bluuebunny/crossref_metadata_2025 huggingface dataset:
Operando X‐ray Diffraction Characterization for Understanding the Intrinsic Electrochemical Mechanism in Rechargeable Battery Materials
Authors: Wei Xiujuan , Wang Xuanpeng , An Qinyou , Han Chunhua , Mai Liqiang
Published: April 2017

Studying and understanding the physical and chemical changes in electrode materials during cycling is important for achieving high‐performance rechargeable batteries. Operando X‐ray diffraction (XRD), as an efficient analytic method, has been developed to probe the phase transitions and crystal‐structure changes in electrodes. Recent progress in studying the electrochemical reaction process of electrodes during cycling by the operando XRD analysis method is summarized. The applications of operando XRD toward both cathode and anode materials of rechargeable batteries are summarized, including polyanionic compounds, layered oxides, and insertion‐, conversion‐ and alloying‐type anodes. The correlation between the structure changes and electrochemical performance of electrode materials is systematically analyzed. Finally, the challenges and perspectives on the development of the operando XRD characterization method in electrode materials are introduced and discussed.
Modeling and efficient verification of wireless ad hoc networks
Authors: Yousefi Behnaz , Ghassemi Fatemeh , Khosravi Ramtin
Published: April 2017

Wireless ad hoc networks, in particular mobile ad hoc networks (MANETs), are growing very fast as they make communication easier and more available. However, their protocols tend to be difficult to design due to topology dependent behavior of wireless communication, and their distributed and adaptive operations to topology dynamism. Therefore, it is desirable to have them modeled and verified using formal methods. In this paper, we present an actor-based modeling language with the aim to model MANETs. We address main challenges of modeling wireless ad hoc networks such as local broadcast, underlying topology, and its changes, and discuss how they can be efficiently modeled at the semantic level to make their verification amenable. The new framework abstracts the data link layer services by providing asynchronous (local) broadcast and unicast communication, while message delivery is in order and is guaranteed for connected receivers. We illustrate the applicability of our framework through two routing protocols, namely flooding and AODVv2-11, and show how efficiently their state spaces can be reduced by the proposed techniques. Furthermore, we demonstrate a loop formation scenario in AODV, found by our analysis tool.
Ground states of pseudo-relativistic boson stars under the critical stellar mass
Authors: Guo Yujin , Zeng Xiaoyu
Published: April 2017

We consider ground states of pseudo-relativistic boson stars with a self-interacting potential

K(x)

in

\mathbb{R}^{3}

, which can be described by minimizers of the pseudo-relativistic Hartree energy functional. Under some assumptions on

K(x)

, minimizers exist if the stellar mass
N
satisfies

0 < N < N^{⁎}

, and there is no minimizer if

N > N^{⁎}

, where

N^{⁎}

is called the critical stellar mass. In contrast to the case of the Coulomb-type potential where

K(x) \equiv 1

, we prove that the existence of minimizers may occur at

N = N^{⁎}

, depending on the local profile of

K(x)

near the origin. When there is no minimizer at

N = N^{⁎}

, we also present a detailed analysis of the behavior of minimizers as
N
approaches

N^{⁎}

from below, for which the stellar mass concentrates at a unique point.
FILAC – Fistula – Tract Laser Closure: a sphincter-preserving procedure for the treatment of complex anal fistulas
Authors: Carvalho Alexandre Lopes de , Filho Eduardo Fonseca Alves , Alcantara Rogerio Souza Medrado de , Barreto Marcelo da Silva
Published: April 2017

Several surgical options are described for the treatment of anorectal fistulas, particularly in complex cases where recurrence rates and the possibility of postoperative incontinence are still high. The aim of this study is to describe the use of FILAC technique (Fistula – Tract Laser Closure) minimally invasive and preservation sphincter technique. FILAC has been described in the literature as an option in the management of anorectal fistula.
Semantics for Counting and Measuring
Authors: Rothstein Susan
Published: April 2017

The use of numerals in counting differs quite dramatically across languages. Some languages grammaticalise a contrast between count nouns (three cats; three books) vs 'non-count' or mass nouns (milk, mud), marking this distinction in different ways. Others use a system of numeral classifiers, while yet others use a combination of both. This book draws attention to the contrast between counting and measuring, and shows that it is central to our understanding of how we use numerical expressions, classifiers and count nouns in different languages. It reviews some of the more recent major linguistic results in the semantics of numericals, counting and measuring and theories of the mass/count distinction, and presents the author's new research on the topic. The book draws heavily on crosslinguistic research, and presents in-depth case studies of the mass/count distinction and counting and measuring in a number of typologically unrelated languages. It also includes chapters on classifiers, constructions and on adjectival uses of measure phrases.
In gratitude for grace: praise, worship and the sanctified life
Authors: Greggs Tom
Published: April 2017

This article seeks to offer a theological account of gratitude from a Protestant perspective by arguing that the Christian life is one marked by a covenant of grace and gratitude in which the creature's response of gratitude to divine grace is a participating in that grace, and, as such, is a full recognition of the ways of grace which flow from the divine life to creation. The first section of this article examines this theme in relation to creation from nothing. The second establishes God's electing will as the foundation of God's willing to be for another in creation. The third section examines justification by grace as the soteriological form of God's gracious turning towards the creature in divine mercy. The fourth section sketches the forms of gratitude that one might find in the Christian life as these are brought together in the sacraments of baptism and the eucharist.
Afro-Futurism or Lament? Staging Africa(s) in Dance Today and in the 1920s
Authors: Haitzinger Nicole
Published: April 2017

