bluuebunny/crossref_metadata_2025 huggingface dataset:
Knockdown of tgfb1a partially improves ALS phenotype in a transient zebrafish model
Authors: Gonzalez David , Cuenca Xiomara , Allende Miguel L.
Published: April 2024

Amyotrophic lateral sclerosis (ALS) corresponds to a neurodegenerative disorder marked by the progressive degeneration of both upper and lower motor neurons located in the brain, brainstem, and spinal cord. ALS can be broadly categorized into two main types: sporadic ALS (sALS), which constitutes approximately 90% of all cases, and familial ALS (fALS), which represents the remaining 10% of cases. Transforming growth factor type-β (TGF-β) is a cytokine involved in various cellular processes and pathological contexts, including inflammation and fibrosis. Elevated levels of TGF-β have been observed in the plasma and cerebrospinal fluid (CSF) of both ALS patients and mouse models. In this perspective, we explore the impact of the TGF-β signaling pathway using a transient zebrafish model for ALS. Our findings reveal that the knockdown of tgfb1a lead to a partial prevention of motor axon abnormalities and locomotor deficits in a transient ALS zebrafish model at 48 h post-fertilization (hpf). In this context, we delve into the proposed distinct roles of TGF-β in the progression of ALS. Indeed, some evidence suggests a dual role for TGF-β in ALS progression. Initially, it seems to exert a neuroprotective effect in the early stages, but paradoxically, it may contribute to disease progression in later stages. Consequently, we suggest that the TGF-β signaling pathway emerges as an attractive therapeutic target for treating ALS. Nevertheless, further research is crucial to comprehensively understand the nuanced role of TGF-β in the pathological context.
Altered serum cytokines in patients with symptomatic disk herniation and depressive symptoms
Authors: Bielewicz Joanna , Daniluk Beata , Kamieniak Piotr
Published: April 2024

Purpose

An increasing number of studies have indicated the important role of cytokines in the development of depressive disturbances (DD). In medically ill patients, cytokines can provoked sickness behavior, the signs of which resemble DD. This results in alterations in behavior to limit energy expenditure and redirect it to cope with particular diseases. The aim of our study was to investigate the role of pro-inflammatory IL-6, TNF-α, and IL-1β and anti-inflammatory IL-10 and TGF-β in DD observed in patients suffering from pain caused by disk herniation (DH) qualified for surgery.

Patients and methods

The intensity of DD assessed by using Beck Depression Inventory, pain intensity, and functional impairment were evaluated in 70 patients with DH who were qualified for surgery. Pro-inflammatory serum levels of TNF-α, IL-1, IL-6, anti-inflammatory TGF-β, and IL-10 were measured.

Results

Elevated serum levels of TGF-β, IL-10, and IL-6 were found in the group with moderate and severe depressive symptoms (SD) compared with the groups with mild (MD) or no depressive symptoms (ND). TGF-β levels were negatively correlated with pain intensity, as assessed using the Present Pain Intensity scale in SD. Functional impairment measured using the Oswestry Disability Index was the most advanced in SD group.

Conclusion

Results of our study can suggest association between depressive disturbances and anti-inflammatory cytokines TGF-β and IL-10. Functional impairment of SD group is more severe but serum levels of TGF-β and IL-10, which are involved in the healing processes, are increased.
ESF-YOLO: an accurate and universal object detector based on neural networks
Authors: Tao Wenguang , Wang Xiaotian , Yan Tian , Liu Zhengzhuo , Wan Shizheng
Published: April 2024

As an excellent single-stage object detector based on neural networks, YOLOv5 has found extensive applications in the industrial domain; however, it still exhibits certain design limitations. To address these issues, this paper proposes Efficient Scale Fusion YOLO (ESF-YOLO). Firstly, the Multi-Sampling Conv Module (MSCM) is designed, which enhances the backbone network’s learning capability for low-level features through multi-scale receptive fields and cross-scale feature fusion. Secondly, to tackle occlusion issues, a new Block-wise Channel Attention Module (BCAM) is designed, assigning greater weights to channels corresponding to critical information. Next, a lightweight Decoupled Head (LD-Head) is devised. Additionally, the loss function is redesigned to address asynchrony between labels and confidences, alleviating the imbalance between positive and negative samples during the neural network training. Finally, an adaptive scale factor for Intersection over Union (IoU) calculation is innovatively proposed, adjusting bounding box sizes adaptively to accommodate targets of different sizes in the dataset. Experimental results on the SODA10M and CBIA8K datasets demonstrate that ESF-YOLO increases Average Precision at 0.50 IoU (AP50) by 3.93 and 2.24%, Average Precision at 0.75 IoU (AP75) by 4.77 and 4.85%, and mean Average Precision (mAP) by 4 and 5.39%, respectively, validating the model’s broad applicability.
Bioelectronic modulation of carotid sinus nerve to treat type 2 diabetes: current knowledge and future perspectives
Authors: Conde Silvia V. , Sacramento Joana F. , Zinno Ciro , Mazzoni Alberto , Micera Silvestro , Guarino Maria P.
Published: April 2024

