bluuebunny/crossref_metadata_2025 huggingface dataset:
Improving the clinical performance of blood-based DNA methylation biomarkers utilizing locus-specific epigenetic heterogeneity
Authors: Miller Brendan F. , Pisanic Thomas R. , Margolin Gennady , Petrykowska Hanna M. , Athamanolap Pornpat , Goncearenco Alexander , Osei-Tutu Akosua , Annunziata Christina M. , Wang Tza-Huei , Elnitski Laura
Published: March 2019

Background

Variation in intercellular methylation patterns can complicate the use of methylation biomarkers for clinical diagnostic applications such as blood-based cancer testing. Here, we describe development and validation of a methylation density binary classification method called EpiClass (available for download at https://github.com/Elnitskilab/EpiClass), that can be used to predict and optimize the performance of methylation biomarkers, particularly in challenging, heterogeneous samples such as liquid biopsies. This approach is based upon leveraging statistical differences in single-molecule sample methylation density distributions to identify ideal thresholds for sample classification.

Results

We developed and tested the classifier using reduced representation bisulfite sequencing (RRBS) data derived from ovarian carcinoma tissue DNA and controls. We used these data to perform in silico simulations using methylation density profiles from individual epiallelic copies of ZNF154, a genomic locus known to be recurrently methylated in numerous cancer types. From these profiles, we predicted the performance of the classifier in liquid biopsies for the detection of epithelial ovarian carcinomas (EOC). In silico analysis indicated that EpiClass could be leveraged to better identify cancer-positive liquid biopsy samples by implementing precise thresholds with respect to methylation density profiles derived from circulating cell-free DNA (cfDNA) analysis. These predictions were confirmed experimentally using DREAMing to perform digital methylation density analysis on a cohort of low volume (1-mL) plasma samples obtained from 26 EOC-positive and 41 cancer-free women. EpiClass performance was then validated in an independent cohort of 24 plasma specimens, derived from a longitudinal study of 8 EOC-positive women, and 12 plasma specimens derived from 12 healthy women, respectively, attaining a sensitivity/specificity of 91.7%/100.0%. Direct comparison of CA-125 measurements with EpiClass demonstrated that EpiClass was able to better identify EOC-positive women than standard CA-125 assessment. Finally, we used independent whole genome bisulfite sequencing (WGBS) datasets to demonstrate that EpiClass can also identify other cancer types as well or better than alternative methylation-based classifiers.

Conclusions

Our results indicate that assessment of intramolecular methylation density distributions calculated from cfDNA facilitate the use of methylation biomarkers for diagnostic applications. Furthermore, we demonstrated that EpiClass analysis of ZNF154 methylation was able to outperform CA-125 in the detection of etiologically-diverse ovarian carcinomas, indicating the broad utility of ZNF154 for use as a biomarker of ovarian cancer.
A haplotype-aware de novo assembly of related individuals using pedigree graph
Authors: Garg Shilpa , Aach John , Li Heng , Durbin Richard , Church George
Published: March 2019

Motivation

Reconstructing high-quality haplotype-resolved assemblies for related individuals of various species has important applications in understanding Mendelian diseases along with evolutionary and comparative genomics. Through major genomics sequencing efforts such as the Personal Genome Project, the Vertebrate Genome Project (VGP), the Earth Biogenome Project (EBP) and the Genome in a Bottle project (GIAB), a variety of sequencing datasets from mother-father-child trios of various diploid species are becoming available.

Current trio assembly approaches are not designed to incorporate long-read sequencing data from parents in a trio, and therefore require relatively high coverages of costly long-read data to produce high-quality assemblies. Thus, building a trio-aware assembler capable of producing accurate and chromosomal-scale diploid genomes in a pedigree, while being cost-effective in terms of sequencing costs, is a pressing need of the genomics community.

Results

We present a novel pedigree-graph-based approach to diploid assembly using accurate Illumina data and long-read Pacific Biosciences (PacBio) data from all related individuals, thereby generalizing our previous work on single individuals. We demonstrate the effectiveness of our pedigree approach on a simulated trio of pseudo-diploid yeast genomes with different heterozygosity rates, and real data from Arabidopsis Thaliana. We show that we require as little as 30× coverage Illumina data and 15× PacBio data from each individual in a trio to generate chromosomal-scale phased assemblies. Additionally, we show that we can detect and phase variants from generated phased assemblies.

