bluuebunny/crossref_metadata_2025 huggingface dataset:
Nanomaterials and security in occupational and forensic medicine: insights from nanotoxicology
Authors: Tran Lang , Treglia Michele , Coppeta Luca , Ferrari Cristiana , Pallocci Margherita , Campagnolo Luisa , De Miranda Junior Luiz C. , Piccoli Bruno , Gaskin Sharyn , Cortes Fernandes Francisco , Dantas Filho Fabio , Passalacqua Pierluigi , Pietroiusti Antonio , Ippoliti Lorenzo , Bragaglia Mario , Nanni Francesca , Magrini Andrea , Marsella Luigi Tonino
Published: November 2024

Nanoenergetics are defined as a class of nanomaterials that possess the ability to release energy in certain situations. These properties have been studied and deepened in recent years, so much so that nanoenergetics have been introduced into the use of the weapons industry, among others. It is therefore an emerging reality that deserves attention with regard to potential harmful effects on human and environmental health. it has been suggested that nanoenergetics may have genotoxic and immunotoxic effects, among others. Problems related to exposure to nanonenergetics can therefore potentially affect both exposed workers (both in the production and use phase) and the civilian population, if used in war scenarios, for example,. Starting from these assumptions, the INNOTOX research project aims to contribute to the in-depth study of the toxicity of nanonenergetics, through an integrated approach involving experts in occupational and forensic medicine, nanotoxicology and bioengineering.
Detection of drug resistance in Escherichia coli from calves with diarrhea in the Tongliao region: an analysis of multidrug-resistant strains
Authors: Wang Zi , Sun Miao , Guo Shuang , Wang Yongqiang , Meng Linghao , Shi Jinchuan , Geng Chao , Han Dongxu , Fu Xiaomeng , Xue Jiangdong , Ma Hongxia , Liu Kai
Published: November 2024

Introduction

Escherichia coli is a major pathogen responsible for calf diarrhea, which has been exacerbated by the irrational and unscientific use of antimicrobial drugs, leading to significant drug resistance.

Methods

This study focused on the isolation and identification of E. coli from calf diarrhea samples in the Tongliao area of China. Isolation was conducted using selective media, Gram staining, and 16S rRNA sequencing. The minimum inhibitory concentration (MIC) of E. coli was determined through the microbroth dilution method. Additionally, the presence of antibiotic-resistant genes was detected, and multidrug-resistant strains were selected for whole-genome sequencing (WGS).

Results

The results revealed that all 40 isolated strains of E. coli exhibited resistance to sulfadiazine sodium, enrofloxacin, and ciprofloxacin, with 90% of the strains being susceptible to polymyxin B. Notably, strains 11, 23, and 24 demonstrated severe resistance. The detection rates of the antibiotic resistance genes TEM-1, TEM-206, strA, strB, qacH, and blaCTX were 100%, indicating a high prevalence of these genes. Moreover, the majority of strains carried antibiotic resistance genes consistent with their resistance phenotypes. WGS of strains 11, 23, and 24 revealed genome sizes of 4,897,185 bp, 4,920,234 bp, and 4,912,320 bp, respectively. These strains carried two, one, and two plasmids, respectively. The prediction of antibiotic resistance genes showed a substantial number of these genes within the genomes, with strain 24 harboring the highest number, totaling 77 subspecies containing 88 antibiotic resistance genes.

Discussion

In conclusion, all 40 isolated strains of E. coli from calf diarrhea in this study were multidrug-resistant, exhibiting a broad distribution of antibiotic resistance genes and mobile components. This poses a significant risk of horizontal gene transfer, highlighting the critical situation of antibiotic resistance in this region.
Specific detection of duck adeno-associated virus using a TaqMan-based real-time PCR assay
Authors: Chen Shuyu , Chen YuYi , Zhang Mengyan , Zhang Wenyu , Fu Huanru , Huang Yu , Cheng Longfei , Wan Chunhe
Published: November 2024

Duck adeno-associated Virus (DAAV) is a novel pathogen that was recently discovered in ducks. To establish a molecular detection assay for DAAV for further epidemiological investigation and pathogenic mechanism. Here, we designed specific primers and probes according to the sequence characteristics of the newly discovered DAAV and then established a TaqMan real-time PCR method (TaqMan-qPCR) for the detection of DAAV. Our data showed that the established TaqMan-qPCR for detecting DAAV had high sensitivity, with the lowest detection limit of 29.1 copies/μL. No cross reaction was found with duck circovirus (DuCV), H9N2 subtype avian influenza virus (AIV), avian Tembusu virus (ATmV). duck hepatitis A virus 1 and 3 (DHAV-1 and DHAV-3), duck adenovirus A (DAdV-A), duck adenovirus 3 (DAdV-3), or duck enteritis virus (DEV). The repeatability was excellent, with the coefficients of variation of repeated intragroup and intergroup tests ranging from 0.12–0.21% and 0.62–1.42%, respectively. Seventy-eight clinical samples collected from diseased or deceased ducklings were tested. The results showed that the DAAV positive rate was 21.79%, and a triple infection (DAAV+MDPV+GPV) was found. These data provide technical support for further molecular epidemiological surveillance and pathogenic mechanism studies of DAAV infection.
A subunit vaccine based on P97R1, P46, P42, and P65 from Mycoplasma hyopneumoniae can induce significant immune response in piglets
Authors: He Yintao , Xie Kaiyuan , Yuan Zhongmao , Ouyang Ting , Dong Anran , Ling Bing , Zeng Weijun , Fang Yiqi , Song Yiwan , Wang Lianxiang , Ding Hongxing , Zhao Mingqiu , Fan Shuangqi , Yi Lin , Zhao Dongfang , Chen Jinding
Published: November 2024

