bluuebunny/crossref_metadata_2025 huggingface dataset:
Perception of undergraduate students in speech-language pathology about speech therapy in the field of deafness
Authors: Gonçalves Renata da Silva , Lima Maria Cecília Marconi Pinheiro , Silva Angélica Bronzatto de Paiva e , Gasparetto Maria Elisabete Rodrigues Freire
Published: March 2019

ABSTRACT Objective: to know the perception of undergraduate students in speech-language pathology about speech therapies in the area of deafness. Methods: qualitative research, with data collection accomplished with a questionnaire and a focus group. The sample was accomplished by 14 students enrolled at a speech pathology course. Results: the results were discussed by means of categories: knowledge, expectations, needs, bond and therapeutic practices. The students presented feelings of anguish in dealing with family expectations, especially regarding to the speech work and the fear for intervening caused by inexperience and necessities of the family. It was also evidenced that the students hoped that family members believed in the potential of the child. There is a need for more practice in Brazilian Sign Language. The support of the family members is crucial for a good therapeutical accompaniment, because when they trust on the student, the kind of therapy, and on the adopted approach, he or she cooperates with the evolution of the therapeutical process, facilitating the bond. When the family believes in the effectiveness of the therapy and the possibility of good results, the child does not miss sessions. Moreover, the bond created with the family and the patient helps the student to see the real difficulties of the child and what should be done to be improved. It was pointed out that the bilingual approach cooperates for the self-recognition of deaf culture and identity. Conclusion: on students' perception, the experience gained along the stage provided theoretical-practical knowledge to work with the family, recognizing the importance of this to the therapeutic success and to deal with the expectations of both parties.
Brazilian legislation and the school inclusion of individuals with communication disorders
Authors: Godoy Vanessa Boldarini de , Faiad Lia Netto Vaz , Machado Maria Aparecida Miranda de Paula , Crenitte Patrícia de Abreu Pinheiro , Lamônica Dionísia Aparecida Cusin , Hage Simone Rocha de Vasconcellos
Published: March 2019

ABSTRACT Objective: to verify if the current Brazilian Legislation assures the effective school inclusion of individuals with communication disorders. Methods: the present study is an integrative review. Legislation related to the topic was searched on public databases, such as the website of the Planalto Palace (Brazilian Federal government headquarters), the website of the Culture and Education Ministry (Ministério de Educação e Cultura - MEC) the website of the National Education Council from Federal Government. Brazilian laws, ordinances and guidelines regarding special education of individuals with autism, intellectual disability, hearing impairment and dyslexia were consulted, since the descriptor "Communication Disorders" did not show any results. Results: twenty-one (21) identified norms met the inclusion criteria. Among these, thirteen (13) address disabilities in a generalized manner. The other eight (8) regulations are specific in relation to autism, intellectual disability, hearing impairment and dyslexia. Conclusion: despite the significant number of norms, the current Brazilian legislation does not allow for real school inclusion. Individuals with communication disorders do not have a specific legislation that could guide the peculiarities of each disorder, as well as their special educational needs. Thus, it was concluded that these individuals need legal support respecting their specificities, to allow their effective school inclusion.
Qualidade de vida e excesso de peso em trabalhadores em turnos alternantes
Authors: Pimenta Fausto Aloísio Pedrosa , Alves Rafaella Lemos , Oliveira Fernando Luiz Pereira de , Nascimento Neto Raimundo Marques do , Coelho George Luiz Lins Machado , Freitas Silvia Nascimento de
Published: March 2019

Resumo Objetivo: identificar a percepção dos trabalhadores de turnos alternantes de uma mineradora da região dos Inconfidentes, Minas Gerais, sobre sua qualidade de vida (QV) e analisar sua associação com indicadores de excesso de adiposidade corporal. Métodos: estudo transversal com 437 trabalhadores em turnos alternantes com um ou mais fatores de risco cardiovascular. Dados sociodemográficos e clínicos foram analisados. A QV foi avaliada com o questionário SF-36 e a adiposidade corporal estimada a partir das medidas antropométricas e de composição corporal. Resultados: a pontuação para os domínios da QV variou de 67 a 100, no entanto, o aumento de gordura corporal apresentou correlação negativa com os domínios saúde geral, vitalidade e capacidade funcional. Foi observada, por análise de cluster, a formação de dois agrupamentos, um composto pelos domínios da QV e o outro constituído pelos indicadores de composição corporal. Não foi identificada associação entre os escores estimados de QV dos trabalhadores de turno e o tempo de trabalho. Conclusão: o declínio da QV apresentou associação com o excesso de adiposidade corporal. Recomenda-se a adoção de medidas visando reduzir o excesso de adiposidade corporal e melhorar a qualidade de vida dos trabalhadores em turno alternante da mineração.
Programas de atenção a homens autores de violência contra as mulheres: um panorama das intervenções no Brasil
Authors: Beiras Adriano , Nascimento Marcos , Incrocci Caio
Published: March 2019

Resumo A violência contra mulheres é considerada um grave problema de saúde pública, uma violação dos direitos em todo o mundo. Como parte das políticas de enfrentamento a esse tipo de violência, a Lei Maria da Penha prevê a participação de homens autores de violência (HAV) em programas de atenção cuja atuação principal é a realização de grupos reflexivos. Este artigo apresenta um mapeamento de programas para HAV no Brasil realizado entre 2015 e 2016. Trata-se de uma pesquisa qualitativa, exploratória e descritiva realizada em dois momentos. No primeiro, foram localizados 41 programas das cinco regiões do país, dos quais 26 responderam um questionário com perguntas abertas e fechadas. Os dados foram analisados segundo quatro categorias: estrutura do programa, metodologia empregada, monitoramento e avaliação e resultados e desafios. No segundo momento, foram analisados outros mapeamentos de programas brasileiros e internacionais com HAV, além de documentos nacionais e internacionais que sugerem diretrizes para sua execução. Notou-se semelhanças entre os programas encontrados e os documentos analisados, como o vínculo das iniciativas com instâncias governamentais, fundamentação nas teorias de gênero e modalidade de intervenções em grupo. A partir do diálogo entre outros mapeamentos, estudos apresentados e resultados encontrados, sugere-se diretrizes mínimas para a realização de grupos reflexivos.
Melhor no passado: a verdadeira saúde da família
Authors: Valadão Patrícia Aparecida da Silva , Lins Liliane , Carvalho Fernando Martins
Published: March 2019

