bluuebunny/crossref_metadata_2025 huggingface dataset:
Green Synthesis, Characterization and Application of Silver Nanoparticles Using Bioflocculant: A Review
Authors: Nkosi Nkanyiso C. , Basson Albertus K. , Ntombela Zuzingcebo G. , Dlamini Nkosinathi G. , Pullabhotla Rajasekhar V. S. R.
Published: May 2024

Nanotechnology has emerged as an effective means of removing contaminants from water. Traditional techniques for producing nanoparticles, such as physical methods (condensation and evaporation) and chemical methods (oxidation and reduction), have demonstrated high efficiency. However, these methods come with certain drawbacks, including the significant energy requirement and the use of costly and hazardous chemicals that may cause nanoparticles to adhere to surfaces. To address these limitations, researchers are actively developing alternative procedures that are cost-effective, environmentally safe, and user-friendly. One promising approach involves biological synthesis, which utilizes plants or microorganisms as reducing and capping agents. This review discusses various methods of nanoparticle synthesis, with a focus on biological synthesis using naturally occurring bioflocculants from microorganisms. Bioflocculants offer several advantages, including harmlessness, biodegradability, and minimal secondary pollution. Furthermore, the review covers the characterization of synthesized nanoparticles, their antimicrobial activity, and cytotoxicity. Additionally, it explores the utilization of these NPs in water purification and dye removal processes.
Unveiling the Potential of JAK Inhibitors in Inflammatory Bowel Disease
Authors: Kamal Shahed , Lo Sheng Wei , McCall Samantha , Rodrigues Beverly , Tsoi Andrew H. , Segal Jonathan P.
Published: May 2024

Background: Janus kinase (JAK) inhibitors represent a novel class of oral therapies showing efficacy in treating ulcerative colitis (UC) and Crohn’s disease (CD), challenging conventional treatment paradigms. Summary: This review provides an overview of the potential novel uses of JAK inhibitors, focusing on their current approved indications and exploring possibilities beyond these indications. Tofacitinib and filgotinib are approved for UC, while upadacitinib is approved for both UC and CD. Additionally, their potential in acute severe UC, as steroid alternatives, and in managing fistulizing CD or extraintestinal manifestations are discussed. Key Message: JAK inhibitors play an important role in IBD (inflammatory bowel disease) treatment; however, clinicians must balance their promising efficacy with safety concerns. Individualized care and vigilance are essential for optimizing therapeutic benefits while mitigating potential adverse effects. Further research is necessary to clarify their efficacy, safety, and potential applications.
Influence of Cluster-Situated Regulator PteF in Filipin Biosynthetic Cluster on Avermectin Biosynthesis in Streptomyces avermitilis
Authors: Du Guozhong , Yang Xue , Wu Zhengxiong , Pan Minghui , Dong Zhuoxu , Zhang Yanyan , Xiang Wensheng , Li Shanshan
Published: May 2024

Crosstalk regulation is widespread in Streptomyces species. Elucidating the influence of a specific regulator on target biosynthetic gene clusters (BGCs) and cell metabolism is crucial for strain improvement through regulatory protein engineering. PteF and PteR are two regulators that control the biosynthesis of filipin, which competes for building blocks with avermectins in Streptomyces avermitilis. However, little is known about the effects of PteF and PteR on avermectin biosynthesis. In this study, we investigated their impact on avermectin biosynthesis and global cell metabolism. The deletion of pteF resulted in a 55.49% avermectin titer improvement, which was 23.08% higher than that observed from pteR deletion, suggesting that PteF plays a more significant role in regulating avermectin biosynthesis, while PteF hardly influences the transcription level of genes in avermectin and other polyketide BGCs. Transcriptome data revealed that PteF exhibited a global regulatory effect. Avermectin production enhancement could be attributed to the repression of the tricarboxylic acid cycle and fatty acid biosynthetic pathway, as well as the enhancement of pathways supplying acyl-CoA precursors. These findings provide new insights into the role of PteF on avermectin biosynthesis and cell metabolism, offering important clues for designing and building efficient metabolic pathways to develop high-yield avermectin-producing strains.
1,25-Dihydroxyvitamin D3 Suppresses Prognostic Survival Biomarkers Associated with Cell Cycle and Actin Organization in a Non-Malignant African American Prostate Cell Line
Authors: Johnson Jabril R. , Martini Rachel N. , Yuan Yate-Ching , Woods-Burnham Leanne , Walker Mya , Ortiz-Hernandez Greisha L. , Kobeissy Firas , Galloway Dorothy , Gaddy Amani , Oguejiofor Chidinma , Allen Blake , Lewis Deyana , Davis Melissa B. , Kimbro K. Sean , Yates Clayton C. , Murphy Adam B. , Kittles Rick A.
Published: May 2024

