bluuebunny/crossref_metadata_2025 huggingface dataset:
Increasing sorghum yields for smallholder farmers in Mali: the evolution towards a context-driven, on-farm, gender-responsive sorghum breeding program
Authors: Isaacs Krista , Weltzien Eva , Some Hermann , Diallo Abdoulaye , Diallo Bocar , Sidibé Mamourou , vom Brocke Kirsten , Samake Bakary , Nebié Baloua , Rattunde Fred W.
Published: May 2024

This case study explores a decades long evolution towards a gender-responsive sorghum breeding program in Mali. With known disparities in men and women’s access to the resources that improve agricultural productivity and evidence that gender roles and responsibilities shape knowledge and preferences about varieties, there is need for methods that support gender-responsive processes in plant breeding programs. Gender-sensitive and gender-responsive approaches in plant breeding may increase varietal options available to diverse end-users, increase adoption, and limit negative impacts on vulnerable populations. We assess a participatory plant breeding program in Mali to identify determinants of gender-responsive breeding programs. The analysis uses a case study methodology that draws upon project reports, theses, articles, and experiential knowledge to understand how the sorghum breeding program transitioned over time. This case study details (a) more than a decade of sorghum breeding activities and research that led to (b) the inclusion of women in participatory plant breeding, culinary tests, and large-scale participatory selection in on-farm trials, reaching hundreds of women each year and (c) iterative co-learning processes to develop preferred sorghum varieties and increase sorghum yields on men and women’s fields. Analyses indicated that collaborations among many institutions on-farm with community actors, research across various disciplines such as agronomy and social sciences, context-specific breeding, and long-term funding were essential to increasing gender sensitivity and responsive in the breeding efforts.
Duck Tembusu virus in North Vietnam: epidemiological and genetic analysis reveals novel virus strains
Authors: Dong Hieu Van , Tran Giang Thi Huong , Vu Tra Thi Thu , Le Ngan Hong Thi , Nguyen Yen Thi Hoang , Rapichai Witsanu , Rattanasrisomporn Amonpun , Boonkaewwan Chaiwat , Bui Dao Anh Tran , Rattanasrisomporn Jatuporn
Published: May 2024

Tembusu virus (TMUV) is an important infectious disease, causing economic losses in duck production. Since the first report of TMUV infection in Vietnam in 2020, the disease has persisted and affected poultry production in the country. This study conducted epidemiological and genetic characterization of the viral strains circulating in north Vietnam based on 130 pooled tissue samples collected in six provinces/cities during 2021. The TMUV genome was examined using conventional PCR. The results indicated that 21 (16.15%) samples and 9 (23.68%) farms were positive for the viral genome. The positive rate was 59.26% for ducks at ages 2–4 weeks, which was significantly higher than for ducks at ages >4 weeks and < 2 weeks. Genetic analysis of the partial envelope gene (891 bp) sequences indicated that the five Vietnamese TMUVs shared 99.55–100% nucleotide identity, while the rates were in the range 99.59–100% based on the pre-membrane gene sequences (498 bp). The five Vietnamese TMUV strains obtained formed a novel single subcluster. These strains were closely related to Chinese strains and differed from the vaccine strain, suggesting that Vietnamese TMUV strains were field viruses. It needs to be further studied on vaccine development to prevent effects of TMUV infection on poultry production across Vietnam.
Carboplatin restricts peste des petits ruminants virus replication by suppressing the STING-mediated autophagy
Authors: Zhang Rui , Hu Zhanying , Wei Dingcheng , Li Ruizhe , Li Yanmin , Zhang Zhidong
Published: May 2024

Peste des petits ruminants virus (PPRV) is a morbillivirus that causes the acute and highly pathogenic infectious disease peste des petits ruminants (PPR) in small ruminants and poses a major threat to the goat and sheep industries. Currently, there is no effective treatment for PPRV infection. Here, we propose Carboplatin, a platinum-based regimen designed to treat a range of malignancies, as a potential antiviral agent. We showed that Carboplatin exhibits significant antiviral activity against PPRV in a cell culture model. The mechanism of action of Carboplatin against PPRV is mainly attributed to its ability to block STING mediated autophagy. Together, our study supports the discovery of Carboplatin as an antiviral against PPRV and potentially other closely related viruses, sheds light on its mode of action, and establishes STING as a valid and attractive target to counteract viral infection.
Decentralized Output-Feedback Adaptive Event-Triggered Control for Interconnected Nonlinear Delay Systems with Actuator Failures
Authors: He Wenmin , Liu Yu , Zhang Quanling
Published: May 2024

This paper investigates decentralized adaptive event-triggered fault-tolerant control for interconnected nonlinear delay systems with actuator failures. The actuator failures suffered include loss of effectiveness and bias faults. A control scheme based on the K-filter is proposed, which effectively compensates for the effects of unknown actuator failures. A hyperbolic tangent function and neural network are introduced to approximate the unknown interconnection function and nonlinear delay function. By introducing the dynamic surface control method, the “explosion of complexity” issue is addressed. Furthermore, our proposed controller can ensure that all states of the corresponding closed-loop system are semi-globally uniformly ultimately bounded and that the tracking error can converge to a small neighborhood of zero. Meanwhile, Zeno behavior can be effectively avoided. Finally, the validity of the proposed control scheme is verified using a simulation example.
Fintech: Evidence of the Urgent Need to Improve Financial Literacy in Portugal
Authors: Costa Mariana , Au-Yong-Oliveira Manuel , Moreira Ana
Published: May 2024

