bluuebunny/crossref_metadata_2025 huggingface dataset:
Preferential X Chromosome Inactivation as a Mechanism to Explain Female Preponderance in Myasthenia Gravis
Authors: Nicolì Vanessa , Tabano Silvia Maria , Colapietro Patrizia , Maestri Michelangelo , Ricciardi Roberta , Stoccoro Andrea , Fontana Laura , Guida Melania , Miozzo Monica , Coppedè Fabio , Migliore Lucia
Published: April 2022

Myasthenia gravis (MG) is a neuromuscular autoimmune disease characterized by prevalence in young women (3:1). Several mechanisms proposed as explanations for gender bias, including skewed X chromosome inactivation (XCI) and dosage or sex hormones, are often involved in the development of autoimmunity. The skewed XCI pattern can lead to an unbalanced expression of some X-linked genes, as observed in several autoimmune disorders characterized by female predominance. No data are yet available regarding XCI and MG. We hypothesize that the preferential XCI pattern may contribute to the female bias observed in the onset of MG, especially among younger women. XCI analysis was performed on blood samples of 284 women between the ages of 20 and 82. XCI was tested using the Human Androgen Receptor Assay (HUMARA). XCI patterns were classified as random (XCI < 75%) and preferential (XCI ≥ 75%). In 121 informative patients, the frequency of skewed XCI patterns was 47%, significantly higher than in healthy controls (17%; p ≤ 0.00001). Interestingly, the phenomenon was observed mainly in younger patients (<45 years; p ≤ 0.00001). Furthermore, considering the XCI pattern and the other clinical characteristics of patients, no significant differences were found. In conclusion, we observed preferential XCI in MG female patients, suggesting its potential role in the aetiology of MG, as observed in other autoimmune diseases in women.
Case Review: Whole-Exome Sequencing Analyses Identify Carriers of a Known Likely Pathogenic Intronic BRCA1 Variant in Ovarian Cancer Cases Clinically Negative for Pathogenic BRCA1 and BRCA2 Variants
Authors: Alenezi Wejdan M. , Fierheller Caitlin T. , Revil Timothée , Serruya Corinne , Mes-Masson Anne-Marie , Foulkes William D. , Provencher Diane , El Haffaf Zaki , Ragoussis Jiannis , Tonin Patricia N.
Published: April 2022

Background: Detecting pathogenic intronic variants resulting in aberrant splicing remains a challenge in routine genetic testing. We describe germline whole-exome sequencing (WES) analyses and apply in silico predictive tools of familial ovarian cancer (OC) cases reported clinically negative for pathogenic BRCA1 and BRCA2 variants. Methods: WES data from 27 familial OC cases reported clinically negative for pathogenic BRCA1 and BRCA2 variants and 53 sporadic early-onset OC cases were analyzed for pathogenic variants in BRCA1 or BRCA2. WES data from carriers of pathogenic BRCA1 or BRCA2 variants were analyzed for pathogenic variants in 10 other OC predisposing genes. Loss of heterozygosity analysis was performed on tumor DNA from variant carriers. Results: BRCA1 c.5407-25T>A intronic variant, identified in two affected sisters and one sporadic OC case, is predicted to create a new splice effecting transcription of BRCA1. WES data from BRCA1 c.5407-25T>A carriers showed no evidence of pathogenic variants in other OC predisposing genes. Sequencing the tumor DNA from the variant carrier showed complete loss of the wild-type allele. Conclusions: The findings support BRCA1 c.5407-25T>A as a likely pathogenic variant and highlight the importance of investigating intronic sequences as causal variants in OC families where the involvement of BRCA1 is highly suggestive.
Dietary Restriction and Rapamycin Affect Brain Aging in Mice by Attenuating Age-Related DNA Methylation Changes
Authors: Yin Zhilei , Guo Xinpeng , Qi Yang , Li Pu , Liang Shujun , Xu Xiangru , Shang Xuequn
Published: April 2022

The fact that dietary restriction (DR) and long-term rapamycin treatment (RALL) can ameliorate the aging process has been reported by many researchers. As the interface between external and genetic factors, epigenetic modification such as DNA methylation may have latent effects on the aging rate at the molecular level. To understand the mechanism behind the impacts of dietary restriction and rapamycin on aging, DNA methylation and gene expression changes were measured in the hippocampi of different-aged mice. Examining the single-base resolution of DNA methylation, we discovered that both dietary restriction and rapamycin treatment can maintain DNA methylation in a younger state compared to normal-aged mice. Through functional enrichment analysis of genes in which DNA methylation or gene expression can be affected by DR/RALL, we found that DR/RALL may retard aging through a relationship in which DNA methylation and gene expression work together not only in the same gene but also in the same biological process. This study is instructive for understanding the maintenance of DNA methylation by DR/RALL in the aging process, as well as the role of DR and RALL in the amelioration of aging.
Partial fads2 Gene Knockout Diverts LC-PUFA Biosynthesis via an Alternative Δ8 Pathway with an Impact on the Reproduction of Female Zebrafish (Danio rerio)
Authors: Bláhová Zuzana , Franěk Roman , Let Marek , Bláha Martin , Pšenička Martin , Mráz Jan
Published: April 2022

