bluuebunny/crossref_metadata_2025 huggingface dataset:
Epidemiology of interstitial lung diseases and their progressive-fibrosing behaviour in six European countries
Authors: Hilberg Ole , Hoffmann-Vold Anna-Maria , Smith Vanessa , Bouros Demosthenes , Kilpeläinen Maritta , Guiot Julien , Morais Antonio , Clemente Susana , Daniil Zoe , Papakosta Despina , Fretheim Havard , Neves Sofia , Alfaro Tiago M. , Antoniou Katerina M. , Valveny Neus , Asijee Guus , Soulard Stéphane , Wuyts Wim ,   the PERSEIDS study group, Brusselle Guy , Tzilas Vassilios , Tzouvelekis Argyris , Bouros Evangelos , Perkonoja Katariina , Henket Monique , Cunin Marie-Pierre , Rosa Raquel , Martín Teresa , Branco Joana , Alvarenga Maria , Dimeas Ilias , Simopoulou Theodora , Fouka Evangelia , Tsiouprou Ioanna , Zaharias Athanasios , Campainha Sergio , Nogueira Carla , Freitas Sara , Fernandes Vânia , Cabo Carolina , Vasarmidi Irini
Published: December 2021

The PERSEIDS study aimed to estimate incidence/prevalence of interstitial lung diseases (ILDs), fibrosing interstitial lung diseases (F-ILDs), idiopathic pulmonary fibrosis (IPF), systemic sclerosis-associated ILD (SSc-ILD), other non-IPF F-ILDs and their progressive-fibrosing (PF) forms in six European countries, as current data are scarce.

This retrospective, two-phase study used aggregate data (2014–2018). In Phase 1, incident/prevalent cases of ILDs above were identified from clinical databases through an algorithm based on codes/keywords, and incidence/prevalence was estimated. For non-IPF F-ILDs, the relative percentage of subtypes was also determined. In Phase 2, a subset of non-IPF F-ILD cases was manually reviewed to determine the percentage of PF behaviour and usual interstitial pneumonia-like (UIP-like) pattern. A weighted mean percentage of progression was calculated for each country and used to extrapolate incidence/prevalence of progressive-fibrosing ILDs (PF-ILDs).

In 2018, incidence/105person-years ranged between 9.4 and 83.6 (ILDs), 7.7 and 76.2 (F-ILDs), 0.4 and 10.3 (IPF), 6.6 and 71.7 (non-IPF F-ILDs), and 0.3 and 1.5 (SSc-ILD); and prevalence/105persons ranged between 33.6 and 247.4 (ILDs), 26.7 and 236.8 (F-ILDs), 2.8 and 31.0 (IPF), 22.3 and 205.8 (non-IPF F-ILDs), and 1.4 and 10.1 (SSc-ILD). Among non-IPF F-ILDs, sarcoidosis was the most frequent subtype. PF behaviour and UIP-like pattern were present in a third of non-IPF F-ILD cases each and hypersensitivity pneumonitis showed the highest percentage of progressive behaviour. Incidence of PF-ILDs ranged between 2.1 and 14.5/105person-years, and prevalence between 6.9 and 78.0/105persons.

To our knowledge, PERSEIDS is the first study assessing incidence, prevalence and rate of progression of ILDs across several European countries. Still below the threshold for orphan diseases, the estimates obtained were higher and more variable than reported in previous studies, but differences in study design/population must be considered.
Variation characteristics of ocean sediment Fe levels and their relationship with grain sizes in culture areas over a long period
Authors: Zhang Baoyi , Li Manyi , An Maoguo , Zhi Chenglong , Li Qingcai , Zhang Yingmei , Han Shuangyuan , Zheng Haitao , Li Jun , Chen Huaixin , Chen Qiao
Published: December 2021

Iron (Fe) is an essential component for marine ecosystems, and it is related to the growth of phytoplankton communities and environmental evolution in coastal area. However, the effect of aquaculture activities on sediment Fe levels is not well studied. Fe levels and grain sizes are determined in two cores (respectively Core C in the culture area and Core A in the control area) in Sishili Bay to reveal the influence of cultivation on sediment Fe levels over an extended period. The sediment Fe levels are distinguished in the upper sections (culture period) but equal in the lower sections (non-culture period) of the two cores. The core C has the same Fe levels as Core A before 1950s (non-culture period). However, the sediment Fe levels of Core C increased during 1950s–1970s (the algae culture period) and decreased after the 1970s (shellfish culture period) compared with Core A, indicating the algae and shellfish culture impose opposite effects on sediment Fe levels. Similarly, sediment grain sizes are observed to be finer during the algae culture period but coarser during the shellfish culture period, and the variation of sediment grain sizes because of culture activities is the important factor affecting sediment Fe levels. The slowing down of ocean current due to algae culture causes finer particles and higher Fe levels in sediment. However, during the shellfish culture period, bio-deposition and re-suspension play major roles in coarsening sediment particles and decreasing sediment Fe levels.
Is there a relationship between serum vitamin D and semen parameters? A cross-sectional sample of the Iranian infertile men
Authors: Hajianfar Hossein , Karimi Elham , Mollaghasemi Negar , Rezaei Sheyda , Arab Arman
Published: December 2021

Background

Recent studies suggest that serum vitamin D may be associated with semen parameters. In the present cross-sectional study, we attempted to investigate the association between serum vitamin D levels and semen parameters among Iranian sub-fertile men.

