bluuebunny/crossref_metadata_2025 huggingface dataset:
Uneven Stiffness Coal Seam: A New Structural Factor Prone to Coal Burst Based on Stiffness Theory
Authors: Tang Tiewu , Deng Zhigang , Ren Weiguang , Zhao Shankun , Wang Yin , Zhao Yang , Li Wenxin
Published: December 2023

The development of stiffness theory is constrained by its contradiction with engineering experience. Several easily overlooked details of stiffness theory were clarified, and a qualitative evaluation formula for the risk of coal burst was provided. Then a novel structure factor called uneven stiffness coal seam structure (USCS), which consists of high stiffness zone (HSZ), low stiffness zone (LSZ), and contiguous roof and floor, was proposed. Many areas prone to coal bursts, such as thinning zones, bifurcating areas, magmatic intrusion areas, and remnant pillar affected areas of coal seam, are the HSZs of USCSs. Comparative analysis of the uneven stiffness coal seam under different roof conditions and examination of the simplified trisection model of the USCS were conducted. Then 6 groups of 14 simplified 2D models using COMSOL5.2 was constructed based on controlled variable method to simulate different responses of the USCS with varying parameters under same working conditions. The results demonstrate the following: (1) coal bursts occur only when both the failure criterion and the stiffness criterion are simultaneously satisfied, the risk of coal burst (rCB) is the product of the risk of failure (rF) and the risk of instability (rI). (2) The pressure concentration function of USCS facilitates stress concentration from LSZ to HSZ, thus raising the rF in HSZ. The stiffness reduction function of USCS reduces the local mine stiffness (LMS) of the HSZ, allowing the system to meet the stiffness criterion even with a hard roof, thereby raising the rI in HSZ and reconciling the contrast between stiffness theory and engineering experience. Failures within HSZ of the USCS enables the roof strata to release bending deformation energy without roof breakage. (3) The normal stress of HSZ is positively correlates with the value of ERHRKHSL/KLSH; The LMS of the HSZ is positively correlated with the value of ERKL/KHHRSLSH. The USCS boasts significant advantages in integrating and harmonizing various existing theories and explaining multiple specific types of coal bursts. By applying relevant USCS findings, new explanations can be provided for engineering phenomena such as the time-delayed coal bursts, the inefficient pressure relief in ultra thick coal seams, and the “microseism deficiency” observed prior to certain coal bursts.
Advances in Pretreatment Methods for Free Nucleic Acid Removal in Wastewater Samples: Enhancing Accuracy in Pathogenic Detection and Future Directions
Authors: Vu Kien A. , Nguyen Thu A. , Nguyen Thao P.
Published: December 2023

Accurate pathogenic detection in wastewater is critical for safeguarding public health and the environment. However, the presence of free nucleic acids in wastewater samples poses significant challenges to molecular detection accuracy. This comprehensive review explores the current status and future potential of pretreatment methods to remove free nucleic acids from wastewater samples. The study contributes a comprehensive analysis of the mechanisms, strengths, and limitations of various pretreatment approaches, including physical, chemical, and enzymatic processes. The effect of various factors on the removal efficiency of these pretreatment methods is also discussed. This review enhances our comprehension of pretreatment techniques and their vital role in achieving precise pathogenic detection in complex wastewater matrices. Furthermore, it outlines future perspectives and developments for improving the speed and effectiveness of pathogenic detection, contributing significantly to disease surveillance, early warning systems, and environmental protection.
The Effect of Leader–Follower Psychological Capital Congruence on Safety Behavior
Authors: Wang Dan , Qin Yunyun , He Yang
Published: December 2023

This study aims to explore the relationship between leader and follower psychological capital (PC) congruence, intrinsic safety motivation (ISM), and safety behavior (SB). The construction industry is one of the most dangerous industries, and most safety accidents are determined by human behavior. Exploring the factors influencing construction workers’ SB is important for improving construction safety. This study collected 242 paired questionnaires from frontline construction workers and crew leaders in China. The SPSS 22.0 software and PROCESS program were used for analysis, the empirical study was conducted using polynomial regression and response surface analysis, and the block variable method was used to test for mediating effects. The findings show that (a) the level of follower SB is determined by the degree of leader–follower PC congruence; (b) when leader–follower PC was congruent, the impact of both high PCs on follower SB was enhanced compared to each low PC; (c) when leader–follower PC was incongruent, the impact of follower PC (FPC) above leader PC (LPC) was better on follower SB compared to FPC below LPC; (d) the relationship between leader–follower PC congruence and follower SB was mediated by ISM. The promotion of SB should focus on the fit between leaders and followers in addition to the PC of the followers themselves. This study contributes to workgroup safety in the construction industry by improving the fit between workgroup leaders and members, promoting ISM, and thus improving their safety performance.
Study on the Stability of Accumulation Using a Slope Shaking Table Test during Earthquake Action
Authors: Xu Jianmin , Jia Yuyue , Liu Junwei , Asheghabadi Mohsen Saleh , Wang Yulin
Published: December 2023

