bluuebunny/crossref_metadata_2025 huggingface dataset:
Assessing Facial Symmetry and Attractiveness using Augmented Reality
Authors: Wei Wei , Ho Edmond S. L. , McCay Kevin D. , Damaševičius Robertas , Maskeliūnas Rytis , Esposito Anna
Published: March 2021

Facial symmetry is a key component in quantifying the perception of beauty. In this paper, we propose a set of facial features computed from facial landmarks which can be extracted at a low computational cost. We quantitatively evaluated the proposed features for predicting perceived attractiveness from human portraits on four benchmark datasets (SCUT-FBP, SCUT-FBP5500, FACES and Chicago Face Database). Experimental results showed that the performance of the proposed features is comparable to those extracted from a set with much denser facial landmarks. The computation of facial features was also implemented as an augmented reality (AR) app developed on Android OS. The app overlays four types of measurements and guidelines over a live video stream, while the facial measurements are computed from the tracked facial landmarks at run time. The developed app can be used to assist plastic surgeons in assessing facial symmetry when planning reconstructive facial surgeries.
Garry oak ecosystem stand history in Southwest British Columbia, Canada: implications of environmental change and indigenous land use for ecological restoration and population recovery
Authors: Barlow Celeste M. , Pellatt Marlow G. , Kohfeld Karen E.
Published: March 2021

In the Pacific Northwest of North America, endangered Garry oak ecosystems have a complex history that integrates effects of Holocene climate change, Indigenous land management, and colonial settlement during the Anthropocene. In western Canada, Garry oak and Douglas fir recruitment corresponds with the end of the Little Ice Age (LIA; ca. 1870), after the collapse of Indigenous populations but in some cases prior to European settlement. We examined establishment patterns at three sites in southwest British Columbia, each with different edaphic characteristics based on slope, exposure, and drainage. At our Somenos Marsh site on Vancouver Island, we see a clear relationship between Indigenous occupation, subsequent European settlement, and development of an oak woodland, indicating that Indigenous land management was important for development of many Garry oak ecosystems. However, at the Tumbo Cliff site (Tumbo Island, BC), shallow soil xeric conditions, regional climate, and periodic fire were likely drivers of stand and ecosystem development. Finally, at the deep soil Tumbo Marsh site, Garry oak established and grew quickly when conditions were favorable, following the early twentieth century conversion of a saltwater tidal flat into a freshwater marsh. Combining site level historical records, site characteristics, and dendrochronological data provides a greater understanding of the local and regional factors that shape the unique structures of Garry oak ecosystems at each site. This information can be integrated into restoration and fire management strategies for Garry oak ecosystems as well as elucidate the timing of European settler and climate change impacts on these ecosystems.
Evaluation of the role of staphylococci in the pathomechanism of conjunctivitis
Authors: Jasińska Ewa , Bogut Agnieszka , Magryś Agnieszka , Olender Alina
Published: March 2021

Purpose

Determination of the association between ica genes and phenotypic biofilm formation in staphylococcal isolates involved in conjunctivitis, their antibiotic resistance as well as detection of selected virulence characteristics: adhesion to epithelial cells and in vitro cytotoxicity.

Methods

The study included 26 Staphylococcus aureus (SA) and 26 Staphylococcus epidermidis (SE) isolates. The presence of icaAD genes and ica operon was determined by the PCR assay. Phenotypic biofilm formation was verified using the microtitre plate assay. Antibiotic resistance was performed using the disc diffusion method. Staphylococcal ability to attach to host cells was assessed by flow cytometry. Cytotoxicity on epithelial cells was evaluated by LDH assay.

Results

The ica genes were detected in 26.9% of SE and in 42.3% of SA isolates. Only 15.3% of isolates (SE) were positive for both the icaAD and the ica operon. Phenotypically, 19.2% of SE isolates were strong biofilm producers, among which three were both icaAD- and ica operon-positive. About 26.9% of SA isolates were strong biofilm producers. Methicillin resistance (MR) was detected in 34.6% of SE and 26.9% of SA isolates. About 75% of MR isolates were multidrug resistant. SA isolates adhered to host cells more extensively than SE. SA isolates released higher level of LDH than SE.

Conclusions

Adherence abilities were commonly observed in staphylococci associated with conjunctivitis. However, low prevalence of isolates positive for a complete and functional ica locus and low prevalence of strong biofilm producers was detected. SA adhered to a greater extent to eukaryotic cells than SE and were more cytotoxic.
Low-temperature sintered Ni–Zn–Co–Mn–O spinel oxide ceramics for multilayer NTC thermistors
Authors: Reimann Timmy , Töpfer Jörg
Published: March 2021

The phase formation, sintering behavior and electrical properties of Ni–Co–Zn–Mn spinel NTC thermistor ceramics of the series Ni0.5ZnzCo0.5Mn2−zO4 with 0 ≤ z ≤ 1 were studied. In contrast to NiMn2O4, which exhibits limited stability in air below 730 °C and above 970 °C, the Zn–Co-substituted nickel manganite spinels are stable at T < 730 °C and decompose at Td > 900 °C, with Td increasing with decreasing Zn Content z. The samples were sintered at 900 °C with addition of 3 wt% Bi2O3 as sintering aid and densities of above 92% were achieved. The room temperature resistivity and thermistor B-constants are almost independent of composition at 0 ≤ z ≤ 0.6 and start to increase at higher Zn concentrations. A multilayer NTC thermistor was fabricated using green tapes of a spinel of composition z = 0.75, commercial Ag paste, and cofiring at 900 °C. The firing behavior, microstructure formation and electrical properties of the multilayer thermistor are reported.
NMR refinement and peptide folding using the GROMACS software
Authors: Sinelnikova Anna , Spoel David van der
Published: March 2021

