bluuebunny/crossref_metadata_2025 huggingface dataset:
3-Alkoxy-1-Benzyl-5-Nitroindazole Derivatives Are Potent Antileishmanial Compounds
Authors: Mollineda-Diogo Niurka , Sifontes-Rodríguez Sergio , Aguirre-García María Magdalena , Escalona-Montaño Alma Reyna , Espinosa-Buitrago Teresa , Mondragón-Flores Ricardo , Mondragón-Castelán Mónica Edith , Meneses-Marcel Alfredo , Pérez-Olvera Ofelia , Sánchez-Almaraz Daniel Andrés , Perez-Castillo Yunierkis , Arán-Redó Vicente
Published: October 2024

Indazoles have previously been identified as molecules with antiprotozoal activity. In this study, we evaluate the in vitro activity of thirteen 3-alkoxy-1-benzyl-5-nitroindazole derivatives (series D) against L. amazonensis, L. infantum, and L. mexicana. In vitro, cytotoxicity against mouse peritoneal macrophages and growth inhibitory activity in promastigotes were evaluated for all compounds, and those showing adequate activity and selectivity were tested against intracellular amastigotes. Transmission and scanning electron microscopy were employed to study the effects of 3-alkoxy-1-benzyl-5-nitroindazole and 2-benzyl-5-nitroindazolin-3-one derivatives on promastigotes of L. amazonensis. Compounds NV6 and NV8 were active in the two life stages of the three species, with the latter showing the best indicators of activity and selectivity. 3-alkoxy-1-benzyl-5-nitroindazole derivatives (series D) showed in vitro activity comparable to that of amphotericin B against the promastigote stage of Leishmania spp. Two compounds were also found to be active the amastigote stage. Electron microscopy studies confirmed the antileishmanial activity of the indazole derivatives studied and support future research on this family of compounds as antileishmanial agents.
Genome-Wide Identification, Phylogenetic, and Expression Analysis of Jasmonate ZIM-Domain Gene Family in Medicago Sativa L.
Authors: Cui Jing , Jiang Xu , Li Yajing , Zhang Lili , Zhang Yangyang , Wang Xue , He Fei , Li Mingna , Zhang Tiejun , Kang Junmei
Published: October 2024

JASMONATE ZIM domain (JAZ) proteins, inhibitors of the jasmonic acid (JA) signaling pathway, are identified in different plants, such as rice and Arabidopsis. These proteins are crucial for growth, development, and abiotic stress responses. However, limited information is available regarding the JAZ family in alfalfa. This study identified 11 JAZ genes (MsJAZs) in the “Zhongmu No.1” reference genome of alfalfa. The physical and chemical properties, chromosome localization, phylogenetic relationships, gene structure, cis-acting elements, and collinearity of the 11 MsJAZ genes were subsequently analyzed. Tissue-specific analysis revealed distinct functions of different MsJAZ genes in growth and development. The expression patterns of MsJAZ genes under salt stress conditions were validated using qRT-PCR. All MsJAZ genes responded to salt stress, with varying levels of upregulation over time, highlighting their role in stress responses. Furthermore, heterogeneous expression of MsJAZ1 in Arabidopsis resulted in significantly lower seed germination and survival rates in OE-2 and OE-4 compared to the WT under 150 mM NaCl treatment. This study establishes a foundation for further exploration of the function of the JAZ family and provides significant insights into the genetic improvement of alfalfa.
Readdressing the Localization of Apolipoprotein E (APOE) in Mitochondria-Associated Endoplasmic Reticulum (ER) Membranes (MAMs): An Investigation of the Hepatic Protein–Protein Interactions of APOE with the Mitochondrial Proteins Lon Protease (LONP1), Mitochondrial Import Receptor Subunit TOM40 (TOMM40) and Voltage-Dependent Anion-Selective Channel 1 (VDAC1)
Authors: Rueter Johanna , Rimbach Gerald , Bilke Stephanie , Tholey Andreas , Huebbe Patricia
Published: October 2024

As a component of circulating lipoproteins, APOE binds to cell surface receptors mediating lipoprotein metabolism and cholesterol transport. A growing body of evidence, including the identification of a broad variety of cellular proteins interacting with APOE, suggests additional independent functions. Investigating cellular localization and protein–protein interactions in cultured human hepatocytes, we aimed to contribute to the elucidation of hitherto unnoted cellular functions of APOE. We observed a strong accumulation of APOE in MAMs, equally evident for the two major isoforms APOE3 and APOE4. Using mass spectrometry proteome analyses, novel and previously noted APOE interactors were identified, including the mitochondrial proteins TOMM40, LONP1 and VDAC1. All three interactors were present in MAM fractions, which we think initially facilitates interactions with APOE. LONP1 is a protease with chaperone activity, which migrated to MAMs in response to ER stress, displaying a reinforced interaction with APOE. We therefore hypothesize that APOE may help in the unfolded protein response (UPR) by acting as a co-chaperone in cooperation with LONP1 at the interface of mitochondria and ER membranes. The interaction of APOE with the integral proteins TOMM40 and VDAC1 may point to the formation of bridging complexes connecting mitochondria with other organelles.
Anti-Herpetic Activity of Killer Peptide (KP): An In Vitro Study
Authors: Sala Arianna , Ricchi Francesco , Giovati Laura , Conti Stefania , Ciociola Tecla , Cermelli Claudio
Published: October 2024

