bluuebunny/crossref_metadata_2025 huggingface dataset:
Electrical Characteristics of Solution-Based Thin-Film Transistors with a Zinc-Tin Oxide/Carbon Nanotube Stacked Nanocomposite Active Layer
Authors: Kim Yong-Jae , Choi Woon-Seop
Published: December 2024

A stacked nanocomposite zinc-tin oxide/single-walled carbon nanotubes (ZTO/SWNTs) active layer was fabricated for thin-film transistors (TFTs) as an alternative to the conventional single-layer structure of mixed ZTO and SWNTs. The stacked nanocomposite of the solution-processed TFTs was prepared using UV/O3 treatment and multiple annealing steps for each layer. The electrical properties of the stacked device were superior to those of the single-layer TFT. The ZTO/SWNT TFT, fabricated using a stacked structure with ZTO on the top and SWNT at the bottom layer, showed a significant improvement in the field-effect mobility of 15.37 cm2/V·s (factor of three increase) and an Ion/Ioff current ratio of 8.83 × 108 with improved hysteresis. This outcome was attributed to the surface treatment and multiple annealing of the selected active layer, resulting in improved contact and a dense structure. This was also attributed to the controlled dispersion of SWNT, as electron migration paths without dispersants. This study suggests the potential expansion of applications, such as flexible electronics and low-cost fabrication of TFTs.
Biochar in the Remediation of Organic Pollutants in Water: A Review of Polycyclic Aromatic Hydrocarbon and Pesticide Removal
Authors: Beljin Jelena , Đukanović Nina , Anojčić Jasmina , Simetić Tajana , Apostolović Tamara , Mutić Sanja , Maletić Snežana
Published: December 2024

This review explores biochar’s potential as a sustainable and cost-effective solution for remediating organic pollutants, particularly polycyclic aromatic hydrocarbons (PAHs) and pesticides, in water. Biochar, a carbon-rich material produced from biomass pyrolysis, has demonstrated adsorption efficiencies exceeding 90% under optimal conditions, depending on the feedstock type, pyrolysis temperature, and functionalization. High surface area (up to 1500 m2/g), porosity, and modifiable surface functional groups make biochar effective in adsorbing a wide range of contaminants, including toxic metals, organic pollutants, and nutrients. Recent advancements in biochar production, such as chemical activation and post-treatment modifications, have enhanced adsorption capacities, with engineered biochar achieving superior performance in treating industrial, municipal, and agricultural effluents. However, scaling up biochar applications from laboratory research to field-scale wastewater treatment poses significant challenges. These include inconsistencies in adsorption performance under variable environmental conditions, the high cost of large-scale biochar production, logistical challenges in handling and deploying biochar at scale, and the need for integration with existing treatment systems. Such challenges impact the practical implementation of biochar-based remediation technologies, requiring further investigation into cost-effective production methods, long-term performance assessments, and field-level optimization strategies. This review underscores the importance of addressing these barriers and highlights biochar’s potential to offer a sustainable, environmentally friendly, and economically viable solution for large-scale wastewater treatment.
Effects of Long-Term Fasting on Gut Microbiota, Serum Metabolome, and Their Association in Male Adults
Authors: Wu Feng , Guo Yaxiu , Wang Yihua , Sui Xiukun , Wang Hailong , Zhang Hongyu , Xin Bingmu , Yang Chao , Zhang Cheng , Jiang Siyu , Qu Lina , Feng Qiang , Dai Zhongquan , Shi Chunmeng , Li Yinghui
Published: December 2024

Background: Long-term fasting demonstrates greater therapeutic potential and broader application prospects in extreme environments than intermittent fasting. Method: This pilot study of 10-day complete fasting (CF), with a small sample size of 13 volunteers, aimed to investigate the time-series impacts on gut microbiome, serum metabolome, and their interrelationships with biochemical indices. Results: The results show CF significantly affected gut microbiota diversity, composition, and interspecies interactions, characterized by an expansion of the Proteobacteria phylum (about six-fold) and a decrease in Bacteroidetes (about 50%) and Firmicutes (about 34%) populations. Notably, certain bacteria taxa exhibited complex interactions and strong correlations with serum metabolites implicated in energy and amino acid metabolism, with a particular focus on fatty acylcarnitines and tryptophan derivatives. A key focus of our study was the effect of Ruthenibacterium lactatiformans, which was highly increased during CF and exhibited a strong correlation with fat metabolic indicators. This bacterium was found to mitigate high-fat diet-induced obesity, glucose intolerance, dyslipidemia, and intestinal barrier dysfunction in animal experiments. These effects suggest its potential as a probiotic candidate for the amelioration of dyslipidemia and for mediating the benefits of fasting on fat metabolism. Conclusions: Our pilot study suggests that alterations in gut microbiota during CF contribute to the shift of energy metabolic substrate and the establishment of a novel homeostatic state during prolonged fasting.
Beyond Borders: Investigating the Impact of COVID-19 Anxiety and Eating Attitudes on Psychological Well-Being and Physical Activity Objectives in Poland and China
Authors: Li Jianye , Wilczyńska Dominika Maria , Lipowska Małgorzata , Łada-Maśko Ariadna Beata , Radtke Bartosz M. , Sajewicz-Radtke Urszula , Izydorczyk Bernadetta , Liu Taofeng , Wang Zitong , Lu Junyu , Lipowski Mariusz
Published: December 2024

