bluuebunny/crossref_metadata_2025 huggingface dataset:
Northeastern American Forests: Natural Disturbances, Climate Change Impact, and the Utilization of Increasingly Damaged Forest Trees for Biofuel Production
Authors: Faluyi Marvellous Oluwaferanmi , Irmak Sibel
Published: December 2023

Forests and forestry-related industries and ecosystem services play a critical role in the daily life of all societies, including in cultural, ecological, social, economic, and environmental aspects. Globally, there are about 4.1 billion hectares of forestland. In the United States, there are about 304 million hectares of forestland, covering about 34% of the total land area, and the forest product industry produces over USD 200 billion worth of forestry products annually. Evidence suggests these precious resources may be negatively impacted by climate change via direct and indirect processes, including wildfires, insect/pest pressure, drought, extreme storm events, increased air temperature, solar radiation, vapor pressure deficit, and other factors and variables that can be detrimental. All these can not only cause significant changes in the health and productivity of the forests, but can also cause the extinction, migration, and/or re-distribution of different tree species. Thus, humankind has the paramount responsibility to take policy, technologic, economic, environmental, and management decisions and actions to protect this vital resource for current and future generations, plants, and animals. This paper provides an overview of some of the important characteristics of forest environmental services, climate–environment–forest interactions with respect to forest health and productivity, climate change’s impacts on forest species, and the utilization of forest biomass for high-value products.
Forest Visitors’ Multisensory Perception and Restoration Effects: A Study of China’s National Forest Parks by Introducing Generative Large Language Model
Authors: Wei Yu , Hou Yueyuan
Published: December 2023

Sensory perception of forests is closely related to human health and well-being. Based on attention recovery theory and stress relief theory, this paper investigates the influence of sensory perception of forests on visitors’ restoration effects from a multidimensional and multisensory perspective, integrating the use of a generative large language model, regression analysis, and semantic analysis. The results of the study show that (1) the application of a generative large language model provides new ideas and methods to solve the dilemma caused by the traditional self-report scale measurement and provides a possible way to explore a new research paradigm in the context of the rapid development of generative artificial intelligence; (2) the effects of each sensory quantity differed, with the sensory quantities of sight, hearing, touch, and taste having a significant positive effect on visitors’ restoration effects, and the sense of smell having a significant negative effect on visitors’ restoration effects; (3) sensory psychological distance partially had a significant effect on visitors’ restoration effects, both proximal psychological distance and distal psychological distance were significantly correlated with visitors’ restoration effects, and intermediate psychological distance had a negative effect on visitors’ restoration effects, but the effect was not significant; (4) the sensory dimension has a significant positive effect on visitors’ restoration effects, the integration and synergistic effect of the senses are enhanced, and multidimensional sensory cross-perception has a positive effect on visitors’ restoration effects at the social health level; and (5) the sensory elements of National Forest Parks that influence visitors’ restoration effects are mainly natural attributes, and the elements related to “people” also play an important role in visitors’ restoration effects. This study provides a useful complement to the study of forest sensory perception, and at the same time has an important reference value for exploring the management of forest recreation experience and sensory marketing practices.
PROFEE: A Probabilistic-Feedback Based Speed Rate Adaption for IEEE 802.11bc
Authors: Gomez Javier , Camacho-Escoto Jose Jaime , Orozco-Barbosa Luis , Rodriguez-Torres Diego
Published: December 2023

WiFi is a widely used wireless technology for data transmission. WiFi can also play a crucial role in simultaneously broadcasting content to multiple devices in multimedia transmission for venues such as classrooms, theaters, and stadiums, etc. Broadcasting allows for the efficient dissemination of information to all devices connected to the network, and it becomes crucial to ensure that the WiFi network has sufficient capacity to transmit broadcast multimedia content without interruptions or delays. However, using WiFi for broadcasting presents challenges that can impact user experience, specifically the difficulty of obtaining real-time feedback from potentially hundreds or thousands of users due to potential collisions of feedback messages. This work focuses on providing accurate feedback to the Access Point about the percentage of users not receiving broadcast traffic correctly so it can adjust its Modulation and Coding Scheme (MCS) while transmitting broadcast multimedia content to many users. The proposed method is comprised of two sequential algorithms. In order to reduce the probability of a collision after transmitting each message, an algorithm searches for the best probability value for users to transmit ACK/NACK messages, depending on whether messages are received correctly or not. This feedback allows the Access Point to estimate the number of STAs correctly/incorrectly receiving the messages being transmitted. A second algorithm uses this estimation so the Access Point can select the best MCS while maintaining the percentage of users not receiving broadcast content correctly within acceptable margins, thus providing users with the best possible content quality. We implemented the proposed method in the ns-3 simulator, and the results show it yields quick, reliable feedback to the Access Point that was then able to adjust to the best possible MCS in only a few seconds, regardless of the user density and dimensions of the scenario.
An Amphiphilic Multiblock Polymer as a High-Temperature Gelling Agent for Oil-Based Drilling Fluids and Its Mechanism of Action
Authors: He Yinbo , Du Mingliang , He Jing , Liu Haiyang , Lv Yanhua , Guo Lei , Zhang Peng , Bai Yunhai
Published: December 2023

