bluuebunny/crossref_metadata_2025 huggingface dataset:
Oncologic outcome with versus without target volume compartmentalization in postoperative radiotherapy for oral cavity squamous cell carcinoma
Authors: Riggenbach Elena , Waser Manuel , Mueller Simon A. , Aebersold Daniel M. , Giger Roland , Elicin Olgun
Published: March 2024

Background and purpose

The volume treated with postoperative radiation therapy (PORT) in patients with oral cavity squamous cell carcinoma (OCSCC) is a mediator of toxicity affecting quality of life. Current guidelines only allow for very limited reduction of PORT volumes. This study investigated the safety and efficacy of de-intensified PORT for patients with OCSCC by refined compartmentalization of the treatment volume.

Materials and methods

This retrospective cohort study identified 103 OCSCC patients treated surgically from 2014 to 2019 with a loco-regional risk profile qualifying for PORT according to guidelines. PORT was administered only to the at-risk compartment and according to a refined compartmentalization concept (CC). Oncological outcome of this CC cohort was compared to a historical cohort (HC) of 98 patients treated before the CC was implemented.

Results

Median follow-up time was 4.5 and 4.8 years in the CC and HC cohorts, respectively. In the CC cohort, a total of 72 of 103 patients (70%) had a pathological risk profile that allowed for further compartmentalization and, hence, received a reduced treatment volume or omission of PORT altogether. Loco-regional control at 3 and 5 years was 77% and 73% in the CC cohort versus 78% and 73% in the HC (p = 0.93), progression-free survival was 72% and 64% versus75% and 68% (p = 0.58), respectively. Similarly, no statistically significant difference was seen in other outcome measures.

Conclusions

De-intensified PORT limiting the treatment volume to the at-risk compartment or avoiding PORT altogether for low-risk patients with OCSCC does not seem to compromise disease control in this retrospective comparison. Based on these hypothesis-generating findings, a prospective study is being planned.
Research progress on the antitumor effects of harmine
Authors: Hu Yonghua , Yu Xiaoli , Yang Lei , Xue Gaimei , Wei Qinglin , Han Zhijian , Chen Hao
Published: March 2024

Harmine is a naturally occurring β-carboline alkaloid originally isolated from Peganum harmala. As a major active component, harmine exhibits a broad spectrum of pharmacological properties, particularly remarkable antitumor effects. Recent mechanistic studies have shown that harmine can inhibit cancer cell proliferation and metastasis through epithelial-to-mesenchymal transition, cell cycle regulation, angiogenesis, and the induction of tumor cell apoptosis. Furthermore, harmine reduces drug resistance when used in combination with chemotherapeutic drugs. Despite its remarkable antitumor activity, the application of harmine is limited by its poor solubility and toxic side effects, particularly neurotoxicity. Novel harmine derivatives have demonstrated strong clinical application prospects, but further validation based on drug activity, acute toxicity, and other aspects is necessary. Here, we present a review of recent research on the action mechanism of harmine in cancer treatment and the development of its derivatives, providing new insights into its potential clinical applications and strategies for mitigating its toxicity while enhancing its efficacy.
Kinetic modeling of the plasma pharmacokinetic profiles of ADAMTS13 fragment and its Fc-fusion counterpart in mice
Authors: Kwak Heechun , Kim Min-Soo , Kim Suyong , Kim Jiyoung , Aoki Yasunori , Chung Suk-Jae , Nam Hyun-Ja , Lee Wooin
Published: March 2024

Introduction: Fusion of the fragment crystallizable (Fc) to protein therapeutics is commonly used to extend the circulation time by enhancing neonatal Fc-receptor (FcRn)-mediated endosomal recycling and slowing renal clearance. This study applied kinetic modeling to gain insights into the cellular processing contributing to the observed pharmacokinetic (PK) differences between the novel recombinant ADAMTS13 fragment (MDTCS) and its Fc-fusion protein (MDTCS-Fc).

Methods: For MDTCS and MDTCS-Fc, their plasma PK profiles were obtained at two dose levels following intravenous administration of the respective proteins to mice. The plasma PK profiles of MDTCS were fitted to a kinetic model with three unknown protein-dependent parameters representing the fraction recycled (FR) and the rate constants for endocytosis (kup, for the uptake into the endosomes) and for the transfer from the plasma to the interstitial fluid (kpi). For MDTCS-Fc, the model was modified to include an additional parameter for binding to FcRn. Parameter optimization was done using the Cluster Gauss-Newton Method (CGNM), an algorithm that identifies multiple sets of approximate solutions (“accepted” parameter sets) to nonlinear least-squares problems.

Results: As expected, the kinetic modeling results yielded the FR of MDTCS-Fc to be 2.8-fold greater than that of MDTCS (0.8497 and 0.3061, respectively). In addition, MDTCS-Fc was predicted to undergo endocytosis (the uptake into the endosomes) at a slower rate than MDTCS. Sensitivity analyses identified the association rate constant (kon) between MDTCS-Fc and FcRn as a potentially important factor influencing the plasma half-life in vivo.

