bluuebunny/crossref_metadata_2025 huggingface dataset:
Effects of Therapeutic Horseback-Riding Program on Social and Communication Skills in Children with Autism Spectrum Disorder: A Systematic Review and Meta-Analysis
Authors: Chen Shihui , Zhang Yanjie , Zhao Mengxian , Du Xiru , Wang Yongtai , Liu Xiaolei
Published: November 2022

Animal-assisted therapy has become a fast-growing and effective approach for remediating core impairments of children with ASD; however, recent systematic review studies on the effects of AAT in children with ASD have some limitations, including referral to a variety of animal-assisted interventions rather than to horseback-riding therapy alone and the absence of any meta-analysis in systematic reviews. A complete systematic review of the studies that describe the use of THR as an intervention is needed to specifically target the core impairments of children with ASD. The purpose of this study was to employ the systematic review method to synthesize research findings regarding the effects of THR programs on the social interaction and communication skills of children with ASD. We conducted a structured search in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. We searched for potentially relevant studies in five databases (Web of Science, PubMed, CINAHL, Scopus, and SPORTDiscus) from inception until February 2022. In addition, we manually searched the bibliographies of the included studies to find articles that might otherwise have been missed. We considered articles eligible or ineligible if they satisfied specific inclusion or exclusion criteria. Our results showed that the THR program is an effective direct and alternative therapeutic program that can considerably improve the social behaviors and communication skills of children with ASD and can effectively impact autistic impairments in areas such as social awareness, social cognition, social motivation, and social communication. These findings are in line with those of previous studies; however, we did not find statistical evidence of any effect of THR on the autistic behaviors of irritability, stereotypy, and inappropriate speech. In conclusion, the findings produced by this meta-analysis study provide evidence that THR programs can considerably improve the social behaviors and communication skills of children with ASD.
Efficacy of a Contextualized Measurement of Life Satisfaction: A Pilot Study on the Assessment of Progress in Eating Disorder Therapy
Authors: Aymerich Maria , Castelló Antoni , Cladellas Ramon
Published: November 2022

Eating disorders strongly affect psychological distress and its perception. However, most of the existing instruments for assessing life satisfaction rely on a point-estimation method that is biased due to the circumstantial conditions around the time of assessment. The main goal of this study was to apply a different kind of instrument—the Life Satisfaction Chart—that situates the current state of life satisfaction in the context of personal history and describes the life stages through a graph. The assessment was applied to a sample of 29 adolescent women (average age of 17.88) who were enrolled in a clinical program to treat their eating disorders. The results showed that their estimation of their current life satisfaction was almost identical to the estimation provided by a therapist for those who were in therapy phases 1, 2, and 3 (of four), while patients’ point-estimation satisfaction showed statistically significant differences when compared with the situated estimations. In therapy phase 4, significant discrepancies were observed between the therapist’s perception and the patients’ perception, because the therapist focused only on eating disorder recovery, whilst the patients evaluated their lives under almost-normal conditions, taking into account further dimensions. The Life Satisfaction Chart is a new approach to life-satisfaction measurement that showed promising measurement and therapeutical properties.
How to Increase Sport Facility Users’ Intention to Use AI Fitness Services: Based on the Technology Adoption Model
Authors: Chin Ji-Hyoung , Do Chanwook , Kim Minjung
Published: November 2022

Artificial intelligence (AI) has recently been introduced as a new way of analyzing and predicting sport consumer behavior. The goal of this study was to investigate the relationships among the perceived usefulness, perceived ease of use, the importance of exercise, attitudes towards use, and the behavioral intention to use AI services based on the technology adoption model. The authors recruited 408 participants who participated in an experiment designed to provide a deeper understanding of AI fitness services. After screening, the collected data were screened through assumption tests, and we conducted a confirmatory factor analysis and structural equation modeling to analyze research hypotheses. The results indicated that three types of consumer evaluations (i.e., perceived usefulness, perceived ease of use, and importance of exercise) positively influence their attitudes toward AI fitness services. In addition, the positive attitudes regarding AI services positively influenced the intention to use AI services. The results of this research contribute to our knowledge of the consumers’ attitudes and behaviors toward AI services in the sport industry based on the technology acceptance model. Furthermore, this study provided the empirical evidence critically needed to increase our understanding of AI in the sport industry and offered new insights into how sport facility managers can predict their consumers’ intention to use AI services.
Temporal Trends in Acute Coronary Syndrome Mortality in Serbia in 2005–2019: An Age–Period–Cohort Analysis Using Data from the Serbian Acute Coronary Syndrome Registry (RAACS)
Authors: Vasić Ana , Vasiljević Zorana , Mickovski-Katalina Nataša , Mandić-Rajčević Stefan , Soldatović Ivan
Published: November 2022