Oil-based drilling fluids are widely used in challenging wells such as those with large displacements, deepwater and ultra-deepwater wells, deep wells, and ultra-deep wells due to their excellent temperature resistance, inhibition properties, and lubrication. However, there is a challenging issue of rheological deterioration of drilling fluids under high-temperature conditions. In this study, a dual-amphiphilic segmented high-temperature-resistant gelling agent (HTR-GA) was synthesized using poly fatty acids and polyether amines as raw materials. Experimental results showed that the initial decomposition temperature of HTR-GA was 374 °C, indicating good thermal stability. After adding HTR-GA, the emulsion coalescence voltage increased for emulsions with different oil-to-water ratios. HTR-GA could construct a weak gel structure in oil-based drilling fluids, significantly enhancing the shear-thinning and thixotropic properties of oil-based drilling fluids under high-temperature conditions. Using HTR-GA as the core, a set of oil-based drilling fluid systems with good rheological properties, a density of 2.2 g/cm3, and temperature resistance up to 220 °C were constructed. After aging for 24 h at 220 °C, the dynamic shear force exceeded 10 Pa, and G′ exceeded 7 Pa, while after aging for 96 h at 220 °C, the dynamic shear force exceeded 4 Pa, and G″ reached 7 Pa. The synthesized compound HTR-GA has been empirically validated to significantly augment the rheological properties of oil-based drilling fluids, particularly under high-temperature conditions, showcasing impressive thermal stability with a resistance threshold of up to 220 °C. This notable enhancement provides critical technical reinforcement for progressive exploration endeavors in deep and ultra-deep well formations, specifically employing oil-based drilling fluids.
Exploring Functionalized Magnetic Hydrogel Polyvinyl Alcohol and Chitosan Electrospun Nanofibers
Authors: Guerra Mónica , Garrudo Fábio F. F. , Faustino Célia , Rosa Maria Emilia , Ribeiro Maria H. L.
Published: December 2023

Nanofibrous materials present interesting characteristics, such as higher area/mass ratio and reactivity. These properties have been exploited in different applications, such as drug-controlled release and site-specific targeting of biomolecules for several disease treatments, including cancer. The main goal of this study was to develop magnetized nanofiber systems of lysozyme (Lys) for biological applications. The system envisaged electrospun polyvinyl alcohol (PVA) and PVA/chitosan (CS) nanofibers, loaded with Lys, crosslinked with boronic acids [phenylboronic acid (PBA), including 2-acetylphenylboronic acid (aPBA), 2-formylphenylboronic (fPBA), or bortezomib (BTZ)] and functionalized with magnetic nanobeads (IONPs), which was successfully built and tested using a microscale approach. Evaluation of the morphology of nanofibers, obtained by electrospinning, was carried out using SEM. The biological activities of the Lys-loaded PVA/CS (90:10 and 70:30) nanofibers were evaluated using the Micrococcus lysodeikticus method. To evaluate the success of the encapsulation process, the ratio of adsorbed Lys on the nanofibers, Lys activity, and in vitro Lys release were determined in buffer solution at pH values mimicking the environment of cancer cells. The viability of Caco-2 cancer cells was evaluated after being in contact with electrospun PVA + Lys and PVA/CS + Lys nanofibers, with or without boronic acid functionalation, and all were magnetized with IONPs.
Efficacy of Chitosan, Pectin and Xanthan as Cold Gelling Agents in Emulsion Gels Stabilized with Legume Proteins to Be Used as Pork Backfat Replacers in Beef Burgers
Authors: Cîrstea (Lazăr) Nicoleta , Nour Violeta , Corbu Alexandru Radu , Codină Georgiana Gabriela
Published: December 2023

This study aimed to develop stable emulsion gels enriched in polyunsaturated fatty acids, formulated with a mixture of olive (75%) and linseed (25%) oils, by incorporating two different stabilizers—pea and soy protein isolates—and three different cold gelling agents—chitosan, pectin and xanthan—to be used as pork backfat replacers in beef burgers. The color, pH, stability and textural properties of the emulsion gels were analyzed as affected by cold storage (4 °C, 7 days). Proximate composition, fatty acid content, technological and sensory properties were determined after burger processing. Meanwhile, color, pH, textural parameters and lipid oxidation were monitored in burgers at 0, 5 and 10 days of storage at 4 °C. A reduction of the fat content between 21.49% and 39.26% was achieved in the reformulated burgers as compared with the control, while the n-6/n-3 polyunsaturated fatty acid ratio decreased from 5.11 to 0.62. The highest moisture and fat retention were found in reformulated burgers made with xanthan, both with pea and soy proteins; however, their textural properties were negatively affected. The reformulated burgers made with chitosan were rated highest for sensory attributes and overall acceptability, not significantly different from the controls.
A Rare Case of Concurrent 2q34q36 Duplication and 2q37 Deletion in a Neonate with Syndromic Features
Authors: Riviello Francesco Nicola , Daponte Alessia , Ponzi Emanuela , Ficarella Romina , Orsini Paola , Bucci Roberta , Ventura Mario , Antonacci Francesca , Catacchio Claudia Rita , Gentile Mattia
Published: December 2023

