bluuebunny/crossref_metadata_2025 huggingface dataset:
Changes in Temperature and Vapor-Pressure Behavior of Bamboo Scrimber in Response to Hot-Pressing Parameters
Authors: Ge Yanglin , Lu Tong , Li Xingong , Hao Xiaofeng , Yang Shoulu , Lu Tonghua , Xu Kang , Li Xianjun
Published: March 2024

This study investigated the heat-transfer behavior of heat-treated and phenolic resin-impregnated bamboo bundle slabs during the hot-pressing process. The significance of these findings lies in their potential to drive advancements in hot-pressing technology, contribute to energy-conservation efforts, and facilitate emission reduction within the bamboo scrimber industry. In this study, the variations in temperature and vapor pressure were investigated during the hot-pressing of bamboo slabs under various conditions, including hot-pressing temperatures (140 °C, 150 °C, 160 °C, and 170 °C), hot-pressing holding times (15 min, 20 min, 25 min, and 30 min), and hot-pressing pressures (4 MPa, 5 MPa, 6 MPa, and 7 MPa). This was achieved using thermocouple sensors and a self-made vapor pressure-monitoring system. The results indicated that higher hot-pressing temperatures significantly increased the heating rate, peak temperature, and core-layer vapor peak pressure of the bamboo bundle slab, with the vapor peak pressure at 170 °C being twice that at 140 °C. Furthermore, extending the holding time had a lesser effect on increasing the peak temperature of the slab but significantly increased the peak vapor pressure in the core layer. Thus, increasing the hot-pressing pressure proved beneficial for slab heating but had a lesser effect on the surface and core-layer peak temperatures. The core-layer vapor pressure of the slab subjected to a hot-press pressure of 7 MPa was 1.8 times higher than that at 4 MPa.
Yeast Bioflavoring in Beer: Complexity Decoded and Built up Again
Authors: Nasuti Chiara , Solieri Lisa
Published: March 2024

Yeast is a powerful bioflavoring platform, suitable to confer special character and complexity to beer aroma. Enhancing yeast bioflavoring represents a chance for the brewing production chain to diversify its product portfolio and to increase environmental sustainability in the era of climate change. In flavor compound metabolism, multiple genes encoding biosynthetic enzymes and the related regulatory factors are still poorly known, but significant advances have been recently made to dissect gene contribution in flavor molecule production. Furthermore, causative mutations responsible for the huge strain diversity in yeast bioflavoring aptitude have been recently disclosed. This review covers the most recent advances in the genetics of yeast bioflavoring, with special regards to higher alcohols, esters, monoterpene alcohols, thiols, and phenolic derivatives of hydroxycinnamic acids. We also critically discussed the most significant strategies to enhance yeast bioflavoring, including bioprospecting for novel Saccharomyces and non-Saccharomyces strains, whole-genome engineering, and metabolic engineering.
Probabilistic Forecasting of Lightning Strikes over the Continental USA and Alaska: Model Development and Verification
Authors: Nikolov Ned , Bothwell Phillip , Snook John
Published: March 2024

Lightning is responsible for the most area annually burned by wildfires in the extratropical region of the Northern Hemisphere. Hence, predicting the occurrence of wildfires requires reliable forecasting of the chance of cloud-to-ground lightning strikes during storms. Here, we describe the development and verification of a probabilistic lightning-strike algorithm running on a uniform 20 km grid over the continental USA and Alaska. This is the first and only high-resolution lightning forecasting model for North America derived from 29-year-long data records. The algorithm consists of a large set of regional logistic equations parameterized on the long-term data records of observed lightning strikes and meteorological reanalysis fields from NOAA. Principal Component Analysis was employed to extract 13 principal components from a list of 611 potential predictors. Our analysis revealed that the occurrence of cloud-to-ground lightning strikes primarily depends on three factors: the temperature and geopotential heights across vertical pressure levels, the amount of low-level atmospheric moisture, and wind vectors. These physical variables isolate the conditions that are favorable for the development of thunderstorms and impact the vertical separation of electric charges in the lower troposphere during storms, which causes the voltage potential between the ground and the cloud deck to increase to a level that triggers electrical discharges. The results from a forecast verification using independent data showed excellent model performance, thus making this algorithm suitable for incorporation into models designed to forecast the chance of wildfire ignitions.
Reformulation of Tunisian Sun-Dried Merguez with Camel Meat: Characterization of Physicochemical and Compositional Changes in Organic Acids, Fatty Acids, Volatile Compounds, and Minerals
Authors: Belguith Khaoula , Jrad Zeineb , Oussaief Olfa , Debara Mohamed , Bouhemda Talel , Sebii Haifa , Hammadi Mohamed , El Hatmi Halima
Published: March 2024

Traditional sun-dried merguez is an authentic Tunisian dried sausage made with a large number of spices and herbs, which was reformulated in this study with camel meat and hump fat and dried as in the artisanal process. This research studied the physicochemical, microbiological, and chemical compositional changes that occurred in fresh camel merguez (FCM) after 12 days of drying to achieve traditional dried camel merguez (DCM). The results showed significant weight loss (54.1%), as well as significant decreases in pH (5.20–4.97), moisture (60.5–12.3%), and water activity (0.986–0.673). These results and the acceptable microbiological quality of DCM can explain the safety of traditionally practiced long-term storage at room temperature. All chemical compositions increased upon drying. The composition of DCM included several organic acids, mainly lactate (2820 mg.kg−1); diverse unsaturated fatty acids, in particular oleic acid (33.2%); and various minerals, specifically iron (8 mg per 100 g), in addition to volatile compounds impacted by herbs and spices rich in terpenes (56.3%). These results can be useful for investing in indigenous products and promoting the exploitation of camel meat.
Optimization of Online Soluble Solids Content Detection Models for Apple Whole Fruit with Different Mode Spectra Combined with Spectral Correction and Model Fusion
Authors: Li Yang , Peng Yankun , Li Yongyu , Yin Tianzhen , Wang Bingwei
Published: March 2024

