bluuebunny/crossref_metadata_2025 huggingface dataset:
Convergence of economic growth and health expenditures in OECD countries: Evidence from non-linear unit root tests
Authors: Celik Esref Ugur , Omay Tolga , Tengilimoglu Dilaver
Published: March 2023

Introduction

The relationship between human capital, health spending, and economic growth is frequently neglected in the literature. However, one of the main determinants of human capital is health expenditures, where human capital is one of the driving forces of growth. Consequently, health expenditures affect growth through this link.

Methods

In the study, these findings have been attempted to be empirically tested. Along this axis, health expenditure per qualified worker was chosen as an indicator of health expenditure, and output per qualified worker was chosen as an indicator of economic growth. The variables were treated with the convergence hypothesis. Due to the non-linear nature of the variables, the convergence hypothesis was carried out with non-linear unit root tests.

Results

The analysis of 22 OECD countries from 1976 to 2020 showed that health expenditure converged for all countries, and there was a significant degree of growth convergence (except for two countries). These findings show that health expenditure convergence has significantly contributed to growth convergence.

Discussion

Policymakers should consider the inclusiveness and effectiveness of health policies while making their economic policies, as health expenditure convergence can significantly impact growth convergence. Further research is needed to understand the mechanisms behind this relationship and identify specific health policies most effective in promoting economic growth.
Phase and wave dependent analysis of health expenditure efficiency: A sample of OECD evidence
Authors: Boduroglu Elif , Atici Kazim Baris , Omay Tolga
Published: March 2023

Introduction

Health expenditures are a factor that reflects the government's public health policy and contributes to the protection of national health. Therefore, this study focuses on measuring the effectiveness of health expenditures in order to evaluate and improve the public health system and policy during the pandemic period.

Method

In order to examine the effectiveness of health expenditures, the behaviors of the pandemic process were analyzed in two stages. The number of daily cases is analyzed in the first stage by dividing it into waves and phases according to the transmission coefficient (R). For this classification, the discrete cumulative Fourier function estimation is used. In the second stage, the unit root test method was used to estimate the stationarity of the number of cases in order to examine whether the countries made effective health expenditures according to waves and phases. The series being stationary indicates that the cases are predictable and that health expenditure is efficient. Data consists of daily cases from February 2020 to November 2021 for 5 OECD countries.

Conclusion

The general results are shown that cases cannot be predicted, especially in the first stage of the pandemic. In the relaxation phase and at the beginning of the second wave, the countries that were seriously affected by the epidemic started to control the number of cas es by taking adequate measures, thus increasing the efficiency of their health systems. The common feature of all the countries we examined is that phase 1, which represents the beginning of the waves, is not stationary. After the waves fade, it can be concluded that the stationary number of health cases cannot be sustainable in preventing new waves' formation. It is seen that countries cannot make effective health expenditures for each wave and stage. According to these findings, the periods in which countries made effective health expenditures during the pandemic are shown.

Discussion

The study aims to help countries make effective short- and long-term decisions about pandemics. The research provides a view of the effectiveness of health expenditures on the number of cases per day in 5 OECD countries during the COVID-19 Pandemic.
Multi-temporal high-resolution marsh vegetation mapping using unoccupied aircraft system remote sensing and machine learning
Authors: Windle Anna E. , Staver Lorie W. , Elmore Andrew J. , Scherer Stephanie , Keller Seth , Malmgren Ben , Silsbe Greg M.
Published: March 2023

Coastal wetlands are among the most productive ecosystems in the world and provide important ecosystem services related to improved water quality, carbon sequestration, and biodiversity. In many locations, wetlands are threatened by coastal development and rising sea levels, prompting an era of tidal wetland restoration. The creation and restoration of tidal marshes necessitate the need for ecosystem monitoring. While satellite remote sensing is a valuable monitoring tool; the spatial and temporal resolution of imagery often places operational constraints, especially in small or spatially complex environments. Unoccupied aircraft systems (UAS) are an emerging remote sensing platform that collects data with flexible on-demand capabilities at much greater spatial resolution than sensors on aircraft and satellites, and resultant imagery can be readily rendered in three dimensions through Structure from Motion (SfM) photogrammetric processing. In this study, UAS data at 5 cm resolution was collected at an engineered wetland at Poplar Island, located in Chesapeake Bay, MD United States five times throughout 2019 to 2022. The wetland is dominated by two vegetation species: Spartina alterniflora and Spartina patens that were originally planted in 2005 in low and high marsh elevation zones respectively. During each survey, UAS multispectral reflectance, canopy elevation, and texture were derived and used as input into supervised random forest classification models to classify species-specific marsh vegetation. Overall accuracy ranged from 97% to 99%, with texture and canopy elevation variables being the most important across all datasets. Random forest classifications were also applied to down-sampled UAS data which resulted in a decline in classification accuracy as spatial resolution decreased (pixels became larger), indicating the benefit of using ultra-high resolution imagery to accurately and precisely distinguish between wetland vegetation. High resolution vegetation classification maps were compared to the 2005 as-built planting plans, demonstrating significant changes in vegetation and potential instances of marsh migration. The amount of vegetation change in the high marsh zone positively correlated with interannual variations in local sea level, suggesting a feedback between vegetation and tidal inundation. This study demonstrates that UAS remote sensing has great potential to assist in large-scale estimates of vegetation changes and can improve restoration monitoring success.
Randomized controlled trial of motion-assisted exposure therapy for posttraumatic stress disorder after mild traumatic brain injury, with and without an eye movement task
Authors: Roy Michael J. , Bellini Paula , Kruger Sarah E. , Dunbar Kerri , Atallah Hannah , Haight Thaddeus , Vermetten Eric
Published: March 2023

