bluuebunny/crossref_metadata_2025 huggingface dataset:
Diet Hierarchies Guide Temporal-Spatial Variation in Drosophila suzukii Resource Use
Authors: Stockton Dara G. , Loeb Gregory M.
Published: February 2022

Among insects, female oviposition preferences are critical to understanding the evolutionary dynamics between herbivores and hosts. Previous studies have shown Drosophila resource use has a strong genetic basis, although there is evidence that preferences are adaptable given isolation from ancestral hosts. Given the high degree of adaptability and behavioral plasticity of invasive species, we were interested in the mechanisms affecting host preferences of the invasive fruit fly, Drosophila suzukii, which in recent years has developed a nearly global range infesting small fruit crops. We studied the diet hierarchies of D. suzukii using a combination of laboratory and field assays designed to assess how female oviposition host choice differs given the availability of, and experience with, different fruit and non-fruit hosts. We found that host preferences did not shift over time and flies reared on two differential isolated diets up to F5 behaved and performed similarly regardless of diet lineage. Rather, female host choice appeared guided by a fixed hierarchical system of host preferences. Raspberry was more preferred to mushroom, which was more preferred to goose manure. However, if preferred resources were absent, the use of less-preferred resources was compensatory. We suggest that among niche specialists, such as D. suzukii, these hierarchies may support a bet-hedging strategy, rather than multiple-niche polymorphism, allowing for niche separation during periods of increased competition, while maintaining more diverse, ancestral feeding behaviors when preferred resources are scarce.
Transcriptomic Mapping of Non-Small Cell Lung Cancer K-RAS p.G12C Mutated Tumors: Identification of Surfaceome Targets and Immunologic Correlates
Authors: Alcaraz-Sanabria Ana , Cabañas Morafraile Esther , Fernández-Hinojal Gonzalo , Velasco Guillermo , Pérez-Segura Pedro , Pandiella Atanasio , Győrffy Balázs , Ocaña Alberto
Published: February 2022

Targeting K-RAS-mutant non-small cell lung cancer (NSCLC) with novel inhibitors has shown promising results with the recent approval of sotorasib in this indication. However, progression to this agent is expected, as it has previously been observed with other inhibitors. Recently, new immune therapeutics, including vectorized compounds with antibodies or modulators of the host immune response, have demonstrated clinical activity. By interrogating massive datasets, including TCGA, we identified genes that code for surface membrane proteins that are selectively expressed in K-RAS mutated NSCLC and that could be used to vectorize novel therapies. Two genes, CLDN10 and TMPRSS6, were selected for their clear differentiation. In addition, we discovered immunologic correlates of outcome that were clearly de-regulated in this particular tumor type and we matched them with immune cell populations. In conclusion, our article describes membrane proteins and immunologic correlates that could be used to better select and optimize current therapies.
Keratin 17 Promotes T Cell Response in Allergic Contact Dermatitis by Upregulating C–C Motif Chemokine Ligand 20
Authors: Luo Yixin , Zhu Zhenlai , Li Bing , Bai Xiaocui , Fang Hui , Qiao Pei , Chen Jiaoling , Zhang Chen , Zhi Dalong , Dang Erle , Wang Gang
Published: February 2022

Allergic contact dermatitis (ACD) is a delayed-type hypersensitivity response to skin contact allergens in which keratinocytes are critical in the initiation of early responses. Keratin 17 (K17) is a cytoskeletal protein inducible under stressful conditions and regulates multiple cellular processes, especially in skin inflammatory diseases; however, knowledge regarding its contribution to ACD pathogenesis remains ill defined. In the present study, we clarified the proinflammatory role of K17 in an oxazolone (OXA)-induced contact hypersensitivity (CHS) murine model and identified the underlying molecular mechanisms. Our results showed that K17 was highly expressed in the lesional skin of ACD patients and OXA-induced CHS mice. Mice lacking K17 exhibited alleviated OXA-induced skin inflammation, including milder ear swelling, a reduced frequency of T cell infiltration, and decreased inflammatory cytokine levels. In vitro, K17 stimulated and activated human keratinocytes to produce plenty of proinflammatory mediators, especially the chemokine CCL20, and promoted keratinocyte-mediated T cell trafficking. The neutralization of CCL20 with a CCL20-neutralizing monoclonal antibody significantly alleviated OXA-induced skin inflammation in vivo. Moreover, K17 could translocate into the nucleus of activated keratinocytes through a process dependent on the nuclear-localization signal (NLS) and nuclear-export signal (NES) sequences, thus facilitating the activation and nuclear translocation of signal transducer and activator of transcription 3 (STAT3), further promoting the production of CCL20 and T cell trafficking to the lesional skin. Taken together, these results highlight the novel roles of K17 in driving allergen-induced skin inflammation and suggest targeting K17 as a potential strategy for ACD.
In silico Analysis of SARS-CoV-2 ORF8-Binding Proteins Reveals the Involvement of ORF8 in Acquired-Immune and Innate-Immune Systems
Authors: Takatsuka Hisashi , Fahmi Muhamad , Hamanishi Kotono , Sakuratani Takuya , Kubota Yukihiko , Ito Masahiro
Published: February 2022

