bluuebunny/crossref_metadata_2025 huggingface dataset:
Predicting Dyslexia and Reading Speed in Adolescents from Eye Movements in Reading and Non-Reading Tasks: A Machine Learning Approach
Authors: El Hmimdi Alae Eddine , Ward Lindsey M , Palpanas Themis , Kapoula Zoï
Published: October 2021

There is evidence that abnormalities in eye movements exist during reading in dyslexic individuals. A few recent studies applied Machine Learning (ML) classifiers to such eye movement data to predict dyslexia. A general problem with these studies is that eye movement data sets are limited to reading saccades and fixations that are confounded by reading difficulty, e.g., it is unclear whether abnormalities are the consequence or the cause of reading difficulty. Recently, Ward and Kapoula used LED targets (with the REMOBI & AIDEAL method) to demonstrate abnormalities of large saccades and vergence eye movements in depth demonstrating intrinsic eye movement problems independent from reading in dyslexia. In another study, binocular eye movements were studied while reading two texts: one using the “Alouette” text, which has no meaning and requires word decoding, the other using a meaningful text. It was found the Alouette text exacerbates eye movement abnormalities in dyslexics. In this paper, we more precisely quantify the quality of such eye movement descriptors for dyslexia detection. We use the descriptors produced in the four different setups as input to multiple classifiers and compare their generalization performances. Our results demonstrate that eye movement data from the Alouette test predicts dyslexia with an accuracy of 81.25%; similarly, we were able to predict dyslexia with an accuracy of 81.25% when using data from saccades to LED targets on the Remobi device and 77.3% when using vergence movements to LED targets. Noticeably, eye movement data from the meaningful text produced the lowest accuracy (70.2%). In a subsequent analysis, ML algorithms were applied to predict reading speed based on eye movement descriptors extracted from the meaningful reading, then from Remobi saccade and vergence tests. Remobi vergence eye movement descriptors can predict reading speed even better than eye movement descriptors from the meaningful reading test.
Safety and Efficacy of Eculizumab Therapy in Multiple Sclerosis: A Case Series
Authors: Allinovi Marco , Bellinvia Angelo , Pesce Francesco , Milan Manani Sabrina , Razzolini Lorenzo , Brezzi Brigida , Protopapa Paolo , Mantero Vittorio , Caroti Leonardo , Cirami Calogero Lino , Amato Maria Pia , Del Vecchio Lucia
Published: October 2021

(1) Background: Complement system activation has been proposed as one of the different factors that contribute to Multiple Sclerosis (MS) pathogenesis. In this study, we aimed to describe the potential effects of eculizumab, an anticomplement therapy, on MS disease activity in a cohort of relapsing–remitting (RR) MS patients who discontinued IFN-β therapy due to IFN-β-related thrombotic microangiopathy (TMA) onset. (2) Methods: In this retrospective observational multicentric study, we searched for all patients with MS treated by eculizumab with a survey of several nephrological and neurological centers (over 45 centers). (3) Results: Nine patients were included. The mean follow-up time under eculizumab was 3.72 ± 2.58 years. There were no significant differences in disease activity (EDSS, relapses, new T2, and/or Gd-enhancing lesions at MRI) considering the two years before and after eculizumab therapy. No adverse events potentially related to eculizumab therapy were reported during follow-up. (4) Conclusions: In this preliminary study, we described a good safety profile for eculizumab therapy in MS. However, the available data are not sufficient to make firm conclusions about the possible efficacy of eculizumab as a disease-modifying therapy for MS patients.
Psychiatric Disorders among the Military in West Africa: A Systematic Narrative Review
Authors: Asare-Doku Winifred , Donnir Gordon Maanianu , Ayuurebobi Ae-Ngibise Kenneth , Peprah Jennifer , Awuviry-Newton Kofi , Acquah Francis
Published: October 2021

(1) Background: Military combat impacts the mental health of veterans and active military personnel. Although various studies, the majority of which are from Westernized countries, have provided insight into how combat causes psychiatric disorders, such as post-traumatic stress disorder (PTSD), depression, and anxiety among veterans, there appears to be scant published literature on psychiatric disorders among military personnel in the West African region. It is important to contextually understand the psychiatric disorders among military personnel in this region who may be faced with similar vulnerabilities to their counterparts from Western cultures. (2) Methods: This study follows the Preferred Reporting Items for Systematic reviews and Meta-Analyses (PRISMA) guidelines. Studies were included if they were published in English between January 2010 and January 2021 and assessed mental health problems or psychiatric disorders among soldiers in West Africa. (3) Results: All three included studies were from Nigeria. High prevalence rates of substance and alcohol use were reported. (4) Conclusions: This review highlights the need for more research in this population as well as identifying the intervention needs of the soldiers and their implications.
Sustainable Urban Agriculture as Functional Hybrid Unit—Issues of Urban Resilience
Authors: Grochulska-Salak Magdalena , Nowysz Aleksandra , Tofiluk Anna
Published: October 2021

The aim of the research is to present a review of urban agriculture as synergic green and blue infrastructure solutions and to evaluate modern hybrid units with biomass and food production, and water retention in urbanized areas. The synergy between technologies of biomass production and water reuse provides the basis for the idea of self-sufficient urban units and sustainable agriculture. The research work defines the criteria and typology for urban resilience solutions. The analyses concern the correlation between production, management, retention, and reuse of water as a part of solutions for the model of a sustainable urban agriculture system in a compact city. The obtained results describe typology for cultivation and production in the modern city. Creating a resilient city connected with requirements posed by civilization concern changes in functional and spatial structure of the compact city. The discussion is supplemented with conclusions to the issue of synergy in urban planning, architecture, and engineering solutions. The article describes implementation technologies for city resilience in the context of agricultural production, energy and water management for the local community, and the ecosystem services in the city.
Optimization of SPIO Injection for Sentinel Lymph Node Dissection in a Rat Model
Authors: Peek Mirjam C. L. , Saeki Kohei , Ohashi Kaichi , Chikaki Shinichi , Baker Rose , Nakagawa Takayuki , Kusakabe Moriaki , Douek Michael , Sekino Masaki
Published: October 2021

