bluuebunny/crossref_metadata_2025 huggingface dataset:
Comprehensive Analysis of Cytochrome P450 Monooxygenases Reveals Insight Into Their Role in Partial Resistance Against Phytophthora sojae in Soybean
Authors: Khatri Praveen , Wally Owen , Rajcan Istvan , Dhaubhadel Sangeeta
Published: April 2022

Cytochrome P450 monooxygenases (P450) participate in the catalytic conversion of biological compounds in a plethora of metabolic pathways, such as the biosynthesis of alkaloids, terpenoids, phenylpropanoids, and hormones in plants. Plants utilize these metabolites for growth and defense against biotic and abiotic stress. In this study, we identified 346 P450 (GmP450) enzymes encoded by 317 genes in soybean where 26 GmP450 genes produced splice variants. The genome-wide comparison of both A-type and non-A-type GmP450s for their motifs composition, gene structure, tissue-specific expression, and their chromosomal distribution were determined. Even though conserved P450 signature motifs were found in all GmP450 families, larger variation within a specific motif was observed in the non-A-type GmP450s as compared with the A-type. Here, we report that the length of variable region between two conserved motifs is exact in the members of the same family in majority of the A-type GmP450. Analyses of the transcriptomic datasets from soybean-Phytophthora sojae interaction studies, quantitative trait loci (QTL) associated with P. sojae resistance, and co-expression analysis identified some GmP450s that may be, in part, play an important role in partial resistance against P. sojae. The findings of our CYPome study provides novel insights into the functions of GmP450s and their involvements in metabolic pathways in soybean. Further experiments will elucidate their roles in general and legume-specific function.
Transcriptome Profiling Reveals a Petunia Transcription Factor, PhCOL4, Contributing to Antiviral RNA Silencing
Authors: Xu Yingru , Ji Xiaotong , Xu Zhuangzhuang , Yuan Yanping , Chen Xiling , Kong Derong , Zhang Yanlong , Sun Daoyang
Published: April 2022

RNA silencing is a common antiviral mechanism in eukaryotic organisms. However, the transcriptional regulatory mechanism that controls the RNA silencing process remains elusive. Here, we performed high-depth transcriptome analysis on petunia (Petunia hybrida) leaves infected with tobacco rattle virus (TRV) strain PPK20. A total of 7,402 differentially expressed genes (DEGs) were identified. Of them, some RNA silencing-related transcripts, such asRNA-dependent RNA polymerases(RDRs),Dicer-like RNase III enzymes(DCLs), andArgonautes(AGOs), were induced by viral attack. Furthermore, we performed TRV-based virus-induced gene silencing (VIGS) assay on 39 DEGs encoding putative transcription factors (TFs), using green fluorescent protein (GFP) andphytoene desaturase(PhPDS) as reporters. Results showed that the down-regulation ofPhbHLH41,PhbHLH93,PhZPT4-3,PhCOL4,PhHSF-B3A,PhNAC90, andPhWRKY75led to enhanced TRV accumulation and inhibitedPhPDS-silenced photobleaching phenotype. In contrast, silencing ofPhERF22repressed virus accumulation and promoted photobleaching development. Thus, these TFs were identified as potential positive and negative regulators of antiviral RNA silencing, respectively. One positive regulatorPhCOL4, belonging to the B-box zinc finger family, was selected for further functional characterization. Silencing and transient overexpression ofPhCOL4resulted in decreased and increased expression of several RNA silencing-related genes. DNA affinity purification sequencing analysis revealed that PhCOL4 targetedPhRDR6andPhAGO4. Dual luciferase and yeast one-hybrid assays determined the binding of PhCOL4 to thePhRDR6andPhAGO4promoters. Our findings suggest that TRV-GFP-PhPDS-based VIGS could be helpful to identify transcriptional regulators of antiviral RNA silencing.
Psychosocial Stress, Epileptic-Like Symptoms and Psychotic Experiences
Authors: Bob Petr , Touskova Tereza Petraskova , Pec Ondrej , Raboch Jiri , Boutros Nash , Lysaker Paul
Published: April 2022

Background

Current research suggests that stressful life experiences and situations create a substantive effect in the development of the initial manifestations of psychotic disorders and may influence temporo-limbic epileptic-like activity manifesting as cognitive and affective seizure-like symptoms in non-epileptic conditions.

Methods

The current study assessed trauma history, hair cortisol levels, epileptic-like manifestations and other psychopathological symptoms in 56 drug naive adult young women experiencing their initial occurrence of psychosis.

Results

Hair cortisol levels among patients experiencing their initial episode of psychosis, were significantly correlated with stress symptoms measured by Trauma Symptom Checklist-40 (r = − 0.48, p < 0.01), and complex partial seizure-like symptoms measured by the Complex Partial Seizure-Like Symptoms Inventory (r = − 0.33, p < 0.05) and LSCL-33 (r = − 0.33, p < 0.05). Hair cortisol levels were not found to be significantly correlated with symptoms of anxiety and depression measured by Beck depression Inventory and Zung Anxiety Scale.

