bluuebunny/crossref_metadata_2025 huggingface dataset:
A space-time mosaic of seawater carbonate chemistry conditions in the north-shore Moorea coral reef system
Authors: Dişa Deniz , Münnich Matthias , Vogt Meike , Gruber Nicolas
Published: December 2022

The interplay between ocean circulation and coral metabolism creates highly variable biogeochemical conditions in space and time across tropical coral reefs. Yet, relatively little is known quantitatively about the spatiotemporal structure of these variations. To address this gap, we use the Coupled Ocean Atmosphere Wave and Sediment Transport (COAWST) model, to which we added the Biogeochemical Elemental Cycling (BEC) model computing the biogeochemical processes in the water column, and a coral polyp physiology module that interactively simulates coral photosynthesis, respiration and calcification. The coupled model, configured for the north-shore of Moorea Island, successfully simulates the observed (i) circulation across the wave regimes, (ii) magnitude of the metabolic rates, and (iii) large gradients in biogeochemical conditions across the reef. Owing to the interaction between coral net community production (NCP) and coral calcification, the model simulates distinct day versus night gradients, especially for pH and the saturation state of seawater with respect to aragonite (Ωα). The strength of the gradients depends non-linearly on the wave regime and the resulting residence time of water over the reef with the low wave regime creating conditions that are considered as “extremely marginal” for corals. With the average water parcel passing more than twice over the reef, recirculation contributes further to the accumulation of these metabolic signals. We find diverging temporal and spatial relationships between total alkalinity (TA) and dissolved inorganic carbon (DIC) (≈ 0.16 for the temporal vs. ≈ 1.8 for the spatial relationship), indicating the importance of scale of analysis for this metric. Distinct biogeochemical niches emerge from the simulated variability, i.e., regions where the mean and variance of the conditions are considerably different from each other. Such biogeochemical niches might cause large differences in the exposure of individual corals to the stresses associated with e.g., ocean acidification. At the same time, corals living in the different biogeochemical niches might have adapted to the differing conditions, making the reef, perhaps, more resilient to change. Thus, a better understanding of the mosaic of conditions in a coral reef might be useful to assess the health of a coral reef and to develop improved management strategies.
Numerical and experimental research of helical gear contact stress considering the influence of friction
Authors: Yang Xiaopeng , Yin Shunliang , Chen Yong , Zhang Yong , Zhang Shuang , Wu Yimin
Published: December 2022

Helical gears are widely used in various mechanical transmissions. Thus, analysing gear contact stresses is crucial to improve the fatigue limit and life of gears. In this paper, the contact stress of helical gear is analyzed by finite element simulation and experimental verification. The effect of coefficient of friction on the contact stress of helical gears is analysed, with given coefficients of friction. The following conclusions can be drawn through simulation and test. 1) The contact stress increases with the coefficient of friction in the early stage of meshing. 2) The contact stress decreases with the increase in the coefficient of friction in the late stage of meshing. 3) The gear contact fatigue test shows that the location of fatigue pitting on the tooth surface is consistent with that of the simulated maximum stress point. The friction-reducing coating on the gear surface can decrease the coefficient of friction between the tooth surfaces and thus effectively improve the gear contact fatigue life.
Impact of carbendazim on cellular growth, defence system and plant growth promoting traits of Priestia megaterium ANCB-12 isolated from sugarcane rhizosphere
Authors: Sharma Anjney , Song Xiu-Peng , Singh Rajesh Kumar , Vaishnav Anukool , Gupta Saurabh , Singh Pratiksha , Guo Dao-Jun , Verma Krishan K. , Li Yang-Rui
Published: December 2022

Agrochemicals are consistently used in agricultural practices to protect plants from pathogens and ensure high crop production. However, their overconsumption and irregular use cause adverse impacts on soil flora and non-target beneficial microorganisms, ultimately causing a hazard to the ecosystem. Taking this into account, the present study was conducted to determine the high dosage of fungicide (carbendazim: CBZM) effects on the rhizobacteria survival, plant growth promoting trait and reactive oxygen species (ROS) scavenging antioxidant enzyme system. Thus, a multifarious plant growth promoting rhizobacteria (PGPR) isolate, ANCB-12, was obtained from the sugarcane rhizosphere through an enrichment technique. The taxonomic position of the isolated rhizobacteria was confirmed through 16S rRNA gene sequencing analysis as Priestia megaterium ANCB-12 (accession no. ON878101). Results showed that increasing concentrations of fungicide showed adverse effects on rhizobacterial cell growth and survival. In addition, cell visualization under a confocal laser scanning microscope (CLSM) revealed more oxidative stress damage in the form of ROS generation and cell membrane permeability. Furthermore, the increasing dose of CBZM gradually decreased the plant growth promoting activities of the rhizobacteria ANCB-12. For example, CBZM at a maximum 3,000 μg/ml concentration decreases the indole acetic acid (IAA) production by 91.6%, ACC deaminase by 92.3%, and siderophore production by 94.1%, respectively. Similarly, higher dose of fungicide enhanced the ROS toxicity by significantly (p < 0.05) modulating the stress-related antioxidant enzymatic biomarkers in P. megaterium ANCB-12. At a maximum 3,000 μg/ml CBZM concentration, the activity of superoxide dismutase (SOD) declined by 82.3%, catalase (CAT) by 61.4%, glutathione peroxidase (GPX) by 76.1%, and glutathione reductase (GR) by 84.8%, respectively. The results of this study showed that higher doses of the fungicide carbendazim are toxic to the cells of plant-beneficial rhizobacteria. This suggests that a recommended dose of fungicide should be made to lessen its harmful effects.
Genome-based taxonomic rearrangement of Oceanobacter-related bacteria including the description of Thalassolituus hydrocarbonoclasticus sp. nov. and Thalassolituus pacificus sp. nov. and emended description of the genus Thalassolituus
Authors: Dong Chunming , Wei Lin , Wang Jianning , Lai Qiliang , Huang Zhaobin , Shao Zongze
Published: December 2022

