bluuebunny/crossref_metadata_2025 huggingface dataset:
Brain Protection from Stroke with Intravenous TNFα Decoy Receptor-Trojan Horse Fusion Protein
Authors: Sumbria Rachita K , Boado Ruben J , Pardridge William M
Published: June 2012

Tumor necrosis factor (TNF)- α is produced in brain in response to acute cerebral ischemia, and promotes neuronal apoptosis. Biologic TNF inhibitors (TNFIs), such as the etanercept, cannot be developed as new stroke treatments because these large molecule drugs do not cross the blood–brain barrier (BBB). A BBB-penetrating biologic TNFI was engineered by fusion of the type II human TNF receptor (TNFR) to each heavy chain of a genetically engineered chimeric monoclonal antibody (MAb) against the mouse transferrin receptor (TfR), designated as cTfRMAb-TNFR fusion protein. The cTfRMAb domain of the fusion protein acts as a molecular Trojan horse to deliver the fused TNFR across the BBB. Etanercept or the cTfRMAb-TNFR fusion protein (1 mg/kg) was administered intravenously in adult mice subjected to 1-hour reversible middle cerebral artery occlusion up to 90 minutes after the occlusion. Neuroprotection was assessed at 24 hours or 7 days after occlusion. The cTfRMAb-TNFR fusion protein treatment caused a significant 45%, 48%, 42%, and 54% reduction in hemispheric, cortical, and subcortical stroke volumes, and neural deficit, respectively. Intravenous etanercept had no therapeutic effect. Biologic TNFIs can be reengineered for BBB penetration, and the IgG-TNFR fusion protein is therapeutic after delayed intravenous administration in experimental stroke.
Refined Analysis of the Critical Age Ranges of Childhood Overweight: Implications for Primary Prevention
Authors: Hoffmann Sascha W. , Ulrich Rolf , Simon Perikles
Published: June 2012

Prevention‐interventions would certainly benefit from a precise knowledge of the age range when the most pronounced increases in prevalence of overweight and obesity occur in the general population. Data of 15,662 subjects aged 2–18 years were obtained from a national representative health survey (German Health Interview and Examination Survey for Children and Adolescents (KiGGS)) conducted in Germany. Weight, height, and BMI z‐scores were calculated relative to the UK 1990 reference, and prevalence of overweight and obesity was defined according to the IOTF (International Obesity Task Force) age‐ and sex‐specific cut‐offs. Univariate ANOVAs for overweight, obesity, weight, height, and BMI z‐scores as dependent variables were employed to assess significant differences for these measures across various age levels. Significant analysis was followed by post‐hoc comparisons using Bonferroni adjustments. The main effect of age was estimated using a multinomial logistic regression model, and by defining the first derivative of a polynomial spline function. Different eclectic slopes over the entire age range from 2 to 18 years have been observed. Prevalence of overweight substantially increases between the 5th and the 8th year (12.5–21.4% P ≤ 0.001). Maximum increase of the polynomial fit was detected at 7.2 years. Our findings suggest a relatively narrow age range at the first school year when overweight in German children especially increases. We therefore propose that psychosocial correlates may be related to the general life‐time event around the age of entering school.
Characterization of secretory sphingomyelinase activity, lipoprotein sphingolipid content and LDL aggregation in ldlr−/− mice fed on a high-fat diet
Authors: Deevska Gergana M. , Sunkara Manjula , Morris Andrew J. , Nikolova-Karakashian Mariana N.
Published: June 2012

The propensity of LDLs (low-density lipoproteins) for aggregation and/or oxidation has been linked to their sphingolipid content, specifically the levels of SM (sphingomyelin) and ceramide. To investigate this association in vivo, ldlr (LDL receptor)-null mice (ldlr−/−) were fed on a modified (atherogenic) diet containing saturated fats and cholesterol. The diet led to significantly elevated SM content in all serum lipoproteins. In contrast, ceramide increased only in the LDL particles. MS-based analyses of the lipid acyl chain composition revealed a marked elevation in C16:0 fatty acid in SM and ceramide, consistent with the prevalence of palmitic acid in the modified diet. The diet also led to increased activity of the S-SMase [secretory SMase (sphingomyelinase)], a protein that is generated by ASMase (acid SMase) and acts on serum LDL. An increased macrophage secretion seemed to be responsible for the elevated S-SMase activity. ASMase-deficient mice (asm−/−/ldlr−/−) lacked S-SMase activity and were protected from diet-induced elevation in LDL ceramide. LDL from asm−/−/ldlr−/− mice fed on the modified diet were less aggregated and oxidized than LDL from asm+/+/ldlr−/− mice. When tested in vitro, the propensity for aggregation was dependent on the SM level: only LDL from animals on modified diet that have high SM content aggregated when treated with recombinant S-SMase. In conclusion, LDL-SM content and S-SMase activity are up-regulated in mice fed on an atherogenic diet. S-SMase mediates diet-induced changes in LDL ceramide content and aggregation. S-SMase effectiveness in inducing aggregation is dependent on diet-induced enrichment of LDL with SM, possibly through increased hepatic synthesis.
Apport des reconstitutions collées dans l’esthétique du secteur
antérieur : une gestion de 7 à 77 ans
Authors: Bonnet Eric
Published: June 2012

