bluuebunny/crossref_metadata_2025 huggingface dataset:
HER2-Low Status Is Not Accurate in Breast Cancer Core Needle Biopsy Samples: An Analysis of 5610 Consecutive Patients
Authors: Lu Yujie , Zhu Siji , Tong Yiwei , Fei Xiaochun , Jiang Wu , Shen Kunwei , Chen Xiaosong
Published: December 2022

Background: HER2-Low status is found in approximately half of breast cancer patients and shows potential benefits from novel antibody–drug conjugates (ADCs). Data on the accuracy of HER2-Low status between core needle biopsy (CNB) and surgical excision specimen (SES) samples are lacking. We aimed to investigate the accuracy of HER2-Low status diagnosis between CNB and SES samples. Methods: Consecutive early-stage breast cancer patients who underwent surgery from January 2009 to March 2022 with paired CNB and SES samples were retrospectively reviewed. HER2-Low was defined as IHC 1+ or IHC2+ and FISH-negative. Concordance rates were analyzed by the Kappa test. Further clinicopathological characteristics were compared among different HER2 status and their changes. Results: A total of 5610 patients were included, of whom 3209 (57.2%) and 3320 (59.2%) had HER2-Low status in CNB and SES samples, respectively. The concordance rate of HER2 status in the whole population was 82.37% (Kappa = 0.684, p < 0.001), and was 76.87% in the HER2-Negative patients (Kappa = 0.372, p < 0.001). Among 1066 HER2-0 cases by CNB, 530 patients were classified as HER2-Low tumors. On the contrary, in 3209 patients with HER2-Low tumor by CNB, 387 were scored as HER2-0 on the SES samples. ER-negative or Ki67 high expression tumor by CNB had a high concordance rate of HER2-Low status. Conclusions: A relatively low concordance rate was found when evaluating HER2-Low status between CNB and SES samples in HER2-Negative breast cancer patients, indicating the necessity of retesting HER2 low status at surgery, which may guide further therapy in the era of anti-HER2 ADCs.
Measurable Residual Disease Monitoring by Locked Nucleic Acid Quantitative Real-Time PCR Assay for IDH1/2 Mutation in Adult AML
Authors: Kao Hsiao-Wen , Kuo Ming-Chung , Huang Ying-Jung , Chang Hung , Hu Shu-Fen , Huang Chein-Fuang , Hung Yu-Shin , Lin Tung-Liang , Ou Che-Wei , Lien Ming-Yu , Wu Jin-Hou , Chen Chih-Cheng , Shih Lee-Yung
Published: December 2022

Locked nucleic acid quantitative Real-Time PCR (LNA-qPCR) for IDH1/2 mutations in AML measurable residual disease (MRD) detection is rarely reported. LNA-qPCR was applied to quantify IDH1/2 mutants MRD kinetics in bone marrow from 88 IDH1/2-mutated AML patients, and correlated with NPM1-MRD, clinical characteristics, and outcomes. The median normalized copy number (NCN) of IDH1/2 mutants decreased significantly from 53,228 (range 87–980,686)/ALB × 106 at diagnosis to 773 (range 1.5–103,600)/ALB × 106 at first complete remission (CR). IDH1/2 LNA-qPCR MRD was concordant with remission status or NPM1-MRD in 79.5% (70/88) of patients. Younger patients and patients with FLT3 mutations had higher concordance. The Spearman correlation coefficient (rs) and concordance rate between the log reduction of IDH1/2 LNA-qPCR and NPM1-MRD were 0.68 and 81% (K = 0.63, 95% CI 0.50–0.74), respectively. IDH1/2-MRD > 2 log reduction at first CR predicted significantly better relapse-free survival (3-year RFS rates 52.9% vs. 31.9%, p = 0.007) and cumulative incidence of relapse (3-year CIR rates 44.5% vs. 64.5%, p = 0.012) compared to IDH1/2-MRD ≤ 2 log reduction. IDH1/2-MRD > 2 log reduction during consolidation is also associated with a significantly lower CIR rate than IDH1/2-MRD ≤ 2 log reduction (3-year CIR rates 42.3% vs. 68.8%, p = 0.019). LNA-qPCR for IDH1/2 mutation is a potential MRD technique to predict relapse in IDH1/2-mutated AML patients, especially for those with IDH1/2 MRD > 2 log reduction at first CR or a concurrent FLT3 mutation.
Association between Intratumoral CD8+ T Cells with FoxP3+ and CD163+ Cells: A Potential Immune Intrinsic Negative Feedback Mechanism for Acquired Immune Resistance
Authors: Fortis Sotirios P. , Sofopoulos Michael , Goulielmaki Maria , Arnogiannaki Niki , Ardavanis Alexandros , Perez Sonia A. , Gritzapis Angelos D. , Baxevanis Constantin N.
Published: December 2022

