bluuebunny/crossref_metadata_2025 huggingface dataset:
Case Report: Concomitant Massive Cerebral Venous Thrombosis and Internal Iliac Vein Thrombosis Related to Paucisymptomatic COVID-19 Infection
Authors: Beretta Simone , Da Re Fulvio , Francioni Valentina , Remida Paolo , Storti Benedetta , Fumagalli Lorenzo , Piatti Maria Luisa , Santoro Patrizia , Cereda Diletta , Cutellè Claudia , Pirro Fiammetta , Montisano Danilo Antonio , Beretta Francesca , Pasini Francesco , Cavallero Annalisa , Appollonio Ildebrando , Ferrarese Carlo
Published: February 2021

Thrombotic complications are common in COVID-19 patients, but cerebral venous system involvement, timing after infection, optimal treatment, and long-term outcome are uncertain. We report a case of massive cerebral venous thrombosis and concomitant internal iliac vein thrombosis occurring in the late phase of paucisymptomatic COVID-19 infection. Mild respiratory symptoms, without fever, started 3 weeks before headache and acute neurological deficits. The patient had silent hypoxemia and typical COVID-19 associated interstitial pneumonia. Brain CT scan showed a left parietal hypodense lesion with associated sulcal subarachnoid hemorrhage. CT cerebral venography showed a massive cerebral venous thrombosis involving the right transverse sinus, the right jugular bulb, the superior sagittal sinus, the straight sinus, the vein of Galen, and both internal cerebral veins. Abdominal CT scan showed no malignancy but revealed an asymptomatic right internal iliac vein thrombosis. Both cerebral venous thrombosis and pelvic vein thrombosis were effectively treated with unfractionated heparin started on the day of admission, then shifted to low molecular weight heparin, with a favorable clinical course. Nasopharyngel swab, repeated twice, tested negative for SARS-CoV-2. Serological tests confirmed SARS-CoV-2 infection. Our case supports active surveillance and prevention of thrombotic complications associated with COVID-19, which may affect both peripheral and cerebral venous system. Early initiation of unfractionated heparin may lead to good neurologic outcome.
Right Temporoparietal Junction Plays a Role in the Modulation of Emotional Mimicry by Group Membership
Authors: Peng Shenli , Kuang Beibei , Zhang Ling , Hu Ping
Published: February 2021

Our prior research demonstrated that the right temporoparietal junction (rTPJ) exerted a modulatory role in ingroup bias in emotional mimicry. In this study, two experiments were conducted to further explore whether the rTPJ is a neural region for emotional mimicry or for the modulation of emotional mimicry by group membership in a sham-controlled, double-blinded, between-subject design. Both experiments employed non-invasive transcranial direct current stimulation (tDCS) to temporarily change the cortical excitability over the rTPJ and facial electromyography (fEMG) to measure facial muscle activations as an index of emotional mimicry. After the anodal or sham stimulation, participants in Experiment 1 passively viewed a series of happy clips, while participants in Experiment 2 viewed happy clips performed by ethnic ingroup and outgroup models. fEMG analyses revealed that participants in Experiment 1 showed the same degree of happy mimicry for both tDCS conditions (anodal vs. sham) and participants in Experiment 2 showed an ingroup bias in happy mimicry in the sham condition, which disappeared in the anodal condition. Taken together, the present study demonstrated that rTPJ plays a role in the modulation of emotional mimicry by group membership.
Case Report: Untreatable Headache in a Child With Ventriculoperitoneal Shunt Managed by Use of New Non-invasive Intracranial Pressure Waveform
Authors: Paraguassu Geraldo , Khilnani Mark , Rabelo Nicollas Nunes , Cobos Luiza D'Ottaviano , Frigieri Gustavo
Published: February 2021

brain4care, a new Food and Drug Administration (FDA)-cleared non-invasive sensor that monitors intracranial pressure waveforms, was used in a 13-year-old girl who presented with untreatable headaches. The patient had a history of craniopharyngioma resection and a ventriculoperitoneal shunt placement 7 years prior to the use of the device. Secondary obstructive hydrocephalus was also a present factor in the case. The hypothesis was that due to the hydrocephalus, the child presented chronic headaches and needed constant readjustment into the ventriculoperitoneal shunt to regulate the cerebrospinal fluid inside her ventricles in order to control the patient's intracranial pressure (ICP). The device was chosen considering the risks to submit a patient into the regular invasive method to measure ICP. It was identified that the device could also indicate altered intracranial compliance due to the ratio between the P1 and P2 amplitudes (P2/P1 ratio > 1).
Dl-3-n-Butylphthalide Reduces Cognitive Deficits and Alleviates Neuropathology in P301S Tau Transgenic Mice
Authors: Chang Yanmin , Yao Yi , Ma Rong , Wang Zemin , Hu Junjie , Wu Yanqing , Jiang Xingjun , Li Lulu , Li Gang
Published: February 2021

