bluuebunny/crossref_metadata_2025 huggingface dataset:
The Relationship between Prebiotic Supplementation and Anthropometric and Biochemical Parameters in Patients with NAFLD—A Systematic Review and Meta-Analysis of Randomized Controlled Trials
Authors: Stachowska Ewa , Portincasa Piero , Jamioł-Milc Dominika , Maciejewska-Markiewicz Dominika , Skonieczna-Żydecka Karolina
Published: November 2020

We aim to systematically review the efficacy of prebiotics in reducing anthropometric and biochemical parameters in individuals with non-alcoholic fatty liver disease (NAFLD). A systematic search using PubMed/MEDLINE, Embase, clinicaltrials.gov, Cinahl, and Web of Science of articles published up to 20 March 2020 was performed for randomized controlled trials enrolling >20 adult patients. Random-effect meta-analysis for metabolic outcomes in NAFLD patients was performed for anthropometric data in addition to liver enzyme, carbohydrate, and lipid parameters. We found six trials (comprising a total of 242 patients) with NAFLD, with subjects aged 38–52 years. The mean time of fiber administration varied between 10 and 12 weeks. The main fiber types were psyllium (seeds or powder), Ocimum basilicum (seeds), and high-performance inulin and oligofructose powder at doses of either 10 or 16 g per day. The control group received either maltodextrin (powder or capsules) or crushed wheat (powder). Patients on the diet with added fiber had improvements in body mass index (BMI) (standardized mean difference (SMD) = −0.494, 95% confidence interval (CI): −0.864 to −0.125, p = 0.009); alanine aminotransferase (ALT) (SMD = −0.667, 95% CI: −1.046 to −0.288, p = 0.001); aspartate aminotransferase (AST) (SMD = −0.466, 95% CI: −0.840 to −0.091, p = 0.015); fasting insulin (SMD = −0.705, 95% CI: −1.115 to −0.295, p = 0.001); and homeostasis model assessment for insulin resistance (HOMA-IR) (SMD = −0.619, 95% CI: −1.026 to −0.211, p = 0.003). Hence, the results show that fiber supplements result in favorable changes as reflected in the measurement of anthropometric, metabolic, and liver-related biomarkers, i.e., body mass index (BMI), homeostasis model assessment for insulin resistance (HOMA-IR), insulin, alanine aminotransferase (ALT), and aspartate aminotransferase (AST). These effects suggest the potential benefits of fiber consumption for NAFLD populations. More prospective, controlled studies should be conducted to reveal specific details regarding the fiber type, dosage, and duration for optimal intervention.
Sarcopenia Severity Based on Computed Tomography Image Analysis in Patients with Cirrhosis
Authors: Ebadi Maryam , Bhanji Rahima A. , Dunichand-Hoedl Abha R. , Mazurak Vera C. , Baracos Vickie E. , Montano-Loza Aldo J.
Published: November 2020

Standardized sex-specific cut-offs for sarcopenia in cirrhosis are needed to identify the risk of clinical complications and to discriminate the severity of sarcopenia. We aimed to compare clinical characteristics between patients with cirrhosis categorized according to the severity of sarcopenia. Computed tomography images were taken at the 3rd lumbar vertebra from 603 patients with cirrhosis and 129 adult donors for living liver transplantation. Patients with skeletal muscle index (SMI) two standard deviations (SD) below the sex-specific mean value of young donors (18–40 years old) were categorized as having severe sarcopenia whereas patients with SMI between −1 and −2 SD of the sex-specific young adult mean values were categorized as having sarcopenia. In the cirrhosis group, 408 patients (68%) were male with the mean age of 57 ± 0.4 years, and MELD score of 14 ± 0.4. Patients were divided into three groups: severe-sarcopenic (SMI < 30 cm2/m2 in females and <42 cm2/m2 in males), sarcopenic (30 ≤ SMI < 37 cm2/m2 in females and 42 ≤ SMI < 50 cm2/m2 in males) and non-sarcopenic (SMI ≥ 37 cm2/m2 in females and ≥50 cm2/m2 in males). Patients with cirrhosis and severe sarcopenia had lower muscle radiodensity and higher plasma neutrophil as well as neutrophil to lymphocyte ratio levels than both non- and sarcopenic groups. The frequency of alcohol-induced cirrhosis, refractory ascites, hepatic encephalopathy, CRP > 20 mg/mL, and severe malnutrition was also higher in severe-sarcopenic patients. The interval between sarcopenia and severe sarcopenia may reflect a window of opportunity in which to intervene and mitigate muscle wasting to improve patient outcomes.
Multiple Micronutrient Supplementation during Pregnancy and Increased Birth Weight and Skinfold Thicknesses in the Offspring: The Cambridge Baby Growth Study
Authors: Petry Clive J. , Ong Ken K. , Hughes Ieuan A. , Dunger David B.
Published: November 2020

