bluuebunny/crossref_metadata_2025 huggingface dataset:
Supplementary Figure S2 from A B7-H4–Targeting Antibody–Drug Conjugate Shows Antitumor Activity in PARPi and Platinum-Resistant Cancers with B7-H4 Expression
Authors: Gitto Sarah B. , Whicker Margaret , Davies Gareth , Kumar Sushil , Kinneer Krista , Xu Haineng , Lewis Arthur , Mamidi Srinivas , Medvedev Sergey , Kim Hyoung , Anderton Judith , Tang E. Jessica , Ferman Benjamin , Coats Steven , Wilkinson Robert W. , Brown Eric , Powell Daniel J. , Simpkins Fiona
Published: April 2024

B7-H4 cell surface expression in cell lines and B7-H4-ADC cytotoxicity.
Supplementary Table 1 from Multi-omics Analysis Reveals Immune Features Associated with Immunotherapy Benefit in Patients with Squamous Cell Lung Cancer from Phase III Lung-MAP S1400I Trial
Authors: Parra Edwin Roger , Zhang Jiexin , Duose Dzifa Yawa , Gonzalez-Kozlova Edgar , Redman Mary W. , Chen Hong , Manyam Ganiraju C. , Kumar Gayatri , Zhang Jianhua , Song Xingzhi , Lazcano Rossana , Marques-Piubelli Mario L. , Laberiano-Fernandez Caddie , Rojas Frank , Zhang Baili , Taing Len , Jhaveri Aashna , Geisberg Jacob , Altreuter Jennifer , Michor Franziska , Provencher James , Yu Joyce , Cerami Ethan , Moravec Radim , Kannan Kasthuri , Luthra Rajyalakshmi , Alatrash Gheath , Huang Hsin-Hui , Xie Hui , Patel Manishkumar , Nie Kai , Harris Jocelyn , Argueta Kimberly , Lindsay James , Biswas Roshni , Van Nostrand Stephen , Kim-Schulze Seunghee , Gray Jhanelle E. , Herbst Roy S. , Wistuba Ignacio I. , Gettinger Scott , Kelly Karen , Bazhenova Lyudmila , Gnjatic Sacha , Lee J. Jack , Zhang Jianjun , Haymaker Cara
Published: April 2024

Supplementary Table 1. Clinicopathologic from total patients and according to assay.
Supplementary Figure 1 from Multi-omics Analysis Reveals Immune Features Associated with Immunotherapy Benefit in Patients with Squamous Cell Lung Cancer from Phase III Lung-MAP S1400I Trial
Authors: Parra Edwin Roger , Zhang Jiexin , Duose Dzifa Yawa , Gonzalez-Kozlova Edgar , Redman Mary W. , Chen Hong , Manyam Ganiraju C. , Kumar Gayatri , Zhang Jianhua , Song Xingzhi , Lazcano Rossana , Marques-Piubelli Mario L. , Laberiano-Fernandez Caddie , Rojas Frank , Zhang Baili , Taing Len , Jhaveri Aashna , Geisberg Jacob , Altreuter Jennifer , Michor Franziska , Provencher James , Yu Joyce , Cerami Ethan , Moravec Radim , Kannan Kasthuri , Luthra Rajyalakshmi , Alatrash Gheath , Huang Hsin-Hui , Xie Hui , Patel Manishkumar , Nie Kai , Harris Jocelyn , Argueta Kimberly , Lindsay James , Biswas Roshni , Van Nostrand Stephen , Kim-Schulze Seunghee , Gray Jhanelle E. , Herbst Roy S. , Wistuba Ignacio I. , Gettinger Scott , Kelly Karen , Bazhenova Lyudmila , Gnjatic Sacha , Lee J. Jack , Zhang Jianjun , Haymaker Cara
Published: April 2024

Supplementary Figure 1. Available tumor tissue samples and blood samples.
Abstract LT07: Signalling Inhibition by Ponatinib Disrupts Productive Alternative Lengthening of Telomeres (ALT)
Authors: Kusuma Frances , Prabhu Aishvaryaa , Tieo Galen , Ahmad Syed Moiz , Dakle Pushkar , Yong Wai Khang , Pathak Elina , Madan Vikas , Jiang Yan Yi , Tam Wai Leong , Kappei Dennis , Dröge Peter , Koeffler H. Phillip , Jeitany Maya
Published: April 2024

Enabling replicative immortality is one prominent feature of cancer cells. To that end, cancer cells activate means to elongate their telomeres. While 85% of cancers re-express telomerase, around 15% of all cancers rely on the ALT (Alternative Lengthening of Telomeres) mechanism. Despite its frequency, no clinically approved ALT-targeted therapy exists to date.

By performing anti-cancer compound library screen on isogenic cell lines and using extrachromosomal telomeric C-circles, as a bona fide marker of ALT activity, we identified a receptor tyrosine kinase inhibitor, ponatinib, that deregulates ALT mechanisms, induces telomeric dysfunction, reduced ALT- associated telomere synthesis, and targets, in vivo, ALT-positive cells.

By scrutinizing the mode of action of ponatinib on ALT, we identified an ABL1-JNK-JUN signalling circuit to be inhibited by ponatinib and to have a role in suppressing extrachromosomal telomeric C-circle formation. Furthermore, transcriptome and interactome analyses of JUN suggested a role of JUN in DNA damage repair pathways, independently of its capacity as a transcription factor. These results were corroborated by new synergistic drug interactions between ponatinib and either DNA synthesis or repair inhibitors such as triciribine and KU-60019, respectively. Overall, we identified a novel signalling pathway impacting ALT which can be targeted by a clinically approved kinase inhibitor.

