bluuebunny/crossref_metadata_2025 huggingface dataset:
Cervical Fluids Are a Source of Protein Biomarkers for Early, Non-Invasive Endometrial Cancer Diagnosis
Authors: Martinez-Garcia Elena , Coll-de la Rubia Eva , Lesur Antoine , Dittmar Gunnar , Gil-Moreno Antonio , Cabrera Silvia , Colas Eva
Published: February 2023

Background: Abnormal uterine bleeding is the main symptom of endometrial cancer (EC), but it is highly nonspecific. This represents a huge burden for women’s health since all women presenting with bleeding will undergo sequential invasive tests, which are avoidable for 90–95% of those women who do not have EC. Methods: This study aimed to evaluate the potential of cervical samples collected with five different devices as a source of protein biomarkers to diagnose EC. We evaluated the protein quantity and the proteome composition of five cervical sampling methods. Results: Samples collected with a Rovers Cervex Brush® and the HC2 DNA collection device, Digene, were the most suitable samples for EC proteomic studies. Most proteins found in uterine fluids were also detected in both cervical samples. We then conducted a clinical retrospective study to assess the expression of 52 EC-related proteins in 41 patients (22 EC; 19 non-EC), using targeted proteomics. We identified SERPINH1, VIM, TAGLN, PPIA, CSE1L, and CTNNB1 as potential protein biomarkers to discriminate between EC and symptomatic non-EC women with abnormal uterine bleeding in cervical fluids (AUC > 0.8). Conclusions: This study opens an avenue for developing non-invasive protein-based EC diagnostic tests, which will improve the standard of care for gynecological patients.
Tumour Growth Models of Breast Cancer for Evaluating Early Detection—A Summary and a Simulation Study
Authors: Strandberg Rickard , Abrahamsson Linda , Isheden Gabriel , Humphreys Keith
Published: February 2023

With the advent of nationwide mammography screening programmes, a number of natural history models of breast cancers have been developed and used to assess the effects of screening. The first half of this article provides an overview of a class of these models and describes how they can be used to study latent processes of tumour progression from observational data. The second half of the article describes a simulation study which applies a continuous growth model to illustrate how effects of extending the maximum age of the current Swedish screening programme from 74 to 80 can be evaluated. Compared to no screening, the current and extended programmes reduced breast cancer mortality by 18.5% and 21.7%, respectively. The proportion of screen-detected invasive cancers which were overdiagnosed was estimated to be 1.9% in the current programme and 2.9% in the extended programme. With the help of these breast cancer natural history models, we can better understand the latent processes, and better study the effects of breast cancer screening.
Syngeneic N1-S1 Orthotopic Hepatocellular Carcinoma in Sprague Dawley Rat for the Development of Interventional Oncology-Based Immunotherapy: Survival Assay and Tumor Immune Microenvironment
Authors: Choi Bongseo , Pe Jason , Yu Bo , Kim Dong-Hyun
Published: February 2023

Rodent HCC rat models provide advantages for interventional oncology (IO) based immunotherapy research compared to other established larger animal models or mice models. Rapid and predictable tumor growth and affordable costs permit the formation of a compelling preclinical model investigating novel IO catheter-directed therapies and local ablation therapies. Among orthotopic HCC models, the N1-S1 orthotopic HCC model has been involved in many research cases. Suboptimal tumor induction rates and potential spontaneous regression during tumor implantation procedures discouraged the use of the N1-S1 HCC model in IO-based immunotherapies. Here, N1-S1 HCC models were generated with a subcapsular implantation of two different number of N1-S1 cells using a mini-laporatomy. Tumor growth assay and immunological profiles which can preclinically evaluate the therapeutic efficacy of IO-based immunotherapy, were characterized. Finally, an N1-S1 HCC rat model generated with the proposed procedure demonstrated a representative immune suppressive HCC tumor environment without self-tumor regression. The optimized syngeneic N1-S1 HCC rat models represent an essential tool for pre-clinical evaluation of new IO immunotherapies for the treatment of HCC.
In Vivo Efficacy Testing of Peptide Receptor Radionuclide Therapy Radiosensitization Using Olaparib
Authors: Feijtel Danny , Reuvers Thom , van Tuyll-van Serooskerken Christine , de Ridder Corrina , Stuurman Debra , de Blois Erik , Verkaik Nicole , de Bruijn Peter , Koolen Stijn , de Jong Marion , Nonnekens Julie
Published: February 2023

Peptide receptor radionuclide therapy (PRRT), a form of internal targeted radiation treatment using [177Lu]Lu [DOTA0-Tyr3]octreotate, is used to treat patients with metastasized neuroendocrine tumors (NETs). Even though PRRT is now the second line of treatment for patients with metastasized NETs, the majority of patients will not be cured by the treatment. PRRT functions by inducing DNA damage upon radioactive decay and inhibition of DNA damage repair proteins could therefore be used as a strategy to potentiate PRRT. Previous work has shown promising results on the combination of PRRT with the PARP inhibitor olaparib in cell lines and mice and we have been taken the next step for further in vivo validation using two different xenografted mouse models. We observed that this combination therapy resulted in increased therapeutic efficacy only in one model and not the other. Overall, our findings indicate a tumor-type dependent anti-tumor response to the combination of PRRT and olaparib. These data emphasize the unmet need for the molecular stratification of tumors to predetermine the potential clinical value of combining PARP inhibition with PRRT.
Dynamic Changes in Microvascular Density Can Predict Viable and Non-Viable Areas in High-Risk Neuroblastoma
Authors: Privitera Laura , Musleh Layla , Paraboschi Irene , Ogunlade Olumide , Ogunbiyi Olumide , Hutchinson J. Ciaran , Sebire Neil , Beard Paul , Giuliani Stefano
Published: February 2023

