bluuebunny/crossref_metadata_2025 huggingface dataset:
Nutritional Quality of the Most Consumed Varieties of Raw and Cooked Rice in Spain Submitted to an In Vitro Digestion Model
Authors: Aguilera-Velázquez José Raúl , Carbonero-Aguilar Pilar , Martín-Carrasco Irene , Hinojosa María Gracia , Moreno Isabel , Bautista Juan
Published: October 2021

Rice is one of the most consumed staple foods around the world and its trade is highly globalized. Increased environmental pollution generates a large amount of waste that, in many cases, is discarded close to culture fields. Some species are able to bioaccumulate toxic substances, such as metals, that could be transferred to the food chain. The main goal of this study was to evaluate the content of metallic (Al, Cd, Pb, and Cr) and metalloid elements (As) in 14 of the most consumed varieties of rice in Spain and their effects on human health. The samples were cooked, and human digestion was simulated by using a standard in vitro digestion method. Metallic and metalloid element levels were analysed by Inductively Coupled Plasma Optical Emission Spectrometry (ICP-OES), previous called microwave digestion. Both the human health risk index, Hazard Quotient, and Lifetime Cancer Risk did not show toxic values in any case. Rice with a higher non-digestible fraction showed a higher liberation of proteins and a lower glycemic index. There were no significant differences in the concentrations of metallic and metalloid elements in cooked rice or in the digestible fraction in all varieties analysed. However, Al concentrations were higher than other metals in all varieties studied due to its global distribution. No relationship has been observed between the digestibility of rice and the bioaccessibility of each metallic and metalloid element. All of the studied rice varieties are healthy food products and its daily consumption is safe. The regular monitoring of metals and As in rice consumed in Spain may contribute to improvements in the human health risk evaluation.
Aquafaba from Korean Soybean II: Physicochemical Properties and Composition Characterized by NMR Analysis
Authors: He Yue , Shim Youn Young , Shen Jianheng , Kim Ji Hye , Cho Jae Youl , Hong Wan Soo , Meda Venkatesh , Reaney Martin J. T.
Published: October 2021

Aquafaba (AQ) emulsification properties are determined by genetics and seed processing conditions. The physicochemical properties and hydration rates of chickpea (CDC Leader) as a control with proven emulsifying properties were recently reported. Here, we identify correlations between soybean (Backtae, Seoritae, and Jwinunikong) physical, chemical, and hydration properties as well as AQ yield from seed and functional (emulsion and foaming) properties. In addition, a total of 20 compounds were identified by NMR including alcohols (isopropanol, ethanol, methanol), organic acids (lactic acid, acetic acid, succinic acid, citric acid, and malic acid), sugars (glucose, galactose, arabinose, sucrose, raffinose, stachyose), essential nutrients (choline, phosphocholine), amino acids (alanine, glutamine), and polyphenols (resveratrol, glycitin). The process used in this study utilizes a soaking step to hydrate the seed of the selected Korean soybean cultivars. The product, AQ, is an oil emulsifier and foaming agent, which is suitable for use as an egg substitute with improved emulsion/foam formation properties when compared with a chickpea-based AQ.
Editorial for Special Issue “Fractional Behavior in Nature 2019”
Authors: Ortigueira Manuel Duarte
Published: October 2021

The presence of fractional behavior in nature is unquestionable [...]
An Experiment on Cooperation in a CPR Game with a Disapproval Option
Authors: Yao Koffi Serge William
Published: October 2021

This paper studies the standard version of the approval mechanism with two players in a common pool resource (CPR) extraction game. In the case of disapproval, the Nash extraction level is implemented. The paper investigates, experimentally, the extent to which the Nash threat leads to Pareto-improving extraction levels. Through our experiment, we confirm the effectiveness of the Nash threat in reducing CPR over-extraction. Although participants’ behavior is mainly explained by rational thinking, inequity in payoff can also motivate their behavior. Moreover, we show that there is neither an order effect nor a framing effect. Finally, the reduction persists when the Nash threat is no longer in place.
Highly Crosslinked Agar/Acrylic Acid Hydrogels with Antimicrobial Properties
Authors: Pino-Ramos Victor H. , Duarte-Peña Lorena , Bucio Emilio
Published: October 2021

Hydrogels are three-dimensional soft polymeric materials that can entrap huge amounts of water. They are widely attractive in the biomedicine area because of their outstanding applications such as biosensors, drug delivery vectors, or matrices for cell scaffolds. Generally, the low mechanical strength and fragile structure of the hydrogels limit their feasibility, but this is not the case. In this work, acrylic acid–agar hydrogels with excellent mechanical properties were synthesized using gamma radiation as a crosslinking promoter. The obtained hydrogels exhibited a water absorption capacity up to 6000% in weight without breaking and keeping their shape; additionally, they showed a noticeable adhesion to the skin. The synthesized materials were characterized by infrared spectroscopy (FTIR-ATR), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), scanning electron microscopy (SEM), and mechanical testing. Additionally, their water uptake capacity and critical pH were studied. Net(Agar/AAc) hydrogel exhibited a noticeable capacity to load silver nanoparticles (AgNPs), which endowed it with antimicrobial activity that was demonstrated when challenged against Escherichia coli and methicillin-resistant Staphylococcus aureus (MRSA) on in vitro conditions.
Entropy-Based Hybrid Integration of Random Forest and Support Vector Machine for Landslide Susceptibility Analysis
Authors: Sharma Amol , Prakash Chander , Manivasagam V.
Published: October 2021

