bluuebunny/crossref_metadata_2025 huggingface dataset:
Impacts of Biochar on Physical Properties and Erosion Potential of a Mudstone Slopeland Soil
Authors: Hseu Zeng-Yei , Jien Shih-Hao , Chien Wei-Hsin , Liou Ruei-Cheng
Published: December 2014

Food demand and soil sustainability have become urgent issues recently because of the global climate changes. This study aims to evaluate the application of a biochar produced by rice hull, on changes of physiochemical characteristics and erosion potential of a degraded slopeland soil. Rice hull biochar pyrolized at 400°C was incorporated into the soil at rates of 2.5%, 5%, and 10% (w/w) and was incubated for 168 d in this study. The results indicated that biochar application reduced the Bd by 12% to 25% and the PR by 57% to 92% after incubation, compared with the control. Besides, porosity and aggregate size increased by 16% to 22% and by 0.59 to 0.94 mm, respectively. The results presented that available water contents significantly increased in the amended soils by 18% to 89% because of the obvious increase of micropores. The water conductivity of the biochar-amended soils was only found in 10% biochar treatment, which might result from significant increase of macropores and reduction of soil strength (Bd and PR). During a simulated rainfall event, soil loss contents significantly decreased by 35% to 90% in the biochar-amended soils. In conclusion, biochar application could availably raise soil quality and physical properties for tilth increasing in the degraded mudstone soil.
Oral Verruciform Xanthoma: A Case Report and Literature Review
Authors: Marques Yonara Maria Freire Soares , de Andrade Cleverton Roberto , Machado de Sousa Suzana Cantanhede Orsini , Navarro Cláudia Maria
Published: December 2014

Oral verruciform xanthoma represents an uncommon entity, which affects mainly oral mucosa. This paper presents the major clinical and histological features of oral verruciform xanthoma and reports a case on the tongue. The differential diagnosis and a literature review are also provided in light of recent information.
Hypertension Education Intervention with Ugandan Nurses Working in Hospital Outpatient Clinic: A Pilot Study
Authors: Katende Godfrey , Groves Sara , Becker Kathleen
Published: December 2014

Noncommunicable diseases (NCDs) pose a significant global burden in both developed and developing countries. It is estimated that, by 2025, 41.7% of males and 38.7% of females in Sub-Saharan Africa will develop high blood pressure (HBP). This is particularly true in Uganda with hypertensive prevalence rates estimated to range from 22.5% to 30.5%. Coupled with low levels of detection, treatment, and control, hypertension represents a Ugandan public health crisis. An innovative WHO-ISH education program culturally was adapted in a pilot study and focused on knowledge, skills, and attitudes (KSA) of nurses caring for hypertensive patients in an outpatient clinic. Pre-post intervention data was collected and analyzed in which significant improvements were noted on all the three outcome measures. This pilot study demonstrated that nurses’ knowledge, skills, and attitudes could be significantly improved with a multimodal education program implemented in a low resource environment.
The Societal Impact of Herpes Zoster and Postherpetic Neuralgia on Patients, Life Partners, and Children of Patients in Germany
Authors: Weinke Thomas , Glogger Andrea , Bertrand Isabelle , Lukas Kati
Published: December 2014

The aim of this study was to assess the impact of herpes zoster (HZ) and postherpetic neuralgia (PHN) on the daily activities of patients and family members who care for them. Some former patients and family members participated in face-to-face interviews or in a T-group meeting (qualitative phase) and some participated in telephone interviews (quantitative phase). They all expressed feelings of helplessness and frustration mixed with depression, sadness, or rage. Many of the former patients said their lives stopped, in contrast to family members who said that their lives were busy and stressful. Family members caring for patients with PHN were more psychologically stressed than those caring for patients with HZ. Although former patients appreciated the psychological and emotional support given by their family members, they underestimated the impact that their disease had on them. Former patients and their family never forgot this illness and its considerable impact on their lives, particularly when PHN occurred. We need to raise the awareness of the general public about the real life impact of HZ and PHN and their often severe, debilitating consequences and the potential benefits from vaccination.
The Role of Metformin in Metabolic Disturbances during Pregnancy: Polycystic Ovary Syndrome and Gestational Diabetes Mellitus
Authors: Rojas Joselyn , Chávez-Castillo Mervin , Bermúdez Valmore
Published: December 2014

Maintenance of gestation implicates complex function of multiple endocrine mechanisms, and disruptions of the global metabolic environment prompt profound consequences on fetomaternal well-being during pregnancy and postpartum. Polycystic Ovary Syndrome (PCOS) and gestational diabetes mellitus (GDM) are very frequent conditions which increase risk for pregnancy complications, including early pregnancy loss, pregnancy-induced hypertensive disorders, and preterm labor, among many others. Insulin resistance (IR) plays a pivotal role in the pathogenesis of both PCOS and GDM, representing an important therapeutic target, with metformin being the most widely prescribed insulin-sensitizing antidiabetic drug. Although traditional views neglect use of oral antidiabetic agents during pregnancy, increasing evidence of safety during gestation has led to metformin now being recognized as a valuable tool in prevention of IR-related pregnancy complications and management of GDM. Metformin has been demonstrated to reduce rates of early pregnancy loss and onset of GDM in women with PCOS, and it appears to offer better metabolic control than insulin and other oral antidiabetic drugs during pregnancy. This review aims to summarize key aspects of current evidence concerning molecular and epidemiological knowledge on metformin use during pregnancy in the setting of PCOS and GDM.
Fractional Diffusion Equations for Lattice and Continuum: Grünwald-Letnikov Differences and Derivatives Approach
Authors: Tarasov Vasily E.
Published: December 2014

