bluuebunny/crossref_metadata_2025 huggingface dataset:
The external jugular vein is a feasible and safe alternative access for retrieval of inferior vena cava filter
Authors: Zeng Xiong , Zeng Xiande , Zeng Qingfu , Wu Yuewu , Zhang Shanzhong , Yang Yujin , Zhu Xianhua , Zhang Wenwen , Xu Yingqi , Min Xixi , Chen Wei , Zhou Weimin , Qiu Jiehua
Published: February 2022

Purpose:

The purpose is to analyze whether the external jugular vein (EJV) is a feasible and safe alternative access for the retrieval IVCFs designed for the jugular approach.

Methods:

This study was designed as a nonrandomized, controlled study. The patients were divided into two groups: the IJV or EJV access groups. All operations were performed by the vascular surgery team. The main outcome was the technical success rate. The secondary outcomes included (1) the IVCF retrieval rate; (2) the time required to puncture the access vein (min); (3) the number of punctures required for access, and other aspects.

Results:

A total of 119 patients were recruited for IVCF retrieval. Seventeen patients refused to join this trial, leaving 58 patients in the IJV group and 44 patients in the EJV group. In the IJV group, technical success was not achieved in one patient who started in the EJV group and was transferred to the IJV group. There was no significant difference in age, comorbidities, or technical success rate between the two groups. Significant differences were observed in puncture time (min), number of punctures, and inadvertent puncture of the carotid artery. All of the patients were discharged 1 or 2 days after the operation.

Conclusion:

EJV is safe and feasible alternative access for the retrieval of IVCFs that are designed for jugular approaches.
Research on the reconstruction configuration and motion behavior of unmanned metamorphic vehicle
Authors: Liu Jun , Yang Pengliang , Lu Mingming , Sun Lei , Huang He
Published: February 2022

As a new type of ground transportation system, an unmanned metamorphic vehicle can naturally switch between wheeled driving and legged walking through vehicle reconstruction. Therefore, compared with traditional vehicles, unmanned metamorphic vehicles can move quickly on structural roads and have high trafficability on nonstructural roads. Furthermore, an unmanned metamorphic vehicle is applicable to interstellar detection and earthquake rescue. In this study, the topological structure of an unmanned metamorphic vehicle was designed and the reconstruction process was described. In addition, a kinematic analysis was conducted on the three stages (supporting, lifting, and standing-up) of reconfiguring the metamorphic vehicle. The kinematic and corresponding dynamic models were established based on the screw theory and the Lagrange equation. A Matlab/Simulink simulation was then carried out for the unmanned metamorphic vehicle. The simulation results describe the motion behavior of the system reconstruction, illustrating that the established kinematic and dynamic models are reasonable. Finally, the correctness of the abovementioned kinematic and dynamic models was further verified by Adams virtual prototype simulation. The above findings provide a solid foundation for future studies on stability control during the reconstruction of unmanned metamorphic vehicles.
A new megastigmane, known porphyrinic and galloylated bioactive derivatives from the leaves of Gymnosporia senegalensis
Authors: Tatsimo Ndendoung Simplice Jöel , Tamokou Jean-De-Dieu , Toume Kazufumi , Havyarimana Léopold , Ekom Steve Endeguele , Komatsu Katsuko
Published: February 2022

Investigation of the non-polar (rich in chlorophyll) and polar fractions of the ethyl acetate extract of Gymnosporia senegalensis (Lam.) Loes. leaves led to the isolation of nine compounds, including one new megastigmane derivative, (3 R*,5 S*,6 R*,7 E,9ξ)-7-megastigmene-3,6,9-triol-3- O-β-D-(6′- O-galloyl)glucopyranoside (1), four known galloylated derivatives (2, 3, 8, 9), three known chlorophyll derivatives (4–6), and one known fatty acid (7). The structures of the isolates were determined by means of spectroscopic and spectrometric data, as well as by comparison with literature values. The extracts and isolated compounds were tested for their antibacterial, antifungal, and antioxidant activities. The isolates showed weak to high antimicrobial activities with phaeophytin A (4) being the most active one whereas phaeophorbide-a (5) and Chlorine e6 trimethyl ester (6) exhibited the highest antioxidant activities.
Synthesis of aluminum alkylphosphinates under atmospheric pressure
Authors: Zhang Mengting , Shi Shengbin , Lei Yan , Gu Liuyu , Gao Lijing , Xiao Guomin
Published: February 2022

Alkylphosphinates have received extensive attention in the past few decades because of their very useful mechanical properties, electrical properties, low density, and low toxicity, and have been widely used in flame-retardant materials and other fields. In this work, aluminum diethylphosphinate is successfully synthesized under atmospheric pressure. More importantly, two novel dialkylphosphinates, aluminum dioctylphosphinate and aluminum didecylphosphinate, are first synthesized and characterized. The structures of these aluminum dialkylphosphinates are confirmed by nuclear magnetic resonance, Fourier-transform infrared spectroscopy, and high-resolution mass spectrometry, and the microscopic morphology and thermal stability are analyzed by scanning electron microscopy and thermogravimetric analysis, respectively. Furthermore, conditions for the synthesis of aluminum dioctylphosphinate are optimized. Compared with the traditional method of synthesizing dialkylphosphinates under high pressure, the method reported in this paper has the advantages of high safety, easy operation, and low economic cost, which makes it suitable for industrial production.
New functionalisation reactions of graphitic carbon nitrides: Computational and experimental studies
Authors: Parkes Ellen , Lisowska Karolina , McMillan Paul F , Corà Furio , Clancy Adam J
Published: February 2022

