bluuebunny/crossref_metadata_2025 huggingface dataset:
Genetic Analysis and Prenatal Diagnosis of Acute Intermittent Porphyria Caused by Novel Classical Splicing Variant in the Insertion Region of 29-Residue Specific to Human HMBS Protein
Authors: Liang Lei , Meng Haixia , Wu Haotian , Zhao Jianrong
Published: February 2023

Background: Acute intermittent porphyria (AIP; OMIM#176000) is caused by mutations in the hydroxymethylbilane synthetase (HMBS) gene, which encodes the third enzyme of the heme biosynthesis pathway. A 29-residue insert (residues 296-324) exists at the interface between domains 1 and 3 of human HMBS (hHMBS), and its function remains unknown. In this study, on the HMBS gene of an AIP pregnant woman from China, we predicted the damage to 29-residue insert regions of a new classical splicing variant, and made prenatal diagnosis of the fetus of this pregnant woman.
Methods: We extracted peripheral blood from pregnant women and their husbands, as well as fetal amniotic fluid cells, and analyzed gene expression using prenatal whole exon sequencing and Sanger sequencing. RNA was extracted from peripheral blood of pregnant women and reverse transcribed into cDNA for alternative splicing verification. The protein structure of abnormal splicing caused by variant was predicted using I-TASSER and PyMOL software. Using AMBER14sb software, we simulated protein molecular dynamics.
Results: Pregnant women and her fetuses have a classical splicing variant in the HMBS gene, c.912+1G>C. The husband of the pregnant woman did not detect the variant. There are no reports of this variant in the literature. Results of peripheral blood transcription tests in pregnant women showed that c.912+1G>C retained the intron 13 and caused the exon 13 skipping. Homology modeling and molecular dynamics analysis revealed that the variant affected the HMBS protein's secondary structure, which led to functional differences.
Conclusion: A new classical splicing mutation with pathogenicity of HMBS gene c.912+1G>C was identified in this study, which expands the molecular heterogeneity of AIP and provides key information for genetic diagnosis.
Genome-wide Association Study Meta-analysis of Blood Pressure Traits and Hypertension in Sub-Saharan African Populations: An AWI-Gen Study
Authors: Singh Surina , Choudhury Ananyo , Hazelhurst Scott , Crowther Nigel , Boua Palwende , Sorgho Hermann , Agongo Godfred , Nonterah Engelbert , Micklesfield Lisa , Norris Shane , Kisiangani Isaac , Mohamed Shukri , Gomez-Olive Francesc , Tollman Stephen , Choma Solomon , Brandenburg Jean-Tristan , Ramsay Michele
Published: February 2023

Most hypertension-related genome-wide association studies (GWAS) focus on non-African populations, despite hypertension (a major risk factor for cardiovascular disease) being highly prevalent in Africa. The AWI-Gen study GWAS meta-analysis for blood pressure-related traits (systolic and diastolic blood pressure, pulse pressure, mean-arterial pressure and hypertension) from three sub-Saharan African geographic regions (N=10,775), identified two genome-wide significant signals (p<5E-08): systolic blood pressure near P2RY1 (rs77846204; intergenic variant, p=4.25E-08) and pulse pressure near Linc01256 (rs80141533; intergenic variant, p=4.25E-08). No genome-wide signals were detected for the AWI-Gen GWAS meta-analysis with previous African-ancestry GWASs (UK Biobank (African), Uganda Genome Resource). Suggestive signals (p<5E-06) were observed for all traits, with 29 displaying pleiotropic effects and several replicating known associations. Polygenic risk scores developed from studies on different ancestries had limited transferability, with multi-ancestry models providing better prediction. This study provides insights into the genetics and physiology of blood pressure variation in African populations.
Effects of fullerenol C60 on liver tissue in liver ischemia reperfusion injury in rats undergoing sevoflurane anesthesia Subtitle: Fullerenol C60 in liver ischemia reperfusion injury in rats
Authors: Yavuz Aydın , Tuna Ayca Tas , Ozdemir Cagrı , Mortas Tülay , Küçük Ayşegül , Kasapbaşı Esat , Arslan Mustafa , Kavutçu Mustafa , Kurtipek Omer
Published: February 2023

The aim of this study was to investigate the effects of fullerenol C60 on rat liver tissue in liver ischemia reperfusion injury (IRI) model undergoing sevoflurane anesthesia to evaluate the ability of nanoparticles to prevent hepatic complications. A total of 36 adult female Wistar Albino rats were divided into six groups each containing six as follows: sham group (Group S), the fullerenol C60 group (Group FC60), the ischemia-reperfusion group (Group IR), and the ischemia-reperfusion-sevoflurane group (Group IR-Sevo), ischemia-reperfusion-fullerenol C60 group (Group IR-FC60), ischemia-reperfusion-fullerenol C60-sevoflurane group (Group IR-FC60-Sevo). Fullerenol C60 100 mg/kg was administrated in Group IR-FC60 and IR-FC60-Sevo. In IR groups 2 hours of ischemia and 2 hours of reperfusion were performed. At the end of the reperfusion, liver tissues were removed for biochemical assays, and histopathological examinations. Hepatocyte degeneration, sinüsoidal dilatation, prenecrotic cell, MN celluler infiltration in the parenchyma were significantly higher in Group IR than all other groups. TBARS level was significantly higher in Group IR than other groups and the lowest TBARS level was in Group IR-FC60 than other groups except Group S and FC60. CAT and GST activities reduced in Groups IR than all other groups. Fullerenol C60 has protective effects against liver IR injury in rats under sevoflurane anesthesia. The use of fullerenol C60 could reduce the adverse effects of IRI and associated costs in liver transplantation surgery.
Disabled Students in Fieldwork Education: Academic Coordinators’ Perspectives on Accommodation Process
Authors: Mayer Yael , Mira Fernanda , Zaman Shahbano , Jarus Tal
Published: February 2023

