bluuebunny/crossref_metadata_2025 huggingface dataset:
An improved U-Net-based in situ root system phenotype segmentation method for plants
Authors: Li Yuan , Huang Yunlian , Wang Mengxue , Zhao Yafeng
Published: March 2023

The condition of plant root systems plays an important role in plant growth and development. The Minirhizotron method is an important tool to detect the dynamic growth and development of plant root systems. Currently, most researchers use manual methods or software to segment the root system for analysis and study. This method is time-consuming and requires a high level of operation. The complex background and variable environment in soils make traditional automated root system segmentation methods difficult to implement. Inspired by deep learning in medical imaging, which is used to segment pathological regions to help determine diseases, we propose a deep learning method for the root segmentation task. U-Net is chosen as the basis, and the encoder layer is replaced by the ResNet Block, which can reduce the training volume of the model and improve the feature utilization capability; the PSA module is added to the up-sampling part of U-Net to improve the segmentation accuracy of the object through multi-scale features and attention fusion; a new loss function is used to avoid the extreme imbalance and data imbalance problems of backgrounds such as root system and soil. After experimental comparison and analysis, the improved network demonstrates better performance. In the test set of the peanut root segmentation task, a pixel accuracy of 0.9917 and Intersection Over Union of 0.9548 were achieved, with an F1-score of 95.10. Finally, we used the Transfer Learning approach to conduct segmentation experiments on the corn in situ root system dataset. The experiments show that the improved network has a good learning effect and transferability.
Genome-wide identification of NHX (Na+/H+ antiporter) gene family in Cucurbita L. and functional analysis of CmoNHX1 under salt stress
Authors: Shen Changwei , Yuan Jingping , Li Xin , Chen Ruixiang , Li Daohan , Wang Fei , Liu Xing , Li Xinzheng
Published: March 2023

Soil salinization, which is the accumulation of salt in soil, can have a negative impact on crop growth and development by creating an osmotic stress that can reduce water uptake and cause ion toxicity. The NHX gene family plays an important role in plant response to salt stress by encoding for Na+/H+ antiporters that help regulate the transport of sodium ions across cellular membranes. In this study, we identified 26 NHX genes in three cultivars of Cucurbita L., including 9 Cucurbita moschata NHXs (CmoNHX1-CmoNHX9), 9 Cucurbita maxima NHXs (CmaNHX1-CmaNHX9) and 8 Cucurbita pepo NHXs (CpNHX1-CpNHX8). The evolutionary tree splits the 21 NHX genes into three subfamilies: the endosome (Endo) subfamily, the plasma membrane (PM) subfamily, and the vacuole (Vac) subfamily. All the NHX genes were irregularly distributed throughout the 21 chromosomes. 26 NHXs were examined for conserved motifs and intron-exon organization. These findings suggested that the genes in the same subfamily may have similar functions while genes in other subfamilies may have functional diversity. The circular phylogenetic tree and collinearity analysis of multi-species revealed that Cucurbita L. had a substantially greater homology relationship than Populus trichocarpa and Arabidopsis thaliana in terms of NHX gene homology. We initially examined the cis-acting elements of the 26 NHXs in order to investigate how they responded to salt stress. We discovered that the CmoNHX1, CmaNHX1, CpNHX1, CmoNHX5, CmaNHX5, and CpNHX5 all had numerous ABRE and G-box cis-acting elements that were important to salt stress. Previous transcriptome data showed that in the mesophyll and veins of leaves, many CmoNHXs and CmaNHXs, such as CmoNHX1, responded significantly to salt stress. In addition, we heterologously expressed in A. thaliana plants in order to further confirm the response of CmoNHX1 to salt stress. The findings demonstrated that during salt stress, A. thaliana that had CmoNHX1 heterologously expression was found to have decreased salt tolerance. This study offers important details that will aid in further elucidating the molecular mechanism of NHX under salt stress.
The relationship between self-regulated learning, mindful agency, and psychological resilience in Chinese master of nursing specialists: A cross-sectional study
Authors: Yang Rui , Gao YuFang , Ji ZiTong
Published: March 2023

Background

Self-regulated learning helps to improve academic performance and is an important strategy for the sustainable development of Master of Nursing Specialists. Consequently, it is relevant to identify the factors that affect self-regulated learning and analyze the correlation between them.

Objective

This study examined the status quo of self-regulated learning, the relationship between self-regulated learning, mindful agency, and psychological resilience, and explored whether mindful agency and psychological resilience influence self-regulation learning.

Methods

Chinese Master of Nursing Specialists were recruited to participate in an online survey from March to November 2022. Self-regulated learning, mindful agency, and psychological resilience were measured using three questionnaires, including the Self-Regulated Learning Scale for Clinical Nursing Practice Scale (SRLS-CNP), the Mindful Agency Scale, and the 10-item Connor-Davidson Resilience Scale (CD-RISC-10). The data were processed and analyzed using SPSS26.0. The statistical methods included descriptive statistics, Pearson’s correlation analyses, and multiple linear regression.

Results

Self-regulated learning of Chinese Master of Nursing Specialists was medium level (59.24 ± 9.33 scores). Mindful agency and psychological resilience were positively correlated with self-regulated learning (p < 0.01) and important predictors of self-regulated learning of Master of Nursing Specialists, explaining 44.6% of the variation.

