bluuebunny/crossref_metadata_2025 huggingface dataset:
Black Oat (Avena strigosa Schreb.) Ontogenesis and Agronomic Performance in Organic Cropping System and Pannonian Environments
Authors: Ugrenović Vladan , Popović Vera , Ugrinović Milan , Filipović Vladimir , Mačkić Ksenija , Ljubičić Nataša , Popović Slobodan , Lakić Željko
Published: January 2021

This study examined the influence of agroecological conditions on the ontogenesis and production yield traits of black oat (Avena strigosa Schreb.). Understanding the agronomic and phenological properties related to productive properties of black oats can enable more successful introduction into new areas of oat cultivation. Field microexperiments were performed during the three consecutive years (2017–2019) on the two localities in South Banat, in Serbia, which differed in soil type: carbonate chernozem and humogley, respectively. The results showed that all investigated traits of oat in the chernozem soil type were higher when compared with measured traits obtained from the humogley soil type. The average value of yields of aboveground biomass, yield and protein content were significantly higher in relation to the humogley, by 13%, 17% and 11%, respectively for all three seasons. Observed by years, all productivity parameters differed significantly, which confirmed that the productivity of the cultivated plants was strongly influenced by different agroecological, soil and climatic conditions in Pannonian environments. The aboveground biomass and grain yield obtained from the humogley soil type indicate that this species can be successfully grown and utilized in production as a cover or fodder crop even in less favorable soil conditions.
Practicing Fair and Sustainable Local Food Systems: Elements of Food Citizenship in the Simeto River Valley
Authors: Rico Mendez Gina , Pappalardo Giusy , Farrell Bryan
Published: January 2021

This paper examines a community-based food system which emerged recently around the Simeto River Valley Agreement (SRA) in Sicily (Italy) through the lens of food citizenship. The concept of food citizenship develops an understanding of how food systems function to ensure that individuals and communities have agency, access, and engagement with their food. It allows for comparative analysis between global/industrial and community/alternative systems. This paper follows a methodological integration between action research and a case study approach. The action research process produced a networked governance structure derived from multiple initiatives which are currently initiating many thematic projects—amongst them, a local food system. Results indicate that formalizing governance structures derived from self-organizing behavior have led to an inclusive platform with a shared vision and goals. The governing structures, however, require continued efforts and capacity to engage collaboratively in implementing their strategic plans. Findings suggest that actors developing a food citizenship-focused system should (1) consider how the governance organizational structure enables fluid communication among members and leads to building trust, (2) seek alternatives to engage youth (especially in rural areas) and promote citizen engagement, and (3) develop strategies to seek technical and programmatic support for initiatives. These three aspects are key features which may be adapted to other such efforts in sustainable and local food systems. The complex networked approach to governance presented here and the shared vision for sustainability are considered key elements in fostering a successful alternative food system with the fundamentals of food citizenship at its core.
Effects of Sorghum Silage in Lactating Buffalo Cow Diet: Biochemical Profile, Milk Yield, and Quality
Authors: Tudisco Raffaella , Morittu Valeria Maria , Musco Nadia , Grossi Micaela , Iommelli Piera , D’Aniello Biagio , Ferrara Maria , Infascelli Federico , Lombardi Pietro
Published: January 2021

The influence of replacing silage with sorghum silage in the diet of dairy buffalo cows on metabolic status and on milk yield, chemical characteristics, and fatty acid profile was studied. Forty dairy buffalo cows were included in the trial and divided into two homogeneous groups (SS, sorghum silage and CS, corn silage). Blood was collected at the end of the trial (120 days), individual milk yield was registered daily. Samples of milk were monthly collected and analyzed for fat, protein, and lactose. Moreover, fatty acid profiles of silages and milk were determined. Buffalo cows fed sorghum silage showed an average milk yield higher than group CS (kg/d 10.120 vs. 9.270; p < 0.05), probably due to the lower lignin content of sorghum silage (31.0 g/kg dry matter (DM)) compared to corn silage (47.0 g/kg DM) and, by consequence, to the higher digestibility of SS diet. The percentage of linoleic acid was significantly higher in milk of group CS (C18:2: 1.27 vs. 2.05%; p < 0.01) due to the higher content of these acids in corn than in sorghum silage. The omega 6/omega 3 ratio was significantly lower in milk from buffalo cows fed sorghum than those fed corn silage (7.8 vs. 12.9; p < 0.01). Serum biochemistry showed no negative effects of the corn replacing with sorghum.
Physicochemical and Antioxidant Capacity of Jujube (Ziziphus jujuba Mill.) at Different Maturation Stages
Authors: Reche Juana , Almansa Maria Soledad , Hernández Francisca , Amorós Asunción , Legua Pilar
Published: January 2021

Jujube is a crop very resistant to drought and salinity, making it an interesting growing alternative in southeastern Spain. The characteristics of five different cultivars of the jujube fruit have been evaluated and classified into four different maturation stages according to the color of the peel, ranging from green in its most immature stage, to white, yellow, and red in its last, more mature stage. This is due in part to the amount of carotenoids and chlorophylls studied, which vary as the fruit matures. The cultivars ‘GAL-E’ and ‘GAL-T’ are the largest in size and weight, followed by ‘MSI’, ‘PSI’, and ‘DAT’, which are the smallest cultivars. The content of phenolic compounds was also analyzed. The antioxidant activity, which was studied by different methods, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH), and ferric reducing antioxidant power (FRAP), showed the highest activity in stages 3 and 4 of jujube fruit. The antioxidant activity studied in the hydrophilic and lipophilic fraction by the ABTS method showed the highest peak in stages 1 and 2. This is an important information to know promising cultivars to be used in future breeding programs. Moreover, the maturation stage is relevant to obtain fruit with a high content of bioactive compounds as well as interesting organoleptic properties.
Why a New Journal on Allergies?
Authors: Rougé Pierre
Published: January 2021