Learning in a fieldwork setting is a requirement of many professional postsecondary programs, and prominent in health professions education. However, fieldwork education requirements can create additional challenges for disabled students. Academic coordinators, who are responsible for students’ placements, hold an important role in supporting disabled students in their fieldwork education. Nevertheless, studies on the roles and experiences of academic coordinators supporting students who require accommodations are limited. This exploratory study examined the perspectives of academic coordinators regarding their practices in supporting disabled students in health professions programs. The study employed a mixed methods design. Fifteen academic coordinators from occupational therapy programs completed a quantitative survey. Then, five of the academic coordinators participated in semi-structured interviews that supported the interpretation of the quantitative results. Academic coordinators faced complex barriers in providing disabled students the support they needed to succeed in fieldwork. Two main themes emerged: (1) ACs constantly navigated tensions with institutional norms regarding fieldwork and (2) ACs manage fieldwork accommodations within ever-changing human dynamics and social norms. Collaborative practices with fieldwork educators enabled the academic coordinators to overcome some of these barriers and create a more inclusive fieldwork education experience for disabled students. Further institutional and community resources are required.
Comparative Probability Based Seismic Safety Assessment of Base Isolated Building Frames
Authors: Narjabadifam Peyman , Mollaei Somayeh , Babaei Mehdi , Mousavi Navid
Published: February 2023

Seismic fragility curves are used to assess the probability of vulnerability of structures at various damage states. This research deals with the effects of different isolation systems on the seismic safety of a two-dimensional reinforced concrete moment-resisting frame. The reference structure was a hotel building in California. A comparative probability-based seismic safety of building components was investigated on the superstructure hypothetically fitted with various isolation systems. In this regard, two categories of isolation systems including, rubber-based and friction‐based were selected here. The High Damping Rubber Bearings and Friction Pendulum Systems were considered here. Incremental Dynamic Analyses were carried out for a suite of earthquake records to develop the fragility curves considering modeling, demand, and capacity uncertainties. Based on the main results, the influence of seismic isolation systems on the reduction of failure probability of building rather than fixed base (un-retrofitted) model was observed. Moreover, the High Damping Rubber Bearings were more reliable than Friction Pendulum Systems in the limit states studied. No significant discrepancy was, however, seen in the performance of building fitted with isolation systems at higher damage states.
The taxonomy of subjective cognitive decline: proposal and first clinical evidence from the Geneva memory clinic cohort
Authors: Ribaldi Federica , Palomo Rafael , Altomare Daniele , Scheffler Max , Assal Frederic , Ashton Nicholas J. , Zetterberg Henrik , Blennow Kaj , Abramowicz Marc , Garibotto Valentina , Chicherio Christian , Frisoni Giovanni B.
Published: February 2023

Background: Subjective Cognitive Decline (SCD) is characterized by subjective cognitive complaints without objective cognitive impairment and is considered a risk factor for cognitive decline and dementia. However, most SCD patients will not develop neurodegenerative disorders, yet they may suffer from minor psychiatric, neurological, or somatic comorbidities. The aim of the present study is to provide a taxonomy of the heterogeneous SCD entity by isolating homogenous SCD subgroups with specific clinical features and cognitive trajectories.
Methods: Participants were fifty-five SCD individuals consecutively recruited at the Geneva Memory Center. Based on clinical reports, they were classified into three clinically pre-defined subgroups: (i) those with psychological or psychiatric comorbidities (Psy), (ii) those with somatic comorbidities (SomCom), (iii) and those with no apparent cause (NAC). Baseline demographics, clinical, cognitive, and biomarker differences among the SCD subgroups were assessed. Longitudinal cognitive changes (average 3 years follow-up) were modeled using a linear mixed model.
Results: Out of the 55 SCD cases, 16 were SomCom, 18 Psy, and 21 NAC. 47% were female, mean age was 71 years. We observed higher frequency of APOE ε4 carriers in NAC (53%) compared to SomCom (14%) and Psy (0%, P=0.023) and lower level of plasma Aβ42 in NAC (6.8±1.0) compared to SomCom (8.4±1.1; P=0.031). SomCom subjects were older (74 years) than Psy (67 years, P=0.011), and had greater medial temporal lobe atrophy (1.0±1.0) than Psy (0.2±0.6) and NAC (0.4±0.5, P=0.005). SomCom have worse episodic memory performances (14.5±3.5) than Psy (15.8±0.4) and SomCom (15.1±0.7, P=0.032). We observed a slightly steeper, yet not statistically significant, cognitive decline in NAC (β=-0.48) compared to Psy (β=-0.28) and SomCom (β=-0.24).
Conclusions: NAC feature higher proportion of APOE ε4 carriers, lower plasma Aβ42, worse memory performance, and a trend towards steeper cognitive decline than SomCom and Psy. Taken together, these findings suggest that NAC are at higher risk of cognitive decline due to AD. The proposed clinical taxonomy might be implemented in clinical practice to identify SCD at higher risk. However, such taxonomy should be tested on an independent cohort with larger sample size.
Nuclear receptor co-repressor NCOR2 and its relation to GPER with prognostic impact in ovarian cancer
Authors: Reichenbach Juliane , Fraungruber Patricia , Mayr Doris , Buschmann Christina , Kraus Fabian B T , Topalov Nicole Elisabeth , Chelariu-Raicu Anca , Kolben Thomas , Burges Alexander , Mahner Sven , Kessler Mirjana , Jeschke Udo , Czogalla Bastian , Trillsch Fabian
Published: February 2023

