bluuebunny/crossref_metadata_2025 huggingface dataset:
Safety and efficacy of zero-fluoroscopy catheter ablation for paroxysmal supraventricular tachycardia in Chinese children
Authors: Cui Xiaoran , Li Ruibin , Zhou Wenli , Zhang Xiaohui , Wang Xiaoning , Zhang Jidong
Published: September 2022

Objectives

To compare the safety and efficacy of completely zero-fluoroscopy radiofrequency ablation (RFA) with that of conventional RFA guided by three-dimensional mapping in Chinese children with paroxysmal supraventricular tachycardia (PSVT).

Methods

The study had a single-center observational design and included 46 children aged 6–14 years who underwent RFA for PSVT at the Second Hospital of Hebei Medical University between March 2019 and September 2021. The children were divided according to whether they underwent zero-fluoroscopy RFA (zero-fluoroscopy group, n = 26) or routine RFA under X-ray guidance (conventional group, n = 20). Three-dimensional mapping was used in both groups. Baseline characteristics, total procedure time, RFA time, volume and duration of X-ray exposure, target mapping time, the immediate RFA success rate, incidence of complications, and recurrence rate were compared between the two groups.

Results

The children had a median age of 12 years (interquartile range 10, 13), 47.8% (22/46) were boys, and 52.2% (24/46) were girls. The mean body weight was 48.75 ± 15.26 kg. There was no significant between-group difference in the baseline data (P > 0.05). All children were followed up as outpatients at 1, 3, and 6 months postoperatively. The target mapping time was significantly longer in the zero-fluoroscopy group than in the conventional group (12.96 ± 2.24 min vs. 6.65 ± 2.56 min, P < 0.05); however, there was no significant between-group difference in the immediate success rate (100% vs. 100%), success rate at 6 months postoperatively (92.30% vs. 95.00%), complication rate (0% vs. 0.05%), recurrence rate (7.70% vs. 5.00%), RFA time (212.50 s vs. 214.00 s), or total procedure time (78.50 min vs. 74.00 min) (P > 0.05).

Conclusion

Zero-fluoroscopy catheter ablation can completely avoid fluoroscopy exposure in children without affecting the safety and efficacy of RFA.
Single-center experience with a unibody single-branched stent graft for zone 2 thoracic endovascular aortic repair
Authors: Kong Xiang , Ruan Peng , Yu Jiquan , Chu Tianshu , Gao Lei , Jiang Hui , Ge Jianjun
Published: September 2022

To provide an adequate proximal landing zone, left subclavian artery (LSA) reconstruction has become an important part of thoracic endovascular aortic repair (TEVAR). This study evaluates the short and medium term efficacy of a novel unibody single-branched stent graft for zone 2 TEVAR. Fifty-two patients (mean age, 56 ± 10.9 years; 42 men) with distal aortic arch lesions requiring LSA reconstruction received unibody single-branched stents from September 2019 to March 2021. Computed tomography angiography was performed 6, 12, and 24 months after surgery to observe stent morphology, branch patency, endoleaks, stent-related adverse events, and changes in the diameter of true and false lumens. All stents were deployed adequately, and the technical success rate was 100%. The mean operation time was 121.8 ± 47.0 min. The mean postoperative hospital stay was 6.2 ± 3.7 days, and the mean follow-up was 16.8 ± 5.2 months (range, 12–24 months). During follow-up, there were no deaths and complications such as stent displacement or fracture, stenosis, fracture, occlusion, and type Ia endoleaks. The patency rate of the branched segment was 100%. In 42 patients with aortic dissection (AD), the true lumen diameter of the aortic isthmus was 29.4 ± 2.9 mm after surgery, significantly larger than before surgery (20.6 ± 5.4 mm, P < 0.05). Postoperative aortic isthmus false lumen diameter was significantly smaller than that before operation (6.1 ± 5.2 mm vs. 16.0 ± 7.6 mm, P < 0.05). The new unibody single-branched stent for zone 2 TEVAR is safe and accurate, and its efficacy is good in the short and medium term.
Interannual variability of winter precipitation over the Lambert Glacier basin linked to the dipole pattern of sea surface temperature in the southern Indian Ocean
Authors: Yang Jiao , Liu Ting , Dou Tingfeng , Xiao Cunde
Published: September 2022

Variations in annual accumulated snowfall over the Antarctic ice sheet have a significant and direct impact on mean sea-level change. The interannual variability of the precipitation over coastal Antarctica adjacent to the southern Indian Ocean (SIO) cannot be totally explained by the dominant mode of atmospheric variability in the Southern Hemisphere. This study explores the possible contributions from sea surface temperature (SST) anomalies in SIO on the precipitation over East Antarctica. The results suggest that the winter precipitation in the Lambert Glacier basin (LGB) is closely related to the autumn SST variability in SIO without the influence of El Niño–Southern Oscillation. It is shown that the positive autumn SIO dipole (SIOD) of SST anomalies is usually followed by reduced precipitation in the following winter over the LGB region and vice versa. The positive (negative) autumn SIOD can persist into the winter and excite cyclonic (anticyclonic) circulation and deepen (weaken) SIO low in high latitude, corresponding to an enhanced northward (southward) wind anomaly in LGB and central SIO. This mechanism prevents (promotes) the transportation of warm and moist marine air to the LGB region and hence decreases (increases) the precipitation during the following winter.
Lexical and cohesive links in EFL learners' writing: Exploring the use of task based language teaching
Authors: Albelihi Hani Hamad M.
Published: September 2022

