bluuebunny/crossref_metadata_2025 huggingface dataset:
Storm-Driven Fresh Submarine Groundwater Discharge and Nutrient Fluxes From a Barrier Island
Authors: Adyasari Dini , Montiel Daniel , Mortazavi Behzad , Dimova Natasha
Published: July 2021

Quantifying and characterizing groundwater flow and discharge from barrier islands to coastal waters is crucial for assessing freshwater resources and contaminant transport to the ocean. In this study, we examined the groundwater hydrological response, discharge, and associated nutrient fluxes in Dauphin Island, a barrier island located in the northeastern Gulf of Mexico. We employed radon (222Rn) and radium (Ra) isotopes as tracers to evaluate the temporal and spatial variability of fresh and recirculated submarine groundwater discharge (SGD) in the nearshore waters. The results from a 40-day continuous 222Rn time series conducted during a rainy season suggest that the coastal area surrounding Dauphin Island was river-dominated in the days after storm events. Groundwater response was detected about 1 week after the precipitation and peak river discharge. During the period when SGD was a factor in the nutrient budget of the coastal area, the total SGD rates were as high as 1.36 m day–1, or almost three times higher than detected fluxes during the river-dominated period. We found from a three-endmember Ra mixing model that most of the SGD from the barrier island was composed of fresh groundwater. SGD was driven by marine and terrestrial forces, and focused on the southeastern part of the island. We observed spatial variability of nutrients in the subterranean estuary across this part of the island. Reduced nitrogen (i.e., NH4+ and dissolved organic nitrogen) fluxes dominated the eastern shore with average rates of 4.88 and 5.20 mmol m–2 day–1, respectively. In contrast, NO3– was prevalent along the south-central shore, which has significant tourism developments. The contrasting nutrient dynamics resulted in N- and P-limited coastal water in the different parts of the island. This study emphasizes the importance of understanding groundwater flow and dynamics in barrier islands, particularly those urbanized, prone to storm events, or located near large estuaries.
Biogeography and Connectivity Across Habitat Types and Geographical Scales in Pacific Abyssal Scavenging Amphipods
Authors: Bribiesca-Contreras Guadalupe , Dahlgren Thomas G. , Horton Tammy , Drazen Jeffrey C. , Drennan Regan , Jones Daniel O.B. , Leitner Astrid B. , McQuaid Kirsty A. , Smith Craig R. , Taboada Sergio , Wiklund Helena , Glover Adrian G.
Published: July 2021

Recently, there has been a resurgent interest in the exploration of deep-sea mineral deposits, particularly polymetallic nodules in the Clarion-Clipperton Zone (CCZ), central Pacific. Accurate environmental impact assessment is critical to the effective management of a new industry and depends on a sound understanding of species taxonomy, biogeography, and connectivity across a range of scales. Connectivity is a particularly important parameter in determining ecosystem resilience, as it helps to define the ability of a system to recover post-impact. Scavenging amphipods in the superfamilies Alicelloidea Lowry and De Broyer, 2008 and Lysianassoidea Dana, 1849 contribute to a unique and abundant scavenging community in abyssal ecosystems. They are relatively easy to sample and in recent years have become the target of several molecular and taxonomic studies, but are poorly studied in the CCZ. Here, a molecular approach is used to identify and delimit species, and to investigate evolutionary relationships of scavenging amphipods from both abyssal plain and deep (>3000 m) seamount habitats in three APEIs (Areas of Particular Environmental Interest, i.e., designated conservation areas) in the western CCZ. A total of 17 different morphospecies of scavenging amphipods were identified, which include at least 30 genetic species delimited by a fragment of the cytochrome c oxidase subunit I (COI) barcode gene. The scavenging communities sampled in the western CCZ included the most common species (Abyssorchomene gerulicorbis (Shulenberger and Barnard, 1976), A. chevreuxi (Stebbing, 1906), Paralicella caperesca Shulenberger and Barnard, 1976, and P. tenuipes Chevreux, 1908) reported for other ocean basins. Only four morphospecies, representing five genetic species, were shared between APEIs 1, 4, and 7. The two abyssal plain sites at APEIs 4 and 7 were dominated by two and three of the most common scavenging species, respectively, while the APEI 1 seamount site was dominated by two species potentially new to science that appeared to be endemic to the site. The presence of common species in all sites and high genetic diversity, yet little geographic structuring, indicate connectivity over evolutionary time scales between the areas, which span about 1500 km. Similar to recent studies, the differences in amphipod assemblages found between the seamount and abyssal sites suggest that ecological conditions on seamounts generate distinct community compositions.
Non-invasive Diagnostic Tests in Cystic Fibrosis-Related Liver Disease: A Diagnostic Test Accuracy Network Meta-Analysis
Authors: Martonosi Ágnes Rita , Soós Alexandra , Rumbus Zoltán , Hegyi Péter , Izsák Vera , Pázmány Piroska , Imrei Marcell , Váncsa Szilárd , Szakács Zsolt , Párniczky Andrea
Published: July 2021

Background and Aims: Cystic fibrosis-related liver disease (CFLD) is one of the leading causes of morbidity and mortality in cystic fibrosis (CF). Several non-invasive diagnostic methods have been proposed as screening tools for CFLD. Our aim was to rank all available non-invasive modalities for diagnostic performance.

Methods: A systematic search was performed in five medical databases to find studies which reported on any single or composite non-invasive diagnostic test (as an index test) compared to the Debray, the EuroCare or the Colombo criteria (as a reference standard). Ranking was carried out with a Bayesian diagnostic test accuracy network meta-analysis based on superiority indices, calculated for pooled sensitivity (Se) and specificity (Sp) with a 95% confidence interval (CI). The study was registered under CRD42020155846 in PROSPERO.

