bluuebunny/crossref_metadata_2025 huggingface dataset:
Bleeding and Thrombosis: Insights into Pathophysiology of Bothrops Venom-Related Hemostasis Disorders
Authors: Larréché Sébastien , Chippaux Jean-Philippe , Chevillard Lucie , Mathé Simon , Résière Dabor , Siguret Virginie , Mégarbane Bruno
Published: September 2021

Toxins from Bothrops venoms targeting hemostasis are responsible for a broad range of clinical and biological syndromes including local and systemic bleeding, incoagulability, thrombotic microangiopathy and macrothrombosis. Beyond hemostais disorders, toxins are also involved in the pathogenesis of edema and in most complications such as hypovolemia, cardiovascular collapse, acute kidney injury, myonecrosis, compartmental syndrome and superinfection. These toxins can be classified as enzymatic proteins (snake venom metalloproteinases, snake venom serine proteases, phospholipases A2 and L-amino acid oxidases) and non-enzymatic proteins (desintegrins and C-type lectin proteins). Bleeding is due to a multifocal toxicity targeting vessels, platelets and coagulation factors. Vessel damage due to the degradation of basement membrane and the subsequent disruption of endothelial cell integrity under hydrostatic pressure and tangential shear stress is primarily responsible for bleeding. Hemorrhage is promoted by thrombocytopenia, platelet hypoaggregation, consumption coagulopathy and fibrin(ogen)olysis. Onset of thrombotic microangiopathy is probably due to the switch of endothelium to a prothrombotic phenotype with overexpression of tissue factor and other pro-aggregating biomarkers in association with activation of platelets and coagulation. Thrombosis involving large-caliber vessels in B. lanceolatus envenomation remains a unique entity, which exact pathophysiology remains poorly understood.
New Advances in the Research of Resistance to Neoadjuvant Chemotherapy in Breast Cancer
Authors: An Junsha , Peng Cheng , Tang Hailin , Liu Xiuxiu , Peng Fu
Published: September 2021

Breast cancer has an extremely high incidence in women, and its morbidity and mortality rank first among female tumors. With the increasing development of medicine today, the clinical application of neoadjuvant chemotherapy has brought new hope to the treatment of breast cancer. Although the efficacy of neoadjuvant chemotherapy has been confirmed, drug resistance is one of the main reasons for its treatment failure, contributing to the difficulty in the treatment of breast cancer. This article focuses on multiple mechanisms of action and expounds a series of recent research advances that mediate drug resistance in breast cancer cells. Drug metabolizing enzymes can mediate a catalytic reaction to inactivate chemotherapeutic drugs and develop drug resistance. The drug efflux system can reduce the drug concentration in breast cancer cells. The combination of glutathione detoxification system and platinum drugs can cause breast cancer cells to be insensitive to drugs. Changes in drug targets have led to poorer efficacy of HER2 receptor inhibitors. Moreover, autophagy, epithelial–mesenchymal transition, and tumor microenvironment can all contribute to the development of resistance in breast cancer cells. Based on the relevant research on the existing drug resistance mechanism, the current treatment plan for reversing the resistance of breast cancer to neoadjuvant chemotherapy is explored, and the potential drug targets are analyzed, aiming to provide a new idea and strategy to reverse the resistance of neoadjuvant chemotherapy drugs in breast cancer.
Deletion of Cdk5 in Macrophages Ameliorates Anti-Inflammatory Response during Endotoxemia through Induction of C-Maf and Il-10
Authors: Pfänder Pauline , Eiers Ann-Kathrin , Burret Ute , Vettorazzi Sabine
Published: September 2021

Immune response control is critical as excessive cytokine production can be detrimental and damage the host. Interleukin-10 (Il-10), an anti-inflammatory cytokine produced primarily by macrophages, is a key regulator that counteracts and controls excessive inflammatory response. Il-10 expression is regulated through the transcription factor c-Maf. Another regulator of Il-10 production is p35, an activator of the cyclin-dependent kinase 5 (Cdk5), which decreases Il-10 production in macrophages, thus increasing inflammation. However, Cdk5 regulation of c-Maf and the involvement of Il-10 production in macrophages has not yet been investigated. We used in vitro primary bone marrow-derived macrophages (BMDMs) lacking Cdk5, stimulated them with lipopolysaccharid (LPS) and observed increased levels of c-Maf and Il-10. In an in vivo mouse model of LPS-induced endotoxemia, mice lacking Cdk5 in macrophages showed increased levels of c-Maf and elevated levels of Il-10 in lungs as well as in plasma, resulting in ameliorated survival. Taken together, we identified Cdk5 as a potential novel regulator of Il-10 production through c-Maf in macrophages under inflammatory conditions. Our results suggest that inhibition of Cdk5 enhances the c-Maf-Il-10 axis and thus potentiates improvement of anti-inflammatory therapy.
JNK Signaling in Drosophila Aging and Longevity
Authors: Gan Tian , Fan Lixia , Zhao Long , Misra Mala , Liu Min , Zhang Min , Su Ying
Published: September 2021

The evolutionarily conserved c-Jun N-terminal kinase (JNK) signaling pathway is a critical genetic determinant in the control of longevity. In response to extrinsic and intrinsic stresses, JNK signaling is activated to protect cells from stress damage and promote survival. In Drosophila, global JNK upregulation can delay aging and extend lifespan, whereas tissue/organ-specific manipulation of JNK signaling impacts lifespan in a context-dependent manner. In this review, focusing on several tissues/organs that are highly associated with age-related diseases—including metabolic organs (intestine and fat body), neurons, and muscles—we summarize the distinct effects of tissue/organ-specific JNK signaling on aging and lifespan. We also highlight recent progress in elucidating the molecular mechanisms underlying the tissue-specific effects of JNK activity. Together, these studies highlight an important and comprehensive role for JNK signaling in the regulation of longevity in Drosophila.
Multiple Imputation Approaches Applied to the Missing Value Problem in Bottom-Up Proteomics
Authors: Gardner Miranda L. , Freitas Michael A.
Published: September 2021