As English has become the preferred language for recording current innovation and technological breakthroughs, text is the essential medium through which EFL learners enhance their writing abilities. Desirable though it may be, the researcher's experience has shown that most male students have just rudimentary L2 writing skills and are unable to write coherent passages in English. This implies a chasm between learners' lexical and cohesive connections and earlier research has focused on ways and means to bridge this gap. However, the current study is ground-breaking in this field as it explores the efficacy of task-oriented training in filling this chasm. Using linguistic learning methods, the current research examines the efficacy of lexical and cohesive links in enhancing undergraduates' writing. The study sample comprises 35 learners from an intermediate EFL reading class who are exposed to an intervention lasting 15 weeks. Data analysis shows that in the post-test, the learners' grammatical and vocabulary skills are enhanced dramatically, particularly in the discourse analysis sections. Furthermore, during group work activities, (i) students are more engaged and motivated, and they acquire more knowledge about the language system, identification, cause and effect, “if” clause and the purpose and function of using the passages given; (ii) half of the students' grammar proficiency and use of lexical items was correct in the writing output. Finally, the study shows that the biggest obstacle that the students faced in their writings and which they struggle to master is the use of the “if” clause which only 13 students could finally master. In addition, the study shows that (73%) of the students could master the discursive linkers in their writings better than the lexical or grammatical elements. Ultimately, the present study offers practical consequences that EFL teachers may want to contemplate including in their future classes.
Optimizing estradiol level for gonadotrophin-releasing hormone antagonist initiation among patients with simple tubal factor infertility
Authors: Wang Yizhuo , Xu Xiuhua , Yang Ai-min , Zhang Jie , Luo Zhuo-ye , Han Yan , Cui Na , Li Qian , Shi Baojun , Zhao Zhi-ming , Hao Gui-min
Published: September 2022

Objective

The aim of this study is to investigate the optimal estradiol (E2) level on the day of gonadotropin-releasing hormone antagonist (GnRH-ant) initiation to maximize the clinical pregnancy rate (CPR) after fresh embryo transfer among patients with simple tubal factor infertility.

Methods

A retrospective cohort study was conducted in the Reproductive Medicine Center, the Second Hospital of Hebei Medical University. A total of 1,493 IVF-ET cycles of patients diagnosed with single tubal factor infertility from August 2016 to August 2021 were included and equally allocated into five distinct groups according to the quintile serum E2 levels on the day of GnRH-ant initiation. The five groups had similar baseline data except for antral follicle count.

Result(s)

The serum E 2 level on GnRH-ant initiation day was determined as an independent predictor of clinical pregnancy after adjusting for confounding factors such as age, infertility duration, body mass index, cycle number, antral follicle count, and the number of transferred embryos. Through smooth curve fitting, we found that, with the increase of serum E2 levels on the day of GnRH-ant initiation, CPR showed a trend of slight increase and then slight decrease. The maximal CPR was achieved when the serum E2 level on GnRH-ant initiation day was 498 pg/ml. When E2 was less than 498 pg/ml, the odds ratio (OR) of clinical pregnancy was 1.05 (95% CI: 1.00, 1.11, P = 0.0583). When E2 was greater than 498 pg/ml, the OR of clinical pregnancy was 0.97 (95% CI: 0.95, 0.98, P = 0.0003). Furthermore, CPR remained high when E2 was 436.8–658.6 pg/ml but declined significantly by more than 40% when E2 was ≥ 894.4 pg/ml (P < 0.05).

Conclusion(s)

The serum E2 level should be considered as an adjuvant parameter for GnRH-ant initiation. The best E2 value was 498 pg/ml, and GnRH-ant administration could be recommended to initiate when serum E2 was 436.8–658.6 pg/ml. If GnRH-ant was initiated when serum E2 was above 894.4 pg/ml, then the CPR after fresh embryo transfer may decline dramatically, and thus, cancellation of fresh embryo transfer and earlier initiation of GnRH-ant in future cycles should be considered.
Prenatal health behaviours as predictors of human placental lactogen levels
Authors: Garay Samantha M. , Sumption Lorna A. , John Rosalind M.
Published: September 2022

Placental lactogen (hPL) is a key hormone of pregnancy responsible for inducing maternal adaptations critical for a successful pregnancy. Low levels of placental lactogen have been associated with lower birth weight as well as symptoms of maternal depression and anxiety. Lower placental lactogen has been reported in women with higher body mass index (BMI) but it is unclear whether prenatal health behaviours predict hPL levels or if hPL is associated with infant weight outcomes. This study utilised data from the longitudinal Grown in Wales cohort, based in South Wales. Participants were recruited at the pre-surgical appointment for an elective caesarean section. This study incorporates data from recruitment, post-delivery and a 12 month follow-up. Measures of maternal serum hPL were available for 248 participants. Analysis included unadjusted and adjusted linear and binary regression. Unadjusted, prenatal smoking and a Health Conscious dietary pattern were associated with hPL levels, however this was lost on adjustment for BMI at booking, Welsh Index of Multiple Deprivation (WIMD) score and placental weight. When stratified by maternal BMI at booking, a Health Conscious dietary pattern remained associated with increased hPL levels in women with a healthy BMI (p=.024, B=.59. 95% CI=.08,1.11) following adjustment for WIMD score and placental weight. When adjusted for a wide range of confounders, maternal hPL was also associated with increased custom birthweight centiles (CBWC) (p=.014, B=1.64. 95% CI=.33,2.94) and increased odds of large for gestational age deliveries (p=<.001, Exp(B)=1.42. 95% CI=1.17,1.72). This study identified that consuming a Health Conscious dietary pattern in pregnancy was associated with increased hPL, within women of a healthy BMI. Moreover, higher hPL levels were associated with increased CBWC and increased odds of delivering a large for gestational age infant. This improves the current limited evidence surrounding the nature of hPL in these areas.
Laser etching-based surface wetting modulation of silicone rubber for triboelectric nanogenerator
Authors: Liang Chao , Zhang Liwen , Zhao Nan , Zhou Wei , Deng Xue , Zhou Yuguang , Hou Yuxin , Zhang Wenqiang
Published: September 2022