Results: Fifteen studies with 15 index tests and a combination of them were included. The New criteria proposed by Koh et al. – which represent a composite diagnostic definition for CFLD including liver biochemistry, ultrasonography, transient elastography and fibrosis markers—had the best performance for detecting CFLD (Se:94%[CI:58–100], Sp:72%[CI:52–84]); while transient elastography (Se:65%[CI:56–74], Sp:88%[CI:84–91]) and a combination of it with a tissue inhibitor of metalloproteinase-4 measurement (Se:78%[CI:30–100], Sp:64%[CI:18–95%]) proved to be the second and third best options, respectively. In the imaging techniques subgroup, transient elastography (Se:66%[CI:57–72], Sp:88%[CI:85–91%]), acoustic radiation force impulse in the right lobe (Se:54%[CI:33–74], Sp:88%[CI:66–96]) and that in the left lobe (Se:55%[CI:23–81], Sp:82%[CI:50–95]) were ranked the highest. Comparing biochemical markers/fibrosis indices, the measurement of the Forns index (Se:72%[CI:25–99], Sp:63%[CI:16–94]), the aspartate aminotransferase-to-platelet ratio (Se:55%[CI:41–68], Sp:83%[CI:66–89]) and alkaline phosphatase (Se:63%[CI:18–93], Sp:64%[CI:19–95]) were ranked the highest.

Conclusion: The New criteria show the best diagnostic performance. In clinical practice, transient elastography seems to be a simple, cheap and non-invasive tool, outperforming imaging, biochemical and fibrosis tests for detecting CFLD. Further studies are needed to validate our findings.
Efficacy and Safety of Remimazolam for Procedural Sedation: A Meta-Analysis of Randomized Controlled Trials With Trial Sequential Analysis
Authors: Jhuang Bo-Jyun , Yeh Bo-Han , Huang Yen-Ta , Lai Pei-Chun
Published: July 2021

Background: Remimazolam is a new ultrashort-acting benzodiazepine. Remimazolam has been approved for procedural sedation by the US Food and Drug Administration in 2020. However, prior trials and the participants they enrolled were limited.

Aim: In this meta-analysis, we investigated the effectiveness and adverse events (AEs) of remimazolam during procedural sedation.

Materials and Methods: The study protocol was registered (doi: 10.37766/inplasy2020.8.0043), and six databases were searched. We performed meta-analysis, trial sequential analysis (TSA), and Grading of Recommendations, Assessment, Development, and Evaluation (GRADE) methodology for judging the certainty of evidence (CoE).

Results: A total of five randomized controlled trials with 1,248 participants were included. Compared with the use of midazolam, the utilization of remimazolam resulted in an increase in procedure success rate [odds ratio (OR) = 9.01, 95% confidence interval (CI): 2.35–34.57], a reduction in the application of rescue medication (OR = 13.58, 95% CI: 3.46–53.28), a decrease in time to recovery [minutes, weighted mean difference (WMD) = −5.70, 95% CI: −8.68 to −2.72], and a better cognitive recovery of Hopkins Verbal Learning Test-Revised (WMD = 5.22, 95% CI: 2.88–7.55). No difference was found in completion of procedure (OR = 1.68, 95% CI: 0.72–3.90) with inconclusive in TSA. Despite no difference of total AEs (OR = 0.60, 95% CI: 0.24–1.50), more detailed analysis of AEs remained inconclusive in TSA. The GRADE assessment demonstrated low to very low CoE.

Conclusion: Our analysis suggested that remimazolam may be a better choice for procedural sedation than midazolam. Nevertheless, further studies are warranted to conclusively establish its safety.
Non-RBM Mutations Impaired SARS-CoV-2 Spike Protein Regulated to the ACE2 Receptor Based on Molecular Dynamic Simulation
Authors: Du Yaoqiang , Wang Hao , Chen Linjie , Fang Quan , Zhang Biqin , Jiang Luxi , Wu Zhaoyu , Yang Yexiaoqing , Zhou Ying , Chen Bingyu , Lyu Jianxin , Wang Zhen
Published: July 2021

The emergence of novel coronavirus mutants is a main factor behind the deterioration of the epidemic situation. Further studies into the pathogenicity of these mutants are thus urgently needed. Binding of the spinous protein receptor binding domain (RBD) of SARS-CoV-2 to the angiotensin-converting enzyme 2 (ACE2) receptor was shown to initiate coronavirus entry into host cells and lead to their infection. The receptor-binding motif (RBM, 438–506) is a region that directly interacts with ACE2 receptor in the RBD and plays a crucial role in determining affinity. To unravel how mutations in the non-RBM regions impact the interaction between RBD and ACE2, we selected three non-RBM mutant systems (N354D, D364Y, and V367F) from the documented clinical cases, and the Q498A mutant system located in the RBM region served as the control. Molecular dynamics simulation was conducted on the mutant systems and the wild-type (WT) system, and verified experiments also performed. Non-RBM mutations have been shown not only to change conformation of the RBM region but also to significantly influence its hydrogen bonding and hydrophobic interactions. In particular, the D364Y and V367F systems showed a higher affinity for ACE2 owing to their electrostatic interactions and polar solvation energy changes. In addition, although the binding free energy at this point increased after the mutation of N354D, the conformation of the random coil (Pro384-Asp389) was looser than that of other systems, and the combined effect weakened the binding free energy between RBD and ACE2. Interestingly, we also found a random coil (Ala475-Gly485). This random coil is very sensitive to mutations, and both types of mutations increase the binding free energy of residues in this region. We found that the binding loop (Tyr495-Tyr505) in the RBD domain strongly binds to Lys353, an important residue of the ACE2 domain previously identified. The binding free energy of the non-RBM mutant group at the binding loop had positive and negative changes, and these changes were more obvious than that of the Q498A system. The results of this study elucidate the effect of non-RBM mutation on ACE2-RBD binding, and provide new insights for SARS-CoV-2 mutation research.
Multiple Roles of Brd4 in the Infectious Cycle of Human Papillomaviruses
Authors: McBride Alison A. , Warburton Alix , Khurana Simran
Published: July 2021

