bluuebunny/crossref_metadata_2025 huggingface dataset:
Doppler Lidar Investigations of Wind Turbine Near-Wakes and LES Modeling with New Porous Disc Approach
Authors: Uchida Takanori , Yoshida Tadasuke , Inui Masaki , Taniyama Yoshihiro
Published: April 2021

Many bottom-mounted offshore wind farms are currently planned for the coastal areas of Japan, in which wind speeds of 6.0–10.0 m/s are extremely common. The impact of such wind speeds is very relevant for the realization of bottom-mounted offshore wind farms. In evaluating the feasibility of these wind farms, therefore, strict evaluation at wind speeds of 6.0–10.0 m/s is important. In the present study, the airflow characteristics of 2 MW-class downwind wind turbine wake flows were first investigated using a vertically profiling remote sensing wind measurement device (lidar). The wind turbines used in this study are installed at the point where the sea is just in front of the wind turbines. A ground-based continuous-wave (CW) conically scanning wind lidar system (“ZephIR ZX300”) was used. Focusing on the wind turbine near-wakes, the detailed behaviors were considered. We found that the influence of the wind turbine wake, that is, the wake loss (wind velocity deficit), is extremely large in the wind speed range of 6.0–10.0 m/s, and that the wake loss was almost constant at such wind speeds (6.0–10.0 m/s). It was additionally shown that these results correspond to the distribution of the thrust coefficient of the wind turbine. We proposed a computational fluid dynamics (CFD) porous disk (PD) wake model as an intermediate method between engineering wake models and CFD wake models. Based on the above observations, the wind speed range for reproducing the behavior of the wind turbine wakes with the CFD PD wake model we developed was set to 6.0–10.0 m/s. Targeting the vertical wind speed distribution in the near-wake region acquired in the “ZephIR ZX300”, we tuned the parameters of the CFD PD wake model (CRC = 2.5). We found that in practice, when evaluating the mean wind velocity deficit due to wind turbine wakes, applying the CFD PD wake model in the wind turbine swept area was very effective. That is, the CFD PD wake model can reproduce the mean average wind speed distribution in the wind turbine swept area.
Transcriptomic Analysis of Seasonal Gene Expression and Regulation during Xylem Development in “Shanxin” Hybrid Poplar (Populus davidiana × Populus bolleana)
Authors: Zhao Chao , He Youchao , Yu Ying , Zhou Meiqi , Zhao Leifei , Xia Xiangyou , Wang Chao
Published: April 2021

Xylem development is a key process for wood formation in woody plants. To study the molecular regulatory mechanisms related to xylem development in hybrid poplar P. davidiana × P. bolleana, transcriptome analyses were conducted on developing xylem at six different growth stages within a single growing season. Xylem development and differentially expressed genes in the six time points were selected for a regulatory analysis. Xylem development was observed in stem sections at different growth stages, which showed that xylem development extended from the middle of April to early August and included cell expansion and secondary cell wall biosynthesis. An RNA-seq analysis of six samples with three replicates was performed. After transcriptome assembly and annotation, the differentially expressed genes (DEGs) were identified, and a Gene Ontology (GO) enrichment analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis and expression analysis of the DEGs were performed on each sample. On average, we obtained >20 million clean reads per sample, which were assembled into 84,733 nonredundant transcripts, of which there were 17,603 unigenes with lengths >1 kb. There were 14,890 genes that were differentially expressed among the six stages. The upregulated DEGs were enriched in GO terms related to cell wall biosynthesis between S1 vs. S2 or S3 vs. S4 and, in GO terms, related to phytohormones in the S1 vs. S2 or S4 vs. S5 comparisons. The downregulated DEGs were enriched in GO terms related to cell wall biosynthesis between S4 vs. S5 or S5 vs. S6 and, in GO terms, related to hormones between S1 vs. S2 or S2 vs. S3. The KEGG pathways in the DEGs related to “phenylpropanoid biosynthesis”, “plant hormone signal transduction” and “starch and sucrose metabolism” were significantly enriched among the different stages. The DEGs related to cell expansion, polysaccharide metabolism and synthesis, lignin synthesis, transcription factors and hormones were identified. The identification of genes involved in the regulation of xylem development will increase our understanding of the molecular regulation of wood formation in trees and, also, offers potential targets for genetic manipulation to improve the properties of wood.
Stand Stability of Pure and Mixed-Eucalyptus Forests of Different Tree Species in a Typhoon-Prone Area
Authors: Deng Haiyan , Shen Linlin , Yang Jiaqi , Mo Xiaoyong
Published: April 2021

