bluuebunny/crossref_metadata_2025 huggingface dataset:
Description of Nesterenkonia aerolata sp. nov., an actinobacterium isolated from air of manufacturing shop in a pharmaceutical factory
Authors: Song Ming-Hui , Ma Wen-Long , Zhang Ning , He Wei , Wang Hong-Chuan , Fan Yi-Ling , Zhang Dao-Feng , Wang Shuang
Published: October 2023

During our studies on the microorganism diversity from air of manufacturing shop in a pharmaceutical factory in Shandong province, China, a Gram-stain-positive, aerobic, cocci-shaped bacterium, designated LY-0111T, was isolated from a settling dish. Strain LY-0111T grew at temperature of 10–42°C (optimum 35°C), pH of 5.0–10.0 (optimum pH 7.0) and NaCl concentration of 1–12% (optimum 0.5–3%, w/v). Based on the 16S rRNA gene sequence analysis, the strain shared the highest sequence similarities to Nesterenkonia halophila YIM 70179T (96.2%), and was placed within the radiation of Nesterenkonia species in the phylogenetic trees. The draft genome of the isolate was sequenced, which comprised 2,931,270 bp with G + C content of 66.47%. A supermatrix tree based on the gene set bac120 indicated that LY-0111T was close related to Nesterenkonia xinjiangensis YIM 70097T (16S rRNA gene sequence similarity 95.3%). Chemotaxonomic analysis indicated that the main respiratory quinones were MK-7, MK-8, and MK-9, the predominant cellular fatty acids were anteiso-C15:0 and iso-C15:0, and the major polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol. According to the phenotypic, chemotaxonomic and phylogenetic features, strain LY-0111T is considered to represent a novel species, for which the name Nesterenkonia aerolata sp. nov. is proposed. The type strain is LY-0111T (= JCM 36375T = GDMCC 1.3945T). In addition, Nesterenkonia jeotgali was proposed as a later synonym of Nesterenkonia sandarakina, according to the ANI (96.8%) and dDDH (72.9%) analysis between them.
Patient with myeloma-related kidney disease and positivity for anti-PLA2R: a case report
Authors: Li Xiaoli , Gao Peijuan , Zhang Jian , Huang Biao , Hao Yunfei
Published: October 2023

Background
Autoantibody against phospholipase A2 receptor (anti-PLA2R) has been knowledge as a specific biomarker of membranous nephropathy (MN)
Case presentation:
Herein, we found a patient with nephrotic syndrome and seropositive anti-PLA2R. Additionally, she was concurrent with multiple myeloma. Renal biopsy identified no MN but MM-related crystalline nephropathy of monoclonal light chain.
Conclusions
This is the first case with serological anti-PLA2R antibodies in patients with MM-related kidney lesions and no MN. It highlights that kidney biopsy may be still required in nephrotic patients with a positive anti-PLA2R antibody test.
Biodynamic response of the seated human body to a vibrating environment: Effect of seat backrest support
Authors: Ding Wei , Wang Leizhi , Ao Hongrui , Chen Zhaobo , Yan Hui
Published: October 2023

This paper introduces the feature that the backrest of an ergonomic chair can provide effective support to the head, chest and waist into the vibration environment, and conducts research on whether the backrest can provide the reduction to the vibration, and the influence of its stiffness and damping on the vibration reduction influence. Four-degree-of-freedom (4-DOF) human-chair coupling models are constructed to characterize the different contact modes between the head, chest, waist and backrest. The simulation results of the biodynamic response curves of the seat-to-head (STH) transfer ratio of the model agree with the trend of the experimental results, proving its validity. With backrest support, the peak of STH curve will be reduced (the effect of supporting the waist is not obvious) and its resonance frequency will be changed. When the upper torso is in full contact with the backrest, the peak value and resonant frequency of STH curve are positively correlated with the contact stiffness of the chair surface and negatively correlated with the contact damping. For periodic and nonperiodic excitation, selection methods of the contact stiffness and damping of the backrest are proposed to reduce the transmission rate and improve comfort.
FOXD subfamily genes serve as biomarkers and therapy targets in colorectal cancer
Authors: Chen Ying , Qiao Haiyan , Zhong Ruiqi , Sun Lei , Shang Bingbing
Published: October 2023

Background: The forkhead box (FOX) family of proteins regulates gene transcription and expression. It regulates various biological processes, such as tumorigenesis and cell proliferation. FOXD, a subfamily of FOX, is associated with poor prognosis. However, the potential clinical value of FOXD subfamily members has not yet been elucidated.
Methods: We used The Cancer Genome Atlas Project (TCGA), which was used to analyze the HTSeq-count data, clinical data, and single-nucleotide polymorphisms (SNPS). Furthermore, we used the DESEQ2 software to detect differentially expressed genes (DEGs). Batch survival analysis was performed using the survival and survminer packages in R to obtain genes with different expression levels. The intersection of the two results was used to identify the FOXD subfamily as the principal variable. Each gene was analyzed using RT-qPCR, western blotting, and immunohistochemistry. Functional enrichment analysis of FOXD subfamily-related DEGs was performed using the ClusterProfiler package. A protein network of FOXD subfamily-related DEGs was constructed using the STRING online database. We used CIBERSORT to determine the relationship between FOXD subfamily expression and immune cell infiltration. We established a survival analysis model to explore the clinical correlation between FOXD subfamily members and CRC.
Results: In contrast to the normal tissue/cell line, FOXD1, FOXD2, FOXD3, and FOXD4 expression was higher. No FOXD1 mutations were detected. Moreover, FOXD2 was detected in both COAD and READ groups. FOXD3 and FOXD4 were onlymutated in COAD. Among the FOXD subfamily members, the AUC of FOXD3 was 0.949, indicating that FOXD3 has a high overall diagnostic accuracy for CRC. The results of the GSEA showed that the genes related to the FOXD subfamily were mainly related to the KEGG pathway, such as cytokine, cytokine, and ECM receptor interactions. Kaplan-Meier curves and nomograms showed that FOXD1, FOXD3, and FOXD4 were prognostically significant.
Conclusions: We explored the correlation between the expression of the FOXD subfamily genes and the clinical and immunological characteristics of patients with CRC. The FOXD subfamily may serve as a diagnostic and prognostic biomarker for CRC and be used as an immunotherapy target in patients with CRC.
Beast Quake (Taylor’s Version): analysis of seismic signals recorded during two Taylor Swift concerts
Authors: Caplan-Auerbach Jacqueline , Marczewski Kyla , Bullock Gavin
Published: October 2023