Results

A total of 350 infertile men recruited for this cross-sectional study using a simple random sampling method with a mean age of 34.77 years old, body mass index of 26.67 kg/m2, serum vitamin D of 20.17 ng/ml, semen volume of 3.82 mL, sperm count of 44.48 (106/mL), sperm total motility of 38.10 %, and morphologically normal sperm of 7.0 %. After controlling for potential confounders, serum vitamin D was positively associated with semen volume (β = 0.63, 95 % CI: 0.06, 1.20), sperm count (β = 14.40, 95 % CI: 4.56, 24.25), sperm total motility (β = 18.12, 95 % CI: 12.37, 23.86), and sperm normal morphology (β = 1.95, 95 % CI: 1.07, 2.83).

Conclusions

The present findings suggest that higher serum vitamin D levels are positively associated with higher semen volume, sperm count, sperm total motility, and normal morphology rate. These findings, however, do not specify a cause-and-effect relationship, and there is a need for further research in this area to understand whether vitamin D supplementation can improve semen parameters.
A Metabolomics approach for the diagnosis Of SecondAry InfeCtions in COVID-19 (MOSAIC): a study protocol
Authors: McCreath Gordan , Whitfield Phillip D. , Roe Andrew J. , Watson Malcolm J. , Sim Malcolm A. B.
Published: December 2021

Background

Critically ill patients with COVID-19 are at an increased risk of developing secondary bacterial infections. These are both difficult to diagnose and are associated with an increased mortality. Metabolomics may aid clinicians in diagnosing secondary bacterial infections in COVID-19 through identification and quantification of disease specific biomarkers, with the aim of identifying underlying causative microorganisms and directing antimicrobial therapy.

Methods

This is a multi-centre prospective diagnostic observational study. Patients with COVID-19 will be recruited from critical care units in three Scottish hospitals. Three serial blood samples will be taken from patients, and an additional sample taken if a patient shows clinical or microbiological evidence of secondary infection. Samples will be analysed using LC–MS and subjected to bioinformatic processing and statistical analysis to explore the metabolite changes associated with bacterial infections in COVID-19 patients. Comparisons of the data sets will be made with standard microbiological and biochemical methods of diagnosing infection.

Discussion

Metabolomics analyses may provide additional strategies for identifying secondary infections, which might permit faster initiation of specific tailored antimicrobial therapy to critically ill patients with COVID-19.
Avelumab as neoadjuvant therapy in patients with urothelial non-metastatic muscle invasive bladder cancer: a multicenter, randomized, non-comparative, phase II study (Oncodistinct 004 - AURA trial)
Authors: Martinez Chanza Nieves , Soukane Louisa , Barthelemy Philippe , Carnot Aurélien , Gil Thierry , Casert Vinciane , Vanhaudenarde Vincent , Sautois Brieuc , Staudacher Lionel , Van den Brande Jan , Culine Stephane , Seront Emmanuel , Gizzi Marco , Albisinni Simone , Tricard Thibault , Fantoni Jean Christophe , Paesmans Marianne , Caparica Rafael , Roumeguere Thierry , Awada Ahmad
Published: December 2021

Introduction

Cisplatin-based neoadjuvant chemotherapy (NAC) followed by surgery is the standard treatment for patients with non-metastatic muscle invasive bladder cancer (MIBC). Unfortunately, many patients are not candidates to receive cisplatin due to renal impairment. Additionally, no predictive biomarkers for pathological complete response (pCR) are currently validated in clinical practice. Studies evaluating immune checkpoint inhibitors in the peri-operative setting are emerging with promising results. Clinical trials are clearly required in the neoadjuvant setting in order to improve therapeutic strategies.

Methods and analysis

Oncodistinct 004 – AURA is an ongoing multicenter phase II randomized trial assessing the efficacy and safety of avelumab single-agent or combined to different NAC regimens in patients with non-metastatic MIBC. Patients are enrolled in two distinct cohorts according to their eligibility to receive cisplatin-based NAC. In the cisplatin eligible cohort, patients are randomized in a 1:1 fashion to receive avelumab combined with cisplatin-gemcitabine or with dose-dense methotrexate-vinblastine-doxorubicin-cisplatin. In the cisplatin ineligible cohort, patients are randomized at a 1:1 ratio to paclitaxel-gemcitabine associated to avelumab or avelumab alone. Primary endpoint is pCR. Secondary endpoints are pathological response and safety.

Ethics and dissemination

The study is approved by ethics committee from all participating centers. All participants provide informed consent prior inclusion to the study. Once completed, results will be published in peer-reviewed journals.

Trial registration number

ClinicalTrials.gov (NCT03674424).
Exosomal microRNA-107 reverses chemotherapeutic drug resistance of gastric cancer cells through HMGA2/mTOR/P-gp pathway
Authors: Jiang Lu , Zhang Yan , Guo Linghui , Liu Chaoyang , Wang Pan , Ren Weihong
Published: December 2021

Background

RNA cargo in exosomes, especially microRNAs (miRNAs), play an important role in the chemotherapy drug resistance of human cancers. However, the role and mechanism of exosomal miR-107 on multidrug resistance of gastric cancer cells was still not clear. In this study, we sought to explore whether exosomal miR-107 could reverse the resistance of gastric cancer cells to the chemotherapy drugs.