Purpose
The significance of the non-classical G-protein coupled estrogen receptor (GPER) as positive or negative prognostic factor for ovarian cancer patients remains still controversial. Recent results indicate that an imbalance of both co-factors and co-repressors of nuclear receptors regulate ovarian carcinogenesis by altering the transcriptional activity through chromatin remodeling. The present study aims to investigate whether the expression of the nuclear co-repressor NCOR2 plays a role in GPER signaling which thereby could positively impact overall survival rates of ovarian cancer patients.
Methods
NCOR2 expression was evaluated by immunohistochemistry in a cohort of 156 epithelial ovarian cancer (EOC) tumor samples and correlated to GPER expression. The correlation and differences in clinical and histopathological variables as well as their effect on prognosis was analyzed by Spearman´s correlation, Kruskal-Wallis-test and Kaplan-Meier-estimates.
Results
Histologic subtypes were associated with different NCOR2 expression patterns. More specifically, serous and mucinous EOC demonstrated a higher NCOR2 expression (P = 0.008). In addition, high nuclear NCOR2 expression correlated significantly with high GPER expression (cc = 0.245, P = 0.008). A combined evaluation of both, high NCOR2 (IRS > 6) and high GPER (IRS > 8) expression revealed an association of a significantly improved overall survival (median OS 50.9 versus 105.1 months, P = 0.048).
Conclusion
Our results support the hypothesis that nuclear co-repressors such as NCOR2 may influence the transcription of target genes in EOC such as GPER. Understanding the role of nuclear co-repressors on signaling pathways will allow a better understanding of the factors involved in prognosis and clinical outcome of EOC patients.
Light in the box - photobiological examination chamber with light trap ventilation system for studying fungal surface cultures illustrated with Metarhizium brunneum and Beauveria brongniartii
Authors: Vrabl Pamela , Zottele Maria , Colleselli Lucia , Schinagl Christoph Walter , Mayerhofer Laura , Siewert Bianka , Strasser Hermann
Published: February 2023

Due to their versatile lifestyle as saprophytes, endophytes and entomopathogens, fungi of the genera Metarhizium and Beauveria are exposed to varying illumination conditions in their natural habitats, which makes a thorough adaptation to light very likely. While the few available studies for these genera support this assumption, research in this field is still in its infancy and the data material restricted to only a few fungal species. Thus, the aim of this work was to explore how light influences growth, conidial production and secondary metabolite formation of two industrial relevant strains of M. brunneum (MA 43, formerly M. anisopliae var. anisopliae BIPESCO 5/F52) and B. brogniartii (BIPESCO 2). To achieve this, we constructed an easily adjustable illumination device for highly standardized photophysiological studies of fungi on Petri dishes, the so-called LIGHT BOX. With the aid of this device, M. brunneum and B. brongniartii were grown on S4G or S2G agar at 25°C for 14 days either in complete darkness or under constant illumination with red light (λpeak = 635 nm), green light (λpeak = 519 nm) or blue light (λpeak = 452 nm). In addition, for each wavelength the effect of different illumination intensities was tested, i.e., intensities of red light ranging from 22.1 ± 0.1 to 136.5 ± 0.3 µW cm-², green light from 16.5 ± 0.1 to 96.2 ± 0.1 µW cm-², and blue light from 56.1 ± 0.2 to 188.9 ± 0.6 µW cm-². Both fungi strongly responded in terms of growth, conidial production, pigmentation and morphology to changes in the wavelength and irradiation intensity. The wavelength-dependent production of the well-known secondary metabolite oosporein which is secreted by the genus Beauveria in particular, was also increased under green and blue light exposure. The established LIGHT BOX system allows not only to optimize spore production yields with these biotechnologically relevant fungi, but also allows the photobiological exploration of other fungi.
Narratives of Polish-Ukrainian interaction in Polish documentaries
Authors: Rosinska Olena
Published: February 2023

Introduction. The research aims at studying key narratives being transmitted in television documentaries in Ukraine and Poland in the context of displaying two peoples’ interaction in historical, cultural and social fields. The television product elucidated the image of Ukraine and the Ukrainian for a Polish viewer became the material for analysis.
Methods of research: content analysis as a systematic research technique of objective description of the existing content of communication in the media material, which was used at the stage of studying the general problematic direction of the documentary series; the method of analysis, comparison, and synthesis used in determining the typology of images and problems, narrative analysis as a way of evaluating existing mental attitudes that are realized through implemented narratives.
Results and discussions. The makers of «The Ukrainians» series tell about available failures to agree and problematic areas in the Polish-and-Ukrainian discourse. The editor-inchief of TVP Dokument Tomasz Piechal says: «We wanted this series to become a possibility for everybody to learn each other better. It has been an important unfinished theme and now, when the Russian aggression caused even more significant migration from Ukraine to Poland, it has become even more important. We have to begin becoming more familiar» [12]. Thus, the advance learning of this problem is topical as never before.
Consumer Preference for Microgreens in the Presence of LED Lights and Information Treatments
Authors: Ricks Jocey , Secor William , Campbell Benjamin L.
Published: February 2023