Conclusion

Mindful agency and psychological resilience affected the self-regulated learning level of Master of Nursing Specialists in clinical practice. These results will enable clinical educators to pay more attention to the personal psychological factors of Master of Nursing Specialists to improve their self-regulated learning ability through mindful agency and psychological resilience.
Grievance-fueled sexual violence
Authors: Higgs Tamsin , Darjee Rajan , Davis Michael R. , Carter Adam J.
Published: March 2023

The grievance fueled violence paradigm encompasses various forms of targeted violence but has not yet been extended to the theoretical discussion of sexual violence. In this article, we argue that a wide range of sexual offenses can be usefully conceptualized as forms of grievance fueled violence. Indeed, our assertion that sexual violence is often grievance fueled is unoriginal. More than 40 years of sexual offending research has discussed the pseudosexual nature of much sexual offending, and themes of anger, power, and control – themes that draw clear parallels to the grievance fueled violence paradigm. Therefore, we consider the opportunities for theoretical and practical advancement through the merging of ideas and concepts from the two fields. We examine the scope of grievance in the context of understanding sexual violence, and we look to the role of grievance in the trajectory toward both sexual and nonsexual violence, as well as factors that might distinguish grievance fueled sexual from nonsexual violence. Finally, we discuss future research directions and make recommendations for clinical practice. Specifically, we suggest that grievance represents a promising treatment target where risk is identified for both sexual and nonsexual violence.
Effect of short-term mindfulness-based stress reduction on sleep quality in male patients with alcohol use disorder
Authors: Wang Yongmei , Chen Cuiping , Gu Lina , Zhai Yi , Sun Yanhong , Gao Guoqing , Xu Yayun , Pang Liangjun , Xu Lianyin
Published: March 2023

Background

Sleep disturbance is one of the most prominent complaints of patients with alcohol use disorder (AUD), with more than 70% of patients with AUD reporting an inability to resolve sleep problems during abstinence. Mindfulness-based stress reduction (MBSR) has been shown to improve sleep quality and as an alternative therapy to hypnotics for sleep disorders.

Objective

The aim of the present study was to evaluate the effect of short-term MBSR on sleep quality in male patients with AUD after withdrawal.

Methods

A total of 91 male patients with AUD after 2 weeks of routine withdrawal therapy were randomly divided into two groups using a coin toss: the treatment group (n = 50) and the control group (n = 41). The control group was received supportive therapy, and the intervention group added with MBSR for 2 weeks on the basis of supportive therapy. Objective sleep quality was measured at baseline and 2 weeks after treatment using the cardiopulmonary coupling (CPC). Indicators related to sleep quality include total sleep time, stable sleep time, unstable sleep time, rapid eye movement (REM) sleep time, wake-up time, stable sleep latency, sleep efficiency, and apnea index. These indicators were compared by an analysis of covariance (ANCOVA) between the two groups, controlling for individual differences in the respective measures at baseline.

Results

The results showed that there were no significant differences in the age [t (89) = –0.541, P = 0.590), BMI [t (89) = –0.925, P = 0.357], educational status [t (89) = 1.802, P = 0.076], years of drinking [t (89) = –0.472, P = 0.638), daily intake [t (89) = 0.892, P = 0.376], types of alcohol [χ2 (1) = 0.071, P = 0.789], scores of CIWA-AR [t (89) = 0.595, P = 0.554], scores of SDS [t (89) = –1.151, P = 0.253), or scores of SAS [t (89) = –1.209, P = 0.230] between the two groups. Moreover, compared with the control group, the total sleep time [F (1.88) = 4.788, P = 0.031) and stable sleep time [F (1.88) = 6.975, P = 0.010] were significantly increased in the treatment group. Furthermore, the average apnea index in the patients who received MBSR was significantly decreased than in the control group [F (1.88) = 5.284, P = 0.024].

Conclusion

These results suggest that short-term MBSR could improve sleep quality and may serve as an alternative treatment to hypnotics for sleep disturbance in patients with AUD after withdrawal.
Epidemiological investigation of norovirus infections in Punjab, Pakistan, through the One Health approach
Authors: Yasir Ammar , Mahmood Yasir , Yaqoob Muhammad Arsalan , Zia Ubaid-ur-Rehman , Munoz-Zanzi Claudia , Alam Muhammad Masroor , Warraich Muhammad Akib , Hassan Mushtaq Muhammad
Published: March 2023

Introduction

Norovirus, mainly associated with acute gastroenteritis, is very contagious and can affect a vast range of species ranging from cattle, pigs, dogs, mice, cats, sheep, and lions to humans. It is a foodborne pathogen that mainly transmits through the fecal–oral route.

Methods

This is the first-ever study conducted in Lahore and Sheikhupura districts of Punjab, Pakistan, to investigate noroviruses through the One Health approach. From January 2020 to September 2021, 200 fecal samples were collected from clinical cases of hospitalized patients and 200 fecal samples from sick animals at veterinary hospitals and local farms. In addition, 500 food and beverage samples were collected from street vendors and retail stores. A predesigned questionnaire was used to assess the risk factors and clinical characteristics of sick people and animals.

Results and discussion

Overall, 14% of the human clinical samples were positive by RT-PCR for genogroup GII. All bovine samples were negative. Food and beverage samples were tested in pools, resulting in sugarcane juice samples positive for genogroup GII. Previous contact with acute gastroenteritis patients, sex, and presence of vomiting were found to be significant risk factors (p ≤ 0.05). The substantial number of diarrhea cases associated with noroviruses calls for additional studies to investigate the epidemiology and transmission and to improve surveillance.
Incidence and risk factors of hearing loss in patients with Turner Syndrome
Authors: Lin Huijia , Wang Xiaoya , Qin Shuang , Luo Fanglan , Cen Yingmei , Lash Gendie E. , Li Li
Published: March 2023

Background

Hearing loss (HL) is one of the main medical complications for Turner Syndrome (TS) patients, with an earlier presentation and higher incidence than normal women. However, the etiology of HL in TS is unclear. The aim of this study was to investigate the hearing status of TS patients in China and the influencing factors, so as to provide a theoretical basis for early intervention treatment for TS patients with HL.