Despite aggressive treatments, the prognosis of high-risk NB remains poor. Surgical oncology needs innovative intraoperative devices to help surgeons discriminate malignant tissue from necrotic and surrounding healthy tissues. Changes within the tumor vasculature could be used intraoperatively as a diagnostic tool to guide surgical resection. Here, we retrospectively analyzed the mean vascular density (MVD) of different NB subtypes at diagnosis and after induction chemotherapy using scanned histological samples. One patient was prospectively enrolled, and an ex vivo photoacoustic imaging (PAI) scan was performed on two representative sections to assess its capacity to discriminate different tumor regions. We found that post-chemotherapy, viable areas of differentiating NBs and ganglioneuroblastomas are associated with higher MVD compared to poorly differentiated NBs. Early necrotic regions showed higher MVD than late necrotic and viable regions. Finally, calcified areas showed significantly lower MVD than any other histological component. The acquired PAI images showed a good high-resolution ex vivo 3D delineation of NB margins. Overall, these results suggest that a high-definition preclinical imaging device such as PAI could potentially be exploited to guide surgical resection by identifying different vasculature signatures.
Safety and Efficacy of Sildenafil for Group 2 Pulmonary Hypertension in Left Heart Failure
Authors: Desai Kinjal , Di Lorenzo Michael , Zuckerman Warren A. , Emeruwa Ezinne , Krishnan Usha S.
Published: February 2023

Pulmonary hypertension (PH) is a multifactorial, progressive disease with poor outcomes. Group 2 PH is defined by pulmonary vascular disease with elevated pulmonary capillary wedge pressure including both left-sided obstructive lesions and diastolic heart failure (HF). Sildenafil was historically discouraged in this population as pulmonary vasodilation can lead to pulmonary edema. However, evidence suggests that sildenafil can help to treat the precapillary component of PH. This is a single center, retrospective pilot study of pediatric PH patients with left-sided HF who were treated with sildenafil for ≥ 4 weeks. HF patients without mechanical support (HF group) and HF patients with a left ventricular assist device (HF-VAD) were analyzed. The exploratory analysis described the safety and side effects of the drug. Echocardiographic parameters were compared before and after sildenafil treatment in a paired analysis. The changes in medical therapy during treatment, mechanical support, and mortality was reported; 19/22 patients tolerated sildenafil. Pulmonary edema in two patients resolved upon discontinuation of sildenafil. In the HF group, both the right atrial volume and right ventricular diastolic area decreased, and the tricuspid regurgitation (TR) S/D ratio decreased after therapy (p = 0.02). Across both the groups, four patients weaned off milrinone and seven weaned off inhaled nitric oxide. Of the thirteen HF patients, four received a transplant, and all of the nine HF-VAD patients received a transplant. Sildenafil can be safely used in carefully selected patients with HF and mixed pre/postcapillary PH with judicious titration and inpatient surveillance, with patients showing improvements in echocardiographic parameters.
Social Media in Adolescents: A Retrospective Correlational Study on Addiction
Authors: Ciacchini Rebecca , Orrù Graziella , Cucurnia Elisa , Sabbatini Silvia , Scafuto Francesca , Lazzarelli Alessandro , Miccoli Mario , Gemignani Angelo , Conversano Ciro
Published: February 2023

Considering the growing interest in the possible effects of internet’s addiction on adoles-cent’s mental health, this study aimed at exploring the psychological correlates of social media and internet problematic use during the first year of the covid-19 pandemic. A cross-sectional study was conducted in a sample of secondary school students (N = 258); participants were asked to complete an online survey, investigating social media addiction (BSMAS), self-esteem (RSES), feelings of isolation (CSIQ-A) and anxiety (STAI-Y). Data analysis (descriptive statistics, correlational and regression analyses) was conducted through XLSTAT software ©. An additional ad hoc questionnaire was administrated. Findings showed that the 11% of the participants were significantly addicted to social media, mostly females (59%). Gender represented an exposure factor for the hours spent on social media and the checking activity while performing other daily activities. Significant correlations emerged between the self-report measure of social media addiction and self-esteem and anxiety. Low scores at RSES corresponded to higher checking activity, hours spent on social networks, and playing videogames that were investigated as supplementary indicators of addiction with ad hoc questionnaire. The regression analysis showed just two predictors of social media addiction, gender (female) and trait anxiety. Limitations and implications of the study were argued in order to give some indications for future programs.
Partial Remodeling after Conservative Treatment of Trampoline Fractures in Children
Authors: Zaccaria Laura , Stranzinger Enno , Xydias Theodoros , Schaedelin Sabine , Ziebarth Kai , Trück Mike , Sommer-Joergensen Vivienne , Aufdenblatten Christoph , Klimek Peter Michael
Published: February 2023

