bluuebunny/crossref_metadata_2025 huggingface dataset:
Percepção matemática na educação infantil: Contribuições para a prática educativa
Authors: Castro Joelma Fátima , Santos Edilson de Araújo dos , Lacanallo Arrais Luciana Figueiredo
Published: January 2023

Neste artigo expomos uma investigação resultante de uma pesquisa de Iniciação Científica (IC) que teve como objetivo discutir o desenvolvimento da percepção matemática na educação infantil. Mediante uma pesquisa de caráter bibliográfico, utilizamos os pressupostos da Teoria Histórico-Cultural para destacar a necessidade do trabalho pedagógico organizado desde o ingresso das crianças na educação infantil, pois é por esse trabalho que os sujeitos poderão se apropriar das bases conceituais, alcançando as condições para seu pleno desenvolvimento psíquico. O artigo está divido em três momentos. Iniciamos caracterizando as funções mentais envolvidas no processo de desenvolvimento humano e por que a percepção precisa ser considerada no trabalho educativo. Na sequência, definimos o que é percepção matemática. Por fim, apresentamos como, por meio de ações de ensino intencionalmente planejadas, os professores podem organizar o ensino para o desenvolvimento da percepção Matemática em crianças da educação infantil. Esperamos que esta pesquisa contribua para o ensino de matemática na educação infantil, na direção de repensar e reorganizar as ações de ensino nessa etapa.
Inovação metodológica da prática pedagógica: um olhar para as disciplinas curriculares de um curso de licenciatura em ciências biológicas (UTFPR)
Authors: Schneider Eduarda Maria , Machado Jeniffer Sabrina , Nunes Silvana Aguero
Published: January 2023

Esta pesquisa objetiva analisar os planos de ensino das disciplinas ministradas em 2019 em um curso de Licenciatura em Ciências Biológicas da Universidade Tecnológica Federal do Paraná (UTFPR), a fim de verificar a proposta de metodologias e modalidades ativas nos procedimentos de ensino das disciplinas, tanto nas aulas teóricas, práticas quanto na complementação de carga horária (CCH). A metodologia fundamenta-se em uma abordagem qualitativa com finalidade exploratória mediante pesquisa bibliográfica e documental dos planos de ensino de 54 disciplinas obrigatórias que foram reunidas de acordo com as áreas de conhecimento da Capes para Ciências Biológicas. Constatamos que foi proposta uma boa variedade de metodologias e modalidades didáticas nos planos de ensino, já com a inclusão de metodologias ativas; contudo verificamos que, na maioria das disciplinas, o ensino tradicional e dialógico ainda aparece de forma prioritária. Nesse sentido, para atender às necessidades que vêm surgindo atualmente, principalmente após a pandemia da Covid-19, o currículo do curso precisa ser atualizado e adequado, com a intenção de valorizar a formação do corpo docente para que, assim, possam aprimorar seus métodos e metodologias de ensino de modo a atender às necessidades atuais da formação docente da educação básica.
The Impact of Prenatal Diagnosis and Treatment on Early Neonatal Problems
Authors: Sinaci Selcan , Kadioglu Simsek Gulsum , Sakcak Bedri , Ozgurluk Izzet , Canpolat Fuat Emre , Sahin Dilek
Published: January 2023

OBJECTIVE: Obstetric problems have detrimental effects on both pregnancy and newborn. We aimed to examine the relationship between pregnancy complications and newborn morbidities.
STUDY DESIGN: The newborns admitted to the neonatal intensive care unit between September 2019 and August 2022 were included in this retrospective cross-sectional study. Antenatal morbidities and neonatal complications including low APGAR-score (<5), bronchopulmonary dysplasia, early neonatal sepsis, and early neonatal death were evaluated. Pearson-χ2 cross-tabs were used to compare two variables. A p-value <0.05 was determined statistically significant.
RESULTS: Among the 686 infants analyzed, 94 (13.7%) had early neonatal death, 264 (38.5%) had bronchopulmonary dysplasia, 259 (37.8%) had early neonatal sepsis, and 424 (61.9%) had feeding intolerance. Early neonatal sepsis was observed significantly higher in newborns whose mothers had urinary tract infection in the last two weeks (p<0.001), preterm premature rupture of membranes (p=0.006), or clinical chorioamnionitis (p<0.001). Early neonatal death was found significantly higher in pregnancies with preeclampsia (p<0.001), preterm premature rupture of membranes (p<0.001), clinical chorioamnionitis (p<0.001), or small-for-gestational-age (p<0.001). Preeclampsia and magnesium neuroprophylaxis were found significantly higher in neonates with feeding intolerance (p<0.001). Backward: LR logistic regression analysis based on early neonatal sepsis risk revealed that birth week, preterm premature rupture of membranes, clinical chorioamnionitis, small-for-gestational-age, and urinary tract infection were significant parameters affecting the risk of early neonatal sepsis (p<0.05).
CONCLUSION: Preventing premature births and cautious management of pregnancy complications may be helpful in reducing adverse neonatal outcomes. Early detection of high-risk pregnancies and transfer to a tertiary center may be helpful to improve neonatal outcomes.
Osteonecrosis maxilar por electrocirugía enseñanzas de un caso clínico
Authors: Riche-Jimeno Yeisson , Campuzano-Gracián Heiner , Díaz-Caballero Antonio
Published: January 2023