Background/Objectives: The mechanisms linking eating attitudes to well-being and physical activity objectives have increasingly attracted the attention of researchers in recent years. This research is particularly significant in the context of the COVID-19 pandemic, which has profoundly disrupted eating habits, exercise routines, and psychosocial well-being across the globe. Additionally, these variables are influenced by cultural dimensions, such as individualism in Poland and collectivism in China. These two countries represent distinct approaches to social health and well-being during the pandemic, offering valuable comparative insights into how cultural contexts shape mental and physical health behaviors; Methods: The study included 644 Polish and 690 Chinese participants. It utilized the Psychological Well-Being Scale (PWBS), the Coronavirus Anxiety Scale (CAS), the Eating Attitudes Test (EAT-26), and the Inventory of Physical Activity Objectives (IPAO); Results: The results indicate that both COVID-19 anxiety and eating attitudes fully mediate the relationship between well-being and physical activity objectives. Full mediation implies that the observed relationship between well-being and physical activity objectives operates entirely through the mediators. Notably, the mediating effect of COVID-19 anxiety was observed only in the Chinese sample, highlighting cultural differences in coping mechanisms and societal responses to anxiety. Cultural differences significantly influenced well-being and physical activity objectives, while eating disorders remained unaffected by cultural and social differences. Additionally, a significant positive correlation was found between COVID-19 anxiety, eating attitudes, and health-related physical activity objectives, underscoring the interplay between mental health and physical activity; Conclusions: These findings underscore the importance of addressing anxiety and eating attitudes to enhance well-being and physical activity behaviors. The study provides a strong theoretical basis for targeted interventions tailored to cultural contexts. Potential limitations include the reliance on self-reported data and differences in demographic characteristics between the Polish and Chinese samples, which may affect generalizability.
The Effectiveness of an Online Nutrition Education Program on Varsity Athletes’ Nutritional & Dietary Supplement Knowledge
Authors: Daher Jana , Mountjoy Margo , El Khoury Dalia
Published: December 2024

Background/Objectives: Research has shown that athletes often have poor nutritional knowledge, particularly regarding dietary supplements. The purpose of this study was to investigate the effectiveness of an online nutrition education program in improving nutritional and dietary supplement knowledge among varsity athletes at the University of Guelph in Ontario, Canada. Methods: A total of 30 varsity athletes at the University of Guelph were randomized into experimental [n = 18] and control [n = 12] groups. A randomized wait-list controlled intervention was used, where participants in the experimental group were granted access to an online, 4-week nutrition education program, while no program was provided to the control group within the duration of the study. The program covered nutrition topics and focused on sports nutrition and dietary supplementation. Both groups were administered a validated online nutrition and dietary supplement questionnaire at baseline and post-intervention. Results: Mean baseline knowledge scores for the experimental and control groups were 13.78 ± 2.76 and 13.92 ± 2.39, respectively, and were not significantly different [p = 0.888]. Post-intervention scores were 16.28 ± 1.49 and 14.5 ± 1.88 for the experimental and control groups, respectively, [p < 0.05]. There was a significant interaction between the intervention and time on knowledge. Conclusions: These results indicate that this nutrition education program was successful in significantly improving nutritional and dietary supplement knowledge in varsity athletes.
Macronutrients and Micronutrients in Parenteral Nutrition
Authors: Verlato Giovanna , Meneghelli Marta , Cavicchiolo Maria Elena
Published: December 2024

Appropriate nutrition is of paramount importance during infancy and childhood, and Parenteral Nutrition (PN), which is the intravenous infusion of nutrients in the elementary form, may be necessary as a supplement or a full replacement for enteral nutrition [...]
Independent and Combined Associations of Physical Activity in Different Domains and Inflammatory Diet with Type 2 Diabetes: A Population-Based Cohort Study
Authors: Zhou Jianfan , Yin Shuting , Du Litao , Xue Xiangli , He Qiang , Zhao Na , Chen Si , Zhang Xianliang
Published: December 2024

Objective: This study aims to explore the independent and combined associations of physical activity (PA) in different domains and inflammatory diet with type 2 diabetes mellitus (T2DM). Methods: Data from 8736 American adults from the NHANES 2007–2016 were used. PA in different domains was assessed using the self-reported Global Physical Activity Questionnaire, and dietary inflammatory index was estimated based on 24 h dietary recalls. T2DM diagnosis was determined by a combination of self-report and laboratory data. A multivariate modified Poisson regression model was used to explore the independent and combined associations of moderate-vigorous intensity physical activity (MVPA) and inflammatory diet with T2DM. Results: PA in the Work MVPA, Recreational MVPA and Total MVPA domains was independently associated with reduced risk of T2DM, and an inflammatory diet was independently associated with elevated risk of T2DM. In the combined analysis, the combination of active and anti-inflammatory within the Work MVPA, Recreational MVPA and Total MVPA fields was associated with the greatest reduced risk of T2DM, and always associated with decreased risk of T2DM in the active group. Conclusions: Our study emphasizes that the combination of active PA and anti-inflammatory diet is closely associated with the reduced risk of T2DM, and suggests the combination of both for the prevention and treatment of T2DM.
Dietary Bioactive Compounds and Human Health: The Role of Bioavailability
Authors: Vieira Italo Rennan Sousa , Conte-Junior Carlos Adam
Published: December 2024

The relationship between dietary bioactive compounds and human health has generated significant interest among members of the scientific community and the general public [...]
The Impact of Hypomagnesemia on the Long-Term Evolution After Kidney Transplantation
Authors: Ratiu Ioana Adela , Moisa Corina , Marc Luciana , Olariu Nicu , Ratiu Cristian Adrian , Bako Gabriel Cristian , Ratiu Anamaria , Fratila Simona , Teusdea Alin Cristian , Ganea Mariana , Indries Mirela , Filip Lorena
Published: December 2024