Large-scale genomic structural variations can have significant clinical implications, depending on the specific altered genomic region. Briefly, 2q37 microdeletion syndrome is a prevalent subtelomeric deletion disorder characterized by variable-sized deletions. Affected patients exhibit a wide range of clinical manifestations, including short stature, facial dysmorphism, and features of autism spectrum disorder, among others. Conversely, isolated duplications of proximal chromosome 2q are rare and lack a distinct phenotype. In this report, we provide an extensive molecular analysis of a 15-day-old newborn referred for syndromic features. Our analysis reveals an 8.5 Mb microdeletion at 2q37.1, which extends to the telomere, in conjunction with an 8.6 Mb interstitial microduplication at 2q34q36.1. Our findings underscore the prominence of 2q37 terminal deletions as commonly reported genomic anomalies. We compare our patient’s phenotype with previously reported cases in the literature to contribute to a more refined classification of 2q37 microdeletion syndrome and assess the potential impact of 2q34q36.1 microduplication. We also investigate multiple hypotheses to clarify the genetic mechanisms responsible for the observed genomic rearrangement.
The Impact of Niclosamide Exposure on the Activity of Antioxidant Enzymes and the Expression of Glucose and Lipid Metabolism Genes in Black Carp (Mylopharyngodon piceus)
Authors: Wu Hao , Yuan Xiping , Xie Min , Gao Jinwei , Xiong Zhenzhen , Song Rui , Xie Zhonggui , Ou Dongsheng
Published: December 2023

Niclosamide (NIC, 2′,5-dichloro-4′-nitrosalicylanilide) is a salicylanilide molluscicide, and the extensive utilization and environmental pollution associated with NIC engender a potential hazard to both human health and the wellbeing of aquatic organisms. However, the mechanism of the chronic toxicity of NIC at environmentally relevant concentrations in terms of oxidative stress, metabolic disorder, and barrier functions in black carp (Mylopharyngodon piceus) is unknown. Therefore, healthy juvenile black carp (M. piceus) (average weight: 38.2 ± 2.5 g) were exposed to NIC at an environmentally realistic concentration (0, 10, and 50 μg/L) for 28 days. The findings of this study indicate that exposure to NIC resulted in reductions in weight gain, decreased activity of antioxidant enzymes, and increased expression of the Nrf2 gene. Furthermore, the liver demonstrated a greater accumulation of NIC than that in the gut and gills, as determined with a chemical analysis. Additionally, NIC exposure led to a significant reduction in ATP content and the activity of Na+/K+-ATPase and Ca2+/Mg2+-ATPase in the gut. Meanwhile, exposure to NIC resulted in a decrease in the liver glucose (Glu) level, gut cholesterol (CHO), and glycogen (Gln) and triglyceride (TG) content in all examined tissues. Conversely, it led to an increase in tissue lactic acid (LA) and acetyl-CoA levels, as well as LDH activity. Furthermore, NIC exposure at environmentally relevant concentrations demonstrated an upregulation in the expression of genes associated with glycolysis, such as PK and GK, while concurrently downregulating the gluconeogenesis gene G6Pase. Additionally, NIC exhibited an upregulation in the expression of genes related to β-oxidation, such as CPT1 and ACOX, while downregulating genes involved in triglyceride synthesis, including SREBP1, GPAT, FAS, and ACC1. Moreover, NIC facilitated fatty acid transportation through the overexpression of FATP and Fat/cd36. These results suggest that chronic exposure to NIC is associated with oxidative stress, compromised barrier function, and metabolic disorder. Moreover, these results underscore the significance of assessing the potential consequences of NIC for black carp and aquatic environments for aquaculture.
Characterization of the Complete Mitochondrial Genome and Phylogenetic Analyses of Eurytrema coelomaticum (Trematoda: Dicrocoeliidae)
Authors: Huang Fuqiang , Li Xin , Ye Bijin , Zhou Yule , Dang Zhisheng , Tang Wenqiang , Wang Long , Zhang Haoji , Chui Wenting , Kui Jun
Published: December 2023

Eurytrema coelomaticum, a pancreatic fluke, is recognized as a causative agent of substantial economic losses in ruminants. This infection, commonly referred to as eurytrematosis, is a significant concern due to its detrimental impact on livestock production. However, there is a paucity of knowledge regarding the mitochondrial genome of E. coelomaticum. In this study, we performed the initial sequencing of the complete mitochondrial genome of E. coelomaticum. Our findings unveiled that the mitochondrial genome of E. coelomaticum spans a length of 15,831 bp and consists of 12 protein-coding genes, 22 tRNA genes, two rRNA genes, and two noncoding regions. The A+T content constituted 62.49% of the genome. Moreover, all 12 protein-coding genes of E. coelomaticum exhibit the same arrangement as those of E. pancreaticum and other published species belonging to the family Dicrocoeliidae. The presence of a short string of additional amino acids (approximately 20~23 aa) at the N-terminal of the cox1 protein in both E. coelomaticum and E. pancreaticum mitochondrial genomes has contributed to the elongation of the cox1 gene in genus Eurytrema, surpassing that of all previously sequenced Dicrocoeliidae. The phylogenetic analysis displayed a close relationship between E. coelomaticum and E. pancreaticum, along with a genus-level association between Eurytrema and Lyperosomum. These findings underscore the importance of mitochondrial genomic data for comparative studies of Dicrocoeliidae and even Digenea, offering valuable DNA markers for future investigations in the systematic, epidemiological, and population genetic studies of this parasite and other digenean trematodes.
Mass-Transfer and Fluid Flow along Extensional Detachment Faults in Hyperextended Rift Systems: The Examples of Tasna in the Alps, Mauléon in the Pyrenees, and Hobby High Offshore Iberia
Authors: Pinto Victor Hugo Guimarães , Manatschal Gianreto , Karpoff Anne Marie , Masini Emmanuel , Victor Rodolfo Araújo , Viana Adriano Roessler , Ulrich Marc
Published: December 2023

