bluuebunny/crossref_metadata_2025 huggingface dataset:
Lack of the α1,3-Fucosyltransferase Gene (Osfuct) Affects Anther Development and Pollen Viability in Rice
Authors: Sim Joon-Soo , Kesawat Mahipal Singh , Kumar Manu , Kim Su-Yeon , Mani Vimalraj , Subramanian Parthiban , Park Soyoung , Lee Chang-Muk , Kim Seong-Ryong , Hahn Bum-Soo
Published: April 2018

N-linked glycosylation is one of the key post-translational modifications. α1,3-Fucosyltransferase (OsFucT) is responsible for transferring α1,3-linked fucose residues to the glycoprotein N-glycan in plants. We characterized an Osfuct mutant that displayed pleiotropic developmental defects, such as impaired anther and pollen development, diminished growth, shorter plant height, fewer tillers, and shorter panicle length and internodes under field conditions. In addition, the anthers were curved, the pollen grains were shriveled, and pollen viability and pollen number per anther decreased dramatically in the mutant. Matrix-assisted laser desorption/ionization time-of-flight analyses of the N-glycans revealed that α1,3-fucose was lacking in the N-glycan structure of the mutant. Mutant complementation revealed that the phenotype was caused by loss of Osfuct function. Transcriptome profiling also showed that several genes essential for plant developmental processes were significantly altered in the mutant, including protein kinases, transcription factors, genes involved in metabolism, genes related to protein synthesis, and hypothetical proteins. Moreover, the mutant exhibited sensitivity to an increased concentration of salt. This study facilitates a further understanding of the function of genes mediating N-glycan modification and anther and pollen development in rice.
CSI Frequency Domain Fingerprint-Based Passive Indoor Human Detection
Authors: Han Chong , Tan Qingqing , Sun Lijuan , Zhu Hai , Guo Jian
Published: April 2018

Passive indoor personnel detection technology is now a hot topic. Existing methods have been greatly influenced by environmental changes, and there are problems with the accuracy and robustness of detection. Passive personnel detection based on Wi-Fi not only solves the above problems, but also has the advantages of being low cost and easy to implement, and can be better applied to elderly care and safety monitoring. In this paper, we propose a passive indoor personnel detection method based on Wi-Fi, which we call FDF-PIHD (Frequency Domain Fingerprint-based Passive Indoor Human Detection). Through this method, fine-grained physical layer Channel State Information (CSI) can be extracted to generate feature fingerprints so as to help determine the state in the scene by matching online fingerprints with offline fingerprints. In order to improve accuracy, we combine the detection results of three receiving antennas to obtain the final test result. The experimental results show that the detection rates of our proposed scheme all reach above 90%, no matter whether the scene is human-free, stationary or a moving human presence. In addition, it can not only detect whether there is a target indoors, but also determine the current state of the target.
Evaluating Penetration Ability of Plodia interpunctella (Hübner) (Lepidoptera: Pyralidae) Larvae into Multilayer Polypropylene Packages
Authors: Scheff Deanna S. , Sehgal Blossom , Subramanyam Bhadriraju
Published: April 2018

The larvae of the Indian meal moth, Plodia interpunctella (Hübner), can invade or penetrate packaging materials and infest food products. Energy bars with three polypropylene packaging types were challenged with eggs (first instars), third instars, and fifth instars of P. interpunctella to determine package resistance at 28 °C and 65% r.h. The packing types were also challenged with two male and two female pupae of P. interpunctella under similar conditions in order to determine which package provided the greatest protection against larval penetration. Samples infested with eggs, third instars, and pupae were evaluated after 21 days and 42 days to count the number of larvae, pupae, and adults found inside the packages. Packages challenged with fifth instars were observed after 21 days to count the number of larvae, pupae, and adults inside each package. The number and diameter of the holes were determined in each package, followed by the amount of damage sustained to the energy bar. Third and fifth instars showed a higher tendency to penetrate all of the packaging types. First instars showed a reduction in package penetration ability compared with third and fifth instars. The increase in exposure time resulted in an increase in the damage sustained to the energy bars. Among packaging types, the thickest package (Test A) was most resilient to penetration by all of the larval stages. In conclusion, energy bar manufacturers need to invest more effort into improving packaging designs, creating thicker gauge films, or advancing odor barrier technology, in order to prevent penetration and infestation by P. interpunctella larvae.
Cerebral Hemodynamic Influences in Task-Related Functional Magnetic Resonance Imaging and Near-Infrared Spectroscopy in Acute Sport-Related Concussion: A Review
Authors: Forcione Mario , Colonnese Claudio , Belli Antonio
Published: April 2018

One of the challenges of managing athletes with sport-related concussion (SRC) is guiding them to a safe return to play. A potential biomarker for use in the clinical assessment of recovery is the analysis of brain activation patterns during task-related functional Magnetic Resonance Imaging (fMRI). However, fMRI studies have provided conflicting results regarding what is pathological. An element that can contribute to this disagreement are hemodynamic impairments of the brain that follow a concussion. A functional neuroimaging technique based on the optical properties of brain tissue—called functional near-infrared spectroscopy (fNIRS)—can be used to evaluate SRC athletes, partially taking into consideration these brain hemodynamic impairments. However, so far, fNIRS has not been extensively used in concussion. In this critical review, there is a description of the main fMRI results involving the neocortex in acutely concussed patients, the influences of hemodynamic impairments on fMRI and fNIRS and the advantages and disadvantages of fNIRS to limit this influence.
Ten Years’ Experience with the CYP2D6 Activity Score: A Perspective on Future Investigations to Improve Clinical Predictions for Precision Therapeutics
Authors: Gaedigk Andrea , Dinh Jean , Jeong Hyunyoung , Prasad Bhagwat , Leeder J.
Published: April 2018

