bluuebunny/crossref_metadata_2025 huggingface dataset:
Therapeutic Drugs and Devices for Tackling Ocular Hypertension and Glaucoma, and Need for Neuroprotection and Cytoprotective Therapies
Authors: Sharif Najam A.
Published: September 2021

Damage to the optic nerve and the death of associated retinal ganglion cells (RGCs) by elevated intraocular pressure (IOP), also known as glaucoma, is responsible for visual impairment and blindness in millions of people worldwide. The ocular hypertension (OHT) and the deleterious mechanical forces it exerts at the back of the eye, at the level of the optic nerve head/optic disc and lamina cribosa, is the only modifiable risk factor associated with glaucoma that can be treated. The elevated IOP occurs due to the inability of accumulated aqueous humor (AQH) to egress from the anterior chamber of the eye due to occlusion of the major outflow pathway, the trabecular meshwork (TM) and Schlemm’s canal (SC). Several different classes of pharmaceutical agents, surgical techniques and implantable devices have been developed to lower and control IOP. First-line drugs to promote AQH outflow via the uveoscleral outflow pathway include FP-receptor prostaglandin (PG) agonists (e.g., latanoprost, travoprost and tafluprost) and a novel non-PG EP2-receptor agonist (omidenepag isopropyl, Eybelis®). TM/SC outflow enhancing drugs are also effective ocular hypotensive agents (e.g., rho kinase inhibitors like ripasudil and netarsudil; and latanoprostene bunod, a conjugate of a nitric oxide donor and latanoprost). One of the most effective anterior chamber AQH microshunt devices is the Preserflo® microshunt which can lower IOP down to 10–13 mmHg. Other IOP-lowering drugs and devices on the horizon will be also discussed. Additionally, since elevated IOP is only one of many risk factors for development of glaucomatous optic neuropathy, a treatise of the role of inflammatory neurodegeneration of the optic nerve and retinal ganglion cells and appropriate neuroprotective strategies to mitigate this disease will also be reviewed and discussed.
Emerging Applications of Metabolomics to Assess the Efficacy of Traditional Chinese Medicines for Treating Type 2 Diabetes Mellitus
Authors: Zhang Yumeng , Yang Yingbo , Ding Lili , Wang Zhengtao , Xiao Ying , Xiao Wei
Published: September 2021

Diabetes is a common and complex disease that can exacerbate the complications related to cardiovascular disease, and this is especially true for type 2 diabetes mellitus (T2DM). In addition to the standard pharmacological therapies, T2DM has also been treated with nonconventional regimens such as traditional Chinese medicine (TCM), e.g., herbal medicines and TCM prescriptions, although the mechanisms underlying the therapeutic benefits remain unclear. In this regard, many studies have used metabolomics technology to elucidate the basis for the efficacy of TCM for T2DM. Metabolomics has recently attracted much attention with regard to drug discovery and pharmacologically relevant natural products. In this review, we summarize the application of metabolomics to the assessment of TCM efficacy for treating T2DM. Increasing evidence suggests that the metabolic profile of an individual patient may reflect a specific type of T2DM syndrome, which may provide a new perspective for disease diagnosis. In addition, TCM has proved effective for countering the metabolic disorders related to T2DM, and this may constitute the basis for TCM efficacy. Therefore, further determining how TCM contributes to the reversal of metabolic disorders, such as using network pharmacology or by assessing the contribution of host–gut microbiota interactions, will also provide researchers with new potential targets for pharmacologic-based therapies.
Pithecellobium clypearia: Amelioration Effect on Imiquimod-Induced Psoriasis in Mice Based on a Tissue Metabonomic Analysis
Authors: Li Ying , Zong Jiaxin , Ye Wenjun , Fu Yuanfeng , Gu Xinyi , Pan Weisong , Yang Li , Zhang Ting , Zhou Mingmei
Published: September 2021

Pithecellobium clypearia Benth. (accepted name: Archidendron clypearia (Jack) I.C.Nielsen; Mimosaceae), a popular traditional Chinese medicine, has a significant anti-inflammatory effect. The crude water extract of the aerial part of P. clypearia has been clinically applied to treat upper respiratory tract infections, acute gastroenteritis, laryngitis, and pharyngitis. However, the therapeutic mechanism of ethanol fraction of water extract (ESW) of P. clypearia to treat psoriasis should be complemented. The aim of our research was to clarify the protective effects of ESW from P. clypearia against psoriasis-like skin inflammation induced by imiquimod (IMQ) in mice with efficacy indexes and target tissue (spleen and serum) metabolomics. The ingredient of ESW was analyzed by ultrahigh-performance liquid chromatography combined with tandem mass spectrometry (UHPLC-MS/MS) method. The imiquimod-induced psoriatic mouse model was employed to investigate the effect of ESW against psoriasis, where the treatment method was implemented for 6 days both topically (Gel at 5%) and orally (at 2.4 g/kg p.o.). Traditional pharmacodynamic indicators (phenotypic characteristics, psoriasis area and severity index (PASI) score, H&E staining, immunohistochemical staining, the thickness of epidermis, body weight change, and spleen index) were conducted to appraise the efficacy of ESW. Furthermore, a gas chromatography-mass spectrometer (GC-MS) coupled with multivariate analysis was integrated and applied to obtain serum and spleen metabolic profiles for clarifying metabolic regulatory mechanisms of ESW. The current study illustrated that ESW is composed mainly of gallic acid, ethyl gallate, quercitin, 7-O-galloyltricetiflavan, quercetin, and myricetin by UHPLC-MS/MS analysis. ESW could distinctly improve IMQ-induced psoriasis in mouse through reducing PASI score, alleviating tissue damage, restoring spleen index, and inhibiting proliferating cell nuclear antigen (PCNA) expression in psoriasis-like skin tissue. From the metabolomics study, 23 markers with significant changes are involved in eight main pathways in spleen and serum samples, including linoleic acid metabolism and glycine, serine, and threonine metabolism. The current study showed that ESW had obvious antipsoriasis effects on IMQ-induced psoriasis in mice, which might be attributed to regulating the dysfunction of differential biomarkers and related pathways. In summary, ESW of P. clypearia showed a favourable therapeutic effect on IMQ-induced psoriasis, and metabolomics provided insights into the mechanisms of ESW to the treatment of psoriasis.
Equivalence of Dissipative and Dissipationless Dynamics of Interacting Quantum Systems With Its Application to the Unitary Fermi Gas
Authors: Tokieda Masaaki , Endo Shimpei
Published: September 2021