Citation Format: Frances Kusuma, Aishvaryaa Prabhu, Galen Tieo, Syed Moiz Ahmad, Pushkar Dakle, Wai Khang Yong, Elina Pathak, Vikas Madan, Yan Yi Jiang, Wai Leong Tam, Dennis Kappei, Peter Dröge, H. Phillip Koeffler, Maya Jeitany. Signalling Inhibition by Ponatinib Disrupts Productive Alternative Lengthening of Telomeres (ALT) [abstract]. In: Proceedings of Frontiers in Cancer Science; 2023 Nov 6-8; Singapore. Philadelphia (PA): AACR; Cancer Res 2024;84(8_Suppl):Abstract nr LT07.
Abstract P05: Novel PROTAC-based EZH2 Degraders Effectively Target Lymphoid Malignancies by Depleting Enzyme-Independent Functions of EZH2
Authors: Mustafa Nurulhuda , Alnaseri Zubaida Talal , Jian Darrel Tan Egk , Truong David Quach Thanh , Lim Wan Hsin , Chng Wee Joo
Published: April 2024

Overexpression or mutation of the epigenetic silencer EZH2 correlates with invasive cancer growth, resistance, and poor survival outcomes. Previously, we have established a methylation-independent role for EZH2 in the transcriptional activation of oncogenes in NKTL. As EZH2 enzymatic inhibitors have proven ineffective against NKTL, we embarked on the development of novel EZH2 Proteolysis Targeting Chimera (PROTAC) compounds. EZH2 PROTACs consist of an EZH2- and E3-ligase binding domain which promotes targeted ubiquitination of the EZH2 protein thereby marking it for proteasomal degradation.

Our initial efforts focused on the iterative synthesis of EZH2 PROTAC candidates optimizing factors such as permeability, solubility and stable ternary complex formation. Through extensive screening, we identified C2911 and C1311, which successfully trigger the dose-dependent degradation of EZH2 and PRC2 components concomitant with a significant loss of survival and induction of apoptosis in both NKTL and DLBCL. These effects were rescued by pre-treatment with proteasome inhibitor Bortezomib or when E3 ligase activity was disrupted through siRNA knockdown. In NKTL, we show that EZH2 PROTAC treatment decreased JAK3 and phospho-STAT5 protein expression and identified the suppression of the JAK-STAT-MYC axis as a potential mechanism of action. EZH2 PROTAC treatment effectively reactivates transcription of canonical EZH2 target genes such as MYT1 and ANPEP and downregulates expression of oncogenes c-Myc and E2F1. Pharmacokinetic studies in vivo demonstrate that these PROTACs exhibit good plasma stability, half-life (>4h) and low clearance thereby establishing potential for future clinical development. The efficacy of EZH2 PROTACs in xenograft mice are currently being evaluated.

Collectively, our data supports the discovery of novel EZH2 PROTACs and the critical role it plays in successfully targeting the oncogenic role of EZH2 in NKTL or other non-canonical EZH2-driven cancers through protein depletion where enzymatic-only inhibition was ineffective. We are further investigating the mechanisms stabilizing EZH2 protein levels to enhance PROTAC-mediated cytotoxicity.

Citation Format: Nurulhuda Mustafa, Zubaida Talal Alnaseri, Darrel Tan Egk Jian, David Quach Thanh Truong, Wan Hsin Lim, Wee Joo Chng. Novel PROTAC-based EZH2 Degraders Effectively Target Lymphoid Malignancies by Depleting Enzyme-Independent Functions of EZH2 [abstract]. In: Proceedings of Frontiers in Cancer Science; 2023 Nov 6-8; Singapore. Philadelphia (PA): AACR; Cancer Res 2024;84(8_Suppl):Abstract nr P05.
Abstract P09: AN EX-VIVO COMBINATION DRUG SENSITIVITY PLATFORM FOR PREDICTING SENSITIVITY TO FLT3 INHIBITOR-BASED COMBINATION IN ACUTE MYELOID LEUKEMIA
Authors: Sahib Noor Rashidha Bte Meera , Ying Jen Wei , Ooi Melissa , Chee Yen Lin , Jayalakshmi  , Mohamed Jameelah Bte Sheik , Rashid Masturah , Chow Edward KH
Published: April 2024

FMS-like tyrosine kinase-3 (FLT3) mutations are often linked with poor prognosis in Acute myeloid leukemia (AML). Despite increasing availability of targeted therapy, such as FLT3 inhibitors (FLT3i), remission is often short-term and followed by relapse and drug resistance. With varying response rates to standard treatments, the need to understand the molecular mechanisms driving drug resistance and accurately predict responses to treatment is crucial. Here, we present the ability of Quadratic phenotypic optimization platform (QPOP), in accurately identifying potential responders to possible combination therapies in AML.

AML cell lines and AML patient samples were treated with a set panel of drugs including FLT3i. QPOP drug combination scores and ranking were derived from cell viability response following treatment. 40 patients were recruited for the study, of which 20 patients were evaluated for their clinical concordance. RNA sequencing and molecular analysis was further conducted to determine the accuracy of QPOP predicted response and to determine mechanism of resistance to FLT3i.

QPOP predicted response was concordant to patient treatment outcome with 80% sensitivity and specificity. QPOP analysis on AML cell lines accurately identified responders to FLT3i including the differential response between a FLT3- ITD isogenic parental and FLT3i resistant cell line. Serial QPOP analysis in a FLT3 mutant patient sample indicated a decrease in FLT3i sensitivity concordant with an increasing FLT3 allelic burden and the development of resistance. The development of resistance to FLT3i based combinations was correlated to an increase in phosphorylated AKT expression. Activation of AKT is associated with the upregulation of Forkhead box M1 (FoxM1) in the resistant cell line. Co-treatment with an AKT inhibitor restored sensitivity to FLT3i treatment in the resistant cells. We demonstrate that QPOP analysis accurately predicts sensitivity to drug-combinations and can be used as a tool to identify sensitivity to FLT3i-based combination therapy in AML.

