bluuebunny/crossref_metadata_2025 huggingface dataset:
A Review on Flexible Electrochemical Biosensors to Monitor Alcohol in Sweat
Authors: Costa Nuna G. , Antunes Joana C. , Paleo Antonio J. , Rocha Ana M.
Published: April 2022

The continued focus on improving the quality of human life has encouraged the development of increasingly efficient, durable, and cost-effective products in healthcare. Over the last decade, there has been substantial development in the field of technical and interactive textiles that combine expertise in electronics, biology, chemistry, and physics. Most recently, the creation of textile biosensors capable of quantifying biometric data in biological fluids is being studied, to detect a specific disease or the physical condition of an individual. The ultimate goal is to provide access to medical diagnosis anytime and anywhere. Presently, alcohol is considered the most commonly used addictive substance worldwide, being one of the main causes of death in road accidents. Thus, it is important to think of solutions capable of minimizing this public health problem. Alcohol biosensors constitute an excellent tool to aid at improving road safety. Hence, this review explores concepts about alcohol biomarkers, the composition of human sweat and the correlation between alcohol and blood. Different components and requirements of a biosensor are reviewed, along with the electrochemical techniques to evaluate its performance, in addition to construction techniques of textile-based biosensors. Special attention is given to the determination of biomarkers that must be low cost and fast, so the use of biomimetic materials to recognize and detect the target analyte is turning into an attractive option to improve electrochemical behavior.
Experimental Study on Operating Characteristic of a Combined Radiant Floor and Fan Coil Cooling System in a High Humidity Environment
Authors: Zhu Xuwei , Liu Jiying , Zhu Xiangyuan , Wang Xiaole , Du Yanqiu , Miao Jikui
Published: April 2022

The combined radiant floor and fan coil cooling (RFCAFC) system is widely used due to its high comfort and large energy saving potential. In this study, as an example, the combined RFCAFC system was studied in a high humidity environment in Jinan, Shandong Province, China. The novelty of the combined RFCAFC system lies in its ability to automatically adjust the water supply temperature of the radiant floor and fan coil in real time according to outdoor meteorological conditions, achieving thermal comfort while eliminating the likelihood of condensation on the radiant floor surface. Days with similar outdoor meteorological conditions were grouped, and the comfort level and hourly cooling performance coefficient (EERh) of different operating strategies for different outdoor meteorological conditions were monitored along with other evaluation indicators. The RFCAFC had good energy efficiency and comfort in a high humidity room environment. This study showed that the indoor vertical air temperature difference ranged from 1.6 to 1.8 °C, which met the ASHRAE55-2017 standard. The radiant floor surface temperature uniformity coefficient (S) fluctuated between 0.7 and 1.0, and the time it took the radiant floor surface temperature to reach 63.2% of the total variability range (τ63) for different operation strategies based on different outdoor meteorological conditions ranged between 4.4 and 4.7 h, which was within the normal range. The proportion of the total cooling capacity contributed by fan coil cooling under low temperature and high humidity (LH), high temperature and low humidity (HL), and medium temperature and medium humidity (MM) were 68.0%, 73.8%, and 71.7%, respectively. Based on this study, the following recommendations for the combined cooling system can be made: (1) When the outdoor humidity is high, the radiant floor system should be turned on early to provide cooling capacity. When the outdoor temperature is high, the fan coil system should be turned on early to reduce the indoor temperature. (2) To reduce energy consumption and achieve efficient operation of the system, the radiant floor system should be continuously operated to maximize its contribution to the cooling capacity, while the fan coil can be operated intermittently. Natural cooling can be integrated to provide additional cooling capacity to the room in the hours preceding occupation (i.e., 7:00–9:00). (3) The operation strategy of the combined cooling system must be able to respond in real time to changes in outdoor meteorological conditions to prevent discomfort in times of extreme heat or humidity.
Mechanistic Insights into the Link between Gut Dysbiosis and Major Depression: An Extensive Review
Authors: Sonali Sharma , Ray Bipul , Ahmed Tousif Hediyal , Rathipriya Annan Gopinath , Sunanda Tuladhar , Mahalakshmi Arehally M. , Rungratanawanich Wiramon , Essa Musthafa Mohamed , Qoronfleh M. Walid , Chidambaram Saravana Babu , Song Byoung-Joon
Published: April 2022

Depression is a highly common mental disorder, which is often multifactorial with sex, genetic, environmental, and/or psychological causes. Recent advancements in biomedical research have demonstrated a clear correlation between gut dysbiosis (GD) or gut microbial dysbiosis and the development of anxiety or depressive behaviors. The gut microbiome communicates with the brain through the neural, immune, and metabolic pathways, either directly (via vagal nerves) or indirectly (via gut- and microbial-derived metabolites as well as gut hormones and endocrine peptides, including peptide YY, pancreatic polypeptide, neuropeptide Y, cholecystokinin, corticotropin-releasing factor, glucagon-like peptide, oxytocin, and ghrelin). Maintaining healthy gut microbiota (GM) is now being recognized as important for brain health through the use of probiotics, prebiotics, synbiotics, fecal microbial transplantation (FMT), etc. A few approaches exert antidepressant effects via restoring GM and hypothalamus–pituitary–adrenal (HPA) axis functions. In this review, we have summarized the etiopathogenic link between gut dysbiosis and depression with preclinical and clinical evidence. In addition, we have collated information on the recent therapies and supplements, such as probiotics, prebiotics, short-chain fatty acids, and vitamin B12, omega-3 fatty acids, etc., which target the gut–brain axis (GBA) for the effective management of depressive behavior and anxiety.
Cysteine-Rich LIM-Only Protein 4 (CRP4) Promotes Atherogenesis in the ApoE−/− Mouse Model
Authors: Längst Natalie , Adler Julia , Kuret Anna , Peter Andreas , Ruth Peter , Boldt Karsten , Lukowski Robert
Published: April 2022

