bluuebunny/crossref_metadata_2025 huggingface dataset:
Mengembangkan Sportivitas Melalui Permainan Modifikasi Hot (Hoki Tiup)
Authors: Sufiati Vivi , Damayanti Dwinda
Published: January 2022

This study aims to determine the development of sportsmanship in children through a modified game of HOT (Hoki Tiup) during the pandemic. The research was conducted at Rumah Belajar All Kids. The research subjects were selected by purposive sampling, namely 4 children aged 5-6 years who did limited face-to-face learning. This research is a qualitative research with a case study approach. Methods of data collection used interviews, observation, and document study. The data analysis was used the data reduction stage, data presentation and conclusion drawing. The results of this study indicate that sportsmanship develops from the aspects of respect, fairness, and acceptance of the results of the game. Respect is shown through natural expressions when experiencing victory or defeat. A fair attitude is marked by the child starting to obey the rules of the game when playing with all his friends.The child receives the final result of the game.
Penanaman Karakter Peduli Lingkungan Melalui Pengajaran Fikih Ekologi Pada Anak Usia Dini
Authors: Fathurahman M
Published: January 2022

This research is a library research, with the title Cultivating Environmentally Concerned Characters through Teaching Ecological Jurisprudence in Early Childhood. This research uses library materials as the main data source, and the main references in this research are ecological and Islamic books, character education and books on early childhood. The purpose of this study is combining two materials at once, namely environmental fiqh material and its role in shaping the character of early childhood. The results showed. First, environmental damage or the current ecological crisis is not actually due to ecological succession factors. However, the damage occurred precisely because of an anthropogenic disaster by human activities. Second, in environmental fiqh it contains material that is very important to study, namely maqoshidu shari'ah, especially hifdzu al-bi'ah. Thirdly, environmental care character is very urgent, especially given to early childhood, and for now studying this section is an obligation.
Pseudomonas Tumuqiensis Sp. Nov., Isolated From Greenhouse Soil
Authors: Kong Delong , Li Qingqing , Zhou Yanzheng , Wang Yan , Jiang Xu , Wang Zhiye , Ruan Zhiyong
Published: January 2022

A Gram-stain-negative, aerobic, rod-shaped and motile bacterium, named LAMW06T, was isolated from greenhouse soil in Beijing, China. In the 16S rRNA gene sequence comparison, strain LAMW06T had the highest similarity with Pseudomonas cuatrocienegasensis 1NT was 97.4%. Phylogenetic analysis base on the 16S rRNA and three housekeeping gene sequences (gyrB, rpoB and rpoD) indicated that LAMW06T represented a member of the genus Pseudomonas. The genome sequence size of the isolate was 5.5 Mb, with the DNA G+C content of 63.5 mol%. The average nucleotide identity and DNA–DNA hybridization values between strain LAMW06T and closely related members of Pseudomonas borbori R-20821T, Pseudomonas taeanensis MS-3T and Pseudomonas cuatrocienegasensis 1NT were 90.9%, 82.4%, 81.5 % and 43.0%, 25.9%, 24.6 % respectively. The major fatty acids contained summed feature 3 (C16:1 ω6c and/or C16:1 ω7c), C18:1 ω7c and C16:0. The primary respiratory quinone was ubiquinone-9. The main polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, six aminophospholipids, six phospholipids, one aminolipid and one glycolipid. According to the genotypic, phylogenetic and chemotaxonomic data, strain LAMW06T represents a novel species within the genus Pseudomonas, for which the name Pseudomonas tumuqiensis sp. nov. is proposed. The type strain is LAMW06T (=GDMCC 1.2003T =KCTC 72829T).
An Analysis of the Impact of Clean and Non-clean Energy Consumption on Economic Growth and Carbon Emission: Evidence from PIMC Countries
Authors: Ali Arshad , Radulescu Magdalena , Lorente Daniel Balsalobre , Hoang Viet-Ngu (Vincent)
Published: January 2022

This study empirically estimates the impact of clean and non-clean energy consumption on economic growth and carbon dioxide emissions within the framework of the environmental Kuznets curve and pollution haven hypothesis in the case of PIMC countries from 1980 to 2019. The results of the panel cointegration test proposed by Westerlund (2007) show a long-term equilibrium relationship among the variables of each designated model. The long-term elasticities of economic growth and carbon emission estimated by AMG, CCEMG and MG estimators indicate that both clean and non-clean energy consumption have a significant impact on economic growth, while carbon emission hinders growth. The results also reveal that economic growth, non-clean energy consumption and interaction between trade openness and non-clean energy consumption have a driving effect on carbon dioxide emission, however, clean energy consumption is found to reduce carbon emission. In addition, the analysis confirms the existence of the inverted U-shaped environmental Kuznets curve and pollution haven hypothesis in the panel of PIMC economies. Finally, there is a one-way causality from non-clean energy consumption to economic growth, but no such causation exists between clean energy consumption and economic growth. The objective of sustained economic growth with a safe environment may be achieved by encouraging clean energy consumption in the PIMC economies.
“i Did Whatever They Wanted Me to Do”: a Qualitative Secondary Analysis Using Reproductive Justice to Explore Sexual Violence Among Justice-involved Latina Mothers
Authors: Crawford Allison D. , McGlothen-Bell Kelly , Cleveland Lisa
Published: January 2022