The magnetic technique, consisting of a magnetic tracer and a handheld magnetometer, is a promising alternative technique for sentinel lymph node dissection (SLND) and was shown to be non-inferior to the standard technique in terms of identification rates. In this study, injection characteristics (iron dose, dilution, time course and massaging) were evaluated to optimize magnetic tracer uptake in the sentinel lymph nodes (SLN) in a rat hindleg model. 202 successful SLNDs were performed. Iron uptake in the SLN is proportional (10% utilization rate) to the injection dose between 20 and 200 μg, showing a plateau uptake of 80 μg in the SLN around 1000 μg injection. Linear regression showed that time had a higher impact than dilution, on the SLN iron uptake. Massaging showed no significant change in iron uptake. The amount of residual iron at the injection site was also proportional to the injection dose without any plateau. Time was a significant factor for wash-out of residual iron. From these results, preoperative injection may be advantageous for SLN detection as well as reduction in residual iron at the injection site by potential decrease in required injection dose.
High-Fat Diet-Induced Obese Effects of Adipocyte-Specific CXCR2 Conditional Knockout in the Peritoneal Tumor Microenvironment of Ovarian Cancer
Authors: Choe Deokyeong , Lee Eun-Sook , Beeghly-Fadiel Alicia , Wilson Andrew J. , Whalen Margaret M. , Adunyah Samuel E. , Son Deok-Soo
Published: October 2021

Obesity contributes to ovarian cancer (OC) progression via tumorigenic chemokines. Adipocytes and OC cells highly express CXCR2, and its ligands CXCL1/8, respectively, indicating that the CXCL1/8-CXCR2 axis is a molecular link between obesity and OC. Here, we investigated how the adipocyte-specific CXCR2 conditional knockout (cKO) affected the peritoneal tumor microenvironment of OC in a high-fat diet (HFD)-induced obese mouse model. We first generated adipocyte-specific CXCR2 cKO in mice: adipose tissues were not different in crown-like structures and adipocyte size between the wild-type (WT) and cKO mice but expressed lower levels of CCL2/6 compared to the obese WT mice. HFD-induced obese mice had a shorter survival time than lean mice. Particularly, obese WT and cKO mice developed higher tumors and ascites burdens, respectively. The ascites from the obese cKO mice showed increased vacuole clumps but decreased the floating tumor burden, tumor-attached macrophages, triglyceride, free fatty acid, CCL2, and TNF levels compared to obese WT mice. A tumor analysis revealed that obese cKO mice attenuated inflammatory areas, PCNA, and F4/80 compared to obese WT mice, indicating a reduced tumor burden, and there were positive relationships between the ascites and tumor parameters. Taken together, the adipocyte-specific CXCR2 cKO was associated with obesity-induced ascites despite a reduced tumor burden, likely altering the peritoneal tumor microenvironment of OC.
In silico Approach for Validating and Unveiling New Applications for Prognostic Biomarkers of Endometrial Cancer
Authors: Coll-de la Rubia Eva , Martinez-Garcia Elena , Dittmar Gunnar , Nazarov Petr V. , Bebia Vicente , Cabrera Silvia , Gil-Moreno Antonio , Colás Eva
Published: October 2021

Endometrial cancer (EC) mortality is directly associated with the presence of prognostic factors. Current stratification systems are not accurate enough to predict the outcome of patients. Therefore, identifying more accurate prognostic EC biomarkers is crucial. We aimed to validate 255 prognostic biomarkers identified in multiple studies and explore their prognostic application by analyzing them in TCGA and CPTAC datasets. We analyzed the mRNA and proteomic expression data to assess the statistical prognostic performance of the 255 proteins. Significant biomarkers related to overall survival (OS) and recurrence-free survival (RFS) were combined and signatures generated. A total of 30 biomarkers were associated either to one or more of the following prognostic factors: histological type (n = 15), histological grade (n = 6), FIGO stage (n = 1), molecular classification (n = 16), or they were associated to OS (n = 11), and RFS (n = 5). A prognostic signature composed of 11 proteins increased the accuracy to predict OS (AUC = 0.827). The study validates and identifies new potential applications of 30 proteins as prognostic biomarkers and suggests to further study under-studied biomarkers such as TPX2, and confirms already used biomarkers such as MSH6, MSH2, or L1CAM. These results are expected to advance the quest for biomarkers to accurately assess the risk of EC patients.
Body Fat Distribution and Risk of Breast, Endometrial, and Ovarian Cancer: A Two-Sample Mendelian Randomization Study
Authors: Freuer Dennis , Linseisen Jakob , O’Mara Tracy A. , Leitzmann Michael , Baurecht Hansjörg , Baumeister Sebastian-Edgar , Meisinger Christa
Published: October 2021

