bluuebunny/crossref_metadata_2025 huggingface dataset:
Clinical Application and Efficacy of Silver Drug in Ophthalmology: A Literature Review and New Formulation of EYE Drops with Drug Silver (I) Complex of Metronidazole with Improved Dosage Form
Authors: Waszczykowska Arleta , Żyro Dominik , Ochocki Justyn , Jurowski Piotr
Published: February 2021

The use of silver preparations in medicine is becoming increasingly popular. The basic aim of this evaluation was to review the literature on the clinical (in vivo) and antibacterial potential of silver preparations in ophthalmic diseases. The second goal was to summarize the results of experimental research on the use of silver preparations in ophthalmology. The third objective was to present a method for stabilizing eye drops containing silver (I) complex. Analysis of the pH stability of the silver (I) complex with metronidazole in the prepared dosage form (eye drops) was carried out. Most silver preparations are clinically used for topical application. Few experimental results indicate the usefulness of intraocular or systemic administration of silver (I) preparations as an alternative or additional therapy in infectious and angiogenic eye diseases. The development of a new formulation increases the stability of the dosage form. New forms of silver (I) products will certainly find application in the treatment of many ophthalmic diseases. One of the most important features of the silver (I) complex is its capacity to break down bacterial resistance. The new eye drops formula can significantly improve comfort of use. Due to their chemical nature, silver (I) compounds are difficult to stabilize, especially in the finished dosage form.
Challenges and Opportunities for Drug Repositioning in Fibrodysplasia Ossificans Progressiva
Authors: Ventura Francesc , Williams Eleanor , Ikeya Makoto , Bullock Alex N. , ten Dijke Peter , Goumans Marie-José , Sanchez-Duffhues Gonzalo
Published: February 2021

Fibrodysplasia ossificans progressiva (FOP) is an ultrarare congenital disease that progresses through intermittent episodes of bone formation at ectopic sites. FOP patients carry heterozygous gene point mutations in activin A receptor type I ACVR1, encoding the bone morphogenetic protein (BMP) type I serine/threonine kinase receptor ALK2, termed activin receptor-like kinase (ALK)2. The mutant ALK2 displays neofunctional responses to activin, a closely related BMP cytokine that normally inhibits regular bone formation. Moreover, the mutant ALK2 becomes hypersensitive to BMPs. Both these activities contribute to enhanced ALK2 signalling and endochondral bone formation in connective tissue. Being a receptor with an extracellular ligand-binding domain and intrinsic intracellular kinase activity, the mutant ALK2 is a druggable target. Although there is no approved cure for FOP yet, a number of clinical trials have been recently initiated, aiming to identify a safe and effective treatment for FOP. Among other targeted approaches, several repurposed drugs have shown promising results. In this review, we describe the molecular mechanisms underlying ALK2 mutation-induced aberrant signalling and ectopic bone formation. In addition, we recapitulate existing in vitro models to screen for novel compounds with a potential application in FOP. We summarize existing therapeutic alternatives and focus on repositioned drugs in FOP, at preclinical and clinical stages.
Modulation of Interhemispheric Inhibition between Primary Motor Cortices Induced by Manual Motor Imitation: A Transcranial Magnetic Stimulation Study
Authors: Tian Dongting , Izumi Shin-ichi , Suzuki Eizaburo
Published: February 2021

Imitation has been proven effective in motor development and neurorehabilitation. However, the relationship between imitation and interhemispheric inhibition (IHI) remains unclear. Transcranial magnetic stimulation (TMS) can be used to investigate IHI. In this study, the modification effects of IHI resulting from mirror neuron system (MNS) activation during different imitations are addressed. We measured IHI between homologous primary motor cortex (M1) by analyzing the ipsilateral silent period (iSP) evoked by single-pulse focal TMS during imitation and analyzed the respective IHI modulation during and after different patterns of imitation. Our main results showed that throughout anatomical imitation, significant time-course changes of iSP duration through the experiment were observed in both directions. iSP duration declined from the pre-imitation time point to the post-imitation time point and did not return to baseline after 30 min rest. We also observed significant iSP reduction from the right hemisphere to the left hemisphere during anatomical and specular imitation, compared with non-imitative movement. Our findings indicate that using anatomical imitation in action observation and execution therapy promotes functional recovery in neurorehabilitation by regulating IHI.
Removal of Lead by Oxidized Graphite
Authors: Selvanantharajah Namasivayam , Iyngaran Poobalasuntharam , Abiman Poobalasingam , Kuganathan Navaratnarajah
Published: February 2021

Lead ion adsorption on the surfaces of pristine and oxidized graphite is studied quantitatively using X-ray photoelectron spectroscopy (XPS) and standard electrochemical measurements. The XPS analysis confirmed the oxidation of graphite, yielding a final composite consisting of 15.97% of oxygen and 84.03% of carbon in comparison with the pristine graphite powder consisting of 6.13% oxygen and 93.87% carbon. The adsorption of lead (II) ion was confirmed by the peaks observed at 138 eV and 143.8 eV, associated with the emissions from Pb4f 7/2 and Pb4f 5/2, respectively. The effective concentration of Pb2+ ion and the optimum dosage of oxidized graphite were calculated to be 400 µM and 200 mg, respectively. Adsorption capacity of bare graphite was 41.18%, whereas that of oxidized graphite was 73.3%. The present results show that graphite oxide is a candidate material for the adsorption of Pb2+ ion from water.
Autophagy and Mitophagy as Essential Components of Atherosclerosis
Authors: Poznyak Anastasia V. , Nikiforov Nikita G. , Wu Wei-Kai , Kirichenko Tatiana V. , Orekhov Alexander N.
Published: February 2021

