bluuebunny/crossref_metadata_2025 huggingface dataset:
Whole-transcriptome analysis of serum neuron-derived exosomes reveals specific signature for autism spectrum disorder in Chinese children
Authors: Qin Yannan , Cao Li , Zhang Haiqing , Cai Shuang , Zhang Jinyuan , Guo Bo , Wu Fei , Zhao Lingyu , Li Wen , Ni Lei , Liu Liying , Wang Xiaofei , Chen Yanni , HUANG CHEN
Published: July 2021

BackgroundAutism spectrum disorder (ASD) is a prevalent developmental disorder that appears in early childhood and manifests repetitive behavioral and social deficits. Reported biomarkers for ASD cannot directly and specifically reflect the abnormality of brain neurons which are preferentially affected and correlated with clinical severity of the disease. Neuron-derived exosomes (NDEs), which carry proteins, lipids and nucleic acids can reflect a variety of brain diseases. However, little research has been reported about NDEs in children with ASD. MethodsSerum samples were collected from 100 ASD children and 60 age-matched typically developed (TD) children, and were pooled into 5 ASD groups and 3 TD groups (n=20). NDEs from the pooled sera in each group were isolated using L1CAM antibody mediated immunosorbent assay and characterized by nanoparticle tracking analysis, transmission electron microscopy and western blot. Whole-transcriptome of NDEs was detected by lncRNA microarray and RNA-Sequencing. RNAs expressed differently in NDEs from ASD sera as versus those from TD sera were screened, analyzed, and validated. ResultsSerum NDEs were isolated successfully. A total of 1418 mRNAs, 1745 lncRNAs and 11 miRNAs were identified to be differentially expressed, and most of them were down-regulated in ASD. The differentially expressed RNAs were prone to be distributed on chromosome 17. Target gene prediction and bioinformatic analysis revealed that a large portion of RNAs were involved in neuron- and glycan-related networks implicated in ASD. qRT-PCR identified that the levels of EDNRA, SLC17A6, HTR3A, OSTC, TMEM165, PC-5p-139289_26, and hsa-miR-193a-5p changed significantly in ASD, indicating them as potential biomarkers for the disease. LimitationsThe correlation between the expression of candidate biomarkers and disease severity were not examined. Some of our participants were not medication-free. Samples were mainly from a single hospital. ConclusionsWhole-transcriptome analysis of serum NDEs reveals specific signature for ASD in Chinese children, which may help detect predictive biomarkers and molecular mechanisms of ASD, and provide reference for diagnoses and therapeutic management of the disease.
Corn Starch Reactive Blending with Latex from Natural Rubber in Presence of Na+ Ions of Carboxymethyl Cellulose as a Catalyst
Authors: Leksawasdi Noppol , Chaiyaso Thanongsak , Rachtanapun Pornchai , Jantrawut Pensak , Ruksiriwanich Warintorn , Seesuriyachan Phisit , Phimolsiripol Yuthana , Techapun Charin , Sommano Sarana Rose , Ougizawa Toshiaki , Jantanasakulwong Kittisak
Published: July 2021

Corn starch (CS) was blended with glycerol, latex natural rubber (LNR), and carboxymethyl cellulose (CMC). The addition of 10 phr of CMC improved the Young’s modulus (6.7 MPa), tensile strength (8 MPa), and elongation at break (80%) of the CSG/LNR blend. The morphology of the CSG/LNR/CMC blends showed a uniform distribution of LNR particles (1–3 µm) in the CSG matrix. The addition of CMC enhanced the swelling ability and water droplet contact angle of the blends owing to the swelling properties, interfacial crosslinking, and amphiphilic structure of CMC. Fourier transform infrared spectroscopy confirmed the reaction between the C=C bond of LNR and the carboxyl groups (–COO-) of CMC, in which Na+ ions in CMC acted as a catalyst. Notably, the mechanical properties of the CSG/LNR/CMC blend were improved owing to the miscibility of CSG/CMC and the CMC/LNR interfacial reaction.
The essential role of nuclear translocation of β-catenin in the osteoblastic differentiation of giant cell tumor of bone: Prediction of tumor ossification after denosumab treatment
Authors: Kimura Atsushi , Toda Yu , Matsumoto Yoshihiro , Yamamoto Hidetaka , Yahiro Kenichiro , Shimada Eijiro , Kanahori Masaya , Fukushima Suguru , Nakagawa Makoto , Setsu Nokitaka , Endo Makoto , Fujiwara Toshifumi , Matsunobu Tomoya , Oda Yoshinao , Nakashima Yasuharu
Published: July 2021