Background/Objectives: Magnesium plays a crucial role in immune function, influencing immunoglobulin synthesis, antibody-dependent cytolysis, and other immune processes. In renal transplant patients, magnesium deficiency is primarily induced by calcineurin inhibitor treatment, through the reduction of magnesium transporter proteins in the renal tubules, leading to magnesium loss. Methods: To assess the correlation between serum magnesium levels and the long-term outcomes of renal graft and transplant recipients, we conducted a retrospective study on 87 patients who have had a transplant for more than 5 years, a period considered immunologically stable. We evaluated laboratory parameters such as glycemia, creatinine, total protein, and C-reactive protein (CRP), as well as demographic data, primary kidney disease, donor type, comorbidities, and infection incidence. Results: This study revealed clinical stability at over 5 years post-transplantation, with no significant differences between the 5–15 and over-15-years groups with regard to major comorbidities, except for HCV infection (p = 0.018). Reduced magnesium levels were associated with impaired renal function (p = 0.017) and inflammatory syndrome (p = 0.012). Viral infections were correlated with living donor grafts (p = 0.05), hypoproteinemia, and decreased eGFR (estimated glomerular filtration rate), while bacterial infections, namely urinary tract infections (UTIs), were linked to reduced eGFR (p = 0.05, p = 0.046). Female patients with hypomagnesemia had a higher incidence of recurrent UTIs (p = 0.03). Conclusions: Hypomagnesemia correlates with increased infection risk in patients who received a renal transplant more than 5 years ago but does not significantly impact glycemic control or cardiovascular health.
Probiotics: A Potential Strategy for Preventing and Managing Cardiovascular Disease
Authors: López-Yerena Anallely , de Santisteban Villaplana Victoria , Badimon Lina , Vilahur Gemma , Padro Teresa
Published: December 2024

Probiotics are gaining recognition as a viable strategy for mitigating cardiovascular risk factors. Specifically, recent studies highlight their potential benefits in managing cholesterol levels, blood pressure, and inflammation, which are critical components in the prevention of cardiovascular diseases (CVD). This comprehensive review aims to elucidate the impact of probiotic consumption on major cardiovascular risk factors, including individuals with hypertension, type II diabetes mellitus, metabolic syndrome, hypercholesterolemia, and in secondary prevention in coronary artery disease. Scientific evidence based on human studies suggests that probiotic consumption is associated with positive effects on anthropometric measures, inflammation markers, blood pressure, glucose metabolism markers, lipid profiles, and endothelial function. However, these findings should be interpreted pragmatically and acknowledge the significant variability in results. This variability may be attributed to factors such as probiotic composition (single strain or multiple strains), the characteristics of the delivery matrix (food, capsules, and sachets), the duration of the intervention, the dosage regimen, and baseline health profiles of the participants. Incorporating probiotics as part of a comprehensive and healthy lifestyle approach can be considered a feasible strategy for both the prevention and management of CVD. However, further research is needed on factors influencing the effect of probiotics, such as: (i) optimal probiotic strain(s), (ii) appropriate dosage, (iii) duration of treatment, (iv) optimal delivery vehicle, and (v) sex-specific differences.
Satisfaction Levels of Ambulatory Patients with the Quality of Nursing Care: Validation and Application of the Patient Satisfaction with Nursing Care Quality Questionnaire in Albania
Authors: Qirko Sonila , Prifti Vasilika , Kicaj Emirjona , Çerçizaj Rudina , Rogozea Liliana Marcela
Published: December 2024

Background: In the last decades, there has been a growing demand for outpatient services; understanding the factors influencing patient satisfaction is critical for improving healthcare quality. Objectives: This study evaluates patient satisfaction with nursing care and examines how satisfaction varies based on socio-demographic factors in ambulatory settings across five healthcare centers in the municipality of Vlora, Albania. Methods: In this cross-sectional study, a total of 246 patients were surveyed using the Patient Satisfaction with Nursing Care Quality Questionnaire (PSNCQQ), adapted for outpatient contexts, after assessing its validity and reliability. The mean age of the sample was 63.9 ± 13.1 years old with a range of 21 to 94 years, and 47.2% were aged between 50 and 69 years. Results: The results indicate that the overall satisfaction level was fair, with a mean PSNCQQ score of 2.55 on a five-point scale. Socio-demographic factors, such as age, gender, education, and socio-economic status, significantly impacted patient satisfaction. Younger patients (aged 20–49), females, and those with a higher education and socio-economic status reported higher satisfaction. Medical history also played a role, with patients attending general check-ups showing greater satisfaction compared to those with chronic conditions. Older patients tend to report a lower level of satisfaction with the care provided compared to younger patients. Conclusions: Communication and nurse–patient interactions emerged as key areas for improvement, particularly in outpatient settings where care is episodic. These findings suggest that personalized care, improved communication, and greater attention to socio-demographic and medical factors can enhance patient satisfaction in ambulatory care settings.
(-)-Fenchone Ameliorates TNBS-Induced Colitis in Rats via Antioxidant, Immunomodulatory, and Cytoprotective Mechanisms
Authors: Araruna Maria Elaine Cristina , Alves Júnior Edvaldo Balbino , de Lima Serafim Catarina Alves , Pessoa Matheus Marley Bezerra , de Souza Pessôa Michelle Liz , Alves Vitória Pereira , Sobral Marianna Vieira , da Silva Marcelo Sobral , Alves Adriano Francisco , de Paiva Sousa Maria Carolina , Araújo Aurigena Antunes , Batista Leônia Maria
Published: December 2024

