bluuebunny/crossref_metadata_2025 huggingface dataset:
Quantification of the Li-ion diffusion over an interface coating in all-solid-state batteries via NMR measurements
Authors: Liu Ming , Wang Chao , Zhao Chenglong , van der Maas Eveline , Lin Kui , Arszelewska Violetta A. , Li Baohua , Ganapathy Swapna , Wagemaker Marnix
Published: October 2021

A key challenge for solid-state-batteries development is to design electrode-electrolyte interfaces that combine (electro)chemical and mechanical stability with facile Li-ion transport. However, while the solid-electrolyte/electrode interfacial area should be maximized to facilitate the transport of high electrical currents on the one hand, on the other hand, this area should be minimized to reduce the parasitic interfacial reactions and promote the overall cell stability. To improve these aspects simultaneously, we report the use of an interfacial inorganic coating and the study of its impact on the local Li-ion transport over the grain boundaries. Via exchange-NMR measurements, we quantify the equilibrium between the various phases present at the interface between an S-based positive electrode and an inorganic solid-electrolyte. We also demonstrate the beneficial effect of the LiI coating on the all-solid-state cell performances, which leads to efficient sulfur activation and prevention of solid-electrolyte decomposition. Finally, we report 200 cycles with a stable capacity of around 600 mAh g−1 at 0.264 mA cm−2 for a full lab-scale cell comprising of LiI-coated Li2S-based cathode, Li-In alloy anode and Li6PS5Cl solid electrolyte.
Enhanced North Pacific subtropical gyre circulation during the late Holocene
Authors: Zhang Yancheng , Zheng Xufeng , Kong Deming , Yan Hong , Liu Zhonghui
Published: October 2021

The North Pacific Subtropical Gyre circulation redistributes heat from the Western Pacific Warm Pool towards the mid- to high-latitude North Pacific. However, the driving mechanisms of this circulation and how it changed over the Holocene remain poorly understood. Here, we present alkenone-based sea surface temperature reconstructions along the Kuroshio, California and Alaska currents that cover the past ~7,000 years. These and other paleorecords collectively demonstrate a coherent intensification of the boundary currents, and thereby the basin-scale subtropical gyre circulation, since ~3,000–4,000 years ago. Such enhanced circulation during the late Holocene appears to have resulted from a long-term southward migration of the Intertropical Convergence Zone, associated with Holocene ocean cooling. Our results imply that the North Pacific Subtropical Gyre circulation could be weakened under future global warming.
Indian Ocean Dipole leads to Atlantic Niño
Authors: Zhang Lei , Han Weiqing
Published: October 2021

Atlantic Niño is the Atlantic equivalent of El Niño-Southern Oscillation (ENSO), and it has prominent impacts on regional and global climate. Existing studies suggest that the Atlantic Niño may arise from local atmosphere-ocean interaction and is sometimes triggered by the Atlantic Meridional Mode (AMM), with overall weak ENSO contribution. By analyzing observational datasets and performing numerical model experiments, here we show that the Atlantic Niño can be induced by the Indian Ocean Dipole (IOD). We find that the enhanced rainfall in the western tropical Indian Ocean during positive IOD weakens the easterly trade winds over the tropical Atlantic, causing warm anomalies in the central and eastern equatorial Atlantic basin and therefore triggering the Atlantic Niño. Our finding suggests that the cross-basin impact from the tropical Indian Ocean plays a more important role in affecting interannual climate variability than previously thought.
Coupling complementary strategy to flexible graph neural network for quick discovery of coformer in diverse co-crystal materials
Authors: Jiang Yuanyuan , Yang Zongwei , Guo Jiali , Li Hongzhen , Liu Yijing , Guo Yanzhi , Li Menglong , Pu Xuemei
Published: October 2021

Cocrystal engineering have been widely applied in pharmaceutical, chemistry and material fields. However, how to effectively choose coformer has been a challenging task on experiments. Here we develop a graph neural network (GNN) based deep learning framework to quickly predict formation of the cocrystal. In order to capture main driving force to crystallization from 6819 positive and 1052 negative samples reported by experiments, a feasible GNN framework is explored to integrate important prior knowledge into end-to-end learning on the molecular graph. The model is strongly validated against seven competitive models and three challenging independent test sets involving pharmaceutical cocrystals, π–π cocrystals and energetic cocrystals, exhibiting superior performance with accuracy higher than 96%, confirming its robustness and generalization. Furthermore, one new energetic cocrystal predicted is successfully synthesized, showcasing high potential of the model in practice. All the data and source codes are available at https://github.com/Saoge123/ccgnet for aiding cocrystal community.
Wafer-scale functional circuits based on two dimensional semiconductors with fabrication optimized by machine learning
Authors: Chen Xinyu , Xie Yufeng , Sheng Yaochen , Tang Hongwei , Wang Zeming , Wang Yu , Wang Yin , Liao Fuyou , Ma Jingyi , Guo Xiaojiao , Tong Ling , Liu Hanqi , Liu Hao , Wu Tianxiang , Cao Jiaxin , Bu Sitong , Shen Hui , Bai Fuyu , Huang Daming , Deng Jianan , Riaud Antoine , Xu Zihan , Wu Chenjian , Xing Shiwei , Lu Ye , Ma Shunli , Sun Zhengzong , Xue Zhongyin , Di Zengfeng , Gong Xiao , Zhang David Wei , Zhou Peng , Wan Jing , Bao Wenzhong
Published: October 2021