(1) Background: Trampoline fractures (proximal tibia fracture with positive anterior tilt) are increasing. This study represents the first attempt to determine the extent of remodeling in these fractures after conservative treatment (2) Methods: This Swiss prospective multicenter study included children aged 2 to 5 years with a trampoline fracture who were radiologically examined on the day of the accident and after one year. In addition, the anterior tilt angle was compared between the injured and unaffected tibia. Remodeling was defined as complete (final anterior tilt angle ≤ 0°), incomplete (smaller but still >0°), or no remodeling. (3) Results: The mean extent of remodeling was −3.5° (95% CI: −4.29°, −2.66°, p < 0.001). Among the 89 children included in the study, 26 (29.2%) showed complete, 63 (70.8%) incomplete, and 17 patients (19.1%) no remodeling. Comparison of the anterior tilt angles between the fractured and healthy tibia showed that the anterior tilt angle on the fractured leg was, on average larger by 2.82° (95% CI: 2.01°, 3.63°; p < 0.001). (4) Conclusions: Although the anterior tilt angle decreased during the study period, the majority of patients showed incomplete remodeling. In contrast, children with radiological examinations >1 year after the trauma showed advanced remodeling, suggesting that one year is too short to observe complete remodeling.
Four Different Finger Positions and Their Effects on Hemodynamic Changes during Chest Compression in Asphyxiated Neonatal Piglets
Authors: Bruckner Marlies , Neset Mattias , O’Reilly Megan , Lee Tze-Fun , Cheung Po-Yin , Schmölzer Georg M.
Published: February 2023

Background: The Neonatal Life Support Consensus on Science With Treatment Recommendations states that chest compressions (CC) be performed preferably with the 2-thumb encircling technique. The aim of this study was to compare the hemodynamic effects of four different finger positions during CC in a piglet model of neonatal asphyxia. Methods: Seven asphyxiated post-transitional piglets were randomized to CC with 2-thumb-, 2-finger-, knocking-fingers-, and over-the-head 2-thumb-techniques for one minute at each technique. CC superimposed with sustained inflations were performed manually. Results: Seven newborn piglets (age 0–4 days, weight 2.0–2.1 kg) were included in the study. The mean (SD) slope rise of carotid blood flow was significantly higher with the 2-thumb-technique and over-the-head 2-thumb-technique (118 (45) mL/min/s and 121 (46) mL/min/s, respectively) compared to the 2-finger-technique and knocking-finger-technique (75 (48) mL/min/s and 71 (67) mL/min/s, respectively) (p < 0.001). The mean (SD) dp/dtmin (as an expression of left ventricular function) was significantly lower with the 2-thumb-technique, with −1052 (369) mmHg/s, compared to −568 (229) mmHg/s and −578(180) mmHg/s (both p = 0.012) with the 2-finger-technique and knocking-finger-technique, respectively. Conclusion: The 2-thumb-technique and the over-the-head 2-thumb-technique resulted in improved slope rises of carotid blood flow and dp/dtmin during chest compression.
Comparison between Restorative Materials for Pulpotomised Deciduous Molars: A Randomized Clinical Study
Authors: Kaur Kanwalpreet , Suneja Bharat , Jodhka Sunaina , Saini Ravinder S. , Chaturvedi Saurabh , Bavabeedu Shashit Shetty , Alhamoudi Fahad Hussain , Cicciù Marco , Minervini Giuseppe
Published: February 2023

Aim: To evaluate and compare the clinical outcomes of Cention-N (CN) and stainless steel crowns (SSCs) as restorations for pulpotomised primary molars, and to study clinical and radiographic outcomes of pulpotomies restored with these materials. Methods: The study was conducted on 60 pulpotomised molars with occlusoproximal caries. These were randomly divided into two groups and restored with either stainless steel crowns or Cention-N. Clinical performance of restorations and clinical and radiographic success of pulpotomy was examined at 6, 9 and 12 months. Results: The mean scores for marginal integrity deteriorated significantly at 6, 9 and 12 months in both groups but in comparison were insignificant. The mean for proximal contact deteriorated significantly for the Cention-N group, whereas the mean for gingival health deteriorated remarkably for the stainless steel crown group at successive evaluations. No tooth in either group showed secondary caries or discomfort on biting, except for one tooth in Cention-N group which presented with secondary caries. The clinical success rate for pulpotomised molars was 100% for both groups until nine months, although this had reduced by the end of 12 months. Radiographically, the success rate was 79.3% for Cention-N, while it was 86.6% for stainless steel crowns at 12 months. There was no significant difference in clinical and radiographic success between either group. Conclusion: Cention-N and stainless steel crowns are comparable for marginal integrity. However, crowns maintain significantly better proximal contacts while Cention-N was notably better for gingival health of the restored tooth. Both materials do not show secondary caries and discomfort on biting and are comparable in clinical and radiographic success of pulpotomy at the end of one year.
Psychiatric Disorders and Obesity in Childhood and Adolescence—A Systematic Review of Cross-Sectional Studies
Authors: Kokka Ioulia , Mourikis Iraklis , Bacopoulou Flora
Published: February 2023

