bluuebunny/crossref_metadata_2025 huggingface dataset:
Significant association between a non-synonymous snp in IGFBP5 gene and the growth of striped catfish (Pangasianodon hypophthalmus, Sauvage, 1878)
Authors: Tran Trang Thi Huyen , Tran Hoang Son , Le Binh Thi Nguyen , Nguyen Sang Van , Vu Hai-Anh , Kim Oanh Thi Phuong
Published: June 2023

Insulin-like growth factor binding protein 5 (IGFBP5) is the highest conserved member of IGFBP family, and has the broad range of biological activities effecting on the cell growth. This study aims to investigate the association between genetic variation in IGFBP5 gene and the growth of striped catfish (Pangasianodon hypophthalmus). Single nucleotide polymorphisms (SNPs) were discovered and validated in IGFBP5 gene from two growth-selected populations (fast- and slow- growing fish). For SNP discovery, the fragments of IGFBP5 from sample sets of 10 fast- growing fish and 10 slow- growing fish were directly sequenced by Sanger sequencing. In this stage, 4 exonic SNPs were discovered, including a non-synonymous SNP 525 T>A (p. Val16Glu) in exon 1, and three synonymous SNPs (8859 G>A, 11713 C>A, 11992 T>C) in exon 4. The non-synonymous SNP 525 T>A (p.Val16Glu) was filtered to the next step of SNP validation. For validation, the SNP was individually genotyped in the test populations of 70 fast- growing fish and 70 slow- growing fish by single base extension method. Data analysis from the total SNPs which were collected from 80 fast- growing fish and 80 slow- growing fish indicated that non-synonymous SNP 525 T>A (p.Val16Glu) was significantly associated to the growth of striped catfish (p-value <0.001). Analysis of genetic diversity parameters (PIC, MAF) suggested that this SNP is a common variant, contributes significantly to the genetic variance. The non-synonymous SNP 525 T>A (p.Val16Glu) in IGFBP5 gene would become a SNP marker candidate for marker assisted selection (MAS) that can be used in pangasius breeding.
On the nth-order subfractional Brownian motion
Authors: MOHAMED El Omari , MOHAMED Mabdaoui
Published: June 2023

In the present work, we introduce the nth-Order subfractional Brownian motion (S_H^n (t), t ≥ 0) with Hurst index H ∈ (n − 1,n) and order n ≥ 1; then we examine some of its basic properties: self-similarity, long-range dependence, non Markovian nature and semimartingale property. A local law of iterated logarithm for S_H^n (t) is also established.
Value of morphological and laboratory markers in predicting the effectiveness of cryoballoon pulmonary vein isolation in atrial fibrillation
Authors: Kalemberg E. N. , Davtyan K. V. , Blagova O. V. , Brutyan A. A. , Zlobina P. D. , Kharlap M. S.
Published: June 2023

Aim. To determine the clinical, morphological, and biochemical pre­dictors of substrate-specific, non-vein-dependent atrial fibrillation (AF) by morphological and immunological examinations in patients with recurrent AF after pulmonary vein (PV) isolation.

Material and methods. The study included 100 people (58 men and 42 women) with paroxysmal (n=89) and persistent (n=11) AF with a mean age of 58,2±9,4 years. After the primary operation, 15 people withdrew from the study due to refusal of reintervention, and 1 person withdrew due to suppuration of loop recorder bed and its explantation. A total of 100 patients underwent primary cryoballoon PV isolation with myocardial biopsy from the interventricular and interatrial septa, while 60 patients had subsequent hematoxylin-eosin and Van Gieson stains. Also, 100 patients were simultaneously implanted with ECG loop recorders for AF monitoring and blood was taken (before the intervention) for subsequent analysis of inflammation markers and antimyocardial antibodies. Patients underwent clinical observation during the year (visits after 3, 6, 12 months). Patients with relapses were referred for repeat electrophysiological examination and segmental ablation if PV ruptures were detected. After repeated intervention, the follow-up period was extended for a year with the same schedule of visits. According Two groups of patients were formed: without relapses (vein-dependent AF, group I) and with relapses (non-vein-dependent AF, group II), despite the achieved PV isolation as a result of 1 or 2 operations.

Results. An analysis of morphological changes showed that an increase in AF recurrence risk by an average of 6,14 times (95% confidence interval: 1,14-47,6 (p=0,0462)) is associated with stage 2 interventricular septal fibrosis. Lymphocyte infiltration of the interventricular septum was associated with higher AF recurrence by an average of 9 times (95% confidence interval: 0,89-95,37) (p=0,0519).

Among the laboratory parameters, a significantly higher concentration of the N-terminal pro-brain natriuretic peptide and a significantly lower concentration of antimyocardial antibodies were found in the group of patients with recurrent AF after LV isolation (p=0,0101).

