bluuebunny/crossref_metadata_2025 huggingface dataset:
MITOCHONDRIAL SIRTUIN 3 (SIRT3) IN AGEING: EXPLORING CELLULAR RESPONSES TO ETOPOSIDE-INDUCED DNA DAMAGE IN MALE AND FEMALE MOUSE EMBRYONIC FIBROBLASTS
Authors: Šimunić Ena , I. Podgorski Iva , Popović Hadžija Marijana , Belužić Robert , Paradžik Mladen , Dončević Lucija , Balog Tihomir , Kaloper Marta , Habisch Hansjörg , Madl Tobias , Korać Aleksandra , Sobočanec Sandra
Published: September 2024

Ageing is a complex process characterised by the gradual deterioration of physiological functions and increased susceptibility to various age-related diseases. Mitochondrial dysfunction is an important factor contributing to ageing. Sirtuin 3 (Sirt3), a mitochondrial protein essential for energy homeostasis, plays a critical role in maintaining mitochondrial function, as loss of Sirt3 reduces energy and impairs cellular repair, which accelerates ageing. The aim of this study was to investigate the role of Sirt3 in male and female mouse embryonic fibroblasts (MEF) exposed to etoposide-induced DNA damage. We employed state-of-the-art genetic, molecular, and imaging technologies as well as metabolomic analyses to provide insights into the molecular mechanisms underlying these responses. We found that the loss of Sirt3 affected metabolic responses differently depending on sex: while male MEF showed minimal damage, possibly due to earlier stress adaptation, female MEF lacking Sirt3 were more vulnerable, suggesting that Sirt3 plays a critical role in enhancing their ability to withstand such challenges. By focusing on Sirt3 and sex-specific signalling pathways it modulates, this study has a potential for developing new strategies to combat diseases associated with DNA damage — a cornerstone of the ageing process.
Factors and Strategies in the Implementation of Flipped Classrooms Among Nursing Students
Authors: Wen Zhou , Young Leslie
Published: September 2024

The flipped classroom represents a progressive pedagogical approach prioritizing active student engagement and participation through pre-class preparation. This study aimed to investigate the factors and strategies influencing the implementation of the flipped classroom model within nursing education reform. Employing a quantitative descriptive design, data were gathered using a survey questionnaire administered to undergraduate nursing interns at a hospital in China, with a sample size of 169 participants selected through simple random sampling. Demographically, the study found that most participants were female, and a significant portion fell within the 20-22 age range. Approximately 47.3% reported having one year or more of experience with the flipped classroom, 37.6% had reliable internet access, and 42% possessed three or more electronic devices. The overall implementation of equipment and resources and teacher and student involvement in the flipped classroom was reported to be high, reflecting robust adoption within the curriculum. Specifically, the study revealed a very high level of implementation of flipped classroom strategies within the context of nursing curriculum reform. Notably, while there was no significant correlation between the implementation of equipment and resources and variables such as gender, age, internet connectivity, or device ownership, there was a significant association with the duration of exposure to the flipped classroom model. Furthermore, the study identified gender-related differences in the strategies employed for flipped classroom implementation. However, no significant disparities were found based on gender, internet connectivity, or the number of digital devices among undergraduate nursing students. However, there was a noteworthy divergence in strategies based on the length of experience with the flipped classroom. Overall, among the key influencing factors—equipment and resources, teachers, and students—teachers emerged as the most influential in shaping the meaningfulness of the flipped classroom experience for nursing trainees. Their pivotal role underscores the importance of educator engagement and facilitation in optimizing the benefits of this innovative educational approach. Keywords: factors, strategies, flipped classroom, nursing students
Socioeconomic inclusion of family farmers from Cooperative of Producers of the Branch of Banco, Amazonas
Authors: Rosário Soares Thalissa Bianca , Souza da Costa Francimara , De Jesus Pinto Fraxe Therezinha , Lima Santiago Jozane , Verona Carvalho Gonçalves Vinicius
Published: September 2024

Despite being a state with a large number of rural producers, in Amazonas, it is common that they do not have institutional assistance in the development of their activities. Faced with this scenario, agricultural cooperatives present themselves as an opportunity to mediate socioeconomic inclusion strategies for rural communities and their farmers. The objective of this work was to promote actions to strengthen the Mixed Cooperative of the Bank’s Branch Producers - COOMPRAB, located in the municipality of Rio Preto da Eva / Amazonas. A training course, a workshop, purchase tests were carried out at the AGROUFAM Fair and participant observation. The results were systematized and the discussion took place based on a bibliographic review. The actions carried out brought technical knowledge, which is considerably scarce in rural communities in Amazonas, so that farmers could use in their work routine alternatives to improve their production, to improve the use of available resources and add value to the products sold. In addition, the actions allowed the board and the members to observe the current scenario of the cooperative’s operation, creating a panorama from which they could identify the points that need to be improved in order to achieve full functioning and the collective objectives, providing better quality of service. life to families in the community.
Prospek Bengkel Sepeda Motor untuk Usaha Kecil dan Menengah (PkM di SMK Dharma Karya Kecamatan Beringin Kabupaten Deli Serdang)
Authors: Ronald Naibaho  , Napitupulu Abidan , Habibullah
Published: September 2024