Acquired immune resistance (AIR) describes a situation in which cancer patients who initially responded clinically to immunotherapies, after a certain period of time, progress with their disease. Considering that AIR represents a feedback response of the tumor against the immune attack generated during the course of immunotherapies, it is conceivable that AIR may also occur before treatment initiation as a mechanism to escape endogenous adaptive antitumor immunity (EAAI). In the present study, we assessed the EAAI in paraffin-embedded breast primary tumor tissue samples and drew correlations with the clinical outcomes. In particular, we analyzed densities of CD8+ cells as elements mediating antitumor cytotoxicity, and of CD163+ and FoxP3+ cells as suppressor elements. We found a direct correlation between the densities of CD8+ cells and of CD163+ and/or FoxP3+ cells in the vast majority of patients’ tumors. Importantly, the vast majority of patients whose tumors were overpopulated by CD8+ cells developed AIR, which was characterized by high intratumoral CD163+ and/or FoxP3+ cell densities and reduced overall survival (OS). We also showed that AIR depends on the levels of CD8+ cell-ratios in the tumor center to the invasive margin. Our data suggest that tumors develop AIR only when under a robust endogenous immune pressure.
Progestin Resistance and Corresponding Management of Abnormal Endometrial Hyperplasia and Endometrial Carcinoma
Authors: Lv Mu , Chen Peiqin , Bai Mingzhu , Huang Yan , Li Linxia , Feng Youji , Liao Hong , Zheng Wenxin , Chen Xiaojun , Zhang Zhenbo
Published: December 2022

With a younger tendency in morbidity age, endometrial cancer (EC) incidence has grown year after year. Worse, even more commonly occurring is endometrial hyperplasia (EH), which is a precancerous endometrial proliferation. For young women with early EC and EH who want to preserve fertility, progestin therapy has been utilized as a routine fertility-preserving treatment approach. Nevertheless, progestin medication failure in some patients is mostly due to progestin resistance and side effects. In order to further analyze the potential mechanisms of progestin resistance in EH and EC, to provide theoretical support for effective therapeutic strategies, and to lay the groundwork for searching novel treatment approaches, this article reviews the current therapeutic effects of progestin in EH and EC, as well as the mechanisms and molecular biomarkers of progestin resistance, and systematically expounds on the potential therapeutic methods to overcome progestin resistance.
A New Species of the Genus Robertgurneya Apostolov & Marinov, 1988 (Copepoda: Harpacticoida: Miraciidae) from a Sublittoral Zone of Jeju Island, Korea
Authors: Yeom Jisu , Lee Wonchoel
Published: December 2022

A new species, Robertgurneya jejuensis sp. nov., was described from sandy sediment samples collected at a depth of 25 m on Mun Island, Jeju, in June 2018. The new species is morphologically similar to Robertgurneya similis similis (Scott A., 1896) and Robertgurneya donghaensis Bang, 2021; this is the second record of the genus Robertgurneya in South Korea. The morphological characteristics of the similis group within the genus Robertgurneya, to which the new species is ascribed, are summarized here. Furthermore, an identification key is provided based on the summary. Molecular identification of the collected specimens, based on the nuclear 18S ribosomal RNA (18S rRNA) and the mitochondrial cytochrome oxidase subunit I fragment, was obtained. Finally, a phylogenetic tree was constructed to present the position of Robertgurneya within the Miraciidae family based on 18S rRNA sequences which is relatively conserved. As a result, the relationship with sister genera morphologically similar to Robertgurneya was also molecularly confirmed.
Tree Species Drive the Diversity of Epiphytic Bryophytes in the Alpine Forest Ecosystem: A Case Study in Tibet
Authors: Gu Jiqi , Song Xiaotong , Liao Yujia , Ye Yanhui , Wang Ruihong , Ma Heping , Shao Xiaoming
Published: December 2022

Epiphytic bryophytes can maintain water, recycle nutrients, and provide habitats for many other organisms in the forest ecosystem. Describing the spatial change of epiphytic bryophytes plays an important role in understanding the potential factors of diversity distribution. The study aimed to determine the influence of environmental factors on their diversity and assemblage in the alpine forest ecosystem of Sygera Mountain. We considered 72 trees, taking into account 1152 quadrats to research the study. Our results showed bryophyte richness first exhibited a hump-shaped pattern and then increased along elevation, reaching a peak at 3500 m. The richness of the eastern aspect was higher than that of the west. Diversity of Quercus semecarpifolia was the highest. There was significant variation among elevations of bryophytes assemblages, especially for the biotopes at 3900 m and 4100 m. The differences among bryophytes assemblages on aspects and tree species were less apparent. Diameter at breast height and elevation influenced assemblage composition. Elevation, tree species, and tree properties drive the diversity of epiphytic bryophytes, and tree species were significantly related to the variation in the diversity of bryophytes. These results are helpful for understanding the relationship between the spatial distribution pattern of bryophytes and the environment.
Human Genetic Research in Wallacea and Sahul: Recent Findings and Future Prospects
Authors: Taufik Leonard , Teixeira João C. , Llamas Bastien , Sudoyo Herawati , Tobler Raymond , Purnomo Gludhug A.
Published: December 2022

Genomic sequence data from worldwide human populations have provided a range of novel insights into our shared ancestry and the historical migrations that have shaped our global genetic diversity. However, a comprehensive understanding of these fundamental questions has been impeded by the lack of inclusion of many Indigenous populations in genomic surveys, including those from the Wallacean archipelago (which comprises islands of present-day Indonesia located east and west of Wallace’s and Lydekker’s Lines, respectively) and the former continent of Sahul (which once combined New Guinea and Australia during lower sea levels in the Pleistocene). Notably, these regions have been important areas of human evolution throughout the Late Pleistocene, as documented by diverse fossil and archaeological records which attest to the regional presence of multiple hominin species prior to the arrival of anatomically modern human (AMH) migrants. In this review, we collate and discuss key findings from the past decade of population genetic and phylogeographic literature focussed on the hominin history in Wallacea and Sahul. Specifically, we examine the evidence for the timing and direction of the ancient AMH migratory movements and subsequent hominin mixing events, emphasising several novel but consistent results that have important implications for addressing these questions. Finally, we suggest potentially lucrative directions for future genetic research in this key region of human evolution.
Chemotherapy Drugs Act Differently in the Expression and Somatic Mobilization of the mariner Transposable Element in Drosophila simulans
Authors: Bernardt Taís Maus , Treviso Estéfani Maria , Cancian Mariana , Silva Monica de Medeiros , da Rocha João Batista Teixeira , Loreto Elgion Lucio Silva
Published: December 2022

