bluuebunny/crossref_metadata_2025 huggingface dataset:
Extralinguistic Consultation in English–Chinese Translation: A Study Drawing on Eye-Tracking and Screen-Recording Data
Authors: Cui Yixiao , Zheng Binghan
Published: June 2022

Both linguistic and extralinguistic consultations are essential in translation practice and have been commonly investigated as an integral topic in previous studies. However, since extralinguistic information is usually longer in extent and not specifically designed for a linguistic purpose, extralinguistic consultations involve different search strategies compared with linguistic consultations. Drawing on eye-tracking and screen-recording data, this study compares linguistic and extralinguistic consultations in terms of cognitive resources allocation and information processing patterns in English–Chinese translation. It also explores the differences among 17 language learners, 20 student translators, and 21 professional translators, and the effect of extralinguistic consultation on their translation quality. The findings are as follows: (1) all participants allocate more attention and lower cognitive load to extralinguistic consultations than to linguistic consultations; (2) participants’ translation experience levels and their attention allocated to extralinguistic consultation show an inverted U-shaped relationship; and (3) participants who consult extralinguistic information before drafting or devote more attention to extralinguistic consultation produce target texts with significantly higher scores.
Adoption of AI-Enabled Tools in Social Development Organizations in India: An Extension of UTAUT Model
Authors: Jain Ruchika , Garg Naval , Khera Shikha N.
Published: June 2022

Social development organizations increasingly employ artificial intelligence (AI)-enabled tools to help team members collaborate effectively and efficiently. These tools are used in various team management tasks and activities. Based on the unified theory of acceptance and use of technology (UTAUT), this study explores various factors influencing employees’ use of AI-enabled tools. The study extends the model in two ways: a) by evaluating the impact of these tools on the employees’ collaboration and b) by exploring the moderating role of AI aversion. Data were collected through an online survey of employees working with AI-enabled tools. The analysis of the research model was conducted using partial least squares (PLS), with a two-step model – measurement and structural models of assessment. The results revealed that the antecedent variables, such as effort expectancy, performance expectancy, social influence, and facilitating conditions, are positively associated with using AI-enabled tools, which have a positive relationship with collaboration. It also concluded a significant effect of AI aversion in the relationship between performance expectancy and use of technology. These findings imply that organizations should focus on building an environment to adopt AI-enabled tools while also addressing employees’ concerns about AI.
Bilingual Processing Mechanisms of Scientific Metaphors and Conventional Metaphors: Evidence via a Contrastive Event-Related Potentials Study
Authors: Tang Xuemei , Shen Lexian , Yang Peng , Huang Yanhong , Huang Shaojuan , Huang Min , Ren Wei
Published: June 2022

To study the different mechanisms of understanding figurative language in a speaker’s native language (L1) and their second language (L2), this study investigated how scientific metaphors in Chinese (L1) and English (L2) are electrophysiologically processedviaevent-related potential experimentation. Compared with the metaphors from daily life or in literary works, scientific metaphors tend to involve both a more complicated context structure and a distinct knowledge-inferencing process. During the N400 time window (300–500 ms), English scientific metaphors elicited more negative N400s than Chinese ones at the parietal region. In the late positive component (LPC) time window (550–800 ms), English scientific metaphors elicited less positive LPCs than Chinese ones at the parietal region, and larger late negativities encompassing smaller areas of the brain. The findings might indicate that for late unbalanced bilingual speakers, L2 scientific metaphor comprehension requires more effort in information retrieval or access to the non-literal route. Altogether, the possible findings are that non-native and non-dominant language processing involves decreased automaticity of cognitive mechanisms, and decreased sensitivity to the levels of conventionality of metaphoric meanings.
The Association Between Perceived Risk of COVID-19, Psychological Distress, and Internet Addiction in College Students: An Application of Stress Process Model
Authors: Chang Biru , Hou Jianhua
Published: June 2022

The closed-off management of the university during coronavirus disease 2019 (COVID-19) may be associated with an elevated odds of psychological and behavioral issues among college students. We aimed to use the stress-process model to explore the potential mechanisms for this phenomenon. A total of 924 college students were recruitedviaposters, peer referrals, and class attendance. Among them, 82 (9%) were probable depression, 190 (20.8%) were probable anxiety, and 69 (7.5%) were internet addiction. Parallel mediation was used to test this theoretical model. For personal resources, the perceived risk of COVID-19 was positively associated with psychological distressvianegative coping style (β = 0.051) and internet addictionvianegative coping style or self-esteem (β = 0.023 for negative coping style, β = 0.015 for self-esteem). For social resources, the perceived risk of COVID-19 was positively associated with psychological distress and internet addictionviaroommate relationships (β = 0.19 for psychological distress, β = 0.046 for internet addiction). Negative coping styles and roommate relationships are possible psychological mechanisms linking the perceived risk of COVID-19, psychological distress, and internet addiction.
Analysis of Educational Mental Health and Emotion Based on Deep Learning and Computational Intelligence Optimization
Authors: Liu Junli , Wang Haoyuan
Published: June 2022

