simplex Virus Type 1 in Ganglia of Mice and Man
Authors: St. Leger Anthony J. , Koelle David M. , Kinchington Paul R. , Verjans Georges Michel G. M.
Published: September 2021

Herpes simplex virus type 1 (HSV-1) is a prevalent human pathogen. HSV-1 genomes persist in trigeminal ganglia neuronal nuclei as chromatinized episomes, while epithelial cells are typically killed by lytic infection. Fluctuations in anti-viral responses, broadly defined, may underlay periodic reactivations. The ganglionic immune response to HSV-1 infection includes cell-intrinsic responses in neurons, innate sensing by several cell types, and the infiltration and persistence of antigen-specific T-cells. The mechanisms specifying the contrasting fates of HSV-1 in neurons and epithelial cells may include differential genome silencing and chromatinization, dictated by variation in access of immune modulating viral tegument proteins to the cell body, and protection of neurons by autophagy. Innate responses have the capacity of recruiting additional immune cells and paracrine activity on parenchymal cells, for exampleviachemokines and type I interferons. In both mice and humans, HSV-1-specific CD8 and CD4 T-cells are recruited to ganglia, with mechanistic studies suggesting active roles in immune surveillance and control of reactivation. In this review we focus mainly on HSV-1 and the TG, comparing and contrasting where possible observational, interventional, andin vitrostudies between humans and animal hosts.
Contribution to HIV Prevention and Treatment by Antibody-Mediated Effector Function and Advances in Broadly Neutralizing Antibody Delivery by Vectored Immunoprophylaxis
Authors: Phelps Meredith , Balazs Alejandro Benjamin
Published: September 2021

HIV-1 broadly neutralizing antibodies (bNAbs) targeting the viral envelope have shown significant promise in both HIV prevention and viral clearance, including pivotal results against sensitive strains in the recent Antibody Mediated Prevention (AMP) trial. Studies of bNAb passive transfer in infected patients have demonstrated transient reduction of viral load at high concentrations that rebounds as bNAb is cleared from circulation. While neutralization is a crucial component of therapeutic efficacy, numerous studies have demonstrated that bNAbs can also mediate effector functions, such as antibody-dependent cellular cytotoxicity (ADCC), antibody-dependent cellular phagocytosis (ADCP), and antibody-dependent complement deposition (ADCD). These functions have been shown to contribute towards protection in several models of HIV acquisition and in viral clearance during chronic infection, however the role of target epitope in facilitating these functions, as well as the contribution of individual innate functions in protection and viral clearance remain areas of active investigation. Despite their potential, the transient nature of antibody passive transfer limits the widespread use of bNAbs. To overcome this, we and others have demonstrated vectored antibody delivery capable of yielding long-lasting expression of bNAbs in vivo. Two clinical trials have shown that adeno-associated virus (AAV) delivery of bNAbs is safe and capable of sustained bNAb expression for over 18 months following a single intramuscular administration. Here, we review key concepts of effector functions mediated by bNAbs against HIV infection and the potential for vectored immunoprophylaxis as a means of producing bNAbs in patients.
Sub-Optimality of the Early Visual System Explained Through Biologically Plausible Plasticity
Authors: Chauhan Tushar , Masquelier Timothée , Cottereau Benoit R.
Published: September 2021

The early visual cortex is the site of crucial pre-processing for more complex, biologically relevant computations that drive perception and, ultimately, behaviour. This pre-processing is often studied under the assumption that neural populations are optimised for the most efficient (in terms of energy, information, spikes, etc.) representation of natural statistics. Normative models such as Independent Component Analysis (ICA) and Sparse Coding (SC) consider the phenomenon as a generative, minimisation problem which they assume the early cortical populations have evolved to solve. However, measurements in monkey and cat suggest that receptive fields (RFs) in the primary visual cortex are often noisy, blobby, and symmetrical, making them sub-optimal for operations such as edge-detection. We propose that this suboptimality occurs because the RFs do not emerge through a global minimisation of generative error, but through locally operating biological mechanisms such as spike-timing dependent plasticity (STDP). Using a network endowed with an abstract, rank-based STDP rule, we show that the shape and orientation tuning of the converged units are remarkably close to single-cell measurements in the macaque primary visual cortex. We quantify this similarity using physiological parameters (frequency-normalised spread vectors), information theoretic measures [Kullback–Leibler (KL) divergence and Gini index], as well as simulations of a typical electrophysiology experiment designed to estimate orientation tuning curves. Taken together, our results suggest that compared to purely generative schemes, process-based biophysical models may offer a better description of the suboptimality observed in the early visual cortex.
Gimap5 Inhibits Lung Cancer Growth by Interacting With M6PR
Authors: Dai Pei , Tang Zhongxiang , Ruan Pinglang , Bajinka Ousman , Liu Dan , Tan Yurong
Published: September 2021