Fintech has revolutionized the financial sector, providing a new way of providing banking services. Since Fintech can provide the same services as traditional banks but entirely online, it is a competitor. As a result, consumers’ relationships with banking have inevitably changed, and it is therefore relevant to analyze these changes. The main objective of this study is to understand people’s perceptions of Fintech, their level of knowledge about it, and the impact of its emergence on traditional banking. The study sample consisted of 174 participants. A quantitative methodology was used to test the hypotheses formulated. The results show that participants who know about Fintech and perceive it as safe have a greater intention of changing banks. On the other hand, they perceive that supervision and regulation in traditional banks is higher than in Fintech. Among the reasons for becoming a Fintech customer, the most mentioned were lower costs and the fact that they provide greater convenience and ease of use. It will be in Fintech’s interest to continue working with regulators so that the sector makes progress in this area and consumers can recognize greater equality between traditional banks and Fintech in the future.
Enhancing Small Object Detection in Aerial Images: A Novel Approach with PCSG Model
Authors: An Kang , Duanmu Huiping , Wu Zhiyang , Liu Yuqiang , Qiao Jingzhen , Shangguan Qianqian , Song Yaqing , Xu Xiaonong
Published: May 2024

Generalized target detection algorithms perform well for large- and medium-sized targets but struggle with small ones. However, with the growing importance of aerial images in urban transportation and environmental monitoring, detecting small targets in such imagery has been a promising research hotspot. The challenge in small object detection lies in the limited pixel proportion and the complexity of feature extraction. Moreover, current mainstream detection algorithms tend to be overly complex, leading to structural redundancy for small objects. To cope with these challenges, this paper recommends the PCSG model based on yolov5, which optimizes both the detection head and backbone networks. (1) An enhanced detection header is introduced, featuring a new structure that enhances the feature pyramid network and the path aggregation network. This enhancement bolsters the model’s shallow feature reuse capability and introduces a dedicated detection layer for smaller objects. Additionally, redundant structures in the network are pruned, and the lightweight and versatile upsampling operator CARAFE is used to optimize the upsampling algorithm. (2) The paper proposes the module named SPD-Conv to replace the strided convolution operation and pooling structures in yolov5, thereby enhancing the backbone’s feature extraction capability. Furthermore, Ghost convolution is utilized to optimize the parameter count, ensuring that the backbone meets the real-time needs of aerial image detection. The experimental results from the RSOD dataset show that the PCSG model exhibits superior detection performance. The value of mAP increases from 97.1% to 97.8%, while the number of model parameters decreases by 22.3%, from 1,761,871 to 1,368,823. These findings unequivocally highlight the effectiveness of this approach.
Identification and Evaluation of Celery Germplasm Resources for Salt Tolerance
Authors: Wu Limei , Du Jiageng , Zhang Yidan , Xue Yuqin , Jiang Chengyao , Lu Wei , Zheng Yangxia , Zhou Chengbo , Xiong Aisheng , Li Mengyao
Published: May 2024

This study evaluated the salt tolerance in 40 celery germplasm resources to clarify the different salt tolerances of celery germplasm. A gradient treatment with different concentrations of NaCl solutions (100, 200, and 300 mmol·L−1) was used to simulate salt stress. After 15 days of salt treatment, 14 indicators related to plant growth, physiology, and biochemistry were determined. The results showed that different celery varieties responded differently to salt stress. Notably, there were significant variations in below-ground dry weight, root–crown ratio, antioxidant enzyme activity, and soluble protein content among the accessions under salt stress. Principal component analysis was used to identify important indices for evaluating salt tolerance, including plant height, spread, content of soluble protein, and so on. A comprehensive evaluation was conducted utilizing the salt damage index, principal component analysis, affiliation function analysis, and cluster analysis. The 40 celery germplasms were classified into five highly salt-tolerant, seven salt-tolerant, fifteen moderately salt-tolerant, nine salt-sensitive, and four highly salt-sensitive germplasms. SHHXQ, MXKQ, XBQC, XQ, and TGCXBQ were highly salt-tolerant germplasms, and BFMSGQ, HNXQ, ZQ, and MGXQW were highly salt-sensitive germplasms. The results of this study provide a reference for the variety of celery cultivation in saline areas and lay a foundation for the selection and breeding of salt-tolerant varieties of celery.
Effects of Maize/Peanut Intercropping and Nitrogen Fertilizer Application on Soil Fungal Community Structure
Authors: Zhang Yongyong , Zhao Fengyan , Feng Chen , Bai Wei , Zhang Zhe , Cai Qian , Sun Zhanxiang , Feng Liangshan
Published: May 2024