Taylor Swift concerts at Lumen Field, Seattle in July 2023 were detected seismically by an accelerometer located proximal to the stadium. The signals were nearly identical over the two nights, consistent with a repeated set list. Ground shaking is recorded in two primary frequency bands: broadband energy between 30-80 Hz, and harmonic low frequencies between 1-8 Hz. Discrete songs are clearly visible within the data, with narrow-band low frequency signals matching the published tempo of each song. Signals recorded in association with the sound check contain little to no low-frequency energy, suggesting that low frequency energy is associated with the synchronous motion of a dancing crowd. High frequency energy is observed only during songs that include a full band as opposed to solo acoustic performances by Taylor Swift. Video data provided by citizen scientists in attendance at the concerts allow us to correlate changes in seismic amplitude with observations of crowd motion. The synchronized motion of 72,000 fans created sustained energy that exceeded the iconic “Beast Quake” in 2011.
Impact of Various High Fat Diets on Gene Expression and the Microbiome Across the Mouse Intestines
Authors: Martinez-Lomeli Jose , Deol Poonamjot , Deans Jonathan R , Jiang Tao , Ruegger Paul , Borneman James , Sladek Frances M.
Published: October 2023

High fat diets (HFDs) have been linked to several diseases including obesity, diabetes, fatty liver, inflammatory bowel disease (IBD) and colon cancer. In this study, we examined the impact on intestinal gene expression of three isocaloric HFDs that differed only in their fatty acid composition – coconut oil (saturated fats), conventional soybean oil (polyunsaturated fats) and a genetically modified soybean oil (monounsaturated fats). Four functionally distinct segments of the mouse intestinal tract were analyzed using RNA-seq – duodenum, jejunum, terminal ileum and proximal colon. We found considerable dysregulation of genes in multiple tissues with the different diets, including those encoding nuclear receptors and genes involved in xenobiotic and drug metabolism, epithelial barrier function, IBD and colon cancer as well as genes associated with the microbiome and COVID-19. Network analysis shows that genes involved in metabolism tend to be upregulated by the HFDs while genes related to the immune system are downregulated; neurotransmitter signaling was also dysregulated by the HFDs. Genomic sequencing also revealed a microbiome altered by the HFDs. This study highlights the potential impact of different HFDs on gut health with implications for the organism as a whole and will serve as a reference for gene expression along the length of the intestines.
Associations between Caffeine Intake and the Risk of Mortality in Adults with Prediabetes: Evidence from NHANES 1999-2018
Authors: Pan Yang , Wu Yuqing , Wu Shuting , Li Ruoyu , Hong Jing , Liu Wenyue , Zhu Hong
Published: October 2023

Background
Prediabetes is a pre-clinical disease state, in which individuals have a higher risk of developing diabetes, cardiovascular disease, and death compared to the general population. Recent studies have shown that consuming caffeine may decrease mortality risk in people with diabetes. However, it is not clear if the same effect exists in individuals with prediabetes.
Objectives
In this study, we investigated the potential impact of caffeine intake on the risk of all-cause mortality and cardiovascular mortality in adults with prediabetes.
Methods
we analyzed data from the National Health and Nutrition Examination Survey (NHANES) 1999–2018, and the vital status of participants was tracked until December 31, 2019 using the National Death Index (NDI). Caffeine intake was measured at baseline via 24-hour dietary recall. Multivariable Cox regression analysis and subgroup analysis were conducted to examine the independent relationships among caffeine intake and mortality.
Results
After approximately 20 years of follow-up, 11,581 adults with prediabetes were included, and there were 1,892 deaths, including 497 deaths due to cardiovascular diseases. The results revealed that patients in the highest quintile of caffeine intake had significantly lower hazard ratios (HRs) and 95% confidence intervals (CIs) compared to those in the lowest quintile. Specifically, the HR for all-cause mortality was 0.70 (with a 95% CI of 0.58–0.84), and the HR for cardiovascular mortality was 0.68 (with a 95% CI of 0.46–0.99).
Conclusions
Our findings indicate a positive effect of caffeine intake on reducing the risk of all-cause and cardiovascular mortality in adults with prediabetes.
Experimental and numerical analysis of the influence of fluid-structure interactions on the dynamic characteristics of a flexible tank
Authors: Tuong Bui Pham
Published: October 2023