Vitamin D3 is a steroid hormone that confers anti-tumorigenic properties in prostate cells. Serum vitamin D3 deficiency has been associated with advanced prostate cancer (PCa), particularly affecting African American (AA) men. Therefore, elucidating the pleiotropic effects of vitamin D on signaling pathways, essential to maintaining non-malignancy, may provide additional drug targets to mitigate disparate outcomes for men with PCa, especially AA men. We conducted RNA sequencing on an AA non-malignant prostate cell line, RC-77N/E, comparing untreated cells to those treated with 10 nM of vitamin D3 metabolite, 1α,25(OH)2D3, at 24 h. Differential gene expression analysis revealed 1601 significant genes affected by 1α,25(OH)2D3 treatment. Pathway enrichment analysis predicted 1α,25(OH)2D3- mediated repression of prostate cancer, cell proliferation, actin cytoskeletal, and actin-related signaling pathways (p < 0.05). Prioritizing genes with vitamin D response elements and associating expression levels with overall survival (OS) in The Cancer Genome Atlas Prostate Adenocarcinoma (TCGA PRAD) cohort, we identified ANLN (Anillin) and ECT2 (Epithelial Cell Transforming 2) as potential prognostic PCa biomarkers. Both genes were strongly correlated and significantly downregulated by 1α,25(OH)2D3 treatment, where low expression was statistically associated with better overall survival outcomes in the TCGA PRAD public cohort. Increased ANLN and ECT2 mRNA gene expression was significantly associated with PCa, and Gleason scores using both the TCGA cohort (p < 0.05) and an AA non-malignant/tumor-matched cohort. Our findings suggest 1α,25(OH)2D3 regulation of these biomarkers may be significant for PCa prevention. In addition, 1α,25(OH)2D3 could be used as an adjuvant treatment targeting actin cytoskeleton signaling and actin cytoskeleton-related signaling pathways, particularly among AA men.
Estetrol Inhibits Endometriosis Development in an In Vivo Murine Model
Authors: Zabala Ana Sofia , Conforti Rocío Ayelem , Delsouc María Belén , Filippa Verónica , Montt-Guevara Maria Magdalena , Giannini Andrea , Simoncini Tommaso , Vallcaneras Sandra Silvina , Casais Marilina
Published: May 2024

Endometriosis is characterized by the growth of endometrial-like tissue outside the uterus, and it is associated with alterations in the expression of hormone receptors and inflammation. Estetrol (E4) is a weak estrogen that recently has been approved for contraception. We evaluated the effect of E4 on the growth of endometriotic-like lesions and the expression of TNF-α, estrogen receptors (ERs), and progesterone receptors (PRs) in an in vivo murine model. Endometriosis was induced surgically in female C57BL/6 mice. E4 was delivered via Alzet pump (3 mg/kg/day) from the 15th postoperative day for 4 weeks. E4 significantly reduced the volume (p < 0.001) and weight (p < 0.05) of ectopic lesions. Histologically, E4 did not affect cell proliferation (PCNA immunohistochemistry) but it did increase cell apoptosis (TUNEL assay) (p < 0.05). Furthermore, it modulated oxidative stress (SOD, CAT, and GPX activity, p < 0.05) and increased lipid peroxidation (TBARS/MDA, p < 0.01). Molecular analysis showed mRNA (RT-qPCR) and protein (ELISA) expression of TNF-α decreased (p < 0.05) and mRNA expression of Esr2 reduced (p < 0.05), in contrast with the increased expression of Esr1 (p < 0.01) and Pgr (p < 0.05). The present study demonstrates for the first time that E4 limited the development and progression of endometriosis in vivo.
Comparative Analysis of Posiphen Pharmacokinetics across Different Species—Similar Absorption and Metabolism in Mouse, Rat, Dog and Human
Authors: Maccecchini Maria L. , Mould Diane R.
Published: May 2024

Posiphen is a small molecule that exhibits neuroprotective properties by targeting multiple neurotoxic proteins involved in axonal transport, synaptic transmission, neuroinflammation, and cell death. Its broad-spectrum effects make it a promising candidate for treating neurodegenerative conditions, including Alzheimer’s and Parkinson’s diseases. Despite extensive investigation with animal models and human subjects, a comprehensive comparative analysis of Posiphen’s pharmacokinetics across studies remains elusive. Here, we address this gap by examining the metabolic profiles of Posiphen and its breakdown into two primary metabolites—N1 and N8—across species by measuring their concentrations in plasma, brain, and CSF using the LC-MS/MS method. While all three compounds effectively inhibit neurotoxic proteins, the N1 metabolite is associated with adverse effects. Our findings reveal the species-specific behavior of Posiphen, with both Posiphen and N8 being predominant in various species, while N1 remains a minor constituent, supporting the drug’s safety. Moreover, in plasma, Posiphen consistently showed fast clearance of all metabolites within 8 h in animal models and in human subjects, whereas in CSF or brain, the compound has an extended half-life of over 12 h. Combining all our human data and analyzing them by population pharmacokinetics showed that there are no differences between healthy volunteers, Alzheimer’s, and Parkinson’s patients. It also showed that Posiphen is absorbed and metabolized in a similar fashion across all animal species and human groups tested. These observations have critical implications for understanding the drug’s safety, therapeutic effect, and clinical translation.
Nerve Bundle Density and Expression of NGF and IL-1β Are Intra-Individually Heterogenous in Subtypes of Endometriosis
Authors: Sreya Mahfuza , Tucker Dwayne R. , Yi Jennifer , Alotaibi Fahad T. , Lee Anna F. , Noga Heather , Yong Paul J.
Published: May 2024