Objective

Several studies have demonstrated the impacts of GTPases of immunity-associated proteins (GIMAPs) on malignant cells. However, the mechanisms through which Gimap5 regulates lung cancer cells are yet to be thoroughly investigated in the literature. Our study aimed to investigate the function of Gimap5 in the development of lung cancer.

Methods

The expression levels of the GIMAP family were analyzed in lung cancer patients of various cancer databases and lung cancer cell lines. After the survival rates of the cells were analyzed, we constructed Gimap5 over-expressed lung cancer cell lines and assessed the effects of Gimap5 on cell migration, cell invasion, cell proliferation and the epithelial-mesenchymal transition (EMT). We later screened the interacting proteins of Gimap5 using Co-IP combined with mass spectrometry and then analyzed the expression and distribution of M6PR, including its impacts on protein-arginine deiminase type-4 (PADI4).

Results

Findings indicated that GIMAP family expression decreased significantly in lung cancer cell lines. We also noticed that the downregulation of the GIMAP family was related to the poor prognosis of lung cancer patients. Our experimental results showed that Gimap5 could inhibit the migration, invasion, proliferation and EMT of lung cancer cell lines. Moreover, we found that Gimap5 promoted the transport of M6PR from the cytoplasm to the cell membrane, thereby inhibiting the enhancement of EMT-related PADI4.

Conclusion

Our research suggested that Gimap5 could inhibit the growth of lung cancer by interacting with M6PR and that it could be a potential biomarker for the diagnosis and prognosis of lung cancer.
Clinical Outcome of CT-Guided Stereotactic Ablative Brachytherapy for Unresectable Early Non-Small Cell Lung Cancer: A Retrospective, Multicenter Study
Authors: Ji Zhe , Huo Bin , Liu Shifeng , Liang Qinghua , Xing Chao , Hu Miaomiao , Ma Yanli , Wang Zhe , Zhao Xinxin , Song Yuqing , Wang Yufeng , Han Hongmei , Zhang Kaixian , Wang Ruoyu , Chai Shude , Huang Xuequan , Hu Xiaokun , Wang Junjie
Published: September 2021

Objective

To analyze the efficacy and safety of low dose rate stereotactic ablative brachytherapy (L-SABT) for treatment of unresectable early-stage non-small cell lung cancer (NSCLC).

Methods

Data of patients with early-stage NSCLC who received CT-guided L-SABT (radioactive I-125 seeds implantation) at eight different centers from December 2010 to August 2020 were retrospectively analyzed. Treatment efficacy and complications were evaluated.

Results

A total of 99 patients were included in this study. Median follow-up duration was 46.3 months (6.1-119.3 months). The 1-year, 3-year, and 5-year local control rates were 89.1%, 77.5%, and 75.7%, respectively. The 1-year, 3-year, and 5-year overall survival rates were 96.7%, 70.1%, and 54.4%, respectively. Treatment failure occurred in 38.4% of patients. Local/regional recurrence, distant metastasis, and recurrence combined with metastasis accounted for 15.1%, 12.1%, and 11.1%, respectively. Pneumothorax occurred in 47 patients (47.5%) with 19 cases (19.2%) needing closed drainage. The only radiation-related adverse reaction was two cases of grade 2 radiation pneumonia. KPS 80–100, T1, the lesion was located in the left lobe, GTV D90 ≥150 Gy and the distance between the lesion and chest wall was < 1 cm, were associated with better local control (all P < 0.05); on multivariate analysis KPS, GTV D90, and the distance between the lesion and chest wall were independent prognostic factors for local control (all P < 0.05). KPS 80–100, T1, GTV D90 ≥150 Gy, and the distance between the lesion and chest wall was < 1 cm were also associated with better survival (all P < 0.05); on multivariate analysis KPS, T stage, and GTV D90 were independent prognostic factors for survival (all P < 0.05). The incidence of pneumothorax in patients with lesions <1 cm and ≥1cm from the chest wall was 33.3% and 56.7%, respectively, and the differences were statistically significant (P = 0.026).