Mycoplasma pneumonia (MPS), caused by Mycoplasma hyopneumoniae (Mhp), is a chronic, airborne respiratory disease that poses a significant threat to the global swine industry. The P97 and P46 proteins are major antigens of Mhp, with the R1 region of P97 possessing full adhesive capability. Studies have shown that the main antigenic regions of Mhp P42 and P65 proteins exhibit strong immunogenicity. In this study, we first linked the genes encoding P97R1 and P46 proteins to form the P97R1P65 gene and subsequently constructed three shuttle plasmids: pFBD-P97R1P46, pFBD-P97R1P46-p65, and pFBD-P65-P42. These proteins were expressed using the Bac to Bac system and formulated into subunit vaccines for mouse immunization. Mouse experiments indicated that the P97R1P46 + P65-P42 protein combination elicited higher levels of specific antibodies, IL-2, IL-4, and CD8+ T cells compared to other subunit vaccine groups, a finding further validated in subsequent mouse challenge protection experiments. Therefore, we utilized the MultiBac expression system to co-express P97R1P46, P65, and P42 proteins in the pFastMultibacDual vector for immunization experiments in piglets. The piglet immunization experiments demonstrated that the Mhp subunit vaccine prepared in this study could induce specific antibodies against Mhp, with the combination of P97R1P46, P65, and P42 proteins inducing the highest level of humoral immunity. This study provides valuable insights for the development of Mhp subunit vaccines.
Quotient Network-A Network Similar to ResNet but Learning Quotients
Authors: Hui Peng , Zhao Jiamuyang , Li Changxin , Zhu Qingzhen
Published: November 2024

The emergence of ResNet provides a powerful tool for training extremely deep networks. The core idea behind it is to change the learning goals of the network. It no longer learns new features from scratch but learns the difference between the target and existing features. However, the difference between the two kinds of features does not have an independent and clear meaning, and the amount of learning is based on the absolute rather than the relative difference, which is sensitive to the size of existing features. We propose a new network that perfectly solves these two problems while still having the advantages of ResNet. Specifically, it chooses to learn the quotient of the target features with the existing features, so we call it the quotient network. In order to enable this network to learn successfully and achieve higher performance, we propose some design rules for this network so that it can be trained efficiently and achieve better performance than ResNet. Experiments on the CIFAR10, CIFAR100, and SVHN datasets prove that this network can stably achieve considerable improvements over ResNet by simply making tiny corresponding changes to the original ResNet network without adding new parameters.
Shake It Off: Investigating the Function of a Domestic Dog Behavior in Social Contexts
Authors: Bryce Ani , Nurkin Paige , Horowitz Alexandra
Published: November 2024

Shaking in domestic dogs—a rapid side-to-side movement of the body or head—is a commonly observed behavior, yet its function remains minimally researched. The present study aimed to investigate the use of shaking behavior in naturalistic social contexts, with the hypothesis that shaking functions as a marker of transition between behaviors or activities. In addition, as the prior literature has suggested that shaking more frequently occurred in postures or conditions of stress, either to signal stress or to mitigate it, we looked at postures related to affect before and after shaking. We observed 96 New York City dogs in two contexts: a public dog run and a private daycare. Over 120 episodes, shaking behavior was most often observed between two different activities or behavioral categories, supporting the hypothesis that shaking functions as a marker of transition between behaviors and activities. We also found neither a significant change in postures related to affect nor in the number of dogs in the subject’s vicinity after shaking. While there may be other functions of canine shaking behavior, this observational research adds to our understanding of a common and understudied dog behavior.
The Bioavailability of Solid-State Fermented Feather Meal Using a Novel Feather-Degrading Bacterium Bacillus velezensis PN1 in Broilers
Authors: Kuo Tung-Lung , Wei Hen-Wei
Published: November 2024

In this study, an effective feather-degrading bacterium was isolated and the solid-state fermentation condition for feather degradation was optimized. The resulting fermented feather meal (FFM) was evaluated for its bioavailability in broilers. Four Bacillus strains were examined for feather degradation rates, with Bacillus velezensis PN1 exhibiting the highest rate (83.24%, p < 0.05). A 3 × 3 × 3 factorial design was used to test substrate moisture content (45%, 55%, 65%), temperature (27 °C, 37 °C, 47 °C), and incubation time (24, 48, 72 h) for optimized fermentation conditions. In vitro pepsin digestibility (IVPD) revealed a significant interaction between temperature and time (p < 0.05), and the optimal performance was achieved at 37 °C for 72 h, followed by 37 °C for 48 h. Considering production time and cost, FFM2 (produced with 65% moisture at 37 °C for 48 h) was further compared with FFM1 (produced under the same conditions but at a lower temperature of 27 °C), and commercial hydrolyzed feather meal (HFM). IVPD did not differ significantly between FFM1, FFM2, and HFM, as they all showed significantly higher digestibility compared to raw feathers (RFs). FFM1, as well as FFM2, had significantly higher histidine and lysine concentrations than RF (p < 0.05), while FFM2 had a significantly lower cysteine content (p < 0.05). Based on fermentation conditions and amino acid composition, FFM2 was considered more suitable for large-scale production and was used in a broiler growth trial. The inclusion of 5% FFM2 in the broiler diet did not significantly affect body weight at 35 days compared to the 5% HFM group (p > 0.05), although both groups showed significantly lower weight gain than the 5% fish meal (FM) group (p < 0.05). The feed conversion ratio and performance efficiency factor did not differ significantly between the FFM2, HFM, and FM groups (p > 0.05). In groups fed diets without supplemental crystalline amino acids, growth performance did not significantly differ between the HFM and FFM2 groups (p > 0.05), although both performed significantly worse than groups with amino acid supplementation. In conclusion, FFM produced by B. velezensis PN1 through solid-state fermentation enhances feather bioavailability in poultry and can completely replace HFM when included at 5% in broiler diets, offering a potential sustainable alternative for poultry nutrition on a commercial scale.
CRISPR-Cas13a-Based Lateral Flow Assay for Detection of Bovine Leukemia Virus
Authors: Zhao Yuxi , Dai Jingwen , Zhang Zhen , Chen Jianguo , Chen Yingyu , Hu Changmin , Chen Xi , Guo Aizhen
Published: November 2024

