bluuebunny/crossref_metadata_2025 huggingface dataset:
The Response of Carbon Stocks to Land Use/Cover Change and a Vulnerability Multi-Scenario Analysis of the Karst Region in Southern China Based on PLUS-InVEST
Authors: Du Shuanglong , Zhou Zhongfa , Huang Denghong , Zhang Fuxianmei , Deng Fangfang , Yang Yue
Published: November 2023

Quantitatively revealing the response of carbon stocks to land use change (LUCC) and analyzing the vulnerability of ecosystem carbon stock (ECS) services are of great significance for maintaining the carbon cycle and ecological security. For this study, China’s Guizhou Province was the study area. Land use data in 2000, 2010, and 2020 were selected to explore the impacts of LUCC on carbon stocks in multiple scenarios by combining the PLUS and InVEST models and then analyzing the vulnerability of ECS services. The results show that forest land plays an important role in improving ECS services in karst plateau mountainous areas. In 2000–2020, forest land expansion offset the carbon stock reduced by the expansion of built-up land, greatly improving the regional ECS function. Following the natural trend (NT), the total carbon stock in Guizhou Province will decrease by 1.86 Tg; however, under ecological protection (EP) measures, the ECS service performs a positive function for LUCC. Focusing on socioeconomic development (ED) will increase the vulnerability of the regional ECS service. In the future, the forest land area size should be increased, and built-up land should be restricted to better improve the service function of ECS in karst plateau mountainous areas.
Supporting Operational Tree Marking Activities through Airborne LiDAR Data in the Frame of Sustainable Forest Management
Authors: Georgopoulos Nikos , Stefanidou Alexandra , Gitas Ioannis Z.
Published: November 2023

Implementing adaptation and mitigation strategies in forest management constitutes a primary tool for climate change mitigation. To the best of our knowledge, very little research so far has examined light detection and ranging (LiDAR) technology as a decision tool for operational cut-tree marking. This study focused on investigating the potential of airborne LiDAR data in enhancing operational tree marking in a dense, multi-layered forest over complex terrain for actively supporting long-term sustainable forest management. A detailed tree registry and density maps were produced and evaluated for their accuracy using field data. The derived information was subsequently employed to estimate additional tree parameters (e.g., biomass and tree-sequestrated carbon). An integrated methodology was finally proposed using the developed products for supporting the time- and effort-efficient operational cut-tree marking. The results showcased the low detection ability (R2 = 0.15–0.20) of the trees with low DBH (i.e., regeneration and understory trees), while the dominant trees were accurately detected (R2 = 0.61). The stem biomass was accurately estimated, presenting an R2 of 0.67. Overall, despite some products’ low accuracy, their full and efficient exploitability within the aforementioned proposed methodology has been endeavored with the aim of actively contributing to long-term sustainable forest management.
Symbiosis Mechanism of Associated Bacteria on 2-keto-L-gulonic Acid Production via Mixed Fermentation: A Review
Authors: Chen Wenhu , Liu Qian , Liu Meng , Liu Hongling , Huang Di , Jiang Yi , Wang Tengfei , Yuan Haibo
Published: November 2023

Vitamin C, a water-soluble vitamin with strong reducing power, cannot be synthesized by the human body and participates in a variety of important biochemical reactions. Vitamin C is widely used in the pharmaceutical, food, health care, beverage, cosmetics, and feed industries, with a huge market demand. The classical two-step fermentation method is the mainstream technology for vitamin C production. D-sorbitol is transformed into L-sorbose by Gluconobacter oxydans in the first step of fermentation; then, L-sorbose is transformed into 2-keto-L-gulonic acid (2-KGA) by a coculture system composed of Ketogulonicigenium vulgare and associated bacteria; and finally, 2-KGA is transformed into vitamin C through chemical transformation. The conversion of L-sorbose into 2-KGA in the second fermentation step is performed by K. vulgare. However, considering the slow growth and low 2-KGA production of K. vulgare when cultured alone, it is necessary to add an associated bacteria to stimulate K. vulgare growth and 2-KGA production. Although the mechanism by which the associated bacteria promote K. vulgare growth and 2-KGA production has extensively been studied, this remains a hot topic in related fields. Based on the latest achievements and research, this review summarizes the metabolic characteristics of K. vulgare and associated bacteria and elucidates the mechanism by which the associated bacteria promote the growth and 2-KGA production of K. vulgare.
Optimization of Fermentation Conditions for Biocatalytic Conversion of Decanoic Acid to Trans-2-Decenoic Acid
Authors: Nie Shihao , Liu Keyi , Liu Ben , Li Piwu , Su Jing
Published: November 2023

Trans-2-decenoic acid has a wide range of applications, including those in medicine, food, and health care. Therefore, the industrial production of trans-2-decenoic acid is particularly important. However, few studies have focused on medium-chain unsaturated fatty acids. Therefore, we aimed to optimize the fermentation process of decanoic acid biocatalysis to synthesize trans-2-decenoic acid using an engineered Escherichia coli constructed in the laboratory. Early-stage culture and the effect of the seed liquid culture time, culture temperature, inoculum amount, induction temperature, dissolution effects of the substrate solvent, metal ions, and substrate loading on the titer of trans-2-decenoic acid were evaluated. Based on a single-factor experimental optimization, a Box–Behnken design (BBD) was used for response surface testing using the substrate feeding concentration, inducer concentration, and MnCl2 concentration as response variables and trans-2-decenoic acid production as the response value. The optimal fermentation process was as follows: Seed culture time of 20 h, culture temperature of 37 °C, inoculation amount of 1%, induction temperature of 30 °C, substrate flow of 0.15 g/L, inducer concentration of 5.60 g/L, and MnCl2 concentration of 0.10 mM. Under these conditions, the average production of trans-2-decenoic acid was 1.982 ± 0.110 g/L, which was 1.042 g/L higher than that obtained in the basic LB medium. Compared with that of the previous period, the titer of the trans-2-decenoic acid studied increased by 1.501 ± 0.110 g/L, providing a basis for further research on the fermentation process of the biocatalytic decanoic acid synthesis of trans-2-decenoic acid.
Thermal Tolerance of Spotted Sea Bass (Lateolabrax maculatus) and Pearl Gentian Grouper (Epinephelus fuscoguttatus Female × E. lanceolatus Male) in Different Environmental Temperatures
Authors: Lin Heshan , Huang Yaqin , Zhang Kun , Bo Jun , Zheng Ronghui , Ye Youling , Liu Kun
Published: November 2023

