bluuebunny/crossref_metadata_2025 huggingface dataset:
Detection and Characterization of Lasiodiplodia pseudotheobromae Associated with Stem Wilt on Ficus hirta (Vahl) and Its Fungicidal Sensitivity
Authors: He Chunping , Wu He , Hu Yue , Li Rui , Lin Jinjing , Lu Ying , Gu Ziwei , Tan Shibei , Liang Yanqiong
Published: October 2024

Ficus hirta Vahl is an important medicinal and edible plant in southern China. Typical green wilting on leaves and brown necrotic spots on the stems were observed since mid-June 2022 in an F. hirta plantation in Danzhou, Hainan (China). The disease rapidly developed, causing stem withering and plant death. The disease incidence varied from 45 to 85% prevalence, and the average disease index was 47 in the period of outbreak during June to December. Relevant hypothetical fungi were isolated from naturally infected wilt tissues, and their pathogens were preliminarily confirmed to be Lasiodiplodia pseudotheobromae through hypothetical fungal culturing, morphological characteristic observations, and pathogenicity testing on F. hirta plants. The phylogenetic tree constructed based on partial ITS, translation elongation factor (TEF1-α), and the β-tubulin gene (TUB2) further confirmed the identity of the pathogen as L. pseudotheobromae. Further research on the biological characteristics of L. pseudotheobromae showed that the optimal temperature for the growth of L. pseudotheobromae was PDA medium, with a temperature of 30 °C and pH of 6. Peptone and fructose were the optimal nitrogen and carbon sources for it. In vitro efficacy testing showed that among eleven fungicides, fluazinam and prochloraz had the highest mycelial growth inhibition, with an EC50 of 0.0477 µg/mL and 0.0996 µg/mL, respectively. And the two fungicides showed significant control on the stem wilt of F. hirta in a pot. To our knowledge, this is the first comprehensive report on the pathogen identification and biological characteristics of L. pseudotheobromae infecting the stem wilt of F. hirta in China. Our results provide important information for developing effective management measures and controlling this disease.
Two Western Pacific Tabernaemontana Species Contrast in Biomass Allocation and Leaf Physiological Plasticity to Sun and Shade
Authors: Marler Thomas E.
Published: October 2024

The ability of plants to modify biomass allocation and leaf phenotypes to best utilize available resources has been heavily studied. No Tabernaemontana species have been included in this research agenda. Therefore, Tabernaemontana pandacaqui Poir. and Tabernaemontana rotensis (Kaneh.) B.C. Stone plants were subjected to 24% or 100% sunlight and various traits were determined to compare the relative level of plasticity. Midday net carbon dioxide assimilation was greater for T. rotensis in sun-grown plants, but greater for T. pandacaqui in shade-grown plants. Saturating light intensity and midday Fv/Fm were greater for T. rotensis in sun-grown plants, but did not differ between the species for shade-grown plants. Light compensation intensity was greater for T. rotensis than T. pandacaqui in both light treatments. Apparent quantum yield was greater for T. pandacaqui shade-grown plants but was similar for the two species in sun-grown plants. Greater relative root growth in full sun compared with shade was exhibited by T. rotensis but not by T. pandacaqui. These findings indicated that T. pandacaqui develops functional traits that improve performance in shade-grown plants, and T. rotensis develops facultative traits that enable performance in sun-grown plants. These insights into how T. pandacaqui and T. rotensis respond to sun and shade conditions add to the knowledge needed to inform the selection of niche conditions when using them in managed mixed forest plantings such as conservation and restoration sites.
Life Cycle Fluoropolymer Management in Proton Exchange Membrane Electrolysis
Authors: Sinha Parikhit , Cypher Sabrine M.
Published: October 2024

Concerns over the life cycle impacts of fluoropolymers have led to their inclusion in broad product restriction proposals for per- and poly-fluorinated alkyl substances (PFAS), despite their non-bioavailable properties and low exposure potential in complex, durable goods such as non-consumer electrical products. Based on the hypothesis that manufacturers are most able to manage the environmental impacts of their products, practical engineering approaches to implementing life cycle fluoropolymer stewardship are evaluated to bridge the ongoing debate between precautionary and risk-based approaches to PFAS management. A life cycle thinking approach is followed that considers product design and alternatives, as well as the product life cycle stages of material sourcing, manufacturing, field deployment, and end-of-life. Over the product life cycle, the material sourcing and end-of-life stages are most impactful in minimizing potential life cycle PFAS emissions. Sourcing fluoropolymers from suppliers with fluorosurfactant emissions control and replacement minimizes the potential emissions of bio-available PFAS substances. A stack-as-service approach to electrolyzer operations ensures a takeback mechanism for the recycling of end-of-life fluoropolymer materials. Retaining electrolytic hydrogen’s license to operate results in over USD 2 of environmental and health benefits per kilogram of hydrogen produced from reduced greenhouse gas and air pollutant emissions compared to conventional hydrogen production via steam methane reforming.
The Role of Trace Metals in the Development and Progression of Prostate Cancer
Authors: Tshoni Unathi Albertinah , Mbonane Thokozani P. , Rathebe Phoka C.
Published: October 2024