Nuclear magnetic resonance spectroscopy is used routinely for studying the three-dimensional structures and dynamics of proteins and nucleic acids. Structure determination is usually done by adding restraints based upon NMR data to a classical energy function and performing restrained molecular simulations. Here we report on the implementation of a script to extract NMR restraints from a NMR-STAR file and export it to the GROMACS software. With this package it is possible to model distance restraints, dihedral restraints and orientation restraints. The output from the script is validated by performing simulations with and without restraints, including the ab initio refinement of one peptide.
Semi-Lagrangian Subgrid Reconstruction for Advection-Dominant Multiscale Problems with Rough Data
Authors: Simon Konrad , Behrens Jörn
Published: March 2021

We introduce a new framework of numerical multiscale methods for advection-dominated problems motivated by climate sciences. Current numerical multiscale methods (MsFEM) work well on stationary elliptic problems but have difficulties when the model involves dominant lower order terms. Our idea to overcome the associated difficulties is a semi-Lagrangian based reconstruction of subgrid variability into a multiscale basis by solving many local inverse problems. Globally the method looks like a Eulerian method with multiscale stabilized basis. We show example runs in one and two dimensions and a comparison to standard methods to support our ideas and discuss possible extensions to other types of Galerkin methods, higher dimensions and nonlinear problems.
Superior outcomes with Argatroban for heparin-induced thrombocytopenia: a Bayesian network meta-analysis
Authors: Colarossi Giorgia , Maffulli Nicola , Trivellas Andromahi , Schnöring Heike , Hatam Nima , Tingart Markus , Migliorini Filippo
Published: March 2021

Background Argatroban, lepirudin, desirudin, bivalirudin, and danaparoid are commonly used to manage heparin-induced thrombocytopenia related complications. However, the most suitable drug for this condition still remains controversial. Aim of the review This Bayesian network meta-analysis study compared the most common anticoagulant drugs used in the management of heparin-induced thrombocytopenia. Method All clinical trials comparing two or more anticoagulant therapies for suspected or confirmed heparin-induced thrombocytopenia were considered for inclusion. Studies concerning the use of heparins or oral anticoagulants were not considered. Data concerning hospitalisation length, thromboembolic, major, and minor haemorrhagic events, and mortality rate were collected. The network analyses were made through the STATA routine for Bayesian hierarchical random-effects model analysis with standardised mean difference (SMD) and log odd ratio (LOR) effect measures. Results Data from a total of 4338 patients were analysed. The overall mean age was 62.31 ± 6.6 years old. Hospitalization length was considerably shorter in favour of the argatroban group (SMD: − 1.70). Argatroban evidenced the lowest rate of major (LOR: − 1.51) and minor (LOR: − 0.57) haemorrhagic events. Argatroban demonstrated the lowest rate of thromboembolic events (LOR: 0.62), and mortality rate (LOR: − 1.16). Conclusion Argatroban performed better overall for selected patients with HIT. Argatroban demonstrated the shortest hospitalization, and lowest rate of haemorrhages, thromboembolisms, and mortality compared to bivalirudin, lepirudin, desirudin, and danaparoid.
The Tyranny of ‘Teaching and Learning’
Authors: Buckley Alex
Published: March 2021

The phrase ‘teaching and learning’ has essentially replaced the word ‘teaching’ in educational discourse. The linguistic shift occurred as part of a wider movement in the 1980s and 1990s to give greater attention to learning in the educational process, and the phrase served a sloganistic function. With the learning paradigm now largely uncontroversial, the phrase—like other ex-slogans—may now be carrying implications more tied to its literal meaning. This paper suggests that the constant reference to learning in the context of teaching carries the implication that teaching is always accompanied by learning. After applying Gilbert Ryle’s distinction between task and achievement verbs, the paper argues that under certain interpretations, the idea that teaching implies learning is deeply problematic. The paper proposes that we instead use ‘teaching and studying’: to communicate the deep connections between the activities carried out by teachers and students, without supporting the unhelpful idea that the activity of teaching must always lead to the achievement of learning.
The Double Intentionality of Moral Intentional Actions: Scotus and Ockham on Interior and Exterior Acts
Authors: Schierbaum Sonja
Published: March 2021

