bluuebunny/crossref_metadata_2025 huggingface dataset:
ARTEFATOS DE CONTROLADORIA E CONTABILIDADE GERENCIAL: ANÁLISE DOS ARTIGOS PUBLICADOS EM PERIÓDICOS BRASILEIROS DE CONTABILIDADE ENTRE OS ANOS 2009-2018
Authors: Mayk Jonhon de Carvalho Souza  , Kelly Aparecida Silva Jacques  , Lara Cristina Francisco de Almeida Fehr  , Vanessa Ferreira da Silva
Published: November 2020

O objetivo deste estudo é identificar o perfil bibliométrico dos artigos sobre artefatos deControladoria e Contabilidade Gerencial publicados entre 2009 e 2018 nos periódicosbrasileiros mais relevantes da área de contabilidade. Para o atendimento do objetivo proposto,foram coletados na plataforma de cada periódico os artigos cuja palavras-chavecorrespondessem ao foco desta pesquisa. Após a obtenção de uma amostra de 28 artigos, osdados obtidos foram tabulados e analisados sob a perspectiva de três leis bibliométricas: Lotka,Bradford e Zipt com o auxílio do Microsoft Excel e Microsoft Power BI. Os resultados dapesquisa demonstraram que o periódico ‘Enfoque – Reflexão Contábil’ foi o mais receptivo aosestudos ligados à temática. Além disso, destaca-se as autoras Beuren e Bortolucci Espejo comoas mais profícuas com estudos focados em artefatos gerenciais, além da forte participação depesquisadores na região Sul do país, em especial, vinculados à Universidade Regional deBlumenau (FURB). Os artefatos mais investigados ao longo do recorte temporal foramrespectivamente: Orçamento, Custeio por Absorção, Custeio ABC, Custeio Variável, Preços deTransferência e Benchmarking.
CARACTERÍSTICAS CORPORATIVAS SOBRE A DIVULGAÇÃO VOLUNTÁRIA DO EBITDA PELAS COMPANHIAS NA B3 EM 2018
Authors: Shaiane Pisa Kistner  , Orion Augusto Platt Neto
Published: November 2020

O objetivo do artigo é identificar as relações entre a divulgação do EBITDA pelas companhiaslistadas na B3, referente a 2018, e os setores de atuação, o tempo em bolsa, o nível degovernança e o total do ativo. O universo é composto por todas as companhias listadas na B3que estavam ativas e aptas à divulgação do EBITDA referente a 2018. A amostra inclui as 274companhias (97,5% do universo) que publicaram seus relatórios anuais. A pesquisa se baseounas observações de documentos corporativos e bases de dados derivadas de publicaçõesobrigatórias e voluntárias das sociedades abertas. Para a análise de dados, foram empregadastécnicas de estatística descritiva de associação e de correlação. A partir das análises estatísticasempregadas, abrangendo testes de hipóteses para um nível de confiança de 95%, concluiu-seque a divulgação de EBITDA pelas companhias: possui associação moderada com o tamanho(medido pelo ativo) e com a adesão a um nível de governança mais sofisticado (acima doTradicional); e não possui relação aparente com o tempo que as companhias estão listadas embolsa ou com os setores de atuação. Destaca-se, todavia, que estes resultados estão limitadosapenas ao ano de 2018, mesmo tendo grande abrangência do universo delimitado.
The economy of early Greek colonisation in the northern Aegean
Authors: Gimatzidis Stefanos
Published: November 2020

Greek colonisation has been one of the most popular topics in historical and archaeological debate already since the early modern period. Its study began in the historical context of early European colonialism, followed by rising liberalism during the early independence wars and French Revolution. It gained popularity after the industrial revolution in the Romantic period, when Greece and Rome were conceptualized as founders of Western culture and particularly in the age of New Imperialism that represented a new phase of colonial expansion faced with a growing awareness of social differences. Traditional perceptions of Greek colonisation were finally challenged during the most recent decolonisation period, particularly after the mid-20th century. All major historical events and intellectual movements of Modernity left their particular socio-cultural fingerprint on the studies of the Greek colonisation. One concept remained unchanged throughout this period. Greek colonisation was not fully emancipated – even in the decolonisation period – from preconceptions that emerged in the Zeitgeist of Western colonialism.
Specific features of the microbial resistance assessment in pig breeding enterprises
Authors: Isaeva Al'bina , Krivonogova Anna , Donnik Irina , Moiseeva Kseniya
Published: November 2020

Abstract. The aim of this work was to study the opportunistic microbiocenosis of a pig-breeding enterprise and analyze its resistance to antimicrobial drugs (AMR- status). Methods. For two years on the pig farm there were taken samples of microflora from the mucous membranes and teats of pregnant and farrowed sows, from the mucous membranes and skin of piglets of weaning, rearing, fattening groups; from equipment, fencing, surfaces and inventory in different technological areas. The microorganism isolates isolated from the samples were determined for their sensitivity to antibiotics, which are most often used in veterinary practice and feeding in this region. Results. It was established that the surveyed enterprise has an unfavorable AMR-status. The nucleus of the opportunistic microbiocenosis was represented by S. aureus, Ent. faecium and P. aeruginosa, which accounted for about 44 % of the isolated isolates, while the isolates of these microorganisms in 85 % of cases had reduced sensitivity or resistance to one or more antibiotics. Multidrug resistance was found mainly in S. aureus isolates, which in 16 % of cases were resistant to three antibiotics, and in 7 % of cases – to six studied antibiotics of various classes. Most often, multidrug-resistant staphylococcus aureus was detected in sows and piglets in the weaning phase. Also, during the research, there was found a high resistance of opportunistic microflora isolates to tetracycline, which had been used in this farm for more than three years. More than 10 % of tetracycline-resistant S. aureus and Ent. isolates were found on the skin and mucous membranes of fattening pigs. faecium, which indicated an increased risk of contamination of raw meat at the slaughter stage by microorganisms carrying genes for resistance to tetracyclines. The novelty of this work lies in obtaining relevant data on the AMR status of a pig-breeding facility and identifying the most unfavorable technological areas in terms of microbial resistance. The research is executed at the expense of a grant of the RSF (project No.18-16-00040).
Analysis of the inheritance of quantitative traits in the rice hybrid Kuboyar × Gagat
Authors: Kostylev Pavel , Krasnova E. , Aksenov A. , Balyukova E.
Published: November 2020