SMK Dharma Karya yang ada di Desa Sidodadi Kecamatan Beringin Kabupaten Deli Serdang adalah Sekolah Kejuruan tingkat atas yang memiliki Program Studi Teknik Bisnis Sepeda Motor (TBSM).  Dengan  melakukan Pemberdayaan Masyarakat kepada siswa kelas XI melalui dialog, motivasi  dan diskusi terbuka terhadap permasalahan lapangan pekerjaan dan tantangan perekonomian Indonesia umumnya dan khususnya Sumatera Utara yang dihadapi saat ini dan dimasa yang akan datang sesuai dengan peluang alumni SMK Prodi TBSM maka  setelah tamat adik adik siswa SMK Dharma Karya diharapkan tidak hanya untuk bekerja tetapi mau dan berani membuka lapangan kerja dengan mendirikan bengkel sepeda motor. Dengan semakin banyaknya produksi sepeda motor dan semakin murah nya Down payment (DP) atau uang muka untuk membeli sepeda motor maka masyarakat akan semakin terrangsang untuk memiliki sepeda motor yang nantinya tentu akan  semakin membutuhkan jasa service dan penggantian spare part sepeda motor. Adapun tujuan PkM ini adalah untuk memotivasi siswa SMK Dharma Karya Program Studi Teknik Bisnis Sepeda Motor (TBSM) memahami peluang membuka bengkel sepeda motor yang akan semakin menjajnjikan sebagai sebuah usaha kecil dan menengah (UMK) yang modalnya tidak terlalu besar.  Dengan adanya dialog, memberikan motivasi dan diskusi maka pengetahuan siswa bertambah dan semakin termotivasi untuk mendirikan bengkel sepeda motor setelah tamat. Dengan metode pendekatan yang partisipatif dan kolaborasi beberapa siswa ternyata memberikan dampak yang positif. Hal ini terlihat dari adanya respon siswa dan komitmen untuk berwiraswata setelah siswa tamat dari SMK Dharma Karya.
Pemasangan Antena Parabola Dan Perlengkapan Penerima Siaran Televisi Dari Satelit Untuk Tiga Televisi Sebagai Upaya Meningkatkan Penyebaran Informasi Pada Desa Salak I Kecamatan Salak Kabupaten Pakpak Bharat Propinsi Sumatera Utara
Authors: Banurea Waldemar , Junaidi  , Hutajulu Elferida , Sirait Regina
Published: September 2024

Masyarakat Desa Salak I Kecamatan Salak Kabupaten Pakpak Bharat Propinsi Sumatera Utara tidak dapat siaran televise secara maksimal, agar dapat menerima siaran televisi biasanya pesawat televisi dilengkapi perlengkapan komunikasi wireless, salah satu perlengkapan komunikasi wireless adalah antena. Desa mereka jauh dari repeater televisi teresterial sehingga mereka tidak dapat menggunakan jenis antena untuk komunikasi televisi teresterial seperti antena Yagi-Uda, untuk mengatasi masalah tersebut maka dirancang dan direalisasikan Sistem Penerima Siaran Televisi dari Satelit yang dapat menjawab kebutuhan masyarakat tersebut sehingga masyarakat dapat mendengarkan dan menonton siaran televisi sehingga masyarakat dapat meningkatkan penguasaan informasi untuk memungkinkan meningkatkan taraf ekonominya maupun meningkatkan kesejahteraan masing-masing. Umumnya satu set Antena Parabola/LNB untuk satu set Digital Satellite Receiver/Televisi, akan tetapi setelah mengaplikasikan perlengkapan Multiswitch maka satu set Antena Parabola/LNB dapat digunakan untuk tiga set Digital Satellite Receiver/Televisi dengan siaran yang dapat berbeda-beda sesuai dengan keinginan user tersebut. Antena parabola dan perlengkapan penerima siaran televisi dari Satelit dipasang di Rumah Warga atau di Kedai/Warung dan disediakan fasilitas penyambungan untuk dua Digital Satellite Receiver/Televisi lagi, sehingga satu set antena parabola dan perlengkapan penerima siaran televisi dari Satelit tersebut dapat digunakan untuk tiga set Digital Satellite Receiver/Televisi.
Pelatihan Thermo Control Heater Bagi Teknisi Pabrik Thermo Forming
Authors: Fitriani Ayu , Syahputra Syofyan Anwar , Panjaitan Joel , Pakpahan Arnold , Fadlan Siregar Muhammad , Sirait Regina , Harahap Muhcsin
Published: September 2024

Perusahaan manufaktur plastik yang menggunakan mesin thermo-forming banyak berkembang dengan seiring jaman. Pabrik ini memproduksi bahan material plastik menjadi barang jadi berbentuk gelas cup plastik. Heater digunakan untuk proses pemanasan material plastik untuk dicetak menjadi berbentuk gelas. Proses pengontrolan heater sangat berpengaruh terhadap kualitas hasil produksi. Untuk mengontrol proses heater diperlukan Thermo Control dan Solid State Relay untuk control temperatur heater sesuai dengan yang diinginkan. Pemahaman tentang operasional dan perbaikan thermo control heater ini sangat penting bagi para teknisi yang bekerja diperusahaan tersebut. Atas dasar ini maka dilakukan pengabdian untuk pelatihan para teknisi pabrik.
Pengenalan Pajak dan Pelatihan Perhitungan Pajak Penghasilan (PPh) untuk Para Petani di Desa Pardugul Lumban Binanga Kabupaten Samosir
Authors: Sinurat Windo , Silitonga Manaor , Gultom Josua Alex , Sitohang Andar
Published: September 2024

