bluuebunny/crossref_metadata_2025 huggingface dataset:
Action at a distance: Long-distance rate adaptation in event perception
Authors: Heffner Christopher C , Newman Rochelle S , Idsardi William J
Published: September 2020

Viewers’ perception of actions is coloured by the context in which those actions are found. An action that seems uncomfortably sudden in one context might seem expeditious in another. In this study, we examined the influence of one type of context: the rate at which an action is being performed. Based on parallel findings in other modalities, we anticipated that viewers would adapt to the rate at which actions were displayed at. Viewers watched a series of actions performed on a touchscreen that could end in actions that were ambiguous to their number (e.g., two separate “tap” actions versus a single “double tap” action) or identity (e.g., a “swipe” action versus a slower “drag”). In Experiment 1, the rate of actions themselves was manipulated; participants used the rate of the actions to distinguish between two similar, related actions. In Experiment 2, the rate of the actions that preceded the ambiguous one was sped up or slowed down. In line with our hypotheses, viewers perceived the identity of those final actions with reference to the rate of the preceding actions. This was true even in Experiment 3, when the action immediately before the ambiguous one was left unmodified. Ambiguous actions embedded in a fast context were seen as relatively long, while ambiguous actions embedded in a slow context were seen as relatively short. This shows that viewers adapt to the rate of actions when perceiving visual events.
Home Modification Assessments for Accessibility and Aesthetics: A Rapid Review
Authors: Struckmeyer Linda , Morgan-Daniel Jane , Ahrentzen Sherry , Ellison Carlyn
Published: September 2020

Purpose:

The purpose of this study was to identify assessments used to evaluate the homes of people with disabilities in terms of accessibility, usability, activities, comfort/satisfaction, and aesthetics.

Background:

The home is increasingly becoming an environment for healthcare as more people desire to age in place. Research indicates home environmental modifications to be beneficial to promote a better person–environment fit, especially when using a standardized assessment approach. There is not a comprehensive list of assessments that address home modifications, adaptations, or interior designs for people with disabilities.

Method:

Researchers conducted a rapid review of articles, with data collection scales, instruments, and procedures for home modifications published between 2000 and 2017.

Results:

A total of 26 articles met the inclusion criteria, resulting in the identification of 33 distinct assessments, including 18 assessments evaluating the accessibility of home modifications, 3 assessments examining usability, 15 assessments addressing activities of daily living or functional activities, and 5 assessments addressing comfort and/or satisfaction. No assessments for aesthetics were located.

Conclusion:

Researchers developed a list of assessments that could be used for research or practice. Further research is needed to address the lack of assessments focusing on the aesthetics or attractiveness of home modifications, as well as more assessments tailored to specific diagnoses and population groups.
Noise in Maternity Wards: A Research on Its Contributors and Sources
Authors: Ban Qichao , Chen Bing , Kang Jian , Zhang Yiping , Li Jie , Yao Jiawei
Published: September 2020

Aims:

This research aimed to investigate the major user behavior patterns of noise sources in healthcare environments and summarize such information as evidence that can inform the design of maternity wards for indoor noise control and patients’ well-being.

Methods:

Field investigations were conducted to identify users’ behaviors as the major contributors of noises in the maternity wards of a typical hospital. A control experiment was set to test the feasibility of a noise control system that consisted of smart bracelets, mobile terminals, and monitors. Comparative studies were designed for statistical analysis of patients’ sleep quality and satisfaction. Finally, a follow-up interview was conducted among the experts who were from the fields of healthcare environment design, medical treatments, and hospital administration to shed an insight into their concerns on the findings.

Results:

The enclosed waiting areas, instead of open ones that were often seen in hospitals, around the entrances of operation rooms, were considered as the appropriate design strategy for maternity wards in China. Such a design could keep patients from being exposed to the excessive noises generated by visitors during nighttime, although it would occupy the floor area of wards and lead to a reduction of beds. Moreover, the statistical information of patients’ behaviors could be used to moderate visitors’ behaviors.

Conclusions:

It was necessary to include user behavior information in building information management and then make a good trade-off between the proportions of wards and enclosed waiting rooms in order to achieve a balance of medical efficiency and environmental satisfaction.
Scholar-activist perspectives on radical food geography: collaborating through food justice and food sovereignty praxis
Authors: Levkoe Charles Z. , Hammelman Colleen , Reynolds Kristin , Brown Xavier , Chappell M. Jahi , Salvador Ricardo , Wheeler Beverly
Published: September 2020

Radical geography research, teaching, and action have increasingly focused on food systems, examining the scalar, sociopolitical, and ecological dynamics of food production and harvesting, processing, distribution, consumption, and waste. While academics have contributed significantly to these debates, the success and progress of this scholarship cannot be separated from the work of practitioners and activists involved in food justice and food sovereignty movements. This paper draws together the voices of scholars and activists to explore how collaborations can productively build the evolving field of radical food geography and contribute to more equitable and sustainable food systems for all. These perspectives provide important insight but also push the boundaries of what is typically considered scholarship and the potential for impacts at the levels of theory and practice. Reflecting on the intersecting fields of radical geography and food studies scholarship and the contributions from the scholar-activists, the authors share a collective analysis through a discussion of the following three emerging themes of radical food geography: (1) a focus on historical and structural forces along with flows of power; (2) the importance of space and place in work on food justice and food sovereignty; and (3) a call to action for scholars to engage more deeply with radical food systems change within their research and teaching process but also in response to it.
To die a thousand deaths: COVID-19, racial capitalism, and anti-Black violence
Authors: Liebman Alexander , Rhiney Kevon , Wallace Rob
Published: September 2020