Vascular smooth muscle cells (VSMCs) can switch from their contractile state to a synthetic phenotype resulting in high migratory and proliferative capacity and driving atherosclerotic lesion formation. The cysteine-rich LIM-only protein 4 (CRP4) reportedly modulates VSM-like transcriptional signatures, which are perturbed in VSMCs undergoing phenotypic switching. Thus, we hypothesized that CRP4 contributes to adverse VSMC behaviours and thereby to atherogenesis in vivo. The atherogenic properties of CRP4 were investigated in plaque-prone apolipoprotein E (ApoE) and CRP4 double-knockout (dKO) as well as ApoE-deficient CRP4 wildtype mice. dKO mice exhibited lower plaque numbers and lesion areas as well as a reduced content of α-smooth muscle actin positive cells in the lesion area, while lesion-associated cell proliferation was elevated in vessels lacking CRP4. Reduced plaque volumes in dKO correlated with significantly less intra-plaque oxidized low-density lipoprotein (oxLDL), presumably due to upregulation of the antioxidant factor peroxiredoxin-4 (PRDX4). This study identifies CRP4 as a novel pro-atherogenic factor that facilitates plaque oxLDL deposition and identifies the invasion of atherosclerotic lesions by VSMCs as important determinants of plaque vulnerability. Thus, targeting of VSMC CRP4 should be considered in plaque-stabilizing pharmacological strategies.
Tet1 Suppresses p21 to Ensure Proper Cell Cycle Progression in Embryonic Stem Cells
Authors: Chrysanthou Stephanie , Flores Julio C. , Dawlaty Meelad M.
Published: April 2022

Ten eleven translocation 1 (Tet1) is a DNA dioxygenase that promotes DNA demethylation by oxidizing 5-methylcytosine. It can also partner with chromatin-activating and repressive complexes to regulate gene expressions independent of its enzymatic activity. Tet1 is highly expressed in embryonic stem cells (ESCs) and regulates pluripotency and differentiation. However, its roles in ESC cell cycle progression and proliferation have not been investigated. Using a series of Tet1 catalytic mutant (Tet1m/m), knockout (Tet1−/−) and wild type (Tet1+/+) mouse ESCs (mESCs), we identified a non-catalytic role of Tet1 in the proper cell cycle progression and proliferation of mESCs. Tet1−/−, but not Tet1m/m, mESCs exhibited a significant reduction in proliferation and delayed progression through G1. We found that the cyclin-dependent kinase inhibitor p21/Cdkn1a was uniquely upregulated in Tet1−/− mESCs and its knockdown corrected the slow proliferation and delayed G1 progression. Mechanistically, we found that p21 was a direct target of Tet1. Tet1 occupancy at the p21 promoter overlapped with the repressive histone mark H3K27me3 as well as with the H3K27 trimethyl transferase PRC2 component Ezh2. A loss of Tet1, but not loss of its catalytic activity, significantly reduced the enrichment of Ezh2 and H3K27 trimethylation at the p21 promoter without affecting the DNA methylation levels. We also found that the proliferation defects of Tet1−/− mESCs were independent of their differentiation defects. Together, these findings established a non-catalytic role for Tet1 in suppressing p21 in mESCs to ensure a rapid G1-to-S progression, which is a key hallmark of ESC proliferation. It also established Tet1 as an epigenetic regulator of ESC proliferation in addition to its previously defined roles in ESC pluripotency and differentiation.
Electrodeposition of Calcium Phosphate Coatings on Metallic Substrates for Bone Implant Applications: A Review
Authors: Drevet Richard , Benhayoune Hicham
Published: April 2022

This review summaries more than three decades of scientific knowledge on electrodeposition of calcium phosphate coatings. This low-temperature process aims to make the surface of metallic bone implants bioactive within a physiological environment. The first part of the review describes the reaction mechanisms that lead to the synthesis of a bioactive coating. Electrodeposition occurs in three consecutive steps that involve electrochemical reactions, pH modification, and precipitation of the calcium phosphate coating. However, the process also produces undesired dihydrogen bubbles during the deposition because of the reduction of water, the solvent of the electrolyte solution. To prevent the production of large amounts of dihydrogen bubbles, the current density value is limited during deposition. To circumvent this issue, the use of pulsed current has been proposed in recent years to replace the traditional direct current. Thanks to breaking times, dihydrogen bubbles can regularly escape from the surface of the implant, and the deposition of the calcium phosphate coating is less disturbed by the accumulation of bubbles. In addition, the pulsed current has a positive impact on the chemical composition, morphology, roughness, and mechanical properties of the electrodeposited calcium phosphate coating. Finally, the review describes one of the most interesting properties of electrodeposition, i.e., the possibility of adding ionic substituents to the calcium phosphate crystal lattice to improve the biological performance of the bone implant. Several cations and anions are reviewed from the scientific literature with a description of their biological impact on the physiological environment.
The Effect of Annealing on the Optoelectronic Properties and Energy State of Amorphous Pyrochlore Y2Ti2O7 Thin Layers by Sol–Gel Synthesis
Authors: Ting Hsiang-An , Chen Yong-Yu , Li Zong-Ming , Hsieh Ya-Ping , Chiu Sheng-Kuei , Ting Chu-Chi
Published: April 2022