Background: (-)-Fenchone is a bicyclic monoterpene present in the plant species Foeniculum vulgare Mill, Thuja occidentalis L. (tuja), and Lavandula stoechas (lavender). These plants have therapeutic value in the treatment of intestinal disorders. Aim: To evaluate intestinal anti-inflammatory activity in an acute and chronic trinitrobenzene sulphonic acid (TNBS)-induced colitis model in rats. Methods: Intestinal anti-inflammatory effects were assessed using the acute and chronic TNBS-induced colitis model in rats. The mechanisms were evaluated from colonic tissue fragments of the acute and chronic models. Results: Oral administration of the (-)-fenchone (37.5–300 mg/kg) acute phase or (150 mg/kg) (p < 0.001) chronic phase reduced the macroscopic lesion score, ulcerative area, intestinal weight/length ratio, and diarrheal index in TNBS-treated animals. At a dose of 150 mg/kg, the acute and chronic phase decreased malondialdehyde (MDA) and myeloperoxidase (MPO) (p < 0.001), restored glutathione (GSH) levels and superoxide dismutase (SOD) (p < 0.001), decreased immunomarking for factor nuclear kappa B (NF-κB) and levels of interleukin (IL)-1 and tumor necrosis factor α (TNF-α), and maintained IL-10 and TGF-β basal levels. Furthermore, increased immunostaining for zonula occludens 1 (ZO-1) was observed. Conclusions: (-)-fenchone has intestinal anti-inflammatory activity related to cytoprotection of the intestinal barrier, as well as antioxidant and immunomodulatory effects.
Repurposing the Antidiabetic Drugs Glyburide, Gliquidone, and Glipizide in Combination with Benznidazole for Trypanosoma cruzi Infection
Authors: Vázquez Citlali , Encalada Rusely , Jiménez-Galicia Isabel , Gómez-Escobedo Rogelio , Rivera Gildardo , Nogueda-Torres Benjamín , Saavedra Emma
Published: December 2024

Infection with the protozoan parasite Trypanosoma cruzi causes human Chagas disease. Benznidazole (BNZ) and nifurtimox are the current drugs for the treatment; however, they induce severe adverse side effects in patients; therefore, there is a need to improve the treatment effectiveness and efficiency of these drugs for its safer use. Background/Objective: Glyburide, glipizide, and gliquidone, hypoglycemic drugs for diabetes treatment, were previously predicted to bind to dihydrofolate reductase-thymidylate synthase from T. cruzi by in silico docking analysis; they also showed antiproliferative effects against T. cruzi epimastigotes, the stage of the insect vector. In the present study, the potential parasiticidal effect of these antidiabetic drugs was tested in monotherapy and bi-therapy with BNZ in human cells in vitro and in animals. Methods: Evaluation was performed in (a) a model of in vitro infection of T. cruzi trypomastigotes using human fibroblasts as host cells and (b) in mice infected with T. cruzi. Results: The antidiabetic drugs in monotherapy showed antiparasitic effects in preventing infection progression (trypomastigotes release), with an IC50 of 8.4–14.3 µM in comparison to that of BNZ (0.26 µM) in vitro. However, in bi-therapy, the presence of just 0.5 or 1 µM of the antidiabetics decreased the BNZ IC50 by 5–10 times to 0.03–0.05 µM. Remarkably, the antidiabetic drugs in monotherapy decreased the infection in mice by 40–60% in a similar extent to BNZ (80%). In addition, the combination of BNZ plus antidiabetics perturbed the antioxidant metabolites in epimastigotes. Conclusions: These results identified antidiabetics as potential drugs in combination therapy with BNZ to treat T. cruzi infection.
Correction: Aslam et al. pH Sensitive Pluronic Acid/Agarose-Hydrogels as Controlled Drug Delivery Carriers: Design, Characterization and Toxicity Evaluation. Pharmaceutics 2022, 14, 1218
Authors: Aslam Mariam , Barkat Kashif , Malik Nadia Shamshad , Alqahtani Mohammed S. , Anjum Irfan , Khalid Ikrima , Tulain Ume Ruqia , Gohar Nitasha , Zafar Hajra , Paiva-Santos Ana Cláudia , Raza Faisal
Published: December 2024

Error in Figure [...]
Partial AUCs in Long-Acting Injectables: Rationale, Challenges, Variability, Usefulness, and Clinical Relevance
Authors: Tsakiridou Georgia , Angelerou Maria-Faidra-Galini , Efentakis Panagiotis , Margaritis Antonios , Papanastasiou Antigoni-Maria , Kalantzi Lida
Published: December 2024

Regulatory authorities typically require bioequivalence to be demonstrated by comparing pharmacokinetic parameters like area under the plasma concentration-time curve (AUC) and maximum plasma concentration (Cmax). Because in certain cases, AUC and Cmax alone may not be adequate to identify formulation differences in early and/or late segments of the dosing interval, partial AUCs (pAUCs) have been proposed as additional metrics to evaluate bioequivalence. Even though cut-off points for pAUCs are usually decided based on clinical relevance, the identification of the correct cut-off range remains elusive in many other cases and tends to contribute to increased pAUC estimate variabilities. The choice of meaningful cut-off points in pAUC estimates can be especially difficult in the case of long-acting injectable (LAI) products, where long dosing intervals and complex pharmacokinetic (PK) and pharmacodynamic (PD) profiles apply, but most importantly, because there is not always a clear PK/PD relationship established. In this communication, authors discuss the usefulness and challenges associated with the estimation of pAUCs in the development of generic LAI products through the review of six case studies under the lens of regulatory requirements from the two major authorities, namely the FDA and EMA.
Effect of Bacterial Nanocellulose with Chemisorbed Antiseptics on Alveolar Bone Repair in Rats Undergoing Bisphosphonate Therapy
Authors: Rocha Marcelo Matos , Koth Valesca Sander , Jeffman Marcela Wiltgen , Salum Fernanda Gonçalves , de Almeida Josiane , Cesca Karina , Cherubini Karen
Published: December 2024