Background and Objectives: The stable stand structure of mixed plantations is the basis of giving full play to forest ecological function and benefit. However, the monocultural Eucalyptus plantations with large-scale and successive planting that caused ecological problems such as reduced species diversity and loss of soil nutrients have presented to be unstable and vulnerable, especially in typhoon-prone areas. The objective of this study was to evaluate the nonspatial structure difference and the stand stability of pure and mixed-Eucalyptus forests, to find out the best mixed pattern of Eucalyptus forests with the most stability in typhoon-prone areas. Materials and Methods: In this study, we randomly investigated eight plots of 30 m × 30 m in pure and mixed-Eucalyptus (Eucalyptus urophylla S. T. Blake × E. grandis W. Hill) plantations of different tree species (Neolamarckia cadamba (Roxb.) Bosser, Acacia mangium Willd., and Pinus elliottii var. Elliottii Engelm. × P. caribaea Morelet) on growth status, characterized and compared the distribution of nonspatial structure of the monoculture and mixtures, and evaluated the stand quality and stability from eight indexes of the nonspatial structure, including preservation rate, stand density, height, diameter, stem form, degree of stem inclination, tree-species composition, and age structure. Results: Eucalyptus surviving in the mixed plantation of Eucalyptus and A. mangium (EA) and in the mixed plantation of Eucalyptus and P. elliottii × P. caribaea (EP) were 5.0% and 7.6% greater than those in pure Eucalyptus plantation (EE), respectively, while only the stand preservation rate of EA was greater (+2.9%) than that of the pure Eucalyptus plantation. The proportions of all mixtures in the height class greater than 7 m were fewer than that of EE. The proportions of EA and mixed plantation of Eucalyptus and N. cadamba (EN) in the diameter class greater than 7 m were 10.6% and 7.8%, respectively, more than that of EE. EN had the highest ratio of branching visibly (41.0%), EA had the highest ratio of inclined stems (8.1%), and EP had the most straight and complete stem form (68.7%). The stand stability of the mixed plantation of Eucalyptus and A. mangium presented to be optimal, as its subordinate function value (0.76) and state value (ω = 0.61) of real stand were the largest. Conclusions: A. mangium is a superior tree species to mix with Eucalyptus for a more stable stand structure in the early growth stage to approach an evident and immense stability and resistance, which is of great significance for the forest restoration of Eucalyptus in response to extreme climate and forest management.
Wave-Energy Dissipation: Seaweeds and Marine Plants Are Ecosystem Engineers
Authors: Denny Mark
Published: April 2021

Ocean waves deliver an immense amount of energy to coasts around the planet, powering high-velocity flows that interact with nearshore marine plants and animals. Although some of these interactions are beneficial, it is often advantageous for subtidal and intertidal ecological communities if wave-induced water velocities can be reduced by safely dissipating wave energy. This function is often fulfilled by seaweeds and marine plants, which thereby act as ecosystem engineers, modifying the environment to the benefit of the community. Recent advances in hydro-mechanical theory help to explain the mechanisms by which vegetation dissipates wave energy, highlighting the role that organisms’ tendency to bend in flow—their structural flexibility—plays in their ability to engineer wave-induced flows. Here, I review these theories and their application to salt marsh plants, seagrasses, mangroves, and seaweeds, focusing on the ways that marine vegetation serves a foundational role in community function.
School Choice in Guangzhou: Why High-Scoring Students Are Protected?
Authors: Fang Yuanju
Published: April 2021

Each year, millions of middle school graduates in China take a standardized test and compete for high school positions. Unlike other cities, Guangzhou still uses the immediate acceptance mechanism but implements a policy that students in the high-scoring group receive their allocations before those in the low-scoring group. In this paper, we study a class of the Guangzhou mechanisms, including the immediate acceptance (IA) and the serial dictatorship (SD) mechanism. We show that, if a collection of groups is refined by splitting its groups into a larger number of smaller subgroups, then the Guangzhou mechanism will perform more stably and less manipulable than before. This result provides a tool for policy makers to improve the allocation outcome of the IA mechanism under homogeneous priorities and justifies the use of a high-scoring student protection policy in Guangzhou’s high school admission.
Stress Salivary Biomarkers Variation during the Work Day in Emergencies in Healthcare Professionals
Authors: Pérez-Valdecantos Daniel , Caballero-García Alberto , Del Castillo-Sanz Teodosia , Bello Hugo J. , Roche Enrique , Córdova Alfredo
Published: April 2021

Objective: The work of health professionals in hospital emergency rooms is highly demanding due to the decisions they must take. In the present study, we consider assessing stress response in emergency health workers, measuring related biomarkers such as cortisol, dehydroepiandrosterone (DHEA) and salivary α-amylase during the whole working day. Method: An analytical, descriptive and cross-sectional study was carried out. The study was conducted in the emergency rooms of two public hospitals. Ninety-seven professionals participated, 45 corresponding to one hospital and 52 to the other. Four salivary samples were obtained according to circadian rhythms: at 8:00, 12:00, 15:00 and 00:00 h/24 h. The data were subsequently analyzed. Results: Cortisol levels decreased throughout the working day, with minimum values being at 24 h. A similar pattern was observed in DHEA. The α-amylase levels increased throughout the working day, reaching its peak at 15:00 h, and decreasing at 24 h, compared to the data from the rest of the working day. Conclusions: Since reference/baseline values are not presented, this work is focused on a stress situation experienced during one regular working day in emergency rooms with no extreme situations. In this context, stress, measured through cortisol and α-amylase, is present in emergency room doctors and nurses. However, the increase in DHEA, due to its anabolic condition, could counteract their effect, suggesting a positive effect on their professional actions.
Neonatal End-of-Life Decision Making: The Possible Behavior of Greek Physicians, Midwives, and Nurses in Clinical Scenarios
Authors: Dagla Maria , Petousi Vasiliki , Poulios Antonios
Published: April 2021