BackgroundDenosumab is a game-changing drug for giant cell tumor of bone (GCTB); however, its clinical biomarker in terms of post-administration ossification of the tumors has not been elucidated. The aims of this study were to investigate the relationship between Wnt/β-catenin signaling and the ossification of GCTB and to evaluate whether endogenous nuclear β-catenin expression predicted denosumab-induced bone formation in GCTB. MethodsWe established cultures of patient-derived primary GCTB stromal cells (pGCTB-SCs) and assessed the osteoblastic characteristics of pGCTB-SCs using cytochemical staining, optical density method (405 nm), and immunocytochemistry. Several mRNA expressions of osteoblastic markers (ALP, collagen type Ⅰ alpha 1 (COL1A1), integrin-binding sialoprotein (IBSP), runt-related transcription factor2 (RUNX2), and bone gamma-carboxyglutamate protein (BGLAP) was also investigated using quantitative real-time PCR (qRT-PCR) analysis. The impact on nuclear β-catenin translocation (NBT) by differentiation-induction was evaluated using Western blotting and immunocytochemistry. Wnt signaling inhibitor, GGTI-286, and selective Rac1 inhibitor, NSC23766, were added with OGM to evaluate the essential role of NBT. Furthermore, the distribution of endogenous NBT and the relationship with the tumoral ossification were assessed. Its predictability for the ossification in GCTB was also evaluated using a nuclear β-catenin labeling index (NBLI) of clinical samples (n = 86) and quantitative analyses of CT images.ResultspGCTB-SCs significantly expressed the osteoblastic markers with osteogenic medium (OGM) in a time-dependent manner (P < 0.0001, vs. vehicle). NBT was upregulated within 12 hours after OGM treatment. In addition, decreasing NBT by treatment with 40μM GGTI-286 significantly abolished the osteoblastic differentiation of pGCTB-SCs (P < 0.0001, vs. OGM). The selective Rac1 inhibitor, NSC23766, also suppressed the OGM-induced osteoblastic differentiation and NBT in pGCTB-SCs. Furthermore, in GCTB clinical samples, the NBTs were significantly associated with the tumor ossification following denosumab treatment (P = 0.003 for entire and peripheral tumor, and P = 0.02 for intra-tumor of GCTB) as well as endogenous intra-tumoral ossification. ConclusionsIn summary, our findings suggest a close relationship between the NBT and the osteoblastic differentiation of GCTB. Investigations of NBTs in naïve GCTB samples will provide a promising biomarker for predicting the ossification of GCTB following denosumab treatment.
Design and Analysis of a Novel Schönflies Motion Parallel Robot with Full Cycle Rotation
Authors: Li Luquan , Fang Yuefa , Wang Lin , Yao Jiaqiang
Published: July 2021

Due to the complex structures of multi-limbed parallel robots, conventional parallel robots generally have limited workspace, complex kinematics, and complex dynamics, which increases the application difficulty of parallel robot in industrial engineering. To solve the above problems, this paper proposes a single-loop Schönflies motion parallel robot with full cycle rotation, the robot can generate Schönflies motion by the most simplified structure. The novel Schönflies motion parallel robot is a two-limb parallel mechanism with least links and joints, and each limb is driven by a 2-degree of freedom (DOF) cylindrical driver (C-driver). The full cycle rotation of the output link is achieved by “…R-H…” structure, where the revolute (R) and helical (H) joints are coaxial. Mobility, kinematics, workspace and singularity analysis of novel Schönflies motion parallel robot are analyzed. Then, dynamic model is formulated based on the principle of virtual work. Moreover, a pick-and-place task is implemented by proposed Schönflies motion parallel robot and a serial SCARA robot, respectively. The simulation results verify the correctness of the theoretical model. Furthermore, dynamics performances of Schönflies motion parallel robot and serial SCARA robot are compared, which reveal the performance merits of proposed Schönflies motion parallel robot.
Liver Transplantation in Children and Impact of Intra-operative Goal Directed Therapies on Postoperative Outcome
Authors: Kumba Claudine
Published: July 2021

Background: Liver transplantation is a hemorrhagic surgery with high postoperative complication rates in terms of organ dysfunction and infections. We conducted a retrospective observational monocentric study that had the objectives of determining predictors of postoperative outcome in children. In the initial cohort, 19 children underwent liver transplantation. A description of the postoperative outcomes of these patients was undertaken.Objective: To describe intraoperative and postoperative outcomes in children undergoing liver transplantation and to propose intraoperative management implementation measures for postoperative outcome improvement.Methods: Secondary analysis of liver transplanted patients included in the initial study.Results: There were 19 patients who underwent liver transplantation with a median age of 31[2-154] months. Five patients had re-operations (26.3%). Fourteen (73.7%) had intraoperative and/or postoperative complications. Five (26.3%) had intraoperative hemorrhagic shock. One (5.3%) had postoperative respiratory failure, one (5.3%) had postoperative multiple organ failure, two (10.5%) had postoperative neurologic failure and five (26.3%) had cardio-circulatory failure. One (5.3%) had postoperative pulmonary sepsis, five (26.3%) had postoperative abdominal sepsis, and six (31.6%) had postoperative septicemia. Intra-operative transfusion rate was 100%. In-hospital mortality rate was 10.5% (two patients).Conclusion:There were multiple postoperative adverse outcome predictors. Optimal intraoperative patient management with transfusion guided by point-of-care viscoelastic devices and goal-directed fluid and hemodynamic therapy with validated parameters and tools in children is a possible pathway to upgrade postoperative evolution in pediatric liver transplantation.
Differences in affect integration in children with and without internalizing difficulties
Authors: Fiskum Charlotte , Andersen Tonje Grønning , Johns Unni Tanum , Jacobsen Karl
Published: July 2021

Background:

Affect represents an important source of information about our internal state and the external world that can motivate and vitalize us. When affect is poorly integrated, this can lead to problems with self-regulation and psychopathology. Few studies have investigated affect integration in children.

Objective:

This study investigates differences in affect integration in children with and without internalizing difficulties.

Method:

Thirty-three Norwegian children (aged 9–13) with and 24 children without internalizing difficulties were interviewed with the Affect Consciousness Interview (ACI), a measure of affect integration. Data from the ACI was analyzed across nine affective categories (Interest/Excitement, Enjoyment/Joy, Fear/Panic, Anger/Rage, Shame/Humiliation, Sadness/Despair, Envy/Jealousy, Guilt/Remorse, and Tenderness/Care), and four dimensions (Awareness, Tolerance, Emotional, and Conceptual expressivity).