Transposable elements (TEs) are abundant in genomes. Their mobilization can lead to genetic variability that is useful for evolution, but can also have deleterious biological effects. Somatic mobilization (SM) has been linked to degenerative diseases, such as Alzheimer’s disease and cancer. We used a Drosophila simulans strain, in which SM can be measured by counting red spots in the eyes, to investigate how chemotherapeutic agents affect expression and SM of the mariner TE. Flies were treated with Cisplatin, Dacarbazine, and Daunorubicin. After acute exposure, relative expression of mariner was quantified by RT-qPCR and oxidative stress was measured by biochemical assays. Exposure to 50 and 100 µg/mL Cisplatin increased mariner expression and ROS levels; catalase activity increased at 100 µg/mL. With chronic exposure, the number of spots also increased, indicating higher mariner SM. Dacarbazine (50 and 100 µg/mL) did not significantly alter mariner expression or mobilization or ROS levels, but decreased catalase activity (100 µg/mL). Daunorubicin (25 and 50 µM) increased mariner expression, but decreased mariner SM. ROS and catalase activity were also reduced. Our data suggest that stress factors may differentially affect the expression and SM of TEs. The increase in mariner transposase gene expression is necessary, but not sufficient for mariner SM.
A Rapid Systematic Review on the Experiences of Cancer Survivors Residing in Rural Areas during the COVID-19 Pandemic
Authors: Nelson David , Cooke Samuel , McLeod Ben , Nanyonjo Agnes , Kane Ros , Gussy Mark
Published: December 2022

The COVID-19 pandemic has caused considerable disruption to cancer care and may have exacerbated existing challenges already faced by cancer survivors from rural areas. This has created a need for a rapid evidence synthesis to inform the development of tailored interventions that address the specific needs of rural cancer survivors who continue to be affected by the pandemic. The review was conducted following guidance from the Cochrane Rapid Review Methods Group. Database searches were performed via the EBSCOHost interface (includes MEDLINE, CINAHL, PsycINFO) on 25 May 2022 and supplemented with searches on Google Scholar. Peer-reviewed articles published after March 2020 that reported primary data on the experiences of cancer survivors residing in rural and remote settings during the pandemic were included. Findings were tabulated and written up narratively. Fourteen studies were included. The COVID-19 pandemic had a mostly detrimental impact on the experiences of rural cancer survivors. People’s individual coping mechanisms were challenging for a range of reasons. Specifically, the pandemic impacted on their ability to access testing, treatment, check-ups and supportive care, their ability to maintain and access social support with close friends and family, as well as negative consequences to their finances and emotional wellbeing with some reporting feelings of psychological distress including depression and anxiety. This review provides important insight into the experiences of rural cancer survivors that may help inform tailored support in line with the needs and challenges faced because of the pandemic.
Shame-Based Experiences of Homophobic Bullying and Mental Health: The Mediating Role of Self-Compassionate Actions
Authors: Seabra Daniel , Gato Jorge , Carreiras Diogo , Petrocchi Nicola , Salvador Maria do Céu
Published: December 2022

Homophobic experiences with traumatic characteristics related to shame are more frequent among sexual minority (SM) than heterosexual individuals. Concurrently, SM individuals present higher levels of psychopathology and transdiagnostic processes (e.g., shame) than heterosexual individuals. Self-compassion has been identified as a protective mechanism that counteracts the effects of shame. The current study aimed to analyse which components of self-compassion affect mental health and test the mediating role of self-compassion in the relationship between feelings of shame in traumatic homophobic bullying experiences (THBEs) and psychopathology indicators (depression, anxiety, and social anxiety symptoms). In this study, 190 Portuguese SM individuals (Mage = 28.3, SD = 7.5) completed self-report measures assessing traumatic experiences, self-compassion, and psychopathology. Data were explored with SPSS and AMOS. Regression analyses showed that internal shame felt during THBE and compassionate actions predicted psychopathology outcomes. Mediation analyses revealed that internal shame during a THBE had a significant indirect effect on all psychopathology outcomes through compassionate actions. In other words, internal shame during a THBE was significantly associated with depression, anxiety, and social anxiety, and these relationships were partially mediated by compassionate actions. Our results reinforce the importance of developing compassionate actions towards the self as a possible protective factor for psychopathology among SM individuals.
Traditional Beliefs, Practices, and Migration: A Risk to Malaria Transmission in Rural Nepal
Authors: Awasthi Kiran Raj , Jancey Jonine , Clements Archie C. A. , Sah Rohit Kumar , Koirala Madan Prasad , Chalise Binaya , Leavy Justine E.
Published: December 2022

