bluuebunny/crossref_metadata_2025 huggingface dataset:
Trajetória do CadÚnico durante o ciclo petista
Authors: Farias Luciana de
Published: October 2020

O Cadastro Único para Programas Sociais (CadÚnico) foi criado em 2001, ainda sob a gestão federal de Fernando Henrique Cardoso (PSDB), mas ele se consolidou durante os governos petistas de Luís Inácio Lula da Silva (2003-2010) e Dilma Vana Rousseff (2011-2016). O objetivo deste artigo é descrever os atores e os arranjos institucionais que consolidaram e expandiram o CadÚnico ao longo dos anos 2003 a 2016 investigando as escolhas e as relações imbricadas no seu funcionamento, sua constante construção e seu caráter gerador de novos marcos legais e institucionais das políticas sociais. A trajetória narrada no artigo foi construída com base na revisão bibliográfica sobre programas sociais e sobre o CadÚnico, entrevistas e análise documental. O artigo conclui que o CadÚnico é um objeto vivo e em transformação, seu ambicioso objetivo de ser a única ferramenta de cadastramento para os beneficiários de programas sociais foi alcançado paulatinamente e contou com a participação de atores e de instituições diversos através da construção de relações complexas.
expansão do ensino superior na Amazônia brasileira
Authors: Silva Jairo da Silva e
Published: October 2020

Esse artigo tem por objetivo comparar a expansão do ensino superior durante os governos presidenciais de Fernando Henrique Cardoso (1995-1998, 1999-2002) e o ciclo petista (2003-2016), principalmente na Amazônia brasileira. Para tanto, adotou-se como procedimentos metodológicos a abordagem qualitativa/interpretativista, a partir de reflexões apresentadas por estudiosos da educação brasileira e dados oficiais, permitindo-nos analisar as dinâmicas da expansão dessa modalidade de ensino no Brasil nas duas últimas décadas. Os resultados revelam que, quando estes ciclos presidenciais são comparados, o ensino superior brasileiro no aspecto quantiqualitativo, tanto público, quanto privado, a expansão é mais evidente nos governos do Partido dos Trabalhadores.
ação sindical petroleira nos governos do partido dos trabalhadores (2003-2016)
Authors: Mendes Danilo Lucena
Published: October 2020

O presente artigo oferece uma visada sobre a ação da Federação Única dos Petroleiros (FUP) durante os governos do Partido dos Trabalhadores (PT). Com base no recente debate sobre o comportamento do sindicalismo ao longo desses anos, e a observação do posicionamento político dos petroleiros na política de desenvolvimento econômico-social dos governos dos dois presidentes do PT, pretendemos ampliar a compreensão acerca do elo entre os governos petistas e trabalhadores nos anos 2000.
Discurso de ódio acerca do jovem
Authors: Accorssi Aline , Netto Livian Lino , Clase Julia Rocha
Published: October 2020

O presente artigo procura refletir sobre as representações sociais de internautas, a partir dos seus comentários em uma reportagem de um jornal local, sobre a ocupação estudantil secundarista no Instituto Federal Sul-rio-grandense (Campus Pelotas). Foram analisados os 271 comentários realizados na matéria sobre a ocupação, publicada no dia 16 de outubro de 2016, na página do jornal no Facebook. Os dados foram submetidos a uma análise de conteúdo do tipo temática, o que gerou um mapa representacional. O tema de maior prevalência e que perpassa todos os demais, sendo caracterizador da representação social de juventude, está relacionado a desqualificação juvenil. Dele derivam outros dois grupos de pensamentos acerca do jovem: o primeiro refere-se à imaturidade, incapacidade e improdutividade, já o segundo à fácil manipulação frente aos modismos e suposta doutrinação da esquerda. Diante disso, cabe à sociedade a condução da construção social do jovem enquanto “homem de bem”. O desvio dessa formação caracterizaria uma inversão da ordem, uma contravenção, a qual necessita de controle, punição e ódio.
matrimônio segundo Kierkegaard frente ao amor líquido de Zygmunt Bauman
Authors: Oliveira Wanderley Costa de
Published: October 2020

O conceito de matrimônio de Søren Kierkegaard (1813-1855) está expresso nos Stadier, que tem uma parte traduzida ao português como “O matrimônio”. Tomamos como ponto de partida que a reflexão do autor ainda pode ser pertinente ao contexto da Modernidade contemporânea, especialmente em diálogo com o conceito de “Amor Líquido”, presente na obra homônima do sociólogo polonês Zygmunt Bauman (1925-2017). O matrimônio, na visão de Kierkegaard, é a união de espírito em que os amantes celebram o amor, e para mantê-lo longevo, a manutenção do amor erótico é necessária, uma vez que o amor precede o matrimônio. Por outro lado, no “amor líquido”, a instabilidade, a fugacidade e o consumismo impedem a realização do matrimônio, flexibilizando-o a modos instantâneos e insensíveis à afetividade. A hipótese deste artigo é a de que uma convergência entre os autores está na condição que a vida conjugal é entendida: nada é estável existencialmente que possa durar para sempre nem antes, nem depois do matrimônio. Considerando a ligação que os conceitos de Kierkegaard e de Bauman podem ter com a perspectiva existencialista na Idade Contemporânea, poderemos observar como os laços afetivos foram abalados sensivelmente no século XX.
Desvendando relações
Authors: Azevedo Leonardo Francisco de
Published: October 2020