Maize/peanut intercropping may improve soil health through reducing nitrogen (N) fertilization. However, the effects of maize/peanut intercropping combined with reduced N fertilization on the soil fungal community structure have not been well reported. Using a long-term localized micro-zone experiment, we investigated the combined effects of intercropping and N fertilizer application on soil fungal community diversity and composition. Three cropping patterns (maize/peanut intercropping, maize monoculture, and peanut monoculture) and three N application levels (0 kg·hm−2, 150 kg·hm−2, and 300 kg·hm−2) were assessed. The results showed that the total numbers of fungal species and unique species (operational taxonomic units, OTUs) in both maize and peanut soils tended to first increase and then decrease with increasing N application. Compared with monoculture, the numbers of total OTUs and unique OTUs in intercropped maize soil decreased by 4.14% and 12.79%, respectively, but the total numbers of OTUs and unique OTUs in peanut soil increased by 1.08% and 3.78%, respectively. With increasing N application, the soil fungal Ace and Chao indices of maize soil first increased and then decreased, while the fungal Shannon, Ace, and Chao indices of peanut soil decreased. Compared with the monoculture system, intercropping significantly reduced the maize soil fungal Ace and Chao indices but increased the peanut soil fungal Shannon, Ace, and Chao indices. Nitrogen application and intercropping significantly altered the fungal community structure of maize soil, while N application had no significant effect on the fungal community structure of peanut soil, though intercropping significantly changed the fungal community structure of peanut soil. At the phylum level, Ascomycota, Basidiomycota, Mortierellomycota, unclassified_k_Fungi, and Chytridiomycota were the dominant taxa. Redundancy analysis (RDA) showed that soil nitrate (NO3−) content was the main environmental factor shaping the soil fungal community. In conclusion, excessive N fertilization (300 kg·hm−2) can reduce soil fungal community diversity; maize/peanut intercropping reversed the negative effect of N application on fungal community of peanut soil, but not that of maize soil. Soil NO3− content is the primary environmental driver of soil fungal communities.
Quantifying the Environmental Impact of Private and Commercial Pilot License Training in Canada
Authors: Rizvi Syed A. Q. , Kearns Suzanne , Cao Shi
Published: May 2024

As the global aviation sector expands to accommodate increasing air travel demand, the subsequent rise in flights exacerbates carbon dioxide (CO2) emissions, challenging the sector’s environmental sustainability. Targeting net-zero emissions by 2050, international aviation agencies are stressing the imperative of reducing emissions directly at their source. While the literature provides abundant estimates of aviation emissions from airline flights, there has been a lack of work aimed at quantifying CO2 emissions specific to the general aviation sector. This study investigates CO2 emissions attributed to the pilot training sub-sector within Canada’s general aviation sector. It specifically examines the initial phase of pilot training, known as ab initio training, extending through to the attainment of a commercial pilot license. Utilizing a mathematical framework alongside assumptions, combined with data on license issuances over a 23-year period, it estimated that each hour of flight training emits about 70.4 kg of CO2, varying between 44.9 kg and 94.9 kg per hour. Annual CO2 emissions from Canada’s ab initio pilot training are estimated at approximately 30,000 tons, with a possible range of 19,000 to 40,000 tons. The study also explores mitigation opportunities, such as flight simulation training devices and electric aircraft. Though focusing on Canada’s ab initio pilot training, the findings have international relevance.
Thinking Outside the Box: Indirect Myc Modulation in Canine B-Cell Lymphoma
Authors: Licenziato Luca , Mazzone Eugenio , Tarantelli Chiara , Accornero Paolo , Rinaldi Andrea , Divari Sara , Leung Wilfred , Webb Suzin , De Maria Raffaella , Aresu Luca
Published: May 2024

B-cell lymphomas (BCL) is the most frequent hematological cancer in dogs. Treatment typically consists of chemotherapy, with CHOP-based protocols. However, outcome remains generally poor, urging the exploration of new therapeutic strategies with a targeted approach. Myc transcription factor plays a crucial role in regulating cellular processes, and its dysregulation is implicated in numerous human and canine malignancies, including canine BCL (cBCL). This study aims to evaluate the efficacy of indirectly inhibiting Myc in cBCL using BI2536 and MZ1 compounds in two in vitro models (CLBL-1 and KLR-1201). Both BI2536 and MZ1, alone and combined, affected cell viability in a significant concentration- and time-dependent manner. Western Blot revealed an upregulation of PLK1 expression in both cell lines upon treatment with BI2536, in association with a reduction in c-Myc protein levels. Conversely, MZ1 led to a decrease in its primary target, BRD4, along with a reduction in c-Myc. Furthermore, BI2536, both alone and in combination with MZ1, induced larger transcriptomic changes in cells compared to MZ1 alone, primarily affecting MYC target genes and genes involved in cell cycle regulation. These data underscore the potential role of Myc as therapeutic target in cBCL, providing a novel approach to indirectly modulate this molecule.
Effects of the Addition of Trichoderma reesei Cellulase to Broiler Chicken Diets for a 21-Day Period
Authors: Perim Francisco dos Santos , da Silva Weslane Justina , de Souza Dênia Oliveira , Ulhoa Cirano José , Rezende Camila Ferreira , dos Santos Ludmilla Faria , dos Santos Fabiana Ramos , Silva Fabiano Guimarães , Minafra Cibele Silva
Published: May 2024

