bluuebunny/crossref_metadata_2025 huggingface dataset:
DYNAMIC MODEL WITH A NEW FORMULATION OF CORIOLIS/CENTRIFUGAL MATRIX FOR ROBOT MANIPULATORS
Authors: Truc Le Ngoc , Quyen Nguyen Van , Quang Nguyen Phung
Published: March 2020

The paper presents a complete generalized procedure based on the Euler-Lagrange equations to build the matrix form of dynamic equations, called dynamic model, for robot manipulators. In addition, a new formulation of the Coriolis/centrifugal matrix is proposed. The link linear and angular velocities are formulated explicitly. Therefore, the translational and rotational Jacobian matrices can be derived straightforward from definition, which makes the calculation of the generalized inertia matrix more convenient. By using Kronecker product, a new Coriolis/centrifugal matrix formulation is set up directly in matrix-based manner and guarantees the skew symmetry property of robot dynamic equations. This important property is usually exploited for developing many control methodologies. The validation of the proposal formulation is confirmed through the symbolic solution and simulation of a typical robot manipulator.
Gente, cambio e innovación: Condiciones para la perdurabilidad empresarial
Authors: Rivera Rodriguez Hugo Alberto , Gonzalez Rodríguez Wilson Omar
Published: March 2020

Este articulo documenta los factores que inciden en la perdurabilidad empresarial. Corresponde a un estudio exploratorio con un enfoque cualitativo en el procesamiento de la información. Resultados: Se encontró que las condiciones que contribuyen a la perdurabilidad de las empresas estudiadas son, la gente, como factor en el logro de los objetivos; el cambio como motor de adaptación; y la innovación en la búsqueda de oportunidades de negocio.
ENZYMATIC MODIFICATION OF WHEAT RICE
Authors: Buzhylov M. , Kaprelyants L. , Pozhitkova L. , Kishenya A. , Soboleva A.
Published: March 2020

The article presents conceptual approaches to solving technological and technical problems in the creation of functional foods. General approaches are proposed to change existing technologies to improve the efficiency of integrated raw material processing and to increase the production of high-quality foods and food ingredients with antioxidant properties. Cereal crops are the richest source of functional ingredients and a major component of human nutrition. It is proved that most of the nutrients are in the products of its processing. For the first time, polyphenols from cereal raw materials were obtained by biotechnological means. The feasibility of pretreatment of raw materials with amylolytic and proteolytic enzymes for purification and cleavage of polysaccharide matrix has been established. Based on the regularities of enzymatic hydrolysis of polysaccharides, we used the processing of wheat bran with multifunctional drug Viscozyme L with hemicellulase, cellulase, pectinesterase and feruloesterase activities, which resulted in a high effect of degradation of certain covalent cells, ferulic acid from 40.99 to 2507.9 mcg / g. It is determined that this method of obtaining the target components allows to preserve their native structure, especially the supramolecular structure, which determines their physiological effect. The influence of plant polyphenols on the cultivation of probiotic microorganisms is characterized. the comparative characterization of the prebiotic properties of the polyphenols obtained from wheat bran and the concentrate of the polyphenols from the grape buds "ENOANT" are substantiated. The possibility of increasing the proportion of free polyphenols by fermentation of wheat bran is shown. It is established that the extract of polyphenols from wheat bran can be used for its purpose as an effective antioxidant, which does not have a negative effect on the state of the basic physiological systems of the body.
SCIENTIFIC SUBSTANTIATION OF TECHNOLOGY OF PASTA BASED ON FRESHWATER MUSSELS ENRICHED WITH SELENIUM
Authors: Holovko Mykola , Holovko Tetyana , Prymenko Vladyslav , Helikh Anna , Zherebkin M.
Published: March 2020

The possibility of using dietary selenium-protein additive "Neoselen" for enrichment of pastes based on mussels with organic selenium has been investigated. The formulation of the pastes involved the use of the soft body of the freshwater mmussels of the genus Anodonta. The influence of the introduction of different percentage of the additive "Neoselen" on the organoleptic properties of the developed pastes based on freshwater mussels was investigated. It is found that the addition of additive "Neoselen" even more than 5% does not impair the taste and color of the developed pastes. Moisture content, moisture retention, fat-holding capacity and active acidity of the developed pastes were investigated. The moisture-holding and fat-holding capacity of the developed paste is determined. The difference in the consistency of pastes based on freshwater mussels with different percentage of unpowered additives was investigated by determining the values of plastic viscosity and modulus of elasticity. The regularities of the influence of the additive "Neoselen" on the general chemical and mineral composition of pastes based on freshwater mussels have been established. It is established that the addition of 1%, 3% or 5% in the paste of additive "Neoselen" can enrich it by 7.8, 23.4 and 39 mkg of selenium, respectively. Studies have shown that in the composition of both the control and experimental samples of pastes in the amount of protein, minerals and water content. With the increase of the content of the additive "Neoselen" in the formulation of pastes, the content of protein and minerals increases. The complex of qualitative characteristics of the developed pastes is investigated. The regularities of the influence of the component composition on the organoleptic and physicochemical and functional-technological characteristics of the minced systems based on freshwater mussels and the additive "Neoselen" have been established. It is established that the optimal doses of the use of additive "Neoselen" in the formulation of pastes ranges from 3-5%.
STABILITY OF THE STRAINS OF BASIDIOMYCETES DURING STORAGE IN THE COLLECTION
Authors: Miros S. , Koocherov V. , Bilokon S. , Sechnyak A.
Published: March 2020