Seawater cooling is the most common way to cool down nuclear power plants. However, the thermal drainage of nuclear power plants results in sharp rises in local seawater temperatures and, therefore, affects fisheries and ecologies. Therefore, evaluating the thermal tolerance of marine organisms not only provides further insight into their biological characteristics but also holds significant importance for the site selection and construction of nuclear power plants. This study investigated the thermal tolerance of spotted sea bass (Lateolabrax maculatus) and pearl gentian grouper (Epinephelus fuscoguttatus female × E. lanceolatus male) using the critical thermal maximum method. The environmental temperatures for spotted sea bass and grouper in spring, summer, autumn, and winter were 21.6/23 °C, 26.5/25.9 °C, 25.0/25.9 °C, and 14.5/16.6 °C, respectively. Under four seasonal conditions, different temperature rise rates of +2 °C, +4 °C, +6 °C, and +8 °C per hour were set. The maximum critical temperature, initial lethal temperature, absolute lethal temperature, and semi-lethal temperature were recorded for both fish species. The results show that the rates of temperature increase did not affect the thermal tolerance of either fish species. In all seasons, the fish experienced rapid death once they reached the initial lethal temperature. However, there was a significant correlation of season with thermal tolerance in both fish species. For spotted sea bass, the semi-lethal temperature in summer reached about 40 °C, while in winter it was about 35 °C. For pearl gentian grouper, the semi-lethal temperature in summer reached about 40 °C, while in winter, it was about 38 °C. These results remind us that these two fish species have a limited ability to adapt to rapid temperature rises, but appropriate acclimation temperatures can effectively improve their thermal tolerance. Therefore, it is necessary to further consider the potential impacts on these fish species during the site selection and construction process of nuclear power plants.
Eco-Dynamic Analysis of the Community Structure of Nekton in the Northern South China Sea
Authors: Yuan Huarong , Lin Zhaojin , Chen Yuxiang , Chen Pimao
Published: November 2023

The universal laws of thermodynamics in the process of ecosystem development have long been the common research focus of ecology and biophysics. Eco-exergy from thermodynamics is a popular theory in the study of ecosystem self-organization that has been widely used in the study of wetlands and aquatic ecosystems. This study is based on the data of bottom trawl fishery resources in the Northern South China Sea in 1964–1965, 1997–1999, 2006–2007, and 2017. Based on the eco-exergy theory, the exergy contribution rate (PC) of the nekton community and the exergy contribution rate (PL) of different organismic populations were constructed. The eco-exergy (EX) and specific eco-exergy (EXsp) of the nekton in the northeastern South China Sea were analyzed. The results show that, from 1964 to 2017, the EX and EXsp of the nekton community decreased 13.28-fold and 1.42-fold, respectively. Fish populations remained the major contributors to the EX and EXsp of the nekton community; however, compared to crustaceans and cephalopods, their role in maintaining the stability and complexity of the community structure was gradually weakened, and the genetic information per unit of biomass decreased. Meanwhile, compared to fish, the proportion of the EX of crustaceans and cephalopods in the nekton community showed an upward trend. The proportion of crustaceans increased from 2.76% in 1997–1999 to 14.84% in 2017, while that of cephalopods increased from 3.55% to 16.67%. Based on the findings, we speculate that crustaceans and cephalopods play an increasing role in the stability and complexity of the fishery resource structure in the Northern South China Sea. The species replacement in the nekton was obvious, and the dominant species of the Nekton community gradually changed from k-type species to r-type species in the Northern South China Sea.
Reducing Scour around Semi-Elliptical Bridge Abutments: Application of Roughness Elements
Authors: Rezaie Afsaneh , Afzalimehr Hossein , Sohrabi Sina , Nazari-Sharabian Mohammad , Karakouzian Moses , Ahmadi Reza
Published: November 2023

Bridge abutments in river channels induce local scour. Recent research indicates that introducing roughness elements on the surface of the bridge abutments can influence the flow pattern around the abutment, reducing the intensity of eddies and diverting the flow away from the abutment. The roughness elements protruding from the abutment surface, with specific thickness, protrusion, and spacing, influence the scour process by enhancing turbulence. This study investigates the impact of roughness elements and their spacing on clear water scour at bridge abutments. The results reveal a noteworthy reduction in scour depth as the spacing between roughness elements decreases and their thickness increases on the abutment surface. Furthermore, an increase in the roughness spacing to roughness protrusion ratio (s/p) leads to an amplified scour depth. Additionally, the presence of roughness on the abutment surface alters the slope characteristics of the scour hole in response to changes in flow depth. In particular, the absence of roughness exhibits an increased slope as flow depth increases, while the presence of roughness results in a reduced slope across all three flow depths examined. Notably, the maximum slope and depth of the scour hole under the influence of roughness elements occurs at angles of 50 to 70 degrees. Also, the slope and depth of the scour hole decrease to a minimum value at specific roughness dimensions (s = 0.17 L and p = 0.17 L).
Post-Harvest Atmospheric Pressure and Composition Modify the Concentration and Bioaccessibility of α- and β-Carotene in Carrots and Sweet Potatoes
Authors: Hamieh Batoul , Borel Patrick , Raouche Sana , Bruzzese Laurie , Adjriou Nabil , Halimi Charlotte , Marconot Gregory , Gillet Guillian , Rostain Jean-Claude , Guieu Régis , Desmarchelier Charles
Published: November 2023