Background: Mounting evidence shows that adiposity increases female-specific cancer risk, but the role of body fat distribution is less clear. We used a two-sample Mendelian randomization (MR) approach to elucidate causal relations of body fat distribution to the risks of breast, endometrial and ovarian cancers and their subtypes. Methods: Body composition was assessed using segmental bioelectrical impedance analysis, yielding trunk, arm, and leg fat ratios (TFR, AFR, LFR) and BMI including 195,043 and 434,794 European women, respectively. The sample sizes for the outcomes ranged between 58,396 and 228,951. Causal effects were estimated per one standard deviation increment in the respective exposure within the radial regression framework. Robust sensitivity analyses were performed to verify MR assumptions. In a multivariable MR setting, the proportion of risk attributable to overall and abdominal fat content was assessed. Results: TFR, which represents abdominal fat content, was associated with ovarian cancer and its clear cell and endometrioid histotypes independent of overall fat content. BMI was inversely associated with breast cancer and its ER− and ER+ subtypes, but positively with endometrial cancer and ovarian cancer, including its endometrioid histotype. These estimates were confirmed using AFR as proxy for overall body fat. Conclusions: Visceral adiposity seems to be a driver of elevated ovarian cancer risk, particularly of the endometrioid and clear cell ovarian cancer histotypes. General adiposity decreases the risk of breast cancer but increases the risk of endometrial and ovarian cancer.
E-Cadherin Regulates Mitochondrial Membrane Potential in Cancer Cells
Authors: Begum Hydari Masuma , Mariano Chelsea , Zhou Hao , Shen Keyue
Published: October 2021

Epithelial cancer cells often have unusually higher mitochondrial membrane potential (ΔΨm) than their normal counterparts, which has been associated with increased invasiveness in vitro and higher metastatic potential in vivo. However, the mechanisms by which ΔΨm in cancer cells is regulated in tumor microenvironment (TME) remain unclear. In this study, we used an in vitro micropatterning platform to recapitulate biophysical confinement cues in the TME and investigated the mechanisms by which these regulate cancer cell ΔΨm. We found that micropatterning resulted in a spatial distribution of ΔΨm, which correlated with the level of E-cadherin mediated intercellular adhesion. There was a stark contrast in the spatial distribution of ΔΨm in the micropattern of E-cadherin-negative breast cancer cells (MDA-MB-231) compared to that of the high E-cadherin expressing (MCF-7) cancer cells. Disruption and knockout of E-cadherin adhesions rescued the low ΔΨm found at the center of MCF-7 micropatterns with high E-cadherin expression, while E-cadherin overexpression in MDA-MB-231 and MCF-7 cells lowered their ΔΨm at the micropattern center. These results show that E-cadherin plays an important role in regulating the ΔΨm of cancer cells in the context of biophysical cues in TME.
Periostin Exon-21 Antibody Neutralization of Triple-Negative Breast Cancer Cell-Derived Periostin Regulates Tumor-Associated Macrophage Polarization and Angiogenesis
Authors: Fujikawa Tatsuya , Sanada Fumihiro , Taniyama Yoshiaki , Shibata Kana , Katsuragi Naruto , Koibuchi Nobutaka , Akazawa Kaori , Kanemoto Yuko , Kuroyanagi Hidehito , Shimazu Kenzo , Rakugi Hiromi , Morishita Ryuichi
Published: October 2021

Periostin (Pn) is involved in multiple processes of cancer progression. Previously, we reported that Pn expression is correlated with mesenchymal tumor markers and poor prognosis in triple-negative breast cancer (TNBC). In the TNBC xenograft model, chemotherapy increased expression of a Pn alternative splicing variant (ASV) with exon 21, and administration of the neutralizing antibody against Pn with exon 21 (Pn-21 Ab) overcame chemoresistance with a reduction in the mesenchymal cancer cell fraction. In the present study, the role of Pn ASV with exon 21 in TNBC progression has been addressed. We first established a stable cell line carrying a fluorescence-based splicing reporter. Pn-positive TNBC has higher expression of genes related to tumor-associated macrophage (TAM) recruitment and ECM-receptor interaction than Pn-negative cells. In a xenograft model, only Pn-positive cells initiated tumor formation, and the Pn-21 Ab suppressed tumor cell growth, accompanied by decreased M2 TAM polarization and the number of tumor vessels. These data suggest that cancer cell-derived Pn ASV educates TAMs and regulates angiogenesis, which in turn establishes a microenvironmental niche that is supportive of TNBC.
BRAFV600E Mutant Allele Frequency (MAF) Influences Melanoma Clinicopathologic Characteristics
Authors: Soria Xavier , Vilardell Felip , Maiques Óscar , Barceló Carla , Sisó Pol , de la Rosa Inés , Velasco Ana , Cuevas Dolors , Santacana Maria , Gatius Sònia , Matías-Guiu Xavier , Rodrigo Alberto , Macià Anna , Martí Rosa M.
Published: October 2021

Background: Cutaneous melanoma shows high variability regarding clinicopathological presentation, evolution and prognosis. Methods: Next generation sequencing was performed to analyze hotspot mutations in different areas of primary melanomas (MMp) and their paired metastases. Clinicopathological features were evaluated depending on the degree of variation of the BRAFV600E mutant allele frequency (MAF) in MMp. Results: In our cohort of 14 superficial spreading, 10 nodular melanomas and 52 metastases, 17/24 (71%) melanomas had a BRAFV600E mutation and 5/24 (21%) had a NRASQ61 mutation. We observed a high variation of BRAFV600E MAF (H-BRAFV600E) in 7/17 (41%) MMp. The H-BRAFV600E MMp were all located on the trunk, had lower Breslow and mitotic indexes and predominantly, a first nodal metastasis. Regions with spindled tumor cells (Spin) and high lymphocytic infiltrate (HInf) were more frequent in the H-BRAFV600E patients (4/7; 57%), whereas regions with epithelial tumor cells (Epit) and low lymphocytic infiltrate (LInf) were predominant (6/10; 60%) and exclusive in the low BRAFV600E MAF variation tumors (L-BRAFV600E). The H-BRAFV600E/Spin/HInf MMp patients had better prognostic features and nodal first metastasis. Conclusions: The H-BRAFV600E MMp were located on the trunk, had better prognostic characteristics, such as lower Breslow and mitotic indexes as well as high lymphocytic infiltrate.
Quality of Life and Symptom Management in Advanced Biliary Tract Cancers
Authors: Hunter Lindsay A. , Soares Heloisa P.
Published: October 2021

