bluuebunny/crossref_metadata_2025 huggingface dataset:
Technology Utilization By Midwives In Telemedic Services In Maternal And Child Health Services Field
Authors: SILVIAN NATALIA MEGA
Published: January 2023

Pandemi penyakit covid-19 memberikan dampak dan tekanan yang cukup besar dalam layanan kesehatan diseluruh dunia. Salah satunya adalah gangguan pada layanan kesehatan non-covid. Terjadi penurunan kunjungan baik layanan kesehatan tingkat pertama maupun tingkat ketiga. Data IBI di tahun 2020 menunjukkan terjadi penurunan kunjungan ANC selama pandemi. Dampak lebih luasnya dari penurunan kunjungan maternal ke layanan kesehatan dan penundaan vaksinasi anak antara lain peningkatan morbiditas-mortalitas dan masalah kesehatan baru yang harusnya dapat dicegah. Berdasarkan KMK RI No. HK.01.07/Menkes/413/2020 tentang Pedoman Pencegahan dan Pengendalian Covid-19, pemberian informasi dan segala bentuk pelayanan kesehatan akan dilakukan dengan memanfaatkan teknologi informasi komunikasi atau kita sebut dengan telemedicine. Dengan pemanfaatan teknologi komunikasi, bidan dituntut adanya perubahan model praktik yang menuntut keamanan, terpercaya, namun efektif. Hambatan dalam penggunaan layanan telemedis interaktif antaralain: sebagian masyarakat sebagai bagian dari rutinitas klinis, penyediaan teknologi digital bagi masyarakat terbelakang yang masih dalam pengembangan, pengadaan edukasi sistem digital, kendala internet, perangkat teknologi informasi segi literasi digital dan budaya “lebih senang bertatap muka langsung daripada komunikasi jarak jauh”. Solusi yang ditawarkan yaitu pendidikan kesehatan yang memberikan penjelasan tentang cara pemanfaatan perkembangan teknologi yang telah ada baik media sosial maupun aplikasi dalam meningkatkan layanan kesehatan terhadap ibu dan anak (KIA) di wilayah kerjanya.
DOSIS PUPUK GUANO DAN UREA TERHADAP PERTUMBUHAN DAN HASIL KACANG TUNGGAK (Vigna unguiculata L.)
Authors: Wibowo Sadewo Wahyu , Santosa Sartono Joko , Siswadi Siswadi
Published: January 2023

Penelitian ini berjudul Kajian Dosis Pupuk Guano Dan Urea Terhadap Pertumbuhan Dan Hasil Kacang Tunggak (Vigna unguiculata L.) Penelitian dilaksanakan di Kebun Balai Benih Tanaman Pangan dan Hortikultura Tohudan, Karanganyar mulai Desember 2021 hingga Februari 2022. Metode penelitian menggunakan Rancangan Acak Lengkap (RAL) 2 faktor dan diulang 3 kali. Pupuk guano (G) sebagai faktor 1 tediri dari 4 taraf yaitu: G0 (Kontrol), G1= guano dosis 4,7 gram, G2= guano dosis 9,4 gram, G3= guano dosis 14,1 gram. Pupuk urea (N) sebagai faktor 2 terdiri dari 4 taraf yaitu: N0= (Kontrol), N1= urea dosis 2,7 gram, N2= urea dosis 5,4 gram, N3= urea dosis 8,1 gram. Data dianalisis menggunakan analisis ragam dengan uji lanjut BNJ dengan taraf α = 5%. Hasil penelitian menunjukan bahwa pupuk guano (G) berpengaruh terhadap jumlah daun, luas daun, berat kering brangkasan, berat polong, berat biji dan berat kering biji dengan dosis terbaik 9,4 gram/tanaman. Pupuk urea (N) berpengaruh terhadap tinggi tanaman, jumlah daun, jumlah plong, berat polong dan berat biji dengan dosis terbaik 5,4 gram/tanaman.
KOMBINASI PUPUK KANDANG SAPI DAN PUPUK NPK TERHADAP PERTUMBUHAN DAN HASIL TANAMAN SORGUM
Authors: Eka Kumalasari Novita , Priyono Priyono , Bahri Saiful
Published: January 2023

Penelitian dilakukan untuk mengetahui respon antara pupuk kandang sapi dengan pupuk NPK yang berpengaruh pada pertumbuhan dan hasil tanaman sorgum. Kegiatan dilakukan di kebun percobaan Fakultas Pertanian Universitas Slamet Riyadi Surakarta pada tanggal 18 November 2021 hingga 16 Maret 2022, dengan rancangan acak lengkap (RAL) 2 faktor, kemudian diulang sebanyak 3 kali Faktor pertama menggunakan pupuk kandang sapi (S) dengan 4 taraf yaitu (S0) Control, (S1) dosis 120 gram/polybag, (S2) dosis 240 gram/polybag, (S3) dosis 360 gram/polybag. Faktor kedua pupuk NPK (N), (N0) Control, (N1) dosis 3 gram/polybag, (N2) dosis 6 gram/polybag, (N3) dosis 9 gram/polybag. Kesimpulan penelitian ini adalah perlakuan konsentrasi pupuk  kandang sapi 120 gram/polybag dan dosis pupuk NPK 6 gram/polybag memunculkan hasil terbaik  pada tinggi tanaman 260,07 cm,  jumlah daun  8,51 helai,  lingkar batang 9,43 cm, berat biji per malai 169,67 gram, jumlah biji per malai  1062,65 biji, berat brangkasan basah 1567,23 gram, berat brangkasan kering 1344,66 gram.
APLIKASI IRIGASI SISTEM KAPILER DAN MACAM MEDIA TANAM TERHADAP PERTUMBUHAN DAN HASIL TANAMAN TOMAT (SOLANUM LYCOPERSICUM L.)
Authors: Sajuri Sajuri , Yansyah Yan Andi
Published: January 2023