To examine the effects of different peak accelerations on the stability of the accumulation slope and the effectiveness of anti-slide piles under seismic loads, this paper used the Fanlingqian landslide as the main research object and combined it with digital image correlation (DIC) technology in order to carry out a shaking table test. Then, the acceleration response, displacement field, strain field, the bending moment distribution of the 0.05–0.3 g ground motion accumulation slope, and the anti-slide pile reinforcement were studied. The results of the test show the following: the amplification coefficient of the measuring points A1–A6 of the accumulation slope reaches the maximum at a peak acceleration of 0.2 g, and its values are between 1.25 and 1.3, respectively. Finally, it shows a decreasing trend at a peak acceleration of 0.3 g, and its corresponding values are, respectively, between 1.1 and 1.2. In the anti-slip pile reinforcement test, due to the obstruction of the anti-slip pile, the damping of the soil around the pile increases. As the peak value of the seismic wave input increases, the amplification factor shows an overall decreasing trend. A1–A6 correspond to a peak acceleration of 0.3 g. The amplification factors are all close to 1. During different peak accelerations, the accumulation slope mainly experienced the earthquake-induced stage, tensile failure stage, creeping deformation stage, and overall instability stage. In the anti-slide pile reinforcement test, under the same conditions, the slope mainly experienced the earthquake-induced stage, tensile failure stage, lower sliding surface formation stage, and soil shedding stage in front of the pile. At the same time, the displacement and strain fields of each stage of the two groups of tests are compared, and it is found that the displacement and strain values of the accumulation slope test are greater than those of the anti-slide pile reinforcement test, and the horizontal displacement difference at the top of the slope is the most significant, reaching 2.3 times at the maximum. The bending moment of the anti-slide pile first increases and then decreases with the increase in acceleration, the reverse bending point of the pile appears at 5 times the pile diameter below the soil surface, and the maximum bending moment of the middle pile, corresponding to a peak acceleration of 0.05–0.3 g, is between 7.5 N·m and 47 N·m, respectively, while the maximum bending moment of the outer pile is between 6.5 N·m and 52 N·m, respectively. It is important to apply DIC image processing technology to the monitoring of landslide structure and the evaluation of slope stability in practical engineering.
Highly Efficient and Magnetically Recyclable Non-Noble Metal Fly Ash-Based Catalysts for 4-Nitrophenol Reduction
Authors: Kuźniarska-Biernacka Iwona , Ferreira Inês , Monteiro Marta , Santos Ana Cláudia , Valentim Bruno , Guedes Alexandra , Belo João H. , Araújo João P. , Freire Cristina , Peixoto Andreia F.
Published: December 2023

4-nitrophenol (4-NPh) is a harmful compound produced in large amounts in the chemical industry, and its reduction to aminophenol (4-APh) using noble metals as catalysts is one of the most studied processes. The development of noble metal-free catalysts represents an economic advantage in large-scale applications and contributes to the sustainability of raw materials. Coal fly ash (FA), a major waste stream from coal combustion, contains an easily recoverable magnetic fraction (FAmag sample) composed of Fe-rich particles that could substitute noble metal catalysts in 4-NPh reduction, with the concomitant advantage of being easily recovered via magnetic separation. For this purpose, a new composite material containing copper ferrite nanoparticles (FAmag@CS@CuFe) was prepared via a facile, environmentally friendly and cost-effective method based on three components: FAmag as the core, a biobased polymer chitosan (CS) as the linker and copper ferrite CuFe2O4 nanoparticles (CuFe) as the active sites. The structure, morphology, composition and magnetic properties of the FAmag@CS@CuFe material were studied to assess the efficiency of the preparation. It was found that the biopolymer prevented the aggregation of CuFe nanoparticles and enabled a synergistically outstanding activity towards the reduction of 4-NPh in comparison to the pristine FAmag and bare CuFe nanoparticles. The FAmag@CS@CuFe catalyst showed efficiency and stability in the conversion of 4-NPh of up to 95% in 3 min over four consecutive cycles. Such remarkable catalytic results demonstrate the potential of this catalyst as a substitute for expensive noble metals.
A Comprehensive Review of the Potential Role of Liquid Biopsy as a Diagnostic, Prognostic, and Predictive Biomarker in Pancreatic Ductal Adenocarcinoma
Authors: Stosic Kosta , Senar Oier Azurmendi , Tarfouss Jawad , Bouchart Christelle , Navez Julie , Van Laethem Jean-Luc , Arsenijevic Tatjana
Published: December 2023

Pancreatic ductal adenocarcinoma is one of the most lethal malignant diseases, with a mortality rate being close to incidence. Due to its heterogeneity and plasticity, as well as the lack of distinct symptoms in the early phases, it is very often diagnosed at an advanced stage, resulting in poor prognosis. Traditional tissue biopsies remain the gold standard for making a diagnosis, but have an obvious disadvantage in their inapplicability for frequent sampling. Blood-based biopsies represent a non-invasive method which potentially offers easy and repeated sampling, leading to the early detection and real-time monitoring of the disease and hopefully an accurate prognosis. Given the urgent need for a reliable biomarker that can estimate a patient’s condition and response to an assigned treatment, blood-based biopsies are emerging as a potential new tool for improving patients’ survival and surveillance. In this article, we discuss the current advances and challenges in using liquid biopsies for pancreatic cancer, focusing on circulating tumour DNA (ctDNA), extracellular vesicles (EVs), and circulating tumour cells (CTCs), and compare the performance and reliability of different biomarkers and combinations of biomarkers.
Effects of Photoinitiators on Curing Performance of Wood Wax Oil Coating on Wood
Authors: Zhang Dan , Song Kuiyan
Published: December 2023

