bluuebunny/crossref_metadata_2025 huggingface dataset:
Effects of Copper Slag as a Replacement for Fine Aggregate on the Behavior and Ultimate Strength of Reinforced Concrete Slender Columns
Authors: Alnuaimi AS
Published: June 2017

Use of copper slag (CS) as a replacement for fine aggregate (FA) in RC slender columns was experimentally investigated in this study. Twenty columns measuring 150 mm x150 mm x 2500 mm were tested for monotonic axial compression load until failure. The concrete mixture included ordinary Portland cement (OPC) cement, fine aggregate, 10 mm coarse aggregate, and CS. The cpercentage of cement, water and coarse aggregate were kept constant within the mixture, while the percentage of CS as a replacement for fine aggregate varied from 0 to 100%. Four 8 mm diameter high yield steel and 6 mm mild steel bars were used as longitudinal and transverse reinforcement, respectively. Five cubes measuring 100 mm x100 mm x100 mm, eight cylinders measuring 150 mm x 300 mm and five prisms measuring 100 mm x 100 mm x 500 mm were cast and tested for each mixture to determine the compressive and tensile strengths of the concrete. The results showed that the replacement of up to 40% of the fine aggregate with CS caused no major changes in concrete strength, column failure load, or measured flexural stiffness (EI). Further increasing the percentage reduced the concrete strength, column failure load, and flexural stiffness (EI), and increased concrete slump and lateral and vertical deflections of the column. The maximum difference in concrete strength between the mixes of 0% CS and 100% CS was 29%, with the difference between the measured/ control failure loads between the columns with 0 and 100% CS was 20% the maximum difference in the measured EI between the columns with 0 and 100% CS was 25%. The measured to calculated failure loads of all specimen varied between 91 and -100.02%. The measured steel strains were proportional to the failure loads. It was noted that columns with high percentages of CS (
Nonlinear Model Predictive Control for Unmanned Aerial Vehicles
Authors: Ru Pengkai , Subbarao Kamesh
Published: June 2017

This paper discusses the derivation and implementation of a nonlinear model predictive control law for tracking reference trajectories and constrained control of a quadrotor platform. The approach uses the state-dependent coefficient form to capture the system nonlinearities into a pseudo-linear system matrix. The state-dependent coefficient form is derived following a rigorous analysis of aerial vehicle dynamics that systematically accounts for the peculiarities of such systems. The same state-dependent coefficient form is exploited for obtaining a nonlinear equivalent of the model predictive control. The nonlinear model predictive control law is derived by first transforming the continuous system into a sampled-data form and and then using a sequential quadratic programming solver while accounting for input, output and state constraints. The boundedness of the tracking errors using the sampled-data implementation is shown explicitly. The performance of the nonlinear controller is illustrated through representative simulations showing the tracking of several aggressive reference trajectories with and without disturbances.
Zirconium Phosphate Catalysts in the XXI Century: State of the Art from 2010 to Date
Authors: Pica Monica
Published: June 2017

An overview on the developments of zirconium phosphate (ZrP) and its organic derivatives in heterogeneous catalysis in recent years is reported in the present review. Two basic aspects have been emphasized: first, the catalytic properties of zirconium phosphates were discussed, with particular attention to the effect of surface acidity and hydrophobic/hydrophilic character, textural properties, and particle morphology on the catalytic performances. Then, the use of zirconium phosphates as support for catalytic active species was reported, including organometallic complexes, metal ions, noble metal, and metal oxide nanoparticles. Zirconium phosphate plays, in those cases, a dual role, since it promotes the dispersion and stabilization of the catalysts, thanks to their interaction with the active sites on the surface of ZrP, and facilitates the recovery and reuse of the catalytic species due to their immobilization on the solid support.
Telomere Biology—Insights into an Intriguing Phenomenon
Authors: Venkatesan Shriram , Khaw Aik , Hande Manoor
Published: June 2017

Bacteria and viruses possess circular DNA, whereas eukaryotes with typically very large DNA molecules have had to evolve into linear chromosomes to circumvent the problem of supercoiling circular DNA of that size. Consequently, such organisms possess telomeres to cap chromosome ends. Telomeres are essentially tandem repeats of any DNA sequence that are present at the ends of chromosomes. Their biology has been an enigmatic one, involving various molecules interacting dynamically in an evolutionarily well-trimmed fashion. Telomeres range from canonical hexameric repeats in most eukaryotes to unimaginably random retrotransposons, which attach to chromosome ends and reverse-transcribe to DNA in some plants and insects. Telomeres invariably associate with specialised protein complexes that envelop it, also regulating access of the ends to legitimate enzymes involved in telomere metabolism. They also transcribe into repetitive RNA which also seems to be playing significant roles in telomere maintenance. Telomeres thus form the intersection of DNA, protein, and RNA molecules acting in concert to maintain chromosome integrity. Telomere biology is emerging to appear ever more complex than previously envisaged, with the continual discovery of more molecules and interplays at the telomeres. This review also includes a section dedicated to the history of telomere biology, and intends to target the scientific audience new to the field by rendering an understanding of the phenomenon of chromosome end protection at large, with more emphasis on the biology of human telomeres. The review provides an update on the field and mentions the questions that need to be addressed.
Aluminizing via Ionic Liquid Electrodeposition and Pack Cementation: A Comparative Study with Inconel 738 and a CoNiCrAlY
Authors: Tagliaferri Luca , Berretti Enrico , Giaccherini Andrea , Martinuzzi Stefano M. , Bozza Francesco , Thoma Martin , Bardi Ugo , Caporali Stefano
Published: June 2017