Basidial macromycetes may be a material for the development of new biotechnologies, medical preparations, components of dietary nutrition. Therefore, it is necessary for the highest level of quality for maintenance and identification of mushroom strains in the collection. An important parameter, in this case, is a stability of isolated and described collection strains of basidiomycetes. Stability is one of the key issues of long-term preservation of pure culture collections. For the collection of medicinal basidiomycetes of ONU I.I. Mechnikov, which preserves by the method of periodic reseeding of colonies the strain stability had not been studied yet. The goal of this research is to study the stability of this collection by a growth rate of mushroom colonies and electrophoretic spectra of carboxylesterases after different times of storage of cultures on malt agar. In this research the strains of three age categories (1, 2 and 3 years) of storage on malt-agar medium at temperature 4 ° С for were tested. The radial growth rate of their vegetative mycelium and the spectra of multiple molecular forms of carboxylesterases by the method of vertical electrophoresis in 7% of polyacrylamide gel were investigated. It was established that the stability of the radial growth rate of A. auricula-judae, F. velutipes, G. lucidum the vegetative mycelium after different storage periods is high according to the values of variation coefficients. At the same time, the expression of molecular forms of carboxylesterase showed sufficient variability. Partially conservative molecular forms were detected in some age groups of strains, as well as for individual strains. Thus the growth rate of colonies is a stable indicator and the molecular forms of carboxylesterases of different ages strains are variable.
Reflections on General Practice Training in China: Innovative and Implementable ideas from the Guangdong General Practice Symposium
Authors: Liu Xiaoyun , Xu Daniel , Zhou Fengli , Zhang Kouqing
Published: March 2020

This article was migrated. The article was not marked as recommended. Introduction:The concept of general practice in China was first introduced in 1980s in line with several years of healthcare reform. In the last 30 years of general practice development in Australia, the gatekeeping role of general practice has been slowly developed and formulated into health policy and has made steady progress with some remarkable success in certain parts of China. In Australia, general practice education and training were initiated at the time of the founding of the Royal Australian College of General Practitioners in 1964 and a relatively mature system of general practice education and training has been established. To establish a more relevant and effective training model of general practitioners in China by reference to Australian model will be an excellent starting point to introduce into the general practice education, training, clinical services and heath policy strategic plans in China. Methods: Feedbacks from general practice trainees at the Guangdong General Practice Forum collected via an online portal after the lectures and were subsequently analysed. Results: The feedback survey contained the evaluation questions regarding the GP's response to the academic contents, organization and administration of the forum. The GP trainers and trainees showed very high satisfaction (100%). Twenty-one (95.45%) of the GP trainees agree that the forum is very useful in clinical practice while one (4.55%) GP trainee disagreed slightly that the forum is ordinary. There were four implementable suggestions obtained from the feedback evaluation during the forum. Conclusions: More regular, frequent and consistent GP training forums with experienced overseas GP trainers and medical educators will be needed to improve GP education and training to accelerate the general practice education and training development in China.
Including widespread geometry schemas into Linked Data-based BIM applied to built heritage
Authors: Bonduel Mathias , Wagner Anna , Pauwels Pieter , Vergauwen Maarten , Klein Ralf
Published: March 2020

A reliable data exchange – often including geometry-related data – between stakeholders is crucial in construction projects. In this regard, data exchange frameworks built on Linked Data principles are very promising for combining disparate data sets. However, existing proposals to combine geometry and Linked Data either demand dedicated applications or support only a limited number of common geometry schemas. If any existing geometry schema could be used in a Linked Data context, error-prone geometry conversions are avoided and stakeholders do not need to invest in new geometry engines. In this paper, the applicability of Resource Description Framework (RDF) literals for including a wide variety of existing geometry schemas is studied and applied in a built heritage context. The uniform linking pattern and related terminology of the Ontology for Managing Geometry are used to implement this approach. Subsequently, the File Ontology for Geometry formats and Geometry Metadata Ontology are developed to ease the reuse of linked geometry descriptions. The effectiveness of the entire data structure is demonstrated in a built heritage case study project. The receiving party is able to create successfully a coordinated view – using a demo web application – on shared, but disparate, RDF data sets containing geometry descriptions.
Implications of different road pricing schemes in urban areas: a case study for Budapest
Authors: Zefreh Mohammad Maghrour , Esztergar-Kiss Domokos , Torok Adam
Published: March 2020