Triggered by the pioneering research on graphene, the family of two-dimensional layered materials (2DLMs) has been investigated for more than a decade, and appealing functionalities have been demonstrated. However, there are still challenges inhibiting high-quality growth and circuit-level integration, and results from previous studies are still far from complying with industrial standards. Here, we overcome these challenges by utilizing machine-learning (ML) algorithms to evaluate key process parameters that impact the electrical characteristics of MoS2 top-gated field-effect transistors (FETs). The wafer-scale fabrication processes are then guided by ML combined with grid searching to co-optimize device performance, including mobility, threshold voltage and subthreshold swing. A 62-level SPICE modeling was implemented for MoS2 FETs and further used to construct functional digital, analog, and photodetection circuits. Finally, we present wafer-scale test FET arrays and a 4-bit full adder employing industry-standard design flows and processes. Taken together, these results experimentally validate the application potential of ML-assisted fabrication optimization for beyond-silicon electronic materials.
Estimation of cell-free fetal DNA fraction from maternal plasma based on linkage disequilibrium information
Authors: Ju Jia , Li Jia , Liu Siyang , Zhang Haiqiang , Xu Jinjin , Lin Yu , Gao Ya , Zhou Yulin , Jin Xin
Published: October 2021

Cell-free fetal DNA fraction (FF) in maternal plasma is a key parameter affecting the performance of noninvasive prenatal testing (NIPT). Accurate quantitation of FF plays a pivotal role in these tests. However, there are few methods that could determine FF with high accuracy using shallow‐depth whole‐genome sequencing data. In this study, we hypothesized that the actual FF in maternal plasma should be proportional to the discrepancy rate between the observed genotypes and inferred genotypes based on the linkage disequilibrium rule in certain polymorphism sites. Based on this hypothesis, we developed a method named Linkage Disequilibrium information-based cell-free Fetal DNA Fraction (LDFF) to accurately quantify FF in maternal plasma. This method achieves a high performance and outperforms existing methods in the fetal DNA fraction estimation. As LDFF is a gender-independent method and developed on shallow-depth samples, it can be easily incorporated into routine NIPT test and may enhance the current NIPT performance.
Daple deficiency causes hearing loss in adult mice by inducing defects in cochlear stereocilia and apical microtubules
Authors: Ozono Yoshiyuki , Tamura Atsushi , Nakayama Shogo , Herawati Elisa , Hanada Yukiko , Ohata Kazuya , Takagishi Maki , Takahashi Masahide , Imai Takao , Ohta Yumi , Oshima Kazuo , Sato Takashi , Inohara Hidenori , Tsukita Sachiko
Published: October 2021

The V-shaped arrangement of hair bundles on cochlear hair cells is critical for auditory sensing. However, regulation of hair bundle arrangements has not been fully understood. Recently, defects in hair bundle arrangement were reported in postnatal Dishevelled-associating protein (ccdc88c, alias Daple)-deficient mice. In the present study, we found that adult Daple−/− mice exhibited hearing disturbances over a broad frequency range through auditory brainstem response testing. Consistently, distorted patterns of hair bundles were detected in almost all regions, more typically in the basal region of the cochlear duct. In adult Daple−/− mice, apical microtubules were irregularly aggregated, and the number of microtubules attached to plasma membranes was decreased. Similar phenotypes were manifested upon nocodazole treatment in a wild type cochlea culture without affecting the microtubule structure of the kinocilium. These results indicate critical role of Daple in hair bundle arrangement through the orchestration of apical microtubule distribution, and thereby in hearing, especially at high frequencies.
Clinical spectrum and the comorbidities of Dravet syndrome in Taiwan and the possible molecular mechanisms
Authors: Huang Chia-Hsuan , Hung Pi-Lien , Fan Pi-Chuan , Lin Kuang-Lin , Hsu Ting-Rong , Chou I-Jun , Ho Che-Sheng , Chou I-Ching , Lin Wei-Sheng , Lee Inn-Chi , Fan Hueng-Chuen , Chen Shyi-Jou , Liang Jao-Shwann , Tu Yi-Fang , Chang Tung-Ming , Hu Su-Ching , Wong Lee-Chin , Hung Kun-Long , Lee Wang-Tso
Published: October 2021

Dravet syndrome (DS) is an uncommon epilepsy syndrome that may negatively affect the patients and their caregivers. However, reliable and valid measures of its impact on caregivers and the characteristics of patients with DS in Taiwan are lacking. This study aimed to describe the characteristics of patients with DS and concerns of their caregivers and establish a baseline frequency of disease characteristics using a cross-sectional survey in Taiwan. We assessed the caregivers of patients with DS using an online anonymous questionnaire. The seizure frequency decreased with age, although lacking statistical significance. Vaccines show no influence on the condition of patients with DS. Our findings revealed the highest impact on the domains affecting the caregivers’ daily life, including additional household tasks, symptom observation, further medical plan, and financial issues. Caregivers also expressed concerns regarding the lack of independence/constant care, seizure control, speech/communication, and impacts on siblings because of long-term care of the patients in parents’ absence. Our findings highlight the significant effects of caring for a child with DS on the lives of their caregivers in Taiwan; these findings will help raise awareness regarding the needs of these families. Furthermore, we discussed the possible pathophysiological mechanisms of associated comorbidities.
Annual changes in the Biodiversity Intactness Index in tropical and subtropical forest biomes, 2001–2012
Authors: De Palma Adriana , Hoskins Andrew , Gonzalez Ricardo E. , Börger Luca , Newbold Tim , Sanchez-Ortiz Katia , Ferrier Simon , Purvis Andy
Published: October 2021

