bluuebunny/crossref_metadata_2025 huggingface dataset:
Sistem Deteksi Kebocoran Gas Sederhana Berbasis Arduino Uno
Authors: Hidayat Nurul , Hidayat Samsul , Pramono Nugroho Adi , Nadirah Ulfa
Published: July 2020

Penggunaan gas di berbagai sendi kehidupan manusia sekarang ini tidak dapat terhindarkan, mulai dari kebutuhan skala rumah tangga hingga industri. Dalam upaya meningkatkan pencegahan terjadinya kecelakaan kerja akibat kebocoran gas, kontrol terhadap kebocoran gas merupakan sebuah tindakan yang vital. Oleh karena itu, makalah ini membahas respon sensor gas MQ2, MQ3, dan MQ5 terhadap gas dan asap. Ketiga sensor gas tersebut dijalankan oleh mikrokontroler ATMega328 dengan modul Arduino Uno. Sebagai aktuator, buzzer digunakan sebagai pengirim sinyal audio dan LED sebagai basis informasi visual terkait pengingat adanya gas berlebih yang terdeteksi oleh MQ2, MQ3, dan MQ5. Secara mekanisme fisis, ketika sensor gas mendeteksi adanya gas berlebih, maka sinyal suara dari buzzer akan aktif sedemikian rupa sehingga dapat digunakan sebagai indikasi dini terhadap keberadaan atau kebocoran gas. Secara umum, sensor gas MQ2, MQ3, dan MQ5 memiliki respon yang sedikit berbeda pada jenis asap dan gas yang berbeda. Pada gilirannya nanti, hasil dari penelitian ini dirancang sebagai informasi penting dalam pemilihan jenis sensor gas sesuai kebutuhan.  Simple Gas Leakage Detection System Based on Arduino UnoAbstractThe use of gas in various aspects of human life nowadays is inevitable, ranging from the needs of household scales to industries. As an effort to increase the prevention of occupational accidents due to gas leakage, control of gas leakage becomes a vital action. Therefore, this paper discusses the response of MQ2, MQ3, and MQ5 gas sensors to gases and smokes. The ATMega328 microcontroller ran the three gas sensors with the Arduino Uno module. As an actuator, buzzers were used as audio signal senders and LEDs as a basis for visual information related to reminders of excess gas detected by MQ2, MQ3, and MQ5. The physical mechanism, when the gas sensor detects the presence of excess gas, then the sound signal from the buzzer could be activated in such a way that it can be used as an early indication of the presence of leakage of gas. Generally, MQ2, MQ3, and MQ5 gas sensors performed slightly differently in responding to the presence of gases and smokes. In turn, the results of this study are designed as relevant information in selecting the type of gas sensor as needed.Keywords: Gas sensor, Microcontroller, MQ2, MQ3, MQ5, Simple instrumentation.
Pemodelan Keruntuhan Bendungan Menggunakan HEC-RAS 2D Studi Kasus Bendungan Gondang, Kabupaten Karanganyar
Authors: Murdiani Kiki Marina , Sangkawati Sri , Sadono Kresno Wikan
Published: July 2020

Pembangunan bendungan memberikan manfaat untuk masyarakat sekitar waduk, namun juga menyimpan bahaya jika mengalami keruntuhan bendungan. Salah satu syarat yang harus dipenuhi sebelum mendapatkan sertifikat ijin operasi adalah RTD (Rencana Tindak Darurat). Manfaat dari disusunnya rencana tindak darurat adalah untuk pedoman para pengelola bendungan maupun pemerintah yang berada pada daerah yang terkena resiko apabila terjadi kondisi darurat pada Bendungan. Makalah ini mencakup daerah yang terdampak jika terjadi kegagalan bendungan. Pemanfaatan Software menggunakan HEC-RAS dengan studi kasus Bendungan Gondang yang berlokasi di Kabupaten Karanganyar. Lokasi terdampak menurut hasil analisis adalah Desa Gempolan, Desa Ganten, Desa Kwadungan, Desa Kutho, Kecamatan Kerjo, Kabupaten Karanganyar, Provinsi Jawa Tengah. Bendungan ini mempunyai tampungan total 9,15 juta meter kubik dengan tinggi bendungan 71 meter.  Pemodelan keruntuhan bendungan dengan 2D pada kondisi unsteady flow, simulasi keruntuhan akibat piping tengah pada elevasi +495 m. Hasil pemodelan keruntuhan Bendungan Gondang yang akan menimbulkan dampak paling besar jika terjadi piping tengah akibat banjir PMF dengan puncak debit Qoutflow= 902,40m3/det. Kecepatan banjir akibat keruntuhan bendungan tercepat yang sampai ke pemukiman adalah 24 menit yaitu sampai di Desa Gempolan yang berjarak 0,6 km dari lokasi bendungan.AbstractThe construction of a dam provides benefits to the community around the reservoir, but also saves danger if it experiences dam collapse. One of the requirements that must be met before obtaining an operating permit certificate is the RTD (Emergency Action Plan). The benefit of formulating an emergency action plan is to guide the dam managers and the government who are in the area at risk if an emergency condition occurs at the Dam. This paper covers the area affected in the event of dam failure. Utilization of Software uses HEC-RAS with a Gondang Dam case study located in Karanganyar Regency. The affected locations, according to the results of the analysis are Gempolan Village, Ganten Village, Kwadungan Village, Kutho Village, Kerjo District, Karanganyar Regency, Central Java Province. This Dam has a total reservoir of 9.15 million cubic meters with a dam height of 71 meters. Dam failure modeling with 2D under unsteady flow conditions, simulation of collapse due to middle piping at +495 m elevation. The results of the Gondang Dam collapse modeling, which will have the most significant impact if there is middle piping due to PMF flooding with peak discharge Qoutflow = 902.40m3 / sec. The speed of flooding due to the collapse of the Dam that reached the fastest settlement was 24 minutes, namely to the village of Gempolan, which is 0.6 km from the location of the Dam.Keywords: Gondang Dam, dam break, affected area Karanganyar.
ETHICAL AND LEGAL ASPECTS OF THE USE OF FAKE DRUGS FOR OROFACIAL AESTHETICS – A CASE REPORT
Authors: Ibrahim Evelyn , Franco Ademir , Fernandes Mário Marques , Miamoto Paulo
Published: July 2020