Road pricing is an efficient instrument to regulate use of roads. In Budapest, Hungary, only cordon pricing has been investigated in detail. This paper reports on a study of the implications of applying different road pricing schemes in the city using a macroscopic traffic model. Firstly, the functional relationship between pricing schemes and road transport demand was investigated. Secondly, the implications of applying road pricing schemes on travel demand, travel time, delay time, average speed and carbon dioxide emissions of road users inside and outside of the pricing area, as well as revenues generated by applying the schemes, were studied. The results revealed that a time-based system showed the best performance inside the pricing area. However, it generated heavy congestion on non-priced roads bordering the pricing area, which would increase travel times, delay times and carbon dioxide emissions and would decrease average travel speeds on the entire network. The cordon-based system was the least effective pricing system for most of the studied parameters, while the distance-based system showed good performance both inside and outside the pricing area. Regarding the revenues generated, variable-toll systems (time-based and distance-based systems) showed the potential to generate higher revenues than the fixed-toll system (cordon-based system).
Comparación morfométrica de espermatozoides humanos y de animales domésticos, teñidos con la coloración árbol de navidad
Authors: Lizarazo Quintero Aura Yaneth
Published: March 2020

En el análisis forense de muestras recuperadas de delitos sexuales el semen es el fluido que evidencia la existencia del hecho punible, debido a que uno de sus componentes son los espermatozoides, células que son específicas, se conservan por mayor tiempo, contienen ADN y en un eyaculado humano normal se encuentran de 200 a 400 millones de estas células (1). Razón por lo cual son una herramienta importante en la administración de justicia. Aunque no es frecuente encontrar delitos sexuales asociados con animales, es importante diferenciar las características morfológicas entre el espermatozoide humano y el de otro tipo, de animales, que se relacionan en la vida cotidiana con el ser humano. El estudio pretendió determinar diferencias entre los espermatozoides humanos y animales por medio del método de coloración árbol de navidad y observación microscópica. Se recolectó semen humano y los eyaculados de animales domésticos como: toro, hámster, caballo, perro, cerdo y gato. Luego se realizaron extendidos, los cuales fueron teñidos y analizados en microscopio óptico, encontrando diferencias significativas en los espermatozoides de las especies estudiadas dentro de las que se destacaron: forma, tamaño y color, las cuales permitieron individualizar características propias de cada especie. Se visualizó que el espermatozoide con mayor similitud al de humano es el del perro. La diferenciación de espermatozoides humanos con animales es relevante en la resolución de casos forenses. El estudio morfométrico reveló diferencias evidentes en los espermatozoides analizados, lo cual será una herramienta importante en el momento de su hallazgo en una muestra determinada.
Validación del método de detección de antígeno prostático específico (psa), por inmunocromatografía (Orgenics) aplicado a líquido seminal presente en manchas secas y escobillones
Authors: Cifuentes Sandra Liliana
Published: March 2020

El antígeno prostático específico (PSA) es generalmente usado para detectar y monitorear cuantitativamente el desarrollo de cáncer de próstata por niveles en suero de esta proteína, esta también es encontrada en altas concentraciones en semen. Diversos métodos reproducibles, sensibles y simples han sido desarrollados para el análisis de la presencia de PSA incluyendo técnicas inmunocromatográficas. Los procedimientos más comunes para la detección forense de semen se han enfocado en la identificación de espermatozoides, actividad de la fosfatasa ácida y métodos inmunoelectroforéticos. Aunque estos métodos han sido utilizados por muchos años, existen algunos problemas asociados con cada método. El objetivo de esta investigación fue validar la prueba inmunocromatográfica para la detección de PSA en muestras forenses, para ello se realizaron diferentes diluciones de un patrón de semen con una concentración de PSA de 100.000ng/ml, encontrándose un límite de detección de 10 ng/ml. En manchas secas y escobillones, se presentaron resultados positivos a partir de concentraciones de 500 ng/ml. De igual manera se evaluó el comportamiento de la prueba ante interferentes, tales como sangre, orina masculina y femenina, leche materna, secreciones vaginales, materia fecal y saliva. Mostrando resultados positivos para sangre y orina de un individuo con cáncer de próstata se reporta una sensibilidad de 91.6% y una especificidad de 100%. Los resultados sugieren que este test puede ser usado como presuntivo para determinar la presencia de semen en muestras forenses.
Procesos, procedimientos, dispositivos y técnicas de administración del cuerpo desaparecido
Authors: Muñoz Marín César Augusto
Published: March 2020

Introducción: En este artículo se explora, la forma cómo los procesos de búsqueda e identificación de personas desaparecidas se han convertido en procedimientos técnicos, y de qué manera se han implementado. Metodología: Se realiza la sistematización de algunas de las herramientas de búsqueda e identificación creadas por las entidades encargadas de las políticas públicas sobre desaparición forzada y se hace una etnografía a la morgue y el laboratorio del Instituto Nacional de Medicina Legal en Bogotá. Resultados: Se demuestra la importancia que tiene el diálogo entre las ciencias sociales y las ciencias forenses en la reconstrucción histórica de lo que ha sido la guerra en Colombia. Conclusión: El diálogo entre cientistas sociales y científicos forenses, permite ampliar el concepto de identificación, más allá de la suma de unos procedimientos técnicos que dan como resultado la identidad de una persona, aportando en la construcción de nuevas categorías de representación de la violencia.
Dynamic Beveridge Curve Accounting
Authors: Ahn Hie Joo , Crane Leland D.
Published: March 2020

