bluuebunny/crossref_metadata_2025 huggingface dataset:
The institution of the local referendum in Eastern Europe: the organization and legal regulation
Authors: Черкасов Александр Игоревич
Published: February 2021

This article examines the characteristic features of the institution of the local referendum in Eastern Europe. The author believes that it is very important for states, which have relatively recently embarked upon the path of democratic development, after decades of communist domination, that the traditional representative institutions are supplemented by the structures of direct democracy, which can facilitate the more effective involvement of the population in the social and political life of the state, and introduce them to modern democratic values. It’s pointed out in the article that attitudes towards the institution of the local referendum in the countries of Eastern Europe are ambiguous, and its introduction has run into a number of obstacles. This might be explained by institutional reasons, by citizens having limited interest in municipal affairs, and the absence of the tradition of citizens directly participating in the resolving of issues of governance. The institution of direct democracy has often only been introduced slowly, which is often due to resistance from the main political parties and the current political elites, who see the activation of direct democracy as a threat to their position. Nevertheless, the author stresses the important practical role of the institution of the local referendum, its ability to serve the interests of both the state as well as the citizens. Through to the institution of the referendum citizens have acquired an additional channel of participation in the political decision-making process, and this facilitates the realization of the principle of political equality and the formation of more open political power structures. The institution of the local referendum is after all an important instrument of political control on the part of the civil society that is necessary for the further development of democratic statehood in the countries of Eastern Europe.
Quo vadis, V4? What is the potential of the Czech Republic to promote its interests within the framework of the V4?
Authors: Dubský Zbyněk , Kočí Kateřina
Published: February 2021

The original purpose of the Visegrad Group (VG or V4 – which includes Hungary, Poland, the Czech Republic and Slovakia) was primarily to support its member states’ accession to the EU and NATO, which it successfully achieved. However, the views on the current cooperation of four Central European countries differ. Some researchers believe that the V4 has transformed since 2004 into a viable project which has become even an inspirational model of cooperation for other regional groupings. According to them, and contrary to doubts about the continuation of the V4 project, membership of the EU has given the V4 a new impulse, and its agenda has been expanded into new areas of cooperation which included EU affairs. Therefore, the V4 operates now as a distinct regional grouping within the EU (i.e. positive input regarding their commitment in several Council presidencies). Others, however, suggest that V4 cooperation seems to be labelled as a defensive project, a coalition within the EU, which is against something (recently the prominent topics have revolved around migration issues) and that it could lead to the marginalisation of the group and thus reduce its importance at the EU level. While discussing the future role of the V4, the article will focus on the Czech Republic, and its potential to promote its interests within the framework of the V4, especially in the context of its current presidency (from 1 July 2019 to 30 June 2020).
The trajectory of the process of democratization in Poland in the light of theory, political praxis and external evaluation: 1989–2019
Authors: Kubas Sebastian
Published: February 2021

In 1989 Poland decided to implement a democratic regime, but its future was uncertain then. Today, after 30 years of the development of the process of democratization, one can say that the road to democracy is winding. The article analyzes the Polish example of the process of democratization between 1989–2019. It refers to existing theories of the process of democratization and the evaluation of democracy. Although Poland passed through the phases of the erosion of a non-democratic regime, transition and democratic consolidation, the situation after 2015 suggests that there are trends that can reverse the process of democratization. It will be argued that the institutional and behavioral aspects of the process of democratization in Poland cannot be perceived as a linear process.
The role of emergency institutes in countering the COVID-19 pandemic in the United States
Authors: Черкасов Александр Игоревич
Published: February 2021

This article deals with the role of emergency institutes in countering the COVID-19 pandemic in the United States. The author examines such types of emergency as Public Health Emergency, National Emergency, Major Disaster Regime and Emergency Regime. The entire layer of emergency legislation is analysed, including National Emergencies Act of 1976, Robert T. Stafford Disaster Relief and Emergency Assistance Act of 1988, Social Security Act of 1935, Public Health Service Act of 1944, Defense Production Act of 1950. Acts of the President of the United States and the Congress adopted directly during the pandemic and aimed at countering COVID-19 and protecting the rights of American citizens are considered as well. In conclusion it’s being argued that the formal availability of federal mechanisms of mobilization of assistance to state and local government in the conditions of emergency (especially in the sphere of public health protection) by itself doesn’t guarantee effective response of the state machine to this situation, adequate and timely funding of the corresponding efforts.
Institutionalisation and implementation of the selected participatory instruments. The case of Poland
Authors: Kołomycew Anna
Published: February 2021

The article traces the process of the institutionalisation of selected elements of the mechanisms of public participation, whose consequence was the unification of the rules of implementation and formalisation. The process, however, did not result in an increase in civic engagement on the part of the citizens of all the territorial units under consideration. The article presents the outcome of both quantitative and qualitative (in-depth interviews) research conducted by the author in Polish municipalities. The theoretical framework of the article is provided by ‘new institutionalism’, and especially by ‘rational choice institutionalism’. The structure of the article is as follows: the first part focuses on the principles of new institutionalism with reference to the mechanisms of public participation. The second part presents a succinct analysis of the step-by-step institutionalisation of selected participatory mechanisms that have ensued in recent years. The third part contains a methodological overview of empirical research, while the fourth, and final, part includes the outcome of the research and its interpretation.
Constitutional amendments in Poland between 1997–2019
Authors: Szczepański Dominik
Published: February 2021