Provitamin A (proVA) carotenoid synthesis and degradation are strongly influenced by environmental factors, including during post-harvest storage. Hypobaric and hyperbaric storages increase the shelf-life of many crops, but their effects on proVA carotenoids are not known. Our aim was to investigate the effects of modifications of atmospheric pressure and composition on α- and β-carotene concentration and bioaccessibility during the post-harvest storage of carrots and sweet potatoes. Vegetables were stored for 11–14 days at 20 °C in the dark in chambers with modified pressure and O2 concentrations. In carrots, α- and β-carotene concentrations increased significantly during storage, but compared to the control, they were significantly lower in hyperbaria (−23 and −26%, respectively), whereas they did not differ significantly in hypoxia and hypobaria. In sweet potatoes, α- and β-carotene concentrations decreased significantly during storage, but neither hypoxia, hypobaria nor hyperbaria led to any significant change compared to the control. There was a significant increase for carrot α- and β-carotene bioaccessibility in hypobaria and hyperbaria, while there was a significant decrease for sweet potato β-carotene bioaccessibility in hypobaria/hypoxia and normobaria/hypoxia (−45% and −65% vs. control, respectively). Atmospheric pressure and composition during the post-harvest storage of carrots and sweet potatoes modified the concentration and bioaccessibility of proVA carotenoids.
Insects, Plants, and Microorganisms from Dry Lands as Novel Sources of Proteins and Peptides for Human Consumption
Authors: Ramírez-Guzmán Nathiely , Torres-León Cristian , Aguillón-Gutiérrez David , Aguirre-Joya Jorge Alejandro
Published: November 2023

Protein malnutrition is present in developing countries but also in developed ones due to actual eating habits involving insufficient protein intake. In addition to this, it is estimated by the Food and Agricultural Organization of the United Nations that the world’s population will increase to 9.1 billion people in less than 30 years. This poses a significant challenge in terms of nourishing the population. Different strategies have been proposed to address this challenge, including exploring novel protein sources such as plants. For instance, Prosopis alba pods have an 85.5% protein content. Other examples are microorganisms, such as Halobacillus adaensis which produces 571 U/mL of protease, and insects such as those belonging to the Orthoptera order, like grasshoppers, which have a protein content of 65.96%. These sources have been found in dry lands and are being explored to address this challenge.
An Analysis of the Effects of Lifestyle Changes by Using a Fractional-Order Population Model of the Overweight/Obese Diabetic Population
Authors: Albalawi Kholoud Saad , Malik Kuldeep , Alkahtani Badr Saad T. , Goswami Pranay
Published: November 2023

Unbalanced lifestyles and other underlying medical conditions are responsible for the worrying pace at which diabetes mellitus is becoming a global health crisis. Recent studies suggest that placing a diabetic patient into remission through a rigorous lifestyle change program can normalize blood glucose levels. This research focuses on fractional order derivative-based mathematical modeling and analysis of the diabetes mellitus model with remission parameters. Firstly, the existence and uniqueness of the solution of the diabetes mellitus model are discussed. Non-negativity and boundedness are also examined. Afterward, the concept of the Jacobian matrix is used to investigate the stability of the model’s equilibrium points. The Daftardar-Gejji and Jafari Method has finally been applied to approximate the solutions. The conclusions drawn from numerical simulations of the diabetic model with fractional-order derivatives show a clear dependence on the remission parameters and fractional-order derivative.
Miniaturization and Bandwidth Enhancement of Fractal-Structured Two-Arm Sinuous Antenna Using Gap Loading with Meandering
Authors: Kim Junghyeon , Keun Jongho , Yoo Taehoon , Lim Sungjoon
Published: November 2023

A sinuous antenna is a frequency-independent antenna known for its wide bandwidth and consistent gain, which makes it valuable in broadband applications such as ultrawideband (UWB) radar and ground-penetrating radar (GPR). However, sinuous antennas tend to be rather large. Consequently, numerous studies have explored miniaturization methods, with the gap-loading method emerging as a prominent approach. Unfortunately, it is still difficult to achieve broad bandwidths for conventional miniaturized sinuous antennas. In this paper, we use a novel approach incorporating a meander shape into the sinuous curve and employing gap loading with meandering. This innovative technique results in the development of a fractal-structured two-arm sinuous antenna characterized by an ultra-compact size and significantly expanded bandwidth. Adding a meander line in the outermost part maximizes the capacitance, thereby enhancing the gap-loading effect and minimizing the overall size of the sinuous antenna. In addition, the introduction of an inner meander line increases the inductance, contributing to a further expansion of the antenna’s bandwidth. For example, the electrical length of the antenna without the meander line is 0.552 × 0.552 × 0.052 λg3, while the electrical length of the antenna with the meander line is only 0.445 × 0.445 × 0.036 λg3, i.e., 19.4% smaller. The antenna lacking the outermost meander line exhibits a 10 dB impedance bandwidth, spanning from 0.74 to 10.53 GHz. In contrast, the antenna featuring the outermost meander line has a 10 dB impedance bandwidth, extending from 0.51 to 10.72 GHz, which results in a remarkable enhancement in the fractional bandwidth (by 8.1%). Hence, the proposed antenna design is a good candidate for broadband applications that require miniaturization.
Comparative Analysis of Algorithms to Cleanse Soil Micro-Relief Point Clouds
Authors: Ott Simone , Burkhard Benjamin , Harmening Corinna , Paffenholz Jens-André , Steinhoff-Knopp Bastian
Published: November 2023

