bluuebunny/crossref_metadata_2025 huggingface dataset:
Strong k-Skew Commutativity Preserving Maps on Standard Operator Algebras
Authors: Zhang Ting , Qi Xiaofei
Published: January 2025

Let A be a self-adjoint standard operator algebra on a real or complex Hilbert space of dimension ≥2, and let k∈{1,2,3}. The k-skew commutator for A,B∈A is defined by [A,B]1∗=AB−BA∗ and [A,B]k∗=[A,[A,[A,B]k−1∗1]1∗. Assume that Φ:A→A is a map whose range contains all rank-one projections. In this paper, we prove that Φ is strong k-skew-commutativity preserving, that is, [Φ(A),Φ(B)]k∗=[A,B]k∗ for all A,B∈A if and only if one of the following statements holds: (i) Φ is either the identity map or the negative identity map whenever k∈{1,3}; (ii) Φ is the identity map whenever k=2.
Editorial: Biomechanics, Health, Disease and Rehabilitation—2nd Edition
Authors: Taiar Redha
Published: January 2025

In the original article [...]
Perception and Longevity Control in Invertebrate Model Organisms—A Mini-Review of Recent Advances
Authors: Pontillo Nicholas , Lyu Yang
Published: January 2025

Perception alone can, in some cases, be sufficient to modulate aging and longevity. These influences on aging are perhaps mediated by changes in motivational states that regulate metabolism and physiology to impact health. Simple invertebrate models uniquely enable detailed dissection of integrative pathways linking perceptions to aging and remain the leading systems for advancing this field. Over the past 25 years, studies using the fruit fly Drosophila melanogaster and the nematode Caenorhabditis elegans have demonstrated that sensory cues, such as those related to food or mating, can influence aging independently of the physical acts associated with them. In this review, we highlight recent advancements in these invertebrate models, focusing on two key areas of progress: (i) the discovery of lifespan modulation driven by novel sensory cues across multiple modalities, including non-sexual social experience, light, and dietary choices; and (ii) the assignment of new aging-regulation functions to specific neurons downstream of sensory perception. The latter offers an exciting first glimpse at the neuronal circuits integrating sensory cues, motivational states, physiology, and aging.
Bioinspired Design and Applications of Liquid Gating Gas Valve Membranes
Authors: Li Yiyao , Liu Yang , Xu Rui , Liu Jing , Hou Xu
Published: January 2025

In nature, dynamic liquid interfaces play a vital role in regulating gas transport, as exemplified by the adaptive mechanisms of plant stomata and the liquid-lined alveoli, which enable efficient gas exchange through reversible opening and closing. These biological processes provide profound insights into the design of advanced gas control technologies. Inspired by these natural systems, liquid gating membranes have been developed utilizing capillary-stabilized liquids to achieve precise fluid regulation. These membranes offer unique advantages of rapid responses, stain resistance, and high energy efficiency. Particularly, they break through the limitations of traditional solid, porous membranes in gas transport. This perspective introduces bioinspired liquid gating gas valve membranes (LGVMs), emphasizing their opening/closing mechanism. It highlights how external stimuli can be exploited to enable advanced, multi-level gas control through active or passive regulation strategies. Diverse applications in gas flow regulation and selective gas transport are discussed. While challenges related to precise controllability, long-term stability, and scalable production persist, these advancements unlock significant opportunities for groundbreaking innovations across diverse fields, including gas purification, microfluidics, medical diagnostics, and energy harvesting technologies.
Sensors and Devices Based on Electrochemical Skin Conductance and Bioimpedance Measurements for the Screening of Diabetic Foot Syndrome: Review and Meta-Analysis
Authors: Verdini Federica , Mengarelli Alessandro , Chemello Gaetano , Salvatori Benedetta , Morettini Micaela , Göbl Christian , Tura Andrea
Published: January 2025

Diabetic foot syndrome is a multifactorial disease involving different etiological factors. This syndrome is also insidious, due to frequent lack of early symptoms, and its prevalence has increased in recent years. This justifies the remarkable attention being paid to the syndrome, although the problem of effective early screening for this syndrome, possibly at a patient’s home, is still unsolved. However, some options appear available in this context. First, it was demonstrated that the temperature measurement of the foot skin is an interesting approach, but it also has some limitations, and hence a more effective approach should combine data from temperature and from other sensors. For this purpose, foot skin conductance or bioimpedance measurement may be a good option. Therefore, the aim of this study was to review those studies where skin conductance/bioimpedance measurement was used for the detection of diabetic foot syndrome. In addition, we performed a meta-analysis of some of those studies, where a widely used device was exploited (SUDOSCAN®) for foot skin conductance measurement, and we found that skin conductance levels can clearly distinguish between groups of patients with and without diabetic neuropathy, the latter being one of the most relevant factors in diabetic foot syndrome.
Minocycline Ameliorates Staphylococcus aureus-Induced Neuroinflammation and Anxiety-like Behaviors by Regulating the TLR2 and STAT3 Pathways in Microglia
Authors: Zou Jiao , Gao Junwei , Shang Weilong , Fan Xiaotang
Published: January 2025

