bluuebunny/crossref_metadata_2025 huggingface dataset:
Classification of post COVID-19 condition symptoms: a longitudinal study in the Belgian population
Authors: Nayani Sarah , Castanares-Zapatero Diego , De Pauw Robby , Van Cauteren Dieter , Demarest Stefaan , Drieskens Sabine , Cornelissen Laura , Devleesschauwer Brecht , De Ridder Karin , Charafeddine Rana , Smith Pierre
Published: October 2023

Objectives

Since the onset of the COVID-19 pandemic, most research has focused on its acute pathophysiology, yet some people tend to experience persisting symptoms beyond the acute phase of infection, referred to as post COVID-19 condition (PCC). However, evidence on PCC is still scarce. This study aimed to assess the distribution, classification of symptoms and associated factors of PCC in adults.

Design

Longitudinal online cohort study.

Setting

National study in Belgium.

Participants

Participants were Belgian adults with a recent SARS-CoV-2 infection and were recruited when called up for contact tracing. A total of 3039 participants were included and completed an online questionnaire at the time of their infection and again 3 months later.

Outcome measures

The baseline questionnaire assessed the initial health status of the participants and their status during the acute phase of the infection. The follow-up questionnaire assessed their PCC status 3 months after infection. A latent class analysis (LCA) was performed to assess whether there are different classes of individuals with a similar set of self-reported PCC symptoms.

Results

Half of the participants reported PCC 3 months after infection (47%). The most frequent symptoms were fatigue (21%), headache (11%) and memory problems (10%). The LCA highlighted three different classes of PCC symptoms with different risk factors: (1) a combination of loss of smell and taste, (2) a combination of neurological symptoms and (3) other heterogeneous symptoms.

Conclusions

With the increasing number of people who underwent COVID-19, PCC has become an important but complex public health problem due to the heterogeneity of its symptoms. The classification of symptoms performed in this study can help give insight into different aetiologies of PCC and better plan care according to the symptoms and needs of those affected.
Instruments for measuring patient health education competence among nursing personnel: protocol for a COSMIN-based systematic review
Authors: Wang Shuyi , Liu Ke , Shi Zeya , Chen Qirong , Tang Siyuan
Published: October 2023

Introduction

Health education, as a crucial strategic measure of disease prevention and control in the 21st century, has become an important part of healthcare. As the main deliverers of patient health education, nursing personnel’s patient health education competence (PHEC) has received much attention. Instruments for assessing the PHEC of nursing personnel have been developed internationally, but there is a lack of systematic reviews and evaluations of the psychometric properties of these instruments. To effectively select appropriate PHEC assessment instruments in specific contexts, a systematic and comprehensive review and evaluation of these measurement instruments are needed. The goal of this systematic review is to systematically evaluate the psychometric properties of existing PHEC instruments.

Methods and analysis

In this study, eight databases will be searched between 1 March 2023 and 31 2023 to retrieve studies that include instrument(s) measuring the PHEC of nursing personnel. Two researchers will independently perform literature screening, data extraction and literature evaluation. In case of disagreement, a third researcher will be involved in the resolution. The measurement properties of PHEC assessment instruments will be systematically reviewed based on the consensus-based standards for the selection of health measurement instruments (COMSIN) methodology and guideline.

Ethics and dissemination

Ethical approval is not applicable for this study. We will share the findings from the study at national and/or international conferences and in a peer-reviewed journal in the fields of health education and/or patient education.

PROSPERO registration number

CRD42023393293.
Acupuncture as a therapeutic intervention for post-COVID-19 vaccination urticaria: a systematic review and meta-analysis protocol
Authors: Wang Zixia , Xiao Yao , Wang Muyao , Chen Qiqi , Lin Yueqi , Fang Xinyue , Wang Wenhui
Published: October 2023

Introduction

The COVID-19 pandemic persisted for over 3 years since its onset in December 2019, posing an ongoing global threat to human health. In the absence of specific antiviral medications for COVID-19, vaccination has emerged as a popular preventive measure adopted by the general public. However, an undesirable consequence of COVID-19 vaccination has been the frequent incidence of urticaria, a type of adverse skin manifestations. Despite the prevalence of this issue, there is currently a lack of clinical evidence exploring the potential utility of acupuncture as a therapeutic approach to managing urticaria arising after COVID-19 vaccination. To address this knowledge gap, this study aims to comprehensively evaluate the effectiveness and safety of acupuncture as a therapeutic intervention for treating urticaria in the general population following COVID-19 vaccination.

Methods and analysis

The retrieval strategies employed in this study involve obtaining all relevant articles published from December 2019 to October 2023. These articles will be obtained from databases including PubMed, EMBASE, Cochrane Library, Web of Science, Chinese National Knowledge Infrastructure (CNKI), Chinese Biomedical Literature Database (SinoMed), VIP database and the WanFang database. Subsequently, the collected articles will undergo a thorough screening process based on predefined inclusion and exclusion criteria. Additionally, study quality will be evaluated using the Cochrane risk bias assessment tool. To conduct the meta-analysis, we will employ the Review Manager software (RevMan V.5.3). Finally, the study findings will be evaluated for their level of evidence.

Ethics and dissemination

As this is a secondary review of published clinical data, this study does not involve direct contact with human subjects, and therefore, ethical approval and consent are not required. The findings of the study will be disseminated through a peer-reviewed journal, ensuring that the results undergo rigorous evaluation by experts in the field.