Detecting changes in soil micro-relief in farmland helps to understand degradation processes like sheet erosion. Using the high-resolution technique of terrestrial laser scanning (TLS), we generated point clouds of three 2 × 3 m plots on a weekly basis from May to mid-June in 2022 on cultivated farmland in Germany. Three well-known applications for eliminating vegetation points in the generated point cloud were tested: Cloth Simulation Filter (CSF) as a filtering method, three variants of CANUPO as a machine learning method, and ArcGIS PointCNN as a deep learning method, a sub-category of machine learning using deep neural networks. We assessed the methods with hard criteria such as F1 score, balanced accuracy, height differences, and their standard deviations to the reference surface, resulting in data gaps and robustness, and with soft criteria such as time-saving capacity, accessibility, and user knowledge. All algorithms showed a low performance at the initial measurement epoch, increasing with later epochs. While most of the results demonstrate a better performance of ArcGIS PointCNN, this algorithm revealed an exceptionally low performance in plot 1, which is describable by the generalization gap. Although CANUPO variants created the highest amount of data gaps, we recommend that CANUPO include colour values in combination with CSF.
Kinesthetic Experience: Emancipatory Corporeal Scores
Authors: Vara Martín Celia
Published: November 2023

This article investigates the corporeal practices by the Catalonian artist Fina Miralles (b.1950) in some of her performances during the 1970s. I specifically focus my analysis on the manner in which the artist verifies the existence of her body under the acute political restrictions on the body during the last years of Francoism (1939–1975). I argue that she does this by a process of sensorial investigations, which include painting, filming and touching natural elements, and moving them and leaving different types of tracks, which lead to generating corporeal scores and body mapping. I elaborate on the way that producing corporeal sensorial knowledge generated from her body mapping and kinaesthetic knowledge is a transgressive and emancipatory feminist intervention. My argument is that kinesthesia generates a process of body-mapping awareness within the body and its movement, which reinforces a sensorial way of knowledge that leads to a reconstitution of the body that function as corporeal agency. Based on feminist theories of embodiment and agency, taking the Carrie Noland concept of kinesthesia (2009) as a central analytical tool, and with a background as a psychologist, I approach this research with embodied methodologies (conversations with the artist, recreation of her actions, etc.) and draw mainly from research-creation methods and kinaesthetic empathy.
Preliminary Insights into the Diagnostic Accuracy of the Modified Arm Care Screen Test for Overhead Athletes: An On-Field Tool for Injury Prevention
Authors: Paraskevopoulos Eleftherios , Kottaridis Fotis-Marios , Moutzouri Maria , Koumantakis George A. , Antonakis-Karamintzas Dimitrios , Tsolakis Charilaos , Koulouvaris Panagiotis , Christakou Anna , Papandreou Maria
Published: November 2023

Background: The Arm Care Screen was developed to detect musculoskeletal limitations that could affect performance or even increase the risk for shoulder injuries in overhead athletes. This study aimed to assess the discriminant validity of the modified Arm Care Screen in overhead athletes. Methods: Sixty-two overhead athletes (mean age: 24.5 ± 4.2 years) were recruited. The athletes underwent a comprehensive Arm Care Screen evaluation, including reciprocal shoulder mobility, total body rotation, lower body diagonal reach, and rotary stability assessments. Ten different musculoskeletal measurements were independently measured by two physical therapists. Results: The modified Arm Care Screen showed moderate to strong associations (phi values ranged from 0.273 to 0.905) with the respective musculoskeletal range of motion and balance measurements. Sensitivity ranged from 81.25% to 88.57%, indicating a high true positive rate, and specificity ranged from 43.75% to 94.44%, indicating a moderate to strong positive rate. Positive and negative likelihood ratios ranged from 1.48 to 15.92 and 0.12 to 0.38, respectively. The positive and negative predictive value ranged from 58.14% to 92% and from 73.68% to 93.18%, respectively. The accuracy of the modified ACS ranged from 62.90% to 91.94%. Conclusion: The modified Arm Care Screen demonstrated promising diagnostic accuracy in identifying significant movement restrictions.
The Plasma Membrane Purinoreceptor P2K1/DORN1 Is Essential in Stomatal Closure Evoked by Extracellular Diadenosine Tetraphosphate (Ap4A) in Arabidopsis thaliana
Authors: Dobrogojski Jędrzej , Nguyen Van Hai , Kowalska Joanna , Borek Sławomir , Pietrowska-Borek Małgorzata
Published: November 2023