With the increasing shortage of petroleum resources and the growing seriousness of environmental pollution, the exploitation and application of bio-based coatings derived from renewable resources have become increasingly important for the woodworking industry. Wood wax oil (WWO) is a new type of bio-based natural coating material that offers an eco-friendly solution for wood protection. This paper focused on the utilization of tung oil and beeswax as the primary raw materials for the preparation of wood wax oil. The WWO was based on the oxidation polymerization of tung oil, which served as the foundation for the preparation process. The effects of the photoinitiator TPO-L on the curing performance of the WWO were investigated, and the curing mechanism of the WWO system induced by photoinitiators was analyzed and characterized by infrared spectroscopy. Through ultraviolet irradiation experiments and coating quality tests, the effects of incremental photoinitiators on the properties of the surface drying time, gloss, color, hydrophobicity, and solution resistance of the treated ash wood were studied. The results indicated that the addition of photoinitiators was beneficial for the rapid polymerization of wood wax oil. A UV light intensity of 30 w was found to be sufficient to initiate the curing process. Specifically, when using TPO-L as the initiator at a concentration of 3 wt%, the surface could be surface-dried within 10 min under UV exposure. Under these curing conditions, wood wax oil coatings based on tung oil with comprehensive curing properties can be obtained. Additionally, adding 6% beeswax to the tung oil can effectively enhance the hydrophobicity of pure tung-oil-based wood protective coatings.
SrTiO3 Thin Films on Dielectric Substrates for Microwave Applications
Authors: Tumarkin Andrey , Sapego Eugene , Gagarin Alexander , Bogdan Alexey , Karamov Artem , Serenkov Igor , Sakharov Vladimir
Published: December 2023

Thin films of strontium titanate, which have nonlinear properties that are promising for microwave applications, were grown on a polycrystalline aluminum oxide substrate using magnetron sputtering and high-temperature annealing. It was shown that the improvement of the film structure with an increase in the deposition temperature was clearly correlated with both an increase in nonlinearity and an improvement in the loss level. A capacitor based on an SrTiO3 film deposited at a deposition temperature of 900 °C and subjected to annealing demonstrated a tunability of 46% with a loss tangent of 0.009–0.014 at a frequency of 2 GHz. This was the first successful attempt to form a planar SrTiO3 capacitor on an alumina substrate, which exhibited a commutation quality factor of above 3000 in the microwave range.
Cross-Attention and Seamless Replacement of Latent Prompts for High-Definition Image-Driven Video Editing
Authors: Zhao Liangbing , Zhang Zicheng , Nie Xuecheng , Liu Luoqi , Liu Si
Published: December 2023

Recently, text-driven video editing has received increasing attention due to the surprising success of the text-to-image model in improving video quality. However, video editing based on the text prompt is facing huge challenges in achieving precise and controllable editing. Herein, we propose Latent prompt Image-driven Video Editing (LIVE) with a precise and controllable video editing function. The important innovation of LIVE is to utilize the latent codes from reference images as latent prompts to rapidly enrich visual details. The novel latent prompt mechanism endows two powerful capabilities for LIVE: one is a comprehensively interactive ability between video frame and latent prompt in the spatial and temporal dimensions, achieved by revisiting and enhancing cross-attention, and the other is the efficient expression ability of training continuous input videos and images within the diffusion space by fine-tuning various components such as latent prompts, textual embeddings, and LDM parameters. Therefore, LIVE can efficiently generate various edited videos with visual consistency by seamlessly replacing the objects in each frame with user-specified targets. The high-definition experimental results from real-world videos not only confirmed the effectiveness of LIVE but also demonstrated important potential application prospects of LIVE in image-driven video editing.
Analysis of Small-Disturbance Stability of Onshore Wind Power All-DC Power Generation System and Identification of Leading Factors
Authors: Jin Guixin , Li Fengting , Yin Chunya , Liu Jiangshan , Wang Tao
Published: December 2023

The application of conventional AC collection for the integration of large-scale renewable energy sources may lead to issues concerning harmonic resonance and reactive power transmission. Conversely, the utilization of an all-DC power generation system for wind power (WDCG) can effectively circumvent such issues. In contrast to the conventional power system, the interdependence among subsystems in the WDCG renders it susceptible to oscillation instability in the presence of minor disturbances. To address this concern, this paper first establishes a small-signal model for the WDCG, and validates the accuracy of this model by comparing it with an electromagnetic transient model based on PSCAD/EMTDC. Secondly, employing the eigenvalue analysis method, the principal oscillation modes of the WDCG are identified, and the state variables strongly correlated with these modes are analyzed using the participation factor method. Moreover, a quantitative assessment of the impact of operational and control parameters closely associated with the strongly correlated state variables on the negative damper oscillation model is conducted. The findings of the analysis reveal that the small-disturbance stability of the WDCG is significantly influenced by the operational parameters of the outlet capacitance of the ma-chine-side converter (MSC), the outlet capacitance of the direct current wind turbine (DCWT), the sub-module capacitance of the modular multilevel converter (MMC), and the inductance of the bridge arm. Additionally, the stability is al-so affected by the control parameters of the constant DC voltage control on the DCWT side, the voltage outer-loop–current inner-loop control, and the circulation suppression on the MMC side. The simulation results based on PSCAD validate the efficacy of the proposed method in identifying the dominant factors.
Concealed Inherited Cardiomyopathies Detected in Cardio-Oncology Screening
Authors: Lorca Rebeca , Pascual Isaac , Fernandez Maria , Alvarez-Velasco Rut , Colunga Santiago , Muñiz Maria , Izquierdo Marta , Fernandez Yolanda , Esteban Emilio , Gomez Juan , Avanzas Pablo , Lopez-Fernandez Teresa
Published: December 2023