Since microgreens entered the market in the 1980s and 1990s, their use has expanded far beyond high-end restaurants. Most microgreens are grown in greenhouses with supplemental lighting (i.e., artificial lighting in addition to sunlight). Supplemental lighting usually includes high-pressure sodium (HPS) or light-emitting diodes (LEDs). HPS is the most common type of supplemental lighting, while LEDs are becoming more common. This article examines consumer preference and willingness to pay (WTP) for microgreens grown with LED lighting compared with HPS lighting and sunlight in the presence of different amounts and types of information. We find that negative information harms WTP, and positive information has little to no impact on WTP. We also examine how other attributes (i.e., price, location produced, production type, location purchased) impact WTP.
Residual Lignocellulosic Biomass in the Production of HMF
Authors: Godoy Cristian , Valderrama Patrícia , Furtado Andreia , Boroski Marcela
Published: February 2023

5-Hydroxymethylfurfural (HMF) can be obtained from the dehydration of monosaccharides present in biomass, which helps add value to the raw material. The present work sought to study the relationship between the parameters of biomass characterization, including the content of reducing sugars, total soluble solids and the mid-infrared (MIR) and near-infrared (NIR) spectra, and the formation of HMF after synthesis. A thorough analysis was conducted in order to evaluate the residual agricultural biomass samples from sugarcane bagasse, corn stover, cassava branch, banana pseudostem and mango fruits after the drying (45 ºC maximum) and ball mill grinding pre-processing procedure. HMF synthesis was performed using 0.50 mol L-1 HCl and n-butanol as extracting solvent. The analytical quantification of HMF and furfural (co-product of synthesis) was performed using high-performance liquid chromatography coupled to diode-array detector (HPLC-DAD). The highest efficiency of the synthesis of HMF was observed for the mango fruits (55.0 kg ton-1) and sugarcane bagasse biomass (47.5 kg ton-1) samples. The application of data fusion and principal component analysis (PCA) allowed us to identify the samples with greater potential for HMF synthesis.
Application of Disposable Pipette Extraction for Determination of Yb by High‑Resolution Continuum Source Graphite Furnace Atomic Absorption Spectrometry Using Lanthanum as a Chemical Modifier
Authors: Betiolo Diogo , Cadorim Heloisa , Carasek Eduardo , Maranhão Tatiane
Published: February 2023

The use of extraction technique with disposable tips (DPX) was applied for the first time for the extraction and preconcentration of Yb in samples of liquid waste from the petrochemical industry and environmental water using bioabsorbent cork. The use of cork as an extracting phase in the determination of Yb by high-resolution continuum source graphite furnace atomic absorption spectrometry (HR-CS GF AAS) was evaluated. The pyrolysis and atomization temperatures were optimized at 1400 and 2400 ºC, respectively. H2SO4, HNO3 and HCl acids were compared in the steps of cleaning the extraction phase and desorption of the analyte by DPX. The best extraction in the selected conditions was obtained with pH 8.0 and 5% v/v HCl. The recoveries were close to 100% for residual water and drinking water, 33 to 77% for produced water and 64 to 93% for river water. Tests with interfering ions in different concentrations were performed. The relative limit of quantification (LOQ) and limit of detection (LOD) were estimated at 0.03 and 0.01 µg L-1, respectively. The total time for the preparation of the samples by DPX was close to 1 min per sample. Extraction and pre-concentration were observed using DPX with a preenrichment factor of approximately 4 times.
Flood risk assessment using analytical hierarchy process: A case study from the Cheliff-Ghrib watershed, Algeria
Authors: Mokhtari Elhadj , Mezali Farouk , Abdelkebir Brahim , Engel Bernard
Published: February 2023

Flooding is one of the most devastating natural disasters brought on by climate change in North Africa. The occurrence of flood risk is due to a combination of natural and man-made variables, necessitating a better knowledge of its spatial scope. The goal of this study is to locate and map flood-prone regions in the Cheliff-Ghrib watershed. Within the ArcGIS interface, this study is based on the integration of multi-criteria data such as slope, drainage density, type of soil, rainfall, population density, land use and sewer system density. For flood risk assessment and mapping, the Analytic Hierarchy Process (AHP) technique was employed as a multi-criteria analysis, allowing the integration of numerous factors under two criteria namely, hazards and vulnerability. The AHP flood risk map reveals that areas at high and extremely high risk of flooding cover 22.5% of the study area. According to the findings, the Cheliff-Ghrib watershed is extremely vulnerable to flooding. Eight of the Chelliff-Ghrib watershed's 15 municipalities (8/15) are at high risk of flooding, necessitating the development of efficient flood mitigation solutions for future flood events.
Experimental and DFT study of Cu(II) removed by Na-montmorillonite
Authors: Wang Danqi , Wang Ruicong , Peng Wencai , Zhang Jinli , Wang Yi , Huang Minghui , Zhang Na , Duan Yanan , Fang Ying
Published: February 2023