La utilización de electrocauterios como elementos de apoyo en los procesos de hemostasia ha sido ampliamente reportada y hace parte de las herramientas en procedimientos quirúrgicos en diferentes áreas médicas, aunque su uso en procedimientos en cavidad oral sea menor. Actualmente su aplicación ha venido aumentando y se encuentra involucrado en diversos procedimientos odontológicos. Al analizar la literatura, se encuentra muy poca información en cuanto a las complicaciones asociadas al uso del electrobisturí, las cuales son poco frecuentes o no reportadas. Se presenta un reporte de caso sobre Osteonecrosis inducida por electrobisturí en zona proximal de incisivos centrales, posterior a frenillectomía vestibular en maxilar superior. El proceso de sobreexposición del hueso maxilar a la acción de puntas de electrocirugía puede desencadenar Osteonecrosis. Los secuestros óseos generados crean posteriores defectos periodontales tras su remoción. Los movimientos ortodónticos acompañados de recontorneo de puntos Zenith e injertos de tejido conectivo pueden ser una alternativa en el manejo estético de la complicación.
Eficacia anestésica del bloqueo del nervio dentario inferior en molares con pulpitis: una revisión sistemática tipo paraguas
Authors: Urueta-Álvarez Nahomy Victoria , Díaz-Caballero Antonio José , Martínez-Martínez Adel Alfonso
Published: January 2023

Obtener una alta eficacia anestésica en molares mandibulares con pulpitis ha sido un proceso complejo, dado la combinación de múltiples factores, entre ellos el ph del medio y la técnica a utilizar. El objetivo de la presente revisión fue comparar la evidencia disponible sobre la eficacia anestésica del bloqueo del nervio alveolar inferior en molares. Se realizó búsqueda sistemática de la literatura empleando los buscadores de información: PubMed, Science-Direct, Scopus, Ebscohost / Dentistry & oral sciences source y ClinicalKey. Los estudios fueron seleccionados a través de análisis y la lista de chequeo del formato PRISMA, fueron escogidas aquellas revisiones sistemáticas cuyo objetivo fue evaluar la efectividad del bloqueo del nervio alveolar inferior en molares con pulpitis, siete estudios cumplieron con los criterios de inclusión. La extracción de datos se realizó mediante la lectura crítica de las revisiones sistemáticas seleccionadas previamente, los resultados de esta revisión sistemática tipo paraguas muestran efectos positivos en cuanto a eficacia anestésica al realizar el bloqueo del nervio alveolar inferior en casos de pulpitis irreversible hace referencia, la efectividad entre estudios varía dependiendo de los anestésicos utilizados. Concluyendo que muchos factores pueden afectar la concordancia de los resultados, aunque el uso de IANB en casos de pulpitis en molares muestra buenos resultados, aún no alcanza una tasa de éxito anestésico mayor al 80%, sin importar el uso de la articaína que fue el anestésico con mejores resultados en la mayoría de revisiones.
Women, technology, and water: creating new waterscapes and contesting cultural norms
Authors: Sarkar Anindita
Published: January 2023

With the analysis of primary data, the paper looks at how the adoption of new modes of water technologies and subsequent water commercialization has created new waterscapes. Water commercialization is changing cultural norms associated with water collection in the drought-prone villages of western Rajasthan. The newly introduced water tankers have selectively benefited the upper caste rich households who now buy water from the tankers at doorsteps. The majority of the poor and low caste households remain dependent on common water sources that are drying up due to neglect with the advent of water commercialization. Women from low caste poor households have to walk for long distances to fetch water in addition to doing physical labor to support family income. Though domestic water use remains a domain of woman's working space, men from high caste rich households have started fetching and storing water as it entails cash transactions.
El Ícono frente al arte: sus particularidades, diferencias e implicancias
Authors: Hidalgo Leandro
Published: January 2023

En este artículo se analiza la dimensión del ícono,entendido como manifestación de Dios hacia el hombre,y cómo a través de estemediosecrea un encuentro.Estarelación se estudia en elámbito del arte, específicamente el que está inspirado en motivos religiosos; desde aquí se hace un análisis de las particularidades del ícono frente a éste.Para esto, se rastreará el origen del ícono y las características esenciales que lo han constituido como la imagen de culto por excelencia,frente a todo el arte religioso que se ha ido produciendo a lo largo de la historia del arte. Para ello, se busca vislumbrar cómo los grandes artistas clásicos han cambiado el enfoque de lo plasmado y han puesto la mirada en la belleza estética, más que en el trasfondo de la imagen realizada; de esta manera, es clave el rol que juega el artista en ambos tipos de producción.
La tragedia Ática a la luz de la filosofía de Nietzsche
Authors: Sandoval Castro Iván
Published: January 2023

Nietzsche da origen a la duplicidad de los términos que denominó como lo apolíneoy lo dionisíaco, realizando a su vez, un viaje de profundo contenido filosófico y filológico en su obra de juventud que lleva comotítulo:El nacimiento de la tragedia. El presente trabajotiene por finalidad reflexionar a través de la distincióny contraposiciónentre estos dos conceptos llevada a cabo por el joven Nietzscheen dicha obra, así como también, enindagar en el desarrollo de latragedia griega como el suceso histórico en el que tanto lo apolíneo como lo dionisíaco coexisten armónicamente gracias aun actomilagrosode la voluntad helénica, intentandoa su vez,responder a la pregunta sobre el origen de la tragedia ática. Luego se hará énfasis en la posterior decadencia de la tragedia a través de autores de la talla deEurípides,yen cómo repercute en este sucesolafigura de Sócrates, respondiendo asífinalmente a la pregunta por la decadenciade la tragedia ática.
The Association of LDH Expression and Delayed Cerebral Ischemia in
Patients with Aneurysmal Subarachnoid Hemorrhage: Possible Involvement
of Cerebral Blood Perfusion
Authors: Sun Xiaochuan , Wu Yue , Wang Yingwen , Tang Shuang , Yang Xiaomin , Cao Yunchuan , Li Xiaoguo , Xu Rui , Yan Jin , Guo Zongduo
Published: January 2023

Background and Purpose:

Several pieces of evidence suggest that serum lactate hydrogenase
(LDH) level is associated with the pathological process of delayed cerebral ischemia (DCI)
after aneurysmal subarachnoid hemorrhage (aSAH). This research aimed to investigate the associations
of serum LDH level with the occurrence of DCI in aSAH patients.

