bluuebunny/crossref_metadata_2025 huggingface dataset:
Long‐term comparison of high‐ and low‐dose oral immunotherapy in children with anaphylactic cow's milk allergy
Authors: Ito Yu , Nagakura Ken‐ichi , Sato Sakura , Ebisawa Motohiro , Yanagida Noriyuki
Published: February 2025

Background

Long‐term evidence on maintenance doses of oral immunotherapy (OIT) for anaphylactic cow's milk allergy is insufficient.

Methods

We retrospectively compared the three‐year safety, efficacy, and adherence between OIT with a maintenance dose of 200 mL of cow's milk (HOIT, 2009–2013) and 3 mL of cow's milk (LOIT, 2013–2019). Patients aged 6–18 years with a history of anaphylaxis reacting to ≤3 mL of cow's milk during oral food challenge (OFC) were included. Adverse symptoms, OFC negative rate after 2 weeks of avoidance, dropout rate, and immunological changes were compared.

Results

The median ages in the HOIT (n = 78) and LOIT (n = 99) groups were 8.1 and 7.8 years, with milk‐specific IgE levels of 56.5 and 49.2 kUA/L, respectively. The percentages of doses triggering symptoms were 20.88%, 13.73%, and 7.31% in the HOIT group and 11.81%, 8.15%, and 6.30% in the LOIT group during years 1, 2, and 3, respectively. After 3 years, 29% of patients in the HOIT group passed the OFC with 200 mL, and 47%, 18%, and 5% of patients in the LOIT group passed the OFC with ≥25 mL, ≥50 mL, and 100 mL of cow's milk, respectively. After 3 years, the dropout rates were 24% and 11% in the HOIT and LOIT groups and milk‐specific IgE levels decreased by 88% and 78% in the HOIT and LOIT groups, respectively.

Conclusion

HOIT enables higher dose consumptions. LOIT might be safer and have higher adherence in patients with anaphylactic cow's milk allergy.
The impact of the European Federation of Periodontology on European and global periodontology
Authors: Sanz Mariano , Kamma Joanna , Goldstein Moshe , Tonetti Maurizo
Published: February 2025

The European Federation of Periodontology (EFP), founded in 1991, is a non‐profit organization comprising 43 national periodontal societies worldwide, representing over 18 000 professionals. Its mission is to promote periodontology and oral health globally. The EFP's strategic objectives include advancing the practice and development of periodontology, promoting scientific research, ensuring the integrity of the profession, and raising awareness of the link between periodontal and general health. These goals are pursued through initiatives such as EuroPerio, the EFP's flagship scientific congress held every 3 years, which attracts thousands of global participants, and European Workshops in Periodontology (EWPs), which foster evidence‐based discussions and produce consensus reports. These workshops have produced landmark publications, including the new classifications of periodontal diseases and S3‐level clinical practice guidelines for the treatment of periodontitis and peri‐implant diseases. Additionally, the organization plays a central role in setting standards for postgraduate education in periodontology, accrediting programs across Europe and internationally, with 24 universities offering EFP‐accredited programs as of 2024. The EFP also publishes the Journal of Clinical Periodontology, a key platform for disseminating scientific and clinical research. Through outreach campaigns in collaboration with global partners, the EFP aims to raise awareness of the links between periodontal health and systemic diseases, engaging medical professionals, international organizations, policymakers, and patient groups. A notable initiative is Gum Health Day, held annually on May 12, to promote public awareness of periodontal health. The EFP's activities have significantly contributed to the development of modern periodontology, both academically and clinically, with a substantial global impact.
On the properties of distance covariance for categorical data: Robustness, sure screening, and approximate null distributions
Authors: Zhang Qingyang
Published: February 2025

Pearson's Chi‐squared test, though widely used for detecting association between categorical variables, exhibits low statistical power in large sparse contingency tables. To address this limitation, two novel permutation tests have been recently developed: The distance covariance permutation test and the U‐statistic permutation test. Both leverage the distance covariance functional but employ different estimators. In this work, we explore key statistical properties of the distance covariance for categorical variables. Firstly, we show that, unlike Chi‐squared, the distance covariance functional is B‐robust for any number of categories (fixed or diverging). Second, we establish the strong consistency of distance covariance screening under mild conditions, and simulations confirm its advantage over Chi‐squared screening, especially for large sparse tables. We illustrate this novel screening method using the General Social Survey data. Finally, we derive an approximate null distribution for a bias‐corrected distance correlation estimate, demonstrating its effectiveness through simulations and real‐world applications.
Topology Optimization-Driven Design of Container Ship Hatch Cover for Lightweight Performance and Cost Efficiency
Authors: Yilmaz Ahmet Fatih , Konal Mehmet
Published: January 2025

Recent economic conditions and technological developments in manufacturing methods have necessitated the use of advanced engineering design and analysis methods, such as topology optimization to increase efficiency and reduce costs in structural designs in the shipbuilding industry, as in many manufacturing sectors. Finite element method-based topology optimization aims to optimize a lightweight design without compromising the desired strength in ship structural designs, thus increasing efficiency in postmanufacturing functionality. Thus, it helps to create innovative products that can meet the sustainable design and manufacturing needs in the shipbuilding industry. In this study, the design of a foldable hatch cover for a feeder container ship was improved using topology optimization. Based on the premise geometry obtained by topology optimization, the model design was updated and an optimum design with the desired strength at minimum mass was obtained through iterative analyses. The optimization study resulted in a weight reduction of 2.49 tons and a material cost saving of $3.635 for a hatch cover. Thus, a total saving of $50.890 is expected from raw materials, labor, and consumables for 14 hatch covers on a sample ship. In addition, due to reduced draft and consequently lower drag, a 6-kW reduction in main engine power will result in approximately 6.6 tons less annual fuel consumption. Furthermore, an 8-mm vertical reduction in the ship's center of gravity increases stability by approximately 0.6% according to International Maritime Organization (IMO) stability criteria.
Magnetorheological response of homogeneous and Janus iron-oxide coated silica particles
Authors: Habibi Samin , Bryant Steven L. , Shor Roman J. , Natale Giovanniantonio
Published: January 2025

