bluuebunny/crossref_metadata_2025 huggingface dataset:
Identification of galactofuranose antigens such as galactomannoproteins and fungal-type galactomannan from the yellow koji fungus (Aspergillus oryzae)
Authors: Kadooka Chihiro , Tanaka Yutaka , Hira Daisuke , Maruyama Jun-ichi , Goto Masatoshi , Oka Takuji
Published: February 2023

Filamentous fungi belonging to the genus Aspergillus are known to possess galactomannan in their cell walls. Galactomannan is highly antigenic to humans and has been reported to be involved in the pathogenicity of pathogenic filamentous fungi, such as A. fumigatus, and in immune responses. In this study, we aimed to confirm the presence of D-galactofuranose-containing glycans and to clarify the biosynthesis of D-galactofuranose-containing glycans in Aspergillus oryzae, a yellow koji fungus. We found that the galactofuranose antigen is also present in A. oryzae. Deletion of ugmA, which encodes UDP-galactopyranose mutase in A. oryzae, suppressed mycelial elongation, suggesting that D-galactofuranose-containing glycans play an important role in cell wall integrity in A. oryzae. Proton nuclear magnetic resonance spectrometry revealed that the galactofuranose-containing sugar chain was deficient and that core mannan backbone structures were present in ΔugmA A. oryzae, indicating the presence of fungal-type galactomannan in the cell wall fraction of A. oryzae. The findings of this study provide new insights into the cell wall structure of A. oryzae, which is essential for the production of fermented foods in Japan.
Ferroptosis: Underlying mechanism and the crosstalk with other modes of neuronal death after intracerebral hemorrhage
Authors: Cao Yuan , Xiao Wenbiao , Liu Shuzhen , Zeng Yi
Published: February 2023

Intracerebral hemorrhage (ICH) is a serious cerebrovascular disease with high rates of morbidity, mortality, and disability. Optimal treatment of ICH is a major clinical challenge, as the underlying mechanisms remain unclear. Ferroptosis, a newly identified form of non-apoptotic programmed cell death, is characterized by the iron-induced accumulation of lipid reactive oxygen species (ROS), leading to intracellular oxidative stress. Lipid ROS causes damage to nucleic acids, proteins, and cell membranes, eventually resulting in ferroptosis. In the past 10 years, ferroptosis has resulted in plenty of discoveries and breakthroughs in cancer, neurodegeneration, and other diseases. Some studies have also reported that ferroptosis does occur after ICH in vitro and in vivo and contribute to neuronal death. However, the studies on ferroptosis following ICH are still in the preliminary stage. In this review, we will summarize the current evidence on the mechanism underlying ferroptosis after ICH. And review the traditional modes of neuronal death to identify the crosstalk with ferroptosis in ICH, including apoptosis, necroptosis, and autophagy. Additionally, we also aim to explore the promising therapeutic application of ferroptosis in cell death-based ICH.
Case report: A novel homozygous histidine triad nucleotide-binding protein 1 mutation featuring distal hereditary motor-predominant neuropathy with rimmed vacuoles
Authors: Jiang Nan , Vazquez Do Campo Rocio , Kazamel Mohamed
Published: February 2023

Introduction

Recessive mutations in the gene encoding the histidine triad nucleotide-binding protein 1 (HINT1) are associated with axonal motor-predominant Charcot–Marie–Tooth (CMT) disease with neuromyotonia. A total of 24 HINT1 gene mutations have been reported so far. Some of these cases had mild to moderate elevations of creatinine kinase with no earlier reports of muscle biopsy findings in these cases. In this study, we describe a patient with axonal motor-predominant neuropathy and myopathy with rimmed vacuoles, likely due to a novel HINT1 gene mutation.

Case report

A 35-year-old African American man presented with insidious onset and progressive symmetric distal leg weakness followed by hand muscle atrophy and weakness since the age of 25. He had no muscle cramps or sensory complaints. His 38-year-old brother developed similar symptoms beginning in his early 30 s. On neurologic examination, the patient had distal weakness and atrophy in all limbs, claw hands, pes cavus, absent Achilles reflexes, and normal sensory examination. Electrodiagnostic studies revealed absent/reduced compound motor action potential amplitudes distally with normal sensory responses with no neuromyotonia. His sural nerve biopsy showed a chronic non-specific axonal neuropathy, and a biopsy of the tibialis anterior muscle demonstrated myopathic features and several muscle fibers harboring rimmed vacuoles without inflammation in addition to chronic denervation changes. A homozygous variant, p.I63N (c.188T > A), in the HINT1 gene was found in both brothers.

Conclusion

We describe a novel, likely pathogenic, HINT1 pI63N (c.188T > A) homozygous variant associated with hereditary axonal motor-predominant neuropathy without neuromyotonia in two African American brothers. The presence of rimmed vacuoles on muscle biopsy raises the possibility that mutations in the HINT1 gene may also cause myopathy.
Analysis of characteristics of intracranial cavernous angioma and bleeding factors in middle-aged and elderly patients
Authors: Xiu Chao , Ni Donghe , Zhao Jincui , Yu Yang
Published: February 2023

Objective

Intracranial cavernous angioma (ICA) is a cerebrovascular malformation. It causes local neurological dysfunction, epilepsy, intracranial hemorrhage (ICH) and other symptoms, seriously affecting the safety of patients. This study analyzed middle-aged and elderly patients with ICA in our hospital, summarized the characteristics of the disease and investigated the related factors of ICH.