Background: One in three women experience sexual violence during their lifetime; however, little is known about this phenomenon with respect to justice-involved Latina mothers. Using the reproductive justice framework as a theoretical lens, we examined sexual violence in Latina mothers who had experienced incarceration and were thus involved in the justice system.Methods: This was a secondary analysis of a qualitative data set. The reproductive justice framework provided a theoretical lens for examining the women’s rights to bodily autonomy, to have or not have children, and to live in safe, sustainable environments given the intersection of incarceration and sexual violence.Results: Women (N = 12) recounted their experiences of sexual violence after having been incarcerated. Incarceration and resulting sexual violence led to discrimination, limited bodily autonomy, sexual exploitation, substance use, depression, anxiety, re-traumatization, recidivism, underreporting of violence, underutilization of healthcare resources, strained relationships, family separation, and unsafe environments. Conclusions: More research is needed to understand the social, economic, and political contexts that perpetuate sexual violence among justice-involved women. Universal healthcare, participatory research, changing cultural mindsets, decriminalization of sex work, and more comprehensive tracking and prosecution of sexual predators may be key to ending sexual violence in justice-involved mothers.
Structures of LIG1 engaging with mutagenic mismatches inserted by polβ
in base excision repair
Authors: Caglayan Melike , Tang Qun , McKenna Robert
Published: January 2022

DNA ligase I (LIG1) catalyzes final ligation step following DNA polymerase (pol) β gap filling and an incorrect nucleotide insertion by polβ creates a nick repair intermediate with mismatched end at the downstream steps of base excision repair (BER) pathway. Yet, how LIG1 discriminates against the mutagenic 3'-mismatches at atomic resolution remains undefined. Here, we determined X-ray structures of LIG1/nick DNA complexes with G:T and A:C mismatches and uncovered the ligase strategies that favor or deter ligation of base substitution errors. Our structures revealed that LIG1 active site can accommodate G:T mismatch in a similar conformation with A:T base pairing, while it stays in the LIG1-adenylate intermediate during initial step of ligation reaction in the presence of A:C mismatch at 3'-strand. Moreover, we showed mutagenic ligation and aberrant nick sealing of the nick DNA substrates with 3'-preinserted dG:T and dA:C mismatches, respectively. Finally, we demonstrated that AP-Endonuclease 1 (APE1), as a compensatory proofreading enzyme, interacts and coordinates with LIG1 during mismatch removal and DNA ligation. Our overall findings and ligase/nick DNA structures provide the features of accurate versus mutagenic outcomes at the final BER steps where a multi-protein complex including polβ, LIG1, and APE1 can maintain accurate repair.
Fear of Cancer Recurrence Is Highly Prevalent and Linearly Associates With Anxiety and Depression in Chinese Breast Cancer Patients
Authors: Li Zhensheng , Li Yue , Liu Yunjiang , Zhang Jun , Ji Xiaohui , Zhao Zhiping , Duan Xuejuan , Geng Wenhui , Yin Junpu
Published: January 2022

Objective: To characterize the fear of cancer recurrence (FCR) and its relationship with anxiety and depression and quality of life (QoL) among Chinese breast cancer (BC) patients in China. Methods: Patients completed the questionnaires of QLQ-C30, QLQ-BR32 and HAD to assess FCR, QoL, anxiety and depression before radiotherapy. A cross-sectional analysis was performed. Chi-square and non-parametric tests and multivariate ordinal logistic regressions (mOLR) were utilized for reference analysis. Final covariates included age, BMI, TNM, surgery, chemotherapy, pain, and sleep disturbance. Results: From July 2015 to December 2016, 463 patients were prospectively enrolled. Their age mean (range) were 47 (19 - 89) years old. In total, 327 patients (70.6%) reported having FCR ‘a little bit’ (51.2%), ‘some’ (12.1%) and ‘very much’ (7.3%) in the past week. FCR severity ordered above (incl. ‘no’) was associated with anxiety score (median 1.5, 5.0, 7.0, 8.5 and level (‘abnormal’ rate 0%, 3.4%, 12.5%, 26.5%), depression score (median 2.0, 4.0, 6.0, 6.5) and level (‘abnormal’ rate 2.2%, 3.4%, 5.4%, 17.7%) (all p<0.001). mOLR showed that compared to ‘no’, three higher levels of FCR were associated with one level increase of anxiety with OR (p) as 1.983 (0.076), 4.291 (0.001), 8.282 (<0.001) and depression with OR (p) as 1.903 (0.062), 2.262 (0.065), 4.205 (0.004), respectively. FCR severity also was inversely associated with most QoL function scores (p<0.001). Conclusions: FCR was prevalent in Chinese BC patients and linearly associated with anxiety, depression and low QoL. It seems that a single-item question for FCR is a valid surrogate tool for distress screening in this population.
Potential HNF1A-AS1/miR-214/INHBA signal regulation mechanism in colorectal cancer based on bioinformatics analysis and validation
Authors: Zhang Xuan , Wu Tao , Ding Rong , Qin Rujia , Zhou Yongchun , Wang Xiaoxiong , Shang ZhongJun , Li GuoYu , Dong Chao , Yang Renfang , Li Jinsha , Li Yunfeng
Published: January 2022

