bluuebunny/crossref_metadata_2025 huggingface dataset:
Sensitivity Analysis of Oxide Scale Influence on General Carbon Steels during Hot Forging
Authors: Behrens Bernd-Arno , Chugreev Alexander , Awiszus Birgit , Graf Marcel , Kawalla Rudolf , Ullmann Madlen , Korpala Grzegorz , Wester Hendrik
Published: February 2018

Increasing product requirements have made numerical simulation into a vital tool for the time- and cost-efficient process design. In order to accurately model hot forging processes with finite, element-based numerical methods, reliable models are required, which take the material behaviour, surface phenomena of die and workpiece, and machine kinematics into account. In hot forging processes, the surface properties are strongly affected by the growth of oxide scale, which influences the material flow, friction, and product quality of the finished component. The influence of different carbon contents on material behaviour is investigated by considering three different steel grades (C15, C45, and C60). For a general description of the material behaviour, an empirical approach is used to implement mathematical functions for expressing the relationship between flow stress and dominant influence variables like alloying elements, initial microstructure, and reheating mode. The deformation behaviour of oxide scale is separately modelled for each component with parameterized flow curves. The main focus of this work lies in the consideration of different materials as well as the calculation and assignment of their material properties in dependence on current process parameters by application of subroutines. The validated model is used to carry out the influence of various oxide scale parameters, like the scale thickness and the composition, on the hot forging process. Therefore, selected parameters have been varied within a numerical sensitivity analysis. The results show a strong influence of oxide scale on the friction behaviour as well as on the material flow during hot forging.
Role of Volcano-Sedimentary Basins in the Formation of Greenstone-Granitoid Belts in the West African Craton: A Numerical Model
Authors: Feng Xiaojun , Wang Enyuan , Ganne Jérôme , Amponsah Prince , Martin Roland
Published: February 2018

Greenstone belts in the West African Craton (WAC) are separated by several generations of granitoids intruded at ca. 2.18–1.98 Ga. Simultaneous folding and exhumation play an important role in the formation of greenstone-granitoid belts. However, the overall tectonic regime and origin of granitoids remain controversial. In this study, we present the estimates of the mantle potential temperature (Tp) for the WAC, which yields values of about 1500–1600 °C, pressure estimates of initial and final melting yield values of about 3.7–5.2 GPa and 1–1.3 GPa, respectively. Subsequently, 2D thermo-mechanical models have been constructed to explore the width of volcano-sedimentary basin on spatial-temporal evolution of diapirs that emplaced in the lower-middle crust during compression. The models show that the width of the volcano-sediment layer plays an important role in the formation mechanisms of greenstone-granitoid belts. The lower crust beneath sedimentary sequences is deformed into a buckle fold during the first compressional stage, through which relief uplifts slowly. Subsequently, the buckle fold is further deformed into several individual folds. Diapirs made of lower crust rocks ascend and emplace in the middle-upper crust resulting from instability. Benefitting from the mantle temperature, the pressure estimates and the numerical modelling results, a new geodynamic model was constructed. This model indicates that a series of sheet-like granitoids possibly derived from either subducted mélanges, lower crust and/or mantle melting that are accumulated at depths of the subcontinental mantle would channel along diapirs before feeding the upper crust. When the granitoids arrive at the solidified lids of the diapirs, they would favour migrating horizontally and intrude into the upper crust through weakening zones between the diapirs. Our geodynamic model also suggests an asymmetry of structures between the upper and middle-lower crust, with the dome-like granitoids overlying high-grade sedimentary synforms and high-grade diapirs underlying low-grade greenstone belts.
Chemical Composition of Pyroligneous Acid Obtained from Eucalyptus GG100 Clone
Authors: Pimenta Alexandre , Fasciotti Maíra , Monteiro Thays , Lima Kássio
Published: February 2018

The present study aimed to characterize the chemical composition of pyroligneous acid (PA) obtained from slow pyrolysis of the clone GG100 of Eucalyptus urophylla × Eucalyptus grandis. The efficiency of extraction of organic compounds by using different solvents—dichloromethane (DCM), diethyl ether (DE) and ethyl acetate (EA)—was evaluated. Wood discs were collected and carbonized at a heating rate of 1.25 °C/min until 450 °C. Pyrolysis gases were trapped and condensed, yielding a crude liquid product (CLP), which was refined to obtain pure PA. Then liquid–liquid extraction was carried out. Each extracted fraction was analyzed by GC-MS and the chemical compounds were identified. Experimental results showed that a larger number of chemical compounds could be extracted by using DCM and EA in comparison to diethyl ether DE. A total number of 93 compounds were identified, with phenolic compounds being the major group, followed by aldehydes and ketones, furans, pyrans and esters. Higher contents of guaiacol, phenol, cresols and furfural seem to explain the antibacterial and antifungal activity shown by PA, as reported previously in the literature. Experimental data indicated that the organic phase extracted from GG100 PA consists of a mixture of compounds similar to liquid smokes regularly used in the food industry.
Organ-Specific Metabolic Shifts of Flavonoids in Scutellaria baicalensis at Different Growth and Development Stages
Authors: Xu Jingyuan , Yu Yilan , Shi Ruoyun , Xie Guoyong , Zhu Yan , Wu Gang , Qin Minjian
Published: February 2018