Introduction: Over the last decade, Botulinum Toxin (BT) gained space in the armamentarium of drugs used for orofacial aesthetics. The contemporary scenario of Dentistry led to new horizons within aesthetic treatments founded on the application of BT. Objective: This study aimed to report a case of fake type-A BT sold to a dentist on training for orofacial aesthetics. Material and methods: During a 16-hour training course, a dentist bought form a reference seller a “so-called” quality drug allegedly consisting of type-A BT. After suspecting of fake drug based on uncommon label and flask, the dentist sent images of the product to the laboratory responsible for registering the drug at the National Health Surveillance Agency (ANVISA). Results: The laboratory confirmed the fake drug. ANVISA blocked the commercialization, use and distribution of the product as well as made efforts to remove the available products from the market. Parallel police investigations were carried out and forensic expertise for drug profiling was accomplished – confirming the fake drug. Conclusion: This study drives attention to the possibility of existing fake drug commercialization to dentists dedicated to orofacial aesthetics, and the potential impact of this drugs if used in patients. Consequences might extend to penalties in the consumer relation interface, as well as in the criminal field as offense against public health.
Social marketing interventions to promote physical activity among 60 years and older: a systematic review of the literature
Authors: Goethals Luc , Barth Nathalie , Hupin David , Mulvey Michael S. , Roche Frederic , Gallopel-Morvan Karine , Bongue Bienvenu
Published: July 2020

Background: Falls are a significant source of morbidity in people aged 65 and over, affecting one in three people in this age group. The scientific evidence indicates that physical activity is the most effective method for preventing falls among seniors. Although public health professionals often use social marketing to design and plan successful interventions, its use to promote physical activity and prevent falls among older people remains low. This article aims to provide a new systematic literature review of social marketing interventions promoting physical activity and targeting people aged 60 and over.Methods: Following CRD’s guidance and PRISMA guidelines, we searched between January 2008 and July 2019 for relevant articles in five primary databases using predefined search and inclusion criteria. Two independent reviewers analysed the selected articles to identify evidence of the seven social marketing benchmark criteria, defined by experts in the field as the common elements that contribute to social marketing success.Results: The final review included nine studies. Of the studies selected, three specifically targeted over 60-year-olds, whereas the others segmented the population into several age-based subcategories, including over 60-year-olds. Eight studies highlighted positive results for the participants with an increase in participation or an increase in physical activity level. None of the nine studies selected for this systematic review implemented the entire social marketing approach.Conclusion: Few published interventions use the seven social marketing criteria. Further research is required to encourage uptake and inclusion in successful social marketing interventions to increase program effectiveness in this target population.
Inequity under Equality: Research on the Benefits Equity of Chinese Basic Medical Insurance
Authors: Diao Li , Liu Yiwei
Published: July 2020

Background: The pursuit of equity is one of the basic principles behind the strengthening of health care reform. China's new rural cooperative medical insurance (NRCMI) and urban residents' basic medical insurance (URBMI) are both “equalized” in terms of fundraising and reimbursement. This paper studies the benefits equity under this "equalized" system.Methods: The data analysed in this paper are from the China Family Panel Studies (CFPS) from 2014 to 2016, implemented by the Institute of Social Science Survey at Peking University. A two-part model and a binary choice model are used in the empirical test.Results: The empirical test revealed that high-income people benefit more from basic medical insurance than low-income people. Mechanism analysis demonstrated that high-income people have higher medical insurance applicability and can utilize better health care. Since low-income people are unhealthier, inequity in benefits exacerbates health inequity. We also found that the benefits equity of URBMI is better than that of NRCMI.Conclusions: The government needs to pay more attention to the issue of medical insurance inequity. We should consider allowing different income groups to pay different premiums according to their medical expenses or applying different reimbursement policies for different income groups.
Barriers and motivators to participation and retention in HIV/HCV cohort studies among people who inject drugs: A community consultation in Iran
Authors: mirzazadeh ali , Hooshyar Samira Hosseini , Shahesmaeili Armita , Bahramnejad Ali , Barsham Adibeh , Moosavian Ghazal , Najafi Ismail , Sharifi Hamid , Haghdoost Ali-Akbar , Briceno Alya , McFarland Willi , Page Kimberly
Published: July 2020