Results:

The children differed significantly in affect integration across all dimensions and all assessed affects, both positive and negative. Emotional Expressivity, Anger/Rage, and Sadness/Despair were particularly less integrated in the children with internalizing problems.

Conclusions:

Assessment of affect integration can provide useful information on possible underlying factors in internalizing problems in children and may help guide and personalize therapeutic interventions. Based on knowledge from empirical infant psychology interventions mimicking rich, early intersubjective experiences are recommended to increase affect integration.
DEVELOPMENT AND APPLICATION OF A TEST MIXTURE FOR UNTARGETED LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY ANALYSIS OF URINE SAMPLES
Authors: Torres Clarisse , Sardela Vinicius , Scalco Fernanda , Aquino Francisco , Garrett Rafael
Published: July 2021

Metabolic profiling of complex biological matrices based on liquid chromatography-mass spectrometry (LC-MS) allows detecting a wide range of metabolites with distinct concentrations and physicochemical properties. Given the complexity of samples and the necessity of a comprehensive approach in untargeted metabolomics, quality control strategies are mandatory to obtain high-quality data. The LC-MS performance must be monitored and evaluated to guarantee data reliability. In this study, a test mixture (TM) was developed, systematically evaluated, and applied to untargeted metabolomics of urine samples from individuals suspected of inborn errors of metabolism. The TM was composed of fifteen analytes that eluted across the entire gradient in reversed-phase columns and ionized in positive/negative electrospray modes. It helped set the LC-MS conditions for urine analysis, from sample reconstitution solvent to selecting the MS ion source parameters. The TM quickly indicated column stationary phase degradation during the batch analysis when employed to monitor and evaluate the LC-MS system in an untargeted metabolomic analysis. Thus, in addition to pooled QC samples, a TM can be employed in untargeted metabolomics to rapidly assess the system performance avoiding unnecessary efforts for further data treatment and multivariate analysis of poor-quality data
Advanced hybrid electro-oxidation & O3 technology for water reuse in the fruit and vegetable process industry
Authors: Sanchis Sonia , Gali David , Sala Vicent , Gomez Pedro , Pinedo Javier , Donato Javier , Berlanga Jose Guillermo , Garcia-Montaño Julia
Published: July 2021

Alternative sanitising agents are required in the fruit and vegetable processing industry, capable of effectively disinfecting both the product and the washing water to increase its reuse, while not producing harmful by-products like chlorine-based agents. In the present work, electro-oxidation (EO) and ozone are proposed for vegetable process water reuse, being optimised for their individual and combined application. The application of hybrid electro-oxidation and ozone achieved 7 log reduction after 15 min of treatment in batch using boron-doped diamond (BDD) as anode material, showing important synergistic effects when compared with the individual treatments. When the process was applied in continuous mode, up to 6 log reduction of total bacteria was achieved using EO alone with Ru as the anode and stainless steel as the cathode, under 11 mA/cm2 of current density, a cell retention time of 12 s and no addition of chemical reagents (electrolyte). Under these conditions, the absence of microorganisms in water was maintained for more than 24 h, no harmful by-products (chlorate, bromate) were detected and no damage to lettuce seeds were observed when evaluating water reuse potential.
Using Postmarket Surveillance to Assess Safety-Related Events in a Digital Rehabilitation App (Kaia App): Observational Study
Authors: Jain Deeptee , Norman Kevin , Werner Zachary , Makovoz Bar , Baker Turner , Huber Stephan
Published: July 2021

Background

Low back pain (LBP) affects nearly 4 out of 5 individuals during their lifetime and is the leading cause of disability globally. Digital therapeutics are emerging as effective treatment options for individuals experiencing LBP. Despite the growth of evidence demonstrating the benefits of these therapeutics in reducing LBP and improving functional outcomes, little data has been systematically collected on their safety profiles.

Objective

This study aims to evaluate the safety profile of a multidisciplinary digital therapeutic for LBP, the Kaia App, by performing a comprehensive assessment of reported adverse events (AEs) by users as captured by a standardized process for postmarket surveillance.

Methods

All users of a multidisciplinary digital app that includes physiotherapy, mindfulness techniques, and education for LBP (Kaia App) from 2018 to 2019 were included. Relevant messages sent by users via the app were collected according to a standard operating procedure regulating postmarket surveillance of the device. These messages were then analyzed to determine if they described an adverse event (AE). Messages describing an AE were then categorized based on the type of AE, its seriousness, and its relatedness to the app, and they were described by numerical counts. User demographics, including age and gender, and data on app use were collected and evaluated to determine if they were risk factors for increased AE reporting.

Results

Of the 138,337 active users of the Kaia App, 125 (0.09%) reported at least one AE. Users reported 0.00014 AEs per active day on the app. The most common nonserious AE reported was increased pain. Other nonserious AEs reported included muscle issues, unpleasant sensations, headache, dizziness, and sleep disturbances. One serious AE, a surgery, was reported. Details of the event and its connection to the intervention were not obtainable, as the user did not provide more information when asked to do so; therefore, it was considered to be possibly related to the intervention. There was no relationship between gender and AE reporting (P>.99). Users aged 25 to 34 years had reduced odds (odds ratio [OR] 0.31, 95% CI 0.08-0.95; P=.03) of reporting AEs, while users aged 55 to 65 years (OR 2.53, 95% CI 1.36-4.84, P=.002) and ≥75 years (OR 4.36, 95% CI 1.07-13.26; P=.02) had increased odds. AEs were most frequently reported by users who had 0 to 99 active days on the app, and less frequently reported by users with more active days on the app.