Abstract. Rice is one of the main food items in the world. White rice is mainly used, but there are also varieties with red, brown and black pericarp grains. This rice is much healthier. The article is devoted to the creation of new lines of rice with black pericarp. The purpose of the work is to study the inheritance of varying quantitative traits in an interspecific hybrid of rice Kuboyar × Gagat, with subsequent selection of isolated samples. Methods. Hybridization of these varieties was carried out in 2017. Mathematical processing of research data was performed Using the program Statistica 6. For genetic analysis, the program “Polygen A” was used by A. F. Merezhko (2005). The research was conducted in 2018–2019 on the basis of a Separate division “Proletarskoe” of the Rostov region. Scientific novelty. A genetic analysis of varying quantitative traits that affect the grain productivity of rice was performed, and a number of new patterns were established. Results. Inheritance of plant height in F2 hybrids was based on the type of overdomination of large trait values. The parent forms differed in the allelic state of the two pairs of genes. Along the length of the panicle, there was a partial negative dominance and monogenic differences in crossed varieties. According to the number of spikelets on the panicle, overdomination of a larger trait value and the interaction of two pairs of genes were established. By the mass of 1000 grains, partial dominance of large values of the trait and monogenic differences in the initial parent forms were established. There was no dominance in the length of the grain, and the parent forms differed by two pairs of genes. The grain width was dominated by smaller values of the trait, splitting was performed according to the monohybrid scheme. The selection of isolated samples forming a compact erect panicle and grains with a black pericarp was carried out for further selection work.
Um breve histórico das medidas socioeducativas em meio aberto: A efetividade do Sistema Nacional de Atendimento Socioeducativo (SINASE) no Centro de Referência Especializado em Assistência Social (CREAS) de Imperatriz – MA
Authors: Mendonça Lorenna Lima
Published: November 2020

Para que se chegasse ao entendimento deste tema, foi realizado um estudo sobre o histórico da infância e juventude no Brasil, ressaltando a “doutrina de situação irregular” que tinha como objetivo punir os menores infratores e as crianças pobres, até chegar a implantação da “doutrina de proteção integral” que buscou consolidar os direitos e deveres da criança e do adolescente, trazendo medidas socioeducativas para àqueles que cometerem ato infracional. E para ser realizada tal análise, foi utilizada a pesquisa bibliográfica em livros, monografias, artigos científicos, etc. Bem como foi realizada também uma pesquisa de campo diretamente no Centro de Referência Especializado em Assistência Social (CREAS) da cidade de Imperatriz – MA. Contudo, o presente artigo tem por objetivo trazer um melhor entendimento sobre o ato infracional e suas respectivas punições para crianças e adolescentes que cumprem medida socioeducativa em meio aberto, bem como apresentar como está a efetivação do Sistema Nacional de Atendimento Socioeducativo (SINASE) no Centro de Referência Especializado em Assistência Social (CREAS). A realização deste estudo mostrou-se imprescindível na medida em que traz uma reflexão sobre os limites da efetivação das medidas socioeducativas em meio aberto, mas sobre um enfoque que busca superar o reducionismo da visão punitiva e histórica de criminalização dos adolescentes em conflito com a lei em nosso país, materializada nos antigos códigos de menores de 1927 e 1979 a partir de uma visão saneatória.
Responsabilidade Internacional do estado e a violação dos Direitos Humanos sob o aspecto laboral em função da pandemia do Covid -19
Authors: Brito Clara Kelliany Rodrigues De , Matos André Pereira
Published: November 2020

Considerando a grande recessão da economia mundial, diversos estados por meio de seus governantes, visando o aumento dos índices econômicos por meio de uma corrida ávida por resultados, acabam violando diversos direitos humanos, passando por cima inclusive de direitos sociais que gozam de proteção internacional. Essa busca desenfreada por estabilidade econômica por meio do rápido crescimento se choca com o Direito do Trabalho que vem sofrendo de forma sistêmica diversas violações consagradas não só no Direito Interno como no Direito Internacional. O presente trabalho visa por meio de uma análise criteriosa demonstrar tais violações de direitos já consagrados internacionalmente em decorrência da pandemia do Coronavírus e a possível responsabilização internacional do Estado por tal conduta com base nas proteções internacionais de direitos humanos.
Efeito da melatonina sobre a morfofisiologia cardíaca de ratos induzidos a hiperlipidemia
Authors: Ferreira Fernanda Amorim , Paraíba Isabella Maria Rios , Elias Laíse de Souza
Published: November 2020