Silicone rubber is a widely used functional material whose lifetime and safety properties can be improved by achieving superhydrophobicity on the surface. However, there are few studies on the use of superhydrophobic surfaces as nano-energy devices. In this work, laser etching was used to achieve continuous regulation of the superhydrophobic to superhydrophilic state of the surface on silicone rubber. The modulation of wettability is attributed to laser manufactured micro-nanostructures, while the laser can be used to recover the damaged structures. Meanwhile, a triboelectric nanogenerator (TENG) is fabricated using superhydrophobic silicone rubber and drives the LED to light up. In short, this study provides a simple solution for TENG and its recycling.
Spatio–temporal evolution and factors of climate comfort for urban human settlements in the Guangdong–Hong Kong–Macau Greater Bay Area
Authors: Zhou Chunshan , Zhang Dahao , Cao Yongwang , Wang Yunzhe , Zhang Guojun
Published: September 2022

This study used both temperature-humidity and wind efficiency indices at three time-scale resolutions (year, season, and month) for the first time, to analyze the spatio–temporal evolution of urban climate comfort in the Guangdong–Hong Kong–Macau Greater Bay Area (GBA). The main factors affecting human-settlement climate comfort were elucidated and the annual changes in both indices used in the study area exhibited fluctuating growth from 2005 to 2020. Moreover, the annual growth of the temperature-humidity and wind efficiency indices in the southern cities of the GBA was relatively fast. In contrast, the annual growth of these indices in the northern cities of the GBA was relatively slow. Overall, the climate of the human-settlement environments in the GBA was the most comfortable in spring and autumn, and summer and winter were characterized by hot and cold climate conditions, respectively. We did not identify any prominent change in the climate comfort of spring and autumn from 2005 to 2020; however, the climate comfort degree deteriorated in summer and ameliorated in winter. On a monthly scale, the human-settlement environments in the GBA were the coldest in December and the hottest in July. The urban human settlements were cold in January and February, hot in May, June, August, and September, and the most comfortable in March, April, October, and November in 2020. We analyzed the factors affecting the climate comfort of human-settlement environments in the study area and found that elevation, gross industrial production, population scale, and construction land area were the most influential parameters. Notably, the impact of natural factors on the climate comfort of human-settlement environments was more significant than that of anthropogenic factors. Moreover, the related factors affected the temperature-humidity index more strongly than the wind efficiency index. Overall, our results provide data-driven guidelines for improving the climate comfort of urban human settlements in the GBA.
Review of online measurement techniques for chemical composition of atmospheric clusters and sub-20 nm particles
Authors: Zhang Kewei , Xu Zhengning , Gao Jian , Xu Zhuanghao , Wang Zhibin
Published: September 2022

The chemical composition of aerosol particles is crucial to understanding their formation and evolution in the atmosphere. However, very limited information is available for the chemical composition of ultrafine particles, particularly for nanoclusters and newly formed particles, due to the lack of valid analytical methods. This work reviews the online measurement techniques for characterizing the chemical composition of atmospheric clusters and sub-20 nm particles. The commonly used instrumentations are divided into two categories: direct techniques based on mass spectrometry and indirect measurement techniques mainly relying on the physical properties (e.g., hygroscopicity and volatility). The advantages of these techniques are compared and their limitations in the lab and field application are summarized. The combination of direct and indirect techniques is discussed, and this may provide more comprehensive understanding of chemical information of atmospheric clusters and particles. We propose that the newly developing instrumentations are needed to improve the collection efficiency for direct techniques, or the chemical resolution for indirect techniques. Future development should focus on obtaining simultaneous measurements of particle physical and chemical properties, which can be helpful in improving the accuracy of modeling and the understanding of particle formation and evolution.
Study of the mechanism of digitalization boosting urban low-carbon transformation
Authors: Xinfa Tang , Jinglin Li
Published: September 2022