Availability

https://github.com/shilpagarg/WHdenovo

Contact

shilpa_garg@hms.harvard.edu, gchurch@genetics.med.harvard.edu
Loss of Bardet-Biedl syndrome proteins causes synaptic aberrations in principal neurons
Authors: Haq Naila , Schmidt-Hieber Christoph , Sialana Fernando J. , Ciani Lorenza , Heller Janosch P. , Stewart Michelle , Bentley Liz , Wells Sara , Rodenburg Richard J. , Nolan Patrick M , Forsythe Elizabeth , Wu Michael C. , Lubec Gert , Häusser Michael , Beales Philip L. , Christou-Savina Sofia
Published: March 2019

Bardet-Biedl syndrome (BBS), a ciliopathy, is a rare genetic condition characterised by retina degeneration, obesity, kidney failure and cognitive impairment. In spite of a progress made in general understanding of BBS aetiology, the molecular mechanism of cognitive impairment remains elusive. Here we report that loss of Bardet-Biedl syndrome proteins causes synaptic dysfunction in principal neurons providing possible explanation for cognitive impairment phenotype in BBS patients. Using synaptosomal proteomics and immunocytochemistry we demonstrate the presence of Bbs in postsynaptic density of hippocampal neurons. Loss of Bbs results in the significant reduction of dendritic spines in principal neurons ofBbsmice models. Furthermore, we demonstrate that spine deficiency correlates with events that destabilize spine architecture, such as, impaired spine membrane receptors signalling known to be involved in the maintenance of dendritic spines. Finally, we show that voluntary exercise rescues spine deficiency in the neurons. Based on our data, we propose a model in which Bbs proteins, similar to their function in primary cilia, regulate trafficking of signalling receptors into and out of the membrane of dendritic spines, thus providing the basis for synaptic plasticity.
Generation of scale-invariant sequential activity in linear recurrent networks
Authors: Liu Yue , Howard Marc W.
Published: March 2019

Sequential neural activity has been observed in many parts of the brain and has been proposed as a neural mechanism for memory. The natural world expresses temporal relationships at a wide range of scales. Because we cannot know the relevant scales a priori it is desirable that memory, and thus the generated sequences, are scale-invariant. Although recurrent neural network models have been proposed as a mechanism for generating sequences, the requirements for scale-invariant sequences are not known. This paper reports the constraints that enable a linear recurrent neural network model to generate scale-invariant sequential activity. A straightforward eigendecomposition analysis results in two independent conditions that are required for scaleinvariance for connectivity matrices with real, distinct eigenvalues. First, the eigenvalues of the network must be geometrically spaced. Second, the eigenvectors must be related to one another via translation. These constraints are easily generalizable for matrices that have complex and distinct eigenvalues. Analogous albeit less compact constraints hold for matrices with degenerate eigenvalues. These constraints, along with considerations on initial conditions, provide a general recipe to build linear recurrent neural networks that support scale-invariant sequential activity.
Hi-C guided assemblies reveal conserved regulatory topologies on X and autosomes despite extensive genome shuffling
Authors: Renschler Gina , Richard Gautier , Valsecchi Claudia Isabelle Keller , Toscano Sarah , Arrigoni Laura , Ramirez Fidel , Akhtar Asifa
Published: March 2019

Genome rearrangements that occur during evolution impose major challenges on regulatory mechanisms that rely on three-dimensional genome architecture. Here, we developed a scaffolding algorithm and generated chromosome-length assemblies from Hi-C data for studying genome topology in three distantly relatedDrosophilaspecies. We observe extensive genome shuffling between these species with one synteny breakpoint after approximately every six genes. A/B compartments, a set of large gene-dense topologically associating domains (TADs) and spatial contacts between high-affinity sites (HAS) located on the X chromosome are maintained over 40 million years, indicating architectural conservation at various hierarchies. Evolutionary conserved genes cluster in the vicinity of HAS, while HAS locations appear evolutionarily flexible, thus uncoupling functional requirement of dosage compensation from individual positions on the linear X chromosome. Therefore, 3D architecture is preserved even in scenarios of thousands of rearrangements highlighting its relevance for essential processes such as dosage compensation of the X chromosome.
Rational nanotoolbox with theranostic potential for medicated pro-regenerative corneal implants
Authors: Patra Hirak K. , Azharuddin Mohammad , Islam Mohammad M. , Papapavlou Georgia , Deb Suryyani , Zhu Geyunjian Harry , Romu Thobias , Dhara Ashis K. , Jafari Mohammad J. , Gadheri Amineh , Hinkula Jorma , Rajan Madhavan S , Slater Nigel
Published: March 2019

Cornea diseases are a leading cause of blindness and the disease burden is exacerbated by the increasing shortage around the world for cadaveric donor corneas. Despite the advances in the field of regenerative medicine, successful transplantation of laboratory made artificial corneas has not been fully realised in clinical practice. The causes of failure of such artificial corneal implants are multifactorial and include latent infections from viruses and other micorbes, enzyme over-expression, implant degradation, extrusion or delayed epithelial regeneration. Therefore, there is an urgent unmet need for developing customized corneal implants to suit the host environment, counter the effects of inflammation or infection and that are able to track early signs of implant failurein situ. In the present work, we describe a nano toolbox comprising tools for drug release and in addition capable of being infection responsive, promoting regeneration including non-invasive monitoring ofin situcorneal environment. These nano constructs can be incorporated within pro-regenerative biosynthetic implants, transforming them into theranostic devices able to respond to biological changes following implantation.
Microtubules Regulate Spatial Localization and Availability of Insulin Granules in Pancreatic Beta Cells
Authors: Bracey Kai M. , Ho Kung-Hsien , Yampolsky Dmitry , Gu Guoqiang , Kaverina Irina , Holmes William R.
Published: March 2019

