bluuebunny/crossref_metadata_2025 huggingface dataset:
Novel LncRNA OXCT1-AS1 indicates poor prognosis and contributes to tumorigenesis by regulating miR-195/CDC25A axis in glioblastoma
Authors: Zhong Chen , Yu Qian , Peng Yucong , Zhou Shengjun , Liu Zhendong , Deng Yong , Guo Leiguang , Zhao Shiguang , Chen Gao
Published: April 2021

Background

Long noncoding RNAs (lncRNAs) contribute to multiple biological processes in human glioblastoma (GBM). However, identifying a specific lncRNA target remains a challenge. In this study, bioinformatics methods and competing endogenous RNA (ceRNA) network regulatory rules were used to identify GBM-related lncRNAs and revealed that OXCT1 antisense RNA 1 (OXCT1-AS1) is a potential therapeutic target for the treatment of glioma.

Methods

Based on the Gene Expression Omnibus (GEO) dataset, we identified differential lncRNAs, microRNAs and mRNAs and constructed an lncRNA-associated ceRNA network.

The novel lncRNA OXCT1-AS1 was proposed to function as a ceRNA, and its potential target miRNAs were predicted through the database LncBase Predicted v.2. The expression patterns of OXCT1-AS1 in glioma and normal tissue samples were measured. The effect of OXCT1-AS1 on glioma cells was checked using the Cell Counting Kit 8 assay, cell colony formation assay, Transwell assay and flow cytometry in vitro. The dual-luciferase activity assay was performed to investigate the potential mechanism of the ceRNA network. Finally, orthotopic mouse models of glioma were created to evaluate the influence of OXCT1-AS1 on tumour growth in vivo.

Results

In this study, it was found that the expression of lncRNA OXCT1-AS1 was upregulated in both The Cancer Genome Atlas (TCGA) GBM patients and GBM tissue samples, and high expression of OXCT1-AS1 predicted a poor prognosis. Suppressing OXCT1-AS1 expression significantly decreased GBM cell proliferation and inhibited cell migration and invasion. We further investigated the potential mechanism and found that OXCT1-AS1 may act as a ceRNA of miR-195 to enhance CDC25A expression and promote glioma cell progression. Finally, knocking down OXCT1-AS1 notably attenuated the severity of glioma in vivo.

Conclusion

OXCT1-AS1 inhibits glioma progression by regulating the miR-195-5p/CDC25A axis and is a specific tumour marker and a novel potential therapeutic target for glioma treatment.
Efficacy and mechanism of the combination of PARP and CDK4/6 inhibitors in the treatment of triple-negative breast cancer
Authors: Zhu Xiuzhi , Chen Li , Huang Binhao , Li Xiaoguang , Yang Liu , Hu Xin , Jiang Yizhou , Shao Zhimin , Wang Zhonghua
Published: April 2021

Background

PARP inhibitors (PARPi) benefit only a fraction of breast cancer patients with BRCA mutations, and their efficacy is even more limited in triple-negative breast cancer (TNBC) due to clinical primary and acquired resistance. Here, we found that the efficacy of the PARPi olaparib in TNBC can be improved by combination with the CDK4/6 inhibitor (CDK4/6i) palbociclib.

Methods

We screened primary olaparib-sensitive and olaparib-resistant cell lines from existing BRCAmut/TNBC cell lines and generated cells with acquired olaparib resistance by gradually increasing the concentration. The effects of the PARPi olaparib and the CDK4/6i palbociclib on BRCAmut/TNBC cell lines were examined in both sensitive and resistant cells in vitro and in vivo. Pathway and gene alterations were assessed mechanistically and pharmacologically.

Results

We demonstrated for the first time that the combination of olaparib and palbociclib has synergistic effects against BRCAmut/TNBC both in vitro and in vivo. In olaparib-sensitive MDA-MB-436 cells, the single agent olaparib significantly inhibited cell viability and affected cell growth due to severe DNA damage. In olaparib-resistant HCC1937 and SUM149 cells, single-agent olaparib was ineffective due to potential homologous recombination (HR) repair, and the combination of olaparib and palbociclib greatly inhibited HR during the G2 phase, increased DNA damage and inhibited tumour growth. Inadequate DNA damage caused by olaparib activated the Wnt signalling pathway and upregulated MYC. Further experiments indicated that the overexpression of β-catenin, especially its hyperphosphorylation at the Ser675 site, activated the Wnt signalling pathway and mediated olaparib resistance, which could be strongly inhibited by combined treatment with palbociclib.

Conclusions

Our data provide a rationale for clinical evaluation of the therapeutic synergy of the PARPi olaparib and CDK4/6i palbociclib in BRCAmut/TNBCs with high Wnt signalling activation and high MYC expression that do not respond to PARPi monotherapy.
Rational engineering of xylanase hyper-producing system in Trichoderma reesei for efficient biomass degradation
Authors: Yan Su , Xu Yan , Yu Xiao-Wei
Published: April 2021

Background

Filamentous fungus Trichoderma reesei has been widely used as a workhorse for cellulase and xylanase productions. Xylanase has been reported as the crucial accessory enzyme in the degradation of lignocellulose for higher accessibility of cellulase. In addition, the efficient hydrolysis of xylan needs the co-work of multiple xylanolytic enzymes, which rise an increasing demand for the high yield of xylanase for efficient biomass degradation.

