bluuebunny/crossref_metadata_2025 huggingface dataset:
NUTRITIONAL SURVEY IN CRITICALLY ILL CHILDREN AND THEIR IMPACT ON ANTHROPOMETRIC INDICES AND OUTCOME
Authors:   Assistant Professor in Department of Pediatrics, BJGMC and Sassoon General Hospital, Pune., Deshmukh Isha , Lanjewar Ketaki ,   Pediatric Resident, BJGMC, Pune., Sonkawade Naresh ,   Naresh Sonkawade Assistant Professor in Department of Pediatrics, MD Pediatrics, BJGMC and Sassoon General Hospital, Pune., Kinikar Aarti ,   Professor and Head of the Department, MD Pediatrics, Department of Pediatrics, BJGMC and Sassoon General Hospital, Pune.
Published: January 2025

Malnutrition has a high incidence among patients hospitalized to PICUs. It has a major impact on a childs outcome, including death, morbidity, and length of ICU stay.1,2,3 During this acute phaseinICU,there is decreased energy and protein delivery to body tissues. So the metabolism shifts to catabolic. Although initially these changes are life saving for the child but as a whole these changes deteriorate the nutritional status of the child. And if it coupled with suboptimal nutrition intake during ICU stay then it leads to significant anthropometric deterioration.,

Objectives:

1.To evaluate incidence of malnutrition in critically ill children admitted to PICU.

2.To find the change in anthropometric indices mainly weight in critically ill children after illness phase in PICU.

3.Impact of enteral nutrition on outcome of child.

Study Design: Prospective observational study conducted in pediatric intensive care unit over a period of 6 months from 1/02/2024 to 31/07/2024.

Results and Conclusions: The findings demonstrated that malnutrition was prevalent among children admitted to PICU. Girl children were especially affected more than boys.Early start of enteral nutrition (within 24 hours) can reduce the overall mortality rates and ICU stay in normal and malnourished children therefore the practice must be encouraged with proper protocols especially in intensive care units.

Limitations of the study: Our current study has limitations that include small sample size, patient heterogenicity, variable disease severity, lack of information on baseline nutritional status and insufficient statistical power for analysis.
A LINGUISTIC STUDY OF REFUSAL STRATEGIES USED BY THE UNIVERSITY OF HAILS UNDERGRADUATE STUDENTS
Authors:   University of Hail, Hail, Saudi Arabia., Thaher Ibrahim
Published: January 2025

The present study examines the refusal strategies used by the students at the University of Hail (UOH). It tackles two main questions related to what refusal strategies, classified by Beebe et al. (1990), Saudi students at the University of Hail (UOH) employ in their refusal responses and their reasons for using the refusal strategies. The current study has used a modified written discourse completion test (DCT). The students are asked to provide written responses to express their refusals to the given situations. The collected data are analyzed according to the content, number of students, and order of formulas employed by Beebe et al. (1990). The findings indicate that Saudi students at the (UOH) use more expressions of excuses/ reasons/ explanations followed by expressions of regrets. In many cases, they avert the use of no in their refusal strategies, except in some cases where it is followed by excuses or explanations. Moreover, this study reveals that Saudi students have three reasons for justifying their refusals indirectly. These reasons are respect, face-saving, and indirect communication.
Expanding portfolio diversification through cluster analysis beyond traditional volatility
Authors: Kuzheliev Mykhailo , Zherlitsyn Dmytro , Rekunenko Ihor , Nechyporenko Alina , Stabias Sergii
Published: January 2025

The study reviews the application of machine learning tools in financial investment portfolio management, focusing on cluster analysis for asset allocation, diversification, and risk optimization. The paper aims to explore the use of clustering analysis to broaden the concept of portfolio diversification beyond traditional volatility metrics. An open dataset from Yahoo Finance includes a ten-year historical period (2014–2024) of 130 actively traded securities from international stock markets used. Dataset selection prioritizes top liquidity and trading activity. Python analytical tools were employed to clean, process, and analyze the data. The methodology combines classical Markowitz optimization with clustering analysis techniques, highlighting variance-return trade-offs. Various asset characteristics, including annualized return, standard deviation, Sharpe ratio, correlation with indices, skewness, and kurtosis, were incorporated into the clustering models to reveal hidden patterns and groupings among financial assets. Results show that while clustering enhances insights into asset diversity, classical approaches remain historically superior in optimizing risk-adjusted returns. This study concludes that clustering complements, rather than replaces, classical methods by broadening the understanding of diversification and addressing many diversity factors, such as metrics of the technical, graphical, and fundamental analysis. The paper also introduces the diversity rate based on clustering, which measures the variance balance by all features within and between clusters, providing a broader perspective on diversification beyond traditional metrics. Future research should investigate dynamic clustering techniques, integrate fundamental economic indicators, and develop adaptive models for effective portfolio management in evolving financial markets.
Complexity of Conflict for Islamic Educational Management Student; Humanistic Approach as a Solution
Authors: Defi Wahyu Fitrina , Gistituati Nurhizrah
Published: January 2025

The realm of Islamic educational management is not immune to conflicts, which can arise from various sources such as differing ideologies, cultural disparities, or administrative challenges. This study delves into the multifaceted dimensions of conflicts faced by Islamic educational management students. By employing a humanistic approach, this research seeks to unravel the intricate layers of conflicts within the context of Islamic education and offers innovative solutions to mitigate their adverse effects. Drawing from theories rooted in humanism, the study explores the psychological and sociocultural aspects of conflict resolution, emphasizing empathy, understanding, and effective communication. Through in-depth qualitative analysis and case studies, the research provides valuable insights into the root causes of conflicts, enabling Islamic educational management students to cultivate a harmonious learning environment. This study not only contributes to the academic discourse but also equips future leaders in Islamic education with practical strategies to navigate conflicts, fostering a conducive atmosphere for holistic learning and personal growth.
Implementasi Manajemen Konflik Intrapersonal dan Interpersonal oleh Murobbi bagi Santri Pondok Pesantren
Authors: Mustafidin Ahmad , Wahyudi Andi , Ambari Muhammad Zaki
Published: January 2025