Analysis of differential abundance in proteomics data sets requires careful application of missing value imputation. Missing abundance values widely vary when performing comparisons across different sample treatments. For example, one would expect a consistent rate of “missing at random” (MAR) across batches of samples and varying rates of “missing not at random” (MNAR) depending on the inherent difference in sample treatments within the study. The missing value imputation strategy must thus be selected that best accounts for both MAR and MNAR simultaneously. Several important issues must be considered when deciding the appropriate missing value imputation strategy: (1) when it is appropriate to impute data; (2) how to choose a method that reflects the combinatorial manner of MAR and MNAR that occurs in an experiment. This paper provides an evaluation of missing value imputation strategies used in proteomics and presents a case for the use of hybrid left-censored missing value imputation approaches that can handle the MNAR problem common to proteomics data.
Identification and Engineering of Aptamers for Theranostic Application in Human Health and Disorders
Authors: Basu Debleena , Chakraborty Sourabrata , Pal Riddhi , Sharma Tarun Kumar , Sarkar Siddik
Published: September 2021

An aptamer is a short sequence of synthetic oligonucleotides which bind to their cognate target, specifically while maintaining similar or higher sensitivity compared to an antibody. The in-vitro selection of an aptamer, applying a conjoining approach of chemistry and molecular biology, is referred as Systematic Evolution of Ligands by Exponential enrichment (SELEX). These initial products of SELEX are further modified chemically in an attempt to make them stable in biofluid, avoiding nuclease digestion and renal clearance. While the modification is incorporated, enough care should be taken to maintain its sensitivity and specificity. These modifications and several improvisations have widened the window frame of aptamer applications that are currently not only restricted to in-vitro systems, but have also been used in molecular imaging for disease pathology and treatment. In the food industry, it has been used as sensor for detection of different diseases and fungal infections. In this review, we have discussed a brief history of its journey, along with applications where its role as a therapeutic plus diagnostic (theranostic) tool has been demonstrated. We have also highlighted the potential aptamer-mediated strategies for molecular targeting of COVID-19. Finally, the review focused on its future prospective in immunotherapy, as well as in identification of novel biomarkers in stem cells and also in single cell proteomics (scProteomics) to study intra or inter-tumor heterogeneity at the protein level. Small size, chemical synthesis, low batch variation, cost effectiveness, long shelf life and low immunogenicity provide advantages to the aptamer over the antibody. These physical and chemical properties of aptamers render them as a strong biomedical tool for theranostic purposes over the existing ones. The significance of aptamers in human health was the key finding of this review.
Alteration of the Early Development Environment by Maternal Diet and the Occurrence of Autistic-like Phenotypes in Rat Offspring
Authors: Gawlińska Kinga , Gawliński Dawid , Kowal-Wiśniewska Ewelina , Jarmuż-Szymczak Małgorzata , Filip Małgorzata
Published: September 2021

Epidemiological and preclinical studies suggest that maternal obesity increases the risk of autism spectrum disorder (ASD) in offspring. Here, we assessed the effects of exposure to modified maternal diets limited to pregnancy and lactation on brain development and behavior in rat offspring of both sexes. Among the studied diets, a maternal high-fat diet (HFD) disturbed the expression of ASD-related genes (Cacna1d, Nlgn3, and Shank1) and proteins (SHANK1 and TAOK2) in the prefrontal cortex of male offspring during adolescence. In addition, a maternal high-fat diet induced epigenetic changes by increasing cortical global DNA methylation and the expression of miR-423 and miR-494. As well as the molecular changes, behavioral studies have shown male-specific disturbances in social interaction and an increase in repetitive behavior during adolescence. Most of the observed changes disappeared in adulthood. In conclusion, we demonstrated the contribution of a maternal HFD to the predisposition to an ASD-like phenotype in male adolescent offspring, while a protective effect occurred in females.
Oncogenes, Proto-Oncogenes, and Lineage Restriction of Cancer Stem Cells
Authors: Brown Geoffrey
Published: September 2021

In principle, an oncogene is a cellular gene (proto-oncogene) that is dysfunctional, due to mutation and fusion with another gene or overexpression. Generally, oncogenes are viewed as deregulating cell proliferation or suppressing apoptosis in driving cancer. The cancer stem cell theory states that most, if not all, cancers are a hierarchy of cells that arises from a transformed tissue-specific stem cell. These normal counterparts generate various cell types of a tissue, which adds a new dimension to how oncogenes might lead to the anarchic behavior of cancer cells. It is that stem cells, such as hematopoietic stem cells, replenish mature cell types to meet the demands of an organism. Some oncogenes appear to deregulate this homeostatic process by restricting leukemia stem cells to a single cell lineage. This review examines whether cancer is a legacy of stem cells that lose their inherent versatility, the extent that proto-oncogenes play a role in cell lineage determination, and the role that epigenetic events play in regulating cell fate and tumorigenesis.
Ginsenoside Prolongs the Lifespan of C. elegans via Lipid Metabolism and Activating the Stress Response Signaling Pathway
Authors: Yu Xiaoxuan , Li Hui , Lin Dongfa , Guo Weizhuo , Xu Zhihao , Wang Liping , Guan Shuwen
Published: September 2021

