bluuebunny/crossref_metadata_2025 huggingface dataset:
Posttraumatic Stress Symptoms Increase the Efficiency of Memory Functioning for Trauma-Related Information
Authors: Tudorache Andrei-Cristian , El-Hage Wissam , Clarys David
Published: February 2022

Objective:

The present study explored the resources reallocation explanation for memory biases in posttraumatic stress disorder (PTSD), whereby a preferential allocation of cognitive resources to the processing of threatening stimuli could result in both improvements in their memorization and deficits for other types of information.

Method:

To this end, 25 participants presenting significant symptoms of PTSD (i.e., total PCL-5 score ≥33) and 32 participants presenting low levels of symptoms (i.e., total PCL-5 score <20) took part in a Remember/Know recognition procedure associated with a dual-task encoding of positive, neutral, negative, and trauma-related words. In order to manipulate the availability of cognitive resources, the encoding of each word was associated with a simultaneous encoding of series of letters and numbers.

Results:

Results replicated the increased production of Remember recognitions for trauma-related words in participants with significant PTSD symptoms. However, the dual-task load only impaired remember recognitions for non-trauma-related words.

Conclusions:

Contrary to expectations, these findings suggest that the encoding of trauma-related information in PTSD is relatively independent from the availability of cognitive resources. Thus, rather than reflecting an increased allocation of cognitive resources to the processing of threatening information, memory biases in PTSD appeared to be supported by an enhanced efficiency of their processing.
The Travels of Lao Can as a book of prophecy
Authors: Feng Xiangjun
Published: February 2022

This article interrogates the early reception history of the late Qing Chinese novel The Travels of Lao Can authored by Liu E (1857–1909). It argues that for decades this novel was widely read as a book of prophecy that revealed the future of China based on traditional Chinese occult wisdom. It was this alleged mystique, instead of its literary merits, that accounted for the high popularity of the text in the late Qing and Republican book market. This argument is predicated upon the discovery and reappraisal of a 1916 “fake edition” of the book until now unremarked in Chinese literary historiography. Investigating this forgery reveals that it was arguably the most widely circulated edition of The Travels of Lao Can in the Republican period. Bound with a newly fabricated sequel that explicitly manifested its “divinatory efficacy”, this edition greatly shaped the popular reception of the text as a book of prophecy. Revisiting this “fake edition” and the forgotten stories behind it not only enables the rereading of The Travels of Lao Can in its full complexity, but also points to deeper questions about the relationship between print culture, literary consumption, and the production of occult knowledge in China's modern transition.
‘You know what, I’m in the trend as well’: understanding the interplay between digital and real-life social influences on the food and activity choices of young adults
Authors: Leu Jodie , Tay Zoey , van Dam Rob M , Müller-Riemenschneider Falk , Lean Michael EJ , Nikolaou Charoula Konstantia , Rebello Salome A
Published: February 2022

Objective:

To understand young adults’ perceptions of online and real-life social influences on their food and activity choices.

Design:

A qualitative study involving 7 focus groups. Thematic analysis using both deductive and inductive techniques were performed.

Setting:

A polytechnic and a university in Singapore.

Participants:

A total of 46 full-time students, 19–24 years of age.

Results:

Participants revealed that social media meets multiple needs, contributing to its ubiquitous use and facilitating content spread between social networks. Food-related content shared on social media were mostly commercial posts, marketing foods and eateries showcasing price promotions, emphasising sensory properties of foods or creating narratives that activated trends. Subsequently, real-life social activities frequently revolve around marketed foods that were not necessarily healthy. In contrast, physical activity posts were rarely being followed up in real life. Portrayals describing a toxic gym culture could contribute to negative perceptions of peers’ physical activity posts and a disinclination towards sharing such posts. Participants expressed that close, supportive social networks in real life strongly influenced initiating and maintaining healthy lifestyles. However, in a society that highly values academic achievements, participants prioritised studying and socialising over healthy eating and physical activity.

Conclusions:

Overall, our findings reveal that virtual and real-life social influences have complex interactions affecting Asian young adults’ behavioural choices and should be considered when designing interventions for this group. Regulations related to the digital marketing of unhealthy food, and improving the availability, accessibility and affordability of healthier food options, particularly in the foodservice sector, would be of value to consider.
How Americans eat red and processed meat: an analysis of the contribution of thirteen different food groups
Authors: Frank Sarah M , Taillie Lindsey Smith , Jaacks Lindsay M
Published: February 2022

Objective:

Dietary patterns characterised by high intake of red and processed meat are associated with detrimental health and environmental outcomes. To better understand how Americans consume red and processed meat, this study examined the food groups that are the greatest contributors to red and processed meat intake in US diets.

Design:

Cross-sectional analysis of total red and processed meat, unprocessed red meat and processed meat using data from the National Health and Nutrition Examination Survey (2015–2016 and 2017–2018). Items containing red or processed meat were classified into thirteen mutually exclusive food groups. For highly consumed food groups (≥10 % of meat intake), contribution to meat intake was further assessed by source, sex, income and education.

Setting:

Nationally representative sample of the US population.

Participants:

Teens (aged 12–19 years) and adults (aged ≥20 years) who reported meat consumption (n 8178).