Two key prerequisites for glucose stimulated insulin secretion (GSIS) in Beta cells are the proximity of insulin granules to the plasma membrane and their anchoring or docking to the plasma membrane (PM). While recent evidence has indicated that both of these factors are altered in the context of diabetes, it is unclear what regulates localization of insulin and its interactions with the PM within single cells. Here we demonstrate that microtubule (MT) motor mediated transport dynamics have a critical role in regulating both factors. Super-resolution imaging shows that while the MT cytoskeleton resembles a random meshwork in the cells’ interior, MTs near the cells surface are preferentially aligned with the PM. Computational modeling demonstrates two consequences of this alignment. First, this structured MT network preferentially withdraws granules from the PM. Second, the binding and transport of insulin granules by MT motors prevents their stable anchoring to the PM. The MT cytoskeleton thus negatively regulates GSIS by both limiting the amount of insulin proximal to the PM and preventing/breaking interactions between the PM and the remaining nearby insulin. These results predict that altering MT structure in beta cells can be used to tune GSIS. Thus, our study points to a potential of an alternative therapeutic strategy for diabetes by targeting specific MT regulators.
Estimating narrow-sense heritability from genome-wide data in admixed populations
Authors: Athanasiadis Georgios , Speed Doug , Andersen Mette K. , Appel Emil V. R. , Grarup Niels , Brandslund Ivan , Jørgensen Marit Eika , Lytken Larsen Christina Viskum , Bjerregaard Peter , Hansen Torben , Albrechtsen Anders
Published: March 2019

Finding an efficient framework for estimating total narrow-sense heritability in admixed populations remains an open question. In this work, we used extensive simulations to evaluate existing linear mixed model frameworks in estimating total narrow-sense heritability in two population-based cohorts from Greenland and compared the results to data from unadmixed individuals from Denmark. When our analysis focused on Greenlandic sib pairs, the model with two relationship matrices, one capturing identity by descent and one capturing identity by state, returned heritability estimates close to the true simulated value, while using each of the two matrices alone led to downward biases. When phenotypes correlated with ancestry, heritability estimates were inflated. Based on these observations, we propose a post-estimation PCA-based adjustment that recovers successfully the true simulated heritability. We use this knowledge to estimate the heritability of ten quantitative traits from the two Greenlandic cohorts and report differences such as lower heritability for height in Greenlanders compared to Europeans. In conclusion, narrow-sense heritability in admixed populations is best estimated using a mixture of genetic relationship matrices on individuals with at least one first-degree relative included in the sample.
PERK inhibition mitigates restenosis and thrombosis - a potential low-thrombogenic anti-restenotic paradigm
Authors: Wang Bowen , Zhang Mengxue , Urabe Go , Chen Guojun , Wheeler Debra , Dornbos David , Huttinger Allyson , Huang Yitao , Nimjee Shahid , Gong Shaoqin , Guo Lian-Wang , Kent K Craig
Published: March 2019

Background

Drug-eluting stents (DES) represent the main-stream management of restenosis following treatments of occlusive cardiovascular diseases. However, DES cannot eliminate instent restenosis yet exacerbate thrombogenic risks. To achieve dual inhibition of restenotic smooth muscle cell (SMC) de-differentiation/proliferation and thrombogenic endothelial cell (EC) dysfunction, a common target in both cell types, has been long-sought after. We evaluated the potential of protein kinase RNA-like endoplasmic reticulum kinase (PERK) as such a target for low-thrombogenic anti-restenotic intervention.

Methods and Results

We used a rat angioplasty model of restenosis and a FeCl3-induced mouse model of thrombosis. Loss-or gain-of-function was achieved by PERK inhibition (GSK2606414, siRNA) or overexpression (adenovirus). Restenosis was robustly mitigated by GSK2606414 administered either via injected (i.v.) lesion-homing platelet membrane-coated nanoclusters or a perivascular hydrogel; it was enhanced by PERK transgene. Whereas PERK inhibition blocked, its overexpression exacerbated PDGF-induced human aortic SMC de-differentiation (reduced smooth muscle α-actin or αSMA) and proliferation (BrdU incorporation). Further, PERK activity promoted STAT3 activation but inhibited SRF transcriptional (luciferase) activity; its protein co-immunoprecipitated with STAT3 and also MRTF-A, the SRF activator for αSMA transcription. Importantly, PERK inhibition also prevented TNFα-induced impairment of human EC growth and upregulation of thrombogenic tissue factor, both in vitro and ex vivo. In vivo, oral gavage of GSK2606414 preserved ~50% of the normal blood flow 60 min after FeCl3-induced vascular injury.

