bluuebunny/crossref_metadata_2025 huggingface dataset:
Identifikasi Salmonella, Shigella dan E. coli pada Sie Balu, Bahan Pangan Olahan Asal Daging
Authors: Husna Husna
Published: June 2020

Sie balu merupakan salah satu produk tradisional Aceh yang  diolah dari bahan pangan asal hewan yaitu daging. Sie balu diproses dengan penambahan bumbu dan dikeringkan. Proses pengolahan yang lama dan pengeringan di bawah sinar matahari langsung dapat menyebabkan produk daging terkontaminasi mikroba. Penelitian ini bertujuan mengetahui keberadaan Salmonella, Shigella dan E. coli pada sie balu. Sie balu dibuat dari 5 kg daging sapi segar, dijemur hingga kering. Salmonella, Shigella dan E. coli diidentifikasi menggunakan uji bakteriologis dan biokimia. Data dianalisis secara deskriptif. Hasil penelitian menunjukkan sie balu terkontaminasi oleh Salmonella, Shigella dan E. coli. Disarankan pengolahan sie balu sebaiknya memperhatikan proses higiene dan sanitasi dalam setiap tahapan pengolahan dan metode pengeringan sie balu menggunakan peralatan yang tertutup.
Methylation Related To Benefit And Harm In RNAI Application: An Epigenetic Quasi Systematic Review
Authors: Samsuria Peni Kistijani , Samsuria Indranila Kustarini
Published: June 2020

Pendahuluan: Latar belakang: RNA untai ganda (dsRNA), siRNA, miRNA, RNAi, menginduksi metilasi DNA pada tumbuhan dan dalam sel mamalia, termasuk manusia. Kini RNAi menjadi prinsip dalam pengobatan kanker sekarang dan di masa depan. Masalah: Metilasi pulau CpG dan pengulangan DNA CGG merupakan kasus prevalensi tinggi di hutan hujan tropis, tetapi sampai sekarang terabaikan. Prinsip kehati-hatian dalam efek lingkungan harus diambil oleh para pembuat keputusan dan pemegang kebijakan. Tujuan: Mengetahui efek pembungkaman gen terhadap lingkungan. Hipotesis: RNAi menyebabkan hipermetilasi. Metode: Quasi ‘Systematic Review’ dengan Analisis Bayesian. Hasil: Menggunakan mesin pencari Science Direct, 935 referensi tertangkap ditambah 11 referensi yang sudah direkam dalam pustaka Mendeley, dan setelah menyaring abstrak atau judul, 920 dikeluarkan dengan duplikasi yang tidak relevan baik dianalisis dengan jaringan Bayesian terbaru untuk menjawab hipotesis. Menyaring teks lengkap dari 18, kemudian 16 teks lengkap dipilih. 28 teks lengkap diperiksa dan periksa kembali dengan meta-analisis RNA-metilasi menggunakan Science Direct (12 referensi). Diskusi: Sebuah teknik CpG-siRNA telah mempertahankan hipermetilasi pulau CpG, digunakan secara luas dalam terapi tanaman dan kanker untuk menstabilkan gen yang terbungkam.Kesimpulan: Pengaruh teknik pembungkaman gen terhadap lingkungan harus diketahui secara luas oleh pemegang kebijakan dan pengambil keputusan. Keywords: Hipermetilasi, Pulau CpG, RNAi, Pembungkaman gen, Budidaya ikan
ASSOCIATION BETWEEN NUTRITIONAL STATUS AND THE OUTCOME OF PEDIATRIC PATIENT WITH DENGUE SHOCK SYNDROME
Authors: Pramudito Shidi Laras , Sari Dewi Ratna , Soemyarso Ninik Asmaningsih
Published: June 2020

Background: The association between nutritional status and dengue infection is still considered controversial. Researches that study obesity and shock in pediatric patients with dengue hemmorhagic fever (DHF) has been widely carried out. However, researches that study the association between obesity and the outcome of patients with dengue shock syndrome (DSS) are still rarely done. Objective: The objective is to study the association of obesity in body mass index-for-age (BMI-for-age), gender, and age to the outcome of patients with dengue shock syndrome (recurrent shock or death). Material and method: The research was analytical retrospective observational research with a case-control design using secondary data from the medical records of pediatric patients with DSS in Dr. Soetomo General Hospital during 2015. The analyses used to test the association of obesity (BMI-for-age), gender, age and the outcome of patients with dengue shock syndrome (recurrent shock or death) were the Chi Square or Fisher Exact test. Result: The sample acquired was 60 data, consisting of 46 patients with DSS without recurrent shock or death and 14 pediatric patients with DSS experiencing recurrent shock or mortality. The frequency of boys and girls were found in equal numbers. Age group >5-10 years was found more common (45%). The results of the analyses test found no asssiation between gender (p=0.542), age (p=0.314), and obesity (p=1,000) and the outcome of pediatric patients with dengue shock syndrome. Conclusion: There were no association found between obesity, age, and gender to the outcomes of pediatric patients with DSS at Dr. Soetomo General Hospital.
BODY MASS INDEX (BMI) OF CHILDREN WITH TETRALOGY OF FALLOT (TOF)
Authors: Saputri Ovin Nada , Kalanjati Viskasari P. , Rahman Mahrus A.
Published: June 2020