Hyperextended rift systems are characterized by extreme crustal thinning and mantle exhumation associated with extensional detachment faults. These faults cut through thinned continental crust, reaching the underlying mantle and allowing for seawater to infiltrate and react with the crustal and mantle rocks. Hydrothermal fluid systems linked to detachment faults result in fluid–rock reactions occurring along the detachments, resulting in the breakdown and alteration of minerals, loss of elements and strain weakening in both mantle and crustal rocks. We present new geological observations and geochemical data from the modern Iberia and fossil Alpine Tethys Ocean Continent Transition and the West Pyrenean Mauléon hyperextended rift basin. We show evidence for a km-scale fluid flow along detachment faults and discuss the conditions under which fluid flow and mass transfer occurred. Convective fluid systems are of major importance for mass transfer between the mantle, crustal and marine reservoirs. We identified gains in Si, Mg, Fe, Mn, Ca, Ni, Cr and V along extensional detachment faults that we relate to channelized, hydrothermal crust- and mantle-reacted fluid systems migrating along detachments in the hyperextended continental crust. The observation that fault rocks of extensional detachment and syn-extensional sedimentary rocks are enriched in mantle-derived elements such as Cr, Ni and V enables us to define the pathways of fluids, as well as to estimate their age relative to detachment faulting and sedimentation. Because all three examples show a similar mass transport of elements along detachment systems at km-scale, we conclude that these examples are linked to convective fluid systems that may affect the thermal state of the lithosphere, as well as the rheology and chemistry of rocks in hyperextended systems, and may have implications for ore mineral exploration in hyperextended rift systems.
Characterization and Isolation of Piperamides from Piper nigrum Cultivated in Costa Rica
Authors: Vargas-Huertas Luis Felipe , Alvarado-Corella Luis Diego , Sánchez-Kopper Andrés , Araya-Sibaja Andrea Mariela , Navarro-Hoyos Mirtha
Published: December 2023

The piperamides profile of Piper nigrum cultivated in Costa Rica was studied using ultra-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight high-resolution mass spectrometry (UPLC-ESI-QTOF-HRMS) on enriched-piperamides extracts. A total of 31 different piperamides were identified, 24 of them with a methylenedioxyphenyl moiety, including piperine and nine other compounds with the characteristic piperidine ring, as well as guineensine, retrofractamide B, and eight other piperamides with an N-isobutyl group. In addition, piperyline and two other compounds with a pyrrolidine ring, as well as piperflaviflorine B, holding a N-2-methylbutyl chain, were characterized. In turn, pellitorine and six other piperamides exhibiting a long olefinic chain instead of the methylenedioxyphenyl group were also tentatively identified. In addition, quantification was performed using UPLC coupled with a diode array detector (UPLC-DAD), with 15 piperamides being quantified, including piperine, piperyline, piperanine, and piperloguminine with values within the range of previous reports, while results obtained for guineensine (276.5–421.0 mg/100 g dry material) and pellitorine (414.4–725.0 mg/100 g dry material) were higher than those reported in the literature. Additionally, preparative and semi-preparative high-performance liquid chromatography (HPLC) separations allowed to isolate, besides piperine, four other piperamides, which were identified through HRMS, 1H and 13C nuclear magnetic resonance (NMR). These included retrofractamide B, guineensine, pellitorine, and (2E,4E,12Z)-N-isobutyl-octadeca-2,4,12-trienamide, with yields of 134.0 mg/100 g dry material, 209.7 mg/100 g dry material, 361.8 mg/100 g dry material and 467.0 mg/100 g dry material, respectively, with all these values higher than those reported in previous studies in the literature. The findings constitute the first report of such a number and diversity of compounds in P. nigrum cultivated in Costa Rica.
Job Satisfaction and Associated Factors among Scrub Nurses: Beyond the Surface
Authors: Kurtović Biljana , Bilješko Štrus Ilijana
Published: December 2023

Background: Job satisfaction is essential, especially among healthcare professionals like scrub nurses, who often face unique professional challenges. This study aimed to evaluate job satisfaction and its related factors among scrub nurses, shedding light on areas of improvement and highlighting the positive aspects of their roles. Methods: A detailed study was conducted among a cohort of 31 scrub nurses using a 28-question survey. The questionnaire gauged various aspects of job satisfaction, from work conditions to professional growth opportunities. Results: Notably, the participants showed the least satisfaction with hazard allowances (1.8 ± 1.1) and break durations (1.9 ± 1.3). However, high satisfaction was observed regarding resource availability (3.9 ± 1.0) and the quality of protective equipment (3.7 ± 1.0). The data also revealed that those with 6–10 years of experience were the most satisfied (3.5), while those with 16–20 years were less content (2.7). Conclusion: The study indicates the need for improvements in hazard allowances and break periods. Experience significantly influenced satisfaction, with mid-career nurses showing the highest contentment. Such insights can guide future policy and practice adjustments in healthcare settings.
Correlations between Educational Struggle, Toxic Sites by School District and Demographic Variables, with Geographical Information System Projections
Authors: Shrestha Junu , Khan Raihan K. , McClintock Shane , DeGroote John , Zeman Catherine L.
Published: December 2023