Results

In this study, a xylanase hyper-producing system in T. reesei was established by tailoring two transcription factors, XYR1 and ACE1, and homologous overexpression of the major endo-xylanase XYNII. The expressed xylanase cocktail contained 5256 U/mL xylanase activity and 9.25 U/mL β-xylosidase (pNPXase) activity. Meanwhile, the transcription level of the xylanolytic genes in the strain with XYR1 overexpressed was upregulated, which was well correlated with the amount of XYR1-binding sites. In addition, the higher expression of associated xylanolytic enzymes would result in more efficient xylan hydrolysis. Besides, 2310–3085 U/mL of xylanase activities were achieved using soluble carbon source, which was more efficient and economical than the traditional strategy of xylan induction. Unexpectedly, deletion of ace1 in C30OExyr1 did not give any improvement, which might be the result of the disturbed function of the complex formed between ACE1 and XYR1. The enzymatic hydrolysis of alkali pretreated corn stover using the crude xylanase cocktails as accessory enzymes resulted in a 36.64% increase in saccharification efficiency with the ratio of xylanase activity vs FPase activity at 500, compared to that using cellulase alone.

Conclusions

An efficient and economical xylanase hyper-producing platform was developed in T. reesei RUT-C30. The novel platform with outstanding ability for crude xylanase cocktail production would greatly fit in biomass degradation and give a new perspective of further engineering in T. reesei for industrial purposes.
Under pressure: phenotypic divergence and convergence associated with microhabitat adaptations in Triatominae
Authors: Abad-Franch Fernando , Monteiro Fernando A. , Pavan Márcio G. , Patterson James S. , Bargues M. Dolores , Zuriaga M. Ángeles , Aguilar Marcelo , Beard Charles B. , Mas-Coma Santiago , Miles Michael A.
Published: April 2021

Background

Triatomine bugs, the vectors of Chagas disease, associate with vertebrate hosts in highly diverse ecotopes. It has been proposed that occupation of new microhabitats may trigger selection for distinct phenotypic variants in these blood-sucking bugs. Although understanding phenotypic variation is key to the study of adaptive evolution and central to phenotype-based taxonomy, the drivers of phenotypic change and diversity in triatomines remain poorly understood.

Methods/results

We combined a detailed phenotypic appraisal (including morphology and morphometrics) with mitochondrial cytb and nuclear ITS2 DNA sequence analyses to study Rhodnius ecuadoriensis populations from across the species’ range. We found three major, naked-eye phenotypic variants. Southern-Andean bugs primarily from vertebrate-nest microhabitats (Ecuador/Peru) are typical, light-colored, small bugs with short heads/wings. Northern-Andean bugs from wet-forest palms (Ecuador) are dark, large bugs with long heads/wings. Finally, northern-lowland bugs primarily from dry-forest palms (Ecuador) are light-colored and medium-sized. Wing and (size-free) head shapes are similar across Ecuadorian populations, regardless of habitat or phenotype, but distinct in Peruvian bugs. Bayesian phylogenetic and multispecies-coalescent DNA sequence analyses strongly suggest that Ecuadorian and Peruvian populations are two independently evolving lineages, with little within-lineage phylogeographic structuring or differentiation.

Conclusions

We report sharp naked-eye phenotypic divergence of genetically similar Ecuadorian R. ecuadoriensis (nest-dwelling southern-Andean vs palm-dwelling northern bugs; and palm-dwelling Andean vs lowland), and sharp naked-eye phenotypic similarity of typical, yet genetically distinct, southern-Andean bugs primarily from vertebrate-nest (but not palm) microhabitats. This remarkable phenotypic diversity within a single nominal species likely stems from microhabitat adaptations possibly involving predator-driven selection (yielding substrate-matching camouflage coloration) and a shift from palm-crown to vertebrate-nest microhabitats (yielding smaller bodies and shorter and stouter heads). These findings shed new light on the origins of phenotypic diversity in triatomines, warn against excess reliance on phenotype-based triatomine-bug taxonomy, and confirm the Triatominae as an informative model system for the study of phenotypic change under ecological pressure.

Graphical Abstract
Analysis of an obstetrics point-of-care ultrasound training program for healthcare practitioners in Zanzibar, Tanzania
Authors: Hall Elizabeth A. , Matilsky Danielle , Zang Rachel , Hase Naomasa , Habibu Ali Ali , Henwood Patricia C. , Dean Anthony J.
Published: April 2021

Background

A point-of-care ultrasound education program in obstetrics was developed to train antenatal healthcare practitioners in rural Zanzibar. The study group consisted of 13 practitioners with different training backgrounds: physicians, clinical officers, and nurse/midwives. Trainees received an intensive 2-week antenatal ultrasound course consisting of lectures and hands-on practice followed by 6 months of direct supervision of hands-on scanning and bedside education in their clinical practice environments. Trainees were given a pre-course written exam, a final exam at course completion, and practical exams at 19 and 27 weeks. Trainees were expected to complete written documentation and record ultrasound images of at least 75 proctored ultrasounds. The objective of this study was prospectively to analyze the success of a longitudinal point-of-care ultrasound training program for antepartum obstetrical care providers in Zanzibar.