Methods:

A total of 122 patients diagnosed with aSAH within 72h of onset were retrospectively
enrolled. The serum levels of LDH between 7:00-8:00 am on day 1, day 3 and day 7, patients’ demographics,
and clinical features were collected. Computed tomography perfusion was performed
within 7 days after aSAH. The occurrence of DCI was recorded during the hospitalization.

Results:

Among all the enrolled patients, 43 (35.2%) developed DCI during hospitalization. Patients
occurred DCI were always accompanied by more serious clinical features and found with higher
serum LDH levels. LDH levels on day 3 and day 7 after onset were independently associated with
the occurrence of DCI and showed high predictive value according to the receiver operating characteristic
(ROC) curve. Moreover, there was a strong correlation between LDH and mean cerebral
blood flow, transit time, and mean time to peak.

Conclusions:

Serum LDH level on day 3 and day 7 may be a valuable, convenient, and rapid predictive
indicator for the occurrence of DCI in aSAH patients.
Hetero Cyclic Compounds in the Treatment of Triple-Negative Breast Cancer
Authors: Rath Santosh Kumar , Mandal Sudip Kumar , Das Agnidipta , Bose Anindya , Dwibedi Vagish , Ganguly Paramita , Sarkar Sipra , Prakash Ranjana , Dey Biplab Kumar , Mandal Sanjeet
Published: January 2023

Triple-negative breast cancer (TNBC) holds just about 15% of all breast tumours and subtypes of breast cancer with distinct characteristics of negative expressions for the progesterone receptor, estrogen receptor, and human epidermal growth factor receptor 2. Unfortunately, treatment options for TNBCs are minimal. Most currently available therapies proved inefficient in holding back this aggressive natural treatment of TNBC, in most cases calling for an immediate need for more effective and safer anti-TNBC agents. Based on research reported in recent years, this review presents the report's overview of anti-TNBC compounds and their efficacy, being classified according to the structures. Breast Cancer type 1 and type 2 genes (BRCA1/2) mutations are associated with TNBC. Poly (ADP-Ribose) Polymerases (PARPs) are a family of enzymes involved in numerous cellular processes, including DNA repair. PARP-1 inhibition is involved in the loss of DNA repair via BRCA-dependent mechanisms. PARP-1 inhibitors like Olaparib, Rucaparib, Niraparib, and Talazoparib have proved as promising therapeutic medications as monotherapy and in combination with cytotoxic therapy or radiotherapy in various types of cancers. This review is focused on presenting the status of therapeutics against TNBC. The critical spotlight of this review is to encapsulate the versatility and notable success of heterocyclic pharmacophores-based molecules in treating TNBC.
Avaliar para aprender em ciências experimentais
Authors: Sousa Margarida Teixeira , Santos Leonor
Published: January 2023

Este artigo reporta um estudo que tem por objetivo compreender como uma professora portuguesa do 3.º ciclo do ensino básico desenvolve práticas avaliativas em atividades investigativas de Ciências Naturais para orientar e apoiar a aprendizagem dos seus alunos. Optámos por uma abordagem metodológica interpretativa, com design de estudo de caso. A professora Eva é o caso deste estudo. Recorreu-se à entrevista, observação de aulas e pesquisa documental, para recolha de dados. A metodologia usada foi a de análise de conteúdo. Os resultados obtidos evidenciam que as práticas de avaliação formativa da professora Eva refletem a importância de o aluno aprender acerca da ciência e a pensar a ciência, numa abordagem Inquiry Based Science Education. Enquanto instrumentos ao serviço do ensino e da aprendizagem, estas práticas de avaliação formativa são de natureza formal e informal. Para obter evidências que melhorem o desenvolvimento de competências científicas, a professora Eva usa como estratégias o feedback oral e escrito, a explicitação dos objetivos de aprendizagem e de critérios de avaliação e o incentivo ao trabalho colaborativo por parte dos alunos. A estas estratégias junta-se a seleção criteriosa de tarefas de natureza investigativa de inquérito estruturado, guiado e aberto. As implicações para contextos de formação de professores mostram-se orientadas por um diálogo de práticas avaliativas.
Ejes y perfiles de implicación familiar en la etapa de Educación Infantil: El caso de “La Escuelita”
Authors: Amber Diana , Morales Valero Manuel
Published: January 2023

En educación infantil, familia y escuela deben trabajar de forma coordinada para promover el desarrollo integral de la infancia. Son muchas las propuestas educativas que, conscientes de ello, abren sus puertas a familias y comunidad. El proyecto educativo alternativo que centra este trabajo (denominado afectuosamente “La Escuelita”) es referente local de implicación familiar, por ello, a partir de su estudio, esta investigación se propone comprender las dinámicas familiares para definir los ejes y perfiles de participación de la familia presentes en este proyecto. Se utiliza el estudio de caso único de diseño incrustado como opción metodológica, incluyendo 24 unidades de análisis, y la entrevista en profundidad como instrumento de recogida de información. Los resultados desvelan dos ejes de implicación familiar: participación activa e ideología pedagógica, que dibujan cuatro perfiles diferenciados presentes en las familias (delegador, colaborador, sensibilizado y comprometido). Se concluye que, aunque los cuatro perfiles coexisten en “La Escuelita”, se aprecia un predominio de los más implicados, frente a aquellos que delegan la educación al centro escolar. El aporte principal de este trabajo radica en el modelo de ejes de implicación familiar diseñado, útil como marco de referencia para posteriores análisis en otros contextos educativos.
Se utiliza el estudio de caso único de diseño incrustado como opción metodológica, incluyendo 24 unidades de análisis, y la entrevista en profundidad como instrumento de recogida de información.
Los resultados desvelan dos ejes de implicación familiar: participación activa e ideología pedagógica, que dibujan cuatro perfiles diferenciados presentes en las familias (delegador, colaborador, sensibilizado y comprometido). Se concluye que, aunque los cuatro perfiles coexisten en el centro, se aprecia un predominio de los más implicados, frente a aquellos que delegan la educación al centro escolar. La principal contribución de este trabajo radica en el modelo de ejes de implicación familiar diseñado, útil como marco de referencia para posteriores análisis en otros contextos educativos.
Processos de participação de estudantes do ensino técnico integrado: Estudo da realidade de uma instituição de ensino no Estado do Amazonas, Brasil
Authors: Nádia Falcão Nádia Maciel Falcão , Martha Victor Martha Lima Reis Victor , Ada Vasconcelos Ada Raquel da Fonseca Vasconcelos
Published: January 2023