Background: Cardiovascular diseases ranked first in terms of the number of deaths in Serbia in 2019, with 52,663 deaths. One fifth of those were from ischemic heart disease (IHD), and half of IHD deaths were from acute coronary syndrome (ACS). We present the ACS mortality time trend in Serbia during a 15-year period using the latest available data, excluding the COVID-19 pandemic. Methods: The data on patients who died of ACS in the period from 2005 to 2019 were obtained from the National Statistics Office and processed at the Department of Prevention and Control of Non-communicable Diseases of the Institute of Public Health of Serbia. Number of deaths, crude mortality rates (CR) and age-standardized mortality rates (ASR-E) for the European population were analyzed. Using joinpoint analysis, the time trend in terms of annual percentage change (APC) was analyzed for the female and male population aged 0 to 85+. Age–period–cohort modeling was used to estimate age, cohort and period effects in ACS mortality between 2005 and 2019 for age groups in the range 20 to 90. Results: From 2005 to 2019 there were 90,572 deaths from ACS: 54,202 in men (59.8%), 36,370 in women (40.2%). Over the last 15 years, the number of deaths significantly declined: 46.7% in men, 49.5% in women. The annual percentage change was significant: −4.4% in men, −5.8% in women. Expressed in terms of APC, for the full period, the highest significant decrease in deaths was seen in women aged 65–69, −8.5%, followed by −7.6% for women aged 50–54 and 70–74. In men, the highest decreases were recorded in the age group 50–54, −6.7%, and the age group 55–59, −5.7%. In all districts there was significant decline in deaths in terms of APC for the full period in both genders, except in Zlatibor, Kolubara and Morava, where increases were recorded. In addition, in Bor and Toplica almost no change was observed over the full period for both genders. Conclusions: In the last 15 years, mortality from ACS in Serbia declined in both genders. The reasons are found in better diagnostic and treatment through an organized network for management of ACS patients. However, there are districts where this decline was small and insignificant or was offset in recent years by an increase in deaths. In addition, there is space for improvement in the still-high mortality rates through primary prevention, which at the moment is not organized.
Response of Antibiotic Resistance Genes and Related Microorganisms to Arsenic during Vermicomposting of Cow Dung
Authors: Li Zijun , Chen Chen , Zhang Keqiang , Zhang Zulin , Zhao Ran , Han Bingjun , Yang Fengxia , Ding Yongzhen
Published: November 2022

Antibiotic resistance pollution in livestock manure is a persistent issue that has drawn public attention. Vermicomposting is an ecofriendly biological process that can render livestock manure harmless and resourceful. However, little is known about the impact of vermicomposting on antibiotic resistance in livestock manure under stress caused by potentially toxic arsenic levels. Herein, lab-scale vermicomposting was performed to comprehensively evaluate the shift in antibiotic resistance genes (ARGs) and related microorganisms in fresh earthworm casts as well as vermicompost product health (i.e., nutrient availability and enzyme activity) when they were fed on arsenic-contaminated cow manure. The results showed that the earthworms’ interaction with cow dung led to a significant reduction in ARG concentrations, especially for tetracycline ARGs (tet-ARGs), β-lactam ARGs (bla-ARGs), and quinolone ARGs (qnr-ARGs). However, arsenic significantly enhanced ARG accumulation in earthworm casts in a dose-dependent manner. Moreover, vermicomposting increased the percentage of Bacteroidota in the converted products. Furthermore, arsenic exposure at low concentrations promoted the proliferation of Proteobacteria, whereas high concentrations had little effect on Proteobacteria. Our study provides valuable insight into the changes in the antibiotic resistome and related microorganisms during vermicomposting of arsenic-amended cow manure, and it is crucial to explain the environmental impact of earthworms and improve our understanding of the reciprocal benefits of soil invertebrates.
Leukocyte Telomere Length as Potential Biomarker of HD Progression: A Follow-Up Study
Authors: Scarabino Daniela , Veneziano Liana , Mantuano Elide , Arisi Ivan , Fiore Alessia , Frontali Marina , Corbo Rosa Maria
Published: November 2022

The identification of biomarkers for neurodegenerative disorders such as Huntington’s disease (HD) is crucial for monitoring disease progression and therapeutic trial outcomes, especially in the pre-manifest disease stage (pre-HD). In a previous study, we observed that leukocyte telomere length (LTL) was strongly correlated with the estimated time to clinical onset in pre-HD subjects. To validate this hypothesis, we designed a follow-up study in which we analyzed LTL in 45 pre-HD stage subjects at baseline (T0) and then again after clinical onset at follow-up (T1); the follow-up interval was about 3 years, and the CAG range was 39–51 repeats; 90 peripheral blood mononuclear cell samples (PBMCs) were obtained from the Enroll-HD biorepository. In pre-HD subjects at T0, LTL was significantly reduced by 22% compared to the controls and by 14% from T0 at T1. No relationship was observed between the LTL and CAG numbers in subjects carrying different CAG repeats at T0 and at T1, suggesting that LTL reduction occurs independently of CAG number in pre-HD subjects. ROC curve analysis was used to test the validity of LTL as a potential biomarker of HD progression and showed that LTL measurement is extremely accurate in discriminating pre-HD subjects from the controls and even pre-HD from manifest HD, thus yielding a robust prognostic value in pre-HD subjects.
Microbiota Alterations in Patients with Autoimmune Thyroid Diseases: A Systematic Review
Authors: Sawicka-Gutaj Nadia , Gruszczyński Dawid , Zawalna Natalia , Nijakowski Kacper , Muller Ilaria , Karpiński Tomasz , Salvi Mario , Ruchała Marek
Published: November 2022