Methods

We extracted exosomes from sensitive (SGC-7901, MGC-803) and resistant (SGC-7901/5-FU) gastric cancer cells by ultracentrifugation and the isolated exosomes were identified using transmission electron microscopy (TEM) and dynamic light scattering analysis (DLS). The expression of miR-107 and high mobility group A2 (HMGA2) were detected by real-time quantitative PCR (RT-qPCR). MTT assay was used to investigate the effect of exosomes on gastric cancer cells growth in vitro. The uptake of exosomes by recipient cells were observed using a fluorescence microscope. The predicted target relationship between miR-107 and HMGA2 was verified by gauss-luciferase reporter assay. The expression of HMGA2, p-mTOR/mTOR, P-gp and other exosomal indicated marker proteins was detected by western blot.

Results

Our results indicated that the isolated exosomes were typically cup-like lipid bilayer membranes structure. SGC-7901/5-FU cells were cross-resistant to chemotherapy drug cisplatin (CDDP), and the sensitive cells-secreted exosomes drastically reversed the resistance of the resistant GC cells to the chemotherapeutic drugs, which was verified by exosomal inhibitor GW4896. Mechanistically, the reversal effect was mainly mediated by exosome-secreted miR-107 through downregulating the expression of target molecular HMGA2 and inhibiting HMGA2/mTOR/P-gp pathway, which were supported by results from luciferase reporter assay and rescue assay.

Conclusions

These findings demonstrated that exosome-transmitted miR-107 significantly enhanced the sensitivity of resistant gastric cancer cells to chemotherapeutic agents by mediating the HMGA2/mTOR/P-gp axis and exosomal miR-107 may be a novel target in gastric cancers treatment.
Nutritional status and associated factors among children with congenital heart disease in selected governmental hospitals and cardiac center,Addis Ababa Ethiopia
Authors: Woldesenbet Rediet , Murugan Rajalakshmi , Mulugeta Feven , Moges Tamirat
Published: December 2021

Background

Children with congenital heart disease are at risk for poor growth and under-nutrition compared with healthy children. The aim of this study was to assess the nutritional status of children with congenital heart disease and associated factors in selected governmental hospitals and cardiac center Addis Ababa, Ethiopia.

Method

Institutional based cross sectional study among 373 children aged under15 years was conducted from February to March; 2021G.c. Data was collected using structured questionnaire and chart review. Z-scores based on WHO reference ranges were used. Anthropometric z-scores based on WHO 2007 reference ranges were generated for each child. Weight-for-age z-scores for children 0–10 years and height-for-age and BMI-for-age z-scores for all children. Binary logistic regression was used for associated factors.

Result

A total of 373 children were participated in this study. The prevalence of wasting and stunting was 144(38.6%) and 134(35.9%) respectively. The prevalence of underweight and malnutrition in children under 10 years was 143(43.1%). Most of the children were diagnosed with VSD (36.7%). Children age group of 13 months-5 years were associated with wasting and underweight [AOR = 0.434, 95%CI: (0.231, 0.816)] and [AOR = 0.360, 95%CI: (0.183, 0.711)] respectively. Children diagnosed with PAH were 1.885 times more likely to be underweight [AOR = 1.885, 95%CI: (1.094, 3.246)]. When the hemoglobin level increases by every unit per g/dl the chance to be wasting and underweight decreases by 13.1 and 18.6%[AOR = 0.869, 95%CI: (0.792, 0.955)] and [AOR = 0.869, 95%CI: (0.792, 0.955)] respectively. The level of SPO2 is associated with stunting and underweight [AOR = 0.970, 95%CI: (0.943, 0.998)] and [AOR = 0.970, 95%CI: (0.943, 0.998)] respectively.

Conclusion

The prevalence of malnutrition in children with CHD is pretty high. Decreased level of hemoglobin and SPO2 was found to be associated factors for malnutrition in this case. There need to be a new strategy about including different health professional while care giving.
Novel screening model of obstructive sleep apnea for snorers with suspected NAFLD undergoing liver sonography
Authors: Lu Yang-Bor , Weng Yu-Chieh , Huang Yung-Ning , Huang Hsiung-Ying , Cheng Pei-Ting , Hsieh Hui-Shan , Tsai Ming-Shao
Published: December 2021

Background/aims

Given the increased incidence of obstructive sleep apnea (OSA) among patients with nonalcoholic fatty liver disease (NAFLD), noninvasive screening methods are urgently needed to screen for OSA risk in these patients when conducting an office-based assessment of hepatic steatosis. Therefore, we investigated the controlled attenuation parameter (CAP) and hepatic steatosis index (HSI) in patients with and without OSA and developed screening models to detect OSA.

Methods

We retrospectively reviewed the medical records of all adult snorers with suspected NAFLD undergoing liver sonography between June 2017 and June 2020. Records encompassed CAP and HSI data as well as data collected during in-hospital full-night polysomnography. The multivariate logistic regression models were constructed to explore the predictors of OSA risk. Furthermore, model validation was performed based on the medical records corresponding to the July 2020–June 2021 period.

Results

A total of 59 patients were included: 81.4% (48/59) were men, and the mean body mass index (BMI) was 26.4 kg/m2. Among the patients, 62.7% (37/59) and 74.6% (44/59) (detected by the HSI and CAP, respectively) had NAFLD, and 78% (46/59) were diagnosed with OSA on the basis of polysomnography. Three screening models based on multivariate analysis were established. The model combining male sex, a BMI of > 24.8, and an HSI of > 38.3 screened for OSA risk the most accurately, with an area under the receiver operating characteristic curve of 0.81 (sensitivity: 78%; specificity: 85%; and positive and negative predictive values: 95% and 52%, respectively) in the modeling cohort. An accuracy of 70.0% was achieved in the validation group.