Background: Malnutrition is a common cause of morbidity and mortality amongst children with TOF. Objective: To analyze the BMI profile of children with TOF age 0 – 18 years old at Pediatric Department, Dr. Soetomo General Hospital, Surabaya. Material and method: A cross-sectional study of the BMI from the medical record of TOF patients age 0 – 18 years old at the Pediatric Department, Dr. Soetomo General Hospital, Surabaya since January 2016 to December 2017 was conducted. The data included gender, age group, demographic distribution, weight, and height. BMI was calculated by WHO formula reference; body weight (kg) divided by height squared body (m2), which was converted into z-score histogram size. BMI classification was guided by the Government’s Anthropometry Standards for Nutritional Status Assessment. Result: From 84 TOF patients, there were 43 males (51%) and 41 females (49%). The most dominant age group is 0 – 4 years old (n=47;56%). The majority of patients were from outside of Surabaya (n=64;76,2%). Based on their BMI, 36 patients (42.9%) have severe underweight, 10 patients (11.9%) were underweight, and 35 patients (41.7%) were normal, whilst the rests (3.6%) were overweight. Conclusion: In this study, the number of male patients was slightly higher than female patients. The majority of TOF patients were 0 – 4 years old and resided out town of Surabaya. The predominant BMI found was the underweight and severe underweight below of normal BMI.
PENGARUH PEMBERIAN EKSTRAK KULIT SEMANGKA (Citrullus lanatus) TERHADAP MOTILITAS DAN VIABILITAS SPERMATOZOA TIKUS (Rattus norvegicus) DENGAN PAPARAN SUHU PANAS
Authors: Rohmah Lailatul , Triana Indah Norma , Sunarso Agus , Susilowati Suherni , Hidajati Nove , Kurnijasanti Rochmah
Published: June 2020

High temperatures lead to oxidative stress which can decrease the quality of spermatozoa. Watermelon’s rind have an ability for antioxidants effect that can reduce sperm’s damages that results by role explanation of free radicals. This study aimed to determine the effect of extract of watermelon’s rind on the motility and viability of sperm on male rat exposed to heat. This experiments use rat as animals experiment with five treatments and four repetitions. Five treatments are: P0: without exposured to 40˚C of temperature and without treatment by extract watermelon’s rind, P1: was exposured to 40˚C of temperature and without treatment by extract watermelon’s rind, P2: was exposured to 40˚C of temperature and treatments by extract watermelon’s rind dosage 20 mg/rat/day, P3: was exposured to 40˚C of temperature and treatments by extract watermelon’s rind dosage 40 mg/rat/day, and P4: was exposured to 40˚C of temperature and treatments by extract watermelon’s rind dosage 80 mg/rat/day. The treatment was done for 52 days. The results, P4 have the best effect that can be increase the motility and viability of rat’s sperm (Rattus norvegicus) exposed to heat.
CONCEPTION RATE DAN SERVICE PER CONCEPTION PADA SAPI PERAH AKSEPTOR INSEMINASI BUATAN DI KUD ARGOPURO KECAMATAN KRUCIL KABUPATEN PROBOLINGGO
Authors: Yohana Nadia , Samik Abdul , Aksono Bimo , Sardjito Trilas , Hermadi Herry Agoes , Restiadi Tjuk Imam
Published: June 2020

Reproduction is one of essential factor directly affecting profitability in a dairy production system. Some of the reproductive factors that influenced to the developing of dairy cattle of small holder’s farmer are Conception Rate (CR) Service per Conception (S/C). The study was conducted to know the CR and S/C of Friesian Holstein in KUD Argopuro, Probolinggo. The data is taken primary and secondary data. Primary data retrieval is done by direct observation, which includes several variables, where the variables include : the identity of the breeder, cages, and feeding and drinking. As for the secondary data obtained by recording the card Artificial Insemination (AI) owned by Inseminator in KUD Argopuro. The data which by collected from this sample are CR and S/C. This research was a case study. The materials used cattle as the acceptors were 10% from 728 lactation cattle. Data from the results of this research on CR (30%) and S/C (2,49). The conclusion of this research for CR the result were lower than normal and S/C the result were higher than normal.
UJI BERBAGAI ALAT DETEKSI BIRAHI TERHADAP ANGKA KEBUNTINGAN PADA SAPI PERAH DI KOPERASI TUNAS SETIA BARU KABUPATEN PASURUAN
Authors: Andriyanti Silvia Rani , Hariadi Mas’ud , Darsono Roesno , Srianto Pudji , Wurlina Wurlina , Restiadi Tjuk Imam
Published: June 2020

This research aims to determine the use of various estrus detection devices before artificial insemination as measured by pregnancy rate. The researcher used a sample of 60 cows were used in this research which is divided into four groups as a control and treatment groups (Thermopin®, Hauptner and Draminski groups), and each cow was detected for the sign of estrus by three devices that were Thermopin®, Hauptner and Draminski prion when doing an artificial insemination. The cows were observed to 45 days after artificial insemination for observing pregnancy. This research method is the exploration scope to prove the effect of various estrus detection tool against pregnancy rates. The result of ANOVA test, there is the effect of using a various estrus detection devices against pregnancy rates. Samples tested by Draminski with average (1.53±0.51) resulted in a 53,3% pregnancy rate, the samples were tested with Hauptner with average (1.67±0,48) resulted in pregnancy rate 66,7%. Without treatment or control, sample with an average of (1.67±0,48) resulted in a  73,3% pregnancy rate. While samples were tested with Thermopin® with average (2.00±0,00) resulted in a 100% pregnancy rate. In conclusion, Thermopin® the best detection tool than other estrus detection devices.
EFISIENSI REPRODUKSI SAPI PERANAKAN LIMOUSIN AKSEPTOR INSEMINASI BUATAN DI KECAMATAN TIKUNG, KABUPATEN LAMONGAN TAHUN 2016
Authors: Prastyorini Vina Ari , Supranianondo Koesnoto , Safitri Erma , Restiadi Tjuk Imam , Wurlina Wurlina , Hernawati Tatik
Published: June 2020

The purpose of this study was known the difference of reproductive performans Limousine Cross Breed that haved artificial insemination from diffrence straw from BBIB Singosari and BBIB Lembang covering Conception Rate, Service per Conception, and Calving Rate in period 2016th. This study uses survey research which data from primary and secondary. Primary data were obtained from interview with Limousine Cross Breed breeders and secondary data were obtained from artificial insemination officer’s record. The observed variables are Conception rate (CR),  Service per conception (S/C) and Calving rate  (CvR). Data were analyzed descriptive that average and Chi Square.Result of research showed no significant difference (p>0.05) on CR, S/C and CvR between Limousin cross breed cows that haved artificial insemination from BBIB Singosari and BIB Lembang.
PROFIL PROTEIN TIROSIN KINASE DALAM SEMINAL PLASMA DOMBA MERINO DENGAN TEKNIK SODIUM DODECYL SULPHATE POLYACRILAMIDE GEL ELECTROPHORESIS
Authors: Nawangwulan Tantri , Madyawati Sri Pantja , Plumeriastuti Hani , Sardjito Trilas , Susilowati Suherni , Safitri Erma
Published: June 2020