The seminal paper on the CYP2D6 Activity Score (AS) was first published ten years ago and, since its introduction in 2008, it has been widely accepted in the field of pharmacogenetics. This scoring system facilitates the translation of highly complex CYP2D6 diplotype data into a patient’s phenotype to guide drug therapy and is at the core of all CYP2D6 gene/drug pair guidelines issued by the Clinical Pharmacogenetics Implementation Consortium (CPIC). The AS, however, only explains a portion of the variability observed among individuals and ethnicities. In this review, we provide an overview of sources in addition to CYP2D6 genotype that contribute to the variability in CYP2D6-mediated drug metabolism and discuss other factors, genetic and non-genetic, that likely contribute to the observed variability in CYP2D6 enzymatic activity.
Environmental Conservation in the Tibetan Plateau Region: Lessons for China’s Belt and Road Initiative in the Mountains of Central Asia
Authors: Foggin J. Marc
Published: April 2018

Environmental conservation has developed significantly in China over the past 20 years, including more collaborative approaches and recent advances in establishing a national parks system. This study reviews the development of protected areas in the headwaters of the Yangtze River, drawing lessons from experiences of community development and co-management approaches. Community engagement and participation in developing localized plans for natural resource utilization and conservation have been critical features of successful ventures. Government programs and policies, the emergence of grassroots civil society, and the development of herders’ cooperatives and protected areas, are all tracked, each pointing towards the significant value of inclusive biodiversity conservation approaches for meeting broadly agreed development agendas, such as achieving the Sustainable Development Goals by 2030. Observations from the vast, high, arid, and semi-arid lands of the Tibetan Plateau are then considered in light of China’s Belt and Road Initiative, which is bringing vast financial and technical resources to the world. Special attention is given to applying the lessons that have been learned in China to the mountains of Central Asia, globally recognized as a biodiversity hotspot and a water tower for large downstream populations. Keeping local people at the heart of conservation is deemed fundamentally important.
A New Model for Optimal Mechanical and Thermal Performance of Cement-Based Partition Wall
Authors: Huang Shiping , Hu Mengyu , Huang Yonghui , Cui Nannan , Wang Weifeng
Published: April 2018

The prefabricated cement-based partition wall has been widely used in assembled buildings because of its high manufacturing efficiency, high-quality surface, and simple and convenient construction process. In this paper, a general porous partition wall that is made from cement-based materials was proposed to meet the optimal mechanical and thermal performance during transportation, construction and its service life. The porosity of the proposed partition wall is formed by elliptic-cylinder-type cavities. The finite element method was used to investigate the mechanical and thermal behaviour, which shows that the proposed model has distinct advantages over the current partition wall that is used in the building industry. It is found that, by controlling the eccentricity of the elliptic-cylinder cavities, the proposed wall stiffness can be adjusted to respond to the imposed loads and to improve the thermal performance, which can be used for the optimum design. Finally, design guidance is provided to obtain the optimal mechanical and thermal performance. The proposed model could be used as a promising candidate for partition wall in the building industry.
π-Bridge Effect on Symmetric Carbazole-Based Small Molecules for Realizing Ultraviolet Fluorescent Emission
Authors: Liu Siyang , Lin Pengju , Niu Fangfang , Zeng Pengju , Zhang Bin
Published: April 2018

A series of symmetric carbazole derivatives (CzP-H, CzP-CN, CzP-Me, and CzP-OMe), which comprise electron-donating and electron-drawing groups appending on a phenyl core, was synthesized and characterized in detail. These compounds exhibit excellent thermal stabilities, with thermal decomposition temperatures exceeding 400 °C. From the fluorescent spectra in film, CzP-H, CzP-Me, and CzP-OMe showed UV to blue-violet emission, with peaks at 396 nm, 402 nm, and 392 nm, respectively. The E00 energies of CzP-H, CzP-CN, CzP-Me, and CzP-OMe were 3.39 eV, 2.83 eV, 3.50 eV, and 3.35 eV, respectively. From the electrochemical measurements, the highest occupied molecular orbital (HOMOs) energy levels were −5.30 eV, −5.64 eV, −5.46 eV, and −5.24 eV for CzP-H, CzP-CN, CzP-Me, and CzP-OMe, respectively. Through calculations from HOMO energy levels and E00 energies, the lowest unoccupied molecular orbital (LUMOs) energy levels of CzP-H, CzP-CN, CzP-Me, and CzP-OMe were −1.91 eV, −2.81 eV, −1.96 eV, and −1.89 eV, respectively. Therefore, the introduction of different substitutes in phenyl cores would distinctly affect the photophysical properties. These results indicate that the prepared carbazole derivatives could be potential candidates for realizing ultraviolet or blue-violet emission.
Fabrication of FeAl Intermetallic Foams by Tartaric Acid-Assisted Self-Propagating High-Temperature Synthesis
Authors: Karczewski Krzysztof , Stępniowski Wojciech J. , Salerno Marco
Published: April 2018