Background: The lack of robust estimates of HIV/HCV incidence among people who inject drugs (PWID) in Iran calls for well-designed prospective cohort studies. Successful recruitment and follow-up of PWID in cohort studies may require formative assessment of barriers PWID are faced with in participation and retention in cohort studies and factors they think may facilitate their engagement in such studies. Using a focus group discussion (FGD) format, we conducted a consultation with PWID in southeast Iran to recognize those barriers and motivators.Methods: Using targeted sampling and through snowball referrals, we recruited PWID (aged≥18, injected in last 6 months) from community-based drop-in centers (DICs), homeless shelters, and through outreach efforts to participate in four FGDs (one women-only). Socio-demographic characteristics, injection behaviors and self-reported HCV/HIV testing and diagnosis history were obtained. Then, a semi-structured FGD guide was applied to explore barriers and motivators to participation and retention in cohort studies among study participants. All FGD sessions were recorded and transcribed verbatim, removing any identifying information. The content of FGDs were analyzed by thematic analysis using an inductive approach.Results: In total, 30 individuals (10 women) participated in the study. The median age of participants was 35 (IQR 31-40), with majority (73.3%) reporting injecting drug use within the last month. Only 40.0% reported ever being tested for HCV whereas a larger proportion (63.4%) reported ever being tested for HIV. While the majority were willing to participate in cohort studies, breach of confidentiality, fear of positive test results, perceived required commitment, and marginalization were reported as barriers to participation and retention in such studies. Monetary incentive, the thought of a better life, protection from police interventions and trust between health workers and PWID were addressed as motivators of engagement in cohort studies among PWID.Conclusions: Strategies to enhance data security and reduce stigma associated with injecting drug use along with involving peer workers in research, providing pre and post-test counselling and education and addressing the needs of more marginalized groups potentially through integrated healthcare programs and housing support are among few approaches that may help address barriers and strengthen the motivators for successful cohort studies among this population.
Evaluating a multidimensional strategy to improve the professional self-care of occupational therapists working with people with life limiting illness.
Authors: Apostol Courtney , Cranwell Kathryn , Hitch Danielle
Published: July 2020

Background: The term ‘’life limiting conditions’ refers to premature death following decline from chronic conditions, which is a common circumstance in which occupational therapists work with people at the end of life. The challenges for clinicians of working with these patients have long been recognised, and may have a significant impact on their professional self-care. This study aimed to evaluate a multidimensional workplace strategy to improve the professional self-care of occupational therapists working with people living with a life limiting condition. Methods: A pre and post mixed methods survey approach were utilised, with baseline data collection prior to the implementation of a multidimensional workplace strategy. The strategy included professional resilience education, targeted supervision prompts, changes to departmental culture and the promotion of self-care services across multiple organisational levels. Follow up data collection was undertaken after the strategy had been in place for two years. Quantitative data were analysed descriptively, while qualitative data were subjected to thematic analysis. Results: 103 occupational therapists responded (n=55 pre, n=48 post) across multiple service settings. Complex emotional responses and lived experiences were identified by participants working with patients with life limiting conditions, which were not influenced by the workplace strategy. Working with these patients was acknowledged to challenge the traditional focus of occupational therapy on rehabilitation and recovery. Participants were confident about their ability to access self-care support, and supervision emerged as a key medium. While the strategy increased the proportion of occupational therapists undertaking targeted training, around half identified ongoing unmet need around professional self-care with this patient group. Demographic factors (e.g. practice setting, years of experience) also had a significant impact on the experience and needs of participants. Conclusions: The multidimensional workplace strategy resulted in some improvements in professional self-care for occupational therapists, particularly around their use of supervision and awareness of available support resources. However, it did not impact upon their lived experience of working with people with life limiting conditions, and there remain significant gaps in our knowledge of support strategies for self-care of occupational therapist working with this patient group.
Characterization of total adenosine deaminase activity (ADA) and its isoenzymes in saliva and serum in health and inflammatory conditions to four different species: an analytical and clinical validation pilot study
Authors: Contreras-Aguilar María Dolores , Tvarijonaviciute Asta , Monkeviciene Ingrida , Martín-Cuervo María , Gonzales Luis Guillermo , Franco Lorena , Cerón José Joaquín , Tecles Fernando , Escribano Damian
Published: July 2020

Background: Measurement of adenosine deaminase (ADA) can provide information about cell-mediated immunity. This report’s objetive was to study the enzymatic activity of total ADA (tADA) and its isoenzymes ADA1 and ADA2 in canine, equine, porcine, and bovine serum and saliva and their changes in different inflammatory situations in each species. Besides, an automated method for ADA2 measurement was developed and validated.Results: tADA was present in serum and saliva of healthy animals of the four species. Erythro-9-(2-hydroxy-3-nonyl) adenine (EHNA) concentration of 0.47mM was needed for ADA1 inhibition in canine and porcine samples (serum and saliva) and bovine saliva, whereas for equine saliva 0.94mM was needed. ADA2 activity was not detected in bovine serum and was very low or absent in equine serum and bovine saliva. An automated procedure to measure ADA2 consisting of adding EHNA to a commercial reagent for tADA measurement provided repetitive (coefficients of variation <8.8% in serum and <10% in saliva) and accurate (linearity under dilution approaches gave R 2 >0.90) results, being equivalent to a manual incubation of the sample with EHNA at a similar concentration. Salivary tADA, as well as ADA1 and ADA2, were higher in dogs with leishmaniosis, horses with acute abdominal disease and pigs with lameness than in healthy animals. ADA showed significant correlation with serum ferritin in dogs ( r = 0.602, P <0.01; r = 0.555, P <0.05; and r = 0.632, P <0.01; respectively for tADA, ADA1 and ADA2) and serum C-reactive protein in pigs ( r = 0.700, P <0.01, for both tADA and ADA1; r = 0.770, P <0.001, for ADA2), whereas salivary ADA2 significantly correlated with serum amyloid A in the horse ( r = 0.649, P <0.01). In the cow, salivary tADA and ADA1 significantly increased after calving, correlatingd with total white blood cell count ( r = 0.487, P <0.05, both).Conclusions: ADA activity, as well as its isoenzymes, can be measured in serum and saliva of different animal species. The salivary levels of these analytes could potentially be inflammatory biomarkers in these species.
Integrated sRNAome and RNA-Seq Analysis Reveals miRNA Effects on Betalain Biosynthesis in Pitaya
Authors: Chen Canbin , Xie Fangfang , Hua Qingzhu , Zur Noemi Tel , Zhang Lulu , Zhang Zhike , Zhang Rong , Zhao Jietang , Hu Guibing , Qin Yonghua
Published: July 2020