The cellulose present in the cell wall of vegetables prevents the greater release of nutrients to the animal. Therefore, the use of the cellulase enzyme is a viable strategy as it is capable of breaking cellulose bonds, releasing nutrients such as glucose, increasing dietary energy, and thus improving the productive performance of birds. Trichoderma reesei is efficient in the production of cellulase, which is produced via submerged fermentation followed by purification, formulation, and drying. Therefore, an experiment was carried out using 240 male broilers of the Cobb-500® lineage to verify the effects resulting from the addition of powdered (500 g/t and 1000 g/t) and liquid (500 mL/t) cellulase over a period of 1 to 21 days. A completely randomized experimental design was used, consisting of four treatments with six replications and ten birds per replication that were housed in an experimental cage. It was observed that performance and digestibility results were significantly different with cellulase supplementation. Also, the relative weight of the large intestine in the period between one and seven days increased when cellulase was added at 1000 g/t. In the period of between eight and 14 days of life, the birds that consumed only the basal diet obtained higher levels of liver protein than those that received the treatments with the addition of the enzyme. However, 15 and 21 days, the consumed feed effect did not occur between thus, it is not conclusive whether hepatotoxicity occurs with the addition of cellulase. For the blood parameters, at 21 days, the diets with added cellulase were not significantly different regarding electrolytes. It was concluded that this cellulase produced by Trichoderma reesei can be included in the animals’ diet.
Puppy Socialisation Experiences in Relation to Age and COVID-19 Lockdown Restrictions in the UK and ROI
Authors: Kinsman Rachel H. , Jim Hoi-Lam , Casey Rachel A. , Ruiz-Izaguirre Eliza , Owczarczak-Garstecka Sara , Cooper Ben , Tasker Séverine , Murray Jane K.
Published: May 2024

Limited socialisation can contribute to the development of undesirable dog behaviours. The COVID-19 lockdown potentially limited socialisation opportunities, which may negatively impact the future behaviour of puppies raised during lockdown. Data were gathered from longitudinal study participants in the United Kingdom/Republic of Ireland via multiple questionnaires between May 2016 and November 2022. The impact of age and lockdown phase (pre-, during, and post-) on the types of socialisation experiences of 8-to-19-week-old puppies and the recency of socialisation experiences of approximately 6-month-old puppies were examined. Puppies under 19-weeks had significantly more types of socialisation experiences (from a predefined list) as they aged, and pre-lockdown compared to post-lockdown, but not between other lockdown phases. Most 6-month-old puppies had met a new adult or dog outside the household, a familiar dog, and/or a child within the last 1–7 days, and this was similar between lockdown phases. During lockdown, 6-month-old puppies experienced longer periods between meeting a new adult in their home. Puppies were hypothesised to have had fewer experiences during lockdown, but this was not found. However, the quantity and quality of these experiences may have been affected. Future research within this longitudinal study will explore relationships between the timing and type of experiences had by puppies and their subsequent behaviour.
Ancient Diseases in Vertebrates: Tumours through the Ages
Authors: Garcês Andreia , Pires Isabel , Garcês Sara
Published: May 2024

Paleo-oncology studies neoplastic diseases in fossilised animals, including human remains. Recent advancements have enabled more accurate diagnoses of ancient pathologies despite the inherent challenges in identifying tumours in fossils—such as the rarity of well-preserved specimens, the predominance of bone remains, and the difficulty in distinguishing neoplastic from non-neoplastic lesions. This study compiles reports of tumours in fossilised animals, highlighting that neoplasms are present in a wide range of vertebrates and drawing comparisons to modern instances of similar diseases. The findings underscore the multifactorial aetiology of tumours, which involves genetic, environmental, and lifestyle factors, and suggest that tumours have been around for at least 350 million years.
Colonization by Extended-Spectrum β-Lactamase-Producing Enterobacterales and Bacteremia in Hematopoietic Stem Cell Transplant Recipients
Authors: Gonçalves Luiza Arcas , Anjos Beatriz Barbosa , Tavares Bruno Melo , Marchi Ana Paula , Côrtes Marina Farrel , Higashino Hermes Ryoiti , de Carvalho Moraes Bruna del Guerra , Bampi José Victor Bortolotto , Pinheiro Liliane Dantas , Spadao Fernanda de Souza , Rocha Vanderson , Guimarães Thais , Costa Silvia Figueiredo
Published: May 2024

Background: Assessing the risk of multidrug-resistant colonization and infections is pivotal for optimizing empirical therapy in hematopoietic stem cell transplants (HSCTs). Limited data exist on extended-spectrum β-lactamase-producing Enterobacterales (ESBL-E) colonization in this population. This study aimed to assess whether ESBL-E colonization constitutes a risk factor for ESBL-E bloodstream infection (BSI) and to evaluate ESBL-E colonization in HSCT recipients. Methods: A retrospective analysis of ESBL-E colonization and BSI in HSCT patients was conducted from August 2019 to June 2022. Weekly swabs were collected and cultured on chromogenic selective media, with PCR identifying the β-lactamase genes. Pulsed-field gel electrophoresis (PFGE) and whole-genome sequencing (WGS) assessed the colonizing strains’ similarities. Results: Of 222 evaluated HSCT patients, 59.45% were colonized by ESBL-E, with 48.4% at admission. The predominant β-lactamase genes were blaTEM (52%) and blaSHV (20%). PFGE analysis did not reveal predominant clusters in 26 E. coli and 15 K. pneumoniae strains. WGS identified ST16 and ST11 as the predominant sequence types among K. pneumoniae. Thirty-three patients developed thirty-five Enterobacterales-BSIs, with nine being third-generation cephalosporin-resistant. No association was found between ESBL-E colonization and ESBL-BSI (p = 0.087). Conclusions: Although the patients presented a high colonization rate of ESBL-E upon admission, no association between colonization and infection were found. Thus, it seems that ESBL screening is not a useful strategy to assess risk factors and guide therapy for ESBL-BSI in HSCT-patients.
Dung Beetle Optimized Fuzzy PID Algorithm Applied in Four-Bar Target Temperature Control System
Authors: Cao Wenxiao , Liu Zilu , Song Hongfei , Li Guoming , Quan Boyu
Published: May 2024