Multiple micronutrient supplementation (MMS) in pregnancy has previously been associated with positive effects on fetal growth, but its value in high-income countries remains controversial. In this study, we investigated effects of pregnancy MMS on offspring size at birth and adiposity, along with risks of various maternal outcomes of pregnancy, using the prospective Cambridge Baby Growth Study. Maternal MMS was reported in 528 out of 970 women who completed pregnancy questionnaires. Gestational diabetes (GDM) was assessed using results from 75 g oral glucose tolerance tests at week 28 of pregnancy. Offspring size at birth was assessed using standard anthropometric measurements and adiposity using skinfold calipers. MMS was associated with increased risk of developing GDM (risk ratio = 1.86 (1.13–3.08), p = 0.02), as well as increased offspring size at birth in terms of weight (p = 0.03), head circumference (p = 0.04), and flank, and subscapular and triceps skinfold thicknesses (p = 0.04, 0.03, and 0.003, respectively). There was no association with quadriceps skinfold thickness (p = 0.2), suggesting that the increased adiposity was partially regionalized. In women who underwent oral glucose tolerance testing, nearly all of these associations were attenuated by adjusting for GDM. These results suggest that the increased offspring size at birth, including (regionalized) adiposity associated with pregnancy, and MMS may be partially related to the development of GDM.
Impact of Porcine Arterivirus, Influenza B, and Their Coinfection on Antiviral Response in the Porcine Lung
Authors: Fleming Damarius S. , Miller Laura C. , Tian Yun , Li Yonghai , Ma Wenjun , Sang Yongming
Published: November 2020

Interferon (IFN) cytokines induce an autonomous antiviral state in cells of the infected site to restrict virus spreading and critically regulate overall antiviral response. The antiviral state leads to host protection through expression of hundreds of IFN-stimulated genes that restrict viral infection through multiple mechanisms, for example, directly in viral genome degradation and indirectly through cellular metabolic inhibition. Young pigs were split into four treatment groups: control, porcine reproductive and respiratory syndrome virus (PRRSV, also known as porcine arterivirus) infected, influenza B virus (IBV) infected, and IBV/PRRSV coinfection. Lung tissue was collected at 3, 5, and 7 days post infection (dpi) for control, PRRSV and IBV/PRRSV coinfection, and at 3 and 5 dpi for IBV. Transcriptomic analysis, using usegalaxy.org tools, was performed against the S.scrofa 11.1 reference genome. Differentially expressed gene (DEG) analysis was carried out using DeSeq2 based on the model treatment + dpi + treatment:dpi + E. Downstream analysis examined the interaction of DEG at each dpi for over-enriched gene ontology (G.O.) terms and pathways. Comparisons of the infected groups vs. the controls yielded a total of (n = 1412) DEGs for the PRRSV group and (n = 1578) for the IBV/PRRSV group across all timepoints. The IBV group had (n = 64) total DEGs across 3 and 5 dpi. Expression data were considered statistically significant based on false discovery rate (FDR) ⫹ 0.1. Venn diagram comparisons of the DEGs across dpi showed that groups shared only 16 DEGs at 3 dpi, no DEGs were shared at 5 dpi, and for 7 dpi, only the PRRSV and IBV/PRRSV groups were compared and shared a total of 43 DEGs. Across the comparisons, differential expression was observed in antiviral genes such as IRF1, MX1, and OAS2. The IBV and IBV/PRRSV groups showed higher expression of antiviral genes at earlier dpi than the PRRSV group. Additionally, downregulated genes from the comparisons clustered around Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways effecting lung development and cellular integrity. Early expression of host IFN and antiviral genes may lead to viral RNA degradation, and assembly and transcription inhibition in the IBV infections. In comparison, expression of antiviral genes in the PRRSV group decreased across time. The decrease may explain why PRRSV infections persist, while IBV clears. Moreover, all infected groups showed prolonged upregulation in neutrophil degranulation pathway activity, possibly exacerbating symptomatic lung lesion pathology seen in these respiratory infections.
Systematic Review of Ticks and Tick-Borne Pathogens of Small Ruminants in Pakistan
Authors: Ghafar Abdul , Abbas Tariq , Rehman Abdul , Sandhu Zia-ud-Din , Cabezas-Cruz Alejandro , Jabbar Abdul
Published: November 2020

Ticks and tick-borne diseases (TTBDis) are a major constraint to the health and production of small ruminants in Pakistan. Despite being the subject of intermittent studies over the past few decades, comprehensive information on the epidemiology and control of TTBDis is lacking. Herein, we have systematically reviewed the current knowledge on TTBDis of small ruminants in Pakistan. Critical appraisal of the selected 71 articles published between 1947 to 2020 revealed that morphological examination had been the most widely used method for the identification of TTBDis in Pakistan. Tick fauna comprise at least 40 species, mainly belonging to Haemaphysalis, Hyalomma and Rhipicephalus. The prevalence of ticks is the highest in summer (June–September) and it is also higher in goats than sheep. Anaplasma, Babesia and Theileria spp. are the major tick-borne pathogens (TBPs), and their prevalence is usually higher in sheep than goats. Spatio-temporal distribution, genetic diversity and control of ticks and TBPs of small ruminants as well as the competence of tick vectors for various TBPs remain to be explored. Therefore, coordinated and focused investigations are required to fill knowledge gaps in these areas to maximise the health, production and welfare of small ruminants and minimise economic losses associated with TTBDis in Pakistan.
Germinated Soybean Embryo Extract Ameliorates Fatty Liver Injury in High-Fat Diet-Fed Obese Mice
Authors: Kwon Doyoung , Kim Sou Hyun , Son Seung Won , Seo Jinuk , Jeong Tae Bin , Kim Kyung-Mi , Jung Jae-Chul , Jung Mi Sook , Lee Yun-Hee , Jung Young-Suk
Published: November 2020

