bluuebunny/crossref_metadata_2025 huggingface dataset:
A Noble Extract of Pseudomonas sp. M20A4R8 Efficiently Controlling the Influenza Virus-Induced Cell Death
Authors: Jung Su-Bin , Choi Grace , Kim Hyo-Jin , Moon Kyeong-Seo , Lee Gun , Na Kyeong-Hak , Kwon Yong Min , Moon Jimin , Shin Mi Yeong , Yu Jae-Yeong , Baek Yeong-Bin , Park Jun-Gyu , Park Sang-Ik
Published: March 2024

Epidemic diseases that arise from infectious RNA viruses, particularly influenza viruses, pose a constant threat to the global economy and public health. Viral evolution has undermined the efficacy of acquired immunity from vaccines and the antiviral effects of FDA-approved drugs. As such, there is an urgent need to develop new antiviral lead agents. Natural compounds, owing to their historical validation of application and safety, have become a promising solution. In this light, a novel marine bacterium, Pseudomonas sp. M20A4R8, has been found to exhibit significant antiviral activity [half maximal inhibitory concentration (IC50) = 1.3 µg/mL, selectivity index (SI) = 919.4] against influenza virus A/Puerto Rico/8/34, surpassing the activity of chloroquine. The antiviral response via M20A4R8 extract was induced during post-entry stages of the influenza virus, indicating suitability for post-application after the establishment of viral infection. Furthermore, post-treatment with M20A4R8 extract protected the host from virus-induced apoptosis, suggesting its potential use in acute respiratory disease complexes resulting from immune effectors’ overstimulation and autophagy-mediated self-apoptosis. The extract demonstrated an outstanding therapeutic index against influenza virus A/Wisconsin/15/2009 (IC50 = 8.1 µg/mL, SI = 146.2) and B/Florida/78/2015 Victoria lineage (IC50 = 3.5 µg/mL, SI = 343.8), indicating a broad anti-influenza virus activity with guaranteed safety and effectiveness. This study provides a new perspective on mechanisms for preventing a broad spectrum of viral infections through antiviral agents from novel and natural origins. Future studies on a single or combined compound from the extract hold promise, encouraging its use in preclinical challenge tests with various influenza virus strains.
Comparison of Copper-Tolerance Genes between Different Groups of Acidovorax citrulli
Authors: Zhang Min , Zhao Mei , Qiao Pei , Liu Dehua , Bai Qingrong , Guan Wei , Yang Yuwen , Zhao Tingchang
Published: March 2024

Acidovorax citrulli populations exhibit genetic and phenotypic variations, particularly in terms of copper tolerance. Group I strains of A. citrulli generally exhibit higher copper tolerance compared to group II strains. This study aims to identify genes involved in copper tolerance to better understand the differences in copper tolerance between group I and group II strains. Representative strains pslb65 (group I) and pslbtw14 (group II) were selected for comparison. Deletion mutants of putative copper-tolerance genes and their corresponding complementary strains were constructed. The copper tolerance of each strain was evaluated using the minimum inhibitory concentration method. The results showed that the copA, copZ, cueR, and cueO genes played major roles in copper tolerance in A. citrulli, while cusC-like, cusA-like, and cusB-like genes had minor effects. The different expression levels of copper-tolerance-related genes in pslb65 and pslbtw14 under copper stress indicated that they had different mechanisms for coping with copper stress. Overall, this study provides insights into the mechanisms of copper tolerance in A. citrulli and highlights the importance of specific genes in copper tolerance.
Occurrence of State of Gold in Crude Oil and Its Economic Significance
Authors: Ni Zhiyong , Zhang Wen , Liu Jie , Shi Shengbao , Wang Xue , Su Yang
Published: March 2024

Gold and petroleum are also strategic resources of great importance to national security. With the increasing demand for energy, multi-energy cooperative exploration has become an inevitable trend of resource development and utilization. Petroleum and hydrothermal gold deposits may form together, with similar evolutionary trends in their formation, migration, and enrichment. Petroleum reservoirs and gold deposits are closely coupled under certain geological conditions. The solubility of gold in crude oil and its forms of occurrence are important in determining the mechanisms of interaction between gold and petroleum and in facilitating the recovery of gold from gold-bearing petroleum. In this study, the occurrence of gold in crude oil from the Linnan Depression in the Bohai Bay Basin, China, was studied using inductively coupled plasma–mass spectrometry and X-ray photoelectron spectroscopy. Concentrations of gold in crude oil from the Linpan and Shanghe oilfields averaged 44.5 ppb, which is well above the minimum concentration required for hydrothermal gold mineralization. Gold has an affinity with carbon, oxygen, and sulfur, and its concentration in crude oil is positively correlated with total acid and sulfur contents. We speculate that gold may exist in crude oil as complexes with organic acids or thiols, with crude oil thus being a transport medium for gold.
Global Occurrence, Geology and Characteristics of Hydrothermal-Origin Kaolin Deposits
Authors: Ece Ömer Işık , Ercan Hatice Ünal
Published: March 2024

