bluuebunny/crossref_metadata_2025 huggingface dataset:
Population Structure Analysis and Association Mapping for Turcicum Leaf Blight Resistance in Tropical Maize Using SSR Markers
Authors: Kumar Bhupender , Choudhary Mukesh , Kumar Pardeep , Kumar Krishan , Kumar Sonu , Singh Brijesh Kumar , Lahkar Chayanika , Meenakshi  , Kumar Pushpendra , Dar Zahoor Ahmed , Devlash Rakesh , Hooda Karambir Singh , Guleria Satish Kumar , Rakshit Sujay
Published: March 2022

Maize is an important cereal crop in the world for feed, food, fodder, and raw materials of industries. Turcicum leaf blight (TLB) is a major foliar disease that can cause more than 50% yield losses in maize. Considering this, the molecular diversity, population structure, and genome-wide association study (GWAS) for TLB resistance were studied in 288 diverse inbred lines genotyped using 89 polymorphic simple sequence repeats (SSR) markers. These lines werescreened for TLB disease at two hot-spot locations under artificially inoculated conditions. The average percent disease incidence (PDI) calculated for each genotype ranged from 17 (UMI 1201) to 78% (IML 12-22) with an overall mean of 40%. The numbers of alleles detected at a locus ranged from twoto nine, with a total of 388 alleles. The polymorphic information content (PIC) of each marker ranged between 0.04 and 0.86. Out of 89 markers, 47 markers were highly polymorphic (PIC ≥ 0.60). This indicated that the SSR markers used were very informative and suitable for genetic diversity, population structure, and marker-trait association studies.The overall observed homozygosity for highly polymorphic markers was 0.98, which indicated that lines used were genetically pure. Neighbor-joining clustering, factorial analysis, and population structure studies clustered the 288 lines into 3–5 groups. The patterns of grouping were in agreement with the origin and pedigree records of the genotypesto a greater extent.A total of 94.10% lines were successfully assigned to one or another group at a membership probability of ≥0.60. An analysis of molecular variance (AMOVA) revealed highly significant differences among populations and within individuals. Linkage disequilibrium for r2 and D′ between loci ranged from 0 to 0.77 and 0 to 1, respectively. A marker trait association analysis carried out using a general linear model (GLM) and mixed linear model (MLM), identified 15 SSRs markers significantly associated with TLB resistance.These 15 markers were located on almost all chromosomes (Chr) except 7, 8, and 9. The phenotypic variation explained by these loci ranged from 6% (umc1367) to 26% (nc130, phi085). Maximum 7 associated markers were located together on Chr 2 and 5. The selected regions identified on Chr 2 and 5 corroborated the previous studies carried out in the Indian maize germplasm. Further, 11 candidate genes were identified to be associated with significant markers. The identified sources for TLB resistance and associated markers may be utilized in molecular breeding for the development of suitable genotypes.
Korean Version of the 17-Item Utrecht Work Engagement Scale for University Students: A Validity and Reliability Study
Authors: Jang Aeri , An Minjeong
Published: March 2022

Work engagement is a factor that has key influence on learning outcomes for college students. The psychometric properties of the Korean version of the 17-item Utrecht work engagement scale for students (UWES-S) survey have yet to be determined. The purpose of this study was to test the reliability and validity of the Korean version of the 17-item UWES-S among Korean college students. A total of 248 college students were recruited from three universities in South Korea. Data were analyzed using IBM SPSS and the Mplus program. Among 248 college students, the mean age was 21.19 years, and 87.5% of the students were female. Reliability was deemed satisfactory by Cronbach’s alpha 0.940, McDonald’s omega 0.941, and composite reliability 0.941. Construct validity was supported by confirmatory factor analysis results (CFI = 0.93, TLI = 0.91, SRMR = 0.05, RMSEA = 0.08). Convergent validity was supported by the significant relationship between academic engagement and burnout (r = −0.344, p < 0.001). Our findings showed that the Korean version of the 17-item UWES-S was a reliable and valid instrument. This instrument can be used to assess and improve work engagement in college students.
Reproductive Issues in Long-Term Surviving Patients following Therapy for Hodgkin’s Disease in the Republic of North Macedonia: Risks of Infertility According to First-Line Treatment Regimens
Authors: Amzai Gazmend , Karanfilski Oliver , Stavrikj Sonja Genadieva , Stojanovikj Aleksandar
Published: March 2022

Infertility as a consequence of therapy presents a high psychosocial burden for HL patients. In the cohort of our analyzed patients, within the post-ABVD surviving patients, alterations of the spermogram were documented in a total of 6.1% of the male patients and 5.4% of the female patients developed amenorrhea. On the other hand, within the subgroup of surviving patients following BEACOPP chemotherapy, 60% of the male patients manifested defects in their spermogram, and as high as 28.6% of the female survivors reported loss of their monthly cycle. It has been reported on several occasions that even prior to treatment, the sperm of HL patients manifests poorer quality characteristics when analyzed against control specimens from healthy male donors. The analyzed results in ABVD-treated male HL patients confirm ABVD to be a safe regimen for males of all age categories, as well as for female patients under the age of thirty. In women above the age of 30, the infertility risk rate is relatively low (14%), which leaves the decision of preserving fertility to themselves. For all BEACOPP-treated female, as well as male patients, a consult with a reproductive medicine specialist is warranted prior to therapy, due to the high infertility risk, and the final decision should be made on an individual basis.
A Study of Shoot Growth, Leaf Photosynthesis, and Nutrients in ‘Lingfengjing’ Litchi Grafted onto Seedlings of Different Cultivars
Authors: Fan Yan , Li Zhiyuan , Xie Binxia , Liang Xiaowen , Huang Xuming
Published: March 2022