Results:

Meat mixed dishes (18·6 % (95 % CI 16·2, 20·9)), burgers (17·3 % (95 % CI 15·3, 19·3)) and beef excluding ground (17·0 % (95 % CI 13·8, 20·1)) were the top contributors to unprocessed red meat intake. For processed meat, four food groups made up about four-fifths of total intake: cold cuts and cured meats (37·7 % (95 % CI 34·6, 40·8)), sausages and frankfurters (20·3 % (95 % CI 18·6, 22·0)), bacon (14·0 % (95 % CI 12·3, 15·6)) and pizza (10·1 % (95 % CI 8·7, 11·5)). Fast-food restaurants were the top source for burgers and pizza, whereas stores were the top source for all other highly consumed food groups. Few differences were seen in patterns of intake by sociodemographic characteristics.

Conclusions:

No single food group accounts for a majority of meat intake in the USA. Many behaviour change opportunities for healthier, more sustainable substitutions exist.
The Hands Off Ethiopia campaign, racial solidarities and intercolonial antifascism in South Asia (1935–36)
Authors: Buelli Arlena
Published: February 2022

The transnational campaign against the Italian invasion of the Ethiopian Empire (1936–36) has been widely acknowledged as a turning point for antiracist and anticolonial political organizing in the African continent and diaspora. This article seeks to reconstruct the South Asian participation in the Hands Off Ethiopia protests, to expand historical knowledge of the early-twentieth-century development of Afro-Asian solidarity ties as well as the intersection of anti-Fascist and anticolonial struggles. It examines the institutional responses to the invasion on the part of the Indian Legislative Assembly, and a series of demonstrations, local meetings and boycotts whose implications reverberated in the local and international press as well as in the concerns of British colonial authorities. As will be argued, this mobilization was fueled by feelings of racial solidarity, anti-imperialist analyses, anti-caste critiques, scriptural interpretations and religious universalisms.
Rapid photosynthetic and physiological response of 2,4-D–resistant Sumatran fleabane (Conyza sumatrensis) to 2,4-D as a survival strategy
Authors: Leal Jéssica F. L. , Souza Amanda dos S. , Borella Junior , Araujo André Lucas S. , Langaro Ana Claudia , Alves Monique M. , Ferreira Luana Jéssica S. , Morran Sarah , Zobiole Luiz H. S. , Lucio Felipe R. , Machado Aroldo F. L. , Gaines Todd A. , Pinho Camila F. de
Published: February 2022

In this work, we evaluated the short time-induced oxidative stress–mediated rapid metabolic and physiological responses of resistant and susceptible Sumatran fleabane [Conyza sumatrensis (Retz.) E. Walker; syn.: Erigeron sumatrensis Retz.] to 2,4-D herbicide. Under fixed conditions (25 C and 65 ± 5% relative humidity), we assayed injury symptoms, chlorophyll a fluorescence, and antioxidative systems of biotypes both resistant and susceptible to 2,4-D (1,005 g ae ha−1). Under 15 versus 25 C temperatures and light and dark conditions, oxidative stress–mediated damage was assayed on plants that received 2,4-D herbicide applications. The injury symptoms observed in the 2,4-D–resistant biotype were rapid necrosis in leaves within 30 min, with the reestablishment of normal growth within 1 to 2 wk after 2,4-D treatment. The basal antioxidant enzyme activities of superoxide dismutase, catalase, and ascorbate peroxidase were greater in the resistant than in the susceptible biotype, although the activities of all enzymes generally did not differ between untreated and treated in the resistant biotype. The resistant biotype showed great reduction (at 1 and 4 h after application) in the photosynthetic electron transport chain performance index, while these metabolic changes were only detected after 4 h in the susceptible biotype. The resistant biotype recovered from the foliar damage 1 to 2 wk after 2,4-D application, while the susceptible biotype was controlled. The production of H2O2 was responsive to temperature and increased more rapidly in the 2,4-D–resistant biotype than in the susceptible one at both 15 and 25 C; however, there was a greater increase at 25 C in the resistant biotype. H2O2 production was not light dependent in 2,4-D–resistant C. sumatrensis, with increases even under dark conditions. The 2,4-D–resistant biotype showed rapid photosynthetic damage, possibly due to the rapid necrosis and leaf disruption, and increased H2O2 content compared with the susceptible biotype.
Wissenschaftliches Arbeiten in der Pflege
Authors: Panfil Eva
Published: February 2022

Das erfolgreiche Lehrbuch hat sich innerhalb kürzester Zeit als Standardwerk etabliert. Es fasst alle relevanten Informationen über wissenschaftliches Arbeiten für Pflegende kurz und konkret zusammen. Verständlich wird erklärt, was wissenschaftliches Arbeiten in der Pflege bedeutet, mit welchen Techniken gearbeitet wird und welche Produkte als Ergebnis wissenschaftlichen Arbeitens entstehen.Neben einer Einführung in wissenschaftliches Arbeiten beschreiben die AutorInnen konkret, wie man kritisch denkt, englische Texte liest und schreibt, gut zuhört, effektiv mitschreibt, klug fragt, klar argumentiert, gewinnend referiert, auf den Punkt zusammenfasst, anschaulich visualisiert, effektiv Literatur recherchiert, bibliografiert und verwaltet, korrekt zitiert und verständlich schreibt. Als Produkte wissenschaftlichen Arbeitens zeigen die AutorInnen, wie man Themen findet, ein Exposee oder einen Projektplan schreibt, ein wissenschaftliches Manuskript verfasst, Artikel einreicht, ein Buch rezensiert, einen Vortrag hält, ein Poster präsentiert, Haus- und Abschlussarbeiten sowie Fachbuchbeiträge und Fachbücher verfasst. Die 4. Auflage wurde bzgl. der bestehenden Themen Literaturrecherche, Bibliografieren und Zitieren mit den neuen APA-7-Richtlinien sowie Publizieren in englischer Sprache und wissenschaftlichem Fehlverhalten vertieft und erweitert. Zitate aus dem BuchManieren: „Man kann es nicht deutlich genug sagen: Rauchen gefährdet Ihre Gesundheit, ein Plagiat Ihre Karriere.“ Mitschreiben: „Schreiben ist Denken auf dem Papier.“ (Baumann)
ADHD Symptoms and Health-Related Quality of Life of Adolescents and Young Adults
Authors: Krauss Annette , Schellenberg Claudia
Published: February 2022