Autoimmune thyroid diseases (AITDs) are chronic autoimmune disorders that cause impaired immunoregulation, leading to specific immune responses against thyroid antigens. Graves’ disease (GD) and Hashimoto’s thyroiditis (HT) are the major forms of AITDs. Increasing evidence suggests a possible role of microbiota alterations in the pathogenesis and progression of AITDs. This systematic review was designed to address the following question: “Is microbiota altered in patients with AITDs?” After screening the selected studies using the inclusion and exclusion criteria, 16 studies were included in this review (in accordance with PRISMA statement guidelines). A meta-analysis revealed that patients with HT showed significantly higher values of diversity indices (except for the Simpson index) and that patients with GD showed significant tendencies toward lower values of all assessed indices compared with healthy subjects. However, the latter demonstrated a higher relative abundance of Bacteroidetes and Actinobacteria at the phylum level and thus Prevotella and Bifidobacterium at the genus level, respectively. Thyroid peroxidase antibodies showed the most significant positive and negative correlations between bacterial levels and thyroid functional parameters. In conclusion, significant alterations in the diversity and composition of the intestinal microbiota were observed in both GD and HT patients.
Selection of Diagnostically Significant Regions of the SLC26A4 Gene Involved in Hearing Loss
Authors: Danilchenko Valeriia Yu. , Zytsar Marina V. , Maslova Ekaterina A. , Posukh Olga L.
Published: November 2022

Screening pathogenic variants in the SLC26A4 gene is an important part of molecular genetic testing for hearing loss (HL) since they are one of the common causes of hereditary HL in many populations. However, a large size of the SLC26A4 gene (20 coding exons) predetermines the difficulties of its complete mutational analysis, especially in large samples of patients. In addition, the regional or ethno-specific prevalence of SLC26A4 pathogenic variants has not yet been fully elucidated, except variants c.919-2A>G and c.2168A>G (p.His723Arg), which have been proven to be most common in Asian populations. We explored the distribution of currently known pathogenic and likely pathogenic (PLP) variants across the SLC26A4 gene sequence presented in the Deafness Variation Database for the selection of potential diagnostically important parts of this gene. As a result of this bioinformatic analysis, we found that molecular testing ten SLC26A4 exons (4, 6, 10, 11, 13–17 and 19) with flanking intronic regions can provide a diagnostic rate of 61.9% for all PLP variants in the SLC26A4 gene. The primary sequencing of these SLC26A4 regions may be applied as an initial effective diagnostic testing in samples of patients of unknown ethnicity or as a subsequent step after the targeted testing of already-known ethno- or region-specific pathogenic SLC26A4 variants.
Generation and Characterization of Native and Sialic Acid-Deficient IgE
Authors: McCraw Alex J. , Gardner Richard A. , Davies Anna M. , Spencer Daniel I. R. , Grandits Melanie , Wagner Gerd K. , McDonnell James M. , Karagiannis Sophia N. , Chenoweth Alicia , Crescioli Silvia
Published: November 2022

Efficient characterization of IgE antibodies and their glycan structures is required for understanding their function in allergy and in the emerging AllergoOncology field for antibody immunotherapy. We report the generation, glyco-profiling and functional analysis of native and sialic acid-deficient glyco-engineered human IgE. The antibodies produced from human embryonic kidney cells were purified via a human IgE class-specific affinity matrix and structural integrity was confirmed by SDS-PAGE and size-exclusion chromatography (SEC). Purified IgEs specific for the tumor-associated antigens Chondroitin Sulfate Proteoglycan 4 (CSPG4-IgE) and Human Epidermal Growth Factor Receptor 2 (HER2-IgE) were devoid of by-products such as free light chains. Using neuraminidase-A, we generated sialic acid-deficient CSPG4-IgE as example glyco-engineered antibody. Comparative glycan analyses of native and glyco-engineered IgEs by Hydrophilic interaction liquid chromatography (HILIC)-high performance liquid chromatography (HPLC) indicated loss of sialic acid terminal residues and differential glycan profiles. Native and glyco-engineered CSPG4-IgEs recognized Fc receptors on the surface of human FcεRI-expressing rat basophilic leukemia RBL-SX38 cells, and of CD23/FcεRII-expressing human RPMI-8866 B-lymphocytes and bound to CSPG4-expressing A2058 human melanoma cells, confirming Fab-mediated recognition. When cross-linked on the cell surface, both IgEs triggered RBL-SX38 degranulation. We demonstrate efficient generation and functional competence of recombinant native and sialic acid-deficient IgEs.
The Role of Oxidation Pattern and Water Content in the Spatial Arrangement and Dynamics of Oxidized Graphene-Based Aqueous Dispersions
Authors: Rissanou Anastassia , Karnis Ioannis , Krasanakis Fanourios , Chrissopoulou Kiriaki , Karatasos Konstantinos
Published: November 2022