Introduction: Basal cardiovascular risk assessment in cardio-oncology is essential. Integrating clinical information, ECG and transthoracic echocardiogram can identify concealed inherited cardiomyopathies (ICMPs) with potential added risk of cardiotoxicity. We aimed to evaluate the impact of our Cardio-Oncology Unit design in detecting concealed ICMPs. Methods: We carried out a retrospective study of all consecutive breast cancer patients referred to the Cardio-Oncology Unit for cardiac evaluation (2020–2022). ICMPs diagnosis was provided according to ESC guidelines and underwent genetic testing. ICMPs prevalence in this cohort was compared to the highest and lowest frequency reported in the general population. Results: Among 591 breast cancer patients, we identified eight patients with ICMPs: one arrhythmogenic cardiomyopathy (ACM), three familial non-ischemic dilated cardiomyopathy (DCM), three hypertrophic cardiomyopathy (HCM) and one left ventricular non-compaction cardiomyopathy (LVNC), which has now been reclassified as non-dilated left ventricular cardiomyopathy. The number of ICMPs identified was within the expected range (neither overdiagnosed nor overlooked): ACM 0.0017 vs. 0.0002–0.001 (p 0.01–0.593); DCM 0.0051 vs. 0.002–0.0051 (p 0.094–0.676); HCM 0.005 vs. 0.0002–0.002 (p < 0.001–0.099); LVCN 0.0017 vs. 0.00014–0.013 (p 0.011–0.015). Genetic testing identified a pathogenic FLNC variant and two pathogenic TTN variants. Conclusion: Opportunistic screening of ICMPs during basal cardiovascular risk assessment can identify high-risk cancer patients who benefit from personalized medicine and enables extension of prevention strategies to all available relatives at concealed high cardiovascular risk.
Creatinine Reduction Ratio Is a Prognostic Factor for Acute Kidney Injury following Cardiac Surgery with Cardiopulmonary Bypass: A Single-Center Retrospective Cohort Study
Authors: Anzai Akiko , Takaki Shunsuke , Yokoyama Nobuyuki , Kashiwagi Shizuka , Yokose Masashi , Goto Takahisa
Published: December 2023

Acute kidney injury (AKI) after cardiac surgery is a common complication that can lead to death. We previously reported that the creatinine reduction ratio (CRR) serves as a useful prognostic factor for AKI. The primary objective of this study was to determine the predictors of AKI after surgery. The secondary objective was to determine the reliability of the CRR for short- and long-term outcomes. We retrospectively collected information about cardiac surgery patients who underwent cardiopulmonary bypass. Patients were divided into AKI and non-AKI groups based on the AKIN and RIFLE criteria. We analyzed the two groups regarding the preoperative patient data and operative information. The CRR was calculated as follows: (preoperative creatinine—postoperative creatinine)/preoperative creatinine. The prognostic factors of AKI-CS were surgery time, CPB time, aorta clamp time, platelet transfusion, and CRR < 20%. In the multivariate logistical analysis, CRR was an independent predictor of AKI (adjusted odds ratio: 0.90 [0.87–0.93], p < 0.001). However, there were no significant differences in CRR in terms of the rate of new onset chronic kidney disease (CKD). After cardiac surgery with cardiopulmonary bypass, CRR has good diagnostic power for predicting perioperative AKI. However, we cannot use it as a prognostic factor over a long-term period.
Prevention and Management of Infections in Lung Transplant Recipients
Authors: Fayyaz Anum , Raja Mohammed , Natori Yoichiro
Published: December 2023

Anti-rejection medications are essential in preventing organ rejection amongst solid organ transplant recipients; however, these agents also cause profound immunosuppression, predisposing lung transplant recipients (LTRs) to infectious complications. The timely management including prevention, diagnosis, and treatment of such infectious complications is vital to prevent significant morbidity and mortality in solid organ transplant recipients and allograft dysfunction. LTRs are inundated with microbes that may be recognized as commensals in hosts with intact immune systems. Bacterial infections are the most common ones, followed by viral pathogens. Indications of a brewing infectious process may be subtle. Hence, the importance of adapting vigilance around isolated hints through symptomatology and signs is pivotal. Signals to suggest an infectious process, such as fever and leukocytosis, may be dampened by immunosuppressive agents. One must also be vigilant about drug interactions of antibiotics and immunosuppressive agents. Treatment of infections can become challenging, as antimicrobials can interact with immunosuppressive agents, and antimicrobial resistance can surge under antimicrobial pressure. Transplant infectious disease physicians work in concert with transplant teams to obtain specimens for diagnostic testing and follow through with source control when possible. This heavily impacts medical decisions and fosters a multidisciplinary approach in management. Furthermore, the reduction of immunosuppression, although it augments the risk of allograft rejection, is as crucial as the initiation of appropriate antimicrobials when it comes to the management of infections.
Three-Dimensional Printing of Biomass–Fungi Biocomposite Materials: The Effects of Mixing and Printing Parameters on Fungal Growth
Authors: Rahman Al Mazedur , Bhardwaj Abhinav , Vasselli Joseph G. , Pei Zhijian , Shaw Brian D.
Published: December 2023

