bluuebunny/crossref_metadata_2025 huggingface dataset:
Are Copper Nanoparticles Toxic to All Plants? A Case Study on Onion (Allium cepa L.)
Authors: Mottaleb Shady Abdel , Hassan Ahmed Z. A. , El-Bahbohy Reham , Mahmoud Abdel Wahab M.
Published: May 2021

Sandy soils with high alkalinity are characterized by low copper (Cu) contents that lead to many deficiency symptoms in plants. Cu deficiency in plants can be corrected using several cheap Cu sources. Nevertheless, the effects that novel sources, such as Cu nanoparticles (NPs), have on plants remain poorly studied. In the present work, we investigated the effect and efficiency of Cu supplementation to onion (Allium cepa L.) plants using Cu sulfate, chelate, or NPs, and compared their effects on bulb quality, yield, and contents of phytochemicals. Two successive seasons (2018/2019 and 2019/2020) of field experiments were conducted in newly reclaimed sandy soils, where plants were sprayed with either 10 ppm CuO NPs, 20 ppm CuSO4·5H2O, or 20 ppm of Cu chelates. Overall, Cu deficiency (control) resulted in a significant decrease in yield and all quality traits of onion plants. CuO NPs treatment significantly enhanced growth parameters, including plant height, number of leaves, fresh and dry weight, yield, and bulb quality, compared with Cu sulfate and chelates. This was also the case regarding chemical constituents such as macro- and micro-nutrients, total soluble solids, phytochemical compounds, vitamins, and amino acids. Although Cu sulfate is the cheapest form used for Cu supplementation, results of the present study suggest that CuO NPs was not only safe to use, but also was the treatment that led to the highest onion yield and quality.
Pharmacodynamic Evaluation of a Single Dose versus a 24-Hour Course of Multiple Doses of Cefazolin for Surgical Prophylaxis
Authors: Heffernan Aaron , Alawie Jowana , Wallis Steven C , Naicker Saiyuri , Adiraju Santosh , Roberts Jason A. , Sime Fekade Bruck
Published: May 2021

The optimal perioperative duration for the administration of cefazolin and other prophylactic antibiotics remains unclear. This study aimed to describe the pharmacodynamics of cefazolin for a single 2 g dose versus a 24 h course of a 2 g single dose plus a 1 g eight-hourly regimen against methicillin-susceptible Staphylococcus aureus. Static concentration time–kill assay and a dynamic in vitro hollow-fibre infection model simulating humanised plasma and interstitial fluid exposures of cefazolin were used to characterise the pharmacodynamics of prophylactic cefazolin regimens against methicillin-sensitive Staphylococcus aureus clinical isolates. The initial inoculum was 1 × 105 CFU/mL to mimic a high skin flora inoculum. The static time–kill study showed that increasing the cefazolin concentration above 1 mg/L (the MIC) did not increase the rate or the extent of bacterial killing. In the dynamic hollow-fibre model, both dosing regimens achieved similar bacterial killing (~3-log CFU/mL within 24 h). A single 2 g dose may be adequate when low bacterial burdens (~104 CFU/mL) are anticipated in an immunocompetent patient with normal pharmacokinetics.
Atypical Iron-Sulfur Cluster Binding, Redox Activity and Structural Properties of Chlamydomonas reinhardtii Glutaredoxin 2
Authors: Roret Thomas , Zhang Bo , Moseler Anna , Dhalleine Tiphaine , Gao Xing-Huang , Couturier Jérémy , Lemaire Stéphane D. , Didierjean Claude , Johnson Michael K. , Rouhier Nicolas
Published: May 2021

Glutaredoxins (GRXs) are thioredoxin superfamily members exhibiting thiol-disulfide oxidoreductase activity and/or iron-sulfur (Fe-S) cluster binding capacities. These properties are determined by specific structural factors. In this study, we examined the capacity of the class I Chlamydomonas reinhardtii GRX2 recombinant protein to catalyze both protein glutathionylation and deglutathionylation reactions using a redox sensitive fluorescent protein as a model protein substrate. We observed that the catalytic cysteine of the CPYC active site motif of GRX2 was sufficient for catalyzing both reactions in the presence of glutathione. Unexpectedly, spectroscopic characterization of the protein purified under anaerobiosis showed the presence of a [2Fe-2S] cluster despite having a presumably inadequate active site signature, based on past mutational analyses. The spectroscopic characterization of cysteine mutated variants together with modeling of the Fe–S cluster-bound GRX homodimer from the structure of an apo-GRX2 indicate the existence of an atypical Fe–S cluster environment and ligation mode. Overall, the results further delineate the biochemical and structural properties of conventional GRXs, pointing to the existence of multiple factors more complex than anticipated, sustaining the capacity of these proteins to bind Fe–S clusters.
Apple Pomace as a Source of Bioactive Polyphenol Compounds in Gluten-Free Breads
Authors: Gumul Dorota , Ziobro Rafał , Korus Jarosław , Kruczek Marek
Published: May 2021

