bluuebunny/crossref_metadata_2025 huggingface dataset:
Tumor-Derived Extracellular Vesicles Activate Normal Human Fibroblasts to a Cancer-Associated Fibroblast-Like Phenotype, Sustaining a Pro-Tumorigenic Microenvironment
Authors: Giusti Ilaria , Di Francesco Marianna , Poppa Giuseppina , Esposito Letizia , D’Ascenzo Sandra , Dolo Vincenza
Published: February 2022

Fibroblasts in the tumor microenvironment have been proven to actively participate in tumor progression; they can be “educated” by cancer cells acquiring an activated state and, as such, are identified as cancer-associated fibroblasts (CAFs); CAFs, in turn, remodel tumor stroma to be more advantageous for cancer progression by modulating several processes, including angiogenesis, immunosuppression, and drug access, presumably driving the chemoresistance. That is why they are believed to hamper the response to clinical therapeutic options. The communication between cancer cells and fibroblasts can be mediated by extracellular vesicles (EVs), composed of both exosomes (EXOs) and microvesicles (MVs). To verify the role of different subpopulations of EVs in this cross-talk, a nearly pure subpopulation of EXO-like EVs and the second one of mixed EXO- and MV-like EVs were isolated from ovarian cancer cells and administered to fibroblasts. It turned out that EVs can activate fibroblasts to a CAF-like state, supporting their proliferation, motility, invasiveness, and enzyme expression; EXO-like EV subpopulation seems to be more efficient in some of those processes, suggesting different roles for different EV subpopulations. Moreover, the secretome of these “activated” fibroblasts, composed of both soluble and EV-associated molecules, was, in turn, able to modulate the response of bystander cells (fibroblasts, tumor, and endothelial cells), supporting the idea that EVs sustain the mutual cross-talk between tumor cells and CAFs.
Ropivacaine Inhibits Lung Cancer Cell Malignancy Through Downregulation of Cellular Signaling Including HIF-1α In Vitro
Authors: Shen Junmei , Han Lina , Xue Yongxian , Li Chao , Jia Huiqun , Zhu Kangsheng
Published: February 2022

Background: Ropivacaine is widely used to induce regional anesthesia during lung cancer surgery. Previous studies reported that amide-linked local anesthetics, e.g., ropivacaine, affected the biological behavior of lung adenocarcinoma cells, but the conclusion is controversial and warrants further study. This study set out to investigate the biological effects of ropivacaine on cultured lung cancer cells and underlying mechanisms.

Methods: Lung cancer cell lines (A549 and H1299) were cultured and then treated with or without ropivacaine (0.5, 1, and 2 mM) for 48 or 72 h. Their proliferation, migration, and invasion together with cell death and molecules including hypoxia inducible factor (HIF)-1α, VEGF, matrix metalloproteinase (MMP)-1, MMP-2, and MMP-9 expression associated with these changes were determined.

Results: Ropivacaine significantly inhibited proliferation and migration, invasion, and cell death in a concentration-dependent manner in both cell lines. Ropivacaine also promoted cell death and induced a concentration- and time-dependent cell arrest towards the G0/G1 phase. Expression of VEGF, MMP-1, MMP-2, MMP-9, and HIF-1α in both cell lines was also inhibited by ropivacaine in a concentration-related manner.

Conclusion: Our data indicated that ropivacaine inhibited lung cancer cell malignancy, which may be associated with downregulation of cell-survival-associated cellular molecules. The translational value of the current work is subjected to further study.
Updated Review on Ozone Therapy in Pain Medicine
Authors: Hidalgo-Tallón Francisco Javier , Torres-Morera Luis Miguel , Baeza-Noci Jose , Carrillo-Izquierdo Maria Dolores , Pinto-Bonilla Rosa
Published: February 2022

The use of medical ozone in the treatment of chronic pain is progressively expanding in Spain and today it is used both in public and private medical centers. However, there is a great lack of knowledge about this technology not only in primary care but also in medical specialties. Although its biochemical bases are well determined and there are various systematic reviews and meta-analyses in the literature that justify its use in pain medicine, some professionals still are prejudiced against it. The evidence level of using medical ozone according SIGN (Scotish Intercollegiate Guideline Network) criteria is similar or superior to most of the techniques used in a Pain Unit. In this paper, we have done a review on ozone therapy in pain medicine, compiling the evidence published about it.
Ozone in Medicine: A Few Points of Reflections
Authors: Re Lamberto
Published: February 2022

Notwithstanding the use of ozone in medicine has become widespread in many countries of the world, its real pharmacological action remains not completely clarified. We know that other than its uses as disinfectant, well documented by the literature since the beginning of the past century, the more recent medical use of ozone in several pathologies as described by the international literature is still poor investigated. Furthermore, following its clinical uses with excellent clinical responses on several heterogeneous diseases and pain, it is now clear that the biological activity of this gas is mediated by graded responses to the mild oxidative stress induced after its application. Thus, the ancestral environment of our cells, whose energy production is strictly bind to oxygen burn, may be mediated by common defenses probably linked to the ubiquitous signaling pathway mediated by Nrf2. Moreover, after the first description of the oxidative stress in the 1970s and the discovery of Nrf2 as transcription factor in 1994, we could observe a rapid growth of the literatures regarding its function as master regulator of a myriad of cellular processes and its association to a multiple pattern of diseases including aging. In conclusion, to our opinion, the Systems Medicine approach could finally give to us the real key to better understand the wide reported efficacy of ozone treatment.
Ectopic Expression of the Allium cepa 1-SST Gene in Cotton Improves Drought Tolerance and Yield Under Drought Stress in the Field
Authors: Liu RuiNa , Jiao TianQi , Zhang ZeXing , Yao Zhang , Li ZhongQing , Wang Saisai , Xin Hongliang , Li YuXia , Wang AiYing , Zhu JianBo
Published: February 2022