Dinucleoside polyphosphates (NpnNs) are considered novel signalling molecules involved in the induction of plant defence mechanisms. However, NpnN signal recognition and transduction are still enigmatic. Therefore, the aim of our research was the identification of the NpnN receptor and signal transduction pathways evoked by these nucleotides. Earlier, we proved that purine and pyrimidine NpnNs differentially affect the phenylpropanoid pathway in Vitis vinifera suspension-cultured cells. Here, we report, for the first time, that both diadenosine tetraphosphate (Ap4A) and dicytidine tetraphosphate (Cp4C)-induced stomatal closure in Arabidopsis thaliana. Moreover, we showed that plasma membrane purinoreceptor P2K1/DORN1 (does not respond to nucleotide 1) is essential for Ap4A-induced stomata movements but not for Cp4C. Wild-type Col-0 and the dorn1-3 A. thaliana knockout mutant were used. Examination of the leaf epidermis dorn1-3 mutant provided evidence that P2K1/DORN1 is a part of the signal transduction pathway in stomatal closure evoked by extracellular Ap4A but not by Cp4C. Reactive oxygen species (ROS) are involved in signal transduction caused by Ap4A and Cp4C, leading to stomatal closure. Ap4A induced and Cp4C suppressed the transcriptional response in wild-type plants. Moreover, in dorn1-3 leaves, the effect of Ap4A on gene expression was impaired. The interaction between P2K1/DORN1 and Ap4A leads to changes in the transcription of signalling hubs in signal transduction pathways.
Dysfunction of Human Estrogen Signaling as a Novel Molecular Signature of Polycystic Ovary Syndrome
Authors: Marie Clémentine , Pierre Alice , Mayeur Anne , Giton Frank , Corre Raphael , Grynberg Michaël , Cohen-Tannoudji Joëlle , Guigon Céline J. , Chauvin Stéphanie
Published: November 2023

Estradiol (E2) is a major hormone-controlling folliculogenesis whose dysfunction may participate in polycystic ovary syndrome (PCOS) infertility. To determine whether both the concentration and action of E2 could be impaired in non-hyperandrogenic overweight PCOS women, we isolated granulosa cells (GCs) and follicular fluid (FF) from follicles of women undergoing ovarian stimulation (27 with PCOS, and 54 without PCOS). An analysis of the transcript abundance of 16 genes in GCs showed that androgen and progesterone receptor expressions were significantly increased in GCs of PCOS (by 2.7-fold and 1.5-fold, respectively), while those of the steroidogenic enzymes CYP11A1 and HSD3B2 were down-regulated (by 56% and 38%, respectively). Remarkably, treatment of GC cultures with E2 revealed its ineffectiveness in regulating the expression of several key endocrine genes (e.g., GREB1 or BCL2) in PCOS. Additionally, a comparison of the steroid concentrations (measured by GC/MS) in GCs with those in FF of matched follicles demonstrated that the significant decline in the E2 concentration (by 23%) in PCOS FF was not the result of the E2 biosynthesis reduction. Overall, our study provides novel hallmarks of PCOS by highlighting the ineffective E2 signaling in GCs as well as the dysregulation in the expression of genes involved in follicular growth, which may contribute to aberrant folliculogenesis in non-hyperandrogenic women with PCOS.
Transcriptome Dynamics of Brassica juncea Leaves in Response to Omnivorous Beet Armyworm (Spodoptera exigua, Hübner)
Authors: Xia Rui , Xu Liai , Hao Jiaojiao , Zhang Lili , Wang Shanyi , Zhu Zhujun , Yu Youjian
Published: November 2023

Cruciferous plants manufacture glucosinolates (GSLs) as special and important defense compounds against insects. However, how insect feeding induces glucosinolates in Brassica to mediate insect resistance, and how plants regulate the strength of anti-insect defense response during insect feeding, remains unclear. Here, mustard (Brassica juncea), a widely cultivated Brassica plant, and beet armyworm (Spodoptera exigua), an economically important polyphagous pest of many crops, were used to analyze the changes in GSLs and transcriptome of Brassica during insect feeding, thereby revealing the plant–insect interaction in Brassica plants. The results showed that the content of GSLs began to significantly increase after 48 h of herbivory by S. exigua, with sinigrin as the main component. Transcriptome analysis showed that a total of 8940 DEGs were identified in mustard challenged with beet armyworm larvae. The functional enrichment results revealed that the pathways related to the biosynthesis of glucosinolate and jasmonic acid were significantly enriched by upregulated DEGs, suggesting that mustard might provide a defense against herbivory by inducing JA biosynthesis and then promoting GSL accumulation. Surprisingly, genes regulating JA catabolism and inactivation were also activated, and both JA signaling repressors (JAZs and JAMs) and activators (MYCs and NACs) were upregulated during herbivory. Taken together, our results indicate that the accumulation of GSLs regulated by JA signaling, and the regulation of active and inactive JA compound conversion, as well as the activation of JA signaling repressors and activators, collectively control the anti-insect defense response and avoid over-stunted growth in mustard during insect feeding.
Donor Heart Preservation: Current Knowledge and the New Era of Machine Perfusion
Authors: Kounatidis Dimitris , Brozou Vassiliki , Anagnostopoulos Dimitris , Pantos Constantinos , Lourbopoulos Athanasios , Mourouzis Iordanis
Published: November 2023

