bluuebunny/crossref_metadata_2025 huggingface dataset:
Assessment of Five Pesticides as Endocrine-Disrupting Chemicals: Effects on Estrogen Receptors and Aromatase
Authors: Gea Marta , Zhang Chao , Tota Roberta , Gilardi Gianfranco , Di Nardo Giovanna , Schilirò Tiziana
Published: February 2022

Pesticides are widely applied all over the world, and pesticide exposure can induce different biological effects posing a possible threat to human health. Due to their effects on the endocrine system, some pesticides are classified as endocrine disruptors. The aim of the study is to assess the interference of five pesticides on estrogen biosynthesis and estrogen signaling. Three neonicotinoid insecticides (Acetamiprid, Clothianidin, and Thiamethoxam), a carbamate insecticide (Methiocarb) and a herbicide (Oxadiazon) were tested. The effect of pesticides on estrogen biosynthesis was studied through an ELISA assay using a recombinant form of human aromatase, the enzyme that catalyzes the transformation of androgens to estrogens. Moreover, the effect of pesticides on estrogen signaling was assessed using a gene reporter assay on MELN cells, which measures estrogen receptor-mediated estrogenic activity. The results of the ELISA assay showed that the pesticides did not alter aromatase activity (no interference with estrogen biosynthesis), while the results of the gene reporter assay showed that only Methiocarb was able to alter estrogen signaling at high doses. The estrogenic activity of Methiocarb, expressed as 17β-estradiol equivalency factor (EEF), was equal to 8.0 × 10−8. In conclusion, this study suggested that Methiocarb should be considered a potential endocrine disruptor.
College Student E-Cigarette Users’ Knowledge about E-Cigarettes: Ingredients, Health Risks, Device Modifications, and Information Sources
Authors: McLeish Alison C. , Hart Joy L. , Walker Kandi L.
Published: February 2022

Although college students represent a growing demographic of e-cigarette users, it is unclear how knowledgeable they are about the product they use. The lack of such knowledge could result in unsafe practices and greater health risks. Therefore, the purpose of the current study was to examine college student e-cigarette users’ knowledge about e-cigarette ingredients and health risks, how often they modify their devices, and whether they utilize reputable sources when searching for information regarding e-cigarettes. The participants were 183 undergraduate e-cigarette users (Mage = 19.98, SD = 1.98; 71.6% female; 85.8% White). Most participants correctly recognized that e-cigarettes increase the risk of cardiovascular disease and lung disease, but fewer than half recognized the increased risk of seizures and depression. Only one-third to one-half of participants correctly identified the toxic compounds commonly found in e-cigarettes, and most indicated that they would consult Google or a friend with questions about e-cigarettes. College student e-cigarette users are well-informed about many health risks associated with e-cigarettes. However, they are relatively unaware of the harmful substances in e-cigarettes and are seeking information from less reliable sources. Targeted public health campaigns educating college students about e-cigarettes, including where to seek reliable information, are needed.
Retention Ability of a Glass Carbomer Pit and Fissure Sealant
Authors: Beresescu Liana , Kovacs Monika , Vlasa Alexandru , Stoica Alexandra Mihaela , Benedek Csilla , Pop Mihai , Bungardean Denisa , Eșian Daniela
Published: February 2022

Dental sealants are an excellent means to prevent pits and fissure decay. Currently, there are multiple commercially available sealant materials. The purpose of this study was to assess the retention of glass carbomer fissure sealant and the incidence of secondary caries over a period of 24 months in comparison with a resin-based sealant. Materials and Methods: We included 32 children in the study, with ages between six and eight years and an average age of 6.8 years old. For each child, we sealed four permanent molars (totaling 128 teeth). The study group was divided into sub-groups. Sub-group A was represented by 64 first permanent molars which underwent dental sealing procedures with composite resin-based fissure sealant, Helioseal F™, and sub-group B was represented by 64 first permanent molars which underwent dental sealing procedures with glass carbomer cement, GCP Glass Seal™. The sealants were assessed clinically at 6, 12, 18, and 24 months. Results: The 6-month follow-up evaluation showed no statistically significant differences between the two materials neither regarding sealant retention nor new carious lesions formation (p > 0.05). At the 12-month recall, 57 molars had good retention (89.06%) from sub-group A and 44 molars (68.75%) from sub-group B; there was a statistically significant difference (p = 0.0187) between the two treatment choices only regarding material retention. At the last recall after 2 years, sub-group A had a higher number of molars with perfect sealing (47–73.43%) and 8 molars (12.5%) with new caries lesions and sub-group B had 23 (35.93%) molars with perfect sealing and 15 molars (23.44%) with new caries lesions; there was a statistically significant difference (p < 0.0001) between the two treatment choices only regarding material retention. Conclusions: The glass carbomer retention is very inferior to the resin-based material. The glass carbomer sealant was effective in preventing new caries development, comparable with the conventional resin-based sealant.
Designing a Patient-Friendly Website for Newly Diagnosed Cancer Patients with the Participatory Health Research Approach
Authors: Rackerseder Juliane , Hornbach Carolin , Dicks Peter , Kerek-Bodden Hedy , Krieger Theresia
Published: February 2022