The main goal of Islamic education is to form good morals or character. The rapid development of technology has become a new chapter in efforts to develop an Islamic education system so that it can always follow the flow of developments. In the current era of globalization, it has become a necessity for Islamic educational institutions to update educational services according to the needs of the times. Ideally, Islamic educational institutions in the 21st century must follow the flow of developments and not abandon the concept of traditional Islamic education (pesantren). In this case, education cannot be separated from the role of a teacher or murobbi as the spearhead of the Islamic education system. In Islamic education, we know the term murobbi as a physical and spiritual educator. This study will discuss, 1) how to identify intrapersonal and interpersonal conflicts in the Darul Amanah Bedono Islamic boarding school? 2) how is the implementation of intrapersonal and interpersonal conflict management in the Darul Amanah Bedono Islamic boarding school? This study uses a qualitative descriptive method, where this method is used to present in-depth information related to the implementation of murobbi intrapersonal and interpersonal conflict management for students. The results of this study illustrate that the murobbi system is easier to implement intrapersonal and interpersonal conflict management, including the following; Intrapersonal conflict management begins with identifying individual problems of students, including; pessimistic, unfocused, bored, loss of motivation, undisciplined, confused, needing adjustment, and lazy. While conflict management or problem solving that is applied includes; providing motivation, conducting summons, focus training, outings, and refreshments. The application of interpersonal conflict management is carried out by identifying conflicts between students such as; physically insulting each other, belittling parents' work, insulting appearance, and offending feelings. While the problem solving that is applied includes; socialization, summons, coaching, confrontation, motivation, time discipline, direction, games, discussions, mutual respect, education, respect, and providing understanding. This study opens up opportunities for researchers such as lecturers and students as well as the community who need the results of research that has been conducted on research topics that are relevant to this study. It is hoped that for the sake of progress in the field of research, further researchers can use different research methods, or explore other relevant and objective variables.
Analisis Eektivitas Gaya Kepemimpinan Demokratis Kepala Sekolah dalam Manajemen Konflik di Lingkungan Pendidikan
Authors: Musfiroh Khofifah Aini , Fanani Ahmad Falasul , Kurniawan Bima , Amaniyah Kamilia , Mu' Alimin Mu' Alimin
Published: January 2025

This study aims to analyze the effectiveness of the democratic leadership style of school principals in managing conflic in educational environments, as well as provide an understanding of how school principals develop an effective leadership approach that is in accordance with legal norms and the moral values of society. This research employs a qualitative method, focusing on literature review as the object of study, which involves data collection by searching for and exploring relevant literature related to the formulation of the problem. The results of the study indicate that the democratic leadership style of school principals has a positive impact on managing conflict, by increasing active participation and collaboration in decision making. This leadership style also creates open communication, mutual trust, and shared responsibility that can reduce tension and make conflict a source of ideas and innovation. In addition, democratic leadership style contributes to increased performance, job satisfaction, and creates a positive work atmosphere, which ultimately supports the achievement of educational goals. This study also emphasizes the importance of implementing a democratic approach in increasing teacher and staff involvement and motivation. Suggestions for further researchers are to explore the impact of democratic leadership style on other aspects of school management, and to conduct additional studies to evaluate the effectiveness of democratic leadership in crisis situations. This is important to obtain a more comprehensive picture of its influence on the quality of education, as well as to understand the limitations of the approach.
From Silence to Strength: A Women-centered Framework in Faith-based Community Organizing
Authors: Hamidah Kamilia , Hussin Sabariah , Naharin Sri
Published: January 2025

Three models of community organizing (CO) are commonly used as paradigms: the conventional Alinsky’s, faith-based community organizing (FBCO), and the women-centered model. However, few studies on CO that focus on integrating these three models as a comprehensive lens of analysis. The women-centered community organizing paradigm lacks religio-spiritual complexity, which hinders the acceptance of women empowerment’s message among religious communities. This study aims to propose a practical community-organizing model by synthesizing three different community-organizing models carried out by religious-based women non-governmental organizations. This study combines a systemic literature review method and a qualitative approach to study women-based CSOs. The finding indicates that integrating three community organizing (CO) models effectively empowers women.
Pioneering Change: Women’s Economic Empowerment under Saudi Vision 2030
Authors: Jamilah Maryam , Isnarti Rika
Published: January 2025

Women's empowerment efforts always encounter obstacles and challenges. This paper explores the economic empowerment of women in Saudi Arabia within the context of Saudi Vision 2030, a plan aimed at enhancing women's roles in various sectors. Although existing research has addressed women’s empowerment under this vision, a comprehensive evaluation of the government’s initiatives is still lacking. The study evaluates policies designed to economically empower women, guided by a theoretical framework based on key empowerment elements: agency, access to resources, opportunity structure, and achievement, inspired by the perspectives of Evelyn Derera and Amartya Sen. A qualitative, descriptive approach is used, incorporating secondary data analysis from literature reviews and government reports on Vision 2030. Findings reveal that reforms have increased women’s participation in economic and educational sectors, including partial removal of the male guardianship system. However, challenges such as cultural norms, nepotism, and gender discrimination continue to limit women’s access to higher leadership roles. Despite significant progress, more efforts are required to address these persistent barriers and fully empower women in Saudi Arabia.
Atividade ovicida in vitro de folhas de Mentha spicata L. em helmintos gastrointestinais de ovinos
Authors:   , Rebouças Cristina Karine de Oliveira
Published: January 2025