The study aimed to explore sociocultural factors influencing the risk of malaria and practices and beliefs towards malaria prevention, transmission and treatment in a remote village in Khatyad Rural Municipality (KRM) of Nepal. A sequential exploratory mixed methods approach was used. Qualitative data were collected through 25 one-on-one, in-depth interviews followed by a face-to-face household survey (n = 218) among people from a village in KRM believed to have a high risk of malaria. Traditional practices such as Chhaupadi requiring the seclusion of women during menstruation and post-partum, transhumance, and reliance on traditional healers for the management of malaria were common practices in the village. The household survey found 98.1% of women faced menstrual exile either inside the house or in a separate hut, with 64.2% not having access to Long-lasting Insecticidal Nets (LLINs). Hardships and economic constraints compelled villagers to migrate seasonally for work to malaria-endemic areas in India, thereby exposing themselves to the risk of malaria. Persistent traditional beliefs and seasonal migration could threaten the elimination goals set by the national malaria program.
Associations between 24-h Movement Behavior and Internet Addiction in Adolescents: A Cross-Sectional Study
Authors: Ma Caizhen , Yan Jin , Hu Hejue , Shi Chongyan , Li Feng , Zeng Xinyue
Published: December 2022

Objective: This study aimed to explore the relationship between 24-h activity behavior and Chinese adolescents’ Internet addiction. Methods: A survey of 2045 adolescents (56.5% boys) was conducted, and adolescents’ 24-h movement behavior and Internet addiction were measured via a questionnaire. Descriptive statistics were used to analyze the basic situation of the respondents; chi-square analysis was used to compare gender differences, and logistic regression was used to analyze the relationship between 24-h exercise guide entries and Internet addiction. Results: From the number of 24-h movement behavior guides, 25.3% of the children did not meet the recommended amount of any kind of activity behavior guide, while 50.4% and 21.7% of the children reached the recommended amount of one and two activity behavior guidelines, respectively; only 3.2% of the children met the recommended amount of all three activity behavior guidelines. Adolescents who did not meet the recommended 24-h activity behavior guidelines were more likely to have Internet addiction (OR = 8.46, 95 CI = 3.06–23.36), and were more likely to have one item (OR = 4.50, 95 CI = 1.64–12.39) or two items (OR = 3.12, 95 CI = 1.11–8.74). Conclusions: Physical activity, static behavior, and sleep may all have a greater impact on adolescents’ Internet addiction, among which physical activity has a greater impact on adolescents’ Internet addiction. Different combinations of 24-h movement behavior have different effects on adolescents’ Internet addiction.
Life Satisfaction and Academic Engagement in Chileans Undergraduate Students of the University of Atacama
Authors: Burgos-Videla Carmen , Jorquera-Gutiérrez Ricardo , López-Meneses Eloy , Bernal Cesar
Published: December 2022

The growing problem of mental health in the university population, as a consequence of the COVID-19 pandemic, has generated the need to consider positive variables to address this situation. Life satisfaction and academic engagement are two constructs that emerge as conceptual tools oriented in this direction. The present study sought to describe the effect of academic engagement on life satisfaction in a sample of Chilean university students. A cross-sectional co-relational design was used. A total of 370 university students participated, 72.4% female and 27.6% male, aged beitive effect of engagement on life satisfaction was demonstrated, where the dimensions vigor (β = 0.462; p < 0.01) and dedication (β = 0.465; p < 0.01) acted as significant predictors (χ2 = 87.077, gl = 32, p < 0.01; χ2/gl = 2.721; CFI = 975; TLI = 0.964; RMSEA = 0.068). The proposed model showed factorial invariance according to sex. The usefulness of employing these constructs as a way to manage the well-being and mental health of students in university institutions is discussed.
Longitudinal Study Comparing Mental Health Outcomes in Frontline Emergency Department Healthcare Workers through the Different Waves of the COVID-19 Pandemic
Authors: Th’ng Francesca , Rao Kailing Adriel , Ge Lixia , Neo Hwee Nah , Molina Joseph Antonio De , Lim Wei Yang , Mao Desmond
Published: December 2022

As countries transition from the COVID-19 pandemic to endemic status, healthcare systems continue to be under pressure. We aimed to quantify changes in depression, anxiety, stress and post-traumatic stress disorder (PTSD) between 3 cohorts (2020, 2021 and 2022) of our Emergency Department (ED) healthcare workers (HCWs) and those who had worked through all 3 phases of the pandemic; and identify factors associated with poorer mental health outcomes (MHOs). In this longitudinal single-centre study in Singapore, three surveys were carried out yearly (2020, 2021 and 2022) since the COVID-19 outbreak. Depression, anxiety and stress were measured using DASS-21, and PTSD was measured using IES-R. A total of 327 HCWs (90.1%) participated in 2020, 279 (71.5%) in 2021 and 397 (92.8%) in 2022. In 2022, ED HCWs had greater concerns about workload (Mean score ± SD: 2022: 4.81 ± 0.86, vs. 2021: 4.37 ± 0.89, vs. 2020: 4.04 ± 0.97) and perceived to have less workplace support (2022: 4.48 ± 0.76, vs. 2021: 4.66 ± 0.70, vs. 2020: 4.80 ± 0.69). There was overall worsening depression (27.5% in 2020, 29.7% in 2021 and 32.2% in 2022) and stress (12.2% in 2020, 14.0% in 2021 and 17.4% in 2022). Healthcare assistants as a subgroup had improving MHOs. ED HCWs who were female and had psychiatric history, were living with the elderly, and had concerns about their working environment, workload and infection had poorer MHOs. This study will guide us in refining existing and devising more focused interventions to further support our ED HCWs’ wellbeing.
Forecasting HFMD Cases Using Weather Variables and Google Search Queries in Sabah, Malaysia
Authors: Jayaraj Vivek Jason , Hoe Victor Chee Wai
Published: December 2022