With the widespread application of infrared thermal imagers in various fields, the demand for thermal imagers and their performance parameter testing equipment has increased significantly. There are particularly high demands on the detection accuracy of minimum resolvable temperature difference (MRTD) testers. Traditional MRTD testers have an issue with the four-bar target temperatures being easily affected by the external environment, resulting in non-uniform temperatures and imprecise detection results. This paper proposes an improvement to the four-bar targets by making them temperature-controllable. Temperature is controlled by installing thermoelectric coolers (TECs) and thin-film platinum resistors at the center and periphery of the four-bar targets with different spatial frequencies. The dung beetle algorithm is used to optimize fuzzy PID parameters to regulate the TEC’s heating and cooling, improving the overall temperature uniformity of the four-bar targets. Temperature simulations of the four-bar targets were conducted on the COMSOL platform, with the control part simulated on the Simulink platform. The simulation results show that, compared to traditional PID, the fuzzy PID controller reduces overshoot by approximately 3.6%, although the system still exhibits mild oscillations. The fuzzy PID controller optimized by the dung beetle optimization (DBO) algorithm, in comparison to standard fuzzy PID, reduces the settling time by about 40 s and lowers overshoot by around 7%, with oscillations in the system nearly disappearing. Comparing the fuzzy PID optimized by the particle swarm optimization (PSO) algorithm with the fuzzy PID optimized by the DBO algorithm, the DBO-based controller shows shorter rise and settling times, further illustrating the superiority of the fuzzy PID control optimized by the dung beetle algorithm. This provides a theoretical foundation for improving the accuracy of MRTD detector measurements. Finally, experimental verification was carried out. The experimental results indicate that DBO (drosophila-based optimization) has significant advantages, and its optimized results are closer to the actual values.
Evaluation and SAR Analysis of Low Frequency and Broadband Electric Field Exposure Measurement Values in the Home Environment
Authors: Mutlu Mustafa
Published: May 2024

In this study, the effective value of low-frequency (50 Hz) and high-frequency (700–2500 MHz) broadband electric field exposures was measured for 24 h in four different selected environments in a house. All the statistical values of 1000 data points recorded with two measuring devices for 24 h in each environment are calculated, the most appropriate curves are fitted to the data, and the curves are plotted by expressing their changes with respect to time. All statistical and parametric values of the density and cumulative probability functions of the curves are calculated and plotted. In broadband measurements, the broadband is divided into fifteen sub-bands, but the data are available in only six of these sub-bands. The six-layer human head model is created by using the middle frequencies of the six sub-bands used, and taking the permittivity (εr) and conductivity (σ) of each layer into consideration. The specific absorption rate (SAR) value in the brain is calculated by using the total transmission coefficient of six layers. The SAR value surrounding the head is obtained. These SAR values are interpreted by considering the Federal Communications Commission (FCC) and the Community European (CE) occupational and general public SAR limits.
Purification, Characterization and Antifungal Activity of the Aspergillus niveus Chitinase Produced Using Shrimp Shells
Authors: Ornela Pedro Henrique , Guimarães Luis Henrique Souza
Published: May 2024

Chitinases are biotechnologically relevant enzymes that can be applied in such different sectors as pharmaceutical, food, environmental management, the biocontrol of pests and in the paper and cellulose industry. Microorganisms as filamentous fungi are the most important source of these biomolecules. The fungus Aspergillus niveus produces extracellular chitinase when cultured under submerged fermentation using shrimp shells, a residue generated by the fish industry, as a carbon source, for 96 h at 30 °C and 100 rpm. The particle size and concentration of the shrimp shells affected enzyme production. The chitinase was purified until electrophoretic homogeneity through the use of a Sephadex G-100 chromatographic column. It is a monomeric glycoprotein with a molecular mass of 47 kDa estimated using SDS-PAGE and 49.3 kDa determined using gel filtration. The carbohydrate content was 22.8%. The best temperature and pH for enzyme activity were 65 °C and 6.0, respectively. Approximately 80% of the enzymatic activity was preserved at pH 4.0 and 5.0 for 48 h, and the half-life (t50) was maintained for 48 h at 40 °C. Salts, EDTA and β-mercaptoethanol did not affect chitinase activity significantly, but organic solvents reduced it. The kinetic parameters determined using p-NPGlycNac were Km of 2.67 mmol L−1, Vmax of 12.58 U mg of protein−1, Kcat of 2.47 s−1 and K cat/Km of 0.93 s−1 mmol L−1. The A. niveus chitinase inhibited the growth of all fungal strains used, especially Trichoderma harzianum (MIC = 22.4 μg mL−1) and Penicillium purpurogenum (MIC = 11.2 μg mL−1). The chitinase produced by A. niveus presented interesting characteristics that indicate its potential of application in different areas.
A German DJ, Postmodern Dreams, and the Ambivalent Politics of East–West Exchange at the First Exhibition of Approximate Art in Riga, April 1987
Authors: Karnes Kevin C.
Published: May 2024