Obesity and psychiatric disorders have high prevalence and are both considered major health problems. Within the last decades, the rates of obesity have risen over 6%, while the prevalence of psychiatric disorders is over 12% for children and adolescents. The aim of this study was to systematically review the evidence regarding the relation of obesity and psychiatric disorders in childhood and adolescence. This review, based on the PRISMA guidelines, included cross-sectional studies published within the last decade, pertaining to the relation between psychiatric disorders and obesity in children and adolescents up to the age of 19 years. Studies on eating disorders were excluded. A total of 14 studies of 23,442 children and adolescents that investigated the relation of obesity with anxiety, mood disorders, and psychosis were included in this systematic review. Nine of the included studies reported a significant relationship between the psychiatric disorder under investigation and obesity. Understanding the nexus between obesity and psychiatric disorders in children and adolescents is of great importance, given the alarming increase in both conditions in youth. Such findings could facilitate the development and implementation of targeted interventions.
Development of an In Situ Micro-Corrosion Cell for the Investigation of Pitting Corrosion on Austenitic and Ferritic Stainless Steels
Authors: Kiremit Sinan , Cremer Julian , Stallmeier Yannic , Sonntag Adrian , Klöcker Michaela , Anselmetti Dario , Hütten Andreas , Kordisch Thomas
Published: February 2023

In order to investigate the electrochemical pitting corrosion in more detail, a micro-corrosion cell was developed, allowing real-time in situ optical observations of steel surfaces in direct correlation with electrochemical measurement results. In this study, the austenitic 1.4301–X5CrNi18-10 and the ferritic 1.4016–X6Cr17 stainless steel grades were examined in electrolytes containing chloride ions. The micro-corrosion cell revealed a stable pitting corrosion of the ferritic 1.4016 and metastable pitting corrosion of the austenitic 1.4301. The pits were characterized by means of scanning electron microscopy (SEM) and atomic force microscopy (AFM) in detail. A clear correlation between current peaks in the current density-potential curve and the growth of many small pits on the test surface was established and was identified as metastable pit growth. In general, the pitting corrosion potential increased as the diameter of the test surface decreased for both stainless steels. In contrast to the complex precipitates of 1.4301, chromium precipitates with a significantly higher amount was detected on the entire surface of the 1.4016. The corrosion initiation was identified at the interface between the precipitates and the base material for both stainless steels. By comparing both materials, the ferritic 1.4016 had a lower pitting corrosion potential than the austenitic 1.4301 under all test conditions.
The Hell of Wildfires: The Impact on Wildlife and Its Conservation and the Role of the Veterinarian
Authors: Garcês Andreia , Pires Isabel
Published: February 2023

Wildfires are common occurrences worldwide that can destroy vast forest areas and kill numerous animals in a few hours. Climate change, rising global temperatures, precipitation, the introduction of exotic species of plants (e.g., eucalyptus), intensive agriculture, and deforestation have increased the number of fires and their intensity and destructive power. Nearly 4% of the global land surface (30–46 million/km2) is burned annually. There are many studies regarding the impact of wildfires on ecosystems, flora, domestic animals, and humans. Even though wildfires are a common and recurrent problem around the world, most of the time, they are a neglected issue, especially regarding wildlife. The information available is scarce and dispersed across several bibliographical references, and the veterinarian teams, most of the time, need to be trained to act in these situations. In this brief review, we describe different species’ behavior during a wildfire, the effects on the ecosystem, and the socioeconomic impact on the region. From a veterinarian’s perspective, we list the major injuries that are expected to be observed and how to proceed. In conclusion, we discuss better prevention and response measures in a wildfire scenario. This information can be used by veterinarians and all the entities involved in the prevention and combat of wildfires, and the general public has an important role in these situations.
The Facet of Human Impact: Solenopsis invicta Buren, 1972 Spreading around the Atlantic Forest
Authors: Ramalho Manuela O. , Nagatani Victor H. , Alves Juliana M. C. , Silva Otavio G. M. , Fox Eduardo G. P. , Souza Rodrigo F. , Kayano Debora Y. , Harakava Ricardo , Hilsdorf Alexandre W. S. , Morini Maria S. C.
Published: February 2023

The present investigation deals with some aspects of the diversity of fire ants (Hymenoptera: Formicidae) in their native range. The Red Imported Fire Ant Solenopsis invicta is native to the tropical and subtropical inland territories of South America. In Brazil, it mainly occurs around the Pantanal region and across the Paraguay river, a region composed of grasslands which are seasonally flooded. Recent studies have evidenced this fire ant species is gradually spreading to other regions of Brazil. In the present investigation, we surveyed the molecular diversity of S. invicta populations across fragments of Atlantic Forest in São Paulo, Brazil, using mtDNA COI haplotypes. Fire ant nests were sampled along the highways lining the northern and southern slope sides of the mountain range Serra do Mar, SP, Brazil. Four haplotypes were identified (H1–H4), which were assessed for similarity to deposited records by other authors, revealing that the haplotypes H1 and H2 are likely of foreign origin through recent reintroduction via a marine port to the south of the Serra do Mar mountain range. On the other hand, the haplotypes H3 and H4, predominating among the inland samples from the northern side of the mountain range, were most similar to previous records from more central regions of Brazil. Haplotypes clustered into distinct supergroups, further pointing to the occurrence of two separate expansion waves of S. invicta in the region. We suggest the obtained pattern indicates the mountain range may function as a geographical barrier deferring gene flow.
Seed Morphology, Life Form and Distribution in Three Bromheadia Species (Epidendroideae, Orchidaceae)
Authors: Ortúñez Emma , Gamarra Roberto
Published: February 2023