Understanding students’ psychological pressure and bad emotional reaction can solve psychological problems as soon as possible and avoid affecting students’ normal study life. With the improvement of global scientific and technological strength, and the step-by-step in-depth research on deep learning and computational intelligence optimization. Now, we have enough conditions to build a psychological and emotional data set for the field of education, and build a mental health stress detection model with emotional analysis function. In addition, a variety of experimental methods are used for comparison, which shows the superior performance of the model in practical application scenarios. The results show that: (1) the data set constructed for the model is reasonable. Psychological stress test shows that the tested college students are in good health and have no positive performance. Schools need to pay special attention to obsessive–compulsive disorder and interpersonal sensitivity, and the average values of both indicators are higher than 0.9. (2) For the optimization of ant colony algorithm (ACO) computational intelligence, both the stability and the average execution time of the algorithm are obviously higher than those of other algorithms. This model has obvious performance advantages after using this algorithm. (3) Using loss function value to measure the difference between simulated emotion analysis and real value. The difference of most emotion tests is less than 3%; the accuracy difference between sadness and fear is about 7%. Although the final results prove the feasibility of this method, there are still some shortcomings to be optimized.
Women's Estrus and Extended Sexuality: Reflections on Empirical Patterns and Fundamental Theoretical Issues
Authors: Gangestad Steven W. , Dinh Tran
Published: June 2022

How do women's sexual interests change across their ovulatory cycles? This question is one of the most enduring within the human evolutionary behavioral sciences. Yet definitive, agreed-upon answers remain elusive. One empirical pattern appears to be robust: Women experience greater levels of sexual desire and interest when conceptive during their cycles. But this pattern is not straightforward or self-explanatory. We lay out multiple possible, broad explanations for it. Based on selectionist reasoning, we argue that the conditions that give rise to sexual interests during conceptive and non-conceptive phases are likely to differ. Because conceptive and non-conceptive sex have distinct functions, the sexual interests during conceptive and non-conceptive phases are likely to have different strategic ends. We discuss provisional evidence consistent with this perspective. But the exact nature of women's dual sexuality, if it exists, remains unclear. Additional empirical research is needed. But perhaps more crucially, this topic demands additional theory that fruitfully guides and interprets future empirical research.
Physician Assisted Death for Psychiatric Suffering: Experiences in the Netherlands
Authors: van Veen SMP , Widdershoven GAM , Beekman ATF , Evans N.
Published: June 2022

Physician assisted death (PAD) for patients with a psychiatric disorder is a controversial topic of increasing relevance, since a growing number of countries are allowing it. General requirements for PAD include that patients possess decision-making capacity to decide on PAD and that their suffering is unbearable and irremediable. In the Netherlands PAD has been eligible for patients with psychiatric disorders since the 1990s, making it one of the few countries that can offer insights on the practice from real life experience. Much of the literature describing these experiences is only available in Dutch. This article aims to make this knowledge more widely available and provide a comprehensive overview of the experience with PAD for psychiatric suffering in the Netherlands. First, the history of PAD for patients suffering from a psychiatric disorder is described. Second, an overview of relevant rules and regulations governing the practice is given. Third, an overview is provided of the scarce epidemiological data. Finally, we will discuss two major clinical challenges; establishing irremediability and decision-making capacity.
Practices and Policies Around Wellness: Insights From the Internet Crimes Against Children Task Force Network
Authors: Mitchell Kimberly J. , Gewirtz-Meydan Ateret , O'Brien Jennifer , Finkelhor David
Published: June 2022

This article aims to understand what practices and training Internet Crimes Against Children (ICAC) Task Forces and affiliated agencies are currently using to help mitigate distress and promote wellbeing among investigators of child sexual abuse material (CSAM). Data were collected via telephone interviews with Commanders of 54 ICAC Task Forces as well as an online survey of 155 investigators at ICAC-affiliated agencies. Sixty-two percent of respondents said their agency had an Officer Wellness Program. Findings highlighted considerable gaps in protective practices with 46.1% of respondents mentioning the need for more wellness resources in their agency for personnel who have viewed CSAM as a high priority. Stigma created by help-seeking was the most widely acknowledged barrier discussed in relation to police wellness. A large and salient problem was the persistent lack of wellness practices in the affiliated agencies in comparison to the Task Forces themselves. Exposure to CSAM can be a source of stress for personnel and the results indicate concern about the problem and a diffusion of proactive initiatives, but barriers and inconsistent adoption remain.
Assessment of Willingness to Pay for Pollution Prevention, Health and Happiness: A Case Study of Punjab, Pakistan
Authors: Rafique Muhammad Zahid , Sun Jinping , Larik Abdul Razaque , Li Yafei
Published: June 2022