SARS-CoV-2 is the causative agent of a new type of coronavirus infection, COVID-19, which has rapidly spread worldwide. The overall genome sequence homology between SARS-CoV-2 and SARS-CoV is 79%. However, the homology of the ORF8 protein between these two coronaviruses is low, at ~26%. Previously, it has been suggested that infection by the ORF8-deleted variant of SARS-CoV-2 results in less severe symptoms than in the case of wild-type SARS-CoV-2. Although we found that ORF8 is involved in the proteasome autoimmunity system, the precise role of ORF8 in infection and pathology has not been fully clarified. In this study, we determined a new network of ORF8-interacting proteins by performing in silico analysis of the binding proteins against the previously described 47 ORF8-binding proteins. We used as a dataset 431 human protein candidates from Uniprot that physically interacted with 47 ORF8-binding proteins, as identified using STRING. Homology and phylogenetic profile analyses of the protein dataset were performed on 446 eukaryotic species whose genome sequences were available in KEGG OC. Based on the phylogenetic profile results, clustering analysis was performed using Ward's method. Our phylogenetic profiling showed that the interactors of the ORF8-interacting proteins were clustered into three classes that were conserved across chordates (Class 1: 152 proteins), metazoans (Class 2: 163 proteins), and eukaryotes (Class 3: 114 proteins). Following the KEGG pathway analysis, classification of cellular localization, tissue-specific expression analysis, and a literature study on each class of the phylogenetic profiling cluster tree, we predicted that the following: protein members in Class 1 could contribute to COVID-19 pathogenesis via complement and coagulation cascades and could promote sarcoidosis; the members of Class 1 and 2, together, may contribute to the downregulation of Interferon-β; and Class 3 proteins are associated with endoplasmic reticulum stress and the degradation of human leukocyte antigen.
Modeling Progression of Single Cell Populations Through the Cell Cycle as a Sequence of Switches
Authors: Zinovyev Andrei , Sadovsky Michail , Calzone Laurence , Fouché Aziz , Groeneveld Clarice S. , Chervov Alexander , Barillot Emmanuel , Gorban Alexander N.
Published: February 2022

Cell cycle is a biological process underlying the existence and propagation of life in time and space. It has been an object for mathematical modeling for long, with several alternative mechanistic modeling principles suggested, describing in more or less details the known molecular mechanisms. Recently, cell cycle has been investigated at single cell level in snapshots of unsynchronized cell populations, exploiting the new methods for transcriptomic and proteomic molecular profiling. This raises a need for simplified semi-phenomenological cell cycle models, in order to formalize the processes underlying the cell cycle, at a higher abstracted level. Here we suggest a modeling framework, recapitulating the most important properties of the cell cycle as a limit trajectory of a dynamical process characterized by several internal states with switches between them. In the simplest form, this leads to a limit cycle trajectory, composed by linear segments in logarithmic coordinates describing some extensive (depending on system size) cell properties. We prove a theorem connecting the effective embedding dimensionality of the cell cycle trajectory with the number of its linear segments. We also develop a simplified kinetic model with piecewise-constant kinetic rates describing the dynamics of lumps of genes involved in S-phase and G2/M phases. We show how the developed cell cycle models can be applied to analyze the available single cell datasets and simulate certain properties of the observed cell cycle trajectories. Based on our model, we can predict with good accuracy the cell line doubling time from the length of cell cycle trajectory.
Neural Correlates of Physical Activity Moderate the Association Between Problematic Mobile Phone Use and Psychological Symptoms
Authors: Zou Liwei , Wu Xiaoyan , Tao Shuman , Yang Yajuan , Zhang Qingjun , Hong Xuedong , Xie Yang , Li Tingting , Zheng Suisheng , Tao Fangbiao
Published: February 2022

Background

Despite the evidence of an association between problematic mobile phone use (PMPU) and psychological symptoms, a few studies explore whether physical activity (PA) could moderate the effect of PMPU on psychological symptoms and its neural substrates. The aim of this study was to examine the association between PMPU and psychological symptoms in late adolescents, along with the potential moderating effect of PA and neural basis by brain gray matter volume (GMV).

Methods

A total of 251 college students reported on their PMPU, PA, and psychological symptoms and subsequently underwent structural magnetic resonance imaging to explore the neural basis of their PA characteristics. A multiple regression model was performed to detect brain GMV associated with PA by the voxel-based morphometry (VBM) method. Moderating analysis was conducted using PROCESS macro in the SPSS software.

Results

Behavioral results showed that PMPU was correlated to depression, anxiety, and stress, and PA has significantly moderated the association between PMPU with depression, anxiety, and stress. The VBM analysis showed that PA was correlated to GMV of the right fusiform gyrus (FFG), left precuneus (PCUN), left insula (INS), and left triangular part of inferior frontal gyrus (IFGtriang). Moreover, GMV of the left INS moderated the relationship between PMPU and depression.

Conclusion

This study has shed light on the neural perspective of PA that moderates the relationship between PMPU and depressive symptom.
Impaired Nutritional Condition After Stroke From the Hyperacute to the Chronic Phase: A Systematic Review and Meta-Analysis
Authors: Huppertz Viviënne , Guida Sonia , Holdoway Anne , Strilciuc Stefan , Baijens Laura , Schols Jos M. G. A. , van Helvoort Ardy , Lansink Mirian , Muresanu Dafin F.
Published: February 2022

Background

Malnutrition is common after stroke and can affect rehabilitation and healthcare costs. A comprehensive overview of stroke patients' nutritional condition from the hyperacute to the chronic phase is lacking. This systematic review aimed to investigate the prevalence of impaired nutritional condition (INC) across the continuum of care in specific phases after stroke.

