bluuebunny/crossref_metadata_2025 huggingface dataset:
Immunomagnetic Separation of Microorganisms with Iron Oxide Nanoparticles
Authors: Thomas Julian A. , Schnell Florian , Kaveh-Baghbaderani Yasmin , Berensmeier Sonja , Schwaminger Sebastian P.
Published: February 2020

The early detection of Legionella in water reservoirs, and the prevention of their often fatal diseases, requires the development of rapid and reliable detection processes. A method for the magnetic separation (MS) of Legionella pneumophila by superparamagnetic iron oxide nanoparticles is developed, which represents the basis for future bacteria detection kits. The focus lies on the separation process and the simplicity of using magnetic nanomaterials. Iron oxide nanoparticles are functionalized with epoxy groups and Legionella-specific antibodies are immobilized. The resulting complexes are characterized with infrared spectroscopy and tested for the specific separation and enrichment of the selected microorganisms. The cell-particle complexes can be isolated in a magnetic field and detected with conventional methods such as fluorescence detection. A nonspecific enrichment of bacteria is also possible by using bare iron oxide nanoparticles (BIONs), which we used as a reference to the nanoparticles with immobilized antibodies. Furthermore, the immunomagnetic separation can be applied for the detection of multiple other microorganisms and thus might pave the way for simpler bacterial diagnosis.
On Implementing Autonomic Systems with a Serverless Computing Approach: The Case of Self-Partitioning Cloud Caches
Authors: Boza Edwin F. , Andrade Xavier , Cedeno Jorge , Murillo Jorge , Aragon Harold , Abad Cristina L. , Abad Andres G.
Published: February 2020

The research community has made significant advances towards realizing self-tuning cloud caches; notwithstanding, existing products still require manual expert tuning to maximize performance. Cloud (software) caches are built to swiftly serve requests; thus, avoiding costly functionality additions not directly related to the request-serving control path is critical. We show that serverless computing cloud services can be leveraged to solve the complex optimization problems that arise during self-tuning loops and can be used to optimize cloud caches for free. To illustrate that our approach is feasible and useful, we implement SPREDS (Self-Partitioning REDiS), a modified version of Redis that optimizes memory management in the multi-instance Redis scenario. A cost analysis shows that the serverless computing approach can lead to significant cost savings: The cost of running the controller as a serverless microservice is 0.85% of the cost of the always-on alternative. Through this case study, we make a strong case for implementing the controller of autonomic systems using a serverless computing approach.
Unified Methodology to Identify the Potential Application of Seasonal Sorption Storage Technology
Authors: Frazzica Andrea , Brancato Vincenza , Dawoud Belal
Published: February 2020

In this study, the definition of a new methodology for a preliminary evaluation of the working boundary conditions under which a seasonal thermal energy storage (STES) system operates is described. The approach starts by considering the building features as well as the reference heating system in terms of solar thermal collectors’ technology, ambient heat sinks/source, and space heating distribution systems employed. Furthermore, it is based on a deep climatic analysis of the place where the STES needs to be installed, to identify both winter and summer operating conditions. In particular, the STES energy density is evaluated considering different space heating demands covered by the STES (ranging from 10% up to 60%). The obtained results demonstrate that this approach allows for the careful estimation of the achievable STES density, which is varies significantly both with the space heating coverage guaranteed by the STES as well as with the ambient heat source/sink that is employed in the system. This confirms the need for careful preliminary analysis to avoid the overestimation of the STES material volume. The proposed approach was then applied for different climatic conditions (e.g., Germany and Sweden) and the volume of one of the most attractive composite sorbent materials reported in the literature, i.e., multi-wall carbon nanotubes (MWCNT)-LiCl, using water as the working fluid, needed for covering the variable space heating demand in a Nearly Zero Energy Building (NZEB) was calculated. In the case of Swedish buildings, it ranges from about 3.5 m3 when 10% of the space heating demand is provided by the STES, up to 11.1 m3 when 30% of the space heating demand is provided by the STES.
Flash Characteristics and Precipitation Metrics of Western U.S. Lightning-Initiated Wildfires from 2017
Authors: MacNamara Brittany R. , Schultz Christopher J. , Fuelberg Henry E.
Published: February 2020

This study examines 95 lightning-initiated wildfires and 1170 lightning flashes in the western United States between May and October 2017 to characterize lightning and precipitation rates and totals near the time of ignition. Eighty-nine percent of the wildfires examined were initiated by negative cloud-to-ground (CG) lightning flashes, and 66% of those fire starts were due to single stroke flashes. Average flash density at the fire locations was 1.1 fl km−2. The fire start locations were a median distance of 5.3 km away from the maximum flash and stroke densities in the 400 km2 area surrounding the fire start location. Fire start locations were observed to have a smaller 2-min precipitation rate and 24-h total rainfall than non-fire start locations. The median 2-min rainfall rate for fire-starting (FS) flash locations was 1.7 mm h−1, while the median for non-fire-starting (NFS) flash locations was 4.7 mm h−1. The median total 24-h precipitation value for FS flash locations was 2.9 mm, while NFS flash locations exhibited a median of 8.6 mm. Wilcoxon–Mann–Whitney rank sum testing revealed statistically different Z-Scores/p-values for the FS and NFS flash populations. These values were −5.578/1.21 × 10−8 and −7.176/3.58 × 10−13 for the 2-min precipitation rate and 24-h total rainfall, respectively. Additionally, 24-h and 2-min precipitation rates were statistically significantly greater for holdover versus non-holdover fire events. The median distances between the fire start location and greatest 2-min precipitation rate and greatest 24-h precipitation total were 7.4 and 10.1 km, respectively.
Mutation-Dependent Pathomechanisms Determine the Phenotype in the Bestrophinopathies
Authors: Nachtigal Anna-Lena , Milenkovic Andrea , Brandl Caroline , Schulz Heidi L. , Duerr Lisa M. J. , Lang Gabriele E. , Reiff Charlotte , Herrmann Philipp , Kellner Ulrich , Weber Bernhard H.F.
Published: February 2020