The experimental and theoretical studies on the adsorption of Cu(II) on the surface of Na-montmorillonite (Na-Mt) were reported. Effects of batch adsorption experimental parameters were studied. Density functional theory and molecular dynamics simulations were used to study the adsorption of Cu(II) on montmorillonite (001) surface. The adsorption reached equilibrium within 80 min and the adsorption capacity was 35.23 mg·g−1 at 25 °C. The adsorption data of Cu(II) were consistent with pseudo-second-order kinetics and Langmuir isotherm models. The adsorption process was dominated by physical adsorption (Ea was 37.08 kJ·mol−1) with spontaneous endothermic behavior. The influence of coexisting cations on the adsorption capacity of Cu(II) was Mg(II) > Co(II) > Ca(II) > Na(I). The simulation results demonstrated that there were no significant differences in the adsorption energy of Cu(II) at the four adsorption sites on the montmorillonite (001) surface. Cu(II) had more electron transfer than Na(I). The diffusion coefficient of Cu(II) in the aqueous solution system containing montmorillonite was 0.85×10−10 m2·s−1. Considerable amounts of Cu(II) ions were adsorbed at a distance of 0.26 and 2.25 Å from the montmorillonite (001) surface. The simulation results provided strong supporting evidence for experimental conclusions.
Burzenie i tworzenie schematów inicjacyjnych w filmach Felliniego
Authors: Anna Anna
Published: February 2023

The period of going through puberty for Fellini himself was turbulent and unusual. In various interviews, the director gives his own memories from that time a different narrative – he changes the “testimonies” and modifies the facts. Equally inconsistent with culturally and socially created initiation models are the fates of the protagonists in his films, who go through the period of “growing up” and “puberty” at different ages and in different ways, usually contradicting stereotypical images, however not always. The director seems to use well-known patterns and show them in a crooked mirror or transplant them onto non-obvious grounds. The protagonists are often shown on the verge of change, facing the problem of crossing a specific line and reaching “maturity” (this concept also encapsulates various values). In this essay I examine the aspects of liminality and initiation in Fellini’s films, using examples such as La strada, 8 and ½ and Amarcord as well as the broad context of all of his works and his biography.
Peut-on croire aux miracles sans offenser la raison?
Authors:   The Society of Theology and Thought, Guillaud Frédéric , 안 영주
Published: February 2023

L’objectif de cet article est d’examiner les deux arguments majeurs développés par les philosophes contre les miracles : l’argument de Spinoza, selon lequel les miracles sont métaphysiquement impossibles, et l’argument de Hume, selon lequel il est impossible de croire aux témoignages rapportant des miracles. L’examen de ces deux arguments est d’autant plus important qu’ils ont eu une importance considérable sur l’exégèse, en encourageant de nombreux savants à considérer tous les miracles comme des « mythes ». Il s’avère, à l’examen, que ces deux arguments ne sont pas solides, ce qui permet de lire à nouveau les Evangiles sans œillères.
The Historicity of Jesus’ Miracles a the Hermineutical Study of Miracles
Authors:   The Society of Theology and Thought, Baik Woon Chul
Published: February 2023

One of the most remarkable things about Jesus' actions is miracles. The four Gospels report healing, exorcism, raising the dead and performing many natural miracles. Are these miracle stories historically true? In modern times, commentators, such as Bultmann, who have been influenced by a mechanistic world-view, regard the miracle of Jesus as a myth or a product of imagination, created from apologetic motives. As a result of analyzing the texts by the historical-critical method, avoiding ideological prejudices, this paper reaches the conclusion that Jesus clearly performed the miracle of healing, exorcism, and raising the dead. Jesus declared that the kingdom of God has come upon you if I cast out demons by the finger of God. Likewise, through miracles, Jesus made the apocalyptic expectation of future salvation present.
A miracle is already a new heaven and a new earth on a small scale. With power they had received from Jesus, the disciples of the early church performed miracles that Jesus performed and they proclaimed the gospel of the kingdom of God. However, the miracle of Jesus' healing was reinterpreted by christological perspectives in the theology of the cross and resurrection, and the power of exorcism began to be replaced by baptism in the Holy Spirit. At thre same, this wonderful charismatic power gradually disappeared in the church,
How should Jesus' miracles be understood today? What are the challenges of today's scientific worldview to the miracles, and miracle faith? This paper presents a preliminary reflection on these topics as a conclusion.
An Ecotheological Consideration of creatio ex nihilo
Authors:   The Society of Theology and Thought, O'Hara Dennis Patrick
Published: February 2023

Accepting creation as a book of revelation along with scripture, the article examines the Magisterial teaching of creatio ex nihilo through an ecotheological lens. It reviews both the specific content of that teaching and its his-torical context before considering the correlations and disagreements between a cosmology of cosmogenesis and the teaching of creatio ex nihilo. After a close examination of the scriptural support for the teaching, the article returns to creation as a book of revelation to provide an ecotheological reflection on the teaching, suggesting how it might be updated given the passage of time and the advancement of our knowledge since its formulation.
Screening of MMP-2 Inhibiting Phytoconstituents for the Development of
Newer Pancreatic Cancer Treatment Modalities
Authors: Periyasamy Loganayaki , Murugantham Bharathi , Sundaraj Rajamanikandan , Krishnamoorthi Sneha , Muthusami Sridhar
Published: February 2023

Background:

Pancreatic cancer metastasis is characterized by a higher incidence of
morbidity and mortality. The present study attempts to identify phytocomponents with the potential to
inhibit the secretion of MMP-2 by pancreatic cancer cells and ascertain the efficacy of individual
components.

