bluuebunny/crossref_metadata_2025 huggingface dataset:
Selectivity Estimation of Inequality Joins in Databases
Authors: Repas Diogo , Luo Zhicheng , Schoemans Maxime , Sakr Mahmoud
Published: March 2023

Selectivity estimation refers to the ability of the SQL query optimizer to estimate the size of the results of a predicate in the query. It is the main calculation based on which the optimizer can select the least expensive plan to execute. While the problem has been known since the mid-1970s, we were surprised that there are no solutions in the literature for the selectivity estimation of inequality joins. By testing four common database systems: Oracle, SQL-Server, PostgreSQL, and MySQL, we found that the open-source systems PostgreSQL and MySQL lack this estimation. Oracle and SQL-Server make fairly accurate estimations, yet their algorithms are secret. This paper, thus, proposes an algorithm for inequality join selectivity estimation. The proposed algorithm was implemented in PostgreSQL and sent as a patch to be included in the next releases. We compared this implementation with the above DBMS for three different data distributions (uniform, normal, and Zipfian) and showed that our algorithm provides extremely accurate estimations (below 0.1% average error), outperforming the other systems by an order of magnitude.
Magnetical Manipulation of Hyperbolic Phonon Polaritons in Twisted Double-Layers of Molybdenum Trioxide
Authors: Li Hongjing , Zheng Gaige
Published: March 2023

Controlling the twist angle between double stacked van der Waals (vdW) crystals holds great promise for nanoscale light compression and manipulation in the mid-infrared (MIR) range. A lithography-free geometry has been proposed to mediate the coupling of phonon polaritons (PhPs) in double-layers of vdW α-MoO3. The anisotropic hyperbolic phonon polaritons (AHPhPs) are further hybridized by the anisotropic substrate environment of magneto-optic indium arsenide (InAs). The AHPhPs can be tuned by twisting the angle between the optical axes of the two separated layers and realize a topological transition from open to closed dispersion contours. Moreover, in the presence of external magnetic field, an alteration of the hybridization of PhPs will be met, which enable an efficient way for the control of light-matter interaction at nanoscale in the MIR region.
Microbial Composition on Abandoned and Reclaimed Mining Sites in the Komi Republic (North Russia)
Authors: Zverev Aleksei O. , Gladkov Grigory V. , Kimeklis Anastasiia K. , Kichko Arina A. , Andronov Evgeny E. , Abakumov Evgeny V.
Published: March 2023

Restoration of anthropogenically disturbed soils is an urgent problem in modern ecology and soil biology. Restoration processes in northern environments are especially important, due to the small amounts of fertile land and low levels of natural succession. We analyzed the soil microbiota, which is one of the indicators of the succession process is the soil. Samples were obtained from three disturbed soils (self-overgrown and reclaimed quarries), and two undisturbed soils (primary and secondary forests). Primary Forest soil had a well-developed soil profile, and a low pH and TOC (total organic carbon) amount. The microbial community of this soil had low richness, formed a clear remote cluster in the beta-diversity analysis, and showed an overrepresentation of Geobacter (Desulfobacteriota). Soil formation in clay and limestone abandoned quarries was at the initial stage, and was caused by both a low rate of mineral profile formation and severe climatic conditions in the region. Microbial communities of these soils did not have specific abundant taxa, and included a high amount of sparse taxa. Differences in taxa composition were correlated with abiotic factors (ammonium concentration), which, in turn, can be explained by the parent rock properties. Limestone quarry reclaimed by topsoil coverage resulted in an adaptation of the top soil microbiota to a novel parent rock. According to the CCA analysis, the microbial composition of samples was connected with pH, TOC and ammonium nitrogen concentration. Changes in pH and TOC were connected with ASVs from Chloroflexota, Gemmatimonadota and Patescibacteria. ASVs from Gemmatimonadota also were correlated with a high ammonium concentration.
Use of Shotgun Metagenomics to Assess the Microbial Diversity and Hydrocarbons Degrading Functions of Auto-Mechanic Workshops Soils Polluted with Gasoline and Diesel Fuel
Authors: Goma-Tchimbakala Emerance Jessica Claire D’Assise , Pietrini Ilaria , Goma-Tchimbakala Joseph , Corgnati Stefano Paolo
Published: March 2023

Bioaugmentation is a valuable technique for oil recovery. This study investigates the composition and functions of microbial communities in gasoline- and diesel-contaminated soils of garages Matoko (SGM) and Guy et Paul (SGP) originating from auto mechanic workshops as well as the concentration of soil enzymes β-glucosidase, β-glucosaminidase, and acid phosphatase. The work aimed to evaluate the presence of petroleum-hydrocarbon-degrading bacteria for the development of foreseen bioremediation of oil-contaminated soils. Microbial diversity, as given by shotgun metagenomics, indicated the presence of 16 classes, among which Actinobacteria and Gammaproteobacteria dominated, as well as more than 50 families, including the dominant Gordoniaceae (26.63%) in SGM and Pseudomonadaceae (57.89%) in SGP. The dominant bacterial genera in the two soils were, respectively, Gordonia (26.7%) and Pseudomonas (57.9%). The exploration of the bacterial metabolic abilities using HUMANn2 allowed to detect genes and pathways involved in alkanes and aromatic hydrocarbons in the two contaminated soils. Furthermore, enzymes β-glucosidase, β-glucosaminidase, and acid phosphatase were found in high concentrations ranging between 90.27 ± 5.3 and 804.17 ± 20.5 µg pN/g soil/h, which indicated active microbial metabolism. The high diversity of microorganisms with a hydrocarbon degradation genetic package revealed that the bacteria inhabiting the two soils are likely good candidates for the bioaugmentation of oil-contaminated soils.
Prevalence of Small Intestinal Bacterial Overgrowth Syndrome in Patients with Non-Alcoholic Fatty Liver Disease/Non-Alcoholic Steatohepatitis: A Cross-Sectional Study
Authors: Gkolfakis Paraskevas , Tziatzios Georgios , Leite Gabriela , Papanikolaou Ioannis S. , Xirouchakis Elias , Panayiotides Ioannis G. , Karageorgos Athanasios , Millan Maria Jesus , Mathur Ruchi , Weitsman Stacy , Dimitriadis George D. , Giamarellos-Bourboulis Evangelos J. , Pimentel Mark , Triantafyllou Konstantinos
Published: March 2023