Methods

In total 46 female patients aged 14–32 diagnosed with TS received tympanic membrane and audiological examinations, including pure tone audiometry and tympanometry. In addition, the effects of karyotype, sex hormone levels, thyroid function, insulin, blood lipids, bone mineral density, age and other factors on hearing levels were analyzed, and the possible risk factors associated with HL in TS patients were explored.

Results

In 9 patients (19.6%) had HL, including 1 (2.2%) with mild conductive hearing loss, 5 (10.9%) with mild sensorineural hearing loss, 3 (6.5%) with moderate sensorineural hearing loss. TS is often associated with age-related mid-frequency and high-frequency HL, and the incidence of HL increases with age. Compared with other karyotypes, patients with 45, X haplotype have an increased risk of mid-frequency HL.

Conclusions

Therefore, karyotype may be a predictor of hearing problems in TS.
COVID-19 case prediction using emotion trends via Twitter emoji analysis: A case study in Japan
Authors: Tran Vu , Matsui Tomoko
Published: March 2023

Introduction

The worldwide COVID-19 pandemic, which began in December 2019 and has lasted for almost 3 years now, has undergone many changes and has changed public perceptions and attitudes. Various systems for predicting the progression of the pandemic have been developed to help assess the risk of COVID-19 spreading. In a case study in Japan, we attempt to determine whether the trend of emotions toward COVID-19 expressed on social media, specifically Twitter, can be used to enhance COVID-19 case prediction system performance.

Methods

We use emoji as a proxy to shallowly capture the trend in emotion expression on Twitter. Two aspects of emoji are studied: the surface trend in emoji usage by using the tweet count and the structural interaction of emoji by using an anomalous score.

Results

Our experimental results show that utilizing emoji improved system performance in the majority of evaluations.
COVID-Twitter-BERT: A natural language processing model to analyse COVID-19 content on Twitter
Authors: Müller Martin , Salathé Marcel , Kummervold Per E.
Published: March 2023

Introduction

This study presents COVID-Twitter-BERT (CT-BERT), a transformer-based model that is pre-trained on a large corpus of COVID-19 related Twitter messages. CT-BERT is specifically designed to be used on COVID-19 content, particularly from social media, and can be utilized for various natural language processing tasks such as classification, question-answering, and chatbots. This paper aims to evaluate the performance of CT-BERT on different classification datasets and compare it with BERT-LARGE, its base model.

Methods

The study utilizes CT-BERT, which is pre-trained on a large corpus of COVID-19 related Twitter messages. The authors evaluated the performance of CT-BERT on five different classification datasets, including one in the target domain. The model's performance is compared to its base model, BERT-LARGE, to measure the marginal improvement. The authors also provide detailed information on the training process and the technical specifications of the model.

Results

The results indicate that CT-BERT outperforms BERT-LARGE with a marginal improvement of 10-30% on all five classification datasets. The largest improvements are observed in the target domain. The authors provide detailed performance metrics and discuss the significance of these results.

Discussion

The study demonstrates the potential of pre-trained transformer models, such as CT-BERT, for COVID-19 related natural language processing tasks. The results indicate that CT-BERT can improve the classification performance on COVID-19 related content, especially on social media. These findings have important implications for various applications, such as monitoring public sentiment and developing chatbots to provide COVID-19 related information. The study also highlights the importance of using domain-specific pre-trained models for specific natural language processing tasks. Overall, this work provides a valuable contribution to the development of COVID-19 related NLP models.
A multi-spark electrohydraulic shock wave generator with adjustable pressure field distribution and beam steering capability
Authors: Sankin Georgy N. , Fang Zheng , Gu Juanjuan , Jing Yun , Zhong Pei
Published: March 2023

Background and objective

All clinical shock wave lithotripters produce an axisymmetric acoustic field without accounting for the anatomic features of the kidney or respiratory motion of the patient. This work presents a steerable and adjustable focusing electrohydraulic (SAFE) shock wave generator design with variable beam size and shape.

Materials and methods

90 electrohydraulic transducers are mounted concentrically on a spherical basin with adjustable connection to individual transducers. Each transducer consists of 45 3D-printed titanium microelectrodes embedded in epoxy with a tip diameter of 0.3 mm. All the transducers are arranged in 5 concentric rings and sub-divided into 6 sectors.

Results

By changing the connections of individual transducers, the focused pressure field produced by the transducer array can be either axisymmetric with a -6 dB focal width of 14.8 mm in diameter, or non-axisymmetric with a long axis of 22.7 mm and a short axis of 15.1 mm. The elongated beam produces a peak positive pressure of 33.7 ± 4.1 MPa and comminution efficiency of 42.2 ± 3.5%, compared to 36.2 ± 0.7 MPa and 28.6 ± 6.1% for axisymmetric beam after 150 pulses at 20 kV.