Compreender a experiência humana para além da dicotomia agência-estrutura é um desafio encarado por diferentes teorias e escolas antropológicas. Nesse trabalho apresentaremos três diferentes teorias que buscaram superar a explicação estruturalista do mundo: os estudos de redes sociais, propostos pela antropologia britânica na segunda metade do século XX – nomeadamente a “Escola de Manchester”, como forma de superar o estrutural-funcionalismo; a Teoria ator-rede proposta pelo francês Bruno Latour, como forma de superar a “sociologia do social”; e a noção de malha, elaborada por Tim Ingold na construção de uma “antropologia da vida”. Todas as três perspectivas, de alguma maneira, recorrem ao conceito de “rede”. Entretanto, tal presença tem sentidos radicalmente diferentes em cada uma delas.
uso de documentación folclórica en Argentina y Brasil para comprender las prácticas de curación
Authors: Almeida Diádiney Helena de , Dahhur Astrid
Published: October 2020

El siguiente trabajo es el enfoque de un problema compartido por intelectuales brasileros y argentinos entre finales del siglo XIX y mediados del siglo XX. A través de textos folclóricos analizamos la construcción intelectual de la llamada “medicina popular” como un campo de estudio por parte de investigadores de ambos países. Presentamos así el desarrollo de ambos casos prestando atención a cómo lo “popular” fue analizado en un contexto de expansión de los nacionalismos como así también de fortalecimiento de la disciplina folclórica. Se trata de llamar la atención sobre una amplia documentación histórica que requiere un análisis crítico para presentar las diferentes concepciones de curación y de curadores brasileros y argentinos.
pesquisa em HIV/aids nas ciências sociais
Authors: Oliveira Kris Herik de
Published: October 2020

As contribuições das Ciências Sociais diante da epidemia de HIV/aids têm sido pouco examinadas no Brasil em termos quantitativos e qualitativos. Para experimentar aproximações junto ao “estado da arte” deste campo de pesquisas, o presente trabalho pretendeu mapear e analisar as teses e dissertações brasileiras em HIV/aids na área de Ciências Sociais. Através do levantamento bibliográfico no Catálogo de Teses e Dissertações da CAPES foram identificadas 111 dissertações e 48 teses, abrangendo o período de 1990 a 2018. Análises de estatística descritiva e de conteúdo foram empregadas e discutidas acerca do volume, área do conhecimento, período, instituição, região, gênero, temas e metodologias. Dentre os resultados, destacam-se: o crescimento expressivo de estudos e o processo de diversificação do material empírico ao longo dos anos; as mulheres como as principais interlocutoras; práticas terapêuticas e de cuidado como temas menos frequentes; a carência de estudos junto à população negra, aos indígenas e às expressões de gênero e sexualidade em sua pluralidade; as instituições públicas de saúde, empresas e laboratórios como contextos pouco ou ainda não explorados. Ademais, o artigo atenta para as possibilidades de expansão de pesquisas com as novas biotecnologias e mídias digitais.
Quando o raro se torna comum
Authors: Souza Bruna Aline Stoél de , Carniel Fagner
Published: October 2020

O artigo aborda a história de uma mãe que vivenciou a experiência de ter um filho com adrenoleucodistrofia em meio ao processo de emergência de uma institucionalidade para o agenciamento de pessoas com doenças raras no Brasil. O desenho de pesquisa parte da forma pela qual as doenças raras se constituíram enquanto um problema de gestão pública em âmbito nacional e internacional. Em seguida, por meio de fontes documentais, dialoga com os relatos de Lindacir Souza Franco a respeito do adoecimento, da busca por um diagnóstico conclusivo e por tratamentos possíveis até a morte de seu filho Gabriel, em fevereiro de 2017. Por fim, discute-se as maneiras pelas quais Lindacir conseguiu ressignificar sua “tragédia pessoal” em um estilo de militância que visa transformar a “raridade” da doença de seu filho em algo cada vez mais “comum” para construir alianças com associações e profissionais de saúde que a desconhecem. A intenção, portanto, não é a de realizar uma genealogia da doença a partir da postulação de novas alteridades identificadas como “raras”, mas oferecer elementos empíricos que ampliem o debate acerca dos desafios implicados nas lutas por reconhecimento e por direitos à saúde.
Cuando lo esencial se hace visible
Authors: Testa Daniela
Published: October 2020

El texto comparte reflexiones provisorias sobre el impacto de la pandemia de COVID19en Argentina. Se describen medidas sanitarias y de protección social adoptadas por el gobierno nacional y sus vinculaciones con la capacidad de respuesta del sistema sanitario. Si bien Argentina cuenta con un sector público de salud de carácter universal y gratuito, el mismo arrastra serios problemas de fragmentación y accesibilidad que se magnifican frente a la emergencia. Se propone la idea de “etcétera del cuidado” para visibilizar el carácter micropolítico del trabajo en salud y el relevante papel de las mujeres en contextos epidémicos. Asi mismo se analiza el alcance desigual de la pandemia entre los más vulnerables.
PERBANDINGAN EMISI GAS BUANG YANG DIHASILKAN PADA PENERAPAN BIODIESEL DI PT ADARO INDONESIA
Authors: Firdausy Muhammad Abrar , Mizwar Andy , Khair Riza Miftahul , Nirtha Rd. Indah , Hamatha Nadieda
Published: October 2020