Background: This study investigates the acceptability, bioethical justification, and determinants of the provision of intensive care to extremely preterm or ill neonates among healthcare professionals serving in NICUs in Greek hospitals. Methods: Healthcare professionals (71 physicians, 98 midwives, and 82 nurses) employed full-time at all public Neonatal Intensive Care Units (NICUs) (n = 17) in Greece were asked to report their potential behavior in three clinical scenarios. Results: The majority of healthcare professionals would start and continue intensive care to (a) an extremely preterm neonate, (b) a full-term neonate with an unfavorable prognosis, and (c) a neonate with complete phocomelia. In cases (a) and (b), midwives and nurses compared to physicians (p = 0.009 and p = 0.004 in scenarios (a) and (b), respectively) and health professionals ascribing to the quality-of-life principle compared to those ascribing to the intrinsic value of life (p = 0.001 and p = 0.01 scenarios (a) and (b) respectively), tend towards withholding or withdrawing care. Religion plays an important role in all three scenarios (p = 0.005, p = 0.017 and p = 0.043, respectively). Conclusions: Understanding healthcare professionals’ therapeutic intensiveness in the face of NICU ethical dilemmas can improve NICU policies, support strategies, and, consequently, the quality of neonatal intensive care.
Water Ski Injuries and Chronic Pain in Collegiate Athletes
Authors: Jung Hyun Chul , Straltsova Hanna , Woodgate Michael A. , Kim Kyung-Min , Lee Jung-Min , Lee Joon-Hee , Gann Joshua J.
Published: April 2021

This study examined the rate of injuries and chronic pain in collegiate water-ski athletes as a preliminary study. We also compared the mechanics and cause of injuries by the level of water-skiing experiences. A total number of 96 collegiate water-ski athletes, aged 21.4 ± 2.23 years, participated in the study. An off-line questionnaire was distributed at the collegiate tournaments in the United States. The questionnaire consisted of 20 questions, including demographic information, body region and type of injuries, mechanics and cause of injuries, chronic pain and pain management. A Chi-squared test was used to examine the differences in injury rates by sex and the level of experiences (beginner: <5 years, intermediate: 5–10 years, advanced: <10 years). The significance level was set at ≤0.05. A total of 336 water skiing-related injuries were observed from 96 participants. The ankle/feet, knee, and head/neck regions were the most common body regions injured, representing 26.5, 16.7, and 15.8%, respectively. Female athletes were more likely to have nerve injuries than male athletes (p = 0.039). The intermediate athletes were more likely to have trunk (p = 0.047) and upper extremity (p = 0.042) injuries than beginner athletes, and the beginner athletes had less joint/ligament (p = 0.001) and bone injury (p = 0.010) compared to the advanced athletes. Torsion/twisting (32.8%) and deceleration (26.9%) were the most common mechanism of injury. Beginner athletes experienced injuries more due to insufficient skill (p = 0.03), while the advanced athletes were likely to have more injuries by the loss of control (p = 0.01). Collegiate athletes had higher rates of chronic pain in the trunk (42.7%) and skeletal muscle (43.8%), and they participated in stretching/exercise (40.8%) and massage/form rolling (29.6%) to manage their chronic pain. The present study revealed that injury rates in males and females were 49.7% and 50.2%, respectively. Female athletes were more likely to have a nerve injury than male athletes. The mechanics and cause of injuries were different by the level of experiences where different training approaches may be required to minimize the injuries. Additionally, the strength and conditioning program that is systematically designed for core strength is needed to eliminate chronic trunk pain in collegiate water-skiing athletes.
Perspectives from Dentists, Dental Assistants, Students, and Patients on Dental Care Adapted to the COVID-19 Pandemic: A Cross-Sectional Survey
Authors: Schlenz Maximiliane Amelie , Schmidt Alexander , Wöstmann Bernd , May Andreas , Howaldt Hans-Peter , Albert Dennis , Ziedorn Doreen , Krämer Norbert , Schulz-Weidner Nelly
Published: April 2021

Dental care has been affected by SARS-CoV-2 (COVID-19) worldwide. In contrast to other dental clinics, the Justus-Liebig-University Giessen (Germany) decided not to limit dental treatment to emergencies alone, but to continue dental care for all patients, with increased safety measures. As such, health care professionals may be exposed to additional physical and mental stress. The aim of this study was to assess the perspectives of all persons involved in dental care (dentists, dental assistants, students, and patients) regarding the aspects of safety measures, anxiety about self-infection and infecting others, and other prospects in the period March to December 2020 using a questionnaire. Data collection was performed between 14 December 2020 and 23 January 2021. A total of 35 dentists (response rate of 79.5%), 23 dental assistants (65.7%), 84 students (80%), and 51 patients (21.8%) completed the survey. The patients did not notice any changes in the care received. Dentists and dental assistants reported a higher workload due to additional safety measures. The majority of dentists, students, and patients agreed that normal patient care was maintained. One-third of dental assistants would have preferred emergency treatment alone and expressed significantly higher anxiety about COVID-19 infection than all other groups (p < 0.05). In conclusion, all groups showed a predominantly positive perspective on dental care, and anxiety about self-infection and infecting others was especially low. However, additional measures are time-consuming and compound daily patient care. This concept, based on well-established infection control, might be a viable proposal for current and future pandemics.
Resettlement Stressors for Women of Refugee Background Resettled in Regional Australia
Authors: Hawkes Clare , Norris Kimberley , Joyce Janine , Paton Douglas
Published: April 2021