In some plants, sucrose: sucrose 1-fructosyltransferase (1-SST) is the first irreversible key enzyme in fructan biosynthesis. Studies have shown that fructan accumulation enhances abiotic stress tolerance of plants. To investigate the role of 1-SST in drought stress responses, a total of 37 cotton plants expressing a 1-SST gene from Allium cepa were developed by Agrobacterium-mediated transformation. Under drought stress in the field, compared with wild-type, ectopic expression of Ac1-SST in cotton resulted in significantly higher soluble sugars (especially 1-kestose), proline and relative water contents, as well as decreased malondialdehyde content, which contributed to maintaining intracellular osmoregulation and reducing membrane damage. In addition, ectopic expression of Ac1-SST in cotton significantly improved the photosynthesis rate, performance of PSII (including Pn, Fv/Fm, WUE, ΦPSII, and PItotal) and plant growth under drought stress. Furthermore, compared with the wild-type, under the droughted field, the yield loss per square meter of transgenic cotton was reduced by an average of 20.9% over two consecutive years. Our results indicate that the Ac1-SST gene can be used to improve drought tolerance and yield of cotton varieties, and might also be a promising drought-resistant gene for improving other crop varieties.
Role of Ethylene Biosynthesis Genes in the Regulation of Salt Stress and Drought Stress Tolerance in Petunia
Authors: Naing Aung Htay , Campol Jova Riza , Kang Hyunhee , Xu Junping , Chung Mi Young , Kim Chang Kil
Published: February 2022

Ethylene plays a critical signaling role in the abiotic stress tolerance mechanism. However, the role of ethylene in regulating abiotic stress tolerance in petunia has not been well-investigated, and the underlying molecular mechanism by which ethylene regulates abiotic stress tolerance is still unknown. Therefore, we examined the involvement of ethylene in salt and drought stress tolerance of petunia using the petunia wild type cv. “Merage Rose” and the ethylene biosynthesis genes (PhACO1 and PhACO3)-edited mutants (phaco1 and phaco3). Here, we discovered that editing PhACO1 and PhACO3 reduced ethylene production in the mutants, and mutants were more sensitive to salt and drought stress than the wild type (WT). This was proven by the better outcomes of plant growth and physiological parameters and ion homeostasis in WT over the mutants. Molecular analysis revealed that the expression levels of the genes associated with antioxidant, proline synthesis, ABA synthesis and signaling, and ethylene signaling differed significantly between the WT and mutants, indicating the role of ethylene in the transcriptional regulation of the genes associated with abiotic stress tolerance. This study highlights the involvement of ethylene in abiotic stress adaptation and provides a physiological and molecular understanding of the role of ethylene in abiotic stress response in petunia. Furthermore, the finding alerts researchers to consider the negative effects of ethylene reduction on abiotic stress tolerance when editing the ethylene biosynthesis genes to improve the postharvest quality of horticultural crops.
The Use of Big Data via 5G to Alleviate Symptoms of Acute Stress Disorder Caused by Quarantine Measures
Authors: Hassani Hossein , Komendantova Nadejda , Unger Stephan , Ghodsi Fatemeh
Published: February 2022

This article investigates the role of Big Data in situations of psychological stress such as during the recent pandemic caused by the COVID-19 health crisis. Quarantine measures, which are necessary to mitigate pandemic risk, are causing severe stress symptoms to the human body including mental health. We highlight the most common impact factors and the uncertainty connected with COVID-19, quarantine measures, and the role of Big Data, namely, how Big Data can help alleviate or mitigate these effects by comparing thestatus quoof current technology capabilities with the potential effects of an increase of digitalization on mental health. We find that, while Big Data helps in the pre-assessment of potentially endangered persons, it also proves to be an efficient tool in alleviating the negative psychological effects of quarantine. We find evidence of the positive effects of Big Data on human health conditions by assessing the effect of internet use on mental health in 173 countries. We found positive effects in 110 countries with 90 significant results. However, increased use of digital media and exclusive exposure to digital connectivity causes negative long-term effects such as a decline in social empathy, which creates a form of psychological isolation, causing symptoms of acute stress disorder.
Hypospadias and Increased Risk for Psychiatric Symptoms in Both Childhood and Adolescence: A Literature Review
Authors: Jin Tingting , Wu Weizhou , Shen Maolei , Feng Haiya , Wang Ya , Liu Shixiong , Li Xin , Zhao Shankun
Published: February 2022