In this work, we employ fully atomistic molecular dynamics simulations to elucidate the effects of the oxidation pattern and of the water content on the organization of graphene sheets in aqueous dispersions and on the dynamic properties of the different moieties at neutral pH conditions. Analysis of the results reveals the role of the oxidation motif (peripherally or fully oxidized flakes) in the tendency of the flakes to self-assemble and in the control of key structural characteristics, such as the interlayer distance between the sheets and the average size and the distribution of the formed aggregates. In certain cases, the results are compared to a pertinent experimental system, validating further the relevant computational models. Examination of the diffusional motion of the oxidized flakes shows that different degrees of spatial restriction are imposed upon the decrease in the water content and elucidates the conditions under which a motional arrest of the flakes takes place. At constant water content, the structural differences between the formed aggregates appear to additionally impart distinct diffusional characteristics of a water molecule. A detailed examination of the counterion dynamics describes their interaction with the oxidized flakes and their dependence on the water content and on the oxidation pattern, offering new insight into the expected electrical properties of the dispersions. The detailed information provided by this work will be particularly useful in applications such as molecular sieving, nanofiltration, and in cases where conductive membranes based on oxidized forms of graphene are used.
Quantitative Proteomics Analysis Reveals That Cyclooxygenase-2 Modulates Mitochondrial Respiratory Chain Complex IV in Cardiomyocytes
Authors: Alvarez Maria Soledad , Núñez Estefanía , Fuertes-Agudo Marina , Cucarella Carme , Fernandez-Velasco Maria , Boscá Lisardo , Vázquez Jesús , Rossignol Rodrigue , Martin-Sanz Paloma , Casado Marta
Published: November 2022

The biochemical mechanisms of cell injury and myocardial cell death after myocardial infarction remain unresolved. Cyclooxygenase 2 (COX-2), a key enzyme in prostanoid synthesis, is expressed in human ischemic myocardium and dilated cardiomyopathy, but it is absent in healthy hearts. To assess the role of COX-2 in cardiovascular physiopathology, we developed transgenic mice that constitutively express functional human COX-2 in cardiomyocytes under the control of the α-myosin heavy chain promoter. These animals had no apparent phenotype but were protected against ischemia-reperfusion injury in isolated hearts, with enhanced functional recovery and diminished cellular necrosis. To further explore the phenotype of this animal model, we carried out a differential proteome analysis of wild-type vs. transgenic cardiomyocytes. The results revealed a tissue-specific proteomic profile dominated by mitochondrial proteins. In particular, an increased expression of respiratory chain complex IV proteins was observed. This correlated with increased catalytic activity, enhanced respiratory capacity, and increased ATP levels in the heart of COX-2 transgenic mice. These data suggest a new link between COX-2 and mitochondria, which might contribute to the protective cardiac effects of COX-2 against ischemia-reperfusion injury.
Ibrutinib Prevents Acute Lung Injury via Multi-Targeting BTK, FLT3 and EGFR in Mice
Authors: Rao Huanan , Song Xiaominting , Lei Jieting , Lu Peng , Zhao Guiying , Kang Xin , Zhang Duanna , Zhang Tingrui , Ren Yali , Peng Cheng , Li Yuzhi , Pei Jin , Cao Zhixing
Published: November 2022

Ibrutinib has potential therapeutic or protective effects against viral- and bacterial-induced acute lung injury (ALI), likely by modulating the Bruton tyrosine kinase (BTK) signaling pathway. However, ibrutinib has multi-target effects. Moreover, immunity and inflammation targets in ALI treatment are poorly defined. We investigated whether the BTK-, FLT3-, and EGFR-related signaling pathways mediated the protective effects of ibrutinib on ALI. The intratracheal administration of poly I:C or LPS after ibrutinib administration in mice was performed by gavage. The pathological conditions of the lungs were assessed by micro-CT and HE staining. The levels of neutrophils, lymphocytes, and related inflammatory factors in the lungs were evaluated by ELISA, flow cytometry, immunohistochemistry, and immunofluorescence. Finally, the expression of proteins associated with the BTK-, FLT3-, and EGFR-related signaling pathways were evaluated by Western blotting. Ibrutinib (10 mg/kg) protected against poly I:C-induced (5 mg/kg) and LPS-induced (5 mg/kg) lung inflammation. The wet/dry weight ratio (W/D) and total proteins in the bronchoalveolar lavage fluid (BALF) were markedly reduced after ibrutinib (10 mg/kg) treatment, relative to the poly I:C- and LPS-treated groups. The levels of ALI indicators (NFκB, IL-1β, IL-6, TNF-α, IFN-γ, neutrophils, and lymphocytes) were significantly reduced after treatment. Accordingly, ibrutinib inhibited the poly I:C- and LPS-induced BTK-, FLT3-, and EGFR-related pathway activations. Ibrutinib inhibited poly I:C- and LPS-induced acute lung injury, and this may be due to its ability to suppress the BTK-, FLT3-, and EGFR-related signaling pathways. Therefore, ibrutinib is a potential protective agent for regulating immunity and inflammation in poly I:C- and LPS-induced ALI.
Chromium Induces Toxicity at Different Phenotypic, Physiological, Biochemical, and Ultrastructural Levels in Sweet Potato (Ipomoea batatas L.) Plants
Authors: Kumar Sunjeet , Wang Mengzhao , Fahad Shah , Qayyum Abdul , Chen Yanli , Zhu Guopeng
Published: November 2022