This article analyzes two contemporary pieces, Faustin Linyekula's La Création du Monde 1923–2012 and Vera Mantero's A mysterious thing said e. e. Cummings, which respond to dance productions presented in Europe in the early decades of the twentieth century by criticizing their “negrophilic” attitude. The article juxtaposes the analysis of these two contemporary pieces with rereadings of the historical choreographies/events of the 1920s to which they refer, namely, Les Ballets Suédois's La Création du Monde (1923) and Josephine Baker's performances. Theoretically revisiting historical works that developed within such a “negrophilic” framework alongside contemporary pieces relating to them can be taken as attacking this very framework, trying to “undo” the Eurocentrism inherent in its cannibalistic processes. Such a perspective may allow for the acknowledgement of plural, multiple views of Africanistic presences in an otherwise “negrophilic” context.
A Short Article on a Lively Subject: Geltruda Rossi, Sarah Siddons, and Shakespeare's Lady Macbeth à la Fuseli
Authors: Burden Michael
Published: April 2017

In the 1784–85 London season, there was a conjunction between Shakespeare's Macbeth and three of the most thrilling artists of the eighteenth century: the dancer Geltruda Rossi, the actress Sarah Siddons, and the artist Henry Fuseli. They were thrown into figurative proximity when a commentator on Rossi's performance wrote in the Morning Herald and Daily Advertiser: “Madame Rossi, in Lady Macbeth, impresses one more with the recollection of Fusili’s [sic] painting, than of Mrs Siddons’s representation—indeed comparison would be doing an injustice to our critical and admired English performer.” While we know much about the art of Siddons and Fuseli, we know little about Rossi's performances, which makes the Morning Herald’s parallel worth exploring as an example of eighteenth-century London ballet d'action.
Conflict management in western Europe: the case of the Portuguese merchants in England, Flanders and Normandy, 1250–1500
Authors: MIRANDA FLÁVIO
Published: April 2017

Recent historiography argues that the legal autonomy of municipal governments created the necessary conditions for successful commercial transactions and economic growth in certain parts of Europe in the later Middle Ages, and that these features attracted foreign merchants. This article uses empirical data from England, Flanders and Normandy to test the following questions: were there significant differences in rules, laws and institutions between one place and another in late medieval western Europe? Were the Portuguese merchants drawn to markets that hypothetically had more effective institutions? The findings demonstrate that legal institutions and conflict management were very similar across western Europe, and that there is no evidence that the Portuguese opted for trading in a certain market because of its effective institutions. Moreover, the article claims that the merchants seemed to prioritise protection and privilege while trading abroad, and it highlights the role of commercial diplomacy in conflict management.
Association of cognitive function and liability to addiction with childhood herpesvirus infections: A prospective cohort study
Authors: Vanyukov Michael M. , Nimgaonkar Vishwajit L. , Kirisci Levent , Kirillova Galina P. , Reynolds Maureen D. , Prasad Konasale , Tarter Ralph E. , Yolken Robert H.
Published: April 2017

Liability to substance use disorder (SUD) is largely nonspecific to particular drugs and is related to behavior dysregulation, including reduced cognitive control. Recent data suggest that cognitive mechanisms may be influenced by exposure to neurotropic infections, such as human herpesviruses. In this study, serological evidence of exposure to human herpesvirus Herpes simplex virus Type 1 (HSV-1), cytomegalovirus (CMV), and Epstein–Barr virus (EBV) as well as Toxoplasma gondii was determined in childhood (age ~11 years) in 395 sons and 174 daughters of fathers with or without SUD. Its relationships with a cognitive characteristic (IQ) in childhood and with risk for SUD in adulthood were examined using correlation, regression, survival, and path analyses. Exposure to HSV-1, EBV, and T. gondii in males and females, and CMV in males, was associated with lower IQ. Independent of that relationship, EBV in females and possibly in males, and CMV and possibly HSV-1 in females were associated with elevated risk for SUD. Therefore, childhood neurotropic infections may influence cognitive development and risk for behavior disorders such as SUD. The results may point to new avenues for alleviating cognitive impairment and SUD risk.
Environmental standards, trade and innovation: evidence from a natural experiment
Authors: Chakraborty Pavel
Published: April 2017

Exploiting a natural experiment involving the imposition of a technical regulation by Germany on Indian leather and textile industries in 1994, a firm-level data set is used to study the trade, adaptation and discontinuity effects and how they vary by firm size. It is found that: (a) regulation significantly increases the export revenues of a firm through use of new technology and high-quality imported raw materials – indicating a possible signalling effect; (b) this gain is concentrated only on the upper half of the firm size distribution, i.e., in the 3rd and 4th quartiles; (c) use of imported raw materials significantly explains low exit probabilities of a firm; and (d) there is evidence of a sorting effect – regulation significantly affecting the operation of small firms.
THE CONVERGENCE AND WEAK LAWS OF LARGE NUMBERS FOR -MIXING RANDOM VARIABLES
Authors: MENG YAN-JIAO
Published: April 2017

The $L_{r}$ convergence and a class of weak laws of large numbers are obtained for sequences of $\widetilde{\unicode[STIX]{x1D70C}}$-mixing random variables under the uniform Cesàro-type condition. This is weaker than the $p$th-order Cesàro uniform integrability.
Thermogenic adipocytes promote HDL turnover and reverse cholesterol transport
Authors: Bartelt Alexander , John Clara , Schaltenberg Nicola , Berbée Jimmy F. P. , Worthmann Anna , Cherradi M. Lisa , Schlein Christian , Piepenburg Julia , Boon Mariëtte R. , Rinninger Franz , Heine Markus , Toedter Klaus , Niemeier Andreas , Nilsson Stefan K. , Fischer Markus , Wijers Sander L. , van Marken Lichtenbelt Wouter , Scheja Ludger , Rensen Patrick C. N. , Heeren Joerg
Published: April 2017