Best vitelliform macular dystrophy (BD), autosomal dominant vitreoretinochoroidopathy (ADVIRC), and the autosomal recessive bestrophinopathy (ARB), together known as the bestrophinopathies, are caused by mutations in the bestrophin-1 (BEST1) gene affecting anion transport through the plasma membrane of the retinal pigment epithelium (RPE). To date, while no treatment exists a better understanding of BEST1-related pathogenesis may help to define therapeutic targets. Here, we systematically characterize functional consequences of mutant BEST1 in thirteen RPE patient cell lines differentiated from human induced pluripotent stem cells (hiPSCs). Both BD and ARB hiPSC-RPEs display a strong reduction of BEST1-mediated anion transport function compared to control, while ADVIRC mutations trigger an increased anion permeability suggesting a stabilized open state condition of channel gating. Furthermore, BD and ARB hiPSC-RPEs differ by the degree of mutant protein turnover and by the site of subcellular protein quality control with adverse effects on lysosomal pH only in the BD-related cell lines. The latter finding is consistent with an altered processing of catalytic enzymes in the lysosomes. The present study provides a deeper insight into distinct molecular mechanisms of the three bestrophinopathies facilitating functional categorization of the more than 300 known BEST1 mutations that result into the distinct retinal phenotypes.
In-Depth Understanding of Camellia oleifera Self-Incompatibility by Comparative Transcriptome, Proteome and Metabolome
Authors: Zhou Junqin , Lu Mengqi , Yu Shushu , Liu Yiyao , Yang Jin , Tan Xiaofeng
Published: February 2020

Oil-tea tree (Camellia oleifera) is the most important edible oil tree species in China with late-acting self-incompatibility (LSI) properties. The mechanism of LSI is uncertain, which seriously hinders the research on its genetic characteristics, construction of genetic map, selection of cross breeding parents and cultivar arrangement. To gain insights into the LSI mechanism, we performed cytological, transcriptomic, proteomic and metabolomic studies on self- and cross-pollinated pistils. The studies identified 166,591 transcripts, 6851 proteins and 6455 metabolites. Transcriptomic analysis revealed 1197 differentially expressed transcripts between self- and cross-pollinated pistils and 47 programmed cell death (PCD)-control transcripts. Trend analysis by Pearson correlation categorized nine trend graphs linked to 226 differentially expressed proteins and 38 differentially expressed metabolites. Functional enrichment analysis revealed that the LSI was closely associated with PCD-related genes, mitogen-activated protein kinase (MAPK) signaling pathway, plant hormone signal transduction, ATP-binding cassette (ABC) transporters and ubiquitin-mediated proteolysis. These particular trends in transcripts, proteins and metabolites suggested the involvement of PCD in LSI. The results provide a solid genetic foundation for elucidating the regulatory network of PCD-mediated self-incompatibility in C. oleifera.
Inherited Eye Diseases with Retinal Manifestations through the Eyes of Homeobox Genes
Authors: Markitantova Yuliya , Simirskii Vladimir
Published: February 2020

Retinal development is under the coordinated control of overlapping networks of signaling pathways and transcription factors. The paper was conceived as a review of the data and ideas that have been formed to date on homeobox genes mutations that lead to the disruption of eye organogenesis and result in inherited eye/retinal diseases. Many of these diseases are part of the same clinical spectrum and have high genetic heterogeneity with already identified associated genes. We summarize the known key regulators of eye development, with a focus on the homeobox genes associated with monogenic eye diseases showing retinal manifestations. Recent advances in the field of genetics and high-throughput next-generation sequencing technologies, including single-cell transcriptome analysis have allowed for deepening of knowledge of the genetic basis of inherited retinal diseases (IRDs), as well as improve their diagnostics. We highlight some promising avenues of research involving molecular-genetic and cell-technology approaches that can be effective for IRDs therapy. The most promising neuroprotective strategies are aimed at mobilizing the endogenous cellular reserve of the retina.
Regulation of AQP4 in the Central Nervous System
Authors: Vandebroek Arno , Yasui Masato
Published: February 2020

Aquaporin-4 (AQP4) is the main water channel protein expressed in the central nervous system (CNS). AQP4 is densely expressed in astrocyte end-feet, and is an important factor in CNS water and potassium homeostasis. Changes in AQP4 activity and expression have been implicated in several CNS disorders, including (but not limited to) epilepsy, edema, stroke, and glioblastoma. For this reason, many studies have been done to understand the various ways in which AQP4 is regulated endogenously, and could be regulated pharmaceutically. In particular, four regulatory methods have been thoroughly studied; regulation of gene expression via microRNAs, regulation of AQP4 channel gating/trafficking via phosphorylation, regulation of water permeability using heavy metal ions, and regulation of water permeability using small molecule inhibitors. A major challenge when studying AQP4 regulation is inter-method variability. A compound or phosphorylation which shows an inhibitory effect in vitro may show no effect in a different in vitro method, or even show an increase in AQP4 expression in vivo. Although a large amount of variability exists between in vitro methods, some microRNAs, heavy metal ions, and two small molecule inhibitors, acetazolamide and TGN-020, have shown promise in the field of AQP4 regulation.
The Synergistic Effects of Sio2 Nanoparticles and Organic Photostabilizers for Enhanced Weathering Resistance of Acrylic Polyurethane Coating
Authors: Nguyen Thien Vuong , Nguyen Tuan Anh , Nguyen Thi Hau
Published: February 2020