This correlational study associated data on children enrolled in individualized educational plans in their K-12 schools (IEP) and an algorithm-calculated score of neurotoxins at contaminated sites located in each school district. The study also mapped and projected the correlations using Geographical Information System (GIS) technology. These data were populated in ArcMap 10.5 (a GIS software) for generating maps and data to conduct geospatial analysis. A total of 1 Superfund site and 39 CERCLA sites were identified as contaminated sites for this analysis. The majority of contaminants were heavy metals such as lead, arsenic, mercury, and cadmium. The mean toxic score of all contaminated sites combined was 13.4 (SD 14.4). Correlational analysis between the IEP numbers from each school district and toxic scores from the contaminated school district sites exhibited a positive relationship (F = 23.7, p < 0.0001). Correlations were also seen among higher toxics scores, IEP numbers, and children under the age of 10 (p < 0.00052) as well as higher proportions of black students in areas with high toxics scores (p = 0.0032). Black students were also far more likely to be enrolled in an IEP (p < 0.0001). Household income and poverty percentage in contaminated areas were also correlated (p = 0.0002). Individuals without college degrees were overrepresented in high toxic score school districts (p < 0.0001). The important low socio-economic status indicator of free and reduced lunch programs also correlated with increasing toxic scores (p = 0.0012) and IEP numbers (p = 0.0416). This study emphasizes the need to account for multiple exposures to wholistically appreciate environmental factors contributing to negative health outcomes.
An Aggregated Shape Similarity Index: A Case Study of Comparing the Footprints of OpenStreetMap and INSPIRE Buildings
Authors: Ďuračiová Renata
Published: December 2023

The mutual identification of spatial objects is a fundamental issue when updating geographic data with other data sets. Representations of spatial objects in different sources may not have the same identifiers, which would unambiguously assign them to each other. Intersections of spatial objects can be used for this purpose, but this does not allow for the detection of possible changes and their quantification. The aim of this paper is to propose a simple, applicable procedure for calculating the shape similarity measure, which should be able to efficiently identify different representations of spatial objects in two data sources, even though they may be changed or generalised. The main result is the aggregated index of shape similarity and instructions for its calculation and implementation. The shape similarity index is based on the calculation of the set similarity, the distance of the boundaries, and the differences in the area, perimeter, and number of the vertices of areal spatial objects. In the case study, the footprints of the building complexes in Dúbravka (part of the city of Bratislava, the capital of Slovakia) are compared using data from OpenStreetMap and INSPIRE (Infrastructure for Spatial Information in Europe) Buildings. A contribution to the quality check of the OpenStreetMap data is then a secondary result. The proposed method can be effectively used in the semi-automatic integration of heterogeneous data sources, updating the data source with other spatial data, or in their quality control.
Genome-Wide Identification and Expression Pattern Profiling of the Aquaporin Gene Family in Papaya (Carica papaya L.)
Authors: Zeng Qiuxia , Jia Haifeng , Ma Yaying , Xu Liangwei , Ming Ray , Yue Jingjing
Published: December 2023

Aquaporins (AQPs) are mainly responsible for the transportation of water and other small molecules such as CO2 and H2O2, and they perform diverse functions in plant growth, in development, and under stress conditions. They are also active participants in cell signal transduction in plants. However, little is known about AQP diversity, biological functions, and protein characteristics in papaya. To better understand the structure and function of CpAQPs in papaya, a total of 29 CpAQPs were identified and classified into five subfamilies. Analysis of gene structure and conserved motifs revealed that CpAQPs exhibited a degree of conservation, with some differentiation among subfamilies. The predicted interaction network showed that the PIP subfamily had the strongest protein interactions within the subfamily, while the SIP subfamily showed extensive interaction with members of the PIP, TIP, NIP, and XIP subfamilies. Furthermore, the analysis of CpAQPs’ promoters revealed a large number of cis-elements participating in light, hormone, and stress responses. CpAQPs exhibited different expression patterns in various tissues and under different stress conditions. Collectively, these results provided a foundation for further functional investigations of CpAQPs in ripening, as well as leaf, flower, fruit, and seed development. They also shed light on the potential roles of CpAQP genes in response to environmental factors, offering valuable insights into their biological functions in papaya.
Current Status and Molecular Mechanisms of Resistance to Immunotherapy in Oral Malignant Melanoma
Authors: Usta Sena Zeynep , Uchihashi Toshihiro , Kodama Shingo , Kurioka Kyoko , Inubushi Toshihiro , Shimooka Takuya , Sugauchi Akinari , Seki Soju , Tanaka Susumu
Published: December 2023

Immune checkpoint inhibitors (ICIs), including anti-cytotoxic T-lymphocyte-associated protein 4 (CTLA-4) and anti-programmed death-1 (PD-1) antibodies, have initiated a new era in the treatment of malignant melanoma. ICIs can be used in various settings, including first-line, adjuvant, and neo-adjuvant therapy. In the scope of this review, we examined clinical studies utilizing ICIs in the context of treating oral mucosal melanoma, a rare disease, albeit with an extremely poor prognosis, with a specific focus on unraveling the intricate web of resistance mechanisms. The absence of a comprehensive review focusing on ICIs in oral mucosal melanoma is notable. Therefore, this review seeks to address this deficiency by offering a novel and thorough analysis of the current status, potential resistance mechanisms, and future prospects of applying ICIs specifically to oral malignant melanoma. Clarifying and thoroughly understanding these mechanisms will facilitate the advancement of effective therapeutic approaches and enhance the prospects for patients suffering from oral mucosal melanoma.
Genetic Variability in VEGFA Gene Influences the Effectiveness of Tennis Elbow Therapy with PRP: A Two-Year Prospective Cohort Study
Authors: Niemiec Paweł , Jarosz Alicja , Balcerzyk-Matić Anna , Iwanicka Joanna , Nowak Tomasz , Iwanicki Tomasz , Gierek Marcin , Kalita Marcin , Garczorz Wojciech , Francuz Tomasz , Górczyńska-Kosiorz Sylwia , Kania Wojciech , Szyluk Karol
Published: December 2023