The zebrafish (Danio rerio) genome contains a single gene fads2 encoding a desaturase (FADS2) with both Δ6 and Δ5 activities, the key player in the endogenous biosynthesis of long-chain polyunsaturated fatty acids (LC-PUFAs), which serve essential functions as membrane components, sources of energy and signaling molecules. LC-PUFAs include the precursors of eicosanoids and are thus predicted to be indispensable molecules for reproductive health in virtually all vertebrates. In mice, an amniotic vertebrate, fads2 deletion mutants, both males and females, have been confirmed to be sterile. In anamniotic vertebrates, such as fish, there is still no information available on the reproductive (in)ability of fads2 mutants, although zebrafish have become an increasingly important model of lipid metabolism, including some aspects of the generation of germ cells and early embryonic development. In the present study, we apply the CRISPR/Cas9 genome editing system to induce mutations in the zebrafish genome and create crispants displaying a degree of fads2 gene editing within the range of 50–80%. Focusing on adult G0 crispant females, we investigated the LC-PUFA profiles of eggs. Our data suggest an impaired pathway of the LC-PUFA biosynthesis of the ω6 and ω3 series in the first-rate limiting steps of the conversion of linoleic acid (LA) into γ-linolenic acid (GLA), and α-linolenic acid (ALA) into stearidonic acid (SDA), respectively, finally resulting in bad-quality eggs. Our data suggest the existence of an alternative Δ8 pathway, which bypasses the first endogenous LC-PUFA biosynthetic step in zebrafish in vivo, and suggest that the zebrafish bifunctional FADS2 enzyme is actually a trifunctional Δ6/Δ5/Δ8 desaturase.
The Impact of Sex, Circadian Disruption, and the ClockΔ19/Δ19 Genotype on Alcohol Drinking in Mice
Authors: Rizk Abanoub Aziz , Jenkins Bryan W. , Al-Sabagh Yasmine , Hamidullah Shahnaza , Reitz Cristine J. , Rasouli Mina , Martino Tami A. , Khokhar Jibran Y.
Published: April 2022

Shift work is associated with increased alcohol drinking, more so in males than females, and is thought to be a coping mechanism for disrupted sleep cycles. However, little is presently known about the causal influence of circadian rhythm disruptions on sex differences in alcohol consumption. In this study, we disrupted circadian rhythms in female and male mice using both environmental (i.e., shifting diurnal cycles) and genetic (i.e., ClockΔ19/Δ19 mutation) manipulations, and measured changes in alcohol consumption and preference using a two-bottle choice paradigm. Alcohol consumption and preference, as well as food and water consumption, total caloric intake, and weight were assessed in adult female and male ClockΔ19/Δ19 mutant mice or wild-type (WT) litter-mates, housed under a 12-hour:12-hour light:dark (L:D) cycle or a shortened 10-hour:10-hour L:D cycle. Female WT mice (under both light cycles) increased their alcohol consumption and preference over time, a pattern not observed in male WT mice. Compared to WT mice, ClockΔ19/Δ19 mice displayed increased alcohol consumption and preference. Sex differences were not apparent in ClockΔ19/Δ19 mice, with or without shifting diurnal cycles. In conclusion, sex differences in alcohol consumption patterns are evident and increase with prolonged access to alcohol. Disrupting circadian rhythms by mutating the Clock gene greatly increases alcohol consumption and abolishes sex differences present in WT animals.
Characterization of Altered Molecular Pathways in the Entorhinal Cortex of Alzheimer’s Disease Patients and In Silico Prediction of Potential Repurposable Drugs
Authors: Fagone Paolo , Mangano Katia , Martino Gabriella , Quattropani Maria Catena , Pennisi Manuela , Bella Rita , Fisicaro Francesco , Nicoletti Ferdinando , Petralia Maria Cristina
Published: April 2022

Alzheimer’s disease (AD) is the most common cause of dementia worldwide and is characterized by a progressive decline in cognitive functions. Accumulation of amyloid-β plaques and neurofibrillary tangles are a typical feature of AD neuropathological changes. The entorhinal cortex (EC) is the first brain area associated with pathologic changes in AD, even preceding atrophy of the hippocampus. In the current study, we have performed a meta-analysis of publicly available expression data sets of the entorhinal cortex (EC) in order to identify potential pathways underlying AD pathology. The meta-analysis identified 1915 differentially expressed genes (DEGs) between the EC from normal and AD patients. Among the downregulated DEGs, we found a significant enrichment of biological processes pertaining to the “neuronal system” (R-HSA-112316) and the “synaptic signaling” (GO:0099536), while the “regulation of protein catabolic process” (GO:00042176) and “transport of small molecules” (R-HSA-382551) resulted in enrichment among both the upregulated and downregulated DEGs. Finally, by means of an in silico pharmacology approach, we have prioritized drugs and molecules potentially able to revert the transcriptional changes associated with AD pathology. The drugs with a mostly anti-correlated signature were: efavirenz, an anti-retroviral drug; tacrolimus, a calcineurin inhibitor; and sirolimus, an mTOR inhibitor. Among the predicted drugs, those potentially able to cross the blood-brain barrier have also been identified. Overall, our study found a disease-specific set of dysfunctional biological pathways characterizing the EC in AD patients and identified a set of drugs that could in the future be exploited as potential therapeutic strategies. The approach used in the current study has some limitations, as it does not account for possible post-transcriptional events regulating the cellular phenotype, and also, much clinical information about the samples included in the meta-analysis was not available. However, despite these limitations, our study sets the basis for future investigations on the pathogenetic processes occurring in AD and proposes the repurposing of currently used drugs for the treatment of AD patients.
The Length of Leukocyte and Femoral Artery Telomeres in Patients with Peripheral Atherosclerosis
Authors: Boniewska-Bernacka Ewa , Pańczyszyn Anna , Hobot Jacek , Donizy Piotr , Ziembik Zbigniew , Goc Anna , Klinger Marian
Published: April 2022