PROSPERO registration number

CRD42022377343
Barriers to adherence to home-based pulmonary rehabilitation among patients with chronic obstructive pulmonary disease in Iran: a descriptive qualitative study
Authors: Sami Ramin , Salehi Kobra , Hashemi Marzieh , Haghighat Shila , Atashi Vajihe
Published: October 2023

Objectives

Chronic obstructive pulmonary disease is a chronic disease with numerous complications. Therefore, its effective management depends on home-based pulmonary rehabilitation. The effectiveness of home-based pulmonary rehabilitation programmes in turn depends on patient adherence to them. The aim of this study was to explore the barriers to adherence to home-based pulmonary rehabilitation among patients with chronic obstructive pulmonary disease.

Design

Qualitative descriptive design.

Setting

The lung care wards of Al-Zahra and Khorshid teaching hospitals and the comprehensive lung care clinic of Khorshid hospital, Isfahan, Iran.

Participants

Participants patients with chronic obstructive pulmonary disease (n=20), their family caregivers (n=15) and healthcare providers (n=14) recruited via purposive sampling.

Results

The five main categories of the barriers to adherence to home-based pulmonary rehabilitation among patients with chronic obstructive pulmonary disease were patient-related barriers, caregivers’ caregiving burden, limited support for patients, healthcare providers’ limited professional competence and ineffective home-based pulmonary rehabilitation planning.

Conclusion

Different and complex factors related to patients, families and healthcare providers can affect adherence to home-based pulmonary rehabilitation among patients with chronic obstructive pulmonary disease. Healthcare authorities can use the findings of this study to develop strategies to support patients with chronic obstructive pulmonary disease and their family caregivers and thereby reduce the physical and mental complications of Chronic obstructive pulmonary disease.
Cross-sectional study investigating the association between inflammatory biomarkers and neuropathy in adolescents with type 1 diabetes
Authors: Rasmussen Vinni Faber , Hirschberg Jensen Verena , Thrysøe Mathilde , Vestergaard Esben Thyssen , Størling Joachim , Kristensen Kurt
Published: October 2023

Objectives

The aims of this study were to investigate circulating levels of inflammatory markers in adolescents with type 1 diabetes with and without different types of neuropathies and evaluate the association between inflammatory biomarkers, nerve function and clinical parameters.

Design

Cross-sectional study.

Setting

Hospitals and Steno Diabetes Center in Denmark.

Participants

Adolescents with more than 5 years of diabetes duration were investigated for large fibre, small fibre and autonomic neuropathy as a part of the T1DANES study. Blood samples from the participants were analysed for inflammatory biomarkers by Meso Scale Discovery multiplexing technology.

Primary and secondary outcome measures

Inflammatory biomarkers and results of diagnostic nerve tests.

Results

Fifty-six adolescents with type 1 diabetes and 23 healthy controls were included. The adolescents with diabetes had significantly higher interferon-gamma, tumour necrosis factor-alpha (TNF-a), interleukin (IL)-10 and soluble urokinase plasminogen activator receptor (suPAR) compared with healthy controls (p values<0.05). TNF-a was higher in the adolescents with large fibre neuropathy (LFN) (p=0.03) compared with those without LFN in the group with diabetes. A negative correlation was seen between TNF-a and conduction velocity in nervus tibialis (p=0.04), and higher TNF-a and IL-6 were associated with higher gastric motility index (TNF-a, p value=0.03; IL-6, p value=0.02). There were no significant associations between inflammatory markers and expressed symptoms, haemoglobin A1c, diabetes duration or body mass index standard derivation score (p values>0.05). The receiver operating characteristic (ROC) curves for the inflammatory markers suggested them as poor screening methods for all types of neuropathies with an area under the curve between 0.47 and 0.67.

Conclusion

Our results confirm increased low-grade inflammation in adolescents with type 1 diabetes. TNF-a was higher in adolescents with LFN and correlated negatively with nervus tibialis conduction velocity. The other inflammatory biomarkers fail to support differences in those with and without different types of diabetic neuropathies. However, TNF-a and IL-6 were positively correlated to gastric motility index.
Left atrial appendage closure in very elderly patients in the French National Registry
Authors: Teiger Emmanuel , Eschalier Romain , Amabile Nicolas , Rioufol Gilles , Ducrocq Gregory , Garot Philippe , Lepillier Antoine , Bille Jacques , Elbaz Meyer , Defaye Pascal , Audureau Etienne , Le Corvoisier Philippe
Published: October 2023

Objective

Left atrial appendage closure (LAAC) is recommended to decrease the stroke risk in patients with atrial fibrillation and contraindications to anticoagulation. However, age-stratified data are scarce. The aim of this study was to provide information on the safety and efficacy of LAAC, with emphasis on the oldest patients.

Methods

A nationwide, prospective, multicentre, observational registry was established by 53 French cardiology centres in 2018–2021. The composite primary endpoint included ischaemic stroke, systemic embolism, and unexplained or cardiovascular death. Separate analyses were done in the groups <80 years and ≥80 years.

Results

Among the 1053 patients included, median age was 79.7 (73.6–84.3) years; 512 patients (48.6%) were aged ≥80 years. Procedure-related serious adverse events were non-significantly more common in octogenarians (7.0% vs 4.4% in patients aged <80 years, respectively; p=0.07). Despite a higher mean CHA2DS2-VASc score in octogenarians, the rate of thromboembolic events during the study was similar in both groups (3.0 vs 3.1/100 patient-years; p=0.85). By contrast, all-cause mortality was significantly higher in octogenarians (15.3 vs 10.1/100 patient-years, p<0.015), due to a higher rate of non-cardiovascular deaths (8.2 vs 4.9/100 patient-years, p=0.034). The rate of the primary endpoint was 8.1/100 patient-years overall with no statistically significant difference between age groups (9.4 and 7.0/100 patient-years; p=0.19).