Results

During the 6-month course, trainees completed 1338 ultrasound exams (average 99 exams per trainee with a range of 42–128 and median of 109). Written exam scores improved from a mean of 33.7% (95% CI 28.6–38.8%) at pre-course assessment to 77.5% (95% CI 71–84%) at course completion (P < 0.0001). Practical exam mean scores improved from 71.2% at course midpoint (95% CI 62.3–80.1%) to 84.7% at course completion (95% Cl 78.5–90.8%) (P < 0.0005). Eight of the 13 trainees completed all training requirements including 75 proctored ultrasound exams.

Conclusion

Trainees improved significantly on all measures after the training program. 62% of the participants completed all requirements. This relatively low completion rate reflects the challenges of establishing ultrasound capacity in this type of setting. Further study is needed to determine trainees’ long-term retention of ultrasound skills and the impact of the program on clinical practice and health outcomes.
Vascular access for renal replacement therapy among 459 critically ill patients: a pragmatic analysis of the randomized AKIKI trial
Authors: Benichou Nicolas , Lebbah Saïd , Hajage David , Martin-Lefèvre Laurent , Pons Bertrand , Boulet Eric , Boyer Alexandre , Chevrel Guillaume , Lerolle Nicolas , Carpentier Dorothée , de Prost Nicolas , Lautrette Alexandre , Bretagnol Anne , Mayaux Julien , Nseir Saad , Megarbane Bruno , Thirion Marina , Forel Jean-Marie , Maizel Julien , Yonis Hodane , Markowicz Philippe , Thiery Guillaume , Schortgen Frederique , Tubach Florence , Ricard Jean-Damien , Dreyfuss Didier , Gaudry Stéphane
Published: April 2021

Background

Vascular access for renal replacement therapy (RRT) is routine question in the intensive care unit. Randomized trials comparing jugular and femoral sites have shown similar rate of nosocomial events and catheter dysfunction. However, recent prospective observational data on RRT catheters use are scarce. We aimed to assess the site of RRT catheter, the reasons for catheter replacement, and the complications according to site in a large population of critically ill patients with acute kidney injury.

Patients and methods

We performed an ancillary study of the AKIKI study, a pragmatic randomized controlled trial, in which patients with severe acute kidney injury (KDIGO 3 classification) with invasive mechanical ventilation, catecholamine infusion or both were randomly assigned to either an early or a delayed RRT initiation strategy. The present study involved all patients who underwent at least one RRT session. Number of RRT catheters, insertion sites, factors potentially associated with the choice of insertion site, duration of catheter use, reason for catheter replacement, and complications were prospectively collected.

Results

Among the 619 patients included in AKIKI, 462 received RRT and 459 were finally included, with 598 RRT catheters. Femoral site was chosen preferentially (n = 319, 53%), followed by jugular (n = 256, 43%) and subclavian (n = 23, 4%). In multivariate analysis, continuous RRT modality was significantly associated with femoral site (OR = 2.33 (95% CI (1.34–4.07),p = 0.003) and higher weight with jugular site [88.9 vs 83.2 kg, OR = 0.99 (95% CI 0.98–1.00),p = 0.03]. Investigator site was also significantly associated with the choice of insertion site (p = 0.03). Cumulative incidence of catheter replacement did not differ between jugular and femoral site [sHR 0.90 (95% CI 0.64—1.25),p = 0.67]. Catheter dysfunction was the main reason for replacement (n = 47), followed by suspected infection (n = 29) which was actually seldom proven (n = 4). No mechanical complication (pneumothorax or hemothorax) occurred.

Conclusion

Femoral site was preferentially used in this prospective study of RRT catheters in 31 French intensive care units. The choice of insertion site depended on investigating center habits, weight, RRT modality. A high incidence of catheter infection suspicion led to undue replacement.
Performance analysis of in-band collision detection for dense wireless networks
Authors: Vermeulen Tom , Reynders Brecht , Rosas Fernando E. , Verhelst Marian , Pollin Sofie
Published: April 2021

With the massive growth of wireless networks comes a bigger impact of collisions and interference, which has a negative effect on throughput and energy efficiency. To deal with this problem, we propose an in-band wireless collision and interference detection scheme based on full-duplex technology. To study its performance, we compare its throughput and energy efficiency with the performance of traditional half-duplex and symmetric in-band full-duplex transmissions. Our analysis considers a realistic protocol and overhead modeling, and a measurement-based self-interference model. Our results indicate that our proposed collision detection scheme can provide significant gains in terms of throughput and energy efficiency in large wireless networks. Moreover, when compared to half-duplex and symmetric full-duplex, our analysis shows that this scheme allows up to 45% more nodes in the network for the same energy consumption per bit. These results suggest that this could be an enabling technology towards efficient, dense wireless networks.
The incubation period during the pandemic of COVID-19: a systematic review and meta-analysis
Authors: Dhouib Wafa , Maatoug Jihen , Ayouni Imen , Zammit Nawel , Ghammem Rim , Fredj Sihem Ben , Ghannem Hassen
Published: April 2021

Background

The aim of our study was to determine through a systematic review and meta-analysis the incubation period of COVID-19. It was conducted based on the preferred reporting items for systematic reviews and meta-analyses (PRISMA). Criteria for eligibility were all published population-based primary literature in PubMed interface and the Science Direct, dealing with incubation period of COVID-19, written in English, since December 2019 to December 2020. We estimated the mean of the incubation period using meta-analysis, taking into account between-study heterogeneity, and the analysis with moderator variables.