The length of telomeres (TLs) that protect chromosome ends may reflect the age of cells as well as the degree of genetic material damage caused by external factors. Since leukocyte telomere length is associated with cardiovascular diseases, the aim of this study was to evaluate whether leukocyte TL reflects femoral artery wall telomeres of patients with atherosclerosis and lower limb ischemia. Samples of femoral artery wall and blood were collected from 32 patients qualified to surgical revascularization. The analysis included blood and artery wall telomere length measurement and biochemical parameters. The study indicated that there was a moderate correlation between artery wall TL and leukocyte TL. Leukocyte TL was, on average, two times shorter than artery wall TL and correlated with the number of white blood cells. In turn, artery TL was impacted by total cholesterol level. The results suggest that the length of leukocyte telomeres may reflect artery wall TL and indirectly reflect the processes taking place in the artery wall in patients with atherosclerosis.
The Role of Epigenetic Modifications in Late Complications in Type 1 Diabetes
Authors: Čugalj Kern Barbara , Trebušak Podkrajšek Katarina , Kovač Jernej , Šket Robert , Jenko Bizjan Barbara , Tesovnik Tine , Debeljak Maruša , Battelino Tadej , Bratina Nataša
Published: April 2022

Type 1 diabetes is a chronic autoimmune disease in which the destruction of pancreatic β cells leads to hyperglycemia. The prevention of hyperglycemia is very important to avoid or at least postpone the development of micro- and macrovascular complications, also known as late complications. These include diabetic retinopathy, chronic renal failure, diabetic neuropathy, and cardiovascular diseases. The impact of long-term hyperglycemia has been shown to persist long after the normalization of blood glucose levels, a phenomenon known as metabolic memory. It is believed that epigenetic mechanisms such as DNA methylation, histone modifications, and microRNAs, play an important role in metabolic memory. The aim of this review is to address the impact of long-term hyperglycemia on epigenetic marks in late complications of type 1 diabetes.
Pathogenesis of Type 1 Diabetes: Established Facts and New Insights
Authors: Zajec Ana , Trebušak Podkrajšek Katarina , Tesovnik Tine , Šket Robert , Čugalj Kern Barbara , Jenko Bizjan Barbara , Šmigoc Schweiger Darja , Battelino Tadej , Kovač Jernej
Published: April 2022

Type 1 diabetes (T1D) is an autoimmune disease characterized by the T-cell-mediated destruction of insulin-producing β-cells in pancreatic islets. It generally occurs in genetically susceptible individuals, and genetics plays a major role in the development of islet autoimmunity. Furthermore, these processes are heterogeneous among individuals; hence, different endotypes have been proposed. In this review, we highlight the interplay between genetic predisposition and other non-genetic factors, such as viral infections, diet, and gut biome, which all potentially contribute to the aetiology of T1D. We also discuss a possible active role for β-cells in initiating the pathological processes. Another component in T1D predisposition is epigenetic influences, which represent a link between genetic susceptibility and environmental factors and may account for some of the disease heterogeneity. Accordingly, a shift towards personalized therapies may improve the treatment results and, therefore, result in better outcomes for individuals in the long-run. There is also a clear need for a better understanding of the preclinical phases of T1D and finding new predictive biomarkers for earlier diagnosis and therapy, with the final goal of reverting or even preventing the development of the disease.
Earthquake Nowcasting with Deep Learning
Authors: Fox Geoffrey Charles , Rundle John B. , Donnellan Andrea , Feng Bo
Published: April 2022

We review previous approaches to nowcasting earthquakes and introduce new approaches based on deep learning using three distinct models based on recurrent neural networks and transformers. We discuss different choices for observables and measures presenting promising initial results for a region of Southern California from 1950–2020. Earthquake activity is predicted as a function of 0.1-degree spatial bins for time periods varying from two weeks to four years. The overall quality is measured by the Nash Sutcliffe efficiency comparing the deviation of nowcast and observation with the variance over time in each spatial region. The software is available as open source together with the preprocessed data from the USGS.
40 Years Later: New Perspectives on the 23 November 1980, Ms 6.9, Irpinia-Lucania Earthquake
Authors: Porfido Sabina , Alessio Giuliana , Gaudiosi Germana , Nappi Rosa , Michetti Alessandro Maria
Published: April 2022

After more than forty years since the 1980 Irpinia-Lucania earthquake, with this Special Issue “The 23 November 1980 Irpinia-Lucania, Southern Italy Earthquake: Insights and Reviews 40 Years Later” we revisit this milestone geological and seismological event, bringing together the latest views and news on this earthquake, with the aim of improving the dissemination of wide-ranging information on this remarkable case history [...]
Examining Rock Engineering Knowledge through a Philosophical Lens
Authors: Elmo Davide , Mitelman Amichai , Yang Beverly
Published: April 2022