Conclusions

PERK inhibition is dual beneficial in mitigating restenosis and thrombosis, thus implicating a potential design for anti-restenotic intervention to overcome the thrombogenicity of DES.
Naturally Produced Lovastatin Modifies the Histology and Proteome Profile of Goat Skeletal Muscle
Authors: Leo Teik Kee , Garba Sani , Abubakar Danmaigoro , Sazili Awis Qurni , Candyrine Su Chui Len , Jahromi Mohammad Faseleh , Goh Yong Meng , Ronimus Ron , Muetzel Stefan , Liang Juan Boo
Published: March 2019

Enteric methane formation in ruminants is one of the major contributors to climate change. We have reported that supplementation of naturally produced lovastatin reduced methane emissions in goats without adversely affecting rumen fermentation and animal performance, except that at higher level, lovastatin can have a negative effect on the palatability of the formulated diet. As statins are associated with the development of muscle-related adverse effects at higher than recommended therapeutic doses, this study was conducted to examine the effects of lovastatin on the histology and proteome profile of goat skeletal muscle. A total of 20 intact male Saanen goats were randomly assigned in equal numbers to 4 groups, and fed with a total mixed ration containing 50% rice straw, 22.8% concentrates and 27.2% of various proportions of untreated or treated palm kernel cake (PKC) to achieve the target daily intake levels of 0 (Control), 2 (Low), 4 (Medium) or 6 (High) mg lovastatin/kg body weight (BW). Histological examination discovered that the longissimus thoracis et lumborum muscle of animals from the Medium and High treatment groups showed abnormalities in terms of necrosis, degeneration, interstitial space and vacuolation. Western blot analysis conducted on the myosin heavy chain showed that the immunoreactivity of myosin heavy chain in the High treatment group was significantly lower than the Control, Low and Medium treatment groups. Comparisons between control and lovastatin-treated groups demonstrated that lovastatin supplementation induced complex modifications to the protein expression patterns of the longissimus thoracis et lumborum muscle of the goat. There were 30, 26 and 24 proteins differentially expressed in Low, Medium and High treatment groups respectively, when compared to the Control group. Supplementation of lovastatin down-regulated proteins involved in carbohydrate and creatine metabolism, indicative of reduced energy production, and may have contributed to the skeletal muscle damage. Supplementation of naturally produced lovastatin induced muscle damage in longissimus thoracis et lumborum muscle of goats with increasing dosages, particularly at 6mg/kg BW. In addition, proteomics analysis revealed that lovastatin supplementation induced complex modifications to the protein expressions of skeletal muscle of goats which may have contributed to the observed skeletal muscle damage. Present study suggested that supplementation of naturally-produced lovastatin at 6mg/kg BW could adversely affecting health and wellbeing of the animals.
Sex differences in reward- and punishment-guided actions
Authors: Chowdhury Tara G. , Wallin-Miller Kathryn G. , Rear Alice A. , Park Junchol , Diaz Vanessa , Simon Nicholas W. , Moghaddam Bita
Published: March 2019

Differences in the prevalence and presentation of psychiatric illnesses in men and women suggest that neurobiological sex differences confer vulnerability or resilience in these disorders. Rodent behavioral models are critical for understanding the mechanisms of these differences. Reward processing and punishment avoidance are fundamental dimensions of the symptoms of psychiatric disorders. Here we explored sex differences along these dimensions using multiple and distinct behavioral paradigms. We found no sex difference in reward-guided associative learning but a faster punishment-avoidance learning in females. After learning, females were more sensitive than males to probabilistic punishment but less sensitive when punishment could be avoided with certainty. No sex differences were found in reward-guided cognitive flexibility. Thus, sex differences in goal-directed behaviors emerged selectively when there was an aversive context. These differences were critically sensitive to whether the punishment was certain or unpredictable. Our findings with these new paradigms provide conceptual and practical tools for investigating brain mechanisms that account for sex differences in susceptibility to anxiety and impulsivity. They may also provide insight for understanding the evolution of sex-specific optimal behavioral strategies in dynamic environments.
A quantitative modular modeling approach reveals the consequences of different A20 feedback implementations for the NF-kB signaling dynamics
Authors: Mothes Janina , Ipenberg Inbal , Arslan Seda Çöl , Benary Uwe , Scheidereit Claus , Wolf Jana
Published: March 2019

Signaling pathways involve complex molecular interactions and are controlled by non-linear regulatory mechanisms. If details of regulatory mechanisms are not fully elucidated, they can be implemented by different, equally reasonable mathematical representations in computational models. The study presented here focusses on NF-κB signaling, which is regulated by negative feedbacks via IκBα and A20. A20 inhibits NF-κB activation indirectly through interference with proteins that transduce the signal from the TNF receptor complex to activate the IκB kinase (IKK) complex. We focus on the question how different implementations of the A20 feedback impact the dynamics of NF-κB. To this end, we develop a modular modeling approach that allows combining previously published A20 modules with a common pathway core module. The resulting models are based on a comprehensive experimental data set and therefore show quantitatively comparable NF-κB dynamics. Based on defined measures for the initial and long-term behavior we analyze the effects of a wide range of changes in the A20 feedback strength, the IκBα feedback strength and the TNFα stimulation strength on NF-κB dynamics. This shows similarities between the models but also model-specific differences. In particular, the A20 feedback strength and the TNFα stimulation strength affect initial and long-term NF-κB concentrations differently in the analyzed models. We validated our model predictions experimentally by varying TNFα concentrations applied to HeLa cells. These time course data indicate that only one of the A20 feedback models appropriately describes the impact of A20 on the NF-κB dynamics.

