bluuebunny/crossref_metadata_2025 huggingface dataset:
3Es for AI: Economics, Explanation, Epistemology
Authors: Kaul Nitasha
Published: March 2022

This article locates its roots/routes in multiple disciplinary formations and it seeks to advance critical thinking about an aspect of our contemporary socio-technical challenges by bracketing three knowledge formations—artificial intelligence (AI), economics, and epistemology—that have not often been considered together. In doing so, it responds to the growing calls for the necessity of further transdisciplinary engagements that have emanated from work in AI and also from other disciplines. The structure of the argument here is as follows. First, I begin by demonstrating how and why explanation is a problem in AI (“XAI problem”) and what directions are being taken by recent research that draws upon social sciences to address this, noting how there is a conspicuous lack of reference in this literature to economics. Second, I identify and analyze a problem of explanation that has long plagued economics too as a discipline. I show how only a few economists have ever attempted to grapple with this problem and provide their perspectives. Third, I provide an original genealogy of explanation in economics, demonstrating the changing nature of what was meant by an explanation. These systematic changes in consensual understanding of what occurs when something is said to have been “explained”, have reflected the methodological compromises that were rendered necessary to serve different epistemological tensions over time. Lastly, I identify the various relevant historical and conceptual overlaps between economics and AI. I conclude by suggesting that we must pay greater attention to the epistemologies underpinning socio-technical knowledges about the human. The problem of explanation in AI, like the problem of explanation in economics, is perhaps not only, or really, a problem of satisfactory explanation provision alone, but interwoven with questions of competing epistemological and ethical choices and related to the ways in which we choose sociotechnical arrangements and offer consent to be governed by them.
Effects of Occasional and Habitual Wearing of High-Heeled Shoes on Static Balance in Young Women
Authors: Yamada-Yanagawa Ayano , Sasagawa Shun , Nakazawa Kimitaka , Ishii Naokata
Published: March 2022

The purpose of this study was to examine the effects of occasional and habitual wearing of high-heeled shoes on static balance in young women. Groups of habitual high-heel wearers and non-wearers (n = 7 in both groups) were asked to stand quietly on a force platform without shoes (WS condition) or with high heels (heel area 1 cm2, heel height 7 cm) (HH condition). During the trials, the center-of-pressure (CoP) position in the anterior-posterior direction was measured, and its root mean square (as a measure of postural sway magnitude, CoPRMS) and mean velocity (as a measure of regulatory activity, CoPMV) were calculated. To further examine the effect of high-heel wearing on the temporal aspects of slow and fast processes in static balance, the CoP sway was decomposed into low- (below 0.5 Hz) and high- (above 0.5 Hz) frequency components, and then spectral analysis was performed. Results showed that the CoPRMS was not significantly different between the groups or between the shoe conditions, indicating that wearing high heels with a heel height of 7 cm did not increase the magnitude of postural sway, irrespective of high-heel experience. The CoPMV was significantly larger in the HH condition than in the WS condition, whereas it was not significantly different between the groups. This result indicates that wearing high heels increased the amount of regulatory activity in both habitual wearers and non-wearers. The spectral analysis further showed that habitual high-heel wearers showed significantly decreased rate of regulatory activity than non-wearers, both while standing with and without high heels. These results suggest that use-dependent changes in static balance control are evident in both high-heeled and without shoes conditions.
A Coronal Landmark for Tibial Component Positioning With Anatomical Alignment in Total Knee Arthroplasty: A Radiological and Clinical Study
Authors: Gong Tianlun , Wang Ruoyu , Gong Song , Han Lizhi , Yi Yihu , Wang Yuxiang , Xu Weihua
Published: March 2022

Objective

The purpose of this study was to investigate the value of the lateral point of articular surface of distal tibia (LADT) for anatomical alignment in total knee arthroplasty.

Methods

We reconstructed 148 three-dimensional pre-arthritic tibias and measured the tibial component inclination angle corresponding to the distal landmark of LADT. A retrospective study included 81 TKA recipients divided into the AA group and MA group. Clinical assessments including ROM, HSS, WOMAC, satisfaction for surgery, and radiological assessment were evaluated at one-year follow-up.

Results

The tibial component varus angle corresponding to the distal landmark of LADT in the male and female groups were 3.4 ± 0.3° (2.6~4.2°) and 3.2 ± 0.3° (2.3~4.0°), respectively (P <0.05). Using LADT as the distal landmark for extramedullary tibial cutting guidance, the medial proximal tibia angle (MPTA) of the AA group was 87.0±1.2° (85.0~90.0°), and the AA and MA technique showed no difference in improvement in postoperative knee functional recovery at final follow-up.