Background: MicroRNAs (miRNAs) and their regulatory functions in anthocyanin, carotenoid, and chlorophyll accumulation have been extensively characterized in many plant species. However, the miRNA regulatory mechanism in betalain biosynthesis remains mostly unknown. Results: In this study, 126 conserved miRNAs and 41 novel miRNAs were first isolated from Hylocereus monacanthus, among which 95 conserved miRNAs belonged to 53 miRNA families. 34 candidate miRNAs related to betalain biosynthesis were differentially expressed. The expression patterns of those differential expressed miRNAs were analyzed in various pitaya tissues by RT-qPCR. A significantly negative correlation was detected between the expression levels of half those miRNAs and corresponding target genes. Target genes of miRNAs i.e. Hmo-miR157b-HmSPL6-like, Hmo-miR160a-Hpcyt P450-like3, Hmo-miR6020-HmCYP71A8-like, Hmo-novel-2-HmCYP83B1-like, Hmo-novel-15-HmTPST-like, Hmo-miR828a-HmTT2-like, Hmo-miR858-HmMYB12-like, Hmo-miR858-HmMYBC1-like and Hmo-miR858-HmMYB2-like were verified by 5′RACE and transient expression system in tobacco.Conclusions: Hmo-miR157b, Hmo-miR160a, Hmo-miR6020 Hmo-novel-2, Hmo-novel-15, Hmo-miR828a and Hmo-miR858 play important roles in pitaya fruit coloration and betalain accumulation. Our findings provide new insights into the roles of miRNAs and their target genes of regulatory functions involved in betalain biosynthesis of pitaya.
Interrelation Between Defensive Mechanisms and Coping Strategies in Psychiatry Trainees in Romania: A Multicenter Study
Authors: Panfil Anca-Livia , Frandes Mirela , Nirestean Aurel , Hurmuz Marinela , Lungeanu Diana , Cristanovici Madalina , Lemeti Laura , Isac Andra , Papava Ion , Bredicean Cristina
Published: July 2020

Background: The challenges faced by professionals when working in the field of psychiatry require the development of adequate defensive and coping mechanisms. This study aimed to explore both coping strategies and defense mechanisms and their relationship in psychiatry trainees in Romania.Methods: A cross-sectional study was conducted to determine and evaluate both defensive and coping mechanisms of Romanian psychiatry trainees. Defensive Style Questionnaire-60 and COPE scale were applied to psychiatry trainees from five training centers in Romania. By applying structural equation modeling, models that presumed the existence of relationships between coping strategies and defensive mechanisms were analyzed. Results: Superior defense mechanisms and task-oriented coping strategies were the commonly used approaches by psychiatry trainees. Furthermore, significantly consistent correlations (ranging from 0.2 to 0.5) between adaptive defense mechanisms and coping strategies focused on the problem or emotion were shown. Similarly, avoidant coping strategies correlated with non-adaptive defense mechanisms (correlations between 0.3 and 0.5). Our model presented good fit indices (X2(34) = 64.324, p < 0.001; GFI = 0.93; root mean square error = 0.08). Moreover, the results indicated a weak association between the two types of adaptive processes (r = 0.07, p < 0.001).Conclusion: Psychiatry trainees present a profile based on two independent groups of adaptation processes, namely, adaptive defenses and problem-oriented coping scales and non-adaptive defenses and avoidant coping scales.
Prediction of different airway gradings in T1-2 laryngeal cancer with anterior vocal commissure involvement
Authors: Luo Chunlin , Zheng Ying , Lei Wenbin
Published: July 2020