Few biodiversity indicators are available that reflect the state of broad-sense biodiversity—rather than of particular taxa—at fine spatial and temporal resolution. One such indicator, the Biodiversity Intactness Index (BII), estimates how the average abundance of the native terrestrial species in a region compares with their abundances in the absence of pronounced human impacts. We produced annual maps of modelled BII at 30-arc-second resolution (roughly 1 km at the equator) across tropical and subtropical forested biomes, by combining annual data on land use, human population density and road networks, and statistical models of how these variables affect overall abundance and compositional similarity of plants, fungi, invertebrates and vertebrates. Across tropical and subtropical biomes, BII fell by an average of 1.9 percentage points between 2001 and 2012, with 81 countries seeing an average reduction and 43 an average increase; the extent of primary forest fell by 3.9% over the same period. We did not find strong relationships between changes in BII and countries’ rates of economic growth over the same period; however, limitations in mapping BII in plantation forests may hinder our ability to identify these relationships. This is the first time temporal change in BII has been estimated across such a large region.
Huygens' Principle geometric derivation and elimination of the wake and backward wave
Authors: Anderson Forrest L.
Published: October 2021

Huygens' Principle (1678) implies that every point on a wave front serves as a source of secondary wavelets, and the new wave front is the tangential surface to all the secondary wavelets. But two problems arise: portions of wavelets that exist outside of the new wave front combine to form a wake. Also there are two tangential surfaces so wave fronts are propagated in both the forward and backward directions. These problems have not previously been resolved by using a geometrical theory with impulsive wavelets that are in harmony with Huygens' geometrical description. Doing so would provide deeper understanding of and greater intuition into wave propagation, in addition to providing a new model for wave propagation analysis. The interpretation, developed here, of Huygens' geometrical construction shows Huygens' Principle to be correct: as for the wake, the Huygens' wavelets disappear when combined except where they contact their common tangent surfaces, the new propagating wave fronts. As for the backward wave, a source propagates both a forward wave and a backward wave when it is stationary, but it propagates only the forward wave front when it is advancing with a speed equal to the propagation speed of the wave fronts.
Effect of small heat release and viscosity on thermal-diffusive instability
Authors: Wada Keigo
Published: October 2021

The linear stability of a thermal reaction front has been investigated based on the thermal-diffusive model proposed by Zel’dovich and Frank-Kamenetskii, which is called ZFK model. In the framework of ZFK model, heat-conduction and mass-diffusion equations are treated without the effect of hydrodynamic flow. Then, two types of instability appear: cellular and oscillatory instabilities. The cellular instability has only positive real part of growth rate, while the oscillatory instability is accompanied with non-zero imaginary part. In this study, the effect of heat release and viscosity on both instabilities is investigated asymptotically and numerically. This is achieved by coupling mass-conservation and Navier–Stokes equations with the ZFK model for small heat release. Then, the stable range of Lewis number, where the real part of growth rate is negative, is widened by non-zero values of heat release for any wavenumber. The increase of Prandtl number also brings the stabilization effect on the oscillatory instability. However, as for the cellular instability, the viscosity leads to the destabilization effect for small wavenumbers, opposed to its stabilization effect for moderate values of wavenumber. Under the limit of small wavenumber, the property of viscosity becomes clear in view of cut-off wavenumber, which makes the real part of growth rate zero.
Swallowing, nutritional status, and salivary flow in patients after head and neck cancer treatment, a pilot study
Authors: de Carvalho Mariana Inri , Gatti Marina , Guedes Renata Ligia Vieira , Froes Renata Camilla Favarin , Costa Danila Rodrigues , da Silva Vitor Jhonatan , da Silva Santos Paulo Sérgio , Berretin-Felix Giédre
Published: October 2021

Determine the relationship between swallowing function, nutritional status, and salivary flow in patients after head and neck cancer treatment. This pilot study included 17 patients. Swallowing was assessed through videofluoroscopy and surface electromyography (sEMG), nutritional status through anthropometry and dietary assessment, and salivary flow both with and without mechanical stimulation. Test analysis showed that 66.7% of patients had functional limitations in swallowing in 58.3%, 66.7%, and 58.3% residue scale with an average of a line of barium on a structure for pudding, honey, and liquid consistencies, respectively. Laryngeal penetration was found in 8.3% during the swallowing of liquid. Surface electromyography (sEMG) showed above normal values for muscle activity time during the swallowing of pudding. Anthropometric assessment and muscle and adipose tissue indicated eutrophy. Salivary flow test with mechanical stimulus showed that 82.3% of patients' salivary production was well below the appropriate level. There was a significant correlation between muscle tissue reserve and muscle activity time during swallowing in the studied muscles (left masseter p = 0.003, right masseter p = 0.001, suprahyoid p = 0.001, orbicularis oris = 0.020), all in pudding consistency. This pilot study confirmed the relationship between swallowing and nutritional status for its participants, showing that appropriate protein intake influences muscle activity during swallowing in head and neck cancer survivors.
Higher-order topological Mott insulator on the pyrochlore lattice
Authors: Otsuka Yuichi , Yoshida Tsuneya , Kudo Koji , Yunoki Seiji , Hatsugai Yasuhiro
Published: October 2021