The flow behavior of magnetic suspensions made of nonmagnetic spherical particles surface-decorated by a magnetic shell is investigated in this study. Suspensions of SiO2@Fe3O4 homogeneous particles (HPs) (the so-called core/shell particles) and SiO2@Fe3O4 Janus particles (JPs) were compared in terms of their rheological properties. Particle surface characteristics and the contribution of anisotropic magnetization of JPs to interparticle interactions were investigated for the first time. Higher shear viscosity, shear stress, and viscoelastic modulus were obtained in magnetic HP suspensions. However, upon elimination of the saturation magnetization effect of particles, higher shear viscosity was achieved in magnetic JP suspensions due to the more significant contribution of the contact force in the JP systems. The dependence of the magnetorheological (MR) properties on the magnetic particle concentration and magnetic field strength was also evaluated for HP and JP suspensions. These two magnetic systems deviate from conventional MR fluids because the magnetization is generated by the magnetic shell instead of the core of the particles. These observations provide new insights and opportunities for designing MR fluids.
A more rapid method for transformation of
Helicobacter pylori
Authors: Skene Caroline D. , Ferrero Richard L.
Published: January 2025

Helicobacter pylori
is a major causative agent in several upper gastroduodenal tract diseases, including gastric cancer. The development of methods to genetically manipulate
H. pylori
by natural transformation has allowed a greater understanding of its biology and role in these diseases. Nevertheless, the transformation methods used for
H. pylori
are time-consuming, requiring growth of these fastidious and slow-growing bacteria from −80°C stocks. The aim of the study was to develop a more rapid and convenient method for generating
H. pylori
mutants. We describe here a method in which competent
H. pylori
bacteria can be stored at −80°C and used in transformations on the day of resuscitation, similar to methods routinely used for
Escherichia coli
. This means that transformation can be performed at will and that transformants can be obtained within days, rather than weeks. Furthermore, we show that bacteria remain competent for at least six months storage at −80°C and that the method is applicable to strains with varying levels of natural competence. Transformation efficiencies of the bacteria varied between 10
1
and 10
6
transformants/total colony-forming units/µg donor DNA, depending on the strain. We suggest that this improved method will facilitate studies on
H. pylori
and, moreover, may be applicable to other naturally transformable pathogens with fastidious growth requirements and requiring ultra-low temperature refrigeration for long-term preservation.

IMPORTANCE

Genetic manipulation is an important tool in the study of pathogenic bacteria and their interactions with the host. Many pathogenic bacteria are naturally transformable; however, transformation experiments can be impeded by the slow-growing and fastidious nature of some species. One such bacterium is
Helicobacter pylori
, which requires resuscitation from −80°C and multiple subcultures prior to transformation. The method described in the current study uses a simple modification of a conventional method of natural transformation. Using this method, competent
H. pylori
bacteria can be stored for long periods (at least six months) and resuscitated as needed for use in experiments. The method circumvents the need for multiple and lengthy subcultures prior to transformation, nor does it involve costly materials, complicated procedures, or sophisticated equipment. Thus, we describe a simple, inexpensive, and time-efficient method that may have broader applications for use with other fastidious bacteria.
Reconstructions of F(𝒫) gravity from barrow holographic dark energy, barrow agegraphic dark energy and new barrow agegraphic dark energy
Authors: Singha Anup Kumar , Sadhukhan Rupali , Debnath Ujjal
Published: January 2025

This paper presents the reconstructed [Formula: see text] cubic gravity from Barrow Holographic Dark Energy (BHDE), Barrow Agegraphic Dark Energy (BADE) and New Barrow Agegraphic Dark Energy (NBADE). By considering the scale factor of the universe as [Formula: see text] and the deformation quantity of the quantum gravity by [Formula: see text], we have reconstructed the [Formula: see text] cubic gravity and then we have analyzed the reconstructed models through cosmic parameters, namely, by deceleration and state parameters taking different values of deformation quantity [Formula: see text]. Also, we have focused on the dark energy perturbation stability through the sound-squared speed parameter. Again, we have projected the evolution of the universe in the plane of [Formula: see text]-[Formula: see text].
Artificial Intelligence in Professional Services: A Systematic Review and Foundational Baseline for Future Research
Authors: Bezuidenhout Carl , Abbas Roba , Mehmet Michael , Heffernan Troy
Published: January 2025