Endometriosis is a gynecological disorder associated with local inflammation and neuroproliferation. Increased nerve bundle density has been attributed to increased expression of nerve growth factor (NGF) and interleukin–1β (IL-1β). Immunohistochemical analysis was carried out on 12 patients presenting with all three anatomic subtypes of endometriosis (deep, superficial peritoneal, endometrioma) at surgery, with at least two surgically excised subtypes available for analysis. Immunolocalization for nerve bundle density around endometriosis using protein gene product 9.5 (PGP9.5), as well as NGF and IL-1β histoscores in endometriosis epithelium/stroma, was performed to evaluate differences in scores between lesions and anatomic subtypes per patient. Intra-individual heterogeneity in scores across lesions was assessed using the coefficient of variation (CV). The degree of score variability between subtypes was evaluated using the percentage difference between mean scores from one subtype to another subtype for each marker. PGP9.5 nerve bundle density was heterogenous across multiple subtypes of endometriosis, ranging from 50.0% to 173.2%, where most patients (8/12) showed CV ≥ 100%. The percentage difference in scores showed that PGP9.5 nerve bundle density and NGF and IL-1β expression were heterogenous between anatomic subtypes within the same patient. Based on these observations of intra-individual heterogeneity, we conclude that markers of neuroproliferation in endometriosis should be stratified by anatomic subtype in future studies of clinical correlation.
Review of Laboratory Testing and Biomarker Screening for Preeclampsia
Authors: Oladipo Antonia F. , Jayade Maansi
Published: May 2024

The purpose of this review is to elucidate the different laboratory and biomarker testing methods available for screening and diagnosis of preeclampsia. These include routine testing, such as blood pressure readings, qualitative and quantitative urine testing, complete blood count with platelets, serum creatinine levels, liver chemistries, and serum bilirubin levels. This review also details the use of non-routine testing, such as screening for angiogenic and anti-angiogenic markers, such as placental growth factor (PlGF) and soluble fms-like tyrosine kinase-1 (sFlt-1). Blood pressure measurements and proteinuria are the most routinely used screening tools used for preeclampsia and there are limited data on the utility of other screening techniques because of a greater focus on the etiology and treatment of preeclampsia. Similarly, serum angiogenic biomarkers are not routinely collected, so there is limited evidence regarding using them as screening tools for preeclampsia and more data are needed to determine their significance in the screening and diagnosis of preeclampsia.
Differential Scanning Calorimetry of Proteins and the Two-State Model: Comparison of Two Formulas
Authors: Yeritsyan Knarik , Badasyan Artem
Published: May 2024

Differential Scanning Calorimetry (DSC) is a regular and powerful tool to measure the specific heat profile of various materials. In order to connect the measured profile to the properties of a particular protein, a model is required to fit. We discuss here the application of an exact two-state formula with its approximation and process the DSC experimental data on protein folding in water. The approximate formula relies on the smallness of the transition interval, which is different for each protein. With an example of the set of 33 different proteins, we show the practical validity of the approximation and the equivalence of exact and approximate two-state formulas for processing DSC data.
Analysis of Multi-Stakeholder Behavioral Strategies in the Construction and Demolition Waste Recycling Industry through an Evolutionary Game Theory
Authors: Wang Yanyan , Qi Lijun , Cui Wenjing
Published: May 2024

Construction and demolition waste (C&DW) recycling products have drawn worldwide attention over the past few decades. There is a general agreement among researchers that C&DW recycling is an important means for curbing the deterioration of the environment. Previous papers mainly focused on the decision-making behavior of dual stakeholders or tripartite stakeholders, as well as the lack of cooperation among multiple stakeholders. This study explored a dynamic evolutionary game model with three different parameter conditions to research the decision-making behaviors and stable strategies of the multi-stakeholders involved in the C&DW recycling product industry, including government departments and some enterprises. This research also investigated how the government’s supervision costs, penalties applied to enterprises, and resource taxes affect the dynamic evolution process of C&DW recycling. This research conducted numerical simulations using Python to analyze stakeholders’ behavioral evolutionarily stable strategy (ESS) and the sensitivity to main factors in each stage of the C&DW recycling process to accelerate the development of construction waste resource utilization. Based on the results of the evolutionary game, this paper proposed that the roles of multi-stakeholders are different at different stages of industry development, and that supervision costs, penalties, and resource tax have their own impacts on the C&DW recycling product industry. The paper suggests a range of discussions and simulation studies to highlight the significance of the government’s refined and moderate adjustments to the regulatory incentive system and the level of government regulation and involvement at different stages of the process. These adjustments are aimed at promoting the sustainable recycling and utilization of construction and demolition waste (C&DW) products within some countries’ construction industry.
Expanded Graphite (EG) Stabilization of Stearic and Palmitic Acid Mixture for Thermal Management of Photovoltaic Cells
Authors: Sacchet Sereno , Valentini Francesco , Benin Alice , Guidolin Marco , Po Riccardo , Fambri Luca
Published: May 2024