Objectives: This work investigated the effect of bacterial nanocellulose (BNC) alone or with chemisorbed chlorhexidine or povidone-iodine on post-tooth extraction repair in rats undergoing bisphosphonate therapy. Methods: Forty Wistar rats were treated with zoledronic acid, subjected to tooth extractions and allocated into groups according to the material inserted in the post-extraction socket: (1) BNC (n = 10); (2) BNC/Iodine (n = 10); (3) BNC/Chlorhex (n = 10); (4) Control (n = 10). Maxillae were dissected and macro- and microscopically analyzed. Results: Oral lesion frequency on macroscopic examination did not differ between the groups, whereas it was larger in the BNC/Iodine group compared to the BNC/Chlorhex and Control. BNC/Chlorhex had significantly more connective tissue than did BNC but did not differ from the BNC/Iodine and Control. Epithelium, vital bone, non-vital bone, tooth fragment and inflammatory infiltrate did not significantly differ between the groups. BNC/Iodine showed greater CD31 immunostaining compared to BNC and the Control. Myeloperoxidase staining did not differ between the groups, and scanning electron microscopy analysis showed similar characteristics in all groups. Conclusions: BNC with chemisorbed povidone-iodine is associated with increased vascularization in post-extraction wounds of rats undergoing bisphosphonate therapy, whereas BNC with chemisorbed chlorhexidine improves connective tissue formation. BNC works as an effective carrier for the antiseptics tested.
T14diLys/DOPE Liposomes: An Innovative Option for siRNA-Based Gene Knockdown?
Authors: Meinhard Sophie , Erdmann Frank , Lucas Henrike , Krabbes Maria , Krüger Stephanie , Wölk Christian , Mäder Karsten
Published: December 2024

Background/Objectives: Bringing small interfering RNA (siRNA) into the cell cytosol to achieve specific gene silencing is an attractive but also very challenging option for improved therapies. The first step for successful siRNA delivery is the complexation with a permanent cationic or ionizable compound. This protects the negatively charged siRNA and enables transfection through the cell membrane. The current study explores the performance of the innovative, ionizable lipid 2-Tetradecylhexadecanoic acid-(2-bis{[2-(2,6-diamino-1-oxohexyl)amino]ethyl}aminoethyl)-amide (T14diLys), in combination with 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE), for siRNA delivery and the impact of the production method (sonication vs. extrusion) on the particle properties. Methods: Liposomes were produced either with sonication or extrusion and characterized. The extruded liposomes were combined with siRNA at different N/P ratios and investigated in terms of size zeta potential, encapsulation efficiency, lipoplex stability against RNase A, and knockdown efficiency using enhanced green fluorescent protein (eGFP)-marked colon adenocarcinoma cells. Results: The liposomes prepared by extrusion were smaller and had a narrower size distribution than the sonicated ones. The combination of siRNA and liposomes at a nitrogen-to-phosphate (N/P) ratio of 5 had optimal particle properties, high encapsulation efficiency, and lipoplex stability. Gene knockdown tests confirmed this assumption. Conclusions: Liposomes produced with extrusion were more reproducible and provided enhanced particle properties. The physicochemical characterization and in vitro experiments showed that an N/P ratio of 5 was the most promising ratio for siRNA delivery.
L-Threonine-Derived Biodegradable Polyurethane Nanoparticles for Sustained Carboplatin Release
Authors: Oh Seoeun , Park Soo-Yong , Seo Hyung Il , Chung Ildoo
Published: December 2024

Background and objectives: The use of polymeric nanoparticles (NPs) in drug delivery systems offers the advantages of enhancing drug efficacy and minimizing side effects; Methods: In this study, L-threonine polyurethane (LTPU) NPs have been fabricated by water-in-oil-in-water emulsion and solvent evaporation using biodegradable and biocompatible LTPU. This polymer was pre-synthesized through the use of an amino acid-based chain extender, desaminotyrosyl L-threonine hexyl ester (DLTHE), where urethane bonds are formed by poly(lactic acid)-poly(ethylene glycol)-poly(lactic acid) (PLA-PEG-PLA) triblock copolymer and 1,6-hexamethylene diisocyanate (HDI). LTPU is designed to be degraded by hydrolysis and enzymatic activity due to the presence of ester bonds and peptide bonds within the polymer backbone. LTPU NPs were fabricated by water-in-oil-in-water double emulsion solvent evaporation methods; Results: The polymerization of LTPU was confirmed by 1H-NMR, 13C-NMR, and FT-IR spectroscopies. The molecular weights and polydispersity, determined with GPC, were 28,800 g/mol and 1.46, respectively. The morphology and size of NPs, characterized by DLS, FE-SEM, TEM, and confocal microscopy, showed smooth and spherical particles with diameters less than 200 nm; Conclusions: In addition, the drug loading, encapsulation efficiency, and drug release profiles, using UV-Vis spectroscopy, showed the highest encapsulation efficiency with 2.5% carboplatin and sustained release profile.
Minimum-Run Resolution IV Design for Optimized Bio Removal of Fe2+ Using Enteromorpha intestinalis Aqueous Extract and Its Extract-Coated Silver Nanoparticles
Authors: El-Hosari Doaa G. , Mokhtar Fatma A. , Khalaf Hussein A. , Ibrahim Ahmed R. N. , Mohamed Rehab M. , Makhlof Mofida E. M.
Published: December 2024