We provide the first unbiased evidence for a higher-order topological Mott insulator in three dimensions by numerically exact quantum Monte Carlo simulations. This insulating phase is adiabatically connected to a third-order topological insulator in the noninteracting limit, which features gapless modes around the corners of the pyrochlore lattice and is characterized by a $${\mathbb {Z}}_{4}$$

Z
4

spin-Berry phase. The difference between the correlated and non-correlated topological phases is that in the former phase the gapless corner modes emerge only in spin excitations being Mott-like. We also show that the topological phase transition from the third-order topological Mott insulator to the usual Mott insulator occurs when the bulk spin gap solely closes.
Comparison of bioactive compounds and health promoting properties of fruits and leaves of apple, pear and quince
Authors: Wojdyło Aneta , Nowicka Paulina , Turkiewicz Igor Piotr , Tkacz Karolina , Hernandez Francisca
Published: October 2021

This paper presents characterization of healthy potential new sources of functional constituents with reference to basic plant sources. In this study, the phenolics, triterpene, isoprenoids (chlorophylls and carotenoids), amino acids, minerals, sugars and organic acids of different cultivars of pome species—apple, pear, quince—leaves vs. fruits and their enzymatic in vitro enzyme inhibition of hyperglycemic (α-glucosidase, α-amylase), obesity (pancreatic lipase), cholinesterase (acetylcholinesterase, butylcholinesterase), inflammatory (15-LOX, COX-1 and -2) and antioxidant capacity (ORAC, FRAP, ABTS) were evaluated. Leaves of pome species as a new plant sources were characterized by higher content of bioactive and nutritional compounds than basic fruits. The dominant fraction for quince, pear, and apple fruits was polymeric procyanidins. In quince and pear leaves flavan-3-ols, and in apple dihydrochalcones dominated. Triterpene was present in equal content in leaves and fruits. Leaves are excellent sources of amino acids and minerals (especially Ca, Mg, Fe, and K), with high content of organic acids and low content of sugars compared to fruits of pome species. Leaves of apples and pears most effectively inhibited COX-1, COX-2, α-amylase, and α-glucosidase enzyme but quince leaves showed the most effective inhibition of pancreatic lipase, AChE and BuChE, 15-LOX, and antioxidant capacity, which particularly correlated with bioactive compounds. Present study shows that leaves are promising sources of valuable compounds and may be used to produce functional foods as well as for medical purposes.
Optimization of a novel lipid extraction process from microalgae
Authors: Ren Xiaojie , Wei Chao , Yan Qi , Shan Xin , Wu Mengyun , Zhao Xinhe , Song Yuanda
Published: October 2021

Previous study found that the solvent extraction efficiency of lipid in microalgae could be greatly improved by washing algae cells before the second time extraction. Based on the "organic solvents–water–organic solvents" method, this research further studied the effect of four solvent systems (acetone, chloroform/methanol, chloroform/methanol/water, dichloromethane/methanol), two types of water treatment (vortex and ultrasonic), three water treatment time gradient (0 s, 30 s, 120 s) on the lipid extraction at three different microalgae growth stages (3rd day, 5th day, 9th day). The results show that the combination of water treatment type, treatment time and solvent is very important to the efficiency of lipid extraction. The total lipid extracted was generally increased by 10–30% after water treatment. Especially under the condition of 120 s vortex water treatment with dichloromethane/methanol as extraction solvent, the total lipid extracted increased by 61.14%. In addition, microalgae cells at different culture stages had different sensitivity to water treatment. In this study, under the combination of chloroform/methanol/water as extraction solvent and vortex water treatment for 120 s, the highest lipid yield was obtained on the ninth day of cell culture, which accounts 47.88% of the cell dry weight (478 mg/g cell dry weight). The changes of cell morphology and structure after water treatment were studied by scanning electron microscope, and it was found that water treatment could seriously destroy the cell membrane damaged by solvent, thus promoting the release of lipids. This study further optimizes the "solvent–water–solvent" lipid extraction method, which neither produces impurities nor damages the lipid quality, and can reduce the amount of organic solvent applied in the classical lipid extraction method with the same lipid yield, so it has a broad application prospect.
Effects of coalescence and isospin symmetry on the freezeout of light nuclei and their anti-particles
Authors: Waqas M. , Peng G. X. , Liu Fu-Hu , Wazir Z.
Published: October 2021

The transverse momentum spectra of light nuclei (deuteron, triton and helion) produced in various centrality intervals in Gold–Gold (Au–Au), Lead–Lead (Pb–Pb) and proton–Lead (p–Pb) collisions, as well as in inelastic (INEL) proton–proton (p–p) collisions are analyzed by the blast wave model with Boltzmann Gibbs statistics. The model results are nearly in agreement with the experimental data measured by STAR and ALICE Collaborations in special transverse momentum ranges. We extracted the bulk properties in terms of kinetic freezeout temperature, transverse flow velocity and freezeout volume. It is observed that deuteron and anti-deuteron freezeout later than triton and helion as well as their anti-particles due to its smaller mass, while helion and triton, and anti-helion and anti-triton freezeout at the same time due to isospin symmetry at higher energies. It is also observed that light nuclei freezeout earlier than their anti-nuclei due to the large coalescence of nucleons for light nuclei compared to their anti-nuclei. The kinetic freezeout temperature, transverse flow velocity and kinetic freezeout volume decrease from central to peripheral collisions. Furthermore, the transverse flow velocity depends on mass of the particle which decreases with increasing the mass of the particle.
Observational study on the efficiency of Neonatal Emergency Transport in reducing mortality and morbidity indexes in Sicily
Authors: Falsaperla Raffaele , Vitaliti Giovanna , Amato Barbara , Saporito Marco Andrea Nicola , Mauceri Laura , Sullo Federica , Motta Milena , Scalia Bruna , Puglisi Federica , Caccamo Martina , Longo Maria Grazia , Giacchi Valentina , Cimino Carla , Ruggieri Martino
Published: October 2021

