bluuebunny/crossref_metadata_2025 huggingface dataset:
Rolling Bearing Fault Diagnosis Based on Refined Composite Multi-Scale Approximate Entropy and Optimized Probabilistic Neural Network
Authors: Ma Jianpeng , Li Zhenghui , Li Chengwei , Zhan Liwei , Zhang Guang-Zhu
Published: February 2021

A rolling bearing early fault diagnosis method is proposed in this paper, which is derived from a refined composite multi-scale approximate entropy (RCMAE) and improved coyote optimization algorithm based probabilistic neural network (ICOA-PNN) algorithm. Rolling bearing early fault diagnosis is a time-sensitive task, which is significant to ensure the reliability and safety of mechanical fault system. At the same time, the early fault features are masked by strong background noise, which also brings difficulties to fault diagnosis. So, we firstly utilize the composite ensemble intrinsic time-scale decomposition with adaptive noise method (CEITDAN) to decompose the signal at different scales, and then the refined composite multi-scale approximate entropy of the first signal component is calculated to analyze the complexity of describing the vibration signal. Afterwards, in order to obtain higher recognition accuracy, the improved coyote optimization algorithm based probabilistic neural network classifiers is employed for pattern recognition. Finally, the feasibility and effectiveness of this method are verified by rolling bearing early fault diagnosis experiment.
Identifying Student Teachers’ Inquiry-Related Questions in Biology Lessons
Authors: Hiltunen Matti , Kärkkäinen Sirpa , Keinonen Tuula
Published: February 2021

Prior research has shown that both teacher-led and recitation questions dominate in classrooms; teachers ask closed-ended questions more than open-ended questions. Even though classroom questioning has been studied in many previous studies there has been very limited research addressing the questioning of student teachers during inquiry-based biology lessons focusing on the inquiry stages: introduction, examination, and conclusion. In this study, a total of 21 lessons by 12 student teachers in primary and secondary schools were video- and audio-recorded. The recorded discussions were transcribed and the qualities of the questions were analyzed using content analysis, and the questions of student teachers were categorized into 10 different question categories. The findings revealed that primary school student teachers asked mainly for factual knowledge, concepts, and basic knowledge of species in all inquiry stages. Secondary school student teachers also asked mainly for concepts and basic knowledge of species. They also asked students to generate ideas and explain their answers, especially in the examination and conclusion stages. The present study indicates that student teachers’ questioning needs to be developed more towards higher-order questioning such as analyzing, synthetizing, and evaluating to scaffold students in inquiries and develop future teachers’ questioning skills in teacher education.
Provision of Data to Use in Artificial Intelligence Algorithms for Single Room Heating
Authors: Majetta Kristin , Clauß Christoph , Nytsch-Geusen Christoph
Published: February 2021

This paper describes a way to generate a great amount of data and to use it to find a relation between a room controller and a certain room. Therefore, simulation scenarios are defined and developed that contain different room, location, usage and controller models. With parameter variation and optimization of the corresponding controller parameters a data basis is created with about 5300 entries. On the basis of this data, machine learning algorithms like artificial neural networks can be used to investigate the relation between rooms and their best suited controllers.
Impact of Actual Weather Datasets for Calibrating White-Box Building Energy Models Base on Monitored Data
Authors: Gutiérrez González Vicente , Ramos Ruiz Germán , Fernández Bandera Carlos
Published: February 2021

The need to reduce energy consumption in buildings is an urgent task. Increasing the use of calibrated building energy models (BEM) could accelerate this need. The calibration process of these models is a highly under-determined problem that normally yields multiple solutions. Among the uncertainties of calibration, the weather file has a primary position. The objective of this paper is to provide a methodology for selecting the optimal weather file when an on-site weather station with local sensors is available and what is the alternative option when it is not and a mathematically evaluation has to be done with sensors from nearby stations (third-party providers). We provide a quality assessment of models based on the Coefficient of Variation of the Root Mean Square Error (CV(RMSE)) and the Square Pearson Correlation Coefficient (R2). The research was developed on a control experiment conducted by Annex 58 and a previous calibration study. This is based on the results obtained with the study case based on the data provided by their N2 house.
Molten Salts for Sensible Thermal Energy Storage: A Review and an Energy Performance Analysis
Authors: Caraballo Adrián , Galán-Casado Santos , Caballero Ángel , Serena Sara
Published: February 2021

A comprehensive review of different thermal energy storage materials for concentrated solar power has been conducted. Fifteen candidates were selected due to their nature, thermophysical properties, and economic impact. Three key energy performance indicators were defined in order to evaluate the performance of the different molten salts, using Solar Salt as a reference for low and high temperatures. The analysis provided evidence that nitrate-based materials are the best choice for the former and chloride-based materials are best for the latter instead of fluoride and carbonate-based candidates, mainly due to their low cost.
Multi-Objective Optimisation-Based Design of an Electric Vehicle Cabin Heating Control System for Improved Thermal Comfort and Driving Range
Authors: Cvok Ivan , Ratković Igor , Deur Joško
Published: February 2021