The new journal’s title Allergies (ISSN 2313-5786) answers the titular question because among all the existing journals devoted to allergies, few pretend to be exhaustive and cover all possible aspects of allergology [...]
A Review on Mitigating Fear and Aggression in Dogs and Cats in a Veterinary Setting
Authors: Riemer Stefanie , Heritier Carmen , Windschnurer Ines , Pratsch Lydia , Arhant Christine , Affenzeller Nadja
Published: January 2021

A high proportion of dogs and cats are fearful during veterinary visits, which in some cases may escalate into aggression. Here, we discuss factors that contribute to negative emotions in a veterinary setting and how these can be addressed. We briefly summarise the available evidence for the interventions discussed. The set-up of the waiting area (e.g., spatial dividers; elevated places for cat carriers), tailoring the examination and the treatment to the individual, considerate handling (minimal restraint when possible, avoiding leaning over or cornering animals) and offering high-value food or toys throughout the visit can promote security and, ideally, positive associations. Desensitisation and counterconditioning are highly recommended, both to prevent and address existing negative emotions. Short-term pain from injections can be minimised by using tactile and cognitive distractions and topical analgesics, which are also indicated for painful procedures such as ear cleanings. Recommendations for handling fearful animals to minimise aggressive responses are discussed. However, anxiolytics or sedation should be used whenever there is a risk of traumatising an animal or for safety reasons. Stress-reducing measures can decrease fear and stress in patients and consequently their owners, thus strengthening the relationship with the clients as well as increasing the professional satisfaction of veterinary staff.
Evolution of the Seroprevalence of Pestivirus and Respiratory Viral Infections in Spanish Feedlot Lambs
Authors: Navarro Teresa , Ortín Aurora , Cabezón Oscar , De Las Heras Marcelo , Lacasta Delia , González José María
Published: January 2021

The presence of respiratory viruses and pestiviruses in sheep has been widely demonstrated, and their ability to cause injury and predispose to respiratory processes have been proven experimentally. A longitudinal observational study was performed to determine the seroprevalence of bovine parainfluenza virus type 3 (BPIV-3), bovine respiratory syncytial virus (BRSV), bovine herpesvirus type 1 (BHV-1) and pestiviruses in 120 lambs at the beginning and the end of the fattening period. During this time, the animals were clinically monitored, their growth was recorded, and post-mortem examinations were performed in order to identify the presence of pneumonic lesions in the animals. Seroconversion to all viruses tested except BHV-1 was detected at the end of the period. Initially, BPIV-3 antibodies were the most frequently found, while the most common seroconversion through the analysed period occurred to BRSV. Only 10.8% of the lambs showed no detectable levels of antibodies against any of the tested viruses at the end of the survey. In addition, no statistical differences were found in the presentation of respiratory clinical signs, pneumonic lesions nor in the production performance between lambs that seroconverted and those which did not, except in the case of pestiviruses. The seroconversion to pestiviruses was associated with a reduction in the final weight of the lambs.
Automatic Assessment of Keel Bone Damage in Laying Hens at the Slaughter Line
Authors: Jung Lisa , Nasirahmadi Abozar , Schulte-Landwehr Jan , Knierim Ute
Published: January 2021

Keel bone damage (KBD) can be found in all commercial laying hen flocks with a wide range of 23% to 69% of hens/flock found to be affected in this study. As KBD may be linked with chronic pain and a decrease in mobility, it is a serious welfare problem. An automatic assessment system at the slaughter line could support the detection of KBD and would have the advantage of being standardized and fast scoring including high sample sizes. A 2MP stereo camera combined with an IDS imaging color camera was used for the automatic assessment. A trained human assessor visually scored KBD in defeathered hens during the slaughter process and compared results with further human assessors and automatic recording. In a first step, an algorithm was developed on the basis of assessments of keel status of 2287 hens of different genetics with varying degrees of KBD. In two optimization steps, performance data were calculated, and flock prevalences were determined, which were compared between the assessor and the automatic system. The proposed technique finally reached a sensitivity of 0.95, specificity of 0.77, accuracy of 0.86 and precision of 0.81. In the last optimization step, the automatic system scored on average about 10.5% points lower KBD prevalences than the human assessor. However, a proposed change of scoring system (setting the limit for KBD at 0.5 cm deviation from the straight line) would lower this deviation. We conclude that the developed automatic scoring technique is a reliable and potentially valuable tool for the assessment of KBD.
SARS-CoV-2 in Danish Mink Farms: Course of the Epidemic and a Descriptive Analysis of the Outbreaks in 2020
Authors: Boklund Anette , Hammer Anne Sofie , Quaade Michelle Lauge , Rasmussen Thomas Bruun , Lohse Louise , Strandbygaard Bertel , Jørgensen Charlotte Sværke , Olesen Ann Sofie , Hjerpe Freja Broe , Petersen Heidi Huus , Jensen Tim Kåre , Mortensen Sten , Calvo-Artavia Francisco F. , Lefèvre Stine Kjær , Nielsen Søren Saxmose , Halasa Tariq , Belsham Graham J. , Bøtner Anette
Published: January 2021