L’esthétique n’est pas un vain mot : il est lourd de sens tant pour les patients que pour
les praticiens qui collaborent régulièrement avec cette « science ». Le niveau d’exigence
est devenu si important qu’il nécessite la participation active de plusieurs disciplines
dans le domaine de l’odontologie afin de pouvoir obtenir le résultat tant attendu par nos
patients. L’orthodontie, la stratification des composites, les techniques de collage, les
préparations minimalement invasives, les céramo-céramiques représentent autant d’éléments
qui nous permettent de répondre à la demande esthétique croissante de nos contemporains.
Si cette demande n’a intéressé que les jeunes adultes pendant de nombreuses années, avec
l’allongement de la durée de vie, les « grands » adultes ont aujourd’hui rejoint l’ensemble
de la communauté « esthétique ». Une parfaite connaissance des indications thérapeutiques,
un profond respect des différents temps de traitement ainsi que des différentes solutions
techniques permettra alors de répondre favorablement à ces sollicitations.
Traversing the ‘Particular’ through the ‘Universal’: The Politics of Negotiating Violent Masculinities in Cambodia
Authors: Lilja Mona
Published: June 2012

The article analyses programmes against gender-based violence (GBV) in Cambodia in order to understand what notions of power, agency and resistance reside within these programmes. The text relies on in-depth interviews with four different organisations in Cambodia. The interviews display a number of hands-on practices of resistance against GBV, which require a broad discussion of identity in order to be fully understood. In particular, the organisations emphasize the importance of approaching men—in men's groups, as trainers and role models—in the resistance against GBV. In their approach to Cambodian men, the trainers mixed representations of a more ‘particular’ character with representations of a more ‘universal’ appearance. Both in the establishment of new subject positions and new discourses, the Cambodian trainers leaned upon and alternated between universal and particular notions. In addition, men's ‘particular’ subject positions became the very lens through which they considered ‘universal’ notions of violent masculinities. New aspects of the resistance against GBV thus become visible as the concepts of universalism and particularism are put in use. It is in the nexus between ‘universal’ and ‘particular’ representations that a non-violent masculinity is fostered.
Trans- Bodies in/of War(s): Cisprivilege and Contemporary Security Strategy
Authors: Shepherd Laura J. , Sjoberg Laura
Published: June 2012

This article explores a gendered dimension of war and conflict analysis that has up until now received little attention at the intersection of gender studies and studies of global politics: queer bodies in, and genderqueer significations of, war and conflict. In doing so, the article introduces the concept of cisprivilege to International Relations as a discipline and security studies as a core sub-field. Cisprivilege is an important, but under-explored, element of the constitution of gender and conflict. Whether it be in controversial reactions to the suggestion of United Nations Special Rapporteur Martin Scheinin that airport screenings for terrorists not discriminate against transgendered people, or in structural violence that is ever-present in the daily lives of many individuals seeking to navigate the heterosexist and cissexist power structures of social and political life, war and conflict is embodied and reifies cissexism. This article makes two inter-related arguments: first, that both the invisibility of genderqueer bodies in historical accounts of warfare and the visibility of genderqueer bodies in contemporary security strategy are forms of discursive violence; and second, that these violences have specific performative functions that can and should be interrogated. After constructing these core arguments, the article explores some of the potential benefits of an interdisciplinary research agenda that moves towards the theorisation of cisprivilege in security theory and practice.
Gender at the Crossroad of Conflict: Tsunami and Peace in Post-2005 Aceh
Authors: Lee-Koo Katrina
Published: June 2012

After the devastating tsunami hit the northern Sumatran coastline in December 2004, the Indonesian province of Aceh found itself at a crossroad. This crossroad intersected the three-decade-long civil war, the move towards peace and the need for post-disaster recovery. This article analyses the gendered politics embedded in Aceh's navigation through this crossroad. First, it argues that both the conflict and the subsequent peace process were marginalised by the international programmes of post-tsunami recovery. Second, it demonstrates that within this marginalisation, women's investments in both war and peace were further neglected throughout the formal peace process. Third, it highlights how the peace process reflected a narrow, masculinist and public sphere agenda that silenced both women and the gendered issues affecting them. In short, this article seeks to unveil the gendered politics of war and peace in post-tsunami Aceh. It does so with the feminist ambition of demonstrating that sustainable and comprehensive peace in Aceh cannot be secured without recognising and accounting for the impact that the conflict has upon gendered identities.
‘It was Better during the War’: Narratives of Everyday Violence in a Palestinian Refugee Camp
Authors: Latif Nadia
Published: June 2012

The distinction between what is commonly regarded as the routine of impoverishment and what is acknowledged and remarked upon as violence is increasingly being questioned in scholarship and public policy circles. Interrogating the distinction between routine and remarkable not only reveals the habits and relationships constituting everyday life as the site of violence, but also foregrounds questions of gender. Given that the everyday is shaped by a given community's norms regarding the gendered division of labour that produces and reproduces the conditions of the everyday, in what ways is violence as well as its experience gendered? This article examines this question in the particular context of Palestinian camp refugees’ lived experience of forced displacement in Lebanon. It explores the ways in which the violence used against Palestinian camp refugees draws on norms regarding masculinity and femininity shared by the refugees as well as their Lebanese oppressors. It also examines the ways in which Palestinian camp refugees’ everyday experience of impoverishment as well as the acknowledged violence of forced displacement, subjection to Lebanese military intelligence control, and participation in the armed struggle for national liberation are constituted by and constitutive of unequal subject positions of gender, class and citizenship.
Active (not passive) Spatial Imagery Primes Temporal Judgements
Authors: Sullivan Jessica L. , Barth Hilary C.
Published: June 2012