Hypospadias is one of the most common congenital malformations in boys. Due to abnormal appearance in the penis with abnormal urination and erection, patients with hypospadias were vulnerable to suffering from stress and psychiatric difficulties. The present study aims to summarize all the current evidence of the association between hypospadias and the risk of psychiatric disorders by a comprehensive review. Seventeen clinical studies were identified in the four electronic databases. A total of 953,872 participants were involved, while 15,729 of them were hypospadiac patients and the remaining 938,143 were normal controls. The standard age for surgery for hypospadias ranged from 20.4 months to 21.5 years. Eight out of seventeen (8/17, 47%) included studies explicitly showed that patients with hypospadias had a significantly higher risk of psychosocial disorders (all P < 0.05). Specific types of psychiatric disorders included depression, anxiety, shyness, timidness, isolation, fear of ridicule, attention-deficit hyperactivity, autism spectrum, behavioral/emotional disorders, temper tantrums, emotionality, affective, psychosexual problems, and suicidal tendencies. Based on this review, psychiatric illnesses are frequently detected in hypospadiac patients' childhood, thus proper psychiatric guidance and early interventions from physicians, nurses, and parents may help these children to grow into less affected men.
Parental Reflective Functioning as a Moderator of the Link Between Prematurity and Parental Stress
Authors: Dollberg Daphna G. , Harlev Yael , Malishkevitch Sivan , Leitner Yael
Published: February 2022

We examined group differences between parents, both mothers and fathers, of premature and full-term infants to determine whether they differed in their reports of subjective parenting stress and in their level of parental reflective functioning (PRF). We also tested whether each parent's reflective functioning moderated the links between birth status (prematurity vs. full-term) and parenting stress. A sample of 73 cohabiting, heterosexual Israeli families with a premature (28–36th week gestational age, N = 34) or full-term infant (37th week and above gestational age, N = 39) participated, comprising the two parents' groups. Infants' age averaged 7.07 months (SD = 1.28). Each parent completed the Parent Stress Inventory (PSI) individually to determine his/her subjective personal and childrearing stress levels. The Parent Development Interview (PDI-R2-S) was used to obtain each parent's PRF (self and child/relation-focused) level. Findings showed that the premature and full-term parents did not differ in their PSI scores or PRF levels. However, mothers' self-focused PRF moderated the link between prematurity and personal parenting stress, whereas fathers' self-focused PRF moderated the link between prematurity and childrearing parenting stress. Furthermore, fathers' and mothers' PRF operated differently in the premature and full-term parents' groups. The findings highlight the importance of mothers' and fathers' PRF in predicting parents' subjective stress in general and particularly in the case of infant prematurity. We discuss these findings and their relevance for preventive and therapeutic perinatal interventions.
Rethinking COVID-19 and Beyond: Prevention, Remedies, and Recovery
Authors: Maffetone Philip B. , Laursen Paul B.
Published: February 2022

In a relatively short timeframe, millions of deaths and illnesses associated with COVID-19 have been reported, accompanied by substantial economic losses, and overall, negatively impacting society. This experience should serve as a wakeup call to those in public health and healthcare, along with politicians and citizens: COVID-19 is considered a predictable and preventable disaster. While various reactive responses to address the pandemic were implemented, some with adverse effects, proactive measures in the years before COVID-19 were neglected. Predominately this involved the development of a preventable overfat pandemic, which played a key role in both rising rates of chronic disease, the comorbidities that increase the risk for COVID-19, along with associated inflammation and malnutrition. This increased the risk of infection in billions of people worldwide, which, in essence, primed society for high rates of COVID-19 infection. Excess body fat evolves primarily from poor nutrition, particularly the overconsumption of sugar and other refined carbohydrates, which replace the vital nutrients needed for optimal immune function. Sugar and refined carbohydrates must be considered the new tobacco, as these foods are also devoid of nutrients, and underly inflammatory chronic diseases. A balanced diet of nutrient-dense wholefood must be emphasized to combat infectious and inflammatory diseases. Implementing proactive preventive lifestyle changes must begin now, starting with simple, safe, and inexpensive dietary modifications that can quickly lead to a healthier population.
Is Cold Apparent Temperature Associated With the Hospitalizations for Osteoporotic Fractures in the Central Areas of Wuhan? A Time-Series Study
Authors: Zhang Faxue , Zhang Xupeng , Zhou Guangwen , Zhao Gaichan , Zhu Shijie , Zhang Xiaowei , Xiang Nan , Zhu Wei
Published: February 2022

Osteoporosis is alarming problem due to aggravation of global aging, especially in China. Osteoporotic fracture (OF) is one of the most severe consequents of osteoporosis. Many previous studies found that environmental factors had adverse effects on human health. Cold temperature was associated with OF and bone metabolism in prior observational and experimental researches. However, few studies had been conducted on the acute effect of low temperature and OF. Data on daily meteorological factors and hospitalizations for OF were collected from Wuhan, China, between January 1, 2017 to December 24, 2019. Apparent temperature (AT), comprehensively considered a variety of environmental factors, was calculated by ambient temperature, relative humidity and wind speed. A generalized linear regression model combined with distributed lag non-linear regression model (DLNM) with quasi-Poisson link was used to explore the association between AT and the number of hospitalizations for OF. Subgroup analyses stratified by gender, age and the history of fracture were applied for detecting susceptible people. The exposure-response curve of AT and OF were generally U-shaped with lowest point at 25.8°C. The significant relationship of AT-OF existed only in cold effect (−2.0 vs. 25.8°C) while not in warm effect (37.0 vs. 25.8°C). Statistically significant risks of OF for cold effects were only found in females [RR = 1.12 (95%CI: 1.02, 1.24) at lag 2 day], aged <75 years old [RR = 1.18 (95%CI: 1.04, 1.33) and 1.17 (95%CI: 1.04, 1.33) at lag 2 and 3 days, respectively] and people with history of fracture [RR = 1.39 (95%CI: 1.02, 1.90) and 1.27 (95%CI: 1.05, 1.53) at lag 1 and 2 days, respectively]. The significant associations of AT on OF were only found in cold effect. The females, people aged <75 years and people with history of fracture possibly appeared to be more vulnerable. Public health departments should pay attention to the negative effect of cold AT and take measures in time.
Survey on Deep Learning-Based Point Cloud Compression
Authors: Quach Maurice , Pang Jiahao , Tian Dong , Valenzise Giuseppe , Dufaux Frederic
Published: February 2022