Iron aluminides are intermetallics with interesting applications in porous form thanks to their mechanical and corrosion resistance properties. However, making porous forms of these materials is not easy due to their high melting points. We formed FeAl foams by elemental iron and aluminum powders sintering with tartaric acid additive. Tartaric acid worked as an in situ gas-releasing agent during the self-propagating high-temperature synthesis of FeAl intermetallic alloy, which was confirmed by X-ray diffraction measurements. The porosity of the formed foams was up to 36 ± 4%. In the core of the sample, the average equivalent circle diameter was found to be 47 ± 20 µm, while on the surface, it was 35 ± 16 µm; thus, the spread of the pore size was smaller than reported previously. To investigate functional applications of the formed FeAl foam, the pressure drop of air during penetration of the foam was examined. It was found that increased porosity of the material increased the flow of the air through the metallic foam.
A Novel Distributed Economic Model Predictive Control Approach for Building Air-Conditioning Systems in Microgrids
Authors: Zhang Xinan , Wang Ruigang , Bao Jie
Published: April 2018

With the penetration of grid-connected renewable energy generation, microgrids are facing stability and power quality problems caused by renewable intermittency. To alleviate such problems, demand side management (DSM) of responsive loads, such as building air-conditioning system (BACS), has been proposed and studied. In recent years, numerous control approaches have been published for proper management of single BACS. The majority of these approaches focus on either the control of BACS for attenuating power fluctuations in the grid or the operating cost minimization on behalf of the residents. These two control objectives are paramount for BACS control in microgrids and can be conflicting. As such, they should be considered together in control design. As individual buildings may have different owners/residents, it is natural to control different BACSs in an autonomous and self-interested manner to minimize the operational costs for the owners/residents. Unfortunately, such “selfish” operation can result in abrupt and large power fluctuations at the point of common coupling (PCC) of the microgrid due to lack of coordination. Consequently, the original objective of mitigating power fluctuations generated by renewable intermittency cannot be achieved. To minimize the operating costs of individual BACSs and simultaneously ensure desirable overall power flow at PCC, this paper proposes a novel distributed control framework based on the dissipativity theory. The proposed method achieves the objective of renewable intermittency mitigation through proper coordination of distributed BACS controllers and is scalable and computationally efficient. Simulation studies are carried out to illustrate the efficacy of the proposed control framework.
Pharmacokinetic and Tissue Distribution of Fucoidan from Fucus vesiculosus after Oral Administration to Rats
Authors: Pozharitskaya Olga N. , Shikov Alexander N. , Faustova Natalya M. , Obluchinskaya Ekaterina D. , Kosman Vera M. , Vuorela Heikki , Makarov Valery G.
Published: April 2018

Fucus vesiculosus L., known as bladderwrack, belongs to the brown seaweeds, which are widely distributed throughout northern Russia, Atlantic shores of Europe, the Baltic Sea, Greenland, the Azores, the Canary Islands, and shores of the Pacific Ocean. Fucoidan is a major fucose-rich sulfated polysaccharide found in Fucus (F.) vesiculosus. The pharmacokinetic profiling of active compounds is essential for drug development and approval. The aim of the study was to evaluate the pharmacokinetics and tissue distribution of fucoidan in rats after a single-dose oral administration. Fucoidan was isolated from F. vesiculosus. The method of measuring anti-activated factor X (anti-Xa) activity by amidolytic assay was used to analyze the plasma and tissue concentrations of fucoidan. The tissue distribution of fucoidan after intragastric administration to the rats was characterized, and it exhibited considerable heterogeneity. Fucoidan preferentially accumulates in the kidneys (AUC0–t = 10.74 µg·h/g; Cmax = 1.23 µg/g after 5 h), spleen (AUC0–t = 6.89 µg·h/g; Cmax = 0.78 µg/g after 3 h), and liver (AUC0–t = 3.26 µg·h/g; Cmax = 0.53 µg/g after 2 h) and shows a relatively long absorption time and extended circulation in the blood, with a mean residence time (MRT) = 6.79 h. The outcome of this study provides additional scientific data for traditional use of fucoidan-containing plants and offers tangible support for the continued development of new effective pharmaceuticals using fucoidan.
Microstructure and Properties of Semi-solid ZCuSn10P1 Alloy Processed with an Enclosed Cooling Slope Channel
Authors: Li Yongkun , Zhou Rongfeng , Li Lu , Xiao Han , Jiang Yehua
Published: April 2018