Background and Purpose: PTSD and mTBI are persistent and frequently comorbid after combat, yet current therapies often achieve only modest impact. A novel exposure-based “walk and talk” cognitive therapy, Motion-Assisted, Multi-Modal Memory Desensitization and Reconsolidation (3MDR), featuring participant-selected music and pictures and an eye movement (EM) task in an immersive virtual environment, has shown efficacy in treatment-resistant male veterans, but has not been studied in women or after TBI. The EM task is adapted from eye movement desensitization and reprocessing (EMDR) therapy, but dismantling studies of EMDR have questioned EM benefit. This pilot study assesses 3MDR in male and female veterans with comorbid PTSD and mTBI, and the impact of EM on response. We hypothesized that 3MDR would prove efficacious, both with (EM+) and without EM (EM-).

Design: Participants with probable PTSD (PCL-5 ≥ 34) and mTBI were randomized to EM + or EM-across 10 sessions. Participants provided songs and pictures that they rated on impactfulness. While walking in the 3MDR virtual environment, participants started with a song to bring them back to the time of their trauma, and then traversed two hallways, actively walking toward emotionally evocative pictures that they then discussed with their therapist. Key words or feelings they expressed were superimposed over the picture, then read aloud, whereupon EM + participants recited numbers flashing on a ball crisscrossing the picture. These procedures were repeated for multiple pictures per session. A song to return the participant to present day closed each session. Change in PCL-5 score from pre-to post-intervention was the primary outcome, with additional measures at 3 and 6 months.

Results: Sixteen (80%) of 20 participants completed the intervention (8 EM+, 8 EM-); 9 (6 EM+, 3 EM-) had resolution of PTSD diagnosis and two improved significantly without resolution. Average PCL-5 score declined from 52.0 (95% confidence intervals: 46.3, 57.7) at baseline to 33.6 (24.3, 42.9) post-intervention (p < 0.01). The EM + group achieved statistically significant improvement (p = 0.01) while the EM-did not (p = 0.10).

Conclusion: For veterans with PTSD and comorbid mTBI, 3MDR is effective, and the EM component may add value. Confirmation with larger studies is important.
A short review on the ultra-high temperature mechanical properties of refractory high entropy alloys
Authors: Atli Kadri C. , Karaman I.
Published: March 2023

Mechanical properties of refractory high entropy alloys (RHEAs) at ultra-high temperatures (>1,100°C) are reviewed. Deformation behavior and strengthening mechanisms of select compositions are discussed. The limited number of studies portray remarkable mechanical properties of newly developed RHEA compositions at temperatures beyond the melting point of commercial Ni-based superalloys. Yet, the lack of quasi-static tensile deformation data and application relevant creep deformation data indicates RHEAs are still far from being reliable alternatives to Ni-based superalloys as high temperature structural materials. Future studies should concentrate on tensile deformation and creep of these new alloys systems at very high temperatures.
The impact of echinococcosis interventions on economic outcomes in Qinghai Province of China: Evidence from county-level panel data
Authors: Cai Jinshan , Yang Kefan , Chen Qihui , Zhao Quanbang , Li Jing , Wang Sen , Yang Lin , Liu Yumei
Published: March 2023

Introduction

Echinococcosis can incur substantial economic losses for the livestock industry by causing organ condemnation, delayed growth, and reduced meat and wool output and quality in sheep and cattle, as well as increased surgery costs, hospital care, and decreased productivity in humans. Yet echinococcosis could be prevented and controlled by interventions, such as dog management and deworming, lamb vaccination, slaughter management, and training and public education.

Methods

Exploiting temporal and spatial variations in the number of intervention measures implemented in 39 counties in Qinghai province of China in 2015–2020, this study assesses the economic impact of echinococcosis interventions using a dynamic difference-in-differences model.

Results

The results suggest that echinococcosis interventions brought about substantial economic gains measured by per capita net income of rural residents and per capita gross output of animal husbandry. These economic gains are greater in non-pastoral counties (with a gain in per capita net income of rural residents of 3,308 yuan and a gain per capita gross output of animal husbandry of 1,035 yuan) than in pastoral counties (with a gain in per capita net income of rural residents of 1,372 yuan and a gain per capita gross output of animal husbandry of 913 yuan). They are also greater in counties with echinococcosis infection level-2 (with a human infection rate of 0.1–1% or a dog infection rate of 1–5%) than infection level-1 counties (with a human prevalence rate ≥1% or a dog infection rate ≥5%).