HFMD is a viral-mediated infectious illness of increasing public health importance. This study aimed to develop a forecasting tool utilizing climatic predictors and internet search queries for informing preventive strategies in Sabah, Malaysia. HFMD case data from the Sabah State Health Department, climatic predictors from the Malaysia Meteorological Department, and Google search trends from the Google trends platform between the years 2010–2018 were utilized. Cross-correlations were estimated in building a seasonal auto-regressive moving average (SARIMA) model with external regressors, directed by measuring the model fit. The selected variables were then validated using test data utilizing validation metrics such as the mean average percentage error (MAPE). Google search trends evinced moderate positive correlations to the HFMD cases (r0–6weeks: 0.47–0.56), with temperature revealing weaker positive correlations (r0–3weeks: 0.17–0.22), with the association being most intense at 0–1 weeks. The SARIMA model, with regressors of mean temperature at lag 0 and Google search trends at lag 1, was the best-performing model. It provided the most stable predictions across the four-week period and produced the most accurate predictions two weeks in advance (RMSE = 18.77, MAPE = 0.242). Trajectorial forecasting oscillations of the model are stable up to four weeks in advance, with accuracy being the highest two weeks prior, suggesting its possible usefulness in outbreak preparedness.
Knowledge, Attitudes and Perception of Mosquito Control in Different Citizenship Regimes within and Surrounding the Malakasa Open Accommodation Refugee Camp in Athens, Greece
Authors: Kolimenakis Antonios , Tsesmelis Demetrios , Richardson Clive , Balatsos Georgios , Milonas Panagiotis G. , Stefopoulou Angeliki , Horstick Olaf , Yakob Laith , Papachristos Dimitrios P. , Michaelakis Antonios
Published: December 2022

The study aims to evaluate the Knowledge, Attitude and Perception (KAP) of different societal groups concerning the implementation of targeted community-based mosquito surveillance and control interventions in different citizenship regimes. Targeted surveys were carried out within Malakasa camp for migrants and refugees, neighboring residential areas and urban areas in the wider Athens metropolitan area to investigate different knowledge levels and the role that both local and migrant communities can play in the implementation of community-based interventions based on their attitudes and perceptions. A scoring system was used to rate the collected responses. Results indicate different levels of KAP among the various groups of respondents and different priorities that should be considered in the design and execution of community interventions. Findings indicate a lower level of Knowledge Attitudes and Perceptions for the migrants, while the rate of correct answers for Perception significantly improved for migrants following a small-scale information session. The study highlights disparities in the levels of knowledge for certain public health issues and the feasibility of certain approaches for alleviating health-related challenges such as mosquito-borne diseases. Findings suggest that essential preparedness is needed by public authorities to respond to public health challenges related to migration and the spread of vector-borne diseases.
The Effect of Mother Phubbing on Young Children’s Emotional and Behavioral Problems: A Moderated Mediation Model of Mother–Child Attachment and Parenting Stress
Authors: Lv Huan , Ye Wenyu , Chen Suiqing , Zhang Hongfeng , Wang Ruiming
Published: December 2022

Phubbing—the act of ignoring someone physically present in favor of a mobile phone—is increasingly prevalent in families, and mothers’ phubbing behaviors may have a particularly important effect on young children’s development. Accordingly, this study explores the mediating role of mother–child attachment in the relationship between mother phubbing and children’s emotional and behavioral problems, as well as the role of maternal parenting stress in moderating the mediation effect. A total of 988 mothers of young children (mean age = 4.93, SD = 0.94) were surveyed using four scales, and the resulting data was statistically analyzed. The study found that (1) mother phubbing was significantly and positively correlated with children’s emotional and behavioral problems (r = 0.19, p < 0.01), (2) mother–child attachment mediated the relationship between mother phubbing and children’s emotional and behavioral problems, and (3) the relationship between mother–child attachment and children’s emotional and behavioral problems was moderated by maternal parenting stress. The present study offers fresh evidence of how mother phubbing affects young children’s emotional and behavioral difficulties. The need to reduce maternal parental stress and buffer mothers from its effects are highlighted as vital factors in promoting secure mother–child attachment and alleviating young children’s problems.
Identification of Important Factors Causing Developmental Arrest in Cloned Pig Embryos by Embryo Biopsy Combined with Microproteomics
Authors: Zhang Yuxing , Yang Liusong , Zhang Yiqian , Liang Yalin , Zhao Huaxing , Li Yanan , Cai Gengyuan , Wu Zhenfang , Li Zicong
Published: December 2022

The technique of pig cloning holds great promise for the livestock industry, life science, and biomedicine. However, the prenatal death rate of cloned pig embryos is extremely high, resulting in a very low cloning efficiency. This limits the development and application of pig cloning. In this study, we utilized embryo biopsy combined with microproteomics to identify potential factors causing the developmental arrest in cloned pig embryos. We verified the roles of two potential regulators, PDCD6 and PLK1, in cloned pig embryo development. We found that siRNA-mediated knockdown of PDCD6 reduced mRNA and protein expression levels of the pro-apoptotic gene, CASP3, in cloned pig embryos. PDCD6 knockdown also increased the cleavage rate and blastocyst rate of cloned porcine embryos. Overexpression of PLK1 via mRNA microinjection also improved the cleavage rate of cloned pig embryos. This study provided a new strategy to identify key factors responsible for the developmental defects in cloned pig embryos. It also helped establish new methods to improve pig cloning efficiency, specifically by correcting the expression pattern of PDCD6 and PLK1 in cloned pig embryos.
Nanoparticles for Topical Application in the Treatment of Skin Dysfunctions—An Overview of Dermo-Cosmetic and Dermatological Products
Authors: Raszewska-Famielec Magdalena , Flieger Jolanta
Published: December 2022