Preceding studies have identified that noncoding RNA plays a significant role in the occurrence and development of tumors. Colorectal cancer (CRC) has attracted increasing attention due to its high incidence and mortality rate. Based on Cancer Genome Atlas (TCGA) database analysis, it was found that compared with normal tissues, HNF1A-AS1 and INHBA were highly expressed in CRC tissues; miR-214 was relatively low expressed, and it is predicted to specifically target the3' untranslated region (3'UTR region) of INHBA. Besides, the result was consistent with the quantitative reverse transcription PCR (RT-qPCR) verification results of 17 CRC cases and adjacent tissues collected clinically. Western Blot (WB) manifested that INHBA protein was highly expressed in CRC tissues, which was consistent with the results of CRC cell lines (HT29, SW480). Immunohistochemical (IHC) staining demonstrated that INHBA protein was brownish yellow, overwhelming majority of INHBA protein were located in the cytoplasm, and expression level was significantly higher than that in adjacent tissues. Based on previous studies, the biological process of INHBA-mediated TGF-β/Smad signaling pathway inducing the proliferation and invasion of CRC has been partially confirmed, but the upstream signaling molecules and mechanisms which regulating INHBA remain unclear. Herein, benefiting from bioinformatics, preliminary experimental results and previous research, they provide basis for the follow-up study on the regulation of HNF1A-AS1/miR-214/INHBA signal axis in CRC.
Effect of different basin materials on the performance of hemispherical distiller with mirrors and energy storage material
Authors: Attia Mohammed El Hadi , Essa Fadl Abdelmonem , Abdelgaied Mohamed , Kabeel Abd elnaby
Published: January 2022

Numerous studies are being conducted on solar desalination systems with creating new designs to improve the efficiency and yield of these systems. Hemispherical solar still has a relatively large throughput because of its large exposure and evaporation surface areas compared to the other distiller designs. To get the optimal conditions of the parameters that provide the highest productivity of the hemispherical still, three hemispherical basin stills were fabricated and tested. The experiments were performed in three stages: In the first one, the traditional hemispherical solar still THSS (a reference distiller) was compared by THSS with internal reflective mirrors (THSS-IRM). In the second stage, the effect of using different basin metals (THSS with basin metal of zinc (THSS-IRMZ) and basin metal of copper (THSS-IRMC)) with internal reflective mirrors was studied. In the third stage, the two distillers were investigated under using energy storage medium (30 g/L sand grains for each). The THSS with internal reflective mirrors, basin metal of zinc, and energy storage medium is abbreviated by THSS-IRMZSG. Also, THSS with internal reflective mirrors, basin metal of copper, and energy storage medium is abbreviated by THSS-IRMCSG. Moreover, THSS with internal reflective mirrors and energy storage medium is abbreviated by THSS-IRMSG. The results showed that the combination of using internal reflective mirrors, basin material (copper) and energy storage medium provided the best improvement of hemispherical distillation device. The maximum cumulative yield of THSS-IRMCSG was 11.9 L/m².day, while the reference distillation device gave a total yield of 4.65 L/m².day. So, the productivity was improved by around 156%.
Nationwide Survey on Family-perceived Experiences of Patients With Cancer of Unknown Primary Site
Authors: Ishida Kyoko , SATO Kazuki , KOMATSU Hirokazu , MORITA Tatsuya , AKECHI Tatsuo , UCHIDA Megumi , MASUKAWA Kento , IGARASHI Naoko , KIZAWA Yoshiyuki , TSUNETO Satoru , SHIMA Yasuo , MIYASHITA Mitsunori , ANDO Shoko
Published: January 2022

Purpose: Cancer of unknown primary site (CUP) is an aggressive disease with poor prognosis. As research on the experiences of CUP patients and their families is scarce, this study aimed to compare the family-perceived burden of CUP with that of common cancers (lung, colon, and stomach cancer). Additional aims were to explore the association between family-perceived burden and CUP patients’ quality of life (QOL) at end-of-life and family depression.Methods: This was a pre-planned secondary analysis of nationwide cross-sectional survey data from the bereaved family members of patients with cancer who died at 286 institutions. The major measurements were the eight-item family-perceived Burden scale (comprising specialist access, uncertainty, and prolonged diagnosis), Good Death Inventory, and Patient Health Questionnaire 9.Results: Of the total 27,591 survey responses, we analyzed 97 and 717 responses from the family members of patients with CUP and common cancer, respectively. The families of CUP patients scored significantly higher on all three burden subscales than those of common cancer patients (effect sizes: specialist access subscale, 0.3; uncertainty subscale, 0.66; and prolonged diagnosis subscale, 0.69; adjusted P < 0.01). Greater family Burden was significantly associated with lower patient QOL and higher family depression. Burden was significantly associated with being a spouse, second opinion consultation, and diagnosis period of >1 month.Conclusion: The families of CUP patients experience poor specialist access, greater uncertainty, and a prolonged diagnosis. They should be cared for from the initial stages to establish access to specialists, obtain an early diagnosis, and reduce uncertainty.
Residue Regulating Homotopy Method for Strongly Nonlinear Oscillator
Authors: Song Penghui , Zhang Wenming , Shao Lei
Published: January 2022

It is highly desired yet challenging to obtain analytical approximate solutions to strongly nonlinear oscillators accurately and efficiently. Here we propose a new approach, which combines the homtopy concept with a “residue-regulating” technique to construct a continuous homotopy from an initial guess solution to a high-accuracy analytical approximation of the nonlinear problems, namely the residue regulating homotopy method (RRHM). In our method, the analytical expression of each order homotopy-series solution is associated with a set of base functions which are pre-selected or generated during the previous order of approximations, while the corresponding coefficients are solved from deformation equations specified by the nonlinear equation itself and auxiliary residue functions. The convergence region, rate and final accuracy of the homotopy are controlled by a residue-regulating vector and an expansion threshold. General procedures of implementing RRHM are demonstrated using the Duffing and Van der Pol-Duffing oscillators, where approximate solutions containing abundant frequency components are successfully obtained, yielding significantly better convergence rate and performance stability compared to the other conventional homotopy-based methods.
Grinding Performance Integrated Experimental Evaluation on Alumina Ceramics with Leaf-Vein Bionic Grinding Wheel
Authors: Li Chao , Duan Jia , Xiaohong Zhang , Shi Zhaoyao , Yuchi Guangzhi , Zhang Songhui , Li Wei , Wen Dongdong , Luo Shi
Published: January 2022