Brown and beige adipocytes combust nutrients for thermogenesis and through their metabolic activity decrease pro-atherogenic remnant lipoproteins in hyperlipidemic mice. However, whether the activation of thermogenic adipocytes affects the metabolism and anti-atherogenic properties of high-density lipoproteins (HDL) is unknown. Here, we report a reduction in atherosclerosis in response to pharmacological stimulation of thermogenesis linked to increased HDL levels in APOE*3-Leiden.CETP mice. Both cold-induced and pharmacological thermogenic activation enhances HDL remodelling, which is associated with specific lipidomic changes in mouse and human HDL. Furthermore, thermogenic stimulation promotes HDL-cholesterol clearance and increases macrophage-to-faeces reverse cholesterol transport in mice. Mechanistically, we show that intravascular lipolysis by adipocyte lipoprotein lipase and hepatic uptake of HDL by scavenger receptor B-I are the driving forces of HDL-cholesterol disposal in liver. Our findings corroborate the notion that high metabolic activity of thermogenic adipocytes confers atheroprotective properties via increased systemic cholesterol flux through the HDL compartment.
Impact of hormone receptor status and distant recurrence-free interval on survival benefits from trastuzumab in HER2-positive metastatic breast cancer
Authors: Yang Hai-Yuan , Ma Ding , Liu Yi-Rong , Hu Xin , Zhang Jian , Wang Zhong-Hua , Di Gen-Hong , Hu Xi-Chun , Shao Zhi-Ming
Published: April 2017

We sought to investigate the impact of hormone receptor (HR) status and distant recurrence-free interval (DRFI) on the degree of overall survival (OS) benefit from palliative trastuzumab-containing treatment in HER2-positive metastatic breast cancer (MBC). Here, we retrospectively identified 588 eligible HER2-positive patients with postoperative distant recurrence. DRFI of HR+HER2+ MBC patients (median: 30.7 months, IQR: 18.5–45.9, P < 0.001) was significant longer compared with HR−HER2+ patients. Patients were categorized into four subgroups based on HR status and palliative trastuzumab (trast+) received. The most superior outcome was observed in the HR+HER2+trast+ subgroup, with a median OS of 48.3 months. Moreover, DRFI > 24 months is an independent favourable prognostic factor for both HR−HER2+ patients (Hazard Ratio (HzR) = 0.55, 95% CI: 0.39–0.76, P < 0.001) and HR+HER2+ patients (HzR = 0.45, 95% CI: 0.32–0.64, P < 0.001). Upon further analysis of the interaction between trastuzumab and DRFI, the degree of trastuzumab benefits in HR−HER2+ MBC patients remained basically unchanged regardless of DRFI length. Unlikely, the degree in HR+HER2+ MBC patients decreased gradually along with DRFI extending, indicating that trastuzumab failed to translate into an OS benefit for late recurrent (DRFI > 5years) HR+HER2+ MBC patients.
Lsh/HELLS regulates self-renewal/proliferation of neural stem/progenitor cells
Authors: Han Yixing , Ren Jianke , Lee Eunice , Xu Xiaoping , Yu Weishi , Muegge Kathrin
Published: April 2017

Epigenetic mechanisms are known to exert control over gene expression and determine cell fate. Genetic mutations in epigenetic regulators are responsible for several neurologic disorders. Mutations of the chromatin remodeling protein Lsh/HELLS can cause the human Immunodeficiency, Centromere instability and Facial anomalies (ICF) syndrome, which is associated with neurologic deficiencies. We report here a critical role for Lsh in murine neural development. Lsh depleted neural stem/progenitor cells (NSPCs) display reduced growth, increases in apoptosis and impaired ability of self-renewal. RNA-seq analysis demonstrates differential gene expression in Lsh−/− NSPCs and suggests multiple aberrant pathways. Concentrating on specific genomic targets, we show that ablation of Lsh alters epigenetic states at specific enhancer regions of the key cell cycle regulator Cdkn1a and the stem cell regulator Bmp4 in NSPCs and alters their expression. These results suggest that Lsh exerts epigenetic regulation at key regulators of neural stem cell fate ensuring adequate NSPCs self-renewal and maintenance during development.
Dynamics of chromatin accessibility during TGF-β-induced EMT of Ras-transformed mammary gland epithelial cells
Authors: Arase Mayu , Tamura Yusuke , Kawasaki Natsumi , Isogaya Kazunobu , Nakaki Ryo , Mizutani Anna , Tsutsumi Shuichi , Aburatani Hiroyuki , Miyazono Kohei , Koinuma Daizo
Published: April 2017