Litchi (Litchi chinensis Sonn.) is one of the important fruit crops in southern China. ‘Lingfengnuo’ (LFN) is a new late-maturing litchi variety which has gained great popularity among consumers and growers due to its high quality and reliable productivity. However, there has been limited research on the graft compatibility of LFN on different rootstocks, which is important for selecting the optimal rootstocks for propagation, and thus application of this new cultivar. In this study, LFN scions were grafted onto one-year-old seedlings from 13 cultivars including ‘Heiye’ (HY), ‘Shakeng’ (SK), ‘Hehuadahongli’ (HHDHL), ‘Maguili’ (MGL), ‘Xiaojinzhong’ (XJZ), ‘Huaizhi’ (HZ), ‘Chenzi’ (CZ), ‘Shangshuhuai’ (SSH), ‘Baitangying’ (BTY), ‘Shuangjianyuhebao’ (SJYHB), ‘Jingganghongnuo’ (JGHN), ‘Baila’ (BL), and ‘Shuidong’ (SD). Graft success, morphology of the graft joint, shoot growth, leaf photosynthetic rate, and nutrients were compared. Graft success was highest for XJZ, HZ, BL and JGHN. Tree vigor, reflected by flush growth, was highest for HZ, followed by JGHN and BTY, but weakest for HY, SD, SJYHB, and XJZ. The stem significantly swelled above the graft joint in trees with rootstocks of HY, SD, and SJYHB seedlings; however, this did not occur in XJZ. Leaf photosynthesis displayed a similar pattern to tree vigor, and was highest for HZ and lowest for HY, SD, and XJZ. These low vigor trees produced flowers the next year after grafting. The result suggested that HZ, JGHN, and BTY seedlings as rootstock are highly compatible with LFN, while those of HY, SD, and SJYHB are poorly compatible. Rootstocks did not significantly influence the leaf N, P, and Fe, but showed significant differences in other minerals. The leaf Ca, Mg, Zn and B were lowest in the trees graft onto HZ seedlings. Based on these results, HZ seedlings are recommended as the best rootstock for the propagation of LFN; however, a supply of Ca, Mg, B and Zn fertilizers a especially needed for LFN trees on HZ seedling rootstock.
Preliminary Studies on Suppression of Important Plant Pathogens by Using Pomegranate and Avocado Residual Peel and Seed Extracts
Authors: Leontopoulos Stefanos , Skenderidis Prodromos , Petrotos Konstantinos , Mitsagga Chrysanthi , Giavasis Ioannis
Published: March 2022

Potential synergistic action of aqueous extracts of pomegranate peel (PP), avocado peel (AP), and avocado seed (AS) wastes isolated by microwave-assisted extraction were assessed in in vitro and in vivo assays as biocontrol agents against several plant pathogenic fungi. The study findings contribute to the utilization of a value-added industrial byproduct and provide significant value in advancing the development of new plant protecting compositions that benefit from the synergistic effects between two important plant species that contain several natural bioactive compounds. More specifically, the in vitro results proved that the use of 100%-pure (PP) extracted waste affected the mycelium growth of Penicillium expansum. Furthermore, mycelium growth of Aspergillus niger was decreased by 10.21% compared to control after 7 days of growth in medium agar containing 100% AP and extracted waste. Moreover, mycelium growth of Botrytis cinerea was affected by equal volume of avocado extraction wastes (50% peel and 50% seed) only at the first 3 days of the inoculation, while at the seventh day of the inoculation there was no effect on the mycelium growth. Equal volumes of the examined wastes showed decreased mycelium growth of Fusarium oxysporum f.sp. lycopersici by 6%, while Rhizoctonia solani mycelium growth was found to be the most sensitive in PP application. In addition, the in vivo assay shown that PP extract suppresses damage of tomato plants caused by R. solani followed by extracted wastes from AP. Based on the research findings, it can be argued that PP and AP extracts can be used as natural antifungals instead of dangerous synthetic antifungals to effectively treat phytopathogens that cause fruit and vegetable losses during cultivation.
Optimum Sterilization Method for In Vitro Cultivation of Dimorphic Seeds of the Succulent Halophyte Suaeda aralocaspica
Authors: Si Yu , Haxim Yakupjan , Wang Lei
Published: March 2022

Suaeda aralocaspica is an annual halophyte in the Amaranthaceae in the saline deserts of central Asia. This plant has succulent leaves and grape-like fruits and is a potential horticultural plant. To obtain the efficient sterilization method and optimal culture conditions, two types of seeds produced from a single plant of S. aralocaspica were treated with 75% ethanol for different time durations first, and then sodium hypochlorite (NaClO) or mercury chloride (HgCl2), with five different timing treatments were used for second seed surface sterilization. Sterilized seeds were germinated on a Murashige and Skoog (MS) medium at different potential hydrogenation (pH) levels, to examine germination and seedling performance. The results showed that the highest germination percentage of brown seeds was 100% and that of black seeds was 17%. Thus, brown seeds were more suitable for further culture experiments than black seeds. For brown seeds, the sterilization effect of NaClO was better than that of HgCl2, based on the results of seed germination, contamination, and seedling survival. Rinsing with 75% ethanol for 60 s, sterilizing with NaClO for 8 min, and cultivating at pH 8.0 MS for 7 days was the best of all sterilization procedures and cultivation methods tested, which has been successfully applied to S. aralocaspica in vitro culture. The optimized protocol described here can be used as the reference for the Suaeda genus.
Successful Pre-Rewarming Resuscitation after Cardiac Arrest in Severe Hypothermia: A Retrospective Cohort Study from the International Hypothermia Registry
Authors: Cools Evelien , Meyer Marie , Courvoisier Delphine , Walpoth Beat
Published: March 2022