Conclusion

These findings suggest a significant relationship between epileptic-like symptoms and stress responses demonstrated by patients in their first psychotic episode. These findings may suggest the potential for research to explore usefulness of anticonvulsant treatment in patients who do not respond to usual psychotropic medication.
Predictive Processing in Sign Languages: A Systematic Review
Authors: Radošević Tomislav , Malaia Evie A. , Milković Marina
Published: April 2022

The objective of this article was to review existing research to assess the evidence for predictive processing (PP) in sign language, the conditions under which it occurs, and the effects of language mastery (sign language as a first language, sign language as a second language, bimodal bilingualism) on the neural bases of PP. This review followed the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) framework. We searched peer-reviewed electronic databases (SCOPUS, Web of Science, PubMed, ScienceDirect, and EBSCO host) and gray literature (dissertations in ProQuest). We also searched the reference lists of records selected for the review and forward citations to identify all relevant publications. We searched for records based on five criteria (original work, peer-reviewed, published in English, research topic related to PP or neural entrainment, and human sign language processing). To reduce the risk of bias, the remaining two authors with expertise in sign language processing and a variety of research methods reviewed the results. Disagreements were resolved through extensive discussion. In the final review, 7 records were included, of which 5 were published articles and 2 were dissertations. The reviewed records provide evidence for PP in signing populations, although the underlying mechanism in the visual modality is not clear. The reviewed studies addressed the motor simulation proposals, neural basis of PP, as well as the development of PP. All studies used dynamic sign stimuli. Most of the studies focused on semantic prediction. The question of the mechanism for the interaction between one’s sign language competence (L1 vs. L2 vs. bimodal bilingual) and PP in the manual-visual modality remains unclear, primarily due to the scarcity of participants with varying degrees of language dominance. There is a paucity of evidence for PP in sign languages, especially for frequency-based, phonetic (articulatory), and syntactic prediction. However, studies published to date indicate that Deaf native/native-like L1 signers predict linguistic information during sign language processing, suggesting that PP is an amodal property of language processing.

Systematic Review Registration

[https://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42021238911], identifier [CRD42021238911].
Associations Between Children’s Numeracy Competencies, Mothers’ and Fathers’ Mathematical Beliefs, and Numeracy Activities at Home
Authors: Mues Anna , Wirth Astrid , Birtwistle Efsun , Niklas Frank
Published: April 2022

Children’s numeracy competencies are not only relevant for their academic achievement, but also later in life. The development of early numeracy competencies is influenced by children’s learning environment. Here, the home numeracy environment (HNE) and parent’s own beliefs about mathematics play an important role for children’s numeracy competencies. However, only a few studies explicitly tested these associations separately for mothers and fathers. In our study, we assessed mothers’ and fathers’ mathematical gender stereotypes, self-efficacy and their beliefs on the importance of mathematical activities at home, and tested their associations with parents’ numeracy activities and children’s numeracy competencies in a sample of N = 160 children (n = 80 girls) with an average age of M = 59.15 months (SD = 4.05). Both, fathers and mothers regarded boys as being more competent in mathematics than girls. Fathers when compared to mothers reported a greater mathematical self-efficacy. Further, only mothers’ self-efficacy was associated with the frequency of numeracy activities with the study child. In contrast, only fathers’ beliefs on the importance of mathematics was associated with their numeracy activities which, in turn, predicted children’s numeracy competencies. However, the non-invariant constructs and varying results lead to the question whether a revision of existing scales assessing parental beliefs and home numeracy activities is needed to investigate differences of mothers and fathers and their potential associations with children’s numeracy outcomes.
Self-Rated Recovery and Mood Before and After Resistance Training and Muscle Microcurrent Application
Authors: Stößlein Bernd A. C. , Kuypers Kim P. C.
Published: April 2022

Background

Resistance training (RT) can offer beneficial physiological and psychological effects. The regular continuation of this exercise can be accomplished by improving the recovery and mood after a workout. Frequency-specific microcurrent (microstimulation) might offer a solution here as it has been shown to improve physical injuries, mood state, and sleep. However, knowledge is lacking about the impact of microstimulation after RT on said parameters. The present study aimed to test the effects of RT and muscle-microstimulation on mood and physical recovery in healthy men after performing conventional deadlifts, which is a type of RT.

Methods

The study was conducted according to a single-blind, randomized, placebo-controlled, and two-way crossover study. Twenty participants naïve to microstimulation (MS) engaged in RT twice on separate days. They were randomized to receive MS on 1 day and no microstimulation (Sham-MS) on another day. Before and after the workout and after their treatment (MS or Sham-MS), participants self-rated their mood state and mental and physical exhaustion levels.

Results

Findings showed that MS increased the self-ratings of well-rested and sociable and, most importantly, reduced the feeling of exercise-induced exhaustion. There were no MS effects on ratings of feeling sad, happy, or exhausted, although the workout, independent of MS, negatively influenced the level of exhaustion.