Discussion: Our analyses suggested that Fc fusion to MDTCS leads to changes in not only the FR but also the uptake into the endosomes, impacting the systemic plasma PK profiles. These findings may be used to develop recombinant protein therapeutics with extended circulation time.
Viscoelastic material properties determine the contact mechanics of hydrogel spheres
Authors: Shakya Chandan , van der Gucht Jasper , Dijksman Joshua A.
Published: March 2024

Granular materials are ubiquitous in nature and industry; their mechanical behavior has been a subject of academic and engineering interest for centuries. One of the reasons for their rather complex mechanical behavior is that stresses exerted on a granular material propagate only through contacts between the grains. These contacts can change as the packing evolves. This makes any deformation and mechanical response from a granular packing a function of the nature of contacts between the grains and the material response of the material the grains are made of. We present a study in which we isolate the role of the grain material in the contact forces acting between two particles sliding past each other. By using hydrogel particles, we find that a viscoelastic material model, in which the shear modulus decays with time, coupled with a simple Coulomb friction model, captures the experimental results. The results suggest that particle material evolution itself may play a role in the collective behavior of granular materials.
Anatomical, chemical and endophytic fungal diversity of a Qi-Nan clone of Aquilaria sinensis (Lour.) Spreng with different induction times
Authors: Li Xiaofei , Fang Xiaoying , Cui Zhiyi , Hong Zhou , Liu Xiaojin , Li Gaiyun , Hu Houzhen , Xu Daping
Published: March 2024

Recently, some new Qi-Nan clones of Aquilaria sinensis (Lour.) Spreng which intensively produces high-quality agarwood have been identified and propagated through grafting techniques. Previous studies have primarily focused on ordinary A. sinensis and the differences in composition when compared to Qi-Nan and ordinary A. sinensis. There are few studies on the formation mechanism of Qi-Nan agarwood and the dynamic changes in components and endophytic fungi during the induction process. In this paper, the characteristics, chemical composition, and changes in endophytic fungi of Qi-Nan agarwood induced after 1 year, 2 years, and 3 years were studied, and Qi-Nan white wood was used as the control. The results showed that the yield of Qi-Nan agarwood continued to increase with the induction time over a period of 3 years, while the content of alcohol extract from Qi-Nan agarwood reached its peak at two years. During the formation of agarwood, starch and soluble sugars in xylem rays and interxylary phloem are consumed and reduced. Most of the oily substances in agarwood were filled in xylem ray cells and interxylary phloem, and a small amount was filled in xylem vessels. The main components of Qi-Nan agarwood are also chromones and sesquiterpenes. With an increasing induction time, the content of sesquiterpenes increased, while the content of chromones decreased. The most abundant chromones in Qi-Nan agarwood were 2-(2-Phenethyl) chromone, 2-[2-(3-Methoxy-4-hydroxyphenyl) ethyl] chromone, and2-[2-(4-Methoxyphenyl) ethyl] chromone. Significant differences were observed in the species of the endophytic fungi found in Qi-Nan agarwood at different induction times. A total of 4 phyla, 73 orders, and 448 genera were found in Qi-Nan agarwood dominated by Ascomycota and Basidiomycota. Different induction times had a significant effect on the diversity of the endophytic fungal community in Qi-Nan. After the induction of agarwood formation, the diversity of Qi-Nan endophytic fungi decreased. Correlation analysis showed that there was a significant positive correlation between endophytic fungi and the yield, alcohol extract content, sesquiterpene content, and chromone content of Qi-Nan agarwood, which indicated that endophytic fungi play a role in promoting the formation of Qi-Nan agarwood. Qi-Nan agarwood produced at different induction times exhibited strong antioxidant capacity. DPPH free radical scavenging activity and reactive oxygen species clearance activity were significantly positively correlated with the content of sesquiterpenes and chromones in Qi-Nan agarwood.
Exposure to benzene, toluene, ethylbenzene, and xylene (BTEX) at Nigeria's petrol stations: a review of current status, challenges and future directions
Authors: Anigilaje Emmanuel Ademola , Nasir Zaheer Ahmad , Walton Christopher
Published: March 2024

Introduction

In Nigeria, because of increasing population, urbanization, industrialization, and auto-mobilization, petrol is the most everyday non-edible commodity, and it is the leading petroleum product traded at the proliferating Nigeria's petrol stations (NPSs). However, because of inadequate occupational health and safety (OHS) regulatory measures, working at NPSs exposes petrol station workers (PSWs) to a large amount of hazardous benzene, toluene, ethylbenzene, and xylene (BTEX) compounds.

Methods

Studies on BTEX exposures among Nigerian PSWs are scarce. Thus, constraints in quantifying the health risks of BTEX limit stakeholders' ability to design practical risk assessment and risk control strategies. This paper reviews studies on the OHS of Nigerian PSWs at the NPSs.

Results

Although knowledge, attitude, and practices on OHS in NPSs vary from one Nigeria's study setting to another, generally, safety practices, awareness about hazards and personal protective equipment (PPE), and the use of PPE among PSWs fell below expectations. Additionally, air quality at NPSs was poor, with a high content of BTEX and levels of carbon monoxide, hydrogen sulfide, particulate matter, and formaldehyde higher than the World Health Organization guideline limits.