High-quality and user-friendly patient information material (PIM) is essential for understanding and accepting a new care programme. When optimising the PIM of the integrated, cross-sectoral psycho-oncological (isPO) care programme, the design of the fifth element of the patient information strategy—the patient-friendly website—was still pending. In this paper, the iterative design process of the patient-friendly isPO website is described. We applied the participatory health research (PHR) approach to enable high levels of participation of its respective end-users (e.g., cancer survivors), service providers, and experts. The design included six steps: (1) initiation, (2) planning, (3) initial idea exploration, (4) creation of a first working version, (5) three optimisation loops, and (6) dissemination. An exploratory mixed-methods design has been used. Qualitative data collection included document analysis, interviews, and participatory action research (PAR) loops with focus groups. Finally, the quality of the newly designed website was quantitatively assessed with the UPIM-Check, a user-friendly instrument for assessing and optimising PIM. The PHR approach was indispensable for the design of our needs-oriented, patient-friendly website. Participants’ high levels of participation strongly contributed to the products’ quality. The final descriptive statistical evaluation shows that the final website was rated very good on average by its end-users.
Perceived Partner’s Self-Control and Social Support Effects on Relationship Satisfaction in Couples Experiencing Infertility or Miscarriage: Dyadic Analyses
Authors: Wendołowska Anna , Kiełek-Rataj Ewa , Kalus Alicja , Czyżowska Dorota
Published: February 2022

The process that infertile couples and those after a miscarriage go through is unpredictable and difficult to control; therefore, it is associated with a lowered sense of control for both partners. Uncontrolled stress creates a higher level of anxiety, which is associated not only with a lower quality of life but also with worse results from infertility treatment and higher risks of miscarriage. The aim of this study was to analyze the relationship between the partner’s perceived self-control and marital satisfaction in the context of the partners’ coping strategies. The actor-partner interdependence model was applied to 90 heterosexual married couples. Our results show that men who perceive their wives as being more self-controlled and women who are perceived by their husbands as being more self-controlled feel more satisfied in their relationships. The effect of a partner’s perceived self-control on satisfaction with the relationship was weaker when controlled for the length of marriage. It also appeared to be moderated through the spouses’ use of social support. We conclude that the effects of the partner’s perceived self-control and social support are strong for marital satisfaction in the context of infertility and miscarriage.
Survival Rates of Endodontically Treated Posterior Teeth Restored with All-Ceramic Partial-Coverage Crowns: When Systematic Review Fails
Authors: Ferrari Marco , Ferrari Cagidiaco Edoardo , Pontoriero Denise Irene Karin , Ercoli Carlo , Chochlidakis Kostantinos
Published: February 2022

Background: To determine the survival rates of endodontically treated posterior teeth (EDPT) restored with partial coverage all-ceramic crowns with or without the use of fiber posts. Methods: MEDLINE and Cochrane searches were conducted in order to identify Randomized Clinical Trials (RCTs) related to endodontically treated posterior teeth restored with partial coverage crowns. The search period was extended until February 2020 and only in vivo, human, and studies in the English language were included. A manual search was also conducted and additional articles, if found, were included in the database. Results: The initial search for the selected databases identified 495 studies, which were all screened for inclusion through titles, abstracts and full-text reading. Out of these 495 studies, only one article met the eligibility criteria and was included in this systematic review. Statistical analysis could not be performed. Conclusions: Only one RCT was identified in this systematic review. More clinical evidence is necessary to assess the survival rate of EDPT with partial-coverage crowns. This systematic review failed because it did not find scientific evidence to support the use of indirect bonded restorations on EDPT.
Experiences of Perinatal Mental Health Care among Minority Ethnic Women during the COVID-19 Pandemic in London: A Qualitative Study
Authors: Pilav Sabrina , Easter Abigail , Silverio Sergio A. , De Backer Kaat , Sundaresh Sushma , Roberts Sara , Howard Louise M.
Published: February 2022

(1) Background: Approximately one in five women will experience mental health difficulties in the perinatal period. Women from ethnic minority backgrounds face a variety of barriers that can prevent or delay access to appropriate perinatal mental health care. COVID-19 pandemic restrictions created additional obstacles for this group of women. This study aims to explore minority ethnic women’s experiences of perinatal mental health services during COVID-19 in London. (2) Methods: Eighteen women from ethnic minority backgrounds were interviewed, and data were subject to a thematic analysis. (3) Results: Three main themes were identified, each with two subthemes: ‘Difficulties and Disruptions to Access’ (Access to Appointments; Pandemic Restrictions and Disruption), ‘Experiences of Remote Delivery’ (Preference for Face-to-Face Contact; Advantages of Remote Support); and ‘Psychosocial Experiences’ linked to COVID-19 (Heightened Anxiety; Social Isolation). (4) Conclusions: Women from ethnic minority backgrounds experienced disrupted perinatal mental health care and COVID-19 restrictions compounding their mental health difficulties. Services should take women’s circumstances into account and provide flexibility regarding remote delivery of care.
Does the Formulation of the Decision Problem Affect Retirement?—Framing Effect and Planned Retirement Age
Authors: Jedynak Tomasz
Published: February 2022