Purpose: The growing body of literature on Professional Services Firms (PSFs) using Artificial Intelligence (AI) offers valuable insights, yet a synthesised, systematic examination of this literature is lacking. This paper addresses this research gap by synthesising the literature at the intersection of AI and regulated PSFs, unveiling key issues, and proposing a research agenda for future studies. Design/Methodology/Approach: Through a systematic literature review conducted in January 2023, we evaluated 612 articles and analysed 75 academic papers in detail, originally published over 34 years between 1988 and 2022. This temporal boundary offers a stable benchmark for future comparative studies, providing context for understanding the impact of even newer and emerging technologies. Findings: Our findings reveal that the use of AI within PSFs has a profound impact, far beyond the initial economic or efficiency gains expected. We uncover five dominant themes within the literature, which include the motivations for using AI, challenges and limitations of AI, human-AI interaction, increasing ethical considerations, and the strategic implications for PSFs. Originality/Value: This paper offers a synthesised, systematic examination of AI in regulated PSFs, which is notably absent in the literature. We present a visual summary of the themes and overall findings, illustrating the complex interplay of AI within PSFs. We propose a research agenda of future research opportunities emerging from the research.
Elevated Frame Rates During Exercise Echocardiography Improve Speckle Tracking Success Rate and Augment Deformation Values
Authors: Spahiu Fabian , Mook Michelle , Helbig Lars C. , Stöhr Eric J.
Published: January 2025

Background: Although 2D speckle-tracking echocardiography (STE) is important for the clinical quantification of myocardial function it remains unknown whether increased frame rates during exercise STE augment tracking success and absolute deformation values.

Method: 19 participants (15 male and 4 female; age 26.7±4.8) underwent step-wise exercise testing on a recumbent bicycle. Exercise started at 50 W, increasing by 30 W every 3 min until a target heart rate of 130–140 bpm was reached. During last 90 seconds of each exercise stage, echocardiographic sequences for offline quantification of longitudinal strain (LS), peak twist, untwisting velocity, basal rotation and apical rotation were acquired with high (HFPS), medium (MFPS), and low frame rates (LFPS). Differences in tracking success were determined using chi-square test and the impact of different frames rates on absolute deformation values were compared using mixed-model-analysis.

Results: Utilization of HFPS significantly improved tracking success for parasternal short-axis images. LS acquired at HFPS was highest at baseline and across all exercise stages. Similar trends were observed for twist, peak untwisting velocity and apical rotation, while basal rotation showed no differences. Mixed model analysis revealed a significant effect of frame rate setting on LS (p<0.05) and untwisting velocity (p<0.05).

Conclusion: In contrast to recommendations by leading organizations advocating for frame rates between 40 and 80 fps during resting conditions, with a proportional increase as heart rate rises, our findings suggest that consistently maintaining the frame rate at the highest feasible level is preferable for achieving optimal tracking success and accuracy in STE.
Experimental and Simulation Study of High‐Efficiency Heat Storage Materials for Aerospace Applications
Authors: Geng Hui , Rao Peng
Published: February 2025

To meet the stringent requirements of high heat transfer performance and lightweight nature for aerospace heat storage equipment (HSE), this paper incorporates fins into the phase change material (PCM) and conducts an optimization design through experiment and simulation. Utilizing n‐eicosane for heat storage and aluminum alloy 6061 as the fin material, the objective is to devise an HSE for high‐power heating elements, ensuring that the hot end temperature remains below 50°C and the weight is within 3.5 kg during the operational period. Heat sources of 50 and 100 W are, respectively, employed for experimental testing. The experiments reveal that when the heating power is 100 W, the temperature in the heat concentration region ascends to 43.2°C after a heating duration of 1900 s; in the scenario where the heating power is 50 W, the maximum temperature reaches 38.0°C after 3800 s of heating. The discrepancy between the simulation results and the experimental outcome is within 5%, thereby attesting to the relatively high accuracy of the simulation. Simulations were carried out with the thickness and number of fins as variables to analyze the heat transfer performance and weight of the HSE. The results show that within the research range, as the fin thickness increases, the overall heat transfer effect is enhanced. When the fin thickness increases at equal intervals between 1.0 and 3.0 mm, except for 3.0 mm, the hot end temperature of the HSE gradually decreases, and the weight also increases with the increase of the fin thickness. With the increase in the number of fins, the hot end temperature decreases, and the decrease in the hot end temperature is particularly significant at 13 fins. Considering the comprehensive influence of heat transfer performance, hot end temperature, and weight in this HSE, the structure with a fin thickness of 2.0 mm and 13 fins is the best. The volume ratio of the PCM in the HSE is between 0.55 and 0.60. According to the optimization results, an HSE with a total weight of 3.33 kg is processed. After using the optimized HSE with a heating power of 50 W continuously applied for 10,000 s, the hot end temperature is 42.17°C, which is 6.9°C lower than that of the HSE without fins, and the melting rate is increased by 46%. After a heating power of 100 W is continuously applied for 4000 s, the hot end temperature is 48.16°C, which is 13.2°C lower than that of the HSE without fins, and the melting rate is increased by 71%. In addition, the heat transfer performance of the HSE has been analyzed, and the change law of the heat transfer performance during the melting process of the PCM was found. This study will provide guiding ideas and reference significance for the research and development of enhancing heat transfer and lightweight of high‐power HSE in aerospace.
Advances in Simulation of Stratigraphic‐Structural Evolution of Basin Fill: A Retrospective to Guide Future Progress
Authors: Dhote Prashant , Bhan Uday
Published: February 2025