Modern electric vehicle heating, ventilation, and air-conditioning (HVAC) systems operate in more efficient heat pump mode, thus, improving the driving range under cold ambient conditions. Coupling those HVAC systems with novel heating technologies such as infrared heating panels (IRP) results in a complex system with multiple actuators, which needs to be optimally coordinated to maximise the efficiency and comfort. The paper presents a multi-objective genetic algorithm-based control input allocation method, which relies on a multi-physical HVAC model and a CFD-evaluated cabin airflow distribution model implemented in Dymola. The considered control inputs include the cabin inlet air temperature, blower and radiator fan air mass flows, secondary coolant loop pump speeds, and IRP control settings. The optimisation objective is to minimise total electric power consumption and thermal comfort described by predictive mean vote (PMV) index. Optimisation results indicate that HVAC and IRP controls are effectively decoupled, and that a significant reduction of power consumption (typically from 20% to 30%) can be achieved using IRPs while maintaining the same level of thermal comfort. The previously proposed hierarchical HVAC control strategy is parameterised and extended with a PMV-based controller acting via IRP control inputs. The performance is verified through simulations in a heat-up scenario, and the power consumption reduction potential is analysed for different cabin air temperature setpoints.
A Back-of-Queue Model of a Signal-Controlled Intersection Approach Developed Based on Analysis of Vehicle Driver Behavior
Authors: Macioszek Elżbieta , Iwanowicz Damian
Published: February 2021

In smart cities, it is expected that transport, communication as well as the movement of people and goods will take place in the shortest possible time while maintaining a high level of safety. In recent years, due to the significant increase in the number of passengers and vehicles on the road and the capacity limitations of transport networks, it has become necessary to use new technologies for intelligent control and traffic management. Intelligent transport systems use advanced technologies in the field of data gathering, information processing, and traffic control to meet current transport needs. To be able to effectively control and manage road traffic, it is necessary to have reliable mathematical models that allow for a faithful representation of the real traffic conditions. Models of this type are usually the basis of complex algorithms used in practice in road traffic control. The application of appropriate models reflecting the behavior of road users contributes to the reduction of congestion, the vehicles travel time on the transport network, fuel consumption and the emissions, which in turn support broadly understood energy savings. The article proposes a model that allows for the estimation of the maximum queue size at the signal-controlled intersection approach (so-called: maximum back-of-queue). This model takes into account the most important traffic characteristics of the vehicles forming this queue. The verification allowed for the conclusion that the proposed model is characterized by high compliance with the actual traffic and road conditions at the intersections with signal controllers located in built-up areas in Poland. The obtained compliance confirms the possibility of using the model for practical applications in calculating the maximum back-of-queue at signal-controlled intersections located in built-up areas in Poland.
A Novel Tree Biomass Estimation Model Applying the Pipe Model Theory and Adaptable to UAV-Derived Canopy Height Models
Authors: Machimura Takashi , Fujimoto Ayana , Hayashi Kiichiro , Takagi Hiroaki , Sugita Satoru
Published: February 2021

Aiming to develop a new tree biomass estimation model that is adaptable to airborne observations of forest canopies by unmanned aerial vehicles (UAVs), we applied two theories of plant form; the pipe model theory (PMT) and the statical model of plant form as an extension of the PMT for tall trees. Based on these theories, tree biomass was formulated using an individual tree canopy height model derived from a UAV. The advantage of this model is that it does not depend on diameter at breast height which is difficult to observe using remote-sensing techniques. We also proposed a treetop detection method based on the fractal geometry of the crown and stand. Comparing surveys in plantations of Japanese cedar (Cryptomeria japonica D. Don) and Japanese cypress (Chamaecyparis obtusa Endl.) in Japan, the root mean square error (RMSE) of the estimated stem volume was 0.26 m3 and was smaller than or comparative to that of models using different methodologies. The significance of this model is that it contains only one empirical parameter to be adjusted which was found to be rather stable among different species and sites, suggesting the wide adaptability of the model. Finally, we demonstrated the potential applicability of the model to light detection and ranging (LiDAR) data which can provide vertical leaf density distribution.
The Sex Determination Cascade in the Silkworm
Authors: Yang Xu , Chen Kai , Wang Yaohui , Yang Dehong , Huang Yongping
Published: February 2021

In insects, sex determination pathways involve three levels of master regulators: primary signals, which determine the sex; executors, which control sex-specific differentiation of tissues and organs; and transducers, which link the primary signals to the executors. The primary signals differ widely among insect species. In Diptera alone, several unrelated primary sex determiners have been identified. However, the doublesex (dsx) gene is highly conserved as the executor component across multiple insect orders. The transducer level shows an intermediate level of conservation. In many, but not all examined insects, a key transducer role is performed by transformer (tra), which controls sex-specific splicing of dsx. In Lepidoptera, studies of sex determination have focused on the lepidopteran model species Bombyx mori (the silkworm). In B. mori, the primary signal of sex determination cascade starts from Fem, a female-specific PIWI-interacting RNA, and its targeting gene Masc, which is apparently specific to and conserved among Lepidoptera. Tra has not been found in Lepidoptera. Instead, the B. mori PSI protein binds directly to dsx pre-mRNA and regulates its alternative splicing to produce male- and female-specific transcripts. Despite this basic understanding of the molecular mechanisms underlying sex determination, the links among the primary signals, transducers and executors remain largely unknown in Lepidoptera. In this review, we focus on the latest findings regarding the functions and working mechanisms of genes involved in feminization and masculinization in Lepidoptera and discuss directions for future research of sex determination in the silkworm.
Age Estimation Using the Cameriere Methods of Open Apices: A Meta-Analysis
Authors: Hostiuc Sorin , Diaconescu Ioana , Rusu Mugurel Constantin , Negoi Ionut
Published: February 2021