Vascular endothelial growth factor (VEGF) is implicated in both the etiology of tendinopathy and its healing process. Polymorphic variants of the VEGFA gene exhibit varied expression, which can influence the phenotype and treatment effectiveness. The aim of the present study was to analyze the influence of VEGFA gene variants on the effectiveness of tennis elbow therapy using platelet-rich plasma (PRP), measured through common patient-reported outcome measures (PROMs). A cohort of 107 patients (132 elbows) with tennis elbow was prospectively analyzed, with a two-year follow-up (at weeks 2, 4, 8, 12, 24, 52, and 104 after PRP injection). PROMs values were compared between variants of five VEGFA gene polymorphisms (rs699947 A>C, rs2010963 C>G, rs1413711 C>T, rs3024998 C>T and rs3025021 C>T) at each follow-up point. Patients with genotypes GG (rs2010963) and CC (rs3024998) had better response to PRP therapy (significantly fewer symptoms and limitations in the upper limb compared to carriers of alleles C and T, respectively). Polymorphisms influenced also selected hematological parameters. VEGFA gene polymorphisms (rs2010963 and rs3024998) appear to be significant treatment modifiers for tendinopathy, and their genotyping may serve as an effective tool for personalized patient selection for PRP therapy.
Blood and Brain Metabolites after Cerebral Ischemia
Authors: Baranovicova Eva , Kalenska Dagmar , Kaplan Peter , Kovalska Maria , Tatarkova Zuzana , Lehotsky Jan
Published: December 2023

The study of an organism’s response to cerebral ischemia at different levels is essential to understanding the mechanism of the injury and protection. A great interest is devoted to finding the links between quantitative metabolic changes and post-ischemic damage. This work aims to summarize the outcomes of the most studied metabolites in brain tissue—lactate, glutamine, GABA (4-aminobutyric acid), glutamate, and NAA (N-acetyl aspartate)—regarding their biological function in physiological conditions and their role after cerebral ischemia/reperfusion. We focused on ischemic damage and post-ischemic recovery in both experimental—including our results—as well as clinical studies. We discuss the role of blood glucose in view of the diverse impact of hyperglycemia, whether experimentally induced, caused by insulin resistance, or developed as a stress response to the cerebral ischemic event. Additionally, based on our and other studies, we analyze and critically discuss post-ischemic alterations in energy metabolites and the elevation of blood ketone bodies observed in the studies on rodents. To complete the schema, we discuss alterations in blood plasma circulating amino acids after cerebral ischemia. So far, no fundamental brain or blood metabolite(s) has been recognized as a relevant biological marker with the feasibility to determine the post-ischemic outcome or extent of ischemic damage. However, studies from our group on rats subjected to protective ischemic preconditioning showed that these animals did not develop post-ischemic hyperglycemia and manifested a decreased metabolic infringement and faster metabolomic recovery. The metabolomic approach is an additional tool for understanding damaging and/or restorative processes within the affected brain region reflected in the blood to uncover the response of the whole organism via interorgan metabolic communications to the stressful cerebral ischemic challenge.
Vitiligo: Pathogenesis and New and Emerging Treatments
Authors: Perez-Bootello Javier , Cova-Martin Ruth , Naharro-Rodriguez Jorge , Segurado-Miravalles Gonzalo
Published: December 2023

Vitiligo is a complex disease with a multifactorial nature and a high impact on the quality of life of patients. Although there are multiple therapeutic alternatives, there is currently no fully effective treatment for this disease. In the current era, multiple drugs are being developed for the treatment of autoimmune diseases. This review assesses the available evidence on the pathogenesis of vitiligo, and a comprehensive review of treatments available for vitiligo now and in the near future is provided. This qualitative analysis spans 116 articles. We reviewed the mechanism of action, efficacy and safety data of phototherapy, afamelanotide, cyclosporine, phosphodiesterase 4 inhibitors, trichloroacetic acid, basic fibroblast growth factor, tumor necrosis factor (TNF) inhibitors, secukinumab, pseudocatalase and janus kinase (JAK) inhibitors. At the moment, there is no clearly outstanding option or fully satisfactory treatment for vitiligo, so it is necessary to keep up the development of new drugs as well as the publication of long-term effectiveness and safety data for existing treatments.
Altered Phenotypes of Breast Epithelial × Breast Cancer Hybrids after ZEB1 Knock-Out
Authors: Merckens Alexander , Sieler Mareike , Keil Silvia , Dittmar Thomas
Published: December 2023