Soluble solids content (SSC) is one of the main quality indicators of apples, and it is important to improve the precision of online SSC detection of whole apple fruit. Therefore, the spectral pre-processing method of spectral-to-spectral ratio (S/S), as well as multiple characteristic wavelength member model fusion (MCMF) and characteristic wavelength and non-characteristic wavelength member model fusion (CNCMF) methods, were proposed for improving the detection performance of apple whole fruit SSC by diffuse reflection (DR), diffuse transmission (DT) and full transmission (FT) spectra. The modeling analysis showed that the S/S- partial least squares regression models for all three mode spectra had high prediction performance. After competitive adaptive reweighted sampling characteristic wavelength screening, the prediction performance of all three model spectra was improved. The particle swarm optimization–extreme learning machine models of MCMF and CNCMF had the most significant enhancement effect and could make all three mode spectra have high prediction performance. DR, DT, and FT spectra all had some prediction ability for apple whole fruit SSC, with FT spectra having the strongest prediction ability, followed by DT spectra. This study is of great significance and value for improving the accuracy of the online detection model of apple whole fruit SSC.
Hydrolysis of Beef Sarcoplasmic Protein by Dry-Aged Beef-Isolated Penicillium oxalicum and Its Associated Metabolic Pathways
Authors: Liu Yujia , Sun Depeng , Peng Anqi , Li Tingyu , Li Hongmei , Mu Baide , Wang Juan , Cui Mingxun , Piao Chunxiang , Li Guanhao
Published: March 2024

Yanbian cattle have a unique meat flavor, and high-grade meat is in short supply. Therefore, in this study, we aimed to improve the added value of Yanbian cattle low-fat meat and provide a theoretical reference for the subsequent development of an excellent starter. Rump meat from Yanbian cattle was dry-aged and then screened for protease-producing fungi. Three protease-producing fungi (Yarrowia hollandica (D4 and D11), Penicillium oxalicum (D5), and Meesziomyces ophidis (D20)) were isolated from 40 d dry-aged beef samples, and their ability to hydrolyze proteins was determined using bovine sarcoplasmic protein extract. SDS-PAGE showed that the ability of Penicillium oxalicum (D5) to degrade proteins was stronger than the other two fungi. In addition, the volatile component content of sarcoplasmic proteins in the D5 group was the highest (45.47%) and comprised the most species (26 types). Metabolic pathway analysis of the fermentation broth showed that phenylalanine, tyrosine, and tryptophan biosynthesis was the most closely related metabolic pathway in sarcoplasmic protein fermentation by Penicillium oxalicum (D5). Dry-aged beef-isolated Penicillium oxalicum serves as a potential starter culture for the fermentation of meat products.
Impact of Molecular Weight Variations in Dendrobium officinale Polysaccharides on Antioxidant Activity and Anti-Obesity in Caenorhabditis elegans
Authors: Pang Xiao , Wang Heqin , Guan Chunbo , Chen Qiufeng , Cui Xinwen , Zhang Xiuqing
Published: March 2024

This research investigates the impact of Dendrobium officinale polysaccharides (DOP) with different molecular weights on antioxidant effects, lifespan enhancement, and obesity reduction, utilizing both in vitro analyses and the Caenorhabditis elegans (C. elegans) model. Through a series of experiments—ranging from the extraction and modification of polysaccharides, Gel Permeation Chromatography (GPC), and analysis of composition to the evaluation of antioxidant capabilities, this study thoroughly examines DOP and its derivatives (DOP5, DOP15, DOP25) produced via H2O2-Fe2+ degradation. The results reveal a direct relationship between the molecular weight of polysaccharides and their bioactivity. Notably, DOP5, with its intermediate molecular weight, demonstrated superior antioxidant properties, significantly extended the lifespan, and improved the health of C. elegans. Furthermore, DOP15 appeared to regulate lipid metabolism by affecting crucial lipid metabolism genes, including fat-4, fat-5, fat-6, sbp-1, and acs-2. These findings highlight the potential application of DOP derivatives as natural antioxidants and agents against obesity, contributing to the development of functional foods and dietary supplements.
Lactic Acid Bacteria of Marine Origin as a Tool for Successful Shellfish Farming and Adaptation to Climate Change Conditions
Authors: Čanak Iva , Kostelac Deni , Jakopović Željko , Markov Ksenija , Frece Jadranka
Published: March 2024

Climate change, especially in the form of temperature increase and sea acidification, poses a serious challenge to the sustainability of aquaculture and shellfish farming. In this context, lactic acid bacteria (LAB) of marine origin have attracted attention due to their ability to improve water quality, stimulate the growth and immunity of organisms, and reduce the impact of stress caused by environmental changes. Through a review of relevant research, this paper summarizes previous knowledge on this group of bacteria, their application as protective probiotic cultures in mollusks, and also highlights their potential in reducing the negative impacts of climate change during shellfish farming. Furthermore, opportunities for further research and implementation of LAB as a sustainable and effective solution for adapting mariculture to changing climate conditions were identified.
Sustainable Valorization of Industrial Cherry Pomace: A Novel Cascade Approach Using Pulsed Electric Fields and Ultrasound Assisted-Extraction
Authors: Rrucaj Ervehe , Carpentieri Serena , Scognamiglio Mariarosa , Siano Francesco , Ferrari Giovanna , Pataro Gianpiero
Published: March 2024