Abstract. Background: Adolescence and young adulthood present particularly challenging periods for individuals affected by attention-deficit hyperactivity disorder (ADHD) symptoms. However, unlike childhood ADHD, ADHD among adolescents and young adults has been studied less frequently. A concept that can be used to characterize the experiences of individuals with ADHD symptoms is health-related quality of life (HRQoL), which encompasses well-being in various dimensions. Aims: The present study used a school-based sample in the German-speaking part of Switzerland to investigate the association between ADHD symptoms and HRQoL of adolescents and young adults. Method: A total of 907 individuals aged 14–24 years ( M = 17.69; SD = 1.66; 58.9% female) were surveyed regarding ADHD symptoms and HRQoL. Results: Multiple hierarchical regression analyses disclosed that ADHD symptoms were negatively related to all subdimensions of HRQoL. Moreover, adolescents with clinically significant or subclinical ADHD symptoms showed lower values in all examined dimensions – physical, emotional, self-esteem, family, friends/peers, and school/education – than adolescents with unremarkable ADHD symptoms. An exception was the well-being concerning friends/peers. Limitations: The present study used a cross-sectional design and a selective sample of school classes. ADHD symptoms were assessed using self-reports without any diagnostic judgment. Conclusion: The results illustrate the comprehensive effects of ADHD symptoms on well-being during adolescence and young adulthood. They highlight the importance of focusing more on ADHD during these stages of age, also considering individuals with symptoms in the subclinical range.
Tracing Atmospheric Anthropogenic Black Carbon and Its Potential Radiative Response Over Pan‐Third Pole Region: A Synoptic‐Scale Analysis Using WRF‐Chem
Authors: Rai Mukesh , Kang Shichang , Yang Junhua , Chen Xintong , Hu Yuling , Rupakheti Dipesh
Published: February 2022

The Pan‐Third Pole contains the largest number of glaciers outside the polar region that plays a crucial role in atmospheric circulation and the hydrological cycle. However, this pristine region has undergone rapid change through complex interactions including the black carbon (BC) enhanced warming effect and glacier melting. Study shows, Weather Research and Forecasting coupled with Chemistry (WRF‐Chem) simulation is able to capture distinctive seasonal variability of BC. The result from our sensitivity experiments revealed that South Asia (SA; 60.7%) and East Asia (EA; 32.9%) contributed more toward the Tibetan Plateau (TP). Our analysis on aerosol‐boundary feedback interaction revealed BC expand planetary boundary layer height by 5.0% and 4.8% over SA and EA, respectively, which facilitates BC dispersion and transportation. Whereas, we also found that under the influence of different wind regimes the significant BC transport flux aloft over the TP and the upper troposphere and lower stratosphere. Additionally, mountain‐valley channel and synoptic and local meteorological processes also facilitated BC transport to the TP. This study also evaluated the effect of BC on direct radiative forcing and calculated subsequent temperature changes. A strong dimming effect of BC corroborated with the following negative surface temperature changes. However, enhanced BC concentration during winter and spring caused the increase in temperature over the TP. Here, the WRF‐Chem model, synergy on aerosol‐boundary feedback, BC transport flux, and source‐receptor methods confirmed the significant BC contribution and transportation, and notable BC‐induced warming over TP. Such trans‐Himalayan BC transport and associated warming could grim glacier melt and water availability in the region.
Modeling Low‐Frequency Radio Emissions From Terrestrial Gamma Ray Flash Sources
Authors: Berge Nini , Celestin Sebastien , Garnung Matthieu B. , Xu Wei , Marshall Robert A. , Cummer Steven A.
Published: February 2022

Relativistic runaway electron avalanches (RREAs) occur when electrons in electric fields in air reach energies above which they gain more energy from the electric field than they lose to collisions with the surrounding atmosphere. RREAs are known to happen in the electric fields in thunderstorms, and are considered to be the mechanism responsible for producing Terrestrial Gamma‐ray Flashes (TGFs). As RREAs propagate, they leave a trail of low‐energy electrons and positive and negative ions behind. These populations of charged particles will carry currents as they move in the thunderstorm electric field. In the present work, we model the charged species left behind by the propagating RREA, and the resulting radio emissions in the context of injection of thermal runaway seed electrons by a leader. We find that for certain initial conditions, these radio emissions match the slow low‐frequency (LF) pulses that have previously been observed concurrently with TGFs. This confirms that the slow LF pulses are likely generated directly by the TGF source itself, as has been previously suggested using a different TGF production model. Slow LF pulses may therefore potentially be used to infer characteristic properties of TGF sources.
The Contribution of Convection to the Stratospheric Water Vapor: The First Budget Using a Global Storm‐Resolving Model
Authors: Dauhut Thibaut , Hohenegger Cathy
Published: February 2022