This study evaluated the effect of two-way fluid–structure interactions (FSIs) on the dynamic characteristics of flexible storage liquid tanks. A hybrid approach, combining the finite volume method (FVM) and the finite element method (FEM), denoted as FVM/FEM, was used to model the response of a flexible water tank under seismic loading. The fluid domain was simulated using FVM, while the structural domain was represented using FEM. A two-dimensional interaction equation was solved at the contact surface between an elastic tank wall and the fluid by tuning the relaxation parameter and convergence conditions. The proposed FVM/FEM model provides fundamental insights for modeling interactions two-way FSI. The model enables evaluating the effect of considering two-way FSI on the dynamic characteristics of a tank flexible to a rigid tank wall. The accuracy of the coupling FVM/FEM method was confirmed through a comparative experiment with previous studies and design code, including the quantities of natural frequency, liquid sloshing, and hydrodynamic pressure. The results showed that the frequency of flexible tank walls differed from that of rigid walls, especially the hydrodynamic pressure of liquid motion acting on the tank. The peak hydrodynamic pressure of the fluid acting on thick-walled when considering FSI is 38.2 kPa, deviating only 0.2% from the ACI standards (38.12 kPa) or 5.47% with EC8 (36.11 kPa). However, the study shows that when considering the two-way interaction for thin-walled tanks, this deviation from ACI and EC8 increases significantly to 12.2% and 16.8%, respectively. From that, the numerical results show a “threshold value” is revealed to distinguish flexible or rigid tanks. If the tank stiffness exceeds this threshold, then the thicker the tank, the lower the hydrodynamic pressure. However, if the tank stiffness is lesser, vice versa. A good agreement is observed between the numerical, analytical, published, and experimental data.
Early combination with remdesivir, nirmatrelvir/ritonavir and sotrovimab for the treatment of COVID-19 in immunocompromised hosts
Authors: Gentile Ivan , Foggia Maria , Silvitelli Maria , Sardanelli Alessia , Cattaneo Letizia , Viceconte Giulio
Published: October 2023

Background:
Immunocompromised patients with COVID-19 have higher morbidity and mortality than general population. Some authors have successfully used antiviral combination, but never in the early phase of the infection.
Methods:
Retrospective cohort study to describe efficacy and safety of the combination of 2 antivirals, with or without a mAb, both in early (within 10 days from symptoms) and in later phase (after 10 days) of SARS-CoV-2 infection in immunocompromised patients admitted to our facility.
Results:
We treated 11 patients (7 in early phase and 4 in later phase of COVID-19) with 10 days of intravenous remdesivir plus 5 days of oral nirmatelvir/ritonavir, also combined with sotrovimab in 10/11 cases.
Notably, 100% of the “early” patients reached virological clearance at day 30 from the end of the therapy and were alive and well at follow-up, whereas corresponding figures in the “late” patients were 50% and 75%. Patients in late group more frequently needed oxygen supplementation (p=0.015) and steroid therapy (p=0.045) during admission and reached higher a COVID-19 severity (p=0.017).
Discussion:
The combination of antiviral and sotrovimab in early phase of COVID-19 in immunocompromised patients is well tolerated and associated with 100% of virological clearance. Patients treated later have lower response rate and higher disease severity, but a causative role of the therapy in such finding is yet to be demonstrated.
Corrosion behavior and mechanism of N80 steel caused by sulfate reducing bacteria in CO2-saturated shale gas field produced water at 60℃
Authors: Chen Wen , Liu Haifeng , Wu Gui-yang , Yan Jing
Published: October 2023

The corrosion of steel caused by sulfate reducing bacteria (SRB) in shale gas environment is one of the big factors leading to the service failure of pipeline steel. However, the related studies of SRB corrosion especially at a high temperature are still poor. This paper aims to investigate the effects of SRB on the corrosion behavior of N80 steel in CO2-saturated simulated shale gas produced water at 60°C by weight loss, 3D microscope,electrochemical impedance spectroscopy (EIS) and polarization curves and so on. Results showed that the corrosion rate of N80 steel in the presence of SRB was greater than that of the abiotic control specimen. SRB cells and the inhibitive organics could have a competitive adsorption on the steel surface. The adsorption of inhibitive organics on steel in the abiotic condition caused a slight corrosion of the control specimen. While the adsorption of SRB cells as well as the subsequent formation of biofilm have accelerated the localized corrosion of specimen. Therefore, steel corrosion is accelerated in the presence of SRB at a high temperature.
The inclusion of the epithelium in numerical models of the human cornea
Authors: Montanino Andrea , Pandolfi Anna
Published: October 2023

We present a patient-specific finite element model of the human cornea that accounts for the presence of the epithelium. The thin anterior layer that protects the cornea from the external actions has a scant relevance from the mechanical point of view and it has been neglected in most numerical models of the cornea, which assign to the entire cornea the mechanical properties of the stroma. Yet, modern corneal topographers capture the geometry of the epithelium, which can be naturally included into a patient-specific solid model of the cornea, treated as a multi-layer solid. For numerical applications, the presence of a thin layer on the anterior cornea requires a finer discretization and the definition of two constitutive models (including the corresponding properties) for stroma and epithelium. In this study we want to assess the relevance of the inclusion of the epithelium in the model of the cornea, by analyzing the effects in terms of uncertainties of the mechanical properties, stress distribution across the thickness, and numerical discretization.
Phylogeny and origin of diversification of Amblyomma (Acari: Ixodidae)
Authors: Uribe Juan E. , Kelava Samuel , Nava Santiago , Cotes-Perdomo Andrea P. , Castro Lyda R. , Rivera-Paéz Fredy A. , Perea Silvia , Mans Ben J. , Gofton Alexander , Teo Ernest J. M. , Zardoya Rafael , Barker Stephen C.
Published: October 2023