Conclusions

This study on the Kaia App provides the first comprehensive assessment of reported AEs associated with real-world use of digital therapeutics for lower back pain. The overall rate of reported AEs was very low, but significant reporting bias is likely to be present. The AEs reported were generally consistent with those described for in-person therapies for LBP.
Web-Based Structured Education for Type 2 Diabetes: Interdisciplinary User-Centered Design Approach (Preprint)
Authors: Poduval Shoba , Ross Jamie , Pal Kingshuk , Newhouse Nikki , Hamilton Fiona , Murray Elizabeth
Published: July 2021

BACKGROUND

Digital health research encompasses methods from human-computer interaction and health research.

OBJECTIVE

This paper aims to describe how these methods were combined to develop HeLP-Diabetes: Starting Out, a web-based structured education program for people newly diagnosed with type 2 diabetes.

METHODS

The development process consisted of three phases: initial design for effectiveness, optimization for usability, and in the wild testing in the National Health Service with people newly diagnosed with type 2 diabetes, and further revisions. We adopted an iterative user-centered approach and followed steps from the human-computer interaction design life cycle and the Medical Research Council guidelines on developing and evaluating complex interventions.

RESULTS

The initial design process resulted in an 8-session program containing information and behavior change techniques targeting weight loss, being more active, and taking medication. The usability testing was highlighted at an early stage, where changes needed to be made to the language and layout of the program. The in the wild testing provided data on uptake of and barriers to use. The study suggested low uptake and completion of the program, but those who used it seemed to benefit from it. The qualitative findings suggested that barriers to use included an expectation that the program would take too long. This informed refinements to the program.

CONCLUSIONS

The use of interdisciplinary methods resulted in an iterative development process and refinements to the program that were based on user needs and data on uptake. The final intervention was more suitable for a definitive evaluation than the initial version. The description of our approach informs other digital health researchers on how to make interventions more sensitive to user needs.
Utility and acceptability of text messages for PMTCT: a qualitative assessment of user experiences (Preprint)
Authors: Brown Melinda , Maloba May , Wexler Catherine , Mabachi Natabhona , Koech Sharon , Lagat Silas , Kamau Yvonne , Goggin Kathy , Gautney Brad , Finocchario-Kessler Sarah
Published: July 2021

BACKGROUND

Evidence that text messages can support patient adherence to HIV care is growing. We conducted a pilot study of the HIV Infant Tracking System (HITSystem 2.0), an intervention that includes SMS to patients to support maternal medication adherence, appointment attendance, and hospital-based deliveries during the antenatal period.

OBJECTIVE

The objective of this study was to qualitatively assess women’s experiences receiving SMS to understand message utility and acceptability with the goal of refining and strengthening the intervention.

METHODS

As part of an intervention development pilot study, we conducted semi-structured interviews with 33 women living with HIV who received SMS through the HITSystem 2.0 throughout their pregnancy and postpartum period. Interview questions assessed women’s feedback of the SMS, the impact of the SMS on their PMTCT care, and suggestions for improvement.

RESULTS

Participants described how SMS reinforced existing strategies for medication adherence and served as cues to action for antenatal appointments and hospital-based deliveries. SMS also provided encouragement and enhanced their sense of engagement with their clinical care team. Given the neutral content of the messages, most women reported no concerns about message confidentiality. Recommendations to enhance utility included greater customizability (timing and frequency of messages) and a few women suggested more comprehensive counseling regarding the SMS content to anticipate at the time of enrollment.

CONCLUSIONS

SMS content was deemed useful and acceptable by pregnant and postpartum women living with HIV and engaged in PMTCT services. Participant recommendations to increase message customization options have been adopted to enhance the intervention.

CLINICALTRIAL

Trial registration: clinicaltrials.gov, NCT02726607. Registered 01 April 2016. Retrospectively registered. https://clinicaltrials.gov/ct2/show/NCT02726607
Mapping Maternal Health in the New Media Environment: A scientometric Analysis in CiteSpace (Preprint)
Authors: Xie Yinghua , Long Chengxu , Lang Dong , Tang Shangfeng
Published: July 2021

BACKGROUND

The new media provides a convenient digital platform to access, use and exchange health information. As a special group of health care, maternal is still of international concern due to their high mortality rate. Improving maternal health as a Millennium Development Goal of the United Nations is an important quest for the health care system. Scientific research provides advice on how to improve maternal health through stringent reasoning and accurate data. However, the dramatic increase of publications, the diversity of themes, and the dispersion of researchers may reduce efficiency.

OBJECTIVE

This study aims to analyze the research progress on maternal health under the global new media environment, exploring the current research hotspots and research frontiers.

METHODS

A scientometric analysis was carried out by CiteSpace5.7.R1, searching in the core database of Web of Science for articles published in English from 1998 to 2021, and combined topic words such as new media, maternal, and health. In total, 3312 articles have been retrieved, of which 2270 studies have been included for further analysis. Top countries and institutions, potentially high-impact literature, research frontiers, and hotspots were analyzed in this study.

RESULTS

The number of publications grew rapidly after 2008, from 29 publications sharply increasing to 472 publications by 2020. Research centers concentrated in Latin America, such as the University of Toronto, the University of California. The work of Larsson M, Lagan BM, Tiedje L, and Helle C had a high potential impact. Most of the research subjects were maternal and newborn babies, and the research frontiers focused on health education and maternal psychological problems. Maternal mental health, maternal and infant nutrition, weight, production technology, and equipment were hotspots.