Citation Format: Noor Rashidha Bte Meera Sahib, Jen Wei Ying, Melissa Ooi, Yen Lin Chee, Jayalakshmi, Jameelah Bte Sheik Mohamed, Masturah Rashid, Edward KH Chow. AN EX-VIVO COMBINATION DRUG SENSITIVITY PLATFORM FOR PREDICTING SENSITIVITY TO FLT3 INHIBITOR-BASED COMBINATION IN ACUTE MYELOID LEUKEMIA [abstract]. In: Proceedings of Frontiers in Cancer Science; 2023 Nov 6-8; Singapore. Philadelphia (PA): AACR; Cancer Res 2024;84(8_Suppl):Abstract nr P09.
Abstract P10: p44, the Amino-terminal Lacking Isoform of p53, is Dispensable for Tumor Suppression but Regulates Male Fertility
Authors: Chia RH , Phang Beng Hooi , Teo Fanny Xueting , Lau Hannah , Sabapathy Kanaga
Published: April 2024

p53 is the most studied tumor-suppressor, by virtue of its functional inactivation in most human cancers. One aspect that has not received sufficient attention is the function of the various forms of p53, such as p47, that lacks the amino-terminal transactivation domain. Hence, the goal of this work has been to systematically characterize p47, which was found to retain the ability to selective transactivate apoptotic target gene activation (and thus the ability to induce apoptosis), but lacks the ability to induce the expression of genes involved in cell cycle arrest. Consistently, patients with tumors that have p53 mutations that lead to p47 expression have a better prognosis. We have uncovered the mechanistic basis of the selective transcriptional activity of p47, which is associated with selective co-factor binding. In addition, we have generated mice that express only p47 (without p53), and show that they are susceptible to spontaneous tumors, like human patients who have germ-line mutations that lead to only p47 expression. Thus, p47 appears to be a null allele in mice with respect to tumor formation, and is incapable of inducing selective target gene activation nor apoptosis, highlighting species-specific differences in its functionality.

Further characterization of these mice will be reported.

Citation Format: RH Chia, Beng Hooi Phang, Fanny Xueting Teo, Hannah Lau, Kanaga Sabapathy. p44, the Amino-terminal Lacking Isoform of p53, is Dispensable for Tumor Suppression but Regulates Male Fertility [abstract]. In: Proceedings of Frontiers in Cancer Science; 2023 Nov 6-8; Singapore. Philadelphia (PA): AACR; Cancer Res 2024;84(8_Suppl):Abstract nr P10.
Wirksamkeit von Teprotumumab bei der chronischen endokrinen Orbitopathie
Authors: Bertelmann Eckart
Published: April 2024

Context: Early inflammatory thyroid eye disease (TED) can lead to symptomatic chronic disease including disabling proptosis. Teprotumumab, an IGF-1 receptor inhibitor, previously demonstrated efficacy in acute, high-inflammation TED trials. Objective: We present data from the first placebo-controlled trial with teprotumumab in chronic/low disease activity TED. Design: This was a randomized double-masked, placebo-controlled trial. Setting: The study was conducted in 11 US centers. Participants: Adults with TED duration 2–10 years, Clinical Activity Score (CAS) ≤ 1 or no additional inflammation or progression in proptosis/diplopia for ≥ 1 year, proptosis ≥ 3 mm from before TED/from normal, euthyroid/mildly hypo/hyperthyroid, no prior teprotumumab, and no steroids within 3 weeks of baseline participated. Intervention: Patients received (2:1) intravenous teprotumumab or placebo once every 3 weeks (total 8 infusions). Main outcome measures: The primary endpoint was proptosis (millimeter) improvement at Week-24. Adverse events (AEs) were assessed. Results: 42 teprotumumab and 20 placebo patients were randomized. At Week-24, mean (SD) proptosis improvement was greater with teprotumumab (-2.41 [0.228]) than placebo (-0.92 [0.323]), difference -1.48, 95%CI -2.28, -0.69, P = .0004. Proportions of patients with AEs were similar between groups. Hyperglycemia was reported in 6(15%) vs 2(10%) and hearing impairment in 9(22%) vs 2(10%) with teprotumumab and placebo respectively. AEs led to discontinuation in one teprotumumab (left ear conductive hearing loss with congenital anomaly) and one placebo patient (infusion-related). There were no deaths.
La escuela y la familia en relación con el alcance del logro académico. La experiencia de la Institución Educativa Antonio José de Sucre de Itagüí (Antioquia) 2015
Authors: Villalobos Martínez José Luis , Flórez Romero Gabriel Antonio , Londoño Vásquez David Alberto
Published: April 2024

En el presente artículo, se analizan los resultados de las narrativas aportadas por estudiantes, madres de familia y docentes frente a la importancia del acompañamiento de la familia en los procesos de formación que se imparten en la escuela como oportunidad para alcanzar el logro académico en el grado undécimo de la Institución Educativa Antonio José de Sucre de Itagüí en el año 2015. Dichas narrativas permitieron comprender el desarrollo de las dinámicas familiares como una oportunidad para afianzar los procesos de formación que se imparten desde la escuela. Con relación a los hallazgos, estos nos llevaron a concluir que, sin la complementariedad entre la escuela y la familia, los procesos académicos podrían generar frustración en los estudiantes. Por tanto, los resultados de esta investigación se convierten en una herramienta para repensar la relación de la labor educativa entre familia y escuela.
Atlas escorpiônico de São Paulo: distribuição espacial e temporal
Authors:   Universidade de São Paulo. Faculdade de Saúde Pública da USP, Ferraz Sergio Cominato , Chiaravalloti Neto Francisco ,   Universidade de São Paulo. Faculdade de Saúde Pública
Published: April 2024