As hiperlipidemias são classificadas como alterações metabólicas e estão relacionadas com doenças cardiovasculares, as quais se destacam a doença aterosclerótica coronariana. As doenças cardiovasculares têm notável participação nas taxas de mortalidade a nível mundial e em virtude disso, vem sendo alvo de pesquisas que buscam inovação terapêutica. A melatonina é um hormônio produzido em fase de escuridão ambiental pela glândula pineal que desempenha função antioxidante, hipolipemiante, anti-inflamatória, entre outras, demonstrando assim, ser uma potente molécula no tratamento de doenças cardiovasculares. Assim, o objetivo deste trabalho consiste em avaliar o efeito da melatonina sobre o estresse oxidativo e parâmetros bioquímicos em ratos induzidos à hiperlipidemia. Para isso, foram utilizados 15 ratos albinos (Rattus norvegicus albinus) machos, com 150 dias de idade, procedentes do biotério do departamento de Morfologia e Fisiologia Animal da Universidade Federal Rural de Pernambuco. Esses animais foram mantidos em gaiolas, com alimentação e água ad libitum, temperatura de 22° C e iluminação artificial que estabelecerá um fotoperíodo de 12 horas claro e 12 horas escuro (ciclo invertido). Em conclusão, estudos demonstraram que a melatonina tem efeitos na redução da peroxidação lipídica, níveis de creatina quinase (CK) bem como na diminuição da glutationa reduzida. Como uma droga barata e bem tolerada, a melatonina pode ser uma nova opção terapêutica para doenças cardiovasculares e indivíduos com hiperlipidemia.
Um olhar sensível na prática pedagógica em espaços hospitalares: A andragogia
Authors: Alves Geania Moreira
Published: November 2020

O presente artigo tem como objetivo apresentar os benefícios do atendimento andragógico dentro da Pedagogia Hospitalar para auxiliar na promoção da saúde integral do adulto hospitalizado e os reflexos positivos tanto para o aluno-paciente como para o hospital e para a economia da cidade. Considera o papel importante da Pedagogia Hospitalar dentro do contexto histórico da educação e tem por base uma metodologia libertadora e humanitária que atua através de projetos e dialoga de maneira enriquecedora com os componentes curriculares instituídos pela Base Nacional Comum Curricular – BNCC para o desenvolvimento das competências e habilidades, do pensamento crítico e da afetividade do aluno. As práticas educacionais do século XXI pedem a inclusão imediata do adulto hospitalizado no atendimento da Pedagogia Hospitalar.
El papel de los incentivos fiscales a la inversión I+D+i en España
Authors: Palomares Moralles Pascual , Ripoll Alcón Joan
Published: November 2020

El propósito de este estudio es analizar el papel de los incentivos fiscales a la inversión en investigación, desarrollo e innovación (I+D+i) en España, y en particular, la relevancia de la figura legal Patent Box basada en la cesión de uso de activos intangibles.

Este artículo ilustra, a partir de un análisis comparativo, cómo el esquema Patent Box supone un ahorro fiscal evidente respecto aquella situación en la que no se hace uso del mismo. Este ahorro acaba erigiéndose en un incentivo fiscal efectivo en la promoción de proyectos de innovación empresarial, a diferencia de las deducciones fiscales, cuyo proceloso procedimiento administrativo y la fijación de un límite máximo de reducción de la cuota íntegra del impuesto de sociedades, que se ha revelado insuficiente, han tendido a minar su protagonismo como instrumento de ayuda indirecta a la financiación de la I+D+i.
An Algorithmic Approach to Solve Continuum Hypothesis
Authors: Kai Shun Mr. Lam
Published: November 2020

The continuum hypothesis has been unsolved for hundreds of years. In other words, can I answer it completely? By refuting the culturally responsible continuum [1], one can link the problem to the mathematical continuum, and it is possible to disproof the continuum hypothesis [2] . To go ahead a step, one may extend our mathematical system (by employing a more powerful set theory) and solve the continuum problem by three conditional cases. This event is sim-ilar to the status cases in the discriminant of solving a quadratic equation. Hence, my proposed al-gorithmic flowchart can best settle and depict the problem. From the above, one can further con-clude that when people extend mathematics (like set theory — ZFC) into new systems (such as Force Axioms), experts can solve important mathematical problems (CH). Indeed, there are differ-ent types of such mathematical systems, similar to ancient mathematical notation. Hence, different cultures have different ways of representation, which is similar to a Chinese saying: “different vil-lages have different laws.” However, the primary purpose of mathematical notation was initially to remember and communicate. This event indicates that the basic purpose of developing any new mathematical system is to help solve a natural phenomenon in our universe.
HUBUNGAN TINGKAT PENGETAHUAN IBU HAMIL DENGAN KEJADIAN HEPATITIS B DI PUSKESMAS BERINGIN KECAMATAN LUBAI KOTA PALEMBANG
Authors: Zulfian Zulfian , Setiawati Octa Reni , Sapitia Anisa
Published: November 2020

Hepatitis B merupakan salah satu penyakit menular berbahaya yang menyebabkan Kejadian Luar Biasa (KLB) dan kematian. Pemberian Kelengkapan imunisasi dasar pada anak seperti hepatitis B dipengaruhi oleh banyak faktor seperti tingkat pengetahuan dan dukungan keluarga. Penelitian ini merupakan penelitian bersifat deskriptif analitik dengan pendekatan cross-sectional yang dilakukan terhadap 52 responden. Analisis data menggunakan  uji Chi-Square dengan program SPSS 16.0. diperoleh ada hubungan yang bermakna antara  tingkat pengetahuan dengan kejadian hepatitis B dengan nilai p-value = 0,021. Ada hubungan yang bermakna antara  tingkat pengetahuan dengan kejadian hepatitis B (p-value=0.021).
On the Relationship between Investment Risk and Insurance Solvency Optimum
Authors: Szüle Borbála
Published: November 2020

Solvency is a key issue in the insurance sector. Investments can have significant risks, and a compelling research question is whether the solvency optimizing investment risk corresponds to the lowest possible risk level. This question is even more topical with some interest rates approaching very low levels in many countries. The paper aims to answer this question. Theoretical results suggest that if insurance risk and investment risk are uncorrelated, solvency optimizing investment portfolios with non-risk-free components may exist, and the level of optimal investment risk may inversely depend on the insurance portfolio size.
Inspired Thinking
Authors: Eguaras Gillian
Published: November 2020