The aim of this study was to find out protein tyrosine kinase profile in Merino Sheep seminal plasma. This study consist of collecting semen of Merino Sheep containing plasma and spermatozoa, the separation between seminal plasma and spermatozoa, then tyrosine kinase analysis using Sodium Dodecyl Sulphate Polyacrilamide Gel Electrophoresis (SDS-PAGE). Semen was collected by artificial vagina and then centrifugated for 40 minutes at 4000 rpm to separated seminal plasma and spermatozoa. Protein was analyzed using SDS-PAGE, to separate each protein based on their molecular weight. The result showed that there were 13 protein bands in 3 seminal plasma samples with an average 149,63 kDa, 139,7 kDa, 114,97 kDa, 109,3 kDa, 97,33 kDa, 93,83 kDa, 86,23 kDa, 77,6 kDa, 64,6 kDa, 52,3 kDa, 41,93 kDa, 38,13 kDa, and 34,5 kDa. In conclusion, it is believed that tyrosine kinase located on the sixth band with molecular weight 93,83 kDa.
PENGARUH PEMBERIAN MADU TERHADAP STAGING SPERMATOGENESIS DAN SEL LEYDIG PADA MENCIT (Mus musculus) YANG DIINFEKSI Toxoplasma gondii
Authors: Fitri Paraswita Eindah , Wurlina Wurlina , Chusniati Sri , Suwanti Lucia Tri , Mufasirin Mufasirin , Plumeriastuti Hani
Published: June 2020

The aim of this research is to know about the staging of spermatogenesis and leydig cells in mice that injected by Toxoplasma gondii after giving honey as a preventive substance. This research used 25 mice in age 2-4 month with 20-30 weight. Mice were divided into 5 groups (P0, P1, P2, P3 dan P4). P0 as a control teratment were given aquades in peroral, P1 was a group given honey in doses I (0.08ml/0.2ml/day) during 14 days in peroral, P2 was a group that given infection by T.gondii in doses 103 in peritonial, P3 was given honey in doses I 0.08ml/0.2ml/day) then injected by T.gondii, and P4 was given honey in doses II (0.12ml/0.2ml/day) then injected by T.gondii. This research conducted in 18 days, in day 14th mice were given honey and the day 15th–18th mice injected by T.gondii doses 103. The data result was analyzed with Analysis of Variant (ANOVA) and cotinued with Duncan. Comparision statistical showed with SPSS statistic 20 for Windows. The result of analysis, P0 and P1 were significantly different (p<0.05), with the increase number of spermatogenic and leydig cells. P0 and P2 also significantly different, with the decrease number of spermatogenic and leydig cells. In P2 and P3 group were not significantly different (P>0.05), also in P4 group were not significantly different with P0 and P1. The results of this research showed that honey in doses II can maintain the number of sprematogenic and leydig cells as a preventive substance against T.gondii.
PROFIL GEN RECEPTOR GROWTH HORMONE (rGH) PADA SAPI MADRASIN
Authors: Arganata Gigih Lesmana , Utomo S Budi , R.T.S Adikara R.T.S Adikara
Published: June 2020

Cross-breeding between Madura cattle and exotic breeds (exotic cows) occurs in Madura area. The reality in the field shows that the cross-breeding occurs between Limousin cow and Madura cow and the result of the breeding is called as Madrasin cow. One of factor that influences the animal’s growth is Growth Hormone Receptor (GHR).  GHR is needed by Growth Hormone (GH) to carry out its effects on target tissues. The purpose of this study is to identify the profile of Growth Hormone Receptors in Madrasin cow. This research was conducted on farmers in Bangkalan, East Java and the Center of Veterinary, Denpasar in MayJune 2017. This study used 14 cows Madrasin. The samples of this study were blood which were taken and used for analyzing DNA that includes DNA isolation, DNA amplification by using Polymerase Chain Reaction (PCR) and Restriction Fragment Length Polymorphism (RFLP) method to determine the genotype. An identification of Growth Hormone Receptor gene polymorphism is done through digesting the DNA fragment of 298 bp by the restriction enzyme AluI. The results shows that the frequency of Growth Hormone Receptor Gene of  cow Madrasin allele A was 0.92 and the G allele was 0.08 with frequency of the A allele is higher than G allele.The genotype changing was found in cow Madrasin as much as 0.85 to 0.14 for the AA and AG. As a result of the study, the researcher concluded that Growth Hormone Receptor gene experienced a changing or polymorphisms in Madrasin cow and information which stated in this study be able to used as a basic for selection.
POTENSI EKSTRAK BUAH PARE (Momordica charantia L.) TERHADAP JUMLAH SEL LEYDIG DAN HORMON TESTOSTERON MENCIT (Mus musculus) JANTAN
Authors: Putri Faradillah Hapsari Dwi , Wurlina Wurlina , Tehupuring Benjamin Christoffel
Published: June 2020