The aim of this article is to analyze parliamentary and non-parliamentary amendments to the Constitution of Poland in the years 1997–2019. The solutions and proposals for amendments, which are in chronological order by parliamentary (Sejm) session, are analysed in detail. These years determined not only the scope of the changes undertaken but above all, they were the main reason for the increased and variable activity of political entities related to the presentation of a comprehensive vision of the form of ready-made drafts of the Polish Constitution.
Mayors and their interaction with municipal councils in the countries of Eastern Europe
Authors: Черкасов Александр Игоревич
Published: February 2021

This article deals with the institute of mayor and his interaction with municipal councils in the countries of Eastern Europe. The author points out that the mayor personifies the whole urban governance mechanism and acts as an arbitrator in the struggle of various interests coming into confrontation at the level of a modern city. The article contains analyses of the trend towards “presidentialization” of the local political system and decollectivization of the decision making process at the municipal level common for many modern cities. On the basis of popular support and expectations the mayor begins to increasingly squeeze positions of local councils in the municipal mechanism. Direct mayoral elections are becoming more common and increase the independence of the head of local administration from political parties and slightly reduce the role of the latter in the decision making process
Effect of Different Farming Practices on Lactic Acid Bacteria Content in Cow Milk
Authors: Bava Luciana , Zucali Maddalena , Tamburini Alberto , Morandi Stefano , Brasca Milena
Published: February 2021

The natural load of lactic acid bacteria (LAB) in milk is the basis of the production of raw milk cheeses, such as Grana Padano PDO. In the last decades, improvements in livestock hygiene management resulted in bulk cow milk with less than 20,000 colony forming units (CFU) of bacterial count, unable to ensure a sufficient supply of LAB, with a negative impact on cheese quality. This study investigated the relations between farm management practices and prevalence of different groups of bacteria in cow milk. Sixty-two intensive dairy farms located in Lombardy (Italy) where involved, most of them destined as milk for the production of Grana Padano. Season had no significant effect on the content of most of the bacterial groups, except for coliforms. A strong relation among standard plate count (SPC) and other bacterial groups was evidenced. Cluster analysis showed that the most productive farms applied a complete milking routine and produced milk with the lowest value of SPC, the lowest count of the other bacteria, including LAB, but the highest LAB/SPC. The study suggests that complexity of farming practices can affect the microbial population of milk.
Amelioration of Pet Overpopulation and Abandonment Using Control of Breeding and Sale, and Compulsory Owner Liability Insurance
Authors: Bernete Perdomo Eva , Araña Padilla Jorge E. , Dewitte Siegfried
Published: February 2021

Overpopulation and abandonment of pets are long-standing and burgeoning concerns that involve uncontrolled breeding and selling, illegal trafficking, overpopulation, and pet safety and well-being issues. Abandonment of pets creates numerous negative externalities and multimillion-dollar costs, in addition to severe consequences and problems concerning animal welfare (e.g., starvation, untreated disease, climatic extremes, uncertainty of rescue and adoption), ecological (e.g., invasive species and introduction of novel pathogens), public health and safety (e.g., risks to people from bites, zoonoses, or road hazards), and economic (e.g., financial burdens for governmental and nongovernmental organizations). These interwoven problems persist for several reasons, including the following: (1) lack of an efficient system for the prevention of abandonment and overpopulation, (2) lack of regulatory liability for pet owners, (3) lack of legal alternative to abandonment. This article proposes a novel comprehensive management system for amelioration of overpopulation and abandonment of pets aimed to tackle the current supply and demand dysfunction of the pet market and provide a legal alternative to abandonment.
Is the Urban Form a Driver of Heavy Metal Pollution in Road Dust? Evidence from Mexico City
Authors: Aguilera Anahi , Bautista-Hernández Dorian , Bautista Francisco , Goguitchaichvili Avto , Cejudo Rubén
Published: February 2021

