bluuebunny/crossref_metadata_2025 huggingface dataset:
The thermal sensation threshold and its reliability induced by the exposure to 28 GHz millimeter-wave
Authors: Yuasa Akiko , Uehara Shintaro , Ushizawa Kazuki , Kodera Sachiko , Arai Norika , Hirata Akimasa , Otaka Yohei
Published: February 2024

The application of 28 GHz millimeter-wave is prevalent owing to the global spread of fifth-generation wireless communication systems. Its thermal effect is a dominant factor which potentially causes pain and tissue damage to the body parts exposed to the millimeter waves. However, the threshold of this thermal sensation, that is, the degree of change in skin temperature from the baseline at which the first subjective response to the thermal effects of the millimeter waves occurs, remains unclear. Here, we investigated the thermal sensation threshold and assessed its reliability when exposed to millimeter waves. Twenty healthy adults were exposed to 28 GHz millimeter-wave on their left middle fingertip at five levels of antenna input power: 0.2, 1.1, 1.6, 2.1, and 3.4 W (incident power density: 27–399 mW/cm2). This measurement session was repeated twice on the same day to evaluate the threshold reliability. The intraclass correlation coefficient (ICC) and Bland–Altman analysis were used as proxies for the relative and absolute reliability, respectively. The number of participants who perceived a sensation during the two sessions at each exposure level was also counted as the perception rate. Mean thermal sensation thresholds were within 0.9°C–1.0°C for the 126–399 mW/cm2 conditions, while that was 0.2°C for the 27 mW/cm2 condition. The ICCs for the threshold at 27 and 126 mW/cm2 were interpreted as poor and fair, respectively, while those at higher exposure levels were moderate to substantial. Apart from a proportional bias in the 191 mW/cm2 condition, there was no fixed bias. All participants perceived a thermal sensation at 399 mW/cm2 in both sessions, and the perception rate gradually decreased with lower exposure levels. Importantly, two-thirds of the participants answered that they felt a thermal sensation in both or one of the sessions at 27 mW/cm2, despite the low-temperature increase. These results suggest that the thermal sensation threshold is around 1.0°C, consistent across exposure levels, while its reliability increases with higher exposure levels. Furthermore, the perception of thermal sensation may be inherently ambiguous owing to the nature of human perception.
Investigating critical brain area for EEG-based binocular color fusion and rivalry with EEGNet
Authors: Lv Zhineng , Liu Xiang , Dai Mengshi , Jin Xuesong , Huang Xiaoqiao , Chen Zaiqing
Published: February 2024

Introduction

Binocular color fusion and rivalry are two specific phenomena in binocular vision, which could be used as experimental tools to study how the brain processes conflicting information. There is a lack of objective evaluation indexes to distinguish the fusion or rivalry for dichoptic color.

Methods

This paper introduced EEGNet to construct an EEG-based model for binocular color fusion and rivalry classification. We developed an EEG dataset from 10 subjects.

Results

By dividing the EEG data from five different brain areas to train the corresponding models, experimental results showed that: (1) the brain area represented by the back area had a large difference on EEG signals, the accuracy of model reached the highest of 81.98%, and more channels decreased the model performance; (2) there was a large effect of inter-subject variability, and the EEG-based recognition is still a very challenge across subjects; and (3) the statistics of EEG data are relatively stationary at different time for the same individual, the EEG-based recognition is highly reproducible for an individual.

Discussion

The critical channels for EEG-based binocular color fusion and rivalry could be meaningful for developing the brain computer interfaces (BCIs) based on color-related visual evoked potential (CVEP).
Association of soluble transferrin receptor/log ferritin index with all-cause and cause-specific mortality: National Health and Nutrition Examination Survey
Authors: Yu Yan , Lu Dongying , Zhang Zhenhui , Tao Lili
Published: February 2024

Background

Soluble transferrin receptor (sTfR)/log ferritin index (sTfR Index) can be used to assess the entire spectrum of iron status, and is valuable in evaluating iron status in population studies. There is still a lack of evidence on the association between sTfR index and all-cause mortality.

Object

To explore the association between sTfR index and all-cause mortality, as well as mortality due to cardiovascular disease (CVD) and cancer.

Method

Data were from the National Health and Nutrition Examination Survey (NHANES) between 2003 to 2020. Participants aged 16 years and older who had complete data of serum ferritin and sTfR were included. Pregnant individuals or those with ineligible data on death or follow-up were excluded from the analysis. Baseline sTfR index was calculated by baseline sTfR/log (ferritin) and classified as three tertile. We performed the Cox proportional hazard regression to assess the association of sTfR index (both continuous and categorical scale) with all-cause and cause-specific mortality and further assess the non-linear relationship between sTfR index and the outcomes with restricted cubic spline.

