bluuebunny/crossref_metadata_2025 huggingface dataset:
Phenotypic and transcriptional characterization of F. tularensis LVS during transition into a viable but non-culturable state
Authors: Cantlay Stuart , Garrison Nicole L. , Patterson Rachelle , Wagner Kassey , Kirk Zoei , Fan Jun , Primerano Donald A. , Sullivan Mara L. G. , Franks Jonathan M. , Stolz Donna B. , Horzempa Joseph
Published: February 2024

Francisella tularensis is a gram-negative, intracellular pathogen which can cause serious, potentially fatal, illness in humans. Species of F. tularensis are found across the Northern Hemisphere and can infect a broad range of host species, including humans. Factors affecting the persistence of F. tularensis in the environment and its epidemiology are not well understood, however, the ability of F. tularensis to enter a viable but non-culturable state (VBNC) may be important. A broad range of bacteria, including many pathogens, have been observed to enter the VBNC state in response to stressful environmental conditions, such as nutrient limitation, osmotic or oxidative stress or low temperature. To investigate the transition into the VBNC state for F. tularensis, we analyzed the attenuated live vaccine strain, F. tularensis LVS grown under standard laboratory conditions. We found that F. tularensis LVS rapidly and spontaneously enters a VBNC state in broth culture at 37°C and that this transition coincides with morphological differentiation of the cells. The VBNC bacteria retained an ability to interact with both murine macrophages and human erythrocytes in in vitro assays and were insensitive to treatment with gentamicin. Finally, we present the first transcriptomic analysis of VBNC F. tularensis, which revealed clear differences in gene expression, and we identify sets of differentially regulated genes which are specific to the VBNC state. Identification of these VBNC specific genes will pave the way for future research aimed at dissecting the molecular mechanisms driving entry into the VBNC state.
Combination of nucleic acid amplification and CRISPR/Cas technology in pathogen detection
Authors: Zeng Dandan , Jiao Jinlong , Mo Tianlu
Published: February 2024

Major health events caused by pathogenic microorganisms are increasing, seriously jeopardizing human lives. Currently PCR and ITA are widely used for rapid testing in food, medicine, industry and agriculture. However, due to the non-specificity of the amplification process, researchers have proposed the combination of nucleic acid amplification technology with the novel technology CRISPR for detection, which improves the specificity and credibility of results. This paper summarizes the research progress of nucleic acid amplification technology in conjunction with CRISPR/Cas technology for the detection of pathogens, which provides a reference and theoretical basis for the subsequent application of nucleic acid amplification technology in the field of pathogen detection.
Motor imagery ability in children and adolescents with cerebral palsy: a systematic review and evidence map
Authors: Fierro-Marrero José , Corujo-Merino Alejandro , La Touche Roy , Lerma-Lara Sergio
Published: February 2024

Background

Cerebral palsy (CP) refers to a group of permanent movement and posture disorders. Motor imagery (MI) therapy is known to provide potential benefits, but data on MI ability in children and adolescents with CP is lacking.

Objective

A systematic review was performed to explore MI abilities in children and adolescents with CP compared to typically developed (TD) subjects.

Methods

We searched on PubMed, Web of Science (WOS), EBSCO, Google Scholar, and PEDro including observational studies. Methodological quality was assessed with the modified Newcastle–Ottawa Scale and evidence map was created to synthesize the evidence qualitatively and quantitatively.

Results

Seven cross-sectional studies were selected, which included 174 patients with CP and 321 TD subjects. Three studies explored explicit MI, two MI–execution synchrony, and four implicit MI domains. Methodological quality ranged from 6 to 8 stars. Moderate evidence supported the absence of differences in vividness between the groups. As there was only limited evidence, establishing a clear direction for the results was not possible, especially for the capacity to generate MI, mental chronometry features, and MI–execution synchrony domains. Moderate evidence supported a lower efficiency in cases for hand recognition, derived from a lower accuracy rate, while reaction time remained similar between the two groups. Moderate evidence indicated that patients with CP and TD controls showed similar features on whole-body recognition.

Conclusion

Moderate evidence suggests that patients with CP present a reduced ability in hand recognition, which is not observed for whole-body recognition compared to healthy controls. Severe limitations concerning sample size calculations and validity of assessment tools clearly limits establishing a direction of results, especially for explicit MI and MI-Execution synchrony domains. Further research is needed to address these limitations to enhance our comprehension of MI abilities in children, which is crucial for prescribing suitable MI-based therapies in this child population.
A missense mutation in the Hspa8 gene encoding heat shock cognate protein 70 causes neuroaxonal dystrophy in rats
Authors: Tanaka Miyuu , Fujikawa Ryoko , Sekiguchi Takahiro , Hernandez Jason , Johnson Oleta T. , Tanaka Daisuke , Kumafuji Kenta , Serikawa Tadao , Hoang Trung Hieu , Hattori Kosuke , Mashimo Tomoji , Kuwamura Mitsuru , Gestwicki Jason E. , Kuramoto Takashi
Published: February 2024