O estudo analisa limites e possibilidades da participação de estudantes do ensino técnico integrado – modalidade de oferta do ensino médio articulado à educação profissional – no âmbito dos órgãos colegiados implantados em uma das unidades de ensino de uma instituição pertencente à Rede Federal de Educação Profissional, Científica e Tecnológica (REPECT), situada na Cidade de Manaus, no Estado do Amazonas, Brasil. A metodologia, de abordagem qualitativa, contemplou: revisão da literatura de referência sobre a temática; análise da legislação que trata do princípio da gestão democrática do ensino público no Brasil e da Rede Federal de Educação Profissional, Científica e Tecnológica; e análise de documentos da instituição pesquisada, tais como: Estatuto e Regimento Geral, Estatuto do Código Eleitoral do Conselho Superior, Organização Didático-Pedagógica, Plano de Desenvolvimento Institucional (PDI) e Regulamento do Conselho de Classe. Contemplou, ainda, a aplicação de entrevista semiestruturada a estudantes e profissionais que atuavam como representantes de seus segmentos em instâncias colegiadas da instituição. Os resultados evidenciam que a instituição possui normas para implantação de órgãos colegiados, todavia, verificam-se entraves à efetivação destes canais de participação. Observam-se processos embrionários de participação estudantil, sendo o Conselho de Classe o único colegiado com assento para estudantes já implantado naquela unidade da instituição. Na dinâmica de funcionamento deste órgão, a participação esbarra na cultura centralizadora, cabendo aos estudantes e demais sujeitos da comunidade escolar o engajamento para construção de práticas e pautas alinhadas ao princípio da gestão democrática.
IMPLEMENTASI ALGORITMA GENETIKA DALAM PENENTUAN RUTE OPTIMAL UNTUK KURIR KANTOR POS BERBASIS WEB (STUDI KASUS: KANTOR POS WATES)
Authors: Ihsani Imam , Pramuntadi Andri , Gutama Deden Hardan , Wijaya Dhina Puspasari
Published: January 2023

Ekspedisi menjadi media penting dalam proses pengiriman barang
 oleh banyak orang maupun berbagai instansi. Terlebih situasi pandemi
 yang membuat jasa ekspedisi kian ikut meningkat pula. Penulis
 melakukan penelitian pada Kantor Pos Wates yang berada di bawah
 perusahaan Pos Indonesia yang justru peringkatnya jauh berada di
 bawah ekspedisi swasta lainnya. Faktor penurunan ini kemungkinan
 karena permasalahan pengiriman barang oleh kurir yang disebut
 dengan TSP (Traveling Salesman Problem) dimana kurir kesulitan
 menentukan rute terpendek dalam pengantaran paket kepada
 pelanggan di setiap lokasi pengirimannya mulai dari titik awal sampai
 kembali ke titik awal lagi. Solusi permasalahan ini adalah penerapan
 algoritma genetika untuk pengiriman barang dengan hasil keluaran
 aplikasi berbasis web sehingga kurir dapat mengirimkan barang lebih
 efektif untuk menghemat jarak, waktu dan biaya. Algoritma genetika
 adalah algoritma metaheuristik sehingga mampu menyelesaikan
 masalah sampai optimal. Tahapan algoritma genetika meliputi
 pembangkitan populasi, individu, kromosom, penentuan gen,
 penentuan fitness, seleksi untuk mendapatkan parents, crossover
 untuk menghasilkan offspring, mutasi, sampai menghasilkan solusi.
 Dalam pembuatan aplikasi digunakan API pada Google Maps untuk
 menampilkan titik koordinat lokasi yang didefinisikan sebagai gen.
 Perancangan aplikasi ini menggunakan metode Waterfall agar proses
 pembuatannya dilakukan secara urut dan terstruktur. Bahasa
 pemrograman yang digunakan dalam perancangan ini adalah PHP
 untuk menanamkan algoritma genetika pada web-nya. Adapula hasil
 dari penelitian ini adalah aplikasi web penentu rekomendasi rute yang
 di-generate berdasarkan jarak terdekat yang dapat dilalui kurir.
PENERAPAN METODE GAP PADA SISTEM PENDUKUNG KEPUTUSAN PENENTUAN BIBIT PADI TERBAIK
Authors: Ricky Ichsan Muhammad Adithya , Wulandari Irma Rofni , Astuti Yuli , Widayani Wiwi
Published: January 2023