Pyrochlore titanate (Y2Ti2O7) is a promising material for a wide range of applications in optoelectronics and photocatalysis due to its advantageous chemical, mechanical, and optical properties. To enhance its potential for such uses, however, a high-quality and scalable synthesis method is required. We here investigate the crystallization of sol–gel produced Y2Ti2O7 layers. We observe a transition of the amorphous pyrochlore phase at annealing temperatures below 700 °C. The transmittances of the Y2Ti2O7 thin layers annealed at 400 to 700 °C are approximately 92.3%. The refractive indices and packing densities of Y2Ti2O7 thin layers annealed at 400–700 °C/1 h vary from 1.931 to 1.954 and 0.835 to 0.846, respectively. The optical bandgap energies of Y2Ti2O7 thin layers annealed at 400–700 °C/1 h reduce from 4.356 to 4.319 eV because of the Moss–Burstein effect. These good electronic and optical properties make Y2Ti2O7 thin layers a promising host material for many potential applications.
DTI Abnormalities Related to Glioblastoma: A Prospective Comparative Study with Metastasis and Healthy Subjects
Authors: El Ouadih Youssef , Pereira Bruno , Biau Julian , Claise Béatrice , Chaix Rémi , Verrelle Pierre , Khalil Toufik , Durando Xavier , Lemaire Jean-Jacques
Published: April 2022

(1) Background: Glioblastoma multiforme (GBM) shows complex mechanisms of spreading of the tumor cells, up to remote areas, and little is still known of these mechanisms, thus we focused on MRI abnormalities observable in the tumor and the brain adjacent to the lesion, up to the contralateral hemisphere, with a special interest on tensor diffusion imaging informing on white matter architecture; (2) Material and Methods: volumes, macroscopic volume (MV), brain-adjacent-tumor (BAT) volume and abnormal color-coded DTI volume (aCCV), and region-of-interest samples (probe volumes, ipsi, and contra lateral to the lesion), with their MRI characteristics, apparent diffusion coefficient (ADC), fractional anisotropy (FA) values, and number of fibers (DTI fiber tracking) were analyzed in patients suffering GBM (n = 15) and metastasis (n = 9), and healthy subjects (n = 15), using ad hoc statistical methods (type I error = 5%) (3) Results: GBM volumes were larger than metastasis volumes, aCCV being larger in GBM and BAT ADC was higher in metastasis, ADC decreased centripetally in metastasis, FA increased centripetally either in GBM or metastasis, MV and BAT FA values were higher in GBM, ipsi FA values of GBM ROIs were higher than those of metastasis, and the GBM ipsi number of fibers was higher than the GBM contra number of fibers; (4) Conclusions: The MV, BAT and especially the aCCV, as well as their related water diffusion characteristics, could be useful biomarkers in oncology and functional oncology.
Motivation to Consent and Adhere to the FORT Randomized Controlled Trial
Authors: Maheu Christine , Lok Valerie , Galica Jacqueline , Tse Mali , Maltus Emma , Giguère Lauriane , Tock Wing Lam , Lebel Sophie
Published: April 2022

The aim of this qualitative study was to identify the motivational factors that influence cancer survivors to participate and adhere to the fear of cancer recurrence (FCR) FORT randomized controlled trial (RCT). Fifteen women diagnosed with breast and gynecological cancer who took part in the FORT RCT were interviewed about their experience to consent and adhere to the trial. The transcribed interviews were content analyzed within a relational autonomy framework. The analysis revealed that the participants’ motivation to consent and adhere to the FORT RCT was structured around thirteen subthemes grouped into four overarching themes: (1) Personal Influential Factors; (2) Societal Motivations; (3) Structural Influences; and (4) Gains in Emotional Support. The unique structures of the trial such as the group format, the friendships formed with other participants in their group and with the group leaders, and the right timing of the trial within their cancer survivorship trajectory all contributed to their motivation to consent and adhere to the FORT RCT. While their initial motivation to participate was mostly altruistic, it was their personal gains obtained over the course of the trial that contributed to their adherence. Potential gains in emotional and social support from psycho-oncology trials should be capitalized when approaching future participants as a mean to improve on motivations to consent and adhere.
Utility of Red Cell Distribution Width (RDW) as a Noninvasive Biomarker for the Diagnosis of Acute Appendicitis: A Systematic Review and Meta-Analysis of 5222 Cases
Authors: Anand Sachit , Krishnan Nellai , Jukić Miro , Križanac Zvonimir , Llorente Muñoz Carlos Martin , Pogorelić Zenon
Published: April 2022