Crop productivity is enormously exposed to different environmental stresses, among which chromium (Cr) stress raises considerable concerns and causes a serious threat to plant growth. This study explored the toxic effect of Cr on sweet potato plants. Plants were hydroponically grown, and treatments of 0, 25, 50, 100, and 200 µM Cr were applied for seven days. This study exhibited that a low level of Cr treatment (25 µM) enhanced the growth, biomass, photosynthesis, osmolytes, antioxidants, and enzyme activities. However, significant deleterious effects in growth, biomass, photosynthetic attributes, antioxidants, and enzymes were observed at higher levels of Cr treatment. The remarkable reduction in plant growth traits was associated with the over-accumulation of H2O2 and MDA contents (410% and 577%, respectively) under the highest rate of Cr (200 µM). Under 200 µM Cr, the uptake in the roots were 27.4 mg kg−1 DW, while in shoots were 11 mg kg−1 DW with the highest translocation rate from root to shoot was 0.40. The results showed that the higher accumulation of Cr negatively correlated with the phenotypic and physiological parameters. It may be proposed that Cr toxicity causes oxidative damage as sustained by augmented lipid peroxidation, reactive oxygen species, and reduced photosynthetic rate, chlorophyll, and stomatal traits. The chloroplastic ultrastructure was damaged, and more apparent damage and size reduction were observed at higher Cr levels. Furthermore, aggregated Cr concentration positively correlates with the increase of osmolytes and superoxide dismutase (SOD) activity in the leaves of sweet potato. Moreover, improved osmolytes and SOD do not help protect sweet potato against high Cr stress. Overall, these findings will improve the understanding of the defense mechanisms of sweet potato to Cr stress.
Identification of Selective BRD9 Inhibitor via Integrated Computational Approach
Authors: Ali Maria Mushtaq , Ashraf Sajda , Nure-e-Alam Mohammad , Qureshi Urooj , Khan Khalid Mohammed , Ul-Haq Zaheer
Published: November 2022

Bromodomain-containing protein 9 (BRD9), a member of the bromodomain and extra terminal domain (BET) protein family, works as an epigenetic reader. BRD9 has been considered an essential drug target for cancer, inflammatory diseases, and metabolic disorders. Due to its high similarity among other isoforms, no effective treatment of BRD9-associated disorders is available. For the first time, we performed a detailed comparative analysis among BRD9, BRD7, and BRD4. The results indicate that residues His42, Gly43, Ala46, Ala54, Val105, and Leu109 can confer the BRD9 isoform selectivity. The predicted crucial residues were further studied. The pharmacophore model’s features were precisely mapped with some key residues including, Gly43, Phe44, Phe45, Asn100, and Tyr106, all of which play a crucial role in BRD9 inhibition. Docking-based virtual screening was utilized with the consideration of the conserved water network in the binding cavity to identify the potential inhibitors of BRD9. In this workflow, 714 compounds were shortlisted. To attain selectivity, 109 compounds were re-docked to BRD7 for negative selection. Finally, four compounds were selected for molecular dynamics studies. Our studies pave the way for the identification of new compounds and their role in causing noticeable, functional differences in isoforms and between orthologues.
Androgen Metabolism and Response in Prostate Cancer Anti-Androgen Therapy Resistance
Authors: Zhang Haozhe , Zhou Yi , Xing Zengzhen , Sah Rajiv Kumar , Hu Junqi , Hu Hailiang
Published: November 2022

All aspects of prostate cancer evolution are closely related to androgen levels and the status of the androgen receptor (AR). Almost all treatments target androgen metabolism pathways and AR, from castration-sensitive prostate cancer (CSPC) to castration-resistant prostate cancer (CRPC). Alterations in androgen metabolism and its response are one of the main reasons for prostate cancer drug resistance. In this review, we will introduce androgen metabolism, including how the androgen was synthesized, consumed, and responded to in healthy people and prostate cancer patients, and discuss how these alterations in androgen metabolism contribute to the resistance to anti-androgen therapy.
Postbiotics Prepared Using Lactobacillus paracasei CCFM1224 Prevent Nonalcoholic Fatty Liver Disease by Modulating the Gut Microbiota and Liver Metabolism
Authors: Pan Zhenghao , Mao Bingyong , Zhang Qiuxiang , Tang Xin , Yang Bo , Zhao Jianxin , Cui Shumao , Zhang Hao
Published: November 2022

Postbiotics are rich in a variety of bioactive components, which may have beneficial effects in inhibiting hepatic lipid accumulation. In this study, we investigated the preventive effects of postbiotics (POST) prepared from Lactobacillus paracasei on non-alcoholic fatty liver disease (NAFLD). Our results showed that when mice ingested a high-fat diet (HFD) and POST simultaneously, weight gain was slowed, epididymal white fat hypertrophy and insulin resistance were suppressed, serum biochemical indicators related to blood lipid metabolism were improved, and hepatic steatosis and liver inflammation decreased. Bacterial sequencing showed that POST modulated the gut microbiota in HFD mice, increasing the relative abundance of Akkermansia and reducing the relative abundance of Lachnospiraceae NK4A136 group, Ruminiclostridium and Bilophila. Spearman’s correlation analysis revealed significant correlations between lipid metabolism parameters and gut microbes. Functional prediction results showed that the regulation of gut microbiota was associated with the improvement of metabolic status. The metabolomic analysis of the liver revealed that POST-regulated liver metabolic pathways, such as glycerophospholipid and ether lipid metabolism, pantothenate and CoA biosynthesis, some parts of amino acid metabolism, and other metabolic pathways. In addition, POST regulated the gene expression in hepatocytes at the mRNA level, thereby regulating lipid metabolism. These findings suggest that POST plays a protective role against NAFLD and may exert its efficacy by modulating the gut microbiota and liver metabolism, and these findings may be applied to related functional foods.
Behavior of Pultruded Glass-Fiber-Reinforced Polymer Beam-Columns Infilled with Engineered Cementitious Composites under Cyclic Loading
Authors: Chinnasamy Yoganantham , Joanna Philip Saratha , Kothanda Karthikeyan , Gurupatham Beulah Gnana Ananthi , Roy Krishanu
Published: November 2022