Biliary tract carcinomas (BTCs) account for less than 1% of all cancers but are increasing in incidence. Prognosis is poor for BTC patients, with 5-year survival rates of less than 10%. While chemotherapy has been the mainstay treatment for patients with advanced BTC, immunotherapy and targeted therapies are being evaluated in numerous clinical trials and rapidly incorporated into clinical practice. As patients with BTC have reduced health-related quality of life (HRQoL) due to both tumor- and treatment-related symptoms, it is important for clinicians to recognize and manage these symptoms early. This review will highlight the anticipated complications from BTC and its systemic treatment, as well as their effects on HRQoL.
Evolving Therapeutic Approaches for Older Patients with Acute Myeloid Leukemia in 2021
Authors: Urbino Irene , Secreto Carolina , Olivi Matteo , Apolito Vincenzo , D’Ardia Stefano , Frairia Chiara , Giai Valentina , Aydin Semra , Freilone Roberto , Dellacasa Chiara , Giaccone Luisa , Ferrero Dario , Audisio Ernesta , Busca Alessandro , Cerrano Marco
Published: October 2021

Acute myeloid leukemia (AML) in older patients is characterized by unfavorable prognosis due to adverse disease features and a high rate of treatment-related complications. Classical therapeutic options range from intensive chemotherapy in fit patients, potentially followed by allogeneic hematopoietic cell transplantation (allo-HCT), to hypomethylating agents or palliative care alone for unfit/frail ones. In the era of precision medicine, the treatment paradigm of AML is rapidly changing. On the one hand, a plethora of new targeted drugs with good tolerability profiles are becoming available, offering the possibility to achieve a prolonged remission to many patients not otherwise eligible for more intensive therapies. On the other hand, better tools to assess patients’ fitness and improvements in the selection and management of those undergoing allo-HCT will hopefully reduce treatment-related mortality and complications. Importantly, a detailed genetic characterization of AML has become of paramount importance to choose the best therapeutic option in both intensively treated and unfit patients. Finally, improving supportive care and quality of life is of major importance in this age group, especially for the minority of patients that are still candidates for palliative care because of very poor clinical conditions or unwillingness to receive active treatments. In the present review, we discuss the evolving approaches in the treatment of older AML patients, which is becoming increasingly challenging following the advent of new effective drugs for a very heterogeneous and complex population.
Metal Nanoparticle Catalysis
Authors: Lara Patricia , Martínez-Prieto Luis M.
Published: October 2021

In recent years, the catalytic use of metal nanoparticles (MNPs) has experienced a growing interest [...]
Cu/ZSM5-Geopolymer 3D-Printed Monoliths for the NH3-SCR of NOx
Authors: Cepollaro Elisabetta Maria , Botti Renata , Franchin Giorgia , Lisi Luciana , Colombo Paolo , Cimino Stefano
Published: October 2021

Geopolymer-based monoliths manufactured by direct ink writing, containing up to 60% by weight of presynthesized ZSM5 with low Si/Al ratio, were investigated as structured catalysts for the NH3-SCR of NOx. Copper was introduced as the active metal by ion exchange after a preliminary acid treatment of the monoliths. Monolithic catalysts were characterized by morphological (XRD and SEM), textural (BET and pore size distribution), mechanical (compressive strength), chemical (ICP–MS), redox (H2-TPR) and surface (NH3-TPD) analyses, showing the preservation of Cu-exchanged zeolite features in the composite monoliths. NH3-SCR tests, carried out on both monolithic and powdered samples in the temperature range 70–550 °C, confirmed that composite monoliths provide a very good activity and a high selectivity to N2 over the whole range of temperatures explored due to the hierarchical structure of the materials, in addition to a good mechanical resistance—mostly related to the geopolymer matrix.
Novel Recyclable Pd/H-MOR Catalyst for Suzuki-Miyaura Coupling and Application in the Synthesis of Crizotinib
Authors: Zhou Enmu , Jin Jianzhong , Zheng Kai , Zhang Letian , Xu Hao , Shen Chao
Published: October 2021

In this paper, we report an effective ultrasound method for the synthesis of Pd/H-MOR, which was used as a catalyst in the Suzuki-Miyaura coupling of aryl halides with phenylboronic acid. The structure and morphology of the as-prepared catalysts were fully characterized by X-ray diffraction (XRD), N2 sorption isotherms, scanning electron microscopy (SEM), and an inductively coupled plasma-atomic emission spectrometer (ICP-AES). The advantages of Pd/H-MOR in the coupling reaction are green solvents, high yields, absence of ligands, and recyclability. The catalysts were easily reused at least ten times without significant deterioration in catalytic activity. In addition, this protocol was used in the marketed anti-tumor drug crizotinib synthesis.
Isolation and Identification of an Efficient Aerobic Denitrifying Pseudomonas stutzeri Strain and Characterization of Its Nitrite Degradation
Authors: Fu Weilai , Wang Qiang , Chen Shuhui , Wang Yunshuang , Wang Yaru , Duan Peifeng , Yi Ganfeng , Liu Chao , Zhang Xian , Rao Zhiming
Published: October 2021