Semi-solid ZCuSn10P1 alloy slurry was fabricated by a novel enclosed cooling slope channel (ECSC). The influence of pouring length of ECSC on the microstructures of ZCuSn10P1 alloy semi-solid slurry was studied with an optical microscope an optical microscope (OM), scanning electron microscope (SEM), X-ray diffraction (XRD) and energy dispersive spectrometer (EDS). Liquid squeeze casting and semi-solid squeeze casting were performed under the same forming conditions, and the microstructure and properties were compared. The results show that primary α-Cu phase gradually evolved from dendrites to worm-like or equiaxed grains under the chilling action of the inner wall of the ECSC. The mass fraction of tin in the primary α-Cu phase increased from 5.85 to 6.46 after the ECSC process, and intergranular segregation was effectively suppressed. The finest microstructure can be obtained at 300 mm pouring length of ECSC; the equivalent diameter is 46.6 μm and its shape factor is 0.73. The average ultimate tensile strength and average elongation of semi-solid squeeze casting ZCuSn10P1 alloy reached 417 MPa and 12.6%, which were improved by 22% and 93%, respectively, as compared to that of liquid squeeze casting.
Enhancing the Hardness and Compressive Response of Magnesium Using Complex Composition Alloy Reinforcement
Authors: Tun Khin Sandar , Zhang Yuming , Parande Gururaj , Manakari Vyasaraj , Gupta Manoj
Published: April 2018

The present study reports the development of new magnesium composites containing complex composition alloy (CCA) particles. Materials were synthesized using a powder metallurgy route incorporating hybrid microwave sintering and hot extrusion. The presence and variation in the amount of ball-milled CCA particles (2.5 wt %, 5 wt %, and 7.5 wt %) in a magnesium matrix and their effect on the microstructure and mechanical properties of Mg-CCA composites were investigated. The use of CCA particle reinforcement effectively led to a significant matrix grain refinement. Uniformly distributed CCA particles were observed in the microstructure of the composites. The refined microstructure coupled with the intrinsically high hardness of CCA particles (406 HV) contributed to the superior mechanical properties of the Mg-CCA composites. A microhardness of 80 HV was achieved in a Mg-7.5HEA (high entropy alloy) composite, which is 1.7 times higher than that of pure Mg. A significant improvement in compressive yield strength (63%) and ultimate compressive strength (79%) in the Mg-7.5CCA composite was achieved when compared to that of pure Mg while maintaining the same ductility level. When compared to ball-milled amorphous particle-reinforced and ceramic-particle-reinforced Mg composites, higher yield and compressive strengths in Mg-CCA composites were achieved at a similar ductility level.
A Method Based on Semi-Solid Forming for Eliminating Coarse Dendrites and Shrinkage Porosity of H13 Tool Steel
Authors: Guo Yifeng , Liu Weifeng , Sun Mingyue , Xu Bin , Li Dianzhong
Published: April 2018

A method called forging solidifying metal (FSM), which is applied for eliminating coarse dendrites and shrinkage porosity defects of ferrous alloys was proposed based on semi-solid forming technology (SSF). To verify its feasibility, the effects of liquid fraction (FL) on the microstructure of the deformed H13 steel were investigated experimentally. The coarse dendrites structure still existed and cracks appeared when the 0.1/s 50% FSM method was carried out at ~20% FL. What is significantly different from that is, the elimination of the coarse dendrites structure and shrinkage porosity defects became more significant, when this method was conducted at the end of solidification (FL < 10%). The microstructure of H13 steel was significantly refined and also became dense in such condition.
Position-Space-Based Design of a Symmetric Spatial Translational Compliant Mechanism for Micro-/Nano-Manipulation
Authors: Li Haiyang , Hao Guangbo
Published: April 2018

Symmetry enables excellent motion performance of compliant mechanisms, such as minimized parasitic motion, reduced cross-axis coupling, mitigated buckling, and decreased thermal sensitivity. However, most existing symmetric compliant mechanisms are heavily over-constrained due to the fact that they are usually obtained by directly adding over-constraints to the associated non-symmetric compliant mechanisms. Therefore, existing symmetric compliant mechanisms usually have relatively complex structures and relatively large actuation stiffness. This paper presents a position-space-based approach to the design of symmetric compliant mechanisms. Using this position-space-based approach, a non-symmetric compliant mechanism can be reconfigured into a symmetric compliant mechanism by rearranging the compliant modules and adding minimal over-constraints. A symmetric spatial translational compliant parallel mechanism (symmetric XYZ compliant parallel mechanism (CPM)) is designed using the position-space-based design approach in this paper. Furthermore, the actuation forces of the symmetric XYZ CPM are nonlinearly and analytically modelled, which are represented by the given primary translations and the geometrical parameters. The maximum difference, between the nonlinear analytical results and the nonlinear finite element analysis (FEA) results, is less than 2.58%. Additionally, a physical prototype of the symmetric XYZ CPM is fabricated, and the desirable motion characteristics such as minimized cross-axis coupling are also verified by FEA simulations and experimental testing.
Pore Structure and Fractal Characteristics of Niutitang Shale from China
Authors: Xi Zhaodong , Tang Shuheng , Wang Jing , Yi Junjie , Guo Yingying , Wang Kaifeng
Published: April 2018