Conclusion. Inflammatory myocardium changes are an important link in the pathogenesis of AF and are associated with its recurrence.
Modern ideas about the role of the galaninergic system in the pathogenesis of heart failure and myocardial remodeling
Authors: Yafarova A. A. , Kiselev A. R. , Sheptulina A. F. , Drapkina O. M.
Published: June 2023

Despite advances in diagnosis and treatment, heart failure (HF) still remains one of the unsolved problems of modern cardiology. The appearance and progression of HF is preceded by the development of maladaptive myocardial remodeling. In this regard, the search for new molecules for therapeutic action aimed at preventing myocardial remodeling is of undoubted interest. In this context, the galaninergic system seems to be a promising target. To date, evidence has been accumulated that activation of the galaninergic system leads to an increase in cardiomyocyte protection in the early stages after myocardial injury. It has been shown that galaninergic cascades can influence myocardial remodeling, including cell death, cardiomyocyte hypertrophy, and fibrosis. Various experimental models of heart damage showed that under stress conditions and the action of galanin and its derivatives, carbohydrate metabolism in cardiomyocytes improved, oxidative stress decreased due to antioxidant effect, and hyperproduction of reactive oxygen species by mitochondria decreased. Data were also obtained that galaninergic cascades prevent cardiomycyte hypertrophy by suppressing the expression of some fetal genes. This review article describes the currently studied cellular and molecular effects of galanin on the cardiovascular system, as well as data on the role of the galaninergic system in the pathogenesis of myocardial remodeling.
Comparison of thirty-year survival in the populations of Russia and the United States of America
Authors: Vilkov V. G. , Shalnova S. A. , Kapustina A. V. , Muromtseva G. A. , Drapkina O. M.
Published: June 2023

Aim. To compare survival at a 30-year follow-up in the populations of Russia and USA.

Material and methods. From the Russian studies of 1975-1982, 14728 men and 6140 women aged 18-71 years were included, with follow-up until 2017 (501016 person-years of follow-up). In addition, 2265 men and 2345 women aged 18-71 years from the US population (The Third National Health and Nutrition Examination Survey) were compared with survey data in 1988-1994, with mortality follow-up until 2019 (115523 person-years of follow-up). Kaplan-Meier curves were created in groups of men and women aged 18-29, 30-39, 40-49, 50-59, 60-71 years old. All-cause death was taken into account as an outcome. We analyzed the prevalence of a number of cardiovascular risk factors.

Results. Comparison of Kaplan-Meier curves in Russia and USA shows significant differences in all five age groups of men; in Russia the prognosis was worse (p=0,000). In women, significant differences of the same direction appear only at the age of ≥60 years (p=0,003). Comparison of the prevalence of risk factors showed that in Russia hypertension occurs several times more often than in the United States, while the differences are significant in all age groups of men and women.

Conclusion. At a 30-year follow-up, the prognosis for Russia is worse compared to the United States for all age groups of men and for women >60 years of age.
"Woman — Health Model": Federal Project for health literacy promotion
Authors: Lopatina M. V. , Oussova E. V. , Kontsevaya A. V. , Ipatov P. V. , Drapkina O. M.
Published: June 2023

Состояние здоровья в значительной мере определяет качество жизни человека. Это значимый социально-экономический ресурс и  основа национальной безопасности страны.
Oncolytic Virotherapy: An Advanced Microbial Approach for the Management of Cancer
Authors: Rao Muhammad Farhan Siddiq , Ahmed Bilal , Qadir Muhammad Imran
Published: June 2023

Destruction of the tumor (cancerous) cells may be caused by live viruses, which have replicative ability and replicate selectively in tumor cells, known as oncolytic virotherapy. In comparison of conservative cancer therapy, tumor-selective replicating viruses have more advantages. These viruses have introduced new methodologies for the human cancer treatment. Numerous strategies are used in development of virotherapeutics. Virotherapy is not unusual concept, but modern advances in technology of genetic modification of oncolytic viruses have improved the ability of targeting tumor cells more specifically, it triggered the development of novel ammunition to fight cancer. An effective virotherapeutic
approach with oncolytic viruses exhibits the feasibility and safety under clinical approach. New strategies are being explored to overcome basic obstacles and challenges in virotherapy. Administration of oncolytic viruses, logically, will successfully augment new treatments against many kinds of tumors. Some encouraging antitumor responses shown by combination therapy are provoking strong immunity against established cancer. Chief developments in oncolytic virotherapy have seen in past several years. Significant understandings have been provided by findings on the interface among immune comebacks and viruses, whereas potential results have shown in clinical trials.
CALCULATION OF THE HEAT TRANSFER COEFFICIENT IN THE OUTER BODY FOR A ROTATIONAL DETONATION ENGINE
Authors: VanOsdol John
Published: June 2023

Unsteady heat transfer characterization on the combustion surfaces of rotational detonation engines (RDE) is not well understood. It is generally thought that the complex nature of the unsteady, reacting, compressible fluid flow inside the combustion annulus of the RDE causes the convective heat
transfer coefficient to be significantly higher than it is in other applications. Empirical models that have
been used to analyze this strictly apply to steady flows where dimensionless groups can be employed. Because of the complex flows in RDEs, it is not likely that they will lend themselves to explanation using simple dimensionless parameters. In this work a numerical study is performed where the inside wall surface temperature distribution in the RDE outer body is systematically changed over a given
range that would be characteristic of the startup flows inside an RDE. For each case, temperature distributions inside the outer containment wall of the RDE were calculated and compared with experimental data. The closest match was then used to directly calculate the convective heat transfer
coefficient on the inside surface of the RDE. The calculation results show that the local heat transfer
coefficient CH is lower than what has been commonly used for these flows. The results show that CH is within the range of normal forced convection of a gas.
Particle-scale simulations of the compression and shearing of kaolin clay
Authors: de Bono John P. , McDowell Glenn R.
Published: June 2023