Methods

We conducted a retrospective analysis of 120 middle-aged and elderly patients who were diagnosed with ICA by magnetic resonance imaging in our hospital from March 2018 to September 2021. The cases were assigned to either a bleeding group (i.e., the experimental group) or a non-bleeding group (i.e., the control group). The characteristics of the disease, including gender, age, number of lesions, form and symptoms of onset, distribution of lesions, blood supply vessels in the lesion area, size of the lesion and presence of bleeding, were summarized and analyzed. The relationship between these factors and ICH was investigated, and the data were analyzed using SPSS 25.0 software.

Results

There were 56 cases in the experimental group and 64 cases in the control group. A univariate analysis showed that gender, age, body mass index, blood lipids, number of lesions, course of the disease, onset of symptoms and disease characteristics were not associated with ICH in the middle-aged and elderly patients with ICA (P > 0.05). The maximum diameter, volume, location and blood supply area of the lesions were related to ICA complicated with ICH (P < 0.05). A multivariate unconditional logistic regression analysis revealed that the maximum diameter, volume, location and blood supply area of the lesions were independent risk factors for ICH in the middle-aged and elderly patients with ICA. The odds ratio (OR) of the maximum diameter of the lesion was 4.410, the OR of the lesion volume was 7.316, the OR of the lesion site was 7.470, and the OR of the blood supply area was 1.6588.

Conclusion

Intracranial cavernous angioma lesions in middle-aged and elderly patients occur mainly in the supratentorial area, with a small part located in the infratentorial area. The main form of the disease is chronic recurrence. The occurrence of bleeding is related to the size, location and blood supply of the lesion.
Effects of a high-prebiotic diet versus probiotic supplements versus synbiotics on adult mental health: The “Gut Feelings” randomised controlled trial
Authors: Freijy Tanya M. , Cribb Lachlan , Oliver Georgina , Metri Najwa-Joelle , Opie Rachelle S. , Jacka Felice N. , Hawrelak Jason A. , Rucklidge Julia J. , Ng Chee H. , Sarris Jerome
Published: February 2023

Background

Preliminary evidence supports the use of dietary interventions and gut microbiota-targeted interventions such as probiotic or prebiotic supplementation for improving mental health. We report on the first randomised controlled trial (RCT) to examine the effects of a high-prebiotic dietary intervention and probiotic supplements on mental health.

Methods

“Gut Feelings” was an 8-week, 2 × 2 factorial RCT of 119 adults with moderate psychological distress and low prebiotic food intake. Treatment arms: (1) probiotic supplement and diet-as-usual (probiotic group); (2) high-prebiotic diet and placebo supplement (prebiotic diet group); (3) probiotic supplement and high-prebiotic diet (synbiotic group); and (4) placebo supplement and diet-as-usual (placebo group). The primary outcome was assessment of total mood disturbance (TMD; Profile of Mood States Short Form) from baseline to 8 weeks. Secondary outcomes included anxiety, depression, stress, sleep, and wellbeing measures.

Results

A modified intention-to-treat analysis using linear mixed effects models revealed that the prebiotic diet reduced TMD relative to placebo at 8 weeks [Cohen’sd= −0.60, 95% confidence interval (CI) = −1.18, −0.03;p= 0.039]. There was no evidence of symptom improvement from the probiotic (d= −0.19, 95% CI = −0.75, 0.38;p= 0.51) or synbiotic treatments (d= −0.03, 95% CI = −0.59, 0.53;p= 0.92). Improved anxiety, stress, and sleep were noted in response to the prebiotic diet while the probiotic tentatively improved wellbeing, relative to placebo. No benefit was found in response to the synbiotic intervention. All treatments were well tolerated with few adverse events.

Conclusion

A high-prebiotic dietary intervention may improve mood, anxiety, stress, and sleep in adults with moderate psychological distress and low prebiotic intake. A synbiotic combination of high-prebiotic diet and probiotic supplement does not appear to have a beneficial effect on mental health outcomes, though further evidence is required. Results are limited by the relatively small sample size.

Clinical trial registration

https://www.anzctr.org.au/Trial/Registration/TrialReview.aspx?id=372753, identifier ACTRN12617000795392.
Topological network properties of resting-state functional connectivity patterns are associated with metal mixture exposure in adolescents
Authors: Invernizzi Azzurra , Rechtman Elza , Oluyemi Kristie , Renzetti Stefano , Curtin Paul , Colicino Elena , Ambrosi Claudia , Mascaro Lorella , Patrono Alessandra , Corbo Daniele , Cagna Giuseppa , Gasparotti Roberto , Reichenberg Abraham , Tang Cheuk Y. , Smith Donald R. , Placidi Donatella , Lucchini Roberto G. , Wright Robert O. , Horton Megan K.
Published: February 2023

Introduction

Adolescent exposure to neurotoxic metals adversely impacts cognitive, motor, and behavioral development. Few studies have addressed the underlying brain mechanisms of these metal–associated developmental outcomes. Furthermore, metal exposure occurs as a mixture, yet previous studies most often consider impacts of each metal individually. In this cross–sectional study, we investigated the relationship between exposure to neurotoxic metals and topological brain metrics in adolescents.