We analytically study quantum dissipative dynamics described by the Caldirola-Kanai model with inter-particle interactions. We have found that the dissipative quantum dynamics of the Caldirola-Kanai model can be exactly mapped to a dissipationless quantum dynamics under a negative external harmonic potential, even when the particles are strongly interacting. In particular, we show that the mapping is valid for the unitary Fermi gas, which is relevant for cold atoms and nuclear matters.
Effect of Cold Stress on Neurobehavioral and Physiological Parameters in Rats
Authors: El Marzouki Hajar , Aboussaleh Youssef , Najimi Mohamed , Chigr Fatiha , Ahami Ahmed
Published: September 2021

Objective: Cold stress is an important current issue and implementing control strategies to limit its sometimes harmful effects is crucial. Cold is a common stressor that can occur in our work and our occupational or leisure time activities every day. There are substantial studies on the effects of chronic stress on memory and behavior, although, the cognitive changes and anxiety disorders that can occur after exposure to chronic intermittent cold stress are not completely characterized. Therefore, the present study was undertaken with an aim to investigate the effects of chronic intermittent cold stress on body weight, food intake and working memory, and to elucidate cold stress related anxiety disorders using cognitive and behavioral test batteries.

Methods: We generated a cold stress model by exposing rats to chronic intermittent cold stress for 5 consecutive days and in order to test for the potential presence of sex differences, a comparable number of male and female rats were tested in the current study. Then, we measured the body weights, food intake and the adrenal glands weight. Working memory and recognition memory were assessed using the Y maze and the Novel Object Recognition (NOR) tasks. While, sex differences in the effects of chronic stress on behavior were evaluated by the elevated plus maze (EPM), open field maze (OF), and Marble burying (MB) tests.

Results: We found that 2 h exposure to cold (4°C) resulted in an increase in the relative weight of the adrenal glands in male rats. Given the same chronic stress 5 days of cold exposure (2 h per day), increased weight gain in male rats, while females showed decreased food intake and no change in body weight. Both sexes successfully performed the Y maze and object recognition (OR) tasks, indicating intact spatial working memory performance and object recognition abilities in both male and female rats. In addition, we have shown that stress caused an increase in the level of anxiety in male rats. In contrast, the behavior of the female rats was not affected by cold exposure.

Conclusion: Overall, the current results provide preliminary evidence that chronic intermittent cold stress model may not be an efficient stressor to female rats. Females exhibit resilience to cold exposure that causes an increase in the level of anxiety in male rats, which demonstrates that they are affected differently by stress and the gender is an important consideration in experimental design.
Synthesis and Evaluation of New Halogenated GR24 Analogs as Germination Promotors for Orobanche cumana
Authors: Chen Yuchao , Kuang Yi , Shi Liyang , Wang Xing , Fu Haoyu , Yang Shengxiang , Sampietro Diego A. , Huang Luqi , Yuan Yuan
Published: September 2021

Orobanche and Striga are parasitic weeds extremely well adapted to the life cycle of their host plants. They cannot be eliminated by conventional weed control methods. Suicidal germination induced by strigolactones (SLs) analogs is an option to control these weeds. Here, we reported two new halogenated (+)-GR24 analogs, named 7-bromo-GR24 (7BrGR24) and 7-fluoro-GR24 (7FGR24), which were synthesized using commercially available materials following simple steps. Both compounds strongly promoted seed germination of Orobanche cumana. Their EC50 values of 2.3±0.28×10−8M (7BrGR24) and 0.97±0.29×10−8M (7FGR24) were 3- and 5-fold lower, respectively, than those of (+)-GR24 and rac-GR24 (EC50=5.1±1.32–5.3±1.44×10−8; p<0.05). The 7FGR24 was the strongest seed germination promoter tested, with a stimulation percentage of 62.0±9.1% at 1.0×10−8M and 90.9±3.8% at 1.0×10−6M. It showed higher binding affinity (IC50=0.189±0.012μM) for the SL receptor ShHTL7 than (+)-GR24 (IC50=0.248±0.032μM), rac-GR24 (IC50=0.319±0.032μM), and 7BrGR24 (IC50=0.521±0.087μM). Molecular docking experiments indicated that the binding affinity of both halogenated analogs to the strigolactone receptor OsD14 was similar to that of (+)-GR24. Our results indicate that 7FGR24 is a promising agent for the control of parasitic weeds.
Fine Mapping of a New Major QTL-qGLS8 for Gray Leaf Spot Resistance in Maize
Authors: Qiu Hongbo , Li Chunhong , Yang Wenzhu , Tan Kang , Yi Qiang , Yang Mei , Bai Guangxiao
Published: September 2021