Bovine leukemia virus (BLV) is the causative agent of enzootic bovine leukosis (EBL), which presents worldwide prevalence. BLV caused substantial economic loss in China around the 1980s; then, it could not be detected for some time, until recently. Due to its latent and chronic characteristics, the efficient and accurate detection of BLV is of utmost significance to the timely implementation of control measures. Therefore, this study harnessed the recombinase-aided amplification (RAA), clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein 13a (Cas13a) technology, and lateral flow (LF) strips to develop an efficient method for detection of BLV. In this method, isothermal amplification of the targeted pol gene is performed at 37 °C with a detection threshold of 1 copy/µL, and the procedure is completed in 100 min. This assay demonstrated high selectivity for BLV, as indicated by the absence of a cross-reaction with six common bovine pathogens. Remarkably, 100 blood samples from dairy cows were tested in parallel with a conventional quantitative polymerase chain reaction (qPCR) and this method and the results showed 100% agreement. Furthermore, this method exhibited good repeatability. In conclusion, in this study, we established a sensitive and specific method for BLV detection, which shows promise for application in BLV surveillance.
Single-Nucleus RNA Sequencing Reveals the Transcriptome Profiling of Ovarian Cells in Adolescent Cyprinus carpio
Authors: Hou Mingxi , Zhang Jin , Wang Qi , Zhao Ran , Cao Yiming , Chen Yingjie , Wang Kaikuo , Ding Ning , Qi Yingjie , Sun Xiaoqing , Zhang Yan , Li Jiongtang
Published: November 2024

The common carp (Cyprinus carpio) is a crucial freshwater species cultivated worldwide for food consumption. Female carp have better growth performance than males, which fascinates scholars to uncover the mechanism of gonadal differentiation and produce mono-sex populations. However, the mechanism of ovarian development at single-cell resolution is limited. Here, we conducted single-nucleus RNA sequencing in adolescent common carp ovaries. Our study obtained transcriptional profiles of 13,155 nuclei and revealed 13 distinct cell clusters in the ovaries, including three subtypes of germ cells and four subtypes of granulosa cells. We subsequently performed pseudotime trajectory analysis to delineate potential mechanisms underlying the development of germ cells and granulosa cells. We identified 1250 dynamic expression genes in germ cells and 1815 in granulosa cells (q-value < 0.01), including zp3, eif4a2 and aspm in germ cells and fshr and esr1 in granulosa cells. The functional annotation showed that dynamic expression genes in germ cells were involved in sperm–egg recognition and some terms related to meiosis, such as sister chromatid segregation and homologous recombination. Genes expressed dynamically in granulosa cells were related to the TGF-β signaling pathway, response to gonadotropin, and development of primary female sexual characteristics. In addition, the dynamic genes expressed in granulosa cells might relate to the complex communication between different cell types. In summary, our study provided a transcriptome profile of common carp ovaries at single-nucleus resolution, and we further revealed the potential cell type-specific mechanisms underlying oogenesis and the differentiation of granulosa cells, which will facilitate breeding all-female common carp populations.
Mitigating the Effects of Maternal Loss on Harbour Seal Pups in Captive Care
Authors: Wilson Susan , Alger Rhiannon
Published: November 2024

Stranded newborn “orphan” harbour seal pups entering captive care are often maintained for some weeks in isolation, mainly as a precaution against the spread of infection. However, this practice raises concerns for the welfare and normal socialisation of pups, who normally spend their first post-natal weeks close to their mothers and other seals. The present study recorded and described the behaviour of six paired orphan pups in rehabilitation up to about five weeks of age, provided with free access to water and haul-out areas. The occurrences of resting, following, nosing and body contacts, and aquatic play were recorded and compared qualitatively and quantitatively with the same behaviours of free-living pups with their mothers. The pups entered the water every day, although more often from about 2.5 weeks of age. They displayed to each other the same behaviours that free-living pups display to their mothers, although they engaged in relatively more physical contact, body nosing, and aquatic play. The study has shown that orphan pups maintained in pairs with free water access can act reciprocally as mother substitutes, thereby promoting species-typical primary socialisation and welfare during their early days of captive care.
Characterization and Potential Application of Phage vB_PmuM_CFP3 for Phage Therapy Against Avian Pasteurella multocida
Authors: Chen Hongmei , Jiang Nansong , Fu Guanghua , Fu Qiuling , Wan Chunhe , Huang Yu , Liu Yuan , Liu Rongchang , Liang Qizhang , Cheng Longfei
Published: November 2024