Background: Anxiety disorders are the most common mental illnesses. S. aureus is a Gram-positive opportunistic pathogen most commonly associated with anxiety-like behaviors. Minocycline ameliorates Gram-negative bacterial LPS-induced anxiety-like behaviors by suppressing microglia activation. However, the effects of minocycline on anxiety-like behaviors caused by S. aureus infections have received little attention. In this study, we aimed to investigate the molecular mechanism and effect of minocycline on anxiety-like behaviors caused by S. aureus infection. Methods: BV2 and N9 microglial cells were treated in vitro. The effects of minocycline on lipoteichoic acid (LTA)-stimulated inflammatory responses, STAT3 activation, and GLS1 expression were assessed using Western blotting, and cytokine secretion was determined using an ELISA. A mouse model was used to evaluate the capacity of minocycline to ameliorate anxiety-like behaviors caused by S. aureus infection. Results: We found that ≥100 μmol/L of minocycline remarkably attenuated LTA-induced TLR2 signaling pathway activation and proinflammatory cytokine expression in microglial cells. Minocycline prevented LTA-stimulated STAT3 activation and GLS1 expression in vitro. LTA-induced TLR2, TNF-α, IL-6, and GLS1 expression was markedly reduced by the inhibition of STAT3 phosphorylation. Mice were pretreated with 50 mg/kg of minocycline, significantly attenuating microglial activation and neuroinflammation. Minocycline also effectively alleviated the anxiety-like behaviors induced by S. aureus infection. Conclusions: Our findings indicate that minocycline alleviates S. aureus infection-induced anxiety-like behaviors by suppressing microglia activation.
MSEI-ENet: A Multi-Scale EEG-Inception Integrated Encoder Network for Motor Imagery EEG Decoding
Authors: Wu Pengcheng , Fei Keling , Chen Baohong , Pan Lizheng
Published: January 2025

Background: Due to complex signal characteristics and distinct individual differences, the decoding of a motor imagery electroencephalogram (MI-EEG) is limited by the unsatisfactory performance of suboptimal traditional models. Methods: A subject-independent model named MSEI-ENet is proposed for multiple-task MI-EEG decoding. It employs a specially designed multi-scale structure EEG-inception module (MSEI) for comprehensive feature learning. The encoder module further helps to detect discriminative information by its multi-head self-attention layer with a larger receptive field, which enhances feature representation and improves recognition efficacy. Results: The experimental results on Competition IV dataset 2a showed that our proposed model yielded an overall accuracy of 94.30%, MF1 score of 94.31%, and Kappa of 0.92. Conclusions: A performance comparison with state-of-the-art methods demonstrated the effectiveness and generalizability of the proposed model on challenging multi-task MI-EEG decoding.
Service Accessibility and Wellbeing in Amman’s Neighborhoods: A Comparative Study of Abdoun Al-Janoubi and Al-Zahra
Authors: Al-Homoud Majd , Aldahody Reema
Published: January 2025

Amman, the capital of Jordan, has undergone significant urbanization over the past seventy years, evolving from a small village into the nation’s largest city. This rapid growth has led to unpredictable population increases, creating socioeconomic disparities and affecting residents’ access to services and overall wellbeing. This study investigates the impact of service accessibility on wellbeing in two neighborhoods of Amman: Abdoun Al-Janoubi and Al-Zahra. Data were gathered from 492 household heads through structured interviews and systematic sampling. The study analyzed accessibility factors such as safety, convenience, comfort, and esthetics, and their influence on residents’ wellbeing, defined by positive emotions, social relationships, and overall life meaning. The results indicate a robust correlation between service accessibility and resident wellbeing, with Abdoun Al-Janoubi demonstrating superior accessibility and higher wellbeing compared to Al-Zahra. Key insights are illustrated, with data supporting the idea that enhanced urban service access improves quality of life. Safety and esthetics are critical factors, while comfort is less significant. Abdoun Al-Janoubi’s favorable attributes lead to higher wellbeing scores. Recommendations for urban planners include improving Al-Zahra’s infrastructure, enhancing esthetics, and optimizing public transport. Community organizations should promote social engagement, while local governments need to adjust zoning laws and upgrade public amenities.
Robust Cluster Prediction Across Data Types Validates Association of Sex and Therapy Response in GBM
Authors: Gibbs David L. , Cioffi Gino , Aguilar Boris , Waite Kristin A. , Pan Edward , Mandel Jacob , Umemura Yoshie , Luo Jingqin , Rubin Joshua B. , Pot David , Barnholtz-Sloan Jill
Published: January 2025