Soybean is known to have diverse beneficial effects against human diseases, including obesity and its related metabolic disorders. Germinated soybean embryos are enriched with bioactive phytochemicals and known to inhibit diet-induced obesity in mice, but their effect on non-alcoholic fatty liver disease (NAFLD) remains unknown. Here, we germinated soybean embryos for 24 h, and their ethanolic extract (GSEE, 15 and 45 mg/kg) was administered daily to mice fed with a high-fat diet (HFD) for 10 weeks. HFD significantly increased the weight of the body, liver and adipose tissue, as well as serum lipid markers, but soyasaponin Ab-rich GSEE alleviated these changes. Hepatic injury and triglyceride accumulation in HFD-fed mice were attenuated by GSEE via decreased lipid synthesis (SREBP1c) and increased fatty acid oxidation (p-AMPKα, PPARα, PGC1α, and ACOX) and lipid export (MTTP and ApoB). HFD-induced inflammation (TNF-α, IL-6, IL-1β, CD14, F4/80, iNOS, and COX2) was normalized by GSEE in mice livers. In adipose tissue, GSEE downregulated white adipose tissue (WAT) differentiation and lipogenesis (PPARγ, C/EBPα, and FAS) and induced browning genes (PGC1α, PRDM16, CIDEA, and UCP1), which could also beneficially affect the liver via lowering adipose tissue-related circulating lipid levels. Thus, our results suggest that GSEE can prevent HFD-induced NAFLD via inhibition of hepatic inflammation and restoration of lipid metabolisms in both liver and adipose tissue.
Preparation and Characterization of Solid Dispersions Composed of Curcumin, Hydroxypropyl Cellulose and/or Sodium Dodecyl Sulfate by Grinding with Vibrational Ball Milling
Authors: Mai Nguyen Ngoc Sao , Otsuka Yuta , Kawano Yayoi , Hanawa Takehisa
Published: November 2020

Solubility is an important physicochemical property affecting drug bioavailability. One approach to improve drug solubility is using amorphous formulations, which can improve solubility by up to a 1000-fold. Herein, amorphous curcumin (CUR) and amorphous solid dispersions (SDs) consisting of CUR, hydroxypropyl cellulose (HPC) and/or sodium dodecyl sulfate (SDS) were developed using vibrational ball milling. The resulting ground mixtures (GMs) were characterized using powder X-ray diffractometry, Fourier transform infrared spectroscopy, differential scanning calorimetry and a dissolution test. The 60-min GM containing 90% HPC significantly increased the drug solubility. Presence of SDS in the GMs containing 90% HPC reduced the grinding duration from 60 min to 30 min in forming a ground SD that significantly increased the CUR dissolution rate. This amorphous state was stable for 30 days when stored at 40 °C/RH 75%.
Supramolecular Caffeic Acid and Bortezomib Nanomedicine: Prodrug Inducing Reactive Oxygen Species and Inhibiting Cancer Cell Survival
Authors: Aguilar Ludwig Erik , Jang Se Rim , Park Chan Hee , Lee Kang Min
Published: November 2020

Phenolics from plant materials have garnered attention in nanomedicine research, due to their various medicinal properties. Caffeic acid, a phenolic compound that is abundant in coffee beans, has been proven to have anticancer effects, due to its reactive oxygen species (ROS)-inducing properties. Here, a supramolecular nanomedicine was designed using caffeic acid molecule and the synthetic anticancer drug bortezomib, via catechol–boronic acid conjugation and Fe(III) ion crosslinking. Bortezomib is a proteasome-inhibiting drug and its boronic acid functional group can bind to caffeic acid’s catechol moiety. By having a nanoparticle formulation that can deliver bortezomib via intracellular endocytosis, the catechol–boronic acid conjugation can be dissociated, which liberates the boronic acid functional group to bind to the 26S proteasome of the cell. The ROS-inducing property of caffeic acid also complements the bortezomib payload, as the latter suppresses the survival mechanism of the cell through NF-κB inhibition.
Therapeutic Approaches to Treat Mitochondrial Diseases: “One-Size-Fits-All” and “Precision Medicine” Strategies
Authors: Bottani Emanuela , Lamperti Costanza , Prigione Alessandro , Tiranti Valeria , Persico Nicola , Brunetti Dario
Published: November 2020

Primary mitochondrial diseases (PMD) refer to a group of severe, often inherited genetic conditions due to mutations in the mitochondrial genome or in the nuclear genes encoding for proteins involved in oxidative phosphorylation (OXPHOS). The mutations hamper the last step of aerobic metabolism, affecting the primary source of cellular ATP synthesis. Mitochondrial diseases are characterized by extremely heterogeneous symptoms, ranging from organ-specific to multisystemic dysfunction with different clinical courses. The limited information of the natural history, the limitations of currently available preclinical models, coupled with the large variability of phenotypical presentations of PMD patients, have strongly penalized the development of effective therapies. However, new therapeutic strategies have been emerging, often with promising preclinical and clinical results. Here we review the state of the art on experimental treatments for mitochondrial diseases, presenting “one-size-fits-all” approaches and precision medicine strategies. Finally, we propose novel perspective therapeutic plans, either based on preclinical studies or currently used for other genetic or metabolic diseases that could be transferred to PMD.
A Computational Study on Performance Improvement of THz Signal from a Grating Photoconductive Antenna
Authors: Chia Jia Yi , Tantiwanichapan Khwanchai , Jintamethasawat Rungroj , Sathukarn Asmar , Kusolthossakul Woraprach , Nuntawong Noppadon
Published: November 2020