Purpose: To evaluate the actual variability of the mean difference between chronological and dental age using the Cameriere method of open apices and to test its accuracy in variable age groups. Method: We selected studies that contained data about the mean, standard deviation, and number of cases for chronological age, dental age and gender. We used a random-effects model. Statistical significance was estimated, at a p < 0.05, using prediction intervals. For the analysis of publication bias we used the funnel plot and Egger’s regression test for plot asymmetry. I2 was used to test the presence of heterogeneity between studies. The Z test was used to test for statistical differences between subgroups, with p < 0.05 being considered statistically significant. We also used 95% for confidence intervals and prediction intervals. Results: In boys, the average difference between chronological and dental age was 0.44 (0.26–0.63) years, while in girls the average difference between chronological and dental age was 0.34 (0.19–0.49) years. In the 6–7 years age group and in the 14–15 years age group, there was a statistically significant difference between dental and chronological age. Our study shows that the Cameriere method is useful for estimating the chronological age, with errors of less than one year. Conclusions: The Cameriere method of evaluating dental age using open apices is sufficiently accurate for forensic practice, at least in the 7–14 age-interval.
Psychological Distress and Well-Being among Students of Health Disciplines: The Importance of Academic Satisfaction
Authors: Franzen Jessica , Jermann Françoise , Ghisletta Paolo , Rudaz Serge , Bondolfi Guido , Tran Nguyen Toan
Published: February 2021

Background: Research on the mental health of students in health disciplines mainly focuses on psychological distress and nursing and medical students. This study aimed to investigate the psychological well-being and distress and related factors among undergraduate students training in eight different health-related tracks in Geneva, Switzerland. Methods: This cross-sectional study used established self-filled scales for anxiety, depression, stress, psychological well-being, and study satisfaction. Descriptive statistics and hierarchical regression analyses were applied. Results: In October 2019, out of 2835 invited students, 915 (32%) completed the survey. Lower academic satisfaction scores were strongly associated with depression (β = −0.26, p < 0.001), anxiety (β = −0.27, p < 0.001), and stress (β = −0.70, p < 0.001), while higher scores were associated with psychological well-being (β = 0.70, p < 0.001). Being female was strongly associated with anxiety and stress but not with depression or psychological well-being. Increased age was associated with enhanced psychological well-being. The nature of the academic training had a lesser impact on mental health and the academic year had none. Conclusion: Academic satisfaction strongly predicts depression, anxiety, stress, and psychological well-being. Training institutions should address the underlying factors that can improve students’ satisfaction with their studies while ensuring that they have access to psychosocial services that help them cope with mental distress and enhance their psychological well-being.
Mental Health Outreach via Supportive Text Messages during the COVID-19 Pandemic: Improved Mental Health and Reduced Suicidal Ideation after Six Weeks in Subscribers of Text4Hope Compared to a Control Population
Authors: Agyapong Vincent I. O. , Shalaby Reham , Hrabok Marianne , Vuong Wesley , Noble Jasmine M. , Gusnowski April , Mrklas Kelly , Li Daniel , Snaterse Mark , Surood Shireen , Cao Bo , Li Xin-Min , Greiner Russell , Greenshaw Andrew J.
Published: February 2021

Background: In March 2020, Alberta Health Services launched Text4Hope, a free mental health text-message service. The service aimed to alleviate pandemic-associated stress, generalized anxiety disorder (GAD), major depressive disorder (MDD), and suicidal propensity. The effectiveness of Text4Hope was evaluated by comparing psychiatric parameters between two subscriber groups. Methods: A comparative cross-sectional study with two arms: Text4Hope subscribers who received daily texts for six weeks, the intervention group (IG); and new Text4Hope subscribers who were yet to receive messages, the control group (CG). Logistic regression models were used in the analysis. Results: Participants in the IG had lower prevalence rates for moderate/high stress (78.8% vs. 88.0%), likely GAD (31.4% vs. 46.5%), and likely MDD (36.8% vs. 52.1%), respectively, compared to respondents in the CG. After controlling for demographic variables, the IG remained less likely to self-report symptoms of moderate/high stress (OR = 0.56; 95% CI = 0.41–0.75), likely GAD (OR = 0.55; 95% CI = 0.44–0.68), and likely MDD (OR = 0.50; 95% CI = 0.47–0.73). The mean Composite Mental Health score, the sum of mean scores on the PSS, GAD-7, and PHQ-9 was 20.9% higher in the CG. Conclusions: Text4Hope is an effective population-level intervention that helps reduce stress, anxiety, depression, and suicidal thoughts during the COVID-19 pandemic. Similar texting services should be implemented during global crises.
Assessing Differences in the Implementation of Smoke-Free Contracts—A Cross-Sectional Analysis from the School Randomized Controlled Trial X:IT
Authors: Bast Lotus Sofie , Andersen Susan , Glenstrup Stine , Damsgaard Mogens Trab , Andersen Anette
Published: February 2021