Conclusions

This study preliminarily indicated that LADT can be a reliable and economical landmark for coronal plane alignment of the tibial component.
Assessment of Antimicrobial Effects on Broiler Gut Barrier Through Histopathology and Immunohistochemistry of Tight-Junction Proteins
Authors: Cuccato Matteo , Scaglione Frine Eleonora , Centelleghe Cinzia , Divari Sara , Biolatti Bartolomeo , Pregel Paola , Cannizzo Francesca Tiziana
Published: March 2022

In recent years, antimicrobial (AM) use in poultry farming has been attracting attention worldwide mainly due to AM resistance spreading. The role of AM prophylaxis in the modulation of gut microbiota, as well as of gut health, is still not clearly understood. Therefore, this study aimed to investigate the role of different prophylaxis protocols in the modulation of the gut barrier in broilers by applying a histopathological approach. Intestinal tissue samples were collected from a total of 240 male broilers (Ross 306), reared and treated with different AM protocols. Haematoxylin and Eosin (HE) staining and a multiple scoring system were used to evaluate the presence of lesions in ileum, cecum and colon of treated broilers. Moreover, immunohistochemistry (IHC) was performed to assess the expression of claudin-3 and ZO-1 proteins in intestinal tissues. The application of a semi-quantitative scoring system was used in IHC stained samples. HE results revealed that intestinal tissues were mainly characterized by epithelial detachment and fusion of the intestinal villi, but also by the presence of lymphocytic infiltrate in the mucosa and submucosa of AM-treated broilers. However, the IHC approach for the evaluation of claudin-3 and ZO-1 proteins showed that their expression was not affected by the different AM treatments. Nevertheless, the presence of intestinal lesions highlighted by histopathology suggests that AM treatments could harm the gut health of broilers, inducing an inflammatory response and consequent epithelial lesions. In order to clarify the role of AM treatments in the modulation of gut barrier in broilers, further studies are needed.
Pattern of Antibiotic Consumption in Two Italian Production Chains Differing by the Endemic Status for Porcine Reproductive and Respiratory Syndrome
Authors: Trevisi Paolo , Amatucci Laura , Ruggeri Roberta , Romanelli Costanza , Sandri Giampietro , Luise Diana , Canali Massimo , Bosi Paolo
Published: March 2022

The aim of this case study was to quantify antibiotic (AB) use in Italian weaning (W) and fattening (F) units differentiated for porcine reproductive and respiratory syndrome (PRRS) occurrence. Farms were classified as either PRRS negative (–) or PRRS positive (+) based on the circulation of the virus among the animals. In all the farms, the modified live PRRS virus (PRRSV) vaccine was provided to all the animals. In the PRRS– farms, the level of circulating antibodies was low, and the disease, in its clinical form, did not occur. In the PRRS+ farms, the level of circulating antibodies against the virus was high, and the disease was recurrent. Data regarding AB consumption were collected from 2017 to 2020, and the active compounds (ACs) were expressed as milligrams of AC/total kilogram of body weight (BW) produced. Each AC was classified into one of four categories according to the European Medicines Agency classification of ABs for prudent and responsible use in animals: Avoid, Restrict, Caution, and Prudence. Data regarding the ACs in each category were analyzed using a linear model that included production phase, PRRS status, and their interaction as factors. Performance parameters, average age of the pigs at the end of each phase, daily live weight gain, feed-to-gain ratio, total losses, cost index, and medication costs were significantly influenced by the PRRS chain. The use of class B ABs was not affected by production phase or PRRS status. Conversely, for class C ABs, interaction between the two factors (p= 0.02) was observed; W/PRRS+ and F/PRRS+ showed the greatest AB use for this class (p= 0.003). For class D ABs, the interaction was significant (p= 0.01); class C and D ABs were used more in the weaning (p= 0.07) than in the fattening phase (p= 0.003). For the weaning phase, the use of class C and D ABs was greater in the PRRS+ than in the PRRS– chain (p< 0.01). In conclusion, PRRS status affected the growth of pigs and economic performance. Moreover, PRRS status significantly influenced the use of ABs during all the growing periods with the greatest impact being on the weaning phase.
Spatiotemporal Analysis and Assessment of Risk Factors in Transmission of African Swine Fever Along the Major Pig Value Chain in Lao Cai Province, Vietnam
Authors: Lee Hu Suk , Dao Tung Duy , Huyen Le Thi Thanh , Bui Vuong Nghia , Bui Anh Ngoc , Ngo Dung Tien , Pham Uyen Ba
Published: March 2022

African swine fever (ASF) is a contagious and lethal hemorrhagic disease with a case fatality rate approaching 100% in domestic pigs. The main objectives of this study were to describe the spatiotemporal analysis as well as to assess the potential risk factors along the pig value chain in Lao Cai province, Vietnam. A total of 925 outbreaks were reported from 2019 to 2020. The three clusters (primary, secondary and 5th) were observed near the Chinese border. The most temporal clusters were detected between May and August during the study period. In addition, we evaluated the association between ASF outbreak locations to the nearest main roads and elevation. For ASF outbreak locations to the nearest main roads, compared with the reference (<5,000 m), <1,000 m (10.22 times) and 1,000–2,000 m (1.98 times) were significantly higher occurrences of ASF. For elevation, compared to the reference (>1,500 m), the farm locations with <500 m (55.31 times) showed a significantly increased risk of ASF outbreaks. Farmers perceived that the highest risk of ASF transmission may come from collectors and slaughterers, intermediaries inside and outside the commune, feed agents and maize agents in the commune, and pig retailers. Both commercial and household pig producers considered minimizing the number of people going in and out of pig stables and improving healthcare and husbandry procedures to be both very important and feasible. There is a need for compliance by all pig producers and other actors in the pig value chain to adopt biosecurity practices. Therefore, awareness, knowledge and understanding of infection and risks of ASF need to be improved. Veterinary officials at the provincial and district levels need to improve capacity and resources to perform laboratory analysis for ASF and need to coordinate with local actors on the control and prevention of ASF in the community.
Minimum Alveolar Concentration of Isoflurane in Rats Chronically Treated with the Synthetic Cannabinoid WIN 55,212-2
Authors: Chavez-Monteagudo Julio Raul , Ibancovichi José Antonio , Sanchez-Aparicio Pedro , Recillas-Morales Sergio , Osorio-Avalos Jorge , De Paz-Campos Marco Antonio
Published: March 2022