This paper presents a philosophical examination of classical rock engineering problems as the basis to move from traditional knowledge to radical (innovative) knowledge. While this paper may appear abstract to engineers and geoscientists more accustomed to case studies and practical design methods, the aim is to demonstrate how the analysis of what constitutes engineering knowledge (what rock engineers know and how they know it) should always precede the integration of new technologies into empirical disciplines such as rock engineering. We propose a new conceptual model of engineering knowledge that combines experience (practical knowledge) and a priori knowledge (knowledge that is not based on experience). Our arguments are not a critique of actual engineering systems, but rather a critique of the (subjective) reasons that are invoked when using those systems, or to defend conclusions achieved using those systems. Our analysis identifies that rock engineering knowledge is shaped by cognitive biases, which over the years have created a sort of dogmatic barrier to innovation. It therefore becomes vital to initiate a discussion on the subject of engineering knowledge that can explain the challenges we face in rock engineering design at a time when digitalisation includes the introduction of machine algorithms that are supposed to learn from conditions of limited information.
Chimney Identification Tool for Automated Detection of Hydrothermal Chimneys from High-Resolution Bathymetry Using Machine Learning
Authors: Keohane Isaac , White Scott
Published: April 2022

Identifying the locations of hydrothermal chimneys across mapped areas of seafloor spreading ridges unlocks the ability to research questions about their correlations to geology, the cooling of the lithosphere, and deep-sea biogeography. We developed a Chimney Identification Tool (CIT) that utilizes a Convolutional Neural Network (CNN) to classify 1 m gridded AUV bathymetry and identify the locations of hydrothermal vent chimneys. A CNN is a type of Machine-Learning model that is able to classify raster data based on the shapes and textures in the input, making it ideal for this task. The criteria that have been used in previous manual classifications of chimneys have focused on the round base and spire shape of the features, and are not easily quantifiable. Machine-Learning techniques have previously been implemented with sonar data to classify seafloor geology, but this is the first application of these methods to hydrothermal systems. In developing the CIT, we compiled the bathymetry data from two rasters from the Endeavor Ridge—each gridded at a 1 m resolution—containing 34 locations of known hydrothermal chimneys, and from the 92° W segment of the Galapagos Spreading Center (GSC) containing 14. The CIT produced a primary group of outputs with 96% agreement with the manual classification; moreover, it correctly caught 29 of the 34 known chimneys from Endeavor and 10 of the 14 from the GSC. The CIT is trained to identify features with the characteristic shape of a hydrothermal vent chimney; therefore, it is susceptible to the misclassification of unusually shaped cases, given the limited training data. As a result, to provide the option of having a more inclusive application, the CIT also produced a secondary group of output locations with 61% agreement with the manual classification; moreover, it caught three of the four additional known chimneys from the GSC and four of the five from Endeavor. The CIT will be used in future investigations where an inventory of individual chimneys is important, such as the cataloguing of off-axis hydrothermal venting and the investigation of chimney distribution in connection to seafloor eruptions.
Relationship between Measured Aerobic Capacity and Total Energy Expenditure Obtained by the Doubly Labeled Water Method in Community-Dwelling, Healthy Adults Aged 81–94 Years
Authors: Yasukata Jun , Yamada Yosuke , Sagayama Hiroyuki , Higaki Yasuki , Tanaka Hiroaki
Published: April 2022

The doubly labeled water method is a gold-standard method for the measurement of total energy expenditure in daily life. We aimed to identify the relationship between measured aerobic capacity and total energy expenditure, activity energy expenditure, or physical activity level using the doubly labeled water method in adults of advanced old age. A total of 12 physically independent older adults (10 men and 2 women), aged 81–94 years, participated in this study. The aerobic capacity was evaluated according to the lactate threshold. Total energy expenditure under free-living conditions was assessed using the doubly labeled water method, and self-reported physical activity was obtained using the Japanese version of the International Physical Activity Questionnaire. The lactate threshold was significantly positively correlated with total energy expenditure, activity energy expenditure, and physical activity level after adjusting for age and sex. We found that the aerobic capacity of the lactate threshold was positively and independently correlated with total energy expenditure, activity energy expenditure, or physical activity level. The present results suggest that maintaining aerobic capacity is an important factor in preventing frailty, although further research is required.
Futurism without a Future: Thoughts on The Ministry of Time and Mirage (2015–2018)
Authors: Pueyo Zoco Victor M.
Published: April 2022