The rise of antibiotic-resistant bacterial infections necessitates alternative therapeutic strategies, such as phage therapy. This study investigates the potential of phage vB_PmuM_CFP3 (CFP3) as a therapeutic agent against avian cholera caused by Pasteurella multocida (P. multocida). Phage CFP3 was isolated from the feces and wastewater of a laying hen farm and underwent comprehensive biological characterization, including host range, lytic activity, and environmental stability. Transmission electron microscopy revealed CFP3′s typical myovirus morphology, with a head diameter of approximately 60 nm and a tail length of about 120 nm. CFP3 demonstrated high stability across a pH range of 4–10 and temperatures of 30–40 °C, making it suitable for oral administration in poultry. The phage exhibited a latent period of about 90 min and an optimal multiplicity of infection (MOI) of 1. Despite its narrow host range, with a lysis rate of 28.2% against avian-derived type A P. multocida, CFP3′s specificity minimizes impact on non-target bacteria. Whole-genome sequencing revealed a 32,696 bp linear double-stranded DNA genome with 46 predicted open reading frames (ORFs) and no tRNA or antibiotic resistance genes, enhancing its safety profile. Phylogenetic analysis indicated a close evolutionary relationship with Haemophilus phages HP1, HP2, and Pasteurella phage F108. While CFP3 shows promise as a precision therapeutic tool, further in vivo studies are required to evaluate its efficacy and safety. Future research should focus on expanding the phage library, optimizing phage mixtures, and exploring synergistic effects with other antimicrobial strategies. This study provides foundational data supporting the development of CFP3 as a viable alternative to antibiotics for controlling avian cholera.
Correction: Olaguer et al. Landfill Emissions of Methane Inferred from Unmanned Aerial Vehicle and Mobile Ground Measurements. Atmosphere 2022, 13, 983
Authors: Olaguer Eduardo P. , Jeltema Shelley , Gauthier Thomas , Jermalowicz Dustin , Ostaszewski Arthur , Batterman Stuart , Xia Tian , Raneses Julia , Kovalchick Michael , Miller Scott , Acevedo Jorge , Lamb Jonathan , Benya Jeff , Wendling April , Zhu Joyce
Published: November 2024

There was an error in the original publication [...]
Role of the Europe–China Pattern Teleconnection in the Interdecadal Autumn Dry–Wet Fluctuations in Central China
Authors: Jiang Linwei , Gao Wenhao , Zhu Kexu , Zheng Jianqiu , Ren Baohua
Published: November 2024

Based on statistical analyses of long-term reanalysis data, we have investigated the interdecadal variations of autumn precipitation in central China (APC-d) and the associated atmospheric teleconnection. It reveals that the increased autumn rainfall in central China during the last decade is a portion of the APC-d, which exhibits a high correlation coefficient of 0.7 with the interdecadal variations of the Europe–China pattern (EC-d pattern) teleconnection. The EC-d pattern teleconnection presents in a “+-+” structure over Eurasia, putting central China into the periphery of a quasi-barotropic anticyclonic high-pressure anomaly. Driven by positive vorticity advection and the inflow of warmer and moist air from the south, central China experiences enhanced ascending motion and abundant water vapor supply, resulting in increased rainfall. Further analysis suggests that the EC-d pattern originates from the exit of the North Atlantic jet and propagates eastward. It is captured by the Asian westerly jet stream and proceeds towards East Asia through the wave–mean flow interaction. The wave train acquires effective potential energy from the mean flow by the baroclinic energy conversion and simultaneously obtains kinetic energy from the basic westerly jet zones across the North Atlantic and the East Asian coasts. The interdecadal variation of the mid-latitude North Atlantic sea surface temperature (MAT-d) exhibits a significant negative relationship with EC-d, serving as a modulating factor for the EC-d pattern teleconnection. Experiments with CMIP6 models predict that the interdecadal variations in APC-d, EC-d, and MAT-d will maintain stable high correlations for the rest of the 21st century. These findings may contribute to forecasting the interdecadal autumn dry–wet conditions in central China.
The Stability of a Predator–Prey Model with Cross-Dispersal in a Multi-Patch Environment
Authors: Xu Keyao , Peng Keyu , Gao Shang
Published: November 2024

This paper investigates the stability of predator–prey models within multi-patch environments, with a particular focus on the influence of cross-dispersion across patches. We apply Kirchhoff’s matrix tree theorem and Liapunov’s method to derive criteria related to the cross-dispersion topology, thus solving the challenge of determining global asymptotic stability conditions. The method incorporates realistic ecological interactions and spatial heterogeneity, offering a framework for stability analysis. Our findings demonstrate that an appropriate level of cross-dispersion can effectively mitigate oscillations and foster convergence toward equilibrium. Two numerical examples validate these theoretical results and demonstrate the feasibility and effectiveness of the model across multiple patches.
Innovative Peptide-Based Plasmonic Optical Biosensor for the Determination of Cholesterol
Authors: Bernardo Ana Lia , Parra Anne , Cebrián Virginia , Ahumada Óscar , Oddi Sergio , Dainese Enrico
Published: November 2024