A suite of shale samples from the Lower Cambrian Niutitang Formation in northwestern Hunan Province, China, were investigated to better understand the pore structure and fractal characteristics of marine shale. Organic geochemistry, mineralogy by X-ray diffraction, porosity, permeability, mercury intrusion and nitrogen adsorption and methane adsorption experiments were conducted for each sample. Fractal dimension D was obtained from the nitrogen adsorption data using the fractal Frenkel-Halsey-Hill (FHH) model. The relationships between total organic carbon (TOC) content, mineral compositions, pore structure parameters and fractal dimension are discussed, along with the contributions of fractal dimension to shale gas reservoir evaluation. Analysis of the results showed that Niutitang shale samples featured high TOC content (2.51% on average), high thermal maturity (3.0% on average), low permeability and complex pore structures, which are highly fractal. TOC content and mineral compositions are two major factors affecting pore structure but they have different impacts on the fractal dimension. Shale samples with higher TOC content had a larger specific surface area (SSA), pore volume (PV) and fractal dimension, which enhanced the heterogeneity of the pore structure. Quartz content had a relatively weak influence on shale pore structure, whereas SSA, PV and fractal dimension decreased with increasing clay mineral content. Shale with a higher clay content weakened pore structure heterogeneity. The permeability and Langmuir volume of methane adsorption were affected by fractal dimension. Shale samples with higher fractal dimension had higher adsorption capacity but lower permeability, which is favorable for shale gas adsorption but adverse to shale gas seepage and diffusion.
Theoretical Study of the β-Cyclodextrin Inclusion Complex Formation of Eugenol in Water
Authors: Alvira Elena
Published: April 2018

The interaction between eugenol and β-cyclodextrin in the presence of water is studied by molecular mechanics and dynamics simulations. A force field model is used in molecular mechanics to determine the interaction energy and the complex configuration at the absolute minimum. The van der Waals term is the main contribution to the total energy, and so directly determines the configuration of the inclusion complex. The formation of inclusion complexes is simulated by molecular dynamics, in which their configurations are deduced from the position probability density that represents the preferred location and orientation of the guest in the simulation. When eugenol approaches from the rims of β-cyclodextrin, it tends to enter the cavity, remain inside for a short period and then exit from it. The guest tends to include the phenyl ring inside the cavity in the most probable configurations. Two inclusion complex configurations are proposed, each with the hydroxyl and methoxyl groups pointing towards one different rim of β-cyclodextrin. The initial guest orientation is the main factor determining these configurations. The model presented in this study reproduces the experimental findings on inclusion complex formation and proposes two possible complex configurations, one previously suggested by different authors.
Chemoresistance to Cancer Treatment: Benzo-α-Pyrene as Friend or Foe?
Authors: Dzobo Kevin , Hassen Naseeha , Senthebane Dimakatso Alice , Thomford Nicholas Ekow , Rowe Arielle , Shipanga Hendrina , Wonkam Ambroise , Parker M. Iqbal , Mowla Shaheen , Dandara Collet
Published: April 2018

Background: Environmental pollution such as exposure to pro-carcinogens including benzo-α-pyrene is becoming a major problem globally. Moreover, the effects of benzo-α-pyrene (BaP) on drug pharmacokinetics, pharmacodynamics, and drug resistance warrant further investigation, especially in cancer outpatient chemotherapy where exposure to environmental pollutants might occur. Method: We report here on the effects of benzo-α-pyrene on esophageal cancer cells in vitro, alone, or in combination with chemotherapeutic drugs cisplatin, 5-flurouracil, or paclitaxel. As the study endpoints, we employed expression of proteins involved in cell proliferation, drug metabolism, apoptosis, cell cycle analysis, colony formation, migration, and signaling cascades in the WHCO1 esophageal cancer cell line after 24 h of treatment. Results: Benzo-α-pyrene had no significant effect on WHCO1 cancer cell proliferation but reversed the effect of chemotherapeutic drugs by reducing drug-induced cell death and apoptosis by 30–40% compared to drug-treated cells. The three drugs significantly reduced WHCO1 cell migration by 40–50% compared to control and BaP-treated cells. Combined exposure to drugs was associated with significantly increased apoptosis and reduced colony formation. Evaluation of survival signaling cascades showed that although the MEK-ERK and Akt pathways were activated in the presence of drugs, BaP was a stronger activator of the MEK-ERK and Akt pathways than the drugs. Conclusion: The present study suggest that BaP can reverse the effects of drugs on cancer cells via the activation of survival signaling pathways and upregulation of anti-apoptotic proteins such as Bcl-2 and Bcl-xL. Our data show that BaP contribute to the development of chemoresistant cancer cells.
HIV Tat/P-TEFb Interaction: A Potential Target for Novel Anti-HIV Therapies
Authors: Asamitsu Kaori , Fujinaga Koh , Okamoto Takashi
Published: April 2018

Transcription is a crucial step in the life cycle of the human immunodeficiency virus type 1 (HIV 1) and is primarily involved in the maintenance of viral latency. Both viral and cellular transcription factors, including transcriptional activators, suppressor proteins and epigenetic factors, are involved in HIV transcription from the proviral DNA integrated within the host cell genome. Among them, the virus-encoded transcriptional activator Tat is the master regulator of HIV transcription. Interestingly, unlike other known transcriptional activators, Tat primarily activates transcriptional elongation and initiation by interacting with the cellular positive transcriptional elongation factor b (P-TEFb). In this review, we describe the molecular mechanism underlying how Tat activates viral transcription through interaction with P-TEFb. We propose a novel therapeutic strategy against HIV replication through blocking Tat action.
Huberine, a New Canthin-6-One Alkaloid from the Bark of Picrolemma huberi
Authors: López Carlos , Pastrana Manuel , Ríos Alexandra , Cogollo Alvaro , Pabón Adriana
Published: April 2018