Nitrogen pollution in water bodies is becoming increasingly serious, and how to remove nitrogen from water bodies economically and effectively has become a research hotspot. Especially in recent years, with the gradual expansion of aquaculture in China, the content of nitrite and other nitrogen-containing substances in water bodies has been increasing, which inhibits the growth of farm animals and is one of the causes of eutrophication in water bodies. In this study, a strain of bacteria was isolated from the sludge of an aquaculture fishpond and identified as Pseudomonas stutzeri, which can efficiently degrade nitrite. After continuous domestication in nitrite mixed solution, the nitrite nitrogen reduction capacity of P. stutzeri was significantly improved. Univariate experiments aiming to optimize the degradation conditions indicate that the optimal culture conditions for strain F2 are: medium with a carbon source of sodium succinate; C/N of 18; pH of 8; culture temperature of 28 °C; and shaking speed of 210 rpm in the shaker. Under the optimal culture conditions, the NO2−-N concentration of the culture solution was 300 mg/L, and the nitrite removal rate reached 98.67%. Meanwhile, the results of the nitrogen balance test showed that the strain converted 6.1% of the initial nitrogen into cellular organic nitrogen and 62.3% into gaseous nitrogen.
Promoting Role of Amorphous Carbon and Carbon Nanotubes Growth Modes of Methane Decomposition in One-Pot Catalytic Approach
Authors: Chen Lifang , Noreña Luis Enrique , Wang Jin An , Limas Roberto , Arellano Ulises , González Vargas Oscar Arturo
Published: October 2021

We report the simultaneous production of hydrogen fuel and carbon nanotubes (CNTs) via methane dehydrogenation catalyzed with Ni/SBA-15. Most Ni nanoparticles (NPs) with size between 10 and 30 nm were highly dispersed on SBA-15 and most of them had a strong interaction with the support. At temperatures ranging from 500 to 800 °C, methane could be decomposed to release hydrogen with 100% selectivity at conversion between 51 and 65%. There was no CO or CO2 detectable in the reaction fluent. In the initial stage of the reaction, amorphous carbon and dehydrogenated methane species adsorbed on the Ni NPs promoted the CH4 decomposition. The amorphous carbon atoms were then transformed into carbon nanotubes which chiefly consisted of a multiwall structure and grew towards different orientations via a tip-growth or a base-growth modes, controlled by the interaction strength between the Ni NPs and the SBA-15 support. Reaction temperature affected not only methane conversion, but also the diffusion of carbon atoms on/in the Ni NPs and their precipitation at the interfaces. At higher temperature, bamboo-like CNTs or onion-like metal-encapsulated carbons were formed, mainly due to the rate of carbon atom formation greater than that of carbon precipitation for CNTs construction. The CNTs formation mechanisms are discussed and their growth modes under different conditions are proposed.
Recent Advances in the Catalytic Treatment of Volatile Organic Compounds: A Review Based on the Mixture Effect
Authors: Rochard Guillaume , Olivet Lilian , Tannous Mariebelle , Poupin Christophe , Siffert Stéphane , Cousin Renaud
Published: October 2021

Catalytic total oxidation is an efficient technique for treating VOCs, which are mainly emitted by solvent-based industrial processes. However, studies of the catalytic oxidation of VOCs in combination with other pollutants are very limited, despite the fact that this is a key step of knowledge before industrial application. During the oxidation reaction, the behavior of a molecule may change depending on the reaction mixture. For the treatment of an effluent loaded with VOCs, it is necessary to carefully select not only the catalytic material to be used but also the reaction conditions. Indeed, the catalytic oxidation of a component in a VOCs mixture is not predicted solely from the behavior of individual component. Thus, the objective of this small review is to carry out a study on the effect observed in the case of the oxidation of a VOCs mixture or in the presence of water, NOX or sulfur compounds.
Angiotensin II-Induced Long Non-Coding RNA Alivec Regulates Chondrogenesis in Vascular Smooth Muscle Cells
Authors: Samara Vishnu Amaram , Das Sadhan , Reddy Marpadga A. , Tanwar Vinay Singh , Stapleton Kenneth , Leung Amy , Abdollahi Maryam , Ganguly Rituparna , Lanting Linda , Natarajan Rama
Published: October 2021

Long non-coding RNAs (lncRNAs) play key roles in Angiotensin II (AngII) signaling but their role in chondrogenic transformation of vascular smooth muscle cells (VSMCs) is unknown. We describe a novel AngII-induced lncRNA Alivec (Angiotensin II-induced lncRNA in VSMCs eliciting chondrogenic phenotype) implicated in VSMC chondrogenesis. In rat VSMCs, Alivec and the nearby gene Acan, a chondrogenic marker, were induced by growth factors AngII and PDGF and the inflammatory cytokine TNF-α. AngII co-regulated Alivec and Acan through the activation of AngII type1 receptor signaling and Sox9, a master transcriptional regulator of chondrogenesis. Alivec knockdown with GapmeR antisense-oligonucleotides attenuated the expression of AngII-induced chondrogenic marker genes, including Acan, and inhibited the chondrogenic phenotype of VSMCs. Conversely, Alivec overexpression upregulated these genes and promoted chondrogenic transformation. RNA-pulldown coupled to mass-spectrometry identified Tropomyosin-3-alpha and hnRNPA2B1 proteins as Alivec-binding proteins in VSMCs. Furthermore, male rats with AngII-driven hypertension showed increased aortic expression of Alivec and Acan. A putative human ortholog ALIVEC, was induced by AngII in human VSMCs, and this locus was found to harbor the quantitative trait loci affecting blood pressure. Together, these findings suggest that AngII-regulated lncRNA Alivec functions, at least in part, to mediate the AngII-induced chondrogenic transformation of VSMCs implicated in vascular dysfunction and hypertension.
Apoptotic Cells Trigger Calcium Entry in Phagocytes by Inducing the Orai1-STIM1 Association
Authors: Kim Deokhwan , Moon Hyunji , Cho Hyeokjin , Min Chanhyuk , Moon Byeongjin , Yang Susumin , Lee Juyeon , Lee Sang-Ah , Park Hyunjin , Lee Dae-Hee , Jeong Dongtak , Lee Gwangrog , Park Daeho
Published: October 2021