Discussion

Currently, regulatory bodies' effectiveness and accountability in safeguarding OHS at NPSs leave much to be desired. Understanding the OHS of NPSs would inform future initiatives, policies, and regulations that would promote the health and safety of workers at NPSs. However, further studies need to be conducted to describe the vulnerability of PSWs and other Nigerians who are occupationally exposed to BTEX pollution. More importantly, controlling air pollution from hazardous air pollutants like BTEX is an essential component of OHS and integral to attaining the Sustainable Development Goals (SDG) 3, 7, and 11.
Profiles in neglect of older adult care workers in a long-term care facility: a latent profile analysis
Authors: Yan Chang , Wang Caili , Ding Xinshu , Sun Lefei , Gao Wei , Liu Deshan
Published: March 2024

Background

Neglect is a common form of abuse, and long-term care facilities record higher incidences of this abuse. Given that older adult care workers are the main workforce in these facilities, their neglectful behavior requires public health attention. Internal individual characteristics can lead to older adult abuse, and managing workers who abuse older adults may require various methods. This study aimed to identify the profiles of neglect among older adult care workers in long-term care facilities and explore the influencing factors of neglect.

Methods

In this cross-sectional study, a convenience sample of older adult care workers from 15 long-term care facilities in Shandong Province (N = 421) completed a questionnaire on the characteristics associated with neglect. Latent profile analysis was used to identify distinct neglect profiles and promote the understanding of individual characteristics associated with varying levels of neglect. One-way analysis of variance and multivariate logistic regression analyses were used to examine the population characteristic differences.

Results

Older adult care workers exhibited three neglect profiles, namely, the “low-risk group,” “medium-risk group,” and “high-risk group.” Males, participants with no employment qualification certificate, and those who did not attend regular training represented the majority of those in the “high-risk group.” Participants with a monthly income of more than ¥ 4,000 and nursing 1–2 older adults simultaneously represented the majority of those in the “low-risk group.”

Conclusion

Long-term care facility administrators should tailor interventions to individual care worker profiles to reduce neglect behaviors and improve care levels.
Challenging the status quo: a framework for mechanistic and human-relevant cardiovascular safety screening
Authors: Berridge Brian , Pierson Jennifer , Pettit Syril , Stockbridge Norman
Published: March 2024

Traditional approaches to preclinical drug safety assessment have generally protected human patients from unintended adverse effects. However, these assessments typically occur too late to make changes in the formulation or in phase 1 and beyond, are highly dependent on animal studies and have the potential to lead to the termination of useful drugs due to liabilities in animals that are not applicable in patients. Collectively, these elements come at great detriment to both patients and the drug development sector. This phenomenon is particularly problematic in the area of cardiovascular safety assessment where preclinical attrition is high. We believe that a more efficient and translational approach can be defined. A multi-tiered assessment that leverages our understanding of human cardiovascular biology, applies human cell-based in vitro characterizations of cardiovascular responses to insult, and incorporates computational models of pharmacokinetic relationships would enable earlier and more translational identification of human-relevant liabilities. While this will take time to develop, the ultimate goal would be to implement such assays both in the lead selection phase as well as through regulatory phases.
Effects of different dietary threonine and glycine supplies in broilers fed low-protein diets
Authors: Strifler Patrik , Horváth Boglárka , Such Nikoletta , Dublecz Károly , Pál László
Published: March 2024

The reduction of crude protein (CP) content of broiler diets with balanced amino acid supply can increase the nitrogen (N) utilization efficiency and reduce ammonia emission, the risk of many health problems in birds. Feeding low protein (LP) diets without the impairment of performance traits needs the optimized dietary levels of threonine (Thr) and the non-essential amino acid (AA) glycine (Gly) and serine (Ser). However, the required concentrations and interactions of Thr and Gly + Ser, expressed as Gly equivalent (Glyequi), in LP diets are not fully understood. Therefore, the aim of this study was to investigate the effects of three LP (LP1–3) grower (11–24 days) and finisher (25–35 days) diets with 2% CP reduction compared to the control (C), differing in standardized ileal digestible (SID) Thr to lysine (Lys) ratio (C, LP1, LP3: 63%, LP2: 72%) and Glyequi levels (C: 15.65 g/kg, LP1: 13.74 g/kg, LP2: 13.70 g/kg, LP3: 15.77). The LP treatments did not impair the performance traits of broilers. The LP2 treatment with increased SID Thr-to-Lys ratio (+9.0%) resulted in significantly higher body weight gain and a more advantageous feed conversion ratio in the whole fattening compared to the control treatment with normal CP level (p < 0.05). The LP3 treatment containing swine meat meal with similar Glyequi levels compared to the normal CP treatment led to the most advantageous feed conversion ratio in the finisher phase and the highest nitrogen retention efficiency (p < 0.05). However, the LP3 treatment with a high starch-to-CP ratio negatively influenced the relative carcass weight and the ratio of abdominal fat of broilers (p < 0.05).
Complete Mitogenome sequencing of the fish louse Argulus japonicus (Crustacea: Branchiura): Comparative analyses and phylogenetic implications
Authors: Wang Lidan , Hu Zun , Wang Zhao , Zhu Pengchen , Wei Guoshan , Fan Xinyi , Huang Jiali , Wang Ruixi , Wang Hui , Xie Yue
Published: March 2024