This study aims to evaluate the synergical effects of SiO2 nanoparticles (nano-SiO2) and organic photostabilizers (Tinuvin 384 (T384) and Tinuvin 292 (T292)) on the weathering resistance of acrylic polyurethane coating. Data obtained from infrared (IR), field emission scanning electron microscopy (FESEM), and weight loss of coatings (before and after aging test), suggest that the SiO2 nanoparticles play a dual role, as both reinforcer and UV absorber, thus improving effectively both the mechanical properties and the weathering resistance of polyurethane acrylic coatings. The nanocomposite coating containing 2 wt % nano-SiO2, 2 wt % T384, and 1 wt % T292 exhibits excellent weathering and abrasion resistances, offering a durable outdoor application.
New Thiodiketopiperazine and 3,4-Dihydroisocoumarin Derivatives from the Marine-Derived Fungus Aspergillus terreus
Authors: Wu Jing-Shuai , Shi Xiao-Hui , Yao Guang-Shan , Shao Chang-Lun , Fu Xiu-Mei , Zhang Xiu-Li , Guan Hua-Shi , Wang Chang-Yun
Published: February 2020

Aspergillus terreus has been reported to produce many secondary metabolites that exhibit potential bioactivities, such as antibiotic, hypoglycemic, and lipid-lowering activities. In the present study, two new thiodiketopiperazines, emestrins L (1) and M (2), together with five known analogues (3–7), and five known dihydroisocoumarins (8–12), were obtained from the marine-derived fungus Aspergillus terreus RA2905. The structures of the new compounds were elucidated by analysis of the comprehensive spectroscopic data, including high-resolution electrospray ionization mass spectrometry (HRESIMS), one-dimensional (1D) and two-dimensional (2D) nuclear magnetic resonance (NMR), and electronic circular dichroism (ECD) data. This is the first time that the spectroscopic data of compounds 3, 8, and 9 have been reported. Compound 3 displayed antibacterial activity against Pseudomonas aeruginosa (minimum inhibitory concentration (MIC) = 32 μg/mL) and antifungal activity against Candida albicans (MIC = 32 μg/mL). In addition, compound 3 exhibited an inhibitory effect on protein tyrosine phosphatase 1 B (PTP1B), an important hypoglycemic target, with an inhibitory concentration (IC)50 value of 12.25 μM.
Iranian Medicinal Plants: From Ethnomedicine to Actual Studies
Authors: Buso Piergiacomo , Manfredini Stefano , Reza Ahmadi-Ashtiani Hamid , Sciabica Sabrina , Buzzi Raissa , Vertuani Silvia , Baldisserotto Anna
Published: February 2020

Iran has a rich and diverse cultural heritage, consisting of a complex traditional medicine deeply rooted in the history of the territory that goes back to the Assyrian and Babylonian civilizations. The ethnomedical practices that can be identifiable nowadays derive from the experience of local people who have developed remedies against a wide range of diseases handing down the knowledge from generation to generation over the millennia. Traditional medicine practices represent an important source of inspiration in the process of the development of new drugs and therapeutic strategies. In this context, it is useful to determine the state of the art of ethnomedical studies, concerning the Iranian territory, and of scientific studies on plants used in traditional Iranian medicine. Data regarding 245 plants used in Iranian ethnomedical practices and scientific studies conducted on 89 plants collected in the Iranian territory have been reported. All of the scientific studies here reported draw inspiration from traditional medicine. The World Health Organization (WHO) has repeatedly called for an intensification of the scientific validation processes of traditional medicines intended as an important contribution to public health in various parts of the world. The process of study and validation of Iranian ethnomedical practices appears to be at an early stage.
Bersavine: A Novel Bisbenzylisoquinoline Alkaloid with Cytotoxic, Antiproliferative and Apoptosis-Inducing Effects on Human Leukemic Cells
Authors: Koutova Darja , Kulhava Monika , Havelek Radim , Majorosova Martina , Královec Karel , Habartova Klara , Hošťálková Anna , Opletal Lubomír , Cahlikova Lucie , Řezáčová Martina
Published: February 2020

Bersavine is the new bisbenzylisoquinoline alkaloid isolated from the Berberis vulgaris L. (Berberidaceae) plant. The results of cytotoxicity screening 48 h post-treatment showed that bersavine considerably inhibits the proliferation and viability of leukemic (Jurkat, MOLT-4), colon (HT-29), cervix (HeLa) and breast (MCF-7) cancer cells with IC50 values ranging from 8.1 to 11 µM. The viability and proliferation of leukemic Jurkat and MOLT-4 cells were decreased after bersavine treatment in a time- and dose-dependent manner. Bersavine manifested concentration-dependent antiproliferative activity in human lung, breast, ovarian and hepatocellular carcinoma cell lines using a xCELLigence assay. Significantly higher percentages of MOLT-4 cells exposed to bersavine at 20 µM for 24 h were arrested in the G1 phase of the cell cycle using the flow cytometry method. The higher percentage of apoptotic cells was measured after 24 h of bersavine treatment. The upregulation of p53 phosphorylated on Ser392 was detected during the progression of MOLT-4 cell apoptosis. Mechanistically, bersavine-induced apoptosis is an effect of increased activity of caspases, while reduced proliferation seems dependent on increased Chk1 Ser345 phosphorylation and decreased Rb Ser807/811 phosphorylation in human leukemic cells.
Glycosaminoglycan-Inspired Biomaterials for the Development of Bioactive Hydrogel Networks
Authors: Neves Mariana I. , Araújo Marco , Moroni Lorenzo , da Silva Ricardo M.P. , Barrias Cristina C.
Published: February 2020