The minimum alveolar concentration MAC of isoflurane was measured in rats chronically treated with WIN 55,212-2. Methods: The MAC of isoflurane was determined in 24 male rats from expiratory samples at time of tail clamping under the following conditions: without treatment MAC(ISO), in rats treated for 21 days with WIN 55,212-2 MAC(ISO + WIN55), and in rats 8 days after stopping treatment with WIN 55,212-2 (MACISO + WIN55 + 8D). Results: The MAC(ISO) was 1.32 ± 0.06. In the MAC(ISO + WIN55) group, the MAC increased to 1.69 ± 0.09 (28%, p-value ≤ (0.0001). Eight days after stopping treatment with WIN55, the MAC did not decrease significantly, 1.67 ± 0.07 (26%, p-value ≤ 0.0001). Conclusions: The administration of WIN 55,212-2 for 21 days increases the MAC of isoflurane in rats. This effect does not disappear 8 days after discontinuation of treatment with the synthetic cannabinoid.
Pharmacokinetic/Pharmacodynamic Analysis and Dose Optimization of Cefmetazole and Flomoxef against Extended-Spectrum β-Lactamase-Producing Enterobacterales in Patients with Invasive Urinary Tract Infection Considering Renal Function
Authors: Hamada Yukihiro , Kasai Hidefumi , Suzuki-Ito Moeko , Matsumura Yasufumi , Doi Yohei , Hayakawa Kayoko
Published: March 2022

The optimal regimens of cefmetazole and flomoxef for the treatment of urinary tract infections caused by extended-spectrum β-lactamase (ESBL)-producing Enterobacterales are not well defined. Our study found that the pharmacokinetic/pharmacodynamic targets for cefmetazole and flomoxef were 70% T > MIC, which is suggestive of bactericidal activity. A Monte Carlo simulation (MCS) was performed using the published data to calculate a new probability of target attainment (PTA ≥ 90%) for each renal function. The MCS was performed with 1000 replicates, and clinical breakpoints were calculated to attain PTA ≥ 90% for creatinine clearance (CCR) of 10, 30, 50, and 70 mL/min. The 90% ≥ PTA (70% T > MIC) of cefmetazole and flomoxef in patients who received a standard regimen (0.5 or 1 g, 1 h injection) for each renal function was calculated. Our results suggest that in patients with CCR of less than 30, 31–59, and more than 60 mL/min, the optimal dosage of cefmetazole would be 1 g q12 h, 1 g q8 h, and 1 g q6 h, respectively. Furthermore, in patients with CCR of less than 10, 10–50, and more than 50 mL/min, the optimal dosage of flomoxef would be 1 g q24 h, 1 g q8 h or 12 h, and 1 g q6 h, respectively.
Effects of Antioxidant Intake on Fetal Development and Maternal/Neonatal Health during Pregnancy
Authors: Sebastiani Giorgia , Navarro-Tapia Elisabet , Almeida-Toledano Laura , Serra-Delgado Mariona , Paltrinieri Anna Lucia , García-Algar Óscar , Andreu-Fernández Vicente
Published: March 2022

During pregnancy, cycles of hypoxia and oxidative stress play a key role in the proper development of the fetus. Hypoxia during the first weeks is crucial for placental development, while the increase in oxygen due to the influx of maternal blood stimulates endothelial growth and angiogenesis. However, an imbalance in the number of oxidative molecules due to endogenous or exogenous factors can overwhelm defense systems and lead to excessive production of reactive oxygen species (ROS). Many pregnancy complications, generated by systemic inflammation and placental vasoconstriction, such as preeclampsia (PE), fetal growth restriction (FGR) and preterm birth (PTB), are related to this increase of ROS. Antioxidants may be a promising tool in this population. However, clinical evidence on their use, especially those of natural origin, is scarce and controversial. Following PRISMA methodology, the current review addresses the use of natural antioxidants, such as epigallocatechin gallate (EGCG), melatonin and resveratrol (RESV), as well as other classical antioxidants (vitamin C and E) during the prenatal period as treatment of the above-mentioned complications. We review the effect of antioxidant supplementation on breast milk in lactating mothers.
Metabolic Stress and Mitochondrial Dysfunction in Ataxia-Telangiectasia
Authors: Subramanian Goutham Narayanan , Yeo Abrey Jie , Gatei Magtouf Hnaidi , Coman David John , Lavin Martin Francis
Published: March 2022

The ataxia-telangiectasia mutated (ATM) protein kinase is, as the name implies, mutated in the human genetic disorder ataxia-telangiectasia (A-T). This protein has its “finger in many pies”, being responsible for the phosphorylation of many thousands of proteins in different signaling pathways in its role in protecting the cell against a variety of different forms of stress that threaten to perturb cellular homeostasis. The classical role of ATM is the protection against DNA damage, but it is evident that it also plays a key role in maintaining cell homeostasis in the face of oxidative and other forms of non-DNA damaging stress. The presence of ATM is not only in the nucleus to cope with damage to DNA, but also in association with other organelles in the cytoplasm, which suggests a greater protective role. This review attempts to address this greater role of ATM in protecting the cell against both external and endogenous damage.
Role of Selenium and Vitamins E and B9 in the Alleviation of Bovine Mastitis during the Periparturient Period
Authors: Khan Muhammad Zahoor , Ma Yulin , Xiao Jianxin , Chen Tianyu , Ma Jiaying , Liu Shuai , Wang Yajing , Khan Adnan , Alugongo Gibson Maswayi , Cao Zhijun
Published: March 2022