Gluten-free products based on starch and hydrocolloids are deficient in nutrients and do not contain pro-health substances. Therefore, they should be enriched in raw materials naturally rich in antioxidants, especially if they are intended for celiac patients, prone to high oxidative stress. Apart from the traditionally used pseudo-cereals, seeds, vegetables and fruits, innovative substrates such as the by-product (especially in Poland) dry apple pomace could be applied. The study material consisted of gluten-free bread enriched with apple pomace. The content of individual polyphenols, the content of total polyphenol and flavonoids, and also the antioxidant potential of the bread were determined by the UPLC-PDA-MS/MS methods. It was observed that apple pomace was a natural concentrate of bioactive substances from the group of polyphenols. In summary, gluten-free bread with 5% content of apple pomace showed the highest organoleptic scores and contained high levels of phenolic compounds. The values of total phenolic content, and the amounts of flavonoids, phenolic acids and phloridzin in this bread were 2.5, 8, 4 and 21 times higher in comparison to control.
Effects of Antioxidant Vitamins, Curry Consumption, and Heavy Metal Levels on Metabolic Syndrome with Comorbidities: A Korean Community-Based Cross-Sectional Study
Authors: Duc Hai Nguyen , Oh Hojin , Kim Min-Sun
Published: May 2021

The burden of metabolic syndrome (MetS) has increased worldwide, especially during the COVID-19 pandemic, and this phenomenon is related to environmental, dietary, and lifestyle risk factors. We aimed to determine the association between the levels of serum heavy metals, hs-CRP, vitamins, and curry intake and to predict risks of MetS based on marginal effects. A data set of 60,256 Koreans aged ≥ 15 years between 2009 and 2017 was used to obtain information on sociodemographic, lifestyle, family history characteristics, MetS, food intake survey, and serum heavy metals. Daily intake of vitamins was measured by a one-day 24 h recall, and curry consumption was calculated using a food frequency questionnaire. Serum heavy metal levels were quantified by graphite furnace atomic absorption spectrometry and using a mercury analyzer. We found that vitamin B1, B2, B3, C, and A intakes were significantly lower in subjects with than without MetS. In contrast, serum levels of Pb, Hg, Cd, vitamin A, E, and hs-CRP were significantly higher in subjects with MetS. The risk of MetS was significantly lower for high curry consumers than low curry consumers (adjusted odds ratio 0.85, 95%CI 0.74–0.98). The risks of MetS were reduced by 12% and 1%, when vitamin B1 and C intakes increased by one mg, respectively, but were increased by 14%, 3%, and 9%, when serum levels of Pb, Hg, and hs-CRP increased by one unit. These results show that the potential health benefits resulting from vitamin and curry intakes could protect the public against the dual burden of communicable and non-communicable diseases. Further studies are required to reduce risk factors associated with serum heavy metal levels and to determine whether interactions between vitamin and curry consumption influence the presence of MetS.
Changes in Plasma Bioactive Lipids and Inflammatory Markers during a Half-Marathon in Trained Athletes
Authors: Gaggini Melania , Vassalle Cristina , Carli Fabrizia , Maltinti Maristella , Sabatino Laura , Buzzigoli Emma , Mastorci Francesca , Sbrana Francesco , Gastaldelli Amalia , Pingitore Alessandro
Published: May 2021

Background: Exercise may affect lipid profile which in turn is related to inflammation, although changes of ceramides, diacylglycerols-DAG and sphingomyelin-SM and their relationship with inflammatory parameters following a half-marathon have never been examined. Methods: Ceramides, DAG and SM, and markers of inflammation (soluble fractalkine-CX3CL1, vascular endothelial growth factor-VEGF, interleukin6-IL-6 and tumor necrosis factorα-TNFα) were evaluated in trained half-marathoners before, post-race (withdrawal within 20 min after the race end) and 24 h after. Results: IL-6 and CX3CL1 increased immediately after the race, returning to baseline after 24 h. Total ceramides and total DAG significantly decreased post-race. Several ceramide classes decreased after exercise, while only one of the DAG (36:3) changed significantly. Total SM and specific species did not significantly change. Conclusion: Some inflammatory parameters (IL-6 and CX3CL1) transiently increased after the race, and, being reversible, these changes might represent a physiological response to acute exercise rather than a damage-related response. The decrease of specific lipid classes, i.e., DAGs and ceramides, and the lack of their relationship with inflammatory parameters, suggest their involvement in beneficial training effects, opening promising research perspectives to identify additional mechanisms of aerobic exercise adaptation.
Combining High- and Low-Rate Geodetic Data Analysis for Unveiling Rapid Magma Transfer Feeding a Sequence of Violent Summit Paroxysms at Etna in Late 2015
Authors: Bonforte Alessandro , Cannavò Flavio , Gambino Salvatore , Guglielmino Francesco
Published: May 2021

We propose a multi-temporal-scale analysis of ground deformation data using both high-rate tilt and GNSS measurements and the DInSAR and daily GNSS solutions in order to investigate a sequence of four paroxysmal episodes of the Voragine crater occurring in December 2015 at Mt. Etna (Italy). The analysis aimed at inferring the magma sources feeding a sequence of very violent eruptions, in order to understand the dynamics and to image the shallow feeding system of the volcano that enabled such a rapid magma accumulation and discharge. The high-rate data allowed us to constrain the sources responsible for the fast and violent dynamics of each paroxysm, while the cumulated deformation measured by DInSAR and daily GNSS solutions, over a period of 12 days encompassing the entire eruptive sequence, also showed the deeper part of the source involved in the considered period, where magma was stored. We defined the dynamics and rates of the magma transfer, with a middle-depth storage of gas-rich magma that charges, more or less continuously, a shallower level where magma stops temporarily, accumulating pressure due to the gas exsolution. This machine-gun-like mechanism could represent a general conceptual model for similar events at Etna and at all volcanoes.
Audio, Image, Video, and Weather Datasets for Continuous Electronic Beehive Monitoring
Authors: Kulyukin Vladimir
Published: May 2021