The future is not what it used to be. A new strain of futurism has taken over the stage of global science-fiction: one whose understanding of the future cannot be distinguished from its understanding of the present. Gone are the days when extraterrestrials in shiny, extravagant outfits mastered fascinating technologies that flirted with magic. Characters in Charlie Brooker’s Black Mirror (2015–2020) dress like us, and the dystopian technology they put up with is, for the most part, a technology that has existed for years. Armando Iannucci’s imagining of a space cruise for rich people in Avenue 5 (2020) overlaps with Elon Musk’s actual plans of sending wealthy tourists to the moon, while Albert Robida’s visionary téléphonoscope (1879) amounts to a sad reminder of our everyday Zoom call. Is not the current COVID-19 crisis the blueprint to the ultimate post-apocalyptic script? Spanish filmmaker Juan Antonio Bayona noted in a recent interview that Steve Soderbergh’s Contagion (2011), originally labeled as a sci-fi movie by IMDB, is now a drama according to the same internet portal. Science is not fiction anymore, which means at least two different things: that science has lost the power to convey the kind of awe that may be later turned into fiction, and that fiction seems to be unable to inspire a narrative of scientific or—broadly speaking—human progress. How can we retrieve the emancipatory value of progress in good old futuristic sci-fi when the future coincides with the present? What should cultural production look like to help us imagine an alternative to financial capitalism in the face of the impossibility of utopia? The answer, I will claim, resides in Franco Berardi’s concept of “futurability”. This paper explores the limits of this concept by reading side by side Javier Olivares’ and Pablo Olivares’ The Ministry of Time (2015) and Oriol Paulo’s Mirage (2018).
An Exploration of the Use and Impact of Preventive Measures on Skin Cancer
Authors: Hung Man , Beazer Isaac Rex , Su Sharon , Bounsanga Jerry , Hon Eric S. , Lipsky Martin S.
Published: April 2022

Background: Skin cancer is the most common form of cancer, and both clinical and epidemiological data link cumulative solar dosages and the number of sunburns to skin cancer. Each year, more than 5.4 million new cases of skin cancer are diagnosed, incurring a significant health and financial burden. Recommended preventive measures for skin cancer include the use of sunscreen, sun avoidance, and protective clothing. This study used a national database to examine the association of preventive measures with the prevalence of skin cancer, specifically analyzing the preventive measures of sunscreen use, staying in the shade, and wearing long-sleeved shirts. The second aim was to determine which characteristics, if any, correlated with using prevention measures. Methods: This study analyzed data from the National Health and Nutritional Examination Survey 2015–2016 cycle to examine the association of three preventive measures (using sunscreen, staying in the shade, and wearing long-sleeved shirts) with skin cancer. Logistic regression and chi-square tests were utilized to examine the relationship between skin cancer and these prevention methods. Results: Sunscreen use (OR = 3.752; p < 0.05) was statistically associated with a lower prevalence of skin cancer, while wearing long-sleeved shirts (OR = 6.911; p = 0.064) and staying in the shade (OR = 0.646; p = 0.481) did not emerge as factors significantly associated with a lower prevalence after controlling for gender, race/ethnicity, marital status, income, health insurance, and general health. Additionally, men and individuals of color were less likely to use sunscreen. Conclusion: Sunscreen use was associated with a lower prevalence of skin cancer, while wearing long-sleeved shirts and staying in the shade was not significantly linked to lower rates of skin cancer, suggesting that these measures may not be as effective as sunscreen for preventing skin cancer. Men and individuals of color were significantly less likely to use sunscreen. These findings can help guide future education efforts and research regarding skin cancer prevention and suggest the need to develop male-oriented programs to mitigate the gender disparity in employing sun-protection measures.
Nursing Experience of New Nurses Caring for COVID-19 Patients in Military Hospitals: A Qualitative Study
Authors: Kwon Young-Hoon , Han Hye-Ju , Park Eunyoung
Published: April 2022

This qualitative study explored the experiences of new nurses with less than one year of clinical experience in caring for COVID-19 patients in a military hospital. In-depth interviews were conducted with six new nurses working in a negative-pressure isolation unit of the Armed Forces Capital Hospital. Data were analyzed using the phenomenological method proposed by Colaizzi, and 12 themes were derived and classified into four clusters: burden of nursing in isolation units; hardship of nursing critically ill patients; efforts to perform nursing tasks; positive changes through patient care. The participants were anxious while caring for COVID-19 patients with severe illness due to a lack of clinical experience. Furthermore, the wearing of heavy personal protective equipment impeded communication with patients, leading to physical and psychological exhaustion. However, they tried to utilize their own know-how and provide the best nursing care, resulting in them gaining confidence. Participants were able to think critically and took pride in being military nursing professionals. This study is meaningful as it provides insight into the experiences of new military nurses who were rapidly dispatched during a national medical crisis. The results can be applied to develop future strategies aimed at improving new nurses’ competency in military hospitals.
The Relation of Surgical Procedures and Diagnosis Groups to Unplanned Readmission in Spinal Neurosurgery: A Retrospective Single Center Study
Authors: Sander Caroline , Oppermann Henry , Nestler Ulf , Sander Katharina , Fehrenbach Michael Karl , Wende Tim , von Dercks Nikolaus , Meixensberger Jürgen
Published: April 2022

Background: Unplanned readmission has gained increasing interest as a quality marker for inpatient care, as it is associated with patient mortality and higher economic costs. Spinal neurosurgery is characterized by a lack of epidemiologic readmission data. The aim of this study was to identify causes and predictors for unplanned readmissions related to index diagnoses and surgical procedures. Methods: In this study, from 2015 to 2017, spinal neurosurgical procedures were recorded for surgical and non-surgical treated patients. The main reasons for an unplanned readmission within 30 days following discharge were identified. Multivariate logarithmic regression revealed predictors of unplanned readmission. Results: A total of 1172 patient records were examined, of which 4.27% disclosed unplanned readmissions. Among the surgical patients, the readmission rate was 4.06%, mainly attributable to surgical site infections, while it was 5.06% for the non-surgical patients, attributable to uncontrolled pain. A night-time surgery presented as the independent predictive factor. Conclusion: In the heterogeneous group of spinal neurosurgical patients, stratification into diagnostic groups is necessary for statistical analysis. Degenerative lumbar spinal stenosis and spinal abscesses are mainly affected by unplanned readmission. The surgical procedure dorsal root ganglion stimulation is an independent predictor of unplanned re-hospitalizations, as is the timing of surgery.
The Effect of Adverse Mental Health and Resilience on Perceived Stress by Sexual Violence History
Authors: Anderson Katherine M. , Tsuyuki Kiyomi , Fernandez DeSoto Alexandra , Stockman Jamila K.
Published: April 2022