Conclusions

The combination screening models proposed herein provide a convenient, noninvasive, and rapid screening tool for OSA risk and can be employed while patients receive routine hepatic check-ups. These models can assist physicians in identifying at-risk OSA patients and thus facilitate earlier detection and timely treatment initiation.
Risk factor analysis of intravesical recurrence after retroperitoneoscopic nephroureterectomy for upper tract urothelial carcinoma
Authors: Yanagi Masato , Hamasaki Tsutomu , Akatsuka Jun , Endo Yuki , Takeda Hayato , Kondo Yukihiro
Published: December 2021

Background

One of the major concerns of patients with upper tract urothelial carcinoma (UTUC) treated with nephroureterectomy is intravesical recurrence (IVR). The purpose of the present study was to investigate the predictive risk factors for IVR after retroperitoneoscopic nephroureterectomy (RNU) for UTUC.

Methods

Clinicopathological and surgical information were collected from the medical records of 73 patients treated with RNU for non-metastatic UTUC, without a history of or concomitant bladder cancer. The association between IVR after RNU and clinicopathological and surgery-related factors, including preoperative urine cytology and pneumoretroperitoneum time, was analyzed using the Fisher exact test.

Results

During the median follow-up time of 39.1 months, 18 (24.7%) patients had subsequent IVR after RNU. The 1- and 3-year IVR-free survival rates were 85.9% and 76.5%, respectively. The Fisher exact test revealed that prolonged pneumoretroperitoneum time of ≥ 210 min was a risk factor for IVR in 1 year after RNU (p = 0.0358) and positive urine cytology was a risk factor for IVR in 3 years after RNU (p = 0.0352).

Conclusions

In UTUC, the occurrences of IVR in 1 and 3 years after RNU are highly probable when the pneumoretroperitoneum time is prolonged (≥ 210 min) and in patients with positive urine cytology, respectively. Strict follow-up after RNU is more probable recommended for these patients.
Teaching the technical performance of bronchoscopy to residents in a step-wise simulated approach: factors supporting learning and impacts on clinical work – a qualitative analysis
Authors: Eickelmann Anne Kathrin , Waldner Noemi Jelena , Huwendiek Sören
Published: December 2021

Background

The ability to perform a bronchoscopy is a valuable clinical skill for many medical specialities. Learning this skill is demanding for residents, due to the high cognitive load. Lessons learned from cognitive load theory might provide a way to facilitate this learning. The aim of this study was to investigate residents’ perception of factors that support and hinder learning, as well as outcome and acceptance of a workshop on flexible bronchoscopy.

Methods

Three half-day workshops were designed to teach 12 residents the basics of handling a flexible bronchoscope. They consisted of four phases that alternated between short theoretical aspects and longer practical situations. The practical phases focussed initially on manoeuvring a bronchoscope through holes in panels inside a box, and then on examination and practice using a three-dimensional printed model of the bronchial tree. Afterwards, three audio- and video-recorded focus groups were conducted, transcribed and coded, and underwent reflexive thematic analysis.

Results

Analysis of the focus groups defined two themes: (1) factors that supported a safe and positive learning environment were optimised for intrinsic load, simulated setting, absence of pressure, dyad practice (working in pairs), small group sizes and playful learning; and (2) impacts on clinical work were perceived as high levels of learning and improved patient safety. The residents did not report factors that hindered their learning. Some suggestions were made to improve the set-up of the wooden box.

Conclusions

The half-day workshop was designed according to several factors, including cognitive load theory in a simulated setting, and creation of a safe and positive learning environment. The residents perceived this as supporting learning and patient safety. Further studies can be designed to confirm these results in a quantitative setting.

Trial registration

This study was not interventional, therefore was not registered.
A nomogram model for predicting prognosis of obstructive colorectal cancer
Authors: Lv Jian , Liu Yuan yuan , Jia Yi tao , He Jing li , Dai Guang yao , Guo Peng , Zhao Zhao long , Zhang Yan ni , Li Zhong xin
Published: December 2021

Background

The prognosis of obstructive colorectal cancer (oCRC) is worse than that of nonobstructive colorectal cancer. However, no previous study has established an individualized prediction model for the prognosis of patients with oCRC. We aimed to screen the factors that affect the prognosis of oCRC and to use these findings to establish a nomogram model that predicts the individual prognosis of patients with oCRC.

Methods

This retrospective study collected data of 181 patients with oCRC from three medical hospitals between February 2012 and December 2017. Among them, 129 patients from one hospital were used as the training cohort. Univariate and multivariate analyses were used in this training cohort to select independent risk factors that affect the prognosis of oCRC, and a nomogram model was established. The other 52 patients from two additional hospitals were used as the validation cohort to verify the model.

Results

Multivariate analysis showed that carcinoembryonic antigen level (p = 0.037, hazard ratio [HR] = 2.872 [1.065–7.740]), N stage (N1 vs. N0, p = 0.028, HR = 3.187 [1.137–8.938]; N2 vs. N0, p = 0.010, HR = 4.098 [1.393–12.051]), and surgical procedures (p = 0.002, HR = 0.299 [0.139–0.643]) were independent prognostic factors of overall survival in patients with oCRC. These factors were used to construct the nomogram model, which showed good concordance and accuracy.