Book Review
PANDEMİ (COVİD-19) SÜRECİNDEKİ UZAKTAN EĞİTİM UYGULAMALARINA İLİŞKİN LİSANSÜSTÜ ÖĞRENCİ GÖRÜŞLERİ
Authors: GENÇ Salih Zeki , ENGİN Gizem , YARDIM Tuğçe
Published: November 2020

Bu çalışmanın amacı covid-19 sürecinde uygulanan uzaktan eğitim uygulamalarının lisansüstü öğrencilerinin görüşlerine göre değerlendirilmesidir. Araştırma bir durum çalışmasıdır. Çalışmada Çanakkale Onsekiz Mart Üniversitesi Eğitim Bilimleri Enstitüsü’ne bağlı lisansüstü öğrenimine devam eden gönüllü 14 öğrenciden öğrenci günlükleri yoluyla ve bu 14 öğrencinin içinden 7 kişiyle yarı yapılandırılmış görüşme formları kullanılarak veriler toplanmıştır. İçerik analizi ve betimsel analiz kullanılarak veriler analiz edilmiştir. Araştırmanın sonucunda lisansüstü öğrencilerin uzaktan eğitim uygulamalarının verimini arttırdığını düşündükleri faktörler derslere fiziksel hazırlığın ortadan kalkması, ders kayıtlarının ve materyallerinin ulaşılabilir olması, öğretim elemanları ile iletişimin hızlanması, öğretim elemanlarının destekleyici tavır ve yeterlikleri, üniversitenin uzaktan eğitim uygulamalarına hızlı adapte oluşu, öğrencilerin nitelikleri, zaman-para-emek açısından ekonomik oluşu, ödevlerin öğrencileri derse hazırlaması/dersten kopmayı önlemesi, öğretimsel görevlere verilen geribildirimler, kaynaklara erişim kolaylığı sağlaması, online eğitimlere katılım fırsatı olarak ifade edilmiştir. Uzaktan eğitim süreci üzerinde olumsuz etki oluşturan faktörler ise derslerin bir günde yoğunlaşması, artan ödev yükü, aile üyelerinin gürültüsü/dışarıdan gelen uyaranlar, öğretimsel görevlerin yeterli açıklanmaması, teknolojiye aşırı maruz kalma, pandemi kaynaklı sorunlar, öğrenci nitelikleri, internet kaynaklı sıkıntılar, sistemsel-teknolojik sorunlar, yöntem ve tekniklerde tekdüzelik, sınıf mevcutlarının fazlalığı, ders içeriklerinin öğrenciler tarafından sunulması (kötü sunumlar) olarak belirtilmiştir. Uzaktan eğitimde yaşanan bu sorunları ifade eden çalışmalara alanyazında da rastlanmaktadır.
Policiamento comunitário: Análise da criminalidade no subúrbio de Salvador
Authors: Sobral Neto Rivaldo Ribeiro , Barbosa Murilo Jacques
Published: November 2020

O estudo pretende examinar os índices de segurança pública, no subúrbio ferroviário, com foco nos bairros de Coutos e Fazenda Coutos, como também, visando analisar outros bairros de Salvador que possuem ou não bases comunitárias, tendo como finalidade de externar a correlação da redução da criminalidade com o advento da polícia comunitária. Cuida-se de uma temática de grande relevância, visto que a segurança pública, além de propiciar a melhor qualidade de vida da população, é factível, sobretudo, para o fomento do desenvolvimento social e econômico, viabilizando, consequentemente, soluções para o enfrentamento da criminalidade. Realizou-se uma síntese histórica acerca da segurança pública, estando em consonância com o recomendado pela ONU (2012), ao prescrever que a segurança humana, através da conjugação das dimensões de paz, segurança e desenvolvimento, promoverá o fortalecimento dos direitos fundamentais. Destarte, foram apresentados alguns índices de segurança pública possibilitando descortinar e tornar cristalina se as bases comunitárias promoveram a diminuição da criminalidade. Neste exame, foi também observado que as bases comunitárias de Coutos e Fazenda Coutos se apresentaram com melhores resultados, quando comparado com outros bairros do Subúrbio de Salvador, acerca dos índices de criminalidade após a implementação das bases de policiamento comunitário.
Satisfação atlética e paixão em atletas brasileiras de handebol de elite
Authors: Faria Jaqueline Gazque , Contreira Andressa Ribeiro , Xavier Caroline Carneiro , Freitas Ana Flavia Lopes , Ribas Marcelen Lopes , Fiorese Lenamar , Vissoci João Ricardo Nickenig
Published: November 2020

Este estudo objetivou analisar a satisfação atlética e a paixão em atletas brasileiras de handebol. Foram sujeitos 88 atletas, participantes do Campeonato Brasileiro de Handebol Feminino Adulto 2016. Os instrumentos foram o Questionário de Satisfação do Atleta e a Escala da Paixão. Os dados foram analisados pelos testes Kolmogorov Smirnov, U de Mann Whitney e Coeficiente de Correlação de Spearman (p<0,05). As atletas de handebol apresentam-se mais satisfeitas nas dimensões dedicação pessoal (Md=5,45), contribuição equipe a tarefa (Md=5,03) e apresentaram paixão harmoniosa (Md=5,65). Foram encontradas diferenças (p<0,05) entre os níveis competitivos, indicando que as atletas de nível internacional são mais satisfeitas com o desempenho da equipe (Md=5,00) e utilização de habilidades (Md=5,40). Foram encontradas diferenças em todas as dimensões da satisfação em função do desempenho na competição (p<0,05), com valores superiores para as atletas medalhistas. As análises apontaram que para as atletas medalhistas houve correlação entre a satisfação e paixão (p<0,01), com destaque para a dimensão dedicação pessoal com a paixão harmoniosa (r=0,39) e paixão obsessiva (r=0,38). Já para as atletas não medalhistas os resultados evidenciaram correlações significativas (p<0,01) entre a paixão obsessiva e desempenho individual (r=0,47) e ética (r=0,45). A dimensão integração da equipe esteve correlacionada com a paixão harmoniosa e obsessiva (p>0,05). Concluiu-se que as atletas brasileiras de handebol de elite apresentam-se satisfeitas com as contribuições para equipe e com as ações do grupo para o sucesso esportivo refletindo sobre seu desempenho pessoal, além de apresentarem paixão harmoniosa pela modalidade, sendo mais evidente para as atletas medalhistas.
Abortamento de repetição: etiologia e cuidados
Authors: Barros Breno Pena , Barbosa Carolynne Hernandez , Santos Ellen Anny Pontes Moura dos , Costa Esmeson Pereira da , Lopes Graciana de Sousa , Ribeiro Roseane Gomes , Souza Welberson Soares de
Published: November 2020