Glass-fiber-reinforced polymer (GFRP) is an advanced material that has superior corrosion resistance, a high strength-to-weight ratio, low thermal conductivity, high stiffness, high fatigue strength, and the ability to resist chemical and microbiological compounds. Despite their many advantages compared with traditional materials, GFRP sections exhibit brittle behavior when subjected to severe loading conditions such as earthquakes, which could be overcome by infilling the GFRP sections with concrete. This paper presents the results of an experimental investigation carried out on the cyclic response of a GFRP beam-column infilled with high-volume fly ash engineered cementitious composites (HVFA-ECC) consisting of 60%, 70%, and 80% fly ash as a replacement for cement. Finite element analysis was also conducted using robot structural analysis software, and the results were compared with the experimental results. The mechanical properties of GFRP sections presented are the compressive strength of ECC, the direct tensile strength of ECC determined using a dog-bone-shaped ECC specimen, the hysteresis behavior of the beam-column, and the energy dissipation characteristics. The lateral load-carrying capacity of beam-column GFRP infilled with HVFA-ECC consisting of 60%, 70%, and 80% fly ash was found to be, respectively, 43%, 31%, and 20% higher than the capacity of GFRP beam-columns without any infill. Hence the GFRP sections infilled with HVFA-ECC could be used as lightweight structural components in buildings to be constructed in earthquake-prone areas. Also in the structural components, as 70% of cement could be replaced with fly ash, it can potentially lead to sustainable construction.
Adhesion to a CAD/CAM Composite: Causal Factors for a Reliable Long-Term Bond
Authors: Duma Sandra , Ilie Nicoleta
Published: November 2022

Computer aided design/manufacturing (CAD/CAM) technology has become an increasingly popular part of dentistry, which today also includes CAD/CAM resin-based composite (RBC) applications. Because CAD/CAM RBCs are much more difficult to bond, many methods and attachment materials are still being proposed, while the best application method is still a matter of debate. The present study therefore evaluates causal factors for a reliable long-term bond, which includes the surface preparation of the CAD/CAM RBC, aging and the type of luting material. The reliability of the bond was calculated, and supplemented by fractography to identify fracture mechanisms. Five categories of luting materials were used: (1) temporary zinc phosphate cement, (2) glass ionomer cement (GIC), (3) resin-modified GIC, (4) conventional adhesive resin cement (ARC), and (5) self-adhesive RC. Half of the CAD/CAM RBC surfaces (n = 200) were sandblasted (SB) with 50 µm aluminum oxide, while the other half remained untreated. Bond strength measurements of the 400 resulting specimens were carried out after 24 h (n = 200) or after additional aging (10,000 thermo-cycles between 5 and 55 °C) (n = 200). The data were statistically analyzed using one- and three-way ANOVA followed by Games-Howell post-hoc test (α = 0.05) and Weibull analysis. Aging resulted in a significant decrease in bond strength primarily for the conventional cements. The highest bond strengths and reliabilities were recorded for both ARCs. SB caused a significant increase in bond strength for most luting materials, but also caused microcracks in the CAD/CAM RBC. These microcracks might compromise the long-term reliability of the bond in vivo.
Molecular Technology for Isolation and Characterization of Mitogen-Activated Protein Kinase Kinase 4 from Penaeus monodon, and the Response to Bacterial Infection and Low-Salinity Challenge
Authors: Li Yundong , Zhou Falin , Fan Hongdi , Jiang Song , Yang Qibin , Huang Jianhua , Yang Lishi , Chen Xu , Zhang Wenwen , Jiang Shigui
Published: November 2022

Mitogen-activated protein kinase kinase 4 (MKK4) is a component of the JNK signaling pathway and plays an important role in immunity and stress resistance. In this study, MKK4 cDNA was cloned, and its bacterial infection and low-salinity challenge responses were researched. The full-length PmMKK4 cDNA was 1582 bp long, with an 858-bp open reading frame (ORF) encoding a 285-amino acid (aa) protein. Results showed that PmMKK-4 was expressed in all examined tissues of P. monodon. The PmMKK4 expression level was found to be lowest in eyestalk ganglion and highest in muscle (approximately 41.25 times than in eyestalk ganglion). Following the infection of Staphylococcus aureus, PmMKK4 was up-regulated in both hepatopancreatic and gill tissues. However, after infection with Vibrio harveyi, PmMKK4 was down-regulated for a period of time in gill tissue, with fluctuating up- and down-regulation in hepatopancreas tissue. Furthermore, after infection with Vibrio anguillarum, gill tissue and hepatopancreas tissue showed a continuous downward trend. The PmMKK4 gene in the gill tissue and hepatopancreas tissue of P. monodon was activated after low-salinity stress. The expression change of PmMKK4 in gill tissue was more significant. The research showed that the PmMKK4 gene plays an important role in both innate immunities after pathogen infection and adaptation in a low-salt environment.
A Multi-Criteria Approach for Evaluating a Sustainable Intermodal Transport Chain Affected by the COVID-19 Pandemic
Authors: Golnar Marko , Beškovnik Bojan
Published: November 2022