Landslide susceptibility mapping is a crucial step in comprehensive landslide risk management. The purpose of the present study is to analyze the landslide susceptibility of Mandi district, Himachal Pradesh, India, based on optimum feature selection and hybrid integration of the Shannon entropy (SE) model with random forest (RF) and support vector machine (SVM) models. An inventory of 1723 rainfall-induced landslides was generated and randomly selected for training (1199; 70%) and validation (524; 30%) purposes. A set of 14 relevant factors was selected and checked for multicollinearity. These factors were first ranked using Information Gain and Chi-square feature ranking algorithms. Furthermore, Wilcoxon Signed Rank Test and One-Sample T-Test were applied to check their statistical significance. An optimum subset of 11 landslide causative factors was then used for generating landslide susceptibility maps (LSM) using hybrid SE-RF and SE-SVM models. These LSM’s were validated and compared using receiver operating characteristic (ROC) curves and performance matrices. The SE-RF performed better with training and validation accuracies of 96.93% and 88.94%, respectively, compared with the SE-SVM model with training and validation accuracies of 94.05% and 82.4%, respectively. The prediction matrices also confirmed that the SE-RF model is better and is recommended for the landslide susceptibility analysis of similar mountainous regions worldwide.
The Cringe and the Sneer: Structures of Feeling in Veep
Authors: Kanzler Katja
Published: October 2021

This article approaches cringe comedy through the lens of its affectivity, of the somatic experiences through which it puts its audiences’ bodies, and it uses this as a point of departure to think about the genre’s cultural work. Based on the observation that no cringe comedy makes its viewers cringe for the whole duration of its storytelling, the article suggests that cringe comedies thrive on destabilizing and ambiguating the affective valence of their performances of embarrassment, constantly recalibrating or muddying the distance between viewer and characters. They are marked by tipping points at which schadenfreude and other types of humor tip into cringe, and reversely, at which cringe tips into something else. The article focuses on one of these other affective responses, which it proposes to describe as the sneer. It uses the HBO-series Veep as a case study to explore how cringe and sneer aesthetics are interlaced in an exemplary comedy, and how they fuel this particular comedy’s satiric work.
The Association between Social Support and Cognitive Impairment among the Urban Elderly in Jinan, China
Authors: Jiang Feng , Kong Fanlei , Li Shixue
Published: October 2021

China is currently facing a severe challenge of population ageing. However, no study has specifically explored the association between social support and cognitive impairment in Chinese urban elderly aged 60 and older. We explored the prevalence of cognitive impairment and its relationship with social support among the urban elderly aged above 60 years in Jinan, China. A total of 522 urban elderly individuals were recruited using multi-stage cluster random sampling, of which 35.55% were males and 64.45% were females. The average age of all participants was 69.66 ± 8.91 years old. Social support was assessed using the Social Support Rating Scale. Cognitive status was assessed using the Mini-Mental State Examination. Data were collected through face-to-face interviews using structured questionnaires. Descriptive analysis, chi-square tests, and logistic regression analyses were conducted. After analysing the data from 512 participants, 154 (30.1%), 352 (68.8%), and 6 (1.2%) participants had high, moderate, and low levels of social support, respectively. In addition, 125 participants (24.4%) had a cognitive impairment, while the other 387 participants (75.6%) had a normal cognitive status. Binary logistic regression analyses showed that age, educational level, occupation or pre-retirement occupation, and social support were statistically associated with cognitive impairment. A significant association between social support and cognitive impairment was found among the urban elderly in Jinan, China, which provided useful information for the intervention of cognitive impairment. More attention should be paid to the social support of the urban elderly to effectively reduce the occurrence of cognitive impairment.
Shipwrecks’ Underwater Mysteries—Identifying Commonalities Out of Globally-Distributed Knowledge
Authors: Koutsi Dionisia , Stratigea Anastasia
Published: October 2021

Tourism trends towards authentic, experience-based products have brought to the forefront lesser-known destinations, e.g., small and medium-sized Mediterranean islands. These can gain competitiveness on the ground of their distinguished land and Underwater Cultural Heritage (UCH), thus opening up new directions for their future sustainable development. Sustainable exploitation of UCH, though, calls for dedicated planning endeavors and respective UCH data acquisition/management. However, while the planning discipline can offer effective approaches and tools for properly handling cultural heritage, a deficit of sufficient, pertinent and well-documented UCH data is noticed, e.g., data on shipwrecks as part of UCH. The latter is the focus of this work, aiming, as a first step, to illuminate aspects of a shipwreck database, relevant to planners’ requests. Towards this end, global-wide distributed knowledge is explored in an effort to identify potentialities and limitations, content commonalities, shipwreck attributes so far recorded, (spatial) planning requirements, to name but a few. Knowledge acquired from this exploration can feed conceptualization of a planning-oriented shipwreck database. This can be replicable to various national/regional contexts; and is capable of providing well-structured shipwrecks’ content that can steer strategic planning efforts towards authentic ensembles of people’s history and culture in remote and less-privileged Mediterranean islands.
Hydrogen Sorption Properties of a Novel Refractory Ti-V-Zr-Nb-Mo High Entropy Alloy
Authors: Bouzidi Anis , Laversenne Laetitia , Zepon Guilherme , Vaughan Gavin , Nassif Vivian , Zlotea Claudia
Published: October 2021