The active compound in bitter melon, i.e., flavonoid, is allegedly inhibiting the formation of testoterone by Leydig cell. The aim of the research was investigating the Leydig cell and testoterone level due to the addition of bitter melon extract (Momordica charantia L.). This research was conducted on December 2016 at the Animal Testing Lab of Faculty of Veterinary Medicine Universitas Airlangga, while the production of histopathology preparations was at the pathology laboratory of Universitas Airlangga Surabaya, and the execution of testosterone level test was at Balai Besar Laboratorium Kesehatan Surabaya. The utilized research design was Completely Randomized Design, exploiting 20 male house mice which are divided into four experimental groups of 5 male house mice each. Each group had given the treatments of bitter melon extract of 23mg/kgBB, 35mg/kgBB, 45,5mg/kgBB, while the control group had given the drug solvent of CMC Na 1%, which is conducted by utilizing feeding needles. In the 41st day, the male house mice were being euthanized by cervical dislocation. The Leydig cells could be observed by utilizing 400x microscope zoom, while the testosterone was examined utilizing Enzym Linken Immunosorbent Assay (ELISA). The research result demonstrated that the bitter melon extract (Momordica charantia L.) addition could decrease the Leydig cells and the testosterone level.
HUBUNGAN MORFOMETRI DENGAN PRODUKSI SUSU SAPI PERAH PERANAKAN FRIESIAN HOLSTEIN (PFH)
Authors: Putra Yudhistira Eka , Mulyati Sri , Mumpuni S. Sri
Published: June 2020

Dairy cow’s is one of an animal which produce milk. It capable to supply the high necessary of milk in Indonesia. Many factors can influence milk production. Genetic, age, udder size, weight, lactation period, feed are the important part to decided milk production. The research aimed to study the correlation of morphometry based on chest circumference, body length, height, udder circumference, high of udder toward milk production. Data taken in form of chest circumference, body length, height, udder circumference, high of udder, and milk production. Data have been collated and analysize using regression analysis with SPSS software. The result showed that high of udder have a correlation with milk production. Chest circumference, body length, height, and udder circumference have no showed a correlation with milk production.
PENGUKURAN NILAI KONDISI TERNAK (NKT), CONCEPTION RATE (CR), SERVICE PER CONCEPTION (S/C) PADA SAPI CROSSBREED LIMOUSIN DI KECAMATAN BALONGPANGGANG KABUPATEN GRESIK
Authors: Septian H Fajar , Mustofa Imam , Sektiari L. Bambang
Published: June 2020

This research was conducted to find out the Body Condition Score (BCS, in nine scale) of 30 Limousine crossbred cows in Balongpanggang, Gresik regency at the time of  Artificial Insemination,  21 and 60 days later. Questionnaires obtained to identify the knowledge of farmers and inseminators in estrous detection. Conception Rate (CR) and Service per Conception (S/C) counted based on pregnancy diagnosis through rectal palpation 60 days post insemination. The result showed that the average of BCS in pregnant cows at 60 days post insemination was highest compared to the others. Conception rate and service per conception of the cows were 60% and 1.63 respectively, meanwhile the knowledge of estrous detection of farmers was lower compared to those of inseminator. It could be concluded that implementation of AI technique in Limousine crossbred cows at Balongpanggang, Gresik regency was good enough, but the knowledge of farmers about estrous detection needs to be improved.
PENGARUH EKSTRAK DELIMA MERAH(Punicagranatum L.) TERHADAP PENINGKATAN PERSENTASE KAPASITASI DAN REAKSI AKROSOM SPERMATOZOA TIKUS PUTIH (Rattusnorvegicus)
Authors: Satrio Baktiar Budi , Hariadi Mas’ud , Wahjuni Retno Sri , Utomo Budi , Sudjarwo Sri Agus , Damayanti L Tita
Published: June 2020

The aim of this research is to know the effect of pomegranate (Punicagranatum L.) on the improvement of spermatozoa quality that is increase of percentage of capacitation and acrosome reaction in white rat (Rattusnorvegicus). This research used 20 healthy white rat (Rattusnorvegicus) with average age 2-3 months old, 200-300 g. Research design using completely randomized design (CRD) with four treatments and 5 replications. Rats were randomly divided into 4 treatment groups. Rat in the negative control group not treated just kept alone, solvent control group were given CMC Na 0,5% and is exposed to heat, the treatment groups 1, 2, and 3 induced pomegranate extract standardized to 40% ellagic acid at a dose of 75mg / kg, 150mg / kg and 300mg /kg perorally with doses 1 ml/rat/day gastric for along 14 days and exposed to heat. Data were analyzed using One Way Analysis of Variant (ANOVA) followed by Duncan (p<0,05). The result showed that there was significant effect from therapy on the quality of rats spermatozoa especially to capacitation and acrosome reaction. The treatment from treatment 2 group showed the best result in maintaining the quality of rats spermatozoa with 27.6± 3.04 values for capacitation and 6,80 ± 1,30 for acrosome reactions. Extract of pomegranate (Punicagranatum L.) standardized to 40% ellagic acid has a significant change in improve the quality of spermatozoa, namely an increase in the percentage of capacitation and acrosome reactions in white rats (Rattusnorvegicus) exposed to heat.
PERANAN VITAMIN B12 DALAM PENGENCER SUSU SKIM KUNING TELUR TERHADAP MOTILITAS DAN VIABILITAS SPERMATOZOA DOMBA SAPUDIPOST THAWING
Authors: Arini Mirza Asafaizh , Suprayogi Tri Wahyu , Warsito Sunaryo Hadi , Sardjito Trilas , Susilowati Suherni , Triana Indah Norma
Published: June 2020

The purpose of this study was to prove that the addition of Vitamin B12 in egg yolk skim milk extender can maintain the motility and viability of post thawing Sapudi sheep spermatozoa. This study used sample of Sapudi sheep fresh semen that was collected with artificial vagina, and divided into 3 different treatments. The control treatment or P0, Sapudi sheep semen was diluted in the egg yolk skim milk extender + vitamin C 0,02 mg / ml, treatment group (P1 and P2), Sapudi sheep semen was diluted in the egg yolk skim milk extender+ vitamin B12 5 μg / ml and grouped in P1, Sapudi sheep semen was diluted in the egg yolk skim milk extender + Vitamin C 0.02 mg / ml + Vitamin B12 5μg / ml and grouped in P2. The data analysis was use One Way Analysis of Variant (ANOVA), then to identify the real difference among the treatments continued with Duncan distance test. The result of data analysis showed that P2 treatment was significantly different (p <0.05) with P1 treatment and P0 treatment or control. While P1 treatment was not significantly different (p> 0,05) with P0 treatment or control. The result showed that the addition of vitamin C 0.02 mg / ml with vitamin B12 5 μg / ml combination in egg yolk skim milk extender was the best addition to maintain the motility and viability of the post thawing Sapudi sheep spermatozoa.
PENGARUH PENAMBAHAN EKSTRAK TEH HIJAU (Camellia sinensis) DALAM BAHAN PENGENCER SUSU SKIM TERHADAP KUALITAS SPERMATOZOA DOMBA SAPUDI YANG DISIMPAN PADA SUHU DINGIN
Authors: Vladika Maulinda Agchirilia , Warsito Sunaryo Hadi , Susilowati Suherni , Hernawati Tatik , Kurnijasanti Rochmah , Restiadi Tjuk Imam
Published: June 2020