The functionalisation of two-dimensional materials is key to modify their properties and facilitate assembly into functional devices. Here, new reactions have been proposed to modify crystalline two-dimensional carbon nitrides of polytriazine imide structure. Both amine alkylation and aryl-nitrene-based reactions have been explored computationally and with exploratory synthetic trials. The approach illustrates that alkylation is unfavourable, particularly at basal-plane sites. In contrast, while initial trial reactions were inconclusive, the radical-addition of nitrenes is shown to be energetically favourable, with a preference for functionalising sheet edges to minimise steric effects.
Introducing A Day-Case Shoulder Arthroplasty Pathway In The UK – How We Did It
Authors: Allen James , Abdelmonem Mohamed , Fieraru Gabriel , Guyver Paul
Published: February 2022

Background

As the demand for elective orthopaedics grows, day-case arthroplasty is gaining popularity. The aim of this study was to create a safe and reproducible pathway for day-case shoulder arthroplasty (DCSA) based upon a literature review and discussion with the local multidisciplinary team (MDT).

Methods

A literature review was performed using OVID MEDLINE and Embase databases reporting 90-day complication and admission rates following DCSA. Minimum follow-up was 30 days. Day-case was defined as discharge on the same day of surgery.

Results

The literature review revealed a mean 90-day complication rate of 7.7% [range, 0–15.9%] and mean 90-day readmission rate of 2.5% [range 0−9.3%]. A pilot protocol was devised based upon the literature review and consisted of 5 phases: (1) pre-operative assessment, (2) intra-operative phase, (3) post-operative phase, (4) follow-up, and (5) readmission protocol. This was presented, discussed, amended, and ultimately ratified by the local MDT. In May 2021 the unit successfully completed its first day-case shoulder arthroplasty.

Discussion

This study proposes a safe and reproducible pathway for DCSA. Patient selection, well-defined protocols and communication within the MDT are important factors to achieve this. Further studies with extended follow-up will be needed to gauge long-term success within our unit.
The Development of Shyness from Late Childhood to Adolescence: A Longitudinal Study of Mexican-Origin Youth
Authors: Lawson Katherine M. , Barrett Brenna L. , Cerny Ryan J. , Enrici Kaitlyn E. , Garcia-Cardenas Juan Eduardo , Gonzales Catherine E. , Hernandez Isidro D. , Iacobacci Carrina P. , Lin Tiffanie , Martinez Urieta Nancy Y. , Moreno Patricia , Rivera Marissa G. , Teichrow Devin J. , Vizcarra Anabel , Hostinar Camelia E. , Robins Richard W.
Published: February 2022

Shyness, the tendency to be inhibited and uncomfortable in novel social situations, is a consequential personality trait, especially during adolescence. The present study examined the development of shyness from late childhood (age 10) through adolescence (age 16) using data from a large, longitudinal study of Mexican-origin youth ( N = 674). Using both self- and mother-reports of shyness assessed via the Early Adolescent Temperament Questionnaire–Revised, we found moderate to high rank-order stabilities across 2-year intervals and a mean-level decrease in shyness from age 10 to 16. Anxiety and depression were associated with higher initial levels of shyness, and anxiety was associated with greater decreases in shyness from age 10 to 16. Contrary to predictions, neither nativity (country of birth) nor language proficiency (English, Spanish) was associated with the development of shyness across adolescence. Thus, youth generally decline in shyness during adolescence, although there is substantial individual variability in shyness trajectories.
Effecting Change in Teachers’ Epistemological and Pedagogical Beliefs about Vocabulary Learning and Teaching: The Role of Dialogic Reflection
Authors: Chung Edsoulla
Published: February 2022

Despite its significance, the relationship between changes in teachers’ beliefs regarding specific aspects of language learning and teaching, and the developmental processes of such beliefs in relation to teacher reflection has remained under-researched. Accordingly, this paper reports the results of a case study that examined how two English language teachers who engaged in dialogic reflection on vocabulary learning and teaching experienced changes in their beliefs. Data collected from in-depth semi-structured interviews, teachers’ professional dialogues and reflective journals confirm the role of dialogic reflection in facilitating professional learning; reveal the diverse development processes of teachers’ epistemological and pedagogical beliefs; and highlight the complicated relationship between knowledge enhancement and belief change. Based on insights gleaned from the study, this paper provides practical suggestions for teacher development and directions for further research.
The life history theory of the Lord of the Rings: a randomized controlled trial of using fact versus fiction to teach life history theory
Authors: Maley Carlo C. , Seyedi Sareh
Published: February 2022