Air pollution has been notoriously held accountable for a substantial number of deaths in several countries. Moreover, its negative impact on people's health and well-being has also been witnessed in countries where air pollution is below the recommended national levels. The urban cities of Pakistan are among the worst South Asian areas in terms of air pollution. Because of this problem, the health and well-being of citizens are affected. The present study investigates the impact of air pollution on urban residents' happiness and health. It analyzes their willingness to pay for pollution prevention and its determinants by employing the data obtained through a primary survey. Pakistanis are unaware of air pollution's effect on health and quality of life, therefore only 12.5% consider this problem very serious. The results confirm the significantly negative effect of air pollution on happiness. Concerning the willingness to pay, it is differentiated in the form of tax and social contribution. Pakistanis are willing to pay more in social contribution in return for different environmental attributes. The results show that only 13% of respondents are not willing to pay for income contribution to improve air quality reporting indifferent attitude and insufficient knowledge of the environment. Our findings suggest that their apprehension concerning the environment influences people's willingness to pay. The study concludes that despite Pakistan's underdeveloped economic stature and its poor and flexible budgetary allocation for the betterment of air quality, most Pakistanis showed their willingness to pay for environmental protection. The government and environmental organizations ought to generate consensus among the general population about environmental importance, individual responsibility, and social duties thereby lessening the free-rider problem and reducing air pollution for better social welfare.
Wearable Walking Assistant for Freezing of Gait With Environmental IoT Monitoring: A Contribution to the Discussion
Authors: Bernardes Rafael A. , Ventura Filipa , Neves Hugo , Fernandes Maria Isabel , Sousa Pedro
Published: June 2022

Parkinson's disease (PD) is the second most common neurodegenerative disease, significantly increasing in the last three decades. Worldwide, seven to ten million people are affected by PD. In people living with PD, freezing of gait (FoG) significantly impacts activities of daily living, potentially leading to falls, injuries, and loss of autonomy. FoG prevalence rates vary widely, reaching at least 50% of patients with PD. Current therapeutic options have limited effectiveness, and their complement with innovative technology-based solutions in the real world is demanded to enhance daily functioning for people living with PD. This article provides a narrative review of current technological developments for people living with PD and, derived from that evidence, presents a perspective on integrating wearable technology and IoT to support telemonitoring and self-management of people living with PD in their daily living environment. Complementing current therapeutic options with technology-based solutions in PD patients' real-world environment is crucial to enhancing the quality of life of people living with PD. In that way, wearable technology and IoT might constitute resources of excellence in seamless monitoring and self-management in people's home environments.
Knowledge, Attitude, Practices, and Sources of Information (KAPS) Toward COVID-19 During the Second Wave Pandemic Among University Population in Qatar: A Cross-Sectional Study
Authors: Alkaabi Ibrahim , Abita Magdy , Mahdi Yousif , Ouda Amr , Malki Mohammed Imad
Published: June 2022

Background

The World Health Organization (WHO) declared COVID-19 as a pandemic on 11 March 2020. Many efforts were performed to contain the virus worldwide. People's knowledge and attitude should be directed toward strict preventive practices to halt the spread of the virus. We aimed to assess the knowledge, attitude, practices, and sources of information (KAPS) used by Qatar University (QU) attendees.

Methods

A cross-sectional web-based questionnaire was answered by 500 employees and students in the QU community. It included questions on KAPS toward COVID-19. Information on sociodemographics was collected and analyzed. This study was conducted during the second wave of COVID-18 in the state of Qatar (April–May 2021).

Results

A total of 475 participants aged between 18 and 68 years old consented to complete the survey questionnaire. The study involved 279 (58.7%) non-Qatari nationals and 196 (41.3%) natives, with 254 (53.5%) participants pursuing postgraduate studies and 221 (46.5%) undergraduates. Approximately two-thirds of the sample were employed (64.8%), while one-third were unemployed (35.2%). Knowledge scores on average were 66.4% (M = 5.31, SD = 1.45, and range: 0–8), with only significant differences were noted between nationalities (natives and non-natives) Participants' average score in practices was 69.72% (M = 4.18, SD = 1.7, and range 0–6) with a significant difference in safe COVID-19 practice scores based on the educational level. Adherence with COVID-19 policies and rules were 82% (M = 2.46, SD = 0.7, and range: 0–3) with no differences noted between groups. In addition, the population reported relying on governmental press conferences (76.0%) as their primary source of gaining details concerning COVID-19, followed by social media (64.4%). The least popular resources were information gained from family, relatives, friends, and coworkers (47.4%) and the news channels on TV (46.7%).

Conclusion

Overall, this study provides insights into Qatar's KAPS toward COVID-19 during the quarantine of the second wave of this pandemic. This study, being the first of its kind to be conducted in the state of Qatar, is expected to help the ministry of public health and the government communication office to establish a suitable measurement of response to the spread of COVID-19 and develop the best practices for any future epidemics that might occur.
In vitro Effect of Geranylgeraniol (GGOH) on Bisphosphonate-Induced Cytotoxicity of Oral Mucosa Cells
Authors: Rattanawonsakul Krit , Bullock George , Bolt Robert , Claeyssens Frederik , Atkins Simon , Hearnden Vanessa
Published: June 2022