Methods

In 193 participants (53% females, ages: 15–25 years) enrolled in the Public Health Impact of Metals Exposure (PHIME) study, we measured concentrations of four metals (manganese, lead, copper, and chromium) in multiple biological media (blood, urine, hair, and saliva) and acquired resting–state functional magnetic resonance imaging scans. Using graph theory metrics, we computed global and local efficiency (global:GE; local:LE) in 111 brain areas (Harvard Oxford Atlas). We used weighted quantile sum (WQS) regression models to examine association between metal mixtures and each graph metric (GE or LE), adjusted for sex and age.

Results

We observed significant negative associations between the metal mixture and GE and LE [βGE = −0.076, 95% CI (−0.122, −0.031); βLE= −0.051, 95% CI (−0.095, −0.006)]. Lead and chromium measured in blood contributed most to this association for GE, while chromium measured in hair contributed the most for LE.

Discussion

Our results suggest that exposure to this metal mixture during adolescence reduces the efficiency of integrating information in brain networks at both local and global levels, informing potential neural mechanisms underlying the developmental toxicity of metals. Results further suggest these associations are due to combined joint effects to different metals, rather than to a single metal.
A bibliometric analysis of apoptosis in glaucoma
Authors: Zhang Jia-Heng , Wang Mei-Juan , Tan Ya-Ting , Luo Jia , Wang Shu-Chao
Published: February 2023

Background

Glaucoma is the first irreversible and second blindness disease, which is characterized by the death of retinal ganglion cells (RGCs) and degeneration of the optic nerve. Previous works have indicated that apoptosis is the main reason for RGC death in glaucoma. Although many studies have investigated the mechanism of apoptosis and different strategies targeting apoptosis to protect the RGCs and finally recover the impaired vision in the glaucoma. However, the global trend and hotspots of apoptosis in glaucoma have not been well illustrated and discussed.

Methods

Documents were extracted from the Web of Science Core Collection on November 2, 2022. We selected articles and reviews published in English from January 1, 1999 to November 1, 2022 to perform visual analysis and statistical analysis of countries, institutions, authors, references and keywords by VOSviewer 1.6.18 and CiteSpace 5.8.

Results

The publications about apoptosis in glaucoma show an increasing trend over time. Besides, the authors, institutions in the US and China published the most numbers of articles with the highest citation, which may be leading the research in the field of apoptosis in glaucoma. Last, series of advanced research results, technology and treatment for glaucoma, such as the discovery of key regulatory mechanisms on RGC apoptosis are emerging and will provide precise strategies for the treatment of glaucoma.

Conclusion

This research will broaden our comprehension about the role of apoptosis in the process of glaucoma, and provide guidelines for us in basic research and disease treatment in the further.
Pharmacokinetics of single- and multiple-dose flumatinib in patients with chronic phase chronic myeloid leukemia
Authors: Jiang Bo , Qi Junyuan , Sun Mingyuan , Zheng Weiwei , Wei Yongyue , Wang Jianxiang , Zhang Fengkui
Published: February 2023

Introduction

Flumatinib is a novel, oral breakpoint cluster region-abelson (BCR-ABL) tyrosine kinase inhibitor that has demonstrated manageable safety and promising efficacy in patients with newly diagnosed chronic phase (CP) chronic myeloid leukemia (CML).

Methods

This study evaluated the pharmacokinetic (PK) profiles of flumatinib mesylate tablets at a dose of 400 mg and 600 mg in patients with CML-CP. The study was registered at chictr.org Identifier (ChiCTR2100044700). In this open-label, pharmacokinetic study, eligible patients were administered a single-dose of flumatinib 400 mg or 600 mg on day 1, followed by 2-day washout and 8 consecutive days of once-daily administration. Serial plasma samples were assayed for flumatinib and its metabolites (N-demethylate metabolite M1 and amide-bond hydrolytic metabolite M3).

Results

Twenty-nine patients were assigned to flumatinib 400 mg (n=14) or 600 mg (n=15). Serum concentrations of flumatinib reached maximum measured plasma concentration (Cmax) at a median time of 2 hours after each single dose, and then eliminated slowly with a mean apparent terminal disposition half-life (t1/2) from 16.0 to 16.9 hours. Following single- and multiple-dose administration, flumatinib exposure (Cmax, area under the concentration-time curve from 0 to t hours (AUC0-t), area under the concentration-time curve from 0 hours to infinity (AUC0-∞)) increased in an approximately dose-proportional manner. There was approximately 4.1- and 3.4- fold drug accumulation at steady-state after multiple-dose administration at 400 mg and 600 mg, respectively. The drug-related AEs associated with both treatments were primarily low-grade and tolerable events.