SARS-CoV-2 infection is the cause of COVID-19 in humans. In April 2020, SARS-CoV-2 infection in farmed mink (Neovision vision) occurred in the Netherlands. The first outbreaks in Denmark were detected in June 2020 in three farms. A steep increase in the number of infected farms occurred from September and onwards. Here, we describe prevalence data collected from 215 infected mink farms to characterize spread and impact of disease in infected farms. In one third of the farms, no clinical signs were observed. In farms with clinical signs, decreased feed intake, increased mortality and respiratory symptoms were most frequently observed, during a limited time period (median of 11 days). In 65% and 69% of farms, virus and sero-conversion, respectively, were detected in 100% of sampled animals at the first sampling. SARS-CoV-2 was detected, at low levels, in air samples collected close to the mink, on mink fur, on flies, on the foot of a seagull, and in gutter water, but not in feed. Some dogs and cats from infected farms tested positive for the virus. Chickens, rabbits, and horses sampled on a few farms, and wildlife sampled in the vicinity of the infected farms did not test positive for SARS-CoV-2. Thus, mink are highly susceptible to infection by SARS-CoV-2, but routes of transmission between farms, other than by direct human contact, are unclear.
Assessing Extensive Semi-Arid Rangeland Beef Cow–Calf Welfare in Namibia: Part 1: Comparison between Farm Production System’s Effect on the Welfare of Beef Cows
Authors: Kaurivi Yolande Baby , Laven Richard , Parkinson Tim , Hickson Rebecca , Stafford Kevin
Published: January 2021

A proposed animal welfare assessment protocol for semi-arid rangeland-based cow–calf systems in Namibia combined 40 measures from a protocol developed for beef cattle in New Zealand with additional Namibia-specific measures. Preliminary validation of the protocol had been undertaken with five herds in one semi-commercial village. The aim of the current study was to apply this protocol and compare animal welfare across three cow–calf production systems in Namibia. A total of 2529 beef cows were evaluated during pregnancy testing in the yards of 17 commercial, 20 semi-commercial, and 18 communal (total: 55) herds followed by an assessment of farm resources and a questionnaire-guided interview. Non-parametric tests were used to evaluate the difference in the welfare scores between the production systems. The results indicated a discrepancy of animal welfare between the three farm types, with a marked separation of commercial farms from semi-commercial, and communal village farms in the least. The differences in these production systems were mainly driven by economic gains through access to better beef export market for commercial farms and semi-commercial villages, as well as by the differences in the available grazing land, facility designs/quality, and traditional customs in the village systems. The results indicate an advantage of commercialization over communalization.
Factors Associated with the Introduction of Mycobacterium avium spp. Paratuberculosis (MAP) into Dairy Herds in Galicia (North-West Spain): The Perception of Experts
Authors: Villaamil Francisco Javier , Yus Eduardo , Benavides Bibiana , Allepuz Alberto , Moya Sebastián Jesús , Casal Jordi , Ortega Carmelo , Diéguez Francisco Javier
Published: January 2021

This study aimed at quantifying expert opinions on the risk factors involved in Mycobacterium avium subsp. paratuberculosis (MAP) infection in dairy cattle herds. For this purpose, potential risk factors associated with the introduction of MAP into dairies were chosen based on a literature review and discussions with researchers and veterinarians. For each factor, a decision tree was developed, and key questions were included in each. Answers to these key questions led to different events within each decision tree. An expert opinion workshop was organized (following the recommendations of the OIE), and ordinal values ranging from 0 to 9 (i.e., a null to very high likelihood of infection) were assigned to each event. The potential risk factors were also incorporated into a structured questionnaire that was responded to by 93 farms where the sanitary status against MAP was known. Thereby, based on the values given by the experts and the information collected in the questionnaires, each farm was assigned a score based on their MAP entry risk. From these scores (contrast variable) and using a ROC curve, the cut-off that best discriminated MAP-positive and -negative farms was estimated. The most important risk factors for the introduction of MAP, according to expert opinions, involved purchase and grazing practices related to animals under six months of age. The scores obtained for each farm, also based on the expert opinions, allowed MAP positive/MAP negative farms to be discriminated with 68.8% sensitivity and 68.7% specificity. These data should be useful for focusing future training initiatives and improving risk-reduction strategies in the dairy industry.
Anti-Coccidial Effect of Rumex Nervosus Leaf Powder on Broiler Chickens Infected with Eimeria Tenella Oocyst
Authors: Qaid Mohammed M. , Al-Mufarrej Saud I. , Azzam Mahmoud M. , Al-Garadi Maged A. , Albaadani Hani H. , Alhidary Ibrahim A. , Aljumaah Riyadh S.
Published: January 2021