A ovinocultura é tida no Brasil como uma importante atividade socioeconômica,
principalmente na região Nordeste. Contudo, uma das principais limitações para a
criação desses pequenos ruminantes seriam as parasitoses, destacando as causadas por
helmintos gastrointestinais. Essas infecções provocam diversos prejuízos a esses
animais, como perda de peso, quadros de anemia, redução do potencial de produção,
podendo ocasionar quedas da taxa de produtividade dos mesmos. Os fitoterápicos
surgem como uma alternativa, tanto para o tratamento desses parasitos como para o
controle da resistência parasitária causada pelo uso indiscriminado de anti-helmínticos
químicos comumente usados no tratamento de ovinos. Dessa forma o objetivo da
pesquisa foi avaliar in vitro a atividade ovicida das folhas de Mentha spicata L. em
helmintos gastrointestinais de ovinos. Para tal, folhas de M. spicata foram coletadas,
secas a temperatura ambiente e processadas para preparo do extrato salino e análise
fitoquímica. Quanto à atividade anti-helmíntica foram realizadas coletas em pool
amostral de 10% do rebanho ovino, no qual realizou-se a contagem de ovos por grama
de fezes para comprovação do nível da infecção, seguido pela recuperação dos ovos e
pelo Teste de Eclosão de Ovos (TEO). Para efeito toxicológico do extrato foi utilizado
bioensaio com Artemia salina. O extrato inibiu a eclosão de ovos em 79%, 78%, 40% e
23% para as concentrações de 80 mg/mL, 40 mg/mL, 20 mg/mL e 10 mg/mL,
respectivamente. As concentrações com melhor ação ovicida foram 80 mg/mL e 40
mg/mL, entretanto a 80 mg/mL foi considerada tóxica (CL50= 59.04 mg/mL), indicando
o extrato de 40 mg/mL como a alternativa mais viável. Na análise fitoquímica a
presença de fenóis, saponinas, núcleos esteroidais e taninos hidrossolúveis foram
identificadas, podendo estar relacionados à atividade ovicida do extrato. Conclui-se que
o extrato da M. spicata L. apresentou substâncias bioativas, com efeito anti-helmíntico
em ovos de helmintos gastrointestinais de ovinos.
Análise multicritério como subsídio ao zoneamento ambiental de um parque nacional no bioma Caatinga, Brasil
Authors:   , Medeiros Francisco Hiályson Fidelis
Published: January 2025

Esta pesquisa visa subsidiar a proposição de um Zoneamento Ambiental para o Parque Nacional
da Furna Feia e sua Zona de Amortecimento, baseado em avaliação multicritério. Os objetivos
específicos deste estudo foram: i) realizar o levantamento, mapeamento e caracterização de
critérios espaciais, voltados aos aspectos ambiental, social e econômico do Parna Furna Feia e
sua Zona de Amortecimento; ii) realizar uma análise multicritério para o Parna Furna Feia e sua
Zona de Amortecimento; iii) construir uma proposta de Zoneamento Ambiental para o Parque
Nacional da Furna Feia, usando a análise multicritério; e iv) propor uma setorização
socioeconômica para a Zona de Amortecimento do Parque Nacional da Furna Feia, com base
nos resultados da análise multicritério. A coleta, tratamento e espacialização dos dados foram
realizadas em ambiente de Sistema de Informação Geográfica. Foram definidos cinco objetivos
de criação do Parna Furna Feia: (i) preservação do complexo espeleológico, (ii) da
biodiversidade, (iii) desenvolvimento de pesquisas científicas, (iv) educação e turismo e (v)
desenvolvimento socioeconômico das comunidades inseridas na Zona de Amortecimento,
sendo selecionados dois critérios gerais e cinco critérios específicos para cada objetivo. Para a
análise multicritério, todos os critérios identificados foram mapeados, tratados, ponderados por
meio do Analytical Hierarchy Process (AHP) e combinados. Após a avaliação multicritério, os
dados foram avaliados por álgebra de mapas, com o intuito de propor o zoneamento para o
Parna Furna Feia e sua Zona de Amortecimento. Foram espacializados e caracterizados 15
critérios e 25 subcritérios, que resultaram em cinco mapas de aptidão e um mapa de aptidão
integrada. A análise desses dados permitiu elaborar uma proposta de Zoneamento Ambiental
para o Parna Furna Feia e uma setorização para a sua Zona de Amortecimento. Foi possível
elaborar cinco zonas para o Parna da Furna Feia e sete setores para a Zona de Amortecimento.
Com base na análise de álgebra de mapas, a sobreposição das zonas aos mapas de aptidão
permitiu inferir justificativas para a seleção e caracterização destas zonas e assim obter um
zoneamento por análise multicritério.
Percepção do uso e atividade desinfetante de libidibia ferrea
Authors:   , Holanda Jamile Rodrigues Cosme de
Published: January 2025