A diffractive grating is a well-known optical component and is extensively used in many applications. This research work explores application of the diffractive grating in a photoconductive antenna (PCA) of a terahertz time domain spectroscopy (THz-TDS) system, by utilizing benefits of a sub-wavelength grating structure. The grating PCA structure was modeled and simulated by COMSOL Multiphysics software (COMSOL, Inc., Burlington, MA, USA). Performance of the proposed PCA design is studied in terms of its induced photocurrent. The effects of geometrical parameters of the grating are also investigated and analyzed through its optical and electrical responses. Thanks to the increase in absorption of the incident laser’s electric field, the simulation results show a 63% increment of the induced photocurrent in the grating PCA, compared with the conventional planar PCA.
Use of LAMP for Assessing Botrytis cinerea Colonization of Bunch Trash and Latent Infection of Berries in Grapevines
Authors: Si Ammour Melissa , Castaldo Eleonora , Fedele Giorgia , Rossi Vittorio
Published: November 2020

A real-time loop-mediated isothermal amplification (LAMP) assay was evaluated for the detection of Botrytis cinerea in grapevine bunch trash, immature berries, and ripening berries. A simple method for the preparation of crude extracts of grape tissue was also developed for on-site LAMP analysis. When tested with 14 other fungal species frequently found in grapevines, the LAMP assay was specific and sensitive to a B. cinerea DNA quantity of 0.1 ng/µL. The sensitivity was further tested using bunch trash samples with B. cinerea colonization levels between 6 and 100% and with bulk-berry samples composed of 4 pathogen-free berries or 4 berries among which 25 to 100% had been inoculated with B. cinerea. The LAMP assay detected the lowest B. cinerea colonization level tested in bunch trash and in immature and mature berries in less than 20 min. In single-berry experiments, LAMP amplified B. cinerea DNA from all artificially inoculated individual immature and mature berries. No amplification occurred in B. cinerea-free material. The real-time LAMP assay has the potential to be used as a rapid on-site diagnostic tool for assessing B. cinerea colonization in bunch trash and B. cinerea latent infections in berries, which represent critical stages for decision-making about disease management.
In Silico Identification of MYB and bHLH Families Reveals Candidate Transcription Factors for Secondary Metabolic Pathways in Cannabis sativa L.
Authors: Bassolino Laura , Buti Matteo , Fulvio Flavia , Pennesi Alessandro , Mandolino Giuseppe , Milc Justyna , Francia Enrico , Paris Roberta
Published: November 2020

Plant secondary metabolic pathways are finely regulated by the activity of transcription factors, among which members of the bHLH and MYB subfamilies play a main role. Cannabis sativa L. is a unique officinal plant species with over 600 synthesized phytochemicals having diverse scale-up industrial and pharmaceutical usage. Despite comprehensive knowledge of cannabinoids’ metabolic pathways, very little is known about their regulation, while the literature on flavonoids’ metabolic pathways is still scarce. In this study, we provide the first genome-wide analysis of bHLH and MYB families in C. sativa reference cultivar CBDRx and identification of candidate coding sequences for these transcription factors. Cannabis sativa bHLHs and MYBs were then classified into functional subfamilies through comparative phylogenetic analysis with A. thaliana transcription factors. Analyses of gene structure and motif distribution confirmed that CsbHLHs and CsMYBs belonging to the same evolutionary clade share common features at both gene and amino acidic level. Candidate regulatory genes for key metabolic pathways leading to flavonoid and cannabinoid synthesis in Cannabis were also retrieved. Furthermore, a candidate gene approach was used to identify structural enzyme-coding genes for flavonoid and cannabinoid synthesis. Taken as a whole, this work represents a valuable resource of candidate genes for further investigation of the C. sativa cannabinoid and flavonoid metabolic pathways for genomic studies and breeding programs.
Genetic Variation in Damaged Populations of Pistacia atlantica Desf.
Authors: Labdelli Amina , De La Herrán Roberto , Arafeh Rami , Resentini Francesca , Trainotti Livio , Halis Youcef , Adda Ahmed , Merah Othmane
Published: November 2020

The Atlas Pistachio tree, Pistacia atlantica Desf., has great importance in the ecological landscape of North Africa, due to its adaptive plasticity, as well as its use as a rootstock in the cultivation of the economically important species, Pistacia vera L. The conservation and valuation of this species require sampling and an assessment of its genetic variability. For the first time in North Africa, the inter-simple sequence repeats (ISSR) molecular marker has been used in genetic-diversity assessment and in the population relationships of P. atlantica subsp. atlantica. The ISSR markers tested showed 74.1% polymorphism, while molecular variance (AMOVA) analysis revealed a high percentage of the total genetic diversity of 55.7% among the four populations studied. Cluster analysis with neighbor-joining (NJ) and principal coordinate analysis (PCO) divided the study sites into four distinct groups according to their geographical locations (Tiaret, Batna, Djelfa, and Bechar). Isolation by distance or Mantel test gave a positive correlation of r = 0.86 between geographical and genetic distances. The results in this study indicate an absence of gene flow, implying that conservation efforts should be taken separately for each population.
Light Quality Affected the Growth and Root Organic Carbon and Autotoxin Secretions of Hydroponic Lettuce
Authors: Zhou Chengbo , Zhang Yubin , Liu Wenke , Zha Lingyan , Shao Mingjie , Li Baoshi
Published: November 2020