Cardiovascular disease (CVD) is one of the greatest health problems affecting people worldwide. Atherosclerosis, in turn, is one of the most common causes of cardiovascular disease. Due to the high mortality rate from cardiovascular diseases, prevention and treatment at the earliest stages become especially important. This requires developing a deep understanding of the mechanisms underlying the development of atherosclerosis. It is well-known that atherogenesis is a complex multi-component process that includes lipid metabolism disorders, inflammation, oxidative stress, autophagy disorders and mitochondrial dysfunction. Autophagy is a cellular control mechanism that is critical to maintaining health and survival. One of the specific forms of autophagy is mitophagy, which aims to control and remove defective mitochondria from the cell. Particularly defective mitophagy has been shown to be associated with atherogenesis. In this review, we consider the role of autophagy, focusing on a special type of it—mitophagy—in the context of its role in the development of atherosclerosis.
Development of BCR-ABL1 Transgenic Zebrafish Model Reproducing Chronic Myeloid Leukemia (CML) Like-Disease and Providing a New Insight into CML Mechanisms
Authors: Zizioli Daniela , Bernardi Simona , Varinelli Marco , Farina Mirko , Mignani Luca , Bosio Katia , Finazzi Dario , Monti Eugenio , Polverelli Nicola , Malagola Michele , Borsani Elisa , Borsani Giuseppe , Russo Domenico
Published: February 2021

Zebrafish has proven to be a versatile and reliable experimental in vivo tool to study human hematopoiesis and model hematological malignancies. Transgenic technologies enable the generation of specific leukemia types by the expression of human oncogenes under specific promoters. Using this technology, a variety of myeloid and lymphoid malignancies zebrafish models have been described. Chronic myeloid leukemia (CML) is a clonal myeloproliferative neoplasia characterized by the BCR-ABL1 fusion gene, derived from the t (9;22) translocation causing the Philadelphia Chromosome (Ph). The BCR-ABL1 protein is a constitutively activated tyrosine kinas inducing the leukemogenesis and resulting in an accumulation of immature leukemic cells into bone marrow and peripheral blood. To model Ph+ CML, a transgenic zebrafish line expressing the human BCR-ABL1 was generated by the Gal4/UAS system, and then crossed with the hsp70-Gal4 transgenic line. The new line named (BCR-ABL1pUAS:CFP/hsp70-Gal4), presented altered expression of hematopoietic markers during embryonic development compared to controls and transgenic larvae showed proliferating hematopoietic cells in the caudal hematopoietic tissue (CHT). The present transgenic zebrafish would be a robust CML model and a high-throughput drug screening tool.
Choline Glycerophospholipid-Derived Prostaglandins Attenuate TNFα Gene Expression in Macrophages via a cPLA2α/COX-1 Pathway
Authors: Astudillo Alma M. , Rodríguez Juan P. , Guijas Carlos , Rubio Julio M. , Balboa María A. , Balsinde Jesús
Published: February 2021

Macrophages are professional antigen presenting cells with intense phagocytic activity, strategically distributed in tissues and cavities. These cells are capable of responding to a wide variety of innate inflammatory stimuli, many of which are signaled by lipid mediators. The distribution of arachidonic acid (AA) among glycerophospholipids and its subsequent release and conversion into eicosanoids in response to inflammatory stimuli such as zymosan, constitutes one of the most studied models. In this work, we used liquid and/or gas chromatography coupled to mass spectrometry to study the changes in the levels of membrane glycerophospholipids of mouse peritoneal macrophages and the implication of group IVA cytosolic phospholipase A2 (cPLA2α) in the process. In the experimental model used, we observed that the acute response of macrophages to zymosan stimulation involves solely the cyclooxygenase-1 (COX-1), which mediates the rapid synthesis of prostaglandins E2 and I2. Using pharmacological inhibition and antisense inhibition approaches, we established that cPLA2α is the enzyme responsible for AA mobilization. Zymosan stimulation strongly induced the hydrolysis of AA-containing choline glycerophospholipids (PC) and a unique phosphatidylinositol (PI) species, while the ethanolamine-containing glycerophospholipids remained constant or slightly increased. Double-labeling experiments with 3H- and 14C-labeled arachidonate unambiguously demonstrated that PC is the major, if not the exclusive source, of AA for prostaglandin E2 production, while both PC and PI appeared to contribute to prostaglandin I2 synthesis. Importantly, in this work we also show that the COX-1-derived prostaglandins produced during the early steps of macrophage activation restrict tumor necrosis factor-α production. Collectively, these findings suggest new approaches and targets to the selective inhibition of lipid mediator production in response to fungal infection.
Insights into Solution Structures of Photosynthetic Protein Complexes from Small-Angle Scattering Methods
Authors: Golub Maksym , Kölsch Adrian , Feoktystov Artem , Zouni Athina , Pieper Jörg
Published: February 2021