Introduction: Non-alcoholic fatty liver disease (NAFLD) is a multifactorial, wide-spectrum liver disorder. Small intestinal bacterial overgrowth (SIBO) is characterized by an increase in the number and/or type of colonic bacteria in the upper gastrointestinal tract. SIBO, through energy salvage and induction of inflammation, may be a pathophysiological factor for NAFLD development and progression. Aim/Methods: Consecutive patients with histological, biochemical, or radiological diagnosis of any stage of NAFLD (non-alcoholic fatty liver [NAFL], non-alcoholic steatohepatitis [NASH], cirrhosis) underwent upper gastrointestinal endoscopy. Duodenal fluid (2cc) was aspirated from the 3rd–4th part of duodenum into sterile containers. SIBO was defined as ≥103 aerobic colony-forming units (CFU)/mL of duodenal aspirate and/or the presence of colonic-type bacteria. Patients without any liver disease undergoing gastroscopy due to gastroesophageal reflux disease (GERD) comprised the healthy control (HC) group. Concentrations (pg/mL) of tumor necrosis factor alpha (TNFα), interleukin (IL)-1β, and IL-6 were also measured in the duodenal fluid. The primary endpoint was to evaluate the prevalence of SIBO in NAFLD patients, while the comparison of SIBO prevalence among NAFLD patients and healthy controls was a secondary endpoint. Results: We enrolled 125 patients (51 NAFL, 27 NASH, 17 cirrhosis, and 30 HC) aged 54 ± 11.9 years and with a weight of 88.3 ± 19.6 kg (NAFLD vs. HC 90.7 ± 19.1 vs. 80.8 ± 19.6 kg, p = 0.02). Overall, SIBO was diagnosed in 23/125 (18.4%) patients, with Gram-negative bacteria being the predominant species (19/23; 82.6%). SIBO prevalence was higher in the NAFLD cohort compared to HC (22/95; 23.2% vs. 1/30; 3.3%, p = 0.014). Patients with NASH had higher SIBO prevalence (6/27; 22.2%) compared to NAFL individuals (8/51; 15.7%), but this difference did not reach statistical significance (p = 0.11). Patients with NASH-associated cirrhosis had a higher SIBO prevalence compared to patients with NAFL (8/17; 47.1% vs. 8/51; 15.7%, p = 0.02), while SIBO prevalence between patients with NASH-associated cirrhosis and NASH was not statistically different (8/17; 47.1% vs. 6/27; 22.2%, p = 0.11). Mean concentration of TNF-α, IL-1β, and IL-6 did not differ among the different groups. Conclusion: The prevalence of SIBO is significantly higher in a cohort of patients with NAFLD compared to healthy controls. Moreover, SIBO is more prevalent in patients with NASH-associated cirrhosis compared to patients with NAFL.
Occurrence and Multidrug Resistance in Strains of Listeria monocytogenes Recovered from the Anaerobic Co-Digestion Sludge Contained in a Single Stage Steel Biodigester: Implications for Antimicrobial Stewardship
Authors: Manyi-Loh Christy Echakachi , Okoh Anthony Ifeanyin , Lues Ryk
Published: March 2023

L. monocytogenes is a zoonotic foodborne pathogen with inherent adaptability to tolerate environmental and physiological stresses, thereby causing severe disease outbreaks. Antibiotic resistant foodborne pathogens are a challenge to the food industry. A total of 18 samples were pooled from a bio-digester co-digesting swine manure/pinewood sawdust, and evaluated for the occurrence of bacterium plus total viable counts using the spread plate method. The recovered bacterial isolates were presumptively identified by growth on selective medium and confirmed by biochemical characterisation, leading to the isolation of 43 L. monocytogenes. The isolates were characterized based on their susceptibility to antibiotics via the Kirby-Bauer disc diffusion technique against a panel of 14 antibiotics. Equally, the multiple antibiotic resistance (MAR) index was calculated, and MAR phenotypes generated. The bacterial counts were between 102 and104 cfu/mL. Complete susceptibility (100%) was demonstrated to ampicillin, gentamicin and sulfamethoxazole, which are the drugs of choice in the treatment of listeriosis. In addition, intermediate sensitivity occurred at 25.58% to cefotaxime, and the highest resistance (51.16%) was exhibited against nalidixic acid. The MAR index ranged from 0 to 0.71. Overall, 41.86% of the Listeria isolates displayed multidrug resistance, with 18 different MAR phenotypes, demonstrating CIP, E, C, TET, AUG, S, CTX, NA, AML, NI as the greatest MAR phenotype. It can be concluded that the isolates yielding MAR > 0.2 originated from the farm, where antibiotics had been in routine use. Therefore, strict monitoring of antibiotics use in the farm is crucial to mitigate further increase in antibiotic resistance amongst these bacterial isolates.
Modes of Action of Biocontrol Agents and Elicitors for sustainable Protection against Bacterial Canker of Tomato
Authors: Benchlih Salma , Esmaeel Qassim , Aberkani Kamal , Tahiri Abdessalem , Belabess Zineb , Lahlali Rachid , Barka Essaid Ait
Published: March 2023