Fractional diffusion equations for three-dimensional lattice models based on fractional-order differences of the Grünwald-Letnikov type are suggested. These lattice fractional diffusion equations contain difference operators that describe long-range jumps from one lattice site to another. In continuum limit, the suggested lattice diffusion equations with noninteger order differences give the diffusion equations with the Grünwald-Letnikov fractional derivatives for continuum. We propose a consistent derivation of the fractional diffusion equation with the fractional derivatives of Grünwald-Letnikov type. The suggested lattice diffusion equations can be considered as a new microstructural basis of space-fractional diffusion in nonlocal media.
Identification of Plant Homologues of Dual Specificity Yak1-Related Kinases
Authors: Karpov Pavel , Raevsky Aleksey , Korablyov Maxim , Blume Yaroslav
Published: December 2014

Currently, Dual Specificity YAK1-Related Kinases (MNB/DYRK) were found in slime molds, protista, fungi, and animals, but the existence of plant homologues is still unclear. In the present study, we have identified 14 potential plant homologues with the previously unknown functions, based on the strong sequence similarity. The results of bioinformatics analysis revealed their correspondence to DYRK1A, DYRK1B, DYRK3, and DYRK4. For two plant homologues of animal DYRK1A from Physcomitrella patens and Arabidopsis thaliana spatial structures of catalytic domains were predicted, as well as their complexes with ADP and selective inhibitor d15. Comparative analysis of 3D-structures of the human DYRK1A and plant homologues, their complexes with the specific inhibitors, and results of molecular dynamics confirm their structural and functional similarity with high probability. Preliminary data indicate the presence of potential MNB/DYRK specific phosphorylation sites in such proteins associated with plant cytoskeleton as plant microtubule-associated proteins WVD2 and WDL1, and FH5 and SCAR2 involved in the organization and polarity of the actin cytoskeleton and some kinesin-like microtubule motor proteins.
Computed Tomography Features of Incidentally Detected Diffuse Thyroid Disease
Authors: Rho Myung Ho , Kim Dong Wook
Published: December 2014

Objective. This study aimed to evaluate the CT features of incidentally detected DTD in the patients who underwent thyroidectomy and to assess the diagnostic accuracy of CT diagnosis.Methods. We enrolled 209 consecutive patients who received preoperative neck CT and subsequent thyroid surgery. Neck CT in each case was retrospectively investigated by a single radiologist. We evaluated the diagnostic accuracy of individual CT features and the cut-off CT criteria for detecting DTD by comparing the CT features with histopathological results.Results. Histopathological examination of the 209 cases revealed normal thyroid (n=157), Hashimoto thyroiditis (n=17), non-Hashimoto lymphocytic thyroiditis (n=34), and diffuse hyperplasia (n=1). The CT features suggestive of DTD included low attenuation, inhomogeneous attenuation, increased glandular size, lobulated margin, and inhomogeneous enhancement. ROC curve analysis revealed that CT diagnosis of DTD based on the CT classification of “3 or more” abnormal CT features was superior. When the “3 or more” CT classification was selected, the sensitivity, specificity, positive and negative predictive values, and accuracy of CT diagnosis for DTD were 55.8%, 95.5%, 80.6%, 86.7%, and 85.6%, respectively.Conclusion. Neck CT may be helpful for the detection of incidental DTD.
Correlation of Ischemia-Modified Albumin with SOFA and APACHE II Scores in Preoperative Patients with Colorectal Cancer
Authors: Satoh Masaaki , Kotani Kazuhiko , Gugliucci Alejandro , Horie Hisanaga , Caccavello Russell , Takeuchi Mamoru
Published: December 2014

Purpose. Critical illnesses are assessed according to the sequential organ failure assessment (SOFA) and acute physiology and chronic health evaluation (APACHE) II. Circulating ischemia-modified albumin (IMA) is a biomarker generated under ischemic and oxidative conditions and may reflect disease severity in preoperative patients. This study investigated the correlations of IMA with SOFA and APACHE II scores in inpatients admitted for colorectal surgery.Methods. We examined 27 patients with advanced colorectal cancers (mean age 69 years, men/women = 15/12). Correlations between SOFA and APACHE II scores in addition to preoperative serum IMA and C-reactive protein (CRP) levels were analyzed.Results. The mean IMA level was 0.5 AU, and the median CRP level was 0.6 mg/dL. Median scores for SOFA and APACHE II were 2 and 12 points, respectively. Significant positive correlations between IMA and SOFA (r=0.45,P<0.05) and IMA and APACHE II (r=0.45,P<0.05) were identified which remained significant in confounder-adjusted analyses. In contrast, weak correlations were observed between CRP and the SOFA and APACHE II scores.Conclusions. The positive correlations between IMA and both SOFA and APACHE II scores suggest that serum IMA measurements reflect the severity of systemic failure in patients admitted for colorectal surgery in the preoperative phase.
A Regularization Process for Electrical Impedance Equation Employing Pseudoanalytic Function Theory
Authors: Robles Gonzalez Cesar Marco Antonio , Bucio Ramirez Ariana Guadalupe , Ponomaryov Volodymyr , Ramirez Tachiquin Marco Pedro
Published: December 2014