Kaolin-group minerals occur in nature as the result of high-sulfidation acid sulfate, sulfur-poor HCl-, HF- and H2CO3-rich acidic fluid-related hydrothermal alterations and in situ geochemical weathering. These minerals possess different crystallographic and chemical properties that determine their application areas, mainly in the ceramic and paper industries, and as nanocomposite materials. The physicochemical properties of hydrothermal kaolin deposits are the result of the type of parent rock, the effect of the regional tectonism-associated magmatism, and the chemical features of hydrothermal fluids that interact with the deep basement rocks. However, understanding these geothermal systems is one of the most challenging issues due to the rich mineralogical assemblages, complex geochemistry and isotopic data of hydrothermal alteration zones. This study evaluates the formation of hydrothermal-origin kaolin-group minerals by considering their characteristics of hydrothermal alteration, isotopic compositions and differences in characteristic properties of low- and high-sulfidation occurrences; this paper also addresses mineralogical and structural differences between hypogene and supergene kaolin formations, and kaolin–alunite–pyrophyllite association, and it provides examples of worldwide occurrences. The study of the mineralogical assemblages, geochemistry and isotopic data of the hydrothermal alteration zones is one of the most challenging subjects in terms of gaining a detailed understanding of the geothermal systems. Silicification processes are subsequent to late-stage alteration after the completion of kaolinization processes, erasing existing hydrothermal mineralogical and geochemical traces and making interpretation difficult. In the early stages involving magmatic–hydrothermal-origin acidic geothermal fluids, the latter comes from the disproportionation of SO2 (+H2O) and H2S oxidation to H2SO4 in hydrothermal environments. In the later stages, due to spatial and temporal changes over time in the chemistry of geothermal fluids, the system comes to have a more alkali–chloride composition, with neutral pH waters frequently saturated with amorphous silica which characteristically precipitate as siliceous sinter deposits containing large amounts of opal-A.
Machine-Learning Analysis of the Canadian Royalties Grinding Circuit
Authors: Di Feo Antonio , Khodaie Nasseh , Girard Matthieu , Michaud Simon
Published: March 2024

This work aimed to understand the relationships between grinding variables and the P80 (80% passing size) of a grinding circuit (feed to flotation). Canadian Royalties want to obtain and reduce variations in the P80, which is currently 65 micrometres. Thus, principal component analysis (PCA), part of machine learning, was utilized to better understand the factors that significantly influence the P80. PCA is meant to be used as a guideline for plant metallurgists to determine how the grinding circuit factors influence P80; thus, the variables can be manipulated to lower P80 fluctuations. PCA revealed that the head grade of the ore (pentlandite (Pn), chalcopyrite (Cp), pyrrhotite (Po) and non-sulphide gangue (NSG)) and the primary ball mill power were weakly correlated with P80. However, the ore level in the silo, flowrate to cyclones, cyclone pressure, percent solids and the feed tonnage rate to the primary ball mill were strongly correlated with P80. This information can be used to develop a strategy to control the P80 of the grinding circuit and provide a more consistent grind size to the flotation circuit, which can positively impact metallurgical performance.
The Monitoring and Cell Imaging of Fe3+ Using a Chromone-Based Fluorescence Probe
Authors: Bian Yongjun , Qu Xingyu , Zhang Fengying , Zhang Zhengwei , Kang Jin
Published: March 2024

A new structurally simple fluorescent CP probe based on chromone was designed and synthesized, and its structure was fully characterized using various analytical techniques. The CP probe displays a high selectivity and sensitivity for sensing Fe3+ with a “turn-off” fluorescence response over other metal ions in a DMSO/H2O (4:1, v/v) solution. The experiment results show that the CP probe is stable over a wide pH range of 2.0–12.0. The detection limit for Fe3+ was calculated to be 0.044 μmol•L−1. The molar ratio method indicated that the binding mode between the CP probe and Fe3+ is a 1:1 complex formation. HR-MS and density functional theory (DFT) calculations were also performed to further confirm the recognition mechanism. Both fluorescence imaging experiments and the MTT assay demonstrated that the CP probe was suitable for detecting intracellular Fe3+ and no significant cytotoxicity in living cells.
Exploring Geometrical, Electronic and Spectroscopic Properties of 2-Nitroimidazole-Based Radiopharmaceuticals via Computational Chemistry Methods
Authors: Crișan George , Stan Ștefan , Chiș Vasile
Published: March 2024

Tumor hypoxia plays an important role in the clinical management and treatment planning of various cancers. The use of 2-nitroimidazole-based radiopharmaceuticals has been the most successful for positron emission tomography (PET) and single photon emission computed tomography (SPECT) imaging probes, offering noninvasive means to assess tumor hypoxia. In this study we performed detailed computational investigations of the most used compounds for PET imaging, focusing on those derived from 2-nitroimidazole: fluoromisonidazole (FMISO), fluoroazomycin arabinoside (FAZA), fluoroetanidazole (FETA), fluoroerythronitroimidazole (FETNIM) and 2-(2-nitroimidazol-1-yl)-N-(2,2,3,3,3-pentafluoropropyl)acetamide (EF5). Conformational analysis, structural parameters, vibrational IR and Raman properties (within both harmonic and anharmonic approximations), as well as the NMR shielding tensors and spin-spin coupling constants were obtained by density functional theory (DFT) calculations and then correlated with experimental findings, where available. Furthermore, time-dependent DFT computations reveal insight into the excited states of the compounds. Our results predict a significant change in the conformational landscape of most of the investigated compounds when transitioning from the gas phase to aqueous solution. According to computational data, the 2-nitroimidazole moiety determines to a large extent the spectroscopic properties of its derivatives. Due to the limited structural information available in the current literature for the investigated compounds, the findings presented herein deepen the current understanding of the electronic structures of these five radiopharmaceuticals.
Enhanced Stability of Dimethyl Ether Carbonylation through Pyrazole Tartrate on Tartaric Acid-Complexed Cobalt–Iron-Modified Hydrogen-Type Mordenite
Authors: Fu Guangtao , Dong Xinfa
Published: March 2024

