bluuebunny/crossref_metadata_2025 huggingface dataset:
Optimization Study on Surface Roughness and Tribological Behavior of Recycled Cast Iron Reinforced Bronze MMCs Produced by Hot Pressing
Authors: Güneş Aydın , Şahin Ömer Sinan , Düzcükoğlu Hayrettin , Salur Emin , Aslan Abdullah , Kuntoğlu Mustafa , Giasin Khaled , Pimenov Danil Yurievich
Published: June 2021

Surface roughness reflects the quality of many operational parameters, namely service life, wear characteristics, working performance and tribological behavior of the produced part. Therefore, tribological performance is critical for the components used as tandem parts, especially for the MMCs (Metal Matrix Composites) which are a unique class of materials having extensive application areas such as aerospace, aeronautics, marine engineering and the defense industry. Current work covers the optimization study of production parameters for surface roughness and tribological indicators of newly produced cast iron reinforced bronze MMCs. In this context, two levels of temperature (400 and 450 °C), three levels of pressure (480, 640 and 820 MPa) and seven levels of reinforcement ratios (60/40, 70/30, 80/20, 90/10, 100/0 of GGG40/CuSn10, pure bronze-as received and pure cast iron-as received) are considered. According to the findings obtained by Taguchi’s signal-to-noise ratios, the reinforcement ratio has a dominant effect on surface roughness parameters (Ra and Rz), the coefficient of friction and the weight loss in different levels. In addition, 100/0 reinforced GGG40/CuSn10 gives minimum surface roughness, pure cast iron provides the best weight loss and pure bronze offers the desired coefficient of friction. The results showed the importance of material ingredients on mechanical properties by comparing a wide range of samples from starting the production phase, which provides a perspective for manufacturers to meet the market supply as per human requirements.
Simulation of the Circulating Motion of the Working Medium and Metal Removal during Multi-Energy Processing under the Action of Vibration and Centrifugal Forces
Authors: Kundrák János , Mitsyk Andrey V. , Fedorovich Vladimir A. , Markopoulos Angelos P. , Grabchenko Anatoly I.
Published: June 2021

The rotational motion of the medium granules under the influence of an impeller installed in the bottom of a cylindrical reservoir is considered. The dependencies of the circulation velocity of the abrasive granules, as well as the dependence of the pressure in the circulation flow of the granules on the radius of the vibrating machine cylindrical reservoir for different speeds of the impeller rotation are obtained. Furthermore, the velocities of the abrasive granules at various distances from the center of the cylindrical reservoir of the vibrating machine have been determined. The amplitudes of the tangential and radial components of the velocity of movement of pseudo-gas from abrasive granules are obtained. The total pressure on the surface of the processed part and the average velocity of the abrasive granules colliding with it are determined. The graphical dependencies of the integral metal removal on the amplitude and frequency of oscillations of the walls of the vibrating machine reservoir are given for various values of the angular velocities of the impeller rotation.
An α-Monotone Generalized Log-Moyal Distribution with Applications to Environmental Data
Authors: Arslan Talha
Published: June 2021

Modeling environmental data plays a crucial role in explaining environmental phenomena. In some cases, well-known distributions, e.g., Weibull, inverse Weibull, and Gumbel distributions, cannot model environmental events adequately. Therefore, many authors tried to find new statistical distributions to represent environmental phenomena more accurately. In this paper, an α-monotone generalized log-Moyal (α-GlogM) distribution is introduced and some statistical properties such as cumulative distribution function, hazard rate function (hrf), scale-mixture representation, and moments are derived. The hrf of the α-GlogM distribution can form a variety of shapes including the bathtub shape. The α-GlogM distribution converges to generalized half-normal (GHN) and inverse GHN distributions. It reduces to slash GHN and α-monotone inverse GHN distributions for certain parameter settings. Environmental data sets are used to show implementations of the α-GlogM distribution and also to compare its modeling performance with its rivals. The comparisons are carried out using well-known information criteria and goodness-of-fit statistics. The comparison results show that the α-GlogM distribution is preferable over its rivals in terms of the modeling capability.
The Cădariu-Radu Method for Existence, Uniqueness and Gauss Hypergeometric Stability of Ω-Hilfer Fractional Differential Equations
Authors: Aderyani Safoura , Saadati Reza , Fečkan Michal
Published: June 2021

Using the Cădariu–Radu method derived from the Diaz–Margolis theorem, we study the existence, uniqueness and Gauss hypergeometric stability of Ω-Hilfer fractional differential equations defined on compact domains. Next, we show the main results for unbounded domains. To illustrate the main result for a fractional system, we present an example.
On Basic Probability Logic Inequalities
Authors: Joksimović Marija Boričić
Published: June 2021

We give some simple examples of applying some of the well-known elementary probability theory inequalities and properties in the field of logical argumentation. A probabilistic version of the hypothetical syllogism inference rule is as follows: if propositions A, B, C, A→B, and B→C have probabilities a, b, c, r, and s, respectively, then for probability p of A→C, we have f(a,b,c,r,s)≤p≤g(a,b,c,r,s), for some functions f and g of given parameters. In this paper, after a short overview of known rules related to conjunction and disjunction, we proposed some probabilized forms of the hypothetical syllogism inference rule, with the best possible bounds for the probability of conclusion, covering simultaneously the probabilistic versions of both modus ponens and modus tollens rules, as already considered by Suppes, Hailperin, and Wagner.
Optimization of the Continuous Casting Process of Hypoeutectoid Steel Grades Using Multiple Linear Regression and Genetic Programming—An Industrial Study
Authors: Brezocnik Miran , Župerl Uroš
Published: June 2021