Alzheimer’s disease (AD) is a destructive and burdensome neurodegenerative disease, one of the most common characteristics of which are neurofibrillary tangles (NFTs) that are composed of abnormal tau protein. Animal studies have suggested that dl-3-n-butylphthalide (dl-NBP) alleviates cognitive impairment in mouse models of APP/PS1 and SAMP8. However, the underlying mechanisms related to this remain unclear. In this study, we examined the effects of dl-NBP on learning and memory in P301S transgenic mice, which carry the human tau gene with the P301S mutation. We found that dl-NBP supplementation effectively improved behavioral deficits and rescued synaptic loss in P301S tau transgenic mice, compared with vehicle-treated P301S mice. Furthermore, we also found that it markedly inhibited the hyperphosphorylated tau at the Ser262 site and decreased the activity of MARK4, which was associated with tau at the Ser262 site. Finally, dl-NBP treatment exerted anti-inflammatory effects and reduced inflammatory responses in P301S mice. In conclusion, our results provide evidence that dl-NBP has a promising potential for the therapy of tauopathies, including AD.
Western Diet Consumption During Development: Setting the Stage for Neurocognitive Dysfunction
Authors: Tsan Linda , Décarie-Spain Léa , Noble Emily E. , Kanoski Scott E.
Published: February 2021

The dietary pattern in industrialized countries has changed substantially over the past century due to technological advances in agriculture, food processing, storage, marketing, and distribution practices. The availability of highly palatable, calorically dense foods that are shelf-stable has facilitated a food environment where overconsumption of foods that have a high percentage of calories derived from fat (particularly saturated fat) and sugar is extremely common in modern Westernized societies. In addition to being a predictor of obesity and metabolic dysfunction, consumption of a Western diet (WD) is related to poorer cognitive performance across the lifespan. In particular, WD consumption during critical early life stages of development has negative consequences on various cognitive abilities later in adulthood. This review highlights rodent model research identifying dietary, metabolic, and neurobiological mechanisms linking consumption of a WD during early life periods of development (gestation, lactation, juvenile and adolescence) with behavioral impairments in multiple cognitive domains, including anxiety-like behavior, learning and memory function, reward-motivated behavior, and social behavior. The literature supports a model in which early life WD consumption leads to long-lasting neurocognitive impairments that are largely dissociable from WD effects on obesity and metabolic dysfunction.
Comparison of Survival Between Primary Debulking Surgery Versus Neoadjuvant Chemotherapy for Ovarian Cancers in a Personalized Treatment Cohort
Authors: Feng Zheng , Wen Hao , Li Ruimin , Liu Shuai , Fu Yi , Chen Xiaojun , Bi Rui , Ju Xingzhu , Wu Xiaohua
Published: February 2021

Objective

To compare survival between primary debulking surgery (PDS) and neoadjuvant chemotherapy (NACT) for the treatment of ovarian cancer patients per our selective protocol.

Methods

Between Sep 1st, 2015, and Aug 31st, 2017, 161 patients were enrolled in our prospective cohort. All of the patients received preoperative clinic-radiological assessments, according to the Suidan criteria for R0 resection. Patients with a score of 0–2 received PDS. Patients with a score of ≥3 were counseled on the choices of PDS, NACT, or an optional staging laparoscopy, according to the Fagotti criteria. Clinic-pathological data were prospectively collected until May 1st, 2020, and the impacts of different treatment strategies on progression-free survival (PFS) and overall survival (OS) were analyzed.

Results

110 patients underwent PDS, and 51 patients received NACT with consequent interval debulking surgery. The R0 resection rate was 57.8%. All but one of the patients received platinum-based chemotherapy, and 105 (65.2%) patients were platinum-sensitive. Based on the univariate analysis, the PDS group exhibited prolonged PFS compared with the NACT group (P=0.029). The subgroup analysis showed that patients receiving NACT with residual disease (RD) exhibited the worst PFS (P=0.001). Based on the multivariate analysis, NACT with RD was still an independent impaired factor for PFS (P=0.04). However, NACT did not affect OS in the univariate or multivariate analyses.

Conclusion

In our prospective cohort, NACT ovarian patients exhibited inferior PFS and noninferior OS compared with PDS patients. Given our selective protocol, NACT cannot be arbitrarily denied while appropriate PDS is still a priority.
Intubation Related Laryngeal Injuries in Pediatric Population
Authors: Lambercy Karma , Pincet Laurence , Sandu Kishore
Published: February 2021

Introduction: Laryngeal intubation related lesions (LIRL) in pediatric patients cause extreme morbidity in both elective and emergency settings. It has a wide range of presentations from minor laryngeal edema to a life-threatening airway obstruction. We report here our units' experience with LIRL in neonates, infants, and small children.

Material and Methods: This is a retrospective monocentric cohort study between January 2013 and April 2019.

Results: Thirty-nine patients with intubation lesions were included in the study. We looked at the lesions type, characteristics, management, and outcome. Half the patients were premature and having comorbidities. Main LIRL were subglottic stenosis (31%), ulcers (26%), granulations (18%), retention cysts (18%), posterior glottic stenosis (13%), and vocal cords edema (5%). Unfavorable lesions causing airway stenosis were associated with an intubation duration of over 1 week and were an important factor in causing airway stenosis (p < 0.05). The endoscopic treatment performed for these lesions was lesion and anatomical site-specific. Tracheostomy was needed in five patients, and was avoided in another two. Seven patients (18%) received open surgery prior to their decannulation.