Background: A retrospective analysis was conducted to investigate the clinical efficacy of the modified Mallampati test (MMT) combined with indirect laryngoscopy in measuring glottic exposure levels of early glottic laryngeal carcinoma (T1-2) with anterior vocal commissure involvement.Methods: One hundred eighty-two patients with early glottic laryngeal cancer were divided into two groups: one group comprised patients treated with transoral carbon dioxide laser microsurgery (TLM, 65 patients), and the other group comprised patients treated with open partial laryngectomy (OPL, 117 patients). The MMT and indirect laryngoscopy were used to re-evaluate the level of glottic exposure and to classify the patients based on these levels. The local control and disease-free survival rates of those patients were measured based on their MMT and indirect laryngoscopy classifications.Results: The 5-year local control rate was significantly different between the two groups according to the MMT (log-rank test: χ2=4.020, P=0.045, 90.9% in the OPL group vs. 71.4% in the TLM group). For patients diagnosed with grade IV tumors using indirect laryngoscopy, the 5-year local control rate was significantly different between the two groups (log-rank test: χ2=4.076, P=0.044, 91.7% in the OPL group vs. 54.7% in the TLM group). Among patients diagnosed with grade III or IV tumors using indirect laryngoscopy and with class III or IV tumors using MMT, the 5-year local control rate was significantly different between the two groups (log-rank test: χ 2 = 8.037, P = 0.005, 97.14% in the OPL group vs. 75.47% in the TLM groups).Conclusions: The combination of the MMT and indirect laryngoscopy to evaluate difficult airways is relatively simple and is very useful for surgeons to better prepare for surgery.Trial registration: ChiCTR-ORC-17010515
Protocol registration issues of systematic review and meta-analysis studies: a survey of global researchers
Authors: Tawfik Gehad Mohamed , Giang Hoang Thi Nam , Ghozy Sherief , Altibi Ahmed M. , Kandil Hend , Le Huu-Hoai , Eid Peter Samuel , Radwan Ibrahim , Makram Omar Mohamed , Hien Tong Thi Thu , Sherif Mahmoud , Hossain As-Saba , Thang Tai Luu Lam , Puljak Livia , Salem Hosni , Numair Tarek , Moji Kazuhiko , Huy Nguyen Tien
Published: July 2020

Background: Although protocol registration of systematic reviews/meta-analysis (SR/MA) is still not mandatory, it is highly recommended that authors publish their SR/MA protocols prior to submitting their manuscripts for publication as recommended by the Cochrane guidelines for conducting SR/MAs. our aim was to assess the awareness, obstacles, and opinions of SR/MA authors about the protocol registration process. Methods: A cross-sectional survey study included the authors who published SR/MAs during the period from 2010 to 2016, and they were contacted for participation in our survey study. They were identified through the literature search of SR/MAs in Scopus database. An online questionnaire was sent to each participant via e-mail after receiving their approval to join the study. We have sent 6650 emails and received 275 responses.Results: A total of 270 authors responses were complete and included in the final analysis. Our results has shown that PROSPERO was the most common database used for protocol registration (71.3%). The registration-to-acceptance time interval in PROSPERO was less than one month (99.1%). Almost half of the authors (44.2%) did not register their protocols prior to publishing their SR/MAs and according to their opinion that the other authors lack knowledge of protocol importance and mandance to be registered, was the most commonly reported reason (44.9%). A significant percenatge of respondents (37.4%) believed that people would steal their ideas from protocol databases, while only 5.3% reported that their SR/MA had been stolen. However, the majority (72.9%) of participants have agreed that protocol registries play a role in preventing unnecessary duplication of reviews. Finally, 37.4% of participants agree that SR/MA protocol registration should be mandatory. Conclusion: About half of the participants believes that the main reason for not registering protocols, is that the other authors lack knowledge concerning obligation and importance to register the SR/MA protocols in advance. Therefore, tools should be available to mandate protocol registration of any SRs beforehand and increasing awareness about the benefits of protocol registration among researchers.
“It was like nobody cared about what I said?” Iranian women committed self- immolation: A qualitative study
Authors: Aghakhani Nader , Lopez Violeta , Parizad Naser , Baghaei Rahim
Published: July 2020

Background: Suicide-attempts have increased across the world and have become more higher among females. In Iran, there has been a high prevalence of self-immolation mostly young married women admitted in the burn centers. This study aimed to explore the factors and experiences of self-immolation in Iranian married women to develop prevention strategies to prevent the personal, social and economic impacts of suicide and suicide attempts. Methods: A qualitative descriptive approach using open-ended in-depth face to face interviews was conducted in a purposive sample of 16 married Iranian women aged 16 to 40 years in the burn centers in Urmia city located in northwest Iran. Conventional content analysis was used to analyze the data.Results: Four themes emerged from the data including: (1) antecedents of self-immolation, (2) suicidal ideation method, (3) defeat and humiliation, and (4) pathway to recovery. Each of these themes are supported by sub-themes.Conclusions: The study highlights the need for health professionals to focus in helping and supporting self-immolation survivors to continue a normal life. A comprehensive supportive program based on survivors’ needs to support their pathways to recovery in all its complexities is recommended. Health professional should also not forget that the family of the survivors also will need help to overcome this trauma. Family counselling program may also be provided.
Functional hyperemia drives fluid exchange in the paravascular space
Authors: Kedarasetti Ravi , Turner Kevin L. , Echagarruga Christina , Gluckman Bruce J. , Drew Patrick J. , Costanzo Francesco
Published: July 2020