Penelitian ini bertujuan untuk mengetahui pengaruh aplikasi irigasi sistem kapiler dan berbagai macam media tanam pada tanaman tomat dan interaksinya. Penelitian dilaksanakan di Desa Klidang Wetan Kecamatan Batang Kabupaten Batang Jawa Tengah pada ketinggian 2 m dpl. Rancangan percobaan yang digunakan split plot dengan main plotnya sistem irigasi, sub plotnya yaitu macam media tanam, dengan 3 kali ulangan. Faktor pertama macam irigasi sistem kapiler /W (W1 = irigasi sistem kapiler otomatis, W2 = irigasi sistem kapiler manual) dan faktor kedua adalah macam media tanam /M (M0 = tanah, M1 = tanah + pasir + arang sekam /1:1:1/, M2 = tanah + arang sekam + pupuk kandang /1:1:1, M3 = tanah + pasir + sekam + pupuk kandang /1:1:1). Data dianalisis dengan uji F, jika berbeda nyata dilanjutkan dengan BNT (Beda Nyata Terkecil) 5%. Variabel yang diamati tinggi tanaman, jumlah daun per tanaman, luas daun terluas, diameter batang, panjang akar terpanjang, saat berbunga, jumlah buah per tanaman, bobot buah per buah. bobot buah per tanaman, bobot akar. Hasil penelitian menunjukkan irigasi sistem kapiler berpengaruh nyata terhadap panjang akar terpanjang dan saat berbunga, berbeda nyata pada jumlah daun per tanaman, diameter batang, jumlah buah per tanaman, bobot buah per tanaman. Hasil terbaik dicapai pada aplikasi irigasi sistem kapiler manual. Macam media tanam berpengaruh nyata terhadap variabel yang diamati tinggi tanaman, jumlah daun per tanaman, luas daun terluas, diameter batang, panjang akar terpanjang, saat berbunga, jumlah buah per tanaman, bobot buah per tanaman, variabel pengamatan namun pada variabel bobot buah per buah dan bobot akar berpengaruh tidak nyata. Hasil terbaik dicapai pada perlakuan media tanam  tanah + arang sekam + pupuk kandang (1:1:1). Interaksi antara irigasi sistem kapiler dan macam media tanam berbeda nyata terhadap variabel diameter batang, tinggi tanaman, saat berbunga, dan berbeda tidak nyata terhadap variabel jumlah daun per tanaman, luas daun terluas, panjang akar terpanjang, jumlah buah per tanaman, bobot buah per buah, bobot buah per tanaman, dan bobot akar. Hasil  terbaik dicapai pada aplikasi irigasi sistem kapiler manual dengan macam media tanam  tanah + arang sekam + pupuk kandang (1:1:1).
The Concept Of Muslim Friendly Tourism And Its Implementation In Malaysia
Authors: Ariff Irshad Zahrah Fatimah , Sahari Noorul Huda , Abdul Halim Anis Husna
Published: January 2023

Muslim friendly tourism (MFT) is famous for the services and facilities in compliance with Shariah. Malaysia has developed an impressive tourism but there is lack of awareness among industry players where some of them still do not show interest to implement MFT. The scope of this research covers on the implementation of MFT in Malaysia. The objective is to examine the concept of MFT implemented in Malaysia and its services and facilities provided by the agency. The conceptual study adopts a qualitative method where the data are collected through library research such as from books, articles, journals, reports, and online resources. The collected data were analysed by using content analysis. The finding of this study discover on the implementation of MFT in services and regulation standards. By understanding the actual concept, it can enhance the opportunities of MFT and attract key players to invest.
The Effect of Audit Quality, Financial Condition and Earnings Management on the Going Concern Audit Opinion with Corporate Mechanism as a Moderating Variable
Authors:   Universitas Prima Indonesia, Medan, Indonesia, Hulu Yunus Anugerah , Toni Nagian ,   Universitas Prima Indonesia, Medan, Indonesia, Sitepu Wilsa Road Betterment ,   Universitas Prima Indonesia, Medan, Indonesia
Published: January 2023

Going concern is an accounting term used to describe a business that is expected to operate for the foreseeable future or at least the next 12 months. The concept of going concern is particularly relevant in times of economic difficulties and in some situations auditor may determine that a profitable company may not be a going concern, for example because of significant cash flow difficulties. This research aims to study the influence of audit quality, financial condition and earnings management on the auditor's opinion on going concern with corporate mechanism as a moderating variable. This study analyzes data from the financial statements of 49 manufacturing companies listed on the Indonesian Stock Exchange in 2018-2020. The results of this study provide empirical evidence that giving a going concern audit opinion by the auditor is not based on the quality of the auditor because he acts in accordance with auditing standards. Financial condition and earnings management have a significant effect on the going concern audit opinion. When a company experiences a bad financial condition, it is very likely that it will receive a going concern audit opinion because there are signs of difficulties in maintaining the viability of its business. The corporate mechanism is able to moderate only the influence of financial condition on the going concern audit opinion. Thus, the auditor's opinion on going concern based on analysis of financial reporting data is important for investors and other interested parties who want to be sure of their decisions.
Analytical Support for the Functioning of Collective Investment Funds in Ukraine
Authors:   ", Nashkerska Mariia ,   Lviv Polytechnic",   National University, Lviv, Ukraine, Lytvynenko Nataliia ,   "Lviv Polytechnic" National University, Lviv, Ukraine
Published: January 2023