Methods

CAB ABSTRACTS, Embase, MEDLINE, were used to collect studies published between 01-01-1999 and 26-08-2020. Primary and secondary outcomes were prevalence of INC and prevalence of malnutrition, respectively. Exploratory outcomes were prevalence of INC at follow-up, nutritional examination methods, prevalence of dysphagia, stroke severity, adverse events, and continent-specific prevalence of INC. A random-effects meta-analysis model was used to estimate the phase-specific pooled prevalence of INC and malnutrition.

Results

The dataset consisted of 78 study groups selected over a total of 1,244 identified records. The pooled prevalence of INC and malnutrition were 19% (95%CI:7–31) (N = 4) and 19% (95%CI:9–29) (N = 3), 34% (95%CI:25–43) (N = 34) and 26% (95%CI:18–35) (N = 29), 52% (95%CI:43–61) (N = 34) and 37% (95%CI:28–45) (N = 31), 21% (95%CI:12–31) (N = 3) and 11% (95%CI:0–24) (N = 3) and 72% (95%CI:41–100) (N = 3) and 30% (95%CI:0–76) (N = 2) in the hyperacute, acute, early subacute, late subacute, and chronic phase, respectively.

Conclusion

INC and malnutrition are highly prevalent in all stages of stroke care. Since malnutrition has been shown to negatively affect clinical outcomes, mortality, and overall healthcare expenditure in stroke survivors, it is essential to examine and monitor the nutritional status of stroke patients throughout their care journey to guide and plan, timely nutritional support and dietary modification.
The Effect of Inflammatory Markers on the Survival of Advanced Gastric Cancer Patients Who Underwent Anti-Programmed Death 1 Therapy
Authors: Qu Ziting , Wang Qianling , Wang Hui , Jiao Yang , Li Min , Wei Wei , Lei Yu , Zhao Zhiyan , Zhang Tengteng , Zhang Yiyin , Gu Kangsheng
Published: February 2022

Purpose

This clinical study sought to determine whether the levels of inflammatory markers predicted the survival of advanced gastric cancer (AGC) patients who underwent anti-programmed death 1 (PD-1) therapy.

Methods

Using AGC patient plasma samples and baseline characteristics, we investigated the specific value of inflammatory markers in AGC from a clinical perspective in immunotherapy.

Results

One hundred and six patients with AGC who underwent anti-PD-1 therapy were enrolled in this study between 20 July 2019 and 16 March 2021. A significant decrease in NLR, dNLR, and SII was noticed among the PR (P=0.023; P=0.036; P=0.001), SD (P=0.048; P=0.022; P=0.023), ORR (P=0.021; P=0.032; P=0.001), and DCR (P=0.003; P=0.001; P<0.001) groups after anti-PD-1 therapy. Additionally, a significant decline of PLR was also observed in PR (P=0.010), ORR (P=0.007), and DCR (P=0.005) after anti-PD-1 therapy. Only MLR levels increased significantly at the time of anti-PD-1 immunotherapy the failure compared to baseline (P=0.039). And statistically significant elevations in NLR (P=0.001), MLR (P=0.020), dNLR (P=0.002), and SII (P=0.019) were found in failure of anti-PD-1 treatment compared to optimal efficacy in AGC patients. In first-line treatment, the number of metastatic sites (P=0.001) was an independent prognostic factor for PFS, and peritoneal metastases (P=0.004) and platelet-to-lymphocyte ratio (PLR) level (P=0.014) were independent prognostic predictors of OS according to Cox regression analysis. In second-line or posterior treatment, the number of metastatic sites (P=0.007), ECOG (P=0.011), and PLR level (P=0.033) were independent prognostic factors for PFS in AGC patients, and the number of metastatic sites (P=0.003), differentiation (P=0.030), and NLR level (P<0.001) were independent prognostic factors for OS according to Cox regression analysis.

Conclusions

NLR, PLR, MLR, dNLR, and SII can reflect the short-term efficacy of immunotherapy in patients who underwent anti-PD-1 therapy with AGC. PLR is an independent prognostic factor for OS in AGC patients receiving first-line immunotherapy and PFS in those receiving second-line or posterior immunotherapy. And NLR was an independent prognostic factor for OS in AGC patients receiving second-line or posterior immunotherapy. The number of metastatic sites was significantly associated with the prognosis of AGC patients who received immunotherapy.
Decylubiquinone Inhibits Colorectal Cancer Growth Through Upregulating Sirtuin2
Authors: Li Jinlian , Zheng Shuting , Cheng Ting , Li Yuanyuan , Mai Xiaobin , Jiang Guangchun , Yang Yongxia , Zhang Qianqian , Li Jiangchao , Zheng Lingyun , Wang Lijing , Qi Cuiling
Published: February 2022

Colorectal cancer (CRC) is one of the leading causes of cancer-related death worldwide. Decylubiquinone (DUb), a coenzyme Q10 analog, was reported to inhibit breast cancer growth and metastasis by us. However, the influence of DUb on CRC remains unclear. Herein, we found that DUb significantly inhibited CRC growth in the patient-derived xenograft (PDX) and CT26 xenograft models. DUb was further identified to significantly suppress CRC cell proliferation, colony formation, migration and invasion in a dose-dependent manner, while not inhibiting CRC cell apoptosis from flow cytometry assay. Sirtuin2 (SIRT2), a member of the sirtuin protein family, plays a critical role in growth and metastasis in various cancers. Moreover, DUb inhibited CRC progression by upregulating SIRT2. These findings reveal that DUb has the potential to a novel drug for the treatment of CRC by inhibiting CRC cell proliferation.
Understanding the Influence of Consumers’ Perceived Value on Energy-Saving Products Purchase Intention
Authors: Luo Biao , Li Liru , Sun Ying
Published: February 2022