Conclusions

We have demonstrated that the SAFE shock wave generator can produce an elongated non-axisymmetric pressure field with higher stone comminution efficiency. The SAFE shock wave generator may provide a flexible and versatile design to achieve accurate, stable, and safe lithotripsy for kidney stone treatment.
The Light and Sound Quiz Show
Authors: Memoli Gianluca
Published: March 2023

The science of light manipulation started with the ancient Greeks, so we have had many years to develop it. Lenses and holograms are part of our everyday lives. Light and sound are very similar: they are both waves, and they both have particles associated with them. So, why do we not have lenses or displays for sound? Or do we? This article will tell the story of how sound technology is catching up with light technology. We will tell you about acoustic metamaterials, an emerging technology that is quickly becoming part of our loudspeakers, our shows, our cars, our public spaces, and our hospitals—all the places where we want control over sound and noise. The future of shaping and designing sound is in the making! Maybe someday, sound experts will even teach something to light experts!
Do crop diversity and livestock production improve smallholder intra-household dietary diversity, nutrition and sustainable food production? Empirical evidence from Pakistan
Authors: Waseem Muhammad , Li Xiaoyun , Jamil Ihsan , Islam Abu Hayat Md. Saiful , Abbas Qasir , Raza Muhammad Haseeb , Eliw Moataz
Published: March 2023

Crop diversification and livestock production is an important strategy to enhance nutrition, sustainable food production, and improve food security, especially at the smallholder household level. However, existing evidences are mixed and there are limited information about the relationship among agriculture crops, household income diversification (HID), and household dietary diversity (HDD) among smallholder farmers in developing country setting like Pakistan. Therefore, this study aims to understand the role of crop diversification (CD) on HDD, nutrition, and sustainable agriculture and food production in the context of smallholder households in Punjab, Pakistan. The study employed ordered probit regression and cross-sectional data from 450 households collected using 24-h recall method. Regression results indicate that crop diversity and intra-household dietary diversity are positively associated across adults, adolescents, and children in all the study districts. Moreover, annual income, key crops grown by the household and family education are also the significant drivers of dietary diversity. Greater travel distance between markets was the most crucial factor in all regions which significantly affect dietary diversity. The overall research findings indicated that crop diversification and livestock production in the selected areas is significantly contributing to improve nutrition and sustainable food production. Therefore this study recommends for promoting crop diversification and livestock production for sustainable agricultural development and improving nutrition in the context of developing countries like Pakistan.
Expression of hypoxia-inducing factor-1α and matrix metalloproteinase-9 in the recipient parasylvian cortical arteries with different hemodynamic sources in adult moyamoya disease
Authors: Luo Mingrui , Yu Jin , Xin Can , Hu Miao , Tao Tianshu , Wan Guiping , Chen Jincao , Zhang Jianjian
Published: March 2023

Objective

In our latest research, we have demonstrated that the recipient parasylvian cortical arteries (PSCAs) with hemodynamic sources from the middle cerebral artery (M-PSCAs) has a higher risk of postoperative cerebral hyperperfusion (CHP) syndrome than those from non-M-PSCAs in adult moyamoya disease (MMD) patient. However, whether there are differences between M-PSCAs and non-M-PSCAs in vascular specimens characteristics has not been studied. In this study, we further investigate the vascular specimen of recipient PSCAs by histological and immunohistochemical methods.

Methods

50 vascular specimens of recipient PSCAs were obtained from 50 adult MMD patients during the combined bypass surgeries in our departments of Zhongnan hospital. 4 recipient PSCAs samples were also obtained in the same way from the middle cerebral artery occlusion patients. The samples were received the pathological sectioning, hematoxylin and eosin staining, and immunohistochemistry, then the vascular wall thickness, matrix metalloproteinase-9 (MMP-9) and hypoxia-inducing factor-1α (HIF-1α) were analyzed.

Results

M-PSCAs adult MMD patients had a thinner intima than non-M-PSCAs in the recipient PSCAs specimens. In recipient non-M-PSCAs vascular specimens, the immunoreactivity indicating HIF-1α and matrix metalloproteinase-9 (MMP-9) was significantly higher than M-PSCAs groups. The logistic regression analyses showed that the M-PSCAs was an independent risk factor of postoperative cerebral hyperperfusion (CHP) syndrome (OR 6.235, 95% CI1.018-38.170, P = 0.048) in MMD.

Conclusion

Our results indicate that M-PSCAs adult MMD patients had thinner intima than non-MCAs adult MMD patients in the PSCAs. More importantly, HIF-1α and MMP-9 were overexpressed in non-M-PSCAs vascular specimens.
Outcomes of direct superior approach and posterolateral approach for hemiarthroplasty in the treatment of elderly patients with displaced femoral neck fractures: A comparative study
Authors: Hu Wei , Xu Wen-Bo , Li Hao , Jiang Wen-Hua , Shao Yin-Chu , Shan Ji-Chun , Yang Di , Wan De-En , Shuang Feng
Published: March 2023