Human Papillomaviruses (HPV) reproduce in stratified epithelia by establishing a reservoir of low- level infection in the dividing basal cells and restricting the production of viral particles to terminally differentiated cells. These small DNA viruses hijack pivotal cellular processes and pathways to support the persistent infectious cycle. One cellular factor that is key to multiple stages of viral replication and transcription is the BET (bromodomain and extra-terminal domain) protein, Brd4 (Bromodomain containing protein 4). Here we provide an overview of the multiple interactions of Brd4 that occur throughout the HPV infectious cycle.
Presurgical Screening Improves Risk Prediction for Delirium in Elective Surgery of Older Patients: The PAWEL RISK Study
Authors: Eschweiler Gerhard W. , Czornik Manuel , Herrmann Matthias L. , Knauer Yvonne P. , Forkavets Oksana , von Arnim Christine A. F. , Denkinger Michael , Küster Olivia , Conzelmann Lars , Metz Brigitte R. , Maurer Christoph , Kentischer Felix , Deeken Friederike , Sánchez Alba , Wagner Sören , Mennig Eva , Thomas Christine , Rapp Michael A.
Published: July 2021

Introduction: The number of elective surgeries for patients who are over 70 years of age is continuously growing. At the same time, postoperative delirium (POD) is common in older patients (5–60%) depending on predisposing risk factors, such as multimorbidity, cognitive impairment, neurodegenerative disorders and other dementing disorders, and precipitating factors, such as duration of surgery. Knowledge of individual risk profiles prior to elective surgery may help to identify patients at increased risk for development of POD. In this study, clinical and cognitive risk factors for POD were investigated in patients undergoing various elective cardiac and non-cardiac surgeries.

Methods: The PAWEL study is a prospective, interventional trial on delirium prevention. At baseline, 880 inpatients at five surgical centers were recruited for sub-sample PAWEL-R. Multimodal assessments included clinical renal function, medication, American Society of Anesthesiologists (ASA) Physical Status Classification System, geriatric and cognitive assessments, which comprised the Montreal Cognitive Assessment Scale (MoCA), Trail-making Test, and Digit Span backward. Delirium incidence was monitored postoperatively by the Confusion Assessment Method (CAM) and a chart review for up to a week or until discharge. Multivariate regression models and Chi-square Automatic Interaction Detectors (CHAID) analyses were performed using delirium incidence as the primary outcome.

Results: Eighteen risk factors were investigated in elective cardiovascular and orthopedic or general surgery. A total of 208 out of 880 patients (24%) developed POD. A global regression model that included all risk variables predicted delirium incidence with high accuracy (AUC = 0.81; 95% CI 0.77, 0.85). A simpler model (clinical and cognitive variables; model CLIN-COG) of 10 factors that only included surgery type, multimorbidity, renal failure, polypharmacy, ASA, cut-to-suture time, and cognition (MoCA, Digit Span backward, and preexisting dementia), however, exhibited similar predictive accuracy (AUC = 0.80; 95% CI 0.76, 0.84).

Conclusion: The risk of developing POD can be estimated by preoperative assessments, such as ASA classification, expected cut-to-suture time, and short cognitive screenings. This rather efficient approach predicted POD risk over all types of surgery. Thus, a basic risk assessment including a cognitive screen can help to stratify patients at low, medium, or high POD risk to provide targeted prevention and/or management strategies for patients at risk.
Early Predictors of the Increase in Perihematomal Edema Volume After Intracerebral Hemorrhage: A Retrospective Analysis From the Risa-MIS-ICH Study
Authors: Ye Gengzhao , Huang Shuna , Chen Renlong , Zheng Yan , Huang Wei , Gao Zhuyu , Cai Lueming , Zhao Mingpei , Ma Ke , He Qiu , Lin Fuxin , Lin Yuanxiang , Wang Dengliang , Fang Wenhua , Kang Dezhi , Wu Xiyue
Published: July 2021

Background and Purpose: Perihematomal edema (PHE) is associated with poor functional outcomes after intracerebral hemorrhage (ICH). Early identification of risk factors associated with PHE growth may allow for targeted therapeutic interventions.

Methods: We used data contained in the risk stratification and minimally invasive surgery in acute intracerebral hemorrhage (Risa-MIS-ICH) patients: a prospective multicenter cohort study. Patients' clinical, laboratory, and radiological data within 24 h of admission were obtained from their medical records. The absolute increase in PHE volume from baseline to day 3 was defined as iPHE volume. Poor outcome was defined as modified Rankin Scale (mRS) of 4 to 6 at 90 days. Binary logistic regression was used to assess the relationship between iPHE volume and poor outcome. The receiver operating characteristic curve was used to find the best cutoff. Linear regression was used to identify variables associated with iPHE volume (ClinicalTrials.gov Identifier: NCT03862729).