Conclusion

The combination of enhanced sociableness, reduced fatigue, and exercise-induced exhaustion after a workout, followed by microstimulation, has important implications for professional sporters and nonprofessionals who try to get the best result after a workout. Future studies using a double-blind approach including different types of exercises, different durations of programs, and both sexes can shed more light on the full potential of microstimulation after a workout on mood state and exercise-induced exhaustion.
Factorial Validity and Psychometric Properties of Exercise Dependence Scale Revised Among Lebanese Exercisers
Authors: Deflandre Anne , Kassabian Arine
Published: April 2022

The aim of this manuscript is to discuss the reliability and the factorial, convergent and discriminant validities in measurement model of the 21-item Exercise Dependence Scale Revised (EDS-R) used for diagnosing exercise addiction. A study was conducted among 695 (364 male and 331 female) Lebanese bilingual young adults (aged 18–25) who filled the English version of this questionnaire, along with a general questionnaire. This study showed that, despite an acceptable fit index confirming the 7-factor structure and invariance across gender, exercise duration and age groups; the EDS-R lacks in reliability and convergent and discriminant validities in measurement model, due particularly to the “Reduction in other activities” subscale. The construction of the EDS-R is then to be discussed.
Detecting Suicide Ideation in the Era of Social Media: The Population Neuroscience Perspective
Authors: Morese Rosalba , Gruebner Oliver , Sykora Martin , Elayan Suzanne , Fadda Marta , Albanese Emiliano
Published: April 2022

Social media platforms are increasingly used across many population groups not only to communicate and consume information, but also to express symptoms of psychological distress and suicidal thoughts. The detection of suicidal ideation (SI) can contribute to suicide prevention. Twitter data suggesting SI have been associated with negative emotions (e.g., shame, sadness) and a number of geographical and ecological variables (e.g., geographic location, environmental stress). Other important research contributions on SI come from studies in neuroscience. To date, very few research studies have been conducted that combine different disciplines (epidemiology, health geography, neurosciences, psychology, and social media big data science), to build innovative research directions on this topic. This article aims to offer a new interdisciplinary perspective, that is, a Population Neuroscience perspective on SI in order to highlight new ways in which multiple scientific fields interact to successfully investigate emotions and stress in social media to detect SI in the population. We argue that a Population Neuroscience perspective may help to better understand the mechanisms underpinning SI and to promote more effective strategies to prevent suicide timely and at scale.
Do Predictors of Health Facility Delivery Among Reproductive-Age Women Differ by Health Insurance Enrollment? A Multi-Level Analysis of Nigeria's Data
Authors: Zhang Xiaomei , Anser Muhammad Khalid , Ahuru Rolle Remi , Zhang Zizai , Peng Michael Yao-Ping , Osabohien Romanus , Mirza Mumal
Published: April 2022

This study aims to compare determinants of health facility delivery for women under a health insurance scheme and those not under a health insurance scheme. Secondary data drawn from the National Demographic and Health Survey was used for the analysis. The characteristics of the women were presented with simple proportions. Binary multilevel logistic regression was used to examine the determinants of health facilities for women who enrolled in health insurance and those who did not. All statistical analyses were set at 5% level of significant level (p= 0.24). The result showed that 2.1% of the women were under a health insurance scheme. Disparity exists in health insurance ownership as a higher proportion of those enrolled in health insurance were those with higher education attainment, in urban parts of the country, and those situated on higher wealth quintiles. There is a significant difference between those with and those without health insurance. It implies that a higher proportion of women who enrolled in health insurance delivered in health facility delivery compared to those who do not. The unique determinants of health facility delivery for women under health insurance were parity and birth order, while unique determinants of health facility delivery for women not enrolled in health schemes were employment status, marriage type, and geopolitical zones. Uniform predictors of health facility delivery for both groups of women were maternal education, household wealth quintiles, autonomy on healthcare, number of antenatal contacts, residential status, community-level poverty, community-level media use, and community-level literacy. Intervention programs designed to improve health facility delivery should expand educational opportunities for women, improve household socioeconomic conditions, target rural women, and encourage women to undertake a minimum of four antenatal contacts.
Prevalence of Physical Activity and Sedentary Behavior Patterns in Generally Healthy European Adults Aged 70 Years and Older—Baseline Results From the DO-HEALTH Clinical Trial
Authors: Mattle Michèle , Meyer Ursina , Lang Wei , Mantegazza Noemi , Gagesch Michael , Mansky Richard , Kressig Reto W. , Egli Andreas , Orav E. John , Bischoff-Ferrari Heike A.
Published: April 2022

Background

Physical activity (PA) is important for healthy aging and disease prevention whereas sedentary behavior (SB) accelerates health deterioration.

Aim

To investigate activity profiles regarding PA and SB among generally healthy European older adults.

Methods

Meeting PA recommendations was defined as ≥150 min/week of moderate and/or ≥75 min/week of vigorous PA. A cut-off of ≥5.5 h/day was used to define time spent with SB. We present prevalence of PA and SB overall and by sex, age, BMI, and country. We examined correlates with multivariate logistic regression models.

Results

Two thousand one hundred and fifty-five DO-HEALTH participants completed baseline information on activity profiles [mean age 74.9 years (SD 4.5), 61.8% women]. Overall, 62.2% met PA recommendations and overall, 37.1% spent ≥5.5 h/day with SB. Younger participants (70–74 years), men, and those with BMI <25 kg/m2 met PA recommendations more often. Per country, prevalence of meeting PA recommendations were: Austria 74.4%, France 51.0%, Germany 65.6%, Portugal 46.5%, and Switzerland 66.7%. Regarding SB, prevalence did not differ in all subgroups. In multivariate logistic regression analyses, being male, younger age, lower MoCA scores, and higher SPPB score were associated with greater odds, whereas higher BMI, more years of education, higher GDS score, and residing in Portugal were associated with lower odds of meeting PA recommendations. High BMI and higher MoCA scores were associated with greater odds of high SB.