Neuroaxonal dystrophy (NAD) is a neurodegenerative disease characterized by spheroid (swollen axon) formation in the nervous system. In the present study, we focused on a newly established autosomal recessive mutant strain of F344-kk/kk rats with hind limb gait abnormalities and ataxia from a young age. Histopathologically, a number of axonal spheroids were observed throughout the central nervous system, including the spinal cord (mainly in the dorsal cord), brain stem, and cerebellum in F344-kk/kk rats. Transmission electron microscopic observation of the spinal cord revealed accumulation of electron-dense bodies, degenerated abnormal mitochondria, as well as membranous or tubular structures in the axonal spheroids. Based on these neuropathological findings, F344-kk/kk rats were diagnosed with NAD. By a positional cloning approach, we identified a missense mutation (V95E) in the Hspa8 (heat shock protein family A (Hsp70) member 8) gene located on chromosome 8 of the F344-kk/kk rat genome. Furthermore, we developed the Hspa8 knock-in (KI) rats with the V95E mutation using the CRISPR-Cas system. Homozygous Hspa8-KI rats exhibited ataxia and axonal spheroids similar to those of F344-kk/kk rats. The V95E mutant HSC70 protein exhibited the significant but modest decrease in the maximum hydrolysis rate of ATPase when stimulated by co-chaperons DnaJB4 and BAG1 in vitro, which suggests the functional deficit in the V95E HSC70. Together, our findings provide the first evidence that the genetic alteration of the Hspa8 gene caused NAD in mammals.
Variational mode decomposition-based EEG analysis for the classification of disorders of consciousness
Authors: Raveendran Sreelakshmi , Kenchaiah Raghavendra , Kumar Santhos , Sahoo Jayakrushna , Farsana M. K. , Chowdary Mundlamuri Ravindranadh , Bansal Sonia , Binu V. S. , Ramakrishnan A. G. , Ramakrishnan Subasree , Kala S.
Published: February 2024

Aberrant alterations in any of the two dimensions of consciousness, namely awareness and arousal, can lead to the emergence of disorders of consciousness (DOC). The development of DOC may arise from more severe or targeted lesions in the brain, resulting in widespread functional abnormalities. However, when it comes to classifying patients with disorders of consciousness, particularly utilizing resting-state electroencephalogram (EEG) signals through machine learning methods, several challenges surface. The non-stationarity and intricacy of EEG data present obstacles in understanding neuronal activities and achieving precise classification. To address these challenges, this study proposes variational mode decomposition (VMD) of EEG before feature extraction along with machine learning models. By decomposing preprocessed EEG signals into specified modes using VMD, features such as sample entropy, spectral entropy, kurtosis, and skewness are extracted across these modes. The study compares the performance of the features extracted from VMD-based approach with the frequency band-based approach and also the approach with features extracted from raw-EEG. The classification process involves binary classification between unresponsive wakefulness syndrome (UWS) and the minimally conscious state (MCS), as well as multi-class classification (coma vs. UWS vs. MCS). Kruskal-Wallis test was applied to determine the statistical significance of the features and features with a significance of p < 0.05 were chosen for a second round of classification experiments. Results indicate that the VMD-based features outperform the features of other two approaches, with the ensemble bagged tree (EBT) achieving the highest accuracy of 80.5% for multi-class classification (the best in the literature) and 86.7% for binary classification. This approach underscores the potential of integrating advanced signal processing techniques and machine learning in improving the classification of patients with disorders of consciousness, thereby enhancing patient care and facilitating informed treatment decision-making.
Exploring gene signatures and regulatory networks in a rat model of sciatica: implications and validation in neuropathic pain
Authors: Xu Mu , Wang Zhijian , Xu Gang , Zhu Mengye , Zhang Daying , Yan Yi
Published: February 2024

Background

Sciatica (neuropathic pain [NP]) is a common disease characterized by pain from radiation along the sciatic nerve. The aim of this study was to study the genes associated with chronic systolic injury of sciatic nerve (SCN-CCI) in rats by RNA-Seq technique, and to explore their potential as therapeutic targets.

Methods

Sciatic nerve rat model was obtained by ligation of sciatic nerve and divided into two groups: SCN-CCI group and Sham group. Behavioral assessments were performed to evaluate pain sensitivity, following which their spinal cord dorsal horn were resected and RNA sequencing was conducted to identify differentially expressed genes (DEGs). Bioinformatics and functional enrichment analysis was performed to identify promising DEGs and their related biological processes and pathways associated with SCN-CCI. PPI network analysis and hub gene identification were conducted. QRT-PCR, western blot, ELISA, and immunofluorescence staining were performed on rat models to validate the expression of these hub genes and investigate related proteins and inflammatory markers.

Results

The SCN-CCI rat model was successfully obtained, exhibiting increased pain sensitivity compared to the Sham group, as indicated by decreased mechanical allodynia thresholds, thermal latencies, and increased paw withdrawals. RNA-Seq analysis identified 117 DEGs in the SCN-CCI rat model, involved in various biological processes and pathways related to sciatica. PPI network analysis revealed hub genes, including Ly6g6e, which exhibited significant differential expression. QRT-PCR and Western blot analysis confirmed the expression patterns of these hub genes. Pain behavior assessment demonstrated reduced pain thresholds and increased paw flinching responses in the SCN-CCI group. Furthermore, the SCN-CCI group showed upregulated expression of Ly6g6e, increased protein levels of Ly6g6e, CGRP, and NGF, as well as elevated levels of IL-1β, MCP-1, and IL-6, and microglial cell activation in the spinal dorsal horn. ELISA results confirmed the increased levels of IL-1β, MCP-1, and IL-6 in the spinal dorsal horn.