Resumo Buscou-se avaliar os limites da Estratégia Saúde da Família (ESF) a partir do cotidiano de trabalho de seus profissionais. Neste estudo qualitativo, realizaram-se entrevistas individuais semiestruturadas com 16 profissionais da equipe de Saúde da Família de um município da região metropolitana de Salvador, Bahia. O exame dos dados utilizou a análise de conteúdo proposta por Bardin e os referenciais da Política Nacional de Atenção Básica e da Política Nacional de Humanização para Atenção Básica. Identificaram-se duas categorias: a atenção básica (AB) do Sistema Único de Saúde (SUS) e a cogestão e humanização na AB. Na primeira categoria, evidenciou-se o contexto atual que permeia a AB, caracterizando os desafios que se apresentam no cotidiano de trabalho dos profissionais da ESF e as dificuldades encontradas para mudar o modelo de atenção à saúde. Na segunda categoria, destacaram-se as condições concretas em que se realizam as práticas no cotidiano de trabalho, indo de encontro aos pressupostos da humanização. Os problemas apresentados evidenciam descaracterização da AB, contradizem os preceitos da ESF e revelam dificuldades na proposta de reorientação do modelo de saúde.
Mal-estar, sofrimento e adoecimento do professor: de narrativas do trabalho e da cultura docente à docência como profissão
Authors: Penteado Regina Zanella , Souza Neto Samuel de
Published: March 2019

Resumo Este trabalho tem como objetivo apresentar uma leitura crítica da problemática do mal-estar, dos sofrimentos e dos adoecimentos de professores, vinculada à história do trabalho docente, aos modos de ser/estar na ocupação e à cultura do magistério. A metodologia envolveu revisão narrativa de 12 publicações selecionadas, o que possibilitou identificar os principais aspectos atribuídos às questões: epidemiológicos, de naturalização da problemática na docência, políticas públicas, organização do trabalho docente e identidade profissional docente. A discussão busca apontar as formas de visibilidade e invisibilidade dessa problemática no cenário social e educacional brasileiro, com atenção para o trabalho, a formação de professores e a cultura do magistério mediante os aportes de Maurice Tardif a respeito das “idades do ensino”. O estudo mostra como o mal-estar, o sofrimento e o adoecimento de professores podem exprimir narrativas coletivas da docência marcada pela vocação e pela socialização profissional pela feminização - e como dimensões sociais, históricas e culturais do trabalho docente podem interferir nos modos coletivos de perceber e cuidar do corpo e da saúde e levar ao adoecimento. Por fim, aponta o desafio de se pensar corpo/saúde/bem-estar no âmbito da cultura ocupacional docente, como elementos que substanciam o desenvolvimento profissional e o projeto de profissionalização docente.
“UPA é nós aqui mesmo”: as redes de apoio social no cuidado à saúde da população em situação de rua em um município de pequeno porte
Authors: Vale Aléxa Rodrigues do , Vecchia Marcelo Dalla
Published: March 2019

Resumo A população em situação de rua (PSR) constitui-se como um público crescente nas cidades, refletindo processos sociais desiguais e excludentes. Entre as barreiras para o acesso aos direitos sociais básicos, destaca-se a negação do direito à saúde a essas pessoas. Poucos estudos buscam conhecer as estratégias desenvolvidas pela PSR diante da carência de serviços públicos e das barreiras de acesso aos existentes. Diante disso, buscou-se identificar e analisar os itinerários terapêuticos da PSR em um município de pequeno porte. Foi utilizada a triangulação de métodos qualitativos para a coleta de informações por meio de observações participantes e entrevistas semiestruturadas. Foram entrevistados sete homens e uma mulher, e o conjunto de dados foi analisado com base na análise temática. Ressalta-se, nos relatos, a utilização de redes de apoio social como fonte prioritária de cuidado, por intermédio do autocuidado, da automedicação, do uso da medicina popular e da mudança na rotina da vida nas ruas para recuperação da saúde. Tais redes também são alternativos à garantia de acesso aos serviços públicos. Evidencia-se a negação do direito à saúde decorrente do processo de exclusão social a que estão submetidos. Destaca-se o compartilhamento de uma visão de saúde ampliada, relacionada aos determinantes sociais do processo saúde-doença. Diante desse panorama, é fundamental que o setor profissional de cuidado à saúde promova ações que permitam o cuidado contínuo e integral da PSR.
Problematic, disturbing and non-conforming children’s behaviors: the concepts and care demands related to agitation in children in Santos and Campinas, Brazil
Authors: Nakamura Eunice , Barbarini Tatiana
Published: March 2019

Abstract This article discusses the social consequences of the impossibility of specifically defining the boundaries of the concept of mental disorder, which seems to be a “vague” term with no satisfactory definition, especially when referred to children’s behaviors. We argue that when discussing children’s problematic, disturbing or non-conforming behaviors it is necessary to understand how these concepts are related to the classificatory categories of children’s behaviors and presented as care demands, whether in common sense or in biomedical discourses. Data were collected in qualitative research developed in three different child mental health services (CMHS), one in Santos (2012) and two in Campinas (2009-2010; 2017-2018), Brazil. Based on what seems to be a relation between biological-psychological dysfunction and social-cultural expectation or response, our starting point is that agitation is also a multidimensional and vague category, presenting a description and theoretical reflection about the various concepts regarding agitation. The analysis focuses on the different uses of the concepts of agitation; the social actors and institutions involved in care demands and how they are interdependently connected; then revealing, from a sociocultural perspective, the implications of classifying and defining children’s behavior from this vague category.
Briefing: The ICE Health and Safety Register
Authors: Edmonds Lloyd
Published: March 2019