The aim of our study is to investigate successful pre-rewarming resuscitation after hypothermic cardiac arrest (HCA). The hypothermic heart may be insensitive to defibrillation when core temperature is below 30 °C and after successful defibrillation, sinus rhythm often returns into ventricular fibrillation. Recurrent defibrillation attempts may induce myocardial injury. Discrepancy exists concerning pre-rewarming defibrillation between the guidelines of the European Resuscitation Council and American Heart Association. The International Hypothermia Registry (IHR) gathers hypothermia cases. The primary outcome was survival. Secondary outcomes were the characteristics of defibrillation, the effect of Adrenaline administration under 30 °C, and the duration of CPR. Of the 239 patients, eighty-eight were in cardiac arrest at arrival of the rescue team. Successful pre-rewarming resuscitation was obtained in 14 patients. The outcome showed: seven deaths, one vegetative state, two patients with reversible damage, and four patients with full recovery. A total of five patients had a shockable rhythm, and defibrillation was successful in four patients. The response rate to Adrenaline was reported as normal in six patients. There were no statistically significant differences in the presence of a shockable rhythm, the success of defibrillation, and the effect on Adrenaline administration between the survivors and non-survivors. Successful resuscitation in severe hypothermia is possible before active rewarming and arrival in the hospital, thus improving the chance of survival.
Plasma Biology 2.0
Authors: Sakudo Akikazu , Yagyu Yoshihito
Published: March 2022

Plasma biology is a cutting-edge research field that involves plasma technology [...]
Need and Viability of Newborn Screening Programme in India: Report from a Pilot Study
Authors: Raveendran Arya , Chacko Teena Joseph , Prabhu Priya , Varma Raghava , Lewis Leslie Edward , Rao Pragna , Shetty Prajna P. , Mallimoggala Yajna S. Phaneendra , Hedge Asha , Nayak Dinesh M. , Moorkoth Sudeep , Moorkoth Sudheer
Published: March 2022

India, a country with the second largest population in the world, does not have a national newborn screening programme as part of its health policy. With funding support from the Grand Challenges Canada, a pilot newborn screening programme was implemented for the Udupi district of South India to study the need and viability of a national programme in India. Six disorders were selected for the study based on the availability of funding and recommendation from pediatricians in the district. Here, we report the observed incidence during the study. A cost-effectiveness analysis of implementing newborn screening in India was performed. It is evident from our analysis that the financial loss for the nation due to these preventable diseases is much higher than the overall expenditure for screening, diagnosis, and treatment. This cost-effectiveness analysis justifies the need for a national newborn screening programme in India.
Increasing Frequency of G275E Mutation in the Nicotinic Acetylcholine Receptor α6 Subunit Conferring Spinetoram Resistance in Invading Populations of Western Flower Thrips in China
Authors: Sun Li-Na , Shen Xiu-Jing , Cao Li-Jun , Chen Jin-Cui , Ma Li-Jun , Wu San-An , Hoffmann Ary , Wei Shu-Jun
Published: March 2022

The western flower thrips Frankliniella occidentalis (Pergande) (Thysanoptera: Thripidae) is an important invasive pest worldwide. Field-evolved resistance to the pesticide spinetoram is an increasing problem in the chemical control of this pest. Here, we examined changes in the frequency of a genetic mutation associated with spinetoram resistance, the G275E mutation in the acetylcholine receptor Foα6, in 62 field populations collected from 2009 to 2021 across areas of China invaded by this pest. We found a low frequency of the G275E mutation in populations collected at the early invasion stage, in contrast to a high frequency in native USA populations. However, the frequency of the G275E mutation has increased to a high level in recently collected populations, with the mutation becoming fixed in some populations. There was a correlation between the frequency of the G275E mutation and resistance to spinetoram as characterized by median lethal concentration, although two populations were outliers. These results showed that G275E mutation is one of the mechanisms conferring spinetoram resistance in many invading populations in China. Ongoing dispersal of the WFT may have facilitated a rapid increase in the G275E mutation across China. Our study highlights the rapid evolution of pesticide resistance in an invasive species and points to a useful marker for molecular diagnostics of spinetoram resistance.
Ski Resort Closures and Opportunities for Sustainability in North America
Authors: Moscovici Daniel
Published: March 2022