Medication-related osteonecrosis of the jaw (MRONJ) is an often-severe complication found in patients receiving bisphosphonates in the management of Paget's, osteoporosis and metastatic bone cancer. Mucosal breakdown with bone exposure is a primary clinical presentation of MRONJ linked to the inhibitory effect of nitrogen-containing bisphosphonates (N-BP) on the mevalonate pathway. Geranylgeraniol (GGOH) has demonstrated a rescue effect on N-BP-treated osteoclasts but the biological effects on oral soft tissues and cells remain unclear. This study aimed to determine whether GGOH could prevent bisphosphonate induced toxicity to oral mucosa cells in vitro. Primary oral fibroblasts and keratinocytes were exposed to different GGOH concentrations or GGOH in combination with two nitrogen-containing bisphosphonates, zoledronic acid (ZA) or pamidronic acid (PA), for 72 h. The metabolic activity of each cell type was measured using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. GGOH without bisphosphonates significantly reduced the metabolic activity of oral mucosa cells. Fibroblasts treated with GGOH and ZA in combination showed a slight increase in metabolic status compared to fibroblasts treated with ZA alone, however this positive effect was not observed in keratinocytes. In the presence of PA, GGOH was unable to increase the metabolic activity of either cell type. These findings demonstrate that GGOH is toxic to oral mucosa cells and that GGOH was not able to prevent bisphosphonate induced toxicity. These data show that GGOH does not have therapeutic potential for bisphosphonate-induced soft tissue toxicity in MRONJ and the use of GGOH as an MRONJ treatment should be strongly reconsidered.
Effectiveness and Validation of the Italian Translation of the Low Anterior Resection Syndrome Score in an Italian High-Volume University Hospital
Authors: De Simone Veronica , Litta Francesco , Persiani Roberto , Rizzo Gianluca , Sofo Luigi , Menghi Roberta , Santullo Francesco , Biondi Alberto , Coco Claudio , Sacchetti Franco , Longo Fabio , Attalla El Halabieh Miriam , Moroni Rossana , Ratto Carlo
Published: June 2022

Background

The low anterior resection syndrome (LARS) score is a validated questionnaire developed in Denmark to measure the severity of bowel dysfunction after low anterior resection. This retrospective study aimed to assess the effectiveness of the LARS score in the Italian language in a population of Italian patients who underwent low anterior resection for rectal cancer. The convergent and discriminative validity and the test-retest reliability of the score were investigated.

Methods

A cohort of two hundred and five patients treated with low anterior resection were enrolled in an Italian high-volume university hospital between January 2000 and April 2018. The Italian version of the LARS score (tested twice), as translated from English original version, a single question on quality of life and the EORTC QLQ-C30 questionnaire were submitted to patients.

Results

A high proportion of patients showed a perfect or moderate fit between the LARS score and QoL categories (convergent validity, p < 0.0005). All differences regarding the items of the European Organization for Research and Treatment of Cancer Quality of Life Questionnaire – Core 30 (EORTC QLQ-C30) functional scales were statistically significant (p < 0.0005). The LARS score was able to discriminate between groups of patients who received or did not receive preoperative chemoradiotherapy (p < 0.0005) and those who received total or partial mesorectal excision (p < 0.0005). The test-retest reliability was excellent (intraclass correlation coefficient 0.96).

Conclusion

The Italian translation of the LARS score is an easy and reliable tool for assessing bowel dysfunction after low anterior resection and its routine use in clinical practice should be recommended.

Trial registration number at www.clinicaltrials.gov: NCT04406311.
Fluorescence Biomodulation for Canine Interdigital Furunculosis: Updates for Once-Weekly Schedule
Authors: Marchegiani Andrea , Fruganti Alessandro , Gavazza Alessandra , Spaterna Andrea , Cerquetella Matteo
Published: June 2022

Interdigital furunculosis is a common multifactorial, inflammatory disease of the canine interdigital skin in which lesions commonly become secondarily infected. Fluorescence biomodulation (FBM) administered twice weekly has shown to effectively control clinical manifestation as adjunct therapy to systemic antibiotic. Since twice weekly regimen could be unaffordable for some pet owners, the aim of this study was to evaluate the effect of once weekly application of FBM in combination with systemic antibiotic on clinical manifestations of canine interdigital pyoderma, comparing the results to those present in literature. Twelve dogs diagnosed with interdigital pyoderma received antibiotic plus once weekly FBM application. Dogs were scored until complete healing based on global lesion score and neutrophil engulfing bacterial score. The results obtained demonstrated that once weekly application of FBM exerts the same beneficial effect on interdigital furunculosis healing as per twice weekly, indicating that once weekly regimen is well tolerated and is yielding similar results to twice weekly applications.
The Probiotic Attributes and Anti-pseudorabies Virus Evaluation of Lactobacillus Isolates
Authors: Yang Ming-Fan , Yan Wei , Li Yan , Li Shuai-Qi , Chen Hong-Ying , Yin Qing-Qiang , Dang Xiao-Wei , Zhang Hong-Ying
Published: June 2022