Bioelectronic medicine are an emerging class of treatments aiming to modulate body nervous activity to correct pathological conditions and restore health. Recently, it was shown that the high frequency electrical neuromodulation of the carotid sinus nerve (CSN), a small branch of the glossopharyngeal nerve that connects the carotid body (CB) to the brain, restores metabolic function in type 2 diabetes (T2D) animal models highlighting its potential as a new therapeutic modality to treat metabolic diseases in humans. In this manuscript, we review the current knowledge supporting the use of neuromodulation of the CSN to treat T2D and discuss the future perspectives for its clinical application. Firstly, we review in a concise manner the role of CB chemoreceptors and of CSN in the pathogenesis of metabolic diseases. Secondly, we describe the findings supporting the potential therapeutic use of the neuromodulation of CSN to treat T2D, as well as the feasibility and reversibility of this approach. A third section is devoted to point up the advances in the neural decoding of CSN activity, in particular in metabolic disease states, that will allow the development of closed-loop approaches to deliver personalized and adjustable treatments with minimal side effects. And finally, we discuss the findings supporting the assessment of CB activity in metabolic disease patients to screen the individuals that will benefit therapeutically from this bioelectronic approach in the future.
Allelic heterogeneity and abnormal vesicle recycling in PLAA-related neurodevelopmental disorders
Authors: Iacomino Michele , Houerbi Nadia , Fortuna Sara , Howe Jennifer , Li Shan , Scorrano Giovanna , Riva Antonella , Cheng Kai-Wen , Steiman Mandy , Peltekova Iskra , Yusuf Afiqah , Baldassari Simona , Tamburro Serena , Scudieri Paolo , Musante Ilaria , Di Ludovico Armando , Guerrisi Sara , Balagura Ganna
Published: April 2024

The human PLAA gene encodes Phospholipase-A2-Activating-Protein (PLAA) involved in trafficking of membrane proteins. Through its PUL domain (PLAP, Ufd3p, and Lub1p), PLAA interacts with p97/VCP modulating synaptic vesicles recycling. Although few families carrying biallelic PLAA variants were reported with progressive neurodegeneration, consequences of monoallelic PLAA variants have not been elucidated. Using exome or genome sequencing we identified PLAA de-novo missense variants, affecting conserved residues within the PUL domain, in children affected with neurodevelopmental disorders (NDDs), including psychomotor regression, intellectual disability (ID) and autism spectrum disorders (ASDs). Computational and in-vitro studies of the identified variants revealed abnormal chain arrangements at C-terminal and reduced PLAA-p97/VCP interaction, respectively. These findings expand both allelic and phenotypic heterogeneity associated to PLAA-related neurological disorders, highlighting perturbed vesicle recycling as a potential disease mechanism in NDDs due to genetic defects of PLAA.
The effect of Internet use on nutritional intake and health outcomes: new evidence from rural China
Authors: Deng Zhilong , Liu Jian , Hong Yu , Liu Weigang
Published: April 2024

Introduction

Internet use is changing nutritional intake and health outcomes, but the results are mixed, and less attention is given to the rural developing regions. Based on the China Health and Nutrition Survey (CHNS) data from 2004 to 2015, this study seeks to better understand the effect of Internet use on nutritional intake and health outcomes.

Methods

An instrumental variable estimation is used to address endogeneity problem.

Results

The results show that Internet use improves the dietary knowledge of rural residents, and thus has a positive impact on dietary quality, such as healthy eating index (HEI) and dietary diversity score (DDS). The higher the dietary quality, the better the nutritional health status. However, results also show that Internet use increases the risk of overweight, and obesity among rural Chinese residents. Because Internet use has significantly reduced the physical activity of rural residents in China. Interestingly, we also find that the Internet increases the risk of chronic diseases such as diabetes and high blood pressure, but there is a positive causal relationship between Internet use and the self-assessment score of health.

Discussion

Our findings suggest that there may be a serious lack of awareness of the health risks of chronic diseases among Chinese rural residents. Therefore, policymakers are suggested to consider the possible negative effects when promoting digital development.
Transarterial chemoembolization combined with radiofrequency ablation for medium and large hepatocellular carcinoma: insufficient ablation is associated with intrahepatic distant metastasis and extrahepatic metastasis
Authors: Guo Peng , Zheng Junjun , Pi Xingtao , Gao Feng , Zhao Yushan , Xie Chunming , Cao Wendong
Published: April 2024

Purpose

To compare the prognosis of complete and insufficient ablation of transarterial chemoembolization (TACE) combined with radiofrequency ablation (RFA) in treating medium and large hepatocellular carcinoma (HCC) and to explore the differences in recurrence patterns between the two groups

Patients and methods

Patients´ medical records and imaging data of patients with confirmed HCC from January 2014 to January 2022 were collected. These patients were divided into 2 groups: complete ablation (n=172) and insufficient ablation (n=171). Overall survival (OS) and progression-free survival (PFS) were estimated by the Kaplan-Meier curve and the log-rank test was used to compared. Fisher’s exact test was used to compare recurrence patterns between the two groups.

Results

The median OS time was 72.8 months (95%CI:69.5-76.1) and 62.0 months (95%CI: 55.3-68.7) in the complete and insufficient ablation groups, respectively. The median PFS time in the complete ablation group was 67.8 months (95% CI: 65.2-70.4) and 38.6 months (95%CI: 29.8-47.4) in the insufficient ablation group. The OS and PFS rates of the complete ablation group were significantly better than those of the insufficient ablation group (P<0.001). In the complete ablation group, 25(41%) patients experienced local tumor progression(LTP), 36(59%) experienced intrahepatic distant progression(IDP), and 0(0%) experienced extrahepatic progression (EP). In the insufficient ablation group, 51 (32.1%) patients experienced LTP, 96 (60.4%) experienced IDP, and 12 (7.5%) experienced EP. The progression patterns of the two groups were statistically significant (P=0.039).