The deepest convection on Earth injects water in the tropical stratosphere, but its contribution to the global stratospheric water budget remains uncertain. The Global Storm‐Resolving Model ICOsahedral Non‐hydrostatic is used to simulate the moistening of the lower stratosphere for 40 days during boreal summer. The decomposition of the water vapor budget in the tropical lower stratosphere (TLS, 10°S–30°N, and 17–20 km altitude) indicates that the average moistening (+21 Tg) over the simulated 40‐day period is the result of the combined effect of the vertical water vapor transport from the troposphere (+27 Tg), microphysical phase changes and subgrid‐scale transport (+2 Tg), partly compensated by horizontal water vapor export (−8 Tg). The very deep convective systems, explicitly represented thanks to the employed 2.5 km grid spacing of the model, are identified using the very low Outgoing Longwave Radiation of their cold cloud tops. The water vapor budget reveals that the vertical transport, the sublimation and the subgrid‐scale transport at their top contribute together to 11% of the water vapor mass input into the TLS.
Sediment Trap Study Reveals Dominant Contribution of Metazoans and Dinoflagellates to Carbon Export and Dynamic Impacts of Microbes in a Subtropical Marginal Sea
Authors: Lin Senjie , Li Tangcheng , Yuan Huatao , Li Hongfei , Yu Liying , Zhuang Yunyun , Li Ling , Huang Bangqin , Liu Guangxing
Published: February 2022

Flux and efficiency of carbon export in the ocean has been widely studied using sediment traps, but due to limitations of traditional methods, differential contribution of different organisms is poorly understood. Here, we used DNA metabarcoding to document taxonomic composition for sediment traps deployed at a continental shelf (C6) and a continental slope (C9) site in the South China Sea. The results indicated that metazoans (mainly copepods) and dinoflagellates were the most dominant contributors overall, while dinoflagellates, diatoms, and haptophytes dominated the photosynthetic assemblage. For prokaryotes, Gammaproteobacteria dominated all samples. Furthermore, comparing the trap flora to overlying plankton revealed that metazoans and Polycystinea from Eukaryota and Gammaproteobacteria and Bacteroidetes from Prokaryota were enriched in the trap at both stations whereas haptophytes and Dinophyceae were consistently attenuated during sinking. In addition, Oceanospirillaceae and Rhodobacteraceae exhibited negative correlations with dinoflagellates, and based on overlying plankton metatranscriptomes, were most transcriptionally active lineages for metabolizing labile and semi‐labile organic carbon, potentially contributing to the Martin's decay of sinking particulate organic carbon. Using DNA and RNA sequencing technologies, this study provides novel insights into the lineage‐specific contribution and dynamic impacts of microbes to carbon export in a subtropical marginal sea.
Global metrics on ocular biometry: representative averages and standard deviations across ten countries from four continents
Authors: Ganesh Durga , Lin Shawn R.
Published: February 2022

Background/Objectives

We provide global averages and standard deviations for ocular biometry—axial length (AL), corneal radius of curvature (CR), anterior chamber depth (ACD), lens thickness (LT), white to white (WTW), and central corneal thickness (CT). We hope a better understanding of normal and abnormal values will help clinicians gain further insight into their surgical outcomes, especially for off-target eyes.

Subjects/Methods

We searched the MEDLINE database using keywords “axial length, corneal power, anterior chamber depth, lens thickness, white to white, and corneal thickness.” We included studies that reported averages and standard deviations on eye biometry for at least 1300 eyes. Global weighted averages and standard deviations were calculated using the Cochrane method.

Results

Fourteen studies were included, originating from Asia (Japan, Singapore, Myanmar, Iran, South Korea, China), Europe (Germany, United Kingdom, Portugal), Australia, and North America (United States). Global ocular biometry metrics were: AL—23.49 mm ± 1.35 mm, CR—7.69 mm ± 0.28 mm, ACD—3.10 mm ± 0.47 mm, WTW—11.80 mm ± 0.42 mm, LT—4.37 mm ± 0.43 mm, and CT—544 μm ± 38 μm. Total eyes per value ranged from 19,538 to 90,814.

Conclusions

We report global ocular biometry averages and standard deviations. No eyes were from studies in Africa or South America, highlighting the need to publish eye biometry data from these continents. We hope that promoting a deeper understanding of biometry values will help clinicians gain insight into surgical outcomes and drive innovations in lens calculations.
Global and transcription-coupled repair of 8-oxoG is initiated by nucleotide excision repair proteins
Authors: Kumar Namrata , Theil Arjan F. , Roginskaya Vera , Ali Yasmin , Calderon Michael , Watkins Simon C. , Barnes Ryan P. , Opresko Patricia L. , Pines Alex , Lans Hannes , Vermeulen Wim , Van Houten Bennett
Published: February 2022