Results: One hundred ninety-seven patients were included in this study. iPHE volume was significantly associated with poor outcome [P = 0.003, odds ratio (OR) 1.049, 95% confidence interval (CI) 1.016–1.082] after adjustment for hematoma volume. The best cutoff point of iPHE volume was 7.98 mL with a specificity of 71.4% and a sensitivity of 47.5%. Diabetes mellitus (P = 0.043, β = 7.66 95% CI 0.26–15.07), black hole sign (P = 0.002, β = 18.93 95% CI 6.84–31.02), and initial ICH volume (P = 0.018, β = 0.20 95% CI 0.03–0.37) were significantly associated with iPHE volume. After adjusting for hematoma expansion, the black hole sign could still independently predict the increase of PHE (P < 0.001, β = 21.62 95% CI 10.10–33.15).

Conclusions: An increase of PHE volume >7.98 mL from baseline to day 3 may lead to poor outcome. Patients with diabetes mellitus, black hole sign, and large initial hematoma volume result in more PHE growth, which should garner attention in the treatment.
Effects of Brain Atlases and Machine Learning Methods on the Discrimination of Schizophrenia Patients: A Multimodal MRI Study
Authors: Zang Jinyu , Huang Yuanyuan , Kong Lingyin , Lei Bingye , Ke Pengfei , Li Hehua , Zhou Jing , Xiong Dongsheng , Li Guixiang , Chen Jun , Li Xiaobo , Xiang Zhiming , Ning Yuping , Wu Fengchun , Wu Kai
Published: July 2021

Recently, machine learning techniques have been widely applied in discriminative studies of schizophrenia (SZ) patients with multimodal magnetic resonance imaging (MRI); however, the effects of brain atlases and machine learning methods remain largely unknown. In this study, we collected MRI data for 61 first-episode SZ patients (FESZ), 79 chronic SZ patients (CSZ) and 205 normal controls (NC) and calculated 4 MRI measurements, including regional gray matter volume (GMV), regional homogeneity (ReHo), amplitude of low-frequency fluctuation and degree centrality. We systematically analyzed the performance of two classifications (SZ vs NC; FESZ vs CSZ) based on the combinations of three brain atlases, five classifiers, two cross validation methods and 3 dimensionality reduction algorithms. Our results showed that the groupwise whole-brain atlas with 268 ROIs outperformed the other two brain atlases. In addition, the leave-one-out cross validation was the best cross validation method to select the best hyperparameter set, but the classification performances by different classifiers and dimensionality reduction algorithms were quite similar. Importantly, the contributions of input features to both classifications were higher with the GMV and ReHo features of brain regions in the prefrontal and temporal gyri. Furthermore, an ensemble learning method was performed to establish an integrated model, in which classification performance was improved. Taken together, these findings indicated the effects of these factors in constructing effective classifiers for psychiatric diseases and showed that the integrated model has the potential to improve the clinical diagnosis and treatment evaluation of SZ.
CCL2: An Important Mediator Between Tumor Cells and Host Cells in Tumor Microenvironment
Authors: Jin Jiakang , Lin Jinti , Xu Ankai , Lou Jianan , Qian Chao , Li Xiumao , Wang Yitian , Yu Wei , Tao Huimin
Published: July 2021

Tumor microenvironment (TME) formation is a major cause of immunosuppression. The TME consists of a considerable number of macrophages and stromal cells that have been identified in multiple tumor types. CCL2 is the strongest chemoattractant involved in macrophage recruitment and a powerful initiator of inflammation. Evidence indicates that CCL2 can attract other host cells in the TME and direct their differentiation in cooperation with other cytokines. Overall, CCL2 has an unfavorable effect on prognosis in tumor patients because of the accumulation of immunosuppressive cell subtypes. However, there is also evidence demonstrating that CCL2 enhances the anti-tumor capability of specific cell types such as inflammatory monocytes and neutrophils. The inflammation state of the tumor seems to have a bi-lateral role in tumor progression. Here, we review works focusing on the interactions between cancer cells and host cells, and on the biological role of CCL2 in these processes.
Three-Method Hybrid Numerical Simulation for Surface-Plasmon-Enhanced GaInN-Based Light-Emitting Diodes with Metal-Embedded Nanostructures
Authors: Hiramatsu Ryoya , Takahashi Ryo , Fujiki Ryoto , Hozo Keisuke , Sawai Kanato , Han Dong-Pyo , Iwaya Motoaki , Takeuchi Tetsuya , Kamiyama Satoshi , Akasaki Isamu
Published: July 2021

In this paper, a hybrid numerical simulation tool is introduced and performed for GaInN-based light-emitting diodes (LEDs) with metal-embedded nanostructure to theoretically predict external quantum efficiency (EQE), which composed of finite-difference time-domain, rigorous coupled wave analysis, and ray tracing. The advantage is that the proposed method provides results supported by sufficient physical background within a reasonable calculation time. From the simulation results, the EQE of LED with Ag-nanoparticles embedded nanostructure is expected to be enhanced by as high as ∼1.6 times the conventional LED device in theory.
The Evolving Role of Next-Generation Sequencing in Screening and Diagnosis of Hemoglobinopathies
Authors: Achour Ahlem , Koopmann Tamara T. , Baas Frank , Harteveld Cornelis L.
Published: July 2021