High entropy alloys belong to a new and promising class of functional materials for solid-state hydrogen storage. In this context, a novel single-phase body centered cubic (bcc) high entropy alloy Ti0.30V0.25Zr0.10Nb0.25Mo0.10 was prepared. The physicochemical and hydrogen sorption properties have been determined by both laboratory and large-scale facilities. This alloy can quickly absorb hydrogen up to 2.0 H/M (2.8 wt.%) at room temperature and forms a face centered cubic (fcc) hydride, as proven by synchrotron X-ray diffraction. The Pressure–Composition Isotherm and in situ neutron diffraction during hydrogen/deuterium desorption reaction suggest that the alloy experiences a reversible single step phase transition (bcc↔fcc). PDF analysis from X-ray total scattering data points out that the hydride phase possesses an average fcc structure with random atoms distribution and small lattice distortion. Despite an initial small fading of the capacity, the alloy withstands 20 absorption/desorption cycling without phase decomposition, as demonstrated by kinetic measurements coupled with X-ray diffraction and microstructural study by SEM-EDS. Moreover, the complete hydrogen absorption occurs in less than 30 s at room temperature and the kinetic improves during cycling.
A Comparison of Bayesian Spatial Models for HIV Mapping in South Africa
Authors: Ayalew Kassahun Abere , Manda Samuel , Cai Bo
Published: October 2021

Despite making significant progress in tackling its HIV epidemic, South Africa, with 7.7 million people living with HIV, still has the biggest HIV epidemic in the world. The Government, in collaboration with developmental partners and agencies, has been strengthening its responses to the HIV epidemic to better target the delivery of HIV care, treatment strategies and prevention services. Population-based household HIV surveys have, over time, contributed to the country’s efforts in monitoring and understanding the magnitude and heterogeneity of the HIV epidemic. Local-level monitoring of progress made against HIV and AIDS is increasingly needed for decision making. Previous studies have provided evidence of substantial subnational variation in the HIV epidemic. Using HIV prevalence data from the 2016 South African Demographic and Health Survey, we compare three spatial smoothing models, namely, the intrinsically conditionally autoregressive normal, Laplace and skew-t (ICAR-normal, ICAR-Laplace and ICAR-skew-t) in the estimation of the HIV prevalence across 52 districts in South Africa. The parameters of the resulting models are estimated using Bayesian approaches. The skewness parameter for the ICAR-skew-t model was not statistically significant, suggesting the absence of skewness in the HIV prevalence data. Based on the deviance information criterion (DIC) model selection, the ICAR-normal and ICAR-Laplace had DIC values of 291.3 and 315, respectively, which were lower than that of the ICAR-skewed t (348.1). However, based on the model adequacy criterion using the conditional predictive ordinates (CPO), the ICAR-skew-t distribution had the lowest CPO value. Thus, the ICAR-skew-t was the best spatial smoothing model for the estimation of HIV prevalence in our study.
Precarious Employment and Psychosocial Hazards: A Cross-Sectional Study in Stockholm County
Authors: Kvart Signild , Jonsson Johanna , Bodin Theo , Håkansta Carin , Kreshpaj Bertina , Orellana Cecilia , Östergren Per-Olof , Nylén Lotta , Matilla-Santander Nuria
Published: October 2021

Precarious employment (PE) has been linked to adverse health effects, possibly mediated through psychosocial hazards. The aim of this cross-sectional study is to explore if higher levels of PE are associated with psychosocial hazards (experiences of violence, sexual harassment, bullying, discrimination, high demands, and low control) and to explore gender differences in these patterns. The study is based on survey- and register data from a sample of 401 non-standard employees in Stockholm County (2016–2017). The level of PE (low/high) was assessed with the Swedish version of the employment precariousness scale (EPRES-Se) and analysed in relation to psychosocial hazards by means of generalized linear models, with the Poisson family and robust variances. After controlling for potential confounders (gender, age, country of birth, and education), the prevalence of suffering bullying (PR 1.07, 95% CI: 1.01–1.13) and discrimination (PR 1.52, 95% CI: 1.00–2.32) was higher among individuals with a high level of PE. Regarding the demand/control variables, a high level of PE was also associated with low control (PR 1.59, 95% CI: 1.30–1.96) and passive work (the combination of low demands and low control) (PR 1.60, 95% CI: 1.23–2.08). Our findings suggest that workers in PE are more likely to experience psychosocial hazards, and these experiences are more prevalent among women compared to men. Future longitudinal studies should look further into these associations and their implications for health and health inequalities.
Healthcare Professionals’ Application and Integration of Physical Activity in Routine Practice with Older Adults: A Qualitative Study
Authors: Cunningham Conor , O’Sullivan Roger
Published: October 2021