Antimicrobial peptides represent a promising alternative to traditional drugs in relation to cost, toxicity, and, primarily, the growing problem of drug resistance. Here, we report on the activity against HSV-1 and HSV-2 of a previously described wide-spectrum synthetic decapeptide, Killer Peptide (KP). As determined by plaque reduction assays, treatment with KP at 100 μg/mL resulted in a reduction in the viral yield titer of 3.5 Logs for HSV-1 and 4.1 Logs for HSV-2. Further evaluation of KP antiviral activity focused on the early stages of the virus replicative cycle, including the determination of the residual infectivity of viral suspensions treated with KP. A direct effect of the peptide on viral particles impairing virus absorption and penetration was shown. The toxicity profile proved to be extremely good, with a selectivity index of 29.6 for HSV-1 and 156 for HSV-2. KP was also active against acyclovir (ACV)-resistant HSV isolates, while HSV subcultures in the presence of sub-inhibitory doses of KP did not lead to the emergence of resistant strains. Finally, the antiviral action of KP proved to be synergistic with that of ACV. Overall, these results demonstrate that KP could represent an interesting addition/alternative to acyclovir for antiviral treatment.
Breath Analysis via Gas Chromatography–Mass Spectrometry (GC-MS) in Chronic Coronary Syndrome (CCS): A Proof-of-Concept Study
Authors: Lombardi Marco , Segreti Andrea , Miglionico Marco , Pennazza Giorgio , Tocca Lorenzo , Amendola Luca , Vergallo Rocco , Di Sciascio Germano , Porto Italo , Grigioni Francesco , Antonelli Incalzi Raffaele
Published: October 2024

Background: This proof-of-concept study aimed to assess the diagnostic potential of gas chromatography–mass spectrometry (GC-MS) in profiling volatile organic compounds (VOCs) from exhaled breath as a diagnostic tool for the chronic coronary syndrome (CCS). Methods: Exhaled air was collected from patients undergoing invasive coronary angiography (ICA), with all samples obtained prior to ICA. Post hoc, patients were divided into groups based on coronary lesion severity and indications for revascularization. VOCs in the breath samples were analyzed using GC-MS. Results: This study included 23 patients, of whom 11 did not require myocardial revascularization and 12 did. GC-MS analysis successfully classified 10 of the 11 patients without the need for revascularization (sensitivity of 91%), and 7 of the 12 patients required revascularization (specificity 58%). In subgroup analysis, GC-MS demonstrated 100% sensitivity in identifying patients with significant coronary lesions requiring intervention when the cohort was divided into three groups. A total of 36 VOCs, including acetone, ethanol, and phenol, were identified as distinguishing markers between patient groups. Conclusions: Patients with CCS exhibited a unique fingerprint of exhaled breath, which was detectable with GC-MS. These findings suggest that GC-MS analysis could be a reliable and non-invasive diagnostic tool for CCS. Further studies with larger cohorts are necessary to validate these results and explore the potential integration of VOC analysis into clinical practice.
Intracranial Aneurysms and Cerebral Small Vessel Disease: Is There an Association between Large- and Small-Artery Diseases?
Authors: Swiatek Vanessa M. , Schreiber Stefanie , Amini Amir , Hasan David , Rashidi Ali , Stein Klaus-Peter , Neyazi Belal , Sandalcioglu I. Erol
Published: October 2024

Background/Objectives: Intracranial aneurysms (IAs) may be connected to interactions between large and small intracranial vessels. We aimed to investigate the association between IAs and cerebral small-vessel disease (CSVD) and assess CSVD impact on IA patient management. Methods: This retrospective study analyzed clinical data and MRI features of CSVD in 192 subarachnoid hemorrhage (SAH) patients: 136 with incidental IA, 147 with severe CSVD without SAH/IA, and 50 controls without SAH, IA, or severe CSVD. MRI assessments followed the Standards for Reporting Vascular Changes on Neuroimaging (STRIVE), with a total burden of small-vessel disease (TBSVD) score calculated. Statistical analyses included forward selection and binary logistic regression. Results: TBSVD differed significantly across groups (p < 0.001), except between SAH and IA groups (p = 0.8). Controls had the lowest TBSVD (1.00; 1.22 ± 0.996), followed by SAH (2.00; 2.08 ± 1.013) and IA groups (2.00; 2.04 ± 1.141), with the highest in the CSVD group (1.00; 1.22 ± 0.996). White-matter hyperintensity (WMH) patterns varied with IA rupture status (p = 0.044); type A was prevalent in SAH patients and type D in the IA group. Incorporating MRI CSVD features and TBSVD into risk assessments did not enhance IA prediction or outcome models. Conclusions: IA patients exhibit a higher CSVD burden than controls, suggesting a link between small and large intracranial vessels. WMH patterns distinguish between ruptured and unruptured IA patients, offering potential markers for IA rupture risk assessment and signaling a paradigm shift in understanding IAs and CSVD.
Cardiovascular Risk across Glycemic Categories: Insights from a Nationwide Screening in Mongolia, 2022–2023
Authors: Batmunkh Nomuuna , Enkhtugs Khangai , Munkhbat Khishignemekh , Davaakhuu Narantuya , Enebish Oyunsuren , Dangaa Bayarbold , Luvsansambuu Tumurbaatar , Togtmol Munkhsaikhan , Bayartsogt Batzorig , Batsukh Khishigjargal , Tsedev-Ochir Tumur-Ochir , Yadamsuren Enkhtur , Khasag Altaisaikhan , Unurjargal Tsolmon , Byambasukh Oyuntugs
Published: October 2024