The emergence of pseudorabies virus (PRV) variants brings serious harm to the swine industry, and its effective treatments are limited at present. As one of the probiotics, the Lactobacillus species have beneficial characteristics of regulating the balance of intestinal flora, inhibiting the growth of pathogenic bacteria and viruses' proliferation, and improving self-immunity. In this study, Lactobacillus plantarum HN-11 and Lactobacillus casei HN-12 were selected and identified through morphology observation, Gram stain microscopy, 16S rRNA sequencing analysis, and specific amplification of the recA gene and pheS gene. All tested isolates exhibited rapid adaptation to the different conditions, excellent acid, and bile tolerance, and sensitivity to Salmonella, Staphylococcus aureus, and Escherichia coli. The antibiotic susceptibility assay displayed the isolates sensitive to most antibiotics and resistant to Lincomycin and Norfloxacin. Moreover, the supernatants of HN-11 and HN-12 inhibited PRV proliferation in ST cells. The results of animal experiments showed that supplementing the challenged mice with the supernatants of Lactobacillus isolates in advance delayed the course of the disease. PRV was detected in the heart, liver, spleen, lung, kidney, and brain tissues of dead mice in the test groups, and its copies in the lungs were significantly decreased compared with the control mice (P < 0.05). These findings proved the advantages of L. plantarum and L. casei as potential probiotic cultures, which could provide a basis for its application in microecological preparations and functional formulations.
Is Avoidable Hospitalization Experienced Prior to Infection Associated With COVID-19-Related Deaths?
Authors: Lee Woo-Ri , Yoo Ki-Bong , Lee Gyeong-Min , Koo Jun Hyuk , Kim Li-Hyun
Published: June 2022

Objectives: This study aimed to determine the effect of the presence or absence of avoidable hospitalization before acquiring coronavirus disease (COVID-19) on COVID-19-related deaths.

Methods: This study used the total NHIS-COVID-19 dataset comprising domestic COVID-19 patients, provided by the National Health Insurance Service (NHIS) in South Korea. We conducted logistic regression and double robust estimation (DRE) to confirm the effect of avoidable hospitalization on COVID-19-related deaths.

Results: Logistic regression analysis confirmed that the odds ratio (OR) of death due to COVID-19 was high in the group that experienced avoidable hospitalization. DRE analysis showed a higher OR of death due to COVID-19 in the group that experienced avoidable hospitalization compared to the group that did not experience avoidable hospitalization, except in the subgroup aged ≤69 years.

Conclusion: The effect of avoidable hospitalization on COVID-19-related deaths was confirmed. Therefore, continued health care, preventive medicine, and public health management are essential for reducing avoidable hospitalizations despite the COVID-19 pandemic. Clinicians need to be informed about the importance of continuous disease management.
Analysis of the Effect of Tumor-Grade Change on the Prognosis of Retroperitoneal Sarcoma
Authors: Jo Sung Jun , Kim Kyeong Deok , Lim So Hee , Kim Jinseob , Kim Min Jung , Park Jae Berm , Lee Kyo Won
Published: June 2022

In retroperitoneal sarcoma (RPS), the change in the tumor grade from the primary tumor to the first local recurrence, and the effect of this change on prognosis, are unknown. The aim of this study is to analyze whether these changes affect the prognosis of RPS. Patients who underwent surgery for a first locally recurrent RPS at Samsung Medical Center from January 2001 to February 2020 were included. The pathologic features of primary and recurrent tumors were compared, and the outcomes were measured. A total of 49 patients were investigated. There were 25 patients with different grades of primary and recurrent tumors. The improving, stable, and worsening groups contained 16 (32.7%), 24 (49%), and 9 (18.3%) patients, respectively. There was no significant difference in the prognosis between the three groups. In the analyses of the factors that affect the OS, a high grade of the primary tumor (p = 0.023) and the size of the recurrent tumor (p = 0.032) were statistically significant in both univariate and multivariate analyses. In a factor analysis of the second LR, a high-grade recurrent tumor (p = 0.032) was the only significant factor. There were tumor-grade changes between the primary tumor and recurrent tumor in RPS. However, the most important factor in prognosis is a high grade of the primary tumor.
Potential Pro-Tumorigenic Effect of Bisphenol A in Breast Cancer via Altering the Tumor Microenvironment
Authors: Kwon Youngjoo
Published: June 2022

BPA, a chemical used in the preparation of polycarbonate plastics, is an endocrine disruptor. Exposure to BPA has been suggested to be a risk factor for breast cancer because of its potential to induce estrogen receptor signaling in breast cancer cells. More recently, it has been recognized that BPA also binds to the G protein-coupled estrogen receptor and other nuclear receptors, in addition to estrogen receptors, and acts on immune cells, adipocytes, and fibroblasts, potentially modulating the TME. The TME significantly impacts the behavior of cancer cells. Therefore, understanding how BPA affects stromal components in breast cancer is imperative to adequately assess the association between exposure to BPA and the risk of breast cancer. This review examines the effects of BPA on stromal components of tumors to highlight their potential role in the carcinogenic effect of BPA. As a result, I propose considerations for the risk assessment of BPA exposure and studies needed to improve understanding of the TME-mediated, breast cancer-promoting effect of BPA.
A Mathematical Epidemiological Model (SEQIJRDS) to Recommend Public Health Interventions Related to COVID-19 in Sri Lanka
Authors: Wijesekara Patikiri Arachchige Don Shehan Nilmantha , Wang Yu-Kai
Published: June 2022