Previous research has shown that primes that induce particular spatial perspectives can influence temporal judgements. However, most studies have used priming stimuli that involve both spatial and motor language and imagery. Here we ask whether the motor content of these stimuli plays an important role in their ability to serve as effective primes. A total of 198 adult participants made temporal judgements after exposure to spatial primes involving varying levels of imagined effort. Spatial primes involving imagined motor actions, but not those involving equivalent passive motions through space, successfully primed decisions about time. This suggests that motor content, rather than spatial content alone, contributes to the priming effects that arise when people make temporal judgements after exposure to particular spatial perspectives.
Contracting convex immersed closed plane curves with slow speed of curvature
Authors: Lin Yu-Chu , Poon Chi-Cheung , Tsai Dong-Ho
Published: June 2012

The authors study the contraction of a convex immersed plane curve with speed

1
α

k

α

\frac {1}{\alpha }k^{\alpha }

, where

α
∈
(
0
,
1
]

\alpha \in (0,1]

is a constant, and show that, if the blow-up rate of the curvature is of type one, it will converge to a homothetic self-similar solution. They also discuss a special symmetric case of type two blow-up and show that it converges to a translational self-similar solution. In the case of curve shortening flow (i.e., when

α
=
1

\alpha =1

), this translational self-similar solution is the familiar “Grim Reaper” (a terminology due to M. Grayson).
Intrinsic volumes of random polytopes with vertices on the boundary of a convex body
Authors: Böröczky Károly , Fodor Ferenc , Hug Daniel
Published: June 2012

Let

K
K

be a convex body in

R

d

\mathbb {R}^d

, let

j
∈
{
1
,
…
,
d
−
1
}

j\in \{1, \ldots , d-1\}

, and let

ϱ
\varrho

be a positive and continuous probability density function with respect to the

(
d
−
1
)

(d-1)

-dimensional Hausdorff measure on the boundary

∂
K

\partial K

of

K
K

. Denote by

K
n

K_n

the convex hull of

n
n

points chosen randomly and independently from

∂
K

\partial K

according to the probability distribution determined by

ϱ
\varrho

. For the case when

∂
K

\partial K

is a

C
2

C^2

submanifold of

R

d

\mathbb {R}^d

with everywhere positive Gauss curvature, M. Reitzner proved an asymptotic formula for the expectation of the difference of the

j
j

th intrinsic volumes of

K
K

and

K
n

K_n

, as

n
→
∞

n\to \infty

. In this article, we extend this result to the case when the only condition on

K
K

is that a ball rolls freely in

K
K

.
Free and Bound Phenolic Acids and Total Phenolics in Black, Blue, and Yellow Barley and Their Contribution to Free Radical Scavenging Capacity
Authors: Abdel‐Aal El‐Sayed M. , Choo Thin‐Miew , Dhillon Simarata , Rabalski Iwona
Published: June 2012

Barley is considered a healthy food because of its high content of β‐glucan and phenolic antioxidants. In the current study, 28 black, blue, and yellow barleys were investigated in terms of their composition of free and bound phenolic acids and 2,2‐diphenyl‐1‐picrylhydrazyl radical scavenging capacity. Free phenolics were based on aqueous methanol extraction, whereas bound phenolics were extracted following alkaline hydrolysis. Phenolics were then separated and quantified by liquid chromatography and the Folin–Ciocalteu method. Significant differences were observed between the three barley color groups, and within each color group a wide range of phenolics concentrations existed. Ferulic acid was the predominant phenolic acid in free and bound extracts, followed by p‐coumaric acid in all the barleys investigated. Total phenols content and individual phenolic acids strongly correlated with free radical scavenging capacity of barley. Black and blue barley were found to be related and distinct from yellow barley. The results showed significant variations in phenolics among barleys, with a potential for the development of barley grains with high content of phenolic compounds as antioxidant potential.
Necrosis-Inducing Proteins ofRhynchosporium commune, Effectors in Quantitative Disease Resistance
Authors: Kirsten S. , Navarro-Quezada A. , Penselin D. , Wenzel C. , Matern A. , Leitner A. , Baum T. , Seiffert U. , Knogge W.
Published: June 2012

The barley pathogen Rhynchosporium commune secretes necrosis-inducing proteins NIP1, NIP2, and NIP3. Expression analysis revealed that NIP1 transcripts appear to be present in fungal spores already, whereas NIP2 and NIP3 are synthesized after inoculation of host plants. To assess the contribution of the three effector proteins to disease development, deletion mutants were generated. The development of these fungal mutants on four barley cultivars was quantified in comparison with that of the parent wild-type strain and with two fungal strains failing to secrete an “active” NIP1 avirulence protein, using quantitative polymerase chain reaction as well as microscopic imaging after fungal green fluorescent protein tagging. The impact of the three deletions varied quantitatively depending on the host genotype, suggesting that the activities of the fungal effectors add up to produce stronger growth patterns and symptom development. Alternatively, recognition events of differing intensities may be converted into defense gene expression in a quantitative manner.
Transgenic Expression ofTobacco mosaic virusCapsid and Movement Proteins Modulate Plant Basal Defense and Biotic Stress Responses inNicotiana tabacum
Authors: Conti G. , Rodriguez M. C. , Manacorda C. A. , Asurmendi S.
Published: June 2012