Conclusion

L-SABT showed acceptable efficacy in the treatment of unresectable early-stage NSCLC. But the incidence of pneumothorax is high. For patients with T1 stage and lesions <1 cm from the chest wall, it may have better efficacy. Prescription dose greater than 150 Gy may bring better results.
Trimetazidine Attenuates Heart Failure by Improving Myocardial Metabolism via AMPK
Authors: Shu Hongyang , Hang Weijian , Peng Yizhong , Nie Jiali , Wu Lujin , Zhang Wenjun , Wang Dao Wen , Zhou Ning
Published: September 2021

Energic deficiency of cardiomyocytes is a dominant cause of heart failure. An antianginal agent, trimetazidine improves the myocardial energetic supply. We presumed that trimetazidine protects the cardiomyocytes from the pressure overload-induced heart failure through improving the myocardial metabolism. C57BL/6 mice were subjected to transverse aortic constriction (TAC). After 4 weeks of TAC, heart failure was observed in mice manifested by an increased left ventricular (LV) chamber dimension, an impaired LV ejection fraction evaluated by echocardiography analysis, which were significantly restrained by the treatment of trimetazidine. Trimetazidine restored the mitochondrial morphology and function tested by cardiac transmission electron microscope and mitochondrial dynamic proteins analysis. Positron emission tomography showed that trimetazidine significantly elevated the glucose uptake in TAC mouse heart. Trimetazidine restrained the impairments of the insulin signaling in TAC mice and promoted the translocation of glucose transporter type IV (GLUT4) from the storage vesicle to membrane. However, these cardioprotective effects of trimetazidine in TAC mice were notably abolished by compound C (C.C), a specific AMPK inhibitor. The enlargement of neonatal rat cardiomyocyte induced by mechanical stretch, together with the increased expression of hypertrophy-associated proteins, mitochondria deformation and dysfunction were significantly ameliorated by trimetazidine. Trimetazidine enhanced the isolated cardiomyocyte glucose uptake in vitro. These benefits brought by trimetazidine were also removed with the presence of C.C. In conclusion, trimetazidine attenuated pressure overload-induced heart failure through improving myocardial mitochondrial function and glucose uptake via AMPK.
Bisbenzylisoquinoline Alkaloids of Cissampelos Sympodialis With in Vitro Antiviral Activity Against Zika Virus
Authors: da Silva Poliana Gomes , Fonseca Aventino H. , Ribeiro Malu P. , Silva Taizia D. , Grael Cristiane F. F. , Pena Lindomar J. , Silva Tania M. S. , Oliveira Eduardo de Jesus
Published: September 2021

In search of new antiviral compounds against Zika virus we conducted a bioassay-guided fractionation of bisbenzyilisoquinoline alkaloids isolated from Cissampelos sympodialis (Menispermaceae), a medicinal plant species endemic to Brazil. Six subfractions were obtained from a tertiary alkaloidal fraction of the rhizomes (TAFrz) using preparative high-performance liquid chromatography. All the subfractions were tested against Zika virus-infected Vero cells as the cellular model to evaluate cytotoxicity and antiviral effective concentrations. The results showed that three of the six TAFrz subfractions tested were active. The most active ones were the subfraction 6 (that consisted of the alkaloids methylwarifteine and warifteine present as a mixture at a ratio of 8.8:1.2 respectively) and the subfraction 5, that was later identified as warifteine, the major tertiary alkaloid of this species. Warifteine was able to significantly reduce virus titer in Zika virus-infected Vero cells with an IC50 of 2.2 μg/ml and this effect was selective (selectivity index, SI = 68.3). Subfraction 6 had an IC50 = 3.5 μg/ml and was more cytotoxic than pure warifteine, with SI = 6.14. Fraction 5 and fraction 6 were more potent in decreasing the viral titer of Zika virus-infected Vero cells than 6-methylmercaptopurine riboside (IC50 = 24.5 μg/ml and SI = 11.9), a mercaptopurine riboside with ZIKV antiviral activity used as a positive control. Our data demonstrate that alkaloids of the bisbenzylisoquinoline type may be explored as new antiviral agents or as an useful pharmacophore for investigating ZIKV antiviral activity.
Consumer Flow Experience of Senior Citizens in Using Social Media for Online Shopping
Authors: Xu Ying , Wang Yixuan , Khan Asif , Zhao Ranlin
Published: September 2021