Gray leaf spot (GLS), caused by different species of Cercospora, is a fungal, non-soil-borne disease that causes serious reductions in maize yield worldwide. The identification of major quantitative trait loci (QTLs) for GLS resistance in maize is essential for developing marker-assisted selection strategies in maize breeding. Previous research found a significant difference (P < 0.01) in GLS resistance between T32 (highly resistant) and J51 (highly susceptible) genotypes of maize. Initial QTL analysis was conducted in an F2 : 3 population of 189 individuals utilizing genetic maps that were constructed using 181 simple sequence repeat (SSR) markers. One QTL (qGLS8) was detected, defined by the markers umc1130 and umc2354 in three environments. The qGLS8 QTL detected in the initial analysis was located in a 51.96-Mb genomic region of chromosome 8 and explained 7.89–14.71% of the phenotypic variation in GLS resistance in different environments. We also developed a near isogenic line (NIL) BC3F2 population with 1,468 individuals and a BC3F2-Micro population with 180 individuals for fine mapping. High-resolution genetic and physical maps were constructed using six newly developed SSRs. The QTL-qGLS8 was narrowed down to a 124-kb region flanked by the markers ym20 and ym51 and explained up to 17.46% of the phenotypic variation in GLS resistance. The QTL-qGLS8 contained seven candidate genes, such as an MYB-related transcription factor 24 and a C3H transcription factor 347), and long intergenic non-coding RNAs (lincRNAs). The present study aimed to provide a foundation for the identification of candidate genes for GLS resistance in maize.
The Effect of Squad Rotation on Physical Activity at the 2018 World Cup in Russia. Analysis the Most Exploited Players of the 4 Best Teams
Authors: Kołodziejczyk Michał , Chmura Paweł , Konefał Marek , Chmura Jan , Rokita Andrzej , Andrzejewski Marcin
Published: September 2021

The purpose of this study was to examine how the four best teams in the 2018 Football Men's World Cup rotate by squad and how this impact the physical activity of the teams in consecutive rounds. The study sample consisted of the 31 players of the 4 best teams, who played in every tournament match, except for the third game of the group stage. The analysis included 186 observations and was carried out on the most exploited players (MEP) excluding goalkeepers, who played at least 450 mins (5 full matches) in the tournament. The analysis was conducted using data collected by an advanced motion analysis system known as STATS®. The selected physical activity parameters analyzed included: total distance covered (m/min), distance covered at various intensity ranges (m/min), top speed (km/h), and number of sprints performed. It was found that all four teams in the third match of the group stage have performed the largest number of rotations with most exploited players and introduced the highest number of rested players (7.75 ± 2.06). A significant increase was observed between the second and fourth match in the 0–7 km/h distance covered (37.99 ± 3.19–39.23 ± 3.35 m/min) and the top speed (28.12 ± 2.22–29.21 ± 2.64 km/h)—p < 0.05. Furthermore, MEPs in the knockout stage, used pacing strategies by increasing the amount of low-intensity running to maintain high intensity during the game. From a practical point of view, this investigation shows that squad rotation can be a valuable support to a pacing strategy by players.
Police Officers’ Ability in Recognizing Relevant Mental Health Conditions
Authors: Wittmann Linus , Groen Gunter , Hampel Petra , Petersen Ronja , Jörns-Presentati Astrid
Published: September 2021

The recognition of certain mental health conditions is important as this requires police officers to communicate and behave in an adjusted manner with affected individuals. The objective of the present study was to test police officers’ knowledge about mental health symptoms as a component of their mental health literacy (MHL) and to examine if police officers’ perceived knowledge corresponds with their actual knowledge. A questionnaire was used to assess for MHL representing mental health conditions which occur frequently in police requests (schizophrenia, bipolar disorder, depression, post-traumatic stress disorders, and emotionally unstable personality disorder). Furthermore, the questionnaire assessed the frequency of police requests, the officers’ perceived knowledge regarding mental disorders and their sense of feeling sufficiently trained to deal with these kinds of requests. Eighty-two police officers participated in the study. Police officers’ actual knowledge about mental health conditions did not correspond with their perceived knowledge. Participants revealed a moderately high level of overall knowledge which differed with regard to symptoms of each of the five mental health conditions. The mental status of a paranoid schizophrenia was best identified by the police officers and the majority correctly allocated the symptoms. Post-traumatic stress disorders and manic episodes were only identified by a minority of police offers. Police training geared to prepare for requests involving individuals with mental disorders should expand this limited knowledge transfer and focus on a broader variety of mental health conditions that police officers frequently encounter in requests.
An Evaluation of Age-Group Latent Mean Differences in Maladaptive Identity in Adolescence
Authors: Sharp Carla , Vanwoerden Salome , Schmeck Klaus , Birkhölzer Marc , Goth Kirstin
Published: September 2021

Little is known about the differences between age groups in maladaptive personality function as denoted in Criterion A of the Alternative Model for Personality Disorder (AMPD) in the DSM-5, which is the entry criterion for diagnosing personality disorder in the upcoming ICD-11. The current study aimed to address this gap by evaluating latent mean age group differences in maladaptive identity, which is one aspect that has been identified as an important feature of maladaptive, general personality function as represented in the DSM-5 and ICD-11. We were also interested whether mean differences would track with mean differences in borderline personality disorder (BPD) features given prior data suggesting that general personality function overlap with the construct of BPD. A community sample ofN= 2,381 adolescents, representing a mix of different socio-economic and educational backgrounds, ages 12-18 (M= 14.92,SD= 1.94; 46% male) completed a measure of maladaptive identity. A subset (n= 1,165) completed a measure of borderline personality features. Latent variable modeling was used to evaluate latent mean differences across seven age bands. Results suggested a normative increase in maladaptive identity after age 12, which remained consistent until age 17 when it dropped back to levels observed in 12-year-olds. Maladaptive identity was significantly associated with mean-level increases in borderline personality features, with these constructs becoming more closely associated with increasing age.
Utilization of Intravenous Ribavirin Among Reproductive Age Adults in 2010–2017: A Population-Based Study in the Yinzhou District, Ningbo City of China
Authors: Li Hailong , Zhao Houyu , Lin Hongbo , Shen Peng , Liu Cuili , Zhan Siyan
Published: September 2021