A população vive exposta a uma diversidade de micro-organismos que estão presentes no meio ambiente com os quais convivem diariamente. Podendo tais microorganismos ser patogênicos ou não. A fim de controlar estes micro-organismos nas superfícies, realiza-se a desinfecção, com produtos químicos. Estes, por sua vez, vêm ocasionando agravos ao meio ambiente e saúde, bem como proporcionando o desenvolvimento da resistência destes micro-organismos. Desta forma, surge a necessidade do emprego de medidas alternativas naturais. Visto que a Libidibia ferrea é uma árvore que tem grande potencial medicinal, busca-se analisar as propriedades inibitórias do extrato e decocto das folhas da L. ferrea, e os componentes fitoquímicos, como também o conhecimento e aceitação das comunidades sobre esse desinfetante alternativo e sua eficácia como desinfetante. Para tal, adotou-se as seguintes metodologias: técnica de disco difusão e curva de crescimento sob a ação do jucá para a avaliação da atividade antimicrobiana; teste de avaliação desinfetante nas superfícies plásticas, aço inox e cerâmica e prospecção fitoquímica, bem como, a aplicação do teste de aceitabilidade do produto e do questionário para avaliar as formas de conhecimento sobre o uso do jucá, nas comunidades rurais de tradição do município de Mossoró/RN. A analise estatística deu-se pelo programa SigmaPlot, por One-Way ANOVA e One-Way ANOVA RM seguidos de Tukey, Kruskal-Wallis e Friedman. Para a analise sensorial foi usado o Wilconxon, sendo os valores de p>0,05 significatidos. Obtendo como resultado na técnica de disco difusão em poços a sensibilidade da S. aureus e E. feacalis ao decocto e para o extrato a P. aeruginosa, Micrococcus spp., Corynebacterium spp., S. Typhirium, S. aureus e E. feacalis. Quanto a curva de crescimento avaliada após as 24 horas, essa demostrou uma redução do valor de absorbância para o extrato a P. aeruginoa, Corynebacterium spp., S. Typhirium e E. feacalis, para o decocto somente a ultima. No teste da ação desinfetante nas superfícies comprovou-se que houve uma redução bacteriana para o decocto como para o extrato considerando o controle negativo em todas as superfícies, com exceção da S. Typhimurium em relação ao aço. Quanto à fitoquímica evidenciou-se a presença de flavonóides, saponinas, taninos, cumarinas, esteroides e compostos fenólicos. Observou-se também que em todos os questionários avaliados há uma maior aceitação do desinfetante a base das folhas da L. ferrea. Desta forma pode-se afirmar que o desinfetante a base de jucá é uma alternativa natural eficaz e bem aceita pela população.
Atividade ovicida in vitro do extrato salino das folhas do ziziphus joazeiro mart. Em nematoides gastrintestinais de caprinos
Authors:   , barbosa Tallyson Nogueira
Published: January 2025

A caprinocultura é uma atividade agropecuária de grande importância econômica e
social. Entretanto, o parasitismo por nematóides gastrintestinais ocasiona grandes perdas na
produção, associadas ao desenvolvimento da resistência química parasitária. Como essa
resistência tem-se agravado ao longo dos anos, a busca por métodos alternativos tornou-se
essencial para a atividade produtiva. O objetivo deste estudo foi analisar fitoquímicamente o
extrato salino obtido a partir das folhas de Ziziphus joazeiro Mart., avaliar sua atividade
ovicida contra parasitos gastrintestinais de caprinos (GIN) e sua toxicidade aguda frente a
náuplios de Artemia salina. Para tal, folhas de Z. joazeiro foram coletadas, secas a
temperatura ambiente e processadas para preparo do extrato salino. Uma vez obtido o extrato
a análise fitoquímica foi realizada. Para o teste de eclodibilidade in vitro, ovos de parasitos
GIN foram recuperados de amostras fecais coletadas em caprinos naturalmente infectados e
incubados em adição as diferentes concentrações do extrato. Um ensaio para avaliar a
toxicidade do extrato salino de Z. joazeiro foi realizado contra náuplios de Artemia salina.
Como resultados, foram identificados os componentes fitoquímicos: saponinas, taninos,
flavonoides e alcaloides. O extrato da folha de Z. joazeiro apresentou resultados satisfatórios
de inibição de 99,53%, 75,28% e 67,66% nas concentrações 20,288mg/ml, 10,144mg/ml e
5,072mg/ml respectivamente. O extrato apresentou uma CL50 de 10,14mg/ml no teste de
toxicidade. Esses dados revelam que extrato salino bruto de Z. joazeiro apresentou
substâncias consideradas bioativas, com efeitos antiparasitários sob ovos de parasitos
gastrintestinais de caprinos.
Turismo de observação de botos-cinza: implicações para a conservação de uma espécie ameaçada de extinção
Authors:   , Santos Josivânia Emanuelly Azevedo dos
Published: January 2025

O turismo de observação de botos-cinza (Sotalia guianensis), espécie ameaçada de extinção
no território nacional, é um dos principais atrativos da Reserva de Fauna Costeira de Tibau do
Sul (REFAUTS). No entanto, a adoção de práticas inadequadas, resultante da ausência de
uma gestão eficaz, tem comprometido a sustentabilidade desse turismo, tornando-o uma
relevante ameaça para o boto-cinza. Dessa forma, este estudo teve como objetivos: (i)
descrever quem são e o que pensam os condutores de embarcações turísticas para observação
de botos-cinza e (ii) avaliar, por meio do videomonitoramento, a conformidade do turismo de
observação de botos-cinza em relação à Lei Municipal nº 349/2007, instrumento que
regulamenta esta atividade na REFAUTS. A coleta de dados ocorreu entre 2014 e 2018,
totalizando 40 dias de amostragem. Na REFAUTS, os condutores turísticos são
predominantemente jovens e têm o turismo de observação de botos-cinza como principal
fonte de renda. Ainda há pouco conhecimento entre estes profissionais sobre os aspectos
ambientais da REFAUTS, incluindo a Lei Municipal nº 349/2007. Contudo, estes condutores
turísticos declararam reconhecer a importância dos seguintes aspectos: boto-cinza, ambiente
marinho protegido, normas de conduta, programa de fiscalização e cobrança da taxa turística
para a REFAUTS. A técnica de videomonitoramento a partir da faixa de praia foi considerada
de baixo custo e eficaz para avaliar a conformidade do turismo de observação de botos-cinza
na REFAUTS. Em geral, esse turismo apresentou baixa conformidade com a Lei Municipal nº
349/2007, com o registro de pelo menos uma infração na maior parte dos passeios
monitorados. Os resultados deste estudo devem subsidiar um plano de ordenamento turístico e
viabilizar um melhor planejamento de políticas conservacionistas, assegurando a proteção do
boto-cinza na REFAUTS.
Condicionamento fisiológico em sementes de jucá (Libidibia ferrea (Mart. ex Tul.) L.P. Queiroz var. ferrea) submetidas a salinidade
Authors:   , Oliveira Valéria Nayara Silva de
Published: January 2025