Environmental pollution is a negative externality of urbanization and is of great concern due to the fact that it poses serious problems to human health. Pollutants, such as heavy metals, have been found in urban road dust; however, it is unclear whether the urban form has a role in its accumulation, mainly in cases where there is no dominant unique source. We collected 482 samples of road dust, we determined the concentrations of five heavy metals (Cr, Cu, Pb, Zn, and Ni) using inductively coupled plasma optical emission spectrometry (ICP-OES), and then we derived the pollution load index (PLI). After estimating the mostly anthropogenic origin of these pollutants based on global levels of reference, there were two main aims of this study. Firstly, to analyze the spatial correlation of heavy metals, and secondly, to identify the main factors that influenced the heavy metal concentrations in the road dust of Mexico City. We did this by using a spatial autocorrelation indicator (Global Moran’s I) and applying ordinary least squares (OLS) and spatial regression models. The results indicated low levels of positive spatial autocorrelation for all heavy metals. Most variables failed to detect any relationship with heavy metals. The median strip area in the roads had a weak (significance level of 90%) but consistent positive relationship with Cr, Cu, Ni, Pb, and the PLI. The distance to the airport had a weak (significance level of 90%) and inverse relationship with Pb. Manufacturing units were associated with an increase in Cu (significance level of 95%), while the entropy index was associated with an increase in Ni (significance level of 95%).
Analysis of Intratumoral Heterogeneity in Myelodysplastic Syndromes with Isolated del(5q) Using a Single Cell Approach
Authors: Acha Pamela , Palomo Laura , Fuster-Tormo Francisco , Xicoy Blanca , Mallo Mar , Manzanares Ana , Grau Javier , Marcé Silvia , Granada Isabel , Rodríguez-Luaces Marta , Diez-Campelo María , Zamora Lurdes , Solé Francesc
Published: February 2021

Myelodysplastic syndromes (MDS) are a heterogeneous group of hematological diseases. Among them, the most well characterized subtype is MDS with isolated chromosome 5q deletion (MDS del(5q)), which is the only one defined by a cytogenetic abnormality that makes these patients candidates to be treated with lenalidomide. During the last decade, single cell (SC) analysis has emerged as a powerful tool to decipher clonal architecture and to further understand cancer and other diseases at higher resolution level compared to bulk sequencing techniques. In this study, a SC approach was used to analyze intratumoral heterogeneity in four patients with MDS del(5q). Single CD34+CD117+CD45+CD19- bone marrow hematopoietic stem progenitor cells were isolated using the C1 system (Fluidigm) from diagnosis or before receiving any treatment and from available follow-up samples. Selected somatic alterations were further analyzed in SC by high-throughput qPCR (Biomark HD, Fluidigm) using specific TaqMan assays. A median of 175 cells per sample were analyzed. Inferred clonal architectures were relatively simple and either linear or branching. Similar to previous studies based on bulk sequencing to infer clonal architecture, we were able to observe that an ancestral event in one patient can appear as a secondary hit in another one, thus reflecting the high intratumoral heterogeneity in MDS del(5q) and the importance of patient-specific molecular characterization.
Predicting Prognosis of Breast Cancer Patients with Brain Metastases in the BMBC Registry—Comparison of Three Different GPA Prognostic Scores
Authors: Riecke Kerstin , Müller Volkmar , Weide Rudolf , Schmidt Marcus , Park-Simon Tjoung-Won , Möbus Volker , Mundhenke Christoph , Polasik Arkadius , Lübbe Kristina , Hesse Tobias , Laakmann Elena , Thill Marc , A. Fasching Peter , Denkert Carsten , Fehm Tanja , Nekljudova Valentina , Rey Julia , Loibl Sibylle , Witzel Isabell
Published: February 2021

Several scores have been developed in order to estimate the prognosis of patients with brain metastases (BM) by objective criteria. The aim of this analysis was to validate all three published graded-prognostic-assessment (GPA)-scores in a subcohort of 882 breast cancer (BC) patients with BM in the Brain Metastases in the German Breast Cancer (BMBC) registry. The median age at diagnosis of BM was 57 years. All in all, 22.3% of patients (n = 197) had triple-negative, 33.4% (n = 295) luminal A like, 25.1% (n = 221) luminal B/HER2-enriched like and 19.2% (n = 169) HER2 positive like BC. Age ≥60 years, evidence of extracranial metastases (ECM), higher number of BM, triple-negative subtype and low Karnofsky-Performance-Status (KPS) were all associated with worse overall survival (OS) in univariate analysis (p < 0.001 each). All three GPA-scores were associated with OS. The breast-GPA showed the highest probability of classifying patients with survival above 12 months in the best prognostic group (specificity 68.7% compared with 48.1% for the updated breast-GPA and 21.8% for the original GPA). Sensitivities for predicting 3 months survival were very low for all scores. In this analysis, all GPA-scores showed only moderate diagnostic accuracy in predicting the OS of BC patients with BM.
In Vitro Response of Polyscias filicifolia (Araliaceae) Shoots to Elicitation with Alarmone–Diadenosine Triphosphate, Methyl Jasmonate, and Salicylic Acid
Authors: Śliwińska Anita , Naliwajski Marcin R. , Pietrosiuk Agnieszka , Sykłowska-Baranek Katarzyna
Published: February 2021