CONCLUSIONS

The development of new media has brought a new era for maternal health, characterized by psychological qualities, healthy and reasonable physical conditions, and advanced technology.
Trust Based Approach to Counter Attacks on WSN
Authors: Garg Palak
Published: July 2021

Wireless sensor networks (WSN) have a developing future due to their minimal expense, power-efficiency, and simple to-carry out attributes. Be that as it may, its security issues have become an interesting issue of exploration these days. This article inspects the headway of system of countering assaults where the responsibility, leftover energy of hub and examination of effective and fruitless occasions are consider while registering the trust level. Unlike existing work, this paper talk about the technique in which the trust esteem per layer of a sensor hub is assessed by the deviations of key boundaries at every convention layer considering the attacks. The execution of the proposed counter measure is then investigated utilizing the t-conveyance. Reproduction of WSN has been done to approve the outcomes. With the assistance of reenactment results, it has been seen that the proposed trust-based methodology for interruption location beats existing plans to the extent of revealing safety breaks in WSN.
Internet Of Things (IOT) Based Smart Health Monitoring System
Authors: Karmakar Sougata
Published: July 2021

IOT is one of the flourishing fields in coming years and it has a vital role in the health care sector. IOT helps us to connect with people by collecting major parameters of the patients directly through some wearable devices transmitted to smartphones and laptops of the authorized person using the cloud server. We are using devices which gives flexible operations to both for the patients and also for healthcare professionals. IOT is slowly becoming a trend in recent times by improvement in the wireless sensor networks. We are fetching such parameters like body temperature, oxygen saturation percentage, heart rate by using NodeMCU WIFI module and cloud computing. Patients with serious health issues can be quickly identified and can be provide a rapid solution by this health monitoring system. And by using BLYNK mobile application we can have those measurements of the parameters from anywhere in the world.
Random Mobility based Network to Handle Attacks in Vehicular System
Authors: . Pooja
Published: July 2021

This paper focuses on improved mobility based routing to handle attacks in VANET. It provides study of reconfiguration concept in vehicular system. All vehicles are moving with random speed and controlled by neuro fuzzy system. It provides improvement in mobility based routing to improve performance in VANET. Due to this, it focuses on improved mobility based routing that helps to prevent attacks in system. The proposed system is compared with actual system in terms of performance parameters. All simulations are be presented in MATLAB tool.
French Translations of the Qur’an Between Aesthetics and Scholarly Accuracy
Authors: Mukhetdinov D. V.
Published: July 2021

This article examines the history of the tradition of translation of the Qur’an into French. This tradition is one of the oldest and most inﬂuential, as it has signiﬁcantly supported the development of many other traditions of translations of the Qur’an, primarily, but by no means only, into many European languages (including Russian). In this paper we have attempted to examine the history of the tradition of translating the Qur’an into French in the context of the searching for a balance between the aesthetic characteristics of the interpreted text and its scholarly adequacy. Particular attention has also been paid to the translations of the Qur’an into French made by Francophone Muslims themselves: both in France and abroad.
Kitab Patanjali (Introdiction)
Authors: Rayhan al-Biruni Abu
Published: July 2021

Transl.: Ruzana V. Pskhu, Valentina N. Putyagina.
Combined Mutations of DMD and CFTR Genes in an Azerbaijani Family
Authors: Saltanat Aghayeva  , Lala Huseynova  , Hagverdiyeva  Raya
Published: July 2021

A  9  year  old  boy  with  the  obvious  traits  of  Duchene  muscular  dystrophy  disease  is  a  resident  of  Masalli  region  of Azerbaijan,  located  in the  south-east  foothill  of Talysh  Mountains.  Family  members  such  as mother,  father  and sister  of this  index patient  were examined. To diagnose all members of the family, biochemical analysis was conducted for quantitative analysis of creatine phosphokinase  in blood serum and genealogical  survey identified  inherited  disease  cystic fibrosis  in first cousin of this index patient  . Quantitative  evaluation  of creatine  phosphokinase  in blood serum was performed  for all family members  of the index patient   ., and identified high values: in index patient (2298 U/L, norm - 38-137 U/L), in his mother (879 U/L, norm - 26-140 U/L) and his sister (852U/L, norm - 26-140 U/L), whereas his father had normal range values of creatine phosphokinase  in blood (53,1 U/L, where norm - 38-137  U/L).  Diagnosis  of Duchenne  muscular  dystrophy  disease  was  confirmed  in index  patient  in hemizygous  state.  His mother  and sister were found as heterozygous  carriers of the (Duchenne  muscular dystrophy)  DMD gene. Molecular  genetic analysis of the DMD gene (MLPA) identified mutation in the mother and sister of the index patient. Mutation type was nonsense, and classified as pathogenic class. Molecular genetic analysis of the DMD gene showed a gain of mutations, consisting of two copies encompassing  exon 03 to 09 in the index patient, mother and his sister. The identified two different mutations of DMD gene in Azerbaijani family: fragment deletion of exon 45 in three sibs from Astara region of Azerbaijan, located in the south-east of the country, and deletion encompassing  exons from 8 to 20 in 10-year-old  boy in Balakan  region,  located  in the north-west  of the Republic  Mutation  type is a nonsense  mutation  and classified as pathogenic of class 1. Inherited cystic fibrosis in heterozygous  state was additionally identified in the index patient, in father and sister. The screening  of identified  mutations  may serve as a prenatal  diagnostic  tool to carefully  plan the prophylaxis  in patients with   cystic fibrosis. Furthermore,  our studies may serve as a basis for the future investigation  of many aberrant molecular mechanisms and regulatory  pathways.  The study of Duchenne  and Becker  muscular  dystrophy  resulted  in one of the first successful  attempts  at reverse genetics, better described as positional cloning, in humans. Discovery and subsequent analysis of the gene mutation that results in the clinical disorder led to the discovery of the encoded protein, dystrophin. This coinage set a precedent for the naming of proteins discovered by positional cloning of human disease genes: for example, huntingtin, emerin, and ataxin
Tools for Developing a Regional Education Development Strategy
Authors: Lapygin Yu. N. , Boltunov S. A.
Published: July 2021