Plasmonic-based biosensors have gained prominence as potent optical biosensing platforms in both scientific and medical research, attributable to their enhanced sensitivity and precision in detecting biomolecular and chemical interactions. However, the detection of low molecular weight analytes with high sensitivity and specificity remains a complex and unresolved issue, posing significant limitations for the advancement of clinical diagnostic tools and medical device technologies. Notably, abnormal cholesterol levels are a well-established indicator of various pathological conditions; yet, the quantitative detection of the free form of cholesterol is complicated by its small molecular size, pronounced hydrophobicity, and the necessity for mediator molecules to achieve efficient sensing. In the present study, a novel strategy for cholesterol quantification was developed, leveraging a plasmonic optical readout in conjunction with a highly specific cholesterol-binding peptide (C-pept) as a biorecognition element, anchored on a functionalized silica substrate. The resulting biosensor exhibited an exceptionally low detection limit of 21.95 µM and demonstrated a linear response in the 10–200 µM range. This peptide-integrated plasmonic sensor introduces a novel one-step competitive method for cholesterol quantification, positioning itself as a highly sensitive biosensing modality for implementation within the AVAC platform, which operates using reflective dark-field microscopy.
Association Between the Enriched Environment Level and Serum Brain-Derived Neurotrophic Factor (BDNF) in Patients with Major Depressive Disorder
Authors: Vega-Rosas Andrés , Flores-Ramos Mónica , Ramírez-Rodríguez Gerardo Bernabé
Published: November 2024

Major Depressive Disorder (MDD) is a neuropsychiatric condition whose neurobiological characteristics include alterations in brain plasticity, modulated by Brain-Derived Neurotrophic Factor (BDNF). In animal models, environmental enrichment promotes neuroplasticity and reduces depressive-like behaviors. In humans, we proposed to assess the level of Enriched Environment (EE) using a questionnaire that includes different domains of the EE (cognitive, social, and physical), which we named the EE Indicator (EEI). Objective: To determine the relationship between the level of EE and serum BDNF in participants with MDD and healthy controls. Materials: Participants with MDD without antidepressant treatment and healthy controls were recruited, and their EE level and serum BDNF concentration were determined looking for correlations between their clinical characteristics and the cognitive, social, and physical activities according to the EEI. Results: A total of 25 participants were recruited, of which 6 participants with MDD and the same number of controls were selected in a paired manner. Although no differences were found in the concentration of BDNF between the groups, positive correlations were observed between cognitive EE and BDNF (r = 0.62, p = 0.035), as well as negative social EE and the Hamilton Depression Rating Scale (HDRS) (r = −0.86, p = 0.001). The sum between cognitive and social EE showed a positive correlation with the serum concentration of BDNF (r = 0.34, p = 0.0451). Conclusions: The level of EE is potentially modulating the presence and severity of MDD at a clinical level, but it can also influence at a neuroplastic level through promoting or limiting the concentration of BDNF.
Efficacy and Safety of Lithium for Suicide and Suicide-Related Behaviors in Youth: A Review of the Literature
Authors: Sesso Gianluca , Bargnesi Francesca , Olzi Francesca , Mutti Giulia , Berloffa Stefano , Viglione Valentina , Fantozzi Pamela , Tolomei Greta , Guccione Fulvio , Milone Annarita , Masi Gabriele
Published: November 2024

Objectives: This systematic review evaluates the anti-suicidal properties of Lithium in children and adolescents with Bipolar Disorder (BD), addressing gaps in evidence regarding its efficacy and safety in reducing suicidality and self-harming behaviors. Methods: A comprehensive literature search was conducted across PubMed, Web of Science, and Scopus up to February 2024. Eligible studies were those focusing on patients aged 25 years or younger, examining Lithium therapy and its impact on suicidal ideation and behaviors. The review included randomized controlled trials, longitudinal prospective and retrospective studies, and cross-sectional studies, while excluding expert opinions and case reports. Results: Evidence generally supports the efficacy of Lithium in reducing suicidal ideation and self-harming behaviors in youth with BD, though results are mixed. Randomized controlled trials demonstrated its effectiveness in mitigating suicidal thoughts during acute manic episodes, with effects persisting post-treatment. Longitudinal studies suggested that Lithium might offer superior outcomes compared to other mood stabilizers, although its specific impact on suicidality remains inconclusive. Cross-sectional studies and retrospective analyses reveal associations between Lithium use and reduced self-harming behaviors, but causality remains uncertain. While mood-stabilizing effects of Lithium offer potential benefits for reducing suicidality in youth, evidence on its direct impact on emotional dysregulation (ED) and long-term efficacy is limited. Variability in individual responses and adherence issues underscore the need for further research. Future studies should include larger, diverse samples, focus on ED symptoms, and explore Lithium mechanisms in suicidality prevention. Conclusions: Lithium remains a promising treatment for mood stabilization and reduction in suicidality in youth with BD.
Leadership Development in Women STEM Students: The Interplay of Task Behaviors, Self-Efficacy, and University Training
Authors: Coluccio Giuliani , Muñoz-Herrera Sebastián , Adriasola Elisa , Escobar Elizabeth
Published: November 2024