More than half of the ski resorts in North America have closed since the early building booms—many facing a warming climate and pressures to find water to make artificial snow. Researching and documenting all resorts between 1969–2019, we find that 59% of all resorts in North America have closed since the resort boom of the 1960s and 70s (65% in the United States, 31% in Canada). This shift has left some states or provinces with only one or no resorts remaining. To proactively persevere with a variable climate, less water, and a need for more energy to make snow, we suggest mountains holistically plan for sustainability. Recommendations include third party environmental certification, commitment to sustainability at the management level, communication to customers about sustainability practices and implementing unique models for remaining open and competitive. These practices include resort consolidation, multi-mountain passes, and/or unique ownership models. We believe that ski resorts must focus on positive environmental practices, sustainability planning, and climate change adaptation if they want to remain viable and competitive in the coming decades.
Call Her Beloved: A Lexicon for Abjection in Toni Morrison’s The Bluest Eye and Beloved
Authors: Kumar S. Satish
Published: March 2022

What does it mean to mourn for the loss of lives that are rendered ungrievable by history? More importantly, with what language does one grieve the loss or despoliation of lives that are rendered ungrievable through disremembrance? This study reads such concerns as represented in two novels by Toni Morrison: The Bluest Eye and Beloved. Drawing on theorizations of the Other and the Abject in the philosophies of Emmanuel Levinas and Julia Kristeva, respectively, the readings of Morrison’s novels presented here seek to conceptualize the impacts of racial and racist oppression as the fallout from experiences of othering in the extreme. Confronting the desecration of human life and dignity engendered through racism, the study argues, is a descent into abjection. Through exploring Morrison’s narrative project, as explained in her non-fiction, this study seeks to conceptualize a possible lexicon for grieving the Abject without appropriating it or in any way diminishing its specific and radical alterity as a despoiled being.
NiO–Ba0.95Ca0.05Ce0.9Y0.1O3−δ as a Modified Anode Material Fabricated by the Tape Casting Method
Authors: Dudek Magdalena , Lis Bartłomiej , Kluczowski Ryszard , Krauz Mariusz , Ziąbka Magdalena , Gajek Marcin , Rapacz-Kmita Alicja , Mosiałek Michał , Dudek Piotr , Majda Dorota , Raźniak Andrzej
Published: March 2022

The development of new chemically resistant anodes for protonic ceramic fuel cells (PCFCs) is urgently required to avoid the costly deep hydrogen purification method. Ba0.95Ca0.05Ce0.9Y0.1O3−δ (5CBCY), which is more chemically resistant than BaCaCe0.9Y0.1O3−δ, was here tested as a component of a composite NiO–5CBCY anode material. A preparation slurry comprising 5CBCY, NiO, graphite, and an organic medium was tape cast, sintered and subjected to thermal treatment in 10 vol.% H2 in Ar at 700 °C. Differential thermal analysis, thermogravimetry, quadrupole mass spectrometry, X-ray diffraction analysis, scanning electron microscopy, the AC four-probe method and electrochemical impedance spectroscopy were used for the investigation. The electrical conductivity of the Ni–5CBCY in H2–Ar at 700 °C was 1.1 S/cm. In the same gas atmosphere but with an additional 5 vol.% CO2, it was slightly lower, at 0.8 S/cm. The Ni–5CBCY cermet exhibited repeatable electrical conductivity values during Ni-to-NiO oxidation cycles and NiO-to-Ni reduction in the 5CBCY matrix, making it sufficient for preliminary testing in PCFCs.
A Mixing Model for Describing Electrical Conductivity of a Woven Structure
Authors: Tokarska Magdalena
Published: March 2022

The main aim of the research was to describe electro-conductive woven structures by specifying the phases’ exponents using the generalised Archie’s law. Special woven structures were designed to transfer Archie’s model to the textile object. The woven structure was treated as a complex multiphase mixture. The structure was composed of two conducting phases (strips and strip contacts) and one non-conducting phase (pore space). It was found that the designed structures were characterised by the phases’ exponents that exceeded the value of 2, which denoted low connectivity in the conductive phases. A qualitative and quantitative description of the woven structure was feasible, i.e., the connectedness and the connectivity, respectively. The connectedness of both of the phases was dependent on the material from which the structure was designed. The fraction of each of the phases involved in the current conductivity was important. The connectivity connected with structure density, in varying degrees, affected the electro-conductive properties of the woven structure. It was important how the phases were arranged in the whole composite. It was found that the strips’ contact phases played an important role in the structure of the composite.
Parametric Optimization of Thin-Walled 3D Beams with Perforation Based on Homogenization and Soft Computing
Authors: Gajewski Tomasz , Staszak Natalia , Garbowski Tomasz
Published: March 2022

The production of thin-walled beams with various cross-sections is increasingly automated and digitized. This allows producing complicated cross-section shapes with a very high precision. Thus, a new opportunity has appeared to optimize these types of products. The optimized parameters are not only the lengths of the individual sections of the cross section, but also the bending angles and openings along the beam length. The simultaneous maximization of the compressive, bending and shear stiffness as well as the minimization of the production cost or the weight of the element makes the problem a multi-criteria issue. The paper proposes a complete procedure for optimizing various open sections of thin-walled beam with different openings along its length. The procedure is based on the developed algorithms for traditional and soft computing optimization as well as the original numerical homogenization method. Although the work uses the finite element method (FEM), no computational stress analyses are required, i.e., solving the system of equations, except for building a full stiffness matrix of the optimized element. The shell-to-beam homogenization procedure used is based on equivalence strain energy between the full 3D representative volume element (RVE) and its beam representation. The proposed procedure allows for quick optimization of any open sections of thin-walled beams in a few simple steps. The procedure can be easily implemented in any development environment, for instance in MATLAB, as it was done in this paper.
Mathematical Modeling and Analytical Solution of Thermoelastic Stability Problem of Functionally Graded Nanocomposite Cylinders within Different Theories
Authors: Avey Mahmure , Fantuzzi Nicholas , Sofiyev Abdullah
Published: March 2022