Background: Previous studies have described sex-specific patient subtyping in glioblastoma. The cluster labels associated with these “legacy data” were used to train a predictive model capable of recapitulating this clustering in contemporary contexts. Methods: We used robust ensemble machine learning to train a model using gene microarray data to perform multi-platform predictions including RNA-seq and potentially scRNA-seq. Results: The engineered feature set was composed of many previously reported genes that are associated with patient prognosis. Interestingly, these well-known genes formed a predictive signature only for female patients, and the application of the predictive signature to male patients produced unexpected results. Conclusions: This work demonstrates how annotated “legacy data” can be used to build robust predictive models capable of multi-target predictions across multiple platforms.
KEAP1-NRF2 Interaction in Cancer: Competitive Interactors and Their Role in Carcinogenesis
Authors: Oskomić Marina , Tomić Antonija , Barbarić Lea , Matić Antonia , Kindl Domagoj Christian , Matovina Mihaela
Published: January 2025

An American Cancer Society report estimates the emergence of around 2 million new cancer cases in the US in 2024 [...]
A Comparison of Established Diagnostic Criteria for Cachexia and Their Impacts on Prognostication in Patients with Oesophagogastric Cancer
Authors: Brown Leo R. , Soupashi Maria , Yule Michael S. , Grossart Cathleen M. , McMillan Donald C. , Laird Barry J. A. , Wigmore Stephen J. , Skipworth Richard J. E.
Published: January 2025

Background: Cachexia is common in patients with oesophagogastric cancer. The syndrome is characterised by tissue wasting (muscle and fat), anorexia, and reduced physical function, which result from complex interactions between the tumour and its host. Heterogeneity in the diagnostic criteria used for cachexia has hindered their clinical utilisation. This study aimed to compare the two established cachexia definitions (Fearon’s consensus definition and the Global Leadership Initiative on Malnutrition [GLIM] criteria) and their relationships with survival in patients with oesophagogastric cancer. Methods: Consecutive patients newly diagnosed with oesophagogastric cancer (January 2019 to December 2020) were identified from a prospective regional database. Involuntary weight loss, BMI, CT body composition analyses, and neutrophil–lymphocyte ratios were recorded at clinical staging. These data were used to assess patients for cachexia according to Fearon and GLIM diagnostic criteria. The primary outcome of interest was overall survival. Results: Overall, 465 patients (66.9% male, median 71 years) were diagnosed with oesophagogastric cancer during the 2-year study period. Cachectic proportions differed between definitions (Fearon: 59.1% vs. GLIM: 44.1%), and only 49.1% of the 322 patients who met one set of diagnostic criteria were cachectic according to both. Patients who met the GLIM criteria were significantly more comorbid and had a poorer performance status; however, no such difference was evident when using the Fearon definition. Those patients who met either set of diagnostic criteria had shorter survival than those who met neither (p < 0.001). Following adjustment for confounders, GLIM-defined cachexia was more strongly associated with reduced survival (aHR: 1.57 [95% CI: 1.25–1.96], p < 0.001) than Fearon-defined cachexia (aHR: 1.41 [95% CI: 1.13–1.76], p = 0.002). Patients who only met the Fearon diagnostic criteria had prolonged survival (median: 363 days) when compared to those who met only GLIM (median: 158 days) or both definitions (median: 120 days). A secondary analysis of those patients who met the GLIM diagnostic criteria (n = 205) compared the three potential phenotypical criteria used in this definition. Only reduced muscle mass, and not low BMI or weight loss, was associated with poorer survival (aHR: 1.88 [95% CI: 1.15–3.07], p = 0.012) in this group. Conclusions: Cancer cachexia is strongly associated with shortened survival in patients with oesophagogastric cancer. Classification using the GLIM criteria provides more effective prognostication and this definition should be utilised in multidisciplinary patient care.
A Deep Learning Approach for the Classification of Fibroglandular Breast Density in Histology Images of Human Breast Tissue
Authors: Heydarlou Hanieh , Hodson Leigh J. , Dorraki Mohsen , Hickey Theresa E. , Tilley Wayne D. , Smith Eric , Ingman Wendy V. , Farajpour Ali
Published: January 2025