The seed morphology of three species belonging to the genus Bromheadia was analyzed under light and scanning electron microscopy. The seeds of B. cecieliae and B. truncata were studied for the first time. Differences in the qualitative and quantitative characteristics between the terrestrial B. finlaysoniana and the epiphytes B. cecieliae and B. truncata were observed, which were in concordance with the life form. Due to the variability of the seed shapes, a new methodology is proposed to analyze the distance between the embryo and the testa cells, with the aim of demonstrating the presence of air space within the seed. The method is compared to previous formulae used to measure free air space. Furthermore, a new measurement, the angle in twisted testa cells of epiphytic orchids, is proposed, to evaluate the degree of torsion in medial cells. Although the wide distribution of B. finlaysoniana could be related to the great buoyancy of their seeds in contrast to the limited distribution of B. cecieliae, we consider that environmental factors are more influential than the buoyancy of seeds when understanding the distribution of these taxa. Future studies on seeds morphology in orchid genera with terrestrial and epiphytic taxa will provide new insights into this research.
PFOS Induces Lipometabolism Change, Immune Defense, and Endocrine Disorders in Black-Spotted Frogs: Application of Transcriptome Profiling
Authors: Shi Chaoli , Yang Hongmei , Xu Mengxuan , Hua Tianyang , He Mengfan , Yang Yuchen , Hou Xiaoyu , Zhang Hangjun , Liu Zhiquan
Published: February 2023

Amphibian population declines are closely linked to increasingly serious environmental pollution. Field investigations revealed that perfluorooctane sulfonic acid (PFOS) distribution was detected in 100% of amphibians. In the present study, global transcriptome sequencing was determined on black-spotted frogs to quantify transcript expression levels and the development of an adverse outcome pathway for PFOS. A total of 1441 differentially expressed genes were identified in the PFOS exposure for 21 d, with 645 being downregulated and 796 upregulated. The gene functions and pathways for lipid metabolism, endocrine system, and immune defense were enriched. An adverse outcome pathway has been proposed, including PPAR (peroxisome proliferator-activated receptors) as the molecular initiating events; followed by changes in lipid metabolism, endocrine system, and immune defense; with an end result of liver damage or even population decline. This research provides molecular insight into the toxicity of PFOS. More research about differentially expressed genes is warranted to further provide the underlying mechanism that is altered as a result of PFOS toxicity in organisms.
Structural Organization of Zooplankton Communities in Different Types of River Mouth Areas
Authors: Zhikharev Vyacheslav , Gavrilko Dmitry , Kudrin Ivan , Vodeneeva Ekaterina , Erina Oksana , Tereshina Maria , Shurganova Galina
Published: February 2023

The gradients of hydrological, hydrophysical, and hydrochemical conditions form the transition zones in river mouth areas. These areas are considered ecotones in the presence of the edge effect. Our research aimed to identify the main patterns in the structural organization of zooplankton communities in different types of river mouth areas in lowland reservoir tributaries within the Middle Volga basin. A cluster analysis was performed to structure and determine the zooplankton communities. The identified zooplankton communities were characterized by the noticeable heterogeneity in the species structure. We analyzed the spatial distribution of the structural indicators of zooplankton communities and, on the basis of RDA, determined the factors that significantly affect the organization of the ecotone community. An ecotone was found in all types of river mouth areas where the highest density, biomass, diversity, species, and functional richness of zooplankton were noted, as compared to bordering zooplankton communities. As the morphological structure of the mouth areas became more complex, the values of the main structural parameters of zooplankton communities increased. It was demonstrated that conditions in the ecotones are favorable to the development of zooplankton alien species. The major factors determining the species structure of the ecotone zooplankton community were water electrical conductivity (R2 = 21.07%, p-value = 0.001), dissolved oxygen content (R2 = 10.33%, p-value = 0.003), and water transparency (R2 = 9.77 %, p-value = 0.001).
Volatiles Emitted by Three Genovese Basil Cultivars in Different Growing Systems and Successive Harvests
Authors: Ciriello Michele , Formisano Luigi , Rouphael Youssef , Corrado Giandomenico
Published: February 2023

The Genovese basil (Ocimum basilicum L.) is the essential ingredient in “pesto” sauce, and it has always had ample use in Mediterranean gastronomy. This horticultural type of basil is grown in the open field and harvested more than once during its cultivation cycle, but in recent decades it is increasingly grown using alternative cultivation methods (e.g., soilless cultivation) that guarantee higher and more uniform production. The dataset presented in this contribution refers to the analysis of the aroma profile by solid-phase microextraction and gas chromatography coupled with a mass spectrometer, of three different cultivars of Genovese basil (Aroma 2, Eleonora, and Italiano Classico) grown in the open field or floating raft system in two successive harvests. The data are a record of the variability of volatile organic compounds due to key agronomic factors, such as the genotype, the cultivation method, and the cut. They may be of interest for those concerned about the impact of different technical factors on the aroma and flavor of basil plants.
Bio-lubricant Properties Analysis of Drilling an Innovative Design of Bioactive Kinetic Screw into Bone
Authors: Andreucci Carlos Aurelio , Fonseca Elza M. M. , Jorge Renato N.
Published: February 2023