The oil and gas industry relies heavily on inverse geostatistical modeling to predict static reservoir properties that influence hydrocarbon accumulation and flow. However, these methods face significant challenges due to sparse sampling and the inability to capture reservoir variability beyond boreholes. Geostatistical techniques typically depend on borehole data, which represent only a small fraction of the total reservoir volume. The large distances between boreholes further hinder the ability to achieve reliable and accurate predictions. An innovative approach in numerical forward modeling, the stratigraphic‐structural forward modeling (SSFM) technique, offers an alternative or complementary workflow for modeling facies and property distribution in static reservoir models. The SSFM quantitatively integrates sedimentation and deformation processes in basins, grounded in the physics of basin formation, infill, and sedimentary architecture. By translating conceptual geological models into cellular geological volumes, SSFM requires minimal borehole and seismic data for validation. This review traces the historical evolution of various numerical techniques, with particular emphasis on the advancements and limitations of SSFM. However, these limitations present opportunities for guiding future research, fostering development in the field, and extending the application of SSFM techniques beyond hydrocarbon exploration. Understanding and addressing SSFM’s limitations is essential to optimizing and enhancing its effectiveness within the industry.
Evaluating the Incidence of Seizures and Its Relationship With Blood Glucose Levels in Tramadol Poisoning Patients Admitted to Imam Reza Hospital in Mashhad (Iran) From 2019 to 2020
Authors: Mousavi Seyed Reza , Jafari Faezeh , Ghamsari Anahita Alizade , AkbariRad Mina , Khadem-Rezaiyan Majid , Mousavi Seyed Amirhossein , Rafati Sadaf Sadat
Published: February 2025

Background: Drug poisoning is the most common type of poisoning in the world. The utilization of tramadol for the management of pain has been identified as a significant contributor to the incidence of poisoning cases. Tramadol poisoning can result in a range of neurological complications, including seizures and a decreased level of consciousness. Tramadol‐induced seizures are frequently dose independent and manifest as generalized tonic‐clonic seizures. The neurotoxic effects of tramadol are primarily manifested within the initial 24 h period following ingestion, with 84.6% of the seizures occurring within the first six hours. In addition, it has been documented that 15%–35% of the patients with tramadol poisoning have experienced seizures. The present study was undertaken to elucidate the clinical and paraclinical signs and symptoms observed in patients with tramadol poisoning and their correlation with the occurrence of seizures.

Methods and Materials: All patients hospitalized due to tramadol poisoning from October 2019 to September 2020 in the poisoning department of Imam Reza Hospital if they met the inclusion criteria were studied. The patients were divided into two groups with and without seizures. The occurrence of seizures was substantiated through the documentation of EMS personnel and the direct observation of the attending physician in the emergency room. Following admission, the patient’s blood glucose level was quantified via a glucometer. A blood sample was also obtained for subsequent laboratory evaluation. In the event of any aberrations in blood glucose levels, a re‐evaluation was conducted at one‐hour intervals using a glucometer. All findings were analyzed using SPSS Version 25 statistical software. The level of significance was set at p < 0.05.

Results: A total of 163 patients were included in this study. In 94 patients (57.3%), some degree of consciousness loss and seizures occurred in 69 patients (42.1%). There was a significant relationship between the occurrence of seizures and the increase in blood glucose levels of patients (p = 0.031). The findings indicated that 60% of the patients with blood glucose levels exceeding 140 mg/dL experienced seizures.

Conclusion: Seizures in tramadol poisoning may be related to the patient’s blood glucose levels.
Supplementary Table S1 from Conditional Activation of c-MYC in Distinct Catecholaminergic Cells Drives Development of Neuroblastoma or Somatostatinoma
Authors: Wang Tingting , Liu Lingling , Fang Jie , Jin Hongjian , Natarajan Sivaraman , Sheppard Heather , Lu Meifen , Turner Gregory , Confer Thomas , Johnson Melissa , Steinberg Jeffrey , Ha Larry , Yadak Nour , Jain Richa , Picketts David J. , Ma Xiaotu , Murphy Andrew , Davidoff Andrew M. , Glazer Evan S. , Easton John , Chen Xiang , Wang Ruoning , Yang Jun
Published: February 2025

CSTN tumor metadata
Supp Figure 7 from Mutant PP2A Induces IGFBP2 Secretion to Promote Development of High-Grade Uterine Cancer
Authors: Haanen Terrance J. , Boock Sophie , Callahan Catherine G. , Peris Irene , Zawacki Kaitlin P. , Raines Brynne , Nino Charles A. , Tran Brian , Harold Alexis , Hodges Onishi Gabrielle , Hinderman Matthew , Dowdican Amanda , Huang Wei , Taylor Derek J. , Taylor Sarah E. , Jackson Mark W. , DiFeo Analisa , O’Connor Caitlin M. , Narla Goutham
Published: February 2025

Supp Figure 7
Figure 2 from Modeling Drug Responses and Evolutionary Dynamics Using Patient-Derived Xenografts Reveals Precision Medicine Strategies for Triple-Negative Breast Cancer
Authors: Shea Abigail , Eyal-Lubling Yaniv , Guerrero-Romero Daniel , Manzano Garcia Raquel , Greenwood Wendy , O’Reilly Martin , Georgopoulou Dimitra , Callari Maurizio , Lerda Giulia , Wix Sophia , Giovannetti Agnese , Masina Riccardo , Esmaeilishirazifard Elham , Cope Wei , Martin Alistair G. , Nagano Ai , Young Lisa , Kupczak Steven , Cheng Yi , Bardwell Helen , Provenzano Elena , Kane Justine , Lay Jonny , Grybowicz Louise , McAdam Karen , Caldas Carlos , Abraham Jean , Rueda Oscar M. , Bruna Alejandra
Published: February 2025