Collective Investment Funds play an essential role in stimulating the country's economic growth (CIF) and Asset Management Companies (AMC) – financial intermediaries that accumulate investors' funds and professionally invest them in various financial instruments. The article aims to analyze the analytical support, which is the basis for evaluating the work results and risks in the activities of collective investment funds and asset management companies. Relevant information for the analysis of the state and changes in the market of collective investment funds and asset management companies is taken from the Ukrainian Association of Investment Business website (https://www.uaib.com.ua). The article includes a classification of collective investment funds and an analysis of the dynamics of their number, as well as the volume of assets managed by asset management companies. The basic principles of accounting and reporting in collective investment funds were disclosed, and the indicators necessary for risk assessment were characterized. It has been established that the calculation of regulated indicators makes it possible to assess risks only in general. However, to measure and manage risks, it is advisable to develop a scale of dependence on changes in indicators (intensification of negative processes) and the level of risk (minimal, average, significant and critical). Based on the normatively established indicators, it is recommended to improve the method of assessing and measuring the risks of collective investment funds by calculating an integral indicator that would consider each indicator's weighting factor. As part of the risk management system, each level of risk should be offered an appropriate set of tools and measures for minimization.
Specifics of Asset Impairment in the Conditions of Armed Aggression
Authors:   Kyiv National Economic University named after Vadym Hetman, Kyiv, Ukraine, Kulyk Andrii
Published: January 2023

A decrease in the value of the assets of enterprises due to direct damage or destruction, as well as a decrease in expected benefits due to the deterioration of the external environment in which the business operates, are the consequences of the Russian Federation's armed aggression in Ukraine. Therefore, the issue of accounting approaches and procedures for assessing losses from asset impairment becomes relevant. The article aims to define and critically analyze the features of the procedures for the impairment of fixed assets in connection with armed aggression. Signs of impairment are identified, the analysis of which is a prerequisite for assessing losses from the impairment of fixed assets. Cases and problematic aspects of implementing asset impairment procedures in conditions of armed aggression have been identified. Among them are the instability of the external environment, the need to update business plans, the development of several cash flow forecast scenarios, and the need to take into account additional risks from armed aggression in the discount rate. Methods of fair value assessment proposed in international valuation standards were disclosed. When identifying assets subject to an impairment test, it is proposed to create a register of objects that have suffered from armed aggression. When choosing between the assessment of fair value and value-in-use, the author concluded that determining fair value is a more difficult task, as it is based to a greater extent on external market assumptions. In contrast, the calculation of value-in-use is a more feasible task for the company's management, provided that the business is updated on time – plans and forecasts.
Role of antimullarian hormone in the diagnosis of sonographically inconclusive polycystic ovary syndrome
Authors: Banu Hurjahan , Morshed Md Shahed , Tuqan Sadiqa , Akhtar Nazma , Hasanat MA
Published: January 2023

Antimullarian hormone (AMH) is found to be a vital tool for the diagnosis of polycystic ovary syndrome (PCOS). AMH may help in the diagnosis of sonographically inconclusive cases of PCOS. This study measured the AMH level in PCOS to assess its impacts on the diagnosis of the syndrome. This cross-sectional study included 160 newly diagnosed females with PCOS who were diagnosed using a modified revised Rotterdam criteria. Fasting blood was collected to measure AMH by enzyme-linked immunosorbent assay and other hormones [total testosterone, luteinizing hormone and follicle-stimulating hormone] were measured by chemiluminescent microparticle immunoassay. Ovarian USG was done in the follicular phase of the menstrual cycle. Serum AMH≥ 3.5 ng/mL and ovarian volume >10 mL was considered as a combined marker of polycystic ovary (PCO). USG-PCO criteria could detect 84.38% PCO, whereas AMH-PCO criteria 67.5%. There was a lack of agreement between USG-PCO and AMH-PCO criteria [κ=-0.004] in PCOS. AMH-PCO criteria identified 68% of PCO patients undiagnosed by USG-PCO criteria [17/25]. Age [β=-0.172, p=0.040], systolic [β =-0.213, p=0.037] and diastolic blood pressure [β=0.301, p=0.004] had significant predictive associations with AMH by linear regression. AMH had a fair discriminating index for combined-PCO [AUC=0.824] in PCOS patients. In conclusion, AMH assessment can help detect PCOS patients who are inconclusively diagnosed by USG-PCO criteria.
BSMMU J 2022; 15(2): 65-69
Application of toluidine blue stain and neuron specific enolase immunohistochemical stain in the diagnosis of hirschsprung disease
Authors: Islam Tasmia , Banu Sultana Gulshana , Dey Bishnu Pada , Rahman Mohammad Mosiur , Rahman Papiya , Shabnam Ummey Salma , Mahmuda Syeeda Shiraj Um
Published: January 2023