In this short commentary, we discuss the ways in which racial capitalism has developed a historical and ecological landscape through which COVID-19 has emerged, spread, and created uneven impacts across long-standing racial divisions inseparable from capitalist accumulation and expansion. We argue that this perspective on COVID-19 (and infectious disease in general) creates strong alliances with calls for reparations rooted in abolition of policing and incarceration, especially in light of the conjuncture of pandemic and uprising in response to police violence in the United States context. By bringing long-standing Marxian notions of alienation and metabolic rift, crucial for understanding the ecological and epistemic fissures leading to COVID-19’s spread, in conversation with writing on racial capitalism and anti-Blackness, we hope to illuminate pathways to a more grounded historical and ecological analysis that supports a radical vision of ecosystem health.
The Implementation of COVID-19 Social Distancing Measures Changed the Frequency and the Characteristics of Facial Injury: The Newcastle (Australia) Experience
Authors: Wang Che-Jen , Hoffman Gary R. , Walton Gary M.
Published: September 2020

Study Design:

A retrospective study was undertaken of a cohort of facially injured patients using matched 8-week periods: non-COVID (2019) and COVID (2020).

Objective:

To determine whether there were any changes to the frequency and characteristics of facial injury due to the imposition of COVID-19 social distancing measures.

Methods:

The primary predictor variable was an 8-week period of COVID-19 social distancing. The primary outcome variable was the sustaining of a facial injury. Demographic (age/gender) and injury characteristics (mechanism, site, and treatment) were also studied. Descriptive statistical analysis was undertaken and comparison made using Pearson χ2 and Fisher’s exact tests.

Results:

The number of facial injuries decreased from 103 (2019) to 73 (2020). There were statistically significant differences in changes over time for the 8-week periods. There were some clinically apparent differences seen in the characteristics of facial injuries.

Conclusions:

The imposition of COVID-19 social distancing changed the frequency and characteristics of facial injury.
Peripartum clinical manifestations of a mesentericorenocaval shunt in a Burmese cat
Authors: Hoon QiCai J , Siow Jia Wen , Jenkins Elizabeth , So Wilson , Krockenberger Mark , Makara Mariano , Brunel Laurencie
Published: September 2020

Case summary

A 3-year-old entire female Burmese cat was presented for investigation of intermittent lethargy during gestation followed by persistent hypersalivation and ataxia postpartum. The cat had queened three litters in total, with clinical signs worsening during the most recent lactation period. Mild anaemia (26%), hypoglycaemia (2.4 mmol/l; reference interval [RI] 3.9–8.3 mmol/l) and increased postprandial serum bile acids (74 µmol/l; RI <25 µmol/l) were identified on initial bloodwork. Multiphase contrast-enhanced CT identified a mesentericorenocaval portosystemic shunt; this was attenuated surgically with an ameroid constrictor. Clinical signs resolved after surgery. Follow-up 3 months postoperatively revealed normal pre- and postprandial serum bile acids (2 µmol/l and 3 µmol/l, respectively) with repeat CT identifying evidence of shunt attenuation. The cat continued to be healthy and free of clinical signs 12 months postoperatively.

Relevance and novel information

Mesentericorenocaval portosystemic shunt morphology has not been previously reported in the cat and should be considered as a differential diagnosis for cats presenting with peripartum onset of malaise, ptyalism or ataxia.
The Outsourcing of Online Dating: Investigating the Lived Experiences of Online Dating Assistants Working in the Contemporary Gig Economy
Authors: Rochadiat Annisa M. P. , Tong Stephanie Tom , Hancock Jeffrey T. , Stuart-Ulin Chloe Rose
Published: September 2020

A small cottage industry emerging within the larger gig economy is online dating assistant (ODA) companies that allow paying clients to outsource the labor associated with online dating, including profile development, date selection and matching, and even interaction (i.e., ODAs assume their clients’ identities to exchange messages with other [unsuspecting] daters to secure face-to-face dates). The newness of this industry presents an opportunity to investigate the lived experience of remote employees working in an up-and-coming virtual organization. Through interviews with six ODAs, we explored motivations, day-to-day workflow, and development of work identities. Analysis uncovered unique challenges ODAs faced when performing the “human-based” tasks of online dating, which differed starkly from other popular services being bought and sold in the gig economy (e.g., rideshare, food delivery). Findings also show how ODAs engage in pragmatic and critical sensemaking as they navigate the specific challenges associated with ODA labor, and those created by remote work and gig labor, more generally.
Thoracic Reciprocal Change Can Be Predicted Before Surgery in Adult Spinal Deformity
Authors: Ishikawa Koji , Nakao Yusuke , Oguchi Fumihiko , Toyone Tomoaki , Sano Shigeo
Published: September 2020

Study Design:

Retrospective cohort study.