In this study, pyrazole tartrate (Pya·DL) and tartaric acid (DL) complexed with cobalt–iron bimetallic modified hydrogen-type mordenite (HMOR) were prepared using the ion exchange method. The results demonstrate that the stability of the dimethyl ether (DME) carbonylation reaction to methyl acetate (MA) was significantly improved after the introduction of Pya·DL to HMOR. The Co∙Fe∙DL-Pya·DL-HMOR (0.8) sample exhibited sustainable stability within 400 h DME carbonylation, exhibiting a DME conversion rate of about 70% and MA selectivity of above 99%. Through modification with the DL-complexed cobalt–iron bimetal, the dispersion of cobalt–iron was greatly enhanced, leading to the formation of new metal Lewis acidic sites (LAS) and thus a significant improvement in catalysis activity. Pya·DL effectively eliminated non-framework aluminum in HMOR, enlarged its pore size, and created channels for carbon deposition diffusion, thereby preventing carbon accumulation and pore blockage. Additionally, Pya·DL shielded the Bronsted acid sites (BAS) in the 12 MR channel, effectively suppressing the side reactions of carbon deposition and reducing the formation of hard carbon deposits. These improvements collectively contribute to the enhanced stability of the DME carbonylation reaction.
Construction of a Visible-Light-Response Photocatalysis–Self-Fenton Degradation System of Coupling Industrial Waste Red Mud to Resorcinol–Formaldehyde Resin
Authors: Lv Xiangxiu , Yuan Hao , Sun Kaiqu , Shi Weilong , Li Chunsheng , Guo Feng
Published: March 2024

Heterogeneous photocatalysis–self-Fenton technology is a sustainable strategy for treating organic pollutants in actual water bodies with high-fluent degradation and high mineralization capacity, overcoming the limitations of the safety risks caused by adding external iron sources and hazardous chemicals in the homogeneous Fenton reaction and injecting high-intensity energy fields in photo-Fenton reaction. Herein, a photo-self-Fenton system based on resorcinol–formaldehyde (RF) resin and red mud (RM) was established to generate hydrogen peroxide (H2O2) in situ and transform into hydroxy radical (•OH) for efficient degradation of tetracycline (TC) under visible light irradiation. The capturing experiments and electron spin resonance (ESR) confirmed that the hinge for the enhanced performance of this system is the superior H2O2 yield (499 μM) through the oxygen reduction process (ORR) of the two-step single-electron over the resin and the high concentration of •OH due to activation effect of RM. In addition, the Fe2+/Fe3+ cycles are accelerated by photoelectrons to effectively initiate the photo-self-Fenton reaction. Finally, the possible degradation pathways were proposed via liquid chromatography-mass spectrometry (LC-MS). This study provides a new idea for environmental recovery in a waste-based heterogeneous photocatalytic self-Fenton system.
Exploring the Chemical Profile, In Vitro Antioxidant and Anti-Inflammatory Activities of Santolina rosmarinifolia Extracts
Authors: Schmidt Janos , Juhasz Kata , Bona Agnes
Published: March 2024

In this study, the phytochemical composition, in vitro antioxidant, and anti-inflammatory effects of the aqueous and 60% ethanolic (EtOH) extracts of Santolina rosmarinifolia leaf, flower, and root were examined. The antioxidant activity of S. rosmarinifolia extracts was determined by 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assays. The total phenolic content (TPC) of the extracts was measured by the Folin–Ciocalteu assay. The anti-inflammatory effect of the extracts was monitored by the Griess assay. The chemical composition of S. rosmarinifolia extracts was analysed using the LC-MS technique. According to our findings, 60% EtOH leaf extracts showed the highest Trolox equivalent antioxidant capacity (TEAC) values in both ABTS (8.39 ± 0.43 µM) and DPPH (6.71 ± 0.03 µM) antioxidant activity assays. The TPC values of the samples were in good correspondence with the antioxidant activity measurements and showed the highest gallic acid equivalent value (130.17 ± 0.01 µg/mL) in 60% EtOH leaf extracts. In addition, the 60% EtOH extracts of the leaves were revealed to possess the highest anti-inflammatory effect. The LC-MS analysis of S. rosmarinifolia extracts proved the presence of ascorbic acid, catalpol, chrysin, epigallocatechin, geraniol, isoquercitrin, and theanine, among others, for the first time. However, additional studies are needed to investigate the direct relationship between the chemical composition and physiological effects of the herb. The 60% EtOH extracts of S. rosmarinifolia leaves are potential new sources of natural antioxidants and anti-inflammatory molecules in the production of novel nutraceutical products.
Identification of Vernonia patula Merr. and Its Similar Varieties Based on a Combination of HPLC Fingerprinting and Chemical Pattern Recognition
Authors: Liu Wen , Huang Liyuan , Zhu Jiashan , Lu Liwen , Su Xiaoling , Hou Xiaotao , Xiao Zeen
Published: March 2024

Vernonia patula Merr. (VP) is a traditional medicine used by the Zhuang and Yao people, known for its therapeutic properties in treating anemopyretic cold and other diseases. Distinguishing VP from similar varieties such as Praxelis clematidea (PC), Ageratum conyzoides L. (AC) and Ageratum houstonianum Mill (AH) was challenging due to their similar traits and plant morphology. The HPLC fingerprints of 40 batches of VP and three similar varieties were established. SPSS 20.0 and SIMCA-P 13.0 were used to statistically analyze the chromatographic peak areas of 37 components. The results showed that the similarity of the HPLC fingerprints for each of the four varieties was >0.9, while the similarity between the control chromatogram of VP and its similar varieties was <0.678. Cluster analysis and partial least squares discriminant analysis provided consistent results, indicating that all four varieties could be individually clustered together. Through further analysis, we found isochlorogenic acid A and isochlorogenic acid C were present only in the original VP, while preconene II was present in the three similar varieties of VP. These three components are expected to be identification points for accurately distinguishing VP from PC, AC and AH.
4-Chloroisocoumarins as Chlamydial Protease Inhibitors and Anti-Chlamydial Agents
Authors: Phillips Matthew J. A. , Huston Wilhelmina M. , McDonagh Andrew M. , Rawling Tristan
Published: March 2024