Swift and continuous phagocytosis of apoptotic cells can be achieved by modulation of calcium flux in phagocytes. However, the molecular mechanism by which apoptotic cells modulate calcium flux in phagocytes is incompletely understood. Here, using biophysical, biochemical, pharmaceutical, and genetic approaches, we show that apoptotic cells induced the Orai1-STIM1 interaction, leading to store-operated calcium entry (SOCE) in phagocytes through the Mertk-phospholipase C (PLC) γ1-inositol 1,4,5-triphosphate receptor (IP3R) axis. Apoptotic cells induced calcium release from the endoplasmic reticulum, which led to the Orai1-STIM1 association and, consequently, SOCE in phagocytes. This association was attenuated by masking phosphatidylserine. In addition, the depletion of Mertk, which indirectly senses phosphatidylserine on apoptotic cells, reduced the phosphorylation levels of PLCγ1 and IP3R, resulting in attenuation of the Orai1-STIM1 interaction and inefficient SOCE upon apoptotic cell stimulation. Taken together, our observations uncover the mechanism of how phagocytes engulfing apoptotic cells elevate the calcium level.
Transcriptomic Biomarkers for Tuberculosis: Validation of NPC2 as a Single mRNA Biomarker to Diagnose TB, Predict Disease Progression, and Monitor Treatment Response
Authors: de Araujo Leonardo S. , Ribeiro-Alves Marcelo , Wipperman Matthew F. , Vorkas Charles Kyriakos , Pessler Frank , Saad Maria Helena Féres
Published: October 2021

External validation in different cohorts is a key step in the translational development of new biomarkers. We previously described three host mRNA whose expression in peripheral blood is significantly higher (NPC2) or lower (DOCK9 and EPHA4) in individuals with TB compared to latent TB infection (LTBI) and controls. We have now conducted an independent validation of these genes by re-analyzing publicly available transcriptomic datasets from Brazil, China, Haiti, India, South Africa, and the United Kingdom. Comparisons between TB and control/LTBI showed significant differential expression of all three genes (NPC2high p < 0.01, DOCK9low p < 0.01, and EPHA4low p < 0.05). NPC2high had the highest mean area under the ROC curve (AUROC) for the differentiation of TB vs. controls (0.95) and LTBI (0.94). In addition, NPC2 accurately distinguished TB from the clinically similar conditions pneumonia (AUROC, 0.88), non-active sarcoidosis (0.87), and lung cancer (0.86), but not from active sarcoidosis (0.66). Interestingly, individuals progressing from LTBI to TB showed a constant increase in NPC2 expression with time when compared to non-progressors (p < 0.05), with a significant change closer to manifestation of active disease (≤3 months, p = 0.003). Moreover, NPC2 expression normalized with completion of anti-TB treatment. Taken together, these results validate NPC2 mRNA as a diagnostic host biomarker for active TB independent of host genetic background. Moreover, they reveal its potential to predict progression from latent to active infection and to indicate a response to anti-TB treatment.
Redox Control of the Dormant Cancer Cell Life Cycle
Authors: Li Bowen , Huang Yichun , Ming Hui , Nice Edouard C. , Xuan Rongrong , Huang Canhua
Published: October 2021

Following efficient tumor therapy, some cancer cells may survive through a dormancy process, contributing to tumor recurrence and worse outcomes. Dormancy is considered a process where most cancer cells in a tumor cell population are quiescent with no, or only slow, proliferation. Recent advances indicate that redox mechanisms control the dormant cancer cell life cycle, including dormancy entrance, long-term dormancy, and metastatic relapse. This regulatory network is orchestrated mainly through redox modification on key regulators or global change of reactive oxygen species (ROS) levels in dormant cancer cells. Encouragingly, several strategies targeting redox signaling, including sleeping, awaking, or killing dormant cancer cells are currently under early clinical evaluation. However, the molecular mechanisms underlying redox control of the dormant cancer cell cycle are poorly understood and need further exploration. In this review, we discuss the underlying molecular basis of redox signaling in the cell life cycle of dormant cancer and the potential redox-based targeting strategies for eliminating dormant cancer cells.
The SZT2 Interactome Unravels New Functions of the KICSTOR Complex
Authors: Cattelani Cecilia , Lesiak Dominik , Liebscher Gudrun , Singer Isabel I. , Stasyk Taras , Wallnöfer Moritz H. , Heberle Alexander M. , Corti Corrado , Hess Michael W. , Pfaller Kristian , Kwiatkowski Marcel , Pramstaller Peter P. , Hicks Andrew A. , Thedieck Kathrin , Müller Thomas , Huber Lukas A. , Eca Guimaraes de Araujo Mariana
Published: October 2021