Conclusion

Insufficient ablation indicates a poor survival outcome of TACE combined with RFA for medium and large HCC and can promote intrahepatic distant and extrahepatic metastasis.
TACE-assisted multi-image guided radiofrequency ablation for the treatment of single hepatocellular carcinoma ≤ 5 cm: a retrospective study
Authors: Xie Yong , Lyu Tianshi , Song Li , Tong Xiaoqiang , Wang Jian , Zou Yinghua
Published: April 2024

Background/Objective

Treatment of hepatocellular carcinoma (HCC) with ablation alone often results in high rates of recurrence and metastasis, reaching up to 25.9% within two years. Therefore, this study aimed to examine the efficacy and safety of transarterial chemoembolization (TACE)-assisted multi-image guided radiofrequency ablation (RFA) for the treatment of stage Ia HCC according to the China liver cancer staging (CNLC).

Methods

This study enrolled and analyzed a total of 118 patients diagnosed with HCC, each with a single nodular lesion no larger than 5 cm, who received TACE-RFA as first-line therapy between February 1, 2014, and December 31, 2021. The median/mean follow-up period was 29.0 months [95% confidence interval (CI): 21.8-36.2 months] and 31.8 months (95% CI: 27.5-36.0 months), respectively. We assessed the treatment’s effectiveness, potential complications, and survival rate.

Results

The technical success rate was 100% (118/118) after the initial treatment. Out of the total, 3 out of 118 patients (2.5%) developed local tumor progression (LTP) during the follow-up period. The median time for LTP was 29.0 months (95%CI: 21.9-36.1 months; mean: 31.5 months; range 1-92 months). At 1, 3, 5, and 7 years after treatment, the cumulative LTP rates were 0%, 4.6%, 4.6%, and 4.6%, respectively. The overall survival rates at 1, 3, 5, and 7 years were 100%, 95.2%, 95.2%, and 95.2%, respectively. In total, 28 patients experienced minor Grade B complications, and no major complications or treatment-related mortality occurred.

Conclusion

The treatment of CNLC stage Ia HCC using TACE-assisted multi-image-guided RFA was found to be both safe and feasible.
Navigating cholestasis: identifying inborn errors of bile acid metabolism for precision diagnosis
Authors: Nittono Hiroshi , Suzuki Mitsuyoshi , Suzuki Hiromi , Sugimoto Satoru , Mori Jun , Sakamoto Rieko , Takaki Yugo , Hayashi Hisamitsu , Takei Hajime , Kimura Akihiko
Published: April 2024

Inborn errors of bile acid metabolism (IEBAM) cause cholestasis during the neonatal period, and 8 types of IEBAM have been reported to date. IEBAM accounts for approximately 2% of cases of cholestasis of unknown cause. As only 10 patients have been identified in Japan, IEBAM presents diagnostic challenges due to the similarity of clinical symptoms with biliary atresia, thus necessitating precise differentiation to avoid unnecessary invasive procedures. Laboratory tests in IEBAM are characterized by normal γ-glutamyltransferase (GGT) and serum total bile acid (STBA) levels despite the presence of cholestasis; therefore, measuring STBA and GGT is essential to distinguishing biliary atresia from IEBAM. With suspected IEBAM, liquid chromatography–mass spectrometry (LC/MS) analysis of urinary bile acids is needed to optimize diagnostic and therapeutic efficacy and avoid open cholangiography and initiate treatment for primary bile acids such as cholic acid or chenodeoxycholic acid. This prospective report aims to increase awareness of IEBAM by highlighting the characteristics of general blood test and bile acid profiles from LC/MS analyses of blood, urine, and stool samples.
Predicting neural activity of whole body cast shadow through object cast shadow in dynamic environments
Authors: Giannopulu Irini , Lee Khai , Abdi Elahe , Noori-Hoshyar Azadeh , Brotto Gaelle , Van Velsen Mathew , Lin Tiffany , Gauchan Priya , Gorman Jazmin , Indelicato Giuseppa
Published: April 2024

Shadows, as all other objects that surround us, are incorporated into the body and extend the body mediating perceptual information. The current study investigates the hypothesis according to which the perception of object shadows would predict the perception of body shadows. 38 participants (19 males and 19 females) aged 23 years on average were immersed into a virtual reality environment and instructed to perceive and indicate the coincidence or non coincidence between the movement of a ball shadow with regard to ball movement on the one hand, and between their body shadow and their body position in space on the other. Their brain activity was recording via a 32-channel EEG system, in which beta (13.5–30 Hz) oscillations were analyzed. A series of Multiple Regression Analysis (MRA) revealed that the beta dynamic oscillations patterns of the bilateral occipito-parieto-frontal pathway associated with the perception of ball shadow appeared to be a significant predictor of the increase in beta oscillations across frontal areas related to the body shadow perception and the decrease in beta oscillations across frontal areas connected to the decision making of the body shadow. Taken together, the findings suggest that inferential thinking ability relative to body shadow would be reliably predicted from object shadows and that the bilateral beta oscillatory modulations would be indicative of the formation of predictive neural frontal assemblies, which encode and infer body shadow neural representation, that is, a substitution of the physical body.
Analysis of social metrics on scientific production in the field of emotion-aware education through artificial intelligence
Authors: Roda-Segarra Jacobo , Mengual-Andrés Santiago , Payà Rico Andrés
Published: April 2024