This study explores the relationship between task-oriented behaviors, self-efficacy, and leadership emergence in women STEM students, grounded in the context of prototypical leadership theory and self-efficacy theory. Prototypical leadership theory emphasizes the alignment of leadership behaviors with group expectations, which, in STEM fields, are often task-oriented. The research examines how task-oriented behaviors, such as planning, decision-making, and supervision, influence women’s self-perception of leadership ability and their subsequent emergence as leaders. Our results show a positive relationship between task-oriented behaviors and self-efficacy and a positive relationship between self-efficacy with leader emergence, with academic experience further ngthening this link. As students’ progress through their programs, engaging in more teamwork and leadership tasks, their self-efficacy enhances, leading to stronger leadership emergence. Also, we found an indirect effect from task-oriented behavior to leader emergence via self-efficacy. These findings have significant implications for fostering leadership in women, particularly in STEM. The study calls for educational programs to enhance opportunities for women to develop these behaviors early on, ensuring their growth into leadership roles in STEM fields.
Microbial-Induced Calcium Carbonate Precipitation and Basalt Fiber Cloth Reinforcement Used for Sustainable Repair of Tunnel Lining Cracks
Authors: Wan Siqi , Shu Zhile , Kang Shun , Zhong Wenwu , Zhang Xin , Wu Haikuan , Liu Ruizhi
Published: November 2024

The increasing problem of urban traffic congestion has led to the extensive use of underground tunnels. However, tunnel lining cracks pose a major threat to the integrity and safety of the structure. Although the traditional repair method is effective, it often requires higher construction technology and higher cost, and may cause damage to the concrete structure. In this study, microbial-induced calcium carbonate precipitation (MICP) was combined with basalt fiber cloth to repair and reinforce tunnel lining cracks. Bacillus pasteurii was used to optimize the microbial mineralization process, and the effectiveness of the method on cracks with different widths was evaluated using a water seepage test. In addition, the mechanical properties of the reinforced tunnel lining were tested. The microbial mineralization process effectively repaired cracks with widths of 1 mm, 2 mm, and 3 mm. The use of unidirectional basalt fiber cloth increased the bearing capacity of the strengthened member by 12.5%. The combined reinforcement method also enhances the deflection performance and alleviates the influence of water seepage on the bonding performance. This innovative and sustainable approach not only provides an effective solution for the repair of tunnel lining cracks, but also contributes to the broader field of eco-friendly building materials. This study highlights the potential of using this combination approach to improve the durability and performance of underground infrastructure.
Workability of Nanomodified Self-Compacting Geopolymer Concrete Based on Response Surface Method
Authors: Tian Yong-Hua , Tao Jia-Cheng , Luo Tao , Li Li
Published: November 2024

Geopolymer concrete is more low-carbon and environmentally friendly than Portland cement concrete. Nanoparticle modification can help to improve the mechanical and durability performance of concrete, but due to its large specific surface area and high activity, it may deteriorate its workability. However, there is currently limited research on the effect of nanomodification on the workability of freshly mixed self-compacting geopolymer concrete (SCGC). This article conducted SCGC workability experiments using the response surface methodology, which included 29 different mixtures. The effects of nano-silica (NS), nano-calcium carbonate (NC), alkali content (N/B), and water cement ratio (W/B) on the workability of SCGC were studied. The experimental results show that the addition of NS and NC can reduce the slump expansion of SCGC, and the combination of the two significantly increases the amplitude of slump expansion with the change in nanomaterial content. An increase in N/B will reduce the expansion time and clearance value of SCGC. As N/B increases from 4% to 4.4%, the slump extension of SCGC decreases, and with a further increase in N/B, the slump extension increases significantly to 68.1 cm, which means that the slump extension of SCGC increases by 9.5% as N/B increases from 4.4 to 5. This study can provide a reference for optimizing the fresh performance of geopolymer concrete and improving the mechanism of nanomaterial-modified geopolymer concrete.
Harnessing Bacterial Agents to Modulate the Tumor Microenvironment and Enhance Cancer Immunotherapy
Authors: Thomas Christina James , Delgado Kaylee , Sawant Kamlesh , Roy Jacob , Gupta Udit , Song Carly Shaw , Poojary Rayansh , de Figueiredo Paul , Song Jianxun
Published: November 2024

Cancer immunotherapy has revolutionized cancer treatment by leveraging the immune system to attack tumors. However, its effectiveness is often hindered by the immunosuppressive tumor microenvironment (TME), where a complex interplay of tumor, stromal, and immune cells undermines antitumor responses and allows tumors to evade immune detection. This review explores innovative strategies to modify the TME and enhance immunotherapy outcomes, focusing on the therapeutic potential of engineered bacteria. These bacteria exploit the unique characteristics of the TME, such as abnormal vasculature and immune suppression, to selectively accumulate in tumors. Genetically modified bacteria can deliver therapeutic agents, including immune checkpoint inhibitors and cytokines, directly to tumor sites. This review highlights how bacterial therapeutics can target critical immune cells within the TME, such as myeloid-derived suppressor cells and tumor-associated macrophages, thereby promoting antitumor immunity. The combination of bacterial therapies with immune checkpoint inhibitors or adoptive cell transfer presents a promising strategy to counteract immune suppression. Continued research in this area could position bacterial agents as a powerful new modality to reshape the TME and enhance the efficacy of cancer immunotherapy, particularly for tumors resistant to conventional treatments.
Differences in Outcomes Based on the Degree to Which Bone Defects Are Filled with Cancellous Allochip Bone Grafts in Hand Enchondroma Patients
Authors: Kang Sung Ju , Lim Jun-Hyuk , Kim Chan Wi , Kang Gyo Rim , Kim Sungmin , Jung Sung-Taek
Published: November 2024