Tomato is one of the world’s most commonly grown and consumed vegetables. However, it can be attacked by the Gram-positive bacterium Clavibacter michiganensis subsp. michiganensis (Cmm), which causes bacterial canker on tomato plants, resulting in significant financial losses in field production and greenhouses worldwide. The current management strategies rely principally on the application of various chemical pesticides and antibiotics, which represent a real danger to the environment and human safety. Plant growth-promoting rhizobacteria (PGPR) have emerged as an attractive alternative to agrochemical crop protection methods. PGPR act through several mechanisms to support plant growth and performance, while also preventing pathogen infection. This review highlights the importance of bacterial canker disease and the pathogenicity of Cmm. We emphasize the application of PGPR as an ecological and cost-effective approach to the biocontrol of Cmm, specifying the complex modes of biocontrol agents (BCAs), and presenting their direct/indirect mechanisms of action that enable them to effectively protect tomato crops. Pseudomonas and Bacillus are considered to be the most interesting PGPR species for the biological control of Cmm worldwide. Improving plants’ innate defense mechanisms is one of the main biocontrol mechanisms of PGPR to manage bacterial canker and to limit its occurrence and gravity. Herein, we further discuss elicitors as a new management strategy to control Cmm, which are found to be highly effective in stimulating the plant immune system, decreasing disease severity, and minimizing pesticide use.
Clinical Utility of the FilmArray® Blood Culture Identification (BCID) Panel for the Diagnosis of Neonatal Sepsis
Authors: Caunedo-Jiménez María , Fernández-Colomer Belén , Fernández-Suárez Jonathan , Arias-Llorente Rosa Patricia , Lareu-Vidal Sonia , Mantecón-Fernández Laura , Solís-Sánchez Gonzalo , Suárez-Rodríguez Marta
Published: March 2023

This prospective single-center study was designed to assess the clinical utility of the FilmArray® blood culture identification (BCID) panel for improving the diagnostic accuracy in neonatal sepsis. Results obtained using the FilmArray® BCID panel were correlated with results of blood culture in all consecutive neonates with suspicion of early-onset (EOS) and late-onset sepsis (LOS) attended in our service over a two-year period. A total of 102 blood cultures from 92 neonates were included, 69 (67.5%) in cases of EOS and 33 (32.3%) in LOS. The FilmArray® BCID panel was performed in negative culture bottles at a median of 10 h of blood culture incubation (IQR 8–20), without differences by the type of sepsis. The FilmArray® BCID panel showed a 66.7% sensitivity, 100% specificity, 100% positive predictive value, and 95.7% negative predictive value. There were four false-negative cases, three of which were Streptococcus epidermidis in neonates with LOS, and there was one case of Granulicatella adiacens in one neonate with EOS. We conclude that the use of the FilmArray® BCID panel in negative blood cultures from neonates with clinical suspicion of sepsis is useful in decision-making of starting or early withdrawal of empirical antimicrobials because of the high specificity and negative predictive values of this assay.
Effect of Wet-Dry Cycles on the Mechanical Performances and Microstructure of Pisha Sandstone
Authors: Zhao Yanbing , Yang Caiqian , Qu Feng , Wu Zhiren , Ding Kejie , Liang Zhishui
Published: March 2023

The effects of the wet–dry cycles on the chemical compositions, microstructure, and mechanical properties of Pisha sandstone were experimentally investigated in the current study. A series of uniaxial compression tests were conducted to validate the deterioration of the mechanical property of specimens after wet–dry cycles. In addition, the evolutions of the mineral compositions and microstructure characteristics were confirmed by X-ray diffraction (XRD) and scanning electron microscope (SEM). Experimental results indicated that with the increase of wet–dry cycles, the mechanical properties of Pisha sandstone gradually decrease. After five wet–dry cycles, the uniaxial compressive strength, elastic modulus, and fracture energy of specimens were reduced by 41.06%, 62.39%, and 31.92%, respectively. The failure mode of the specimen changes from inclined shear failure to peel failure. Compared to the initial specimens, the relative content of primary minerals after five wet–dry cycles declined by 5.94%, and the relative content of clay minerals after five wet–dry cycles increased by 54.33%. Additionally, the porosity of samples exhibits a positive correlation with wet–dry cycles. Compared to the initial specimens, the porosity of specimens after five wet–dry cycles increased by 176.32%. Finally, a prediction model of the correlation between uniaxial compressive strength and porosity is proposed and verified.
Special Issue “Protein Analysis by Mass Spectrometry”
Authors: König Simone
Published: March 2023

When the Molecules Assistant Editors invited me as a Guest Editor for the Special Issue “Protein Analysis by Mass Spectrometry”, I hesitated for several months, not only because of a busy schedule, but also because of the abundance of the literature on the topic [...]
Ultrasound-Assisted Extraction of Protein from Moringa oleifera Seeds and Its Impact on Techno-Functional Properties
Authors: Fatima Khushar , Imran Muhammad , Ahmad Muhammad Haseeb , Khan Muhammad Kamran , Khalid Waseem , AL-Farga Ammar , Alansari Wafa S. , Shamlan Ghalia , Eskandrani Areej A.
Published: March 2023