Conclusion

Analysis of PK parameters indicated that flumatinib exposure increased in an approximately dose-proportional manner. Further research needs to be conducted in a large sample-size study.
Dynamic radiomics for predicting the efficacy of antiangiogenic therapy in colorectal liver metastases
Authors: Qu Hui , Zhai Huan , Zhang Shuairan , Chen Wenjuan , Zhong Hongshan , Cui Xiaoyu
Published: February 2023

Background and objective

For patients with advanced colorectal liver metastases (CRLMs) receiving first-line anti-angiogenic therapy, an accurate, rapid and noninvasive indicator is urgently needed to predict its efficacy. In previous studies, dynamic radiomics predicted more accurately than conventional radiomics. Therefore, it is necessary to establish a dynamic radiomics efficacy prediction model for antiangiogenic therapy to provide more accurate guidance for clinical diagnosis and treatment decisions.

Methods

In this study, we use dynamic radiomics feature extraction method that extracts static features using tomographic images of different sequences of the same patient and then quantifies them into new dynamic features for the prediction of treatmentefficacy. In this retrospective study, we collected 76 patients who were diagnosed with unresectable CRLM between June 2016 and June 2021 in the First Hospital of China Medical University. All patients received standard treatment regimen of bevacizumab combined with chemotherapy in the first-line treatment, and contrast-enhanced abdominal CT (CECT) scans were performed before treatment. Patients with multiple primary lesions as well as missing clinical or imaging information were excluded. Area Under Curve (AUC) and accuracy were used to evaluate model performance. Regions of interest (ROIs) were independently delineated by two radiologists to extract radiomics features. Three machine learning algorithms were used to construct two scores based on the best response and progression-free survival (PFS).

Results

For the task that predict the best response patients will achieve after treatment, by using ROC curve analysis, it can be seen that the relative change rate (RCR) feature performed best among all features and best in linear discriminantanalysis (AUC: 0.945 and accuracy: 0.855). In terms of predicting PFS, the Kaplan–Meier plots suggested that the score constructed using the RCR features could significantly distinguish patients with good response from those with poor response (Two-sided P<0.0001 for survival analysis).

Conclusions

This study demonstrates that the application of dynamic radiomics features can better predict the efficacy of CRLM patients receiving antiangiogenic therapy compared with conventional radiomics features. It allows patients to have a more accurate assessment of the effect of medical treatment before receiving treatment, and this assessment method is noninvasive, rapid, and less expensive. Dynamic radiomics model provides stronger guidance for the selection of treatment options and precision medicine.
Advances in application of swept-source optical coherence tomography angiography in diagnosis and treatment of diabetic retinopathy
Authors: Zhang Jinyan , Huo Qianqian , Xia Deyu , Wang Mingfang , Li Xiuyun
Published: February 2023

Diabetic retinopathy (DR) is the most common microvascular complication of diabetes and one of the leading causes of global blinding. More attention should be paid to the diagnosis, treatment and prognosis of DR. Swept-source optical coherence tomography angiography (SS-OCTA) is a novel imaging technique presented in recent years. It can accurately present the various levels of the retina, choriocapillaris, macula, and the optic papillary microcirculation, which is new to the diagnosis and prognosis of DR. However, SS-OCTA is limited by poor fixation or severe media clouding and is susceptible to motion artefacts and segmentation errors. Future limitations need to be addressed and large prospective trials conducted to refine the relevance of SS-OCTA to DR. The present study reviews the advances in clinical application of SS-OCTA in diagnosis, treatment and prognosis of DR.
Anti-AQP4–IgG-positive Leigh syndrome: A case report and review of the literature
Authors: Chen Jun , Wang Jianjun , Gan Jing , Luo Rong , Yang Zuozhen , Liang Mengmeng , Chen Xiaolu
Published: February 2023

Background

Leigh syndrome (LS; OMIM: 256000) is a progressive neurodegenerative disease caused by genetic mutations resulting in mitochondrial oxidative phosphorylation defects. The prognosis is poor, with most children dying before the age of 2 years. MT-ATP6 variants are the most common mitochondrial DNA mutations in LS. MT-ATP6 variant-induced LS may trigger autoimmunity, and immunotherapy might be effective. Here, we present the first pediatric case of anti-aquaporin 4 (AQP4)–IgG-positive LS caused by an MT-ATP6 variant.

Case

A 1-year-old boy was hospitalized due to recurrent fever, cough, and developmental regression. Two months previously, he had developed reduced responses to stimulation and psychomotor retardation. After admission, his condition deteriorated and respiratory failure ensued. Magnetic resonance imaging of the brain showed symmetrical small patchy abnormal signals around the third ventricle, pons, and dorsal periaqueductal gray matter in the dorsal medulla. Laboratory tests revealed anti-AQP4–IgG antibodies. Anti-infection, immunoglobulin, and glucocorticoid therapy were administered for symptomatic treatment. Genetic testing revealed a de novo homogeneous pathogenic variant of MT-ATP6 (m.9176T > C, mutation ratio: 99.97%). The patient was diagnosed with anti-AQP4–IgG-positive LS, treated with “cocktail therapy” (vitamins B1, B2, C, and E, l-carnitine, and coenzyme Q10), and discharged after his condition improved. A literature review revealed that LS-induced mitochondrial defects can impact the immune system; hence, immunotherapy and early mitochondrial cocktail therapy may improve outcomes.