Pemilihan bibit padi merupakan langkah awal dari budidaya tanaman padi. Pemilihan bibit perlu diperhatikan untuk mendapatkan hasil panen yang baik. Petani di Desa Soropadan sering mengalami kesulitan dalam menentukan bibit padi yang akan digunakan. Sistem pendukung keputusan dapat menjadi salah satu solusi untuk permasalahan pemilihan bibit padi. Jenis bibit padi dapat dipilih berdasarkan aspek dan kriteria tertentu. Kriteria yang digunakan yaitu tinggi tanaman, kerontokan, harga bibit, umur tanaman, bentuk tanaman, bentuk gabah, kadar amilosa, dan kerebahan. Metode GAP merupakan salah satu metode pengambilan keputusan dengan mencari gap antar kriteria. Tujuan dari penelitian ini yaitu untuk merancang suatu sistem penunjang keputusan dengan menerapkan metode GAP sehingga dapat membantu memberikan rekomendasi bibit padi unggul. Hasil dari penelitian yaitu Metode GAP berhasil diimplementasikan ke dalam bentuk sistem penunjang keputusan berbasis website dan menampilkan perankingan bibit padi serta memberikan rekomendasi bibit padi terbaik. Blackbox testing digunakan untuk menguji sistem dan mendapatkan hasil sistem berjalan sesuai dengan perancangan.
Disruptions in the Cystic Fibrosis Community’s Experiences and Concerns During the COVID-19 Pandemic: Topic Modeling and Time Series Analysis of Reddit Comments (Preprint)
Authors: Yao Lean Franzl , Ferawati Kiki , Liew Kongmeng , Wakamiya Shoko , Aramaki Eiji
Published: January 2023

BACKGROUND

The COVID-19 pandemic disrupted the needs and concerns of the cystic fibrosis community. Patients with cystic fibrosis were particularly vulnerable during the pandemic due to overlapping symptoms in addition to the challenges patients with rare diseases face, such as the need for constant medical aid and limited information regarding their disease or treatments. Even before the pandemic, patients vocalized these concerns on social media platforms like Reddit and formed communities and networks to share insight and information. This data can be used as a quick and efficient source of information about the experiences and concerns of patients with cystic fibrosis in contrast to traditional survey- or clinical-based methods.

OBJECTIVE

This study applies topic modeling and time series analysis to identify the disruption caused by the COVID-19 pandemic and its impact on the cystic fibrosis community’s experiences and concerns. This study illustrates the utility of social media data in gaining insight into the experiences and concerns of patients with rare diseases.

METHODS

We collected comments from the subreddit r/CysticFibrosis to represent the experiences and concerns of the cystic fibrosis community. The comments were preprocessed before being used to train the BERTopic model to assign each comment to a topic. The number of comments and active users for each data set was aggregated monthly per topic and then fitted with an autoregressive integrated moving average (ARIMA) model to study the trends in activity. To verify the disruption in trends during the COVID-19 pandemic, we assigned a dummy variable in the model where a value of “1” was assigned to months in 2020 and “0” otherwise and tested for its statistical significance.

RESULTS

A total of 120,738 comments from 5827 users were collected from March 24, 2011, until August 31, 2022. We found 22 topics representing the cystic fibrosis community’s experiences and concerns. Our time series analysis showed that for 9 topics, the COVID-19 pandemic was a statistically significant event that disrupted the trends in user activity. Of the 9 topics, only 1 showed significantly increased activity during this period, while the other 8 showed decreased activity. This mixture of increased and decreased activity for these topics indicates a shift in attention or focus on discussion topics during this period.

CONCLUSIONS

There was a disruption in the experiences and concerns the cystic fibrosis community faced during the COVID-19 pandemic. By studying social media data, we were able to quickly and efficiently study the impact on the lived experiences and daily struggles of patients with cystic fibrosis. This study shows how social media data can be used as an alternative source of information to gain insight into the needs of patients with rare diseases and how external factors disrupt them.
Identification of potential key genes and their regulatory microRNAs and transcription factors in lymph node and skin metastasis in breast cancer using in silico analysis. (Preprint)
Authors: Gadwal Ashita , Purohit Purvi , Khokhar Manoj , Vishnoi Dr. Jeewan Ram , Pareek Dr. Puneet , Choudhary Dr. Ramkaran , Elhence Dr. Poonam , Mithu Banerjee1 Dr. Mithu , Sharma Dr. Praveen
Published: January 2023

BACKGROUND

Breast cancer with metastasis like lymph node and skin metastasis represents a significant cause of morbidity and mortality in patients. Patients with lymph node metastasis (LNM) has poor prognosis of the disease and likewise skin metastases may impair the quality of life due to physical appearance, odour, and bleeding. So therefore, it urgent to elucidate the key genes and pathways involved in lymph node and skin metastasis of breast cancer and its prognosis.

OBJECTIVE

In the present study, we aimed to identify the key genes that are involved in both lymph node and skin metastasis from the publicly available datasets and through survival analysis their role has been identified in breast cancer.

METHODS

GSE29431 and GSE53752 microarray gene expression datasets were obtained from the GEO (Gene Expression Omnibus) database, which included LNM and skin metastasis samples of breast cancer. Differentially expressed genes (DEGs) were obtained between lymph node and skin metastasis through GEO2R tool. Bioinformatical methods including Gene Ontology (GO) functional annotation analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis were performed using database for annotation, visualization and integrated discovery (DAVID) with the screened DEGs. The protein-protein interaction (PPI) network of the DEGs was constructed through Search Tool for the Retrieval of Interacting Genes database (STRING) website, and visualized through Cytoscape and HUB genes were identified Molecular Complex Detection (MCODE) and cytoHubba. Hub genes expression was analyzed for overall survival (OS) through Gene Expression Profiling Interactive Analysis (GEPIA). Their targeting miRNAs and transcription factors (TFs) were identified using mirNet.

RESULTS

In total 156 DEGs were identified. The GO function and KEGG pathway enrichment analyses indicated that these DEGs were enriched in extracellular matrix organisation, positive regulation of cell migration, immunoglobulin receptor binding, Wnt-protein binding. PPI was established with 142 nodes, 155 edges and through MCODE and Cytohubba thirteen Hub genes were identified. Four candidate genes MS4A1, CD79A, VPREB3, FCRLA had worse overall survival (OS) in breast cancer. A total 34miRNAs and 121TFs that target the candidate genes were identified through mirNet and hsa-mir-335-5p is found to be most significant miRNA that target three out of four hub genes.