Plant viruses cause metabolic and physiological changes associated with symptomatic disease phenotypes. Symptoms involve direct and indirect effects, which result in disruption of host physiology. We used transgenic tobacco expressing a variant of Tobacco mosaic virus (TMV) coat protein (CPT42W) or movement protein (MP), and a hybrid line (MP×CPT42W) that coexpresses both proteins, to study the plant response to individual viral proteins. Findings employing microarray analysis of MP×CPT42Wplants and silenced mp×cpT42W* controls revealed that altered transcripts were mostly downregulated, suggesting a persistent shut-off due to MP×CPT42Wexpression. Next, we showed that MP triggered reactive oxygen species (ROS) accumulation, reduction of total ascorbate, and expression of ROS scavenging genes. These effects were enhanced when both proteins were coexpressed. MP and MP×CPT42Wplants showed increased levels of salicylic acid (SA) and SA-responsive gene expression. Furthermore, these effects were partially reproduced in Nicotiana benthamiana when GMP1 transcript was silenced. CPT42Wseems to be playing a negative role in the defense response by reducing the expression of PR-1 and RDR-1. MP and MP×CPT42Wtransgenic expression promoted a recovery-like phenotype in TMV RNA infections and enhanced susceptibility to Pseudomonas syringae and Sclerotinia sclerotiorum. The individual effects of viral proteins may reflect the ability of a virus to balance its own virulence.
First Report of Didymella bryoniae Causing Gummy Stem Blight of Chayote in Taiwan
Authors: Tsai Y. C. , Chen J. F.
Published: June 2012

Chayote (Sechium edule (Jacq.) Swartz, Cucurbitaceae), originally native to Mexico, is an important vegetable known as “dragon-whisker vegetable” and is cultivated for its shoots in Ji-an, Hualien County in eastern Taiwan. In June 2010, 70 to 80% of the chayote plants grown in Ji-an developed necrotic spots on stems, leaves, and fruits. The disease was severe during the warm and rainy season from June to August. The symptoms on stems, leaves, and fruits were water-soaked lesions that eventually dried up, cracked, and produced perithecia on necrotic tissues. A single ascospore was isolated from perithecia harvested from diseased stems and cultured on potato dextrose agar (PDA) at 25°C for 1 month. Colonies of three isolates (SE5, SE6, and SE7) were white to olivaceous green bearing unicellular conidia measuring 2 to 5 × 3 to 10 μm, which is consistent with the morphological characteristics of Didymella bryoniae (Auersw.) Rehm (anamorph Phoma cucurbitacearum (Fr.:Fr.) Sacc.) (1,2,3). DNA of SE5, SE6, and SE7 isolates were obtained using microwave-based method (4). The internal transcribed spacer (ITS) rDNA (GenBank accessions AB714984, AB714985, and AB714986), PCR-amplified using primers ITS1 and ITS4, had 98 to 99% nucleotide sequence identity with D. bryoniae (GenBank Accession Nos. GU045304 and GU592001). A pathogenicity test was conducted in a greenhouse with temperature ranging from 20 to 30°C. Three-day-old mycelial plugs (5 × 5 mm) of the three isolates were placed on the needle-pricked wounds of stems and leaves of 36 4-month-old potted chayote plants wrapped in plastic bags to maintain 100% relative humidity for 2 days. Six days after inoculation, water-soaked lesions formed on the stems and leaves. Controls inoculated with sterile water had no symptoms. The fungus reisolated from the lesions of diseased stems and leaves had morphological characteristics of D. bryoniae. Based on the results of morphology, molecular data, and pathogenicity tests, we reported for the first time to our knowledge that gummy stem blight of chayote is caused by D. bryoniae in Taiwan.

References: (1) J. W. Huang and W. R. Hsieh. Plant Prot. Bull. 27:325, 1985. (2) A. P. Keinath et al. Phytopathology 85:364, 1995. (3) E. Punithalingam and P. Holliday. P. 332 in: Descriptions of Pathogenic Fungi and Bacteria. CMI, Kew, Surrey, UK, 1972. (4) S. R. Tendulkar et al. Biotechnol. Lett. 22:1941, 2003.
Evaluation of Spring Wheat Germplasm for Resistance to Bacterial Leaf Streak Caused by Xanthomonas campestris pv. translucens
Authors: Kandel Yuba R. , Glover Karl D. , Tande Connie A. , Osborne Lawrence E.
Published: June 2012