UV-DDB, consisting of subunits DDB1 and DDB2, recognizes UV-induced photoproducts during global genome nucleotide excision repair (GG-NER). We recently demonstrated a noncanonical role of UV-DDB in stimulating base excision repair (BER) which raised several questions about the timing of UV-DDB arrival at 8-oxoguanine (8-oxoG), and the dependency of UV-DDB on the recruitment of downstream BER and NER proteins. Using two different approaches to introduce 8-oxoG in cells, we show that DDB2 is recruited to 8-oxoG immediately after damage and colocalizes with 8-oxoG glycosylase (OGG1) at sites of repair. 8-oxoG removal and OGG1 recruitment is significantly reduced in the absence of DDB2. NER proteins, XPA and XPC, also accumulate at 8-oxoG. While XPC recruitment is dependent on DDB2, XPA recruitment is DDB2-independent and transcription-coupled. Finally, DDB2 accumulation at 8-oxoG induces local chromatin unfolding. We propose that DDB2-mediated chromatin decompaction facilitates the recruitment of downstream BER proteins to 8-oxoG lesions.
Protease activities of vaginal Porphyromonas species disrupt coagulation and extracellular matrix in the cervicovaginal niche
Authors: Lithgow Karen V. , Buchholz Vienna C. H. , Ku Emily , Konschuh Shaelen , D’Aubeterre Ana , Sycuro Laura K.
Published: February 2022

Porphyromonas asaccharolyticaandPorphyromonas uenonisare common inhabitants of the vaginal microbiome, but their presence has been linked to adverse health outcomes for women, including bacterial vaginosis and preterm birth. However, little is known about the pathogenesis mechanisms of these bacteria. The related oral opportunistic pathogen,Porphyromonas gingivalis, is comparatively well-studied and known to secrete numerous extracellular matrix-targeting proteases. Among these are the gingipain family of cysteine proteases that drive periodontal disease progression and hematogenic transmission to the placenta. In this study, we demonstrate that vaginalPorphyromonasspecies secrete broad-acting proteases capable of freely diffusing within the cervicovaginal niche. These proteases degrade collagens that are enriched within the cervix (type I) and chorioamniotic membranes (type IV), as well as fibrinogen, which inhibits clot formation. Bioinformatic queries confirmed the absence of gingipain orthologs and identified five serine, cysteine, and metalloprotease candidates in each species. Inhibition assays revealed that each species’ proteolytic activity can be partially attributed to a secreted metalloprotease with broad substrate specificity that is distantly related to theP. gingivalisendopeptidase PepO. This characterization of virulence activities in vaginalPorphyromonasspecies highlights their potential to alter the homeostasis of reproductive tissues and harm human pregnancy through clotting disruption, fetal membrane weakening, and premature cervical remodeling.
Universal relation for life-span energy consumption in living organisms: Insights for the origin of aging
Authors: Escala Andrés
Published: February 2022

Metabolic energy consumption has long been thought to play a major role in the aging process (Pearl, The rate of living. University of London Press, London, 1928). Across species, a gram of tissue expends approximately the same amount of energy during the lifespan on average (Speakman, J Exp Biol 208:1717–1730, 2005). Energy restriction has also been shown to increase the maximum lifespan (McCay et al. J Nutr 10:63–79, 1935) and to retard age-associated changes (Weindruch and Walford, The retardation of aging and disease by dietary restriction. CC Thomas, Springfield, 1988). However, there are significant exceptions to universal energy consumption during the lifespan, mainly found by interclass comparison (Ramsey et al. Free Rad Biol Med 29:946–968, 2000; Atanasov, Trakia J Sci 10(3):1–14, 2012). Here, we present a universal relation that relates lifespan energy consumption to several physiological variables, such as body mass, temperature and the ratio of heart rate to respiratory rate, which have been shown to be valid for$$\sim 300$$∼300species representing different classes of living organisms, from unicellular organisms to the largest mammals. This relation has an average scattered pattern restricted to factors of 2, with 95% ($$2-\sigma$$2-σ) of the organisms having departures of less than a factor of$$\pi$$πfrom the relation, despite the difference of$$\sim 20$$∼20orders of magnitude in body mass, reducing any possible interclass variation in the relation to only a geometrical factor. This result can be interpreted as supporting evidence for the existence of an approximately constant total number$${\mathrm{N}}_{{\mathrm{r}}} \sim 10^8$$Nr∼108of respiration cycles per lifetime for all organisms studied, effectively predetermining the extension of life through the basic energetics of respiration (quantified by$${\mathrm{t}}_{{\mathrm{life}}} = \mathrm{N}_\mathrm{r}/\mathrm{f}_{{\mathrm{resp}}}$$tlife=Nr/fresp); this is an incentive to conduct future studies on the relation of such a constant number$${\mathrm{N}}_{\mathrm{r}}$$Nrof cycles per lifetime due to the production rates of free radicals and oxidants or alternative mechanisms, which may yield definite constraints on the origin of aging.
Laser fabrication and evaluation of holographic intrinsic physical unclonable functions
Authors: Anastasiou Aggeliki , Zacharaki Evangelia I. , Tsakas Anastasios , Moustakas Konstantinos , Alexandropoulos Dimitris
Published: February 2022

Optical Physical Unclonable Functions (PUFs) are well established as the most powerful anticounterfeiting tool. Despite the merits of optical PUFs, widespread use is hindered by existing implementations that are complicated and expensive. On top, the overwhelming majority of optical PUFs refer to extrinsic implementations. Here we overcome these limitations to demonstrate for the first time strong intrinsic optical PUFs with exceptional security characteristics. In doing so, we use Computer-Generated Holograms (CGHs) as optical, intrinsic, and image-based PUFs. The required randomness is offered by the non-deterministic fabrication process achieved with industrial friendly, nanosecond pulsed fiber lasers. Adding to simplicity and low cost, the digital fingerprint is derived by a setup which is designed to be adjustable in a production line. In addition, we propose a novel signature encoding and authentication mechanism that exploits manifold learning techniques to efficiently differentiate data reconstruction-related variation from counterfeit attacks. The proposed method is applied experimentally on silver plates. The robustness of the fabricated intrinsic optical PUFs is evaluated over time. The results have shown exceptional values for robustness and a probability of cloning up to $$10^{-14}$$