Background/Objectives: Enchondroma, the most common benign cartilage tumor in the hand, often presents as pain, swelling, or pathological fractures. While curettage is the standard treatment preventing fractures, the optimal way of managing the bone cavity remains debated. In this study, we investigated the impact of a filled bone defect on radiologic and clinical outcomes among patients with enchondroma. Methods: We retrospectively reviewed patients with solitary enchondroma of the hand who underwent curettage followed by allogeneic cancellous bone chip impaction grafting. The patients were divided into two groups based on the extent to which their bone defects were filled post-curettage: Group 1 (complete filling) and Group 2 (incomplete filling, i.e., <50%). We reviewed demographic data, local recurrence data, complications, information on consolidation duration, data on range of motion (ROM), and functional scores. Results: This study included 59 patients (25 males and 34 females; mean age, 30.4 ± 11.9 years, with a range of 8–78). Group 1 contained 35 patients, and Group 2 contained 24. No nonunion occurred following curettage and grafting. The mean radiological consolidation period was 6.4 weeks (range: 5–18). There was no significant difference in consolidation time between Group 1 (6.8 weeks) and Group 2 (6.9 weeks) (p = 0.166). The ROM and functional scores also showed no significant differences between the groups, with musculoskeletal society scores of 98.8 for Group 1 and 99.8 for Group 2 (p = 0.63). Conclusions: This study demonstrates that the use of the impaction technique combined with cancellous allochip bone grafting yields favorable results in the treatment of solitary hand enchondroma. The extent to which the bone defect was filled did not significantly impact the overall outcomes.
Longitudinal FDG-PET Radiomics for Early Prediction of Treatment Response to Chemoradiation in Locally Advanced Cervical Cancer: A Pilot Study
Authors: Cepero Alejandro , Yang Yidong , Young Lori , Huang Jianfeng , Ji Xuemei , Yang Fei
Published: November 2024

Objectives: This study aimed to assess the capacity of longitudinal FDG-PET radiomics for early distinguishing between locally advanced cervical cancer (LACC) patients who responded to treatment and those who did not. Methods: FDG-PET scans were obtained before and midway through concurrent chemoradiation for a study cohort of patients with LACC. Radiomics features related to image textures were extracted from the primary tumor volumes and stratified for relevance to treatment response status with the aid of random forest recursive feature elimination. Predictive models based on the k-nearest neighbors time series classifier were developed using the top-selected features to differentiate between responders and non-responders. The performance of the developed models was evaluated using receiver operating characteristic (ROC) curve analysis and n-fold cross-validation. Results: The top radiomics features extracted from scans taken midway through treatment showed significant differences between the two responder groups (p-values < 0.0005). In contrast, those from pretreatment scans did not exhibit significant differences. The AUC of the mean ROC curve for the predictive model based on the top features from pretreatment scans was 0.8529, while it reached 0.9420 for those derived midway through treatment scans. Conclusions: The study highlights the potential of longitudinal FDG-PET radiomics extracted midway through treatment for predicting response to chemoradiation in LACC patients and emphasizes that interim PET scans could be crucial in personalized medicine, ultimately enhancing therapeutic outcomes for LACC.
Prospective Screening of Cancer Syndromes in Patients with Mesenchymal Tumors
Authors: Öfverholm Ingegerd , Lin Yingbo , Mondini Julia , Hardingz John , Bränström Robert , Tsagkozis Panagiotis , Wirta Valtteri , Gellerbring Anna , Lindberg Johan , Chellappa Venkatesh , Mayrhofer Markus , Haglund Cecilia , Haglund de Flon Felix , Wallander Karin
Published: November 2024

Background: The etiology of most mesenchymal tumors is unknown, and knowledge about syndromes with an increased risk of tumors in bone or soft tissue is sparse. Methods: We present a prospective germline analysis of 312 patients with tumors suspected of being sarcomas at a tertiary sarcoma center. Germline and tumor whole genome sequencing, tumor transcriptome, and methylome analyses were performed. Results: Germline pathogenic or likely pathogenic variants associated with an increased risk of tumors were detected in 24 patients (8%), of which 11 (4%) harbored a detectable second hit in the tumor. Second hits were confirmed in genes with (NF1, RB1, TP53, EXT2, and SDHC) and without (ATM, CDC73, MLH1, MSH6, POLG, and KCNQ1) known association with mesenchymal tumor predisposition. Sarcomas from two Lynch syndrome patients showed mismatch repair deficiency, predicting a treatment response to immune checkpoint inhibitors (Level 1 biomarker according to the FDA (Federal Drug Administration) and ESMO (European Society for Medical Oncology)). None of the three CHEK2 carriers had a second hit in the tumor, suggesting a weak link to sarcoma. Conclusions: We conclude that second-hit analyses can be used in standard of care to identify syndrome-related tumors. This approach can help distinguish true manifestations of tumor syndromes from unrelated germline findings and enhance the understanding of germline predisposition in soft tissue tumors. Prospective screening using germline whole genome sequencing should be considered when comprehensive somatic sequencing is introduced into clinical practice.
Identification of Molecular Subtypes and Prognostic Traits Based on Chromosomal Instability Phenotype-Related Genes in Lung Adenocarcinoma
Authors: Jeon Youngsic , Choi Kyung-Chul , Park Young Nyun , Kim Young-Joo
Published: November 2024