In this work, passive cooling systems for the revamping of existent silicon photovoltaic (PV) cells were developed and analysed in order to mitigate the efficiency loss caused by temperature rise in the hot season. For this purpose, expanded graphite (EG) was used to stabilize a phase change material (PCM) with a melting temperature close to 53 °C in order to realize thermal management systems (TMSs) able to store heat at constant temperature during melting and releasing it in crystallization. In particular, stearic and palmitic acid mixture (PA-SA) was shape-stabilized in EG at different concentrations (10, 12 and 14 part per hundred ratio) under vacuum into a rotary evaporation apparatus followed by cold compaction; PA-SA leakage was reduced due to its intercalation between the graphite lamellae, and the thermal conductivity necessary to maximize the heat transfer to a bulk TMS was improved via powder cold compaction, which minimizes voids and creates preferential thermal conductive patterns. The composite materials, stable till 150 °C, were tested by differential scanning calorimetry (DSC) at 1 °C/min to precisely determine the phase transition temperatures and the enthalpic content, which was only slightly reduced from 196 J/g of the neat PCM to 169 J/g due to the very low EG fraction necessary for the stabilization. Despite only the 14:100 EG-to-PA-SA ratio, the system’s thermal conductivity was enhanced 40 times with respect to the neat PCM (from 0.2 to 8.3 W/(m K), value never reached in works present in the literature), with a good convergence of the values evaluated through hot disk tests and laser flash analysis (LFA), finding correlation with both graphitic content and density. In order to completely avoid leaking with the consequent dispersion of PCM in the environment during the final application, all the samples were encapsulated in a PE-made film. The mechanical properties were evaluated with compression tests at 30 °C and 80 °C simulating a possible compressive stress deriving from the contact needed to maintain the TMS position on the rear of the PV cells. Finally, the material response was simulated by imposing thermal cycles into a climatic chamber and reproducing the three hottest and coldest days of summer 2022 of two Italian locations, Verona (Veneto, 45° N, 11° E) and Gela (Sicily, 37° N, 14° E), thus highlighting the thermal management effects with delays in temperature increase and daily peak temperature smoothing. The role of EG is strategic for the processing and the properties of the resulting composites in order to realize a proper compromise between the melting enthalpy of PCM and the thermal conductivity enhancement given by EG.
Presepsin Levels in Pediatric Patients with Fever and Suspected Sepsis: A Pilot Study in an Emergency Department
Authors: Gatto Antonio , Mantani Lucia , Gola Caterina , Pansini Valeria , Di Sarno Lorenzo , Capossela Lavinia , Ferretti Serena , Graglia Benedetta , Chiaretti Antonio
Published: May 2024

Sepsis is a life-threatening condition that affects 1.2 million children annually. Although there are several criteria for diagnosing this condition, signs are often nonspecific, and identifying sepsis is challenging. In this context, presepsin (P-SEP) seems to be a promising new biomarker since its plasma levels increase earlier than other sepsis-related proteins and its measurement is faster. We enrolled 157 minors who presented to the Pediatric Emergency Department of Agostino Gemelli Hospital with fever and suspected sepsis. Biochemical, anamnestic, and clinical data were collected. Viral agents were identified as the causative factor in 64 patients, who had an average P-SEP value of 309.04 pg/mL (SD ± 273.2), versus an average P-SEP value of 526.09 pg/mL (SD ± 657) found in 27 bacterial cases (p value: 0.0398). Four cases of overt sepsis had an average P-SEP value of 3328.5 pg/mL (SD ± 1586.6). The difference in P-SEP levels in viral versus bacterial infections was found to be statistically significant; therefore, P-SEP may have a central role in the evaluation of febrile children, helping clinicians distinguish between these two etiologies. Furthermore, amongst the cases of confirmed sepsis, P-SEP was always greater than 2000 pg/mL, while C-reactive protein and procalcitonin values appeared lower than what was considered significant.
Colorants: Moving to the Next Stage
Authors: Harriman Anthony
Published: May 2024

As Editor-in-Chief of Colorants [ISSN: 2079-6447], I have watched the progressive growth of this journal and witnessed the expansion of topics way beyond the initial consideration [...]
COVID-19 Therapeutics Use by Social Deprivation Index in England, July 2020–April 2023
Authors: Falola Angela , Squire Hanna , Bou-Antoun Sabine , Løchen Alessandra , Brown Colin S. , Demirjian Alicia
Published: May 2024

Coronavirus disease-19 (COVID-19) has disproportionately affected certain demographics in England, exacerbating existing health disparities. Effective therapeutics are a critical line of defence against COVID-19, particularly for patients at elevated risk for severe disease. Surveillance systems were established to monitor the usage of COVID-19 therapeutics in hospital and community settings and to inform stewardship. Three antiviral therapies—nirmatrelvir plus ritonavir (Paxlovid®), remdesivir (Veklury®), and molnupiravir (Lagevrio®)—and two neutralising monoclonal antibody therapies (nMAbs)—sotrovimab (Xevudy®) and casirivimab with imdevimab (Ronapreve®)—were in use in England between July 2020 and April 2023. This paper aims to illuminate trends in the utilisation of COVID-19 therapeutics treatment in both hospital and community settings, stratified by the Index of Multiple Deprivation (IMD) in England. Chapter 3 of the English Surveillance Programme for Antimicrobial Utilisation and Resistance (ESPAUR) Report 2022 to 2023 also discusses the epidemiological surveillance of these five directly acting antiviral COVID-19 therapeutics’ use in England between 2022 and 2023.
EEG and Physiological Signals Dataset from Participants during Traditional and Partially Immersive Learning Experiences in Humanities
Authors: Romo-De León Rebeca , Cham-Pérez Mei Li L. , Elizondo-Villegas Verónica Andrea , Villarreal-Villarreal Alejandro , Ortiz-Espinoza Alexandro Antonio , Vélez-Saboyá Carol Stefany , Lozoya-Santos Jorge de Jesús , Cebral-Loureda Manuel , Ramírez-Moreno Mauricio A.
Published: May 2024