Biosorbents have demonstrated considerable potential for the remediation of metals in aqueous environments. An aqueous extract of Enteromorpha intestinalis L. (EiE) and its extract-coated silver nanoparticles have been prepared and employed for the removal of iron. Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), UV-visible spectroscopy, transmission electron microscopy (TEM), gas chromatography-mass spectroscopy (GC-MS), and zeta potential were employed to characterize the prepared biosorbents. The adsorption properties of the biosorbents were investigated in batch experiments, with a range of factors taken into account, including pH, contact time, initial ion concentrations, biosorbent dosage, and temperature. A minimum-run resolution IV design (MRR-IV) was developed with the objective of optimizing the removal efficiency. The mechanisms of adsorption were investigated using both the Langmuir and Freundlich isotherms. Kinetic studies were conducted using the pseudo-first-order and pseudo-second-order models. A variety of active constituents, including organic acids, lipids, alcohols, and terpenes, were identified through the use of GC-MS, with the findings supported by FTIR spectra. Transmission electron microscopy (TEM) revealed that the nanoparticle size ranged from 5 to 44 nm, while X-ray diffraction (XRD) demonstrated a high degree of crystallinity. A screening study employing the MRR-IV methodology, facilitated by the Design-Experiment, Ver 13., indicates that three factors exert a considerable influence on the biosorption process. The study demonstrated that the biosorption mechanism is pH-dependent, with an optimal pH of 5. The adsorption performance was found to follow Freundlich isothermal models and pseudo-first-order kinetics.
Phenolic Profile and Antioxidant Capacity of Invasive Solidago canadensis L.: Potential Applications in Phytopharmacy
Authors: Uzelac Božac Mirela , Poljuha Danijela , Dudaš Slavica , Bilić Josipa , Šola Ivana , Mikulič-Petkovšek Maja , Sladonja Barbara
Published: December 2024

Canadian goldenrod (Solidago canadensis L.), an invasive plant in Europe, is known for its allelopathic activity and is rich in bioactive compounds like flavonoids and phenolic acids, with significant pharmacological potential. This study presents the LC-MS phenolic profiles of leaf and flower extracts from S. canadensis, an invasive alien plant in the Istria region (Croatia). Total phenolics (TP) (45.78–110.68 mg GAE/g DW) and non-flavonoids (TNF) (28.38–72.20 mg GAE/g DW) were found to be more abundant in ethanolic than in methanolic extracts. The antioxidant capacity (AC), as measured by ABTS, DPPH, and FRAP assays, was higher in flower extracts compared to leaf extracts. A non-targeted metabolomics approach was used, and 41 phenolic compounds in leaves and 36 in flowers were identified, with hydroxycinnamic acids and flavonols being the most abundant. 5-caffeoylquinic acid was quantitatively predominant in the leaf extracts, while quercetin-3-rutinoside dominated the flower extracts. Five leaf-specific compounds were identified (dicaffeoylquinic acid 2, 4-p-coumaroylquinic acid 1, p-hydroxybenzoic acid, quercetin-3-rhamnoside, and quercetin acetylhexoside 1), suggesting targeted extraction for different pharmacological applications. This study highlights the therapeutic potential of S. canadensis and underscores the need for further research on the bioavailability, efficacy, and safety of its compounds, potentially transforming this ecological threat into a valuable resource for drug development.
Maize Herbivore-Induced Volatiles Enhance Xenobiotic Detoxification in Larvae of Spodoptera frugiperda and S. litura
Authors: Wang Peng , Zeng Qiyue , Zhao Yi , Sun Xiaomin , Han Yongqiang , Zeng Rensen , Song Yuanyuan , Chen Dongmei , Lin Yibin
Published: December 2024

The release of herbivore-induced plant volatiles (HIPVs) has been recognized to be an important strategy for plant adaptation to herbivore attack. However, whether these induced volatiles are beneficial to insect herbivores, particularly insect larvae, is largely unknown. We used the two important highly polyphagous lepidopteran pests Spodoptera frugiperda and S. litura to evaluate the benefit on xenobiotic detoxification of larval exposure to HIPVs released by the host plant maize (Zea mays). Larval exposure of the invasive alien species S. frugiperda to maize HIPVs significantly enhanced their tolerance to all three of the well-known defensive compounds 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one (DIMBOA), chlorogenic acid, and tannic acid in maize and the two commonly used insecticides methomyl and chlorpyrifos. HIPV exposure also improved the larval tolerance of S. litura third instars to chlorogenic and tannic acids. Furthermore, larval exposure to either maize HIPVs or DIMBOA induced the activities of cytochrome P450 enzymes (P450s), glutathione-s-transferase (GST), and carboxylesterase (CarE) in the midguts and fat bodies of the two insects, while the induction was significantly higher by the two components together. In addition, the expression of four genes encoding uridine diphosphate (UDP)-glycosyltransferases (UGT33F28, UGT40L8) and P450s (CYP4d8, CYP4V2) showed similar induction patterns in S. frugiperda. Cis-3-hexen-1-ol, an important component in maize HIPVs, also showed the same functions as maize HIPVs, and its exposure increased larval xenobiotic tolerance and induced the detoxification enzymes and gene expression. Our findings demonstrate that HIPVs released by the pest-infested host plants are conductive to the xenobiotic tolerance of lepidopteran insect larvae. Hijacking the host plant HIPVs is an important strategy of the invasive alien polyphagous lepidopteran pest to counter-defend against the host plant’s chemical defense.
Influence of Fused Deposition Modeling Process Parameters on Constitutive Model of Hyperelastic Thermoplastic Polyurethane
Authors: Gallup Lucas , Trabia Mohamed , O’Toole Brendan , Fahmy Youssef
Published: December 2024