This is a briefing note on the Institution of Civil Engineers’ Health and Safety Register and the importance of lifelong learning to practising civil engineers. It takes a look at the next steps needed if civil engineers really want to make their industry a safe and healthy place to work in.
Structural analysis and optimal design of steel lattice wind turbine towers
Authors: Stavridou Nafsika , Koltsakis Efthymios , Baniotopoulos Charalampos
Published: March 2019

Wind energy has shown its dominance among the means of sustainable energy production by the accelerating rise in total installed capacity and increase in size of wind energy structures. Taking into consideration the fact that the supporting structure of onshore wind power generators constitutes approximately one-third of the initial construction cost, structural optimisation of the tower is considered crucial towards the minimisation of capital expenditure during construction. Contemporary energy needs employ the construction of constantly taller and more powerful wind power converters, whose robust design in parallel with compression of initial capital expenditure cannot be neglected. The dominant structural configuration for onshore wind power generators is the tapered steel tower, but lattice towers using enhanced special cross-sections can be a rather promising solution towards economy of material use. The present paper addresses the structural performance and optimisation of tubular and lattice steel wind turbine towers, examining alternative configuration solutions for a given height and rotor characteristics. The finite-element software Abaqus has been used for the implementation of the structural models and an algorithm has been elaborated in Mathematica software in order to allow for optimisation of the use of the cross-section in the case of lattice towers.
Post-earthquake fire behaviour of steel-to-concrete joints using post-installed anchorages
Authors: Xie Qun , Xue Song , Xu Lei
Published: March 2019

Post-installed anchors have gained widespread application for connecting new structural steel components to existing concrete structures. However, knowledge about these anchor systems in hazardous conditions such as a post-earthquake fire (PEF) is very limited. An experimental and numerical investigation into steel-to-concrete connections using retrofitted anchorages subjected to a PEF was thus carried out. To explore the influence of different seismic damage levels, two cyclic loading protocols were adopted in pre-damage tests. Numerical analysis indicated there was a sharp reduction in residual strength after the bonded-in part of the anchor yielded. A damage-index-dependent prediction method of residual strength was developed based on the simulation results. In the subsequent fire test, three specimens were heated for 2 h in a furnace and then cooled to room temperature. The maximum temperature of the bonded-in anchor was 172°C while the peak furnace temperature was 588°C. The simulation results of thermal analysis exhibited a clear temperature gradient along the length of the anchor. Finally, a monotonic test with increasing load up to failure after fire exposure was carried out. The results showed that ductile steel failure of the anchor system could be achieved even under a PEF scenario.
The RNA-Protein Interactome of Differentiated Kidney Tubular Epithelial Cells
Authors: Ignarski Michael , Rill Constantin , Kaiser Rainer W.J. , Kaldirim Madlen , Neuhaus René , Esmaillie Reza , Li Xinping , Klein Corinna , Bohl Katrin , Petersen Maike , Frese Christian K. , Höhne Martin , Atanassov Ilian , Rinschen Markus M. , Höpker Katja , Schermer Bernhard , Benzing Thomas , Dieterich Christoph , Fabretti Francesca , Müller Roman-Ulrich
Published: March 2019

Significance Statement

RNA-binding proteins (RBPs) are crucial regulators of cellular biology, and recent evidence suggests that regulation of RBPs that modulate both RNA stability and translation may have a profound effect on the proteome. However, little is known about regulation of RBPs upon clinically relevant changes of the cellular microenvironment. The authors used high-throughput approaches to study the cellular RNA‐binding proteome in differentiated tubular epithelial cells exposed to hypoxia. They identified a number of novel RBPs (suggesting that these proteins may be specific RBPs in differentiated tubular epithelial cells), and found quantitative differences in RBP-binding to mRNA associated with hypoxia versus normoxia. These findings demonstrate the regulation of RBPs through environmental stimuli and provide insight into the biology of hypoxia-response signaling in the kidney.

Background

RNA-binding proteins (RBPs) are fundamental regulators of cellular biology that affect all steps in the generation and processing of RNA molecules. Recent evidence suggests that regulation of RBPs that modulate both RNA stability and translation may have a profound effect on the proteome. However, regulation of RBPs in clinically relevant experimental conditions has not been studied systematically.

Methods

We used RNA interactome capture, a method for the global identification of RBPs to characterize the global RNA‐binding proteome (RBPome) associated with polyA-tailed RNA species in murine ciliated epithelial cells of the inner medullary collecting duct. To study regulation of RBPs in a clinically relevant condition, we analyzed hypoxia-associated changes of the RBPome.

Results

We identified >1000 RBPs that had been previously found using other systems. In addition, we found a number of novel RBPs not identified by previous screens using mouse or human cells, suggesting that these proteins may be specific RBPs in differentiated kidney epithelial cells. We also found quantitative differences in RBP-binding to mRNA that were associated with hypoxia versus normoxia.