The purpose of this study was to determine the effect and the best concentrasion of green tea extract in skim milk diluent for motility, viability, and intact plasma membrane Sapudi sheep spermatozoa that was stored 50C. The semen was divided into four groups: skim milk  diluent; 0,05 mg green tea extract in skim milk diluent; 0,10 mg green tea in skim milk diluent and 0,15 mg green tea extract in skim milk diluent. Spermatozoa quality was observed first day until fifth day after diluent. The data obtained was analyzed with the analysis of variance one way ANOVA. The result showed that the percentage of motility, viability and intact plasma membrane spermatozoa at the 5th day after diluent showed a significant differece (p<0,05) between 0,05 mg green tea extract in skim milk diluent; 0,10 mg green tea in skim milk diluent and 0,15 mg green tea extract in skim milk diluent. Motility at the 3th day after diluent respectively 46,67b ± 2,582; 43,33bc ± 4,028; 40,00ab ± 3,162; and 37,50a ± 2,739. Viability  at the 3th day after diluent respectively are 79,50b ± 1,643; 77,67b ± 3,327; 77,00ab ± 3,230; and 73,83a ± 3,061. Intact plasma membrane at the 3th day after diluent respectively are 50,17b ± 2,483; 47,00ab ± 3,899; 46,83ab ± 2,858; and 46,00a ± 3,286. The conclusion of this study was the green tea extract of 0,05 mg in milk skim diluent could increase the percentage of sperm quality until 5th day after diluent.
PENGARUH PENAMBAHAN EKSTRAK TEH HIJAU (Camellia sinensis) DALAM BAHAN PENGENCER SUSU SKIM KUNING TELUR TERHADAP KUALITAS SPERMATOZOA DOMBA SAPUDI YANG DISIMPAN PADA SUHU DINGIN
Authors: Swari Wahyu Retno , Sabdoningrum Emy Koestanti , Wurlina Wurlina , Susilowati Suherni , Kurnijasanti Rochmah , Safitri Erma
Published: June 2020

The purpose of this study was to determinated the effect and the best concentration of greentea extract in skim milk and egg yolk diluent for quality sapudi sheep spermatozoa were measured in motility, viability and intact plasma membrane that was stored on cold temperature. The semen was devided into four groups; skim milk and egg yolk diluent, 0,5% green tea extract in skim milk and egg yolk diluent, 0,1% green tea extract in skim milk and egg yolk diluent, and 0,15% green tea extract in skim milk and egg yolk diluent. Spermatozoa quality was observed day 1, day 2, day 3, day 4 and day 5 after being diluted. The data obtained was analyzed with the Analysis of Variant (ANOVA), followed by multiple range test duncan. The results showed that the highest percentage of motility, viability and intact plasma membrane derived from green tea extract 0,15% on skim milk and egg yolk diluent  for 1 day storage is 86.66b ± 2.58, 92.00b ± 1.78, and 74.16b ± 3.25. The lowest percentage of motility, viability and intact plasma membranes was obtained from the skim milk and egg yolk diluent for 5 days storage is 43.33a ± 6.05, 56.50a ± 3.08 and 28.33a ± 2.80. The conclusion of this study is the addition of 0.15% green tea extract in a skim milk and egg yolk diluent can maintain the quality of sperm for up to 5 days of storage at cold temperatures.
PENGARUH PENAMBAHAN EKSTRAK TEH HIJAU (Camellia sinensis) DALAM BAHAN PENGENCER KUNING TELUR SITRAT TERHADAP KUALITAS SPERMATOZOA DOMBA SAPUDI YANG DISIMPAN PADA SUHU DINGIN
Authors: Khoirunnisa Itrah , Susilowati Suherni , Maslachah Lilik , Suprayogi Tri Wahyu , Kurnijasanti Rochmah , Ratnani Hermin
Published: June 2020

The purpose of this research was determine the effect and the best concentration of greentea extract in egg yolk citrate diluent for quality Sapudi sheep spermatozoa were measured in motility, viability and intact plasma membrane that was stored on cold temperature. The semen was devided into four groups : egg yolk citrate diluent, 0,05 % green tea extract in egg yolk citrate diluent, 0,1 % green tea extract in egg yolk citrate diluent and 0,15 % green tea extract in egg yolk citrate diluent. Spermatozoa quality was observed day 1, day 2, day 3, day 4 and day 5 after diluent. The data obtained was analyzed with the analysis of variance (ANOVA), followed by multiple range test duncan. The result showed that the higest precentage of motility, viability and intact plasma membran were obtained from 0,05 % green tea extract in egg yolk citrate diluent for 1 day of storage which were 85.83 ± 3.76, 91.16 ± 2.13 and 66.83 ± 6.49. The lowest precentage of motility, viability and intact plasma membrane were obtained from egg yolk citrate diluent for 5 day of storage which were 26.66 ± 6.05, 49.16 ± 6.43 and 26.66 ± 4.32. Conclution of this research was the addition of 0,05 % green tea extract can be used as a diluent could maintain the precentage of spermatozoa quality until 5 days after diluent.
PENGARUH PENGENCER KUNING TELUR AYAM DENGAN AIR KELAPA MUDA TERHADAP INTEGRITAS MEMBRAN PLASMA DAN ABNORMALITAS SPERMATOZOA DOMBA SAPUDI PENGARUH PENGENCER KUNING TELUR AYAM DENGAN AIR KELAPA MUDA TERHADAP INTEGRITAS MEMBRAN PLASMA DAN ABNORMALITAS SPERMATOZOA DOMBA SAPUDI
Authors: Cesaria Mayrena Ayu , Estoepangestie A.T.Soelih , Susilowati Suherni , Hernawati Tatik , Madyawati Sri Pantja , Triana Indah Norma
Published: June 2020