Hirschsprung disease is one of the most common and problematic infancy and childhood maladies. Early and accurate diagnosis is a fundamental step in proper management and prevention of complications. The most reliable method for diagnosis is the histopathological analysis of colorectal biopsies and the typical finding of Hirschsprung disease is the absence of ganglion cells. Toluidine blue stain can act as a double check along with conventional H&E stain for ganglion cell detection. Neuron-specific enolase is an immune-histochemical marker that can also aid in better identifying ganglion cells, especially for small and immature ones. This study aimed to evaluate Toluidine blue stain and Neuron specific enolase immunostain along with conventional H&E stain as a panel for the diagnosis of Hirschsprung disease. This cross-sectional study was conducted from September 2019 to August 2021, involving 55 clinically suspected Hirschsprung disease cases. Paraffin blocks of colorectal biopsy samples were collected from the Department of Pathology, BSMMU. Hematoxylin & Eosin, Toluidine blue stain, and Neuron specific enolase immunohistochemical stain for Hirschsprung disease detection were performed on the sections from the paraffin blocks. Then the sections were examined and an evaluation of the stains was done. Statistical analysis was performed on the tabulated data by chi-square test. Among 55 cases, conventional H&E stain detected ganglion cells in 31 cases, that is 56.4%. Later, Toluidine blue stain and Neuron specific enolase immu- nohistochemical stain detected ganglion cells in 35 cases, that is 63.6%. So, these two addition- al stains were able to detect ganglion cells in four more cases compared to conventional H&E stain. In conclusion, conventional H&E stain, Toluidine blue stain, and NSE immunohisto- chemical stain can improve the diagnostic accuracy of Hirschsprung disease.
BSMMU J 2022; 15(2): 102-106
A 15-year-old boy with an unerupted tooth enclosed by dentigerous cyst associated with odontome
Authors: Shikder Abu HenaMohammod Zakir Hossain , Galib Muhiuddin Mahmud , Siddiqua Fahmida , Jahan Dilara
Published: January 2023

Odontoma are the most common benign odontogenic tumor of epithelial and mesenchymal origin. Usually a conservative approach is the standard treatment protocol for this. Odontoma associated with unerupted tooth andodontogenic cyst aggravates the existing condition. Generally, expansible nature of cystic lesions require surgical excision which is different from isolated non-symptomatic odontomas that can be monitored by periodic radiological examination. There is inadequate evidence on the presence of odontoma-associated cystic lesions in the oral cavity. Therefore, the present case report is aimed at describing the diagnostic clinical, radiological, and histological features together with the surgical management of a dentigerous cyst associated with a compound odontoma. The lesion was surgically removed. No recurrence was observed after 12 months of follow-up.
BSMMU J 2022; 15(3): 201-203
Declining trend of tobacco use in a rural community of Bangladesh: Results of eight years of community interventions
Authors: Zaman M Mostafa , Zaman Ananna , Banik Palash Chandra , Numan Sharker Md Numan , Islam Md Sadekul , Ahmed Jasimuddin , Choudhury Sohel Reza , Choudhury Tanvir Turin
Published: January 2023

Tobacco use in Bangladesh has been high, but there is no report on community-level intervention to reduce tobacco use. The aim of this article is to report the experience of eight years of community intervention to reduce tobacco use in a rural area of Bangladesh. We have done four householdbased surveys (2006, 2008, 2012, and 2014) in Ekhlaspur village of Chandpur district. One man and one woman aged ≥18 years from each of 600 households were selected for each survey randomly to monitor tobacco use in this village concomitant with community interventions. The intervention package included yard meetings, health facility-based counselling, observance of the world no-tobacco days, and periodic lectures in schools. Chi-square for linear trend analysis was done to examine declining trends of prevalence of tobacco use. There were 953 to 1015 participants, approximately half being men in each survey. Their mean age was 44 to 45 years in all surveys. A decline in tobacco use has been observed from 58.8 in 2006 to 43.4% in 2014 (Ptrend=0.000). There has not been any perceivable change in smoking in women because it was either zero or close to zero across surveys. A similar trend was observed in age groups, educational achievements, and hypertension (Ptrend=0.000). Smoking in men declined from 58.3 to 35.2% (Ptrend= 0.000). Community interventions in a sustainable manner can reduce tobacco use in rural communities of Bangladesh.
BSMMU J 2022; 15(3): 157-161
GEOSTRATEGIC ASPECTS OF GLOBAL SECURITY (USING THE EXAMPLE OF RUSSIAN AGGRESSION)
Authors: NIKIFORENKO VOLODYMYR , KURYLIUK YURII , KUKIN IGOR
Published: January 2023

This article examines the issue of violation of the principle of territorial integrity (integrity) or political independence of states from 1946 to 2022. The approach is based on the study and understanding of domestic and foreign literary sources, statistics, legal acts. Russia gained the status of an independent entity in the international arena immediately after the collapse of the Soviet Union. Almost immediately, it began to show signs of aggression. To promote its geopolitical ambitions, it uses gaps in international law, information and psychological measures, corruption, mercenaries, collaborators, blackmail, the law of force, and tries to rewrite history. The course of military conflicts involving Russia in the Republic of Moldova, Chechnya, Georgia, Syria and Ukraine shows that the scale of violations of international treaties, human rights and the rules of warfare has increased in line with the strengthening of its military capabilities. During the hostilities on the territory of independent Ukraine, Russia showed a complete list of signs of aggression, as defined by UN General Assembly Resolution 3314 (XXIX) of December 14, 1974. This shows that before the armed attack on Ukraine, the aggressor was convinced that it could not be brought to justice and punished. It also hoped that his next victim would not be able to receive international political, economic, military and social assistance. Some actions of Russia in the international arena have signs of state terrorism. During the last ten years, a fascist regime has formed in Russia. The abuse of the veto did not lead to the expulsion of the aggressor country from the UN Security Council. To stop Russia’s aggression and prevent similar developments in the future, the issues of improving international law, bringing to justice the political and military leadership of the republic of Belarus and the Russian federation, improving the UN Charter, the powers of the UN General Assembly, depriving Russia of the right to participate in peacekeeping operations.
Kvantifikatori u značenju skupova životinja u hrvatskome i ruskome jeziku
Authors: Milković Lidija
Published: January 2023