Conclusion

These comprehensive findings provide valuable insights into the SCN-CCI rat model, DEGs associated with sciatica, hub genes (Ly6g6e as promising targets), pain behavior changes and molecular alterations.
The food and beverage cues in digital marketing model: special considerations of social media, gaming, and livestreaming environments for food marketing and eating behavior research
Authors: Maksi Sara J. , Keller Kathleen L. , Dardis Frank , Vecchi Martina , Freeman Jason , Evans Rebecca K. , Boyland Emma , Masterson Travis D.
Published: February 2024

Digital marketing to children, teens, and adults contributes to substantial exposure to cues and persuasive messages that drive the overconsumption of energy dense foods and sugary beverages. Previous food marketing research has focused on traditional media, but less is known about how marketing techniques translate within digital platforms, such as social media, livestreaming, and gaming. Building upon previous theories and models, we propose a new model entitled food and beverage cues in digital marketing (FBCDM). The FBCDM model specifies key marking elements and marketing integration strategies that are common on digital platforms and are hypothesized to enhance the effects of advertising and incentive sensitization process. FBCDM also categorizes measurable outcomes into three domains that include brand, food, and social outcomes. Additionally, repeated marketing exposure and the resulting outcomes are hypothesized to have long term consequences related to consumer markets, consumption behavior, culture, and health. We include a discussion of what is currently known about digital marketing exposure within the outcome domains, and we highlight gaps in research including the long-term consequences of digital marketing exposure. The FBCDM model provides a conceptual framework to guide future research to examine the digital marketing of food and beverages to children and adolescents in order to inform government and industry policies that restrict the aggressive marketing of products associated with obesity and adverse diet related outcomes.
Antioxidant, aroma, and sensory characteristics of Maillard reaction products from Urechis unicinctus hydrolysates: development of food flavorings
Authors: Du Mengdi , Yu Wei , Ding Ning , Jian Mengqi , Cheng Yongqiang , Gan Jing
Published: February 2024

To develop food flavorings with a delicious taste and an anti-oxidation effect, in this study, the glucose Maillard reaction was used for hydrolysates of Urechis unicinctus. The various biological activities of Maillard reaction products (MRPs) and their antioxidant capacity were evaluated. The results showed that the unique fishy odor substances of seafood in MRPs were reduced, indicating that the Maillard reaction improved the flavor of the hydrolysate of Urechis unicinctus. Meanwhile, MRPs exhibited more competitive radical scavenging activities compared to the hydrolysate. Moreover, MRPs demonstrated a considerable potential to protect against 2,2′-Azobis (2-methylpropionamidine) dihydrochloride (AAPH)-induced oxidative stress in a cell model in vitro and in a zebrafish model in vivo. Finally, a novel food flavoring was produced with MRPs as raw material, while the sensory qualities were deemed acceptable. In consequence, during industrial production, MRPs of Urechis unicinctus hydrolysate act as a high-quality raw material for functional flavorings and provide an effective way for the utilization of marine resources.
High-risk prostate cancer treated with a stereotactic body radiation therapy boost following pelvic nodal irradiation
Authors: Lischalk Jonathan W. , Akerman Meredith , Repka Michael C. , Sanchez Astrid , Mendez Christopher , Santos Vianca F. , Carpenter Todd , Wise David , Corcoran Anthony , Lepor Herbert , Katz Aaron , Haas Jonathan A.
Published: February 2024

Purpose

Modern literature has demonstrated improvements in long-term biochemical outcomes with the use of prophylactic pelvic nodal irradiation followed by a brachytherapy boost in the management of high-risk prostate cancer. However, this comes at the cost of increased treatment-related toxicity. In this study, we explore the outcomes of the largest cohort to date, which uses a stereotactic body radiation therapy (SBRT) boost following pelvic nodal radiation for exclusively high-risk prostate cancer.

Methods and materials

A large institutional database was interrogated to identify all patients with high-risk clinical node-negative prostate cancer treated with conventionally fractionated radiotherapy to the pelvis followed by a robotic SBRT boost to the prostate and seminal vesicles. The boost was uniformly delivered over three fractions. Toxicity was measured using the Common Terminology Criteria for Adverse Events (CTCAE) version 5.0. Oncologic outcomes were assessed using the Kaplan–Meier method. Cox proportional hazard models were created to evaluate associations between pretreatment characteristics and clinical outcomes.

Results

A total of 440 patients with a median age of 71 years were treated, the majority of whom were diagnosed with a grade group 4 or 5 disease. Pelvic nodal irradiation was delivered at a total dose of 4,500 cGy in 25 fractions, followed by a three-fraction SBRT boost. With an early median follow-up of 2.5 years, the crude incidence of grade 2+ genitourinary (GU) and gastrointestinal (GI) toxicity was 13% and 11%, respectively. Multivariate analysis revealed grade 2+ GU toxicity was associated with older age and a higher American Joint Committee on Cancer (AJCC) stage. Multivariate analysis revealed overall survival was associated with patient age and posttreatment prostate-specific antigen (PSA) nadir.