Revolutionary advances in technology have led to the use of functionally graded nanocomposite structural elements that operate at high temperatures and whose properties depend on position, such as cylindrical shells designed as load-bearing elements. These advances in technology require new mathematical modeling and updated numerical calculations to be performed using improved theories at design time to reliably apply such elements. The main goal of this study is to model, mathematically and within an analytical solution, the thermoelastic stability problem of composite cylinders reinforced by carbon nanotubes (CNTs) under a uniform thermal loading within the shear deformation theory (ST). The influence of transverse shear deformations is considered when forming the fundamental relations of CNT-patterned cylindrical shells and the basic partial differential equations (PDEs) are derived within the modified Donnell-type shell theory. The PDEs are solved by the Galerkin method, and the formula is found for the eigenvalue (critical temperature) of the functionally graded nanocomposite cylindrical shells. The influences of CNT patterns, volume fraction, and geometric parameters on the critical temperature within the ST are estimated by comparing the results within classical theory (CT).
Certain New Models of the Multi-Space Fractal-Fractional Kuramoto-Sivashinsky and Korteweg-de Vries Equations
Authors: Srivastava Hari M. , Saad Khaled Mohammed , Hamanah Walid M.
Published: March 2022

The main objective of this paper is to introduce and study the numerical solutions of the multi-space fractal-fractional Kuramoto-Sivashinsky equation (MSFFKS) and the multi-space fractal-fractional Korteweg-de Vries equation (MSFFKDV). These models are obtained by replacing the classical derivative by the fractal-fractional derivative based upon the generalized Mittag-Leffler kernel. In our investigation, we use the spectral collocation method (SCM) involving the shifted Legendre polynomials (SLPs) in order to reduce the new models to a system of algebraic equations. We then use one of the known numerical methods, the Newton-Raphson method (NRM), for solving the resulting system of the nonlinear algebraic equations. The efficiency and accuracy of the numerical results are validated by calculating the absolute error as well as the residual error. We also present several illustrative examples and graphical representations for the various results which we have derived in this paper.
Lightweight Image Super-Resolution Based on Local Interaction of Multi-Scale Features and Global Fusion
Authors: Meng Zhiqing , Zhang Jing , Li Xiangjun , Zhang Lingyin
Published: March 2022

In recent years, computer vision technology has been widely applied in various fields, making super-resolution (SR), a low-level visual task, a research hotspot. Although deep convolutional neural network has made good progress in the field of single-image super-resolution (SISR), its adaptability to real-time interactive devices that require fast response is poor due to the excessive amount of network model parameters, the long inference image time, and the complex training model. To solve this problem, we propose a lightweight image reconstruction network (MSFN) for multi-scale feature local interaction based on global connection of the local feature channel. Then, we develop a multi-scale feature interaction block (FIB) in MSFN to fully extract spatial information of different regions of the original image by using convolution layers of different scales. On this basis, we use the channel stripping operation to compress the model, and reduce the number of model parameters as much as possible on the premise of ensuring the reconstructed image quality. Finally, we test the proposed MSFN model with the benchmark datasets. The experimental results show that the MSFN model is better than the other state-of-the-art SR methods in reconstruction effect, computational complexity, and inference time.
An Intelligent Chimp Optimizer for Scheduling of IoT Application Tasks in Fog Computing
Authors: Attiya Ibrahim , Abualigah Laith , Elsadek Doaa , Chelloug Samia Allaoua , Abd Elaziz Mohamed
Published: March 2022

The cloud computing paradigm is evolving rapidly to address the challenges of new emerging paradigms, such as the Internet of Things (IoT) and fog computing. As a result, cloud services usage is increasing dramatically with the recent growth of IoT-based applications. To successfully fulfill application requirements while efficiently harnessing cloud computing power, intelligent scheduling approaches are required to optimize the scheduling of IoT application tasks on computing resources. In this paper, the chimp optimization algorithm (ChOA) is incorporated with the marine predators algorithm (MPA) and disruption operator to determine the optimal solution to IoT applications’ task scheduling. The developed algorithm, called CHMPAD, aims to avoid entrapment in the local optima and improve the exploitation capability of the basic ChOA as its main drawbacks. Experiments are conducted using synthetic and real workloads collected from the Parallel Workload Archive to demonstrate the applicability and efficiency of the presented CHMPAD method. The simulation findings reveal that CHMPAD can achieve average makespan time improvements of 1.12–43.20% (for synthetic workloads), 1.00–43.43% (for NASA iPSC workloads), and 2.75–42.53% (for HPC2N workloads) over peer scheduling algorithms. Further, our evaluation results suggest that our proposal can improve the throughput performance of fog computing.
Novel PKD2 Missense Mutation p.Ile424Ser in an Individual with Multiple Hepatic Cysts: A Case Report
Authors: Miura Seiko , Niida Yo , Hashizume Chieko , Fujii Ai , Takagaki Yuta , Kusama Kahoru , Akazawa Sumiyo , Minami Tetsuya , Mukai Tsuyoshi , Furuichi Kengo , Tsuchishima Mutsumi , Ueda Nobuhiko , Takamura Hiroyuki , Koya Daisuke , Ito Tohru
Published: March 2022