A new alkaloid, Canthin-6-one, Huberine (1), together with three known compounds including 1-Hydroxy-canthin-6-one (2), Canthin-6-one (3) and stigma sterol (4), were isolated from the stem bark of Picrolemma huberi. The isolation was achieved by chromatographic techniques and the purification was performed on a C18 column using acetonitrile/water (90:10, v/v) with 0.1% formic acid as the mobile phase. The structural elucidation was performed via spectroscopic methods, notably 1D- and 2D-NMR, UV, IR, MS and HRMS. The antiplasmodial activity of the compounds was studied.
Structural Diversity and Biological Activities of Diterpenoids Derived from Euphorbia fischeriana Steud
Authors: Jian Baiyu , Zhang Hao , Liu Jicheng
Published: April 2018

Diterpenoids are the focus of natural product drug discovery because of their great structural diversity and pronounced biological activities. Euphorbia fischeriana Steud is a Chinese traditional medicinal herb for curing edema, ascites, and cancer. This plant contains rich diterpenoids. Based on the carbon skeleton and substituents, it can be classified into thirteen subtypes: ent-abietane, daphnane, tigliane, ingenane, ent-atisane, ent-rosane, ent-kaurene, ent-kaurane, secotigliane, lathyrane, ent-pimarene, isopimarene and dimeric. In this paper, we reviewed the chemical structures and biological activities of 90 diterpenoids isolated from this medicinal herb. We hope that this work can serve as a reference for further research of these diterpenoids and lay the foundation for drug discovery.
Fluorescent Dendritic Micro-Hydrogels: Synthesis, Analysis and Use in Single-Cell Detection
Authors: Christadore Lisa , Grinstaff Mark W. , Schaus Scott E.
Published: April 2018

Hydrogels are of keen interest for a wide range of medical and biotechnological applications including as 3D substrate structures for the detection of proteins, nucleic acids, and cells. Hydrogel parameters such as polymer wt % and crosslink density are typically altered for a specific application; now, fluorescence can be incorporated into such criteria by specific macromonomer selection. Intrinsic fluorescence was observed at λmax 445 nm from hydrogels polymerized from lysine and aldehyde- terminated poly(ethylene glycol) macromonomers upon excitation with visible light. The hydrogel’s photochemical properties are consistent with formation of a nitrone functionality. Printed hydrogels of 150 μm were used to detect individual cell adherence via a decreased in fluorescence. The use of such intrinsically fluorescent hydrogels as a platform for cell sorting and detection expands the current repertoire of tools available.
Dendrimers for Drug Delivery
Authors: Chauhan Abhay Singh
Published: April 2018

Dendrimers have come a long way in the last 25 years since their inception. Originally created as a wonder molecule of chemistry, dendrimer is now in the fourth class of polymers. Dr. Donald Tomalia first published his seminal work on Poly(amidoamine) (PAMAM) dendrimers in 1985. Application of dendrimers as a drug delivery system started in late 1990s. Dendrimers for drug delivery are employed using two approaches: (i) formulation and (ii) nanoconstruct. In the formulation approach, drugs are physically entrapped in a dendrimer using non-covalent interactions, whereas drugs are covalently coupled on dendrimers in the nanoconstruct approach. We have demonstrated the utility of PAMAM dendrimers for enhancing solubility, stability and oral bioavailability of various drugs. Drug entrapment and drug release from dendrimers can be controlled by modifying dendrimer surfaces and generations. PAMAM dendrimers are also shown to increase transdermal permeation and specific drug targeting. Dendrimer platforms can be engineered to attach targeting ligands and imaging molecules to create a nanodevice. Dendrimer nanotechnology, due to its multifunctional ability, has the potential to create next generation nanodevices.
Comparison Study on the Adsorption Capacity of Rhodamine B, Congo Red, and Orange II on Fe-MOFs
Authors: Wei Fuhua , Chen Ding , Liang Zhao , Zhao Shuaiqi
Published: April 2018

Using a microwave-assisted ball-milling approach, Fe-based metal-organic frameworks (Fe-MOFs) were prepared from FeSO4·7H2O and trimesic acid. Scanning electron microscopy, Fourier-transform infrared spectrometry, X-ray, and thermogravimetric analysis were utilized to characterize the thermal stability and structure of the prepared Fe-MOFs. These Fe-MOFs were used to remove organic dyes from aqueous solutions. Specifically, they removed 96.97% of 23.3592 mg/L of Congo red in a 200 mL solution within 300 min of treatment with natural light at 15 °C. Likewise, 88.21 and 70.90% of 22.7527 mg/L of Orange II and 17.8326 mg/L of Rhodamine B, respectively, were removed from 200 mL solutions within 300 min of treatment at 15 °C. At 35 °C, 99.57, 95.98, and 99.38% of 23.3855 mg/L of Congo Red, 22.7365 mg/L of Orange II, and 17.9973 mg/L of Rhodamine B, respectively, were removed from 200 mL solutions within 300 min of treatment. The adsorption kinetics were investigated and the pseudo-first-order kinetic model was found to be superior to the pseudo-second-order kinetic model. Overall, using metal-organic frameworks to treat dye wastewater was found to be inexpensive, feasible, and efficient. Therefore, this material has future prospects in research and applications in the purification of wastewater.
Cobalt-Assisted Morphology and Assembly Control of Co-Doped ZnO Nanoparticles
Authors: Han Xianying , Wahl Sebastian , Russo Patrícia A. , Pinna Nicola
Published: April 2018