Objective:

Analysis of postoperative sagittal alignment of the unfused spine is lacking in patients with adult spinal deformity (ASD). The present study aims to evaluate the efficacy of the whole spine full-flexion lateral radiograph to predict the reciprocal change of the unfused spine after correction surgery. We hypothesized that the novel parameter (T1-UIV angle: angle between the upper vertebral endplate of the T1 and the upper vertebral endplate of the upper instrumented vertebra) of the preoperative whole spine full-flexion lateral radiograph is similar to that of the postoperative lateral radiograph if the patient has the ideal sagittal alignment.

Methods:

Twenty-six ASD patients who underwent correction surgery with a minimum 2-year follow-up were enrolled and separated into the Ideal and Non-Ideal groups according to the Scoliosis Research Society (SRS)-Schwab classification of the final follow-up radiograph. Radiographic parameters, including T1-UIV of the preoperative whole spine full-flexion lateral radiograph, were obtained.

Results:

Thirteen patients were included in the Ideal group and 13 were in Non-Ideal group. Preoperative T1-UIV of the whole spine full-flexion lateral radiograph exhibited significant correlations with the T1-UIV angles of the postoperative and final follow-up radiographs ( r = 0.64, P < .01, y = 0.800 x + 8.012, and r = 0.69, P < .01, y = 0.857 x + 2.960, respectively). Interestingly, this correlation was stronger for the Ideal group ( r = 0.77, P < .01, y = 1.207 x − 1.517, and r = 0.89, P < .01, y = 0.986 x + 0.694, respectively).

Conclusion:

A novel radiographic strategy (T1-UIV of preoperative the whole spine full-flexion lateral radiograph) could estimate the postoperative alignment of the unfused spine correctly.
The Role of Spinal Orthoses in Osteoporotic Vertebral Fractures of the Elderly Population (Age 60 Years or Older): Systematic Review
Authors: Kweh Barry Ting Sheen , Lee Hui Qing , Tan Terence , Rutges Joost , Marion Travis , Tew Kim Siong , Bhalla Vikram , Menon Shyaman , Oner Fetullah Cumhur , Fisher Charles , Tee Jin Wee
Published: September 2020

Study Design:

Systematic review.

Objective:

Spinal orthoses have been generally used in the management of osteoporotic vertebral fractures in the elderly population with purported positive biomechanical and functional effects. To our knowledge, this is the first systematic review of the literature examining the role of spinal orthoses in osteoporotic elderly patients who sustain low energy trauma vertebral fractures.

Methods:

A systematic literature review adherent to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines was conducted. Methodical searches utilizing MEDLINE, EMBASE, Google Scholar, and Cochrane Databases was performed.

Results:

Of the 2019 articles initially retrieved, 7 published articles (4 randomized controlled trials and 3 prospective cohort studies) satisfied the inclusion criteria. Five studies reported improvement in quantitative measurements of spinal column stability when either a rigid or semirigid orthosis was used, while 1 study was equivocal. The studies also showed the translation of biomechanical benefit into significant functional improvement as manifested by improved postural stability and reduced body sway. Subjective improvement in pain scores and quality of life was also noted with bracing.

Conclusion:

The use of spinal orthoses in neurologically intact elderly patients aged 60 years and older with osteoporotic compression vertebral fractures results in improved biomechanical vertebral stability, reduced kyphotic deformity, enhanced postural stability, greater muscular strength and superior functional outcomes.
Treatment of adjacent segment disease with percutaneous transforaminal endoscopic discectomy: Early experience and results
Authors: Kapetanakis Stylianos , Gkantsinikoudis Nikolaos , Gkasdaris Grigorios , Charitoudis Georgios
Published: September 2020

Purpose:

Adjacent segment disease (ASD) constitutes a long-term complication of instrumented spinal fusion. Aim of this study is to analyze the utilization of percutaneous transforaminal endoscopic discectomy (PTED) for the treatment of symptomatic ASD, emphasizing basically in the postoperative course.

Methods:

A prospective study with 35 patients was designed. Patients enrolled in our study were distributed in two different groups. Group A constituted of 15 patients featuring ASD as a complication of a previously conducted lumbar spinal fusion. Group B was composed of 20 patients, presenting simple lumbar disc herniation (LDH). All patients were subjected to successful PTED for LDH. Patients were evaluated at regular intervals in 6-week and 3-, 6-, and 12-month postoperatively. Visual analog scale was utilized for leg (VAS-LP) and low back pain (VAS-BP) evaluation. Health-related quality of life was assessed with short-form 36 health survey questionnaire (SF-36).

Results:

VAS-BP scores were statistically significantly differentiated between the two groups in all intervals of follow-up. In contrast, VAS-LP scores demonstrated statistically significant differentiation in none of follow-up intervals, indicating similar results between the two groups. Maximal improvement was in both cases for all patients observed in 6-week postoperatively, with subsequent stabilization. SF-36 preoperative evaluation denoted a statistically significant differentiation in bodily pain and role emotional parameters, which was continually until the end of follow-up observed. The other SF-36 parameters featured similar values between the two groups preoperatively as well as during the follow-up intervals.