The aim of the study is to fill the research gap in relation to one of the behavioral factors that have a potential impact on retirement decisions—the framing effect. A research question addressed in the study is whether the way in which the decision-making problem is formulated (the framing effect) influences decisions on the planned retirement age. To answer this question, an original research questionnaire was developed. It included a description of a hypothetical pension system and experimental vignette questions. The research was conducted on the basis of answers given by 1079 randomly selected respondents who were participants of the pension system in Poland before retirement. In the analysis of the results, non-parametric tests and multiple logistic regression were used to compare response distributions. As a result of the conducted research, it was proven that the framing effect significantly affects the extension of the planned retirement age. At the same time, it was found that loss framing affects pension decisions to a greater extent than gain framing. It has also been noted that women are more susceptible than men to the framing of pension decisions. An application conclusion resulting from the conducted research is indicated as the possibility of the intentional use of the framing effect by decision-makers in order to increase the effective retirement age.
Antimony Immobilization in Primary-Explosives-Contaminated Soils by Fe–Al-Based Amendments
Authors: Wang Ningning , Jiang Yucong , Xia Tianxiang , Xu Feng , Zhang Chengjun , Zhang Dan , Wu Zhiyuan
Published: February 2022

Soils at primary explosives sites have been contaminated by high concentrations of antimony (Sb) and co-occurring heavy metals (Cu and Zn), and are largely overlooked and neglected. In this study, we investigated Sb concentrations and species and studied the effect of combined Fe- and Fe–Al-based sorbent application on the mobility of Sb and co-occurring metals. The content of Sb in soil samples varied from 26.7 to 4255.0 mg/kg. In batch experiments, FeSO4 showed ideal Sb sorption (up to 97% sorption with 10% FeSO4·7H2O), whereas the sorptions of 10% Fe0 and 10% goethite were 72% and 41%, respectively. However, Fe-based sorbents enhanced the mobility of co-occurring Cu and Zn to varying levels, especially FeSO4·7H2O. Al(OH)3 was required to prevent Cu and Zn mobilization. In this study, 5% FeSO4·7H2O and 4% Al(OH)3 mixed with soil was the optimal combination to solve this problem, with Sb, Zn, and Cu stabilizations of 94.6%, 74.2%, and 82.2%, respectively. Column tests spiked with 5% FeSO4·7H2O, and 4% Al(OH)3 showed significant Sb (85.85%), Zn (83.9%), and Cu (94.8%) retention. The pH-regulated results indicated that acid conditioning improved Sb retention under alkaline conditions. However, no significant difference was found between the acidification sets and those without pH regulation. The experimental results showed that 5% FeSO4·7H2O + 4% Al(OH)3 without pH regulation was effective for the stabilization of Sb and co-occurring metals in primary explosive soils.
The Arabic Version of the Tegner Activity Scale in Patients with Anterior Cruciate Ligament Reconstruction: Translation, Validation, and Cross-Cultural Adaptation
Authors: Alzhrani Msaad , Alzahrani Hosam , Alshehri Yasir S.
Published: February 2022

Background: The Tegner activity scale is a patient-reported questionnaire that is frequently used to measure activity levels in patients with anterior cruciate ligament reconstruction (ACLR). The purpose of this study was to translate, cross-culturally adapt, and validate the Tegner activity scale into Arabic. Methods: The Tegner activity scale–Arabic version (TAS-Ar) was forward and backward translated, cross-culturally adapted, and validated according to established guidelines. Seventy-five patients who underwent ACLR were instructed to complete the TAS-Ar, the International Knee Documentation Committee (IKDC) subjective knee evaluation form, and the Knee Injury and Osteoarthritis Outcome Score (KOOS) scale. The test-retest reliability of the TAS-Ar was assessed in 39 patients. Statistical tests were conducted to test the reliability and construct validity of the TAS-Ar. Results: The TAS-Ar showed excellent test-retest reliability, with intraclass correlation coefficients of 0.836 (p < 0.001). The TAS-Ar was significantly correlated with the IKDC (Spearman’s rho = 0.476, p < 0.001), all KOOS subscales (Spearman’s rho = 0.195–0.497, p < 0.05), and the KOOS total score (Spearman’s rho = 0.469, p < 0.001). Conclusions: The Arabic version of the Tegner activity scale is a reliable and valid measure that can be used to evaluate the activity level of Arabic-speaking patients following ACLR.
Indoor Air Radon Concentration in Premises of Public Companies and Workplaces in Latvia
Authors: Reste Jelena , Pavlovska Ilona , Martinsone Zanna , Romans Andris , Martinsone Inese , Vanadzins Ivars
Published: February 2022

Considering the multitudes of people who spend their time working indoors in public premises and workplaces, it is worth knowing what their level of exposure is to natural radioactive radon gas, the second most widespread and dangerous carcinogen for lung cancer development after cigarette smoking. This state-level study covered most of the territory of Latvia and conducted 941 radon measurements with Radtrack2, placed for 4–6 months in the premises of public companies, educational institutions, medical care institutions, etc. The study found that 94.7% of samples did not exceed the national permissible limit (200 Bq/m3), the level at which preventive measures should be initiated. The median value of average specific radioactivity of radon in these premises was 48 Bq/m3 (Q1 and Q3 being 27 and 85 Bq/m3), which is below the average of the European region. Slightly higher concentrations were observed in well-insulated premises with plastic windows and poorer air exchange, mostly in schools (59 (36, 109) Bq/m3) and kindergartens (48 (32, 79) Bq/m3). Industrial workplaces had surprisingly low radon levels (28 (16, 55) Bq/m3) due to strict requirements for air quality and proper ventilation. Public premises and workplaces in Latvia mostly have low radon concentrations in the air, but more attention should be paid to adequate ventilation and air exchange.
Benthic Biofilm Bacterial Communities and Their Linkage with Water-Soluble Organic Matter in Effluent Receivers
Authors: Wang Longfei , Wang Yutao , Li Yi , Zhang Wenlong , Zhang Huanjun , Niu Lihua , Habibul Nuzahat
Published: February 2022