During the last few years, next-generation sequencing (NGS) has undergone a rapid transition from a research setting to a clinical application, becoming the method of choice in many clinical genetics laboratories for the detection of disease-causing variants in a variety of genetic diseases involving multiple genes. The hemoglobinopathies are the most frequently found Mendelian inherited monogenic disease worldwide and are composed of a complex group of disorders frequently involving the inheritance of more than one abnormal gene. This review aims to present the role of NGS in both screening and pre- and post-natal diagnostics of the hemoglobinopathies, and the added value of NGS is discussed based on the results described in the literature. Overall, NGS has an added value in large-scale high throughput carrier screening and in the complex cases for which common molecular techniques have some inadequacies. It is proven that the majority of thalassemia cases and Hb variants can be diagnosed using routine analysis involving a combined approach of hematology, hemoglobin separation, and classical DNA methods; however, we conclude that NGS can be a useful addition to the existing methods in the diagnosis of these disorders.
Molecular Basis of Taste and Micronutrient Content in Kumamoto Oysters (Crassostrea Sikamea) and Portuguese Oysters (Crassostrea Angulata) From Xiangshan Bay
Authors: Liu Sheng , Xu Hongqiang , Jian Shoushuo , Xue Qinggang , Lin Zhihua
Published: July 2021

Oysters are the most extensively cultivated bivalves globally. Kumamoto oysters, which are sympatric with Portuguese oysters in Xiangshan bay, China, are regarded as particularly tasty. However, the molecular basis of their characteristic taste has not been identified yet. In the present study, the taste and micronutrient content of the two oyster species were compared. Portuguese oysters were larger and had a greater proportion of proteins (48.2 ± 1.6%), but Kumamoto oysters contained significantly more glycogen (21.8 ± 2.1%; p < 0.05). Moisture and lipid content did not differ significantly between the two species (p > 0.05). Kumamoto oysters contained more Ca, Cu, and Zn (p < 0.05); whereas Mg and Fe levels were comparable (p > 0.05). Similarly, there was no significant difference between the two species with respect to total amount of free amino acids, umami and bitterness amino acids, succinic acid (SA), and most flavoring nucleotides (p > 0.05). In contrast, sweetness amino acids were significantly more abundant in Portuguese oysters. Volatile organic compounds profiles of the two species revealed a higher proportion of most aldehydes including (2E,4E)-hepta-2,4-dienal in Kumamoto oysters. Overall, Kumamoto oysters contain abundant glycogen, Ca, Zn, and Cu, as well as volatile organic compounds, especially aldehydes, which may contribute to their special taste. However, free amino acid and flavor nucleotides may not the source of special taste of Kumamoto oyster. These results provide the molecular basis for understanding the characteristic taste of Kumamoto oysters and for utilizing local oyster germplasm resources.
LED Intercanopy Lighting in Blackberry During Spring Improves Yield as a Result of Increased Number of Fruiting Laterals and Has a Positive Carryover Effect on Autumn Yield
Authors: Rivas Anabel , Liu Kang , Heuvelink Ep
Published: July 2021

High market price and low availability of local winter and spring production has stimulated production of blackberries in glasshouses at northern latitudes. For this production, light is the main limiting factor. We investigated the potential of intercanopy lighting (ICL) using light emitting diodes (LEDs) to improve blackberry fruit yield in a crop with a spring and an autumn production cycle. During the spring production cycle three light treatments were applied: only natural light (no ICL), 93 or 185 μmol m–2s–1ICL In summer the lateral shoots were cut back and 93 μmol m–2s–1ICL was applied to all plants after cutting back, investigating a possible carryover effect of supplemental light in spring on autumn production. Fresh fruit yield in spring increased by 79 and 122% with 93 and 185 μmol m–2s–1ICL, respectively, compared to no ICL. This represents 3.6 and 2.8% increase in harvestable product for every additional 1% of light. A yield component analysis and leaf photosynthesis measurements were conducted. Maximum photosynthetic capacity (Amax) for leaves at 185 μmol m–2s–1ICL was about 50% higher, and LAI was 41% higher compared to no ICL. ICL increased the number of fruiting laterals per cane, and this explained 75% of the increase in yield. ICL at 185 μmol m–2s–1resulted in a higher yield compared to no ICL, primarily as a result of higher total dry matter production. Furthermore, a higher fraction of dry matter partitioned to the fruits (0.59 compared to 0.52) contributed to yield increase, whereas fruit dry matter content and fruit quality (sugar and acid content) was not affected by ICL. Averaged over the three light treatments autumn yield was 47% lower than spring yield. Autumn yield was 10% higher for plants at ICL 93 μmol m–2s–1in spring and 36% higher for plants at 185 μmol m–2s–1in spring compared to no ICL in spring. This increased autumn yield was caused by more fruiting laterals (less necrotic buds). It is concluded that management practices in spring can have a carryover effect on the autumn production. This is the first scientific paper on the potential for applying LED ICL in blackberries. Further research should focus on optimal intensity of ICL, positioning of supplementary lighting and economic feasibility.
Development of Submergence-Tolerant, Bacterial Blight-Resistant, and High-Yielding Near Isogenic Lines of Popular Variety, ‘Swarna’ Through Marker-Assisted Breeding Approach
Authors: Mohapatra Shibani , Panda Alok Kumar , Bastia Akshaya Kumar , Mukherjee Arup Kumar , Sanghamitra Priyadarshini , Meher Jitendriya , Mohanty Shakti Prakash , Pradhan Sharat Kumar
Published: July 2021