The fish louse Argulus japonicus, a branchiuran crustacean of the Argulidae family, is attracting increasing attention because of its parasitic tendencies and significant health threats to global fish farming. The mitogenomes can yield a foundation for studying epidemiology, genetic diversity, and molecular ecology and therefore may be used to assist in the surveillance and control of A. japonicus. In this study, we sequenced and assembled the complete mitogenome of A. japonicus to shed light on its genetic and evolutionary blueprint. Our investigation indicated that the 15,045-bp circular genome of A. japonicus encodes 13 protein-coding genes (PCGs), 22 transfer RNAs (tRNAs), and 2 ribosomal RNAs (rRNAs) with significant AT and GC skews. Comparative genomics provided an evolutionary scenario for the genetic diversity of 13 PCGs: all were under purifying selection, with cox1 and nad6 having the lowest and highest evolutionary rates, respectively. Genome-wide phylogenetic trees established a close relationship between species of the families Argulidae (Arguloida) and Armilliferidae (Porocephalida) within Crustacea, and further, A. japonicus and Argulus americanus were determined to be more closely related to each other than to others within the family Argulidae. Single PCG-based phylogenies supported nad1 and nad6 as the best genetic markers for evolutionary and phylogenetic studies for branchiuran crustaceans due to their similar phylogenetic topologies with those of genome-based phylogenetic analyses. To sum up, these comprehensive mitogenomic data of A. japonicus and related species refine valuable marker resources and should contribute to molecular diagnostic methods, epidemiological investigations, and ecological studies of the fish ectoparasites in Crustacea.
Phthalate Exposure and Neurotoxicity in Children: A Systematic Review and Meta-analysis
Authors: Antoniou Evangelia E. , Otter Rainer
Published: March 2024

Objectives: This systematic review aims to assess the relationship between prenatal and childhood exposure to phthalates and neurodevelopmental outcomes, identifying periods of heightened susceptibility. Data sources considered studies examining repeated phthalate exposure during pregnancy and childhood on neurodevelopment.

Methods: Evaluation included bias risk and study quality criteria. Evidence was synthesized by groups of low and high phthalate molecular weight and exposure measured prenatally and postnatally and outcome measured in childhood. Beta coefficients and their standard errors were extracted, leading to meta-analyses of various neurodevelopmental outcomes: cognition, motor skills, language, behavior, and temperament.

Results: Eleven pregnancy and birth cohort studies were identified as relevant. For each phthalate group and outcome combination, there was low or very low evidence of an association, except for prenatal and postnatal phthalate exposure and behavioral development and postnatal exposure and cognition.

Conclusion: The estimated effects sizes were relatively small and strong evidence for periods of heightened susceptibility could not be elucidated. No distinction between phthalates of low molecular weight and those of high molecular weight with regards to the outcomes was found.
Numerical Investigation and Optimization of a Morphing Airfoil Designed for Lower Reynolds Number
Authors: Lukić Nebojša , Ivanov Toni , Svorcan Jelena , Simonović Aleksandar
Published: March 2024

A novel concept of morphing airfoils, capable of changing camber and thickness, is proposed. A variable airfoil shape, defined by six input parameters, is achieved by allowing the three spinal points (at fixed axial positions) to slide vertically, while the upper and lower surfaces are determined by the lengths of the three corresponding ribs that are perpendicular to the spine. Thus, it is possible to find the most appropriate geometric configuration for a wide range of possible operating conditions often present with contemporary unmanned aerial vehicles. Shape optimizations for different Reynolds numbers and different cost functions are performed by coupling a genetic algorithm with simple panel method flow calculations. The obtained airfoils are presented and compared, whereas the proposed concept is validated by more advanced flow simulations. It appears that improvements in aerodynamic performance of nearly 20% can be expected at Re ranging from 0.05 × 106 to 0.1 × 106. The proposed methodology shows promise and can be applied to different types of lifting surfaces, including wing, tail or propeller blade segments. To check the viability of this method for producing airfoils that can be used in a practical sense, structural analysis of one of the obtained geometries using a simplified 1D finite element method as well as a more detailed 3D analysis are performed. The model is then 3D-printed on a fused deposition modeling (FDM) printer with a polyethylene terephthalate glycol (PETG) filament, and the capability of the airfoil to adequately morph between the two desired geometries is experimentally shown.
Lunar Rover Collaborated Path Planning with Artificial Potential Field-Based Heuristic on Deep Reinforcement Learning
Authors: Lu Siyao , Xu Rui , Li Zhaoyu , Wang Bang , Zhao Zhijun
Published: March 2024

The International Lunar Research Station, to be established around 2030, will equip lunar rovers with robotic arms as constructors. Construction requires lunar soil and lunar rovers, for which rovers must go toward different waypoints without encountering obstacles in a limited time due to the short day, especially near the south pole. Traditional planning methods, such as uploading instructions from the ground, can hardly handle many rovers moving on the moon simultaneously with high efficiency. Therefore, we propose a new collaborative path-planning method based on deep reinforcement learning, where the heuristics are demonstrated by both the target and the obstacles in the artificial potential field. Environments have been randomly generated where small and large obstacles and different waypoints are created to collect resources, train the deep reinforcement learning agent to propose actions, and lead the rovers to move without obstacles, finish rovers’ tasks, and reach different targets. The artificial potential field created by obstacles and other rovers in every step affects the action choice of the rover. Information from the artificial potential field would be transformed into rewards in deep reinforcement learning that helps keep distance and safety. Experiments demonstrate that our method can guide rovers moving more safely without turning into nearby large obstacles or collision with other rovers as well as consuming less energy compared with the multi-agent A-Star path-planning algorithm with improved obstacle avoidance method.
Identification of Key Risk Hotspots in Mega-Airport Surface Based on Monte Carlo Simulation
Authors: Tian Wen , Zhou Xuefang , Yin Jianan , Li Yuchen , Zhang Yining
Published: March 2024