Research in the field of Artificial Intelligence applied to emotions in the educational context has experienced significant growth in recent years. However, despite the field’s profound implications for the educational community, the social impact of this scientific production on digital social media remains unclear. To address this question, the present research has been proposed, aiming to analyze the social impact of scientific production on the use of Artificial Intelligence for emotions in the educational context. For this purpose, a sample of 243 scientific publications indexed in Scopus and Web of Science has been selected, from which a second sample of 6,094 social impact records has been extracted from Altmetric, Crossref, and PlumX databases. A dual analysis has been conducted using specially designed software: on one hand, the scientific sample has been analyzed from a bibliometric perspective, and on the other hand, the social impact records have been studied. Comparative analysis based on the two dimensions, scientific and social, has focused on the evolution of scientific production with its corresponding social impact, sources, impact, and content analysis. The results indicate that scientific publications have had a high social impact (with an average of 25.08 social impact records per publication), with a significant increase in research interest starting from 2019, likely driven by the emotional implications of measures taken to curb the COVID-19 pandemic. Furthermore, a lack of alignment has been identified between articles with the highest scientific impact and those with the highest social impact, as well as a lack of alignment in the most commonly used terms from both scientific and social perspectives, a significant variability in the lag in months for scientific research to make an impact on social media, and the fact that the social impact of the research did not emerge from the interest of Twitter users unaffiliated with the research, but rather from the authors, publishers, or scientific institutions. The proposed comparative methodology can be applied to any field of study, making it a useful tool given that current trends in accreditation agencies propose the analysis of the repercussion of scientific research in social media.
Learning from local knowledge on changes in tree-cover and water availability: the case of the contested agroforested landscape of the Mt. Elgon Water Tower, Uganda
Authors: Kimbowa George , Buyinza Joel , Gathenya John M. , Muthuri Catherine
Published: April 2024

Participatory Rural Appraisal was used to compare stakeholder perceptions of spatial and temporal variation in tree cover and water availability using Uganda’s Mt. Elgon Water Tower as a case study. This study was guided by the research question: How do multi-level stakeholders’ perceptions of changes in tree-cover and water availability vary across spatial and temporal scales of the water tower? Five Participatory Rural Appraisal tools were applied to understand multi-level stakeholders’ perspectives on the changes in tree-cover and water availability. Data was collected from farmers’ focus group discussions, key experts from local government offices and structured household interviews with local communities. This study focused on the upper and lower zones of the water tower, which differ in terms of proximity to Mt. Elgon National Forest Park, household water-use, elevation, and tree-cover. Results showed that there are both similarities and differences in perceptions of changes in tree cover and water availability among stakeholders. Farmers and key experts perceived a decrease in water availability and a decrease in overall tree cover for the period 1990–2020. There are differences in stakeholders’ perceptions of water availability across the zones and sub-catchments. For instance, more farmers in Sipi River Sub-catchment perceived a decrease in water availability during 2006–2020 compared to 1990–2005 period. There were notable contradictions in farmers’ and key experts’ perceptions on tree cover. Farmers’ perceptions of 1990–2020 precipitation were in agreement with trends in existing precipitation data. Declining trends of natural tree cover especially outside the Mt. Elgon national forest park pose great threat to water resources originating from forests. Changes in tree cover and water availability in the study area can be partly explained by the tree species grown. Local stakeholders’ perceptions complemented the existing data gaps in explaining the changes in tree cover and water availability. Participatory Rural Appraisal tools are helpful for documenting the perceptions of local communities. However, this cannot replace reliable data resulting from large-scale efforts for monitoring changes and variations in tree-cover and water availability. The findings of this paper can be valuable to inform strategies for community involvement in sustainable agroforested landscape management.
Tool to assess the groundstroke technique of preadolescent tennis players
Authors: Diler Hakan , Şahan Asuman , Erman Kemal Alparslan
Published: April 2024

Objective

In this study, we develop a tool that can be used by tennis coaches to evaluate the groundstroke (forehand and backhand) technique of preadolescent tennis players.

Methods

The participants of the study were 60 children (30 males and 30 females) aged 10–12 years, with at least two years of training in tennis. The Groundstroke Correction Checklist (GCC) was translated into Turkish by using a blind procedure. The Turkish translation was then evaluated by 15 coaches of the Turkish Tennis Federation who had at least a level-3 coaching license and more than five years of coaching experience. The technical components related to technique in the checklist were labeled as unimportant, important, and very important. Following this, the GCC was converted into a Groundstroke Technique Assessment Test Tool (GTATT) by a selection committee consisting of three experts, and its reliability and validity were assessed by using it in the field. Spearman's correlation was used to analyze the correlation (test-r test) between the technical evaluation scores assigned to the players by the tennis coaches based on the GTATT in the first and second weeks. Intra- and inter-rater reliability was used to analyze the overall scores of technical evaluations in the first and second weeks to assess the reliability of the scale used. We determined each player's number of years of playing experience (TPY), technical evaluation (TE), international tennis-level test score (ITN), I-cord classification order (ICCO), and the number of games won (GW) in a tournament organized among themselves and evaluated the correlations among these parameters by using Spearman's correlation analysis.