Štore Steel Ltd. is one of the major flat spring steel producers in Europe. Until 2016 the company used a three-strand continuous casting machine with 6 m radius, when it was replaced by a completely new two-strand continuous caster with 9 m radius. For the comparison of the tensile strength of 41 hypoeutectoid steel grades, we conducted 1847 tensile strength tests during the first period of testing using the old continuous caster, and 713 tensile strength tests during the second period of testing using the new continuous caster. It was found that for 11 steel grades the tensile strength of the rolled material was statistically significantly lower (t-test method) in the period of using the new continuous caster, whereas all other steel grades remained the same. To improve the new continuous casting process, we decided to study the process in more detail using the Multiple Linear Regression method and the Genetic Programming approach based on 713 items of empirical data obtained on the new continuous casting machine. Based on the obtained models of the new continuous casting process, we determined the most influential parameters on the tensile strength of a product. According to the model’s analysis, the secondary cooling at the new continuous caster was improved with the installation of a self-cleaning filter in 2019. After implementing this modification, we performed an additional 794 tensile tests during the third period of testing. It was found out that, after installation of the self-cleaning filter, in 6 steel grades out of 19, the tensile strength in rolled condition improved statistically significantly, whereas all the other steel grades remained the same.
An Investigation into Accumulative Difference Mechanism in Time and Space for Material Removal in Micro-EDM Milling
Authors: Jing Qi , Zhang Yongbin , Kong Lingbao , Xu Min , Ji Fang
Published: June 2021

In micro-electrical discharge machining (micro-EDM) milling, the cross-section of the microgroove machine is frequently not an ideal rectangle. For instance, there are arc shapes on the bottom and corners, and the sidewall is not steep. The theoretical explanation for this phenomenon is still lacking. In addition to the tip discharge effect, the essential reason is that there is an accumulative difference in time and space during the shape change process of a tool electrode and the microstructure formation on a workpiece. The process parameters are critical influencing factors that determine this accumulative difference. Therefore, the accumulative difference mechanism in time and space is investigated in this paper, and then a theoretical model is developed to simulate the micro-EDM milling process with a straight-line single path. The simulation results for a cylindrical electrode at the two rotational speeds of 0 (nonrotating) and 300 rpm are compared, while the results for a cylindrical electrode and a square electrode at a rotation speed of 0 are also compared to verify that different process parameters generate accumulative differences in the time and space of material removal. Finally, micro-EDM milling experiments are carried out to verify the simulation model. The maximum mean relative deviation between the microgroove profiles of simulation results and those of experiments is 11.09%, and the profile shapes of simulations and experiments have a good consistency. A comparative experiment between a cylindrical electrode and a hollow electrode is also performed, which further verifies the mechanism revealed in the study. Furthermore, the cross-section profile of a microgroove can be effectively controlled by adjusting the process parameters when utilising these accumulative differences through fabricating a microgroove with a V-shaped cross-section by a square electrode and a microgroove with a semi-circular cross-section by a cylindrical electrode. This research provides theoretical guidance for solving the problems of the machining accuracy of detail features in micro-EDM milling, for instance, to machine a microgroove with an ideal rectangular cross-section.
Coordinate Induction of Humoral and Spike Specific T-Cell Response in a Cohort of Italian Health Care Workers Receiving BNT162b2 mRNA Vaccine
Authors: Agrati Chiara , Castilletti Concetta , Goletti Delia , Meschi Silvia , Sacchi Alessandra , Matusali Giulia , Bordoni Veronica , Petrone Linda , Lapa Daniele , Notari Stefania , Vanini Valentina , Colavita Francesca , Aiello Alessandra , Agresta Alessandro , Farroni Chiara , Grassi Germana , Leone Sara , Vaia Francesco , Capobianchi Maria , Ippolito Giuseppe , Puro Vincenzo ,   on behalf of the INMI COVID-190 Vaccine Study Group
Published: June 2021

Vaccination is the main public health measure to reduce SARS-CoV-2 transmission and hospitalization, and a massive worldwide scientific effort resulted in the rapid development of effective vaccines. This work aimed to define the dynamics of humoral and cell-mediated immune response in a cohort of health care workers (HCWs) who received a two-dose BNT162b2-mRNA vaccination. The serological response was evaluated by quantifying the anti-RBD and neutralizing antibodies. The cell-mediated response was performed by a whole blood test quantifying Th1 cytokines (IFN-γ, TNF-α, IL-2), produced in response to spike peptides. The BNT162b2-mRNA vaccine induced both humoral and cell-mediated immune responses against spike peptides in virtually all HCWs without previous SARS-CoV-2 infection, with a moderate inverse relation with age in the anti-RBD response. Spike-specific T cells produced several Th1 cytokines (IFN-γ, TNF-α, and IL-2), which correlated with the specific-serological response. Overall, our study describes the ability of the BNT162b2 mRNA vaccine to elicit a coordinated neutralizing humoral and spike-specific T cell response in HCWs. Assessing the dynamics of these parameters by an easy immune monitoring protocol can allow for the evaluation of the persistence of the vaccine response in order to define the optimal vaccination strategy.
Determination of Antimicrobial Resistance Patterns in Salmonella from Commercial Poultry as Influenced by Microbiological Culture and Antimicrobial Susceptibility Testing Methods
Authors: Wang Xi , Chaney W. Evan , Pavlidis Hilary O. , McGinnis James P. , Byrd J. Allen , Farnell Yuhua Z. , Johnson Timothy J. , McElroy Audrey P. , Farnell Morgan B.
Published: June 2021