Penelitian ini bertujuan untuk mengidentifikasi emisi gas buang oksida nitrogen (NOx) dan partikulat yang dihasilkan oleh biodiesel serta menganalisis pengaruh biodiesel terhadap emisi gas buang yang dihasilkan genset di PT Adaro Indonesia. Metode analisis yang digunakan adalah Analysis of Variance (ANOVA). Variabel bebas berupa variasi bahan bakar, yaitu solar (B0) dan biodiesel dengan persentase 8,75%, 10,55%, 17,5%, dan 26,66%. Variabel terikat berupa parameter uji NOx dan partikulat. Nilai emisi gas buang tertinggi pada emisi NOx dihasilkan oleh B17,5 yaitu 995 mg/Nm3, sedangkan nilai terendah dihasilkan oleh B0, yaitu 121, 838 mg/Nm3. Nilai emisi partikulat tertinggi dihasilkan oleh B0 yaitu 91,572 mg/Nm3, sedangkan yang terendah dihasilkan B10,55, yaitu 15,5 mg/Nm3. Hasil analisis ANOVA menunjukkan bahwa terdapat pengaruh signifikan antara variasi bahan bakar terhadap nilai emisi NOx dengan nilai signifikansi berturut-turut 0,005, sedangkan partikulat tidak memiliki pengaruh signifikan antara variasi bahan bakar terhadap hasil emisi dengan nilai siginfikansi 0,218. Kata kunci: Biodiesel, emisi, gas buang.  This study aims to identify nitrogen oxide (NOx) and particulate emissions produced by biodiesel and analyze the effect of biodiesel on exhaust emissions produced by genset at PT Adaro Indonesia. The analytical method used is Analysis of Variance (ANOVA). The independent variable is fuel variation, namely diesel fuel (B0) and biodiesel with a percentage of 8.75%, 10.55%, 17.5%, and 26.66%. The dependent variable is the test parameters of NOx and particulate. The highest exhaust gas emission in NOx produced by B17.5 are and 995 mg/Nm3, while the lowest value is produced by B0, which is 121, 838 mg/Nm3. The highest particulate emission produced by B0 which is 91.572 mg/Nm3, while the lowest is produced by B10.55, which is 15.5 mg/Nm3. ANOVA analysis results show that there is a significant influence between the fuel variation on the results of NOx gas emissions with significance value of 0.005, while the particulate parameter do not have a significant effect between the fuel variation on the emissions with significance value of 0.218. Keywords: Biodiesel, emission, exhaust gas.
Light Reflected from Different Plant Canopies Affected Beta Vulgaris Growth and Development
Authors: Adjesiwor Albert T. , Kniss Andrew R.
Published: October 2020

Studies on crop response to light quality [red (R) to far-red (FR) light ratio] often recommend early weed removal to reduce the effects of shade avoidance responses on crop yield. However, it is unclear whether crops are able to distinguish reflected light quality of kin from that of non-kin. We evaluated the response of sugarbeet (Beta vulgaris L.) to reflected FR light from sugarbeet, common lambsquarters (Chenopodium album L.), Kentucky bluegrass (Poa pratensis L.), alfalfa (Medicago sativa L.), and bare soil (control) under outdoor conditions in 2016 and 2017. Treatments were completely randomized with 10 replications per treatment. The study methods ensured there was no direct resource competition. The reflected R:FR of plant species ranged from 0.06 (common lambsquarters) to 0.24 (sugarbeet) compared to 0.7 for the bare soil. In both 2016 and 2017, there were 2 to 4 more leaves in the sugarbeet surrounded by soil compared to sugarbeet surrounded by neighboring species. There was up to 47, 57, 43, and 23% reduction in sugarbeet leaf area, shoot dry weight, root diameter, and root dry weight, respectively, due to reflected R:FR light from neighboring species. Sugarbeet did not respond differently to reflected light quality of kin compared to non-kin.
Lactobacillus Rhamnosus GR-1 Ameliorates Trueperella Pyogenes–Induced Barrier Dysfunction of Bovine Endometrial Epithelial Cells
Authors: Liu Ning , Wu Xuan , Wang Mengling , Li Yanan , Xu Le , Zhu Yaohong
Published: October 2020

Trueperella pyogenes is a common opportunistic pathogen which is one of the main causes of postpartum endometritis in dairy cows. As a substitute for antibiotics, the probiotic Lactobacillus rhamnosus GR-1 has been used in a wide range of clinical treatments. Our experiments were designed to establish a model of anti-damage which LGR-1 was used to protect bovine endometrial epithelial cells (BEECs) from inflammatory damage and cell destruction caused by T. pyogenes. Increased expression of NLRP3 inflammasomes and cytokines was observed following T. pyogenes challenge, but this increase was relieved by LGR-1 pretreatment. Immunofluorescence and Western blot analyses revealed that T. pyogenes infection also results in the damage of tight junction proteins in BEECs. The expression levels of Claudin-1, Occludin, and ZO-1 were decreased in cells only infected with T. pyogenes but not in cells pretreated with LGR-1. Moreover, the detection of the anti-apoptotic protein Bcl-2 and apoptotic proteins BAX, cytochrome c, as well as the activating effector caspase-3 revealed that T. pyogenes induced apoptosis of BEECs, which was also confirmed by DAPI staining to observe the morphological changes of the nuclei of cell apoptosis and by TUNEL staining to locate the cells undergoing apoptosis. Our data indicate that LGR-1 ameliorates the T. pyogenes–induced barrier dysfunction of BEECs and pre-application of LGR-1 could be an effective strategy for controlling T. pyogenes infection.
Pengaruh Metode Pembelajaran dan Minat Baca Terhadap Hasil Belajar Sejarah Siswa SMAN 01 Argamakmur Bengkulu Utara
Authors: Al Fiqri Yanuar , Kurniawati Kurniawati , Sarkadi Sarkadi
Published: October 2020

The purpose of this research is to determine whether the teaching method and reading interest  affecting the  historical learning outcome of the High School Student. The sample in this research is the student of SMAN 01 Argamakmur. This research used the quantitative research method with ANAVA 2 x 2. The result indicated: (1) Historical learning outcome of the student who applied the PQ4R method is higher than student who apllied the Direct instruction method. (2) There are interaction between teaching method and reading interest who affected the student hictorical learning outcome. (3) The historical learning outcome of the student who had a high interest in reading  and applied the PQ4R method are higher than student who applied the direct instructional teaching method. (4) The historical learning outcome of the student who had a lowest interest in reading and applied the direct instructional teaching method are higher than student who applied the PQ4R method. In conclusion, teaching method and reading interest affecting the student historical learning outcome of the High School Student.