Conclusions

These findings demonstrate the regulation of RBPs through environmental stimuli and provide insight into the biology of hypoxia-response signaling in epithelial cells in the kidney. A repository of the RBPome and proteome in kidney tubular epithelial cells, derived from our findings, is freely accessible online, and may contribute to a better understanding of the role of RNA-protein interactions in kidney tubular epithelial cells, including the response of these cells to hypoxia.
EVDEN ÇALIŞMA: ÖZGÜRLÜK MÜ ESARET Mİ?
Authors: Kıcır Başak
Published: March 2019

Çalışma günümüzde büyük değişimden geçmekte, yeni esnek
çalışma biçimleri ortaya çıkmaktadır. Bilgisayar ve internet teknolojilerindeki
gelişmeler işin işyeri dışına taşınmasına olanak vermektedir. Evden çalışma son
20 yıl içerisinde yaygınlık kazanmıştır. Bu çalışmanın amacı evden çalışma
kararında etkili olan faktörleri, evden çalışmanın avantaj ve dezavantajlarını
belirlemektir. Evden çalışmanın bireyin sosyal yaşamına etkisi de ele
alınmıştır.Konu hakkında ayrıntılı bilgi edinmek için nitel
araştırma yöntemi benimsenmiştir. Araştırma kapsamında evden çalışan 12 yazın
çevirmeniyle yüz yüze görüşmeler yapılmıştır.

Araştırma sonucunda evden çalışan kişilerin zaman ve
mekân esnekliğine sahip olduğu ve gerektiğinde bu esneklikten faydalandıkları
tespit edilmiştir. Evden çalışan çevirmenler iş özerkliğine de sahiptir.
Çevirmenlerden bir kısmı evden çalışmanın sosyal yaşama olumlu etkisi olduğunu
dile getirirken diğer kısmı evden çalışmanın yalnızlaştırıcı etkileri olduğunu
ifade etmiştir.
BELEDİYELERİN SÜRDÜRÜLEBİLİRLİK UYGULAMALARININ TURİSTİK HİZMET KALİTESİNE ETKİSİ
Authors: KURGUN Osman Avşar , TEKİN AKTAŞ Duygu
Published: March 2019

Çalışmanın
amacı; “Belediyelerin gerçekleştirdiği sürdürülebilirlik uygulamalarının destinasyon
turistik hizmet kalitesinin artırılmasında, destinasyon ekosistemi paydaş
rollerini de dikkate alarak, etkinliğinin araştırılması” olarak belirlenmiştir.
Çalışmada ayrıca, belediyelerin sürdürülebilirlik çalışmalarında destinasyon
temelli bir ekosistem yaklaşımıyla paydaşları ile gerçekleştirdiği
işbirliklerinin yapısı da dikkate alınmaktadır. Ek olarak, söz konusu
destinasyon temelli turizm ekosistemi paydaşlarının sürdürülebilirlik
çalışmalarında oynadığı roller, bu rollerin gerek sürdürülebilirliğe gerekse
destinasyon hizmet kalitesine etkisine konuları da çalışmanın ilgi alanında
bulunmaktadır. Çalışma kapsamında elde edilen
araştırma bulguları değerlendirildiğinde belediyelerin sürdürülebilirlik
çalışmalarının turistik hizmet kalitesine etkilerine yönelik üç önemli faktör; a)Destinasyon
temelinde makro ve mikro boyutta iletişim kalitesi ve teknik birikim
b)Nitelikli personel ihtiyacı c)Destinasyon sürdürülebilirlik faaliyetlerinin
tanıtımda ortak kaynak ve eylem planı belirlenmiştir. Bu faktörlere ilişkin
olarak belediyelerin gerçekleştireceği eylemler sürdürülebilirlik
çalışmalarının turistik hizmet kalitesini ve turistik talebi olumlu
etkilemesini sağlayacaktır.
AZERBAYCAN – SOVYET YAZARLARININ YARATICILIĞINDA TÜRKİYE: 1960-1980 DÖNEMİ KAYNAKLARINA BİR BAKIŞ
Authors: AHMADOV Namiq
Published: March 2019

Sovyet lideri
Stalin’in ölümünden sonra demir  perdelerin kaldırılması Türkiye ile
ilişkilerin yeniden oluşturulmasında yeni bir dönem başlattı. Sovyetler
Birliği ile Türkiye arasında yapılan bilimsel ve kültürel anlaşmalardan, yani
1964 senesinden sonra Azerbaycan’dan birçok yazar, sanat ve bilim insanının
Türkiye’ye seyahati için olanaklar sağlandı. Azerbaycan Sovyet basınında, Türkiye ile
ilgili konularda yazılarıyla toplumun merakını gidermeye çalışan ilk isimler
arasında, Mehdi Hüseyin, Gasım Gasımzadə,
Elçin ve Memmed Aslan’ın adlarını anmamız gerekir. Azerbaycan’ın ünlü yazarı, dramaturg,
eleştirmen, aydın Mehdi Hüseyin’in Türkiye’ye ilk kez gidişi ve 1964 senesinde “Bir Ay ve Bir Gün” adlı kitabının okurlara sunulması   dönemin
en önemli olaylarından birisiydi. Daha sonraki dönemlerde  Gasım Gasımzade, Elçin ve Memmed Aslan’ın Türkiye
ile ilgili kitapları da  yayınlar
arasında yerini almıştı.Azerbaycan-Türkiye
ilişkileri  bakımından
değerlendirildiğinde,  eleştirmenler
tarafından  bugün bile  edebiyatın iyi  örnekleri olarak  kabul edilen 
bu değerli sanat eserleri bu konuda  yazılmış ilk örnekler olarak  kabul edilmektedir.