Conclusion

Utilization of an SBRT boost following pelvic nodal irradiation in the treatment of high-risk prostate cancer is oncologically effective with early follow-up and yields minimal high-grade toxicity. We demonstrate a 5-year freedom from biochemical recurrence (FFBCR) of over 83% with correspondingly limited grade 3+ GU and GI toxicity measured at 3.6% and 1.6%, respectively. Long-term follow-up is required to evaluate oncologic outcomes and late toxicity.
Clinical characteristics and risk factors analysis of 505 cases of infusion reactions in a tertiary hospital
Authors: Yin Weiwei , Wen Bingqin , Wang Guoan , Wang Zhipeng , Kong Xuetao , Wu Yaozhou , Meng Xiao , Ou Xinyi , Wei Li , Yu Pengjiu
Published: February 2024

Background: The clinical characteristics and risk factors of infusion reactions (IRs) are inadequately described in clinical practice due to underreported cases. In the present study, we reported the current status of IRs based on an in-hospital pharmacovigilance database of a tertiary care hospital.

Methods: Our study conducted a retrospective analysis of drug-induced IRs recorded at an in-hospital pharmacovigilance center between January 2015 to December 2019. The descriptive statistical analysis encompassed main causative agents, clinical manifestations, organ/system involvement and outcome. The severity of IRs was assessed with reference to the CTCAE version 5.0 criteria and we investigated risk factors associated with severe IRs.

Results: During the study period, a total of 505 cases of inpatient drug-induced IRs were detected, of which 79.2% (400 cases) were classified as general IRs and 20.8% (105 cases) were categorized as severe IRs. The primary drugs responsible for these reactions were antibiotics (23%, 116 cases), with piperacillin sodium—sulbactam sodium being the most prevalent, followed by antineoplastic agents (18.4%, 93 cases) and traditional Chinese medicine injections (TCMIs) (12.9%, 65 cases). The administration of cefoperazone - sulbactam, mannatide, Shenqi Fuzheng, elemene, and diterpene ginkgolides meglumine resulted in a higher incidence of critical IRs. Among all cases of IRs, 43.2%, 41.2%, and 23.4% showed signs and symptoms of circulation, skin mucosa, and respiratory organs/systems, respectively. 9.1% of cases experienced systemic damage, while 7.1% and 5.9% of cases reported neurological and gastrointestinal related adverse reactions, respectively. The multivariate analysis revealed that alcohol consumption (OR = 2.389%, 95% CI 1.141–5.002, p = 0.021), age over 65 (OR = 1.814%, 95% CI 1.052–3.127, p = 0.032) and the utilization of contrast media (OR = 4.072%, 95% CI 1.903–8.713, p < 0.001) were identified as risk factors for the development of severe IRs.

Conclusion: Understanding the clinical characteristics of IRs helps to implement effective pharmaceutical monitoring and appropriate preventive measures for susceptible populations with risk factors.
Predicting the canopy conductance to water vapor of grapevines using a biophysical model in a hot and arid climate
Authors: Egipto Ricardo Jorge Lopes , Aquino Arturo , Andújar José Manuel
Published: February 2024

Canopy conductance is a crucial factor in modelling plant transpiration and is highly responsive to water stress. The objective of this study is to develop a straightforward method for estimating canopy conductance (gc) in grapevines. To predict gc, this study combines stomatal conductance to water vapor (gsw) measurements from grapevine leaves, scaled to represent the canopy size by the leaf area index (LAI), with atmospheric variables, such as net solar radiation (Rn) and air vapor pressure deficit (VPD). The developed model was then validated by comparing its predictions with gc values calculated using the inverse of the Penman Monteith equation. The proposed model demonstrates its effectiveness in estimating the gc, with the highest root-mean-squared-error (RMSE=1.45x10−4m.s−1) being lower than the minimum gc measured in the field (gc obs=0.0005 m.s−1). The results of this study reveal the significant influence of both VPD and gsw on grapevine canopy conductance.
A lighthearted approach to mindfulness: development and evaluation of a humor-enriched mindfulness-based program in a randomized trial
Authors: Kastner Christian T.
Published: February 2024

Objectives

Recently, research on mindfulness and humor has begun to spark interest, both being essential contributors to well-being. This article proposes that forms of humor that share intention and attitudinal foundations with mindfulness, constitute the foundation of a mindful-humorous perspective and mindful humor. Once intention and attitude are in alignment, shared mechanisms underlying a mindful-humorous perspective may lead to synergetic effects (e.g., reinforcing a shift in perspective and attitudinal foundations) as summarized in the proposed mindful humor filter model (MHFM). Based on this theoretical framework, the humor-enriched mindfulness-based program (HEMBP) was developed and evaluated for validity and efficacy in a randomized controlled trial as a first step to test the newly introduced model.