Does asking students to apply concepts from evolution to a fictional context, compared to a novel biological context, improve their understanding, exam performance or enjoyment of the material? Or does it harm their education by taking time away from true biology? At our institution, we sometimes ask students to apply life history theory to species from fictional movies, television shows or books. Previously, we had used a factual article on life history theory, to supplement our textbook. We wrote an alternative introduction to life history theory (included in the additional files for educational use), using Tolkien’s fictional species from his Lord of the Rings books. We also introduce the biological species definition, sexual selection, sexual dimorphism, kin selection, and the handicap principle, as those concepts arose naturally in the discussion of the fictional species. Life history theory predicts strong correlations between traits affecting reproduction, growth and survival, which are all shaped by the ecology of the species. Thus, we can teach life history theory by asking students to infer traits and aspects of the ecology of a fictional species that have never been described, based on the partial information included in the fictional sources. In a large, third year undergraduate evolution course at Arizona State University, we randomized 16 tutorial sections of a total of 264 students to either read our article on the life history theory of Lord of the Rings, or the factual article we had used previously in the course. We found that the exam performance on life history questions for the two groups were almost identical, except that fans of The Lord of the Rings who had read our article did better on the exam. Enjoyment, engagement and interest in life history theory was approximately a full point higher on a 5-point Likert scale for the students that had read the fictional article, and was highly statistically significantly different (T-test p < 0.001 for all questions). There was no difference between the two groups in their familiarity or enjoyment of The Lord of the Rings stories themselves. Reading the article that taught life history theory by applying it to the species of The Lord of the Rings neither helped nor harmed exam performance, but did significantly improve student enjoyment, engagement and interest in life history theory, and even improved exam scores in students who liked The Lord of the Rings. Using fiction to teach science may also help to engage non-traditional students, such as world-builders, outside of our institutions of education. By encouraging students to apply the scientific ideas to their favorite stories from their own cultures, we may be able to improve both inclusivity and education.
Critical evaluation of the microbial turnover to biomass approach for the estimation of biogenic non-extractable residues (NER)
Authors: Trapp Stefan , Brock Andreas Libonati , Kästner Matthias , Schäffer Andreas , Hennecke Dieter
Published: February 2022

Background

Persistence is a key criterion for the risk assessment of chemicals. In degradation tests, microbial biodegradation of labeled test chemicals leads to the incorporation of the label in microbial biomass, resulting in biogenic non-extractable residues (bioNER), which are not considered as harmful in persistence assessment. The amount of bioNER can be estimated using the microbial turnover to biomass (MTB) model. MTB estimates the biomass growth during productive degradation of a compound from theoretical growth yield and CO2-formation and gives an upper and a lower value for bioNER formation. The aim of this study is use available experimental data for bioNER to assess the validity, accuracy and precision of the MTB method as new tool in persistence assessment.

Results

We collected experimental data in order to test accuracy and precision of this estimation method. In total, 16 experimental studies were found in literature where bioNER was experimentally quantified. Hereof, 13 studies used the amount of label recovered from total amino acid (tAA) content as proxy for bioNER. Unfortunately, the comparison with experimental data was difficult due to the variety of employed methods. A conversion factor is required to extrapolate from tAA on bioNER, and this factor may vary during the experiment and between experiments. The bioNER formation for all compounds tested was calculated with the MTB method, and the outcome was compared to measured tAA as proxy for bioNER. The relation between predicted and measured bioNER was significant, but no better correlation was obtained than with CO2 to tAA. The mean absolute error of the prediction (low MTB versus tAA) was 5% applied label (range 0.3 to 16%). Some deviation between measured results and calculated bioNER could be contributed to uncertainties in the experimental determination, as shown by variance in replicates (bromoxynil) or high background of label in sterile samples (sulfadiazine).

Conclusions

MTB thus provides a robust model for determining of the potential amounts of biomass and bioNER formed from the degradation of organic chemicals.
CROPSR: an automated platform for complex genome-wide CRISPR gRNA design and validation
Authors: Müller Paul Hans , Istanto Dave D. , Heldenbrand Jacob , Hudson Matthew E.
Published: February 2022

Background

CRISPR/Cas9 technology has become an important tool to generate targeted, highly specific genome mutations. The technology has great potential for crop improvement, as crop genomes are tailored to optimize specific traits over generations of breeding. Many crops have highly complex and polyploid genomes, particularly those used for bioenergy or bioproducts. The majority of tools currently available for designing and evaluating gRNAs for CRISPR experiments were developed based on mammalian genomes that do not share the characteristics or design criteria for crop genomes.

Results

We have developed an open source tool for genome-wide design and evaluation of gRNA sequences for CRISPR experiments, CROPSR. The genome-wide approach provides a significant decrease in the time required to design a CRISPR experiment, including validation through PCR, at the expense of an overhead compute time required once per genome, at the first run. To better cater to the needs of crop geneticists, restrictions imposed by other packages on design and evaluation of gRNA sequences were lifted. A new machine learning model was developed to provide scores while avoiding situations in which the currently available tools sometimes failed to provide guides for repetitive, A/T-rich genomic regions. We show that our gRNA scoring model provides a significant increase in prediction accuracy over existing tools, even in non-crop genomes.