Conclusion

Individualized public health efforts may be warranted even in active older adults, as profiles were less favorable in subgroups of older age, female sex and higher BMI.
Metaphors of War in Effective and Ineffective Coping of Medical Directors of COVID-19 Wards in Public Hospitals
Authors: Naamati-Schneider Lior , Gabay Gillie
Published: April 2022

The COVID-19 pandemic has challenged medical professionals worldwide with an unprecedented need to provide care under conditions of complexity, uncertainty, and danger. These conditions, coupled with the unrelenting stress of overwhelming workloads, exhaustion, and decision-making fatigue, have forced clinicians to generate coping mechanisms. This qualitative study explored the use of metaphors as a coping mechanism by clinical directors of COVID-19 wards in Israeli public general hospitals while they were exposed to death and trauma throughout the pandemic's first wave in Israel (March to June 2020). The study employs discourse methodology and metaphor mapping analysis to capture the personal, organizational, and social dimensions of effective and ineffective processes of coping with an extreme health crisis. Analysis revealed that the metaphors that clinical directors used reflect a dual process of mediating and generating the social construction of meaning and facilitating effective and ineffective coping. Effective coping was facilitated by war metaphors that created a sense of mission and meaningfulness at both the organizational and the individual levels. War metaphors that generated a sense of isolation and sacrifice intensified helplessness and fear, which undermined coping. We propose actionable recommendations to enhance effective coping for individuals and organizations in this ongoing pandemic.
Examining the Interplay of Smartphone Use Disorder, Mental Health, and Physical Symptoms
Authors: Reer Felix , Wehden Lars-Ole , Janzik Robin , Quandt Thorsten
Published: April 2022

The current study examined antecedents and possible consequences of smartphone use disorder (SmUD). In particular, we aimed to increase the understanding of the interplay of SmUD, mental health, and physical symptoms. Studies found that SmUD is associated with diverse psychological and physical health impairments, ranging from depression and anxiety to headaches and sleep disturbances. Based on existing works, we assumed that mental problems mediate the relationship between SmUD and bodily problems. We conducted a cross-sectional random-quota online survey among 938 German smartphone owners aged 14 to 64 years. An instrument based on the 5th edition of the Diagnostic and Statistical Manual of Mental Disorders (DSM-5) was used to measure SmUD severity. The data was analyzed using structural equation modeling. We identified a rate of 4.0% potentially disordered users. Males and younger participants showed more signs of SmUD. As expected, SmUD severity was found to be associated with physical (more frequent headaches, sleep disturbances, gastrointestinal problems) as well as psychological (higher levels of loneliness, stress, depression/anxiety) health impairments. Investigating the interplay of these variables showed that depression and anxiety, and stress partially mediated the relationship between SmUD severity and physical symptoms. Taken together, our results confirm that increased SmUD severity is associated with mental problems as well as with somatic symptoms. We assume complex (and presumably circular) relationships, which future studies should examine in more detail. SmUD prevention and intervention programs should follow a broad approach that considers decreases in physical and mental health, possibly causing or resulting from SmUD.
Hands to Hexapods, Wearable User Interface Design for Specifying Leg Placement for Legged Robots
Authors: Zhou Jianfeng , Nguyen Quan , Kamath Sanjana , Hacohen Yaneev , Zhu Chunchu , Fu Michael J. , Daltorio Kathryn A.
Published: April 2022

Specifying leg placement is a key element for legged robot control, however current methods for specifying individual leg motions with human-robot interfaces require mental concentration and the use of both arm muscles. In this paper, a new control interface is discussed to specify leg placement for hexapod robot by using finger motions. Two mapping methods are proposed and tested with lab staff, Joint Angle Mapping (JAM) and Tip Position Mapping (TPM). The TPM method was shown to be more efficient. Then a manual controlled gait based on TPM is compared with fixed gait and camera-based autonomous gait in a Webots simulation to test the obstacle avoidance performance on 2D terrain. Number of Contacts (NOC) for each gait are recorded during the tests. The results show that both the camera-based autonomous gait and the TPM are effective methods in adjusting step size to avoid obstacles. In high obstacle density environments, TPM reduces the number of contacts to 25% of the fixed gaits, which is even better than some of the autonomous gaits with longer step size. This shows that TPM has potential in environments and situations where autonomous footfall planning fails or is unavailable. In future work, this approach can be improved by combining with haptic feedback, additional degrees of freedom and artificial intelligence.
Biomechanics of World-Class 800 m Women at the 2017 IAAF World Championships
Authors: Hanley Brian , Merlino Stéphane , Bissas Athanassios
Published: April 2022

