bluuebunny/crossref_metadata_2025 huggingface dataset:
The Effects of Private Damage Claims on Cartel Activity: Experimental Evidence
Authors: Bodnar Olivia , Fremerey Melinda , Normann Hans-Theo , Schad Jannika
Published: June 2021

Private damage claims against cartels may have negative effects on leniency: whereas whistleblowers obtain full immunity regarding the public cartel fines, they have no or only restricted protection against private third-party damage claims. This may stabilize cartels. We run an experiment to study this issue. Firms choose whether to join a cartel, may apply for leniency afterward, and then potentially face private damages. We find that the implementation of private damage claims reduces cartel formation but makes cartels indeed more stable. The negative effect of damages is avoided in a novel setting where the whistleblower is also protected from damages (JEL C90, L41, L44).
Nächstenliebe in the Age of Nationalism: Reclaiming a Jewish Emotion between the Fin-de-Siècle and the First World War
Authors: Bailey Christian
Published: June 2021

This article asks why the concept of ‘Nächstenliebe’ or ‘love of neighbour’ became a source of contestation between Jewish and Christian intellectuals between the fin-de-siècle and the First World War. For some Christian intellectuals, highlighting differences between an apparently more expansive Christian love of neighbour and an allegedly narrower Jewish conception could provide a novel means of questioning how fully Jews could belong as members of German society. As the article shows, Jewish intellectuals did not simply seek to counter those definitions that claimed a moral superiority for Christian Nächstenliebe, however. Some went further and argued that Jewish ideas of neighbourly love were actually the more cosmopolitan, in contrast to Christian conceptions that had become increasingly self-serving and sectarian. In some instances, these Jewish intellectuals used such arguments about love of neighbour to make the case for Zionism. The article then investigates how the First World War affected these ideas about Nächstenliebe. It suggests that the war furthered the ‘negative integration’ of German society in ways that left little room for love between diverse kinds of German.
Strain Counterstrain Pain Treatment: A Solution for Military Members to Succeed
Authors: Biery John C , Savoia-McHugh Kimberly S , Savoia-McHugh Lee A
Published: June 2021

Military members are required to perform in austere environments in which standard medical care routinely provided in the civilian setting is not available. Medical problems requiring hospital-based treatment which is not available in the field, shipboard, or deployed setting can be a cause for military members to be permanently discharged from active duty for medical reasons. We present a case of chronic low back pain treated with epidural steroid injections not routinely available aboard ship. The member was found unfit for shipboard duties, potentially ending her career in the military. The patient’s low back pain resolved with Strain Counterstrain (SCS) techniques. Additionally, SCS treatments also resolved undisclosed chronic pelvic pain, leading to improved overall quality of life. Strain Counterstrain is a non-interventional treatment which does not require special equipment, is available in austere environments and aboard ship, and allowed the member to remain on active duty. Strain Counterstrain is a manual muscle-retraining procedure easily learned, which can be performed in the field, on ship, in the deployed setting, in primary care, as well as in specialty pain medicine clinics.
On accretion discs formed in MHD simulations of black hole–neutron star mergers with accurate microphysics
Authors: Most Elias R , Papenfort L Jens , Tootle Samuel D , Rezzolla Luciano
Published: June 2021

Remnant accretion discs formed in compact object mergers are an important ingredient in the understanding of electromagnetic afterglows of multimessenger gravitational-wave events. Due to magnetically and neutrino-driven winds, a significant fraction of the disc mass will eventually become unbound and undergo r-process nucleosynthesis. While this process has been studied in some detail, previous studies have typically used approximate initial conditions for the accretion discs, or started from purely hydrodynamical simulations. In this work, we analyse the properties of accretion discs formed from near equal-mass black hole–neutron star mergers simulated in general-relativistic magnetohydrodynamics in dynamical spacetimes with an accurate microphysical description. The post-merger systems were evolved until $120\, {\rm ms}$ for different finite-temperature equations of state and black hole spins. We present a detailed analysis of the fluid properties and of the magnetic-field topology. In particular, we provide analytic fits of the magnetic-field strength and specific entropy as a function of the rest-mass density, which can be used for the construction of equilibrium disc models. Finally, we evolve one of the systems for a total of $350\, \rm ms$ after merger and study the prospect for eventual jet launching. While our simulations do not reach this stage, we find clear evidence of continued funnel magnetization and clearing, a prerequisite for any jet-launching mechanism.
A new photometric and dynamical study of the eclipsing binary star HW Virginis
Authors: Brown-Sevilla S B , Nascimbeni V , Borsato L , Tartaglia L , Nardiello D , Granata V , Libralato M , Damasso M , Piotto G , Pollacco D , West R G , Colombo L S , Cunial A , Piazza G , Scaggiante F
Published: June 2021