Sexual violence, including nonconsensual sexual initiation and rape, remains pervasive, with impacts including adverse mental health and dysregulated stress response. Resilience is a promising interventional target. To advance the science, we examined the potential for resilience as an interventional tool by estimating associations between resilience, adverse mental health, and perceived stress among women by sexual violence history and partner perpetration. We analyzed 2018–2020 baseline survey data from 65 women enrolled in a prospective case-control study of sexual violence and HIV susceptibility in San Diego, CA. Multiple linear regressions were performed to examine associations, stratified by sexual violence history. About half of women experienced nonconsensual sexual initiation and/or rape; half of rapes were partner-perpetrated. Post-traumatic stress disorder (PTSD) was significantly associated with perceived stress among survivors (in regressions with depression and resilience, nonconsensual initiation: β = 6.514, p = 0.003, R2 = 0.616; rape: β = 5.075, p = 0.030, R2 = 0.611). Resilience was associated with lower perceived stress for all women; the effect appeared stronger among survivors of sexual violence (nonconsensual initiation: β = −0.599, p < 0.001 vs. β = −0.452, p = 0.019; rape: β = −0.624, p < 0.001 vs. β = −0.421, p = 0.027). Partner perpetration of rape was not associated with perceived stress. Our findings support leveraging resilience and addressing PTSD to reduce perceived stress among women with lifetime experiences of sexual violence.
From Bench to Bedside—Implementing the New ABC Approach for Atrial Fibrillation in an Emergency Department Setting
Authors: Gupta Sophie , Lutnik Martin , Niederdöckl Jan , Schnaubelt Sebastian
Published: April 2022

Atrial fibrillation (AF) is a globally evolving medical challenge with, currently, 4% prevalence in the European Union’s population [...]
Healthcare Utilization and Adherence to Treatment Recommendations among Children with Type 1 Diabetes in Poland during the COVID-19 Pandemic
Authors: Grudziąż-Sękowska Justyna , Sękowski Kuba , Kobuszewski Bartosz
Published: April 2022

Type 1 diabetes mellitus (T1D) is, next to obesity and asthma, the most common chronic disease in children in Poland. The results of T1D treatment strongly depend on the patient’s compliance with therapeutic recommendations, which entails the use of necessary health services. Based on a retrospective analysis of the data on health services provided in 2016–2020 to over 15.5 thousand patients with T1D in Poland, we assessed the compliance of the actual model of treatment of T1D in children with the current guidelines. It was found that only about 50% of patients received the number of diabetes consultations corresponding to the recognized standards, with about 15% of children with T1D remaining outside the public healthcare system. In the case of many outpatient services (ophthalmological, neurological, mental health), the number of consultations was extremely low—one order of magnitude lower than in general population and dropped even lower in 2020. This shows that the health needs of children with T1D are not being met within the public healthcare system. The COVID-19 pandemic caused significant limitations in access to healthcare in Poland. Compared to the pre-pandemic period there was a significant decrease (−27% compared to 2019) in the number of hospitalizations, and a substantial increase (+22% compared to 2019) in the number of diabetic ketoacidoses (DKA) cases. The proportion of hospitalizations caused by DKA rose to 8.9% compared to 7.3% in 2019.
Physical Activity among Italian Adolescents: Association with Life Satisfaction, Self-Rated Health and Peer Relationships
Authors: Pierannunzio Daniela , Spinelli Angela , Berchialla Paola , Borraccino Alberto , Charrier Lorena , Dalmasso Paola , Lazzeri Giacomo , Vieno Alessio , Ciardullo Silvia , Nardone Paola
Published: April 2022

The aim of this study was to describe physical activity, both moderate-to-vigorous physical activity (MVPA) and vigorous physical activity (VPA), in a large nationally representative sample of Italian adolescents, aged 11, 13 and 15, and to evaluate if Italian adolescents were in line with the 2020 WHO recommendations. In order to assess the possible impact of physical activity on adolescent psychological and social wellbeing, the associations between MVPA and VPA with life satisfaction, self-rated health and peer relationships were also explored. Data from the 2018 Italian Health Behaviour in School-aged Children (HBSC) survey on 58,976 adolescents were analysed. Logistic regression analysis was used to investigate the association between physical activity and self-rated health, life satisfaction and peer relationships. The association of the Family Affluence Scale with self-rated health, life satisfaction and peer relationships was also studied. The results showed that the majority of Italian adolescents did not meet current physical activity WHO guidelines on MPVA. The prevalence of adolescents claiming to be involved “at least four or more times per week” in VPA was 29%. Overall, our findings highlighted a positive association between MVPA and VPA and life satisfaction, self-rated health and peer support. Data from this study underlined the need to encourage physical activity, especially among older adolescents and girls, who claimed lower levels of MPVA and VPA.
The Role of Surface Acting in the Relationship between Job Stressors, General Health and Need for Recovery Based on the Frequency of Interactions at Work
Authors: Sciotto Giulia , Pace Francesco
Published: April 2022