Hemiarthroplasty is a surgical choice for super-aged patients with a high surgical risk and a sedentary lifestyle. The direct superior approach (DSA), a minimally invasive modification of the posterior approach, is rarely studied in hemiarthroplasty. The aim of the present study was to compare the clinical outcomes in elderly patients with displaced femoral neck fractures undergoing hemiarthroplasty via DSA with the conventional posterolateral approach (PLA). A total of 48 elderly patients with displaced femoral neck fractures who underwent hemiarthroplasty between February 2020 and March 2021 were retrospectively included in the study. Of them, 24 patients (mean age 84.54 ± 2.11 years) were treated with hemiarthroplasty via DSA (DSA group), while the other 24 patients (mean age 84.92 ± 2.15 years) were treated with hemiarthroplasty via PLA (PLA group). Clinical outcomes, perioperative data, and complications were recorded. There were no obvious differences in the baseline characteristics between the DSA and PLA groups, including age, gender, body mass index, Garden type, American Society of Anesthesiologists score, and hematocrit. Perioperative data showed that the length of the incision in the DSA group was smaller than that in the PLA group (p < 0.001). However, the duration of the operation and blood loss in the DSA group were longer and higher than those in the PLA group, respectively (p < 0.001). In addition, the DSA group had a shorter hospitalization time than the PLA group (p < 0.001). The visual analog scale score and Harris score 1 month postoperatively in the DSA group were better than those in the PLA group (p < 0.001). Moreover, there were no significant differences between the two groups in Harris score (for assessment dysfunction) 6 months postoperatively (p > 0.05). DSA is less invasive and has better clinical outcomes, which can allow an early return to daily living activities in elderly patients with displaced femoral neck fractures undergoing hemiarthroplasty.
Long-term nutritional management of an obese German Spitz with paroxysmal dyskinesia, calcium oxalate urolithiasis, and suspected pancreatitis—A case report
Authors: Baptista da Silva Camila , Hermans Michelle , Ruiz-Suárez Norberto , Verdoodt Fien , Bhatti Sofie Fatima Mareyam , Hesta Myriam
Published: March 2023

Background

To our knowledge, this is the first description of long-term nutritional management in a dog with paroxysmal dyskinesia.

Case summary

An obese 9-year-old, male entire, German Spitz was presented for dietary management after being diagnosed with calcium oxalate urolithiasis and suspected pancreatitis. Since he was seven years old, the dog has had a history of neurological signs, which were thought to be epileptic seizures. He was treated with phenobarbital and potassium bromide and was clinically controlled. For his nutritional advice, aiming to minimize one of the most important risk factors for the diseases, a weight loss program was started and successfully executed. However, 10 months later, the dog restarted presenting neurological episodes at a high frequency (3x/week). Based on videos and the characteristics of the neurological signs, the dog was diagnosed with paroxysmal dyskinesia. To investigate the role of gluten intake on this patient's neurological signs, a dietary trial with a commercial hypoallergenic diet (gluten-free; hydrolyzed protein) was followed. During the 3 months of the dietary trial, four neurologic episodes related to food indiscretion occurred. Upon the decrease in neurological episodes, the anti-seizure drugs were slowly discontinued. During this period, the dog presented only two neurologic episodes that were related to the days that the anti-seizure drugs were decreased. For 4 months the dog remained episode-free. However, a change in the dog's diet to another gluten-free diet (higher fat) led the dog to vomit and experience another neurologic episode. Once the dog was back to the previous gluten-free diet, it clinically improved, and no other clinical signs were reported by the client during the next 5 months.

Conclusion

Although a relationship between gluten and paroxysmal dyskinesia cannot be confirmed, the dog's improvement after the nutritional management and the removal of the anti-seizure therapy is supportive of dietary association.
Characterization and pathogenicity evaluation of recombinant novel duck reovirus isolated from Southeast China
Authors: Yang Huihu , Zhang Wandi , Wang Meihong , Yuan Sheng , Zhang Xuelian , Wen Feng , Guo Jinyue , Mei Kun , Huang Shujian , Li Zhili
Published: March 2023

The novel duck reovirus (NDRV) emerged in southeast China in 2005. The virus causes severe liver and spleen hemorrhage and necrosis in various duck species, bringing serious harm to waterfowl farming. In this study, three strains of NDRV designated as NDRV-ZSS-FJ20, NDRV-LRS-GD20, and NDRV-FJ19 were isolated from diseased Muscovy ducks in Guangdong and Fujian provinces. Pairwise sequence comparisons revealed that the three strains were closely related to NDRV, with nucleotide sequence identities for 10 genomic fragments ranging between 84.8 and 99.8%. In contrast, the nucleotide sequences of the three strains were only 38.9–80.9% similar to the chicken-origin reovirus and only 37.6–98.9% similar to the classical waterfowl-origin reovirus. Similarly, phylogenetic analysis revealed that the three strains clustered together with NDRV and were significantly different from classical waterfowl-origin reovirus and chicken-origin reovirus. In addition, the analyses showed that the L1 segment of the NDRV-FJ19 strain was a recombinant of 03G and J18 strains. Experimental reproduction of the disease showed that the NDRV-FJ19 strain was pathogenic to both ducks and chickens and could lead to symptoms of hemorrhage and necrosis in the liver and spleen. This was somewhat different from previous reports that NDRV is less pathogenic to chickens. In conclusion, we speculated that the NDRV-FJ19 causing duck liver and spleen necrosis is a new variant of a duck orthoreovirus that is significantly different in pathogenicity from any previously reported waterfowl-origin orthoreovirus.
Design of Double-Roller Anisotropic Force-Feeding Device for the D200 Single Screw Straw Fiber Extruder
Authors: Li Longhai , Zhao Chengshun , Gao Chuang , Fan Siyuan , Wang Xing , Chen Haitao , Ji Wenyi
Published: March 2023