This paper presents a new model for particle-scale simulations of clay. Using the discrete-element method, featuring realistically shaped platelets with separate interactions between the various platelet surfaces, simulations of isotropic normal compression and triaxial shearing are performed. A normal compression line is established, and for the first time so is a critical state line, which is parallel to the normal compression line in e–log p space. The critical state line is obtained from conventional, constant mean stress and constant volume tests on normally consolidated and overconsolidated samples. The critical state line appears linear in q–p space and the first insights into a state boundary surface for the clay are established. This new model appears capable of capturing the key features of macroscopic clay behaviour.
Sezai Karakoç, T.S. Eliot ve Abdullah Et-Tayyib’in Eserlerinde Medeniyet ve Kültür İlişkisi
Authors: ABDALLAH Suliman Nasir İbrahim
Published: June 2023

Sezai Karakoç modern Türk edebiyatı, düşüncesi ve şiiri üzerinde büyük etkisi olan

bir şair-düşünür, Thomas Stearns Eliot modern Amerikan-İngiliz şiirinde geniş yankı bırakan,

dönemin müstesna şair-düşünürlerinden biri ve Abdullah et-Tayyib Sudan ve Arap

edebiyatında oldukça etkili olan ve özel yere sahip şair-düşünürlerden biridir. Türk, İngiliz

ve Sudan edebiyatının önde gelen isimlerinden olan bu üç büyük ismin eserlerinde,

medeniyet ve kültür ilişkisi bazı görüş farklılıklarına rağmen ortak bir perspektif olarak

tezahür etmektedir. Bu çalışmada üç önemli şair-düşünürün medeniyet ve kültür kavramlarını

eserlerine nasıl yansıttıklarını karşılaştırmalı edebiyat bağlamında irdelemek ve

değerlendirmek istiyoruz. Çünkü bu üç büyük ismin bu kavramlara ilişkin tanımları ve

açıklamaları, kültür ve medeniyet ilişkisine ışık tutmaktadır. Yaptığımız çalışmada, Sezai

Karakoç’a göre medeniyetin kültürü de içerdiği; Thomas Stearns Eliot’a göre bu iki kavramın

birbirinden ayrılamaz olduğu ve iç içe kullanıldığı; Abdullah et-Tayyib’de ise bu

iki kavramı zaman zaman birbirinin yerine kullanmakla birlikte kültürün, onun eserlerine

bir medeniyet unsuru olarak yansıtıldığı tespit ve izah edilmektedir.
Prosodic generalizations in the Brazilian Portuguese diphthongal plural
Authors: Becker Michael , Becker Michael , Nevins Andrew , Sandalo Filomena , Rizzato Érick
Published: June 2023

The diphthongal plural in Brazilian Portuguese is sensitive to three phonological factors: monosyllabicity, licensing of nasal diphthongs by stress, and vertical dispersion of oral diphthongs. We present an analysis that captures the gradient distribution of the Brazilian Portuguese plurals ending in diphthongs, both oral and nasal, using a probabilistic grammar based on the well-understood factors mentioned above. This grammar is trained on corpus data and is sensitive to prosodic generalizations; it correctly derives existing plurals while accurately generalizing beyond individual lexical items and predicting participants’ choices in nonce word tasks. The results are incompatible with analyses that apply uniformly to all lexical items, as well as with those that simply memorize semipredictable plurals without incorporating phonologically-based generalizations.
Praskovya Semenovna Vorobieva-Vladimirova – Ekaterinodar journalist and critic of A. M. Remizov
Authors:   Irkutsk State University, Vakhnenko Ekaterina E.
Published: June 2023

The paper explores the assessment of Alexey Remizov’s works of the 1910s by a journalist and critic, Praskovya Semenovna Vorobieva-Vladimirova. Some analytical articles about the writer’s work published on the pages of the Ekaterinodar newspaper “The Kuban Courier” have been identified and analyzed. The specifics of the perception of Remizov’s artistic heritage in the context of the editorial policy of this newspaper is considered in brief reports, notes, and review articles devoted to the latest works of Russian literature. The paper also describes the approaches and interests of the correspondents of “The Kuban Courier” in covering the features of the modern literary process. Attention is also paid to the professional interests of Vorobieva-Vladimirova as a leading collaborator, observer, and critic of Central Russia magazines. The analysis of a collection of letters for the first time introduced into scholarly circulation revealed the specifics of early personal and creative contacts between Vorobieva-Vladimirova and Remizov. A significant part of the letters reveals the interest in the literary personality and legacy of Remizov. Also, they unveil the attempts of a provincial correspondent already recognized in professional circles and known among readers in southern Russia to enter the St. Petersburg world of the daily press and establish creative contacts with writers. Such cooperation between a writer and a critic based on meaningful epistolary dialogue is a rare example in the history of literary relations.
“The ancient duel of Mstislav…”: the story of “The Russian Primary Chronicle” in a V. Sosnora’s poem “The battle of Mstislav with Rededya” (1959)
Authors:   Institute of Philology of the Siberian Branch of the Russian Academy of Sciences, Kovaleva Tatyana I. , Loshchilov Igor E. ,   Institute of Philology of the Siberian Branch of the Russian Academy of Sciences
Published: June 2023