Para demonstrar a situação da distribuição dos escorpiões e acidentes escorpiônicos entre 2013 e 2018 em São Paulo, produzimos este livro com mapas e gráficos baseados nos dados de notificações de escorpiões coletados nos serviços prestados pela prefeitura de São Paulo à população e nos dados do Sinan. Este livro visa dar suporte a atividades realizadas pelas equipes de vigilância em saúde de toda a cidade; mostrando uma visão detalhada do escorpionismo para os diversos segmentos de saúde, ciência e órgãos de governo de todo o país; assim como para o mundo. Ele auxilia no desenvolvimento da prevenção ao agravo, fornecendo material para o desenvolvimento da ciência e apoiando a organização do sistema de atenção à saúde. A apresentação das figuras do atlas está organizada conforme a relação de conteúdo, buscando facilitar a visualização, seguindo a hierarquia das divisões administrativas em saúde; levando em consideração fatores como: espécies de importância médica, interações do fenômeno no espaço e no tempo, capacidade de locomoção dos escorpiões, capacidade de dispersão, nível de infestação e risco de acidentes, necessidade de prevenção do escorpionismo ou atenção aos acidentados entre outros fatores. Mapas de calor também são apresentados para descrever o fenômeno.
Contradições da cidade compacta: reflexões críticas a partir das lógicas contemporâneas de produção do espaço em São Paulo
Authors: Lima Bruno Avellar Alves de
Published: April 2024

Diante da crise ecológica e climática mundial, apresentam-se reiteradamente, no âmbito internacional, políticas voltadas à promoção de cidades compactas. Estas, segundo tais políticas, trariam ganhos socioambientais, como a diminuição das emissões de gases do efeito estufa pela redução nos deslocamentos motorizados. Tais consensos, no entanto, acabam por obscurecer as lógicas de produção do espaço e os interesses contraditórios que envolvem o adensamento, sobretudo em termos de intensificação da produção imobiliária, a qual não se encontra diretamente associada à democratização de territórios dotados de infraestrutura adequada. A partir do quadro exposto, o texto problematiza a concepção dominante de cidade compacta sob a perspectiva das lógicas contemporâneas de produção do espaço, iluminando as contradições que envolvem a centralidade do imobiliário em um contexto neoliberal. Adotamos como perspectiva uma abordagem dialética, buscando apreender a realidade a partir da análise de suas contradições. Além de revisão da literatura e documental, o caso de São Paulo é mobilizado como experiência empírica, na qual a adoção de políticas de adensamento se mostra insuficiente na resolução dos problemas socioambientais.
Multimodalidade e cenografia em quatro edições da Antologia da Literatura Fantástica
Authors: Primo Gustavo
Published: April 2024

A partir das perspectivas da análise do discurso literário proposta por Maingueneau (2006) e dos estudos das modalidades da mídia, discutidos por Elleström (2010), analisaremos quatro edições, publicadas em tempos e lugares diversos, da Antologia da Literatura Fantástica, originalmente organizada por Silvina Ocampo, Adolfo Bioy Casares e Jorge Luis Borges. Observa-se que a cada vez que a Antologia foi (re)publicada por diferentes casas editoriais, a obra ganhou uma materialização diferente, em diferentes objetos-livros, cada um com suas especificidades textuais, paratextuais, gráficas, materiais. Todos esses aspectos se nos revelam como vestígios de modos de funcionamento do discurso literário e é possível, portanto, interpretar cada livro como possuindo uma cenografia reveladora de imaginários editoriais acerca do que seja a literatura fantástica, como ela deva circular e qual seja seu valor simbólico. Discutiremos a cenografia articulada pela materialização de quatro edições diferentes da Antologia: a primeira, publicada pela Editorial Sudamericana (Argentina, 1940); uma edição da Xanadu Publications (Reino Unido, 1988); uma da Debolsillo (Argentina, 2016) e uma da Cosac Naify (Brasil, 2014). Cada uma delas nos revela como o texto literário não é puramente fruto de um autor único, mas de uma miríade de mediadores editoriais (editores, diagramadores, capistas, designers, prefaciadores etc.).
Psihološki aspekti razodjevenosti u medicinskim postupcima
Authors: Ješić Valentina , Adamović Antonija , Babić Sabina , Vratan Nikolina
Published: April 2024

Uvod: U suvremenoj medicini razodjevenost, odnosno izlaganje tijela tijekom operacijskih zahvata postaje ključna tema izazivajući kompleksne emocionalne odgovore kod pacijenata. Emocionalni aspekti povezani s privatnošću, dostojanstvom i autonomijom zahtijevaju dublje razumijevanje da bi se oblikovale prakse koje osiguravaju medicinsku učinkovitost i emocionalno blagostanje pacijenata. Metode: Istraživanje je provedeno putem online ankete kako bi se prikupila iskustva osoba koje su imale operacijske zahvate. Sudjelovalo je ukupno 200 ispitanika, od kojih je 141 žena i 59 muškaraca, a istraživanje je obuhvatilo pitanja o emocionalnom stanju, privatnosti, osjećaju srama, poštovanju digniteta te stavovima o pripremi i komunikaciji s medicinskim osobljem. Rezultati: Rezultati istraživanja o emocionalnom stanju pacijenata prije operacijskih zahvata pokazuju da 58 % ispitanika izražava zabrinutost, 24 % njih osjeća sigurnost, dok 11 % ispitanika pokazuje ravnodušnost. Ukupno 55 % ispitanika doživljava visok stupanj srama tijekom priprema za operacijski zahvat. Nakon provedbe zahvata, 50 % pacijenata izražava osjećaj srama, 24 % osjeća anksioznost, dok 7 % njih doživljava olakšanje. Procjena privatnosti tijekom priprema pokazuje da 55 % pacijenata doživljava nisku razinu privatnosti. Pacijenti ocjenjuju komunikaciju o privatnosti prije operacijskog zahvata različito, pri čemu 28 % njih smatra da je jasna, dok 35 % ispitanika nije percipiralo komunikaciju o privatnosti. Glavni prijedlozi pacijenata za poboljšanje privatnosti uključuju veću upotrebu paravana (32 %) te dodatnu edukaciju medicinskog osoblja u segmentu održavanja privatnosti (28 %). Zaključak: Istraživanje ukazuje na važnost socijalne podrške te dosljednog informiranja pacijenata kako bi se poboljšala njihova priprema i razumijevanje emotivnih izazova. Navedeni koraci predstavljaju temelje za prilagodbu medicinskih praksi s ciljem očuvanja pacijentova emocionalnog blagostanja, čime se istovremeno jača povjerenje u medicinsko osoblje i sigurnost u procesu liječenja.
Uloga medicinske sestre u edukaciji djeteta oboljelog od astme
Authors: Šoprun Renata , Munivrana Škvorc Helena , Vučemilović Ivana , Jerković Ivana , Škvorc Marko
Published: April 2024