Benthic biofilms are pioneering microbial aggregates responding to effluent discharge from wastewater treatment plants (WWTPs). However, knowledge of the characteristics and linkage of bacterial communities and water-soluble organic matter (WSOM) of benthic biofilms in effluent-receiving rivers remains unknown. Here, we investigated the quality of WSOM and the evolution of bacterial communities in benthic biofilm to evaluate the ecological impacts of effluent discharge on a representative receiving water. Tryptophan-like proteins showed an increased proportion in biofilms collected from the discharge area and downstream from the WWTP, especially in summer. Biofilm WSOM showed weak humic character and strong autochthonous components, and species turnover was proven to be the main factor governing biofilm bacteria community diversity patterns. The bacterial community alpha diversity, interspecies interaction, biological index, and humification index were signally altered in the biofilms from the discharge area, while the values were more similar in biofilms collected upstream and downstream from the WWTP, indicating that both biofilm bacterial communities and WSOM characters have resilience capacities. Although effluent discharge simplified the network pattern of the biofilm bacterial community, its metabolic functional abundance was basically stable. The functional abundance of carbohydrate metabolism and amino acid metabolism in the discharge area increased, and the key modules in the non-random co-occurrence network also verified the important ecological role of carbon metabolism in the effluent-receiving river. The study sheds light on how benthic biofilms respond to effluent discharge from both ecological and material points of view, providing new insights on the feasibility of utilizing benthic biofilms as robust indicators reflecting river ecological health.
Development of a Clinical Risk Score for Prediction of Life-Threatening Arrhythmia Events in Patients with ST Elevated Acute Coronary Syndrome after Primary Percutaneous Coronary Intervention
Authors: Wongthida Thanutorn , Lumkul Lalita , Patumanond Jayanton , Wongtheptian Wattana , Piyayotai Dilok , Phinyo Phichayut
Published: February 2022

ST-elevated acute coronary syndrome (STEACS) is a serious condition requiring timely treatment. Reperfusion with primary percutaneous coronary intervention (pPCI) is recommended and preferred over fibrinolysis. Despite its efficacy, lethal complications, such as life-threatening arrhythmia (LTA), are common in post-PCI patients. Although various risk assessment tools were developed, only a few focus on LTA prediction. This study aimed to develop a risk score to predict LTA events after pPCI. A risk score was developed using a retrospective cohort of consecutive patients with STEACS who underwent pPCI at Chiangrai Prachanukroh Hospital from January 2012 to December 2016. LTA is defined as the occurrence of malignant arrhythmia that requires advanced cardiovascular life support (ACLS) within 72 h after pPCI. Logistic regression was used for model derivation. Among 273 patients, 43 (15.8%) developed LTA events. Seven independent predictors were identified: female sex, hemoglobin < 12 gm/dL, pre- and intra-procedural events (i.e., respiratory failure and pulseless arrest), IABP insertion, intervention duration > 60 min, and desaturation after pPCI. The LTA score showed an AuROC of 0.93 (95%CI 0.90, 0.97). The score was categorized into three risk categories: low (<2.5), moderate (2.5–4), and high risk (>4) for LTA events. The LTA score demonstrated high predictive performance and potential clinical utility for predicting LTA events after pPCI.
Impact of the First Phase of the COVID-19 Pandemic on the Acquisition of Goods and Services in the Italian Health System
Authors: Capuzzo Martina , Viganò Gian Luca , Boniotti Cinzia , Ignoti Lucia Maria , Duri Claudia , Cimolin Veronica
Published: February 2022

The emergency caused by the escalation in the COVID-19 pandemic, which became widespread starting on 31 January 2020, put a strain on the Italian National Health System and forced purchasing centres to deviate from the ordinary general principles dictated by current legislation. The aim of this paper is to describe how Spedali Civili Hospital in Brescia challenged the crisis, structured itself optimally, followed simplified procedures, launched new processes, and opened up more Intensive Care Unit beds to accommodate the high number of COVID cases. From an analysis of the equipment variation in terms of increased purchases, subsequent installations, and tests carried out compared with the pre-pandemic period, we report the difficulties that hospitals had to face in the first phase of the pandemic and how they were able to respond to their needs. Our data clearly displayed how the pandemic situation led to a deep internal reorganisation and that the drafting of simpler, effective, and adaptable procedures represents a first key element to ensure receptivity and responsiveness in the management of ordinary and non-ordinary events such as this pandemic condition.
Patient Participation and the Environment: A Scoping Review of Instruments
Authors: Kylén Maya , Schön Ulla-Karin , Pessah-Rasmussen Hélène , Elf Marie
Published: February 2022