The aim of the study was to verify whether the frequency of face-to-face interactions with the public at work can reveal differences in how people react to emotional regulation demands. In particular, we investigated the mediating role of surface acting (a strategy of dealing with emotional dissonance) in the relationship between two typical job stressors (workload and mental load) and two outcomes closely related to work-related well-being: employees’ general health and the need for recovery. Prior studies investigating the detrimental effects of emotional dissonance mostly focused on service workers. However, in light of a survey conducted by the European Agency for Safety and Health at Work (2016) highlighting the growing psycho-social risk constituted by intense human interactions in the workplaces, even in unexpected categories of workers, we hypothesize that emotional demands may also be a concern for those who do not specifically interface with clients as part of their job duties. The results of the multi-group analysis of front-office (N = 734) and back-office (N = 436) Italian workers showed that surface acting fully mediates the relationship between workload and general health among back-office workers, while it only partially mediates this relationship among front-office workers. Furthermore, surface acting is positively associated with the need for recovery and negatively with general health, with higher values for back-office workers. The findings support the hypothesis that the emotional demands are not only a service worker issue and highlight the need to address emotional regulation strategies to enhance the quality of life in and outside the workplace for all employees.
Family Resilience and Adolescent Mental Health during COVID-19: A Moderated Mediation Model
Authors: Zhuo Ran , Yu Yanhua , Shi Xiaoxue
Published: April 2022

Background: The COVID-19 pandemic has impacted and is still impacting people’s lives, including physical and mental health. Family plays an important role in adolescent mental health due to the long staying at home. Aims: This paper aimed to investigate the impact of family resilience on adolescent mental health during the COVID-19 pandemic, and the mediation role of pandemic stress perception and the moderation role of meta-mood. Methods: A total of 2691 Chinese adolescents were recruited using convenient sampling. Their mental health, family resilience, pandemic stress perception and meta-mood were surveyed. Multivariate statistics were used to analyze the data. Results: Our results showed that (1) about 36.7% adolescents in our sample have some mental health problems; (2) family resilience can positively predict adolescent mental health, whereas pandemic stress perception can negatively predict mental health; (3) pandemic stress perception mediates the relationship between family resilience and adolescent mental health; (4) meta-mood moderates the relationship between family resilience and pandemic perception, i.e., the first half of the mediation role. Conclusions: Our results indicate that one can either improve family resilience or improve adolescents’ meta-mood to relieve adolescents’ mental health problems.
Exposure of Child Maltreatment Leads to a Risk of Mental Illness and Poor Prognosis in Taiwan: A Nationwide Cohort Study from 2000 to 2015
Authors: Huang Shi-Hao , Lin Iau-Jin , Yu Pi-Ching , Wang Bing-Long , Chung Chi-Hsiang , Huang Yao-Ching , Chien Wu-Chien , Sun Chien-An , Wu Gwo-Jang
Published: April 2022

Objective: To investigate whether children with maltreatment exposure were associated with the risk of psychiatric disorders, suicide, and death. Methods: A retrospective cohort study was conducted, with 1592 child maltreatment cases and 6368 comparison cohort (1:4) matched for gender, age, and index year, from the Longitudinal Generation Tracking Database (LGTD2000) sampled from Taiwan National Health Insurance Research Database (NHIRD) in 2000, backtracking between 2000–2015 in Taiwan. The stratified Cox regression model was used to compare the risk of developing a mental illness and poor prognosis during the 15 years of follow-up. Results: There were 473 in the cohort with child maltreatment (675.10 cases per 100,000 person years) and 1289 in the comparison cohort (453.82 cases per 100,000 person years) that developed mental illness and poor prognosis. The stratified Cox regression model revealed that the adjusted hazard ratio (HR) was 1.91 to 11.76 (p < 0.05) after adjusting for monthly income level, occupation, and CCI after violence. Conclusion: Exposure to child maltreatment is associated with the risk of psychiatric disorders, but not suicide or death. This finding could be a reminder for clinicians about the mental health problems in patients with child maltreatment.
Validation of the Organizational Dehumanization Scale in Spanish-Speaking Contexts
Authors: Ariño-Mateo Eva , Ramírez-Vielma Raúl , Arriagada-Venegas Matías , Nazar-Carter Gabriela , Pérez-Jorge David
Published: April 2022

The objective of this study is to validate Caesens, Stinglhamber, and Demoulin’s (2017) organizational dehumanization scale (ODS) in a Spanish-speaking sample. A sample of 422 employees (49.3% women and 50.7% men) from Chile answered an online questionnaire comprised of measures of organizational dehumanization and job satisfaction, organizational citizenship behaviors, and authentic leadership. To analyze the structure of the ODS, the sample was divided into two random subsamples and exploratory and confirmatory factor analyses were carried out. In addition, reliability and criterion validity were tested. As a result, the scale was composed of one factor. One item was eliminated due to its factor loading. The internal consistency was good (α = 0.92; ω = 0.92). The correlations between ODS, job satisfaction, organizational citizenship behaviors, and authentic leadership were statistically significant, from medium to high magnitude, indicating a reasonable degree of criterion validity. In conclusion, the Spanish version of the ODS shows adequate psychometric properties and can be useful for making progress on the understanding of organizational dehumanization and evaluating the organizational dehumanization in Spanish-speaking context.
Associations between Coping Profile and Work Performance in a Cohort of Japanese Employees
Authors: Otsuka Yuichiro , Itani Osamu , Matsumoto Yuuki , Kaneita Yoshitaka
Published: April 2022