Biomass–fungi biocomposite materials are derived from sustainable sources and can biodegrade at the end of their service. They can be used to manufacture products that are traditionally made from petroleum-based plastics. There are potential applications for these products in the packaging, furniture, and construction industries. In the biomass–fungi biocomposite materials, the biomass particles (made from agricultural waste such as hemp hurd) act as the substrate, and a network of fungal hyphae grow through and bind the biomass particles together. Typically, molding-based methods are used to manufacture products using these biocomposite materials. Recently, the authors reported a novel extrusion-based 3D printing method using these biocomposite materials. This paper reports a follow-up investigation into the effects of mixing parameters (mixing time and mixing mode) on fungal growth in biomass–fungi mixtures prepared for 3D printing and the effects of printing parameters (printing speed and extrusion pressure) on fungal growth in printed samples. The fungal growth was quantified using the number of fungal colonies that grew from samples. The results show that, when mixing time increased from 15 to 120 s, there was a 52% increase in fungal growth. Changing from continuous to intermittent mixing mode resulted in an 11% increase in fungal growth. Compared to mixtures that were not subjected to printing, samples printed with a high printing speed and high extrusion pressure had a 14.6% reduction in fungal growth, while those with a low printing speed and low extrusion pressure resulted in a 16.5% reduction in fungal growth.
A Curcumin-Based Oral Gel Has Potential Protective Efficacy against Oral Mucositis: In Vitro Study
Authors: Idrees Majdy , Kujan Omar
Published: December 2023

Oral mucositis is a common distressing complication of cancer therapy, characterised by painful sores within the oral cavity. Current management options offer limited symptomatic relief. Curcumin, a natural polyphenolic compound with recognised anti-inflammatory and antioxidant properties, has emerged as a potential protective agent against oral mucositis. This study explores the therapeutic potential of curcumin in mitigating the impact of oral mucositis by investigating a commercially available curcumin-based oral gel, PerioGold®. Liquid chromatography–tandem mass spectrometry was used to characterise the main constituents of PerioGold®. The cytotoxicity of curcumin constituent was investigated in four cell lines: primary oral keratinocytes (HOKs), immortalised oral keratinocytes (OKF6), dysplastic oral keratinocytes (DOKs), and oral squamous cell carcinoma cells (PE/CA-PJ15). Concentrations of hydrogen peroxide were optimised to develop in vitro models that mimic oral mucositis. The inhibitory effects of PerioGold® against the production of reactive oxygen species were assessed using a designated kit. OKF6 cells were the most sensitive to oxidative stress, while PE/CA-PJ15 cells showed the highest resistance. Pretreatment of the investigated cells for 24 h with PerioGold® demonstrated a significant antioxidative effect in all cells based on a dose–response pattern. PerioGold® exhibits clinical potential for protecting against oxidative stress, warranting further individualised clinical investigations.
Real-Life Effects of Omalizumab on Chronic Rhinosinusitis with Nasal Polyposis
Authors: Lombardo Nicola , Piazzetta Giovanna Lucia , Lobello Nadia , Cicala Giuseppe , Patafi Maria , Benincasa Anna Teresa , Pelaia Corrado , Chiarella Emanuela , Pelaia Girolamo
Published: December 2023

Chronic rhinosinusitis with nasal polyposis (CRSwNP) is an inflammatory disease of the nasal and sinus mucosa. This inflammatory process is supported by a multitude of cytokines, including IL-4, IL-5, and IL-13 produced by Th2 cells, as well as by IgE produced by B lymphocytes in response to a stimulus. Omalizumab is an anti-IgE monoclonal antibody with well-recognized roles in allergic asthma and chronic spontaneous urticaria. The aim of this study was to evaluate the clinical efficacy of omalizumab in a cohort of 13 patients suffering from chronic rhinosinusitis with CRSwNP. The inclusion criteria considered were as follows: 18 years of age, with a diagnosis of chronic rhinosinusitis with severe nasal polyposis expressed by an NPS greater than or equal to 5 and/or a SNOT-22 greater than or equal to 50. In addition, in the enrolled patients, the classic treatment with corticosteroids had to have been suspended due to recurrence after surgery or lack of response. Our results highlighted that omalizumab treatment for 16 weeks improved the parameters analyzed: SNOT-22, NPS, NRS, and NCS. The clinical efficacy of omalizumab was further strengthened by a significant improvement in respiratory function as well as reductions in the nasal polyps’ size and in the associated symptoms.
Enhancing Access to Urban Hill Parks: The Montjuïc Trail Masterplan and the 360° Route Design in Barcelona
Authors: Mercadé-Aloy Josep , Cervera-Alonso-de-Medina Marina
Published: December 2023