Patient participation and the environment are critical factors in achieving qualitative healthcare. We conducted a systematic scoping review using Arksey and O’Malley’s framework to identify instruments intended to measure patient participation. We assessed those instruments’ characteristics, which areas of the healthcare continuum they target, and whether environmental factors are considered. Instruments were considered eligible if they represented the patient perspective and measured patient participation in healthcare. The search was limited to articles written in English and published in the last 10 years. We extracted concepts (i.e., patient empowerment, patient participation, and patient-centeredness) based on the framework developed by Castro et al. and outcomes of significance regarding the review questions and specific objectives. The search was conducted in PsycINFO, CINHAL/EBSCO, and PubMed in September 2019 and July 2020. Of 4802 potential titles, 67 studies reported on a total of 45 instruments that met the inclusion criteria for this review. The concept of patient participation was represented most often in these studies. Although some considered the social environment, no instrument was found to incorporate and address the physical environment. Thirteen instruments were generic and the remaining instruments were intended for specific diagnoses or healthcare contexts. Our work is the first to study instruments from this perspective, and we conclude that there is a lack of instruments that measure aspects of the social and physical environment coherently as part of patient participation.
Toward Gaze-Based Map Interactions: Determining the Dwell Time and Buffer Size for the Gaze-Based Selection of Map Features
Authors: Liao Hua , Zhang Changbo , Zhao Wendi , Dong Weihua
Published: February 2022

The modes of interaction (e.g., mouse and touch) between maps and users affect the effectiveness and efficiency of transmitting cartographic information. Recent advances in eye tracking technology have made eye trackers lighter, cheaper and more accurate, broadening the potential to interact with maps via gaze. In this study, we focused exclusively on using gaze to choose map features (i.e., points, polylines and polygons) via the select operation, a fundamental action preceding other operations in map interactions. We adopted an approach based on the dwell time and buffer size to address the low spatial accuracy and Midas touch problem in gaze-based interactions and to determine the most suitable dwell time and buffer size for the gaze-based selection of map features. We conducted an experiment in which 38 participants completed a series of map feature selection tasks via gaze. We compared the participants’ performance (efficiency and accuracy) between different combinations of dwell times (200 ms, 600 ms and 1000 ms) and buffer sizes (point: 1°, 1.5°, and 2°; polyline: 0.5°, 0.7° and 1°). The results confirmed that a larger buffer size raised efficiency but reduced accuracy, whereas a longer dwell time lowered efficiency but enhanced accuracy. Specifically, we found that a 600 ms dwell time was more efficient in selecting map features than 200 ms and 1000 ms but was less accurate than 1000 ms. However, 600 ms was considered to be more appropriate than 1000 ms because a longer dwell time has a higher risk of causing visual fatigue. Therefore, 600 ms supports a better balance between accuracy and efficiency. Additionally, we found that buffer sizes of 1.5° and 0.7° were more efficient and more accurate than other sizes for selecting points and polylines, respectively. Our results provide important empirical evidence for choosing the most appropriate dwell times and buffer sizes for gaze-based map interactions.
Root Membrane Ubiquitinome under Short-Term Osmotic Stress
Authors: Berger Nathalie , Demolombe Vincent , Hem Sonia , Rofidal Valérie , Steinmann Laura , Krouk Gabriel , Crabos Amandine , Nacry Philippe , Verdoucq Lionel , Santoni Véronique
Published: February 2022

Osmotic stress can be detrimental to plants, whose survival relies heavily on proteomic plasticity. Protein ubiquitination is a central post-translational modification in osmotic-mediated stress. In this study, we used the K-Ɛ-GG antibody enrichment method integrated with high-resolution mass spectrometry to compile a list of 719 ubiquitinated lysine (K-Ub) residues from 450 Arabidopsis root membrane proteins (58% of which are transmembrane proteins), thereby adding to the database of ubiquitinated substrates in plants. Although no ubiquitin (Ub) motifs could be identified, the presence of acidic residues close to K-Ub was revealed. Our ubiquitinome analysis pointed to a broad role of ubiquitination in the internalization and sorting of cargo proteins. Moreover, the simultaneous proteome and ubiquitinome quantification showed that ubiquitination is mostly not involved in membrane protein degradation in response to short osmotic treatment but that it is putatively involved in protein internalization, as described for the aquaporin PIP2;1. Our in silico analysis of ubiquitinated proteins shows that two E2 Ub-conjugating enzymes, UBC32 and UBC34, putatively target membrane proteins under osmotic stress. Finally, we revealed a positive role for UBC32 and UBC34 in primary root growth under osmotic stress.
Metastasising Fibroblasts Show an HDAC6-Dependent Increase in Migration Speed and Loss of Directionality Linked to Major Changes in the Vimentin Interactome
Authors: Evans Caroline A. , Kim Hyejeong Rosemary , Macfarlane Sarah C. , Nowicki Poppy I. A. , Baltes Carsten , Xu Lei , Widengren Jerker , Lautenschläger Franziska , Corfe Bernard M. , Gad Annica K. B.
Published: February 2022