Biotribology is applied to study the friction, wear, and lubrication of biological systems or natural phenomena under relative motion in the human body. It is a multidisciplinary field and tribological processes impact all aspects of our daily life. Tribological processes may occur after the implantation of an artificial device in the human body with a wide variety of sliding and frictional interfaces. Blood is a natural bio-lubricant experiencing laminar flow at the lower screw velocities associated with drilling implants into bone, being a viscoelastic fluid with viscous and fluid characteristics. The viscosity comes from the blood plasma, while the elastic properties are from the deformation of red blood cells. In this study, drilling parameters according to material properties obtained by Finite Element Analysis are given. The influence of blood on the resulting friction between the surfaces is demonstrated and correlated with mechanical and biological consequences, identifying an innovative approach to obtaining a new lubricant parameter for bone drilling analysis. The lubrication parameter (HN) found within the limitations of conditions used in this study is 10.7 × 10−7 for both cortical bone (D1) and spongy bone (D4). A thermal-structural analysis of the densities of the soft bone (D4) and hard bone (D1) shows differences in only the equivalent stress values due to the differences in respective Young moduli. The natural occurrences of blood as a lubricant in bone-screw perforations are poorly investigated in the literature and its effects are fundamental in osseointegration. This work aims to elucidate the relevance of the study of blood as a lubricant in drilling and screwing implants into bone at lower speeds.
Design of an Optically Accessible Intake Manifold for Characterization of Liquid and Gaseous Jets in PFI Operating Conditions
Authors: Cecere Giovanni , Irimescu Adrian , Merola Simona Silvia
Published: February 2023

The intake manifold and its components play a key role in the proper formation of air–fuel mixtures suitable for correct engine operation. In this article, starting from the original intake manifold design fitted to an optically accessible spark-ignited engine, a new solution was developed so as to allow the application of high-speed imaging of the fuel jet located between the runner and intake valves (Port Fuel Injection). To compare the two designs in terms of overall engine performance parameters such as volumetric efficiency, 0D/1D simulations were performed in motored conditions. Measurements at different crankshaft speed values were used for calibrating the intake line parameters and providing boundary conditions. Finite Element Analysis (FEM) was performed in SolidWorks to verify the structural strength of the new design when operating in the most critical conditions, i.e., boosted operation. As an overall conclusion, the results show that the new design guarantees a wider range of intake pressure values during the intake stroke, thus expanding the possible operative points. This can be obtained without compromising structural integrity, given that predicted safety factors were well above acceptable limits even for relatively high boost levels.
Fabrication of Graphene Oxide-Decorated Mesoporous NiFe2O4 as an Electrocatalyst in the Hydrogen Gas Evolution Reaction
Authors: Sanjaya Afiten R. , Amanda Salsabila , Ivandini Tribidasari A. , Abnisa Faisal , Kadja Grandprix T. M. , Pratomo Uji , Alias Yatimah , Khalil Munawar
Published: February 2023

An electrocatalyst for the hydrogen evolution reaction has been successfully synthesized from graphene oxide (GO) decorated with the mesoporous NiFe2O4. A high catalytic activity performance was reached by using the GCE surface as a conductor, and the synthesized composite contained GO/NiFe2O4. Based on the results, the as-prepared electrocatalyst exhibited a high overpotential for the HER reaction of 36 mV vs. RHE at a 10 mA current density, with an electrochemical active surface area (ECSA) of 3.18 × 10−4 cm2. Additionally, the electrocatalyst demonstrated a considerably good performance after the 9000 s stability test. It is believed that such an enhancement in electrocatalytic activity was due to the synergistic effect between the unique porosity feature of the mesoporous NiFe2O4, which may provide a more active surface, and the high conductivity of the GO.
Clinical Application of High-Frame-Rate Vector Flow Imaging in Evaluation of Carotid Atherosclerotic Stenosis
Authors: Qiu Yi-Jie , Cheng Juan , Zhang Qi , Yang Dao-Hui , Zuo Dan , Mao Feng , Liu Ling-Xiao , Dong Yi , Cao Si-Qi , Wang Wen-Ping
Published: February 2023