A growing number of eclipsing binary systems of the ‘HW Virginis’ (HW Vir) kind (i.e. composed by a subdwarf-B/O primary star and an M dwarf secondary) show variations in their orbital period, also called eclipse time variations (ETVs). Their physical origin is not yet known with certainty: While some ETVs have been claimed to arise from dynamical perturbations due to the presence of circumbinary planetary companions, other authors suggest that the Applegate effect or other unknown stellar mechanisms could be responsible for them. In this work, we present 28 unpublished high-precision light curves of one of the most controversial of these systems, the prototype HW Vir. We homogeneously analysed the new eclipse timings together with historical data obtained between 1983 and 2012, demonstrating that the planetary models previously claimed do not fit the new photometric data, besides being dynamically unstable. In an effort to find a new model able to fit all the available data, we developed a new approach based on a global-search genetic algorithm and eventually found two new distinct families of solutions that fit the observed timings very well, yet dynamically unstable at the 105-yr time-scale. This serves as a cautionary tale on the existence of formal solutions that apparently explain ETVs but are not physically meaningful, and on the need of carefully testing their stability. On the other hand, our data confirm the presence of an ETV on HW Vir that known stellar mechanisms are unable to explain, pushing towards further observing and modeling efforts.
Self-sustaining vortices in protoplanetary discs: Setting the stage for planetary system formation
Authors: Regály Zsolt , Kadam Kundan , Dullemond Cornelis P
Published: June 2021

The core accretion scenario of planet formation assumes that planetesimals and planetary embryos are formed during the primordial, gaseous phases of the protoplanetary disc. However, how the dust particles overcome the traditional growth barriers is not well understood. The recently proposed viscous ring-instability may explain the concentric rings observed in protoplanetary discs by assuming that the dust grains can reduce the gas conductivity, which can weaken the magnetorotational instability. We present an analysis of this model with the help of GPU-based numerical hydrodynamic simulations of coupled gas and dust in the thin-disc limit. During the evolution of the disc the dusty rings become Rossby unstable and breakup into a cascade of small-scale vortices. The vortices form secularly stable dusty structures, which could be sites of planetesimal formation by the streaming instability as well as direct gravitational collapse. The phenomenon of self-sustaining vortices is consistent with observational constraints of exoplanets and sets a favourable environment for planetary system formation.
Physical conditions of iron-peak low-ionization lines in the FeLoBAL quasar Q0059-2735
Authors: Xu Xinfeng , Arav Nahum , Miller Timothy , Korista Kirk T , Benn Chris
Published: June 2021

Quasar Q0059–2735, one of the first known iron low-ionization broad-absorption-line quasars (FeLoBAL), has a plethora of outflow absorption features at different velocities. Given multiple outflow systems, their troughs from high-ionization transitions form very wide BAL features, e.g. C iv troughs extend from ∼−1000 to −25 000 km s−1. The troughs from low-ionization transitions show more than 1000 narrow absorption lines (NALs) with velocities from −1000 to −3000 km s−1. These include troughs from iron-peak elements, e.g. Fe ii, Fe iii, Cr ii, Mn ii, Ni ii, and Co ii, which are rarely detected in quasar outflows. Most of these troughs are non-black saturated. We constrain the physical conditions of the NALs by fitting the observed Fe ii and Fe iii absorption troughs. We find that the Fe ii absorption arises from a region with an electron temperature (Te) of ∼8000 K and an electron number density (ne) of ∼108 cm−3. The same model also fits well the troughs from other iron-peak elements. In contrast to the Fe ii lines, Fe iii lines are formed in a hotter region, i.e. Te ∼20 000 K. To fit the Fe ii and Fe iii lines simultaneously in a single photoionized cloud, they require a supersolar iron abundance and/or other heating mechanisms for the Fe iii region. The distance (R) of the outflows to the central quasar is determined to be ∼40 pc. The high-resolution data afforded by the Very Large Telescope (VLT)/UVES observations from 2006 and 2018, along with the narrow lines, allow us to constrain the smallest outflow deceleration in any known quasars.
Variation of the nebular dust attenuation curve with the properties of local star-forming galaxies
Authors: Rezaee Saeed , Reddy Naveen , Shivaei Irene , Fetherolf Tara , Emami Najmeh , Khostovan A A
Published: June 2021

We use a sample of 78 340 star-forming galaxies at z ≃ 0.04–0.1 from the Sloan Digital Sky Survey (SDSS) data release (DR8) survey to calculate the average nebular dust attenuation curve and its variation with the physical properties of galaxies. Using the first four low-order Balmer emission lines (H α, H β, H γ, and H δ) detected in the composite spectrum of all galaxies in the sample, we derive a nebular attenuation curve in the range of 0.41 to $0.66\, \mu$m that has a similar shape and normalization to that of the Galactic extinction curve (Milky Way curve), the SMC curve and the nebular attenuation curve derived recently for typical star-forming galaxies at z ∼ 2. We divide the galaxies into bins of stellar mass, gas-phase metallicity, and specific star formation rate, and derive the nebular attenuation curve in each of these bins. This analysis indicates that there is very little variation in the shape of the nebular dust attenuation curve with the properties used to bin the galaxies, and suggests a near universal shape of the nebular dust attenuation curve at least among the galaxies and the range of properties considered in our sample.
Cognate restriction of transposition by piggyBac-like proteins
Authors: Beckermann Thomas M , Luo Wentian , Wilson Catherine M , Veach Ruth Ann , Wilson Matthew H
Published: June 2021