Background: Despite great advances in medicine, numerous available laboratory markers, and radiological imaging, the diagnosis of acute appendicitis (AA) in some cases still remains controversial and challenging for clinicians. Because of that, clinicians are still looking for an ideal marker that would be specific to AA. The red blood cell distribution width (RDW) has been recently investigated in several studies as a potential biomarker for AA. The aim of this systematic review and meta-analysis was to systematically summarize and compare all relevant data on RDW as a diagnostic biomarker for AA. Methods: This systematic review and meta-analysis were performed as per the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. Scientific databases (PubMed, Scopus, Web of Science, and Excerpta Medica database—EMBASE) were systematically searched for relevant comparative studies by two independent researches using keywords ((red cell distribution width) OR rdw) AND (appendicitis). An independent assessment of the methodological quality was performed by two authors using the Downs and Black scale. RevMan 5.4 software was used to perform the meta-analysis. Results: Fifteen studies were included in the final meta-analysis; the majority of the studies was retrospective. Nine studies compared the RDW values between AA and non-AA; four studies compared the same between AA and healthy controls, while two studies compared the RDW values among all three groups. The estimated heterogeneity among the studies for all outcome was statistically significant (I2 = 92–99%, p < 0.00001). The pooling the data demonstrated no statistically significant difference in the RDW values (weighted mean difference (WMD) = 0.03, 95% CI = (−0.46, 0.52), p = 0.91) between AA and healthy controls as well as between AA and non-AA cases (WMD = 0.23, 95%CI = (–0.19, 0.65), p = 0.28). A separate subanalysis was performed to evaluate the utility of this biomarker for the pediatric age group. Pooling the data demonstrated no significant difference among the AA and non-AA groups in terms of the RDW values (WMD = 0.99, 95% CI = (–0.35, 2.33), p = 0.15). Conclusion: The RDW value difference demonstrated no statistically significant difference in AA versus healthy individuals and AA versus non-AA individuals. At the moment, there is no evidence of RDW utility in diagnostic testing of AA. Further research with prospective, multicenter studies and studies targeting special patient groups with a large sample size are needed in this field.
Splenic Artery Pseudoaneurysms: The Role of ce-CT for Diagnosis and Treatment Planning
Authors: Corvino Fabio , Giurazza Francesco , Ierardi Anna Maria , Lucatelli Pierleone , Basile Antonello , Corvino Antonio , Niola Raffaella
Published: April 2022

Splenic artery pseudoaneurysm (PSA) is a contained vascular wall lesion associated with a high mortality rate, generally related to pancreatitis, trauma, malignancy, iatrogenic injury, and segmental arterial mediolysis. Computed tomography angiography allows us to visualize the vascular anatomy, differentiate a PSA from an aneurysm, and provide adequate information for endovascular/surgical treatment. The present review reports on the main state-of-the-art splenic artery PSA diagnosis, differentiating between the pros and cons of the imaging methods and about the endovascular treatment.
Assessing the Effects of the COVID-19 Pandemic on M-Commerce Adoption: An Adapted UTAUT2 Approach
Authors: Vinerean Simona , Budac Camelia , Baltador Lia Alexandra , Dabija Dan-Cristian
Published: April 2022

The COVID-19 pandemic has impacted consumers’ lives and their shopping patterns, leading them towards mobile commerce. To address current shopping circumstances, an adapted UTAUT2 model aims to integrate trust and perceptions of the effects of the COVID-19 pandemic on consumers’ behavioral intention to rely on m-commerce during this period. The study proposes a research model which is investigated with the help of structural equation modelling in AMOS. Using the framework of a cross-sectional study, data were collected from an emerging market in Romania, where internet speed ranks 4th worldwide and where the yearly increase in internet and social media users is approximately 10%. By using confirmatory factor analysis and structural equation modelling, the research validates the applicability of UTAUT2 in examining consumers’ behavioral intent of using m-commerce during a pandemic. Hedonic motivation is the strongest predictor of consumers’ behavioral intentions to continue using m-commerce. The findings contribute to understandings of consumers’ behavior with m-commerce in an emerging market, extending knowledge based on the adapted UTAUT2 model and allowing for comprehension of the key role of trust and social influences in affecting consumers’ perceptions of the COVID-19 outbreak in relation to shopping patterns.
Simulation about the Effect of the Height-to-Stroke Ratios of Ports on Power and Emissions in an OP2S Engine Using Diesel/Methanol Blends
Authors: Yang Wei , Zhang Lei , Ma Fukang , Xu Dan , Ji Wenjing , Zhao Yangyang , Zhang Jianing
Published: April 2022

Zero carbon emissions will dominate the future of internal combustion engines (ICEs). Existing technology has pushed the performance of ICEs operating on traditional working principles to almost reach their limit. The new generation of ICEs needs to explore new efficient combustion modes. For new combustion modes to simplify the emission after treatment, the opposed-piston, two-stroke (OP2S) diesel engine is a powertrain with great potential value. Combined with dual-fuel technology, the OP2S diesel engine can effectively reduce carbon emissions to achieve clean combustion. Hence, methanol/diesel dual fuel was burnt in the OP2S engine to create a clean combustion mode for future demands. In the present work, a 1D simulation model of an OP2S diesel engine was established and verified. We investigated the influence of port height to stroke ratio (HSR) on power and emission performances of the OP2S diesel engine under different methanol ratios. The results show that the methanol ratio extremely influences the indicated power (IP) with the HSR of intake ports increasing. The IP decreases by about 1.8–2.0% for every 5% increase in methanol. Correspondingly, the methanol ratio extremely influences the indicated thermal efficiency (ITE), with the HSR of exhaust ports increasing. The ITE increases by about 2.1–3.1% for every 5% increase in methanol. The increasing methanol ratio reduces the HSR of ports for the optimal IP and ITE. To balance power performance and emission performance, the methanol ratio should be kept to 10–15%.
Sedimentary Environment and Model for Organic Matter Enrichment: Chang 7 Shale of Late Triassic Yanchang Formation, Southern Margin of Ordos Basin, China
Authors: Zhao Yonggang , Zhang Chunyu , Lu Jungang , Zhu Xingcheng , Li Lei , Si Shanghua
Published: April 2022