Objective: This study seeks to evaluate the value of the high-frame-rate vector flow imaging technique in assessing the hemodynamic changes of carotid atherosclerotic stenosis in aging people (>60 years old). Methods: Aging patients diagnosed with carotid atherosclerotic stenosis who underwent carotid high-frame-rate vector flow imaging examination were prospectively enrolled. A Mindray Resona7s ultrasound machine equipped with high-frame-rate vector flow function was used for ultrasound evaluation. First, B mode ultrasound and color Doppler flow imaging were used to evaluate carotid stenosis. Then, the vector arrows and flow streamline detected by V Flow were analyzed and the wall shear stress values (Pa) at the carotid stenosis site were measured. All patients were divided into symptomatic and asymptomatic groups according to whether they had acute/subacute stroke or other clinical symptoms within 2 weeks before ultrasound examination. The results of digital subtraction angiography or computed tomography angiography were used as the gold standard. The stenosis rate was calcified, according to North American Symptomatic Carotid Endarterectomy Trial criteria. The diagnostic values of wall shear stress, conventional ultrasound, and the combined diagnosis in carotid atherosclerotic stenosis were compared. Results: Finally, 88 patients with carotid atherosclerotic plaque were enrolled (71 males (80.7%), mean age 67.6 ± 5.4 years). The success rate of high-frame-rate vector flow imaging was 96.7% (88/91). The WSS value of symptomatic carotid stenosis (1.4 ± 0.15 Pa) was significantly higher than that of asymptomatic carotid stenosis (0.80 ± 0.08 Pa) (p < 0.05). Taking the wall shear stress value > 0.78 Pa as the diagnostic criteria for symptomatic carotid atherosclerotic plaque, the area under receiver operating characteristic curves was 0.79 with 87.1% sensitivity and 69.6% specificity. The area under receiver operating characteristic curves of the combined diagnosis (0.966) for differentiating severe carotid atherosclerotic stenosis was significantly higher than that of conventional ultrasound and WSS value, with 89.7% sensitivity and 93.2% specificity (p < 0.05). Conclusion: As a non-invasive imaging method, the high-frame-rate vector flow imaging technique showed potential value in the preoperative assessment of the symptomatic carotid atherosclerotic stenosis and diagnosing carotid atherosclerotic stenosis in aging patients.
Robust Superpixel Segmentation for Hyperspectral-Image Restoration
Authors: Fan Ya-Ru
Published: February 2023

Hyperspectral-image (HSI) restoration plays an essential role in remote sensing image processing. Recently, superpixel segmentation-based the low-rank regularized methods for HSI restoration have shown outstanding performance. However, most of them simply segment the HSI according to its first principal component, which is suboptimal. In this paper, integrating the superpixel segmentation with principal component analysis, we propose a robust superpixel segmentation strategy to better divide the HSI, which can further enhance the low-rank attribute of the HSI. To better employ the low-rank attribute, the weighted nuclear norm by three types of weighting is proposed to efficiently remove the mixed noise in degraded HSI. Experiments conducted on simulated and real HSI data verify the performance of the proposed method for HSI restoration.
On the Fitness Functions Involved in Genetic Algorithms and the Cryptanalysis of Block Ciphers
Authors: Tito-Corrioso Osmani , Borges-Quintana Mijail , Borges-Trenard Miguel A. , Rojas Omar , Sosa-Gómez Guillermo
Published: February 2023

There are many algorithms used with different purposes in the area of cryptography. Amongst these, Genetic Algorithms have been used, particularly in the cryptanalysis of block ciphers. Interest in the use of and research on such algorithms has increased lately, with a special focus on the analysis and improvement of the properties and characteristics of these algorithms. In this way, the present work focuses on studying the fitness functions involved in Genetic Algorithms. First, a methodology was proposed to verify that the closeness to 1 of some fitness functions’ values that use decimal distance implies decimal closeness to the key. On the other hand, the foundation of a theory is developed in order to characterize such fitness functions and determine, a priori, if one method is more effective than another in the attack to block ciphers using Genetic Algorithms.
Inferring Cultural Landscapes with the Inverse Ising Model
Authors: Poulsen Victor Møller , DeDeo Simon
Published: February 2023

The space of possible human cultures is vast, but some cultural configurations are more consistent with cognitive and social constraints than others. This leads to a “landscape” of possibilities that our species has explored over millennia of cultural evolution. However, what does this fitness landscape, which constrains and guides cultural evolution, look like? The machine-learning algorithms that can answer these questions are typically developed for large-scale datasets. Applications to the sparse, inconsistent, and incomplete data found in the historical record have received less attention, and standard recommendations can lead to bias against marginalized, under-studied, or minority cultures. We show how to adapt the minimum probability flow algorithm and the Inverse Ising model, a physics-inspired workhorse of machine learning, to the challenge. A series of natural extensions—including dynamical estimation of missing data, and cross-validation with regularization—enables reliable reconstruction of the underlying constraints. We demonstrate our methods on a curated subset of the Database of Religious History: records from 407 religious groups throughout human history, ranging from the Bronze Age to the present day. This reveals a complex, rugged, landscape, with both sharp, well-defined peaks where state-endorsed religions tend to concentrate, and diffuse cultural floodplains where evangelical religions, non-state spiritual practices, and mystery religions can be found.
Records and Occupation Time Statistics for Area-Preserving Maps
Authors: Artuso Roberto , de Oliveira Tulio M. , Manchein Cesar
Published: February 2023

A relevant problem in dynamics is to characterize how deterministic systems may exhibit features typically associated with stochastic processes. A widely studied example is the study of (normal or anomalous) transport properties for deterministic systems on non-compact phase space. We consider here two examples of area-preserving maps: the Chirikov–Taylor standard map and the Casati–Prosen triangle map, and we investigate transport properties, records statistics, and occupation time statistics. Our results confirm and expand known results for the standard map: when a chaotic sea is present, transport is diffusive, and records statistics and the fraction of occupation time in the positive half-axis reproduce the laws for simple symmetric random walks. In the case of the triangle map, we retrieve the previously observed anomalous transport, and we show that records statistics exhibit similar anomalies. When we investigate occupation time statistics and persistence probabilities, our numerical experiments are compatible with a generalized arcsine law and transient behavior of the dynamics.
Development of Longwall Shearers’ Haulage Systems as an Alternative to the Eicotrack System Used Nowadays
Authors: Kotwica Krzysztof , Stopka Grzegorz , Wieczorek Andrzej N. , Kalita Marek , Bałaga Dominik , Siegmund Michał
Published: February 2023