In these last 25 years, the Neonatal Emergency Transport (NET) service has been widely improved in Italy. To date, all National areas are covered by a NET service; 53 NET centers have been activated in all the Italian territory. Herein, the authors present an observational study to evaluate the rate of infantile mortality after introduction of NET in Sicily, and to study the efficiency of this service in reducing these rates of mortality in vulnerable neonates, transported from primary care birth centers to tertiary facilities to undergo to specialized NICU assistance. All neonates who required an emergency transport by NETS were included. No exclusions criteria were applied. Demographic and regional infantile mortality data, expressed as infant mortality rate, were selected by the official government database (ISTAT- National Statistic Institute—http://www.istat.it). All data were respectively divided into three groups: data concerning transport, clinical condition, and mortality of the transported patients. We transported by NET 325 neonates. The analysis of the infant mortality rate (per 1.000 live births) in Catania from 2016 to 2018 was reduced compared to the same rate calculated before NETS activation (4.41 index before 2016 vs 4.17 index after 2016). These data showed an increase in other provinces (Enna, Caltanissetta, and Agrigento). 61% of neonates showed a respiratory disease. During the study period the proportion of neonates with a Mortality Index for Neonatal Transportation—MINT < 6 has been reduced, while there was an increase of neonates with higher Transport Risk Index of Physiologic Stability-TRIPS score results. The slight decrease of infantile mortality in Catania during the first three years after introduction of NET follows the same trend of all Italian territories, showing the importance of this service in reducing infantile mortality.
Unraveling the impacts of meteorological and anthropogenic changes on sediment fluxes along an estuary-sea continuum
Authors: Grasso Florent , Bismuth Eliott , Verney Romaric
Published: October 2021

Sediment fluxes at the estuary-sea interface strongly impact particle matter exchanges between marine and continental sources along the land-sea continuum. However, human activities drive pressures on estuary physical functioning, hence threatening estuarine habitats and their ecosystem services. This study explores a 22-year numerical hindcast of the macrotidal Seine Estuary (France), experiencing contrasted meteorological conditions and anthropogenic changes (i.e., estuary deepening and narrowing). The hindcast was thoroughly validated for both water column and sediment bed dynamics and showed good capacities to simulate annual sediment budgets observed from 1990 to 2015. We aim at disentangling the relative contributions of meteorological and human-induced morphological changes on net sediment fluxes between the estuary and its adjacent coastal sea. Our results highlight that intense wave events induce fine sediment (≤ 100 µm) export to the sea but coarser sediment (≥ 210 µm) import within the estuary. Although intense river discharges induce mud export to the sea, moderate to large river discharges prove to support mud import within the estuary. Wave and river discharge events were less intense in 2005–2015 than in 1990–2000, reducing fine sediment export to the sea. The estuary deepening and narrowing due to human activities increased fine sediment import within the estuary, shifting the estuary from an exporting to importing system. We propose a conceptualization of mud flux response to river discharge and wave forcing, as well as anthropogenic pressures. It provides valuable insights into particle transfers along the land-sea continuum, contributing to a better understanding of estuarine ecosystem trajectories under global changes.
Correlative studies on vitamin D and total, free bioavailable testosterone levels in young, healthy men
Authors: Książek Anna , Mędraś Marek , Zagrodna Aleksandra , Słowińska-Lisowska Małgorzata , Lwow Felicja
Published: October 2021

The relationship between vitamin D levels and testicular hormonal function in men has not been clearly established. Therefore, we aimed to investigate the relationship between deficiency/insufficiency levels of 25(OH)D and luteinizing hormone (LH), follicle-stimulating hormone (FSH), total (TT), free (FT), and bioavailable testosterone (BT), and sex hormone binding globulin (SHBG) in young, healthy men. We enrolled 176 healthy, active young men aged 18–35 years from a genetically homogenous population of Lower Silesia, Poland. Serum levels of 25(OH)D, LH, FSH, and TT were measured by electrochemiluminescence (ECLIA). FT levels were measured by enzyme-linked immunosorbent assay (ELISA). BT levels were calculated from TT, SHBG, and albumin. SHBG was measured by chemiluminescent immunoassay CLIA. We did not find any significant differences between the mean hormonal values (LH, FSH, TT, FT, BT, and SHBG) and the status of 25(OH)D level (deficient and insufficient). Based on our results, we concluded that there is no relationship between deficient and insufficient 25(OH)D concentration and androgen levels in young, healthy men.
One year of modeling and forecasting COVID-19 transmission to support policymakers in Connecticut
Authors: Morozova Olga , Li Zehang Richard , Crawford Forrest W.
Published: October 2021