The electrical impedance equation is considered an ill‐posed problem where the solution to the forward problem is more easy to achieve than the inverse problem. This work tries to improve convergence in the forward problem method, where the Pseudoanalytic Function Theory by means of the Taylor series in formal powers is used, incorporating a regularization method to make a solution more stable and to obtain better convergence. In addition, we include a comparison between the designed algorithms that perform proposed method with and without a regularization process and the autoadjustment parameter for this regularization process.
Impairment of Sexual Life in Patients with Psoriasis
Authors: Sampogna Francesca , Gisondi Paolo , Tabolli Stefano , Abeni Damiano
Published: December 2014

Background: Sexual life can be severely affected in patients with psoriasis. However, this component is not often assessed by clinicians. Objective: To estimate the prevalence of sexual impairment and its degree of association with clinical and personal factors among psoriasis patients. Methods: Sexual life impairment was investigated in all eligible adults hospitalized with psoriasis in a dermatological hospital from February 2000 to February 2002, using answers to specific items from two dermatology-specific questionnaires, and from two psoriasis-specific questionnaires. Clinical severity and psychological problems were also evaluated. The same questionnaires were collected a month after hospital discharge, to estimate changes from baseline. Results: Of 936 patients,from 35.5% (Psoriasis Disability Index) to 71.3% (Impact of Psoriasis on Quality of Life Questionnaire) reported to have experienced sexual problems because of psoriasis. A more severe disease and the presence of psychological problems were also associated with sexual impairment. At 1-month follow-up, a reduction of >75% in clinical severity was associated with a twofold probability of substantial improvement of sexual life. Conclusions: Sexual impairment is very frequent in patients with psoriasis. Single questions on sexual life from quality of life questionnaires provide a simple tool that may be useful in clinical practice to assess sensitive issues, and thus to provide better patient-physician communication.
Impact of the Neuroendocrine System on Thymus and Bone Marrow Function
Authors: Barnard Adele , Layton Daniel , Hince Melanie , Sakkal Samy , Bernard Claude , Chidgey Ann , Boyd Richard
Published: December 2014

The nervous, endocrine, and immune systems interact to adapt to infection, inflammation, and tissue injury. Neural control is mediated in several ways, one of them being through the neuroendocrine regulation of the secretion of hypothalamic and pituitary hormones. The hormonal effects on the immune system range from the impact of steroidal hormones, which exhibit inhibitory effects over immune functions, to growth hormone, prolactin and neurohypophyseal hormones, known to stimulate and modulate humoral and cellular aspects of the immune system. This review will discuss the mechanisms behind the immunomodulatory role of the neuroendocrine system, including the critically important feedback loops required to maintain balance for these bidirectional interactions and alterations that occur with age.
Management of the Cardiorenal Syndrome in Decompensated Heart Failure
Authors: Verbrugge Frederik Hendrik , Grieten Lars , Mullens Wilfried
Published: December 2014

Background: The management of the cardiorenal syndrome (CRS) in decompensated heart failure (HF) is challenging, with high-quality evidence lacking. Summary: The pathophysiology of CRS in decompensated HF is complex, with glomerular filtration rate (GFR) and urine output representing different aspects of kidney function. GFR depends on structural factors (number of functional nephrons and integrity of the glomerular membrane) versus hemodynamic alterations (volume status, renal perfusion, arterial blood pressure, central venous pressure or intra-abdominal pressure) and neurohumoral activation. In contrast, urine output and volume homeostasis are mainly a function of the renal tubules. Treatment of CRS in decompensated HF patients should be individualized based on the underlying pathophysiological processes. Key Messages: Congestion, defined as elevated cardiac filling pressures, is not a surrogate for volume overload. Transient decreases in GFR might be accepted during decongestion, but hypotension must be avoided. Paracentesis and compression therapy are essential to remove fluid overload from third spaces. Increasing the effective circulatory volume improves renal function when cardiac output is depressed. As mechanical support is invasive and inotropes are related to increased mortality, afterload reduction through vasodilator therapy remains the preferred strategy in patients who are normo- or hypertensive. Specific therapies to augment renal perfusion (rolofylline, dopamine or nesiritide) have rendered disappointing results, but recently, serelaxin has been shown to improve renal function, even with a trend towards reduced all-cause mortality in selected patients. Diuretic resistance is associated with worse outcomes, independent of the underlying GFR. Combinational diuretic therapy, with ultrafiltration as a bail-out strategy, is indicated in case of diuretic resistance. i 2014 S. Karger AG, Basel
A Randomized Control Study on the Procedure for Switching Epoetin Beta (EPO) to Epoetin Beta Pegol (CERA) in the Treatment of Renal Anemia in Maintenance Hemodialysis Patients
Authors: Toida Tatsunori , Sato Yuji , Shibata Norihiko , Kitamura Kazuo , Fujimoto Shouichi
Published: December 2014