Pajak merupakan salah satu penerimaan Negara terbesar. Penerimaan Negara menentukan pembangunan di seluruh pelosok negeri Indonesia. Menurut undang-undang pajak sifat nya memaksa tetapi sistem Perpajakan Indonesia adalah Self assessment yaitu wajib pajak akan berinisiatif dalam kegiatan menghitung dan memungut pajaknya sendiri. Akan tetapi masih banyak masyarakat Indonesia yang tidak paham akan pajak terkhususnya pajak penghasilan. Maka dengan ini kami pihak dosen dari Teknik Akadeni Deli Serdang Lubuk Pakam melakukan pengabdian kepada masyarakat (PKM) ini dalam rangka menjalankan slaah satu Darma dari Tri Darma Perguruan Tinggi. Dengan melakukan sosialisi ini tentu akan berdampak baik bagi masyarakat dan Negara. Adapun tujuan dari pengabdian ini adalah dengan semakin banyak nya masyarakat yang paham akan pajak maka di harapkan akan semakin tinggi juga kesadaran masyarakat dalam hal penyetoran pajak ke Negara. Hal ini secara tidak langsung akan mengakibatkan semakin banyak nya pembangunan fasilitas-fasilitas untuk membantu masyarakat juga. Dengan adanya dialog dengan masyarat diharapkan dapat memotivasi masyarakat untuk sadar akan pajak. Pengabdian  kepada masyarakat (PKM) ini di sambut hangat oleh masyarakat Desa Pardugul, Lumban Binanga Kabupaten Samosir.
Stress Factors of Health Care Environment: Concern for Health Care Workers
Authors: Tarafdar M A
Published: September 2024

Occupational stress has been a long-standing concern of the health care industry. Occupational stress and workplace health have become issues of great concern over the last decade, both internationally and nationally. The management of job-related stress among health-care workers is critical for the improvement of healthcare services Stress is a pervasive and insidious part of everyday life and in the work environment.
Dengue Crisis : A New Challenge for Bangladesh
Authors: Huda SMN
Published: September 2024

Dengue, a viral infection spread by the infamous Aedesaegypti mosquito, is the fastest growing mosquito-bornedisease in the world today. It is now a major internatioinalpublic health concerns. Over the past three decades therehas been dramatic global increase in the frequency ofdengue fever, dengue haemorhagic fever and dengue shocksyndrome and their epidemics. It is found in tropical andsubtropical regions around the world. Some 2.5 billionpeople i.e two fifth of the world's population in tropical andsubtropical countries are at risk of the disease. Anestimated 50 million dengue infection occurs worldwideannually and about 500,000 people with denguehaemorrhagic fever require hospitalization each year.Approximately 90 per cent of them are children aged lessthan five years, and about 2.5 per cent of those affected die.During epidemics, infection rate among those who havenot been previously exposed to the virus are often 40 to 50per cent, but can also reach 80 to 90 per cent.
Gastroduodenal Intussusception of a Gastrointestinal Stromal Tumour
Authors: Rahman MM
Published: September 2024

Gatruduodenal Intussception is a very rare clinical entity reported in the literature. This is a rare case in a 8 year old boy who presented with features of recurrent fever, vomiting and Jaundice. A gastroduodenal intussusception of a large gastric stromal tumour, which presented with intermittent abdominal pain and gastric outlet obstruction. Pre-operative diagnosis was made on abdominal ultrasound and confirmed at laparotomy and histopathological examination.
A Study on Environmental Sanitation in Some Selected Villages of Bhanga, Faridpur
Authors: Rahman M T
Published: September 2024

This descriptive type of cross sectional study was conducted during the period of September 2017-February2018 to assess thestatus of environmental sanitation and the level of literacy and practices of hygiene of rural mothers in selected villages ofBhanga, Faridpur. Sample size was 190 and the respondents were rural mothers. Data were collected from 190 rural mothersthrough convenient sampling. After taking verbal consent, a face to face interview was conducted through a pre-tested semi-structural questionnaire. The study revealed that majority of the respondents (56.32%) was illiterate. The study showed that allmost all (97.36%) respondents used tube well water for drinking purpose. The study also showed that majority (91.57%) hadknowledge about safe water. It was also revealed from the study that majority of the respondents had the idea about water bornediseases caused by consumption of unsafe water. Majority 73.68% respondents lived in Kacha house and only 18.95%respondents had sanitary latrines. It is concluded from the study that there is inadequate knowledge about environmentalsanitation particularly housing and disposal of human excreta and also the rate of illiteracy is significantly high amongstrespondents.
Operative outcomes of Laparoscopic Cholecystectomy
Authors: Rahman MM
Published: September 2024

Background: Laparoscopic cholecystectomy is the most commonly performed operation of the digestive tract. It is considered as the gold standard treatment for cholelithiasis. Aim: To evaluate the outcome of it regarding length of hospital stay, complications, morbidity and mortality at a secondary hospital.

Materials and Methods: Data ware collected by retrospective record review method from 200 patients who underwent laparoscopic cholecystectomy. Patients' demographics, co-morbid diseases, previous abdominal surgery, conversion to open cholecystectomy, operative time, postoperative complications, and hospital stay were collected and analyzed.

Results: Out of 200 patients, 165 (82.5%) were females and 35 (17.5%) males. The mean age of the patients was 49.35±8.68 years. Mean operative time was 65.94±11.52 min. Five cases (2.5%) were converted to open surgery, one due to obscure anatomy (0.5%) 2 due to acute cholecystitis and oedematous gall bladder (1.0%) and one by bleeding (0.5%) one for bile duct injury. Two (1.0%) cases had biliary leakage. Mean hospital stay was 2.6±1.5 days. Sixteen (8.0%) developed wound infection. There was no case of port site hernia, bowel injury or spilled gallstones. There was no mortality recorded in this series.