Panax ginseng is a valuable traditional Chinese medicine in Northeast China. Ginsenoside, the active component of ginseng, has not been investigated much for its effects on aging and its underlying mechanism(s) of action. Here, we investigated the effects of total ginsenoside (TG), a mixture of the primary active ginsenosides from Panax ginseng, on the lifespan of Caenorhabditis elegans (C. elegans). We found that TG extended the lifespan of C. elegans and reduced lipofuscin accumulation. Moreover, TG increased the survival of C. elegans in response to heat and oxidative stress via the reduction of ROS. Next, we used RNA-seq to fully define the antiaging mechanism(s) of TG. The KEGG pathway analysis showed that TG can prolong the lifespan and is involved in the longevity regulating pathway. qPCR showed that TG upregulated the expression of nrh-80, daf-12, daf-16, hsf-1 and their downstream genes. TG also reduced the fat accumulation and promoted lipid metabolism. Moreover, TG failed to extend the lifespan of daf-16 and hsf-1 mutants, highlighting their role in the antiaging effects of TG in C. elegans. The four main constitution of TG were then confirmed by HPLC and included ginsenoside Re, Rg1, Rg2 and Rd. Of the ginsenosides, only ginsenoside Rd prolonged the lifespan of C. elegans to levels comparable to TG. These findings provided mechanistic insight into the antiaging effects of ginsenoside in C. elegans.
Pharmaceuticals Removal by Adsorption with Montmorillonite Nanoclay
Authors: Kryuchkova Marina , Batasheva Svetlana , Akhatova Farida , Babaev Vasily , Buzyurova Daina , Vikulina Anna , Volodkin Dmitry , Fakhrullin Rawil , Rozhina Elvira
Published: September 2021

The problem of purifying domestic and hospital wastewater from pharmaceutical compounds is becoming more and more urgent every year, because of the continuous accumulation of chemical pollutants in the environment and the limited availability of freshwater resources. Clay adsorbents have been repeatedly proposed as adsorbents for treatment purposes, but natural clays are hydrophilic and can be inefficient for catching hydrophobic pharmaceuticals. In this paper, a comparison of adsorption properties of pristine montmorillonite (MMT) and montmorillonite modified with stearyl trimethyl ammonium (hydrophobic MMT-STA) towards carbamazepine, ibuprofen, and paracetamol pharmaceuticals was performed. The efficiency of adsorption was investigated under varying solution pH, temperature, contact time, initial concentration of pharmaceuticals, and adsorbate/adsorbent mass ratio. MMT-STA was better than pristine MMT at removing all the pharmaceuticals studied. The adsorption capacity of hydrophobic montmorillonite to pharmaceuticals decreased in the following order: carbamazepine (97%) > ibuprofen (95%) > paracetamol (63–67%). Adsorption isotherms were best described by Freundlich model. Within the pharmaceutical concentration range of 10–50 µg/mL, the most optimal mass ratio of adsorbates to adsorbents was 1:300, pH 6, and a temperature of 25 °C. Thus, MMT-STA could be used as an efficient adsorbent for deconta×ating water of carbamazepine, ibuprofen, and paracetamol.
Water Dynamics in Whey-Protein-Based Composite Hydrogels by Means of NMR Relaxometry
Authors: Ozel Baris , Kruk Danuta , Wojciechowski Milosz , Osuch Maciej , Oztop Mecit Halil
Published: September 2021

Whey-protein-isolate-based composite hydrogels with encapsulated black carrot (Daucus carota) extract were prepared by heat-induced gelation. The hydrogels were blended with gum tragacanth, pectin and xanthan gum polysaccharides for modulating their properties. 1H spin-lattice relaxation experiments were performed in a broad frequency range, from 4 kHz to 30 MHz, to obtain insight into the influence of the different polysaccharides and of the presence of black carrot on dynamical properties of water molecules in the hydrogel network. The 1H spin-lattice relaxation data were decomposed into relaxation contributions associated with confined and free water fractions. The population of the confined water fraction and the value of the translation diffusion coefficient of water molecules in the vicinity of the macromolecular network were quantitatively determined on the basis of the relaxation data. Moreover, it was demonstrated that the translation diffusion is highly anisotropic (two-dimensional, 2D).
Anti-Inflammatory Effects of Metabolites from Antarctic Fungal Strain Pleosporales sp. SF-7343 in HaCaT Human Keratinocytes
Authors: Dong Linsha , Kim Hye Jin , Cao Thao Quyen , Liu Zhiming , Lee Hwan , Ko Wonmin , Kim Youn-Chul , Sohn Jae Hak , Kim Tai Kyoung , Yim Joung Han , Lee Dong-Sung , Oh Hyuncheol
Published: September 2021