Coronavirus disease 2019 (COVID-19) has been causing negative impacts on various sectors in Sri Lanka, as a result of the public health interventions that the government had to implement in order to reduce the spread of the disease. Equivalent work carried out in this context is outdated and close to ideal models. This paper presents a mathematical epidemiological model, called SEQIJRDS, having additional compartments for quarantine and infected people divided into two compartments as diagnosed and non diagnosed, compared to the SEIR model. We have presented the rate equations for the model and the basic reproduction number is derived. This model considers the effect of vaccination, the viral load of the variants, mask use, mobility, contact tracing and quarantine, natural immunity development of the infected people, and immunity waning of the recovered group as key developments of the model. The model has been validated for the COVID-19 pandemic in Sri Lanka by parameter derivation using mathematical formulations with the help of the existing data, the literature, and by model fitting for historical data. We present a comparison of the model projections for hospitalized infected people, the cumulative death count, and the daily death count against the ground truth values and projections of the SEIR and SIR models during the model validation. The validation results show that the proposed SEQIJRDS model’s 12-week projection performance is significantly better than both the SEIR and SIR models; the 2-, 6-, 8-, and 10-week projection performance is always better, and the 4-week projection performance is only slightly inferior to other models. Using the proposed SEQIJRDS model, we project mortality under different lockdown procedures, vaccination procedures, quarantine practices, and different mask-use cases. We further project hospital resource usage to understand the best intervention that does not exhaust hospital resources. At the end, based on an understanding of the effect of individual interventions, this work recommends combined public health interventions based on the projections of the proposed model. Specifically, three recommendations—called minimum, sub-optimum, and optimum recommendations—are provided for public health interventions.
Styryl Hemicyanine Dye (E)-3-Methyl-2-(4-thiomorpholinostyryl)benzo[d]thiazol-3-ium Iodide for Nucleic Acids and Cell Nucleoli Visualization
Authors: Vasilev Aleksey A. , Miteva Marina , Ishkitiev Nikolay , Dragneva Maria , Topalova Lora , Kandinska Meglena I.
Published: June 2022

(E)-3-Methyl-2-(4-thiomorpholinostyryl)benzo[d]thiazol-3-ium iodide 1 was prepared by a convenient and reliable reaction procedure. The slight molar excess of the starting benzaldehyde and the mixture of ethanol: ethyl acetate in the ratio 3:1 as a solvent afforded a pure reaction product. The photophysical properties of the dye in a TE buffer in the absence and presence of double-stranded DNA (dsDNA) were elucidated. The low intrinsic fluorescence of 1 in TE buffer is followed by an increase in the fluorescence after dsDNA binding. The dye is nontoxic for stem cells from apical papilla and the most concentrated fluorescence is detected in the cell nucleoli.
Synthesis and Antileukemia Activity Evaluation of Benzophenanthridine Alkaloid Derivatives
Authors: Tang Yaling , Xu Xinglian , Li Jiang , Deng Lulu , Mu Shuzhen
Published: June 2022

Thirty-three benzophenanthridine alkaloid derivatives (1a–1u and 2a–2l) were synthesized, and their cytotoxic activities against two leukemia cell lines (Jurkat Clone E6-1 and THP-1) were evaluated in vitro using a Cell Counting Kit-8 (CCK-8) assay. Nine of these derivatives (1i–l, 2a, and 2i–l) with IC50 values in the range of 0.18–7.94 μM showed significant inhibitory effects on the proliferation of both cancer cell lines. Analysis of the primary structure–activity relationships revealed that different substituent groups at the C-6 position might have an effect on the antileukemia activity of the corresponding compounds. In addition, the groups at the C-7 and C-8 positions could influence the antileukemia activity. Among these compounds, 2j showed the strongest in vitro antiproliferative activity against Jurkat Clone E6-1 and THP-1 cells with good IC50 values (0.52 ± 0.03 μM and 0.48 ± 0.03 μM, respectively), slightly induced apoptosis, and arrested the cell-cycle, all of which suggests that compound 2j may represent a potentially useful start point to undergo further optimization toward a lead compound.
Multi-Dose Intravenous Administration of Neutral and Cationic Liposomes in Mice: An Extensive Toxicity Study
Authors: Andrade Stéphanie , Loureiro Joana A. , Ramirez Santiago , Catumbela Celso S. G. , Soto Claudio , Morales Rodrigo , Pereira Maria Carmo
Published: June 2022

Liposomes are widely used as delivery systems for therapeutic purposes. However, the toxicity associated with the multi-dose administration of these nanoparticles is not fully elucidated. Here, we evaluated the toxicity of the prolonged administration of liposomes composed of neutral or cationic phospholipids often used in drug and gene delivery. For that purpose, adult wild-type mice (C57Bl6) were randomly distributed into three groups receiving either vehicle (PBS), neutral, or cationic liposomes and subjected to repeated intravenous injections for a total of 10 doses administered over 3 weeks. Several parameters, including mortality, body weight, and glucose levels, were monitored throughout the trial. While these variables did not change in the group treated with neutral liposomes, the group treated with the positively charged liposomes displayed a mortality rate of 45% after 10 doses of administration. Additional urinalysis, blood tests, and behavioral assays to evaluate impairments of motor functions or lesions in major organs were also performed. The cationic group showed less forelimb peak force than the control group, alterations at the hematological level, and inflammatory components, unlike the neutral group. Overall, the results demonstrate that cationic liposomes are toxic for multi-dose administration, while the neutral liposomes did not induce changes associated with toxicity. Therefore, our results support the use of the well-known neutral liposomes as safe drug shuttles, even when repetitive administrations are needed.
A Review on the Role of Pilocarpine on the Management of Xerostomia and the Importance of the Topical Administration Systems Development
Authors: Kapourani Afroditi , Kontogiannopoulos Konstantinos N. , Barmpalexis Panagiotis
Published: June 2022