Bacterial leaf streak (caused by Xanthomonas campestris pv. translucens) has reemerged as a potential threat in spring wheat (Triticum aestivum) production areas in the northern Great Plains. As with other foliar bacterial diseases, chemical control under field situations is neither economical nor practical. Development of resistant genotypes will be needed for adequate management of the disease. There is currently limited information on sources of resistance in hard spring wheat germplasm. The main objective was to develop and apply a robust screening tool for evaluating germplasm against bacterial leaf streak, and to identify resistance sources for this disease. Inoculated field experiments were conducted in Brookings and Codington Counties, SD in 2009 and 2010 using a virulent local isolate (XctSD-017) inoculated after tillering stage. Forty-five hard red spring wheat genotypes with diverse genetic backgrounds were evaluated for disease severity, with ratings made at 7-day intervals from heading through dough stage. Results of this study showed clear differences in level of resistance among the 45 genotypes, with no immunity expressed. SD4205 was found resistant to the disease with the lowest area under the disease progress curve across location–years. Other genotypes showing moderate levels of resistance included SD4148, SD4176, ‘Alsen’, SD4023, ‘Faller’, SD4024, ‘Knudson’, and SD4199. Grain weight was negatively correlated to disease, as was days to heading. The resistance identified appeared to be quantitative in nature and was expressed through slower disease progress and reduced severity.
First Report of Daylily Leaf Streak Caused by Kabatiella microsticta in China
Authors: Bai Q. R. , Han S. , Xie Y. Y. , Dong R. , Gao J. , Li Y.
Published: June 2012

Daylily (Hemerocallis spp.) is an herbaceous, perennial plant, cultivated for its flowers. Daylily is sold in Asian markets as fresh or dried flowers (the flowers of some species, e.g., Hemerocallis citrina, are edible) or as the corm, which is used for medicinal purposes. In June 2011, daylily leaf streak was found in a nursery of Jilin Agricultural University, Jilin Province, China. Symptoms included water-soaked, irregular spots along the leaf midvein that turned orange to reddish brown and eventually enlarged to coalesce into extensive, necrotic streaks along the length of the leaf, as previously reported (2). Heavily infected leaves often withered and died. Four isolates were recovered from necrotic tissue of leaf spots and cultured on potato dextrose agar (PDA) at 25°C. All colonies were initially cream to peach colored and appeared slimy. With the maturation of the culture, the colonies became dark brown to black with sparse aerial hyphae. Blastic conidia formed simultaneously on intercalary or terminal, undifferentiated conidiogenous cells, and were scattered in dense sections on culture surface. When the conidia dropped from conidiogenous cell, an indistinct scar or a denticle remained. Conidia were hyaline, one-celled, smooth, ellipsoidal, and variable in size (2.73 to 6.01 × 8.45 to 19.36 μm), and all morphological characteristics were consistent with Kabatiella microsticta Bubak (syn. Aureobasidium microstictum; 2,4). The internal transcribed spacer (ITS) region of the nuclear rDNA was amplified using primers ITS4/ITS5 (1). ITS (534 bp) was identical among all four isolates (GenBank Accession No. HE798117) and 100% identical to that of K. microsticta CBS 114.64 (FJ150873). Pathogenicity was confirmed by spraying 20 seedlings of daylily, propagated in tissue-culture medium, with a conidial suspension (106 conidia/ml) of each isolate. A second set of 20 seedlings was sprayed with the same volume of sterile water as the noninoculated control treatment. Plants were grown in the greenhouse at 20 to 25°C and were covered with plastic bags to maintain humidity on the foliage for 72 h. After 5 days, the foliar symptoms described earlier for the field plants appeared on the leaves, whereas the control plants remained healthy. K. microsticta was reisolated from the leaf spots of all 20 inoculated plants. Leaf streak is the most destructive disease of daylily, and was previously reported in Japan and the United States (Illinois, Kentucky, Mississippi, Louisiana, Pennsylvania, Maryland, Virginia, Florida, North Carolina, and Georgia) (3). To our knowledge, this is the first report of the disease caused by K. microsticta in China.

References: (1) D. E. L. Cooke et al. Mycol. Res. 101:667, 1997. (2) E. J. Hermanides-Nijhof. Stud. Mycol. 15:153, 1977. (3) R. M. Leahy et al. Plant Pathology Circular No. 376, 1996. (4) P. Zalar et al. Stud. Mycol. 61:21, 2008.
Infectivity and Inoculum Production of Phytophthora ramorum on Roots of Eastern United States Oak Species
Authors: Widmer T. L. , Shishkoff N. , Dodge S. C.
Published: June 2012

Little is known about colonization of roots of trees by Phytophthora ramorum. We examined zoospore concentration and exposure time needed to infect six Quercus (oak) species and the inoculum produced from their roots. Sprouted acorns, exposed to zoospores (3,000/ml) for different times and transplanted to potting soil, were susceptible to infection within 1 h of exposure but root weights were not impacted after 4 weeks (P = 0.952). Roots of Quercus prinus seedlings, inoculated with sporangia, had 0.6 to 3.2% colonization of the total root mass after 5 months. Neither root lesions nor obvious root sloughing were observed. Inoculum threshold levels were tested by exposing radicles to varying zoospore concentrations for 24 h. Results showed that radicle infection occurred even at 1 zoospore/ml. To test inoculum production, roots were inoculated with sporangia and transplanted into pots. Periodically, samples of runoff were collected and plated on selective medium. Afterward, root segments were plated to calculate percent colonization. After 16 and 35 days, root colonization and inoculum production from oak was lower than that of Viburnum tinus, a positive control. This study shows that P. ramorum is able to infect sprouted oak acorns and produce secondary inoculum, which may be important epidemiologically.
Bacterial Canker on Kiwifruit in Italy: Anatomical Changes in the Wood and in the Primary Infection Sites
Authors: Renzi Marsilio , Copini Paul , Taddei Anna R. , Rossetti Antonio , Gallipoli Lorenzo , Mazzaglia Angelo , Balestra Giorgio M.
Published: June 2012