In 2014, we designed and implemented BeePi, a multi-sensor electronic beehive monitoring system. Since then we have been using BeePi monitors deployed at different apiaries in northern Utah to design audio, image, and video processing algorithms to analyze forager traffic in the vicinity of Langstroth beehives. Since our first publication on BeePi in 2016, we have received multiple requests from researchers and practitioners for the datasets we have used in our research. The main objective of this article is to provide a comprehensive point of reference to the datasets that we have so far curated for our research. We hope that our datasets will provide stable performance benchmarks for continuous electronic beehive monitoring, help interested parties verify our findings and correct errors, and advance the state of the art in continuous electronic beehive monitoring and related areas of AI, machine learning, and data science.
Analysis of Different Stop-Jumping Strategies on the Biomechanical Changes in the Lower Limbs
Authors: Zhou Huiyu , Xu Datao , Chen Chaoyi , Ugbolue Ukadike Chris , Baker Julien S. , Gu Yaodong
Published: May 2021

The stop-jumping task is one of the most important technical actions in basketball. A previous study showed 70% probability of non-contact ACL injuries during stop-jumping tasks. Therefore, the present study aimed to investigate the differences in lower extremity biomechanical changes between the rear foot as the initial contact area to terminate the jump (SJR) and the fore foot as the initial contact area to also terminate the jump (SJF) during the horizontal landing during a stop-jumping phase. In total, 25 male amateur Ningbo University basketball athletes from China were recruited for this study. The participants were asked to jump vertically by using two different stop-jumping strategies. Kinematic and kinetics data were amassed during a stop-jumping task. Statistical parametric mapping (SPM) analysis was used to find the differences between SJR and SJF. Our results indicated that the change of different ankle range of motion caused significantly different values for knee angle (p < 0.001), velocity (p = 0.003) (p = 0.023) (p < 0.001), moment (p = 0.04) (p < 0.001), (p = 0.036) and power (p = 0.015) (p < 0.001) during the stop-jumping phase and the horizontal landing phase. The same biomechanical parameters of the hip joint were also significantly different for hip angle (p < 0.001), moment (p = 0.012) (p < 0.001) (p < 0.001), and power (p = 0.01) (p < 0.001) (p < 0.001). These findings indicate that altering the primary contact at the ankle angle might effectively reduce the risk of a knee injury.
Identification of Existing Challenges and Future Trends for the Utilization of Ammonia-Water Absorption–Compression Heat Pumps at High Temperature Operation
Authors: Ahrens Marcel Ulrich , Loth Maximilian , Tolstorebrov Ignat , Hafner Armin , Kabelac Stephan , Wang Ruzhu , Eikevik Trygve Magne
Published: May 2021

Decarbonization of the industrial sector is one of the most important keys to reducing global warming. Energy demands and associated emissions in the industrial sector are continuously increasing. The utilization of high temperature heat pumps (HTHPs) operating with natural fluids presents an environmentally friendly solution with great potential to increase energy efficiency and reduce emissions in industrial processes. Ammonia-water absorption–compression heat pumps (ACHPs) combine the technologies of an absorption and vapor compression heat pump using a zeotropic mixture of ammonia and water as working fluid. The given characteristics, such as the ability to achieve high sink temperatures with comparably large temperature lifts and high coefficient of performance (COP) make the ACHP interesting for utilization in various industrial high temperature applications. This work reviews the state of technology and identifies existing challenges based on conducted experimental investigations. In this context, 23 references with capacities ranging from 1.4 kW to 4500 kW are evaluated, achieving sink outlet temperatures from 45 °C to 115 °C and COPs from 1.4 to 11.3. Existing challenges are identified for the compressor concerning discharge temperature and lubrication, for the absorber and desorber design for operation and liquid–vapor mixing and distribution and the choice of solution pump. Recent developments and promising solutions are then highlighted and presented in a comprehensive overview. Finally, future trends for further studies are discussed. The purpose of this study is to serve as a starting point for further research by connecting theoretical approaches, possible solutions and experimental results as a resource for further developments of ammonia-water ACHP systems at high temperature operation.
Quantitative and Qualitative Image Analysis of In Vitro Co-Culture 3D Tumor Spheroid Model by Employing Image-Processing Techniques
Authors: Sharma Mukta , Goudar Venkanagouda S. , Koduri Manohar Prasad , Tseng Fan Gang , Bhattacharya Mahua
Published: May 2021

This work proposes a novel region-estimation (RE) algorithm using the quantification of colon-cancer (HCT-8) and fibroblasts (NIH3T3) cells to estimate the densest region of colon-cancer cells in in vitro 3D co-cultured spheroids. Cells were labelled with different cell tracker dyes to track the cells. The technique involves staining cells with cell trackers The quantification of HCT-8 and NIH3T3 cells by the RE algorithm leads to distribution pattern analysis of cells from the core to the periphery, which ultimately estimates the densest region of HCT-8 cells in an in vitro 3D cell spheroid. Cell quantification by the RE algorithm was compared with the results of cell quantification by ImageJ software. Results demonstrated the distribution patterns of cells from the core to the peripheral region of the in vitro 3D cell spheroid. The overall experimentation showed that the proposed methodology outperformed state-of-the-art approaches in terms of segmentation, quantification, and reducing biasing error.
CFD Analysis and Taguchi-Based Optimization of the Thermohydraulic Performance of a Solar Air Heater Duct Baffled on a Back Plate
Authors: Thao Pham Ba , Truyen Duong Cong , Phu Nguyen Minh
Published: May 2021