Oceanobacter-related bacteria (ORB) are a group of oligotrophic marine bacteria play an underappreciated role in carbon cycling. They have been frequently described as one of the dominant bacterial groups with a wide distribution in coastal and deep seawater of global oceans. To clarify their taxonomic affiliation in relation to alkane utilization, phylogenomic and comparative genomics analyses were performed based on currently available genomes from GenBank and four newly isolated strains, in addition to phenotypic and chemotaxonomic characteristics. Consistently, phylogenomic analysis robustly separated them into two groups, which are accordingly hydrocarbon-degrading (HD, Thalassolituus and Oleibacter) and non-HD (NHD, Oceanobacter). In addition, the two groups can also be readily distinguished by several polyphasic taxonomic characteristics. Furthermore, both AAI and POCP genomic indices within the HD group support the conclusion that the members of the genus Oleibacter should be transferred into the genus Thalassolituus. Moreover, HD and NHD bacteria differed significantly in terms of genome size, G + C content and genes involved in alkane utilization. All HD bacteria contain the key gene alkB encoding an alkane monooxygenase, which can be used as a marker gene to distinguish the members of closely related genera Oceanobacter and Thalassolituus. Pangenome analysis revealed that the larger accessory genome may endow Thalassolituus with the flexibility to cope with the dynamics of marine environments and thrive therein, although they possess smaller pan, core- and unique-genomes than Oceanobacter. Within the HD group, twelve species were clearly distinguished from each other by both dDDH and ANI genomic indices, including two novel species represented by the newly isolated strains alknpb1M-1T and 59MF3M-4T, for which the names Thalassolituus hydrocarbonoclasticus sp. nov. and Thalassolituus pacificus sp. nov. are proposed. Collectively, these findings build a phylogenetic framework for the ORB and contribute to understanding of their role in marine carbon cycling.
Comparison of coated and uncoated trace elements on growth performance, apparent digestibility, intestinal development and microbial diversity in growing sheep
Authors: Zhou Jia , Ren Yifan , Wen Xiao , Yue Shuangming , Wang Zhisheng , Wang Lizhi , Peng Quanhui , Hu Rui , Zou Huawei , Jiang Yahui , Hong Qionghua , Xue Bai
Published: December 2022

The suitable supplement pattern affects the digestion and absorption of trace minerals by ruminants. This study aimed to compare the effects of coated and uncoated trace elements on growth performance, apparent digestibility, intestinal development and microbial diversity in growing sheep. Thirty 4-month-old male Yunnan semi-fine wool sheep were randomly assigned to three treatments (n = 10) and fed with following diets: basal diet without adding exogenous trace elements (CON), basal diet plus 400 mg/kg coated trace elements (CTE, the rumen passage rate was 65.87%) and basal diet plus an equal amount of trace elements in uncoated form (UTE). Compared with the CON group, the average daily weight gain and apparent digestibility of crude protein were higher (P < 0.05) in the CTE and UTE groups, while there was no difference between the CTE and UTE groups. The serum levels of selenium, iodine and cobalt were higher (P < 0.05) in the CTE and UTE groups than those in the CON group, the serum levels of selenium and cobalt were higher (P < 0.05) in the CTE group than those in the UTE group. Compared with the CON and UTE groups, the villus height and the ratio of villus height to crypt depth in duodenum and ileum were higher (P < 0.05) in the CTE groups. The addition of trace minerals in diet upregulated most of the relative gene expression of Ocludin, Claudin-1, Claudin-2, ZO-1, and ZO-2 in the duodenum and jejunum and metal ion transporters (FPN1 and ZNT4) in small intestine. The relative abundance of the genera Christensenellaceae R-7 group, Ruminococcus 1, Lachnospiraceae NK3A20 group, and Ruminococcaceae in ileum, and Ruminococcaceae UCG-014 and Lactobacillus in colon was higher in the CTE group that in the CON group. These results indicated that dietary trace mineral addition improved the growth performance and intestinal development, and altered the structure of intestinal bacteria in growing sheep. Compared to uncoated form, offering trace mineral elements to sheep in coated form had a higher absorption efficiency, however, had little effect on improving growth performance of growing sheep.
Prevalence and risk factors of sarcopenia without obesity and sarcopenic obesity among Chinese community older people in suburban area of Shanghai: A cross-sectional study
Authors: Lu Linqian , He Xiangfeng , Song Yanping , Zhuang Min , Wu Xie , Chen Nan
Published: December 2022

Objectives

The aim of the present study was to explore the prevalence and risk factors of sarcopenia without obesity (S) and sarcopenic obesity (SO) among community-dwelling older people in the Chongming District of Shanghai, China, according to the Asian Working Group for Sarcopenia (AWGS) 2019 Consensus as the diagnostic criteria of sarcopenia.