Glycosaminoglycans (GAG) are long, linear polysaccharides that display a wide range of relevant biological roles. Particularly, in the extracellular matrix (ECM) GAG specifically interact with other biological molecules, such as growth factors, protecting them from proteolysis or inhibiting factors. Additionally, ECM GAG are partially responsible for the mechanical stability of tissues due to their capacity to retain high amounts of water, enabling hydration of the ECM and rendering it resistant to compressive forces. In this review, the use of GAG for developing hydrogel networks with improved biological activity and/or mechanical properties is discussed. Greater focus is given to strategies involving the production of hydrogels that are composed of GAG alone or in combination with other materials. Additionally, approaches used to introduce GAG-inspired features in biomaterials of different sources will also be presented.
Design and Construction of a Focused DNA-Encoded Library for Multivalent Chromatin Reader Proteins
Authors: Rectenwald Justin M. , Guduru Shiva Krishna Reddy , Dang Zhao , Collins Leonard B. , Liao Yi-En , Norris-Drouin Jacqueline L. , Cholensky Stephanie H. , Kaufmann Kyle W. , Hammond Scott M. , Kireev Dmitri B. , Frye Stephen V. , Pearce Kenneth H.
Published: February 2020

Chromatin structure and function, and consequently cellular phenotype, is regulated in part by a network of chromatin-modifying enzymes that place post-translational modifications (PTMs) on histone tails. These marks serve as recruitment sites for other chromatin regulatory complexes that ‘read’ these PTMs. High-quality chemical probes that can block reader functions of proteins involved in chromatin regulation are important tools to improve our understanding of pathways involved in chromatin dynamics. Insight into the intricate system of chromatin PTMs and their context within the epigenome is also therapeutically important as misregulation of this complex system is implicated in numerous human diseases. Using computational methods, along with structure-based knowledge, we have designed and constructed a focused DNA-Encoded Library (DEL) containing approximately 60,000 compounds targeting bi-valent methyl-lysine (Kme) reader domains. Additionally, we have constructed DNA-barcoded control compounds to allow optimization of selection conditions using a model Kme reader domain. We anticipate that this target-class focused approach will serve as a new method for rapid discovery of inhibitors for multivalent chromatin reader domains.
Design, Synthesis, and In Vitro Evaluation of Hydroxybenzimidazole-Donepezil Analogues as Multitarget-Directed Ligands for the Treatment of Alzheimer’s Disease
Authors: Chaves Sílvia , Resta Simonetta , Rinaldo Federica , Costa Marina , Josselin Romane , Gwizdala Karolina , Piemontese Luca , Capriati Vito , Pereira-Santos A. Raquel , Cardoso Sandra M. , Santos M. Amélia
Published: February 2020

A series of multi-target-directed ligands (MTDLs), obtained by attachment of a hydroxyphenylbenzimidazole (BIM) unit to donepezil (DNP) active mimetic moiety (benzyl-piperidine/-piperazine) was designed, synthesized, and evaluated as potential anti-Alzheimer’s disease (AD) drugs in terms of biological activity (inhibition of acetylcholinesterase (AChE) and β–amyloid (Aβ) aggregation), metal chelation, and neuroprotection capacity. Among the DNP-BIM hybrids studied herein, the structural isomerization did not significantly improve the biological properties, while some substitutions, namely fluorine atom in each moiety or the methoxy group in the benzyl ring, evidenced higher cholinergic AChE activity. All the compounds are able to chelate Cu and Zn metal ions through their bidentate BIM moieties, but compound 5, containing a three-dentate chelating unit, is the strongest Cu(II) chelator. Concerning the viability on neuroblastoma cells, compounds 9 and 10 displayed the highest reduction of Aβ-induced cell toxicity. In silico calculations of some pharmacokinetic descriptors indicate that all the compounds but the nitro derivatives have good potential oral-bioavailability. Overall, it can be concluded that most of the studied DNP-BIM conjugates showed quite good anti-AD properties, therefore deserving to be considered in further studies with the aim of understanding and treating AD.
Synthesis and Antiproliferative Evaluation of Novel Longifolene-Derived Tetralone Derivatives Bearing 1,2,4-Triazole Moiety
Authors: Zhu Xia-Ping , Lin Gui-Shan , Duan Wen-Gui , Li Qing-Min , Li Fang-Yao , Lu Shun-Zhong
Published: February 2020

Seventeen novel 2-(5-amino-1-(substituted sulfonyl)-1H-1,2,4-triazol-3-ylthio)-6- isopropyl-4,4-dimethyl-3,4-dihydronaphthalen-1(2H)-one compounds were synthesized from the abundant and naturally renewable longifolene and their structures were confirmed by FT-IR, NMR, and ESI-MS. The in vitro cytotoxicity of the synthesized compounds was evaluated by standard MTT assay against five human cancer cell lines, i.e., T-24, MCF-7, HepG2, A549, and HT-29. As a result, compounds 6d, 6g, and 6h exhibited better and more broad-spectrum anticancer activity against almost all the tested cancer cell lines than that of the positive control, 5-FU. Some intriguing structure–activity relationships were found and are discussed herein by theoretical calculation.
Determination of Eight Coccidiostats in Eggs by Liquid–Liquid Extraction–Solid-Phase Extraction and Liquid Chromatography–Tandem Mass Spectrometry
Authors: Wang Bo , Liu Jianyu , Zhao Xia , Xie Kaizhou , Diao Zhixiang , Zhang Genxi , Zhang Tao , Dai Guojun
Published: February 2020