Heart transplantation remains the conventional treatment in end-stage heart failure, with static cold storage (SCS) being the standard technique used for donor preservation. Nevertheless, prolonged cold ischemic storage is associated with the increased risk of early graft dysfunction attributed to residual ischemia, reperfusion, and rewarming damage. In addition, the demand for the use of marginal grafts requires the development of new methods for organ preservation and repair. In this review, we focus on current knowledge and novel methods of donor preservation in heart transplantation. Hypothermic or normothermic machine perfusion may be a promising novel method of donor preservation based on the administration of cardioprotective agents. Machine perfusion seems to be comparable to cold cardioplegia regarding donor preservation and allows potential repair treatments to be employed and the assessment of graft function before implantation. It is also a promising platform for using marginal organs and increasing donor pool. New pharmacological cardiac repair treatments, as well as cardioprotective interventions have emerged and could allow for the optimization of this modality, making it more practical and cost-effective for the real world of transplantation. Recently, the use of triiodothyronine during normothermic perfusion has shown a favorable profile on cardiac function and microvascular dysfunction, likely by suppressing pro-apoptotic signaling and increasing the expression of cardioprotective molecules.
Antifungal Effect of Bacillus velezensis ZN-S10 against Plant Pathogen Colletotrichum changpingense and Its Inhibition Mechanism
Authors: Ye Qingling , Zhong Zhupeiqi , Chao Shufeng , Liu Lu , Chen Mengli , Feng Xiaoxiao , Wu Huiming
Published: November 2023

In order to optimize crop production and mitigate the adverse impacts associated with the utilization of chemical agents, it is necessary to explore new biocontrol agents. Bacillus velezensis has been widely studied as a biocontrol agent because of its efficient and ecofriendly plant disease control mechanisms. This study shows that the strain ZN-S10 effectively reduces the area of leaf spots caused by the pathogen Colletotrichum changpingense ZAFU0163-1, which affects conidia production and germination, inhibits mycelium growth, and induces mycelium deformation. In antifungal experiments with crude extracts, we observed a delay in the cell cycle of conidia, which may be responsible for the inhibition of conidial germination. Among the bioactive metabolites detected through integrated LC-MS- and GC-MS-based untargeted metabolomics, 7-O-Succinyl macrolactin A, telocinobufagin, and surfactin A may be the main antifungal metabolites of strain ZN-S10. The presence of 7-O-Succinyl macrolactin A could explain the cell damage in germ tubes. This is the first report of telocinobufagin detected in B. velezensis. These results are significant for understanding the inhibitory mechanisms employed by B. velezensis and should serve as a reference in the production of biocontrol agents.
PML Body Biogenesis: A Delicate Balance of Interactions
Authors: Silonov Sergey A. , Smirnov Eugene Y. , Kuznetsova Irina M. , Turoverov Konstantin K. , Fonin Alexander V.
Published: November 2023

PML bodies are subnuclear protein complexes that play a crucial role in various physiological and pathological cellular processes. One of the general structural proteins of PML bodies is a member of the tripartite motif (TRIM) family—promyelocytic leukemia protein (PML). It is known that PML interacts with over a hundred partners, and the protein itself is represented by several major isoforms, differing in their variable and disordered C-terminal end due to alternative splicing. Despite nearly 30 years of research, the mechanisms underlying PML body formation and the role of PML proteins in this process remain largely unclear. In this review, we examine the literature and highlight recent progress in this field, with a particular focus on understanding the role of individual domains of the PML protein, its post-translational modifications, and polyvalent nonspecific interactions in the formation of PML bodies. Additionally, based on the available literature, we propose a new hypothetical model of PML body formation.
Attenuation of Vanadium-Induced Neurotoxicity in Rat Hippocampal Slices (In Vitro) and Mice (In Vivo) by ZA-II-05, a Novel NMDA-Receptor Antagonist
Authors: Ladagu Amany Digal , Olopade Funmilayo Eniola , Chazot Paul , Oyagbemi Ademola A. , Ohiomokhare Samuel , Folarin Oluwabusayo Racheal , Gilbert Taidinda Tashara , Fuller Madison , Luong Toan , Adejare Adeboye , Olopade James O.
Published: November 2023

Exposure to heavy metals, such as vanadium, poses an ongoing environmental and health threat, heightening the risk of neurodegenerative disorders. While several compounds have shown promise in mitigating vanadium toxicity, their efficacy is limited. Effective strategies involve targeting specific subunits of the NMDA receptor, a glutamate receptor linked to neurodegenerative conditions. The potential neuroprotective effects of ZA-II-05, an NMDA receptor antagonist, against vanadium-induced neurotoxicity were explored in this study. Organotypic rat hippocampal slices, and live mice, were used as models to comprehensively evaluate the compound’s impact. Targeted in vivo fluorescence analyses of the hippocampal slices using propidium iodide as a marker for cell death was utilized. The in vivo study involved five dams, each with eight pups, which were randomly assigned to five experimental groups (n = 8 pups). After administering treatments intraperitoneally over six months, various brain regions were assessed for neuropathologies using different immunohistochemical markers. High fluorescence intensity was observed in the hippocampal slices treated with vanadium, signifying cell death. Vanadium-exposed mice exhibited demyelination, microgliosis, and neuronal cell loss. Significantly, treatment with ZA-II-05 resulted in reduced cellular death in the rat hippocampal slices and preserved cellular integrity and morphological architecture in different anatomical regions, suggesting its potential in countering vanadium-induced neurotoxicity.
Identification and Analysis of Candidate Genes Associated with Maize Fusarium Cob Resistance Using Next-Generation Sequencing Technology
Authors: Sobiech Aleksandra , Tomkowiak Agnieszka , Bocianowski Jan , Szymańska Grażyna , Nowak Bartosz , Lenort Maciej
Published: November 2023