(1) Background: Diabetes mellitus is a significant risk factor for cardiovascular disease (CVD), a leading cause of death globally. Recent studies have highlighted the role of pre-diabetes, particularly impaired fasting glucose (IFG), in elevating CVD risk even before the onset of diabetes. The objective of this study was to assess cardiovascular disease (CVD) risk across specific glycemic categories, including normoglycemia, impaired fasting glucose (IFG), newly diagnosed diabetes, and long-standing diabetes, in a large Mongolian population sample. (2) Methods: This cross-sectional study utilized data from a nationwide health screening program in Mongolia between 2022 and 2023, involving 120,266 adults after applying inclusion criteria. The participants were categorized based on fasting plasma glucose levels (NGT): normoglycemia, IFG, newly diagnosed diabetes, and long-standing diabetes. CVD risk was assessed using WHO risk prediction charts, considering factors like age, blood pressure, smoking status, and diabetes status. (3) Results: CVD risk varied significantly with glycemic status. Among those with NGT, 62.9% were at low risk, while 31.2% were at moderate risk. In contrast, the IFG participants had 49.5% at low risk and 39.9% at moderate risk. Newly diagnosed diabetes showed 38.1% at low risk and 43.3% at moderate risk, while long-standing diabetes had 33.7% at low risk and 45.9% at moderate risk. Regression analysis indicated that glycemic status was independently associated with moderate to high CVD risk (OR in IFG: 1.13; 95% CI: 1.09–1.18), even after adjusting for age, gender, and central obesity. (4) Conclusions: This study emphasizes the need for early cardiovascular risk assessment and intervention, even in pre-diabetic stages like IFG.
Prolactin and Hyperprolactinaemia in Endometriosis-Related Infertility: Are There Clinically Significant Connections?
Authors: Kutlesic Ranko , Kutlesic Marija , Milosevic-Stevanovic Jelena , Vukomanovic Predrag , Stefanovic Milan , Mostic-Stanisic Danka
Published: October 2024

Endometriosis and hyperprolactinaemia are conditions that might lead to infertility as a consequence. The aim of this article was to present the current knowledge about possible relationships between prolactin/hyperprolactinaemia and endometriosis-related infertility. Experimental studies on local prolactin acting as cytokine and relationship of prolactin and endometriotic tissue, as well as clinical studies on hyperprolactinaemia and endometriosis-related infertility suggest the possible role of prolactin in endometriosis-related infertility, but final proof is still missing and the exact pathogenesis of infertility in such cases is still under investigation. Novel strategies in the treatment of endometriosis-related infertility, based on its connection with prolactin such as the use of prolactin receptor antibodies and prolactin receptor antagonists, are under investigation, but adequate clinical studies have yet to be undertaken.
Characterisation of the Mechanical Properties of Natural Fibre Polypropylene Composites Manufactured with Automated Tape Placement
Authors: Legenstein Alexander , Haiden Lukas , Feuchter Michael , Fauster Ewald
Published: October 2024

The integration of natural fibre thermoplastic composites, particularly those combining flax fibres with polypropylene, offers a promising alternative to traditional synthetic composites, emphasising sustainability in composite materials. This study investigates the mechanical properties of flax/polypropylene composites manufactured using flashlamp automated tape placement and press consolidation, individually and in combination. Tensile, compression, three-point bending, and double cantilever beam tests are utilised for comparing these manufacturing processes and the mechanical performance of the resulting composites. The microstructure of the tapes is investigated using cross-sectional microscopy, and the thermophysical behaviour is analysed utilising thermogravimetric analysis and differential scanning calorimetry. The temperature during placement is monitored using an infrared camera, and the pressure is mapped with pressure-sensitive films. The natural fibre tapes show a good aptitude for being manufactured with automated tape placement. The tensile performance of tapes manufactured with automated tape placement is close to that of press consolidated samples. Compression, flexural properties, and the mode I fracture toughness critical energy release rate all benefit from a second consolidation step.
Delayed Blastocyst Formation Reduces the Quality and Hatching Ability of Porcine Parthenogenetic Blastocysts by Increasing DNA Damage, Decreasing Cell Proliferation, and Altering Transcription Factor Expression Patterns
Authors: Sun Ling , Wang Yan , Yang Mo , Xu Zhuang-Ju , Miao Juan , Bai Ying , Lin Tao
Published: October 2024

The purpose of this study was to investigate the influence of blastocyst formation timing on the quality of porcine embryos derived from parthenogenetic activation. Newly formed blastocysts at days 6, 7, and 8 of culture [termed formation 6, 7, and 8 blastocysts (F6, F7, and F8 blastocysts)] were obtained, and a series of parameters related to the quality of blastocysts, including apoptosis incidents, DNA replication, pluripotent factors, and blastocyst hatching capacity, were assessed. Delayed blastocyst formation (F7 and/or F8 blastocysts) led to increased levels of ROS, DNA damage, and apoptosis while decreasing the mitochondrial membrane potential, DNA replication, Oct4 levels, and numbers of Sox2-positive cells. F7 blastocysts showed a significantly reduced hatching rate compared to F6 blastocysts; however, F8 blastocysts were unable to develop to the hatching stage. Collectively, our findings suggest a negative correlation between delayed blastocyst formation and blastocyst quality.
The Relationship of Achievement Goal Orientations and 21st Century Skills Acquisition with the Entrepreneurship of Pre-Service Teachers
Authors: Ertem Hasan Yücel
Published: October 2024