A novel aluminizing process based upon room temperature Al-electrodeposition from Ionic Liquids followed by diffusion heat treatment was applied on bare- and CoNiCrAlY-coated Inconel 738 (IN738). The aluminized samples were tested by isothermal oxidation at 1000 °C in air. The microstructural and chemical evolution of the samples were determined as function of oxidation time and compared with the currently applied coatings obtained via pack cementation. The newly proposed method is suitable for the CoNiCrAlY coating, but not for the bare IN738. In the latter, the formed Al-enriched layer is much thinner and the anticorrosion properties resulted in being reduced. This is probably due to the presence of precipitates, which slow down the aluminum inward diffusion impairing the formation of a well-developed interdiffusion zone (IDZ). Traces of the electrolyte, embedded during the Al-electrodeposition process, can be seen as the origin of these precipitates.
Time-Correlated Vortex Tunneling in Layered Superconductors
Authors: Miller John , Villagrán Martha
Published: June 2017

The nucleation and dynamics of Josephson and Abrikosov vortices determine the critical currents of layered high-Tc superconducting (HTS) thin films, grain boundaries, and coated conductors, so understanding their mechanisms is of crucial importance. Here, we treat pair creation of Josephson and Abrikosov vortices in layered superconductors as a secondary Josephson effect. Each full vortex is viewed as a composite fluid of micro-vortices, such as pancake vortices, which tunnel coherently via a tunneling matrix element. We introduce a two-terminal magnetic (Weber) blockade effect that blocks tunneling when the applied current is below a threshold value. We simulate vortex tunneling as a dynamic, time-correlated process when the current is above threshold. The model shows nearly precise agreement with voltage-current (V-I) characteristics of HTS cuprate grain boundary junctions, which become more concave rounded as temperature decreases, and also explains the piecewise linear V-I behavior observed in iron-pnictide bicrystal junctions and other HTS devices. When applied to either Abrikosov or Josephson pair creation, the model explains a plateau seen in plots of critical current vs. thickness of HTS-coated conductors. The observed correlation between theory and experiment strongly supports the proposed quantum picture of vortex nucleation and dynamics in layered superconductors.
Heteroepitaxy, an Amazing Contribution of Crystal Growth to the World of Optics and Electronics
Authors: Tassev Vladimir
Published: June 2017

Advances in Electronics and Optics are often preceded by discoveries in Crystal Growth theory and practice. This article represents in retrospect some of the most significant contributions of heteroepitaxy in these and some other areas—the strong impact of the three modes of heteroepitaxy on microelectronics and quantum optics, the big “push” of PENDEO epitaxy in development of Light Emitting Diodes, etc. A large part of the text is dedicated to heteroepitaxy of nonlinear optical materials grown on orientation-patterned templates and used in the development of new quasi-phase-matching frequency conversion laser sources. By achieving new frequency ranges such sources will result in a wide variety of applications in areas such as defense, security, industry, medicine, and science. Interesting facts from the scientific life of major contributors in the field are mixed in the text with fine details from growth experiments, chemical equations, results from material characterizations and some optical and crystallographic considerations—all these presented in a popular way but without neglecting their scientific importance and depth. The truth is that often heteroepitaxy is not just the better but the only available option. The truth is that delays in device development are usually due to gaps in materials research. In all this, miscommunication between different scientific communities always costs vain efforts, uncertainty, and years of going in a wrong scientific direction. With this article we aim to stimulate a constructive dialog that could lead to solutions of important interdisciplinary scientific and technical issues.
LSTM-CRF for Drug-Named Entity Recognition
Authors: Zeng Donghuo , Sun Chengjie , Lin Lei , Liu Bingquan
Published: June 2017

Drug-Named Entity Recognition (DNER) for biomedical literature is a fundamental facilitator of Information Extraction. For this reason, the DDIExtraction2011 (DDI2011) and DDIExtraction2013 (DDI2013) challenge introduced one task aiming at recognition of drug names. State-of-the-art DNER approaches heavily rely on hand-engineered features and domain-specific knowledge which are difficult to collect and define. Therefore, we offer an automatic exploring words and characters level features approach: a recurrent neural network using bidirectional long short-term memory (LSTM) with Conditional Random Fields decoding (LSTM-CRF). Two kinds of word representations are used in this work: word embedding, which is trained from a large amount of text, and character-based representation, which can capture orthographic feature of words. Experimental results on the DDI2011 and DDI2013 dataset show the effect of the proposed LSTM-CRF method. Our method outperforms the best system in the DDI2013 challenge.
Multiscale Entropy Analysis of Unattended Oximetric Recordings to Assist in the Screening of Paediatric Sleep Apnoea at Home
Authors: Crespo Andrea , Álvarez Daniel , Gutiérrez-Tobal Gonzalo C. , Vaquerizo-Villar Fernando , Barroso-García Verónica , Alonso-Álvarez María L. , Terán-Santos Joaquín , Hornero Roberto , Campo Félix del
Published: June 2017