The effectiveness of different elicitation variants in combination with alarmone application was studied in shoot cultures of Polyscias filicifolia. The shoots were elicited with 200 µM methyl jasmonate (MeJA) or 50 µM salicylic acid (SA) alone or in combination, and their activity was compared with those treated with the alarmone diadenosine 5′,5‴-P1P3-triphosphate (Ap3A), either alone or in combination with SA and/or MeJA. All treatments resulted in significant stimulation of phenolic acid production (chlorogenic and ferulic acids), as well as oleanolic acid (OA) compared to control, with their highest concentration noted under simultaneous elicitation with SA and MeJA. While the maximum content of caffeic acid was detected after treatment with alarmone alone. In each of the culture variants enhanced antioxidant activity was observed, however the level varied according to the treatment. In addition, the SA, Ap3A and Ap3A+SA variants demonstrated additional peroxidase isoforms, as indicated by Native-PAGE, as well as the highest α-tocopherol content. The highest antioxidant capacity of shoot extracts was correlated with the highest abundance of phenolic compounds and OA. The results indicate that ROS induction appears to participate in the signal transduction following Ap3A treatment.
Zebrafish Models of Autosomal Dominant Ataxias
Authors: Quelle-Regaldie Ana , Sobrido-Cameán Daniel , Barreiro-Iglesias Antón , Sobrido María Jesús , Sánchez Laura
Published: February 2021

Hereditary dominant ataxias are a heterogeneous group of neurodegenerative conditions causing cerebellar dysfunction and characterized by progressive motor incoordination. Despite many efforts put into the study of these diseases, there are no effective treatments yet. Zebrafish models are widely used to characterize neuronal disorders due to its conserved vertebrate genetics that easily support genetic edition and their optic transparency that allows observing the intact CNS and its connections. In addition, its small size and external fertilization help to develop high throughput assays of candidate drugs. Here, we discuss the contributions of zebrafish models to the study of dominant ataxias defining phenotypes, genetic function, behavior and possible treatments. In addition, we review the zebrafish models created for X-linked repeat expansion diseases X-fragile/fragile-X tremor ataxia. Most of the models reviewed here presented neuronal damage and locomotor deficits. However, there is a generalized lack of zebrafish adult heterozygous models and there are no knock-in zebrafish models available for these diseases. The models created for dominant ataxias helped to elucidate gene function and mechanisms that cause neuronal damage. In the future, the application of new genetic edition techniques would help to develop more accurate zebrafish models of dominant ataxias.
Digital Immunophenotyping Predicts Disease Free and Overall Survival in Early Stage Melanoma Patients
Authors: De Logu Francesco , Galli Francesca , Nassini Romina , Ugolini Filippo , Simi Sara , Cossa Mara , Miracco Clelia , Gianatti Andrea , De Giorgi Vincenzo , Rulli Eliana , Cossu Antonio , Massi Daniela , Mandalà Mario
Published: February 2021

Background: the prognostic significance of tumor infiltrating lymphocytes (TILs) in intermediate/thick primary cutaneous melanoma (PCM) remains controversial, partially because conventional evaluation is not reliable, due to inter-observer variability and diverse scoring methods. We aimed to assess the prognostic impact of the density and spatial distribution of immune cells in early stage intermediate/thick PCM. Materials and Methods: digital image acquisition and quantitative analysis of tissue immune biomarkers (CD3, CD4, CD8, CD68, PD-L1, CD163, FOX-P3, and PD-1) was carried out in a training cohort, which included patients with primary PCM ≥ 2 mm diagnosed, treated, and followed-up prospectively in three Italian centers. Results were validated in an independent Italian cohort. Results: in the training cohort, 100 Stage II–III melanoma patients were valuable. At multivariable analysis, a longer disease free survival (DFS) was statistically associated with higher levels of CD4+ intratumoral T-cells (aHR [100 cell/mm2 increase] 0.98, 95%CI 0.95–1.00, p = 0.041) and CD163+ inner peritumoral (aHR [high vs. low] 0.56, 95%CI 0.32–0.99, p = 0.047). A statistically significant longer DFS (aHR [high-high vs. low-low] 0.52, 95%CI 0.28–0.99, p = 0.047) and overall survival (OS) (aHR [high-high vs. low-low] 0.39, 95%CI 0.18–0.85, p = 0.018) was found in patients with a high density of both intratumoral CD8+ T-cells and CD68+ macrophages as compared to those with low density of both intratumoral CD8+ T-cells and CD68+ macrophages. Consistently, in the validation cohort, patients with high density of both intratumoral CD8+ and CD3+ T-cells were associated to a statistically better DFS (aHR[high-high vs. low-low] 0.24, 95%CI 0.10–0.56, p < 0.001) and those with high density of both intratumoral CD8+ and CD68+ were associated to a statistically longer OS (aHR[high-high vs. low-low] 0.28, 95%CI 0.09–0.86, p = 0.025). Conclusion: our findings suggest that a specific preexisting profile of T cells and macrophages distribution in melanomas may predict the risk of recurrence and death with potential implications for the stratification of stage II–III melanoma patients.
Beneficial Roles of Cellulose Patch-Mediated Cell Therapy in Myocardial Infarction: A Preclinical Study
Authors: Simeoni Rossana , Mogharbel Bassam , Francisco Julio , Miyague Nelson , Irioda Ana , Souza Carolina , Souza Daiany , Stricker Priscila , da Rosa Nádia , Souza Clayton , Franco Celia , Sierakowski Maria-Rita , Abdelwahid Eltyeb , Guarita-Souza Luiz , Carvalho Katherine
Published: February 2021