Conclusions

CROPSR provides the scientific community with new methods and a new workflow for performing CRISPR/Cas9 knockout experiments. CROPSR reduces the challenges of working in crops, and helps speed gRNA sequence design, evaluation and validation. We hope that the new software will accelerate discovery and reduce the number of failed experiments.
Genetic effect of an InDel in the promoter region of the NUDT15 and its effect on myoblast proliferation in chickens
Authors: Wei Chengjie , Niu Yufang , Chen Bingjie , Qin Panpan , Wang Yanxing , Hou Dan , Li Tong , Li Ruiting , Wang Chunxiu , Yin Huadong , Han Ruili , Xu Huifen , Tian Yadong , Liu Xiaojun , Kang Xiangtao , Li Zhuanjian
Published: February 2022

Background

Molecular breeding accelerates the speed of animal breeding. Screening molecular markers that can affect economic traits through genome-wide association studies (GWAS) can provide a theoretical basis for molecular breeding. At present, a large number of molecular markers have been screened in poultry research, but few reports on how molecular markers affect economic traits exist. It is particularly important to reveal the action mechanisms of molecular markers, which can provide more accurate information for molecular breeding.

Results

The aim of this study was to investigate the relationships between two indels (NUDT15-indel-2777 and NUDT15-indel-1673) in the promoter region of NUDT15 and growth and carcass traits in chickens and to explore the regulatory mechanism of NUDT15. Significant differences were found in genotype and allele frequencies among commercial broilers, commercial laying hens and dual-purpose chickens. The results of association analyses showed that these two indel loci could significantly affect growth traits, such as body weight, and carcass traits. Tissue expression profiling at E12 showed that the expression of NUDT15 was significantly higher in skeletal muscle, and time-expression profiling of leg muscle showed that the expression of NUDT15 in myoblasts was significantly higher in the E10 and E12 proliferation stages than in other stages. Promoter activity analysis showed that pro-1673-I and pro-1673-D significantly inhibited promoter activity, and the promoter activity of pro-1673-D was significantly lower than that of pro-1673-I. In addition, when NUDT15 was overexpressed or underwent interference in chicken primary myoblasts (CPMs), NUDT15 could inhibit the proliferation of CPMs.

Conclusion

The results suggest that the studied indels in the promoter region of NUDT15 may regulate the proliferation of CPMs by affecting NUDT15 expression, ultimately affecting the growth and carcass traits of chickens. These indel polymorphisms may be used together as molecular markers for improving economic traits in chickens.
Inverse probability weighting to handle attrition in cohort studies: some guidance and a call for caution
Authors: Metten Marie-Astrid , Costet Nathalie , Multigner Luc , Viel Jean-François , Chauvet Guillaume
Published: February 2022

Background

Attrition in cohort studies challenges causal inference. Although inverse probability weighting (IPW) has been proposed to handle attrition in association analyses, its relevance has been little studied in this context. We aimed to investigate its ability to correct for selection bias in exposure-outcome estimation by addressing an important methodological issue: the specification of the response model.

Methods

A simulation study compared the IPW method with complete-case analysis (CCA) for nine response-mechanism scenarios (3 missing at random – MAR and 6 missing not at random - MNAR). Eighteen response models differing by the type of variables included were assessed.

Results

The IPW method was equivalent to CCA in terms of bias and consistently less efficient in all scenarios, regardless of the response model tested. The most effective response model included only the confounding factors of the association model.

Conclusion

Our study questions the ability of the IPW method to correct for selection bias in situations of attrition leading to missing outcomes. If the method is to be used, we encourage including only the confounding variables of the association of interest in the response model.
Full recovery after prolonged resuscitation in a pediatric patient due to fulminant myocarditis: a case report with three-year follow-up
Authors: Gu Yanping , Xue Peng , Chen Hong-Lin , Zou Guojin , Ni Yongcheng , Li Lin , Lu Lijuan , Chen Hao , Zheng Aibin
Published: February 2022

Background

Fulminant myocarditis (FM) is a common life-threatening disease in pediatric patients, which can result in sudden cardiac arrest (CA). Whether prolonged cardiopulmonary resuscitation (CPR) is beneficial to FM induced CA is unknown.

Case presentation

We reported the case of an 8-year-old child with FM. At 14:49 of the day after admission, the ECG monitoring indicated ventricular flutter. The patient was immediately given continuous external cardiac compression. Electric cardioversion (energy 30J) and electric defibrillation (energy 50 J, 100 J, 100 J) were given. Continuous chest compression was conducted until extracorporeal membrane oxygenation (ECMO) successfully placed at 19:30 P.M. The total duration of CPR was 291 min. Nine days later, the ECMO was removed; and 29 days later, the patient was discharged from hospital. In the three years of follow-up, the boy showed a full recovery without neurological sequela. At present, his daily activities have returned to normal and his academic performance at school is excellent.

Conclusions

Prolonged CPR can be used in FM induced in-hospital CA in pediatric patients, especially during preparation for ECMO after the failure of standard resuscitation measures.
Marker- three dimensional measurement versus traditional radiographic measurement in the treatment of tibial fracture using Taylor spatial frame
Authors: Liu Qixin , Liu Yanshi , Li Hong , Fu Xuefei , Zhang Xingpeng , Liu Sida , Zhang Jinli , Zhang Tao
Published: February 2022

Background

The Taylor Spatial Frame (TSF) has been widely used for tibial fracture. However, traditional radiographic measurement method is complicated and the reduction accuracy is affected by various factors. The purpose of this study was to propose a new marker- three dimensional (3D) measurement method and determine the differences of reduction outcomes, if any, between marker-3D measurement method and traditional radiographic measurement in the TSF treatment.