Over the years, prostate cancer (PCa) research has been of great interest, and trace metals have attracted a lot of attention due to their association with prostate cancer development and progression. PCa has a complex etiology, with genetic, environmental, and lifestyle factors being implicated. Trace metals such as zinc (Zn), mercury (Hg), selenium (Se), lead (Pb), cadmium (Cd), manganese (Mn), arsenic (As), and nickel (Ni) have garnered much attention in recent years, suspected of having direct links to the modulation of cancer risk and progression through their impacts on prostate cancer omics (genomics, epigenetics, proteomics, and transcriptomics). This has led to them being the subject of extensive research in this regard. In this review, we explored the influence of trace metals and offered a comprehensive analysis of the current knowledge on how trace metals affect the biology of prostate cancer at a molecular level by integrating findings from the recent literature to help suggest possible directions for future research.
Bones or Stones: How Can We Apply Geophysical Techniques in Bone Research?
Authors: Szekanecz Zoltán , Besnyi Anikó , Kónya Péter , Füri Judit , Király Edit , Bertalan Éva , Falus György , Udvardi Beatrix , Kovács-Kis Viktória , Andrássy László , Maros Gyula , Fancsik Tamás , Pethő Zsófia , Gomez Izabella , Horváth Ágnes , Gulyás Katalin , Juhász Balázs , Hodosi Katalin , Sándor Zsuzsanna , Bhattoa Harjit P. , Kovács István J.
Published: October 2024

Some studies have used physical techniques for the assessment of bone structure and composition. However, very few studies applied multiple techniques, such as those described below, at the same time. The aim of our study was to determine the chemical and mineralogical/organic composition of bovine tibial bone samples using geophysical/geochemical reference techniques. X-ray diffraction (XRD), thermogravimetry (TG), Fourier-transform infrared spectrometry with attenuated total reflectance accessory (FTIR-ATR), inductively coupled mass spectrometry (ICP-MS) and inductively coupled optical emission spectrometry (ICP-OES) were applied to measure the organic and inorganic composition of 14 bovine bone samples. In addition, peripheral quantitative CT (QCT) was used to assess BMD in these bones. We were able to define the total composition of the studied bone samples. ICP-OES and ICP-MS techniques were used to determine the major and trace element composition. The X-ray analysis could detect inorganic crystalline compounds of bones, such as bioapatite, and its degree of ordering, indicating whether the bones belong to a younger or older individual. The total volatile content of the samples was calculated using TG and resulted in about 35 weight% (wt%). This, together with the 65 wt% total resulting from the chemical analysis (i.e., inorganic components), yielded a total approaching 100 wt%. As a large portion of the volatile content (H2O, CO2, etc.) was liberated from the organic components and, subordinately, from bioapatite, it could be concluded that the volatile-to-solid ratio of the examined bone samples was ~35:65. The FTIR-ATR analysis revealed that the organic portion consists of collagens containing amide groups, as their typical bands (OH, CH, CO, NC) were clearly identified in the infrared spectra. Numerous parameters of bone composition correlated with BMD as determined by QCT. In conclusion, we performed a complex evaluation of bovine bones to test multiple geophysical/geochemical techniques in bone research in association with QCT bone densitometry. From a medical point of view, the composition of the studied bones could be reliably examined by these methods.
Dietary Bacteriophage Administration Alleviates Enterotoxigenic Escherichia coli-Induced Diarrhea and Intestinal Impairment through Regulating Intestinal Inflammation and Gut Microbiota in a Newly Weaned Mouse Model
Authors: Dong Chao , Chen Yan , Ding Minfeng , Liu Yi , Chen Xingping , He Yuyong , Zou Tiande , Chen Jun , You Jinming
Published: October 2024

This study aimed to investigate the effects of dietary bacteriophage administration on diarrhea and intestinal impairment induced by enterotoxigenic Escherichia coli (ETEC) in a newly weaned mouse model. Forty-four newly weaned C57BL/6 mice were divided into four treatment groups, where they were provided either the control diet or the bacteriophage-supplemented diet, with or without ETEC infection. The results show that the bacteriophage administration resulted in increased body weight, decreased diarrhea score, and improved jejunal histopathology in ETEC-infected mice. The bacteriophage administration enhanced the intestinal barrier function of the ETEC-infected mice, as indicated by the reduced serum DAO level and the increased expression of Claudin-1, Occludin, and ZO-1 at both the mRNA and protein levels in the jejunum. Also, the bacteriophage administration resulted in a decrease in serum TNF-α and IL-1β levels, a down-regulation of TNF-α and IL-6 mRNA levels in the jejunum, and the inhibition of jejunal TLR-4/NF-κB pathway activation induced by ETEC infection. Moreover, the bacteriophage administration increased the levels of acetic acid, propionic acid, butyric acid, and total short-chain fatty acids in the caecum content. The bacteriophage administration increased the Shannon index, increased the abundance of Bacteroidota and Muribaculaceae, and decreased the abundance of Verrucomicrobiota and Akkermansiaceae in the colon contents of the ETEC-infected mice. Spearman’s correlation analysis indicates that the protective effects of bacteriophage on ETEC-induced intestinal impairment, inflammation, and intestinal barrier function are associated with regulating the abundance of Bacteroidota and Muribaculaceae in the colon contents of mice. Collectively, bacteriophage administration alleviates ETEC-induced diarrhea and intestinal impairment through regulating intestinal inflammation and gut microbiota in newly weaned mice.
Tumoricidal Activity and Side Effects of Radiolabeled Anti-NCAM [131I]-Iodine-ERIC1 in Neuroblastoma-Bearing Mice
Authors: Fischer Thomas , Dietlein Felix , Bongartz Detlev , Klehr Martin , Zimmermanns Beate , Schmidt Matthias , Mohr Angela , Mohr Fabian , Sudbrock Ferdinand , Krapf Philipp , Drzezga Alexander , Dietlein Markus , Schomäcker Klaus
Published: October 2024