Background: Intravenous (IV) ribavirin is not approved in US and European Union, but it is authorized in China. Significant teratogenic and embryocidal effects of ribavirin have been found in almost all animal studies, it is critical to investigate the prevalence and trends of the utilization of IV ribavirin among reproductive age population.

Objective: To evaluate the prevalence and trends of IV ribavirin use among reproductive-age population in 2010–2017.

Methods: The study design of our study is retrospective cross-sectional study based on healthcare database. We identified and extracted the data of residents aged 18–44 years by using Yinzhou healthcare information database at 21 January, 2018. A cohort of IV ribavirin users were identified through outpatient prescription records in 3 general hospitals and 24 community health centers from 2010 to 2017. We reported the number, proportion, and prevalence of the exposure to IV ribavirin stratified by sex, age, marital status, education level, occupation, hospital level, calendar year, diagnosis, and dosage. The overall trends of IV ribavirin use, and the trends in different levels of hospital and common diagnoses were further analyzed and described.

Result: During the study period, the prevalence of IV ribavirin use among reproductive-age adults was 6.02% (48,287/801,667). Relatively higher prevalence were found in adults aged 40–44 (8.04%, 95% CI: 7.90–8.17), unmarried patients (8.91%, 95% CI: 8.74–9.08), and who had more than 9 years of education (6.82%, 95% CI: 6.74–6.90). Compared to secondary and tertiary hospitals, IV ribavirin was more likely to be dispensed in primary hospitals (19.44%, 95% CI: 19.28–19.61). The most common diagnoses were acute upper respiratory infections (AURIs), accounting for 80% of the patients exposed to IV ribavirin. For patients with AURIs, the prevalence of IV ribavirin was nearly 30%. Overall, the prevalence of IV ribavirin use decreased from 1.72% in 2010 to 0.24% in 2017.

Conclusion: We found IV ribavirin was mainly used for AURIs which suggested that a large amount of IV ribavirin use was probably inappropriate. The prevalence was decreasing by 87% over the past 8 years, and we encourage clinicians and pharmacists to continually avoid inappropriate use of IV ribavirin.
Dipeptidyl Peptidase 4 Inhibitors Decrease the Risk of Hepatocellular Carcinoma in Patients With Chronic Hepatitis C Infection and Type 2 Diabetes Mellitus: A Nationwide Study in Taiwan
Authors: Hsu Wei-Hao , Sue Shu-Ping , Liang Hsiu-Ling , Tseng Chin-Wei , Lin Hsiu-Chu , Wen Wei-Lun , Lee Mei-Yueh
Published: September 2021

Introduction: Dipeptidyl peptidase 4 inhibitors (DPP-4 inhibitors) are incretin-based oral antidiabetic drugs. Previous studies have shown an association between increased plasma activity of DPP-4 and chronic hepatitis C virus (HCV) infection. Dipeptidyl peptidase 4 inhibitors may be associated with preventing the development of chronic HCV infection. The aim of this study was to investigate whether the use of DPP-4 inhibitors is associated with a decreased risk of hepatocellular carcinoma (HCC) in patients with diabetes mellitus (DM) and chronic HCV infection.

Methods: In this retrospective cohort study, we enrolled patients with type 2 diabetes and chronic HCV infection from the National Health Insurance Research Database (NHIRD) in Taiwan. The patients were divided into two groups (DPP-4 inhibitor cohort and non-DPP-4 inhibitor cohort) according to whether or not they received DPP-4 inhibitor treatment.

Results: Multivariate Cox proportional hazard regression analysis showed a significantly lower risk of HCC in the patients who took DPP-4 inhibitors compared to those who did not. Kaplan-Meier survival analysis demonstrated a significantly higher HCC-free rate in the DPP-4 inhibitor cohort than in the non-DPP-4 inhibitor cohort.

Conclusion: The use of DPP-4 inhibitors was associated with a lower risk of HCC in patients with type 2 DM and chronic HCV infection.
Time to Revisit Oil Palm-Livestock Integration in the Wake of United Nations Sustainable Development Goals (SDGs)
Authors: Azhar Badrul , Tohiran Kamil Azmi , Nobilly Frisco , Zulkifli Raja , Syakir Muhammad Izzuddin , Ishak Zulkifli , Razi Norhisham , Oon Aqilah , Shahdan Ahmad , Maxwell Thomas M. R.
Published: September 2021