Result

In this study, 11,525 participants, a total of 231 (2.0%) all-cause deaths occurred during a median follow-up of 51 months. The risk of all-cause mortality, CVD-related mortality, and cancer-related mortality was higher in participants with highest tertile of sTfR index. After confounding factors adjustment, participants with highest tertile of sTfR index were associated with an increased risk of all-cause mortality (HR: 1.71, 95% CI: 1.14–2.57) as compared with lowest tertile. Additionally, sTfR index per SD increment was associated with a 25% increasing risk of all-cause mortality (HR: 1.25, 95% CI: 1.08–1.45, p = 0.003) and a 38% cancer-related mortality (HR: 1.38, 95% CI: 1.07–1.77, p = 0.018). These associations remained robust after adjusting for the serum ferritin as well as in various subgroups stratified by age, sex, smoking statue, hypertension, diabetes, and CVD. Spline analysis showed that there is approximately linear relationship between sTfR index with all-cause mortality (p for non-linear = 0.481). Moreover, ferritin was not a predictor of all-cause death after adjustment for confounding factors.

Significance

This cohort study demonstrated a significant association between sTfR index increment and an increased risk of all-cause and cancer-related mortality, independent of ferritin levels.
Apigenin: a natural molecule at the intersection of sleep and aging
Authors: Kramer Daniel J. , Johnson Adiv A.
Published: February 2024

NAD+, a pivotal coenzyme central to metabolism, exhibits a characteristic decline with age. In mice, NAD+ levels can be elevated via treatment with apigenin, a natural flavonoid that inhibits the NAD+-consuming glycoprotein CD38. In animal models, apigenin positively impacts both sleep and longevity. For example, apigenin improves learning and memory in older mice, reduces tumor proliferation in a mouse xenograft model of triple-negative breast cancer, and induces sedative effects in mice and rats. Moreover, apigenin elongates survival in fly models of neurodegenerative disease and apigenin glycosides increase lifespan in worms. Apigenin’s therapeutic potential is underscored by human clinical studies using chamomile extract, which contains apigenin as an active ingredient. Collectively, chamomile extract has been reported to alleviate anxiety, improve mood, and relieve pain. Furthermore, dietary apigenin intake positively correlates with sleep quality in a large cohort of adults. Apigenin’s electron-rich flavonoid structure gives it strong bonding capacity to diverse molecular structures across receptors and enzymes. The effects of apigenin extend beyond CD38 inhibition, encompassing agonistic and antagonistic modulation of various targets, including GABA and inflammatory pathways. Cumulatively, a large body of evidence positions apigenin as a unique molecule capable of influencing both aging and sleep. Further studies are warranted to better understand apigenin’s nuanced mechanisms and clinical potential.
Clinical application of machine learning‐based pathomics signature of gastric atrophy
Authors: Lan Yadi , Han Bing , Zhai Tianyu , Xu Qianqian , Li Zhiwei , Liu Mingyue , Xue Yining , Xu Hongwei
Published: February 2024

Background

The diagnosis of gastric atrophy is highly subjective, and we aimed to establish a model of gastric atrophy based on pathological features to improve diagnostic consistency.

Methods

We retrospectively collected the HE-stained pathological slides of gastric biopsies and used CellProfiler software for image segmentation and feature extraction of ten representative images for each sample. Subsequently, we employed the Least absolute shrinkage and selection operator (LASSO) to select features and different machine learning (ML) algorithms to construct the diagnostic models for gastric atrophy.

Results

We selected 289 gastric biopsy specimens for training, testing, and external validation. We extracted 464 pathological features and screened ten features by LASSO to establish the diagnostic model for moderate-to-severe atrophy. The range of area under the curve (AUC) for various machine learning algorithms was 0.835-1.000 in the training set, 0.786-0.949 in the testing set, and 0.689-0.818 in the external validation set. LR model had the highest AUC value, with 0.900 (95% CI: 0.852-0.947) in the training set, 0.901 (95% CI: 0.807-0.996) in the testing set, and 0.818 (95% CI: 0.714-0.923) in the external validation set. The atrophy pathological score based on the LR model was associated with endoscopic atrophy grading (Z=-2.478, P=0.013) and gastric cancer (GC) (OR=5.70, 95% CI: 2.63-12.33, P<0.001).

Conclusion

The ML model based on pathological features could improve the diagnostic consistency of gastric atrophy, which is also associated with endoscopic atrophy grading and GC.
Global research and emerging trends in autophagy in lung cancer: a bibliometric and visualized study from 2013 to 2022
Authors: Liu Bo-Na , Chen Juan , Piao Ying
Published: February 2024

Purpose: To highlight the knowledge structure and evolutionary trends in research on autophagy in lung cancer.

Methods: Research publications on autophagy in lung cancer were retrieved from the Web of Science Core Collection database. VOSviewer and CiteSpace data analysis software were used for the bibliometric and visualization analysis of countries, institutions, authors, journals, and keywords related to this field.

Results: From 2013 to 2022, research on autophagy in lung cancer developed rapidly, showing rising trends in annual publications and citations. China (1,986 papers; 48,913 citations), Shandong University (77 publications; 1,460 citations), and Wei Zhang (20 publications; 342 citations) were the most productive and influential country, institution, and author, respectively. The journal with the most publications and citations on autophagy in lung cancer was the International Journal of Molecular Sciences (93 publications; 3,948 citations). An analysis of keyword co-occurrence showed that related research topics were divided into five clusters: 1) Mechanisms influencing autophagy in lung cancer and the role of autophagy in lung cancer; 2) Effect of autophagy on the biological behavior of lung cancer; 3) Regulatory mechanisms of 2 cell death processes: autophagy and apoptosis in lung cancer cells; 4) Role of autophagy in lung cancer treatment and drug resistance; and 5) Role of autophagy-related genes in the occurrence and development of lung cancer. Cell proliferation, migration, epithelial-mesenchymal transition, and tumor microenvironment were the latest high-frequency keywords that represented promising future research directions.