Methods

In this cross-sectional study, a total of 1,407 subjects aged ≥65 years were included, where the mean age of the subjects was 71.91 ± 5.59 years and their mean body mass index (BMI) was 24.65 ± 3.32 kg/m2. According to the Asian Working Group for Sarcopenia (AWGS) 2019 Consensus, sarcopenia was defined as a low appendicular skeletal muscle mass index (≤7.0 kg/m2 in males and ≤5.7 kg/m2 in females), decreased handgrip strength (<28.0 kg in males and <18.0 kg in females), and/or low gait speed (<1.0 m/s) or poor 5-time chair stand test (5CST) (≥12s). The SO met both the diagnostic criteria for sarcopenia and obesity, meanwhile obesity was defined as an increased percentage of body fat (PBF) (≥25% in males and ≥35% in females). Univariate and multiple logistic regression analyses were performed to explore the risk factors of both S and SO.

Results

The prevalence of S and SO was 9.74% (M: 9.29%, F: 10.05%) and 9.95% (M: 13.94%, F: 7.14%). Lower BMI (OR = 0.136, 95% CI: 0.054–0.340, p < 0.001), lower hip circumference (OR = 0.858, 95% CI: 0.816–0.903, p < 0.001), farming (OR = 1.632, 95% CI: 1.053–2.530, p = 0.028), higher high-density lipoprotein cholesterol (HDL-C) level (OR = 2.235, 95% CI: 1.484–3.367, p < 0.001), and a sleep duration <7 h (OR = 0.561, 95% CI: 0.346–0.909, p = 0.019) were risk factors for S. While aging (70–74 y, OR = 1.923, 95% CI: 1.122–3.295, p = 0.017; 75–79 y, OR = 3.185, 95% CI: 1.816–5.585, p < 0.001; ≥80 y, OR = 7.192, 95% CI: 4.133–12.513, p < 0.001), male (OR = 1.981, 95% CI: 1.351–2.904, p < 0.001), higher BMI (OR = 4.865, 95% CI: 1.089–21.736, p = 0.038), higher monocyte level (OR = 4.203, 95% CI: 1.340–13.181, p = 0.014), and a sleep duration >9 h (OR = 1.881, 95% CI: 1.117–3.166, p = 0.017) were risk factors for SO.

Conclusion

Our study showed the high prevalence of S and SO among community-dwelling older people in the Chongming District. The SO was more prevalent in males. Behavioral factors and lifestyle (such as farming and sleep duration) were associated more with the development of S, while age and male gender were associated more with the development of SO.
Induced pluripotent stem cell-derived and directly reprogrammed neurons to study neurodegenerative diseases: The impact of aging signatures
Authors: Aversano Simona , Caiazza Carmen , Caiazzo Massimiliano
Published: December 2022

Many diseases of the central nervous system are age-associated and do not directly result from genetic mutations. These include late-onset neurodegenerative diseases (NDDs), which represent a challenge for biomedical research and drug development due to the impossibility to access to viable human brain specimens. Advancements in reprogramming technologies have allowed to obtain neurons from induced pluripotent stem cells (iPSCs) or directly from somatic cells (iNs), leading to the generation of better models to understand the molecular mechanisms and design of new drugs. Nevertheless, iPSC technology faces some limitations due to reprogramming-associated cellular rejuvenation which resets the aging hallmarks of donor cells. Given the prominent role of aging for the development and manifestation of late-onset NDDs, this suggests that this approach is not the most suitable to accurately model age-related diseases. Direct neuronal reprogramming, by which a neuron is formed via direct conversion from a somatic cell without going through a pluripotent intermediate stage, allows the possibility to generate patient-derived neurons that maintain aging and epigenetic signatures of the donor. This aspect may be advantageous for investigating the role of aging in neurodegeneration and for finely dissecting underlying pathological mechanisms. Here, we will compare iPSC and iN models as regards the aging status and explore how this difference is reported to affect the phenotype of NDD in vitro models.
Magnetic resonance imaging assessment of the therapeutic effect of combined electroacupuncture and stem cells in acute peripheral nerve injury
Authors: Chen Yueyao , Pan Zhongxian , Meng Fanqi , Yu Xuewen , Xu Qian , Huang Leyu , Liang Qiumei , Wu Yanglei , Lin Xiaofeng
Published: December 2022

Objectives: This study aimed to evaluate the therapeutic effect of a combination of Bone Mesenchymal stem cells (BMSCs) transplantation and Electroacupuncture (EA) for acute sciatic nerve injury in rats using magnetic resonance.

Methods: Ninety-two male adult healthy Sprague-Dawley rats were randomly divided into the EA+BMSCs group, EA group, MSCs group, and PBS group (control). Electroacupuncture was performed on a rat receiving EA treatment at Huantiao (GB30) and Zusanli (ST36). T2 values and diffusion tensor imaging (DTI) derived from multiparametric magnetic resonance imaging (MRI), histological assessments, and immunohistochemistry was used to monitor nerve regeneration. Walking track analysis was used to assess nerve functional recovery. Repeated-measures one-way analysis of variance was used to evaluate the significance of T2, DTI, and SFI values among the four groups. One-way analysis of variance was used for comparing the histological characteristics. Bonferroni test was used for multiple pairwise comparisons at each time point.