In this study, a two-stage cascade extraction process utilizing pulsed electric fields (PEF) (3 kV/cm, 10 kJ/kg) for initial extraction, followed by ultrasound (US) (200 W, 20 min)-assisted extraction (UAE) in a 50% (v/v) ethanol-water mixture (T = 50 °C, t = 60 min), was designed for the efficient release of valuable intracellular compounds from industrial cherry pomace. The extracted compounds were evaluated for total phenolic content (TPC), flavonoid content (FC), total anthocyanin content (TAC), and antioxidant activity (FRAP), and were compared with conventional solid-liquid extraction (SLE). Results showed that the highest release of bioactive compounds occurred in the first stage, which was attributed to the impact of PEF pre-treatment, resulting in significant increases in TPC (79%), FC (79%), TAC (83%), and FRAP values (80%) of the total content observed in the post-cascade PEF-UAE process. The integration of UAE into the cascade process further augmented the extraction efficiency, yielding 21%, 49%, 56%, and 26% increases for TPC, FC, TAC, and FRAP, respectively, as compared to extracts obtained through a second-stage conventional SLE. HPLC analysis identified neochlorogenic acid, 4-p-coumaroylquinic, and cyanidin-3-O-rutinoside as the predominant phenolic compounds in both untreated and cascade-treated cherry pomace extracts, and no degradation of the specific compounds occurred upon PEF and US application. SEM analysis revealed microstructural changes in cherry pomace induced by PEF and UAE treatments, enhancing the porosity and facilitating the extraction process. The study suggests the efficiency of the proposed cascade PEF-UAE extraction approach for phenolic compounds from industrial cherry pomace with potential applications to other plant-based biomasses.
Supercritical CO2 Treatment to Modify Techno-Functional Properties of Proteins Extracted from Tomato Seeds
Authors: Mateo-Roque Paola , Morales-Camacho Jocksan I. , Jara-Romero Guadalupe Janet , Rosas-Cárdenas Flor de Fátima , Huerta-González Luis , Luna-Suárez Silvia
Published: March 2024

Tomato seeds are a rich source of protein that can be utilized for various industrial food purposes. This study delves into the effects of using supercritical CO2 (scCO2) on the structure and techno-functional properties of proteins extracted from defatted tomato seeds. The defatted meal was obtained using hexane (TSMH) and scCO2 (TSMC), and proteins were extracted using water (PEWH and PEWC) and saline solution (PESH and PESC). The results showed that scCO2 treatment significantly improved the techno-functional properties of protein extracts, such as oil-holding capacity and foaming capacity (especially for PEWC). Moreover, emulsifying capacity and stability were enhanced for PEWC and PESC, ranging between 4.8 and 46.7% and 11.3 and 96.3%, respectively. This was made possible by the changes in helix structure content induced by scCO2 treatment, which increased for PEWC (5.2%) and decreased for PESC (8.0%). Additionally, 2D electrophoresis revealed that scCO2 hydrolyzed alkaline proteins in the extracts. These findings demonstrate the potential of scCO2 treatment in producing modified proteins for food applications.
Evolving Patterns in Irrational Numbers Using Waiting Times between Digits
Authors: Ogunjo Samuel , Kantz Holger
Published: March 2024

There is an increasing interest in determining if there exist observable patterns or structures within the digits of irrational numbers. We extend this search by investigating the interval in position between two consecutive occurrences of the same digit, a kind of waiting time statistics. We characterise these by the burstiness measure which distinguishes if the inter-event times are periodic, bursty, or Poisson processes. Furthermore, the complexity–entropy plane was used to determine if the intervals are stochastic or chaotic. We analyse sequences of the first 1 million digits of the numbers π, e, 2, and ϕ. We find that the intervals between single, double, and triple digits are Poisson processes with a burstiness measure in the range −0.05≤B≤0.05 for the four numbers studied. This result is supported by a complexity–entropy plane analysis, which shows that the time intervals have the same characteristics as Gaussian noise. The four irrational numbers have identical degrees of complexity and burstiness in their inter-event analysis.
The Impact of Ar or N2 Atmosphere on the Structure of Bi-Fe-Carbon Xerogel Based Composites as Electrode Material for Detection of Pb2+ and H2O2
Authors: Fort Carmen I. , Rusu Mihai M. , Cotet Liviu C. , Vulpoi Adriana , Todea Milica , Baia Monica , Baia Lucian
Published: March 2024