Conclusion

A statistically high and significant correlation was observed between the technical evaluations of the players' forehand and backhand groundstrokes by the tennis coaches by using the GTATT in the first and second weeks (r > .90, p < .01). The analysis of the intra- and inter-rater reliability of the GTATT yielded excellent agreement between the technical observations of the three coaches of the players' forehand and backhand strokes in the first and second weeks.
Lifestyle behavior of physiotherapy students from Ecuador upon admission to higher education: a cross-sectional study
Authors: Gutiérrez-Espinoza Héctor , Cassola-Cajiao María , Garzón-Ulloa Emilia , Celi-Lalama Daniela , Bastidas-Caldes Carlos , Araya-Quintanilla Felipe , Cristi-Montero Carlos , López-Gil José Francisco
Published: April 2024

Introduction

Admission to university has been identified as a period involving the adoption of unhealthy lifestyle behaviors. However, few studies have addressed the extent of this experience among Latin American university students. The aim of this study was twofold: first, to describe anthropometric variables, body composition, physical activity, sedentary behavior, sleep duration and quality, diet, and alcohol consumption in first-year students entering physiotherapy school at the Universidad de las Americas in Quito, Ecuador; second, to test differences in these variables between sexes.

Methods

A total of 116 students were recruited. Sociodemographic variables, anthropometric indices, body composition, physical activity, sedentary behavior, sleep quality and duration, adherence to 24-hour movement guidelines, physical fitness (i.e., handgrip strength), diet, alcohol consumption, and smoking habits were evaluated.

Results

A total of 50 male (43.1%) and 66 female (56.9%) students were assessed. Overall, the adherence to the 24-hour movement guidelines of the students upon admission to university was 8.6%. Conversely, 86 students (74.1%) did not meet any of the recommendations. Upon admission to university, only 8.6% of the students (female 2.6%; male 6%) met the overall 24-hour movement guidelines. Additionally, 82 students (70.7%) needed changes in diet quality, 81 students (69.8%) had significant sleep disturbances, and 22 students (18.9%) had harmful alcohol consumption. A greater proportion of males met all three 24-h movement recommendations (p = 0.025) than females did. In addition, females reported a greater percentage of occasional smokers (p = 0.025) and a greater prevalence of obesity (p < 0.001), a lower level of physical activity (p < 0.001), and a greater percentage of sleep disturbance (p < 0.001). Conversely, males reported greater waist circumference (p = 0.005), weight (p < 0.001), handgrip strength (p < 0.001), and a greater percentage of harmful alcohol consumption (p < 0.001).

Discussion

Our study showed that upon admission to university, overall adherence to the 24-hour movement guidelines is low among university students. Additionally, a high percentage of students reported unhealthy lifestyle behaviors, with differences according to sex. Our findings could lead to the use of specific sex-specific strategies for the prevention and promotion of movement and lifestyle behaviors during this critical period.
Investigation of the food safety of lamb meat obtained in ecologically unfavorable territories
Authors: Apsalikova Zukhra , Amirkhanov Kumarbek , Gaptar Svetlana , Mukanova Alima , Yevlampiyeva Yelena , Kassymov Samat , Brait Yulia , Spanova Assem , Lipikhina Aleksandra , Apsalikov Kazbek
Published: April 2024

Introduction

This article presents data on the ecological situation of agricultural territories where small cattle are raised, focusing on the exposure dose rate, radon concentration in residential and social buildings, and the density of alpha and beta particle flux. The study also examines the levels of heavy metals (Pb, Cd, As, Hg) and radionuclides Cs-137 and Sr-90 in raw meat from ecologically unfavorable areas, as well as the effects of heat treatment on reducing the content of these harmful substances.

Methods

The research involved analyzing raw meat samples from the Abai district near the former Semipalatinsk Nuclear Test Site in Semey, Kazakhstan. The levels of heavy metals and radionuclides were measured before and after heat treatment to assess the effectiveness of cooking in reducing these contaminants. Radioecological measurements were also conducted in residential and social buildings to evaluate radon concentration and alpha and beta particle flux.

Results and discussion

The results of the study showed a significant decrease in the levels of lead, cadmium, arsenic, Cs-137, and Sr-90 in meat after heat treatment. Prolonged cooking or quenching led to the most significant reductions in toxicity and radionuclides. These findings suggest that heat treatment can be an effective method for reducing the levels of harmful substances in meat from ecologically compromised areas. Further research is needed to explore other potential strategies for mitigating the impact of environmental contamination on food safety in agricultural regions.
Developing a pragmatic consensus procedure supporting the ICH S1B(R1) weight of evidence carcinogenicity assessment
Authors: Bassan Arianna , Steigerwalt Ronald , Keller Douglas , Beilke Lisa , Bradley Paul M. , Bringezu Frank , Brock William J. , Burns-Naas Leigh Ann , Chambers Jon , Cross Kevin , Dorato Michael , Elespuru Rosalie , Fuhrer Douglas , Hall Frances , Hartke Jim , Jahnke Gloria D. , Kluxen Felix M. , McDuffie Eric , Schmidt Friedemann , Valentin Jean-Pierre , Woolley David , Zane Doris , Myatt Glenn J.
Published: April 2024