Scutellaria baicalensis Georgi is a traditional Chinese herbal medicine mainly containing flavonoids that contribute to its bioactivities. In this study, the distributions and dynamic changes of flavonoid levels in various organs of S. baicalensis at different development stages were investigated by UHPLC-QTOF-MS/MS and HPLC-DAD methods. The results indicated that the metabolic profiles of S. baicalensis changed with growth and development. During the initial germination stage, the seeds mainly contained flavonols. With growth, the main kinds of flavonoids in S. baicalensis changed from flavonols to flavanones and flavones. The results also revealed that the accumulation of flavonoids in S. baicalensis is organ-specific. The flavones without 4′-OH groups mainly accumulate in the root and the flavanones mainly accumulate in aerial organs. Dynamic accumulation analysis showed that the main flavonoids in the root of S. baicalensis accumulated rapidly before the full-bloom stage, then changed to a small extent. The results suggested the proper harvest time for the aerial parts was at the initial stage of reproductive growth and the flower buds should be collected before flowering. This study deepening the knowledge of S. baicalensis should provide valuable information for guiding the scientific cultivation of this plant and the development and utilization of S. baicalensis.
Inhibition of Cytochrome P450 Activities by Extracts of Hyptis verticillata Jacq.: Assessment for Potential HERB-Drug Interactions
Authors: Picking David , Chambers Bentley , Barker James , Shah Iltaf , Porter Roy , Naughton Declan , Delgoda Rupika
Published: February 2018

Understanding the potential for adverse drug reactions (ADRs), from herb-drug interactions, is a key aspect of medicinal plant safety, with particular relevance for public health in countries where medicinal plant use is highly prevalent. We undertook an in-depth assessment of extracts of Hyptis verticillata Jacq., via its impact on activities of key cytochrome P450 (CYP) enzymes (CYPs 1A1, 1A2, 1B1, 3A4 and 2D6), its antioxidant properties (determined by DPPH assays) and chemical characterisation (using LC-MS). The dried plant aqueous extract demonstrated potent inhibition of the activities of CYPs 1A1 (7.6 µg/mL), 1A2 (1.9 µg/mL), 1B1 (9.4 µg/mL) and 3A4 (6.8 µg/mL). Further analysis of other crude extracts demonstrated potent inhibition of CYP1A2 activity for a dried plant ethanol extract (1.5 µg/mL), fresh plant ethanol extract (3.9 µg/mL), and moderate activity for a fresh plant aqueous extract (27.8 µg/mL). All four extracts demonstrated strong antioxidant activity, compared to the positive control (ascorbic acid, 1.3 µg/mL), with the dried plant ethanol extract being the most potent (1.6 µg/mL). Analysis of the dried plant aqueous extract confirmed the identity of seven phytochemicals, five lignans and two triterpenes. Individual screening of these phytochemicals against the activity of CYP1A2 identified yatein as a moderate inhibitor (71.9 μM), likely to contribute to the plant extract’s potent bioactivity. Further analysis on the impact of this plant on key drug metabolizing enzymes in vivo appears warranted for likely ADRs, as well as furthering development as a potential chemopreventive agent.
The Lewis Pair Polymerization of Lactones Using Metal Halides and N-Heterocyclic Olefins: Theoretical Insights
Authors: Meisner Jan , Karwounopoulos Johannes , Walther Patrick , Kästner Johannes , Naumann Stefan
Published: February 2018

Lewis pair polymerization employing N-Heterocyclic olefins (NHOs) and simple metal halides as co-catalysts has emerged as a useful tool to polymerize diverse lactones. To elucidate some of the mechanistic aspects that remain unclear to date and to better understand the impact of the metal species, computational methods have been applied. Several key aspects have been considered: (1) the formation of NHO-metal halide adducts has been evaluated for eight different NHOs and three different Lewis acids, (2) the coordination of four lactones to MgCl2 was studied and (3) the deprotonation of an initiator (butanol) was investigated in the presence and absence of metal halide for one specific Lewis pair. It was found that the propensity for adduct formation can be influenced, perhaps even designed, by varying both organic and metallic components. Apart from the NHO backbone, the substituents on the exocyclic, olefinic carbon have emerged as interesting tuning site. The tendency to form adducts is ZnCl2 > MgCl2 > LiCl. If lactones coordinate to MgCl2, the most likely binding mode is via the carbonyl oxygen. A chelating coordination cannot be ruled out and seems to gain importance upon increasing ring-size of the lactone. For a representative NHO, it is demonstrated that in a metal-free setting an initiating alcohol cannot be deprotonated, while in the presence of MgCl2 the same process is exothermic with a low barrier.
Complete Chloroplast Genomes of Papaver rhoeas and Papaver orientale: Molecular Structures, Comparative Analysis, and Phylogenetic Analysis
Authors: Zhou Jianguo , Cui Yingxian , Chen Xinlian , Li Ying , Xu Zhichao , Duan Baozhong , Li Yonghua , Song Jingyuan , Yao Hui
Published: February 2018

Papaver rhoeas L. and P. orientale L., which belong to the family Papaveraceae, are used as ornamental and medicinal plants. The chloroplast genome has been used for molecular markers, evolutionary biology, and barcoding identification. In this study, the complete chloroplast genome sequences of P. rhoeas and P. orientale are reported. Results show that the complete chloroplast genomes of P. rhoeas and P. orientale have typical quadripartite structures, which are comprised of circular 152,905 and 152,799-bp-long molecules, respectively. A total of 130 genes were identified in each genome, including 85 protein-coding genes, 37 tRNA genes, and 8 rRNA genes. Sequence divergence analysis of four species from Papaveraceae indicated that the most divergent regions are found in the non-coding spacers with minimal differences among three Papaver species. These differences include the ycf1 gene and intergenic regions, such as rpoB-trnC, trnD-trnT, petA-psbJ, psbE-petL, and ccsA-ndhD. These regions are hypervariable regions, which can be used as specific DNA barcodes. This finding suggested that the chloroplast genome could be used as a powerful tool to resolve the phylogenetic positions and relationships of Papaveraceae. These results offer valuable information for future research in the identification of Papaver species and will benefit further investigations of these species.
Preparation of Novel Homodimers Derived from Cytotoxic Isoquinolinequinones. A Twin Drug Approach
Authors: Ibacache Juana , Faundes Judith , Montoya Margarita , Mejías Sophia , Valderrama Jaime
Published: February 2018