The morphology of metal oxide nanostructures influences the response of the materials in a given application. In addition to changing the composition, doping can also modify the morphology of a host nanomaterial. Herein, we determine the effect of dopant concentration, reaction temperature, and reaction time on the morphology and assembly of CoxZn1−xO nanoparticles synthesized through non-aqueous sol-gel in benzyl alcohol. With the increase of the atom % of cobalt incorporated from 0 to 15, the shape of the nanoparticles changes from near spherical, to irregular, and finally to triangular. The tendency of the particles to assemble increases in the same direction, with Co0.05Zn0.95O consisting of non-assembled particles, whereas Co0.15Zn0.85O consists of triangular nanoparticles forming spherical structures. The morphology and assembly process are also sensitive to the reaction temperature. The assembly process is found to occur during the nucleation or the early stages of particle growth. The cobalt ions promote the change in the shape during the growth stage of the nanoparticles.
Serum Hepcidin Concentration in Individuals with Sickle Cell Anemia: Basis for the Dietary Recommendation of Iron
Authors: Omena Juliana , Cople-Rodrigues Cláudia dos Santos , Cardoso Jessyca Dias do Amaral , Soares Andrea Ribeiro , Fleury Marcos Kneip , Brito Flávia dos Santos Barbosa , Koury Josely Correa , Citelli Marta
Published: April 2018

Dietary iron requirements in patients with sickle cell disease (SCD) remain unclear. SCD is a neglected hemoglobinopathy characterized by intense erythropoietic activity and anemia. Hepcidin is the hormone mainly responsible for iron homeostasis and intestinal absorption. Intense erythropoietic activity and anemia may reduce hepcidin transcription. By contrast, iron overload and inflammation may induce it. Studies on SCD have not evaluated the role of hepcidin in the presence and absence of iron overload. We aimed to compare serum hepcidin concentrations among individuals with sickle cell anemia, with or without iron overload, and those without the disease. Markers of iron metabolism and erythropoietic activity such as hepcidin, ferritin, and growth differentiation factor 15 were evaluated. Three groups participated in the study: the control group, comprised of individuals without SCD (C); those with the disease but without iron overload (SCDw); and those with the disease and iron overload (SCDio). Results showed that hepcidin concentration was higher in the SCDio > C > SCDw group. These data suggest that the dietary iron intake of the SCDio group should not be reduced as higher hepcidin concentrations may reduce the intestinal absorption of iron.
Antifungal Paper Based on a Polyborneolacrylate Coating
Authors: Xu Jiangqi , Bai Yujia , Wan Meijiao , Liu Yanhui , Tao Lei , Wang Xing
Published: April 2018

Paper documents and products are very susceptible to microbial contamination and damage. Fungi are mainly responsible for those biodeterioration processes. Traditional microbicidal strategies constitute a serious health risk even when microbes are dead. Ideal methods should not be toxic to humans and should have no adverse effects on paper, but should own a broad spectrum, good chemical stability and low cost. In this work, we utilize an advanced antimicrobial strategy of surface stereochemistry by applying a coating of a shallow layer of polyborneolacrylate (PBA), resulting in the desired antifungal performance. The PBA-coated paper is challenged with the most common air-borne fungi growing on paper, Aspergillus niger and Penicillium sp. Ten percent by weight of the coating concentration or a 19-μm infiltration of PBA is sufficient to keep the paper spotless. The PBA coating also exhibits significant inhibition of spores’ germination. After PBA coating, both physicochemical properties (paper whiteness, pH, mechanical strength) and inking performance display only slight changes, which are acceptable for general utilization. This PBA coating method is nontoxic, rapid and cost-effective, thus demonstrating great potential for applications in paper products.
Soluble Electrochromic Polymers Incorporating Benzoselenadiazole and Electron Donor Units (Carbazole or Fluorene): Synthesis and Electronic-Optical Properties
Authors: Xu Jianzhong , Ji Qi , Kong Lingqian , Du Hongmei , Ju Xiuping , Zhao Jinsheng
Published: April 2018

A series of π-conjugated polymers containing alternating benzoselenadiazole (BSe)-bi(thiophene derivative)-carbazole or benzoththiadiazole (BSe)-bi(thiophene derivative)-fluorene units were designed and synthesized. Thiophene derivatives, namely 3-hexylthiophene, 3,4-bihexyloxythiophene, and 3,4-bioctyloxythiophene, were used as the π-bridges of the polymers. The polymers were characterized in detail in terms of their thermal stabilities, cyclic voltammograms, UV-Vis absorption, spectroelectrochemistry, dynamic switching property and so forth. The alkoxy thiophene π-bridged polymers have lower onset oxidation potentials and bandgaps than that of their corresponding alkyl thiophene π-bridged polymers. The selection of the donor units between the carbazole and the fluorene units has nearly no effect on the bandgaps and colors as well as the onset oxidation potentials of the polymers. The increase in the length of the side alkyl chains on the thiophene ring caused a slight increase in the polymer bandgap, which may be caused by the space hindrance effect. The dynamic switching abilities of the polymers were obtained by the chronoabsorptometry method, and the results also suggested that the alkoxy thiophene-containing polymers (as π-bridges) have higher contrast ratios than the corresponding alkyl thiophene-containing polymers. Furthermore, the increase in the length of the side alkyl chain might have a detrimental effect on the optical contrast ratios of the polymers.
Supporting Open Access at Kent—New Staff Roles
Authors: Bass Rosalyn , Slowe Sarah
Published: April 2018