Uvod: Astma je najčešća kronična bolest kod djece. Karakteriziraju je reverzibilna opstrukcija dišnih putova i povećana bronhalna hiperaktivnost koje su uzrokovane kroničnom upalom potaknutom čimbenicima iz okoliša. Glavni su simptomi sipnja, kašalj, otežano disanje i stezanje u prsima. U liječenju astme kod djece primjenjuju se međunarodno prihvaćene GINA smjernice. Astma se ne može izliječiti, ali se može kontrolirati. Kontrolom bolesti postiže se stanje u kojem dijete uz minimalnu terapiju nema simptome. Kad astma nije kontrolirana, dolazi do egzacerbacije i pojave simptoma koji mogu biti blagi do životno ugrožavajući. U liječenju se upotrebljavaju lijekovi koji kontroliraju upalu i/ili simptomatski lijekovi. Za postizanje kontrole bolesti važan je individualni pristup, redovite kontrole i edukacija. Medicinska sestra ima vrlo važnu ulogu u edukaciji djece koja se može provoditi individualno ili grupno u Astma školi. Cilj: Cilj je ispitati razliku između individualnog i grupnog načina edukacije o astmi te razlike u usvojenom znanju prema dobi i spolu djece. Rezultati: U istraživanju su sudjelovala 83 ispitanika u dobi od 5 do 18 godina oba spola koji boluju od astme. Podaci su prikupljeni ispunjavanjem ankete koja se sastoji od 20 pitanja. Istraživanje je provedeno prilikom kontrolnog pregleda djece u specijalističkoj pulmološkoj i alergološkoj ambulanti u Dječjoj bolnici Srebrnjak u Zagrebu. Dobiveni rezultati pokazuju da nema značajne razlike u kontroli bolesti astme prema spolu i dobi kad je primijenjena individualna i kad je primijenjena grupna edukacija. Zaključak: Povećanjem broja edukacija, kao i suvremenijim pristupom edukaciji pomoću računalne tehnologije, putem online predavanja, radionica ili korištenjem tzv. ozbiljnih igara, mogla bi se povećati kvaliteta edukacije i interes za edukaciju o astmi.
Atrial Fibrillation Screening During Routine Automated Office, Home, and Ambulatory Blood Pressure Measurement: A Diagnostic Test Accuracy Systematic Review and Meta-Analysis
Authors: Kyriakoulis Konstantinos G. , Kollias Anastasios , Menti Ariadni , Chardouvelis Panagiotis , Stergiou George S.
Published: April 2024

BACKGROUND:

Atrial fibrillation (AF) is often asymptomatic and undiagnosed. As AF and hypertension often coexist, opportunistic AF detection during routine automated blood pressure (BP) measurement appears to be an attractive screening method.

METHODS:

A systematic literature search was conducted to identify studies assessing the diagnostic test accuracy of office, home, or 24-hour ambulatory BP measuring devices with AF detection algorithms versus reference electrocardiography. Analyses were performed per participant (AF status based on several BP readings; most office/home devices) or per reading (AF status based on individual readings; all ambulatory devices). A meta-analysis stratified by device type (office/home/ambulatory) was conducted to calculate pooled measures of diagnostic accuracy. Sensitivity/meta-regression analyses were also performed.

RESULTS:

Among 3096 records initially retrieved, 23 diagnostic test accuracy studies were included. Data derived from 11 093 individuals (weighted age 69 years, males 56%, hypertensives 79%, diabetics 24%, and AF prevalence 17%) indicated a pooled sensitivity 0.97 (95% CI, 0.92–0.99), specificity 0.93 (95% CI, 0.90–0.95), and accuracy 0.93 (95% CI, 0.89–0.95), with generally consistent results using office, home, or ambulatory BP devices (slightly lower specificity with the latter). The positive and negative predictive values were 0.70 (95% CI, 0.60–0.80) and 0.99 (95% CI, 0.98–1.00), respectively. Sensitivity analyses indicated lower specificity in studies implementing reading versus participant analyses. Most studies presented a low risk of bias and minor applicability concerns.

CONCLUSIONS:

There is considerable and consistent evidence suggesting high diagnostic accuracy of AF detection algorithms implemented in automated BP monitors during routine BP measurements in and out of the office. AF diagnosis requires verification (electrocardiography) before treatment is administered.
Personality Traits of Freelancers Working Remotely
Authors: Rodina Olga N. , Prudkov Pavel N.
Published: April 2024

Background. Remote work has been gaining popularity for a long time. With the outbreak of the COVID-19 pandemic, remote work has become a necessity for many people. However, the personality traits of people for whom remote work is suitable are poorly understood. Objectives. The study focuses on the personality traits of successful freelancers working remotely. Study Participants. 184 Freelancers (115 females, 69 males, aged from 14 to 60) were recruited from Advego.com. Freelancers were divided into two groups of beginners and experts, based on the number of tasks completed. Groups were compared with each other. The group of experts was also compared with a group of 98 females (aged from 16 to 72) who were not freelancers. Methods. An empirical study was carried out using a five-factor personality questionnaire by H. Tsuji in the version of A.B. Khromov. Results. Significant differences were found between expert freelancers and women of different professions for the following personality traits: extraversion (p = 0.0001), agreeableness (p = 0.0001), openness to experience (p = 0.0001). The hypothesis that freelancers have a higher level of conscientiousness than non-freelance women was not confirmed. Conclusions. The personality traits of freelancers who are successfully adapted to remote work are revealed including lower levels of extraversion, agreeableness, and openness to experience. The results obtained can be used to select staff for remote work.
Current Directions of Entrepreneurship Research in Social Psychology: a Map of the Scientific Landscape
Authors: Cheremenskaia Maria A.
Published: April 2024