Penelitian ini bertujuan untuk mengetahui apakah terdapat pengaruh antara metode pembelajaran dan minat baca siswa terhadap hasil belajar sejarah siswa SMA. Sampel dalam penelitian ini adalah siswa SMAN 01 Argamakmur. Penelitian ini menggunakan metode kuantitatif dengan ANAVA 2 x 2. Hasil penelitian menunjukkan: (1) hasil belajar sejarah siswa yang diberikan metode pembelajaran PQ4R lebih tinggi dari siswa yang diberikan metode pembelajaran langsung. (2) terdapat pengaruh interaksi antara metode pembelajaran dan minat baca siswa terhadap hasil belajar sejarah. (3) hasil belajar sejarah siswa yang memiliki minat baca tinggi dan diberikan metode pembelajaran PQ4R, lebih tinggi dari siswa yang diberikan metode pembelajaran langsung. (4) hasil belajar sejarah siswa yang memiliki minat baca yang rendah dan diberikan metode pembelajaran PQ4R lebih rendah dari siswa yang diberikan metode pembelajaran langsung. Kesimpulan dalam penelitian ini adalah, metode pembelajaran dan minat baca siswa mempengaruhi hasil belajar sejarah siswa SMA.
Pengembangan Paket Modul Cetak Mata Pelajaran Pendidikan Agama Islam (PAI) untuk Siswa SMA Negeri 1 Wangi-wangi Kabupaten Wakatobi
Authors: Westomi Joko Azis , Ibrahim Nurdin , Sukardjo Moch
Published: October 2020

The purpose of this research and development is to produce a package of Islamic religious education print modules, to determine the feasibility of a packageof Islamic religious education print modules, and to find out the effectiveness of the Islamic religious education print module package. Modules are used for selfdirected and conventional learning. The research method used is the development method. This study uses the Rowntree development model consisting of three stages of preparation, namely the planning stage, the preparation stage of writing, and the writing and editing stages. Testing the product done to the material experts showed the average score of 4.66 means that the product is considered good, and media experts of 4.79 means the product is considered very good, to students, ie 3 students for the stage face to face with the results of the average score the average of 3.30 means that the product is considered good, and 30 students for the field trial evaluation stage of 4.31 means that the product is considered very good. In conclusion the product can already be said to be good but still there needs to be some improvement in accordance with expert advice, students, and teachers.
Peningkatkan Hasil Belajar Matematika dan Kemampuan Pemecahan Masalah Siswa SMA Melalui Pendekatan Saintifik
Authors: Rahmayanti Ayu , Wibawa Basuki , Khaerudin Khaerudin
Published: October 2020

Penelitian tindakan ini mengangkat tentang Peningkatkan kemampuan pemecahan masalah dan hasil belajar matematika melalui model pembelajaran berbasis masalah.

Penelitian ini dilaksanakan di SMA Negeri 1 Polongbangkeng Utara Kabupaten Takalar, bekerjasama dengan guru mata pejaran untuk memberikan tindakan (proses pembelajaran dengan model pembelajaran berbasis masalah) dan berkolaborasi dengan guru dari sekolah lain yang telah mendapatkan pelatihan K13..Penelitian tindakan ini dilaksanakan pada semester genap, tahun ajaran 2017/2018.Sebelumnya peneliti telah melakukan studi pendahuluan mulai bulan September 2015.

Manfaat penelitian ini adalah dapat menemukan berbagai kendala sekaligus pemecahannya, Secara khusus, untuk: (1) Meningkatkan kemampuan pemecahan masalah siswa melalui pendekatan saintifik dengan model pembelajaran berbasis masalah, (2) Meningkatkan hasil belajar matematika siswa melalui pendekatan saintifik dengan model pembelajaran berbasis masalah.

Hasil penelitian menunjukkan bahwa dengan menggunakan model pembelajaran berbasis masalah   terjadi perubahan pada siswa, yaitu siswa menjadi tertarik, dan aktif serta termotivasi sehingga meningkatkan hasil belajar matematika siswa.
Pengembangan Bahan Ajar Integrasi Nasional Berbasis Mobile
Authors: Nurzaelani Mohammad Muhyidin , Kasman Rusdi , Achyanadia Septy
Published: October 2020

Tujuan penelitian ini yaitu mengembangkan bahan ajar Integrasi Nasional berbasis mobile untuk Mahasiswa Universitas Ibn Khaldun Bogor. Penelitian ini menggunakan metode dengan pendekatan Reserch and Development (R&D). Langkah dalam penelitian ini menggunakan model ADDIE yaitu analisis, desain, pengembangan, implementasi, dan evaluasi. Hasil penelitian menunjukkan bahwa penilaian yang dilakukan oleh tiga orang ahli yaitu ahli media, ahli konten, dan ahli desain pembelajaran mendapatkan hasil sangat layak. Presentase kelayakan bahan ajar integrasi nasional menurut ahli konten sebesar 83,8%, menurut ahli media sebesar 88,9%, dan menurut ahli desain pembelajaran sebesar 89,8%. Secara keseluruhan presentase kelayakan bahan ajar integrasi nasional menurut ahli sebesar 87,5% yang tergolong kategori sangat layak. Selain penilaian oleh ahli, dilakukan juga penilaian oleh 50 orang mahasiswa dengan hasil presentase kelayakan sebesar 94,48% yang tergolong kategori sangat layak.
E-Modul Etnokontruktivisme: Implementasi Pada Kelas V Sekolah Dasar Ditinjau Dari Persepsi, Minat Dan Motivasi
Authors: Syahrial  , Asrial  , Kurniawan Dwi Agus , Piyana Suci Okta
Published: October 2020