The synthesis of five novel homodimers is reported based on the anilinoisoquinolinequinone scaffold. In these twin-drug derivatives, two units of the anilinoquinone pharmacophores are linked through a methylene spacer. The formation of dimers was achieved by reaction of isoquinolinequinones with 4, 4’-diaminodiphenylmethane via a sequence of two oxidative amination reactions. A preliminary in vitro screening of the homodimers reveals moderate to high cytotoxic activities against MDA-MB-21 breast adenocarcinoma and B16-F10 murine metastatic melanoma cell lines. The asymmetrical homodimer 15 stands out due to its cytotoxic potencies at submicromolar concentrations and high selectivity index (mean IC50 = 0.37 μM; SI = 6.97) compared to those of etoposide (mean IC50 = 3.67; SI = 0.32) and taxol (mean IC50 = 0.35; SI = 0.91) employed as reference anticancer drugs.
Food-Grade Encapsulation Systems for (−)-Epigallocatechin Gallate
Authors: Shi Meng , Shi Yun-Long , Li Xu-Min , Yang Rui , Cai Zhuo-Yu , Li Qing-Sheng , Ma Shi-Cheng , Ye Jian-Hui , Lu Jian-Liang , Liang Yue-Rong , Zheng Xin-Qiang
Published: February 2018

(−)-Epigallocatechin gallate (EGCG) has attracted significant research interest due to its health-promoting effects such as antioxidation, anti-inflammation and anti-cancer activities. However, its instability and poor bioavailability have largely limited its efficacy and application. Food-grade materials such as proteins, carbohydrates and lipids show biodegradability, biocompatibility and biofunctionality properties. Food-grade encapsulation systems are usually used to improve the bioavailability of EGCG. In the present paper, we provide an overview of materials and techniques used in encapsulating EGCG, in which the adsorption mechanisms of food-grade systems during in vitro digestion are reviewed. Moreover, the potential challenges and future work using food-grade encapsulates for delivering EGCG are also discussed.
New Bacterial Phytase through Metagenomic Prospection
Authors: Farias Nathálya , Almeida Isabela , Meneses Carlos
Published: February 2018

Alkaline phytases from uncultured microorganisms, which hydrolyze phytate to less phosphorylated myo-inositols and inorganic phosphate, have great potential as additives in agricultural industry. The development of metagenomics has stemmed from the ineluctable evidence that as-yet-uncultured microorganisms represent the vast majority of organisms in most environments on earth. In this study, a gene encoding a phytase was cloned from red rice crop residues and castor bean cake using a metagenomics strategy. The amino acid identity between this gene and its closest published counterparts is lower than 60%. The phytase was named PhyRC001 and was biochemically characterized. This recombinant protein showed activity on sodium phytate, indicating that PhyRC001 is a hydrolase enzyme. The enzymatic activity was optimal at a pH of 7.0 and at a temperature of 35 °C. β-propeller phytases possess great potential as feed additives because they are the only type of phytase with high activity at neutral pH. Therefore, to explore and exploit the underlying mechanism for β-propeller phytase functions could be of great benefit to biotechnology.
Electronic Structure of C60/Zinc Phthalocyanine/V2O5 Interfaces Studied Using Photoemission Spectroscopy for Organic Photovoltaic Applications
Authors: Lim Chang Jin , Park Min Gyu , Kim Min Su , Han Jeong Hwa , Cho Soohaeng , Cho Mann-Ho , Yi Yeonjin , Lee Hyunbok , Cho Sang Wan
Published: February 2018

The interfacial electronic structures of a bilayer of fullerene (C60) and zinc phthalocyanine (ZnPc) grown on vanadium pentoxide (V2O5) thin films deposited using radio frequency sputtering under various conditions were studied using X-ray and ultraviolet photoelectron spectroscopy. The energy difference between the highest occupied molecular orbital (HOMO) level of the ZnPc layer and the lowest unoccupied molecular orbital (LUMO) level of the C60 layer was determined and compared with that grown on an indium tin oxide (ITO) substrate. The energy difference of a heterojunction on all V2O5 was found to be 1.3~1.4 eV, while that on ITO was 1.1 eV. This difference could be due to the higher binding energy of the HOMO of ZnPc on V2O5 than that on ITO regardless of work functions of the substrates. We also determined the complete energy level diagrams of C60/ZnPc on V2O5 and ITO.
Precision Aliphatic Polyesters via Segmer Assembly Polymerization
Authors: Zeng Fu-Rong , Liang Yang , Li Zi-Long
Published: February 2018