Conclusion:

PTED is capable of successfully dealing with LDH and furthermore with complications of fusion as ASD.
From Sequence Data to Patient Result: A Solution for HIV Drug Resistance Genotyping With Exatype, End to End Software for Pol-HIV-1 Sanger Based Sequence Analysis and Patient HIV Drug Resistance Result Generation
Authors: Kingwara Leonard , Karanja Muthoni , Ngugi Catherine , Kangogo Geoffrey , Bera Kipkerich , Kimani Maureen , Bowen Nancy , Abuya Dorcus , Oramisi Violet , Mukui Irene
Published: September 2020

Introduction:

With the rapid scale-up of antiretroviral therapy (ART) to treat HIV infection, there are ongoing concerns regarding probable emergence and transmission of HIV drug resistance (HIVDR) mutations. This scale-up has to lead to an increased need for routine HIVDR testing to inform the clinical decision on a regimen switch. Although the majority of wet laboratory processes are standardized, slow, labor-intensive data transfer and subjective manual sequence interpretation steps are still required to finalize and release patient results. We thus set out to validate the applicability of a software package to generate HIVDR patient results from raw sequence data independently.

Methods:

We assessed the performance characteristics of Hyrax Bioscience’s Exatype (a sequence data to patient result, fully automated sequence analysis software, which consolidates RECall, MEGA X and the Stanford HIV database) against the standard method (RECall and Stanford database). Exatype is a web-based HIV Drug resistance bioinformatic pipeline available at sanger. exatype.com . To validate the exatype, we used a test set of 135 remnant HIV viral load samples at the National HIV Reference Laboratory (NHRL).

Result:

We analyzed, and successfully generated results of 126 sequences out of 135 specimens by both Standard and Exatype software. Result production using Exatype required minimal hands-on time in comparison to the Standard (6 computation-hours using the standard method versus 1.5 Exatype computation-hours). Concordance between the 2 systems was 99.8% for 311,227 bases compared. 99.7% of the 0.2% discordant bases, were attributed to nucleotide mixtures as a result of the sequence editing in Recall. Both methods identified similar (99.1%) critical antiretroviral resistance-associated mutations resulting in a 99.2% concordance of resistance susceptibility interpretations. The Base-calling comparison between the 2 methods had Cohen’s kappa (0.97 to 0.99), implying an almost perfect agreement with minimal base calling variation. On a predefined dataset, RECall editing displayed the highest probability to score mixtures accurately 1 vs. 0.71 and the lowest chance to inaccurately assign mixtures to pure nucleotides (0.002–0.0008). This advantage is attributable to the manual sequence editing in RECall.

Conclusion:

The reduction in hands-on time needed is a benefit when using the Exatype HIV DR sequence analysis platform and result generation tool. There is a minimal difference in base calling between Exatype and standard methods. Although the discrepancy has minimal impact on drug resistance interpretation, allowance of sequence editing in Exatype as RECall can significantly improve its performance.
Judgments of Economic Fairness Are Based More on Perceived Economic Mobility Than Perceived Inequality
Authors: Heiserman Nicholas , Simpson Brent , Willer Robb
Published: September 2020

Are judgments of the fairness of the American economy based on perceptions of economic inequality, mobility, or both? In two experiments, the authors varied information on levels of U.S. inequality and mobility, measuring effects on individuals’ judgments of economic fairness and meritocracy. Although both treatments influenced perceptions of economic fairness and meritocracy, the mobility effect was generally larger. The two treatments did not interact, countering a common claim that high social mobility legitimizes high inequality. Effects on preferences for government action to reduce inequality and increase mobility were weak or nonexistent. Additional conditions that measured, rather than manipulated, inequality and mobility perceptions showed that respondents generally perceived inequality to be very high but were more optimistic about the level of mobility. Our studies suggest that Americans’ optimism about economic mobility does more to mitigate concerns about economic fairness than does underestimation of economic inequality.
Process Multiplicity and Process Dynamics: Weaving the Space of Possible Paths
Authors: Pentland Brian T. , Mahringer Christian A. , Dittrich Katharina , Feldman Martha S. , Wolf Julie Ryan
Published: September 2020

In research on process organization studies, the concept of multiplicity is widely used, but a fundamental confusion about what process multiplicity means persists. As a result, we miss some of the potential of this concept for understanding process dynamics and process change. In this paper, we define process multiplicity as a duality of ‘one’ and ‘many’, and we conceptualize ‘the many’ as a space of possible paths encompassed by a process. We use the notion of paths to operationalize process multiplicity and make it accessible for empirical research. When we see process as a multiplicity, process change can be understood as expanding, shifting or contracting the space of possible paths. We suggest that this concept of process multiplicity also has implications for a range of other theoretical and practical topics, including standards, standardization and flexibility as well as process replication, management and resilience.
The role of host defences in Covid 19 and treatments thereof
Authors: Dattilo Maurizio
Published: September 2020