Light is a crucial environmental signal and photosynthetic energy for plant growth, development, and primary and secondary metabolism. To explore the effects of light quality on the growth and root exudates of hydroponic lettuce (Lactuca sativa L.), white LED (W, control) and four the mixtures of red (R) and blue (B) LED with different R/B light intensity ratios (R/B = 2, 2R1B; R/B = 3, 3R1B; R/B = 4, 4R1B; and R/B = 8, 8R1B) were designed. The results showed that the biomass of lettuce under 8R1B and W treatments was higher than that under other light quality treatments. The photosynthetic rate (Pn) under red and blue light was significantly higher than that of white light. Total root length, root surface area, and root volume were the highest under 8R1B. 4R1B treatment significant increased root activity by 68.6% compared with W. In addition, total organic carbon (TOC) content, TOC content/shoot dry weight, TOC content/root dry weight, and TOC content/root surface area were the highest under 4R1B. Moreover, 8R1B treatment reduced the concentration of benzoic acid and salicylic acid, and the secretion ability of benzoic acid and salicylic acid by per unit root surface area and accumulation by per unit shoot dry weight. In addition, 2R1B and 3R1B reduced the secretion ability of gallic acid and tannic acid by per unit root surface area and accumulation by per unit shoot dry weight. In conclusion, this study showed that the secretion of autotoxins could be reduced through the mediation of red and blue light composition of LEDs in a plant factory. In terms of autotoxin secretion reduction efficiency and yield performance of lettuce, 8R1B light regime is recommended for practical use.
Turfgrass Disease Diagnosis: Past, Present, and Future
Authors: Stackhouse Tammy , Martinez-Espinoza Alfredo D. , Ali Md Emran
Published: November 2020

Turfgrass is a multibillion-dollar industry severely affected by plant pathogens including fungi, bacteria, viruses, and nematodes. Many of the diseases in turfgrass have similar signs and symptoms, making it difficult to diagnose the specific problem pathogen. Incorrect diagnosis leads to the delay of treatment and excessive use of chemicals. To effectively control these diseases, it is important to have rapid and accurate detection systems in the early stages of infection that harbor relatively low pathogen populations. There are many methods for diagnosing pathogens on turfgrass. Traditional methods include symptoms, morphology, and microscopy identification. These have been followed by nucleic acid detection and onsite detection techniques. Many of these methods allow for rapid diagnosis, some even within the field without much expertise. There are several methods that have great potential, such as high-throughput sequencing and remote sensing. Utilization of these techniques for disease diagnosis allows for faster and accurate disease diagnosis and a reduction in damage and cost of control. Understanding of each of these techniques can allow researchers to select which method is best suited for their pathogen of interest. The objective of this article is to provide an overview of the turfgrass diagnostics efforts used and highlight prospects for disease detection.
Some Mechanisms Modulating the Root Growth of Various Wheat Species under Osmotic-Stress Conditions
Authors: Terletskaya Nina V. , Lee Tamara E. , Altayeva Nazira A. , Kudrina Nataliya O. , Blavachinskaya Irina V. , Erezhetova Ulzhan
Published: November 2020

The role of the root in water supply and plant viability is especially important if plants are subjected to stress at the juvenile stage. This article describes the study of morphophysiological and cytological responses, as well as elements of the anatomical structure of primary roots of three wheat species, Triticum monococcum L., Triticum dicoccum Shuebl., and Triticum aestivum L., to osmotic stress. It was shown that the degree of plasticity of root morphology in water deficit affected the growth and development of aboveground organs. It was found that in conditions of osmotic stress, the anatomical root modulations were species-specific. In control conditions the increase in absolute values of root diameter was reduced with the increase in the ploidy of wheat species. Species-specific cytological responses to water deficit of apical meristem cells were also shown. The development of plasmolysis, interpreted as a symptom of reduced viability apical meristem cells, was revealed. A significant increase in enzymatic activity of superoxide dismutase under osmotic stress was found to be one of the mechanisms that could facilitate root elongation in adverse conditions. The tetraploid species T. dicoccum Shuebl. were confirmed as a source of traits of drought tolerant primary root system for crosses with wheat cultivars.
Effects of Topological Constraints on Penetration Structures of Semi-Flexible Ring Polymers
Authors: Guo Fuchen , Li Ke , Wu Jiaxin , He Linli , Zhang Linxi
Published: November 2020

The effects of topological constraints on penetration structures of semi-flexible ring polymers in a melt are investigated using molecular dynamics simulations, considering simultaneously the effects of the chain stiffness. Three topology types of rings are considered: 01-knot (the unknotted), 31-knot and 61-knot ring polymers, respectively. With the improved algorithm to detect and quantify the inter-ring penetration (or inter-ring threading), the degree of ring threading does not increase monotonously with the chain stiffness, existing a peak value at the intermediate stiffness. It indicates that rings interpenetrate most at intermediate stiffness where there is a balance between coil expansion (favoring penetrations) and stiffness (inhibiting penetrations). Meanwhile, the inter-ring penetration would be suppressed with the knot complexity of the rings. The analysis of effective potential between the rings provides a better understanding for this non-monotonous behavior in inter-ring penetration.
Comparison of Techniques to Control Ice Nucleation during Lyophilization
Authors: Luoma Jacob , Ingham Erika , Lema Martinez Carmen , Allmendinger Andrea
Published: November 2020