Metastasising cells express the intermediate filament protein vimentin, which is used to diagnose invasive tumours in the clinic. We aimed to clarify how vimentin regulates the motility of metastasising fibroblasts. STED super-resolution microscopy, live-cell imaging and quantitative proteomics revealed that oncogene-expressing and metastasising fibroblasts show a less-elongated cell shape, reduced cell spreading, increased cell migration speed, reduced directionality, and stronger coupling between these migration parameters compared to normal control cells. In total, we identified and compared 555 proteins in the vimentin interactome. In metastasising cells, the levels of keratin 18 and Rab5C were increased, while those of actin and collagen were decreased. Inhibition of HDAC6 reversed the shape, spreading and migration phenotypes of metastasising cells back to normal. Inhibition of HDAC6 also decreased the levels of talin 1, tropomyosin, Rab GDI β, collagen and emilin 1 in the vimentin interactome, and partially reversed the nanoscale vimentin organisation in oncogene-expressing cells. These findings describe the changes in the vimentin interactome and nanoscale distribution that accompany the defective cell shape, spreading and migration of metastasising cells. These results support the hypothesis that oncogenes can act through HDAC6 to regulate the vimentin binding of the cytoskeletal and cell–extracellular matrix adhesion components that contribute to the defective motility of metastasising cells.
Lysophosphatidic Acid Promotes the Expansion of Cancer Stem Cells via TRPC3 Channels in Triple-Negative Breast Cancer
Authors: Hirata Naoya , Yamada Shigeru , Yanagida Shota , Ono Atsushi , Yasuhiko Yukuto , Nishida Motohiro , Kanda Yasunari
Published: February 2022

Triple-negative breast cancer (TNBC) is a highly aggressive cancer for which targeted therapeutic agents are limited. Growing evidence suggests that TNBC originates from breast cancer stem cells (BCSCs), and elucidation of the molecular mechanisms controlling BCSC proliferation will be crucial for new drug development. We have previously reported that the lysosphingolipid sphingosine-1-phosphate mediates the CSC phenotype, which can be identified as the ALDH-positive cell population in several types of human cancer cell lines. In this study, we have investigated additional lipid receptors upregulated in BCSCs. We found that lysophosphatidic acid (LPA) receptor 3 was highly expressed in ALDH-positive TNBC cells. The LPAR3 antagonist inhibited the increase in ALDH-positive cells after LPA treatment. Mechanistically, the LPA-induced increase in ALDH-positive cells was dependent on intracellular calcium ion (Ca2+), and the increase in Ca2+ was suppressed by a selective inhibitor of transient receptor potential cation channel subfamily C member 3 (TRPC3). Moreover, IL-8 production was involved in the LPA response via the activation of the Ca2+-dependent transcriptional factor nuclear factor of activated T cells. Taken together, our findings provide new insights into the lipid-mediated regulation of BCSCs via the LPA-TRPC3 signaling axis and suggest several potential therapeutic targets for TNBC.
Inflammation Is Present, Persistent and More Sensitive to Proinflammatory Triggers in Celiac Disease Enterocytes
Authors: Porpora Monia , Conte Mariangela , Lania Giuliana , Bellomo Claudia , Rapacciuolo Luciano , Chirdo Fernando Gabriel , Auricchio Renata , Troncone Riccardo , Auricchio Salvatore , Barone Maria Vittoria , Nanayakkara Merlin
Published: February 2022

Celiac disease (CD) is a chronic inflammatory disease caused by a genetic predisposition to an abnormal T cell-mediated immune response to the gluten in the diet. Different environmental proinflammatory factors can influence and amplify the T cell-mediated response to gluten. The aim of this manuscript was to study the role of enterocytes in CD intestinal inflammation and their response to different proinflammatory factors, such as gliadin and viruses. Intestinal biopsies from CD patients on a gluten-containing (GCD-CD) or a gluten-free diet (GFD-CD) as well as biopsies from potential CD patients (Pot-CD) before the onset of intestinal lesions and controls (CTR) were used to investigate IL-1β and IL-6 mRNA levels in situ. Organoids from CD patients were used to test the levels of NF-κB, ERK, IL-6, and IL-1β by Western blot (WB), ELISA, and quantitative PCR. The Toll-like receptor ligand loxoribine (Lox) and gliadin peptide P31-43 were used as proinflammatory stimuli. In CD biopsies inflammation markers IL-1β and IL-6 were increased in the enterocytes, and also in Pot-CD before the onset of the intestinal lesion and in GFD-CD. The inflammatory markers pNF-κB, pERK, IL-1β, and IL-6 were increased and persistent in CD organoids; these organoids were more sensitive to P31-43 and Lox stimuli compared with CTR organoids. Taken together, these observations point to constitutive inflammation in CD enterocytes, which are more sensitive to inflammatory stimuli such as food components and viruses.
Filtration of Nutritional Fluids in the German Wasp Vespula germanica (Vespidae, Hymenoptera)
Authors: Kuba Kenneth , Krenn Harald W.
Published: February 2022

The mouthparts of Vespidae have evolved to forage various solid and liquid foods, such as animal prey, carbohydrate-rich fluids, as well as woody fibres for nest construction. Before nutritional fluids are ingested into the crop, bigger particles need to be filtered out. This study examined the functional morphology of the mouthparts, the preoral cavity, and the proximal alimentary tract inside the head focusing on this filtration process. The feeding organs and preoral cavity were studied using µCT and SEM that were complimented by feeding experiments with glass beads in workers of Vespula germanica. To visualize fluid ingestion into the head and alimentary tract, barium sulfate solution was used as contrast agent; a method that is rarely applied in entomology. Experimental results indicate that large glass beads (>212 µm) were filtered by the mouthpart structures before entering the preoral cavity. Smaller glass beads (152–212 µm) were found inside the infrabuccal pocket in front of the mouth. Morphological evidence indicates that cuticle structures of the epipharynx, hypopharynx, and cibarium filter this particle size inside the preoral cavity while glass beads < 152 µm reach the crop. A double fluid filtration system is proposed that is formed by (1) bristles of the mouthparts and (2) microtrichia of the preoral cavity.
A Review of Termite Species and Their Distribution in Thailand
Authors: Lertlumnaphakul Watthanasak , Ngoen-Klan Ratchadawan , Vongkaluang Charunee , Chareonviriyaphap Theeraphap
Published: February 2022