Teachers’ goal orientations and their adaptation to the 21st century determine many questions in teacher training and professional development. One of these aspects is entrepreneurship, such that the achievement goal orientations and 21st century skills of pre-service teachers have a positive role in entrepreneurship. The present study aimed to investigate the predictive role of the achievement goal orientations and 21st century skills of pre-service teachers in relation to entrepreneurship. To this end, a correlational design was conducted to analyze relationships between variables. The sample in the current study consisted of 282 pre-service teachers studying at a Faculty of Education in Anatolia Province, Turkey. The instruments were the Achievement Goal Orientations Scale, 21st Century Learning Skills Acquisition Scale, and The Scale of Entrepreneurship of College Students. The data obtained from these scales were analyzed using structural equation modeling. The results showed that the structural model of the relationships between achievement goal orientations, 21st century skills, and entrepreneurship had an adequate goodness of fit, so that most of the achievement goal orientations and 21st century skills of pre-service teachers predicted either directly or indirectly their entrepreneurship skills. The conclusion was that entrepreneurial education activities related to teacher entrepreneurship can be planned by considering 21st century skills and achievement goal orientations in teacher education.
The Effect of Correcting Neuromyths on Students’ and Teachers’ Later Reasoning
Authors: Lithander Marcus Per Gustaf , Geraci Lisa , Karaca Meltem , Hunsberger Renee
Published: October 2024

Students and educators sometimes hold beliefs about intelligence and learning that lack scientific support, often called neuromyths. Neuromyths can be problematic, so it is important to find methods to correct them. Previous findings demonstrate that textual refutations are effective for correcting neuromyths. However, even after correction, erroneous information may continue to influence reasoning. In three experiments, we investigated whether feedback could be used to update students’ and educators’ beliefs and influence their reasoning about neuromyths. Across all experiments, the results showed that both students and educators held erroneous beliefs about learning and memory that could be updated after receiving feedback. Feedback also increased students’, but not teachers’, reasoning accuracy. The results demonstrate that feedback can be used to update beliefs in neuromyths, but these beliefs may influence reasoning even after correction.
Structure and Evolution of Multi-Trend Faults in BZ19-6 Buried Hill of the Bohai Bay Basin, Eastern China
Authors: Lou Rui , Sun Yonghe , Jiang Fujie , Liu Yumin , Gao Tian
Published: October 2024

Defining the structure and evolution of multi-trend faults is critical for analyzing the accumulation of hydrocarbons in buried hills. Based on high-resolution seismic and drilling data, the structural characteristics and evolutionary mechanism of multi-trend faults were investigated in detail through the structural analysis theory and quantitative calculations of fault activity, allowing us to determine the implication that fault evolution exerts on hydrocarbon accumulation in the BZ19-6 buried hill. There are four kinds of strike faults developed on the buried hill: SN-, NNE-, NE–ENE-, and nearly EW-trending, which experienced the Mesozoic Indosinian, Yanshan, and Cenozoic Himalayan tectonic movements. During the Indosinian, the BZ19-6 was in a SN-oriented compressional setting, with active faults composed of SN-trending strike-slip faults (west branch of the Tanlu fault zone) and near EW-trending thrust faults (Zhang-peng fault zone). During the Yanshanian, the NNE-trending normal faults were formed under the WNW–ESE tensile stress field. Since the Himalayan period, the BZ19-6 buried hill has evolved into the rifting stage. In rifting stage Ⅰ, all of the multi-trend pre-existing faults were reactivated, and the EW-trending thrust faults became normal faults due to negative inversion. In rifting stage II, a large number of NE–ENE-trending normal faults were newly formed in the NW–SE-oriented extensional setting, which made the structure pattern more complicated. In rifting stage III, the buried hill entered the post-rift stage, with only part of the NNE- and NE–ENE-trending faults continuously active. Multi-trend faults are the result of the combination of various multi-phase stress fields and pre-existing structures, which have great influence on the formation of tectonic fractures and then control the distribution of high-quality reservoirs in buried hills. The fractures controlled by the NNE- and EW-trending faults have higher density and scale, and fractures controlled by NE–ENE trending faults have stronger connectivity and effectiveness. The superposition of multi-trend faults is the favorable distribution of high-quality reservoirs and the favorable accumulation area of hydrocarbon.
Analysis of Saturated Impulse for Square Plates under Flat Slamming Impact: Experimental and Numerical Investigation
Authors: Zhu Zhikui , Zhu Ling , Guo Kailing , Wang Xueliang
Published: October 2024