Any account of intentional action has to deal with the problem of how such actions are individuated. Medieval accounts, however, crucially differ from contemporary ones in at least three respects: (i) for medieval authors, individuation is not a matter of description, as it is according to contemporary, ‘Anscombian’ views; rather, it is a metaphysical matter. (ii) Medieval authors discuss intentional action on the basis of faculty psychology, whereas contemporary accounts are not committed to this kind of psychology. Connected to the use of faculty psychology is (iii) the distinction between interior and exterior acts. Roughly, interior acts are mental as opposed to physical acts, whereas exterior acts are acts of physical powers, such as of moving one’s body. Of course, contemporary accounts are not committed to this distinction between two ontologically different kinds of acts. Rather, they might be committed to views consistent with physicalist approaches to the mind. The main interpretative task in this paper is to clarify how Scotus and Ockham explain moral intentional action in terms of the role and involvement of these kinds of acts respectively. I argue that Scotus’s account is close to contemporary, ‘Anscombian’ accounts, whereas Ockham’s account is incompatible with them.
Financial losses for Dutch stakeholders during the 2013 aflatoxin incident in Maize in Europe
Authors: Focker M. , van der Fels-Klerx H. J. , Oude Lansink A. G. J. M.
Published: March 2021

Early 2013, high concentrations of aflatoxin M1 were found in the bulk milk of a few dairy farms in the Netherlands. These high concentrations were caused by aflatoxin B1 contaminated maize from Eastern Europe that was processed into compound feed, which was fed to dairy cows. Since the contamination was discovered in the downstream stages of the supply chain, multiple countries and parties were involved and recalls of the feed were necessary, resulting into financial losses. The aim of this study was to estimate the direct short-term financial losses related to the 2013 aflatoxin incident for the maize traders, the feed industry, and the dairy sector in the Netherlands. First, the sequence of events of the incident was retrieved. Then, a Monte Carlo simulation model was built to combine the scarce and uncertain data to estimate the direct financial losses for each stakeholder. The estimated total direct financial losses of this incident were estimated to be between 12 and 25 million euros. The largest share, about 60%, of the total losses was endured by the maize traders. About 39% of the total losses were for the feed industry, and less than 1% of the total losses were for the dairy sector. The financial losses estimated in this study should be interpreted cautiously due to limitations associated with the quality of the data used. Furthermore, this incident led to indirect long-term financial effects, identified but not estimated in this study.
Coulomb stress analysis for several filling and operational scenarios at the Grand Ethiopian Renaissance Dam impoundment
Authors: Madson Austin , Sheng Yongwei
Published: March 2021

Increased demand for power generation coupled with changing seasonal water uncertainty has caused a worldwide increase in the construction of large hydrologic engineering structures. That said, the soon-to-be-completed Grand Ethiopian Renaissance Dam (GERD) will impound the Blue Nile River in Western Ethiopia and its reservoir will encompass ~ 1763 km2 and store ~ 67 Gt (km3) of surface water. The impoundment will undergo maximum seasonal load changes of ~ 28 to ~ 36 Gt during projected seasonal hydroelectric operations. The GERD impoundment will cause significant subsurficial stresses, and could possibly trigger seismicity in the region. This study examines Coulomb stress and hydrologic load centroid movements for several GERD impoundment and operational scenarios. The maximum subsurficial Coulomb stress applied on optimally oriented fault planes from the full impoundment is ~ 186 kPa and over 30% of our model domain incurs Coulomb stresses ≥ 10 kPa, regardless of the impoundment period length. The main driver behind Coulomb stress and load centroid motion during impoundment is the annual, accumulated daily reservoir storage change. The maximum Coulomb stresses from the highest amplitude season of five long-term operational scenarios are around 36, 33, 29, 41, and 24% of the total maximum stresses from the entire GERD impoundment. Variations in annual Coulomb stresses during modeled GERD operations are attributed to the seasonal load per unit area, and partially to the initial seasonal water level. The spatial patterns and amplitudes of these stress tensors are closely linked to both the size and timing of GERD inflow/outflow rates, and an improved understanding of the magnitude and extent of these stresses provides useful information to water managers to better understand potential reservoir triggered seismic events from several different operational and impoundment strategies.
Simple Baseline Machine Learning Text Classifiers for Small Datasets
Authors: Riekert Martin , Riekert Matthias , Klein Achim
Published: March 2021

Text classification is important to better understand online media. A major problem for creating accurate text classifiers using machine learning is small training sets due to the cost of annotating them. On this basis, we investigated how SVM and NBSVM text classifiers should be designed to achieve high accuracy and how the training sets should be sized to efficiently use annotation labor. We used a four-way repeated-measures full-factorial design of 32 design factor combinations. For each design factor combination 22 training set sizes were examined. These training sets were subsets of seven public text datasets. We study the statistical variance of accuracy estimates by randomly drawing new training sets, resulting in accuracy estimates for 98,560 different experimental runs. Our major contribution is a set of empirically evaluated guidelines for creating online media text classifiers using small training sets. We recommend uni- and bi-gram features as text representation, btc term weighting and a linear-kernel NBSVM. Our results suggest that high classification accuracy can be achieved using a manually annotated dataset of only 300 examples.
Lubrication Practices and Receptive Anal Sex: Implications for STI Transmission and Prevention
Authors: Lee Austin , Gaither Thomas W. , Langston Marvin E. , Cohen Stephanie E. , Breyer Benjamin N.
Published: March 2021

Introduction

Implications of lubricant use in men having sex with men (MSM) are poorly characterized, particularly associations with sexual behavior and rectal sexually transmitted infection (STI) risk.

Aim

We sought to clarify covariates associated with lubrication type including differing sexual preferences and rectal STI prevalence.