A force-feeding device with a double-roller anisotropic was designed for the D200 single screw straw fiber extruder to keep the performance continuity of the system, which could improve the productivity of straw fiber. Four factors (the diameter of the auxiliary roll, the difference in linear speed of the two rolls, the gap between two rollers and the spindle speed as the test factors) were investigated to establish regression model to analyze the influence of the coupling of multiple factors on the test indexes. It was demonstrated that significant effects (p < 0.05) on the feeding rate and material loss were produced by four factors according to the experimental results. The sequence of influence in descending order on feeding rate was spindle speed, linear speed difference, gap and auxiliary roller diameter. The effects on materials loss in descending order were auxiliary roller diameter, line speed difference, spindle speed and gap. The optimal combination of parameters was obtained by the response surface, which were an auxiliary roller diameter of 230 mm, a spindle speed of 104.49 rpm, a line speed difference of 2840 mm/s and a gap between the two rolls of 14 mm. The average feeding rate was 2.3798 t/h, and the loss was 1.908 kg/h, and the errors were within 3.28%, which satisfied the feeding requirements for rice straw fiber production with high feeding efficiency and low raw material losses. This study provided a reference for the forced compression feeding and fibrillation process of rice straw.
Influence of Calcium on the Development of Corn Plants Grown in Hydroponics
Authors: do Moraes Gatti Victória Carolline , da Silva Barata Henrique , Silva Vicente Filho Alves , da Cunha Fernando França , de Oliveira Rubens Alves , de Oliveira Job Teixeira , Silva Priscilla Andrade
Published: March 2023

This work aimed to evaluate the effect of calcium on the development of corn plants grown with the omission and excess of calcium in a nutrient solution. The experiment was conducted in a greenhouse from March to May 2012. Three concentrations of calcium (0, 200, and 600 mg Ca L−1) were added to the nutrient solution, which was renewed weekly, for a total of 40 days. The following variables were measured weekly: the number of leaves, average stem diameter, dry weight of the plant shoots and roots, and visual leaf diagnosis. The results showed that when the plants were deprived of calcium, their root systems were significantly reduced, as determined by the Tukey test (p ≤ 0.05). The plants with calcium deprivation had shorter roots and a dark brown color and displayed initial symptoms of chlorosis in their young leaves, which eventually led to necrosis and tipping. Hydroponics is promising and has shown satisfactory production results, contributing to the improvement of the environment, job creation, and increased profit for rural producers.
Manipulation of CBTS1 Expression Alters Tobacco Resistance to Spodoptera frugiperda and Phytophthora nicotianae
Authors: Guan Jian , Du Zaifeng , Tian Tian , Wang Wenjing , Ju Fuzhu , Lin Xiaoyang , Zhang Zhongfeng , Cao Yi , Zhang Hongbo
Published: March 2023

Cembranoids produced by tobacco glandular trichomes have bioactivities in resistance to insect pests and pathogens. Cembratrien-ol synthase (CBTS) plays a key role in the biosynthesis of cembranoids and directly determines the cembranoid content in tobacco. This study examined the effect of changing CBTS1 expression on tobacco resistance to the insect pest Spodoptera frugiperda and oomycete pathogen Phytophthora nicotianae. The CDS sequence of CBTS1 was cloned into gene overexpression and silencing vectors and introduced into tobacco (Nicotiana tabacum L. cv. TN90) to obtain CBTS1-overexpression plants (CBTS1-OE) and CBTS1-silenced plants (CBTS1-RI). Compared with control plants, the content of cembratrien-ol (CBT-ol) was increased 4.48 times in the CBTS1-OE plants but decreased by 68% in the CBTS1-RI plants, while that of cembratrien-diol (CBT-diol) was increased 3.17 times in the CBTS1-OE plants but decreased by 76% in the CBTS1-RI plants. The S. frugiperda resistance of transgenic tobacco plants was evaluated by in vitro toxicity test, and the results showed that the resistance of CBTS1-OE plants to S. frugiperda was significantly improved but that of CBTS1-RI plants was reduced. The P. nicotianae resistance of transgenic tobacco plants was assessed by the detached leaf assay, and the results showed that the resistance of CBTS1-OE plants to P. nicotianae was enhanced, while that of CBTS1-RI plants was attenuated. Further gene expression analysis showed that overexpression of CBTS1 increased the expression of the pathogen-related gene PR-1a, while silencing of CBTS1 decreased its expression. This study demonstrated that manipulating the expression of CBTS1 could change the cembranoid content in tobacco plants and alter their resistance to both insect pests and oomycete pathogens.
Milk Fat Globule Membrane Relieves Fatigue via Regulation of Oxidative Stress and Gut Microbiota in BALB/c Mice
Authors: Zou Xiaoxiao , Yokoyama Wallace , Liu Xiaohui , Wang Kai , Hong Hui , Luo Yongkang , Tan Yuqing
Published: March 2023

Milk fat globule membranes (MFGMs) are complex structures that incorporate bioactive proteins and lipids to assist in infant development. However, the antifatigue and antioxidant potentials of MFGM have not been investigated. In this study, repeated force swimming measured fatigue in male BALB/c mice fed MFGM and saline for 18 weeks. The MFGM supplementation increased the time to exhaustion by 42.7% at 6 weeks and 30.6% at 14 weeks (p < 0.05). Fatigue and injury-related biomarkers, including blood glucose, lactic acid, and lactate dehydrogenase, were ameliorated after free swimming (p < 0.05). The activity of antioxidant enzymes in blood serum increased at 18 weeks, while malondialdehyde (MDA) content decreased by 45.0% after the MFGM supplementation (p < 0.05). The Pearson correlation analysis showed a high correlation between fatigue-related indices and antioxidant levels. The increased protein expression of hepatic Nrf2 reduced the protein expression of Caspase-3 in the gastrocnemius muscle (p < 0.05). Moreover, the MFGM supplementation increased the relative abundance of Bacteroides, Butyricimonas, and Anaerostipes. Our results demonstrate that MFGM may maintain redox homeostasis to relieve fatigue, suggesting the potential application of MFGM as an antifatigue and antioxidant dietary supplement.
Biological Evaluation and Structural Analysis of Some Aminodiphenylamine Derivatives
Authors: Bujor Alexandru , Hanganu Anamaria , Tecuceanu Victorita , Madalan Augustin , Tudose Madalina , Marutescu Luminita , Popa Marcela , Chifiriuc Carmen , Zarafu Irina , Ionita Petre
Published: March 2023