A coclinical trial framework reveals concordant patient–PDTX drug responses. A, Experimental framework (consisting of two trial designs) and associated analytical approach, with modeling metrics used to assess drug response. B and C, TV growth curves displaying linear mixed model fits of trial designs 1 (B) and 2 (C) over treatment duration. Treatment arm for each PDTX model corresponds to the clinical treatment of the matched patient. D and E, Analytical metrics derived from mathematical modeling (as in A). Change in growth rate (top) and estimated difference in the AUC (bottom) for trial design 1 (D) and growth rate under treatment (top) and predicted volume at treatment end (bottom) for trial design 2 (E). F, Box plots displaying growth rate under treatment (top) and predicted volume at treatment end (bottom) for trial design 2 between pCR and non-pCR models. Statistical significance is calculated using the Wilcoxon test.
Table S6 from Kinome-Focused CRISPR-Cas9 Screens in African Ancestry Patient-Derived Breast Cancer Organoids Identify Essential Kinases and Synergy of EGFR and FGFR1 Inhibition
Authors: Madorsky Rowdo Florencia P. , Martini Rachel , Ackermann Sarah E. , Tang Colin P. , Tranquille Marvel , Irizarry Adriana , Us Ilkay , Alawa Omar , Moyer Jenna E. , Sigouros Michael , Nguyen John , Al Assaad Majd , Cheng Esther , Ginter Paula S. , Manohar Jyothi , Stonaker Brian , Boateng Richard , Oppong Joseph K. , Adjei Ernest K. , Awuah Baffour , Kyei Ishmael , Aitpillah Francis S. , Adinku Michael O. , Ankomah Kwasi , Osei-Bonsu Ernest B. , Gyan Kofi K. , Hoda Syed , Newman Lisa , Mosquera Juan Miguel , Sboner Andrea , Elemento Olivier , Dow Lukas E. , Davis Melissa B. , Martin M. Laura
Published: February 2025

List of raw gRNA counts
Data from Ferroptotic Neutrophils Induce Immunosuppression and Chemoresistance in Breast Cancer
Authors: Zeng Wenfeng , Zhang Ruihua , Huang Penghan , Chen Minxia , Chen Houying , Zeng Xin , Liu Jiang , Zhang Jiahui , Huang Di , Lao Liyan
Published: February 2025

Abstract
Inducing ferroptosis in tumor cells is emerging as a strategy for treating malignancies that are refractory to traditional treatment modalities. However, the consequences of ferroptosis of immune cells in the tumor microenvironment need to be better understood in order to realize the potential of this approach. In this study, we discovered that neutrophils in chemoresistant breast cancer are highly sensitive to ferroptosis. Reduction of the acyltransferase MOAT1 in chemoresistance-associated neutrophils induced phospholipid reprogramming, switching the preference from monounsaturated fatty acids to polyunsaturated fatty acids, which increased their susceptibility to ferroptosis. Ferroptotic neutrophils secreted PGE2, IDO, and oxidized lipids that suppressed the proliferation and cytotoxicity of antitumor CD8+ T cells. Furthermore, neutrophil ferroptosis was closely related to a distinct subset of IL1β+CXCL3+CD4+ (Fer-CD4) T lymphocytes, which were enriched in chemoresistant tumors. Fer-CD4 T cells orchestrated neutrophil ferroptosis by modulating MOAT1 expression via IL1β/IL1R1/NF-κB signaling. Moreover, Fer-CD4 T cells secreted CXCL3, IL8, and S100A9 to replenish the neutrophil pool in the tumor microenvironment. Ferroptotic neutrophils in turn fostered Fer-CD4 T-cell differentiation. In spontaneous tumorigenesis mouse models, targeting IL1β+ CD4+ T cells or IL1R1+ neutrophils broke the cross-talk, restraining neutrophil ferroptosis, enhancing antitumor immunity, and overcoming chemoresistance. Overall, these findings uncover the role of neutrophil ferroptosis in shaping the immune landscape and propose appealing targets for restoring immunosurveillance and chemosensitivity in breast cancer.

Significance: In chemoresistant breast cancer, IL1β+CXCL3+CD4+ T cells mediate neutrophil ferroptosis that suppresses antitumor immunity, indicating that interfering with this intercellular cross-talk could be an attractive strategy to reverse chemoresistance.
Data from Fungal Influences on Cancer Initiation, Progression, and Response to Treatment
Authors: Bilal Hazrat , Khan Muhammad Nadeen , Khan Sabir , Shafiq Muhammad , Fang Wenjie , Zeng Yuebin , Guo Yangzhong , Li Xiaohui , Zhao Bing , Lv Qiao-Li , Xu Bin
Published: February 2025

Abstract
Fungal dysbiosis is increasingly recognized as a key factor in cancer, influencing tumor initiation, progression, and treatment outcomes. This review explores the role of fungi in carcinogenesis, with a focus on mechanisms such as immunomodulation, inflammation induction, tumor microenvironment remodeling, and interkingdom interactions. Fungal metabolites are involved in oncogenesis, and antifungals can interact with anticancer drugs, including eliciting potential adverse effects and influencing immune responses. Furthermore, mycobiota profiles have potential as diagnostic and prognostic biomarkers, emphasizing their clinical relevance. The interplay between fungi and cancer therapies can affect drug resistance, therapeutic efficacy, and risk of invasive fungal infections associated with targeted therapies. Finally, emerging strategies for modulating mycobiota in cancer care are promising approaches to improve patient outcomes.
Corticosteroid-Dependent Association between Prognostic Peripheral Blood Cell-Free DNA Levels and Neutrophil-Mediated NETosis in Patients with Glioblastoma
Authors: Till Jacob E. , Seewald Nicholas J. , Yazdani Zachariya , Wang Zhuoyang , Ballinger Dominique , Samberg Heather , Dandu Siri , Macia Camilla , Yin Melinda , Abdalla Aseel , Prior Timothy , Shah Shivani S. , Patel Thara , McCoy Emily , Mansour Maikel , Wills Carson A. , Bochenek Veronica , Serrano Jonathan , Snuderl Matija , Phillips Richard E. , O’Rourke Donald M. , Amankulor Nduka M. , Nabavizadeh Ali , Desai Arati S. , Gollomp Kandace , Binder Zev A. , Zhou Wanding , Bagley Stephen J. , Carpenter Erica L.
Published: January 2025