High-resolution structures of photosynthetic pigment–protein complexes are often determined using crystallography or cryo-electron microscopy (cryo-EM), which are restricted to the use of protein crystals or to low temperatures, respectively. However, functional studies and biotechnological applications of photosystems necessitate the use of proteins isolated in aqueous solution, so that the relevance of high-resolution structures has to be independently verified. In this regard, small-angle neutron and X-ray scattering (SANS and SAXS, respectively) can serve as the missing link because of their capability to provide structural information for proteins in aqueous solution at physiological temperatures. In the present review, we discuss the principles and prototypical applications of SANS and SAXS using the photosynthetic pigment–protein complexes phycocyanin (PC) and Photosystem I (PSI) as model systems for a water-soluble and for a membrane protein, respectively. For example, the solution structure of PSI was studied using SAXS and SANS with contrast matching. A Guinier analysis reveals that PSI in solution is virtually free of aggregation and characterized by a radius of gyration of about 75 Å. The latter value is about 10% larger than expected from the crystal structure. This is corroborated by an ab initio structure reconstitution, which also shows a slight expansion of Photosystem I in buffer solution at room temperature. In part, this may be due to conformational states accessible by thermally activated protein dynamics in solution at physiological temperatures. The size of the detergent belt is derived by comparison with SANS measurements without detergent match, revealing a monolayer of detergent molecules under proper solubilization conditions.
Evolution Mechanism of Transient Strain and Residual Stress Distribution in Al 6061 Laser Welding
Authors: Rong Youmin , Huang Yu , Wang Lu
Published: February 2021

Considering the harm that residual stress causes to the mechanical properties of a weld joint, the evolution mechanisms of transient strain and residual stress distribution are investigated in laser welding of Al 6061, considering that these originate from non-uniform temperature distribution and are intensified further by the unbalanced procedure of melting and solidification. Thermal-elastic-plastic finite element method is developed and analyzed, while the actual weld profile is novel fitted by a B-spline curve. Transient strain is extracted by strain gauges. Longitudinal strain starts from a fluctuating compressive state and progresses to an ultimate residual tension state at the starting and ending welding positions, respectively. The maximum fitting deviation of the weld profile is 0.13 mm. Experimental and simulation results of residual strain are 842.0 μ and 826.8 μ, with a relative error of 1.805% at the starting position and −17.986% at the ending position. Near the weld center, mechanical behavior is complexly influenced by thermal expansion and contraction in the weld zone and the reaction binding force of the solid metal. Within a distance between −10 mm and 10 mm, and longitudinal stress is in a tension state, transverse stress fluctuates with a high gradient (~100 MPa).
Extending Libraries of Extremely Localized Molecular Orbitals to Metal Organic Frameworks: A Preliminary Investigation
Authors: Wieduwilt Erna , Macetti Giovanni , Scatena Rebecca , Macchi Piero , Genoni Alessandro
Published: February 2021

Libraries of extremely localized molecular orbitals (ELMOs) have been recently assembled to reconstruct approximate wavefunctions of very large biological systems, such as polypeptides and proteins. In this paper, we investigate for the first time the possibility of using ELMO transferability to also quickly obtain wavefunctions, electron densities, and electrostatic potentials of three-dimensional coordination polymers such as metal organic frameworks (MOFs). To accomplish this task, we propose a protocol that, in addition to exploiting the usual exportability of extremely localized molecular orbitals, also takes advantage of the novel QM/ELMO (quantum mechanics/extremely localized molecular orbital) approach to properly describe the secondary building units of MOFs. As a benchmark test, our technique has been applied to the well-known metal organic framework HKUST-1 ({Cu3(BTC)2}n, with BTC=1,3,5-benzenetricarboxylate) to quickly calculate electrostatic potential maps in the small and large cavities inside the network. On the basis of the obtained results, we envisage further improvements and applications of this strategy, which can be also seen as a starting point to perform less computationally expensive quantum mechanical calculations on metal organic frameworks with the goal of investigating transformation phenomena such as chemisorption.
Characterisation and Life Cycle Assessment of Pervious Concrete with Recycled Concrete Aggregates
Authors: Paula Junior Adilson , Jacinto Cláudia , Oliveira Thaís , Polisseni Antonio , Brum Fabio , Teixeira Elisabete , Mateus Ricardo
Published: February 2021

The search for environmental preservation and conservation of natural resources gives rise to new concepts and viable technical solutions on the path to sustainable development. In this context, this study’s main objective is to analyse the influence of recycled concrete aggregates (RCAs) on the development of pervious concrete, whose use as a floor covering represents an excellent device to mitigate the urban soil sealing phenomena. For this, mechanical and hydraulic tests were carried out, in addition to microstructural analyses and the assessment of its environmental performance. The results obtained were compared to reference studies also involving the incorporation of recycled aggregates. A pilot-scale case study was conducted, involving a parking space lined with pervious concrete moulded “in situ”. In laboratory tests, permeability coefficients and mechanical strengths compatible with the literature and above the normative limit for light traffic were found. The case study demonstrated higher permeability than in the laboratory, but the flexural strength was lower, being indicated only for pedestrian traffic. The environmental assessment showed that the RCA represents a positive contribution to the environmental performance of pervious concrete. Still, attention should be given to the recycled aggregate transport distance between the concrete plant and the RCA treatment plant.
Light-Emitting Diodes (LED): A Promising Street Light System to Reduce the Attraction to Light of Insects
Authors: Martín Beatriz , Pérez Héctor , Ferrer Miguel
Published: February 2021