A method for the simultaneous determination of robenidine, halofuginone, lasalocid, monensin, nigericin, salinomycin, narasin, and maduramicin residues in eggs by liquid chromatography–tandem mass spectrometry (LC–MS/MS) was developed. The sample preparation method used a combination of liquid–liquid extraction (LLE) and solid-phase extraction (SPE) technology to extract and purify these target compounds from eggs. The target compounds were separated by gradient elution using high-performance liquid chromatography (HPLC) and ultra-performance liquid chromatography (UPLC). Tandem mass spectrometry was used to quantitatively and qualitatively analyze the target compounds via electrospray ionization (ESI+) and multiple reaction monitoring mode. The HPLC–MS/MS and UPLC–MS/MS methods were validated according to the requirements defined by the European Union and the Food and Drug Administration. The limits of detection and limits of quantification of the eight coccidiostats in eggs were 0.23–0.52 µg/kg and 0.82–1.73 µg/kg for HPLC–MS/MS, and 0.16-0.42 µg/kg and 0.81-1.25 µg/kg for UPLC–MS/MS, respectively. The eggs were spiked with four concentrations of the eight coccidiostats, and the HPLC–MS/MS and UPLC–MS/MS average recoveries were all higher than 71.69% and 72.26%, respectively. Compared with the HPLC–MS/MS method, utilizing UPLC–MS/MS had the advantages of low reagent consumption, a short detection time, and high recovery and precision. Finally, the HPLC–MS/MS and UPLC–MS/MS methods were successfully applied to detect eight coccidiostats in 40 eggs.
A New Positron Emission Tomography Probe for Orexin Receptors Neuroimaging
Authors: Bai Ping , Bai Sha , Placzek Michael S. , Lu Xiaoxia , Fiedler Stephanie A. , Ntaganda Brenda , Wey Hsiao-Ying , Wang Changning
Published: February 2020

The orexin receptor (OX) is critically involved in motivation and sleep−wake regulation and holds promising therapeutic potential in various mood disorders. To further investigate the role of orexin receptors (OXRs) in the living human brain and to evaluate the treatment potential of orexin-targeting therapeutics, we herein report a novel PET probe ([11C]CW24) for OXRs in the brain. CW24 has moderate binding affinity for OXRs (IC50 = 0.253 μM and 1.406 μM for OX1R and OX2R, respectively) and shows good selectivity to OXRs over 40 other central nervous system (CNS) targets. [11C]CW24 has high brain uptake in rodents and nonhuman primates, suitable metabolic stability, and appropriate distribution and pharmacokinetics for brain positron emission tomography (PET) imaging. [11C]CW24 warrants further evaluation as a PET imaging probe of OXRs in the brain.
Protective Effect of Genistein against Compound 48/80 Induced Anaphylactoid Shock via Inhibiting MAS Related G Protein-Coupled Receptor X2 (MRGPRX2)
Authors: Kumar Mukesh , Singh Kailash , Duraisamy Karthi , Allam Ahmed A. , Ajarem Jamaan , Kwok Chong CHOW Billy
Published: February 2020

Anaphylactoid shock is a fatal hypersensitivity response caused by non-IgE mediated mast cell activation. These reactions are mediated by a family of G protein-coupled receptors (GPCRs) known as Mas related GPCRX2 (MRGPRX2). Several US FDA approved drugs which are used in day to day life have been reported to cause anaphylactoid shock. Surprisingly, no therapeutic drugs are available which can directly target MRGPRX2 for treatment of anaphylactoid shock. Genistein is a non-steroidal polyphenol known for its diverse physiological and pharmacological activities. In recent studies, Genistein has been reported for its anti-inflammatory activity on mast cells. However, the effects and mechanistic pathways of Genistein on anaphylactoid reaction remain unknown. In the present study, we designed a battery of in-vitro, in-silico and in-vivo experiments to evaluate the anti-anaphylactoid activity of Genistein in order to understand the possible molecular mechanisms of its action. The in-vitro results demonstrated the inhibitory activity of Genistein on MRGPRX2 activation. Further, a mouse model of anaphylactoid shock was used to evaluate the inhibitory activity of Genistein on blood vessel leakage and hind paw edema. Taken together, our findings have demonstrated a therapeutic potential of Genistein as a lead compound in the treatment of anaphylactoid shock via MRGPRX2.
DNA-Binding and Anticancer Activity of Binuclear Gold(I) Alkynyl Complexes with a Phenanthrenyl Bridging Ligand
Authors: Alsaeedi Mona S. , Babgi Bandar A. , Hussien Mostafa A. , Abdellattif Magda H. , Humphrey Mark G.
Published: February 2020