Conclusions: LIRL management is challenging and stressful in the pediatric population and optimal treatment could avoid extreme morbidity in them. Intubation duration and associated comorbidities are important factors in deciding the severity of these lesions. Protocols to prevent the formation of these lesions are critical.
Two Targets, One Hit: new Anticancer Therapeutics to Prevent Tumorigenesis Without Cardiotoxicity
Authors: Szabó Zoltán , Hornyák Lilla , Miskei Márton , Székvölgyi Lóránt
Published: February 2021

A serious adverse effect of cancer therapies is cardiovascular toxicity, which significantly limits the widespread use of antineoplastic agents. The promising new field of cardio-oncology offers the identification of potent anti-cancer therapeutics that effectively inhibit cancer cell proliferation without causing cardiotoxicity. Future introduction of recently identified cardio-safe compounds into clinical practice (including ERK dimerization inhibitors or BAX allosteric inhibitors) is expected to help oncologists avoid unwanted cardiological complications associated with therapeutic interventions.
Early Exposure to Water Turbidity Affects Visual Capacities in Cuttlefish (Sepia officinalis)
Authors: Goerger Alice , Darmaillacq Anne-Sophie , Shashar Nadav , Dickel Ludovic
Published: February 2021

In La Manche (English Channel) the level of turbidity changes, not only seasonally and daily in seawater but also along the coast. As a consequence, vision in marine species is limited when based only on contrast-intensity. It is hypothesized that polarization sensitivity (PS) may help individuals detect preys and predators in turbid environments. In the cuttlefish,Sepia officinalis, to date, all behavioral studies have been conducted on animals reared in clear water. But the cuttlefish sensory system is adapted to a range of turbid environments. Our hypothesis was that rearing cuttlefish in clear water may affect the development of their visual system, and potentially affect their visually guided behaviors. To test this, newly-hatched cuttlefish, from eggs laid by females brought in from the wild, were reared for 1 month under three different conditions: clear water (C group), low turbidity (0.1 g / l of clay, 50–80 NTU, LT group) and high turbidity (0.5 g / l of clay, 300–400 NTU, HT group). The visual capacities of cuttlefish were tested with an optomotor apparatus at 7 days and at 1 month post-hatching. Optomotor responses of juveniles were measured by using three screen patterns (black and white stripes, linearly polarized stripes set at different orientations, and a uniform gray screen). Optomotor responses of juveniles suggest that exposure to turbid water improves the development of their PS when tested in clear water (especially in LT group) but not when tested in turbid water. We suggest that the use of slightly turbid water in rearing systems may improve the development of vision in young cuttlefish with no detrimental effect to their survival rate. Future research will consider water turbidity as a possible factor for the improvement of cuttlefish well-being in artificial rearing systems.
Genome-Wide Association Study of Topsoil Root System Architecture in Field-Grown Soybean [Glycine max (L.) Merr.]
Authors: Dhanapal Arun Prabhu , York Larry M. , Hames Kasey A. , Fritschi Felix B.
Published: February 2021

Water and nutrient acquisition is a critical function of plant root systems. Root system architecture (RSA) traits are often complex and controlled by many genes. This is the first genome-wide association study reporting genetic loci for RSA traits for field-grown soybean (Glycine max). A collection of 289 soybean genotypes was grown in three environments, root crowns were excavated, and 12 RSA traits assessed. The first two components of a principal component analysis of these 12 traits were used as additional aggregate traits for a total of 14 traits. Marker–trait association for RSA traits were identified using 31,807 single-nucleotide polymorphisms (SNPs) by a genome-wide association analysis. In total, 283 (non-unique) SNPs were significantly associated with one or more of the 14 root traits. Of these, 246 were unique SNPs and 215 SNPs were associated with a single root trait, while 26, four, and one SNPs were associated with two, three, and four root traits, respectively. The 246 SNPs marked 67 loci associated with at least one of the 14 root traits. Seventeen loci on 13 chromosomes were identified by SNPs associated with more than one root trait. Several genes with annotation related to processes that could affect root architecture were identified near these 67 loci. Additional follow-up studies will be needed to confirm the markers and candidate genes identified for RSA traits and to examine the importance of the different root characteristics for soybean productivity under a range of soil and environmental conditions.
Dual Roles of GSNOR1 in Cell Death and Immunity in Tetraploid Nicotiana tabacum
Authors: Li Zhen-Chao , Ren Qian-Wei , Guo Yan , Ran Jie , Ren Xiao-Tian , Wu Ni-Ni , Xu Hui-Yang , Liu Xia , Liu Jian-Zhong
Published: February 2021

S-nitrosoglutathione reductase 1 (GSNOR1) is the key enzyme that regulates cellular homeostasis of S-nitrosylation. Although extensively studied in Arabidopsis, the roles of GSNOR1 in tetraploid Nicotiana species have not been investigated previously. To study the function of NtGSNOR1, we knocked out two NtGSNOR1 genes simultaneously in Nicotiana tabacum using clustered regularly interspaced short palindromic repeats (CRISPR)/caspase 9 (Cas9) technology. To our surprise, spontaneous cell death occurred on the leaves of the CRISPR/Cas9 lines but not on those of the wild-type (WT) plants, suggesting that NtGSNOR1 negatively regulates cell death. The natural cell death on the CRISPR/Cas9 lines could be a result from interactions between overaccumulated nitric oxide (NO) and hydrogen peroxide (H2O2). This spontaneous cell death phenotype was not affected by knocking out two Enhanced disease susceptibility 1 genes (NtEDS11a/1b) and thus was independent of the salicylic acid (SA) pathway. Unexpectedly, we found that the NtGSNOR1a/1b knockout plants displayed a significantly (p < 0.001) enhanced resistance to paraquat-induced cell death compared to WT plants, suggesting that NtGSNOR1 functions as a positive regulator of the paraquat-induced cell death. The increased resistance to the paraquat-induced cell death of the NtGSNOR1a/1b knockout plants was correlated with the reduced level of H2O2 accumulation. Interestingly, whereas the N gene-mediated resistance to Tobacco mosaic virus (TMV) was significantly enhanced (p < 0.001), the resistance to Pseudomonas syringae pv. tomato DC3000 was significantly reduced (p < 0.01) in the NtGSNOR1a/1b knockout lines. In summary, our results indicate that NtGSNOR1 functions as both positive and negative regulator of cell death under different conditions and displays distinct effects on resistance against viral and bacterial pathogens.
Unraveling the Strategies Used by the Underexploited Amaranth Species to Confront Salt Stress: Similarities and Differences With Quinoa Species
Authors: Estrada Yanira , Fernández-Ojeda Amanda , Morales Belén , Egea-Fernández José M. , Flores Francisco B. , Bolarín María C. , Egea Isabel
Published: February 2021