Monitoring antimicrobial resistance of foodborne pathogens in poultry is critical for food safety. We aimed to compare antimicrobial resistance phenotypes in Salmonella isolated from poultry samples as influenced by isolation and antimicrobial susceptibility testing methods. Salmonella isolates were cultured from a convenience sample of commercial broiler ceca with and without selective broth enrichment, and resistance phenotypes were determined for 14 antimicrobials using the Sensititre® platform and a qualitative broth breakpoint assay. The broth breakpoint method reported higher resistance to chloramphenicol, sulfisoxazole, and the combination of trimethoprim and sulfamethoxazole, and lower resistance to streptomycin as compared to the Sensititre® assay in trial one. Selective enrichment of samples containing Salmonella in Rappaport-Vassiliadis broth reported lowered detectable resistance to amoxicillin/clavulanic acid, ampicillin, azithromycin, cefoxitin, ceftriaxone, nalidixic acid, and meropenem, and increased resistance to streptomycin and tetracycline than direct-plating samples in trial one. Using matched isolates in trial two, the Sensititre® assay reported higher resistance to chloramphenicol and gentamicin, and lower resistance to nalidixic acid as compared to the broth breakpoint method. These results suggest methodology is a critical consideration in the detection and surveillance of antimicrobial resistance phenotypes in Salmonella isolates from poultry samples and could affect the accuracy of population or industry surveillance insights and intervention strategies.
Rigid Nanoporous Urea-Based Covalent Triazine Frameworks for C2/C1 and CO2/CH4 Gas Separation
Authors: Krishnaraj Chidharth , Jena Himanshu Sekhar , Lecoeuvre Florence , Leus Karen , Van Der Voort Pascal
Published: June 2021

C2/C1 hydrocarbon separation is an important industrial process that relies on energy-intensive cryogenic distillation methods. The use of porous adsorbents to selectively separate these gases is a viable alternative. Highly stable covalent triazine frameworks (urea-CTFs) have been synthesized using 1,3-bis(4-cyanophenyl)urea. Urea-CTFs exhibited gas uptakes of C2H2 (3.86 mmol/g) and C2H4 (2.92 mmol/g) at 273 K and 1 bar and is selective over CH4. Breakthrough simulations show the potential of urea-CTFs for C2/C1 separation.
The Effect of Brewing Process Parameters on Antioxidant Activity and Caffeine Content in Infusions of Roasted and Unroasted Arabica Coffee Beans Originated from Different Countries
Authors: Muzykiewicz-Szymańska Anna , Nowak Anna , Wira Daria , Klimowicz Adam
Published: June 2021

Coffee is one of the most often consumed beverages almost all over the world. The multiplicity of beans, as well as the methods and parameters used to brew, encourages the optimization of the brewing process. The study aimed to analyze the effect of roasting beans, the brewing technique, and its parameters (time and water temperature) on antioxidant activity (determined using several in vitro methods), total polyphenols, flavonoids, and caffeine content. The infusions of unroasted and roasted Arabica beans from Brazil, Colombia, India, Peru, and Rwanda were analyzed. In general, infusions prepared from roasted beans had higher antioxidant activity and the content of above-mentioned compounds. The hot brew method was used to obtain infusions with a higher antioxidant activity, while the cold brew with higher caffeine content. The phenolic compound content in infusions prepared using both techniques depended on the roasting process. Moreover, the bean’s origin, roasting process, and brewing technique had a significant effect on the tested properties, in contrary to brewing time and water temperature (below and above 90 °C), which had less impact. The results confirm the importance of coffee brewing optimization.
The Influence of Different Pretreatment Methods on Color and Pigment Change in Beetroot Products
Authors: Janiszewska-Turak Emilia , Rybak Katarzyna , Grzybowska Ewelina , Konopka Ewelina , Witrowa-Rajchert Dorota
Published: June 2021

Vegetable processing pomace contains valuable substances such as natural colors that can be reused as functional ingredients. Due to a large amount of water, they are an unstable material. The aim of our research was to assess how the pretreatment method (thermal or nonthermal) affects the properties of powders obtained from beet juice and pomace after the freeze-drying process. The raw material was steamed or sonicated for 10 or 15 min, and then squeezed into juice and pomace. Both squeezed products were freeze-dried. The content of dry substance; L*, a*, and b* color parameters; and the content of betalain pigments were analyzed. Pretreatments increased the proportion of red and yellow in the juices. Steam and ultrasound caused a significant reduction in parameter b* in the dried pomace. A significant increase in betanin in lyophilizates was observed after pretreatment with ultrasound and steam for 15 min. As a result of all experiments, dried juices and pomaces can also be used as a colorant source. However, there is higher potential with pomaces due to their additional internal substances as well as better storage properties. After a few hours, juice was sticky and not ready to use.
Activity Guided Isolation of Phenolic Compositions from Anneslea fragrans Wall. and Their Cytoprotective Effect against Hydrogen Peroxide Induced Oxidative Stress in HepG2 Cells
Authors: He Shuyue , Cui Xiaoyan , Khan Afsar , Liu Yaping , Wang Yudan , Cui Qimin , Zhao Tianrui , Cao Jianxin , Cheng Guiguang
Published: June 2021