Point clouds are becoming essential in key applications with advances in capture technologies leading to large volumes of data. Compression is thus essential for storage and transmission. In this work, the state of the art for geometry and attribute compression methods with a focus on deep learning based approaches is reviewed. The challenges faced when compressing geometry and attributes are considered, with an analysis of the current approaches to address them, their limitations and the relations between deep learning and traditional ones. Current open questions in point cloud compression, existing solutions and perspectives are identified and discussed. Finally, the link between existing point cloud compression research and research problems to relevant areas of adjacent fields, such as rendering in computer graphics, mesh compression and point cloud quality assessment, is highlighted.
A Study on Second Harmonic Excitation in Electron Beam–Plasma Instability
Authors: Rafiei Maryamsadat , Sahrai Mostafa , Hosseinpour Mahboub , Esfandyari-Kalejahi Abdolrasoul
Published: February 2022

The electron beam–plasma interaction is a fundamental nonlinear plasma process that is frequently taking place in space and laboratory plasmas. Such an interaction is found to generate electromagnetic waves observed in space plasmas. Using the two-dimensional electromagnetic particle in cell simulations, we investigated the second harmonic excitation in the electron beam–plasma instability. Results showed that, first, the Langmuir waves are excited in the system; then at longer time scales, following the excitation of ion acoustic waves, the second harmonic electromagnetic waves are excited. We found that in the case of background pair plasmas, where the masses are the same, there is no signature of waves in the second harmonic, which is a direct verification of the three-wave coupling mechanism for the generation of electromagnetic waves in the second harmonic.
Engineering A Low-Cost Kelp Aquaculture System for Community-Scale Seaweed Farming at Nearshore Exposed Sites via User-Focused Design Process
Authors: St-Gelais Adam T. , Fredriksson David W. , Dewhurst Tobias , Miller-Hope Zachary S. , Costa-Pierce Barry Antonio , Johndrow Kathryn
Published: February 2022

For over 50 years, government fishery agencies have recognized the need to transition excess fishing capacity in coastal waters to aquaculture. For the most part, investment strategies to move wild capture and harvest efforts into aquaculture have failed since the technology and capital expense for entry, such as large fish pens, was not conducive for acceptance. In contrast, low trophic level aquaculture of shellfish and seaweeds is suitable as an addition to the livelihoods of seasonal fishing communities and to those displaced by fishery closures, especially if vessels and gear can be designed around existing fishing infrastructures, thus allowing fishers to maintain engagement with their primary fishery, while augmenting income via aquaculture. In this study, an inexpensive, lightweight, and highly mobile gear for kelp seaweed farming was developed and tested over a 3-year period in southern Maine, USA. The system was different from existing kelp farming operations used in nearshore waters that use low-scope mooring lines, and heavy, deadweight anchors. Instead, a highly mobile, easy to deploy system using lightweight gear was designed for exposed conditions. The entire system fit into fish tote boxes and was loadable onto a standard pickup truck. The seaweed system had small but efficient horizontal drag embedment anchors connected to a chain catenary and pretensioned with simple subsurface flotation. The system was able to be deployed and removed in less than 4 h by a crew of three using a 10 m vessel and produced a harvest of 12.7 kg/m over an 8-month fall-winter growth period. The target group for this seaweed research and development effort were coastal fishing communities who move seasonally into non-fishing occupations in service industries, such as construction, retail, etc. An economic assessment suggests farmers would realize an 8% return on investment after3 years and $13.50/h greater income as compared to a non-farming off season job at minimum wage. This low-cost seaweed farming system for fall-winter operations fits well into a “livelihood” strategy for fishing families who must work multiple jobs in the offseason when their main fishery is unavailable.
Dapagliflozin-Associated Euglycemic Diabetic Ketoacidosis in a Patient Who Underwent Surgery for Pancreatic Carcinoma: A Case Report
Authors: Luo Xiaoqian , Ji Ran , Lu Weina , Zhu Hong , Li Libin , Hu Jun
Published: February 2022