Organized as part of the annual Art Days festival in the capital of the Latvian SSR, the First Exhibition of Approximate Art comprised a cacophonous and provocative mashup of music, dance, performance art, and design. At the center of the event was a demonstration of mixing and scratching records by Maximilian Lenz, also known as Westbam, one of the leading DJs in West Berlin. Mining archival sources in Berlin and Riga, this article reconstructs the complicated path by which the DJ came to perform at the event. It reveals a surprising network of relations and alliances operating in tandem behind the scenes, featuring a Riga artist dedicated to enacting a vision of postmodern performance in his city, an ambitiously networking émigré Latvian living in exile in West Germany, and a pair of Soviet offices under direct control of the KGB, charged with managing cultural exchanges with the West in hopes of currying sympathies for Soviet culture and policy. Complementing and extending research on the “gaps” and “holes” in the Soviet system that sometimes allowed for the staging of otherwise unacceptable works of art, the story of the First Exhibition of Approximate Art reveals how personal connections and interpersonal networks within even the most highly monitored parts of the system itself—the state security apparatus—could open doors for artistic projects unanticipated and even undesired by the bureaucratic state.
Phenomenology of the Composition of PM2.5 at an Urban Site in Northern France
Authors: Allouche Yamina , Fadel Marc , Ferté Amélie , Verdin Anthony , Ledoux Frédéric , Courcot Dominique
Published: May 2024

In this work, PM2.5 was sampled at Dunkerque, a medium-sized city located in northern France. The mean concentration of PM2.5 during the sampling period was 12.6 ± 9.5 μg·m−3. Samples were analyzed for elemental and organic carbon (EC/OC), water-soluble organic carbon (WSOC), humic-like substances (HULIS-C), water-soluble inorganic ions, and major and trace elements. The origin and the variations of species concentrations were examined using elemental enrichment factors, bivariate polar plot representations, and diagnostic concentration ratios. Secondary inorganic ions were the most abundant species (36% of PM2.5), followed by OC (12.5% of PM2.5). Secondary organic carbon (SOC) concentrations were estimated to account for 52% of OC. A good correlation between SOC and WSOC indicated that secondary formation processes significantly contribute to the WSOC concentrations. HULIS-C also represents almost 50% of WSOC. The determination of diagnostic ratios revealed the influence of anthropogenic emission sources such as integrated steelworks and fuel oil combustion. The clustering of 72 h air masses backward trajectories data evidenced that higher concentrations of PM2.5, OC, and secondary inorganic aerosols were recorded when air masses came from north-eastern Europe and the French continental sector, showing the considerable impact of long-range transport on the air quality in northern France.
Novel Tetrazolium-Based Colorimetric Assay for Helicase nsp13 in SARS-CoV-2
Authors: Pham Triet M. , Howard Morgan G. , Carey Shane M. , Baker Lindsey R. , D’Antonio Edward L.
Published: May 2024

Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is a human pathogenic virus that encodes for a helicase (SC2Hel) that is essential for viral replication. SC2Hel has the ability to unravel dsRNA or dsDNA in an NTP-dependent manner from the 5′ to 3′ directionality. The standard helicase assay from studies involving SARS-CoV and SARS-CoV-2 have relied on the concept of fluorescence resonance energy transfer. Adding to the collection of helicase assays, herein, we have developed a novel tetrazolium-based colorimetric assay system for the detection of ADP that is produced via SC2Hel activity. This SC2Hel assay combines three enzyme-coupled steps involving the ADP-dependent Thermococcus litoralis glucokinase (TlGlcK), Leuconostoc mesenteroides glucose-6-phosphate dehydrogenase (LmG6PDH), and Clostridium kluyveri diaphorase (CkDIA). Iodonitrotetrazolium chloride (INT), a colorimetric tetrazolium reagent, was used in the final step of the assay that converted into INT-formazan during reduction. INT-formazan in the assay’s buffered solution at pH 7.6 exhibited an intense colorimetric response at a wavelength maximum of 505 nm. The assay exhibited excellent performance characteristics as it revealed a Z’ factor of 0.87 and it has the potential to be further adopted into high-throughput screening studies for therapeutic drug discovery research.
Finite Element Analysis of a Rib Cage Model: Influence of Four Variables on Fatigue Life during Simulated Manual CPR
Authors: Jeon Jong Hyeok , Sul Jae Ho , Ko Dae Hwan , Seo Myoung Jae , Kim Sung Min , Lim Hong Seok
Published: May 2024