Conclusion: This is the first comprehensive study describing the knowledge structure and emerging frontiers of research on autophagy in lung cancer from 2013 to 2022 by means of a bibliometric analysis. The study points to promising future research directions focusing on in-depth autophagy mechanisms, clinical applications, and potential therapeutic strategies, providing a valuable reference for researchers in the field.

Systematic Review Registration: [https://systematicreview.gov/], identifier [registration number].
A high-density linkage map construction in guava (Psidium guajava L.) using genotyping by sequencing and identification of QTLs for leaf, peel, and pulp color in an intervarietal mapping population
Authors: Mathiazhagan Malarvizhi , Elangovan Dayanandhi , Chinnaiyan Vasugi , Shivashankara Kodthalu Seetharamaiah , Sudhakar Rao Darisi Venkata , Ravishankar Kundapura Venkataramana
Published: February 2024

Psidium guajava L. is an important fruit crop in the tropical and subtropical regions of the world. The advanced breeding methods are not employed for important commercial traits like peel and pulp color, seed hardiness, fruit size, etc., due to the scarcity of genome-wide molecular markers and high-density linkage maps. In this study, we employed single-nucleotide polymorphism (SNP) markers and identified quantitative trait loci (QTL) regions that are associated with color traits of leaf, peel, and pulp in the guava intervarietal mapping population. The mapping population was developed from the contrasting genotypes of fruit and leaf color. Variations in color among the segregating hybrids were recorded both visually and using a Color reader. A high-density linkage map of guava was constructed using the SNP markers from genotyping by sequencing (GBS) of 150 hybrid individuals of the cross ‘Arka Poorna’ (green) x ‘Purple Local’ (purple). The integrated linkage map consisted of 1426 SNPs mapped on 11 linkage groups (LG), spanning a total distance of around 730 cM with an average of 129.6 markers per LG. Through QTL analysis for color traits, a minor QTL region was identified for visually scored leaf color and peel color on LG1, whereas a major QTL was detected for pulp color in LG4. The Hunter color values (L* and, a*) also had major QTLs with overlapping marker intervals for leaf and peel colors, establishing the association of SNP markers to the trait. The QTLs harbored genes and transcription factors involved in lycopene and anthocyanin pigment biosynthesis. This is the first report of a high-density linkage map based on SNP markers in guava and QTL mapping for color characters in leaf, fruit peel and pulp. The genotyping information generated in this study can aid in genetic engineering and marker-assisted breeding in guava.
Writing to your past-self can make you feel better
Authors: Sugimori Eriko , Yamaguchi Mayu , Kusumi Takashi
Published: February 2024

Self-compassionate writing has been shown to be helpful for improving the mental state in some individuals. Here, we investigated how the writer’s attitude toward his/her past, present and future and the focus of the writing, i.e., social experience in the past versus self-experience, modulate these effects. In Experiment 1, 150 undergraduates wrote a compassionate letter to their past-self and to their future-self and responded to the Japanese version of the Adolescent Time Inventory–Time Attitudes (ATI-TA) questionnaire. Writing to past-self decreased negative feelings more than writing to future-self. Further, participants who had negative feelings toward their past, present, and future, as assessed by the ATI-TA, were more likely to be emotionally affected by writing a letter to their past-self. In Experiment 2, 31 undergraduates wrote a letter focusing on what they had experienced together with someone, and another 31 undergraduates wrote focusing on what they had experienced alone. Focusing on a social experience was more helpful for recovering from negative feelings than focusing on a self-experience. In conclusion, writing a compassionate letter to one’s past-self can improve mood, especially in individuals with a negative time attitude who focus their writing on a social connection.
Virtual reality hypnosis prior to radiofrequency thermocoagulation for patients with chronic pain: an exploratory clinical trial
Authors: Safy Othmane , Rousseaux Floriane , Faymonville Marie-Elisabeth , Libbrecht Dominique , Fontaine Robert , Raaf Melissa , Staquet Cécile , Tasset Hadrien , Bonhomme Vincent , Vanhaudenhuyse Audrey , Bicego Aminata
Published: February 2024

Background

The management of chronic pain may involve an array of tools, including radiofrequency thermocoagulation (Rf-Tc) of sensory nerve terminals. Like many other invasive procedures, Rf-Tc can generate anxiety in a lot of patients, either during the expectation of the procedure or in the course of it. Virtual reality hypnosis (VRH) is a promising tool for managing anxiety and pain in several situations, but its anxiolytic property has not been investigated in participants with chronic pain and going through a Rf-Tc procedure.

Objectives

The goal of this study was to evaluate the effectiveness of VRH for reducing self-assessed anxiety in participants with chronic pain, when received in preparation for Rf-Tc.