Mobile genetic elements have been harnessed for gene transfer for a wide variety of applications including generation of stable cell lines, recombinant protein production, creation of transgenic animals, and engineering cell and gene therapy products. The piggyBac transposon family includes transposase or transposase-like proteins from a variety of species including insect, bat and human. Recently, human piggyBac transposable element derived 5 (PGBD5) protein was reported to be able to transpose piggyBac transposons in human cells raising possible safety concerns for piggyBac-mediated gene transfer applications. We evaluated three piggyBac-like proteins across species including piggyBac (insect), piggyBat (bat) and PGBD5 (human) for their ability to mobilize piggyBac transposons in human cells. We observed a lack of cross-species transposition activity. piggyBac and piggyBat activity was restricted to their cognate transposons. PGBD5 was unable to mobilize piggyBac transposons based on excision, colony count and plasmid rescue analysis, and it was unable to bind piggyBac terminal repeats. Within the piggyBac family, we observed a lack of cross-species activity and found that PGBD5 was unable to bind, excise or integrate piggyBac transposons in human cells. Transposition activity appears restricted within species within the piggyBac family of mobile genetic elements.
A comparative integrated multi-omics analysis identifies CA2 as a novel target for chordoma
Authors: Meng Tong , Huang Runzhi , Jin Jiali , Gao Jianxuan , Liu Fuyan , Wei Ziheng , Xu Xiaowen , Chang Zhengyan , Lin Jun , Ta Na , Huang Zongqiang , Yin Huabin , Zhou Wang , Song Dianwen
Published: June 2021

Background

Chordoma is a rare mesenchymal malignancy, with a high recurrence rate and unclear tumorigenic mechanism. Genetic alterations, epigenetic regulators, and chromatin spatial organization play crucial roles in the initiation and progression of chordoma. In the current study, we aim to uncover the novel therapeutical targets for chordoma via using integrated multi-omics analysis.

Methods

The RNA-sequencing (RNA-seq), assay for transposable accessible chromatin by high-throughput sequencing (ATAC-seq), and Hi-C were performed between chordoma and human nucleus pulposus (HNP), along with imageological examination and clinical information. The expressions of identified targets were validated by clinical samples and their functions were further evaluated by cell and animal experiments via gene knockdown and inhibitors.

Results

The integrated multi-omics analysis revealed the important roles of bone microenvironment in chordoma tumorigenesis. By comparing the hierarchical structures, CA2 (carbonic anhydrase II) and THNSL2 (threonine synthase-like 2) were identified in the switched compartments, cell-specific boundaries, and loops. Additionally, CA2 was highly expressed in chordoma but barely found in HNP. The cell growth and migration of chordoma cells were dramatically suppressed via inhibition of CA2 either with genetic deletion or pharmaceutical treatment with Dorzolamide HCl. Furthermore, Dorzolamide HCl also regulated the bone microenvironment by blocking the osteoclast differentiation of bone marrow monocytes.

Conclusion

This study uncovers the roles of bone microenvironment in the chordoma tumorigenesis and identifies CA2 as a novel therapeutic target for chordoma. Besides, our findings suggest Dorzolamide HCl as a promising therapeutic option for chordoma.
A novel patient stratification strategy to enhance the therapeutic efficacy of dasatinib in glioblastoma
Authors: Alhalabi Obada T , Fletcher Michael N C , Hielscher Thomas , Kessler Tobias , Lokumcu Tolga , Baumgartner Ulrich , Wittmann Elena , Schlue Silja , Göttmann Mona , Rahman Shaman , Hai Ling , Hansen-Palmus Lea , Puccio Laura , Nakano Ichiro , Herold-Mende Christel , Day Bryan W , Wick Wolfgang , Sahm Felix , Phillips Emma , Goidts Violaine
Published: June 2021

Background

Glioblastoma is the most common primary malignancy of the central nervous system with a dismal prognosis. Genomic signatures classify isocitrate dehydrogenase 1 (IDH)-wildtype glioblastoma into three subtypes: proneural, mesenchymal, and classical. Dasatinib, an inhibitor of proto-oncogene kinase Src (SRC), is one of many therapeutics which, despite promising preclinical results, have failed to improve overall survival in glioblastoma patients in clinical trials. We examined whether glioblastoma subtypes differ in their response to dasatinib and could hence be evaluated for patient enrichment strategies in clinical trials.

Methods

We carried out in silico analyses on glioblastoma gene expression (TCGA) and single-cell RNA-Seq data. In addition, in vitro experiments using glioblastoma stem-like cells (GSCs) derived from primary patient tumors were performed, with complementary gene expression profiling and immunohistochemistry analysis of tumor samples.

Results

Patients with the mesenchymal subtype of glioblastoma showed higher SRC pathway activation based on gene expression profiling. Accordingly, mesenchymal GSCs were more sensitive to SRC inhibition by dasatinib compared to proneural and classical GSCs. Notably, SRC phosphorylation status did not predict response to dasatinib treatment. Furthermore, serpin peptidase inhibitor clade H member 1 (SERPINH1), a collagen-related heat-shock protein associated with cancer progression, was shown to correlate with dasatinib response and with the mesenchymal subtype.

Conclusion

This work highlights further molecular-based patient selection strategies in clinical trials and suggests the mesenchymal subtype as well as SERPINH1 to be associated with response to dasatinib. Our findings indicate that stratification based on gene expression subtyping should be considered in future dasatinib trials.
The PI3K/Akt/mTOR pathway as a preventive target in melanoma brain metastasis
Authors: Tehranian Cedric , Fankhauser Laura , Harter Patrick N , Ratcliffe Colin D H , Zeiner Pia S , Messmer Julia M , Hoffmann Dirk C , Frey Katharina , Westphal Dana , Ronellenfitsch Michael W , Sahai Erik , Wick Wolfgang , Karreman Matthia A , Winkler Frank
Published: June 2021

Background

Brain metastases (BM) are a frequent complication of malignant melanoma (MM), with limited treatment options and poor survival. Prevention of BM could be more effective and better tolerated than treating established BM in various conditions.