Women of Refugee Background (WoRB) have been repeatedly identified as an extremely vulnerable population. Within an Australian context, WoRB are increasingly resettled to non-metropolitan locations, otherwise known as regional locations. Despite this, to date, no research has focused on the lived experience and challenges associated with the resettlement of WoRB to regional contexts. This study aimed to address this gap in the literature by investigating the resettlement experience of WoRB resettled in Tasmania—a state in Australia classified as a rural and regional location. Qualitative interviews were conducted with a group of 21 individuals (nine WoRB and 12 service providers). Thematic analysis identified four overarching themes—Communication Barriers and Lack of Fluency in English, Challenges Accessing Everyday Basic Needs, Loss of Connection to Culture of Origin and Inability to Access Mainstream Mental Health Services for Help. Participants also highlighted a number of unique gender-related vulnerabilities experienced during resettlement, which were exacerbated in regional locations due to health services being overstretched and under-resourced. Results of the current study are discussed in regard to policy and practical implications, taking into consideration the unique vulnerabilities experienced by WoRB, which, to date, are often overlooked.
Earlier Is Better? Timing of Adductor Canal Block for Arthroscopic Knee Surgery under General Anesthesia: A Retrospective Cohort Study
Authors: Wu Shao-Chun , Hsu Chih-Yi , Lu Hsiao-Feng , Chen Chih-Chun , Hou Shao-Yun , Poon Yan-Yuen
Published: April 2021

The adductor canal block (ACB) is effective for treating postoperative pain during arthroscopic knee surgery, but its impact on anesthesia course and the optimal administration timing are unknown. This retrospective study addressed these questions. The aim of this study was to compare the effects of preoperative ACB and postoperative ACB on anesthesia course and postoperative recovery. We allocated 215 adult patients who underwent arthroscopic knee surgery under sevoflurane anesthesia between January 2019 and December 2019 to three groups. Group A received general anesthesia without ACB, Group B received ACB before general anesthesia induction, and Group C received ACB in the post-anesthesia recovery unit (PACU). Group B consumed significantly less sevoflurane (0.19 mL/kg/h) and milligram morphine equivalents (0.08 MME) intraoperatively than Groups A (0.22 mL/kg/h; 0.10 MME, respectively) and C (0.22 mL/kg/h; 0.09 MME, respectively). Groups B and C had lower visual analogue scale (VAS) scores upon PACU discharge than Group A. Dynamic, but not at-rest VAS scores, were significantly higher in Group A. Opioid consumption was similar in the ward, but Group A requested more intravenous parecoxib for pain relief. Length of hospital stay was similar. Thus, preoperative ACB reduced the amount of volatile anesthetic required and maintained stable hemodynamics intraoperatively. Preoperative or postoperative ACB improved postoperative pain control. Consequently, preoperative ACB is optimal for intraoperative stress suppression and postoperative pain control.
Relationships between College Students’ Sedentary Behavior, Sleep Quality, and Body Mass Index
Authors: Liu Wenxi , Yuan Qin , Zeng Nan , McDonough Daniel J. , Tao Kun , Peng Qingwen , Gao Zan
Published: April 2021

Purpose: Sedentary behavior (SB), sleep efficiency (SE), sleep duration (SD), and body mass index (BMI) are crucial determinants of an individual’s health. However, empirical evidence regarding associations between these factors in young adults living in China remains unknown. Therefore, the purpose of this study was to examine the relationships between accelerometer-measured SB, SE, SD, and BMI in Chinese college students. Methods: Two-hundred and twenty college students (115 females, Meanage = 20.29 years, SD = 2.37) were recruited from a south-central Chinese university. Participants’ SB (daily % time spent in SB), SE (number of minutes of sleep duration/number of minutes in bed), and SD were assessed via wrist-worn ActiGraph GT9X Link accelerometers for one week. Body weight was measured using a digital weight scale, height was measured using a stadiometer, and BMI was calculated as weight (kg)/height (m2). Results: Participants’ average time spent in SB was 76.52% (SD = 10.03), SE was 84.12% (SD = 4.79), and BMI was 20.67 kg/m2 (SD = 3.12), respectively. Regression analyses indicated that SB (β = −0.17, p = 0.01) and BMI (β = −0.20, p < 0.01) negatively predicted SE. In addition, BMI negatively predicted SD (β = −0.22, p < 0.01). Conclusion: Prolonged SB (e.g., screen viewing, smartphone use, and computer playing) and higher BMI may link to shorter sleep duration and lower sleep efficiency in Chinese young adults. Future randomized controlled trials are needed to further confirm these findings. Given that increased BMI status and SB may relate to adverse health outcomes, more population-based intervention strategies seeking to lower BMI and reduce SB (e.g., nutrition education and physical activity promotion) are needed in this population.
Framing Environmental Health Decision-Making: The Struggle over Cumulative Impacts Policy
Authors: Payne-Sturges Devon C. , Sangaramoorthy Thurka , Mittmann Helen
Published: April 2021

Little progress has been made to advance U.S. federal policy responses to growing scientific findings about cumulative environmental health impacts and risks, which also show that many low income and racial and ethnic minority populations bear a disproportionate share of multiple environmental burdens. Recent scholarship points to a “standard narrative” by which policy makers rationalize their slow efforts on environmental justice because of perceived lack of data and analytical tools. Using a social constructivist approach, ethnographic research methods, and content analysis, we examined the social context of policy challenges related to cumulative risks and impacts in the state of Maryland between 2014 and 2016. We identified three frames about cumulative impacts as a health issue through which conflicts over such policy reforms materialize and are sustained: (a) perceptions of evidence, (b) interpretations of social justice, and (c) expectations of authoritative bodies. Our findings illustrate that policy impasse over cumulative impacts is highly dependent on how policy-relevant actors come to frame issues around legislating cumulative impacts, rather than the “standard narrative” of external constraints. Frame analysis may provide us with more robust understandings of policy processes to address cumulative risks and impacts and the social forces that create health policy change.
Consumer Choices and Habits Related to Coffee Consumption by Poles
Authors: Czarniecka-Skubina Ewa , Pielak Marlena , Sałek Piotr , Korzeniowska-Ginter Renata , Owczarek Tomasz
Published: April 2021