Since rapid economic growth has led to the overuse of natural resources and environmental degradation, increasing attention has been paid to environmental problems. This study aims to explore the relationship between consumers’ perceived value and satisfaction, and energy-saving products purchase intention was investigated using appraisal-emotional response-coping theory. Moreover, this study further investigates these relationships in different consumer groups. In total, 399 questionnaires were collected online and offline, and results though structural equation modeling analysis show that functional, emotional, conditional, and green value have a positive effect on consumer satisfaction and thereby promote the intention to purchase energy-saving products. However, social value is not significant for consumer satisfaction. Perceived value influences consumer satisfaction and varies among different consumers according to the results of multigroup structural equation modeling analysis. These results have practical significance for the design and marketing of energy-saving products.
Attention Performance Correlated With White Matter Structural Brain Networks in Shift Work Disorder
Authors: Ning Yanzhe , Fang Meng , Zhang Yong , Feng Sitong , Feng Zhengtian , Liu Xinzi , Li Kuangshi , Jia Hongxiao
Published: February 2022

Neuroimaging studies have revealed that shift work disorder (SWD) affected the functional connectivity in specific brain regions and networks. However, topological disruptions in the structural connectivity of the white matter (WM) networks associated with attention function remain poorly understood. In the current study, we recruited 33 patients with SWD and 29 matched healthy subjects. The attention network test (ANT) was employed to investigate the efficiency of alerting, orienting, and executive control networks. The diffusion tensor imaging (DTI) tractography was used to construct the WM structural networks. The graph theory analysis was applied to detect the alterations of topological properties of structural networks. Our results showed lower alerting effect and higher executive effect for patients with SWD. Using the link-based analysis, 15 altered connectivity matrices (lower fiber numbers) were found between the two groups. Meanwhile, the graph theoretical analysis showed that the global efficiency and characteristic path length within SWD patients declined in contrast with the healthy controls. Furthermore, a significantly negative correlation was found between the executive effect and global network efficiency. Our findings provide the new insights into the fundamental architecture of interregional structural connectivity underlying attention deficits in SWD, which may be a potential biomarker for SWD.
Designing and Piloting of a Mobile Learning Curriculum for Quality Point-Of-Care Diagnostics Services in Rural Clinics of KwaZulu-Natal, South Africa
Authors: Chamane Nkosinothando , Thompson Rowan , Goldstone Simon , Mashamba-Thompson Tivani Phosa
Published: February 2022

Background

The use of mobile technology has been reported to help improve access to education for people in remote areas. However, there is limited evidence of its adoption in resource-limited settings. The aim of this study was to utilize stakeholder generated ideas to design and pilot a mobile learning curriculum, with the purpose of facilitating training to improve the quality of point-of-care diagnostics services in KwaZulu-Natal (KZN) rural clinics.

Methods

Nominal Group Technique was employed to enable collaboration with stakeholders in designing and piloting of a POC diagnostics curriculum. Stakeholders were selected from 11 KZN districts to participate in a clinic-based piloting of the curriculum using an online application. The application was designed in collaboration with a teacher training institute in Durban. Moodle was used as an established reliable online learning management system. During piloting, quantitative and qualitative data were generated and analyzed using descriptive statistics and content analysis.

Findings and Conclusion

Guided by the Nominal Group Technique results, five delivery modes for curriculum content through Mobile Learning were generated. An interactive course page was created on the Moodle site, titled:Quality HIV Point of Care Diagnostics Curriculum Delivery for Nurses in Rural Areas. The course content consisted of three teaching units, activities, an online quiz and an online survey. An analytic-algorithm built into the online course enabled monitoring of participation and assessment outcomes automatically. At piloting, 64% of the invited representative clinics were able to access the course, with 47% meeting the course completion requirements. All the participants achieved the set pass mark of 75% with an average of 87%. The activity completion report showed that topics presented through images, videos and simple text were accessed more than those presented as attachments of national documents. Despite poor network coverage and limited access to mobile technology, exacerbated by Covid-19 related restrictions, Point of care diagnostics Mobile Learning curriculum was well-received in participating rural clinics. Recommendations relating to course improvement and access, included extending collaboration with specialists in eHealth systems development and with South African cell phone network providers.
Assessment of the 2006 Abuja Fertilizer Declaration With Emphasis on Nitrogen Use Efficiency to Reduce Yield Gaps in Maize Production
Authors: Winnie Ntinyari , Giweta Mekonnen , Gweyi-Onyango Joseph , Mochoge Benson , Mutegi James , Nziguheba Generose , Masso Cargele
Published: February 2022