Preliminary studies on a radioactive antibody against the neural cell adhesion molecule (NCAM) demonstrated a significant accumulation of [131I]I-ERIC1 in neuroblastoma tumor cells in mice. This study aims to validate the therapeutic efficacy and potential adverse effects of these radioactive immunoconjugates (RICs) in neuroblastoma-bearing mice. To determine the highest tolerated dose, healthy SCID mice received 1 to 22 MBq of [131I]I-ERIC1, with the survival time measured. Tumor response was evaluated by administering 0.8 to 22 MBq of [131I]I-ERIC1 to neuroblastoma-bearing mice and assessing tumor size and systemic toxicity through body weight, blood counts, and survival. It was observed that doses up to approximately 3 MBq per animal (150 MBq/kg) were well tolerated, whereas higher doses resulted in systemic toxicity and death. The neuroblastomas exhibited a dose-dependent response, with optimal therapeutic efficacy achieved at 1.8–2.5 MBq per animal (90–125 MBq/kg), significantly extending survival by a factor of five. The antibody ERIC1 is a promising vehicle for the transport of beta emitters into NCAM-positive tumor tissue. An optimal dosage of the [131I]I-ERIC1 antibody can be established with a balance of tumor-static effects and adverse effects, resulting in a marked extension of survival time.
Modulatory Effects of Chalcone Thio-Derivatives on NF-κB and STAT3 Signaling Pathways in Hepatocellular Carcinoma Cells: A Study on Selected Active Compounds
Authors: Papierska Katarzyna , Judasz Eliza , Tonińska Wiktoria , Kubicki Maciej , Krajka-Kuźniak Violetta
Published: October 2024

Our previous studies demonstrated the modulatory effects of new synthetic thio-chalcone derivatives in dishes on the Nrf2, NF-κB, and STAT3 signaling pathways in colon cancer cells. This study aimed to evaluate the effect of four selected active chalcone thio-derivatives on the NF-κB and STAT3 signaling pathways involved in inflammatory processes and cell proliferation in human liver cancer cells. Cell survival was assessed for cancer (HepG2) and normal (THLE-2) cell lines. Activation of NF-κB and STAT3 signaling pathways and the expression of proteins controlled by these pathways were estimated by Western blot, and qRT-PCR assessed the expression of NF-κB and STAT3 target genes. We also evaluated the impact on the selected kinases responsible for the phosphorylation of the studied transcription factors by MagneticBead-Based Multiplex Immunoassay. Among the thio-derivatives tested, especially derivatives 1 and 5, there was an impact on cell viability, cell cycle, apoptosis, and activation of NF-κB and STAT3 pathways in hepatocellular carcinoma (HCC), which confirms the possibilities of using them in combinatorial molecular targeted therapy of HCC. The tested synthetic thio-chalcones exhibit anticancer activity by initiating proapoptotic processes in HCC while showing low toxicity to non-cancerous cells. These findings confirm the possibility of using chalcone thio-derivatives in molecularly targeted combination therapy for HCC.
Genome-Wide Identification and Characterization of Diterpenoid Pathway CYPs in Andrographis paniculata and Analysis of Their Expression Patterns under Low Temperature Stress
Authors: Sun Mingyang , Li Jingyu , Xu Shiqiang , Gu Yan , Wang Jihua
Published: October 2024

Andrographis paniculata is known for its diterpenoid medicinal compounds with antibacterial and anti-inflammatory properties. However, it faces production and cultivation challenges due to low temperatures (LTs). Cytochrome P450 monooxygenases (CYPs) are key enzymes in diterpenoid accumulation. Nevertheless, the functions and LT-related expression patterns of diterpenoid pathway CYPs in Andrographis paniculata remain poorly understood. In this study, 346 CYPs were discovered in Andrographis paniculata. Among them, 328 CYPs belonged to 42 known subfamilies. The remaining 17 CYPs might have represented novel subfamilies unique to this species. A total of 65 candidate CYPs associated with diterpenoid modification were identified. Of these, 50 were transmembrane proteins, and 57 were localized to chloroplasts. The CYP71 subfamily was the most abundant and had the highest motif diversity. Promoters of all candidate CYPs commonly contained elements responsive to gibberellins (GAs), methyl jasmonate (MeJA), and abiotic stresses. Notably, the XP_051152769 protein, corresponding to a CYP gene over 40,000 bp in length, featured an extraordinarily long intron (40,751 nts). Functional elements within this intron were related to LT, GAs, and dehydration pathways. Based on the promoter element arrangement and subfamily classification, 10 representative candidate CYPs were selected. Under LT stress, significant expression changes were observed in three representative CYPs: CYP71D, ent-kaurenoic acid oxidase (KAO), and ent-kaurene oxidase (KO). KAO and KO were significantly upregulated during early LT stress. KAO and KO interacted with each other and jointly interacted with GA20OX2-like. CYP71D acted as a negative response factor to LT stress. Among the 37 proteins interacting with CYP71D, 95% were CYPs. This study provides a critical preliminary foundation for investigating the functions of diterpenoid pathway CYPs in Andrographis paniculata, thereby facilitating the development of LT-tolerant cultivars.
Effect of Endurance Exercise Training on Gut Microbiota and ER Stress
Authors: Yoon Eun Ji , Lee So Rok , Ortutu Beulah Favour , Kim Jong-Oh , Jaiswal Varun , Baek Sooyeon , Yoon Su-In , Lee Sang Ki , Yoon Jin Hwan , Lee Hae-Jeung , Cho Jin Ah
Published: October 2024