Materials and methods

This prospective, controlled trial was conducted in the Interdisciplinary Algology Centre of the University Hospital of Liège (Belgium). Participants were assigned to two groups: VRH or control (usual care). Assessment was carried-out at 4 time points: T0 (one week before Rf-Tc); T1 (pre-intervention, on the day of Rf-Tc); T2 (immediately after the VRH intervention outside of the Rf-Tc room); and T3 (right after Rf-Tc). Medical, sociodemographic data, anxiety trait and immersive tendencies were collected at T0. Anxiety state and pain intensity were assessed at each time points. Satisfaction was examined at T3.

Results

Forty-two participants were quasi-randomly assigned to the VRH or control group. No statistically significant interaction group by time was observed regarding all measured variables, including primary endpoint. However, a significant effect of time was found for anxiety and pain when considering both groups together, toward a progressive reduction.

Conclusion

In the context of our study, there appears to be no significant effect of VRH at reducing anxiety in participants with chronic pain undergoing Rf-Tc. Anxiety decreases along the procedure, while pain is attenuated by the local anesthetic infiltration of the Rf site. Our results suggest that the presence of a caregiver throughout the procedure might explain the progressive decrease in anxiety. Future randomized controlled trials are needed to precisely study the effectiveness of the VRH tool, and the possibility of using it as a complementary approach for anxiety during invasive procedures.
The predictive validity of the V-RISK-10 and BVC among involuntarily admitted patients
Authors: Kös Tilmann , Bräunig Peter , Hausam Joscha
Published: February 2024

Although endangerment towards others is a criterion for an involuntary admission in many countries, research on risk assessment of endangerment among involuntarily admitted individuals is limited. In this retrospective case-control study, we calculated scores for a German-translated version of the Violence Risk Screening-10 (V-RISK-10) and the Brøset Violence Checklist (BVC) in a sample of 111 people undergoing an involuntary admission in Reinickendorf, Berlin. Outcomes were violence, coercive measures, and readmission. In line with our hypotheses, the BVC demonstrated stronger predictive validities for short-term, and V-RISK-10 for long-term events. There was an incremental validity for both instruments for restraint 24 hours after admission and any violence until discharge. These findings support the evidence that structured risk assessment instruments may be useful for individuals undergoing an involuntary admission. Ethical considerations about screening procedures are discussed.
Medical insurance, vulnerability to poverty, and wealth inequality
Authors: Zhou Xianhua , Yang Xujin
Published: February 2024

Background

China has made remarkable achievements in alleviating poverty under its current poverty standards. Despite these immense successes, the challenge of consolidating these achievements remains. In reality, health risks are among the significant factors causing rural households to fall into poverty, and medical insurance is the significant factor mitigating household vulnerability to poverty. Therefore, alleviating or guarding against households falling into poverty is essential.

Methods

This paper establishes a multi-equilibrium model that incorporates heterogeneous health risks and medical insurance. Through parameter calibration and value function iteration, numerical solutions are derived.

Results

Heterogeneous health risks significantly increase poverty vulnerability and wealth inequality in rural households. Medical insurance, through its investment incentives and loss compensation effects, efficiently mitigates these issues, especially benefiting those in poorer health. Furthermore, the dual-slanted compensation policy efficiently mitigates the adverse effects of “reverse redistribution.”

Conclusion

Medical insurance effectively mitigates household vulnerability to poverty and wealth inequality. Government departments must establish health records for residents. By recognizing variations in health conditions, these departments can provide households with poorer health conditions with a higher medical expense compensation ratio. In addition, the government should further focus medical expense reimbursements toward households on the cusp of escaping poverty to ensure that they are not plunged back (or further) into poverty due to medical expenses.
Factors related to depression in adults with oral health problems in Spain (2017 to 2020)
Authors: Cebrino Jesús , Portero de la Cruz Silvia
Published: February 2024

Background

The need to study the link between gender, depression, and oral health is becoming increasingly evident. This study therefore aimed to determine the prevalence and evolution over time of depression among women and men with oral health problems and to evaluate the association between depression status, lifestyle-related variables health-related variables and use of dental health services in those people.

Methods

We performed a nationwide cross-sectional study on 25,631 adults with oral health problems residing in Spain from the Spanish National Health Survey 2017 and the European Health Survey of Spain 2020, including as the main variable self-reported diagnosis of depression. We analysed independent variables such as lifestyle-related variables, health-related variables, and variables related to dental health services. Sociodemographic characteristics were considered as control variables.

Results

The prevalence of depression among adults with oral health problems in Spain was 7.81% (10.14% for women, 5.39% for men), with a notable decrease from 2017 to 2020 in women. Depressed women had a slightly higher percentage of filled or capped teeth, and had more covers (crowns), bridges or other types of prostheses or dentures, while men had more caries. Women also made more frequent, regular dental visits for check-ups and mouth cleaning, whereas men often needed extractions. Unfavourable associated factors in both genders were: perceiving their health as good, average, poor, or very poor, and having 1–2 and ≥ 3 comorbidities. Conversely, not being a current smoker was related to less likelihood of depression. In women only, not engaging in leisure-time physical activity produced more unfavourable associated factors.