Objective: The X:IT study is a school-based smoking preventive intervention that has previously been evaluated in a large randomized controlled trial (RCT) with good effects. However, the actual effect for participating students depends on the individual implementation. The aim of this study was to examine the implementation of smoke-free contract, which is one of the three main intervention components. Specifically, we examined whether it was implemented equally across family occupational social class (OSC), separately for boys and girls, the joint effect of OSC and gender, and the participants’ own reasons for not signing a contract. Results: Overall, the smoke-free contract was well implemented; 81.8% of pupils (total N = 2.015) signed a contract (girls 85.1, boys 78.6%). We found a social gradient among girls; more than 90% were in OSC group I vs. 75% in group VI. Among boys, however, we found no difference across OSC. Boys in all the OSC groups had about half the odds (i.e., medium OSC boys: OR = 0.48 (95% CI: 0.32–0.72) of having a smoke-free contract compared to girls from a high OSC. Conclusion: future interventions should include initiatives to involve families from all OSC groups and allow for different preferences among boys and girls.
Post-Traumatic Growth among Patients after Living and Cadaveric Donor Kidney Transplantation: The Role of Resilience and Alexithymia
Authors: Tomaszek Aleksandra , Wróblewska Aleksandra , Zdankiewicz-Ścigała Elżbieta , Rzońca Patryk , Gałązkowski Robert , Gozdowska Jolanta , Lewandowska Dorota , Kosson Dariusz , Kosieradzki Maciej , Danielewicz Roman
Published: February 2021

The aim of this study was to determine the role of resilience and alexithymia in the post-traumatic growth as a response to extreme stress in patients after kidney transplantation and to determine whether there are differences in the level of posttraumatic growth in patients after living and cadaveric donor kidney transplantation. The relationships between these variables were also evaluated. The questionnaire survey of 91 kidney recipients took place in 2018 and 2019. The following tools were used: authorial post-transplant questionnaire for recipients and validated questionnaires, Post Traumatic Growth Inventory (PTGI-R), Resilience Coping Scale Questionnaire, and Toronto Alexithymia Scale Questionnaire (TAS20). The results obtained showed significant differences between the group of kidney recipients from living donors and recipients from cadaveric donors, in terms of overall post-traumatic growth, as well as changes in self-perception and a greater appreciation for life. Post-traumatic growth in both groups was related to the level of resilience and the level of alexithymia. Resilience is an accurate predictor of posttraumatic growth in general and for each of the groups of recipients separately.
Investigation of Radiation Safety Pictogram Recognition in Daily Life
Authors: Choi Kyoungho , Choi Jinhee
Published: February 2021

After the Fukushima Daiichi nuclear accident of 2011, interest regarding radiation safety in everyday life has increased considerably. This study investigates the general public’s current level of awareness of six warning pictograms in regard to medical and natural radiation safety utilized under ISO 7010, as per Korea industrial regulations. Namely, it tests whether survey respondents can recognize pictograms related to radiation safety according to their purpose, as their inability to do so poses a serious safety problem. The empirical analysis results regarding the awareness levels for radiation safety pictograms are as follows. First, 63.3% of the respondents were unable to correctly identify the pictograms; that is, their level of understandings of the six pictograms related to everyday radiation were low. Second, the mean score for the correct responses to the question of what the six pictograms indicated in relation to everyday radiation safety was also relatively low, with a mean score of 2.79 and a standard deviation of 1.447. The primary reasons for the low awareness and understanding levels were identified to be insufficient education related to radiation safety in schools. Additionally, it is necessary to revise and rectify current warning pictograms established by the Korea Industrial Standards and ISO 7010. This study is thus significant in that it identifies the level of understanding of the pictograms and suggests the need for improvement as a diversified effort toward improving everyday radiation safety.
Development of a Chatbot Program for Follow-Up Management of Workers’ General Health Examinations in Korea: A Pilot Study
Authors: Ye Byeong Jin , Kim Ju Young , Suh Chunhui , Choi Seong Pil , Choi Maro , Kim Dong Hyun , Son Byung Chul
Published: February 2021

(1) Background: Follow-up management of workers’ general health examination (WGHE) is important, but it is not currently well done. Chatbot, a type of digital healthcare tool, is used in various medical fields but has never been developed for follow-up management of WGHE in Korea. (2) Methods: The database containing results and explanations related to WGHE was constructed. Then, the channel, which connects users with the database was created. A user survey regarding effectiveness was administered to 23 healthcare providers. Additionally, interviews on applicability for occupational health services were conducted with six nurses in the agency of occupational health management. (3) Results: Chatbot was implemented on a small scale on the Amazon cloud service (AWS) EC2 using KaKaoTalk and Web Chat as user channels. Regarding the effectiveness, 21 (91.30%) rated the need for chatbots as very high; however, 11 (47.83%) rated the usability as not high. Of the 23 participants, 14 (60.87%) expressed overall satisfaction. Nurses appreciated the chatbot program as a method for resolving accessibility and as an aid for explaining examination results and follow-up management. (4) Conclusions: The effectiveness of WGHE and the applicability in the occupational health service of the chatbot program for follow-up management can be confirmed.
How Do Decision Makers and Service Providers Experience Participatory Approaches to Developing and Implementing Physical Activity Interventions with Older Adults? A Thematic Analysis
Authors: Davies Amy , French David P. , Devereux-Fitzgerald Angela , Boulton Elisabeth , Todd Chris , Phillipson Chris , McGowan Laura J. , Powell Rachael
Published: February 2021