Epithelial-mesenchymal transition (EMT) is induced by transforming growth factor (TGF)-β and facilitates tumor progression. We here performed global mapping of accessible chromatin in the mouse mammary gland epithelial EpH4 cell line and its Ras-transformed derivative (EpRas) using formaldehyde-assisted isolation of regulatory element (FAIRE)-sequencing. TGF-β and Ras altered chromatin accessibility either cooperatively or independently, and AP1, ETS, and RUNX binding motifs were enriched in the accessible chromatin regions of EpH4 and EpRas cells. Etv4, an ETS family oncogenic transcription factor, was strongly expressed and bound to more than one-third of the accessible chromatin regions in EpRas cells treated with TGF-β. While knockdown of Etv4 and another ETS family member Etv5 showed limited effects on the decrease in the E-cadherin abundance and stress fiber formation by TGF-β, gene ontology analysis showed that genes encoding extracellular proteins were most strongly down-regulated by Etv4 and Etv5 siRNAs. Accordingly, TGF-β-induced expression of Mmp13 and cell invasiveness were suppressed by Etv4 and Etv5 siRNAs, which were accompanied by the reduced chromatin accessibility at an enhancer region of Mmp13 gene. These findings suggest a mechanism of transcriptional regulation during Ras- and TGF-β-induced EMT that involves alterations of accessible chromatin, which are partly regulated by Etv4 and Etv5.
Double-stranded DNA induces a prothrombotic phenotype in the vascular endothelium
Authors: Gaitzsch Erik , Czermak Thomas , Ribeiro Andrea , Heun Yvonn , Bohmer Monica , Merkle Monika , Mannell Hanna , Schulz Christian , Wörnle Markus , Pircher Joachim
Published: April 2017

Double-stranded DNA (dsDNA) constitutes a potent activator of innate immunity, given its ability to bind intracellular pattern recognition receptors during viral infections or sterile tissue damage. While effects of dsDNA in immune cells have been extensively studied, dsDNA signalling and its pathophysiological implications in non-immune cells, such as the vascular endothelium, remain poorly understood. The aim of this study was to characterize prothrombotic effects of dsDNA in vascular endothelial cells. Transfection of cultured human endothelial cells with the synthetic dsDNA poly(dA:dT) induced upregulation of the prothrombotic molecules tissue factor and PAI-1, resulting in accelerated blood clotting in vitro, which was partly dependent on RIG-I signalling. Prothrombotic effects were also observed upon transfection of endothelial cells with hepatitis B virus DNA-containing immunoprecipitates as well human genomic DNA. In addition, dsDNA led to surface expression of von Willebrand factor resulting in increased platelet-endothelium-interactions under flow. Eventually, intrascrotal injection of dsDNA resulted in accelerated thrombus formation upon light/dye-induced endothelial injury in mouse cremaster arterioles and venules in vivo. In conclusion, we show that viral or endogenous dsDNA induces a prothrombotic phenotype in the vascular endothelium. These findings represent a novel link between pathogen- and danger-associated patterns within innate immunity and thrombosis.
Direct Formation of Structural Components Using a Martian Soil Simulant
Authors: Chow Brian J. , Chen Tzehan , Zhong Ying , Qiao Yu
Published: April 2017

Martian habitats are ideally constructed using only locally available soils; extant attempts to process structural materials on Mars, however, generally require additives or calcination. In this work we demonstrate that Martian soil simulant Mars-1a can be directly compressed at ambient into a strong solid without additives, highlighting a possible aspect of complete Martian in-situ resource utilization. Flexural strength of the compact is not only determined by the compaction pressure but also significantly influenced by the lateral boundary condition of processing loading. The compression loading can be applied either quasi-statically or through impact. Nanoparticulate iron oxide (npOx), commonly detected in Martian regolith, is identified as the bonding agent. Gas permeability of compacted samples was measured to be on the order of 10−16 m2, close to that of solid rocks. The compaction procedure is adaptive to additive manufacturing.
Hybrid Energy Storage of Ni(OH)2-coated N-doped Graphene Aerogel//N-doped Graphene Aerogel for the Replacement of NiCd and NiMH Batteries
Authors: Sirisinudomkit Pichamon , Iamprasertkun Pawin , Krittayavathananon Atiweena , Pettong Tanut , Dittanet Peerapan , Sawangphruk Montree
Published: April 2017

Although Nickel–Cadmium (NiCd) and Nickel–metal hydride (NiMH) batteries have been widely used, their drawbacks including toxic Cd and expensive La alloy at the negative electrodes, low energy density (40–60 Wh/kg for NiCd and 140–300 Wh/L for NiMH), low power density (150 W/kg for NiCd and 1000 W/kg for NiMH), and low working potential (1.2 V) limit their applications. In this work, Cd and La alloy were replaced with N-doped reduced graphene oxide aerogel (N-rGOae) providing a hybrid energy storage (HES) having the battery and supercapacitor effects. The HES of Ni(OH)2-coated N-rGOae//N-rGOae provides 1.5 V, a specific energy of 146 Wh/kg, a maximum specific power of 7705 W/kg, and high capacity retention over 84.6% after 5000 cycles. The mass change at the positive electrode during charging/discharging is 8.5 µg cm−2 owing to the insertion/desertion of solvated OH− into the α-Ni(OH)2-coated N-rGOae. At the negative electrode, the mass change of the solvated K+, physically adsorbed/desorbed to the N-rGOae, is 7.5 μg cm−2. In situ X-ray absorption spectroscopy (XAS) shows highly reversible redox reaction of α-Ni(OH)2. The as-fabricated device without using toxic Cd and expensive La alloy has a potential as a candidate of NiCd and NiMH.
Soret and Dufour effects on unsteady mixed convection slip flow of Casson fluid over a nonlinearly stretching sheet with convective boundary condition
Authors: Ullah Imran , Khan Ilyas , Shafie Sharidan
Published: April 2017

Unsteady mixed convection flow of Casson fluid towards a nonlinearly stretching sheet with the slip and convective boundary conditions is analyzed in this work. The effects of Soret Dufour, viscous dissipation and heat generation/absorption are also investigated. After using some suitable transformations, the unsteady nonlinear problem is solved by using Keller-box method. Numerical solutions for wall shear stress and high temperature transfer rate are calculated and compared with previously published work, an excellent arrangement is followed. It is noticed that fluid velocity reduces for both local unsteadiness and Casson parameters. It is likewise noticed that the influence of a Dufour number of dimensionless temperature is more prominent as compared to species concentration. Furthermore, the temperature was found to be increased in the case of nonlinear thermal radiation.
Pentamethylquercetin induces adipose browning and exerts beneficial effects in 3T3-L1 adipocytes and high-fat diet-fed mice
Authors: Han Yi , Wu Jian-Zhao , Shen Ji-zhong , Chen Lei , He Ting , Jin Man-wen , Liu Hui
Published: April 2017