Methods

To investigate the temporospatial dynamics of PI3K/Akt/mTOR (PAM) pathway activation during BM formation and the preventive potential of its inhibition, in vivo molecular imaging with an Akt biosensor was performed, and long-term intravital multiphoton microscopy through a chronic cranial window in mice.

Results

In vivo molecular imaging revealed invariable PAM pathway activation during the earliest steps of brain colonization. In order to perform a long-term intravascular arrest and to extravasate, circulating MM cells needed to activate their PAM pathway during this process. However, the PAM pathway was quite heterogeneously activated in established human brain metastases, and its inhibition with the brain-penetrant PAM inhibitor GNE-317 resulted in only modest therapeutic effects in mice. In contrast, giving GNE-317 in preventive schedules that included very low doses effectively reduced the growth rate and number of BM in two MM mouse models over time, and led to an overall survival benefit. Longitudinal intravital multiphoton microscopy found that the first, rate-limiting steps of BM formation—permanent intravascular arrest, extravasation, and initial perivascular growth—are most vulnerable to dual PI3K/mTOR inhibition.

Conclusion

These findings establish a key role of PAM pathway activation for critical steps of early metastatic brain colonization and reveal its pharmacological inhibition as a potent avenue to prevent the formation of clinically relevant BM.
Recurrent Tuberculosis Disease in Singapore
Authors: Gan Suay Hong , KhinMar Kyi Win , Ang Li Wei , Lim Leo K Y , Sng Li Hwei , Wang Yee Tang , Chee Cynthia B E
Published: June 2021

Background

Previously treated (ie, recurrent) tuberculosis (TB) cases account for approximately 7%–8% of incident TB globally and in Singapore. Molecular fingerprinting has enabled the differentiation of these patients into relapsed or reinfection cases.

Methods

Patient demographics, disease characteristics, and treatment information were obtained from the national TB notification registry and TB Control Unit. We performed a retrospective, case-control study to evaluate factors associated with recurrent TB disease in Singapore citizens and permanent residents with culture-positive TB from 2006 to 2013 and who developed a second episode of culture-positive TB up to 2016 using multivariable logistic regression analyses.

Results

Ninety-one cases with culture-positive first and recurrent TB disease episodes were identified. Recurrent TB was associated with age ≥60 years (adjusted odds ratio [aOR], 1.98 [95% confidence interval {CI}, 1.09–3.61), male sex (aOR, 2.29 [95% CI, 1.22–4.51]), having concomitant pulmonary and extrapulmonary TB (aOR, 3.10 [95% CI, 1.59–6.10]) and extrapulmonary TB alone (aOR, 3.82 [95% CI, 1.12–13.31]), and was less likely in non-Malays (aOR, 0.52 [95% CI, .27–.99]). DNA fingerprinting results for both episodes in 49 cases differentiated these into 28 relapsed and 21 reinfection cases. Relapse was associated with having concomitant pulmonary and extrapulmonary TB (aOR, 9.24 [95% CI, 2.50–42.42]) and positive sputum acid-fast bacilli smear (aOR, 3.95 [95% CI, 1.36–13.10]).

Conclusions

Relapse and reinfection contributed to 57% and 43%, respectively, of recurrent TB in Singapore. Our study highlights the underappreciated association of concomitant pulmonary and extrapulmonary TB as a significant risk factor for disease relapse.
Robotic Applications in Cranial Neurosurgery: Current and Future
Authors: Ball Tyler , González-Martínez Jorge , Zemmar Ajmal , Sweid Ahmad , Chandra Sarat , VanSickle David , Neimat Joseph S , Jabbour Pascal , Wu Chengyuan
Published: June 2021

Robotics applied to cranial surgery is a fast-moving and fascinating field, which is transforming the practice of neurosurgery. With exponential increases in computing power, improvements in connectivity, artificial intelligence, and enhanced precision of accessing target structures, robots are likely to be incorporated into more areas of neurosurgery in the future—making procedures safer and more efficient. Overall, improved efficiency can offset upfront costs and potentially prove cost-effective. In this narrative review, we aim to translate a broad clinical experience into practical information for the incorporation of robotics into neurosurgical practice. We begin with procedures where robotics take the role of a stereotactic frame and guide instruments along a linear trajectory. Next, we discuss robotics in endoscopic surgery, where the robot functions similar to a surgical assistant by holding the endoscope and providing retraction, supplemental lighting, and correlation of the surgical field with navigation. Then, we look at early experience with endovascular robots, where robots carry out tasks of the primary surgeon while the surgeon directs these movements remotely. We briefly discuss a novel microsurgical robot that can perform many of the critical operative steps (with potential for fine motor augmentation) remotely. Finally, we highlight 2 innovative technologies that allow instruments to take nonlinear, predetermined paths to an intracranial destination and allow magnetic control of instruments for real-time adjustment of trajectories. We believe that robots will play an increasingly important role in the future of neurosurgery and aim to cover some of the aspects that this field holds for neurosurgical innovation.
Characterization of Nannochloropsis oceanica Rose Bengal Mutants Sheds Light on Acclimation Mechanisms to High Light When Grown in Low Temperature
Authors: Ben-Sheleg Avraham , Khozin-Godberg Inna , Yaakov Beery , Vonshak Avigad
Published: June 2021