Conclusion

Anti-AQP4–IgG-positive LS is very rare. Patients with LS with the m.9176T  > C variant of MT-ATP6 may be susceptible to autoimmune damage of the central nervous system. Early cocktail therapy combined with immunotherapy may improve their prognosis.
Cryptochrome 1 activation inhibits melanogenesis and melanosome transport through negative regulation of cAMP/PKA/CREB signaling pathway
Authors: Gao Rongyin , Zhang Ximei , Zou Kun , Meng Duo , Lv Jinpeng
Published: February 2023

Cutaneous pigmentation was recently shown to be an event regulated by clock proteins. Cryptochrome (CRY) is a key protein composing the feedback loop of circadian clock, however, the function of CRY in melanocytes remains unclear. Here, we found that KL001, a synthetic small molecule modulator of CRY1, inhibited melanin synthesis, as well as reduced melanocyte dendrite elongation and melanosome transport. In addition, the dominant role of CRY1 in KL001-induced anti-melanogenesis was revealed by small interfering RNA transfection. Cellular tyrosinase activity and expression level of melanogenic proteins, including tyrosinase, TRP-1, TRP-2, and transport proteins like Rab27a, Cdc42 and Myosin Va induced by α-MSH were remarkably reversed after KL001 treatment. Mechanistically, CRY1 activation inhibited melanogenesis through CREB-dependent downregulation of MITF and CREB phosphorylation was mediated by classical cAMP/PKA pathway. In addition, the other CRY1 activator, KL044 also suppressed cAMP/PKA/CREB pathway and inhibited melanogenesis. Finally, anti-melanogenic efficacy of KL001 was confirmed by determination of melanin contents in UVB-tanning model of brown guinea pigs, which indicated that targeting CRY1 activity, via topical application of small molecule activator, can be utilized therapeutically to manage human pigmentary disorders.
A systematic and comprehensive analysis of T cell exhaustion related to therapy in lung adenocarcinoma tumor microenvironment
Authors: Hu Peipei , Ma Jiahao , Chen Jinjian
Published: February 2023

Background: T cell exhaustion (TEX) is an important immune escape mechanism, and an in-depth understanding of it can help improve cancer immunotherapy. However, the prognostic role of TEX in malignant lung adenocarcinoma (LUAD) remains unclear.

Methods: Through TCGA and GEO datasets, we enrolled a total of 498 LUAD patients. The patients in TCGA-LUAD were unsupervised clustered into four clusters according to TEX signaling pathway. WGCNA analysis, survival random forest analysis and lasso regression analysis were used to select five differentially expressed genes among different clusters to construct a TEX risk model. The risk model was subsequently validated with GEO31210. By analyzing signaling pathways, immune cells and immune checkpoints using GSEA, GSVA and Cibersortx, the relationship between TEX risk score and these variables was evaluated. In addition, we further analyzed the expression of CCL20 at the level of single-cell RNA-seq and verified it in cell experiments.

Results: According to TEX signaling pathway, people with better prognosis can be distinguished. The risk model constructed by CD109, CCL20, DKK1, TNS4, and TRIM29 genes could further accurately identify the population with poor prognosis. Subsequently, it was found that dendritic cells, CD44 and risk score were closely related. The final single-cell sequencing suggested that CCL2O is a potential therapeutic target of TEX, and the interaction between TEX and CD8 + T is closely related.

Conclusion: The classification of T cell depletion plays a crucial role in the clinical decision-making of lung adenocarcinoma and needs to be further deepened.
Optical solitons in birefringent fibers with the generalized coupled space–time fractional non-linear Schrödinger equations
Authors: Fu Lei , Li Jingjing , Yang Hongwei , Dong Huanhe , Han Xiaofeng
Published: February 2023

The nonlinear Schrödinger (NLS) equation is an ideal model for describing optical soliton transmission. This paper first introduces an integer-order generalized coupled NLS equation describing optical solitons in birefringence fibers. Secondly, the semi-inverse and fractional variational method is used to extend the integer‐order model to the space–time fractional order. Moreover, various nonlinear forms of fractional NLS equations are discussed, including the Kerr, power, parabolic, dual-power, and log law. The exact soliton solutions, such as bright, dark, and singular solitons, are given. Finally, the behavior of the solution is shown by three-dimensional figures with different fractional orders, which reveals the propagation characteristics of optical solitons in birefringence fibers described by the generalized coupled space–time fractional NLS equation.
Cerebrovascular reactivity measurements using 3T BOLD MRI and a fixed inhaled CO2 gas challenge: Repeatability and impact of processing strategy
Authors: Sleight Emilie , Stringer Michael S. , Mitchell Isla , Murphy Madeleine , Marshall Ian , Wardlaw Joanna M. , Thrippleton Michael J.
Published: February 2023

Introduction: Cerebrovascular reactivity (CVR) measurements using blood oxygen level dependent (BOLD) magnetic resonance imaging (MRI) are commonly used to assess the health of cerebral blood vessels, including in patients with cerebrovascular diseases; however, evidence and consensus regarding reliability and optimal processing are lacking. We aimed to assess the repeatability, accuracy and precision of voxel- and region-based CVR measurements at 3 T using a fixed inhaled (FI) CO2 stimulus in a healthy cohort.