The complex layout of the airport surface, coupled with interrelated vehicle behaviors and densely mixed traffic flows, frequently leads to operational conflict risks. To address this issue, research was conducted on the recognition of characteristics and risk assessment for airport surface operations in mixed traffic flows. Firstly, a surface topological network model was established based on the analysis of the physical structure features of the airport surface. Based on the Monte Carlo simulation method, the simulation framework for airport surface traffic operations was proposed, enabling the simulation of mixed traffic flows involving aircraft and vehicles. Secondly, from various perspectives, including topological structural characteristics, network vulnerabilities, and traffic complexity, a comprehensive system for feature indices and their measurement methods was developed to identify risk hotspots in mixed traffic flows on the airport surface, which facilitated the extraction of comprehensive risk elements for any node’s operation. Finally, a weighting rule for risk hotspot feature indices based on the CRITIC–entropy method was designed, and a risk assessment method for surface operations based on TOPSIS–gray relational analysis was proposed. This method accurately measured risk indices for airport surface operations hotspots. Simulations conducted at Shenzhen Bao’an International Airport demonstrate that the proposed methods achieve high simulation accuracy. The identified surface risk hotspots closely matched actual conflict areas, resulting in a 20% improvement in the accuracy of direct risk hotspot identification compared to simulation experiments. Additionally, 10.9% of nodes in the airport surface network were identified as risk hotspots, including 3 nodes with potential conflicts between aircraft and ground vehicles and 21 nodes with potential conflicts between aircraft. The proposed methods can effectively provide guidance for identifying potential “aircraft–vehicle” conflicts in complex airport surface layouts and scientifically support informed decisions in airport surface operation safety management.
An Eight-Node Non-Conforming Generalized Partial Hybrid Element and Its Application in Stress Analysis of Repaired Composite Laminate Structures
Authors: Xing Ruishan , Li Gang , Wang Fan , Yang Yang
Published: March 2024

To ensure the overall continuity of displacement and out-of-plane stress in composite laminate structures and to quantitatively analyze the mechanical properties of composite materials after damage or repair, a finite element solution method is applied based on the modified generalized H–R variational principle. This method utilizes an eight-node non-conforming generalized partial hybrid element (NCGPME8). The partial hybrid model established with this hybrid element can accurately satisfy the out-of-plane stress boundary conditions of the structure, ensuring the continuity of out-of-plane stress. Numerical examples are used to validate that this hybrid model can effectively compute thick and thin laminate structures with high accuracy and rapid convergence of out-of-plane stress. Finally, considering the insensitivity to irregular meshes and the accuracy in calculating in-plane stress, this method is propagated by element coefficient deduction or element material replacement, then employed to analyze the in-plane and out-of-plane stress distributions of laminates with damage from stepwise grinding perforations, and laminates repaired in a stepwise fashion. Stress and displacement at different locations on the laminates are compared and analyzed, leading to a quantitative assessment of the impact of damage and repair on the stress distribution of the laminates.
Restraining Staphylococcus aureus Virulence Factors and Quorum Sensing through Lactic Acid Bacteria Supernatant Extracts
Authors: Díaz Myriam Anabel , Vega-Hissi Esteban Gabriel , Blázquez María Amparo , Alberto María Rosa , Arena Mario Eduardo
Published: March 2024

The escalating prevalence of antibiotic-resistant bacteria poses a grave threat to human health, necessitating the exploration of novel alternatives to conventional antibiotics. This study investigated the impact of extracts derived from the supernatant of four lactic acid bacteria strains on factors contributing to the pathogenicity of three Staphylococcus aureus strains. The study evaluated the influence of lactic acid bacteria supernatant extracts on the growth, biofilm biomass formation, biofilm metabolic activity, and biofilm integrity of the S. aureus strains. Additionally, the impact on virulence factors (hemolysin and coagulase) was examined. Gas chromatography coupled with mass spectrometry was used to identify the bioactive compounds in the extracts, while molecular docking analyses explored potential interactions. Predominantly, the extracts contain eight 2,5-diketopiperazines, which are cyclic forms of peptides. The extracts demonstrated inhibitory effects on biofilm formation, the ability to disrupt mature biofilms, and reduce the biofilm cell metabolic activity of the S. aureus strains. Furthermore, they exhibited the ability to inhibit α-hemolysin production and reduce coagulase activity. An in silico docking analysis reveals promising interactions between 2,5-diketopiperazines and key proteins (SarA and AgrA) in S. aureus, confirming their antivirulence and antibiofilm activities. These findings suggest that 2,5-diketopiperazines could serve as a promising lead compound in the fight against antibiotic-resistant S. aureus.
Updates to Sections in the Applied Sciences Journal
Authors: Kobayashi Takayoshi
Published: March 2024

The journal Applied Sciences was established in 2011, covering five broad subjects and thirty-two narrow sections [...]
Safety Evaluation of Existing R.C. Buildings: Uncertainties Due to the Location of In Situ Tests
Authors: Sepe Vincenzo , Diaferio Mariella , Caraccio Roberta
Published: March 2024