The senior market signifies an enormous, rapidly expanding segment, and this research aimed to investigate this segment by proposing a theoretical model incorporating the antecedents of consumer flow experience, flow theory, and technology acceptance model (TAM) devised for determining social media purchase intention. This study focuses on the senior citizens engaged in shopping using social media located in Pakistan. A total of 300 senior citizens were selected. An online survey was conducted with the help of a marketing research agency located in Pakistan. The data were analyzed using the partial least squares (PLS) method. According to the results, the antecedents, such as feedback, enjoyment, and time distortion were found to be in a positive relationship with flow experience, however, the concentration did not have a significant effect on the flow. Furthermore, the flow was found to be in a significant relationship with social media purchase intention and TAM. Finally, TAM was also found to be in a positive significant relationship with social media purchase intention. This research contributes to the constantly expanding volume of the utilization of social media by the senior market segment population for buying and producing the highly valuable knowledge for manufacturers, wholesalers, vendors, and a huge number of senior customers.
The Degree of Linear Polarization for Suspended Particle Fields from Diverse Natural Waters
Authors: Zhai Siyao , Twardowski Michael
Published: September 2021

The Degree of Linear Polarization (DoLP) for unperturbed particle fields in waters from six diverse regions around the globe was measured with the custom Multi-Angle Scattering Optical Tool (MASCOT). DoLP here is defined as the ratio of two elements of Mueller scattering matrix, i.e., -M12/M11. Field sites covered inland waters, coastal oceans and open oceans, including both ocean color Case I and II water types. The angular shape of the measured particulate DoLP was analyzed in detail for each field site and for the ensemble average. Three parameters used to quantitatively characterize DoLP shape were the symmetry with respect to 90°, peak magnitude, and peak angle of measured DoLP angular curve. Vertical profiles of particulate DoLP were analyzed with maximum recorded depth of 111 m. Converse to Rayleigh scatterers, we found measured particulate DoLPs were not symmetric with respect to 90°. On average, DoLP peaks were shifted slightly toward larger angles, with most falling between estimated values of 90° and 95°. All particulate DoLP peak magnitudes generally varied within [0.6, 0.9]. Lorenz-Mie (homogeneous sphere) light scattering theory was used to construct a new inversion for bulk particulate refractive index from a lookup table based on DoLP and spectral attenuation measurements. We compared the Mie-DoLP-based particulate refractive index retrieval with the backscattering-based model from (Twardowski et al., J. Geophys. Res., 2001, 106(C7), 14,129–14,142). Particulate refractive index retrieved with the two models were in some cases comparable. At two of the six field sites we saw good agreement between the two models, whereas at another two field sites we observed large discrepancies between the two models. Further investigation on the choice of the modeled particle shapes and compositions may improve this retrieval approach. Results are compatible with previous studies on DoLPs in natural waters and comprehensive observations are provided on the particulate DoLP angular shape, vertical profile and global distributions that are important for future vector radiative transfer simulations. This study is relevant to future PACE polarimeters and associated remote retrieval of oceanic particle composition using polarimetry.
Black Sea Jellyfish: Shocking Newcomers to Suisun Marsh
Authors: Newell Caroline L. , Durand John R. , Meek Mariah H. , Moyle Peter B.
Published: September 2021