Methods: We simulated the effect of noise, delay constraints and voxel- versus region-based analysis on CVR parameters. Results were verified in 15 healthy volunteers (28.1±5.5 years, female: 53%) with a test-retest MRI experiment consisting of two CVR scans. CVR magnitude and delay in grey matter (GM) and white matter were computed for both analyses assuming a linear relationship between the BOLD signal and time-shifted end-tidal CO2 (EtCO2) profile.

Results: Test-retest repeatability was high [mean (95% CI) inter-scan difference: −0.01 (−0.03, −0.00) %/mmHg for GM CVR magnitude; −0.3 (−1.2,0.6) s for GM CVR delay], but we detected a small systematic reduction in CVR magnitude at scan 2 versus scan 1, accompanied by a greater EtCO2 change [±1.0 (0.4,1.5) mmHg] and lower heart rate [−5.5 (−8.6,−2.4] bpm]. CVR magnitude estimates were higher for voxel- versus region-based analysis [difference in GM: ±0.02 (0.01,0.03) %/mmHg]. Findings were supported by simulation results, predicting a positive bias for voxel-based CVR estimates dependent on temporal contrast-to-noise ratio and delay fitting constraints and an underestimation for region-based CVR estimates.

Discussion: BOLD CVR measurements using FI stimulus have good within-day repeatability in healthy volunteers. However, measurements may be influenced by physiological effects and the analysis protocol. Voxel-based analyses should be undertaken with care due to potential for systematic bias; region-based analyses are more reliable in such cases.
Catecholamine concentrations in duck eggs are impacted by hen exposure to heat stress
Authors: Lyte Joshua M. , Lyte Mark , Daniels Karrie M. , Oluwagbenga Esther M. , Fraley Gregory S.
Published: February 2023

Rapid “fight-or-flight” responses to stress are largely orchestrated by the catecholamines. Moreover, catecholamines and catecholamine precursors are widely recognized to act as interkingdom signaling molecules among host and microbiota, as well as to serve as chemotactic signals for bacterial foodborne pathogens. While albumen and yolk concentrations of glucocorticoids have received extensive attention as non-invasive indicators of hen response to stress, little is known regarding the impact of the hen’s stress response on in ovo catecholamine and catecholamine precursor concentrations. The aim of the present study was to determine norepinephrine and L-dopa concentrations in albumen and yolk of eggs laid by hens maintained under normal or heat stress conditions. Norepinephrine and L-dopa concentrations were also measured in oviductal tissue. Breeder ducks (∼35 weeks/age) were kept under normal (22°C) conditions or subjected to cyclical heat stress (35°C day/29.5°C night) for 3 weeks. Eggs (n = 12 per timepoint/group) were collected on a weekly basis. Hens were sacrificed at baseline or after 3 weeks of heat stress for oviductal tissue collection. Albumen, yolk, and oviduct concentrations of norepinephrine and L-dopa were determined using ultra high-performance liquid chromatography with electrochemical detection. Norepinephrine and L-dopa were detected in oviductal tissue as well as egg albumen and yolk. Norepinephrine concentrations were elevated (p < 0.05) in the yolk of eggs laid by the heat stress group compared to those of the control group. Norepinephrine concentrations in albumen were elevated (p < 0.05) in the heat stress group compared to control group at week 2. L-dopa concentrations were not significantly affected (p > 0.05) by heat stress in albumen, yolk, or oviductal tissue. Together, the present study provides the first evidence of the stress neurohormone, norepinephrine, in duck eggs and identifies that hen exposure to heat stress can affect in ovo norepinephrine concentrations. These data highlight the potential utility of in ovo catecholamine concentrations as non-invasive measures of the hen’s response to stress, as well as warrants future research into whether hen deposition of stress-related neurochemicals into the egg could serve as a chemotactic signal in the vertical transmission of foodborne pathogens.
Drought stress tolerance mechanisms and their potential common indicators to salinity, insights from the wild watermelon (Citrullus lanatus): A review
Authors: Malambane Goitseone , Madumane Kelebogile , Sewelo Lesego T. , Batlang Utlwang
Published: February 2023

Climate change has escalated the effect of drought on crop production as it has negatively altered the environmental condition. Wild watermelon grows abundantly in the Kgalagadi desert even though the environment is characterized by minimal rainfall, high temperatures and intense sunshine during growing season. This area is also characterized by sandy soils with low water holding capacity, thus bringing about drought stress. Drought stress affects crop productivity through its effects on development and physiological functions as dictated by molecular responses. Not only one or two physiological process or genes are responsible for drought tolerance, but a combination of various factors do work together to aid crop tolerance mechanism. Various studies have shown that wild watermelon possess superior qualities that aid its survival in unfavorable conditions. These mechanisms include resilient root growth, timely stomatal closure, chlorophyll fluorescence quenching under water deficit as key physiological responses. At biochemical and molecular level, the crop responds through citrulline accumulation and expression of genes associated with drought tolerance in this species and other plants. Previous salinity stress studies involving other plants have identified citrulline accumulation and expression of some of these genes (chloroplast APX, Type-2 metallothionein), to be associated with tolerance. Emerging evidence indicates that the upstream of functional genes are the transcription factor that regulates drought and salinity stress responses as well as adaptation. In this review we discuss the drought tolerance mechanisms in watermelons and some of its common indicators to salinity at physiological, biochemical and molecular level.
The transcriptional regulatory network of hormones and genes under salt stress in tomato plants (Solanum lycopersicum L.)
Authors: Wang Baike , Wang Juan , Yang Tao , Wang Jinxin , Dai Qi , Zhang Fulin , Xi Rui , Yu Qinghui , Li Ning
Published: February 2023