This paper deals with the poem by V. Sosnora, “The Battle of Mstislav with Rededya” (1959). The poem is based on the story of “The Russian Primary Chronicl” under 6530 (1022) devoted to the event mentioned in the title. According to the poet, when writing his “historical” poems, he “re-imagines” the material of ancient Russian monuments and brings it closer to the modern reader. The poet’s creative method was often misunderstood by critics and satirists who referred to his “irreverent” treatment of history. In terms of literary criticism, “re-imagining” implies using a rich palette of poetic means of allusions and associations, a deliberate mixture of ancient and modern, and others. Addressing the named story of “The Russian Primary Chronicl,” as well as to other chronicle stories and “The Song of Igor’s Campaign,” Sosnora creates a poetical plot. This plot emphasizes the dynamics of the duel of chronicle characters, marks individual features of their appearance, hides by default an important detail for the chronicle narrative (knife), and introduces a dialogue (an expressive squabble before the battle of the title characters), reworking the chronicle event to give the battle scene “visual”, “cinematic” effect. This could not be the case in Old Russian narration subordinated to literary etiquette. By arranging the semantic and rhythmic accents under his idea, the poet seems to offer a fantastic reconstruction of one of the Boyan’s songs.
Communicative variation of the typical existential syntactic structure in the Surgut dialect of Khanty
Authors:   Institute of Philology of the Siberian Branch of the Russian Academy of Sciences, Plotnikov Ilya M.
Published: June 2023

This paper considers the communicative variation in the Surgut dialect of Khanty, one of the indigenous languages of Siberia. A typical existential syntactic structure comprises three elements, to be referred to here as “subject,” “localizer,” and “predicate.” The prototypical meaning of this structure can be represented by simple existential sentences. The analysis covers 200 realizations of this structure sampled from a collection of texts representing different genres and idioms of the Surgut dialect. Four main variants of the structure are identified, taking into account both the constituent order and intonation structure. The realizations are grouped under the context in which they are used and their function in the text. These groups represent several distinct communicative situations in which the structure concerned is commonly used: discovering an object when observing a visible area, establishing the time, place, and main characters at the beginning of a text, switching attention to a new object, and others. These situations are grouped into three main situations determined by the position of the main focus on the subject, localizer, or predicate. Each main situation is associated with a specific communicative variant, in which the focal element is placed in such a way as to be emphasized intonationally. The realizations with pronominal adverbs in the localizer position are considered separately. It is in these cases that the focal subject causes two communicative variants that appear to be interchangeable, as no direct correlation between these communicative variants and the types of communicative situations has been found.
Agricultural climate security: Threats and challenges to adaptation
Authors:   Russian Presidential Academy of National Economy and Public Administration, Yakovleva Elena N. , Kryukova Irina V. ,   Vologda State Dairy Farming Academy
Published: June 2023

Global climate change poses two major challenges to the agricultural sector of the economy. Firstly, the importance and extent of the sector’s impact on greenhouse gas pollution needs to be assessed, and, based on this, priorities and mitigation strategy should be identified. Secondly, sustainable agricultural development needs to be ensured by adapting to the threats and chances posed by global climate change. Therefore, the aim of this study was to develop recommendations for ensuring the climate security of the agricultural sector through the disclosure of these two aspects. Based on the review of scientific literature, the conceptual and applied problems of the implementation of climate policy in the agricultural sector of the economy were summarized. The national contribution of agriculture to greenhouse gas pollution was studied on the example of the Russian Federation using the authors’ methodology. The problems were classified, the negative and positive consequences of global warming for the agricultural sector and their corresponding ways of adaptation were summarized. Priority directions of the state policy for ensuring the climate security of agriculture were developed.
The interconnection between subjective well-being and household decisions to invest in risky financial assets: The experience of China
Authors:   National Research Tomsk State University, Kefeng Yuan ,   Pedagogical Ningde University of Science, Nedospasova Olga P. ,   Tomsk State University
Published: June 2023

The authors decided to investigate the relationship between a person’s subjective idea of well-being and some aspects of their economic behavior, in particular, participation in risky forms of investment. The results of the Chinese Household Social Survey 2017 (CGSS-2017) were used as the data source. The results showed that subjective assessments of well-being are positively correlated with (1) the likelihood of households participating in risky financial investments, (2) the proportion of households making risky financial investments. The authors also found that the age of respondents, their marital status, total family income, health status, housing conditions, participation in voluntary medical and pension insurance programs correlate with a person’s subjective idea of well-being and, as a result, affect the propensity and scale of investment of family budget into risky financial assets.
Share digitalization as a source of cooperation financing
Authors:   Center for Continuing Professional Education Novokurs LLC, Ali-Askiari Sarvaz A. , Maksudov Khofizzhon Z. ,   Center for Continuing Professional Education Novokurs LLC
Published: June 2023