Plant proteins can be an important alternative to animal proteins subject to minor modification to address sustainability issues. The impact of ultrasound application on the yield, techno-functional properties, and molecular characteristics of protein extracted from Moringa oleifera seeds was studied. For this purpose, a central composite design (CCD) was applied to optimize ultrasound-assisted extraction (UAE) parameters such as amplitude (25–75%), solute-to-solvent ratio (1:10–1:30), and pH (9–13) for obtaining the maximum protein yield. At the optimized conditions of 75% amplitude, 1:20 solute-to-solvent ratio, and 11 pH, a protein yield of 39.12% was obtained in the UAE process. Moreover, the best sonication time at optimized conditions was 20 min, which resulted in about 150% more extraction yield in comparison to conventional extraction (CE). The techno-functional properties, for instance, solubility, water (WHC)- and oil-holding capacity (OHC), and emulsifying and foaming properties of the protein obtained from UAE and CE were also compared. The functional properties revealed high solubility, good WHC and OHC, and improved emulsifying properties for protein obtained from UAE. Although protein from UAE provided higher foam formation, foaming stability was significantly lower.
Study of Influence of Extraction Method on the Recovery Bioactive Compounds from Peel Avocado
Authors: Martínez-Gutiérrez Emir
Published: March 2023

The avocado peel is a waste material from consumption avocado (Persea americana Mill.) with big biotechnology potential. The purpose of the present work was to study the influence of six extraction methods, maceration (M), maceration plus β-cyclodextrin (MβC), solid-state fermentation (SSF), sonication with water or ethanol, wet grinding (WG), wet grinding plus maceration (WGM), on the recovery of bioactive compounds from the avocado peel such as total phenols, epicatechin and chlorogenic acid. The results showed that the extraction method has a significant effect on the content of total phenols, the WGM method obtaining the highest value of total phenols (2143.1 mg GAE/100 g dry weight). Moreover, the results indicated that the extraction method had a significant effect on chlorogenic acid and epicatechin recovery, the WGM method obtaining the highest amount of epicatechin and chlorogenic acid, 181.7 and 244.3 mg/100 g dry matter, respectively. Additionally, the characterization of WGM extract was realized by UPLC-ESI-MS/MS and GC-MS. Thus, the WGM method allowed for obtaining good yields of recovery of phenolic compounds using an accessible technology and a more environment-friendly solvent.
A Comparative Study on CO2-Switchable Foams Stabilized by C22- or C18-Tailed Tertiary Amines
Authors: Liang Meiqing , Zhao Xuezhi , Wang Ji , Feng Yujun
Published: March 2023

The CO2 aqueous foams stabilized by bioresource-derived ultra-long chain surfactants have demonstrated considerable promising application potential owing to their remarkable longevity. Nevertheless, existing research is still inadequate to establish the relationships among surfactant architecture, environmental factors, and foam properties. Herein, two cases of ultra-long chain tertiary amines with different tail lengths, N-erucamidopropyl-N,N-dimethylamine (UC22AMPM) and N-oleicamidopropyl-N,N-dimethylamine (UC18AMPM), were employed to fabricate CO2 foams. The effect of temperature, pressure and salinity on the properties of two foam systems (i.e., foamability and foam stability) was compared using a high-temperature, high-pressure visualization foam meter. The continuous phase viscosity and liquid content for both samples were characterized using rheometry and FoamScan. The results showed that the increased concentrations or pressure enhanced the properties of both foam samples, but the increased scope for UC22AMPM was more pronounced. By contrast, the foam stability for both cases was impaired with increasing salinity or temperature, but the UC18AMPM sample is more sensitive to temperature and salinity, indicating the salt and temperature resistance of UC18AMPM-CO2 foams is weaker than those of the UC22AMPM counterpart. These differences are associated with the longer hydrophobic chain of UC22AMPM, which imparts a higher viscosity and lower surface tension to foams, resisting the adverse effects of temperature and salinity.
Mesoporous Polymeric Ionic Liquid via Confined Polymerization for Laccase Immobilization towards Efficient Degradation of Phenolic Pollutants
Authors: Liang Yu , Chen Xinyan , Zeng Jianli , Ye Junqing , He Bin , Li Wenjin , Sun Jian
Published: March 2023

Laccase immobilization is a promising method that can be used for the recyclable treatment of refractory phenolic pollutants (e.g., chlorophenols) under mild conditions, but the method is still hindered by the trade-off limits of supports in terms of their high specific surface area and rich functional groups. Herein, confined polymerization was applied to create abundant amino-functionalized polymeric ionic liquids (PILs) featuring a highly specific surface area and mesoporous structure for chemically immobilizing laccase. Benefiting from this strategy, the specific surface area of the as-synthesized PILs was significantly increased by 60-fold, from 5 to 302 m2/g. Further, a maximum activity recovery of 82% towards laccase was recorded. The tolerance and circulation of the immobilized laccase under harsh operating conditions were significantly improved, and the immobilized laccase retained more than 84% of its initial activity after 15 days. After 10 cycles, the immobilized laccase was still able to maintain 80% of its activity. Compared with the free laccase, the immobilized laccase exhibited enhanced stability in the biodegradation of 2,4-dichlorophenol (2,4-DCP), recording around 80% (seven cycles) efficiency. It is proposed that the synergistic effect between PILs and laccase plays an important role in the enhancement of stability and activity in phenolic pollutant degradation. This work provides a strategy for the development of synthetic methods for PILs and the improvement of immobilized laccase stability.
Poly-α, β-d, l-Aspartyl-Arg-Gly-Asp-Ser-Based Urokinase Nanoparticles for Thrombolysis Therapy
Authors: Chen Shuangling , Liang Meng , Wu Chengli , Zhang Xiaoyi , Wang Yuji , Zhao Ming
Published: March 2023