Discussion

Not only will these economic gains encourage livestock farmers to strengthen their echinococcosis prevention and control practices, but they will also inform public policy on zoonotic disease prevention and control in China and other countries alike.
Case report: Composite mantle cell lymphoma and classical Hodgkin lymphoma
Authors: Wang Hongyu , Yang Liqun , Li Qiuyao , Song Haiyun , Ji Hong
Published: March 2023

Composite mantle cell lymphoma and classical Hodgkin lymphoma is very rare and the actual origin of it is still unclear. Here we reported a new case of composite mantle cell lymphoma and classical Hodgkin lymphoma and analyzed its molecular changes. Eight mutations were identified in its Hodgkin component through next-generation sequencing. In addition, we reviewed the published cases of composite mantle cell lymphoma and classical Hodgkin lymphoma and summarized the molecular changes of reported cases as well as the current case to explore the possible pathway of histogenesis.
Beyond Donations: Isomorphism and Revenue Mix in Nonprofit Start-Ups
Authors: Searing Elizabeth A. M.
Published: March 2023

The literature on nonprofit growth contains a practical ambiguity regarding which types of revenues to cultivate as a start-up nonprofit. The revenue portfolio of a more established organization may be inappropriate (or unattainable) for a new one, but there may be perils in relying too long on sources of nonprofit start-up capital. We posit that nonprofit entrepreneurs choose to mimic larger organizations in their field for growth rather than rely on the revenue mix of their start-up stage. This study uses two different dynamic econometric models to estimate the role of revenue type and other organizational factors in the growth of young and small nonprofits. We find that mimicking the revenue habits of larger organizations is generally (but not universally) advisable, with most conclusions sensitive to subsector.
Quality Detection Method of Penaeus vannamei Based on Lightweight YOLOv5s Network
Authors: Chen Yanyi , Huang Xuhong , Zhu Cunxin , Tang Shengping , Zhao Nan , Xiao Weihao
Published: March 2023

The appearance and meat quality of Penaeus vannamei are important indexes in the production process, and the quality of the product will be reduced if the defective shrimp is mixed in during processing. In order to solve this problem, a quality detection model of Penaeus vannamei based on deep learning was put forward. Firstly, the self-made dataset of Penaeus vannamei was expanded to enhance the generalization ability of the neural network. Secondly, the backbone of YOLOv5 (you only look once v5) is replaced by the lightweight network PP-LCNet that removes the dense layer at the end, which reduces the model parameters and calculation. Then, the 7 × 7 convolution DepthSepConv module is embedded in a PP-LCNet backbone, which effectively strengthens the feature extraction ability of the network. Ultimately, SiLU activation function is used to replace the Hardsigmoid and Hardswish activation functions in the PP-LCNet backbone to enhance the regularization ability and detection speed of the network. Through comparative experiments, the all-round performance of the Shrimp-YOLOv5s network is higher than the current mainstream classical model and the lightweight model. The mAP@0.5, mAP@0.5:0.95, detection speed, parameters, and calculation of Shrimp-YOLOv5s are 98.5%, 88.1%, 272.8 FPS (frames per second), 4.8 M, and 9.0 GFLOPs (giga floating point operations) respectively.
UAV-Based Remote Sensing for Soybean FVC, LCC, and Maturity Monitoring
Authors: Hu Jingyu , Yue Jibo , Xu Xin , Han Shaoyu , Sun Tong , Liu Yang , Feng Haikuan , Qiao Hongbo
Published: March 2023

Timely and accurate monitoring of fractional vegetation cover (FVC), leaf chlorophyll content (LCC), and maturity of breeding material are essential for breeding companies. This study aimed to estimate LCC and FVC on the basis of remote sensing and to monitor maturity on the basis of LCC and FVC distribution. We collected UAV-RGB images at key growth stages of soybean, namely, the podding (P1), early bulge (P2), peak bulge (P3), and maturity (P4) stages. Firstly, based on the above multi-period data, four regression techniques, namely, partial least squares regression (PLSR), multiple stepwise regression (MSR), random forest regression (RF), and Gaussian process regression (GPR), were used to estimate the LCC and FVC, respectively, and plot the images in combination with vegetation index (VI). Secondly, the LCC images of P3 (non-maturity) were used to detect LCC and FVC anomalies in soybean materials. The method was used to obtain the threshold values for soybean maturity monitoring. Additionally, the mature and immature regions of soybean were monitored at P4 (mature stage) by using the thresholds of P3-LCC. The LCC and FVC anomaly detection method for soybean material presents the image pixels as a histogram and gradually removes the anomalous values from the tails until the distribution approaches a normal distribution. Finally, the P4 mature region (obtained from the previous step) is extracted, and soybean harvest monitoring is carried out in this region using the LCC and FVC anomaly detection method for soybean material based on the P4-FVC image. Among the four regression models, GPR performed best at estimating LCC (R2: 0.84, RMSE: 3.99) and FVC (R2: 0.96, RMSE: 0.08). This process provides a reference for the FVC and LCC estimation of soybean at multiple growth stages; the P3-LCC images in combination with the LCC and FVC anomaly detection methods for soybean material were able to effectively monitor soybean maturation regions (overall accuracy of 0.988, mature accuracy of 0.951, immature accuracy of 0.987). In addition, the LCC thresholds obtained by P3 were also applied to P4 for soybean maturity monitoring (overall accuracy of 0.984, mature accuracy of 0.995, immature accuracy of 0.955); the LCC and FVC anomaly detection method for soybean material enabled accurate monitoring of soybean harvesting areas (overall accuracy of 0.981, mature accuracy of 0.987, harvested accuracy of 0.972). This study provides a new approach and technique for monitoring soybean maturity in breeding fields.
Systematic Evaluation of Nitrogen Application in the Production of Multiple Crops and Its Environmental Impacts in Fujian Province, China
Authors: Li Jun , Xing Jiali , Ding Rui , Shi Wenjiao , Shi Xiaoli , Wang Xiaoqing
Published: March 2023