Biological scaffolds have become an attractive approach for repairing the infarcted myocardium and have been shown to facilitate constructive remodeling in injured tissues. This study aimed to investigate the possible utilization of bacterial cellulose (BC) membrane patches containing cocultured cells to limit myocardial postinfarction pathology. Myocardial infarction (MI) was induced by ligating the left anterior descending coronary artery in 45 Wistar rats, and patches with or without cells were attached to the hearts. After one week, the animals underwent echocardiography to assess for ejection fraction and left ventricular end-diastolic and end-systolic volumes. Following patch formation, the cocultured cells retained viability of >90% over 14 days in culture. The patch was applied to the myocardial surface of the infarcted area after staying 14 days in culture. Interestingly, the BC membrane without cellular treatment showed higher preservation of cardiac dimensions; however, we did not observe improvement in the left ventricular ejection fraction of this group compared to coculture-treated membranes. Our results demonstrated an important role for BC in supporting cells known to produce cardioprotective soluble factors and may thus provide effective future therapeutic outcomes for patients suffering from ischemic heart disease.
Long-Term Atmospheric Aging and Corrosion of Epoxy Primer-Coated Aluminum Alloy in Coastal Environments
Authors: Zhang Tianyu , Zhang Teng , He Yuting , Zhang Sheng , Ma Binlin , Gao Zhigang
Published: February 2021

Aircraft are subjected to extreme weather conditions in coastal areas. This study reports long-term atmospheric exposure tests carried out on an epoxy primer-coated aluminum alloy in a coastal environment for 7, 12, and 20 years. The micromorphology and characteristics of the section and surface, the products of corrosion, electrochemical impedance, and molecular structure of the coated specimens were examined through a spectrophotometer, scanning electron microscopy (SEM), atomic force microscopy (AFM), X-ray diffraction (XRD), electrochemical impedance spectroscopy (EIS), and X-ray photoelectron spectrometer (XPS). The results showed that the angles of contact of the specimens with different numbers of years of atmospheric exposure satisfied the normal distribution. Their fractal dimensions increased with an increase in the duration of exposure. Intergranular corrosion and exfoliation corrosion appeared in the specimens after 20 years, where the product of corrosion was Al(OH)3. The impedances and thermal properties of the epoxy coatings were influenced by the synergistic effects of aging and post-curing. The impedances of the coatings decreased greatly after long-term atmospheric exposure. After 20 years of corrosion, the specimen showed the characteristics of the substrate being corroded. The mechanism of corrosion and the electrochemical equivalent circuit were also analyzed.
Polymer Coating Effects: Study of Material Properties and Architectural Application Characteristics of Aluminum Template
Authors: Hung Fei Shuo
Published: February 2021

In construction process, the formwork must be in contact with concrete to help the concrete solidify and fix the shape. Coating the formwork with a polymer can prolong its service life by reducing the amount of concrete sticking to the mold. Herein, an aluminum template substrate was coated with polyvinylidene difluoride (PVDF) or polyurethane (PU). Aluminum template material analysis was conducted, polymer film thickness was measured, and weather, moisture, pollution, salt spray, abrasion, impact, and acid and alkali resistance tests were conducted, as were tensile, bending, adhesion, hardness, and salt water resistance tests. Cement adhesion resistance was repeatedly tested. The experimental results indicated that the PVDF-coated template was superior. The novel PVDF Aluminum template exhibited high corrosion resistance and can be used in building materials, for example, in ceilings, partition walls, curtain walls, roof panels, and roof trusses. For reference, it can also be applied to ship structures and seaside and wind power generation projects.
Ultrafast Laser-Induced Crystallization of Lead Germanate Glass
Authors: Lotarev Sergey V. , Lipatiev Alexey S. , Lipateva Tatiana O. , Lopatina Elena V. , Sigaev Vladimir N.
Published: February 2021

Laser-induced space-selective crystallization of glass enabling the growth of continuous crystal-in-glass architectures consisting of non-centrosymmetric phases with functional properties is promising, including single-crystal waveguides for the development of integrated optical circuits. In this study, femtosecond laser direct writing of crystalline lines inside lead germanate glass with the composition close to Pb5Ge3O11 has been demonstrated. The growth of crystalline lines by the .moving focused laser beam required the preliminary growth of a seed crystal by the fixed beam. Confocal Raman spectroscopy revealed the precipitation of ferroelectric Pb5Ge3O11, which, under certain exposure conditions, could be accompanied by precipitation of the metastable lead germanate phase. Depending on the laser beam parameters, either bilateral growth providing split, horseshoe-shaped morphology of the crystal cross-section, or centered growth resulting in elongated, elliptical cross-section shape occurred. The obtained results are of interest for the fabrication of ferroelectric Pb5Ge3O11-based crystal-in-glass waveguides.
High-Durability Concrete with Supplementary Cementitious Admixtures Used in Corrosive Environments
Authors: Liu Shiming , Zhu Miaomiao , Ding Xinxin , Ren Zhiguo , Zhao Shunbo , Zhao Mingshuang , Dang Juntao
Published: February 2021