Hydrogen sulfide (H2S) is a natural defence against the infections from enveloped RNA viruses and is likely involved also in Covid 19. It was already shown to inhibit growth and pathogenic mechanisms of a variety of enveloped RNA viruses and it was now found that circulating H2S is higher in Covid 19 survivors compared to fatal cases. H2S release is triggered by carbon monoxide (CO) from the catabolism of heme by inducible heme oxygenase (HO-1) and heme proteins possess catalytic activity necessary for the H2S signalling by protein persulfidation. Subjects with a long promoter for theHMOX1gene, coding for HO-1, are predicted for lower efficiency of this mechanism. SARS-cov-2 exerts ability to attack the heme of hemoglobin and other heme-proteins thus hampering both release and signalling of H2S. Lack of H2S-induced persulfidation of the KATPchannels of leucocytes causes adhesion and release of the inflammatory cytokines, lung infiltration and systemic endothelial damage with hyper-coagulability. These events largely explain the sex and age distribution, clinical manifestations and co-morbidities of Covid-19. The understanding of this mechanism may be of guidance in re-evaluating the ongoing therapeutic strategies, with special attention to the interaction with mechanical ventilation, paracetamol and chloroquine use, and in the individuation of genetic traits causing increased susceptibility to the disruption of these physiologic processes and to a critical Covid 19. Finally, an array of therapeutic interventions with the potential to clinically modulate the HO-1/CO/H2S axis is already available or under development. These include CO donors and H2S donors and a boost to the endogenous production of H2S is also possible.
Modifying elastic modulus of two-component polyurethane adhesive for structural hardwood bonding
Authors: Bockel S. , Harling S. , Konnerth J. , Niemz P. , Weiland G. , Hogger E. , Pichelin F.
Published: September 2020

Subject to this study is the modification of an experimental two-component polyurethane (2C PUR) as an alternative adhesive for structural hardwood bonding. The 2C PUR has been adapted by calcium carbonate as filler to increase its modulus of elasticity with the aim of increasing the modulus analogue to the ones typically observed for classic amino- and phenol based adhesives. The 2C PUR system was compared with a commercial one-component polyurethane (1C PUR) and a phenol resorcinol formaldehyde (PRF) adhesive. The wetting properties of the adhesives were tested in terms of surface tension, polar and dispersive part and contact angle on European beech wood (Fagus sylvatica L.). In addition, adhesive polymer films of 2C PUR were tested for tensile strength and modulus of elasticity (E-Modulus) following ISO 527-1. The adhesives bond performance on beech wood was determined by lap-joints according to EN 302-1 in various climate conditions. The results show that 2C PUR has proper wettability properties on beech wood. Adding 60% wt filler to the polyol component increased the E-Modulus from 2.3 GPa (0%) to 4.4 GPa. The tensile strength of the modified 2C PUR polymer films was comparable with the industrial 1C PUR. Tensile shear strength and wood failure percentage of 2C PUR lap-joints were increased by adding filler and met requirements in dry and re-dried conditions according to EN 302-1. However, the addition of filler didn’t result in an improvement in wet conditions. The present study shows sufficient performance for bonding hardwood with 2C PUR in dry conditions, while the system still needs to be improvement regarding its performance in humid conditions.
My Migraine Voice survey: disease impact on healthcare resource utilization, personal and working life in Finland
Authors: Sumelahti Marja-Liisa , Sumanen Markku , Sumanen Merika S. , Tuominen Samuli , Vikkula Johanna , Honkala Sanna M. , Rosqvist Stina , Korolainen Minna A.
Published: September 2020

Background

A global My Migraine Voice survey was conducted in 31 countries among 11,266 adults who suffered from ≥4 monthly migraine days (MMD). The aim of this retrospective observational survey-based study was to analyse the country specific results in Finland in order to understand the impact of migraine based on disease severity.

Methods

The included participants (3%, n = 338/11,266) were stratified by mean MMDs into 4 ≤ MMD < 8 (n = 133), 8 ≤ MMD < 15 (n = 139) and MMD ≥ 15 (n = 66) subgroups. Comorbidities, migraine-related emotional burden and impact on daily living and work productivity and activity impairment (WPAI) were assessed. Subgroup analysis on healthcare resource utilization (HCRU) due to migraine was assessed by visits to healthcare practitioners (HCPs) during the past 6 months and by hospitalizations and emergency room (ER) visits during the past 12 months. The group difference was tested using the one-way ANOVA and for categorical variables using the Chi-squared test. The association between HCRU and MMD and number of comorbidities was assessed using negative binomial regression analysis.

Results

Mean age was 44 years, 93% were women and 67% (n = 227) were employed. Chronic migraine (CM, MMD ≥ 15) was reported in 19.5% of the respondents. The negative impact on daily functioning and emotional burden increased significantly by migraine frequency. Mean number of comorbidities was 2.4, and mean number of HCP visits during the previous 6 months was 5.9. Increase in migraine frequency and comorbidities was associated with higher HCRU. Eighty-eight percent of the respondents reported negative impact on working life and 52% experienced overall work productivity impairment. Over previous month, the mean number of missed working days for all respondents was 2.8 days of which 54% were paid sick leave days, and in CM up to 6.0 days and 30%, respectively. Both absenteeism and presenteeism were higher in the CM group.