Methods

A total of 60 participants were randomly allocated to either the HEMBP or a wait-list control group. Participants' mindfulness, comic styles (e.g., benevolent humor, sarcasm, and cynicism), psychological well-being, life satisfaction, stress, and gelotophobia (fear of being laughed at) were assessed 1 week before and after training, and at a 1-month follow-up. Linear mixed-effects models were fitted to model changes in outcome variables over time.

Results

The HEMBP was effective in increasing mindfulness, benevolent humor, and psychological well-being, and in reducing sarcasm, cynicism, stress, and gelotophobia, compared to the control group.

Conclusion

Results support the validity and efficacy of the HEMBP as a promising approach for improving well-being and reducing stress. The HEMBP broadens the scope of existing mindfulness-based programs by cultivating a mindful-humorous outlook on life, facilitating mindfulness practice and insights, and fostering positive emotions and relationships with others through mindful humor. Results are also suggestive of the validity of the mindful humor filter model.
The effects of awe on interpersonal forgiveness: the mediating role of small-self
Authors: Liao Suxia , Liu Yichang , Yuan Bo
Published: February 2024

Awe could increase prosocial behavior, but little is known about its effects on interpersonal forgiveness. This study aims to explore the potential impact of awe on interpersonal forgiveness and the underlying mechanism of this process, using a combination of questionnaires, economic game and computational modeling. In Study 1, we utilized Trait Awe Scale (TAS) and Forgiveness Trait Scale (FTS) to examine the association between trait awe and trait forgiveness. In Study 2, we employed pre-screened video to induce awe, happy and neutral emotions, then evaluated the effects of induced awe on small-self and interpersonal forgiveness in hypothetical interpersonal offensive situations (Study 2a) and two economic interaction situations (Study 2b). Results from Study 1 indicate that there is a positive correlation between trait awe and trait forgiveness. Study 2 reveal that awe can enhance interpersonal forgiveness in both interpersonal conflict situations and economic interaction situations, and this effect is mediated by the sense of small-self elicited by awe. Overall, these findings contribute to our understanding of the potential impact of awe on interpersonal forgiveness and provide valuable insights into the mechanisms through which awe may influence forgiveness. Further research in this area could help to elucidate the potential applications of awe-based interventions in promoting forgiveness and positive social interactions.
Differentiating mindfulness-integrated cognitive behavior therapy and mindfulness-based cognitive therapy clinically: the why, how, and what of evidence-based practice
Authors: Francis Sarah E. B. , Shawyer Frances , Cayoun Bruno A. , Grabovac Andrea , Meadows Graham
Published: February 2024

It is important to be able to differentiate mindfulness-based programs in terms of their model, therapeutic elements, and supporting evidence. This article compares mindfulness-based cognitive therapy (MBCT), developed for relapse prevention in depression, and mindfulness-integrated cognitive behavior therapy (MiCBT), developed for transdiagnostic applications, on: (1) origins, context and theoretical rationale (why), (2) program structure, practice and, professional training (how), and (3) evidence (what). While both approaches incorporate behavior change methods, MBCT encourages behavioral activation, whereas MiCBT includes various exposure procedures to reduce avoidance, including a protocol to practice equanimity during problematic interpersonal interactions, and a compassion training to prevent relapse. MBCT has a substantial research base, including multiple systematic reviews and meta-analyses. It is an endorsed preventative treatment for depressive relapse in several clinical guidelines, but its single disorder approach might be regarded as a limitation in many health service settings. MiCBT has a promising evidence base and potential to make a valuable contribution to psychological treatment through its transdiagnostic applicability but has not yet been considered in clinical guidelines. While greater attention to later stage dissemination and implementation research is recommended for MBCT, more high quality RCTs and systematic reviews are needed to develop the evidence base for MiCBT.
Factors influencing second language learning based on the research of Lightbown and Spada
Authors: Qiao Chuandai
Published: February 2024

Deep insights into the factors influencing second language learners can positively impact learners’ self-intervention and guide language teachers in selecting appropriate teaching materials and strategies. Drawing from Lightbown and Spada’s framework encompassing motivation, aptitude, personality, intelligence, and learner preferences, this paper examines the relationship between individual learners’ characteristics and second language learning effects across five aspects by dialectically considering the research methods suitable for different learner variables. By incorporating recent research and offering practical implications, this paper has the potential to contribute valuable insights to both researchers and practitioners in the field of language education.
The hemophilia quality of life scale: a systematic review
Authors: Wang Xin , Li Zhixiao , Li Lele
Published: February 2024

Purpose

Quality of life refers to the degree of well-being a person feels. The development of a hemophilia-targeting quality of life scale is important for hemophiliacs and their treating physicians.

Methods

Textual analysis. First, a review of studies on quality of life, hemophilia and related quality of life scales was conducted; Subsequently, two rounds of systematic searches of the Springer database were conducted to filter the literature on universal quality of life scale studies and hemophilia-targeting quality of life scale studies by title and abstract, and then textual analysis was performed.

Results

The former included 77,456 articles, 26,117 chapters and 7,086 related bibliographies, while the latter initially retrieved 211 entries articles, 118 chapters and 43 related bibliographies. Through filtering, the former contains 22 documents, yielding 1,431 valid word stems, and the latter contains 9 documents, yielding 1,541 valid word stems.