Results: In terms of FA, the EA+BMSCs and EA groups had faster recovery than PBS (control) in all time points after surgery, and the EA+BMSCs group recovered better than the BMSCs group at 3 weeks (P ≤ 0.008). FA values were higher in the EA group than in the BMSCs group at 4 weeks (P ≤ 0.008). In terms of RD, the EA+BMSCs group recovered better than the BMSCs group at 2 and 4 weeks (P ≤ 0.008). Immunofluorescence staining for axon guidance molecule netrin-1 revealed that it was significantly higher in the EA+BMSCs subgroup and EA subgroup than it was in the control (PBS) subgroup at 1–3 weeks (P < 0.001). Immunofluorescence staining for S100 showed the continuity of nerve fibers recovered more quickly in the EA+BMSCs subgroup than in the BMSCs subgroup.

Conclusion: Our research revealed that a combination of MSCs and EA can provide both topological and biomolecular guidance to promote axonal extension, myelin regeneration, and functional recovery after PNI. EA not only promotes nerve repair on its own, but also enhanced the beneficial effects of stem cell treatment and the secretion of netrin 1, a guidance regeneration factor, and promotes the orderly growth of nerve fibers. These PNI repairs could be monitored non-invasively and in situ by MRI. The FA and RD values derived from MRI could be sensitive biomarkers to reflect the PNI repair process.
Functions and dysfunctions of oligodendrocytes in neurodegenerative diseases
Authors: Han Seungwan , Gim Yunho , Jang Eun-Hae , Hur Eun-Mi
Published: December 2022

Neurodegenerative diseases (NDDs) are characterized by the progressive loss of selectively vulnerable populations of neurons, which is responsible for the clinical symptoms. Although degeneration of neurons is a prominent feature that undoubtedly contributes to and defines NDD pathology, it is now clear that neuronal cell death is by no means mediated solely by cell-autonomous mechanisms. Oligodendrocytes (OLs), the myelinating cells of the central nervous system (CNS), enable rapid transmission of electrical signals and provide metabolic and trophic support to neurons. Recent evidence suggests that OLs and their progenitor population play a role in the onset and progression of NDDs. In this review, we discuss emerging evidence suggesting a role of OL lineage cells in the pathogenesis of age-related NDDs. We start with multiple system atrophy, an NDD with a well-known oligodendroglial pathology, and then discuss Alzheimer’s disease (AD) and Parkinson’s disease (PD), NDDs which have been thought of as neuronal origins. Understanding the functions and dysfunctions of OLs might lead to the advent of disease-modifying strategies against NDDs.
The clinical significance of peripheral blood cell ratios in patients with intracranial aneurysm
Authors: Kim Hyun Kyung , Lee Kee Ook , Oh Seung-Hun , Lee Kyung-Yul , Choo Seung-Wook , Kim Ok Joon , Kim Tae Gon , Kim Sang-Heum , Na Sang-Jun , Heo Ji Hoe
Published: December 2022

Background and objective

Inflammation is an important factor in the development of aneurysm, and has been identified as a key characteristic predictive of rupture of intracranial aneurysm (IA). However, the role of inflammatory peripheral blood cell ratios in patients with IA has not been well delineated.

Methods

A total of 1,209 patients, including 1,001 with unruptured IA and 208 with ruptured IA, were enrolled in this study. Neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte ratio (PLR), platelet-to-neutrophil ratio (PNR), and platelet-to-white-blood-cell ratio (PWR) were compared between ruptured and unruptured IA.

Results

Compared with the ruptured IA group, the unruptured IA group had higher PNR {median, 65.96 [interquartile range (IQR) 48.95–85.05] vs. 37.78 (IQR, 23.17–54.05); p < 0.001} and PWR [median, 36.89 (IQR 29.38–44.56) vs. 22.39 (IQR, 16.72–29.29); p < 0.001]. In multivariate analysis, PNR and PWR were independently associated with ruptured IA (p = 0.001 and p < 0.001, respectively). Unruptured IA subgroup analyses according to the PHASES scores showed that a higher PHASES score was associated with significantly higher NLR and erythrocyte sedimentation rate (p < 0.001 and p = 0.025) and lower PNR and PWR (p < 0.001 and p = 0.007).

Conclusions

We demonstrated that lower PNR and PWR levels are associated with ruptured IA and a higher PHASES score. Unlike many other inflammatory markers and bioassays, peripheral blood cell ratios are inexpensive and readily available biomarkers that may be useful for risk stratification in patients with cerebral aneurysm. However, a long-term prospective study is needed to clarify this matter.
Identification of candidate genes associated with clinical onset of Alzheimer’s disease
Authors: Liao Wang , Luo Haoyu , Ruan Yuting , Mai Yingren , Liu Chongxu , Chen Jiawei , Yang Shaoqing , Xuan Aiguo , Liu Jun
Published: December 2022

Background and objective

Alzheimer’s disease (AD) is the most common type of dementia, with its pathology like beta-amyloid and phosphorylated tau beginning several years before the clinical onset. The aim is to identify genetic risk factors associated with the onset of AD.