Coffee is one of the most popular drinks consumed in the world, also in Poland. In the literature, much attention is paid to the influence of coffee on human health, especially daily intake of caffeine, and also purchasing consumer behavior. There is a lack of research devoted to consumer choices and habits in relation to coffee consumption and brewing method. Therefore, the aim of this study is to describe the characteristics of coffee consumers and present their segmentation based on consumer choices and habits towards coffee consumption. The study was performed using the computer-assisted web interviewing (CAWI) method on a group of 1500 adults respondents in Poland reporting the consumption of coffee. We collected information about consumer choices and habits related to coffee consumption, including brewing method, place of consuming coffee, and factors determining coffee choices. Using cluster analysis, we identified three main groups of coffee consumers. There are “Neutral coffee drinkers”, “Ad hoc coffee drinkers”, and “Non-specific coffee drinkers”. The respondents in the study are not coffee gourmets; they like and consume coffee, but these are often changing choices. To conclude, it can be stated that the Polish coffee consumer prefers conventional methods of brewing coffee (like a “traditionalist”) but is open to novelties and new sensory experiences. Based on study results it is possible to know the coffee drinking habits in Poland.
A Scoping Review of Homebound Older People: Definition, Measurement and Determinants
Authors: Ko Young , Noh Wonjung
Published: April 2021

Being homebound (HB) can affect people’s physical and mental health by decreasing movement, which can itself be exacerbated by the deterioration of people’s health. To break this vicious cycle of HB and being in poor health, it is necessary to identify and address the factors influencing HB status. Thus, we used a scoping review to identify an HB trend, focusing on the definition, measurements, and determinants of HB status. We analyzed 47 studies according to the five-stage methodological framework for scoping reviews. The common attribute of definitions of HB status was that the boundaries of daily life are limited to the home. However, this varied according to duration and causes of becoming HB; thus, the understanding of HB shifted from the presence or absence of being HB to the continuum of daily activity. Various definitions and measurements have been used to date. Many studies have focused on individual factors to analyze the effect of HB. In the future, it will be necessary to develop a standardized measurement that reflects the multidimensional HB state. In addition, it is necessary to utilize a theoretical framework to explore the social and environmental factors affecting HB.
Street Food Stand Availability, Density, and Distribution Across Income Levels in Mexico City
Authors: Rosales Chavez Jose B. , Bruening Meg , Ohri-Vachaspati Punam , Lee Rebecca E. , Jehn Megan
Published: April 2021

Street food stands (SFS) are an understudied element of the food environment. Previous SFS studies have not used a rigorous approach to document the availability, density, and distribution of SFS across neighborhood income levels and points of access in Mexico City. A random sample (n = 761) of street segments representing 20 low-, middle-, and high-income neighborhoods were assessed using geographic information system (GIS) and ground-truthing methods. All three income levels contained SFS. However, SFS availability and density were higher in middle-income neighborhoods. The distribution of SFS showed that SFS were most often found near homes, transportation centers, and worksites. SFS availability near schools may have been limited by local school policies. Additional studies are needed to further document relationships between SFS availability, density, and distribution, and current structures and processes.
Long Term Memory Outcome of Repetitive, Low-Level Dietary Exposure to Domoic Acid in Native Americans
Authors: Grattan Lynn M. , Kaddis Laura , Tracy J. Kate , Morris John Glenn
Published: April 2021

Domoic acid (DA) is a marine-based neurotoxin that, if ingested via tainted shellfish, is associated with Amnesic Shellfish Poisoning (ASP). These acute effects of elevated DA exposure in humans have been well described. In contrast, the long-term impacts of lower level, repetitive, presumably safe doses of DA (less than 20 ppm) are minimally known. Since Native Americans (NA) residing in coastal communities of the Pacific NW United States are particularly vulnerable to DA exposure, this study focuses on the long-term, 8-year memory outcome associated with their repeated dietary consumption of the neurotoxin. Measures of razor clam consumption, memory, clerical speed and accuracy, and depression were administered over eight years to 500 randomly selected adult NA men and women ages 18–64. Data were analyzed using GEE analyses taking into consideration the year of study, demographic factors, and instrumentation in examining the association between dietary exposure and outcomes. Findings indicated a significant but small decline in total recall memory within the context of otherwise stable clerical speed and accuracy and depression scores. There is reason to believe that a continuum of memory difficulties may be associated with DA exposure, rather than a unitary ASP syndrome.
Sex Worker Health Outcomes in High-Income Countries of Varied Regulatory Environments: A Systematic Review
Authors: McCann Jessica , Crawford Gemma , Hallett Jonathan
Published: April 2021