In order to enhance the grinding performance of alumina ceramic materials, the surface of the grinding wheels are ablated by laser radiation before grinding, and the three types of leaf-vein bionic grinding wheels with different micro-groove pitches are formed to compare the grinding experiments with normal grinding wheels. The grinding forces and surface roughness were gauged and the morphological characteristics of ground workpiece surfaces were studied. The results showed that with the increase of groove pitch, the normal grinding force was reduced by 9.6-63%, while the tangential grinding force is reduced by 8.3-42%. The groove can promote the flow of coolant, accelerate the heat dissipation and chip removal in the grinding area, reduce the damage of the workpiece and the wear of the grinding wheel, so the vein bionic grinding wheel had more tremendous processing advantages. Among the four kinds of grinding wheels, the leaf-vein bionic grinding wheel with groove pitch equal to 8mm obtained the best grinding effect. The vein-shaped groove had a positive impact on the grinding process.
10 -Years Coronary Artery Disease Incidence and its Predictors in Overweight and Obese Adults Using A Competing Risk Model: A Longitudinal Study of Yazd Healthy Heart Cohort (YHHC)
Authors: Shahi‎ Mohsen Askari , Namayandeh Seyedeh Mahdieh , Midbody Mahmood Emami , Majidpour Fatemeh
Published: January 2022

We applied competing risk model to identify the predictors for Coronary Artery Disease (CAD) among 866 overweight and obese participants aged 20-74 years using their registered medical records in the first and second phase of Healthy Heart Cohort (YHHC) conducted in Yazd. These participants were free of coronary heart disease in the first phase of study. CAD was considered as the primary event and all other noncardiac deaths were considered as a competing event. The cumulative incidence of any CAD at the 5-year and 10-year follow-ups was approximately 6.8% and 10.6%, respectively, and approximately 4.6% and 8.5%, respectively, for all other noncardiac deaths. In both cause-specific and Fine-Gray models of risk factor diabetes type II, hypertriglyceridemia, university level of education (reversely), uric acid, age, systolic blood pressure and female gender (reversely) were associated with the increase risk of CAD. In addition to other traditional cardio metabolic risk factor we found that uric acid increased the risk of CAD in overweight and obese adults. It seems that lifestyle modification can reduce the risk of CAD. Also, high level of education had a protective effect on the risk of CAD. Both cause-specific and fine-gray models predicted similarly 10-years of CAD. The use of competing risk models in the presence of competing events is emphasized when interpreting survival studies.
Brg1 Promotes Airway Mucus Hypersecretion via IL-13 and the JAK1/2-STAT6 Signaling Pathway in Asthma
Authors: Zou Wenjing , xu maozhu , Hu Jie , Yang Lili , Gen Gang , Fu Zhou , Luo Zhengxiu
Published: January 2022

Backgroud: The chromatin remodeling factor Brg1 (Brahma-related gene 1) is an important nuclear protein that promotes the transcriptional activation or inhibition of target genes by regulating ATP hydrolysis to generate energy which rearranges the position of nucleosomes and the interaction of histone DNA. In this study, we explored the effect of Brg1 on airway mucus hypersecretion in asthma.Methods: Six-to-eight-week-old female wild-type C57BL/6 mice (wild-type, WT) and type II alveolar epithelial cells (AECIIs) specifically knockout Brg1 mice (Brg1fl/fl) were selected as the experimental subjects. The asthma group was established with house dust mite (HDM), and the control group was treated with normal saline (n=10). Wright's staining was used to detect inflammatory cells in bronchoalveolar lavage fluid (BALF). Invasive lung function was used to assess the airway compliance. Hematoxylin and eosin and periodic acid-schiff staining were used to detect mucus secretion. The virus was used to knock down the Brg1 gene in the bronchial epithelial cell line (16HBE) and stimulated with HDM. Immunohistochemistry was used to measure mucin glycoprotein 5AC (MUC5AC) protein expression in the airway epithelium and 16HBE cells. Western blotting was used to detect the expression of the MUC5AC and JAK1/2-STAT6 signaling pathways in mouse lung tissue and 16HBE. Co-immunoprecipitation (Co-IP) and Chromatin Immunoprecipitation (CHIP) were used to detect whether Brg1 could regulate the JAK1/2-STAT6 signaling pathway.Results: Specifically, knocking out the Brg1 gene in AECIIs can reduce airway inflammation, airway compliance, and mucus hypersecretion in asthma. Knockdown of the Brg1 gene can simultaneously reduce Interleukin-13 (IL-13) and the expression of MUC5AC protein in airway epithelial cells and the activation of the JAK1/2-STAT6 signaling pathway. The results of Co-IP and CHIP showed that Brg1 could bind to the JAK1/2 promoter region, regulating the activity of the JAK1/2-STAT6 pathway affects airway mucus secretion in asthma.Conclusion: Brg1 gene knockout in airway epithelial cells can reduce asthmatic airway mucus hypersecretion and the expression of MUC5AC protein in airway epithelial cells partly by inhibiting the activation of the JAK1/2-STAT6 signaling pathway.
Nano Zinc-Oxide Enhanced Photosynthetic Apparatus and Photosystem Efficiency of Maize (Zea Mays L.) in Sandy-Acidic Soils
Authors: Ahmad Wiqar , Nepal Jaya , Xin Xiaoping , He Zhenli
Published: January 2022