Mastitis (inflammation of the mammary gland) commonly occurs in dairy cattle during the periparturient period (transition period), in which dairy cattle experience physiological and hormonal changes and severe negative energy balance, followed by oxidative stress. To maintain successful lactation and combat negative energy balance (NEB), excessive fat mobilization occurs, leading to overproduction of reactive oxygen species (ROS). Excessive fat mobilization also increases the concentrations of nonesterified fatty acids (NEFA) and β-hydroxybutyric acid (BHB) during the periparturient period. In addition, the excessive utilization of oxygen by cellular respiration in the mammary causes abnormal production of oxidative stress (OS). OS impairs the immunity and anti-inflammatory efficiency of periparturient dairy cattle, increasing their susceptibility to mastitis. To alleviate oxidative stress and subsequent mastitis, antioxidants are supplemented to dairy cattle from an external source. Extensive studies have been conducted on the supplementation of selenium (Se) and vitamins E and B9 to mitigate mastitis during the transition period in dairy cattle. Altogether, in the current review, we discuss the research development on bovine mastitis and its major causes, with special emphasis on oxidative stress during the transition period. Moreover, we discuss the antioxidant, immunoregulatory, and anti-inflammatory properties of Se and vitamins E and B9 and their role in the control of bovine mastitis in periparturient dairy cattle.
A Pathway-Based Genetic Score for Oxidative Stress: An Indicator of Host Vulnerability to Phthalate-Associated Adverse Neurodevelopment
Authors: Tanner Samuel , Thomson Sarah , Drummond Katherine , O’Hely Martin , Symeonides Christos , Mansell Toby , Saffery Richard , Sly Peter D. , Collier Fiona , Burgner David , Sugeng Eva J. , Dwyer Terence , Vuillermin Peter , Ponsonby Anne-Louise ,   on behalf of the Barwon Infant Study Investigator Group
Published: March 2022

The developing brain is highly sensitive to environmental disturbances, and adverse exposures can act through oxidative stress. Given that oxidative stress susceptibility is determined partly by genetics, multiple studies have employed genetic scores to explore the role of oxidative stress in human disease. However, traditional approaches to genetic score construction face a range of challenges, including a lack of interpretability, bias towards the disease outcome, and often overfitting to the study they were derived on. Here, we develop an alternative strategy by first generating a genetic pathway function score for oxidative stress (gPFSox) based on the transcriptional activity levels of the oxidative stress response pathway in brain and other tissue types. Then, in the Barwon Infant Study (BIS), a population-based birth cohort (n = 1074), we show that a high gPFSox, indicating reduced ability to counter oxidative stress, is linked to higher autism spectrum disorder risk and higher parent-reported autistic traits at age 4 years, with AOR values (per 2 additional pro-oxidant alleles) of 2.10 (95% CI (1.12, 4.11); p = 0.024) and 1.42 (95% CI (1.02, 2.01); p = 0.041), respectively. Past work in BIS has reported higher prenatal phthalate exposure at 36 weeks of gestation associated with offspring autism spectrum disorder. In this study, we examine combined effects and show a consistent pattern of increased neurodevelopmental problems for individuals with both a high gPFSox and high prenatal phthalate exposure across a range of outcomes, including high gPFSox and high DEHP levels against autism spectrum disorder (attributable proportion due to interaction 0.89; 95% CI (0.62, 1.16); p < 0.0001). The results highlight the utility of this novel functional genetic score and add to the growing evidence implicating gestational phthalate exposure in adverse neurodevelopment.
A Risk Assessment Method of Power Transformer Based on Three-Parameter Interval Grey Number Decision-Making
Authors: Yu Hongbo , Xiong Wei , Xu Kui , Yu Yunwen , Yuan Xufeng , Zou Xiaosong , Xiao Ling
Published: March 2022

In the process of power transformer risk assessment, the loss degree index is difficult to accurately quantify due to the influence of uncertain factors, leading to the deviation of risk judgment. A power transformer risk assessment method based on the three-parameter interval grey number decision-making is proposed. Firstly, the fault probability of the transformer is quantified based on the condition evaluation results. Secondly, considering the uncertainty of DG output and load, the Nataf transform and Cholesky decomposition were used to eliminate the correlation of random variables, and a three-point estimation method combined with a DC cut load model was introduced to calculate the probability distribution of the loss degree caused by the transformer fault. Finally, the origin moment of each order was obtained based on the calculation formula of risk value, and the risk probability distribution was obtained through the Cornish–Fisher series expanding. The decision method of the three-parameter interval grey number distance measure was used to judge the risk grade of the equipment. The results show that the proposed method fully considers the influence of uncertainty on equipment risk judgment, can realize the full use of the equipment risk value interval number to judge the risk, and avoids the decision-making defects of the traditional certain risk quantification method. Meanwhile, the influence of different factors on the risk evaluation results is in line with the actual operation condition of the transformer. The results also verify the effectiveness and accuracy of the proposed method, which provides a new judgment idea for power grid equipment risk quantitative assessment.
Black Carbon Evolution at WMO/GAW Station Mt. Waliguan China and Contribution Area from 1994 to 2017
Authors: Pu Dongyang , Meng Rongqian , Wu Hao , Zhen Fudong
Published: March 2022