The 800 m race challenges the aerobic and anaerobic energy systems, and athletes adopt a technique that allows for running efficiency as well as sprinting speeds. The aim of this novel study was to compare important kinematic variables between the two laps of the 2017 IAAF World Championships women's final. Video data (150 Hz) were collected of all eight finalists on both laps at a distance approximately 50 m from the finish line along the home straight. Running speed, step length, cadence, temporal variables, sagittal plane joint angles, and shank angle at initial contact were measured. Running speed was faster on lap 2 (p= 0.008) because of large increases in cadence (p= 0.012). These higher cadences resulted in large decreases in contact times (p= 0.031) and in flight times (p= 0.016) on lap 2. Greater knee flexion and ankle plantarflexion (p≤ 0.039) at initial contact on lap 2 seemed partly responsible for shorter swing times (p= 0.016), and which accompanied a decrease in shank angle at initial contact from lap 1 (7°) to a more vertical position on lap 2 (4°) (p= 0.008). Coaches should note that the need for higher cadence, horizontal impulse production during shorter contact times, and the adoption of forefoot striking require strength and neural system training to allow for athletes to increase cadence during the sprint finish. Increasing cadence (and not step length) was the driving factor for faster finishing speeds in the women's 800 m.
The Impact of Frame Running on Quality of Life in Young Athletes With Mobility Limitations
Authors: van Schie Petra E. M. , van Eck Mirjam , Bonouvrié Laura A. , Edelman Bos Arnoud M. M. , Buizer Annemieke I.
Published: April 2022

Purpose

The para-athletic sport Frame Running is developed for persons with neurological impairments causing severe limitations of walking ability. Participating in sports can contribute to a better quality of life (QoL). It is unknown if participation in Frame Running contributes to QoL in children with mobility limitations. This study aims to explore the changes in QoL in children and youth who started Frame Running.

Materials and Methods

We conducted a cross-sectional study amongst young Frame Running athletes with mobility limitations due to various underlying causes, aged 6–19 years, who are members of one of the Frame Running groups in the Netherlands. For 62 athletes, parents completed the Psychosocial Impact of Assistive Devices Scale (PIADS) questionnaire (subscales: competence, adaptability, and self-esteem). For six athletes, parents were interviewed to obtain more in-depth insight in the perceived changes in the QoL of their children.

Results

Parents (of 58% boys, mean age 12 years 4 months; SD 3 years 3 months; 52% supported walkers) reported a significant positive change on all three subscales of the PIADS questionnaire since their children started Frame Running. Most change was experienced in the items performance, the ability to participate, happiness and self-confidence. Quotes of the parents who were interviewed elucidated these changes.

Conclusion

Frame Running increased QoL in young athletes with a mobility limitation. Frame Running may therefore be advised for these children and youth to improve QoL.
Role of Wild Food Environments for Cultural Identity, Food Security, and Dietary Quality in a Rural American State
Authors: Ahmed Selena , Warne Teresa , Stewart Alyssa , Byker Shanks Carmen , Dupuis Virgil
Published: April 2022

Wild foods are primary components of traditional and Indigenous food systems that are valued for food security while being vulnerable to global change. This case study examines practices, experiences, and perceptions associated with wild food environments through a household survey in the rural American state of Montana. Findings highlight that wild food environments contribute to cultural identity, sense of place, food security, and dietary quality of surveyed households while being vulnerable to loss of traditional ecological knowledge as well as climate and land-use change. Of the 182 informants, 80% hunt, 83% fish, and 68% forage wild botanicals. More than half of the informants agreed that wild food procurement is part of their cultural identity (66%). Collectively, informants procure more than 172 wild food species with the most prevalent being deer, waterfowl, elk, trout, bass, a range of berries, mushrooms, and botanicals used medicinally. Participants have a multidimensional value system where wild food procurement is valued for diets, recreation, family time, spirituality, and connection to the environment. The majority of participants agreed that the consumption of wild foods contributes to the nutritional quality (87%) and diversity (82%) of their diets while lowering food costs (59%). At least half of the informants reported observing changes in climate patterns over the past decade including increased temperature (50%) and more extreme and variable weather patterns (38%) that they perceive are impacting wild food environments including shifts in wild game, fish, and edible plant populations. Based on findings, we support that wild food environments and associated bio-cultural resources are a critical place to understand, conserve, and promote for nutrition. We thus advance the concept of “conservation for nutrition”. Community engagement, education, and policy plans are called for to promote wild food environments toward supporting sustainable diets and planetary health.
Correlation Between Post-operative Sense of Coherence and Family Function in Patients With Type A Aortic Dissection
Authors: Zhang Xiaomin , Chen Juan , Zang Yaning
Published: April 2022

Objective

To analyze the relationship between post-operative sense of coherence and family function in patients with type A aortic dissection (AD).

Methods

Ninety patients with AD treated from January 2019 to December 2020 were selected as the research subjects. All patients received surgical treatments. Two weeks after the operation, the Sense of Coherence Scale (SOC-13) and Family APGAR index scale (APGAR) were used to evaluate the patients' sense of coherence and family function. Baseline data of all patients were collected, the SOC-13 scores of patients with type A AD with different demographic characteristics were compared, and the relationship between family function and patients' sense of coherence was analyzed.