Shale oil is an unconventional oil resource that needs to be developed and utilized urgently. However, the Chang 7 shale in the Ordos Basin, as the most typical continental source rock in China, is limited by the study of organic matter (OM) enrichment factors in continental lacustrine facies, and there are still controversies about the controlling factors, which limit the progress of oil and gas exploration. This paper aims to reconstruct the paleoenvironment of Chang 7 shale in the southern margin of Ordos Basin and reveal the controlling factors of organic rich shale by organic and elemental analysis, X-ray diffraction (XRD) analysis, thin section observation, and scanning electron microscopy-energy dispersive spectrometer (SEM-EDS) analysis. The results show that during the deposition period of Chang 7 shale, the climate was warm and humid, the lake water has strong reducing, low salinity and rapid depth changes. Total organic carbon (TOC) is positively correlated with salinity and hydrothermal action and inversely proportional to terrigenous input. The high productivity, low consumption and low dilution result in high enrichment of shale OM in the southern margin of Ordos Basin.
Xenophobic Bullying and COVID-19: An Exploration Using Big Data and Qualitative Analysis
Authors: Dhungana Sainju Karla , Zaidi Huda , Mishra Niti , Kuffour Akosua
Published: April 2022

Extant literature suggests that xenophobic bullying is intensified by isolated national or global events; however, the analysis of such occurrences is methodologically limited to the use of self-reported data. Examining disclosures of racist bullying episodes enables us to contextualize various perspectives that are shared online and generate insights on how COVID-19 has exacerbated the issue. Moreover, understanding the rationale and characteristics present in xenophobic bullying may have important implications for our social wellbeing, mental health, and inclusiveness as a global community both in the short and long term. This study employs a mixed-method approach using Big Data techniques as well as qualitative analysis of xenophobic bullying disclosures on Twitter following the spread of COVID-19. The data suggests that about half of the sample represented xenophobic bullying. The qualitative analysis also found that 64% of xenophobic bullying-related tweets referred to occasions that perpetuated racist stereotypes. Relatedly, the rationale for almost 75% of xenophobic bullying incidents was due to being Chinese or Asian. The findings of this study, coupled with anti-hate reports from around the world, are used to suggest multipronged policy interventions and considerations of how social media sites such as Twitter can be used to curb the spread of misinformation and xenophobic bullying.
Social Support Mediates the Relationship between Body Image Distress and Depressive Symptoms in Prostate Cancer Patients
Authors: Scandurra Cristiano , Muzii Benedetta , La Rocca Roberto , Di Bello Francesco , Bottone Mario , Califano Gianluigi , Longo Nicola , Maldonato Nelson Mauro , Mangiapia Francesco
Published: April 2022

Treatments for prostate cancer (PCa), the second most common cancer in men, may affect the body image (BI) of patients, increasing the risk of negative mental health outcomes. However, an enabling social support network may be a protective factor against the effects of BI distress on health. Therefore, the present study examined the mediating role of social support in the relationship between BI distress and depressive symptoms. Data were retrospectively collected from 197 PCa patients aged from 48 to 79 years (M = 67.19; SD = 6.83). The statistical package for the social sciences with PROCESS Macro was used to assess the direct and mediating effects with bias-corrected bootstrapping (10,000 samples). Results showed that BI distress was positively associated with depressive symptoms and that social support partially mediated this relationship. Moreover, among the different sources of social support, only friend support significantly mediated the association between BI distress and depressive symptoms. This study sheds light on the crucial role of social support as a dimension that can promote health in PCa patients.
Atmospheric Concentration of CO2 and PM2.5 at Salina, Stromboli, and Vulcano Islands (Italy): How Anthropogenic Sources, Ordinary Volcanic Activity and Unrests Affect Air Quality
Authors: Madonia Paolo , Cangemi Marianna , Colajanni Marcello , Winkler Aldo
Published: April 2022

Geogenic and anthropogenic sources of atmospheric particulate and CO2 can lead to threats to human health in volcanic areas. Although the volcanic CO2 hazard is a topic frequently debated in the related scientific literature, space and time distribution of PM2.5 are poorly known. The results of combined CO2/PM2.5 surveys, carried out at Salina, Stromboli, and Vulcano islands (Aeolian archipelago, Italy) in the years 2020–2021, and integrated with investigations on bioaccumulation of metallic particulate matter by the mean of data on the magnetic properties of oleander leaves, are presented in this work. The retrieved results indicate that no significant anthropogenic sources for both CO2 and PM2.5 are active in these islands, at the net of a minor contribution due to vehicular traffic. Conversely, increments in volcanic activity, as the unrest experienced by Vulcano island since the second half of 2021, pose serious threats to human health, due to the near-ground accumulation of CO2, and the presence of suspended micro-droplets of condensed hydrothermal vapor, fostering the diffusion of atmophile viruses, such as the COVID-19. Gas hazard conditions can be generated, not only by volcanic vents or fumarolic fields, but also by unconventional sources, such as the outgassing from shallow hydrothermal aquifers through drilled or hand-carved wells.
Remote Work in a Changing World: A Nod to Personal Space, Self-Regulation and Other Health and Wellness Strategies
Authors: Geldart Sybil
Published: April 2022