Abstrak: Penelitian ini bertujuan untuk mengembangkan media pembelajaran berupa bahan ajar dalam bentuk modul elektronik dengan menggunakan aplikasi software 3D Pageflip Professional untuk siswa kelas V Sekolah Dasar. Metode dalam penelitian ini menggunakan metode Research and Development (R&D) yang mengadaptasi model pengembangan ADDIE (Analysis, Design, Development, Implementation, Evaluation). Berdasarkan hasil penilaian dalam penelitian ini pada angket Persepsi menperoleh frekuensi sebanyak 25 siswa dengan persentase 100% dalam kategori cukup. Untuk angket minat mendapatkan frekuensi sebanyak 20 siswa dengan persentase sebesar 80% dalam kategori baik. Sementara untuk angket motivasi diperoleh frekuensi sebanyak 25 siswa dengan persentase sebesar 100% dalam kategori baik. Dari data yang dihasilkan menunjukkan bahwa modul elektronik etnokontruktivisme berbasis software 3D Pageflip Professional yang dikembangkan dapat digunakan sebagai bahan ajar dalam proses pembelajaran.
Model Perancangan Pembelajaran Blended Mata Kuliah Keilmuan Akuntasi Di ITB-AD Jakarta
Authors: Nurcahyo Widyat , Agustina Yumniati , Efriadi Adi Rizfal
Published: October 2020

This research aims to build a blended learning design model that can be used effectively and systematically by lecturers in developing blended learning systems for accounting subjects at Institut Teknologi dan Bisnis Ahmad Dahlan Jakarta (ITB-AD). It is important because the research results model can be immediately utilized by accounting lecturers to design blended learning. This study is a design based research in the form of formative research. Data collected by qualitative means through interviews, document analysis, surveys, and observations. The contribution of this study is in the form of a model that is built based on existing learning design models, specifically adapted for learning accounting subjects in ITB-AD, according to the characteristics of students, lecturers, and institutions. The model developed is called the BLADe model.
Model Bahan Ajar Bahasa Inggris Untuk SD Berbasis Pendekatan Kontekstual
Authors: Herlina Usman  , Nidya Chandra Muji Utami  , Otto Fajarianto
Published: October 2020

To develop a model of English language teaching materials based on a contextual approach. English learning in elementary school is the mastery and increase in the number and enrichment of vocabulary to support the mastery of the four English language skills, namely speaking, listening, reading and writing. This study uses mixed methods, research and development of teaching materials in research, adapted from the models of Borg and Gall and models of Dick, Carey and Carey. The four stages in this research are (1) the stage of research and information gathering, (2) the stage of draft development, (3) the stage of testing and product revision, (4) the stage of finalization and dissemination. The results of the research and development were obtained in the fifth grade English language teaching material model. Through a series of stages of developing instructional materials, a product of English language teaching materials for the fifth-grade elementary school was obtained based on a contextual approach that fulfills the characteristics of good teaching materials that can be used by fifth grade students throughout Indonesia.
Pengembangan Media Flash Berbasis Komik Dalam Pembelajaran Menyimak Cerita Rakyat
Authors: Fujiastuti Ariesty , Wulandari Yosi , Suwartini Iis
Published: October 2020

Dalam pembelajaran di sekolah, menyimak masih belum direalisasikan dengan baik. Pelajaran menyimak kurang mendapat perhatian dan sering diremehkan oleh siswa dan guru terutama pada sekolah yang belum maju seperti di wilayah pedesaan. Oleh karena itu, dibutuhkan media pembelajaran yang dapat menarik perhatian siswa seperti media pembelajaran dengan menggunakan flash berbasis komik. Tujuan penelitian ini yaitu untuk mengembangkan dan mengetahui kelayakan media flash berbasis komik dalam pembelajaran Cerita Rakyat. Penelitian ini merupakan penelitian R&D dengan menggunakan model 4D. Uji coba produk yaitu ahli media dan materi, dan uji coba pengguna yaitu siswa. Teknik pengumpulan data menggunakan angket Instrumen penelitian yang digunakan yaitu angket. Validasi instrumen menggunakan construct validity. Reliabilitas instrumen menggunakan reliabilitas internal. Teknik analisis data yaitu analisis deskriptif. Tahap akhir dari penelitian dan pengembangan ini adalah uji kelayakan dan efektifitas. Berdasarkan hasil penelitian, dapat diketahui bahwa penilaian tingkat kelayakan media pembelajaran secara keseluruhan diperoleh hasil yang valid dan sangat layak. Kelayakan media pembelajaran ditinjau dari aspek media dan materi termasuk dalam kategori sangat layak sebesar 100,00 %.  Penilaian peserta didik didapatkan penilaian yang sangat layak dengan persentase skor setiap aspek antara lain a) aspek materi termasuk dalam kategori sangat layak sebesar 39 atau 97,5% dan kategori layak sebesar 1 atau 2,5% b) aspek media termasuk dalam kategori sangat layak sebesar 27 atau 67,5% dan kategori layak sebesar 13 atau 32,5% serta c) aspek penilaian media pembelajaran secara  keseluruhan sebesar 37 atau 92,5%, dan kategori layak sebesar 3 atau 7,5%. Oleh karena itu, media ini dapat digunakan sebagai media pembelajaran yang digunakan oleh guru sebagai pegangan dalam mengajar maupun bagi siswa dalam belajar.
Pengaruh Model Pembelajaran dan Kemampuan Berpikir Kreatif Terhadap Hasil Belajar IPA Siswa SMP
Authors: Lagun Siang Jhoni , Sukardjo Moch. , Salenussa Beatrix J.M. , Sudrajat Yayan , Khasanah Uswatun
Published: October 2020

This study aims to determine the influence of learning models (Contextual Teaching and Learning and Quantum Learning) and the ability to think creatively about the learning outcomes of science education in junior high school students in SMPN 226 Jakarta. This research uses treatment design by level with sample number of 48 students at State Junior Secondary School (SMPN) 226 Jakarta. The data analysis was done by Analysis of Variance (ANAVA) two lane. The results showed that (1) Contextual Teaching and Learning model (CTL) was more effective in improving learning outcomes of IPA SMP Inclusion than Quantum Learning model (QL); (2) there was interaction effect between learning model and student's creative thinking ability toward science learning outcomes 3) for junior high school students in inclusion schools with high creative thinking skills more appropriately using Contextual Teaching and Learning models (CTL), (4) and for students with low creative thinking skills more appropriately using Quantum Learning models (QL).
Strategi Pembelajaran Kooperatif Pada Mata Kuliah Penataan Rambut
Authors: Arum Aniesa Puspa
Published: October 2020