Systematic evaluation of nitrogen (N) application in multi-crop production and its environmental impacts are of great significance for sustainable development of agriculture. Previous studies have focused on the evaluation of grain crops at the national and provincial levels, but ignored the county scale. Here, we evaluated the N rate, N use efficiency (NUE), N surplus, and greenhouse gas (GHG) emissions from the production of multiple crops including rice, wheat, maize, soybeans, tea, fruits, and vegetables at the county level of Fujian Province, China. The results showed that the N rates, N surpluses, and GHG emissions were generally higher, and NUEs were generally lower in the southern and southeastern coastal counties of Fujian Province, while the counties in the north and west had the opposite distribution trends. The N input and its negative environmental impacts for grain crops were generally lower than those for cash crops. The average NUE of all crops in Fujian Province in 2014 was 52.31%. The N input and N surplus for fruits accounted for 43.95% and 46.69% of those in the whole province, respectively. The evaluated framework we proposed in this study can be widely applied in the systematic evaluation of N input and its environmental footprints at the county scale for regions with multi-crop production.
Increasing the Biodiversity of the Dendroflora of Sparsely Wooded Regions by Adapted Representatives of the Genus Robinia L.
Authors: Kalmykova Elena , Lazarev Sergei
Published: March 2023

Purpose of the study: to substantiate the prospects of using various species, varieties and forms of the genus Robinia in protective afforestation and landscaping of settlements in dry-steppe and semi-desert zones of southern Russia. It is established that the main limiting factors affecting the growth, development and condition of representatives of the genus Robinia in the Volgograd region are winter temperatures up to −37 °C, as well as poor moisture availability and uneven distribution of precipitation during the growing season in combination with extremely low temperatures in the autumn–winter period. All representatives of the generic complex belong to the group of plants that start late and finish the vegetative period late. Phenological atypicity is in the lower half of the normal range, with indicators from +1 °C to 0, which indicates a high level of adaptation to the climatic conditions of the region. In the process of long-term acclimatization, many introduced species have developed a number of genotypic adaptations and are currently able to tolerate extreme winter temperatures up to −37 °C. An assessment of potential drought resistance based on the water-retaining ability of the leaves showed that higher rates of water-resistance capacity (76.8% water loss) are typical of R. neomexicana, which has a natural distribution area in the western arid part of the North American continent. R. viscosa var. hartwegii, with a natural distribution in the areas of the monsoon subtropical climate of eastern North America, is distinguished by low indicators (94.1% water loss), as are clonal decorative forms of R. pseudoacacia: f. pyramidalis and f. umbraculifera (97.6–95.8% water loss), which are common only in culture and characterized by a whole complex of low indicators of bioecological stability. The data obtained by us allow us to recommend the following assortment of species and forms of Robinia for protective afforestation and landscaping in the arid territories of southern Russia: R. pseudoacacia, R. neomexicana var. neomexicana, R. neomexicana var. rusbyi and R. pseudoacacia x R. neomexicana.
Impact of Electrocution on Shoot and Tuber Vitality of Yellow Nutsedge (Cyperus esculentus)
Authors: Feys Jeroen , De Cauwer Benny , Reheul Dirk , Sciffer Clara , Clercx Shana , Palmans Sander
Published: March 2023

Cyperus esculentus is an invasive perennial sedge that can cause huge losses in arable crops. Current control strategies are based on combinations of cultural, mechanical, and chemical measures, repeated over years. Recent commercial releases of safe innovative electric weeders, offer promising alternative opportunities for controlling perennial weeds with high energy/high frequency electricity. To evaluate the effect of a single electrocution application on the efficacy of C. esculentus control, field experiments were performed in two locations in Belgium. Two electric weeding devices were evaluated: Zasso XP300, delivering a high-frequency, phased direct current (maximum voltage of 7000 V and maximum power output of 2000 W per square meter of green biomass, driving speeds between 1.1 and 3.0 km·h−1), and Rootwave Pro, delivering high-frequency alternating current (maximum voltage of 5000 V and power output between 7.5 and 10 kVA, treatment duration of 2 s). The impact of various technical (driving speed and voltage), biotic (clone and growth stage), and abiotic parameters on electrocution efficacy was evaluated. Plant responses to electrocution were evaluated by examining the vitality of treated C. esculentus mother tubers and shoots. Both devices were ineffective at mother tuber control, regardless of their burial depth (−5 cm to −15 cm), but were highly effective against aboveground shoots with reductions of vitality of up to 91% and 100% after a single pass with Zasso XP300 and Rootwave Pro, respectively. Maximum reductions were obtained when electricity was delivered at low speed (1.1 to 1.5 km·h−1) and on 5-leaf shoots without heat or water stress. Remarkably, the lowest efficacies were found on water-stressed soils at the time of application. Voltage had no effect on the degree of C. esculentus control. The efficacy of electricity was not affected by clone, irrespective of electric weeding device. Electrocution is a useful and effective control method within any integrated control strategy for controlling emerged shoots. However, as C. esculentus mother tubers are not affected by a single treatment, season-long repeated treatments are needed to exhaust the mother tubers.
Physiological, Biochemical, Anatomical, and Agronomic Responses of Sesame to Exogenously Applied Polyamines under Different Irrigation Regimes
Authors: Desoky El Sayed M. , Alharbi Khadiga , Rady Mostafa M. , Elnahal Ahmed S. M. , Selem Eman , Arnaout Safaa M. A. I. , Mansour Elsayed
Published: March 2023