The pressure to reduce mineral fertilization and the amount of pesticides used has become a factor limiting production growth, as has the elimination of many crop protection chemicals from the market. A key condition for this to be an effective form of protection is the use of varieties with higher levels of resistance. The most effective and fastest way to assist in the selection and control of pathogens is the conducting of genome-wide association studies. These are useful tools for identifying candidate genes, especially when combined with QTL mapping to map and validate loci for quantitative traits. The aim of this study was to identify new markers coupled to genes that determine maize plant resistance to fusarium head blight through the use of next-generation sequencing, association and physical mapping, and to optimize diagnostic procedures to identify selected molecular markers coupled to plant resistance to this fungal disease. As a result of field experiments and molecular analyses, molecular markers coupled to potential genes for resistance to maize ear fusariosis were selected. The newly selected markers were tested against reference genotypes. As a result of the analyses, it was found that two markers (11801 and 20607) out of the ten that were tested differentiated between susceptible and resistant genotypes. Marker number 11801 proved to be the most effective, with a specious product of 237 bp appearing for genotypes 1, 3, 5, 9 and 10. These genotypes were characterized by a field resistance of 4–6 on the 9° scale (1 being susceptible, 9 being resistant) and for all genotypes except 16 and 20, which were characterized by a field resistance of 9. In the next step, this marker will be tested on a wider population of extreme genotypes in order to use it for the preliminary selection of fusarium-resistant genotypes, and the phosphoenolpyruvate carboxylase kinase 1 gene coupled to it will be subjected to expression analysis.
Recombinant Laminin-511 Fragment (iMatrix-511) Coating Supports Maintenance of Human Nucleus Pulposus Progenitor Cells In Vitro
Authors: Soma Hazuki , Sakai Daisuke , Nakamura Yoshihiko , Tamagawa Shota , Warita Takayuki , Schol Jordy , Matsushita Erika , Naiki Mitsuru , Sato Masato , Watanabe Masahiko
Published: November 2023

The angiopoietin-1 receptor (Tie2) marks specific nucleus pulposus (NP) progenitor cells, shows a rapid decline during aging and intervertebral disc degeneration, and has thus sparked interest in its utilization as a regenerative agent against disc degeneration. However, the challenge of maintaining and expanding these progenitor cells in vitro has been a significant hurdle. In this study, we investigated the potential of laminin-511 to sustain Tie2+ NP progenitor cells in vitro. We isolated cells from human NP tissue (n = 5) and cultured them for 6 days on either standard (Non-coat) or iMatrix-511 (laminin-511 product)-coated (Lami-coat) dishes. We assessed these cells for their proliferative capacity, activation of Erk1/2 and Akt pathways, as well as the expression of cell surface markers such as Tie2, GD2, and CD24. To gauge their regenerative potential, we examined their extracellular matrix (ECM) production capacity (intracellular type II collagen (Col2) and proteoglycans (PG)) and their ability to form spherical colonies within methylcellulose hydrogels. Lami-coat significantly enhanced cell proliferation rates and increased Tie2 expression, resulting in a 7.9-fold increase in Tie2-expressing cell yields. Moreover, the overall proportion of cells positive for Tie2 also increased 2.7-fold. Notably, the Col2 positivity rate was significantly higher on laminin-coated plates (Non-coat: 10.24% (±1.7%) versus Lami-coat: 26.2% (±7.5%), p = 0.010), and the ability to form spherical colonies also showed a significant improvement (Non-coat: 40.7 (±8.8)/1000 cells versus Lami-coat: 70.53 (±18.0)/1000 cells, p = 0.016). These findings demonstrate that Lami-coat enhances the potential of NP cells, as indicated by improved colony formation and proliferative characteristics. This highlights the potential of laminin-coating in maintaining the NP progenitor cell phenotype in culture, thereby supporting their translation into prospective clinical cell-transplantation products.
Wireframe DNA Origami for the Cellular Delivery of Platinum(II)-Based Drugs
Authors: De Luca Erik , Wang Yang , Baars Igor , De Castro Federica , Lolaico Marco , Migoni Danilo , Ducani Cosimo , Benedetti Michele , Högberg Björn , Fanizzi Francesco Paolo
Published: November 2023

The DNA origami method has revolutionized the field of DNA nanotechnology since its introduction. These nanostructures, with their customizable shape and size, addressability, nontoxicity, and capacity to carry bioactive molecules, are promising vehicles for therapeutic delivery. Different approaches have been developed for manipulating and folding DNA origami, resulting in compact lattice-based and wireframe designs. Platinum-based complexes, such as cisplatin and phenanthriplatin, have gained attention for their potential in cancer and antiviral treatments. Phenanthriplatin, in particular, has shown significant antitumor properties by binding to DNA at a single site and inhibiting transcription. The present work aims to study wireframe DNA origami nanostructures as possible carriers for platinum compounds in cancer therapy, employing both cisplatin and phenanthriplatin as model compounds. This research explores the assembly, platinum loading capacity, stability, and modulation of cytotoxicity in cancer cell lines. The findings indicate that nanomolar quantities of the ball-like origami nanostructure, obtained in the presence of phenanthriplatin and therefore loaded with that specific drug, reduced cell viability in MCF-7 (cisplatin-resistant breast adenocarcinoma cell line) to 33%, while being ineffective on the other tested cancer cell lines. The overall results provide valuable insights into using wireframe DNA origami as a highly stable possible carrier of Pt species for very long time-release purposes.
Predictive Factors and ACE-2 Gene Polymorphisms in Susceptibility to Long COVID-19 Syndrome
Authors: Varillas-Delgado David , Jimenez-Antona Carmen , Lizcano-Alvarez Angel , Cano-de-la-Cuerda Roberto , Molero-Sanchez Alberto , Laguarta-Val Sofia
Published: November 2023