Conclusion

Despite a higher mean CHA2DS2-VASc score in octogenarians, the rate of thromboembolic events after LAAC in this age group was similar to that in patients aged <80 years.

Trial registration number

ClinicalTrials.gov Registry (NCT03434015).
Gravitational lensing in a topologically charged Eddington-inspired Born–Infeld spacetime
Authors: Soares A. R. , Vitória R. L. L. , Pereira C. F. S.
Published: October 2023

In the present paper, we study several aspects of gravitational lensing caused by a topologically charged Monopole/Wormhole, both in the weak field limit and in the strong field limit. We calculate the light deflection and then use it to determine the observables, with which one can investigate the existence of these objects through observational tools. We emphasize that the presence of the topological charge produces changes in the observables in relation to the case of General Relativity Ellis–Bronnikov wormhole.
Magnetic shielding simulation for particle detection
Authors: Cabo Sara R. , Gómez Sergio Luis Suárez , Bonavera Laura , Sanchez Maria Luisa , Santos Jesús Daniel , de Cos Francisco Javier
Published: October 2023

Cherenkov-type particle detectors or scintillators use as a fundamental element photomultiplier tubes, whose efficiency decreases when subjected to the Earth’s magnetic field. This work develops a geomagnetic field compensation system based on coils for large scale cylindrical detectors. The effect of different parameters such as the size of the detector, the distance between coils or the magnetic field strength on the compensation using a basic coil system composed of circular and rectangular coils is studied. The addition of coils of very specific geometry and position to the basic configuration is proposed in order to address the compensation in the areas of the detector where it is more difficult to influence, in order to minimize the loss of efficiency. With such improvement, in the considered simulated system, more than 99.5% of the photomultiplier tubes in the detector experience an efficiency loss of less than 1% due to the effect of the magnetic fields.
Influence maximization based on double clusters multi-verse optimizer
Authors: Zhang Qiwen , Liu Yueyue , Ren Enliang
Published: October 2023

The influence maximization problem is one of the important research topics in the social network. We address the problems of meta-heuristic algorithms such as the high probability of entrapment in local optima, low accuracy of the solution, and decreasing diversity in the late iteration. The Double Clusters Multi-Verse Optimizer (DCMVO) algorithm is proposed as a solution to the problem of influence maximization. In DCMVO, based on the fact that nodes in the social network are susceptible to the influence of neighboring nodes, individuals in the globular cluster are updated using neighboring nodes with high similarity, which enhances local exploitation and improves the accuracy of the solution. To improve the global exploration, using a comprehensive learning strategy in the open cluster enables individuals to learn from surrounding individuals by dimensions, thereby expanding the search space. The wormhole mechanism is used to enhance the information interaction between double clusters during the iterative process, which serves to balance local exploitation and global exploration. Under the independent cascade (IC) model, extensive experiments conducted on seven actual social networks demonstrate the effectiveness of DCMVO.
Vacuum stability conditions of the general two-Higgs-doublet potential
Authors: Song Yisheng
Published: October 2023

In this paper, we present the novel analytical expressions for the bounded-from-below or the vacuum stability conditions of scalar potential for a general two-Higgs-doublet model by using the concepts of co-positivity and the gauge orbit spaces. More precisely, several sufficient conditions and necessary conditions are established for the vacuum stability of the general 2HDM potential, respectively. We also give an equivalent condition of the vacuum stability of the general 2HDM potential in theory, and then, apply it to derive the analytical necessary conditions of the general 2HDM potential. Meanwhile, the semi-positive definiteness is proved for a class of 4th-order two-dimensional complex tensor.
Multi-Scale Feature Refined Network for Human Pose Estimation
Authors: Yang Qiaoning , Ji Xiaodong , Yang Xiuhui
Published: October 2023

Occlusive keypoints has been a challenge for human pose estimation, especially the mutual occlusion of human bodies. One possible solution to this problem is to utilize multi-scale features, where small scale features are capable of identifying keypoints, while large-scale features can capture the relationship between keypoints. Feature fusion among multi-scale features allows for the exchange of information between keypoints, facilitating the inference of occluded keypoints based on the identified keypoints. However, it’s found that there are invalid features in feature fusion which will interfere valid feature. In this paper, we propose multi-scale feature refined network (MSFRNet) based on HRNet and a new attention module namely multi-resolution attention module (MRAM). The proposed MRAM is designed to strengthen the effective information while suppressing redundant information. It has multiple inputs and outputs and can learn the relationships between keypoints while retaining detailed information. The proposed MSFRNet outperforms HRNet, achieving a 1.4[Formula: see text]AP improvement on the COCO dataset with only a marginal computational increase of 0.35 GFLOPs. Additionally, it demonstrates superior performance with a 0.9[Formula: see text]AP, 0.7[Formula: see text]AP, and 1.8[Formula: see text]AP improvement on the MPII, CrowdPose and OCHuman datasets, respectively. Furthermore, compared with the latest attention mechanism PSA, the MSFRNet exhibits lower computational cost while maintaining the same pose-estimation accuracy.
A Robust Kernel Least Mean Square Algorithm and its Quantization
Authors: Huo Yuan-Lian , Liu Jie , Qi Yong-Feng , Hu Zhi-Ling , Yang Kuo-Jian
Published: October 2023