We develop a dynamic decomposition of the empirical Beveridge curve, i.e., the level of vacancies conditional on unemployment. Using a standard model, we show that three factors can shift the Beveridge curve: reduced-form matching efficiency, changes in the job separation rate, and out-of-steady-state dynamics. We find that the shift in the Beveridge curve during and after the Great Recession was due to all three factors, and each factor taken separately had a large effect. Comparing the pre-2010 period to the post-2010 period, a fall in matching efficiency and out-of-steady-state dynamics both pushed the curve upward, while the changes in the separations rate pushed the curve downward. The net effect was the observed upward shift in vacancies given unemployment. In previous recessions changes in matching efficiency were relatively unimportant, while dynamics and the separations rate had more impact. Thus, the unusual feature of the Great Recession was the deterioration in matching efficiency, while separations and dynamics have played significant, partially offsetting roles in most downturns. The importance of these latter two margins contrasts with much of the literature, which abstracts from one or both of them. We show that these factors affect the slope of the empirical Beveridge curve, an important quantity in recent welfare analyses estimating the natural rate of unemployment.
Nitriding of steels of various structural classes manufactured by laser additive technologies
Authors: Tsvetkova E. V. , Bazaleeva K. O. , Chekin I. S. , Klimova-Korsmik O. G. , Zhidkov A. S.
Published: March 2020

The authors have conducted a comparative analysis of diffusion layers of steels of various structural classes manufactured by complex technology including laser remelting of powder material and plasma nitriding. Parameters of diffusion layers of bainitic steel (Fe – 0.09 % C – 1 % Cr – 2 % Ni – 1 % Mo – 1 % Cu) and martensitic steel (Fe – 0.25 % C – 13 % Cr – 2 % Ni) manufactured by direct laser deposition (DLD) and austenitic steel (Fe – 0.03 % C – 17 % Cr – – 14 % Ni – 3 % Mo) manufactured by selective laser melting (SLM) were investigated. During plasma nitriding at 540 °C for 24 h of martensitic and austenitic steels, diffusion layer of 140 – 160 μm was formed, additionally maximum microhardness of surface layer was 800 HV0.1 and 1050 HV0.1 and it is almost constant on thickness of 100 μm. Diffusing layer of bainitic steel is 900 μm and its microhardness monotonously decreases from the surface. Reinforcing phases of nitrided layer were determined by X-ray analysis: γ′ (Fe4N) is fixed in the bainitic steel, γ′ and CrN are fixed in martensitic and austenitic steels. Moreover on the surface of austenitic steel solid nitrided layer is formed. The influence of heat treatment after laser remelting of powder material was also studied. It was determined, that despite decreasing of crystal structure defects after heat treatment, the thickness of nitrided layer changes slightly. Also the authors have investigated the influence of porosity of austenitic steel on the thickness of nitrided layer. It was shown, that porosity of 0.5 – 2.0 % doesn’t result in changing of diffusion layer’s thickness.
On the movement of briquetted mass in extruder. Exact solutions
Authors: Bizhanov A. M. , Podgorodetskii G. S.
Published: March 2020

The increased interest in cold agglomeration in recent years has been largely due to the successful experience in operating briquette lines based on stiff vacuum extrusion (SVE). The high performance of SVE extruders and the satisfactory metallurgical properties of extrusion briquettes obtained this way (brex) make it possible to consider this cold agglomeration technology as promising. SVE extruders allow efficient briquetting of the materials with moisture contents values in the range of 12 – 16 % and compacting pressure of 3.5 – 4.5 MPa, leading to the possibility of achieving high values of mechanical strength of raw briquettes and eliminates the need for drying briquetted charge and heat treatment of the green briquettes. The growing scale of practical use of extruders in the steel industry necessitated the development of simple and effective methods for determining their operating parameters. The briquetted mass is a moistened plastic mass, driven by the blades of a rotating auger and squeezed out further through the holes in the extruder die in the form of elongated briquettes, repeating in cross section the shape of the hole. In application to the optimization problems of extrusion briquette technology, the exact solution of the Navier–Stokes equations for a viscous incompressible medium shifted between coaxial cylinders along the common axis of symmetry and twisted around it by longitudinal displacement and axial rotation of the inner cylinder is given, respectively, under sticking conditions and given longitudinal pressure drop. In particular, it was found that the speed of transportation of the mixed mass cannot exceed the speed delivered by the supplied pressure, and the formula of the latter is transferred to the compressible medium as a special viscosity integral for a viscoplastic medium, where it serves as a generalization of known exact solutions. A similar solution for a compressible medium is being sought. The obtained analytical dependencies can be used to calculate the parameters of industrial briquette extruders operating in both the stiff extrusion mode and the semi-stiff and soft extrusion mode, differing in the moisture values of the briquetted mass and the values of the applied pressure.
Modelo SEM basado en valores organizacionales y capital intelectual: un estudio realizado en entidades del sistema financiero peruano
Authors: Geraldo Campos Luis Alberto , Soria Quijaite Juan Jesús , Tito Huamaní Pedro Leonardo
Published: March 2020