The rice variety ‘Swarna’ is highly popular in the eastern region of India. The farmers of eastern India cultivate mainly rainfed rice and face the adverse effects of climate change very frequently. Rice production in this region is not stable. Swarna variety is highly susceptible to bacterial blight (BB) disease and flash floods, which cause a heavy reduction in the yield. Transfer of five target genes/QTLs was targeted into the variety, Swarna by adopting marker-assisted backcross breeding approach. Direct markers for Sub1A, OsSPL14, and SCM2 QTLs and closely linked markers for xa5 and Xa21 BB resistance genes were screened in the backcross progenies. Swarna-Sub1, CR Dhan 800, and Swarna–Habataki near-isogenic lines (NILs) were used as donor parents in the breeding program. True multiple F1 plants were identified for backcrossing, and 796 BC1F1 seeds were generated. Foreground selection detected all the five target genes in six progenies in BC1F1 generation. The progeny containing all the target genes and more similar with the recipient parent was backcrossed, and a total of 446 BC2F1 seeds were produced. Foreground screening detected four BC2F1 plants carrying the five target genes. A total of 2,145 BC2F2 seeds were obtained from the best BC2F1 progeny. Screening of the progenies resulted in one plant with all five desirable genes, three plants with four, and another three progenies carrying three genes in homozygous conditions. The pyramided lines showed higher BB resistance and submergence tolerance than the recipient parent, Swarna. Culm strength of the pyramided lines showed higher breaking force than the recipient parent, Swarna. The pyramided line, SSBY-16-68-69 yielded the highest grain yield of 7.52 t/ha followed by the lines SSBY-16-68-511 (7.34 t/ha) and SSBY-16-68-1633 (7.02 t/ha). The best-pyramided line showed a yield advantage of 18% over the recipient parent and 6.8% over the yield component donor parent. Seven pyramided lines showed higher yield than the recipient parent, while five lines were better yielders than the yield component donor parent. The pyramided line SSBY-16-68-69 produced 365 grains/panicle, while the recipient had 152. The main morphologic and grain quality features of the recipient parent were retained in the pyramided lines.
The C-Terminal Half of SARS-CoV-2 Nucleocapsid Protein, Industrially Produced in Plants, Is Valid as Antigen in COVID-19 Serological Tests
Authors: Williams Laura , Jurado Silvia , Llorente Francisco , Romualdo Alberto , González Sara , Saconne Ana , Bronchalo Isabel , Martínez-Cortes Mercedes , Pérez-Gómez Beatriz , Ponz Fernando , Jiménez-Clavero Miguel Ángel , Lunello Pablo
Published: July 2021

Background

The fight against the current coronavirus disease 2019 (COVID-19) pandemic has created a huge demand of biotechnological, pharmaceutical, research and sanitary materials at unprecedented scales. One of the most urgent demands affects the diagnostic tests. The growing need for rapid and accurate laboratory diagnostic tests requires the development of biotechnological processes aimed at producing reagents able to cope with this demand in a scalable, cost-effective manner, with rapid turnaround times. This is particularly applicable to the antigens employed in serological tests. Recombinant protein expression using plants as biofactories is particularly suitable for mass production of protein antigens useful in serological diagnosis, with a neat advantage in economic terms.

Methods

We expressed a large portion of the nucleoprotein (N) derived from SARS-CoV-2 in Nicotiana benthamiana plants. After purification, the recombinant N protein obtained was used to develop an indirect enzyme-linked immunosorbent assay (ELISA) for detection of antibodies to SARS-CoV-2 in human sera. To validate the ELISA, a panel of 416 sera from exposed personnel at essential services in Madrid City Council were tested, and the results compared to those obtained by another ELISA, already validated, used as reference. Furthermore, a subset of samples for which RT-PCR results were available were used to confirm sensitivity and specificity of the test.

Results

The performance of the N protein expressed in plants as antigen in serologic test for SARS-CoV-2 antibody detection was shown to be highly satisfactory, with calculated diagnostic sensitivity of 96.41% (95% CI: 93.05–98.44) and diagnostic specificity of 96.37 (95% CI: 93.05–98.44) as compared to the reference ELISA, with a kappa (K) value of 0.928 (95% CI:0.892–0.964). Furthermore, the ELISA developed with plant-derived N antigen detected SARS-CoV-2 antibodies in 84 out of 93 sera from individuals showing RT-PCR positive results (86/93 for the reference ELISA).

Conclusion

This study demonstrates that the N protein part derived from SARS-CoV-2 expressed in plants performs as a perfectly valid antigen for use in COVID-19 diagnosis. Furthermore, our results support the use of this plant platform for expression of recombinant proteins as reagents for COVID-19 diagnosis. This platform stands out as a convenient and advantageous production system, fit-for-purpose to cope with the current demand of this type of biologicals in a cost-effective manner, making diagnostic kits more affordable.
Development of Preschoolers’ Understanding of Zero
Authors: Krajcsi Attila , Kojouharova Petia , Lengyel Gábor
Published: July 2021

While knowledge on the development of understanding positive integers is rapidly growing, the development of understanding zero remains not well-understood. Here, we test several components of preschoolers’ understanding of zero: Whether they can use empty sets in numerical tasks (as measured with comparison, addition, and subtraction tasks); whether they can use empty sets soon after they understand the cardinality principle (cardinality-principle knowledge is measured with the give-N task); whether they know what the word “zero” refers to (tested in all tasks in this study); and whether they categorize zero as a number (as measured with the smallest-number and is-it-a-number tasks). The results show that preschoolers can handle empty sets in numerical tasks as soon as they can handle positive numbers and as soon as, or even earlier than, they understand the cardinality principle. Some also know that these sets are labeled as “zero.” However, preschoolers are unsure whether zero is a number. These results identify three components of knowledge about zero: operational knowledge, linguistic knowledge, and meta-knowledge. To account for these results, we propose that preschoolers may understand numbers as the properties of items or objects in a set. In this view, zero is not regarded as a number because an empty set does not include any items, and missing items cannot have any properties, therefore, they cannot have the number property either. This model can explain why zero is handled correctly in numerical tasks even though it is not regarded as a number.
A Case for Cognitive Entrenchment: To Achieve Optimal Best, Taking Into Account the Importance of Perceived Optimal Efficiency and Cognitive Load Imposition
Authors: Phan Huy P. , Ngu Bing Hiong
Published: July 2021