Author summary

Models are abstractions of reality and simplify a complex biological process to its essential components and regulations while preserving its particular spatial-temporal characteristics. Modelling of biological processes is based on assumptions, in part to implement the necessary simplifications but also to cope with missing knowledge and experimental information. In consequence, biological processes have been implemented by different, equally reasonable mathematical representations in computational models. We here focus on the NF-κB signaling pathway and develop a modular modeling approach to investigate how different implementations of a negative feedback regulation impact the dynamical behavior of a computational model. Our analysis shows similarities of the models with different implementations but also reveals implementation-specific differences. The identified differences are used to design and perform informative experiments that elucidate unknown details of the regulatory feedback mechanism.
Attract customers with a cold look: when do we prefer cold representative
Authors: Xie Zhipeng , Zhao Jing , Wang Tao
Published: March 2019

Purpose

The purpose of this paper is to examine the contradictory effect of coldness in advertisement and brand logo design.

Design/methodology/approach

The authors conduct four experiments to test the influences of coldness on consumers’ attitude. In the first two experiments, the researchers use real/virtual brand names to test the moderating effect of brand status and customer power; in Study 3, the researchers test the moderating effect of autonomy; while in the last experiment, the findings in the previous experiments are extended to explain similar effect of cold/warm brand logo designs.

Findings

This research finds that coldness also brings benefit to brands under certain circumstances. More specifically, cold endorsers/brand logo designs are only beneficial for brands of high (vs low) status, and can only attract consumers who experience high power (vs low power). Such effects are mediated by consumers’ perceived brand autonomy.

Research limitations/implications

This research urges the managers to analyze the characteristics of the brand and its target consumer. It also points out that the effectiveness of warm/cold representative derives from the customer perceived brand autonomy.

Originality/value

The authors’ contributions to the literature are as follows. First, this research examines the relationship between coldness and autonomy, which enables us to expand the findings to various contexts; second, this research expands the horizon of autonomy theory by identifying customer power and brand status as its moderators.
Pursuing superior performance of service innovation through improved corporate social responsibility
Authors: Li Lan , Li Gang , Yang Xue , Yang Zhilin
Published: March 2019

Purpose

The purpose of this paper is to investigate the impact of corporate social responsibility (CSR) on the performance of service innovation (PSI), and the mediating effect of knowledge acquisition.

Design/methodology/approach

Drawing on social exchange and knowledge management theories, this paper establishes a relevant conceptual model and adopts a hierarchical regression analysis to examine the model with a data set of 298 firms from China.

Findings

CSR positively affects the PSI; however, the effects vary when firms take responsibility for different stakeholders. CSR for the same group of stakeholders influences differently the short-term financial and long-term non-financial PSI, whereas knowledge acquisition mediates the impact of employee and customer CSR on PSI, but not the impact of community CSR on PSI.

Practical implications

Managers could improve the PSI of the firm by strategically assuming CSR and by managing corporate knowledge acquisition activities.

Originality/value

This study contributes to the service innovation literature by identifying the influence of particular types of CSR on PSI, and by highlighting the influencing mechanism of knowledge acquisition. It extends scholarly understanding of the antecedents of PSI as well as the business returns to CSR.
Celebrity poses and consumer attitudes in endorsement advertisements
Authors: Liu Yongdan , Liu Matthew Tingchi
Published: March 2019

Purpose

The purpose of this paper is to investigate the effects of celebrity’s poses on consumer attitudes towards the endorsement advertisement by drawing from perceptual fluency hypotheses.

Design/methodology/approach

Study 1 used a single-factor, two-condition (distinctive pose and casual pose) between-subject design. Both Study 2a and Study 2b employed a single-factor, two-condition (distinctive pose, casual pose) between-subject design and tested the mediator of pose matchiness. Study 3 employed a 2 (pose condition: distinctive, casual)×2 (cognitive capacity: no load, load) between-subject design to test the moderator. All data were sourced from more than 600 respondents in China.

Findings

Study 1 illustrated that the existence of a distinctive pose can lead to higher consumer attitudes regarding advertising stimuli and the endorsed brands as well as more positive behavioural intentions towards endorsed products. Study 2a and Study 2b replicated such finding and demonstrated that the feeling of pose matchiness mediates the relationship between celebrities’ pose and endorsement outcomes. Study 3 further revealed that the cognitive capacity moderates such a relationship, that is, that the effect of a distinctive pose is stronger (lesser) when audiences’ cognitive capacity is loaded (not loaded).