4-Aminodiphenylamino derivatives were investigated for their antioxidant and hydrophobicity character, together with other biological measurements, such as antimicrobial and antibiofilm activity. Among these nine compounds used, we obtained novel derivatives via reaction of the starting material with NBD-chloride. Therefore, we performed a full structural analysis for these compounds, i.e., elemental analysis, IR, UV-Vis, 1H- and 13C-NMR, ESI-MS, X-ray diffraction on single crystal, etc. The hydrophobicity of all the compounds was measured either experimentally using the RP-TLC technique, or via calculation using the fragments method. The other structural characteristics were analyzed, and a correlation between the experimental and computed properties was found. Moreover, the results of the biological evaluation showed that some of the synthesized compounds have antimicrobial and antibiofilm activity.
Caffeic Acid Phenethyl Ester Suppresses Oxidative Stress and Regulates M1/M2 Microglia Polarization via Sirt6/Nrf2 Pathway to Mitigate Cognitive Impairment in Aged Mice following Anesthesia and Surgery
Authors: Wang Yue , Cai Ziwen , Zhan Gaofeng , Li Xing , Li Shan , Wang Xuan , Li Shiyong , Luo Ailin
Published: March 2023

Postoperative cognitive dysfunction (POCD) is a severe neurological complication after anesthesia and surgery. However, there is still a lack of effective clinical pharmacotherapy due to its unclear pathogenesis. Caffeic acid phenethyl ester (CAPE), which is obtained from honeybee propolis and medicinal plants, shows powerful antioxidant, anti-inflammatory, and immunomodulating properties. In this study, we aimed to evaluate whether CAPE mitigated cognitive impairment following anesthesia and surgery and its potential underlying mechanisms in aged mice. Here, isoflurane anesthesia and tibial fracture surgery were used as the POCD model, and H2O2-induced BV2 cells were established as the microglial oxidative stress model. We revealed that CAPE pretreatment suppressed oxidative stress and promoted the switch of microglia from the M1 to the M2 type in the hippocampus, thereby ameliorating cognitive impairment caused by anesthesia and surgery. Further investigation indicated that CAPE pretreatment upregulated hippocampal Sirt6/Nrf2 expression after anesthesia and surgery. Moreover, mechanistic studies in BV2 cells demonstrated that the potent effects of CAPE pretreatment on reducing ROS generation and promoting protective polarization were attenuated by a specific Sirt6 inhibitor, OSS_128167. In summary, our findings opened a promising avenue for POCD prevention through CAPE pretreatment that enhanced the Sirt6/Nrf2 pathway to suppress oxidative stress as well as favor microglia protective polarization.
Correlation Analysis between Dietary Intake of Tyrosols and Their Food Sources and Urinary Excretion of Tyrosol and Hydroxytyrosol in a European Population
Authors: Almanza-Aguilera Enrique , Davila-Cordova Estefanía , Guiñón-Fort Daniel , Farràs Marta , Masala Giovanna , Santucci de Magistris Maria , Baldassari Ivan , Tumino Rosario , Padroni Lisa , Katzke Verena A , Schulze Matthias B. , Scalbert Augustin , Zamora-Ros Raul
Published: March 2023

This study analyzed the correlations between the acute and habitual intake of dietary tyrosols, their main food sources, and 24 h urine excretions of tyrosol (Tyr) and hydroxytyrosol (OHTyr) in participants from the European Prospective Investigation into Cancer and Nutrition study (EPIC). Participants (n = 419) were healthy men and women aged from 34 to 73 years from 8 EPIC centers belonging to France, Italy, and Germany. Acute and habitual dietary data were collected using a standardized 24 h dietary recall software and validated country-specific dietary questionnaires, respectively. The intake of 13 dietary tyrosols was estimated using the Phenol-Explorer database. Excretions of Tyr and OHTyr in a single 24 h urine sample were analyzed using tandem mass spectrometry. Urinary excretions of Tyr, OHTyr, and their sum (Tyr + OHTyr) correlated more strongly with their corresponding acute (rhopartial~0.63) rather than habitual intakes (rhopartial~0.47). In addition, individual and combined urinary excretions of Tyr and OHTyr were weakly to moderately correlated with the acute and habitual intake of other individual tyrosol precursors (rhopartial = 0.10–0.44) and especially with major food sources, such as wine (rhopartial = 0.41–0.58), olive oil (rhopartial = 0.25–0.44), and beer (rhopartial = 0.14–0.23). Urinary Tyr + OHTyr excretions were similarly correlated with the acute intake of total tyrosols but differently correlated with food sources among countries. Based on these results, we conclude that 24 h urinary excretions of Tyr + OHTyr could be proposed as biomarkers of total tyrosol intake, preferably for acute intakes.
Exploring the Nutritional Composition and Bioactive Compounds in Different Cocoa Powders
Authors: Razola-Díaz María del Carmen , Aznar-Ramos María José , Verardo Vito , Melgar-Locatelli Sonia , Castilla-Ortega Estela , Rodríguez-Pérez Celia
Published: March 2023