Currently, there is a demand for more energy-efficient lighting sources, however, light emitted by different lighting systems differs in primary properties such as intensity, propagation direction, and wavelength spectrum, among others, and these properties may affect insect light attraction. Despite the energetic benefits of light-emitting diodes (LED) as street light systems, their ecological impacts on insects have not yet been tested on a wide range of taxa. Using an experimental approach, we showed that LED street lights lead to a reduction in the total number of insects captured with light traps in a wide range of families. Coleoptera and Lepidoptera orders were the most sensitive groups to ecological light pollution in the study area. We suggest that LED was the least attractive light system for most of the affected groups both because of its very little emitted short-wavelength light and because of its lower light intensity. We expect that the more and more widespread use of LED lights as a measure to reduce economic costs of outdoor lighting should lead to a lower attraction to street lights in most of the affected insect taxa and to diminish the negative impacts of artificial light attraction on the ecosystems.
Fast and Automated Segmentation for the Three-Directional Multi-Slice Cine Myocardial Velocity Mapping
Authors: Wu Yinzhe , Hatipoglu Suzan , Alonso-Álvarez Diego , Gatehouse Peter , Li Binghuan , Gao Yikai , Firmin David , Keegan Jennifer , Yang Guang
Published: February 2021

Three-directional cine multi-slice left ventricular myocardial velocity mapping (3Dir MVM) is a cardiac magnetic resonance (CMR) technique that allows the assessment of cardiac motion in three orthogonal directions. Accurate and reproducible delineation of the myocardium is crucial for accurate analysis of peak systolic and diastolic myocardial velocities. In addition to the conventionally available magnitude CMR data, 3Dir MVM also provides three orthogonal phase velocity mapping datasets, which are used to generate velocity maps. These velocity maps may also be used to facilitate and improve the myocardial delineation. Based on the success of deep learning in medical image processing, we propose a novel fast and automated framework that improves the standard U-Net-based methods on these CMR multi-channel data (magnitude and phase velocity mapping) by cross-channel fusion with an attention module and the shape information-based post-processing to achieve accurate delineation of both epicardial and endocardial contours. To evaluate the results, we employ the widely used Dice Scores and the quantification of myocardial longitudinal peak velocities. Our proposed network trained with multi-channel data shows superior performance compared to standard U-Net-based networks trained on single-channel data. The obtained results are promising and provide compelling evidence for the design and application of our multi-channel image analysis of the 3Dir MVM CMR data.
Females and Males Show Differences in Early-Stage Transcriptomic Biomarkers of Lung Adenocarcinoma and Lung Squamous Cell Carcinoma
Authors: Liu Quewang , Wang Yueying , Duan Meiyu , Fan Yusi , Pan Xingyuan , Liu Shuai , Yu Qiong , Huang Lan , Zhou Fengfeng
Published: February 2021

The incidence and mortality rates of lung cancers are different between females and males. Therefore, sex information should be an important part of how to train and optimize a diagnostic model. However, most of the existing studies do not fully utilize this information. This study carried out a comparative investigation between sex-specific models and sex-independent models. Three feature selection algorithms and five classifiers were utilized to evaluate the contribution of the sex information to the detection of early-stage lung cancers. Both lung adenocarcinoma (LUAD) and lung squamous cell carcinoma (LUSC) showed that the sex-specific models outperformed the sex-independent detection of early-stage lung cancers. The Venn plots suggested that females and males shared only a few transcriptomic biomarkers of early-stage lung cancers. Our experimental data suggested that sex information should be included in optimizing disease diagnosis models.
Evaluation of MicroScan Bacterial Identification Panels for Low-Resource Settings
Authors: Ombelet Sien , Natale Alessandra , Ronat Jean-Baptiste , Vandenberg Olivier , Hardy Liselotte , Jacobs Jan
Published: February 2021

Bacterial identification is challenging in low-resource settings (LRS). We evaluated the MicroScan identification panels (Beckman Coulter, Brea, CA, USA) as part of Médecins Sans Frontières’ Mini-lab Project. The MicroScan Dried Overnight Positive ID Type 3 (PID3) panels for Gram-positive organisms and Dried Overnight Negative ID Type 2 (NID2) panels for Gram-negative organisms were assessed with 367 clinical isolates from LRS. Robustness was studied by inoculating Gram-negative species on the Gram-positive panel and vice versa. The ease of use of the panels and readability of the instructions for use (IFU) were evaluated. Of species represented in the MicroScan database, 94.6% (185/195) of Gram-negative and 85.9% (110/128) of Gram-positive isolates were correctly identified up to species level. Of species not represented in the database (e.g., Streptococcus suis and Bacillus spp.), 53.1% out of 49 isolates were incorrectly identified as non-related bacterial species. Testing of Gram-positive isolates on Gram-negative panels and vice versa (n = 144) resulted in incorrect identifications for 38.2% of tested isolates. The readability level of the IFU was considered too high for LRS. Inoculation of the panels was favorably evaluated, whereas the visual reading of the panels was considered error-prone. In conclusion, the accuracy of the MicroScan identification panels was excellent for Gram-negative species and good for Gram-positive species. Improvements in stability, robustness, and ease of use have been identified to assure adaptation to LRS constraints.
Initial Experience with 64Cu-DOTATATE Digital PET of Patients with Neuroendocrine Neoplasms: Comparison with Analog PET
Authors: Loft Mathias , Johnbeck Camilla B. , Carlsen Esben A. , Johannesen Helle H. , Oturai Peter , Langer Seppo W. , Knigge Ulrich , Kjaer Andreas
Published: February 2021