Anneslea fragrans Wall., commonly known as “Pangpo Tea”, is traditionally used as a folk medicine and healthy tea for the treatment of liver and intestine diseases. The aim of this study was to purify the antioxidative and cytoprotective polyphenols from A. fragrans leaves. After fractionation with polar and nonpolar organic solvents, the fractions of aqueous ethanol extract were evaluated for their total phenolic (TPC) and flavonoid contents (TFC) and antioxidant activities (DPPH, ABTS, and FRAP assays). The n-butanol fraction (BF) showed the highest TPC and TFC with the strongest antioxidant activity. The bio-guided chromatography of BF led to the purification of six flavonoids (1–6) and one benzoquinolethanoid (7). The structures of these compounds were determined by NMR and MS techniques. Compound 6 had the strongest antioxidant capacity, which was followed by 5 and 2. The protective effect of the isolated compounds on hydrogen peroxide (H2O2)-induced oxidative stress in HepG2 cells revealed that the compounds 5 and 6 exhibited better protective effects by inhibiting ROS productions, having no significant difference with vitamin C (p > 0.05), whereas 6 showed the best anti-apoptosis activity. The results suggest that A. fragrans could serve as a valuable antioxidant phytochemical source for developing functional food and health nutraceutical products.
The Neuroprotective Effect of Tea Polyphenols on the Regulation of Intestinal Flora
Authors: Zhang Zhicheng , Zhang Yuting , Li Junmin , Fu Chengxin , Zhang Xin
Published: June 2021

Tea polyphenols (TPs) are the general compounds of natural polyhydroxyphenols extracted in tea. Although a large number of studies have shown that TPs have obvious neuroprotective and neuro repair effects, they are limited due to the low bioavailability in vivo. However, TPs can act indirectly on the central nervous system by affecting the “microflora–gut–brain axis”, in which the microbiota and its composition represent a factor that determines brain health. Bidirectional communication between the intestinal microflora and the brain (microbe–gut–brain axis) occurs through a variety of pathways, including the vagus nerve, immune system, neuroendocrine pathways, and bacteria-derived metabolites. This axis has been shown to influence neurotransmission and behavior, which is usually associated with neuropsychiatric disorders. In this review, we discuss that TPs and their metabolites may provide benefits by restoring the imbalance of intestinal microbiota and that TPs are metabolized by intestinal flora, to provide a new idea for TPs to play a neuroprotective role by regulating intestinal flora.
Bioefficacy of Epaltes divaricata (L.) n-Hexane Extracts and Their Major Metabolites against the Lepidopteran Pests Spodoptera litura (fab.) and Dengue Mosquito Aedes aegypti (Linn.)
Authors: Amala Kesavan , Karthi Sengodan , Ganesan Raja , Radhakrishnan Narayanaswamy , Srinivasan Kumaraswamy , Mostafa Abd El-Zaher M. A. , Al-Ghamdi Abdullah Ahmed , Alkahtani Jawaher , Elshikh Mohamed Soliman , Senthil-Nathan Sengottayan , Vasantha-Srinivasan Prabhakaran , Krutmuang Patcharin
Published: June 2021

The present research investigated the chemical characterization and insecticidal activity of n-Hexane extracts of Epaltes divaricata (NH-EDx) along with their chief derivatives n-Hexadecanoic acid (n-HDa) and n-Octadecanoic acid (n-ODa) against the dengue vector Aedes aegypti and lepidopteran pest Spodoptera litura. Chemical screening of NH-EDx through GC–MS analysis delivered nine major derivatives, and the maximum peak area percentage was observed in n-Hexadecanoic acid (14.63%) followed by n-Octadecadienoic acid (6.73%). The larvicidal activity of NH-EDx (1000 ppm), n-HDa (5 ppm), and n-ODa (5 ppm) against the A. aegypti and S. litura larvae showed significant mortality rate in a dose-dependent way across all the instars. The larvicidal activity was profound in the A. aegypti as compared to the S. litura across all the larval instars. The sublethal dosages of NH-EDx (500 ppm), n-HDa (2.5 ppm), and n-ODa (2.5 ppm) also showed alterations in the larval/pupal durations and adult longevity in both the insect pests. The enzyme activity revealed that the α- and β-carboxylesterase levels were decreased significantly in both the insect pests, whereas the levels of GST and CYP450 uplifted in a dose-dependent manner of NH-EDx, n-HDa, and n-ODa. Correspondingly, midgut tissues such as the epithelial layer (EL), gut lumen (GL), peritrophic matrix (Pm), and brush border membrane (BBM) were significantly altered in their morphology across both A. aegypti and S. litura against the NH-EDx and their bioactive metabolites. NH-EDx and their bioactive metabolites n-HDa and n-ODa showed significant larvicidal, growth retardant, enzyme inhibition, and midgut toxicity effects against two crucial agriculturally and medically challenging insect pest of ecological importance.
Comparison of Surface Properties of Sepiolite and Palygorskite: Surface Energy and Nanoroughness
Authors: Almeida Ricardo , Ferraz Eduardo , Santarén Julio , Gamelas José A. F.
Published: June 2021