Chemical investigation of the Antarctic fungi Pleosporales sp. SF-7343 revealed four known secondary fungal metabolites: alternate C (1), altenusin (2), alternariol (3), and altenuene (4). The compound structures were identified primarily by NMR and MS analyses. Atopic dermatitis, an inflammatory disease, is driven by the abnormal activation of T helper (Th) 2 cells and barrier dysfunction. We attempted to identify the anti-inflammatory components of SF-7343. Initial screening showed that compounds 1 and 3 inhibited the secretion of interleukin-8 and -6 in tumor necrosis factor-α/interferon-γ-treated HaCaT cells, and these compounds also showed inhibitory effects on CCL5 and CCL22. Compounds 1 and 3 also downregulated the protein expression levels of intercellular adhesion molecule-1 and upregulated the expression of filaggrin and involcurin. The mechanism study results showed that compounds 1 and 3 inhibited nuclear translocation of nuclear factor-kappa B p65 and the phosphorylation of STAT1 and STAT3. Compound 1, but not compound 3, significantly promoted the expression of heme oxygenase (HO)-1. The effects of compound 1 were partly reversed by co-treatment with a HO-1 inhibitor, tin protoporphyrin IX. Taken together, this study demonstrates the potential value of Antarctic fungal strain SF-7343 isolates as a bioresource for bioactive compounds to prevent skin inflammation.
The Impact of Hypoxia in Early Pregnancy on Placental Cells
Authors: Zhao Hui , Wong Ronald J. , Stevenson David K.
Published: September 2021

Oxygen levels in the placental microenvironment throughout gestation are not constant, with severe hypoxic conditions present during the first trimester. This hypoxic phase overlaps with the most critical stages of placental development, i.e., blastocyst implantation, cytotrophoblast invasion, and spiral artery remodeling initiation. Dysregulation of any of these steps in early gestation can result in pregnancy loss and/or adverse pregnancy outcomes. Hypoxia has been shown to regulate not only the self-renewal, proliferation, and differentiation of trophoblast stem cells and progenitor cells, but also the recruitment, phenotype, and function of maternal immune cells. In this review, we will summarize how oxygen levels in early placental development determine the survival, fate, and function of several important cell types, e.g., trophoblast stem cells, extravillous trophoblasts, syncytiotrophoblasts, uterine natural killer cells, Hofbauer cells, and decidual macrophages. We will also discuss the cellular mechanisms used to cope with low oxygen tensions, such as the induction of hypoxia-inducible factor (HIF) or mammalian target of rapamycin (mTOR) signals, regulation of the metabolic pathway, and adaptation to autophagy. Understanding the beneficial roles of hypoxia in early placental development will provide insights into the root cause(s) of some pregnancy disorders, such as spontaneous abortion, preeclampsia, and intrauterine growth restriction.
Novel Pyridine Bioisostere of Cabozantinib as a Potent c-Met Kinase Inhibitor: Synthesis and Anti-Tumor Activity against Hepatocellular Carcinoma
Authors: Karmacharya Ujjwala , Guragain Diwakar , Chaudhary Prakash , Jee Jun-Goo , Kim Jung-Ae , Jeong Byeong-Seon
Published: September 2021

Two novel bioisosteres of cabozantinib, 3 and 4, were designed and synthesized. The benzene ring in the center of the cabozantinib structure was replaced by trimethylpyridine (3) and pyridine (4), respectively. Surprisingly, the two compounds showed extremely contrasting mesenchymal–epithelial transition factor (c-Met) inhibitory activities at 1 μM concentration (4% inhibition of 3 vs. 94% inhibition of 4). The IC50 value of compound 4 was 4.9 nM, similar to that of cabozantinib (5.4 nM). A ligand-based docking study suggested that 4 includes the preferred conformation for the binding to c-Met in the conformational ensemble, but 3 does not. The anti-proliferative activity of compound 4 against hepatocellular carcinoma (Hep3B and Huh7) and non-small-cell lung cancer (A549 and H1299) cell lines was better than that of cabozantinib, whereas 3 did not show a significant anti-proliferative activity. Moreover, the tumor selectivity of compound 4 toward hepatocellular carcinoma cell lines was higher than that of cabozantinib. In the xenograft chick tumor model, compound 4 inhibited Hep3B tumor growth to a much greater extent than cabozantinib. The present study suggests that compound 4 may be a good therapeutic candidate against hepatocellular carcinoma.
Current View on the Mechanisms of Alcohol-Mediated Toxicity
Authors: Birková Anna , Hubková Beáta , Čižmárová Beáta , Bolerázska Beáta
Published: September 2021

Alcohol is a psychoactive substance that is widely used and, unfortunately, often abused. In addition to acute effects such as intoxication, it may cause many chronic pathological conditions. Some of the effects are very well described and explained, but there are still gaps in the explanation of empirically co-founded dysfunction in many alcohol-related conditions. This work focuses on reviewing actual knowledge about the toxic effects of ethanol and its degradation products.
Oxidative Stress, Mutations and Chromosomal Aberrations Induced by In Vitro and In Vivo Exposure to Furan
Authors: Russo Maria Teresa , De Luca Gabriele , Palma Nieves , Leopardi Paola , Degan Paolo , Cinelli Serena , Pepe Gaetano , Mosesso Pasquale , Di Carlo Emma , Sorrentino Carlo , Musiani Piero , Crebelli Riccardo , Bignami Margherita , Dogliotti Eugenia
Published: September 2021

Furan is a volatile compound that is formed in foods during thermal processing. It is classified as a possible human carcinogen by international authorities based on sufficient evidence of carcinogenicity from studies in experimental animals. Although a vast number of studies both in vitro and in vivo have been performed to investigate furan genotoxicity, the results are inconsistent, and its carcinogenic mode of action remains to be clarified. Here, we address the mutagenic and clastogenic activity of furan and its prime reactive metabolite cis-2 butene-1,4-dial (BDA) in mammalian cells in culture and in mouse animal models in a search for DNA lesions responsible of these effects. To this aim, Fanconi anemia-derived human cell lines defective in the repair of DNA inter-strand crosslinks (ICLs) and Ogg1−/− mice defective in the removal of 8-hydroxyguanine from DNA, were used. We show that both furan and BDA present a weak (if any) mutagenic activity but are clear inducers of clastogenic damage. ICLs are strongly indicated as key lesions for chromosomal damage whereas oxidized base lesions are unlikely to play a critical role.
Apolipoprotein E4 Is Associated with Right Ventricular Dysfunction in Dilated Cardiomyopathy—An Animal and In-Human Comparative Study
Authors: Diaconu Rodica , Schaaps Nicole , Afify Mamdouh , Boor Peter , Cornelissen Anne , Florescu Roberta A. , Simsekyilmaz Sakine , El-Khoury Teddy , Schumacher David , Ioana Mihai , Streata Ioana , Militaru Constantin , Donoiu Ionut , Vogt Felix , Liehn Elisa A.
Published: September 2021