The sustainable performance of the intermodal transport chain has gained popularity in recent decades, especially due to climate change and numerous European laws aimed at minimizing the negative impacts of transport. In this paper, we have developed a novel three-phase, two-stage approach that is a combination of distance-based analytic hierarchy process/data envelopment analysis (AHP-DEA). The added value of this multi-criteria approach is in evaluating a sustainable intermodal transport chain, with prioritization of the most efficient combinations of transport in accordance with the weights derived from its users. Instead of the classic pairwise comparison, the weights of the criteria were determined using a new distance-based AHP method in which respondents were asked to sort the criteria (transportation time, price, emissions, and variability) pre-selected from the literature in order of greatest importance. Therefore, the approach determines the most efficient transport chain in the transportation corridor. Since a transportation corridor was previously defined, the settings for this corridor were set to constant initial variables. In this way, the above criteria were chosen as inputs, with DEA aimed at minimizing these variables and presenting the results in ranks from highest to lowest efficiency. The potential of our approach was presented in a case study, where the most efficient of the selected transport chains between Asia and the northern Adriatic were chosen. The results show that there are different intermodal transport chains, each of which consists of either maritime and rail transport or maritime and road transport. The paper concludes that the presented multicriteria approach has greater discriminatory power than the current DEA, as well as greater flexibility, since the weights can be derived faster and more effortlessly than is typical. Therefore, this method can help transportation organizers to determine which intermodal transportation chain is the most efficient or sustainable in any given situation.
Game Change: A Critical Review of Applicable Collision Avoidance Rules between Traditional and Autonomous Ships
Authors: Zhang Pengfei , Chen Qiong , Macdonald Tobias , Lau Yui-Yip , Tang Yuk-Ming
Published: November 2022

Since the rise of intelligent control and multi-sensor integration technology, the development of autonomous ships has been significantly growing over the last decade. This advancement has painted a picture of extreme change with a radical alteration of human factors and new operating models. Inherent with the development of such ships, some concerns regarding their safe operation and integration into the maritime regulatory framework arose. Although the introduction of autonomous vessels is not an impending factor, it is the future, and one day will come into application. The primary concern inherent in the development of autonomous ships is compliance with the current International Regulations for Preventing Collisions at Sea (COLREGS) 1972. This paper uses an interdisciplinary approach to examine autonomous vessel seaborne interactions. The results show that we should actively support the modernization of the maritime industry and integrate it with other autonomous industries in the world.
Readiness to Accept Genetic Testing for Personalized Medicine: Survey Findings on the Role of Socio-Demographic Characteristics, Health Vulnerabilities, Perceived Genetic Risk and Personality Factors
Authors: Leppin Anja , Nielsen Jesper Bo
Published: November 2022

Studies from various countries have shown that majorities would accept genetic testing for personalization of treatment, but little is known about differences among population subgroups. The present study investigated whether readiness to accept a hypothetical cost-free offer of genetic testing to personalize treatment depends on socio-demographic characteristics, health-related vulnerabilities, personal dispositions, and prior awareness about personalized medicine. The study was based on a cross-sectional survey design. Out of a representative initial sample of 50–80-year-old Danish citizens (n = 15,072), n = 6807 returned a fully answered web-based questionnaire. Socio-demographic data were added from a national registry. Data were analyzed by multivariable logistic regression. A large majority of respondents (78.3%) expressed their readiness to be tested. Rates were higher in men, younger persons, and those with higher income. Additionally, ex-smokers and obese persons as well as those less satisfied with their health and respondents who perceived a personal genetic risk were more interested, as were those with higher internal health control, higher extraversion, higher emotional stability, and those who had not heard about this option before. Further research should investigate the specific concerns among population subgroups which need being addressed by systematic communication efforts in a clinical but also a broader public health context.
What Can Aphasia Tell Us about How the First-Acquired Language Is Instantiated in the Brain?
Authors: Goral Mira
Published: November 2022

Recent neurolinguistic theories converge on the hypothesis that the languages of multilingual people are processed as one system in the brain. One system for the multiple languages is also at the core of a translanguaging framework of multilingualism—a framework that focuses on each speaker’s complete linguistic repertoire rather than on the separate languages they know. However, evidence from neuroimaging studies suggests at least some nonoverlapping activations of the first-acquired language (L1) and other (non-L1) languages of multilingual people, especially when the age of acquisition and/or levels of proficiency differ across the languages. Neurolinguistic studies of acquired language disorders have demonstrated that in multilingual people who experience language impairments due to brain lesion, L1 may be less impaired or better recovered than non-L1. This paper explores the evidence available to date from the study of acquired language impairment regarding this potential primacy of the first-acquired language. Findings suggest that L1 may be better preserved in many instances of language impairment, challenging the theory of a single system for multiple languages.
Interlaminar Microstructure and Mechanical Properties of Narrow Gap Laser Welding of 40-mm-Thick Ti-6Al-4V Alloy
Authors: Liu Xing , Ling Wanli , Li Yue , Wang Jianfeng , Zhan Xiaohong
Published: November 2022