Conventional Zinc (Zn) fertilization (e.g., zinc sulfate) often leads to poor availability in soils. Zinc oxide nanoparticles (nano ZnO) can be a potential solution, but their effect on crop photosynthetic activity isn’t well documented. The effects of nano ZnO (50, 100, 150, 200 mg L-1) and application methods (seed-coating, soil-drench, and foliar-spray) in comparison with ZnSO4 recommended dose were evaluated for plant height, biomass, chlorophyll pigments and photosystem efficiency in a greenhouse pot experiment. 100 mg L-1 of nano ZnO significantly increased the chlorophyll (Chl.) a, b, a+b, carotenoids (x+c), a+b/x+c, SPAD, leaf Chl., total chlorophyll content plant-1, plant height and total biological yield (by 18-30%, 33-67%, 22-38%, 14-21%, 14-27%, 12-19%, 12-23% 58-99%, 6-11% and 16-20%, respectively) and reduced Chl. a/b (by 6-22%) over the other treatments (p<0.01) irrespective of application methods. Nano ZnO applied at 100 mg L-1 significantly increased photochemical quenching (qP) and efficiency of photosystem II (EPSII) compared to 150 and 200 mg L-1 regardless of application methods. The positive correlations between Chl. a and Chl. b (r2 0.90), Chl. a+b and x+c (r2=0.71), SPAD and Chl. a (r2=0.90), SPAD and Chl. b (r2=0.94) and SPAD and Chl. a+b (r2=0.93) indicates a uniform enhancement in chlorophyll pigments; SPAD value, qP, EPSII, and growth and yield parameters. This elucidates that the application of nano ZnO at 100 mg L-1 promotes corn biochemical health and photosynthesis, irrespective of the application method. These findings have a great propounding for improving plant growth through nano ZnO bio-fortification in acidic Spodosols.
Family Legacy: Depicting Diversity-Elevation Relationships of Tropical Tree Communities
Authors: Kamimura Vitor de Andrade , Marcusso Gabriel Mendes , Sabino Gabriel Pavan , Assis Marco Antonio , Joly Carlos Alfredo , Loiola Priscila de Paula
Published: January 2022

Unveiling the ecological processes driving diversity and its relationship to the environment remains a central goal in ecological studies. Here, we investigated the elevation effect on plant diversity patterns of tropical rainforests, using beta-, phylogenetic and alpha diversities. To do so, we compiled a forest dataset with 22,236 trees (DBH ≥ 4.8 cm) from 17 plots of 1 ha each along an elevational gradient (0 – 1,200 m a.s.l) in the Atlantic Forest of Southeastern Brazil. We found high phylogenetic and species rates of turnover – beta-diversity - along the elevational gradient. Alpha phylodiversity showed a monotonic decrease with increasing elevation, including or not fern species (a distantly related clade usually ignored in tropical ecology studies), while the phylogenetic structure was highly affected by the inclusion of fern trees. Species diversity showed a unimodal pattern for the whole community, and different patterns for the richest families. The diversity pattern of the whole community emerges from differences among species distribution of the richest families, while phylogenetic diversity seems to be gradually filtered by elevation. At intermediate elevations, higher species diversification within families might have led to different strategies and cooccurrence in tropical rainforests. We also showed that intricate effects of elevation in species assemblages can be better assessed using both ecological and evolutionary approaches, stressing the importance of species selection in diversity analyzes. Finally, we demonstrate that elevation has different effects on the species distributions of the richest families and warn that these differences should be considered in conservation planning.
Resveratrol Alleviates Oxidative Stress Induced by Oxidized Soybean Oils and Improves Gut Function via Changing Gut Microbiota in Weaned Piglets
Authors: Gao Yanan , Meng Qingwei , Song Xin , Zhao Qianqian , Shi Baoming
Published: January 2022

Background: The objective of this study was to investigate the effects of dietary resveratrol supplementation on growth performance, redox status, inflammatory state, and intestinal function of weaned piglets fed oxidized soybean oils.Methods: A total of twenty-eight castrated weaned male piglets with a similar body weight of 10.19 ±1 kg were randomly assigned to 4 dietary treatments for 28 days feeding trial with 7 replications per treatment and 1 piglet per replicate. Treatments were arranged as a 2×2 factorial with oil type [fresh soybean oils (FSOs) vs. oxidized soybean oils (OSOs)] and dietary resveratrol (RES) (0 vs. 300 mg/kg).Result: Inclusion of OSOs decreased the villus/crypt ratio (VCR), while the villus height (VH) and VCR in the jejunum of weaned piglets was increased by dietary RES (P< 0.05). The activities of lipase, chymotrypsin, and lactase were decreased by OSOs, however dietary RES supplementation increased the activities of lipase, chymotrypsin, lactase, and α-amylase in the jejunum of weaned piglets (P< 0.05). Dietary RES increased the apparent digestibility of crude fat (EE). Dietary RES supplementation in the diets supplemented with OSOs decreased the level of interleukin-1β (IL-1β), interleukin-6 (IL-6), interleukin-8 (IL-8), and tumor necrosis factor-α (TNF-α) in the plasma of weaned piglets, but failed to influence the IL-1β, IL-6, IL-8, and TNF-α level when diets supplemented with FSOs. Dietary RES alleviated the decrease of total-superoxide dismutase activity in the plasma of weaned piglets fed OSOs (P< 0.05). Dietary supplemented with OSOs and RES decreased the level of H2O2 in the plasma of weaned piglets (P< 0.05). RES alleviates the intestinal barrier damage fed OSOs in weaned piglets by increasing the mRNA expression of ZO-1 and Occludin. It is noteworthy that inclusion of OSOs in diets increased the abundance of Actinobacteria, and decreased the abundance of Tenercutes (P< 0.05). RES increased the abundance of Firmicutes, and decreased the abundance of Bacteroidetes (P< 0.05). At the genus level, RES decreased the abundance of Prevotella-1, Prevotellaceae UCG003, and Clostridium_sensu_stricto_6 in the colon. OSOs decreased the level of acetic acid, and dietary RES increased the level of acetic acid and butyric acid in the colon of weaned piglets.Conclusions: Dietary RES supplementation improved the villus-crypt structure, digestive enzyme activities and alleviated OSOs induced digestive absorption disorder. In addition, RES may alleviate OSOs immune status and energy metabolism of weaned piglets by affecting gut microbiota and its metabolite SCFAs. Notably, this positive effect of RES on OSOs may be related to decrease in the abundance of Prevotella_1 and Prevotellaceae_UCG-003.
WITHDRAWN: Prediction of Osteoporosis Through Deep Learning Algorithms on Panoramic Radiographs
Authors: Tassoker Melek , Ozic Muhammet Usame , Yuce Fatma
Published: January 2022