The bacterial canker of kiwifruit caused by Pseudomonas syringae pv. actinidiae is a severe threat to kiwifruit production worldwide. Many aspects of P. syringae pv. actinidiae biology and epidemiology still require in-depth investigation. The infection by and spread of P. syringae pv. actinidiae in xylem and phloem was investigated by carrying out artificial inoculation experiments with histological and dendrochronological analyses of naturally diseased plants in Italy. We found that the bacterium can infect host plants by entering natural openings and lesions. In naturally infected kiwifruit plants, P. syringae pv. actinidiae is present in the lenticels as well as in the dead phloem tissue beneath the lenticels, surrounded by a lesion in the periderm which appears to indicate the importance of lenticels to kiwifruit infection. Biofilm formation was observed outside and inside plants. In cases of advanced stages of P. syringae pv. actinidiae infection, neuroses of the phloem occur, which are followed by cambial dieback and most likely by infection of the xylem. Anatomical changes in wood such as reduced ring width, a drastic reduction in vessel size, and the presence of tyloses were observed within several infected sites. In the field, these changes occur only a year after the first leaf symptoms are observed suggesting a significant time lapse between primary and secondary symptoms. It was possible to study the temporal development of P. syringae pv. actinidiae-induced cambial dieback by applying dendrochronology methods which revealed that cambial dieback occurs only during the growing season.
Pectobacterium spp. Associated with Bacterial Stem Rot Syndrome of Potato in Canada
Authors: De Boer S. H. , Li X. , Ward L. J.
Published: June 2012

Pectobacterium atrosepticum, P. carotovorum subsp. brasiliensis, P. carotovorum subsp. carotovorum, and P. wasabiae were detected in potato stems with blackleg symptoms using species- and subspecies-specific polymerase chain reaction (PCR). The tests included a new assay for P. wasabiae based on the phytase gene sequence. Identification of isolates from diseased stems by biochemical or physiological characterization, PCR, and multi-locus sequence typing (MLST) largely confirmed the PCR detection of Pectobacterium spp. in stem samples. P. atrosepticum was most commonly present but was the sole Pectobacterium sp. detected in only 52% of the diseased stems. P. wasabiae was most frequently present in combination with P. atrosepticum and was the sole Pectobacterium sp. detected in 13% of diseased stems. Pathogenicity of P. wasabiae on potato and its capacity to cause blackleg disease were demonstrated by stem inoculation and its isolation as the sole Pectobacterium sp. from field-grown diseased plants produced from inoculated seed tubers. Incidence of P. carotovorum subsp. brasiliensis was low in diseased stems, and the ability of Canadian strains to cause blackleg in plants grown from inoculated tubers was not confirmed. Canadian isolates of P. carotovorum subsp. brasiliensis differed from Brazilian isolates in diagnostic biochemical tests but conformed to the subspecies in PCR specificity and typing by MLST.
Caught in the act: the first record of copulating fossil vertebrates
Authors: Joyce Walter G. , Micklich Norbert , Schaal Stephan F. K. , Scheyer Torsten M.
Published: June 2012

The behaviour of fossil organisms can typically be inferred only indirectly, but rare fossil finds can provide surprising insights. Here, we report from the Eocene Messel Pit Fossil Site between Darmstadt and Frankfurt, Germany numerous pairs of the fossil carettochelyid turtleAllaeochelys crassesculptathat represent for the first time among fossil vertebrates couples that perished during copulation. Females of this taxon can be distinguished from males by their relatively shorter tails and development of plastral kinesis. The preservation of mating pairs has important taphonomic implications for the Messel Pit Fossil Site, as it is unlikely that the turtles would mate in poisonous surface waters. Instead, the turtles initiated copulation in habitable surface waters, but perished when their skin absorbed poisons while sinking into toxic layers. The mating pairs from Messel are therefore more consistent with a stratified, volcanic maar lake with inhabitable surface waters and a deadly abyss.
Tracking and characterizing the head motion of unanaesthetized rats in positron emission tomography
Authors: Kyme Andre , Meikle Steven , Baldock Clive , Fulton Roger
Published: June 2012

Positron emission tomography (PET) is an important
in vivo
molecular imaging technique for translational research. Imaging unanaesthetized rats using motion-compensated PET avoids the confounding impact of anaesthetic drugs and enables animals to be imaged during normal or evoked behaviour. However, there is little published data on the nature of rat head motion to inform the design of suitable marker-based motion-tracking set-ups for brain imaging—specifically, set-ups that afford close to uninterrupted tracking. We performed a systematic study of rat head motion parameters for unanaesthetized tube-bound and freely moving rats with a view to designing suitable motion-tracking set-ups in each case. For tube-bound rats, using a single appropriately placed binocular tracker, uninterrupted tracking was possible greater than 95 per cent of the time. For freely moving rats, simulations and measurements of a live subject indicated that two opposed binocular trackers are sufficient (less than 10% interruption to tracking) for a wide variety of behaviour types. We conclude that reliable tracking of head pose can be achieved with marker-based optical-motion-tracking systems for both tube-bound and freely moving rats undergoing PET studies without sedation.
Leaks in the pipeline: separating demographic inertia from ongoing gender differences in academia
Authors: Shaw Allison K. , Stanton Daniel E.
Published: June 2012