Nanomaterials (NM) arouse interest in various fields of science and industry due to their composition-tunable properties and the ease of modification. They appear currently as components of many consumer products such as sunscreen, dressings, sports clothes, surface-cleaning agents, computer devices, paints, as well as pharmaceutical and cosmetics formulations. The use of NPs in products for topical applications improves the permeation/penetration of the bioactive compounds into deeper layers of the skin, providing a depot effect with sustained drug release and specific cellular and subcellular targeting. Nanocarriers provide advances in dermatology and systemic treatments. Examples are a non-invasive method of vaccination, advanced diagnostic techniques, and transdermal drug delivery. The mechanism of action of NPs, efficiency of skin penetration, and potential threat to human health are still open and not fully explained. This review gives a brief outline of the latest nanotechnology achievements in products used in topical applications to prevent and treat skin diseases. We highlighted aspects such as the penetration of NPs through the skin (influence of physical–chemical properties of NPs, the experimental models for skin penetration, methods applied to improve the penetration of NPs through the skin, and methods applied to investigate the skin penetration by NPs). The review summarizes various therapies using NPs to diagnose and treat skin diseases (melanoma, acne, alopecia, vitiligo, psoriasis) and anti-aging and UV-protectant nano-cosmetics.
Microfibril Associated Protein 5 (MFAP5) Is Related to Survival of Ovarian Cancer Patients but Not Useful as a Prognostic Biomarker
Authors: Kujawa Katarzyna Aleksandra , Zembala-Nożynska Ewa , Syrkis Joanna Patrycja , Cortez Alexander Jorge , Kupryjańczyk Jolanta , Lisowska Katarzyna Marta
Published: December 2022

Ovarian cancer (OC) is usually diagnosed late due to its nonspecific symptoms and lack of reliable tools for early diagnostics and screening. OC studies concentrate on the search for new biomarkers and therapeutic targets. This study aimed to validate the MFAP5 gene, and its encoded protein, as a potential prognostic biomarker. In our previous study, we found that patients with high-grade serous OC who had higher MFAP5 mRNA levels had shorter survival, as compared with those with lower levels. Here, we used the Kaplan-Meier Plotter and CSIOVDB online tools to analyze possible associations of MFAP5 expression with survival and other clinico-pathological features. In these analyses, higher MFAP5 mRNA expression was observed in the more advanced FIGO stages and high-grade tumors, and was significantly associated with shorter overall and progression-free survival. Next, we analyzed the expression of the MFAP5 protein by immunohistochemistry (IHC) in 108 OC samples and tissue arrays. Stronger MFAP5 expression was associated with stronger desmoplastic reaction and serous vs. non-serous histology. We found no significant correlation between IHC results and survival, although there was a trend toward shorter survival in patients with the highest IHC scores. We searched for co-expressed genes/proteins using cBioPortal and analyzed potential MFAP5 interaction networks with the STRING tool. MFAP5 was shown to interact with many extracellular matrix proteins, and was connected to the Notch signaling pathway. Therefore, although not suitable as a prognostic biomarker for evaluation with a simple diagnostic tool like IHC, MFAP5 is worth further studies as a possible therapeutic target.
High-Resolution Small RNAs Landscape Provides Insights into Alkane Adaptation in the Marine Alkane-Degrader Alcanivorax dieselolei B-5
Authors: Wei Guangshan , Li Sujie , Ye Sida , Wang Zining , Zarringhalam Kourosh , He Jianguo , Wang Wanpeng , Shao Zongze
Published: December 2022

Alkanes are widespread in the ocean, and Alcanivorax is one of the most ubiquitous alkane-degrading bacteria in the marine ecosystem. Small RNAs (sRNAs) are usually at the heart of regulatory pathways, but sRNA-mediated alkane metabolic adaptability still remains largely unknown due to the difficulties of identification. Here, differential RNA sequencing (dRNA-seq) modified with a size selection (~50-nt to 500-nt) strategy was used to generate high-resolution sRNAs profiling in the model species Alcanivorax dieselolei B-5 under alkane (n-hexadecane) and non-alkane (acetate) conditions. As a result, we identified 549 sRNA candidates at single-nucleotide resolution of 5′-ends, 63.4% of which are with transcription start sites (TSSs), and 36.6% of which are with processing sites (PSSs) at the 5′-ends. These sRNAs originate from almost any location in the genome, regardless of intragenic (65.8%), antisense (20.6%) and intergenic (6.2%) regions, and RNase E may function in the maturation of sRNAs. Most sRNAs locally distribute across the 15 reference genomes of Alcanivorax, and only 7.5% of sRNAs are broadly conserved in this genus. Expression responses to the alkane of several core conserved sRNAs, including 6S RNA, M1 RNA and tmRNA, indicate that they may participate in alkane metabolisms and result in more actively global transcription, RNA processing and stresses mitigation. Two novel CsrA-related sRNAs are identified, which may be involved in the translational activation of alkane metabolism-related genes by sequestering the global repressor CsrA. The relationships of sRNAs with the characterized genes of alkane sensing (ompS), chemotaxis (mcp, cheR, cheW2), transporting (ompT1, ompT2, ompT3) and hydroxylation (alkB1, alkB2, almA) were created based on the genome-wide predicted sRNA–mRNA interactions. Overall, the sRNA landscape lays the ground for uncovering cryptic regulations in critical marine bacterium, among which both the core and species-specific sRNAs are implicated in the alkane adaptive metabolisms.
Investigation on Roles of Packing Density and Water Film Thickness in Synergistic Effects of Slag and Silica Fume
Authors: Zhao Yunchuan , Dong Xuming , Zhou Zicun , Long Jiangfeng , Lu Guoyun , Lei Honggang
Published: December 2022