Browning white adipocytes may be a new target in anti-obesity therapy. Pentamethylquercetin (PMQ) has been shown to have anti-obesity effects in monosodium glutamate-induced obese mice. Here, we aimed to study the anti-obesity effects of PMQ in vitro and in vivo and to determine if adipose browning is involved in the mechanism underlying the anti-obesity effects of PMQ. We evaluated the effects of PMQ on cell proliferation, cell differentiation, glucose consumption, cellular lipid metabolism, and related brown gene expression in 3T3-L1 adipocytes. We also investigated the effects of PMQ in a mouse model of high-fat diet (HFD)-induced obesity. Our results demonstrated that PMQ increased the consumption of glucose, inhibited the accumulation of cellular triglycerides (TGs), and induced the expression of brown adipocyte-specific genes, such as uncoupling protein 1 (UCP-1), during the early stage of differentiation in 3T3-L1 adipocytes. In HFD mice, PMQ treatment reduced waist circumference, LEE index, white adipose tissue (WAT) weight and white adipocyte size and increased brown adipose tissue (BAT) weight. Moreover, PMQ treatment induced mitochondrial biogenesis and upregulated UCP-1 expression in WAT. These findings suggest that PMQ may induce browning of adipose tissue, a phenomenon that is at least partly related to its anti-obesity effects.
Non-volatile logic gates based on planar Hall effect in magnetic films with two in-plane easy axes
Authors: Lee Sangyeop , Bac Seul-Ki , Choi Seonghoon , Lee Hakjoon , Yoo Taehee , Lee Sanghoon , Liu Xinyu , Dobrowolska M. , Furdyna Jacek K.
Published: April 2017

We discuss the use of planar Hall effect (PHE) in a ferromagnetic GaMnAs film with two in-plane easy axes as a means for achieving novel logic functionalities. We show that the switching of magnetization between the easy axes in a GaMnAs film depends strongly on the magnitude of the current flowing through the film due to thermal effects that modify its magnetic anisotropy. Planar Hall resistance in a GaMnAs film with two in-plane easy axes shows well-defined maxima and minima that can serve as two binary logic states. By choosing appropriate magnitudes of the input current for the GaMnAs Hall device, magnetic logic functions can then be achieved. Specifically, non-volatile logic functionalities such as AND, OR, NAND, and NOR gates can be obtained in such a device by selecting appropriate initial conditions. These results, involving a simple PHE device, hold promise for realizing programmable logic elements in magnetic electronics.
Pressure-induced structural change in liquid GaIn eutectic alloy
Authors: Yu Q. , Ahmad A. S. , Ståhl K. , Wang X. D. , Su Y. , Glazyrin K. , Liermann H. P. , Franz H. , Cao Q. P. , Zhang D. X. , Jiang J. Z.
Published: April 2017

Synchrotron x-ray diffraction reveals a pressure induced crystallization at about 3.4 GPa and a polymorphic transition near 10.3 GPa when compressed a liquid GaIn eutectic alloy up to ~13 GPa at room temperature in a diamond anvil cell. Upon decompression, the high pressure crystalline phase remains almost unchanged until it transforms to the liquid state at around 2.3 GPa. The ab initio molecular dynamics calculations can reproduce the low pressure crystallization and give some hints on the understanding of the transition between the liquid and the crystalline phase on the atomic level. The calculated pair correlation function g(r) shows a non-uniform contraction reflected by the different compressibility between the short (1st shell) and the intermediate (2nd to 4th shells). It is concluded that the pressure-induced liquid-crystalline phase transformation likely arises from the changes in local atomic packing of the nearest neighbors as well as electronic structures at the transition pressure.
The biomarker and causal roles of homoarginine in the development of cardiometabolic diseases: an observational and Mendelian randomization analysis
Authors: Seppälä Ilkka , Oksala Niku , Jula Antti , Kangas Antti J. , Soininen Pasi , Hutri-Kähönen Nina , März Winfried , Meinitzer Andreas , Juonala Markus , Kähönen Mika , Raitakari Olli T. , Lehtimäki Terho
Published: April 2017

High L-homoarginine (hArg) levels are directly associated with several risk factors for cardiometabolic diseases whereas low levels predict increased mortality in prospective studies. The biomarker role of hArg in young adults remains unknown. To study the predictive value of hArg in the development of cardiometabolic risk factors and diseases, we utilized data on high-pressure liquid chromatography-measured hArg, cardiovascular risk factors, ultrasound markers of preclinical atherosclerosis and type 2 diabetes from the population-based Young Finns Study involving 2,106 young adults (54.6% females, aged 24–39). We used a Mendelian randomization approach involving tens to hundreds of thousands of individuals to test causal associations. In our 10-year follow-up analysis, hArg served as an independent predictor for future hyperglycaemia (OR 1.31, 95% CI 1.06–1.63) and abdominal obesity (OR 1.60, 95% 1.14–2.30) in men and type 2 diabetes in women (OR 1.55, 95% CI 1.02–2.41). The MR analysis revealed no evidence of causal associations between serum hArg and any of the studied cardiometabolic outcomes. In conclusion, lifetime exposure to higher levels of circulating hArg does not seem to alter cardiometabolic disease risk. Whether hArg could be used as a biomarker for identification of individuals at risk developing cardiometabolic abnormalities merits further investigation.
Design principles governing chemomechanical coupling of kinesin
Authors: Sumi Tomonari
Published: April 2017