Seizure threshold 2 (SZT2) is a component of the KICSTOR complex which, under catabolic conditions, functions as a negative regulator in the amino acid-sensing branch of mTORC1. Mutations in this gene cause a severe neurodevelopmental and epileptic encephalopathy whose main symptoms include epilepsy, intellectual disability, and macrocephaly. As SZT2 remains one of the least characterized regulators of mTORC1, in this work we performed a systematic interactome analysis under catabolic and anabolic conditions. Besides numerous mTORC1 and AMPK signaling components, we identified clusters of proteins related to autophagy, ciliogenesis regulation, neurogenesis, and neurodegenerative processes. Moreover, analysis of SZT2 ablated cells revealed increased mTORC1 signaling activation that could be reversed by Rapamycin or Torin treatments. Strikingly, SZT2 KO cells also exhibited higher levels of autophagic components, independent of the physiological conditions tested. These results are consistent with our interactome data, in which we detected an enriched pool of selective autophagy receptors/regulators. Moreover, preliminary analyses indicated that SZT2 alters ciliogenesis. Overall, the data presented form the basis to comprehensively investigate the physiological functions of SZT2 that could explain major molecular events in the pathophysiology of developmental and epileptic encephalopathy in patients with SZT2 mutations.
DLC-Based Coatings Obtained by Low-Frequency Plasma-Enhanced Chemical Vapor Deposition (LFPECVD) in Cyclohexane, Principle and Examples
Authors: Sanchette Frederic , El Garah Mohamed , Achache Sofiane , Schuster Frederic , Chouquet Caroline , Ducros Cédric , Billard Alain
Published: October 2021

The LFPECVD (Low-Frequency Plasma-Enhanced Chemical Vapor Deposition) technique is now used on an industrial scale for the deposition of carbon-based coatings for several applications. This short review recalled the main principles of LFPECVD and provided examples of DLC-based films. The main differences between low-frequency (LF) and radio-frequency (RF) discharges were also recalled here and examples of deposition and characterization of carbon-based films were proposed. The influence of the bias voltage or the temperature of the active electrode on the deposition rate and the structure of a-C: H films obtained in cyclohexane/hydrogen mixtures was first discussed. Next, the properties of carbon-based films doped with silicon were described and, finally, it was shown that multilayer architectures make it possible to reduce the stresses without altering their tribological properties.
Framework for Mitigation of Welding Induced Distortion through Response Surface Method and Reinforcement Learning
Authors: Waheed Rehan , Saeed Hasan Aftab , Butt Sajid Ullah , Anjum Bilal
Published: October 2021

Welding induced distortion causes dimensional inaccuracies in parts being produced and assembly fit-up problems during manufacturing. In this study, a framework is proposed to mitigate weld distortion at the design stage. A sequential approach is adopted to optimize the welding process. In the first phase, welding process parameters are optimized through the response surface method. The effect of these parameters on the overall distortion of the welded part is observed by a simulation of the welding process. In the second phase, the weld sequence is optimized using the optimum weld parameters. A reinforcement learning-based Q-learning technique is used to select the optimum welding path by sequential observation of weld distortion at each segment being welded. The optimum process parameters and weld path sequence have been selected for 3 mm steel plates having a lap joint configuration and a 2 mm vent panel with a butt joint configuration. It is concluded that the combination of the optimum welding parameters and welding sequence yields minimum distortion. By applying this framework, a reduction of 19% is observed in overall welding induced distortion.
Study on the Structural Characteristics of Bird Necks and Their Static Motion Features in the Sagittal Plane
Authors: Wang Jiajia , Jia Wenfeng , Zhang Fu , Ma Xiqiang , Qiu Zhaomei , Qian Zhihui , Ren Luquan , Guo Zhijun , Zhang Yakun
Published: October 2021

The necks of birds that possess complex structures, graceful curves, and flexible movements are perfect natural motion actuators. Studying their structural features, mechanic characteristics, and motion rules can provide valuable references for imitating such actuators and motion functions artificially. Previous studies have analyzed the influence of two-dimensional motion geometric features and anatomical structure of the neck on motion efficiency and motion stability. However, the mechanism of motion flexibility from the perspective of neck structure has not been investigated. This study investigates the general law of the relationship between the structural parameters and motion characteristics of birds’ necks using tomography technology and 3D reconstruction technology. The results show that the structural characteristics of geese and ducks are similar, and there are significant differences in joint motion characteristics. Geese obtains complex neck postures through active intervertebral joints and highly flexible facet joints and possesses higher neck flexibility than ducks. This study provides a generic measuring method for obtaining birds’ cervical spinal vertebral structural dimensional parameters and offers a new theoretical concept for bionic robotic structural design and manufacture.
Influence of Voltage Sags on the Continuity of the Operation and Lifespan of Single-Phase Industrial Robots
Authors: Putynkowski Grzegorz , Woźny Krzysztof , Szychta Elzbieta , Szychta Leszek
Published: October 2021

Due to the increase in the number of automated processes that employ industrial robots (especially in industrial and laboratory environments, including vacuum systems), and the resulting increase in the number of unresolved service requests, the purpose of the authors’ research was to confirm the occurrence of disturbances in the form of voltage sags that are not recorded by automation systems and which lead to the destruction of robots or their equipment in areas defined by the characteristics of ITIC/SEMI F47 and CBEMA as being free from such disturbances. The article also describes the environmental classification of robots by their process functionalities/features, and recommends equipment that is able to compensate for these disturbances. Such a classification approach can be an excellent tool for building an exploitation culture and assist the conscious selection of electrical equipment in robotised systems susceptible to disturbances (e.g., robots in load-lock in vacuum environment).
Effect of Modulation Periods on the Mechanical and Tribological Performance of MoS2–TiL/MoS2–TiH Multilayer Coatings
Authors: Zhang Ping , Ying Puyou , Lin Changhong , Yang Tao , Wu Jianbo , Huang Min , Wang Tianle , Fang Yihang , Levchenko Vladimir
Published: October 2021