The Abuja Fertilizer Declaration in 2006 recommended the increase of fertilizer use from the current practice for Sub-Saharan Africa (SSA) to achieve food sufficiency and improve soil fertility status. However, the current recommended rates of fertilizer have not been evaluated for specific crops on their potential to reduce the yield gap and optimize nitrogen use efficiency (NUE). In this study, with nitrogen (N) being a significant yield-determinant nutrient, four N use scenarios were drawn from existing recommendations and were evaluated under field conditions for maize crops in two catchments of the Lake Victoria basin. The scenarios included Business as Usual (BAU, 0 kg N ha−1), 25% of the Abuja declaration (ADS 12.5 kg N ha−1), 50% of the Abuja declaration (ADS 25 kg N ha−1), and Abuja declaration–Abuja scenario (ADS, 50 kg N ha−1). The results revealed that increasing N input levels significantly influenced the growth and yield of maize crops. The ADS scenario recorded the highest grain yield increase (167.39%) in Nyando and 103.25% in Rangwe catchments compared to the BAU scenario. N deficits were observed in all the N use scenarios with a range of −66.6 to −125.7 kg N ha−1 in Nyando and −62.5 to −105.4 kg N ha−1 in Rangwe catchments with the 50% ADS scenario having the highest deficits. The deficits imply that the added N input is insufficient to create an N balance for optimal NUE with consequent high risks of soil N mining. In both catchments, all N use scenarios were within the recommended agro-physiological N efficiency (APEN) level of between 40 and 60 kg kg−1 N. The partial N balance obtained at Nyando (1.56–3.11) and Rangwe (1.10–4.64) was higher than the optimal values, a sign of insufficiency of N inputs and possible risk of soil N depletion in all the scenarios. Our findings conclude that the proposed N rates in the region are still very low for food sufficiency and optimized NUE. Therefore, there is a need to explore other sources of N such as biological N fixation and organic manure and inform policy- and decision-makers to recommend higher rates beyond the “Abuja declaration” with the prospect of reaching target yield and optimizing NUE values based on specific crop recommendations.
A Preliminary Study on the Resolution of Electro-Thermal Multi-Physics Coupling Problem Using Physics-Informed Neural Network (PINN)
Authors: Ma Yaoyao , Xu Xiaoyu , Yan Shuai , Ren Zhuoxiang
Published: February 2022

The problem of electro-thermal coupling is widely present in the integrated circuit (IC). The accuracy and efficiency of traditional solution methods, such as the finite element method (FEM), are tightly related to the quality and density of mesh construction. Recently, PINN (physics-informed neural network) was proposed as a method for solving differential equations. This method is mesh free and generalizes the process of solving PDEs regardless of the equations’ structure. Therefore, an experiment is conducted to explore the feasibility of PINN in solving electro-thermal coupling problems, which include the electrokinetic field and steady-state thermal field. We utilize two neural networks in the form of sequential training to approximate the electric field and the thermal field, respectively. The experimental results show that PINN provides good accuracy in solving electro-thermal coupling problems.
Applications of Magnetorheological Fluid Actuator to Multi-DOF Systems: State-of-the-Art from 2015 to 2021
Authors: Oh Jong-Seok , Sohn Jung Woo , Choi Seung-Bok
Published: February 2022

This review article presents various multi-DOF application systems that utilize smart magnetorheological (MR) fluid. It is well known that MR fluid has been actively studied and applied in many practical systems such as vehicle suspension dampers. The design requirements for the effective applications of MR fluid include geometry optimization, working principles, and control schemes. The geometry optimization is mostly related to the size minimization with high damping force, while the working principles are classified into the shear mode, the flow mode, and the squeeze mode depending on the dominant dynamic motion of the application system. The control schemes are crucial to achieve final targets such as robust vibration control against disturbances. It should be addressed that advanced output performances of MR application systems heavily depends on these three requirements. This review article presents numerous application systems such as sandwich structures, dampers, mounts, brakes, and clutches, which have been developed considering the three design requirements. In addition, in this article some merits and demerits of each application system are discussed to enable potential researchers to develop more effective and practical MR application systems featuring the multi-DOF dynamic motions.
Inhibitory Effects of Blue Light-Emitting Diode Irradiation on Podosphaera xanthii Conidial Release and Infection of Melon Seedlings
Authors: Suzuki Tomoko , Iwasaki Shota , Hisazumi Hatsune , Miyamoto Ayumi , Ogami Hayato , Takikawa Yoshihiro , Kakutani Koji , Matsuda Yoshinori , Nonomura Teruo
Published: February 2022

Powdery mildew fungi infect plant leaves, reducing the yield of infected melon plants. Therefore, an eco-friendly method of controlling powdery mildew in melon plants needs to be developed. A previous study described how the morphological characteristics of the conidiophores of the melon powdery mildew fungus Podosphaera xanthii Pollacci (designated KMP-6N) grown under greenhouse (natural) conditions and red light-emitting diode (LED) irradiation differed from those grown under growth chamber conditions and blue LED irradiation. In the present study, conidiophores with unconstricted conidia under blue light were collected and inoculated onto host leaves through micromanipulation; the unconstricted conidia germinated and infected the leaves, producing vigorously elongated hyphae. The number of conidia collected, the initial times of conidial release from single colonies, and the number of conidia remaining in chains on conidiophores were examined with electrostatic techniques. Under red light, the number of collected conidia gradually increased with the light irradiation period. The initial conidial release occurred between 2 to 4 h; the number of conidia remaining on the conidiophores gradually decreased and, eventually, the conidiophore lengths became shorter. In contrast, under blue light, few conidia were collected at any given time; the number of conidia on the conidiophores gradually increased and, eventually, the conidiophore lengths became longer. Next, the effects of red and blue light on the spread of powdery mildew infection by placing a KMP-6N-infected melon seedling at the centre of a tray containing healthy melon seedlings were examined. Almost all healthy seedlings caused powdery mildew symptoms at ca. 21 days after red light irradiation, whereas only healthy seedlings near the infected seedlings showed symptoms after blue light irradiation. Thus, the spread of melon powdery mildew infection clearly differed between red and blue light irradiation. This is the first report describing the effects of red and blue light on the spread of P. xanthii infection from a single infected seedling to healthy host seedlings; their results provide insight into the ecological mechanisms of powdery mildew conidial scatter from conidiophores.
Root System Architecture Differences of Maize Cultivars Affect Yield and Nitrogen Accumulation in Southwest China
Authors: Guo Song , Liu Zhigang , Zhou Zijun , Lu Tingqi , Chen Shanghong , He Mingjiang , Zeng Xiangzhong , Chen Kun , Yu Hua , Shangguan Yuxian , Dong Yujiao , Chen Fanjun , Liu Yonghong , Qin Yusheng
Published: February 2022