Untreated paediatric obstructive sleep apnoea syndrome (OSAS) can severely affect the development and quality of life of children. In-hospital polysomnography (PSG) is the gold standard for a definitive diagnosis though it is relatively unavailable and particularly intrusive. Nocturnal portable oximetry has emerged as a reliable technique for OSAS screening. Nevertheless, additional evidences are demanded. Our study is aimed at assessing the usefulness of multiscale entropy (MSE) to characterise oximetric recordings. We hypothesise that MSE could provide relevant information of blood oxygen saturation (SpO2) dynamics in the detection of childhood OSAS. In order to achieve this goal, a dataset composed of unattended SpO2 recordings from 50 children showing clinical suspicion of OSAS was analysed. SpO2 was parameterised by means of MSE and conventional oximetric indices. An optimum feature subset composed of five MSE-derived features and four conventional clinical indices were obtained using automated bidirectional stepwise feature selection. Logistic regression (LR) was used for classification. Our optimum LR model reached 83.5% accuracy (84.5% sensitivity and 83.0% specificity). Our results suggest that MSE provides relevant information from oximetry that is complementary to conventional approaches. Therefore, MSE may be useful to improve the diagnostic ability of unattended oximetry as a simplified screening test for childhood OSAS.
Large-Eddy Simulations of Two In-Line Turbines in a Wind Tunnel with Different Inflow Conditions
Authors: Ciri Umberto , Petrolo Giovandomenico , Salvetti Maria , Leonardi Stefano
Published: June 2017

Numerical simulations reproducing a wind tunnel experiment on two in-line wind turbines have been performed. The flow features and the array performances have been evaluated in different inflow conditions. Following the experimental setup, different inlet conditions are obtained by simulating two grids upstream of the array. The increased turbulence intensity due to the grids improves the wake recovery and the efficiency of the second turbine. However, the inlet grid induces off-design operation on the first turbine, decreasing the efficiency and increasing fatigue loads. Typical grid flow patterns are observed past the rotor of the first turbine, up to the near wake. Further downstream, the signature of the grid on the flow is quite limited. An assessment of numerical modeling aspects (subgrid scale tensor and rotor parameterization) has been performed by comparison with the experimental measurements.
Multi-Frequency Control in a Stand-Alone Multi-Microgrid System Using a Back-To-Back Converter
Authors: Yoo Hyeong-Jun , Nguyen Thai-Thanh , Kim Hak-Man
Published: June 2017

A stand-alone multi-microgrid (MMG) system can be formed by connecting multiple stand-alone microgrids (MGs). In the stand-alone MMG system where the frequencies of each MG system are different, a back-to-back (BTB) converter can be used for interconnecting the adjacent MG system. The frequency control performance of the MMG system can be improved by designing the suitable controller of the BTB converter. This study proposes a multi-frequency control in the BTB converter to improve the performance of frequency regulation in the MMG system. Autonomous power sharing between each MG system is achieved by using the proposed multi-frequency control. The stand-alone MMG system where two stand-alone MG systems with different nominal frequencies are interconnected using the BTB converter is simulated in this study to show the feasibility of the proposed multi-frequency controller. Each stand-alone MG system consists of an inverter-based distributed generator (DG) that uses a grid-forming converter with a conventional frequency droop controller. The inverter-based DG is responsible for the primary frequency control in each MG system. To show the effectiveness of the proposed multi-frequency control, a comparison study of the multi-frequency control and the single frequency control is presented in this study. Simulation results show that the system stability can be improved by using the proposed multi-frequency controller.
CFD Analysis on the Air-Side Thermal-Hydraulic Performance of Multi-Louvered Fin Heat Exchangers at Low Reynolds Numbers
Authors: Saleem Arslan , Kim Man-Hoe
Published: June 2017

The air-side thermal-hydraulic performance of multi-louvered aluminium fin heat exchangers is investigated. A systematic numerical study has been performed to analyze the air-sde thermal hydraulic characteristics over a wide range of Reynolds number i.e., from 30 to 500. Air-side heat transfer coefficient and pressure drop were calculated and validated over the mentioned band of Reynolds numbers. The critical Reynolds number was determined numerically; and also the variation of flow pattern along with the air-side heat transfer coefficient and pressure drop in a multi-louvered heat exchanger associated with R e c r i has been reported. Moreover, a parametric study of the multi-louvered aluminium fin heat exchangers was also performed for 36 heat exchanger configurations with the louver angles (19–31°); fin pitches (1.0, 1.2, 1.4 mm) and flow depths (16, 20, 24 mm); and the geometric configuration exhibiting the highest air-side heat transfer coefficient was reported. The air-side heat transfer coefficient and pressure drop results for different geometrical configurations were presented in terms of Colburn j factor and Fanning friction factor f; as a function of Reynolds number based on louver pitch.
Editorial Special Issue “Combustion and Propulsion”
Authors: De Giorgi Maria , Ficarella Antonio
Published: June 2017

The increasing demand for socially and environmentally sustainable development requires appropriate use of energy resources, particularly in the transportation of people and goods.[...]
Flexibility-Based Evaluation of Variable Generation Acceptability in Korean Power System
Authors: Min Chang-Gi , Kim Mun-Kyeom
Published: June 2017