Yield losses due to cultivation in saline soils is a common problem all over the world as most crop plants are glycophytes and, hence, susceptible to salt stress. The use of halophytic crops could be an interesting alternative to cope with this issue. The Amaranthaceae family comprises by far the highest proportion of salt-tolerant halophytic species. Amaranth and quinoa belong to this family, and their seeds used as pseudo-cereal grains have received much attention in recent years because of their exceptional nutritional value. While advances in the knowledge of salt tolerance mechanisms of quinoa have been remarkable in recent years, much less attention was received by amaranth, despite evidences pointing to amaranth as a promising species to be grown under salinity. In order to advance in the understanding of strategies used by amaranth to confront salt stress, we studied the comparative responses of amaranth and quinoa to salinity (100 mM NaCl) at the physiological, anatomical, and molecular levels. Amaranth was able to exhibit salt tolerance throughout its life cycle, since grain production was not affected by the saline conditions applied. The high salt tolerance of amaranth is associated with a low basal stomatal conductance due to a low number of stomata (stomatal density) and degree of stomata aperture (in adaxial surface) of leaves, which contributes to avoid leaf water loss under salt stress in a more efficient way than in quinoa. With respect to Na+ homeostasis, amaranth showed a pattern of Na+ distribution throughout the plant similar to glycophytes, with the highest accumulation found in the roots, followed by the stem and the lowest one detected in the leaves. Contrarily, quinoa exhibited a Na+ includer character with the highest accumulation detected in the shoots. Expression levels of main genes involved in Na+ homeostasis (SOS1, HKT1s, and NHX1) showed different patterns between amaranth and quinoa, with a marked higher basal expression in amaranth roots. These results highlight the important differences in the physiological and molecular responses of amaranth and quinoa when confronted with salinity.
Update on the Roles of Polyamines in Fleshy Fruit Ripening, Senescence, and Quality
Authors: Gao Fan , Mei Xurong , Li Yuzhong , Guo Jiaxuan , Shen Yuanyue
Published: February 2021

Ripening of fleshy fruits involves complex physiological, biochemical, and molecular processes that coincide with various changes of the fruit, including texture, color, flavor, and aroma. The processes of ripening are controlled by ethylene in climacteric fruits and abscisic acid (ABA) in non-climacteric fruits. Increasing evidence is also uncovering an essential role for polyamines (PAs) in fruit ripening, especially in climacteric fruits. However, until recently breakthroughs have been made in understanding PA roles in the ripening of non-climacteric fruits. In this review, we compare the mechanisms underlying PA biosynthesis, metabolism, and action during ripening in climacteric and non-climacteric fruits at the physiological and molecular levels. The PA putrescine (Put) has a role opposite to that of spermidine/spermine (Spd/Spm) in cellular metabolism. Arginine decarboxylase (ADC) is crucial to Put biosynthesis in both climacteric and non-climacteric fruits. S-adenosylmethionine decarboxylase (SAMDC) catalyzes the conversion of Put to Spd/Spm, which marks a metabolic transition that is concomitant with the onset of fruit ripening, induced by Spd in climacteric fruits and by Spm in non-climacteric fruits. Once PA catabolism is activated by polyamine oxidase (PAO), fruit ripening and senescence are facilitated by the coordination of mechanisms that involve PAs, hydrogen peroxide (H2O2), ABA, ethylene, nitric oxide (NO), and calcium ions (Ca2+). Notably, a signal derived from PAO5-mediated PA metabolism has recently been identified in strawberry, a model system for non-climacteric fruits, providing a deeper understanding of the regulatory roles played by PAs in fleshy fruit ripening.
Characterization of Two Zygnema Strains (Zygnema circumcarinatum SAG 698-1a and SAG 698-1b) and a Rapid Method to Estimate Nuclear Genome Size of Zygnematophycean Green Algae
Authors: Feng Xuehuan , Holzinger Andreas , Permann Charlotte , Anderson Dirk , Yin Yanbin
Published: February 2021