Regular exercise as part of one’s lifestyle is well-recognized for its beneficial effect on several diseases such as cardiovascular disease and obesity; however, many questions remain unanswered regarding the effects of exercise on the gut environment. This study aimed to investigate the impact of long-term endurance exercise on modulating inflammation and endoplasmic reticulum (ER) stress. Fifteen-week-old male Sprague-Dawley (SD) rats were subjected to six months of endurance treadmill training, while age-matched controls remained sedentary. Results showed that IL-6 mRNA levels in colon tissues were significantly higher in the exercise group compared to the sedentary group. Exercise activated a significant ER stress-induced survival pathway by increasing BiP and phosphorylation of eIF2α (p-eIF2α) expressions in the liver and colon, while decreasing CHOP in the liver. Gene expressions of MUC2, Occludin, and Claudin-2 were increased in the colon of the exercise group, indicating enhanced intestinal integrity. Furthermore, the data showed a positive correlation between microbiota α-diversity and BiP (r = 0.464~0.677, p < 0.05). Populations of Desulfovibrio C21 c20 were significantly greater in the exercise group than the sedentary group. Additionally, predicted functions of the gut microbial community in terms of enzymes and pathways supported the enhancement of fatty-acid-related processes by exercise. These findings suggest that prolonged endurance exercise can affect the colon environment, which is likely related to changes in inflammation, ER stress, mucin layers and tight junctions, associated with modifications in the gut microbiome.
Network Pharmacology Revealing the Therapeutic Potential of Bioactive Components of Triphala and Their Molecular Mechanisms against Obesity
Authors: Inpan Ratchanon , Sakuludomkan Chotiwit , Na Takuathung Mingkwan , Koonrungsesomboon Nut
Published: October 2024

Obesity, characterized by the excessive accumulation of fat, is a prevalent metabolic disorder that poses a significant global health concern. Triphala, an herbal combination consisting of Phyllanthus emblica Linn, Terminalia chebula Retz, and Terminalia bellerica (Gaertn) Roxb, has emerged as a potential solution for addressing concerns related to obesity. This study aimed to investigate the network pharmacology and molecular docking of Triphala to identify its bioactive ingredients and their interactions with pathways associated with obesity. The bioactive compounds present in Triphala and genes linked to obesity were identified, followed by an analysis of the protein-protein interaction networks. Enrichment analysis, including Gene Ontology analysis and Kyoto Encyclopedia of Genes and Genomes pathway analysis, was conducted. Prominent genes and compounds were selected for further investigation through molecular docking studies. The study revealed a close correlation between obesity and the AKT1 and PPARG genes. The observed binding energy between beta-sitosterol, 7-dehydrosigmasterol, peraksine, α-amyrin, luteolin, quercetin, kaempferol, ellagic acid, and phyllanthin with AKT1 and PPARG indicated a favorable binding affinity. In conclusion, nine compounds showed promise in regulating these genes for obesity prevention and management. Further research is required to validate their specific effects.
Propylthiouracil Induced Rat Model Reflects Heterogeneity Observed in Clinically Non-Obese Subjects with Nonalcoholic Fatty Liver Disease
Authors: Jin Yu , Liu Qiuyan , Wang Yuqin , Wang Bing , An Jing , Chen Qimeng , Wang Tao , Shang Jing
Published: October 2024

The prevalence of nonalcoholic fatty liver disease (NAFLD) is increasing, affecting up to 30% of the population, with approximately 20% of cases occurring in non-obese individuals. The recent shift to the term metabolic dysfunction-associated steatosis liver disease (MASLD) highlights the disease’s heterogeneity. However, there are no well-established animal models replicating non-obese NAFLD (NO-NAFLD). This study aimed to evaluate the relevance of the high-fat diet (HFD) combined with the propylthiouracil (PTU)-induced rat model in mimicking the histopathology and pathophysiology of NO-NAFLD. We first analyzed metabolic and clinical parameters between NO-NAFLD patients (Average BMI = 21.96 kg/m2) and obese NAFLD patients (Average BMI = 29.7 kg/m2). NO-NAFLD patients exhibited significantly higher levels of carnitines, phospholipids, and triglycerides. In the animal model, we examined serum lipid profiles, liver inflammation, histology, and transcriptomics. Hepatic steatosis in the HFD+PTU model at week 4 was comparable to that of the HFD model at week 8. The HFD+PTU model showed higher levels of carnitines, phospholipids, and triglycerides, supporting its relevance for NO-NAFLD. Additionally, the downregulation of lipid synthesis-related genes indicated differences in lipid accumulation between the two models. Overall, the HFD+PTU-induced rat model is a promising tool for studying the molecular mechanisms of NO-NAFLD.
Local Strain Effects on Lattice Defect Dynamics and Interstitial Dislocation Loop Formation in Irradiated Tungsten–Molybdenum Alloys: A Molecular Dynamics Study
Authors: Alnairi Marzoqa M. , Banisalman Mosab Jaser
Published: October 2024