Black carbon (BC) aerosol measured at the WMO/GAW Station Mt. Waliguan from 1994 to 2017 has been analyzed. The 24 years long-term results showed that the average annual concentration ranges from 1.9 × 102 ng m−3 to 5.1 × 102 ng m−3 from 2001 to 2012, with a growth rate of 29%. However, the concentration of black carbon decreased from 2012 to 2016, with a decline rate of 64%. The monthly average concentration over the 24 years ranged from 90 ng m−3 to 7.0 × 102 ng m−3, with the peak value occurring in April and the lowest value occurring in November. The diurnal variation presented two peak types in different seasons, the first occurred at 20:00 a.m.~23:00 a.m. in the evening, and another around 06:00 a.m.~08:00 a.m. In addition, we found that the transport of black carbon aerosol is closely related to wind transport. The annual maximum black carbon concentration occurred in the east-northeast (ENE) wind direction, with a value of 4.6 × 102 ng m−3, and the second peak value occurred in the E wind direction, with a value of 3.9 × 102 ng m−3. The black carbon concentration of Waliguan was relatively high under the three wind directions of Northeast (NE), ENE, and east (E), which represented the influence of black carbon aerosol generated by human activities located on the east of the station. The 96-h backward trajectory analysis indicated that the sources in the southwest direction made a greater contribution to the black carbon concentration. the pollutants mainly came from the northwest and west sides according to the analysis of potential sources using the CWT approach. The study of black carbon evolution and contribution area is of great significance to further improve the capacity and level of global climate change research and prediction.
Classification Prediction of PM10 Concentration Using a Tree-Based Machine Learning Approach
Authors: Shaziayani Wan Nur , Ul-Saufie Ahmad Zia , Mutalib Sofianita , Mohamad Noor Norazian , Zainordin Nazatul Syadia
Published: March 2022

The PM10 prediction has received considerable attention due to its harmful effects on human health. Machine learning approaches have the potential to predict and classify future PM10 concentrations accurately. Therefore, in this study, three machine learning algorithms—namely, decision tree (DT), boosted regression tree (BRT), and random forest (RF)—were applied for the prediction of PM10 in Kota Bharu, Kelantan. The results from these three methods were compared to find the best method to predict PM10 concentration for the next day by using the maximum daily data from January 2002 to December 2017. To this end, 80% of the data were used for training and 20% for validation of the models. The performance measure of the PM10 concentration was based on accuracy, sensitivity, specificity, and precision for RF, BRT, and DT, respectively, which indicates that these three models were developed effectively, and they are applicable in the prediction of other atmospheric environmental data. The best model to use in predicting the next day’s PM10 concentration classification was the random forest classifier, with an accuracy of 98.37, sensitivity of 97.19, specificity of 99.55, and precision of 99.54, but the result of the boosted regression tree was substantially different from the RF model, with an accuracy of 98.12, sensitivity of 97.51, specificity of 98.72, and precision of 98.71. The best model can assist local governments in providing early warnings to people who are at risk of acute and chronic health consequences from air pollution.
Impact of Increased Vertical Resolution in WACCM on the Climatology of Major Sudden Stratospheric Warmings
Authors: Chávez Víctor M. , Añel Juan A. , Garcia Rolando R. , Šácha Petr , Torre Laura de la
Published: March 2022

Sudden stratospheric warmings (SSWs) are a major mode of variability of the winter stratosphere. In recent years, climate models have improved their ability to simulate SSWs. However, the representation of the frequency and temporal distribution of SSWs in models depends on many factors and remains challenging. The vertical resolution of a model might be one such factor. Therefore, here we analyse the impact of increased vertical resolution on the simulation of major sudden stratospheric warmings (SSWs) in the Whole Atmosphere Community Climate Model (WACCM). We compare two versions of the model, WACCM3.5 and WACCM4. We find that the frequency of occurrence of SSWs is improved in the newer version and closer to that obtained using reanalysis. Furthermore, simulations with a coupled ocean best reproduce the behaviour of temperature during these events. Increasing vertical resolution increases the number of occurrences; however, it does not produce significantly different results than standard resolution. WACCM4 also does not reproduce vortex split events well, generating far fewer of these than observed. Finally, the ratio between polar vortex splits and displacement events in the model is slightly better for non-ocean-coupled simulations. We conclude that, at least for WACCM4, the use of the high vertical resolution configuration is not cost-effective for the study of SSWs.
A Systematic Approach to Diagnostic Laboratory Software Requirements Analysis
Authors: Krause Thomas , Jolkver Elena , Mc Kevitt Paul , Kramer Michael , Hemmje Matthias
Published: March 2022