Root system architecture (RSA) plays a critical role in the acquisition of water and mineral nutrients. In order to understand the root characteristics that contribute to enhanced crop yield and N accumulation high-yielding and N efficient cultivars under N-stressed conditions. Here, grain yield, N accumulation and RSA traits of six dominant maize cultivars (CD30, ZH311, ZHg505, CD189, QY9 and RY1210) grown in the Southwestern part of China were investigated in field experiment under three different N regimes in 2019–2020; N300 (300 kg N ha−1), N150 (150 kg N ha−1) and N0 (no N supplied). Using Root Estimator for Shovelomics Traits (REST) for the quantitative analysis of maize root image obtained in the field, RSA traits including total root length (RL), root surface area (RA), root angle opening (RO), and root maximal width (RMW) were quantified in this study. The results showed that Yield, N accumulation and RSA were significantly affected by N rates, cultivars and their interactions. Grain yield, N accumulation and root weight showed a similar trend under N300 and N150 conditions compared to N0 conditions. With the input of N fertilizer, the root length, surface area, and angle increase, but root width does not increase. Under the N300 and N150 condition, RL, RA, RO and RMW increased by 17.96%, 17.74%, 18.27%, 9.22%, and 20.39%, 18.58%, 19.92%, 16.79%, respectively, compared to N0 condition. CD30, ZH505 and RY1210 have similar RO and RMW, larger than other cultivars. However, ZH505 and RY1210 have 13.22% and 19.99% longer RL, and 11.41% and 5.17% larger RA than CD30. Additionally, the grain yield of ZH505 and RY1210 is 17.57% and 13.97% higher compared with CD30. The N accumulation of ZH505 and RY1210 also shows 4.55% and 9.60% higher than CD30. Correlation analysis shows that RL, RA, RO and RMW have a significant positive correlation with grain yield while RO and RMW have a significant positive correlation with N accumulation. Linear plus plateau model analysis revealed that when the RO reaches 99.53°, and the RMW reaches 15.18 cm, the N accumulation reaches its maximum value under 0–300 kg N ha−1 conditions. Therefore, selecting maize cultivars with efficient RSA suitable for different soil N inputs can achieve higher grain yield and N use efficiency.
How Cover Crop Sowing Date Impacts upon Their Growth, Nutrient Assimilation and the Yield of the Subsequent Commercial Crop
Authors: Cottney Paul , Black Lisa , Williams Paul , White Ethel
Published: February 2022

Cover crops are typically sown post-harvest of commercial crops, prior to winter, which means that as sowing date is delayed, so will biomass production potential. The wide range of benefits associated with cover crops relies on them to produce sufficient biomass. Therefore, it must be identified how late certain species of cover crops can be sown. In the climatic conditions of Northern Ireland, not only has no research been conducted on how cover crops perform at various sowing dates but also their effect on the subsequent commercial crop yield has not been investigated. Addressing these issue will in turn help provide recommendations to maximise and encourage later sowing of cover crops. Consequently, five species of cover crops were chosen, from a range of families, then sown on 14 August, 7 September and 27 September. This is to mimic when land becomes fallow post-harvest of typical crops/rotations to this region. It was found that tillage radish (Raphanus sativus L.), when sown on the earliest date, could accumulate a maximum of 261 kg/ha of nitrogen (N), whereas, when sown on the last date, phacelia (Phacelia tanacetifolia L.) significantly outperformed all other species and assimilated 70 kg/ha of N. The cover crops were then incorporated into the soil and over-sown with spring barley (Hordeum vulgare L.). However, the spring barley yield was unaffected by any treatments. This trial shows that the non-leguminous species chosen are highly effective in assimilating nutrients when sown mid-August until early-September.
Preference of Pollinators over Various Forage Mixtures and Microelement Treatments
Authors: Hýbl Marian , Šipoš Jan , Krejčová Anna , Sodomová Karolína , Polák Ondřej , Koláčková Ivana , Mráz Petr
Published: February 2022

Low plant species richness and abundance, as well as contamination, can lead to a lack of nutrients in the diets of pollinators. This can cause increased oxidative stress, low resistance to disease and reduced detoxification ability. We proposed three forage mixtures with different botanical compositions, all of which should provide continuous forage for a wide range of pollinators. The monitored mixtures were treated by foliar application with selenium and zinc. Both elements should increase the quality of pollen and nectar. The effect of meadow mixtures and microelements on pollinator abundance, richness, and preference was evaluated using data obtained via the line transect method. Furthermore, the amount of selenium and zinc in the flowers was determined. It was found that the microelement treatment did not have any effect on the abundance and richness of the main pollinator taxa, which were affected only by a botanical composition of meadow mixtures. However, a preference for zinc-treated forage was observed in Apis mellifera, and a preference for selenium-treated forage was observed in Bombus spp. These two eusocial taxa appear to respond to an increased amount of microelements in the treated meadow mixtures. On the contrary, Lepidoptera was tied to untreated forage, where they were probably pushed by social bee species due to the competition.
Drug Repurposing Targeting Pseudomonas aeruginosa MvfR Using Docking, Virtual Screening, Molecular Dynamics, and Free-Energy Calculations
Authors: Vieira Tatiana F. , Magalhães Rita P. , Simões Manuel , Sousa Sérgio F.
Published: February 2022