The most concerning adverse effects of thrombolytic agents are major bleeding and intracranial hemorrhage due to their short half-life, low fibrin specificity, and high dosage. To alleviate bleeding side effects during thrombolytic therapy which would bring about the risk of aggravation, we try to find a novel biodegradable delivery nanosystem to carry drugs to target the thrombus, reduce the dosage of the drug, and system side effects. A novel urokinase/poly-α, β-d, l-aspartyl-Arg-Gly-Asp-Ser complex (UK/PD-RGDS) was synthesized and simply prepared. Its thrombolytic potency was assayed by the bubble-rising method and in vitro thrombolytic activity by the thrombus clot lysis assay separately. The in vivo thrombolytic activity and bleeding complication were evaluated by a rat model of carotid arteriovenous bypass thrombolysis. The thrombolytic potency (1288.19 ± 155.20 U/mg) of the UK/PD-RGDS complex nano-globule (18–130 nm) was 1.3 times that of commercial UK (966.77 ± 148.08 U/mg). In vivo, the UK/PD-RGDS complex (2000 IU/kg) could reduce the dose of UK by 90% while achieving the equivalent thrombolysis effect as the free UK (20,000 IU/kg). Additionally, the UK/PD-RGDS complex decreased the tail bleeding time compared with UK. The organ distribution of the FITC-UK/PD-RGDS complex was explored in the rat model. The UK/PD-RGDS complex could provide a promising platform to enhance thrombolytic efficacy significantly and reduce the major bleeding degree.
Strong External Electric Fields Reduce Explosive Sensitivity: A Theoretical Investigation into the Reaction Selectivity in NH2NO2∙∙∙NH3
Authors: Ren Fu-De , Liu Ying-Zhe , Wang Xiao-Lei , Qiu Li-Li , Meng Zi-Hui , Cheng Xiang , Li Yong-Xiang
Published: March 2023

Controlling the selectivity of a detonation initiation reaction of explosive is essential to reduce sensitivity, and it seems impossible to reduce it by strengthening the external electric field. To verify this, the effects of external electric fields on the initiation reactions in NH2NO2∙∙∙NH3, a model system of the nitroamine explosive with alkaline additive, were investigated at the MP2/6-311++G(2d,p) and CCSD(T)/6-311++G(2d,p) levels. The concerted effect in the intermolecular hydrogen exchange is characterized by an index of the imaginary vibrations. Due to the weakened concerted effects by the electric field along the −x-direction opposite to the “reaction axis”, the dominant reaction changes from the intermolecular hydrogen exchange to 1,3-intramolecular hydrogen transference with the increase in the field strengths. Furthermore, the stronger the field strengths, the higher the barrier heights become, indicating the lower sensitivities. Therefore, by increasing the field strength and adjusting the orientation between the field and “reaction axis”, not only can the reaction selectivity be controlled, but the sensitivity can also be reduced, in particular under a super-strong field. Thus, a traditional concept, in which the explosive is dangerous under the super-strong external electric field, is theoretically broken. Compared to the neutral medium, a low sensitivity of the explosive with alkaline can be achieved under the stronger field. Employing atoms in molecules, reduced density gradient, and surface electrostatic potentials, the origin of the reaction selectivity and sensitivity change is revealed. This work provides a new idea for the technical improvement regarding adding the external electric field into the explosive system.
Rapid RAFT Polymerization of Acrylamide with High Conversion
Authors: Liu Xuejing , Sun Qiang , Zhang Yan , Feng Yujun , Su Xin
Published: March 2023

Rapid RAFT polymerization can significantly improve production efficiency of PAM with designed molecular structure. This study shows that ideal Reversible Addition–Fragmentation Chain Transfer (RAFT) polymerization of acrylamide is achieved in dimethyl sulfoxide (DMSO) solution at 70 °C. The key to success is the appropriate choice of both a suitable RAFT chain transfer agent (CTA) and initiating species. It is illustrated that dodecyl trithiodimethyl propionic acid (DMPA) is a suitable trithiocarbonate RAFT CTA and is synthesized more easily than other CTAs. Compared to other RAFT processes of polymers, the reaction system shortens reaction time, enhances conversion, and bears all the characteristics of a controlled radical polymerization. The calculation result shows that high concentrations can reduce high conversions, accelerate the reaction rate, and widen molecular weight distributions slightly. This work proposes an excellent approach for rapid synthesis of PAMs with a restricted molecular weight distribution.
Bioactivity-Guided High Performance Counter-Current Chromatography and Following Semi-Preparative Liquid Chromatography Method for Rapid Isolation of Anti-Inflammatory Lignins from Dai Medicinal Plant, Zanthoxylum acanthopodium var. timbor
Authors: Fan Qing-Fei , Zhou Lan , Gongpan Pian-Chou , Lu Chuan-Li , Chang Hua , Xiang Xun
Published: March 2023