Background
Amblyomma is the second most diversified genus of Ixodidae that is distributed across the Indomalayan, Afrotropical, Australasian (IAA), Nearctic, and Neotropical biogeographic ecoregions, reaching in the Neotropic its higher diversity. There have been hints in previously published phylogenetic trees from mitochondrial (mt) genome, nuclear rRNA, from combinations of both and morphology that the Australasian Amblyomma or the Australasian Amblyomma plus the Amblyomma species from the southern cone of South America, might be the sister-group to the Amblyomma of the rest of the world. However, a stable phylogenetic framework of Amblyommafor a better understanding of the biogeographic patterns underpinning its diversification is lacking.
Methods
We used genomic techniques to sequence complete and nearly complete mt genomes –ca. 15 kbp– as well as the ribosomal operons –ca. 8 kbp– for 17 Amblyomma ticks in order to study the phylogeny and biogeographic pattern of the genus Amblyomma, with particular emphasis on the Neotropical region. The new genomic information generated here together with genomic information available of 43 ticks (22 other Amblyommaspecies and 21 other hard ticks –as outgroup–) were used to perform probabilistic methods of phylogenetic and biogeographic inferences and time-tree estimation using biogeographic dates.
Results
In the present paper, we present the strongest evidence yet that Australasian Amblyomma may indeed be the sister group to the Amblyomma of the rest of the world (species that occur mainly in the Neotropical and Afrotropical zoogeographic regions). Our results showed that all Amblyomma subgenera included, but Walkeriana and Amblyomma, are not monophyletic, as in the cases of Cernyomma, Anastosiella, Xiphiastor, Adenopleura, Aponomma, and Dermiomma. Likewise, our best biogeographic scenario supports the origin of Amblyomma and its posterior diversification in the southern hemisphere at 47.8 and 36.8 Mya, respectively. This diversification could be associated with the end of the connection of Australasia and Neotropical ecoregions by the Antarctic land bridge. Also, the biogeographic analyses let us see the colonization patterns of some neotropical Amblyomma species to the Nearctic.
Conclusions
We found strong evidence that the main theatre of diversification of Amblyomma was the southern hemisphere, potentially driven by the Antarctic Bridge's intermittent connection in the late Eocene. In addition, the subgeneric classification of Amblyomma lacks evolutionary support. Future studies using denser taxonomic sampling may take us to new findings on the phylogenetic relationships and biogeographic history of Amblyommagenus.
Priming of hippocampal microglia by IFN-γ/STAT1 pathway impairs social memory in mice
Authors: He Haili , Zhang Xiaomei , He Hui , Xiao Chenghong , Xu Gaojie , Li Liangyuan , Liu Yu-e , Yang Chengyan , Zhou Tao , You Zili , Zhang Jinqiang
Published: October 2023

Background: Social behavior is inextricably linked to the immune system. Although IFN-γ is known to be involved in social behavior, yet whether and how it encodes social memory remains unclear.
Methods: In current study, we injected with IFN-γ into the lateral ventricle of male C57BL/6J mice, and three-chamber social test was used to examine the effects of IFN-γ on their social preference and social memory. The morphology of microglia in the hippocampus, prelimbic cortex and amygdala were examined using immunohistochemistry, and the phenotype of microglia were examined using immunohistochemistry and enzyme-linked immunosorbent assays. The IFN-γ-injected mice were treated with lipopolysaccharide, and effects were evaluated on behavior and microglial responses. STAT1 pathway and microglia-neuron interactions were examined in vivo or in vitro using western blotting and immunohistochemistry. Finally, we use STAT1 inhibitor or minocycline to evaluated the role of STAT1 in mediating the microglial priming and effects of primed microglia in IFN-γ-induced social dysfunction.
Results: We demonstrated that 500 ng of IFN-γ injection results in significant decrease in social index and social novelty recognition index, and induces microglial priming in hippocampus, characterized by enlarged cell bodies, shortened branches, increased expression of CD68, CD86, CD74, CD11b, CD11c, CD47, IL-33, IL-1β, IL-6 and iNOS, and decreased expression of MCR1, Arg-1, IGF-1 and BDNF. This microglia subpopulation is more sensitive to LPS challenge, which characterized by more significant morphological changes and inflammatory responses, as well as induced increased sickness behaviors in mice. IFN-γ upregulated pSTAT1 and STAT1 and promoted the nuclear translocation of STAT1 in the hippocampal microglia and in the primary microglia. Giving minocycline or STAT1 inhibitor fludarabin blocked the priming of hippocampal microglia induced by IFN-γ, ameliorated the dysfunction in hippocampal microglia-neuron interactions and neural synaptic function, thereby improving social memory deficits in IFN-γ injected mice.
Conclusions: IFN-γ initiates STAT1 pathway to induce priming of hippocampal microglia, thereby disrupts hippocampal microglia-neuron interactions and neural circuit link to social memory. Blocking STAT1 pathway or inhibiting microglial priming may be strategies to reduce the effects of IFN-γ on social behavior.
Limited threat of Plasmodium falciparum pfhrp2 and pfhrp3 gene deletion to the utility 1 of HRP2-based malaria RDTs in Northern Uganda
Authors: Agaba Bosco B. , Smith David , Travis Jye , Pasay Cielo , Nabatanzi Monica , Arinaitwe Emmanuel , Ssewanyana Isaac , Nabadda Susan , Cunningham Jane , Kamya Moses R. , Cheng Qin
Published: October 2023