Conclusion

Carcinoembryonic antigen, N stage, and surgical method are independent prognostic factors for overall survival in patients with oCRC, and the nomogram model can visually display these results.
Therapeutic implications of cancer gene amplifications without mRNA overexpression: silence may not be golden
Authors: Boichard Amélie , Lippman Scott M. , Kurzrock Razelle
Published: December 2021

Amplifications of oncogenic genes are often considered actionable. However, not all patients respond. Questions have therefore arisen regarding the degree to which amplifications, especially non-focal ones, mediate overexpression. We found that a subset of high-level gene amplifications (≥ 6 copies) (from The Cancer Genome Atlas database) was not over-expressed at the RNA level. Unexpectedly, focal amplifications were more frequently silenced than non-focal amplifications. Most non-focal amplifications were not silenced; therefore, non-focal amplifications, if over-expressed, may be therapeutically tractable. Furthermore, specific silencing of high-level focal or non-focal gene amplifications may explain resistance to drugs that target the relevant gene product.
Upregulation of ERK-EGR1-heparanase axis by HDAC inhibitors provides targets for rational therapeutic intervention in synovial sarcoma
Authors: Lanzi Cinzia , Favini Enrica , Dal Bo Laura , Tortoreto Monica , Arrighetti Noemi , Zaffaroni Nadia , Cassinelli Giuliana
Published: December 2021

Background

Synovial sarcoma (SS) is an aggressive soft tissue tumor with limited therapeutic options in advanced stage. SS18-SSX fusion oncogenes, which are the hallmarks of SS, cause epigenetic rewiring involving histone deacetylases (HDACs). Promising preclinical studies supporting HDAC targeting for SS treatment were not reflected in clinical trials with HDAC inhibitor (HDACi) monotherapies. We investigated pathways implicated in SS cell response to HDACi to identify vulnerabilities exploitable in combination treatments and improve the therapeutic efficacy of HDACi-based regimens.

Methods

Antiproliferative and proapoptotic effects of the HDACi SAHA and FK228 were examined in SS cell lines in parallel with biochemical and molecular analyses to bring out cytoprotective pathways. Treatments combining HDACi with drugs targeting HDACi-activated prosurvival pathways were tested in functional assays in vitro and in a SS orthotopic xenograft model. Molecular mechanisms underlying synergisms were investigated in SS cells through pharmacological and gene silencing approaches and validated by qRT-PCR and Western blotting.

Results

SS cell response to HDACi was consistently characterized by activation of a cytoprotective and auto-sustaining axis involving ERKs, EGR1, and the β-endoglycosidase heparanase, a well recognized pleiotropic player in tumorigenesis and disease progression. HDAC inhibition was shown to upregulate heparanase by inducing expression of the positive regulator EGR1 and by hampering negative regulation by p53 through its acetylation. Interception of HDACi-induced ERK-EGR1-heparanase pathway by cell co-treatment with a MEK inhibitor (trametinib) or a heparanase inhibitor (SST0001/roneparstat) enhanced antiproliferative and pro-apoptotic effects. HDAC and heparanase inhibitors had opposite effects on histone acetylation and nuclear heparanase levels. The combination of SAHA with SST0001 prevented the upregulation of ERK-EGR1-heparanase induced by the HDACi and promoted caspase-dependent cell death. In vivo, the combined treatment with SAHA and SST0001 potentiated the antitumor efficacy against the CME-1 orthotopic SS model as compared to single agent administration.

Conclusions

The present study provides preclinical rationale and mechanistic insights into drug combinatory strategies based on the use of ERK pathway and heparanase inhibitors to improve the efficacy of HDACi-based antitumor therapies in SS. The involvement of classes of agents already clinically available, or under clinical evaluation, indicates the transferability potential of the proposed approaches.
Point-of-care ultrasound in cardiorespiratory arrest (POCUS-CA): narrative review article
Authors: Ávila-Reyes Diana , Acevedo-Cardona Andrés O. , Gómez-González José F. , Echeverry-Piedrahita David R. , Aguirre-Flórez Mateo , Giraldo-Diaconeasa Adrian
Published: December 2021

The POCUS-CA (Point-of-care ultrasound in cardiac arrest) is a diagnostic tool in the Intensive Care Unit and Emergency Department setting. The literature indicates that in the patient in a cardiorespiratory arrest it can provide information of the etiology of the arrest in patients with non-defibrillable rhythms, assess the quality of compressions during cardiopulmonary resuscitation (CPR), and define prognosis of survival according to specific findings and, thus, assist the clinician in decision-making during resuscitation. This narrative review of the literature aims to expose the usefulness of ultrasound in the setting of cardiorespiratory arrest as a tool that allows making a rapid diagnosis and making decisions about reversible causes of this entity. More studies are needed to support the evidence to make ultrasound part of the resuscitation algorithms. Teamwork during cardiopulmonary resuscitation and the inclusion of ultrasound in a multidisciplinary approach is important to achieve a favorable clinical outcome.
Repetitive and restricted behaviours and anxiety in autism spectrum disorder: protocol for a systematic review and meta-analysis
Authors: Sellick Tegan , Ure Alexandra , Williams Katrina
Published: December 2021

Background

Autism spectrum disorder (ASD) is a neurodevelopmental disorder defined by persistent deficits in social functioning and the presence of restricted and repetitive behaviours (RRBs). RRBs refer to four subtypes of behaviour including repetitive movements, speech, or use of objects; insistence on sameness; restricted interests; and sensory processing abnormalities. Many individuals with ASD also experience anxiety, which compounds ASD-related difficulties and inhibits daily functioning. RRBs have been found to be positively associated with anxiety; however, our understanding of the interplay between RRB subtypes and anxiety remains unclear. Thus, the current review aims to clarify the association between RRBs and anxiety by conducting a systematic review and meta-analysis.