Remote work has escalated as a result of the coronavirus pandemic, and citizens have been doing their part to mitigate the spread of viral infection. The downside of quickly switching from a workplace office to remote work is that neither employees nor employers have had time to consciously process the new work environment and formally evaluate health and safety concerns. The aim of this commentary was to make suggestions on how to make remote work more satisfying, safe, and healthy for employees. First, I explored existing research on disease outbreaks and mental stress as the backdrop for discussing health-related strategies. To determine which types of strategies or measures would help, next I examined existing organizational research, including a qualitative study by my colleagues on workers’ perceptions about what makes a healthy workplace. Themes that emerged from the qualitative study align with three broad recommendations discussed in this commentary: cultivating personal space, building in ergonomics, and boosting self-regulation (self-learning) skills. Finally, I suggested that future research should explore the joint roles of the worker and his/her management team in recognition of organizational commitment to occupational health and safety alongside each worker’s need for autonomy in their personal workspace.
Optimization of Shelter Location Based on a Combined Static/Dynamic Two-Stage Optimization Methodology: A Case Study in the Central Urban Area of Xinyi City, China
Authors: Zhong Guangchun , Zhai Guofang , Chen Wei
Published: April 2022

Determining how to reasonably allocate shelters in the central area of the city and improve evacuation efficiency are important issues in the field of urban disaster prevention. This paper introduces the methodology and mathematical model from the field of crowd emergency evacuation to shelter location optimization. Moreover, a shelter location optimization method based on the combination of static network analysis and dynamic evacuation simulation is proposed. The construction costs and evacuation times are taken as the objective functions. In the first stage, based on the static network analysis, a circular evacuation allocation rule based on the gravity model is proposed, and the genetic algorithm is then designed to solve the feasible schemes with the lowest shelter construction costs. In the second stage, the evacuation time is taken as the optimization objective. The age differences of refugees, the selection of evacuation routes, and the behavior of adults helping children and the elderly are simulated in a dynamic evacuation simulation model. The traditional social force model is improved to conduct a regional evacuation simulation and determine the optimal scheme with the shortest evacuation time. Finally, the central urban area of Xinyi City, Jiangsu Province, China, is taken as an empirical case.
Comment on Piña et al. Ten Approaches That Improve Immunostaining: A Review of the Latest Advances for the Optimization of Immunofluorescence. Int. J. Mol. Sci. 2022, 23, 1426
Authors: Sheval Eugene V.
Published: April 2022

With great interest, I have read the article “Ten Approaches That Improve Immunostaining: A Review of the Latest Advances for the Optimization of Immunofluorescence” written by Piña et al. [...]
Reply to Sheval, E.V. Comment on “Piña et al. Ten Approaches That Improve Immunostaining: A Review of the Latest Advances for the Optimization of Immunofluorescence. Int. J. Mol. Sci. 2022, 23, 1426”
Authors: Piña Ricardo , Acosta-Galeana Isabel , Rosas-Arellano Abraham
Published: April 2022

We have carefully read the interesting explanatory comment by Eugene V. Sheval [...]
Computer Simulations and Network-Based Profiling of Binding and Allosteric Interactions of SARS-CoV-2 Spike Variant Complexes and the Host Receptor: Dissecting the Mechanistic Effects of the Delta and Omicron Mutations
Authors: Verkhivker Gennady , Agajanian Steve , Kassab Ryan , Krishnan Keerthi
Published: April 2022

In this study, we combine all-atom MD simulations and comprehensive mutational scanning of S-RBD complexes with the angiotensin-converting enzyme 2 (ACE2) host receptor in the native form as well as the S-RBD Delta and Omicron variants to (a) examine the differences in the dynamic signatures of the S-RBD complexes and (b) identify the critical binding hotspots and sensitivity of the mutational positions. We also examined the differences in allosteric interactions and communications in the S-RBD complexes for the Delta and Omicron variants. Through the perturbation-based scanning of the allosteric propensities of the SARS-CoV-2 S-RBD residues and dynamics-based network centrality and community analyses, we characterize the global mediating centers in the complexes and the nature of local stabilizing communities. We show that a constellation of mutational sites (G496S, Q498R, N501Y and Y505H) correspond to key binding energy hotspots and also contribute decisively to the key interfacial communities that mediate allosteric communications between S-RBD and ACE2. These Omicron mutations are responsible for both favorable local binding interactions and long-range allosteric interactions, providing key functional centers that mediate the high transmissibility of the virus. At the same time, our results show that other mutational sites could provide a “flexible shield” surrounding the stable community network, thereby allowing the Omicron virus to modulate immune evasion at different epitopes, while protecting the integrity of binding and allosteric interactions in the RBD–ACE2 complexes. This study suggests that the SARS-CoV-2 S protein may exploit the plasticity of the RBD to generate escape mutants, while engaging a small group of functional hotspots to mediate efficient local binding interactions and long-range allosteric communications with ACE2.
Modulation of Amyloid β-Induced Microglia Activation and Neuronal Cell Death by Curcumin and Analogues
Authors: De Lorenzi Ersilia , Franceschini Davide , Contardi Cecilia , Di Martino Rita Maria Concetta , Seghetti Francesca , Serra Massimo , Bisceglia Federica , Pagetta Andrea , Zusso Morena , Belluti Federica
Published: April 2022