Coccidiosis a huge economic burden in poultry farms where the pathogen Eimeria harms animal well-being and survival. Besides synthetic anti-coccidial drugs, natural herbs appear to be an alternative way to prevent avian coccidiosis. Rumex nervosus (RN), a phytogenic shrub, has received considerable attention in recent years due to its significant anti-microbial effects; however, limited knowledge exists about its potential anti-coccidial functions. This study was conducted to evaluate the prophylactic and therapeutic activities of RN leaf powder in broilers infected with Eimeria tenella. Infected chickens received a commercial diet containing 1, 3, or 5 g RN powder/kg diet compared to infected broilers that treated with Sacox (PC) or compared to uninfected broilers that received a commercial diet alone (NC). Results showed that RN powder significantly (p < 0.05) reduced the lesion scores and suppressed the output of oocysts per gram (OPG) in chickens’ feces. Although RN was unable to minimize the weight gain loss due to emeriosis, RN at level 1 g improved the feed conversion ratio. Therefore, RN powder, at 5 g, possesses moderate anti-coccidial effects and hence could be used to treat avian coccidiosis in field conditions; however, further studies are required to investigate, in vitro or in vivo, the anti-coccidial potential of active ingredients.
Potential Role of the Mitochondria for the Dermatological Treatment of Papillon-Lefèvre
Authors: Castejón-Vega Beatriz , Battino Maurizio , Quiles José L. , Bullon Beatriz , Cordero Mario D. , Bullón Pedro
Published: January 2021

The Papillon–Lefèvre syndrome (PLS) is a rare autosomal recessive disorder caused by mutations in the Cathepsin C (CTSC) gene, characterized by periodontitis and palmoplantar hyperkeratosis. The main inflammatory deficiencies include oxidative stress and autophagic dysfunction. Mitochondria are the main source of reactive oxygen species; their impaired function is related to skin diseases and periodontitis. The mitochondrial function has been evaluated in PLS and mitochondria have been targeted as a possible treatment for PLS. We show for the first time an important mitochondrial dysfunction associated with increased oxidative damage of mtDNA, reduced CoQ10 and mitochondrial mass and aberrant morphologies of the mitochondria in PLS patients. Mitochondrial dysfunction, determined by oxygen consumption rate (OCR) in PLS fibroblasts, was treated with CoQ10 supplementation, which determined an improvement in OCR and a remission of skin damage in a patient receiving a topical administration of a cream enriched with CoQ10 0.1%. We provide the first evidence of the role of mitochondrial dysfunction and CoQ10 deficiency in the pathophysiology of PLS and a future therapeutic option for PLS.
Visceral Adipose Tissue of Prediabetic and Diabetic Females Shares a Set of Similarly Upregulated microRNAs Functionally Annotated to Inflammation, Oxidative Stress and Insulin Signaling
Authors: Strycharz Justyna , Wróblewski Adam , Zieleniak Andrzej , Świderska Ewa , Matyjas Tomasz , Rucińska Monika , Pomorski Lech , Czarny Piotr , Szemraj Janusz , Drzewoski Józef , Śliwińska Agnieszka
Published: January 2021

Hypertrophic and hypoxic visceral adipose tissue (VAT) secretes proinflammatory cytokines promoting insulin resistance (IR), prediabetes and type 2 diabetes (T2DM) microRNAs (miRNAs) are markers of metabolic disorders regulating genes critical for e.g., inflammation, glucose metabolism, and antioxidant defense, with raising diagnostic value. The aim of the current study was to evaluate whether hyperglycemia is able to affect the expression of selected miRNAs in VAT of prediabetic (IFG) and diabetic (T2DM) patients vs. normoglycemic (NG) subjects using qPCR. Statistical analyses suggested that miRNAs expression could be sex-dependent. Thus, we determined 15 miRNAs as differentially expressed (DE) among NG, T2DM, IFG females (miR-10a-5p, let-7d-5p, miR-532-5p, miR-127-3p, miR-125b-5p, let-7a-5p, let-7e-5p, miR-199a-3p, miR-365a-3p, miR-99a-5p, miR-100-5p, miR-342-3p, miR-146b-5p, miR-204-5p, miR-409-3p). Majority of significantly changed miRNAs was similarly upregulated in VAT of female T2DM and IFG patients in comparison to NG subjects, positively correlated with FPG and HbA1c, yet, uncorrelated with WHR/BMI. Enrichment analyses indicated involvement of 11 top DE miRNAs in oxidative stress, inflammation and insulin signaling. Those miRNAs expression changes could be possibly associated with low-grade chronic inflammation and oxidative stress in VAT of hyperglycemic subjects.
Targeting Autophagy to Counteract Obesity-Associated Oxidative Stress
Authors: Pietrocola Federico , Bravo-San Pedro José Manuel
Published: January 2021

Reactive oxygen species (ROS) operate as key regulators of cellular homeostasis within a physiological range of concentrations, yet they turn into cytotoxic entities when their levels exceed a threshold limit. Accordingly, ROS are an important etiological cue for obesity, which in turn represents a major risk factor for multiple diseases, including diabetes, cardiovascular disorders, non-alcoholic fatty liver disease, and cancer. Therefore, the implementation of novel therapeutic strategies to improve the obese phenotype by targeting oxidative stress is of great interest for the scientific community. To this end, it is of high importance to shed light on the mechanisms through which cells curtail ROS production or limit their toxic effects, in order to harness them in anti-obesity therapy. In this review, we specifically discuss the role of autophagy in redox biology, focusing on its implication in the pathogenesis of obesity. Because autophagy is specifically triggered in response to redox imbalance as a quintessential cytoprotective mechanism, maneuvers based on the activation of autophagy hold promises of efficacy for the prevention and treatment of obesity and obesity-related morbidities.
Wire Electrical Discharge Machining, Mechanical and Tribological Performance of TiN Reinforced Multiscale SiAlON Ceramic Composites Fabricated by Spark Plasma Sintering
Authors: Grigoriev Sergey , Pristinskiy Yuri , Volosova Marina , Fedorov Sergey , Okunkova Anna , Peretyagin Pavel , Smirnov Anton
Published: January 2021