The paper aimed to investigate the influence, on the assessment of the structural safety level of an existing r.c building, of the different choices that the technician in charge of a structural evaluation (the “analyst”) can make regarding the structural elements to be tested to obtain a prescribed level of knowledge. To this end, the case study of a reinforced concrete framed structure built in the 1960s in Italy was investigated by means of numerical analyses. The probability distribution of the estimated safety levels was evaluated in the paper by means of a Monte Carlo approach, considering the alternative selections of elements done by a large number of analysts, and the probability of unsuccessful safety estimations is discussed for the knowledge levels considered in the Italian technical codes and the Eurocodes.
A Training Program Using Modified Joystick-Operated Ride-on Toys to Complement Conventional Upper Extremity Rehabilitation in Children with Cerebral Palsy: Results from a Pilot Study
Authors: Srinivasan Sudha , Kumavor Patrick , Morgan Kristin
Published: March 2024

The pilot study assessed the utility of a training program using modified, commercially available dual-joystick-operated ride-on toys to promote unimanual and bimanual upper extremity (UE) function in children with cerebral palsy (CP). The ride-on-toy training was integrated within a 3-week, intensive, task-oriented training camp for children with CP. Eleven children with hemiplegia between 4 and 10 years received the ride-on-toy training program 20–30 min/day, 5 days/week for 3 weeks. Unimanual motor function was assessed using the Quality of Upper Extremity Skills Test (QUEST) before and after the camp. During ride-on-toy training sessions, children wore activity monitors on both wrists to assess the duration and intensity of bimanual UE activity. Video data from early and late sessions were coded for bimanual UE use, independent navigation, and movement bouts. Children improved their total and subscale QUEST scores from pretest to post-test while increasing moderate activity in their affected UE from early to late sessions, demonstrating more equal use of both UEs across sessions. There were no significant changes in the rates of movement bouts from early to late sessions. We can conclude that joystick-operated ride-on toys function as child-friendly, intrinsically rewarding tools that can complement conventional therapy and promote bimanual motor functions in children with CP.
Design, Manufacture, and Characterization of a Critical-Sized Gradient Porosity Dual-Material Tibial Defect Scaffold
Authors: Lee Ming-Chan , Pan Cheng-Tang , Chen Wen-Fan , Lin Meng-Chi , Shiue Yow-Ling
Published: March 2024

This study proposed a composite tibia defect scaffold with radial gradient porosity, utilizing finite element analysis to assess stress in the tibial region with significant critical-sized defects. Simulations for scaffolds with different porosities were conducted, designing an optimal tibia defect scaffold with radial gradient porosity for repairing and replacing critical bone defects. Radial gradient porosity scaffolds resulted in a more uniform stress distribution, reducing titanium alloy stiffness and alleviating stress shielding effects. The scaffold was manufactured using selective laser melting (SLM) technology with stress relief annealing to simplify porous structure fabrication. The study used New Zealand white rabbits’ tibia defect sites as simulation parameters, reconstructing the 3D model and implanting the composite scaffold. Finite element analysis in ANSYS-Workbench simulated forces under high-activity conditions, analyzing stress distribution and strain. In the simulation, the titanium alloy scaffold bore a maximum stress of 122.8626 MPa, while the centrally encapsulated HAp material delivered 27.92 MPa. The design demonstrated superior structural strength, thereby reducing stress concentration. The scaffold was manufactured using SLM, and the uniform design method was used to determine a collection of optimum annealing parameters. Nanoindentation and compression tests were used to determine the influence of annealing on the elastic modulus, hardness, and strain energy of the scaffold.
NMDA Receptor Antagonist Memantine Ameliorates Experimental Autoimmune Encephalomyelitis in Aged Rats
Authors: Bufan Biljana , Ćuruvija Ivana , Blagojević Veljko , Grujić-Milanović Jelica , Prijić Ivana , Radosavljević Tatjana , Samardžić Janko , Radosavljevic Milica , Janković Radmila , Djuretić Jasmina
Published: March 2024

Aging is closely related to the main aspects of multiple sclerosis (MS). The average age of the MS population is increasing and the number of elderly MS patients is expected to increase. In addition to neurons, N-methyl-D-aspartate receptors (NMDARs) are also expressed on non-neuronal cells, such as immune cells. The aim of this study was to investigate the role of NMDARs in experimental autoimmune encephalomyelitis (EAE) in young and aged rats. Memantine, a non-competitive NMDAR antagonist, was administered to young and aged Dark Agouti rats from day 7 after immunization. Antagonizing NMDARs had a more favourable effect on clinical disease, reactivation, and apoptosis of CD4+ T cells in the target organ of aged EAE rats. The expression of the fractalkine receptor CX3CR1 was increased in memantine-treated rats, but to a greater extent in aged rats. Additionally, memantine increased Nrf2 and Nrf2-regulated enzymes’ mRNA expression in brain tissue. The concentrations of superoxide anion radicals, malondialdehyde, and advanced oxidation protein products in brain tissue were consistent with previous results. Overall, our results suggest that NMDARs play a more important role in the pathogenesis of EAE in aged than in young rats.
Neuromodulation’s Role in Functional Restoration in Paraplegic and Quadriplegic Patients
Authors: Abd-Elsayed Alaa , Robinson Christopher L. , Shehata Peter , Koh Yerin , Patel Milan , Fiala Kenneth J.
Published: March 2024