In this paper, a solar air collector duct equipped with baffles on a back plate was numerically investigated. The Reynolds number (Re) was varied from 5000 to 20,000, the angle baffle (a) from 30° to 120°, the baffle spacing ratio (Pr) from 2 to 8, and the baffle blockage ratio (Br) from 0.375 to 0.75 to examine their effects on the Nusselt number (Nu), the friction factor (f), and the thermohydraulic performance parameter (η). The 2D numerical simulation used the standard k-ε turbulence model with enhanced wall treatment. The Taguchi method was used to design the experiment, generating an orthogonal array consisting of four factors each at four levels. The optimization results from the Taguchi method and CFD analysis showed that the optimal geometry of a = 90°, Pr = 6, and Br = 0.375 achieved the maximum η. The influence of Br on all investigated parameters was considerable because as Br increased, a larger primary vortex region was formed downstream of the baffle. At Re = 5000 and the optimal geometry parameters, a maximum η of 1.01 was reached. A baffle angle between 60° and 90° achieved a high Nusselt number due to the impingement heat transfer.
Semantic Matching Based on Semantic Segmentation and Neighborhood Consensus
Authors: Xu Huaiyuan , Chen Xiaodong , Cai Huaiyu , Wang Yi , Liang Haitao , Li Haotian
Published: May 2021

Establishing dense correspondences across semantically similar images is a challenging task, due to the large intra-class variation caused by the unconstrained setting of images, which is prone to cause matching errors. To suppress potential matching ambiguity, NCNet explores the neighborhood consensus pattern in the 4D space of all possible correspondences, which is based on the assumption that the correspondence is continuous in space. We retain the neighborhood consensus constraint, while introducing semantic segmentation information into the features, which makes them more distinguishable and reduces matching ambiguity from a feature perspective. Specifically, we combine the semantic segmentation network to extract semantic features and the 4D convolution to explore 4D-space context consistency. Experiments demonstrate that our algorithm has good semantic matching performances and semantic segmentation information can improve semantic matching accuracy.
MPPT Circuit Using Time Exponential Rate Perturbation and Observation for Enhanced Tracking Efficiency for a Wide Resistance Range of Thermoelectric Generator
Authors: Miao Jie , Chen Houpeng , Lei Yu , Lv Yi , Liu Weili , Song Zhitang
Published: May 2021

The thermoelectric generator (TEG) stands out among many energy harvesters due to its simple structure, small size, rich thermal energy, and the absence of pollution and noise. However, previous studies have rarely probed into the influence of TEG internal resistances on extracting maximum power from TEGs, and the tracking of efficiency is limited. By analyzing the relationship between the tracking efficiency and the TEG internal resistances, a time exponential rate perturbation and observation (P&O) technology is proposed to achieve maximum power point tracking (MPPT) for a wide resistance range of the TEG. Using the time exponential rate P&O, the MPPT circuit observed the power change by comparing the positive-channel metal-oxide semiconductor (PMOS) on-time and perturbs the power by adjusting the negative-channel metal-oxide semiconductor (NMOS) on-time exponentially. The MPPT circuit was implemented in a 110 nm complementary metal-oxide semiconductor (CMOS) process. The tracking efficiency maintained a high level from 98.9 to 99.5%. The applicable range of the TEG resistance was from 1 to 12 Ω, which reflects an enhancement of at least 2.2 times.
Implementation of Portable Automatic Tourniquet with High-Elasticity Biocompatible Strap
Authors: Woo Seong Tak , Park Cheol Woo , Sung Ji Hyun , Park Cheol-Min
Published: May 2021

The most common screening techniques to diagnose a patient’s illness are blood collection and intravenous procedures. To find a collection or injection site, conventional blood collection support tools, such as latex tubing and buckle-type tourniquets, are generally used to compress the patient’s blood vessels. This conventional passive method has significant problems such as latex allergy, vulnerability to site contamination and potential bloodstream infection, and physical damage due to improper use. To overcome the disadvantages of conventional blood collection support tools, an automatic tourniquet with a high-elasticity biocompatible strap was constructed. The automatic tourniquet is designed to automatically adjust the strap length using a motor to control the pressure according to the body dimensions of patients. In order to evaluate the performance of the automatic tourniquet, blood flow characteristics were analyzed using an ultrasound measurement system. The results demonstrated that the automatic tourniquet showed better performance compared with those of other blood collection support tools.
Estimation of the Mechanical Parameters for a Reduced Coupled Flexural–Torsional Beam Model of a Tall Building by a Sub-Structure Approach
Authors: Cluni Federico , Fiorucci Stefano , Gusella Vittorio , Gioffrè Massimiliano
Published: May 2021