To date, the idea of using livestock animals as biological tools to manage weeds, sequester carbon, and boost food security in oil palm plantations has not been seriously considered by industry stakeholders of major producing countries (e.g., Indonesia, Malaysia, Thailand, Colombia, and Nigeria). We revisit the integration of oil palm cultivation with livestock farming as a silvopastoral agroforestry practice in the wake of Sustainable Development Goals (SDGs). Oil palm-livestock integration has the potential to promote sustainable palm oil production because it can provide multiple environmental and socio-economic benefits, including carbon sequestration, restoring top soil, improving ecosystem biodiversity, reducing pesticide and fertilizer inputs, and boosting national food security. In contrast to monocultural outputs of most conventional plantations, an oil palm silvopastoral system is an ideal way to address the global food insecurity challenge as it produces bioenergy, vegetable oil/fat and animal-based protein sources (e.g., red meat). In addition, the potential of contract targeted grazing could be considered as a new type of business and income diversification for rural people. Oil palm-livestock integration is a strategy by the palm oil industry to achieve multiple SDGs. Out of the 17 SDGs, oil palm-livestock integration is likely to deliver nine SDGs. Palm oil certification bodies should recognize oil palm-livestock integration as a biological control method in weed management practices. We recommend that oil palm-livestock integration should be promoted to revitalize sustainable palm oil production and strategic biodiversity conservation policy. Policy makers should encourage major players in the palm oil industry to practice oil palm-livestock integration.
Intraoperative Indocyanine Green Retention Test of Left Hemiliver in Decision-Making for Patients With Hepatocellular Carcinoma Undergoing Right Hepatectomy
Authors: Yu Tingdong , Ye Xinping , Wen Zhang , Zhu Guangzhi , Su Hao , Han Chuangye , Huang Ketuan , Qin Wei , Liao Xiwen , Yang Chengkun , Liu Zhen , Wang Xiangkun , Xu Banghao , Su Ming , Lv Zili , Lau Wan Yee , Peng Tao
Published: September 2021

Introduction: The aim of this study was to select qualified patients with hepatocellular carcinoma (HCC) who underwent right hepatectomy (RH) via intraoperative indocyanine green retention test at 15 min (ICG-R15) of the left hemiliver, which prevents severe posthepatectomy liver failure (PHLF).

Methods: Twenty HCC patients who were preoperatively planned to undergo RH were enrolled. Intraoperative ICG-R15 of left hemiliver was measured after the right Glissonean pedicle was completely blocked. Patients then underwent RH if intraoperative ICG-R15 was ≤ 10%. Otherwise, patients underwent staged RH (SRH), either associating liver partitioning and portal vein ligation for staged hepatectomy (ALPPS) or portal vein ligation (PVL), followed by stage-2 RH. The comparison group consisted of patients with a ratio of standard left liver volume (SLLV) of > 40% and preoperative ICG-R15 ≤ 10% who underwent RH. The clinical outcomes of these two groups were compared.

Results: Of the 20 patients, six underwent stage-1 RH, six underwent ALPPS, five underwent PVL followed by stage-2 RH, and three failed to proceed to stage-2 RH after PVL. No significant differences were found among the 17 patients who underwent stage-1 or stage-2 RH in the study group, the 19 patients in the comparison group, the 11 patients in the stage-2 RH group, and the six patients in the stage-1 RH group in incidences of PHLF, postoperative complications, hospital stay, and HCC recurrence within 1 year after RH. Compared with the stage-1 ALPPS group, the mean operative time and blood loss of the stage-1 PVL group were significantly less (p <0.001 and p = 0.022, respectively). The stage-1 PVL group had a significantly longer waiting-time (43.4 vs. 14.0 days, p = 0.016) than the stage-1 ALPPS group to proceed to stage-2 RH. After stage-2 RH, tumor recurrence within 1 year was 20% (1/5) in patients after PVL and 50% (3/6) after stage-1 ALPPS.

Conclusions: Intraoperative ICG-R15 ≤ 10% of left hemiliver was valuable in intraoperative decision-making for patients who were planned to undergo RH. There is a possibility that stage-1 PVL might help to select patients with more favorable biological behavior to undergo stage-2 RH.
Association Between Postoperative Thrombocytopenia and Outcomes After Coronary Artery Bypass Grafting Surgery
Authors: Li Jinghang , Yu Dongmin , Song Yuanyuan , Cheang Iokfai , Wang Xiaowei
Published: September 2021

Objectives: The effect of postoperative thrombocytopenia on adverse events among coronary artery bypass graft (CABG) patients remains unclear. This study aims to investigate the association between postoperative thrombocytopenia and perioperative outcomes of CABG.

Methods: This is a retrospective study with MIMIC-III (Medical Information Mart for Intensive Care III) database. Adult patients who underwent CABG were included to analyze the impact of thrombocytopenia in patients' outcomes. Postoperative thrombocytopenia was defined as a platelet count <100 × 109/L on the first day after CABG surgery. A multivariable logistic regression analysis was utilized to adjust the effect of thrombocytopenia on outcomes for baseline and covariates, and to determine the association with outcomes.

Results: A total of 4,915 patients were included, and postoperative thrombocytopenia occurred in 696 (14.2%) patients. Postoperative thrombocytopenia was not associated with increased 28-day mortality (OR 0.75; 95% CI 0.33–1.72; P = 0.496) or in-hospital mortality (OR 0.75; 95% CI 0.34–1.63; P = 0.463) after adjusting for confounders. Regarding the secondary outcomes, it was associated with a higher risk of a prolonged stay in the intensive care unit (OR 1.53; 95% CI 1.18–1.97; P = 0.001), prolonged hospital stays (OR 1.58; 95% CI 1.21–2.06; P = 0.001), prolonged mechanical ventilation time (OR 1.67; 95% CI 1.14–2.44; P = 0.009), and a trend toward increased occurrence of massive bleeding (OR 1.41; 95% CI 1.00–2.01; P = 0.054). There was no significant association between an increased risk of prolonged vasopressor use and the continuous renal replacement therapy rate.