Conclusions: Laparoscopic cholecystectomy is a safe and effective line for management of gallstone disease that can beperformed with acceptable morbidity at a secondary hospital.
Public Health Issues in Bangladesh: Concern for Everyone
Authors: Tarafdar M A
Published: September 2024

Bangladesh faces major public health challenges.Communicable diseases, childhood malnutrition, Unsafefood by chemical substances and toxins contaminationremain major threat to public health in Bangladesh.Bangladesh remains among the countries with highest TBburden in the world. Pneumonia and infectious diseases arestill concern for young children. Drug abuse becomingserious threat to public health in Bangladesh day by day, non-communicable diseases like cancer, diabetes, cardiovasculardiseases, and chronic respiratory diseases have an upwardtrend for the couple of decades in Bangladesh.

Changing lifestyles, persistent social inequality, unstable social life and economic insecurity are posing new health challenges of non-communicable diseases, substance abuse, and mental health problems
Study on Status of Blood Pressure among Adults in Selected Villages of Modhukhali
Authors: Zaman F
Published: September 2024

Introduction: Hypertension (HTN) is the most common cardiovascular ailment, a very important preventable cause of cerebro-vascular accidents (CVAs) and coronary heart diseases (CHDs). Hypertension is classically an “iceberg disease”-even in most developed countries, only half of the hypertensive persons are aware of their condition.

Objective: Objective of the study was 'To assess the status of blood pressure among the adults of selected villages of Modhukhali. Methodology: It was a descriptive cross sectional study conducted for the period of six months from October, 2018 to March, 2019. Purposive sampling technique was used to collect 371 samples. The data were collected by face to face interview by a pretested questionnaire from the selected villages of Modhukhali. Then data were analyzed according to the objectives.

Results: The study reveals that most (65.5%) of the respondents were female and majority (25.61%) of the respondents belong to 30 – 39 years age group. Among the respondents most (39.35%) of the family had monthly income Tk.10001– Tk.20000. The study also reveals that 21.56% respondents' knew that they were hypertensive; around 7.20% were newly diagnosed case of HTN. About 34.21% had optimal range of BP, 27.22% were within normal range, 8.60% had high normal blood pressure and 1.20% had isolated systolic hypertension. Majority (62.52%) of the high blood pressure was diagnosed by MBBS doctor and about 72.5% respondents were taking medicine for high blood pressure. Regarding factors associated to HTN, about 37.47% were tobacco user and among the all tobacco users 59.0% were taking smokeless tobacco, 20.86% were taking smoked tobacco and 20.14% were both smokeless and smoked tobacco user. About 47.43% took extra salt in meal and 39.35% do not perform physical activity regularly.

Conclusion: From this study it is concluded that HTN is one of the major non-communicable disease among rural people about which more measures & appropriate action should be taken.
Clinical and Medical Imaging Findings of the Forefoot Following a COVID-19 Infection: A Case Report
Authors: Casciato Dominick , Thomas Randall , Houser Joshua
Published: September 2024

Since the beginning of the SARS-CoV-2 pandemic, manifestations of the COVID-19 virus have been reported throughout the body, visible both clinically and radiographically. In the foot and ankle, one such phenomenon presents as cutaneous changes associated with neuralgia to the digits. Although rare, emerging reports described similar manifestations of COVID-19 in the foot, though limited to superficial structures. In this case report, we present a 52-year-old male experiencing burning pain and hyperpigmented lesions to the digits of his left foot 2 weeks prior to a formal diagnosis with COVID-19. Advanced imaging showed a third interspace neuroma as well as distal-to-proximal bone marrow edema in the distal phalanx of all digits, a pattern seen in vasculopathies, 4 weeks after diagnosis. In the absence of diabetes mellitus or peripheral vascular disease, the patient underwent a simple neurectomy. At the follow-up appointment 12 weeks after the initial encounter, his symptoms resolved. Our study reports a case describing osseous along with cutaneous manifestations in the foot of a patient with COVID-19.
Walking Bike as an Effective Tool to Reduce Plantar Peak Pressure in Diabetes Mellitus
Authors: Illgner Ulrich , Mehlhorn Alexander , Osada Nani , Krenn Veit , Landauer Franz
Published: September 2024

Background: Foot ulcers and infections are a major and costly problem in patients with diabetes and a major cause of amputations. Plantar peak pressure plays an essential role in plantar ulceration. Off-loading is a common tool to reduce plantar peak pressure and risk of ulceration. The goal of this study was to determine whether reduction of plantar peak pressure can be achieved using a walking bike (a bike without pedals) compared with walking.

Methods: The study starts with a PubMed literature review. In a blinded prospective protocol, 14 healthy individuals (seven men, seven women; mean ± SD age, 39.5 ± 11.3 years) are included. In-shoe pedobarography sensors were attached between the skin and the standardized shoes, then participants walked 10 m three times and then moved over the same distance using a walking bike without removal of the sensor (three times) in a gait laboratory (84 measurements).

Results: In this single-blinded prospective study, mean ± SD plantar peak pressure was significantly reduced from 49.4 ± 12.9 N/cm2 with walking to 35.2 ± 14.6 N/cm2 using a walking bike (P = .003). Mean ± SD step length increased significantly from 0.68 ± 0.13 m to 0.91 ± 0.19 m (P < .001) due to a significantly reduced number of steps (from 7.7 ± 1.4 steps per 10 m of walking to 5.7 ± 1.1 steps per 10 m of using a walking bike; P < .001).