Precise structure-property relation of a biodegradable polymer (e.g., aliphatic polyester) is anticipated only if monomer units and chiral centers are arranged in a defined primary sequence as a biomacromolecule. An emerging synthetic methodology, namely segmer assembly polymerization (SAP), is introduced in this paper to reveal the latest progress in polyester synthesis. Almost any periodic polyester envisioned can be synthesized via SAP using a programed linear or cyclic monomer. In this context, the macroscopic properties of a biodegradable polymer are fundamentally determined by microstructural information through a bottom-up approach. It can be highlighted that SAP ideally combines the precision of organic synthesis and the high efficiency of a polymerization reaction. Previously reported strategies including nucleophilic displacement, polyesterification, cross-metathesis polymerization (CMP), ring-opening polymerization (ROP), ring-opening metathesis polymerization (ROMP) and entropy-driven ring-opening metathesis polymerization (ED-ROMP) are critically reviewed in this paper to shed light on precision synthesis of aliphatic polyesters via SAP. Emerging yet challenging, SAP is a paradigm which reflects the convergence of organic and polymer chemistries and is also an efficient pathway to microstructural control. The current status, future challenges and promising trends in this realm are analyzed and discussed in this overview of the state-of-the-art.
Identification of Novel Protein Kinase Receptor Type 2 Inhibitors Using Pharmacophore and Structure-Based Virtual Screening
Authors: Cruz Josiane , Neto Moysés , Silva Luciane , Da S. Ramos Ryan , Da S. Costa Josivan , Brasil Davi , Lobato Cleison , Da Costa Glauber , Bittencourt José , Da Silva Carlos , Leite Franco , Santos Cleydson
Published: February 2018

The Protein Kinase Receptor type 2 (RIPK2) plays an important role in the pathogenesis of inflammatory diseases; it signals downstream of the NOD1 and NOD2 intracellular sensors and promotes a productive inflammatory response. However, excessive NOD2 signaling has been associated with various diseases, including sarcoidosis and inflammatory arthritis; the pharmacological inhibition of RIPK2 is an affinity strategy that demonstrates an increased expression of pro-inflammatory secretion activity. In this study, a pharmacophoric model based on the crystallographic pose of ponatinib, a potent RIPK2 inhibitor, and 30 other ones selected from the BindingDB repository database, was built. Compounds were selected based on the available ZINC compounds database and in silico predictions of their pharmacokinetic, toxicity and potential biological activity. Molecular docking was performed to identify the probable interactions of the compounds as well as their binding affinity with RIPK2. The compounds were analyzed to ponatinib and WEHI-345, which also used as a control. At least one of the compounds exhibited suitable pharmacokinetic properties, low toxicity and an interesting binding affinity and high fitness compared with the crystallographic pose of WEHI-345 in complex with RIPK2. This compound also possessed suitable synthetic accessibility, rendering it a potential and very promising RIPK2 inhibitor to be further investigated in regards to different diseases, particularly inflammatory ones.
Inhibitory Influence of Panax notoginseng Saponins on Aspirin Hydrolysis in Human Intestinal Caco-2 Cells
Authors: Sun Zongxi , Wu Yali , Yang Bing , Zhu Baochen , Hu Shaonan , Lu Yang , Zhao Bo , Du Shouying
Published: February 2018

Herb-drug interactions are important safety concerns in clinical practice. The interactions occur firstly in the intestinal absorption for orally administered drugs. Aspirin and Panax notoginseng saponins (PNS)-based drugs are often combined in China to prevent larger-artery atherosclerosis. Here, we aimed to characterize the aspirin transport across Caco-2 cell monolayers, a model of the intestinal absorption, and further to evaluate the influence of PNS on aspirin hydrolysis and the relating mechanisms. Transcellular transport of aspirin and the influence of PNS were explored using Caco-2 cell monolayers. The protein expression of human carboxylesterase 1 (hCE1) and hCE2 in Caco-2 cells after PNS treatment was analyzed by ELISA, and the mRNA level were determined by qRT-PCR. In the study, Caco-2 cells showed high level of hydrolase activity, and most aspirin was hydrolyzed inside the cells during the transport process. Interestingly, PNS were demonstrated to inhibit the esterase activities responsible for aspirin hydrolysis in Caco-2 cells. PNS could also decrease the protein expression of hCE1 and hCE2, whereas exhibited minor effect on the mRNA expression. These results indicated that oral administration of PNS-based drugs might inhibit the hydrolysis of aspirin during intestinal absorption thus promoting its bioavailability.
Radical Polymerization of Alkyl 2-Cyanoacrylates
Authors: Duffy Cormac , Zetterlund Per B. , Aldabbagh Fawaz
Published: February 2018

Cyanoacrylates (CAs) are well-known fast-setting adhesives, which are sold as liquids in the presence of stabilizers. Rapid anionic polymerization on exposure to surface moisture is responsible for instant adhesion. The more difficult, but synthetically more useful radical polymerization is only possible under acidic conditions. Recommendations on the handling of CAs and the resulting polymers are provided herein. In this review article, after a general description of monomer and polymer properties, radical homo- and copolymerization studies are described, along with an overview of nanoparticle preparations. A summary of our recently reported radical polymerization of CAs, using reversible addition-fragmentation chain transfer (RAFT) polymerization, is provided.
Small-Sized Mg–Al LDH Nanosheets Supported on Silica Aerogel with Large Pore Channels: Textural Properties and Basic Catalytic Performance after Activation
Authors: Wang Lijun , Wang Yusen , Wang Xiaoxia , Feng Xiaolan , Ye Xiao , Fu Jie
Published: February 2018