The saturated impulse is a special phenomenon in the dynamic plastic behavior of engineering structures under intensive pulse loading, such as slamming loading. In this study, slamming experiments were performed on steel plates to investigate their slamming pressure and dynamic plastic responses, as well as the saturation phenomenon, and elucidate the effect of the plate thickness and material properties on the dimensionless saturated deflection and saturated impulse in combination with the published test data. The results show that the dimensionless saturated deflection and saturated impulse of the test plates gradually increased as the dimensionless stiffness decreased. After being validated against the experimental results, a numerical method that considered the fluid–structure interaction (FSI) effect was then employed to provide comprehensive insight into the transient plastic responses and saturated impulse of the flat plates under slamming impact. Numerical simulations revealed that the compressed air layer always existed during the effective process of the flat slamming impact. Through the numerical prediction of the dynamic plastic deflection and slamming pulse loading, it was observed that the saturated impulse phenomenon always took place after the time instant of the peak value of the pressure pulse. Furthermore, the analysis of the saturated impulse based on the numerical simulations indicated that the saturation phenomenon was more likely to be achieved as the water impact velocity increased.
Corporate Governance and Financial Performance: Family Firms vs. Non-Family Firms
Authors: Mashele Audney , Mouton Marise , Pelcher Lydia
Published: October 2024

The essence of a family business captures the distinguishing factors differentiating them from non-family businesses. Among these factors, the constructs of family firms’ managing and governance elements are perceived differently by non-family firms. This is especially important in a developing country such as South Africa (SA) with many governance challenges. The objectives of this study were, first, to identify relationships among financial performance, corporate governance, and ownership concentrations of listed family and non-family businesses in SA. Next, a comparison was made between the different ownership structures. Secondary data were collected using purposive sampling from 2015 to 2019. These data were analysed using panel data analysis and descriptive statistics. The results show that family firms place a greater emphasis on ownership concentration, board size, and board gender diversity, which have a significant relationship with financial performance. Only board size was significant to financial performance for non-family firms. The results indicate that family businesses should appoint female family members as directors on their boards, given the significance of gender-diverse boards for financial performance. Non-family businesses should also consider having smaller boards. Theoretically, this study expands on the literature regarding family businesses in SA. However, the findings cannot be generalised due to a single industry being selected. This study should be replicated in different industries to compare the results.
The Integration of Radiomics and Artificial Intelligence in Modern Medicine
Authors: Maniaci Antonino , Lavalle Salvatore , Gagliano Caterina , Lentini Mario , Masiello Edoardo , Parisi Federica , Iannella Giannicola , Cilia Nicole Dalia , Salerno Valerio , Cusumano Giacomo , La Via Luigi
Published: October 2024

With profound effects on patient care, the role of artificial intelligence (AI) in radiomics has become a disruptive force in contemporary medicine. Radiomics, the quantitative feature extraction and analysis from medical images, offers useful imaging biomarkers that can reveal important information about the nature of diseases, how well patients respond to treatment and patient outcomes. The use of AI techniques in radiomics, such as machine learning and deep learning, has made it possible to create sophisticated computer-aided diagnostic systems, predictive models, and decision support tools. The many uses of AI in radiomics are examined in this review, encompassing its involvement of quantitative feature extraction from medical images, the machine learning, deep learning and computer-aided diagnostic (CAD) systems approaches in radiomics, and the effect of radiomics and AI on improving workflow automation and efficiency, optimize clinical trials and patient stratification. This review also covers the predictive modeling improvement by machine learning in radiomics, the multimodal integration and enhanced deep learning architectures, and the regulatory and clinical adoption considerations for radiomics-based CAD. Particular emphasis is given to the enormous potential for enhancing diagnosis precision, treatment personalization, and overall patient outcomes.
Is Thrombectomy Effective for Large Vessel Occlusion Stroke Patients with Mild Symptoms? Meta-Analysis and Trial Sequential Analysis
Authors: Chen Kuan-Chih , Li Te-Wei , Huang Ji-Kuan , Huang Cheng-Chieh , Zhang Siang-Yan , Chen Chih-Hung , Lin Zong-Syuan , Chen Po-Huang , Jhou Hong-Jie
Published: October 2024

Background: Endovascular treatment (EVT) is an established method for managing large vessel occlusion (LVO), but its efficacy in patients with mild stroke (National Institutes of Health Stroke Scale [NIHSS] score < 6) remains debated. Given the clinical problem of early neurological deterioration in approximately 10% of mild stroke patients, understanding the role of EVT in managing these patients is crucial. Our objective was to perform a meta-analysis with trial sequential analysis (TSA) focusing on mild stroke patients with LVO to determine whether EVT offers better outcomes than best medical therapy alone. Methods: A comprehensive search of PubMed, Cochrane, and Embase databases up to 12 December 2023 identified 14 retrospective and prospective cohort studies, including a total of 4436 patients with NIHSS scores less than 6 and presenting with LVO. Studies were categorized into crossover and non-crossover groups to prevent overestimation of the treatment effect. In the crossover group, patients initially treated with BMT were moved to EVT upon clinical deterioration. In the non-crossover group, patients remained in their initially assigned treatment. Meta-analysis and data extraction followed the Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines. The primary outcome was achieving an excellent functional outcome, defined as a modified Rankin scale (mRS) score of 0–1 at 3 months. Secondary outcomes included good (mRS 0–2) and favorable (mRS 0–3) functional outcomes. Safety outcomes were symptomatic intracerebral hemorrhage (sICH) and mortality at 3 months. Results: In the crossover group, EVT did not significantly improve excellent functional outcomes, and TSA results were inconclusive. Conversely, in the non-crossover group, EVT significantly improved the excellent functional outcome rates at 3 months (65.0% vs. 53.7%; OR 1.62; 95% CI 1.13 to 2.32), supported by TSA. EVT increased the risk of sICH in both crossover and non-crossover groups, while mortality rates did not significantly differ between EVT and BMT groups. Conclusions: Our research indicates that thrombectomy may not significantly help mild stroke patients in recovering functional status and could increase the risk of sICH. The disparity in results between crossover and non-crossover studies highlights the critical need for the prompt identification of patients at risk of early neurological deterioration to minimize negative outcomes. Additional randomized controlled trials are essential to optimize the application of EVT in this patient population.
The Effectiveness and Safety of Remdesivir Use in COVID-19 Patients with Neutropenia: A Retrospective Cohort Study
Authors: Liu Peng-Huei , Pan Ming-Wei , Huang Yan-Bo , Ng Chip-Jin , Chen Shou-Yen
Published: October 2024