Neuromodulation is an alternative, minimally invasive treatment option that, at times, is used as a last resort for chronic pain conditions that are often refractory to other treatment modalities. Moreover, it offers promising prospects for individuals grappling with the formidable challenges posed by paraplegia and quadriplegia resulting from spinal cord injuries. This review article provides a comprehensive assessment of current treatment modalities specifically tailored for paraplegic and quadriplegic patients. We aim to evaluate the existing surgical and non-surgical interventions while delving into the role of neuromodulation in the restoration of function for individuals afflicted with these debilitating conditions. Additionally, we review the efficacy, limitations, and comparative outcomes of diverse treatment strategies available for the management of paraplegia and quadriplegia. Emphasizing the critical need for effective interventions beyond the initial 24 h surgical window, we elucidate the challenges associated with conventional therapies and their limited success in achieving comprehensive functional restoration. Central to this review is an in-depth exploration of neuromodulation’s transformative potential in ameliorating the deficits caused by spinal cord injuries. With a particular focus on spinal cord stimulation (SCS), we analyze and compare the outcomes of neuromodulation modalities and traditional treatment regimens, shedding light on the promising strides made in fostering sensory perception, motor function, and patient satisfaction.
miRNAs: From Master Regulators of Gene Expression to Biomarkers Involved in Intercellular Communication
Authors: Levantini Elena , Rizzo Milena
Published: March 2024

MicroRNAs (miRNAs) are non-coding RNAs that act as master regulators of gene expression, fine-tuning the activity of thousands of genes in our cells, by modulating gene expression at the post-transcriptional level [...]
Single-Center Experience of Pediatric Cystic Kidney Disease and Literature Review
Authors: Grlić Sara , Gregurović Viktorija , Martinić Mislav , Davidović Maša , Kos Ivanka , Galić Slobodan , Fištrek Prlić Margareta , Vuković Brinar Ivana , Vrljičak Kristina , Lamot Lovro
Published: March 2024

Introduction: Pediatric cystic kidney disease (CyKD) includes conditions characterized by renal cysts. Despite extensive research in this field, there are no reliable genetics or other biomarkers to estimate the phenotypic consequences. Therefore, CyKD in children heavily relies on clinical and diagnostic testing to predict the long-term outcomes. Aim: A retrospective study aimed to provide a concise overview of this condition and analyze real-life data from a single-center pediatric CyKD cohort followed during a 12-year period. Methods and Materials: Medical records were reviewed for extensive clinical, laboratory, and radiological data, treatment approaches, and long-term outcomes. Results: During the study period, 112 patients received a diagnosis of pediatric CyKD. Male patients were more involved than female (1:0.93). Fifty-six patients had a multicystic dysplastic kidney; twenty-one of them had an autosomal dominant disorder; fifteen had an isolated renal cyst; ten had been diagnosed with autosomal recessive polycystic kidney disease; three had the tuberous sclerosis complex; two patients each had Bardet–Biedl, Joubert syndrome, and nephronophthisis; and one had been diagnosed with the trisomy 13 condition. Genetic testing was performed in 17.9% of the patients, revealing disease-causing mutations in three-quarters (75.0%) of the tested patients. The most commonly presenting symptoms were abdominal distension (21.4%), abdominal pain (15.2%), and oligohydramnios (12.5%). Recurrent urinary tract infections (UTI) were documented in one-quarter of the patients, while 20.5% of them developed hypertension during the long-term follow-up. Antibiotic prophylaxis and antihypertensive treatment were the most employed therapeutic modalities. Seventeen patients progressed to chronic kidney disease (CKD), with thirteen of them eventually reaching end-stage renal disease (ESRD). The time from the initial detection of cysts on an ultrasound (US) to the onset of CKD across the entire cohort was 59.0 (7.0–31124.0) months, whereas the duration from the detection of cysts on an US to the onset of ESRD across the whole cohort was 127.0 (33.0–141.0) months. The median follow-up duration in the cohort was 3.0 (1.0–7.0) years. The patients who progressed to ESRD had clinical symptoms at the time of initial clinical presentation. Conclusion: This study is the first large cohort of patients reported from Croatia. The most common CyKD was the multicystic dysplastic kidney disease. The most common clinical presentation was abdominal distention, abdominal pain, and oliguria. The most common long-term complications were recurrent UTIs, hypertension, CKD, and ESRD.
Predicting Postoperative Lung Function in Patients with Lung Cancer Using Imaging Biomarkers
Authors: Kwon Oh-Beom , Lee Hae-Ung , Park Ha-Eun , Choi Joon-Young , Kim Jin-Woo , Lee Sang-Haak , Yeo Chang-Dong
Published: March 2024

There have been previous studies conducted to predict postoperative lung function with pulmonary function tests (PFTs). Computing tomography (CT) can quantitatively measure small airway walls’ thickness, lung volume, pulmonary vessel volume, and emphysema area, which reflect the severity of respiratory diseases. These measurements are considered imaging biomarkers. This study aimed to predict postoperative lung function with imaging biomarkers. A retrospective analysis of 79 patients with lung cancer who had undergone lung surgery was completed. Postoperative lung function measured by forced expiratory volume in one second (FEV1) was defined as an outcome. Preoperative clinico-pathological parameters and imaging biomarkers representing airway walls’ thickness, severity of emphysema, total lung volume, and pulmonary vessel volume were measured quantitatively in chest CT by an automated segmentation software, AVIEW COPD. Pi1 was defined as the first percentile along the histogram of lung attenuation that represents the degree of emphysema. Wafw was defined as the airway thickness, which was calculated by the full-width at half-maximum method. Logistic and linear regressions were used to assess these variables. If the actual postoperative FEV1 was higher than the postoperative FEV1 projected by a formula, the group was considered to be preserved. Among the 79 patients, 16 of the patients were grouped as a non-preserved group, and 63 of them were grouped as a preserved group. The patients in the preserved FEV1 group had a higher vessel volume than the non-preserved group. Pi1 and Wafw were independent predictors of postoperative lung function. Imaging biomarkers can be considered significant variables in predicting postoperative lung function in patients with lung cancer.
Enhancing Signer-Independent Recognition of Isolated Sign Language through Advanced Deep Learning Techniques and Feature Fusion
Authors: Akdag Ali , Baykan Omer Kaan
Published: March 2024