Conclusion

While universal quality of life scales mostly fit into the broad framework of WHOQOL- BREF, the development of hemophilia-targeting quality of life scales inclines towards pains that patients suffer and technology advances in pharmaceutical. The current hemophilia QOL scales are mainly based on the HR-QoL, others mainly based on the HR-QoL as the master version. At the same time, the popularization of existing hemophilia quality of life scales in developing countries like China is not high, and the development of hemophilia quality of life scales is insufficient.
Effects of medical interventions on health-related quality of life in chronic disease – systematic review and meta-analysis of the 19 most common diagnoses
Authors: Riecke Franziska , Bauer Leandra , Polzer Hans , Baumbach Sebastian Felix , Neuerburg Carl , Böcker Wolfgang , Grill Eva , Saller Maximilian Michael
Published: February 2024

Introduction

The demographic shift leads to a tremendous increase in age-related diseases, which are often chronic. Therefore, a focus of chronic disease management should be set on the maintenance or even improvement of the patients’ quality of life (QoL). One indicator to objectively measure QoL is the EQ-5D questionnaire, which was validated in a disease- and world region-specific manner. The aim of this study was to conduct a systematic literature review and meta-analysis on the QoL across the most frequent chronic diseases that utilized the EQ-5D and performed a disease-specific meta-analysis for treatment-dependent QoL improvement.

Materials and methods

The most common chronic disease in Germany were identified by their ICD-10 codes, followed by a systematic literature review of these ICD-10 codes and the EQ-5D index values. Finally, out of 10,016 independently -screened studies by two persons, 538 studies were included in the systematic review and 216 studies in the meta-analysis, respectively.

Results

We found significant medium to large effect sizes of treatment effects, i.e., effect size >0.5, in musculoskeletal conditions with the exception of fractures, for chronic depression and for stroke. The effect size did not differ significantly from zero for breast and lung cancer and were significantly negative for fractures.

Conclusion

Our analysis showed a large variation between baseline and post-treatment scores on the EQ-5D health index, depending on the health condition. We found large gains in health-related quality of life mainly for interventions for musculoskeletal disease.

Systematic review registration

https://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42020150936, PROSPERO identifier CRD42020150936.
Differences in surgical outcomes between cervical goiter and retrosternal goiter: an international, multicentric evaluation
Authors: Cappellacci Federico , Canu Gian Luigi , Rossi Leonardo , De Palma Andrea , Mavromati Maria , Kuczma Paulina , Di Filippo Giacomo , Morelli Eleonora , Demarchi Marco Stefano , Brazzarola Paolo , Materazzi Gabriele , Calò Pietro Giorgio , Medas Fabio ,   our Mediastinal Goiter Study Collaborative Group
Published: February 2024

Introduction

Goiter is a common problem in clinical practice, representing a large part of clinical evaluations for thyroid disease. It tends to grow slowly and progressively over several years, eventually occupying the thoracic inlet with its lower portion, defining the situation known as retrosternal goiter. Total thyroidectomy is a standardized procedure that represents the treatment of choice for all retrosternal goiters, but when is performed for such disease, a higher risk of postoperative morbidity is variously reported in the literature. The aims of our study were to compare the perioperative and postoperative outcomes in patients with cervical goiters and retrosternal goiters undergoing total thyroidectomy.

Methods

In our retrospective, multicentric evaluation we included 4,467 patients, divided into two groups based on the presence of retrosternal goiter (group A) or the presence of a classical cervical goiter (group B).

Results

We found statistically significant differences in terms of transient hypoparathyroidism (19.9% in group A vs. 9.4% in group B, p < 0.001) and permanent hypoparathyroidism (3.3% in group A vs. 1.6% in group B, p = 0.035). We found no differences in terms of transient RNLI between group A and group B, while the occurrence of permanent RLNI was higher in group A compared to group B (1.4% in group A vs. 0.4% in group B, p = 0.037). Moreover, no differences in terms of unilateral RLNI were found, while bilateral RLNI rate was higher in group A compared to group B (1.1% in group A vs. 0.1% in group B, p = 0.015).

Discussion

Wound infection rate was higher in group A compared to group B (1.4% in group A vs. 0.2% in group B, p = 0.006). Based on our data, thyroid surgery for retrosternal goiter represents a challenging procedure even for highly experienced surgeons, with an increased rate of some classical thyroid surgery complications. Referral of these patients to a high-volume center is mandatory. Also, intraoperative nerve monitoring (IONM) usage in these patients is advisable.
Analysis of Parvovirus B19 persistence and reactivation in human heart layers
Authors: Badrinath Ashwin , Gardere Anais , Palermo Samantha L. , Campbell Kenneth S. , Kloc Anna
Published: February 2024