Methods

Primary English-speaking individuals ≥18 years old visiting San Francisco City Clinic (SFCC) between April and May of 2018 who endorsed lubricant use during receptive anal sex within the last 3 months were studied. Associations between lubrication type used and collected covariates were assessed using Kruskal-Wallis analysis of variance for continuous variables and Chi-squared test for categorical variables. We used logistic regression to examine the association between lubrication type and rectal STI test result.

Main Outcome Measures

Rectal STI test positivity.

Results

From all enrolled participants, 179 completed the survey and endorsed use of a lubricant during receptive anal sex within the last 3 months. Silicone lubricant users had the most sexual partners in the last 3 months (13 [mean] ± 30 [SD], P= .0003) and were most likely to have a history of gonorrhea. Oil-based lubricant users had the most partners with whom they had receptive anal sex in the last 3 months (7 ± 6, P= .03). Water-based lubricant users most commonly used a condom in their last sexual encounter and had the fewest sexual partners in the last 3 months (4 ± 4, P= .0003). Spit/saliva lubricant use was associated with positive rectal STI result.

Conclusion

Silicone and oil-based lubricant users were more likely to report condomless receptive anal sex and to have a history of gonorrhea while spit/saliva lubricant use associated with positive rectal STI acquisition.
La Charte québécoise des droits et libertés permet-elle de mobiliser l’intersectionnalité comme cadre d’analyse de la discrimination? Quelques pistes de réflexion
Authors: Tanguay Vanessa
Published: March 2021

Cet article s’interroge sur les différentes possibilités susceptibles d’éclairer la norme juridique antidiscriminatoire québécoise, soit l’interdiction de discrimination prévue à l’article 10 de la Charte des droits et libertés de la personne du Québec, par le cadre théorique de l’intersectionnalité. On procédera à un bref survol de la pensée intersectionnelle telle que développée dans les disciplines externes au droit ainsi qu’un portrait de son intégration au droit sont dressés afin de comprendre la pertinence de l’intersectionnalité pour appréhender la « réalité » sociale de la discrimination. Quelques points d’ancrage de la mobilisation de l’intersectionnalité seront présentés dans une perspective de réinvestissement du droit vers une appréhension élargie de cette « réalité » sociale par la norme juridique. Il est ainsi proposé de repenser les catégorisations juridiques et de déconstruire les dynamiques d’oppression en ouvrant des possibilités de mobilisation concrètes au moment de l’énonciation et de l’interprétation par la pratique judiciaire des éléments constitutifs de l’acte discriminatoire.
38189 Potential effect of serum from hypertensive donors on PP2A expression and activity in endothelial cells
Authors: Gomez Dulce H. , Aldokhayyil Maitha , Robinson Austin T. , Brown Michael D.
Published: March 2021

ABSTRACT IMPACT: Racial differences in the prevalence of hypertension and endothelial (dys)function are well established, yet research investigating the mechanism(s) underlying this disparity is still lacking. OBJECTIVES/GOALS: Investigate the influence of race and the effect of serum collected from hypertensive donors on Protein Phosphatase 2A (PP2A) and endothelial nitric oxide synthase (eNOS) expression and activity in human umbilical vein endothelial cells (HUVECs) from Caucasian (CA) and African American (AA) donors. METHODS/STUDY POPULATION: HUVECs from 3 CA & 3 AA donors were cultured in parallel. Experiments were conducted between passages 5-7. At ?90% confluency, cells were serum starved ˜12hrs prior to incubating for 24 or 48 hours in one of the following conditions: 1) Control (Fetal Bovine Serum), 2) serum from normotensives (NT; 5 CA & 5 AA donors), or 3) serum from hypertensives (HT; 5 CA & 5 AA donors). NT and HT serum was pooled from donors with the following characteristics: Male, 30-50 years, nonsmokers, no comorbidities, and non-obese (BMI < 30 kg/m2). Western blotting was used to measure protein expression of total eNOS, p-eNOSS1177, total PP2A, and p-PP2AY307. For activity p-eNOSS1177/total eNOS and p-PP2AY307/ total PP2A ratio was used. A two-way ANOVA was used for statistical analysis. RESULTS/ANTICIPATED RESULTS: Irrespective of the donors’ race, there was no influence of serum treatment or interaction effect in any of the measured proteins of interest. Moreover, compared to CA, HUVECs from AA had lower expression of eNOS irrespective of condition (race p=0.01). Compared to CA, HUVECs from AA tended to have lower expression of p-eNOSS1177 irrespective of condition (race p=0.07). However, there was no racial differences in eNOS activity (p=0.68). There was no racial difference in the expression of PP2A (p=0.35), p-PP2AY307 (p=0.30), or PP2A activity (p=0.97) in all conditions. DISCUSSION/SIGNIFICANCE OF FINDINGS: Our preliminary results suggest no influence serum constituents from hypertensive donors or race on PP2A or eNOS expression and activity in HUVECS. Future research should consider conducting proteomics profiling to compare NT and HT serum.
99529 The Role of ATF6-Mediated Signaling in PARP Inhibitor Resistant Ovarian Cancer
Authors: Alexandra  , Bitler Benjamin
Published: March 2021