Background: Physical activity has numerous health and well-being benefits for older adults, but many older adults are inactive. Interventions designed to increase physical activity in older adults have typically only produced small effects and have not achieved long-term changes. There is increasing interest in participatory approaches to promoting physical activity, such as co-production, co-design and place-based approaches, but they have typically involved researchers as participants. This study aimed to understand the experiences of decision-makers and service developers with the introduction of such participatory approaches when developing new physical activity programmes outside of a research setting. Methods: Semi-structured, qualitative interviews were conducted with 20 individuals who were involved in commissioning or developing the Greater Manchester Active Ageing Programme. This programme involved funding eight local authorities within Greater Manchester, England, to produce physical activity projects for older adults, involving participatory approaches. An inductive thematic analysis was conducted, structured using the Framework approach. Results: Interviewees identified important benefits of the participatory approaches. The increased involvement of older adults led to older adults contributing valuable ideas, becoming involved in and taking ownership of projects. Interviewees identified the need to move away from traditional emphases on increasing physical activity to improve health, towards focussing on social and fun elements. The accessibility of the session location and information was considered important. Challenges were also identified. In particular, it was recognised that the new approaches require significant time investment to do well, as trusting relationships with older adults and partner organisations need to be developed. Ensuring the sustainability of projects in the context of short-term funding cycles was a concern. Conclusions: Incorporating participatory approaches was perceived to yield important benefits. Interviewees highlighted that to ensure success, sufficient time needs to be provided to develop good working relationships with older adults and partner organisations. They also emphasised that sufficient funding to ensure adequate staffing and the sustainability of projects is required to allow benefits to be gained. Importantly, the implementation of these approaches appears feasible across a range of local authorities.
Alternative Friendships to Improve Men’s Health Status. The Impact of the New Alternative Masculinities’ Approach
Authors: Ríos-González Oriol , Ramis-Salas Mimar , Peña-Axt Juan Carlos , Racionero-Plaza Sandra
Published: February 2021

Background: Men who develop behaviors connected with the model of hegemonic masculinity present several health problems. Previous research has shown the types of problems that men commonly suffer in this regard such as chronic diseases, dietary disorders, and traffic accidents. To combat and overcome this situation, several campaigns, policies and recommendations have been undertaken, and consequently, their influence has been analyzed. However, there have been few investigations into the role of men’s friendship in the reduction of these physical health problems. The findings presented in this article are focused on this issue, illustrating the impact of male friendship on the shaping of healthy behaviors. Methods: Drawing upon a qualitative-based methodology articulated in a case study of the Men in Dialogue association, located in Spain, the study has followed the premises of the communicative approach, a total of 15 structured online open-ended questionnaires have been performed and analyzed. The median age of the participants is 37.5 years. Results: The findings show how men involved in Men in Dialogue are promoting a kind of masculine friendship that is improving men’s emotional well-being and, consequently, their physical health.
Scientific Mobility, Training and Entrepreneurial Skills in Health Sciences: The Spanish Case
Authors: Aceituno-Aceituno Pedro , Danvila-del-Valle Joaquín , González García Abel , Bousoño-Calzón Carlos
Published: February 2021

The activity of scientists promotes medical research in health services. However, on many occasions, these professionals do not know how to transfer their research results to the market. Therefore, it is worth providing data on aspects such as training in entrepreneurship and scientific mobility to foster knowledge transfer. This paper discusses data on the Spanish case in Health Sciences to devise effective policies in these areas. To this end, following the methodology of the Global Entrepreneurship Monitor report and the existing scientific literature, 291 researchers involved in scientific mobility in Spain have been interviewed. Of these, 90 belonged to health areas: Spanish scientists abroad (37), Scientists returned to Spain (16), and Young researchers in Spain (37). The results show that the mobile scientists in this area have more entrepreneurial and intrapreneurial intentions, have acquired more entrepreneurial skills, and have received more training in these subjects. Furthermore, there are few permanent positions for all these groups whose mobility decisions fundamentally depend on job opportunities, so the health authorities can intensify these measures to promote knowledge transfer.
Noise Annoyance in the UAE: A Twitter Case Study via a Data-Mining Approach
Authors: Peplow Andrew , Thomas Justin , AlShehhi Aamna
Published: February 2021