This study proposes an evaluation method for variable generation (VG) acceptability with an adequate level of power system flexibility. In this method, a risk index referred to as the ramping capability shortage expectation (RSE) is used to quantify flexibility. The RSE value of the current power system is selected as the adequate level of flexibility (i.e., RSE criterion). VG acceptability is represented by the VG penetration level for the RSE criterion. The proposed evaluation method was applied to the generation expansion plan in Korea for 2029 in order to examine the validity of the existing plan for VG penetration. Sensitivity analysis was also performed to analyze the effects of changes in system uncertainty on VG acceptability. The results show that the planned VG penetration level for 2029 can improve by approximately 12% while securing flexibility.
Influence of Altitude on Biochemical Properties of European Beech (Fagus sylvatica L.) Forest Soils
Authors: De Feudis Mauro , Cardelli Valeria , Massaccesi Luisa , Lagomarsino Alessandra , Fornasier Flavio , Westphalen Danielle , Cocco Stefania , Corti Giuseppe , Agnelli Alberto
Published: June 2017

Climate warming is predicted to raise the mean global temperature by 1 °C in the next 50 years, and this change is believed to be capable of affecting soil organic matter cycling and nutrient availability. With the aim of increasing knowledge on the response of forest soils to the ongoing climate change, we used altitude as a proxy for temperature change and studied chemical and biochemical properties of European beech (Fagus sylvatica L.) forest soils at two altitudes (800 and 1000 m) from central Apennines (Italy). Results showed that 1 °C of mean annual air temperature difference between the sites at the two altitudes had greater effect on the mineral horizons than on the organic horizons. At higher altitude, mineral soil had limited development, higher pH, and higher organic matter content due to the lower efficiency of the microbial community. Enzymatic activities of the organic horizons were generally not affected by altitude. Conversely, we observed a higher activity of xylosidase, β-glucosidase, alkaline phosphomonoesterase, arylsulfatase, and leucine-aminopeptidase in the sub-superficial horizons (Bw1 and Bw2) of the soils at 1000 m. We hypothesized that, as a response to environmental and climatic constraints occurring at higher altitude, plant roots increase the production of enzymes directly and/or indirectly by triggering the microbial community through exudation.
Comparing Airborne Laser Scanning, and Image-Based Point Clouds by Semi-Global Matching and Enhanced Automatic Terrain Extraction to Estimate Forest Timber Volume
Authors: Ullah Sami , Dees Matthias , Datta Pawan , Adler Petra , Koch Barbara
Published: June 2017

Information pertaining to forest timber volume is crucial for sustainable forest management. Remotely-sensed data have been incorporated into operational forest inventories to serve the need for ever more diverse and detailed forest statistics and to produce spatially explicit data products. In this study, data derived from airborne laser scanning and image-based point clouds were compared using three volume estimation methods to aid wall-to-wall mapping of forest timber volume. Estimates of forest height and tree density metrics derived from remotely-sensed data are used as explanatory variables, and forest timber volumes based on sample field plots are used as response variables. When compared to data derived from image-based point clouds, airborne laser scanning produced slightly more accurate estimates of timber volume, with a root mean square error (RMSE) of 26.3% using multiple linear regression. In comparison, RMSEs for volume estimates derived from image-based point clouds were 28.3% and 29.0%, respectively, using Semi-Global Matching and enhanced Automatic Terrain Extraction methods. Multiple linear regression was the best-performing parameter estimation method when compared to k-Nearest Neighbour and Support Vector Machine. In many countries, aerial imagery is acquired and updated on regular cycles of 1–5 years when compared to more costly, once-off airborne laser scanning surveys. This study demonstrates point clouds generated from such aerial imagery can be used to enhance the estimation of forest parameters at a stand and forest compartment level-scale using small area estimation methods while at the same time achieving sampling error reduction and improving accuracy at the forest enterprise-level scale.
Attribution of Disturbance Agents to Forest Change Using a Landsat Time Series in Tropical Seasonal Forests in the Bago Mountains, Myanmar
Authors: Shimizu Katsuto , Ahmed Oumer S. , Ponce-Hernandez Raul , Ota Tetsuji , Win Zar Chi , Mizoue Nobuya , Yoshida Shigejiro
Published: June 2017

In 2016, in response to forest loss, the Myanmar government banned logging operations for 1 year throughout the entire country and for 10 years in specific regions. However, it is unclear whether this measure will effectively reduce forest loss, because disturbance agents other than logging may have substantial effects on forest loss. In this study, we investigated an approach to attribute disturbance agents to forest loss, and we characterized the attribution of disturbance agents, as well as the areas affected by these agents, in tropical seasonal forests in the Bago Mountains, Myanmar. A trajectory-based analysis using a Landsat time series was performed to detect change pixels. After the aggregation process that grouped adjacent change pixels in the same year as patches, a change attribution was implemented using the spectral, geometric, and topographic information of each patch via random forest modeling. The attributed agents of change include “logging”, “plantation”, “shifting cultivation”, “urban expansion”, “water invasion”, “recovery”, “other change”, and “no change”. The overall accuracy of the attribution model at the patch and area levels was 84.7% and 96.0%, respectively. The estimated disturbance area from the attribution model accounted for 10.0% of the study area. The largest disturbance agent was found to be logging (59.8%), followed by water invasion (14.6%). This approach quantifies disturbance agents at both spatial and temporal scales in tropical seasonal forests, where limited information is available for forest management, thereby providing crucial information for assessing forest conditions in such environments.
Stroke in Older Survivors of Ischemic Stroke: Standard Care or Something Different?
Authors: Hubbard Isobel , Wass Suzanne , Pepper Elizabeth
Published: June 2017