Long COVID-19 syndrome is present in 5–10% of patients infected with SARS-CoV-2, and there is still little information on the predisposing factors that lead to its development. The purpose of the study was to evaluate the predictive factors in early symptoms, clinical features and the role of Angiotensin-Converting Enzyme-2 (ACE-2) c.513-1451G>A (rs2106806) and c.15643279T>C (rs6629110) polymorphisms in the susceptibility to developing Long COVID-19 syndrome subsequent to COVID-19 infectionA total of 29 patients who suffered COVID-19 were recruited in a descriptive longitudinal study of two groups: Long COVID-19 (n = 16) and non-Long COVID-19 (n = 13). Early symptoms and clinical features during COVID-19 were classified by a medical service. ACE-2 polymorphisms were genotyped by using a Single Nucleotide Primer Extension (SNPE). Of the early symptoms, fatigue, myalgia and headache showed a high risk of increasing Long COVID-19 susceptibility. Clinical features such as emergency care, SARS-CoV-2 reinfection, previous diseases, respiratory disease and brain fog also had a high risk of increasing Long COVID-19 susceptibility. The A allele in the rs2106806 variant was associated with an odds ratio (OR) of 4.214 (95% CI 2.521–8.853; p < 0.001), and the T allele in the rs6629110 variant was associated with an OR of 3.754 (95% CI 1.785–6.105; p = 0.002) of increasing Long COVID-19 susceptibility. This study shows the risk of ACE-2 polymorphisms, different early symptoms and clinical features during SARS-CoV-2 infection in susceptibility to Long COVID-19.
Folate-Appended Hydroxypropyl-β-Cyclodextrin Induces Autophagic Cell Death in Acute Myeloid Leukemia Cells
Authors: Kubota Yasushi , Hoshiko Toshimi , Higashi Taishi , Motoyama Keiichi , Okada Seiji , Kimura Shinya
Published: November 2023

Acute myeloid leukemia (AML) is a heterogenous myeloid neoplasm that remains challenging to treat. Because intensive conventional chemotherapy reduces survival rates in elderly patients, drugs with lower toxicity and fewer side effects are needed urgently. 2-Hydroxypropyl-β-cyclodextrin (HP-β-CyD) is used clinically as a pharmaceutical excipient for poorly water-soluble drugs. Previously, we showed that HP-β-CyD exerts antitumor activity by disrupting cholesterol homeostasis. Recently, we developed folate-conjugated HP-β-CyD (FA-HP-β-CyD) and demonstrated its potential as a new antitumor agent that induces not only apoptosis, but also autophagic cell death; however, we do not know whether FA-HP-β-CyD exerts these effects against AML. Here, we investigated the effects of FA-HP-β-CyD on folate receptor (FR)-expressing AML cells. We found that the cytotoxic activity of FA-HP-β-CyD against AML cells was stronger than that of HP-β-CyD. Also, FA-HP-CyD induced the formation of autophagosomes in AML cell lines. FA-HP-β-CyD increased the inhibitory effects of cytarabine and a BCL-2-selective inhibitor, Venetoclax, which are commonly used treat elderly AML patients. Notably, FA-HP-β-CyD suppressed the proliferation of AML cells in BALB/c nude recombinase-activating gene-2 (Rag-2)/Janus kinase 3 (Jak3) double-deficient mice with AML. These results suggest that FA-HP-β-CyD acts as a potent anticancer agent for AML chemotherapy by regulating autophagy.
Mitochondrial Cation Signalling in the Control of Inflammatory Processes
Authors: Pain Pampa , Spinelli Francesca , Gherardi Gaia
Published: November 2023

Mitochondria are the bioenergetic organelles responsible for the maintenance of cellular homeostasis and have also been found to be associated with inflammation. They are necessary to induce and maintain innate and adaptive immune cell responses, acting as signalling platforms and mediators in effector responses. These organelles are also known to play a pivotal role in cation homeostasis as well, which regulates the inflammatory responses through the modulation of these cation channels. In particular, this review focuses on mitochondrial Ca2+ and K+ fluxes in the regulation of inflammatory response. Nevertheless, this review aims to understand the interplay of these inflammation inducers and pathophysiological conditions. In detail, we discuss some examples of chronic inflammation such as lung, bowel, and metabolic inflammatory diseases caused by a persistent activation of the innate immune response due to a dysregulation of mitochondrial cation homeostasis.
Transcriptional Landscape of Repetitive Elements in Psoriatic Skin from Large Cohort Studies: Relevance to Psoriasis Pathophysiology
Authors: Krishnan Vidya S. , Kõks Sulev
Published: November 2023

While studies demonstrating the expression of repetitive elements (REs) in psoriatic skin using RNA-seq have been published before, not many studies have focused on the genome-wide expression patterns using larger cohorts. This study investigated the transcriptional landscape of differentially expressed REs in lesional and non-lesional skin from two previously published large datasets. We observed significant differential expression of REs in lesional psoriatic skin as well as the skin of healthy controls. Significant downregulation of several ERVs, HERVs (including HERV-K) and LINEs was observed in lesional psoriatic skin from both datasets. The upregulation of a small subset of HERV-Ks and Alus in lesional psoriatic skin was also reported. An interesting finding from this expression data was the significant upregulation and overlapping of tRNA repetitive elements in lesional and non-lesional psoriatic skin. The data from this study indicate the potential role of REs in the immunopathogenesis of psoriasis. The expression data from the two independent large study cohorts are powerful enough to confidently verify the differential expression of REs in relation to psoriatic skin pathology. Further studies are warranted to understand the functional impact of these repetitive elements in psoriasis pathogenesis, thereby expanding their significance as a potential targeting pathway for the disease treatment of psoriasis and other inflammatory diseases.