The ternary blended cement with finer slag and silica fume (SF) could improve the packing density (PD) through the filling effect. The excess water (water more than needed for filling into voids between the cement particles) can be released to improve the fresh properties and densify the microstructure which is beneficial for improving the hardened properties. To verify the hypothesis and reveal how and why (cement + slag + SF) the ternary blends could bring such advantages, the binder pastes incorporating slag and SF with various water-to-binder ratios were produced to determine the PD experimentally. To evaluate the optimum water demand (OWD) for maximum wet density, the influence of the dispersion state of the binder on PD was investigated using the wet packing density approach. The effect of PD of various binary and ternary binder systems on water film thickness (WFT), fluidity, setting time, and compressive strength development of cement paste was also investigated. The results show that the ternary blends could improve the PD and decrease the water film thickness (WFT). The enhanced PD and altered WFT are able to increase fluidity and compressive strength. The ternary blends could improve the compressive strengths by increasing PD and exerting nucleation and pozzolanic effects.
Influence of Laser Parameters on the Texturing of 420 Stainless Steel
Authors: Cunha Ângela , Bartolomeu Flávio , Silva Filipe , Trindade Bruno , Carvalho Óscar
Published: December 2022

AISI 420 martensitic stainless steel is widely used in the mould industry due to its high tensile strength, hardness, and corrosion properties. Another requirement concerning any material used for this type of application is high thermal conductivity to minimise the time between consecutive injection cycles. The surfaces of some parts of the mould may be textured and reinforced with a material with higher thermal conductivity to achieve this aim. The results of a detailed study on the texturing of annealed 420 stainless steel using a Nd:YVO4 fibre laser are presented in this work. The influence of the laser’s processing parameters (laser power, scanning speed, number of passes, and line spacing) on the dimensions of the track, microstructure, and hardness of the modified surfaces was studied. Based on the continuity and dimensions of the machined grooves, several promising textures could be produced with laser power values from 5 to 30 W, scanning speeds of 500 to 2000 mm/s, 8 passes or more, and line spacings of 40 and 50 µm. High laser powers were responsible for the dissolution of chromium carbides in the laser tracks, the incorporation of chromium in austenite, and the consequent hardening of the microstructure.
Microstructure and Corrosion Resistance of AZ91 Magnesium Alloy after Surface Remelting Treatment
Authors: Iwaszko Józef , Strzelecka Monika
Published: December 2022

The effect of surface remelting treatment on the microstructure and corrosion resistance of the AZ91 magnesium alloy was studied. The surface layer was remelted by GTAW (gas tungsten arc welding). An original two-burner system with welding torches operating in a tandem configuration was used, allowing the combination of cleaning the surface from oxides with the remelting process. The studies of the corrosion resistance of the alloy included electrochemical tests and measurements of the rate of hydrogen evolution. The results showed that surface remelting treatment leads to favorable microstructural changes, manifested in strong grain refinement and a more uniform arrangement of the β-Mg17Al12 phase. The changes in the microstructure caused by remelting and the accompanying fast crystallization contributed to an increase in the corrosion resistance of the remelted samples in comparison to their non-remelted equivalents. The results obtained on the basis of the polarization curves showed three-fold lower values of the corrosion current density in the case of the remelted material than the value of the corrosion current density determined for the starting material. In turn, in the case of measurements of the electrochemical noise and corrosion rate determined by the method of measuring the rate of hydrogen evolution, this value for the remelted alloy was two times lower. The research also showed that GTAW technology is highly effective and can be a valuable alternative to laser techniques. The complete experimental details, obtained results and their analyses are presented in this paper.
Development of Biocompatible Bulk MgZnCa Metallic Glass with Very High Corrosion Resistance in Simulated Body Fluid
Authors: Bin Shi Jie Bryan , Fong Kai Soon , Chua Beng Wah , Gupta Manoj
Published: December 2022

Magnesium-zinc-calcium (Mg-Zn-Ca) alloys as a biomaterial have attracted much attention recently, owing to their excellent biocompatibility, similar mechanical properties to natural bone, and biodegradable properties. Despite the numerous advantages of MgZnCa alloys, the rapid degradation of magnesium proved challenging as the implant in unable to retain its structural integrity for a sufficient duration of time. For metallic glasses, the capability to produce a bulk sample that is sufficiently large for useful applications have been far less successful owing to challenging processing parameters that are required for rapid cooling. In this study, Mg65Zn30Ca5 melt-spun ribbons were produced using melt-spinning followed by spark plasma sintering under high pressure (60 MPa) at different temperatures (130–170 °C) to provide an insight into the consolidation, mechanical, and corrosion behavior. Microstructural interfaces were characterized using scanning electron microscopy while the thermal stability of the amorphous phase was characterized using differential scanning calorimetry and X-ray diffraction. Here, pellets with 10 mm diameter and 10 mm height with a complete amorphous structure were achieved at a sintering temperature of 150 °C with densification as high at ~98%. Sintering at higher temperatures, while achieving higher densification, resulted in the presence of nano-crystallites. The mechanical properties were characterized using microhardness and compression tests. The hardness values of the sintered products were relatively higher to those containing crystallite phases while the ultimate compressive strength increased with increasing sintering temperature. Bio-corrosion properties were characterized via electrochemical testing with PBS as the electrolyte at 37 °C. The corrosion results suggest that the sintered samples have a significantly improved corrosion resistance as compared to as-cast samples. More notably, SPS150 (samples sintered at 150 °C) exhibited the best corrosion resistance (35× compared to as-cast in the context of corrosion current density), owing to its single-phase amorphous nature. This study clearly shows the potential of spark plasma sintering in consolidating amorphous ribbons to near-full density bulk pellets with high corrosion resistance for bio-applications.
Phosphorus-Containing Telomers as UV-Curable Binders of Solvent-Free Varnish Coatings
Authors: Kraśkiewicz Agata , Kowalczyk Agnieszka
Published: December 2022