Objetivo: discutir os principais aspectos relacionados ao abortamento de repetição e cuidados preventivos e caracterizar a importância da assistência do pré-natal na prevenção ao abortamento de repetição. Método: Trata-se de um estudo qualitativo de caráter explicativo através de revisão integrativa de literatura, que é um método que proporciona conhecimento e resultados de estudos na prática, com ampla abordagem metodológica incorporando conceitos, revisão de teorias, evidencias e analise de problemas metodológicos. Resultados: o aborto recorrente pode ser de diferentes etiologias, porém, 40 a 60% dos abortos recorrentes são de causas identificáveis ou idiopáticos pelo fato de não estarem ligados a nenhuma doença ou talvez por falta de investigação. Conclusão: as perdas gestacionais recorrentes são multifatoriais, podendo ocorrer por anormalidades nos cromossomos e útero, infecções e causas que podem ser consideradas como identificáveis por não terem ligação a uma doença especifica. Porém, a assistência pré-natal é de grande importância na prevenção ao aborto espontâneo de repetição, pois a mesma planeja a redução de riscos e garante o bem estar da mulher em seu período gravídico.
Reanatomização de dentes anterossuperiores e correção de diastema: relato de caso
Authors: Moss Olívia Breda , Peris Alessandra Rezende , Mitsui Fabio Hiroyuki Ogata , Rocha Lara Silva da
Published: November 2020

A presença de diastemas anterossuperiores pode acarretar problemas funcionais, estéticos e psicológicos. Com a evolução dos materiais odontológicos, a reabilitação destes casos utilizando resina composta de maneira direta facilitou o tratamento e possibilitou ótimos resultados. O presente trabalho visa relatar um caso clínico no qual se realizou um fechamento de diastema e reanatomização de dentes na região anterossuperior por meio de restauração direta em resina composta, priorizando a técnica conservadora com o objetivo de reestabelecer a estética e harmonia do sorriso, além da autoestima da paciente. Paciente do gênero feminino, 43 anos, relatou descontentamento com a aparência do seu sorriso em virtude da coloração e de um diastema localizado na região dos incisivos centrais. Realizou-se então o clareamento dental caseiro, enceramento diagnóstico, confecção do guia de silicone palatino, restaurações em resina composta, acabamento e polimento. A utilização das resinas compostas de maneira direta otimiza tempo clínico, apresenta melhor custo-benefício e não há a necessidade de desgaste da estrutura dental, obtendo desta forma um tratamento conservador e estético. A realização do enceramento diagnóstico possibilita uma previsibilidade do resultado, podendo o paciente junto ao cirurgião dentista visualizar e opinar sobre o mesmo quanto à forma e tamanho. Desta maneira, seguindo o protocolo restaurador de maneira criteriosa, alcançou-se o sucesso da técnica e do resultado final, atingindo os conceitos estéticos clínicos e as expectativas da paciente.
A New Set of Financial Instruments
Authors: Shirvani Abootaleb , Stoyanov Stoyan V. , Rachev Svetlozar T. , Fabozzi Frank J.
Published: November 2020

In complete markets there are risky assets and a riskless asset. It is assumed that the riskless asset and the risky asset are traded continuously in time and that the market is frictionless. In this paper, we propose a new method for hedging derivatives assuming that a hedger should not always rely on trading existing assets that are used to form a linear portfolio comprised of the risky asset, the riskless asset, and standard derivatives, but rather should design a set of specific, most-suited financial instruments for the hedging problem. We introduce a sequence of new financial instruments best suited for hedging jump-diffusion and stochastic volatility market models. The new instruments we introduce are perpetual derivatives. More specifically, they are options with perpetual maturities. In a financial market where perpetual derivatives are introduced, there is a new set of partial and partial-integro differential equations for pricing derivatives. Our analysis demonstrates that the set of new financial instruments together with a risk measure called the tail-loss ratio measure defined by the new instrument’s return series can be potentially used as an early warning system for a market crash.
Gut Microbiota Status in COVID-19: An Unrecognized Player?
Authors: Donati Zeppa Sabrina , Agostini Deborah , Piccoli Giovanni , Stocchi Vilberto , Sestili Piero
Published: November 2020