The surface properties of two sepiolite samples and one palygorskite sample were compared using inverse gas chromatography (IGC). Samples were previously conditioned at appropriate temperatures for the removal of all zeolitic water. Dispersive (or Lifshitz–van der Waals) component of the surface energy (γsd), specific interactions (−ΔGas) with π electron donor bases (1-alkenes), and nanomorphology indices (IMχT) based on the injections of cycloalkanes and a branched alkane were measured. From IGC data, at 240 °C, it was found that the palygorskite was clearly distinguished from the sepiolites. The palygorskite possessed a lower γsd, larger −ΔGas with 1-alkenes, and remarkably higher IMχT. Slight differences could also be observed between the two sepiolite samples with the same origin. The results were rationalized in terms of the structural features of the two studied minerals. The larger channels of the sepiolite allow for a better insertion of the n-alkanes (longer retention times) while excluding the bulkier probes, such as cyclooctane or 2,2,4-trimethylpentane. Accordingly, the corresponding γsd values were larger and the IMχT values were lower (higher surface nanoroughness) for the sepiolites. Regarding Lewis acid–base properties, all the sample’s surfaces evidenced a very strong amphoteric character. The present results highlight the potential of the evaluated samples for, e.g., adsorption processes with volatile organic compounds or matrix–filler interactions regarding the production of composite structures with Lewis acid–base matrices.
Electrical Interconnection and Bonding by Nano-Locking
Authors: Guo Jielin , Shih Yu-Chou , Shi Frank G.
Published: June 2021

The growing demand for increased chip performance and stable reliability calls for the development of novel off-chip interconnection and bonding methods that can process good electrical, thermal, and mechanical performance simultaneously as well as superior reliability. A chip bonding method with the concept of “nano-locking” (NL) is proposed: the two surfaces are locked together for electrical interconnection, and the connection is stabilized by a dielectric adhesive filled into nanoscale valleys on the interconnecting surfaces. The general applicability of this new method was investigated by applying the method to the die-substrate bonding of two different packages from two different manufacturers. Electrical, optical, and thermal performances as well as reliability tests were carried out. The surface morphology of the bonding package substrates plays an important role in determining the contact resistance at the bonding interfaces. It was shown that samples with different roughness height distribution on the metallic surfaces formed a different total number of contacts and the contact area between the two bonding surfaces under the same bond-line thickness (BLT): a larger number of contact area resulted in a reduced electrical resistance, and thus an improved overall device performance and reliability.
Analysis of Excitability in Resonant Tunneling Diode-Photodetectors
Authors: Zhang Weikang , Al-Khalidi Abdullah , Figueiredo José , Al-Taai Qusay Raghib Ali , Wasige Edward , Hadfield Robert H.
Published: June 2021

We investigate the dynamic behaviour of resonant tunneling diode-photodetectors (RTD-PDs) in which the excitability can be activated by either electrical noise or optical signals. In both cases, we find the characteristics of the stochastic spiking behavior are not only dependent on the biasing positions but also controlled by the intensity of the input perturbations. Additionally, we explore the ability of RTD-PDs to perform optical signal transmission and neuromorphic spike generation simultaneously. These versatile functions indicate the possibility of making use of RTD-PDs for innovative applications, such as optoelectronic neuromorphic circuits for spike-encoded signaling and data processing.
Quantitative Visualization of the Nanomechanical Young’s Modulus of Soft Materials by Atomic Force Microscopy
Authors: Kim Seongoh , Lee Yunkyung , Lee Manhee , An Sangmin , Cho Sang-Joon
Published: June 2021

The accurate measurement of nanoscale mechanical characteristics is crucial in the emerging field of soft condensed matter for industrial applications. An atomic force microscope (AFM) can be used to conduct nanoscale evaluation of the Young’s modulus on the target surface based on site-specific force spectroscopy. However, there is still a lack of well-organized study about the nanomechanical interpretation model dependence along with cantilever stiffness and radius of the tip apex for the Young’s modulus measurement on the soft materials. Here, we present the fast and accurate measurement of the Young’s modulus of a sample’s entire scan surface using the AFM in a newly developed PinPointTM nanomechanical mode. This approach enables simultaneous measurements of topographical data and force–distance data at each pixel within the scan area, from which quantitative visualization of the pixel-by-pixel topographical height and Young’s modulus of the entire scan surface was realized. We examined several models of contact mechanics and showed that cantilevers with proper mechanical characteristics such as stiffness and tip radius can be used with the PinPointTM mode to accurately evaluate the Young’s modulus depending on the sample type.
Electronic Structure and d-Band Center Control Engineering over Ni-Doped CoP3 Nanowall Arrays for Boosting Hydrogen Production
Authors: Qi Jing , Wu Tianli , Xu Mengyao , Zhou Dan , Xiao Zhubing
Published: June 2021