A systematic chemomechanical network model for the molecular motor kinesin is presented in this report. The network model is based on the nucleotide-dependent binding affinity of the heads to an microtubule (MT) and the asymmetries and similarities between the chemical transitions caused by the intramolecular strain between the front and rear heads. The network model allows for multiple chemomechanical cycles and takes into account all possible mechanical transitions between states in which one head is strongly bound and the other head is weakly bound to an MT. The results obtained from the model show the ATP-concentration dependence of the dominant forward stepping cycle and support a gated rear head mechanism in which the forward step is controlled by ATP hydrolysis and the resulting ADP-bound state of the rear head when the ATP level is saturated. When the ATP level is saturated, the energy from ATP hydrolysis is used to concentrate the chemical transition flux to a force-generating state that can produce the power stroke. In contrast, when the ATP level is low, the hydrolysis energy is consumed to avoid states in which the leading head is weakly bound to an MT and to inhibit frequent backward steps upon loading.
Designed synthesis of a “One for Two” hydrophilic magnetic amino-functionalized metal-organic framework for highly efficient enrichment of glycopeptides and phosphopeptides
Authors: Xie Yiqin , Deng Chunhui
Published: April 2017

Highly efficient enrichment of glycopeptides or phosphopeptides from complex biological samples is indispensable for high-throughput mass spectrometry analysis. In this study, for the first time, a “one for two” hydrophilic magnetic amino-functionalized metal-organic framework (MOF) was designed and synthesized for selective enrichment of both glycopeptides and phosphopeptides. A well-known solvo-thermal reaction was adopted to prepare a magnetic core Fe3O4, followed by self- polymerization of dopamine, creating a polydopamine (PDA) onto Fe3O4. Thanks to the hydroxyl and amino group of PDA, Zr3+ was easily adhered to the surface, inducing the following one-pot MOF reaction with amino ligand. After characterization of the as-prepared MOFs (denoted as Fe3O4@PDA@UiO-66-NH2), its ultrahigh surface area, excellent hydrophilicity and strong magnetic responsiveness were highly confirmed. Based on hydrophilic interaction, it was applied to glycopeptide enrichment, while based on strong binding between Zr and phosphopeptides, it was applied to phosphopeptide enrichment, both exhibiting excellent performance in standard proteins and human serum with high sensitivity and selectivity. These results showed the as-prepared MOFs had great potential in proteomics research.
The MAPKKK and MAPKK gene families in banana: identification, phylogeny and expression during development, ripening and abiotic stress
Authors: Wang Lianzhe , Hu Wei , Tie Weiwei , Ding Zehong , Ding Xupo , Liu Yang , Yan Yan , Wu Chunlai , Peng Ming , Xu Biyu , Jin Zhiqiang
Published: April 2017

The mitogen-activated protein kinase (MAPK) cascade, which is a major signal transduction pathway widely distributed in eukaryotes, has an important function in plant development and stress responses. However, less information is known regarding the MAPKKK and MAPKK gene families in the important fruit crop banana. In this study, 10 MAPKK and 77 MAPKKK genes were identified in the banana genome, and were classified into 4 and 3 subfamilies respectively based on phylogenetic analysis. Majority of MAPKKK and MAPKK genes in the same subfamily shared similar gene structures and conserved motifs. The comprehensive transcriptome analysis indicated that MAPKKK-MAPKK genes is involved in tissue development, fruit development and ripening, and response to abiotic stress of drought, cold and salt in two banana genotypes. Interaction networks and co-expression assays demonstrated that MAPK signaling cascade mediated network participates in multiple stress signaling, which was strongly activated in Fen Jiao (FJ). The findings of this study advance understanding of the intricately transcriptional control of MAPKKK-MAPKK genes and provide robust candidate genes for further genetic improvement of banana.
Surgical treatment in Paget’s disease with invasive ductal carcinoma: an observational study based on SEER
Authors: Wu Qi , Ding Xiaojun , Li Juanjuan , Sun Si , Zhu Shan , Wu Juan , Liu Qian , Yao Feng , Sun Shengrong
Published: April 2017

The aim is to analyse the clinical presentation, treatment and outcomes in patients with Paget’s disease with invasive ductal carcinoma (PD-IDC), with special emphasis on the role of surgical treatment. Using data obtained by the Surveillance, Epidemiology, and End Results (SEER) program from 2010–2013, we investigated the differences in characteristics, overall survival (OS), and breast cancer-specific mortality (BCSM) between patients with PD-IDC and those with invasive ductal carcinoma (IDC). Compared with IDC group, patients with PD-IDC had a better prognosis and lower mortality in adjusted analyses. In the multivariate analysis of cases with PD-IDC, history of ALND was significantly associated with OS while Her2 status were associated with BCSM. Further, subgroup analysis demonstrated no difference between surgical treatment subgroups for either OS or BCSM. The results demonstrated that PD-IDC appears to alter the association between prognosis and Her2 status. Meanwhile, breast-conserving surgery with radiotherapy may be a feasible treatment alternative and sentinel lymph node biopsy should be considered as an appropriate treatment for patients with PD-IDC.
Clustering by fast search and merge of local density peaks for gene expression microarray data
Authors: Mehmood Rashid , El-Ashram Saeed , Bie Rongfang , Dawood Hussain , Kos Anton
Published: April 2017