Methods:

Overall survival analysis carried out revealed reduced survival of patients with high MMP-2
expression. Data analysis from TCGA revealed increased MMP-2 expression in pancreatic cancer
patients compared to adjacent normal tissues. The expression of MMP-2 was reported at different
stages of pancreatic cancer (Stage I-IV). To understand the relevance of phytocomponents in binding
to the catalytic site of MMP-2, molecular docking studies were performed to find the effectiveness
based on Glide score/energy. To substantiate the in-silico analysis, the eight components were also
tested in vitro for reducing the survival in PANC-1 cells at three different time points (24, 48, and 72
hours). Finally, zymography analysis was performed using the eight components in the PANC-1
conditioned media of treated cells to ascertain the enzymatic activity of MMP-2.

Results:

The obtained results suggest plumbagin, emodin, and EGCG exert potential inhibition in
PANC-1 cells, among other phytocomponents tested. Therefore, as assessed using computational
studies, the binding ability of plumbagin, emodin, and EGCG can be interpreted as inhibiting effects
on MMP-2 activities.

Conclusion:

These compounds could find potential application in preventing the progression,
sustenance, and metastasis of pancreatic cancer and need to be explored further using a pre-clinical
model system in order to validate the efficacy, bioavailability, and safety.
Two-step Approach to Corrosion Detection of Metal Structures Using Convolutional Neural Networks When Inspecting Industrial Facilities
Authors: Rusakov K. D. , Chekhov A. V.
Published: February 2023

Purpose of research.Corrosion recognition on metal structures is a serious problem in conducting inspections of industrial facilities. Existing approaches to image analysis use all images to recognize areas damaged by corrosion, which is not suitable for structural analysis, since the percentage of errors in this approach is very large. Under conditions of corrosion prediction throughout the image, errors related to predictive mask not on metal structure are possible. Therefore, it is necessary to delete the results of positive class prediction for areas damaged by corrosion but not placed on metal structure. Therefore, in this work, the authors have developed two-step approach for recognizing corrosion of metal structures, thereby achieving the goal of improving recognition accuracy.Methods.We implement two deep learning models focused on Semantic segmentation (DeepLabv3, BiSeNetV2) for corrosion detection that work better in terms of accuracy and time and require fewer annotated samples compared to other deep models, such as Unet, FCN, Mask-RCNN. A new detection approach to metal areas damaged by corrosion, based on the combination of two convolutional neural networks for more accurate pixel prediction by depth architecture models: DeepLabv3 and BiSeNetV2.Results.Experimental studies have calculated the accuracy and F1 measures using FCN, Unet, Mask-RCNN models as well as the proposed approach. Based on obtained results, it was concluded that proposed approach of combining DeepLabv3 and BiSeNetV2 networks increases accuracy and F1 measure for Unet algorithm by 3%, accuracy by 10% and 2% F1 measure for Mask R-CNN and by 12% accuracy and 4% F1 measure for FCN network. Experimental results and comparisons with real data sets confirm the effectiveness of proposed scheme even for very complex images with many different defects. Productivity was assessed based on data annotated by experts.Conclusion.Analyses of existing solutions in the field of recognition of metal structures damaged by corrosion is described. Shortcomings of existing solutions based either on detection of corrosion sites or on pixel segmentation of full image are identified. A new approach to the recognition of metal areas damaged by corrosion based on the combination of two convolutional neural networks for more accurate pixel prediction of DeepLabv3 and BiSeNetV2 is indroduced. Production is evaluated based on data annotated by Precision and F1-score metrics experts.
Architectural and Structural Principles of Construction of Wooden Buildings over a Hundred Years Old
Authors: Stupishin L. Y. , Masalov A. V.
Published: February 2023

Purpose of research. Analysis of the accumulated information on design solutions used in the construction of buildings and structures made of wood, which ensured the safety of structural elements and materials, and allowing them to maintain their working condition for a long time. Study of the impact of operational impacts on building elements and development of recommendations for the restoration and preservation of timber buildings and structures classified as cultural heritage sites.Methods. Revealing the cause-and-effect relationships of the used design solutions and materials with the elimination of degradation processes in the supporting structures on the basis of office processing of the results of visual and instrumental examination.Results. We have collected data on the history, space-planning solution and constructive solution of the cultural heritage object. The cause-and-effect relationships of the protection of load-bearing structures from degradation phenomena in the materials of the structure by means of constructive and planning solutions for the building of the temple have been revealed.Conclusion. The building of the temple has a canonical space-planning solution, which allows maintaining a stable temperature, humidity and aeration mode of operation of the supporting structures of the building at any time of the year. The use of a rubble foundation made of phosphorite stone provides protection against uneven settlement of the building, as well as protects wooden structures from soil moisture. The phosphorite stone and the foundation binder are hydrophobic. A log-house technique has been used for erecting walls with a boarding (thin board) according to the type of a curtain facade, which provides ventilation of the log house. Ventilation of the floor, dome and its supporting structures are provided. The interior walls are decorated with currently unused materials that reliably protect the wood from atmospheric and biological harmful effects.
Chebyshev Alternance when Approximating Initial Conditions of the Inverse Cauchy Problem
Authors: Loktionov А. P.
Published: February 2023