Layered double hydroxides (LDHs) have been widely used as an important subset of solid base catalysts. However, developing low-cost, small-sized LDH nanoparticles with enhanced surface catalytic sites remains a challenge. In this work, silica aerogel (SA)-supported, small-sized Mg–Al LDH nanosheets were successfully prepared by one-pot coprecipitation of Mg and Al ions in an alkaline suspension of crushed silica aerogel. The supported LDH nanosheets were uniformly dispersed in the SA substrate with the smallest average radial diameter of 19.2 nm and the thinnest average thickness of 3.2 nm, both dimensions being significantly less than those of the vast majority of LDH nanoparticles reported. The SA/LDH composites also showed large pore volume (up to 1.3 cm3·g) and pore diameter (>9 nm), and therefore allow efficient access of reactants to the edge catalytic sites of LDH nanosheets. In a base-catalyzed Henry reaction of benzaldehyde with nitromethane, the SA/LDH catalysts showed high reactant conversions and favorable stability in 6 successive cycles of reactions. The low cost of the SA carrier and LDH precursors, easy preparation method, and excellent catalytic properties make these SA/LDH composites a competitive example of solid-base catalysts.
Impact of Selenium Addition to Animal Feeds on Human Selenium Status in Serbia
Authors: Pavlovic Zoran , Miletic Ivanka , Zekovic Milica , Nikolic Marina , Glibetic Maria
Published: February 2018

Research conducted during the 1980s demonstrated Se deficiency in humans. Increased inclusion of selenium in animal feeds started from the year 2000 onwards. The aim of this study was to estimate the effects of selenium inclusion in animal feeds on human selenium status and dietary habits of the Serbian population related to food of animal origin. Plasma selenium concentration in healthy adult volunteers, including residents of one of the regions with the lowest (Eastern Serbia, n = 60) and of one of the regions with the highest Se serum levels reported in the past (Belgrade, n = 82), was determined by hydride generation atomic absorption spectrometry. Multivariate analysis was employed to determine the correlation between Se plasma levels and dietary intake data derived from food frequency questionnaires and laboratory tests. The mean plasma Se level of the participants was 84.3 ± 15.9 μg/L (range: 47.3–132.1 μg/L), while 46% of participants had plasma Se levels lower than 80 μg/L. Frequency of meat, egg, and fish consumption was significantly correlated with plasma selenium level (r = 0.437, p = 0.000). Selenium addition to animal feed in the quantity of 0.14 mg/kg contributed to the improvement of human plasma selenium levels by approximately 30 μg/L.
Mindfulness Is Associated with the Metabolic Syndrome among Individuals with a Depressive Symptomatology
Authors: Guyot Erika , Baudry Julia , Hercberg Serge , Galan Pilar , Kesse-Guyot Emmanuelle , Péneau Sandrine
Published: February 2018

The Metabolic Syndrome (MetS) is a major public health burden. Dispositional mindfulness has recently been associated with eating disorders, being overweight, and could therefore be associated with the MetS. We aimed to examine in a cross-sectional design the relationship between mindfulness, the MetS, and its risk factors in a large sample of the adult general population and the influence of depressive symptomatology on this association. Adults participating in the NutriNet-Santé study who had completed the Five Facets Mindfulness Questionnaire and attended a clinical and biological examination were available for inclusion. Multivariable logistic regression models adjusted for socio-demographic and lifestyle factors were performed. A total of 17,490 individuals were included. Among individuals with a depressive symptomatology, those with higher mindfulness were less likely to have a MetS (OR: 0.73, 95% CI: 0.57–0.93), a high waist circumference, a low HDL-cholesterol level and an elevated fasting blood glucose level (all p <0.05). In those without depressive symptomatology, individuals with higher mindfulness were less likely to have a high waist circumference (p <0.01). In conclusion, higher mindfulness was associated with lower odds of developing a MetS only among individuals with a depressive symptomatology.
Affinity Capillary Electrochromatography of Molecularly Imprinted Thin Layers Grafted onto Silica Capillaries Using a Surface-Bound Azo-Initiator and Living Polymerization
Authors: Giovannoli Cristina , Passini Cinzia , Nardo Fabio Di , Anfossi Laura , Baggiani Claudio , Nicholls Ian A.
Published: February 2018

Molecularly imprinted thin layers were prepared in silica capillaries by using two different surface polymerization strategies, the first using 4,4′-azobis(4-cyanovaleric acid) as a surface-coupled radical initiator, and the second, S-carboxypropyl-S’-benzyltrithiocarbonate as a reversible addition-fragmentation chain transfer (RAFT) agent in combination with 2,2′-azobisisobutyronitrile as a free radical initiator. The ability to generate imprinted thin layers was tested on two different polymerization systems: (i) a 4-vinylpyridine/ethylene dimethacrylate (4VP-EDMA) in methanol-water solution with 2,4,5-trichlorophenoxyacetic acid (2,4,5-T) as a template; and (ii) methacrylic acid/ethylene dimethacrylate (MAA-EDMA) in a chloroform solution with warfarin as the template molecule. The binding properties of the imprinted capillaries were studied and compared with those of the corresponding non-imprinted polymer coated capillaries by injecting the template molecule and by measuring its migration times relative to a neutral and non-retained marker. The role of running buffer hydrophobicity on recognition was investigated by studying the influence of varying buffer acetonitrile concentration. The 2,4,5-T-imprinted capillary showed molecular recognition based on a reversed phase mechanism, with a decrease of the template recognition in the presence of higher acetonitrile content; whereas warfarin-imprinted capillaries showed a bell-shaped trend upon varying the acetonitrile percentage, illustrating different mechanisms underlying imprinted polymer-ligand recognition. Importantly, the results demonstrated the validity of affinity capillary electrochromatography (CEC) to screen the binding properties of imprinted layers.
Impact of Nanoclays on the Biodegradation of Poly(Lactic Acid) Nanocomposites
Authors: Castro-Aguirre Edgar , Auras Rafael , Selke Susan , Rubino Maria , Marsh Terence
Published: February 2018