Libidibia ferrea (Mart. ex Tul.) L.P. Queiroz var. ferrea), conhecida por jucá, pertence a família Fabaceae com ampla distribuição nas regiões Norte e Nordeste do Brasil. Essa espécie é utilizada para fins medicinais, construção civil e recuperação de áreas degradadas. Embora seja adaptada a região semiárida, suas sementes podem ter a germinação e o vigor comprometidos devido a salinidade da água ou do solo, condições muito comuns na região Nordeste do Brasil. Nesse contexto, objetivou-se avaliar a qualidade fisiológica de sementes de L. ferrea submetidas ao estresse salino, como também verificar os efeitos do condicionamento fisiológico como mitigador deste estresse. Para isso, foram realizados dois experimentos, sendo o primeiro em delineamento experimental inteiramente casualizado, em esquema fatorial 2 x 6, sendo dois lotes de sementes, submetidas a seis níveis de salinidade (0, 4, 8, 12, 16 e 20 dS m- 1), com quatro repetições de 25 sementes. As sementes foram semeadas em substrato tipo papel-toalha previamente umedecido com soluções de NaCl e colocadas para germinar em câmara de germinação a 25 °C, durante 20 dias. No segundo experimento, o condicionamento consistiu em colocar as sementes em substrato tipo papel toalha umedecido com soluções de polietielnoglicol (PEG 6000) nos potenciais osmóticos de - 0,2 e -0,4 MPa por 60 e 84 horas, respectivamente, e com água destilada por 36 horas a 25 °C. Em seguida, sementes com e sem condicionamento foram postas para germinar em substrato papel toalha umedecido com soluções de NaCl (12, 16 e 20 dS m-1) e água destilada (0 dS m-1) a 25 ºC. Em ambos os experimentos, as variáveis analisadas foram: primeira contagem, germinação, comprimento de plântulas (parte aérea e raiz) e massa seca (parte aérea e raiz). A resposta a salinidade variou entre os lotes de L. ferrea, sendo que a germinação foi afetada negativamente pelo aumento da salinidade em apenas um dos lotes, enquanto o vigor foi prejudicado a partir de 12 dS m-1 nos dois lotes. O hidroconcionamento e osmocondicionamento das sementes proporcionaram maior tolerância ao estresse salino em comparação às não condicionadas, até 16 dS m-1.
MARVELD1 Promotes the Invasiveness in Pancreatic Adenocarcinoma
through the Activation of Epithelial-to-Mesenchymal Transition
Authors: Luo Xianwei , Gao Zhenming
Published: January 2025

Background:

MARVEL domain-containing 1 (MARVELD1) has been implicated in
the progression of several cancers, but its role in pancreatic adenocarcinoma (PAAD) remains
poorly understood.

background:

Pancreatic adenocarcinoma (PAAD) is a highly malignant form of cancer that originates in the pancreas, with its exocrine cells producing digestive enzymes. MARVEL domain-containing 1 (MARVELD1) is a protein that plays a significant role in various cellular processes.

Methods:

RNA-seq data from the TCGA-PAAD and GTEx-Pancreas cohorts were analyzed to assess
MARVELD1 expression. Stable MARVELD1 knockdown and overexpression were conducted
in BxPC3 and PANC-1 cells. Cell viability, proliferation, migration, and invasion were evaluated
using functional assays, and western blotting was employed to examine EMT-associated protein
levels, including Vimentin, MMP2, MMP9, and E-cadherin. Differentially expressed genes
(DEGs) between MARVELD1-high and MARVELD1-low groups were identified, and pathway
enrichment analyses were performed.

Results::

We observed a significant increase of MARVELD1 in PAAD patient samples, with elevated
MARVELD1 levels correlating with poor clinical survival. Knockdown of MARVELD1 in
PAAD cells remarkably decreased cell proliferation and colony formation, while overexpression
of MARVELD1 enhanced these properties. Moreover, simulated cell invasion and migration assay
further suggested that MARVELD1 might contribute to PAAD cell aggressiveness. Mechanistically,
MARVELD1 promoted tumor cell migration and invasion through the activation of Vimentin,
MMP2, and MMP9 protein while suppressing E-cadherin. Bioinformatics analysis revealed
that MARVELD1-high samples were enriched in EMT-related pathways, including TGF-β
receptor signaling, actin cytoskeleton regulation, and cell adhesion.