In this study, molecular dynamics (MD) simulations were used to investigate how alloying tungsten (W) with molybdenum (Mo) and local strain affect the primary defect formation and interstitial dislocation loops (IDLs) in W–Mo alloys. While the number of Frenkel pairs (FPs) in the W–Mo alloy is similar to pure W, it is half that of pure Mo. The W–20% Mo alloy, chosen for further analysis, showed minimal FP variance after collision cascades induced by primary knock-on atoms (PKAs) at 10 to 80 keV. The research examined hydrostatic strains from −1.4% to 1.6%, finding that higher strains correlated with increased FP counts and cluster formation, including IDLs. The following two types of IDLs were identified: majority ½ <111> loops as well as <100> IDLs that formed within the initial picoseconds of the simulations under higher tensile strain (1.6%) and larger PKA energies (80 keV). The strain effects also correlated with changes in threshold displacement energy (TDE), with higher FP formation under tensile strain. This study highlights the impact of strain and alloying on radiation damage, particularly in low-temperature, high-energy environments.
Recent Advances in Aluminum Nitride (AlN) Growth by Magnetron Sputtering Techniques and Its Applications
Authors: Jadoon Nabeel Ahmad Khan , Puvanenthiram Vaigunthan , Mosa Mayada Ahmed Hassan , Sharma Ashutosh , Wang Kaiying
Published: October 2024

This review explores the processes involved in enhancing AlN film quality through various magnetron sputtering techniques, crucial for optimizing performance and expanding their application scope. It presents recent advancements in growing AlN thin films via magnetron sputtering, elucidating the mechanisms of AlN growth and navigating the complexities of thin-film fabrication. Emphasis is placed on different sputtering methods such as DC, RF, pulsed DC, and high-power impulse DC, highlighting how tailored sputtering conditions enhance film characteristics in each method. Additionally, the review discusses recent research findings showcasing the dynamic potential of these techniques. The practical applications of AlN thin films, including wave resonators, energy harvesting devices, and thermal management solutions, are outlined, demonstrating their relevance in addressing real-world engineering challenges.
Effects of Perforated Plates on Shock Structure Alteration for NACA0012 Cascade Configurations
Authors: Gall Mihnea , Dumitrescu Oana , Drăgan Valeriu , Crunțeanu Daniel-Eugeniu
Published: October 2024

To alleviate the shock boundary layer interaction adverse effects, various active or passive flow control strategies have been investigated in the literature. This research sheds light on the behavior of perforated plates as passive flow control techniques applied to NACA0012 airfoils in cascade configurations. Two identical perforated plates with shallow cavities underneath are accommodated on the upper and lower surfaces of each airfoil in the cascade arrangement. Six different cascade arrangements, including a baseline configuration with no control applied, are additively manufactured, with different perforated plate orifice sizes in the range of 0.5–1.2 mm. A high-speed wind tunnel with Schlieren optical diagnosis and wall static pressure taps is used to investigate the changes in the shock waves pattern triggered by the perforated plates. Steady 3D density-based numerical simulations in Ansys FLUENT are conducted for further analysis and validation. In the cascade configuration, the perforated plates alter the shock structure, and the strong normal shock wave is replaced by a weaker X-type shock structure. Eventually, a 1% penalty in overall total pressure loss is induced by the perforated plates because of the negative loss balance between the reduced shock losses and the enhanced viscous losses. Further studies on perforated plate geometrical features are needed to improve this outcome in a cascade arrangement.
Three-Dimensional Printing Limitations of Polymers Reinforced with Continuous Stainless Steel Fibres and Curvature Stiffness
Authors: Clarke Alison J. , Dickson Andrew N. , Milosavljević Vladimir , Dowling Denis P.
Published: October 2024

This study investigates the printability limitations of 3D-printed continuous 316L stainless steel fibre-reinforced polymer composites obtained using the Materials Extrusion (MEX) technique. The objective was to better understand the geometric printing limitations of composites fabricated using continuous steel fibres, based on a combination of bending stiffness testing and piezoresistive property studies. The 0.5 mm composite filaments used in this study were obtained by co-extruding polylactic acid (PLA), with a 316 L stainless steel fibre (SSF) bundle. The composite printability limitations were evaluated by the printing of a series of ’teardrop’ shaped geometries with angles in the range from 5° to 90° and radii between 2 and 20 mm. The morphology and dimensional measurements of the resulting PLA-SSF prints were evaluated using μCT scanning, optical microscopy, and calliper measurements. Sample sets were compared and statistically examined to evaluate the repeatability, turning ability, and geometrical print limitations, along with dimensional fluctuations between designed and as-printed structures. Comparisons of the curvature bending stiffness were made with the PLA-only polymer and with 3D-printed nylon-reinforced short and long carbon fibre composites. It was demonstrated that the stainless steel composites exhibited an increase in bending stiffness at smaller radii. The change in piezoresistance response of the PLA-SSF with load applied during the curvature bending stiffness testing demonstrated that the 3D-printed composites may have the potential for use as structural health monitoring sensors.
Early Specialization and Progress of Finalist Swimmers in World Championships and Olympic Games
Authors: Yustres Amores Inmaculada , Santos del Cerro Jesús , Rodrigo Carranza Víctor , Hermosilla-Perona Francisco
Published: October 2024