Conclusions

The emotional and functional burden was high, and the societal burden increased by frequency and severity of migraine, as shown by higher HCRU and reduced work productivity. There is a need to improve quality of care in migraine and improve migraine management related issues in both healthcare and society in Finland.
Adverse effects in the fish embryo acute toxicity (FET) test: a catalogue of unspecific morphological changes versus more specific effects in zebrafish (Danio rerio) embryos
Authors: von Hellfeld Rebecca , Brotzmann Katharina , Baumann Lisa , Strecker Ruben , Braunbeck Thomas
Published: September 2020

Background

The Fish Embryo Acute Toxicity (FET) test with the zebrafish (Danio rerio) embryo, the OECD test guideline (TG) 236, has been designed as an alternative for acute fish toxicity testing such as the OECD Acute Fish Toxicity Test (TG 203). To provide equivalent sensitivity to the acute fish test, the original FET test was designed to use only four morphological core endpoints: coagulation of the embryo, lack of somite formation, lack of heart beat, and non-detachment of the tail. These endpoints were selected due to (1) their association with mortality, directly or indirectly, (2) improve the practicality for screening by well-trained technical staff, and (3) the endpoints being relatively simple morphological alterations.

Results

With the growing need to understand the developmental toxicity of compounds found in the environment, the FET protocol has repeatedly been extended to a multitude of additional morphological endpoints that also allow the monitoring of teratogenicity. As the extensive use of the FET test has generated a multitude of observations in the scientific literature, a harmonisation of the terminology used for the description of the morphological effects seen after chemical exposure has become necessary.

Conclusion

For this end, the present communication provides an overview of both common and selected more specific morphological effects seen in zebrafish embryos after exposure to a wide variety of chemical substances together with suggestions for a harmonised nomenclature.
Polychlorinated biphenyls and organochlorine pesticides in water and sediment from Volturno River, Southern Italy: occurrence, distribution and risk assessment
Authors: Montuori Paolo , De Rosa Elvira , Sarnacchiaro Pasquale , Di Duca Fabiana , Provvisiero Donatella Paola , Nardone Antonio , Triassi Maria
Published: September 2020

Background

To assess the Volturno River pollution and its environmental impact on the Tyrrhenian Sea (Central Mediterranean Sea) caused by Polychlorinated biphenyls (PCBs) and Organochlorine pesticides (OCPs), 32 PCBs and aldrin, α-BHC, β-BHC, δ-BHC, γ-BHC (lindane), 4,4′- DDD, 4,4′-DDE, 4,4′-DDT, dieldrin, endosulfan I, endosulfan II, endosulfan sulphate, endrin, heptachlor, heptachlor epoxide (isomer B) and methoxychlor have been selected and were analyzed in three different phases: dissolved phase (DP), suspended particulate matter (SPM) and sediments. PCBs and OCPs were extracted using an SPE column for the DP and glass fiber filter for SPM and sediment samples. Cleaned extracts were analyzed by gas chromatography with electron capture detector (ECD) and mass spectrometry (MS) detector for PCBs and OCPs, respectively.

Results

Pollutants discharges of PCBs and OCPs into the sea were calculated in about 106.9 kg year−1 (87.1 kg year−1 of PCBs and 19.7 kg year−1 of OCPs), showing that this river could be a major source of PCBs and OCPs pollution to the Central Mediterranean Sea. Total concentrations of PCBs ranged from 4.1 to 48.0 ng L−1 in water (sum of DP and SPM) and from 4.3 to 64.3 ng g−1 in sediment samples. The concentrations of total organochlorine pesticides (OCPs) obtained in water (sum of DP and SPM) ranged from 0.93 to 8.66 ng L−1 and from 0.52 to 9.89 ng g−1 in sediment samples. Principal component analysis shows that all PCB compounds are more likely to come from surface runoff than an atmospheric deposition.

Conclusion

The data show that higher levels of PCBs and OCPs were found in sediment samples than in DP and SPM samples, which are an indication of no fresh inputs of these compounds. Based on our results, unintentionally produced PCBs by industrial processes (and other processes) were considered to be the main sources of PCBs in Volturno River and Estuary sediments. Considering the Sediment Quality Guidelines (SQGs), the Ecological Risk Index (ERI), the Risk Quotient (RQ) and the USEPA Environmental Quality Standards (EQS), the Volturno River and its Estuary would be considered an area in which the integrity is possibly at risk.
Application of the common base method to regression and analysis of covariance (ANCOVA) in qPCR experiments and subsequent relative expression calculation
Authors: Ganger Michael T. , Dietz Geoffrey D. , Headley Patrick , Ewing Sarah J.
Published: September 2020

Background

Quantitative polymerase chain reaction (qPCR) is the technique of choice for quantifying gene expression. While the technique itself is well established, approaches for the analysis of qPCR data continue to improve.

Results

Here we expand on the common base method to develop procedures for testing linear relationships between gene expression and either a measured dependent variable, independent variable, or expression of another gene. We further develop functions relating variables to a relative expression value and develop calculations for determination of associated confidence intervals.