The 2030 United Nations Sustainable Development Goals (SDGs) include ensuring universal and safe access to green spaces. Some cities feature extensive green areas on hills or elevated terrains integrated into the urban landscape. In such cases where the benefits for users are highly pronounced (e.g., views, isolation, etc.), it is challenging and particularly complex to design strategies to ensure accessible and spatial routes due to multiple slopes and a challenging topography. In Barcelona, the iconic Montjuïc mountain has been the focal point of a trail masterplan aimed at rethinking its various access points and internal network of routes. Furthermore, the city has committed to implementing an initial project from this plan, the so-called 360° route. This study presents an in-depth analysis of the Montjuïc mountain case, encompassing both the plan and the 360° project in hilly urban parks. The analysis reveals the values and transferability of the set of strategies proposed in the plan, such as activating inherent location characteristics by connecting the surrounding urban fabric with elements of recreational potential within the underlying traces of heritage value. Additionally, a quantitative assessment of the impact of the proposed accesses on the population is presented. The study highlights the improvements in quality of life for the diverse users of this type of green infrastructure.
Assessing the Cumulative Impacts of Forest Management on Forest Age Structure Development and Woodland Caribou Habitat in Boreal Landscapes: A Case Study from Two Canadian Provinces
Authors: Mackey Brendan , Campbell Carly , Norman Patrick , Hugh Sonia , DellaSala Dominick A. , Malcolm Jay R. , Desrochers Mélanie , Drapeau Pierre
Published: December 2023

The Canadian boreal forest biome has been subjected to a long history of management for wood production. Here, we examined the cumulative impacts of logging on older forests in terms of area, distribution and patch configuration in the managed forest zones of the Eastern Canadian provinces of Ontario and Quebec. We also examined the consequences of these cumulative impacts on a once widely distributed and now threatened species, the woodland caribou (Rangifer tarandus caribou). The cumulative area of recently logged forest (since ~1976) was 14,024,619 ha, with 8,210,617 ha in Quebec and 5,814,002 ha in Ontario. The total area of older forests was 21,249,341 ha, with 11,840,474 ha in Quebec and 9,408,867 ha in Ontario. Patch statistics revealed that there were 1,085,822 older forests with core patches < 0.25 ha and an additional 603,052 < 1.0 ha. There were 52 > 10,00–50,000 ha and 8 < 50,000 ha. Older forest patches (critical caribou habitat) in the 21 local population ranges totalled 6,103,534 ha, distributed among ~387,102 patches with 362,933 < 10 ha and 14 > 50,000 ha. The median percentage of local population ranges that was disturbed was 53.5%, with Charlevoix having the maximum (90.3%) and Basse Côte-Nord the least (34.9%). Woodland caribou local population ranges with disturbed suitable habitats >35% are considered unable to support self-sustaining populations. We found that for the 21 caribou local population ranges examined, 3 were at very high risk (>75% area disturbed), 16 at high risk (>45 ≤ 75% area disturbed), and 2 at low risk (≤35% area disturbed). Major changes are needed in boreal forest management in Ontario and Quebec for it to be ecologically sustainable, including a greater emphasis on protection and restoration for older forests, and to lower the risks for caribou populations.
Prediction of Clinical Remission with Adalimumab Therapy in Patients with Ulcerative Colitis by Fourier Transform–Infrared Spectroscopy Coupled with Machine Learning Algorithms
Authors: Kim Seok-Young , Shin Seung Yong , Saeed Maham , Ryu Ji Eun , Kim Jung-Seop , Ahn Junyoung , Jung Youngmi , Moon Jung Min , Choi Chang Hwan , Choi Hyung-Kyoon
Published: December 2023

We aimed to develop prediction models for clinical remission associated with adalimumab treatment in patients with ulcerative colitis (UC) using Fourier transform–infrared (FT–IR) spectroscopy coupled with machine learning (ML) algorithms. This prospective, observational, multicenter study enrolled 62 UC patients and 30 healthy controls. The patients were treated with adalimumab for 56 weeks, and clinical remission was evaluated using the Mayo score. Baseline fecal samples were collected and analyzed using FT–IR spectroscopy. Various data preprocessing methods were applied, and prediction models were established by 10-fold cross-validation using various ML methods. Orthogonal partial least squares–discriminant analysis (OPLS–DA) showed a clear separation of healthy controls and UC patients, applying area normalization and Pareto scaling. OPLS–DA models predicting short- and long-term remission (8 and 56 weeks) yielded area-under-the-curve values of 0.76 and 0.75, respectively. Logistic regression and a nonlinear support vector machine were selected as the best prediction models for short- and long-term remission, respectively (accuracy of 0.99). In external validation, prediction models for short-term (logistic regression) and long-term (decision tree) remission performed well, with accuracy values of 0.73 and 0.82, respectively. This was the first study to develop prediction models for clinical remission associated with adalimumab treatment in UC patients by fecal analysis using FT–IR spectroscopy coupled with ML algorithms. Logistic regression, nonlinear support vector machines, and decision tree were suggested as the optimal prediction models for remission, and these were noninvasive, simple, inexpensive, and fast analyses that could be applied to personalized treatments.
Study on Synergistic Strengthening of Gold Extraction with Copper Ethylenediamine Thiosulfate Using Pyrite and Nickel Ions
Authors: Qin Xuecong , Zhang Tao , Zi Futing , Zhang Hongbo , Li Guoping
Published: December 2023