Healthcare professionals (HCPs) have a key role in promoting physical activity, particularly among populations at greatest risk of poor health due to physical inactivity. This research explored HCPs’ knowledge, decision making, and routine practice of physical activity promotion with older adults. Furthermore, it aimed to enhance our understanding of the supports that HCPs need to effectively promote physical activity in routine practice across a wide range of healthcare professions, settings, and sectors. Semi-structured online interviews were completed with HCPs between November 2020–March 2021. Data were first analysed by coding instances within the transcripts, mapping onto relevant Theoretical Domains Framework (TDF) domains utilising a deductive thematic analysis approach. The data were then analysed utilising an inductive approach to thematically generate explanatory subthemes within the identified domains. Participants (n = 63) included general practitioners (15.87%), occupational therapists (30.16%), physiotherapists (38.10%), and nurses (15.87%) from the island of Ireland (Ireland and Northern Ireland). Of those interviewed, 10 (15.87%) were male and 53 (84.13%) were female. Two thirds (65.08%) were HCPs practicing in Ireland. Domains and subthemes related to the application of physical activity, and emergent themes on developing practice to support the application and integration of physical activity in routine practice are discussed. HCPs identified that focused education, appropriate training, and access to tailored resources are all essential to support the promotion of physical activity in routine practice. For such supports to be effective, a ‘cultural shift’ is required in HCP training and health service provision to adopt the growing evidence base that physical activity promotion must be part of disease prevention and treatment in routine practice. HCPs highlighted a range of areas for service development to support them to promote physical activity. Further research is required to explore the feasibility of implementing these recommendations in routine practice.
Relationship between Residential Segregation, Later-Life Cognition, and Incident Dementia across Race/Ethnicity
Authors: Pohl Daniel J. , Seblova Dominika , Avila Justina F. , Dorsman Karen A. , Kulick Erin R. , Casey Joan A. , Manly Jennifer
Published: October 2021

Systemic racism leads to racial/ethnic residential segregation, which can result in health inequities. We examined if the associations between residential segregation and later-life cognition and dementia differed based on segregation measure and by participant race/ethnicity. Tests of memory (n = 4616), language (n = 4333), visuospatial abilities (n = 4557), and incident dementia (n = 4556) were analyzed in older residents of Northern Manhattan, New York (mean age: 75.7 years). Segregation was measured at the block group-level using three indices: dissimilarity, isolation, and interaction. We fit multilevel linear or Cox proportional hazards models and included a race/ethnicity × segregation term to test for differential associations, adjusting for socioeconomic and health factors. Living in block groups with higher proportions of minoritized people was associated with −0.05 SD lower language scores. Living in block groups with higher potential contact between racial/ethnic groups was associated with 0.06–0.1 SD higher language scores. The findings were less pronounced for other cognitive domains and for incident dementia. Non-Hispanic Black adults were most likely to experience negative effects of neighborhood segregation on cognition (language and memory) and dementia. All indices partly capture downstream effects of structural racism (i.e., unequal distributions of wealth/resources) on cognition. Therefore, desegregation and equitable access to resources have the potential to improve later-life cognitive health.
Heavy Metal Pollution and Potential Ecological Risk Assessment in a Typical Mariculture Area in Western Guangdong
Authors: He Ning , Liu Lanzhou , Wei Ren , Sun Kaifeng
Published: October 2021

The distribution characteristics, environmental contamination states, and potential ecological risks of chromium (Cr), copper (Cu), arsenic (As), lead (Pb), and cadmium (Cd) in seawater, sediment and breeding feed were studied in a typical mariculture area in western Guangdong of China. Pearson correlation analysis was used to determine metal homology, and the single-factor index, potential ecological risk index, hazard quotient (HQ), and joint probability curve (JPC) were used to evaluate pollution states and ecological risk of metals. Four main statements can be concluded from the results: (1) Pb and Cu showed a similar distribution pattern in the seawater and sediment and their contents in the breeding wastewater exceeded the standard limits in several stations. (2) Cr, Cu, and As have similar sources in the feeds, which may be an important source of metals in water. (3) The risk assessment revealed that the sediment from the studied areas was at a low ecological risk of heavy metal, whereas, water in the pond and outfall was slightly polluted by Pb, and water in the cages and outfall were slightly polluted by Cu. (4) Both the hazard quotient (HQ) and joint probability curve showed the overall risk probabilities (ORPs) in the waters ranked as Cu > Cr > Pb > Cd > As. Although Pb and Cd had HQ values greater than 1, their ORPs were acceptable. This study highlights that multiple evaluation models are more reliable than the single ecological risk assessment for evaluating heavy metal pollution risks in the mariculture area.
Consequences of COVID-19 Confinement for Teachers: Family-Work Interactions, Technostress, and Perceived Organizational Support
Authors: Solís García Patricia , Lago Urbano Rocío , Real Castelao Sara
Published: October 2021