There is significant debate regarding the regulation of the sex industry, with a complex range of cultural, political and social factors influencing regulatory models which vary considerably between and within countries. This systematic review examined the available evidence on the relationship between different approaches to sex industry regulation in high-income countries, and associated effects on sex worker health status. Objectives included identification of sex worker health outcomes, including sexual health, substance use and experience of stigma and violence. A search was performed electronically in eight scholarly databases which yielded 95 articles which met the criteria for inclusion. Findings suggested that sex workers in legalised and decriminalized countries demonstrated greater health outcomes, including awareness of health conditions and risk factors.
Public KAP towards COVID-19 and Antibiotics Resistance: A Malaysian Survey of Knowledge and Awareness
Authors: Chang Chee Tao , Lee Ming , Lee Jason Choong Yin , Lee Nicholas Chor Teng , Ng Tsu Yin , Shafie Asrul Akmal , Thong Kah Shuen
Published: April 2021

This study aimed to assess the knowledge of the Malaysian public on the coronavirus disease 2019 (COVID-19) and antibiotics, the practice of preventive measures and attitude towards the new norms. The web-based questionnaire was disseminated online from 1 to 31 October 2020. Out of 2117 respondents, 1405 (66.4%) knew that transmission of COVID-19 virus could happen in asymptomatic people. In term of antibiotics knowledge, 779 (36.8%) respondents were aware that taking antibiotics could not speed up the recovery process of all infections. Less than half of the respondents (49.0%) knew that antibiotics are effective against bacterial infection only. Majority (92.3%) practiced good preventive measures. Majority of the respondents strongly agreed that quarantine should be made mandatory for all arrival from overseas (97.2%) and wearing face masks should be made mandatory in all public areas (94.0%). Respondents of Chinese ethnicity (p = 0.008), middle-aged (p = 0.002), with tertiary education (p = 0.015) and healthcare related education (p < 0.001), from the higher income groups (p = 0.001) were more likely to have better knowledge on COVID-19. The Malaysian public demonstrated good knowledge towards COVID-19, adequate practice of preventive measures and high acceptance towards the new norm. Knowledge on antibiotics use and resistance was poor, which warrants attention from the health authorities.
Burnout and Its Relationship with Demographic and Job-Related Variables among Dentists in Lithuania: A Cross-Sectional Study
Authors: Slabšinskienė Eglė , Gorelik Andrej , Kavaliauskienė Aistė , Zaborskis Apolinaras
Published: April 2021

Although burnout has been described as a serious hazard for personal and professional lives and has been surveyed among dentists in many countries, no study has been published regarding burnout among dentists in Lithuania. This study aimed to evaluate the burnout level among Lithuanian dentists and its association with demographic variables, job satisfaction, and other job-related variables. The data were collected among dentists online or during professional conferences while using an anonymous questionnaire (n = 380). The Maslach Burnout Inventory (MBI) was used to evaluate the burnout level. A Poisson regression was applied for the analysis of relationships between variables. We observed that 42.3% of the respondents had a high emotional exhaustion (EE) (95% confidence interval (CI): 37.4–42.3%), while 18.7% (95% CI: 15.0–22.9%) and 28,2% (95% CI: 23.4–32.6%) had high depersonalization (DP) and low personal accomplishment (PA), respectively. Nonetheless, 15.3% (95% CI: 11.8–18.9%) of the study population experienced a high level of overall burnout. An original job satisfaction index was elaborated. It was significantly associated with sum scores of all burnout dimensions: with the EE sum score (Ratio of Sum Score Means (RSSM) 1.54; 95% CI: 1.46–1.62), DP sum score (RSSM 1.59; 95% CI: 1.45–1.74), and PA sum score (RSSM 0.88; 95% CI: 0.84–0.92). It was concluded that Lithuanian dentists can be characterised by high burnout intensity and high prevalence of burnout, being especially evident in emotional exhaustion. The dentist with low job satisfaction appeared to be the most vulnerable to all burnout dimensions.
The Relationship between Change of Weight and Chronic Low Back Pain in Population over 50 Years of Age: A Nationwide Cross-Sectional Study
Authors: Lee Choung Ah , Jang Hae-Dong , Moon Ji Eun , Han Sangsoo
Published: April 2021

Introduction: There is increasing evidence supporting an association between obesity and low back pain (LBP). However, the association between weight change and LBP in the general population is poorly understood. We investigated the relationship between weight change and LBP in a representative sample of the Korean general population from a nationwide survey. Methods: We analyzed data collected from the Korea National Health and Nutrition Examination Survey VI (2013–2015). Chronic LBP was defined as LBP lasting over 30 days in the last 3 months in the self-report health survey. Weight change was defined as the difference in weight from one year prior, and the amount of change was divided into no change, 3–6 kg, and ≥6 kg. Sampling weights were used to generate representative estimates for the general Korean population. Results: Overall, 6629 (12.0%) and 1848 (11.5%) participants were in the non-LBP and LBP groups, respectively. On multiple regression analysis, weight gain was significantly associated with LBP (adjusted odds ratio (OR) 1.29, p = 0.011), compared with no weight change. Weight gain of ≥6 kg was particularly closely associated with LBP (adjusted OR 1.42, p = 0.037), compared with no weight change. No association was found between LBP and weight loss. Conclusion: Weight gain is significantly associated with chronic LBP and, in particular, the greater the amount of weight gain, the stronger the association with an increased risk of chronic LBP. Clinicians should carefully monitor weight gain in LBP patients.
A Low-Cost Assistive Robot for Children with Neurodevelopmental Disorders to Aid in Daily Living Activities
Authors: López-Sastre Roberto J. , Baptista-Ríos Marcos , Acevedo-Rodríguez Francisco Javier , Pacheco-da-Costa Soraya , Maldonado-Bascón Saturnino , Lafuente-Arroyo Sergio
Published: April 2021