MoS2–Ti coating is a widely used solid lubricant owing to its low friction coefficient. The mechanical and tribological performance of the coating can be further improved via introducing a multilayer structure, which is closely related to the modulation period and significantly affects the properties of the coating. Herein, the effect of two different modulation periods on the mechanical and tribological performance of the MoS2–TiL/MoS2–TiH multilayer coatings (where L and H represent low and high-powered sputtering of the titanium target) was studied. The performance of the coatings was found to depend on modulation periods of single layer thickness and thickness ratio, respectively. When the thickness ratio of MoS2–TiL layer to MoS2–TiH layer was fixed with different number of layers, the adverse effects of the interface outweighed the beneficial effect; thus, the mechanical and tribological performance of the multilayer coatings were improved with an increase in the single layer thickness. When the effect of the multilayer interfaces on the studied coatings was similar with the same number of layers, the MoS2–TiH layer had more impact on the hardness of the MoS2–TiL/MoS2–TiH multilayer coatings, whereas the MoS2–TiL layer substantially affected the adhesion properties, friction behavior and wear resistance. This study can provide a way to regulate coatings with different performance requirements via building different multilayer microstructures.
Spectroscopic Identification of Amber Imitations: Different Pressure and Temperature Treatments of Copal Resins
Authors: Zheng Ting , Li Haibo , Lu Taijin , Chen Xiaoming , Li Bowen , Liu Yingying
Published: October 2021

Copal resins can be treated with heat and/or pressure to imitate ambers in the gem market. To explore the effects of different modification conditions on post-treatment spectral changes, five experimental methods with different temperature–pressure parameters were designed to modify two types of copal resins. The treated copal resins were examined by infrared, Raman and nuclear magnetic resonance spectroscopy. Results indicate that all the treatment methods simulate the maturation process, with spectral characteristics becoming more similar to those of ambers. Multi-stage heat–pressure treatment has the most significant effect on Colombia and Madagascar copal resins, with their spectra being similar to those of Dominican and Mexican ambers. Rapid high-temperature treatment at 180 °C modified the Borneo copal resin, with its infrared spectrum developing a “Baltic shoulder” resembling that of heat-treated Baltic amber. Even though there are many similarities between treated copal resins and natural ambers, they can still be distinguished by spectroscopic methods.
Examining the Pathoplastic Moderating Role of Education on the Association between Depressive Mood and Self-Rated Health among Cancer Survivors: A Population-Based Study
Authors: Zhang Anao , Wang Kaipeng , DuVall Adam S.
Published: October 2021

Objective: Self-rated health (SRH) is a salient patient outcome for cancer survivors, and depressive mood and education are known determinants of cancer survivors’ SRH. Moving beyond the well-established direct association between depressive mood, education, and SRH among cancer survivors, this epidemiological study investigated the pathoplastic role of education on depressive mood in relation to SRH among a nationally representative sample of cancer survivors in the United States. Methods: The 2019 National Health Interview Survey was analyzed using data from adult participants (≥18 years old) who self-reported as cancer survivors (n = 3844). Ordered logistic regression was used to evaluate the direct impact of depressive mood and education in relation to SRH. In addition, the pathoplastic moderating effect was evaluated using ordered logistic regression with an interaction term of depressive mood and education in the regression model. All analyses adjusted for complex sample weights so that findings are nationally representative. Results: After adjusting for all covariates, U.S. cancer survivors’ depressive mood was significantly associated with lower SRH, and U.S. cancer survivors’ higher education was significantly associated with higher SRH. As a pathoplastic moderator, cancer survivors’ education significantly moderated the association between depressive mood and SRH. The negative association between depressive mood and SRH was significantly greater among those with higher education. Conclusion: Moving beyond the direct association between depressive mood, education, and SRH, education served as a pathoplastic moderator in relation to depressive mood and SRH. Psycho-oncology providers need to be mindful of the “protective-risk” effect of education in relation to cancer survivors’ depressive mood and SRH.
Bird Functional Diversity in Agroecosystems and Secondary Forests of the Tropical Andes
Authors: Velásquez-Trujillo Vanessa , Betancurt-Grisales Juan F. , Vargas-Daza Angela M. , Lara Carlos E. , Rivera-Páez Fredy A. , Fontúrbel Francisco E. , Castaño-Villa Gabriel J.
Published: October 2021

Agricultural systems have increased in extension and intensity worldwide, altering vertebrate functional diversity, ecosystem functioning, and ecosystemic services. However, the effects of open monoculture crops on bird functional diversity remain little explored, particularly in highly biodiverse regions such as the tropical Andes. We aim to assess the functional diversity differences of bird guilds between monoculture crops (coffee, cocoa, and citrus) and secondary forests. We use four functional diversity indices (Rao Q, Functional Richness, Functional Evenness, and Functional Divergence) related to relevant morphological, life history, and behavioral traits. We find significant differences in functional diversity between agroecosystem and forest habitats. Particularly, bird functional diversity is quite homogeneous among crop types. Functional traits related to locomotion (body weight, wing-chord length, and tail length), nest type (closed), and foraging strata (canopy and understory) are dominant at the agroecosystems. The bird assemblages found at the agroecosystems are more homogeneous in terms of functional diversity than those found at the secondary forests, as a result of crop structure and management. We recommend promoting more diverse agroecosystems to enhance bird functional diversity and reduce their effects on biodiversity.