In this study, bismuth- and iron-embedded carbon xerogels (XG) were obtained using a modified resorcinol formaldehyde sol–gel synthesis method followed by additional enrichment with iron content. Pyrolysis treatment was performed at elevated temperatures under Ar or N2 atmosphere to obtain nanocomposites with different reduction yields (XGAr or XGN). The interest was focused on investigating the extent to which changes in the pyrolysis atmosphere of these nanocomposites impact the structure, morphology, and electrical properties of the material and consequently affect the electroanalytical performance. The structural and morphological particularities derived from X-ray diffraction (XRD), Raman spectroscopy, scanning electron microscopy (SEM), transmission electron microscopy (TEM), and X-ray photoelectron spectroscopy (XPS) measurements revealed the formation of the nanocomposite phases, mostly metal/oxide components. The achieved performances for the two modified electrodes based on XG treated under Ar or N2 atmosphere clearly differ, as evidenced by the electroanalytical parameters determined from the detection of heavy metal cations (Pb2+) or the use of the square wave voltammetry (SWV) technique, biomarkers (H2O2), or amperometry. By correlating the differences obtained from electroanalytical measurements with those derived from morphological, structural, and surface data, a few utmost important aspects were identified. Pyrolysis under Ar atmosphere favors a significant increase in the α-Fe2O3 amount and H2O2 detection performance (sensitivity of 0.9 A/M and limit of detection of 0.17 μM) in comparison with pyrolysis under N2 (sensitivity of 0.5 A/M and limit of detection of 0.36 μM), while pyrolysis under N2 atmosphere leads to an increase in the metallic Bi amount and Pb2+ detection performance (sensitivity of 8.44 × 103 A/M and limit of detection of 33.05 pM) in comparison with pyrolysis under Ar (sensitivity of 6.47·103 A/M and limit of detection of 46.37 pM).
In Vivo Evaluation of Wound Healing Efficacy of Gel-Based Dressings Loaded with Pycnogenol™ and Ceratothoa oestroides Extracts
Authors: Vitsos Andreas , Ieronymaki Dimitra , Kostaki Maria , Almpani Chara , Barda Christina , Kikionis Stefanos , Sfiniadakis Ioannis , Dallas Paraskevas , Rallis Michail Christou
Published: March 2024

Ceratothoa oestroides and French maritime pine bark (Pycnogenol™) extracts are considered promising therapeutic agents in wound healing. This study explores the healing efficacy of composite dressings containing these extracts, aiming to enhance their stability and effectiveness, utilizing a low-temperature vacuum method for producing Sodium Alginate—Maltodextrin gel dressings. Surgical wounds were inflicted on SKH-hr2 hairless mice. Dressings were loaded with Pycnogenol™ and/or C. oestroides extracts and assessed for their efficacy. Wound healing was primarily evaluated by clinical and histopathological evaluation and secondarily by Antera 3D camera and biophysical measurements. Dressings were stable and did not compromise the therapeutic properties of C. oestroides extract. All interventions were compared to the C. oestroides ointment as a reference product. Most of the wounds treated with the reference formulation and the C. oestrodes dressing had already closed by the 15th day, with histological scores of 7 and 6.5, respectively. In contrast, wounds treated with Pycnogenol™, either alone or in combination with C. oestroides, did not close by the end of the experiment (16th day), with histological scores reaching 15 in both cases. Furthermore, treatment with 5% Pycnogenol™ dressing appeared to induce skin thickening and increase body temperature. The study underscores the wound healing potential of C. oestroides extracts and highlights the need for further research to optimize Pycnogenol™ dosing in topical applications.
A Case Study of a Rare Undifferentiated Spindle Cell Sarcoma of the Penis: Establishment and Characterization of Patient-Derived Models
Authors: Sousa Ariane Cavalcante dos Santos , Fernandes Bruno Leonardo Nascimento Correa , da Silva Jeronimo Paulo Assis , Stevanato Filho Paulo Roberto , Coimbra Luiza Bitencourt de Carvalho Terci , de Oliveira Beserra Adriano , Alvarenga Ana Luiza , Maida Giovanna , Guimaraes Camila Tokumoto , Nakamuta Ingrid Martinez , Marchi Fabio Albuquerque , Alves Camila , Lichtenfels Martina , de Farias Caroline Brunetto , Kupper Bruna Elisa Catin , Costa Felipe D’Almeida , de Mello Celso Abdon Lopes , Carraro Dirce Maria , Torrezan Giovana Tardin , Lopes Ademar , dos Santos Tiago Goss
Published: March 2024

Rare sarcomas present significant treatment challenges compared to more prevalent soft tissue sarcomas due to limited treatment options and a poor understanding of their biology. This study investigates a unique case of penile sarcoma, providing a comprehensive morphological and molecular analysis. Through the creation of experimental patient-derived models—including patient-derived xenograft (PDX), 3D, and monolayer primary cultures—we successfully replicated crucial molecular traits observed in the patient’s tumor, such as smooth muscle actin and CD99 expression, along with specific mutations in genes like TSC2 and FGFR4. These models are helpful in assessing the potential for an in-depth exploration of this tumor’s biology. This comprehensive approach holds promise in identifying potential therapeutic avenues for managing this exceedingly rare soft tissue sarcoma.
Augmented Articulating Spacers in Infected Total Knee Arthroplasty: Surgical Technique
Authors: De Mauro Domenico , Festa Enrico , Di Gennaro Donato , Ascione Tiziana , Coletta Giannantonio , Mariconda Massimo , Balato Giovanni
Published: March 2024

Periprosthetic joint infections (PJIs) are a prominent subject of discussion in orthopedics and are frequently debated at conferences and congresses. In the context of PJIs affecting the knee, the decision between following a one-stage or two-stage treatment approach has historically been a pivotal consideration. The first option is limited by indications and potentially devastating complications in case of failure, whereas the second is widely accepted as the gold standard. Initially, the spacer was conceived solely to restore and maintain knee space after removal of the implant. An articulating spacer was introduced to mitigate patient limitations and improve knee function and quality of life. Two main types of articulating spacers are utilized in knee PJI treatment: the mold spacer and the metal-on-poly spacer. This text outlines a technique for metal-on-poly spacer implants. Based on our experience and the existing literature, this approach facilitates early full weight bearing and faster recovery of the knee’s range of motion, ultimately improving the quality of life after surgery, thus allowing the spacer retention for an extended period, as suggested by the 1.5-stage revision.
Recognition of Early Cardiovascular Disease Symptoms in Hypertensive and Dyslipidemic Individuals of Icheon, Korea: Insights into Educational Levels and Health Literacy
Authors: Lee Jeehye , Ryu Dong-Hee
Published: March 2024