Background: To progress research into the biological mechanisms that link mammographic breast density to breast cancer risk, fibroglandular breast density can be used as a surrogate measure. This study aimed to develop a computational tool to classify fibroglandular breast density in hematoxylin and eosin (H&E)-stained breast tissue sections using deep learning approaches that would assist future mammographic density research. Methods: Four different architectural configurations of transferred MobileNet-v2 convolutional neural networks (CNNs) and four different models of vision transformers were developed and trained on a database of H&E-stained normal human breast tissue sections (965 tissue blocks from 93 patients) that had been manually classified into one of five fibroglandular density classes, with class 1 being very low fibroglandular density and class 5 being very high fibroglandular density. Results: The MobileNet-Arc 1 and ViT model 1 achieved the highest overall F1 scores of 0.93 and 0.94, respectively. Both models exhibited the lowest false positive rate and highest true positive rate in class 5, while the most challenging classification was class 3, where images from classes 2 and 4 were mistakenly classified as class 3. The area under the curves (AUCs) for all classes were higher than 0.98. Conclusions: Both the ViT and MobileNet models showed promising performance in the accurate classification of H&E-stained tissue sections across all five fibroglandular density classes, providing a rapid and easy-to-use computational tool for breast density analysis.
Chronic IL-1-Exposed LNCaP Cells Evolve High Basal p62-KEAP1 Complex Accumulation and NRF2/KEAP1-Dependent and -Independent Hypersensitive Nutrient Deprivation Response
Authors: Dahl-Wilkie Haley , Gomez Jessica , Kelley Anastasia , Manjit Kirti , Mansoor Basir , Kanumuri Preethi , Pardo Sammy , Molleur Dana , Falah Rafah , Konakalla Anisha R. , Omiyale Morolake , Weintraub Susan , Delk Nikki A.
Published: January 2025

Chronic inflammation is a cancer hallmark and chronic exposure to interleukin-1 (IL-1) transforms castration-sensitive prostate cancer (PCa) cells into more fit castration-insensitive PCa cells. p62 is a scaffold protein that protects cells from nutrient deprivation via autophagy and from cytotoxic reactive oxygen via NFκB and NRF2 antioxidant signaling. Herein, we report that the LNCaP PCa cell line acquires high basal accumulation of the p62-KEAP1 complex when chronically exposed to IL-1. p62 promotes non-canonical NRF2 antioxidant signaling by binding and sequestering KEAP1 to the autophagosome for degradation. But despite high basal p62-KEAP1 accumulation, only two of several NRF2-induced genes analyzed, GCLC and HMOX1, showed high basal mRNA levels, suggesting that the high basal p62-KEAP1 accumulation does not result in overall high basal NRF2 activity. Nutrient starvation induces NRF2-dependent GCLC upregulation and HMOX1 repression, and we found that chronic IL-1-exposed LNCaP cells show hypersensitivity to serum starvation-induced GCLC and HMOX1 regulation. Thus, chronic IL-1 exposure affects cell response to nutrient stress. While HMOX1 expression remains NRF2/KEAP1-dependent in chronic IL-1-exposed LNCaP cells, GCLC expression is NRF2/KEAP1-independent. Furthermore, the high basal p62-KEAP1 complex accumulation is not required to regulate GCLC or HMOX1 expression, suggesting cells chronically exposed to IL-1 evolve a novel NRF2-independent role for the p62/KEAP1 axis.
Microvesicles Released by Osteoclastic Cells Exhibited Chondrogenic, Osteogenic, and Anti-Inflammatory Activities: An Evaluation of the Feasibility of Their Use for Treatment of Osteoarthritis in a Mouse Model
Authors: Sheng Matilda H.-C. , Rundle Charles H. , Lau Kin-Hing William
Published: January 2025

Extracellular vesicles (EVs), particularly exosomes (EXOs) of various skeletal and stem cells, were shown to delay osteoarthritis (OA) progression, and apoptotic bodies (ABs), another EV subtype, of osteoclasts showed osteoanabolic actions and were involved in the osteoclastic-regulation of local bone formation. Moreover, this study demonstrates that microvesicles (MVs) released by osteoclasts displayed potent pro-chondrogenic, pro-osteogenic, and anti-inflammatory activities. These activities were unique to osteoclastic MVs and were not shared by osteoclastic ABs and EXOs or MVs of other cell types. Because chronic synovial inflammation, progressive articular cartilage erosion, abnormal subchondral bone remodeling, and inability to regenerate articular cartilage are key etiologies of OA, we postulate that the foregoing activities of osteoclastic MVs could simultaneously target multiple etiologies of OA and could thereby be an effective therapy for OA. Accordingly, this study sought to assess the feasibility of an osteoclastic MV-based strategy for OA with a mouse tibial plateau injury model of OA. Briefly, tibial plateau injuries were created on the right knees of adult C57BL/6J mice, MVs were intraarticularly injected into the injured joints biweekly, and the OA progression was monitored histologically at five weeks post-injury. The MV treatment reduced the OA-induced losses of articular cartilage area and thickness, decreased irregularity in the articular cartilage surface, reduced loss of gliding/intermediate zone of articular cartilage, reduced osteophyte formation, suppressed synovial inflammation, and decreased the OARSI OA score. In summary, treatment with osteoclastic MVs delayed or reversed OA progression. Thus, this study supports the feasibility of an osteoclastic MV-based therapy for OA.
Heme-Oxygenase 1 Mediated Activation of Cyp3A11 Protects Against Non-Steroidal Pain Analgesics Induced Acute Liver Damage in Sickle Cell Disease Mice
Authors: Vats Ravi , Ungalara Ramakrishna , Dubey Rikesh K. , Sundd Prithu , Pradhan-Sundd Tirthadipa
Published: January 2025