CONCLUSIONS

Our study suggests that the four hub genes (MS4A1, CD79A, VPREB3, FCRLA) and 34 miRNAs are associated with lymph node and skin metastasis of breast cancer which can be a potential biomarker for therapeutics.
THE PHARMACOGNOSTIC STANDARDS, ANTIOXIDANT ACTIVITY, AND HEPATIC SAFETY PROFILE OF AN INDONESIAN ANTIDIABETIC POLYHERBAL FORMULATION
Authors: Hartanti Dwi
Published: January 2023

The excessive oxidative processes and the lack of cellular antioxidative mechanisms are significantly observed in diabetes. In addition, long-term medication required for the treatment might harm the hepatic tissues. This study evaluated the selected pharmacognostic characters, antioxidant activities, total phenolic content, and the hepatic safety of a polyherbal formulation containing seven plant constituents used by Klinik Wisata Kesehatan Jamu Kalibakung, Tegal, Indonesia, to treat diabetes patients. The pharmacognostic properties of the formulation were characterized according to the WHO quality control methods for herbal materials. The 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity (DPPH RSA), ferric reducing antioxidant power (FRAP), and total phenolic content (TPC) were evaluated as per the standard method. The effect of formulation on the hepatic HepG2 cells was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction assay. The pharmacognostic properties of the formulation specified as follow: foreign matters (1.32±0.05%), loss on drying (11.50±0.07%), total ash (5.68±0.07%), acid-insoluble ash (0.94±0.04%), water-soluble extractable (18.22±0.60%), and ethanol-soluble extractable (16.90±0.77%). The ethanol extract showed a superior DPPH RSA (960.70±2.58 mM Trolox equivalent (TE)/ g dry weight (DW)), FRAP (1112.69±8.39 mM TE/g DW), and TPC (1768.40±32.40 mg gallic acid equivalent (GAE)/g DW) over its water counterpart. However, the water extract was safer for HepG2 cells than the ethanol one, with the IC50 values of 218.25±14.03 and 40.24±3.53 µg/ml, respectively. This study set the pharmacognostic standards for an antidiabetic polyherbal formulation with excellent antioxidant activities, in which its traditional use as a decoction was safe for the hepatic cells.
The Effect of Xylocarpus granatum J. Koenig Seed Extract Cream on The Number of Fibroblast and Re-Epithelialization in IIA Degree Burn Wound Healing
Authors: Kusuma Wardani I Gusti Agung Ayu , Sanjaya Dwi Arymbhi , Suena Ni Made Dharma Shantini , Antari Ni Putu Udayana
Published: January 2023

Burns require a recovery process that multiplies complex biological mechanisms including anti-inflammatory, proliferation, and tissue remodeling. X.granatum J.Koenig (XG) is a popular plant that grows in the mangrove forest Bali. Traditionally, this plant is used mainly for antiinflammation, dysentery, fever, skin emolliating, wound treatment, and abdominal problems. Flavonoids, tannins and saponins are active metabolites of this plant, which has antiinflammation, antioxidant and wound healing effects. IL-1β, IL-6 and TNF-α are pro-inflammatory cytokines that are involved in the pathophysiology of wound. Fibroblasts support granulation tissue synthesis, ECM production, and collagen synthesis. Re-epithelialization plays a role in wound closure. The aim of present study to evaluate the effectiveness XG extract seeds cream on burn wound grade IIA in mice. XG extract was evaluated for antioxidant activity by DPPH method. The two cream formulations were measured for physical quality for organoleptic, homogeneity, and pH. The wound healing activity of XG creams were evaluated from wound contraction, histological analysis of fibroblast proliferation and re-epithelialization in mice. Statistical analyses were performed with post hoc Tukey using analysis of variance and were analyzed using Statistical Package for the Social Science (SPSS, Version 26). XG extract has a very strong antioxidant activity with an IC50 value of 7,939 ppm. The preparation of XG extract cream has homogeneous and pH of 6,5. The treatment with XGC 15% promote significant increases in wound contraction rate, proliferation of fibroblasts and re-epithelialization with SSD (p>0,05). These results suggest that XGC 15% is effective in wound healing in mice by increasing wound contraction rate, proliferation of fibroblasts and re-epithelialization.
Comparing Responses of Ursolic Acid in Murine Macrophages Infected with Mycobacterium smegmatis and Mycobacterium avium
Authors: Pitaloka Dian , Jihadah Muti’ah Nurul
Published: January 2023

Mycobacterium smegmatis and Mycobacterium avium offer an advantage in examining tuberculosis-like effects and host immune defense. Therefore, the study aims to examine the effect of ursolic acid (UA) on the host immune system by analyzing cytokines concentration, such as TNF-α, IL-6, IL-1β, and nitrite oxide produced by murine macrophages infected with Mycobacterium smegmatis and Mycobacterium avium. Femurs of female C57BL/6 mice aged 6–8 weeks were used to culture the Bone marrow-derived macrophages (BMDM). On day 10, BMDM was infected with Mycobacterium smegmatis and Mycobacterium avium using a multiplicity of infection (MOI) amounting to 8:1, then TNF-α, IL-6, and IL-1β were analyzed using ELISA and nitrite oxide with Griess reagent. The results showed that UA decreased the production of three respective pro-inflammatory cytokines used in the study, both in BMDM infected by Mycobacterium smegmatis and Mycobacterium avium. For TNF-α, the reduction was nearly 65%-90% compared to the control. The decrease in the production of IL-6 occurred from 2700 pg/ml to 750 pg/ml for BMDM infected with Mycobacterium smegmatis, while the reduction was more significant in those infected using Mycobacterium avium with approximately 150 pg/ml compared to the control. Moreover, UA reduced by over 90% of IL-1β and this result was in line with the reduction of nitrite.  UA decreases the production of pro-inflammatory cytokines such as TNF-α, IL-6, IL-1β, and nitrite. This result is preliminary but supports further study on the role of UA in immune defense from pathogenic and non-pathogenic mycobacterial infections.
Evaluation of Current Practice of Antibiotic Use and Clinical Outcomes of Community-Acquired Pneumonia Patients with Type 2 Diabetes Mellitus in Indonesia
Authors: Ariyanti Desi , Sauriasari Rani , Yunir Em
Published: January 2023