The recent introduction of solid-state detectors in clinical positron emission tomography (PET) scanners has significantly improved image quality and spatial resolution and shortened acquisition time compared to conventional analog PET scanners. In an initial evaluation of the performance of our newly acquired Siemens Biograph Vision 600 PET/CT (digital PET/CT) scanner for 64Cu-DOTATATE imaging, we compared PET/CT acquisitions from patients with neuroendocrine neoplasms (NENs) grades 1 and 2 and stable disease on CT who were scanned on both our Siemens Biograph 128 mCT PET/CT (analog PET/CT) and digital PET/CT within 6 months as part of their routine clinical management. Five patients fulfilled the criteria and were included in the analysis. The digital PET acquisition time was less than 1/3 of the analog PET acquisition time (digital PET, mean (min:s): 08:20 (range, 07:59–09:45); analog PET, 25:28 (24:39–28:44), p < 0.001). All 44 lesions detected on the analog PET with corresponding structural correlates on the CT were also found on the digital PET performed 137 (107–176) days later. Our initial findings suggest that digital 64Cu-DOTATATE PET can successfully be performed in patients with NENs using an image acquisition time of only 1/3 of what is used for an analog 64Cu-DOTATATE PET.
Paravertebral Muscle Mechanical Properties and Spinal Range of Motion in Patients with Acute Neck or Low Back Pain: A Case-Control Study
Authors: Alcaraz-Clariana Sandra , García-Luque Lourdes , Garrido-Castro Juan Luis , Fernández-de-las-Peñas César , Carmona-Pérez Cristina , Rodrigues-de-Souza Daiana Priscila , Alburquerque-Sendín Francisco
Published: February 2021

Our aims were to identify potential differences in muscle mechanical properties (MMPs) of cervical and lumbar tissues and in spinal range of motion (ROM) between patients with acute low back pain (LBP) or acute neck pain (NP) and healthy controls, and to identify if ROMs and MMPs are able to identify subjects among the three groups. Clinical variables (pain, disability, fear of movement, kinesiophobia, quality of life), MMPs and ROMs were obtained in 33 subjects with acute LBP, 33 with acute NP, and 33 healthy control subjects. Between-groups differences and explanatory models to discriminate groups depending on MMPs and ROMs were calculated. The results showed that cervical tone was higher in patients with acute NP than in controls, while cervical decrement was higher in both spinal pain groups. Patients with acute NP showed reduced cervical flexion when compared to acute LBP and control groups, and also cervical rotation, but just against controls. Furthermore, lumbar flexion was reduced in patients with acute LBP when compared to those with acute NP. Cervical decrement was able to discriminate spinal pain individuals from controls in a multinominal regression (R2: Cox–Snell estimation = 0.533; Nagelkerke estimation = 0.600). Lumbar flexion differentiated patients with acute LBP and controls, whereas cervical flexion differentiated patients with acute NP and controls. This study supports a tendency of the affectation of other spinal regions when only one is affected.
A Simplified Output Feedback Controller for the DC-DC Boost Power Converter
Authors: Chincholkar Satyajit , Jiang Wentao , Chan Chok-You , S. Rangarajan Shriram
Published: February 2021

Boost-type dc-dc converters present non-minimum phase dynamic system characteristics. Therefore, controller design using only the output voltage for feedback purposes is not a very straightforward task. Even though output voltage control can be achieved using inductor current control, the implementation of such current-mode controllers may require prior knowledge of the load resistance and also demand more states such as one or more currents in feedback. In this paper, the development of a new output feedback controller for boost-type dc-dc converters is presented. The controller form is such that it avoids the possibility of saturation in the control signal due to division by zero. The basic structure of the proposed controller is firstly obtained from the expression of the open-loop control signal, and the complete controller structure is then derived to satisfy the closed-loop stability conditions. Simulation and experimental results clearly verify the ability of the control law to provide robust regulation against parameter variations.
Transient System Model for the Analysis of Structural Dynamic Interactions of Electric Drivetrains
Authors: Franck Marius , Rickwärtz Jan Philipp , Butterweck Daniel , Nell Martin , Hameyer Kay
Published: February 2021

In electric drivetrains, the traction machines are often coupled to a gear transmission. For the noise and vibration analysis of such systems, linearised system models in the frequency domain are commonly used. In this paper, a system approach in the time domain is introduced, which gives the advantage of analysing the transient behaviour of an electric drivetrain. The focus in this paper is on the dynamic gear model. Finally, the modelling approach is applied to an exemplary drivetrain, and the results are discussed.
Effect of Soil Management and Training System on Negroamaro Wine Aroma
Authors: Coletta Antonio , Toci Aline Theodoro , Pati Sandra , Ferrara Giuseppe , Grieco Francesco , Tufariello Maria , Crupi Pasquale
Published: February 2021