Poly(lactic acid) (PLA), a well-known biodegradable and compostable polymer, was used in this study as a model system to determine if the addition of nanoclays affects its biodegradation in simulated composting conditions and whether the nanoclays impact the microbial population in a compost environment. Three different nanoclays were studied due to their different surface characteristics but similar chemistry: organo-modified montmorillonite (OMMT), Halloysite nanotubes (HNT), and Laponite® RD (LRD). Additionally, the organo-modifier of MMT, methyl, tallow, bis-2-hydroxyethyl, quaternary ammonium (QAC), was studied. PLA and PLA bio-nanocomposite (BNC) films were produced, characterized, and used for biodegradation evaluation with an in-house built direct measurement respirometer (DMR) following the analysis of evolved CO2 approach. A biofilm formation essay and scanning electron microscopy were used to evaluate microbial attachment on the surface of PLA and BNCs. The results obtained from four different biodegradation tests with PLA and its BNCs showed a significantly higher mineralization of the films containing nanoclay in comparison to the pristine PLA during the first three to four weeks of testing, mainly attributed to the reduction in the PLA lag time. The effect of the nanoclays on the initial molecular weight during processing played a crucial role in the evolution of CO2. PLA-LRD5 had the greatest microbial attachment on the surface as confirmed by the biofilm test and the SEM micrographs, while PLA-QAC0.4 had the lowest biofilm formation that may be attributed to the inhibitory effect also found during the biodegradation test when the QAC was tested by itself.
Variational Destriping in Remote Sensing Imagery: Total Variation with L1 Fidelity
Authors: Yanovsky Igor , Dragomiretskiy Konstantin
Published: February 2018

This paper introduces a variational method for destriping data acquired by pushbroom-type satellite imaging systems. The model leverages sparsity in signals and is based on current research in sparse optimization and compressed sensing. It is based on the basic principles of regularization and data fidelity with certain constraints using modern methods in variational optimization, namely, total variation (TV),

L
1

fidelity, and the alternating direction method of multipliers (ADMM). The proposed algorithm, TV–

L
1

, uses sparsity-promoting energy functionals to achieve two important imaging effects. The TV term maintains boundary sharpness of the content in the underlying clean image, while the

L
1

fidelity allows for the equitable removal of stripes without over- or under-penalization, providing a more accurate model of presumably independent sensors with an unspecified and unrestricted bias distribution. A comparison is made between the TV–

L
2

model and the proposed TV–

L
1

model to exemplify the qualitative efficacy of an

L
1

striping penalty. The model makes use of novel minimization splittings and proximal mapping operators, successfully yielding more realistic destriped images in very few iterations.
Passive L-Band Microwave Remote Sensing of Organic Soil Surface Layers: A Tower-Based Experiment
Authors: Jonard François , Bircher Simone , Demontoux François , Weihermüller Lutz , Razafindratsima Stephen , Wigneron Jean-Pierre , Vereecken Harry
Published: February 2018

Organic soils play a key role in global warming because they store large amount of soil carbon which might be degraded with changing soil temperatures or soil water contents. There is thus a strong need to monitor these soils and, in particular, their hydrological characteristics using, for instance, space-borne L-band brightness temperature observations. However, there are still open issues with respect to soil moisture retrieval techniques over organic soils. In view of this, organic soil blocks with their vegetation cover were collected from a heathland in the Skjern River catchment in western Denmark and then transported to a remote sensing field laboratory in Germany where their structure was reconstituted. The controlled conditions at this field laboratory made it possible to perform tower-based L-band radiometer measurements of the soils over a period of two months. Brightness temperature data were inverted using a radiative transfer (RT) model for estimating the time variations in the soil dielectric permittivity and the vegetation optical depth. In addition, the effective vegetation scattering albedo parameter of the RT model was retrieved based on a two-step inversion approach. The remote estimations of the dielectric permittivity were compared to in situ measurements. The results indicated that the radiometer-derived dielectric permittivities were significantly correlated with the in situ measurements, but their values were systematically lower compared to the in situ ones. This could be explained by the difference between the operating frequency of the L-band radiometer (1.4 GHz) and that of the in situ sensors (70 MHz). The effective vegetation scattering albedo parameter was found to be polarization dependent. While the scattering effect within the vegetation could be neglected at horizontal polarization, it was found to be important at vertical polarization. The vegetation optical depth estimated values over time oscillated between 0.10 and 0.19 with a mean value of 0.13. This study provides further insights into the characterization of the L-band brightness temperature signatures of organic soil surface layers and, in particular, into the parametrization of the RT model for these specific soils. Therefore, the results of this study are expected to improve the performance of space-borne remote sensing soil moisture products over areas dominated by organic soils.
Combined Landsat and L-Band SAR Data Improves Land Cover Classification and Change Detection in Dynamic Tropical Landscapes
Authors: De Alban Jose , Connette Grant , Oswald Patrick , Webb Edward
Published: February 2018