An effective approach for preparing electrically conductive multiscale SiAlON-based nanocomposites with 10 wt.% and 20 wt.% of titanium nitride was developed. Fully dense samples were obtained by spark plasma sintering (SPS) at 1700 °C and 80 MPa for 30 min. The morphology of nanocomposites was observed using scanning electron microscopy and the effects of TiN particles on the mechanical properties and electrical resistivity were studied. It was found that the addition of 20 wt.% TiN increased the hardness and fracture toughness compared to the commercial ceramic analogue TC3030. Meanwhile, the presence of TiN particles reduced the flexural strength of the nanocomposites due to the shrinkage difference between TiN particles and ceramic matrix during cooling, which led to tensile residual stresses and, consequently, to changes in strength values. In addition, the electrical resistivity of nanocomposites decreased with the increase of TiN content and reached 1.6 × 10−4 Ω∙m for 20 wt.% amount of second phase, which consequently made them suitable for electrical discharge machining. In addition to enhanced mechanical and electrical properties, under identical conditions, SPS-sintered multiscale nanocomposites exhibited a higher wear resistance (more than about 1.5-times) compared to the commercial sample due to their higher toughness and hardness.
Functional Traits of Plant Species Suitable for Revegetation of Landfill Waste from Nickel Smelter
Authors: Čierniková Malvína , Vykouková Ivana , Orfánus Tomáš , Masarovičová Elena
Published: January 2021

The landfill waste of leached ore residue represents a serious environmental risk and may also negatively affect the appearance, growth and development of vegetation. Here we focused on the evaluation of functional traits of selected plant species Populus alba, Calamagrostis epigejos, and Diplotaxis muralis growing in an unfavourable environment. We determined different adaptive strategies of selected species to extreme conditions. For Diplotaxis muralis the highest values of the leaf dry matter content (LDMC) and the lowest values of the specific leaf area (SLA) were determined, while for Calamagrostis epigejos these two traits correlated in opposite directions. Populus alba reached the lowest value of the water saturation deficit (WSD), suggesting that this species was most affected by soil water deficiency. The leaf water content (LWC) correlated negatively with the LDMC and positively with the SLA (narrow leaf blade). Although each plant species belongs to a different strategic group (therophyte, hemicryptophyte and phanerophyte in the juvenile stage), they are all very plastic and therefore suitable for remediation. Despite the unfavourable conditions, selected plant species were able to adapt to poor conditions and form more or less vital populations, which indicate the revegetation as a key measure for remediation of landfill waste from nickel smelter.
Mechanical Evaluation of Implant-Assisted Removable Partial Dentures in Kennedy Class I Patients: Finite Element Design Considerations
Authors: Messias Ana , Neto Maria A. , Amaro Ana M. , Lopes Vítor M. , Nicolau Pedro
Published: January 2021

The main purpose of this work was to construct a clinically valid numerical model of a mandibular Kennedy class I patient rehabilitated with a conventional removable partial denture and another two with implant-assisted removable partial dentures at two different implant locations. The selected patient was classified as ASA I and its mandible geometry reconstruction was performed by the conversion of the Cone-Beam computed Tomography (CBCT) scan raw medical data into a 3D model and subsequent conversion to a CAD file by reverse engineering methods. The soft tissue and removable denture geometries were also included in the CAD model as well as implants, ball attachments and matrix. Moreover, periodontal ligament was modelled by offsetting the mesh of the root surface of each tooth. The finite element results showed that the installation of a dental implant in each of the bilateral edentulous regions helps providing support and retention to the extension bases of the Removable Partial Denture (RPD) and significantly reduces the vertical and anterior-posterior displacements, regardless of its position.
One-Step Liquid Phase Polymerization of HEMA by Atmospheric-Pressure Plasma Discharges for Ti Dental Implants
Authors: Buxadera-Palomero Judit , Fricke Katja , Reuter Stephan , Gil Francisco Javier , Rodriguez Daniel , Canal Cristina
Published: January 2021

Dental implants can fail due to various factors, in which bad tissue integration is believed to have a significant role. Specific properties of the implant surface, such as its chemistry and roughness, are of paramount importance to address specific cell responses, such as the adsorption of proteins, as well as the adhesion and differentiation of cells, which are suitable for biomaterial and tissue engineering. In this study, an acrylate-containing coating was produced on titanium surfaces through the atmospheric pressure plasma treatment of a liquid precursor, 2-hydroxyethyl methacrylate. A hydrophilic coating was obtained, showing retention of the monomer chemistry as assessed by FTIR analysis and XPS. Enhanced fibroblast adhesion and decreased Staphylococcus aureus and Escherichia coli adhesion were recorded, showing that this is a suitable method to produce biocompatible coatings with a reduced bacterial adhesion.
Utilization of a Mobile Application for Motor Skill Evaluation in Children
Authors: Moreno Naya David , Vazquez-Araujo Francisco J. , Castro Paula M. , Vivas Costa Jamile , Dapena Adriana , González Doniz Luz
Published: January 2021