To further improve the performance of the kernel adaptive filtering algorithm in a non-Gaussian environment, a robust kernel least mean square algorithm is proposed, and the effectiveness of the root cost function and the convergence of the algorithm is theoretically analyzed. An improved online vector quantization criterion is then applied to the proposed algorithm to suppress the linearly growing network size. Finally, the different performances of the algorithm of this paper and other kernel adaptive filtering algorithms as well as this paper’s algorithm before and after quantization are compared in Mackey Glass chaotic time series as well as in system identification, confirming the superiority of the algorithm of this paper and the improved online vector quantization criterion.
Multisensor Fusion SLAM Research Based on Improved RBPF‐SLAM Algorithm
Authors: Li Nan , Zhou Feng , Yao Kaiwen , Hu Xinli , Wang Rugang
Published: October 2023

Simultaneous localization and map construction (SLAM) technology provides the foundation for indoor robots to realize autonomous path planning. The Rao‐Blackwellized particle filtering (RBPF) algorithm is widely used to obtain information and perform map construction in unknown environments. This paper proposes a multisensor fusion algorithm that improves the RBPF‐SLAM algorithm by addressing the issues of particle distribution errors and degradation. To achieve this, the extended Kalman filter (EKF) is first adopted to effectively fuse odometry and inertial navigation (inertial measurement unit (IMU)) data as the initial positional information. Then, a high‐precision light detection and ranging (LIDAR) observation model is integrated to calculate the proposed distribution, and a threshold is introduced to determine the number of valid particles to simplify the resampling step. Finally, raster map construction experiments were carried out on both the Gazebo simulation environment and Robot Operating System (ROS)‐based mobile robot Keepbot platform in different scenarios. The experimental results demonstrate that the optimized RBPF‐SLAM algorithm generates a clearer and more complete map outline, which can effectively improve the accuracy of robot pose estimation, acquire a reliable 2D raster map with a reduced number of particles, and greatly reduces the computational effort.
Femoral Neck Fracture in a Pediatric Patient with Primary Hyperparathyroidism
Authors: Schmitt Mark W. , Modrak Maxwell J. , Bouza Soumar J. , Smith Brian G. , Adrados Murillo A.
Published: October 2023

Case. A previously healthy 11-year-old girl underwent expedited surgical fixation of a femoral neck fracture sustained while jump-roping. After further work up, she was diagnosed with primary hyperparathyroidism. Parathyroidectomy of a hypertrophic adenoma proved curative. Now, five months post left hip surgery, the patient is pain-free and walks without a limp. Conclusion. We describe the first published case of primary hyperparathyroidism presenting as a pathologic hip fracture in a child. Although presentation with a fracture is exceedingly rare, bone pain is a frequent complaint of pediatric hyperparathyroidism. Orthopedic surgeons may find themselves the front-line caregivers for the condition.
Direct and Binder-Free MXene-Assisted Cobalt Manganese Phosphate Electrode Fabrication on Carbon Cloth by Electrosynthesis for Efficient Supercapacitors
Authors: Hussain Sajjad , Katkar Pranav K. , Vikraman Dhanasekaran , Sheikh Zulfqar Ali , Nazir Ghazanfar , Batoo Khalid Mujasam , Kim Deok-Kee , Kim Hyun-Seok , Jung Jongwan
Published: October 2023

In this work, MXene-assisted cobalt manganese phosphate (MXene-CMP) thin films with various Co/Mn compositions were prepared on a carbon cloth (CC) substrate via an easy and efficient low-cost electrodeposition route. MXene-CMP thin films were further used as polymer-free active materials for hybrid supercapacitors. The structural, compositional, and surface properties of the prepared MXene-CMP hybrid thin films were characterized using the latest analytical methods. The H-3 electrode exhibited enhanced specific capacitance (840 F·g-1 at 2 A·g-1) with exceptional robust performance. Additionally, a symmetric H-3 MXene-CMP supercapacitor device also demonstrated a high energy density of 48 Wh·kg-1 at 1.5 kW·kg-1 power density and holds better cyclability (93% of its initial value after 5000 cycles). Finally, this work explored the strong potential of MXene-attached bimetallic phosphates for solving the electrochemical shortcomings of energy storage devices.
Eco-Speed Harmonization with Partially Connected and Automated Traffic at an Isolated Intersection
Authors: Kang Zhen , An Lianhua , Lai Jintao , Yang Xiaoguang , Sun Wensheng
Published: October 2023

This paper proposed an eco-speed harmonization method at intersections. It is able to reduce carbon emissions by controlling partially connected and automated traffic and signal timing. It has the following features: (i) traffic emission reduction enhancement at various demand levels; (ii) traffic emission achievement while improving the mobility of entire traffic at intersections; (iii) enhanced traffic emission reduction with the help of a small portion of connected and automated vehicles; and (iv) potential implementations in the near feature. To validate the effectiveness, the proposed method is evaluated against a state-of-the-art strategy. Sensitivity analysis is conducted under various demand levels and market penetration rates (MPRs) of connected and automated vehicles (CAVs). The result shows that the proposed method outperforms and has the benefits of traffic emission reduction, throughput improvement, and stop frequency reduction. The proposed method demonstrates consistent performance across all demand levels and CAVs’ MPR. The proposed approach can achieve a reduction in emissions ranging from 4% to 61%, an average increase in throughput of around 14.91%, and a decrease in the stop frequency of at least 26%. This provides the foundation for future CAVs-based eco-approaching strategies.
Supplementary Table S4 from Targeting MALAT1 Augments Sensitivity to PARP Inhibition by Impairing Homologous Recombination in Prostate Cancer
Authors: Yadav Anjali , Biswas Tanay , Praveen Ayush , Ganguly Promit , Bhattacharyya Ankita , Verma Ayushi , Datta Dipak , Ateeq Bushra
Published: October 2023