Genetics plays an ever-increasing role in medical diagnostics. The requirements for laboratory diagnostics are constantly changing due to new emerging diagnostic procedures, methodologies, devices, and regulatory requirements. Standard software already available for laboratories often cannot keep up with the latest developments or is focused on research rather than process automation. Although the software utilized in diagnostic laboratories is subject to regulatory requirements, there is no well-defined formal procedure for software development. Reference models have been developed to formalize these solutions, but they do not facilitate the initial requirements analysis or the development process itself. A systematic requirements engineering process is however not only essential to ensure the quality of the final product but is also required by regulations such as the European In Vitro Diagnostic Regulation and international standards such as IEC 62304. This paper shows, by example, the systematic requirements analysis of a system for qPCR-based (quantitative polymerase chain reaction) gene expression analysis. Towards this goal, a multi-step research approach was employed, which included literature review, user interviews, and market analysis. Results revealed the complexity of the field with many requirements to be considered for future implementation.
Implant Periapical Lesion: Clinical and Histological Analysis of Two Case Reports Carried Out with Two Different Approaches
Authors: Luongo Roberto , Faustini Fabio , Vantaggiato Alessandro , Bianco Giuseppe , Traini Tonino , Scarano Antonio , Pedullà Eugenio , Bugea Calogero
Published: March 2022

Periapical implantitis (IPL) is an increasingly frequent complication of dental implants. The causes of this condition are not yet entirely clear, although a bacterial component is certainly part of the etiology. In this case series study, two approaches will be described: because of persistent IPL symptoms, a patient had the implant removed and underwent histological analysis after week 6 from implantation. The histomorphometric examination revealed a 35% bone-implant contact area involving the coronal two-thirds of the implant. The apical portion of the fixture on the other hand was affected by an inflammatory process detectable on radiography as a radiolucent area. The presence of a probable root fragment, detectable as an imprecise radiopaque mass in the zone where the implant was later placed, confirms the probable bacterial etiology of this case of IPL. On the other hand, in case number 2, the presence of IPL around the fixture was solved by surgically removing the implant apical third as well as the adjacent tooth apex. It may be concluded from our histological examination that removal of the apical portion of the fixture should be considered an effective treatment for IPL since the remaining implant segment remains optimally osseointegrated and capable of continuing its function as a prosthetic abutment. Careful attention, however, is required at the implantation planning stage to identify in advance any sources of infection in the edentulous area of interest which might compromise the final outcome.
Genome Characterisation of an Isoprene-Degrading Alcaligenes sp. Isolated from a Tropical Restored Forest
Authors: Uttarotai Toungporn , Sutheeworapong Sawannee , Crombie Andrew T. , Murrell J. Colin , Mhuantong Wuttichai , Noirungsee Nuttapol , Wangkarn Sunanta , Bovonsombut Sakunnee , McGenity Terry J. , Chitov Thararat
Published: March 2022

Isoprene is a climate-active biogenic volatile organic compound (BVOC), emitted into the atmosphere in abundance, mainly from terrestrial plants. Soil is an important sink for isoprene due to its consumption by microbes. In this study, we report the ability of a soil bacterium to degrade isoprene. Strain 13f was isolated from soil beneath wild Himalayan cherry trees in a tropical restored forest. Based on phylogenomic analysis and an Average Nucleotide Identity score of >95%, it most probably belongs to the species Alcaligenes faecalis. Isoprene degradation by Alcaligenes sp. strain 13f was measured by using gas chromatography. When isoprene was supplied as the sole carbon and energy source at the concentration of 7.2 × 105 ppbv and 7.2 × 106 ppbv, 32.6% and 19.6% of isoprene was consumed after 18 days, respectively. Genome analysis of Alcaligenes sp. strain 13f revealed that the genes that are typically found as part of the isoprene monooxygenase gene cluster in other isoprene-degrading bacteria were absent. This discovery suggests that there may be alternative pathways for isoprene metabolism.
Trophic Niche Breadth of Falconidae Species Predicts Biomic Specialisation but Not Range Size
Authors: Fargallo Juan A. , Navarro-López Juan , Cantalapiedra Juan L. , Pelegrin Jonathan S. , Hernández Fernández Manuel
Published: March 2022

Trophic niche breadth plays a key role in biogeographic distribution patterns. Theory posits that generalist strategies are favoured in a more heterogeneous set of environments across a spatio-temporal gradient of resources predictability, conferring individuals and species a greater capacity for colonising new habitats and thus expanding their distribution area. Using the family Falconidae (Aves, Falconiformes) as a model study, we tested the prediction that those species with a wider diet spectrum will have larger geographic range sizes and inhabit more biomes. We assessed the relationships between trophic breadth (diet richness and diversity) at different taxonomic resolutions of the prey (class and order), range size and biomic specialisation index (BSI; number of biomes inhabited) for the different species. Despite different diet breadth indexes and taxonomic resolutions defined differently the trophic niche of the clade and species, our findings revealed that trophic breadth was not a good predictor for range size but was for total environmental heterogeneity, with more diet-generalist species occupying a higher number of biomes. Diet breadth at the order taxonomic level showed a higher capacity of predicting BSI than at class level, and can be an important ecological trait explaining biogeographic patterns of the species.
How Do Drugs Affect the Skeleton? Implications for Forensic Anthropology
Authors: Márquez-Grant Nicholas , Baldini Elisa , Jeynes Victoria , Biehler-Gomez Lucie , Aoukhiyad Layla , Passalacqua Nicholas V. , Giordano Gaia , Di Candia Domenico , Cattaneo Cristina
Published: March 2022