Background. Currently, the number of entrepreneurs who want to run a traditional business or a startup is growing rapidly. In scientific circles, there is also increasing interest in the phenomenon of entrepreneurship and, in particular, in the personality of the entrepreneur. The question arises as to which areas of research in the social psychology of entrepreneurship are relevant at the moment. Objective. The study is aimed at creating a map of scientific landscape in the field of entrepreneurship, as well as at highlighting relevant areas of entrepreneurship research in social psychology. Methods. Scientific mapping was carried out using the VOSviewer programme version 1.6.15. A scientometric analysis of keywords from 3,235 scientific articles published in the field of social psychology over the past 5 years was carried out (according to The Lens platform). The interpretation of the map in order to highlight the current directions was made using the metaphor of the city. Results. Current research topics include personal and environmental aspects. Among the relevant areas of entrepreneur's research are socio-psychological personality traits (optimism, enthusiasm, perseverance, traits of the dark triad, etc.), entrepreneurial intentions, motivation. When studying entrepreneurship, the question of the choice to become an entrepreneur as well as the process of entrepreneurial self-determination are important. At the same time, at this stage of research, the thematic bundle “identity-self-determination-intentions” is of interest only from the point of view of intentions. Conclusions. Entrepreneurship as a phenomenon in modern social psychology is of scientific interest in terms of point constructs: entrepreneurial intentions, socio-psychological personality traits, values. It is the study of entrepreneurial identity that seems promising, since it will allow to predict the professional path of young people and their predisposition to entrepreneurship.
Nepenthes kampalili (Nepenthaceae), a new species of pitcher plant from the eastern mindanao biodiversity corridor, Philippines
Authors: LAGUNDAY NOEL E. , YURONG AIMANUELZON , AMOROSO VICTOR B.
Published: April 2024

A new species, restricted in the limestone karst bonsai forest of the eastern Mindanao biodiversity corridor, Nepenthes kampalili grouped under section Villosa is herein described with a taxonomic key leading to the species. It differs from N. peltata in having apically attached tendril to leaf (vs. peltate tendril-leaf attachment), densely pubescent ground pitchers (vs. glabrous), densely pubescent lid dorsal surface (vs. glabrous), and occasionally has 2 pitcher spurs arising from the midsection of the peristome neck posterior (vs. one unbranched pitcher spur). The species described is assessed as Critically Endangered facing threats of poaching and habitat loss without legislative protection and calls for immediate conservation efforts. Further, due to a lack of description for the rosette stems bearing ground pitchers and male inflorescence of N. candalaga the latter plant parts are described herein.
New records of earthworms (Clitellata: Oligochaeta) from Georgia, USA, including eight new state records
Authors: MCGEE JOSEPH M. , CARRERA-MARTÍNEZ ROBERTO , JR. MAC A. CALLAHAM , SNYDER BRUCE A.
Published: April 2024

We present here new county records for 13 counties within Georgia, USA from the collections of the Georgia Museum of Natural History and the authors. These include four families, eight genera, and 23 species. Of these, eight species are new state records: Amynthas carnosus (Goto & Hatai, 1899); Aporrectodea longa (Ude, 1885); Bimastos parvus (Eisen, 1874); Diplocardia deborahae Damoff & Reynolds, 2017; Diplocardia gatesi Murchie, 1965; Diplocardia michaelseni Eisen, 1899; Metaphire hilgendorfi (Michaelsen, 1892); and Murchieona minuscula (Rosa, 1906). These findings are presented alongside an updated checklist for Middle Georgia building on data compiled by Reynolds (2015) to create a practical record for the region.
Healthcare Access Worsened for Women in Precarious Housing During the COVID-19 Pandemic: A Qualitative Study
Authors: Sevik Irem , Ciceklioglu Meltem
Published: April 2024

This research aims to gain an in-depth understanding of precariously housed women’s experiences related to health and access to health care during the COVID-19 pandemic using a grounded theory approach. Qualitative data were obtained through interviews with 17 precariously housed women from Izmir, Turkey. Poor health among most participants was primarily attributed to unfavorable living conditions and weakened community networks. The COVID-19 pandemic exacerbated existing health issues due to barriers in accessing basic needs. Food insecurity was widespread during the pandemic and the critical role of aid and the inadequacy of social assistance in securing food were emphasized. Women’s health perceptions were significantly shaped by gender, and gendered caregiving duties have restricted women’s healthcare access. Access to healthcare was also limited by financial challenges, with health insurance being a crucial determinant. Longer waiting times, often exacerbated by the appointment system, and language were significant barriers to healthcare access. The findings propose that the participants were precarized by the blindness of COVID-19 measures to vulnerabilities, which resulted in deeper inequalities in housing, food, employment, and healthcare access. This research addresses the political, commercial, and social determinants of precariously housed women’s health. Improving precariously housed women’s health and wellbeing requires implementation of public policies targeting to improve housing quality, provide targeted assistance to food insecurity, promote gender inclusiveness, and foster gender empowerment.
Neuroprotective effects of lactate and ketone bodies in acute brain injury
Authors: Plourde Guillaume , Roumes Hélène , Suissa Laurent , Hirt Lorenz , Doche Émilie , Pellerin Luc , Bouzier-Sore Anne-Karine , Quintard Hervé
Published: April 2024