Conclusion

The prevalence of depression among adults with oral health problems in Spain from 2017 to 2020 was 7.81%, but this figure has been steadily decreasing over time. In addition, the favourable and unfavourable associated factors could help us inform health professionals and authorities in order to prevent depression and enhance the care of this population according to gender.
Impact of normalized COVID-19 prevention and control measures on lower respiratory tract infection pathogenesis in hospitalized children
Authors: Feng Yuan , Zhang Huaixiao , Zhang Bo , Zhou Yinfei , Yuan Haibin
Published: February 2024

Objective

This study aimed to investigate the epidemiological characteristics of common pathogens contributing to childhood lower respiratory tract infections (LRTIs) in Xiangtan City, Hunan Province before and during the coronavirus disease 2019 (COVID-19) pandemic.

Methods

A total of 11,891 enrolled patients, aged 1 month to 14 years, diagnosed with LRTIs and admitted to Xiangtan Central Hospital from January 2018 to December 2021 were retrospectively reviewed in this study. Specifically, the epidemiological characteristics of these pathogens before and during the COVID-19 pandemic were analyzed.

Results

There was a significant decrease in the number of children hospitalized with LRTIs during the COVID-19 pandemic (2020–2021) compared to data from 2018 to 2019 (before the COVID-19 pandemic). Of these cases, 60.01% (7,136/11,891) were male and 39.99% (4,755/11,891) were female. 78.9% (9,381/11,891) cases occurred in children under 4 years of age. The average pathogen detection rate among 11,891 hospitalized LRTIs children was 62.19% (7,395/11,891), with the average pathogen detection rate of 60.33% (4,635/7,682) and 65.57% (2,670/4,209) before and during COVID-19 pandemic, respectively. The detection rates of adenovirus (ADV), bordetella pertussis (BP) and moraxella catarrhalis (M. catarrhalis) decreased dramatically, while the detection rates of influenza viruses (IFV), parainfluenza viruses (PIV), respiratory syncytial virus (RSV), haemophilus influenzae (H. influenzae), streptococcus pneumoniae (S. pneumoniae), and staphylococcus aureus (S. aureus) increased significantly during the COVID-19 pandemic. Overall, RSV, mycoplasma pneumoniae (MP), H. influenzae, and IFV were the major pathogens causing LRTIs in hospitalized children before and during the COVID-19 pandemic.

Conclusion

Public health interventions for COVID-19 prevention are beneficial to reduce the incidence of LRTIs in children by limiting the prevalence of ADV, MP, BP, and M. catarrhalis, but which have limited restrictive effects on other common LRTIs-associated pathogens. Collectively, the data in this study comprehensively investigated the effects of COVID-19 pandemic on the epidemiological characteristics of respiratory pathogens, which will be beneficial for improving early preventive measures.
Robotic systems for upper-limb rehabilitation in multiple sclerosis: a SWOT analysis and the synergies with virtual and augmented environments
Authors: Albanese Giulia A. , Bucchieri Anna , Podda Jessica , Tacchino Andrea , Buccelli Stefano , De Momi Elena , Laffranchi Matteo , Mannella Kailynn , Holmes Michael W. R. , Zenzeri Jacopo , De Michieli Lorenzo , Brichetto Giampaolo , Barresi Giacinto
Published: February 2024

The robotics discipline is exploring precise and versatile solutions for upper-limb rehabilitation in Multiple Sclerosis (MS). People with MS can greatly benefit from robotic systems to help combat the complexities of this disease, which can impair the ability to perform activities of daily living (ADLs). In order to present the potential and the limitations of smart mechatronic devices in the mentioned clinical domain, this review is structured to propose a concise SWOT (Strengths, Weaknesses, Opportunities, and Threats) Analysis of robotic rehabilitation in MS. Through the SWOT Analysis, a method mostly adopted in business management, this paper addresses both internal and external factors that can promote or hinder the adoption of upper-limb rehabilitation robots in MS. Subsequently, it discusses how the synergy with another category of interaction technologies - the systems underlying virtual and augmented environments - may empower Strengths, overcome Weaknesses, expand Opportunities, and handle Threats in rehabilitation robotics for MS. The impactful adaptability of these digital settings (extensively used in rehabilitation for MS, even to approach ADL-like tasks in safe simulated contexts) is the main reason for presenting this approach to face the critical issues of the aforementioned SWOT Analysis. This methodological proposal aims at paving the way for devising further synergistic strategies based on the integration of medical robotic devices with other promising technologies to help upper-limb functional recovery in MS.
Diversity of lipid profiles of Symbiodiniaceae under temperature and nutrient stress
Authors: La Motta Laura M. , Padula Matthew P. , Sommer Brigitte , Camp Emma F. , Matthews Jennifer L.
Published: February 2024