Gold leaching using the copper–ethylenediamine–thiosulfate (Cu2+-en-S2O32−) system, which contains copper–ethylenediamine complexes, instead of the use of copper–ammonia catalysis, is environmentally friendly and cost-effective. In this study, pyrite and Ni2+ were added to the Cu2+-en-S2O32− system to clarify their individual and combined influence on gold leaching. The result obtained showed that when pyrite and Ni2+ were separately added to the system, the dissolution of gold was significantly inhibited. However, the disappearance of the negative impacts of these two substances when they were simultaneously added to the system revealed that they exhibited a synergistic effect on gold dissolution. Notably, Ni2+ weakened the promotional effect of pyrite on the formation of a Cu-containing passivation layer on the gold surface. Furthermore, the separate addition of Ni2+ and pyrite increased the corrosion potential of gold; thus, gold dissolution was inhibited. However, when added together, they brought about a decrease in the corrosion potential of gold, while increasing its dissolution rate. These findings provide a reference for the efficient extraction of pyrite-associated gold, which can be applied to improve the green extraction process of gold.
Electrochemiluminescence Sensor Based on CeO2 Nanocrystalline for Hg2+ Detection in Environmental Samples
Authors: Tian Chunyuan , Tang Feiyan , Guo Wei , Wei Minggang , Wang Li , Zhuang Xuming , Luan Feng
Published: December 2023

The excessive concentration of heavy-metal mercury ions (Hg2+) in the environment seriously affects the ecological environment and even threatens human health. Therefore, it is necessary to develop rapid and low-cost determination methods to achieve trace detection of Hg2+. In this paper, an Electrochemiluminescence (ECL) sensing platform using a functionalized rare-earth material (cerium oxide, CeO2) as the luminescent unit and an aptamer as a capture unit was designed and constructed. Using the specific asymmetric matching between Hg2+ and thymine (T) base pairs in the deoxyribonucleic acid (DNA) single strand, the “T−Hg−T” structure was formed to change the ECL signal, leading to a direct and sensitive response to Hg2+. The results show a good linear relationship between the concentration and the response signal within the range of 10 pM–100 µM for Hg2+, with a detection limit as low as 0.35 pM. In addition, the ECL probe exhibits a stable ECL performance and excellent specificity for identifying target Hg2+. It was then successfully used for spiked recovery tests of actual samples in the environment. The analytical method solves the problem of poor Hg2+ recognition specificity, provides a new idea for the efficient and low-cost detection of heavy-metal pollutant Hg2+ in the environment, and broadens the prospects for the development and application of rare-earth materials.
Design and Construction of Enzyme-Based Electrochemical Gas Sensors
Authors: Zhang Wenjian , Chen Xinyi , Xing Yingying , Chen Jingqiu , Guo Lanpeng , Huang Qing , Li Huayao , Liu Huan
Published: December 2023

The demand for the ubiquitous detection of gases in complex environments is driving the design of highly specific gas sensors for the development of the Internet of Things, such as indoor air quality testing, human exhaled disease detection, monitoring gas emissions, etc. The interaction between analytes and bioreceptors can described as a “lock-and-key”, in which the specific catalysis between enzymes and gas molecules provides a new paradigm for the construction of high-sensitivity and -specificity gas sensors. The electrochemical method has been widely used in gas detection and in the design and construction of enzyme-based electrochemical gas sensors, in which the specificity of an enzyme to a substrate is determined by a specific functional domain or recognition interface, which is the active site of the enzyme that can specifically catalyze the gas reaction, and the electrode–solution interface, where the chemical reaction occurs, respectively. As a result, the engineering design of the enzyme electrode interface is crucial in the process of designing and constructing enzyme-based electrochemical gas sensors. In this review, we summarize the design of enzyme-based electrochemical gas sensors. We particularly focus on the main concepts of enzyme electrodes and the selection and design of materials, as well as the immobilization of enzymes and construction methods. Furthermore, we discuss the fundamental factors that affect electron transfer at the enzyme electrode interface for electrochemical gas sensors and the challenges and opportunities related to the design and construction of these sensors.
Diversity of Self-Assembled RNA Complexes: From Nanoarchitecture to Nanomachines
Authors: Kanarskaya Maria A. , Pyshnyi Dmitrii V. , Lomzov Alexander A.
Published: December 2023

New tool development for various nucleic acid applications is an essential task in RNA nanotechnology. Here, we determined the ability of self-limited complex formation by a pair of oligoribonucleotides carrying two pairwise complementary blocks connected by a linker of different lengths in each chain. The complexes were analyzed using UV melting, gel shift assay analysis, and molecular dynamics (MD) simulations. We have demonstrated the spontaneous formation of various self-limited and concatemer complexes. The linear concatemer complex is formed by a pair of oligomers without a linker in at least one of them. Longer linkers resulted in the formation of circular complexes. The self-limited complexes formation was confirmed using the toehold strand displacement. The MD simulations indicate the reliability of the complexes’ structure and demonstrate their dynamics, which increase with the rise of complex size. The linearization of 2D circular complexes into 1D structures and a reverse cyclization process were demonstrated using a toehold-mediated approach. The approach proposed here for the construction and directed modification of the molecularity and shape of complexes will be a valuable tool in RNA nanotechnology, especially for the rational design of therapeutic nucleic acids with high target specificity and the programmable response of the immune system of organisms.
Selective Wine Aroma Enhancement through Enzyme Hydrolysis of Glycosidic Precursors
Authors: Rodríguez-Nogales José Manuel , Fernández-Fernández Encarnación , Ruipérez Violeta , Vila-Crespo Josefina
Published: December 2023