Background/Aims: We investigated the method of switching EPO to CERA that does not cause a decrease in the Hb level. Methods: Fifty EPO-treated patients were randomly divided into two groups in which CERA was administered every two weeks (Q2W) or every four weeks (Q4W). After 8 weeks of treatment, the frequency of administration was changed to Q4W in the former. Follow-up was performed for 24 weeks. Results: There was no difference in the Hb level between the two groups until 6 weeks. In the Q2W group, the Hb maintained a stable level throughout a study period. However, in the Q4W group, the Hb level was significantly lower than in the Q2W group at weeks 9, 11, and 13. Conclusion: EPO switching to CERA without a decrease in the Hb level could be achieved by administering CERA every two weeks, but not every four weeks, for a specific period after switching. © 2014 S. Karger AG, Basel
Potential Impact of Air Pollution on Multiple Sclerosis in Tehran, Iran
Authors: Heydarpour Pouria , Amini Hassan , Khoshkish Shayan , Seidkhani Hossein , Sahraian Mohammad Ali , Yunesian Masud
Published: December 2014

Background: Multiple sclerosis (MS) incidence has dramatically increased in Tehran, Iran. The health impact of air pollution in Tehran underscores the attention to a possible association to this environmental risk factor. In this study, the authors aimed to analyze the spatial distribution of prevalent MS cases and their association with the spatial patterns of air pollution. Methods: Patient records meeting McDonald's criteria for definite MS diagnosis with disease onset during 2003-2013 were obtained. Next, the location of 2,188 patients was successfully geo-referenced within Tehran metropolis by geographic information system (GIS) bureau of Iran's post office based on their phone numbers. A cluster analysis was performed using the average nearest neighbor index (ANNI) and quadrat analysis. The long-term exposures of MS patients to particulate matter (PM10), sulfur dioxide (SO2), nitrogen oxide (NO), nitrogen dioxide (NO2), and nitrogen oxides (NOx) were estimated using the previously developed land use regression models. Results: Prevalent MS cases had a clustered pattern in Tehran. A significant difference in exposure to PM10, SO2, NO2, and NOx (p < 0.001) was observed in MS cases compared with controls. Conclusion: This study revealed the potential role of long-term exposure to air pollutants as an environmental risk factor in MS.
Kisspeptin-Gpr54 Signaling at the GnRH Neuron Is Necessary for Negative Feedback Regulation of Luteinizing Hormone Secretion in Female Mice
Authors: Yeo Shel-Hwa , Clarkson Jenny , Herbison Allan E.
Published: December 2014

Kisspeptin-Gpr54 signaling is critical for regulating the activity of gonadotropin-releasing hormone (GnRH) neurons in mammals. Previous studies have shown that the negative feedback mechanism is disrupted in global Gpr54-null mutants. The present investigation aimed to determine (1) if a lack of cyclical estrogen exposure of the GnRH neuronal network in the life-long hypogonadotropic Gpr54-null mice contributed to their failed negative feedback mechanism and (2) the cellular location of disrupted kisspeptin-Gpr54 signaling. Plasma luteinizing hormone (LH) concentrations were determined in individual adult female mice when intact, following ovariectomy (OVX) and in response to an acute injection of 17β-estradiol (E2). Control mice exhibited a characteristic rise in LH after OVX that was suppressed by acute E2. Global Gpr54-null mice failed to exhibit any post-OVX increase in LH or response to E2. Adult female global Gpr54-null mice given a cyclical regimen of estradiol for three cycles prior to OVX also failed to exhibit any post-OVX increase in LH or response to E2. To address whether Gpr54 signaling at the GnRH neuron itself was necessary for the failed response to OVX in global Gpr54-null animals, adult female mice with a GnRH neuron-selective deletion of Gpr54 were examined. These mice also failed to exhibit any post-OVX increase in LH or response to E2. These experiments demonstrate defective negative feedback in global Gpr54-null mice that cannot be attributed to a lack of prior exposure of the GnRH neuronal network to cyclical estradiol. The absence of negative feedback in GnRH neuron-selective Gpr54-null mice demonstrates the necessity of direct kisspeptin signaling at the GnRH neuron for this mechanism to occur.
Intradialytic Exercise in Hemodialysis Patients: A Systematic Review and Meta-Analysis
Authors: Sheng Kaixiang , Zhang Ping , Chen Lili , Cheng Jun , Wu Congcong , Chen Jianghua
Published: December 2014

Background and Objective: Hemodialysis (HD) patients are more inactive, leading to poor functional capacity and quality of life; this may be reversed with intradialytic exercise training. To systematically evaluate the efficacy and safety of intradialytic exercise for HD patients, we conducted a meta-analysis of the published randomized controlled trials. Data Sources and Methods: Medline, Embase, and Cochrane Central Register of Controlled Trials were systematically searched up to February, 2014. The reference lists of eligible studies and relevant reviews were also checked. Results: 24 studies of 997 patients were included. Compared with control, intradialytic exercise significantly improve Kt/V (SMD = 0.27, 95% CI 0.01-0.53), peak oxygen consumption (VO2peak) (SMD = 0.53, 95% CI 0.30-0.76), and physical performance of physical function of life (SMD = 0.30, 95% CI 0.04-0.55). However, no significant improvements were found in the mental function of life. There was no significant difference with respect to musculoskeletal and cardiovascular complications between the intradialytic exercise groups and control groups. Further subgroup analysis found that, when the trial duration was more than 6 months, the intervention had significant effects on VO2peak (SMD = 0.89, 95% CI 0.56-1.22). However, when the trial duration was less than 6 months, the change of VO2peak was not significant (SMD = 0.19, 95% CI -0.13 to 0.51). Conclusion: Intradialytic exercise can improve Kt/V, VO2peak, and the physical quality of life, and intradialytic exercise is safe for HD patients. Therefore, we put forward the suggestion that clinical guideline be updated to inform clinicians on the benefits of intradialytic exercise on HD patients. i 2014 S. Karger AG, Basel
Cytokine Removal with High Cut-Off Membrane: Review of Literature
Authors: Villa Gianluca , Zaragoza Jose J. , Sharma Aashish , Neri Mauro , De Gaudio A. Raffaele , Ronco Claudio
Published: December 2014