This study aims to design and develop cooperative learning strategies in hairstyling subjects in order to increase motivation and learning involvement of students through the implementation of cooperative learning strategies. This type of research is research and development (R&D) using the 4D development model that aims to find, design, develop and disseminate the products produced so that they can be used in the learning process to improve student learning outcomes. The result of this research is design cooperative learning strategy in hairstyling subject, based on the characteristics and components of structured learning strategy.
Frequency of thrombophilia associated genes. Population – based study.
Authors: Wawrusiewicz-Kurylonek Natalia , Kretowski Adam Jacek , Posmyk Renata
Published: October 2020

Background: Thrombophilia is a hypercoagulable state that may have a genetic basis (inherited) or can be acquired. It is a multifactorial condition and only the mutual interactions between the environment and genes may lead to the development of clinical manifestation. This state is the main factor promoting venous (rarely arterial) thromboembolism (VTE). Inherited thrombophilia is mainly associated with two pathogenic variants in the V coagulation factor (FV) and the prothrombin (FII) genes. The aim of our study was to evaluate the frequency of two pathogenic variants in FII and FV genes as inherited thrombophilia factors in a group within the Polish population in comparison with other described populations. Methods: All studied groups consisted of 633 unrelated patients aged between 18 and 70. Individuals in the research group come from the Podlasie region of Poland. Genotyping of FII and FV variants was performed using the 7900HT Fast Real-Time PCR System and were genotyped by TaqMan assay. Results: The pathogenic allele frequency for A allele was 0.03 (3%) and 0.07 (7%) for FII and FV genes, respectively. The GA/AA genotypes (c.*97G>A variant) were observed in only 33 (5.03%) individuals in the studied group. Additionally, the frequency of GA/AA genotypes was over 17.4% in the coagulation factor V. Co-incidence of heterozygous genotype GA of variants FII and FV genes was observed in only 4 subjects. Conclusion: The FII gene variant shown in our study is less frequent than in other European countries (about 6%). In contrast, the A allele of the FV gene occurs with a frequency similar to that of Northern, Central and South Central Europe (about 5%).
High-Throughput Sequencing Identification of Differentially Expressed microRNAs in Metastatic Ovarian Cancer with Experimental Validations
Authors: Gu Yang , Zhang Shulan
Published: October 2020

Background: Ovarian cancer (OC) is a common gynecological cancer and characterized by high metastatic potential. MicroRNAs (miRNAs, miRs) have the promise to be harnessed as prognostic and therapeutic biomarkers for OC. Herein, we sought to identify differentially expressed miRNAs and mRNAs in metastatic OC, and to validate them with functional experiments.Methods: Differentially expressed miRNAs and miRNAs were screened from six pairs of primary OC tissues and metastatic tissues using an miRStar™ Human Cancer Focus miRNA & Target mRNA PCR Array. Then, gene expression profiling results were verified by reverse transcription quantitative polymerase chain reaction (RT-qPCR) and western blot assays. The binding affinity between miR-7-5p and TGFβ2 was validated by dual-luciferase reporter assay. Expression of miR-7-5p and TGFβ2 was manipulated to assess their roles in malignant phenotypes of highly metastatic HO-8910PM cells.Results: MiRNA profiling and sequencing identified 12 miRNAs and 10 mRNAs that were differentially expressed in metastatic tissues. Gene ontology and Pathway analyses determined that 3 differentially expressed mRNAs (ITGB3, TGFβ2 and TNC) were related to OC metastasis. The results of RT-qPCR confirmed that the decrease of miR-7-5p was most significant in OC metastasis, while TGFβ2 was up-regulated in OC metastasis. Moreover, miR-7-5p targeted and negatively regulated TGFβ2. MiR-7-5p overexpression accelerated HO-8910PM cell viability and invasion, and TGFβ2 overexpression reversed the results. Meanwhile, simultaneous miR-7-5p and TGFβ2 overexpression rescued the cell activities.Conclusions: This study characterizes differentially expressed miRNAs and mRNAs in metastatic OC, where miR-7-5p and its downstream target were most closely associated with metastatic OC. Overexpression of miR-7-5p targets and inhibits TGFβ2 expression, thereby inhibiting the growth and metastasis of OC.
Differences and similarities of high-resolution computed tomography features between Pneumocystis pneumonia and Cytomegalovirus pneumonia in AIDS patients
Authors: Du Chunjing , Liu Jingyuan , Chen Hui , Yan Shuo , Pu Lin , Xiong Haofeng , Xiang Pan , Li Chuansheng , Zhang Ming , Xie Ruming , Chen Budong , Li Ang
Published: October 2020