10

-
14

, which exceeds the standard acceptance rate in security applications.
Spatiotemporal dynamics in spiking recurrent neural networks using modified-full-FORCE on EEG signals
Authors: Ioannides Georgios , Kourouklides Ioannis , Astolfi Alessandro
Published: February 2022

Methods on modelling the human brain as a Complex System have increased remarkably in the literature as researchers seek to understand the underlying foundations behind cognition, behaviour, and perception. Computational methods, especially Graph Theory-based methods, have recently contributed significantly in understanding the wiring connectivity of the brain, modelling it as a set of nodes connected by edges. Therefore, the brain’s spatiotemporal dynamics can be holistically studied by considering a network, which consists of many neurons, represented by nodes. Various models have been proposed for modelling such neurons. A recently proposed method in training such networks, called full-Force, produces networks that perform tasks with fewer neurons and greater noise robustness than previous least-squares approaches (i.e. FORCE method). In this paper, the first direct applicability of a variant of the full-Force method to biologically-motivated Spiking RNNs (SRNNs) is demonstrated. The SRNN is a graph consisting of modules. Each module is modelled as a Small-World Network (SWN), which is a specific type of a biologically-plausible graph. So, the first direct applicability of a variant of the full-Force method to modular SWNs is demonstrated, evaluated through regression and information theoretic metrics. For the first time, the aforementioned method is applied to spiking neuron models and trained on various real-life Electroencephalography (EEG) signals. To the best of the authors’ knowledge, all the contributions of this paper are novel. Results show that trained SRNNs match EEG signals almost perfectly, while network dynamics can mimic the target dynamics. This demonstrates that the holistic setup of the network model and the neuron model which are both more biologically plausible than previous work, can be tuned into real biological signal dynamics.
Analysis of fecal bile acids and metabolites by high resolution mass spectrometry in farm animals and correlation with microbiota
Authors: Porru Emanuele , Scicchitano Daniel , Interino Nicolò , Tavella Teresa , Candela Marco , Roda Aldo , Fiori Jessica
Published: February 2022

There is a growing interest in the named “acidic sterolbiome” and in the genetic potential of the gut microbiome (GM) to modify bile acid (BA) structure. Indeed, the qualitative composition of BAs in feces correlates with the bowel microorganisms and their collective genetic material. GM is responsible for the production of BA metabolites, such as secondary and oxo-BAs. The specific BA profiles, as microbiome-host co-metabolic products, could be useful to investigate the GM-host interaction in animals under physiological conditions, as well as in specific diseases. In this context, we developed and validated an ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry method for the simultaneous analysis of up to 21 oxo-BAs and their 9 metabolic precursors. Chromatographic separation was achieved in 7 min with adequate analytical performance in terms of selectivity, sensitivity (LOQ from 0.05 to 0.1 µg/mL), accuracy (bias% < 5%), precision (CV% < 5%) and matrix effect (ME% < 10%). A fast solvent extraction protocol has been fine-tuned, achieving recoveries > 90%. In parallel, the gut microbiota assessment in farming animals was evaluated by 16S rRNA next-generation sequencing, and the correlation with the BA composition was performed by multivariate analysis, allowing to reconstruct species-specific associations between the BA profile and specific GM components.
Association between recurrent breast cancer and phthalate exposure modified by hormone receptors and body mass index
Authors: Yang Pei-Jing , Hou Ming-Feng , Ou-Yang Fu , Hsieh Tsung-Hua , Lee Yen-Jung , Tsai Eing-Mei , Wang Tsu-Nai
Published: February 2022

The association between phthalate exposure and breast cancer remains controversial. We performed a prospective patient cohort design to explore the interaction between creatinine-corrected urinary phthalate metabolites and hormone receptors as well as body mass index (BMI) on recurrent breast cancer. In this follow-up study, 636 female breast cancer patients and 45 new recurrent cases diagnosed for a total of 1576.68 person-years of follow-up were recruited. Mono-(2-ethyl-5-oxohexyl) phthalate (MEOHP) was negatively associated with breast cancer recurrence, with adjusted hazard ratio (aHR) 3rd vs. 1st quartile of 0.15 (95% CI 0.04–0.51). The MEOHP presented as a non-monotonic dose–response (NMDR) curve, being U-shaped. In the stratification of hormone receptors, MEOHP still exhibited a U-shaped dose–response curve. The third quartile of MEOHP showed significant lowest recurrent risk in the status of ER-positive (aHR 0.18, 95% CI 0.05–0.66), PR-negative (aHR 0.14, 95% CI 0.03–0.63), and HER2-negative (aHR 0.24, 95% CI 0.08–0.76). Whether in BMI < 25 or in BMI ≥ 25, the third quartile of MEOHP was negatively associated with recurrent breast cancer, and there was a negative interaction on an additive scale between MEOHP and BMI (pinteraction = 0.042). The association between MEOHP and recurrent breast cancer was modified by hormone receptors and BMI.
Automated human cell classification in sparse datasets using few-shot learning
Authors: Walsh Reece , Abdelpakey Mohamed H. , Shehata Mohamed S. , Mohamed Mostafa M.
Published: February 2022