The brain lacks a conventional lymphatic system to remove metabolic waste. It has been proposed that directional fluid movement through the arteriolar paravascular space (PVS) promotes metabolite clearance. We performed simulations to examine if arteriolar pulsations and dilations can drive directional CSF flow in the PVS and found that arteriolar wall movements do not drive directional CSF flow. We propose an alternative method of metabolite clearance from the PVS, namely fluid exchange between the PVS and the subarachnoid space (SAS). In simulations with compliant brain tissue, arteriolar pulsations did not drive appreciable fluid exchange between the PVS and the SAS. However, when the arteriole dilated, as seen during functional hyperemia, there was a marked exchange of fluid. Simulations suggest that functional hyperemia may serve to increase metabolite clearance from the PVS. We measured blood vessels and brain tissue displacement simultaneously in awake, head-fixed mice using two-photon microscopy. These measurements showed that brain deforms in response to pressure changes in PVS, consistent with our simulations. Our results show that the deformability of the brain tissue needs to be accounted for when studying fluid flow and metabolite transport.Acknowledgements: This work was supported by NSF Grant CBET 1705854.
Staging Procedures Fail to Benefit Women with Borderline Ovarian Tumours Who Want to Preserve Fertility: A Retrospective Analysis of 448 cases
Authors: Li Na , Gou Jinhai , Li Lin , Ming Xiu , Hu Tingwenyi , Li Zhengyu
Published: July 2020

Background

This study aimed to evaluate the effect of clinicopathologic and surgical factors on the prognosis and fertility outcomes of patients with borderline ovarian tumour (BOT).

Methods

We performed a retrospective analysis of BOT patients who underwent surgical procedures in West China Second University Hospital from January 2008 to January 2015. The disease-free survival (DFS) outcomes and potential prognostic factors were evaluated using the Kaplan-Meier method and Cox regression analysis, respectively. Furthermore, fertility outcomes were analysed using Pearson Χ 2 and Fisher’s correlation tests.

Results

A total of 448 patients were included, with a median age of 37.1 years and a median follow-up time of 113 months; 52 (11.6%) recurrences were observed, with a mean recurrence interval of 80.2 months and four (0.9%) deaths; 118 (26.3%) patients underwent staging surgery and the remaining 330 (73.7%) underwent unstaged surgery. In total, 233 patients undergoing fertility-sparing surgery (FSS) attempted to conceive, and 92 (39.48%) of them achieved pregnancy. No significant differences in fertility outcomes were found between the staging and unstaged surgery groups ( P = 0.691). In univariate analysis, staging surgery was associated with DFS (hazard ratio [HR] = 2.191; P = 0.005), but it was not an independent prognostic factor ( P = 0.600) for DFS on multivariate analysis. Multivariate Cox analysis revealed that advanced FIGO stage (≥stage II), positive ascites\pelvic washings, and laparotomy approach were independent prognostic factors for DFS in patients with BOT, whereas advanced stage (≥stage II), laparotomy approach, cystectomy-related procedures, invasive implants, and bilateral tumours were independent prognostic factors for DFS in patients undergoing FSS. In addition, laparoscopy resulted in better prognosis than laparotomy in patients with early-stage (stage I) tumours and a desire for fertility preservation.

Conclusion

Patients with BOT fail to benefit from surgical staging in terms of prognosis and fertility outcomes. Laparoscopy is recommended for patients with stage I disease who desire to preserve their fertility. Physicians should pay more attention to the risk of recurrence in patients who want to preserve fertility with advanced stage (≥stage II) disease, invasive implants, and bilateral tumours, and choose FSS carefully.
Influence of Participation in a Quality Improvement Collaborative on Staff Perceptions of Organizational Sustainability
Authors: II James H Ford , Gilson Aaron
Published: July 2020

Background: Sustainability capacity (SC), which is an organization’s ability to implement and maintain change, is influenced by internal attributes, environmental contextual influencers, and intervention attributes. Temporal changes in staff SC perceptions, as well as the influence of quality improvement collaborative (QIC) participation, has generally not been explored. This project addresses this gap, measuring staff SC perceptions at four time points (baseline and every 9 months) for clinics participating in an intervention – the Network for the Improvement of Addiction Treatment QIC initiative (called NIATx200).Methods: A mixed linear model repeated measures analysis was applied to matched staff members (n=908, representing 2,329 total cases) across the evaluation timeframe. Three separate statistical models assessed potential predictors of SC perceptions: Time (Models I-III); NIATx200 intervention, staff job function, and tenure (Models II &III); and NIATx200 participation hours and four organizational variables (Model III).Results: For Model I, staff perceptions of total SC increased throughout most of the study (t1,4=-6.74, p<.0001; t2,4=-3.100, p<.036; t3,4=-0.23, p=ns). Model II did not change Model I’s overall Time effect, but combined NIATx200 services (t=-2.23, p=.026), staff job function (t=-3.27, p=.001), and organizational administrators (t=-3.50, p=.001) were also significantly associated with greater perceptions of total SC. Inclusion of additional variables in Model III demonstrated the importance of a higher participation level (t=-3.09, p<.002) and being in a free-standing clinic (t=-2.06, p<.04) on staff perceptions of total SC. Conclusion: Although staff exposure to sustainability principals was minimal in NIATx200, staff perceptions about their organization’s SC significantly differed over time. However, an organization’s participation level in a QIC became the principal predictor of staff SC perceptions, regardless of other factors’ influence. Given these findings, it is possible to develop and introduce specific sustainability content within the structure of a QIC to assess the impact on staff SC perceptions over time and the sustainment of organizational change.Trial Registration: ClinicalTrials.gov, NCT00934141 Registered July 6, 2009. Retrospectively registered. https://clinicaltrials.gov/ct2/show/NCT00934141
Novel mutation in the TGFBI gene in a Moroccan family with atypical corneal dystrophy
Authors: BENBOUCHTA Yahya , JAOUAD Imane CHERKAOUI , TAZI Habiba , ELORCH Hamza , OUHENACH Mouna , ZRHIDRI Abdelali , SADKI Khalid , SEFIANI Abdelaziz , LYAHYAI Jaber , BERRAHO Amina
Published: July 2020