Clustering is an unsupervised approach to classify elements based on their similarity, and it is used to find the intrinsic patterns of data. There are enormous applications of clustering in bioinformatics, pattern recognition, and astronomy. This paper presents a clustering approach based on the idea that density wise single or multiple connected regions make a cluster, in which density maxima point represents the center of the corresponding density region. More precisely, our approach firstly finds the local density regions and subsequently merges the density connected regions to form the meaningful clusters. This idea empowers the clustering procedure, in which outliers are automatically detected, higher dense regions are intuitively determined and merged to form clusters of arbitrary shape, and clusters are identified regardless the dimensionality of space in which they are embedded. Extensive experiments are performed on several complex data sets to analyze and compare our approach with the state-of-the-art clustering methods. In addition, we benchmarked the algorithm on gene expression microarray data sets for cancer subtyping; to distinguish normal tissues from tumor; and to classify multiple tissue data sets.
The relationship between climate change and the endangered rainforest shrub Triunia robusta (Proteaceae) endemic to southeast Queensland, Australia
Authors: Shimizu-Kimura Yoko , Accad Arnon , Shapcott Alison
Published: April 2017

Threatened species in rainforests may be vulnerable to climate change, because of their potentially narrow thermal tolerances, small population sizes and restricted distributions. This study modelled climate induced changes on the habitat distribution of the endangered rainforest plant Triunia robusta, endemic to southeast Queensland, Australia. Species distribution models were developed for eastern Australia at 250 m grids and southeast Queensland at 25 m grids using ground-truthed presence records and environmental predictor data. The species’ habitat distribution under the current climate was modelled, and the future potential habitat distributions were projected for the epochs 2030, 2050 and 2070. The eastern Australia model identified several spatially disjunct, broad habitat areas of coastal eastern Australia consistent with the current distribution of rainforests, and projected a southward and upslope contraction driven mainly by average temperatures exceeding current range limits. The southeast Queensland models suggest a dramatic upslope contraction toward locations where the majority of known populations are found. Populations located in the Sunshine Coast hinterland, consistent with past rainforest refugia, are likely to persist long-term. Upgrading the level of protection for less formal nature reserves containing viable populations is a high priority to better protect refugial T. robusta populations with respect to climate change.
Effective in vivo therapeutic IgG antibody against VP3 of enterovirus 71 with receptor-competing activity
Authors: Jia Qiang , Ng Qingyong , Chin Wenjie , Meng Tao , Chow Vincent Tak Kwong , Wang Cheng-I , Kwang Jimmy , He Fang
Published: April 2017

Passive immunization is an effective option for treatment against hand, foot and mouth disease caused by EV71, especially with cross-neutralizing IgG monoclonal antibodies. In this study, an EV71-specific IgG2a antibody designated 5H7 was identified and characterized. 5H7 efficiently neutralizes the major EV71 genogroups (A, B4, C2, C4). The conformational epitope of 5H7 was mapped to the highly conserved amino acid position 74 on VP3 capsid protein using escape mutants. Neutralization with 5H7 is mediated by the inhibition of viral attachment, as revealed by virus-binding and post-attachment assays. In a competitive pull-down assay with SCARB2, 5H7 blocks the receptor-binding site on EV71 for virus neutralization. Passive immunization of chimeric 5H7 protected 100% of two-week-old AG129 mice from lethal challenge with an EV71 B4 strain for both prophylactic and therapeutic treatments. In contrast, 10D3, a previously reported neutralizing antibody that takes effect after virus attachment, could only confer prophylactic protection. These results indicate that efficient interruption of viral attachment is critical for effective therapeutic activity with 5H7. This report documents a novel universal neutralizing IgG antibody for EV71 therapeutics and reveals the underlying mechanism.
Dissecting the genetic architecture of Fusarium verticillioides seed rot resistance in maize by combining QTL mapping and genome-wide association analysis
Authors: Ju Ming , Zhou Zijian , Mu Cong , Zhang Xuecai , Gao Jingyang , Liang Yakun , Chen Jiafa , Wu Yabin , Li Xiaopeng , Wang Shiwei , Wen Jingjing , Yang Luming , Wu Jianyu
Published: April 2017

Fusarium verticillioides can be transmitted via seeds and cause systemic infection in maize (Zea mays L.); its mycotoxin has harmful effects on animal and human health. We combined QTL mapping in recombinant inbred line (RIL) populations with a genome-wide association study (GWAS) of 217 diverse maize lines using 224,152 single nucleotide polymorphisms (SNPs) under controlled conditions to determine the genetic architecture of F. verticillioides seed rot (FSR) resistance. Our study identified 8 quantitative trait loci (QTLs) and 43 genes associated with 57 SNPs that were correlated with FSR resistance through linkage mapping and GWAS, respectively. Among these, there were three candidate genes, namely GRMZM2G0081223, AC213654.3_FG004, and GRMZM2G099255, which were detected in both linkage mapping and GWAS. Furthermore, the near-isogenic lines (NILs) containing GRMZM2G0081223, which also had a susceptible parent background, were found to have a significantly improved level of resistance. In addition, the expression profile of the three candidate genes revealed that they all respond to the infection following inoculation with F. verticillioides. These genetic analyses indicate that FSR resistance is controlled by loci with minor effect, and the polymerization breeding of lines with beneficial alleles and candidate genes could improve FSR resistance in maize.
Monitoring seasonal influenza epidemics by using internet search data with an ensemble penalized regression model
Authors: Guo Pi , Zhang Jianjun , Wang Li , Yang Shaoyi , Luo Ganfeng , Deng Changyu , Wen Ye , Zhang Qingying
Published: April 2017