In this paper, we present a new low-cost robotic platform that has been explicitly developed to increase children with neurodevelopmental disorders’ involvement in the environment during everyday living activities. In order to support the children and youth with both the sequencing and learning of everyday living tasks, our robotic platform incorporates a sophisticated online action detection module that is capable of monitoring the acts performed by users. We explain all the technical details that allow many applications to be introduced to support individuals with functional diversity. We present this work as a proof of concept, which will enable an assessment of the impact that the developed technology may have on the collective of children and youth with neurodevelopmental disorders in the near future.
The Effects of 15 or 30 s SIT in Normobaric Hypoxia on Aerobic, Anaerobic Performance and Critical Power
Authors: Karabiyik Hakan , Eser Mustafa Can , Guler Ozkan , Yasli Burak Caglar , Ertetik Goktug , Sisman Aysegul , Koz Mitat , Gabrys Tomasz , Pilis Karol , Karayigit Raci
Published: April 2021

Sprint interval training (SIT) is a concept that has been shown to enhance aerobic-anaerobic training adaptations and induce larger effects in hypoxia. The purpose of this study was to examine the effects of 4 weeks of SIT with 15 or 30 s in hypoxia on aerobic, anaerobic performance and critical power (CP). A total of 32 male team players were divided into four groups: SIT with 15 s at FiO2: 0.209 (15 N); FiO2: 0.135 (15 H); SIT with 30 s at FiO2: 0.209 (30 N); and FiO2: 0.135 (30 H). VO2max did not significantly increase, however time-to-exhaustion (TTE) was found to be significantly longer in the post test compared to pre test (p = 0.001) with no difference between groups (p = 0.86). Mean power (MPw.kg) after repeated wingate tests was significantly higher compared to pre training in all groups (p = 0.001) with no difference between groups (p = 0.66). Similarly, CP was increased in all groups with 4 weeks of SIT (p = 0.001) with no difference between groups (p = 0.82). This study showed that 4 weeks of SIT with 15 and 30 s sprint bouts in normoxia or hypoxia did not increased VO2max in trained athletes. However, anerobic performance and CP can be increased with 4 weeks of SIT both in normoxia or hypoxia with 15 or 30 s of sprint durations.
The Critical Role of NLRP6 Inflammasome in Streptococcus pneumoniae Infection In Vitro and In Vivo
Authors: Xu Dongyi , Wu Xingping , Peng Lianci , Chen Tingting , Huang Qingyuan , Wang Yu , Ye Chao , Peng Yuanyi , Hu Dongliang , Fang Rendong
Published: April 2021

Streptococcus pneumoniae (S. pneumoniae) causes severe pulmonary diseases, leading to high morbidity and mortality. It has been reported that inflammasomes such as NLR family pyrin domain containing 3 (NLRP3) and absent in melanoma 2 (AIM2) play an important role in the host defense against S. pneumoniae infection. However, the role of NLRP6 in vivo and in vitro against S. pneumoniae remains unclear. Therefore, we investigated the role of NLRP6 in regulating the S. pneumoniae-induced inflammatory signaling pathway in vitro and the role of NLRP6 in the host defense against S. pneumoniae in vivo by using NLRP6−/− mice. The results showed that the NLRP6 inflammasome regulated the maturation and secretion of IL-1β, but it did not affect the induction of IL-1β transcription in S. pneumoniae-infected macrophages. Furthermore, the activation of caspase-1, caspase-11, and gasdermin D (GSDMD) as well as the oligomerization of apoptosis-associated speck-like protein (ASC) were also mediated by NLRP6 in S. pneumoniae-infected macrophages. However, the activation of NLRP6 reduced the expression of NF-κB and ERK signaling pathways in S. pneumoniae-infected macrophages. In vivo study showed that NLRP6−/− mice had a higher survival rate, lower number of bacteria, and milder inflammatory response in the lung compared with wild-type (WT) mice during S. pneumoniae infection, indicating that NLRP6 plays a negative role in the host defense against S. pneumoniae. Furthermore, increased bacterial clearance in NLRP6 deficient mice was modulated by the recruitment of macrophages and neutrophils. Our study provides a new insight on S. pneumoniae-induced activation of NLRP6 and suggests that blocking NLRP6 could be considered as a potential therapeutic strategy to treat S. pneumoniae infection.
JNK Pathway in CNS Pathologies
Authors: de los Reyes Corrales Teresa , Losada-Pérez María , Casas-Tintó Sergio
Published: April 2021