The framework structure of the federal law on strategic planning leaves a wide field of activity for developers to use various tools in the process of building a development strategy for both the region and its sub-systems, which include the education system of the region. The methodological recommendations of the Ministry of Economic Development of the Russian Federation regarding the development of a strategy specify only the content of the main directions of the strategy, but allow the use of various methods, models and mechanisms for developing the strategy itself. Therefore, the study of the issues of instrumental support of procedures for developing strategies for regions and its subsystems is of interest from a theoretical and practical point of view.

In the study of theoretical and applied problems of instrumental support of the process of developing a strategy, algorithms are built in the form of a general scheme for developing a strategy and performing a comprehensive analysis prior to development. Algorithms for developing a strategy at the initial stage were formed based on the results of a comprehensive analysis, at the stage focused on the main goal (Mission), as well as at the final stage, the goals of which are focused on the ideal state in the future — Vision. Besides. An algorithm for determining the expected results has been built. on the basis of which a scenario for the development of regional subsystems is built.

The scientific novelty of the developed procedures lies in the fact that a set of tools for determining strategically significant projects and programs of the final and intermediate stages of strategy implementation is proposed; tools for agreeing strategically significant decisions were formed throughout the cycle of strategic development of the region’s education system; shows the procedure for constructing a tree of goals for the intermediate stage of the strategy implementation; a procedure for the formation of a development strategy at the intermediate stage of strategy implementation has been developed. The proposed approach to building a strategy for the development of socio-economic systems in the region is implemented in the process of adjusting the development strategy of the Vladimir region in terms of vocational education in the region.
The Need for Regional Labor Markets for Mid-Level Specialists and the Priorities of Graduates of Secondary Vocational Education in Employment Issues
Authors: Lomteva E. V. , Bedareva L. Yu. , Polushkina A. O.
Published: July 2021

The article considers the problems of imbalance of supply and demand in regional labor markets, as well as the role of career guidance in choosing a profession. The article discloses the main management decisions for regulating reception targets, taking into account labor market requirements. The authors give examples of calculating the imbalance of supply and demand in personnel in regional labor markets using the example of the region’s need for mid-level medical workers. The article considers the aspects of employment of graduates of professional educational organizations, including labor migration and international experience. As a result of the study, the authors identified and formulated the general laws of labor migration of graduates of professional educational organizations and solutions to reduce the outflow of labor resources from the region. Separately, the authors examined the employment of graduates of the SVE (secondary vocational education) system in Moscow and St. Petersburg. The article was completed within the framework of the topic of the State Task of the RANEPA N 11.5 “Study of models for the transformation of regional systems of secondary vocational education and their need for resource support” for 2021.
Theory and Practice of Primary Adaptation as a Factor in Retaining Young Specialists at the Enterprises of the Mining Industry in Russia
Authors: Kovalchuk D. M. , Smagin M. A.
Published: July 2021

The article is devoted to the urgent problem of retaining young specialists at the enterprises of the Russian mining industry. It defines the importance of such an important area of work of personnel management services, as primary adaptation, for the successful solution of this problem. The complex content of adaptation programs for young specialists is revealed and the main strategies for their retention in modern conditions are outlined. At the theoretical level of consideration of the problem, general scientific research methods were used, as well as specific sociological analysis and content analysis of the works of leading Russian scientists in the field of personnel management. The applied significance of the article is to describe the practical work of the personnel management services of the Joint Stock Company ALROSA to retain young specialists at the mining industry enterprises.
Gestão do SUS na perspectiva da literatura: fragilidades, potencialidades e propostas
Authors: Iglesias Alexandra , Andreatta Paloma Pereira , Drumond Nadilu Cardoso , Garcia Daniella Caldas , Ribeiro Neto Pedro Machado
Published: July 2021

The consolidation of Unified Health System (“SUS”) demands the democratization of relationships; however, in its 30 years, the separation between management and health care has been remarkable. Thus, the objective was to know how scientific articles address health management in SUS. An integrative literature review was carried out in the Scielo and PePSIC databases with the descriptors: “health management”; “co-management”; and “participatory management”, with 16 articles remaining. Thematic content analysis followed, resulting in the categories: “conceptions about health management in SUS”; “difficulties for health management”; “potentialities of health management”; and “proposals for health management”. We concluded that is necessary to bring social actors closer together and consider aspects such as affectivity and subjectivity in the management process, allowing workers, managers and users - all SUS operators - to recognize themselves in health production.
Penambahan Tepung Kedelai Pada Roti Tawar Tepung Sorgum dan Pati Garut Bebas Gluten dengan Zat Besi dan Serat Pangan
Authors: Khoirunnisa Wilda , Fauziyah A’immatul , Nasrullah Nanang
Published: July 2021