The novelty of the study lies in the proposal of a new approach to placing a part of the mutual fund in centralized digital currency markets. The aim of the article is to propose a mechanism and model for the digitalization of the share through the utilitarian digital rights of the system of consumer and agricultural cooperation. The study makes it possible to evaluate the mechanism of the new approach to involving the urban and rural population in participating in the country’s economy as a shareholder. The allocation of part of the share fund to associates imposes additional fiscal and financial control on the part of state and other independent structures. Control allows one to reduce the risk of losing a share, which will lead to an increase in the number of shareholders and affect the efficiency of cooperative management. Participants in the operations of the digital system additionally offer interested parties agreements to attract shareholders and to promote investment. The study used general scientific methods, methods of structural and logical analysis. The theoretical and methodological foundations of the study were the works of modern scientists and the current legal documents. The presented approach can be applied at enterprises of the cooperative system. The results of the study are recommended for use by non-profit institutions and organizations, corporate organizations, in the field of attracting shareholders from the urban environment for the development of the territory and the rational management of income and assets.
Uncovering Students’ Genuine Misconceptions: Evidence to Inform the Teaching of Chemical Kinetics
Authors: Habiddin Habiddin , Page Elizabeth Mary
Published: June 2023

The aim of this study is to investigate first-year university students’ misconceptions in chemical kinetics by analysing data obtained from the four-tier diagnostic instrument of chemical kinetics (FTDICK). 335 first-year chemistry students from two Indonesian and one UK universities participated in this study. The procedure described here is the first of its type to ensure those misconceptions are genuine. Numerous genuine misconceptions within chemical kinetics were revealed among first-year chemistry undergraduates. Although many of the misconceptions found here concur with those results previously published using other instruments, some novel findings were uncovered. These misconceptions can be attributed to a variety of factors including mathematical weakness, carelessness and difficulty in interpreting and extracting information from diagrams, graphs and other non-textual information. On the basis of the results from this study we make some recommendations for improving the effectiveness of chemical kinetics’ teaching at this level.
Design, Development and Optimization of Carmustine- Loaded Freeze-Dried Nanoliposomal Formulation Using 32 Factorial Design Approach
Authors: Honmane Sandip M. , Charde Manoj S. , Osmani Riyaz Ali M.
Published: June 2023

The objective of the current study was to develop and optimize a novel lyophilized liposomal formulation of anticancer agent carmustine, or bis-chloroethyl nitrosourea (BCNU) for prolonged release that could overcome the dose-dependent side effects and improve its bioavailability at the site of action. The optimization was done using a 32 factorial design approach wherein soya phosphatidylcholine (SPC) and cholesterol (CH) as independent variables. The optimized formulation (F4) exhibited high entrapment efficiency (81.57%) with an average vesicle size of 141.7 nm and a −22.6 mV Zeta potential. In-vitro drug release studies from all formulations revealed that the BCNU was released for up to 36 hours following the Higuchi matrix release model. The TEM, FTIR, DSC, PXRD, and SEM analyses confirm the formation of liposomes. BCNU-loaded nanoliposomal formulation demonstrated prolonged release, suggesting that it could be used to supplement cancer therapy efficiently with a reduction in dose-dependent side effects.
Synthesis, SC XRD Based Structure Elucidation, Supramolecular Assembly Exploration Via Hirshfeld Surface Analysis, Computational and QTAIM Study of Functionalized Anilide
Authors: Tahir Muhammad Nawaz , Ashfaq Muhammad , Ali Akbar , Lai Chin Hung , Rao Bojja Rajeshwar , Munawar Khurram Shahzad , Shahid Irshad Ali
Published: June 2023

The anilide compound named (Z)-4-(2-methoxy-4-nitrophenyl)amino)-4-oxobut-2-enoic acid (MAOA) has been synthesized by the chemical reaction of 2-methoxy-4-nitroaniline and maleic anhydride in ethyl acetate. The synthesized compound was characterized by elemental analysis, FT-IR and UV-Vis spectroscopy, and TGA/DSC technique. Furthermore, the crystal structure was analyzed by the single crystal X-ray diffraction (SC XRD) technique. The supramolecular assembly of MAOA in terms of non-covalent interactions was explored by Hirshfeld surface analysis. Void analysis inferred that MAOA is expected to have good mechanical properties. The crystal packing environment was further investigated by interaction energy between molecular pairs and energy frameworks. Moreover, the result of the gas-phase DFT study showed that there is an intramolecular N–H···O and O–H···O hydrogen bond in MAOA due to the distance between D and A being smaller than the sum of their van der Waals radii. The result of the QTAIM study showed that there should also be an intramolecular CH···O hydrogen bond with a strength of 3.40 kcal/mol in MAOA.
Library Aligner v1
Authors: not provided ckremitz
Published: June 2023

Protocol on how to run Library Aligner in FIVTools once sequencing data has been returned to us from the Sequencing Core. It also has an option for checking the Representation of a gRNA library.
Striatal Cannula Window Implantation v1
Authors: Nagappan Shivathmihai
Published: June 2023