Robust quantitative estimates of land use and land cover change are necessary to develop policy solutions and interventions aimed towards sustainable land management. Here, we evaluated the combination of Landsat and L-band Synthetic Aperture Radar (SAR) data to estimate land use/cover change in the dynamic tropical landscape of Tanintharyi, southern Myanmar. We classified Landsat and L-band SAR data, specifically Japan Earth Resources Satellite (JERS-1) and Advanced Land Observing Satellite-2 Phased Array L-band Synthetic Aperture Radar-2 (ALOS-2/PALSAR-2), using Random Forests classifier to map and quantify land use/cover change transitions between 1995 and 2015 in the Tanintharyi Region. We compared the classification accuracies of single versus combined sensor data, and assessed contributions of optical and radar layers to classification accuracy. Combined Landsat and L-band SAR data produced the best overall classification accuracies (92.96% to 93.83%), outperforming individual sensor data (91.20% to 91.93% for Landsat-only; 56.01% to 71.43% for SAR-only). Radar layers, particularly SAR-derived textures, were influential predictors for land cover classification, together with optical layers. Landscape change was extensive (16,490 km2; 39% of total area), as well as total forest conversion into agricultural plantations (3214 km2). Gross forest loss (5133 km2) in 1995 was largely from conversion to shrubs/orchards and tree (oil palm, rubber) plantations, and gross gains in oil palm (5471 km2) and rubber (4025 km2) plantations by 2015 were mainly from conversion of shrubs/orchards and forests. Analysis of combined Landsat and L-band SAR data provides an improved understanding of the associated drivers of agricultural plantation expansion and the dynamics of land use/cover change in tropical forest landscapes.
Quantification of Typhoon-Induced Phytoplankton Blooms Using Satellite Multi-Sensor Data
Authors: Pan Jiayi , Huang Lei , Devlin Adam T. , Lin Hui
Published: February 2018

Using satellite-based multi-sensor observations, this study investigates Chl-a blooms induced by typhoons in the Northwest Pacific (NWP) and the South China Sea (SCS), and quantifies the blooms via wind-induced mixing and Ekman pumping parameters, as well as pre-typhoon mixed-layer depth (MLD). In the NWP, the Chl-a bloom is more correlated with the Ekman pumping than with the other two parameters, with an R2 value of 0.56. In the SCS, the wind-induced mixing and Ekman pumping have comparable correlations with the Chl-a increase, showing R2 values of 0.4~0.6. However, the MLD exhibits a negative correlation with the Chl-a increase. A multi-parameter quantification model of the Chl-a bloom strength achieves better results than the single-parameter regressions, yielding a more significant R2 value of 0.80, and a lower regression rms of 0.18 mg·m−3 in the SCS, and the R2 value in the NWP is also improved compared with the single-parameter regressions. The multi-parameter quantification model of Chl-a blooms is more accurate in the SCS than in the NWP, due to the fact that nutrient profiles in the NWP are uniform from surface to a deep depth (300 m). Thus, the Chl-a blooms are more correlated with the upper ocean dynamical processes in the SCS where a shallower nutricline is found.
Using Micro-Molding and Stamping to Fabricate Conductive Polydimethylsiloxane-Based Flexible High-Sensitivity Strain Gauges
Authors: Han Chi-Jui , Chiang Hsuan-Ping , Cheng Yun-Chien
Published: February 2018

In this study, polydimethylsiloxane (PDMS) and conductive carbon nanoparticles were combined to fabricate a conductive elastomer PDMS (CPDMS). A high sensitive and flexible CPDMS strain sensor is fabricated by using stamping-process based micro patterning. Compared with conventional sensors, flexible strain sensors are more suitable for medical applications but are usually fabricated by photolithography, which suffers from a large number of steps and difficult mass production. Hence, we fabricated flexible strain sensors using a stamping-process with fewer processes than photolithography. The piezoresistive coefficient and sensitivity of the flexible strain sensor were improved by sensor pattern design and thickness change. Micro-patterning is used to fabricate various CPDMS microstructure patterns. The effect of gauge pattern was evaluated with ANSYS simulations. The piezoresistance of the strain gauges was measured and the gauge factor determined. Experimental results show that the piezoresistive coefficient of CPDMS is approximately linear. Gauge factor measurement results show that the gauge factor of a 140.0 μm thick strain gauge with five grids is the highest.
Online Aerial Terrain Mapping for Ground Robot Navigation
Authors: Peterson John , Chaudhry Haseeb , Abdelatty Karim , Bird John , Kochersberger Kevin
Published: February 2018

This work presents a collaborative unmanned aerial and ground vehicle system which utilizes the aerial vehicle’s overhead view to inform the ground vehicle’s path planning in real time. The aerial vehicle acquires imagery which is assembled into a orthomosaic and then classified. These terrain classes are used to estimate relative navigation costs for the ground vehicle so energy-efficient paths may be generated and then executed. The two vehicles are registered in a common coordinate frame using a real-time kinematic global positioning system (RTK GPS) and all image processing is performed onboard the unmanned aerial vehicle, which minimizes the data exchanged between the vehicles. This paper describes the architecture of the system and quantifies the registration errors between the vehicles.
Effect of Apium graveolens Extract Administration on the Pharmacokinetics of Captopril in the Plasma of Rats
Authors: Siska Siska , Mun`im Abdul , Bahtiar Anton , Suyatna Franciscus
Published: February 2018