Kedelai merupakan biji-bijian dengan zat besi dan serat pangan sehingga dapat menaikkan kadar zat  besi dan serat pangan pada produk bebas gluten untuk aman dikonsumsi oleh penderita penyakit celiac wanita dewasa. Penelitian ini bertujuan untuk menganalisis pengaruh penambahan tepung kedelai terhadap kadar zat besi dan serat pangan, mengetahui sifat organoleptik, memperoleh formula terpilih, dan mengetahui kandungan zat gizi per takaran saji formula terpilih. Penelitian ini menggunakan desain Rancangan Acak Lengkap (RAL). Analisis data kadar zat besi dan serat pangan menggunakan uji One Way ANOVA (analisis ragam) dan uji lanjut Duncan Multiple Range Test (DMRT). Analisis uji organoleptik diolah secara deskriptif. Pemilihan formula terpilih menggunakan Metode Perbandingan Eksponensial (MPE). Hasil uji anova menunjukkan bahwa penambahan tepung kedelai berpengaruh nyata (p = 0,028) terhadap kadar zat besi roti tawar dan tidak berpengaruh nyata (p = 0,545) terhadap kadar serat pangan roti tawar.  Formula roti tawar yang terpilih berdasarkan kadar zat besi, serat pangan, dan uji organoleptik adalah F3 (50% tepung kedelai). Takaran saji roti tawar formula terpilih adalah 72 gram untuk 2 lembar dengan kandungan gizi sebesar 260,44 kkal energi total, 13,97 gr protein, 10,35 gr lemak, 27,83 gr karbohidrat, 3,33 mg zat besi, dan 34,22 gr serat pangan sehingga memenuhi klaim sebagai pangan dengan zat besi dan serat pangan.
Capaian Perkembangan Kognitif Anak Stunted dan Normal di TK/KB
Authors: Nirmala Besse
Published: July 2021

Anak  pendek  atau  stunting, merupakan  kondisi  gagal  tumbuh  pada anak  yang  terjadi sejak bayi dalam kandungan dan pada masa awal setelah  bayi  lahir. Akan  tetapi,  kondisi  stunting baru nampak setelah bayi berusia dua tahun akibat dari kekurangan gizi kronis sehingga anak terlalu pendek  untuk  usianya.  Kasus stunting ditemukan di Sulawesi Tengah khususnya di Kecamatan Palu Utara. Pendidikan Anak Usia Dini (PAUD) memiliki peran penting dalam berkontribusi mengantisipasi dan menurunkan  tingkat  stunting  pada anak. Data kesehatan anak pada lembaga PAUD, khususnya tinggi badan, berat badan dan lingkar kepala anak, umumnya hanya dicatat tanpa dianalisis maupun dimanfaatkan untuk dimensi layanan kesehatan dan gizi dalam program Holistik Integratif (HI). Keterlibatan PAUD menghadapi  fenomena  stunting  pada  anak di Indonesia dapat dilakukan melalui penerapan PAUD berbasis HI secara maksimal. Penelitian ini bertujuan untuk mengetahui capaian perkembangan kognitif pada anak yang stunting dan normal. Kriteria stunting ditentukan berdasarkan indeks TB/U <-2 SD. Penelitian ini melibatkan 134 anak TK di Kecamatan Palu Utara. Analisis data dilakukan melalui analisis univariat untuk melihat distribusi frekuensi dan bivariat untuk melihat hubungan antar variabel. Hasil penelitian menunjukkan bahwa terdapat 13 orang anak (9,7%) mengalami stunting dan terdapat 121 orang anak (91,3%) dalam kategori normal. Dilihat dari pencapaian perkembangan kognitif anak stunting, 5,2% belum berkembang dan 5,7% mulai berkembang. Berdasarkan hasil penelitian tersebut dapat disimpulkan bahwa ada hubungan antara kejadian stunting dengan capaian perkembangan kognitif anak usia dini.
Effect of Routine Thromboprophylaxis with Low Molecular Weight Heparin in Hospitalized Covid-19 Patients
Authors: Demir Tolga , Bitargil Macit , Kilic Helin El , Cetin Hakki Kursat , Bektas Nilufer , Kasapoglu Begum Ozuekren , Keskin Kudret , Sumerkan Mutlu Cagan , Ser Ozgur Selim , Demir Hale Bolgi , Balkaya Ahmet Ibrahim , Turkmen Ulku , Acar Sultan , Başı Nermin Balta , Sevgi Dilek Yildiz , Oncul Ahsen , Zengin Mehdi , Kabakci Birol , Alyan Ömer , Cinar Ayse Surhan , Dokmetas Ilyas , Koramaz Ismail , Ozdemir Haci Mustafa
Published: July 2021

Background: Coronavirus disease 2019 (COVID-19) is commonly complicated
with coagulopathy presented with venous thromboembolism and arterial
thromboses. The aim of this study was to evaluate the effect of routine
thromboprophylaxis with low molecular weight heparin (LMWH) on clinical
outcomes including mortality and need for intensive care unit (ICU)
admission in hospitalized COVID-19 patients. Methods: All confirmed
patients with COVID-19 hospitalized to COVID-19 dedicated wards, from
March 15 to May 15, 2020, were included in this retrospective cohort
study. Two groups of patients were established, according to the
non-routine and routine application of LMWH with therapeutic,
weight-based, anticoagulation dosing. Clinical, laboratory and treatment
data were collected, analyzed and compared between the two groups. A
logistic regression model was developed to assess the factors related to
in-hospital adverse outcomes. Results: A total of 1511 patients (797
men, median age 59.0 years) were retrospectively analyzed (Group
non-routine LMWH (n=828); group routine LMWH (n=683)). Multivariate
logistic regression analysis showed routine use of LMWH, favipiravir
administration, extreme values of WBC count, NLR, and troponin I as
factors independently associated with in-hospital adverse outcomes
(OR=0.25, 95% CI: 0.83-0.91; p<0.001 for routine use of
LMWH). Conclusion: Routine thromboprophylaxis with LMWH reduced
mortality and ICU admission in patients admitted with COVID-19
infection.
CONVOLUTION EQUATIONS ON THE ABELIAN GROUP $\cA(-1,1)$
Authors: Duduchava Roland
Published: July 2021