The confinement experienced due to the COVID-19 pandemic has prompted a rethink of the teaching–learning process to which teachers have responded without planning and instead used their resources. This study aimed to analyze the relationships between work–family interactions, technostress, and perceived organizational support in teachers during the confinement period in Spain that began in March 2020. An online survey was administered to 640 pre-school, primary, and secondary school teachers. Positive reciprocal work–family interactions and their relationship with organizational support were found, with differences according to gender, with women showing a more negative perception of the impact on the family. There were no marked levels of technostress in the overall sample, although higher levels of perceived ineffectiveness and skepticism were found in teachers aged 46 years or older. Teachers in private and subsidized schools showed a higher level of perceived support than those in public schools. There is a need to continue this work to verify the values of these dimensions in other contexts and to apply institutional measures and public policies to improve these indicators in this group.
Risk Assessment of Different Maize (Zea mays L.) Lodging Types in the Northeast and the North China Plain Based on a Joint Probability Distribution Model
Authors: Zan Xuli , Xing Ziyao , Gao Xiang , Liu Wei , Zhang Xiaodong , Liu Zhe , Li Shaoming
Published: October 2021

Mastering the lodging risk of planting environment is of great significance to the optimal layout of maize varieties and the breeding of lodging resistant varieties. However, the existing lodging risk models are still at the stage of single or multi-factors independent analysis, and lack of assessment for different lodging types. To address this issue, based on the mechanism of different lodging types, the Archimedean copula function was used to describe the joint probability distribution of wind speed and precipitation, and the lodging risk assessment model of maize was established. By comparing the goodness of fit, when the rank correlation coefficient of these two is positive and negative, the corresponding optimal joint probability distribution functions are the Gumbel copula and Frank copula. According to the spatial distribution of lodging risk, the area from Liaodong Bay northward to Tongyu, Jilin province in the Northeast and the North China Plain has a high frequency of lodging, in which the probability of stalk lodging is two to four times that of root lodging. Finally, we discussed how to apply the lodging risk distribution results to optimize the maize variety test sites to improve the efficiency and reliability of the existing test system. The method proposed in this paper comprehensively considers the synergistic effect of multiple factors and can provide technical support for other risk assessment.
Correction: Nguyen, K.A.; Chen, W. DEM- and GIS-Based Analysis of Soil Erosion Depth Using Machine Learning. ISPRS Int. J. Geo-Inf. 2021, 10, 452
Authors: Nguyen Kieu Anh , Chen Walter
Published: October 2021

The authors of the published paper [1] would like to make the following corrections:(1)The last four numbers in the second column (No [...]
Structural and Functional Analysis of Female Sex Hormones against SARS-CoV-2 Cell Entry
Authors: Aguilar-Pineda Jorge Alberto , Albaghdadi Mazen , Jiang Wanlin , Vera-Lopez Karin J. , Nieto-Montesinos Rita , Alvarez Karla Lucia F. , Davila Del-Carpio Gonzalo , Gómez Badhin , Lindsay Mark E. , Malhotra Rajeev , Lino Cardenas Christian L.
Published: October 2021

Emerging evidence suggests that males are more susceptible to severe infection by the SARS-CoV-2 virus than females. A variety of mechanisms may underlie the observed gender-related disparities including differences in sex hormones. However, the precise mechanisms by which female sex hormones may provide protection against SARS-CoV-2 infectivity remains unknown. Here we report new insights into the molecular basis of the interactions between the SARS-CoV-2 spike (S) protein and the human ACE2 receptor. We further report that glycosylation of the ACE2 receptor enhances SARS-CoV-2 infectivity. Importantly, estrogens can disrupt glycan–glycan interactions and glycan–protein interactions between the human ACE2 and the SARS-CoV-2 thereby blocking its entry into cells. In a mouse model of COVID-19, estrogens reduced ACE2 glycosylation and thereby alveolar uptake of the SARS-CoV-2 spike protein. These results shed light on a putative mechanism whereby female sex hormones may provide protection from developing severe infection and could inform the development of future therapies against COVID-19.
Prediction of Anti-Glioblastoma Drug-Decorated Nanoparticle Delivery Systems Using Molecular Descriptors and Machine Learning
Authors: Munteanu Cristian R. , Gutiérrez-Asorey Pablo , Blanes-Rodríguez Manuel , Hidalgo-Delgado Ismael , Blanco Liverio María de Jesús , Castiñeiras Galdo Brais , Porto-Pazos Ana B. , Gestal Marcos , Arrasate Sonia , González-Díaz Humbert
Published: October 2021

The theoretical prediction of drug-decorated nanoparticles (DDNPs) has become a very important task in medical applications. For the current paper, Perturbation Theory Machine Learning (PTML) models were built to predict the probability of different pairs of drugs and nanoparticles creating DDNP complexes with anti-glioblastoma activity. PTML models use the perturbations of molecular descriptors of drugs and nanoparticles as inputs in experimental conditions. The raw dataset was obtained by mixing the nanoparticle experimental data with drug assays from the ChEMBL database. Ten types of machine learning methods have been tested. Only 41 features have been selected for 855,129 drug-nanoparticle complexes. The best model was obtained with the Bagging classifier, an ensemble meta-estimator based on 20 decision trees, with an area under the receiver operating characteristic curve (AUROC) of 0.96, and an accuracy of 87% (test subset). This model could be useful for the virtual screening of nanoparticle-drug complexes in glioblastoma. All the calculations can be reproduced with the datasets and python scripts, which are freely available as a GitHub repository from authors.
Disentangling Mitochondria in Alzheimer’s Disease
Authors: Johri Ashu
Published: October 2021