This study aimed to investigate the effects of coping profiles on work performance. Data were collected during a 2-year prospective cohort study of 1359 employees in Japan. Participants completed a self-administered questionnaire in 2018 (T1; baseline) and again in 2020 (T2; followup; followup rate: 69.8%) to enable the assessment of work performance, perceived stress, and stress coping profiles at T1 and T2. Multivariate logistic regression models and causal mediation analysis were performed to identify the effects of coping profiles on work performance. Covariates included age, sex, company, job type, employment status, working hours, holidays, and lifestyle behaviors (e.g., smoking, sleep duration). A dysfunctional coping profile (β = −1.17 [95% CI, −2.28 to −0.06], p = 0.039) was negatively associated with work performance. Coping profiles of planning (β = 0.86 [95% CI, 0.07–1.66]) and self-blame (β = −1.33 [95% CI, −1.96 to −0.70], p < 0.001) were significantly associated with work performance. Dysfunctional coping, specifically, self-blame (β = −1.22 [95% CI, −1.83 to −0.61]), mediated the association between stress and work performance. Thus, some coping profiles may lead to an increase or decrease in work performance. The possible impact of coping strategies on workers’ productivity requires further exploration. Furthermore, information on effective coping profiles should be incorporated into occupational health examinations.
Penetrating Aortic Ulceration Treated with Castor Branched Aortic Stent Graft—A Case Series
Authors: Rizza Antonio , Negro Francesco , Palermi Stefano , Palmieri Cataldo , Murzi Michele , Credi Giovanni , Berti Sergio
Published: April 2022

Penetrating aortic ulceration (PAU) is an acute aortic syndrome similar to aortic dissection and intramural hematoma. It is the result of a tunica intima ulceration due to atherosclerotic disease. This clinical condition can lead to serious complications and a poor short-term prognosis, especially in high-surgical-risk patients. We report three cases of patients referred to “Ospedale del Cuore” of Massa (Italy) with PAU at the aortic arch-proximal descending aorta level who could not undergo surgical intervention. For the first time in Italy, we successfully treated these patients with a full percutaneous implantation of a Castor branched aortic stent graft. Our case series shows that this type of endovascular graft is an effective, safe, and feasible treatment for PAU involving a distal aortic arch and avoiding surgery and related complications.
Public Opinion on European Health Policy, Lessons from the COVID-19 Pandemic
Authors: Vasilescu Maria Denisa , Apostu Simona Andreea , Militaru Eva , Hysa Eglantina
Published: April 2022

Often, global crises, such as the COVID-19 pandemic, bring to light crucial weaknesses in political, economic, social and health systems. First, there are governments who formulate and implement policies and, second, there are the citizens who support them, thus contributing a great deal to their success. Our paper investigates the European citizens’ opinion on health policy, focusing on their preference for European health policy during the coronavirus pandemic. The paper uses bibliometric analysis, descriptive statistics, and logistic regression to discuss the public opinion on health policy, the factors of influence, the change in perspectives between 2020 and 2021, and the socio-demographic profile of those favorable for the development of a European health policy in response to the coronavirus pandemic. Our findings show that citizens from southern and central European countries are more likely to prioritize the development of a European health policy, as compared to Nordic countries. Between 2020 and 2021, pro-European health policy citizens profile changes and becomes clearer, from pensioners to young working age males with medium education. In general, people prioritizing a European health policy value health as the most important issue at a national level are generally satisfied with the European Union and do not trust their national government.
COVID-19 Related Fear, Risk Perceptions, and Behavioral Changes According to Level of Depression among Nursing Students
Authors: Kim Kyungmi , Jeong Hyesun , Lee Jongeun
Published: April 2022

Depression may have a negative impact on health behaviors during crisis situations, such as the COVID-19 pandemic. Accordingly, the present study aimed to investigate the effects of depression felt by nursing students on their infectious disease response. A total of 241 nursing students from two nursing colleges in Chungcheong Province was convenience sampled between 2 and 12 December 2020. The tools used in the study were the Patient Health Questionnaire-9 Korean version, Fear of COVID-19 Scale, COVID-19-related risk perceptions, and COVID-19 behavior changes. The depression group showed higher fear scores and lower behavioral change scores than the non-depression group. Such findings indicated that the depression group did not actively perform COVID-19-related preventive behaviors. With respect to the influencing factors of depression, depression scores were 2.28 times higher among sophomores than seniors; fear scores were 1.09 times higher in the depression group than the non-depression group; and behavioral change scores were 0.87 times lower in the depression group than the non-depression group. Based on the findings in the present study, it is necessary to screen nursing students with depression during disaster crisis situations, such as the COVID-19 pandemic, and provide active psychological support to such students for their mental health care.