Stroke is one of the leading causes of death and disability and it is more likely to occur in those who are older. Because people are living longer, the definition of “old” continues to evolve. Age alone should not influence the healthcare that a patient receives, however, evidence indicates that this does occur, especially in older patients. On the basis of the available evidence, it is time to reconsider whether or not stroke care should differ in older survivors of stroke and if so, why. This is a narrative review of stroke-related health care in those with a recent ischemic stroke. It seeks to answer the following question: Should patients aged ≥80 years who have experienced a recent ischemic stroke receive standard care or something different, and if they should receive something different, what should they receive and why? The review focusses on long-term survival, hyper-acute care, secondary prevention, and rehabilitation. The authors propose a number of recommendations in relation to stroke care in older survivors of a recent ischemic stroke.
Lower Physical Performance in Colder Seasons and Colder Houses: Evidence from a Field Study on Older People Living in the Community
Authors: Hayashi Yukie , Schmidt Steven , Malmgren Fänge Agneta , Hoshi Tanji , Ikaga Toshiharu
Published: June 2017

The aim of this paper was to explore the effect of seasonal temperature differences and cold indoor environment in winter on the physical performance of older people living in the community based on a field study. We recruited 162 home-dwelling older people from a rehabilitation facility in the Osaka prefecture, Japan; physical performance data were available from 98/162 (60.5%). At the same time, for some participants, a questionnaire survey and a measurement of the indoor temperature of individual houses were conducted. The analysis showed that there were seasonal trends in the physical performance of older people and that physical performance was worse in the winter compared with the autumn. Furthermore, people living in colder houses had worse physical performance. The findings indicate that keeping the house warm in the winter can help to maintain physical performance.
Association between Six CETP Polymorphisms and Metabolic Syndrome in Uyghur Adults from Xinjiang, China
Authors: Hou Huixian , Ma Rulin , Guo Heng , He Jia , Hu Yunhua , Mu Lati , Yan Yizhong , Ma Jiaolong , Li Shugang , Zhang Jingyu , Ding Yusong , Zhang Mei , Niu Qiang , Liu Jiaming , Guo Shuxia
Published: June 2017

Objective: To explore the association between CETP gene polymorphisms and metabolic syndrome (MS), as well as the relationship between the CETP gene polymorphisms and each component of MS. Methods: A total of 571 individuals which were randomly selected from 5692 Uyghur adults were subdivided into two groups, including 280 patients with MS and 291 control subjects, using the group-matching method after matching for gender. We detected CETP polymorphisms (rs5882, rs1800775, rs3764261, rs12149545, rs711752, and rs708272) by using the Snapshot method. Results: (1) Significant differences were found involving the frequency distribution of genotypes and alleles of rs1800775, rs3764261, rs12149545, rs711752, and rs708272 between the control and MS groups (all p < 0.05). (2) rs1800775, rs3764261, rs12149545, rs711752, and rs708272 polymorphisms were significantly related to the risk of MS (all p < 0.05). (3) The rs1800775 polymorphism was associated with high fasting blood glucose levels and low high density lipoprotein cholesterol (HDL-C); rs3764261 and rs12149545 polymorphisms were associated with all components of MS except high blood pressure; rs711752 and rs708272 polymorphisms were associated with low HDL-C (all p < 0.05). (4) Complete linkage disequilibrium (LD) was identified for two pairs of single nucleotide polymorphisms (SNPs) (rs3764261 and rs12149545 (D’ = 1.000, r2 = 0.931), rs711752 and rs708272 (D’ = 1.000, r2 = 0.996)). (5) The A-G-G-G-C (p = 0.013, odds ratio [OR] = 0.622, 95% confidence interval [95% CI] = 0.427–0.906) and A-T-A-A-T (p < 0.001, OR = 0.519, 95% CI = 0.386–0.697) haplotypes were more frequent in the control group than in the case group. Conclusions: The rs1800775, rs3764261, rs12149545, rs711752, and rs708272 polymorphisms of CETP were associated with MS and its components among the Uyghur ethnic group. Complete LD was found between two pairs of SNPs (rs3764261 and rs12149545, rs711752, and rs708272). The A-G-G-G-C and A-T-A-A-T haplotypes might be protective factors for MS.
Attachment Style Is Related to Quality of Life for Assistance Dog Owners
Authors: White Naomi , Mills Daniel , Hall Sophie
Published: June 2017

Attachment styles have been shown to affect quality of life. Growing interest in the value of companion animals highlights that owning a dog can also affect quality of life, yet little research has explored the role of the attachment bond in affecting the relationship between dog ownership and quality of life. Given that the impact of dog ownership on quality of life may be greater for assistance dog owners than pet dog owners, we explored how anxious attachment and avoidance attachment styles to an assistance dog affected owner quality of life (n = 73). Regression analysis revealed that higher anxious attachment to the dog predicted enhanced quality of life. It is suggested that the unique, interdependent relationship between an individual and their assistance dog may mean that an anxious attachment style is not necessarily detrimental. Feelings that indicate attachment insecurity in other relationships may reflect more positive aspects of the assistance dog owner relationship, such as the level of support that the dog provides its owner.
Effects of Sulfate, Chloride, and Bicarbonate on Iron Stability in a PVC-U Drinking Pipe
Authors: Wang Jiaying , Tao Tao , Yan Hexiang
Published: June 2017