To address the challenge of highly efficient water splitting into H2, successful fabrication of novel porous three-dimensional Ni-doped CoP3 nanowall arrays on carbon cloth was realized, resulting in an effective self-supported electrode for the electrocatalytic hydrogen-evolution reaction. The synthesized samples exhibit rough, curly, and porous structures, which are beneficial for gaseous transfer and diffusion during the electrocatalytic process. As expected, the obtained Ni-doped CoP3 nanowall arrays with a doping concentration of 7% exhibit the promoted electrocatalytic activity. The achieved overpotentials of 176 mV for the hydrogen-evolution reaction afford a current density of 100 mA cm−2, which indicates that electrocatalytic performance can be dramatically enhanced via Ni doping. The Ni-doped CoP3 electrocatalysts with increasing catalytic activity should have significant potential in the field of water splitting into H2. This study also opens an avenue for further enhancement of electrocatalytic performance through tuning of electronic structure and d-band center by doping.
Growth and Characterisation Studies of Eu3O4 Thin Films Grown on Si/SiO2 and Graphene
Authors: Aboljadayel Razan O. M. , Ionescu Adrian , Burton Oliver J. , Cheglakov Gleb , Hofmann Stephan , Barnes Crispin H. W.
Published: June 2021

We report the growth, structural and magnetic properties of the less studied Eu-oxide phase, Eu3O4, thin films grown on a Si/SiO2 substrate and Si/SiO2/graphene using molecular beam epitaxy. The X-ray diffraction scans show that highly textured crystalline Eu3O4(001) films are grown on both substrates, whereas the film deposited on graphene has a better crystallinity than that grown on the Si/SiO2 substrate. The SQUID measurements show that both films have a Curie temperature of ∼5.5±0.1 K, with a magnetic moment of ∼320 emu/cm3 at 2 K. The mixed valence of the Eu cations has been confirmed by the qualitative analysis of the depth-profile X-ray photoelectron spectroscopy measurements with the Eu2+:Eu3+ ratio of 28:72. However, surprisingly, our films show no metamagnetic behaviour as reported for the bulk and powder form. Furthermore, the microscopic optical images and Raman measurements show that the graphene underlayer remains largely intact after the growth of the Eu3O4 thin films.
Tolerability and Safety of a Novel Ketogenic Ester, Bis-Hexanoyl (R)-1,3-Butanediol: A Randomized Controlled Trial in Healthy Adults
Authors: Chen Oliver , Blonquist Traci , Mah Eunice , Sanoshy Kristen , Beckman Dawn , Nieman Kristin , Winters Barbara , Anthony Joshua , Verdin Eric , Newman John , Stubbs Brianna
Published: June 2021

Nutritional ketosis is a state of mildly elevated blood ketone concentrations resulting from dietary changes (e.g., fasting or reduced carbohydrate intake) or exogenous ketone consumption. In this study, we determined the tolerability and safety of a novel exogenous ketone diester, bis-hexanoyl-(R)-1,3-butanediol (BH-BD), in a 28-day, randomized, double-blind, placebo-controlled, parallel trial (NCT04707989). Healthy adults (n = 59, mean (SD), age: 42.8 (13.4) y, body mass index: 27.8 (3.9) kg/m2) were randomized to consume a beverage containing 12.5 g (Days 0–7) and 25 g (Days 7–28) of BH-BD or a taste-matched placebo daily with breakfast. Tolerability, stimulation, and sedation were assessed daily by standardized questionnaires, and blood and urine samples were collected at Days 0, 7, 14, and 28 for safety assessment. There were no differences in at-home composite systemic and gastrointestinal tolerability scores between BH-BD and placebo at any time in the study, or in acute tolerability measured 1-h post-consumption in-clinic. Weekly at-home composite tolerability scores did not change when BH-BD servings were doubled. At-home scores for stimulation and sedation did not differ between groups. BH-BD significantly increased blood ketone concentrations 1-h post-consumption. No clinically meaningful changes in safety measures including vital signs and clinical laboratory measurements were detected within or between groups. These results support the overall tolerability and safety of consumption of up to 25 g/day BH-BD.
Nutrition and Autism Spectrum Disorder: Between False Myths and Real Research-Based Opportunities
Authors: Narzisi Antonio , Masi Gabriele , Grossi Enzo
Published: June 2021

Autism Spectrum Disorder (ASD) is a multicomplex disorder characterized by an umbrella of specific issues in the areas of social communication, restricted interests, and repetitive behaviors [...]
Maternal Folic Acid Intake and Methylation Status of Genes Associated with Ventricular Septal Defects in Children: Case–Control Study
Authors: González-Peña Sandra M. , Calvo-Anguiano Geovana , Martínez-de-Villarreal Laura E. , Ancer-Rodríguez Patricia R. , Lugo-Trampe José J. , Saldivar-Rodríguez Donato , Hernández-Almaguer María D. , Calzada-Dávila Melissa , Guerrero-Orjuela Ligia S. , Campos-Acevedo Luis D.
Published: June 2021

Background: DNA methylation is the best epigenetic mechanism for explaining the interactions between nutrients and genes involved in intrauterine growth and development programming. A possible contributor of methylation abnormalities to congenital heart disease is the folate methylation regulatory pathway; however, the mechanisms and methylation patterns of VSD-associated genes are not fully understood. Objective: To determine if maternal dietary intake of folic acid (FA) is related to the methylation status (MS) of VSD-associated genes (AXIN1, MTHFR, TBX1, and TBX20). Methods: Prospective case–control study; 48 mothers and their children were evaluated. The mothers’ dietary variables were collected through a food frequency questionnaire focusing on FA and the consumption of supplements with FA. The MS of promoters of genes was determined in the children. Results: The intake of FA supplements was significantly higher in the control mothers. In terms of maternal folic acid consumption, significant differences were found in the first trimester of pregnancy. Significant differences were observed in the MS of MTHFR and AXIN1 genes in VSD and control children. A correlation between maternal FA supplementation and MS of AXIN1 and TBX20 genes was found in control and VSD children, respectively. Conclusions: A lower MS of AXIN1 genes and a higher MS of TBX20 genes is associated with FA maternal supplementation.
Patient Perspectives of Living with Coeliac Disease and Accessing Dietetic Services in Rural Australia: A Qualitative Study
Authors: Lee Rachelle , Crowley Elesa T. , Baines Surinder K. , Heaney Susan , Brown Leanne J.
Published: June 2021