The climate problem is severe and many urban climates are poor. To deal with a series of climate problems, low-carbon measures are indispensable. Digital development is becoming a new driving force for social development. Therefore, the development of urban low-carbon transformation should be based on digital construction. This paper explores the significance and theoretical mechanism of the research of the mechanism of digitalization boosting urban low-carbon transition. Theoretically, digitalization can reduce carbon emissions through mechanisms such as optimizing industrial structure, adjusting energy structure, improving government governance efficiency, and changing people’s way of production and life. Finally, from the perspective of carbon neutrality, this paper puts forward relevant countermeasures, from the following six aspects: building a carbon emission measurement and monitoring system, promoting the digital transformation of enterprises, increasing the research and development of new energy technologies, improving the carbon emission market trading system, optimizing the carbon emission administrative management system, and practicing the low-carbon concept.
Effects of hydrological regime on Taxodium ascendens plant decomposition and nutrient dynamics in the Three Gorges Reservoir riparian zone
Authors: Chen Zhangting , Song Hong , Arif Muhammad , Li Changxiao
Published: September 2022

Riparian plants are an integral part of the river ecosystem and have significant impacts on the water quality of the reservoir area. The special hydrological process of the riparian zone makes it possible to become a “source” or “sink” of organic matter and nutrients. This study quantifies the flooding period on the decomposition as well as nutrient dynamics of leaves from artificially regenerated Taxodium ascendens in the riparian zone of the Three Gorges Reservoir (TGR) in China. Five decomposition treatment groups were exposed to 10, 30, 60, 90, and 180 days of decomposition: conventional water (T1), mild drought stress (T2), saturated water content (T3), light flooding stress (T4), and severe flooding stress (T5). In T4 and T5, the decomposition rate of T. ascendens leaves was significantly higher than that of T1, T2, and T3. All the leaf C, N, P, and K concentrations at the end of the test were lower than their respective starting values. The concentrations of N and P in the overlying water decrease to various degrees due to decomposition. At the end of the study, the TN content was 1.34 times (T4) and 1.16 times (T5) higher than that of the control group, and the TP content was 3.97 times (T4) and 3.21 times (T5) higher than that of the control group. Leaf decomposition increases the content of N and P in the overlying water under flood conditions, which adversely affects the water environment. This study establishes a theoretical framework for understanding how hydrological processes affect leaf decomposition and nutrient release in riparian areas, providing a scientific basis for riparian zone management in TGR.
The effectiveness of a large protected area to conserve a global endemism hotspot may vanish in the face of climate and land-use changes
Authors: Wang Danyang , de Knegt Henrik J. , Hof Anouschka R.
Published: September 2022

Endemic vertebrates are a crucial component of biodiversity, yet face disproportionally high extinction risk as climate and land-use changes drive habitat loss. Large protected areas are therefore deemed necessary to mitigate biodiversity loss. In 2021, China’s Giant Panda National Park (GPNP, 27,134 km2) was established in one of the global endemism hotspots. In this study we ask the question whether this large national park is able to conserve the many threatened endemic vertebrates occurring in the region in the face of climate and land-use changes, in order to assess the long-term effectiveness of the GPNP. We used species distribution modeling techniques to project the distributions of 40 threatened terrestrial (and freshwater) endemic vertebrates under land-use and climate change scenarios SSP2–4.5, SSP3–7.0 and SSP5–8.5 in 2081–2100, and assessed the extent to which their distributions are covered by the GPNP, now and in the future. We found that by 2081–2100, two thirds of the threatened endemic vertebrates are predicted to lose part (15–79%, N = 4) of or (nearly) their entire (80–100% loss, N = 23) range under all three climate and land-use change scenarios. Consequently, fewer species are predicted to occur in the GPNP than at present. Our findings confirm the high vulnerability of threatened endemic species to climate and land-use changes, despite protected areas. Habitat loss due to climate and land-use changes elevate extinction risk of species in endemism hotspots across the globe. Urgent, widespread and intensified mitigation measures and adaptation measures are required at a landscape scale for effective conservation efforts in the future.
Injury study of the 6-year-old pediatric thorax and abdomen in frontal sled tests using different computational models
Authors: Li Haiyan , Lv Wenle , Hynčík Luděk , Zhou Bingbing , Zhao Hongqian , Cui Shihai , He Lijuan , Ruan Shijie
Published: September 2022

The correct use of a child restraint system (CRS) is an effective internationally recognized measure to protect the safety of child occupants which can reduce the probability of child road traffic accident deaths by 54–80%. Finite element (FE) analysis is one important method with which to study the protection of child occupants. The aim of this study was to investigate thoracic and abdominal injuries and the protective effect of CRS on child occupants in 6-year-old (6YO) children in a frontal sled test using different computational models. In this study, a verified FE model of a 6YO child occupant was placed in the FE model of a CRS with a three-point safety belt. In the simulation setup phase, the frontal sled simulation of the 6YO FE model was reconstructed by applying the AAMA pulse. Based on the simulation data of the Q6 dummy FE model (Q6) and the 6YO child Virthuman model (V6) from previous studies, the frontal sled test simulation of a verified 6YO child FE model with detailed anatomical structures (TUST IBMs 6YO) was carried out to analyze pediatric thorax and abdomen injuries under the same experimental conditions. According to the simulation results, the variation tendencies of the simulation responses such as chest acceleration and compression are consistent with each other, which can provide effective information for the design of a CRS. In addition, the simulation results of the TUST IBMs 6YO can provide a variety of simulation data, such as the maximum first principal strain value and nephogram, of the internal organs of the chest and abdomen, providing a theoretical basis for the performance analysis and later development of a CRS.
Pan-cancer analysis of CREB3L1 as biomarker in the prediction of prognosis and immunotherapeutic efficacy
Authors: Lin Zhengjun , Wu Yanlin , Xiao XunGang , Zhang Xianghong , Wan Jia , Zheng Tao , Chen Hongxuan , Liu Tang , Tang Xianzhe
Published: September 2022

Background: CAMP response element binding protein 3-like 1 (CREB3L1) has been indicated as a critical biomarker and can modulate multifaced behaviors of tumor cells in diverse cancers. However, a systematic assessment of CREB3L1 in pan-cancer is of absence, and the predictive value of CREB3L1 in cancer prognosis, the tumor immune microenvironment and the efficacy of immunotherapy remains unexplored.