Methods

Forty-one patients with tibial fracture treated by TSF in our institution were retrospectively analyzed from January 2016 to June 2019, including 21 patients in the marker-3D measurement group (experimental group) and 20 patients in the traditional radiographic measurement group (control group). In the experimental group, 3D reconstruction with 6 markers installed on the TSF was performed to determine the electronic prescription. In the control group, the anteroposterior (AP) and lateral radiographs were performed for the traditional parameter measurements. The effectiveness was evaluated by the residual displacement deformity (RDD) and residual angle deformity (RAD) in the coronal and sagittal plane, according to the AP and lateral X-rays after reduction.

Results

All patients achieved functional reduction. The residual RDD in AP view was 0.5 (0, 1.72) mm in experimental group and 1.74 (0.43, 3.67) mm in control group. The residual RAD in AP view was 0 (0, 1.25) ° in experimental group and 1.25 (0.62, 1.95) °in control group. As for the lateral view, the RDD was 0 (0, 1.22) mm in experimental group and 2.02 (0, 3.74) mm in control group, the RAD was 0 (0, 0) ° in experimental group and 1.42 (0, 1.93) ° in control group. Significant differences in all above comparisons were observed between the two groups (AP view RDD: P = 0.024, RAD: P = 0.020; Lateral view RDD: P = 0.016, RAD: P = 0.004).

Conclusions

The present study introduced a marker-3D measurement method to complement the current TSF treatment. This method avoids the manual measurement error and improves the accuracy of fracture reduction, providing potential advantages of bone healing and function rehabilitation.
Serum ferritin and incident cardiometabolic diseases in Scottish adults
Authors: Suárez-Ortegón Milton-Fabian , McLachlan Stela , Fernandez-Real José-Manuel , Tuomainen Tomi-Pekka , Aregbesola Alex , Wild Sarah H.
Published: February 2022

Background

Iron stores, estimated as ferritin levels, and type 2 diabetes (T2D) have been associated previously, while findings regarding coronary heart disease (CHD) and cerebrovascular disease (CEVD) are still inconclusive. No study has focused on simultaneous evaluation of associations between iron stores and the above cardiometabolic diseases (CMD) in the same population. We aim to evaluate the association between serum ferritin and risk of T2D, CHD and CEVD in Scottish population over a wide range of ferritin levels.

Methods

Longitudinal study in 6,497 participants of the 1995 and 1998 Scottish health surveys, who were followed-up until 2011. Cox regression models were conducted adjusting for age, sex/menopausal status, fibrinogen, GGT levels, smoking, alcohol consumption, total cholesterol, HDL-cholesterol, blood pressure, and BMI. Ferritin was used as continuous (sex/menopausal status-specific Z score) and categorical variable (sex/menopausal status-specific quartiles, quintiles and sextiles).

Results

During follow-up, 4.9% of the participants developed T2D, 5.3% CHD, and 2.3% CEVD. By using ferritin quartiles, serum ferritin was positively associated with T2D, CHD and CEVD but only the association with T2D remained after adjustment for covariates [Quartile 4 v. 1: adjusted HR 95% CI 1.59 (1.10–2.34); P = 0.006]. When ferritin sextiles were used (6 v. 1), the ferritin-CEVD association became slightly stronger and significant [adjusted HR 95% CI 2.08 (1.09–3.94); P = 0.024].

Conclusions

Iron stores relate differently to each CMD. Serum ferritin levels were positively and independently associated with incident T2D, and with incident CEVD if higher cut-off points for high ferritin levels were considered.
Exploring social determinants of health in a Saudi Arabian primary health care setting: the need for a multidisciplinary approach
Authors: Almujadidi Basmah , Adams Alayne , Alquaiz Aljohara , Van Gurp Gerald , Schuster Tibor , Andermann Anne
Published: February 2022

Background

Action on social determinants of health (SDH) in primary health care settings is constrained by practitioners, organizational, and contextual factors. The aim of this study is to identify barriers and enablers for addressing SDH in clinical settings in Saudi Arabia, taking into consideration the influence of local cultural and social norms, to improve care and support for marginalized and underserved patients.

Methods

We conducted a qualitative study involving individual in-depth interviews with a sample of 17 primary health care physicians purposefully selected based on the inclusion criteria, as well as a focus group with four social workers, all recruited from King Khalid University Hospital (KKUH) in Riyadh, Saudi Arabia. All interviews were audio-recorded, translated from Arabic to English, transcribed verbatim, and analyzed using thematic analysis following a deductive-inductive approach.

Results

According to study participants, financial burdens, challenges in familial dynamics, mental health issues and aging population difficulties were common social problems in Saudi primary health care. Action on SDH in primary care was hindered by 1) lack of physician knowledge or training; 2) organizational barriers including time constraints, patient referral/follow up; 3) patient cultural norms and 4) lack of awareness of physician’s role in managing SDH. Enablers to more socially accountable care suggested by participants includes: 1) more education and training on addressing SDH in clinical care; 2) organizational innovations to streamline identification of SDH during patient encounters (e.g. case finding questionnaire completed in waiting room); 3) better interprofessional coordination and clarification of roles (e.g. when to refer to social work, what support is provided by physicians); 4) identifying opportunities for broader advocacy to improve living conditions for marginalized groups.