The synthesis of novel phosphorus-containing telomers (P-telomers) was conducted via a solution-free UV-initiated telomerization process of butyl acrylate, methyl methacrylate, 2-hydroxyethyl acrylate, and styrene, different phosphorus telogens (dimethyl phosphite (DMPh), dibutyl phosphite (DBPh), diphenyl phosphite (DPPh) or dibutyl phosphate (DBP)), and a radical photoinitiator-acylphosphine oxide (APO). The course of the UV-phototelomerization process was monitored by photo-DSC and the chemical structures of telomers were assessed by FTIR. Final UV-photocurable varnish compositions consisted of prepared P-telomer syrups, crosslinking monomer (pentaerythritol triacrylate; PETIA), and a radical UV-photoinitiator (α-hydroxyalkylphenone, HAP). The influence of P- telomers on the optical and mechanical features of coatings was investigated. Relatively the highest hardness and satisfactory scratch values, as well as water and solvent resistance, were observed for varnish based on DMPh-telomers. While the strongest adhesive bond to a glass substrate was reported for DPPh-telomers. It is worth pointing out that the P-telomers did not affect the gloss values of varnishes in comparison to the telomer-free reference sample.
Adhesive Bonding of Scots Pine Wood from the Szczecinek Forest District for the Production of Garden Equipment: An Experimental Investigation
Authors: Krystofiak Tomasz , Lis Barbara , Beker Cezary
Published: December 2022

This work aimed to determine the gluability of pressure-impregnated pine wood with two protection agents used in production technologies for garden equipment and their effect on the strength, water resistance and thermal resistance of joints, as well as their susceptibility to aging. The tests were carried out on Scots pine wood (Pinus sylvestris L.) purchased from the forest districts of the Regional Directorate of State Forests in Szczecinek town, Poland. For the impregnation, two commercial protection agents were used. The pressure impregnation of the elements and gluing with the PUR adhesive were carried out by a garden furniture manufacturer. The strength and durability after aging tests of glued joints were performed in accordance with the procedure described in Technical Approval No. AT-15-2948/00 and the PN-EN ISO 9142 standard. The collected experimental data can be used in technological solutions, mainly for processes which involve gluing wood after impregnation.
Applications of Advanced Nanomaterials in Sensor Devices
Authors: Zhang Huacheng
Published: December 2022

Nanomaterials can be classified into diverse categories according to their various physical and chemical properties, dimensionality, production procedures, compositions, and homogeneity [...]
Worst-Case X-ray Photon Energies in Ultrashort Pulse Laser Processing
Authors: Böttcher Katrin , Schmitt Rahner Mayka , Stolzenberg Ulf , Kraft Sebastian , Bonse Jörn , Feist Carsten , Albrecht Daniel , Pullner Björn , Krüger Jörg
Published: December 2022

Ultrashort pulse laser processing can result in the secondary generation of unwanted X-rays if a critical laser irradiance of about 1013 W cm−2 is exceeded. Spectral X-ray emissions were investigated during the processing of tungsten and steel using three complementary spectrometers (based on CdTe and silicon drift detectors) simultaneously for the identification of a worst-case spectral scenario. Therefore, maximum X-ray photon energies were determined, and corresponding dose equivalent rates were calculated. An ultrashort pulse laser workstation with a pulse duration of 274 fs, a center wavelength of 1030 nm, pulse repetition rates between 50 kHz and 200 kHz, and a Gaussian laser beam focused to a spot diameter of 33 μm was employed in a single pulse and burst laser operation mode. Different combinations of laser pulse energy and repetition rate were utilized, keeping the average laser power constant close to the maximum power of 20 W. Peak irradiances I0 ranging from 7.3 × 1013 W cm−2 up to 3.0 × 1014 W cm−2 were used. The X-ray dose equivalent rate increases for lower repetition rates and higher pulse energy if a constant average power is used. Laser processing with burst mode significantly increases the dose rates and the X-ray photon energies. A maximum X-ray photon energy of about 40 keV was observed for burst mode processing of tungsten with a repetition rate of 50 kHz and a peak irradiance of 3 × 1014 W cm−2.
Tailoring the Topological Charge of a Superposition of Identical Parallel Laguerre–Gaussian Beams
Authors: Kotlyar Victor V. , Kovalev Alexey A. , Kozlova Elena S. , Savelyeva Alexandra A.
Published: December 2022

In optical computing machines, data can be transmitted by optical vortices, and the information can be encoded by their topological charges. Thus, some optical mechanisms are needed for performing simple arithmetic operations with the topological charges. Here, a superposition of several parallel identical Laguerre–Gaussian beams with single rings is studied. It is analytically and numerically shown that if the weighting coefficients of the superposition are real, then the total topological charge of the superposition is equal to the topological charge of each component in the initial plane and in the far field. We prove that the total topological charge of the superposition can be changed by the phase delay between the beams. In the numerical simulation, we demonstrate the incrementing and decrementing the topological charge. Potential application areas are in optical computing machines and optical data transmission.