Background: The main objectives of this study were to analyze the effect of early specialization in swimming and to observe the general patterns of success of two different sport specialization models [Spanish (SPA) and United States of American (USA) swimmers] participating in World Championships (WCs) and Olympic Games (OGs) between the years 2006 and 2021 of all swimming strokes and distances. Methods: Descriptive analyses and contingency tables were examined for all the variables. Explanatory models of the z scores were estimated from age depending on the events’ distances and strokes. Quadratic regression models were developed to capture the behavior of the variable time with parabolic functions, and the significance of the global model and the predictor variables (age) were also evaluated. In addition, the optimal age (peak performance) as well as the curvature of the model were analyzed. These models were compared between SPA and USA swimmers. Results: The results showed that the main differences in the patterns to success between SPA and USA were the earlier participation of USA swimmers in high-level competitions, as well as the greater number of participants for all the strokes, events, genders, and competitions. Age peak performance in short distances was lower for Spanish swimmers, obtaining the opposite situation for long distances. Conclusions: Being a finalist in junior WCs did not influence success in the finals of the senior WCs and OGs. Main differences in general patterns of success between SPA and USA showed younger swimmers participating in short-distance events, backstroke, and butterfly for USA swimmers and older swimmers taking part in butterfly and short-distance events for SPA.
Insider Trading before Earnings News: The Role of Executive Pay Disparity
Authors: Nguyen Ann-Ngoc , Le Viet , Gregoriou Andros , Kernohan David
Published: October 2024

We investigate how executive pay disparity affects insider profits around earnings news. Our findings reveal that high pay disparity is linked to higher abnormal returns from insider purchases before positive news, suggesting insiders exploit good news for greater gains. Conversely, it is associated with lower abnormal returns from insider sales before negative news, indicating less benefit from such sales. These insights highlight the influence of pay disparity on insider trading and underscore the importance of understanding this dynamic to improve decision-making and reduce misuse of insider information.
The Influence of Fixed and Flexible Funding Mechanisms on Reward-Based Crowdfunding Success
Authors: Mamaro Lenny Phulong , Sibindi Athenia Bongani
Published: October 2024

This study examined whether fixed or flexible funding mechanisms influence crowdfunding success. Under the fixed funding mechanism, the pledges contributed to the crowdfunding campaign projects are returned to the backers if the project fails, whereas, under the flexible funding mechanism, the project creator can keep all the raised pledges, irrespective of whether the project succeeds or fails. Secondary data consisted of reward-based crowdfunding projects retrieved from The Crowd Data Centre. Logistic regression was employed to respond to research objectives. The results reveal that the fixed funding mechanism increases the probability of success more than flexible funding. Entrepreneur experience, spelling errors, and project description negatively affect crowdfunding success, and backers positively affect crowdfunding success. The findings guide entrepreneurs seeking financing to design and choose an appropriate funding mechanism that effectively reduces the failure rate. Although many entrepreneurs seek funding in the crowdfunding market, relatively little research has been conducted on the influence of flexible or fixed funding mechanisms on crowdfunding success in Africa. This study provides entrepreneurs with appropriate financing strategies that enhance crowdfunding success. The empirical literature indicates that the flexible funding mechanism creates distrust among backers due to unrealistic target amounts.
Impact of Acoustic and Optical Phonons on the Anisotropic Heat Conduction in Novel C-Based Superlattices
Authors: Talwar Devki N. , Becla Piotr
Published: October 2024

C-based XC binary materials and their (XC)m/(YC)n (X, Y ≡ Si, Ge and Sn) superlattices (SLs) have recently gained considerable interest as valuable alternatives to Si for designing and/or exploiting nanostructured electronic devices (NEDs) in the growing high-power application needs. In commercial NEDs, heat dissipation and thermal management have been and still are crucial issues. The concept of phonon engineering is important for manipulating thermal transport in low-dimensional heterostructures to study their lattice dynamical features. By adopting a realistic rigid-ion-model, we reported results of phonon dispersions ωjSLk→ of novel short−period XCm/(YC)n001 SLs, for m, n = 2, 3, 4 by varying phonon wavevectors k→SL along the growth k|| ([001]), and in-plane k⊥ ([100], [010]) directions. The SL phonon dispersions displayed flattening of modes, especially at high-symmetry critical points Γ, Z and M. Miniband formation and anti-crossings in ωjSLk→ lead to the reduction in phonon conductivity κz along the growth direction by an order of magnitude relative to the bulk materials. Due to zone-folding effects, the in-plane phonons in SLs exhibited a strong mixture of XC-like and YC-like low-energy ωTA, ωLA modes with the emergence of stop bands at certain k→SL. For thermal transport applications, the results demonstrate modifications in thermal conductivities via changes in group velocities, specific heat, and density of states.
Production and Characterization of Fine-Grained Multielement AlCoxCrFeNi (x = 1, 0.75, 0.5) Alloys for High-Temperature Applications
Authors: Shaik Khaja Naib Rasool , Bortolotti Mauro , Leizaola Iñaki , Lagos Gomez Miguel Angel , Menapace Cinzia
Published: October 2024

In the present work, three different AlCoxCrFeNi (x = 1, 0.75, 0.5) alloys were produced through the mechanical milling of powders and spark plasma sintering. These alloys were characterized in terms of their microstructural, mechanical, and oxidation behaviors. Mechanical milling and spark plasma sintering were chosen to achieve a fine and homogeneous microstructure. Pore-free samples were produced by properly setting the sintering parameters. The unavoidable uptake of oxygen from the powders when exposed to air after milling was advantageously used as a source of oxides, which acted as reinforcing particles in the alloy. Oxidation behavior, studied through TGA tests, showed that decreasing the Co content promotes better oxidation protection due to the formation of a dense, compact Al2O3 layer. The alloy containing the lowest amount of Co is considered a good candidate for high-temperature structural applications.
The Effect of Sodium Hexametaphosphate on the Dispersion and Polishing Performance of Lanthanum–Cerium-Based Slurry
Authors: Mei Yan , Chen Wenjuan , Chen Xuean
Published: October 2024