Conclusion::

Taken together, our study highlights the roles of MARVELD1 in promoting tumor
cell proliferation and invasion, suggesting its potential application as a prognostic and diagnostic
biomarker for PAAD in the clinical context.

conclusion:

Taken together, our study highlights that MARVELD1 is a tumor-promoting gene and might be a prognostic factor and potential therapeutic target for PAAD in clinical.
Research Prospects for Necroptosis in Inflammatory Diseases: A
Bibliometric Analysis
Authors: Tan Ya ting , Wang Mei juan , Wang Shu chao
Published: January 2025

Background:

Necroptosis is a modifiable form of cell death mainly dependent
on RIPK3 and MLKL. The association between necroptosis and inflammation has
been a key focus of research. An increasing number of studies have shown that necroptosis
plays an important role in inflammatory diseases, such as inflammatory bowel disease.

Methods:

Articles published up to 2023 were searched on the Web of Science.
VOSviewer, CiteSpace, Gephi, and Microsoft Office Excel were used for bibliometric
analysis and visualisation. In addition, journal impact factors and journal partitions were
obtained through the Web of Science.

Results:

A total of 3011 articles were included in this study. The number of publications
and citations in the field increased year by year. China had the highest number of publications.
Cell Death & Disease published the most papers in the field. P. Vandenabeele is
one of the most important scholars in this field. The most cited reference was “Molecular
Mechanisms of cell death: recommendations of the Nomenclature Committee on Cell
Death”.We found substantial evidence that acute kidney injury, sepsis, cancer, and other
diseases are closely related to necroptosis. In addition, we found that inhibitors of necroptosis
have great potential in the treatment of inflammatory diseases.

Conclusion:

This is the first bibliometric analysis of studies related to necroptosis in inflammatory
diseases. Our results provide an overview of basic and influential research,
providing a basis for the identification of valuable research directions. Furthermore, this
work offers general insight into the role of necroptosis in inflammatory human diseases.

conclusion:

This study is the first to make a bibliometric analysis of the studies related to necroptosis in inflammatory diseases. Our research results
can provide the new coming researchers with the basic and influential research results to choose suitable and valuable research directions. Furthermore,
it could provide guidelines for necroptosis in inflammatory human diseases.
Metabolic Tagging Technology of Exosomes- An Updated Review
Authors: Sen Aniruddha , Mohanraj Palani Selvam , Nagaram Srinivas , Samanta Anita , Ashique Sumel , Islam Anas , Srivastava Shriyansh , Singh Harpreet , Mishra Arun Kumar , Chopra Shivani , Chopra Hitesh
Published: January 2025

Exosomes are small extracellular vesicles secreted by various cell types, playing a crucial role in intercellular
communication by carrying proteins, lipids, and nucleic acids, thus holding significant potential in
diagnostics and therapeutics. Accurate labeling of exosomes is vital for studying their biogenesis, trafficking,
and functional properties, enabling precise tracking and manipulation. This review examines current labeling
techniques, including metabolic glycan labeling, chemical tagging, membrane fluorescent dyes, bio-conjugation,
non-covalent labeling, and cell-engineering approaches. Each method is analyzed for its efficiency, specificity,
and practicality, with attention to potential artifacts and challenges. Advancements in these techniques
are essential for improving our understanding of exosome biology and developing exosome-based diagnostic
and therapeutic strategies, providing researchers with valuable insights into state-of-the-art techniques and
their applications in exosome research.
Therapeutic Potential of Menstrual Blood-Derived Stem Cells in Attenuating
Uterine Adhesion: Novel Strategies and Future Prospect
Authors: Rajabloo Yasamin , Al-Asady Abdulridha Mohammed , Avan Amir , Khazaei Majid , Ryzhikov Mikhail , Hassanian Seyed Mahdi
Published: January 2025

Intrauterine Adhesions (IUAs) are characterized by endometrial damage due to endometritis or
curettage. Currently, the gold standard for IUA treatment is hysteroscopy, which enables the dissolution of
IUA through mechanical or electrosurgical energy. Common strategies to prevent recurrence include the insertion of a balloon catheter or IUD in the uterus. Although hysteroscopy and postoperative strategies improve
the uterine cavity’s morphology and menstrual flow in some patients, infertility and adhesion recurrence rates
are among the problems that persist. Mesenchymal Stem Cells (MSCs) are ideal for tissue regeneration due to
their self-renewal and immunomodulatory characteristics. MSCs also exert anti-fibrotic properties in IUA treatment. However, the clinical application of stem cells is limited due to safety concerns and cost. In this review,
we have summarized the recent advances in the application of MSCs in IUA treatment.
Tris(pentafluorophenyl)borane [B(C6F5)3]-catalyzed Organic Transformations: A
Triennial Update (2021 Onwards)
Authors: Varala Ravi , Seema Vittal , Kamsali Murali Mohan Achari , Hussein Mohamed , Alam Mohammed Mujahid , Dubasi Narsimhaswamy
Published: January 2025

Although a number of Lewis acids based on boron have been identified thus far,
tris(pentafluorophenyl) borane [B(C6F5)3] (henceforth, BCF) has drawn the greatest interest
from the synthetic chemistry community. Due to its commercial availability and thermal
stability, BCF has been thoroughly investigated in organic and materials chemistry for its
potential as a Lewis acid catalyst. The last two decades have witnessed the majority of BCF
chemistry research in organic synthesis, and numerous novel catalytic reactivities are presently
being studied. In this triennial update, which covers the period from 2021 to the present,
we have thoroughly and critically reviewed the most representative applications of
tris(pentafluorophenyl)borane in organic synthesis. Mechanistic studies are presented together
with the scope of the catalyst.
Cellulose and Cellulose Nanomaterials: Recent Research and Applications in Medical Field
Authors: Khanday Murtaza Ahmad , Shergujri Danish Ahmad , Noor Aisha , Fayaz Shahid , Raza Syed Naiem , Mir Reyaz Hassan , Khan Nisar Ahmad
Published: January 2025