Durability of concrete is of great significance to prolong the service life of concrete structures in corrosive environments. Aiming at the economical and environment-friendly production of concrete by comprehensive utilization of the supplementary cementitious materials made of industrial byproducts, the resistances to chloride penetration, sulfate attack, and frost of high-performance concrete were studied in this paper. Fifteen concretes were designed at different water–binder ratio with the changes of contents of fly ash (FA), silica fume (SF), ground granulated blast-furnace slag (GGBS), and admixture of sulfate corrosion-resistance (AS). The compressive strength, the total electric flux of chloride penetrability, the sulfate resistance coefficient, and the indices of freezing and thawing were measured. Results indicate that, depending on the chemical composition, fineness, and pozzolanic activity, the supplementary cementitious admixtures had different effects on the compressive strength and the durability of concrete; despite having a higher fineness and pozzolanic activity, the GGBS gave out a negative effect on concrete due to a similar chemical composition with cement; the SF and FA presented beneficial effects on concrete whether they were used singly with GGBS or jointly with GGBS; the AS improved the compressive strength and the sulfate corrosion resistance of concrete. In general, the grade of durability was positively related to the compressive strength of concrete. Except for the concretes admixed only with GGBS or with GGBS and FA, others had super durability with the compressive strength varying from 70 MPa to 113 MPa. The concretes with water to binder ratio of 0.29 and total binders of 500 kg/m3 admixed with 7% FA + 8% SF + 8% GGBS or 7% FA + 8% SF + 8% GGBS + (10~12)% AS presented the highest grades of resistances specified in China codes to chloride penetration, sulfate corrosion, and frost, while the compressive strength was about 100 MPa.
A Histone Acetylation Modulator Gene Signature for Classification and Prognosis of Breast Cancer
Authors: Long Mengping , Hou Wei , Liu Yiqiang , Hu Taobo
Published: February 2021

Regulators of histone acetylation are promising epigenetic targets for therapy in breast cancer. In this study, we comprehensively analyzed the expression of histone acetylation modulator genes in breast cancer using TCGA data sources. A gene signature composed of eight histone acetylation modulators (HAMs) was found to be effective for the classification and prognosis of breast cancers, especially in the HER2-enriched and basal-like molecular subtypes. The eight genes consist of two histone acetylation writers (GTF3C4 and CLOCK), two erasers (HDAC2 and SIRT7) and four readers (BRD4, BRD7, SP100, and BRWD3). Both histone acetylation writer genes and eraser genes were found to be differentially expressed between the two groups indicating a close relationship exists between overall histone acetylation level and prognosis of breast cancer in HER2-enriched and basal-like breast cancer.
Entropy Pair Functional Theory: Direct Entropy Evaluation Spanning Phase Transitions
Authors: Nicholson Donald M. , Gao C. Y. , McDonnell Marshall T. , Sluss Clifton C. , Keffer David J.
Published: February 2021

We prove that, within the class of pair potential Hamiltonians, the excess entropy is a universal, temperature-independent functional of the density and pair correlation function. This result extends Henderson’s theorem, which states that the free energy is a temperature dependent functional of the density and pair correlation. The stationarity and concavity of the excess entropy functional are discussed and related to the Gibbs–Bugoliubov inequality and to the free energy. We apply the Kirkwood approximation, which is commonly used for fluids, to both fluids and solids. Approximate excess entropy functionals are developed and compared to results from thermodynamic integration. The pair functional approach gives the absolute entropy and free energy based on simulation output at a single temperature without thermodynamic integration. We argue that a functional of the type, which is strictly applicable to pair potentials, is also suitable for first principles calculation of free energies from Born–Oppenheimer molecular dynamics performed at a single temperature. This advancement has the potential to reduce the evaluation the free energy to a simple modification to any procedure that evaluates the energy and the pair correlation function.
Forecasting of Energy Demands for Smart Home Applications
Authors: Bhatt Dhowmya , D Danalakshmi , Hariharasudan A. , Lis Marcin , Grabowska Marlena
Published: February 2021

The utilization of energy is on the rise in current trends due to increasing consumptions by households. Smart buildings, on the other hand, aim to optimize energy, and hence, the aim of the study is to forecast the cost of energy consumption in smart buildings by effectively addressing the minimal energy consumption. However, smart buildings are restricted, with limited power access and capacity associated with Heating, Ventilation and Air Conditioning (HVAC) units. It further suffers from low communication capability due to device limitations. In this paper, a balanced deep learning architecture is used to offer solutions to address these constraints. The deep learning algorithm considers three constraints, such as a multi-objective optimization problem and a fitness function, to resolve the price management problem and high-level energy consumption in HVAC systems. The study analyzes and optimizes the consumption of power in smart buildings by the HVAC systems in terms of power loss, price management and reactive power. Experiments are conducted over various scenarios to check the integrity of the system over various smart buildings and in high-rise buildings. The results are compared in terms of various HVAC devices on various metrics and communication protocols, where the proposed system is considered more effective than other methods. The results of the Li-Fi communication protocols show improved results compared to the other communication protocols.
The Development of Strategies to Reduce Exhaust Emissions from Passenger Cars in Rzeszow City—Poland. A Preliminary Assessment of the Results Produced by the Increase of E-Fleet
Authors: Mądziel Maksymilian , Campisi Tiziana , Jaworski Artur , Tesoriere Giovanni
Published: February 2021