Longwall shearers’ haulage system of the Eicotrack type, used most often nowadays, is presented in this article. Its disadvantages, causing problems with a correct operation of mechanized longwall shearer systems, are discussed. The concept of the innovative Flextrack, which should reduce the occurrence of the disadvantages mentioned above, is described. A course and research results, connected with rig tests of the Flextrack haulage system functionality, are presented. Measurement results of wear and stresses, obtained for the Eicotrack and Flextrack haulage systems, are compared and presented. Based on an analysis of the obtained results, a modified version of the Komtrack haulage system was suggested and manufactured. This haulage system was tested in the field conditions, similar to underground conditions in a mine coal longwall. The obtained results confirmed its full functionality and lack of problems experienced in the case of the Eicotrack and Flextrack systems. At present, the Komtrack system is tested in a coal longwall in Piast coal mine, where comparative tests with the Eicotrack system are conducted. The big part of the results presented in this article was developed as part of the research project KOMTRACK jointly implemented by KOMAG Institute of Mining Technology, AGH University of Science and Technology, Łukasiewicz Research Network—Cracow Institute of Technology, Specodlew Innovative Foundry Company and Polish Mining Group Inc., co-financed by the European Regional Development Fund (Contract No. POIR.04.01.04-00-0068/17).
Small-Scale Mechanical Recycling of Solid Thermoplastic Wastes: A Review of PET, PEs, and PP
Authors: Uzosike Canice C. , Yee Lachlan H. , Padilla Ricardo Vasquez
Published: February 2023

The mechanical recycling of solid plastic waste on a small-scale level can be accomplished with the correct approaches. Thermoplastics are the types of plastic mostly considered for mechanical recycling because of their physical properties and ease of reprocessing. This paper reviews the mechanical reprocessing techniques of selected thermoplastics (polyethylene terephthalate and polyolefins), since they constitute a significant proportion of the plastics used commercially. Furthermore, necessary considerations for the effective operation of small-scale plants, including energy requirements of machinery and optimisation in order to improve efficiency and product quality, are discussed. A clearer understanding and addressing of the process-related challenges will lead to the successful establishment and management of small-scale mechanical recycling facilities to benefit communities. Efficient small-scale mechanical reprocessing establishments have become essential in reducing the environmental impacts of solid plastic waste and for energy conservation.
Navier-Stokes Solutions for Accelerating Pipe Flow—A Review of Analytical Models
Authors: Urbanowicz Kamil , Bergant Anton , Stosiak Michał , Deptuła Adam , Karpenko Mykola
Published: February 2023

This paper reviews analytical solutions for the accelerated flow of an incompressible Newtonian fluid in a pipeline. This problem can be solved in one of two ways according to the (1) imposed pressure gradient or (2) flow rate. Laminar accelerated flow solutions presented in a number of publications concern cases where the two driving mechanisms are described by simple mathematical functions: (a) impulsive change; (b) constant change; (c) ramp change, etc. The adoption of a more complex and realistic description of the pressure gradient or flow rate will be associated with a profound mathematical complexity of the final solution. This is particularly visible with the help of the universal formula derived by several researchers over the years and discussed in this paper. In addition to the solutions strictly defined for laminar flow, an interesting extension of this theory is the theory of underlying laminar flow for the analysis of turbulent accelerated pipe flows (TULF model developed by García García and Alvariño). The TULF model extends the Pai model developed more than 60 years ago, which has been previously used for steady flows only. The discussed solutions extend the theory of analytical solutions of simplified two-dimensional Navier–Stokes equations and can be used not only to study the behavior of liquids during accelerating pipe flow but they can also be used to test the accuracy of commercial CFD codes.
Classification of Wear State for a Positive Displacement Pump Using Deep Machine Learning
Authors: Konieczny Jarosław , Łatas Waldemar , Stojek Jerzy
Published: February 2023

Hydraulic power systems are commonly used in heavy industry (usually highly energy-intensive) and are often associated with high power losses. Designing a suitable system to allow an early assessment of the wear conditions of components in a hydraulic system (e.g., an axial piston pump) can effectively contribute to reducing energy losses during use. This paper presents the application of a deep machine learning system to determine the efficiency state of a multi-piston positive displacement pump. Such pumps are significant in high-power hydraulic systems. The correct operation of the entire hydraulic system often depends on its proper functioning. The wear and tear of individual pump components usually leads to a decrease in the pump’s operating pressure and volumetric losses, subsequently resulting in a decrease in overall pump efficiency and increases in vibration and pump noise. This in turn leads to an increase in energy losses throughout the hydraulic system, which releases excess heat. Typical failures of the discussed pumps and their causes are described after reviewing current research work using deep machine learning. Next, the test bench on which the diagnostic experiment was conducted and the selected operating signals that were recorded are described. The measured signals were subjected to a time–frequency analysis, and their features, calculated in terms of the time and frequency domains, underwent a significance ranking using the minimum redundancy maximum relevance (MRMR) algorithm. The next step was to design a neural network structure to classify the wear state of the pump and to test and evaluate the effectiveness of the network’s recognition of the pump’s condition. The whole study was summarized with conclusions.