Results

The ninety patients with type A AD had a low level of psychological consistency, and the average SOC-13 score was 49.84 ± 3.89 points. The SOC-13 score of patients with type A AD with family monthly incomes <5,000 yuan and moderate and severe family dysfunction was lower than that of patients with family monthly incomes ≥5,000 yuan and good family function. The difference was statistically significant (P < 0.05). There was no statistically significant difference in the SOC-13 scores of patients with type A AD with different demographic characteristics (P > 0.05). The results of multiple linear regression analysis showed that family monthly income <5,000 yuan and moderate and severe family dysfunction might be general influencing factors of sense of coherence among patients with type A AD (P < 0.05). Y = 43.333 + 6.667X1 + 16.730X2 was obtained.

Conclusion

The post-operative sense of coherence of patients with type A AD may be affected by family function.
Whole Transcriptome Profiling of the Effects of Cadmium on the Liver of the Xiangxi Yellow Heifer
Authors: Wei Yameng , Yi Kangle , Shen Caomeihui , Chen Xue , Iqbal Tariq , Cao Maosheng , Chen Tong , Luo Yang , Li Jianbo , Zhou Xu , Li Chunjin , Chen Lu
Published: April 2022

Cadmium (Cd) is a major heavy metal toxicant found in industrial zones. Humans and animals are exposed to it through their diet, which results in various physiological problems. In the current study, the toxic effects of Cd on the liver were investigated by whole-transcriptome sequencing (RNA-seq) of the livers of Xiangxi heifers fed a diet with excess Cd. We randomly divided six healthy heifers into two groups. The first group received a control diet, whereas the second group received Cd-exceeding diets for 100 days. After 100 days, the livers were collected. A total of 551 differentially expressed mRNAs, 24 differentially expressed miRNAs, and 169 differentially expressed lncRNAs were identified (p < 0.05, |log2FC| >1). Differentially expressed genes (DEGs) were analyzed by gene ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses. We found that under Cd exposure, DEGs were enriched in the adenosine 5'-monophosphate–activated protein kinase pathway, which is involved in autophagy regulation, and the peroxisome proliferator–activated receptor pathway, which is involved in lipid metabolism. In addition, the apolipoprotein A4 gene, which has anti-inflammatory and antioxidant effects, the anti-apoptotic gene ATPase H+/K+ transporting the nongastric alpha2 subunit, and the cholesterol metabolism–associated gene endothelial lipase gene were significantly downregulated. C–X–C motif chemokine ligand 3, cholesterol 7α-hydroxylase, and stearoyl-CoA desaturase, which are involved in the development of fatty liver, were significantly upregulated. These genes revealed the main effects of Cd on the liver of Xiangxi yellow heifers. The current study provides insightful information regarding the DEGs involved in autophagy regulation, apoptosis, lipid metabolism, anti-inflammation, and antioxidant enzyme activity. These may serve as useful biomarkers for predicting and treating Cd-related diseases in the future.
Milk Immune Cell Composition in Dromedary Camels With Subclinical Mastitis
Authors: Alhafiz Gader Abdulaziz , Alghatam Fatema Hassan , Almohammed Hams , Hussen Jamal
Published: April 2022

Mastitis represents one of the most important infectious diseases in camels with heavy economic losses due to reduced milk quantity and quality. Balanced immune cell composition and function in the mammary gland are essential for effective immune response to mastitis pathogens. The objective of the present study was to characterize the cellular immune response to subclinical mastitis in the mammary gland of dromedary camels. Therefore, immunostaining and flow cytometry were used to compare the cellular composition, leukocyte phenotype, and cell viability in camel milk from healthy she-camels (n= 8) and she-camels with subclinical mastitis (SCM;n= 6). In addition, theex vivophagocytic activity of milk phagocytes was compared between healthy and affected animals. The health status of the mammary gland was evaluated based on the California Mastitis Test (CMT) score. SCM (CMT score of ≥3 in the absence of clinical signs of mastitis) was found in six of the 56 sampled quarters (10.7 %) with only one affected quarter per animal. In comparison to milk from healthy camels, milk from SCM animals showed higher somatic cell count (SCC), higher numbers of CD45+ leukocytes with an expanded fraction of CD172a+ myeloid cells. Within the myeloid cell population, there was an increase in the percentage of granulocytes (CD172a+CD14low) with a decreased percentage of macrophages (CD172a+CD14high) in milk from affected animals compared to healthy animals. The decrease in lymphoid cells in SCM milk was mainly due to the decreased fraction of CD4+ helper T cells. Camel SCM was also associated with a stimulated phenotype, increased cell viability, and enhanced phagocytic activity of the milk phagocytes, macrophages and granulocytes. Collectively, the present study identified significant changes in SCC, leukocyte count, phenotype, viability, and function in association with subclinical mastitis in camels. The results of the present study support a better understanding of host-pathogen interaction mechanisms in the camel mammary gland.
The New Terrorism Revisited: Some Remarks on Terrorism Evolution and Its Strategic Significance After 9/11
Authors: Madej Marek
Published: April 2022

The article discusses main evolutionary trends in terrorism after 9/11. Analysing the phenomenon globally and with reference to the concept of so called “new terrorism”, the articles points out the continuation of majority of trends in terrorism’ evolution from the past, particularly on motivational and organizational level. The study stresses, however, significant quantitative growth in terrorist acts and changes in their geographical distributions. In addition, the article analyses reasons why the risks of super- and cyberterrorism did not materialized fully in last decades.
Biometric Technology 20 Years After 9/11 – Opportunities and Threats
Authors: Tomaszewska-Michalak Magdalena
Published: April 2022