Diabetic ketoacidosis (DKA), an acute and life-threatening complication of diabetes, is a metabolic disorder caused by insulin deficiency and an increase in counter-regulatory hormones. Several cases of DKA without marked hyperglycemia have been reported and are defined as euglycemic DKA (eu-DKA). The use of sodium-glucose cotransporter 2 inhibitors (SGLT2is) is associated with the occurrence of eu-DKA, of which, dapagliflozin is one of the agents. In this study, we report a case of dapagliflozin-associated eu-DKA following surgery for pancreatic carcinoma. A 57-year-old woman presented with acute abdominal pain after surgery for pancreatic carcinoma. Emergency exploratory laparotomy was performed because of suspicion of gastrointestinal perforation based on a CT scan. The surgeons observed that the stomach was significantly dilated but not perforated. Meanwhile, the patient developed shock and severe acidosis. A further examination confirmed the diagnosis of dapagliflozin-associated eu-DKA. We reviewed the precipitating factors and mechanisms of SGLT2i-associated eu-DKA and discussed the treatment and prevention of this condition. Clinicians need to be alert of the occurrence of SGLT2i-associated eu-DKA in patients treated with this drug in the perioperative period.
Cytosolic Nipah Virus Inclusion Bodies Recruit Proteins Without Using Canonical Aggresome Pathways
Authors: Becker Nico , Heiner Anja , Maisner Andrea
Published: February 2022

Nipah virus (NiV) is a BSL-4 classified zoonotic paramyxovirus that causes respiratory or encephalitic diseases. A hallmark of NiV infections, as with all cell infections caused by non-segmented negative-strand RNA viruses, is the formation of cytoplasmic inclusion bodies (IBs). We previously showed that cytosolic NiV IBs, which are formed in infected cells or in cells minimally expressing the NiV nucleocapsid proteins, are associated with the microtubule-organizing center (MTOC) marker γ-tubulin. They also recruit overexpressed cytosolic proteins that are not functionally required for viral replication in IBs and that otherwise might form toxic protein aggregates. Therefore, NiV IBs are thought to share some functional properties with cellular aggresomes. The fact that aggresomes were not found in NiV-infected cells supports the idea that NiV IBs are successfully reducing the proteotoxic stress in infected cells. Only if the proteasome-ubiquitin system is artificially blocked by inhibitors, cellular aggresomes are formed in addition to IBs, but without colocalizing. Although both structures were positive for the classical aggresome markers histone deacetylase 6 (HDAC6) and Bcl-2-associated athanogene 3 (BAG3), they clearly differed in their cellular protein compositions and recruited overexpressed proteins to different extents. The further finding that inhibition of aggresome pathways by HDAC6 or microtubule (MT) inhibitors did neither interfere with IB formation nor with protein sequestration, strengthens the idea that cytosolic NiV IBs can assume some aggresome-like functions without involving active transport processes and canonical cellular aggresome pathways.
A Psychological Evaluation Model of a Good Conversation in Knowledge Creative Activities by Multiple People
Authors: Tsujimura Sohei , Yairi Motoki , Okita Takayoshi
Published: February 2022

Japanese companies have been focusing on enhancing the knowledge creative activities of older office workers in recent years. In addition, the way of working in the office has been shifting from traditional divisional routine work to collaborative or creative work, and office spaces are becoming quieter, with an increasing number of extremely quiet spaces (noise level < 40 dB). A sound environment that is too quiet gives workers the impression that it is difficult to converse with others, because they are worried about what people around them may think. The appearance of the knowledge creative society in recent years has led to a desire for changes in the workplace environment to improve the productivity of intellectual activities. To realize a sound environment that encourages knowledge creative activities, study outcomes need to be accumulated. Therefore, to clarify what kind of sound environment would be appropriate for knowledge creative activities by multiple people, we conducted psychoacoustic experiments to examine the effects of sound pressure level (signal-to-noise ratio), type of sound, and reverberation time in conference rooms on the impression of a “good conversation”. In addition, we considered a causal model for the psychological evaluation of a “good conversation” by conducting a multiple regression analysis of psychological evaluations of the experimental participants. The results indicated that a sound environment considered too quiet for multiple people to have discussions about knowledge creative activities lowers the impression of a “good conversation”, whereas high levels of relaxation lead to the impression of a “good conversation”.
Data Extraction Method for Industrial Data Matrix Codes Based on Local Adjacent Modules Structure
Authors: Liao Licheng , Li Jianmei , Lu Changhou
Published: February 2022

A 2D barcode is a reliable way to provide lifetime traceability of parts that are exposed to harsh environments. However, there are considerable challenges in adopting mobile cameras to read symbols directly marked on metal surfaces. Images captured by mobile cameras are usually of low quality with poor contrast due to the reflective surface of 2D barcode symbols. To deal with this problem, a novel laser-marked Data Matrix symbols reading method based on deep learning is proposed for mobile phone captured images. Utilizing the barcode module features, we train different convolutional neural network (CNN) models to learn the colors of two adjacent modules of a Data Matrix symbol. Depending on whether the colors of the two adjacent modules are the same or not, an edge image is transformed from a square grid, which is the same size as the barcode. A correction method based on the KM algorithm is used to get a corrected edge image, which helps to reconstruct the final barcode image. Experiments are carried out on our database, and the results show that the proposed algorithm outperforms in high accuracy of barcode recognition.
Comprehensive Review of the Components in Cat’s Claw (Uncaria tomentosa) and Their Antibacterial Activity
Authors: Blanck Jason J. , Huebner Thomas M. , Rolls Alyssa M. , Cornell Josh S. , Hwang Candy S.
Published: February 2022