The study aimed to explore the relationship between the presence of hypertension or dyslipidemia and the recognition of early symptoms of cardiovascular diseases (CVD), particularly acute myocardial infarction (AMI) and stroke. It is crucial for individuals with hypertension or dyslipidemia to recognize early symptoms of AMI and stroke, as timely and appropriate intervention can lead to favorable health outcomes. The study enrolled 104 participants aged 19 and above who are current residents of the Icheon region, Gyeonggi, Korea. The assessment of early symptoms of AMI and stroke utilized adapted items from the Korea Community Health Survey. In consideration of health literacy and education attainment, logistic regression analyses were conducted. While there was no significant association between hypertension and awareness of AMI or stoke symptoms, individuals with dyslipidemia demonstrated enhanced recognition of specific AMI symptoms, such as ‘sudden chest pain or pressure’ and ‘sudden feeling of breathlessness’. No significant associations were observed between hypertension or dyslipidemia and awareness of stroke symptoms. The study emphasized the significance of targeted health education programs for individuals with chronic conditions to enhance their awareness of early symptoms of AMI and stroke.
Winds of Change: Urgent Challenges and Emerging Opportunities in Submerged Prehistory, a Perspective from the North Sea
Authors: Walker James , Gaffney Vincent , Harding Rachel , Fraser Andrew , Fitch Simon , Boothby Victoria
Published: March 2024

Development of the continental shelf has accelerated significantly as nations around the world seek to harness offshore renewable energy. Many areas marked for development align with submerged palaeolandscapes. Poorly understood and difficult to protect, these vulnerable, prehistoric landscapes provide specific challenges for heritage management. Indeed, there now appears to be a schism between what underwater cultural heritage policy intends and what it is achieving in practice. Shortcomings in international and national legislature ensures that large parts of the continental shelf, including areas under development, may have little or no legal protection. Increasingly impacted by extensive development, these unique cultural landscapes are ever more at risk. However, heritage challenges posed by such development also create opportunities. An immense amount of data is being generated by development, and there is an opportunity to establish broader cooperative relationships involving industrial stakeholders, national curators, government bodies, and heritage professionals. As a matter of urgency, the archaeological community must better engage with the offshore sector and development process. If achieved, we may revolutionise our knowledge of submerged prehistoric settlement and land use. Otherwise, our capacity to reconstruct prehistoric settlement patterns, learn from past climate change, or simply manage what are among the best-preserved postglacial landscapes globally may be irreparably undermined.
Farmers’ Knowledge, Perceptions, and Management Practices of False Codling Moth (Thaumatotibia leucotreta) in Smallholder Capsicum sp. Cropping Systems in Kenya
Authors: Onamu Emmanuel M. , Mutyambai Daniel M. , Nyangau Isaac M. , Nderitu John H. , Kasina Muo , Kilalo Dorah C. , Mweke Allan N.
Published: March 2024

False codling moth (FCM) Thaumatotibia leucotreta Meyrick (Lepidoptera: Tortricidae) is currently the main pest of phytosanitary concern in international trade, causing rejection and decline of horticultural produce from Kenya exported to the European Union (EU). Overreliance on synthetic insecticides to control this pest is ineffective and unsustainable in the long run, whereas continuous use of pesticides results in high levels of residues in the produce. To gather farmers’ knowledge, attitudes, and practices used by smallholder farmers to manage this pest, a field survey was carried out in 10 Capsicum sp. (Solanales: Solanaceae)-producing counties in Kenya. Data were collected using semi-structured questionnaires administered through face-to-face interviews and focus group discussions involving 108 individual farmers, 20 key informants, and 10 focus group discussions. The majority of the respondents (83.33%) were aware of the FCM infesting Capsicum sp. About three quarters of the farmers (76.85%) reported yield losses and unmarketable quality of FCM-infested Capsicum sp. Most farmers interviewed (99.07%) used insecticides as a management tool. In contrast, only 39.81% of the farmers applied integrated pest management strategies including use of biological control agents and intercropping with repellent plants to control this pest. The results show that FCM is perceived as a significant threat to the horticultural industry of Kenya. Training needs for smallholder farmers and key informants to avoid overreliance on synthetic chemical pesticides and to maintain export goals to the EU where identified.
Exploring the PpEXPs Family in Peach: Insights into Their Role in Fruit Texture Development through Identification and Transcriptional Analysis
Authors: Guo Yakun , Song Conghao , Gao Fan , Zhi Yixin , Zheng Xianbo , Wang Xiaobei , Zhang Haipeng , Hou Nan , Cheng Jun , Wang Wei , Zhang Langlang , Ye Xia , Li Jidong , Tan Bin , Lian Xiaodong , Feng Jiancan
Published: March 2024