Urban agglomerations close to road infrastructure are particularly exposed to harmful exhaust emissions from motor vehicles and this problem is exacerbated at road intersections. Roundabouts are one of the most popular intersection designs in recent years, making traffic flow smoother and safer, but especially at peak times they are subject to numerous stop-and-go operations by vehicles, which increase the dispersion of emissions with high particulate matter rates. The study focused on a specific area of the city of Rzeszow in Poland. This country is characterized by the current composition of vehicle fleets connected to combustion engine vehicles. The measurement of the concentration of particulate matter (PM2.5 and PM10) by means of a preliminary survey campaign in the vicinity of the intersection made it possible to assess the impact of vehicle traffic on the dispersion of pollutants in the air. The present report presents some strategies to be implemented in the examined area considering a comparison of current and project scenarios characterized both by a modification of the road geometry (through the introduction of a turbo roundabout) and the composition of the vehicular flow with the forthcoming diffusion of electric vehicles. The study presents an exemplified methodology for comparing scenarios aimed at optimizing strategic choices for the local administration and also shows the benefits of an increased electric fleet. By processing the data with specific tools and comparing the scenarios, it was found that a conversion of 25% of the motor vehicles to electric vehicles in the current fleet has reduced the concentration of PM10 by about 30% along the ring road, has led to a significant reduction in the length of particulate concentration of the motorway, and it has also led to a significant reduction in the length of the particulate concentration for the access roads to the intersection.
Onboard Energy Storage and Power Management Systems for All-Electric Cargo Vessel Concept
Authors: Karkosiński Dariusz , Rosiński Wojciech Aleksander , Deinrych Piotr , Potrykus Szymon
Published: February 2021

This paper presents an innovative approach to the design of a forthcoming, fully electric-powered cargo vessel. This work begins by defining problems that need to be solved when designing vessels of this kind. Using available literature and market research, a solution for the design of a power management system and a battery management system for a cargo vessel of up to 1504 TEU capacity was developed. The proposed solution contains an innovative approach with three parallel energy sources. The solution takes into consideration the possible necessity for zero-emission work with the optional function of operation as an autonomous vessel. Energy storage system based on lithium-ion battery banks with a possibility of expanding the capacity is also described in this work as it is the core part of the proposed solution. It is estimated that the operation range for zero-emission work mode of up to 136 nautical miles can be achieved through the application of all fore-mentioned parts.
Integrating GIS-Based Point of Interest and Community Boundary Datasets for Urban Building Energy Modeling
Authors: Deng Zhang , Chen Yixing , Pan Xiao , Peng Zhiwen , Yang Jingjing
Published: February 2021

Urban building energy modeling (UBEM) is arousing interest in building energy modeling, which requires a large building dataset as an input. Building use is a critical parameter to infer archetype buildings for UBEM. This paper presented a case study to determine building use for city-scale buildings by integrating the Geographic Information System (GIS) based point-of-interest (POI) and community boundary datasets. A total of 68,966 building footprints, 281,767 POI data, and 3367 community boundaries were collected for Changsha, China. The primary building use was determined when a building was inside a community boundary (i.e., hospital or residential boundary) or the building contained POI data with main attributes (i.e., hotel or office building). Clustering analysis was used to divide buildings into sub-types for better energy performance evaluation. The method successfully identified building uses for 47,428 buildings among 68,966 building footprints, including 34,401 residential buildings, 1039 office buildings, 141 shopping malls, and 932 hotels. A validation process was carried out for 7895 buildings in the downtown area, which showed an overall accuracy rate of 86%. A UBEM case study for 243 office buildings in the downtown area was developed with the information identified from the POI and community boundary datasets. The proposed building use determination method can be easily applied to other cities. We will integrate the historical aerial imagery to determine the year of construction for a large scale of buildings in the future.
Impact of Inverter Based Resources on System Protection
Authors: Haddadi Aboutaleb , Farantatos Evangelos , Kocar Ilhan , Karaagac Ulas
Published: February 2021

Inverter-based resources (IBRs) exhibit different short-circuit characteristics compared to traditional synchronous generators (SGs). Hence, increased uptake of IBRs in the power system is expected to impact the performance of traditional protective relay schemes—set under the assumption of a SG-dominated power system. Protection engineers need to study these challenges and develop remedial solutions ensuring the effectiveness of system protection under higher levels of IBRs. To address this need, this paper studies the impact of IBRs on a variety of protective relay schemes including line distance protection, memory-polarized zero sequence directional protective relay element, negative sequence quantities-based protection, line current differential protection, phase comparison protection, rate-of-change-of-frequency, and power swing detection. For each protection function, potential misoperation scenarios are identified, and recommendations are provided to address the misoperation issue. The objective is to provide an improved understanding of the way IBRs may negatively impact the performance of traditional protection schemes as a first step towards developing future remedial solutions ensuring effective protection under high share of IBRs.
Blockchain-Enabled Edge Intelligence for IoT: Background, Emerging Trends and Open Issues
Authors: Du Yao , Wang Zehua , Leung Victor C. M.
Published: February 2021