The detection of needs in educational reinforcement must be done in the early stages of childhood during primary education to improve the acquisition of motor skills. An adequate early intervention can solve some detected difficulties, thus contributing to children’s motor progress and mitigating possible deficiencies such as weak motor coordination. In this work, we present a mobile application (app) that allows physiotherapists to design activities for the evaluation of motor skills. These activities can be adapted to the child’s progress and the corresponding reinforcement needs. We tested the app with children from ages 6 to 12, corresponding to the six primary school grades. The results show that these children did not have notable motor difficulties, although children in lower primary school years showed a higher level of error in movement precision. The results allow us to establish reference values for a future comparison with collectives with motor difficulties.
Accelerating Detection of Lung Pathologies with Explainable Ultrasound Image Analysis
Authors: Born Jannis , Wiedemann Nina , Cossio Manuel , Buhre Charlotte , Brändle Gabriel , Leidermann Konstantin , Goulet Julie , Aujayeb Avinash , Moor Michael , Rieck Bastian , Borgwardt Karsten
Published: January 2021

Care during the COVID-19 pandemic hinges upon the existence of fast, safe, and highly sensitive diagnostic tools. Considering significant practical advantages of lung ultrasound (LUS) over other imaging techniques, but difficulties for doctors in pattern recognition, we aim to leverage machine learning toward guiding diagnosis from LUS. We release the largest publicly available LUS dataset for COVID-19 consisting of 202 videos from four classes (COVID-19, bacterial pneumonia, non-COVID-19 viral pneumonia and healthy controls). On this dataset, we perform an in-depth study of the value of deep learning methods for the differential diagnosis of lung pathologies. We propose a frame-based model that correctly distinguishes COVID-19 LUS videos from healthy and bacterial pneumonia data with a sensitivity of 0.90±0.08 and a specificity of 0.96±0.04. To investigate the utility of the proposed method, we employ interpretability methods for the spatio-temporal localization of pulmonary biomarkers, which are deemed useful for human-in-the-loop scenarios in a blinded study with medical experts. Aiming for robustness, we perform uncertainty estimation and demonstrate the model to recognize low-confidence situations which also improves performance. Lastly, we validated our model on an independent test dataset and report promising performance (sensitivity 0.806, specificity 0.962). The provided dataset facilitates the validation of related methodology in the community and the proposed framework might aid the development of a fast, accessible screening method for pulmonary diseases. Dataset and all code are publicly available at: https://github.com/BorgwardtLab/covid19_ultrasound.
Selected Aspects of Designing Modular PEMFC Stacks as Power Sources for Unmanned Aerial Vehicles
Authors: Dudek Magdalena , Lis Bartłomiej , Raźniak Andrzej , Krauz Mariusz , Kawalec Michał
Published: January 2021

Two types of air-cooled modular polymer membrane fuel cells (PEMFC) stacks with full equipment were constructed and investigated as components of hybrid power sources. The first, a 2-kW PEMFC stack, was assembled from two 1-kW PEMFC modules electrically connected in parallel and compared with a commercial PEMFC stack built from one 2-kW PEMFC module. The second, a 500-W PEMFC stack, was assembled with three modules connected in parallel. It was found that the two-module PEMFC stack was capable of operation with nominal power of 2 kW. Analysis of the distribution of the air cooling system in both modules was also conducted. The two-module PEMFC stack reduced hydrogen consumption compared to the reference 2-kW PEMFC stack consisting of only one module. The elaborated two-module PEMFC stack was successfully tested in a propulsion system designed to supply an electrical engine with a propeller. The electrical performance of the three-module PEMFC stack was tested separately as well as in a hybrid system in connection with a 5 s Li-Pol battery. It was found that the elaborated PEMFC stack was capable of operation with nominal power of 500 W and variable rapid dynamic electrical loads. It was also successfully tested as a power source to supply servomechanisms and other auxiliary devices.
Carbon from Bagasse Activated with Water Vapor and Its Adsorption Performance for Methylene Blue
Authors: Rahmawati Fitria , Ridassepri Arikasuci Fitonna , Chairunnisa  , Wijayanta Agung Tri , Nakabayashi Koji , Miyawaki Jin , Miyazaki Takahiko
Published: January 2021

This research work reports on the potential of bagasse, a solid waste from sugar factories, to produce activated-carbon (AC) as an adsorbent. The activation was conducted under 500, 600, and 700 °C using steam as the activation agent to produce AC500, AC600, and AC700, respectively. The prepared-materials were characterized to understand their elemental content, surface morphology, thermal properties, functional groups identification, surface area, and pore size. AC700 provided the highest surface area of 592.36 m2/g and indicated the contribution of mesopores distributes along 1.5–8.0 nm of pore size. Therefore, an adsorption test was conducted with AC700 as adsorbent. The results show that methylene blue (MB) adsorption reached equilibrium after 30 min of adsorption time. The adsorption isotherm applied to a monolayer Langmuir isotherm was fitted by linearization, resulting in a constant R2 of 0.999. The MB adsorption to AC700 favorably occurred, as proven by the Freundlich parameter 1/n of 0.881, which is less than 1. The Dubinin-Radushkevich isotherm confirmed that the adsorption proceeded through physical interaction with adsorption energy of 3.536 kJ/mol.
The Effect of Biopreparations and Biostimulants on the Chemical Composition and Microorganisms Associated with Verticillium Wilt of Horseradish Roots (Armoracia rusticana Gaertn.)
Authors: Gleń-Karolczyk Katarzyna , Boligłowa Elżbieta , Filipiak-Florkiewicz Agnieszka , Florkiewicz Adam , Luty Lidia
Published: January 2021