The aim of the article is to present the development of biometric technology as a consequence of 9/11 terrorist attacks in the USA and issuing the PATRIOT ACT in 2001. Nowadays biometric technology is popular not only in the public security area (e.g. criminal data bases, face recognition surveillance systems) but is also used in everyday life (e.g. smartphones with touch ID/face recognition). The article shows both sides of biometric devices utilization: advantages and potential negative consequences for individuals.
A Statistical Approach to Discovering Process Regime Shifts and Their Determinants
Authors: Siddiqui Atiq W. , Raza Syed Arshad
Published: April 2022

Systematic behavioral regime shifts inevitably emerge in real-world processes in response to various determinants, thus resulting in temporally dynamic responses. These determinants can be technical, such as process handling, design, or policy elements; or environmental, socio-economic or socio-technical in nature. This work proposes a novel two-stage methodology in which the first stage involves statistically identifying and dating all regime shifts in the time series process event logs. The second stage entails identifying contender determinants, which are statistically and temporally evaluated for their role in forming new behavioral regimes. The methodology is general, allowing varying process evaluation bases while putting minimal restrictions on process output data distribution. We demonstrated the efficacy of our approach via three cases of technical, socio-economic and socio-technical nature. The results show the presence of regime shifts in the output logs of these cases. Various determinants were identified and analyzed for their role in their formation. We found that some of the determinants indeed caused specific regime shifts, whereas others had no impact on their formation.
Design and Performance of X-Band SAR Payload for 80 kg Class Flat-Panel-Type Microsatellite Based on Active Phased Array Antenna
Authors: Kim Seok , Song Chan-Mi , Lee Seung-Hun , Song Sung-Chan , Oh Hyun-Ung
Published: April 2022

The small synthetic aperture radar (SAR) technology experimental project (S-STEP) mission aims to develop an innovative spaceborne SAR microsatellite as a constellation of 32 microsatellites featuring a high-resolution stripmap mode of 1 m. The S-STEP is a spaceborne SAR microsatellite technology demonstration program in which innovative approaches have been proposed and investigated for SAR payload system designs for improving the development speed, affordability, size and weight parameters, and quality of SAR satellite systems. In this study, the major design approach includes a bus–payload integrated flat-panel-type SAR payload based on an active phased-array antenna. This study conducted an SAR image performance analysis considering the mission requirements to validate the feasibility of the innovative SAR payload design of the S-STEP. These performance analysis results are presented to demonstrate the effectiveness of the proposed SAR payload design approach under the new space paradigm.
Design and Experiment of an Integrated Automatic Transplanting Mechanism for Picking and Planting Pepper Hole Tray Seedlings
Authors: Hu Shuangyan , Hu Minjuan , Yan Wei , Zhang Wenyi
Published: April 2022

The operation of the semi-automatic pepper pot seedling transplanter has the problem of a low frequency of manual operation. We designed a new automatic transplanting mechanism based on a clamping stem. Through the movement law of a double-crank connecting rod mechanism, the static trajectory of beak shape and the dynamic trajectory of N shape were designed to meet the requirements of backward transplanting. The kinematics equation of the seedling picking mechanism was established and the mechanism parameter optimization program was developed based on MATLAB. The effect of the mechanism parameter changes on the seedling movement trajectory was analyzed. A group of parameter combinations that met the requirements of seedling picking and planting were optimized. After optimization, the trajectory height was 220.8 mm, the picking angle was 21.03° and the planting angle was 64.15°. The plant spacing was 260 mm, which meets the agronomic requirements of pepper transplanting. Through the combination of theoretical analysis and prototype tests, the test results showed that the theoretical trajectory was basically consistent with the actual trajectory, verifying the feasibility of the mechanism design. In this study, pepper pot seedlings in the suitable planting period of 60 days were selected and planted at the rotation speed of 60, 70 and 80 r/min, respectively. The success rate of seedling selection was more than 91.1%, the success rate of planting was more than 78.5%, the qualified rate of erect degree was 94.9%, and the coefficient of variation of plant spacing was stable below 14.1%. The results show that the integrated automatic transplanting mechanism for pepper pot seedlings was effective.
Potential Environmental Impacts of Peanut Using Water Footprint Assessment: A Case Study in Georgia
Authors: Deepa Raveendranpillai , Anandhi Aavudai , Bailey Nathaniel O. , Grace Johnny M. , Betiku Omolola C. , Muchovej James J.
Published: April 2022