U ovome se radu analiziraju i uspoređuju principi jezične kategorizacije kvantifikatora koji označuju skupove životinja u hrvatskome i ruskome jeziku. Budući da u jednojezičnim i dvojezičnim rječnicima hrvatskoga i ruskoga jezika nisu iscrpno zabilježena ograničenja ni kriteriji upotrebe imenica koje označuju životinje uz pojedine kvantifikatore, provedeno je korpusno istraživanje leksičke spojivosti kvantifikatora koje je imalo cilj točnije utvrditi na koji način kvantifikatori klasificiraju skupine životinja u proučavanim jezicima. Ukupno je u radu analizirana upotreba 10 hrvatskih i 10 ruskih kvantifikatora putem alata Sketch Engine u korpusu hrvatskoga jezika hrWaC te korpusu ruskoga jezika ruTenTen11. Uzorak je dobiven ekscerpiranjem jednojezičnih rječnika ruskoga i hrvatskoga jezika. Sličnosti i razlike klasifikacije kategorija životinja i kvantifikatora koji se s njima koriste shematski su prikazani pomoću četiriju tablica koje mogu poslužiti kao pomoć pri prevođenju s jednoga jezika na drugi. Rezultati analize pokazali su da kvantifikatori ne kategoriziraju sve skupine životinja, već one koje su u bliskoj interakciji s ljudima. U radu su korišteni etimološki i povijesni leksikografski izvori kako bi se utvrdilo na koji se način mijenjala leksička spojivost kvantifikatora u značenju skupova životinja te kako bi se odgovorilo na pitanje u kojoj mjeri značenje leksema utječe na principe klasifikacije. Izdvojena su tri modela imenovanja kvantifikatora u značenju skupova životinja, a to su: metonimijski pomak, način kretanja životinja i oblik koji poprima skupina životinja u pokretu. Neki od modela imenovanja ujedno su i klasifikacijski kriteriji po kojima se dijele životinje. Kvantifikatori koji označuju skupove životinja klasificiraju životinjski svijet u oba proučavana jezika prema tome jesu li životinje odrasle ili mlade, po načinu kretanja, pripadnosti pojedinome (nad)razredu životinja, a neki kvantifikatori obuhvaćaju samo jednu vrstu životinja. Kategorije životinjskih vrsta na različit su način obuhvaćene kvantifikatorima u ruskome i hrvatskome jeziku, ali su principi organizacije kategorija veoma slični.
FEATURES OF THE CYTOKINE STATUS OF PATIENTS WITH PSORIASIS TAKING INTO ACCOUNT THE APPLIED TREATMENT METHODS
Authors:   SE «Institute of Dermatology and Venerеology of NAMS of Ukraine», Kutasevych Ya. F. , Dzhoraieva S. K. ,   SE «Institute of Dermatology and Venerеology of NAMS of Ukraine», Kondakova H. K. ,   SE «Institute of Dermatology and Venerеology of NAMS of Ukraine», Mashtakova I. O. ,   SE «Institute of Dermatology and Venerеology of NAMS of Ukraine», Oliinyk O. I. ,   SE «Institute of Dermatology and Venerеology of NAMS of Ukraine», Goncharenko V. V. ,   SE «Institute of Dermatology and Venerеology of NAMS of Ukraine», Shevchenko Z. M. ,   SE «Institute of Dermatology and Venerеology of NAMS of Ukraine»
Published: January 2023

The involvement of complex immune mechanisms in the pathological process in psoriasis actualizes immunological research, since the clinic and course of psoriasis largely depends on the degree of immunological disorders and the imbalance of cytokine regulation of intercellular interactions. The dynamics of these processes during sanogenesis is of some interest. The purpose of the work is to study the cytokine status and its changes in patients with psoriasis depending on the type of treatment. Materials and methods. Cytokine status (IL‑6, IL‑8, IL‑10, IFN-γ and TNF-α levels in blood sera) of 60 patients with psoriasis and 17 practically healthy individuals was determined using immunoenzymatic analysis. Results and discussion. According to the results of the study, a significant imbalance of the indicators of the cytokine status of patients with psoriasis was established, which is difficult to explain by the activation or suppression of any one-cytokine pathway. The obtained data indicate the need to take into account these indicators in the treatment of patients, especially with the use of immunosuppressive agents. Conclusions. When studying the cytokine status of patients with psoriasis before treatment, a significant increase in the production of pro-inflammatory cytokines IL‑6, IL‑8, TNF-α, IFN-γ and the anti-inflammatory cytokine IL‑10 by blood cells was found. The use of therapy methods, which in particular contained immunosuppressive drugs, led to a decrease in pro-inflammatory cytokines and a certain normalization of anti-inflammatory ones. At the same time, a tendency towards a lower effect of GCS on the normalization of cytokine status was noted in comparison with cytostatic drugs in comparison with therapy without immunosuppressive agents.
Multi-Isotope Geochemical Baseline Study of the Carbon Management Canada Research Institutes CCS Field Research Station (Alberta, Canada), Prior to CO
2
Injection
Authors: Utley Rachel E. , Martin-Roberts Emma , Utting Nicholas , Johnson Gareth , Györe Domokos , Zurakowska Marta , Stuart Finlay M. , Boyce Adrian J. , Darrah Thomas H. , Gulliver Pauline , Haszeldine R. Stuart , Lawton Don , Gilfillan Stuart M. V.
Published: January 2023