Noise pollution is a growing global public health concern. Among other issues, it has been linked with sleep disturbance, hearing functionality, increased blood pressure and heart disease. Individuals are increasingly using social media to express complaints and concerns about problematic noise sources. This behavior—using social media to post noise-related concerns—might help us better identify troublesome noise pollution hotspots, thereby enabling us to take corrective action. The present work is a concept case study exploring the use of social media data as a means of identifying and monitoring noise annoyance across the United Arab Emirates (UAE). We explored an extract of Twitter data for the UAE, comprising over eight million messages (tweets) sent during 2015. We employed a search algorithm to identify tweets concerned with noise annoyance and, where possible, we also extracted the exact location via Global Positioning System (GPS) coordinates) associated with specific messages/complaints. The identified noise complaints were organized in a digital database and analyzed according to three criteria: first, the main types of the noise source (music, human factors, transport infrastructures); second, exterior or interior noise source and finally, date and time of the report, with the location of the Twitter user. This study supports the idea that lexicon-based analyses of large social media datasets may prove to be a useful adjunct or as a complement to existing noise pollution identification and surveillance strategies.
EPHA2 Segregates with Microphthalmia and Congenital Cataracts in Two Unrelated Families
Authors: Harding Philippa , Toms Maria , Schiff Elena , Owen Nicholas , Bell Suzannah , Lloyd Ian Christopher , Moosajee Mariya
Published: February 2021

EPHA2 is a transmembrane tyrosine kinase receptor that, when disrupted, causes congenital and age-related cataracts. Cat-Map reports 22 pathogenic EPHA2 variants associated with congenital cataracts, variable microcornea, and lenticonus, but no previous association with microphthalmia (small, underdeveloped eye, ≥2 standard deviations below normal axial length). Microphthalmia arises from ocular maldevelopment with >90 monogenic causes, and can include a complex ocular phenotype. In this paper, we report two pathogenic EPHA2 variants in unrelated families presenting with bilateral microphthalmia and congenital cataracts. Whole genome sequencing through the 100,000 Genomes Project and cataract-related targeted gene panel testing identified autosomal dominant heterozygous mutations segregating with the disease: (i) missense c.1751C>T, p.(Pro584Leu) and (ii) splice site c.2826-9G>A. To functionally validate pathogenicity, morpholino knockdown of epha2a/epha2b in zebrafish resulted in significantly reduced eye size ± cataract formation. Misexpression of N-cadherin and retained fibre cell nuclei were observed in the developing lens of the epha2b knockdown morphant fish by 3 days post-fertilisation, which indicated a putative mechanism for microphthalmia pathogenesis through disruption of cadherin-mediated adherens junctions, preventing lens maturation and the critical signals stimulating eye growth. This study demonstrates a novel association of EPHA2 with microphthalmia, suggesting further analysis of pathogenic variants in unsolved microphthalmia cohorts may increase molecular diagnostic rates.
The Potential of Hydrogen Sulfide Donors in Treating Cardiovascular Diseases
Authors: Wang Yi-Zhen , Ngowi Ebenezeri Erasto , Wang Di , Qi Hui-Wen , Jing Mi-Rong , Zhang Yan-Xia , Cai Chun-Bo , He Qing-Lin , Khattak Saadullah , Khan Nazeer Hussain , Jiang Qi-Ying , Ji Xin-Ying , Wu Dong-Dong
Published: February 2021

Hydrogen sulfide (H2S) has long been considered as a toxic gas, but as research progressed, the idea has been updated and it has now been shown to have potent protective effects at reasonable concentrations. H2S is an endogenous gas signaling molecule in mammals and is produced by specific enzymes in different cell types. An increasing number of studies indicate that H2S plays an important role in cardiovascular homeostasis, and in most cases, H2S has been reported to be downregulated in cardiovascular diseases (CVDs). Similarly, in preclinical studies, H2S has been shown to prevent CVDs and improve heart function after heart failure. Recently, many H2S donors have been synthesized and tested in cellular and animal models. Moreover, numerous molecular mechanisms have been proposed to demonstrate the effects of these donors. In this review, we will provide an update on the role of H2S in cardiovascular activities and its involvement in pathological states, with a special focus on the roles of exogenous H2S in cardiac protection.
Structural Communication between the E. coli Chaperones DnaK and Hsp90
Authors: Grindle Matthew P. , Carter Ben , Alao John Paul , Connors Katherine , Tehver Riina , Kravats Andrea N.
Published: February 2021

The 70 kDa and 90 kDa heat shock proteins Hsp70 and Hsp90 are two abundant and highly conserved ATP-dependent molecular chaperones that participate in the maintenance of cellular homeostasis. In Escherichia coli, Hsp90 (Hsp90Ec) and Hsp70 (DnaK) directly interact and collaborate in protein remodeling. Previous work has produced a model of the direct interaction of both chaperones. The locations of the residues involved have been confirmed and the model has been validated. In this study, we investigate the allosteric communication between Hsp90Ec and DnaK and how the chaperones couple their conformational cycles. Using elastic network models (ENM), normal mode analysis (NMA), and a structural perturbation method (SPM) of asymmetric and symmetric DnaK-Hsp90Ec, we extract biologically relevant vibrations and identify residues involved in allosteric signaling. When one DnaK is bound, the dominant normal modes favor biological motions that orient a substrate protein bound to DnaK within the substrate/client binding site of Hsp90Ec and release the substrate from the DnaK substrate binding domain. The presence of one DnaK molecule stabilizes the entire Hsp90Ec protomer to which it is bound. Conversely, the symmetric model of DnaK binding results in steric clashes of DnaK molecules and suggests that the Hsp90Ec and DnaK chaperone cycles operate independently. Together, this data supports an asymmetric binding of DnaK to Hsp90Ec.
Overexpression of miR-1306-5p, miR-3195, and miR-3914 Inhibits Ameloblast Differentiation through Suppression of Genes Associated with Human Amelogenesis Imperfecta
Authors: Yoshioka Hiroki , Wang Yin-Ying , Suzuki Akiko , Shayegh Meysam , Gajera Mona V. , Zhao Zhongming , Iwata Junichi
Published: February 2021