Heart disease is the leading cause of death worldwide. Myocarditis, or inflammation of the cardiac muscle, is estimated to cause up to 1.5 million cases annually, with viral infection being the most common disease culprit. Past studies have shown that Parvovirus B19 is routinely detected in endomyocardial biopsies. This virus has been linked to acute heart inflammation, which can cause cardiac muscle damage. However, because Parvovirus B19 can be found in the heart tissues in the absence of disease symptoms, it is unclear if the long-term presence of the virus contributes to, or initiates, heart disease. Here, we utilized a PCR-based detection assay to assess the presence of the B19V genome and its mRNA intermediates in human heart tissues. The analysis was carried out in three heart layers derived from one individual: epicardium, endocardium and myocardium. We showed the Parvovirus B19 genome presence variability in different heart layers. Similarly, viral transcriptional activity, assessed by the mRNA presence, was detected only in a few of the analyzed samples. Our results suggest that localized sites of Parvovirus B19 infection may exist within individual heart layers, which may have implication for the cardiac muscle inflammation.
Special Issue: “2022 and 2023 Selected Papers from Algorithms’ Editorial Board Members”
Authors: Werner Frank
Published: February 2024

This is the third edition of a Special Issue of Algorithms; it is of a rather different nature compared to other Special Issues in the journal, which are usually dedicated to a particular subject in the area of algorithms [...]
Numbers Do Not Lie: A Bibliometric Examination of Machine Learning Techniques in Fake News Research
Authors: Sandu Andra , Ioanăș Ioana , Delcea Camelia , Florescu Margareta-Stela , Cotfas Liviu-Adrian
Published: February 2024

Fake news is an explosive subject, being undoubtedly among the most controversial and difficult challenges facing society in the present-day environment of technology and information, which greatly affects the individuals who are vulnerable and easily influenced, shaping their decisions, actions, and even beliefs. In the course of discussing the gravity and dissemination of the fake news phenomenon, this article aims to clarify the distinctions between fake news, misinformation, and disinformation, along with conducting a thorough analysis of the most widely read academic papers that have tackled the topic of fake news research using various machine learning techniques. Utilizing specific keywords for dataset extraction from Clarivate Analytics’ Web of Science Core Collection, the bibliometric analysis spans six years, offering valuable insights aimed at identifying key trends, methodologies, and notable strategies within this multidisciplinary field. The analysis encompasses the examination of prolific authors, prominent journals, collaborative efforts, prior publications, covered subjects, keywords, bigrams, trigrams, theme maps, co-occurrence networks, and various other relevant topics. One noteworthy aspect related to the extracted dataset is the remarkable growth rate observed in association with the analyzed subject, indicating an impressive increase of 179.31%. The growth rate value, coupled with the relatively short timeframe, further emphasizes the research community’s keen interest in this subject. In light of these findings, the paper draws attention to key contributions and gaps in the existing literature, providing researchers and decision-makers innovative viewpoints and perspectives on the ongoing battle against the spread of fake news in the age of information.
Early Cannon Development in Females of the “Sanmartinero” Creole Bovine Breed
Authors: Salamanca-Carreño Arcesio , Parés-Casanova Pere M. , Vélez-Terranova Mauricio , Martínez-Correal Germán , Rangel-Pachón David E.
Published: February 2024

The variation in the dimensions of the body of living beings in relation to their size, function, or shape is known as allometry. Allometry studies allow the analysis of the function of body structures and their relationship with the performance and survival of breeds and species. The aim of this study was to describe the ontogenetic characteristics of the weight of certain bone elements of the Sanmartinero creole bovine breed, from an early age (6 months) until maturity. A total of 79 females of the Sanmartinero creole bovine breed with an age range of 0.5 to 10 years were studied. Body measurements were obtained individually using standard procedures: cannon perimeter, thoracic perimeter, body length, withers height, and body weight. Functional and production indices were obtained for animals aged more than 3 years (n = 53): corporal index, anamorphosis index, dactylo thoracic index, and the relative thickness of the cannon. The correlation between body weight and the cannon perimeter showed no trend towards a plateau. In the Sanmartinero creole bovine, therefore, a very early development of the cannon perimeter is detected, which could lead to bone fragility in heavier animals. Therefore, data suggest little harmony with appendicular bone development, i.e., with less robust cannons which are those necessary to support body mass. It is suggested that in genetic improvement programs of the Sanmartinero creole bovine, the measurement of the cannon perimeter, the withers height, and the thoracic perimeter should be considered.
A Multidimensional Evaluation of the Factors in the Animal Welfare Assessment Grid (AWAG) That Are Associated with, and Predictive of, Behaviour Disorders in Dogs
Authors: Malkani Rachel , Paramasivam Sharmini , Wolfensohn Sarah
Published: February 2024