This study aimed to determine the influence of mixture diluter of chicken egg yolk and young coconut water on the integrity of plasma membrane and abnormalities of Sapudi sheep spermatozoa. The experimental design used in this study was a complete random method. The data was analysed using ANOVA, and if there is a significant difference then proceed with Duncan’s Multiple Range Test ( DMRT ) at significance level of 5%. Based on the result of the research, the influence of mixture diluter of chicken egg yolk and young coconut water was not significantly different ( p > 0,05 ) on the intact plasma membrane of spermatozoa on the first day until the fourth day. Interaction between treatment and storage duration had no effect on spermatozoa abnormality on the first day, second day and the fourth day. It can be concluded that mixture diluter of chicken egg yolk and young coconut water had a good effect on the plasma membrane and spermatozoa abnormalities up to the third day.
PENGARUH pH LENDIR MUKOSA VAGINA SAAT BIRAHI TERHADAP PERSENTASE KEBUNTINGAN (Conception Rate) PADA SAPI PERAH DI KUD TANI WILIS KABUPATEN TULUNGAGUNG DAN KSU TUNAS SETIA BARU KABUPATEN PASURUAN
Authors: Rizki Andaru , Srianto Pudji , Suprihati Endang , Sardjito Trilas , Ismudiono Ismudiono , Al Arif Mohammad Anam
Published: June 2020

The purpose of this research is to determine the effect of mucos acidity of vaginal mucous when estrus towards conception rate at KUD Tani Wilis Tulungagung District and KSU Tunas Setia Baru Pasuruan District. Measured samples were taken from 30 dairy cattles in each KUD. Mucos acidity of vaginal mucous were obtained by using a digital acidity meter before insemination was done on dairy cattle. After measuring the acidity from mucosal vagina mucous, artificial insemination has done on estrus dairy cattle. On the 60th day after insemination, rectal palpation was done to check the conception rate. Research result from 30 dairy cattle on each KUD showed that mucos acidity of vaginal mucous has signifficant effect towards conception rate. Mucos acidity of vaginal mucous range 6.5-8.5 with the highest range of conception rate in KSU Tunas Setia Baru Pasuruan District 7.5-7.7 and in KUD Tani Wilis Tulungagung District with the highest range of conception range 7.2-7.4 and 7.5-7.7.
HUBUNGAN ANTARA UMUR DENGAN CALVING INTERVAL, DAYS OPEN, DAN SERVICE PER CONSEPTION SAPIFRIESIAN HOLSTEIN (FH)
Authors: Ananda Hanifah Muslimah , Wurlina Wurlina , Hidajati Nove , Hariadi Mas’ud , Samik Abdul , Restiadi Tjuk Imam
Published: June 2020

The purpose of this research was to know the relationship between age with calving inteval (CI), days open (DO), and service per conception (S/C) in Friesian Holstein dairy cattle (FH). The research was started on Desember 2017 to January 2018 in PT. Greenfields Indonesia partnerships, KecamatanWagir Kabupaten Malang. The materials of the search used were 100 heads of dairy cattle which had at least two times of parturition. The method used in this research was a survey and data collection. The data were obtained from the records of reproduction. Data analysis was multiple linear regression using SPSS software 21.0 version. The results showed that the values of CI (434,9±58,9 days, 449,4±66,2 days, and 431,8±59,2 days), DO (218,9±58,7 days, 218,9±58,7 days, dan 217,6±54,1 days), dan S/C (3,2±1,8 times, 4,3±1,9 times, 2,6±1,1 times) for the ages of 4, 5, 6. The relationship between age with CI and DO were not significant (P>0,05), but the relationship between age with S/C was significant (P<0,05). The value of S/C increased on dairy cattle at age of 4 to 5 years and started to decreased at the age of 6 years.
Effect of α-tocopherol supplementation in diluents on the motility, viability and plasma membrane integrity of Simmental bull spermatozoa after cooling
Authors: Azura Sarah , Ratnani Hermin , Susilowati Suherni , Hariadi Mas'ud , Samik Abdul , Soepranianondo Koesnoto
Published: June 2020

Semen storage in cold temperatures might cause an increase in reactive oxygen species (ROS) production. This condition resulted in spermatozoa damage and quality decrease. This study was conducted to investigate the effects of α-tocopherol supplementation in diluents on the motility, viability, and plasma membrane integrity of Simmental bull spermatozoa after cooling. Semen samples were diluted in skim milk egg yolk supplemented with 0, 0.5, 1.0, and 1.5 mM α-tocopherol respectively for control, Tl, T2, and T3. Spermatozoa were evaluated for their motility, viability, and membrane integrity in cooling temperature (5°C). The daily evaluation showed that 1.5 mM α-tocopherol was the best in maintaining motility, viability, and plasma membrane integrity, while 1.0 mM α-tocopherol was only good for maintaining viability. Therefore, it can be concluded that α-tocopherol at the concentration of 1.5 mM was an efficient antioxidant supplement for Simmental cattle semen in skim milk egg yolk diluent.
The Influence of Transcultural Nursing Based on Health Education on Knowledge, Attitude, Mother Actions and Family Support among Breastfeeding Mother
Authors: Naragale Yulia Meiliany , Kusumaningrum Tiyas , Pradanie Retnayu
Published: June 2020