Conclusions: Postoperative thrombocytopenia was associated with prolonged ICU and hospital stays but not with increased perioperative mortality among CABG patients.
First Detection and Genome Sequencing of SARS-CoV-2 Lambda (C.37) Variant in Symptomatic Domestic Cats in Lima, Peru
Authors: Schiaffino Francesca , Ferradas Cusi , Jara Luis M. , Salvatierra Guillermo , Dávila-Barclay Alejandra , Sanchez-Carrion Camila , Ulloa Alexandra , Mascaro Lucero , Pajuelo Monica J. , Guevara Sarmiento Luis , Fernandez Manolo , Zimic Mirko
Published: September 2021

The role of domestic cats in the dynamics of SARS-CoV-2 remains poorly characterized, especially in epidemiologic contexts of countries with high viral transmission. Here, we report the first evidence of SARS-CoV-2 Lambda variant of interest in symptomatic domestic cats whose owners were diagnosed with COVID-19 in Lima, Peru, providing evidence that transmission of this new variant in domestic cats is occurring. More epidemiological studies are required to further characterize the role of domestic animals in the transmission dynamics of SARS-CoV-2.
Growth, Mineral Nutrients, Photosynthesis and Related Physiological Parameters of Citrus in Response to Nitrogen Deficiency
Authors: Huang Wei-Tao , Xie Yi-Zhi , Chen Xu-Feng , Zhang Jiang , Chen Huan-Huan , Ye Xin , Guo Jiuxin , Yang Lin-Tong , Chen Li-Song
Published: September 2021

Limited data are available on the physiological responses of Citrus to nitrogen (N) deficiency. ‘Xuegan’ (Citrus sinensis (L.) Osbeck) and ‘Shantian pummelo’ (Citrus grandis (L.) Osbeck) seedlings were fertilized with nutrient solution at a N concentration of 0, 5, 10, 15 or 20 mM for 10 weeks. N deficiency decreased N uptake and N concentration in leaves, stems and roots and disturbed nutrient balance and homeostasis in plants, thus inhibiting plant growth, as well as reducing photosynthetic pigment levels and impairing thylakoid structure and photosynthetic electron transport chain (PETC) in leaves, hence lowering CO2 assimilation. The imbalance of nutrients intensified N deficiency’s adverse impacts on biomass, PETC, CO2 assimilation and biosynthesis of photosynthetic pigments. Citrus displayed adaptive responses to N deficiency, including (a) elevating the distributions of N and other elements in roots, as well as root dry weight (DW)/shoot DW ratio and root-surface-per-unit volume and (b) improving photosynthetic N use efficiency (PNUE). In general, N deficiency had less impact on biomass and photosynthetic pigment levels in C. grandis than in C. sinensis seedlings, demonstrating that the tolerance of C. grandis seedlings to N deficiency was slightly higher than that of C. sinensis seedlings, which might be related to the higher PNUE of the former.
An Economic Model of Sustainable Development in the Russian Arctic: The Idea of Building Vertical Farms
Authors: Didenko Nikolay , Skripnuk Djamilia , Ilin Igor , Cherenkov Vitally , Tanichev Alexander , Kulik Sergei V.
Published: September 2021

The main aim of the article is to find out the key factors of sustainable development of the Russian Arctic, which is strategically significant for Russia. The academic literature was reviewed to find out the time dynamics of the references to the economic models suitable for achieving the goals of sustainable development, and there has been hyperbolic growth in the attention paid to similar problems all around the world. The article compares three relatively new economic models in order to understand which of them is the most applicable to the promotion of sustainable development in the Russian Arctic: (a) bioeconomy, (b) green economy and (c) circular economy. The analysis of the relevant sources shows that the model of the circular economy is preferable for the Russian Arctic. Most of the article is dedicated to understanding the sources and mechanisms of the circular economy. The schematic description of vertical greenhouses and possibility of using vertical farms are presented in the paper as an example of organization of local food production according to the principles of the circular economy. The article considers a modeled project of creating a vertical farm in the Russian Arctic and a simulated indicator—profit of the vertical farm.
Valorization of Winery Waste: Prokupac Grape Seed as a Source of Nutritionally Valuable Oil
Authors: Milanović Jelena , Malićanin Marko , Rakić Vesna , Jevremović Nenad , Karabegović Ivana , Danilović Bojana
Published: September 2021

Valorization of winery waste is a promising solution for waste management with additional benefit of bioactive compounds isolation. The aim of this study was to analyze the nutritional and bioactive potential of oil derived from Serbian autochthonous grape seed variety Prokupac and to compare it with known international grape varieties. Additionally, two extraction methods (ultrasound assisted extraction and cold pressing) were used in order to determine their influence on physico-chemical and nutritive characteristics of grape seed oil. Different methods, including determination of physicochemical properties, content of bioactive compounds, oxidative stability and antioxidative capacity were used for the characterization of grape seed oils. Results indicated that the higher yield was obtained by ultrasound assisted extraction compared to cold pressing. The application of ultrasound assisted extraction resulted in increased α-tocopherol content and better antioxidant capacity and oxidative stability of oil. Significant differences in bioactive composition were observed for Prokupac seed oil compared to the other grape varieties. Oil extracted from Prokupac grape seed had the lowest content of polyunsaturated and the highest content of saturated fatty acids, the highest content of α-tocopherol and consequently, the best antioxidative capacity. Prokupac grape seed has a great potential for isolation of nutritive and bioactive valuable oil.
Fall Recognition System to Determine the Point of No Return in Real-Time
Authors: Kim Bae Sun , Son Yong Ki , Jung Joonyoung , Lee Dong-Woo , Shin Hyung Cheol
Published: September 2021