Conclusions: Plantar peak pressure is a risk factor for ulceration in diabetes. Herein, a significant reduction of plantar peak pressure was seen using a walking bike compared with walking (P = .003). Walking bikes may be a tool for off-loading for diabetic patients, especially if both feet are ulcerated. Additional studies to validate these findings in patient care are planned.
The effect of combining antibiotics on resistance: A systematic review and meta-analysis
Authors: Siedentop Berit , Kachalov Viacheslav N , Witzany Christopher , Egger Matthias , Kouyos Roger D , Bonhoeffer Sebastian
Published: September 2024

When and under which conditions antibiotic combination therapy decelerates rather than accelerates resistance evolution is not well understood. We examined the effect of combining antibiotics on within-patient resistance development across various bacterial pathogens and antibiotics.We searched CENTRAL, EMBASE and PubMed for (quasi)-randomised controlled trials (RCTs) published from database inception to November 24
th
, 2022. Trials comparing antibiotic treatments with different numbers of antibiotics were included. A patient was considered to have acquired resistance if, at the follow-up culture, a resistant bacterium (as defined by the study authors) was detected that had not been present in the baseline culture. We combined results using a random effects model and performed meta-regression and stratified analyses. The trials’ risk of bias was assessed with the Cochrane tool.42 trials were eligible and 29, including 5054 patients, were qualified for statistical analysis. In most trials, resistance development was not the primary outcome and studies lacked power. The combined odds ratio (OR) for the acquisition of resistance comparing the group with the higher number of antibiotics with the comparison group was 1.23 (95% CI 0.68-2.25), with substantial between-study heterogeneity (

I
2

=77%). We identified tentative evidence for potential beneficial or detrimental effects of antibiotic combination therapy for specific pathogens or medical conditions.The evidence for combining a higher number of antibiotics compared to fewer from RCTs is scarce and overall, is compatible with both benefit or harm. Trials powered to detect differences in resistance development or well-designed observational studies are required to clarify the impact of combination therapy on resistance.
Characterization of cancer-driving nucleotides (CDNs) across genes, cancer types, and patients
Authors: Zhang Lingjie , Deng Tong , Liufu Zhongqi , Chen Xiangnyu , Wu Shijie , Liu Xueyu , Shi Changhao , Chen Bingjie , Hu Zheng , Cai Qichun , Liu Chenli , Li Mengfeng , Tracy Miles E , Lu Xuemei , Wu Chung-I , Wen Hai-Jun
Published: September 2024

A central goal of cancer genomics is to identify, in each patient, all the cancer-driving mutations. Among them, point mutations are referred to as cancer-driving nucleotides (CDNs), which recur in cancers. The companion study shows that the probability of i recurrent hits in n patients would decrease exponentially with i; hence, any mutation with i ≥ 3 hits in The Cancer Genome Atlas (TCGA) database is a high-probability CDN. This study characterizes the 50–150 CDNs identifiable for each cancer type of TCGA (while anticipating 10 times more undiscovered ones) as follows: (i) CDNs tend to code for amino acids of divergent chemical properties. (ii) At the genic level, far more CDNs (more than fivefold) fall on noncanonical than canonical cancer-driving genes (CDGs). Most undiscovered CDNs are expected to be on unknown CDGs. (iii) CDNs tend to be more widely shared among cancer types than canonical CDGs, mainly because of the higher resolution at the nucleotide than the whole-gene level. (iv) Most important, among the 50–100 coding region mutations carried by a cancer patient, 5–8 CDNs are expected but only 0–2 CDNs have been identified at present. This low level of identification has hampered functional test and gene-targeted therapy. We show that, by expanding the sample size to 105, most CDNs can be identified. Full CDN identification will then facilitate the design of patient-specific targeting against multiple CDN-harboring genes.
Identificación de infección por el virus de la inmunodeficiencia humana en dos receptores de órgano sólido tres años después del trasplante
Authors: Mopán Nancy Carolina , Plazas Diana Carolina , Salinas María Angélica , Arias-Murillo Yasmín Rocío , Cortés Jorge Alberto
Published: September 2024

El examen de rutina de los donantes de órganos para detectar la infección por el virus de la inmunodeficiencia humana (HIV) ha hecho que la transmisión del virus mediante el trasplante de órganos sea poco común. Sin embargo, a pesar de las pruebas de detección de rutina, la transmisión del HIV continúa siendo un riesgo del trasplante de órganos ya que, a diferencia de los tejidos, los órganos sólidos no se pueden procesar, desinfectar, ni modificar para inactivar patógenos infecciosos.A continuación, se describe un caso de posible transmisión de HIV por trasplante de órganos de un donante previamente seronegativo a dos de sus receptores.
First identification of Angiostrongylus spp. in Lissachatina fulica and Cornu aspersum in Antioquia, Colombia
Authors: Gamarra-Rueda Ramón , García Ricardo , Restrepo-Rodas Diana C. , Pérez-García Janeth
Published: September 2024

Introduction. Abdominal and neural angiostrongyliasis caused by Angiostrongylus costaricensis and A. cantonensis, respectively, are zoonotic diseases involving snailsas intermediate hosts. Colombia has already reported human cases, and the increasing distribution of Lissachatina fulica and Cornu aspersum raises public health concerns due to the potential risk of disease transmission in areas where parasites and hosts coexist. Objective. To identify the presence of Angiostrongylus spp. in snail species L. fulica and C. aspersum in Antioquia, Colombia.Materials and methods. This prospective cross-sectional study had a population of 5,855 L. fulica and C. aspersum snails captured in the ten towns of the Valle de Aburrá (Antioquia, Colombia), 169 samples were collected in 28 sampling points. Lung tissues of the collected snails were dissected and analyzed to detect Angiostrongylus spp. through molecular techniques.Results. Angiostrongylus spp. were identified in both L. fulica and C. aspersum. Angiostrongylus costaricensis was detected in 18 pooled prevalence of 30% (95% CI =19.2-43.3), and Medellín was the municipality with the highest number of positive samples (33.3%). Seventy-two-point-two percent of the positive places reported the presence of rodents. None of the tests were positive for A. cantonensis.Conclusion. Our findings provide important insights into the epidemiology and distribution of Angiostrongylus spp. in Antioquia, Colombia. The identification of these parasitic nematodes in L. fulica and C. aspersum highlights the potential role of these snails as intermediate hosts in the transmission of Angiostrongylus spp. infections in the Valle de Aburrá, with implications for human and veterinary health.
Effect of combined application of inorganic nitrogen and phosphorus to an organic-matter poor soil on soil organic matter cycling
Authors: Anwar Faiza , Sanaullah Muhammad , Ali Hayssam M. , Hussain Sabir , Mahmood Faisal , Zahid Zubda , Shahzad Tanvir
Published: September 2024