Open Access has been supported at the University of Kent from an early stage with the establishment of the Kent Academic Repository in 2007. Initially, this work was accommodated within the existing library staff structure, but the pace of change, funder requirements, and a new university plan meant that support for Open Access needed to become explicit. Therefore, a research support team was established using a matrix working system1. This article details this new structure and reflects on the benefits and challenges it brings.
Transcultural Adaptation and Psychometric Properties of Portuguese Version of the Spiritual Needs Questionnaire (SpNQ) Among HIV Positive Patients in Brazil
Authors: Valente Tânia Cristina de Oliveira , Cavalcanti Ana Paula Rodrigues , Büssing Arndt , Costa Junior Clóvis Pereira da , Motta Rogerio Neves
Published: April 2018

The Spiritual Needs Questionnaire (SpNQ), originally written in the German language, was translated and validated into 11 languages, but not Latin languages, such as Brazilian Portuguese. This study aimed to determine the psychometric properties of the SpNQ after translation and transcultural adaptation to the Portuguese language, identifying unmet spiritual needs in a sample of patients living with HIV in Brazil. This pioneering study conformed a four-factor structure of 20 items, differentiating Religious Needs (α = 0.887), Giving/Generativity Needs (α = 0.848), Inner Peace (α = 0.813) and a new item: Family Support Needs (α = 0.778). The Brazilian version of the SpNQ (SpNQ-BR) had good internal validity criteria and can be used for research of the spiritual needs for Brazilian patients. The cross-cultural adaptation and comparison with previous studies showed that the SpNQ is sensitive to the cultural characteristics of different countries.
Development of an Innovative and Sustainable Model for Integrating River Maintenance with Energy Production from Residual Biomass
Authors: Marchetti Barbara , Corvaro Francesco , Polonara Fabio , Leporini Mariella
Published: April 2018

This study aims to develop an innovative model for managing territory maintenance in which the productive function is linked with the protective one and that integrates environmental and economic development aspects, combining the energetic valorization with an effective territory maintenance program. The strong innovation consists in the creation of an agro-energy environment chain based on the maintenance of river basins and small waterways made by single farmers or associations that will use residual biomass to produce electrical and thermal bio-energy. The maintenance activities include the control of aquatic weeds, grass cutting on river banks, and tree/bush management. If left unmanaged, they can block the flow of watercourses and increase the risk of flooding. The implementation of this virtuous model to the rivers maintenance aims to provide management and conservation means based on the territory characteristics. In fact, the new model has been applied to an existing site in the Marche region (Italy) located near a river characterized by a poor state of maintenance. A real commercial system for residual biomass exploitation was chosen, and both the technical and economic feasibility of the model implementation have been demonstrated.
Automatic Clearance Anomaly Detection for Transmission Line Corridors Utilizing UAV-Borne LIDAR Data
Authors: Chen Chi , Yang Bisheng , Song Shuang , Peng Xiangyang , Huang Ronggang
Published: April 2018

Transmission line corridor (i.e., Right-of-Ways (ROW)) clearance management plays a critically important role in power line risk management and is an important task of the routine power line inspection of the grid company. The clearance anomaly detection measures the distance between the power lines and the surrounding non-power-facility objects in the corridor such as trees, and buildings, to judge whether the clearance is within the safe range. To find the clearance hazards efficiently and flexibly, this study thus proposed an automatic clearance anomaly detection method utilizing LiDAR point clouds collected by unmanned aerial vehicle (UAV). Firstly, the terrain points were filtered out using two-step adaptive terrain filter and the pylons were detected in the non-terrain points following a feature map method. After dividing the ROW point clouds into spans based on the pylon detection results, the power line point clouds were extracted according to their geometric distribution in local span point clouds slices, and were further segmented into clusters by applying conditional Euclidean clustering with linear feature constraints. Secondly, the power line point clouds segments were iteratively fitted with 3D catenary curve model that is represented by a horizontal line and a vertical catenary curve defined by a hyperbolic cosine function, resulting in a continuous mathematical model of the discretely sampled points of the power line. Finally, a piecewise clearance calculation method which converts the point-to-catenary curve distance measurements to minimal distance calculation based on differential geometry was used to calculate the distance between the power line and the non-power-facility objects in the ROW. The clearance measurements were compared with the standard safe threshold to find the clearance anomalies in the ROWs. Multiple LiDAR point clouds datasets collected by a large-scale UAV power line inspection system were used to validate the effectiveness and accuracy of the proposed method. The detected results were validated through qualitatively visual inspection, quantitatively manual measurements in raw point clouds and on-site field survey. The experiments show that the automatic clearance anomaly detection method proposed in this paper effectively detects the clearance hazards such as tree encroachment, and the clearance measurement accuracy is decimeter level for the LiDAR point clouds collected by our UAV inspection system.