The use of equivalent beam models to estimate the dynamical characteristics of complex tall buildings has been investigated by several authors. The main reason is the structural response estimation to stochastic loads, such as wind and earthquake, using a reduced number of degrees of freedom, which reduces the computational costs and therefore gives the designer an effective tool to explore a number of possible structural solutions. In this paper, a novel approach to calibrate the mechanical and dynamical features of a complete 3D Timoshenko beam, i.e., describing bending, shear and torsional behavior, is proposed. This approach is based on explicitly considering the sub-structures of the tall building. In particular, the frames, shear walls and lattice sub-systems are modeled as equivalent beams, constrained by means of rigid diaphragms at different floors. The overall dynamic features of the tall building are obtained by equating the deformation energy of an equivalent sandwich beam with that of the selected sub-structures. Finally, the 3D Timoshenko equivalent beam parameters are calibrated by minimizing a suitable function of modal natural frequencies and static displacements. The closed form modal solution of the equivalent beam model is used to obtain the response to stochastic loads.
Evaluation of Praziquantel Efficacy against Zeuxapta seriolae Infections in Greater Amberjack, Seriola dumerili
Authors: Rigos George , Kogiannou Dimitra , Vasilaki Antigoni , Kotsiri Mado
Published: May 2021

The aim of this study was to evaluate the efficacy of dietary praziquantel (PZQ), a promising fish anthelminthic, against Zeuxapta seriolae, a lethal ectoparasite of greater amberjack (Seriola dumerili Risso) farmed in the Mediterranean and elsewhere. The trial was carried out in small cages (3 m3) in situ, harbouring fish (125 ± 14 g) naturally infected with Z. seriolae, at 25 ± 1 °C water temperature. Praziquantel-medicated diet (150 mg PZQ/kg fish) was delivered for three days against a control group. Measurement of both adults and oncomiracidia of Z. seriolae in sampled gill arches revealed significant differences between the tested groups (PZQ: 19.3 ± 9.8 vs. Control: 3.8 ± 6.3). Considering an estimated efficacy of 80.4% in the medicated fish, the present study indicates that oral PZQ treatments can confront Z. seriolae infections considerably in farmed greater amberjack and, perhaps, replace the commonly used hydrogen peroxidase baths.
WR-SVM Model Based on the Margin Radius Approach for Solving the Minimum Enclosing Ball Problem in Support Vector Machine Classification
Authors: Rizwan Atif , Iqbal Naeem , Ahmad Rashid , Kim Do-Hyeun
Published: May 2021

The generalization error of conventional support vector machine (SVM) depends on the ratio of two factors; radius and margin. The traditional SVM aims to maximize margin but ignore minimization of radius, which decreases the overall performance of the SVM classifier. However, different approaches are developed to achieve a trade-off between the margin and radius. Still, the computational cost of all these approaches is high due to the requirements of matrix transformation. Furthermore, a conventional SVM tries to set the best hyperplane between classes, and due to some robust kernel tricks, an SVM is used in many non-linear and complex problems. The configuration of the best hyperplane between classes is not effective; therefore, it is required to bind a class within its limited area to enhance the performance of the SVM classifier. The area enclosed by a class is called its Minimum Enclosing Ball (MEB), and it is one of the emerging problems of SVM. Therefore, a robust solution is needed to improve the performance of the conventional SVM to overcome the highlighted issues. In this research study, a novel weighted radius SVM (WR-SVM) is proposed to determine the tighter bounds of MEB. The proposed solution uses a weighted mean to find tighter bounds of radius, due to which the size of MEB decreases. Experiments are conducted on nine different benchmark datasets and one synthetic dataset to demonstrate the effectiveness of our proposed model. The experimental results reveal that the proposed WR-SVM significantly performed well compared to the conventional SVM classifier. Furthermore, experimental results are compared with F-SVM and traditional SVM in terms of classification accuracy to demonstrate the significance of the proposed WR-SVM.
An Ensemble One Dimensional Convolutional Neural Network with Bayesian Optimization for Environmental Sound Classification
Authors: Ragab Mohammed Gamal , Abdulkadir Said Jadid , Aziz Norshakirah , Alhussian Hitham , Bala Abubakar , Alqushaibi Alawi
Published: May 2021

With the growth of deep learning in various classification problems, many researchers have used deep learning methods in environmental sound classification tasks. This paper introduces an end-to-end method for environmental sound classification based on a one-dimensional convolution neural network with Bayesian optimization and ensemble learning, which directly learns features representation from the audio signal. Several convolutional layers were used to capture the signal and learn various filters relevant to the classification problem. Our proposed method can deal with any audio signal length, as a sliding window divides the signal into overlapped frames. Bayesian optimization accomplished hyperparameter selection and model evaluation with cross-validation. Multiple models with different settings have been developed based on Bayesian optimization to ensure network convergence in both convex and non-convex optimization. An UrbanSound8K dataset was evaluated for the performance of the proposed end-to-end model. The experimental results achieved a classification accuracy of 94.46%, which is 5% higher than existing end-to-end approaches with fewer trainable parameters. Four measurement indices, namely: sensitivity, specificity, accuracy, precision, recall, F-measure, area under ROC curve, and the area under the precision-recall curve were used to measure the model performance. The proposed approach outperformed state-of-the-art end-to-end approaches that use hand-crafted features as input in selected measurement indices and time complexity.
Towards Improving the Durability and Overall Performance of PV-ETICS by Application of a PCM Layer
Authors: Heim Dariusz , Wieprzkowicz Anna , Knera Dominika , Ilomets Simo , Kalamees Targo , Špitalský Zdenko
Published: May 2021