Background

Sequestering carbon dioxide (CO2) in agricultural soils promises climate change mitigation as well as sustainable ecosystem services. In order to stabilize crop residues as soil carbon (C), addition of mineral nutrients in excess to crop needs is suggested as an inevitable practice. However, the effect of two macronutrients i.e., nitrogen (N) & phosphorus (P), on C cycling has been found contradictory. Mineral N usually decreases whereas mineral P increases the soil organic C (SOC) mineralization and microbial biomass. How the addition of these macronutrients in inorganic form to an organic-matter poor soil affect C cycling remains to be investigated.

Methods

To reconcile this contradiction, we tested the effect of mineral N (120 kg N ha−1) and/or P (60 kg N ha−1) in presence or absence of maize litter (1 g C kg−1 soil) on C cycling in an organic-matter poor soil (0.87% SOC) in a laboratory incubation. Soil respiration was measured periodically during the incubation whereas various soil variables were measured at the end of the incubation.

Results

Contrary to literature, P addition stimulated soil C mineralization very briefly at start of incubation period and released similar total cumulative CO2-C as in control soil. We attributed this to low organic C content of the soil as P addition could desorb very low amounts of labile C for microbial use. Adding N with litter built up the largest microbial biomass (144% higher) without inducing any further increase in CO2-C release compared to litter only addition. However, adding P with litter did not induce any increase in microbial biomass. Co-application of inorganic N and P significantly increased C mineralization in presence (19% with respect to only litter amended) as well as absence (41% with respect to control soil) of litter. Overall, our study indicates that the combined application of inorganic N and P stabilizes added organic matter while depletes the already unamended soil.
Comparison of soil quality assessment methods for different vegetation eco-restoration techniques at engineering disturbed areas
Authors: Zhao Bingqin , Gao Ruzhang , Zhang Xingfeng , Xia Lu , Zhang Lun , Xia Dong , Liu Daxiang , Xia Zhenyao , Xu Wennian
Published: September 2024

Scientific assessment of soil quality is the foundation of sustainable vegetation eco-restoration in engineering disturbed areas. This study aimed to find a qualitative and comprehensive method for assessing soil quality after vegetation eco-restoration in engineering disturbed areas. Sixteen soil indicators were used at six vegetation eco-restoration sites as the potential soil indicators. A minimum data set (MDS) and revised minimum data set (RMDS) were determined by principal component analysis. Six soil quality indices (SQIs) of varying scoring functions based on different data sets were employed in this study. Significant positive correlations were observed among all six SQIs, indicating that the effects of different vegetation eco-restoration measures on soil quality could be quantified by all six SQIs. The SQI values of the vegetation concrete eco-restoration slope (VC), frame beam filling soil slope (FB), thick layer base material spraying slope (TB), and external-soil spray seeding slope (SS) were all significantly higher than the SQI value of the abandoned slag slope (AS). It is noteworthy that the SQIs of the VC and TB sites were also significantly higher than the SQI of the natural forest (NF) site. These results indicate that the application of artificial remediation measures can significantly improve the soil quality of the disturbed area at the Xiangjiaba hydropower station. The results of this study also indicate that the SQI-NLRM method is a practical and accurate quantitative tool for soil quality assessment and is recommended for evaluating soil quality under various vegetation eco-restoration techniques in disturbance areas at the Xiangjiaba hydropower station and in other areas with similar habitat characteristics.
Effect of Cooking Conditions on Cooking Yield, Juiciness, Instrumental and Sensory Texture Properties of Chicken Breast Meat
Authors: Alugwu Samson Ugochukwu. , Okonkwo Thomas M. , Ngadi Michael O
Published: September 2024

The aim of the research was to investigate the effect of baking and grilling temperatures and times on quality characteristics of chicken breast meat. Eight packs of Industrial skinless chicken breast meat samples were purchased, frozen and sliced into dimensions, thawed and cooked by baking (BK) and grilling (GR) at 170, 180 and 190 oC for 0, 4, 8, 12 and 16 min. The cooking yield and loss were assessed by weight changes before and after cooking, juiciness, instrumental and sensory texture changes were investigated using 3 x 2 x 5 factorial experiment in complete randomized design (CRD). Relationships between changes in instrumental and sensory textures were evaluated. The cooking yield decreased significantly (p <0.05) and ranged from 67.99% to 70.90%, while cooking losses increased significantly (p <0.05) and ranged from 28.71% to 31.48%. Cooking decreased significantly (p < 0.05) mechanical properties of juiciness from 41.65% to 24.53%, but increased significantly (p < 0.05) hardness of chicken breast meat from 6.79 N to 11.33 N. The sensory texture showed that samples cooked by BK had higher textural score of 4.72 than GR with 4.52, but both cooking methods were rated neither crispy nor soft by the panelists. It was found that instrumental texture correlated positively with sensory texture in cooked chicken breast meat while sensory texture reversed with instrumental texture with respect to cooking parameters. Considering the overall quality indices evaluated, baking is the best cooking method at 170 oC for 8 min.
An Economic Analysis of Production and Marketing of Banana in Middle Gujarat
Authors: Pundir R. S. , Vahoniya D. R. , Singh Ritambhra , Rajwadi Alvira
Published: September 2024