Purpose:

Noninvasive prognostic biomarkers to inform clinical decision-making are an urgent unmet need for the management of patients with glioblastoma (GBM). We previously showed that higher circulating cell-free DNA (ccfDNA) concentration is associated with worse survival in GBM. However, the biology underlying this is unknown.

Experimental Design:

We prospectively enrolled 129 patients with treatment-naïve GBM with blood drawn prior to initial resection (baseline) and at the time of the first postradiotherapy MRI. We performed ccfDNA methylation deconvolution to determine cellular sources of ccfDNA. ELISA was performed to detect citrullinated histone 3 (citH3), a marker of neutrophil extracellular traps (NET). Multiplex proteomic analysis was used to measure soluble inflammatory proteins.

Results:

We found that neutrophils contributed the highest proportion of prognostic ccfDNA. The percentage of ccfDNA derived from neutrophils was correlated with total [ccfDNA] but only in patients receiving preoperative corticosteroids. At baseline and on therapy, [citH3] was significantly higher in the plasma of patients with GBM receiving corticosteroids compared with corticosteroid-naïve GBM or no-cancer controls. Unsupervised hierarchical clustering of ccfDNA methylation patterns yielded two clusters, with one enriched for patients with the NETosis phenotype and who received corticosteroids. Unsupervised clustering of circulating inflammatory proteins yielded similar results.

Conclusions:

These data suggest neutrophil-mediated NETosis is the dominant source of prognostic ccfDNA in patients with GBM and may be associated with glucocorticoid exposure. If further studies show that pharmacological inhibition of NETosis can mitigate the deleterious effects of corticosteroids, these plasma markers will have important clinical utility as noninvasive correlative biomarkers.
Figure 4 from Targeting Pancreatic Cancer Cell Stemness by Blocking Fibronectin-Binding Integrins on Cancer-Associated Fibroblasts
Authors: Wu Chengsheng , Von Schalscha Tami , Sansanwal Diva , Qian Chen , Jiang Qinlin , Shepard Ryan M. , Wettersten Hiromi I. , McCormack Stephen J. , Weis Sara M. , Cheresh David A.
Published: January 2025

Dual blockade of integrins αvβ3/α5β1 can prevent and reverse CAF-ECM assembly. A, Function-blocking antibodies targeting FN receptors prevent CAF assembly of FN/COL fibers. CAF-1299 cells were incubated with control IgG vs. indicated antibodies for 72 hours and then processed for immunostaining to examine FN and COL. Images are representative of at least three independent experiments. The graph shows the quantification of staining as mean ± SD for each marker that was measured as % area for each experiment and then normalized to IgG control. *, P < 0.05 using one-sample t test. B, Integrin-targeted antibody can disrupt preexisting CAF-produced ECM. CAF-1299 were plated and allowed to produce ECM for 72 hours before adding control IgG vs. indicated antibodies for an additional 72 hours. Samples were then processed for immunostaining to examine FN and COL. The graph shows the mean ± SD staining for each marker measured as % area in each experiment and normalized to IgG control. *, P < 0.05 using the one-sample t test. C, BsAb prevents upregulation of connective tissue growth factor (CTGF), a protein that regulates cell proliferation, migration, and adhesion. CAF-1299 cells were plated and allowed to produce ECM for 72 hours and then treated with antibody for another 72 hours before cells were removed to leave behind a cell-free ECM atop which PANC1 cells were then plated. After 24 hours, the PANC1 cells were lysed and prepared for immunoblotting to detect the protein expression of CTGF. Blots are representative of at least three independent experiments. Tx, treatment.
A quasi-historical approach to the natural history of the differentiation of reptiles in tropical geographic isolates
Authors: Vanzolini P. E.
Published: January 2025

.
Uma nova espécie de Cynolebias do estado do Espírito Santo, Brasil (Pisces, Cyprinodontidae)
Authors: Cruz Carlos Alberto Gonçalves da
Published: January 2025

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Qual é o papel das universidades para as memórias do mundo?
Authors: Muniz Claudia Andreoli
Published: January 2025

Report on the 1st Ibero-American Meeting on University Heritage, held in May 2024 at the University of Seville and organized within the framework of the Ibero-American University Association for Postgraduate Studies (AUIP). The event brought together representatives of different universities and cultural institutions from Spain, Portugal and Latin American countries to share practices, plans and actions regarding the management and protection of university heritage, as well as references that mark their trajectories in terms of cultural heritage and its social importance.
Fragmentos de uma aventura sob ocultação: Sergio Lima e o Surrealismo
Authors: Gonçalves Junior Elvio Fernandes
Published: January 2025