The main goal of the paper was to numerically analyse the risk of overheating of the Energy Activated External Thermal Insulation Composite System (En-ActivETICS) as an example of Building Integrated Photovoltaics (BIPV). The analyses were conducted with the coupled power flow method (thermal and electrical) for 20 European cities. All locations were analysed considering the local climate in the context of building performance simulation as well as electricity production. The obtained results allowed for the determination of the risk of overheating, which can influence system durability, accelerated thermal ageing, and overall performance. It was revealed that the risk of overheating above 80 °C is possible in almost all locations; however, the intensity considerably differs between southern and northern Europe. The effect of latent heat storage for better thermal stabilization and overall performance was determined numerically for all locations. Finally, the improved solution with a phase change material (PCM) layer beside the PV panel was proposed individually for specific climatic zones, considering the required heat capacity. The maximum panel temperature for improved En-ActivETICS does not exceed 85 °C for any location.
High NO2 Concentrations Measured by Passive Samplers in Czech Cities: Unresolved Aftermath of Dieselgate?
Authors: Vojtisek-Lom Michal , Suta Miroslav , Sikorova Jitka , Sram Radim J.
Published: May 2021

This work examines the effects of two problematic trends in diesel passenger car emissions—increasing NO2/NOx ratio by conversion of NO into NO2 in catalysts and a disparity between the emission limit and the actual emissions in everyday driving—on ambient air quality in Prague. NO2 concentrations were measured by 104 membrane-closed Palmes passive samplers at 65 locations in Prague in March–April and September–October of 2019. NO2 concentrations measured by city stations during those periods were comparable with the average values during 2016–2019. The average measured NO2 concentrations at the selected locations, after correcting for the 18.5% positive bias of samplers co-located with a monitoring station, were 36 µg/m3 (range 16–69 µg/m3, median 35 µg/m3), with the EU annual limit of 40 µg/m3 exceeded at 32% of locations. The NO2 concentrations have correlated well (R2 = 0.76) with the 2019 average daily vehicle counts, corrected for additional emissions due to uphill travel and intersections. In addition to expected “hot-spots” at busy intersections in the city center, new ones were identified, i.e., along a six-lane road V Holešovičkách. Comparison of data from six monitoring stations during 15 March–30 April 2020 travel restrictions with the same period in 2016–2019 revealed an overall reduction of NO2 and even a larger reduction of NO. The spatial analysis of data from passive samplers and time analysis of data during the travel restrictions both demonstrate a consistent positive correlation between traffic intensity and NO2 concentrations along/near the travel path. The slow pace of NO2 reductions in Prague suggests that stricter vehicle NOx emission limits, introduced in the last decade or two, have so far failed to sufficiently reduce the ambient NO2 concentrations, and there is no clear sign of remedy of Dieselgate NOx excess emissions.
Protein–Protein Connections—Oligomer, Amyloid and Protein Complex—By Wide Line 1H NMR
Authors: Bokor Mónika , Tantos Ágnes
Published: May 2021

The amount of bonds between constituting parts of a protein aggregate were determined in wild type (WT) and A53T α-synuclein (αS) oligomers, amyloids and in the complex of thymosin-β4–cytoplasmic domain of stabilin-2 (Tβ4-stabilin CTD). A53T αS aggregates have more extensive βsheet contents reflected by constant regions at low potential barriers in difference (to monomers) melting diagrams (MDs). Energies of the intermolecular interactions and of secondary structures bonds, formed during polymerization, fall into the 5.41 kJ mol−1 ≤ Ea ≤ 5.77 kJ mol−1 range for αS aggregates. Monomers lose more mobile hydration water while forming amyloids than oligomers. Part of the strong mobile hydration water–protein bonds break off and these bonding sites of the protein form intermolecular bonds in the aggregates. The new bonds connect the constituting proteins into aggregates. Amyloid–oligomer difference MD showed an overall more homogeneous solvent accessible surface of A53T αS amyloids. From the comparison of the nominal sum of the MDs of the constituting proteins to the measured MD of the Tβ4-stabilin CTD complex, the number of intermolecular bonds connecting constituent proteins into complex is 20(1) H2O/complex. The energies of these bonds are in the 5.40(3) kJ mol−1 ≤ Ea ≤ 5.70(5) kJ mol−1 range.
A Systematic Review and Meta-Analyses of Interventional Clinical Trial Studies for Gene Therapies for the Inherited Retinal Degenerations (IRDs)
Authors: Tuohy Gearóid P. , Megaw Roly
Published: May 2021

IRDs are one of the leading causes of visual loss in children and young adults. Mutations in over 271 genes lead to retinal dysfunction, degeneration and sight loss. Though no cure exists, gene augmentation therapy has brought hope to the field. This systematic review sought to assess the efficacy of available gene therapy treatments for IRDs. Databases and public resources were searched for randomised controlled trials (RCTs) and non-randomised studies of interventions (NRSIs). Standard methodological procedures were used, including a risk-of-bias assessment. One RCT and five NRSIs were assessed, all for adeno-associated virus two (AAV2)-mediated treatment of RPE-specific 65 kDa (RPE65)-associated LCA (Leber congenital amaurosis). Five outcomes were reported for meta-analyses. Modest improvements in visual acuity, ambulatory navigation/mobility testing or central retinal thickness was observed. There was significant improvement in red and blue light full-field stimulus testing (FST) (red light risk ratio of 1.89, treated v control, p = 0.04; and blue light risk ratio of 2.01, treated v control, p = 0.001). Study design assessment using a ROBIN-I tool (Cochrane Library) showed risk-of-bias judgement to be “low/moderate”, whilst there were “some concerns” for the RCT using a RoB-2 tool (Cochrane Library). Although comparison by meta-analysis is compromised by, amongst other issues, a variable amount of vector delivered in each trial, FST improvements demonstrate a proof-of-principle for treating IRDs with gene therapy.
Treatment of Painful Diabetic Neuropathy—A Narrative Review of Pharmacological and Interventional Approaches
Authors: Gupta Mayank , Knezevic Nebojsa Nick , Abd-Elsayed Alaa , Ray Mahoua , Patel Kiran , Chowdhury Bhavika
Published: May 2021