Cocoa, the main derivative of the seeds of Theobroma cacao L., has been recognized to have several effects on human health including antioxidant and neuro- and cardio-protective effects, among others. These effects have been attributed mainly to its bioactive compounds. In this context, the aim of this work is to evaluate the nutritional composition, bioactive compounds (i.e., phenolic compounds, procyanidins and methylxanthines) and the antioxidant activity of seven different cocoas (alkalized and non-alkalized) from different origins (Peru, Venezuela, Ivory Coast, Dominican Republic, and West Africa). It represents the first stage of a larger project aiming to find high polyphenol cocoa-based nutritional strategies and related biomarkers that may potentiate brain plasticity and cognitive function. Cocoa powders were extracted by ultrasound-assisted technology, and the total phenolic content (TPC) was measured by Folin–Ciocalteu. Methylxanthines (caffeine and theobromine) and procyanidin contents were determined by HPLC-FLD-DAD, and the antioxidant activity was assessed through DPPH, ABTS and FRAP assays. Non-alkalized cocoas showed higher phenolic and procyanidin contents and higher antioxidant activity compared to the alkalized ones. A strongly significant (p < 0.05) positive correlation between the antioxidant activity and the TPC, especially with the total procyanidin content, but not with methylxanthines was found. In conclusion, the non-alkalized cocoas, especially the one from Peru, were the best candidates in terms of bioactive compounds. The cocoa from Peru had a TPC of 57.4 ± 14.4 mg of gallic acid equivalent/g d.w., 28,575.06 ± 62.37 µg of catechin equivalents/g d.w., and 39.15 ± 2.12 mg/g of methylxanthines. Further studies should be undertaken to evaluate its effect on brain plasticity and cognitive function.
Neuroimaging in Primary Coenzyme-Q10-Deficiency Disorders
Authors: Münch Juliane , Prasuhn Jannik , Laugwitz Lucia , Fung Cheuk-Wing , Chung Brian H.-Y. , Bellusci Marcello , Mayatepek Ertan , Klee Dirk , Distelmaier Felix
Published: March 2023

Coenzyme Q10 (CoQ10) is an endogenously synthesized lipid molecule. It is best known for its role as a cofactor within the mitochondrial respiratory chain where it functions in electron transfer and ATP synthesis. However, there are many other cellular pathways that also depend on the CoQ10 supply (redox homeostasis, ferroptosis and sulfide oxidation). The CoQ10 biosynthesis pathway consists of several enzymes, which are encoded by the nuclear DNA. The majority of these enzymes are responsible for modifications of the CoQ-head group (benzoquinone ring). Only three enzymes (PDSS1, PDSS2 and COQ2) are required for assembly and attachment of the polyisoprenoid side chain. The head-modifying enzymes may assemble into resolvable domains, representing COQ complexes. During the last two decades, numerous inborn errors in CoQ10 biosynthesis enzymes have been identified. Thus far, 11 disease genes are known (PDSS1, PDSS2, COQ2, COQ4, COQ5, COQ6, COQ7, COQ8A, COQ8B, COQ9 and HPDL). Disease onset is highly variable and ranges from the neonatal period to late adulthood. CoQ10 deficiency exerts detrimental effects on the nervous system. Potential consequences are neuronal death, neuroinflammation and cerebral gliosis. Clinical features include encephalopathy, regression, movement disorders, epilepsy and intellectual disability. Brain magnetic resonance imaging (MRI) is the most important tool for diagnostic evaluation of neurological damage in individuals with CoQ10 deficiency. However, due to the rarity of the different gene defects, information on disease manifestations within the central nervous system is scarce. This review aims to provide an overview of brain MRI patterns observed in primary CoQ10 biosynthesis disorders and to highlight disease-specific findings.
Metformin Impacts Human Syncytiotrophoblast Mitochondrial Function from Pregnancies Complicated by Obesity and Gestational Diabetes Mellitus in a Sexually Dimorphic Manner
Authors: Hebert Jessica F. , Myatt Leslie
Published: March 2023

Maternal obesity and gestational diabetes mellitus (GDM) are associated with placental dysfunction, small for gestational age (SGA) offspring, and programming of adult-onset disease. We examine how metformin, commonly used to treat type A2 GDM, affects placental metabolism as well as mitochondrial content and function. Syncytiotrophoblasts (STBs) were prepared from placentas of male and female fetuses collected at term cesarean section from lean (pre-pregnancy BMI < 25), obese (BMI > 30), and obese A2GDM women. Metformin treatment (0.001–10 mM) of STB caused no change in non-mitochondrial respiration but significant concentration-dependent (1 and 10 mM) decreases in basal, maximal, and ATP-linked respiration and spare capacity. Respiration linked to proton leak was significantly increased in STB of male A2GDM placentas at low metformin concentrations. Metformin concentrations ≥1 mM increased glycolysis in STB from placentas from lean women, but only improved glycolytic capacity in female STB. Whereas metformin had little effect on superoxide generation from male STB of any group, it gave a concentration-dependent decrease in superoxide generation from female STB of lean and obese women. Fewer mitochondria were observed in STB from obese women and male STB from lean women with increasing metformin concentration. Metformin affects STB mitochondrial function in a sexually dimorphic manner but at concentrations above those reported in maternal circulation (approximately 0.01 mM) in women treated with metformin for GDM.