ApoE abnormality represents a well-known risk factor for cardiovascular diseases. Beyond its role in lipid metabolism, novel studies demonstrate a complex involvement of apoE in membrane homeostasis and signaling as well as in nuclear transcription. Due to the large spread of apoE isoforms in the human population, there is a need to understand the apoE’s role in pathological processes. Our study aims to dissect the involvement of apoE in heart failure. We showed that apoE-deficient rats present multiple organ damages (kidney, liver, lung and spleen) besides the known predisposition for obesity and affected lipid metabolism (two-fold increase in tissular damages in liver and one-fold increase in kidney, lung and spleen). Heart tissue also showed significant morphological changes in apoE−/− rats, mostly after a high-fat diet. Interestingly, the right ventricle of apoE−/− rats fed a high-fat diet showed more damage and affected collagen content (~60% less total collagen content and double increase in collagen1/collagen3 ratio) compared with the left ventricle (no significant differences in total collagen content or collagen1/collagen3 ratio). In patients, we were able to find a correlation between the presence of ε4 allele and cardiomyopathy (χ2 = 10.244; p = 0.001), but also with right ventricle dysfunction with decreased TAPSE (15.3 ± 2.63 mm in ε4-allele-presenting patients vs. 19.8 ± 3.58 mm if the ε4 allele is absent, p < 0.0001*) and increased in systolic pulmonary artery pressure (50.44 ± 16.47 mmHg in ε4-allele-presenting patients vs. 40.68 ± 15.94 mmHg if the ε4 allele is absent, p = 0.0019). Our results confirm that the presence of the ε4 allele is a lipid-metabolism-independent risk factor for heart failure. Moreover, we show for the first time that the presence of the ε4 allele is associated with right ventricle dysfunction, implying different regulatory mechanisms of fibroblasts and the extracellular matrix in both ventricles. This is essential to be considered and thoroughly investigated before the design of therapeutical strategies for patients with heart failure.
On the Common Journey of Neural Cells through Ischemic Brain Injury and Alzheimer’s Disease
Authors: Kriska Jan , Hermanova Zuzana , Knotek Tomas , Tureckova Jana , Anderova Miroslava
Published: September 2021

Ischemic brain injury and Alzheimer’s disease (AD) both lead to cell death in the central nervous system (CNS) and thus negatively affect particularly the elderly population. Due to the lack of a definitive cure for brain ischemia and AD, it is advisable to carefully study, compare, and contrast the mechanisms that trigger, and are involved in, both neuropathologies. A deeper understanding of these mechanisms may help ameliorate, or even prevent, the destructive effects of neurodegenerative disorders. In this review, we deal with ischemic damage and AD, with the main emphasis on the common properties of these CNS disorders. Importantly, we discuss the Wnt signaling pathway as a significant factor in the cell fate determination and cell survival in the diseased adult CNS. Finally, we summarize the interesting findings that may improve or complement the current sparse and insufficient treatments for brain ischemia and AD, and we delineate prospective directions in regenerative medicine.
Relationship between Perceived UX Design Attributes and Persuasive Features: A Case Study of Fitness App
Authors: Oyibo Kiemute , Vassileva Julita
Published: September 2021

Research shows that a well-designed user interface is more likely to be persuasive than a poorly designed one. However, there is a limited understanding of the relationship between user-experience (UX) design attributes and users’ receptiveness to the persuasive features of a persuasive technology aimed at motivating behavior change. To bridge this gap, we carried out an online case study among 228 participants from Canada and the United States to investigate the relationship between perceived UX design attributes and users’ receptiveness to persuasive features. The study serves as exploratory work by focusing on a single prototype (homepage of a fitness app); four commonly researched UX design attributes (perceived aesthetics, perceived usability, perceived credibility, and perceived usefulness); and six commonly employed persuasive features (Goal-Setting/Self-Monitoring, Reward, Cooperation, Competition, Social Comparison, and Social Learning) illustrated on storyboards. The results of the Partial Least Square Path Modeling show that perceived usefulness, followed by perceived aesthetics, has the strongest relationship with users’ receptiveness to the persuasive features of a fitness app. Specifically, perceived usefulness and perceived aesthetics have a significant relationship with users’ receptiveness to all but two of the six persuasive features, respectively, as well as with the overall perceived persuasiveness of the fitness app. These findings are supported by participants’ comments on the perceived UX design attributes of the fitness app and the persuasive features illustrated on the storyboards. However, perceived usability and perceived credibility have weak or non-significant relationships with users’ receptiveness to the six persuasive features. The findings suggest that designers should prioritize utilitarian benefits (perceived usefulness) and hedonic benefits (perceived aesthetics) over perceived usability and perceived credibility when designing fitness apps to support behavior change.
UDP-Glucosyltransferases Induced by Nosema bombycis Provide Resistance to Microsporidia in Silkworm (Bombyx mori)
Authors: Yu Bin , Yang Qiuhua , Wei Junhong , Pan Guoqing , Li Chunfeng , Zhou Zeyang
Published: September 2021