Expansins (EXPs) loosen plant cell walls and are involved in diverse developmental processes through modifying cell-walls; however, little is known about the role of PpEXPs in peach fruit. In this study, 26 PpEXP genes were identified in the peach genome and grouped into four subfamilies, with 20 PpEXPAs, three PpEXPBs, one PpEXPLA and two PpEXPLBs. The 26 PpEXPs were mapped on eight chromosomes. The primary mode of gene duplication of the PpEXPs was dispersed gene duplication (DSD, 50%). Notably, cis-elements involved in light responsiveness and MeJA-responsiveness were detected in the promoter regions of all PpEXPs, while ethylene responsive elements were observed in 12 PpEXPs. Transcript profiling of PpEXPs in the peach fruit varieties of MF (melting), NMF (non-melting) and SH (stony hard) at different stages showed that PpEXPs displayed distinct expression patterns. Among the 26 PpEXPs, 15 PpEXPs were expressed in the fruit. Combining the expressing patterns of PpEXPs in fruits with different flesh textures, PpEXPA7, PpEXPA13 and PpEXPA15 were selected as candidate genes, as they were highly consistent with the patterns of previous reported key genes (PpPGM, PpPGF and PpYUC11) in regard to peach fruit texture. The genes with different expression patterns between MF and NMF were divided into 16 modules, of which one module, with pink and midnightblue, negatively correlated with the phenotype of fruit firmness and was identified as PpEXPA1 and PpEXPA7, while the other module was identified as PpERF in the pink module, which might potentially effect fruit texture development by regulating PpEXPs. These results provide a foundation for the functional characterization of PpEXPs in peach.
Biological Control of Pseudomonas syringae in Tomato Using Filtrates and Extracts Produced by Alternaria leptinellae
Authors: García-Latorre Carlos , Rodrigo Sara , Santamaria Oscar
Published: March 2024

Endophytic fungi offer promising alternatives for sustainable plant disease management strategies, often through the production of bioactive secondary metabolites. This study investigated the biocontrol potential of filtrates and extracts, produced under controlled conditions, from Alternaria leptinellae E138 against Pseudomonas syringae in tomato plants under greenhouse conditions. To understand the main mechanisms involved in biocontrol, the direct inhibition of bacterial growth and disruption of quorum sensing activity caused by metabolites were studied in vitro, as well as indirect mechanisms, such as their capacity to produce phytohormone-like substances, nutrient mobilization, and antioxidant activity, which can enhance plant growth and fitness. Moreover, a mass spectrometry analysis was used to tentatively identify the secondary metabolites present in the extract with antimicrobial properties, which could explain the biocontrol effects observed. Mycopriming assays, involving the direct treatment of tomato seeds with the fungal A. leptinellae E138 extracts, produced increased germination rates and seedling vigor in tomato seeds. As another treatment, postemergence application of the extracts in greenhouse conditions significantly improved plant health and resulted in a 41% decrease in disease severity. Overall, this study underscores the potential of A. leptinellae E138 extract as a plant growth promoter with biocontrol capabilities, offering promising avenues for sustainable plant disease management.
Determining Moisture Content of Basil Using Handheld Near-Infrared Spectroscopy
Authors: Gorji Reyhaneh , Skvaril Jan , Odlare Monica
Published: March 2024

Accurate and rapid determination of moisture content is essential in crop production and decision-making for irrigation. Near-infrared (NIR) spectroscopy has been shown to be a promising method for determining moisture content in various agricultural products, including herbs and vegetables. This study tested the hypothesis that NIR spectroscopy is effective in accurately measuring the moisture content of Genovese basil (Ocimum basilicum L.), with the objective of developing a respective calibration model. Spectral data were obtained from a total of 120 basil leaf samples over a period of six days. These included freshly harvested and detached leaves, as well as those left in ambient air for 1–6 days. Five spectra were taken from each leaf using a handheld NIR spectrophotometer, which covers the first and second overtones of the NIR spectral region: 950–1650 nm. After the spectral acquisition, the leaves were weighed for fresh mass and then put in an oven for 72 h at 80 °C to determine the dry weight and calculate the reference moisture content. The calibration model was developed using multivariate analysis in MATLAB, including preprocessing and regression modeling. The data obtained from 75% of the samples were used for model training and 25% for validation. The final model demonstrates strong performance metrics. The root mean square error of calibration (RMSEC) is 2.9908, the root mean square error of cross-validation (RMSECV) is 3.2368, and the root mean square error of prediction (RMSEP) reaches 2.4675. The coefficients of determination for calibration (R2C) and cross-validation (R2CV) are consistent, with values of 0.829 and 0.80, respectively. The model’s predictive ability is indicated by a coefficient of determination for prediction (R2P) of 0.86. The range error ratio (RER) stands at 11.045—highlighting its predictive performance. Our investigation, using handheld NIR spectrophotometry, confirms NIR’s usefulness in basil moisture determination. The rapid determination offers valuable insights for irrigation and crop management.
Near-Freezing Temperature Storage Improves Peach Fruit Chilling Tolerance by Regulating the Antioxidant and Proline Metabolism
Authors: Zhao Handong , Meng Shuqi , Fu Maorun , Chen Qingmin
Published: March 2024

Chilling injury (CI) in peach fruit (Prunus persica cv. Yuhualu) is generally caused by long-time low temperature (5 °C or 0 °C) storage. However, peach fruit stored at near-freezing temperature (NFT in this research is −1 °C), defined as within 0.5 °C above the biological freezing point of biological tissue, does not exhibit CI symptoms. The effect of NFT on the CI, proline metabolism, and antioxidant capability of peach fruit during storage was studied and compared with 5 °C and 0 °C storage as controls. The results exhibit that NFT completely inhibited the occurrence of CI in peach fruit. NFT significantly (p < 0.05) enhanced the activities of superoxide dismutase, catalase, ascorbate peroxidase, and 1,1-diphenyl-2-picrylhydrazyl scavenging capacity. Moreover, the increase of malondialdehyde, ion leakage, and H2O2 accumulation were inhibited remarkably by NFT, and decreases in the contents of phenolics and ascorbic acid were slowed significantly in peach fruit stored at NFT (p < 0.05). Additionally, NFT storage enhanced proline accumulation by modulating the activity of proline metabolizing enzymes. In conclusion, the above results suggest that NFT storage can improve the chilling tolerance of peach fruit by regulating the antioxidant defense and proline metabolism, which might represent a potential novel method to store fruits and vegetables for longer storage times.
A Systematic Umbrella Review of the Effects of Teledentistry on Costs and Oral-Health Outcomes
Authors: Scheerman Janneke F. M. , Qari Alaa H. , Varenne Benoit , Bijwaard Harmen , Swinckels Laura , Giraudeau Nicolas , van Meijel Berno , Mariño Rodrigo
Published: March 2024