The ICH S1B carcinogenicity global testing guideline has been recently revised with a novel addendum that describes a comprehensive integrated Weight of Evidence (WoE) approach to determine the need for a 2-year rat carcinogenicity study. In the present work, experts from different organizations have joined efforts to standardize as much as possible a procedural framework for the integration of evidence associated with the different ICH S1B(R1) WoE criteria. The framework uses a pragmatic consensus procedure for carcinogenicity hazard assessment to facilitate transparent, consistent, and documented decision-making and it discusses best-practices both for the organization of studies and presentation of data in a format suitable for regulatory review. First, it is acknowledged that the six WoE factors described in the addendum form an integrated network of evidence within a holistic assessment framework that is used synergistically to analyze and explain safety signals. Second, the proposed standardized procedure builds upon different considerations related to the primary sources of evidence, mechanistic analysis, alternative methodologies and novel investigative approaches, metabolites, and reliability of the data and other acquired information. Each of the six WoE factors is described highlighting how they can contribute evidence for the overall WoE assessment. A suggested reporting format to summarize the cross-integration of evidence from the different WoE factors is also presented. This work also notes that even if a 2-year rat study is ultimately required, creating a WoE assessment is valuable in understanding the specific factors and levels of human carcinogenic risk better than have been identified previously with the 2-year rat bioassay alone.
Screening of mRNA markers in early bovine tuberculosis blood samples
Authors: Jiang Dongfeng , Song Xiaoyi , Yang Liyu , Zheng Li , Niu Kaifeng , Niu Hui
Published: April 2024

Bovine tuberculosis (bTB) is a chronic zoonotic disease caused by Mycobacterium bovis. A large number of cattle are infected with bTB every year, resulting in huge economic losses. How to control bTB is an important issue in the current global livestock economy. In this study, the original transcriptome sequences related to this study were obtained from the dataset GSE192537 by searching the Gene Expression Omnibus (GEO) database. Our differential gene analysis showed that there were obvious biological activities related to immune activation and immune regulation in the early stage of bTB. Immune-related biological processes were more active in the early stage of bTB than in the late. There were obvious immune activation and immune cell recruitment in the early stage of bTB. Regulations in immune receptors are associated with pathophysiological processes of the early stage of bTB. A gene module consisting of 236 genes significantly related to the early stage of bTB was obtained by weighted gene co-expression network analysis, and 18 hub genes were further identified as potential biomarkers or therapeutic targets. Finally, by random forest algorithm and logistic regression modeling, FCRL1 was identified as a representative mRNA marker in early bTB blood. FCRL1 has the potential to be a diagnostic biomarker in early bTB.
Construction of Maize Threshing Model by DEM Simulation
Authors: Ji Jiangtao , Jin Tianci , Li Qianwen , Wu Yuanze , Wang Xuezhen
Published: April 2024

This paper proposes a modeling method of maize in threshing. The static friction coefficient and rolling resistance coefficient of the maize grain were measured using the slope method. The maize grain stacking angle test was designed using the central composite design response surface test. A regression model was established based on the simulation results to find the best combination. The results suggested that the modeling method proposed in this paper was effective in improving the accuracy of maize grain simulation compared with previous methods. Furthermore, this paper presents a method to verify the feasibility and reliability of the maize grain cob discrete element model using the distribution of grain in the granary and the final removal rate as the verification method. The results of the actually simulated threshing test were analyzed using a Wilcoxon signed-rank test, heat map analysis, and the Spearman’s rank correlation coefficient. It was found that the DEM model of maize cob is suitable for simulating the maize threshing process. This can aid in further research on the subject.
Rearing of Native Bumblebee Species Bombus haemorrhoidalis for Greenhouse Pollination in Pakistan
Authors: Sheikh Umer Ayyaz Aslam , Ahmad Munir , Aziz Muhammad Asif , Imran Muhammad , Rahim Junaid , Roulston T’ai , Guo Shengnan , Sun Cheng
Published: April 2024

Greenhouse tomato production is a growing business worldwide, and it is strongly promoted by bumblebee pollination. Although there are over 250 bumblebee species worldwide, very few species have been reared successfully for greenhouse tomato pollination. Those successfully managed species, especially Bombus terrestris, are shipped around the world for commercial use. However, managed bumblebees are known to escape greenhouse facilities, have established local populations, spread disease to local bumblebees, and are blamed for the declines of some indigenous bee species. An alternative to shipping exotic bumblebees around the world is to develop local species for greenhouse pollination. Such an approach has the dual benefits of creating a new industry of insect rearing while reducing threats to local bee communities. In this study, we successfully reared Bombus haemorrhoidalis, which is the most common bumblebee species in Northern Pakistan, in a laboratory and compared its effectiveness as a tomato pollinator with that of commercial B. terrestris in a greenhouse. We found that the effectiveness of B. haemorrhoidalis in tomato pollination in a greenhouse is very similar to that of B. terrestris when it comes to the fruit size, number of seeds, and fruit weight. Our study provides an example of how to rear a native bumblebee species to pollinate local crops, which is a method that could potentially substitute the importation of non-ingenious bumblebees.
Positive Correlation of Lodging Resistance and Soybean Yield under the Influence of Uniconazole
Authors: Yan Chao , Shan Fuxin , Wang Chang , Lyu Xiaochen , Wu Yuanyi , Yan Shuangshuang , Ma Chunmei
Published: April 2024