Controlling ice nucleation during lyophilization of parenteral drug products increases the homogeneity of critical quality attributes, such as residual moisture, across drug product batches and shortens lyophilization cycle time. In the present study, we compare three mechanistically different techniques to control ice nucleation during the freezing step of lyophilization, which are referred to as “depressurization”, “partial vacuum”, and “ice fog” techniques. The techniques are compared with respect to their operational limitations and challenges. Installation considerations are also discussed. Using the aforementioned nucleation techniques, we investigated a monoclonal antibody formulation and an enzyme formulation at different protein concentrations using feasible nucleation temperatures and different vial formats and fill volumes. Samples were compared for solid state properties and other critical quality attributes on stability. When nucleated at the same temperature, the three techniques produced products with the same quality attributes and stability behavior. Under conditions resulting in micro-collapse, stability behavior can be different. We found that each technology had considerations for achieving robust nucleation. The present comparison may serve as guidance in selecting a nucleation method.
A Charge-Based Mechanistic Study into the Effects of Process Parameters on Fiber Accumulating Geometry for a Melt Electrohydrodynamic Process
Authors: Cao Kai , Zhang Fucheng , Chang Robert C.
Published: November 2020

Melt electrohydrodynamic processes, in conjunction with a moveable collector, have promising engineered tissue applications. However, the residual charges within the fibers deteriorate its printing fidelity. To clarify the mechanism through which the residual charges play roles and exclude the confounding effects of collector movement, a stationary printing mode is adopted in which fibers deposit on a stationary collector. Effects of process parameters on generalizable printing outcomes are studied herein. The fiber deposit bears a unique shape signature typified by a central cone surrounded by an outer ring and is characterized by a ratio of its height and base diameter Hdep/Ddep. Results indicate Hdep/Ddep increases with collector temperature and decreases slightly with voltage. Moreover, the steady-state dynamic jet deposition process is recorded and analyzed at different collector temperatures. A charge-based polarization mechanism describing the effect of collector temperature on the fiber accumulating shape is apparent in both initial and steady-state phases of fiber deposition. Therefore, a key outcome of this study is the identification and mechanistic understanding of collector temperature as a tunable process variable that can yield predictable structural outcomes. This may have cross-cutting potential for additive manufacturing process applications such as the melt electrowriting of layered scaffolds.
Ultrasound Assisted Extraction of Phenolic Compounds from a Jujube By-Product with Valuable Bioactivities
Authors: Berkani Farida , Serralheiro Maria Luisa , Dahmoune Farid , Ressaissi Asma , Kadri Nabil , Remini Hocine
Published: November 2020

Jujube plant is a potential source of polyphenols with biological propreties. The purpose of this study was to investigate the application of ultrasound technique for extracting phenolic compounds (TPC) from seeds of Zizyphus lotus under optimization conditions based on response surface methodology. A maximum TPC, total flavonoids content (TFC), and total condensed tannins content (TTC) of 2383.10 ± 0.87 mg GAE/100g, 486.50 ± 0.38 mg QE/100g and 15,787.10 ± 0.10 mg CE/100g, respectively obtained under ethanol concentration 50.16%, sonication temperature 29.01 °C, sonication time 15.94 min and solvent-to-solid ratio 34.10:1 mL/g. The optimized extract was then evaluated for its antioxidant, antiacethylcholinesterase, antihypercholesterolemia, and antiproliferative activities. The results showed that ultrasound method is a green and safe method that can be used to effectively extract TPC from jujube seeds. The biological activity of Zizyphus extract exhibited a very good antioxidant against DPPH (EC50 = 0.39 µg/mL) and FRAP (1670.42 ± 6.5 mg/100 g). Additionally, it possesses acetylcholinesterase (AChE) inhibitory effect (IC50 = 0.93 ± 0.01 mg/mL) and HMGR inhibition (45.41%) using 100 µg/mL. The extract significantly inhibits cell proliferation on the MCF-7 and HepG2 tumor cell lines with an IC50 values of <0.05 and 3 ± 0.55 mg/mL, respectively. Therefore, the ultrasound method can be considered a method for obtaining a significant anticancer activity with respect to the lines and therefore makes it possible to recover a maximum of phenolic compounds in less time with an AChE and HMGR inhibitory activity. Thus, it can be suggested that Zls extract is a promising fruit for the development of supplementary dietary due to its potential behaviour as nutraceutical.
Identifying Potential Mosquito Breeding Grounds: Assessing the Efficiency of UAV Technology in Public Health
Authors: Schenkel Jared , Taele Paul , Goldberg Daniel , Horney Jennifer , Hammond Tracy
Published: November 2020