Methods: CREB3L1 expression in 33 different cancer types was investigated using RNAseq data from The Cancer Genome Atlas (TCGA) database. The characteristics of CREB3L1 alternations were illustrated in cBioPortal database. The prognostic and clinicopathological value of CREB3L1 was analyzed through clinical data downloaded from the TCGA database. The potential role of CREB3L1 in the tumor immune microenvironment was illustrated by utilizing CIBERSORT and ESTIMATE algorithms, and TISIDB online database. The associations between CREB3L1 expression and tumor mutation burden (TMB), and microsatellite instability (MSI) were assessed by spearman’s rank correlation coefficient. Furthermore, Gene Set Enrichment Analysis (GSEA) was conducted to explore the potential biological functions and downstream pathways of CREB3L1 in different human cancers. The correlations of CREB3L1 expression with PD-1/PD-L1 inhibitors efficacy and drug sensitivity were also investigated.

Results: The expression of CREB3L1 was abnormally high or low in several different cancer types, and was also strictly associated with the prognosis of cancer patients. CREB3L1 expression levels have a strong relationship with infiltrating immune cells, including regulatory T cells, CD8+ T cells, macrophages, B naïve cells, dendritic cells and mast cells. CREB3L1 expression was also correlated with the expression of multiple immune-related biomolecules, TMB, and MSI in several cancers. Moreover, CREB3L1 had promising applications in predicting the immunotherapeutic benefits and drug sensitivity in cancer management.

Conclusions: Our results highlight the value of CREB3L1 as a predictive biomarker for the prognosis and immunotherapy efficacy in multiple cancers, and CREB3L1 seems to play key roles in the tumor immune microenvironment, suggesting the role of CREB3L1 as a promising biomarker for predicting the prognosis and immune-related signatures in diverse cancers.
Prognostic value of LECT2 and relevance to immune infiltration in hepatocellular carcinoma
Authors: Qin Jiangfeng , Sun Weijie , Zhang Hui , Wu Zihao , Shen Jiapei , Wang Wenhai , Wei Yuanyuan , Liu Yanyan , Gao Yufeng , Xu Honghai
Published: September 2022

Background: Previous studies have shown that Leukocyte cell-derived chemotaxin2 (LECT2) is associated with the development of HCC. However, there are still no studies with a comprehensive analysis of the role of LECT2 in hepatocellular carcinoma (HCC).

Methods: TCGA data sets were used to analyze the expression of LECT2 in HCC. In addition, the prognostic value of LECT2 in HCC was also investigated. DriverDBv3 was used to analyze the Mutation, CNV, and methylation profiles of LECT2. And, validated by immunohistochemistry in 72 HCC samples. The prognostic value of LECT2 and the correlation with clinicopathological features were analyzed. The GO/KEGG enrichment analysis of LECT2 co-expression and gene set enrichment analysis (GSEA) was performed using the R software package. The PPI interaction network was constructed by Search Tool for the Retrieval of Interacting Genes (STRING) database. Immune infiltration was estimated by the XCELL, TIMER, QUANTISEQ, MCPCOUNTER, EPIC, CIBERSORT abs and CIBERSORT algorithms, and Spearman was used to analyzing their correlation with LECT2. Moreover, we analyzed the correlation of LECT2 expression with immune checkpoint molecules and HLA genes. Finally, we analyzed the IC50 values of six chemotherapeutic drugs by the pRRophetic package.

Results: Reduced LECT2 expression levels found in HCC patients. Moreover, decreased levels of LECT2 were associated with poor overall survival, disease-free survival, disease-specific survival, and progression-free survival. Besides, methylation was significantly associated with LECT2 expression. The functional enrichment analysis revealed that LECT2 may affect HCC progression through various pathways such as JAK/STAT signaling pathway, cell cycle, and pathways in cancer. Additionally, the results showed that LECT2 expression was negatively correlated with immune infiltration of B cells, Neutrophil, Monocyte, Cancer-associated fibroblast, and Myeloid dendritic cell, and positively correlated with T cell CD8+ naive, Endothelial cell, and Hematopoietic stem cell. LECT2 expression was negatively correlated with multiple immune checkpoint molecules and HLA genes. Chemosensitivity analysis showed that chemosensitivity was lower in the LECT2 high expression group. We validated the prognostic value of LECT2 and analysis of clinicopathological features showed a lower TNM stage in the group with high expression of LECT2.