Methods

We collected three microarray data of post-mortem brains of AD patients and the healthy from the GEO database and screened differentially expressed genes between AD and healthy control. GO/KEGG analysis was applied to identify AD-related pathways. Then we distinguished differential expressed genes between symptomatic and asymptomatic AD. Feature importance with logistic regression analysis is adopted to identify the most critical genes with symptomatic AD.

Results

Data was collected from three datasets, including 184 AD patients and 132 healthy controls. We found 66 genes to be differently expressed between AD and the control. The pathway enriched in the process of exocytosis, synapse, and metabolism and identified 19 candidate genes, four of which (VSNL1, RTN1, FGF12, and ENC1) are vital.

Conclusion

VSNL1, RTN1, FGF12, and ENC1 may be the essential genes that progress asymptomatic AD to symptomatic AD. Moreover, they may serve as genetic risk factors to identify high-risk individuals showing an earlier onset of AD.
Neural correlates of food labels on brand, nature, and nutrition: An fMRI meta-analysis
Authors: Yeung Andy Wai Kan
Published: December 2022

Eating is an essential act of our everyday life, and it involves complicated cognitive appraisal and gustatory evaluation. This study meta-analyzed the functional magnetic resonance imaging (fMRI) studies about food labels on brand, nature and nutrition. Web of Science Core Collection (WoS), Scopus, and PubMed were queried to identify human fMRI studies written in English and published in peer-reviewed journals and used taste or food related labels. Studies were excluded if they reported no results from taste/food related stimuli versus control, no task-based fMRI results, or no results from whole-brain analysis. Nineteen studies entered the analysis. Results for the meta-analysis on food nutrition revealed that the precuneus on the right hemisphere was significantly activated, a brain region related to internal mentation of self-consciousness and nutritional evaluation. Results for the overall analysis on all 19 studies, the analysis on food brand, and the analysis on food nature revealed no significant brain regions. Food nutrition labels were generally processed by brain regions related to internal mentation of self-consciousness and nutritional evaluation. However, the neural correlates of labels of food brand and food nature were inconsistent across studies. More future studies are needed to better understand the cognitive processing of different kinds of food labels in our brain.
Succinylation and redox status in cancer cells
Authors: Dai Xiaofeng , Zhou Yanyan , Han Fei , Li Jitian
Published: December 2022

Succinylation is a post-translational modification (PTM) event that associates metabolic reprogramming with various pathological disorders including cancers via transferring a succinyl group to a residue of the target protein in an enzymic or non-enzymic manner. With our incremental knowledge on the roles of PTM played in tumor initiation and progression, relatively little has been focused on succinylation and its clinical implications. By delineating the associations of succinylation with cancer hallmarks, we identify the, in general, promotive roles of succinylation in manifesting cancer hallmarks, and conceptualize two working modes of succinylation in driving oncogenic signaling, i.e., via altering the structure and charge of target proteins towards enhanced stability and activity. We also characterize succinylation as a reflection of cellular redox homeostatic status and metabolic state, and bring forth the possible use of hyper-succinylated genome for early cancer diagnosis or disease progression indication. In addition, we propose redox modulation tools such as cold atmospheric plasma as a promising intervention approach against tumor cells and cancer stemness via targeting the redox homeostatic environment cells established under a pathological condition such as hypoxia. Taken together, we emphasize the central role of succinylation in bridging the gap between cellular metabolism and redox status, and its clinical relevance as a mark for cancer diagnosis as well as a target in onco-therapeutics.
Assessment of visceral pain with special reference to chronic pancreatitis
Authors: Kuhlmann Louise , Olesen Søren Schou , Drewes Asbjørn Mohr
Published: December 2022

A thorough pain assessment is of utmost importance when managing pain in clinical practice as it is the foundation for defining pain in need of treatment, either interventional or pharmacological. Pain characteristics can also guide interventional strategies and help evaluate the effect of treatment. In research settings, standardized pain assessment is crucial to improve comparability across studies and facilitate meta-analysis. Due to the importance of thorough visceral pain assessment, this manuscript describes the key elements of pain evaluation focusing on chronic pancreatitis. Most studies in pain assessment have focused on somatic pain, and although chronic pain often shares characteristics between etiologies, some differences must be addressed when assessing visceral pain. Especially differences between somatic and visceral pain are apparent, where visceral pain is diffuse and difficult to localize, with referred pain aspects and often autonomic symptoms dominating the clinical picture. These aspects need to be incorporated into the pain assessment instrument. The manuscript will discuss the different ways of assessing pain, including unidimensional measurement scales, multidimensional questionnaires, and quantitative sensory testing. The advantages and challenges linked to the different methods will be evaluated.
Autotoxicity in Panax notoginseng of root exudatesand their allelochemicals
Authors: Xiang Wei , Chen Jianhua , Zhang Fengyuan , Huang Rongshao , Li Liangbo
Published: December 2022