Human ecology has played an essential role in the spread of mosquito-borne diseases. With standing water as a significant factor contributing to mosquito breeding, artificial containers disposed of as trash—which are capable of holding standing water—provide suitable environments for mosquito larvae to develop. The development of these larvae further contributes to the possibility for local transmission of mosquito-borne diseases in urban areas such as Zika virus. One potential solution to address this issue involves leveraging unmanned aerial vehicles that are already systematically becoming more utilized in the field of geospatial technology. With higher pixel resolution in comparison to satellite imagery, as well as having the ability to update spatial data more frequently, we are interested in investigating the feasibility of unmanned aerial vehicles as a potential technology for efficiently mapping potential breeding grounds. Therefore, we conducted a comparative study that evaluated the performance of an unmanned aerial vehicle for identifying artificial containers to that of conventionally utilized GPS receivers. The study was designed to better inform researchers on the current viability of such devices for locating a potential factor (i.e., small form factor artificial containers that can host mosquito breeding grounds) in the local transmission of mosquito-borne diseases. By assessing the performance of an unmanned aerial vehicle against ground-truth global position system technology, we can determine the effectiveness of unmanned aerial vehicles on this problem through our selected metrics of: timeliness, sensitivity, and specificity. For the study, we investigated these effectiveness metrics between the two technologies of interest in surveying a study area: unmanned aerial vehicles (i.e., DJI Phantom 3 Standard) and global position system-based receivers (i.e., Garmin GPSMAP 76Cx and the Garmin GPSMAP 78). We first conducted a design study with nine external participants, who collected 678 waypoint data and 214 aerial images from commercial GPS receivers and UAV, respectively. The participants then processed these data with professional mapping software for visually identifying and spatially marking artificial containers between the aerial imagery and the ground truth GPS data, respectively. From applying statistical methods (i.e., two-tailed, paired t-test) on the participants’ data for comparing how the two technologies performed against each other, our data analysis revealed that the GPS method performed better than the UAV method for the study task of identifying the target small form factor artificial containers.
A Semi-Automated Method for Estimating Adélie Penguin Colony Abundance from a Fusion of Multispectral and Thermal Imagery Collected with Unoccupied Aircraft Systems
Authors: Bird Clara N. , Dawn Allison H. , Dale Julian , Johnston David W.
Published: November 2020

Monitoring Adélie penguin (Pygoscelis adeliae) populations on the Western Antarctic Peninsula (WAP) provides information about the health of the species and the WAP marine ecosystem itself. In January 2017, surveys of Adélie penguin colonies at Avian Island and Torgersen Island off the WAP were conducted via unoccupied aircraft systems (UAS) collecting optical Red Green Blue (RGB), thermal, and multispectral imagery. A semi-automated workflow to count individual penguins using a fusion of multispectral and thermal imagery was developed and combined into an ArcGIS workflow. This workflow isolates colonies using multispectral imagery and detects and counts individuals by thermal signatures. Two analysts conducted manual counts from synoptic RGB UAS imagery. The automated system deviated from analyst counts by −3.96% on Avian Island and by 17.83% on Torgersen Island. However, colony-by-colony comparisons revealed that the greatest deviations occurred at larger colonies. Matched pairs analysis revealed no significant differences between automated and manual counts at both locations (p > 0.31) and linear regressions of colony sizes from both methods revealed significant positive relationships approaching unity (p < 0.0002. R2 = 0.91). These results indicate that combining UAS surveys with sensor fusion techniques and semi-automated workflows provide efficient and accurate methods for monitoring seabird colonies in remote environments.
DInSAR for Road Infrastructure Monitoring: Case Study Highway Network of Rome Metropolitan (Italy)
Authors: Orellana Felipe , Delgado Blasco Jose Manuel , Foumelis Michael , D’Aranno Peppe J.V. , Marsella Maria A. , Di Mascio Paola
Published: November 2020

The road network of metropolitan Rome is determined by a large number of structures located in different geological environments. To maintain security and service conditions, satellite-based monitoring can play a key role, since it can cover large areas by accurately detecting ground displacements due to anthropic activities (underground excavations, interference with other infrastructures, etc.) or natural hazards, mainly connected to the critical hydrogeological events. To investigate the area, two different Differential Interferometry Synthetic Aperture Radar (DInSAR) processing methods were used in this study: the first with open source using the Persistent Scatterers Interferometry (PSI) of SNAP-StaMPS workflow for Sentinel-1 (SNT1) and the second with the SBAS technique for Cosmo-SkyMed (CSK). The results obtained can corroborate the displacement trends due to the characteristics of the soil and the geological environments. With Sentinel-1 data, we were able to obtain the general deformation overview of the overall highways network, followed by a selection and classification of the PSI content for each section. With Cosmo-SkyMed data, we were able to increase the precision in the analysis for one sample infrastructure for which high-resolution data from CSK were available. Both datasets were demonstrated to be valuable for collecting data useful to understand the safety condition of the infrastructure and to support the maintenance actions.
Vis-NIR Spectroscopy and Satellite Landsat-8 OLI Data to Map Soil Nutrients in Arid Conditions: A Case Study of the Northwest Coast of Egypt
Authors: Mohamed Elsayed Said , Baroudy A. A El , El-beshbeshy T. , Emam M. , Belal A. A. , Elfadaly Abdelaziz , Aldosari Ali A. , Ali Abdelraouf. M. , Lasaponara Rosa
Published: November 2020

The mapping of soil nutrients is a key issue for numerous applications and research fields ranging from global changes to environmental degradation, from sustainable soil management to the precision agriculture concept. The characterization, modeling and mapping of soil properties at diverse spatial and temporal scales are key factors required for different environments. This paper is focused on the use and comparison of soil chemical analyses, Visible near infrared and shortwave infrared VNIR-SWIR spectroscopy, partial least-squares regression (PLSR), Ordinary Kriging (OK), and Landsat-8 operational land imager (OLI) images, to inexpensively analyze and predict the content of different soil nutrients (nitrogen (N), phosphorus (P), and potassium (K)), pH, and soil organic matter (SOM) in arid conditions. To achieve this aim, 100 surface samples of soil were gathered to a depth of 25 cm in the Wadi El-Garawla area (the northwest coast of Egypt) using chemical analyses and reflectance spectroscopy in the wavelength range from 350 to 2500 nm. PLSR was used firstly to model the relationship between the averaged values from the ASD spectroradiometer and the available N, P, and K, pH and SOM contents in soils in order to map the predicted value using Ordinary Kriging (OK) and secondly to retrieve N, P, K, pH, and SOM values from OLI images. Thirty soil samples were selected to verify the validity of the results. The randomly selected samples included the spatial diversity and characteristics of the study area. The prediction of available of N, P, K pH and SOM in soils using VNIR-SWIR spectroscopy showed high performance (where R2 was 0.89, 0.72, 0.91, 0.65, and 0.75, respectively) and quite satisfactory results from Landsat-8 OLI images (correlation R2 values 0.71, 0.68, 0.55, 0.62 and 0.7, respectively). The results showed that about 84% of the soils of Wadi El-Garawla are characterized by low-to-moderate fertility, while about 16% of the area is characterized by high soil fertility.
A New Method for Automated Clearcut Disturbance Detection in Mediterranean Coppice Forests Using Landsat Time Series
Authors: Giannetti Francesca , Pegna Raffaello , Francini Saverio , McRoberts Ronald E. , Travaglini Davide , Marchetti Marco , Scarascia Mugnozza Giuseppe , Chirici Gherardo
Published: November 2020