We report a novel missense mutation, p.Ile424Ser, in the PKD2 gene of an autosomal dominant polycystic kidney disease (ADPKD) patient with multiple liver cysts. A 57-year-old woman presented to our university hospital with abdominal fullness, decreasing appetite, and dyspnea for three months. A percutaneous drainage of hepatic cysts was performed with no significant symptomatic relief. A computed tomography (CT) scan revealed a hepatic cyst in the lateral portion of the liver with appreciable compression of the stomach. Prior to this admission, the patient had undergone three drainage procedures with serial CT-based follow-up of the cysts over the past 37 years. With a presumptive diagnosis of extrarenal manifestation of ADPKD, we performed both a hepatic cystectomy and a hepatectomy. Because the patient reported a family history of hepatic cysts, we conducted a postoperative genetic analysis. A novel missense mutation, p.Ile424Ser, was detected in the PKD2 gene. Mutations in either the PKD1 or PKD2 genes account for most cases of ADPKD. To the extent of our knowledge, this point mutation has not been reported in the general population. Our in-silico analysis suggests a hereditary likely pathogenic mutation.
Superconductivity 2022
Authors: Koblischka Michael Rudolf , Koblischka-Veneva Anjela
Published: March 2022

Superconductivity in metals and alloys, i.e., conventional superconductivity, has seen many new developments in recent years, leading to a renewed interest in the principles of superconductivity and the search for new materials. The most striking discoveries include the near-room-temperature superconductivity in metal hydrides (LaH10) under pressure, the extreme stability of superconductivity in NbTi up to 261 GPa pressure, the discovery of high-entropy alloy (HEA) superconductor materials, and the machine learning prediction of new superconducting materials. Other interesting research concerns the properties of 2D superconductors, topological superconductors, e.g., in hybrid systems, and the use of nanotechnology to create nanowires and nanostructures with new properties. Furthermore, and most importantly, the drive from new accelerator and fusion reactors for stronger superconducting magnets has lead to improved cable materials, showing the highest critical current densities ever. Thus, this Special Issue aims to bring together a collection of papers reflecting the present activity in this field.
Electron Backscatter Diffraction (EBSD) Analysis of Machinable Lead-Free Brass Alloys: Connecting Texture with Fracture
Authors: Vazdirvanidis Athanasios , Rikos Andreas , Toulfatzis Anagnostis I. , Pantazopoulos George A.
Published: March 2022

The current paper is related to the study of the microstructure and texture of two machinable lead-free brass alloys, namely CuZn42 (CW510L) and CuZn38As (CW511L), which were evaluated in the as-drawn and post heat treated condition. Electron backscatter diffraction (EBSD) was employed for the examination of the brass rods’ crystallographic properties in order to correlate the effect of post processing heat treatment on the evolution of phase structure and texture towards the interpretation of dynamic (impact) fracture properties. It is shown that α- and β-phase volume fractions, mean grain size, and grain boundary misorientation are the most influential factors altering the fracture resistance of single- and dual-phase brass alloy rods. The role of grain boundary engineering, through the formation of coincidence site lattice (CSL) boundaries and their evolution during thermomechanical processing, is of major importance for the design of the mechanical behaviour of new eco-friendly machinable brass alloys.
The Contribution of Various Plasticity Mechanisms to the Deformation Behavior of Gradient Nanograined FeNi Alloy
Authors: Korchuganov Aleksandr V. , Zolnikov Konstantin P. , Kryzhevich Dmitrij S.
Published: March 2022

This paper investigates the deformation behavior of a gradient grained FeNi sample under uniaxial tension using molecular dynamics simulations. The simulated sample consists of five layers with grains of the same size in each layer ranging from 10 to 30 nm. It is shown that the sample plasticity develops through sequential activation of different mechanisms. These are either the generation of certain structural defects, or grain boundary migration, or grain boundary sliding. The onset of plasticity is provided by partial dislocations that produce stacking faults in large grains. Other mechanisms involved in plastic deformation are the nucleation of trailing/full dislocations and twinning, which gradually affect smaller and smaller grains. Grain boundary sliding is more intensive in smallest grains due to their less constraint. Grain boundary migration generally leads to the growth of large grains. At strains below 7.0%, plasticity is mainly contributed by the evolution of stacking faults. At higher strains, the main plasticity mechanisms are twinning and grain boundary migration. As the strain increases, the maximum values of accumulated shear, the density of intragranular defects, and the number of atoms involved in intergranular rearrangements are observed first in large, then in medium, and finally in small grains.
Downregulation of Squalene Synthase Broadly Impacts Isoprenoid Biosynthesis in Guayule
Authors: Placido Dante , Dong Niu , Amer Bashar , Dong Chen , Ponciano Grisel , Kahlon Talwinder , Whalen Maureen , Baidoo Edward E. K. , McMahan Colleen
Published: March 2022