Behavioural disorders in dogs are common and have severe welfare consequences for dogs. This study aimed to assess the factors that are significant and predictive of behaviour problems in dogs using the animal welfare assessment grid (AWAG) to further understand what factors influence their welfare. 177 AWAG assessments were undertaken across 129 dogs that clinicians deemed to have a behavioural disorder. Wilcoxon rank-sum tests were used to assess the difference in scores between dogs with behaviour disorders and a cohort of healthy dogs (n = 117). This analysis showed that all physical factors besides body condition, all procedural factors besides procedure pain, and all psychological, and environmental factors were significantly different between healthy dogs and dogs with behaviour disorders. Spearman rank correlation coefficient (RS) revealed several significant strong positive correlations including the procedural impact on the dog’s daily routine with aggression towards unfamiliar people and procedure pain, as well as other correlations between the dog’s behaviour during assessment with the frequency at which they encounter fears and anxieties, clinical assessment and procedure pain, and reaction to stressors and social interactions. These findings highlight the interdependent nature of the various influences of welfare. Logistic regression analysis identified that aggression towards the caregiver, fears and anxieties frequency, and choice, control, and predictability were all significant predictors of behaviour disorders. The findings have important implications for veterinary, behaviour, and animal welfare professionals as any changes across these factors may indicate poor welfare linked to emotional disorders in dogs.
Buffalo bbu-miR-493-5p Promotes Myoblast Proliferation and Differentiation
Authors: Zhang Liyin , Zhong Dandan , Yao Chengxuan , Liu Qingyou , Shi Deshun , Jiang Mingsheng , Wang Jian , Xiong Zhaocheng , Li Hui
Published: February 2024

In recent years, the meat and dairy value of buffaloes has become a major concern in buffalo breeding, and the improvement of buffalo beef quality is key to protecting buffalo germplasm resources and solving the problem of beef supply. MiRNAs play a significant role in regulating muscle development. However, the precise mechanism by which they regulate the development of buffalo skeletal muscles remains largely unexplored. In this study, we examined miRNA expression profiles in buffalo myoblasts during the proliferation and differentiation stages. A total of 177 differentially expressed miRNAs were identified, out of which 88 were up-regulated and 89 down-regulated. We focused on a novel miRNA, named bbu-miR-493-5p, that was significantly differentially expressed during the proliferation and differentiation of buffalo myoblasts and highly expressed in muscle tissues. The RNA-FISH results showed that bbu-miR-493-5p was primarily located in the cytoplasm to encourage buffalo myoblasts’ proliferation and differentiation. In conclusion, our study lays the groundwork for future research into the regulatory role of miRNAs in the growth of buffalo muscle.
Morphological Ontogeny, Ecology, and Biogeography of Fuscozetes fuscipes (Acari, Oribatida, Ceratozetidae)
Authors: Seniczak Stanisław , Seniczak Anna , Jordal Bjarte H.
Published: February 2024

The systematic status of Fuscozetes Sellnick, 1928, is not clear in the literature. Therefore, the morphological ontogeny of F. fuscipes (C.L. Koch, 1844), the type species of this genus, was investigated and compared with its congeners in this study, and a new diagnosis of Fuscozetes is given. The juveniles of F. fuscipes are light brown, with a brown prodorsum, sclerites, epimeres, and legs. In all juveniles, a humeral organ and a humeral macrosclerite are present. The gastronotum of the larva has 12 pairs of setae (h3 is present), whereas the nymphs have 15 pairs. In the larva, the gastronotal shield is weakly developed, and most gastronotal setae are short except for a slightly longer h2. Most of the gastronotal setae are inserted on the microsclerites except for h3, and several other macrosclerites and many microsclerites are present on the hysterosoma. In the nymphs, the gastronotal shield is well developed, with 10 pairs of setae (d-, l-, and h-series, and p1), and setae p2 and p3 are located on a large posteroventral macrosclerite. In all the instars, femora I and II are oval in cross-section, without a large ventral carina. Mitochondrial COI sequence data revealed a deep split between the Nearctic and Palearctic populations of F. fuscipes, and a less, but significant, divergence within each continent. These strong geographical barriers were contrasted with multiple cases of shared haplotypes over long distances in the Palearctic, indicating high migration rates in modern times.
Effect of a 14-Day Period of Heat Acclimation on Horses Using Heated Indoor Arenas in Preparation for Tokyo Olympic Games
Authors: Munsters Carolien , Siegers Esther , Sloet van Oldruitenborgh-Oosterbaan Marianne
Published: February 2024

To optimise the performance and welfare of horses during equestrian competitions in hot climates, it is advised to acclimate them to the heat. The effects of training in a heated indoor arena were studied. Four Olympic horses (13.3 ± 2.2 years; three eventers, one para-dressage horse) were trained for 14 consecutive days in a heated indoor arena (32 ± 1 °C; 50–60% humidity) following their normal training schedule in preparation for the Tokyo Olympic games. Standardised exercise tests (SETs) were performed on Day 1 and Day 14, measuring heart rate (HR; bpm), plasma lactate concentration (LA; mmol/L), deep rectal temperature (Trec; °C), sweat loss (SL; L), and sweat composition (K+, Cl− and Na+ concentration). The data were analysed using linear mixed models. The Trec and HR were significantly decreased after acclimation (estimate: −0.106, 95% CI −0.134, −0.078; estimate: −4.067, 95% CI −7.535, −0.598, respectively). Furthermore, for all the horses, the time taken to reach their peak Trec and heat storage increased, while their LA concentrations decreased. The SL, Cl−, and Na+ concentrations decreased in three out of the four horses. Conclusions: Fourteen days of normal training in a heated indoor arena resulted in a reduction in cardiovascular and thermal strain. This is advantageous because it shows that elite sport horses can be acclimated while training as usual for a championship.