ABSTRACT IMPACT: This work has the potential to identify targetable pathways conveying resistance to PARP inhibitors that may improve ovarian cancer patient outcomes. OBJECTIVES/GOALS: High grade serous ovarian cancer is the deadliest gynecologic malignancy. PARP inhibitors are an FDA approved targeted therapy that is being used more and more frequently in the clinic. It is vital to understand mechanisms driving resistance to this therapy in order to develop treatments to improve patient responses. METHODS/STUDY POPULATION: RNA-sequencing and transcription factor analysis was used to identify pathways of interest. An AP-1 transcriptional reporter assays was used to confirm results of the transcription factor analysis. An unbiased lentiviral shRNA screen was used to identify AP-1 subunits promoting PARP inhibitor resistance. Lentiviral transduction allowed for the knockdown ATF6. Comet assays and two-plasmid systems were used to determine levels of DNA damage and levels of DNA damage repair respectively. RESULTS/ANTICIPATED RESULTS: PARP inhibitor resistant cell lines have increased WNT signaling which promotes to increased DNA damage repair. PARP inhibitor resistant cell lines also have increased AP-1 transcriptional activity, ATF6 expression, and active p38. ATF6 knockdown and p38 inhibition is sufficient to resensitize cells to PARP inhibition. Upon treatment with PARP inhibitors, ATF6 knockdown as well as p38 inhibition lead to increased DNA damage in PARP inhibitor resistant cell lines. RNA-sequencing reveals a signifcant overlap in downregulated genes in cells treated with a β-catenin inhibitor and cells with an ATF6 knockdown. DISCUSSION/SIGNIFICANCE OF FINDINGS: Due to the increasing prevalence of PARP inhibitors in the clinic, it is vital to uncover mechanisms contributing to resistance. This work has the potential to identify targetable pathways conveying resistance to PARP inhibitors that may improve ovarian cancer patient outcomes.
39347 Examining the Potential for Tech-Based HIV interventions for Young Black Women
Authors: Craddock Jaih B.
Published: March 2021

ABSTRACT IMPACT: Findings from this study have the potential to improve interventions geared toward YBW, by highlighting the potential for technology-based HIV interventions. OBJECTIVES/GOALS: A stark disparity in HIV exists for Black American women, with 61% of all new HIV infections among American women occurring in Black women. Using technology to address community-level HIV risk may be beneficial, however few studies have examined the association of tech-based communication and HIV prevention behaviors among Black women. METHODS/STUDY POPULATION: Egocentric social network data from 201 young Black women (YBW) were collected from June 2018 to December 2018 to identify how social media use (e.g., amount of time, type of social media used, health information seeking) and sexual health communication (e.g., talk about condom use via face-to-face, text or phone and talk about HIV testing via face-to-face, text or phone) were associated with condom use, HIV testing and interest in pre-exposure prophylactic (PrEP). Statistical analysis proceeded in two stages, descriptive statistics and multivariate logistic regression modeling. RESULTS/ANTICIPATED RESULTS: Instagram (82%) and Snapchat (79%) were the most used social media platforms for communication with SNMs. About 20% of YBW reported spent 4 or more hours on social media per day, and a majority of YBW spoke to at least one SNM via text (85%), face-to-face (98%), or on the phone (97%). Multivariate logistic regression results indicated that YBW who spoke to their SNMs via Instagram had 3.23 times the odds of using condom during last sex, however if they spoke to SNMs on twitter or spent more then 4 hours on social media they had a decrease in odds of using condoms. YBW had 96% decreased odds of ever being tested for HIV if they spoke to a SNM face-to-face about condom use; and notably, YBW had 3 times the increased odds in interest of using PrEP if they had sex with someone they met online or if they sought sexual health information online. DISCUSSION/SIGNIFICANCE OF FINDINGS: By assessing the modes of communication YBW are using to speak with their SNMs and their associations with HIV prevention behaviors, we can better determine the most optimal, efficient, and effective ways of utilizing technology for HIV intervention.
69399 How are substance use disorder treatment programs in Arkansas responding to COVID-19? A qualitative study
Authors: Baloh Jure , Curran Geoffrey M.
Published: March 2021

ABSTRACT IMPACT: This study informs how substance use treatment programs responded to the COVID-19 pandemic, and highlights implication for future translational research and practice. OBJECTIVES/GOALS: The COVID-19 pandemic rapidly changed how substance use disorder (SUD) treatment services are organized and provided. This study examined what changes SUD treatment programs in Arkansas implemented (e.g., guidelines, technologies), and what factors influenced their ability to implement and sustain these changes. METHODS/STUDY POPULATION: Between May and August 2020, we conducted semi-structured phone interviews with 29 leaders (administrative and/or clinical leaders) at 21 residential and outpatient SUD treatment programs throughout Arkansas (i.e., in all five Arkansas public health regions). Interviews were based on the Consolidated Framework for Implementation Research and focused on what changes programs were implementing in response to the COVID-19 pandemic, barriers and facilitators to implementation, and recommendations for future. The interviews were on average about 30 minutes long, and we provided no participant compensation. Interviews were recorded and transcribed verbatim, then thematically analyzed. RESULTS/ANTICIPATED RESULTS: Programs implemented similar infection control practices: screening at entry, masks, hand hygiene, and social distancing. Residential programs stopped outside visitations and some capped admissions; outpatient programs stopped group sessions and switched most services to telehealth. Key facilitators included grants/loans (e.g., salaries), looser regulatory restrictions (e.g., telehealth), and good coordination with other organizations (e.g., state agencies). Key barriers included limited access to supplies (e.g., masks), no rapid testing (particularly for residential care), limited capacity for social distancing, and negative employee and client responses (e.g., anxiety). Key recommendations include better access to supplies and testing, telehealth continuation and better communication. DISCUSSION/SIGNIFICANCE OF FINDINGS: This study provides an insight into how SUD programs responded to the COVID-19 pandemic and what the ‘new normal’ is. This can inform D&I studies conducted in SUD settings, including studies examining what implementation strategies can help sustain these changes, or studies of other practices implemented during or after the pandemic.
58201 What does team science look like across the CTSA Consortium? A qualitative analysis of the Great CTSA Team Science Contest results
Authors: Pelfrey Clara , Goldman Ann , DiazGranados Deborah
Published: March 2021