The basic foundation of plant production consists in plant protection treatments, which largely shape the quality of plant raw material intended for the food industry. Due to its long growing season, horseradish requires more treatments than average. Intensive protection based on synthetic pesticides favours the accumulation of their residues in plants and generates serious environmental problems. We have examined the effect of chemical protection and its reduced variant as well as three variants of biological protection, differentiated in terms of preparations used for seedlings treatment (Pythium oligandrum, Bacillus subtilis, and Ecklonia maxima), on the content of dry matter, protein, fat, carbohydrates, and the disease severity index (DSI) of Verticillium wilt of horseradish roots. The quantitative and qualitative changes in the microorganism communities associated with this disease were determined. Thirty-four species of fungi and Globisporangium irregulare have been shown to contribute to the pathogenesis of Verticillium wilt. The following were found with the highest frequency: Verticillium dahliae (14.37%), G. irregulare (6.87%), Ilyonectria destructans (6.73%), Fusarium acuminatum (6.53%), Rhisoctonia solani (6.75%), Epicoccum nigrum (5.56%), and Alternaria brassicae (5.36%).We found that water deficit promotes the increase of DSI and reduces the number and richness of fungal species isolated from diseased roots. The variant of biological protection with treatment of seedlings of E. maxima extract and with reduced chemical protectionin dry seasons give the best protective effects. Under conditions of optimal water supply, biological protection (P. oligandrum) is more effective than chemical protection. Biological protection increases biodiversity in the population of microorganisms that contribute to blackening of the conductive bundles of horseradish roots. There is a significant negative correlation between the number of isolated microorganisms and the DSI value. Increasing the number of the CFUs is accompanied by a reduction in the symptoms of Verticillium wilt of horseradish roots. Biological protection in years with sufficient water supply results in a significant increase in the content of dry matter, protein, and carbohydrates in horseradish roots. Under the influence of protective treatments, the ash content in the roots and its alkalinity decrease.
Thermochemical Heat Storage in a Lab-Scale Indirectly Operated CaO/Ca(OH)2 Reactor—Numerical Modeling and Model Validation through Inverse Parameter Estimation
Authors: Seitz Gabriele , Mohammadi Farid , Class Holger
Published: January 2021

Calcium oxide/Calcium hydroxide can be utilized as a reaction system for thermochemical heat storage. It features a high storage capacity, is cheap, and does not involve major environmental concerns. Operationally, different fixed-bed reactor concepts can be distinguished; direct reactor are characterized by gas flow through the reactive bulk material, while in indirect reactors, the heat-carrying gas flow is separated from the bulk material. This study puts a focus on the indirectly operated fixed-bed reactor setup. The fluxes of the reaction fluid and the heat-carrying flow are decoupled in order to overcome limitations due to heat conduction in the reactive bulk material. The fixed bed represents a porous medium where Darcy-type flow conditions can be assumed. Here, a numerical model for such a reactor concept is presented, which has been implemented in the software DuMux. An attempt to calibrate and validate it with experimental results from the literature is discussed in detail. This allows for the identification of a deficient insulation of the experimental setup. Accordingly, heat-loss mechanisms are included in the model. However, it can be shown that heat losses alone are not sufficient to explain the experimental results. It is evident that another effect plays a role here. Using Bayesian inference, this effect is identified as the reaction rate decreasing with progressing conversion of reactive material. The calibrated model reveals that more heat is lost over the reactor surface than transported in the heat transfer channel, which causes a considerable speed-up of the discharge reaction. An observed deceleration of the reaction rate at progressed conversion is attributed to the presence of agglomerates of the bulk material in the fixed bed. This retardation is represented phenomenologically by mofifying the reaction kinetics. After the calibration, the model is validated with a second set of experimental results. To speed up the calculations for the calibration, the numerical model is replaced by a surrogate model based on Polynomial Chaos Expansion and Principal Component Analysis.
Towards Integrated Digital Twins for Industrial Products: Case Study on an Overhead Crane
Authors: Autiosalo Juuso , Ala-Laurinaho Riku , Mattila Joel , Valtonen Miika , Peltoranta Valtteri , Tammi Kari
Published: January 2021

Industrial Internet of Things practitioners are adopting the concept of digital twins at an accelerating pace. The features of digital twins range from simulation and analysis to real-time sensor data and system integration. Implementation examples of modeling-oriented twins are becoming commonplace in academic literature, but information management-focused twins that combine multiple systems are scarce. This study presents, analyzes, and draws recommendations from building a multi-component digital twin as an industry-university collaboration project and related smaller works. The objective of the studied project was to create a prototype implementation of an industrial digital twin for an overhead crane called “Ilmatar”, serving machine designers and maintainers in their daily tasks. Additionally, related cases focus on enhancing operation. This paper describes two tools, three frameworks, and eight proof-of-concept prototypes related to digital twin development. The experiences show that good-quality Application Programming Interfaces (APIs) are significant enablers for the development of digital twins. Hence, we recommend that traditional industrial companies start building their API portfolios. The experiences in digital twin application development led to the discovery of a novel API-based business network framework that helps organize digital twin data supply chains.
Automated DNA Extraction Monitoring System Based on MTConnect Technology
Authors: Han Sang-Ho , Park Ae-Ja , Park Ah-Reum , Ryu Mun-Ho
Published: January 2021