Alzheimer’s disease (AD) is a progressive neurodegenerative disorder that is not restricted to the neuronal compartment but includes important interactions with immune cells, including microglia. Protein aggregates, common pathological hallmarks of AD, bind to pattern recognition receptors on microglia and trigger an inflammatory response, which contributes to disease progression and severity. In this context, curcumin is emerging as a potential drug candidate able to affect multiple key pathways implicated in AD, including neuroinflammation. Therefore, we studied the effect of curcumin and its structurally related analogues cur6 and cur16 on amyloid-β (Aβ)-induced microglia activation and neuronal cell death, as well as their effect on the modulation of Aβ aggregation. Primary cortical microglia and neurons were exposed to two different populations of Aβ42 oligomers (Aβ42Os) where the oligomeric state had been assigned by capillary electrophoresis and ultrafiltration. When stimulated with high molecular weight Aβ42Os, microglia released proinflammatory cytokines that led to early neuronal cell death. The studied compounds exerted an anti-inflammatory effect on high molecular weight Aβ42O-stimulated microglia and possibly inhibited microglia-mediated neuronal cell toxicity. Furthermore, the tested compounds demonstrated antioligomeric activity during the process of in vitro Aβ42 aggregation. These findings could be investigated further and used for the optimization of multipotent candidate molecules for AD treatment
Visualization of DNA Damage and Protection by Atomic Force Microscopy in Liquid
Authors: Dai Tinghui , Wang Yanwei , Yang Guangcan
Published: April 2022

DNA damage is closely related to cancer and many aging-related diseases. Peroxynitrite is a strong oxidant, thus a typical DNA damage agent, and is a major mediator of the inflammation-associated pathogenesis. For the first time, we directly visualized the process of DNA damage by peroxynitrite and DNA protection by ectoine via atomic force microscopy in liquid. We found that the persistence length of DNA decreases significantly by adding a small amount of peroxynitrite, but the observed DNA chains are still intact. Specifically, the persistence length of linear DNA in a low concentration of peroxynitrite (0 µM to 200 µM) solution decreases from about 47 nm to 4 nm. For circular plasmid DNA, we observed the enhanced superhelices of plasmid DNA due to the chain soften. When the concentration of peroxynitrite was above 300 µM, we observed the fragments of DNA. Interestingly, we also identified single-stranded DNAs during the damage process, which is also confirmed by ultraviolet spectroscopy. However, if we added 500 mM ectoine to the high concentration PN solution, almost no DNA fragments due to double strand breaks were observed because of the protection of ectoine. This protection is consistent with the similar effect for DNA damage caused by ionizing radiation and oxygenation. We ascribe DNA protection to the preferential hydration of ectoine.
Aconitate Decarboxylase 1 Deficiency Exacerbates Mouse Colitis Induced by Dextran Sodium Sulfate
Authors: Kim Ho Won , Yu A-Reum , Lee Ji Won , Yoon Hoe Sun , Lee Byung Soo , Park Hwan-Woo , Lee Sung Ki , Lee Young Ik , Whang Jake , Kim Jong-Seok
Published: April 2022

Ulcerative colitis is a complex inflammatory bowel disorder disease that can induce rectal and colonic dysfunction. Although the prevalence of IBD in Western countries is almost 0.5% of the general population, genetic causes are still not fully understood. In a recent discovery, itaconate was found to function as an immune-modulating metabolite in mammalian immune cells, wherein it is synthesized as an antimicrobial compound from the citric acid cycle intermediate cis-aconitic acid. However, the association between the Acod1 (Aconitate decarboxylase 1)-itaconate axis and ulcerative colitis has rarely been studied. To elucidate this, we established a DSS-induced colitis model with Acod1-deficient mice and then measured the mouse body weights, colon lengths, histological changes, and cytokines/chemokines in the colon. We first confirmed the upregulation of Acod1 RNA and protein expression levels in DSS-induced colitis. Then, we found that colitis symptoms, including weight loss, the disease activity index, and colon shortening, were worsened by the depletion of Acod1. In addition, the extent of intestinal epithelial barrier breakdown, the extent of immune cell infiltration, and the expression of proinflammatory cytokines and chemokines in Acod1-deficient mice were higher than those in wild-type mice. Finally, we confirmed that 4-octyl itaconate (4-OI) alleviated DSS-induced colitis in Acod1-deficient mice and decreased the expression of inflammatory cytokines and chemokines. To our knowledge, this study is the first to elucidate the role of the Acod1-itaconate axis in colitis. Our data clearly showed that Acod1 deletion resulted in severe DSS-induced colitis and substantial increases in inflammatory cytokine and chemokine levels. Our results suggest that Acod1 may normally play an important regulatory role in the pathogenesis of colitis, demonstrating the potential for novel therapies using 4-OI.
Add and Go: FRET Acceptor for Live-Cell Measurements Modulated by Externally Provided Ligand
Authors: Gavrikov Alexey S. , Bozhanova Nina G. , Baranov Mikhail S. , Mishin Alexander S.
Published: April 2022

A substantial number of genetically encoded fluorescent sensors rely on the changes in FRET efficiency between fluorescent cores, measured in ratiometric mode, with acceptor photobleaching or by changes in fluorescence lifetime. We report on a modulated FRET acceptor allowing for simplified one-channel FRET measurement based on a previously reported fluorogen-activating protein, DiB1. Upon the addition of the cell-permeable chromophore, the fluorescence of the donor-fluorescent protein mNeonGreen decreases, allowing for a simplified one-channel FRET measurement. The reported chemically modulated FRET acceptor is compatible with live-cell experiments and allows for prolonged time-lapse experiments with dynamic energy transfer evaluation.
Crystal Structure of an SSB Protein from Salmonella enterica and Its Inhibition by Flavanonol Taxifolin
Authors: Lin En-Shyh , Huang Yen-Hua , Luo Ren-Hong , Basharat Zarrin , Huang Cheng-Yang
Published: April 2022