Este trabalho tem como objetivo apresentar alguns aspectos da poesia do surrealista brasileiro Sergio Lima, principalmente a partir de suas duas obras iniciais: Cantos à Mulher Nocturna e Amore. Nossa intenção é, portanto, expor dados relevantes da atuação de Sergio Lima no âmbito do surrealismo. Para isso, recorreremos a alguns dados biográficos e bibliográficos, como as cartas que Sergio enviou a André Breton na década de 1960 e que permitiram sua recepção pelos surrealistas franceses. Além disso, abordaremos fatos concernentes à recepção de sua obra pelos surrealistas portugueses, como Mário Cesariny e Ernesto Sampaio. Ao final, de modo a contribuir com futuros estudos, pretendemos ter criado um perfil deste autor ainda pouco lido e conhecido pelo público em geral.
Manuel de Castro, crítico do Modernismo e do Surrealismo
Authors: Sousa Rui
Published: January 2025

Manuel de Castro é essencialmente conhecido pelo seu trabalho poético, coligido em alguns livros raros mas decisivos. O universo poético do autor é um dos mas originais e representativos do seu tempo, explorando alguns dos mais relevantes motivos poéticos associados ao Grupo do Gafé Gelo. Contudo, Castro foi também um prolífero crítico literário, com textos dispersos em vários jornais portugueses dos anos 60 e 70. Neste txt, proponho uma leitura panorâmica dos textos do autor, tendo como principal tópico de reflexão a relação entre a sua poética e a reflexão em torno do Modernismo e do Surrealismo, sobretudo no contexto da literatura e cultura portuguesas.
Preservation of Vascular Endothelial Function in Late-Onset Postmenopausal Women
Authors: Darvish Sanna , Murray Kevin O. , Ludwig Katelyn R. , Avalani Krisha H. , Craighead Daniel H. , Freeberg Kaitlin A. , Bevers Shaun , Reisz Julie A. , D’Alessandro Angelo , Moreau Kerrie L. , Seals Douglas R. , Rossman Matthew J.
Published: January 2025

BACKGROUND:

Postmenopausal women (PMW) who complete menopause at a late age (55+ years) have lower cardiovascular disease risk than PMW who complete menopause at a normal age (45–54 years). However, the influence of late-onset menopause on vascular endothelial dysfunction is unknown. Moreover, the mechanisms by which a later age at menopause may modulate endothelial function remain to be determined.

METHODS:

We measured endothelial function (brachial artery flow-mediated dilation [FMD
BA
]) in age-matched late- and normal-onset PMW and a young premenopausal reference group. We determined mitochondrial reactive oxygen species (mitoROS)–related suppression of endothelial function (change in FMD
BA
with an acute dose of the mitochondria-targeted antioxidant MitoQ; ΔFMD
BA, MTQ
) in PMW. The effects of serum from late- and normal-onset PMW and premenopausal women on mitoROS bioactivity in human aortic endothelial cells in culture were assessed. Metabolomics analyses in combination with serum metabolite level normalization and human aortic endothelial cell serum exposure experiments were performed to identify the circulating factors contributing to the serum effects on endothelial cell mitoROS bioactivity.

RESULTS:

FMD
BA
in PMW was lower than in premenopausal women. However, FMD
BA
was >50% higher in late- versus normal-onset PMW and positively related to age at menopause. ΔFMD
BA, MTQ
was >50% lower in late- versus normal-onset PMW. Serum from normal-onset PMW but not late-onset PMW induced higher mitoROS bioactivity in human aortic endothelial cells compared with serum from premenopausal women. MitoROS bioactivity was negatively related to FMD
BA
and age at menopause. Seventeen metabolites significantly differed between late- and normal-onset PMW; 15 were lipid specific; 8 were triglyceride derived. TG(16:0) was most strongly correlated with mitoROS bioactivity. Normalization of TG(16:0) concentrations in serum from premenopausal women and late-onset PMW to match serum levels in normal-onset PMW abrogated differences in mitoROS bioactivity in serum-treated human aortic endothelial cells.

CONCLUSIONS:

Late-onset menopause is associated with preservation of endothelial function, which is mediated by lower mitoROS-associated oxidative stress. A more favorable profile of circulating lipid metabolites, specifically triglyceride-derived metabolites, contributes to lower endothelial cell mitoROS in late-onset PMW. These findings provide new insight into the possible mechanisms of reduced cardiovascular disease risk in late-onset menopause.
Categorization and discrimination of human and non-human primate affective vocalizations: Investigation of frontal cortex activity through fNIRS
Authors: Debracque Coralie , Ceravolo Leonardo , Clay Zanna , Grandjean Didier , Gruber Thibaud
Published: January 2025