Amelogenesis imperfecta is a congenital form of enamel hypoplasia. Although a number of genetic mutations have been reported in humans, the regulatory network of these genes remains mostly unclear. To identify signatures of biological pathways in amelogenesis imperfecta, we conducted bioinformatic analyses on genes associated with the condition in humans. Through an extensive search of the main biomedical databases, we found 56 genes in which mutations and/or association/linkage were reported in individuals with amelogenesis imperfecta. These candidate genes were further grouped by function, pathway, protein–protein interaction, and tissue-specific expression patterns using various bioinformatic tools. The bioinformatic analyses highlighted a group of genes essential for extracellular matrix formation. Furthermore, advanced bioinformatic analyses for microRNAs (miRNAs), which are short non-coding RNAs that suppress target genes at the post-transcriptional level, predicted 37 candidates that may be involved in amelogenesis imperfecta. To validate the miRNA–gene regulation association, we analyzed the target gene expression of the top seven candidate miRNAs: miR-3195, miR-382-5p, miR-1306-5p, miR-4683, miR-6716-3p, miR-3914, and miR-3935. Among them, miR-1306-5p, miR-3195, and miR-3914 were confirmed to regulate ameloblast differentiation through the regulation of genes associated with amelogenesis imperfecta in AM-1 cells, a human ameloblastoma cell line. Taken together, our study suggests a potential role for miRNAs in amelogenesis imperfecta.
Macrophage Function and the Role of GSK3
Authors: Patel Sarvatit , Werstuck Geoff H.
Published: February 2021

Macrophages are present in nearly all vertebrate tissues, where they respond to a complex variety of regulatory signals to coordinate immune functions involved in tissue development, metabolism, homeostasis, and repair. Glycogen synthase kinase 3 (GSK3) is a ubiquitously expressed protein kinase that plays important roles in multiple pathways involved in cell metabolism. Dysregulation of GSK3 has been implicated in several prevalent metabolic disorders, and recent findings have highlighted the importance of GSK3 activity in the regulation of macrophages, especially with respect to the initiation of specific pathologies. This makes GSK3 a potential therapeutic target for the development of novel drugs to modulate immunometabolic responses. Here, we summarize recent findings that have contributed to our understanding of how GSK3 regulates macrophage function, and we discuss the role of GSK3 in the development of metabolic disorders and diseases.
Exploring Molecular Contacts of MUC1 at CIN85 Binding Interface to Address Future Drug Design Efforts
Authors: Gulotta Maria Rita , Vittorio Serena , Gitto Rosaria , Perricone Ugo , De Luca Laura
Published: February 2021

The modulation of protein-protein interactions (PPIs) by small molecules represents a valuable strategy for pharmacological intervention in several human diseases. In this context, computer-aided drug discovery techniques offer useful resources to predict the network of interactions governing the recognition process between protein partners, thus furnishing relevant information for the design of novel PPI modulators. In this work, we focused our attention on the MUC1-CIN85 complex as a crucial PPI controlling cancer progression and metastasis. MUC1 is a transmembrane glycoprotein whose extracellular domain contains a variable number of tandem repeats (VNTRs) regions that are highly glycosylated in normal cells and under-glycosylated in cancer. The hypo-glycosylation fosters the exposure of the backbone to new interactions with other proteins, such as CIN85, that alter the intracellular signalling in tumour cells. Herein, different computational approaches were combined to investigate the molecular recognition pattern of MUC1-CIN85 PPI thus unveiling new structural information useful for the design of MUC1-CIN85 PPI inhibitors as potential anti-metastatic agents.
Identification of Active Site Residues of the Siderophore Synthesis Enzyme PvdF and Evidence for Interaction of PvdF with a Substrate-Providing Enzyme
Authors: Philem Priya , Kleffmann Torsten , Gai Sinan , Hawkins Bill C. , Wilbanks Sigurd M. , Lamont Iain L.
Published: February 2021