La presente investigación analiza el efecto que tienen los valores organizacionales en los tres tipos capital intelectual, capital humano, capital estructural y capital relacional. La importancia de estudiar estos constructos radica en la generación de conocimiento que revelan, la cual se ha convertido en un recurso fundamental para las empresas de la actualidad, llevándolas a preocuparse por la parte intangible de la organización, es por ello, que el capital intelectual se ha venido considerando paulatinamente debido a la contribución en el cumplimiento de los objetivos organizacionales. No obstante, los valores organizacionales, como base fundamental de la organización, está volviendo a resurgir, debido al gran aporte y soporte que brindan para el gremio empresarial, desde allí radica la necesidad de estudiar estos dos constructos. El estudio se realizó bajo la metodológico de Ecuaciones Estructurales (SEM), realizándose un análisis exploratorio y confirmatorio, estudiándose una muestra de 207 funcionarios de 15 entidades financieras, basándose en el instrumento del inventario de perfil de valores organizacionales (IPVO) y el instrumento de capital intelectual con la finalidad de determinar el efecto que tienen las variables endógenas sobre las exógenas. Los resultados arrojaron que los valores organizacionales tienen relación causal significativa con los tres tipos de capital intelectual, capital humano, relacional y estructural. Sin embargo, los valores organizacionales necesitan del capital humano para ser compartidos, divulgados, interiorizados, en todos los procesos involucrando al capital estructural y capital relacional.
Código de Gobierno Societario en la Argentina: análisis del nivel de cumplimiento
Authors: Barco Eliana , Briozzo Anahí Eugenia
Published: March 2020

Un Código de Gobierno Corporativo es un conjunto de normas, principios y recomendaciones de comportamiento y funcionamiento, considerados como las mejores prácticas para el buen gobierno de las organizaciones para mejorar la transparencia, divulgación y rendición de cuentas. En el presente trabajo se analiza el grado de cumplimiento del Código de Gobierno Societario en la Argentina, que es de presentación obligatoria para las empresas que hacen oferta pública de sus títulos. El trabajo empírico se lleva cabo en una muestra de 20 empresas de capitales nacionales, excluyendo al sector financiero y a las compañías que realizan cross listing de sus valores. Se determina el nivel de cumplimiento del código presentado por cada firma desde dos perspectivas: la autoevaluación realizada por la propia emisora, según el principio de cumplir o explicar, y el análisis efectuado por un evaluador externo, a partir de la información públicamente disponible. Los resultados muestran que el nivel de cumplimiento es mayor desde la óptica de la autoevaluación, y para las empresas de mayor tamaño. Los principios de mayor cumplimiento, independientemente del tamaño de la compañía, son los referidos a partes relacionadas y a la función de auditoría. En las firmas de mayor capitalización también resulta elevada la adhesión al principio de ética empresarial. El principio referido a remunerar de manera justa y responsable presenta el menor cumplimiento en todas las empresas estudiadas.
Análisis de validez de constructo del instrumento: “Enfoque Directivo en la Gestión para Resultados en la Sociedad del Conocimiento
Authors: Martínez-Corona José Isaías , Palacios-Almón Gloria Edith , Juárez-Hernández Luis Gibrán
Published: March 2020

En el presente trabajo se exponen los resultados de un estudio instrumental que aborda el análisis de la validez de constructo y confiabilidad del instrumento: enfoque directivo en la gestión para resultados en la sociedad del conocimiento, con el objeto de que obtenga una calidad óptima para que brinde evidencias válidas y confiables. La construcción del instrumento se basó en los cuatro ejes fundamentales del ciclo de gestión y en lo expresado en el primer principio de la Declaración de Marrakech. Para cumplir con el objetivo, el instrumento fue aplicado a 505 directivos de la administración pública. El análisis de validez de constructo se realizó con la técnica del análisis factorial exploratorio (AFE), verificándose la pertinencia de los datos para esta técnica y se analizó la confiabilidad del instrumento mediante el Alfa de Cronbach. Los resultados del AFE revelan que los ítems están representados en el modelo factorial, manifestándose un solo factor, lo cual corresponde con el modelo teórico propuesto. En cuanto al análisis de confiabilidad, se obtuvo un valor óptimo (Alfa de Cronbach: 0.868). Ante estos resultados, se concluye que el instrumento y los elementos que lo integran representan y miden con precisión el constructo que se pretende evaluar
Генотипическая изменчивость аминокислотного состава белков животного и растительного происхождения
Authors: Рудаков Олег Борисович , Рудакова Людмила Васильевна , Букша Максим Сергеевич
Published: March 2020