4-Chloroisocoumarin compounds have broad inhibitory properties against serine proteases. Here, we show that selected 3-alkoxy-4-chloroisocoumarins preferentially inhibit the activity of the conserved serine protease High-temperature requirement A of Chlamydia trachomatis. The synthesis of a new series of isocoumarin-based scaffolds has been developed, and their anti-chlamydial properties were investigated. The structure of the alkoxy substituent was found to influence the potency of the compounds against High-temperature requirement A, and modifications to the C-7 position of the 3-alkoxy-4-chloroisocoumarin structure attenuate anti-chlamydial properties.
Identification of Corn Peptides with Alcohol Dehydrogenase Activating Activity Absorbed by Caco-2 Cell Monolayers
Authors: Wang Zhe , Li Guanlong , Liu Xiaolan
Published: March 2024

Alcohol dehydrogenase (ADH) plays a pivotal role in constraining alcohol metabolism. Assessing the ADH-activating activity in vitro can provide insight into the capacity to accelerate ethanol metabolism in vivo. In this study, ADH-activating peptides were prepared from corn protein meal (CGM) using enzymatic hydrolysis, and these peptides were subsequently identified following simulated gastrointestinal digestion and their absorption through the Caco-2 cell monolayer membrane. The current investigation revealed that corn protein hydrolysate hydrolyzed using alcalase exhibited the highest ADH activation capability, maintaining an ADH activation rate of 52.93 ± 2.07% following simulated gastrointestinal digestion in vitro. After absorption through the Caco-2 cell monolayer membrane, ADH-activating peptides were identified. Among them, SSNCQPF, TGCPVLQ, and QPQQPW were validated to possess strong ADH activation activity, with EC50 values of 1.35 ± 0.22 mM, 2.26 ± 0.16 mM, and 2.73 ± 0.13 mM, respectively. Molecular Docking revealed that the activation of ADH occurred via the formation of a stable complex between the peptide and the active center of ADH by hydrogen bonds and hydrophobic interactions. The results of this study also suggest that corn protein hydrolysate could be a novel functional dietary element that helps protects the liver from damage caused by alcohol and aids in alcohol metabolism.
The Roles of Impurities and Surface Area on Thermal Stability and Oxidation Resistance of BN Nanoplatelets
Authors: Kostoglou Nikolaos , Stock Sebastian , Solomi Angelos , Holzapfel Damian M. , Hinder Steven , Baker Mark , Constantinides Georgios , Ryzhkov Vladislav , Maletaskic Jelena , Matovic Branko , Schneider Jochen M. , Rebholz Claus , Mitterer Christian
Published: March 2024

This study considers the influence of purity and surface area on the thermal and oxidation properties of hexagonal boron nitride (h-BN) nanoplatelets, which represent crucial factors in high-temperature oxidizing environments. Three h-BN nanoplatelet-based materials, synthesized with different purity levels and surface areas (~3, ~56, and ~140 m2/g), were compared, including a commercial BN reference. All materials were systematically analyzed by various characterization techniques, including gas pycnometry, scanning electron microscopy, X-ray diffraction, Fourier-transform infrared radiation, X-ray photoelectron spectroscopy, gas sorption analysis, and thermal gravimetric analysis coupled with differential scanning calorimetry. Results indicated that the thermal stability and oxidation resistance of the synthesized materials were improved by up to ~13.5% (or by 120 °C) with an increase in purity. Furthermore, the reference material with its high purity and low surface area (~4 m2/g) showed superior performance, which was attributed to the minimized reactive sites for oxygen diffusion due to lower surface area availability and fewer possible defects, highlighting the critical roles of both sample purity and accessible surface area in h-BN thermo-oxidative stability. These findings highlight the importance of focusing on purity and surface area control in developing BN-based nanomaterials, offering a path to enhance their performance in extreme thermal and oxidative conditions.
Soy Protein Concentrate Diets Inversely Affect LPS-Binding Protein Expression in Colon and Liver, Reduce Liver Inflammation, and Increase Fecal LPS Excretion in Obese Zucker Rats
Authors: Li Wei , Hakkak Reza
Published: March 2024