Methods

To identify relevant studies, we will search five databases: CINAHL Plus, Cochrane Central Register of Controlled Trials, Ovid MEDLINE, PsycINFO, and Scopus. Articles included in the review will have their titles, abstracts, and full texts reviewed by two independent authors and their methodological quality assessed via the modified Newcastle-Ottawa Scale. Random-effects meta-analyses will then be conducted to calculate the pooled association between RRB subtypes and anxiety. Sensitivity analyses will also be conducted to assess the potential impact of bias, missing data, outliers, and methodological differences on this relationship. Additionally, this review will collate the factors which may influence the anxiety-RRB relationship to help identify who is most vulnerable to developing anxiety.

Discussion

This will be the first review to examine the association between the four subtypes of RRBs and anxiety in individuals with ASD. Understanding this relationship, and the factors associated with this, may help clinicians understand the different underpinnings and presentations of anxiety within this population with potential implications for assessment and treatment.

Systematic review registration

PROSPERO CRD42020185434
Appropriate number of observations for determining hand hygiene compliance among healthcare workers
Authors: Park Se Yoon , Park Suyeon , Hwang Beom Seuk , Lee Eunjung , Kim Tae Hyong , Won Sungho
Published: December 2021

We sought to determine the minimum number of observations needed to determine hand hygiene (HH) compliance among healthcare workers. The study was conducted at a referral hospital in South Korea. We retrospectively analyzed the result of HH monitoring from January to December 2018. HH compliance was calculated by dividing the number of observed HH actions by the total number of opportunities. Optimal HH compliance rates were calculated based on adherence to the six-step technique recommended by the World Health Organization. The minimum number of required observations (n) was calculated by the following equation using overall mean value (ρ), absolute precision (d), and confidence interval (CI) (1 − α) [the equation: $${\text{n}} \ge Z_{\alpha /2}^{2} \times \rho \times \left( {1 - \rho } \right)/d^{2}$$

n
≥

Z

α
/
2

2

×
ρ
×

1
-
ρ

/

d
2

]. We considered ds of 5%, 10%, 20%, and 30%, with CIs of 99%, 95%, and 90%. During the study period, 8791 HH opportunities among 1168 healthcare workers were monitored. Mean HH compliance and optimal HH compliance rates were 80.3% and 59.7%, respectively. The minimum number of observations required to determine HH compliance rates ranged from 2 ($$d$$
d
: 30%, CI: 90%) to 624 ($$d$$
d
: 5%, CI: 99%), and that for optimal HH compliance ranged from 5 ($$d$$
d
: 30%, CI: 90%) to 642 ($$d$$
d
: 5%, CI: 99%). Therefore, we found that our hospital required at least five observations to determine optimal HH compliance.
Poultry gut health – microbiome functions, environmental impacts, microbiome engineering and advancements in characterization technologies
Authors: Aruwa Christiana Eleojo , Pillay Charlene , Nyaga Martin M. , Sabiu Saheed
Published: December 2021

The gastrointestinal tract (GIT) health impacts animal productivity. The poultry microbiome has functions which range from protection against pathogens and nutrients production, to host immune system maturation. Fluctuations in the microbiome have also been linked to prevailing environmental conditions. Healthy poultry birds possess a natural resistance to infection. However, the exploration of environmental impacts and other relevant factors on poultry growth and health have been underplayed. Since good performance and growth rate are central to animal production, the host-microbiome relationship remains integral. Prior to the emergence of metagenomic techniques, conventional methods for poultry microbiome studies were used and were low-throughput and associated with insufficient genomic data and high cost of sequencing. Fortunately, the advent of high-throughput sequencing platforms have circumvented some of these shortfalls and paved the way for increased studies on the poultry gut microbiome diversity and functions. Here, we give an up-to-date review on the impact of varied environments on microbiome profile, as well as microbiome engineering and microbiome technology advancements. It is hoped that this paper will provide invaluable information that could guide and inspire further studies on the lingering pertinent questions about the poultry microbiome.
Pharmacists’ perceptions and attitudes toward drug importation into the State of Florida
Authors: Hertig John B. , Jochem Jade M. , Long Allissa M.
Published: December 2021

Background

The Department of Health and Human Services and the Food and Drug Administration released the Safe Importation Action Plan in July 2020 detailing methods to import medicines from Canada to combat increasing drug costs. In November 2020, Florida became the first state in the United States to create and propose an importation plan from Canada. This study examines the proposal submitted by Florida, Florida pharmacists’ perceptions of the program on patient safety, and Florida pharmacists’ thoughts on the pharmacy operational impact.

Methods

This was a cross-sectional study utilizing an electronic questionnaire sent to pharmacist members of the Florida Pharmacy Association. The survey incorporated closed-ended and open-ended questions. The results from the study were reported and analyzed through descriptive statistics, qualitative and quantitative data.