Background: The COVID-19 pandemic poses severe risks for immunocompromised patients, especially those with neutropenia due to chemotherapy. This study evaluates the safety and effectiveness of remdesivir use in COVID-19 patients with neutropenia. Methods: This retrospective study used the Chang Gung Research Database (CGRD) and extracted data from 98,763 patients with COVID-19 diagnosed between April 2021 and September 2022. The patients were divided into groups based on their remdesivir use and the presence of neutropenia. The adverse effects of remdesivir and their outcomes were analyzed after propensity score matching. Results: We compared common adverse effects of remdesivir in neutropenic patients before and after a 5-day regimen. A slight decrease in heart rate was observed but lacked clinical significance. There were no significant differences observed in hemoglobin, liver function tests, and blood glucose levels. After propensity score matching of COVID-19 patients with neutropenia according to gender, age, dexamethasone use, oxygen use, MASCC score, and WHO ordinal scale, no significant differences were found in length of stay, intubation rate, or ICU admission rate between the matched patients. Conclusions: Our study found remdesivir to be safe for COVID-19 patients with neutropenia, with no common adverse reactions observed. However, its effectiveness for these patients remains uncertain.
Effects of Arthrospira platensis on Human Umbilical Vein Endothelial Cells
Authors: Krüger-Genge Anne , Harb Kudor , Braune Steffen , Jung Conrad H. G. , Westphal Sophia , Bär Stefanie , Mauger Olivia , Küpper Jan-Heiner , Jung Friedrich
Published: October 2024

Atherosclerosis is initiated by injury or damage to the vascular endothelial cell monolayer. Therefore, the early repair of the damaged vascular endothelium by a proliferation of neighbouring endothelial cells is important to prevent atherosclerosis and thrombotic events. Arthrospira platensis (AP) has been used as a dietary supplement, mainly due to its high content of vitamins, minerals, amino acids, and pigments such as chlorophylls, carotenoids, and phycocyanin, ingredients with antioxidant, anti-inflammatory, and anti-thrombotic properties. Therefore, in this prospective, placebo-controlled, data-driven, sample-size-estimated in vitro study, we tested whether an aqueous extract of AP at different concentrations (50, 100, and 200 µg/mL) had an effect on the different cellular parameters of human umbilical vein endothelial cells. Therefore, cell impedance measurement and cell proliferation were measured to investigate the monolayer formation. In addition, cell viability, integrity, and metabolism were analysed to evaluate singular cellular functions, especially the antithrombotic state. Furthermore, cell–cell and cell–substrate interactions were observed. The highest proliferation was achieved after the addition of 100 µg/mL. This was consistently confirmed by two independent optical experiments in cell cultures 48 h and 85 h after seeding and additionally by an indirect test. At this concentration, the activation or dysfunction of HUVECs was completely prevented, as confirmed by prostacyclin and interleukin-6 levels. In conclusion, in this study, AP induced a significant increase in HUVEC proliferation without inducing an inflammatory response but altered the hemostasiological balance in favour of prostacyclin over thromboxane, thereby creating an antithrombotic state. Thus, APE could be applied in the future as an accelerator of endothelial cell proliferation after, e.g., stent placement or atherosclerosis.
rac-2-(2′-Ferrocenyl-2′-hydroxy-n-propyl)-1,3-benzothiazole
Authors: Zhen Martin G. , May Kathleen L. , Gossage Robert A.
Published: October 2024

The synthesis and characterisation (UV-Vis, IR, HRESI-MS, 1H and 13C NMR spectroscopies, electrochemistry) is reported of the novel title material (1: alternatively named rac-1-(2′-benzothiazolyl)-2-ferrocenyl-2-propanol): a rare example of a ferrocenyl-benzothiazole hybrid species. Compound 1 is produced by the low temperature reaction of acetylferrocene (3) with a solution of the methyl anion derived via the deprotonation of 2-methyl-1,3-benzothiazole. The yield of 1 is moderate (34%) after purification and is an air and thermally stable solid under ambient conditions. Attempts to sublime 1, however, result in decomposition with one of the products being identified (NMR) as 3. The spectroscopic features of 1 are presented. Attempts to obtain suitable crystalline material of 1 for a single crystal X-ray diffraction study were unfortunately unsuccessful. Compound 1 also does not form stable coordination complexes with various metal salts (e.g., Ni[2+], Co[2+], etc.) under the conditions tested.
Comparative Study on Mechanical Properties and Microstructure Evolution of Mg-3Zn-1Mn/Sn Alloy through Ca-La-Ce Addition
Authors: Hu Ke , Tian Tingting , She Yunfeng , Guo Xiaoming , She Lixia , Huang Junjie , Huo Xiaomin , Liu Xiao , Xiong Zhaoting , Lu Chao
Published: October 2024