Production of natural rubber by Parthenium argentaum (guayule) requires increased yield for economic sustainability. An RNAi gene silencing strategy was used to engineer isoprenoid biosynthesis by downregulation of squalene synthase (SQS), such that the pool of farnesyl diphosphate (FPP) substrate might instead be available to initiate natural rubber synthesis. Downregulation of SQS resulted in significantly reduced squalene and slightly increased rubber, but not in the same tissues nor to the same extent, partially due to an apparent negative feedback regulatory mechanism that downregulated mevalonate pathway isoprenoid production, presumably associated with excess geranyl pyrophosphate levels. A detailed metabolomics analysis of isoprenoid production in guayule revealed significant differences in metabolism in different tissues, including in active mevalonate and methylerythritol phosphate pathways in stem tissue, where rubber and squalene accumulate. New insights and strategies for engineering isoprenoid production in guayule were identified.
Evaluation of the Links between Lamb Feed Efficiency and Rumen and Plasma Metabolomic Data
Authors: Touitou Florian , Tortereau Flavie , Bret Lydie , Marty-Gasset Nathalie , Marcon Didier , Meynadier Annabelle
Published: March 2022

Feed efficiency is one of the keystones that could help make animal production less costly and more environmentally friendly. Residual feed intake (RFI) is a widely used criterion to measure feed efficiency by regressing intake on the main energy sinks. We investigated rumen and plasma metabolomic data on Romane male lambs that had been genetically selected for either feed efficiency (line rfi−) or inefficiency (line rfi+). These investigations were conducted both during the growth phase under a 100% concentrate diet and later on under a mixed diet to identify differential metabolite expression and to link it to biological phenomena that could explain differences in feed efficiency. Nuclear magnetic resonance (NMR) data were analyzed using partial least squares discriminant analysis (PLS-DA), and correlations between metabolites’ relative concentrations were estimated to identify relationships between them. High levels of plasma citrate and malate were associated with genetically efficient animals, while high levels of amino acids such as L-threonine, L-serine, and L-leucine as well as beta-hydroxyisovalerate were associated with genetically inefficient animals under both diets. The two divergent lines could not be discriminated using rumen metabolites. Based on phenotypic residual feed intake (RFI), efficient and inefficient animals were discriminated using plasma metabolites determined under a 100% concentrate diet, but no discrimination was observed with plasma metabolites under a mixed diet or with rumen metabolites regardless of diet. Plasma amino acids, citrate, and malate were the most discriminant metabolites, suggesting that protein turnover and the mitochondrial production of energy could be the main phenomena that differ between efficient and inefficient Romane lambs.
Effect of Wetting Characteristics of Polishing Fluid on the Quality of Water-Dissolution Polishing of KDP Crystals
Authors: Wang Xu , Gao Hang , Deng Qianfa , Wang Jinhu , Chen Hongyu , Yuan Julong
Published: March 2022

KDP crystals constitute the only laser-frequency conversion and electro-optical switches that can be used in laser systems for inertial confinement fusion. However, KDP crystals are difficult to produce because of their inherent softness, brittleness, water-solubility, and temperature sensitivity. The authors’ group developed a water-dissolution polishing method in previous studies to obtain near-damage-free KDP surfaces. In this article, the effect of the wetting characteristics of the water dissolution polishing fluid on the crystal surface—a factor rarely considered in the usual process optimization—on the polished surface quality was comprehensively studied. The mean radius of micro water droplets at 5 wt.% and 7.5 wt.% water content was approximately 0.6 nm and 1.2 nm, respectively. Theoretically, the smaller micro water droplet size is beneficial to the polished surface quality. When the water content was 5 wt.%, due to the poor wetting characteristics of the polishing fluid, surface scratches appeared on the polished surface; when the water content was 7.5 wt.%, the effects of the wetting characteristics and the radius of the micro water droplets reached a balance, and the polished surface quality was the best (Ra 1.260 nm). These results confirm that the wetting characteristics of the polishing fluid constitute one of the key factors that must be considered. This study proves that the wetting characteristics of the polishing fluid should be improved during the optimization process of polishing fluid composition when using oil-based polishing fluids for ultra-precision polishing.
Metabolism of the Genus Guyparkeria Revealed by Pangenome Analysis
Authors: Lau Vetter Maggie C. Y. , Huang Baowei , Fenske Linda , Blom Jochen
Published: March 2022

Halophilic sulfur-oxidizing bacteria belonging to the genus Guyparkeria occur at both marine and terrestrial habitats. Common physiological characteristics displayed by Guyparkeria isolates have not yet been linked to the metabolic potential encoded in their genetic inventory. To provide a genetic basis for understanding the metabolism of Guyparkeria, nine genomes were compared to reveal the metabolic capabilities and adaptations. A detailed account is given on Guyparkeria’s ability to assimilate carbon by fixation, to oxidize reduced sulfur, to oxidize thiocyanate, and to cope with salinity stress.
Fracture Evolution between Blasting Roof Cutting Holes in a Mining Stress Environment
Authors: Tu Min , Zhao Gaoming , Zhang Xiangyang , Bu Qingwei , Dang Jiaxin
Published: March 2022