Pain constitutes a significant comorbidity associated with sickle cell disease (SCD). Analgesics serve as the primary method for pain management; however, the long-term effects of these drugs on the liver of SCD patients remain not completely understood. Using real-time intravital imaging, we analyzed the effect of non-steroidal analgesics (NSA) in the liver of control and SS (SCD) mice. Remarkably, we found completely opposing effects in the liver of control and SS mice post-NSA treatment. Whereas SS mice were able to better tolerate the NSA treatment acutely compared to their littermate controls, in the long term, these mice showed delayed resolution of liver injury and exacerbated fibrosis compared to control mice. Mechanistically, we found that SS mice were protected from cytotoxicity caused by NSA at baseline due to the significant activation of hepatic Kupffer cells, which produced heme-oxygenase 1 (HO-1). HO-1 promoted the activation of the cytoprotective enzyme Cyp3A11, which inhibited hepatic damage caused by NSA. However, in the long term, depletion of hepatic Kupffer cells led to reduced expression of HO-1, which blocked the activation of Cyp3A11, resulting in fibrosis and a delay in the resolution of liver injury and inflammation. These preclinical data provide a strong proof-of-concept for HO-1 as well as Cyp3A11 as cytoprotectors against NSA-induced liver damage in the Townes model of SCD and support further development of these compounds as potential novel therapies for end-organ damage in SCD.
Lychee Peel Extract Ameliorates Hyperuricemia by Regulating Uric Acid Production and Excretion in Mice
Authors: Guo Zhenwang , Zhang Li , Liu Jinlei , Yang Ziming
Published: January 2025

Lychee peel generated during the industrial processing of lychee fruit are currently disposed of as agricultural waste. This study investigates the primary components of lychee peel extract (LPE) and the regulatory mechanisms of LPE on reducing uric acid (UA). Mice were injected with hypoxanthine and potassium oxonate to induce hyperuricemia and concurrently orally administered LPE. The analysis of the LPE composition reveals a predominance of polyphenolic compounds, including (-)-epicatechin, (-)-epigallocatechin, and procyanidin A2. In vitro tests have demonstrated that the LPE significantly inhibits the activity of xanthine oxidase (XOD). In vivo studies showed that LPE can reduce UA levels in hyperuricemia mice. Further mechanistic insights indicate that LPE inhibits hepatic XOD activity, thereby reducing UA synthesis within the organism. It also decreases the protein expression of urate transporter 1 (URAT1) and glucose transporter 9 (GLUT9), which leads to diminished UA reabsorption and increased excretion of UA. Additionally, LPE enhances the activity of superoxide dismutase (SOD) while simultaneously reducing malondialdehyde (MDA) contents, thereby improving antioxidant capacity in mice. Our findings indicate that LPE not only inhibits the production of UA but also promotes its elimination, positioning it as a promising candidate for UA-lowering agents.
Identification of AKNA Gene and Its Role for Genetic Susceptibility in Epithelial Ovarian Cancer
Authors: Suryandari Dwi Anita , Miftahuzzakiyah Miftahuzzakiyah , Yunaini Luluk , Kodariah Ria , Sukmawati Dewi , Rustamadji Primariadewi , Sari Puji , Ningsih Sri Suciati
Published: January 2025