Objective: The aim of the present study was to predict osteoporosis on panoramic radiographs of women over 50 years of age through deep learning algorithms.Method: Panoramic radiographs of 744 female patients over 50 years of age were labeled as C1, C2, and C3 depending on mandibular cortical index (MCI). According to this index; C1: presence of a smooth and sharp mandibular cortex (normal); C2: resorption cavities at endosteal margin and 1 to 3-layer stratification (osteopenia); C3: completely porotic cortex (osteoporosis). The data of the present study were reviewed in different categories including C1-C2-C3, C1-C2, C1-C3 and C1-(C2+C3) as two-class and three-class prediction. The data were separated as 20% random test data; and the remaining data were used for training and validation with 5-fold cross-validation. AlexNET, GoogleNET, ResNET-50, SqueezeNET, and ShuffleNET deep learning models are trained through the transfer learning method. The results were evaluated by performance criteria including accuracy, sensitivity, specificity, F1-score, AUC and training duration. Findings: The dataset C1-C2-C3 has an accuracy rate of 81.14% with AlexNET; the dataset C1-C2 has an accuracy rate of 88.94% with GoogleNET; the dataset C1-C3 has an accuracy rate of 98.56% with AlexNET; and the dataset C1-(C2+C3) has an accuracy rate of 92.79% with GoogleNET. Conclusion: The highest accuracy was obtained in differentiation of C3 and C1 where osseous structure characteristics change significantly. Since the C2 score represent the intermediate stage (osteopenia), structural characteristics of the bone present behaviors closer to C1 and C3 scores. Therefore, the data set including the C2 score provided relatively lower accuracy results.
Complete Genome Sequence and Phylogenetic Analysis of a Goose Astrovirus Isolate in China
Authors: Tan ZHENG , Zhai Hui , Sun Ruqi , Xie Ruyu , Sun Zhe , Zhang Lilin , Li Zexing , huang jinhai
Published: January 2022

Astroviruses are considered the cause of gastroenteritis in humans and animals. Studies in recent years show avian astroviruses are also associated with duckling hepatitis, gosling gout, and chicken nephritis. In this study, a GAstV strain, designated as JS2019/China, was detected in dead goslings from a commercial goose farm in Jiangsu province of China. Viral strain was proliferated in goose embryos and sequence analysis showed the isolated strain had a classical structure arrangement and a series of conserved regions compared with other GAstVs. Sequence comparison and phylogenetic analysis of whole genome and ORF2 revealed that JS2019/China belongs to the GAstV group-1, which consists of most of the GAstV strains. Amino acid analysis indicated that some mutants might have an impact on viral protease capacity, such as V505I and K736E of ORF1a and T107I, F342S, and S606P of ORF2. Taken together, a novel GAstV strain was isolated and genomic analysis and protein polymorphism analysis indicated that some amino acid mutants might affect the viral virulence.
Changes in Tillering, Nutritional Status, and Biomass Yield of Panicum Maximum Used for Cadmium Phytoextraction
Authors: Rabêlo Flávio Henrique Silveira , Santos Felipe dos , Lavres José , Alleoni Luís
Published: January 2022

Although several grasses have been evaluated for cadmium (Cd) phytoextraction, there are no studies assessing how Cd is accumulated and distributed in the tissues of Panicum maximum grown in mildly polluted soils. The evaluation of tillering, nutritional status and biomass yield of this grass, mainly along successive shoot regrowths, is not well studied so far. Thus, P. maximum Jacq. cv. Massai was grown for two periods in an Oxisol presenting bioavailable Cd concentrations varying from 0.04 (control) to 10.91 mg kg−1 soil. Biomass yield of leaves and stems´ growth have decreased under the highest Cd exposure, but it did not occur in the regrowth period, indicating that Cd-induced toxicity is stronger in the early stages of development of P. maximum. The tillering was not compromised even the basal node presenting Cd concentrations higher than 100 mg kg−1 DW. We identified a restriction on Cd transport upwards from basal node, which was the main local of Cd accumulation. Apparently, P, K, Mg, S and Cu are involved in processes that restrict Cd translocation and confer high tolerance to Cd in P. maximum. The Cd-induced nutritional disorders did not negatively correlate with factors used to calculate phytoextraction efficiency. However, the nutritional adjustments of P. maximum to cope with Cd stress restricted the upward Cd transport, which decreased the phytoextraction efficiency from the available Cd concentration of 5.93 mg kg−1 soil.
Understanding Mentoring Style of Mentors and Science Identity Formation in Historically Excluded Undergraduate Students in Natural History Collections
Authors: Pirlo Jeanette
Published: January 2022