One interesting observation that we may all concur with is that many experts, or those who are extremely knowledgeable and well-versed in their respective domains of functioning, become “mediocre” and lose their “touch of invincibility” over time. For example, in the world of professional football, it has been argued that an elite football coach would lose his/her air of invincibility and demise after 10–15 years at the top. Why is this the case? There are different reasons and contrasting viewpoints that have been offered to account for this observed demise. One notable concept, recently introduced to explain this decline, is known ascognitive entrenchment, which is concerned with a high level of stability in one's domain schemas (Dane,2010). This entrenchment or “situated fixation,” from our proposition, may act to deter the flexibility and/or willingness of a person to adapt to a new context or situation. Some writers, on this basis, have argued that cognitive entrenchment would help explain the demise of some experts and/or why some students have difficulties adapting to new situations. An initial inspection would seem to indicate that cognitive entrenchment is detrimental, potentially imparting evidence of inflexibility, difficulty, and/or the unwillingness of a person to adapt to new contexts (Dane,2010). This premise importantly connotes that expertise may constrain a person from being flexible, innovative, and/or creative to ongoing changes. In this analysis, an expert may experience a cognitive state of entrenchment, facilitated in this case by his/her own experience, knowledge, and/or theoretical understanding of a subject matter. Having said this, however, it is also a plausibility that cognitive entrenchment in itself espouses some form of positivity, giving rise to improvement and/or achievement of different types of adaptive outcomes. Drawing from our existing research development, we propose in this conceptual analysis article that personal “entrenchment” to a particular context (e.g., the situated fixation of a football coach to a particular training methodology) may closely relate to three major elements:self-cognizance of cognitive load imposition, aneed for efficiency, and thequest for stability and comfort. As we explore later, there is credence to accept the “positivity” of cognitive entrenchment—that by nature, for example, a person would purposively choose thestatus quoin order to minimize cognitive load imposition, optimize efficiency, and/or to achieve minimum disruption and a high level of comfort, which could then “optimize” his/her learning experiences. We strongly believe that our propositions, which consider eight in this article, are of significance and may, importantly, provide grounding for further research development into the validity of cognitive entrenchment.
Real-World Robot Evolution: Why Would it (not) Work?
Authors: Eiben A.E.
Published: July 2021

This paper takes a critical look at the concept of real-world robot evolution discussing specific challenges for making it practicable. After a brief review of the state of the art several enablers are discussed in detail. It is noted that sample efficient evolution is one of the key prerequisites and there are various promising directions towards this in different stages of maturity, including learning as part of the evolutionary system, genotype filtering, and hybridizing real-world evolution with simulations in a new way. Furthermore, it is emphasized that an evolutionary system that works in the real world needs robots that work in the real world. Obvious as it may seem, to achieve this significant complexification of the robots and their tasks is needed compared to the current practice. Finally, the importance of not only building but also understanding evolving robot systems is emphasised, stating that in order to have the technology work we also need the science behind it.
Allogeneic Stem Cell Transplantation for Relapsed and Refractory Hodgkin Lymphoma: Real World Experience of a Single Center
Authors: Kopińska A. , Koclęga A. , Wieczorkiewicz-Kabut A. , Woźniczka K. , Kata D. , Włodarczyk M. , Helbig G.
Published: July 2021

Introduction: Refractory and relapsed Hodgkin lymphoma (R/R HL) is associated with poor prognosis, and allogeneic stem cell transplantation (allo-SCT) remains the only potentially curative approach.

Aim: The aim of the study was to evaluate the feasibility of allotransplantation in R/R HL setting.

Material: Overall, 24 patients (17 men and 7 women) at a median age of 27 years (range 18–44) underwent allo-SCT between 2002 and 2020.

Results: Nineteen patients received prior autologous stem cell transplantation (ASCT1) whereas eight patients received second ASCT (ASCT2) after failure of ASCT1. Six patients received only brentuximab vedotin (BV; n = 4) or BV followed by checkpoint inhibitors (CPI; n = 2) before entering allo-SCT. Median time from ASCT1 to allo-SCT was 17.1 months. Fifteen patients received grafts from unrelated donors. Peripheral blood was a source of stem cells for 16 patients. Reduced-intensity conditioning was used for all patients. Disease status at transplant entry was as follows: complete remission (CR; n = 4), partial response (PR; n = 10), and stable disease (SD; n = 10). Acute and chronic graft-versus-host disease (GVHD) developed in 13 (54%) and 4 (16%) patients, respectively. Median follow-up for the entire cohort was 13.3 months. At the last follow-up, 17 (71%) patients died. The main causes of death were disease progression (n = 10), infectious complications (n = 6), and steroid-resistant GVHD (n = 1). Non-relapse mortality at 12 months was 25%. At the last follow-up, seven patients were alive; six patients were in CR, and one had PR. The 2-year overall survival (OS) was 40%.