AKNA is identified as a gene that regulates inflammation, immune response, and Epithelial–Mesenchymal Transition (EMT), which plays an important role in the progression of epithelial ovarian cancer. In this study, we analyzed the genotype and allele distribution as well as 3D modeling of one of the AKNA rs10817595 (−1372 C>A). The distribution of genotypes and alleles was analyzed using the T-ARMS PCR method on 63 ovarian cancer samples and 65 controls. AKNA mRNA expression was analyzed using qRT-PCR on 35 low-grade and 28 high-grade samples. Fifteen low-grade and 12 high-grade samples were analyzed for AKNA protein levels using immunohistochemistry. A 3D model of protein structure was constructed using AlphaFold. Significant differences in AKNA protein levels were found. However, no significant correlation was found for relative AKNA mRNA expression with protein levels. This result is thought to be related to decreased immune system response, increased inflammation, and increased EMT in epithelial ovarian cancer. AKNA gene variant (−1372 C>A) can cause a decrease in mRNA and protein levels in the low-grade and high-grade groups, so it has the potential as a genetic susceptibility factor in epithelial ovarian cancer.
Cranberry: A Promising Natural Product for Animal Health and Performance
Authors: Prasad Sahdeo , Patel Bhaumik , Kumar Prafulla , Mitra Pranabendu , Lall Rajiv
Published: January 2025

Cranberries are a distinctive source of bioactive compounds, containing polyphenols such as flavonoids, anthocyanins, phenolic acids, and triterpenoids. Cranberries are often associated with potential health benefits for the urinary tract and digestive system due to their high antioxidant, anti-inflammatory, antimicrobial, and immunomodulatory properties. Cranberry induces the production of antioxidant enzymes, suppresses lipid peroxidation, reduces inflammatory cytokines, modulates immune cells, maintains gut microbiota, and inhibits bacterial adhesion and growth. Cranberry polyphenols also have metal-binding motifs that bind with metals, particularly zinc and iron. The combination of cranberry polyphenols and metals displays increased biological activity. In this review, an attempt is made to describe the physiological properties and health benefits of cranberries for livestock, including poultry, swine, canine, feline, and ruminant animals, as either feed/food or as supplements. Cranberry, and/or its components, has the capability to potentially control infectious diseases like diarrhea, urinary tract infection, gut integrity, and intestinal probiotic health. Moreover, cranberries show efficacy in suppressing the growth of pathogenic microorganisms such as Salmonella species, Campylobacter species, Streptococcus species, and Enterococcus species bacteria. Thus, cranberry could be considered as a potential natural feed additive or food supplement for animal health improvement.
Tree Crown Damage and Physiological Responses Under Extreme Heatwave in Heterogeneous Urban Habitat of Central China
Authors: Zhang Li , Zhu Wenli , Zhang Ming , Xing Xiaoyi
Published: January 2025

(1) Background: Global warming has intensified dry heatwaves, threatening urban tree health and ecosystem services. Crown damage in trees is a key indicator of heat stress, linked to physiological changes and urban habitat characteristics, but the specific mechanisms remain to be explored. (2) Methods: This study investigated the heatwave-induced crown damage of Wuhan’s urban tree species, focusing on the influence of physiological responses and urban habitats. Crown damage was visually scored, and physiological responses were measured via stomatal conductance (Gs) and transpiration rate (Tr). (3) Results: Significant interspecific differences in crown damage were identified, with Prunus × yedoensis showing the highest degree of crown damage, while Pittosporum tobira displayed the lowest. A strong correlation was observed between crown damage and Gs and Tr, albeit with species-specific variations. The Degree of Building Enclosure (DegBE) emerged as the most prominent habitat factor, with a mitigating effect on crown damage, followed by the Percentage of Canopy Coverage (PerCC), in contrast with the Percentage of Impermeable Surface (PerIS) that showed a significant positive correlation. (4) Conclusions: The above findings suggest that species traits and habitat configurations interact in complex ways to shape tree resilience under heatwave stress, informing strategies for urban vegetation protection against heat stress in Central Chinese cities.
The First Detection of Parasite Ellobiopsis sp. on Calanoids (Crustacea: Copepoda) Inhabiting the Caspian Sea (Central Asia: West Kazakhstan)
Authors: Aubakirova Moldir , Assylbekova Saule Zh. , Isbekov Kuanysh B. , Kim Arkady , Zhaksylykova Ainur A. , Bolatbekova Zamira
Published: January 2025

The data on hosts of Ellobiopsis in Central Asia waterbodies are nearly non-existent. All research in this direction was conducted in other regions (Europe and Brazil). Parasitological studies were carried out in different seasons in the North and Middle Caspian Sea. Twenty-one taxa were registered in zooplankton, and only dominants of community calanoids Acartia (Acanthacartia) tonsa and Calanipeda aquaedulcis were infected with Ellobiopsis sp. Calanoida C. aquaedulcis was reported for the first time as a host for Ellobiopsis. The number of parasites per host was equal to one. The body length of parasites varied from 0.10 to 0.80 mm. The highest infection degree was recorded in C. aquaedulcis (5.71%), and it varied from 2.61% to 3.35% in Acartia. The individuals in the juvenile developmental stages were infected in Calanipeda, while in Acartia, individuals in all developmental stages were vulnerable to infection. The infected calanids had reduced body lengths. The findings suggest the possible influence of Ellobiopsis sp. on quantitative variables of hosts in the Middle Caspian, especially on biomass, by reducing the body sizes of hosts. However, no effect on the abundance and biomass of the host and the structure of the zooplankton of the North Caspian Sea has been detected.
Exploring Endophytic Fungi from Humulus lupulus L. for Biocontrol of Phytopathogenic Fungi
Authors: Riccioni Claudia , Belfiori Beatrice , Cenci Maurizio , Rubini Andrea
Published: January 2025