Lipid-based survival mechanisms allow microalgae to occupy wide geographical ranges and survive abiotic stress. The protist Symbiodiniaceae are globally distributed from temperate to tropical environments, and establish mutualisms with numerous hosts, including cnidarians. The ability for these dinoflagellates to maintain cellular function under wide ranging environmental conditions will influence the survival and geographic distribution of their hosts. One mechanism that microalgae utilize to adapt to environmental changes is lipid remodeling, such as increased saturation of membranes to maintain the structural integrity under temperature changes, and lipid accumulation when nutrient availability decreases. Whether Symbiodiniaceae utilize lipid remodeling to adapt to sublethal environmental change is yet to be resolved. This study examines the effects of temperature (16°C to 31°C), and nitrogen (N) and phosphorus (P) availability, on the lipid composition and physiology of cultured Symbiodiniaceae (from genera Breviolum, Cladocopium and Durusdinium) isolated from temperate or tropical environments. Glycerolipids, particularly triacyclglycerols, increased while cell size decreased under N- and NP-nutrient limited cultures, across all Symbiodiniaceae species. P-limitation caused a decrease in phosphatidylcholine, an important membrane lipid, and saw an increase in isoprenol lipids. This suggests a diversion of phosphorus from phospholipid membranes to the biosynthesis of membrane-stabilizing isoprenes. Reduced photophysiology under P-limitation in all Symbiodiniaceae further supports evidence that P-limitation induced stress in these Symbiodiniaceae cells. As expected, growth rate was reduced in all Symbiodiniaceae at temperature extremes (31°C). Significant increases in oxidized lipids, particularly oxidized phosphatidylinositol, and a reduction in ether-linked phospholipids in cultures grown at 31°C, suggests increased reactive oxygen species (ROS) abundance in these cells. In addition, at 31 °C, D. trenchii and both C. goreaui spp. cell size increased, a common sign of ROS accumulation, cell cycle arrest and necrosis. The observed increases in lipid energy storage (triacylglycerols and isoprenoids) under nutrient stress, as well as ROS-mitigation via lipid remodeling leading to increases in saturated fatty acids and oxidized lipids under temperatures stress, suggest Symbiodiniaceae can remodel their lipids to adapt to environmental shifts. If similar mechanisms occur in hospite, this could be an adaptive strategy for coral holobionts under a changing climate.
Effects of anomalous cosmic rays on the solar wind events in the outer heliosphere
Authors: Zhou Yucheng , Guo Xiaocheng , Wang Chi
Published: February 2024

Anomalous cosmic-rays (ACRs) are thought to be originated from the acceleration of pickup ions (PUIs) at the termination shock or interplanetary shocks, and play important role for the plasma dynamics in the outer heliosphere. Due to limited observation, the effects of ACRs on the solar wind events is not well known. Under the approximation of spherical symmetry, we have developed a three-component magneto-hydrodynamic (MHD) numerical model that contains solar wind plasma, interstellar neutral atoms and ACRs, to investigate the evolution of the solar wind within a heliocentric distance from 1 to 150 astronomical units (AU). We use the solar wind observations from the OMNI database with the time from 2010.5 to 2016.0 (decimal years) at the inner boundary, and the effect of ACRs on the propagation of the solar wind events are compared with the observations from the spacecrafts of New Horizons, Voyager 1 and 2. The results show that ACRs may decrease the speed of the solar wind shocks to some extent, and the effect is positively correlated with the diffusion coefficient; a larger diffusion coefficient leads to a more pronounced effect. Moreover, the ACRs has a dissipation effect on the shock-like solar wind structures, and may play important roles on the dynamics of solar wind in the outer heliosphere.
Gold Artifacts from the Early Scythian Princely Tomb Arzhan 2, Tuva—Aesthetics, Function, and Technology
Authors: Armbruster Barbara , Meyer Caspar
Published: February 2024

This article explores the extraordinarily rich gold finds from the Early Scythian princely tomb Arzhan 2 in the Republic of Tuva, southern Siberia (late 7th to early 6th centuries BCE), through the methodological framework of the chaîne opératoire (operational sequence), in order to reconstruct the objects’ processes of manufacture. Through an interdisciplinary study of the finds at the State Hermitage in Saint Petersburg, the principal author analyzed tool marks and surface morphologies, which allow for the comprehensive identification and documentation of the numerous techniques employed in the creation of the often very elaborate jewelry, decorated weapons, and other personal ornaments. The production of both individual pieces and extensive series of thousands of identical trimmings attests to the existence of complex craft processes and workshop organizations. The technological aspects of the gold finds impress through their diversity and outstanding quality, both artistically and in terms of their craftsmanship. As this article will demonstrate, the objects present the earliest evidence for a highly specialized goldsmith artform in southern Siberia.
Multidisciplinary Team Care in Pituitary Tumours
Authors: Marques Pedro , Sagarribay Amets , Tortosa Francisco , Neto Lia , Tavares Ferreira Joana , Subtil João , Palha Ana , Dias Daniela , Sapinho Inês
Published: February 2024

The optimal care for patients with pituitary tumours is best provided in a multidisciplinary and collaborative environment, which requires the contribution of multiple medical specialties working together. The benefits and advantages of the pituitary multidisciplinary team (MDT) are broad, and all relevant international consensus and guidelines in the field recommend that patients with pituitary tumours should always be managed in a MDT. Endocrinologists and neurosurgeons are normally the leading specialties within the pituitary MDT, supported by many other specialties with significant contributions to the diagnosis and management of pituitary tumours, including neuropathology, neuroradiology, neuro-ophthalmology, and otorhinolaryngology, among others. Here, we review the literature concerning the concepts of Pituitary MDT/Pituitary Tumour Centre of Excellence (PTCOE) in terms of their mission, goals, benefits, structure, proposed models of function, and barriers, and we also provide the views of different specialists involved in our Pituitary MDT.
A Systematic Review of Agroecology Strategies for Adapting to Climate Change Impacts on Smallholder Crop Farmers’ Livelihoods in South Africa
Authors: Zenda Mashford , Rudolph Michael
Published: February 2024