Conclusion: Low expression of LECT2 in HCC is closely associated with poor prognosis, LECT2 may have potential clinical applications due to its unique immunological effects.
A Gly684Ala substitution in the androgen receptor is the cause for azoospermia in a Chinese family with mild androgen insensitivity syndrome and normal hormone levels
Authors: Yuan Yuan , Xu Wen-Qing , Chen Ying , Luo Tao , Chen Hou-Yang
Published: September 2022

Androgen receptor gene (AR) is essential for male growth and fertility. Its mutations are responsible for androgen insensitivity syndrome (AIS) that usually shows the phenotype of azoospermia resulting in male infertility. This study reported the first case of mild AIS with complete normal serum hormones in a Chinese family. The proband referred for infertility because of azoospermia. His uncle and two cousins are both infertile and have azoospermia. Whole-exome sequencing in the genetic analyses showed that the proband carries a novel hemizygous AR missense mutation, NM_000044.6: c.2051G>C (p.Gly684Ala), in exon four within the ligand-binding domain. His mother and maternal aunt are heterozygous carriers, while his father and brother are wildtype, indicating that the mutation in the proband was inherited from his mother. This pattern is consistent with the genetic model of the X-linked recessive inheritance of AR in AIS pathogenesis. HOPE predicts that p.Gly684Ala increases the hydrophobicity of AR but does not change the AR conformation. PolyPhen-2 predicts that p.Gly684Ala is harmful. This study provides the new knowledge to understand the AR gene mutations in MAIS.
No evidence for widespread positive selection on double substitutions within codons in primates and yeasts
Authors: Belinky Frida , Bykova Anastassia , Yurchenko Vyacheslav , Rogozin Igor B.
Published: September 2022

Nucleotide substitutions in protein-coding genes can be divided into synonymous (S) and non-synonymous (N) ones that alter amino acids (including nonsense mutations causing stop codons). The S substitutions are expected to have little effect on function. The N substitutions almost always are affected by strong purifying selection that eliminates them from evolving populations. However, additional mutations of nearby bases can modulate the deleterious effect of single N substitutions and, thus, could be subjected to the positive selection. This effect has been demonstrated for mutations in the serine codons, stop codons and double N substitutions in prokaryotes. In all abovementioned cases, a novel technique was applied that allows elucidating the effects of selection on double substitutions considering mutational biases. Here, we applied the same technique to study double N substitutions in eukaryotic lineages of primates and yeast. We identified markedly fewer cases of purifying selection relative to prokaryotes and no evidence of codon double substitutions under positive selection. This is consistent with previous studies of serine codons in primates and yeast. In general, the obtained results strongly suggest that there are major differences between studied pro- and eukaryotes; double substitutions in primates and yeasts largely reflect mutational biases and are not hallmarks of selection. This is especially important in the context of detection of positive selection in codons because it has been suggested that multiple mutations in codons cause false inferences of lineage-specific site positive selection. It is likely that this concern is applicable to previously studied prokaryotes but not to primates and yeasts where markedly fewer double substitutions are affected by positive selection.
Genomic selection in United States dairy cattle
Authors: Wiggans George R. , Carrillo José A.
Published: September 2022

The genomic selection program for dairy cattle in the United States has doubled the rate of genetic gain. Since 2010, the average annual increase in net merit has been $85 compared to $40 during the previous 5 years. The number of genotypes has been rapidly increasing both domestically and internationally and reached over 6.5 million in 2022 with 1,134,593 submitted in 2021. Evaluations are calculated for over 50 traits. Feed efficiency (residual feed intake), heifer and cow livability, age at first calving, six health traits, and gestation length have been added in recent years to represent the economic value of selection candidates more accurately; work is underway to develop evaluations for hoof health. Evaluations of animals with newly submitted genotypes are calculated weekly. In April 2019, evaluations were extended to crossbreds; to support that effort, evaluations are initially calculated on an all-breed base and then blended by an estimated breed composition. For animals that are less than 90% of one breed, the evaluation is calculated by weighting contributions of each of the five major dairy breeds evaluated (Ayrshire, Brown Swiss, Guernsey, Holstein, and Jersey) by the breed proportion. Nearly 200,000 animals received blended evaluations in July 2022. Pedigree is augmented by using haplotype matching to discover maternal grandsires and great-grandsires. Haplotype analysis is also used to discover undesirable recessive conditions. In many cases, the causative variant has been identified, and results from a gene test or inclusion on a genotyping chip improves the accuracy of those determinations for the current 27 conditions reported. Recently discovered recessive conditions include neuropathy with splayed forelimbs in Jerseys, early embryonic death in Holsteins, and curly calves in Ayrshires. Techniques have been developed to support rapid searches for parent-progeny relationships and identical genotypes among all likely genotypes, which substantially reduces processing time. Work continues on using sequence data to discover additional informative single nucleotide polymorphisms and to incorporate those previously discovered. Adoption of genotyping by sequencing is expected to improve flexibility of marker selection. The success of the Council on Dairy Cattle Breeding in conducting the genetic evaluation program is the result of close cooperation with industry and research groups, including the United States Department of Agriculture, breed associations, genotyping laboratories, and artificial-insemination organizations.
Diabetes exacerbated sepsis-induced intestinal injury by promoting M1 macrophage polarization via miR-3061/Snail1 signaling
Authors: Tan Fang , Cao Yuling , Zheng Lei , Wang Tao , Zhao Shuhua , Chen Jiong , Pang Changji , Xia Weiyi , Xia Zhengyuan , Li Ningning , Chi Xinjin
Published: September 2022

Background

Macrophages play important roles in diabetes and sepsis-related intestinal injury. Accumulating evidence suggests that microRNAs (miRNAs) act as the fundamental link between macrophage polarization and tissue injury. However, the underlying mechanisms of miRNAs in regulating macrophage polarization–related intestinal injury under diabetes and sepsis conditions remain unclear.