The growth of Panax notoginseng (Burk.) F. H. Chen is frequently hindered due to replanting failure. In the present study, the objective is to determine whether root exudates from P. notoginseng have autotoxicity and identification of allelochemicals from root exudates or rhizosphere soil. We investigated autotoxicity in P. notoginseng using seedling emergence bioassays and hydroponic culture. The allelochemicals in the soils and root exudates were identified with GC-MS, and the autotoxicity of the identified key allelochemicals was investigated by bioassay. The results showed that the root exudates, and extracts from consecutively cultivated soils also showed significant autotoxicity against seedling emergence and growth. In the non-renewed culture solution without activated charcoal (AC), the fresh and dry mass of P. notoginseng tubers of roots was reduced by about half compared to the addition with AC. A total of 44 different components from all samples were defined by GC-MS analyses. Furthermore, the results of multiple statistical analysis showed a t the difference among cultivated soil, uncultivated soil and root exudates. Bioassay of the identified allelochemicals revealed that benzoic acid, phthalic acid, palmitic acid, and stearic acid significantly affected the root growth of P. notoginseng. These substances at 100 μM more significantly decreased the number of lateral roots. Our results demonstrated that autotoxicity results in replant failure of P. notoginseng.
Syringic acid from rice roots inhibits soil nitrification and N2O emission under red and paddy soils but not a calcareous soil
Authors: Lu Yufang , Hua Yao , Lv Na , Zu Weijun , Kronzucker Herbert J. , Dong Gangqiang , Shi Weiming
Published: December 2022

Syringic acid (SA) is a novel biological nitrification inhibitor (BNIs) discovered in rice root exudates with significant inhibition of Nitrosomonas strains. However, the inhibitory effect of SA on nitrification and nitrous oxide (N2O) emissions in different soils and the environmental factors controlling the degree of inhibition have not been studied. Using 14-day microcosm incubation, we investigated the effects of different concentrations of SA on nitrification activity, abundance of ammonia-oxidizing microorganisms, and N2O emissions in three typical agricultural soils. The nitrification inhibitory efficacy of SA was strongest in acidic red soil, followed by weakly acidic paddy soil, with no significant effect in an alkaline calcareous soil. Potential nitrification activity (PNA) were also greatly reduced by SA additions in paddy and red soil. Pearson correlation analysis showed that the inhibitory efficacy of SA might be negatively correlated with soil pH and positively correlated with clay percentage. SA treatments significantly reduced N2O emissions by 69.1-79.3% from paddy soil and by 40.8%-46.4% from red soil, respectively, but no effect was recorded in the calcareous soil. SA addition possessed dual inhibition of both ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB) abundance in paddy and red soil. Structural equation modelling revealed that soil ammonium (NH4+) and dissolved organic carbon content (DOC) were the key variables explaining AOA and AOB abundance and subsequent N2O emissions. Our results support the potential for the use of the BNI SA in mitigating N2O emissions and enhancing N utilization in red and paddy soils.
Back to basics: A re-evaluation of the relevance of imprinting in the genesis of Bowlby’s attachment theory
Authors: Robledo Juan-Pablo , Cross Ian , Boada-Bayona Luisa , Demogeot Nadine
Published: December 2022

Attachment theory is one of the key theoretical constructs that underpin explorations of human bonding, taking its current form in John Bowlby’s amalgamation of ideas from psychoanalysis, developmental psychology and ethology. Such a period of interdisciplinary exchange, and Bowlby’s interest in Lorenz’ concept of imprinting in particular, have been subject to rather historical and biographical studies, leaving a fine-grained theoretical scrutiny of the exact relationship between imprinting and attachment still pending. This paper attempts to remedy such an omission by exploring the relationships between these two constructs. It critically reviews the theories of imprinting in general, of human imprinting in particular, and of attachment; analysis of the links between these processes bring to the foreground the distinction between supra-individual vs. individual aspects of bonding, the relevance of ‘proto-attachment’ phases before ‘proper’ Bowlbyan attachment is attained, and the role of communicative signals during such early phases. The paper outlines potential benefits of considering such elements in the study of early social cognition, particularly in respect of the study of the gaze and the infant-directed communicative register.
Aptitude and experience as predictors of grammatical proficiency in adult Greek-English bilinguals
Authors: Prela Leonarda , Llompart Miquel , Dąbrowska Ewa
Published: December 2022

It has been shown that individuals exhibit great variability in second language (L2) ultimate attainment. Some speakers reach native-like proficiency, others only achieve a rudimentary command and many lie in the middle. Individual differences research has partly attributed different degrees of L2 attainment to (language) aptitude. Initially considered irrelevant for first language (L1) acquisition, aptitude was viewed as a compensatory ability for adults’ disadvantage in L2 learning. In this line of thought, adults and children are viewed as fundamentally different and rely on different language learning mechanisms. However, aptitude might not be so irrelevant for the L1. Together with input the two factors are found to account for individual differences not only in L2 but also L1 development. Recent research has specifically shown that native grammatical attainment may be modulated by aptitude and input. In this respect, the aim of the current study is to examine the effects of these two predictors (namely input and aptitude) on both L1 and L2 grammatical attainment in the same speakers. Our participants (N = 75) were all native speakers of Greek who learned English as a foreign language in their home country and immigrated to the United Kingdom in adulthood (mean age of arrival = 27.3, SD = 6.4). Grammatical proficiency was measured through a grammaticality judgement task administered in both the L1 and the L2. Aptitude was measured through the Sentence Pairs task (based on the Words In Sentences test from the MLAT battery). Amount of input was measured using the traditional measure, length of residence (LoR) and a new cumulative measure that spanned across the participants’ life. The two measures were pitted against each other in the analysis. We found robust effects of aptitude in both the L1 and the L2, with the effect being even stronger for the L1. As expected, our new cumulative measure of exposure proved to be a better predictor of individual differences in grammatical proficiency. Last but not least, the effects of input were larger for the L2 than the L1.
Effects of psychological interventions on high sports performance: A systematic review
Authors: Reyes-Bossio Mario , Corcuera-Bustamante Santiago , Veliz-Salinas Giancarlo , Villas Boas Junior Marcelo , Delgado-Campusano Mariel , Brocca-Alvarado Paul , Caycho-Rodríguez Tomás , Casas-Apayco Leslie , Tutte-Vallarino Veronica , Carbajal-León Carlos , Brandão Regina
Published: December 2022

Introduction

Intervention programs in sports psychology aid to modify the thoughts and behaviors of athletes in order to improve their performance in sports settings. For high-performance athletes, these interventions are very relevant, given that they constantly face pressure towards obtaining sporting achievements.