Background: Accurately differentiating pneumocystis from cytomegalovirus pneumonia is crucial for correct therapy selection in AIDS patients. Hence, the goal of this study was to compare the computerized tomography (CT) features of pneumocystis pneumonia and cytomegalovirus pneumonia in AIDS patients and identify clinical hallmarks to accurately distinguish these two pathologies.Methods: A total of 112 AIDS patients (78 with pneumocystis pneumonia and 34 cytomegalovirus pneumonia) at Beijing Ditan Hospital from January 2017 to May 2019 were included in this study. Two experienced chest radiologists retrospectively reviewed CT images for 17 features including ground-glass opacity, consolidation, nodules, and halo sign. Significance was calculated by the chi-square (χ2) test. Binary logistic regression analyses were conducted to identify the significant parameters that distinguished pneumocystis pneumonia from cytomegalovirus pneumonia. Correlations were analyzed by Pearson or Spearman correlation analyses. Result were considered significant if P < 0.05.Results: The presence of consolidation, halo signs, and nodules (all P＜0.05) were significantly more frequent in patients with cytomegalovirus pneumonia than in those with pneumocystis pneumonia. Small nodules (32.5% in cytomegalovirus pneumonia, 6.41% in pneumocystis pneumonia, P＜0.001) without perilymphatic distribution were particularly common in patients with cytomegalovirus pneumonia. Large nodules were not found in any of patients with cytomegalovirus pneumonia. The presence of ground-glass opacity, reticulation, and bronchial wall thickening (all P > 0.05) were common in both groups. Conclusions: Analysis of consolidation, nodules, and halo signs may contribute to the differential diagnosis of pneumocystis pneumonia or cytomegalovirus pneumonia. However, some CT features considered typical in one or other diseases appear with similar frequency in both cohorts of AIDS patients. CT features are potentially useful for the differential diagnosis of pneumocystis pneumonia and cytomegalovirus pneumonia in AIDS patients. Add potential implications
miR-105-3p acts an oncogene to promote proliferation and metastasis of breast cancer cell by targeting GOLIM4
Authors: Lin Bo , Shi Enyi , Jin Qiu , Zhao Wenhui , Wang Juan , Ji Runyuan
Published: October 2020

Background:Dysregulation of miRNAs is involved in carcinogenesis of breast and may be used as prognostic biomarkers and therapeutic targets during cancer process. The purpose of this study was to explore the effect of miR-105-3p on tumourigenicity of breast cancer and its underlying molecular mechanisms.Methods:Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was applied to detect the expression of miR-105-3p in breast cancer tissues and cell lines. The impacts of miR-105-3p on proliferation, migration, invasion and apoptosis of human breast cancer cells (MCF-7 and ZR-75-30) were evaluated by CCK-8, transwell chamber assay, TUNEL assay and western blot assay, respectively. Besides, bioinformatics and luciferase reporter assay were used to find out the target genes of miR-105-3p.Results:The expression of miR-105-3p was elevated in breast cancer tissues and increased along with tumor severity. Downregulation of miR-105-3p could inhibit cell proliferation, suppress cell migration/invasion, and promote cell apoptosis in MCF-7 and ZR-75-30 cells. Furthermore, Golgi integral membrane protein 4 (GOLIM4) was identified to be the direct target gene of miR-105-3p by bioinformatics and luciferase reporter assay. In addition, silencing of GOLIM4 could restore the anti-breast cancer effects induced by miR-105-3p downregulation.Conclusions:miR-105-3p acts an oncogene to promote proliferation and metastasis of breast cancer cell by targeting GOLIM4, which provides a new target for the prevention and treatment of breast cancer.
NFAT2 overexpression suppresses the malignancy of hepatocellular carcinoma through inducing Egr2 expression
Authors: Wang Jian , Zhang Yamin , Liu Lei , Cui Zilin , Shi Rui , Hou Jianchun , Liu Zirong , Yang Long , Wang Lianjiang , Li Yang
Published: October 2020

BACKGROUND: Nuclear factor of activated T cells 2 (NFAT2) has been reported to regulate the development and malignancy of few tumors. In this study, we aimed to explore the effect of NFAT2 expression on cell fate of HepG2 cell and its potential mechanisms.METHODS: Firstly, the pcDNA3.1-NFAT2 plasmid was transfected into HepG2 cells to construct NFAT2 overexpressed HepG2 cells. Then, the chemical count kit-8 cell viability assay, Annexin V-FITC apoptosis detection, EdU labeling proliferation detection, transwell and wound healing experiments were performed. The expression of Egr2 and FasL, and the phosphorylation of AKT and ERK, after ionomycin and PMA co-stimulation, was detected, while the Ca2+ mobilization stimulated by K+ solution was determined. At last, the mRNA and protein expression of NFAT2, Egr2, FasL, COX-2 and c-myc in carcinoma and adjacent tissues was investigated.RESULTS: The NFAT2 overexpression suppressed the cell viability, invasion and migration capabilities, and promoted apoptosis of HepG2 cells. NFAT2 overexpression induced the expression of Egr2 and FasL and suppressed the phosphorylation of AKT and ERK. The sensitivity and Ca2+ mobilization of HepG2 cells was also inhibited by NFAT2 overexpression. Compared with adjacent tissues, the carcinoma tissues expressed less NFAT2, Egr2, FasL and more COX-2 and c-myc.CONCLUSION: The current study firstly suggested that NFAT2 suppressed the aggression and malignancy of HepG2 cells through inducing the expression of Egr2. The absence of NFAT2 and Egr2 in carcinoma tissues reminded us that NFAT2 may be a promising therapeutic target for hepatocellular carcinoma treatment.
Differential gene expression in skin RNA of horses affected with degenerative suspensory ligament desmitis
Authors: Haythorn Abigail , Young Madeline , Stanton James , Zhang Jian , Mueller P.O.E. , Halper Jaroslava
Published: October 2020