The problematic opportunistic pathogen Pseudomonas aeruginosa secretes a siderophore, pyoverdine. Pyoverdine scavenges iron needed by the bacteria for growth and for pathogenicity in a range of different infection models. PvdF, a hydroxyornithine transformylase enzyme, is essential for pyoverdine synthesis, catalysing synthesis of formylhydroxyornithine (fOHOrn) that forms part of the pyoverdine molecule and provides iron-chelating hydroxamate ligands. Using a mass spectrometry assay, we confirm that purified PvdF catalyses synthesis of fOHOrn from hydroxyornithine and formyltetrahydrofolate substrates. Site directed mutagenesis was carried out to investigate amino acid residues predicted to be required for enzymatic activity. Enzyme variants were assayed for activity in vitro and also in vivo, through measuring their ability to restore pyoverdine production to a pvdF mutant strain. Variants at two putative catalytic residues N168 and H170 greatly reduced enzymatic activity in vivo though did not abolish activity in vitro. Change of a third residue D229 abolished activity both in vivo and in vitro. A change predicted to block entry of N10-formyltetrahydrofolate (fTHF) to the active site also abolished activity both in vitro and in vivo. A co-purification assay showed that PvdF binds to an enzyme PvdA that catalyses synthesis of hydroxyornithine, with this interaction likely to increase the efficiency of fOHOrn synthesis. Our findings advance understanding of how P. aeruginosa synthesises pyoverdine, a key factor in host–pathogen interactions.
Targeting lncRNA H19/miR-29b/COL1A1 Axis Impedes Myofibroblast Activities of Precancerous Oral Submucous Fibrosis
Authors: Yu Cheng-Chia , Liao Yi-Wen , Hsieh Pei-Ling , Chang Yu-Chao
Published: February 2021

Oral submucous fibrosis (OSF) is known as a potentially malignant disorder, which may result from chemical irritation due to areca nuts (such as arecoline). Emerging evidence suggests that fibrogenesis and carcinogenesis are regulated by the interaction of long noncoding RNAs (lncRNAs) and microRNAs. Among these regulators, profibrotic lncRNA H19 has been found to be overexpressed in several fibrosis diseases. Here, we examined the expression of H19 in OSF specimens and its functional role in fibrotic buccal mucosal fibroblasts (fBMFs). Our results indicate that the aberrantly overexpressed H19 contributed to higher myofibroblast activities, such as collagen gel contractility and migration ability. We also demonstrated that H19 interacted with miR-29b, which suppressed the direct binding of miR-29b to the 3′-untranslated region of type I collagen (COL1A1). We showed that ectopic expression of miR-29b ameliorated various myofibroblast phenotypes and the expression of α-smooth muscle actin (α-SMA), COL1A1, and fibronectin (FN1) in fBMFs. In OSF tissues, we found that the expression of miR-29b was downregulated and there was a negative correlation between miR-29b and these fibrosis markers. Lastly, we demonstrate that arecoline stimulated the upregulation of H19 through the transforming growth factor (TGF)-β pathway. Altogether, this study suggests that increased TGF-β secretion following areca nut chewing may induce the upregulation of H19, which serves as a natural sponge for miR-29b and impedes its antifibrotic effects.
Alternative to Poly(2-isopropyl-2-oxazoline) with a Reduced Ability to Crystallize and Physiological LCST
Authors: Wałach Wojciech , Klama-Baryła Agnieszka , Sitkowska Anna , Kowalczuk Agnieszka , Oleszko-Torbus Natalia
Published: February 2021

In this work, we sought to examine whether the presence of alkyl substituents randomly distributed within the main chain of a 2-isopropyl-2-oxazoline-based copolymer will decrease its ability to crystallize when compared to its homopolymer. At the same time, we aimed to ensure an appropriate hydrophilic/lipophilic balance in the copolymer and maintain the phase transition in the vicinity of the human body temperature. For this reason, copolymers of 2-ethyl-4-methyl-2-oxazoline and 2-isopropyl-2-oxazoline were synthesized. The thermoresponsive behavior of the copolymers in water, the influence of salt on the cloud point, the presence of hysteresis of the phase transition and the crystallization ability in a water solution under long-term heating conditions were studied by turbidimetry. The ability of the copolymers to crystallize in the solid state, and their thermal properties, were analyzed by differential scanning calorimetry and X-ray diffractometry. A cytotoxicity assay was used to estimate the viability of human fibroblasts in the presence of the obtained polymers. The results allowed us to demonstrate a nontoxic alternative to poly(2-isopropyl-2-oxazoline) (PiPrOx) with a physiological phase transition temperature (LCST) and a greatly reduced tendency to crystallize. The synthesis of 2-oxazoline polymers with such well-defined properties is important for future biomedical applications.
The Role of Solvents in Lithography-Based Ceramic Manufacturing of Lithium Disilicate
Authors: Hartmann Malte , Pfaffinger Markus , Stampfl Jürgen
Published: February 2021

Digital dentistry is increasingly replacing conventional methods of manually producing dental restorations. With regards to computer-aided manufacturing (CAM), milling is state of the art. Additive manufacturing (AM), as a complementary approach, has also found its way into dental practices and laboratories. Vat photo-polymerization is gaining increasing attention, because it enables the production of full ceramic restorations with high precision. One of the two predominantly used ceramic materials for these applications is lithium disilicate, Li2Si2O5. This glass ceramic exhibits a substantial fracture toughness, although possesses much lower bending strength, than the other predominantly used ceramic material, zirconia. Additionally, it shows a much more natural optical appearance, due to its inherent translucency, and therefore is considered for anterior tooth restorations. In this work, an optimized formulation for photo-reactive lithium disilicate suspensions, to be processed by vat photo-polymerization, is presented. Following the fundamental theoretical considerations regarding this processing technique, a variety of solvents was used to adjust the main properties of the suspension. It is shown that this solvent approach is a useful tool to effectively optimize a suspension with regards to refractive index, rheology, and debinding behavior. Additionally, by examining the effect of the absorber, the exposure time could be reduced by a factor of ten.