Conclusions

Traditional qPCR analysis methods typically rely on paired designs. The common base method does not require such pairing of samples. It is therefore applicable to other designs within the general linear model such as linear regression and analysis of covariance. The methodology presented here is also simple enough to be performed using basic spreadsheet software.
A comparative genomics study of neuropeptide genes in the cnidarian subclasses Hexacorallia and Ceriantharia
Authors: Koch Thomas L. , Grimmelikhuijzen Cornelis J. P.
Published: September 2020

Background

Nervous systems originated before the split of Proto- and Deuterostomia, more than 600 million years ago. Four animal phyla (Cnidaria, Placozoa, Ctenophora, Porifera) diverged before this split and studying these phyla could give us important information on the evolution of the nervous system. Here, we have annotated the neuropeptide preprohormone genes of twenty species belonging to the subclass Hexacorallia or Ceriantharia (Anthozoa: Cnidaria), using thirty-seven publicly accessible genome or transcriptome databases. Studying hexacorals is important, because they are versatile laboratory models for development (e.g., Nematostella vectensis) and symbiosis (e.g., Exaiptasia diaphana) and also are prominent reef-builders.

Results

We found that each hexacoral or ceriantharian species contains five to ten neuropeptide preprohormone genes. Many of these preprohormones contain multiple copies of immature neuropeptides, which can be up to 50 copies of identical or similar neuropeptide sequences. We also discovered preprohormones that only contained one neuropeptide sequence positioned directly after the signal sequence. Examples of them are neuropeptides that terminate with the sequence RWamide (the Antho-RWamides). Most neuropeptide sequences are N-terminally protected by pyroglutamyl (pQ) or one or more prolyl residues, while they are C-terminally protected by an amide group. Previously, we isolated and sequenced small neuropeptides from hexacorals that were N-terminally protected by an unusual L-3-phenyllactyl group. In our current analysis, we found that these N-phenyllactyl-peptides are derived from N-phenylalanyl-peptides located directly after the signal sequence of the preprohormone. The N-phenyllactyl- peptides appear to be confined to the hexacorallian order Actiniaria and do not occur in other cnidarians. On the other hand, (1) the neuropeptide Antho-RFamide (pQGRFamide); (2) peptides with the C-terminal sequence GLWamide; and (3) tetrapeptides with the X1PRX2amide consensus sequence (most frequently GPRGamide) are ubiquitous in Hexacorallia.

Conclusions

We found GRFamide, GLWamide, and X1PRX2amide peptides in all tested Hexacorallia. Previously, we discovered these three neuropeptide classes also in Cubozoa, Scyphozoa, and Staurozoa, indicating that these neuropeptides originated in the common cnidarian ancestor and are evolutionarily ancient. In addition to these ubiquitous neuropeptides, other neuropeptides appear to be confined to specific cnidarian orders or subclasses.
Flagella by numbers: comparative genomic analysis of the supernumerary flagellar systems among the Enterobacterales
Authors: De Maayer Pieter , Pillay Talia , Coutinho Teresa A.
Published: September 2020

Background

Flagellar motility is an efficient means of movement that allows bacteria to successfully colonize and compete with other microorganisms within their respective environments. The production and functioning of flagella is highly energy intensive and therefore flagellar motility is a tightly regulated process. Despite this, some bacteria have been observed to possess multiple flagellar systems which allow distinct forms of motility.

Results

Comparative genomic analyses showed that, in addition to the previously identified primary peritrichous (flag-1) and secondary, lateral (flag-2) flagellar loci, three novel types of flagellar loci, varying in both gene content and gene order, are encoded on the genomes of members of the order Enterobacterales. The flag-3 and flag-4 loci encode predicted peritrichous flagellar systems while the flag-5 locus encodes a polar flagellum. In total, 798/4028 (~ 20%) of the studied taxa incorporate dual flagellar systems, while nineteen taxa incorporate three distinct flagellar loci. Phylogenetic analyses indicate the complex evolutionary histories of the flagellar systems among the Enterobacterales.

Conclusions

Supernumerary flagellar loci are relatively common features across a broad taxonomic spectrum in the order Enterobacterales. Here, we report the occurrence of five (flag-1 to flag-5) flagellar loci on the genomes of enterobacterial taxa, as well as the occurrence of three flagellar systems in select members of the Enterobacterales. Considering the energetic burden of maintaining and operating multiple flagellar systems, they are likely to play a role in the ecological success of members of this family and we postulate on their potential biological functions.
In vitro resynthesis of lichenization reveals the genetic background of symbiosis-specific fungal-algal interaction in Usnea hakonensis
Authors: Kono Mieko , Kon Yoshiaki , Ohmura Yoshihito , Satta Yoko , Terai Yohey
Published: September 2020

Background

Symbiosis is central to ecosystems and has been an important driving force of the diversity of life. Close and long-term interactions are known to develop cooperative molecular mechanisms between the symbiotic partners and have often given them new functions as symbiotic entities. In lichen symbiosis, mutualistic relationships between lichen-forming fungi and algae and/or cyanobacteria produce unique features that make lichens adaptive to a wide range of environments. Although the morphological, physiological, and ecological uniqueness of lichens has been described for more than a century, the genetic mechanisms underlying this symbiosis are still poorly known.

Results

This study investigated the fungal-algal interaction specific to the lichen symbiosis using Usnea hakonensis as a model system. The whole genome of U. hakonensis, the fungal partner, was sequenced by using a culture isolated from a natural lichen thallus. Isolated cultures of the fungal and the algal partners were co-cultured in vitro for 3 months, and thalli were successfully resynthesized as visible protrusions. Transcriptomes of resynthesized and natural thalli (symbiotic states) were compared to that of isolated cultures (non-symbiotic state). Sets of fungal and algal genes up-regulated in both symbiotic states were identified as symbiosis-related genes.