Teledentistry offers possibilities for improving efficiency and quality of care and supporting cost-effective healthcare systems. This umbrella review aims to synthesize existing systematic reviews on teledentistry and provide a summary of evidence of its clinical- and cost-effectiveness. A comprehensive search strategy involving various teledentistry-related terms, across seven databases, was conducted. Articles published until 24 April 2023 were considered. Two researchers independently reviewed titles, abstracts and full-text articles. The quality of the included reviews was critically appraised with the AMSTAR-2 checklist. Out of 749 studies identified, 10 were included in this umbrella review. Two reviews focusing on oral-health outcomes revealed that, despite positive findings, there is not yet enough evidence for the long-term clinical effectiveness of teledentistry. Ten reviews reported on economic evaluations or costs, indicating that teledentistry is cost-saving. However, these conclusions were based on assumptions due to insufficient evidence on cost-effectiveness. The main limitation of our umbrella review was the critically low quality of the included reviews according to AMSTAR-2 criteria, with many of these reviews basing their conclusions on low-quality studies. This highlights the need for high-quality experimental studies (e.g., RCTs, factorial designs, stepped-wedge designs, SMARTs and MRTs) to assess teledentistry’s clinical- and cost-effectiveness.
Correction: Siegel et al. PEN-13: A New Generic 13-Item Questionnaire for Measuring Patient Enablement (German Version). Int. J. Environ. Res. Public Health 2019, 16, 4867
Authors: Siegel Achim , Ehmann Anna T. , Meyer Ingo , Gröne Oliver , Niebling Wilhelm , Martus Peter , Rieger Monika A.
Published: March 2024

In the original publication [...]
A Community-Based Prostate Cancer Screening and Education Program for Asian American Men in Medically Underserved Communities
Authors: Cho Dalnim , Gor Beverly , Hwang Hyunsoo , Wang Xuemei , Hernandez Mike , Jones Lovell A. , Frost Jacqueline , Roberson Pamela , Pettaway Curtis A.
Published: March 2024

This study analyzed data from a community-based prostate cancer (PCa) education and screening program (Prostate Outreach Project; POP) to enhance PCa-related knowledge among medically underserved Asian American men. It also examined PCa screening history, clinical abnormalities based on prostate-specific antigen (PSA) tests and digital rectal examination (DRE) results, and follow-up and PCa diagnosis rates. Participants—521 Asian men (251 Vietnamese, 142 Chinese, and 128 South Asians)—were offered PCa screening using PSA tests and/or DRE and an educational session on PCa. Of these men, 277 completed PCa-related knowledge surveys before and after viewing an educational video. Significant between-group differences in PCa-related knowledge were found at pre-assessment (p < 0.001) but not at post-assessment (p = 0.11), at which time all groups showed improved PCa-related knowledge. Most participants (77.9%) had never received PCa screening, but Vietnamese men had the lowest previous screening rate (17.3%). Chinese men had elevated PSA values and the highest abnormal DRE rates. Of the 125 men with abnormal screening outcomes, only 15.2% had adequate follow-up. Of the 144 men diagnosed with PCa in POP, 11.1% were Asians (seven Chinese, six Vietnamese, and three South Asian). Despite the ethnic heterogeneity among Asian men, a community outreach program may successfully enhance their PCa-related knowledge.
Mapping Street Patterns with Network Science and Supervised Machine Learning
Authors: Wu Cai , Wang Yanwen , Wang Jiong , Kraak Menno-Jan , Wang Mingshu
Published: March 2024

This study introduces a machine learning-based framework for mapping street patterns in urban morphology, offering an objective, scalable approach that transcends traditional methodologies. Focusing on six diverse cities, the research employed supervised machine learning to classify street networks into gridiron, organic, hybrid, and cul-de-sac patterns with the street-based local area (SLA) as the unit of analysis. Utilising quantitative street metrics and GIS, the study analysed the urban form through the random forest method, which reveals the predictive features of urban patterns and enables a deeper understanding of the spatial structures of cities. The findings showed distinctive spatial structures, such as ring formations and urban cores, indicating stages of urban development and socioeconomic narratives. It also showed that the unit of analysis has a major impact on the identification and study of street patterns. Concluding that machine learning is a critical tool in urban morphology, the research suggests that future studies should expand this framework to include more cities and urban elements. This would enhance the predictive modelling of urban growth and inform sustainable, human-centric urban planning. The implications of this study are significant for policymakers and urban planners seeking to harness data-driven insights for the development of cities.
Effects of Anti-Fibrotic Drugs on Transcriptome of Peripheral Blood Mononuclear Cells in Idiopathic Pulmonary Fibrosis
Authors: Ishii Daisuke , Kawasaki Takeshi , Sato Hironori , Tatsumi Koichiro , Imamoto Takuro , Yoshioka Keiichiro , Abe Mitsuhiro , Hasegawa Yoshinori , Ohara Osamu , Suzuki Takuji
Published: March 2024