Painful diabetic neuropathy (PDN) is a common complication of diabetes mellitus that is associated with a significant decline in quality of life. Like other painful neuropathic conditions, PDN is difficult to manage clinically, and a variety of pharmacological and non-pharmacological options are available for this condition. Recommended pharmacotherapies include anticonvulsive agents, antidepressant drugs, and topical capsaicin; and tapentadol, which combines opioid agonism and norepinephrine reuptake inhibition, has also recently been approved for use. Additionally, several neuromodulation therapies have been successfully used for pain relief in PDN, including intrathecal therapy, transcutaneous electrical nerve stimulation (TENS), and spinal cord stimulation (SCS). Recently, 10 kHz SCS has been shown to provide clinically meaningful pain relief for patients refractory to conventional medical management, with a subset of patients demonstrating improvement in neurological function. This literature review is intended to discuss the dosage and prospective data associated with pain management therapies for PDN.
Evaluation of Human Ear Anatomy and Functionality by Axiomatic Design
Authors: Sundar Pratap Sriram , Chowdhury Chandan , Kamarthi Sagar
Published: May 2021

The design of the human ear is one of nature’s engineering marvels. This paper examines the merit of ear design using axiomatic design principles. The ear is the organ of both hearing and balance. A sensitive ear can hear frequencies ranging from 20 Hz to 20,000 Hz. The vestibular apparatus of the inner ear is responsible for the static and dynamic equilibrium of the human body. The ear is divided into the outer ear, middle ear, and inner ear, which play their respective functional roles in transforming sound energy into nerve impulses interpreted in the brain. The human ear has many modules, such as the pinna, auditory canal, eardrum, ossicles, eustachian tube, cochlea, semicircular canals, cochlear nerve, and vestibular nerve. Each of these modules has several subparts. This paper tabulates and maps the functional requirements (FRs) of these modules onto design parameters (DPs) that nature has already chosen. The “independence axiom” of the axiomatic design methodology is applied to analyze couplings and to evaluate if human ear design is a good design (i.e., uncoupled design) or a bad design (i.e., coupled design). The analysis revealed that the human ear is a perfect design because it is an uncoupled structure. It is not only a perfect design but also a low-cost design. The materials that are used to build the ear atom-by-atom are chiefly carbon, hydrogen, oxygen, calcium, and nitrogen. The material cost is very negligible, which amounts to only a few of dollars. After a person has deceased, materials in the human system are upcycled by nature. We consider space requirements, materials cost, and upcyclability as “constraints” in the axiomatic design. In terms of performance, the human ear design is very impressive and serves as an inspiration for designing products in industrial environments.
On the Classification of ECG and EEG Signals with Various Degrees of Dimensionality Reduction
Authors: Fira Monica , Costin Hariton-Nicolae , Goraș Liviu
Published: May 2021

Classification performances for some classes of electrocardiographic (ECG) and electroencephalographic (EEG) signals processed to dimensionality reduction with different degrees are investigated. Results got with various classification methods are given and discussed. So far we investigated three techniques for reducing dimensionality: Laplacian eigenmaps (LE), locality preserving projections (LPP) and compressed sensing (CS). The first two methods are related to manifold learning while the third addresses signal acquisition and reconstruction from random projections under the supposition of signal sparsity. Our aim is to evaluate the benefits and drawbacks of various methods and to find to what extent they can be considered remarkable. The assessment of the effect of dimensionality decrease was made by considering the classification rates for the processed biosignals in the new spaces. Besides, the classification accuracies of the initial input data were evaluated with respect to the corresponding accuracies in the new spaces using different classifiers.
Eating Disorders and Suicidal Behaviors in Adolescents with Major Depression: Insights from the US Hospitals
Authors: Patel Rikinkumar S. , Machado Tanya , Tankersley William E.
Published: May 2021

Objective: To evaluate the odds of association between suicidal ideation and/or attempt with comorbid eating disorders in adolescents with major depressive disorder (MDD). Methods: We conducted a cross-sectional study and included 122,020 adolescents with a primary diagnosis of MDD from the nationwide inpatient sample (NIS, 2012–2014). They were sub-grouped by a comorbid diagnosis of eating disorders (N = 1675). We calculated the adjusted odds ratio (aOR) using a logistic regression model with demographic confounders for associations of eating disorders with suicidal ideation and attempt. Results: Suicidal ideations were seen in a higher proportion of adolescents with eating disorders (46.3% vs. 14.2% in those without eating disorders). On the contrary, a low proportion of adolescents with eating disorders had suicidal attempts (0.9% vs. 39.4% in those without eating disorders). Overall, eating disorders were associated with higher odds for suicidal ideations (aOR 5.36, 95% CI 4.82–5.97) compared to those without eating disorders, but with lower odds of suicidal attempt (aOR 0.02, 95% CI 0.01–0.03). Conclusions: Adolescents with MDD and comorbid eating disorders had five-times increased odds of suicidal ideations but lower odds of a suicide attempt. Self-harm/injurious behaviors are early signs of suicidal ideations in these patients. A collaborative care model is required for the screening, early diagnosis, and management of adolescents with eating disorders to improve their quality of life.
The Acoustics of the Benevento Roman Theatre
Authors: Sukaj Silvana , Ciaburro Giuseppe , Iannace Gino , Lombardi Ilaria , Trematerra Amelia
Published: May 2021