Alzheimer’s disease (AD) is a major cause of dementia in older adults and is fast becoming a major societal and economic burden due to an increase in life expectancy. Age seems to be the major factor driving AD, and currently, only symptomatic treatments are available. AD has a complex etiology, although mitochondrial dysfunction, oxidative stress, inflammation, and metabolic abnormalities have been widely and deeply investigated as plausible mechanisms for its neuropathology. Aβ plaques and hyperphosphorylated tau aggregates, along with cognitive deficits and behavioral problems, are the hallmarks of the disease. Restoration of mitochondrial bioenergetics, prevention of oxidative stress, and diet and exercise seem to be effective in reducing Aβ and in ameliorating learning and memory problems. Many mitochondria-targeted antioxidants have been tested in AD and are currently in development. However, larger streamlined clinical studies are needed to provide hard evidence of benefits in AD. This review discusses the causative factors, as well as potential therapeutics employed in the treatment of AD.
Simple Does Not Mean Trivial: Behavior of Phosphatidic Acid in Lipid Mono- and Bilayers
Authors: Drabik Dominik , Czogalla Aleksander
Published: October 2021

Phosphatidic acid (PA) is one of the simplest membrane phospholipids, yet it plays a crucial role in various biologically relevant processes that take place in cells. Since PA generation may be triggered by a variety of factors, very often of antagonistic character, the specific nature of physiological responses driven by PA is not clear. In order to shed more light on these issues, we carried out a systematic characterization of membranes containing one of the three biologically significant PA molecular species. The effect of these molecules on the properties of membranes composed of phosphatidylcholine and/or cholesterol was assessed in a multidisciplinary approach, including molecular dynamic simulations, flicker noise spectroscopy, and Langmuir monolayer isotherms. The first enables the determination of various macroscopic and microscopic parameters such as lateral diffusion, membrane thickness, and defect analysis. The obtained data revealed a strong interaction between unsaturated PA species and phosphatidylcholine. On the other hand, the behavior of saturated PA was greatly influenced by cholesterol. Additionally, a strong effect on mechanical properties was observed in the case of three-component systems, which could not be explained by the simple extrapolation of parameters of the corresponding two-component systems. Our data show that various PA species are not equivalent in terms of their influence on lipid mono- and bilayers and that membrane composition/properties, particularly those related to the presence of cholesterol, may strongly modulate PA behavior.
The Bioactivity of Thiazolidin-4-Ones: A Short Review of the Most Recent Studies
Authors: Mech Dominika , Kurowska Antonina , Trotsko Nazar
Published: October 2021

Thiazolidin-4-ones is an important heterocyclic ring system of a pharmacophore and a privileged scaffold in medicinal chemistry. This review is focused on the latest scientific reports regarding biological activities of thiazolidin-4-ones published in 2020 and 2021. The review covers recent information about antioxidant, anticancer, anti-inflammatory, analgesic, anticonvulsant, antidiabetic, antiparasitic, antimicrobial, antitubercular and antiviral properties of thiazolidin-4-ones. Additionally, the influence of different substituents in molecules on their biological activity was discussed in this paper. Thus, this study may help to optimize the structure of thiazolidin-4-one derivatives as more efficient drug agents. Presented information may be used as a practical hint for rational design of new small molecules with biological activity, especially among thiazolidin-4-ones.
Enhanced Resistance of atbzip62 against Pseudomonas syringae pv. tomato Suggests Negative Regulation of Plant Basal Defense and Systemic Acquired Resistance by AtbZIP62 Transcription Factor
Authors: Nabi Rizwana Begum Syed , Rolly Nkulu Kabange , Tayade Rupesh , Khan Murtaza , Shahid Muhammad , Yun Byung-Wook
Published: October 2021

The intrinsic defense mechanisms of plants toward pathogenic bacteria have been widely investigated for years and are still at the center of interest in plant biosciences research. This study investigated the role of the AtbZIP62 gene encoding a transcription factor (TF) in the basal defense and systemic acquired resistance in Arabidopsis using the reverse genetics approach. To achieve that, the atbzip62 mutant line (lacking the AtbZIP62 gene) was challenged with Pseudomonas syringae pv. tomato (Pst DC3000) inoculated by infiltration into Arabidopsis leaves at the rosette stage. The results indicated that atbzip62 plants showed an enhanced resistance phenotype toward Pst DC3000 vir over time compared to Col-0 and the susceptible disease controls, atgsnor1-3 and atsid2. In addition, the transcript accumulation of pathogenesis-related genes, AtPR1 and AtPR2, increased significantly in atbzip62 over time (0–72 h post-inoculation, hpi) compared to that of atgsnor1-3 and atsid2 (susceptible lines), with AtPR1 prevailing over AtPR2. When coupled with the recorded pathogen growth (expressed as a colony-forming unit, CFU mL−1), the induction of PR genes, associated with the salicylic acid (SA) defense signaling, in part explained the observed enhanced resistance of atbzip62 mutant plants in response to Pst DC3000 vir. Furthermore, when Pst DC3000 avrB was inoculated, the expression of AtPR1 was upregulated in the systemic leaves of Col-0, while that of AtPR2 remained at a basal level in Col-0. Moreover, the expression of AtAZI (a systemic acquired resistance -related) gene was significantly upregulated at all time points (0–24 h post-inoculation, hpi) in atbzip62 compared to Col-0 and atgsnor1-3 and atsid2. Under the same conditions, AtG3DPH exhibited a high transcript accumulation level 48 hpi in the atbzip62 background. Therefore, all data put together suggest that AtPR1 and AtPR2 coupled with AtAZI and AtG3DPH, with AtAZI prevailing over AtG3DPH, would contribute to the recorded enhanced resistance phenotype of the atbzip62 mutant line against Pst DC3000. Thus, the AtbZIP62 TF is proposed as a negative regulator of basal defense and systemic acquired resistance in plants under Pst DC3000 infection.
The Next Generation of Molecular and Cellular Therapeutics for Inherited Retinal Disease
Authors: Martinez Velazquez Luis A. , Ballios Brian G.
Published: October 2021