Zygnematophyceae green algae (ZGA) have been shown to be the closest relatives of land plants. Three nuclear genomes (Spirogloea muscicola, Mesotaenium endlicherianum, and Penium margaritaceum) of ZGA have been recently published, and more genomes are underway. Here we analyzed two Zygnema circumcarinatum strains SAG 698-1a (mating +) and SAG 698-1b (mating −) and found distinct cell sizes and other morphological differences. The molecular identities of the two strains were further investigated by sequencing their 18S rRNA, psaA and rbcL genes. These marker genes of SAG 698-1a were surprisingly much more similar to Z. cylindricum (SAG 698-2) than to SAG 698-1b. Phylogenies of these marker genes also showed that SAG 698-1a and SAG 698-1b were well separated into two different Zygnema clades, where SAG 698-1a was clustered with Z. cylindricum, while SAG 698-1b was clustered with Z. tunetanum. Additionally, physiological parameters like ETRmax values differed between SAG 698-1a and SAG 698-1b after 2 months of cultivation. The de-epoxidation state (DEPS) of the xanthophyll cycle pigments also showed significant differences. Surprisingly, the two strains could not conjugate, and significantly differed in the thickness of the mucilage layer. Additionally, ZGA cell walls are highly enriched with sticky and acidic polysaccharides, and therefore the widely used plant nuclear extraction protocols do not work well in ZGA. Here, we also report a fast and simple method, by mechanical chopping, for efficient nuclear extraction in the two SAG strains. More importantly, the extracted nuclei were further used for nuclear genome size estimation of the two SAG strains by flow cytometry (FC). To confirm the FC result, we have also used other experimental methods for nuclear genome size estimation of the two strains. Interestingly, the two strains were found to have very distinct nuclear genome sizes (313.2 ± 2.0 Mb in SAG 698-1a vs. 63.5 ± 0.5 Mb in SAG 698-1b). Our multiple lines of evidence strongly indicate that SAG 698-1a possibly had been confused with SAG 698-2 prior to 2005, and most likely represents Z. cylindricum or a closely related species.
Chromosomal Mapping of Tandem Repeats Revealed Massive Chromosomal Rearrangements and Insights Into Senna tora Dysploidy
Authors: Waminal Nomar Espinosa , Pellerin Remnyl Joyce , Kang Sang-Ho , Kim Hyun Hee
Published: February 2021

Tandem repeats can occupy a large portion of plant genomes and can either cause or result from chromosomal rearrangements, which are important drivers of dysploidy-mediated karyotype evolution and speciation. To understand the contribution of tandem repeats in shaping the extant Senna tora dysploid karyotype, we analyzed the composition and abundance of tandem repeats in the S. tora genome and compared the chromosomal distribution of these repeats between S. tora and a closely related euploid, Senna occidentalis. Using a read clustering algorithm, we identified the major S. tora tandem repeats and visualized their chromosomal distribution by fluorescence in situ hybridization. We identified eight independent repeats covering ~85 Mb or ~12% of the S. tora genome. The unit lengths and copy numbers had ranges of 7–5,833 bp and 325–2.89 × 106, respectively. Three short duplicated sequences were found in the 45S rDNA intergenic spacer, one of which was also detected at an extra-NOR locus. The canonical plant telomeric repeat (TTTAGGG)n was also detected as very intense signals in numerous pericentromeric and interstitial loci. StoTR05_180, which showed subtelomeric distribution in Senna occidentalis, was predominantly pericentromeric in S. tora. The unusual chromosomal distribution of tandem repeats in S. tora not only enabled easy identification of individual chromosomes but also revealed the massive chromosomal rearrangements that have likely played important roles in shaping its dysploid karyotype.
Predicting Tree Species From 3D Laser Scanning Point Clouds Using Deep Learning
Authors: Seidel Dominik , Annighöfer Peter , Thielman Anton , Seifert Quentin Edward , Thauer Jan-Henrik , Glatthorn Jonas , Ehbrecht Martin , Kneib Thomas , Ammer Christian
Published: February 2021

Automated species classification from 3D point clouds is still a challenge. It is, however, an important task for laser scanning-based forest inventory, ecosystem models, and to support forest management. Here, we tested the performance of an image classification approach based on convolutional neural networks (CNNs) with the aim to classify 3D point clouds of seven tree species based on 2D representation in a computationally efficient way. We were particularly interested in how the approach would perform with artificially increased training data size based on image augmentation techniques. Our approach yielded a high classification accuracy (86%) and the confusion matrix revealed that despite rather small sample sizes of the training data for some tree species, classification accuracy was high. We could partly relate this to the successful application of the image augmentation technique, improving our result by 6% in total and 13, 14, and 24% for ash, oak and pine, respectively. The introduced approach is hence not only applicable to small-sized datasets, it is also computationally effective since it relies on 2D instead of 3D data to be processed in the CNN. Our approach was faster and more accurate when compared to the point cloud-based “PointNet” approach.
ABAP1 Plays a Role in the Differentiation of Male and Female Gametes in Arabidopsis thaliana
Authors: Cabral Luiz M. , Masuda Hana P. , Ballesteros Helkin F. , de Almeida-Engler Janice , Alves-Ferreira Márcio , De Toni Karen L. G. , Bizotto Fernanda M. , Ferreira Paulo C. G. , Hemerly Adriana S.
Published: February 2021