Pseudomonas aeruginosa is an opportunistic Gram-negative bacterium responsible for acute and chronic infections in planktonic state or in biofilms. The sessile structures are known to confer physical stability, increase virulence, and work as a protective armor against antimicrobial compounds. P. aeruginosa can control the expression of genes, population density, and biofilm formation through a process called quorum sensing (QS), a rather complex and hierarchical system of communication. A recent strategy to try and overcome bacterial resistance is to target QS proteins. In this study, a combined multi-level computational approach was applied to find possible inhibitors against P. aeruginosa QS regulator protein MvfR, also known as PqsR, using a database of approved FDA drugs, as a repurposing strategy. Fifteen compounds were identified as highly promising putative MvfR inhibitors. On those 15 MvfR ligand complexes, molecular dynamic simulations and MM/GBSA free-energy calculations were performed to confirm the docking predictions and elucidate on the mode of interaction. Ultimately, the five compounds that presented better binding free energies of association than the reference molecules (a known antagonist, M64 and a natural inducer, 2-nonyl-4-hydroxyquinoline) were highlighted as very promising MvfR inhibitors.
Assessment of In Vitro Cefiderocol Susceptibility and Comparators against an Epidemiologically Diverse Collection of Acinetobacter baumannii Clinical Isolates
Authors: Ballesté-Delpierre Clara , Ramírez Ángel , Muñoz Laura , Longshaw Christopher , Roca Ignasi , Vila Jordi
Published: February 2022

Cefiderocol is a catechol-substituted siderophore cephalosporin combining rapid penetration into the periplasmic space with increased stability against β-lactamases. This study provides additional data on the in vitro antimicrobial activity of cefiderocol and commercially available comparators against an epidemiologically diverse collection of Acinetobacter baumannii clinical isolates. Antimicrobial susceptibility was tested using pre-prepared frozen 96-well microtiter plates containing twofold serial dilutions of: cefepime, ceftazidime/avibactam, imipenem/relebactam, ampicillin/sulbactam, meropenem, meropenem/vaborbactam, ciprofloxacin, minocycline, tigecycline, trimethoprim/sulfamethoxazole and colistin using the standard broth microdilution procedure in cation-adjusted Mueller–Hinton broth (CAMHB). For cefiderocol, iron-depleted CAMHB was used. A collection of 113 clinical strains of A. baumannii isolated from Argentina, Azerbaijan, Croatia, Greece, Italy, Morocco, Mozambique, Peru and Spain were included. The most active antimicrobial agents against our collection were colistin and cefiderocol, with 12.38% and 21.23% of non-susceptibility, respectively. A high proportion of multidrug-resistant (76.77%) and carbapenem-resistant (75.28%) A. baumannii isolates remained susceptible to cefiderocol, which was clearly superior to novel β-lactam/β-lactamase inhibitor combinations. Cefiderocol-resistance was higher among carbapenem-resistant isolates and isolates belonging to ST2, but could not be associated with any particular resistance mechanism or clonal lineage. Our data suggest that cefiderocol is a good alternative to treat infections caused by MDR A. baumanni, including carbapenem-resistant strains.
Insamgobonhwan Protects Neuronal Cells from Lipid ROS and Improves Deficient Cognitive Function
Authors: Yang Ji Hye , Nguyen Cong Duc , Lee Gihyun , Na Chang-Su
Published: February 2022

Iron is an essential element in the central nervous system that is involved in many of its important biological processes, such as oxygen transportation, myelin production, and neurotransmitter synthesis. Previous studies have observed the selective accumulation of iron in Aβ aggregates and neurofibrillary tangles in the brains of patients with Alzheimer’s disease, and excess of this accumulation is associated with accelerated cognitive decline in Alzheimer’s patients. Emerging evidence suggests that ferroptosis, cell death due to iron accumulation, is a potential therapeutic target for treating Alzheimer’s disease. Insamgobonhwan (GBH) is a well-regarded traditional medicine from Donguibogam that possess antioxidant properties and has been suggested to slow the aging process. However, the neuroprotective role of GBH against lipid peroxidation-induced ferroptosis and its positive cognitive effects remain unexplored. Here, we investigated the ability of GBH to protect against RSL3-induced ferroptosis in vitro and to suppress amyloid-β-induced cognitive impairment in vivo. First, we treated HT22 cells with RSL3 to induce ferroptosis, which is an inhibitor of glutathione peroxidase 4 (GPX4) and induces lethal lipid hydroperoxide accumulation, reactive oxygen species (ROS) production, and ferroptotic cell death. GBH treatment inhibited cell death and lipid peroxidation, which were increased by RSL3 administration. In addition, GBH restored the expression of ferroptosis marker proteins, such as GPX4, HO-1 and COX-2, which were altered by RSL3. Next, we examined whether the protective ability of GBH in cells was reproduced in animals. We concluded that GBH treatment inhibited Aβ-induced lipid peroxidation and improved Aβ-induced cognitive impairment in mice.
Neuroprotection against Aminochrome Neurotoxicity: Glutathione Transferase M2-2 and DT-Diaphorase
Authors: Segura-Aguilar Juan , Muñoz Patricia , Inzunza Jose , Varshney Mukesh , Nalvarte Ivan , Mannervik Bengt
Published: February 2022