The relevance of the interaction between Humanities-enhanced learning using immersive environments and simultaneous physiological signal analysis contributes to the development of Neurohumanities and advancements in applications of Digital Humanities. The present dataset consists of recordings from 24 participants divided in two groups (12 participants in each group) engaging in simulated learning scenarios, traditional learning, and partially immersive learning experiences. Data recordings from each participant contain recordings of physiological signals and psychometric data collected from applied questionnaires. Physiological signals include electroencephalography, real-time engagement and emotion recognition calculation by a Python EEG acquisition code, head acceleration, electrodermal activity, blood volume pressure, inter-beat interval, and temperature. Before the acquisition of physiological signals, participants were asked to fill out the General Health Questionnaire and Trait Meta-Mood Scale. In between recording sessions, participants were asked to fill out Likert-scale questionnaires regarding their experience and a Self-Assessment Manikin. At the end of the recording session, participants filled out the ITC Sense of Presence Inventory questionnaire for user experience. The dataset can be used to explore differences in physiological patterns observed between different learning modalities in the Humanities.
Prevalence of Peripheral Arterial Disease and Principal Associated Risk Factors in Patients with Type 2 Diabetes Mellitus: The IDON-Peripheral Arterial Disease Study
Authors: Ascencio Aliyanet Isamara Porcayo , Carmona Evangelina Morales , Farías Jesús Morán , Medina Dulce Stephanie Guzmán , Salas Rebeca Galindo , Sauque Reyna Leobardo
Published: May 2024

The principal purpose of this study is to determine the prevalence of peripheral arterial disease (PAD), as well as the principal associated risk factors, in patients registered in the IDON-PAD database. PAD is a condition characterized by the narrowing or blockage of arteries in the body’s extremities due to plaque buildup, leading to reduced blood flow and tissue ischemia. While PAD primarily affects the lower extremities, it can lead to symptoms such as intermittent claudication and, in severe cases, ulcers and amputations. Risk factors for PAD are numerous and cumulative, including smoking, age over 50, type 2 diabetes mellitus, and hypertension. The prevalence of PAD increases with age, with rates ranging from 2.5% in those over 50 to 60% in those over 85, varying by ethnicity and study population. Diabetic patients face a higher risk of PAD-related complications and have lower success rates with revascularization procedures. The diagnosis of PAD traditionally relied on physical examination and symptoms, but the Ankle–Brachial Index is now a standard diagnostic tool due to its non-invasive nature and reliability. In Mexico, the prevalence of PAD is estimated at 10%, with significant risk factors being the duration of diabetes, hypertension, hypertriglyceridemia, and smoking. Notably, 70% of PAD cases are asymptomatic, emphasizing the importance of proactive screening. This study aimed to determine the prevalence of PAD and associated risk factors in diabetic patients aged 40 and above. The prevalence was found to be 11.2%, with high-risk waist circumference, elevated triglycerides, positive Edinburgh questionnaire, and weak pulses as significant predictors. The detection and management of PAD in diabetic patients require a comprehensive approach, including lifestyle modifications and regular screenings. Prevention strategies should focus on controlling risk factors, including obesity, hypertension, and dyslipidemia. In conclusion, PAD is a prevalent yet underdiagnosed condition in diabetic patients, necessitating proactive screening and comprehensive management to mitigate associated risks and improve patient outcomes. The principal limitation of this study is that, as it uses a cross-sectional methodology and is not an experimental study, although we can establish the prevalence of PAD as well as the associated risk factors, we cannot define causality or determine the hazard ratio for each of these factors. Special thanks to Dr. Leobardo Sauque Reyna and all participants for their contribution to this research.
Deep Reinforcement Learning-Based 3D Trajectory Planning for Cellular Connected UAV
Authors: Liu Xiang , Zhong Weizhi , Wang Xin , Duan Hongtao , Fan Zhenxiong , Jin Haowen , Huang Yang , Lin Zhipeng
Published: May 2024

To address the issue of limited application scenarios associated with connectivity assurance based on two-dimensional (2D) trajectory planning, this paper proposes an improved deep reinforcement learning (DRL) -based three-dimensional (3D) trajectory planning method for cellular unmanned aerial vehicles (UAVs) communication. By considering the 3D space environment and integrating factors such as UAV mission completion time and connectivity, we develop an objective function for path optimization and utilize the advanced dueling double deep Q network (D3QN) to optimize it. Additionally, we introduce the prioritized experience replay (PER) mechanism to enhance learning efficiency and expedite convergence. In order to further aid in trajectory planning, our method incorporates a simultaneous navigation and radio mapping (SNARM) framework that generates simulated 3D radio maps and simulates flight processes by utilizing measurement signals from the UAV during flight, thereby reducing actual flight costs. The simulation results demonstrate that the proposed approach effectively enable UAVs to avoid weak coverage regions in space, thereby reducing the weighted sum of flight time and expected interruption time.
Implementation of Inhaled Methoxyflurane for the Reduction of Distal Radius Fractures in a Third-Level Hospital: A Descriptive Cohort Study
Authors: Albavera-Gutierrez Roberto R. , Paredes-Herrera Francisco J. , Gasca-López Gilberto A. , Waldo-Castañón Zuri , Espinosa-Ramos Manuel A. , Valencia-Ledezma Omar Esteban , Castro-Fuentes Carlos Alberto
Published: May 2024