Results

Two-hundred and forty-four pharmacists responded to the survey. Of those respondents, 25% stated they had no knowledge about Florida’s drug importation plan. Less than 12% of respondents stated they would trust the safety and quality of imported medicines. Seventy percent of pharmacists expressed concerns regarding the changes required in pharmacy operations to increase medicine safety. About half of the respondents questioned whether this plan would promote cost-savings as intended.

Conclusion

Florida pharmacists believe the drug importation plan does not address all aspects of patient and medicine safety and expressed concerns regarding logistical operations of a pharmacy. This article highlights those concerns and acts as a summons to action.
The effect of inbreeding, body size and morphology on health in dog breeds
Authors: Bannasch Danika , Famula Thomas , Donner Jonas , Anderson Heidi , Honkanen Leena , Batcher Kevin , Safra Noa , Thomasy Sara , Rebhun Robert
Published: December 2021

Background

Dog breeds are known for their distinctive body shape, size, coat color, head type and behaviors, features that are relatively similar across members of a breed. Unfortunately, dog breeds are also characterized by distinct predispositions to disease. We explored the relationships between inbreeding, morphology and health using genotype based inbreeding estimates, body weight and insurance data for morbidity.

Results

The average inbreeding based on genotype across 227 breeds was Fadj = 0.249 (95% CI 0.235–0.263). There were significant differences in morbidity between breeds with low and high inbreeding (H = 16.49,P = 0.0004). There was also a significant difference in morbidity between brachycephalic breeds and non-brachycephalic breeds (P = 0.0048) and between functionally distinct groups of breeds (H = 14.95P < 0.0001). Morbidity was modeled using robust regression analysis and both body weight (P < 0.0001) and inbreeding (P = 0.013) were significant (r2 = 0.77). Smaller less inbred breeds were healthier than larger more inbred breeds.

Conclusions

In this study, body size and inbreeding along with deleterious morphologies contributed to increases in necessary health care in dogs.
High level of extra-pair paternity in the socially monogamous Marsh Tits (Poecile palustris)
Authors: Wang Juan , Wei Yanhui , Zhang Lei , Jiang Yiting , Li Keke , Wan Dongmei
Published: December 2021

Background

Extra-pair copulation behavior has been widely studied among socially monogamous birds. Many species revealed high rates of extra-pair paternity. But few of the studies have been carried out in the Asian population.

Methods

From 2012 to 2019, we explored the extra-pair paternity of Marsh Tits (Poecile palustris) in Xianrendong National Nature Reserve, Liaoning Province, China. During the study, adult Marsh Tits were captured with mist nets and parental birds, with nest-box traps. Blood samples were taken from the brachial vein. Parentage analyses were carried out using nine highly variable microsatellite loci through Cervus 3.0 software and maximum likelihood approach.

Results

Forty-nine offspring (15.08%) from 20 nests (45.45%) were the results of extra-pair fertilization out of a total of 325 offspring in 44 nests. The average extra-pair offspring ratio was 33.54%, with a set varying from 11.11 to 71.43%. Nine extra-pair fathers had been successfully identified, four of whom were the close neighbors of the focus nest while the nests of the remaining five were relatively far. No significant difference was found in the genetic similarity between the social and extra-pair mates of the female, nor in the heterozygosis among the maternal half-siblings.

Conclusions

In general, our study proved that the extra-pair paternity in Marsh Tits and its extra-pair mating is independent of the genetic compatibility hypothesis. This complements the understudied bird’s extra-pair paternity in Asian area and contributes to the comprehensive insight of birds’ extra-pair paternity behaviors.
Was Chagas disease responsible for Darwin’s illness? The overlooked eco-epidemiological context in Chile
Authors: Botto-Mahan Carezza , Medel Rodrigo
Published: December 2021

The source of Darwin’s illness has been a contentious issue in the literature for almost 70 years. Different causal factors have been invoked to account for his symptoms, including Chagas disease. The Chagas hypothesis is based upon Darwin’s diary, in which he narrates his experience with kissing bugs, the main vector of the protozoan Trypanosoma cruzi, the etiological agent of Chagas disease. In this contribution, we examine the consistency of the “Chagas disease hypothesis” in the light of current ecological and epidemiological knowledge of the disease in Chile. According to his diary and letters, during his overland trips, Darwin slept in rural houses and outdoors for 128 days in a “hyperendemic” area for Chagas disease, more than exposing him to kissing bugs. This observation conveys a likely additional source of infection than previously considered, which might reinforce the idea that Chagas disease contributed to Darwin’s manifest physical deterioration.
Analysis of childhood overweight and obesity in Namibia using spatio-temporal quantile interval models
Authors: Mtambo Owen Paweni Loss , Debusho Legesse Kassa
Published: December 2021

The global prevalence of overweight (including obesity) in children under 5 years of age was 7% in 2012, and it is expected to rise to 11% by the year 2025. The main objective of this study was to fit spatio-temporal quantile interval regression models for childhood overweight (including obesity) in Namibia from 2000 to 2013 using fully Bayesian inference implemented in R-INLA package in R version 3.5.1. All the available Demographic and Health Survey (DHS) datasets for Namibia since 2000 were used in this study. Significant determinants of childhood overweight (including obesity) ranged from socio-demographic factors to child and maternal factors. Child age and preceding birth interval had significant nonlinear effects on childhood overweight (including obesity). Furthermore, we observed significant spatial and temporal effects on childhood overweight (including obesity) in Namibia between 2000 and 2013. To achieve the World Health Organisation (WHO) global nutrition target 2025 in Namibia, the existing scaling-up nutrition programme and childhood malnutrition policy makers in this country may consider interventions based on socio-demographic determinants, and spatio-temporal variations presented in this paper.
Thinking global health from the perspective of anthropology
Authors: Ji Ruobing , Cheng Yu
Published: December 2021