As a silkworm pathogen, the microsporidian N. bombycis can be transovarially transmitted from parent to offspring and seriously impedes sericulture industry development. Previous studies found that Uridine diphosphate (UDP)-glycosyltransferases (UGTs) are involved in regulating diverse cellular processes, such as detoxification, pigmentation, and odorant sensing. Our results showed that BmUGT10295 and BmUGT8453 genes were specifically induced in infected silkworms, but other BmUGTs were not. Tissue distribution analysis of the two BmUGTs showed that the transcriptions of the two BmUGTs were mainly activated in the midgut and Malpighian tubule of infected silkworms. Furthermore, there were significantly fewer microsporidia in over-expressed BmUGTs compared with the control, but there were significantly more microsporidia in RNA interference BmUGTs compared with the control. These findings indicate that the two BmUGTs were induced by N. bombycis and provided resistance to the microsporidia.
Effect of Feed Containing Hermetia illucens Larvae Immunized by Lactobacillus plantarum Injection on the Growth and Immunity of Rainbow Trout (Oncorhynchus mykiss)
Authors: Hwang Dooseon , Lim Chae-Hwan , Lee Seung Hun , Goo Tae-Won , Yun Eun-Young
Published: September 2021

We investigated the effects of a feed containing Hermetia illucens larvae injected with bacteria on the growth and immunity of Oncorhynchus mykiss. The feed was prepared by replacing fishmeal in feed with 25 and 50% nonimmunized (HIL25, HIL50) or immunized HIL (ImHIL25, ImHIL50), and its protein:fat:carbohydrate ratio was 45:15:18. ImHIL extracts showed inhibitory activity against fish pathogenic bacteria. Both red blood cell count and insulin-like growth factor-1 as the growth indicator were the highest among the groups at week 6 after feeding in the ImHIL50 group. As immune indicators, blood aspartate aminotransferase levels were lower in the ImHIL25 and ImHIL50 groups than in that of other groups at week 6 after feeding, and lysozyme content was significantly higher in ImHIL25 and ImHIL50. The above results demonstrate that ImHIL has a beneficial effect on the improvement of growth and immunity. Accordingly, we suggest that ImHIL has the potential to be a good feed source in aquaculture.
Self-Propelled Aero-GaN Based Liquid Marbles Exhibiting Pulsed Rotation on the Water Surface
Authors: Braniste Tudor , Ciobanu Vladimir , Schütt Fabian , Mimura Hidenori , Raevschi Simion , Adelung Rainer , Pugno Nicola M. , Tiginyanu Ion
Published: September 2021

We report on self-propelled rotating liquid marbles fabricated using droplets of alcoholic solution encapsulated in hollow microtetrapods of GaN with hydrophilic free ends of their arms and hydrophobic lateral walls. Apart from stationary rotation, elongated-spheroid-like liquid marbles were found, for the first time, to exhibit pulsed rotation on water surfaces characterized by a threshold speed of rotation, which increased with the weight of the liquid marble while the frequency of pulses proved to decrease. To throw light upon the unusual behavior of the developed self-propelled liquid marbles, we propose a model which takes into account skimming of the liquid marbles over the water surface similar to that inherent to flying water lily beetle and the so-called helicopter effect, causing a liquid marble to rise above the level of the water surface when rotating.
Estimation of Post-Cracking Dissipation Capabilities of Fiber Reinforced Concretes in Three Point Bending Test Monitored with Application of Digital Image Correlation System
Authors: Trinh-Duc Duyen , Piotrowski Andrzej , Ajdukiewicz Cezary , Woyciechowski Piotr , Gajewski Marcin
Published: September 2021

Concretes with dispersed reinforcement are increasingly used in structural engineering. The basic source of knowledge on their application and design are the Model-Code 2010 guidelines. These guidelines, however, apply mainly to steel rebar reinforcement and are not fully sufficient in the analysis of the load-bearing capacity of elements made of concrete with dispersed reinforcement. Therefore, scientific research in this field is carried out continuously. The main goal of our work is to provide experimental data for the calibration of constitutive models of the cracking mechanics of concrete with reinforcement in the form of steel and polypropylene fibers. This article shows the possibility of using the digital image correlation system (DIC) to achieve this goal. The method of sample preparation and the method of conducting the tests were modeled on the recommendations contained in the PN-EN 14651: 2007 standard. The tests were carried out on prismatic elements with a notch loaded in a three-point bending setup. The results of standard strength tests are presented in the form of column graphs and tables. As an extension, the results of calculations of energy dissipated in fracture process are given. Moreover, the experimentally obtained graphs of the relationship between the force, displacement and crack opening were presented, which were supplemented with the images of crack development obtained with the use of DIC. The development of the crack net is characterized not only qualitatively but also quantitatively as a function of deflection or crack mouth opening displacement. Conclusions concerning the adopted research methodology and the tested materials are presented at the end of the article.
Rheological Performance of Magnetorheological Grease with Embedded Graphite Additives
Authors: Mohd Nasir Nur Alyaa , Nazmi Nurhazimah , Mohamad Norzilawati , Ubaidillah Ubaidillah , Nordin Nur Azmah , Mazlan Saiful Amri , Abdul Aziz Siti Aishah , Shabdin Muhammad Kashfi , Yunus Nurul Azhani
Published: September 2021