Conclusion

Enabling more socially accountable care requires a multipronged approach including leadership from the Ministry of Health, hospital administrations and medical schools. In particular, there is a need for: 1) training physicians to help patients in navigating social challenges; 2) improving clinical/administrative interprofessional teams, 3) mobilizing local communities in addressing social challenges; and 4) advocating for intersectoral action to prevent health inequities before they become more complex issues presenting to clinical care.
Dopamine signaling modulates microglial NLRP3 inflammasome activation: implications for Parkinson’s disease
Authors: Pike Adrianne F. , Longhena Francesca , Faustini Gaia , van Eik Jean-Marc , Gombert Iris , Herrebout Maaike A. C. , Fayed Mona M. H. E. , Sandre Michele , Varanita Tatiana , Teunissen Charlotte E. , Hoozemans Jeroen J. M. , Bellucci Arianna , Veerhuis Robert , Bubacco Luigi
Published: February 2022

Background

Parkinson’s disease (PD) is characterized by the loss of nigral dopaminergic neurons leading to impaired striatal dopamine signaling, α-synuclein- (α-syn-) rich inclusions, and neuroinflammation. Degenerating neurons are surrounded by activated microglia with increased secretion of interleukin-1β (IL-1β), driven largely by the NLRP3 inflammasome. A critical role for microglial NLRP3 inflammasome activation in the progression of both dopaminergic neurodegeneration and α-syn pathology has been demonstrated in parkinsonism mouse models. Fibrillar α-syn activates this inflammasome in mouse and human macrophages, and we have shown previously that the same holds true for primary human microglia. Dopamine blocks microglial NLRP3 inflammasome activation in the MPTP model, but its effects in this framework, highly relevant to PD, remain unexplored in primary human microglia and in other in vivo parkinsonism models.

Methods

Biochemical techniques including quantification of IL-1β secretion and confocal microscopy were employed to gain insight into dopamine signaling-mediated inhibition of the NLRP3 inflammasome mechanism in primary human microglia and the SYN120 transgenic mouse model. Dopamine and related metabolites were applied to human microglia together with various inflammasome activating stimuli. The involvement of the receptors through which these catecholamines were predicted to act were assessed with agonists in both species.

Results

We show in primary human microglia that dopamine, l-DOPA, and high extracellular K+, but not norepinephrine and epinephrine, block canonical, non-canonical, and α-syn-mediated NLRP3 inflammasome-driven IL-1β secretion. This suggests that dopamine acts as an inflammasome inhibitor in human microglia. Accordingly, we provide evidence that dopamine exerts its inhibitory effect through dopamine receptor D1 and D2 (DRD1 and DRD2) signaling. We also show that aged mice transgenic for human C-terminally truncated (1–120) α-syn (SYN120 tg mice) display increased NLRP3 inflammasome activation in comparison to WT mice that is diminished upon DRD1 agonism.

Conclusions

Dopamine inhibits canonical, non-canonical, and α-syn-mediated activation of the NLRP3 inflammasome in primary human microglia, as does high extracellular K+. We suggest that dopamine serves as an endogenous repressor of the K+ efflux-dependent microglial NLRP3 inflammasome activation that contributes to dopaminergic neurodegeneration in PD, and that this reciprocation may account for the specific vulnerability of these neurons to disease pathology.
Structural and functional foot disorders in patients with genodermatoses: a single-centre, retrospective chart review
Authors: Pietrzak Aldona , Wawrzycki Bartlomiej , Schmuth Matthias , Wertheim-Tysarowska Katarzyna
Published: February 2022

Background

Skin lesions on the feet and foot deformities impair daily activities and decrease quality of life. Although substantial foot deformities occur in many genodermatoses, few reports have been published on this topic. Therefore, we performed a retrospective chart review to identify patients with genodermatoses and foot disorders. We included 16 patients, who were investigated clinically and with molecular biology.

Results

The following genodermatoses with foot deformities were detected: autosomal recessive congenital ichthyosis (ARCI, n = 7); palmoplantar keratodermas (PPKs, n = 6); ichthyosis follicularis, atrichia, and photophobia (IFAP, n = 1); ectrodactyly-ectodermal dysplasia-clefting (EEC, n = 1); and ichthyosis with confetti (IWC, n = 1). Foot problems not only varied in severity depending on the disease but also showed phenotypic heterogeneity among patients with the same condition. Foot deformities were most pronounced in patients with EEC (split foot) or IWC (contractures) and less severe in those with ARCI (clawed toes), IFAP (hollow feet), or PPK (no bone abnormalities in the feet).

Conclusion

Because a range of distinct genodermatoses involve foot abnormalities, early rehabilitation and other corrective measures should be provided to patients with foot involvement to improve gait and prevent/delay irreversible complications.
Change of hypothalamic adult neurogenesis in mice by chronic treatment of fluoxetine
Authors: Ohira Koji
Published: February 2022

Objective

More than half of patients with depression display eating disorders, such as bulimia nervosa and anorexia nervosa. Feeding centers are located in the hypothalamus, and hypothalamic adult neurogenesis has an important role in feeding and energy balance. Antidepressants, which can regulate adult neurogenesis in the hippocampus, olfactory bulb, and neocortex, are used for eating disorders, but it is unclear whether antidepressants change hypothalamic adult neurogenesis. In this study, we used immunohistological analysis to assess effects of the antidepressant fluoxetine (FLX) on hypothalamic adult neurogenesis of adult mice.