Methods

The cecal ligation and puncture (CLP)–induced sepsis models were established in male wild-type (WT) and diabetic mice. Clodronate liposome was used to deplete macrophage. H&E staining, inflammatory cytokines [tumor necrosis factor–α (TNF-α), interleukin-1β (IL-1β), and IL-6], and intestinal mucosal barrier function markers [occludin, ZO-1, lipopolysaccharide (LPS), and intestinal fatty acid binding protein (iFABP)] were used to assess elevated intestinal damage. miRNA array, RNA-seq, and bioinformatic analysis were performed to detect the miRNA and messenger RNA (mRNA) expression and the potential regulation mechanism. In vitro, RAW264.7 cells were cultured in the absence or presence of high glucose and LPS, miR-3061 mimics, and Snail small interfering RNA stimulation, respectively, for further mechanism studies. Luciferase reporter assay was used to confirm the interplay between miRNA and its target genes.

Results

Compared with WT CLP mice, the diabetic CLP mice showed severe intestinal damage characterized by significant increases in Chui’s scores, expression of inflammatory cytokines (TNF-α, IL-1β, and IL-6), serum LPS and iFABP concentration, and significant reductions in tight junction protein occludin and ZO-1 levels. Macrophage depletion reversed the intestinal damage caused by CLP. The bioinformatic analysis revealed that miR-3061/Snail1 might be a potential regulation axis of macrophage polarization. Furthermore, high glucose and LPS stimulation increased M1 macrophage and reduced the levels of miR-3061, which was negatively associated with Snail1 in RAW264.7 cells. Mechanistic studies demonstrated that miR-3061 regulated macrophage polarization by targeting the Snail1 mRNA 3′‐untranslated region. Moreover, miR-3061 overexpression suppressed Snail1 expression and inhibited M1 macrophage and inflammatory cytokines.

Conclusion

This study elucidated that diabetes exacerbated sepsis-induced intestinal injury by promoting M1 macrophage polarization and further demonstrated that the miR-3061/Sani1 axis may be the potential target of macrophage polarization.
Case Report: Susceptibility to viral infections and secondary hemophagocytic lymphohistiocytosis responsive to intravenous immunoglobulin as primary manifestations of adenosine deaminase 2 deficiency
Authors: Drago Enrico , Garbarino Francesca , Signa Sara , Grossi Alice , Schena Francesca , Penco Federica , Santori Elettra , Candotti Fabio , Boztug Kaan , Volpi Stefano , Gattorno Marco , Caorsi Roberta
Published: September 2022

Deficiency of adenosine deaminase 2 (DADA2) is an autosomal recessive disease associated with a highly variable clinical presentation, including systemic vasculitis, immunodeficiency, and cytopenia. We report a case of a 16-year-old girl affected by recurrent viral infections [including cytomegalovirus (CMV)-related hepatitis and measles vaccine virus-associated manifestations] and persistent inflammation, which occurred after Parvovirus infection and complicated by secondary hemophagocytic lymphohistiocytosis (HLH). HLH’s first episode presented at 6 years of age and was preceded by persistent fever and arthralgia with evidence of Parvovirus B19 infection. The episode responded to intravenous steroids but relapsed during steroids tapering. High-dose intravenous immunoglobulin (IVIG) helped manage her clinical symptoms and systemic inflammation. The frequency of IVIG administration and the dosage were progressively reduced. At the age of 9, she experienced varicella zoster virus (VZV) reactivation followed by the recurrence of the inflammatory phenotype complicated by HLH with neurological involvement. Again, high-dose steroids and monthly IVIG resulted in a quick response. Targeted next-generation sequencing (NGS) for autoinflammatory diseases and immunodeficiencies revealed the homozygous Leu183Pro ADA2 mutation, which was confirmed by Sanger analysis. ADA2 enzymatic test showed a complete loss of ADA2 activity. For about 3 years, IVIG alone was completely effective in preventing flares of inflammation and neurological manifestations. Anti-TNF treatment was started at the age of 13 for the appearance of recurrent genital ulcers, with a complete response. This case further expands the clinical spectrum of DADA2 and emphasizes the importance of extensive genetic testing in clinical phenotypes characterized by persistent unspecific inflammatory syndromes. The use of high doses of IVIG might represent a possible effective immune modulator, especially in combination with anti-TNF treatment.
Efficacy and safety of dietary polyphenol supplementation in the treatment of non-alcoholic fatty liver disease: A systematic review and meta-analysis
Authors: Yang Kailin , Chen Junpeng , Zhang Tianqing , Yuan Xiao , Ge Anqi , Wang Shanshan , Xu Hao , Zeng Liuting , Ge Jinwen
Published: September 2022

Background

Dietary polyphenol treatment of non-alcoholic fatty liver disease (NAFLD) is a novel direction, and the existing clinical studies have little effective evidence for its therapeutic effect, and some studies have inconsistent results. The effectiveness of dietary polyphenols in the treatment of NAFLD is still controversial. The aim of this study was to evaluate the therapeutic efficacy of oral dietary polyphenols in patients with NAFLD.