Conclusion: Chemosensitive disease at transplant was associated with better outcome. Allo-SCT allows for long-term survival in refractory and relapsed HL.
Organic Amendment vs. Mineral Fertilization under Minimum Tillage: Changes in Soil Nutrients, Soil Organic Matter, Biological Properties and Yield after 10 Years
Authors: Martín-Lammerding Diana , Gabriel José L. , Zambrana Encarnación , Santín-Montanyá Inés , Tenorio José L.
Published: July 2021

Conservation tillage is recognized as a sustainable management practice, however its combination with organic residues application still constitutes a challenge in some areas. A field trial was established in a semiarid agro-ecosystem to study the effects of different crop nutrient sources under minimum tillage (MT). Application of organic amendments at the beginning of a five-year crop rotation (ORG treatment) was evaluated and compared to the control plot where wheat was continuously grown with traditional mineral fertilization (MIN treatment). In addition to wheat yield and biomass, several soil properties were measured (pH, soil nutrients (i.e., Olsen P, exchangeable K, and mineral N), soil organic matter (i.e., C, N, C/N ratio), potentially mineralizable nitrogen, total microbial activity and heavy metals) throughout the 10-year study. The wheat yield was significantly higher under the ORG treatment than under the MIN, although climatic conditions (e.g., rainfall) exerted a great influence too. The organic amendments increased soil nutrients content mainly right after their application but the levels were adequate for the whole crop rotation. Plots under organic amendment application did not accumulate significantly more soil organic matter than those mineral-fertilized, probably due to the low protective capacity of coarse-textured soils. The application of organic amendments under MT resulted in a promising management agro-ecosystem compared to the mineral fertilized because crop nutrients came from organic wastes, no herbicides were applied while the yields were higher.
An Italian Explorative Study of Willingness to Pay for a New Functional Pasta Featuring Opuntia ficus indica
Authors: Palmieri Nadia , Stefanoni Walter , Latterini Francesco , Pari Luigi
Published: July 2021

Opuntia ficus indica is a source of vitamins and minerals, and recently, it has been used as ingredient to make a new functional pasta. Italy produces the highest amount of pasta in the world and it is also the second-highest producer of opuntia globally. This study sought to identify the consumer willingness to pay for pasta featuring Opuntia ficus indica and to understand what factors influence their choice. This study is the first in food research to analyze consumers’ willingness to pay for a new functional pasta featuring Opuntia ficus indica. Data were collected by using a web-based survey; the sample was composed of 342 consumers and an ordered logistic regression model was applied. The findings showed that the educational and higher income levels of respondents play important roles in the willingness to pay for functional pasta. Moreover, the healthy and nutritional features of functional pasta together with the respondents’ willingness to eat it, their curiosity towards this new food and their need to get more information about functional pasta should improve consumers’ willingness to pay for it. The findings should be useful for firms to draw marketing strategies considering the positive consumers’ attitude toward the use of Opuntia ficus indica in pasta production.
Optimizing Nitrogen Application in Root Vegetables from Their Growth, Biochemical and Antioxidant Response to Urea Fertilizer
Authors: Shakeel Adnan , Khan Abrar Ahmad , Alharby Hesham F. , Bamagoos Atif A. , Alabdallah Nadiyah M. , Hakeem Khalid Rehman
Published: July 2021

Nitrogen is one of the most influencing inorganic nutrients for improved plant growth and yield in crops. However, excessive fertilizer application may have adverse impacts on the environment. Therefore, we strive to investigate in this work by examining the impact of different nitrogen (N) doses in the form of urea (46% N) on the growth, yield, photosynthetic pigment content, nitrate reductase activity, carbohydrate content, protein content, and antioxidant enzyme activity of the carrot and beetroot. A pot experiment was conducted under natural conditions with four nitrogen levels as basal treatment (Control = Nil N, U50 = 145.57 mg/kg N; U100 = 291.14 mg/kg N; U150 = 436.71 mg/kg N; U200 = 582.28 mg/kg N). Results found that U150 (436.71 mg/kg N) is the optimum N fertilizer dose at which significant (p ≤ 0.05) improvements in all the growth, yield and biochemical attributes of carrot and beetroot were observed. However, the further increment in N doses did not affect the observed parameters and, therefore, excessive N level was observed beyond U150 = 436.71 mg/kg N. The principal component analysis presented significant correlations among the various parameters observed. Two principal components account for a total of 98.86% variance (PC1 =  92.96%; PC2 =  5.90%) in carrot and 99.2% variance (PC1 = 92.64; PC = 6.56) in beetroot of the overall data variability in plants supplemented with different N treatments.
Hydroponic Lettuce Cultivation Using Organic Nutrient Solution from Aerobic Digested Aquacultural Sludge
Authors: Ezziddine Maha , Liltved Helge , Seljåsen Randi
Published: July 2021

The aim of this study was to demonstrate how aquacultural sludge can be processed and utilized as an organic nutrient solution (ONS) for hydroponic lettuce production. By using a previous developed method, approximately 80% of the processed sludge was reclaimed as a clear, nutrient-rich solution. The performance of the recovered nutrient solution on lettuce growth was assessed in a nutrient film hydroponic system. The results were compared to the results obtained using a conventional nutrient solution (CNS). Yield, fresh weight, water consumption, and nutrient and heavy metal content in leaf tissue were measured. In spite of a 16% lower average fresh weight obtained in ONS compared to the weight obtained in CNS, there was no statistical difference of the yield of lettuce among the two nutrient solutions. After the cultivation period, 90% of the lettuce heads grown in ONS exceeded the marked weight of 150 g. Foliar analysis revealed a similar or higher content of all nutrients, except of magnesium and molybdenum in the leaves of lettuce grown in the ONS compared to lettuce grown in the CNS. This study shows that nutrients recovered from aquacultural sludge can be utilized as fertilizer, thereby reducing the dependency on mineral fertilizer in hydroponic and aquaponic systems.