Background: Equine Degenerative Suspensory Desmitis (DSLD) is a systemic connective tissue disorder first identified in Peruvian Paso horses, but afflicting other horse breeds as well. Inappropriate accumulation of proteoglycans in connective tissues, most prominently in tendons and ligaments leads to progressive and debilitating lameness and pain. It is largely unknown what drives the overproduction of proteoglycans, but our previous studies suggest involvement of bone morphogenetic protein 2 (BMP2), a member of the transforming growth factor β (TGFβ) family, impacting synthesis of proteoglycans. To identify potential players in pathogenesis of DSLD a new approach utilizing next generation sequencing was undertaken. Methods: Next generation sequencing was performed using RNA extracted from skin biopsies of six control Peruvian Pasos and six horses with DSLD (4 Peruvian Pasos and 2 Warmbloods). The CuffDiff result sets were validated with algorithms used to run them. This was based on the determined False Discovery Rates derived from the P-values adjusted for multiple testing for any given result.Results: Bioinformatics analysis of transcriptomes revealed differential expression of over 1500 genes, including increased expression of genes for several growth factors (most prominently BMP2, FGF5, CTGF, many members of the EGF family), and mediators of signaling (Fos, Myc, MAPK system), and keratins. Two genes encoding for enzymes involved in synthesis of hyaluronan were also overexpressed. Gene expression was decreased for protein cores of many proteoglycans, several growth factors, most collagens and many peptides with immune function. Conclusions: The overexpression of BMP2 correlates well with our previous data. However, the decrease in expression of numerous proteoglycans was unexpected. A mutation in a gene of a less characterized proteoglycan and/or glycosyltransferase with subsequent increased production of hyaluronan and/or a proteoglycan(s) undetected in our study could account for the systemic proteoglycan deposition. Decreased collagen gene expression indicates abnormal connective tissue metabolism. The increased expression of keratin genes and FGF5 supports reports of skin abnormalities in DSLD. Underexpression of immune function genes corresponds with lack of inflammation in DSLD tissues. Finally, though the proteoglycan and/or glycosaminoglycan abundant in DSLD has not been identified, we validated our previous data, including overexpression of BMP2, and systemic nature of DSLD due to disturbed metabolism of the extracellular matrix.
BTG1 inhibits malignancy as a novel prognosis signature in endometrial carcinoma
Authors: Li Yibing , Huo Jianing , He Junjian , Zhang Yunzheng , Ma Xiaoxin
Published: October 2020

Background: Endometrial carcinoma (EC) is one of the three major malignant tumors of the female reproductive system. In recent years, the incidence and mortality rate of EC have increased. B-cell translocation gene 1 (BTG1) is an anti-proliferation gene that regulates the occurrence and development of a variety of tumors, but there is no research regarding this gene in EC.Methods: Based on The Cancer Genome Atlas (TCGA) database, we used a variety of bioinformatics tools and databases to explore the expression and prognosis of BTG1. We verified expression and prognosis of BTG1 in EC using qRT-PCR and analyzed the relevant clinicopathological parameters. We functionally enriched BTG1 and related genes in EC patients through the bioinformatics website and analyzed miRNA targets of BTG1 and interacting protein networks. Cell proliferation, wound healing, transwell invasion, and cell apoptosis assays were used to detect the effects of BTG1 on the malignant biological behavior of endometrial carcinoma cells (ECCs). The effect of BTG1 on the epithelial-to-mesenchymal transition (EMT) process was detected using western blot.Results: We analyzed the expression and prognosis of BTG1 based on TCGA and found that low expression of BTG1 was associated with poor EC prognosis. The qRT-PCR suggested that BTG1 had low expression in EC. BTG1 expression was significantly correlated with overall survival (OS) shortening. Clinicopathological analysis suggested that expression of BTG1 was related to invasion depth and the International Federation of Gynecology and Obstetrics (FIGO) stage. EC pathological tissue type, fertility history, lymphatic metastasis, menopause, estrogen receptor (ER), progesterone receptor (PR), and age of diagnosis were not related. Functional enrichment analysis showed that BTG1 plays an important role in regulating embryonic development, tumorigenesis, apoptosis, and cell cycle. Biological behavior experiments suggest that BTG1 inhibits proliferation, migration, and invasion of ECCs, and promotes apoptosis of ECCs. Western blot indicated that BTG1 inhibited the EMT process of ECCs.Conclusions: BTG1, as a tumor suppressor gene, plays an important role in the occurrence and development of EC. We believe that BTG1 can be used as a potential prognostic biomarker for EC.
Distally based peroneal artery perforator-plus fasciocutaneous flap in the reconstruction of soft tissue defects over the distal forefoot: a retrospectively analyzed clinical trial
Authors: Peng Ping , Luo Zhaobiao , Lv Guohua , Ni Jiangdong , Wei Jianwei , Dong Zhonggen
Published: October 2020

Background Distally based peroneal artery perforator-plus fasciocutaneous (DPAPF) flaps are widely used for reconstructing soft-tissue defects of the lower extremity. However, reports on the reconstruction of the defects over the distal forefoot using the DPAPF flaps are scarce. Herein, we describe our experience on the reconstruction of these defects using DPAPF flaps in a considerable sample size. Methods Between February 2005 and August 2019, a total of 56 DPAPF flaps in 56 patients were used to reconstruct soft-tissue defects in the forefoot. In order to reduce the length of fascial pedicle and the total length of the DPAPF flaps, the ankles were fixed in dorsiflexion using a Kirschner wire before designing the flaps. The flaps were elevated by the anterograde–retrograde approach. Patient factors and flap factors were compared between the “survival” and “partial necrosis” groups. Results Overall, 47 flaps had survived completely in one stage. Partial necrosis developed in nine flaps, with only one remnant defect covered using a local flap. By fixing the ankles in dorsiflexion, the length of the fascial pedicle was reduced approximately 2.35 ± 0.58 cm, the total length of the flap was simultaneously shortened by the same amount as the length of the fascial pedicle. The width of the fascia pedicle varied from 3.0 cm to 6.0 cm. The fascial pedicle width > 4 cm was found in 21 flaps. The partial necrosis rate of the DPAPF flaps with the top-edge located in the 8th zone was significantly lower than that in the 9th zone (p < 0.05).Conclusions The DPAPF flaps can be effectively used to reconstruct the defects over the distal forefoot because of convenient harvest and reliability. By fixing ankle in dorsiflexion with Kirschner wire and widening the fascial pedicle appropriately, the top-edge and LWR of the flaps will be decreased, and thus the procedures are helpful for the flaps survival.