During the last decade, blood purification therapies have been proposed as an effective therapy to control the cytokines dysregulation in systemic inflammatory syndromes. Among them, the treatment with high cut-off membranes is characterized by larger pore size and more effective clearance for middle molecular weight molecules (cytokines). In this paper, we performed a thoughtful review of the literature on HCO being used for blood purification indications in all systemic inflammation syndromes. Clinical and experimental studies show that the use of high effluent flows in a pure diffusive treatment effectively removes serum cytokines with a safe profile in albumin clearance. In clinical studies, the removal of these inflammatory mediators is associated with a significant improvement in hemodynamic condition, oxygenation indices, and organ dysfunction. © 2014 S. Karger AG, Basel
Environmental Stressors and Epigenetic Control of the Hypothalamic-Pituitary-Adrenal Axis
Authors: Lee Richard S. , Sawa Akira
Published: December 2014

In this review, we provide a brief summary of several key studies that broaden our understanding of stress and its epigenetic control of the function and behavior of the hypothalamic-pituitary-adrenal (HPA) axis. Clinical and animal studies suggest a link among exposure to stress, dysregulation of the HPA axis, and susceptibility to neuropsychiatric illnesses. Recent studies have supported the notion that exposure to glucocorticoids and stress in various forms, durations, and intensities during different periods of development leads to long-lasting maladaptive HPA axis response in the brain. They demonstrate that this maladaptive response is comprised of persistent epigenetic changes in the function of HPA axis-associated genes that govern homeostatic levels of glucocorticoids. Stressors and/or disruption of glucocorticoid dynamics also target genes such as brain-derived neurotrophic factor(BDNF) and tyrosine hydroxylase(TH) that are important for neuronal function and behavior. While a definitive role for epigenetic mechanisms remains unclear, these emerging studies implicate glucocorticoid signaling and its ability to alter the epigenetic landscape as one of the key mechanisms that alter the function of the HPA axis and its associated cascades. We also suggest some of the requisite studies and techniques that are important, such as additional candidate gene approaches, genome-wide epigenomic screens, and innovative functional and behavioral studies, in order to further explore and define the relationship between epigenetics and HPA axis biology. Additional studies examining stress-induced epigenetic changes of HPA axis genes, aided by innovative techniques and methodologies, are needed to advance our understanding of this relationship and lead to better preventive, diagnostic, and corrective measures.
GnRH Neurons of Young and Aged Female Rhesus Monkeys Co-Express GPER but Are Unaffected by Long-Term Hormone Replacement
Authors: Naugle Michelle M. , Gore Andrea C.
Published: December 2014

Menopause is caused by changes in the function of the hypothalamic-pituitary-gonadal axis that controls reproduction. Hypophysiotropic gonadotropin-releasing hormone (GnRH) neurons in the hypothalamus orchestrate the activity of this axis and are regulated by hormonal feedback loops. The mechanisms by which GnRH responds to the primary regulatory sex steroid hormone, estradiol (E2), are still poorly understood in the context of menopause. Our goal was to determine whether the G protein-coupled estrogen receptor (GPER) is co-expressed in adult primate GnRH neurons and whether this changes with aging and/or E2 treatment. We used immunofluorescence double-labeling to characterize the co-expression of GPER in GnRH perikarya and terminals in the hypothalamus. Young and aged rhesus macaques were ovariectomized and given long-term (∼2-year) hormone treatments (E2, E2 + progesterone, or vehicle) selected to mimic currently prescribed hormone replacement therapies used for the alleviation of menopausal symptoms in women. We found that about half of GnRH perikarya co-expressed GPER, while only about 12% of GnRH processes and terminals in the median eminence (ME) were double-labeled. Additionally, many GPER-labeled processes were in direct contact with GnRH neurons, often wrapped around the perikarya and processes and in close proximity in the ME. These results extend prior work by showing robust co-localization of GPER in GnRH in a clinically relevant model, and they support the possibility that GPER-mediated E2 regulation of GnRH occurs both in the soma and terminals in nonhuman primates.
Differentiation of Pluripotent Stem Cells into Hypothalamic and Pituitary Cells
Authors: Suga Hidetaka
Published: December 2014