In order to describe iron stability in plastic pipes and to ensure the drinking water security, the influence factors and rules for iron adsorption and release were studied, dependent on the Unplasticized poly (vinyl chloride) (PVC-U) drinking pipes employed in this research. In this paper, sulfate, chloride, and bicarbonate, as well as synthesized models, were chosen to investigate the iron stability on the inner wall of PVC-U drinking pipes. The existence of the three kinds of anions could significantly affect the process of iron adsorption, and a positive association was found between the level of anion concentration and the adsorption rate. However, the scaling formed on the inner surface of the pipes would be released into the water under certain conditions. The Larson Index (LI), used for a synthetic consideration of anion effects on iron stability, was selected to investigate the iron release under multi-factor conditions. Moreover, a well fitted linear model was established to gain a better understanding of iron release under multi-factor conditions. The simulation results demonstrated that the linear model was better fitted than the LI model for the prediction of iron release.
The Instrumentation of a Microfluidic Analyzer Enabling the Characterization of the Specific Membrane Capacitance, Cytoplasm Conductivity, and Instantaneous Young’s Modulus of Single Cells
Authors: Wang Ke , Zhao Yang , Chen Deyong , Huang Chengjun , Fan Beiyuan , Long Rong , Hsieh Chia-Hsun , Wang Junbo , Wu Min-Hsien , Chen Jian
Published: June 2017

This paper presents the instrumentation of a microfluidic analyzer enabling the characterization of single-cell biophysical properties, which includes seven key components: a microfluidic module, a pressure module, an imaging module, an impedance module, two LabVIEW platforms for instrument operation and raw data processing, respectively, and a Python code for data translation. Under the control of the LabVIEW platform for instrument operation, the pressure module flushes single cells into the microfluidic module with raw biophysical parameters sampled by the imaging and impedance modules and processed by the LabVIEW platform for raw data processing, which were further translated into intrinsic cellular biophysical parameters using the code developed in Python. Based on this system, specific membrane capacitance, cytoplasm conductivity, and instantaneous Young’s modulus of three cell types were quantified as 2.76 ± 0.57 μF/cm2, 1.00 ± 0.14 S/m, and 3.79 ± 1.11 kPa for A549 cells (ncell = 202); 1.88 ± 0.31 μF/cm2, 1.05 ± 0.16 S/m, and 3.74 ± 0.75 kPa for 95D cells (ncell = 257); 2.11 ± 0.38 μF/cm2, 0.87 ± 0.11 S/m, and 5.39 ± 0.89 kPa for H460 cells (ncell = 246). As a semi-automatic instrument with a throughput of roughly 1 cell per second, this prototype instrument can be potentially used for the characterization of cellular biophysical properties.
Propolis Diterpenes as a Remarkable Bio-Source for Drug Discovery Development: A Review
Authors: Aminimoghadamfarouj Noushin , Nematollahi Alireza
Published: June 2017

Propolis is one of the complex, but valuable, bio-sources for discovering therapeutic compounds. Diterpenes are organic compounds composed of four isoprene units and are known for their biological and pharmacological characteristics, such as antibacterial, anticancer, and anti-inflammatory activities. Recently, advancements have been made in the development of antibacterial and anticancer leads from propolis-isolated diterpenes, and scrutiny of these compounds is being pursued. Thus, this review covers the progress in this arena, with a focus on the chemistry and biological activities of propolis diterpenes. It is anticipated that important information, in a comprehensive and concise manner, will be delivered here for better understanding of natural product drug discovery research.
EGFR and EGFRvIII Promote Angiogenesis and Cell Invasion in Glioblastoma: Combination Therapies for an Effective Treatment
Authors: Keller Stefanie , Schmidt Mirko
Published: June 2017

Epidermal growth factor receptor (EGFR) and the mutant EGFRvIII are major focal points in current concepts of targeted cancer therapy for glioblastoma multiforme (GBM), the most malignant primary brain tumor. The receptors participate in the key processes of tumor cell invasion and tumor-related angiogenesis and their upregulation correlates with the poor prognosis of glioma patients. Glioma cell invasion and increased angiogenesis share mechanisms of the degradation of the extracellular matrix (ECM) through upregulation of ECM-degrading proteases as well as the activation of aberrant signaling pathways. This review describes the role of EGFR and EGFRvIII in those mechanisms which might offer new combined therapeutic approaches targeting EGFR or EGFRvIII together with drug treatments against proteases of the ECM or downstream signaling to increase the inhibitory effects of mono-therapies.
Knockout of Murine Mamld1 Impairs Testicular Growth and Daily Sperm Production but Permits Normal Postnatal Androgen Production and Fertility
Authors: Miyado Mami , Yoshida Kaoru , Miyado Kenji , Katsumi Momori , Saito Kazuki , Nakamura Shigeru , Ogata Tsutomu , Fukami Maki
Published: June 2017