Background: Corneal dystrophies (CDs) are a heterogeneous group of bilateral, genetically determined, non-inflammatory bilateral corneal diseases which are usually limited to the cornea. CD is characterized by a large variability in the age of onset, evolution and visual impact and the accumulation of insoluble deposits at different depths of the cornea. Clinical symptoms revealed bilaterally multiple superficial, epithelial, and stromal anterior granular opacities, in different stages of severity among three patients of this family. 99 genes are involved in CDs.The aim of this study is to identify pathogenic variant caused atypical corneal dystrophy in a large Moroccan family and to describe the clinical phenotype with their severe different stages of evolution.Methods: In this study, we report a large Moroccan family with CD. Whole Exome Sequencing (WES) was performed in the three affected members who shared a phenotype of a corneal dystrophy in different stages of severity. De variant validation and familial segregation were done by Sanger sequencing in affected sister and mothers and in two unaffected brothers.Results: Whole exome sequencing showed a novel heterozygous mutation (c.1772C>A; p.Ser591Tyr) in TGFBI gene. Clinical examinations demonstrated bilaterally multiple superficial, epithelial and stromal anterior granular opacities; in different stages of severity among three patients of this family. Conclusions: This report presents a novel mutation in TGFBI gene, found in three family members affected with different phenotypic aspects. This mutation is associated with Thiel-Behnke corneal dystrophy and therefore, it could be considered as a novel phenotype genotype correlation, which will help in genetic counseling for this family
Development of an in vitro 3D model for investigating ligamentum flavum hypertrophy
Authors: Lin Cheng-Li , Kuo Yi-Ting , Tsao Che-Hao , Shyong Yan-Jye , Shih Shu-Hsien , Tu Ting-Yuan
Published: July 2020

Background Ligamentum flavum hypertrophy (LFH) is among the most crucial factors in degenerative lumbar spinal stenosis, which can cause back pain, lower extremity pain, cauda equina syndrome and neurogenic claudication. The exact pathogenesis of LFH remains elusive despite extensive research. Most in vitro studies investigating LFH have been carried out using conventional 2D two-dimensional (2D) cell cultures, which do not resemble in vivo conditions, as they lack crucial pathophysiological factors found in three-dimensional (3D) LFH tissue, such as enhanced cell proliferation and cell cluster formation. In this study, we generated ligamentum flavum (LF) clusters using spheroid cultures derived from primary LFH tissue. Results The cultured LF spheroids exhibited good viability and growth on an ultra-low attachment 96-well plate (ULA 96-plate) platform according to live/dead staining. Our results showed that the 100-cell culture continued to grow in size, while the 1000-cell culture maintained its size, and the 5000-cell culture exhibited a decreasing trend in size as the culture time increased; long-term culture was validated for at least 28 days. The LF spheroids also maintained the extracellular matrix (ECM) phenotype, i.e., fibronectin, elastin, and collagen I and III. The 2D culture and 3D culture were further compared by cell cycle and Western blot analyses. Finally, we utilized hematoxylin and eosin (H&E) staining to demonstrate that the 3D spheroids resembled part of the cell arrangement in LF hypertrophic tissue. Conclusions The developed LF spheroid model has great potential, as it provides a stable culture platform in a 3D model that can further improve our understanding of the pathogenesis of LFH and has applications in future studies.
A simple risk-based strategy for hepatitis C virus screening among incarcerated people in a low- to middle-income setting
Authors: Hariri Sanam , Sharafkhah Maryam , Alavi Maryam , Roshandel Gholamreza , Fazel Abdolreza , Amiriani Taghi , Motamed-Gorji Nazgol , Bazazan Abolfazl , Merat Shahin , Poustchi Hossein , Malekzadeh Reza
Published: July 2020

Background: Hepatitis C virus (HCV) is among the highest priority diseases in custodial settings; however, the diagnosis remains suboptimal among people in custody. This study aimed to validate a short survey for identifying people with HCV infection in a provincial prison in Iran.Methods: Between July and December 2018, residents and newly admitted inmates of Gorgan central prison completed a questionnaire, including data on the history of HCV testing, drug use, injecting drug use, sharing injecting equipment, and imprisonment. Participants received rapid HCV antibody testing, followed by venipuncture for RNA testing (antibody-positive only). Each enrollment question (yes/no) was compared with the testing results (positive/negative).Results: Overall, 1892 people completed the questionnaire, including 621 (34%) who were currently on opioid agonist therapy (OAT); 30% of participants had been tested for HCV previously. About 71% had a history of drug use, of whom 13% had ever injected drugs; 56% had ever shared injecting equipment. Prevalence of HCV antibody and RNA was 6.9% (n=130) and 4.8% (n=90), respectively. The antibody prevalence was higher among people on OAT compared to those with no history of OAT (11.4% vs. 4.0%). History of drug use was the most accurate predictor of having a positive HCV antibody (sensitivity: 95.2%, negative predictive value: 98.9%) and RNA testing (sensitivity: 96.7%, negative predictive value: 99.5%). Sensitivity of the drug use question was lowest among people with no OAT history and new inmates (87% and 89%, respectively). Among all participants, sensitivity and negative predictive value of the other questions were low and ranged from 34% to 54% and 94% to 97%, respectively.Conclusions: In resource-limited settings, HCV screening based on having a history of drug use could replace universal screening in prisons to reduce costs. Developing tailored screening strategies together with further cost studies are crucial to address the current HCV epidemic in low- to middle-income countries.
The prevalence of the Neglected Sexual Side Effects (NSSEs) after early prostate cancer treatment and the role of a questionnaire in its diagnosis: A Scoping Review Protocol
Authors: Roscher Pierre , Wyk Jacqueline M Van
Published: July 2020