This systematic review identified the prevalence, effectiveness, and potential benefits of agroecology strategies in promoting sustainable agriculture practices implemented by smallholder crop farmers in South Africa. The review carried out a comprehensive literature search across various academic databases, including PubMed, Scopus, and Web of science. The relevant studies were screened and selected based on predetermined inclusion criteria where a total of 262 articles were extracted and reduced to 30 articles for this systematic review. Data were extracted and synthesised to classify patterns and trends in the adoption of agroecology elements. The results obtained from the review of this study highlights the identification of specific strategies such as indigenous crop varieties, conservation agriculture, intercropping, agroforestry, drought-tolerant crop varieties, and water management strategies. These outcomes demonstrated insights into the prevalence of different strategies applied by smallholder crop farmers in South Africa. Furthermore, the review determined the reported benefits, such as increased crop resilience, improved soil fertility, and enhanced water use efficiency. These benefits were assessed on the available evidence from the selected studies. This review contributes to a better understanding of agroecology practices in South African. The results can inform policymakers, researchers, and farmers in developing appropriate strategies to enhance sustainable agricultural practices.
Pairwise CNN-Transformer Features for Human–Object Interaction Detection
Authors: Quan Hutuo , Lai Huicheng , Gao Guxue , Ma Jun , Li Junkai , Chen Dongji
Published: February 2024

Human–object interaction (HOI) detection aims to localize and recognize the relationship between humans and objects, which helps computers understand high-level semantics. In HOI detection, two-stage and one-stage methods have distinct advantages and disadvantages. The two-stage methods can obtain high-quality human–object pair features based on object detection but lack contextual information. The one-stage transformer-based methods can model good global features but cannot benefit from object detection. The ideal model should have the advantages of both methods. Therefore, we propose the Pairwise Convolutional neural network (CNN)-Transformer (PCT), a simple and effective two-stage method. The model both fully utilizes the object detector and has rich contextual information. Specifically, we obtain pairwise CNN features from the CNN backbone. These features are fused with pairwise transformer features to enhance the pairwise representations. The enhanced representations are superior to using CNN and transformer features individually. In addition, the global features of the transformer provide valuable contextual cues. We fairly compare the performance of pairwise CNN and pairwise transformer features in HOI detection. The experimental results show that the previously neglected CNN features still have a significant edge. Compared to state-of-the-art methods, our model achieves competitive results on the HICO-DET and V-COCO datasets.
Research on Risk Contagion in ESG Industries: An Information Entropy-Based Network Approach
Authors: Hu Chenglong , Guo Ranran
Published: February 2024

Sustainable development is a practical path to optimize industrial structures and enhance investment efficiency. Investigating risk contagion within ESG industries is a crucial step towards reducing systemic risks and fostering the green evolution of the economy. This research constructs ESG industry indices, taking into account the possibility of extreme tail risks, and employs VaR and CoVaR as measures of tail risk. The TENET network approach is integrated to to capture the structural evolution and direction of information flow among ESG industries, employing information entropy to quantify the topological characteristics of the network model, exploring the risk transmission paths and evolution patterns of ESG industries in an extreme tail risk event. Finally, Mantel tests are conducted to examine the existence of significant risk spillover effects between ESG and traditional industries. The research finds strong correlations among ESG industry indices during stock market crash, Sino–US trade frictions, and the COVID-19 pandemic, with industries such as the COAL, CMP, COM, RT, and RE playing key roles in risk transmission within the network, transmitting risks to other industries. Affected by systemic risk, the information entropy of the TENET network significantly decreases, reducing market information uncertainty and leading market participants to adopt more uniform investment strategies, thus diminishing the diversity of market behaviors. ESG industries show resilience in the face of extreme risks, demonstrating a lack of significant risk contagion with traditional industries.
A New Approach to Use of Traction Power Network in Poland for Charging Electric Vehicles
Authors: Mazur Łukasz , Bieliński Kazimierz Stanisław , Kłosowski Zbigniew
Published: February 2024

Electric vehicles are increasingly appearing on Polish roads due to a number of technical, legal and marketing conditions. However, electromobility is developing primarily in urban areas, mainly due to the unevenly developed infrastructure for charging vehicle batteries and the power grid. Therefore, solutions should be created that use the existing power infrastructure, including the use of railway power infrastructure (RPI). The railway power network covers a significant part of the country, including forest areas, and, above all, it very often intersects with road infrastructure or runs along roads. This paper raises issues related to the possibility of using RPI to charge the batteries of electric vehicles. After characterizing the technical, operational and legal requirements related to these technical systems, a concept of an electric vehicle charging system using RPI was developed, along with a demonstration of the possibility of its implementation, which was simulated using mathematical models developed by the authors.
Unraveling the Role of RNase L Knockout in Alleviating Immune Response Activation in Mice Bone Marrow after Irradiation
Authors: Ding Kexin , Li Hujie , Tai Fumin , Duan Junzhao , Wang Qiong , Zhai Rui , Fu Hanjiang , Ge Changhui , Zheng Xiaofei
Published: February 2024