Cellulose, the most prevalent biopolymer in the world, is comprehensively reviewed.
Cellulose occurs in fibrillar patterns with alternating crystalline and amorphous regions. The
non-toxic and -friendly nature of cellulose has made it beneficial in many fields, such as pharmaceuticals,
biomedical, nanotechnology, etc. Numerous sources, including the plant cell wall
and wood, some types of bacteria, algae, and tunicates can provide cellulose. This polysaccharide
is composed of a straight chain of d-glucose units attached by β (1 → 4) that extend in number
from several hundred to thousands. This review details the recent progress in the synthesis of
cellulose nanofibers or nanofibrillated cellulose, cellulose nanocrystals, commonly referred to as
nanocellulose, and bacterial cellulose. The methodologies and procedures for evaluating the
morphology, purity, crystallinity, mechanical, rheological, and other characteristics of cellulose
micro/nanomaterials are covered in this review. The current study also discusses several ways to
functionalize CNFs, CNCs, and BC. Finally, a guide to the synthesis of cellulose nanocomposites
is provided, along with possible uses in the biomedical field, such as wound healing, bone
tissue engineering, and artificial blood vessel production. Additionally, applications related to
the pharmaceutical industry are also highlighted.
Mechanism of HJ11 Decoction in the Treatment of Atherosclerosis Based on Network Pharmacology and Experimental Validation
Authors: Lu Fei , Zou Jiaxi , Xu Weiming , Zhang Fangyuan , Nie Wenyi , Zhao Yue , Jiang Lijie , Liang Lizhe , Hu Jingqing
Published: January 2025

Background:

HJ11 (HJ11 decoction), which is based on the traditional prescription
Si-Miao-Yong-An decoction, has exerted a remarkable effect on atherosclerosis (AS).
Nevertheless, the main components and underlying mechanisms of HJ11 for treating AS remain
unclear.

Aim of the Study:

This study was designed to elucidate the mechanism of HJ11 in the treatment
of AS through network pharmacology and in vivo experimental validation.

Methods:

Network pharmacology was employed to explore the primary bioactive components
and targets of HJ11. AS-related genes were obtained from the GeneCards and DisGeNET databases
and screened for intersections with HJ11. A herb-compound-target interaction network
was constructed by Cytoscape 3.9.1, and molecular docking analyses were constructed on key
targets. By using a mouse model, the mechanism of action of HJ11 was further confirmed.

Results:

A total of 231 active components of HJ11, 1681 AS-related genes, and 156 common targets
were identified. Through the establishment of numerous networks, it was discovered that the
main association of the mechanism of HJ11 in AS therapy pertained to anti-inflammation. Important
substances included quercetin, kaempferol, and luteolin, while TNF-α, AKT1, IL-6, and
VEGFA were the main targets. Molecular docking demonstrated that there were favorable binding
interactions between active drugs (quercetin, kaempferol, and luteolin) and targets (TNF-α,
AKT1, IL-6, and VEGFA). In the in vivo study, HJ11 reduced the expression of TNF-α, AKT1,
IL-6, and VEGFA at both the mRNA and protein levels, inhibited atherosclerotic lesions in AS
mouse models, and retarded the development of retroarterioid sclerosis.

Conclusions:

HJ11 can inhibit inflammation and the progression of AS, and the mechanism
might involve downregulating the expression of TNF-α, AKT1, IL-6, and VEGFA.
The Effects of 1,8-Cineole Treatment on Benign Prostatic Hyperplasia in Rats
Authors: Al-Trad Bahaa , Abu Haija Yazan , Aljabali Alaa A. , Alomari Ghada , Tahat Lena , Al-Ghadi Muath Q.
Published: January 2025

Background:

Benign prostatic hyperplasia is a non-malignant growth of the prostate
gland; it’s the most common prostatic growth in aging men. 1,8-cineole is a natural compound
that is extracted from the essential oil of several aromatic plants including Eucalyptus spp. Recent
studies have demonstrated the anti-inflammatory, antioxidant, and anticancer activities of
1,8-cineole. This study aims to investigate the effects of 1,8-cineole treatment on the development
of BPH induced by testosterone in rats.

Method:

Thirty adult male rats were divided into three groups (n=10): a control group, an untreated
BPH group that received subcutaneous injections of testosterone (3 mg/kg), and a
BPH+cineole group that received 50 mg/kg of cineole intraperitoneally in addition to testosterone
for 21 days. Histological changes, serum testosterone, and dihydrotestosterone (DHT)
levels, prostatic tissue content of the inflammatory biomarkers interleukin 1 beta (IL-1β) and
tumor necrosis factor alpha (TNF-α), oxidative stress biomarkers superoxide dismutase (SOD)
and malondialdehyde (MDA), angiogenesis biomarker vascular endothelial growth factor
(VEGF-A), cellular proliferation biomarker proliferating cell nuclear antigen (PCNA), transforming
growth factor beta-1 (TGF-β1), and pro-apoptotic (Bax) and anti-apoptotic (Bcl-2)
genes were analyzed.

Results:

Cineole treatment led to a reduction in the prostate weight-to-body weight ratio, as well
as the restoration of histopathological changes caused by testosterone. Cineole treatment reduced
the serum levels of testosterone and DHT, and the prostatic tissue levels of IL-1β, TNF-α,
VEGF-A, PCNA, and TGF-β1 compared to those in the BPH group. Additionally, cineole treatment
enhanced the SOD activity and decreased the MDA levels in the prostate tissue. Finally,
the mRNA expression of the Bax was increased, while the expression of the Bcl-2 was decreased
by cineole.