This protocol describes how to implant a cannula window over the dorsolateral striatum in mice to enable two-photon imaging of dopamine axons in the dorsal striatum.
FIVTools Thresholding and Measures v1
Authors: not provided d.grigore
Published: June 2023

YouTube: FIVTools Thresholding and Measures Walkthrough The goal is to set a criteria on a single FOV for machine learning that will ultimately lead to a final output of single cells with associated intracellular metrics.
iPSC to Motor Neuron Differentiation Various Protocols v1
Authors: not provided jwaligor
Published: June 2023

iPSC to Motor Neuron Differentiation Various Protocols
TIMSTOF Compass 4.0 Methods - LCMS and MALDI - OrsburnLab 2023 v1
Authors: Orsburn Benjamin
Published: June 2023

The methods included here are in support of research submissions from the www.OrsburnLab.org group in 2023 from a TIMSTOF Flex system with EasyNLC1200, EvoSep One and MALDI (1) front end sources. Methods include: pasefRIQ used for SCoPE-MS (DefaultTIMSTOF40_TMT_highsensitivity_pasefSCOPE diaPASEF methods optimized for the EvoSep One - diaPASEF-shortgradient-1800V the same - optimized for EasyNLC 1200 using a 10um emitter - TIMSTOF_40_shortgradient_DIA ddaPASEF method for normal high input (100ng - 500ng proteomics loads) -DefaultDDA_TIMST40_5_6_2023 ddaPASEF methods optimized for the EvoSep One Whisper for ultralow input immunoenriched samples - DefaultDDA_TIMS40_high_sensitivity A MALDI-MS method for TIMSTOF Flex to test the effectiveness of direct transfer of MALDI instrument methods between different TIMSTOF imaging mass spectrometers: 20220925_SCZ_GelMethod_AngelLab_transfer.m
Possibilities of early diagnosis of localized prostate cancer in outpatient practice of a urologist at the regional level in the stavropol territory
Authors: Panchenko I. A. , Marabyan E. S. , Garmash O. N. , Panchenko R. I. , Zargarov V. O. , Verigin V. V.
Published: June 2023

Aim. To develop and implement an algorithm for early diagnosis of a localized form of prostate cancer based on the determination of the specificity of the prostate-specific antigen (PSA) marker in different age groups.

Materials and methods. In the framework of a screening program for early detection of diseases of the urogenital system developed at the Stavropol Regional Clinical Specialized Uroandrological Center, middle-aged and elderly men were examined using the following characteristics: IPSS (International Prostate Symptom Score), serum PSA level, digital rectal exam, transrectal prostate ultrasound, urodynamic tests, as well as transrectal ultrasound-guided prostate biopsy in cases of elevated total PSA. The data (for 2010–2021) on the frequency of detection of localized prostate cancer depending on patient age (40–59 years, 60–69 years, ³70 years) and total PSA level were analyzed.

Results. With an increase in total blood PSA >4 ng/ml, there is a clear trend towards a decrease in the detection of benign prostatic hyperplasia and an increase in the detection of adenocarcinoma, which is confirmed by the data of histological examinations of biopsy specimens. In 81 % of cases, transrectal multifocal biopsy of the prostate gland (adenocarcinoma and prostatic intraepithelial neoplasia was detected) under transrectal ultrasound control is justified, including when PSA values are in the “gray zone”, which shows a rather high value of this method.

Conclusion. Total serum PSA level is a readily available indicator for differential diagnosis of prostate diseases including detection of localized prostate cancer in out-patient conditions. The obtained data points at high specificity of this marker in men over 40 years old. Measurement of serum PSA level at the age of 40–49 years is necessary for early detection of oncological pathology. For total serum PSA level >4 ng/mL, mandatory transrectal multifocal prostate biopsy is recommended at any age.
Combined use of percutaneous and endovascular surgery in the diagnosis and treatment of arteriovenous malformation of the kidneys. Clinical observation
Authors: Saakyan A. A. , Kadyrov Z. A. , Gegenava B. B. , Fatikhov R. R. , Suleymanov S. I. , Faniev M. V.
Published: June 2023

This article describes the causes of vascular abnormality of the upper urinary tract, arteriovenous malformation of the kidneys, its variants, possible clinical manifestations, methods of diagnosis of this condition, as well as ways to eliminate hemorrhagic complications caused by this pathology. The main method of correction of arteriovenous malformation is superselective embolization of vessels of the malformation zone. This clinical example presents a rare case of surgery in which embolization was unacceptable due to technical difficulties associated with the complexity of vascular angioarchitectonics, with the impossibility of superselective entry into varix through the arterial channel. In this regard, endovascular surgeons and urologists had to use a combined approach – percutaneous varix puncture with the introduction of a spiral and an embolizing substance through a puncture needle under the control of selective angiography of the branches of the renal artery.
Typical errors in the diagnosis and treatment of testicular torsion in adults. Clinical observation
Authors: Faniev M. V. , Suleymanov S. I. , Kadyrov Z. A. , Уagudaev D. M. , Giyoev M. A.
Published: June 2023

Testicular torsion refers to diseases of the acute scrotum group and requires early diagnosis and emergency surgical care. Despite the characteristic clinical signs, sometimes there are difficulties in diagnosis, which leads to delayed treatment and irreversible damage to the testicle.