Salt stress has become one of the main limiting factors affecting the normal growth and development of tomatoes as well as fruit quality and yields. To further reveal the regulatory relationships between tomato hormones under salt stress, the interaction between hormones and TF and the genome-wide gene interaction network were analyzed and constructed. After salt treatment, the levels of ABA, SA, and JA were significantly increased, the levels of GA were decreased, and IAA and tZ showed a trend of first increasing and then decreasing. The expression patterns of hormone biosynthesis and signal transduction related genes were analyzed based on RNA-seq analysis, the co-expression network of hormones and genome-wide co-expression networks were constructed using weighted gene co-expression network analysis (WGCNA). The expression patterns of specific transcription factors under salt stress were also systematically analyzed and identified 20 hormone-related candidate genes associated with salt stress. In conclusion, we first revealed the relationship between hormones and genes in tomatoes under salt stress based on hormone and transcriptome expression profiles and constructed a gene regulatory network. A transcriptional regulation model of tomato consisted of six types of hormones was also proposed. Our study provided valuable insights into the molecular mechanisms regulating salt tolerance in tomatoes.
Preschoolers’ anthropomorphizing of robots: Do human-like properties matter?
Authors: Goldman Elizabeth J. , Baumann Anna-Elisabeth , Poulin-Dubois Diane
Published: February 2023

Prior work has yielded contradicting evidence regarding the age at which children consistently and correctly categorize things as living or non-living. The present study tested children’s animacy judgments about robots with a Naïve Biology task. In the Naïve Biology task, 3- and 5-year-olds were asked if robots, animals, or artifacts possessed mechanical or biological internal parts. To gauge how much children anthropomorphize robots in comparison to animals and artifacts, children also responded to a set of interview questions. To examine the role of morphology, two robots were used: a humanoid robot (Nao) and a non-humanoid robot (Dash). To investigate the role of dynamic characteristics, children saw one robot behave in a goal-directed manner (i.e., moving towards a ball) and one robot exhibit non-goal-directed behavior (i.e., moving away from a ball). Children of both age groups correctly attributed biological insides to the animal and mechanical insides to the artifact. However, 3-year-olds seemed confused about what belonged inside both robots and assigned biological and mechanical insides equally. In contrast, 5-year-olds correctly assigned mechanical insides to both robots, regardless of the robot’s morphology or goal-directedness. Regarding the Animacy Interview, 3-year-olds performed at chance level when asked about the animacy of robots, animals, and artifacts. In contrast, 5-year-olds correctly attributed animacy to animals and accurately refrained from anthropomorphizing artifacts and the non-humanoid robot Dash. However, 5-year-olds performed at chance for Nao, suggesting they may be confused about the psychological properties of a human-looking robot. Taken together, these findings reveal a developmental transition during the preschool years in the attribution of biological and psychological properties to social robot.
Patriotism, nationalism, and evaluations of the government’s handling of the coronavirus crisis
Authors: Zhai Yida , Lu Yizhen , Wu Qidi
Published: February 2023

Introduction

The COVID-19 pandemic has been accompanied by a global rise of nationalism, and many countries’ responses to the pandemic have further intensified nationalist sentiments. The public is polarized over government policies toward the pandemic. Hence, this study examined the associations of patriotism and nationalism with the support for lockdown policies and evaluations of governmental performance.

Methods

We recruited 180 participants from one Chinese university.

Results

Patriotism and nationalism had a direct effect on evaluations of governmental performance. Patriotism also had indirect effects on favorable evaluations of governmental performance through the support of lockdown policies. In addition, this study examined the relationship of threat perception and evaluations of governmental performance in the pandemic.

Discussion

This relationship was found to be insignificant; however, the interaction effects between threat perception and patriotism on evaluations of governmental performance were significant. The implications of the study results are discussed.
Grandchild care and life satisfaction of older adults: Empirical evidence from China
Authors: Dong Xiangyu , Ling Hongxiang , Yang Tianyi , Wang Kun
Published: February 2023

Background

In China, grandchild care plays an important social role later in life. The effects of grandchild care on physical health and depression in older adults have been illustrated. However, there is a gap in research on grandchild care and life satisfaction of older adults specifically based on the Chinese experience.

Method

Based on 7,079 individuals’ data from 2018 China Longitudinal Aging Social Survey (CLASS), this study explored the impact of grandchild care on older adults’ life satisfaction by using Ordinary Least Squares (OLS), Propensity Score Matching (PSM), and instrumental variables (IV) models.

Results

The empirical results indicated that (1) life satisfaction was significantly higher for older adults who undertook grandchild care compared to those who did not; (2) non-coresiding grandparents showed higher life satisfaction than those non-carers, and this effect was not found in custodial grandparents or three-generation household grandparents; (3) higher life satisfaction of grandchild caregivers was achieved through reduced loneliness, enhanced self-efficacy, and increased emotional support from children, with the latter being the greatest contribution; and (4) the improving effect of grandchild care on life satisfaction was found mainly in the group of older adults who were male and in rural households.