Methods

The literature (both Chinese and English) published before 30 April 2022 in PubMed, Cochrane, Medline, CNKI, and other databases on the treatment of NAFLD with dietary polyphenols was searched. Manual screening, quality assessment, and data extraction of search results were conducted strictly according to the inclusion and exclusion criteria. RevMan 5.3 software was used to perform the meta-analysis.

Results

The RCTs included in this study involved dietary supplementation with eight polyphenols (curcumin, resveratrol, naringenin, anthocyanin, hesperidin, catechin, silymarin, and genistein) and 2,173 participants. This systematic review and meta-analysis found that 1) curcumin may decrease body mass index (BMI), Aspartate aminotransferase (AST), Alanine aminotransferase (ALT), Triglycerides (TG) total cholesterol (TC), and Homeostasis Model Assessment-Insulin Resistance (HOMA-IR) compared to placebo; and curcumin does not increase the occurrence of adverse events. 2) Although the meta-analysis results of all randomized controlled trials (RCTs) did not reveal significant positive changes, individual RCTs showed meaningful results. 3) Naringenin significantly decreased the percentage of NAFLD grade, TG, TC, and low-density lipoprotein cholesterol (LDL-C) and increased high-density lipoprotein cholesterol (HDL-C) but had no significant effect on AST and ALT, and it is a safe supplementation. 4) Only one team presents a protocol about anthocyanin (from Cornus mas L. fruit extract) in the treatment of NAFLD. 5) Hesperidin may decrease BMI, AST, ALT, TG, TC, HOMA-IR, and so on. 6) Catechin may decrease BMI, HOMA-IR, and TG level, and it was well tolerated by the patients. 7) Silymarin was effective in improving ALT and AST and reducing hepatic fat accumulation and liver stiffness in NAFLD patients.

Conclusion

Based on current evidence, curcumin can reduce BMI, TG, TC, liver enzymes, and insulin resistance; catechin can reduce BMI, insulin resistance, and TG effectively; silymarin can reduce liver enzymes. For resveratrol, naringenin, anthocyanin, hesperidin, and catechin, more RCTs are needed to further evaluate their efficacy and safety.
Prognostic stratification based on m5C regulators acts as a novel biomarker for immunotherapy in hepatocellular carcinoma
Authors: Liu Ping , Zhu Ziqing , Ma Jiayao , Wei Le , Han Ying , Shen Edward , Tan Xiao , Chen Yihong , Cai Changjing , Guo Cao , Peng Yinghui , Gao Yan , Liu Yongting , Huang Qiaoqiao , Gao Le , Li Yin , Jiang Zhaohui , Wu Wantao , Liu Yihan , Zeng Shan , Li Wei , Feng Ziyang , Shen Hong
Published: September 2022

Background

Immunotherapy is a promising anti-cancer strategy in hepatocellular carcinoma (HCC). However, a limited number of patients can benefit from it. There are currently no reliable biomarkers available to find the potential beneficiaries. Methylcytosine (m5C) is crucial in HCC, but its role in forecasting clinical responses to immunotherapy has not been fully clarified.

Methods

In this study, we analyzed 371 HCC patients from The Cancer Genome Atlas (TCGA) database and investigated the expression of 18 m5C regulators. We selected 6 differentially expressed genes (DEGs) to construct a prognostic risk model as well as 2 m5C-related diagnostic models.

Results

The 1-, 3-, and 5-year area under the curve (AUC) of m5C scores for the overall survival (OS) was 0.781/0.762/0.711, indicating the m5C score system had an ideal distinction of prognostic prediction for HCC. The survival analysis showed that patients with high-risk scores present a worse prognosis than the patients with low-risk scores (p< 0.0001). We got consistent results in 6 public cohorts and validated them in Xiangya real-world cohort by quantitative real-time PCR and immunohistochemical (IHC) assays. The high-m5C score group was predicted to be in an immune evasion state and showed low sensitivity to immunotherapy, but high sensitivity to chemotherapy and potential targeted drugs and agents, such as sepantronium bromide (YM-155), axitinib, vinblastine and docetaxel. Meanwhile, we also constructed two diagnostic models to distinguish HCC tumors from normal liver tissues or liver cirrhosis.

Conclusion

In conclusion, our study helps to early screen HCC patients and select patients who can benefit from immunotherapy. Step forwardly, for the less likely beneficiaries, this study provides them with new potential targeted drugs and agents for choice to improve their prognosis.
The emerging roles of TRIM21 in coordinating cancer metabolism, immunity and cancer treatment
Authors: Chen Xintian , Cao Menghan , Wang Pengfei , Chu Sufang , Li Minle , Hou Pingfu , Zheng Junnian , Li Zhongwei , Bai Jin
Published: September 2022

Tripartite motif containing-21 (TRIM21), an E3 ubiquitin ligase, was initially found to be involved in antiviral responses and autoimmune diseases. Recently studies have reported that TRIM21 plays a dual role in cancer promoting and suppressing in the occurrence and development of various cancers. Despite the fact that TRIM21 has effects on multiple metabolic processes, inflammatory responses and the efficacy of tumor therapy, there has been no systematic review of these topics. Herein, we discuss the emerging role and function of TRIM21 in cancer metabolism, immunity, especially the immune response to inflammation associated with tumorigenesis, and also the cancer treatment, hoping to shine a light on the great potential of targeting TRIM21 as a therapeutic target.