Xerostomia is linked to an increased risk of dental caries, oral fungal infections, and speaking/swallowing difficulties, factors that may significantly degrade patients’ life, socially- or emotionally-wise. Consequently, there is an increasing interest in developing management approaches for confronting this oral condition, at which pilocarpine, a parasympathomimetic agent, plays a vital role. Although the therapeutic effects of orally administrated pilocarpine on the salivary gland flow and the symptoms of xerostomia have been proved by numerous studies, the systemic administration of this drug is affiliated with various adverse effects. Some of the typical adverse effects include sweating, nausea, vomiting, diarrhea, rhinitis, dizziness and increased urinary frequency. In this vein, new strategies to develop novel and effective dosage forms for topical (i.e., in the oral cavity) pilocarpine administration, in order for the salivary flow to be enhanced with minimal systemic manifestations, have emerged. Therefore, the purpose of the current review is to survey the literature concerning the performance of topical pilocarpine delivery systems. According to the findings, the topical delivery of pilocarpine can be regarded as the equivalent to systemic delivery of the drug, efficacy-wise, but with improved patient tolerance and less adverse effects.
Advanced Analytical Approach Based on Combination of FT-IR and Chemometrics for Quality Control of Pharmaceutical Preparations
Authors: Foschi Martina , Marziale Mariagrazia , Biancolillo Alessandra
Published: June 2022

Background: The present work represents a feasibility study for the realization of an analytical method finalized to the detection of expired antibiotic tablets. The work focuses on a specific antibiotic drug and represents the preliminary study upstream of a larger-scale work. Methods: attenuated Total reflection-Fourier transform infrared spectroscopy (ATR-FTIR) spectra coupled with sequential preprocessing through an orthogonalization (SPORT) chemometric approach were used to discriminate between expired and compliant tablets. Conclusions: The highest predictive accuracy (93.3% of correct classification rate in external validation, corresponding to 1 misclassified test sample over 15) was achieved by analyzing intact tablets. This represents an excellent result because it gives indications regarding the possibility of determining, in a completely non-destructive way, the presence of expired drugs.
Comparison of Physicochemical Properties of LipoParticles as mRNA Carrier Prepared by Automated Microfluidic System and Bulk Method
Authors: Ayad Camille , Yavuz Altan , Salvi Jean-Paul , Libeau Pierre , Exposito Jean-Yves , Ginet Valentine , Monge Claire , Verrier Bernard , Arruda Danielle Campiol
Published: June 2022

Polymeric and/or lipid platforms are promising tools for nucleic acid delivery into cells. We previously reported a lipid–polymer nanocarrier, named LipoParticles, consisting of polylactic acid nanoparticles surrounded by cationic lipids, and allowing the addition of mRNA and cationic LAH4-1 peptide at their surface. Although this mRNA platform has shown promising results in vitro in terms of mRNA delivery and translation, the bulk method used to prepare LipoParticles relies on a multistep and time-consuming procedure. Here, we developed an automated process using a microfluidic system to prepare LipoParticles, and we compared it to the bulk method in terms of morphology, physicochemical properties, and ability to vectorize and deliver mRNA in vitro. LipoParticles prepared by microfluidic presented a smaller size and more regular spherical shape than bulk method ones. In addition, we showed that the total lipid content in LipoParticles was dependent on the method of preparation, influencing their ability to complex mRNA. LipoParticles decorated with two mRNA/LAHA-L1 ratios (1/20, 1/5) could efficiently transfect mouse DC2.4 cells except for the automated 1/5 assay. Moreover, the 1/5 mRNA/LAHA-L1 ratio drastically reduced cell toxicity observed in 1/20 ratio assays. Altogether, this study showed that homogeneous LipoParticles can be produced by microfluidics, which represents a promising platform to transport functional mRNA into cells.
Freeze-Drying of a Capsid Virus-like Particle-Based Platform Allows Stable Storage of Vaccines at Ambient Temperature
Authors: Aves Kara-Lee , Janitzek Christoph M. , Fougeroux Cyrielle E. , Theander Thor G. , Sander Adam F.
Published: June 2022