Glutathione is an important antioxidant that plays a crucial role in the cellular protection against oxidative stress and detoxification of electrophilic mutagens, and carcinogens. Glutathione transferases are enzymes catalyzing glutathione-dependent reactions that lead to inactivation and conjugation of toxic compounds, processes followed by subsequent excretion of the detoxified products. Degeneration and loss of neuromelanin-containing dopaminergic neurons in the nigrostriatal neurons generally involves oxidative stress, neuroinflammation, alpha-synuclein aggregation to neurotoxic oligomers, mitochondrial dysfunction, protein degradation dysfunction, and endoplasmic reticulum stress. However, it is still unclear what triggers these neurodegenerative processes. It has been reported that aminochrome may elicit all of these mechanisms and, interestingly, aminochrome is formed inside neuromelanin-containing dopaminergic neurons during neuromelanin synthesis. Aminochrome is a neurotoxic ortho-quinone formed in neuromelanin synthesis. However, it seems paradoxical that the neurotoxin aminochrome is generated during neuromelanin synthesis, even though healthy seniors have these neurons intact when they die. The explanation of this paradox is the existence of protective tools against aminochrome neurotoxicity composed of the enzymes DT-diaphorase, expressed in these neurons, and glutathione transferase M2-2, expressed in astrocytes. Recently, it has been reported that dopaminergic neurons can be protected by glutathione transferase M2-2 from astrocytes, which secrete exosomes containing the protective enzyme.
Vulnerability of Triple-Negative Breast Cancer to Saponin Formosanin C-Induced Ferroptosis
Authors: Chen Hsin-Chih , Tang Han-Hsuan , Hsu Wei-Hsiang , Wu Shan-Ying , Cheng Wen-Hsing , Wang Bao-Yuan , Su Chun-Li
Published: February 2022

Targeting ferritin via autophagy (ferritinophagy) to induce ferroptosis, an iron- and reactive oxygen species (ROS)-dependent cell death, provides novel strategies for cancer therapy. Using a ferroptosis-specific inhibitor and iron chelator, the vulnerability of triple-negative breast cancer (TNBC) MDA-MB-231 cells to ferroptosis was identified and compared to that of luminal A MCF-7 cells. Saponin formosanin C (FC) was revealed as a potent ferroptosis inducer characterized by superior induction in cytosolic and lipid ROS formation as well as GPX4 depletion in MDA-MB-231 cells. The FC-induced ferroptosis was paralleled by downregulation of ferroportin and xCT expressions. Immunoprecipitation and electron microscopy demonstrated the involvement of ferritinophagy in FC-treated MDA-MB-231 cells. The association of FC with ferroptosis was strengthened by the results that observed an enriched pathway with differentially expressed genes from FC-treated cells. FC sensitized cisplatin-induced ferroptosis in MDA-MB-231 cells. Through integrated analysis of differentially expressed genes and pathways using the METABRIC patients’ database, we confirmed that autophagy and ferroptosis were discrepant between TNBC and luminal A and that TNBC was hypersensitive to ferroptosis. Our data suggest a therapeutic strategy by ferroptosis against TNBC, an aggressive subtype with a poor prognosis.
Brain Oxygen Perfusion and Oxidative Stress Biomarkers in Fetuses with Congenital Heart Disease—A Retrospective, Case-Control Pilot Study
Authors: Escobar-Diaz Maria C. , Pérez-Cruz Miriam , Arráez Miguel , Cascant-Vilaplana Mari-Merce , Albiach-Delgado Abel , Kuligowski Julia , Vento Máximo , Masoller Narcis , Gómez-Roig Maria Dolores , Gómez Olga , Sanchez-de-Toledo Joan , Camprubí-Camprubí Marta
Published: February 2022

Fetuses with congenital heart disease (CHD) have circulatory changes that may lead to predictable blood flow disturbances that may affect normal brain development. Hypoxemia and hypoperfusion may alter the redox balance leading to oxidative stress (OS), that can be assessed measuring stable end-products. OS biomarkers (OSB) were measured in amniotic fluid in fetuses with (n = 41) and without CHD (n = 44) and analyzed according to aortic flow, expected cyanosis after birth, and a CHD classification derived from this. Birth head circumference (HC) was used as a neurodevelopment biomarker. CHD fetuses had higher levels of ortho-Tyrosine (o-Tyr) than controls (p = 0.0003). There were no differences in o-Tyr levels considering aortic flow obstruction (p = 0.617). Fetuses with expected extreme cyanosis presented the highest levels of o-Tyr (p = 0.003). Among groups of CHD, fetuses without aortic obstruction and extreme cyanosis had the highest levels of o-Tyr (p = 0.005). CHD patients had lower HC than controls (p = 0.023), without correlation with OSB. Patients with HC < 10th percentile, presented high levels of o-Tyr (p = 0.024). Fetuses with CHD showed increased OSB and lower HC when compared to controls, especially those with expected extreme cyanosis. Our results suggest that increased levels of OSB are more influenced by the effect of low oxygenation than by aortic flow obstruction. Future studies with larger sample size are needed to further investigate the role of OSB as an early predictor of neurodevelopmental problems in CHD survivors.