Inherited retinal degenerations (IRDs) are a diverse group of conditions that are often characterized by the loss of photoreceptors and blindness. Recent innovations in molecular biology and genomics have allowed us to identify the causative defects behind these dystrophies and to design therapeutics that target specific mechanisms of retinal disease. Recently, the FDA approved the first in vivo gene therapy for one of these hereditary blinding conditions. Current clinical trials are exploring new therapies that could provide treatment for a growing number of retinal dystrophies. While the field has had early success with gene augmentation strategies for treating retinal disease based on loss-of-function mutations, many novel approaches hold the promise of offering therapies that span the full spectrum of causative mutations and mechanisms. Here, we provide a comprehensive review of the approaches currently in development including a discussion of retinal neuroprotection, gene therapies (gene augmentation, gene editing, RNA modification, optogenetics), and regenerative stem or precursor cell-based therapies. Our review focuses on technologies that are being developed for clinical translation or are in active clinical trials and discusses the advantages and limitations for each approach.
Aberrant Mitochondrial Dynamics and Exacerbated Response to Neuroinflammation in a Novel Mouse Model of CMT2A
Authors: Stavropoulos Filippos , Sargiannidou Irene , Potamiti Louiza , Kagiava Alexia , Panayiotidis Mihalis I. , Bae Ji Hyun , Yeom Su Cheong , Lee Jae Young , Kleopa Kleopas A.
Published: October 2021

Charcot-Marie-Tooth disease type 2A (CMT2A) is the most common hereditary axonal neuropathy caused by mutations in MFN2 encoding Mitofusin-2, a multifunctional protein located in the outer mitochondrial membrane. In order to study the effects of a novel MFN2K357T mutation associated with early onset, autosomal dominant severe CMT2A, we generated a knock-in mouse model. While Mfn2K357T/K357T mouse pups were postnatally lethal, Mfn2+/K357T heterozygous mice were asymptomatic and had no histopathological changes in their sciatic nerves up to 10 months of age. However, immunofluorescence analysis of Mfn2+/K357T mice revealed aberrant mitochondrial clustering in the sciatic nerves from 6 months of age, in optic nerves from 8 months, and in lumbar spinal cord white matter at 10 months, along with microglia activation. Ultrastructural analyses confirmed dysmorphic mitochondrial aggregates in sciatic and optic nerves. After exposure of 6-month-old mice to lipopolysaccharide, Mfn2+/K357T mice displayed a higher immune response, a more severe motor impairment, and increased CNS inflammation, microglia activation, and macrophage infiltrates. Overall, ubiquitous Mfn2K357T expression renders the CNS and peripheral nerves of Mfn2+/K357T mice more susceptible to mitochondrial clustering, and augments their response to inflammation, modeling some cellular mechanisms that may be relevant for the development of neuropathy in patients with CMT2A.
Parametric Open Source Cold-Frame Agrivoltaic Systems
Authors: Pearce Joshua M.
Published: October 2021

There is an intense need to optimize agrivoltaic systems. This article describes the invention of a new testing system: the parametric open source cold-frame agrivoltaic system (POSCAS). POSCAS is an adapted gardening cold-frame used in cold climates as it acts as a small greenhouse for agricultural production. POSCAS is designed to test partially transparent solar photovoltaic (PV) modules targeting the agrivoltaic market. It can both function as a traditional cold frame, but it can also be automated to function as a full-service greenhouse. The integrated PV module roof can be used to power the controls or it can be attached to a microinverter to produce power. POSCAS can be placed in an experimental array for testing agricultural and power production. It can be easily adapted for any type of partially transparent PV module. An array of POSCAS systems allows for the testing of agrivoltaic impacts from the percent transparency of the modules by varying the thickness of a thin film PV material or the density of silicon-based cells, and various forms of optical enhancement, anti-reflection coatings and solar light spectral shifting materials in the back sheet. All agrivoltaic variables can be customized to identify ideal PV designs for a given agricultural crop.
Blood Differential Gene Expression in Patients with Chronic Heart Failure and Systemic Iron Deficiency: Pathways Involved in Pathophysiology and Impact on Clinical Outcomes
Authors: Díez-López Carles , Tajes Orduña Marta , Enjuanes Grau Cristina , Moliner Borja Pedro , González-Costello José , García-Romero Elena , Francesch Manzano Josep , Yun Viladomat Sergi , Jiménez-Marrero Santiago , Ramos-Polo Raul , Ras Jiménez Maria del Mar , Comín-Colet Josep
Published: October 2021