İzvestiya
gazetesinin Türkiye’deki muhabirleri olarak 
çalışan Azad Şerifov ve Zaur Gadimbeyov’un  kardeş Türkiye’de yaşadıklarıyla ilgili
izlenimlerinin yer aldığı eserleri de Sovyet döneminde ses getirmiştir.
Análise dos efeitos agudos do alongamento estático em treinamento de força: uma revisão sistemática
Authors: Brezezinski Beatriz Bazilio , Martins Flavia Angela Servat , Malfatti Carlos Ricardo Maneck , Silva Luiz Augusto , Martins Marcelo Eduardo de Almeida , Martins Julio Cesar Lacerda
Published: March 2019

Esta pesquisa teve como temática analisar os efeitos agudos do alongamento estático em treinamento de força. O problema constou do seguinte quesito: preencher lacunas que possam existir sobre o tema, buscando por meio desta pesquisa quais os efeitos deste tipo de alongamento previamente ao exercício resistido. O objetivo foi realizar uma busca referente ao tema da influência do alongamento estático ao treinamento resistido. A metodologia precedeu de uma abordagem qualitativa, com delineamento em uma revisão sistemática. Concluímos que o alongamento muscular provocou efeito deletério, prejudicando o desempenho dos avaliados.
Análisis comparativo de la vegetación de fundo legal y la vegetación madura en el poblado de Yaxcabá, Yucatán, México
Authors: Rodríguez-Sánchez Perla Victoria , Levy-Tacher Samuel Israel , Ramírez-Marcial Neptalí , Estrada-Lugo Erin
Published: March 2019

Antecedentes: El fundo legal (FL) representa una franja de vegetación que rodea a los poblados de la península de Yucatán. Su principal función es el aprovisionamiento de servicios ecosistémicos.Hipótesis: ¿La composición y estructura de la vegetación leñosa del FL difiere al compararse con vegetación madura (VM)? El FL y la VM son similares en cuanto a su riqueza florística pero no en su estructura.Lugar y fechas de estudio: En VM y vegetación del FL de la cabecera municipal de Yaxcabá, Yucatán durante los meses de febrero a julio de 2017.Métodos: Se realizaron 32 unidades de muestreo (UM) en dos tipos de suelo predominante (hollum y kankab); 24 UM se realizaron dentro del FL y 8 UM en VM. En cada UM se registraron a todos los individuos ? 2.5 cm de diámetro. La riqueza y diversidad de especies se comparó entre FL y VM mediante la rarefacción para estandarizar el esfuerzo de muestreo y a través de un modelo lineal y una prueba post hoc se definieron las diferencias estructurales entre ambos tipos de vegetación.Resultados: Los resultados revelaron similitud en la riqueza, densidad de árboles y diversidad de especies entre la vegetación de FL y VM. Sin embargo, el área basal fue significativamente menor en el FL.Conclusiones: Las altas similitudes en composición y parte de la estructura de la vegetación del FL con respecto a la VM, es una fuerte evidencia del aprovechamiento sustentable de la vegetación por los pobladores de Yaxcabá.
Morphological differences of Turnera diffusa (Turneraceae) in two populations differing in rainfall at Northeastern Mexico
Authors: Urbizu-González Ana Lucía , Flores Joel , De la Rosa-Manzano Edilia , Castillo-Ruíz Octelina
Published: March 2019

Background. Plant morphology is the expression of a set of characteristics in response to environmental conditions and therefore to the availability of resources.Questions. Are the populations of Turnera diffusa morphologically different due to climatic factors?Species studied. Turnera diffusa , known as “damiana”.Study site and dates. Jaumave and Güémez in Tamaulipas, Mexico; during October 2013 and April 2014.Methods. Abiotic characteristics of study sites and morphological traits of the plants were evaluated, as well as the exposure of the plants to light, and the flowering; 15 random transects of 100 m2 each were made in each site. A multivariate analysis with linear discriminant functions was applied to determine possible differences between the two populations considering the morphological variables.Results. The population of T. diffusa of Güémez did present higher branch density and bigger leaves than those of Jaumave, which can be related to the greater precipitation that is received in that locality in comparison with Jaumave. T. diffusa plants with higher height were found under natural shade conditions and flowering is a condition that seems to be unrelated to the exposure. Conclusions. Populations of Turnera diffusa of Jaumave and Güémez were morphologically different.
O ENSINO DA CIÊNCIA DO ETHOS EM UM PAÍS RELIGIOSO
Authors: Santos Claudecir Dos
Published: March 2019

Tendo como referência o Projeto de Lei do Senado Federal, PLS Nº 2 de 2012, e a proposição das discilinas de Cidadania Moral e Ética, e, Ética Social e Política na educação básica, como iniciativa para fazer frente ao “esvaziamento ético no Brasil”, o presente artigo problematiza essa afirmação indagando-se sobre as relações de um “esvaziamento ético” com as revelações de um moralismo hipócrita. O artigo procura mostrar que a sugestão/criação de projetos educacionais que pretendem superar a "crise de valores humanos” fundamentados na ética e na moral, mas que ignoram ou desconhecem o conteúdo semântico da ciência do ethos, correm o risco de se tonarem estéreis.
Биосфера и человек: нравственный аспект
Authors: Хорина Галина Петровна
Published: March 2019

Автор рассматривает нравственный аспект использования «коллективного интеллекта» для разработки и принятия решений по предотвращению разрушения биосферы.

Статья подготовлена на основе выступления на Всероссийской научной конференции «Моисеевские чтения» (Москва, 26 июня 2018 г.).
Образ Востока в пьесе К. Марло «Тамерлан Великий»
Authors: Крылова Елизавета Евгеньевна
Published: March 2019

В статье рассмотрено, каким предстает Восток в пьесе «Тамерлан Великий» К. Марло. Автор анализирует, как этот образ соотносится с историческим Тамерланом и современным ему Востоком, каковы взаимоотношения между европейским миром и восточным в пьесе, а также насколько правомерно включать пьесу Марло (и, в принципе, творения его современников) в популярный ныне постколониальный дискурс.