Apium graveolens (celery) is an edible and traditionally medicinal plant that is used worldwide, among others for the treatment of hypertension. Combining celery with antihypertensive drugs can affect the pharmacodynamics and pharmacokinetics of the latter drugs. The aim of the study is to assess the effects of administrating the celery extract on captopril pharmacokinetics. Sprague-Dawley strain rats were divided into two groups (n = 6). Group I was given captopril (10 mg/kg Body Weight (BW)) orally, while Group II was pretreated with celery extract orally (40 mg/kg BW) an hour before administration of captopril. The blood samples were withdrawn at various intervals after drug administration. The captopril concentration was determined using liquid chromatography–mass spectrometry (LC-MS/MS) and from the blood data, the values of Ke, Cmax, Tmax, T1/2, and area under the curve (AUC) were calculated. The results showed that oral administration of the celery extract increased Cmax (38.67%), T1/2 (37.84%), and AUC (58.10%) and decreased Ke (27.45%) of captopril in Group II (celery + captopril) compared with Group I (captopril). In conclusion, celery extract can alter the pharmacokinetic of captopril when given in combination. The combination might be beneficial for the treatment of hypertension, as celery causes an increase in the plasma level of captopril, which can enhance its efficacy.
Maristem—Stem Cells of Marine/Aquatic Invertebrates: From Basic Research to Innovative Applications
Authors: Ballarin Loriano , Rinkevich Baruch , Bartscherer Kerstin , Burzynski Artur , Cambier Sebastien , Cammarata Matteo , Domart-Coulon Isabelle , Drobne Damjana , Encinas Juanma , Frank Uri , Geneviere Anne-Marie , Hobmayer Bert , Löhelaid Helike , Lyons Daniel , Martinez Pedro , Oliveri Paola , Peric Lorena , Piraino Stefano , Ramšak Andreja , Rakers Sebastian , Rentzsch Fabian , Rosner Amalia , Da Silva Tiago Henriques , Somorjai Ildiko , Suleiman Sherif , Coelho Ana Varela
Published: February 2018

The “stem cells” discipline represents one of the most dynamic areas in biomedicine. While adult marine/aquatic invertebrate stem cell (MISC) biology is of prime research and medical interest, studies on stem cells from organisms outside the classical vertebrate (e.g., human, mouse, and zebrafish) and invertebrate (e.g., Drosophila, Caenorhabditis) models have not been pursued vigorously. Marine/aquatic invertebrates constitute the largest biodiversity and the widest phylogenetic radiation on Earth, from morphologically simple organisms (e.g., sponges, cnidarians), to the more complex mollusks, crustaceans, echinoderms, and protochordates. These organisms contain a kaleidoscope of MISC-types that allow the production of a large number of novel bioactive-molecules, many of which are of significant potential interest for human health. MISCs further participate in aging and regeneration phenomena, including whole-body regeneration. For years, the European MISC-community has been highly fragmented and has established scarce ties with biomedical industries in an attempt to harness MISCs for human welfare. Thus, it is important to (i) consolidate the European community of researchers working on MISCs; (ii) promote and coordinate European research on MISC biology; (iii) stimulate young researchers to embark on research in MISC-biology; (iv) develop, validate, and share novel MISC tools and methodologies; (v) establish the MISC discipline as a forefront interest of biomedical disciplines, including nanobiomedicine; and (vi) establish collaborations with industries to exploit MISCs as sources of bioactive molecules. In order to fill the recognized gaps, the EC-COST Action 16203 “MARISTEM” has recently been launched. At its initial stage, the consortium unites 26 scientists from EC countries, Cooperating countries, and Near Neighbor Countries.
China’s Confucius Institute in Indonesia: Mobility, Frictions and Local Surprises
Authors: Theo Rika , Leung Maggi W.H.
Published: February 2018

China’s cross-border language promotion body, the Confucius Institute (CI), has proliferated along with the mobility of Chinese capital and people worldwide. It embodies the ‘Going Out’ state strategy that promotes the global spread of Chinese capital, ideas, culture and people. Often seen as a vehicle of China’s power and influence, the CI has attracted much suspicion and even rejection as compared to similar institutions of other states. This paper examines the mobility of the CI and the encountered frictions when it lands in particular places, problematizing the commonly assumed unidirectional impact of the cross-border institution as a mighty soft power instrument. Specifically, it analyses the frictions of the CI’s establishment in Indonesia, where racial and political narratives on China and Chinese-Indonesians have long prevailed. Three cases are presented: one at the national level in Jakarta and two at the local level in the cities of Bandung and Makassar. By elaborating how frictions are created, resisted and managed differently, this paper illustrates the interplay of actors and power relations in the mobility of the CI, which in turn gives rise to particular local surprises. This paper also underlines the role of the Chinese-Indonesian diaspora as important bridge-builders of their two homelands.
Quinoa Expansion in Peru and Its Implications for Land Use Management
Authors: Bedoya-Perales Noelia S. , Pumi Guilherme , Mujica Angel , Talamini Edson , Domingos Padula Antonio
Published: February 2018

Quinoa (Chenopodium quinoa Willd.) has gained prominence worldwide over recent years and suddenly Peru has emerged as a major player in the global quinoa market. This study aims to analyze the expansion of quinoa farming in Peru in the period 1995–2014 and to discuss the changes in land-use the country has experienced as a result of the boom in the global demand for quinoa. Two statistical approaches, principal component analysis (PCA) and exponential smoothing, were applied in the data analysis to explore the evolution of the quinoa boom in Peru by periods and to forecast what the acreage expansion rate would have been if the boom had not occurred. The results show that the quinoa boom was responsible for an increase of 43% in the number of hectares planted with quinoa in 2014, in relation to the number predicted if there had been no boom. This provoked an acceleration of production in traditional quinoa farming areas and the extension of this activity to new regions. The consequences are already apparent in the land-use changes seen in Peru, namely the: (i) displacement; (ii) rebound; and (iii) cascade effects.