Cardiopulmonary resuscitation (CPR) is a life-saving technique used in emergencies when the heart stops beating, typically involving chest compressions and ventilation. Current adult CPR guidelines do not differentiate based on age beyond infancy and childhood. This oversight increases the risk of fatigue fractures in the elderly due to decreased bone density and changes in thoracic structure. Therefore, this study aimed to investigate the correlation and impact of factors influencing rib fatigue fractures for safer out-of-hospital manual cardiopulmonary resuscitation (OHMCPR) application. Using the finite element analysis (FEA) method, we performed fatigue analysis on rib cage models incorporating chest compression conditions and age-specific trabecular bone properties. Fatigue life analyses were conducted on three age-specific rib cage models, each differentiated by trabecular bone properties, to determine the influence of four explanatory variables (the properties of the trabecular bone (a surrogate for the age of the subject), the site of application of the compression force on the breastbone, the magnitude of applied compression force, and the rate of application of the compression force) on the fatigue life of the model. Additionally, considering the complex interaction of chest compression conditions during actual CPR, we aimed to predict rib fatigue fractures under conditions simulating real-life scenarios by analyzing the sensitivity and interrelation of chest compression conditions on the model’s fatigue life. Time constraints led to the selection of optimal analysis conditions through the use of design of experiments (DOE), specifically orthogonal array testing, followed by the construction of a deep learning-based metamodel. The predicted fatigue life values of the rib cage model, obtained from the metamodel, showed the influence of the four explanatory variables on fatigue life. These results may be used to devise safer CPR guidelines, particularly for the elderly at a high risk of acute cardiac arrest, safeguarding against potential complications like fatigue fractures.
Recent Advances in Dysuricemia: Toward Optimal Serum Urate Level
Authors: Kurajoh Masafumi , Nakayama Akiyoshi
Published: May 2024

We are pleased to present the Special Issue “Dysuricemia: Recent Advances in Urate Research from Hypouricemia to Hyperuricemia/Gout” [...]
Combined Treatments and Therapies to Cure Spinal Cord Injury
Authors: Guérout Nicolas
Published: May 2024

Traumatic injuries of the spinal cord (SCIs) are still pathologies with a disastrous outcome [...]
Passive Anti-Amyloid Beta Immunotherapies in Alzheimer’s Disease: From Mechanisms to Therapeutic Impact
Authors: Schreiner Thomas Gabriel , Croitoru Cristina Georgiana , Hodorog Diana Nicoleta , Cuciureanu Dan Iulian
Published: May 2024

Alzheimer’s disease, the most common type of dementia worldwide, lacks effective disease-modifying therapies despite significant research efforts. Passive anti-amyloid immunotherapies represent a promising avenue for Alzheimer’s disease treatment by targeting the amyloid-beta peptide, a key pathological hallmark of the disease. This approach utilizes monoclonal antibodies designed to specifically bind amyloid beta, facilitating its clearance from the brain. This review offers an original and critical analysis of anti-amyloid immunotherapies by exploring several aspects. Firstly, the mechanisms of action of these therapies are reviewed, focusing on their ability to promote Aβ degradation and enhance its efflux from the central nervous system. Subsequently, the extensive history of clinical trials involving anti-amyloid antibodies is presented, from initial efforts using first-generation molecules leading to mixed results to recent clinically approved drugs. Along with undeniable progress, the authors also highlight the pitfalls of this approach to offer a balanced perspective on this topic. Finally, based on its potential and limitations, the future directions of this promising therapeutic strategy for Alzheimer’s disease are emphasized.
Molecular Role of Protein Phosphatases in Alzheimer’s and Other Neurodegenerative Diseases
Authors: Hassan Mubashir , Yasir Muhammad , Shahzadi Saba , Chun Wanjoo , Kloczkowski Andrzej
Published: May 2024

Alzheimer’s disease (AD) is distinguished by the gradual loss of cognitive function, which is associated with neuronal loss and death. Accumulating evidence supports that protein phosphatases (PPs; PP1, PP2A, PP2B, PP4, PP5, PP6, and PP7) are directly linked with amyloid beta (Aβ) as well as the formation of the neurofibrillary tangles (NFTs) causing AD. Published data reported lower PP1 and PP2A activity in both gray and white matters in AD brains than in the controls, which clearly shows that dysfunctional phosphatases play a significant role in AD. Moreover, PP2A is also a major causing factor of AD through the deregulation of the tau protein. Here, we review recent advances on the role of protein phosphatases in the pathology of AD and other neurodegenerative diseases. A better understanding of this problem may lead to the development of phosphatase-targeted therapies for neurodegenerative disorders in the near future.
The Emerging Role of Silk Fibroin for the Development of Novel Drug Delivery Systems
Authors: Pollini Mauro , Paladini Federica
Published: May 2024

In order to reduce the toxicological impact on healthy cells and to improve the therapeutic response, many drug delivery systems have been fabricated and analysed, involving the use of different natural and synthetic materials at macro-, micro- and nanoscales. Among the natural materials which have demonstrated a huge potential for the development of effective drug delivery systems, silk fibroin has emerged for its excellent biological properties and for the possibility to be processed in a wide range of forms, which can be compliant with multiple active molecules and pharmaceutical ingredients for the treatment of various diseases. This review aims at presenting silk fibroin as an interesting biopolymer for applications in drug delivery systems, exploring the results obtained in recent works in terms of technological progress and effectiveness in vitro and in vivo.
Aggressive and Withdrawn Behaviour at School through the Lens of Teachers and Peers: A Qualitative Study
Authors: Pallini Susanna , Lonigro Antonia , Barcaccia Barbara , Laghi Fiorenzo , Schneider Barry H.
Published: May 2024