Conclusion

There was a significant difference in life satisfaction between older Chinese adults who provided grandchild care and those who did not. Efforts in terms of old age policy protection and family relationships should be made to enhance the subjective well-being of older adults.
Parenting style and Chinese preschool children’s pre-academic skills: A moderated mediation model of approaches to learning and family socioeconomic status
Authors: Xia Xiaoying
Published: February 2023

This study examined the mediating role of children’s approaches to learning (ATL) in parenting style and Chinese preschoolers’ pre-academic skills (i.e., literacy and numeracy) as well as the moderating role of family socioeconomic status (SES) in the mediating process. Participants were 307 children aged five to six years old from four public kindergartens in Shanghai, China. Parents provided demographic information and reported their parenting style (i.e., authoritative, authoritarian, and permissive) and teachers rated each child’s ATL and pre-academic skills. Results indicated that: (1) authoritative parenting positively related to children’s pre-academic skills while no significant relationships were identified either for authoritarian or permissive parenting with pre-academic skills; (2) children’s ATL partially mediated the relationship between authoritative parenting and children’s pre-academic skills; (3) family SES moderated the relationship between children’s ATL and pre-academic skills. Specifically, children’s ATL was more strongly related to pre-academic skills for children from low SES families as compared to their high-SES peers. These findings contribute to the understanding of the effects of parenting styles on Chinese children’s early academic achievement and underscore the importance of ATL to children’s pre-academic skills, especially for low-SES children.
Dreaming during the COVID-19 pandemic: Support for the threat simulation function of dreams
Authors: Abbas Noor H. , Samson David R.
Published: February 2023

Evolutionary theories suggest that dreams function as a world simulator of events that maximizes our ability to surmount social and threat-related challenges critical to survivorship and reproduction. Here, in contrast to the incorporation continuity hypothesis, we test the (1) social bias hypothesis, which states that dreams will overrepresent positive social interactions relative to waking life, (2) the mutually exclusive threat bias hypothesis, the idea that dream content will be negative relative to waking life, (3) the strengthening hypothesis, which states that dreams will rehearse more positive interactions with individuals the self is familiar with relative to waking life, and (4) the compensation hypothesis, which states that social contents in dreams increases during periods of social seclusion. Dream (n = 168) and wake (n = 184) reports were collected through a standardized online survey from 24 undergraduate students. Recalls were analyzed using the Social Content Scale. Generalized linear mixed effects models were used, and the following fixed-effects were considered for the study; the number of reports contributed, report state, biological sex, stress, social support, and media exposures. Results showed support for the threat bias hypothesis, we found that dreams were more negative and featured more unfamiliar individuals in contrast to waking life. Additionally, we found partial support for the social bias and the strengthening hypotheses, however no support was shown for the compensation hypothesis. Overall, these results demonstrate support for the threat simulation function of dreams.
Everything, everywhere, all at once: A study of polychronicity, work-school facilitation, and emotional exhaustion in working students
Authors: Grogan Allan , Lilly Juliana
Published: February 2023

This study investigates how a preference for multitasking serves as a resource toward mitigating emotional exhaustion through the mediating role of work-school facilitation. Utilizing conservation of resources (COR) theory, we utilized a sample of 153 working students in a time-lagged study to examine the relationship between these variables. Results suggest that polychronicity serves as a valuable resource toward balancing the simultaneous needs of work and school, and ultimately, decreasing academic emotional exhaustion. The findings presented in this study further advance scholarship in this domain by highlighting our understanding of polychronicity as an individual difference variable in facilitating role balance within the school–work domain. Practical implications and limitations are discussed.
Face-specific negative bias of aesthetic perception in depression: Behavioral and EEG evidence
Authors: Chen Zhitang , Wang Zhenghua , Shen Yuhua , Zeng Suhua , Yang Xiangyu , Kuang Yifang , Dou Zheng , Wang Lihui , Li Weidong
Published: February 2023

Introduction

Symptoms of depression are associated with the dysfunction of neural systems such as the emotion, reward system, and the default mode network. These systems were suggested by the model of neuroaesthetics as key contributions to aesthetic experience, leading to the prediction of atypical aesthetic orientation in depression. Here we investigated the aesthetic characteristics of depression and the corresponding neural underpinnings.

Methods

Fifty-two (25 depression patients, 27 healthy controls) participants were asked to make aesthetic judgments on faces and landscapes in an electroencephalographic (EEG) experiment.

Results

Our results indicate that relative to the controls, the depression tended to give ugly judgments and refrained from giving beautiful judgments, which was observed only for faces but not for landscapes. We also found that the face-induced component N170 was more negative in the depression group than the control group for ugly and neutral faces. Moreover, the aesthetic evaluation of ugly faces was associated with decreased N200 negativity in the depression group than in the control group, while the evaluation of beautiful faces was associated with decreased brain synchronization at the theta band.

Discussion

These results suggested a face-specific negative aesthetic bias in depression which can help to design and develop aesthetics-oriented schemes in assisting the clinical diagnosis and therapy of depression.