Results

This review included 42 studies done predominantly in China. The mean and median incubation period were of maximum 8 days and 12 days respectively. In various parametric models, the 95th percentiles were in the range 10.3–16 days. The highest 99th percentile would be as long as 20.4 days. Out of the 10 included studies in the meta-analysis, 8 were conducted in China, 1 in Singapore, and 1 in Argentina. The pooled mean incubation period was 6.2 (95% CI 5.4, 7.0) days. The heterogeneity (I2 77.1%; p < 0.001) was decreased when we included the study quality and the method of calculation used as moderator variables (I2 0%). The mean incubation period ranged from 5.2 (95% CI 4.4 to 5.9) to 6.65 days (95% CI 6.0 to 7.2).

Conclusions

This work provides additional evidence of incubation period for COVID-19 and showed that it is prudent not to dismiss the possibility of incubation periods up to 14 days at this stage of the epidemic.
Adolescent alcohol consumption: protocol for a scoping review of screening and assessment tools used in Africa
Authors: Briegal Eleanor , Biggane Alice M. , Obasi Angela I.
Published: April 2021

Background

Alcohol consumption is a key public health challenge in sub-Saharan Africa, which has the highest burden of alcohol attributable injury and disease of any region. Excess alcohol use is particularly harmful for adolescents and has been associated with neurocognitive defects and social and emotional problems. Effective screening and assessment tools are necessary to implement, evaluate and monitor interventions to prevent and decrease adolescent alcohol use. Most of these tools have been used among adolescent groups in high income settings; data on their effectiveness in Africa, where much alcohol use is unregulated, is limited. This scoping review will examine and map the range of tools in use and create an evidence base for future research in adolescent alcohol prevention and control in Africa.

Methods

The review will include all relevant study designs and grey literature. Inclusion and exclusion criteria have been designed using the Population – Concept – Context framework, and two reviewers will independently screen titles, abstracts and then full text to determine eligibility of articles. The Cochrane Library, MEDLINE, CINAHL and Global Health data bases will be searched for peer reviewed publications. The search strategy for grey literature will include Google searches and searches in websites of pertinent professional bodies and charities. The methodological framework proposed by Arksey and O’Malley and adaptations by the Joanna Briggs Institute and Levac et al. will be used. An iterative approach to charting, collating, summarising and reporting the data will be taken, with the development of charting forms and the final presentation of results led by the extracted data.

Results

This scoping review protocol describes a secondary analysis of data already collected to explore and map alcohol consumption measurement tools in adolescents in Africa.

Conclusions

It is anticipated that our findings will provide an evidence base surrounding tools used to measure adolescent alcohol consumption in Africa. These findings are likely to be useful in informing future research, policy and public health strategies. Findings will be disseminated widely through peer-reviewed publication and in various media, for example, conferences, congresses or symposia.

Systematic review registration

Scoping Review Registration: Open Science Framework (https://osf.io/bjhgw/)
Risk factors for nosocomial rectal colonization with carbapenem-resistant Acinetobacter baumannii in hospital: a matched case–control study
Authors: Meschiari Marianna , Kaleci Shaniko , Orlando Gabriella , Selmi Silvia , Santoro Antonella , Bacca Erica , Menozzi Marianna , Franceschini Erica , Puzzolante Cinzia , Bedini Andrea , Sarti Mario , Venturelli Claudia , Vecchi Elena , Mussini Cristina
Published: April 2021

Background

During the last decade carbapenem-resistantAcinetobacter baumannii(CRAB) became hyper-endemic in hospitals due to difficult to control spreading. Our aim is to identify risk factors for nosocomial rectal CRAB colonization in an endemic hospital.

Methods

A retrospective matched case–control study (ratio 1:2) with a prospective inclusion of cases and concurrent selection of controls was conducted from January 2017 to December 2018 in a tertiary-care hospital. Universal active surveillance for CRAB was implemented. Univariate and multivariate logistic regression was carried out using a stepwise selection method to compare prognostic factors between cases and controls. A sub-analysis was carried out according to the type of department.

Results

Forty-five cases with nosocomial rectal CRAB colonization and 90 controls were included. One hundred and two (75%) patients were hospitalized in medical departments. At multivariable analysis significant risk factors associated with CRAB colonization were: use of permanent devices (OR 10.15, 95% CI 2.27–45.39;P = 0.002), mechanical ventilation (OR 40.01, 95% CI 4.05–395.1;P = 0.002), urinary catheters (OR 4.9, 95% CI 1.52–16.19;P = 0.008), McCabe score (OR 5.45, 95% CI 1.87–15.89;P = 0.002), length of stay (OR 1.03, 95% CI 1.01–1.05;P = 0.002), carbapenem use (OR 5.39, 95% CI 1.14–25.44;P = 0.033). The sub-analysis showed that patients admitted to different departments had different risk factors. In geriatric department a fatal disease and a longer hospital stay represented significant risk factors both in univariate and multivariate analysis, while in internal medicine department the use of permanent devices, current antibiotic therapy and antibiotic polytherapy represented significant risk factors for CRAB at the univariate analysis, also confirmed in multivariate analysis.