Polyamines (PAs) are plant growth regulators that attenuate the destructive impacts of water deficit on sesame plants, particularly in arid regions under abrupt climate change. Limited information is available on the physiological, biochemical, anatomical, and agronomic responses of sesame to exogenous application of PAs under water deficit under field conditions. Thus, a two-season field trial was carried out to assess the impact of exogenously sprayed spermine (Spm), spermidine (Spd), and putrescine (Put) on physio-biochemical and anatomical parameters and their influences on agronomic performance and crop water productivity of sesame plants. The sesame plants were assessed under three irrigation regimes; full irrigation (100% ETc), mild drought (75% ETc), and severe drought (50% ETc) conditions. Drought stress displayed negative impacts on all evaluated parameters. However, exogenously applied Pas, especially Put, restricted the destructive effects of water deficit. The application of PAs exhibited highly significant enhancement in photosynthetic pigments (chlorophyll a, b, and carotenoids), gas exchange (net photosynthetic rate, stomatal conductance, and rate of transpiration), water relations (relative water content, membrane stability index, excised leaf water retention), and plant nutrient content (N, P, and K) compared to untreated plants, particularly under severe drought stress. Moreover, PA application displayed highly significant amelioration in osmoprotectants (free proline, soluble sugars, α-Tocopherol, ascorbate, and glutathione), and antioxidant enzyme activities (catalase, peroxidase, superoxide dismutase, ascorbate peroxidase, and glutathione reductase). In addition, foliar nourishing with PAs exhibited a highly significant reduction in oxidative stress markers (malondialdehyde, electrolyte leakage, superoxide, and hydrogen peroxide). These positive impacts of PA application under drought stress were reflected in highly significant improvement in anatomical characteristics (midrib length and width, vascular bundle length and width, thickness of phloem, xylem and collenchyma, vessel diameter, and number of xylem rows in midvein bundle), and yield-related traits (plant height, leaf area, number of capsules per plant, 1000-seed weight, seed yield, and oil content). Consequently, exogenous application of PAs (in particular, Put) could be exploited to enhance the crop water productivity and yield traits of sesame plants under low water availability in arid regions.
An Endophytic Fungal Isolate Paecilomyces lilacinus Produces Bioactive Secondary Metabolites and Promotes Growth of Solanum lycopersicum under Heavy Metal Stress
Authors: Musa Muhammad , Jan Farzana Gul , Hamayun Muhammad , Jan Gul , Khan Sumera Afzal , Rehman Gauhar , Ali Sajid , Lee In-Jung
Published: March 2023

Heavy metal (HM) contamination in the soil and accumulation in plants poses a serious threat to crops and human health. HMs such as cadmium, mercury, lead and arsenic are widely acknowledged for their adverse effects on plants such as chlorosis, necrosis, low food quality and crop yields. Endophytic fungi secrete secondary metabolites and enhance the plant’s ability to tolerate stressful conditions. However, the role of most fungal endophytes in their host plant growth or production of metabolites under HM stress conditions needs further understanding. In the present study, we studied the HM stress alleviation capability of the endophytic fungus, Paecilomyces lilacinus (MRF), isolated from the roots of Justicia adathoda. We studied two heavy metals, namely lead and cobalt. The culture filtrate (CF) of P. lilacinus revealed IAA (68.17 µg/mL), phenols (43.31 µg/mL), flavonoids (40.59 µg/mL), sugar content (97.83 µg/mL) and proline (17 µg/mL). Additionally, DPPH-free radical scavenging activity and the antibacterial potential against Salmonella typhi and Shigella sonnei of the CF demonstrated positive results. The gas chromatography mass spectrometry analysis of the CF manifested different constituents, including (1) Trichloromethane, (2) 3,7,11,15-Tetramethyl-2-hexadecen-1-ol, (3) phytol, (4) 1,2-Benzenedicarboxylic acid, (5) bis(2-ethylhexyl) ester, (6) squalene and (7) Cyclotrisiloxane Hexamethyl. Moreover, the plant growth-promoting activity of the P. lilacinus (MRF) strain revealed a robust increase in root and shoot growth, and the fresh and dry weight of S. lycoprsicum. Further, the IAA, phenols, flavonoids, sugar, proline, relative water content and protein contents also increased in the S. lycoprsicum inoculated with P. lilacinus as compared to the control plants. The present study revealed that the inoculation of P. lilacinus alleviates the damages of HM stress and improves the physicochemical characteristics of S. lycoprsicum.
Accumulation of Labile P Forms and Promotion of Microbial Community Diversity in Mollisol with Long-Term Manure Fertilization
Authors: Song Shuhui , Zhang Jinyao , Liu Yunxia , Wang Hong
Published: March 2023