В статье рассматриваются статистические данные по аминокислотному составу белков жи-вотных и растений, полученные методами ионообменной и высокоэффективной жидкостной хрома-тографии. Изучали состав аминокислот мяса разных животных и сои разных сортов. Для статистиче-ского анализа использовали экспериментальные данные по аминокислотному составу белков мясабройлеров (N=208), баранины (N=162), мяса кролика (N=65), свинины (N=52), мяса гуся (N=68), го-вядины (N=46), мясо кенгуру (N=10) и креветки (N=68), изолята сои (N=234). Обсуждаются выявлен-ные биохимические корреляции, наблюдаемые при изменении аминокислотного состава белков в за-висимости от их происхождения. Существенные прямые (симбатные) корреляции (R=0.70-0.90) на-блюдаются между содержанием глицина, аланина и пролина с изменением состава мясных белков.Содержание фенилаланина коррелирует с содержанием аланина, тирозина и аргинина. Более тесныекорреляции (R>0.9), наблюдаются между аминокислотами в соевом белке. Однако эти корреляцииотличаются от корреляций, характерных для животных белков. Установлены универсальные соотно-шения аминокислот в белках как животного, так и растительного происхождения. Это соотношениямежду глицином, аланином и фенилаланином, между фенилаланином и тирозином. В статье сравни-ваются контрольные диаграммы аминокислотного состава соевого изолята, говядины и баранины.Показано, что из-за незначительных различий в аминокислотном составе мяса и соевого изолята, порезультатам аминокислотного анализа трудно идентифицировать фальсификацию мясных продуктов,в которые добавлен соевый белок в количестве менее 25%. Тем не менее, найденные корреляции мо-гут быть полезны для определения происхождения белка и выявления грубой фальсификации мясныхпродуктов. Они также могут быть использованы в биологических, биохимических и диетологическихисследованиях.
Специфика финансовых механизмов местного самоуправления в России
Authors: Мысляева Ирина Николаевна , Науменко Тамара Васильевна
Published: March 2020

Статья посвящена выявлению основ проявления негативных тенденций в развитии местного самоуправления в России. Внимание обращено на то, что во многих регионах муниципальные бюджеты по-прежнему остаются дефицитными, и это явление начинает принимать хронический характер. Основной исследовательской задачей статьи является выявление глубинных причин сложившейся ситуации в области формирова­ния местных бюджетов. Обобщая опыт их формирования на протяжении последних двадцати лет, авторы приходят к выводу, что тенденции обусловлены особенностями развития местного самоуправления. Именно данный вывод позволяет понять почему преимущественно дефицитное состояние местных бюджетов не может быть сглажено частичными реформами системы налогообложения или совершенствованием механизмов межбюджетного выравнивания на субрегиональном уровне. Наличие негативных тенденций в развитии местного самоуправления обусловлено игнорированием теоретических представлений относительно самой природы местного самоуправления и экономических механизмов его реализации. Чтобы решить практическую задачу, связанную с наполняемостью местных бюджетов, необходимо, в первую очередь, уточнить ряд важных теоретических вопросов. В частности, обратиться к выявлению сущности местного самоуправления как особой сферы управления, отделенной от государственного управления, выявить особенности формирования и использования финансовых ресурсов, которыми местное сообщество распоряжается самостоятельно. Только на этой основе можно сформировать устойчивую финансовую базу для развития местных сообществ в России. Авторами также сделан вывод о том, что финансовые механизмы местного самоуправления нуждаются в коренном реформировании, прежде всего на институциальном уровне. Выявлено, что решение этой задачи может быть обеспечено за счет развития процессов демократизации бюджетного процесса на местном уровне, а также посредством формирования новых институтов, способствующих реализации этой цели.
nCoV-2019 sequencing protocol for illumina v1
Authors: Itokawa Kentaro , Sekizuka Tsuyoshi , Hashino Masanori , Tanaka Rina , Kuroda Makoto
Published: March 2020

This protocol is folked from "ARTIC amplicon sequencing protocol for MinION for nCoV-2019" by Josh Quick to adapt it to illumina sequencers. Because the PCR products are fragmented and ligated with adapters, this protocol is not restricted to 250 PE mode of MiSeq. While the library preparation uses QiaSeq FX by Qiagen and is basically straight forward (as par kit instruction but set to 1/4 scale), some tweaks for much of simplicity and speed were added.
Current approaches to assessment of the impact of the environmental contamination on cancer risk
Authors: Solenova L. G.
Published: March 2020

The current estimates of the contribution of the environmental contamination to cancer mortality range from 1 to 20 %, being a function of the ecological conditions, the density of industrial enterprises, occupational composition of the population, and others. Assessment of the impact of environmental contamination on cancer risk includes: •setting research priorities on the local, regional, and on the all-Russian levels; •the selection of the research that may be maximally socially profitable; •the choice of the research method that the most adequately meets the research objectives; •systemic analysis of the planed research to determine the availability of the resources, personnel, and information; •monitoring of atmospheric pollutions with analysis of the fraction composition of the particulate matters; •coordination of available data basis on environment and the population health conditions; •the implementation of the of molecular biology to determine prenosological manifestation of carcinogenesis and development of fine and diverse research methods on relationships between the environment and cancer risk.

For effective implementation of the research objectives aimed to decrease the impact of hazard factors with special reference on cancer risk in the Russian population, it is necessary: to create training personnel capable of providing epidemiologic studies, using up-to day methods, publication of methodological materials, text books, and sufficient funding of studies.
The importance of preexisting drug resistance due to overexpression of P-glycoprotein for the formation of resistance to bortezomib
Authors: Laletina L. A. , Moiseeva N. I. , Karamysheva A. F.
Published: March 2020

Objective of the study. In our work we investigated the effect of pre-existing drug resistance by the mechanism of activation of ABC transporters – P-glycoprotein (Pgp) overexpression – on the development of resistance to the proteasome inhibitor bortezomib.