Conclusions

Our data suggest that active surveillance for rectal CRAB colonization should be addressed to patients with an unfavourable prognosis, longer hospitalizations and carriers of multiple devices. To counter CRAB spreading in endemic settings, clinicians must limit the use of carbapenems, and reinforce interventions aimed at proper use of devices.
A moderated mediation analysis of the relationship between a high-stakes English test and test takers’ extracurricular English learning activities
Authors: Zhang Jing
Published: April 2021

This study investigated the relationship between a large-scale and high-stakes English test and test takers’ learning behavior. Specifically, it explored whether and how the National Matriculation English Test (NMET) influenced test takers’ extracurricular English learning activities under the Chinese Mainland educational context. Based on Bandura’s triadic reciprocal determinism theory, this study proposed a distal mediation model and employed covariance-based Structural Equation Modeling to test the model. The data were collected via a cross-sectional survey with 470 test takers. The results showed that test takers’ perceptions of the examination exerted direct and indirect effects on their extracurricular English learning activities, and that test takers’ perceived self-efficacy for self-regulated learning and academic achievement were two important factors mediating the relationship between their perceptions of the test and extracurricular learning. Furthermore, test takers’ perceptions of the exam-approaching have diverse moderating effects on different mediation effects. This study suggests that introducing the triadic reciprocal determinism theory helps understand how an examination influences learning. It also highlights the role of test takers’ perceptions of an examination and their perceived self-efficacy in predicting a test’s impact on learning.
Investigation into the predictive performance of colorimetric sensor strips using RGB, CMYK, HSV, and CIELAB coupled with various data preprocessing methods: a case study on an analysis of water quality parameters
Authors: Phuangsaijai Nutthatida , Jakmunee Jaroon , Kittiwachana Sila
Published: April 2021

The potential use of colorimetric sensors has received significant attention due to its feasibility for use in various applications. After reacting with a sample, the image of the colorimetric sensor can be captured and converted into digital data using several different color models. The analytical data can then be processed with various chemometric methods. This research study investigated the predictive performance of calibration models established using color models commonly used in analytical chemistry including RGB, CMYK, HSV and CIELAB. A total of eight commercially available colorimetric sensors were used to determine the presence of manganese (Mn2+), copper (Cu2+), iron (Fe2+/Fe3+), nitrate (NO3–), phosphate (PO43–), sulfate (SO42–), as well as total hardness and pH values. As external validation tests, real water samples collected in Chiang Mai, Thailand were used. Based on the resulting data obtained using the synthetic test samples, the color that was most similar to the appearing color of the chemical sensor could offer satisfactory results. However, it was not always the case especially when the strips composed of multiple colorimetric sensors or sensor array were used. When tested with external validation, the predictive performance could be improved using appropriate data preprocessing and, in this research study, a normalization method was recommended to guarantee the accuracy of the calibration models.
Identifying key processes and sectors in the interaction between climate and socio-economic systems: a review toward integrating Earth–human systems
Authors: Tachiiri Kaoru , Su Xuanming , Matsumoto Ken’ichi
Published: April 2021

For the purpose of identifying the key processes and sectors involved in the interaction between Earth and socio-economic systems, we review existing studies on those processes/sectors through which the climate impacts socio-economic systems, which then in turn affect the climate. For each process/sector, we review the direct physical and ecological impacts and, if available, the impact on the economy and greenhouse gas (GHG) emissions. Based on this review, land sector is identified as the process with the most significant impact on GHG emissions, while labor productivity has the largest impact on the gross domestic product (GDP). On the other hand, the energy sector, due to the increase in the demand for cooling, will have increased GHG emissions. Water resources, sea level rise, natural disasters, ecosystem services, and diseases also show the potential to have a significant influence on GHG emissions and GDP, although for most of these, a large effect was reported only by a limited number of studies. As a result, more studies are required to verify their influence in terms of feedbacks to the climate. In addition, although the economic damage arising from migration and conflict is uncertain, they should be treated as potentially damaging processes.
Tree growth is more limited by drought in rear-edge forests most of the times
Authors: Camarero J. Julio , Gazol Antonio , Sangüesa-Barreda Gabriel , Vergarechea Marta , Alfaro-Sánchez Raquel , Cattaneo Nicolás , Vicente-Serrano Sergio M.
Published: April 2021

Background

Equatorward, rear-edge tree populations are natural monitors to estimate species vulnerability to climate change. According to biogeographical theory, exposition to drought events increases with increasing aridity towards the equator and the growth of southern tree populations will be more vulnerable to drought than in central populations. However, the ecological and biogeographical margins can mismatch due to the impact of ecological factors (topography, soils) or tree-species acclimation that can blur large-scale geographical imprints in trees responses to drought making northern populations more drought limited.

Methods

We tested these ideas in six tree species, three angiosperms (Fagus sylvatica, Quercus robur, Quercus petraea) and three gymnosperms (Abies alba, Pinus sylvestris and Pinus uncinata) by comparing rear-edge tree populations subjected to different degrees of aridity. We used dendrochronology to compare the radial-growth patterns of these species in northern, intermediate, and southern tree populations at the continental rear edge.