Sign Language Recognition (SLR) systems are crucial bridges facilitating communication between deaf or hard-of-hearing individuals and the hearing world. Existing SLR technologies, while advancing, often grapple with challenges such as accurately capturing the dynamic and complex nature of sign language, which includes both manual and non-manual elements like facial expressions and body movements. These systems sometimes fall short in environments with different backgrounds or lighting conditions, hindering their practical applicability and robustness. This study introduces an innovative approach to isolated sign language word recognition using a novel deep learning model that combines the strengths of both residual three-dimensional (R3D) and temporally separated (R(2+1)D) convolutional blocks. The R3(2+1)D-SLR network model demonstrates a superior ability to capture the intricate spatial and temporal features crucial for accurate sign recognition. Our system combines data from the signer’s body, hands, and face, extracted using the R3(2+1)D-SLR model, and employs a Support Vector Machine (SVM) for classification. It demonstrates remarkable improvements in accuracy and robustness across various backgrounds by utilizing pose data over RGB data. With this pose-based approach, our proposed system achieved 94.52% and 98.53% test accuracy in signer-independent evaluations on the BosphorusSign22k-general and LSA64 datasets.
Correlating Quantitative and Genomic SARS-CoV-2 Wastewater Data with Clinical Metrics in Metropolitan Perth, Western Australia
Authors: Gazeley Jake , Lee Terence , Knight Daniel R. , Shivarev Alexander , Gordon Cameron , Speers David , Barth Dylan D. , Maticevic Jelena , Hodge Meredith , Armstrong Paul , Knight Paul , Sjollema Sandra , Levy Avram
Published: March 2024

Wastewater-based epidemiology (WBE) has emerged as a key method for the continuous monitoring of COVID-19 prevalence including circulating SARS-CoV-2 lineages. WBE addresses the limitations of traditional clinical COVID-19 surveillance such as clinical test availability, fluctuating testing rates, and increased reliance on rapid antigen tests. Our study in Perth, Western Australia found a significant positive correlation between SARS-CoV-2 concentrations in wastewater and clinical PCR positivity rates (rs = 0.772; p < 0.001) over an 18-month period that included four successive COVID-19 waves. A strong positive correlation was apparent between the proportions of SARS-CoV-2 lineages in wastewater and clinical cases within the same region (rs = 0.728, p < 0.001), including earlier detection of Omicron and recombinant lineages in wastewater before clinical case confirmation. The successful integration of WBE with healthcare data underscores its critical role in enhancing public health decision-making and pandemic management. This approach not only demonstrates the value of WBE in current global health surveillance efforts but also highlights the potential of WBE to address future public health challenges, as a comprehensive disease monitoring and response approach.
Biosurfactant-Assisted Phytoremediation of Diesel-Contaminated Soil by Three Different Legume Species
Authors: Meištininkas Rimas , Vaškevičienė Irena , Dikšaitytė Austra , Pedišius Nerijus , Žaltauskaitė Jūratė
Published: March 2024

This study aims to assess the impact of HydroBreak PLUS biosurfactant on the phytoremediation of diesel-contaminated soil by three legume plant species: Medicago sativa, Lotus corniculatus, and Melilotus albus. Legumes were grown in soil contaminated with diesel (4.0 g kg−1, 6.0 g kg−1) for 90 days, and the changes in soil diesel and nutrient concentrations, plant growth, and physiological parameters were measured. Diesel negatively affected the biomass production of all legumes, though the reduction in growth rate was observed only in L. corniculatus and M. albus. L. corniculatus had the highest diesel removal rate of 93%, M. albus had the lowest of 87.9%, and unplanted treatments had significantly lower diesel removal rates (up to 66.5%). The biosurfactant mitigated diesel-induced reduction in plant shoot and root weight and an increase in L. corniculatus root biomass (24.2%) were observed at 4.0 g kg−1 diesel treatment. The use of biosurfactant accelerated diesel removal from the soil, though the effect was diesel soil concentration and plant species-dependent. In unplanted treatments, the diesel removal rates increased by 16.4% and 6.9% in the treatments with 4 and 6 mg kg−1, respectively. The effect of biosurfactants on diesel removal by plants was less pronounced and reached 4.6% and 3.2% in the treatments with 4 and 6 mg kg−1, respectively. The study revealed that the phytoremediation efficiency could not be directly linked to plant physiological parameters as only M. sativa changes in plant growth corresponded well with photosystem II performance. Implementation of legumes and biosurfactants has a positive effect on soil quality by its enrichment with inorganic P and soluble phenols, while no enrichment in NO3− and NH4+ was observed.