MTConnect standard technology provides simplicity, flexibility, and scalability in integrating various equipment and operating systems and enabling accurate and consistent data collection from any MTConnect-compatible system. Using MTConnect technology, it is possible to immediately identify the cause of a problem and respond quickly when a problem occurs. Molecular genetic diagnostic point-of-care testing (POCT) devices have received attention in recent years because they enable rapid disease diagnosis. A molecular genetic diagnostic POCT device is under development by the authors. The system consists of a gene extraction process and a real-time PCR-based gene amplification process. In this study, we propose and demonstrate a system based on MTConnect technology to monitor an automated DNA extraction process. The proposed system consists of an automated DNA extraction system, an MTConnect adapter, an MTConnect agent, and a client application. The adapter and agent were developed on a Raspberry Pi single-board computer. The agent publishes the collected data in Extensible Markup Language (XML) format over a network. The performance and reliability of the system were evaluated by verifying the request response time between the implemented system’s agent and the client application. The results demonstrate the feasibility of monitoring the DNA extraction process over a network.
Internet of Nonthermal Food Processing Technologies (IoNTP): Food Industry 4.0 and Sustainability
Authors: Režek Jambrak Anet , Nutrizio Marinela , Djekić Ilija , Pleslić Sanda , Chemat Farid
Published: January 2021

With the introduction of Industry 4.0, and smart factories accordingly, there are new opportunities to implement elements of industry 4.0 in nonthermal processing. Moreover, with application of Internet of things (IoT), smart control of the process, big data optimization, as well as sustainable production and monitoring, there is a new era of Internet of nonthermal food processing technologies (IoNTP). Nonthermal technologies include high power ultrasound, pulsed electric fields, high voltage electrical discharge, high pressure processing, UV-LED, pulsed light, e-beam, and advanced thermal food processing techniques include microwave processing, ohmic heating and high-pressure homogenization. The aim of this review was to bring in front necessity to evaluate possibilities of implementing smart sensors, artificial intelligence (AI), big data, additive technologies with nonthermal technologies, with the possibility to create smart factories together with strong emphasis on sustainability. This paper brings an overview on digitalization, IoT, additive technologies (3D printing), cloud data storage and smart sensors including two SWOT analysis associated with IoNTPs and sustainability. It is of high importance to perform life cycle assessment (LCA), to quantify (En)—environmental dimension; (So)—social dimension and (Ec)—economic dimension. SWOT analysis showed: potential for energy saving during food processing; optimized overall environmental performance; lower manufacturing cost; development of eco-friendly products; higher level of health and safety during food processing and better work condition for workers. Nonthermal and advanced thermal technologies can be applied also as sustainable techniques working in line with the sustainable development goals (SDGs) and Agenda 2030 issued by United Nations (UN).
Can We Use Functional Annotation of Prokaryotic Taxa (FAPROTAX) to Assign the Ecological Functions of Soil Bacteria?
Authors: Sansupa Chakriya , Wahdan Sara Fareed Mohamed , Hossen Shakhawat , Disayathanoowat Terd , Wubet Tesfaye , Purahong Witoon
Published: January 2021

FAPROTAX is a promising tool for predicting ecological relevant functions of bacterial and archaeal taxa derived from 16S rRNA amplicon sequencing. The database was initially developed to predict the function of marine species using standard microbiological references. This study, however, has attempted to access the application of FAPROTAX in soil environments. We hypothesized that FAPROTAX was compatible with terrestrial ecosystems. The potential use of FAPROTAX to assign ecological functions of soil bacteria was investigated using meta-analysis and our newly designed experiments. Soil samples from two major terrestrial ecosystems, including agricultural land and forest, were collected. Bacterial taxonomy was analyzed using Illumina sequencing of the 16S rRNA gene and ecological functions of the soil bacteria were assigned by FAPROTAX. The presence of all functionally assigned OTUs (Operation Taxonomic Units) in soil were manually checked using peer-reviewed articles as well as standard microbiology books. Overall, we showed that sample source was not a predominant factor that limited the application of FAPROTAX, but poor taxonomic identification was. The proportion of assigned taxa between aquatic and non-aquatic ecosystems was not significantly different (p > 0.05). There were strong and significant correlations (σ = 0.90–0.95, p < 0.01) between the number of OTUs assigned to genus or order level and the number of functionally assigned OTUs. After manual verification, we found that more than 97% of the FAPROTAX assigned OTUs have previously been detected and potentially performed functions in agricultural and forest soils. We further provided information regarding taxa capable of N-fixation, P and K solubilization, which are three main important elements in soil systems and can be integrated with FAPROTAX to increase the proportion of functionally assigned OTUs. Consequently, we concluded that FAPROTAX can be used for a fast-functional screening or grouping of 16S derived bacterial data from terrestrial ecosystems and its performance could be enhanced through improving the taxonomic and functional reference databases.