Although 3105 termite species have been documented worldwide, little information is available on those in Thailand. In this review, the Google Scholar search platform and the Scopus and Science Direct databases were used to obtain information on termite species and for georeferencing. The QGIS software was used to create point localities that were overlaid on the Thailand administrative level 1 (province) to map the distribution of termite species in the country based on the World Geodetic System 1984. From the 19 reviewed sources, 44 defined coordinates were identified in 14 provinces across Thailand. Among these 44 coordinates, we found 75 termite species and 83 unknown species of termites; in total, 36 termite species were from the North (6 locations), 33 species were from the Northeast (10 locations), 34 species were from the West (4 locations), 29 species were from the Central region (3 locations), 44 species were from the East (8 locations), and 54 species were from the South (13 locations). The most predominant species in all regions were Globitermes sulphureus, Macrotermes gilvus, Microcerotermes crassus, and Microtermes obesi.
Temporal Evaluation of Insecticide Resistance in Populations of the Major Arboviral Vector Aedes Aegypti from Northern Nigeria
Authors: Mukhtar Muhammad M. , Ibrahim Sulaiman S.
Published: February 2022

To support evidence-based control measures, two Nigerian Aedes populations (BUK and Pantami) were characterised. Larval bioassay using temephos and deltamethrin revealed a significant increase in deltamethrin resistance, with LC50 of 0.018mg/L (resistance ratio compared to New Orleans, RR = 2.250) in 2018 increasing ~6-fold, by 2019 (LC50 = 0.100mg/L, RR = 12.5), and ~11-fold in 2020 (LC50 = 0.198mg/L, RR = 24.750). For the median deltamethrin concentration (0.05mg/L), a gradual decrease in mortality was observed, from 50.6% in 2018, to 44.9% in 2019, and 34.2% in 2020. Extremely high DDT resistance was observed, with <3% mortalities and LT50s of 352.87 min, 369.19 min and 406.94 min in 2018, 2019 and 2020, respectively. Significant temporal increase in resistance was observed towards ƛ-cyhalothrin (a type II pyrethroid) over three years. Synergist bioassays with diethylmaleate and piperonylbutoxide significantly recovered DDT and ƛ-cyhalothrin susceptibility respectively, implicating glutathione S-transferases and CYP450s. Cone bioassays revealed increased resistance to the PermaNet® 3.0, side panels (mortalities of 94% in 2018, 66.4% in 2019, and 73.6% in 2020), while full susceptibility was obtained with the roof of PermaNet® 3.0. The F1534C kdr mutation occurred in low frequency, with significant correlation between heterozygote genotypes and DDT resistance. This temporal increase in resistance is a major challenge for control of this vector of public health importance.
What the near Future Holds for Sacubitril/Valsartan: A Summary of Major Ongoing Studies
Authors: Badreldin Hisham A. , Aldosari Nasser , Alnashwan Lama , Almutairi Taif , Yousif Nada , Alsulaiman Khalid , Aljuhani Ohoud , Hafiz Awatif , Alshaya Omar
Published: February 2022

Early research on neprilysin inhibition showed that sacubitril/valsartan, a combination of the valsartan and the neprilysin inhibitor sacubitril, was superior to enalapril in patients with heart failure with reduced ejection fraction (HFrEF) in the PARADIGM-HF study in 2014. Therefore, for patients with HFrEF, worldwide recommendations have been reformed to include sacubitril/valsartan. In addition, sacubitril/valsartan has been investigated in other cardiovascular disease states, such as patients with heart failure and preserved ejection fraction (HFpEF) and following myocardial infarction (MI) events. In February 2021, the FDA expanded the indication use of sacubitril/valsartan to include the HFpEF patient population based on the results of the PARAGON-HF trial. However, randomized clinical trials post-MI did not show promising results. Sacubitril/valsartan is currently being investigated in many other cardiovascular and non-cardiovascular conditions. This review aims to shed light and summarize the ongoing sacubitril/valsartan registered studies on the United States National Library of Medicine clinical trials registry.
The Dilemma of the Level of the Inferior Mesenteric Artery Ligation in the Treatment of Diverticular Disease: A Systematic Review of the Literature
Authors: Cirocchi Roberto , Mari Giulio , Amato Bruno , Tebala Giovanni Domenico , Popivanov Georgi , Avenia Stefano , Nascimbeni Riccardo
Published: February 2022