Forensic anthropologists rely on a number of parameters when analyzing human skeletal remains to assist in the identification of the deceased, predominantly age-at-death, sex, stature, ancestry or population affinity, and any unique identifying features. During the examination of human remains, it is important to be aware that the skeletal features considered when applying anthropological methods may be influenced and modified by a number of factors, and particular to this article, prescription drugs (including medical and non-medical use) and other commonly used drugs. In view of this, this paper aims to review the medical, clinical and pharmacological literature to enable an assessment of those drug groups that as side effects have the potential to have an adverse effect on the skeleton, and explore whether or not they can influence the estimation of age-at-death, sex and other indicators of the biological profile. Moreover, it may be that the observation of certain alterations or inconsistencies in the skeleton may relate to the use of drugs or medication, and this in turn may help narrow down the list of missing persons to which a set of human remains could belong. The information gathered from the clinical and medical literature has been extracted with a forensic anthropological perspective and provides an awareness on how several drugs, such as opioids, cocaine, corticosteroids, non-steroidal anti-inflammatory drugs, alcohol, tobacco and others have notable effects on bone. Through different mechanisms, drugs can alter bone mineral density, causing osteopenia, osteoporosis, increase the risk of fractures, osteonecrosis, and oral changes. Not much has been written on the influence of drugs on the skeleton from the forensic anthropological practitioner perspective; and this review, in spite of its limitations and the requirement of further research, aims to investigate the current knowledge of the possible effects of both prescription and recreational drugs on bones, contributing to providing a better awareness in forensic anthropological practice and assisting in the identification process of the deceased.
Epigenetic Regulation in Exposome-Induced Tumorigenesis: Emerging Roles of ncRNAs
Authors: Olmedo-Suárez Miguel Ángel , Ramírez-Díaz Ivonne , Pérez-González Andrea , Molina-Herrera Alejandro , Coral-García Miguel Ángel , Lobato Sagrario , Sarvari Pouya , Barreto Guillermo , Rubio Karla
Published: March 2022

Environmental factors, including pollutants and lifestyle, constitute a significant role in severe, chronic pathologies with an essential societal, economic burden. The measurement of all environmental exposures and assessing their correlation with effects on individual health is defined as the exposome, which interacts with our unique characteristics such as genetics, physiology, and epigenetics. Epigenetics investigates modifications in the expression of genes that do not depend on the underlying DNA sequence. Some studies have confirmed that environmental factors may promote disease in individuals or subsequent progeny through epigenetic alterations. Variations in the epigenetic machinery cause a spectrum of different disorders since these mechanisms are more sensitive to the environment than the genome, due to the inherent reversible nature of the epigenetic landscape. Several epigenetic mechanisms, including modifications in DNA (e.g., methylation), histones, and noncoding RNAs can change genome expression under the exogenous influence. Notably, the role of long noncoding RNAs in epigenetic processes has not been well explored in the context of exposome-induced tumorigenesis. In the present review, our scope is to provide relevant evidence indicating that epigenetic alterations mediate those detrimental effects caused by exposure to environmental toxicants, focusing mainly on a multi-step regulation by diverse noncoding RNAs subtypes.
Blockade of TASK-1 Channel Improves the Efficacy of Levetiracetam in Chronically Epileptic Rats
Authors: Kim Ji-Eun , Kang Tae-Cheon
Published: March 2022

Tandem of P domains in a weak inwardly rectifying K+ channel (TWIK)-related acid sensitive K+-1 channel (TASK-1) is an outwardly rectifying K+ channel that acts in response to extracellular pH. TASK-1 is upregulated in the astrocytes (particularly in the CA1 region) of the hippocampi of patients with temporal lobe epilepsy and chronically epilepsy rats. Since levetiracetam (LEV) is an effective inhibitor for carbonic anhydrase, which has a pivotal role in buffering of extracellular pH, it is likely that the anti-epileptic action of LEV may be relevant to TASK-1 inhibition, which remains to be elusive. In the present study, we found that LEV diminished the upregulated TASK-1 expression in the CA1 astrocytes of responders (whose seizure activities were responsive to LEV), but not non-responders (whose seizure activities were not controlled by LEV) in chronically epileptic rats. ML365 (a selective TASK-1 inhibitor) only reduced seizure duration in LEV non-responders, concomitant with astroglial TASK-1 downregulation. Furthermore, ML365 co-treatment with LEV decreased the duration, frequency and severity of spontaneous seizures in non-responders to LEV. To the best of our knowledge, our findings suggest, for the first time, that the up-regulation of TASK-1 expression in CA1 astrocytes may be involved in refractory seizures in response to LEV. This may be a potential target to improve responsiveness to LEV.
EGFR Amplification Is a Phenomenon of IDH Wildtype and TERT Mutated High-Grade Glioma: An Integrated Analysis Using Fluorescence In Situ Hybridization and DNA Methylome Profiling
Authors: Hölzl Dorothee , Hutarew Georg , Zellinger Barbara , Alinger-Scharinger Beate , Schlicker Hans U. , Schwartz Christoph , Sotlar Karl , Kraus Theo F. J.
Published: March 2022