Background: Iron deficiency is a common disorder in patients with heart failure and is related with adverse outcomes and poor quality of life. Previous experimental studies have shown biological connections between iron homeostasis, mitochondrial metabolism, and myocardial function. However, the mechanisms involved in this crosstalk are yet to be unfolded. Methods: The present research attempts to investigate the intrinsic biological mechanisms between heart failure and iron deficiency and to identify potential prognostic biomarkers by determining the gene expression pattern in the blood of heart failure patients, using whole transcriptome and targeted TaqMan® low-density array analyses. Results: We performed a stepwise cross-sectional longitudinal study in a cohort of chronic heart failure patients with and without systemic iron deficiency. First, the full transcriptome was performed in a nested case-control exploratory cohort of 7 paired patients and underscored 1128 differentially expressed transcripts according to iron status (cohort1#). Later, we analyzed the messenger RNA levels of 22 genes selected by their statistical significance and pathophysiological relevance, in a validation cohort of 71 patients (cohort 2#). Patients with systemic iron deficiency presented lower mRNA levels of mitochondrial ferritin, sirtuin-7, small integral membrane protein 20, adrenomedullin and endothelin converting enzyme-1. An intermediate mitochondrial ferritin gene expression and an intermediate or low sirtuin7 and small integral membrane protein 20 mRNA levels were associated with an increased risk of all-cause mortality and heart failure admission ((HR 2.40, 95% CI 1.04–5.50, p-value = 0.039), (HR 5.49, 95% CI 1.78–16.92, p-value = 0.003), (HR 9.51, 95% CI 2.69–33.53, p-value < 0.001), respectively). Conclusions: Patients with chronic heart failure present different patterns of blood gene expression depending on systemic iron status that affect pivotal genes involved in iron regulation, mitochondrial metabolism, endothelial function and cardiovascular physiology, and correlate with adverse clinical outcomes.
miRNAs in the Expression Regulation of Dopamine-Related Genes and Proteins in Endometrial Cancer
Authors: Czerwiński Michał , Bednarska-Czerwińska Anna , Zmarzły Nikola , Boroń Dariusz , Oplawski Marcin , Grabarek Beniamin Oskar
Published: October 2021

Disruption of the dopaminergic system leads to many diseases, including cancer. Dopamine and its receptors are involved in the regulation of proliferation, cell death, invasion, and migration. Better understanding of the mechanisms involved in these processes could reveal new molecular markers and therapeutic targets. The aim of this study was to determine the expression profile of dopamine-related genes and proteins in endometrial cancer and to assess whether miRNAs are involved in its regulation. Sixty women were recruited for the study: 30 with endometrial cancer and 30 without cancer. The expression profiles of dopamine-related genes were determined in endometrial tissue samples using microarrays and qRT-PCR. Then, protein concentration was determined with the ELISA test. In the last step, miRNA detection was performed using microarrays. The matching of miRNAs to the studied genes was carried out using the TargetScan tool. The analysis showed DRD2 and DRD3 overexpression, with a reduction in DRD5 expression, which could be due to miR-15a-5p, miR-141-3p, miR-4640-5p, and miR-221-5p activity. High levels of OPRK1 and CXCL12, related to the activity of miR-124-3p.1 and miR-135b-5p, have also been reported. Low COMT expression was probably not associated with miRNA regulation in endometrial cancer.
Cerebellar White Matter Abnormalities in Charcot–Marie–Tooth Disease: A Combined Volumetry and Diffusion Tensor Imaging Analysis
Authors: Hwang Sungeun , Park Chang-Hyun , Kim Regina Eun-Young , Kim Hyeon Jin , Choi Yun Seo , Kim Sol-Ah , Yoo Jeong Hyun , Chung Ki Wha , Choi Byung-Ok , Lee Hyang Woon
Published: October 2021

Charcot–Marie–Tooth disease (CMT) is a genetically heterogeneous hereditary peripheral neuropathy. Brain volumetry and diffusion tensor imaging (DTI) were performed in 47 controls and 47 CMT patients with PMP22 duplication (n = 10), MFN2 (n = 15), GJB1 (n = 11), or NEFL mutations (n = 11) to investigate for structural changes in the cerebellum. Volume of cerebellar white matter (WM) was significantly reduced in CMT patients with NEFL mutations. Abnormal DTI findings were observed in the superior, middle, and inferior cerebellar peduncles, predominantly in NEFL mutations and partly in GJB1 mutations. Cerebellar ataxia was more prevalent in the NEFL mutation group (72.7%) than the GJB1 mutation group (9.1%) but was not observed in other genotypic subtypes, which indicates that structural cerebellar abnormalities were associated with the presence of cerebellar ataxia. However, NEFL and GJB1 mutations did not affect cerebellar gray matter (GM), and neither cerebellar GM nor WM abnormalities were observed in the PMP22 duplication or MFN2 mutation groups. We found structural evidence of cerebellar WM abnormalities in CMT patients with NEFL and GJB1 mutations and an association between cerebellar WM involvement and cerebellar ataxia in these genetic subtypes, especially in the NEFL subgroup. Therefore, we suggest that neuroimaging, such as MRI volumetry or DTI, for CMT patients could play an important role in detecting abnormalities of cerebellar WM.