India is the second largest producer of fruits in the world (horticultural statistics at a glance 2018) and area, production and productivity of banana has increased over the years in Gujarat as well as in middle Gujarat also. In middle Gujarat, banana is one of the major fruit crop which is economically viable to the farmers as it gives higher return, so there is need to study the economics of fruit cultivation. Different cost for banana cultivation was calculated using cost concept. Different marketing channel was identified and marketing efficiency was calculated using Acharys’s formula. The study revealed that the cost A was ₹ 278397.31 per ha and cost C2 was ₹ 396162.82 per ha for banana cultivation and benefit cost ratio over cost C2 was 2.06. There were three marketing channels identified in middle Gujarat region. Marketing efficiency was higher in channel IV, which was 1.23. Unavailability of labour, increasing prices of inputs and unavailability on time of inputs were the major production constraints faced by farmers. Not getting proper price for produce, unavailability of labour and higher commission charges were the major marketing constraints faced by farmers. They suggest to focus on to strengthen supply chain through formation of FPOs, so the problems related to inputs and output prices can be solved. And with the help of new mechanical advancement problem of post-harvest losses and labour shortage can be solved.
Genotypic Variation in Plant Height, Branching, and Yield Components of Field Pea: Implications for Crop Improvement
Authors: Tawarkhed Sachin , VH Ashvathama , R Navyashree , Patil MD , UV Mummigatti
Published: September 2024

Field pea (Pisum sativum L.) is an essential legume crop with significant nutritional value, but its cultivation faces challenges such as reduced light levels and variable yields. This study aimed to evaluate the morphological, phenological, and yield parameters of different field pea genotypes to identify high-performing varieties. Experiment was conducted using a randomized complete block design (RCBD), the experiment assessed plant height, number of primary branches, flowering and maturity timings, dry matter partitioning, and yield components. Significant differences were observed among genotypes in plant height, branching, and various yield parameters. The genotype IC381455 exhibited superior performance with the highest values for total dry matter, pod yield per plant, seed yield per hectare, and test weight. It also showed higher leaf, stem, and pod dry weights at multiple growth stages. In contrast, Nippani local-2 consistently recorded the lowest values across these parameters. The highest harvest index was recorded for IPF4-9, indicating effective biomass conversion to seed. The results highlight the genetic variability among genotypes and underscore the importance of selecting high-performing varieties to improve yield and crop quality.
Evaluation of the Combining Ability in Mulberry (Morus spp.)
Authors: K. Ramya Harika , Mangammal P. , Devanand P S , Tejasree P. , Garai Ishita , B Karthick Mani Bharathi , Kiruba M. , Kumar P.
Published: September 2024

The research aimed to comprehend the patterns of gene action influencing the inheritance and expression of quantitative features in mulberry by studying combing ability. Four parents viz., V1, G4, MR2 and S36 were used in mating design. Estimates of SCA depicted that crosses V1 × G4 (shoot diameter and chlorophyll - b) and it’s reciprocal cross G4 × V1 (chlorophyll – a, total chlorophyll) showed high significant SCA effects. V1 × MR2 for shoot diameter and lowest positive SCA for internodal distance. Overall, the crosses with the highest SCA values for specific traits consistently involved V1 as one of the parents. This observation highlights that V1, which possesses the best general combining ability (GCA) effect, contributes significantly to the high SCA observed in these crosses for the traits studied. V1 and MR2 were the best general combiners, with high GCA for growth traits. Crosses G4×V1 and V1×G4 demonstrated positive SCA effects for most traits followed by S36 × V1 and MR2 × V1. Notably, SCA variance was greater than GCA variance for most traits, indicating the dominance of non-additive gene effects.
Heat Stress and Thermoregulation in Insects: Unveiling Molecular and Physiological Responses in Light of Climate Change
Authors: Kumar Hemant , Sagar Doddachowdappa , Verma Sweta , Rajarushi C. N. , J. S. Rupali , Madhuri E. Vidya , Godavari  , J. S. Pavan , Chandel Anamika , G. S. Sujatha
Published: September 2024

Insects being poikilotherms rely on external temperatures to regulate their body heat that making them vulnerable to climate change as temperature fluctuations directly impact their growth, reproduction and survival as well as control their metabolic and physiological processes. Many insect species are in danger as global temperatures are increasing since they cannot physiologically adapt themselves for long exposure to high temperatures. This vulnerability is especially pronounced in species that have not evolved mechanisms to endure extreme heat, leading to potential population decline and disruptions in ecosystems Understanding the mechanisms of how insects manage heat stress is crucial for predicting their behaviour and biology in the scenario of a changing climate. This review delves into the genetic basis of thermoregulation in insects, exploring how genes influence their ability to cope with heat stress and regulate their internal body temperature in hot conditions through mechanisms such as heat shock proteins, metabolic adjustments, and behavioural changes. It also highlights gaps in current research and suggests future studies, emphasizing the need for comprehensive investigations into the molecular, genetic, and ecological aspects of thermoregulation. Advanced insights into insect thermoregulation will enhance our understanding of insect population dynamics and facilitate the development of effective pest management strategies, contributing to climate-resilient agriculture amidst the challenges posed by a changing climate.
Weed Management for Enhancing the Growth Attributes and Yield of Pearl Millet
Authors: Sivanantha J. , Manimaran S. , Sudhakar P. , Venkatakrishnan D.
Published: September 2024