Infection with the SARS-CoV-2 virus causes cardiopulmonary and vascular complications, ranging in severity. Understanding the pathogenic mechanisms of the novel SARS-CoV2 infection and progression can provide potential novel targets for its prevention and/or treatment. Virus microbiota reciprocal interactions have been studied in a variety of viral infections. For example, the integrity of Coronavirus particles can be disrupted by surfactin, a bacterial surface molecule that targets other viruses, including that of influenza A. In this light, intestinal microbiota likely influences COVID-19 virulence, while from its side SARS-CoV-2 may affect the intestinal microbiome promoting dysbiosis and other deleterious consequences. Hence, the microbiota pre-existing health status and its alterations in the course of SARS-CoV-2 infection, are likely to play an important, still underscored role in determining individual susceptibility and resilience to COVID-19. Indeed, the vast majority of COVID-19 worst clinical conditions and fatalities develop in subjects with specific risk factors such as aging and the presence of one or more comorbidities, which are intriguingly characterized also by unhealthy microbiome status. Moreover, these comorbidities require complex pharmacological regimens known as “polypharmacy” that may further affect microbiota integrity and worsen the resilience to viral infections. This complex situation may represent a further and underestimated risk with regard to COVID-19 clinical burden for the elderly and comorbid people. Here, we discuss the possible biological, physiopathological, and clinical implications of gut microbiota in COVID-19 and the strategies to improve/maintain its healthy status as a simple and adjunctive strategy to reduce COVID-19 virulence and socio-sanitary burden.
Role of the PE/PPE Family in Host–Pathogen Interactions and Prospects for Anti-Tuberculosis Vaccine and Diagnostic Tool Design
Authors: Qian Jianing , Chen Run , Wang Honghai , Zhang Xuelian
Published: November 2020

The pe/ppe genes are found in pathogenic, slow-growing Mycobacterium tuberculosis and other M. tuberculosis complex (MTBC) species. These genes are considered key factors in host-pathogen interactions. Although the function of most PE/PPE family proteins remains unclear, accumulating evidence suggests that this family is involved in M. tuberculosis infection. Here, we review the role of PE/PPE proteins, which are believed to be linked to the ESX system function. Further, we highlight the reported functions of PE/PPE proteins, including their roles in host cell interaction, immune response regulation, and cell fate determination during complex host-pathogen processes. Finally, we propose future directions for PE/PPE protein research and consider how the current knowledge might be applied to design more specific diagnostics and effective vaccines for global tuberculosis control.
Multi-Omics Analysis Reveals Novel Subtypes and Driver Genes in Glioblastoma
Authors: Yuan Yang , Qi Pan , Xiang Wang , Yanhui Liu , Yu Li , Qing Mao
Published: November 2020

Glioblastoma is the most lethal malignant primary brain tumor; nevertheless, there remains a lack of accurate prognostic markers and drug targets. In this study, we analyzed 117 primary glioblastoma patients’ data that contained SNP, DNA copy, DNA methylation, mRNA expression, and clinical information. After the quality of control examination, we conducted the single nucleotide polymorphism (SNP) analysis, copy number variation (CNV) analysis, and infiltrated immune cells estimate. And moreover, by using the cluster of cluster analysis (CoCA) methods, we finally divided these GBM patients into two novel subtypes, HX-1 (Cluster 1) and HX-2 (Cluster 2), which could be co-characterized by 3 methylation variable positions [cg16957313(DUSP1), cg17783509(PHOX2B), cg23432345(HOXA7)] and 15 (PCDH1, CYP27B1, LPIN3, GPR32, BCL6, OR4Q3, MAGI3, SKIV2L, PCSK5, AKAP12, UBE3B, MAP4, TP53BP1, F5, RHOBTB1) gene mutations pattern. Compared to HX-1 subtype, the HX-2 subtype was identified with higher gene co-occurring events, tumor mutation burden (TBM), and poor median overall survival [231.5 days (HX-2) vs. 445 days (HX-1),P-value = 0.00053]. We believe that HX-1 and HX-2 subtypes may make sense as the potential prognostic biomarkers for patients with glioblastoma.
Th17-Immune Response in Patients With Membranous Nephropathy Is Associated With Thrombosis and Relapses
Authors: Cremoni Marion , Brglez Vesna , Perez Sandra , Decoupigny Fabrice , Zorzi Kévin , Andreani Marine , Gérard Alexandre , Boyer-Suavet Sonia , Ruetsch Caroline , Benzaken Sylvia , Esnault Vincent , Seitz-Polski Barbara
Published: November 2020

Membranous nephropathy (MN) is a rare autoimmune kidney disease. Most autoimmune diseases are associated with a pro-inflammatory Th17-immune response, but little is known about immune dysregulation in MN. In China, MN was associated with exposure to fine air particulate matter (PM2.5) that could act as a danger signal and redirect immune response toward the Th2 or Th17 pathway. We aimed to analyze the cytokine profile of MN patients and to study the possible environmental factors involved in this immune reorientation, as well as the consequences on the prognosis of the disease. In this prospective study, 59 MN patients filled a comprehensive lifestyle questionnaire. Peripheral blood cells from MN patients were stimulated in vitro to measure the cytokines produced in supernatant. Cytokine profiles of MN patients were compared to 28 healthy donors and analyzed regarding individual PM2.5 exposure. Compared to healthy donors, MN patients had higher serum levels of Th17 and Th2 cytokines IL-17A (62 pg/ml [IQR, 16–160] versus 31 [IQR, 13–51], P=0.035), IL-6 (66767 pg/ml [IQR, 36860–120978] versus 27979 [IQR, 18672–51499], P=0.001), and IL-4 (12 pg/ml [IQR, 0–33] versus 0 pg/ml [IQR, 0–0], P=0.0003), respectively, as well as a deficiency of Th1 and regulatory T cell cytokines IFN-γ (5320 pg/ml [IQR, 501–14325] versus 18037 [IQR, 4889–31329], P=0.0005) and IL-10 (778 pg/ml [IQR, 340–1247] versus 1102 [IQR, 737–1652], P=0.04), respectively. MN patients with high IL-17A levels lived in areas highly exposed to PM2.5: 51 μg/m3 versus 31 μg/m3 for patients with low IL-17A levels (P=0.002) while the World Health Organization recommends an exposition below 10 μg/m3. MN patients with Th17-mediated inflammation had more venous thromboembolic events (P=0.03) and relapsed more often (P=0.0006). Rituximab treatment induced Th1 and regulatory T cell cytokines but did not impact Th17 cytokines. MN patients with Th17-mediated inflammation which appears to be related to an urban environment have worse prognosis. Alternative strategies targeting dysregulated cytokine balance could be considered for these patients at high risk of relapse.
Emerging Neuroblastoma 3D In Vitro Models for Pre-Clinical Assessments
Authors: Corallo Diana , Frabetti Stella , Candini Olivia , Gregianin Elisa , Dominici Massimo , Fischer Horst , Aveic Sanja
Published: November 2020