Cat’s claw (Uncaria tomentosa (Willd. ex Schults) DC.), a plant that is exceptionally rich in phytochemicals, has been used for centuries by the indigenous people of South and Central America as a therapeutic and is currently widely exported for medicinal purposes. Extracts and individual components have shown considerable potential as antibacterials in the literature. The purpose of this review is twofold: first, to provide a substantiated, comprehensive collection of the known chemical constituents of U. tomentosa, including their detailed structures; second, to identify those components that offer some promise as antibacterials based on the research to date. Bacterial resistance to currently available antibiotics continues to increase and is widely recognized as an impending, potentially catastrophic, problem. There is research to suggest that U. tomentosa components may have antibacterial potential individually or synergistically with established antibiotics against microbes, including Borrelia burgdorferi, the causative agent of Lyme disease. It is our intention that this review will provide a valuable resource to investigators in search of new antimicrobials to meet the daunting challenge of antibiotic resistance.
A Novel Method to Determine the Optimal Location for a Cellular Tower by Using LiDAR Data
Authors: Bharadwaj Shruti , Dubey Rakesh , Zafar Md Iltaf , Tiwary Saurabh Kr , Faridi Rashid Aziz , Biswas Susham
Published: February 2022

The cellular industry faces challenges in controlling the quality of signals for all users, given its meteoric growth in the last few years. The service providers are required to place cellular towers at the optimal location for providing a strong cellular network in a particular region. However, due to buildings, roads, open spaces, etc., of varying topography in 3D (obstructing the signals) and varying densities of settlements, finding the optimal location for the tower becomes challenging. Further, in a bigger area, it is required to determine the optimum number and locations for setting up cellular towers to ensure improved quality. The determination of optimum solutions requires a signal strength prediction model that needs to integrate terrain data, information of cellular tower with users’ locations, along with tower signal strengths for predictions. Existing modeling practices face limitations in terms of the usage of 2D data, rough terrain inputs, and the inability to provide detailed shapefiles to GIS. The estimation of optimum distribution of cellular towers necessitates the determination of a model for the prediction of signal strength at users’ locations accurately. Better modeling is only possible with detailed and precise data in 3D. Considering the above needs, a LIDAR data-based cellular tower distribution modeling is attempted in this article. The locations chosen for this research are RGIPT, UP (45 Acre), and Shahganj, Agra, UP, India (6 km2). LiDAR data and google images for the project sites were classified as buildings and features. The edges of overground objects were extracted and used to determine the routes for transmission of a signal from the tower to user locations. The terrain parameters and transmission losses for every route are determined to model the signal strength for a user’s location. The ground strength of signals is measured over 1000 points in 3D at project sites to compare with modeled signal strengths (an RMSE error 3.45). The accurate model is then used to determine the optimum number and locations of cellular towers for each site. Modeled optimum solutions are compared with existing tower locations to estimate % over design or under design and the scope of improvement (80% users below −80 dB m improves to 70% users above −75 dB m).
(ω,c)-Periodic Solutions to Fractional Differential Equations with Impulses
Authors: Ren Lulu , Wang JinRong
Published: February 2022

This paper deals with the (ω,c)-periodic solutions to impulsive fractional differential equations with Caputo fractional derivative with a fixed lower limit. Firstly, a necessary and sufficient condition of the existence of (ω,c)-periodic solutions to linear problem is given. Secondly, the existence and uniqueness of (ω,c)-periodic solutions to semilinear problem are proven. Lastly, two examples are given to demonstrate our results.
On Λ-Fractional Analysis and Mechanics
Authors: Lazopoulos Konstantinos
Published: February 2022

Λ-Fractional analysis was introduced to fill up the mathematical gap exhibited in fractional calculus, where the various fractional derivatives fail to fulfill the prerequisites demanded by differential topology. Nevertheless, the various advantages exhibited by the fractional derivatives, and especially their non-local character, attracted the interest of physicists, although the majority of them try to avoid it. The introduced Λ-fractional analysis can generate fractional geometry since the Λ-fractional derivatives generate differentials. The Λ-fractional analysis is introduced to mechanics to formulate non-local response problems with the demanded mathematical accuracy. Further, fractional peridynamic problems with horizon are suggested.
Effect of Extraction Methodology on the Phytochemical Composition for Camelia sinensis “Powdered Tea Extracts” from Different Provenances
Authors: Barreira Sérgio , Silva André M. N. , Moutinho Carla , Seo Ean-Jeong , Hegazy Mohamed-Elamir F. , Efferth Thomas , Gomes Lígia Rebelo
Published: February 2022

(1) Background: beverages based on extracts from Camellia sinensis are popular worldwide. Due to an increasing number of processed teas on the market, there is a need to develop unified classification standards based on chemical analysis. Meanwhile, phytochemical characterizations are mainly performed on tea samples from China (~80%). Hence, data on teas of other provenances is recommended. (2) Methods: in the present investigation, we characterized lyophilised extracts obtained by infusion, maceration and methanolic extraction derived from tea samples from China, Japan, Sri Lanka and Portugal by phytochemistry (catechins, oxyaromatic acids, flavonols, alkaloids and theanine). The real benefits of drinking the tea were analysed based on the bioavailability of the determined phytochemicals. (3) Results: the infusions revealed the highest total phenolic contents (TPC) amounts, while methanolic extracts yielded the lowest. The correlation matrix indicated that the levels of phenolic compounds were similar in the infusions and methanolic samples, while extractions made by maceration were significantly different. The differences could be partially explained by the different amounts of (-)-epigallocatechin gallate (EGCG), (-)-epicatechin gallate (ECG) and gallic acids (GA). The catechin percentages were significantly lower in the macerations, especially the quantity of EGCG decreases by 4- to 5-fold after this process. (4) Conclusions: the results highlight the importance of the processing methodology to obtain “instant tea”; the composition of the extracts obtained with the same methodology is not significantly affected by the provenance of the tea. However, attention should be drawn to the specificities of the Japanese samples (the tea analysed in the present work was of Sencha quality). In contrast, the extraction methodology significantly affects the phytochemical composition, especially concerning the content of polyphenols. As such, our results indicate that instant tea classification based on chemical composition is sensible, but there is a need for a standard extraction methodology, namely concerning the temperature and time of contact of the tea leaves with the extraction solvent.
Impacts of Resveratrol and Pyrogallol on Physicochemical, Mechanical and Biological Properties of Epoxy-Resin Sealers
Authors: Kharouf Naji , Sauro Salvatore , Hardan Louis , Fawzi Amr , Suhanda Ilona Eveline , Zghal Jihed , Addiego Frédéric , Affolter-Zbaraszczuk Christine , Arntz Youri , Ball Vincent , Meyer Florent , Haikel Youssef , Mancino Davide
Published: February 2022