ZEB1 plays a pivotal role in epithelial-to-mesenchymal transition (EMT), (cancer) cell stemness and cancer therapy resistance. The M13HS tumor hybrids, which were derived from spontaneous fusion events between the M13SV1-EGFP-Neo breast epithelial cells and HS578T-Hyg breast cancer cells, express ZEB1 and exhibit prospective cancer stem cell properties. To explore a possible correlation between the ZEB1 and stemness/ EMT-related properties in M13HS tumor hybrids, ZEB1 was knocked-out by CRISPR/Cas9. Colony formation, mammosphere formation, cell migration, invasion assays, flow cytometry and Western blot analyses were performed for the characterization of ZEB1 knock-out cells. The ZEB1 knock-out in M13HS tumor cells was not correlated with the down-regulation of the EMT-related markers N-CADHERIN (CDH2) and VIMENTIN and up-regulation of miR-200c-3p. Nonetheless, both the colony formation and mammosphere formation capacities of the M13HS ZEB1 knock-out cells were markedly reduced. Interestingly, the M13HS-2 ZEB1-KO cells harbored a markedly higher fraction of ALDH1-positive cells. The Transwell/ Boyden chamber migration assay data indicated a reduced migratory activity of the M13HS ZEB1-knock-out tumor hybrids, whereas in scratch/ wound-healing assays only the M13SH-8 ZEB1-knock-out cells possessed a reduced locomotory activity. Similarly, only the M13HS-8 ZEB1-knock-out tumor hybrids showed a reduced invasion capacity. Although the ZEB1 knock-out resulted in only moderate phenotypic changes, our data support the role of ZEB1 in EMT and stemness.
Biosynthesis of the Inner Core of Bordetella pertussis Lipopolysaccharides: Effect of Mutations on LPS Structure, Cell Division, and Toll-like Receptor 4 Activation
Authors: Pérez-Ortega Jesús , van Boxtel Ria , Plisnier Michel , Ingels Dominique , Devos Nathalie , Sijmons Steven , Tommassen Jan
Published: December 2023

Previously developed whole-cell vaccines against Bordetella pertussis, the causative agent of whooping cough, appeared to be too reactogenic due to their endotoxin content. Reduction in endotoxicity can generally be achieved through structural modifications in the lipid A moiety of lipopolysaccharides (LPS). In this study, we found that dephosphorylation of lipid A in B. pertussis through the heterologous production of the phosphatase LpxE from Francisella novicida did, unexpectedly, not affect Toll-like receptor 4 (TLR4)-stimulating activity. We then focused on the inner core of LPS, whose synthesis has so far not been studied in B. pertussis. The kdtA and kdkA genes, responsible for the incorporation of a single 3-deoxy-D-manno-oct-2-ulosonic acid (Kdo) residue in the inner core and its phosphorylation, respectively, appeared to be essential. However, the Kdo-bound phosphate could be replaced by a second Kdo after the heterologous production of Escherichia coli kdtA. This structural change in the inner core affected outer-core and lipid A structures and also bacterial physiology, as reflected in cell filamentation and a switch in virulence phase. Furthermore, the eptB gene responsible for the non-stoichiometric substitution of Kdo-bound phosphate with phosphoethanolamine was identified and inactivated. Interestingly, the constructed inner-core modifications affected TLR4-stimulating activity. Whereas endotoxicity studies generally focus on the lipid A moiety, our data demonstrate that structural changes in the inner core can also affect TLR4-stimulating activity.
Electrophoretically Co-Deposited Collagen–Lactoferrin Membranes with Enhanced Pro-Regenerative Properties for Oral Soft Tissue Regeneration
Authors: Antoshin Artem , Gostev Mikhail , Khristidis Yana , Giliazova Aliia , Voloshin Sergei , Blagushina Nataliia , Smirnova Olga , Diachkova Ekaterina , Istranova Elena , Usanova Anna , Solodov Nikolai , Fayzullin Alexey , Ivanova Elena , Sadchikova Elena , Vergara Bashkatova Milena Noelia , Drakina Olga , Tarasenko Svetlana , Timashev Peter
Published: December 2023

The quality of soft tissue defect regeneration after dental surgeries largely determines their final success. Collagen membranes have been proposed for the healing of such defects, but in some cases, they do not guarantee a sufficient volume of the regenerated tissue and vascularization. For this purpose, lactoferrin, a protein with natural pro-regenerative, anti-inflammatory, and pro-angiogenic activity, can be added to collagen. In this article, we used a semipermeable barrier-assisted electrophoretic deposition (SBA-EPD) method for the production of collagen–lactoferrin membranes. The membrane structure was studied by SEM, and its mechanical properties were shown. The lactoferrin release kinetics were shown by ELISA within 75 h. When tested in vitro, we demonstrated that the collagen–lactoferrin membranes significantly increased the proliferation of keratinocytes (HaCaT) and fibroblasts (977hTERT) compared to blank collagen membranes. In vivo, on the vestibuloplasty and free gingival graft harvesting models, we showed that collagen–lactoferrin membranes decreased the wound inflammation and increased the healing rates and regeneration quality. In some parameters, collagen–lactoferrin membranes outperformed not only blank collagen membranes, but also the commercial membrane Mucograft®. Thus, we proved that collagen–lactoferrin membranes produced by the SBA-EPD method may be a valuable alternative to commercially used membranes for soft tissue regeneration in the oral cavity.
RpoS-Regulated Genes and Phenotypes in the Phytopathogenic Bacterium Pectobacterium atrosepticum
Authors: Petrova Olga , Semenova Elizaveta , Parfirova Olga , Tsers Ivan , Gogoleva Natalia , Gogolev Yuri , Nikolaichik Yevgeny , Gorshkov Vladimir
Published: December 2023