Dietary soy protein and soy isoflavones have anti-inflammatory properties. Previously, we reported that feeding soy protein concentrate diet (SPC) with low or high isoflavone (LIF or HIF) to young (seven-week-old) obese (fa/fa) Zucker rats inhibits lipopolysaccharide (LPS) translocation and decreases liver inflammation compared to a casein control (CAS) diet. The current study investigated whether SPC-LIF and SPC-HIF diets would reduce liver inflammation in adult obese Zucker rats fed a CAS diet. A total of 21 six-week-old male obese (fa/fa) Zucker rats were given CAS diet for 8 weeks to develop obesity then randomly assigned to CAS, SPC-LIF, or SPC-HIF (seven rats/group) diet for an additional 10 weeks. The expression of LPS-translocation, inflammation, and intestinal permeability markers were quantified by qPCR in liver, visceral adipose tissue (VAT), and colon. LPS concentration was determined in both the colon content and fecal samples by a Limulus amebocyte lysate (LAL) test. SPC-LIF and SPC-HIF diets significantly decreased liver LPS-binding protein (LBP) expression compared to CAS diet (p < 0.01 and p < 0.05, respectively). SPC-HIF diet also significantly decreased liver MCP-1 and TNF-α expression (p < 0.05) and had a trend to decrease liver iNOS expression (p = 0.06). In the colon, SPC-HIF diet significantly increased LBP expression compared to CAS diet (p < 0.05). When samples from all three groups were combined, there was a negative correlation between colon LBP expression and liver LBP expression (p = 0.046). SPC diets did not alter the expression of intestinal permeability markers (i.e., occludin, claudin 3, and zonula occludens-1) in the colon or inflammation markers (i.e., TNF-α and iNOS) in VAT or the colon. LPS levels in the colon content did not differ between any groups. Fecal LPS levels were significantly higher in the SPC-LIF and SPC-HIF groups compared to the CAS group (p < 0.01). In conclusion, SPC, particularly SPC with HIF, reduces liver LBP expression and inflammation makers (i.e., TNF-α and MCP-1 expression) in adult obese Zucker rats, likely by reducing LPS translocation.
Unlocking Cognitive Potential: Association of Sarcopenia and Mediterranean Diet on Cognitive Function in Community-Dwelling Elderly of the Dalmatian Region
Authors: Jelaska Julija , Vučković Marijana , Gugić Ordulj Ivana , Kolak Ela , Šolić Šegvić Lucija , Đapić Kolak Zdravka , Keser Irena , Radić Josipa
Published: March 2024

The aim of this study was to determine the association between muscle strength, adherence to the Mediterranean diet (MeDi) and cognitive function in community-dwelling elderly. General data, data of body composition and anthropometric parameters, clinical and laboratory findings, cognitive test questionnaires (Mini-Mental State Examination—MMSE, Trail Making Test—TMT, Symbol Digit Modalities Test—SDMT), and nutritional assessments (Mini Nutritional Assessment—MNA, Mediterranean Diet Serving Score—MDSS) were obtained for each study participant. Handgrip strength (HS) was used as one of the key parameters for defining probable sarcopenia, among the Short Physical Performance Battery test (SPPB) (for defining physical activity) and the strength, assistance with walking, rising from a chair, climbing stairs, and falls questionnaire (SARC-F). Our cross-sectional study involved 114 participants aged ≥ 60 years, and two-thirds of the participants were female (76.3% vs. 23.7%). Probable sarcopenia was found in 34.7% of them. Using bivariate regression analysis, cognitive deficit among the sarcopenic population was associated with the following groups of collected data: (a) sociodemographic-associated factors—advanced age (OR: 1.07; p = 0.004), single marital status (OR: 3.25; p = 0.03), and low level of education (OR: 0.22; p < 0.003); (b) behavioral-associated factors—duration of institutionalization (OR: 1.05; p = 0.007), performance of heavy physical work (OR: 6.26; p = 0.001), low physical activity (OR: 0.08; p = 0.002), and risk of malnutrition (OR: 3.87; p = 0.005); (c) disease-related factors—loss of appetite (OR: 2.24; p = 0.04), information processing speed (OR: 0.88; p < 0.001), blood pressure systolic/diastolic variables (OR: 0.96/0.96; p = 0.002/0.02), medications (OR: 1.19; p = 0.005), predictive sarcopenia score ≥ 4 (OR: 3.1; p = 0.003), and low muscle strength (OR: 0.92; p = 0.002). Cognitive preservation among the sarcopenic population was associated with married status (OR: 0.23; p = 0.20), a high level of education (OR: 0.18; p = 0.002), smoking (OR: 0.33; p = 0.02), high physical activity (OR: 0.07; p < 0.001), and dietary habits using poultry (OR: 0.12; p = 0.004). The results suggest a significant association between sarcopenia and cognitive function in community-dwelling elderly, highlighting the need for regular nutritional interventions in this special population.
Dysregulated Glucuronidation of Bilirubin Exacerbates Liver Inflammation and Fibrosis in Schistosomiasis Japonica through the NF-κB Signaling Pathway
Authors: Xue Qingkai , Wang Yuyan , Liu Yiyun , Hua Haiyong , Zhou Xiangyu , Xu Yongliang , Zhang Ying , Xiong Chunrong , Liu Xinjian , Yang Kun , Huang Yuzheng
Published: March 2024

Hepatic fibrosis is an important pathological manifestation of chronic schistosome infection. Patients with advanced schistosomiasis show varying degrees of abnormalities in liver fibrosis indicators and bilirubin metabolism. However, the relationship between hepatic fibrosis in schistosomiasis and dysregulated bilirubin metabolism remains unclear. In this study, we observed a positive correlation between total bilirubin levels and the levels of ALT, AST, LN, and CIV in patients with advanced schistosomiasis. Additionally, we established mouse models at different time points following S. japonicum infection. As the infection time increased, liver fibrosis escalated, while liver UGT1A1 consistently exhibited a low expression, indicating impaired glucuronidation of bilirubin metabolism in mice. In vitro experiments suggested that SEA may be a key inhibitor of hepatic UGT1A1 expression after schistosome infection. Furthermore, a high concentration of bilirubin activated the NF-κB signaling pathway in L-O2 cells in vitro. These findings suggested that the dysregulated glucuronidation of bilirubin caused by S. japonicum infection may play a significant role in schistosomiasis liver fibrosis through the NF-κB signaling pathway.
Silibinin’s Effects against Methotrexate-Induced Hepatotoxicity in Adjuvant-Induced Arthritis Rat Model
Authors: Khawaja Ghada , El-Orfali Youmna
Published: March 2024