Background: Rapid diagnostic tests (RDTs) that detect Plasmodium falciparum histidine-rich protein-2 (PfHRP2) are exclusively deployed in Uganda however deletion of the pfhrp2/3 target gene threatens their usefulness as malaria diagnosis and surveillance tools.
Methods: A cross sectional survey was conducted in 40 sites across four regions of Uganda in Acholi, Lango, W. Nile and Karamoja from March 2021 to June 2023. Symptomatic malaria suspected patients were recruited and screened with both HRP2 and pan lactate dehydrogenase (pLDH) detecting RDTs. Dried blood spots (DBS) were collected from all patients and a random subset were used for genomic analysis to confirm parasite species and pfhrp2 and pfhrp3 gene status. Plasmodium species was determined using a conventional multiplex PCR while pfhrp2 and pfhrp3 gene deletions were determined using a real-time multiplex qPCR. Expression of the HRP2 protein antigen in a subset of samples was further assessed using a ELISA.
Results: Out of 2,435 symptomatic patients tested for malaria, 1505 (61.8%) were positive on pLDH RDT. Overall, qPCR confirmed single pfhrp2 gene deletion in 1 out of 416 (0.2%) randomly selected samples that were confirmed of P. falciparum mono-infections.
Conclusion: These findings show limited threat of pfhrp2/3 gene deletions in the survey areas suggesting that HRP2 RDTs are still useful diagnostic tools for surveillance and diagnosis of P. falciparum malaria infections in symptomatic patients in this setting. Periodic genomic surveillance is warranted to monitor the frequency and trend of gene deletions and its effect on RDTs.
Evaluation The Effects of Body Temperature Irrigation Fluid in Semirigid Ureteroscopy: A New Standard?
Authors: Karaca Yavuz , Sinanoglu Orhun , Ustun Fatih , Sahinler Emre Burak , Sahin Cahit , Sarica Kemal
Published: October 2023

The aim of this study was to show the advantages of body temperature irrigation fluid in ureteroscopy compared to room temperature fluid. A total of 94 patients with radio-opaque single ureteral stone whom scheduled for semirigid ureteroscopy were enrolled into this study. Patients were randomized into 2 groups: Group 1, ureteroscopy with room temperature irrigation fluid (20-22 ºC) and Group 2, ureteroscopy with body temperature (37 ºC) irrigation fluid. Patient characteristics (age, sex), stone characteristics (stone side, stone location, stone burden, Hounsfield unit), operation outcomes (operation time, ureteral JJ stenting, complications, stone free rate at 4 weeks, auxiliary intervention need, visual analogue score) were analyzed. There was no statistically significant difference between two groups regarding patient characteristics and stone characteristics. Operation time was found to be shorter in Group 2 compared to Group 1 (p: 0.02). Post-operative pain was also less common in Group 2 compared to Group 1 (p<0.001). Complication rates was 17% in Group 1 and 8% in Group 2 but no statistically significant difference was found. Body temperature irrigation fluid in ureteroscopy is beneficial because: it facilitates easier ureteral access by decreasing ureteral spasms, shortens operation times, lowers post-operative pain and lowers the complications rates. This method can be used in semirigid ureteroscopy since it is an easily applicable method with no known associated complications.
Adulteration of clove essential oil: detection using an electronic nose with polymeric gas sensors
Authors: Graboski Adriana Marcia , Feltes Giovana , Zakrzevski Claudio Augusto , Shimizu Flavio Makoto , Steffens Juliana , Paroul Natália , Steffens Clarice
Published: October 2023

Food adulteration is a global concern that affects almost all of the food industry. The market for clove essential oil (CEO) has also been affected by corrupt practices. As an alternative to monitoring the quality of this product, an electronic nose (e-nose) based on an array of polymeric nanocomposite gas sensors modified with different sensitive layers was applied to detect vaseline (petroleum jelly), the most common adulterant found in the CEO. Different proportions of vaseline (ranging from 3 to 100% (v/v)) were mixed with CEO samples, and the fingerprints of volatile compounds were obtained using the e-nose and fourier transform infrared-attenuated total reflectance (FTIR-ATR) spectroscopy. Collected data were analyzed using clustering analysis (CA), principal component analysis (PCA), linear discriminant analysis (LDA), and interactive document map (IDMAP) multivariate projection techniques. These were capable of discriminating between the different percentages of adulterants. The results of this study demonstrated that chemometric tools can be successfully used as e-nose and applied to detect mislabeling and adulteration of CEO, where PCA, LDA, and IDMAP showed accuracies of 99.85, 98.30, and 99.81%, respectively. The e-nose results were consistent with the findings from density and refractive index experiments. Thus, the results obtained demonstrate that the e-nose is a promising tool for analyzing CEO adulterations, proving its potential application in the food industry due to its rapid, economical, and high-performance tool.
Construction and validation of novel lactate metabolism molecular subtypes in hepatocellular carcinoma based on multi-omics analysis
Authors: Li Hongxu , Gao Jie , Li Minghao , Liu Long Zaoqu , Liu Lei , Hu Bowen , Wang Zhihui , Guo Wenzhi , Zhang ShuiJun
Published: October 2023