Seasonal influenza epidemics cause serious public health problems in China. Search queries-based surveillance was recently proposed to complement traditional monitoring approaches of influenza epidemics. However, developing robust techniques of search query selection and enhancing predictability for influenza epidemics remains a challenge. This study aimed to develop a novel ensemble framework to improve penalized regression models for detecting influenza epidemics by using Baidu search engine query data from China. The ensemble framework applied a combination of bootstrap aggregating (bagging) and rank aggregation method to optimize penalized regression models. Different algorithms including lasso, ridge, elastic net and the algorithms in the proposed ensemble framework were compared by using Baidu search engine queries. Most of the selected search terms captured the peaks and troughs of the time series curves of influenza cases. The predictability of the conventional penalized regression models were improved by the proposed ensemble framework. The elastic net regression model outperformed the compared models, with the minimum prediction errors. We established a Baidu search engine queries-based surveillance model for monitoring influenza epidemics, and the proposed model provides a useful tool to support the public health response to influenza and other infectious diseases.
Isothiocyanates induce UGT1A1 in humanized UGT1 mice in a CAR dependent fashion that is highly dependent upon oxidative stress
Authors: Yoda Emiko , Paszek Miles , Konopnicki Camille , Fujiwara Ryoichi , Chen Shujuan , Tukey Robert H.
Published: April 2017

Isothiocyanates, such as phenethyl isothiocyanate (PEITC), are formed following the consumption of cruciferous vegetables and generate reactive oxygen species (ROS) that lead to the induction of cytoprotective genes such as the UDP-glucuronosyltransferases (UGTs). The induction of ROS activates the Nrf2-Keap 1 pathway leading to the induction of genes through antioxidant response elements (AREs). UGT1A1, the sole enzyme responsible for the metabolism of bilirubin, can be induced following activation of Nrf2. When neonatal humanized UGT1 (hUGT1) mice, which exhibit severe levels of total serum bilirubin (TSB) because of a developmental delay in expression of the UGT1A1 gene, were treated with PEITC, TSB levels were reduced. Liver and intestinal UGT1A1 were induced, along with murine CYP2B10, a consensus CAR target gene. In both neonatal and adult hUGT1/Car−/− mice, PEITC was unable to induce CYP2B10. A similar result was observed following analysis of UGT1A1 expression in liver. However, TSB levels were still reduced in hUGT1/Car−/− neonatal mice because of ROS induction of intestinal UGT1A1. When oxidative stress was blocked by exposing mice to N-acetylcysteine, induction of liver UGT1A1 and CYP2B10 by PEITC was prevented. Thus, new findings in this report link an important role in CAR activation that is dependent upon oxidative stress.
Antioxidant synergistic effects of Osmanthus fragrans flowers with green tea and their major contributed antioxidant compounds
Authors: Mao Shuqin , Wang Kaidi , Lei Yukun , Yao Shuting , Lu Baiyi , Huang Weisu
Published: April 2017

The antioxidant synergistic effects of Osmanthus fragrans flowers with green tea were evaluated, and their major antioxidant compounds contributed to the total amount of synergy were determined. The antioxidant compounds in O. fragrans flowers with green tea were identified by LC-MS and quantified by UPLC-PDA. The synergistic antioxidant interactions between O. fragrans flowers with green tea and their antioxidant compounds were tested using the Prieto’s model after the simulated digestion. The main antioxidant compounds in O. fragrans flowers were acteoside and salideroside, whereas the main antioxidant compounds in green tea were caffeine, gallic acid, and L-epicatechin. The significant synergistic effect between O. fragrans flowers and green tea was observed and among nearly all of the combinations of their antioxidant compounds. Among the combinations, acteoside and gallic acid contributed most to the antioxidant synergy between O. fragrans flowers and green tea. However, the simulated digestion decreased this antioxidant synergy because it reduced the contents and the antioxidant capacities of their compounds, as well as the antioxidant synergy among the compounds.
In vitro biocompatibility study of sub-5 nm silica-coated magnetic iron oxide fluorescent nanoparticles for potential biomedical application
Authors: Foglia Sabrina , Ledda Mario , Fioretti Daniela , Iucci Giovanna , Papi Massimiliano , Capellini Giovanni , Lolli Maria Grazia , Grimaldi Settimio , Rinaldi Monica , Lisi Antonella
Published: April 2017

Magnetic iron oxide nanoparticles (IONPs), for their intriguing properties, have attracted a great interest as they can be employed in many different biomedical applications. In this multidisciplinary study, we synthetized and characterized ultrafine 3 nm superparamagnetic water-dispersible nanoparticles. By a facile and inexpensive one-pot approach, nanoparticles were coated with a shell of silica and contemporarily functionalized with fluorescein isothiocyanate (FITC) dye. The obtained sub-5 nm silica-coated magnetic iron oxide fluorescent (sub-5 SIO-Fl) nanoparticles were assayed for cellular uptake, biocompatibility and cytotoxicity in a human colon cancer cellular model. By confocal microscopy analysis we demonstrated that nanoparticles as-synthesized are internalized and do not interfere with the CaCo-2 cell cytoskeletal organization nor with their cellular adhesion. We assessed that they do not exhibit cytotoxicity, providing evidence that they do not affect shape, proliferation, cellular viability, cell cycle distribution and progression. We further demonstrated at molecular level that these nanoparticles do not interfere with the expression of key differentiation markers and do not affect pro-inflammatory cytokines response in Caco-2 cells. Overall, these results showed thein vitrobiocompatibility of the sub-5 SIO-Fl nanoparticles promising their safe employ for diagnostic and therapeutic biomedical applications.