Supplementary Table S4: Computational prediction of microRNAs with putative binding sites on the MALAT1transcript.
Supplementary Table S2 from Targeting MALAT1 Augments Sensitivity to PARP Inhibition by Impairing Homologous Recombination in Prostate Cancer
Authors: Yadav Anjali , Biswas Tanay , Praveen Ayush , Ganguly Promit , Bhattacharyya Ankita , Verma Ayushi , Datta Dipak , Ateeq Bushra
Published: October 2023

Supplementary Table S2: Genes upregulated in metastatic prostate cancer patients in comparison to localized cases.
Supplementary Methods from Targeting MALAT1 Augments Sensitivity to PARP Inhibition by Impairing Homologous Recombination in Prostate Cancer
Authors: Yadav Anjali , Biswas Tanay , Praveen Ayush , Ganguly Promit , Bhattacharyya Ankita , Verma Ayushi , Datta Dipak , Ateeq Bushra
Published: October 2023

Supplementary Methods
Supplementary Figure S6 from Targeting MALAT1 Augments Sensitivity to PARP Inhibition by Impairing Homologous Recombination in Prostate Cancer
Authors: Yadav Anjali , Biswas Tanay , Praveen Ayush , Ganguly Promit , Bhattacharyya Ankita , Verma Ayushi , Datta Dipak , Ateeq Bushra
Published: October 2023

MALAT1 depletion enhances sensitivity to PARP inhibitors in HR proficient PCa cells.
Supplementary Figure S5 from Targeting MALAT1 Augments Sensitivity to PARP Inhibition by Impairing Homologous Recombination in Prostate Cancer
Authors: Yadav Anjali , Biswas Tanay , Praveen Ayush , Ganguly Promit , Bhattacharyya Ankita , Verma Ayushi , Datta Dipak , Ateeq Bushra
Published: October 2023

MALAT1 does not interact with HR proteins.
Supplementary Figure S3 from Targeting MALAT1 Augments Sensitivity to PARP Inhibition by Impairing Homologous Recombination in Prostate Cancer
Authors: Yadav Anjali , Biswas Tanay , Praveen Ayush , Ganguly Promit , Bhattacharyya Ankita , Verma Ayushi , Datta Dipak , Ateeq Bushra
Published: October 2023

MALAT1 regulates the DNA repair pathway in prostate cancer.
FIGURE 5 from Targeting MALAT1 Augments Sensitivity to PARP Inhibition by Impairing Homologous Recombination in Prostate Cancer
Authors: Yadav Anjali , Biswas Tanay , Praveen Ayush , Ganguly Promit , Bhattacharyya Ankita , Verma Ayushi , Datta Dipak , Ateeq Bushra
Published: October 2023

MALAT1 modulates the expression of HR genes by sponging tumor-suppressive miR-421. A, Venn diagram displaying the miRNAs predicted to bind to MALAT1 transcript using four miRNA prediction tools binding computational tools, namely LncBaseV.2, miRanda, NPInter v4.0, and miRTar. B, Mature miR-421 sequence and its seed sites within 3′UTR of BRCA1, ATM and RAD51. The seed sequence of miR-421 is shown in red while the target sequence is depicted in blue. C, Bar plot depicting the relative expression of miR-421 and MALAT1 in 22RV1-miR-421 cells and control cells. D, qPCR depicting relative expression of HR genes in the same cells as in C. E, Quantitative PCR depicting relative expression of HR genes in 22RV1-shSCRM and -shMALAT1 cells transfected with nontargeting antagomiR or antagomiR-421. F, Schematic illustrating the predicted miR-421–binding sites near the 3′ end of MALAT1. G, Illustration of luciferase reporter construct with the wild-type or mutated (transformed residues in red) miR-421–binding sites on MALAT1 3′ end downstream of the firefly luciferase reporter gene. H, Bar plots depicting the luciferase reporter activity in HEK293T cells cotransfected with MALAT1-WT or MALAT1-mut construct with nontargeting mimics or miR-421 mimic. I, RIP followed by real-time qPCR analyses demonstrating enrichment of MALAT1 and miR-421 with AGO2 antibody–bound beads in comparison to IgG (control antibody) in 22RV1 cells. The experiments were performed in triplicate with biologically independent samples (n = 3); the data represent mean ± SEM. The statistical analysis of differences was calculated using one-way ANOVA with Dunnett multiple-comparisons posthoc test for panels C–E and H–I.
FIGURE 3 from Targeting MALAT1 Augments Sensitivity to PARP Inhibition by Impairing Homologous Recombination in Prostate Cancer
Authors: Yadav Anjali , Biswas Tanay , Praveen Ayush , Ganguly Promit , Bhattacharyya Ankita , Verma Ayushi , Datta Dipak , Ateeq Bushra
Published: October 2023