MAMLD1 has been implicated in testicular function in both human and mouse fetuses. Although three patients with MAMLD1 mutations were reported to have hypergonadotropic hypogonadism in their teens, the functional significance of MAMLD1 in the postnatal testis remains unclear. Here, we analyzed the phenotype of Mamld1 knockout (KO) male mice at reproductive ages. The reproductive organs of KO male mice were morphologically unremarkable, except for relatively small testes. Seminiferous tubule size and number of proliferating spermatogonia/spermatocytes were reduced in the KO testis. Daily sperm production of KO mice was mildly attenuated, whereas total sperm counts in epididymal semen remained normal. Sperm motility and morphology, as well as androgen levels in serum and testicular tissues and the number of pups born from cross-mated wildtype (WT) female mice, were comparable between WT and KO male mice. These results indicate that MAMLD1 contributes to the maintenance of postnatal testicular growth and daily sperm production but is dispensable for androgen biosynthesis and fertility. MAMLD1 likely plays supporting roles in multiple and continuous steps of male reproduction.
Suppression of Ghrelin Exacerbates HFCS-Induced Adiposity and Insulin Resistance
Authors: Ma Xiaojun , Lin Ligen , Yue Jing , Wu Chia-Shan , Guo Cathy , Wang Ruitao , Yu Kai-Jiang , Devaraj Sridevi , Murano Peter , Chen Zheng , Sun Yuxiang
Published: June 2017

High fructose corn syrup (HFCS) is widely used as sweetener in processed foods and soft drinks in the United States, largely substituting sucrose (SUC). The orexigenic hormone ghrelin promotes obesity and insulin resistance; ghrelin responds differently to HFCS and SUC ingestion. Here we investigated the roles of ghrelin in HFCS- and SUC-induced adiposity and insulin resistance. To mimic soft drinks, 10-week-old male wild-type (WT) and ghrelin knockout (Ghrelin−/−) mice were subjected to ad lib. regular chow diet supplemented with either water (RD), 8% HFCS (HFCS), or 10% sucrose (SUC). We found that SUC-feeding induced more robust increases in body weight and body fat than HFCS-feeding. Comparing to SUC-fed mice, HFCS-fed mice showed lower body weight but higher circulating glucose and insulin levels. Interestingly, we also found that ghrelin deletion exacerbates HFCS-induced adiposity and inflammation in adipose tissues, as well as whole-body insulin resistance. Our findings suggest that HFCS and SUC have differential effects on lipid metabolism: while sucrose promotes obesogenesis, HFCS primarily enhances inflammation and insulin resistance, and ghrelin confers protective effects for these metabolic dysfunctions.
The Role of HCG in Implantation: A Mini-Review of Molecular and Clinical Evidence
Authors: Makrigiannakis Antonis , Vrekoussis Thomas , Zoumakis Emmanouel , Kalantaridou Sophia , Jeschke Udo
Published: June 2017

Embryo implantation is a complex process involving continuous molecular cross-talk between the embryo and the decidua. One of the key molecules during this process is human chorionic gonadotropin (HCG). HCG effectively modulates several metabolic pathways within the decidua contributing to endometrial receptivity. Herein, a brief overview of the molecular mechanisms regulated by HCG is presented. Furthermore, we summarize the existing evidence regarding the clinical impact on reproductive outcomes after endometrial priming with HCG prior to embryo transfer. Although promising, further evidence is needed to clarify the protocol that would lead to beneficial outcomes.
Fortifying Horticultural Crops with Essential Amino Acids: A Review
Authors: Wang Guoping , Xu Mengyun , Wang Wenyi , Galili Gad
Published: June 2017

To feed the world′s growing population, increasing the yield of crops is not the only important factor, improving crop quality is also important, and it presents a significant challenge. Among the important crops, horticultural crops (particularly fruits and vegetables) provide numerous health compounds, such as vitamins, antioxidants, and amino acids. Essential amino acids are those that cannot be produced by the organism and, therefore, must be obtained from diet, particularly from meat, eggs, and milk, as well as a variety of plants. Extensive efforts have been devoted to increasing the levels of essential amino acids in plants. Yet, these efforts have been met with very little success due to the limited genetic resources for plant breeding and because high essential amino acid content is generally accompanied by limited plant growth. With a deep understanding of the biosynthetic pathways of essential amino acids and their interactions with the regulatory networks in plants, it should be possible to use genetic engineering to improve the essential amino acid content of horticultural plants, rendering these plants more nutritionally favorable crops. In the present report, we describe the recent advances in the enhancement of essential amino acids in horticultural plants and possible future directions towards their bio-fortification.
Candida albicans Impairments Induced by Peppermint and Clove Oils at Sub-Inhibitory Concentrations
Authors: Rajkowska Katarzyna , Otlewska Anna , Kunicka-Styczyńska Alina , Krajewska Agnieszka
Published: June 2017