Purpose
Lactate, as a metabolic byproduct, has been found to promote carcinogenesis and induce immunosuppression in certain types of cancer. The objective of this study is to investigate the role of lactate metabolism-related regulatory genes in hepatocellular carcinoma (HCC).
Methods
In this study, we compiled transcriptomics data, genomic structural variation data (SNV, CNV) from The Cancer Genome Atlas (TCGA), as well as our proteomics data. We constructed the lactate metabolism score (LMScore) by integrating consensus clustering, differential analysis, and principal component analysis based on TCGA transcriptome data. Subsequently, we decoded the differences in clinical outcomes, mutation landscape, biological behaviors, immune characteristics and potential therapeutic drugs between distinct subtypes. Western blot (WB) was used to evaluate the protein levels of different tissues.
Results
The findings demonstrated a significant negative correlation between LMScore and the progression of HCC. Patients in distinct subtypes showed significant differences at multiple omics levels, such as transcriptome profiling, mutational landscape, and protein levels. Specifically, Patients in high LMScore subtype had a better prognosis and were more likely to benefit from immune therapy and clinical first- and second-line therapy including cisplatin and lenvatinib. Moreover, Patients in the low-score subtype exhibited high levels of immune cell infiltration, upregulation of immune checkpoints, and poor response of immunotherapy, suggesting an immune-suppressive microenvironment. Drug sensitivity analysis demonstrated these patients, who were not suitable for immunotherapy, could benefit more from drugs like paclitaxel and sorafenib. In addition, we found that TTC36 is a prognostic gene that not only affects the progression of HCC but also alters lactate metabolism levels. Upregulation of TTC36 can inhibit proliferation, invasion, migration, and lactate metabolism of HCC cell.
Conclusion
According to the lactate metabolism regulated genes, we constructed the LMScore, which can serve as powerful tool in stratifying HCC patients and decoding HCC heterogeneity. Moreover, this model can guide the treatment of immune therapy and clinical first- and second-line drugs, offering an optimized decision-making platform for HCC patients.
RETRACTED: Detection of invA Gene by PCR and Identification with Susceptibility Pattern Evaluation by VITEK 2 of Salmonella Isolates
Authors: Akter Fahima , Shamimuzzaman Md.
Published: October 2023

The authors have requested that this preprint be removed from Research Square.
Exploring the utility of artificial intelligence of intrapartum cardiotocography: a systematic review
Authors: Salih Mohamed , Warty Ritesh Rikain , Murday Hamsaveni Kalina , Kaushik Arjun , Zhang Yuxin , Horta Fabrizzio , Reddy Sandeep , Vollenhoven Beverley , Rezatofighi Hamid , Cheng Wenlong , Smith Vinayak
Published: October 2023

Background:Cardiotocography (CTG) interpretation is complex and highly subjective. Misinterpretation can result unnecessary, late, or inadequate intervention; potentially harming the mother or fetus. Artificial intelligence (AI) could play a role in reducing the likelihood of these incidents.Purpose:To identify the current state-of-the-art in AI models for CTG interpretation and provide clinicians and AI developers alike with an overview of this landscape and guide the development of future models.Methods:We searched PubMed, EMBASE, Ovid Medline, and IEEE Xplore for studies published from 01/06/2005 to 07/06/2020. Studies focused on AI applications to CTG were included, with the performance metrics (accuracy, sensitivity, and specificity) being extracted for analysis. PROSPERO registration: CRD42021250394.Results:38 articles were eligible for inclusion, though all studies were focused on pre-clinical performance evaluation. The types of AI techniques employed included support vector machines (11/38), decision trees (10/38), random forests (8/38), neural networks (23/38), and novel custom algorithms (11/38). Each model demonstrated promise in a pre-clinical setting although true clinical value is presently uncertain. Other issues included the classification systems used by AI developers, as well as the limited scope of these classification systems when compared to the more comprehensive clinical AI systems already implemented clinically in similar medical applications.Conclusion:AI shows promise as an adjunct surveillance tool in CTG interpretation. However, currently, it is too early to conclusively determine its implementation value in a clinical setting. To do so, these AIs need to be developed for and validated in high quality prospective clinical evaluations.
Impact of the Conjoined Tendon on Preventing Dislocation Following Direct Anterior Total Hip Arthroplasty: A Cadaver Study
Authors: zhao gongyin , ZHAO Chenyu , Bao Hongwei , Liu Junting , Zhou Baojun , Wang Yuji
Published: October 2023

Background:
Investigating the stabilizing impact of the piriformis and conjoined tendons post-direct anterior total hip arthroplasty (DATHA) forms the crux of this study, given the pertinence of hip stability in postoperative outcomes and long-term patient mobility.
Methods:
Utilizing 16 hips from 8 fresh frozen cadavers, the study establishes two cohorts: the piriformis tendon group and the conjoined tendon group. A rigorous assessment involving simulated anterior and posterior dislocations post-DATHA was implemented, quantifying the torque requisite for dislocation under three scenarios: pre-severance, post-severance, and post-reconstruction.
Results:
Piriformis tendon group analysis demonstrated inconsequential variations in torque for anterior and posterior dislocations post-tendon severance (p = 0.9801, p = 0.9854) and post-reconstruction (p = 0.2952, p = 0.0801) relative to the intact state. Conversely, the conjoined tendon group witnessed a significant diminution in dislocation torque subsequent to tendon severance (p < 0.0001), with a notable escalation post-reconstruction (p < 0.0001).
Conclusion:
This study underscores the preeminent role of the conjoined tendon in ensuring hip stability following DATHA, highlighting the cruciality of its preservation and reconstruction during surgical interventions. Such insights bear significant implications for surgical methodologies in orthopedic practice, particularly concerning minimizing postoperative dislocation risks in DATHA.
Clinical investigation on the efficacy of modified Mason-Allen in healing delaminated rotator cuff tears
Authors: Wu Runqing , Hu Gangfeng , Guan Hong , Xu Fangqi , Xu Shibing , Yu Pengzheng , Wang Longfeng , Ye Jiakuang
Published: October 2023