Background: Early prostate cancer (PCa) treatment interventions may leave men with debilitating sexual side effects. These side effects may remain permanent, often undiagnosed and undermanaged. The objective of this study is to map the evidence about the prevalence, and use of questionnaires, related to the neglected sexual side effects (NSSE) after PCa treatment.Methods: This systematic scoping review's search strategy will involve the following electronic databases: PubMed, Science Direct and Google Scholar. Following title searching, two-independent reviewers will conduct screening of abstracts and full articles. Eligibility criteria will guide the screenings. Data will be extracted from the included studies, and the emerging themes will be analysed. The review team will analyse the implications of the findings concerning the research question and aim of the study. The Mixed Method Appraisal Tool (MMAT) will be employed for quality appraisal of included studies.Discussion: We anticipate finding a small number of studies about the prevalence and questionnaire use for NSSE after early PCa treatment. The study findings will be disseminated through publication in a peer-reviewed journal, peer presentations, as well as presentations at relevant conferences.
UGT1A1 polymorphism has a prognostic effect in patients with stage IB or II uterine cervical cancer and one or no metastatic pelvic nodes receiving irinotecan chemotherapy
Authors: Matsuoka Hideki , Murakami Ryusuke , Abiko Kaoru , Yamaguchi Ken , Horie Akihito , Hamanishi Junzo , Baba Tsukasa , Mandai Masaki
Published: July 2020

Background : Uridine diphosphate glucuronosyltransferase 1 family polypeptide A1 (UGT1A1 ) is a predictive biomarker for the side-effects of irinotecan; irinotecan chemotherapy reduces the volume of tumors harboring UGT1A1 polymorphisms. We aimed to determine whether UGT1A1 polymorphisms can predict progression-free survival in patients with local cervical cancer treated with irinotecan. Methods : We retrospectively analyzed the data of 51 patients with cervical cancer treated at a single institution between 2010 and 2015. All patients were diagnosed with the 2009 International Federation of Gynecology and Obstetrics (FIGO) stage IB1, IB2, IIA, or IIB squamous cell carcinoma, underwent radical hysterectomy, and received irinotecan chemotherapy as neoadjuvant and/or adjuvant treatment. All patients were examined for irinotecan side effects using UGT1A1 tests. Conditional inference tree and survival analyses were performed considering stage, age, UGT1A1 status, and the number of metastatic lymph nodes to determine primary factors associated with progression-free survival. Results : The tree-structured survival model determined high recurrence-risk factors related to progression-free survival. The most relevant factor was ≥2 metastatic lymph nodes (p = 0.004). The second most relevant was UGT1A1 genotype (p = 0.024). Among patients with ≤1 metastatic lymph node, those with UGT1A1 polymorphisms benefited from irinotecan chemotherapy and demonstrated significantly longer progression-free survival (p = 0.020) than those with wild-type UGT1A1 . Conclusion : Irinotecan chemotherapy has the potential to benefit patients with cervical cancer, UGT1A1 polymorphism, and ≤1 metastatic lymph nodes.
Under-five children’s Bacterial Blood Stream Infection in Southern Ethiopia
Authors: zenebe mengistu hailemariam , Alemayehu Tsegaye A , Agegnehu Asnakech A , Ali Mulubrhan A , Mitiku Enkosilassie M , Azerefegne Elshaday A
Published: July 2020

Background

Under-five children’sblood stream infectionsmainly due to resistant pathogens found to be a higher risk of hospital mortality in low and middle income countries. This study intended to assess the bacterial profile and drug resistance of isolates of blood stream infection among under- 5 children in Southern Ethiopia.
Methods

Retrospectively lab recordeddata’s were retrieved to assess the bacterial isolates and rates antibiotic resistance at Hawassa University Comprehensive Specialized Hospital.
Results

Of 323bloods culture performed116 (35.9%) were positive for potential bacterial BSI. Positivity of blood culture decrease with age, hence neonatesblood stream infectionstream infection werehigher than other age groups (OR, 3; 95% CI 1.5–5.1; p = 0.001). The major gram positive isolateCoNSand S.aureushave shown highest level of resistance to penicillin 61.3.0% and tetracycline 78.8% whereas less level of resistance was reported to Meropenem 6.4%, Ceftriaxone 13% and doxycycline 13%. Although most of gram negatives isolates were resistant for tested antibiotics, K.pneumoniae has shown leaser resistance tocefotaxime and chloramphenicol.
Conclusion

A high level of blood stream infection accompanied by high drug resistant isolates was reported. The great burden in neonate than other children was reported. As a result to reduce the burden the hospital administrators need tofacilities effective infection prevention and control programmes, effective hygiene practices and well-functioning environment along with educating patient about proper hygiene practices.