Previous research has highlighted the involvement of frontal regions in human participants while they engaged in the explicit decoding, such as categorization (A vs B) and discrimination (A vs non-A), of affective signals. Given its adaptive value and deep evolutionary history, this human capacity to recognize the affective content in human calls is likely to extend to the vocalizations of other closely related species, such as non-human primates. However, few comparative studies have thus far investigated this process at both the behavioral and neural levels. Here, we aimed to study the role of frontal regions in human participants while they engaged in the explicit affective content decoding of primate calls using functional Near Infrared Spectroscopy (fNIRS). Specifically, we recorded frontal regions of participants while they categorized or discriminated positive and negatively valenced vocal signals produced by four different primates: humans, chimpanzee and bonobo (both great apes species), and rhesus macaques (a more distant species). We also analyzed whether behavioral responses correlated with recorded frontal activations. fNIRS data revealed more activations within the inferior frontal cortex pars triangularis (IFCtri), the frontopolar (FPC), and middle frontal cortices (MFC) during discrimination compared with categorization. Activity in these regions was modulated by both the species and the type of task, with greater activity during the discrimination of agonistic chimpanzee calls compared with categorization. Categorization was itself characterized by a decrease of frontal activity during the correct recognition of all chimpanzee calls, and of affiliative rhesus macaque and agonistic bonobo vocalizations. Our results also highlighted behavioral differences related to the type of task. Participants discriminated almost all affective cues of all four species vocalizations above chance level. In comparison, they correctly categorized the affective content of most human and great ape vocalizations above chance level, but not those of rhesus macaque calls, highlighting an effect of both phylogenetic relatedness and the type of task. Overall, these findings support the hypothesis of an evolutionary ancient affective recognition processing system situated in the frontal cortex, inherited from our last common ancestor with other great apes.
Use of Statistical and Acoustic Cues for Speech Segmentation in French-Learning 7-Month-Old Infants and French-Speaking Adults
Authors: Marimon Mireia , Berdasco-Muñoz Elena , Höhle Barbara , Nazzi Thierry
Published: January 2025

Young infants can segment continuous speech with acoustic as well as statistical cues. Understanding how these cues interact can be informative about how infants solve the segmentation problem. This study investigates the use of acoustic and statistical cues by both adult French speakers and 6-to-7-month-old French-learning infants. Both groups were familiarized with a naturally recorded string, alternating either in duration (long-short) or in intensity (soft-loud). In addition, statistical cues were present in both strings, signaling different word boundaries than the acoustic cues. The adults were tested in a recognition task and the infants with the Head-turn Preference Procedure. Results show that the French-speaking adults segmented the strings by responding to the acoustic cues in both familiarization conditions, following the predictions of the Iambic-Trochaic Law. In contrast, the French-learning infants displayed segmentation based on TPs in the Intensity condition only. These findings collectively contribute to our understanding of how the use of acoustic and statistical cues to decode linguistic input changes between infancy and adulthood and differs across languages.
Towards the Origins of the Pyramid Texts
Authors: Peterková Hlouchová Marie , Krejčí Jaromír
Published: February 2025

The Pyramid Texts represent the oldest surviving corpus of religious compositions from ancient Egypt. Their first attestations come from the very end of the Fifth Dynasty, from the inner rooms of the pyramid of King Unas (late 24th century BC). Some scholars have suggested that their roots must have been older, and their origins have been widely discussed among Egyptologists. What could shed more light on this topic are the wooden fragments, dated to the Sixth Dynasty, with parts of several Pyramid Texts spells found in the excavations of the French Archaeological Mission at Saqqara. These artefacts demonstrate that the religious texts could have been written down on portable items of burial equipment, uncovering thus new dynamics in the extent of their usage. In this regard, wooden fragments from the pyramid complex Lepsius no. 24 (AC 22 [J]) and the pyramid complex of Queen Khentkaus II (AC 14 [A]) at Central Abusir, both dated to the Fifth Dynasty, are of considerable significance. These may represent the earliest clear evidence of the so-called Pyramid Texts in ancient Egypt.
Neo-Assyrian Imperial Religion Counts: a Quantitative Approach to the Affiliations of Kings and Queens with Their Gods and Goddesses
Authors: Gansell Amy , Alstola Tero , Jauhiainen Heidi , Svärd Saana
Published: February 2025

This article pursues a quantitative approach to measure and compare the frequency with which Neo-Assyrian kings and queens co-occur with deities across thousands of cuneiform documents. For the large corpus of kings’ texts, we used an automated method to collect data that is digitally available in the Open Richly Annotated Cuneiform Corpus (Oracc). For the much smaller corpus of queens’ texts, we collected data manually. As output, we offer new bodies of data on the textual co-occurrences of kings and queens with gods and goddesses. We interpret and rank these co-occurrence trends as royal-divine affiliations. Until now, these affiliations have only been qualitatively acknowledged and in some cases have been under-considered. In addition, most research on Neo-Assyrian imperial religion has focused on kings. Here we study both kings and queens (especially Naqiʾa) as we explore gendered religious practice and move toward an interpretation of the royal couple as a cooperative entity in Assyrian religion.
The Martyrdom of Vahan Goghtnatsi and its Spatio-Temporal Features
Authors: Shavarsh Azatyan
Published: February 2025

The Martyrdom of Vahan Goghtnatsi is a text that dates back to the beginning of the 8th century. Vahan was the son of Khosrov, a nobleman from Goghtn. In his early years, Vahan was taken hostage by the Arabs to Damascus. Subsequently, he returned to Armenia and converted to Christianity, a decision that resulted in his persecution. After visiting a number of locations, he proceeded to the court of Caliph Hisham (724–743) with the intention of requesting permission to practise his religion. His endeavours proved futile, and he was martyred in the city of Resafa in 736/737.

This article has concentrated on the forms of space and time that are directly related to early medieval thought. In addition to Christian paradigms, the martyrologist also employed mythical and traditional ideas. The contrast between the two sides of the world, the North and the South, was particularly evident. Concomitantly, these ideas are linked to the Armenian way of thinking about history, where the North is a metaphor for salvation and freedom. Moreover, the literal and metaphorical path occupies a significant place in the Martyrdom.

The pages of the Martyrdom are dominated by a concept of concrete time, whether in its linear or phased versions. In the first case, the occurrence of tangible, logical events is of paramount importance, whereas in the second, the passage of time, the progression of the day, and so forth, are of greater significance.