Identification of the causes underlying the under-representation of women and minorities in academia is a source of ongoing concern and controversy. This is a critical issue in ensuring the openness and diversity of academia; yet differences in personal experiences and interpretations have mired it in controversy. We construct a simple model of the academic career that can be used to identify general trends, and separate the demographic effects of historical differences from ongoing biological or cultural gender differences. We apply the model to data on academics collected by the National Science Foundation (USA) over the past three decades, across all of science and engineering, and within six disciplines (agricultural and biological sciences, engineering, mathematics and computer sciences, physical sciences, psychology, and social sciences). We show that the hiring and retention of women in academia have been affected by both demographic inertia and gender differences, but that the relative influence of gender differences appears to be dwindling for most disciplines and career transitions. Our model enables us to identify the two key non-structural bottlenecks restricting female participation in academia: choice of undergraduate major and application to faculty positions. These transitions are those in greatest need of detailed study and policy development.
Non-additive and non-stationary properties in the spatial distribution of a large marine fish population
Authors: Ciannelli Lorenzo , Bartolino Valerio , Chan Kung-Sik
Published: June 2012

Density-independent and density-dependent variables both affect the spatial distributions of species. However, their effects are often separately addressed using different analytical techniques. We apply a spatially explicit regression framework that incorporates localized, interactive and threshold effects of both density-independent (water temperature) and density-dependent (population abundance) variables, to study the spatial distribution of a well-monitored flatfish population in the eastern Bering Sea. Results indicate that when population biomass was beyond a threshold a further increase in biomass-promoted habitat expansion in a non-additive fashion with water temperature. In contrast, during years of low population size, habitat occupancy was affected positively only by water temperature. These results reveal the spatial signature of intraspecific abundance distribution relationships as well as the non-additive and non-stationary responses of species spatial dynamics. Furthermore, these results underscore the importance of implementing analytical techniques that can simultaneously account for density-dependent and density-independent sources of variability when studying geographical distribution patterns.
A specific cognitive deficit within semantic cognition across a multi-generational family
Authors: Briscoe Josie , Chilvers Rebecca , Baldeweg Torsten , Skuse David
Published: June 2012

We report a study of eight members of a single family (aged 8–72 years), who all show a specific deficit in linking semantic knowledge to language. All affected members of the family had high levels of overall intelligence; however, they had profound difficulties in prose and sentence recall, listening comprehension and naming. The behavioural deficit was remarkably consistent across affected family members. Structural neuroimaging data revealed grey matter abnormalities in the left infero-temporal cortex and fusiform gyri: brain areas that have been associated with integrative semantics. This family demonstrates, to our knowledge, the first example of a heritable, highly specific abnormality affecting the interface between language and cognition in humans and has important implications for our understanding of the genetic basis of cognition.
Mother goats do not forget their kids’ calls
Authors: Briefer Elodie F. , Padilla de la Torre Monica , McElligott Alan G.
Published: June 2012

Parent–offspring recognition is crucial for offspring survival. At long distances, this recognition is mainly based on vocalizations. Because of maturation-related changes to the structure of vocalizations, parents have to learn successive call versions produced by their offspring throughout ontogeny in order to maintain recognition. However, because of the difficulties involved in following the same individuals over years, it is not clear how long this vocal memory persists. Here, we investigated long-term vocal recognition in goats. We tested responses of mothers to their kids’ calls 7–13 months after weaning. We then compared mothers’ responses to calls of their previous kids with their responses to the same calls at five weeks postpartum. Subjects tended to respond more to their own kids at five weeks postpartum than 11–17 months later, but displayed stronger responses to their previous kids than to familiar kids from other females. Acoustic analyses showed that it is unlikely that mothers were responding to their previous kids simply because they confounded them with the new kids they were currently nursing. Therefore, our results provide evidence for strong, long-term vocal memory capacity in goats. The persistence of offspring vocal recognition beyond weaning could have important roles in kin social relationships and inbreeding avoidance.
Gestating for 22 months: luteal development and pregnancy maintenance in elephants
Authors: Lueders Imke , Niemuller Cheryl , Rich Peter , Gray Charlie , Hermes Robert , Goeritz Frank , Hildebrandt Thomas B.
Published: June 2012

The corpus luteum, a temporally established endocrine gland, formed on the ovary from remaining cells of the ovulated follicle, plays a key role in maintaining the early mammalian pregnancy by secreting progesterone. Despite being a monovular species, 2–12 corpora lutea (CLs) were found on the elephant ovaries during their long pregnancy lasting on average 640 days. However, the function and the formation of the additional CLs and their meaning remain unexplained. Here, we show from the example of the elephant, the close relationship between the maternally determined luteal phase length, the formation of multiple luteal structures and their progestagen secretion, the timespan of early embryonic development until implantation and maternal recognition. Through three-dimensional and Colour Flow ultrasonography of the ovaries and the uterus, we conclude that pregnant elephants maintain active CL throughout gestation that appear as main source of progestagens. Two LH peaks during the follicular phase ensure the development of a set of 5.4 ± 2.7 CLs. Accessory CLs (acCLs) form prior to ovulation after the first luteinizing hormone (LH) peak, while the ovulatory CL (ovCL) forms after the second LH peak. After five to six weeks (the normal luteal phase lifespan), all existing CLs begin to regress. However, they resume growing as soon as an embryo becomes ultrasonographically apparent on day 49 ± 2. After this time, all pregnancy CLs grow significantly larger than in a non-conceptive luteal phase and are maintained until after parturition. The long luteal phase is congruent with a slow early embryonic development and luteal rescue only starts ‘last minute’, with presumed implantation of the embryo. Our findings demonstrate a highly successful reproductive solution, different from currently described mammalian models.
Trichogramma
parasitoids alter the metabolic physiology of
Manduca
eggs
Authors: Potter Kristen A. , Woods H. Arthur
Published: June 2012