Originality/value

Research efforts to date examining the nature of celebrity advertisement have been limited to celebrity’s faces and facial expressions. Little investigation in the marketing domain has considered the consequences of celebrities’ poses. This study takes the first step in revealing the positive effect of distinctive celebrity poses in product endorsement.
The application of multi-attribute utility theory for a market share-based design evaluation
Authors: BuHamdan Samer , Alwisy Aladdin , Bouferguene Ahmed , Al-Hussein Mohamed
Published: March 2019

Purpose

The purpose of this paper is to use the concepts of the multi-attribute utility theory to develop a model to evaluate the design of low-density residential units to increase the profit of the company from a certain design, by assessing the changes in the market shares as a result of the built unit’s attributes.

Design/methodology/approach

The proposed platform consists of two stages: Stage I or relational model development and Stage II or design evaluation. Stage I is concerned with developing a mathematical model that links design variables (e.g. the R-Value of the building envelope and construction material) with the assessment attributes (e.g. price and carbon emissions). Stage II ensures the fulfillment of the corporation’s goals in maximizing profit and market shares using multi-attribute utility theory.

Findings

The application of the proposed model on a case study – a single-family house – shows that reducing the selling price of the unit is not always the best marketing strategy builders should pursue to increase their sales and accordingly their profit, as accounting for other attributes (e.g. performance, operational cost and environmental impact) leads to larger changes in the market shares and accordingly in profit.

Research limitations/implications

The limitations of this research are manifested in the following points: it does not account for the impact of the marketing campaigns on the market shares; it considers the profit as a percentage of the construction cost; and it has not been validated on high-density residential buildings.

Practical implications

This research provides speculative builders with a platform that allows the objective evaluation of houses’ designs prior to introducing them to the market so builders can increase their market shares and consequently their profit. The proposed platform also contributes to increasing the sustainable performance of the housing industry, as it allows for the assessment of the design against economic, environmental and social attributes concurrently, which ensures a balanced consideration of the built houses on sustainability pillars.

Social implications

The proposed platform for design evaluation extends the assessment attributes beyond the traditionally considered economic and environmental attributes. By doing so, it assists decision-makers in evaluating the potential social influence of the proposed design and, as a result, reduces the unwanted impact.

Originality/value

This research combines the concepts of multi-attribute utility with market studies to develop an objective decision support tool for evaluating the design of speculative houses to increase the sustainable performance of the builders without compromising on their profit.
Managers’ views on how intellectual capital is recognized and managed in practice
Authors: Tyskbo Daniel
Published: March 2019

Purpose

The purpose of this paper is to advance the understanding of how intellectual capital (IC) unfolds in practice in organizations. This is done by answering the research question of how IC is recognized and managed in practice as expressed by managers.

Design/methodology/approach

An explorative, empirical and multiple case study was conducted, investigating four Swedish firms.

Findings

This paper illustrates how IC was recognized and managed in practice despite managers expressing uncertainty of what the IC concept means. More or less direct, formalized and purposeful ways were adopted. The IC elements and practices most important from a management perspective were those aligned with the overall strategy, but were seldom what was visible in financial reports.

Research limitations/implications

The use of an explorative, multiple case study limits the generalizability. However, the rich view gained of how IC unfolds in practice may not always be possible using large sample, survey-studies. Future research is therefore suggested to take this paper’s insights further and investigate IC in other organizations and in other national contexts.

Originality/value

This paper responds to the calls for third stage IC research, by showing how IC management in practice may not be as clear and straightforward as researchers tend to assume. It also adds to the importance debate on IC accountingization, by reflecting on how an accounting dominance may not fully capture IC inside organizations. A number of practical contributions are also made.
Delta model application for developing customer lifetime value
Authors: Hajipour Bahman , Esfahani Molud
Published: March 2019

Purpose

The purpose of this paper is to evaluate the relationship between strategy and customer lifetime value (CLV). A new model was proposed for defining customers’ values based on the RFM model and segmenting bank customers using the K-means algorithm. In addition, the authors combined a new category with the delta model in order to analyze the behavior of each cluster.

Design/methodology/approach

This case study was based on an applied method following its objectives and a descriptive-analytic method in terms of data collection. In this research, the AHP, data mining and K-means clustering methods, as well as the discriminant analysis were applied for computing the weights of the indices, examining the relationship between the identified variables, clustering the records and ensuring the clustering accuracy based on the RFM model, respectively.

Findings

The paper confirmed the relationship between the strategies and CLV. For a cluster whose strategy was the best product, customers had a minimal CLV. For a cluster whose strategy was based on total customer solutions, customers had a median CLV. For a cluster whose strategy was a lock-in system, customers had a maximal CLV. The results suggested that the delta model with these three strategies could act as the CLV developers in two stages: conversion of transient customers to attached customers and conversion of attached customers to locked-in customers.