Orthopedic emergencies due to fractures are one of the main reasons for surgical intervention. The distal radius is a structure prone to fractures, particularly in young people. The main objective of this study was to evaluate the success rate of methoxyflurane in the reduction in distal radius fractures that required conservative treatment. The medical records of all the patients with Frykman type I and type II distal radius fractures who underwent fracture reduction with methoxyflurane or propofol from December 2021 to January 2023 were reviewed. The success rate of distal radius fracture reduction with methoxyflurane was analyzed, and the secondary outcomes, such as the recovery time and length of stay in the orthopedic emergency department, were assessed and compared with propofol. A total of 145 patients with distal radius fractures presented to the emergency department during the study period. Seventy-five patients met our inclusion criteria. Fifty patients had a fracture reduction with methoxyflurane, while twenty patients received propofol. The median recovery time and reduction duration were 30 min and 65.5 min, respectively, resulting in a shorter period for the methoxyflurane group. Methoxyflurane was successfully used in 48 of the 82 patients undergoing Frykman type I and type II distal radius fracture reduction while improving the recovery time due to its easy self-delivery and rapid effectiveness in the patient, as well as being able to estimate the level of pain so that the amount of anesthetic needed can be estimated and adverse effects avoided. Methoxyflurane represents a great candidate for surgical interventions like the reduction in distal radius fractures.
Why Do Vocational High School Students Opt for College?
Authors: Seo Wonseok , Lee Changhoon
Published: May 2024

This study aims to elucidate how—and the underlying significance of their doing so—Korean vocational high school students decide to pursue university education rather than entering the workforce. Drawing on autoethnographic journals, the research employs a combination of Chang’s descriptive-realistic, confessional-emotive, and analytical-interpretive writing methods to convey personal experiences, including the background of students and their motivations for opting for university. As an autoethnographer, I have encountered the societal perceptions of Korean vocational high schools, issues with school curricula, and misunderstandings surrounding employment. Over time, I chronicled the documented process of choosing university education, and this has been facilitated by conversations with the homeroom teacher; I have endeavored to elucidate the sociocultural implications of each student’s experience through interpretive methods. This study’s findings are anticipated to enhance the understanding of the fundamentals of career education in the realm of secondary vocational education and to offer a valuable reference for vocational high school educators on how to guide their students effectively. Furthermore, it should provide educational perspectives and fresh insights for vocational educators and researchers worldwide, thereby facilitating enhancements in career education policies and systems for vocational students.
A Single-Buyer Model of Imbalance Cost Pass-Through Pricing Forecasting in the Malaysian Electricity Supply Industry
Authors: Khairuddin Fatin Khairunnisa , Zaini Farah Anishah , Sulaima Mohamad Fani , Shamsudin Nur Hazahsha , Abu Hanifah Mohd Shahrin
Published: May 2024

The imbalance cost pass-through (ICPT) is a flexible component of the incentive-based regulation (IBR) that empowers power producers to adjust tariffs in response to variable fuel prices, thereby enhancing the economic resilience of electricity generation. In Malaysia, the Energy Commission has conducted biannual reviews of fuel and other generation costs. Any cost savings or increases identified during these reviews will be passed on to customers in the form of rebates or surcharges. Meanwhile, if an increment in the ICPT price signal can be provided to electricity providers and consumers, early preparation for operation budgeting can be realised, and energy management program development can be properly prepared. Due to this reason, this study proposes ICPT price forecasting for the electricity market in Peninsular Malaysia that will benefit the stakeholders. The study aims to construct an ICPT-related baseline model for the peninsular generation data by employing three forecasting methods. The forecasting performance is analysed using the mean absolute percentage error (MAPE). In light of our findings, the ARIMA method is one of the most accurate forecasting methods for fuel prices compared to the moving average (MA) and LSSVM methods. The observed price differences between the ARIMA and LSSVM models for ICPT are minimal. The ICPT price for July–December 2022 and January–June 2023 is MYR 0.21/kWh for the ARIMA and MYR 0.18/kWh for LSSVM, which are close to the actual TNB’s ICPT tariff. As for forecasting, the ICPT price is expected to drop in the next announcement. The findings of this study may have a positive impact on the sustainability of the energy sector in Malaysia.
Optimized Feeding Strategies for Biosurfactant Production from Acetate by Alcanivorax borkumensis SK2
Authors: Karmainski Tobias , Lipa Marie K. , Kubicki Sonja , Bouchenafa Amina , Thies Stephan , Jaeger Karl-Erich , Blank Lars M. , Tiso Till
Published: May 2024