Methotrexate (MTX) is the first drug of choice to treat several diseases, including rheumatoid arthritis. However, its administration is accompanied by severe side effects, most commonly hepatotoxicity. Hence, alternative therapies with a lower toxicity and fewer side effects are needed. This study aimed to investigate the antioxidant and hepatoprotective effects of silibinin (SIL, natural agent) against MTX-induced hepatotoxicity in an adjuvant-induced arthritis (AIA) rat model. Arthritic rats were treated with SIL (100 mg/kg) and/or methotrexate (2 mg/kg). Non-arthritic rats, arthritic untreated rats, and arthritic rats who received the vehicle were followed in parallel. SIL alleviated the systemic consequences of arthritis by restoring lost weight, decreasing the erythrocyte sedimentation rate, and ameliorating joint damage, which was evident both micro- and macroscopically. Additionally, SIL prevented the histopathological alterations in the liver and significantly reduced the liver damage caused by MTX and AIA, as shown by a decrease in the markers of liver damage (ALT and AST). Furthermore, SIL relieved the oxidative stress induced by AIA and MTX in liver tissue by decreasing the lipid peroxidation (MDA) levels and enhancing the antioxidant defense system (GSH levels; catalase and superoxide dismutase (SOD) activities). In conclusion, our results suggest that SIL is a potent antioxidant and hepatoprotective agent in arthritic rats. It markedly attenuated the progression and severity of the arthritic disease and eased the oxidative stress in liver tissue by improving the pro-oxidant/antioxidant balance.
Quantifying the Impact of Uneventful LASIK on the Cornea
Authors: Jadnanansing Arieke , Kreps Elke O. , Claerhout Ilse , Kestelyn Philippe G. , Consejo Alejandra
Published: March 2024

This study investigates the changes in the optical properties of the cornea after laser in situ keratomileusis (LASIK) based on Scheimpflug light intensity distribution. Retrospective data from patients who had undergone uneventful LASIK surgery were eligible. Scheimpflug images obtained with the Pentacam (6.10r59) HR device prior to the LASIK treatment, 3 months afterwards and (in a subset of patients) 1 year after treatment were exported for analysis of corneal transparency in different depths and regions. Corneal segmentation and statistical modeling of the pixel brightness distribution were performed for each image. The intensity of the pixels corresponding to the central 8 mm of the cornea was statistically modeled using the Weibull function, yielding two derived parameters: the scale parameter (α, representing corneal transparency) and the shape parameter (β, representing corneal tissue homogeneity). Additionally, the same analysis was carried out within the flap area (central 3.5 mm). A total of 90 patients were included. No statistically significant changes were observed in parameter α (p > 0.05). Parameter β exhibited significantly lower values at both the 3-month follow-up (in the anterior cornea and stroma, p < 0.05) and 1-year follow-up (in all depths, p < 0.05). In conclusion, the cornea demonstrated significantly lower tissue homogeneity following uncomplicated LASIK surgery, but overall corneal transparency remained unchanged.
Biochar and Manure Co-Application Increases Rice Yield in Low Productive Acid Soil by Increasing Soil pH, Organic Carbon, and Nutrient Retention and Availability
Authors: Liang Dong , Ning Yunwang , Ji Cheng , Zhang Yongchun , Wu Huashan , Ma Hongbo , Zhang Jianwei , Wang Jidong
Published: March 2024

In recent years, overuse of chemical fertilization has led to soil acidification and decreased rice yield productivity in southern China. Biochar and manure co-application remediation may have positive effects on rice yield and improve acid paddy soil fertility. This study was conducted to understand the effects of co-application of wood biochar and pig manure on rice yield and acid paddy soil quality (0–40 cm soil layers) in a 5-year field experiment. The experiment consisted of six treatments: no biochar and no fertilizer (CK); biochar only (BC); mineral fertilizer (N); mineral fertilizer combined with biochar (N + BC); manure (25% manure N replacing fertilizer N) combined with mineral fertilizer (MN); and manure combined with mineral fertilizer and biochar (MN + BC). Total nitrogen application for each treatment was the same at 270 kg nitrogen ha−1y−1, and 30 t ha−1 biochar was added to the soil only in the first year. After five years, compared with N treatments, N + BC, MN, and MN + BC treatments increased the rice yield rate to 2.8%, 4.3%, and 6.3%, respectively, by improving soil organic matter, total nitrogen, and available phosphate under a 0–40 cm soil layer. MN + BC had the strongest resistance to soil acidification among all the treatments. The interaction between fertilizers and biochar application was significant (p < 0.05) in rice yield, soil electrical conductivity (10–20 cm), and soil available phosphate (20–40 cm). Principal component analysis indicated that the effect of manure on soil property was stronger than that of biochar in the 0–40 cm soil layer. The overall rice yield and soil fertility decreased in the order of biochar + mineral fertilizer + manure > mineral fertilizer + manure > biochar + mineral fertilizer > mineral fertilizer > biochar > control. These results suggest that biochar and manure co-application is a long-term viable strategy for improving acid soil productivity due to its improvements in soil pH, organic carbon, nutrient retention, and availability.
Characterization of Low- and High-Velocity Responses of Basalt–Epoxy and Basalt–Elium Composites
Authors: Llanos Jesse Joseph , Wang Ke , Taheri Farid
Published: March 2024