Carbon capture and storage (CCS) is an industrial scale mitigation strategy for reducing anthropogenic CO
2
from entering the atmosphere. However, for CCS to be routinely deployed, it is critical that the security of the stored CO
2
can be verified and that unplanned migration from a storage site can be identified. A number of geochemical monitoring tools have been developed for this purpose, however, their effectiveness critically depends on robust geochemical baselines being established prior to CO
2
injection. Here we present the first multi-well gas and groundwater characterisation of the geochemical baseline at the Carbon Management Canada Research Institutes Field Research Station. We find that all gases exhibit CO
2
concentrations that are below 1%, implying that bulk gas monitoring may be an effective first step to identify CO
2
migration. However, we also find that predominantly biogenic CH
4
(∼90%–99%) is pervasive in both groundwater and gases within the shallow succession, which contain numerous coal seams. Hence, it is probable that any upwardly migrating CO
2
could be absorbed onto the coal seams, displacing CH
4
. Importantly,
4
He concentrations in all gas samples lie on a mixing line between the atmosphere and the elevated
4
He concentration present in a hydrocarbon well sampled from a reservoir located below the Field Research Station (FRS) implying a diffusive or advective crustal flux of
4
He at the site. In contrast, the measured
4
He concentrations in shallow groundwaters at the site are much lower and may be explained by gas loss from the system or
in situ
production generated by radioactive decay of U and Th within the host rocks. Additionally, the injected CO
2
is low in He, Ne and Ar concentrations, yet enriched in
84
Kr and
132
Xe relative to
36
Ar, highlighting that inherent noble gas isotopic fingerprints could be effective as a distinct geochemical tracer of injected CO
2
at the FRS.
Tying decentralization and income redistribution to fight corruption: Empirical evidence from developed and developing countries
Authors: Bojanic Antonio N.
Published: January 2023

Utilizing a panel data set for OECD and non-OECD countries for the period 1980–2016, I analyze the effects on corruption of interacting different forms of decentralization—fiscal, administrative, political, and overall decentralization—with an indicator of income inequality. The findings demonstrate that fiscal, administrative, and overall decentralization by themselves are not conducive to lowering corruption, but when moderated by the Gini index, corruption levels decrease in all countries. Moreover, as income inequality decreases, the impact of these forms of decentralization in lowering corruption increases, highlighting that decentralization can be an effective tool in combating corruption particularly when income inequality improves. The findings also demonstrate that in non-OECD countries, decentralization is an important tool to fight corruption up to high levels of inequality, proving that decentralization in developing countries is essential even when issues of income distribution have not been fully solved.
Sugammadex shortens the time to extubate and discharge from PACU in patients with tracheobronchial stenosis undergoing rigid bronchoscopy procedures: A retrospective cohort study
Authors: Lu Xiaofei , Li Tingting , Chen Xu , Xu Meiying , Wu Jingxiang , Qiu Yuwei
Published: January 2023

Introduction

Incomplete reversal of neuromuscular blocking drugs can delay patients' rapid recovery and lead to adverse events in the postoperative period, especially in high-risk patients. Sugammadex as a reversal agent, may offer distinct advantages to the scenario where residual neuromuscular blockade may be poorly tolerated. We aimed to investigate the efficacy of sugammadex compared with neostigmine on perioperative outcomes in patients with preoperative tracheal stenosis undergoing rigid bronchoscopy.

Method

We conducted a retrospective cohort study of adults who were diagnosed with primary or secondary tracheobronchial stenosis, and scheduled for interventional therapy under rigid bronchoscopy, at Shanghai Chest Hospital between December 2016 and July 2020. The exposure was categorized into two groups according to the antagonists of muscle relaxants administered after surgery: Group neostigmine vs. Group sugammadex. The primary outcome was the time to extubate after surgery, and the second outcome was the time to discharge from PACU. Perioperative adverse events were recorded.

Results

A total of 98 patients undergoing rigid bronchoscopy procedures were included. Patients in Group sugammadex showed less time to extubate (11 [8, 17] vs. 16 [12, 22] min, P = 0.003) and discharge from PACU (27 [20, 33] vs. 32.5 [24, 44] min, P = 0.013) than in Group neostigmine. The incidence and duration of hypotension during the procedure in Group sugammadex were significantly lower than that in Group neostigmine (18.5 vs. 40.8%, P = 0.038; 0 [0, 0] vs. 0 [0, 8] min, P = 0.036 respectively).

Conclusions

Sugammadex shortens the time to extubate and discharge from PACU in patients with tracheobronchial stenosis, accelerating postoperative recovery.
A refined pH-dependent coarse-grained model for peptide structure prediction in aqueous solution
Authors: Tufféry Pierre , Derreumaux Philippe
Published: January 2023

Introduction: Peptides carry out diverse biological functions and the knowledge of the conformational ensemble of polypeptides in various experimental conditions is important for biological applications. All fast dedicated softwares perform well in aqueous solution at neutral pH.

Methods: In this study, we go one step beyond by combining the Debye-Hückel formalism for charged-charged amino acid interactions and a coarse-grained potential of the amino acids to treat pH and salt variations.

Results: Using the PEP-FOLD framework, we show that our approach performs as well as the machine-leaning AlphaFold2 and TrRosetta methods for 15 well-structured sequences, but shows significant improvement in structure prediction of six poly-charged amino acids and two sequences that have no homologous in the Protein Data Bank, expanding the range of possibilities for the understanding of peptide biological roles and the design of candidate therapeutic peptides.
Optimizing E. coli as a formatotrophic platform for bioproduction via the reductive glycine pathway
Authors: Kim Seohyoung , Giraldo Néstor , Rainaldi Vittorio , Machens Fabian , Collas Florent , Kubis Armin , Kensy Frank , Bar-Even Arren , Lindner Steffen N.
Published: January 2023