COVID-19 has brought about political, economic, cultural, and interspecies problems far from medical areas, which challenges academia to rethink global health. For holism principle, anthropology offers valuable insights into these health issues, including the political economy of inequality, cultural diversity, and cultural adaptations, as well as the study of multispecies ethnography. These perspectives indicate that unequal political and economic systems contribute to health problems when people acknowledge disease and illness mechanisms. Moreover, cultural diversity and cultural adaptation are essential for providing appropriate medical solutions. Lastly, as a research method of studying interspecies relationships, multispecies ethnography promotes one health and planetary health from the ultimate perspective of holism. In conclusion, global health is not only a bio-medical concept but also involves political economy, culture, and multispecies factors, for which anthropology proffers inspiring theories and methods.
Characteristics of secondary droplets produced by the impact of drops onto a smooth surface
Authors: Zhang Haixiang , Gao Ye , Zhang Xiwen , Yi Xian , Du Yanxia , He Feng , Jin Zheyan , Hao Pengfei
Published: December 2021

This work investigates the splashing behaviors of droplets impacting on solid surfaces and mainly focuses on the characteristics of secondary droplets. According to the experimental results, two different splashing patterns, corona splash and levitating-lamella breakup, are observed. A new breakup mode, named rim-segmenting, is found during the levitating-lamella breakup. In particular, the detailed information of the splashing secondary droplets, including the size, velocity, angle, and total volume of the splashing secondary droplets is obtained from the experimental data. The size distribution of the splashing secondary droplets obeys the gamma distribution function. The average diameter and splashing angle of the secondary droplets are mainly related to the Reynolds number Re, and can be expressed as functions of Re. High impact velocity and liquid viscosity will result in a wider size distribution range of splashing secondary droplets. We also put forward an empirical model to predict the total splashing volume, which is consistent with the experimental data both in this work and previous studies. This work is believed to provide insights on the prediction of the characteristics of splashing secondary droplets.
Should we vaccinate the better seroconverters or the most vulnerable? Game changing insights for COVID-19 vaccine prioritization policies
Authors: AbdelMassih Antoine , El Shershaby Meryam , Gaber Hanya , Habib Menna , Gamal Nada , Husseiny Reem , AlShehry Nada , Amin Abeer , Heikal Bassant , El-Husseiny Nadine , Moursi Mai , Ismail Habiba-Allah , Senoussy Sara , ElSharkawy Reem , AlZayat Hebat Allah , ElMahdy Ghada , Moawad Hossameldin , Genena Ali , ElKiki Ahmed , Reda Mary , Khalil Mohamed , Al Ramady Reem , Radwan Noran , Khaled-Ibn-ElWalid Mariam , Amin Hager , Hozaien Rafeef , Kamel Aya , Fouda Raghda
Published: December 2021

Background

With the rapid rise in COVID 19 cases incomparable to the number of vaccinations available, there has been a demand to prioritize the older age groups receiving the vaccine as they have more risk of morbidity and mortality and thus better outcome from vaccination.

Main body

Some studies showed a lower seroconversion rate in older group patients; thus, we discuss the necessity to reprioritize vaccinations to younger age groups who have better seroconversion rates, but we may face some ethical dilemma that could hinder our hypothesis. Decreased seroconversion rates in adults are attributable to immuno-senescence which involves a decrease in humoral and cellular-mediated immunity with age. Despite this fact, there remains some ethical dilemma that can hinder widespread vaccination of younger generations, the most important of which is the unknown long-term effects of COVID-19 vaccines due their fast-tracking under the pressure of the pandemic.

Short conclusion

Prioritizing children vaccination against COVID-19 seems an interesting strategy that can help in containing the pandemic. Resolving some ethical dilemma needs to be done before implementing such strategy.
Omental cyst: a case report and review of the literature
Authors: Robbins Keenan J. , Antiel Ryan M. , Shakhsheer Baddr A.
Published: December 2021

Background

Omental cysts are rare, predominantly occur in children, and often initially present with symptoms masquerading as other more common intra-abdominal pathologies. In this case report, we present the case of a child with an omental cyst that originated from the lesser sac. Due to the location of this cyst, resection presented unique technical challenges that have not been described in existing literature.

Case presentation

A 4-year-old male patient presented with symptoms initially concerning for appendicitis. Ultrasound showed a normal appendix but a large volume of complex intraperitoneal fluid. Computed tomography subsequently demonstrated a large cystic structure spanning from the stomach to the bladder. The patient was taken to the operating room where a large omental cyst was found to originate from the lesser sac. The resection was difficult due to the thin wall of the cyst and the intimate association of the superior-most aspect of the cyst with the tail of the pancreas, but was ultimately successful.

Conclusions

Omental cysts are rarely suspected before detection on abdominal imaging. Surgical resection is the treatment of choice, and complete resection can result in a recurrence-free postoperative course. Laparoscopic resection has been reported, but laparotomy is reasonable when a minimally invasive approach may not allow for a safe resection without rupture of the cyst. Anatomical characteristics of the cyst, as demonstrated in our case, can present challenges in the treatment of this otherwise benign entity.