Research limitations/implications

One of the limitations of this study was the lack of access to all the bank accounts and assessment of only the strategy type, while highlighting the exact association between every component of the strategies (e.g. structure, environment, etc.) and CLV as a dependent variable deemed to be of a great necessity. Hence, it is recommended that several studies on the relationships presented in this paper be performed to provide further insights into and guidelines on this issue in the future.

Practical implications

This study emphasized the relevance of strategy and CLV. Managers should differently treat customers in distinct CLV and loyalty levels. In other words, managers must segment their customers based on CLV and apply appropriate strategies for each segment.

Originality/value

This research tried to fulfill an identified need to study how strategy can be effect CLV through the application of the delta model with three strategic options.
Proximate, mineral composition and sensory evaluation of coffee and kola flavoured biscuits
Authors: Gyedu-Akoto Esther , Opoku Stephen Yaw , Ofosu-Agyei Emmanuel
Published: March 2019

Purpose

Refined wheat flour is a key ingredient for preparing biscuits because of its gluten content. However, biscuits prepared from refined flour are characterised with low proteins, fat and mineral content. This study aims to improve the nutritive and sensory values of biscuits using roasted coffee and dry kola powders.

Design/methodology

The powders were incorporated into biscuits at different levels of 5, 10, 15 and 20 per cent. Proximate and sensory analyses were conducted on the products obtained to determine the effects of the powders on the products.

Findings

The coffee biscuits had carbohydrate, protein, fat and energy contents of 57.0-69.5 per cent, 3.3-5.5 per cent, 20.4-31.8 per cent and 389-404 kcal/g, respectively, while those of the kola biscuits were 67.0-70.5 per cent, 1.5-3.5 per cent, 20.4-23.8 per cent and 384-395 kcal/g, respectively. Sensory evaluation of both products showed that coffee significantly improved sensory attributes such as flavour (p = 0.03) and colour (p = 0.00). Thus coffee powder can be used to enhance both the nutritional and sensory qualities of biscuits.

Originality/value

This study was to promote the use of coffee and kola in locally produced baked products and meet consumer demand for healthy and natural foods.
Assessing the impact of the new auditor’s report
Authors: Li Hong , Hay David , Lau David
Published: March 2019

Purpose

Changes to the auditor’s report have been proposed and issued internationally to provide more relevant information to users and enhance the perceived value of financial statement audits. This paper aims to investigate the impact of audit reporting changes on audit quality and audit fees in the New Zealand context.

Design/methodology/approach

The authors examined audit quality measured by absolute abnormal accruals and audit fees for New Zealand listed companies.

Findings

The evidence suggests the enhanced audit reports were followed by an improvement in audit quality as proxied by a reduction in absolute abnormal accruals upon the adoption of the new audit reporting requirements. There was also a significant increase in audit fees.

Practical implications

Although the new auditor reporting requirements are associated with improvements in audit quality, such benefit does not come without cost.

Originality/value

The study provides evidence about the impact of this recent substantial reform to auditing.
Does pension plan reporting readability affect earnings volatility?
Authors: Lin Ruey-Ching , Chen Tsung-Kang , Tseng Yi-Jie , Chang Chih-Kai
Published: March 2019

Purpose

The purpose of this study is to explore whether pension plan reporting readability affects earnings volatility. Moreover, as SFAS 158 requires firms to fully recognize their funded status on the balance sheet, the firms’ pension liabilities (inside debts) and financing ability have the corresponding change. This study further investigates whether pension plan reporting readability affects earnings volatility from the SFAS 158 and funded status perspectives.

Design/methodology/approach

This study follows Li (2008), Lehavy et al. (2011) and Rennekamp (2012) to use the FOG and SMOG variables as the readability proxies and investigates whether pension plan reporting readability affects earnings volatility from the perspectives of inside debts and SFAS 158 by using a sample of 3,077 American firms from the year 2006 to 2009.

Findings

Empirical results of this study show that firms with low readability of pension plan reporting have high earnings volatility, revealing that less readable pension plan reporting increases the assessed variance of a firm’s inside debts, financing flexibility, investment ability and therefore profitability. In addition, the implement of SFAS 158 enhances the effect of pension plan reporting readability on earnings volatility. Moreover, the authors also find that the funded status plays a moderating role for the effect of pension plan reporting readability on earnings volatility. Finally, the results are robust to endogeneity issue.

Research limitations/implications

Earnings stability measures how consistently earnings have been generated over time, and its importance has been acknowledged by most firms. For example, prior literature has documented that manipulating financial reporting to smooth earnings is becoming a business common practice (Burgstahler et al., 2006; Liu and Espahbodi, 2014). The empirical results suggest that pension plan reporting readability is a significant determinant of earnings volatility.

Practical implications

As a practical implication, this study points out that manipulations of the pension reporting readability are not costless. It incurs the costs of earnings instability.

Social implications

This study indicates that the issuance of SFAS 158 makes firms more likely to engage in pension plan readability manipulation. As a result, it has policy implication that the regulator should consider how the policy change alters the firm financial reporting behavior.