The interval $j=[-1,1]$ turns into an Abelian group
$\cA(\cJ)$ under the group operation
$x+_\cJ y:=(x+y)(1+xy)^{-1},\qquad
x,y\in\cJ$. This enables definition of
the invariant measure $d_\cJ x=(1-x^2)^{-1}dx$
and the Fourier transform $\cF_\cJ$ on
the interval $\cJ$ and, as a consequence, we can
consider Fourier convolution operators
$W^0_{\cJ,\cA}:=\cF_\cJ^{-1}\cA\cF_\cJ$
on $\cJ$. This class of convolutions includes
celebrated Prandtl, Tricomi and Lavrentjev-Bitsadze equations and, also,
differential equations of arbitrary order with the natural weighted
derivative $\fD_\cJ
u(x)=-(1-x^2)u’(x)$, $t\in\cJ$.
Equations are solved in the scale of Bessel potential
$\bH^s_p(\cJ,d_\cJ
x)$, $1\leqslant
p\leqslant\infty$, and
H\”older-Zygmound
$\bZ^\nu(\cJ,(1-x^2)^\mu)$,
$0<\mu,\nu<\infty$
spaces, adapted to the group $\cA(\cJ)$.
Boundedness of convolution operators (the problem of multipliers) is
discussed. The symbol $\cA(\xi)$,
$\xi\in\bR$, of a
convolution equation
$W^0_{\cJ,\cA}u=f$ defines
solvability: the equation is uniquely solvable if and only if the symbol
$\cA$ is elliptic. The solution is written explicitely
with the help of the inverse symbol. We touch shortly the
multidimensional analogue-the Abelian group
$\cA(\cJ^n)$.
Estudio fenológico de dos variedades de anturios, en el piedemonte llanero, en condiciones de vivero, para estudiantes de primer y séptimo semestre de Licenciatura en producción agropecuaria - Unillanos
Authors: Parrado Jhonny J. , García Gustavo A. , Vásquez Miguel Ángel , Rodríguez Mónica
Published: July 2021

Teniendo en cuenta que el piedemonte llanero cuenta con las condiciones de trópico húmedo ideales para el establecimiento del cultivo de anturios debido a que presenta las variables climáticas idóneas para su crecimiento. Este trabajo se realizó en la vereda Barcelona, a 9 km del casco urbano al sur oriente del municipio de Villavicencio específicamente en el Instituto de Investigaciones de la Orinoquia Colombiana (IIOC) de la Universidad de los Llanos ubicada a una altura de 400 m.s.n.m., con una temperatura promedio de 27,5 °C y una precipitación anual promedio de 2891 mm. Consideró como objetivo conocer lo aspectos de desarrollo y crecimiento fenológico de anturios para brindarle estos conocimientos y saberes para fortalecer el área de cultivo agrícolas y generar alternativas de producción. Se realizaron dos parcelas de 1.10 m de ancho y de largo 10 m para 140 plantas, divididos en 70 plantas de flor roja y 70 de flor blanca, se tomaron al azar 20 plantas de flor rojas y 20 plantas de flor blanca, las cuales fueron numeradas cada una. Después de establecida esta parcela se tomaron datos fenológicos cada 15 días en la cual se tomaron datos como altura de la planta, números de hojas, numero de flores. Para el análisis de resultados utilizó la prueba t de student. Se hicieron charlas y salidas a campo para obtener un mejor conocimiento. Una vez realizada la fase experimental y analizados los datos se procedió a desarrollar la fase pedagógica de la investigación, para ello se tomó como población los estudiantes de primer semestre de Licenciatura en Producción Agropecuaria de la Universidad de los Llanos. Se realizaron encuestas a los estudiantes de primer y séptimo La información obtenida, se dio a conocer por medio de una cartilla informativa, a productores y estudiantes de Lic. en producción agropecuaria. La evaluación fenológica del desarrollo y crecimiento de los anturios de acuerdo con la metodología implementada permitió determinar el establecimiento, manejo y variables de producción. La mayor producción de hojas y flores durante la evaluación fenológica se presenta en los Anturios Variedad album (Plantas blancas) entre 1 y 2 flores siempre. A diferencia de la producción de Anturios Variedad rubrum (Plantas rojas) que siempre mantuvieron de 1 y a veces 2 en menor proporción. No obstante, estas 2 variedades se pueden cultivar sin ningún problema. Durante el tiempo evaluado las dos variedades produjeron flores. Sin embargo, la variedad album obtuvo mejor respuesta a las condiciones del Piedemonte Llanero. De acuerdo a la encuesta aplicada a los estudiantes de primer semestre, en su mayoría, no conocen las ventajas en el manejo y explotación del cultivo de anturios, y de la alternativa de producción que pueden ser en el Departamento del Meta.
Melanoma maligno palpebral en un equino: reporte de un caso
Authors: Navarro Ortiz Cesar Augusto , Rodríguez Pineda Magda Elizabeth , Fuentes Reyes Edgar Edilberto
Published: July 2021

El objetivo de este trabajo fue relatar un caso de melanoma en un equino cruceto cuarto de milla, macho, de 10 años de edad, color moro, el cual fue llevado a la clínica de la Universidad de los Llanos por una masa en el parpado derecho que imposibilitaba su visión por dicho ojo, en Marzo del 2010. En este relato de caso, enfatizamos en signos clínicos; exámenes paraclínicos, tales como hemograma, biopsia excisional e histopatología, así como el tratamiento instaurado al animal.