A barrier to realizing Nannochloropsis oceanica’s potential for omega-3 eicosapentaenoic acid (EPA) production is the disparity between conditions that are optimal for growth and those that are optimal for EPA biomass content. A case in point is temperature: higher content of polyunsaturated fatty acid, and especially EPA, is observed in low-temperature (LT) environments, where growth rates are often inhibited. We hypothesized that mutant strains of N. oceanica resistant to the singlet-oxygen photosensitizer Rose Bengal (RB) would withstand the oxidative stress conditions that prevail in the combined stressful environment of high light (HL; 250 μmol photons m−2 s−1) and LT (18°C). This growth environment caused the wild-type (WT) strain to experience a spike in lipid peroxidation and an inability to proliferate, whereas growth and homeostatic reactive oxygen species levels were observed in the mutant strains. We suggest that the mutant strains’ success in this environment can be attributed to their truncated photosystem II antennas and their increased ability to diffuse energy in those antennas as heat (non-photosynthetic quenching). As a result, the mutant strains produced upward of four times more EPA than the WT strain in this HL–LT environment. The major plastidial lipid monogalactosyldiacylglycerol was a likely target for oxidative damage, contributing to the photosynthetic inhibition of the WT strain. A mutation in the NO10G01010.1 gene, causing a subunit of the 2-oxoisovalerate dehydrogenase E1 protein to become non-functional, was determined to be the likely source of tolerance in the RB113 mutant strain.
Comparison of Remote Electrical Neuromodulation and Standard-Care Medications for Acute Treatment of Migraine in Adolescents: A Post Hoc Analysis
Authors: Hershey Andrew D , Irwin Samantha , Rabany Liron , Gruper Yaron , Ironi Alon , Harris Dagan , Sharon Roni , McVige Jennifer
Published: June 2021

Objective

There is an unmet need for new, efficacious, well-tolerated, acute treatments for migraine in adolescents. Remote electrical neuromodulation (REN) is a novel, nonpharmacological treatment that provides significant symptom relief with good tolerability. The current post hoc analysis compared the efficacy of REN to that of standard-care medications for the acute treatment of migraine in adolescents.

Design

Within-participant post hoc analysis of data from a clinical trial.

Setting

Data from a clinical trial.

Subjects

Data from 35 adolescent participants were analyzed.

Methods

Efficacy was compared between a run-in phase, in which attacks were treated with standard-care medications (triptans or over-the-counter medications), and an intervention phase, in which attacks were treated with REN. Efficacy was compared within participants through the use of McNemar’s test at four endpoints (2 hours after treatment): single-treatment pain freedom and pain relief, and consistency of pain freedom and pain relief (defined as response in at least 50% of the available first four treatments).

Results

At 2 hours after treatment, pain freedom was achieved by 37.1% of the participants with REN, vs 8.6% of the participants with medications (P = 0.004). Pain relief was achieved by 71.4% with REN, vs 57.1% with medications (P = 0.225). Consistency of pain freedom was achieved by 40% with REN, vs 8.6% with medications (P < 0.001). Consistency of pain relief was achieved by 80.0% with REN, vs 57.2% with medications (P = 0.033).

Conclusions

Our results suggest that REN may have higher efficacy than certain standard-care medications for the acute treatment of migraine in adolescents. A larger-scale, blinded comparative-effectiveness and tolerability study is needed.
Effectiveness of High-Frequency Transcranial Magnetic Stimulation and Physical Exercise in Women With Fibromyalgia: A Randomized Controlled Trial
Authors: Izquierdo-Alventosa Ruth , Inglés Marta , Cortés-Amador Sara , Gimeno-Mallench Lucia , Sempere-Rubio Núria , Serra-Añó Pilar
Published: June 2021

Objective

Fibromyalgia (FM) is characterized by chronic widespread pain and both physical and emotional alterations, which in turn may affect the individual’s quality of life. Thus, interventions aimed at treating such symptoms, without increasing fatigue, are needed. The aim of this study was to explore the effect of high-frequency transcranial magnetic stimulation (HF-TMS) and physical exercise (PE) on pain, impact of FM, physical conditioning, and emotional status in women with FM.

Methods

Forty-nine women with FM were randomly allocated to: (1) a PE group (PEG, n = 16), who underwent an 8-week (two 60-minute sessions/wk) low-intensity PE program; (2) a TMS group (TMSG, n = 17) receiving a 2-week (five 20-minute sessions/wk) HF-TMS intervention; and (3) a control group (CG, n = 16). Pain (ie, perceived pain and average pressure pain threshold), perceived impact of FM (ie, overall impact, symptoms, and perceived physical function), physical conditioning (ie, endurance and functional capacity, fatigue, gait velocity, and power), and emotional status (ie, anxiety, depression, stress, and satisfaction) were assessed at baseline (T0) and after the intervention (T1, at 2 weeks for TMSG and at 8 weeks for PEG and CG).

Results

The TMSG showed significant improvement in all studied variables after the intervention except for satisfaction, whereas the PEG showed improved average pressure pain threshold, perceived overall impact of FM and total score, endurance and functional capacity, velocity and power, anxiety, depression, and stress. In contrast, the CG showed no improvements in any variable.

Conclusion

Both PE and HF-TMS are effective in improving pain, impact of FM, physical conditioning, and emotional status in people with FM; HF-TMS achieved larger improvements in emotional status than PE.