The hypothalamic-pituitary system is essential to maintain life and control systemic homeostasis, but it is negatively affected by various diseases, leading to serious symptoms. Embryonic stem (ES) cells differentiate into neuroectodermal progenitors when cultured as floating aggregates under serum-free conditions. Recently, our colleagues have shown that strict removal of exogenous patterning factors during early differentiation steps induced efficient generation of rostral hypothalamic-like progenitors from mouse ES cell-derived neuroectodermal cells. The use of growth factor-free chemically defined medium was critical for this induction. The ES cell-derived hypothalamic-like progenitors generated rostral-dorsal hypothalamic neurons, especially magnocellular vasopressinergic neurons that release the hormone upon stimulation. Subsequently, we reported efficient self-formation of 3-dimensional adenohypophysis tissues in aggregate cultures of mouse ES cells. The ES cells were stimulated to differentiate into nonneural head ectoderm and hypothalamic neuroectoderm in adjacent layers within the aggregate and then treated with hedgehog. Self-organization of Rathke's pouch-like structures occurred at the interface of the two epithelia, as observed in vivo, and various endocrine cells including corticotrophs and somatotrophs were subsequently produced. The corticotrophs efficiently secreted adrenocorticotropic hormone in response to corticotropin-releasing hormone. Furthermore, when engrafted in vivo, these cells rescued the systemic glucocorticoid level in hypopituitary mice. Our present research aims are to prepare hypothalamic and pituitary tissues from human induced pluripotent stem cells and establish effective transplantation techniques with clinical applications. To replicate the complex and precise control of the hypothalamic-pituitary system, regenerative medicine using pluripotent cells may be a hopeful option.
The Role of Tumor Necrosis Factor Alpha in Regulating the Expression of Tamm-Horsfall Protein (Uromodulin) in Thick Ascending Limbs during Kidney Injury
Authors: Heitmeier Monique , McCracken Ruth , Micanovic Radmila , Khan Shehnaz , El-Achkar Tarek M.
Published: December 2014

Background: Tamm-Horsfall Protein (THP) is a glycoprotein expressed exclusively by cells of the thick ascending loop (TAL) of Henle. THP has a protective role in acute kidney injury (AKI), and its expression is downregulated in the early stages of injury. Tumor necrosis factor alpha (TNFE) is a cytokine endogenously expressed by the TAL and is also induced by AKI. Therefore, we hypothesized that TNFE is a key regulator of THP expression. Methods: We used a mouse model of AKI (ischemia-reperfusion injury, IRI) and a cell culture system of a TAL cell line (MKTAL). Results: We show that TNFE is upregulated by TAL cells early after AKI in vivo. The expression of THP and its transcription factor Hepatocyte nuclear factor 1F (HNF1F) were concomitantly decreased at the peak of injury. Furthermore, recombinant TNFE inhibits significantly, and in a dose-dependent manner, the expression of THP, but not HNF1F in MKTAL cells. Interestingly, neither TNFE neutralization nor genetic deletion of TNFE increased THP or HNF levels after injury in vivo. Conclusion: Our data suggest that TNFE can inhibit the expression of THP in TAL cells via an HNF1F-independent mechanism, but the downregulation of THP expression in the early AKI does not depend on TNFE. We propose that TNFE regulates THP expression in a homeostatic setting, but the impact of TNFE on THP during kidney injury is superseded by other factors that could inhibit HNF1F-mediated expression of THP. i 2014 S. Karger AG, Basel
Transplant Nephrectomy: Histologic Findings - A Single Center Study
Authors: Goral Simin , Brukamp Kirsten , Ticehurst Erin H. , Abt Peter L. , Bloom Roy D. , Kearns Jane , Constantinescu Serban , Kamoun Malek , Tomaszewski John
Published: December 2014

Aims: To identify the histopathological features of transplant nephrectomy (TN) specimens. Methods: We performed retrospective analysis of 73 nephrectomies to review the histopathology in detail and correlate the Banff grading characteristics of TN specimens with time post engraftment and clinical features. Retrospective data on donor-specific antibodies (DSA) were also collected. Results: The majority of patients who had TN in less than 3 months posttransplant (n = 20; median time to TN: 4 days) had hemorrhagic infarction; 7 patients (35%) had grade 3 acute rejection (AR). Patients who had TN later than 3 months posttransplant (n = 53; median time to TN: 67 months) had AR, grade 2B (21%) and 3 (43%), coexisting with advanced vascular injury in the form of interstitial hemorrhage, extensive interstitial fibrosis and tubular atrophy (IF/TA) as well as the presence of DSAs. Overall, the majority of patients without DSA pre-TN developed DSA post-TN. Conclusions: Our data revealed extensive inflammation and ongoing immunologic activity in a subset of patients with a failed graft. Careful and individualized approach based on clinical and laboratory data should guide the decision for transplant nephrectomy. i 2014 S. Karger AG, Basel
Beyond the Conflict Between ‘Reason’ and ‘Revelation’
Authors: Sherwood Yvonne
Published: December 2014

In De veritate, sacrifice is appealed to as a universal rite and the ultimate guarantee of immutable truth, beyond reasonable deduction or natural instinct (Book 1, Chapter 7; cf. De satisfactione Christi). But sacrifice also stands as the ultimate example of the abrogation and alteration of law (Book 5, chs. 6–8). As an example of the abrogation of law, sacrifice signifies in both directions. The case of Abraham (Genesis 22) demonstrates God’s sovereign power of dispensatio. Divine right to radical revision is demonstrated in the command to sacrifice. But more generally it is the suspension of the command to sacrifice that stands as the ultimate sign of sovereign right not just to annotate but to radically rewrite the law. In this paper I explore how sacrifice operates as a guarantee of immutability and mutability: the intractability of scripture, and its equally necessary revision and alteration. Sacrifice reaches across all time and space, and stands as a sign of the parochialisation of biblical time and space. This tension relates to the principle of accommodation which, I argue, is already in operation in the Bible. By extrapolating this fundamentally biblical operation, Grotius produces a paradox that will help to sustain the Bible in modernity. The Bible (as emblematised in sacrifice) is localised and parochialised but also persists as a ‘universal’ foundation.
Three new species of Ficus (Moraceae) from Central and northern-South America
Authors: Medina Nicolás
Published: December 2014