To support public health policymakers in Connecticut, we developed a flexible county-structured compartmental SEIR-type model of SARS-CoV-2 transmission and COVID-19 disease progression. Our goals were to provide projections of infections, hospitalizations, and deaths, and estimates of important features of disease transmission and clinical progression. In this paper, we outline the model design, implementation and calibration, and describe how projections and estimates were used to meet the changing requirements of policymakers and officials in Connecticut from March 2020 to February 2021. The approach takes advantage of our unique access to Connecticut public health surveillance and hospital data and our direct connection to state officials and policymakers. We calibrated this model to data on deaths and hospitalizations and developed a novel measure of close interpersonal contact frequency to capture changes in transmission risk over time and used multiple local data sources to infer dynamics of time-varying model inputs. Estimated epidemiologic features of the COVID-19 epidemic in Connecticut include the effective reproduction number, cumulative incidence of infection, infection hospitalization and fatality ratios, and the case detection ratio. We conclude with a discussion of the limitations inherent in predicting uncertain epidemic trajectories and lessons learned from one year of providing COVID-19 projections in Connecticut.
Antibodies against phosphorylcholine in hospitalized versus non-hospitalized obese subjects
Authors: Jujić Amra , Korduner J. , Holm H. , Engström G. , Bachus E. , Bhattacharya P. , Nilsson P. M. , Frostegård Johan , Magnusson M.
Published: October 2021

Obesity associates with reduced life expectancy, type 2 diabetes, hypertension and cardiovascular disease, and is characterized by chronic inflammation. Phosphorylcholine (PC) is an epitope on oxidized low-density lipoprotein, dead cells and some microorganisms. Antibodies against PC (anti-PC) have anti-inflammatory properties. Here, we explored the role of anti-PC in hospitalized versus non-hospitalized obese. One-hundred-and-twenty-eight obese (BMI ≥ 30 kg/m2) individuals (59.8 (± 5.5) years, 53.9% women) from the Malmö Diet and Cancer Cardiovascular Cohort were examined and IgM, IgG1 and IgG2 anti-PC were analyzed by ELISA. Individuals with at least one recorded history of hospitalization prior to study baseline were considered hospitalized obese (HO). Associations between IgM, IgG1 and IgG2 anti-PC and HO (n = 32)/non-hospitalized obese (NHO) (n = 96), but also with metabolic syndrome and diabetes were analysed using logistic regressions. Both IgM and IgG1 anti-PC were inversely associated with HO, also after controlling for age and sex. When further adjusted for waist circumference, systolic blood pressure, glucose levels and smoking status, only IgG1 anti-PC remained significantly associated with HO. In multivariate models, each 1 standard deviation of increment in anti-PC IgG1 levels was inversely associated with prevalence of HO (odds ratio 0.57; CI 95% 0.33–0.98; p = 0.044). IgG2 anti-PC did not show any associations with HO. Low levels of IgM and IgG1 anti-PC are associated with higher risk of being a HO individual independent of sex and age, IgG1 anti-PC also independently of diabetes and metabolic syndrome. The anti-inflammatory properties of these antibodies may be related to inflammation in obesity and its complications.
Evaporation kinetics in highly porous tetrapodal zinc oxide networks studied using in situ SRµCT
Authors: Hindenlang Birte , Gapeeva Anna , Baum Martina J. , Kaps Sören , Saure Lena M. , Rasch Florian , Hammel Jörg , Moosmann Julian , Storm Malte , Adelung Rainer , Schütt Fabian , Zeller-Plumhoff Berit
Published: October 2021

Tetrapodal zinc oxide (t-ZnO) is used to fabricate polymer composites for many different applications ranging from biomedicine to electronics. In recent times, macroscopic framework structures from t-ZnO have been used as a versatile sacrificial template for the synthesis of multi-scaled foam structures from different nanomaterials such as graphene, hexagonal boron nitride or gallium nitride. Many of these fabrication methods rely on wet-chemical coating processes using nanomaterial dispersions, leading to a strong interest in the actual coating mechanism and factors influencing it. Depending on the type of medium (e.g. solvent) used, different results regarding the homogeneity of the nanomaterial coating can be achieved. In order to understand how a medium influences the coating behavior, the evaporation process of water and ethanol is investigated in this work using in situ synchrotron radiation-based micro computed tomography (SRµCT). By employing propagation-based phase contrast imaging, both the t-ZnO network and the medium can be visualized. Thus, the evaporation process can be monitored non-destructively in three dimensions. This investigation showed that using a polar medium such as water leads to uniform evaporation and, by that, a homogeneous coating of the entire network.
A study of depression, partnership and sexual satisfaction in patients with post-traumatic olfactory disorders
Authors: Kamrava Seyed Kamran , Tavakol Zeinab , Talebi Atefeh , Farhadi Mohammad , Jalessi Maryam , Hosseini Seyedeh Fahimeh , Amini Elahe , Chen Ben , Hummel Thomas , Alizadeh Rafieh
Published: October 2021