The development of Dai medicine is relatively slow, and Zanthoxylum has great economic and medicinal value. It is still difficult to obtain medicinal components from the low-polarity parts of Zanthoxylum belonging to Dai medicine. In this study, we introduced one simple and quick strategy of separating target compounds from the barks of Z. acanthopodium var. timbor by high-performance countercurrent chromatography (HPCCC) with an off-line anti-inflammatory activity screening mode. The development of this strategy was based on the TLC-based generally useful estimation of solvent systems (GUESS) method and HPCCC in combination. This paper presented a rapid method for obtaining target anti-inflammatory compounds. Three lignins were enriched by HPCCC with an off-line inhibition mode of nitric oxide production in lipopolysaccharide-stimulated RAW264.7 macrophage cells, using petroleum ether–ethyl acetate–methanol–water (3:2:3:2) as the solvent system. The results showed that this method was simple and practical and could be applied to trace the anti-inflammatory components of the low-polarity part in Dai medicine.
Neuroprotective and Antiherpetic Properties of Polyphenolic Compounds from Maackia amurensis Heartwood
Authors: Tarbeeva Darya V. , Berdyshev Dmitry V. , Pislyagin Evgeny A. , Menchinskaya Ekaterina S. , Kim Natalya Y. , Kalinovskiy Anatoliy I. , Krylova Natalya V. , Iunikhina Olga V. , Persiyanova Elena V. , Shchelkanov Mikhail Y. , Grigorchuk Valeria P. , Aminin Dmitry L. , Fedoreyev Sergey A.
Published: March 2023

In this study, we isolated a new isoflavanostilbene maackiapicevestitol (1) as a mixture of two stable conformers 1a and 1b as well as five previously known dimeric and monomeric stilbens: piceatannol (2), maackin (3), scirpusin A (4), maackiasine (5), and maackolin (6) from M. amurensis heartwood, using column chromatography on polyamide, silicagel, and C-18. The structures of these compounds were elucidated by NMR, HR-MS, and CD techniques. Maksar® obtained from M. amurensis heartwood and polyphenolics 1–6 possessed moderate anti-HSV-1 activity in cytopathic effect (CPE) inhibition and RT-PCR assays. A model of PQ-induced neurotoxicity was used to study the neuroprotective potential of polyphenolic compounds from M. amurensis. Maksar® showed the highest neuroprotective activity and increased cell viability by 18% at a concentration of 10 μg/mL. Maackolin (6) also effectively increased the viability of PQ-treated Neuro-2a cells and the value of mitochondrial membrane potential at concentrations up to 10 μΜ. Maksar® and compounds 1–6 possessed higher FRAP and DPPH-scavenging effects than quercetin. However, only compounds 1 and 4 at concentrations of 10 μM as well as Maksar® (10 μg/mL) statistically significantly reduced the level of intracellular ROS in PQ-treated Neuro-2a cells.
Determination of the Major By-Products of Citrus hystrix Peel and Their Characteristics in the Context of Utilization in the Industry
Authors: Lubinska-Szczygeł Martyna , Kuczyńska-Łażewska Anna , Rutkowska Małgorzata , Polkowska Żaneta , Katrich Elena , Gorinstein Shela
Published: March 2023

Kaffir lime (Citrus hystrix) is a popular citrus in Southeast Asia. Despite the growing interest in the peel of the fruit, the leaves are the most frequently used part of the fruit. The aim of the study was to determine the main by-products of the peel, such as pectins, minerals, essential oil, and bioactive compounds, and to evaluate the possibility of using them in various branches of industry. In the study of the essential oil obtained by hydrodistillation performed using the TGA chromatography technique (GC-MS), sabinene (31.93%), β-pinene (26%), and limonene (19%) were selected as the most abundant volatile compounds. Nine microelements (Fe, Zn, Cu, Mn, Co, Ni, Cr, Mo, and V), four macroelements (Mg, Ca, K, and Na), and seven ballast substances (Cd, Hg, Pb, Al, V, Sr, and Pt) were also determined using the microwave plasma-atomic emission spectrometry technique (MP-AES). In the case of microelements, iron 32.72 ± 0.39 mg/kg DW (dry weight) had the highest concentration. In the case of macroelements, the calcium content was 9416 ± 34 mg/kg DW. Optimization of the pectin extraction was also performed by selecting citric acid and obtaining a yield of 7.6–17.6% for acid extraction and 9.9–28.2% for ultrasound-assisted extraction (UAE), depending on the temperature used. The obtained pectins were characterized by the degree of methylation, galacturonic acid content, 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging, and DSC (differential scanning calorimetry) analysis. Among bioactive compounds, the contents of polyphenols (22.63 ± 2.12 mg GAE/g DW), flavonoids (2.72 ± 0.25 mg CE/g DW, vitamin C (2.43 ± 0.19 mg Asc), xantoproteins + carotenes (53.8 ± 4.24 ug), anthocyanins (24.8 ± 1.8 mg CGE/kg DW), and chlorophylls A and B (188.5 ± 8.1, 60.4 ± 3.23 µg/g DW) were evaluated. Antioxidant capacity using (cupric ion-reducing antioxidant capacity) CUPRAC and DPPH assays was also provided with the results of 76.98 ± 8.1, and 12.01 ± 1.02 µmol TE/g DW, respectively.
Cripowellins Pause Plasmodium falciparum Intraerythrocytic Development at the Ring Stage
Authors: Butler Joshua H. , Painter Heather J. , Bremers Emily K. , Krai Priscilla , Llinás Manuel , Cassera Maria B.
Published: March 2023