A lanthanum–cerium-based abrasive composed of CeO2, LaOF, and LaF3 was commercially obtained. The effect of sodium hexametaphosphate (SHMP) on powder dispersion behavior was systematically investigated using the combined techniques of liquid contact angle, turbidity, zeta potential (ZP), scanning electron microscopy (SEM), powder X-ray diffraction (XRD) combined with Rietveld refinements, X-ray photoelectron spectroscopy (XPS), and polishing tests. The results indicated that the addition of 0.5 wt.% SHMP dispersant to the 5 wt.% lanthanum–cerium-based slurry produced the most stable suspension with a high turbidity of 2715 NTU and a low wetting angle of 45°. The as-obtained slurry displayed good surface polishing quality for K9 glass, with low surface roughness (Ra) of 0.642 and 0.515 nm (in the range of 979 × 979 μm2) at pH = 6 and 11, respectively, which corresponds to the fact that it has local maximum absolute values of ZP at these two pH values. SEM images demonstrated that after appropriate grafting of SHMP, the particle aggregation was reduced and the slurry’s dispersion stability was improved. In addition, the dispersion mechanism was explained based on the principle of complexation reaction, which reveals that the dispersant SHMP can increase the interparticle steric hindrance and electrostatic repulsions. In an acidic environment, steric hindrance dominates, while electrostatic repulsion prevails under alkaline conditions. As expected, this polishing slurry may find potential applications in manufacturing optical devices and integrated circuits.
A Review of the Degradation Research on the Single-Lap Bolted Joint
Authors: Wang Sheng’ao , Zhu Min , Guo Ming , Wu Fei
Published: October 2024

Bolted joints, with their advantages of simple structure and convenient disassembly and assembly, are widely used in complex equipment fields such as aerospace systems and weaponry. Subject to complex mechanical loads, the contact surfaces may undergo nonlinear behaviors such as contact–separation and viscous slip, leading to the nonlinear degradation of the connection stiffness, which severely threatens the safety and reliability. These have driven research on bolted joints to span multiple disciplines, from interfacial micro-friction to macro-structural dynamics. Therefore, from the field of micro-friction to macro-dynamics, this review summarizes and analyzes three major degradation models, outlines the experimental development in connected structures, and provides an overview of the numerical analysis methods for degradation simulation. This paper also looks forward to the development directions for future research on the degradation of connected structures.
How Encephalopathy Impacts Language Ability: A Scoping Review of the Linguistic Abilities of Adults with Developmental and Epileptic Encephalopathy
Authors: Papatheodorou Ioanna , Stavrakaki Stavroula , Koukoulioti Vasiliki , Spilioti Martha , Kimiskidis Vasileios
Published: October 2024

Background and Objectives: Developmental and epileptic encephalopathy refers to a group of conditions where patients experience abnormal development due to various causes as well as frequent epileptiform discharges that ultimately contribute, in an independent and additive fashion, to cognitive and linguistic impairments. The language and cognition outcome of these patients in adulthood has been understudied. This paper aims to present a scoping review of linguistic abilities in adults with developmental and epileptic encephalopathy to determine the extent to which language outcomes in adulthood and their relation to cognitive outcomes have been studied. Design: Two online databases were searched and the methodological framework by Arksey & O’Malley (2005) was adopted. Results: Out of the 27 selected studies, only 13 exclusively examined adults, 15 were group studies, 5 were case studies and 7 were case series. A total of 9 out of the 15 group studies provided individual results for adults. Twenty-two studies included a follow-up examination. Twenty-three studies addressed the relationship between language and cognition. The selected studies indicate the presence of language impairments, which are nevertheless differentially manifested in the syndromes under investigation, whereas individual variability is also reported. Aspects of cognition seem to correlate with linguistic abilities. Conclusions: In sum, despite variability in linguistic abilities, language deficits constitute a significant aspect of the clinical profile of many adults with developmental and epileptic encephalopathy, a finding that should be taken into account for the treatment protocols of these individuals.
Prophylactic Administration of Perampanel for Post-Stroke Epilepsy (PROPELLER Study): A Trial Protocol
Authors: Yamada Shuichi , Nakagawa Ichiro , Kotsugi Masashi , Asada Kiyoshi , Kasahara Masato
Published: October 2024

Background: Post-stroke epilepsy can reduce patients’ abilities to carry out various activities of daily living. Despite their importance in preventing the onset of post-stroke epilepsy, the prophylactic administration of antiepileptic drugs is controversial due to a lack of high-level clinical research. In this study, we initiated a prospective interventional study of prophylactic antiepileptic drug administration in patients with a subcortical hemorrhage, who are at the highest risk of developing epilepsy after experiencing a stroke. Methods: The study was conducted in a single-center setting and was a single-arm study with no control group; the case entry period started in November 2023 and is due to end in March 2025. Only cases with a subcortical hemorrhage will be included. The treatment regimen used in this study is 2 mg of perampanel per day. Perampanel will be administered for one year, followed by two years of follow-up, for a total study period of three years. The primary endpoint will be the development of epilepsy. Results: Perampanel administration is expected to reduce the incidence of post-stroke epilepsy in comparison to the results of previous reports on the use of alternative treatments. Conclusions: The results of this study will provide new insights into the prevention of post-stroke epilepsy. The relatively small size of this study makes it difficult to provide strong evidence of the efficacy of perampanel, but it may serve as a basis for larger clinical trials.
Learning Gradient-Based Feed-Forward Equalizer for VCSELs
Authors: Srinivasan Muralikrishnan , Pourafzal Alireza , Giannakopoulos Stavros , Andrekson Peter , Häger Christian , Wymeersch Henk
Published: October 2024