Conclusion

From predicted functions of these genes, we identified genetic association with two key features fundamental to the symbiotic lifestyle in lichens. The first is establishment of a fungal symbiotic interface: (a) modification of cell walls at fungal-algal contact sites; and (b) production of a hydrophobic layer that ensheaths fungal and algal cells;. The second is symbiosis-specific nutrient flow: (a) the algal supply of photosynthetic product to the fungus; and (b) the fungal supply of phosphorous and nitrogen compounds to the alga. Since both features are widespread among lichens, our result may indicate important facets of the genetic basis of the lichen symbiosis.
Development of a SNP linkage map and genome-wide association study for resistance to Aeromonas hydrophila in pacu (Piaractus mesopotamicus)
Authors: Mastrochirico-Filho Vito A. , Borges Carolina H. S. , Freitas Milena V. , Ariede Raquel B. , Pilarski Fabiana , Utsunomia Ricardo , Carvalheiro Roberto , Gutierrez Alejandro P. , Peñaloza Carolina , Yáñez José M. , Houston Ross D. , Hashimoto Diogo T.
Published: September 2020

Background

Pacu (Piaractus mesopotamicus) is one of the most important Neotropical aquaculture species from South America. Disease outbreaks caused by Aeromonas hydrophila infection have been considered significant contributors to the declining levels of pacu production. The current implementation of genomic selection for disease resistance has been adopted as a powerful strategy for improvement in fish species. This study aimed to investigate the genetic architecture of resistance to A. hydrophila in pacu via Genome-Wide Association Study (GWAS), the identification of suggestive Quantitative Trait Loci (QTLs) and putative genes associated with this trait. The genetic data were obtained from 381 juvenile individuals belonging to 14 full-sibling families. An experimental challenge was performed to gain access to the levels of genetic variation for resistance against the bacteria using the following trait definitions: binary test survival (TS) and time of death (TD).

Results

The analyses of genetic parameters estimated moderate heritability (h2) for both resistance traits: 0.20 (± 0.09) for TS and 0.35 (± 0.15) for TD. A linkage map for pacu was developed to enable the GWAS, resulting in 27 linkage groups (LGs) with 17,453 mapped Single Nucleotide Polymorphisms (SNPs). The length of the LGs varied from 79.95 (LG14) to 137.01 (LG1) cM, with a total map length of 2755.60 cM. GWAS identified 22 putative QTLs associated to A. hydrophila resistance. They were distributed into 17 LGs, and were considered suggestive genomic regions explaining > 1% of the additive genetic variance (AGV) for the trait. Several candidate genes related to immune response were located close to the suggestive QTLs, such as tbk1, trim16, Il12rb2 and lyz2.

Conclusion

This study describes the development of the first medium density linkage map for pacu, which will be used as a framework to study relevant traits to the production of this species. In addition, the resistance to A. hydrophila was found to be moderately heritable but with a polygenic architecture suggesting that genomic selection, instead of marker assisted selection, might be useful for efficiently improving resistance to one of the most problematic diseases that affects the South American aquaculture.
Primary care physician perspectives on barriers to diagnosing axial Spondyloarthritis: a qualitative study
Authors: Lapane Kate L. , Khan Sara , Shridharmurthy Divya , Beccia Ariel , Dubé Catherine , Yi Esther , Kay Jonathan , Liu Shao-Hsien
Published: September 2020

Background

The average delay in diagnosis for patients with axial spondyloarthritis (axSpA) is 7 to 10 years. Factors that contribute to this delay are multifactorial and include the lack of diagnostic criteria (although classification criteria exist) for axSpA and the difficulty in distinguishing inflammatory back pain, a key symptom of axSpA, from other highly prevalent forms of low back pain. We sought to describe reasons for diagnostic delay for axSpA provided by primary care physicians.

Methods

We conducted a qualitative research study which included 18 US primary care physicians, balanced by gender. Physicians provided informed consent to participate in an in-depth interview (< 60 min), conducted in person (n = 3) or over the phone (n = 15), in 2019. The analysis focuses on thoughts about factors contributing to diagnostic delay in axSpA.

Results

Physicians noted that the disease characteristics contributing to diagnostic delay include: back pain is common and axSpA is less prevalent, slow progression of axSpA, intermittent nature of axSpA pain, and in the absence of abnormal radiographs of the spine or sacroiliac joints, there is no definitive test for axSpA. Patient characteristics believed to contribute to diagnostic delay included having multiple conditions in need of attention, infrequent interactions with the health care system, and “doctor shopping.” Doctors noted that patients wait until the last moments of the clinical encounter to discuss back pain. Problematic physician characteristics included lack of rapport with patients, lack of setting appropriate expectations, and attribution of back pain to other factors. Structural/system issues included short appointments, lack of continuity of care, insufficient insurance coverage for tests, lack of back pain clinics, and a shortage of rheumatologists.

Conclusion

Primary care physicians agreed that lengthy axSpA diagnosis delays are challenging to address owing to the multifactorial causes (e.g., disease characteristics, patient characteristics, lack of definitive tests, system factors).