Egg parasitoids face unique developmental constraints. First, they have exceptionally limited resources to support themselves and their siblings through three life stages. Second, they develop within the physiological system of another species, which they modify to their own ends. We examined how these constraints affect the metabolic physiology of egg parasitism, and whether parasitoids retool their host eggshell to account for their different metabolic demands. Higher-conductance eggshells allow more oxygen to reach the developing parasitoids, but also allow more water to leave the egg. We used
Manduca sexta
(Lepidoptera: Sphingidae) eggs and
Trichogramma
(Hymenoptera: Trichogrammatidae) parasitoids from southeastern AZ, USA. Compared with unparasitized
Manduca
eggs, eggs parasitized by
Trichogramma
had lower peak metabolic rates and approximately 50 per cent lower metabolic efficiency. However, developing
Trichogramma
were far more efficient than typical transfer efficiencies between tropic levels (approx. 10%). Even within a few hours of parasitization, eggs containing more
Trichogramma
had lower per-parasitoid metabolic rates, suggesting that parasitoid larvae have mechanisms for rapidly adjusting their metabolic rates based on number of siblings. Parasitoids also appear to control the conductance of their host eggshell: their different metabolic demands were mirrored by shifts in rates of water loss.
Bottom-up effects may not reach the top: the influence of ant–aphid interactions on the spread of soil disturbances through trophic chains
Authors: Lescano María Natalia , Farji-Brener Alejandro G. , Gianoli Ernesto , Carlo Tomás A.
Published: June 2012

Soil disturbances that increase nutrient availability may trigger bottom-up cascading effects along trophic chains. However, the strength and sign of these effects may depend on attributes of the interacting species. Here, we studied the effects of nutrient-rich refuse dumps of the leaf-cutting ant,
Acromyrmex lobicornis
, on the food chain composed of thistles, aphids, tending ants and aphid natural enemies. Using stable isotopes tracers, we show that the nitrogen accumulated in refuse dumps propagates upward through the studied food chain. Thistles growing on refuse dumps had greater biomass and higher aphid density than those growing in adjacent soil. These modifications did not affect the structure of the tending ant assemblage, but were associated with increased ant activity. In contrast to the expectations under the typical bottom-up cascade effect, the increase in aphid abundance did not positively impact on aphid natural enemies. This pattern may be explained by both an increased activity of tending ants, which defend aphids against their natural enemies, and the low capacity of aphid natural enemies to show numerical or functional responses to increased aphid density. Our results illustrate how biotic interactions and the response capacity of top predators could disrupt bottom-up cascades triggered by disturbances that increase resource availability.
Trophic specialization influences the rate of environmental niche evolution in damselfishes (Pomacentridae)
Authors: Litsios Glenn , Pellissier Loïc , Forest Félix , Lexer Christian , Pearman Peter B. , Zimmermann Niklaus E. , Salamin Nicolas
Published: June 2012

The rate of environmental niche evolution describes the capability of species to explore the available environmental space and is known to vary among species owing to lineage-specific factors. Trophic specialization is a main force driving species evolution and is responsible for classical examples of adaptive radiations in fishes. We investigate the effect of trophic specialization on the rate of environmental niche evolution in the damselfish, Pomacentridae, which is an important family of tropical reef fishes. First, phylogenetic niche conservatism is not detected in the family using a standard test of phylogenetic signal, and we demonstrate that the environmental niches of damselfishes that differ in trophic specialization are not equivalent while they still overlap at their mean values. Second, we estimate the relative rates of niche evolution on the phylogenetic tree and show the heterogeneity among rates of environmental niche evolution of the three trophic groups. We suggest that behavioural characteristics related to trophic specialization can constrain the evolution of the environmental niche and lead to conserved niches in specialist lineages. Our results show the extent of influence of several traits on the evolution of the environmental niche and shed new light on the evolution of damselfishes, which is a key lineage in current efforts to conserve biodiversity in coral reefs.
Using Only the DC Current Information to Detect Stator Turn Faults in Automotive Claw-Pole Generators
Authors: Cheng Siwei , Habetler Thomas
Published: June 2012

The stator turn-to-turn short circuit is an important type of fault in automotive claw-pole generators. In a typical vehicle electric power system, the built-in rectifier of the generator makes it difficult to access the ac current or voltage information, rendering conventional sequence-component-based fault-detection methods useless. To detect such fault using only the available sensor information, a dynamic model of the claw-pole generator with stator turn faults is derived in this paper to analyze how the fault would interact with the connected battery and the static full-bridge rectifier and how it would affect the generator's output voltage and current. It is found that, in the rectified generator output current, the harmonic at one-third of the rectifier ripple frequency is a robust signature of the stator turn fault. The performance of the stator turn-fault detector is demonstrated by extensive experimental results. Although the fault detector is originally proposed for claw-pole generators, it is also applicable to most polyphase ac generators with a dc-link rectifier.