Gliomas are the most common intrinsic brain tumors in adults, and in accordance with their clinical behavior and patients’ outcome, they are graded by the World Health Organization (WHO) classification of brain tumors. One very interesting candidate for targeted tumor therapy may be epidermal growth factor receptor (EGFR) amplification. Here, we performed an integrated comparative analysis of EGFR amplification in 34 glioma samples using standard fluorescence in situ hybridization (FISH) and Illumina EPIC Infinium Methylation Bead Chip and correlated results with molecular glioma hallmarks. We found that the EPIC analysis showed the same power of detecting EGFR amplification compared with FISH. EGFR amplification was detectable in high-grade gliomas (25%). Moreover, EGFR amplification was found to be present solely in IDH wildtype gliomas (26%) and TERT mutated gliomas (27%), occurring independently of MGMT promoter methylation status and being mutually exclusive with 1p/19q codeletion (LOH). In summary, EPIC Bead Chip analysis is a reliable tool for detecting EGFR amplification and is comparable with the standard method FISH. EGFR amplification is a phenomenon of IDH wildtype TERT mutated high-grade gliomas.
Multi-Layered Human Blood Vessels-on-Chip Design Using Double Viscous Finger Patterning
Authors: Delannoy Elise , Tellier Géraldine , Cholet Juliette , Leroy Alice M. , Treizebré Anthony , Soncin Fabrice
Published: March 2022

Blood vessel-on-a-chip models aim at reproducing vascular functions. However, very few efficient methods have been designed to address the need for biological replicates in medium- to high-throughput screenings. Here, vessels-on-chip were designed in polydimethylsiloxane-glass chips using the viscous finger patterning technique which was adapted to create channels with various internal diameters inside a collagen solution and to simultaneously seed cells. This method was refined to create blood vessels composed of two concentric, distinct, and closely appositioned layers of human endothelial and perivascular cells arranged around a hollow lumen. These approaches allowed the formation of structurally correct blood vessels-on-chips which were constituted of either only endothelial cells or of both cell types in order to distinguish the vascular barrier reactivity to drugs in the presence or not of perivascular cells. The established vessels showed a tight vascular barrier, as assessed by immunostaining of the adherens junctions, and were reactive to the natural vasopermeant thrombin and to inflammatory cytokines. The presence of perivascular cells markedly increased the tightness of the vascular barrier and lowered its response to thrombin. The design allowed us to simultaneously challenge in real-time several tens of 3D-reconstituted, multicellular blood vessels in a standard multiwell plate format suitable for high-throughput drug screening.
In Vitro Effects of Mitochondria-Targeted Antioxidants in a Small-Cell Carcinoma of the Ovary of Hypercalcemic Type and in Type 1 and Type 2 Endometrial Cancer
Authors: Castelôa Mariana , Moreira-Pinto Beatriz , Benfeito Sofia , Borges Fernanda , Fonseca Bruno M. , Rebelo Irene
Published: March 2022

Small-cell carcinoma of the ovary of hypercalcemic type (SCCOHT) and endometrial cancer from type 1 and type 2 are gynecological tumors that affect women worldwide. The treatment encompasses the use of cytotoxic drugs that are nonspecific and inefficient. “Mitocans”, a family of drugs that specifically target tumor cells’ mitochondria, might be a solution, as they conjugate compounds, such as antioxidants, with carriers, such as lipophilic cations, that direct them to the mitochondria. In this study, caffeic acid was conjugated with triphenylphosphonium (TPP), 4-picolinium, or isoquinolinium, forming 3 new compounds (Mito6_TPP, Mito6_picol., and Mito6_isoq.) that were tested on ovarian (COV434) and endometrial (Hec50co and Ishikawa) cancer cells. The results of MTT and neutral red assays suggested a time- and concentration-dependent decrease in cell viability in all tumor cell lines. The presence of apoptosis was indicated by the Giemsa and Höechst staining and by the decrease in mitochondrial membrane potential. The measurement of intracellular reactive oxygen species demonstrated the antioxidant properties of these compounds, which might be related to cell death. Generally, Mito6_TPP was more active at lower concentrations than Mito6_picol. or Mito6_isoq., but was accompanied by more cytotoxic effects, as shown by the lactate dehydrogenase release. Non-tumorous cells (HFF-1) showed no changes after treatment. This study assessed the potential of these compounds as anticancer agents, although further investigation is needed.
Imprinted Photonic Crystal-Film-Based Smartphone-Compatible Label-Free Optical Sensor for SARS-CoV-2 Testing
Authors: Kawasaki Daiki , Yamada Hirotaka , Sueyoshi Kenji , Hisamoto Hideaki , Endo Tatsuro
Published: March 2022

The coronavirus disease (COVID-19) caused by SARS-CoV-2 has caused a global pandemic. To manage and control the spread of the infection, it is crucial to develop and implement technologies for the early identification of infected individuals and rapid informatization in communities. For the realization of such a technology, a widely available and highly usable sensor for sensitive and specific assay of the virus plays a fundamental role. In this study, we developed an optical sensor based on an imprinted photonic crystal film (IPCF) for quick, simple, and cost-effective detection of SARS-CoV-2 spike protein in artificial saliva. Our IPCF sensor enabled label-free and highly sensitive detection with a smartphone-equipped optical setup. The IPCF surface was functionalized with an anti-SARS-CoV-2 spike protein antibody for immunoassay. We evaluated the specificity and sensitivity of the IPCF sensor for quantitative detection of the spike protein in artificial saliva using simple reflectometry with a spectrometer-equipped optical setup. Specific and quantitative detection of the spike protein was successfully achieved, with a low detection limit of 429 fg/mL. In the demonstration of reflectometric detection with a smartphone-equipped setup, the sensitivity was comparable with that with a spectrometer-equipped setup. The test result is returned immediately and can be saved to cloud storage. In addition, it costs less than USD 1 for one IPCF to be used for diagnosis. Thus, the developed IPCF has the potential to realize a widely available and highly usable sensor.