Ionizing radiation (IR) induces severe hematopoietic injury by causing DNA and RNA damage as well as activating the immune responses, necessitating the development of effective therapeutic strategies. Ribonuclease L (RNase L) as an innate immune response pathway is triggered by exogenous and endogenous abnormal dsRNA under viral infection and dyshomeostasis, thereby activating the immune responses. Thus, we investigated the effect of RNase L on irradiation-induced bone marrow damage using RNase L knockout (RNase L−/−) mice. Phenotypic analysis revealed that RNase L knockout mitigates irradiation-induced injury in the bone marrow. Further investigation into the mechanism of RNase L by RNA-seq, qRT-PCR, and CBA analysis demonstrated that RNase L deficiency counteracts the upregulation of genes related to immune responses induced by irradiation, including cytokines and interferon-stimulated genes. Moreover, RNase L deficiency inhibits the increased levels of immunoglobulins in serum induced by irradiation. These findings indicate that RNase L plays a role in the immune response induced by irradiation in the bone marrow. This study further enhances our understanding of the biological functions of RNase L in the immune response induced by irradiation and offers a novel approach for managing irradiation-induced bone marrow injury through the regulation of RNase L activation.
Genome-Wide Analysis of the Liriodendron chinense Hsf Gene Family under Abiotic Stress and Characterization of the LcHsfA2a Gene
Authors: Yang Yun , Yin Jianchao , Zhu Liming , Xu Lin , Wu Weihuang , Lu Ye , Chen Jinhui , Shi Jisen , Hao Zhaodong
Published: February 2024

Heat shock factors (Hsfs) play a crucial role in plant defense processes. However, the distribution and functional characteristics of Hsf genes in the relict plant Liriodendron chinense are still unclear. In this study, a total of 19 LcHsfs were identified and divided into three separate subgroups, comprising 10 LcHsfA, 7 LcHsfB, and 2 LcHsfC genes, respectively, based on their phylogenetic tree and the presence/absence of conserved protein domains. Whole-genome duplication and segmental duplication led to an expansion of the LhHsf gene family. The promoters of LcHsf genes are enriched for different types of cis-acting elements, including hormone responsive and abiotic-stress-responsive elements. The expression of LcHsfA3, LcHsfA4b, LcHsfA5, LcHsfB1b, and LcHsfB2b increased significantly as a result of both cold and drought treatments. LcHsfA2a, LcHsfA2b, and LcHsfA7 act as important genes whose expression levels correlate strongly with the expression of the LcHsp70, LcHsp110, and LcAPX genes under heat stress. In addition, we found that transiently transformed 35S:LcHsfA2a seedlings showed significantly lower levels of hydrogen peroxide (H2O2) after heat stress and showed a stronger thermotolerance. This study sheds light on the possible functions of LcHsf genes under abiotic stress and identifies potentially useful genes to target for molecular breeding, in order to develop more stress-resistant varieties.
The Upregulation of Leucine-Rich Repeat Containing 1 Expression Activates Hepatic Stellate Cells and Promotes Liver Fibrosis by Stabilizing Phosphorylated Smad2/3
Authors: Wang Yake , Li Xiaolong , Guan Xiaowen , Song Zhe , Liu Huanfei , Guan Zhenzhen , Wang Jianwei , Zhu Lina , Zhang Di , Zhao Liang , Xie Peitong , Wei Xiaoyi , Shang Ning , Liu Ying , Jin Zhongzhen , Ji Zhili , Dai Guifu
Published: February 2024

Liver fibrosis poses a significant global health risk due to its association with hepatocellular carcinoma (HCC) and the lack of effective treatments. Thus, the need to discover additional novel therapeutic targets to attenuate liver diseases is urgent. Leucine-rich repeat containing 1 (LRRC1) reportedly promotes HCC development. Previously, we found that LRRC1 was significantly upregulated in rat fibrotic liver according to the transcriptome sequencing data. Herein, in the current work, we aimed to explore the role of LRRC1 in liver fibrosis and the underlying mechanisms involved. LRRC1 expression was positively correlated with liver fibrosis severity and significantly elevated in both human and murine fibrotic liver tissues. LRRC1 knockdown or overexpression inhibited or enhanced the proliferation, migration, and expression of fibrogenic genes in the human hepatic stellate cell line LX-2. More importantly, LRRC1 inhibition in vivo significantly alleviated CCl4-induced liver fibrosis by reducing collagen accumulation and hepatic stellate cells’ (HSCs) activation in mice. Mechanistically, LRRC1 promoted HSC activation and liver fibrogenesis by preventing the ubiquitin-mediated degradation of phosphorylated mothers against decapentaplegic homolog (Smad) 2/3 (p-Smad2/3), thereby activating the TGF-β1/Smad pathway. Collectively, these results clarify a novel role for LRRC1 as a regulator of liver fibrosis and indicate that LRRC1 is a promising target for antifibrotic therapies.