Currently, fiber-reinforced polymer composites (FRPs) used for demanding structural applications predominantly utilize carbon, glass, and aramid fibers embedded in epoxy resin, albeit occasionally polyester and vinyl ester resins are also used. This study investigates the feasibility of employing recyclable and sustainable materials to formulate a composite suitable for load-bearing structural applications, particularly in scenarios involving low-velocity and high-velocity impacts (LVIs and HVIs, respectively). The paper presents a comparative analysis of the performance of basalt–Elium, a fully recyclable, sustainable, and environmentally friendly composite, with an epoxy-based counterpart. Moreover, an accurate and reliable numerical model has been developed and introduced through which the response of these composites can be examined efficiently and accurately under various loading states. The results of this investigation demonstrate the viability of the basalt–elium composite as a fully recyclable and sustainable material for crafting efficient and lightweight composites. Additionally, the accurately developed finite element model presented here can be used to assess the influence of several parameters on the composite, thereby optimizing it for a given situation.
On Designing a New Control Chart Using the Generalized Conway–Maxwell–Poisson Distribution to Monitor Count Data
Authors: Mustafa Fakhar , Sherwani Rehan Ahmad Khan , Raza Muhammad Ali , Darwish Jumanah Ahmed
Published: March 2024

Many researchers employed Poisson distribution-based control charts to monitor count data. Nevertheless, these charts can handle count data that deviate from the Poisson assumption of equal mean and variance. This paper suggests a new control chart (CC) that uses the generalized Conway–Maxwell–Poisson (GCOMP) distribution, which can deal with count data that have different levels of dispersion and zero-inflation (ZI). The proposed chart is designed considering the total number of counts. The main advantage of this study is that it pays attention to the tails of the count data when monitoring the process. The performance is measured by the average run length using L control limits at different sample sizes and parametric settings. The findings demonstrate that, for count data with varying tail behaviors, the proposed chart performs better compared to existing CCs. ZI count data can also be monitored with the proposed chart. The proposed chart can be applied in a variety of fields, as verified by the examples provided in this paper.
Response of Ecosystem Carbon–Water Fluxes to Extreme Drought in West Asia
Authors: Alsafadi Karam , Bashir Bashar , Mohammed Safwan , Abdo Hazem Ghassan , Mokhtar Ali , Alsalman Abdullah , Cao Wenzhi
Published: March 2024

Global warming has resulted in increases in the intensity, frequency, and duration of drought in most land areas at the regional and global scales. Nevertheless, comprehensive understanding of how water use efficiency (WUE), gross primary production (GPP), and actual evapotranspiration (AET)-induced water losses respond to exceptional drought and whether the responses are influenced by drought severity (DS) is still limited. Herein, we assess the fluctuation in the standardized precipitation evapotranspiration index (SPEI) over the Middle East from 1982 to 2017 to detect the drought events and further examine standardized anomalies of GPP, WUE, and AET responses to multiyear exceptional droughts, which are separated into five groups designed to characterize the severity of extreme drought. The intensification of the five drought events (based on its DS) increased the WUE, decreased the GPP and AET from D5 to D1, where both the positive and negative variance among the DS group was statistically significant. The results showed that the positive values of standardized WUE with the corresponding values of the negative GPP and AET were dominant (44.3% of the study area), where the AET values decreased more than the GPP, and the WUE fluctuation in this region is mostly controlled by physical processes, i.e., evaporation. Drought’s consequences on ecosystem carbon-water interactions ranged significantly among eco-system types due to the unique hydrothermal conditions of each biome. Our study indicates that forthcoming droughts, along with heightened climate variability, pose increased risks to semi-arid and sub-humid ecosystems, potentially leading to biome restructuring, starting with low-productivity, water-sensitive grasslands. Our assessment of WUE enhances understanding of water-carbon cycle linkages and aids in projecting ecosystem responses to climate change.
Evaluation of GPM IMERG-FR Product for Computing Rainfall Erosivity for Mainland China
Authors: Wang Wenting , Jiang Yuantian , Yu Bofu , Zhang Xiaoming , Xie Yun , Yin Bing
Published: March 2024

Satellite precipitation products (SPPs) have emerged as an alternative to estimate rainfall erosivity. However, prior studies showed that SPPs tend to underestimate rainfall erosivity but without reported bias-correction methods. This study evaluated the efficacy of two SPPs, namely, GPM_3IMERGHH (30-min and 0.1°) and GPM_3IMERGDF (daily and 0.1°), in estimating two erosivity indices in mainland China: the average annual rainfall erosivity (R-factor) and the 10-year event rainfall erosivity (10-yr storm EI), by comparing with that derived from gauge-observed hourly precipitation (Gauge-H). Results indicate that GPM_3IMERGDF yields higher accuracy than GPM_3IMERGHH, though both products generally underestimate these indices. The Percent Bias (PBIAS) is −55.48% for the R-factor and −56.38% for the 10-yr storm EI using GPM_3IMERGHH, which reduces to −10.86% and −32.99% with GPM_3IMERGDF. A bias-correction method was developed based on the systematic difference between SSPs and Gauge-H. A five-fold cross validation shows that with bias-correction, the accuracy of the R-factor and 10-yr storm EI for both SPPs improve considerably, and the difference between two SSPs is reduced. The PBIAS using GPM_3IMERGHH decreases to −0.06% and 0.01%, and that using GPM_3IMERGDF decreases to −0.33% and 0.14%, respectively, for the R-factor and 10-yr storm EI. The rainfall erosivity estimated with SPPs with bias-correction shows comparable accuracy to that obtained through Kriging interpolation using Gauge-H and is better than that interpolated from gauge-observed daily precipitation. Given their high temporal and spatial resolution, and timely updates, GPM_3IMERGHH and GPM_3IMERGDF are viable data products for rainfall erosivity estimation with bias correction.
A Polyvinylpyrrolidone Nanofibrous Sensor Doubly Decorated with Mesoporous Graphene to Selectively Detect Acetic Acid Vapors
Authors: Papa Paolo , Zampetti Emiliano , Molinari Fabricio Nicolas , De Cesare Fabrizio , Di Natale Corrado , Tranfo Giovanna , Macagnano Antonella
Published: March 2024