Classifying and analyzing human cells is a lengthy procedure, often involving a trained professional. In an attempt to expedite this process, an active area of research involves automating cell classification through use of deep learning-based techniques. In practice, a large amount of data is required to accurately train these deep learning models. However, due to the sparse human cell datasets currently available, the performance of these models is typically low. This study investigates the feasibility of using few-shot learning-based techniques to mitigate the data requirements for accurate training. The study is comprised of three parts: First, current state-of-the-art few-shot learning techniques are evaluated on human cell classification. The selected techniques are trained on a non-medical dataset and then tested on two out-of-domain, human cell datasets. The results indicate that, overall, the test accuracy of state-of-the-art techniques decreased by at least 30% when transitioning from a non-medical dataset to a medical dataset. Reptile and EPNet were the top performing techniques tested on the BCCD dataset and HEp-2 dataset respectively. Second, this study evaluates the potential benefits, if any, to varying the backbone architecture and training schemes in current state-of-the-art few-shot learning techniques when used in human cell classification. To this end, the best technique identified in the first part of this study, EPNet, is used for experimentation. In particular, the study used 6 different network backbones, 5 data augmentation methodologies, and 2 model training schemes. Even with these additions, the overall test accuracy of EPNet decreased from 88.66% on non-medical datasets to 44.13% at best on the medical datasets. Third, this study presents future directions for using few-shot learning in human cell classification. In general, few-shot learning in its current state performs poorly on human cell classification. The study proves that attempts to modify existing network architectures are not effective and concludes that future research effort should be focused on improving robustness towards out-of-domain testing using optimization-based or self-supervised few-shot learning techniques.
Design, synthesis and biological activities of echinopsine derivatives containing acylhydrazone moiety
Authors: Cui Peipei , Cai Mingjiang , Meng Yanan , Yang Yan , Song Hongjian , Liu Yuxiu , Wang Qingmin
Published: February 2022

Based on the broad-spectrum biological activities of echinopsine and acylhydrazones, a series of echinopsine derivatives containing acylhydrazone moieties have been designed, synthesized and their biological activities were evaluated for the first time. The bioassay results indicated that most of the compounds showed moderate to good antiviral activities against tobacco mosaic virus (TMV), among which echinopsine (I) (inactivation activity, 49.5 ± 4.4%; curative activity, 46.1 ± 1.5%; protection activity, 42.6 ± 2.3%) and its derivatives 1 (inactivation activity, 44.9 ± 4.6%; curative activity, 39.8 ± 2.6%; protection activity, 47.3 ± 4.3%), 3 (inactivation activity, 47.9 ± 0.9%; curative activity, 43.7 ± 3.1%; protection activity, 44.6 ± 3.3%), 7 (inactivation activity, 46.2 ± 1.6%; curative activity, 45.0 ± 3.7%; protection activity, 41.7 ± 0.9%) showed higher anti-TMV activity in vivo at 500 mg/L than commercial ribavirin (inactivation activity, 38.9 ± 1.4%; curative activity, 39.2 ± 1.8%; protection activity, 36.4 ± 3.4%). Some compounds exhibited insecticidal activities against Plutella xylostella, Mythimna separate and Spodoptera frugiperda. Especially, compounds 7 and 27 displayed excellent insecticidal activities against Plutella xylostell (mortality 67 ± 6% and 53 ± 6%) even at 0.1 mg/L. Additionally, most echinopsine derivatives exhibited high fungicidal activities against Physalospora piricola and Sclerotinia sclerotiorum.
Polarisation-sensitive optical coherence tomography measurement of retardance in fibrosis, a non-invasive biomarker in patients with systemic sclerosis
Authors: Marjanovic E. J. , Sharma V. , Smith L. , Pinder C. , Moore T. L. , Manning J. B. , Dinsdale G. , Berks M. , Newton V. L. , Wilkinson S. , Dickinson M. R. , Herrick A. L. , Watson R. E. B. , Murray A. K.
Published: February 2022

Polarisation-sensitive optical coherence tomography (PS-OCT) offers a novel, non-invasive method of assessing skin fibrosis in the multisystem disease systemic sclerosis (SSc) by measuring collagen retardance. This study aimed to assess retardance as a biomarker in SSc. Thirty-one patients with SSc and 27 healthy controls (HC) underwent PS-OCT imaging. ‘Skin score’ was assessed by clinical palpation (0–3 scale). A subset of ten patients and ten age/sex-matched HC had a biopsy and longitudinal imaging. Histological assessment included quantification of epidermal thickness, collagen content (to assess fibrosis) and matrix metalloproteinase (MMP) activity (in situ zymography). PS-OCT images were assessed for epidermal thickness (structure) and fibrosis (retardance). Positive correlation was observed between epidermal thickness as measured by histology and structural PS-OCT (r = 0.79; p < 0.001). Retardance was: HC mean 0.21 (SD 0.21) radian/pixel; SSc skin score 0, 0.30 (0.19); skin score 1, 0.11 (0.16); skin score 2, 0.06 (0.12); skin score 3, 0.36 (0.35). Longitudinal retardance decreased at one-week across groups, increasing at one-month for HC/skin score 0–1; HC biopsy site retardance suggests scarring is akin to fibrosis. Relationships identified between retardance with both biopsy and skin score data indicate that retardance warrants further investigation as a suitable biomarker for SSc-related fibrosis.
A tropical stratopause precursor for sudden stratospheric warmings
Authors: Koushik N. , Kumar K. Kishore , Pramitha M.
Published: February 2022