This study aimed at evaluating the physicochemical and biological properties of experimental epoxy-resin sealers containing polyphenols such as resveratrol and pyrogallol. A conventional epoxy resin (OB) was modified by adding different concentrations of resveratrol (RS) or pyrogallol (PY) to its composition. Antibacterial and antioxidant activities, mechanical properties, along with wettability and morphological changes were investigated. The results were statistically analyzed using ANOVA and multiple comparison tests (α = 0.05). The incorporation of the tested polyphenols into the epoxy resin enhanced its mechanical properties. PY demonstrated much better antioxidant and antibacterial activities than RS, which were associated with a higher release of PY. In contrast, PY showed a higher cytotoxicity than OB and OB doped with RS. OB containing PY presented a rougher surface and higher water absorption than OB doped with RS. Both tested polyphenols caused no notable changes to the overall porosity of OB. Resveratrol and pyrogallol may not only influence the morphology and mechanical properties of epoxy-resin sealers, but could also enhance antioxidant activity and antibacterial effects against Enterococcus faecalis. Most epoxy-resin sealers currently available in the market can be considered as “passive” materials. Thus, doping their composition with specific polyphenols may be a suitable strategy to confer some antibacterial properties, antioxidant potential, along with improvement of some mechanical properties.
Selenium Nanoparticles with Prodigiosin Rescue Hippocampal Damage Associated with Epileptic Seizures Induced by Pentylenetetrazole in Rats
Authors: Al Omairi Naif E. , Albrakati Ashraf , Alsharif Khalaf F. , Almalki Abdulraheem S. , Alsanie Walaa , Abd Elmageed Zakaria Y. , Zaafar Dalia , Lokman Maha S. , Bauomy Amira A. , Belal Saied K. , Abdel-Daim Mohamed M. , Abdel Moneim Ahmed E. , Alyami Hussain , Kassab Rami B.
Published: February 2022

Background: Prodigiosin (PDG) is a red pigment synthesized by bacterial species with important pharmaceutical and biological activities. Here, we investigated the neuroprotective and anticonvulsant activities of green biosynthesized selenium formulations with PDG (SeNPs-PDG) versus pentylenetetrazole (PTZ)-induced epileptic seizures. Methods: Rats were assigned into six experimental groups: control; PTZ (60 mg/kg, epileptic model); sodium valproate (200 mg/kg) + PTZ; PDG (300 mg/kg) + PTZ; sodium selenite (0.5 mg/kg) + PTZ; and SeNPs-PDG (0.5 mg/kg) + PTZ. The treatment duration is extended to 28 days. Results: SeNPs-PDG pre-treatment delayed seizures onset and reduced duration upon PTZ injection. Additionally, SeNPs-PDG enhanced the antioxidant capacity of hippocampal tissue by activating the expression of nuclear factor erythroid 2–related factor 2 and innate antioxidants (glutathione and glutathione derivatives, in addition to superoxide dismutase and catalase) and decreasing the levels of pro-oxidants (lipoperoxidation products and nitric oxide). SeNPs-PDG administration inhibited inflammatory reactions associated with epileptic seizure development by suppressing the production and activity of glial fibrillary acidic protein and pro-inflammatory mediators, including interleukin-1 beta, tumor necrosis factor-alpha, cyclooxygenase-2, inducible nitric oxide synthase, and nuclear factor kappa B. Moreover, SeNPs-PDG protected against hippocampal cell loss following PTZ injection by decreasing the levels of cytosolic cytochrome c, Bax, and caspase-3 and enhancing the expression of anti-apoptotic Bcl-2. Interestingly, SeNPs-PDG restored the PTZ-induced imbalance between excitatory and inhibitory amino acids and improved monoaminergic and cholinergic transmission. Conclusions: These promising antioxidative, anti-inflammatory, anti-apoptotic, and neuromodulatory activities indicate that SeNPs-PDG might serve as a naturally derived anticonvulsant agent.
Characterisation of Severe Traumatic Brain Injury Severity from Fresh Cerebral Biopsy of Living Patients: An Immunohistochemical Study
Authors: Yip Ping K. , Hasan Shumaila , Liu Zhuo-Hao , Uff Christopher E. G.
Published: February 2022