Members of Candida species cause significant health problems, inducing various types of superficial and deep-seated mycoses in humans. In order to prevent from Candida sp. development, essential oils are more and more frequently applied, due to their antifungal activity, low toxicity if used appropriately, and biodegrability. The aim of the study was to characterize the early alterations in Candida albicans metabolic properties in relation to proteins and chromosomal DNA profiles, after treatment with peppermint and clove oils at sub-inhibitory concentrations. The yeasts were affected by the oils even at a concentration of 0.0075% v/v, which resulted in changes in colony morphotypes and metabolic activities. Peppermint and clove oils at concentrations ranging from 0.015× MIC (minimal inhibitory concentration) to 0.5× MIC values substantially affected the enzymatic abilities of C. albicans, and these changes were primarily associated with the loss or decrease of activity of all 9 enzymes detected in the untreated yeast. Moreover, 29% isolates showed additional activity of N-acetyl-β-glucosaminidase and 14% isolates—α-fucosidase in comparison to the yeast grown without essential oils addition. In response to essential oils at 0.25–0.5× MIC, extensive changes in C. albicans whole-cell protein profiles were noted. However, the yeast biochemical profiles were intact with the sole exception of the isolate treated with clove oil at 0.5× MIC. The alterations were not attributed to gross chromosomal rearrangements in C. albicans karyotype. The predominantly observed decrease in protein fractions and the yeast enzymatic activity after treatment with the oils should be considered as a phenotypic response of C. albicans to the essential oils at their sub-inhibitory concentrations and may lead to the reduction of this yeast pathogenicity.
Reply to the Letter to the Editor by C. Nicolazzo et al.: “Circulating Cell-Free DNA and Circulating Tumor Cells as Prognostic and Predictive Biomarkers in Advanced Non-Small Cell Lung Cancer Patients Treated with First-Line Chemotherapy”
Authors: Alama Angela , Coco Simona , Vanni Irene , Grossi Francesco
Published: June 2017

Reply: Thank you for the valuable comments. We agree with the concern that distinguishing genuine circulating tumor cells (CTCs) from other circulating cells by morphology may be questionable.[...]
Ultra High Strain Rate Nanoindentation Testing
Authors: Sudharshan Phani Pardhasaradhi , Oliver Warren
Published: June 2017

Strain rate dependence of indentation hardness has been widely used to study time-dependent plasticity. However, the currently available techniques limit the range of strain rates that can be achieved during indentation testing. Recent advances in electronics have enabled nanomechanical measurements with very low noise levels (sub nanometer) at fast time constants (20 µs) and high data acquisition rates (100 KHz). These capabilities open the doors for a wide range of ultra-fast nanomechanical testing, for instance, indentation testing at very high strain rates. With an accurate dynamic model and an instrument with fast time constants, step load tests can be performed which enable access to indentation strain rates approaching ballistic levels (i.e., 4000 1/s). A novel indentation based testing technique involving a combination of step load and constant load and hold tests that enables measurement of strain rate dependence of hardness spanning over seven orders of magnitude in strain rate is presented. A simple analysis is used to calculate the equivalent uniaxial response from indentation data and compared to the conventional uniaxial data for commercial purity aluminum. Excellent agreement is found between the indentation and uniaxial data over several orders of magnitude of strain rate.
Biosynthesis and Characterization of AgNPs–Silk/PVA Film for Potential Packaging Application
Authors: Tao Gang , Cai Rui , Wang Yejing , Song Kai , Guo Pengchao , Zhao Ping , Zuo Hua , He Huawei
Published: June 2017

Bionanocomposite packaging materials have a bright future for a broad range of applications in the food and biomedical industries. Antimicrobial packaging is one of the bionanocomposite packaging materials. Silver nanoparticle (AgNP) is one of the most attractive antimicrobial agents for its broad spectrum of antimicrobial activity against microorganisms. However, the traditional method of preparing AgNPs-functionalized packaging material is cumbersome and not environmentally friendly. To develop an efficient and convenient biosynthesis method to prepare AgNPs-modified bionanocomposite material for packaging applications, we synthesized AgNPs in situ in a silk fibroin solution via the reduction of Ag+ by the tyrosine residue of fibroin, and then prepared AgNPs–silk/poly(vinyl alcohol) (PVA) composite film by blending with PVA. AgNPs were synthesized evenly on the surface or embedded in the interior of silk/PVA film. The prepared AgNPs–silk/PVA film exhibited excellent mechanical performance and stability, as well as good antibacterial activity against both Gram-negative and Gram-positive bacteria. AgNPs–silk/PVA film offers more choices to be potentially applied in the active packaging field.
Study on Temper Embrittlement and Hydrogen Embrittlement of a Hydrogenation Reactor by Small Punch Test
Authors: Guan Kaishu , Szpunar Jerzy A. , Matocha Karel , Wang Duwei
Published: June 2017

The study on temper embrittlement and hydrogen embrittlement of a test block from a 3Cr1Mo1/4V hydrogenation reactor after ten years of service was carried out by small punch test (SPT) at different temperatures. The SPT fracture energy Esp (derived from integrating the load-displacement curve) divided by the maximum load (Fm) of SPT was used to fit the Esp/Fm versus-temperature curve to determine the energy transition temperature (Tsp) which corresponded to the ductile-brittle transition temperature of the Charpy impact test. The results indicated that the ratio of Esp/Fm could better represent the energy of transition in SPT compared with Esp. The ductile-to-brittle transition temperature of the four different types of materials was measured using the hydrogen charging test by SPT. These four types of materials included the base metal and the weld metal in the as-received state, and the base metal and the weld metal in the de-embrittled state. The results showed that there was a degree of temper embrittlement in the base metal and the weld metal after ten years of service at 390 °C. The specimens became slightly more brittle but this was not obvious after hydrogen charging. Because the toughness of the material of the hydrogenation reactor was very good, the flat samples of SPT could not characterize the energy transition temperature within the liquid nitrogen temperature. Additionally, there was no synergetic effect of temper embrittlement and hydrogen embrittlement found in 3Cr1Mo1/4V steel.