Two anti-fibrotic drugs, pirfenidone (PFD) and nintedanib (NTD), are currently used to treat idiopathic pulmonary fibrosis (IPF). Peripheral blood mononuclear cells (PBMCs) are immunocompetent cells that could orchestrate cell–cell interactions associated with IPF pathogenesis. We employed RNA sequencing to examine the transcriptome signature in the bulk PBMCs of patients with IPF and the effects of anti-fibrotic drugs on these signatures. Differentially expressed genes (DEGs) between “patients with IPF and healthy controls” and “before and after anti-fibrotic treatment” were analyzed. Enrichment analysis suggested that fatty acid elongation interferes with TGF-β/Smad signaling and the production of oxidative stress since treatment with NTD upregulates the fatty acid elongation enzymes ELOVL6. Treatment with PFD downregulates COL1A1, which produces wound-healing collagens because activated monocyte-derived macrophages participate in the production of collagen, type I, and alpha 1 during tissue damage. Plasminogen activator inhibitor-1 (PAI-1) regulates wound healing by inhibiting plasmin-mediated matrix metalloproteinase activation, and the inhibition of PAI-1 activity attenuates lung fibrosis. DEG analysis suggested that both the PFD and NTD upregulate SERPINE1, which regulates PAI-1 activity. This study embraces a novel approach by using RNA sequencing to examine PBMCs in IPF, potentially revealing systemic biomarkers or pathways that could be targeted for therapy.
MicroRNA Associations with Preterm Labor—A Systematic Review
Authors: Kondracka Adrianna , Stupak Aleksandra , Rybak-Krzyszkowska Magda , Kondracki Bartosz , Oniszczuk Anna , Kwaśniewska Anna
Published: March 2024

This systematic review delves into the connections between microRNAs and preterm labor, with a focus on identifying diagnostic and prognostic markers for this crucial pregnancy complication. Covering studies disseminated from 2018 to 2023, the review integrates discoveries from diverse pregnancy-related scenarios, encompassing gestational diabetes, hypertensive disorders and pregnancy loss. Through meticulous search strategies and rigorous quality assessments, 47 relevant studies were incorporated. The synthesis highlights the transformative potential of microRNAs as valuable diagnostic tools, offering promising avenues for early intervention. Notably, specific miRNAs demonstrate robust predictive capabilities. In conclusion, this comprehensive analysis lays the foundation for subsequent research, intervention strategies and improved outcomes in the realm of preterm labor.
Substance P Concentration in Gestational Diabetes and Excessive Gestational Weight Gain and Its Impact on Neonatal Anthropometry
Authors: Niebrzydowska-Tatus Magdalena , Pełech Aleksandra , Bień Katarzyna , Rekowska Anna K. , Domańska Aleksandra , Kimber-Trojnar Żaneta , Leszczyńska-Gorzelak Bożena , Trojnar Marcin
Published: March 2024

Fetal programming is a process initiated by intrauterine conditions, leaving a lasting impact on the offspring’s health, whether they manifest immediately or later in life. It is believed that children born to mothers with gestational diabetes mellitus (GDM) and excessive gestational weight gain (EGWG) may be at an increased risk of developing type 2 diabetes mellitus (T2DM) and obesity later in their adult lives. Substance P is a neurotransmitter associated with obesity development and impairment of insulin signaling. Dysregulation of substance P could lead to several pregnancy pathologies, such as preeclampsia and preterm birth. Our study aimed to compare substance P concentrations in serum and umbilical cord blood in patients with GDM, EGWG, and healthy women with a family history of gestational weight gain. Substance P levels in umbilical cord blood were significantly higher in the GDM group compared to the EGWG and control groups. Substance P levels in serum and umbilical cord blood were positively correlated in all groups and the GDM group. A very interesting direction for future research is the relationship between the concentration of substance P in newborns of diabetic mothers and the occurrence of respiratory distress syndrome as a complication of impaired surfactant synthesis. To our knowledge, it is the first study assessing substance P concentration in GDM and EGWG patients.
Approach to the “Missing” Diarylsilylene: Formation, Characterization, and Intramolecular C–H Bond Activation of Blue Diarylsilylenes with Bulky Rind Groups
Authors: Mochihara Kazuki , Morimoto Tatsuto , Ota Kei , Marumoto Shinsuke , Hashizume Daisuke , Matsuo Tsukasa
Published: March 2024

The treatment of the bulky Rind-based dibromosilanes, (Rind)2SiBr2 (2) [Rind = 1,1,7,7-tetra-R1-3,3,5,5-tetra-R2-s-hydrindacen-4-yl: EMind (a: R1 = Et, R2 = Me) and Eind (b: R1 = R2 = Et)], with two equivalents of tBuLi in Et2O at low temperatures resulted in the formation of blue solutions derived from the diarylsilylenes, (Rind)2Si: (3). Upon warming the solutions above −20 °C, the blue color gradually faded, accompanying the decomposition of 3 and yielding cyclic hydrosilanes (4) via intramolecular C–H bond insertion at the Si(II) center. The molecular structures of the bulky Eind-based 3b and 4b were confirmed by X-ray crystallography. Thus, at −20 °C, blue crystals were formed (Crystal-A), which were identified as mixed crystals of 3b and 4b. Additionally, colorless crystals of 4b as a singular component were isolated (Crystal-B), whose structure was also determined by an X-ray diffraction analysis. Although the isolation of 3 was difficult due to their thermally labile nature, their structural characteristics and electronic properties were discussed based on the experimental findings complemented by computational results. We also examined the hydrolysis of 3b to afford the silanol, (Eind)2SiH(OH) (5b).