MALAT1 depletion impairs HR-mediated DSB repair in prostate cancer cells. A, DAVID analysis depicting biological pathways downregulated in LNCaP-abl-siMALAT1 cells relative to LNCaP-abl–siCTL. Bars represent -log10 (P) and the frequency polygon (line in orange) represents the number of genes. B, Correlogram depicting Pearson correlation coefficient (ρ) between DNA repair–associated genes and MALAT1 in prostate cancer patient samples from GSE35988 and GSE3325 datasets (FDR adjusted, P < 0.05). Correlation coefficients are expressed by the color from red to blue and the dot size is proportional to the strength of the correlation. Representative genes are marked on the sides of the correlogram. C, Representative confocal images for γH2AX foci (green) in control and MALAT1-ablated 22RV1 and LNCaP cells. The nucleus was visualized by DAPI (blue). Scale bar, 10 µm. D, Quantification of the number of γH2AX-positive foci in the indicated cells. Bar plot showing the percentage of cells with the indicated number of foci/nuclei in the same cells. The P value for the χ2 test is indicated. E, Immunoblots showing the expression of HR markers in MALAT1-silenced and shSCRM prostate cancer cells. β-Actin was used as a loading control. F, Same as C, except immunostaining for BRCA1. G, Same as C, except immuno-staining for RAD51. H, Schematic of the pDR-GFP reporter used to monitor HR activity in 22RV1 MALAT1-KO and shRAD51 cells. Bar plot exhibiting the percentage of GFP+ cells in 22RV1-MALAT1-KO and shRAD51 cells transfected with pDR-GFP reporter construct. Experiments were performed with n = 3 biologically independent samples; the data represent mean ± SEM. For D and F–H, one-way ANOVA with Dunnett multiple comparisons posthoc test was applied while the χ2 test was used for D.
FIGURE 2 from Targeting MALAT1 Augments Sensitivity to PARP Inhibition by Impairing Homologous Recombination in Prostate Cancer
Authors: Yadav Anjali , Biswas Tanay , Praveen Ayush , Ganguly Promit , Bhattacharyya Ankita , Verma Ayushi , Datta Dipak , Ateeq Bushra
Published: October 2023

MALAT1 promotes EMT, stemness, and chemoresistance in prostate cancer. A, Immunoblots showing the expression of EMT markers in shMALAT1 and shSCRM prostate cancer cells. β-Actin was used as an internal control. B, Boyden chamber Matrigel migration assay using the same cells as in A. Representative fields with the migrated cells are shown in the inset. The bar plot depicts the alteration in migratory potential of the prostate cancer cells upon MALAT1 knockdown. C, Flow cytometry analysis showing expression of CD117 (c-KIT) and CD133 in 22RV1-shMALAT1 and shSCRM control cells. D, Immunofluorescence images displaying the expression of CD117 and CD44 and in the same cells as in C. Scale bar, 20 µm. E, Dot plot represents quantification of CD117 and CD44 mean fluorescence intensity (MFI) per unit area shown as arbitrary units (AU). F, Representative phase-contrast images for the prostatospheres formed using 22RV1-shMALAT1 and shSCRM control cells on the indicated days. Scale bar, 100 µm. G, Bar plot superimposed with dots represents the mean area of the prostatospheres and percentage sphere formation efficiency. H, qPCR depicting the expression of stem cell markers in the prostatospheres derived from the same cells as in E. Expression level for each gene was normalized to GAPDH. I, Flow cytometry analysis showing expression of ATP-binding cassette superfamily G member 2 (CD338/ABCG2) using the same cells as in C. J, Cell cytotoxicity assay using chemotherapeutic drugs namely, doxorubicin and 5-fluorouracil using the same cells as in C. IC50 values were calculated by generating a dose–response curve using GraphPad Prism software. Experiments were performed with n = 3 biologically independent samples; the data represents mean ± SEM. The statistical difference among the groups was computed using one-way ANOVA with Dunnett multiple-comparison test for B, E, G, and H.
Vincristine-Induced Seizure from Drug Interactions: A Case Report and Review of Literature
Authors: Heidari Shima , Panahishokouh Mahsa , Babakhani Davoud , Aghabeigi Sohrab , Vaezi Mohammad , Amini Shahideh , Hadjibabaie Molouk , Shahrami Bita
Published: October 2023

Vincristine (VCR) as a key drug for the treatment of acute lymphoblastic leukemia (ALL) is associated with neurotoxicity. We present a young man with a history of controlled childhood seizures who was diagnosed with pre-B-cell ALL and developed generalized tonic-clonic seizures following the Cancer and Leukemia Group B (CALGB) 8811 regimen. The patient also received oral itraconazole to prevent fungal infection initiated by chemotherapy. Possible causes of seizure, including electrolyte abnormalities, hypoglycemia, central nervous system infection or inflammation, were ruled out. According to the Naranjo Adverse Drug Reaction Scale, the patient’s seizure had been attributed to VCR, possibly secondary to concomitant use of itraconazole and doxorubicin. The patient successfully recovered after discontinuation of VCR and supportive care. Clinicians should be aware of the possibility of vincristine-induced seizure in adult patients, especially with the concomitant use of drugs known to have potential drug-drug interactions.
The Effect of Gender on Identification and Interpretation of Non-Suicidal Self-Injury
Authors: Curtis Cate , Terry Michaela
Published: October 2023