В виде доклада исследование было представлено на I Всероссийской научной конференции «Кристофер Марло и его творчество в русской и мировой культуре: междисциплинарный взгляд» (Москва, 22–23 июня 2018 г.). Конференция была проведена в рамках проекта Российского фонда фундаментальных исследований (№ 18-012-00679).
Indicadores de impacto social para evaluación de proyectos de vinculación con la colectividad
Authors: Morstadt Javier Del Cioppo , Bello Vélez Melissa Isabel
Published: March 2019

En este artículo se analizan los indicadores de impacto social utilizados para la evaluación de proyectos de vinculación con la colectividad, lo que permitió establecer una propuesta de valoración de impacto social abordando la pertinencia de los proyectos de vinculación ejecutados en poblaciones vulnerables y rurales de la zonas de planificación 5 y 8 del Ecuador, a partir de la información disponible en bases de datos estadísticas de proyectos de labor comunitaria estudiantil desarrollados por los docentes y estudiantes de la Facultad de Economía Agrícola de la Universidad Agraria del Ecuador en el año 2017. Se busca la generación de conocimiento con aplicación directa a los problemas de la sociedad o el sector productivo para el períodoestudiado. Los resultados observados permitieron establecer la pertinencia social de los proyectos ejecutados. Teniendo como resultado la obtención indicadores indispensables para evaluar el impacto social de los proyectos de vinculación con la colectividad
Turismo alternativo como estrategia de desarrollo local en el municipio de Chalan – Sucre
Authors: Aguirre Bertel Angelica María , Arroyo Oviedo Lisset Paola , Navarro Mesa Clarisa Isabel
Published: March 2019

Este capítulo propone la aplicación de una metodología para la elaboración de estrategias de desarrollo local en comunidades poco favorecidas en el contexto actual de la globalización, pero con las condiciones propicias para coadyuvar el desarrollo desde el aprovechamiento de sus recursos endógenos. Tomándose como referente metodológico los lineamientos estratégicos que se desarrollan en pro de alcanzar los objetivos propuestos desde la identificación de la vocación. Así mismo, se presentan los resultados de cada fase de la metodología aplicados al municipio de Chalán en el Departamento de Sucre, donde se plantea el turismo alternativo como una estrategia de desarrollo local a través del diseño de un producto turístico que conduciría a mejorar las condiciones socioeconómicas de esta comunidad.
Relación alma-cuerpo
Authors: García Acevedo Juan Sebastián
Published: March 2019

The present article aims to establish the responsibility of Descartes in the construction of the mind-body problematic trough the dualistic separation of soul and body in modern philosophy which reach its maximum in the skepticism of body existence; and it pretends to understand the response to idealism and the transcendental realism made by Kant in the Critic of Pure Reason as a response to this Cartesian proposal.
Los clúster como herramienta para dinamizar la competitividad
Authors: Arana Londoño Ofelia , Ballesteros Madera Ana Sofía
Published: March 2019

Los clúster son herramientas que permiten mejorar la competitividad y productividad de los diferentes sectores económico, contribuyen a la generación de empleo e ingresos, lo que ha llevado a invertir en sectores estratégicos, apoyados en el Programa de Transformación Productiva- PTP-, un programa del gobierno nacional que permite mejorar el diálogo entre los actores de la cadena productiva. Es así como el objetivo de esta investigación es identificar y analizar los clúster de las diferentes regiones del país, para lo cual se utilizó como marco de referencia el modelo de Porter. Los resultados de esta investigación corroboran la importancia de la articulación de los diferentes actores de la cadena productiva que dinamizan la competitividad en los diferentes sectores y como estrategia para acelerar el crecimiento económico del país.
Extradermal melanin transfer? Lack of macroscopic spleen melanization in old C57BL/6 mice with de-synchronized hair cycle.
Authors: Michalczyk Dominika , Popik Malgorzata , Salwinski Aleksander , Plonka Przemyslaw M
Published: March 2019

In quest of alternate, extradermal path of melanin transfer from skin to the visceral organs, we suggested that some portions of such melanin may be deposited in the spleen, which in young black C57BL/6 mice is often melanized. Here, we confirm these observation using young C57BL/6 female mice (up to 17 weeks) and show that this phenomenon cannot be observed in old animals where the hair cycle is not synchronized any more. The experiments were carried out both on spontaneous and depilation-induced hair cycle. We have checked it as a side-observation over many other experiments carried out on young and old C57BL/6 female mice (up to 2.5 years of life). The presence or absence of melanin in the spleens was checked macroscopically, and histologically by Fontana-Masson (FM) staining, and synchronization of the hair cycle - by standard histomorphometric analysis of the back skin hair follicles. In about 40% of old spleens black FM-stainable "debris" could be found under closer histological examination. This study shows that, at least in part, the phenomenon of splenic melanosis in C57BL/6 mice can be correlated with the synchronized skin melanization parallel to the hair cycle progress, and that splenic melanin undergoes gradual degradation during the mouse life.
High sequence homology between protein tyrosine acid phosphatase from boar seminal vesicles and human prostatic acid phosphatase.
Authors: Wysocki Paweł , Płucienniczak Grazyna , Strzezek Jerzy
Published: March 2019

Boar seminal vesicle protein tyrosine acid phosphatase (PTAP) and human prostatic acid phosphatase (PAP) show high affinity for protein phosphotyrosine residues. The physico-chemical and kinetic properties of the boar and human enzymes are different. The main objective of this study was to establish the nucleotide sequence of cDNA encoding boar PTAP and compare it with that of human PAP cDNA. Also, the amino-acid sequence of boar PTAP was compared with the sequence of human PAP. PTAP was isolated from boar seminal vesicle fluid and sequenced. cDNA to boar seminal vesicle RNA was synthesized, amplified by PCR, cloned in E. coli and sequenced. The obtained N-terminal amino-acid sequence of boar PTAP showed 92% identity with the N-terminal amino-acid sequence of human PAP. The determined sequence of a 354 bp nucleotide fragment (GenBank accession number: GQ184596) showed 90% identity with the corresponding sequence of human PAP. On the basis of this sequence a 118 amino acid fragment of boar PTAP was predicted. This fragment showed 89% identity with the corresponding fragment of human PAP and had a similar hydropathy profile. The compared sequences differ in terms of their isoelectric points and amino-acid composition. This may explain the differences in substrate specificity and inhibitor resistance of boar PTAP and human PAP.
The slow sarco/endoplasmic reticulum Ca2+ -ATPase declines independently of slow myosin in soleus muscle of diabetic rats.
Authors: Rácz Gábor , Szabó András , Vér Agota , Zádor Erno
Published: March 2019