Results

Expressions of the proliferating cell marker, Ki67, and the neural stem cell marker, nestin, were significantly decreased in the hypothalamus by FLX. As regard to postmitotic cells, the number of the neural marker, NeuN, positive cells was significantly upregulated by FLX, but that of the astrocytic marker, S100B, positive cells was significantly reduced by FLX. The number of the oligodendrocyte marker, Olig2, positive cells was not changed by FLX. Interestingly, FLX treatment did not affect the total number of newly generated cells in the hypothalamus, comparing that in controls. These results suggest that FLX treatment influence hypothalamic adult neurogenesis and shift the balance between the numbers of neurons and astrocytes under studied conditions.
Sensitivity and specificity of a brief scale to evaluate psychological violence at work in Peruvian health professionals
Authors: Abregú Tueros Luis Fidel , dos Santos Rosa Roger
Published: February 2022

Objective

Most studies in Latin America that evaluate psychological violence at work (PVW) focus on measuring occurrences of PVW. However, the discriminative validity and randomness of instruments used for evaluating incidents of PVW that are generated by agents internal to the workplace in the Peruvian health sector have not yet been studied. The objective of this study was to determine the sensitivity and specificity of the Scale of Psychological Violence in Health Professionals (SVP-Health) in the Peruvian population. For this purpose, a cross-sectional study based on the two-stage administration of guided surveys and ROC (receiver operating characteristic) curve analysis was performed.

Results

The study included 188 professionals from ten care centres in Peru. The professionals were divided into two groups of 94 subjects: those who had experienced PVW and those who had not. The average age of the participants was 36.8 ± 10.5 years; their work experience ranged between one and 35 years; and 59% of the sample were women. According to an analysis based on the ROC curve, (a) there was a significant area under the curve (AUC = 0.899) with adequate randomness; and (b) the SPV-Health adequately distinguished subjects with PVW from those without PVW (89% versus 94%).
Metastatic mucoepidermoid carcinoma to the pleura: a case report
Authors: Mashiana Simran , Duarte Ernesto Martinez
Published: February 2022

Background

Mucoepidermoid carcinoma is the most common malignant neoplasm arising from the salivary glands (Ali et al. in J Ayub Med Coll Abbottabad 20(2): 141-2, 2008, Xi et al. in World J Surg Oncol 10: 232, 2012). When arising from anatomic sites other than the salivary glands it can be a diagnostic challenge. Primary and metastatic mucoepidermoid carcinoma from and to the pleura are extremely rare entities that are frequently misdiagnosed as adenocarcinoma, adenosquamous carcinoma, or squamous cell carcinoma (Xi et al. in World J Surg Oncol 10: 232, 2012).

Case presentation

We describe an unusual case of a 64-year-old Caucasian female patient with metastatic high-grade mucoepidermoid carcinoma to the pleura, morphologically resembling squamous cell carcinoma. Molecular studies of both the parotid gland and pleural tumors helped prove the metastatic nature of the pleural lesion.

Conclusions

 Metastatic mucoepidermoid carcinoma to the pleura is a rare entity, frequently misdiagnosed as squamous cell carcinoma. Differentiating between a lung primary and a metastatic disease has treatment implications and prognostic significance for the patient. When morphologic and immunophenotypic overlap exists, molecular testing can help distinguish mucoepidermoid carcinoma from other neoplasms.
Protocol for a systematic review of the in vivo studies on radiofrequency (100 kHz–300 GHz) electromagnetic field exposure and cancer
Authors: Pinto Rosanna , Ardoino Lucia , Giardullo Paola , Villani Paola , Marino Carmela
Published: February 2022

Background

An Italian project aims to review the scientific literature on the possible carcinogenicity of radiofrequency (100 kHz–300 GHz) electromagnetic field (RF-EMF) exposure. The ENEA team has to carry out a systematic review of the in vivo studies on this topic.

Objectives

Development of a protocol for a systematic review (meta-analysis included) to investigate the potential carcinogenic risk following RF-EMF in vivo exposure to doses above or within legal limits. The aims of this review are (1) to provide a descriptive and, if possible, a quantitative summary of the results of the examined RF-EMF in vivo studies, together with an assessment of the consistency of observations and of the causes of heterogeneity, and (2) to assess the weight of evidence to support or refute the hypothesis of carcinogenic effects caused by RF-EMF exposure and to draw conclusions about the potential for carcinogenicity of RF-EMF exposure.

Methods

We will search for relevant studies in electronic academic databases and in the reference list of selected papers and reviews on the topic, including the descriptive reviews on RF-EMF carcinogenic effect carried out by international panels of experts since 2011. The following elements of the PECO question were defined: experimental studies on rodents of both sexes, all ages and species, all genetic backgrounds (Population) exposed to RF-EMF alone, or in combination with other physical or chemical agents (Exposure); only studies reporting outcome data in exposed and sham control groups (Comparison); and all types of cancer with all tumor-related outcome measures (Outcome) will be included.

Only peer-reviewed articles written in English will be considered without limit in the publication date.

Eligibility criteria were defined for papers to be included. A risk of bias assessment will be performed using a tool specifically developed for animal studies. A meta-analysis will be performed, if feasible, for all outcome measures; for subgroup analysis, a minimum of 3 studies per subgroup will be required. If meta-analysis will not be possible, a narrative synthesis of the results will be reported.