Impact

TMS and PE have similar benefits for physical status, whereas TMS has greater benefits than PE for emotional status in women with FM.
Biologic use in psoriatic arthritis and ankylosing spondylitis patients: a descriptive epidemiological study using linked, routine data in Wales, UK
Authors: Cooksey Roxanne , Rahman Muhammad Azizur , Kennedy Jonathan , Brophy Sinead , Choy Ernest
Published: June 2021

Objectives

PsA and AS are chronic diseases associated with significant morbidities. National and international management guidelines include treatment with biologic therapies to improve outcomes and quality of life. There are limited real-world data on the patients’ journey from symptom onset to diagnosis and treatment in the UK. We use real-life, linked health data to explore patient pathways and the impact of biologics on patient outcomes.

Methods

Data from the Secure Anonymised Information Linkage databank in Wales were used to assess diagnosis and treatment of patients ≥18 years of age with at least one International Classification of Diseases, Tenth Revision code present for PsA/AS in rheumatology clinic data and at least one Read code present in primary care records. We investigated the use of biologics while exploring demographics, comorbidities and surgical procedures of 641 AS patients and 1312 PsA patients.

Results

AS patients were significantly younger at diagnosis and were predominantly male. The average time from presenting symptoms to diagnosis of AS and PsA was 7.9 (s.d. 5.5) and 9.3 (s.d. 5.5) years, respectively. The proportion of patients receiving biologic treatment was significantly higher in AS (46%) compared with PsA patients (28.8%); of these, 23.1% of AS and 22.2% of PsA patients stopped/switched a biologic. There was a significant reduction in primary care involvement, sick notes and disability living allowance for both AS and PsA patients following biologic initiation.

Conclusion

This real-world descriptive study confirms that patients treated with biologics have reduced disability and time off work despite being initiated ∼13 years after the first symptoms and 6 years after diagnosis.
Evaluating Species Delimitation Methods in Radiations: The Land SnailAlbinaria cretensisComplex on Crete
Authors: Bamberger Sonja , Xu Jie , Hausdorf Bernhard
Published: June 2021

Delimiting species in radiations is notoriously difficult because of the small differences between the incipient species, the star-like tree with short branches between species, incomplete lineage sorting, and the possibility of introgression between several of the incipient species. Next-generation sequencing data may help to overcome some of these problems. We evaluated methods for species delimitation based on genome-wide markers in a land snail radiation on Crete. Species delimitation in the Albinaria cretensis group was based exclusively on shell characters until now and resulted in classifications distinguishing 3–9 species. We generated sequences of 4270 loci for 140 specimens of the A. cretensis group from 48 populations by double-digest restriction site-associated DNA sequencing. We evaluated three methods for species discovery. The multispecies coalescent approach implemented in the program Bayesian Phylogenetics and Phylogeography resulted in a drastic overestimating of the number of species, whereas Gaussian clustering resulted in an overlumping. Primary species hypotheses based on the maximum percentage of the genome of the individuals derived from ancestral populations as estimated with the program ADMIXTURE moderately overestimated the number of species, but this was the only approach that provided information about gene flow between groups. Two of the methods for species validation that we applied, BFD* and delimitR, resulted in an acceptance of almost all primary species hypotheses, even such based on arbitrary subdivisions of hypotheses based on ADMIXTURE. In contrast, secondary species hypotheses, resulting from an evaluation of primary species hypotheses based on ADMIXTURE with isolation by distance tests, approached the morphological classification, but also uncovered two cryptic species and indicated that some of the previously delimited units should be combined. Thus, we recommend this combination of approaches that provided more detailed insights in the distinctness of barriers between the taxa of a species complex and the spatial distribution of admixture between them than the other methods. The recognition and delimitation of undersampled species remained a major challenge. [ADMIXTURE; Clausiliidae; delimitR, Gaussian clustering; isolation-by-distance; land snails; multispecies coalescent; species delimitation.]
Effects of dietary chromium propionate and space allowance on performance and carcass responses of growing-finishing pigs
Authors: Santos Alexandre P , Tokach Mike D , Kiefer Charles , Goodband Robert D , Woodworth Jason C , DeRouchey Joel M , Dritz Steve S , Gebhardt Jordan T
Published: June 2021

In a 72-d trial, 256 pigs (Line 600 × 241, DNA Columbus, NE) were used to determine the effect of dietary Cr (Cr propionate; Kemin Industries, Des Moines, IA) and physical space restriction on performance and carcass characteristics of finishing pigs. Pens were blocked by initial weight and randomly assigned to treatments with eight pigs per pen and eight pens per treatment. Treatments were arranged in a 2 × 2 factorial with main effects of Cr (control and Cr propionate, 200 µg/kg added Cr) and space allowances (0.91 m2/animal: normal and 0.63 m2/animal: restricted). Pigs were fed in three dietary phases and pigs were weighed approximately every 14 d throughout the study. Feed efficiency was calculated as both a standard gain to feed ratio and as an adjusted G:F ratio at a common final bodyweight. There were no evidence of space allocation × Cr interactions for any measured responses (P > 0.05). Space restriction decreased (P < 0.001) daily weight gain, final body weight, hot carcass weight, and daily feed intake, but increased carcass yield (P = 0.009) and decreased backfat depth (P = 0.003). Feed efficiency was greater for pigs provided a normal space allowance when adjusted for a common final bodyweight (P = 0.021), although no evidence of a difference was observed for unadjusted G:F (P = 0.687). Adding Cr to the diet reduced G:F on both an adjusted and unadjusted basis (P ≤ 0.021). There was marginally significant evidence that pigs provided Cr had lower average daily gain (P = 0.079) and final bodyweight (P = 0.056) compared to pigs not provided added Cr. There was marginally significant evidence that Cr resulted in greater backfat depth (P = 0.069), although no evidence of a difference in other carcass parameters were observed (P > 0.10). These results demonstrated that there were no interactions between Cr propionate and space allocation, illustrating that under the conditions of this study Cr propionate did not provide an advantage in growth performance or carcass characteristics in either adequate or restricted space allocation.
HCV/HIV coinfected Egyptian patients: a cross-sectional study of their main characteristics and barriers to HCV treatment initiation
Authors: Eletreby Rasha , Esmat Gamal , Elsharkawy Aisha , Alsehemy Lamiaa , Mohamed Rahma , Alem Shereen Abdel , Yousof Hanaa , Cordie Ahmed , Lithy Rania M
Published: June 2021