Background: Science identity formation is integral to the transformation from a student to a member of the STEM workforce. However, the formation of a student’s science identity is affected by various factors such as support from mentors and community members, the student’s perception of their peers’ acceptance of them, as well as the student’s own perception of their role in research. The aim of this study is to investigate mentors’ understanding of science identity formation, their mentoring style, and if it is effective when working with students from historically excluded populations. A phenomenological approach was used to conduct this study. Structured interviews were conducted with natural history collections faculty members of various backgrounds and career levels. Results: Three themes emerged that contribute to science identity formation through mentoring: 1. Factors for science identity formation; 2. Mentorship among different career stages, and 3. Defining own mentoring style. Predominantly, participants realized that their own experience as a mentee shapes how they approach mentoring.Conclusions: Although this study describes the mentee’s science identity formation from the mentor’s point of view, the study’s participants realized the importance they play in a student’s science identity formation, and that mindful mentoring is necessary for success when engaging historically excluded groups in the STEM workforce.
Effects of ultrasonic surface rolling process on tribological behavior of 4Cr13 stainless steel
Authors: Luo Xiaoshuang , Zhan Shengpeng , Jia Dan , Tu Jiesong , Li Yinhua , Yang Tian , Duan Haitao
Published: January 2022

Ultrasonic surface rolling (USR) process is a novel surface strengthening technique based on the tool head's high-frequency impact on the workpiece. USR can cause severe plastic deformation on the superficial surface of metal material, and greatly improving the mechanical properties of the material. This paper elucidates the effects of USR passes on the surface roughness, sample height, microstructure, microhardness, residual stress, and tribological properties of 4Cr13 stainless steel. The results revealed that multiple USR treatments refined the near-surface layer grain of the sample. Compared with untreated sample, USR treatments significantly improved the surface roughness and microhardness of the samples. Obvious compressive residual stress and plastic deformed with a maximum value of about -723 MPa and a depth of about 229 μm were also introduced into the sample surface. Under a dry friction environment, the samples that underwent the USR treatments exhibited significantly enhanced wear resistance, and six rolling passes were found to be the most suitable treatment.
Prospective Study on the Performance of 18F-DOPA Positron Emission Tomography/Computed Tomography in Patients with Medullary Thyroid Carcinoma
Authors: Califano Inés , Pitoia Fabian , Chirico Roxana , de Salazar Alejandra , Bastianello Maria
Published: January 2022

Purpose

18F-DOPA Positron Emission Tomography/Computed Tomography (18F-DOPA PET/CT) is a sensitive functional imaging method (65-75%) for detecting disease localization in medullary thyroid cancer (MTC). We aimed: i) to assess the clinical usefulness of 18F-DOPA PET/CT in patients with MTC and elevated calcitonin (Ctn) and CEA levels and, ii) to evaluate changes in disease management secondary to the findings encountered with this methodology.
Methods

thirty-six patients with MTC and Ctn levels ≥150 pg/ml were prospectively included. Neck ultrasound, chest contrast-enhanced CT, liver magnetic resonance imaging/ abdominal 3-phase contrast-enhanced CT and bone scintigraphy were carried out up to 6 months before the 18F DOPA PET/CT.
Results

77.7% were female and 27% had hereditary MTC. Median Ctn level was 1450 pg/ml [150-56620], median CEA level 413 ng/ml [2.9-7436]. Median Ctn DT was 37.5 months [5.7-240]; median CEA DT was 31.8 [4.9-180]. 18F-DOPA PET/CT was positive in 33 patients (91.6%); in 18 (56%) uptake was observed in lymph nodes in the neck or mediastinum, in 7 cases (22%) distant metastases were diagnosed, and in 8 additional patients (24%) both locoregional and distant sites of disease were found. Ctn and CEA levels were higher in patients with ≥ 3 foci of distant metastases. In 14 patients (38.8%), findings on 18F-DOPA PET/CT led to changes in management; surgery for locoregional lymph nodes was the most frequent procedure in 8 patients (22%).
Conclusion

18F-DOPA PET/CT was useful for the detection of recurrent disease in MTC and provided helpful information for patient management.
High expression of TMEM33 predicts poor prognosis and promotes cell proliferation in cervical cancer
Authors: Chen Hanxiang , Li Yongqing , Zhang Shaoming , Wang Yunshan , Wang Lili , Ma Wanshan
Published: January 2022

Background

As one of the most common cancer among women worldwide, the prognosis of patients with advanced cervical cancer remains unsatisfactory. A study indicated that transmembrane protein 33 (TMEM33) was implicated in tumor recurrence, while its role in cervical cancer has not been elucidated.
Methods

TMEM33 expression in cervical squamous cell carcinoma and endocervical adenocarcinoma (CESC) was primarily screened in The Cancer Genome Altas (TCGA), and further validated in Gene Expression Omnibus (GEO) database. The Kaplan–Meier plotter analysis and Cox regression were constructed to evaluate the prognostic value of TMEM33 in CESC. Functional enrichment analysis was performed with GO, KEGG and GSEA tools. Protein-protein interaction analysis and correlated gene networks were conducted using STING and GEPIA2 websites, respectively. The expression of TMEM33 in cervical cancer cells were examined by immunoblotting and RT-qPCR. Finally, CCK-8 assay and colony formation assay were performed to investigate the role of TMEM33 in cervical cancer cell proliferation.
Results