This study aimed to assess the impact of two soil managements and training systems on yield and wine aroma compounds of Negroamaro variety grown in a warm climate region (southern Italy). Cover crop (CC) and soil tillage (ST) as soil management, whilst bilateral Guyot (BG) and monolateral Guyot (MG) as training systems were compared. Free and bound volatile fractions were evaluated by GC-MS. ST and CC as well as BG and MG significantly affected yield parameters. In particular, yield was higher in ST and BG than in CC and MG, respectively; moreover, it was found to be positively influenced by interaction between BG and ST. Regarding aroma compounds, significant interactions between soil management and training system factors were observed. In case of free volatiles, the most positive interaction was found between BG and ST, whereas, for bound volatiles, the best interaction was represented by MG with both soil tillage and cover crop. Vine leaf area and development over vine growth stages along with water stress levels played an important role in determining the aroma profile as well as yield parameters. In conclusion, the training system significantly interacted with soil management and affected most of important aroma compounds in Negroamaro wine.
Artichoke by Products as a Source of Antioxidant and Fiber: How It Can Be Affected by Drying Temperature
Authors: Borsini Ariel A. , Llavata Beatriz , Umaña Mónica , Cárcel Juan A.
Published: February 2021

The reuse of food industry by-products constitutes one of the essential pillars of the change from a linear to a circular economic model. Drying is one of the most affordable techniques with which to stabilize by-products, making their subsequent processing possible. However, it can affect material properties. The objective of this study was to assess the effect of the drying temperature on the drying kinetics and final quality of the main artichoke processing by-products, bracts and stems, which have never been studied as independent materials. For this purpose, air drying experiments at different temperatures (40, 60, 80, 100 and 120 °C) were carried out. The alcohol insoluble residue (AIR) and the total phenolic content (TPC), antioxidant capacity (AC) and vitamin C (VC) of the fresh and dried samples were determined. The bracts dried faster than the stems, increasing drying rate with temperature. The two by-products presented relatively large amounts of AIR, the content being higher in bracts, but better functional properties in stems. The TPC, AC and VC values of the dried samples decreased in relation to the fresh samples, with the temperatures of 40 °C (bracts) and 120 °C (stems) being the most adequate for the purposes of preserving these characteristics.
Fabrication and Evaluation of Silk Sericin-Derived Hydrogel for the Release of the Model Drug Berberine
Authors: Yan Chi , Liang Jianwei , Fang Hao , Meng Xizhi , Chen Jiale , Zhong Zhi , Liu Qin , Hu Hongmei , Zhang Xiaoning
Published: February 2021

Silk sericin (SS) produced by Bombyx mori is normally discarded as waste in manufacturing processes, which causes environmental pollution. Therefore, investigating the use of silk sericin has economic and environmental benefits. As a three-dimensional structure, the sericin-derived hydrogel was explored in different applications. However, many developed gelation procedures raise concerns regarding safety, cost, and duration of gelation time. In this work, “thiol-ene” click chemistry was used to quickly and controllably prepare an SS-derived hydrogel to resolve these early concerns. Then, berberine was loaded and used as a model for investigating the drug-release profiles of the prepared hydrogel. The experimental results revealed that this hydrogel is eligible for a long-term release of berberine. Throughout the antibacterial experiments, the released berberine maintained its antibacterial activity. Our work expands the application of SS in biomedical industries in an eco-friendly way. Furthermore, the discussed strategy could provide a reference for the subsequent development of SS-derived materials.
Detection of Somatic Mutations with ddPCR from Liquid Biopsy of Colorectal Cancer Patients
Authors: Zmrzljak Uršula Prosenc , Košir Rok , Krivokapić Zoran , Radojković Dragica , Nikolić Aleksandra
Published: February 2021

Liquid biopsy and cell-free DNA (cfDNA) show great promise in cancer diagnostics. In this study, we designed a custom droplet digital PCR (ddPCR) assay for the quantification and quality control of cfDNA isolated from serum. The assay was validated on a group of locally advanced colorectal cancer (CRC) patients and two control groups—patients with hemorrhoids and healthy individuals. The assay shows a high correlation with Qubit measurement (r = 0.976) but offers a higher dynamic range. Mean concentrations of cfDNA were 12.36 ng/µL, 5.17 ng/µL, and 0.29 ng/µL for CRC, hemorrhoid patients, and healthy controls, respectively. The quality of cfDNA was assessed with the measurement of B-cell DNA contamination. On a subset of CRC patients, we compared the mutation status on KRAS (G12A, G12D, G12V, G13D) and BRAF (V600E) genes in the primary tumor and cfDNA isolated from the serum. A total of 70.6% of primary tumor samples were mutated, and the mean fractional abundance of mutations was 9.50%. The matching serum samples were mutated in 38% cases with an average fractional abundance of 0.23%. We conclude that any decisions based solely on the amount of cfDNA present in patient serum must be interpreted carefully and in the context of co-morbidities. This study explores the potential of ddPCR somatic mutations detection from liquid biopsy as a supplement to tissue biopsy in targeted personalized CRC patient management.
Development of a 76k Alpaca (Vicugna pacos) Single Nucleotide Polymorphisms (SNPs) Microarray
Authors: Calderon Marcos , More Manuel J. , Gutierrez Gustavo A. , Ponce de León Federico Abel
Published: February 2021