Increasing planting density is one of the most effective ways to increase soybean yield, but supra-optimum density leads to an increase in the risk of lodged soybean. In this study, two varieties were selected. Heinong84 (lodging-susceptible variety, HN84) had planting densities of 200,000 plants/hm2, 300,000 plants/hm2, and 400,000 plants/hm2. Henong60 (lodging-resistant, HN60) had planting densities of 300,000 plants/hm2, 400,000 plants/hm2, and 500,000 plants/hm2. When the foliar application of uniconazole (50 mg/L) occurred at the beginning of the flowering stage (R1), the plant morphology, fiber composition, and mechanical properties of soybean internodes were determined at the podding and seed filling stages, and the yield was measured at the harvest stage. The results showed that spraying uniconazole at the R1 stage changed the morphology structure of soybean plants (i.e., plant height and petiole length reduction; stem diameter and leafstalk angle increase), improved the internode quality (i.e., increased breaking force, lignin content, cellulose content, hemicellulose content, and stem dry weight per unit length), and increased the number of grains per plant at the harvest stage. Thus, it is concluded that the application of uniconazole improved the plant population structure by changing the morphology of soybean plants, which was conducive to good light transmission and ventilation, improved the internode quality and lodging resistance, and increased the yield.
Cabbage Transplantation State Recognition Model Based on Modified YOLOv5-GFD
Authors: Sun Xiang , Miao Yisheng , Wu Xiaoyan , Wang Yuansheng , Li Qingxue , Zhu Huaji , Wu Huarui
Published: April 2024

To enhance the transplantation effectiveness of vegetables and promptly formulate subsequent work strategies, it is imperative to study the recognition approach for transplanted seedlings. In the natural and complex environment, factors like background and sunlight often hinder accurate target recognition. To overcome these challenges, this study explores a lightweight yet efficient algorithm for recognizing cabbage transplantation states in natural settings. Initially, FasterNet is integrated as the backbone network in the YOLOv5s model, aiming to expedite convergence speed and bolster feature extraction capabilities. Secondly, the introduction of the GAM attention mechanism enhances the algorithm’s focus on cabbage seedlings. EIoU loss is incorporated to improve both network convergence speed and localization precision. Lastly, the model incorporates deformable convolution DCNV3, which further optimizes model parameters and attains a superior balance between accuracy and speed. Upon testing the refined YOLOv5s target detection algorithm, improvements were evident. When compared to the original model, the mean average precision (mAP) rose by 3.5 percentage points, recall increased by 1.7 percentage points, and detection speed witnessed an impressive boost of 52 FPS. This enhanced algorithm not only reduces model complexity but also elevates network performance. The method is expected to streamline transplantation quality measurements, minimize time and labor inputs, and elevate field transplantation quality surveys’ automation levels.
Efficient Expression of Functionally Active Aflibercept with Designed N-glycans
Authors: Keshvari Tahereh , Melnik Stanislav , Sun Lin , Niazi Ali , Aram Farzaneh , Moghadam Ali , Kogelmann Benjamin , Wozniak-Knopp Gordana , Kallolimath Somanath , Ramezani Amin , Steinkellner Herta
Published: April 2024

Aflibercept is a therapeutic recombinant fusion protein comprising extracellular domains of human vascular endothelial growth factor receptors (VEGFRs) and IgG1-Fc. It is a highly glycosylated protein with five N-glycosylation sites that might impact it structurally and/or functionally. Aflibercept is produced in mammalian cells and exhibits large glycan heterogeneity, which hampers glycan-associated investigations. Here, we report the expression of aflibercept in a plant-based system with targeted N-glycosylation profiles. Nicotiana benthamiana-based glycoengineering resulted in the production of aflibercept variants carrying designed carbohydrates, namely, N-glycans with terminal GlcNAc and sialic acid residues, herein referred to as AFLIGnGn and AFLISia, respectively. Both variants were transiently expressed in unusually high amounts (2 g/kg fresh leaf material) in leaves and properly assembled to dimers. Mass spectrometric site-specific glycosylation analyses of purified aflibercept showed the presence of two to four glycoforms in a consistent manner. We also demonstrate incomplete occupancy of some glycosites. Both AFLIGnGn and AFLISia displayed similar binding potency to VEGF165, with a tendency of lower binding to variants with increased sialylation. Collectively, we show the expression of functionally active aflibercept in significant amounts with controlled glycosylation. The results provide the basis for further studies in order to generate optimized products in the best-case scenario.
A Review of Small-Scale Vapor Compression Refrigeration Technologies
Authors: Silva-Romero Juan Carlos , Belman-Flores Juan Manuel , Aceves Salvador M.
Published: April 2024

The study and development of miniature refrigeration and climate conditioning systems based on vapor compression for small-scale applications have received wide interest in recent years due to their advantages compared with other available technologies, both active and passive. This paper identifies different applications and areas of opportunity, including electronic components and personal cooling, where small-scale vapor compression refrigeration systems are anticipated to play a key role in technological development. This paper presents the current state of the art, including applications, component designs, operating conditions, experiments, published results, etc. to describe the current status of small-scale vapor compression refrigeration and illustrate a perspective for the future of this technology.
Dynamic Response of the Tunnel Lining with a Circumferential Crack Subjected to a Harmonic Point Load
Authors: Yang Jianwei , Liu Changdong , Liu Peishan , Zhao Yue
Published: April 2024