Microbial C1 fixation has a vast potential to support a sustainable circular economy. Hence, several biotechnologically important microorganisms have been recently engineered for fixing C1 substrates. However, reports about C1-based bioproduction with these organisms are scarce. Here, we describe the optimization of a previously engineered formatotrophic Escherichia coli strain. Short-term adaptive laboratory evolution enhanced biomass yield and accelerated growth of formatotrophic E. coli to 3.3 g-CDW/mol-formate and 6 h doubling time, respectively. Genome sequence analysis revealed that manipulation of acetate metabolism is the reason for better growth performance, verified by subsequent reverse engineering of the parental E. coli strain. Moreover, the improved strain is capable of growing to an OD600 of 22 in bioreactor fed-batch experiments, highlighting its potential use for industrial bioprocesses. Finally, demonstrating the strain’s potential to support a sustainable, formate-based bioeconomy, lactate production from formate was engineered. The optimized strain generated 1.2 mM lactate —10% of the theoretical maximum— providing the first proof-of-concept application of the reductive glycine pathway for bioproduction.
Design of a biocatalytic cascade for the enzymatic sulfation of unsulfated chondroitin with in situ generation of PAPS
Authors: Monterrey Dianelis T. , Benito-Arenas Raúl , Revuelta Julia , García-Junceda Eduardo
Published: January 2023

Sulfation of molecules in living organisms is a process that plays a key role in their functionality. In mammals, the sulfation of polysaccharides (glycosaminoglycans) that form the proteoglycans present in the extracellular matrix is particularly important. These polysaccharides, through their degree and sulfation pattern, are involved in a variety of biological events as signal modulators in communication processes between the cell and its environment. Because of this great biological importance, there is a growing interest in the development of efficient and sustainable sulfation processes, such as those based on the use of sulfotransferase enzymes. These enzymes have the disadvantage of being 3′-phosphoadenosine 5′-phosphosulfate (PAPS) dependent, which is expensive and difficult to obtain. In the present study, a modular multienzyme system was developed to allow the in situ synthesis of PAPS and its coupling to a chondroitin sulfation system. For this purpose, the bifunctional enzyme PAPS synthase 1 (PAPSS1) from Homo sapiens, which contains the ATP sulfurylase and APS kinase activities in a single protein, and the enzyme chondroitin 4-O-sulfotransferase (C4ST-1) from Rattus norvegicus were overexpressed in E. coli. The product formed after coupling of the PAPS generation system and the chondroitin sulfation module was analyzed by NMR.
Engineering Bacillus subtilis for production of 3-hydroxypropanoic acid
Authors: Garg Abhroop , Jers Carsten , Hwang Hee Jin , Kalantari Aida , Ventina Ildze , Mijakovic Ivan
Published: January 2023

3-Hydroxypropionic acid (3-HP) is a valuable platform chemical that is used as a precursor for several higher value-added chemical products. There is an increased interest in development of cell factories as a means for the synthesis of 3-HP and various other platform chemicals. For more than a decade, concentrated effort has been invested by the scientific community towards developing bio-based approaches for the production of 3-HP using primarily Escherichia coli and Klebsiella pneumoniae as production hosts. These hosts however might not be optimal for applications in e.g., food industry due primarily to endotoxin production and the pathogenic origin of particularly the K. pneumoniae. We have previously demonstrated that the generally recognized as safe organism Bacillus subtilis can be engineered to produce 3-HP using glycerol, an abundant by-product of the biodiesel industry, as substrate. For commercial exploitation, there is a need to substantially increase the titer. In the present study, we optimized the bioprocess conditions and further engineered the B. subtilis 3-HP production strain. Thereby, using glycerol as substrate, we were able to improve 3-HP production in a 1-L bioreactor to a final titer of 22.9 g/L 3-HP.
The trophoblast giant cells of cricetid rodents
Authors: Favaron Phelipe O. , Carter Anthony M.
Published: January 2023

Giant cells are a prominent feature of placentation in cricetid rodents. Once thought to be maternal in origin, they are now known to be trophoblast giant cells (TGCs). The large size of cricetid TGCs and their nuclei reflects a high degree of polyploidy. While some TGCs are found at fixed locations, others migrate throughout the placenta and deep into the uterus where they sometimes survive postpartum. Herein, we review the distribution of TGCs in the placenta of cricetids, including our own data from the New World subfamily Sigmodontinae, and attempt a comparison between the TGCs of cricetid and murid rodents. In both families, parietal TGCs are found in the parietal yolk sac and as a layer between the junctional zone and decidua. In cricetids alone, large numbers of TGCs, likely from the same lineage, accumulate at the edge of the placental disk. Common to murids and cricetids is a haemotrichorial placental barrier where the maternal-facing layer consists of cytotrophoblasts characterized as sinusoidal TGCs. The maternal channels of the labyrinth are supplied by trophoblast-lined canals. Whereas in the mouse these are lined largely by canal TGCs, in cricetids canal TGCs are interspersed with syncytiotrophoblast. Transformation of the uterine spiral arteries occurs in both murids and cricetids and spiral artery TGCs line segments of the arteries that have lost their endothelium and smooth muscle. Since polyploidization of TGCs can amplify selective genomic regions required for specific functions, we argue that the TGCs of cricetids deserve further study and suggest avenues for future research.
Development of risk prediction model for cognitive impairment in patients with coronary heart disease: A study protocol for a prospective, cross-sectional analysis
Authors: Wang Qing , Xu Shihan , Liu Fenglan , Liu Yanfei , Liu Yue , Xu Fengqin
Published: January 2023

Background

Ischemic heart disease and degenerative encephalopathy are two main sources of disease burden for the global elderly population. Coronary heart disease (CHD) and cognitive impairment, as representative diseases, are prevalent and serious illnesses in the elderly. According to recent research, patients with CHD are more likely to experience cognitive impairment and their cognitive ability declines more quickly. Vascular risk factors have been associated with differences in cognitive performance in epidemiological studies, but evidence in patients with CHD is more limited. Inextricably linked between the heart and the brain. Considering the unique characteristics of recurrent cognitive impairment in patients with CHD, we will further study the related risk factors. We tried to investigate the potential predictors of cognitive impairment in patients with CHD through a prospective, cross-sectional study.