Purpose of research. The work is devoted to a range of questions related to Cauchy problem on the segment of real axis with the application of the inverse Cauchy problem, in which real constants are initial conditions which are optimally restored according to experimental or tabular values of the solution of the differential equation. The object of the study is an information-measuring system, in which approximate values of initial conditions are calculated from discrete function values of Cauchy problem solving.Methods. The following problems are solved for this purpose: parameters of measuring section placement on the investigated object and approximation grid on measuring section are developed. Characteristics of recovery accuracy of initial conditions of the task are formulated.Results. An experimental-calculated method of determining initial conditions in the inverse Cauchy problem is proposed. It is based on the concept of objective function of regularization of the problem. Task regularization parameter in the form of minimum value by Lebesgue function is proposed.Conclusion. The reaction of uniformly approximating method of the initial conditions of the inverse Cauchy problem to the deviation of the approximation grid coordinates nodes from the coordinates of Chebyshev alternance was described. Graphs of method reaction to deviation of grid pitch from optimal pitch are given.
Investigation of the Process of Deep Drilling of Stainless Steel
Authors: Yatsun E. I. , Zubkova O. S. , Mogilev S. N.
Published: February 2023

Purpose of research. Experimental assessment of the effect of deep drilling modes for high-alloy steel on the components of cutting forces. Making holes in a solid material with a hole depth equal to or greater than ten hole diameters refers to deep drilling operations. For drilling, special tools are used – drills for deep drilling - ejector, cannon, gun drills. Universal or special equipment is applied, the tooling includes travelling or fixed rests, guide bushes. To achieve this goal, it is necessary to choose equipment: type of tool for deep drilling, type of setting; create a model of the forces acting on the cutting part of the tool during deep drilling; evaluate the degree of influence of processing modes on cutting forces.

Methods. The research is based on the cutting theory provisions, mechanical engineering technology, experiment planning and regression analysis.

Results. Based on the provisions of mechanical engineering technology, the setting up for deep drilling with a rotating workpiece and a non-rotating drill, which is imparted only by the axial feed, was developed. A cutting tool for performing a deep drilling operation is defined – a gun drill. A pattern was created for such a processing scheme and experimental cutting forces were calculated. According to the results of the calculations, it is determined that the greatest influence on the components of the cutting force in a given range of cutting modes is exerted by the axial feed rate of the tool.

Conclusion. The cutting forces that occur during deep drilling reach significant values. The polynomial equations obtained as a result of the study allow us to assess the degree of influence of processing modes on cutting forces, calculate cutting forces for further calculation for the sag of the stem of a gun drill.
Method for Early Diagnosis of Real-Time Traffic Loss with Timeout Monitoring in Software-Configurable Networks
Authors: Nikishin K. I.
Published: February 2023

The purpose of the research is to develop the method for early diagnosis of real-time traffic loss with time-out control in software defined networks (SDN).

Methods. An overview and description of the transfer of heterogeneous traffic in an Ethernet switch with support for quality of service and Time-Triggered Ethernet technology is given. The functioning of the timeout control blocks for instantaneous removal of a frame from the hard timeout control system and depending on the length of the frame when the idle timeout receiving side is reached is described in detail. These control units are included in the hardware guard of control time-outs for real-time traffic. The work of the switch OpenFlow is described using guard of control time-outs. The formation of time windows of timeouts during frame transmission is described and developed.

Results. With the help of the proposed method of early diagnosis of real-time traffic losses with control of timeouts in SDN, a modeling of SDN based on Petri nets and using the CPN Tools modeling package was carried out. The obtained model and experimental results are consistent with the theoretical calculations and the proposed method of transmitting real-time traffic, taking into account its losses in SDN.

Conclusion. Using the model, the functional and temporal behavior of the model was investigated; the method was verified using Petri nets. The results were obtained in the form of time diagrams reflecting the work on traffic types in accordance with the proposed method.
Practical Review on the Impact of Celiac Disease on the Patient
Authors: Ruddy Johannah , Scarlata Kate
Published: February 2023

Celiac disease (CD) is a multisystem immune-mediated disorder resulting in enteropathy of the small intestine with the ingestion of gluten, in genetically susceptible individuals. This condition impact 1% of the population and can result in the development of other conditions such as chronic fatigue, anemia, osteoporosis, aphthous stomatitis, elevated liver enzymes, joint pain, infertility, peripheral neuropathy, and epilepsy. In this review, we outline the clinical presentation of CD, the physiological differences between CD and non-celiac gluten sensitivity, proper management and the need for thorough patient education to increase adherence to a gluten free diet and reduce GI symptoms.
Contexto de cadena acuicultura y pesca
Authors: Zambrano Muñoz Adriana del Pilar
Published: February 2023

En esta ficha de contexto se mencionan los aspectos importantes de la cadena de acuicultura y pesca enfocados a su marco productivo, regional, económico, comercio internacional, así como sus perspectivas y tendencias frente al mercado, dando a conocer su entorno de Ciencia y Tecnología e Innovación.
No son "bichos"... son ¡insectos!
Authors:   ‎‎Corporación colombiana de investigación agropecuaria, Mosquera (Colombia)
Published: February 2023

Si todavía te refieres a "bichos" cuando hablas de insectos, necesitas ver este capítulo del Youtuber de agro.
Enfermedades del cultivo del cacao
Authors: Rodríguez Yzquierdo Gustavo , Martínez Botello Darwin , Posada Velásquez Luisa Fernanda
Published: February 2023