Small farm producers’ sustenance depends on their alpaca herds and the production of fiber. Genetic improvement of fiber characteristics would increase their economic benefits and quality of life. The incorporation of molecular marker technology could overcome current limitations for the implementation of genetic improvement programs. Hence, the aim of this project was the generation of an alpaca single nucleotide polymorphism (SNP) microarray. A sample of 150 Huacaya alpacas from four farms, two each in Puno and Cerro de Pasco were used for SNP discovery by genotyping by sequencing (GBS). Reduced representation libraries, two per animal, were produced after DNA digestion with ApeK1 and double digestion with Pst1-Msp1. Ten alpaca genomes, sequenced at depths between 12× to 30×, and the VicPac3.1 reference genome were used for read alignments. Bioinformatics analysis discovered 76,508 SNPs included in the microarray. Candidate genes SNPs (302) for fiber quality and color are also included. The microarray SNPs cover 90.5% of the genome length with a density of about 39 ± 2.51 SNPs/Mb of DNA at an average interval of 26.45 ± 18.57 kbp. The performance was evaluated by genotyping 30 family trios and comparing them to their pedigrees, as well as comparing microarray to GBS genotypes. Concordance values of 0.93 and 0.94 for ApeK1 and Pst1-Msp1 generated SNPs were observed. Similarly, 290 fiber quality and color candidate gene SNPs were validated. Availability of this microarray will facilitate genome-wide association studies, marker-assisted selection and, in time, genomic selection.
Post-Impact Faulting of the Holfontein Granophyre Dike of the Vredefort Impact Structure, South Africa, Inferred from Remote Sensing, Geophysics, and Geochemistry
Authors: Clark Martin D. , Kovaleva Elizaveta , Huber Matthew S. , Fourie Francois , Harris Chris
Published: February 2021

Better characterization features borne from long-term crustal modification processes is essential for understanding the dynamics of large basin-forming impact structures on Earth. Within the deeply eroded 2.02 Ga Vredefort Impact Structure in South Africa, impact melt dikes are exposed at the surface. In this study, we utilized a combination of field, remote sensing, electrical resistivity, magnetic, petrographical, and geochemical techniques to characterize one such impact melt dike, namely, the Holfontein Granophyre Dike (HGD), along with the host granites. The HGD is split into two seemingly disconnected segments. Geophysical modeling of both segments suggests that the melt rock does not penetrate below the modern surface deeper than 5 m, which was confirmed by a later transecting construction trench. Even though the textures and clast content are different in two segments, the major element, trace element, and O isotope compositions of each segment are indistinguishable. Structural measurements of the tectonic foliations in the granites, as well as the spatial expression of the dike, suggest that the dike was segmented by an ENE–WSW trending sinistral strike-slip fault zone. Such an offset must have occurred after the dike solidified. However, the Vredefort structure has not been affected by any major tectonic events after the impact occurred. Therefore, the inferred segmentation of the HGD is consistent with long-term crustal processes occurring in the post-impact environment. These crustal processes may have involved progressive uplift of the crater floor, which is consistent with post-impact long-term crustal adjustment that has been inferred for craters on the Moon.
Enhanced Oil Recovery Using CO2 in Alaska
Authors: Dogah Banabas , Atashbari Vahid , Ahmadi Mohabbat , Sheets Brent
Published: February 2021

Alaska holds more than 68 billion barrels of proved oil reserves and more than 36.7 trillion cubic feet of proved natural gas reserves with some special conditions such as proximity to permafrost, making Alaskan petroleum reserves unique. The low temperature in shallow reservoirs prohibited hydrocarbons’ ideal maturation, thereby generating several heavy and viscous oil accumulations in this state. This also limits the enhanced oil recovery (EOR) options, leaving the thermal methods off the table to avoid permafrost thawing, which can cause wellbore collapse. Several solutions have been attempted for improving oil production from heavy and viscous oil in Alaska; however, they have not yielded the desired recovery, and ultimate recovery factors are still less than the global average. One solution identified as a better alternative is using CO2 as an injecting fluid, alternated by water or mixed with other injectants. This paper provides a comprehensive overview of all studies on using CO2 for enhanced oil recovery purposes in Alaska and highlights common and unique challenges this approach may face. The suitability of CO2-EOR methods in the Alaskan oil pools is examined, and a ranking of the oil pools with publicly available data is provided.
Prevalence and Associated Factors of Polypharmacy in Nursing Home Residents: A Cross-Sectional Study
Authors: Cadenas Raquel , Diez María José , Fernández Nélida , García Juan José , Sahagún Ana M. , Sierra Matilde , López Cristina , Susperregui Julen , Díez Raquel
Published: February 2021