Adapting to living with coeliac disease requires individuals to learn about and follow a strict gluten-free diet. Utilising a qualitative inductive approach, this study aimed to explore the perspectives of adults diagnosed with coeliac disease who have accessed dietetic services in a rural outpatient setting. A purposive sample of adults with coeliac disease who had accessed dietetic services from two rural dietetic outpatient clinics were recruited. Semi-structured interviews were conducted by telephone. Data were thematically analysed. Six participants were recruited and interviewed. Three key themes emerged: (i) optimising individualised support and services, (ii) adapting to a gluten-free diet in a rural context, and (iii) managing a gluten-free diet within the context of interpersonal relationships. Key issues identified in the rural context were access to specialist services and the increased cost of gluten-free food in more remote areas. The findings of this study have highlighted the difficulties associated with coeliac disease management and how dietetic consultation has the potential to influence confidence in management and improve lifestyle outcomes. Further qualitative research is required to expand on the findings of this study and inform future dietetic practice that meets the expectations and individual needs of people with coeliac disease in rural settings.
Nutritional Significance of Fruit and Fruit Products in the Average Polish Diet
Authors: Rejman Krystyna , Górska-Warsewicz Hanna , Kaczorowska Joanna , Laskowski Wacław
Published: June 2021

The aim of the present study was to analyze the supply of energy, 19 nutrients, free sugars, and dietary fiber in the average Polish diet from fruit and fruit products. Our analysis is based on 2016 data from the national representative household budget survey conducted on a sample of 36,886 households, yielding a population of 99,230 individuals. Fruit and fruit products provided 3.12% of energy to the average diet in Poland with the highest share of bananas and apples. The highest significance of this food group was found for vitamin C (23.65%), including citrus fruits providing 8.03% of vitamin C, berries (5.97%), other fruits (3.45%), and apples (3.13%). The share of fruit and their products in the supply of free sugars is equally high and amounts to 23.52%. This means that apples provide 6.34% of free sugars, while other fruits also have a high supply of free sugars, including berries 3.68%, stone fruits 3.06%, bananas 2.56%, and citrus fruits 2.54%. The proportion of supply exceeding the percentage of energy (which was 3.12%) was obtained for carbohydrate (5.79%), and fiber (13.66%). The food group studied was particularly important (more than 5% share) in providing four minerals: potassium (8.59%), iron (5.07%), magnesium (5.51%), copper (8.81%), and three vitamins: vitamin C (23.65%), vitamin B6 (5.74%), and vitamin E (5.53%). The influence of sociodemographic and economic characteristics of households on the structure of energy and nutrient supply from fruit and fruit products was assessed using cluster analysis. There were four clusters characterized by different energy, nutrient, and fiber supply. The factors with the highest statistical significance on the supply of energy, nutrients, and fiber from fruit and fruit products were month of study, income, degree of urbanization, education, size of town, and land use. The obtained results concerning energy and nutrient supply from fruits and fruit products are important for the Polish society from the public health point of view, as indicated in the discussion of results and conclusions.
Breakfast Consumption Habits at Age 6 and Cognitive Ability at Age 12: A Longitudinal Cohort Study
Authors: Liu Jianghong , Wu Lezhou , Um Phoebe , Wang Jessica , Kral Tanja V. E. , Hanlon Alexandra , Shi Zumin
Published: June 2021

This study aimed to assess the relationship between breakfast composition and long-term regular breakfast consumption and cognitive function. Participants included 835 children from the China Jintan Cohort Study for the cross-sectional study and 511 children for the longitudinal study. Breakfast consumption was assessed at ages 6 and 12 through parental and self-administered questionnaires. Cognitive ability was measured as a composition of IQ at age 6 and 12 and academic achievement at age 12, which were assessed by the Chinese versions of the Wechsler Intelligence Scales and standardized school reports, respectively. Multivariable general linear and mixed models were used to evaluate the relationships between breakfast consumption, breakfast composition and cognitive performance. In the longitudinal analyses, 94.7% of participants consumed breakfast ≥ 4 days per week. Controlling for nine covariates, multivariate mixed models reported that compared to infrequent breakfast consumption, regular breakfast intake was associated with an increase of 5.54 points for verbal and 4.35 points for full IQ scores (p < 0.05). In our cross-sectional analyses at age 12, consuming grain/rice or meat/egg 6–7 days per week was significantly associated with higher verbal, performance, and full-scale IQs, by 3.56, 3.69, and 4.56 points, respectively (p < 0.05), compared with consuming grain/rice 0–2 days per week. Regular meat/egg consumption appeared to facilitate academic achievement (mean difference = 0.232, p = 0.043). No association was found between fruit/vegetable and dairy consumption and cognitive ability. In this 6-year longitudinal study, regular breakfast habits are associated with higher IQ. Frequent grain/rice and meat/egg consumption during breakfast may be linked with improved cognitive function in youth.
Investigation of Long COVID Prevalence and Its Relationship to Epstein-Barr Virus Reactivation
Authors: Gold Jeffrey E. , Okyay Ramazan A. , Licht Warren E. , Hurley David J.
Published: June 2021

