Effect of biofilm formation and cytotoxicity of biogenic silver nanoparticles (AgNPs) synthesized from a medicinal plant Sterculia foetida L.
Authors:   Department of Botany, St. Xavier’s College, (Affiliated to Manonmaniam Sundaranar University), Palayamkottai, Tirunelveli, Tamilnadu, India, Vasanth N. , Dons T. ,   Department of Botany, Sri Saradha College for Women, Perambalur, - 621113,Tamil Nadu, India, Michaelraj L. J. ,   Department of Botany, St. Xavier’s College, (Affiliated to Manonmaniam Sundaranar University), Palayamkottai, Tirunelveli, Tamilnadu, India, Ganesan P. ,   Interdisciplinary Research Centre in Biology, Xavier Research Foundation, St. Xavier’s College (ManonmaniamSundaranar University), Palayamkottai, Tirunelveli, Tamilnadu, India and The Anna and Donald Waite Chair, Creighton University, Omaha, USA, Salmen S. H. ,   Department of Botany and Microbiology, College of Science, King Saud University, PO Box -2455, Riyadh -11451, Saudi Arabia, Alharbi S. A. ,   Department of Botany and Microbiology, College of Science, King Saud University, PO Box -2455, Riyadh -11451, Saudi Arabia, Mutheeswaran S. ,   Interdisciplinary Research Centre in Biology, Xavier Research Foundation, St. Xavier’s College (ManonmaniamSundaranar University), Palayamkottai, Tirunelveli, Tamilnadu, India and The Anna and Donald Waite Chair, Creighton University, Omaha, USA, Anthonysamy M. ,   Department of Botany, St. Xavier’s College, (Affiliated to Manonmaniam Sundaranar University), Palayamkottai, Tirunelveli, Tamilnadu, India, Ignacimuthu S. ,   Interdisciplinary Research Centre in Biology, Xavier Research Foundation, St. Xavier’s College (ManonmaniamSundaranar University), Palayamkottai, Tirunelveli, Tamilnadu, India and The Anna and Donald Waite Chair, Creighton University, Omaha, USA, Stalin A. ,   State Key Laboratory of Subtropical Silviculture, Department of Traditional Chinese Medicine, Zhejiang A&F University, Hangzhou, China
Published: December 2023

The biogenic synthesis of silver nanoparticles mediated by medicinal plant Sterculia foetida was the prime experimental analysis of present study. The most important aim and focus of this study was to synthesis the AgNPs via biological method and the same had been evaluated against bio-film formation and dose dependent cyto-toxicity against cancer cells. This in vitro comprehensive analysis show that Ag had more advantage than other metals, the NPs was mediated by plant residue. And the NPs were further characterized by UV showing the sharp absorption peak at 455 nm; the identification of corresponding functional section proved by the parameter like FTIR, where conversion of Ag ions and capping agent is determined, the analysis on X- ray diffraction demonstrate the AgNPs found to be crystalline nature and face-centered like cubic structure. FESEM cum EDAX has showed the surface morphology with 40-50nm. After the physiochemical characterization, the AgNPs were evaluated with biofilm formation and cancer cells. In cyto-toxic study, two cell lines such as MCF 7 lung cancer cell line and A549 Breast cancer cell line were experimented and the values are AgNPs (IC50 =11.50± 0.05 µg and IC50 = 5.5± 0.05 µg/mL); AgNO3- (IC50 = 5.8± 0.05 µg and IC50 = 6.5± 0.05µg/mL) and Sterculia foetida (IC50 > 5000 µg/mL and IC50 < 5000 µg/mL). Another application of this present study is anti-biofilm assay. The selected bacterial strains are methicillinresistant Staphylococcus aureus; PA 14-Pseudomonas aeruginosa and Vibrio cholerae. Hence, the findings recommend that silver nanoparticles from medicinal plant Sterculia foetida is effective and can be used against bacteria and more precisely for cancer cell study.