Thermoplastic polyurethanes (TPUs) are suited for fused deposition modeling (FDM) of parts that require high levels of flexibility and strength. Predicting the deformation of TPU parts produced using FDM may be difficult, especially under large deformations, as their constitutive models depend on the printing process parameters. The lack of understanding led to the absence of constitutive models for TPU parts produced using FDM. This work aims to identify accurate hyperelastic constitutive models. Six groups of uniaxial tensile specimens were produced using FDM. These groups were made with variations in two process parameters, which were infill geometry and extrusion nozzle temperature. Infill geometries either corresponded to a zero-deposition angle (wall-only) or an infill deposition of ±45° raster angle (infill-only). It was determined that a third-order Mooney–Rivlin constitutive model can accurately describe these six groups. A finite element analysis (FEA) of the experiments using the proposed constitutive models resulted in limited errors for all groups. The proposed approach was verified through a combination of experiments and FEA of FDM TPU components undergoing large deformation.
Multi-Parametric Optimization of 3D-Printed Components
Authors: Vaida Calin , Pop Grigore , Tucan Paul , Gherman Bogdan , Pisla Doina
Published: December 2024

This study explores the experimental and theoretical optimization of process parameters to improve the quality of 3D-printed parts produced using the Fused Deposition Modeling technique. To ensure the cost-effective production of high-quality components, advancements in printing strategies are essential. This research identifies optimal 3D printing strategies to enhance the quality of finished products. Form and dimensional tolerances were assessed using a 3D Coordinate Measuring Machine, and the resulting data were analyzed via Design Expert software version 9.0.6.2. Design Expert for experimental design was utilized and an Analysis of Variance was conducted to validate the models’ accuracy. The results indicate that a 45° raster angle, combined with internal raster values between 0.5048 and 0.726, minimizes flatness, cylindricity, and dimensional deviations by optimizing deposition patterns and thermal dynamics. Internal raster values below 0.308 resulted in insufficient support and greater deviations, while higher values enhanced stability through improved interlayer adhesion. Experimental validation confirmed these parameter settings as optimal for producing precise and consistent 3D-printed parts.
Active Polymers Decorated with Major Acid Groups for Water Treatment: Potentials and Challenges
Authors: Kumar Avneesh , Chang Dong Wook
Published: December 2024

Polymers exhibiting ion-conduction capabilities are essential components of water-purifying devices. These polymers not only transport selective ions but are also mechanically robust; thus, they can be processed as membranes. In this review, we highlight major acidic polymers and their engineered morphologies and optimized properties, including metal selectivity and water permeation or retention. Crucial phenomena, such as self-assembly in acid-group-functionalized polymers for driving water transportation, are discussed. It was observed that the phosphonic acid groups containing polymers are rather suitable for the selective adsorption of toxic metals, and thus, are superior to their sulfonated counterparts. Additionally, due to their amphoteric nature, phosphonated polymers displayed several modes of metal complexations, which makes them appropriate for eliminating a wide range of metals. Further observation indicates that aromatic-acid-functionalized polymers are more durable. Temperature- and pH-responsive polymers were also found to be promising candidates for a controlled water-treatment process. Nevertheless, considering the morphology, water retention, and metal adsorption, acid-functionalized polymers, especially phosphonated ones, have the potential to remain as the materials of choice after additional advancements. Further perspectives regarding improvements in acidic polymers and their fabricated membranes for water treatment are presented.
Tribological Performance of Short Fibers Reinforced Thermoplastic Polyurethane Composite Materials Under Water-Lubricated Condition
Authors: Yu Yicong , Jiang Pan , Yu Wei , Guo Zhiwei
Published: December 2024

The water-lubricated bearing plays a crucial role in the ship propulsion system, significantly impacting vessel safety. However, under the harsh working conditions of low-speed and heavy-load, the lubrication state of water-lubricated bearings is usually poor, leading to serious friction and wear. To improve the tribological performance of composites and reduce friction, three short fibers (ultra-high-molecular-weight polyethylene fibers, basalt fibers, and bamboo fibers) with the same mass fraction (5%) were added into the melted thermoplastic polyurethane (TPU). The tribological behavior of these three composites under different loads and rotation speeds was investigated using the CBZ-1 friction and wear tester. Through the comprehensive analysis of the friction coefficient, the wear mass loss, and the surface morphology, it was confirmed that the filled fiber positively affected the tribological performance of thermoplastic polyurethane materials. The experimental results indicated that basalt fiber significantly improved the tribological performance of TPU, and the friction coefficient of the sample was only 0.088 under the working conditions of 0.5 MPa and 250 r/min, which was 70.57% lower than that of pure TPU material. And in all the tests, the minimum wear of the basalt fiber-reinforced composite is only 0.4 mg, which is also the smallest of all the materials under all conditions, and a decrease of 98.69% compared to TPU. Under high loads, ultra-high-molecular-weight polyethylene fiber and bamboo fiber-reinforced composites have smoother surfaces and exhibit better tribological properties. This study provides an experimental foundation for tribological performance enhancement for environmentally friendly, water-lubricated bearing composites.
Cardiomyopathy in Non-Ambulatory Patients with Duchenne Muscular Dystrophy: Two Case Reports with Varying Outcomes, Considering Novel Treatments
Authors: Marcì Marcello , Vaccaro Paola , Polizzi Vincenzo , Crescimanno Grazia
Published: December 2024