The correct development of a diploid sporophyte body and a haploid gametophyte relies on a strict coordination between cell divisions in space and time. During plant reproduction, these divisions have to be temporally and spatially coordinated with cell differentiation processes, to ensure a successful fertilization. Armadillo BTB Arabidopsis protein 1 (ABAP1) is a plant exclusive protein that has been previously reported to control proliferative cell divisions during leaf growth in Arabidopsis. Here, we show that ABAP1 binds to different transcription factors that regulate male and female gametophyte differentiation, repressing their target genes expression. During male gametogenesis, the ABAP1-TCP16 complex repressesCDT1btranscription, and consequently regulates microspore first asymmetric mitosis. In the female gametogenesis, the ABAP1-ADAP complex repressesEDA24-liketranscription, regulating polar nuclei fusion to form the central cell. Therefore, besides its function during vegetative development, this work shows that ABAP1 is also involved in differentiation processes during plant reproduction, by having a dual role in regulating both the first asymmetric cell division of male gametophyte and the cell differentiation (or cell fusion) of female gametophyte.
Safeguarding the Therapeutic Alliance: Managing Disaffiliation in the Course of Work With Psychotherapeutic Projects
Authors: Guxholli Aurora , Voutilainen Liisa , Peräkylä Anssi
Published: February 2021

Therapeutic alliance is a central concept in psychotherapeutic work. The relationship between the therapist and the patient plays an important role in the therapeutic process and outcome. In this article, we investigate how therapists work with disaffiliation resulting from enduring disagreement while maintaining an orientation to the psychotherapeutic project at hand. Data come from a total of 18 sessions of two dyads undergoing psychoanalytic psychotherapy and is analyzed with conversation analysis. We found that collaborative moves deployed amidst enduring disagreement can assist the therapist in furthering the disagreement as part of the ongoing psychotherapeutic project. Relying on their collaborative format, therapists utilize collaborative moves to temporarily mend the disaffiliation without necessarily changing their position and re-affiliating with the patient. We show how the relation between the therapist and the patient gets transformed in the moment-by-moment work accomplished in the psychotherapeutic talk.
Contextual and Psychological Predictors of Militant Extremist Mindset in Youth
Authors: Vukčević Marković Maša , Nicović Aleksandra , Živanović Marko
Published: February 2021

The present study aims to identify contextual and psychological factors of proneness to radicalization and violent extremism (RVE) operationalized through the Militant Extremist Mindset scale (MEM) consisting of three distinct aspects: Proviolence (PV), Vile World beliefs (VW), and trust in Divine Power (DP). A community sample of 271 high school students (72% females) from Belgrade and Sandžak regions in Serbia completed: (1) a 24-item MEM scale; (2) contextual measures including a 6-item scale of family dysfunction (FDys) and a 4-item composite measure capturing exposure to a harsh school environment and peer abuse (HSE); (3) psychological measures including the 9-item Right-Wing Authoritarianism scale (RWA), the 5-item Social Dominance Orientation scale (SDO), and the 20-item UCLA Loneliness scale (LON). A path analysis was conducted with contextual factors on the first and psychological factors on the second level of the model predicting the three factors of MEM. LON was positively predicted by FDys and HSE, SDO by HSE only, while RWA was positively predicted by FDys only. Contextual and psychological factors accounted for 27% of the variance in PV (LON, SDO), 15% of the variance in VW (FDys, SDO), and 31% of the variance in DP (RWA). Obtained findings reveal a complex interplay of contextual and psychological drivers in the prediction of different aspects of RVE and build upon existing knowledge on risk factors associated with RVE.
Validation of the 10-Item Connor–Davidson Resilience Scale: The Case of Russian Youth
Authors: Nartova-Bochaver Sofya , Korneev Aleksei , Bochaver Konstantin
Published: February 2021

This study validates the 10-item Connor–Davidson Resilience Scale (CD-RISC-10) on a Russian youth sample. A total of 689 respondents participated (Mage = 20.22, SDage = 2.08; 526 females). The Warwick–Edinburgh Mental Well-being Scale, the International Positive and Negative Affect Schedule Short-Form, the Centre of Epidemiological Studies-Depression Scale, the Rosenberg Self-Esteem Scale, and the Authenticity Scale were used to examine the content validity of CD-RISC-10. Two hypotheses were examined: that the Russian version of the CD-RISC-10 (1) has structural validity (is unifactorial, as the original version) and (2) has convergent validity (which is proven by positive connections with psychological well-being and negative connections with ill-being). According to confirmatory factor analysis (CFA), it was shown that the scale really had a unifactorial structure; its reliability was satisfactory (α =.85, ωh =.84). No age trends in the CD-RISC-10 scores were detected; in males, the scores were higher than in females. As expected, CD-RISC-10 was positively connected with mental well-being, positive affect, self-esteem, and authentic living while negatively connected with depressive symptoms, negative affect, acceptance of external influence, and self-alienation. The Russian version of CD-RISC-10 seems to be a valid, stable, and reliable instrument which may be recommended for use in various areas of research and practice.
An Observational Cohort of First Episode Psychosis in Iran: The Azeri Recent Onset Acute Phase Psychosis Survey (ARAS Cohort) Study Protocol
Authors: Farhang Sara , Ghaemmaghami Mehrdad , Shafiee-Kandjani Ali Reza , Noorazar Seyed Gholamreza , Veling Wim , Malek Ayyoub , Somi Mohammad Hossein , Bruggeman Richard , Alizadeh Behrooz Z.
Published: February 2021

Background: Most of our knowledge about the etiology, course, treatment, and outcome of schizophrenia spectrum and other psychotic disorders stems from Western countries. Data from populations living in other geographical areas and low- and middle-income countries, with different genomes (ethnicity) and exposomes (e.g., culture and social support, drugs of abuse, religion), will add to our knowledge of this complex disorder.