The field experiment was conducted in the Experimental Farm, Department of Agronomy, Faculty of Agriculture, Annamalai University, Annamalai Nagar, Tamil Nadu during June - September, 2023 to study the weed management for enhancing growth attributes and yield of pearl millet. There are eleven weed management treatments viz., T1 - Atrazine @ 0.5 kg a.i. ha-1 (Pre), T2 - Pendimethalin @ 0.75 kg a.i. ha-1 (Pre), T3 - Pretilachlor @ 0.45 kg a.i. ha-1 (Pre), T4 - Atrazine @ 0.5 kg a.i. ha-1 (Pre) + 2, 4-D @ 0.5 kg a.i. ha-1 (PoE), T5 - Pendimethalin @ 0.75 kg a.i. ha-1 (Pre) + 2, 4-D @ 0.5 kg a.i. ha-1 (PoE), T6 - Pretilachlor @ 0.45 kg a.i. ha-1 (Pre) + 2, 4-D @ 0.5 kg a.i. ha-1 (PoE), T7 - Atrazine @ 0.5 kg a.i. ha-1 (Pre) + Tembotrione @ 0.08 kg a.i. ha-1 (PoE), T8 - Pendimethalin @ 0.75 kg a.i. ha-1 (Pre) + Tembotrione @ 0.08 kg a.i. ha-1 (PoE), T9 - Pretilachlor @ 0.45 kg a.i. ha-1 (Pre) + Tembotrione @ 0.08 kg a.i. ha-1 (PoE), T10 - Two hand weeding at 15 and 30 DAS and T11 - Unweeded Control were laid out in randomized block design with three replications. Among the herbicide treatments, Pendimethalin @ 0.75 kg a.i. ha-1 (Pre) + Tembotrione @ 0.08 kg a.i. ha-1 (PoE) (T8) recorded the higher growth attributes and yield of pearl millet viz., plant height (155.84 cm), leaf area index (4.97) and dry matter production (7822 kg ha-1), grain yield (3347 kg ha-1) and stover yield (5155 kg ha-1). The unweeded control (T11) recorded the lowest values of growth attributes, grain and stover yield of pearl millet.
Beneficial Role of Soil Microbiome in Enhancing Crop Productivity, Insight from Recent Study
Authors: Kumar Mukul , Kumari Niru , Pandey Amit Kumar , Singh Ashutosh
Published: September 2024

Reducing the use of synthetic fertilizers and pesticides is the goal of current agricultural practices, which calls for low-input technology to improve food production sustainability and restore ecosystem function. The geochemical cycles and plants function rely heavily on the contributions of the soil biota to many ecosystem endeavours. As a result of interactions between host plants roots and microbes in the rhizosphere, these beneficial microorganisms perform a variety of functions that promote plants growth, such as fixing, mineralizing, solubilizing, and mobilizing nutrients; producing siderophores, antagonistic substances, and antibiotics; and releasing hormones that promote plants growth, such as auxin and gibberellin. Microbes that are vital for agriculture and possess properties that may dissolve iron and zinc can be employed to biofortify micronutrients in various cereal crops. Our capacity to manage soil fertility and create a high-yield food production system will be sustained by our increased understanding of plants-microbe interactions in both natural and agroecosystems. Utilising the microbiome effectively contributes to a safe environment, which benefits human health. In an effort to increase soil productivity and fertility for future generations, soil microbiologists are working to analyse the diversity of soil niches and then try to characterise the roles of these soil residents at trophic levels.
Pendampingan Komunitas dalam Melakukan Upaya Peningkatan Imun Tubuh di Kelurahan Jeruk Bangkingan dan Panti Asuhan Kasih Agape
Authors: Djoar Raditya Kurniawan , Anggarani Anastasia Putu Martha
Published: September 2024

Kegiatan pengabdian masyarakat ini bertujuan untuk mendampingi komunitas dalam upaya meningkatkan imun tubuh di Kelurahan Jeruk Bangkingan dan Panti Asuhan Kasih Agape selama masa pandemi. Pandemi telah menekankan pentingnya menjaga kesehatan, terutama dengan memperkuat daya tahan tubuh sebagai langkah pencegahan terhadap infeksi. Peningkatan imun tubuh menjadi penting untuk menjaga kesehatan dan mencegah penyebaran penyakit. Program ini dilakukan dengan melibatkan serangkaian pendidikan kesehatan yang mencakup cara-cara alami dan praktis untuk meningkatkan daya tahan tubuh, seperti penerapan pola makan sehat, olahraga teratur, manajemen stres, dan kebiasaan hidup bersih. Metode yang digunakan meliputi penyuluhan dan demonstrasi, diskusi interaktif, dan pendampingan langsung dalam penerapan materi yang diajarkan. Sebelum intervensi, tingkat pengetahuan responden mengenai cara meningkatkan imun tubuh masih rendah, sebanyak 17,3% responden yang memiliki pengetahuan yang baik. Setelah kegiatan pendampingan dan pendidikan kesehatan dilakukan, terjadi peningkatan signifikan dalam pengetahuan responden, dengan jumlah 80,4% responden memiliki tingkat pengetahuan yang baik. Peningkatan ini mencerminkan efektivitas program dalam meningkatkan kesadaran dan pemahaman masyarakat mengenai pentingnya menjaga kesehatan melalui peningkatan imun tubuh. Selain itu, kegiatan ini juga menggarisbawahi pentingnya intervensi yang berkelanjutan dan berbasis komunitas dalam menangani tantangan kesehatan di masa pandemi. Dengan demikian, pendampingan komunitas ini berhasil memperkuat ketahanan kesehatan masyarakat dan mendorong perubahan perilaku positif yang berkelanjutan.