Humulus lupulus L. (hop) is a crucial crop within the brewing industry and a rich source of bioactive compounds. Traditionally concentrated in northeast regions of Europe, hop cultivation has expanded towards southern territories such as Italy over recent decades. Managing phytosanitary threats in Mediterranean climates poses challenges due to limited knowledge and registered agrochemicals. In pursuit of eco-friendly alternatives for disease management, we isolated 262 endophytic fungal strains from wild hop roots, stems, leaves, and flowers. Through phylogenetic analyses, we identified 51 operational taxonomic units. Dominant species such as Ilyonectria macrodidyma, Penicillium sp., Diaporthe columnaris, Plectosphaerella cucumerina, and Fusarium oxysporum were exclusive to roots. In contrast, Alternaria spp. and Epicoccum spp. were prevalent in other tissues, and Botrytis cinerea was exclusively detected in female flowers. We tested seven isolates—Epicoccum sp., Aureobasidium pullulans, Plectosphaerella cucumerina, Stemphylium vesicarium, Periconia byssoides, Talaromyces wortmannii, and Nigrospora sphaerica—against the four phytopathogenic fungi Alternaria sp., Fusarium oxysporum, Botrytis cinerea, and Sclerotinia sclerotiorum. All endophytes exhibited antagonistic effects against at least one pathogen, with Plectosphaerella cucumerina showing the strongest inhibition against Alternaria sp. This study marks the first exploration of endophytic fungi from various hop tissues. All isolated strains were ex situ conserved for future bioactivity assessments and biotechnological applications. Original data with a key relevance for the environmentally friendly management of plant diseases are provided.
Global Dataset of Extreme Sea Levels and Coastal Flood Impacts over the 21st Century
Authors: Kirezci Ebru , Young Ian , Ranasinghe Roshanka , Chen Yiqun , Zhang Yibo , Rajabifard Abbas
Published: January 2025

A global database of coastal flooding impacts resulting from extreme sea levels is developed for the present day and for the years 2050 and 2100. The database consists of three sub-datasets: the extreme sea levels, the coastal areas flooded by these extreme sea levels, and the resulting socioeconomic implications. The extreme sea levels consider the processes of storm surge, tide levels, breaking wave setup and relative sea level rise. The socioeconomic implications are expressed in terms of Expected Annual Population Affected (EAPA) and Expected Annual Damage (EAD), and presented at the global, regional and national scales. The EAPA and EAD are determined both for existing coastal defence levels and assuming two plausible adaptation scenarios, along with socioeconomic development narratives. All the sub-datasets can be visualized with a Digital Twin platform based on a GIS-based mapping host. This publicly available database provides a first-pass assessment, enabling users to extract and identify global and national coastal hotspots under different projections of sea level rise and socioeconomic developments.
Primary Congenital and Childhood Glaucoma—A Complex Clinical Picture and Surgical Management
Authors: Coviltir Valeria , Marinescu Maria Cristina , Urse Bianca Maria , Burcel Miruna Gabriela
Published: January 2025

Childhood glaucoma encompasses a group of rare but severe ocular disorders characterized by increased intraocular pressure (IOP), posing significant risks to vision and quality of life. Primary congenital glaucoma has a prevalence of one in 10,000–68,000 people in Western countries. More worryingly, it is responsible for 5–18% of all childhood blindness cases. According to the Childhood Glaucoma Research Network (CGRN), this spectrum of disease is classified into primary glaucoma (primary congenital glaucoma and juvenile open-angle glaucoma) and secondary glaucomas (associated with non-acquired ocular anomalies, non-acquired systemic disease, acquired conditions, and glaucoma after cataract surgery). They present very specific ocular characteristics, such as buphthalmos or progressive myopic shift, corneal modifications such as Haab striae, corneal edema or increased corneal diameter, and also glaucoma findings including high intraocular pressure, specific visual fields abnormalities, and optic nerve damage such as increased cup-disc ratio, cup-disc ratio asymmetry of at least 0.2 and focal rim thinning. Surgical intervention remains the cornerstone of treatment, and initial surgical options include angle surgeries such as goniotomy and trabeculotomy, aimed at improving aqueous outflow. For refractory cases, trabeculectomy and glaucoma drainage devices (GDDs) serve as second-line therapies. Advanced cases may require cyclodestructive procedures, including transscleral cyclophotocoagulation, reserved for eyes with limited visual potential. All in all, with appropriate management, the prognosis of PCG may be quite favorable: stationary disease has been reported in 90.3% of cases after one year, with a median visual acuity in the better eye of 20/30. Immediate recognition of the specific signs and symptoms by caregivers, primary care providers, and ophthalmologists, followed by prompt diagnosis, comprehensive surgical planning, and involving the caregivers in the follow-up schedule remain critical for optimizing outcomes in childhood glaucoma management.
Exonic and Intronic WNT10A Variants Isolated from Korean Children with Non-Syndromic Tooth Agenesis
Authors: Ju Yeonjin , Lee Joo Yeon , Hwang Woochang , Shin Jonghyun , Kim Hyung-Sik , Hur Junho K. , Lee Eungyung
Published: January 2025