The article describes a clinical case of testicular torsion in a young patient with errors in diagnosis, which led to the removal of the testicle. As a result of a diagnostic error – an incorrect collected anamnesis and not timely execution of ultrasound dopplerography, conservative treatment for epididymoorchitis was carried out. The given example illustrates the importance of a thorough study of the anamnesis of the disease – the onset and nature of pain, nausea, as well as local symptoms that are not characteristic of other acute testicular diseases. In addition, if a testicular torsion is suspected, it is necessary to perform urgent, sometimes repeated ultrasound dopplerography of the scrotum organs, which is of irreplaceable value for solving the issue of emergency surgical treatment. At the slightest suspicion of testicular torsion, in particular the presence of several characteristic symptoms and ultrasound data, you need to resort to emergency surgery.
The negative impact of phthalates on male reproductive system and fertility
Authors: Sedova A. O. , Сhernykh V. B.
Published: June 2023

The paper overviews the negative impact of phthalates on the male reproductive system, spermatogenesis, semen parameters and male fertility. The analysis of the literature revealed few studies devoted to the study of the effect of phthalates on reproductive health, gametogenesis and fertility in humans and laboratory models (rats, mice). Although epidemiological studies on the effect of phthalates on the male reproductive health are not big, some toxicological studies show that some phthalates are potential reprotoxicants.
Is oxytocin an undeservedly forgotten hormone in men?
Authors: Tyuzikov I. A. , Grekov E. A. , Smirnov A. V.
Published: June 2023

Oxytocin is one of the most ancient mammalian hormones in terms of phylogeny. Over the more than 100-year history of his scientific study, views on the biological functions of oxytocin in humans have undergone a significant and even revolutionary changes. For a long time, this neurohypophysis hormone, which is formed in the hypothalamus and is only deposited in it (central synthesis), was associated exclusively with female reproduction in the aspect of labor, breastfeeding and maternal behavior. However, over the past decades, a unique and extensive scientific base has been created confirming the universal nature of the effects of oxytocin in both sexes, which is predetermined by the presence of additional peripheral sites of oxytocin secretion in men and women, as well as an extensive receptor apparatus in many cells and tissues, which together allows oxytocin to function both endocrine central and auto- and paracrine local signaling pathways. This fact convincingly proves that oxytocin has a much wider range of physiological effects in the body of both sexes, and the results of modern research allow us to consider it as a central neuropeptide and a peripheral multifunctional hormone of great biological and social significance for humans.

The review article briefly discusses the endocrinology of oxytocin and in more detail its physiological effects in men, including its role in social and sexual behavior, erectile function, protection from stress, reproduction, metabolism, prostate gland.
Anorexia nervosa through the lens of primary health care practitioners in the Kyrgyz Republic
Authors: Moldokulova Zharkyn , Molchanova Elena S. , Kosterina Elena V.
Published: June 2023

BACKGROUND: Anorexia nervosa was investigated in a large number of studies. Several of the studies in our review highlighted how important it is to factor in the opinion of health care practitioners if we want to improve the quality of the medical care provided to patients with anorexia nervosa. Additionally, the dominance of studies focused on anorexia nervosa within the Western culture raised the need for cultural diversity in such research.
AIM: The present study endeavored to explore how primary healthcare practitioners in Bishkek, Kyrgyz Republic, perceive anorexia nervosa and people who suffer from it.
METHODS: The study used the qualitative approach of Interpretative Phenomenological Analysis. Six semi-structured interviews with six primary health care practitioners from Bishkek were conducted.
RESULTS: The analysis of the collected data pointed towards four themes: (1) Anorexia nervosa as a reaction to distressing events in the form of loss of appetite; (2) Attributing blame for developing anorexia nervosa by a patient to family members; (3) Local versus Western socio-cultural standards in the development of anorexia nervosa and seeking treatment; and (4) Denial of mental health disorders by patients.
CONCLUSION: The discussion of the interpreted results led us to the conclusion that the perceptions of the health care providers in Bishkek represent the common prism through which anorexia nervosa is viewed in the larger population, which is constructed out of information received from the physical and virtual environments.
New approaches to the treatment of rosacea on the basis pathogenesis factors and clinical forms
Authors: Yakovleva Anna O. , Verbenko Dmitry A. , Kondrakhina Irina N. , Plakhova Ksenia  I. , Kubanov Alexey A
Published: June 2023

Uncovering the molecular mechanisms of the rosacea pathogenesis and treatment are still of great importance. The review encompasses the modern knowledge of rosacea classification, characterization of the severity of the course of the subtypes, and pathogenesis features. Recent methods of rosacea treatment are presented, including the use of botulinum neuroprotein for the correction of the erythematous-telangiectatic subtype of the dermatosis.
The relevance of this problem is due to the chronic course of the disease and the peculiarity of the localization of rosacea manifestations that leads to a traumatic effect and often causes social exclusion of patients.
The rise of the rosacea incidences, along with other reasons like characteristics of the course of dermatosis, accompanied by frequent long-term relapses, is due to the resistance to generally accepted methods of drug therapy, often resulting in shortened clinical remission. All the factors lead to decreasing of patients life quality that require the search for effective therapy approaches. Among modern rosacea therapy, the application of botulinum neuroprotein is widely used; however, the clinical studies confirming the effectiveness of this technique are not presented enough.
Fulminant acne induced by simultaneous taking of isotretinoin and anabolic steroids
Authors: Smirnova Irina O. , Sukhenko Alla V. , Petunova Yanina G. , Shin Natalia V. , Khazhomiya Kristina D.
Published: June 2023