A Landsat time series has been recognized as a viable source of information for monitoring and assessing forest disturbances and for continuous reporting on forest dynamics. This study focused on developing automated procedures for detecting disturbances in Mediterranean coppice forests which are characterized by rapid regrowth after a cut. Specifically, new methods specific to Mediterranean coppice forests are needed for mapping clearcut disturbances over time and for estimating related indicators in the context of Sustainable Forest Management and Biodiversity International monitoring frameworks. The aim of this work was to develop a new change detection algorithm for mapping clearcut disturbances in Mediterranean coppice forests with Landsat time series (LTS) using a short time window. Accuracy for the new algorithm, characterized as the Two Thresholds Method (TTM), was evaluated using an independent clearcut reference dataset over a temporal period of the 13 years between 2001 and 2013. TTM was also evaluated against two benchmark approaches: (i) LandTrendr, and (ii) the forest loss category of the Global Forest Change Map. Overall Accuracy for LandTrendr and TTM were greater than 0.94. Meanwhile, smaller accuracies were always obtained for the GFC. In particular, Producer’s Accuracy ranged between 0.45 and 0.84 for TTM and between 0.49 and 0.83 for LT, while for the GFC, PA ranged between 0 and 0.38. User’s Accuracy ranged between 0.86 and 0.96 for TTM and between 0.73 and 0.91 for LT, while for the GFC UA ranged between 0.19 and 1.00. Moreover, to illustrate the utility of TTM for mapping clearcut disturbances in Mediterranean coppice forests, we applied TTM to a Landsat scene that covered almost the entirety of the Tuscany region in Italy.
The Social Network Consequences of a Gang Murder Blowout
Authors: Airola Alice , Bouchard Martin
Published: November 2020

An unexpected crisis in a criminal organization offers a rare opportunity to analyze whether and how the configuration of business and trust relationships changes in response to external shocks. The current study recreates the social network of the Red Scorpion gang members involved in the Surrey Six Murder, one of the deadliest gang-related homicides to occur in Canada. The event, which involved two bystanders and six victims in total, was the result of a poorly executed retaliation. Our analyses focus on two phases of the network, the conspiracy phase and the post-murder phase. In each phase, we examine the balance of business, trust, and conflictual ties. Results show that the relative importance of key participants changed from the conspiracy to the post-murder phases, whereby strong, trusted ties gained prominence over the mostly business-oriented network of the conspiracy phase.
Research on the Dynamic Pricing and Service Decisions in the Reverse Supply Chain considering Consumers’ Service Sensitivity
Authors: Zhang Jixiang , Zhu Chen
Published: November 2020

In this work, considering consumers’ service sensitivity for the third-party collector in the reverse supply chain, a game model (including a manufacturer and a third-party collector) with dual-channel recycling structure was established. Based on the bounded rationality, we used dynamics theory to analyze the dynamic service and pricing decisions of the manufacturer and the third-party collector in the process of collecting waste products. The results showed that adjustment speeds of pricing and service decisions have great effect on the stability of recycling market, and the recycling market is most sensitive to the change of third-party collectors’ service decision. When the recycling market is in chaos, it has a strong initial value sensitivity. In addition, we found that increase of consumers’ service sensitivity and service cost parameter had negative effect on stability of the recycling market; there is a reverse relationship between price adjustment parameters and the service adjustment parameter. With the increase of service adjustment parameter, the bifurcation point of the system will appear more quickly. Finally, chaotic market can be controlled by the feedback control method.
Circularity Evaluation of Alternative Concepts During Early Product Design and Development
Authors: Kamp Albæk Julie , Shahbazi Sasha , McAloone Tim C. , Pigosso Daniela C. A.
Published: November 2020

Product design and development are essential for a circular transition. Circularity decisions, such as those concerning the type of material, assembly method, and expected lifespan, made during the early design stages will significantly influence a product’s quality, cost, esthetics, sustainability, and circularity performance over the product lifecycle. However, circularity is not often considered in the early stages of product design and development. This paper presents the development of the concept circularity evaluation tool (CCET), which aims to support the evaluation of alternative product concepts in terms of their circularity potential in the early stages of product design and development. The CCET was iteratively developed based on an extensive literature review of the success criteria for tool development, guidelines, and existing tools for circular product design and development and strong collaboration with manufacturing companies. The tool was tested and verified at four manufacturing companies in Nordic countries. The tool has been proven useful for evaluating the circularity of products and supportive in the decision-making process in the early stages of product design and development.