Blockchain, a distributed ledger technology (DLT), refers to a list of records with consecutive time stamps. This decentralization technology has become a powerful model to establish trust among trustless entities, in a verifiable manner. Motivated by the recent advancement of multi-access edge computing (MEC) and artificial intelligence (AI), blockchain-enabled edge intelligence has become an emerging technology for the Internet of Things (IoT). We review how blockchain-enabled edge intelligence works in the IoT domain, identify the emerging trends, and suggest open issues for further research. To be specific: (1) we first offer some basic knowledge of DLT, MEC, and AI; (2) a comprehensive review of current peer-reviewed literature is given to identify emerging trends in this research area; and (3) we discuss some open issues and research gaps for future investigations. We expect that blockchain-enabled edge intelligence will become an important enabler of future IoT, providing trust and intelligence to satisfy the sophisticated needs of industries and society.
De Novo Development of mtDNA Deletion Due to Decreased POLG and SSBP1 Expression in Humans
Authors: Lee Yeonmi , Kim Taeho , Lee Miju , So Seongjun , Karagozlu Mustafa Zafer , Seo Go Hun , Choi In Hee , Lee Peter C. W. , Kim Chong-Jai , Kang Eunju , Lee Beom Hee
Published: February 2021

Defects in the mitochondrial genome (mitochondrial DNA (mtDNA)) are associated with both congenital and acquired disorders in humans. Nuclear-encoded DNA polymerase subunit gamma (POLG) plays an important role in mtDNA replication, and proofreading and mutations in POLG have been linked with increased mtDNA deletions. SSBP1 is also a crucial gene for mtDNA replication. Here, we describe a patient diagnosed with Pearson syndrome with large mtDNA deletions that were not detected in the somatic cells of the mother. Exome sequencing was used to evaluate the nuclear factors associated with the patient and his family, which revealed a paternal POLG mutation (c.868C > T) and a maternal SSBP1 mutation (c.320G > A). The patient showed lower POLG and SSBP1 expression than his healthy brothers and the general population of a similar age. Notably, c.868C in the wild-type allele was highly methylated in the patient compared to the same site in both his healthy brothers. These results suggest that the co- deficient expression of POLG and SSBP1 genes could contribute to the development of mtDNA deletion.
Health Anxiety Predicts the Perceived Dangerousness of COVID-19 over and above Intrusive Illness-Related Thoughts, Contamination Symptoms, and State and Trait Negative Affect
Authors: Sica Claudio , Caudek Corrado , Cerea Silvia , Colpizzi Ilaria , Caruso Maria , Giulini Paolo , Bottesi Gioia
Published: February 2021

This study sought to evaluate the specificity of health anxiety, relative to other forms of psychopathology, in perceptions of COVID-19 as dangerous. Measures of health anxiety, COVID-19 perceived dangerousness, negative affect, anxiety, depression, stress, contamination-related obsessions and compulsions, and intrusive illness-related thoughts were administered online to 742 community individuals during the Italian national lockdown. Results showed that, after controlling for demographic variables and other internalizing problems, health anxiety was the single most important factor associated with the perceived dangerousness of COVID-19. Moreover, a comparison between the current sample’s scores on various symptom measures and scores from prepandemic Italian samples revealed that, whereas other internalizing symptoms increased by a large or very large magnitude during the pandemic, levels of health anxiety and negative affect increased by a medium amount. This result may indicate that health anxiety is relatively trait-like, increasing the likelihood that our correlational data support the model of health anxiety as a vulnerability rather than an outcome. Together, these results indicate that health anxiety may be a specific risk factor for COVID-related maladjustment and support the distinction of health anxiety from other psychological problems.
Concurrent and Longitudinal Relationships between Positive Youth Development Attributes and Adolescent Internet Addiction Symptoms in Chinese Mainland High School Students
Authors: Dou Diya , Shek Daniel T. L.
Published: February 2021

In view of growing adolescent Internet addiction (IA) in the global context, there is a great need to understand the predictors of IA and design related evidence-based intervention and prevention programs. This longitudinal study investigated the relationships between Positive Youth Development (PYD) attributes and IA problems and the mediating role of life satisfaction using a large sample of Chinese high school students (N = 2648). Separated by one academic year, students completed a questionnaire evaluating their adolescent psychosocial adjustment, including validated PYD and IA measures. Multiple regression and structural equation modeling analyses were used. Consistent with the theoretical predictions of PYD models, results revealed a significant negative influence of PYD attributes on IA symptoms concurrently and longitudinally. In particular, general PYD attributes, such as emotional competence, spirituality, and resilience, showed strong and stable protective effects against IA. Life satisfaction also served as a mediator of the influence of all measures of PYD attributes on IA symptoms. The study underlines the importance of PYD attributes in promoting adolescents’ life satisfaction and preventing IA, and thus contributes to the design and implementation of evidence-based intervention and prevention programs.