Cracks are one of the most common diseases of tunnel lining, and the structural dynamic response can be used to assess the health of a tunnel. Hence, this paper investigates the dynamic response of shield tunnel lining with a partly circumferential crack. The shield tunnel lining is regarded as a thin cylindrical shell and analyzed independently. The research methodology integrates the wave propagation method, the local flexibility matrix, the line spring model and the wave superposition principle. The results show that the position and depth of a partly circumferential crack can influence the natural frequency of the shield tunnel lining. Under the fixed-position load, as the distance from the monitoring point to the crack increases, the difference in displacement response amplitude between the undamaged and cracked linings diminishes. Moreover, deepening cracks enlarge the magnitude of amplitude differences. When the load approaches the crack, the radial amplitude difference first increases and then decreases as the monitor moves away from the crack. This finding helps determine the required monitor position. The displacement response of the selected monitor indicates that the closer the load position is to the crack, the larger the amplitude difference. The results aid in identifying the crack position and selecting corresponding load and monitor locations.
Characteristics of Changes in Contractile Properties of Muscle after Anaerobic Exercise
Authors: Dydek Justyna , Bliźniak Katarzyna , Sarmento Hugo , Ihle Andreas , Gouveia Élvio Rúbio , Iskra Janusz , Przednowek Krzysztof
Published: April 2024

Tensiomyography (TMG) has been described as an effective method for identifying differences in muscle response to athletic training stimuli. However, there is a lack of studies that evaluated changes in muscle contractile properties in response to anaerobic effort and related these changes to Wingate test parameters in more than one lower limb superficial muscle. The main contribution of this work is to evaluate changes in the contractile properties of muscles in response to anaerobic exercise. In a group of 20 physically active men, the body composition analysis was performed using a Tanita DC-360 device. The anaerobic effort was assessed by a Wingate test (30 s), and the contractile properties of muscles were measured using a TMG S2 device. The TMG parameters were measured in selected superficial muscles of the dominant lower limb. The study also calculated indices of the differences (d) and the size of the effect (r). An anaerobic effort in the form of the Wingate test resulted in greater stiffness (Dm) in the vastus lateralis muscle (p = 0.0365; r = 0.47) and a delayed response to stimulus (Td) in the vastus lateralis (p = 0.0239; r = 0.51) and vastus medialis (p = 0.0031; r = 0.66) muscles. The half relaxation time (Tr) (p = 0.0478; r = 0.44) and sustained contraction time (Ts) (p = 0.0276; r = 0.49) in the rectus femoris muscle were also increased. In contrast, a prolonged stimulus-response time (Td) with a decrease in sustained contraction time (Ts) was observed in the gastrocnemius lateralis (Td: p = 0.0054; r = 0.64 and Ts: p = 0.0012; r = 0.74) and gastrocnemius medialis (Td: p = 0.0229; r = 0.52 and Ts: p = 0.0054; r = 0.64) muscles. A significant decrease in contraction time (Tc) (p = 0.0051; r = 0.63) occurred only in the soleus muscle. In addition, significant correlations were shown between selected changes in contractile properties of muscle and parameters of the Wingate anaerobic test. Anaerobic exercise has a significant effect on changes in skeletal muscle contractility parameters. TMG is an effective method for identifying differences in muscle response to sports training stimuli.
Deformation Patterns and Failure Mechanisms of Soft-Hard-Interbedded Anti-Inclined Layered Rock Slope in Wolong Open-Pit Coal Mine
Authors: Chen Guohong , Cai Peng , Zhan Jiewei , Yang Yueqiao , Yao Zhaowei , Yu Zhaoyue
Published: April 2024

Since the beginning of spring 2022, successive landslides have occurred in the eastern pit slope of the Wolong Coal Mine in Qipanjing Town, Otog Banner, Inner Mongolia, which has adversely affected the mine’s production safety. This study aims to reveal the deformation patterns and failure mechanisms of landslides. Firstly, this study establishes the stratigraphic structure of the eastern pit slope of the Wolong Coal Mine using extensive field geological surveys combined with unmanned aerial vehicle photography, drilling, and comprehensive physical exploration techniques. Indoor geotechnical tests and microscopic experiments reveal that rock mass typically exhibits the characteristics of expansibility and water sensitivity. Moreover, the mechanical parameters of the rock mass were determined using a combination of the window sampling method, the Geological Strength Index, and the Hoek–Brown strength criterion estimation theory. Finally, this study consolidates the previously mentioned insights and employs FLAC3D (7.0) software to assess the stress–strain characteristics of the excavated slope. The results indicate that the deformation mode of the Wolong open pit coal mine is the toppling failure of soft-hard-interbedded anti-inclined layered rock slopes. The unloading effect and rock expansion-induced softening lead to stress concentration at the slope corners and more substantial deformation, thereby accelerating upper slope deformation. The deformation and destabilization process of landslides is categorized into four stages: the initial deformation stage, the development stage of lateral shear misalignment, the development stage of horizontal tensile-shear damage, and the slip surface development to the preslip stage. This research offers valuable references and engineering insights for future scientific investigations and the prevention of similar slope-related geological hazards.