The problem of strong ground pressure caused by thick and hard roofs in coal mine stopes has become more prominent. Blasting roof cutting and pressure relief are effective technical ways to solve this engineering problem. The key to achieving roof cutting and pressure relief is the evolution and penetration of cracks between blasting roof cutting holes in a mining stress environment. To solve this problem, research on the evolution of cracks between blasting roof cutting holes in a mining stress environment is being conducted. Along with specific engineering examples, the mechanical effect of blasting dynamic load in the surrounding rock medium between holes in a mining stress environment is analyzed using the research methods of field investigation, blasting simulation, and mechanical analysis. The fracture evolution law between blasting roof cutting holes under the conditions of different hole diameters and spacings, confining pressure environments, and rock mass strengths is analyzed using the univariate comparative analysis method. The research shows that the superposition effect of blasting dynamic load between adjacent blastholes in the transmission process leads to the “X” type penetration evolution of surrounding rock fracture area and fracture area between blastholes. Among them, the blasting effect of a hole diameter of 70 mm and a hole spacing of 0.8 m is best. When designing the blasting roof cutting scheme, we should pay attention to the key factors such as hole diameter and hole spacing. The hole spacing is particularly critical to the effect of roof cutting and crack formation, whereas the confining pressure and rock mass strength have little impact on the expansion and development of blasting cracks, which can be listed as secondary factors for comprehensive analysis in design. According to the transmission law of blasting dynamic load between holes, the critical criterion of blasting top clearance penetration under mining stress environment is deduced. Four important factors affecting blasting top cutting effect are simulated and studied for optimizing the design of roof cutting scheme. Practical engineering application results show that the blasting roof cutting scheme achieves a good seam- forming effect and creates good initial conditions for thick and hard roof cutting. It can serve as a reference for the decision making of blasting top caving technology under similar engineering conditions.
Computational Methods for Understanding the Selectivity and Signal Transduction Mechanism of Aminomethyl Tetrahydronaphthalene to Opioid Receptors
Authors: Xie Peng , Zhang Junjie , Chen Baiyu , Li Xinwei , Zhang Wenbo , Zhu Mengdan , Li Wei , Li Jianqi , Fu Wei
Published: March 2022

Opioid receptors are members of the group of G protein-couple receptors, which have been proven to be effective targets for treating severe pain. The interactions between the opioid receptors and corresponding ligands and the receptor’s activation by different agonists have been among the most important fields in opioid research. In this study, with compound M1, an active metabolite of tramadol, as the clue compound, several aminomethyl tetrahydronaphthalenes were designed, synthesized and assayed upon opioid receptors. With the resultant compounds FW-AII-OH-1 (Ki = 141.2 nM for the κ opioid receptor), FW-AII-OH-2 (Ki = 4.64 nM for the δ opioid receptor), FW-DI-OH-2 (Ki = 8.65 nM for the δ opioid receptor) and FW-DIII-OH-2 (Ki = 228.45 nM for the δ opioid receptor) as probe molecules, the structural determinants responsible for the subtype selectivity and activation mechanisms were further investigated by molecular modeling and molecular dynamics simulations. It was shown that Y7.43 was a key residue in determining the selectivity of the three opioid receptors, and W6.58 was essential for the selectivity of the δ opioid receptor. A detailed stepwise discovered agonist-induced signal transduction mechanism of three opioid receptors by aminomethyl tetrahydronaphthalene compounds was proposed: the 3–7 lock between TM3 and TM7, the DRG lock between TM3 and TM6 and rearrangement of I3.40, P5.50 and F6.44, which resulted in the cooperative movement in 7 TMs. Then, the structural relaxation left room for the binding of the G protein at the intracellular site, and finally the opioid receptors were activated.
Salivary MMP-9 Levels in Chronic Periodontitis Patients with Type-II Diabetes Mellitus
Authors: Arshad Romaisa , Ismail Waleed A. , Zara Batool , Naseer Rabia , Minhas Sadia , Ansari Moeez , Akhter Fatema , Khokhar Saria A. , Alqahtani Abdullah Ali , Abutayyem Huda , Alswairki Haytham Jamil , Alam Mohammad Khursheed
Published: March 2022

Chronic periodontitis and diabetes mellitus share a two-way relationship, the common factor being the inflammatory-mediated pathway, and various cytokines are released during this inflammatory cascade, one of which being matrix metalloproteinase-9. The aim of this study was to identify whether the levels of matrix metalloproteinase-9 are increased due to type-II diabetes mellitus in chronic periodontitis patients. It was an observational, analytical, case-control study. Thirty subjects were recruited in the test group, who were suffering from type-II diabetes mellitus and chronic periodontitis, and 30 subjects in the control group, who were subjects with chronic periodontitis but systemically healthy. Periodontal parameters, including the plaque score, gingival bleeding index, probing pocket depth and clinical attachment level of the subjects, were measured, saliva samples of all of the subjects were collected and salivary matrix metalloproteinase-9 levels were analyzed by an enzyme-linked immunosorbent assay (ELISA) technique. The statistical analysis was performed using SPSS 24. Overall, the matrix metalloproteinase-9 levels of the diabetic patients with chronic periodontitis were increased almost twofold (156.95 ± 29.80 ng/mL) compared to the levels in the controls (74.96 ± 6.32 ng/mL) (p < 0.001). Similarly, the periodontal parameters were far worse in diabetics with chronic periodontitis compared to the controls. The average gingivitis score for the test subjects was 78.45 ± 8.93%), compared to 29.32 ± 12.96% in the controls (p < 0.001). The mean probing pocket depth for the test group was 5.39 ± 0.60 mm, and, for the control group, it was 4.35 ± 0.31 mm (p < 0.001). For the test subjects, the average clinical attachment level was 5.86 ± 0.58 mm, and it was 4.66 ± 0.32 mm for the controls (p < 0.001). It was ascertained that there is a two-fold increase in the levels of salivary matrix metalloproteinase-9 in the test group compared to the control group. In addition, the level of periodontal apparatus destruction was greater in the test group. This proved that type-II diabetes mellitus influences the levels of matrix metalloproteinase-9 in humans and elevates them, causing further periodontal destruction.