Narrow gap laser welding (NGLW) is a common solution for the welding of thick structures. NGLW was carried out on narrow-gap butt joints of 40 mm-thick Ti-6Al-4V alloy plates with a U-shaped groove. The distribution characteristics of the interlaminar microstructure in different height ranges of the joint were investigated, and the evolution behavior and formation mechanism of the interlaminar microstructure of the joint were also revealed. This showed that a large amount of short needle martensite nucleated and grew up near the fusion line and the upper boundary of the remelting zone. The “softening” phenomenon occurred in all welds except the cover layer weld. The microstructure evolution and defect migration, induced by multiple welding thermal cycles in the upper weld forming process, were the main reasons for the “softening” of the lower weld. The tensile strength of each sample changed in the range of 920~990 MPa; the fracture mode of the sample belongs to a transgranular ductile fracture. In addition, compared with the upper part of the joint, the plasticity and toughness of the weld area in the lower part of the joint was improved.
Electrochemical Machining of Curvilinear Surfaces of Revolution: Analysis, Modelling, and Process Control
Authors: Sawicki Jerzy , Paczkowski Tomasz
Published: November 2022

The paper presents the authors’ model for the adaptive control of the electrochemical machining (ECM) process of machining the rotary (axisymmetric) elements of any curvilinear shape, using the results of theoretical computer simulation of this process. Computer simulations have been based on the authors’ model of the ECM of rotary surfaces of any curvilinear shape. The quasi- 3D ECM model proposed facilitates an analysis of physical phenomena which occur in the interelectrode gap. Mathematical ECM modelling has been based on the application of the equation of the workpiece shape evolution and on the system of partial differential equations resulting from the principle of mass conservation, momentum and the law of conservation of energy describing a flow of the mixture of electrolyte in the interelectrode gap. A solution to the problem has been developed with analytical and numerical integration. For the rotary hemispheric surface, in a set time, the local machining of a change in the interelectrode gap thickness and characteristic physicochemical parameters were determined, especially static pressure distribution, electrolyte flow velocity, temperature and volumetric gas phase concentration as well as current density. The simulation results were experimentally verified by determining the distribution of the shape deviation (WP) calculated from the process computer simulation and after the ECM. Applying the adaptive control of the ECM process has facilitated, based on the simulations made, enhancing the process stability and avoiding the occurrence of critical states.
Effects of Elastic Couplings in a Compressed Plate Element with Cut-Out
Authors: Falkowicz Katarzyna , Samborski Sylwester , Valvo Paolo Sebastiano
Published: November 2022

Analytical calculations were performed on carbon fiber-reinforced polymer (CFRP) laminates in an asymmetrical configuration. The asymmetric configuration of composites was investigated, where extension–twisting and extension–bending couplings were used to obtain the elastic element. Analysis of the presence of elastic couplings was conducted according to Classical Laminate Theory (CLT). Components of matrices A, B, and D, as well as the parameters Dc and Bt, were obtained using the MATLAB software environment. The results show that couplings between the extension and bending, as well as between the extension and twisting, were strongly dependent on specimen plies’ orientation. Moreover, additional analysis was performed on the influence of layer angle on the terms which are components of the Bt and Dc coefficients. The results indicate that the angle of laying fibers around 45–50° significantly amplifies the effects of elastic couplings.
Influence of Graphene Type and Content on Friction and Wear of Silicon Carbide/Graphene Nanocomposites in Aqueous Environment
Authors: Schlüter Bernadette , Schröder Christian , Zhang Wenli , Mülhaupt Rolf , Degenhardt Ulrich , Sedlák Richard , Dusza Ján , Balázsi Katalin , Balázsi Csaba , Kailer Andreas
Published: November 2022

Two different types of graphene materials were used as functional nanofillers for the mechanical and tribological improvement of silicon carbide/graphene nanocomposites. On the one hand is thermally reduced graphite oxide (TRGO) reduced at three different temperatures, and on the other hand is graphene made of three different organic precursors, which were directly coated on silicon carbide (SiC) platelets (GSiC). Additionally, benchmark materials were also used as carbon fillers. The SiC/graphene nanocomposites with 2 wt% filler content were manufactured by pressureless sintering (PLS). Some composites were produced with higher graphene contents of 4% and 8% and sintered by spark plasma sintering (SPS). Microstructural analyses were conducted using scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Underwater lubrication, the SP sintered TRGO and GSiC materials with high graphene content have shown the most promising tribological performance. Furthermore, the reduced size of the homogeneously distributed nanoparticles promotes the formation of surface states, which improve the friction and wear properties.
Influences of the Contact State between Friction Pairs on the Thermodynamic Characteristics of a Multi-Disc Clutch
Authors: Yu Liang , Zheng Changsong , Wang Liyong , Wu Jianpeng , Jia Ran
Published: November 2022

The relationship between clutch thermodynamic characteristics and contact states of friction components is explored numerically and experimentally. The clutch thermodynamic numerical model is developed with consideration of the contact state and oil film between friction pairs. The clutch bench test is conducted to verify the variation of the clutch thermodynamic characteristics from the uniform contact (UCS) to the intermittent contact (ICS). The results show that the oil film decreases gradually with increasing temperature; the lubrication state finally changes from hydrodynamic lubrication to dry friction, where the friction coefficient shows an increasing trend before a decrease. Thus, the friction torque in UCS gradually increases after the applied pressure stabilizes. When the contact state changes to ICS, the contact pressure increases suddenly and the oil film decreases rapidly in the local contact area, bringing about a sharp increase in friction torque; subsequently, the circumferential and radial temperature differences of friction components expand dramatically. However, if the contact zone is already in the dry friction state, friction torque declines directly, resulting in clutch failure. The conclusions can potentially be used for online monitoring and fault diagnosis of the clutch.