The use of highly viscous grease as a medium in magnetorheological grease (MRG) provides the benefit of avoiding sedimentation from occurring. However, it limits the expansion of yield stress in the on-state condition, thus reducing the application performance during operation. Therefore, in this study, the improvement in the rheological properties of MRG was investigated through the introduction of graphite as an additive. MRG with 10 wt % graphite (GMRG) was fabricated, and its properties were compared to a reference MRG sample. The microstructure of GMRG was characterized using an environmental scanning electron microscope (ESEM). The rheological properties of both samples, including apparent viscosity, yield stress, and viscoelasticity, were examined using a shear rheometer in rotational and oscillatory modes. The results demonstrated a slight increase in the apparent viscosity in GMRG and a significant improvement in yield stress by 38.8% at 3 A with growth about 32.7% higher compared to MRG from 0 to 3 A. An expansion of the linear viscoelastic region (LVE) from 0.01% to 0.1% was observed for the GMRG, credited to the domination of the elastic properties on the sample. These obtained results were confirmed based on ESEM, which described the contribution of graphite to constructing a more stable chain structure in the GMRG. In conclusion, the findings highlight the influence of the addition of graphite on improving the rheological properties of MRG. Hence, the addition of graphite in MRG shows the potential to be applied in many applications in the near future.
Influence of Spin Coating Parameters on Gas Transport Properties of Thin-Film Composite Membranes
Authors: Sokolov Stepan , Balynin Alexey , Bakhtin Danila , Borisov Ilya
Published: September 2021

The influence of casting centrifugation process parameters, such as a rotation speed (ω), the amount of the film-forming solution (V), and its concentration (C) on transport properties of composite membranes were investigated. A number of composite membranes based on poly (1-trimethylsilylpropyne) (PTMSP) and micro- (MFFK-1) and ultrafiltration (UFFK) membranes were obtained using the spin-coating method. For the first time, an unexpected dependence of permeance and ideal selectivity on rotation speed had been discovered: the thickness of the selective layer decreases from 3.0 to 1.0 μm for MFFK-1 and from 1.7 to 1.1 μm for UFFK with an increase of spin coater rotation speed from 500 to 3000 rpm. However, the gas permeance of composite membranes in the range of 500–2000 rpm was reduced due to an increase of a penetration depth of PTMSP into a support layer porous structure (estimated by the EDX method). The permeance of the PTMSP/UFFK membranes was higher than PTMSP/MFFK-1 membranes due to a thinner selective layer and a lower penetration depth of polymer solution into the pores of the support. The highest CO2/N2 selectivity values were achieved as 5.65 ± 0.9 at CO2 permeance 5600 ± 1000 GPU for PTMSP/UFFK membranes (CPTMSP = 0.35%, Vsolution = 1 mL, ω = 1000 rpm), and 6.1 ± 0.5 at CO2 permeance 4090 ± 500 GPU for PTMSP/MFFK-1 membranes (CPTMSP = 0.35%, Vsolution = 1 mL, ω = 2000 rpm).
Optimizing the Mechanical Properties of Ultra-High-Performance Fibre-Reinforced Concrete to Increase Its Resistance to Projectile Impact
Authors: Mina Anna L. , Trezos Konstantinos G. , Petrou Michael F.
Published: September 2021

This study describes an extensive experimental investigation of various mechanical properties of Ultra-High-Performance Fibre-Reinforced Concrete (UHPFRC). The scope is to achieve high strength and ductile behaviour, hence providing optimal resistance to projectile impact. Eight different mixtures were produced and tested, three mixtures of Ultra-High-Performance Concrete (UHPC) and five mixtures of UHPFRC, by changing the amount and length of the steel fibres, the quantity of the superplasticizer, and the water to binder (w/b) ratio. Full stress–strain curves from compression, direct tension, and flexural tests were obtained from one batch of each mixture to examine the influence of the above parameters on the mechanical properties. The Poisson’s ratio and modulus of elasticity in compression and direct tension were measured. Additionally, a factor was determined to convert the cubic strength to cylindrical. Based on the test results, the mixture with high volume (6%) and a combination of two lengths of steel fibres (3% each), water to binder ratio of 0.16% and 6.1% of superplasticizer to binder ratio exhibited the highest strength and presented great deformability in the plastic region. A numerical simulation developed using ABAQUS was capable of capturing very well the experimental three-point bending response of the UHPFRC best-performed mixture.
Experimental and Analytical Analysis of Mechanical Properties for Large-Size Lattice Truss Panel Structure Including Role of Connected Structure
Authors: Li Shaohua , Jiang Wenchun , Zhu Xiaolei , Xie Xuefang
Published: September 2021

The large-size lattice truss panel structure (LTPS) is continually increasing for higher upsizing, but the roles of its connected structures on the mechanical properties are always ignored during the previous structural integrity assessment. Thus, in this paper, a series of mechanical tests, including the fabricating of panel-to-panel LTPSs, monotonous tensile, and three- and four-point bending tests, were performed to comprehensively understand the mechanical behavior. Furthermore, a theoretical model including the role of connected structures was developed to predict both the elastic and plastic deformation behavior of panel-to-panel LTPS. Results show that the connected structure has a very significant effect on the mechanical properties of panel-to-panel LTPS during the three-bending tests, and I-beam element depresses its carrying capacity. The developed theoretical model was proved to accurately predict the experimental results, and the maximum error was limited within 20%. Finally, the dimensional effects of the connection components on mechanical properties were also analyzed by the theoretical model, and indicated that the panel-to-panel LTPS will present better mechanical performance than the intact structure when the width of I-beam element exceeds 12.2 mm or the its length downgrades to 39.1 mm, which provide a comprehensive guidance for the engineering design of large-size LTPS.
Performance of MoS2/Zr Composite Coatings at Different Deposition Temperatures
Authors: Song Wenlong , Sun Kai , Zhao Guangming , Zhu Long , Wang Shoujun , Li Tianya
Published: September 2021