Methods: The Azeri Acute phase/Recent onset psychosis Survey (ARAS) has been initiated to study the course of the disorder in patients with recent-onset psychosis using validated diagnostic tools and a comprehensive outcome monitoring system, aiming to reveal indicators for understanding the risk and resilience factors and for choosing the best-personalized treatment strategy. All participants will be evaluated for clinical signs and symptoms as well as risk and resilience factors and will be followed up for 1, 3, and 5 years for outcomes in several domains. A hierarchical cluster method will be applied to identify the number of clusters for each outcome. Defined models will be applied to assess the predictive value of cognition on symptomatic and functional outcomes at follow-up.

Discussion: The ARAS cohort will yield significant academic- (research and education) and care-related achievements. ARAS data and experience will have value both in being a useful model for other parts of this region and in an expansion of the currently available knowledge.
The “Gender Agenda” in Agriculture for Development and Its (Lack of) Alignment With Feminist Scholarship
Authors: Farhall Kate , Rickards Lauren
Published: February 2021

Sustainable food systems require sustainable agriculture. To achieve this, we argue, inclusive approaches are required that incorporate the voices and lived experiences of diverse social groups. In agriculture-based international development efforts (known as Agriculture for Development or A4D), it is increasingly being recognized that sustainable agriculture requires attention to gendered power relations. In the past, gender inequality has been a major barrier to developing inclusive, sustainable food systems, and continues to be so today. At the same time, however, gender is increasingly “on the agenda” in A4D. Yet what sort of agenda is being promoted and to what extent does it reflect progress in feminist scholarship? We examine the burgeoning “gender agenda” through the lens of policy materials produced by prominent A4D organizations. In doing so, we find problematic narratives that instrumentalise women in the name of sustainable agricultural development. However, we also find other more transformative discourses that, in troubling the drivers of gender inequality and promoting shared responsibility for change, reflect a deeper awareness of feminist scholarship. In any effort to advance sustainable agriculture, further progress is needed to address the myriad ways gender pervades not just development settings but development institutions and donor nations, and contributes to the production of as well as responses to global A4D challenges.
Constant-Time Complete Visibility for Robots with Lights: The Asynchronous Case
Authors: Sharma Gokarna , Vaidyanathan Ramachandran , Trahan Jerry L.
Published: February 2021

We consider the distributed setting of N autonomous mobile robots that operate in Look-Compute-Move (LCM) cycles and use colored lights (the robots with lights model). We assume obstructed visibility where a robot cannot see another robot if a third robot is positioned between them on the straight line segment connecting them. In this paper, we consider the problem of positioning N autonomous robots on a plane so that every robot is visible to all others (this is called the Complete Visibility problem). This problem is fundamental, as it provides a basis to solve many other problems under obstructed visibility. In this paper, we provide the first, asymptotically optimal, O(1) time, O(1) color algorithm for Complete Visibility in the asynchronous setting. This significantly improves on an O(N)-time translation of the existing O(1) time, O(1) color semi-synchronous algorithm to the asynchronous setting. The proposed algorithm is collision-free, i.e., robots do not share positions, and their paths do not cross. We also introduce a new technique for moving robots in an asynchronous setting that may be of independent interest, called Beacon-Directed Curve Positioning.
Technical Infrastructure as an Element of Sustainable Development of Rural Regions in Małopolskie Voivodeship in Poland and Trnava Region in Slovakia
Authors: Surówka Marcin , Popławski Łukasz , Fidlerová Helena
Published: February 2021

The work discusses issues of the infrastructure, its instruments, and specifics of infrastructure in Polish and Slovak rural areas. The aim of this article is to analyze the level of technical infrastructure development in rural regions of the Małopolskie Voivodeship in Poland and the west part of Slovakia—Trnava self-governing region (Trnava region) as two regions with a similar position regarding regional competitiveness index. Following the topic, after identification of strengths and weaknesses of mentioned regions, the opportunities, and threats of sustainable development of infrastructure in rural areas have been analyzed using the SWOT method. The development of sustainable, reliable, and functional infrastructure does not only refer to the chosen regions of Poland and Slovakia but also other regions in the European Union. Sustainable infrastructure is a factor stimulating social and economic progress as one of the most important determinants of sustainable development and regional competitiveness. The authors notice a particular lack in the sustainable development of infrastructure in the field of water and sewerage management together with the supply of water. Therefore, this article tries to complete the gap in research focusing on the concept of a more systematic approach to technical infrastructure improvement in the context of sustainable development, and strategy of cooperation.
Influence of Soil Properties and Initial Concentration on the Fractionation of Nickel, Zinc, Copper and Lead in Soils Derived from Different Parent Materials
Authors: Campillo-Cora Claudia , Rodríguez-González Laura , Arias-Estévez Manuel , Fernández-Calviño David , Soto-Gómez Diego
Published: February 2021