Post-traumatic olfactory dysfunction (PTOD) is associated with a significant decrease in quality of life. The present study aimed to explore whether PTOD is associated with depression and changes in sexuality. There were two groups in this case–control study. The patient group consisted of patients with PTOD (n = 55), and the control group comprised healthy individuals without the olfactory disorder (n = 115). Olfactory function, depression, partnership, and sexual satisfaction were assessed using the Iranian version of the Sniffin’ Sticks test (Ir-SST), Beck Depression Inventory (BDI), Enrich Couple Scale (ECS) and Sexual Satisfaction Scale for Women (SSSW). The BDI scores were higher in the patient group than in the control group (p < 0.001). The SSSW score was lower in the patient group than in controls (p < 0.01), although the ECS score was not significantly different between patients and controls. Also, there was no significant difference in the severity of trauma between marital satisfaction and sexual satisfaction. However, the analysis showed a statistically significant difference in depression scores in connection with the head trauma severity. In the PTOD group, depression was increased and sexual satisfaction declined. Understanding the association of olfactory dysfunction with depression and sexuality allows patients and doctors to deal with less notable consequences of this disorder.
Protein co-expression network-based profiles revealed from laser-microdissected cancerous cells of lung squamous-cell carcinomas
Authors: Nishimura Toshihide , Fujii Kiyonaga , Nakamura Haruhiko , Naruki Saeko , Sakai Hiroki , Kimura Hiroyuki , Miyazawa Tomoyuki , Takagi Masayuki , Furuya Naoki , Marko-Varga Gyorgy , Kato Harubumi , Saji Hisashi
Published: October 2021

No therapeutic targets have been identified for lung squamous cell cancer (SqCC) which is the second most prevalent lung cancer because its molecular profiles remain unclear. This study aimed to unveil disease-related protein networks by proteomic and bioinformatic assessment of laser-microdissected cancerous cells from seven SqCCs compared with eight representative lung adenocarcinomas. We identified three network modules significant to lung SqCC using weighted gene co-expression network analysis. One module was intrinsically annotated to keratinization and cell proliferation of SqCC, accompanied by hypoxia-induced aerobic glycolysis, in which key regulators were activated (HIF1A, ROCK2, EFNA1-5) and highly suppressed (KMT2D). The other two modules were significant for translational initiation, nonsense-mediated mRNA decay, inhibited cell death, and interestingly, eIF2 signaling, in which key regulators, MYC and MLXIPL, were highly activated. Another key regulator LARP1, the master regulator in cap-dependent translation, was highly suppressed although upregulations were observed for hub proteins including EIF3F and LARP1 targeted ribosomal proteins, among which PS25 is the key ribosomal protein in IRES-dependent translation. Our results suggest an underlying progression mechanism largely caused by switching to the cap-independent, IRES-dependent translation of mRNA subsets encoding oncogenic proteins. Our findings may help to develop therapeutic strategies to improve patient outcomes.
Severe sudden sensorineural hearing loss related to risk of stroke and atherosclerosis
Authors: Tsuzuki Nobuyoshi , Wasano Koichiro , Oishi Naoki , Hentona Ko , Shimanuki Marie , Nishiyama Takanori , Hiraga Yoshihiko , Shinden Seiichi , Ogawa Kaoru
Published: October 2021

The cause of idiopathic sudden sensorineural hearing loss (idiopathic SSNHL)—diagnosed after excluding other causes of hearing loss, such as SSNHL associated with vestibular schwannoma (VS)—is unknown. The presumed pathogenesis of idiopathic SSNHL includes circulatory disorders (e.g., cochlear infarction). We tested the hypothesis that patients with SSNHL who are at high stroke risk will have a lower rate of VS compared to those with low stroke risk. The rationale is that the primary cause of SSNHL in patients with high stroke risk might be a circulatory disturbance. We conducted a retrospective study in six hospitals. Our sampling of SSNHL patients included those diagnosed with idiopathic SSNHL and VS-associated SSNHL. SSNHL patients who had a head MRI were stratified by severity of hearing loss and evaluated for differences in the detection rate of VS between the high-scoring CHADS2 (CHADS2-H-), an index of stroke risk, and low-scoring CHADS2 (CHADS2-L-) groups. We identified 916 patients who met the inclusion criteria. For severe hearing loss, the CHADS2-H group had a significantly lower rate of VS than the CHADS2-L group (OR 0 [95% CI 0.00–0.612]; P = 0.007). These results indirectly support the hypothesis that a primary cause of severe idiopathic SSNHL in those at high risk of stroke might be a circulatory disorder.
Microbiota and transcriptome changes of Culex pipiens pallens larvae exposed to Bacillus thuringiensis israelensis
Authors: Zhang Ruiling , Liu Wenjuan , Zhang Qian , Zhang Xinyu , Zhang Zhong
Published: October 2021

Culex pipiens pallens is an important vector of lymphatic filariasis and epidemic encephalitis. Mosquito control is the main strategy used for the prevention of mosquito-borne diseases. Bacillus thuringiensis israelensis (Bti) is an entomopathogenic bacterium widely used in mosquito control. In this study, we profiled the microbiota and transcriptional response of the larvae of Cx. pipiens pallens exposed to different concentrations of Bti. The results demonstrated that Bti induced a significant effect on both the microbiota and gene expression of Cx. pipiens pallens. Compared to the control group, the predominant bacteria changed from Actinobacteria to Firmicutes, and with increase in the concentration of Bti, the abundance of Actinobacteria was gradually reduced. Similar changes were also detected at the genus level, where Bacillus replaced Microbacterium, becoming the predominant genus in Bti-exposed groups. Furthermore, alpha diversity analysis indicated that Bti exposure changed the diversity of the microbota, possibly because the dysbiosis caused by the Bti infection inhibits some bacteria and provides opportunities to other opportunistic taxa. Pathway analysis revealed significant enhancement for processes associated with sphingolipid metabolism, glutathione metabolism and glycerophospholipid metabolism between all Bti-exposed groups and control group. Additionally, genes associated with the Toll and Imd signaling pathway were found to be notably upregulated. Bti infection significantly changed the bacterial community of larvae of Cx. pipiens pallens.
Detection of nanoparticles suspended in a light scattering medium
Authors: Ye Yan , Pui David Y. H.
Published: October 2021