The sarcoplasmic reticulum Ca(2+)-ATPase (SERCA) isoforms are normally expressed in coordination with the corresponding myosin heavy chain (MyHC) isoforms in the fibers of skeletal muscle but this coordination is often disrupted in pathological conditions. In the streptozotocin-induced diabetes of rats (stz-rats), the soleus muscle showed peripheral neuropathy and the SERCA2a level decreased in type I (slow-oxidative) fibers compared to the control muscles, whereas the expression of the corresponding slow MyHC1 did not change. No difference was found at the mRNA and protein levels of SERCA and MyHC isoforms in the whole soleus, except that the level of the SERCA2a protein specifically declined in stz-rats compared to the controls. This shows that the coordinated expression of SERCA2a and MyHC1 is disrupted at the SERCA2a protein level in the diabetic soleus. The results are in line with previous observations that regulators of the Ca-homeostasis may adapt faster to type I diabetes than the contractile elements.
Impact of roscovitine, a selective CDK inhibitor, on cancer cells: bi-functionality increases its therapeutic potential.
Authors: Wesierska-Gadek Józefa , Borza Andreea , Komina Oxana , Maurer Margarita
Published: March 2019

Increased expression and activity of proteins driving cell cycle progression as well as inactivation of endogenous inhibitors of cyclin-dependent kinases (CDKs) enhance the proliferative potential of cells. Escape of cells during malignant transformation from the proper cell cycle control rendering them independent from growth factors provides rationale for therapeutic targeting of CDKs. Exposure of rapidly growing human MCF-7 breast cancer and HeLa cervix cancer cells to roscovitine (ROSC), a selective inhibitor of CDKs, inhibits their proliferation by induction of cell cycle arrest and/or apoptosis. The outcome strongly depends on the intrinsic traits of the tumor cells, on their cell cycle status prior to the onset of treatment and also on ROSC concentration. At lower dose ROSC primarily inhibits the cell cycle-related CDKs resulting in a strong cell cycle arrest. Interestingly, ROSC arrests asynchronously growing cells at the G(2)/M transition irrespective of the status of their restriction checkpoint. However, the exposure of cancer cells synchronized after serum starvation in the late G(1) phase results in a transient G(1) arrest only in cells displaying the intact G(1)/S checkpoint. At higher dosage ROSC triggers apoptosis. In HeLa cells inhibition of the activity of CDK7 and, in consequence, that of RNA polymerase II is a major event that facilitates the initiation of caspase-dependent apoptosis. In contrast, in the caspase-3-deficient MCF-7 breast cancer cells ROSC induces apoptosis by a p53-dependent pathway. HIPK2-mediated activation of the p53 transcription factor by phosphorylation at Ser46 results in upregulation of p53AIP1 protein. This protein after de novo synthesis and translocation into the mitochondria promotes depolarization of the mitochondrial membrane.
New bradykinin B(2) receptor antagonists - influence of C-terminal segment modifications on their pharmacological properties.
Authors: Sleszyńska Małgorzata , Kwiatkowska Anna , Sobolewski Dariusz , Wierzba Tomasz H , Katarzyńska Joanna , Zabrocki Janusz , Borovickova Lenka , Slaninová Jirina , Prahl Adam
Published: March 2019

In the present study we describe the synthesis and some pharmacological properties of eight new analogues of bradykinin (BK). Two peptides were designed by substitution of position 7 or 8 of the known [D-Arg(0),Hyp(3),Thi(5,8),D-Phe(7)]BK antagonist (Stewart's antagonist) with L-pipecolic acid (L-Pip). The next two analogues were obtained by replacement of the d-Phe residue in position 7 of the Stewart's peptide with L-beta(2)-isoproline (L-beta(2)-iPro) or L-beta(3)-homoproline (L-beta(3)-hPro). The four analogues mentioned above were also prepared in N-acylated form with 1-adamantaneacetic acid (Aaa). Biological activity of the compounds was assessed by isolated rat uterus and rat blood pressure tests. Our results showed that L-Pip in position 7 slightly increased antagonistic potency in the blood pressure test, but it turned the analogue into an agonist in the rat uterus test. Replacement of Thi by L-Pip in position 8 also enhanced antagonism in the rat pressure test but preserved the antagonism in the rat uterus test. L-beta(2)-iPro or L-beta(3)-hPro in position 7 decreased the potencies in both tests. We also demonstrated that acylation of the N-terminus did not increase, as was claimed previously, the antagonistic potencies of the resulting peptides. The results thus support the hypothesis about the existence of different types of BK receptors in the rat uterus and blood vessels. Our studies provide new information about the structure-activity relationship of BK antagonists which may help in designing more potent BK receptor blockers.
Expression and activity of superoxide dismutase isoenzymes in colorectal cancer.
Authors: Skrzycki Michał , Majewska Monika , Podsiad Małgorzata , Czeczot Hanna
Published: March 2019