Materials and methods. Cultures RPMI8226 and K562 / i-S9 (with Pgp overexpression) and their bortezomib-resistant sublines RPMI8226 / btz-6 and K562 / i-S9vlc were used as models. The methods used were MTT test, flow cytometry, Western blot and real-time polymerase chain reaction using the Human Signal Transduction Pathway Finder system.

Results. The expression of the main PI3K-AKT and NF-κB signaling pathways did not change in RPMI8226 / btz-6 subline cells. However, AKT kinase expression was significantly increased and PTEN protein expression was reduced in K562 / i-S9vlc cells with Pgp-overexpression. Significant changes in gene expression (42 %) were found in RPMI8226 / btz-6 cells related to a number of main signaling pathways in the tumor cell, namely: activation of 3–4 genes in signaling pathways related to hypoxia, oxidative stress, PPAR and p53. The highest activation in these cells was found in the TGFβ signaling pathway. In resistant K562 / i-S9vlc cells, expression of only 5 genes (10 %) increased: Fas, HMOX1, CPT2, ICAM, and SOCS3. Three genes were also identified that changed in both resistant sublines: Fas, HMOX1 and CPT2. Further, we showed that in the RPMI8226 / btz-6 subline, along with changes in the expression of signal transduction genes, there is a large pool of CD138-negative cells, and in the K562 / i-S9vlc subline, the number of cells expressing CD34 increases and the number of CD13 decreases.

Conclusion. We found that different signaling pathways are involved in the formation of resistance to bortezomib in the absence of Pgp expression and its overexpression. In addition, a cell line without activated resistance pathways requires more extensive rearrangements in the signal system to acquire resistance to bortezomib. However, in both cases, bortezomib leads to a change in the immunophenotype of the cells – to the appearance of dedifferentiated subpopulations.
GENESIS OF THE THERMAL-MINERAL WATERS OF ILIDŽA, BOSNIA AND HERZEGOVINA
Authors: Vlahović Tatjana , Skopljak Ferid
Published: March 2020

The springs of thermal-mineral waters (TMWs) of Ilidža near Sarajevo appear at the transition of the Central and Inner Dinarides of Bosnia and Herzegovina, at the overthrust front of the Bosnian Flysch and the far southeastern part of Sarajevo-Zenica Tertiary Basin. The wider area of Ilidža is composed of Mesozoic and Cenozoic deposits. The genesis of the TMWs of Ilidža was determined from the structural-tectonic relationships and hydrogeological features of the terrain, as well as from the analysis of hydrogeochemical indicators and isotopic compositions of water and gases. Hydrogeochemical methods included analysis of the hydrocarbon indicators, ionic ratio calculations and genetic classifications. The primary aquifer of TMWs of Ilidža are Permian-Triassic sediments with gypsum and anhydrites, and secondary aquifers are Triassic carbonates and alluvial deposits. The temperature of the TMWs resulted from the interaction of infiltration depth of atmospheric water and the geothermal gradient. Static pressure in the lenses of Permian-Triassic deposits and the dynamic factors of TMWs, moving and ascending through porous rocks over long distances under extremely high pressures, contributed as well. The TMWs are of atmospheric origin and had no interaction with the atmospheric or surface waters since the year of 1953. TMWs are formed in the Upper Pleistocene, at the time of the cold climate within the epoch. Sulphates in TMWs originated from the Permian-Triassic evaporites. H2S is produced under sulphate reducing conditions and CO2 is produced from metamorphosed carbonates.
IDENTIFCATION OF HIGH VELOCITY ANOMALIES, IMPERCEPTIBLE TO SEISMIC RESOLUTION, BY INTEGRATION OF SEISMIC ATTRIBUTES, IN PENOBSCOT FIELD, CANADA
Authors: García Moreno Wilmer Emilio , Omaña Galeano Iván Daniel
Published: March 2020

Penobscot Field is located in the Sable Sub-basin, in the Nova Scotia Basin, belonging to the Canadian territory, and for the year of 1991, a 3D seismic acquisition was carried out in this region. Also, two exploratory wells were previously drilled (L-30 and B-41). Then, with the subsequent interpretation of the area, a discrepancy was found around 10ms to 15ms (approximately 25m) in the travel times of the seismic waves in the vicinity of well B-41, resulting in a false structural high in the Mississauga Formation and Naskapi Member. It is possibly caused by changes in geological facies and thickness of the Wyandot formation, consequently, B-41 well became dry. For these reasons, to identify the possible problem which caused everything mentioned above, the area between the wells was delimited by a seismic subvolume (by reducing the seismic cube), making a seismic interpretation of the top of the formations of interest, as well as an analysis of certain petrophysical properties in the wells, in order to delimit the thickness of the anomaly and, consecutively, the use of the seismic attributes for lateral identification of the anomaly in the Wyandot Formation.
SYSTEM ARCHITECTURE TO BRING SMART PERSONAL PROTECTIVE EQUIPMENT WEARABLES AND SENSORS TO TRANSFORM SAFETY AT WORK IN THE UNDERGROUND MINING INDUSTRY
Authors: Adjiski Vancho , Despodov Zoran , Mirakovski Dejan , Serafimovski Dalibor
Published: March 2020