Methods

The cross-sectional study design will recruit 378 patients with CHD (≥65 years) from Xiyuan Hospital of China Academy of Chinese Medical Sciences. The subjects' cognitive function is evaluated with MoCA scale, and they are divided into cognitive impairment group and normal cognitive function group according to the score results. Demographic data, disease characteristics (results of coronary CT/ angiography, number of stents implanted, status of diseased vessels), laboratory tests (biochemistry, coagulation, serum iron levels, pulse wave velocity), metabolites (blood samples and intestinal metabolites), and lifestyle (smoking, alcohol consumption, sleep, physical activity) will be assessed as outcome indicators. Compare the two groups and the correlation analysis will be performed on the development of mild cognitive impairment. Mann-Whitney U or X2 test was selected to describe and evaluate the variation, and logistics regression analysis was employed to fit the prediction model. After that, do the calibration curve and decision curve to evaluate the model. The prediction model will be validated by a validation set.

Discussion

To explore the risk factors related to mild cognitive impairment (MCI) in patients with CHD, a new predictive model is established, which can achieve advanced intervention in the occurrence of MCI after CHD. Owing to its cross-sectional study design, the study has some limitations, but it will be further studied by increasing the observation period, adding follow-up data collection or prospective cohort study. The study has been registered with the China Clinical Trials Registry (ChiCTR2200063255) to conduct clinical trials.
Numerical study on landslide dynamic process and its impact damage prediction to brick-concrete buildings, a case from Fenghuang street landslide in Shaanxi, China
Authors: Zhao Zhou , Chen Xing , Wei Jiangbo , Ma Jianquan , Ye Wanjun
Published: January 2023

The study of landslide dynamic process and impact damage has important theoretical and practical significance for landslide risk quantitative assessment. Taking Fenghuang Street landslide in Ningqiang County, Shaanxi Province, China as an example, the dynamic process of landslide and its damage to brick-concrete structure buildings are predicted by using discrete element method. Firstly, a three-dimensional numerical landslide model is established by means of the particle flow code system (PFC3D), which is based on landslide investigation, surveying, engineering exploration and geotechnical testing. Secondly, the whole process of landslide deformation, failure, movement and impact damage was simulated, and the velocity, displacement and impact force of the landslide in the motion process were quantitatively studied. Thirdly, the building model (brick-concrete structure) located at the foot of the landslide was constructed by PFC3D and finite element software (Midas/gen), respectively. The characteristics of deformation and displacement of the buildings after the landslide impact are analyzed, and the impact damage of the landslide is predicted. The results show that the rear edge of Fenghuang Street landslide first deforms and fails, and the leading edge is gradually pushed out. After the locking section of the front edge is broken, the landslide begins to slide as a whole, which is a typical push landslide. The main sliding time of the landslide is about 30 s, the maximum average velocity is 3.2 m/s, and the maximum displacement is about 40 m. After the landslide hits the building, the building is displaced in the moving direction of the landslide, and the wall of the building impacted by the landslide is destroyed, resulting in an collapse evident. The relevant research methodologies and findings in this paper can provide a reference for the risk assessment of the same type of landslides, especially the quantitative assessment of the vulnerability for the brick-concrete buildings at risk.
Adaptive variable-grid least-squares reverse-time migration
Authors: Huang Jianping , Chen Liang , Wang Ziying , Song Cheng , Han Jiale
Published: January 2023

Variable-grid methods have the potential to save computing costs and memory requirements in forward modeling and least-squares reverse-time migration (LSRTM). However, due to the inherent difficulty of automatic grid discretization, conventional variable-grid methods have not been widely used in industrial production. We propose a variable-grid LSRTM (VG-LSRTM) method based on an adaptive sampling strategy to improve computing efficiency and reduce memory requirements. Based on the mapping relation of two coordinate systems, we derive variable-grid acoustic wave equation and its corresponding Born forward modeling equation. On this basis, we develop a complete VG-LSRTM framework. Numerical experiments on a layered model validate the feasibility of the proposed VG-LSRTM algorithm. LSRTM tests on a modified Marmousi model demonstrate that our method can save computational costs and memory requirements with little accuracy loss.
Quantitative evaluation of reservoir quality of tight oil sandstones in chang 7 member of Ordos Basin
Authors: Qiu Xiangliang , Ding Li , Liu Junkai , Yan Zhandong , Bao Yanxin , Tan Chengqian
Published: January 2023

In order to establish a quantitative evaluation system for reservoir quality suitable for tight oil sandstones, in this study, taking the Chang 7 Member in the Maling area of the Ordos Basin as an example, the nuclear magnetic resonance, clay mineral analysis, high pressure mercury injection analysis and logging interpretation technology have been used to carry out a comprehensive evaluation of the pore structures, sand body structures and oil-bearing properties of tight oil sandstone reservoirs. The results show that the pseudo-capillary pressure curves transformed by the NMR T2 spectra are consistent with the capillary pressure curves measured by the core experiments. This method can be used for accurate characterization of the pore structures of the reservoir. The pore structure parameters calculated based on the pseudo-capillary pressure curves can accurately reflect the pore structures of the reservoirs such as micropores-thin throats and complex tortuosity. At the same time, the smoothness feature of conventional logging curves is used to evaluate the sand body structures and heterogeneity of the reservoir, and the apparent energy storage coefficient is introduced to quantitatively evaluate the oil-bearing properties of tight oil reservoirs. The evaluation results are in good agreement with the actual production situation. The larger the apparent energy storage coefficient, the higher the initial output of the oil wells. The evaluation results of the reservoir quality of the tight oil sandstones constructed in this paper are highly consistent with the production status, so the method has broad application prospects.