The alternative sigma factor RpoS is considered to be one of the major regulators providing stress resistance and cross-protection in bacteria. In phytopathogenic bacteria, the effects of RpoS have not been analyzed with regard to cross-protection, and genes whose expression is directly or indirectly controlled by RpoS have not been determined at the whole-transcriptome level. Our study aimed to determine RpoS-regulated genes and phenotypes in the phytopathogenic bacterium Pectobacterium atrosepticum. Knockout of the rpoS gene in P. atrosepticum affected the long-term starvation response, cross-protection, and virulence toward plants with enhanced immune status. The whole-transcriptome profiles of the wild-type P. atrosepticum strain and its ΔrpoS mutant were compared under different experimental conditions, and functional gene groups whose expression was affected by RpoS were determined. The RpoS promoter motif was inferred within the promoter regions of the genes affected by rpoS deletion, and the P. atrosepticum RpoS regulon was predicted. Based on RpoS-controlled phenotypes, transcriptome profiles, and RpoS regulon composition, the regulatory role of RpoS in P. atrosepticum is discussed.
Combining Transcriptome and Whole Genome Re-Sequencing to Screen Disease Resistance Genes for Wheat Dwarf Bunt
Authors: Jia Yufeng , Shen Tong , Wen Zhiwei , Chen Jing , Liu Qi
Published: December 2023

Wheat dwarf bunt is a damaging disease caused by Tilletia controversa Kühn (TCK). Once the disease infects wheat, it is difficult to control and will significantly reduce wheat output and quality. RNA sequencing and whole genome re-sequencing were used to search for potential TCK resistance genes in Yili 053 (sensitive variety) and Zhongmai 175 (moderately resistant variety) in the mid-filling, late-filling, and maturity stages. The transcriptomic analysis revealed 11 potential disease resistance genes. An association analysis of the findings from re-sequencing found nine genes with single nucleotide polymorphism mutations. The Kyoto Encyclopedia of Genes and Genomes enrichment analysis showed that three up-regulated genes were involved in the synthesis of benzoxazinone and tryptophan metabolism. Additionally, quantitative real-time polymerase chain reaction confirmed the RNA sequencing results. The results revealed novel TCK resistance genes and provide a theoretical basis for researching the function of resistance genes and molecular breeding.
Fisetin Inhibits UVA-Induced Expression of MMP-1 and MMP-3 through the NOX/ROS/MAPK Pathway in Human Dermal Fibroblasts and Human Epidermal Keratinocytes
Authors: Jang Hye-Yeon , Kim Gi-Beum , Kim Jeong-Mi , Kang Sang Yull , Youn Hyun-Jo , Park Jinny , Ro Su Yeon , Chung Eun-Yong , Park Kwang-Hyun , Kim Jong-Suk
Published: December 2023

Fisetin is a flavonoid found in plants and has been reported to be effective in various human diseases. However, the effective mechanisms of ultraviolet-A (UVA)-mediated skin damage are not yet clear. In this study, we investigated the protective mechanisms of fisetin regarding UVA-induced human dermal fibroblasts (HDFs) and human epidermal keratinocytes (HEKs) damages. Fisetin showed a cytoprotective effect against UVA irradiation and suppressed matrix metalloproteinases (MMPs), MMP-1, and MMP-3 expression. In addition, fisetin was rescued, which decreased mRNA levels of pro-inflammatory cytokines, reactive oxygen species production, and the downregulation of MAPK/AP-1 related protein and NADPH oxidase (NOX) mRNA levels. Furthermore, UVA-induced MMP-1 and MMP-3 were effectively inhibited by siRNAs to NOX 1 to 5 in HDFs and HEKs. These results indicate that fisetin suppresses UVA-induced damage through the NOX/ROS/MAPK pathway in HDFs and HEKs.
Gated Convolution and Stacked Self-Attention Encoder–Decoder-Based Model for Offline Handwritten Ethiopic Text Recognition
Authors: Tadesse Direselign Addis , Liu Chuan-Ming , Ta Van-Dai
Published: December 2023

Offline handwritten text recognition (HTR) is a long-standing research project for a wide range of applications, including assisting visually impaired users, humans and robot interactions, and the automatic entry of business documents. However, due to variations in writing styles, visual similarities between different characters, overlap between characters, and source document noise, designing an accurate and flexible HTR system is challenging. The problem becomes serious when the algorithm has a low learning capacity and when the text used is complex and has a lot of characters in the writing system, such as Ethiopic script. In this paper, we propose a new model that recognizes offline handwritten Ethiopic text using a gated convolution and stacked self-attention encoder–decoder network. The proposed model has a feature extraction layer, an encoder layer, and a decoder layer. The feature extraction layer extracts high-dimensional invariant feature maps from the input handwritten image. Using the extracted feature maps, the encoder and decoder layers transcribe the corresponding text. For the training and testing of the proposed model, we prepare an offline handwritten Ethiopic text-line dataset (HETD) with 2800 samples and a handwritten Ethiopic word dataset (HEWD) with 10,540 samples obtained from 250 volunteers. The experiment results of the proposed model on HETD show a 9.17 and 13.11 Character Error Rate (CER) and Word Error Rate (WER), respectively. However, the model on HEWD shows an 8.22 and 9.17 CER and WER, respectively. These results and the prepared datasets will be used as a baseline for future research.