The requirement of an undisrupted cold chain during vaccine distribution is a major economic and logistical challenge limiting global vaccine access. Modular, nanoparticle-based platforms are expected to play an increasingly important role in the development of the next-generation vaccines. However, as with most vaccines, they are dependent on the cold chain in order to maintain stability and efficacy. Therefore, there is a pressing need to develop thermostable formulations that can be stored at ambient temperature for extended periods without the loss of vaccine efficacy. Here, we investigate the compatibility of the Tag/Catcher AP205 capsid virus-like particle (cVLP) vaccine platform with the freeze-drying process. Tag/Catcher cVLPs can be freeze-dried under diverse buffer and excipient conditions while maintaining their original biophysical properties. Additionally, we show that for two model cVLP vaccines, including a clinically tested SARS-CoV-2 vaccine, freeze-drying results in a product that once reconstituted retains the structural integrity and immunogenicity of the original material, even following storage under accelerated heat stress conditions. Furthermore, the freeze-dried SARS-CoV-2 cVLP vaccine is stable for up to 6 months at ambient temperature. Our study offers a potential solution to overcome the current limitations associated with the cold chain and may help minimize the need for low-temperature storage.
Electric Field Sensor Based on High Q Fano Resonance of Nano-Patterned Electro-Optic Materials
Authors: Yin Xiaowei , Liu Fengli , Qiu Wentao , Liu Can , Guan Heyuan , Lu Huihui
Published: June 2022

This paper presents theoretical studies of Fano resonance based electric-field (E-field) sensors. E-field sensor based on two electro-optical (EO) materials i.e., barium titanate (BaTiO3, BTO) nanoparticles and relaxor ferroelectric material Pb(Mg1/3Nb2/3)O3-PbTiO3 (PMN-PT) combined with nanostructure are studied. As for the BTO based E-field sensor, a configuration of filling the BTO nanoparticles into a nano-patterned thin film silicon is proposed. The achieved resonance quality factor (Q) is 11,855 and a resonance induced electric field enhancement factor is of around 105. As for the design of PMN-PT based E-field sensor, a configuration by combining two square lattice air holes in PMN-PT thin film but with one offsetting hole left is chosen. The achieved resonance Q is of 9,273 and an electric field enhancement factor is of around 96. The resonance wavelength shift sensitivity of PMN-PT nanostructured can reach up to 4.768 pm/(V/m), while the BTO based nanostructure has a sensitivity of 0.1213 pm/(V/m). If a spectrum analyzer with 0.1 pm resolution is considered, then the minimum detection of the electric field Emin is 20 mV/m and 0.82 V/m for PMN-PT and BTO based nanostructures, respectively. The nano-patterned E-field sensor studied here are all dielectric, it has therefore the advantage of large measurement bandwidth, high measurement fidelity, high spatial resolution and high sensitivity.
Advanced Polymer Simulation and Processing
Authors: Fernandes Célio , Faroughi Salah A. , Ferrás Luís L. , Afonso Alexandre M.
Published: June 2022

Polymer processing techniques are of paramount importance in the manufacture of polymer parts [...]
Effect of Shear Angle and Printing Orientation on Shear Constitutive Response of Additively Manufactured Acrylonitrile Butadiene Styrene
Authors: Letizia Joshua , Chalivendra Vijaya , Li Dapeng
Published: June 2022

An experimental investigation was performed to understand the quasi-static shear response of additively manufactured (AM) acrylonitrile butadiene styrene (ABS) via fusion deposition modeling (FDM). A modified flat hat-shaped (FHS) specimen configuration was used for shear testing. The main aim of this study was to investigate the effect of four different shear angles (0°, 5.44°, 13.39°, and 20.83°) and three printing orientations (vertical build, 0°/90°, and 45°/−45°) on the shear constitutive response and shear performance of FDM-printed ABS. Scanning electron microscopy images of the failure surface were used to explain the shear response of the material. The flow shear stress of the shear stress-strain response for vertically printed specimens demonstrated a monotonic increase up to a peak shear stress and then decrease at the end of the shear zone, while for 0°/90° specimens, an increasing trend until the peak value at the end of the shear zone was observed. With increasing shear angles, all specimens printed with three printing orientations exhibited increasing shear zone size and shear strength, and the 0°/90° specimens exhibited the highest shear strength for all four shear angles. However, the specimens of the 45°/−45° orientation demonstrated the highest increase in shear strength by about 60% and in the shear strain at the end of shear zone by about 175% as the shear angle was increased from 0° to 20.83°.
Characterization, Antioxidant Activities, and Functional Properties of Mucilage Extracted from Corchorus olitorius L.
Authors: Oh Songmin , Kim Do-Yeong
Published: June 2022

This study extracted the mucilage from Corchorus olitorius L. to observe its chemical and functional properties and suggest its possible applications in various fields. Corchorus olitorius L. mucilage was isolated by hot water extraction. FT-IR and HPAEC-PAD were used to describe the chemical composition, and the functional properties and antioxidant activities of the mucilage were also examined. The mucilage was mainly composed of uronic acid (34.24%, w/w). The solubility was 79.48 ± 1.08% at 65 °C, the swelling index was 29.01 ± 2.54% at 25 °C, and the water-holding capacity and oil-binding capacity were 28.66 ± 1.48 and 8.423 ± 0.23 g/g, respectively. The mucilage viscosity increased from 4.38 to 154.97 cP in a concentration-dependent manner. Increasing the concentration decreased the emulsion activity and increased the emulsion stability, most likely because of the corresponding increase in surface tension and viscosity. Results from antioxidant assays confirmed that the in-vitro radical scavenging activity of the mucilage increased with concentration. This study shows that C. olitorius L. can be utilized as a new hydrocolloid source, with potential applications in fields ranging from foods to cosmetics and pharmaceuticals.