Cripowellins from Crinum erubescens are known pesticidal and have potent antiplasmodial activity. To gain mechanistic insights to this class of natural products, studies to determine the timing of action of cripowellins within the asexual intraerythrocytic cycle of Plasmodium falciparum were performed and led to the observation that this class of natural products induced reversible cytostasis in the ring stage within the first 24 h of treatment. The transcriptional program necessary for P. falciparum to progress through the asexual intraerythrocytic life cycle is well characterized. Whole transcriptome abundance analysis showed that cripowellin B “pauses” the transcriptional program necessary to progress through the intraerythrocytic life cycle coinciding with the lack of morphological progression of drug treated parasites. In addition, cripowellin B-treated parasites re-enter transcriptional progression after treatment was removed. This study highlights the use of cripowellins as chemical probes to reveal new aspects of cell cycle progression of the asexual ring stage of P. falciparum which could be leveraged for the generation of future antimalarial therapeutics.
Tsaokoic Acid: A New Bicyclic Nonene from the Fruits of Amomum tsao-ko with Acetylcholinesterase Inhibitory Activity
Authors: Kim Hyunyoung , Lee Hwaryeong , Jung Hee Jin , Noh Sang Gyun , Youn Isoo , Kwak Hyunkyung , Lee Yeju , Nam Sang-Jip , Kang Soosung , Chung Hae Young , Seo Eun Kyoung
Published: March 2023

A new bicyclic nonene, tsaokoic acid (1), was isolated from the fruits of Amomum tsao-ko, together with three known compounds (2–4). The structure of 1 was elucidated by analyzing spectroscopic data including 1D and 2D NMR spectra and compounds 2–4 were identified as tsaokoin, vanillin, and tsaokoarylone, respectively, by comparing their NMR spectra with previously reported data. Compounds 1–4 showed possible inhibitory activity against acetylcholinesterase (AChE) in silico molecular docking simulations. They were submitted to in vitro assay system and exhibited moderate inhibitory activity with IC50 values of 32.78, 41.70, 39.25, and 31.13 μM, respectively.
Prioritization and Optimal Location of Hydrogen Fueling Stations in Seoul: Using Multi-Standard Decision-Making and ILP Optimization
Authors: Kim Kyeong Ryong , Cho Jae Hyung
Published: March 2023

Thus far, the adoption of hydrogen fuel cell vehicles (HCEVs) has been hampered by the lack of hydrogen fueling infrastructure. This study aimed to determine the optimal location and prioritization of hydrogen fueling stations (HFSs) in Seoul by utilizing a multi-standard decision-making approach and optimization method. HFS candidate sites were evaluated with respect to relevant laws and regulations. Key factors such as safety, economy, convenience, and demand for HCEVs were considered. Data were obtained through a survey of experts in the fields of HCEV and fuel cells, and the Analytic Hierarchy Process method was applied to prioritize candidate sites. The optimal quantity and placement of HFSs was then obtained using optimization software, based on the acceptable travel time from intersections of popular roads in Seoul. Our findings suggest that compliance with legal safety regulations is the most important factor when constructing HFSs. Furthermore, sensitivity analysis revealed that the hydrogen supply cost currently holds the same weight as other elements. The study highlights the importance of utilizing a multi-standard decision-making approach and optimization methods when determining the optimal location and prioritization of HFSs and can help develop a systematic plan for the nationwide construction of HFSs in South Korea.
Analysis of the Shear Stresses in a Filling Line of Parenteral Products: The Role of Tubing
Authors: Moino Camilla , Scutellà Bernadette , Bellini Marco , Bourlès Erwan , Boccardo Gianluca , Pisano Roberto
Published: March 2023

Parenteral products appear to be sensitive to process conditions in bioprocessing steps, such as interfacial stress and shear stress. The combination of these elements is widely believed and proven to influence product stability, but the defined roles of these players in the product damage process have not yet been identified. The present work addresses a current industrial problem, by focusing on the analysis of shear stress on protein-based therapeutics flowing in tubing by means of Computational Fluid Dynamics simulations. The purpose of this article is not to pinpoint the mechanism triggering the damage of the product, but it represents the first step towards wider experimental investigations and introduces a new strategy to quantify the average shear stress. The field of scale-down approaches, used to scale the commercial process down to the laboratory level, is also explored. Since quality control is critical in the pharmaceutical realm, it is essential that the scale-down approach preserves the same stress exposure as the commercial scale, which in the present work is considered to be that resulting from shear effects. Therefore, a new approach for scaling down the commercial process is proposed, which has been compared with traditional approaches and shown to provide greater representativeness between the two scales.
S-Wave Velocity Forecasting Using Drill Cuttings and Deep Hybrid Neural Networks: A Case Study on a Tight Glutenite Reservoir in Mahu Sag, Junggar Basin
Authors: Xiao Fengchao , Li Xuechen , Zhang Shicheng
Published: March 2023

S-wave velocity (Vs) is a critical petrophysical parameter for reservoir characterization. It is desirable to predict Vs based on conventional logging data, but the logging cost is high. Therefore, a deep hybrid neural network coupling the convolutional neural network (CNN), Stacked gated recurrent unit (SGRU) is proposed to predict the Vs, where the inputs to the model are drill cutting features. In the proposed CNN-SGRU hybrid model, CNN is adopted to capture the spatial features from the input data, and SGRU is used to extract the temporal patterns of variation from both the forward and backward directions. To illustrate the prediction effect, the glutenite reservoir in the Baikouquan Formation of Mahu Sag, Junggar Basin is taken as an example. Mineral and pore information of drill cuttings, including siliciclastic content, clay content, quartz content, and void area ratio is chosen as the input data of the CNN-SGRU hybrid model. Three indices are used to quantitatively evaluate the prediction performance, including Mean absolute percentage error (MAPE), Root mean square error (RMSE), and Mean absolute error (MAE). The results show that the prediction accuracy of the proposed model is higher than that of the Xu-White model, CNN, and GRU. Furthermore, the results indicate that drill cuttings can replace logging data to predict Vs.
On the Importance of Model Selection for CFD Analysis of High Temperature Gas-Solid Reactive Flow; Case Study: Post Combustion Chamber of HIsarna Off-Gas System
Authors: Hosseini Ashkan , Hage Johannes L. T. , Meijer Koen , Offerman Erik , Yang Yongxiang
Published: March 2023