3,6-Diethynyl-9,10-diethoxyphenanthrene (4) was synthesized from phenanthrene and employed in the synthesis of the binuclear gold(I) alkynyl complexes (R3P)Au(C≡C–3-[C14H6-9,10-diethoxy]-6–C≡C)Au(PR3) (R = Ph (5a), Cy (5b)). The diyne 4 and complexes 5a and 5b were characterized by NMR spectroscopy, mass spectrometry, and elemental analysis. UV-Vis spectroscopy studies of the metal complexes and precursor diyne show strong π → π* transitions in the near UV region that red shift by ca. 50 nm upon coordination at the gold centers. The emission spectrum of 4 shows an intense fluorescence band centered at 420 nm which red shifts, slightly upon coordination of 4 to gold. Binding studies of 4, 5a, and 5b against calf thymus DNA were carried out, revealing that 4, 5a, and 5b have ≥40% stronger binding affinities than the commonly used intercalating agent ethidium bromide. The molecular docking scores of 4, 5a, and 5b with B-DNA suggest a similar trend in behavior to that observed in the DNA-binding study. Unlike the ligand 4, promising anticancer properties for 5a and 5b were observed against several cell lines; the DNA binding capability of the precursor alkyne was maintained, and its anticancer efficacy enhanced by the gold centers. Such phenanthrenyl complexes could be promising candidates in certain biological applications because the two components (phenanthrenyl bridge and metal centers) can be altered independently to improve the targeting of the complex, as well as the biological and physicochemical properties.
Quantum Dot Labelling of Tepary Bean (Phaseolus acutifolius) Lectins by Microfluidics
Authors: Cervantes-Jiménez Ricardo , Sánchez-Segura Lino , Estrada-Martínez Laura Elena , Topete-Camacho Antonio , Mendiola-Olaya Elizabeth , Rosas-Escareño Abraham Noé , Saldaña-Gutiérrez Carlos , Figueroa-Cabañas Mónica Eugenia , Dena-Beltrán José Luis , Kuri-García Aarón , Blanco-Labra Alejandro , García-Gasca Teresa
Published: February 2020

Lectins are bioactive proteins with the ability to recognize cell membrane carbohydrates in a specific way. Diverse plant lectins have shown diagnostic and therapeutic potential against cancer, and their cytotoxicity against transformed cells is mediated through the induction of apoptosis. Previous works have determined the cytotoxic activity of a Tepary bean (Phaseolus acutifolius) lectin fraction (TBLF) and its anti-tumorigenic effect on colon cancer. In this work, lectins from the TBLF were additionally purified by ionic-exchange chromatography. Two peaks with agglutination activity were obtained: one of them was named TBL-IE2 and showed a single protein band in two-dimensional electrophoresis; this one was thus selected for coupling to quantum dot (QD) nanoparticles by microfluidics (TBL-IE2-QD). The microfluidic method led to low sample usage, and resulted in homogeneous complexes, whose visualization was achieved using multiphoton and transmission electron microscopy. The average particle size (380 nm) and the average zeta potential (−18.51 mV) were determined. The cytotoxicity of the TBL-IE2 and TBL-IE2-QD was assayed on HT-29 colon cancer cells, showing no differences between them (p ≤ 0.05), where the LC50 values were 1.0 × 10−3 and 1.7 × 10−3 mg/mL, respectively. The microfluidic technique allowed control of the coupling between the QD and the protein, substantially improving the labelling process, providing a rapid and efficient method that enabled the traceability of lectins. Future studies will focus on the potential use of the QD-labelled lectin to recognize tumor tissues.
Application of Halogen Bonding to Organocatalysis: A Theoretical Perspective
Authors: Yang Hui , Wong Ming Wah
Published: February 2020

The strong, specific, and directional halogen bond (XB) is an ideal supramolecular synthon in crystal engineering, as well as rational catalyst and drug design. These attributes attracted strong growing interest in halogen bonding in the past decade and led to a wide range of applications in materials, biological, and catalysis applications. Recently, various research groups exploited the XB mode of activation in designing halogen-based Lewis acids in effecting organic transformation, and there is continual growth in this promising area. In addition to the rapid advancements in methodology development, computational investigations are well suited for mechanistic understanding, rational XB catalyst design, and the study of intermediates that are unstable when observed experimentally. In this review, we highlight recent computational studies of XB organocatalytic reactions, which provide valuable insights into the XB mode of activation, competing reaction pathways, effects of solvent and counterions, and design of novel XB catalysts.
Formation of Fe Nanoparticles by Ion Implantation Technique for Catalytic Graphitization of a Phenolic Resin
Authors: Idesaki Akira , Yamamoto Shunya , Sugimoto Masaki , Yamaki Tetsuya , Maekawa Yasunari
Published: February 2020

Ion implantation technique was employed to introduce iron nanoparticles (Fe NPs) into a carbon precursor polymer with the aim of forming of a graphitic nanostructure through catalytic graphitization by the introduced Fe NPs. A phenolic resin was implanted by 100 keV Fe+ ions with ion fluence of 1 × 1014–1 × 1016 ions/cm2 at ambient temperature under vacuum, and subsequently heat-treated at 800 °C in a nitrogen gas atmosphere. It was found that the particle size of Fe NPs could be controlled in the range of 5–30 nm by the Fe+ ion fluence. Additionally, it was found that a nanosized turbostratic graphite structure with mean interlayer distance of 0.3531 nm, which is consisted of shell-like carbon layers and intricately distorted carbon layers, was formed around the Fe NPs. The ion implantation technique is one of the advantageous ways to introduce size-controlled fine metal NPs which are effective for the formation of graphitic nanostructure from a carbon precursor polymer.
In Situ Analysis of the Phase Transformation Kinetics in the β-Water-Quenched Ti-5Al-5Mo-5V-3Cr-1Zr Alloy during Ageing after Fast Heating
Authors: Paiotti Marcondes Guimarães Rafael , Callegari Bruna , Warchomicka Fernando , Aristizabal Katherine , Soldera Flavio , Mücklich Frank , Cavalcanti Pinto Haroldo
Published: February 2020