ABSTRACT IMPACT: This paper reveals the myriad techniques that CTSA hubs use to support, promote and expand team science including many ways to involve the community, students, scholars and other multidisciplinary scientists. OBJECTIVES/GOALS: The Great CTSA Team Science Contest (GTSC) was developed in the NCATS Workgroup on Institutional Readiness for Team Science to collect stories describing the many ways hubs were promoting and supporting team science across the CTSA consortium. METHODS/STUDY POPULATION: Our qualitative data analysis examined the different designs from a high level - namely we categorized how many of the stories were competitions for pilot funding, training programs on team science competencies, communication skills training, workshops for educating community collaborators about research and/or training investigators about community-based research, advancing promotion and tenure for team science, etc. We discuss specific examples of different designs and who they were intended to benefit. RESULTS/ANTICIPATED RESULTS: Launched in July 2018, the contest received 170 submissions from 45 unique CTSA hubs. Qualitative analysis addressed the following questions about team science: 1) Who or what group championed it? 2) Who benefitted or who were the intended recipients? 3) What was the desired outcome? (e.g. team science skills, communication skills, getting the community involved, fostering new collaborations, expanding capacity for team science, etc.) 4) What method(s) did they use? 5) What translational science stage was addressed? DISCUSSION/SIGNIFICANCE OF FINDINGS: This analysis includes examples of team science research, resources or interventions including successful team dynamics and knowledge integration. This paper reveals the myriad techniques that CTSA hubs use to support, promote and expand team science including involving the community, students, scholars and other multidisciplinary scientists.
48390 Racial and Ethnic Disparities in Pediatric Kidney Transplantation - Has KAS made a difference?
Authors: Charnaya Olga , Yu Sile , Goldberg Aviva , Garonzik-Wang Jacqueline , Segev Dorry , Verghese Priya S.
Published: March 2021

ABSTRACT IMPACT: Evaluate the impact that the Kidney Allocation System has had on racial and ethnic disparities in pediatric deceased donor kidney transplant recipients. OBJECTIVES/GOALS: Racial and ethnic minority pediatric transplant candidates have known disparities in access to kidney transplantation. The Kidney Allocation System (KAS), implemented in 2014, was designed in part to alleviate some of these disparities thereby making transplant more equitable. We investigated the effect of KAS on reported disparities. METHODS/STUDY POPULATION: We utilized Scientific Registry of Transplant Recipients (SRTR) data to determine differences in new waitlist registrants, deceased donor (DDKT) and living donor kidney transplants (LDKT), HLA mismatch, and allograft survival among pediatric patients of different racial and ethnic backgrounds. RESULTS/ANTICIPATED RESULTS: Black pediatric patients represented 21.3% of new waitlist registrants pre-KAS and 18.9% post-KAS. Waitlist time increased for pediatric patients of all races post-KAS with the highest increase (131 days) in Asian patients (p < 0.01). The racial distribution of DDKT pre- and post-KAS was unchanged (White 38.4% vs 38.3%, Black 24.5% vs 22.5%, Hispanic 30.6% vs 31.1%, Asian 3.7% vs 4.4%, p = 0.12). The 3-yr graft failure rate is disproportionately worse in Black children compared to other races pre- and post-KAS (White 6.8% vs 5.3%, Black 14% vs 8.7%, Hispanic 8% vs 4.5%, Asian 6.6% vs 6.7%, Other 6.5% vs 2.9%) although there is a trend towards better graft survival in the post-KAS era. Graft survival worsened in Asian children in the post-KAS era (HR 2.34,95% CI 1.05 - 5.25, p=0.038). DISCUSSION/SIGNIFICANCE OF FINDINGS: Racial and ethnic disparities in pediatric ESRD patients have not been ameliorated by KAS. Children of color have longer waitlist time and are more likely to have graft failure. Alarmingly, allograft failure rate increased in Asian patients post-KAS, which merits further evaluation.
54443 Pharmacogenomic Profiling of East and West African Populations
Authors: Jackson Linsey , Guerrero Ruben Bonilla , Athreya Arjun , Roberts Lewis R.
Published: March 2021