PurposeTo evaluate the arthroscopic modified Mason-Allen (mMA) suture technique's clinical effectiveness and safety in treating delaminated rotator cuff injuries.MethodsCollected 62 patients with delaminated rotator cuff tears repaired using arthroscopic modified Mason-Allen suture technique from May 2022 to June 2023, including 21 males and 41 females; age ranged from 50 to 81 years old, with a median age of 65 years old; 34 cases in the left shoulder and 28 cases in the right shoulder; 30 cases with a history of trauma and 32 cases with no obvious history of trauma; According to the Cofield classification of rotator cuff tears, all were delaminated tears of the rotator cuff (1 to 3 cm). We recorded changes in shoulder joint range of motion (ROM) and functional ratings from 3 to 6 months following surgery, with a median of 6 months. Follow-up visits were undertaken to assess tendon-bone healing, improvement of shoulder discomfort, recovery of shoulder joint function, and incidence of problems.ResultsAll patients were followed up for 3 to 6 months, with a median of 6 months. The MRI demonstrated excellent tendon-bone healing in all patients at the last follow-up. Shoulder anterior flexion supination improved from 98.06°±11.28°to 169.05° ± 9.05°, abduction supination improved from 69.68°±18.55°to 147.86°± 11.16°, internal rotation improved from 14.52°±10.35°to 47.62°± 6.91°, and external rotation improved from 22.42°±8.62°to 49.76°±6.04°; Constant-Murely score, preoperative (49.08 ± 4.91) and at the last follow-up (93.33 ± 4.95); and UCLA shoulder score, preoperative (15.73 ± 2.60) and at the last follow-up (33.55 ± 1.53). The change was statistically significant when comparing the data before and after surgery (P < 0.001). Eight patients acquired frozen shoulder symptoms after surgery owing to inconsistent rehabilitation activities, and the symptoms resolved after standardized rehabilitation exercises. All patients did not experience issues such as rotator cuff re-tear and anchor nail removal.ConclusionRepair with arthroscopic modified Mason-Allen suture technique can restore the wholeness and integrity of the delaminated rotator cuff, which can effectively alleviate shoulder joint pain, help early exercise, prevent and control further rotator cuff tear, enhance the initial strength of the rotator cuff as a whole, and improve the joint function of patients. Level of evidence III.
The prognosis of ablative treatments and liver resection for early-stage hepatocellular carcinoma：a systematic review and Bayesian network meta
Authors: Min Yiyang , Tong Kuinan , Lin Huajun , Wang Dong , Guo Wei , Li Shun , Zhang Zhongtao
Published: October 2023

Objective
To compare overall survival (OS) rates and disease-free survival (DFS) rates following radiofrequency ablation (RFA), microwave ablation (MWA), cryoablation (CA) and Liver resection (LR) for hepatocellular carcinoma (HCC).
Method
PubMed, SCI, and Cochrane databases were searched for literature until March 1st, 2023. The data obtained from the literature were netted using Stata 15.0 and R 4.2.3. The primary assessments of content were overall survival (OS) rates and disease-free survival (DFS) rates at 1 and 3 years. OS rates and DFS rates were reported as the hazard ratio (HR) with 95% confidence interval (95% CrI) to summarize the effect of each comparison.
Result
A total of 25 publications with 5247 patients were included, including 12 studies in mainland China and 13 in other regions. Overall outcomes: for one-year and three-year overall survival rates, there was no statistical difference between the groups compared; for one-year disease-free survival rates, the HR was 1.85 (95% CrI: 1.31–2.61) for LR compared with RFA; 1.76 (95% CrI: 1.22–2.54) for LR compared with MWA, with no statistical difference in the rest of the groups compared; for three-year disease-free survival rates, the HR was 1.92 (95% CrI: 1.38–2.66) for LR compared with RFA; 1.88 (95% CrI: 1.31–2.68) for LR compared with MWA, and the rest were not statistically significant.
Conclusion
For particularly early-stage hepatocellular carcinoma, ablative procedures have comparable therapeutic efficacy; LR is more effective in reducing tumor recurrence than interventional ablation.
Optimal Local Planner Selection for Autonomous Robots Using Machine Learning
Authors: Oner Hatice Kübrä , Sezer Volkan
Published: October 2023