The recent decade has witnessed an increase in irrigated acreage in the southeast United States due to the shift in cropping patterns, climatic conditions, and water availability. Peanut, a major legume crop cultivated in Georgia, Southeast United States, has been a staple food in the American household. Regardless of its significant contribution to the global production of peanuts (fourth largest), studies related to local or regional scale water consumption in peanut production and its significant environmental impacts are scarce. Therefore, the present research contributes to the water footprint of peanut crops in eight counties of Georgia and its potential ecological impacts. The impact categories relative to water consumption (water depletion—green and blue water scarcity) and pesticide use (water degradation—potential freshwater ecotoxicity) using crop-specific characterization factors are estimated for the period 2007 to 2017 at the mid-point level. These impacts are transformed into damages to the area of protection in terms of ecosystem quality at the end-point level. This is the first county-wise quantification of the water footprint and its impact assessment using ISO 14046 framework in the southeast United States. The results suggest inter-county differences in water consumption of crops with higher blue water requirements than green and grey water. According to the water footprint analysis of the peanut crop conducted in this study, additional irrigation is recommended in eight Georgia counties. The mid-point level impact assessment owing to water consumption and pesticide application reveals that the potential freshwater ecotoxicity impacts at the planting and growing stages are higher for chemicals with high characterization factors regardless of lower pesticide application rates. Multiple regression analysis indicates blue water, yield, precipitation, maximum surface temperature, and growing degree days are the potential factors influencing freshwater ecotoxicity impacts. Accordingly, a possible impact pathway of freshwater ecotoxicity connecting the inventory flows and the ecosystem quality is defined. This analysis is helpful in the comparative environmental impact assessments for other major crops in Georgia and aids in water resource management decisions. The results from the study could be of great relevance to the southeast United States, as well as other regions with similar climatic zones and land use patterns. The assessment of water use impacts relative to resource availability can assist farmers in determining the timing and layout of crop planting.
Effect of β-Glucan Supplementation on Growth Performance and Intestinal Epithelium Functions in Weaned Pigs Challenged by Enterotoxigenic Escherichia coli
Authors: Zhou Yuankang , Luo Yuheng , Yu Bing , Zheng Ping , Yu Jie , Huang Zhiqing , Mao Xiangbing , Luo Junqiu , Yan Hui , He Jun
Published: April 2022

Background: To examine the effect of β-glucan (BGL) supplementation on growth performance and intestinal epithelium functions in weaned pigs upon Enterotoxigenic Escherichia coli (ETEC) challenge. Methods: Thirty-two weaned pigs (Duroc × Landrace × Yorkshire) were assigned into four groups. Pigs fed with a basal diet or basal diet containing 500 mg/kg BGL were orally infused with ETEC or culture medium. Results: Results showed BGL tended to increase the average daily gain (ADG) in ETEC-challenged pigs (0.05 < p < 0.1). Dietary BGL supplementation had no significant influence on nutrient digestibility (p > 0.05). However, BGL improved the serum concentrations of immunoglobulin (Ig) A and IgG, and was beneficial to relieve the increasement of the concentrations of inflammatory cytokines such as the TNF-α and IL-6 upon ETEC-challenge (p < 0.05). Interestingly, BGL significantly increased the duodenal, jejunal and ileal villus height, and increased the jejunal ratio of villus height to crypt depth (V/C) upon ETEC challenge (p < 0.05). BGL also increased the activities of mucosal, sucrase and maltase in the ETEC-challenged pigs (p < 0.05). Moreover, BGL elevated the abundance of Lactobacillus and the concentration of propanoic acid in colon in the ETEC-challenged pigs (p < 0.05). Importantly, BGL elevated the expression levels of zonula occludins-1 (ZO-1) and mucin-2 (MUC-2) in the small intestinal mucosa upon ETEC challenge (p < 0.05). BGL also upregulated the expressions of functional genes such as the claudin-1, cationic amino acid transporter-1 (CAT-1), LAT-1, L amino acid transporter-1 (LAT1), fatty acid transport proteins (FATP1), FATP4, and sodium/glucose cotransporter-1 (SGLT-1) in the duodenum, and the occludin-1 and CAT-1 in the jejunum upon ETEC challenge (p < 0.05). Conclusions: These results suggested that BGL can attenuate intestinal damage in weaned pigs upon ETEC challenge, which was connected with the suppressed secretion of inflammatory cytokines and enhanced serum immunoglobulins, as well as improved intestinal epithelium functions and microbiota.
Hop Tannins as Multifunctional Tyrosinase Inhibitor: Structure Characterization, Inhibition Activity, and Mechanism
Authors: Liu Jiaman , Chen Yanbiao , Zhang Xinxin , Zheng Jie , Hu Weiying , Teng Bo
Published: April 2022

The application of hops could be extended to obtain higher commercial values. Tannins from hops were assessed for their tyrosinase inhibition ability, and the associated mechanisms were explored. Nuclear magnetic resonance (NMR) and high-performance liquid chromatography–electrospray ionization–tandem mass spectrometry (HPLC–ESI–MS/MS) revealed that the hop tannins were characterized as condensed tannins with (epi)catechin and (epi)gallocatechin as subunits and an average polymerization degree of 10.32. Tyrosinase inhibition assay indicated that hop tannins had an IC50 = 76.52 ± 6.56 μM. Kinetic studies of the inhibition processes indicated the tannins provided inhibition through competitive–uncompetitive mixed reactions. In silico molecule docking showed that tannins were bound to the active site of tyrosinase via hydrogen and electrovalent bonds. Circular dichroism (CD) observed the structural variation in the tyrosinase after reacting with the tannins. Fluorescence quenching analysis and free radical scavenging assays indicated that the tannins had copper ion chelating and antioxidant activities, which may also contribute to inhibition. The intracellular inhibition assay revealed that the melanin was reduced by 34.50% in B16F10 cells. These results indicate that these tannins can be applied as whitening agents in the cosmetics industry.