The aim of the study was an evaluation of changes in protein level and activity of SOD isoenzymes, and the participation of AP-1 and NF-kappaB in subsequent stages of colorectal cancer development. Studies were conducted on 65 colorectal cancers. Controls were unchanged colon regions. Activity of SOD isoenzymes, lipid peroxidation level (TBARS), and protein level of SOD1, SOD2, AP-1 and NF-kappaB were determined. We found that the protein level and activity of SOD isoenzymes and protein level of AP-1 and NF-kappaB change in subsequent stages of clinical advancement of colorectal cancer, according to UICC (I-IV), and in grades of tumor cells differentiation (G(1)-G(3)). These results indicate adaptation of colorectal cancer cells to oxidative stress, and show that the observed changes of SOD activity and protein level depend on gradual progression of colorectal cancer, and suggest an impairment of processes regulated by AP-1 and NF-kappaB which are critical for tumor progression (proliferation, differentiation and apoptosis).
Cardioprotective effect of 5-lipoxygenase gene (ALOX5) silencing in ischemia-reperfusion.
Authors: Lisovyy Oleksandr O , Dosenko Victor E , Nagibin Vasyl S , Tumanovska Lesya V , Korol Maria O , Surova Olga V , Moibenko Olexiy O
Published: March 2019

It is well known that 5-lipoxygenase derivates of arachidonic acid play an important pathogenic role during myocardial infarction. Therefore, the gene encoding arachidonate 5-lipoxygenase (ALOX5) appears to be an attractive target for RNA interference (RNAi) application. In experiments on cultivated cardiomyocytes with anoxia-reoxygenation (AR) and in vivo using rat model of heart ischemia-reperfusion (IR) we determined influence of ALOX5 silencing on myocardial cell death. ALOX5 silencing was quantified using real-time PCR, semi-quantitative PCR, and evaluation of LTC(4) concentration in cardiac tissue. A 4.7-fold decrease of ALOX5 expression (P < 0.05) was observed in isolated cardiomyocytes together with a reduced number of necrotic cardiomyocytes (P < 0.05), increased number live (P < 0.05) and unchanged number of apoptotic cells during AR of cardiomyocytes. Downregulation of ALOX5 expression in myocardial tissue by 19% (P < 0.05) resulted in a 3.8-fold reduction of infarct size in an open chest rat model of heart IR (P < 0.05). Thus, RNAi targeting of ALOX5 protects heart cells against IR injury both in culture and in vivo.
Evaluation of mixed-salt effects on thermodynamic and kinetic parameters of RNA polymerase-promoter DNA complexes in terms of equivalent salt concentrations. General applicability to DNA complexes.
Authors: Łoziński Tomasz , Wierzchowski Kazimierz L
Published: March 2019

Facile evaluation of mixed-salt effect on the strongly salt-dependent thermodynamic and kinetic parameters of protein-DNA complexes is of importance for relevant biochemical and biophysical studies. In pursuit of this aim, binding isotherms for open transcription complex (RPo) of Escherichia coli RNA polymerase (R) at lambdaP(R) promoter DNA (P) were determined as a function of salt concentration in pure NaCl and Tris/HCl solutions, and as a function of [NaCl] in the presence of fixed concentrations of MgCl(2) and Tris/HCl. A concept of equivalent salt concentrations, i.e. concentrations at which the binding equilibrium constant is the same, was introduced and applied for prediction of binding isotherms in mixed salt solutions. Full coincidence between the experimental and predicted isotherms indicated that individual contributions of salts to the global salt-effect are additive in a broad range of salt concentrations. A generalized formula for calculation of salt equivalents characteristic for any of the thermodynamic or kinetic parameters of a complex (e.g., free energy, binding equilibrium and association/dissociation kinetic rate constants) is presented and its applicability to a number of protein-DNA complexes and dsDNA melting demonstrated using authors' own and literature data.
Two Gln187 mutants of human soluble APRIL inhibit proliferation of lung carcinoma A549 cells.
Authors: Dai Shuangshuang , Zheng Yingru , Chen Bin , Gao Min , Zhang Yan , Zhang Li , Gong Wei , He Fengtain
Published: March 2019

Soluble APRIL (sAPRIL), the active form of a proliferation-inducing ligand (APRIL), is implicated in the proliferation of tumor cells. Suppressing APRIL function has been considered as a potential strategy for the therapy of APRIL-associated tumors. In the present study, we generated human sAPRIL and its two mutants, Gln187-D-sAPRIL (Gln187 deleted) and Gly187-sAPRIL (Gln187 replaced by Gly). In vitro experiments showed that the two mutants had similar specific binding capacity to lung carcinoma A549 cells compared to the wild-type sAPRIL, and both, especially Gly187-sAPRIL, exhibited significant antagonistic effect on sAPRIL-induced tumor cell proliferation in a dose-dependent manner, which might be predominantly mediated by blocking sAPRIL-induced MEK and ERK phosphorylation but not p38MAPK or JNK signaling. In vivo experiments with nude mice bearing A549 cell-derived xenograft tumor showed that only the Gly187-sAPRIL mutant could significantly suppress the tumor growth. These results suggest that Gln187 may be a crucial amino acid in APRIL-mediated tumor cell proliferation via the MEK-ERK signaling pathway and that the sAPRIL mutants may serve as novel potential antagonists of APRIL for the therapy of APRIL-associated cancers.
Regulation of cell function by isoforms of C-reactive protein: a comparative analysis.
Authors: Boncler Magdalena , Watała Cezary
Published: March 2019

Despite the emerging evidence suggesting a proatherogenic role of C-reactive protein (CRP) in atherosclerosis, the contribution of CRP in pathogenesis of atherosclerosis and atherothrombosis has not been unequivocally defined. The role of CRP in pathophysiology/pathology seems to largely depend on its structure. Two CRP isoforms, the native pentameric and the modified monomeric one, differ substantially in their physiological functions, which is thought to origin from the considerable structural heterogeneity of the CRP molecule. The present review provides an overview of the experimental evidence with relevance to the clinical role(s) of various CRP isoforms. The biological role of the protein, its structure and distribution are discussed with particular emphasis on the diverse properties of the pentameric and monomeric forms of CRP. Some methodological aspects, related to experimental models and techniques of CRP preparation, are also critically reviewed.