Background and Clinical Significance: Cardiomyopathy is a significant cause of mortality in patients with Duchenne muscular dystrophy (DMD). Key prognostic factors include the age of onset of cardiomyopathy, low body mass index (BMI), and poor respiratory function. Detection of cardiac abnormalities can be challenging, which complicates timely diagnosis and treatment. Common treatments for heart failure include ACE inhibitors, beta-blockers, and mineralocorticoids. However, their effectiveness can vary, and the progression of cardiomyopathy may differ from one patient to another. Ongoing research aims to identify better therapeutic strategies and biomarkers for early intervention, ultimately improving the quality of life for patients affected by cardiomyopathy. New medications for heart failure, such as sodium/glucose co-transporter 2 inhibitors (SGLT2i) and valsartan/sacubitril (V/S), have been proposed, but their safety and efficacy in DMD patients remain unknown. Case Presentation: We present two cases that illustrate the histories of two patients who experienced different outcomes. The management of the first patient was complicated by several factors, including an early onset of cardiomyopathy, intolerance to ACE inhibitors, and untreated scoliosis, which hindered the implantation of a cardioverter defibrillator (ICD). Unfortunately, he only benefited from dapagliflozin in the later stages of his cardiomyopathy. Neurological complications further exacerbated the advanced state of his disease. In contrast, the second patient adhered to all recommended therapies, including innovative medications, and he currently has compensated heart failure. Conclusions: We concluded that several factors, beyond genetic ones, may have influenced their prognosis, including updated guidelines for cardiomyopathy treatment and the utilization of innovative medications.
Application of Artificial Intelligence in Landslide Susceptibility Assessment: Review of Recent Progress
Authors: Kudaibergenov Muratbek , Nurakynov Serik , Iskakov Berik , Iskaliyeva Gulnara , Maksum Yelaman , Orynbassarova Elmira , Akhmetov Bakytzhan , Sydyk Nurmakhambet
Published: December 2024

In the current work, authors reviewed the latest research results in landslide susceptibility mapping (LSM) using artificial intelligence (AI) methods. Based on an overall review of collected publications, the review was classified into four sections based on their complexity: single-model approaches, enhanced models with optimization, ensemble models, and hybrid models. Each category offers distinct advantages and is suited to specific geographic and data conditions, enabling the selection of an optimal model type based on the complexity and requirements of the mapping task. Among models, random forest (RF), support vector machine (SVM), convolutional neural network (CNN), and multilayer perception (MLP) are used as the baseline to compare any new model introduced to develop LSM. Moreover, compared to previous review works, the number of LSM conditioning factors used in AI models are significantly increased, up to 122 factors. Their relation to the AI models is illustrated using Sankey diagram, while a radar chart is used to further visualize the dataset size per reviewed work for comparative purposes. In the main part of the current review work, the main findings are summarized into a table form, where the reader can find the overall relations between landslide conditioning factors, landslide dataset size, applied AI models, and their accuracy on predicting LSM for selected geographical locations. In terms of the regions, Asia is leading in the application of AI models to generate LSM, and in such regions with dense populations falling into higher landslide risk categories, there are more ongoing research activities, using modern AI methods. This trend underscores the increased use of AI in disaster management, with implications for improving practical applications, such as early warning systems and informing policy decisions aimed at risk reduction in vulnerable areas.
Characterizing Changes in Geometry and Flow Speeds of Land- and Lake-Terminating Glaciers at the Headwaters of Yarlung Zangbo River, Western Himalayas
Authors: Zhou Min , Wang Yuzhe , Zhang Tong , Sun Weijun , Wang Yetang
Published: December 2024

The glaciers of the Himalayas are essential for water resources in South Asia and the Qinghai–Tibet Plateau, but they are undergoing accelerated mass loss, posing risks to water security and increasing glacial hazards. This study examines long-term changes in the geometry and flow speeds of both land- and lake-terminating glaciers at the headwaters of the Yarlung Zangbo River, using field measurements, remote sensing, and numerical ice flow modeling. We observed significant heterogeneity in glacier behaviors across the region, with notable differences between glacier terminus types and even among neighboring glaciers of the same type. Between 1974 and 2020, glacier thinning and mass loss rates doubled in the early 21st century (−0.57±0.05 m w.e. a−1) compared to 1974–2000 (−0.24±0.11 m w.e. a−1). While lake-terminating glaciers generally experienced more rapid retreat and mass loss, the land-terminating N241 Glacier displayed comparable mass loss rates. Lake-terminating glaciers retreated by over 1000 m between 1990 and 2019, while land-terminating glaciers retreated by less than 750 m. The ITS_LIVE velocity dataset showed higher and more variable flow speeds in lake-terminating glaciers. Numerical modeling from 2000 to 2017 revealed divergent changes in flow regimes, with lake-terminating glaciers generally experiencing acceleration, while land-terminating glaciers showed either a slowing down or stable flow behavior. Our findings underscore the significant role of lake-terminating glaciers in contributing to ice mass loss, emphasizing the need for advanced glacier models that incorporate dynamic processes such as frontal calving and longitudinal coupling.