TMEM33 expression was significantly elevated in CESC compared with normal tissues. High expression of TMEM33 was associated with poor prognostic clinical characteristics in CESC patients. KM-plotter analysis revealed that patients with increased TMEM33 had shorter overall survival (OS), progress free interval (PFI), and disease specific survival (DSS). Moreover, Multivariate Cox analysis further confirmed that high TMEM33 expression was an independent risk factor for OS in patients with CESC. TMEM33 was associated with immune cell infiltration, and its expression was correlated with tumorigenesis-related genes RNF4, OCIAD1, TMED5, DHX15, MED28 and LETM1. More importantly, knockdown of TMEM33 in cervical cancer cells decreased the expression of those genes and inhibited cell proliferation.
Conclusions

Increased TMEM33 in cervical cancer can serve as an independent prognostic marker and might play a role in tumorigenesis by promoting cell proliferation.
Prevalence of Posttraumatic Stress Disorder and Depression Among Internally Displaced Persons In Mogadishu
Authors: Ali Mustafa , Mutavi Teresia , Mathai Muthoni , Mburu John
Published: January 2022

Background

Nearly three decades of conflict and frequent droughts and environmental hardships, have left 2.6 million of Somalis in displacement camps. Even though psychological impact of war and natural disasters are well documented, little is known about the unseen scars of psychological trauma in Internally displaced persons in Somalia. The purpose of the study was to determine the prevalence of post-traumatic stress disorder (PTSD) and depression amongst internally displaced persons (IDPs), and examine association between displacement and these psychiatric conditions.
Methodology

A cross-sectional quantitative study was conducted among 406 IDPs in Mogadishu. Harvard Trauma Questionnaire was used to determine levels of trauma exposure and PTSD, and Hopkins Symptom Checklist-25 was used to estimate prevalence of depression. Multivariate and bivariate analysis was conducted to analyze the association of demographic and displacement variables on the outcomes of PTSD and depression.
Results

More than half (59%) of participants met the symptom criteria of depression, and nearly one third (32%) of respondents met the symptom criteria for PTSD. The most prevalent traumatic event was lack of food or water (80.2%). Important predictive factors in development of psychiatric morbidity were unemployment, cumulative traumatic exposure, frequency and duration of displacement.
Conclusion

The study revealed high levels of Depressive disorder and Post-Traumatic Stress Disorder among internally displaced persons in Mogadishu. Furthermore, this study provided evidence to IDPs’ susceptibility to trauma exposure and lack of essential services and goods. Study also highlighted the importance of provision of Mental Health and Psychosocial Support (MHPSS) service in IDP camps.
Ferroptosis-Related lncRNA Signature Predicts Prognosis and Immunotherapy Efficacy in Cutaneous Melanoma
Authors: Xu Yujian , Chen Youbai , Niu Zehao , Yang Zheng , Xing Jiahua , Yin Xiangye , Guo Lingli , Zhang Qixu , Yang Yi , Han Yan
Published: January 2022

Ferroptosis-related lncRNAs are promising biomarkers for predicting the prognosis of many cancers. However, a ferroptosis-related signature to predict the prognosis of cutaneous melanoma (CM) has not been identified. The purpose of our study was to construct a ferroptosis-related lncRNA signature to predict prognosis and immunotherapy efficacy in CM. Ferroptosis-related differentially expressed genes (FDEGs) and lncRNAs (FDELs) were identified using TCGA, GTEx, and FerrDb datasets. We performed Cox and LASSO regressions to identify key FDELs, and constructed a risk score to stratify patients into high- and low-risk groups. A nomogram was developed for clinical use. We performed gene set enrichment analyses (GSEA) to identify significantly enriched pathways. Differences in the tumor microenvironment (TME) between the 2 groups were assessed using 7 algorithms. To predict the efficacy of immune checkpoint inhibitors (ICI), we analyzed the association between PD1 and CTLA4 expression and the risk score. Finally, differences in Tumor Mutational Burden (TMB) and molecular drugs Sensitivity between the 2 groups were performed. Here, we identified 5 lncRNAs (AATBC, AC145423.2, LINC01871, AC125807.2, and AC245041.1) to construct the risk score. The AUC of the lncRNA signature was 0.743 in the training cohort and was validated in the testing and entire cohorts. Kaplan-Meier analyses revealed that the high-risk group had poorer prognosis. Multivariate Cox regression showed that the lncRNA signature was an independent predictor of OS with higher accuracy than traditional clinicopathological features. The 1-, 3-, and 5-year survival probabilities for CM patients were 92.7%, 57.2%, and 40.2% with an AUC of 0.804, indicating a good accuracy and reliability of the nomogram. GSEA showed that the high-risk group had lower ferroptosis and immune response. TME analyses confirmed that the high-risk group had lower immune cell infiltration (e.g., CD8+ T cells, CD4+ memory-activated T cells, and M1 macrophages) and lower immune functions (e.g., immune checkpoint activation). Low-risk patients whose disease expressed PD1 or CTLA4 were likely to respond better to ICIs. The analysis demonstrated that the TMB had significantly difference between low- and high- risk groups. Chemotherapy drugs, such as sorafenib, Imatinib, ABT.888 (Veliparib), Docetaxel, and Paclitaxel showed Significant differences in the estimated IC50 between the two risk groups. Overall, our novel ferroptosis-related lncRNA signature was able to accurately predict the prognosis and ICI outcomes of CM patients. These ferroptosis-related lncRNAs might be potential biomarkers and therapeutic targets for CM.