Systematic review registration

PROSPERO CRD42020191105

Highlights

An Italian collaborative research agreement aims to review the scientific literature on the possible carcinogenicity of RF-EMF (100 kHz – 300 GHz).

The ENEA team will systematically review and, if possible, meta-analyse estimates the effects of in vivo exposure to RF-EMF exposure on cancer.

The ENEA group is a multidisciplinary team of researchers with a consolidated experience both in carcinogenicity experiments and radiofrequency dosimetric assessment.

The proposed protocol uses the NTP OHAT Approach for Systematic Review as an organizing framework.

The proposed protocol aims to lead to the first systematic review providing a strength of evidence assessment on this topic.
Prophylactic antimicrobials may not be needed to prevent surgical site infection after skin biopsy: a retrospective study
Authors: Akiyama Yuko , Norimatsu Yuta , Ohno Yuki
Published: February 2022

Background

Two types of skin biopsies are routinely performed in dermatology: excisional and punch biopsies. A punch biopsy is a relatively low-risk procedure for surgical site infections (SSIs) because of the shallow wound depth and short operative time. In Japan, prophylactic antimicrobial agents are often used after skin biopsies due to lack of consensus, and there is no mention of antimicrobial use after skin biopsies in Japanese guidelines. In this study, we investigated whether prophylactic antibiotic use after punch biopsies reduces the risk of SSI development.

Methods

Cases of punch biopsy performed in our dermatology department during a one-year period from April 2018 to March 2019 were included retrospectively. The cases were divided into a group with and another without prophylactic antimicrobial use after biopsy.

Results

A total of 75 cases of punch skin biopsy were reviewed. There were no cases of wound infection after punch biopsy in any of the groups. The number of years of experience of the physicians in the group that used antimicrobials was significantly higher than that in the group that did not use antimicrobials (P < 0.0001).

Conclusions

Our result suggests that the incidence of SSI in punch biopsies without prophylaxis seems to be low. However, further research is needed due to the small number of cases in this study.
Integrated meta-omics reveals new ruminal microbial features associated with feed efficiency in dairy cattle
Authors: Xue Ming-Yuan , Xie Yun-Yi , Zhong Yifan , Ma Xiao-Jiao , Sun Hui-Zeng , Liu Jian-Xin
Published: February 2022

Background

As the global population continues to grow, competition for resources between humans and livestock has been intensifying. Increasing milk protein production and improving feed efficiency are becoming increasingly important to meet the demand for high-quality dairy protein. In a previous study, we found that milk protein yield in dairy cows was associated with the rumen microbiome. The objective of this study was to elucidate the potential microbial features that underpins feed efficiency in dairy cows using metagenomics, metatranscriptomics, and metabolomics.

Results

Comparison of metagenomic and metatranscriptomic data revealed that the latter was a better approach to uncover the associations between rumen microbial functions and host performance. Co-occurrence network analysis of the rumen microbiome revealed differential microbial interaction patterns between the animals with different feed efficiency, with high-efficiency animals having more and stronger associations than low-efficiency animals. In the rumen of high-efficiency animals,Selenomonasand members of theSuccinivibrionaceaefamily positively interacted with each other, functioning as keystone members due to their essential ecological functions and active carbohydrate metabolic functions. At the metabolic level, analysis using random forest machine learning suggested that six ruminal metabolites (all derived from carbohydrates) could be used as metabolic markers that can potentially differentiate efficient and inefficient microbiomes, with an accuracy of prediction of 95.06%.

Conclusions

The results of the current study provided new insights into the new ruminal microbial features associated with feed efficiency in dairy cows, which may improve the ability to select animals for better performance in the dairy industry. The fundamental knowledge will also inform future interventions to improve feed efficiency in dairy cows.
Pregnancy outcomes in women with active anorexia nervosa: a systematic review
Authors: Pan Jeremy Ryan , Li Tina Yutong , Tucker Danny , Chen Kai Yang
Published: February 2022

Background

It is a common misconception that women with active anorexia nervosa (AN) are less likely to conceive. Pregnancies in women with AN are considered high risk. The purpose of this systematic review was to explore pregnancy complications in women with active AN, including maternal, fetal, and neonatal complications.

Methods

The authors conducted a systematic review in accordance with PRISMA statement guidelines with stringent selection criteria to include studies on patients with active AN during pregnancy.

Results

There were 21 studies included in our review. Anaemia, caesarean section, concurrent recreational substance use, intrauterine growth restriction, preterm birth, small-for-gestation (SGA) birth, and low birth weight were the most reported pregnancy complications in women with active AN, while the rates of gestational diabetes and postpartum haemorrhage were lower.

Discussion

Women with active AN have a different profile of pregnancy complications comparing to malnourished women and women in starvation. We recommend early discussion with women diagnosed with AN regarding their fertility and pregnancy complications. We recommend clinicians to aim to improve physical and psychological symptoms of AN as well as correction of any nutritional deficiency ideally prior to conception. Management of pregnancies in women with active AN requires regular monitoring, active involvement of obstetricians and psychiatrist. Paediatric follow-up postpartum is recommended to ensure adequate feeding, wellbeing and general health of the infants. Psychiatric follow-up is recommended for mothers due to risk of worsening symptoms of AN during perinatal period.