Biosurfactants are much-discussed alternatives to petro- and oleochemical surfactants. Alcanivorax borkumensis, a marine, Gram-negative γ-proteobacterium, produces a glycine-glucolipid biosurfactant from hydrocarbons, pyruvate, and acetate as carbon sources. Sustainable acetate production from lignocellulose or syngas adds to its relevance for the bioeconomy. This study investigated nitrogen sources and carbon-to-nitrogen ratios (C/N) to optimize fed-batch fermentation for biosurfactant production using A. borkumensis with acetate as the carbon source. Urea enabled high biosurfactant production, which was confirmed in DO-based fed-batch fermentation. Varying C/N ratios led to increased glycine-glucolipid production and decreased biomass production, with improvement plateauing at a C/N ratio of 26.7 Cmol Nmol−1. pH-stat fed-batch fermentation using glacial acetic acid as the carbon source and a pH-adjusting agent doubled the biosurfactant production. Finally, bubble-free membrane aeration was used to prevent extensive foam formation observed during conventional bubble aeration. The efficient production made it possible to investigate the bioactivity of glycine-glucolipid in combination with antibiotics against various microorganisms. Our findings allow for the leverage of glycine-glucolipid biosurfactant production using acetate as a carbon source.
Investigation of the Antiviral Mechanism of Curcumin Analog EF-24 against Siniperca cachuatsi Rhabdovirus
Authors: Ju Pei-Mo , Ma Shi-Wei , Li Yi-Yang , Zhang Shi-Fan , Li Jun , Zhou Guang-Zhou
Published: May 2024

Siniperca chuatsi rhabdovirus (SCRV) is a major strain of viral fish virus resulting in multiple transmissions and devastating damage in aquaculture. Currently, there are no available approved therapeutics. In this study, we screened and identified a novel curcumin analog (EF-24) for evaluating its in vitro anti-SCRV properties and potential molecular mechanisms. Present results demonstrated that EF-24 could strongly delay the occurrence of cytopathic effects (CPEs) in epithelioma papulosum cyprinid cells (EPCs) and inhibit SCRV replication and viral nucleoprotein expression in the early stages of infection by the time-of-addition assay. Furthermore, flow cytometry analysis after Annexin V-FITC/PI double staining and immunofluorescence microscopy observation after JC-1 incubation showed that EF-24 downregulated cell mitochondrial apoptosis induced by SCRV. The enzymatic activities of caspase-3 and caspase-9 were also reduced after EF-24 treatment, indicating that EF-24 may protect cells from SCRV infection by decreasing mitochondrial intrinsic apoptosis in infected cells. Collectively, we demonstrated for the first time that the curcumin analog EF-24 possesses antiviral ability against SCRV, suggesting its potential for effective control of fish rhabdovirus spreading.
Exponential H∞ Output Control for Switching Fuzzy Systems via Event-Triggered Mechanism and Logarithmic Quantization
Authors: Ren Jiaojiao , Zhao Can , Xiao Jianying , Luo Renfu , He Nanrong
Published: May 2024

This paper investigates the problem of exponential H∞ output control for switching fuzzy systems, considering both impulse and non-impulse scenarios. Unlike previous research, where the average dwell time (ADT: τa) and the upper bound of inter-event intervals (IEIs: T) satisfy the condition τa≥lnμ+(α+β)Tα=lnμ+βTα+T, implying that frequent switching is difficult to achieve, this paper demonstrates that by adopting the mode-dependent event-triggered mechanism (ETM) and a switching law, frequent switching is indeed achieved. Moreover, the question of deriving the normal L2 norm constraint is solved through the ADT method, although only a weighted L2 norm constraint was obtained previously. Additionally, by constructing a controller-mode-dependent Lyapunov function and adopting logarithmic quantizers, the sufficient criteria of exponential H∞ output control problem are presented. The validity of established results is demonstrated by a given numerical simulation.
Unstable Metal Hydrides for Possible On-Board Hydrogen Storage
Authors: Cao Zhijie , Habermann Franziska , Burkmann Konrad , Felderhoff Michael , Mertens Florian
Published: May 2024

Hydrogen storage in general is an indispensable prerequisite for the introduction of a hydrogen energy-based infrastructure. In this respect, high-pressure metal hydride (MH) tank systems appear to be one of the most promising hydrogen storage techniques for automotive applications using proton exchange membrane (PEM) fuel cells. These systems bear the potential of achieving a beneficial compromise concerning the comparably large volumetric storage density, wide working temperature range, comparably low liberation of heat, and increased safety. The debatable term “unstable metal hydride” is used in the literature in reference to metal hydrides with high dissociation pressure at a comparably low temperature. Such compounds may help to improve the merits of high-pressure MH tank systems. Consequently, in the last few years, some materials for possible on-board applications in such tank systems have been developed. This review summarizes the state-of-the-art developments of these metal hydrides, mainly including intermetallic compounds and complex hydrides, and offers some guidelines for future developments. Since typical laboratory hydrogen uptake measurements are limited to 200 bar, a possible threshold for defining unstable hydrides could be a value of their equilibrium pressure of peq > 200 bar for T < 100 °C. However, these values would mark a technological future target and most current materials, and those reported in this review, do not fulfill these requirements and need to be seen as current stages of development toward the intended target. For each of the aforementioned categories in this review, special care is taken to not only cover the pioneering and classic research but also to portray the current status and latest advances. For intermetallic compounds, key aspects focus on the influence of partial substitution on the absorption/desorption plateau pressure, hydrogen storage capacity and hysteresis properties. For complex hydrides, the preparation procedures, thermodynamics and theoretical calculation are presented. In addition, challenges, perspectives, and development tendencies in this field are also discussed.
Air Quality Monitoring Using Low-Cost Sensors in Urban Areas of Jodhpur, Rajasthan
Authors: Huda Ramesh Kumar , Kumar Pankaj , Gupta Rajnish , Sharma Arun Kumar , Toteja G. S. , Babu Bontha V.
Published: May 2024