Originality/value

The empirical results suggest that firms may be more likely to engage in obfuscating pension plan disclosure after FASB’s issuance of SFAS 158. This would further increase outside investors’ assessed variance for inside debts and earnings volatilities. When policymakers require firms to recognize their funded status in statement of financial position, they should consider the costs or benefits that the firm manager face and, therefore, how this policy change alter the firm financial reporting behavior.
Institutional characteristics, signaling variables and IPO initial returns
Authors: D. Sundarasen Sheela Devi
Published: March 2019

Purpose

This paper aims to provide empirical evidence on the extent of alteration institutional characteristics, i.e. legal origin and corruption levels, may have on the signaling effects of auditors’ reputation, underwriters’ reputation and ownership retention on initial public offering (IPO) initial returns in OECD countries.

Design/methodology/approach

Cross-sectional data composed of 6,182 IPOs from 30 OECD countries are used for 2003-2012. Ordinary least square with multiple linear regressions is used to test the hypotheses.

Findings

The findings indicate that the legal framework and corruption level of a country alters the signaling effects of underwriters’ reputation, auditors’ reputation and ownership retention in an IPO environment. These three variables mitigate information asymmetry, signal firm value to potential investors and ultimately decrease IPO initial returns. This relationship is more significant in the civil law countries. Corruption levels negatively moderate the relationship in the common law and Scandinavian civil law countries but have no significance in the German and French civil law countries, indicating the importance of the signaling variables in these two civil law countries.

Originality/value

This study examines the extent of the alterations that the legal framework and the corruption levels cause to the signaling relationship between auditors’ reputation, underwriters’ reputation and ownership retention on IPO initial returns in selected OECD countries.
Testing derivatives pricing models under higher-order moment swaps
Authors: Doffou Ako
Published: March 2019

Purpose

This paper aims to test three parametric models in pricing and hedging higher-order moment swaps. Using vanilla option prices from the volatility surface of the Euro Stoxx 50 Index, the paper shows that the pricing accuracy of these models is very satisfactory under four different pricing error functions. The result is that taking a position in a third moment swap considerably improves the performance of the standard hedge of a variance swap based on a static position in the log-contract and a dynamic trading strategy. The position in the third moment swap is taken by running a Monte Carlo simulation.

Design/methodology/approach

This paper undertook empirical tests of three parametric models. The aim of the paper is twofold: assess the pricing accuracy of these models and show how the classical hedge of the variance swap in terms of a position in a log-contract and a dynamic trading strategy can be significantly enhanced by using third-order moment swaps. The pricing accuracy was measured under four different pricing error functions. A Monte Carlo simulation was run to take a position in the third moment swap.

Findings

The results of the paper are twofold: the pricing accuracy of the Heston (1993) model and that of two Levy models with stochastic time and stochastic volatility are satisfactory; taking a position in third-order moment swaps can significantly improve the performance of the standard hedge of a variance swap.

Research limitations/implications

The limitation is that these empirical tests are conducted on existing three parametric models. Maybe more critical insights could have been revealed had these tests been conducted in a brand new derivatives pricing model.

Originality/value

This work is 100 per cent original, and it undertook empirical tests of the pricing and hedging accuracy of existing three parametric models.
Reproductive effort influences intra‐seasonal variation in parasite‐specific antibody responses in wild Soay sheep
Authors: Hayward Adam D. , Pilkington Jill G. , Wilson Kenneth , McNeilly Tom N. , Watt Kathryn A.
Published: March 2019

A trade‐off between reproduction and immune function has been suggested to potentially underpin between‐individual and genetic variation in reproductive strategy, immunity and life span, with potential consequences for host and parasite dynamics.

Previous studies in wild animal populations have shown that experimentally induced or natural variation in reproductive effort is negatively associated with general immune markers. Few studies, however, have tackled this question by measuring specific immune responses against prevalent pathogens, and only rarely has variation in immune measures been linked to parasite burden, making it impossible to draw conclusions about the functional relevance of covariation between reproductive effort and immune markers.

We collected faecal samples in a longitudinal manner from wild female Soay sheep across late pregnancy and early lactation and measured gastrointestinal nematode faecal egg count (FEC) and worm‐specific antibody responses.

Faecal egg count was highly repeatable, with c. 80% of variation due to between‐individual differences, while three isotypes of worm‐specific and total antibodies had moderate repeatability (range: 11%–43%). Females making a greater reproductive effort (those that reproduced, and especially those with heavier litters) showed a more rapid increase in FEC across the season, while non‐reproducing females and those producing lighter litters experienced lower FEC and had higher antibody levels.

Associations between antibodies and FEC were complex: worm‐specific immunoglobulin (Ig) G was negatively associated with FEC, while total IgM was positively associated, emphasizing the importance of measuring both immune markers and parasite burden in ecological studies.

Our results support the predicted trade‐off between reproductive effort and parasite‐specific immunity: high reproductive effort can limit the ability of individuals to defend themselves against prevalent parasites, with potential downstream consequences for fitness and parasite transmission.

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