The potential of tumor three-dimensional (3D) in vitro models for the validation of existing or novel anti-cancer therapies has been largely recognized. During the last decade, diverse in vitro 3D cell systems have been proposed as a bridging link between two-dimensional (2D) cell cultures and in vivo animal models, both considered gold standards in pre-clinical settings. The latest awareness about the power of tailored therapies and cell-based therapies in eradicating tumor cells raises the need for versatile 3D cell culture systems through which we might rapidly understand the specificity of promising anti-cancer approaches. Yet, a faithful reproduction of the complex tumor microenvironment is demanding as it implies a suitable organization of several cell types and extracellular matrix components. The proposed 3D tumor models discussed here are expected to offer the required structural complexity while also assuring cost-effectiveness during pre-selection of the most promising therapies. As neuroblastoma is an extremely heterogenous extracranial solid tumor, translation from 2D cultures into innovative 3D in vitro systems is particularly challenging. In recent years, the number of 3D in vitro models mimicking native neuroblastoma tumors has been rapidly increasing. However, in vitro platforms that efficiently sustain patient-derived tumor cell growth, thus allowing comprehensive drug discovery studies on tailored therapies, are still lacking. In this review, the latest neuroblastoma 3D in vitro models are presented and their applicability for a more accurate prediction of therapy outcomes is discussed.
Modeling Coral Bleaching Mitigation Potential of Water Vertical Translocation – An Analogue to Geoengineered Artificial Upwelling
Authors: Feng Ellias Y. , Sawall Yvonne , Wall Marlene , Lebrato Mario , Fu Yao
Published: November 2020

Artificial upwelling (AU) is a novel geoengineering technology that brings seawater from the deep ocean to the surface. Within the context of global warming, AU techniques are proposed to reduce sea surface temperature at times of thermal stress around coral reefs. A computationally fast but coarse 3D Earth System model (3.6° longitude × 1.8° latitude) was used to investigate the environmental impacts of hypothetically implemented AU strategies in the Great Barrier Reef, South China Sea, and Hawaiian regions. While omitting the discussion on sub-grid hydrology, we simulated in our model a water translocation from either 130 or 550 m depth to sea surface at rates of 1 or 50 m3 s–1 as analogs to AU implementation. Under the Representative Concentration Pathway 8.5 emissions scenario from year 2020 on, the model predicted a prevention of coral bleaching until the year 2099 when AU was implemented, except under the least intense AU scenario (water from 130 m depth at 1 m3 s–1). Yet, intense AU implementation (water from 550 m depth at 50 m3 s–1) will likely have adverse effects on coral reefs by overcooling the surface water, altering salinity, decreasing calcium carbonate saturation, and considerably increasing nutrient levels. Our result suggests that if we utilize AU for mitigating coral bleaching during heat stress, AU implementation needs to be carefully designed with respect to AU’s location, depth, intensity and duration so that undesirable environmental effects are minimized. Following a proper installation and management procedure, however, AU has the potential to decelerate destructive bleaching events and buy corals more time to adjust to climate change.
Polymer Adhesin Domains in Gram-Positive Cell Surface Proteins
Authors: Järvå Michael A. , Hirt Helmut , Dunny Gary M. , Berntsson Ronnie P.-A.
Published: November 2020

Surface proteins in Gram-positive bacteria are often involved in biofilm formation, host-cell interactions, and surface attachment. Here we review a protein module found in surface proteins that are often encoded on various mobile genetic elements like conjugative plasmids. This module binds to different types of polymers like DNA, lipoteichoic acid and glucans, and is here termedpolymer adhesin domain.We analyze all proteins that contain a polymer adhesin domain and classify the proteins into distinct classes based on phylogenetic and protein domain analysis. Protein function and ligand binding show class specificity, information that will be useful in determining the function of the large number of so far uncharacterized proteins containing a polymer adhesin domain.
Exopolysaccharide Producing Bifidobacterium animalis subsp. lactis Strains Modify the Intestinal Microbiota and the Plasmatic Cytokine Levels of BALB/c Mice According to the Type of Polymer Synthesized
Authors: Sabater Carlos , Molinero-García Natalia , Castro-Bravo Nuria , Diez-Echave Patricia , Hidalgo-García Laura , Delgado Susana , Sánchez Borja , Gálvez Julio , Margolles Abelardo , Ruas-Madiedo Patricia
Published: November 2020

Bacteria-host interactions are mediated by different microbial associated molecular patterns which are most often surface structures such as, among others, exopolysaccharides (EPSs). In this work, the capability of two isogenic EPS-producing Bifidobacterium animalis subsp. lactis strains to modulate the gut microbiota of healthy mice, was assessed. Each strain produces a different type of polymer; the ropy strain S89L synthesized a rhamnose-rich, high-molecular weight EPS in highest abundance than the non-ropy DMS10140 one. BALB/c mice were orally fed for 10 days with milk-bifidobacterial suspensions and followed afterward for 7 post-intervention days (wash-out period). The colonic content of mice was collected in several sampling points to perform a metataxonomic analysis. In addition, the influence of specific microbial clades, apparently stimulated by the ropy and non-ropy strains, on mouse plasmatic cytokine levels was investigated through hierarchical association testing. Analysis of 16S rRNA gene sequences showed that the abundance of Firmicutes phylum significantly increased 7 days after cessing the treatment with both strains. The relative abundance of Alloprevotella genus also rose, but after shorter post-treatment times (3 days for both DMS10140 and S89L strains). Some bacterial clades were specifically modulated by one or another strain. As such, the non-ropy DMS10140 strain exerted a significant influence on Intestinomonas genus, which increased after 4 post-administration days. On the other hand, feeding with the ropy strain S89L led to an increase in sequences of Faecalibaculum genus at 4 post-treatment days, while the abundance of Erysipelotrichaceae and Lactobacillaceae families increased for prolonged times. Association testing revealed that several lactobacilli and bifidobacterial significantly stimulated by ropy S89L strain were positively associated with the levels of certain cytokines, including IL-5 and IL-27. These results highlight relevant changes in mice gut microbiota produced after administration of the ropy S89L strain that were associated to a potential immune modulation effect.
Structural Features of Heparin and Its Interactions With Cellular Prion Protein Measured by Surface Plasmon Resonance
Authors: Kim So Young , Zhang Fuming , Harris David A. , Linhardt Robert J.
Published: November 2020