Results and conclusions

We found marked variations in growth variability between species with coherent patterns of stronger drought signals in the tree-ring series of the southern populations of F. sylvatica, P. sylvestris, and A. alba. This was also observed in species from cool-wet sites (P. uncinata and Q. robur), despite their limited responsiveness to drought. However, in the case of Q. petraea the intermediate population showed the strongest relationship to drought. For drought-sensitive species as F. sylvatica and P. sylvestris, southern populations presented more variable growth which was enhanced by cool-wet conditions from late spring to summer. We found a trend of enhanced vulnerability to drought in these two species. The response of tree growth to drought has a marked biogeographical component characterized by increased drought sensitivity in southern populations even within the species distribution rear edge. Nevertheless, the relationship between tree growth and drought varied between species suggesting that biogeographical and ecological limits do not always overlap as in the case of Q. petraea. In widespread species showing enhanced vulnerability to drought, as F. sylvatica and P. sylvestris, increased vulnerability to climate warming in their rear edges is forecasted. Therefore, we encourage the monitoring and conservation of such marginal tree populations.
Psychometric properties of FACIT-Fatigue in systemic lupus erythematosus: a pooled analysis of three phase 3 randomised, double-blind, parallel-group controlled studies (BLISS-SC, BLISS-52, BLISS-76)
Authors: Rendas-Baum Regina , Baranwal Nishtha , Joshi Ashish V. , Park Josephine , Kosinski Mark
Published: April 2021

Background

Fatigue is a key symptom in patients with systemic lupus erythematosus (SLE), and regulatory bodies recommend its assessment in clinical trials of SLE therapies.

Methods

This post hoc pooled analysis of the three BeLimumab In Subjects with Systemic lupus erythematosus (BLISS) Phase 3 randomised, double-blind, parallel-group controlled trials evaluated the measurement properties of the Functional Assessment of Chronic Illness Therapy (FACIT)-Fatigue. Patients (N = 2520) completed the FACIT-Fatigue every 4 weeks from baseline until the end of each study period. Internal consistency, test–retest reliability, convergent validity, and ability to detect changes in SLE were evaluated for the FACIT-Fatigue.

Results

The FACIT-Fatigue showed good internal consistency reliability (Cronbach’s alpha > 0.90), very good test–retest reliability (0.76 ≤ intraclass correlation coefficient ≤ 0.92), and moderate-strong convergent validity (0.49 ≤ |r| ≤ 0.86) against scale and summary measure scores from the Short Form 36 Health Survey Version 2. Correlations between FACIT-Fatigue and British Isles Lupus Assessment Group (BILAG) General/Musculoskeletal scores (0.24 ≤ |r| ≤ 0.43) supported convergent validity. Correlations between FACIT-Fatigue and the Safety of Estrogens in Lupus Erythematosus National Assessment-Systemic Lupus Erythematosus Disease Activity Index (SELENA-SLEDAI) scores and SLE annualised flare rate were weak but in the expected direction (ranging from − 0.02 to − 0.25). Known-groups validity testing showed that the FACIT-Fatigue can significantly discriminate between patient groups with differing scores for SELENA-SLEDAI, BILAG (General and Musculoskeletal) ratings, and Physician’s Global Assessment (PGA). Patients showing improvement in PGA and meeting the BILAG responder criteria had significantly higher mean improvement in FACIT-Fatigue scores than those without improvements in either measure (Week 52 mean score difference [95% confidence interval]: − 4.0 [− 5.0, − 3.0] and −2.2 [−3.1, −1.2], respectively; bothp < 0.0001). The range of important (i.e. meaningful) change in FACIT-Fatigue, based on multiple anchors, was 3–6 points.

Conclusions

The FACIT-Fatigue demonstrated adequate psychometric properties in patients with SLE. The body of evidence from the three BLISS trials (both pooled and individually) supports the FACIT-Fatigue as a reliable and valid measure of SLE-related fatigue in clinical trials.

Clinical trial identifiers

BLISS-SC (NCT01484496), BLISS-52 (NCT00424476), and BLISS-76 (NCT00410384).
The effect of gender, age, and body mass index on the medial and lateral posterior tibial slopes: a magnetic resonance imaging study
Authors: Aljuhani Wazzan S. , Qasim Salman S. , Alrasheed Abdullah , Altwalah Jumanah , Alsalman Mohammed J.
Published: April 2021

Background

The posterior tibial slope (PTS) is crucial in knee joint stability and in maintaining the natural movement of the knee. An increase in the PTS is associated with various knee pathologic conditions, such as anterior cruciate ligament (ACL) injury and anterior tibial translation (ATT). In the present study, we aimed to establish native medial and lateral PTS values for adult Saudis and to identify any association between PTS and gender, age, and body mass index (BMI).

Materials and methods

A total of 285 consecutive, normal, magnetic resonance imaging (MRI) studies of the knee were included in the study. The PTS was measured using the proximal anatomical axis of the tibia. The Kruskal-Wallis test was used to compare the medial and lateral PTS angles between age groups. The difference between the medial and lateral posterior tibial slopes was assessed using the Wilcoxon signed-rank test. The Mann-Whitney U test was performed to compare the medial and lateral PTS angles between men and women. Age, gender, and BMI were analyzed by multivariate linear regression to determine whether they positively predict the medial and lateral PTS angles.