This study systematically investigates the influence of the composite addition of Ce, La, and Ca elements on the microstructure evolution and mechanical properties of Mg-3Zn-1Mn/Sn (wt.%) alloys. It indicates that the strength of Mg-Zn-Mn series alloys is superior to that of Mg-Zn-Sn series alloys, due to the stronger restriction of nanosized Mn particles on the recrystallization process and grain growth compared with Mg2Sn phases. The addition of the Ca-La-Ce elements significantly enhances the strength of the Mg-3Zn-1Sn alloy (YS increased by approximately 92.5%, UTS increased by approximately 29.2%, and EL decreased by nearly 52.2%), while for the Mg-3Zn-1Mn alloy, a balanced effect on both the strength and performance can be achieved. This difference mainly lies in the more pronounced refined effect on the grain size and the formation of a bimodal grain structure with strip-like un-DRXed grains and surrounding fine DRXed grains for the Mg-3Zn-1Sn alloy. In contrast, the addition of the Ca-La-Ce elements has a less obvious hindrance on the recrystallization process in the Mg-Zn-Mn series alloy, while significantly weakening the extrusion texture while refining the grains. Through in-depth characterization and experimental analysis, it is found that Sn and Ca can promote the formation of brittle and fine secondary phases. A nanoscale Sn phase (Mg2Sn phase) is more likely to accumulate at the grain boundaries, and the size of the nanoscale Ca2Mg6Zn3 in Mg-Zn-Mn series alloys is finer and more dispersed than that in Mg-Zn-Sn series alloys, thus strongly hindering recrystallization and refining the recrystallized structure of the alloy.
Hydration and Mechanical Properties of Cement Kiln Dust-Blended Cement Composite
Authors: Lee Woo-Seok , Choi Young-Cheol
Published: October 2024

This study aims to investigate the effects of cement kiln dust (CKD) on the hydration reactions and mechanical properties of cement, and to evaluate its potential for use as a supplementary cementitious material (SCM). The key variables are the CKD type and the replacement ratio. Cement paste and mortar specimens containing CKD were prepared to examine their effects on the cement hydration and mechanical properties. The effect on hydration was assessed using setting time measurements, heat of hydration tests, and thermogravimetric analyses (TG). In addition, compressive strength tests were conducted to evaluate the effect of CKD on the mechanical properties of the cement. The results indicated that CKD promoted early-age cement hydration and enhanced the early-age mechanical properties. However, owing to its lack of pozzolanic reactivity, it did not significantly affect long-term hydration. Given that the effects of CKD vary slightly depending on its chemical composition, careful consideration of CKD’s properties suggests that its potential use as an SCM is promising.
Convolutional Long Short-Term Memory Predictor for Collaborative Remotely Operated Vehicle Trajectory Tracking in a Leader–Follower Formation Subject to Communication and Sensor Latency in the Presence of External Disturbances
Authors: Pérez-Alvarado Milton Eduardo , Gómez-Espinosa Alfonso , González-García Josué , García-Valdovinos Luis Govinda , Salgado-Jiménez Tomás
Published: October 2024

Nowadays, collaborative operations between Remotely Operated Vehicles (ROVs) face considerable challenges, particularly in leader–follower schemes. The underwater environment imposes limitations on acoustic modems, leading to reduced transmission speeds and increased latency in ROV position and speed transmission. This complicates effective communication between the ROVs. Traditional methods, such as Recursive Least Squares (RLS) predictors and the Kalman filter, have been employed to address these issues. However, these approaches have limitations in handling non-linear patterns and disturbances in underwater environments. This paper introduces a Convolutional Long Short-Term Memory (ConvLSTM) predictor designed to enhance communication and trajectory tracking between ROVs in a leader–follower scheme. The proposed ConvLSTM aims to address the shortcomings of previous methods by adapting effectively to varying conditions, including ocean currents, communication delays, and signal interruptions. Simulations were conducted to evaluate ConvLSTM’s performance and compare it with other advanced predictors, such as Multilayer Perceptron (MLP) and Long Short-Term Memory (LSTM), under different conditions. The results demonstrated that ConvLSTM achieved a 13.9% improvement in trajectory tracking, surpassing other predictors in scenarios that replicate real underwater conditions and multi-vehicle communication. These results highlight ConvLSTM’s potential to significantly enhance the performance and stability of collaborative ROV operations in dynamic underwater environments.
Structural Design and Experimental Analysis of the Self-Balancing Lower Limb Exoskeleton Robot
Authors: Li Ming , Yin Meng , Chen Xu , Wu Xinyu
Published: October 2024

To facilitate walking rehabilitation training for individuals with lower limb paralysis, a self-balancing exoskeleton robot with 12 degrees of freedom was conceived. The principal structural design was conducted in line with the biomechanics of the human lower limbs, and a kinematic model was formulated. The stipulated gait was resolved by reverse kinematics in MATLAB to derive the joint angle actuation curves. These curves served as the motive input in ADAMS kinematic simulation experiments, yielding a gait trajectory with an error margin of less than 2 mm compared to the prearranged gait, which is within a reasonable range of deviation. Experiments involving walking with the exoskeleton were also executed. The analysis of the six-axis force sensor data from the sole demonstrated that the ground reaction force curve consistently remained within the bounds of the foot’s support area, substantiating the exoskeleton’s capability for stable ambulation with a load. The simulations and walking experiments together verified the soundness of the exoskeleton’s structural design.
Impact of Heat Treatment Conditions and Cold Plastic Deformation on Secondary Hardening and Performance of Cold Work Tool Steel X160CrMoV12
Authors: Bendikiene Regita , Kavaliauskiene Lina
Published: October 2024