An original approach has been proposed for designing a nanofibrous (NF) layer using UV-cured polyvinylpyrrolidone (PVP) as a matrix, incorporating mesoporous graphene carbon (MGC) nanopowder both inside and outside the fibers, creating a sandwich-like structure. This architecture is intended to selectively adsorb and detect acetic acid vapors, which are known to cause health issues in exposed workers. The nanocomposite MGC-PVP-NFs layer was fabricated through electrospinning deposition onto interdigitated microelectrodes (IDEs) and stabilized under UV–light irradiation. To enhance the adhesion of MGC onto the surface of the nanocomposite polymeric fibers, the layer was dipped in a suspension of polyethyleneimine (PEI) and MGC. The resulting structure demonstrated promising electrical and sensing properties, including rapid responses, high sensitivity, good linearity, reversibility, repeatability, and selectivity towards acetic acid vapors. Initial testing was conducted in a laboratory using a bench electrometer, followed by validation in a portable sensing device based on consumer electronic components (by ARDUINO®). This portable system was designed to provide a compact, cost-effective solution with high sensing capabilities. Under room temperature and ambient air conditions, both laboratory and portable tests exhibited favorable linear responses, with detection limits of 0.16 and 1 ppm, respectively.
Ancient Chinese Character Recognition with Improved Swin-Transformer and Flexible Data Enhancement Strategies
Authors: Zheng Yi , Chen Yi , Wang Xianbo , Qi Donglian , Yan Yunfeng
Published: March 2024

The decipherment of ancient Chinese scripts, such as oracle bone and bronze inscriptions, holds immense significance for understanding ancient Chinese history, culture, and civilization. Despite substantial progress in recognizing oracle bone script, research on the overall recognition of ancient Chinese characters remains somewhat lacking. To tackle this issue, we pioneered the construction of a large-scale image dataset comprising 9233 distinct ancient Chinese characters sourced from images obtained through archaeological excavations. We propose the first model for recognizing the common ancient Chinese characters. This model consists of four stages with Linear Embedding and Swin-Transformer blocks, each supplemented by a CoT Block to enhance local feature extraction. We also advocate for an enhancement strategy, which involves two steps: firstly, conducting adaptive data enhancement on the original data, and secondly, randomly resampling the data. The experimental results, with a top-one accuracy of 87.25% and a top-five accuracy of 95.81%, demonstrate that our proposed method achieves remarkable performance. Furthermore, through the visualizing of model attention, it can be observed that the proposed model, trained on a large number of images, is able to capture the morphological characteristics of ancient Chinese characters to a certain extent.
Improving Optical Flow Sensor Using a Gimbal for Quadrotor Navigation in GPS-Denied Environment
Authors: Flores Jonathan , Gonzalez-Hernandez Ivan , Salazar Sergio , Lozano Rogelio , Reyes Christian
Published: March 2024

This paper proposes a new sensor using optical flow to stabilize a quadrotor when a GPS signal is not available. Normally, optical flow varies with the attitude of the aerial vehicle. This produces positive feedback on the attitude control that destabilizes the orientation of the vehicle. To avoid this, we propose a novel sensor using an optical flow camera with a 6DoF IMU (Inertial Measurement Unit) mounted on a two-axis anti-shake stabilizer mobile aerial gimbal. We also propose a robust algorithm based on Sliding Mode Control for stabilizing the optical flow sensor downwards independently of the aerial vehicle attitude. This method improves the estimation of the position and velocity of the quadrotor. We present experimental results to show the performance of the proposed sensor and algorithms.
Combined Permutation Tests for Pairwise Comparison of Scale Parameters Using Deviances
Authors: Richter Scott J. , McCann Melinda H.
Published: March 2024

Nonparametric combinations of permutation tests for pairwise comparison of scale parameters, based on deviances, are examined. Permutation tests for comparing two or more groups based on the ratio of deviances have been investigated, and a procedure based on Higgins’ RMD statistic was found to perform well, but two other tests were sometimes more powerful. Thus, combinations of these tests are investigated. A simulation study shows a combined test can be more powerful than any single test.
Gender and Socioeconomic Influences on Ten Pro-Environmental Behavior Intentions: A German Comparative Study
Authors: Schulz Paul , Nicolai Susanne , Tomczyk Samuel , Schmidt Silke , Franikowski Philipp , Stoll-Kleemann Susanne
Published: March 2024

Pro-environmental behaviors (PEBs) such as climate-friendly mobility and eating habits hold great promise in terms of reducing greenhouse gas (GHG) emissions and, thus, are important goals for addressing climate change from a population perspective. Yet, sociodemographic correlates and differences in PEB intentions have to be considered in designing messages and behavior change interventions. This study implemented a quota-sampling survey (N = 979, 511 women, 468 men, age M = 50.4, SD = 17.2) of the German population and found that, overall, participants exhibit strong intentions to engage in various PEBs, with the exception of cycling and adopting a vegetarian diet. Moreover, women displayed higher intentions to engage in PEBs compared to men, particularly in adopting a vegetarian diet. The relationships between socioeconomic status (SES) and PEB intentions, as well as the combined effects of gender and SES, were inconsistent for different PEB intentions. We conclude that on a population level, intention-building interventions are necessary for vegetarianism and cycling, while for the other PEBs, interventions may focus on closing the intention–behavior gap. There is a need to further research the interplay of different PEBs in diverse groups and for interventional studies targeting the discrepancy in eating habits across genders.