Single-stranded DNA (ssDNA)-binding proteins (SSBs) play a central role in cells by participating in DNA metabolism, including replication, repair, recombination, and replication fork restart. SSBs are essential for cell survival and thus an attractive target for potential anti-pathogen chemotherapy. In this study, we determined the crystal structure and examined the size of the ssDNA-binding site of an SSB from Salmonella enterica serovar Typhimurium LT2 (SeSSB), a ubiquitous opportunistic pathogen which is highly resistant to antibiotics. The crystal structure was solved at a resolution of 2.8 Å (PDB ID 7F25), indicating that the SeSSB monomer possesses an oligonucleotide/oligosaccharide-binding (OB) fold domain at its N-terminus and a flexible tail at its C-terminus. The core of the OB-fold in the SeSSB is made of a six-stranded β-barrel capped by an α-helix. The crystal structure of the SeSSB contained two monomers per asymmetric unit, which may indicate the formation of a dimer. However, the gel-filtration chromatography analysis showed that the SeSSB forms a tetramer in solution. Through an electrophoretic mobility shift analysis, we characterized the stoichiometry of the SeSSB complexed with a series of ssDNA dA homopolymers, and the size of the ssDNA-binding site was determined to be around 22 nt. We also found the flavanonol taxifolin, also known as dihydroquercetin, capable of inhibiting the ssDNA-binding activity of the SeSSB. Thus, this result extended the SSB interactome to include taxifolin, a natural product with a wide range of promising pharmacological activities.
Linking Reactive Oxygen Species (ROS) to Abiotic and Biotic Feedbacks in Plant Microbiomes: The Dose Makes the Poison
Authors: Berrios Louis , Rentsch Jeremy D.
Published: April 2022

In nature, plants develop in complex, adaptive environments. Plants must therefore respond efficiently to environmental stressors to maintain homeostasis and enhance their fitness. Although many coordinated processes remain integral for achieving homeostasis and driving plant development, reactive oxygen species (ROS) function as critical, fast-acting orchestrators that link abiotic and biotic responses to plant homeostasis and development. In addition to the suite of enzymatic and non-enzymatic ROS processing pathways that plants possess, they also rely on their microbiota to buffer and maintain the oxidative window needed to balance anabolic and catabolic processes. Strong evidence has been communicated recently that links ROS regulation to the aggregated function(s) of commensal microbiota and plant-growth-promoting microbes. To date, many reports have put forth insightful syntheses that either detail ROS regulation across plant development (independent of plant microbiota) or examine abiotic–biotic feedbacks in plant microbiomes (independent of clear emphases on ROS regulation). Here we provide a novel synthesis that incorporates recent findings regarding ROS and plant development in the context of both microbiota regulation and plant-associated microbes. Specifically, we discuss various roles of ROS across plant development to strengthen the links between plant microbiome functioning and ROS regulation for both basic and applied research aims.
Inhibitory Response to CK II Inhibitor Silmitasertib and CDKs Inhibitor Dinaciclib Is Related to Genetic Differences in Pancreatic Ductal Adenocarcinoma Cell Lines
Authors: Ma Yixuan , Sender Sina , Sekora Anett , Kong Weibo , Bauer Peter , Ameziane Najim , Krake Susann , Radefeldt Mandy , Al-Ali Ruslan , Weiss Frank Ulrich , Lerch Markus M. , Parveen Alisha , Zechner Dietmar , Junghanss Christian , Murua Escobar Hugo
Published: April 2022

Casein kinase II (CK2) and cyclin-dependent kinases (CDKs) frequently interact within multiple pathways in pancreatic ductal adenocarcinoma (PDAC). Application of CK2- and CDK-inhibitors have been considered as a therapeutic option, but are currently not part of routine chemotherapy regimens. We investigated ten PDAC cell lines exposed to increasing concentrations of silmitasertib and dinaciclib. Cell proliferation, metabolic activity, biomass, and apoptosis/necrosis were evaluated, and bioinformatic clustering was used to classify cell lines into sensitive groups based on their response to inhibitors. Furthermore, whole exome sequencing (WES) and RNA sequencing (RNA-Seq) was conducted to assess recurrent mutations and the expression profile of inhibitor targets and genes frequently mutated in PDAC, respectively. Dinaciclib and silmitasertib demonstrated pronounced and limited cell line specific effects in cell death induction, respectively. WES revealed no genomic variants causing changes in the primary structure of the corresponding inhibitor target proteins. RNA-Seq demonstrated that the expression of all inhibitor target genes was higher in the PDAC cell lines compared to non-neoplastic pancreatic tissue. The observed differences in PDAC cell line sensitivity to silmitasertib or dinaciclib did not depend on target gene expression or the identified gene variants. For the PDAC hotspot genes kirsten rat sarcoma virus (KRAS) and tumor protein p53 (TP53), three and eight variants were identified, respectively. In conclusion, both inhibitors demonstrated in vitro efficacy on the PDAC cell lines. However, aberrations and expression of inhibitor target genes did not appear to affect the efficacy of the corresponding inhibitors. In addition, specific aberrations in TP53 and KRAS affected the efficacy of both inhibitors.