Autonomous robots have been actively involved in visible and invisible aspects of our daily lives for several years. These robots can perform various tasks and roles in different locations, such as functioning as waiters in restaurants, serving as housekeepers in hotels, or transporting goods as attendants in warehouses. Autonomous robots rely on local planners to navigate in their environment. However , selecting the most suitable local planner for a given task can be a challenge. Despite extensive research in areas such as autonomous navigation, human-robot interaction, and robot localization, there has been no study to determine the optimal local planner for the given environments. In this study, we propose a machine learning solution that, for the first time in the literature, selects the optimal local planner before initiating navigation. Our approach involves using environmental metrics as inputs for a machine learning model, with the output being the name of the local planner. To create a dataset, 1280 tests were conducted within a simulation environment. The results demonstrate that the proposed solution achieves a success rate of up to 91% has been achieved in recommending the appropriate local planners based on the desired criteria.
A New Prognostic Model of Oxidative-Stress-Related lncRNAs in Hepatocellular Carcinoma & Its Validation In Vitro
Authors: Zhao Weijian , Li Yaqi , Xu Hongting , Zhang Xiaofei , He Xuesong , Xiao Xiangqian , Sheng Wang
Published: October 2023

Hepatocellular carcinoma (HCC)is one of the most common cancers and the most common primary liver cancer worldwide. Oxidative stress is believed to be closely related to the development of cancer. Long non-coding RNAs (lncRNAs) are also reported to play an important regulatory effect in carcinogenesis and malignancy. Therefore, we constructed a prognostic model for oxidative-stress-related lncRNAs to evaluate the prognosis of HCC patients and demonstrated that AC009005.1 is a carcinogenic factor which can promote the development of HCC in vitro experiments.
Neuroprotective upshot of isorhoifolin flavonoid of Gmelina asiatica for multitargeted approach in Alzheimer's disease: Exploration through in-silico studies
Authors: Andarghiske Komal Rajesh , Kancharla Bhanukiran , Siva Hemalatha
Published: October 2023

Alzheimer's is a progressive neurodegenerative disease with amyloid-beta peptide deposition that impairs memory and cognitive decline. In previous reports, the plant Gmelina asiatica was reported to have anti-Alzheimer’s disease (AD) activity. However, the responsible chemical constituents of G. asiatica for anti-Alzheimer’s activity have not been explored. Therefore, the present study aimed to investigate hit compounds through an in silico approach. The reported phytoconstituents were initially screened through auto dock software. Then, the lead compound was further evaluated through molecular dynamic studies. Further hit compound isorhoifolin physicochemical and ADMET properties were analyzed. In docking studies, isorhoifolin showed the least binding energy and well resided at the active sites of AchE (-10.9 kcal/mol), BACE1 (-10.2 kcal/mol), GSK-3 (-9.7 kcal/mol), TACE1 (-9 kcal/mol). Further molecular dynamic simulation revealed that isorhoifolin is stable up to 200 ns. Furthermore, density functional theory (DFT) was analyzed using Gaussian software, where the quantum mechanics objective was focused. Besides, the physicochemical properties of isorhoifolin were studied, such as absorption, distribution, metabolism, excretion, and toxicity, through the pkcsm software. The study suggests isorhoifolin from the Gmelina asiatica plant may be responsible for anti-Alzheimer's activity, which may be considered for further investigation.
Cash Transfers and After-School Programs: A Randomized Controlled Trial for Young Men at Risk of Violence Exposure in Wilmington, Delaware
Authors: Stacy Christina , Teles Daniel , Gonzelez Jorge , Walker Fay , Morgan Anna , Huettner Steven , Matson Pamela A.
Published: October 2023

We conducted a randomized controlled trial to determine whether an after-school program paired with a cash transfer (a conditional cash transfer) or a cash transfer alone (an unconditional cash transfer) can help improve health and economic outcomes for young men between the ages of 14 and 17 whose parents have low-incomes and who live in neighborhoods with high crime rates. We find that receiving the cash transfer alone led to an increase in healthy behaviors (one of our primary outcome composite measures) and that the cash transfer paired with after school programming improved the financial health of participants (one of our secondary outcome composite measures). We find no differences in spending on alcohol, marijuana, cigarettes, or other drugs between either treatment group and the control group. Neither the cash transfer alone nor the programming plus cash transfer had statistically significant effects on our other primary composite measures (physical and mental health or school attendance and disciplinary actions), or our other secondary composite measures (criminal justice engagement or social supports) but in most cases, confidence intervals were too large to rule out meaningful effects. Results suggest that cash transfers hold promise to improve the health of youth without any indication of any adverse effects.
The vertical mobility of the first tarsometatarsal joint during demi-plié with forced turnout in ballet dancers
Authors: Ishihara Honoka , Maeda Noriaki , Komiya Makoto , Tashiro Tsubasa , Tsutsumi Shogo , Arima Satoshi , Kawai Miki , Tamura Yuki , Ikuta Yasunari , Urabe Yukio
Published: October 2023

The forced turnout has a perceived risk of the development of hallux valgus (HV) in ballet dancers. We determined how the forced turnout affects the sagittal mobility of the first tarsometatarsal (TMT) joint, which is one of the pathogenic factors of HV development. Seventeen female ballet dancers were included and performed demi-plié in three conditions; control, functional turnout, and forced turnout. Ultrasound imaging synchronized with a three-dimensional motion analysis system was used for measuring the vertical locations of the first metatarsal and medial cuneiform (MC) to evaluate the first TMT joint mobility. Plantar displacement of MC and the first TMT joint mobility in the forced turnout was the greatest among the 3 conditions. Multiple regression analysis showed that the extent of the forcing angle was associated with the displacement of MC and the first TMT joint mobility. Evaluating the sagittal mobility of the first TMT joint in the forced turnout can assist in understanding the association between inappropriate techniques including the forced turnout and HV development in ballet dancers. Since the excessive mobility of the first TMT joint is one of the factors of HV development, the acquirement of adequate active turnout may have the potential to prevent HV development in ballet dancers.