Three new species of Ficus are described and illustrated. F. nebulosilvana which is restricted to the cloud forest of western Andes in Colombia and Ecuador, F. palmarensis which is restricted to the cloud forests of Chocó in Colombia, and F. plectonervata which is distributed from the central biogeographic region of Chocó in Colombia to Costa Rica. Species conservation assessments are presented and the taxonomic relationships of the new species and etymology are discussed.
Redescription and molecular characterisation of Dujardinascaris madagascariensis and a note on D. dujardini (Nematoda: Heterocheilidae), parasites of Crocodylus niloticus, with a key to Dujardinascaris spp. in crocodilians
Authors: MAŠOVÁ ŠÁRKA , BARUŠ VLASTIMIL , SEIFERTOVÁ MÁRIA , MALALA JOHN , JIRKŮ MILOSLAV
Published: December 2014

An examination of one specimen of Nile crocodile, Crocodylus niloticus (Laurenti, 1768), from Lake Turkana (Kenya), revealed the presence of two ascaridoid nematodes belonging to the genus Dujardinascaris Baylis, 1947. Dujardinascaris madagascariensis Chabaud & Caballero, 1966 was studied by scanning electron microscopy, redescribed, and differentiated from D. dujardini (Travassos, 1920). Dujardinascaris madagascariencsis is the second of the genus to be sequenced. An internal fragment of the small ribosomal subunit and nuclear ribosomal DNA internal transcribed spacer 2 region were amplified—the slowly evolving 18S gene region was used for phylogenetic analysis. Molecular data confirmed affinity of D. madagascariensis to the family  Heterocheilidae and revealed its closest relationship with D. waltoni. A key to the species of Dujardinascaris parasitizing crocodiles is provided.
Two new species of the genus Dasybasis Macquart, 1847, from Chile (Diptera: Tabanidae: Diachlorini)
Authors: GONZÁLEZ CHRISTIAN R.
Published: December 2014

The females of Dasybasis antillanca and Dasybasis collagua, new species, are described from specimens collected in Osorno and Iquique Provinces, south and north Chile, respectively. Their relationships to other Dasybasis species are discussed.
The Cylindrobulla / Ascobulla complex—unraveling problems in identification and adding to Cylindrobulla diversity (Gastropoda, Heterobranchia, Sacoglossa) by describing a new species
Authors: LAETZ ELISE , CHRISTA GREGOR , HÄNDELER KATHARINA , WÄGELE HEIKE
Published: December 2014

Sacoglossa (Gastropoda: Heterobranchia) are generally considered a monophyletic group, previously associated within the now defunct “Opisthobranchia”, but now basally located within Panpulmonata. In the light of this new phylogenetic hypothesis, detailed knowledge of the most basal groups within Sacoglossa is of paramount importance. This study focuses on the genus Cylindrobulla, which is usually considered the most basal group within the Sacoglossa from a morphological point of view, because it does not share the typical elongate radula teeth of all other Sacoglossa. We describe a new species, Cylindrobulla schuppi sp. nov., and provide data on its food. We reexamined and clarify the radula of the type species C. beauii, review the genus with all other valid species, provide new characters to aid in the proper identification of species within this genus, compare it to the very similar genus Ascobulla, present a determination key using external characters to ensure proper identification of the two similar genera, and discuss phylogenetic relationships within the shelled sacoglossan, the Oxynoacea.
A new species of Eostyloceros (Cervidae, Artiodactyla) from the Late Miocene of the Linxia Basin in Gansu, China
Authors: DENG TAO
Published: December 2014

A new species, Eostyloceros hezhengensis sp. nov., is established based on a skull with its cranial appendages collected from the Late Miocene Liushu Formation of the Linxia Basin in Gansu Province, northwestern China. It is a large-sized muntjak with a distinct longitudinal ridge along the lateral margin of the frontal bone that joins the antler pedicle. The pedicle is short, cylindrical, robust, and extends posteriorly from the rear of the orbit. The anterior and posterior branches arise from the burr and diverge at an angle of 30°. The posterior branch is relatively long, and its tip is strongly curved posteriorly. The anterior branch is straight and situated anteromedially from the posterior branch. The posterior branch is lateromedially compressed, and the anterior branch has a circular cross section. The morphological observation together with a cladistic and a principal component analysis indicate that E. hezhengensis is more basal than any known species of the genus Eostyloceros in having shorter pedicles, a lower position of the fork above the burr, more slender anterior branches, and a small angle between the anterior and posterior branches. Its age is the middle Late Miocene, corresponding to the late Bahean.
The immature stages of Polypedilum (Pentapedilum) nodosum (Johannsen) and Polypedilum (Tripodura) masudai (Tokunaga) (Diptera, Chironomidae, Chironominae)
Authors: TANG HONG-QU , CRANSTON PETER S. , ZHAO JIAN-GANG , LOK CHAN-WA , WONG KAI-CHIN , LI ZHI-QIANG
Published: December 2014

Based on associated material collected from Macau and Guangxi, the pupae and larvae of Polypedilum (Pentapedilum) nodosum Johannsen and P. (Tripodura) masudai Tokunaga are described completely for the first time. Both species are newly recorded from China. Characters for distinguishing the immature stage of these species from other allied Polypedilum species are noted. The previously recorded Polypedilum (Pentapedilum) ‘K1’ of Cranston  (1996) is shown to be P. nodosum. Information is provided on distribution and ecological tolerances.
A survey of the Cape Floristic Region of South Africa for the presence of cyst nematodes (Nematoda: Heteroderidae)
Authors: Knoetze Rinus , Swart Antoinette
Published: December 2014