Soil phosphorus (P) can be divided into inorganic P (Pi) and organic P (Po). Microorganisms play essential roles in soil P transformation. However, there are many ways to detect P transformation, and the relationship between P forms and microorganisms under long-term fertilization is largely unclear. In this study, soil P forms were analyzed by a chemical sequential fractionation method and solution 31P nuclear magnetic resonance (31P-NMR) technique. Phospholipid fatty acid (PLFA) contents were measured by gas chromatography as the characterization of soil microbial community structures. The objective was to determine the changes of soil P forms and associated microbial community composition in mollisol with long-term fertilization. We sampled soil from a field experiment with 26-year-old continuous maize (Zea mays L.) cropping in Northeastern China. Three fertilization treatments were selected as chemical fertilization (NPK), NPK with crop straw (NPKS), and NPK with manure (NPKM). As shown in 31P-NMR spectra, orthophosphate accounted for 62.8–85.8% of total extract P. Comparison to NPK and NPKS treatments, NPKM application notably increased the concentrations of Po, Olsen-P, orthophosphate, orthophosphate monoester, and total P. Soil P fractions including resin-Pi, NaHCO3-P, NaOH-P, and HCl-P, especially Pi fractions, were enhanced by NPKM. The amounts of total PLFAs and PLFAs in bacteria, Gram-positive (G+) and Gram-negative (G−) bacteria, actinomycetes, and fungi were high in NPKM-treated soil. The percentages of PLFAs in bacteria and fungi in total soil PLFAs were 56.8% and 9.7%, respectively, which did not show any significant difference among the treatments. NPKM increased the proportions (%) of PLFAs in G+ bacteria, and NPKS increased the proportions (%) of G− bacteria in total PLFAs. The composition of soil microbial community was found to be significantly affected by soil total carbon and pH. There was a close relationship between HCl-Pi, NaHCO3-Po, orthophosphate, and pyrophosphate with anaerobe, aerobes, and G+. Manure addition directly increased soil available P concentrations, and indirectly acted through the alterations of anaerobe, aerobes, and G+. It is concluded that long-term NPKM application would lead to the accumulation of labile P and moderately labile P in mollisol through the activity of soil microbes.
Influence of Environmental Factors on Some Biochemical and Physiological Indicators in Grapevine from Copou Vineyard, Iasi, Romania
Authors: Marta Alina Elena , Slabu Cristina , Covasa Mihaela , Motrescu Iuliana , Lungoci Constantin , Jitareanu Carmenica Doina
Published: March 2023

Climate factors strongly impact the growth of grapes and their flavonoid composition, especially due to heat and drought stress. Four varieties—Gelu, Moldova, Purpuriu, and Coarna Neagra—from a Copou vineyard were analyzed to assess the impact of climate change on the anthocyanin concentration in grapes and total chlorophyll content of the leaves, and find possible correlations between these parameters during the two studied years, such as to raise producer awareness regarding the climate impact on table grape vine growth. Moldova and Purpuriu show adaptation to a slight temperature increase above the normal average and also to a water deficit, with increased concentrations of anthocyanins of up to three times. The Moldova variety accumulated low amounts of chlorophyll pigments in most analyzed development phases, indicating a negative correlation between these pigments. Coarna neagra exhibited an increase of the foliar pigments, a variety that proved to be less valuable from the point of view of anthocyanin content. Positive correlations were found between the anthocyanin and chlorophyll concentrations in the case of Gelu, Moldova, and Coarna neagra cultivars during the latter phase of the fruit development, whereas the water deficit in the second year induced negative correlations during fruit development and at the time of harvest for all studied varieties except Moldova.
Integrating SAR Sentinel-1A and DSSAT CROPGRO Simulation Model for Peanut Yield Gap Analysis
Authors: Thirumeninathan Subramanian , Pazhanivelan Sellaperumal , Sudarmanian N. S. , Ragunath Kaliaperumal , Kumaraperumal Ramalingam , Srinivasan Govindasamy , Mohan Ramalingam
Published: March 2023

Crop yield data are critical for managing agricultural sustainability and assessing national food security. This study aims at increasing peanut productivity from its current levels by analyzing the yield gap (difference) of potential production between theoretical yield and actual farmers’ yields. The spatial yield gap of peanut for the Tiruvannamalai district of Tamil Nadu is examined in this investigation by integrating the products of microwave remote sensing (SAR Sentinel-1A) with the DSSAT CROPGRO Peanut simulation model. The CROPGRO (crop growth) Peanut model was calibrated and validated by conducting a field experiment at Oilseeds Research Station, Tindivanam during Rabi (spring) 2019 for predominant cultivars, i.e., TMV 7, TMV 13, VRI 2 and G 7. Actual attainable yield was recorded by organizing crop cutting experiments (CCEs) with the help of the Department of Agriculture Economics and Statistics in the respective monitoring villages. The regression analysis between the maximum recorded DSSAT leaf area index (LAI) at the peak flowering stage of peanut and the yield recorded by CCEs for the spatial yield estimation of peanut in the Tiruvannamalai district of Tamil Nadu during Rabi 2021 was carried out using ArcGIS 10.6 software. The DSSAT CROPGRO simulated potential yield ranged from 3194 to 4843 kg/ha, whereas actual yield ranged from 1228 to 3106 kg/ha, with a considerable disparity between the actual and potential yield levels (from 1217 to 2346 kg/ha) of the monitored locations. The minimum, maximum and average yield gaps in peanut for Tiruvannamalai district were assessed as 1890, 2324 and 2134 kg/ha, respectively. In order to reduce the production difference of peanut cultivation, farmers should focus more on management issues such as time of sowing, irrigation or water management, quantity and sources of nutrients, cultivar selection and availability of quality seeds tailored to each region.
Accumulation of Polyphenolics and Differential Expression of Genes Related to Shikimate Pathway during Fruit Development and Maturation of Chinese Olive (Canarium album)
Authors: Cai Jingrong , Wang Naiyu , Zhao Junyue , Zhao Yan , Xu Rong , Fu Fanghao , Pan Tengfei , Yu Yuan , Guo Zhixiong , She Wenqin
Published: March 2023