Acne fulminans is a rare disease characterized by the sudden appearance of painful nodules with a pronounced tendency to ulceration, accompanied by general symptoms, including fever, arthralgia, hepatomegaly and impaired blood parameters. Among its most frequent triggers are anabolic steroids and isotretinoin.
The article describes a patient whose disease was caused by the sequential administration of anabolic steroids and systemic isotretinoin. The clinical picture of acne fulminans in the patient was represented by typical skin rashes, changes in blood parameters, arthralgia and myalgia, as well as nodular erythema. Systemic antibacterial therapy with doxycycline was ineffective. The patient was hospitalized in the Saint Petersburg State Budgetary Healthcare Institution "City Dermatovenerological Dispensary", where he was treated with prednisone and then with systemic isotretinoin. Due to the severe course of the process, the cumulative dose of isotretinoin achieved 240 mg/kg. Against the background of treatment, studies of aspartate aminotransferases, alanine aminotransferases, bilirubin, cholesterol, triglyceride indicators were regularly conducted, the values of which did not exceed the limits of reference indicators. The remission of the disease was 3 years, there are residual phenomena in the form of scars. Scar correction is carried out using a fractional CO2 laser (SmartXide Touch), no more than 3% of the skin is treated in one procedure.
The peculiarity of the observation presented by us is the association of fulminant acne with simultaneous administration of steroid hormones and isotretinoin, the combination of fulminant acne with erythema nodosum, as well as good tolerance of high doses of isotretinoin.
The literature review discusses data on etiopathogenesis, features of clinical manifestations, approaches to the treatment of fulminant acne. Modern approaches to the classification of the disease are also presented.
Justification of the efficacy of combined therapy with dupilumab and cyclosporine in children with severe dermato-respiratory syndrome
Authors: Aoun Rami Yu. , Udzhukhu Vladislav Yu. , Kukalo Svetlana V. , Batkaev Edgem A.
Published: June 2023

BACKGROUND: Atopic dermatitis is characterized by a clear upward trend in the prevalence and development of severe forms of dermatosis, which represents an important medical and social problem.
AIM: Pathogenetic justification and study of clinical efficacy of combined use of dupilumab and cyclosporine in children with severe refractory atopic dermatitis.
MATERIALS AND METHODS: The study included 30 children and adolescents over 6 years of age, with a diagnosis of atopic dermatitis with severe disease severity. The control group consisted of 15 people. EASI, Pruritus VAS score, CDLQI index were used to assess the dynamics of disease symptoms. The content of interleukins in blood serum was determined by enzyme immunoassay. Children included in the study were divided into two groups of 15 patients by randomization. Group 1 patients received dupilumab monotherapy, Group 2 patients were treated with a combination of dupilumab and cyclosporine.
RESULTS: Positive dynamics in the course of the cutaneous pathological process was observed in all patients. A more pronounced and persistent tendency to further decrease of EASI values was preserved in patients receiving combined therapy. During dupilumab monotherapy and combined treatment there was a significant reduction in nasal obstruction, a decrease in the number of bronchial obstruction/cough attacks. In the course of therapy in all patients it was possible to achieve a significant reduction in the negative impact of atopic dermatitis on the quality of life of children and adolescents. Dupilumab therapy contributed to normalization of cytokine status, eosinophil levels and total immunoglobulin E. However, after 12 weeks of dupilumab monotherapy, signs of Th1-response activation were revealed.
CONCLUSION: Combined therapy reduces the duration of treatment with dupilumab to 12 weeks, avoids side effects and complications, and significantly increases clinical efficacy of therapeutic measures in children and adolescents with severe atopic dermatitis.
What do we know about isotretinoin?
Authors: Olisova Olga  Yu. , Shepeleva Anastasia V.
Published: June 2023

Acne treatment has been revolutionized since 1982 when isotretinoin was approved for the first time for the condition.
Isotretinoin (13-cis-retinoic acid) is still the most effective treatment for acne. Systemic retinoid (a derivative of vitamin A) is the only drug acting on all links of the pathogenesis of this disease. Isotretinoin is believed to improve the condition of the skin in acne by reducing sebum production by the sebaceous glands, normalizing follicular keratinization and suppressing the growth of Actinobacterium acnes (Propionibacteriaceae family) by changing the follicular environment and reducing inflammation.
Despite being widely prescribed by doctors for almost forty years, there is still a lot of uncertainty about how best to prescribe this drug, how long the remission lasts, and what side effects there may be. Isotretinoin has been proven to be highly effective and well tolerated, but like any medicine, it also has certain side effects, but it should be understood that they dose-dependent and reversible.
We provide up-to-date information on the possible side effects of isotretinoin and discuss ways to prevent and treat these side effects.