Introduction: Reported rates of non-suicidal self-injury (NSSI) differ by gender but may be under-reported and under-recognised in men. People engaging in NSSI rarely seek professional help without encouragement, so others play a key role in its identification and potential intervention. The current research investigated others’ interpretations of NSSI, examining whether gender affects the likelihood of NSSI identification and views of how common and acceptable NSSI is. Method: Participants (N = 429; 74.1% female, 23.3% male; please see below for further demographic information) responded to two vignettes describing a person self-injuring by punching a wall or by cutting themselves. The person’s gender in each vignette was manipulated. Following each vignette, the participants rated the level to which they agreed the behaviour was common for the gender of the person described, as well as the level to which they agreed the behaviour was acceptable for the gender of the person described, on a 5-point Likert scale. Following both vignettes, participants were presented with a definition of NSSI and rated the level to which they agreed cutting and wall-punching were forms of NSSI on 5-point Likert scales. Independent-samples t tests and goodness of fit χ2 tests were conducted as appropriate. Results: Participants were more likely to identify wall-punching as common for men and cutting as common for women. However, there was no significant difference in whether wall-punching was identified as NSSI or considered to be an acceptable behaviour, regardless of the gender of the person engaging in it. That is, although research suggests that men are far more likely to engage in wall-punching as a form of NSSI than women, participants did not recognise this. Overall, the results indicated a gender-dependent difference in how acceptable and common NSSI is thought to be, but no noticeable difference in identification of a behaviour as NSSI. Wall-punching, typically a form of NSSI engaged in by males, tended not to be identified as such. Conclusion: There is an effect of gender on how NSSI is interpreted, and it seems that men’s NSSI is, and will continue to be, under-recognised. This has important implications for the treatment of men’s NSSI, which is more likely to be seen as aggression and therefore deserving of punishment than an attempt at emotion regulation.
Proceedings of the 2022 UAB CRRT Academy: Non-Invasive Hemodynamic Monitoring to Guide Fluid Removal with CRRT and Proliferation of Extracorporeal Blood Purification Devices
Authors: Teixeira J. Pedro , Zeidman Amanda , Beaubien-Souligny William , Cerdá Jorge , Connor Jr. Michael J. , Eggleston Katrina , Juncos Luis A. , da Silva J. Ricardo , Wells Catherine , Yessayan Lenar , Barker Andrew B. , McConville Wendy , Speer Rajesh , Wille Keith M. , Neyra Javier A. , Tolwani Ashita
Published: October 2023

In 2022, we celebrated the 15th anniversary of the University of Alabama at Birmingham (UAB) Continuous Renal Replacement Therapy (CRRT) Academy, a 2-day conference attended yearly by an international audience of over 100 nephrology, critical care, and multidisciplinary trainees and practitioners. This year, we introduce the proceedings of the UAB CRRT Academy, a yearly review of select emerging topics in the field of critical care nephrology that feature prominently in the conference. First, we review the rapidly evolving field of non-invasive hemodynamic monitoring and its potential to guide fluid removal by renal replacement therapy (RRT). We begin by summarizing the accumulating data associating fluid overload with harm in critical illness and the potential for harm from end-organ hypoperfusion caused by excessive fluid removal with RRT, underscoring the importance of accurate, dynamic assessment of volume status. We describe four applications of point-of-care ultrasound used to identify patients in need of urgent fluid removal or likely to tolerate fluid removal: lung ultrasound, inferior vena cava ultrasound, venous excess ultrasonography, and Doppler of the left ventricular outflow track to estimate stroke volume. We briefly introduce other minimally invasive hemodynamic monitoring technologies before concluding that additional prospective data are urgently needed to adapt these technologies to the specific task of fluid removal by RRT and to learn how best to integrate them into practical fluid-management strategies. Second, we focus on the growth of novel extracorporeal blood purification devices, starting with brief reviews of the inflammatory underpinnings of multiorgan dysfunction and the specific applications of pathogen, endotoxin, and/or cytokine removal and immunomodulation. Finally, we review a series of specific adsorptive technologies, several of which have seen substantial clinical use during the COVID-19 pandemic, describing their mechanisms of target removal, the limited existing data supporting their efficacy, ongoing and future studies, and the need for additional prospective trials.
Trends in Accuracy and Appropriateness of Alopecia Areata Information Obtained from a Popular Online Large Language Model, ChatGPT
Authors: O'Hagan Ross , Kim Randie H. , Abittan Brian J. , Caldas Stella , Ungar Jonathan , Ungar Benjamin
Published: October 2023

Background: Patients with alopecia areata (AA) may access a wide range of sources for information about AA, including the recently developed ChatGPT. Assessing the quality of health information provided by these sources is crucial, as patients are utilizing them in increasing numbers. Objectives: The aim of the study was to evaluate appropriateness and accuracy of responses to common patient questions about AA generated by ChatGPT. Methods: Responses generated by ChatGPT 3.5 and ChatGPT 4.0 to 25 questions addressing common patient concerns were assessed by multiple attending dermatologists in an academic center for appropriateness and accuracy. Appropriateness of responses by both models for use in two hypothetical contexts as follows: (1) for patient-facing general information websites, and (2) for electronic health record (EHR) message drafts. Results: The accuracy across all responses was 4.41 out of 5. Accuracy scores of responses ChatGPT 3.5 responses had a mean of 4.29, whereas those generated by ChatGPT 4.0 had mean accuracy score of 4.53. Assessments ranged from 100% of responses rated as appropriate for the general question category to 79% questions about management for an EHR message draft. Raters largely preferred responses generated by ChatGPT 4.0 versus ChatGPT 3.5. Reviewer agreement was found to be moderate across all questions, with a 53.7% agreement and Fleiss’ κ co-efficient of 0.522 (p value <0.001). Conclusions: The large language model ChatGPT outputted mostly appropriate information for common patient concerns. While not all responses were accurate, the trend toward improvement with newer iterations suggests potential future utility for patients and dermatologists.
KEANDALAN VALIDITY VERIFICATION SYSTEM BUKTI TRANSAKSI:MAMPUKAH MENCEGAH FRAUD ACCOUNTING?
Authors: Ginting Rafles , Aswat Ibnu , Malini Helma
Published: October 2023