The properties of the MoS2/Zr coatings can be significantly affected by the deposition temperature. In this study, the MoS2/Zr composite coatings were fabricated on the cemented carbide surface, utilizing the duplex deposition technology at various deposition temperatures. The effects of deposition temperature on the mechanical and friction properties of the MoS2/Zr coatings were systematically studied. Results exhibited that as the deposition temperature increased, the adhesion force increased first and then decreased, and the coating thickness and micro-hardness gradually increased. Dry sliding tests against a hardened steel ring showed that the tribological behaviors and wear mechanisms of the MoS2/Zr coatings varied with deposition temperature, which were due to the changing mechanical properties of coatings caused by the temperature. The coatings deposited at a temperature of 180 °C and 200 °C possessed preferable comprehensive mechanical and tribological properties.
Microstructure Evolution in He-Implanted Si at 600 °C Followed by 1000 °C Annealing
Authors: Yang Zhen , Zou Zhiping , Zhang Zeyang , Xing Yubo , Wang Tao
Published: September 2021

Si single crystal was implanted with 230 keV He+ ions to a fluence of 5 × 1016/cm2 at 600 °C. The structural defects in Si implanted with He at 600 °C and then annealed at 1000 °C were investigated by transmission electron microscopy (TEM) and high-resolution transmission electron microscopy (HRTEM). The microstructure of an as-implanted sample is provided for comparison. After annealing, rod-like defects were diminished, while tangled dislocations and large dislocation loops appeared. Dislocation lines trapped by cavities were directly observed. The cavities remained stable except for a transition of shape, from octahedron to tetrakaidecahedron. Stacking-fault tetrahedrons were found simultaneously. Cavity growth was independent of dislocations. The evolution of observed lattice defects is discussed.
Comparison of NSGA-II, MOALO and MODA for Multi-Objective Optimization of Micro-Machining Processes
Authors: Joshi Milan , Ghadai Ranjan Kumar , Madhu S. , Kalita Kanak , Gao Xiao-Zhi
Published: September 2021

The popularity of micro-machining is rapidly increasing due to the growing demands for miniature products. Among different micro-machining approaches, micro-turning and micro-milling are widely used in the manufacturing industry. The various cutting parameters of micro-turning and micro-milling has a significant effect on the machining performance. Thus, it is essential that the cutting parameters are optimized to obtain the most from the machining process. However, it is often seen that many machining objectives have conflicting parameter settings. For example, generally, a high material removal rate (MRR) is accompanied by high surface roughness (SR). In this paper, metaheuristic multi-objective optimization algorithms are utilized to generate Pareto optimal solutions for micro-turning and micro-milling applications. A comparative study is carried out to assess the performance of non-dominated sorting genetic algorithm II (NSGA-II), multi-objective ant lion optimization (MOALO) and multi-objective dragonfly optimization (MODA) in micro-machining applications. The complex proportional assessment (COPRAS) method is used to compare the NSGA-II, MOALO and MODA generated Pareto solutions.
Experimental Investigations on Electric-Field-Induced Crystallization in Erythritol
Authors: Dauvergne Jean-Luc , Nikulin Artem , Doppiu Stefania , Palomo del Barrio Elena
Published: September 2021

The objective of this experimental study was to develop a method to induce crystallization of sugar alcohols using an electric field for its future implementation in latent heat thermal energy storage systems. To better understand the mechanisms behind this approach, the first step of this work was dedicated to the replication, continuation, and consolidation of promising results on erythritol reported by another research group. In the second step, a second experimental configuration, previously used to electrically control the supercooling of other phase change materials, was tested with the same sugar alcohol. For both configurations, the influence of the type of current (DC and AC at different frequencies), its amplitude, and time of exposure were studied. However, none of these tests allowed influencing the crystallization of erythritol. Even if surprising at first glance, the difficulty in reproducing experiments and interpreting the results is not new in the field of electric-field-induced crystallization, as shown in particular by the abundant literature reviews concerning water. Currently, to the best of our knowledge, we consider that electric fields could be an attractive option to initiate and accelerate the crystallization of erythritol, but this solution must be considered with caution.
Position Control and Force Estimation Method for Surgical Forceps Using SMA Actuators and Sensors
Authors: Braun Dennis , Weik David , Elsner Sophia , Hunger Sandra , Werner Michael , Drossel Welf-Guntram
Published: September 2021

Minimally invasive surgery is increasingly used in many medical operations because of the benefits for the patients. However, for the surgeons, accessing the situs through a small incision or natural orifice comes with a reduction of the degrees of freedom of the instrument. Due to friction of the mechanical coupling, the haptic feedback lacks sensitivity that could lead to damage of the tissue. The approach of this work to overcome these problems is to develop a control concept for position control and force estimation with shape memory alloys (SMA) which could offer haptic feedback in a novel handheld instrument. The concept aims to bridge the gap between manually actuated laparoscopic instruments and surgical robots. Nickel-titanium shape memory alloys are used for actuation because of their high specific energy density. The work includes the manufacturing of a functional model as a proof of concept comprising the development of a suitable forceps mechanism and electronic circuit for position control and gripping force measurement, as well as designing an ergonomic user interface with haptic force feedback.