Phenolics in the Chinese olive (Canarium album (Lour.) Raeusch) fruit significantly affect its flavor and quality. The shikimate pathway is a bridge connecting primary metabolism and secondary metabolism through which fixed carbon can be transformed into phenolics. In this study, we aimed to reveal the relationship between the shikimate pathway and phenolic compound biosynthesis. Three Chinese olive fruits (cv. Tanxiang (TX), Changying (CY) and Lingfeng (LF)) with distinct flavor were utilized as materials. The results of this study showed that the synthesis and accumulation of quinate and gallate were active in the Chinese olive fruit. The accumulation amount of phenolic compounds was significantly different among the three cultivars. TX contained the highest content of ellagate, (iso)corilagin, conjugated quercetin and conjugated kaempferol; CY contained the highest content of conjugated luteolin; and LF contained the lowest content of ellagate, conjugated gallate, hyperin, conjugated quercetin, conjugated kaempferol and conjugated luteolin during fruit development. The expression of 3-dehydroquinate/shikimate dehydrogenase gene-4 (DHD/SDH-4), 3-dehydroquinate synthase gene (DHQS), chorismate synthase gene (CS) and Chorismate mutase gene-1 (CM-1) and shikimate content increased with the maturing of fruit. The gene 3-Deoxy-D-arabino-heptulosonate-7-phosphate synthase gene-1 (DAHPS-1) was most expressed in TX, while barely expressed in LF during fruit development. The expression of CM-1 was highest in CY. Chorismate mutase gene-2 (CM-2) expression was higher in TX and CY during late fruit development. The cultivars with higher expression of DAHPS-1 and Chorismate mutase genes (CMs) accumulated more phenolic compounds in fruit. DAHPS-1 and CMs are proposed as key genes for polyphenolic synthesis in the Chinese olive fruit. These results proved that shikimate metabolism had a positive effect on the phenols’ synthesis. Our study provides new insight into the regulatory mechanism of the biosynthesis and accumulation of phenolic compounds in the fruit of Chinese olive.
Advances in Simple and Chiral-HPLC Methods for Antiallergic Drugs and Chiral Recognition Mechanism
Authors: Ali Imran , Alam Syed Dilshad , Raja Rupak , Khan Shafat Ahmad , Anjum Rushda , Jain Arvind Kumar , Aboul-Enein Hassan Y. , Locatelli Marcello
Published: March 2023

Among many diseases, allergy appears to be a serious problem for human beings. Various forms of allergic disorders make people tense, leading to some other health issues. Many medications, including nonracemic and racemic ones, are used to treat this problem. It is important to have exact analysis strategies just to see any medication side effects, plasma profiles, and working efficiency. Therefore, efforts are made to review simple and chiral HPLC methods for antiallergic drugs; HPLC is the best analytical technique. The highlights in this article include the world scenario, causes of allergy, the effect of allergy on the economy, the mechanism of allergy in humans, classes of antiallergic drugs, simple drugs, chiral drugs, analysis by HPLC, and the chiral recognition mechanism. Moreover, attempts are also made to highlight the management of allergies and future perspectives.
Trace Elements and Persistent Organic Pollutants in Unhatched Loggerhead Turtle Eggs from an Emerging Nesting Site along the Southwestern Coasts of Italy, Western Mediterranean Sea
Authors: Esposito Mauro , Canzanella Silvia , Iaccarino Doriana , Pepe Angela , Di Nocera Fabio , Bruno Teresa , Marigliano Laura , Sansone Donato , Hochscheid Sandra , Gallo Pasquale , Maffucci Fulvio
Published: March 2023