Dramatic meteorological phenomena in the winter polar stratosphere known as Sudden Stratospheric Warming (SSW) events are well recognized for their impacts felt across the whole atmosphere. Apart from the influence of tropospheric forcing and stratospheric control, many studies have addressed the possible role of external factors on the occurrence of SSW events. Here, with the help of reanalysis datasets, we present a hitherto unexplored connection between the tropical upper stratosphere and the polar vortex. We identify enhanced planetary wave driving around the tropical stratopause and poleward progression of the zero-wind line as early indicators for the occurrence of SSW events. We demonstrate that the poleward progression of the zero wind line results in efficient focusing of planetary waves into the polar vortex which culminates in its disruption. Statistically, nearly 70% of the SSW events that took place so far have been preceded by enhanced tropical stratopause wave driving which points towards identifying this as a potential precursor for the occurrence of SSW events. After the year 2000, significantly a greater number of SSW events have been found to be preceded by enhanced tropical stratopause wave driving.
Effects of symmetry breaking of the structurally-disordered Hamiltonian ensembles on the anisotropic decoherence of qubits
Authors: Chen Hong-Bin
Published: February 2022

It is commonly known that the dephasing in open quantum systems is due to the establishment of bipartite correlations with ambient environments, which are typically difficult to be fully characterized. Recently, a new approach of average over disordered Hamiltonian ensemble is developed and shown to be capable of describing the nonclassicality of incoherent dynamics based on inferring the nonclassical nature of the correlations. Here we further extend the approach of Hamiltonian ensemble in the canonical form to the realm of structural disorder. Under the variable separation of the probability distribution within the Hamiltonian ensemble, the geometrical structure is easily visualized and can be characterized according to the degree of symmetry. We demonstrate four degrees and investigate the effects of different types of symmetry breaking on the incoherent dynamics. We show that these effects are easily understood from the emergences of additional terms in the master equations, leading to rather general master equations and, consequently, going beyond the previous frameworks of pure dephasing or isotropic depolarization.
LPS-induced lipid alterations in microglia revealed by MALDI mass spectrometry-based cell fingerprinting in neuroinflammation studies
Authors: Blank Martina , Enzlein Thomas , Hopf Carsten
Published: February 2022

Pathological microglia activation can promote neuroinflammation in many neurodegenerative diseases, and it has therefore emerged as a potential therapeutic target. Increasing evidence suggests alterations in lipid metabolism as modulators and indicators in microglia activation and its effector functions. Yet, how lipid dynamics in activated microglia is affected by inflammatory stimuli demands additional investigation to allow development of more effective therapies. Here, we report an extensive matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS) whole cell fingerprinting workflow to investigate inflammation-associated lipid patterns in SIM-A9 microglial cells. By combining a platform of three synergistic MALDI MS technologies we could detect substantial differences in lipid profiles of lipopolysaccharide (LPS)- stimulated and unstimulated microglia-like cells leading to the identification of 21 potential inflammation-associated lipid markers. LPS-induced lipids in SIM-A9 microglial cells include phosphatidylcholines, lysophosphatidylcholines (LysoPC), sphingolipids, diacylglycerols and triacylglycerols. Moreover, MALDI MS-based cell lipid fingerprinting of LPS-stimulated SIM-A9 microglial cells pre-treated with the non-selective histone deacetylase inhibitor suberoylanilide hydroxamic acid revealed specific modulation of LPS-induced-glycerolipids and LysoPC(18:0) with a significant reduction of microglial inflammation response. Our study introduces MALDI MS as a complementary technology for fast and label-free investigation of stimulus-dependent changes in lipid patterns and their modulation by pharmaceutical agents.
Longitudinal analysis of 5-year refractive changes in a large Japanese population
Authors: Takeuchi Masaki , Meguro Akira , Yoshida Masao , Yamane Takahiro , Yatsu Keisuke , Okada Eiichi , Mizuki Nobuhisa
Published: February 2022

Refractive changes are reportedly affected by age, sex, and current refractive error. To clarify the pattern of refractive changes in a Japanese population, we conducted a 5-year follow-up longitudinal analysis of spherical equivalent (SE) refractive changes with stratification by sex, age, and SE in 593,273 eyes from Japanese individuals ages 3–91 years. The 5-year SE change with myopic shift dramatically increased over time after age 4 years, and the largest change was observed in both males and females who were age 8 years at baseline [males: − 2.654 ± 0.048 diopters (D); females: − 3.110 ± 0.038 D]. During school age, the 5-year myopic change was greater in females than in males, and emmetropic and low-to-moderate myopic eyes underwent larger myopic changes than hyperopic and high-to-severe myopic eyes. After the peak at age 8 years, the 5-year myopic change gradually declined with age and fell below − 0.25 D at age 27 in males and age 26 years in females. The 5-year SE changes transitioned from a myopic to a hyperopic shift at age 51 in both sexes, and hyperopization advanced more quickly in hyperopic eyes. Our findings highlight the importance of myopia prevention in school-aged children.