Thermal treatments are the main route to achieve improvements in mechanical properties of β-metastable titanium alloys developed for structural applications in automotive and aerospace industries. Therefore, it is of vital importance to determine phase transformation kinetics and mechanisms of nucleation and precipitation during heat treatment of these alloys. In this context, the present paper focuses on the assessment of solid-state transformations in a β-water-quenched Ti-5Al-5Mo-5V-3Cr-1Zr alloy during the early stages of ageing treatment at 500 °C. In situ tracking of transformations was performed using high-energy synchrotron X-ray diffraction. The transformation sequence β + ω → α + α”iso + β is proposed to take place during this stage. Results show that isothermal α” phase precipitates from ω and from spinodal decomposition domains of the β phase, whereas α nucleates from ω, β and also from α” with different morphologies. Isothermal α” is considered to be the regulator of transformation kinetics. Hardness measurements confirm the presence of ω, although this phase was not detected by X-ray diffraction during the in situ treatment.
A Dual-Attention Recurrent Neural Network Method for Deep Cone Thickener Underflow Concentration Prediction
Authors: Yuan Zhaolin , Hu Jinlong , Wu Di , Ban Xiaojuan
Published: February 2020

This paper focuses on the time series prediction problem for underflow concentration of deep cone thickener. It is commonly used in the industrial sedimentation process. In this paper, we introduce a dual attention neural network method to model both spatial and temporal features of the data collected from multiple sensors in the thickener to predict underflow concentration. The concentration is the key factor for future mining process. This model includes encoder and decoder. Their function is to capture spatial and temporal importance separately from input data, and output more accurate prediction. We also consider the domain knowledge in modeling process. Several supplementary constructed features are examined to enhance the final prediction accuracy in addition to the raw data from sensors. To test the feasibility and efficiency of this method, we select an industrial case based on Industrial Internet of Things (IIoT). This Tailings Thickener is from FLSmidth with multiple sensors. The comparative results support this method has favorable prediction accuracy, which is more than 10% lower than other time series prediction models in some common error indices. We also try to interpret our method with additional ablation experiments for different features and attention mechanisms. By employing mean absolute error index to evaluate the models, experimental result reports that enhanced features and dual-attention modules reduce error of fitting ~5% and ~11%, respectively.
The AIDSS Module for Data Acquisition in Crisis Situations and Environmental Protection
Authors: Krtalić Andrija , Bajić Milan , Ivelja Tamara , Racetin Ivan
Published: February 2020

The Toolbox implementation for removal of antipersonnel mines, submunitions and unexploded ordnance (TIRAMISU) Advanced Intelligence Decision Support System is an operational system proposed to Mine Action Centres worldwide for conducting non-technical surveys in humanitarian demining. The system consists of three modules, one of which is the module for data acquisition introduced and described in this study. The module has been designed, produced, improved, used and operationally tested and validated on several platforms (helicopters, remotely piloted aircraft systems (RPAS) and a blimp), with various sensors and acquisition units (Global Positioning System (GPS) and inertial measurement unit) in a variety of combinations for additional data acquisition from deep inside a suspected hazardous area. For the purposes of aerial data acquisition over a suspected hazardous area, the use of multiple sensors such as visible digital cameras and multi-spectral visible, near infrared (VNIR), hyperspectral VNIR and thermal infrared sensors are of benefit, because they display the scene in different ways. Off-the-shelf equipment and software were mostly used, but some specific equipment, such as sensor pods, was developed and also some software solutions for data acquisition and pre-processing (transforming hyperspectral line scanner data into hyperspectral images, and producing hyperspectral cubes). The technical stability and robustness of the module were confirmed by operationally testing and evaluating the systems on the aforementioned platforms and missions in several actual suspected hazardous areas in Croatia and Bosnia and Herzegovina, between 2001 and 2015.
Joint Dwell Time and Bandwidth Optimization for Multi-Target Tracking in Radar Network Based on Low Probability of Intercept
Authors: Ding Lintao , Shi Chenguang , Qiu Wei , Zhou Jianjiang
Published: February 2020

Radar network systems have been demonstrated to offer numerous advantages for target tracking. In this paper, a low probability of intercept (LPI)-based joint dwell time and bandwidth optimization strategy is proposed for multi-target tracking in a radar network. Since the Bayesian Cramer–Rao lower bound (BCRLB) provides a lower bound on parameter estimation, it can be utilized as the accuracy metric for target tracking. In this strategy, in order to improve the LPI performance of the radar network, the total dwell time consumption of the underlying system is minimized, while guaranteeing a predetermined tracking accuracy. There are two adaptable parameters in the optimization problem: one for dwell time, and the other for bandwidth allocation. Since the nonlinear programming-based genetic algorithm (NPGA) can solve the nonlinear problem well, we develop a method based upon NPGA to solve the resulting problem. The simulation results demonstrate that the proposed strategy has superiority over traditional algorithms, and can achieve a better LPI performance of this radar network.
Understanding the Washing Damage to Textile ECG Dry Skin Electrodes, Embroidered and Fabric-Based; set up of Equivalent Laboratory Tests
Authors: Zaman Shahood uz , Tao Xuyuan , Cochrane Cédric , Koncar Vladan
Published: February 2020

Reliability and washability are major hurdles facing the e-textile industry nowadays. The main fear behind the product’s rejection is the inability to ensure its projected life span. The durability of e-textiles is based on an approximate lifetime of both the electronics and textiles integrated into the product. A detailed analysis of the wash process and the possibility of predicting product behavior are key factors for new standards implementation. This manuscript is focused on the washability issues of different types of woven, knitted, and embroidered, textile-based ECG electrodes. These electrodes are used without the addition of any ionic gel to the skin to reduce impedance. They were subjected to up to 50 wash cycles with two different types of wash processes, and changes in surface resistance, as well as the quality of ECG waves, were observed To investigate the wash damages in detail, the proposed mechanical (Martindale and Pilling box) and chemical test methods were investigated. The electrodes which increased resistance after washing showed the same trend in the proposed test methods. Copper-based electrodes suffered the most severe damage and increased resistance, as was also visible in an SEM analysis. These proposed test methods can be used to predict robustness behavior without washing.
Outage Analysis of Parasitic Ambient Backscatter Communication in Decode-and-Forward Relay Networks with SWIPT
Authors: Tuo Yanhong , Zhang Chao
Published: February 2020