In this study, we collected data on human falls, occurring in four directions while walking or standing, and developed a fall recognition system based on the center of mass (COM). Fall data were collected from a lower-body motion data acquisition device comprising five inertial measurement unit sensors driven at 100 Hz and labeled based on the COM-norm. The data were learned to classify which stage of the fall a particular instance belongs to. It was confirmed that both the representative convolutional neural network learning model and the long short-term memory learning model were performed within a time of 10 ms on the embedded platform (Jetson TX2) and the recognition rate exceeded 94%. Accordingly, it is possible to verify the progress of the fall during the unbalanced and falling steps, which are classified by subdividing the critical step in which the real-time fall proceeds with the output of the fall recognition model every 10 ms. In addition, it was confirmed that a real-time fall can be judged by specifying the point of no return (PONR) near the point of entry of the falling down stage.
Effect of the Non-Immunosuppressive MPT Pore Inhibitor Alisporivir on the Functioning of Heart Mitochondria in Dystrophin-Deficient mdx Mice
Authors: Dubinin Mikhail V. , Starinets Vlada S. , Talanov Eugeny Yu. , Mikheeva Irina B. , Belosludtseva Natalia V. , Serov Dmitriy A. , Tenkov Kirill S. , Belosludtseva Evgeniya V. , Belosludtsev Konstantin N.
Published: September 2021

Supporting mitochondrial function is one of the therapeutic strategies that improve the functioning of skeletal muscle in Duchenne muscular dystrophy (DMD). In this work, we studied the effect of a non-immunosuppressive inhibitor of mitochondrial permeability transition pore (MPTP) alisporivir (5 mg/kg/day), reducing the intensity of the necrotic process and inflammation in skeletal muscles on the cardiac phenotype of dystrophin-deficient mdx mice. We found that the heart mitochondria of mdx mice show an increase in the intensity of oxidative phosphorylation and an increase in the resistance of organelles to the MPT pore opening. Alisporivir had no significant effect on the hyperfunctionalization of the heart mitochondria of mdx mice, and the state of the heart mitochondria of wild-type animals did not affect the dynamics of organelles but significantly suppressed mitochondrial biogenesis and reduced the amount of mtDNA in the heart muscle. Moreover, alisporivir suppressed mitochondrial biogenesis in the heart of wild-type mice. Alisporivir treatment resulted in a decrease in heart weight in mdx mice, which was associated with a significant modification of the transmission of excitation in the heart. The latter was also noted in the case of WT mice treated with alisporivir. The paper discusses the prospects for using alisporivir to correct the function of heart mitochondria in DMD.
Histologic Transformation in EGFR-Mutant Lung Adenocarcinomas: Mechanisms and Therapeutic Implications
Authors: Pathak Ranjan , Villaflor Victoria M.
Published: September 2021

With the advent of potent EGFR tyrosine kinase inhibitors (TKIs), the treatment landscape of EGFR-mutant lung adenocarcinomas has changed drastically in recent years. However, the development of resistance to EGFR TKIs remains a critical barrier to improving survival in these patients. Histologic transformations to small cell lung carcinoma, large cell neuroendocrine carcinoma, squamous cell carcinoma, and the sarcomatoid phenotype have been increasingly recognized as important mechanisms of resistance. In this article, we summarize the known biological bases for such phenotypic switches in regard to EGFR TKIs and describe novel pathways that might be harnessed to develop effective novel therapies for patients with EGFR-mutant non-small cell lung cancers.
Optimization of a Handwriting Method by an Automated Ink Pen for Cost-Effective and Sustainable Sensors
Authors: Loghin Florin C. , Salmerón José F. , Lugli Paolo , Becherer Markus , Falco Aniello , Rivadeneyra Almudena
Published: September 2021

In this work, we present a do-it-yourself (DIY) approach for the environmental-friendly fabrication of printed electronic devices and sensors. The setup consists only of an automated handwriting robot and pens filled with silver conductive inks. Here, we thoroughly studied the fabrication technique and different optimized parameters. The best-achieved results were 300 mΩ/sq as sheet resistance with a printing resolution of 200 µm. The optimized parameters were used to manufacture fully functional electronics devices: a capacitive sensor and a RFID tag, essential for the remote reading of the measurements. This technique for printed electronics represents an alternative for fast-prototyping and ultra-low-cost fabrication because of both the cheap equipment required and the minimal waste of materials, which is especially interesting for the development of cost-effective sensors.
The Histopathology of the Appendix in Children at Interval Appendectomy
Authors: Pederiva Federica , Bussani Rossana , Shafiei Vennus , Codrich Daniela , Guida Edoardo , Schleef Jurgen
Published: September 2021

Whilst most surgeons agree that conservative treatment of appendiceal abscess in children is an adequate treatment, the need for subsequent interval appendectomy is still controversial. We analyzed the histopathology in interval appendectomy in search of signs of inflammation. All patients admitted between 2010 and 2017 with appendiceal abscess and scheduled for interval appendectomy were reviewed. The specimens were evaluated for grade of inflammation, type and distribution of cellular infiltrate, presence of necrosis or hemorrhage and infiltrate in the serosa. Forty-two patients had appendiceal abscess and were treated conservatively. Seven underwent emergent appendectomy. Thirty-three out of 35 patients underwent elective interval appendectomy. Thirty-two specimens were revised. Carcinoid tumor or other malignant lesions were not found. All of them presented some amount of inflammation, grade 1 to 2 in 53%, grade 3 to 4 in 47%. Twenty-five percent of the specimens had signs of necrosis accompanied by hemorrhage and in more than the half (53%) the infiltrate extended to the serosa. Conclusions: Although the appendix was mostly found not macroscopically inflamed intraoperatively, histology confirmed a certain grade of inflammation even months after the conservative treatment. No correlation was found between histopathologic findings and lapse of time between abscess treatment and interval appendectomy.
Is There a Role for Elective Early Upper Gastrointestinal Contrast Study in Neurologically Impaired Children following Laparoscopic Nissen Fundoplication?
Authors: Benkoe Thomas M. , Rezkalla Katrin , Wisgrill Lukas , Metzelder Martin L.
Published: September 2021