ABSTRACT IMPACT: Genomic variation likely plays a role in differences in rates of adverse reactions and efficacy for African populations, and this research will add to the understudied field of pharmacogenomics in African populations. OBJECTIVES/GOALS: We aim to characterize the frequency of variants in clinically relevant genes in East and West African populations to assess the prevalence of potential drug-gene interactions. METHODS/STUDY POPULATION: Our pilot study will consist of 100 Somali patients enrolled at Mayo Clinic Rochester and 100 Ghanaian patients recruited at Teaching Hospitals in Ghana. Germline DNA will be extracted from pre-existing blood samples. Sequencing will be performed using Admera Health’s PGxOne Plus test, interrogating a panel of 62 genes. Variants will be reported along with the predicted response for a list of drugs. Differences between frequencies of variants in East and West African populations will be analyzed. We will look for correlations with reported adverse reaction rates. We will then compare our findings with allele frequency data from publicly available data bases. Additionally, we will analyze the flanking regions of the panel for novel variants, using machine learning to predict gene-drug interactions. RESULTS/ANTICIPATED RESULTS: African populations are known to have more genetic diversity than any other population. Additionally, only African-Americans, African-Caribbeans from Barbados, Esan and Yoruba Nigerians, Gambians, Kenyans, and Sierra Leoneans are accounted for within the publicly available data bases most often used for variant studies. Thus, it is anticipated that we will find differences in the variant allele frequencies of our populations compared to European allele frequencies, and differences in frequencies between the East and West African populations. In the 200 base pair flanking regions that are sequenced in the assay along with the known variant regions, we may find novel previously unreported variants. DISCUSSION/SIGNIFICANCE OF FINDINGS: The lack of knowledge of pharmacogenomic variation in African populations contributes to ethnic disparities in patient outcomes. This study addresses this gap by adding to our comprehension of variants in clinically relevant genes, giving insight into underlying mechanisms of ethnicity-based drug responses.
15000 Exploring team science, professional networks, and innovation success in the THRIVE COVID-19 fellowship program
Authors: Fattah Layla , Gabrilove Janice , Oemke Holly , Borrello Joseph , Baker Turner , Costa Kevin D. , Putrino David , Costa Anthony
Published: March 2021

ABSTRACT IMPACT: Implement and evaluate a fellowship program to foster a new generation of entrepreneurial and collaboratively-minded team scientists, equipped with the knowledge and skills to innovate technology-based solutions for COVID-19 to advance human health OBJECTIVES/GOALS: Mount Sinai Targeted Healthcare Innovation Fellowship (THRIVE) is a 9-month program for participants from diverse professional backgrounds to develop HealthTech innovations related to COVID-19. The program is designed to provide an experiential team science platform for fellows to take an idea from concept to commercially viable innovation. METHODS/STUDY POPULATION: Following a competitive application process, 16 THRIVE fellows comprise four teams working collaboratively in an online forum with input from experts in the field. Success of the program will be evaluated by:

assessing pre- and post- collaborative research orientation among THRIVE fellows using the ROI scale1

using social network analysis (SNA) to investigate the social networks of THRIVE fellows to capture patterns of communication and collaboration related to innovation development

exploring participant experiences of group formation, teamwork and collaboration related to innovation development using one-to-one semi-structured interview

determining team success in innovation development, measured by number of publications, funding awarded, provisional patents and viable products. RESULTS/ANTICIPATED RESULTS: Paired t-tests will determine whether collaborative orientation of THRIVE fellows changes pre- vs. post- program participation, indicating changes in attitude toward multidisciplinary team work. SNA will be used to describe structural patterns of communication that occur at individual and group levels. Network-level indices will provide insight into patterns of communication that exist in innovation development: degree centrality (number of connections per individual), betweenness centrality (number of bridges to others in a network), closeness centrality (closeness to others in a network). We will also test for associations between network characteristics and team success. DISCUSSION/SIGNIFICANCE OF FINDINGS: Understanding patterns of formal and informal relationships, interactions, and perceptions of the collaborative process among individuals in THRIVE teams will elucidate whether such a program can provide an effective forum for team science and innovation development related to COVID-19.
66361 TL1 Team Approach to Predicting Response to Spinal Cord Stimulation for Chronic Low Back Pain
Authors: See Kyle , Ho Rachel , Coombes Stephen , Fang Ruogu
Published: March 2021

ABSTRACT IMPACT: Understanding how spinal cord stimulation works and who it works best for will improve clinical trial efficacy and prevent unnecessary surgeries. OBJECTIVES/GOALS: Spinal cord stimulation (SCS) is an intervention for chronic low back pain where standard interventions fail to provide relief. However, estimates suggest only 58% of patients achieve at least 50% reduction in their pain. There is no non-invasive method for predicting relief provided by SCS. We hypothesize neural activity in the brain can fill this gap. METHODS/STUDY POPULATION: We tested SCS patients at 3 times points: baseline (pre-surgery), at day 7 during the trial period (post-trial), and 6 months after a permanent system had been implanted. At each time point participants completed 10 minutes of eyes closed, resting electroencephalography (EEG) and self-reported their pain. EEG was collected with the ActiveTwo system and a 128-electrode cap. Patients were grouped based on the percentage change of their pain from baseline to the final visit using a median split (super responders > average responders). Spectral density powerbands were extracted from resting EEG to use as input features for machine learning analyses. We used support vector machines to predict response to SCS. RESULTS/ANTICIPATED RESULTS: Baseline and post-trial EEG data predicted SCS response at 6-months with 95.56% and 100% accuracy, respectively. The gamma band had the highest performance in differentiating responders. Post-trial EEG data best differentiated the groups with feature weighted dipoles being more highly localized in sensorimotor cortex. DISCUSSION/SIGNIFICANCE OF FINDINGS: Understanding how SCS works and who it works best for is the long-term objective of our collaborative research program. These data provide an important first step towards this goal.
78046 The Kansas City Quality & Value Innovation Consortium (KC QVIC): Leveraging Team Science, Translational Research and Training to Improve the Value of Healthcare in the Community
Authors: Farr Stacy , Spertus Jon , Stowe James , Hagle Holly , Sawyer Jacque
Published: March 2021