Black Sea jellyfish love to travel. Far from their original home in the Black Sea of Southeastern Europe, Black Sea jellyfish have come to the San Francisco Estuary. Since these jellyfish first started regularly appearing in fishing nets in the early 1980s, scientists have been finding more and more of them in the San Francisco Estuary. Over the past 40 years, our lab has tracked the numbers of Black Sea jellyfish in a part of the Estuary called the Suisun Marsh. Over this time, we have observed increasing amounts of jellies, with the highest amount ever recorded caught in 2019. What does this mean for the San Francisco Estuary and its other current residents? This is a question we will explore in this article.
Internal Locus of Control and Sense of Coherence Decrease During the COVID-19 Pandemic: A Survey of Students and Professionals in Social Work
Authors: Misamer Melanie , Signerski-Krieger Jörg , Bartels Claudia , Belz Michael
Published: September 2021

Mental health is severely challenged by the COVID-19 pandemic due to a variety of restrictions in public and private life. Students in particular may face additional and unique stressors: face-to-face classes have been largely replaced by digital formats, leading to further reduced social contacts, thus facilitating the development of psychological symptoms. In this study, we aimed to assess the impact of the current peri-pandemic situation on students’ 1) locus of control and 2) sense of coherence, both of which have been linked to mental health in previous work. A total of 403 social work students from Germany participated, providing both retrospective (pre-pandemic) and current (February/March 2021) ratings. Furthermore, 324 social work professionals were included to analyze differences between both groups. Locus of control shifted significantly from internal to external during the pandemic for students and professionals. Furthermore, high mental burden correlated with increased external and decreased internal locus of control. Sense of coherence decreased during the pandemic for the entire sample and correlated with high mental burden. Overall, students showed a stronger drop compared to professionals, primarily due to a more pronounced decrease in perceived meaningfulness (all p < 0.001). In summary, students and professionals responded with increased feelings of powerlessness in the absence of sufficient coping strategies—this could lead to further deterioration of mental health as the pandemic continues. In this context, students appear to be particularly vulnerable to a reduction in sense of coherence. We conclude that interventions to improve coping strategies are urgently needed.
Proof of Principle of a Fuel Injector Based on a Magnetostrictive Actuator
Authors: Allocca Luigi , Davino Daniele , Montanaro Alessandro , Visone Ciro
Published: September 2021

One of the goals of modern internal combustion engines is the NOx-soot trade-off, and this would be better achieved by a better control of the fuel injection. Moreover, this feature can be also useful for high-performance hydraulic systems. Actual fuel injection technology either allows only the control of the injection time or it is based on very complex mechanical-hydraulic systems, as in the case of piezo-actuators. This work describes the basic steps that brought the authors to the realization of a concept fuel injector based on a Terfenol-D magnetostrictive actuator that could overcome the previous issues, being both simple and controllable. The study provides the design, development, and a feasibility analysis of a magnetostrictive actuator for fuel injection, by providing a basic magneto-static analysis of the actuator, the adaptation of a suitable standard fuel injector, and its experimental testing in a lab environment, with different shapes and amplitude of the reference signal to follow.
The Effect of Spool Displacement Control to the Flow Rate in the Piezoelectric Stack-Based Valve System Subjected to High Operating Temperature
Authors: Park Yu-Jin , Kim Bo-Gyu , Jeon Jun-Cheol , Jung Dongsoo , Choi Seung-Bok
Published: September 2021

This work investigates the effect of spool displacement control of the piezoelectric stack actuator (PSA) based valve system on the flow motion of the pressure drop and flow rate. As a first step, the governing equations of the structural parts of the displacement amplifier and spool are derived, followed by the governing equation of the fluid part considering control volume and steady flow force. Then, an appropriate size of the valve is designed and manufactured. An experimental apparatus to control the spool displacement is set up in the heat chamber and tracking control for the spool displacement is evaluated at 20 °C and 100 °C by implementing a proportional-integral-derivative (PID) feedback controller. The tracking controls of the spool displacement associated with the sinusoidal and triangular trajectories are realized at 20 °C and 100 °C. It is demonstrated that the tracking controls for the sinusoidal and triangular trajectories have been well carried out showing the tracking error less than 3 μm at both temperatures. In addition, the flow motions for the pressure drop and the flow rate of the proposed valve system are experimentally investigated. It is identified from this investigation that both pressure drop and flow rate evaluated 20 °C have been decreased up to 18% at 100 °C. This result directly indicates that the temperature effect to control performance of the structural part and fluid part in the proposed PSA based valve system is different and hence careful attention is required to achieve the successful development of advanced valve systems subjected to a wide range of the operating temperature.