Air pollution poses a significant health hazard in urban areas across the globe, with India being one of the most affected countries. This paper presents environmental monitoring study conducted in Jodhpur, Rajasthan, India, to assess air quality in diverse urban environments. The study involved continuous indoor and outdoor air quality monitoring, focusing on particulate matter (PM2.5) levels, bioaerosols, and associated meteorological parameters. Laser sensor-based low-cost air quality monitors were utilized to monitor air quality and Anderson 6-stage Cascade Impactor & Petri Dish methods for bioaerosol monitoring. The study revealed that PM2.5 levels were consistently high throughout the year, highlighting the severity of air pollution in the region. Notably, indoor PM2.5 levels were often higher than outdoor levels, challenging the common notion of staying indoors during peak pollution. The study explored the spatial and temporal diversity of air pollution across various land-use patterns within the city, emphasizing the need for tailored interventions in different urban areas. Additionally, bioaerosol assessments unveiled the presence of pathogenic organisms in indoor and outdoor environments, posing health risks to residents. These findings underscore the importance of addressing particulate matter and bioaerosols in air quality management strategies. Despite the study’s valuable insights, limitations, such as using low-cost air quality sensors and the need for long-term data collection, are acknowledged. Nevertheless, this research contributes to a better understanding of urban air quality dynamics and the importance of public awareness in mitigating the adverse effects of air pollution. In conclusion, this study underscores the urgent need for effective air quality management strategies in urban areas. The findings provide valuable insights for policymakers and researchers striving to address air pollution in rapidly urbanizing regions.
Radiogenomics Map-Based Molecular and Imaging Phenotypical Characterization in Localised Prostate Cancer Using Pre-Biopsy Biparametric MR Imaging
Authors: Ogbonnaya Chidozie N. , Alsaedi Basim S. O. , Alhussaini Abeer J. , Hislop Robert , Pratt Norman , Steele J. Douglas , Kernohan Neil , Nabi Ghulam
Published: May 2024

To create a radiogenomics map and evaluate the correlation between molecular and imaging phenotypes in localized prostate cancer (PCa), using radical prostatectomy histopathology as a reference standard. Radiomic features were extracted from T2-weighted (T2WI) and Apparent Diffusion Coefficient (ADC) images of clinically localized PCa patients (n = 15) across different Gleason score-based risk categories. DNA extraction was performed on formalin-fixed, paraffin-embedded (FFPE) samples. Gene expression analysis of androgen receptor expression, apoptosis, and hypoxia was conducted using the Chromosome Analysis Suite (ChAS) application and OSCHIP files. The relationship between gene expression alterations and textural features was assessed using Pearson’s correlation analysis. Receiver operating characteristic (ROC) analysis was utilized to evaluate the predictive accuracy of the model. A significant correlation was observed between radiomic texture features and copy number variation (CNV) of genes associated with apoptosis, hypoxia, and androgen receptor (p-value ≤ 0.05). The identified radiomic features, including Sum Entropy ADC, Inverse Difference ADC, Sum Variance T2WI, Entropy T2WI, Difference Variance T2WI, and Angular Secondary Moment T2WI, exhibited potential for predicting cancer grade and biological processes such as apoptosis and hypoxia. Incorporating radiomics and genomics into a prediction model significantly improved the prediction of prostate cancer grade (clinically significant prostate cancer), yielding an AUC of 0.95. Radiomic texture features significantly correlate with genotypes for apoptosis, hypoxia, and androgen receptor expression in localised prostate cancer. Integration of these into the prediction model improved prediction accuracy of clinically significant prostate cancer.
Anticancer Effects of the Novel Pyrazolyl-Urea GeGe-3
Authors: Williams Ashleigh , Cooper Emma , Clark Bethany , Perry Laura , Ponassi Marco , Iervasi Erika , Brullo Chiara , Greenhough Alexander , Ladomery Michael
Published: May 2024

In a screen of over 200 novel pyrazole compounds, ethyl 1-(2-hydroxypentyl)-5-(3-(3-(trifluoromethyl) phenyl)ureido)-1H-pyrazole-4-carboxylate (named GeGe-3) has emerged as a potential anticancer compound. GeGe-3 displays potent anti-angiogenic properties through the presumptive targeting of the protein kinase DMPK1 and the Ca2+-binding protein calreticulin. We further explored the anticancer potential of GeGe-3 on a range of established cancer cell lines, including PC3 (prostate adenocarcinoma), SKMEL-28 (cutaneous melanoma), SKOV-3 (ovarian adenocarcinoma), Hep-G2 (hepatocellular carcinoma), MDA-MB231, SKBR3, MCF7 (breast adenocarcinoma), A549 (lung carcinoma), and HeLa (cervix epithelioid carcinoma). At concentrations in the range of 10 μM, GeGe-3 significantly restricted cell proliferation and metabolism. GeGe-3 also reduced PC3 cell migration in a standard wound closure and trans-well assay. Together, these results confirm the anticancer potential of GeGe-3 and underline the need for more detailed pre-clinical investigations into its molecular targets and mechanisms of action.
Recent Approaches to Wound Treatment—Second Edition
Authors: Pagano Cinzia , Iborra César Antonio Viseras , Perioli Luana
Published: May 2024

Wound healing is a complex process involving a number of mechanisms [...]
Special Issue: Molecular Ecology, Physiology and Biochemistry of Insects 3.0
Authors: Hoffmann Klaus H.
Published: May 2024

With the outstanding work of Sir Vincent B [...]