A survey was performed to detect the presence of cyst nematodes in the Cape Floristic Region of South Africa. Soil was collected in the rhizosphere of the dominant plant species within blocks of indigenous vegetation and cysts were extracted from them. A total of 81 blocks of indigenous vegetation were sampled as described. Cysts were detected in 7 of these samples, representing 6 different vegetation types. One set of primers was used to amplify the ITS regions from these cysts, including the 5.8S ribosomal gene, as well as short parts of the 18S and 28S ribosomal genes. ITS-rDNA sequences from the indigenous isolates were aligned with selected sequences of other species from the Heteroderidae. Phylogenetic analyses to resolve the relationships between indigenous isolates and selected representatives of the Heteroderidae were conducted using the Maximum Parsimony method. The consensus tree resulting from alignment of the circumfenestrate cysts revealed that isolates SK18, WK1 and WK26 are included in a clade of Globodera species that parasitise non-solanaceous plants, forming a monophyletic group with G. millefolii, G. artemisiae, and an unidentified Globodera sp. from Portugal. In a tree resulting from the alignment of the Heterodera spp., isolates OK14 and WK2 are included in the Afenestrata group, forming a monophyletic group with H. orientalis.This survey unearthed at least four potentially new species of cyst nematodes, which may prove invaluable for the study of the evolution and biogeography of the group.
A new species of the genus Mygdonia (Hemiptera: Heteroptera: Coreidae)
from Madagascar with a key to species
Authors: Baňař PETR , BRAILOVSKY Harry , HUBÁČKOVÁ LENKA , HEMALA VLADIMÍR
Published: December 2014

A new species, Mygdonia milivoji sp. nov. (Coreidae: Coreinae: Mictini) from south-west Madagascar is described, illustrated and compared with widely distributed M. elongata Distant, 1879. A key to Mygdonia species based on females is given and distribution of both Madagascan species is briefly discussed.
Diverse Influences of ENSO on the East Asian–Western Pacific Winter Climate Tied to Different ENSO Properties in CMIP5 Models
Authors: Gong Hainan , Wang Lin , Chen Wen , Nath Debashis , Huang Gang , Tao Weichen
Published: December 2014

The influence of El Niño–Southern Oscillation (ENSO) on the East Asian–western Pacific (EAWP) climate in boreal winter is investigated in the phase 5 of the Coupled Model Intercomparison Project (CMIP5) model results and then compared to that in the phase 3 (CMIP3) results. In particular, the role played by the differences among models in ENSO properties, including the amplitude and longitudinal extension of ENSO’s sea surface temperature (SST) pattern, is analyzed. Results show that an eastward shrinking of ENSO’s SST pattern leads to quite weak circulation and climatic responses over the EAWP regions in the models. On the contrary, a westward expansion of the SST pattern shifts the anomalous Walker circulation too far west. The resultant precipitation anomalies and lower-tropospheric atmospheric Rossby wave responses both extend unrealistically into the Indian Ocean, and the hemispheric asymmetry of the Rossby wave response is missing. All these features lead to unrealistic climatic impacts of ENSO over the EAWP regions. In contrast to the above two cases, a reasonable longitudinal extension of ENSO’s SST pattern corresponds to better ENSO teleconnections over the EAWP regions. Nevertheless, the atmospheric responses over the western Pacific are still located farther west than observed, implying a common bias of CMIP5 models. In this case, a larger amplitude of ENSO variability to some extent helps to reduce model biases and facilitate better climatic responses to ENSO in the EAWP regions. Compared with CMIP3 models, CMIP5 models perform better in representing ENSO’s impacts on the East Asian winter climate.
Enhancing Model Skill by Assimilating SMOPS Blended Soil Moisture Product into Noah Land Surface Model
Authors: Yin Jifu , Zhan Xiwu , Zheng Youfei , Liu Jicheng , Fang Li , Hain Christopher R.
Published: December 2014

Many studies that have assimilated remotely sensed soil moisture into land surface models have generally focused on retrievals from a single satellite sensor. However, few studies have evaluated the merits of assimilating ensemble products that are merged soil moisture retrievals from several different sensors. In this study, the assimilation of the Soil Moisture Operational Products System (SMOPS) blended soil moisture (SBSM) product, which is a combination of soil moisture products from WindSat, Advanced Scatterometer (ASCAT), and Soil Moisture and Ocean Salinity (SMOS) satellite sensors is examined. Using the ensemble Kalman filter (EnKF), a synthetic experiment is performed on the global domain at 25-km resolution to assess the impact of assimilating the SBSM product. The benefit of assimilating SBSM is assessed by comparing it with in situ observations from U.S. Department of Agriculture Soil Climate Analysis Network (SCAN) and the Surface Radiation Budget Network (SURFRAD). Time-averaged surface-layer soil moisture fields from SBSM have a higher spatial coverage and generally agree with model simulations in the global patterns of wet and dry regions. The impacts of assimilating SMOPS blended data on model soil moisture and soil temperature are evident in both sparsely and densely vegetated areas. Temporal correlations between in situ observations and net shortwave radiation and net longwave radiation are higher with assimilating SMOPS blended product than without the data assimilation.