Background and aim: Although sigmoidectomy is a well-standardized procedure for diverticular disease, there are still unclear areas related to the varying morphology and vascular supply of the sigmoid colon. The level of vascular ligation could affect the functional outcomes of patients operated on for diverticular disease. The aim of this review is to primarily evaluate sexual, urinary and defecatory function outcomes, as well as postoperative results, in patients who underwent surgery for diverticular disease, with or without inferior mesenteric artery (IMA) preservation. Materials and methods: The MEDLINE/PubMed, WOS and Scopus databases were interrogated. Comparative studies including patients who underwent sigmoidectomy for diverticular diseases were considered. Bowel function, genitourinary function, anastomotic leak, operation time, conversion to open surgery, anastomotic bleeding, bowel obstruction were the main items of interest. Results: Twelve studies were included in the review, three randomized and nine comparative studies. Bowel and genitourinary function are not differently affected by the level of vascular ligation. The site of ligation of IMA did not influence the rate of functional complications, anastomotic leak and bleeding. Of note, the preservation of IMA is associated with a higher conversion rate and longer operative time. Conclusions: Despite the heterogeneity of patient groups, and although the findings should be interpreted with caution, functional and clinical outcomes after sigmoidectomy for diverticular disease do not seem to be affected by the level of vascular ligation as long as the IMA is ligated far from its origin.
Reply to Weiss, H.-R. Comment on “Costa et al. The Effectiveness of Different Concepts of Bracing in Adolescent Idiopathic Scoliosis (AIS): A Systematic Review and Meta-Analysis. J. Clin. Med. 2021, 10, 2145”
Authors: Costa Lorenzo , Schlösser Tom P. C. , Kruyt Moyo C. , Castelein René M.
Published: February 2022

We would like to thank you for the opportunity to reply to the comments in regard of the letter by Dr. Weiss [...]
The Role of Thyroid Disorders, Obesity, Diabetes Mellitus and Estrogen Exposure as Potential Modifiers for Pulmonary Hypertension
Authors: Vrigkou Eleni , Vassilatou Evangeline , Dima Effrosyni , Langleben David , Kotanidou Anastasia , Tzanela Marinella
Published: February 2022

Pulmonary hypertension (PH) is a progressive disorder characterized by a chronic in-crease in pulmonary arterial pressure, frequently resulting in right-sided heart failure and potentially death. Co-existing medical conditions are important factors in PH, since they not only result in the genesis of the disorder, but may also contribute to its progression. Various studies have assessed the impact of thyroid disorders and other endocrine conditions (namely estrogen exposure, obesity, and diabetes mellitus) on the progression of PH. The complex interactions that hormones may have with the cardiovascular system and pulmonary vascular bed can create several pathogenetic routes that could explain the effects of endocrine disorders on PH development and evolution. The aim of this review is to summarize current knowledge on the role of concomitant thyroid disorders, obesity, diabetes mellitus, and estrogen exposure as potential modifiers for PH, and especially for pulmonary arterial hypertension, and to discuss possible pathogenetic routes linking them with PH. This information could be valuable for practicing clinicians so as to better evaluate and/or treat concomitant endocrine conditions in the PH population.
Dissemination of Chest Compression-Only Cardiopulmonary Resuscitation by Bystanders for Out-of-Hospital Cardiac Arrest in Students: A Nationwide Investigation in Japan
Authors: Kiyohara Kosuke , Kitamura Yuri , Ayusawa Mamoru , Nitta Masahiko , Iwami Taku , Nakata Ken , Sobue Tomotaka , Kitamura Tetsuhisa
Published: February 2022

We aimed to investigate how the types of bystander-initiated cardiopulmonary resuscitation (CPR) for out-of-hospital cardiac arrest (OHCA) among students have changed recently. We also determined the association between two types of bystander-CPRs (i.e., chest compression-only CPR [CCCPR] and conventional CPR with rescue breathing [CCRB]) and survival after OHCA. From a nationwide registry of pediatric OHCAs occurring in school settings in Japan, the data of 253 non-traumatic OHCA patients (elementary, junior high, and high school/technical college students) receiving bystander-CPR between April 2008 and December 2017 were analyzed. Multivariable logistic regression analysis was conducted to assess the impact of different types of bystander-CPR on 30-day survival with favorable neurological outcomes. The proportion of patients receiving CCCPR increased from 25.0% during 2008–2009 to 55.3% during 2016–2017 (p for trend < 0.001). Overall, 53.2% (50/94) of patients receiving CCCPR and 46.5% (74/159) of those receiving CCRB survived for 30 days with favorable neurological outcomes. Multivariable analysis showed no significant difference in outcomes between the two groups (adjusted odds ratio: 1.23, 95% confidence interval: 0.67–2.28). In this setting, CCCPR is a common type of bystander-CPR for OHCA in students, and the effectiveness of CCCPR and CCRB on survival outcomes seems comparable.
A Molecular Dynamics Study of the Stability and Mechanical Properties of a Nano-Engineered Fuzzy Carbon Fiber Composite
Authors: Almousa Hassan , Peng Qing , Alsayoud Abduljabar Q.
Published: February 2022

Carbon fiber-reinforced polymer composites are used in various applications, and the interface of fibers and polymer is critical to the composites’ structural properties. We have investigated the impact of introducing different carbon nanotube loadings to the surfaces of carbon fibers and characterized the interfacial properties by molecular dynamics simulations. The carbon fiber (CF) surface structure was explicitly modeled to replicate the graphite crystallites’ interior consisting of turbostratic interconnected graphene multilayers. Then, single-walled carbon nanotubes and polypropylene chains were packed with the modeled CFs to construct a nano-engineered “fuzzy” CF composite. The mechanical properties of the CF models were calculated through uniaxial tensile simulations. Finally, the strength to peel the polypropylene from the nano-engineered CFs and interfacial energy were calculated. The interfacial strength and energy results indicate that a higher concentration of single-walled carbon nanotubes improves the interfacial properties.