Lung adenocarcinoma (LUAD) exhibits significant molecular heterogeneity; however, previous studies have not fully explored its classification into distinct molecular subtypes. Here, we identified LUAD-significant chromosomal instability (CIN) phenotype genes (n = 24) using a TCGA-LUAD cohort (n = 592) and evaluated their ability to predict pathologic grade. Unsupervised clustering and principal component analysis revealed that LUAD patients could be classified into CIN phenotype-related subtypes (GroupLow, GroupModerate, and GroupHigh), each exhibiting distinct transcriptomic patterns. Notably, the GroupHigh showed significantly poor overall survival [OS; hazard ratio (HR) = 1.43, p-value < 10−3] and disease-free survival (DFS; HR = 1.27, p-value < 10−3). Univariate and multivariate analysis confirmed that its expression status was an independent prognostic predictor (p-value < 10−3, HR = 2.18, 95% C.I = 1.26–3.76) of the clinical outcomes, outperforming pathologic grade (p-value < 10−3, HR = 1.2, 95% C.I = 1.08–1.33). Moreover, analysis of surfactant metabolism-related genes revealed higher expression in the GroupLow, which was associated with a favorable prognosis. By integrating multiple independent cohorts (n = 779), we validated these findings and confirmed that CIN phenotype gene status serves as a critical prognostic marker in LUAD. Furthermore, genomic profiling showed that the GroupHigh exhibited frequent mutations in key genes such as KEAP1, LYST, SETD2, and TP53, with oncogenes in this group preferentially showing copy number gains. Our study highlights the significance of CIN phenotype gene status as a predictor of LUAD prognosis and its association with transcriptomic and genomic alterations, paving the way for further clinical validation and potential therapeutic interventions.
Accuracy of O-RADS System in Differentiating Between Benign and Malignant Adnexal Masses Assessed via External Validation by Inexperienced Gynecologists
Authors: Buranaworathitikul Peeradech , Wisanumahimachai Veera , Phoblap Natthaphon , Porngasemsart Yosagorn , Rugfoong Waranya , Yotchana Nuttha , Uthaichalanont Pakaporn , Jiampochaman Thunthida , Kunanukulwatana Chayanid , Thiamkaew Atiphoom , Luewan Suchaya , Tantipalakorn Charuwan , Tongsong Theera
Published: November 2024

Objective: To evaluate the accuracy of the O-RADS system in differentiating between benign and malignant adnexal masses, as assessed by inexperienced gynecologists. Methods: Ten gynecologic residents attended a 20 h training course on the O-RADS system conducted by experienced examiners. Following the training, the residents performed ultrasound examinations on patients admitted with adnexal masses under supervision, recording the data in a database that included videos and still images. The senior author later accessed this ultrasound database and presented the cases offline to ten residents for O-RADS rating, with the raters being blinded to the final diagnosis. The efficacy of the O-RADS system by the residents and inter-observer variability were assessed. Results: A total of 201 adnexal masses meeting the inclusion criteria were evaluated, consisting of 136 (67.7%) benign masses and 65 (32.3%) malignant masses. The diagnostic performance of the O-RADS system showed a sensitivity of 90.8% (95% CI: 82.2–96.2%) and a specificity of 86.8% (95% CI: 80.4–91.8%). Inter-observer variability in scoring was analyzed using multi-rater Fleiss Kappa analysis, yielding Kappa indices of 0.642 (95% CI: 0.641–0.643). The false positive rate was primarily due to the misclassification of solid components in classic benign masses as O-RADS-4 or O-RADS-5. Conclusions: The O-RADS system demonstrates high diagnostic performance in distinguishing benign from malignant adnexal masses, even when used by inexperienced examiners. However, the false positive rate remains relatively high, mainly due to the over-interpretation of solid-appearing components in classic benign lesions. Despite this, inter-observer variability among non-expert raters was substantial. Incorporating O-RADS system training into residency programs is beneficial for inexperienced practitioners. This study could be an educational model for gynecologic residency training for other systems of sonographic features.
Eco-Friendly TiO2 Nanoparticles: Harnessing Aloe Vera for Superior Photocatalytic Degradation of Methylene Blue
Authors: De Luca Agnese , De Benedetto Angelantonio , De Matteis Valeria , Cascione Mariafrancesca , Di Corato Riccardo , Ingrosso Chiara , Corrado Massimo , Rinaldi Rosaria
Published: November 2024

In recent years, the contamination of aquatic environments by organic chemicals, including dyes such as methylene blue (MB), Congo red, and crystal violet, has become an increasing concern, as has their treatment. In this work, titanium dioxide nanoparticles (TiO2 NPs) were studied for their photocatalytic performance by measuring the degradation of MB under UV light. TiO2 NPs were synthesized using two synthetic processes optimized in this study: a green method, namely leveraging the natural properties of Aloe vera leaf extract; and a conventional approach. The resulting NPs were thoroughly characterized using X-rays Diffraction (XRD), Transmission Electron Microscopy (TEM), Scanning Electron Microscopy (SEM), Brunauer–Emmett–Teller (BET), UV–Vis and ζ-potential analysis. The TiO2 NPs synthesized by the green method demonstrated a degradation efficiency of (50 ± 3)% after 180 min, which was significantly higher than the (16 ± 3)% achieved by NPs synthesized through the conventional route. Moreover, the reaction rate constant for the green-synthesized TiO2 NPs was found to be approximately five times greater than that of the conventionally synthesized NPs. These results open up a new scenario in the pollution removal strategy research, using resources accessible in nature to synthesize NPs with high photocatalytic activity, which could also be useful for other applications, such as hydrogen production.