Different fractions of Ni, Zn, Cu and Pb were determined in metal-spiked forest soils derived from four parent materials using three extractants (H2O, CaCl2 and diethylenetriaminepentaacetic acid (DTPA)). It is important to determine how parent materials and soil properties affect the retention of these metals in order to predict their behavior and act accordingly in the event of accidental spillage, for example. The extraction of fractions was not sequential (before carrying out the extractions, the soil samples were divided into three parts), so the CaCl2 fraction also included the H2O one, and the DTPA fraction contained the other two. With the results, we developed models to predict the extraction of each fraction employing the physicochemical characteristics of the soil (e.g., pH, organic matter content and texture values) and the amount of metal added. The objective of this work was to determine how the properties of the soil would influence the fractioning of the metals considered, and through these characteristics create models to predict the behavior of each metal fraction. We found correlations between the different fractions of Ni and Zn, suggesting that there are soil properties that condition the retention of both metals. Pb and Cu showed different behavior than Zn or Ni, since the proportions extracted by H2O and CaCl2 were much lower. Regarding the DTPA fraction, unlike the case of Ni or Zn, the extraction of Cu and Pb was more homogeneous; they did not show great variation in different soils, even when considering the results of extraction in limestone soils. This may be due to the fact that the soil properties do not exert an important effect on their availability, or these two metals are considerably sensitive to the effect of pH, and no differences were observed because the extraction of the DTPA fraction was conducted with a buffered solution. For each fraction of metal used, we obtained a model with R2 always greater than 0.65. Considering these results, we can conclude that it is possible to predict Zn, Ni, Cu and Pb availability in soils developed on different parent materials. This can be achieved by identifying some basic soil characteristics and applying the developed equations.
Light Quality Affects Water Use of Sweet Basil by Changing Its Stomatal Development
Authors: Lim Sungeun , Kim Jongyun
Published: February 2021

Different light qualities affect plant growth and physiological responses, including stomatal openings. However, most researchers have focused on stomatal responses to red and blue light only, and the direct measurement of evapotranspiration has not been examined. Therefore, we quantified the evapotranspiration of sweet basil under various red (R), green (G), and blue (B) combinations using light-emitting diodes (LEDs) and investigated its stomatal responses. Seedlings were subjected to five different spectral treatments for two weeks at a photosynthetic photon flux density of 200 µmol m−2 s−1. The ratios of the RGB light intensities were as follows: R 100% (R100), R:G = 75:25 (R75G25), R:B = 75:25 (R75B25), R:G:B = 60:20:20 (R60G20B20), and R:G:B = 31:42:27 (R31G42B27). During the experiment, the evapotranspiration of the plants was measured using load cells. Although there were no significant differences in growth parameters among the treatments, the photosynthetic rate and stomatal conductance were higher in plants grown under blue LEDs (R75B25, R60G20B20, and R31G42B27) than in the R100 treatment. The amount of water used was different among the treatments (663.5, 726.5, 728.7, 778.0, and 782.1 mL for the R100, R75G25, R60G20B20, R75B25, and R31G42B27 treatments, respectively). The stomatal density was correlated with the blue light intensity (p = 0.0024) and with the combined intensity of green and blue light (p = 0.0029); therefore, green light was considered to promote the stomatal development of plants together with blue light. Overall, different light qualities affected the water use of plants by regulating stomatal conductance, including changes in stomatal density.
Amelioration Effect of LED Lighting in the Bioactive Compounds Synthesis during Carrot Sprouting
Authors: Martínez-Zamora Lorena , Castillejo Noelia , Gómez Perla A. , Artés-Hernández Francisco
Published: February 2021

Background: This work investigates the morphological and compositional changes of carrots sprouts during 17 days at 20 °C. Methods: Growing conditions were 7 days in darkness (dD) followed by 3, 7, or 10 days of a 16 h light/8 h darkness photoperiod (dP). Light stimuli used were fluorescent light (Fl), Blue+Red (B+R), Blue+Red+Far-Red (B+R+FR) Light-Emitting Diodes—LEDs- and darkness as control. Results: Results showed that lighting conditions improved the total antioxidant activity and increased the bioactive compounds compared to darkness treatment. However, hypocotyl and sprout length were increased under darkness conditions. Both LEDs treatments (B+R and B+R+FR) increased the phenolic content (phenolic acids and rutin) by 45% and 65% compared to darkness and by 32% regarding Fl. Moreover, a similar trend was observed in the carotenoids content under B+R LEDs, but not when FR was added. Conclusions: Our results suggest that LED lighting during carrot sprouting improved the synthesis of health-promoting compounds.
Comparative Anatomy of the Nasal Cavity in the Common Dolphin Delphinus delphis L., Striped Dolphin Stenella coeruleoalba M. and Pilot Whale Globicephala melas T.: A Developmental Study
Authors: García de los Ríos y Loshuertos Alvaro , Soler Laguía Marta , Arencibia Espinosa Alberto , López Fernández Alfredo , Covelo Figueiredo Pablo , Martínez Gomariz Francisco , Sánchez Collado Cayetano , García Carrillo Nuria , Ramírez Zarzosa Gregorio
Published: February 2021

Our goal was to analyze the main anatomical structures of the dolphin external nose and nasal cavity from fetal developmental stages to adult. Endoscopy was used to study the common development of the external nose and the melon, and nasal mucosa. Magnetic resonance imaging (MRI) and anatomical sections were correlated with anatomical sections. Computed tomography (CT) was used to generate 3D reconstructions of the nasal bones and nasal cavities to study its development. Dissections, histological and pathological studies were carried out on the nasal mucosa to understand its function. These results were compared with the horse. Endoscopy showed an external nose with two lips and the upper lip is divided by a groove due to the nasal septum and an obstruction of right nasal cavity was diagnosed in a newborn. Two diverticula (air sacs) were found in the nasal vestibule and an incisive recess (premaxillary sac) in the nasal cavity. These findings were corroborated by 3D reconstructions of the nasal cavities, MRI, anatomical sections and dissections. The presphenoid and ethmoid bones were fused at early stages of fetal development. The ethmoid is the last bone to ossify in the nasal cavity.