Methods

This systematic review aims to analyze the scientific articles between 2010 and 2020 that evaluated the effect of psychological interventions on high-performance athletes. In the search procedure, nine studies were selected, the most studied variables were psychological skills, psychological flexibility, and stress.

Results

The 44% of the interventions were designed by the research authors themselves, while the remaining 56% were replicated programs, which already had scientific evidence.

Discussion

Psychological interventions have a positive impact on sports performance. This review allows sports institutions and professionals to have more knowledge and resources at their disposal to implement these types of programs in their sports planning.
Research on the influence mechanism of employees’ innovation behavior in the context of digital transformation
Authors: Wu Jiale , Gong Xiheng , Liu Yijun
Published: December 2022

With the booming of digital economy, digital transformation has become an important initiative for the high-quality development of enterprises, however, in the process of digital transformation, the innovation performance of most enterprises will be reduced, and employee innovation is the basis of enterprise innovation. How to improve employee innovation ability and guarantee the continuous innovation of the enterprise becomes the key issue. By conducting expert interviews and data crawling, the study uses Grounded theory and Gioia method to sort out the influencing factors of employee innovation behavior in digital transformation enterprises and use the interpretative structural model to analyze the complex relationships among the influencing factors, and establish a hierarchical structural model to organize and hierarchize them. The study shows that: leadership style, organizational innovation level, and organizational social responsibility are the fundamental influencing factors, emotions and personality traits are the direct influencing factors, and innovation expectations and innovation support feedback are important undertaking factors. The above findings provide theoretical guidance for companies to motivate employees to innovate and improve their innovation performance in the process of digital transformation.
Evaluation of an assistance system supporting older pedestrians’ road crossing in virtual reality and in a real-world field test
Authors: Wiczorek Rebecca , Protzak Janna
Published: December 2022

Older pedestrians are at a high risk of becoming victims of car accidents because they tend not to pay sufficient attention to upcoming traffic. Within our research project, an assistance system for older pedestrians has been developed. It detects the street and communicates with the users through a vibrotactile interface. Two evaluation studies have been carried out in order to understand the potential benefits and drawbacks of the developed assistance system. One study was conducted in a virtual environment (VR) with 23 participants, aged 65+. The other experiment was a field test in a real street environment with 26 participants, aged 65+. Objective dependent variables in both experiments were checking for traffic (operationalized via head tracking) and stopping in front of the street (VR study), i.e., approaching time (field test). Workload and acceptance served as subjective dependent variables. Analysis of the VR experiment showed significantly more head rotation with the assistance system than without it, as well as significantly more with cars than without cars. The same was true for the frequency of stopping. No significant difference was found concerning workload. With regard to acceptance, the majority of participants indicated that the system was supportive and able to reduce risks in traffic. In the field test, results for head rotation confirmed the findings of the VR study. Analysis showed a marginally significant higher head rotation frequency with the alarm system than without, and significantly different patterns of checking for traffic at marked and unmarked crossings. However, unlike in the VR study, no differences were found in approaching time with and without the assistance system. Approaching time was slower at marked crossings. No difference was found with regard to workload, meaning the use of the assistance system did not increase the subjectively perceived workload of participants. Analysis of the acceptance questionnaire showed a positive attachment to the assistance system. However, most reported that they did not experience any advantage from the use of the system, and expressed no intention to buy such a system for themselves.
Medical students’ attitudes toward sleeping pill usage: A cross-sectional study
Authors: Alasmari Moudi M. , Alkanani Raghad S. , Alshareef Asmaa S. , Alsulmi Shahd S. , Althegfi Reuof I. , Bokhari Teef A. , Alsheikh Mona Y. , Alshaeri Heba K.
Published: December 2022

Medical students experience significant stress, which may interfere with their sleeping routines; consequently, they are at a higher risk of misusing sleeping pills. Therefore, this cross-sectional convenience sampling study aims to evaluate medical students’ attitudes toward the usage of sleeping pills, as well as the prevalence of their misuse and the associated factors. A self-administered online questionnaire survey was completed by 338 medical students at the College of Medicine of the King Saud bin Abdulaziz University for Health Sciences. Of 338 participants, 84 (24.85%) reported using sleeping pills. The prevalence of sleeping pill misuse was 26.63% (n = 90). The most common misuse behavior of sleeping pills was self-prescription (n = 72, 21.30%). The factors associated with sleeping pill misuse were stimulants usage (n = 69, 92%, p = 0.0072), high grade point average (n = 63, 84%, p = 0.046), preclinical years (n = 52, 69.3%, p = 0.042), and longer average time required to fall asleep (n = 53, 70.7%, p = 0.008). The prevalence of sleeping pill misuse is high among medical students, particularly among those in preclinical years. Therefore, enhancing awareness regarding sleeping pill misuse is crucial. This can be achieved through campaigns, workshops, and providing information regarding the dangers of sleeping pill misuse in the curriculum.
Quality of life in Vietnamese young adults: A validation analysis of the World Health Organization’s quality of life (WHOQOL-BREF) instrument
Authors: Vu Linh Gia , Nguyen Long Hoang , Nguyen Cuong Tat , Vu Giang Thu , Latkin Carl A. , Ho Roger C. M. , Ho Cyrus S. H.
Published: December 2022