Background

This study investigates different barriers preventing a cohort of Egyptian HIV/HCV coinfected patients from accessing HCV treatment, despite being available and free of charge, aiming to improve the long-term outcomes of coinfected patients and decreasing their liver-related morbidity and mortality.

Methods

This study included HIV patients who were referred to Kasr Alainy Viral Hepatitis Center to receive HCV treatment and who had to continue pretreatment assessment in order to receive direct acting antiviral agents free of charge. Patients who did not attend within 90 d were questioned via a telephone interview. Questions addressed sociodemographic status, HIV status and the main barriers to accessing healthcare.

Results

Overall, 474 HIV/HCV coinfected patients were eligible for HCV treatment and 223 (47.1%) patients did not complete work-up for HCV treatment. Fear of community stigma concerning HIV/HCV was the most important barrier to compliance with treatment (73.3%), followed by lack of a supportive work environment and employment opportunities (51.5%), whereas 39.3% stopped follow-up due to the lack of integrated services in the healthcare facility.

Conclusions

Managing HCV in HCV/HIV coinfected patients still represents a major challenge, not only for healthcare providers, but also at a community level, to improve community awareness and manage the major obstacle facing those patients regarding community stigma.
Automatic identification of bird females using egg phenotype
Authors: Šulc Michal , Hughes Anna E , Troscianko Jolyon , Štětková Gabriela , Procházka Petr , Požgayová Milica , Piálek Lubomír , Piálková Radka , Brlík Vojtěch , Honza Marcel
Published: June 2021

Individual identification is crucial for studying animal ecology and evolution. In birds this is often achieved by capturing and tagging. However, these methods are insufficient for identifying individuals/species that are secretive or difficult to catch. Here, we employ an automatic analytical approach to predict the identity of bird females based on the appearance of their eggs, using the common cuckoo (Cuculus canorus) as a model species. We analysed 192 cuckoo eggs using digital photography and spectrometry. Cuckoo females were identified from genetic sampling of nestlings, allowing us to determine the accuracy of automatic (unsupervised and supervised) and human assignment. Finally, we used a novel analytical approach to identify eggs that were not genetically analysed. Our results show that individual cuckoo females lay eggs with a relatively constant appearance and that eggs laid by more genetically distant females differ more in colour. Unsupervised clustering had similar cluster accuracy to experienced human observers, but supervised methods were able to outperform humans. Our novel method reliably assigned a relatively high number of eggs without genetic data to their mothers. Therefore, this is a cost-effective and minimally invasive method for increasing sample sizes, which may facilitate research on brood parasites and other avian species.
Understanding Skin Failure: A Scoping Review
Authors: Dalgleish Lizanne , Campbell Jill , Finlayson Kathleen , Barakat-Johnson Michelle , Beath Amy , Ingleman Jessica , Parker Christina , Coyer Fiona
Published: June 2021

OBJECTIVE

To map the use of the term “skin failure” in the literature over time and enhance understanding of this term as it is used in clinical practice.

DATA SOURCES

The databases searched for published literature included PubMed, Embase, the Cumulative Index for Nursing and Allied Health Literature, and Google Scholar. The search for unpublished literature encompassed two databases, Open Gray and ProQuest Dissertation and Theses.

STUDY SELECTION

Search terms included “skin failure,” “acute skin failure,” “chronic skin failure,” and “end stage skin.” All qualitative and quantitative research designs, editorial, opinion pieces, and case studies were included, as well as relevant gray literature.

DATA EXTRACTION

Data collected included author, title, year of publication, journal name, whether the term “skin failure” was mentioned in the publication and/or in conjunction with other skin injury, study design, study setting, study population, sample size, main focus of the publication, what causes skin failure, skin failure definition, primary study aim, and primary outcome.

DATA SYNTHESIS

Two main themes of skin failure were identified through this scoping review: the etiology of skin failure and the interchangeable use of definitions.

CONCLUSIONS

Use of the term “skin failure” has increased significantly over the past 30 years. However, there remains a significant lack of empirical evidence related to skin failure across all healthcare settings. The lack of quality research has resulted in multiple lines of thinking on the cause of skin failure, as well as divergent definitions of the concept. These results illustrate substantial gaps in the current literature and an urgent need to develop a globally agreed-upon definition of skin failure, as well as a better understanding of skin failure etiology.
Surgical Wound Complications after Colorectal Surgery with Single-Use Negative-Pressure Wound Therapy Versus Surgical Dressing over Closed Incisions: A Randomized Controlled Trial
Authors: León Arellano Miguel , Barragán Serrano Cristina , Guedea Manuela , Garcia Pérez Juan Carlos , Sanz Ortega Gonzalo , Guevara-Martinez Jenny , Gomez Abril Segundo , González Puga Cristina , Arroyo Antonio , Cantero Cid Ramón
Published: June 2021

BACKGROUND

Global studies indicate that surgical site infections (SSIs) are a major healthcare challenge within hospitals and can have a profound impact on patient quality of life and healthcare costs. Closed-incision negative-pressure therapy (ciNPT) has been reported to provide positive clinical benefits for patients with various incisions, including those following colorectal surgeries.