Results

The mean physiological medial PTS was 5.86 ± 3.0° and 6.61 ± 3.32°, and the lateral PTS was 4.41 ± 3.35° and 4.63 ± 2.85° in men and women, respectively. This difference showed no statistically significant gender dimorphism (p > 0.05). The medial PTS was significantly larger than the lateral PTS (p < 0.0001). There was no statistically significant difference in the medial and lateral PTS angles between age groups (p > 0.05). Higher BMI was significantly associated with a steeper medial PTS (p = 0.001).

Conclusions

This study provided native values for medial and lateral PTS angles in Saudis, which can assist surgeons in maintaining normal knee PTS during surgery. The PTS was not influenced by age. The medial PTS was significantly larger than the lateral PTS in men and women. The PTS showed no significant gender dimorphism. BMI was significantly associated with the medial PTS.
Long-term loss assessment of coastal bridges from hurricanes incorporating overturning failure mode
Authors: Zhu Deming , Li Yaohan , Dong You , Yuan Peng
Published: April 2021

Coastal highway bridge is an essential component of the transportation system but threatened by natural hazards such as hurricanes. Damaged highway bridges result in not only transportation disruption, but also tremendous financial, societal, and life loss. Therefore, vulnerability and loss assessments of bridges under hurricane events are becoming primary concerns for decision-makers. This study provides an elaborate framework to assess the vulnerability and long-term loss of coastal bridges subjected to hurricane hazards based on three-dimensional (3D) numerical analyses. A 3D Computational Fluid Dynamics (CFD) numerical model is established to investigate wave-bridge interaction and a Finite Element (FE) model is established for the bridge to calculate structural responses under wave impacts. Based on the numerical results, the effects of wave force and overturning moment on structural capacity are studied and a probabilistic vulnerability model is developed. Structural demand, capacity, and limit states are determined, respectively. Uncertainties associated with wave parameters, structural capacity, and material properties, and the resulting consequences are considered. Then, fragility curves are calculated, and long-term damage loss is assessed. The proposed approach can benefit the management and design of coastal bridges against the impacts of hurricane hazards.
Is Independent Clinical Research Possible in Low- and Middle-Income Countries? A Roadmap to Address Persistent and New Barriers and Challenges
Authors: Barrios Carlos H. , Mano Max S.
Published: April 2021

Cancer is an increasing and significant problem for both high- and low- and middle-income countries. Basic, translational, and clinical research efforts have been instrumental in generating the outstanding improvements we have witnessed over the last few decades, answering important questions, and improving patient outcomes. Arguably, a substantial portion of currently ongoing research is sponsored by the pharmaceutical industy and specifically addresses questions under industry interests, most of which apply to high-income countries, leaving behind problems related to the much larger and underserved population of patients with cancer in low- and middle-income countries. In this scenario, discussing independent academic research is an important challenge, particularly for these countries. Although different countries and institutions face different problems while establishing independent research agendas, some generalizable barriers can be identified. A solid regulatory and ethical framework, a strong and sustainable technical supporting infrastructure, and motivated and experienced investigators are all paramount to build a viable and productive academic research program. Securing funding for research, although not the only hurdle, is certainly one of the most basic hurdles to overcome. Noticeably, and as an added impediment, public and governmental support for cancer research has been decreasing in high-income countries and is almost nonexistent in the rest of the world. We propose an initial careful diagnostic assessment of the research resource scenario of each institution/country and adjustment of the strategic development plan according to four different research resource restriction levels. Although not necessarily applicable to all situations, this model can be helpful if adjusted to each local or regional situation.
Migration and Uncertainties: The Ordeal of Syrian Women Refugees with Missing Spouses
Authors: Gülerce Hakan
Published: April 2021

The ongoing civil war in Syria has caused millions of people to migrate across the border in order to save their lives. However, as they reached on safer grounds, they were left homeless. This migration has been occurring intensively to countries with borders neighboring Syria. Turkey has the highest number of refugees among Syria’s neighboring countries, harboring close to four million Syrian refugees. There are only a small proportion of the Syrian refugee population living in temporary accommodation centers as the larger part is scattered throughout the various provinces of Turkey. Although they encounter certain problems that relate to different provinces, most of the troubles experienced by the refugees are common to all of them.
Psychosis in Huntington´s Disease. a rare and Under-Investigated Psychiatric Manifestation
Authors: Gonçalves Maria João , Ferreira Carla , Saraiva Rodrigo , Sereijo Carolina , Castanheira Lígia , Fernandes Elsa , Amaro Rita , Mendes Tiago , Guedes Leonor Correia , Abreu Manuela , Chendo Inês
Published: April 2021

Introduction: Psychotic symptoms are among the least prevalent and under-investigated psychiatric manifestations (PM) of Huntington's disease (HD). Case report: We herein report a case of a 31-year-old male patient who presented PM with a predominance of negative symptoms, without any significant abnormal movement. HD was diagnosed based on positive DNA analysis and family history. HD imposes longitudinal follow-up through a multidisciplinary approach in order to improve the quality of life and prognosis. Conclusion: This case report highlights the importance of comprehending the PM in the initial presentation of HD so that the diagnosis is not delayed until the onset of motor symptoms.
SAPHIR: a Shiny application to analyze tissue section images
Authors: Germani Elodie , Lelouard Hugues , Fallet Mathieu
Published: April 2021