Intentionally intensifying the light scattering of medium molecules can allow the detection of suspended nanoparticles under conditions not suitable for conventional optical microscopies or laser particle counters. Here, we demonstrate how the collective light scattering of medium molecules and nanoparticles is imaged in response to the power, frequency, and oscillating direction of the incident light wave electric field, and how this response can be used to distinguish between nanoparticles and microparticles, such as viruses or bacteria. Under conditions that the medium light scattering is intensified, suspended nanoparticles appear as magnified shiny moving dots superimposed on the quasi-steady background of medium light scattering. Utilizing the visual enlargement resulted from the enhanced light scattering and possible light interference, we can detect directly suspended nanoparticles that are much smaller than visible light wavelengths even in unopened water bottles or other large containers. This suggests new approaches for detecting nanoparticles with many potential applications.
Reduced risk of skin cancer and internal malignancies in vitiligo patients: a retrospective population-based cohort study in Taiwan
Authors: Weng Yu-Ching , Ho Hsiu J. , Chang Yi-Ling , Chang Yun-Ting , Wu Chun-Ying , Chen Yi-Ju
Published: October 2021

The relationship between cancer and vitiligo has been explored but with inconsistent results. To examine the long-term cancer risk in vitiligo patients, we conducted a retrospective nationwide cohort study. From the National Health Insurance Research Database of Taiwan, a total of 13,824 vitiligo patients were identified and matched with 55,296 reference subjects without vitiligo by age, gender, and propensity score estimated by major comorbidities from 1997 to 2013. Demographic characteristics and comorbidities were compared between these two groups. Incidence rate ratios and hazard ratios (HRs) were calculated to examine cancer risks. The 16-year incidence rates of overall cancers were 621.06 (566.56–675.55) and 726.99 (697.24–756.74) per 100,000 person-years in the vitiligo and reference groups. Patients with vitiligo showed a significantly decreased risk of overall cancers [adjusted HR, 0.85; 95% confidence interval (CI), 0.77 to 0.93,p < 0.001] compared with reference subjects without vitiligo after adjusting for age, sex, comorbidities, and treatments. The risks of basal cell carcinoma (BCC) and squamous cell carcinoma (SCC) were significantly reduced (adjusted HR 0.21, 95% CI 0.11–0.38,p < 0.001), as well as internal malignancies (adjusted HR 0.89, 95% CI 0.81–0.99,p = 0.026). The results were consistent across different subgroups of patients, including male gender, ages more than 40 years, and those receiving long-term systemic disease-modifying antirheumatic drugs and phototherapies. Information related to phenotype, disease duration, vitiligo lesion sites, family history of vitiligo or cancer, occupation, and personal lifestyle was not included in the database. Vitiligo is associated with reduced risks of BCC and SCC, as well as internal malignancies.
A novel cardiovascular magnetic resonance risk score for predicting mortality following surgical aortic valve replacement
Authors: Vassiliou Vassilios S. , Pavlou Menelaos , Malley Tamir , Halliday Brian P. , Tsampasian Vasiliki , Raphael Claire E. , Tse Gary , Vieira Miguel Silva , Auger Dominique , Everett Russell , Chin Calvin , Alpendurada Francisco , Pepper John , Pennell Dudley J. , Newby David E. , Jabbour Andrew , Dweck Marc R. , Prasad Sanjay K.
Published: October 2021

The increasing prevalence of patients with aortic stenosis worldwide highlights a clinical need for improved and accurate prediction of clinical outcomes following surgery. We investigated patient demographic and cardiovascular magnetic resonance (CMR) characteristics to formulate a dedicated risk score estimating long-term survival following surgery. We recruited consecutive patients undergoing CMR with gadolinium administration prior to surgical aortic valve replacement from 2003 to 2016 in two UK centres. The outcome was overall mortality. A total of 250 patients were included (68 ± 12 years, male 185 (60%), with pre-operative mean aortic valve area 0.93 ± 0.32cm2, LVEF 62 ± 17%) and followed for 6.0 ± 3.3 years. Sixty-one deaths occurred, with 10-year mortality of 23.6%. Multivariable analysis showed that increasing age (HR 1.04, P = 0.005), use of antiplatelet therapy (HR 0.54, P = 0.027), presence of infarction or midwall late gadolinium enhancement (HR 1.52 and HR 2.14 respectively, combined P = 0.12), higher indexed left ventricular stroke volume (HR 0.98, P = 0.043) and higher left atrial ejection fraction (HR 0.98, P = 0.083) associated with mortality and developed a risk score with good discrimination. This is the first dedicated risk prediction score for patients with aortic stenosis undergoing surgical aortic valve replacement providing an individualised estimate for overall mortality. This model can help clinicians individualising medical and surgical care.

Trial Registration ClinicalTrials.gov Identifier: NCT00930735 and ClinicalTrials.gov Identifier: NCT01755936.