Self-propagating form of the prion protein (PrPSc) causes many neurodegenerative diseases, such as Creutzfeldt-Jakob disease (CJD) and Gerstmann-Straussler-Scheinker syndrome (GSS). Heparin is a highly sulfated linear glycosaminoglycan (GAG) and is composed of alternating D-glucosamine and L-iduronic acid or D-glucuronic acid sugar residues. The interactions of heparin with various proteins in a domain-specific or charged-dependent manner provide key roles on many physiological and pathological processes. While GAG-PrP interactions had been previously reported, the specific glycan structures that facilitate interactions with different regions of PrP and their binding kinetics have not been systematically investigated. In this study, we performed direct binding surface plasmon resonance (SPR) assay to characterize the kinetics of heparin binding to four recombinant murine PrP constructs including full length (M23–230), a deletion mutant lacking the four histidine-containing octapeptide repeats (M23–230 Δ59–90), the isolated N-terminal domain (M23–109), and the isolated C-terminal domain (M90–230). Additionally, we found the specific structural determinants required for GAG binding to the four PrP constructs with chemically defined derivatives of heparin and other GAGs by an SPR competition assay. Our findings may be instrumental in developing designer GAGs for specific targets within the PrP to fine-tune biological and pathophysiological activities of PrP.
Impacts of Environmental Factors on Pasting Properties of Cassava Flour Mediated by Its Macronutrients
Authors: Zhang Yayuan , Nie Lei , Sun Jian , Hong Yan , Yan Huabing , Li Mingjuan , You Xiangrong , Zhu Ling , Fang Fang
Published: November 2020

The impacts of environmental conditions on pasting and physicochemical properties were investigated using flour samples of the same cassava cultivar grown in seven different locations. Significant location differences in essential component (except for fiber) content of cassava flour were observed. Cassava flour showed obviously separated traits in the principal component analysis (PCA) of near-infrared spectra (NIR) according to geographical origins. The environmental effects were significant in the pasting properties of cassava flours. Sufficient precipitation and suitable low temperature promoted accumulation of starch in cassava, resulting in the high peak viscosity values of cassava flour. Pasting temperatures of cassava flour had a significant direct correlation with growth temperature and were negatively correlated with altitude. Precipitation from August to October showed a stronger direct correlation with trough and final viscosity. The results of this study indicated the possibility of predicting and controlling cassava flour quality and pasting properties according to the environmental conditions.
Chemoradiotherapy Is Inferior to Chemotherapy Alone in Adjuvant Setting for Signet Ring Cell Containing Gastric Cancer
Authors: Zhu Yue-Ting , Chen Xin-Zu , Chen Ye , Zhou Yu-Wen , Tang Lian-Sha , Luo De-Yun , Li Qiu , Qiu Meng , Wang Xin , Cao Dan , Yang Yu , Shen Ya-Li , Li Zhi-Ping , Bi Feng , Liu Ji-Yan , Gou Hong-Feng
Published: November 2020

Background

Signet ring cell containing gastric cancer (SRCGC) is a rare subtype of gastric cancer, and its adjuvant therapy is based on general gastric cancer. However, the effectiveness of radiotherapy for those SRCGC patients remains unknown.

Purpose

The purpose of the study was to analyze whether the addition of radiotherapy to adjuvant chemotherapy (CT) can benefit survival in resected SRCGC patients.

Methods

Patients with SRCGC, who underwent D2 gastrectomy followed by adjuvant chemotherapy or chemoradiotherapy (CRT), were retrospectively collected. According to the proportion of signet ring cells, patients were histologically classified as pure SRCGC (pSRCGC) containing 100% of signet ring cells, mixed SRCGC (mSRCGC) containing >50% of signet ring cells, and contaminated SRCGC (cSRCGC) containing <50% of signet ring cells. Among the 272 patients, 156 were treated by CT alone and 116 by CRT. The primary endpoint was 3-year overall survival rate (3-year OS rate).

Results

With a median follow-up of 80.5 months, the 3-year OS rate was significantly higher in the CT group (70.5% vs. 58.6%, HR = 0.633, P = 0.017) compared with CRT group. Three independent characteristics were predictive of a poor overall survival: CRT treatment (P = 0.019), tumor size ≥5 cm (P < 0.001), and the presence of vessel invasion (P = 0.009). Subgroup analyses showed CRT significantly impaired prognosis in SRCGC patients in the cSRCGC subset, as well as lesions located in lower-middle sites, subtotal gastrectomy, male, <60 year, and no vessel invasion. Peritoneal was the most common recurrence site in SRCGC patients. The adverse events leukopenia and neutropenia were more common in the CRT group (P = 0.007).

Conclusions

Adjuvant chemoradiotherapy was associated with poor survival compared with adjuvant chemotherapy in SRCGC patients with D2 gastrectomy.