In this study, the effect of the cold plastic deformation of a Bridgman anvil at room temperature on the hardness and wear resistance of X160CrMoV12 steel was investigated by utilizing the hardness test, X-ray diffraction (XRD), abrasive emery wear (AEMW) test, optical examination, and scanning electron microscopy (SEM). Three batches of samples were prepared for the experiment: I—as-hardened, II—after hardening with subsequent tempering at 600 °C for 1.5 h, and III—after hardening with subsequent plastic deformation. The hardening of the samples was performed at three temperatures: 1100 °C, 1150 °C, and 1200 °C. The highest content of retained austenite, as much as 69.02%, was observed during hardening at 1200 °C, while 17.36% and 38.14% were formed at lower temperatures, respectively. After tempering (Batch II), the content of residual austenite decreased proportionally by a factor of about seven for each hardening temperature. The effect of plastic deformation (Batch III) is observed, analyzing the hardness of the samples from the surface to the depth, reaching an average hardened depth of 0.08 mm. To evaluate the wear resistance, the surfaces of the three test batches were subjected to an abrasive emery wear test under a 5 N load. Hardened and plastically deformed samples showed higher wear resistance than hardened and tempered samples. The results confirmed that the optimal hardening temperature to achieve the maximum wear resistance of this steel is 1100 °C.
Numerical Investigation of Heterogeneous Calcite Distributions in MICP Processes
Authors: Wang Lingxiang , Chu Yajie , Wang Xuerui , Pan Pengzhi , Feng Dianlei
Published: October 2024

Microbially induced calcite precipitation (MICP) is a sustainable and environmentally friendly technology with applications in soil stabilization, concrete crack repair, and wastewater treatment. This study presents an improved Darcy-scale numerical model to simulate the MICP processes in heterogeneous porous media. It focuses on the effects of porosity heterogeneity, characterized by average porosity and correlation length, as well as injection strategies. Both average porosity and correlation length are critical factors influencing mass transport and calcite distribution during MICP treatment. An increase in average porosity leads to significant reductions in transport distance and total calcite mass. Notably, in the case of low averaged porosity, a larger correlation length results in more heterogeneous calcite distributions. However, there exists an upper threshold value of the initial averaged porosity (ϕ0=0.45) above which the heterogeneity of the calcite does not present clear dependence on the correlation length. Additionally, injection strategies significantly impact the consolidation effects. Compared to continuous injection, using the phased injection strategy can greatly improve the precipitated calcite area and mass due to its high utility and the efficiency of reactants.
Extraction of Rare Earth and CaF2 from Rare Earth Calcium Thermal Reduction Slag by Using CaO Roasting–Acid Leaching Method
Authors: Huang Jinqiu , Zhang Lizhi , Yu Wen , Chen Jiangan , Le Chengtao , Ren Sili
Published: October 2024

The rare earth calcium thermal reduction slag (RCS) generated during the production of heavy rare earth metal contains large amounts of rare earth and fluoride compounds. In this study, rare earth elements (REEs) and fluorine in the RCS were recovered by the CaO roasting–hydrochloric acid leaching method. Firstly, the thermodynamic feasibility of converting rare earth fluoride to rare earth oxides through CaO roasting was demonstrated. The influence of roasting conditions and leaching conditions on the leaching rate of the REEs was investigated. Optimal results, a 95.48% leaching rate of the REEs, were obtained under the following conditions: a CaO dosage of 15%, a roasting temperature of 1000 °C, and a roasting duration of 90 min. XRD, SEM, and EDS results revealed that during the calcination process, the REEs present in fluorite (CaF2) in isomorphic form were transformed into acid-soluble rare earth oxides; furthermore, the rare earth metallic in the RCS remained unchanged even after roasting. In the leaching process, rare earth metals and rare earth oxides are efficiently extracted, while CaF2 rarely dissolves. The leaching slag contained 97.31% CaF2 with a F recovery of 96.92%. The kinetics of the rare earth leaching process was analyzed, and the results show that the three-dimensional diffusion control at the phase interface of the kinetic model best fits the process. The calculated apparent activation energy for the leaching rate of REEs is 20.869 kJ/mol. Therefore, efficient comprehensive recovery of rare earth and fluorite from RCS can be achieved by using the CaO roasting–hydrochloric acid leaching method.
Electrochemical Nickel-Catalyzed Synthesis of Unsymmetrical Diorganyl Selanes from Diaryl Diselanes and Aryl and Alkyl Iodides
Authors: Queder Jona , Hilt Gerhard
Published: October 2024

The synthesis of unsymmetrical diorganyl selanes was accomplished under electrochemical conditions in an undivided cell utilizing a magnesium cathode and a carbon anode made out of aryl and alkyl iodides and diselanes. This electrochemical cross-electrophile coupling (eXEC) was accomplished using a simple nickel catalyst formed in situ out of Ni(acac)2 and 2,2′-bipyridine in DMF at ambient temperatures. The reaction showed good functional group compatibility, and heteroaryl iodides, such as thiophene or pyridine derivatives, were well accepted.