Impaired immunity in diabetes mellitus (DM) causes reduced lung function, which increases the incidence of various types of infections, including community-acquired pneumonia (CAP). The main principle of CAP therapy is the administration of antibiotics. This study aims to assess the appropriate use of antibiotics according to existing clinical practice guidelines using the Gyssen algorithm, and to determine its effect on clinical outcomest in hospitalized patients. It is an observational study with a retrospective cohort design and total sampling technique. The research was conducted at Dr. Cipto Mangunkusumo Hospital Jakarta, with the research subjects being 98 hospitalized CAP patients with type 2 DM (T2DM) over the period January 2018 - December 2019. The collection of data on patient characteristics and clinical outcomes was made through patient medical records. 28.6% of the subjects were in the severe category of CAP. The results of the evaluation using the Gyssen algorithm showed that 59.2% of the subjects receive the appropriate antibiotics, while 40.8%  receive non appropriate antibiotics. The clinical outcomes in the group receiving appropriate antibiotics (70.7%) was significantly higher than the non-appropriate group (42.5%). The results of the multivariate analysis show that the group receiving appropriate antibiotics experienced 2.7 times greater clinical outcomes (RR 2.683, 95% CI: 1.102-6.592), after controlling for the degree of CAP and the onset of antibiotic administration. The use of appropriate antibiotics in CAP patients with T2DM significantly enhanced clinical outcomes.
An Investigation on The Use of Colistin in Critically Ill Patients at A Teaching Hospital in Ho Chi Minh City
Authors: Vu Thi Lan Nhi , Dang Nguyen Doan Trang , Nguyen Tu Thien Tam
Published: January 2023

Colistin is reintroduced into therapeutic protocols as one of the last resort antibiotics against multidrug-resistant (MDR) pathogens. In February 2019, the International Consensus Guidelines (ICG 2019) on optimizing the administration of colistin has for the first time stipulated an official recommendation for higher colistin dosing regimen based on pharmacokinetic/pharmacodynamic (PK/PD) modeling data. This study aimed at assessing the current dosing of colistin at University Medical Center (UMC) of Ho Chi Minh City (HCMC) and to identify rates and risk factors of colistin-induced nephrotoxicity. A cross sectional study was conducted on patients admitted to Intensive Care Unit (ICU), being treated for severe infection with at least 5 days of colistin IV administration from April 2018 to April 2019. KDIGO criteria were used to evaluate nephrotoxicity during treatment. Colistin-resistance was detected in 4 cases during the study period. The majority (n=104, 87.4%) of patients was diagnosed with pneumonia due to mainly Acinetobacter baumannii. Rational dosing according to PK/PD approach, EMA, FDA was observed in 33 cases (27.8%), 39 cases (32,8%), and 44 cases (38,2%), respectively. 85 cases (71,4%) were evaluated as rational dosing in accordance with at least one of the three guidelines. Clinical efficacy was recorded in 59 infected patients (49.6%). Lower average maintenance dose was observed in patients with treatment failure (p = 0.002). KDIGO-defined acute kidney injury (AKI) developed in 70 patients (58,8%). Multivariate analysis showed that concomitant administration of vasopressors (OR = 16.52; 95% CI 5.3750.83; p = 0.001), furosemide (OR = 5.24; 95% CI 1.89–14.55; p = 0.001) and hypoalbuminemia (< 25 g/l) (OR = 6.24; 95% CI 2.17–17.93; p = 0.001) were significantly associated with nephrotoxicity. Consistent with previous studies, due to a very modest clinical cure rate with a high frequency of colistin-induced AKI and the development of colistin-resistant strains, alternative agents such as new beta-lactam/beta-lactamase inhibitor combinations with favourable safety data are strongly preferred for the treatment of carbapenem-resistant infection.
Synthesis and Antioxidant Actiivty of Some Dibenzylidene-Cyclohexanones
Authors: Ritmaleni Ritmaleni , Fatmayanti Risma Baiq , Ekananda Shinta Diva , Tranggono Bhagaskara Naufal , Arsani Nanda Kurnia , Rumiyati Rumiyati
Published: January 2023

Dibenzylidene-cyclohexanone is a curcumin analog. Analog of curcumins showed activity as antioxidant. This research is aimed to synthesize and study the antioxidant activity of dibenzylidene-cyclohexanone compounds. The synthesis was carried out according to carbonyl condensation reaction and its antioxidant activity was tested by using DPPH radical scavenging activity and FRAP assay. Compound entry 1 (2,6-bis-(3’-Bromo-4’-methoxybenzylidene)-cyclohexanone) and entry 3 (2,6-bis-(2’-chloro-6’-fluorobenzylidene)- cyclohexanone)  were obtained in 70 % yield. Compound entries 1 and 2 gave the same antioxidant activities according to the DPPH method and this result was not good enough compared to vitamin E. Compound entry 1 showed the best antioxidant activity among the series and also had a better antioxidant activity compared to vitamin E. From these results, it can be said that compound entry 1 was the best antioxidant agent in the series.
Peran Media Sosial dalam Membangun Kompetensi Literasi Sampah Generasi Muda di Kabupaten Sleman
Authors: Silaningrum Raras
Published: January 2023