Este vídeo hace parte de una estrategia de transferencia de tecnología para diversificar la propagación de tecnología para diversificar la economía regional, contiene información sobre algunas enfermedades en el cultivo de cacao, así como algunas de sus causas, daños y maneras de enfrentarlas.
Proceso de evaluación de nuevas de caña de azúcar (Saccharum spp.) para la producción de panela: CC 93-7711 y CC 93-7510 de AGROSAVIA
Authors: López Lopera Juan Gonzalo , Barona Rodríguez Ayda Fernanda , Deantonio Florido Leidy Yibeth
Published: February 2023

En la agroindustria de la panela se presenta un bajo rendimiento de la caña, para enfrentar esta problemática la selección de nuevas variedades resulta de gran utilidad para el sector. Este proceso contribuye a; aumentar la cantidad de caña y panela por hectárea, buscar la tolerancia a plagas y enfermedades, lograr precocidad en los siclos de producción, obtener buena calidad de la panela, reducir costos de producción y conseguir la aceptación de los productores.
Unassisted Clinicians Versus Deep Learning–Assisted Clinicians in Image-Based Cancer Diagnostics: Systematic Review With Meta-analysis
Authors: Xue Peng , Si Mingyu , Qin Dongxu , Wei Bingrui , Seery Samuel , Ye Zichen , Chen Mingyang , Wang Sumeng , Song Cheng , Zhang Bo , Ding Ming , Zhang Wenling , Bai Anying , Yan Huijiao , Dang Le , Zhao Yuqian , Rezhake Remila , Zhang Shaokai , Qiao Youlin , Qu Yimin , Jiang Yu
Published: February 2023

Background

A number of publications have demonstrated that deep learning (DL) algorithms matched or outperformed clinicians in image-based cancer diagnostics, but these algorithms are frequently considered as opponents rather than partners. Despite the clinicians-in-the-loop DL approach having great potential, no study has systematically quantified the diagnostic accuracy of clinicians with and without the assistance of DL in image-based cancer identification.

Objective

We systematically quantified the diagnostic accuracy of clinicians with and without the assistance of DL in image-based cancer identification.

Methods

PubMed, Embase, IEEEXplore, and the Cochrane Library were searched for studies published between January 1, 2012, and December 7, 2021. Any type of study design was permitted that focused on comparing unassisted clinicians and DL-assisted clinicians in cancer identification using medical imaging. Studies using medical waveform-data graphics material and those investigating image segmentation rather than classification were excluded. Studies providing binary diagnostic accuracy data and contingency tables were included for further meta-analysis. Two subgroups were defined and analyzed, including cancer type and imaging modality.

Results

In total, 9796 studies were identified, of which 48 were deemed eligible for systematic review. Twenty-five of these studies made comparisons between unassisted clinicians and DL-assisted clinicians and provided sufficient data for statistical synthesis. We found a pooled sensitivity of 83% (95% CI 80%-86%) for unassisted clinicians and 88% (95% CI 86%-90%) for DL-assisted clinicians. Pooled specificity was 86% (95% CI 83%-88%) for unassisted clinicians and 88% (95% CI 85%-90%) for DL-assisted clinicians. The pooled sensitivity and specificity values for DL-assisted clinicians were higher than for unassisted clinicians, at ratios of 1.07 (95% CI 1.05-1.09) and 1.03 (95% CI 1.02-1.05), respectively. Similar diagnostic performance by DL-assisted clinicians was also observed across the predefined subgroups.

Conclusions

The diagnostic performance of DL-assisted clinicians appears better than unassisted clinicians in image-based cancer identification. However, caution should be exercised, because the evidence provided in the reviewed studies does not cover all the minutiae involved in real-world clinical practice. Combining qualitative insights from clinical practice with data-science approaches may improve DL-assisted practice, although further research is required.

Trial Registration

PROSPERO CRD42021281372; https://www.crd.york.ac.uk/prospero/display_record.php?RecordID=281372
Supporting Vulnerable Older Adults With Telehealth Through Wellness Calls and Tablet Distribution During COVID-19: Quality Improvement Project (Preprint)
Authors: Tewary Sweta , Cook Nicole , Dezine Marie , Shnayder Oksana , Pandya Naushira
Published: February 2023

BACKGROUND

Loneliness, social isolation, and lack of technical literacy are associated with poorer health outcomes. To help improve social connection during the COVID-19 pandemic, Nova Southeastern University’s South Florida Geriatric Workforce Enhancement Program partnered with a community-based organization to provide educational resources to promote telehealth services.

OBJECTIVE

This study aimed to provide educational resources to older adults with limited resources and promote the use of telehealth services in this population.

METHODS

Through this pilot project, we contacted 66 vulnerable older adults who expressed interest in telehealth support through wellness calls, with 44 participants moving on to participate in tablet usage. All tablets were preloaded with educational information on using the device, COVID-19 resources, and accessing telehealth services for patients, caregivers, and families.

RESULTS

Feedback from wellness assessments suggested a significant need for telehealth support. Participants used the tablets mainly for telehealth (n=6, 15%), to connect with friends and family (n=10, 26%), and to connect with faith communities (n=3, 8%).

CONCLUSIONS

The findings from the pilot project suggest that wellness calls and telehealth education are beneficial to support telehealth usage among older adults.