We explored teachers’ understanding of children with aggressive or socially withdrawn behaviour in their classes and we associated our findings with a status of rejected, neglected, or popular, as provided by peer nominations. Five kindergarten and elementary school teachers scored their 143 pupils with the Child Behaviour Checklist for Withdrawal and Aggression. Subsequently, only those children whose scores were 1 standard deviation above the mean for withdrawal or for aggression were included in the final sample (n = 46; Mage = 6.5 years, SD = 1.7; age range = 4–9 years). The final sample included 31 children (21.67%; females = 16) who were assessed as displaying withdrawn behaviour, and 15 (10.48%; females = 5) displaying aggressive behaviour. An open-ended semi-structured interview was administered to teachers, who described children with socially withdrawn behaviour as introverted and untalkative, children with aggressive behaviour as hostile, rule-breaking and highly active, and children with socially withdrawn or aggressive behaviour as isolated, even though different reasons were provided. The results of the sociometric status in children with socially withdrawn or aggressive behaviour are discussed.
The Association between Cyberbullying Victimization and Depression among Children: A Moderated Mediation Model
Authors: Song Kuai , Zhou Feng-Juan , Niu Geng-Feng , Fan Cui-Ying , Zhou Zong-Kui
Published: May 2024

Cyberbullying victimization is becoming more prevalent and adversely affects mental health. This research explores the relationship between the two variables and the underlying mechanism, especially for children, as the impact of mental health in childhood might last a lifetime. Primary school students (N = 344; Mage = 9.90; 43.90% girls) completed self-report questionnaires regarding cyberbullying victimization, self-perceived social competence, optimism, and depression at school. Gender and grade were controlled as covariates. Depression was positively predicted by cyberbullying victimization, while self-perceived social competence played a partially mediating role. In addition, optimism directly and indirectly moderated the effects of cyberbullying victimization on depression. Specifically, the effects were stronger for children with low levels of optimism. Therefore, efforts to enhance children’s self-perceived social competence and optimism may reduce their depression resulting from cyberbullying victimization.
Bearing Performance of Prestressed High-Strength Concrete Pipe Pile Cap Connections under Truncated Pile Conditions
Authors: Liu Yasheng , Guo Zhaosheng , He Wubin , Ge Xinsheng , Wang Jingyue , Zhao Jing
Published: May 2024

To investigate the load-carrying performance of the nodes between tubular piles and bearing platforms, low circumferential reciprocating load foot-scale tests were performed on two truncated PHC B 600 130 tubular piles. The development law of node destruction was explored. The test results revealed that under the action of tensile–bending–shear loading, the bearing concrete in the node area buckled and was damaged, and an articulation point was formed. When the embedment depth increased from 200 mm to 300 mm, the ultimate bearing capacities of the positive and negative nodes increased by 57.60% and 54.60%, respectively. Numerical simulation was used to analyze the bearing capacities of nodes with different types and embedment depths. Formulas for the bearing capacity of the nodes were proposed. Furthermore, two preferred node types were proposed as follows: pipe pile core-filled longitudinal reinforcement anchored to the bearing node and pipe pile body longitudinal reinforcement anchored to the bearing node + pipe pile core-filled longitudinal reinforcement anchored to the bearing node, with preferred embedment depths of 350 mm and 200 mm, respectively.
Shaking Tables Test on Seismic Responses of a Long-Span Rigid-Framed Bridge Considering Traveling Wave Effect and Soil–Structure Interaction
Authors: Yan Xiao-Yu , Cao Shan-Shan , Zhao Zhuo
Published: May 2024

The traveling wave effect and soil–structure interaction have significant influence on the seismic response of large-span bridges with complex site conditions. In this paper, a 1/10 scaled-down large-span rigid-framed bridge model was designed and fabricated, and a shaking tables test considering the traveling wave effect and soil–structure interaction was carried out on a large-scale continuous rigid bridge model by a real-time substructure hybrid test technique. Influences of the traveling wave effect and soil–structure interaction on the seismic responses of the rigid-framed bridge specimen were systematically analyzed with experimental data. The test results showed that when the apparent wave speed was small, the traveling wave effect increased the seismic responses of the rigid-framed bridge. With the increase in apparent wave speed, the structural response under traveling wave excitation and uniform excitation was basically the same. The SSI effect lead to a great change in the seismic input peaks and spectral characters at the bottom of the pier, and increased the seismic responses of the rigid-framed bridge. When both traveling wave and the SSI effect were considered, there was a phase difference in the seismic excitation. The dynamic responses of a continuous rigid-framed bridge could not be simply obtained by superposition of the separate traveling wave effect or SSI effect. Meanwhile, the real-time substructure test method in this paper solved the problems that the traditional soil box experiment cannot be applied to the test of a large-scale model, the soil and bridge structure find it difficult to meet the unified similarity ratio, and the boundary conditions are difficult to simulate accurately.
The Role of Autophagy in Vascular Endothelial Cell Health and Physiology
Authors: Hu Meghan , Ladowski Joseph M. , Xu He
Published: May 2024

Autophagy is a highly conserved cellular recycling process which enables eukaryotes to maintain both cellular and overall homeostasis through the catabolic breakdown of intracellular components or the selective degradation of damaged organelles. In recent years, the importance of autophagy in vascular endothelial cells (ECs) has been increasingly recognized, and numerous studies have linked the dysregulation of autophagy to the development of endothelial dysfunction and vascular disease. Here, we provide an overview of the molecular mechanisms underlying autophagy in ECs and our current understanding of the roles of autophagy in vascular biology and review the implications of dysregulated autophagy for vascular disease. Finally, we summarize the current state of the research on compounds to modulate autophagy in ECs and identify challenges for their translation into clinical use.