Permasalahan sampah di Daerah Istimewa Yogyakarta (DIY) tak kunjung usai, salah satunya dikarenakan masyarakat tidak menyadari masalah yang timbul  dari sampah-sampah yang mereka hasilkan dalam jangka waktu panjang, terlihat dari ketidakpedulian mereka terhadap pengelolaan sampah masih tinggi. Media memegang peranan penting menginformasikan kepada publik mengenai hal tersebut, selain media, potensi lain pengentasan masalah sampah di DIY adalah pemuda sebagai aktor pembangunan. Kabupaten Sleman merupakan salah satu daerah yang menghasilkan sampah  terbesar di DIY, namun demikian mereka juga memiliki pemuda  dengan jumlah terbanyak yang dapat dilibatkan untuk mengentaskan masalah sampah. Fokus kajian ini yaitu menganalisis pengaruh peranan media sosial terhadap kompetensi literasi sampah generasi muda di Kabupaten Sleman. Jenis penelitian ini adalah deskriptif analisis dengan pendekatan kuantitatif survei. Lokasi penelitian berada di Kabupaten Sleman, populasinya adalah pemuda anggota karang taruna di Kabupaten Sleman. Hasil penelitian menunjukkan terdapat tiga jalur pengaruh positif peranan media sosial terhadap kompetensi literasi sampah yaitu jalur langsung tanpa perantara, jalur tidak langsung melalui sikap, dan jalur melalui pengetahuan dan sikap. Sementara itu, jalur tidak langsung melalui pengetahuan tidak terbukti signifikan. Hal ini dikarenakan pengetahuan yang dimiliki generasi muda tidak cukup komprehensif untuk merespons isu sampah. Berdasarkan tiga jalur pengaruh yang signifikan, jalur pengaruh langsung memiliki nilai korelasi yang paling kuat. Namun, peranan media sosial tersebut belum dirasakan secara optimal oleh generasi muda karena berada pada kategori kadang-kadang, sehingga pemanfaatannya masih perlu dioptimalkan.
Agronomic, economic and energy performance of cassava genotypes in the southwestern Amazon region
Authors: Passos Alexandre Martins Abdão dos , Quintino Simone Marçal , Costa Rogério Sebastião Correa da
Published: January 2023

Cassava is an important crop in many parts of the world. It is a staple food for millions of people, and it is also used in a variety of other applications. Cassava is a rich source of carbohydrates and can be used to produce fuel-grade ethanol. The purpose of this study was to evaluate the agronomic, economic, and energy performance of several cassava genotypes in the southwest region of the Brazilian Amazon. Root productivity, flour yield, dry mass, starch content, number of roots per plant, number of rotten roots per plant, plant and first branch height, gross and net costs and income, and the crop's energy balance were evaluated. The genotypes affected all evaluated characteristics. The BRS Kiriris genotype excelled in terms of flour yields, root productivity, and absence of rot disease. The highest net revenues were observed for the most productive genotypes. The research disclosed an average energy demand of 9.78 GJ ha-1. BRS Kiriris (252,7 GJ ha-1) produced the most favorable energy balance, with an energy efficiency of 26.9. The greatest demand for direct energy costs for root production was for nitrogen fertilization (37,9%), followed by the use of herbicides (27,9%), which have a high energy charge associated with their manufacture. Cassava is a valuable source of biofuel feed in the Brazilian Amazon's southwest region. The selection of the appropriate cassava genotype is crucial for achieving adequate levels of activity sustainability.
Determination of Age by Studying Radiological Fusion of Shoulder Joint in Relation to Birth Certificates in 16-20 Years Age Group
Authors: Srivastva Dr. Mahima , Ashwani Dr. , Mathur Dr. R. K.
Published: January 2023

Age estimation of an individual is of medicolegal importance. Age becomes an important criteria for voting, driving licence, marriage, in civil cases e.g. to get government jobs and pensions. In criminal cases, the judgment is based on the opinion about age given by the forensic experts. Ossification centres appear in a fairly definite sequence at a particular age group and fuses at a particular age group from which an age of the individual can be determined. Materials and methods: 60 subjects were randomly selected from various schools, from neighbourhood as well as the cases attending the OPD of General Medicine Department of Madras Medical College, Chennai – 3. Conclusion :None of the cases in the age group of 16 – 17 yrs among males showed fusion of the secondary ossification centre of the shoulder joint. 30.76% and 28.57% of the cases showed fusion of the secondary ossification centre of the shoulder joint among males in the age group of 17 – 18yrs and 18 – 19 yrs respectively. 30%, 40%, 50% of the cases showed fusion of the secondary ossification centre of the shoulder joint among females in the age group of 16 – 17 yrs, 17 – 18 yrs, 18 – 19 yrs respectively. In the present study, it is observed that the mean age at which the secondary ossification centre of the shoulder joint fuses is 18 – 19 yrs in both boys and girls. In the present study, the fusion of the secondary ossification centre of the shoulder joint occurred one year later among females in comparison to ages mentioned in various standard literatures.It is due to the racial, economic, hereditary and hormonal factors.
The Effect of Water-Saving Technologies on the Growth and Productivity of Rice in the Soil Climate of the Republic of Karakalpakstan
Authors: Erejepovich Kurbanbaev Sagit , Abbosovich Usmanov Shavkat , Bekmaxanovich Batyrbaev Erlan
Published: January 2023

Abstract: Based on the results of the research, the use of drip irrigation technologies in rice cultivation is based on the optimum irrigation regimes in accordance with the soil and climate conditions of the region, the mechanical composition of the soil, the level of salinity, the mineralization of the collector water, the effect on the growth and productivity of rice, the cultivation of rice using water-saving technologies in experimental options field and laboratory researches were carried out.
The Role of Marketing in Small Business and Entrepreneurial Activity
Authors: Boboniyazovich Axmedov Alim
Published: January 2023

Annotation: Marketing is the process of understanding consumer problems and regulating market activity and directing it to the goal. Small business enterprises with different forms of ownership should be closely connected with the marketing plan for production and sales activities. The marketing concept envisages making decisions in all spheres of activity of a small business enterprise in the market from the point of view of ensuring its overall success. This situation should be reflected in various types of organizational, management and sales work.