METHODS

Investigators performed a prospective, randomized, multicenter trial to evaluate complications of surgical incisions in patients who received a ciNPT dressing versus a conventional surgical dressing (control) over their closed incision following colorectal surgery. The incidence of SSI was determined at 7, 15, and 30 days postsurgery.

RESULTS

A total of 148 patients participated in the study. Results showed that the SSI rate on day 7 was lower in the ciNPT group versus the control group (10/75 [13.3%] vs 17/73 [23.3%]), but this difference was not statistically significant. On day 15, the SSI rate was 12/75 (16.0%) in the ciNPT group versus 21/73 (28.8%) in the control group; however, this difference was only marginally statistically significant (P = .0621). At 1 month, the SSI rate remained lower in the ciNPT group (13/75 [17.3%] vs 21/73 [28.8%], P = .0983) compared with the control group.

CONCLUSIONS

Future studies with larger population sizes are necessary to determine the impact of ciNPT on patients’ incisions after colorectal surgery.
Epidural Anesthesia–Analgesia and Recurrence-free Survival after Lung Cancer Surgery: A Randomized Trial
Authors: Xu Zhen-Zhen , Li Huai-Jin , Li Mu-Han , Huang Si-Ming , Li Xue , Liu Qing-Hao , Li Jian , Li Xue-Ying , Wang Dong-Xin , Sessler Daniel I.
Published: June 2021

Background

Regional anesthesia and analgesia reduce the stress response to surgery and decrease the need for volatile anesthesia and opioids, thereby preserving cancer-specific immune defenses. This study therefore tested the primary hypothesis that combining epidural anesthesia–analgesia with general anesthesia improves recurrence-free survival after lung cancer surgery.

Methods

Adults scheduled for video-assisted thoracoscopic lung cancer resections were randomized 1:1 to general anesthesia and intravenous opioid analgesia or combined epidural–general anesthesia and epidural analgesia. The primary outcome was recurrence-free survival (time from surgery to the earliest date of recurrence/metastasis or all-cause death). Secondary outcomes included overall survival (time from surgery to all-cause death) and cancer-specific survival (time from surgery to cancer-specific death). Long-term outcome assessors were blinded to treatment.

Results

Between May 2015 and November 2017, 400 patients were enrolled and randomized to general anesthesia alone (n = 200) or combined epidural–general anesthesia (n = 200). All were included in the analysis. The median follow-up duration was 32 months (interquartile range, 24 to 48). Recurrence-free survival was similar in each group, with 54 events (27%) with general anesthesia alone versus 48 events (24%) with combined epidural–general anesthesia (adjusted hazard ratio, 0.90; 95% CI, 0.60 to 1.35; P = 0.608). Overall survival was also similar with 25 events (13%) versus 31 (16%; adjusted hazard ratio, 1.12; 95% CI, 0.64 to 1.96; P = 0.697). There was also no significant difference in cancer-specific survival with 24 events (12%) versus 29 (15%; adjusted hazard ratio, 1.08; 95% CI, 0.61 to 1.91; P = 0.802). Patients assigned to combined epidural–general had more intraoperative hypotension: 94 patients (47%) versus 121 (61%; relative risk, 1.29; 95% CI, 1.07 to 1.55; P = 0.007).

Conclusions

Epidural anesthesia–analgesia for major lung cancer surgery did not improve recurrence-free, overall, or cancer-specific survival compared with general anesthesia alone, although the CI included both substantial benefit and harm.

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The impact of the COVID-19 pandemic on the field of pediatric rheumatology
Authors: Wahezi Dawn M. , Peskin Malki , Tanner Tamara
Published: June 2021

Purpose of review

The purpose of this review is to discuss the clinical management of children with pediatric rheumatic disease (PRD) during the Coronavirus disease of 2019 (COVID-19) pandemic, as well as the unique role of the pediatric rheumatologist during a time of emerging post-COVID inflammatory sequelae including, multisystem inflammatory syndrome in children (MIS-C).

Recent findings

To date, there has been little evidence to suggest that children with PRD, including those on immunomodulatory therapies, are at increased risk for severe COVID-19. Clinical guidance statements have been created to support clinical providers in providing care to children with PRD during the COVID-19 pandemic. Pediatric rheumatologists have also been called upon to assist in the identification and management of post-COVID sequelae, including the rapidly emerging inflammatory illness, MIS-C.

The COVID-19 era has been defined by a rapid expansion in scientific knowledge and a time of extraordinary local and worldwide collaboration, both within the pediatric rheumatology community, as well as across multiple disciplines. Through collective efforts, we have learned that children with PRD, including those on immunomodulatory therapies, are not at increased risk for severe COVID-19. Pediatric rheumatologists have also worked alongside other disciplines to develop guidance for the management of MIS-C, with the majority of patients experiencing excellent clinical outcomes.