Selective enhancement of wine aroma was achieved using a broad spectrum of exogenous glycosidases. Eight different enzyme preparations were added to Verdejo wine, resulting in an increase in the levels of varietal volatile compounds compared to the control wine after 15 days of treatment. The enzyme preparations studied were robust under winemaking conditions (sulfur dioxide, reducing sugars, and alcohol content), and no inhibition of β-glucosidase activity was observed. Significant differences were detected in four individual terpenes (α-terpineol, terpinen-4-ol, α-pinene, and citronellal) and benzyl alcohol in all the treated wines compared to the control wine, contributing to the final wine to varying degrees. In addition, a significant increase in the other aromatic compounds was observed, which showed different patterns depending on the enzyme preparation that was tested. The principal component analysis of the data revealed the possibility of modulating the different aromatic profiles of the final wines depending on the enzyme preparation used. Taking these results into account, enhancement of the floral, balsamic, and/or fruity notes of wines is possible by using a suitable commercial enzyme preparation.
Synthesis and Characterization of Ruthenium-Paraphenylene-Cyclopentadienyl Full-Sandwich Complexes: Cytotoxic Activity against A549 Lung Cancer Cell Line and DNA Binding Properties
Authors: Sifnaiou Evangelia , Tsolis Theodoros , Ypsilantis Konstantinos , Roupakia Eugenia , Kolettas Evangelos , Plakatouras John C. , Garoufis Achilleas
Published: December 2023

Novel full-sandwich (η5-Cp)-Ru-paraphenylene complexes with the general formula [(η5-Cp)nRu(η6-L)](PF6)n where n = 1–3 and L = biphenyl, p-terphenyl and p-quaterphenyl, were synthesized and characterized by means of spectroscopic and analytical techniques. The structures of the complexes [(η5-Cp)Ru(η6-biphenyl)](PF6) (1), [(η5-Cp)Ru(η6-terphenyl)](PF6) (3) and [(η5-Cp)2Ru(η6-terphenyl)](PF6)2 (4) was determined by X-ray single crystal methods. The interaction of the complexes [(η5-Cp)Ru(η6-quaterphenyl)]Cl, (6)Cl, and [(η5-Cp)2Ru(η6-quaterphenyl)]Cl2, (7)Cl2, with the DNA duplex d(5′-CGCGAATTCGCG-3′)2 was studied using NMR techniques. The results showed that both complexes interacted non-specifically with both the minor and major grooves of the helix. Specifically, (6)Cl exhibited partial binding through intercalation between the T7 and T8 bases of the sequence without disrupting the C–G and A–T hydrogen bonds. Fluorometric determination of the complexes’ binding constants revealed a significant influence of the number of connected phenyl rings in the paraphenylene ligand (L) on the binding affinity of their complexes with the d(5′-CGCGAATTCGCG-3′)2. The complexes (6)Cl and (7)Cl2 were found to be highly cytotoxic against the A549 lung cancer cell line, with complex (6) being more effective than (7) (IC50 for (6)Cl: 17.45 ± 2.1 μΜ, IC50 for (7)Cl2: 65.83 ± 1.8 μΜ) and with a selectivity index (SI) (SI for (6)Cl: 1.1 and SI for (7)Cl2: 4.8).
Computational 3D Modeling-Based Identification of Inhibitors Targeting Cysteine Covalent Bond Catalysts for JAK3 and CYP3A4 Enzymes in the Treatment of Rheumatoid Arthritis
Authors: Faris Abdelmoujoud , Alnajjar Radwan , Guo Jingjing , AL Mughram Mohammed H. , Aouidate Adnane , Asmari Mufarreh , Elhallaoui Menana
Published: December 2023

This work aimed to find new inhibitors of the CYP3A4 and JAK3 enzymes, which are significant players in autoimmune diseases such as rheumatoid arthritis. Advanced computer-aided drug design techniques, such as pharmacophore and 3D-QSAR modeling, were used. Two strong 3D-QSAR models were created, and their predictive power was validated by the strong correlation (R2 values > 80%) between the predicted and experimental activity. With an ROC value of 0.9, a pharmacophore model grounded in the DHRRR hypothesis likewise demonstrated strong predictive ability. Eight possible inhibitors were found, and six new inhibitors were designed in silico using these computational models. The pharmacokinetic and safety characteristics of these candidates were thoroughly assessed. The possible interactions between the inhibitors and the target enzymes were made clear via molecular docking. Furthermore, MM/GBSA computations and molecular dynamics simulations offered insightful information about the stability of the binding between inhibitors and CYP3A4 or JAK3. Through the integration of various computational approaches, this study successfully identified potential inhibitor candidates for additional investigation and efficiently screened compounds. The findings contribute to our knowledge of enzyme–inhibitor interactions and may help us create more effective treatments for autoimmune conditions like rheumatoid arthritis.
Pullulan-Graft-Polyoxazoline: Approaches from Chemistry and Physics
Authors: Zorin Ivan M. , Fetin Petr A. , Mikusheva Nina G. , Lezov Alexey A. , Perevyazko Igor , Gubarev Alexander S. , Podsevalnikova Anna N. , Polushin Sergey G. , Tsvetkov Nikolai V.
Published: December 2023

An approach to the preparation of pullulan-graft-poly(2-methyl-2-oxazoline)s based on Cu-catalyzed azide–alkyne cycloaddition with polyoxazoline-azide was applied. All of the obtained polymers were characterized through classical molecular hydrodynamic methods and NMR. The formation of graft copolymers was accomplished by oxidative degradation of pullulan chains. Nevertheless, graft copolymers were obtained as uniform products with varied side chain lengths and degrees of substitution.