The c-Jun N-terminal kinase (JNK) signalling pathway is a conserved response to a wide range of internal and external cellular stress signals. Beside the stress response, the JNK pathway is involved in a series of vital regulatory mechanisms during development and adulthood that are critical to maintain tissue homeostasis. These mechanisms include the regulation of apoptosis, growth, proliferation, differentiation, migration and invasion. The JNK pathway has a diverse functionality and cell-tissue specificity, and has emerged as a key player in regeneration, tumorigenesis and other pathologies. The JNK pathway is highly active in the central nervous system (CNS), and plays a central role when cells need to cope with pathophysiological insults during development and adulthood. Here, we review the implications of the JNK pathway in pathologies of the CNS. More specifically, we discuss some newly identified examples and mechanisms of JNK-driven tumor progression in glioblastoma, regeneration/repair after an injury, neurodegeneration and neuronal cell death. All these new discoveries support the central role of JNK in CNS pathologies and reinforce the idea of JNK as potential target to reduce their detrimental effects.
Genetic Screen in Adult Drosophila Reveals That dCBP Depletion in Glial Cells Mitigates Huntington Disease Pathology through a Foxo-Dependent Pathway
Authors: Martin Elodie , Heidari Raheleh , Monnier Véronique , Tricoire Hervé
Published: April 2021

Huntington’s disease (HD) is a progressive and fatal autosomal dominant neurodegenerative disease caused by a CAG repeat expansion in the first exon of the huntingtin gene (HTT). In spite of considerable efforts, there is currently no treatment to stop or delay the disease. Although HTT is expressed ubiquitously, most of our knowledge has been obtained on neurons. More recently, the impact of mutant huntingtin (mHTT) on other cell types, including glial cells, has received growing interest. It is currently unclear whether new pathological pathways could be identified in these cells compared to neurons. To address this question, we performed an in vivo screen for modifiers of mutant huntingtin (HTT-548-128Q) induced pathology in Drosophila adult glial cells and identified several putative therapeutic targets. Among them, we discovered that partial nej/dCBP depletion in these cells was protective, as revealed by strongly increased lifespan and restored locomotor activity. Thus, dCBP promotes the HD pathology in glial cells, in contrast to previous opposite findings in neurons. Further investigations implicated the transcriptional activator Foxo as a critical downstream player in this glial protective pathway. Our data suggest that combinatorial approaches combined to specific tissue targeting may be required to uncover efficient therapies in HD.
Overview of the Therapeutic Potential of Aptamers Targeting Coagulation Factors
Authors: Liu Max , Zaman Khalequz , Fortenberry Yolanda M.
Published: April 2021

Aptamers are single-stranded DNA or RNA sequences that bind target molecules with high specificity and affinity. Aptamers exhibit several notable advantages over protein-based therapeutics. Aptamers are non-immunogenic, easier to synthesize and modify, and can bind targets with greater affinity. Due to these benefits, aptamers are considered a promising therapeutic candidate to treat various conditions, including hematological disorders and cancer. An active area of research involves developing aptamers to target blood coagulation factors. These aptamers have the potential to treat cardiovascular diseases, blood disorders, and cancers. Although no aptamers targeting blood coagulation factors have been approved for clinical use, several aptamers have been evaluated in clinical trials and many more have demonstrated encouraging preclinical results. This review summarized our knowledge of the aptamers targeting proteins involved in coagulation, anticoagulation, fibrinolysis, their extensive applications as therapeutics and diagnostics tools, and the challenges they face for advancing to clinical use.
Mitophagy in Human Diseases
Authors: Doblado Laura , Lueck Claudia , Rey Claudia , Samhan-Arias Alejandro K. , Prieto Ignacio , Stacchiotti Alessandra , Monsalve Maria
Published: April 2021

Mitophagy is a selective autophagic process, essential for cellular homeostasis, that eliminates dysfunctional mitochondria. Activated by inner membrane depolarization, it plays an important role during development and is fundamental in highly differentiated post-mitotic cells that are highly dependent on aerobic metabolism, such as neurons, muscle cells, and hepatocytes. Both defective and excessive mitophagy have been proposed to contribute to age-related neurodegenerative diseases, such as Parkinson’s and Alzheimer’s diseases, metabolic diseases, vascular complications of diabetes, myocardial injury, muscle dystrophy, and liver disease, among others. Pharmacological or dietary interventions that restore mitophagy homeostasis and facilitate the elimination of irreversibly damaged mitochondria, thus, could serve as potential therapies in several chronic diseases. However, despite extraordinary advances in this field, mainly derived from in vitro and preclinical animal models, human applications based on the regulation of mitochondrial quality in patients have not yet been approved. In this review, we summarize the key selective mitochondrial autophagy pathways and their role in prevalent chronic human diseases and highlight the potential use of specific interventions.
The Image Classification Method with CNN-XGBoost Model Based on Adaptive Particle Swarm Optimization
Authors: Jiao Wenjiang , Hao Xingwei , Qin Chao
Published: April 2021

CNN is particularly effective in extracting spatial features. However, the single-layer classifier constructed by activation function in CNN is easily interfered by image noise, resulting in reduced classification accuracy. To solve the problem, the advanced ensemble model XGBoost is used to overcome the deficiency of a single classifier to classify image features. To further distinguish the extracted image features, a CNN-XGBoost image classification model optimized by APSO is proposed, where APSO optimizes the hyper-parameters on the overall architecture to promote the fusion of the two-stage model. The model is mainly composed of two parts: feature extractor CNN, which is used to automatically extract spatial features from images; feature classifier XGBoost is applied to classify features extracted after convolution. In the process of parameter optimization, to overcome the shortcoming that traditional PSO algorithm easily falls into a local optimal, the improved APSO guide the particles to search for optimization in space by two different strategies, which improves the diversity of particle population and prevents the algorithm from becoming trapped in local optima. The results on the image set show that the proposed model gets better results in image classification. Moreover, the APSO-XGBoost model performs well on the credit data, which indicates that the model has a good ability of credit scoring.