Marine pollution is one of the major threats affecting loggerhead turtles, which due to their long life span, highly migratory behavior, and carnivorous diet, may be exposed to elevated levels of toxic elements throughout their life. The transfer of chemicals from mothers to their offspring is of particular conservation concern because it may affect embryonic development and hatching success. In this study, the concentrations of 16 toxic and potentially toxic trace elements, 6 indicator polychlorinated biphenyls (PCBs), and organochlorine pesticide residues (OCPs) were determined in 138 eggs from 46 loggerhead turtle nests laid during the 2021 nesting season in Campania, Italy, western Mediterranean Sea. The possible impact of pollutant levels on hatching success and early embryonic death was also investigated. Trace element analysis was performed using an ICP-MS, except for mercury, which was determined using a Direct Mercury Analyzer® (DMA). PCBs and OCPs were analyzed with high-resolution gas chromatography coupled with high-resolution mass spectrometry (HRGC-HRMS) and gas chromatography tandem quadrupole mass spectrometry GC-MS /MS, respectively. The concentrations of essential elements in the eggs were higher than those of non-essential elements. In addition, the highly chlorinated PCBs (153, 138, and 180) contributed the most to the total PCBs, while OCPs were not detected. No correlations were found between contaminant concentrations and reproductive parameters (hatching success and no obvious embryos). The results obtained suggest that the levels of contaminants found in the eggs do not affect the reproductive success of the species in the study area.
Knockout of Rlim Results in a Sex Ratio Shift toward Males but Superovulation Cannot Compensate for the Reduced Litter Size
Authors: Peng Jingfeng , Hou Yunfei , Wu Shici , Li Zicong , Wu Zhenfang
Published: March 2023

Technologies that can preselect offspring gender hold great promise for improving farm animal productivity and preventing human sex-related hereditary diseases. The maternal Rlim allele is required for imprinted X-chromosome inactivation, which is essential for the normal development of female mouse embryos. In this study, we inactivated the maternal Rlim allele in embryos by crossing a male transgenic mouse line carrying an X-linked CMV-Cre transgene with a female line carrying a loxP-flanked Rlim gene. Knockout of the maternal Rlim gene in embryos resulted in a male-biased sex ratio skew in the offspring. However, it also reduced litter size, and this effect was not compensated for by superovulation in the mother mice. In addition, we showed that siRNA-mediated knockdown of Rlim in mouse embryos leads to the birth of male-only progenies. This study provides a new promising method for male-biased sex selection, which may help to improve the productivity in livestock and prevent sex-associated hereditary diseases in humans.
Intracellular Antibodies for Drug Discovery and as Drugs of the Future
Authors: Rabbitts T. H.
Published: March 2023

The application of antibodies in cells was first shown in the early 1990s, and subsequently, the field of intracellular antibodies has expanded to encompass antibody fragments and their use in target validation and as engineered molecules that can be fused to moieties (referred to as warheads) to replace the Fc effector region of a whole immunoglobulin to elicit intracellular responses, such as cell death pathways or protein degradation. These various forms of intracellular antibodies have largely been used as research tools to investigate function within cells by perturbing protein activity. New applications of such molecules are on the horizon, namely their use as drugs per se and as templates for small-molecule drug discovery. The former is a potential new pharmacology that could harness the power and flexibility of molecular biology to generate new classes of drugs (herein referred to as macrodrugs when used in the context of disease control). Delivery of engineered intracellular antibodies, and other antigen-binding macromolecules formats, into cells to produce a therapeutic effect could be applied to any therapeutic area where regulation, degradation or other kinds of manipulation of target proteins can produce a therapeutic effect. Further, employing single-domain antibody fragments as competitors in small-molecule screening has been shown to enable identification of drug hits from diverse chemical libraries. Compounds selected in this way can mimic the effects of the intracellular antibodies that have been used for target validation. The capability of intracellular antibodies to discriminate between closely related proteins lends a new dimension to drug screening and drug development.
Is Education Alone Enough to Sustain Improvements of Antimicrobial Stewardship in General Practice in Australia? Results of an Intervention Follow-Up Study
Authors: Sangwan Robin , Neels Alicia J. , Gwini Stella May , Saha Sajal K. , Athan Eugene
Published: March 2023

Sustained behaviour change and practice improvements for the optimal use of antimicrobials remains challenging in primary care. In 2018, a simple antimicrobial stewardship education programme involving guideline recommendations for common infections, antimicrobial audit reports, and local antibiograms resulted in significant improvements in guideline compliance and more appropriate antimicrobial prescribing by GPs. This observational follow-up study aims to examine the sustainability of the positive intervention effect after two years of implementation of the intervention. Practice-based data on all oral antimicrobial prescriptions issued by GPs were collected retrospectively to compare with intervention data and to measure the sustainability of the intervention effect. The data were analysed using a two-sample test of proportions. The primary outcomes included changes in the rate of prescription compliance with the Australian “Therapeutic Guidelines: Antibiotic” and the appropriateness of antimicrobial choice and duration of therapy. Overall, there was a significant decline in guideline compliance, from 58.5 to 36.5% (risk ratio (RR) (95% CI): 0.62 (0.52–0.74)), in the appropriateness of antimicrobial choice, from 92.8 to 72.8% (0.78 (0.73, 0.84)), and in the prescribed duration, from 87.7 to 53.3% (0.61 (0.54, 0.68)) in the intervention follow-up period. In respiratory infections and ear, nose, and throat infections, the rates of guideline compliance and appropriate choice and duration of antimicrobial prescription decreased significantly at p < 0.001. Appropriateness in the duration of antimicrobial therapy also significantly decreased for most antimicrobials. The evidence suggests that a simple and single-occasion antimicrobial stewardship education programme is probably not enough to sustain improvements in the optimal use of antimicrobials by GPs. Future research is needed to validate the results in multiple GP clinics and to examine the effect of sustained education programmes involving infection-specific and antimicrobial-targeted audits and feedback.