Background

The abbreviated version of the World Health Organization’s Quality of Life (WHOQOL-BREF) instrument has been widely used to assess the quality of life (QOL) of different population groups.

Aims

This study aimed to examine the validity and reliability of the Vietnamese version of WHOQOL-BREF in evaluating the QOL of Vietnamese young adults.

Methods

The WHOQOL-BREF was validated in an online cross-sectional study among 445 young adults from 16 to 35 years in Vietnam. The exploratory factor analysis (EFA) and confirmatory factor analysis (CFA) were performed to examine the factorial structure of the instrument. The reliability and validity of the new factorial model were evaluated.

Results

The EFA and CFA suggested the 3-factor model had better fit models than the theoretical 4-factor model. The internal consistency of factor 1 “External life” and factor 2 “Internal life” were excellent (0.931) and good (0.864), respectively, while the internal consistency of factor 3 “Physical and mental health” was nearly acceptable (0.690). Results indicated that the 3-factor model had good convergent and divergent validity as well as moderate discriminant validity. Scores of factors “External life” and “Internal life” had significant predictive effects on general QOL, general health, and overall QOL (p< 0.05). Meanwhile, factor 3 “Physical and mental health” could only predict general health and overall QOL (p< 0.05).

Conclusion

This validation study improves understanding of the characteristics of QOL among young adults in Vietnam. While the theoretical model of WHO can be utilized for global comparisons, a new local model should be considered and cross-culturally adapted to successfully capture the progress of public health interventions for promoting young adults’ QOL.
Core self-evaluation and subjective wellbeing: A moderated mediation model
Authors: Chen Wei , Yang Tao , Luo Jing
Published: December 2022

Objectives

Much has been written documenting the positive association between core self-evaluation and adolescents' subjective wellbeing, but little is known about the mediating and moderating mechanisms which underlay this relationship. This study constructed a moderated mediation model to examine whether meaning in life mediated the relationship between core self-evaluation and subjective wellbeing, and whether this mediating process was moderated by adolescents' self-esteem.

Methods

A sample of 1,185 adolescents (11–17 years of age, 51.3% females) completed the Core Self-Evaluation Scale (CSES), the Meaning in Life Questionnaire (MLQ), the Rosenberg Self-esteem Scale (RSES), and the Index of Wellbeing Scale (IWS).

Results

The results indicate that after controlling for gender and age, core self-evaluation contributed significantly to subjective wellbeing (β = 0.900, p < 0.001). Meaning in life played a mediating role in the relationship between core self-evaluation and subjective wellbeing (β = 0.143, p < 0.01), with core self-evaluation indirectly affecting subjective wellbeing through meaning in life (βindirect = 0.068, 95% CI = [0.024, 0.119]). Self-esteem moderated the path mediated by meaning in life, more specifically, the conditional indirect effect between core self-evaluation and wellbeing was significant for adolescents with medium and low self-esteem (effect = 0.056, 95% CI = [0.014, 0.106]; effect = 0.092, 95% CI = [0.034, 0.159]. Both mediating and moderating effects were shown to exist in the association between core self-evaluation and adolescents' subjective wellbeing.

Discussion

Based on the results, the following suggestions can be made: subjective wellbeing can be promoted through interventions such as enhancing adolescents' core self-evaluation and helping them understand the meaning in life, and that greater attention needs to be paid to adolescents with low self-esteem. The findings of this study helpful to clarify the mediation and moderating mechanism of the beneficial influence of adolescents' core self-evaluation on subjective wellbeing.
Gender effects of project assessment: Evidence from a market simulation
Authors: Aquino Karl , Deretic Momo , Ries John
Published: December 2022

We investigate how males and females perform as entrepreneurs and traders using information on the trading activities of students participating in a business game in two university courses. In one course, students create entrepreneurial ventures that they pitch to their peers. These students are issued securities of all the ventures and trade in a simulated market based on information revealed in the classroom pitches. In the second course, students trade these ventures in a separate simulated market but do not see pitches and trade based on anonymized written information about the ventures. We measure student performance as entrepreneurs by the traded prices of their proposed ventures in the online market and performance as traders by the value of their closing portfolios. In the course where traders observe the sales pitches of the entrepreneurial teams, we find that both male and female traders buy and sell at lower prices when the female share of the venture team increases. Females buy at higher prices and sell at lower prices than males and end up with lower portfolio values than males. None of these results obtain in the course where trading is based on the same information delivered in written and anonymized form and the gender composition of the venture teams is not known. These findings provide insight on how the assessment and performance of tomorrow's business leaders is affected by environments involving direct sales pitches.