Introduction: Providing additional food other than breast milk before 2-year-old babies in Kupang City are babies given "regal" biscuits, "milcuit" biscuits, and "banana" s because the baby does not feel hungry. Providing "regal" biscuits, "milna" and "banana" biscuits as a complement to give breastfeeding before the age of 2 years because parents believe these biscuits can eliminate hunger for the baby. The purpose of this study was to explain the effect of Transcultural Nursing-based health education on knowledge, attitudes, actions of mothers, and family support in breastfeeding.Methods: The design of this study was a Pre-Experimental study using quantitative data. Samples of 30 breastfeeding mothers who visited the Bakunase Public Health Center in Kupang City of East Nusa Tenggara and were obtained using the total sampling technique. Data obtained from the knowledge questionnaire, attitude questionnaire, action questionnaire, and social and family support questionnaire were then analyzed using the Wilcoxon statistical test.Results: The results showed that there was an influence of transcultural nursing-based health education on knowledge, attitudes, mother actions, social and family support in breastfeeding with a significant value of p=0.000.Conclusion: Transcultural nursing-based health education influences maternal knowledge, maternal attitudes, and family support for mothers in breastfeeding. Subsequent researchers can then carry out transcultural nursing-based health education research on different targets. They can analyze factors related to knowledge, attitudes, actions, and family support for nursing mothers.
The Effect of a Combination of Oxytocin Massage and Music Therapy on Breast Milk Production and Breastfeeding Self Efficacy in Primipara Post Partum Mothers
Authors: Wulandari Sri , Triharini Mira , Wahyuni Sylvia Dwi
Published: June 2020

ABSTRACTIntroduction: exclusive breastfeeding. Factors that affect mothers not to give breast milk to babies, including the condition of mothers who are stressed, lack of confidence can cause the production of breast milk not smooth. The aim of this study was to study the combination of oxytocin therapy and music therapy in breast milk production and breastfeeding self-efficacy in postpartum mothers.Method: The study design used Quasy Experiment with a pre-posttest design. The population of mothers after childbirth who worked in the Kalijudan Health Center area, Surabaya. The sample used was 80 respondents, divided into 2 groups, 40 intervention groups and 40 control groups with purposive sampling. The independent variable is a combination of oxytocin therapy and music therapy. The dependent variable is breast milk production and breastfeeding self efficacy. The instrument used was a questionnaire breastfeeding production and breastfeeding self efficacy was validated and reliably. The research data were analyzed by the Wilcoxon Signed Rank Test and the Mann Withney test.Result: the results showed that there was a combination of oxytocin therapy and music therapy in breast milk production (p = 0,000) and breastfeeding self efficacy (p = 0,000) in the treatment group. There was a significant difference between the control and treatment groups in breast milk production (p = 0,000) and breastfeeding self efficacy (p = 0,000). Conclussion: the combined effect of oxytocin massage and music therapy effectively increases breast milk production and the efficacy of caring for it. Keyword: Oxytocin Massage, music therapy, ASI production, Breastfeeding Self Efficacy.
Evolution de la production laitière au cours de la lactation : modèle de prédiction chez la vache laitière
Authors: COULON J.B. , PÉROCHON L.
Published: June 2020

L’objectif de cette étude a été de construire un modèle empirique de prédiction de l’évolution de la production laitière au cours de la lactation chez des vaches correctement alimentées et indemnes de troubles sanitaires majeurs. Il a été élaboré à partir d’un jeu de 877 lactations provenant de 5 troupeaux expérimentaux de l’INRA, représentant une large gamme de situations (niveau de production, période de vêlage, âge…) et permettant de disposer d’échantillons distincts pour la construction et la validation du modèle. Le modèle final proposé intègre les effets du stade de lactation, du stade de gestation et de la saison. Les variables d’entrée nécessaires sont la date de vêlage, la parité, la date d’insémination fécondante et le potentiel de production. Ce dernier est estimé à partir d’une valeur de production mesurée entre les semaines 2 à 8 de lactation. Le modèle final permet un très bon ajustement des courbes de production individuelles, même lorsqu’elles présentent des formes inhabituelles. Sa qualité de prédiction à l’échelle individuelle est limitée : 29% des lactations de l’échantillon de validation présentent un écart moyen au modèle supérieur à 2 kg/j. En revanche, pour des groupes de vaches la prédiction devient excellente : sur des lots de 20 vaches, l’écart moyen au modèle ne dépasse 1 kg/j que dans 2 % des cas. Ce modèle est donc un outil efficace de prédiction d’une référence de production à l’échelle d’un troupeau. Les données nécessaires à son utilisation pratique sont précisées en annexe.
Les techniques de base de la génétique moléculaire
Authors: RIQUET J. , PITEL F.
Published: June 2020

Les techniques les plus couramment utilisées en génétique moléculaire font l’objet de cet article. La première partie présente les outils de base, fondés sur des principes de physique, de chimie ou de biologie. Différentes combinaisons de ces outils sont ensuite développées pour aborder les techniques utilisées dans différents domaines de la biologie moléculaire : fragmentation, séparation, repérage, multiplication et lecture de l’ADN. L’accent est mis sur la diversité des techniques pouvant être mises en oeuvre et sur la vitesse d’évolution de ces techniques.
Introduction à la cartographie des génomes complexes
Authors: CRIBIU E.P. , SCHIBLER L.
Published: June 2020

Les technologies mises en place dans le cadre des projets sur les génomes humain et murin ont stimulé le développement de la cartographie des espèces d’intérêt agronomique. Des cartes ont été construites pour les ruminants, le porc, le cheval et le poulet, permettant de localiser des gènes d’intérêt agronomique ou des QTL (quantitative trait loci) chez ces espèces. Ces travaux de cartographie génétique, cytogénétique et physique devraient se révéler être des outils indispensables pour le clonage positionnel de ces gènes ou QTL.
Cartographie fine d’un gène : bilan INRA et autres résultats marquants
Authors: MULSANT P.
Published: June 2020