Vertical cavity surface-emitting laser (VCSEL)-based optical interconnects (OI) are crucial for high-speed data transmission in data centers, supercomputers, and vehicles, yet their performance is challenged by harsh and fluctuating thermal conditions. This paper addresses these challenges by integrating an ordinary differential equation (ODE) solver within the VCSEL communication chain, leveraging the adjoint method to enable effective gradient-based optimization of pre-equalizer weights. We propose a machine learning (ML) approach to optimize feed-forward equalizer (FFE) weights for VCSEL transceivers, which significantly enhances signal integrity by managing inter-symbol interference (ISI) and reducing the symbol error rate (SER).
Was Paul Within Judaism, Within Israel or Within Israel’s Messiah?
Authors: Du Toit Philip La Grange
Published: October 2024

In NT scholarship, the Paul Within Judaism approach has gained considerable momentum. In this approach to Paul’s discourse on identity, a distinction is drawn between “Jewish” and gentile followers of Christ. “Jewish” followers, including Paul, are considered those that remain fully Torah-observant, whereas gentile followers are regarded as not fully Torah-observant, especially with respect to circumcision. In this contribution, Paul’s identity in relation to first-century “Judaism” and/or historical Israel is reconsidered. The main question that is asked in this regard is whether the Paul Within Judaism approach is a viable position in light of the hermeneutical difficulties surrounding first-century “Judaism”, as well as Paul’s own rhetoric around the Ἰουδαῖοι (“Jews”/“Judaeans”). Another question that is asked is whether Ἰσραήλ (“Israel”) and the Ἰουδαῖοι point to the exact same entity. Would it be more accurate to see Paul as being within Israel as one of God’s covenant people than as remaining a Ἰουδαῖος (“Jew”/“Judaean”)? Or did Paul leave his identity as a Ἰουδαῖος or as part of Israel behind for a new identity to be defined around Israel’s Messiah only? Lastly, it is considered whether Paul’s discourse on identity leaves room for an identity in Christ that is inclusive of an identity as a Ἰουδαῖος or as being part of Israel, or whether the identity in Christ excludes the latter.
Harmonic Vibration Analysis in a Simplified Model for Monitoring Transfemoral Implant Loosening
Authors: Zhou Qingsong , Rose Louis Raymond Francis , Ebeling Peter , Russ Matthias , Fitzgerald Mark , Chiu Wing Kong
Published: October 2024

A simplified axisymmetric model of a transfemoral osseointegration implant was used to investigate the influence of the contact condition at the bone–implant interface on the vibrational response. The experimental setup allowed the degree of implant tightness to be controlled using a circumferential compression device affixed to the bone. Diametrically placed sensors allowed torsional modes to be distinguished from flexural modes. The results showed that the structural resonant frequencies did not shift significantly with tightness levels. The first torsional mode of vibration was found to be particularly sensitive to interface loosening. Harmonics in the vibrational response became prominent when the amplitude of the applied torque increased beyond a critical level. The torque level at which the third harmonic begins to rise correlated with implant criticality, suggesting a potential strategy for early detection of implant loosening based on monitoring the amplitude of the third harmonic of the torsional mode.
Bioactives in Berry Fruits with Emphasis on In Vitro Bioaccessibility for Human Nutrition
Authors: Varzaru Iulia , Untea Arabela Elena , Oancea Alexandra Gabriela , Saracila Mihaela , Vlaicu Petru Alexandru
Published: October 2024

This study aimed to investigate the bioaccessibility and biostability of carotenoids, vitamin E isomers, and individual polyphenolic compounds after the in vitro gastrointestinal digestion of two types of berries (raspberry and blackberry fruits). The results of the polyphenols analysis showed that raspberry fruits contained higher concentrations of hydroxybenzoic acids, hydroxycinnamic acids, flavanols, and flavonols compared to blackberry fruits, but exhibited the lowest bioaccessibility values for all the studied polyphenol classes. Ellagic acid represented 13.63% and 2.65% of the hydroxybenzoic acids in raspberry and blackberry fruits. The hydroxybenzoic acids exhibited the highest bioaccessibility index in the intestinal phase of both types of berries, and gallic acid emerged as one of the most bioaccessible phenolic compounds. The bioaccessibility of carotenoids ranged between 15.7 and 17.30% for lutein, 5.52 and 7.56% for astaxanthin, and 7.85 and 9.93% for canthaxanthin, with elevated values being observed in raspberry fruits. Although vitamin E and carotenoids follow a similar path for absorption, the bioaccessibility of vitamin E isomers was higher than that of carotenoids, with γ-tocopherol being the most bioaccessible isomer in both raspberries and blackberries. Knowing the bioaccessibility of food constituents during digestion is crucial, as the potential effectiveness of bioactives for human health largely depends on the bioavailability of these molecules.