Traumatic brain injury (TBI) is an extremely complex disease and current systems classifying TBI as mild, moderate, and severe often fail to capture this complexity. Neuroimaging cannot resolve the cellular and molecular changes due to lack of resolution, and post-mortem tissue examination may not adequately represent acute disease. Therefore, we examined the cellular and molecular sequelae of TBI in fresh brain samples and related these to clinical outcomes. Brain biopsies, obtained shortly after injury from 25 living adult patients suffering severe TBI, underwent immunohistochemical analysis. There were no adverse events. Immunostaining revealed various qualitative cellular and biomolecular changes relating to neuronal injury, dendritic injury, neurovascular injury, and neuroinflammation, which we classified into 4 subgroups for each injury type using the newly devised Yip, Hasan and Uff (YHU) grading system. Based on the Glasgow Outcome Scale-Extended, a total YHU grade of ≤8 or ≥11 had a favourable and unfavourable outcome, respectively. Biomolecular changes observed in fresh brain samples enabled classification of this heterogeneous patient population into various injury severity categories based on the cellular and molecular pathophysiology according to the YHU grading system, which correlated with outcome. This is the first study investigating the acute biomolecular response to TBI.
Study on the Perception Mechanism of Utricles Based on Bionic Models
Authors: Jiang Yani , Wang Xianjin , Lu Shien , Qin Yongbin , He Can , Bian Yixiang
Published: February 2022

Background: The relationship between utricle diseases and structural lesions is not very clear in the clinic due to the complexity and delicacy of the utricle structure. Therefore, it is necessary to study the perception mechanism of the utricle. Methods: Imitating the sensory cells in the macula of the utricle, a symmetrical metal core PVDF fiber (SMPF) was designed as a bionic hair sensor to fabricate a bionic macula (BM), a bionic macula with sand (BMS) and a bionic utricle (BU). Then experiments were carried out on them. Results: This indicated the SMPF sensor can sense its bending deformation, which was similar to the sensory cell. The amplitude of the output charges of the SMPF in BMS and BU were significantly improved. The SMPF, whose electrode boundary was perpendicular to the impact direction, exhibited the largest output charges. Conclusion: The presence of otoliths and endolymph can improve the sensing ability of the utricle. The human brain can judge the direction of head linear accelerations based on the location of the sensory cell in the macula that produces the largest nerve signals. This provides a possibility of studying utricle abnormal functions in vitro in the future.
Temperature Dependence of Hydrogen Adsorption on Pd-Modified Carbon Blacks and Their Enthalpy-Entropy Changes
Authors: Kaneko Takehiro , Toyama Takeshi , Kojima Yoshiyuki , Nishimiya Nobuyuki
Published: February 2022

Metal-carbon composites have recently gained attention as potential hydrogen storage materials. In the present investigation, carbon blacks (CBs) with 0.6 mass %, 4.9 mass %, and 9.3 mass % of Pd were prepared to investigate the cooperative effect together with Pd and CB for hydrogen storage. The hydrogen adsorption isotherms were measured at 77 K, 98 K, 123 K, 148 K, 173 K, 223 K, and 273 K under mild pressures below 1 MPa. The lower temperature gave the higher hydrogen content. Almost all the hydrogen contents of Pd-modified CBs exceeded the sum of the adsorption contents of CB and the occluded amounts of the assumed hydride, PdH0.6. The highest hydrogen content was recorded for Pd 0.6 mass %-modified CB at 77 K. At temperatures above 77 K, CBs with the higher Pd contents adsorbed more hydrogen than Pd 0.6 mass %-modified CB, and they indicated an increase in the absolute values of adsorption enthalpy with the progress of adsorption. Pd was thought to be at first blocking deep potential sites, with accessibility to hydrogen acceptable sites gradually increasing as adsorption progressed.
Therapeutic Strategies for Patients with Advanced Small Bowel Adenocarcinoma: Current Knowledge and Perspectives
Authors: Moati Emilie , Overman Michael J. , Zaanan Aziz
Published: February 2022

Small bowel adenocarcinoma (SBA) is diagnosed at an advanced (unresectable or metastatic) tumor stage in approximately one-third of cases. This is partly due to the non-specific symptomatology and limitations in endoscopic and radiologic detection methods. In this context, the prognosis remains poor and systemic chemotherapy appears to benefit patients when compared to best supportive care alone, despite the absence of randomized controlled trials. The results of a recent large prospective cohort (ARCAD-NADEGE) reported that the absence of chemotherapy was a predictive factor for a lower overall survival (OS) even though poor differentiation and SBA associated with Crohn’s disease correlate with poor prognosis. In retrospective series, the median OS ranges from approximately 9 to 18 months with current treatment approaches. A combination of a fluoropyrimidine and oxaliplatin (FOLFOX or CAPOX) appears to be the most utilized and effective first-line chemotherapy regimen. Other front-line alternatives are the combination of 5-FU and cisplatin or fluoropyrimidine and irinotecan (FOLFIRI). In second-line, FOLFIRI is an effective option after progression on platinum-based therapy. Taxane-based therapy appears to be an alternative option, but further evaluation in larger series is needed. To a limited extent, the role of surgical resection for metastatic disease appears to be a valid option, though this approach has not been evaluated in prospective clinical studies. Due to the rareness of the disease, inclusion in clinical trials should be prioritized, and there is hope that targeted therapies and immunotherapy may enter the therapeutic arsenal for these patients.