As safety at work has become a number one priority in every industry, it is no wonder that new technology and ideas are being tested to improve safety in a variety of workplaces, including, surface and underground mining, construction sites, power plants, factories, etc. Sensor technologies embedded in personal protective equipment (PPE), can be employed to monitor workers’ health, exposure to harmful elements, their proximity to danger zones, etc. This technology connected to smartphones and smartwatches can also heighten workers’ awareness by collecting data on the workplace itself, detecting environmental and health hazards. In this paper, we will present a prototype system that can be employed in underground mining which uses sensors attached to regular PPE clothing, including hard hats and safety glasses which are connected to smartphones and smartwatches via energy-efficient Bluetooth sensors, to provide real-time safety situation awareness and predict health and safety incidents before they occur.
APPLICATION OF GIS PROCEDURE FOR RIVER TERRACE EXTRACTION FROM A LiDAR- BASED DIGITAL ELEVATION MODEL: THE SAVA RIVER VALLEY NW OF ZAGREB, CROATIA
Authors: Trenc Neven , Matoš Bojan , Velić Josipa , Perković Dario
Published: March 2020

River terraces and floodplains indicate channel-sediment dynamics and represent important landform features, which can be used to untangle the evolutionary history of climate and tectonic conditions in a certain area. With the availability of high-resolution topographic data, it has recently become feasible to apply GIS procedures for the identification of these subtle landform features. In this study, the TerEx Toolbox, a GIS extension for semiautomatic terrace extraction was applied on the LiDAR based DEM of the Sava River, west of Zagreb in Croatia. The aim of this research was to compare results obtained by TerEx to the existing geological map from the 1970s, which shows sediments of the recent flow (routinely flooded area) and two abandoned Holocene river terraces in the study area. The TerEx output was validated using hillshaded DEM, a historical map of the study area, orthophoto imagery, topographic cross sections and field investigations. TerEx has successfully extracted Holocene terraces and risers shown on the geological map but in some parts of the study area, their position and extent was different. The presented study shows that results obtained by TerEx, in particular if used in combination with other data sources, can effectively focus fieldwork and contribute to new unbiased interpretation of terrain morphology.
Problems of Interpretation of Paragraph 2 of Article 35 of the Family Code of the Russian Federation in Russian Legal Science and Jurisprudence
Authors: Dobrovinskiy A. A.
Published: March 2020

The paper analyzes the issues of interpretation and application of the presumption of spousal consent in transactions involving the common property of spouses. Presumption of spousal consent to carry out transactions with common property is considered in the context of its correlation with the principle of equality of spouses, including property relations. The paper deals with the jurisprudence on this issue, including the decisions of the Supreme Court of the Russian Federation. The author has determined practical problems in the field of application of this rule of law. The author comes to the conclusion that the legal regulation of the legal regime of property of spouses, in force at present, has a number of shortcomings that not only violate the rights of co-owners of the specified property, but also, in our view, are often contrary to the law. In particular, the current jurisprudence with regard to paragraph 1 of section 2 of Article 35 of the RF Family Code contradicts both the constitutional rule enshrined in Article 35 of the Constitution of the Russian Federation providing for the protection of the interests of the owner and the norms of the Family Code of the Russian Federation establishing the principle of equality of spouses in property relations (Articles 21, 31, 35). The paper gives reasonable proposals to improve the legislation. To this end, the author suggests the following wording for Paragraph 1 of Section 2 of Article 35 of the Family Code of the Russian Federation: “When one of the spouses carries out a transaction in the administration of the common property of the spouses, it is assumed that one spouse acts with the consent of the other spouse. This assumption applies exclusively to the legal relationship of spouses with third parties.”
Ontology (Existence) of Law in Modern Realities of Legal Regulation of Socially Oriented Economy
Authors: Popova A. V.
Published: March 2020

The paper analyzes philosophical and philosophical and legal approaches to the definition of such concepts as ontology of law and existence of law in the history of world legal thought. The author has elucidated the views on the ontology of law in the history of the Ancient World, Middle Ages, New and Modern Times. Thus, the doctrine of existence was central to the philosophical and legal constructions of Antiquity. In the Middle Ages, ontology was associated only with God, in the era of modern times the existence of law, depending on the philosophical and legal doctrine, was defined as an absolute idea, as a manifestation of the will of the State, as rights of the individual, or as the Divine Providence. Particular attention is paid to the analysis of various approaches to the definition of the content of ontology (existence) of law in economic law as a new innovative component of the Russian system of law in the era of an information society. Particular attention is paid to the interpretation of ontology of law in natural legal and positivistic doctrines of legal understanding, the general and special features of the content of ontology of law in philosophical and legal doctrines of the past. The 19th-20th centuries witnessed the development of a “new philosophy” focused on scientific and technological progress creating a new economic reality, when the existence of law is presented as an object of ontology in the unity of interpretations of the objective and subjective giving the opportunity to know the reality in its various hypostasis. The legal environment as a subject matter of the philosophy of law at the present stage of development of the digital economy focuses primarily on propaedeutics and characterization of property rights as a necessary condition of the development of modern society. The author has come to a reasonable conclusion that in the framework of the Russian ontology of law it is necessary to apply a converging approach in order to strengthen social orientation of the Russian economy with due regard to integrative legal understanding.