Plusieurs programmes de cartographie fine et d’identification de gènes majeurs sont actuellement en cours à l’INRA, et certains ont abouti (caséine, RN) ou devraient déboucher rapidement sur l’identification de la mutation causale (PIS, Polled, Booroola). Les caractéristiques des principaux programmes sont présentées, ainsi que les raisons de leur progression. Les possibilités d’amélioration à court terme sont discutées.
Exemple d’approche fonctionnelle : la fonction ovarienne dans l’espèce porcine
Authors: TOSSER-KLOPP G. , CLOUSCARD-MARTINATO C. , BENNE F. , BONNET A. , CABAU C. , RALLIERES J. , HATEY F.
Published: June 2020

La recherche de gènes ovariens hormono-régulés a été entreprise par l’hybridation différentielle de 40 000 clones d’ADNc d’une génothèque de cellules de granulosa porcine en culture et par des expériences de Differential Display. Environ 150 clones exprimés différentiellement, selon qu’il s’agit de cellules traitées par la FSH ou de cellules témoins, ont été séquencés et 59 d’entre eux ont été identifiés. La régulation de ces gènes a été étudiée in vitro et in vivo par des techniques de Northern blot, RT-PCR comparative et hybridation sur coupes d’ovaires. Environ cinquante gènes ont été localisés sur le génome porcin. Ces données fonctionnelles et génétiques permettent de considérer certains de ces gènes comme des candidats intéressants pour la fonction de reproduction.
COVID-19: Probing the Possibility of Transmission in Animal Kingdom
Authors: Kazi Tawsif Ahmed , Mitra Suranjita , Mukhopadhyay Bidhan Chandra , Bhattacharya Rajarshi , Mandal Sukhendu , Biswas Swadesh Ranjan
Published: June 2020

The recent 2019-nCoV outbreak, spreading infection around the globe is jeopardizing the public health and global economy. The virus was reported to have emerged from an animal market in Wuhan, China at the end of 2019 and presumed to have originated from bats and eventually transmitted in humans. The entry of the virus into human cells is triggered by a series of molecular events initiated with the binding of a receptor-binding domain of viral spike protein to human Ace2 cell surface receptor. Based on the comparative sequence analysis of the well-known binding hotspots of human Ace2, cross-interacting potential of 2019-nCoV was predicted, which suggests Ace2 of wild animals like tiger, bear, orangutan, etc.; aquatic mammals like whale and dolphins; and domestic animals like cat, horse, goat, sheep, dog etc. as potential target. However, the recognition of Ace2 of bats, rats and mice by the 2019-nCoV spike protein remains under question. The study indicates that 2019-nCoV might have broad host range and may thus intensify the gravity of 2019-nCoV outbreak
Design and Simulation of Voltage Amplidyne System using Robust Control Technique
Authors: Jibril Mustefa , Tadese Messay , Alemayehu Eliyas
Published: June 2020

In this paper, modelling designing and simulation of a simple voltage amplidyne system is done using robust control theory. In order to increase the performance of the voltage amplidyne system with H ∞ optimal control synthesis and H ∞ optimal control synthesis via ∞-iteration controllers are used. The open loop response of the voltage amplidyne system shows that the system can amplify the input 7 times. Comparison of the voltage amplidyne system with H ∞ optimal control synthesis and H ∞ optimal control synthesis via ∞-iteration controllers to track a desired step input have been done. Finally, the comparative simulation results prove the effectiveness of the proposed voltage amplidyne system with H ∞ optimal control synthesis controller in improving the percentage overshoot and the settling time.
Genomic Variations in ORF3a and ORF1ab Genes of SARS-nCoV2 Strain from Southern Pakistan
Authors: Saif Rashid , Ejaz Aniqa , Mahmood Tania , Zia Saeeda , Qureshi Abdul Rasheed
Published: June 2020

Emergence of COVID-19 pandemic has resulted in 8,578,283 total cases and 456,286 deaths worldwide as of June 19, 2020. We previously analysed genomic variants in two Northern Pakistani SARS-nCoV2 strains against USA and Chinese strains as reference, and hypothesized the putative role of observed variants in low severity of COVID-19 in Pakistan. Due to high variation rate in this virus, we further analysed the whole genome of Southern Pakistani SARS-nCoV2 MT500122 strain (Karachi-Pak) vs NC_045512 (Wuhan1-China) and observed 4 variants (3=SNPs,1=del). Three of variants at g.1604 (del ND447N), SNPs at g.1912 (p.=), g.10582 (p.=) and g.26022 (p.=) in ORF1ab and ORF3a genes respectively. ORF1ab encodes 16 non-structural polyproteins (nsps1-16) and plays role in viral replication. The codon change deletion in its sequence (as observed in MT500122) might have caused conformational alterations particularly in nsp2&5 structures which may obstruct its effectiveness. ORF3a is unique to SARS-nCoV2 and located in-between envelope and spike genes, which assist viral entry into the host cell by interacting with S gene. Alteration in its sequence might have hampered the activation of S gene and affect its binding capacity to host cell ACE2 and NRP1 receptors, which may greatly weaken its pathogenicity in its different strains and hence may vary severity of COVID-19. Nevertheless, intensive data and conclusive wet lab experiments are needed for validating this postulated hypothesis. Moreover, these variants have modifier to silent impact on further 9 genes e.g. M, N, S, E, ORFs 6, 7a, 7b, 8 and 10 as well. Advancements in understanding the role of these Pakistani SARS-nCoV2 genomic variations will be helpful in developing indigenous vaccines, diagnostic kits and drug development.
Survival and Disinfection of Sars-Cov-2 in Environment and Contaminated Surface
Authors: Al-Gheethi Adel , Noman Efaq , Al-Maqtari Qais , Almoheer Reyad , Mohamed Radin , Hamdan Rafidah
Published: June 2020

The detection of SARS-Cov-2 in the sewage and water resources has increased the awareness among the people about the possibility survival of SARS-Cov-2 in the environment and the potential to transmit into the human through food chain or water resources. Moreover, the surface contaminated by the virus need to be disinfected frequently by using an effective disinfectant, the current chapter discussed the efficiency of the most traditional treatment process of the sewage and wastewater, and their role in the elimination of the virus as well as the sterility assurance level concept. Moreover, the chemical disinfectant used currently and their temporary efficiency has been reviewed.