Conclusion:

Our results highlight the effectiveness of cineole in preventing BPH development in
rats. This preventive effect was attributed to the regulation of inflammatory responses, oxidative
stress, cellular proliferation, and apoptosis.
Exploring Genetic and Receptor-Based Dopaminergic Strategies for Antidepressant Drug Development
Authors: Pannu Arzoo , Goyal Ramesh K.
Published: January 2025

The dopamine (DA) system is central to mood regulation, motivation, and reward processing, making it a critical focus for understanding Major Depressive Disorder (MDD). While the
dopaminergic system's role in MDD pathophysiology has been acknowledged, gaps remain in linking specific receptor subtypes and genetic factors to depression-like phenotypes. This study explores the interplay between dopamine receptor subtypes (D1-D5) and associated genetic variations,
particularly focusing on receptor heterodimers and polymorphisms influencing dopamine biosynthesis, signalling, and metabolism. A comprehensive review of molecular mechanisms highlights
key findings: alterations in D1-D2 heterodimers contribute to mood dysregulation; D3 receptor
downregulation correlates with depressive behaviour; and genetic polymorphisms, including those
in tyrosine hydroxylase and dopamine transporter (DAT) genes, influence dopamine levels and receptor functions. Emerging data from neuroimaging and animal models confirm the pivotal role of
dopamine receptor subtypes in MDD, offering insights into their therapeutic targeting. Here, we
show that dopaminergic dysfunction underpins MDD's pathophysiology, with receptor-specific
mechanisms presenting novel drug targets. Understanding these pathways facilitates precision medicine approaches, bridging the gap between genetic predisposition and receptor pharmacology, and
paving the way for tailored antidepressant strategies with improved efficacy and reduced side effects.
Preventive and Therapeutic effects of Metformin in Cancer: A Meta-Analysis of RCT and Cohort Studies
Authors: Getu Mikiyas Amare , Zhang Xianbin , Wang Jia , Xu Kunpeng , Ying Ying , Ma Li , Gong Peng
Published: January 2025

Background:

There is discrepancy of results among various individual and me-ta-analytical studies about the effect of metformin on cancer risk and patients’ survival. Therefore, we have conducted a comprehensive, updated meta-analysis to evaluate the preventive and therapeutic effects of metformin for cancer patients, as well as the inci-dence of adverse reactions, among metformin users.

Methods:

A total of 18 studies (10 cohort studies and 8 randomized controlled trials) in-volving 1,300,820 participants from Europe, North America, and Asia were included in this meta-analysis.

Results:

No significant association was found between metformin use and overall survival (Hazard ratio = 1.02; 95% CI, 0.80 – 1.30) and progressive-free
survival (Hazard ratio = 1.00; 95% CI, 0.76 – 1.31). In addition, the summary risk estimates for adverse reactions of metformin were not statistically
significant between intervention and control groups (Risk Ratio = 1.11; 95% CI, 0.94 – 1.31). However, metformin use was associated with a reduction in
cancer risk (Hazard ratio = 0.90, 95% CI, 0.86 – 0.94).

Conclusion:

Metformin does not significantly prolong the overall survival and progressive-free survival of cancer patients, but it may effectively reduce the
risk of cancer occurrence.
PAV-05 Naphthoquinone Potently Inhibit Zika Virus Replication in Infected Cells
Authors: Cesar Cirne-Santos Claudio , Gomes Gonzaga Daniel Tadeu , Oliveira de Resende Gabriel , Gonçalves Mariana de Castro , Andrade Aymee da Silva , Furtado Pacheco Paulo Anastácio , dos Santos-Rodrigues Alexandre , Rabelo Vitor Won-Held , Abreu Paula Alvarez , Faria Robson Xavier , da Rocha David Rodrigues , da Silva Fernando de Carvalho , Ferreira Vitor Francisco , de Souza Barros Caroline , Nunes de Palmer Paixão Izabel Christina
Published: January 2025

Background:

Zika (ZIKV) is a virus transmitted by mosquitoes that can cause Guillain-
Barré syndrome and congenital malformations like microcephaly. Given its explosive resurgence
and the resulting epidemics in 2016, the search for effective antiviral drugs has become absolutely
necessary.

Methods:

In this study, we examined the potential of naphthoquinone derivatives that have a sulfonamide
or sulfonate group to inhibit ZIKV replication in primary cultured neurons and in Vero cells.

Results:

In our in vitro studies, we found that PAV05 had low cytotoxicity with a CC50 of 329
μM ±3.6 for Vero cells and 290 μM ±3.5 for neurons. Additionally, we observed a strong inhibitory
activity on viral replication with an EC50 value of EC50 of 0.92 μM ±0.15 in Vero cells, resulting
in a Selectivity Index (SI) of 357. Even when added 16 hours post-infection, PAV05 maintained
its inhibitory effect. When PAV05 was evaluated in sub-optimal concentrations together
with Ribavirin, we observed a strong synergistic effect, with an inhibition greater than 90% even at
doses of 0.5 μM. In silico tests suggested that PAV05 may have effects on ZIKV NS2B-NS3.

Conclusion:

The ZIKV inhibitor described in this study shows promise as a compound for the
development of therapies against ZIKV. It may also be considered for inclusion in the portfolio of
broad-spectrum antiflavivirus inhibitors.

conclusion:

The ZIKV inhibitor described in this study shows promise as a potential compound for the development of anti-ZIKV therapies. It may also be considered for inclusion in the portfolio of broad-spectrum antiflavivirus drugs.