Assessment of discomfort as a sign for early postoperative complications in neurologically impaired (NI) children is challenging. The necessity of early routine upper gastrointestinal (UGI) contrast studies following laparoscopic Nissen fundoplication in NI children is unclear. We aimed to evaluate the role of scheduled UGI contrast studies to identify early postoperative complications following laparoscopic Nissen fundoplication in NI children. Data for laparoscopic Nissen fundoplications performed in NI children between January 2004 and June 2021 were reviewed. A total of 103 patients were included, with 60 of these being boys. Mean age at initial operation was 6.51 (0.11–18.41) years. Mean body weight was 16.22 (3.3–62.5) kg. Mean duration of follow up was 4.15 (0.01–16.65 years) years. Thirteen redo fundoplications (12.5%) were performed during the follow up period; eleven had one redo and two had 2 redos. Elective postoperative UGI contrast studies were performed in 94 patients (91%). Early postoperative UGI contrast studies were able to identify only one complication: an intrathoracal wrap herniation on postoperative day five, necessitating a reoperation on day six. The use of early UGI contrast imaging following pediatric laparoscopic Nissen fundoplication is not necessary as it does not identify a significant number of acute postoperative complications requiring re-intervention.
Early Deaths in Childhood Cancer in Romania—A Single Institution Study
Authors: Pruteanu Doina Paula , Olteanu Elena Diana , Cosnarovici Rodica , Mihut Emilia , Ecea Radu , Todor Nicolae , Nagy Viorica
Published: September 2021

(1) Background: Survival in childhood cancer has improved significantly over the last decades. However, early deaths (EDs) represent an important number of preventable deaths. Our aim was to provide more insight intoEDs in developing countries. (2) Methods: We conducted a retrospective analysis of patients aged 0–18 years with childhood cancer diagnosed between 1996 and 2008 and admitted in the Institute of Oncology “Prof. Dr. Ion Chiricuta” Cluj-Napoca (IOCN), Romania. After exclusion of patients (pts) older than 18 years at diagnosis, pts with a missing personal identification number and pts with unconfirmed diagnosis of malignancy, we included 783 pts in the final analysis. We defined ED as survival of less than one month after cancer diagnosis. We divided pts in groups according to age, major tumour categories and treatment time periods. (3) Results: ED was registered in 20 pts (2.55%). A total of 16EDs were registered in haematologic malignancies and 4 in solid tumours. Statistical analysis was performed on pts diagnosed with haematological malignancies. A statistically significant higher proportion of patients with performance status (PS) 3 and 4 died within one month after diagnosis (24.1%) than patients admitted with PS 0–2 (1%)—p < 0.01. We found no statistically significant difference regarding ED when comparing male versus female (p = 0.85), age at diagnosis or between the threeperiods of diagnosis (p = 0.7). (4) Conclusions: PS at admission is an important risk factor associated with ED in pts with haematologic malignancies. ED in our institution reflects frequent late presentation for medical care, late diagnosis and referral to specialised centres.
Does Maternal Mental Health and Maternal Stress Affect Preschoolers’ Behavioral Symptoms?
Authors: Santelices María Pía , Irarrázaval Matías , Jervis Pamela , Brotfeld Cristian , Cisterna Carla , Gallardo Ana María
Published: September 2021

(1) Background: Maternal stress and depression are considered risk factors in children’s socioemotional development, also showing high prevalence worldwide. (2) Method: Participants correspond to a longitudinal sample of 6335 mother/child pairs (18–72 months), who were surveyed in 2010 and then in 2012. The hypothesis was tested with SEM analysis, setting the child’s internalized/externalized problems as dependent variable, maternal depression as independent variable, and stress as a partial mediator. (3) Results: Both depression during pregnancy and recent depression has not only a direct effect on the internalizing and externalizing symptomatology of the child, but also an indirect effect through parental stress. Significant direct and indirect relationships were found. (4) Conclusions: Maternal depression and the presence of parental stress can influence children’s behavioral problems, both internalizing and externalizing.
Hyperbolically Symmetric Versions of Lemaitre–Tolman–Bondi Spacetimes
Authors: Herrera Luis , Di Prisco Alicia , Ospino Justo
Published: September 2021

We study fluid distributions endowed with hyperbolic symmetry, which share many common features with Lemaitre–Tolman–Bondi (LTB) solutions (e.g., they are geodesic, shearing, and nonconformally flat, and the energy density is inhomogeneous). As such, they may be considered as hyperbolic symmetric versions of LTB, with spherical symmetry replaced by hyperbolic symmetry. We start by considering pure dust models, and afterwards, we extend our analysis to dissipative models with anisotropic pressure. In the former case, the complexity factor is necessarily nonvanishing, whereas in the latter cases, models with a vanishing complexity factor are found. The remarkable fact is that all solutions satisfying the vanishing complexity factor condition are necessarily nondissipative and satisfy the stiff equation of state.