The goal of neurocritical care is to prevent and reverse the pathologic cascades of secondary brain injury by optimizing cerebral blood flow, oxygen supply and substrate delivery. While glucose is an essential energetic substrate for the brain, we frequently observe a strong decrease in glucose delivery and/or a glucose metabolic dysregulation following acute brain injury. In parallel, during the last decades, lactate and ketone bodies have been identified as potential alternative fuels to provide energy to the brain, both under physiological conditions and in case of glucose shortage. They are now viewed as integral parts of brain metabolism. In addition to their energetic role, experimental evidence also supports their neuroprotective properties after acute brain injury, regulating in particular intracranial pressure control, decreasing ischemic volume, and leading to an improvement in cognitive functions as well as survival. In this review, we present preclinical and clinical evidence exploring the mechanisms underlying their neuroprotective effects and identify research priorities for promoting lactate and ketone bodies use in brain injury.
A review of extant literature and recent trends in residential construction waste reduction
Authors: Albsoul Hadeel , Doan Dat Tien , Aigwi Itohan Esther , GhaffarianHoseini Ali
Published: April 2024

The residential construction sector in New Zealand and worldwide is experiencing increased criticism for generating substantial waste that poses environmental concerns. Accordingly, researchers have advocated implementing residential construction waste reduction (RCWR) strategies as a sustainable solution to managing construction waste (CW). This article aims to provide a comprehensive overview of RCWR by analysing 87 articles from the Scopus database using bibliometric and critical review methods. The co-occurrence analysis of keywords revealed five clusters, in which five main themes emerged: (i) waste generation and management performance, (ii) prefabrication and life cycle assessment concepts, (iii) design concepts, (iv) circular economy and (v) decision-making concepts. The findings suggest that sustainable practices such as designing for waste reduction, prefabrication, waste quantification, three-dimensional printing and building information modelling can effectively achieve RCWR. The study also highlights the benefits of RCWR, including reducing environmental impacts, and identifies management, economic, legislative, technology and cultural barriers that affect the implementation of RCWR strategies. These results provide valuable insights to support future policy formulation and research direction for RCWR in New Zealand.
Increased Anti-Inflammatory Therapeutic Potential and Progenitor Marker Expression of Corneal Mesenchymal Stem Cells Cultured in an Optimized Propagation Medium
Authors: Hopkinson Andrew , Notara Maria , Cursiefen Claus , Sidney Laura E.
Published: April 2024

There is a huge unmet need for new treatment modalities for ocular surface inflammatory disorders (OSIDs) such as dry eye disease and meibomian gland dysfunction. Mesenchymal stem cell therapies may hold the answer due to their potent immunomodulatory properties, low immunogenicity, and ability to modulate both the innate and adaptive immune response. MSC-like cells that can be isolated from the corneal stroma (C-MSCs) offer a potential new treatment strategy; however, an optimized culture medium needs to be developed to produce the ideal phenotype for use in a cell therapy to treat OSIDs. The effects of in vitro expansion of human C-MSC in a medium of M199 containing fetal bovine serum (FBS) was compared to a stem cell medium (SCM) containing knockout serum replacement (KSR) with basic fibroblast growth factor (bFGF) and human leukemia inhibitory factor (LIF), investigating viability, protein, and gene expression. Isolating populations expressing CD34 or using siRNA knockdown of CD34 were investigated. Finally, the potential of C-MSC as a cell therapy was assessed using co-culture with an in vitro corneal epithelial cell injury model and the angiogenic effects of C-MSC conditioned medium were evaluated with blood and lymph endothelial cells. Both media supported proliferation of C-MSC, with SCM increasing expression of CD34, ABCG2, PAX6, NANOG, REX1, SOX2, and THY1, supported by increased associated protein expression. Isolating cell populations expressing CD34 protein made little difference to gene expression, however, knockdown of the CD34 gene led to decreased expression of progenitor genes. C-MSC increased viability of injured corneal epithelial cells whilst decreasing levels of cytotoxicity and interleukins-6 and -8. No pro-angiogenic effect of C-MSC was seen. Culture medium can significantly influence C-MSC phenotype and culture in SCM produced a cell phenotype more suitable for further consideration as an anti-inflammatory cell therapy. C-MSC show considerable potential for development as therapies for OSIDs, acting through anti-inflammatory action.
Optimization of Xenografting Methods for Generating Human Skeletal Muscle in Mice
Authors: O’Neill Andrea , Martinez Anna Llach , Mueller Amber L. , Huang Weiliang , Accorsi Anthony , Kane Maureen A. , Eyerman David , Bloch Robert J.
Published: April 2024

Xenografts of human skeletal muscle generated in mice can be used to study muscle pathology and to test drugs designed to treat myopathies and muscular dystrophies for their efficacy and specificity in human tissue. We previously developed methods to generate mature human skeletal muscles in immunocompromised mice starting with human myogenic precursor cells (hMPCs) from healthy individuals and individuals with facioscapulohumeral muscular dystrophy (FSHD). Here, we examine a series of alternative treatments at each stage in order to optimize engraftment. We show that (i) X-irradiation at 25Gy is optimal in preventing regeneration of murine muscle while supporting robust engraftment and the formation of human fibers without significant murine contamination; (ii) hMPC lines differ in their capacity to engraft; (iii) some hMPC lines yield grafts that respond better to intermittent neuromuscular electrical stimulation (iNMES) than others; (iv) some lines engraft better in male than in female mice; (v) coinjection of hMPCs with laminin, gelatin, Matrigel, or Growdex does not improve engraftment; (vi) BaCl2 is an acceptable replacement for cardiotoxin, but other snake venom preparations and toxins, including the major component of cardiotoxin, cytotoxin 5, are not; and (vii) generating grafts in both hindlimbs followed by iNMES of each limb yields more robust grafts than housing mice in cages with running wheels. Our results suggest that replacing cardiotoxin with BaCl2 and engrafting both tibialis anterior muscles generates robust grafts of adult human muscle tissue in mice.
Protective Effect of Prunella vulgaris L. Whole Plant or Spicas Water Extracts on Hyperthyroidism with Liver-yang Hyperactivity Syndrome
Authors: Li Shuang , Gong Zhiguo , Hu Xiangling , Li Yufan , Yan Chao , Cao Liping , Chen Yuhang
Published: April 2024

Background

Prunella vulgaris L. (Lamiaceae) is a perennial medicinal plant commonly used to treat thyroid dysfunction, infectious hepatitis, and mastitis in north-east Asia and Europe. In China, there has been a historical change in the medicinal use of P. vulgaris from the use of whole plants to the use of the spicas.