Coronavirus disease 2019 (COVID-19) patients sometimes experience long-term symptoms following resolution of acute disease, including fatigue, brain fog, and rashes. Collectively these have become known as long COVID. Our aim was to first determine long COVID prevalence in 185 randomly surveyed COVID-19 patients and, subsequently, to determine if there was an association between occurrence of long COVID symptoms and reactivation of Epstein–Barr virus (EBV) in 68 COVID-19 patients recruited from those surveyed. We found the prevalence of long COVID symptoms to be 30.3% (56/185), which included 4 initially asymptomatic COVID-19 patients who later developed long COVID symptoms. Next, we found that 66.7% (20/30) of long COVID subjects versus 10% (2/20) of control subjects in our primary study group were positive for EBV reactivation based on positive titers for EBV early antigen-diffuse (EA-D) IgG or EBV viral capsid antigen (VCA) IgM. The difference was significant (p < 0.001, Fisher’s exact test). A similar ratio was observed in a secondary group of 18 subjects 21–90 days after testing positive for COVID-19, indicating reactivation may occur soon after or concurrently with COVID-19 infection. These findings suggest that many long COVID symptoms may not be a direct result of the SARS-CoV-2 virus but may be the result of COVID-19 inflammation-induced EBV reactivation.
Repurposing Drugs to Treat Heart and Brain Illness
Authors: Cantrell Maranda S. , Soto-Avellaneda Alejandro , Wall Jackson D. , Ajeti Aaron D. , Morrison Brad E. , Warner Lisa R. , McDougal Owen M.
Published: June 2021

Drug development is a complicated, slow and expensive process with high failure rates. One strategy to mitigate these factors is to recycle existing drugs with viable safety profiles and have gained Food and Drug Administration approval following extensive clinical trials. Cardiovascular and neurodegenerative diseases are difficult to treat, and there exist few effective therapeutics, necessitating the development of new, more efficacious drugs. Recent scientific studies have led to a mechanistic understanding of heart and brain disease progression, which has led researchers to assess myriad drugs for their potential as pharmacological treatments for these ailments. The focus of this review is to survey strategies for the selection of drug repurposing candidates and provide representative case studies where drug repurposing strategies were used to discover therapeutics for cardiovascular and neurodegenerative diseases, with a focus on anti-inflammatory processes where new drug alternatives are needed.
Pharmacokinetics in Zebrafish Embryos (ZFE) Following Immersion and Intrayolk Administration: A Fluorescence-Based Analysis
Authors: Guarin Marlly , Ny Annelii , De Croze Noémie , Maes Jan , Léonard Marc , Annaert Pieter , de Witte Peter
Published: June 2021

Zebrafish embryos (ZFE) have increasingly gained in popularity as a model to perform safety screenings of compounds. Although immersion of ZFE is the main route of exposure used, evidence shows that not all small molecules are equally absorbed, possibly resulting in false-negative readouts and incorrect conclusions. In this study, we compared the pharmacokinetics of seven fluorescent compounds with known physicochemical properties that were administered to two-cell stage embryos by immersion or by IY microinjection. Absorption and distribution of the dyes were followed at various timepoints up to 120 hpf by spatiotemporal fluorescence imaging. The concentration (10 µM) and dose (2 mg/kg) used were selected as quantities typically applied in preclinical experiments and zebrafish studies. The data show that in the case of a lipophilic compound (log D: 1.73) the immersion procedure resulted in an intrabody exposure which is similar or higher than that seen after the IY microinjection. In contrast, zero to low intrabody exposure was reached after immersion of the embryos with less lipophilic compounds. In the latter case IY microinjection, a technical procedure that can be easily automated, is highly recommended.
Capillary Liquid Chromatography for the Determination of Terpenes in Botanical Dietary Supplements
Authors: Ponce-Rodríguez Henry , Verdú-Andrés Jorge , Campíns-Falcó Pilar , Herráez-Hernández Rosa
Published: June 2021

Dietary supplements of botanical origin are increasingly consumed due to their content of plant constituents with potential benefits on health and wellness. Among those constituents, terpenes are gaining attention because of their diverse biological activities (anti-inflammatory, antibacterial, geroprotective, and others). While most of the existing analytical methods have focused on establishing the terpenic fingerprint of some plants, typically by gas chromatography, methods capable of quantifying representative terpenes in herbal preparations and dietary supplements with combined high sensitivity and precision, simplicity, and high throughput are still necessary. In this study, we have explored the utility of capillary liquid chromatography (CapLC) with diode array detection (DAD) for the determination of different terpenes, namely limonene, linalool, farnesene, α-pinene, and myrcene. An innovative method is proposed that can be applied to quantify the targets at concentration levels as low as 0.006 mg per gram of sample with satisfactory precision, and a total analysis time <30 min per sample. The reliability of the proposed method has been tested by analyzing different dietary supplements of botanical origin, namely three green coffee extract-based products, two fat burnings containing Citrus aurantium (bitter orange), and an herbal preparation containing lime and leaves of orange trees.