Study of cell populations in tissues using immunofluorescence is a powerful method for both basic and medical research. Image acquisitions performed by confocal microscopy notably allow excellent lateral resolution and more than 10 parameter measurements when using spectral or multiplex imaging. Analysis of such complex images can be very challenging and easily lead to bias and misinterpretation. Here, we have developed the Shiny Analytical Plot of Histological Image Results (SAPHIR), an R shiny application for histo-cytometry using scatterplot representation of data extracted by segmentation. It offers many features, such as filtering of spurious data points, selection of cell subsets on scatterplot, visualization of scatterplot selections back into the image, statistics of selected data and data annotation. Our application allows to characterize labeled cells, from their phenotype to their number and location in the tissue, as well as their interaction with other cells. SAPHIR is available from: https://github.com/elodiegermani/SAPHIR
Public perception of NHS general practice during the first six months of the COVID-19 pandemic in England
Authors: Duncan Lorna J. , Cheng Kelly F.D.
Published: April 2021

Background: In March 2020, the delivery of NHS general practice consultations was rapidly modified to mitigate against the spread of COVID-19. Remote triage and consultations became the default, with adapted models for face-to-face contact if clinically required. This study aimed to gain insight into public perception of these adaptations.

Methods: Two online surveys were developed, and conducted between August and September 2020. Survey A, open to anyone receiving the link to it, considered respondents’ experiences of healthcare contacts since March 2020, and their understanding of the adapted delivery. Survey B, open to survey A respondents only, then considered how healthcare communication had been received and individual preferences for this. Survey participation was voluntary.

Results: The views and experiences of 150 members of the public were obtained. 105 had considered contacting general practice, although half avoided this or delayed doing so for longer than usual. While some patients did so ‘to help the NHS’, others experienced reduced access for a variety of reasons including COVID-19 safety concerns. Some however reported benefitting from remote consultation availability and regular texts/emails from their practice.

68% (102/150) of respondents were unaware that patients with COVID-19 were seen separately from other patients during general practice appointments. 27% of those in survey B who had avoided or delayed contact said they would have felt more comfortable contacting general practice had they known about this.

Conclusions: Experience and use of the adapted general practice models varied. Some patients felt their access to healthcare was reduced, often due to technological requirements. For some who found attending face-to-face appointments difficult however, remote contact was advantageous. Most patients surveyed were unaware of the COVID-19 control measures in place during face-to-face general practice consultations. Assessment of adapted delivery model accessibility and clearer public messaging about the changes may help reduce inequalities.
Understanding how human rights law affects nursing practice
Authors: Griffith Richard
Published: April 2021

In the first of a series of articles Richard Griffith, Senior Lecturer in Health Law at Swansea University, discusses the fundamental nature of human rights and their importance to nursing
Reducing dog bites and promoting safety: Merseyside Dog Safety Partnership
Authors: Schofield Rebecca , Oxley James A. , Westgarth Carri
Published: April 2021

The UK is well known for being a nation of dog lovers and recent evidence has shown the positive impact owning a dog can have on human health and wellbeing. However, for people who have experienced a dog bite injury, this can be quite a different story.
Small animal Review
Authors: Craig Mark
Published: April 2021

In this month's Small Animal Review three published papers relating to animal gut health from other veterinary journals are summarised. The papers for this issue focus on the canine microbiome in gastrointestinal disease and the microbiota-related changes that occur as a result of canine diabetes mellitus, as well as a literature review on the impact of exposure to dietary emulsifiers on bowel and metabolic health.
Autologous meniscus fragments embedded in atelocollagen gel enhance meniscus repair in a rabbit model
Authors: Matsubara Norimasa , Nakasa Tomoyuki , Ishikawa Masakazu , Tamura Takayuki , Adachi Nobuo
Published: April 2021

Aims

Meniscal injuries are common and often induce knee pain requiring surgical intervention. To develop effective strategies for meniscus regeneration, we hypothesized that a minced meniscus embedded in an atelocollagen gel, a firm gel-like material, may enhance meniscus regeneration through cell migration and proliferation in the gel. Hence, the objective of this study was to investigate cell migration and proliferation in atelocollagen gels seeded with autologous meniscus fragments in vitro and examine the therapeutic potential of this combination in an in vivo rabbit model of massive meniscus defect.

Methods

A total of 34 Japanese white rabbits (divided into defect and atelocollagen groups) were used to produce the massive meniscus defect model through a medial patellar approach. Cell migration and proliferation were evaluated using immunohistochemistry. Furthermore, histological evaluation of the sections was performed, and a modified Pauli’s scoring system was used for the quantitative evaluation of the regenerated meniscus.

Results

In vitro immunohistochemistry revealed that the meniscus cells migrated from the minced meniscus and proliferated in the gel. Furthermore, histological analysis suggested that the minced meniscus embedded in the atelocollagen gel produced tissue resembling the native meniscus in vivo. The minced meniscus group also had a higher Pauli’s score compared to the defect and atelocollagen groups.

Conclusion

Our data show that cells in minced meniscus can proliferate, and that implantation of the minced meniscus within atelocollagen induces meniscus regeneration, thus suggesting a novel therapeutic alternative for meniscus tears. Cite this article: Bone Joint Res 2021;10(4):269–276.