Fraud is defined as an intentional act of deception done by one or more people. This fraud can occur in management, either directly or through a third party. Many things can be done by the company in the deployment of accounting fraud prevention. One of them is confirming the validity of transaction evidence, which is critical since transaction evidence is basic transaction papers, both self-made and from third parties, that are used as sources for a company's recording or preparation of financial statements. Proof of transactions is critical in averting the creation of future financial issues. Fraud detection uses machine learning, statistical analysis, and behavioral tracking to uncover the patterns and strategies criminals use to commit fraud. When fraud is detected, the system can prevent fraud before it causes damage to your business or organization. It can be used to prove that an entity or organization has a good anti-fraud system in place.
IMPLEMENTASI SISTEM PENGENDALIAN MUTU PADA KANTOR AKUNTAN PUBLIK ABC DENGAN BASIS VALUE
Authors: Rahmawati Rika Yulia , Hastuti Theresia Dwi
Published: October 2023

The purpose of this research is to finding out how Sistem Pengendalian Mutu (SPM) is applied to a Kantor Akuntan Publik (KAP), how KAP redesigns SPM implementation based on standards and values and aims to see how the application of SPM affects audit quality. This study uses all individuals in the KAP ABC as informants, including one Partner, one Supervisor and six Auditor Staff. Research data was collected by interviewing all informants. Research data was analyzed manually and supported by Nvivo software tools to improve the quality of analysis. The results of this study indicate that in KAP ABC there are values (independent value, confidentiality value, family value and cohesive value) that are well internalized, but the implementation of SPM in KAP ABC has not been carried out optimally resulting in less than optimal audit quality.
PERBANDINGAN PERLAKUAN PAJAK DAN ZAKAT PADA PRODUK KEUANGAN ISLAMI MUDHARABAH DAN MURABAHAH
Authors: Rismawati Rismawati , Supriadi Supriadi
Published: October 2023

This paper aims to describe the past and present tax and zakat obligations imposed on each Muslim as two different obligations through a literature review. Taxes are levied by the government on every citizen including Muslims with the aim of assisting the government in financing the state. While zakat is paid by Muslims based on religious obligations aimed at increasing the devotion of the Ummah, reducing poverty and increasing solidarity and building economic sustainability. Using a descriptive method, this study found that in contemporary Islamic economics, zakat is used as a tool to improve the economy with an interest-free loan system (qardul hasan). Mudharabah and Murabahah are both Islamic financial products designed by considering aspects of sharia compliance, including the fulfillment of zakat obligations. The basic principle of zakat in Islam is the obligation to give a portion of wealth to those who deserve it. Meanwhile, the imposition of taxes on Islamic financial products has the same purpose as the imposition of taxes on conventional financial products, namely to finance government spending in providing public services to the public. The payment of zakat through official institutions can be submitted as a tax deduction, so that the tax functions as the main source of state revenue and is used entirely for the benefit of the people in line with the purpose of zakat payment in Islam.
ANALISIS EFISIENSI BIAYA PRODUKSI MIE PADA UD MIE UJANG KABUPATEN JEMBER
Authors: Isnaini Nadia , Kantun Sri , Herlindawati Dwi
Published: October 2023

This study aims to measure the level of efficiency of raw noodle production costs at UD Mie Ujang, Jember Regency with standard costs as a reference in the use of production costs. This research belongs to the type of quantitative descriptive research. The type of data used is the main data in the form of production cost report documents of UD Mie Ujang, Jember Regency for the first quarter of 2022 and supporting data in the form of interview results related to the condition of production cost reports. The research informants are the owners and employees of UD Mie Ujang, Jember Regency. The results showed that the use of raw noodle production costs at UD Mie Ujang, Jember Regency in the first quarter of 2022 showed efficient results. The use of production costs is more efficient in producing super raw noodles compared to ordinary raw noodles. The results of cost efficiency can be used as a consideration and decision in determining the selling price of the next product.
A survey of Sumatran Ctenidae (Araneae). 2. Three new species of Bowie Jäger, 2022
Authors: OMELKO MIKHAIL M. , FOMICHEV ALEXANDER A.
Published: October 2023

Three new species, Bowie beruang sp. nov., B. binturong sp. nov., and B. dhole sp. nov., are described from Sumatra (Indonesia). Each species description is based on both sexes. Detailed descriptions, digital photographs and a distributional map are provided.
Taxonomic notes on the genus Charterginus Fox, 1898 (Vespidae: Polistinae: Epiponini) with images of the type specimens and new occurrence records
Authors: SOMAVILLA ALEXANDRE , ARAGÃO MARCOS , BARROSO PAULO CÉZAR SALGADO , MENEZES RODOLPHO SANTOS TELLES , CARPENTER JAMES M.
Published: October 2023

Charterginus Fox, 1898 is a neotropical genus of swarm-founding social wasps consisting of six species. Despite its ecological significance, there are limited studies on various aspects of these wasps. To address this, this study aims to provide a comprehensive comparative diagnosis for all Charterginus species, accompanied by high-quality images of the type material. Moreover, a distribution map incorporating both previous and newly recorded occurrences is presented, shedding light on the geographical range of these species. Through these efforts, we aim to enhance the knowledge and facilitate future research on Charterginus, ultimately contributing to a better understanding of swarm-founding social wasps.