In this paper, we investigate the outage performance of simultaneous wireless information and power transfer (SWIPT) based Decode-and-Forward (DF) relay networks, where the relay needs to simultaneously forward information for two relaying links, primary relaying link and parasitic relaying link. The primary relaying link is the traditional source-relay-destination relay system. While in the parasitic relaying link, the parasitic source, i.e., Internet-of-Things (IoT) tag, is not connected to the stable power source and thus has to backscatter the signals from the primary source to convey its information. The relay not only harvests energy from Radio Frequency (RF) signals from both sources but also forwards messages to their corresponding destinations. The primary source and destination are unaware of the parasitic backscatter transmission, but the relay and parasitic destination can employ successive interference cancellation (SIC) detector to eliminate the interference from the primary link and detect the message from the parasitic source. In order to investigate the interplay between the primary and parasitic relaying links, the outage probabilities of both relaying links are derived. Besides, the effects of system parameters, i.e., power splitting coefficient, forwarding power allocation coefficient and backscatter reflection coefficient, on the system performance are discussed. Simulation results verify our theoretical analysis. In the meanwhile, it is revealed that the advised relaying system has far larger sum throughput than the one with only primary relaying link and the parasitic relaying link can gain considerable throughput at the cost of negligible degradation of primary throughput.
Benchmarking of Dedicated Hybrid Transmissions
Authors: Sieg Christian , Küçükay Ferit
Published: February 2020

For many manufacturers, hybridization represents an attractive solution for reducing the energy consumption of their vehicles. However, electrification offers a wide range of possibilities for implementing powertrain concepts. The concepts can differ regarding their mechanical complexity and the required power of the electrical machines. In this article, drive concepts that differ in their functionality and drive train topology are compared. Based on requirements for the C, D, and E segment, the mechanical and electrical effort of the concepts is analyzed. The results show that the mechanical effort in the C segment can be reduced as long as the electrical effort is increased. In case of higher vehicle segments, the electrical effort can increase considerably, making concepts with increased mechanical complexity more suitable. The driving performance and efficiency in hybrid operation are evaluated via simulation. The results show that the difference of acceleration times in hybrid operation between a charged and discharged battery is lower for mechanically complex concepts. At the same time, they achieve lower CO2 emissions. Therefore, these concepts represent a better compromise regarding performance and efficiency. Despite lower transmission efficiencies in hybrid operation, they achieve conversion qualities similar to simpler concepts and lower emissions with lower electrical effort.
Three new species of hawkweed (Hieracium; Asteraceae) from northern England
Authors: McCosh D.J. , Barlow D. , Burrow B. , Rich T.C.G.
Published: February 2020

Three new species of hawkweed are described in Hieracium section Stelligera from Northern England, based on the herbarium collection of Vincent Jones; Hieracium lacinifolium V. Jones, Hieracium obovatifolium V. Jones and Hieracium pseudosubcyaneum V. Jones.
Lectotypification and neotypification of some names in British Hieracium section Foliosa (Asteraceae)
Authors: Rich T.C.G.
Published: February 2020

Types are designated for 11 names in Hieracium section Foliosa (Fr.) Arv.-Touv. (Asteraceae). Lectotypes are designated for Hieracium aestivum subsp. subumbellatiforme Zahn, Hieracium corymbosum Fr. var. pycnotrichum W. R. Linton, Hieracium crocatum Fr. var. maritimum F. Hanb., Hieracium latobrigorum forma angustifolium Pugsley, Hieracium obesifolium Pugsley, Hieracium pseudamplidentatum var. stenophyllum Pugsley, Hieracium rigidum var. tavense Ley, Hieracium strictum var. amplidentatum F. Hanb. and Hieracium strictum var. subcrocatum E. F. Linton. Neotypes are designated for Hieracium inuloides subsp. strictiforme Zahn and Hieracium neocorymbosum var. crocatiforme Pugsley.
Efeito da Terapia-Espelho na função motora dos membros inferiores na doença de Machado-Joseph
Authors: Dias Miqueline Pivoto Faria , Calixto Júnior Ruanito , Silva Andreia Maria , Silva Adriana Teresa
Published: February 2020

Objetivo. O objetivo deste estudo foi relatar o caso de um pacien­te com doença de Machado-Joseph submetido à Terapia-Espelho (TE) em membros inferiores. Método. Trata-se de um relato de caso de um paciente do gênero masculino, 56 anos, com diagnós­tico de Doença Machado-Joseph. Os instrumentos utilizados para avaliação foram a Escala de equilíbrio Berg (EEB), teste de cami­nhada de 5 metros (C5M), teste de subir e descer escadas (TSE) e (TDE), teste de Time up-and-Go (TT) e eletromiografia de su­perfície durante a contração isométrica voluntária (CIV) bilateral­mente e simultaneamente dos músculos retofemural (RF) e tibial anterior (TA). Para a intervenção aplicou-se a TE associada a exer­cícios nos membros inferiores. Resultados. Houve aumento na EEB (p=0,05), redução do tempo para os testes C5M, TES, TED, TT e aumento da atividade eletromiográfica para os músculos RF e TA. Conclusão. Conclui-se que a TE pode contribuir para melho­ra funcional e aumento na atividade eletromiográfica neste paciente.