During the Imperial Roman period, thousands of theatres were built. The theatres have three principal elements: the scene building (actor position), the orchestra and the cavea (spectator seating). The theatres were built without a roof, so they were open-air spaces. The theatres were abandoned afterward the barbarian invasions, and during the Middle Ages, homes were built inside the cavea. The theatres were rediscovered during the Renaissance period. Today, ancient theatres are the center of cultural events and are used for various kinds of shows. This work discussed the acoustics of the Roman theatre of Benevento, which was built during the Imperial Age. The theatre was destroyed after the barbaric invasion and it was rebuilt in the first half of the 1900s. The theatre was opened in 1957, and today it is the center of social and cultural activities. Acoustic measurements were carried out according to ISO 3382 standard, placing an omnidirectional sound source on the scene building and in the orchestra, with the measurement microphones along three directions in the cavea. The acoustic characteristics in various seating areas of the cavea were evaluated. Therefore, it possible to understand in which sectors of the theatre the acoustic characteristics are optimal for different types of theatrical performances.
Clearance of Circulating Tumor Cells in Patients with Hepatocellular Carcinoma Undergoing Surgical Resection or Liver Transplantation
Authors: Amado Víctor , González-Rubio Sandra , Zamora Javier , Alejandre Rafael , Espejo-Cruz María Lola , Linares Clara , Sánchez-Frías Marina , García-Jurado Gema , Montero José Luis , Ciria Rubén , Rodríguez-Perálvarez Manuel , Ferrín Gustavo , De la Mata Manuel
Published: May 2021

Background: In patients with hepatocellular carcinoma (HCC), a complete clearance of circulating tumor cells (CTCs) early after liver transplantation (LT) or surgical resection (LR) could prevent tumor recurrence. Methods: prospective pilot study including patients with HCC who underwent LR or LT from September 2017 to May 2020. Enumeration of CTCs was performed in peripheral blood samples (7 mL) using the Isoflux® system (Fluxion Biosciences) immediately before surgery, at post-operative day 5 and at day 30. A clinically relevant number of CTCs was defined as >30 CTCs/sample. Results: 41 HCC patients were included (mean age 58.7 ± 6.3; 82.9% male). LR was performed in 10 patients (24.4%) and 31 patients (75.6%) underwent LT. The main etiology of liver disease was chronic hepatitis C (31.7%). Patients undergoing LR and LT were similar in terms of preoperative CTC count (p = 0.99), but clearance of CTCs within the first month was more pronounced in the LT group. Clusters of CTCs at baseline were associated with incomplete clearance of CTCs at day 30 (54.2% vs. 11.8%, p = 0.005), which in turn impacted negatively on survival (p = 0.038). Conclusion: Incomplete clearance of CTCs after surgery could be a surrogate marker of HCC aggressiveness.
Liver Transplantation for Hepatocellular Carcinoma: A Real-Life Comparison of Milan Criteria and AFP Model
Authors: Brusset Bleuenn , Dumortier Jerome , Cherqui Daniel , Pageaux Georges-Philippe , Boleslawski Emmanuel , Chapron Ludivine , Quesada Jean-Louis , Radenne Sylvie , Samuel Didier , Navarro Francis , Dharancy Sebastien , Decaens Thomas
Published: May 2021

Purpose: To compare the agreement for the criteria on the explant and the results of liver transplantation (LT) before and after adoption of the AFP (α-fetoprotein) model. Methods: 523 patients consecutively listed in five French centers were reviewed to compare results of the Milan criteria period (MilanCP, n = 199) (before 2013) and the AFP score period (AFPscP, n = 324) (after 2013). (NCT03156582). Results: During AFPscP, there was a significantly longer waiting time on the list (12.3 vs. 7.7 months, p < 0.001) and higher rate of bridging therapies (84 vs. 75%, p = 0.012) compared to the MilanCP. Dropout rate was slightly higher in the AFPscP (31 vs. 24%, p = 0.073). No difference was found in the histological AFP score between groups (p = 0.838) with a global agreement in 88% of patients. Post-LT recurrence was 9.2% in MilanCP vs. 13.2% in AFPscP (p = 0.239) and predictive factors were AFP > 2 on the last imaging, downstaging policy and salvage transplantation. Post-LT survival was similar (83 vs. 87% after 2 years, p = 0.100), but after propensity score analysis, the post-listing overall survival (OS) was worse in the AFPscP (HR 1.45, p = 0.045). Conclusions: Agreement for the AFP model on explant analysis (≤2) did not significantly change. AFP score > 2 was the major prognostic factor for recurrence. Graft allocation policy has a major impact on prognosis, with a post-listing OS significantly decreased, probably due to the increase in waiting time, increase in bridging therapies, downstaging policy and salvage transplantation.