In Spain, there has been a progressive increase in aging. Specifically, Leon has been one of the Spanish provinces with the highest aging index. Polypharmacy is highly prevalent among the elderly, with significant consequences for safety. The aim of this study was to assess the consumption of drugs in a nursing home in the province of Leon and establish the relationship between polypharmacy and the most common comorbid diseases. A descriptive, observational, and cross-sectional study design was used. Residents’ information and treatments were collected by reviewing on medical charts, completed with clinical information obtained from the physician of the nursing home. The mean age of residents was 86.8 years, and 63.8% were female. Mean medical consumption amounted to 7.02 prescriptions. Polypharmacy was observed in 54.9% of residents, and excessive polypharmacy in 22.1%. The most commonly used medications (ATC classification) were those acting on the cardiovascular system (84.4%) and the nervous system (82.8%). A high-level of drug consumption was observed in the nursing home. Interventions should focus on those residents hospitalized the last year and with recent contact with a general practitioner. There is a need to develop a comprehensive monitoring system to assess the quality of prescriptions for nursing home residents.
Research Trends of Biodegradation of Cooking Oil in Antarctica from 2001 to 2021: A Bibliometric Analysis Based on the Scopus Database
Authors: Zahri Khadijah Nabilah Mohd , Zulkharnain Azham , Sabri Suriana , Gomez-Fuentes Claudio , Ahmad Siti Aqlima
Published: February 2021

In the present age, environmental pollution is multiplying due to various anthropogenic activities. Pollution from waste cooking oil is one of the main issues facing the current human population. Scientists and researchers are seriously concerned about the oils released from various activities, including the blockage of the urban drainage system and odor issues. In addition, cooking oil is known to be harmful and may have a carcinogenic effect. It was found that current research studies and publications are growing on these topics due to environmental problems. A bibliometric analysis of studies published from 2001 to 2021 on cooking oil degradation was carried out using the Scopus database. Primarily, this analysis identified the reliability of the topic for the present-day and explored the past and present progresses of publications on various aspects, including the contributing countries, journals and keywords co-occurrence. The links and interactions between the selected subjects (journals and keywords) were further visualised using the VOSviewer software. The analysis showed that the productivity of the publications is still developing, with the most contributing country being the United States, followed by China and India with 635, 359 and 320 publications, respectively. From a total of 1915 publications, 85 publications were published in the Journal of Agricultural and Food Chemistry. Meanwhile, the second and third of the most influential journals were Bioresource Technology and Industrial Crops and Products with 76 and 70 total publications, respectively. Most importantly, the co-occurrence of the author’s keywords revealed “biodegradation”, “bioremediation”, “vegetable oil” and “Antarctic” as the popular topics in this study area, especially from 2011 to 2015. In conclusion, this bibliometric analysis on the degradation of cooking oil may serve as guide for future avenues of research in this area of research.
“We All Really Need to just Take a Breath”: Composite Narratives of Hospital Doctors’ Well-Being during the COVID-19 Pandemic
Authors: Creese Jennifer , Byrne John-Paul , Conway Edel , Barrett Elizabeth , Prihodova Lucia , Humphries Niamh
Published: February 2021

The coronavirus disease 2019 (COVID-19) pandemic poses a challenge to the physical and mental well-being of doctors worldwide. Countries around the world introduced severe social restrictions, and significant changes to health service provision in the first wave of the pandemic to suppress the spread of the virus and prioritize healthcare for those who contracted it. This study interviewed 48 hospital doctors who worked in Ireland during the first wave of the pandemic and investigated their conceptualizations of their own well-being during that time (March–May 2020). Doctors were interviewed via Zoom™ or telephone. Interview transcripts were analyzed using structured thematic analysis. Five composite narratives are presented which have been crafted to illustrate themes and experiences emerging from the data. This study found that despite the risks of contracting COVID-19, many doctors saw some improvements to their physical well-being in the first wave of the pandemic. However, most also experienced a decline in their mental well-being due to anxiety, emotional exhaustion, guilt, isolation and poor support. These findings shed light on doctor well-being during COVID-19, and the ways in which they have been affected by the pandemic, both professionally and personally. The paper concludes by highlighting how doctors’ work life and well-being can be better supported during and after the COVID-19 pandemic.
A Training Proposal to Improve Multidirectional Running Technique in Male and Female Handball Players: A Pilot Study
Authors: Ferragut Carmen , Pedreira Román , Espina José Julio , Vila Helena
Published: February 2021

Multidirectional running has been described as an important factor in team sports performance. The aim of the present study was to determine changes in T-test, 505 time, 10 m sprint, squat jump (SJ), countermovement jump (CMJ), countermovement jump right leg (CMJRL), and countermovement jump left leg (CMJLL) following exposure to 12 sessions over 4 weeks of a multidirectional running sprint training intervention in male and female handball players. A total of 31 handball players (15 male and 16 female) were recruited for this study and then randomly assigned to an experimental group (EG) or control group (CG). Male EG players showed improvements in 505 Preferred Side (PS) (p ≤ 0.05), 505 Non-Preferred Side (NPS) (p ≤ 0.05), and 10 m sprint (p ≤ 0.05), while female EG players presented statistically significant improvements between pre- and post-test for the T-test (p ≤ 0.05), 505 PS (p ≤ 0.05), 505 NPS (p ≤ 0.05), and 10 m sprint (p ≤ 0.05). No statistically significant pre- and post-test differences were observed in CG (all p ≥ 0.05) or between male and female players. We found an improvement in handball players’ agility and speed of movement following the intervention protocol, suggesting the need to introduce this program into our training sessions. It may also be necessary to select and develop more specific tests in order to evaluate multidirectional work in handball players.