ABSTRACT IMPACT: Implementing a team science approach with broad engagement from academic researchers, healthcare payers, providers, patients, and community-based organizations is complex, yet critical to implementing evidence into real world settings. OBJECTIVES/GOALS: 1. Participants will be able to deploy novel strategies for creating and training a regional multi-stakeholder consortium to improve the quality and value of healthcare.

2. Participants will be to examine ways in which team science provides holistic sustainable strategies to improve care and outcomes in real-world settings. METHODS/STUDY POPULATION: The Quality & Value Innovation Consortium (QVIC) has created a network of hospitals and other stakeholders (providers, payers, purchasers, patients, community-based organizations, and researchers) to collaborate and innovate on healthcare delivery. This initiative began with a team of a physician researcher, a health services researcher, and a nurse researcher first identifying healthcare systems’ priorities through individual meetings with leadership from 14 regional hospitals. Concurrently, meetings were held with other stakeholders. These interviews identified 32 key quality improvement topics.

Focus groups and surveys reduced these to 11 topics that were then selected for community forums. Through a mixed methods approach, two priority topics were selected for regional implementation. RESULTS/ANTICIPATED RESULTS: The QVIC meetings have prioritized two topics and highlighted novel information sharing across entities, and strategies to address the social determinants of health. The QVIC efforts have been recognized as a community asset for helping build collaboration and partnerships among diverse stakeholders. Ultimately, two regional initiatives, opioid management, and transitions in heart failure care were selected for implementation. Both of these initiatives aim to reduce readmissions by addressing social determinants of health. Implementation strategies and evaluation metrics are being customized for pragmatic integration within each system, utilizing a collaborative team science approach. DISCUSSION/SIGNIFICANCE OF FINDINGS: While the entire country is grappling with the challenge of improving the quality of care, while lowering its costs, Kansas City has modeled a unique culture and strategy for achieving this goal, important for learning health systems and communities.
66534 Evaluation plans for a summer child nutrition assistance program to better understand translation of policy to community health
Authors: Lee Jiwoo , Fulkerson Jayne A. , Harnack Lisa J. , Guan Weihua
Published: March 2021

ABSTRACT IMPACT: Study findings can guide improvements of the Summer Food Service Program to maximize the program’s desired effects on child summer nutrition and related health outcomes. OBJECTIVES/GOALS: The Summer Food Service Program (SFSP) addresses food insecurity during summer months. Project specific aims are to: 1. Describe characteristics of children participating in the SFSP. 2. Determine the nutritional quality of the SFSP foods. 3. Evaluate changes in children’s food insecurity, diet quality, and body mass index by SFSP participation. METHODS/STUDY POPULATION: A single group, prospective, staggered cohort design will be used for the proposed study. Two cohorts of 30 (N=60) elementary students and their parents will be recruited during the 2021-22 and 2022-23 school year. Each participant will complete a measurement session at three time-points: Baseline (spring), Post-Program (program end), and Follow-Up (following spring). Parents will complete an online survey about household food insecurity and family socio-demographic characteristics. Children will complete three 24-hour dietary recall interviews, and their heights, weight and percent body fat will be measured. The menus of at least ten SFSP sites will be analyzed to determine the nutritional adequacy of the site menus by using the Healthy Eating Index-2015. RESULTS/ANTICIPATED RESULTS: Study hypotheses are as followed: Aim 1. Not all of the children participating in the SFSP are from food-insecure or low-income households. Aim 2. Meals served at the SFSP will be higher in sugar and fat and lower in fruits and vegetables compared to recommendations in the 2015-2020 Dietary Guidelines for Americans. Additionally, the Healthy Eating Index-2015 score of the SFSP menus will be lower than that of the National School Lunch Program menus. Aim 3. Consistent SFSP participation will have a positive effect on reducing food insecurity, but not on increasing diet quality and reducing body mass index and percent body fat in children. DISCUSSION/SIGNIFICANCE OF FINDINGS: Program user information will determine if the program is reaching the target audience. Program managers will utilize menu analysis results to improve their menu nutritional quality. Changes in food insecurity, diet quality and anthropometric measures will inform whether the program needs to be improved to prevent any untoward excess weight gain.
Asymptotic expansion of low-energy excitations for weakly interacting bosons
Authors: Boßmann Lea , Petrat Sören , Seiringer Robert
Published: March 2021

We consider a system ofNbosons in the mean-field scaling regime for a class of interactions including the repulsive Coulomb potential. We derive an asymptotic expansion of the low-energy eigenstates and the corresponding energies, which provides corrections to Bogoliubov theory to any order in$1/N$.