Purpose

This study aimed to evaluate the protective effects of whole plant P. vulgaris water extracts and spicas on hyperthyroidism with liver-yang hyperactivity syndrome and to preliminarily explain the mechanism of action of these water extracts.

Materials and Methods

Rats were induced with aconite decoction and levothyroxine and treated with P. vulgaris whole plant and spicas water extract for 28 days at the same time. Body weight, food intake, and rectal temperature were monitored. The serum thyroid function indicators thyroid stimulating hormone (TSH), thyroid hormone-free triiodothyronine (FT3) and free thyroxine (FT4), liver function index alanine aminotransferase (ALT) and aspartate aminotransferase (AST), oxidative damage index malondialdehyde (MDA), and superoxide dismutase (SOD) were detected. Liver and thyroid tissues were taken for sectioning and observation. The liver inflammatory factors human tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) were detected by immunohistochemistry, and the expression levels of the apoptotic proteins caspase-3 and B-cell lymphoma-2 (Bcl-2) were detected.

Results

Both P. vulgaris whole plant and spica water extract groups exhibited markedly reduced FT3 and FT4 contents, increased the TSH content, and decreased ALT and AST activities. Furthermore, these agents improve thyroid and liver lesion tissue; reduce MDA content; increase SOD activity; reduce IL-6, TNF-α, and caspase-3 expression; and increase Bcl-2 expression.

Conclusion

The whole plant and spicas water extracts of P. vulgaris have obvious protective effects on hyperthyroidism and hyperactivity in liver yang, and the protective effect of the water extract of spicas is greater than that of the whole plant water extract.
Phytochemical and Anti-inflammatory Activities of Oxytropis aciphylla
Authors: Qian Hao , Liu Jiaqiong , Meng Xianqi , Wang Xiaoqin
Published: April 2024

Background

The objective of this research is to explore the phytochemical constituents and the potential anti-inflammatory properties of the herb Oxytropis aciphylla.

Materials and Methods

From the ethyl acetate and n-butanol fractions derived from the 70% ethanol extract of O. aciphylla, a total of 17 distinct chemical entities were extracted and subsequently identified. Utilizing network pharmacology, the study predicted the possible anti-inflammatory targets and mechanisms of action of O. aciphylla. The study assessed the anti-inflammatory impact of O. aciphylla extract on RAW 264.7 (a murine macrophage cell line) cells induced with lipopolysaccharide (LPS) by quantifying the concentrations of nitric oxide (NO), tumor necrosis factor-alpha (TNF- α), and interleukin-6 (IL-6).

Results

The isolated compounds included 12 flavonoids, two triterpenoids, one fatty acid, and two sterols. These specifically comprise apigenin, luteolin, kaempferol, isorhamnetin, naringenin, eriodictyol, dihydrokaempferol, butein, 7,4’-homoisoflavanone, apigenin-7- O- β-D-glucopyranoside, isorhamnetin-3- O- β-D-gentiobioside, rutin, azukisapogenol, 3- O-[ β-D-glucopyranosyl(1↓2)- β-D-glucuronopyranosyl] azukisapogenol methyl ester, tetracosanoic acid, daucosterol, and β-sitosterol. Network pharmacology analysis suggested that O. aciphylla may exert its anti-inflammatory effects through multiple components, targets, and pathways, such as kaempferol, apigenin, luteolin, AKT1, SRC, TNF, PTGS2, EGFR, MAPK, PI3K-Akt, VEGF, HIF-1, and TNF signaling pathways. The extract of O. aciphylla was found to markedly reduce the levels of NO, TNF- α, and IL-6 in RAW 264.7 cells that were stimulated with LPS, demonstrating a correlation with the dosage administered.

Conclusion

The investigation uncovered, for the first time, the phytochemical constituents and the mechanisms underlying the anti-inflammatory effects of O. aciphylla. These findings lay the groundwork for the scientific development and application of this botanical species as a natural remedy for inflammation.
Asymmetric Fiscal Federalism and Public Provision of Healthcare: New Insights from India
Authors: Harikrishnan K. S. , Hiremath Gourishankar S.
Published: April 2024

In this article, we show undesirable consequences of a market-based system and argue for a public provision of healthcare. We examine the healthcare provision from the fiscal federalism perspective. With the asymmetric fiscal federal structure characterised by vertical and horizontal imbalances, states are dependent on transfers from union to provide public goods. Our analysis shows a widespread disparity in the public provision of healthcare in India due to fiscal imbalances. The performance of states in the management of the COVID-19 pandemic reveals that a robust public healthcare system is a prerequisite in managing a health emergency. We identify the withdrawal of governments from healthcare provision and concentration of resources with the union government as critical impediments to achieving universal healthcare access. In light of our analysis, we emphasise the need for resource devolution and cooperative federalism to ensure public provision for a dignified life in a functioning democracy.
‘Oh my god this is happening’: how Our Flag Means Death staged an empathic mutiny against the labour of queer reading practices
Authors: Luttrell Briony , Banks Hannah Joyce
Published: April 2024

Our Flag Means Death ( OFMD) premiered to critical acclaim and unprecedented audience engagement. It can be argued that it is a romantic queer reading of historical facts. In this article, we reflect on the social function of storytelling and audience labour within the context of queer screen representations. We theorise queer reading as a practice of learning to recognise, identify and create patterns of semiotic resources. This practice is a reaction to a history of being erased or relegated to subtext. We argue queer reading is a particular form of audience labour, in that readers are asked to do extra work. This is especially important in cases where identities and communities are regularly symbolically annihilated. Season One of OFMD is a unique case study where we explore how the show achieves a low/easy labour environment for a vulnerable viewer and how this is an act of care and empathy.