In this paper a CFD analysis of HIsarna off-gas system for post combustion of CO-H2-carbon particle mixture is presented to evaluate the effect of different sub-models and parameters on the accuracy of predictions and simulation time. The effects of different mesh type, mesh grid size, radiation models, turbulent models, kinetic mechanism, turbulence chemistry interaction models, including and excluding gas-solid reactions, number of reactive solid particles are investigated in detail. Based on the accuracy of the predictions and agreement with counterpart measured values, the best combination is selected and conclusions are derived. It was found that radiation and turbulence chemistry interaction model have a major effect on the temperature and composition profile prediction along the studied off-gas system, compared to the variations in other models. The effect of these two models becomes even more evident when the temperature and fuel content of the flue gas are high.
Review on Energy Conservation of Construction Machinery for Pumping Concrete
Authors: Liu Huiyong , Zhao Qing
Published: March 2023

The excessive consumption of fossil fuel, energy shortage and global warming along with environmental deterioration have increasingly become a global issue. In order to deal with the energy crisis, energy conservation has been developed and applied in vehicles and construction machineries, i.e., excavators, loaders and forklifts. Due to the shortcoming of low efficiency, high-energy consumption and bad exhaust, the energy conservation of construction machinery for pumping concrete is necessary and urgent. This paper aims to carry out a review on energy conservation of construction machinery for pumping concrete. The research methodology comprises a quantitative analysis method and literature investigation method. First, the structure and working principle of construction machinery for pumping concrete are expounded, and energy consumption ways of construction machinery for pumping concrete are analyzed. Then, research developments in the energy conservation of construction machinery for pumping concrete are summarized. Finally, challenges with the energy conservation of construction machinery for pumping concrete are presented.
High Step-Up Thirteen Level Switched-Capacitor Inverter Topology for HFAC Power System
Authors: Algadair Badr Saleh A. , Elmorshedy Mahmoud F. , Mohamed Ali Jagabar Sathik , Almakhles Dhafer , Prem Ponnusamy
Published: March 2023

This article proposes two new high-frequency, thirteen-level switched capacitor inverter topologies. Compared with the counterpart existing topologies, which were recently published, the switch count, capacitor voltage, and voltage stress on the switch are reduced. In addition, the proposed topologies have inherited merits such as six-time voltage boosting levels and self-balanced capacitor voltages as well. The capacitor ripple voltages and the proposed topology performance are analyzed. The prototype setup is developed for 500W, and the results are verified. The maximum efficiency is approximately equal to 97.5% for simulation. Furthermore, it is approximately equal to 96.1% for experimental, and both are measured at an output power of 500 W. Finally, the characteristics of the proposed topologies are tested under several scenarios, such as modulation index variations and load changes, and the findings are reported.
Tools for Optimization of Biomass-to-Energy Conversion Processes
Authors: Batista Ranielly M. , Converti Attilio , Pappalardo Juliano , Benachour Mohand , Sarubbo Leonie A.
Published: March 2023

Biomasses are renewable sources used in energy conversion processes to obtain diverse products through different technologies. The production chain, which involves delivery, logistics, pre-treatment, storage and conversion as general components, can be costly and uncertain due to inherent variability. Optimization methods are widely applied for modeling the biomass supply chain (BSC) for energy processes. In this qualitative review, the main aspects and global trends of using geographic information systems (GISs), linear programming (LP) and neural networks to optimize the BSC are presented. Modeling objectives and factors considered in studies published in the last 25 years are reviewed, enabling a broad overview of the BSC to support decisions at strategic, tactical and operational levels. Combined techniques have been used for different purposes: GISs for spatial analyses of biomass; neural networks for higher heating value (HHV) correlations; and linear programming and its variations for achieving objectives in general, such as costs and emissions reduction. This study reinforces the progress evidenced in the literature and envisions the increasing inclusion of socio-environmental criteria as a challenge in future modeling efforts.
„I Die, but I Thank You…!“ Leipzig Mission at Akeri 1896, Squeezed between Its African Addressees and German Colonial Military
Authors: Fischer Moritz
Published: March 2023

The following case study clarifies how these three different functions of mission are discursively entangled with one another. Mission as a bridge-builder (between people, cultures, and religions of different origin), as a traitor (cooperating with corrupt colonial and imperial powers), and as a victim (finding misery and death on the mission field). Each of these three terms (bridge-builder, traitor, victim) is, to an extent, applicable to the events that took place during the night of 19–20 October 1896 in Akeri on the slopes of Mount Meru (former German East Africa, today Tanzania). Using the concept of entanglement history, I will analyze the death of two young German missionaries of the Lutheran Leipzig Mission, “caught in the crossfire” between the African community to be outreached and the German colonial military. We will see how various symbolic systems collide in the year 1896 at Akeri. The systems are represented by: (1) German Lutheran missions activities; (2) A German colonial and military expedition; and (3) The resistance of African Maasai societies’ leadership. “Akeri 1896” (I will continue to refer to this event specifically as “Akeri 1896” throughout the article) had become in the following 100 years a complex entanglement of metaphoric meanings. The same event can be a placeholder for victory, for defeat, for disaster, for martyrdom, for Christ-centredness (of the missionaries in their own perception), as well as for evil-centredness (the Africans in their perception of the Western foreigners).