Background/Objectives: Tooth agenesis (TA) is a developmental anomaly prevalent in humans. It is particularly significant in children and adolescents because it is related to esthetic, physiological, and functional problems, including malocclusion, periodontal damage, and insufficient alveolar growth. WNT10A mutations have been identified as the main genetic alterations associated with tooth agenesis. Most previous studies have investigated WNT10A mutations in patients with tooth agenesis using single nucleotide polymorphism (SNP) arrays or exome sequencing. In this study, we conducted a comprehensive profiling of mutations within the exons and introns of WNT10A in Korean patients with non-syndromic tooth agenesis. Methods: Saliva samples were collected from Korean children and adolescents with non-syndromic tooth agenesis. Tagmentation-based sequencing was conducted to acquire mutation information for all exonic and intronic bases of the WNT10A gene. Results: Mutations were detected exclusively in the patient samples: 629C>G and 1100C>T in exon 1, 1977T>C in intron 1, 10256C>T and 10382G>A in exon 3, and 15953G>A in intron 4. Additional mutations were also observed at high ratios in the patient samples. Conclusions: The mutations identified in this study differ from previous findings. These results may provide useful information for understanding the pathogenicity of WNT10A mutations in Korean patients with tooth agenesis and support future diagnostic and therapeutic approaches.
Development and Experimental Validation of a Sense-and-Avoid System for a Mini-UAV
Authors: Fiorio Marco , Galatolo Roberto , Di Rito Gianpietro
Published: January 2025

This paper provides an overview of the three-year effort to design and implement a prototypical sense-and-avoid (SAA) system based on a multisensory architecture leveraging data fusion between optical and radar sensors. The work was carried out within the context of the Italian research project named TERSA (electrical and radar technologies for remotely piloted aircraft systems) undertaken by the University of Pisa in collaboration with its industrial partners, aimed at the design and development of a series of innovative technologies for remotely piloted aircraft systems of small scale (MTOW < 25 Kgf). The system leverages advanced computer vision algorithms and an extended Kalman filter to enhance obstacle detection and tracking capabilities. The “Sense” module processes environmental data through a radar and an electro-optical sensor, while the “Avoid” module utilizes efficient geometric algorithms for collision prediction and evasive maneuver computation. A novel hardware-in-the-loop (HIL) simulation environment was developed and used for validation, enabling the evaluation of closed-loop real-time interaction between the “Sense” and “Avoid” subsystems. Extensive numerical simulations and a flight test campaign demonstrate the system’s effectiveness in real-time detection and the avoidance of non-cooperative obstacles, ensuring compliance with UAV aero mechanical and safety constraints in terms of minimum separation requirements. The novelty of this research lies in (1) the design of an innovative and efficient visual processing pipeline tailored for SWaP-constrained mini-UAVs, (2) the formulation an EKF-based data fusion strategy integrating optical data with a custom-built Doppler radar, and (3) the development of a unique HIL simulation environment with realistic scenery generation for comprehensive system evaluation. The findings underscore the potential for deploying such advanced SAA systems in tactical UAV operations, significantly contributing to the safety of flight in non-segregated airspaces
NoctuDroneNet: Real-Time Semantic Segmentation of Nighttime UAV Imagery in Complex Environments
Authors: Qu Ruokun , Tan Jintao , Liu Yelu , Li Chenglong , Jiang Hui
Published: January 2025

Nighttime semantic segmentation represents a challenging frontier in computer vision, made particularly difficult by severe low-light conditions, pronounced noise, and complex illumination patterns. These challenges intensify when dealing with Unmanned Aerial Vehicle (UAV) imagery, where varying camera angles and altitudes compound the difficulty. In this paper, we introduce NoctuDroneNet (Nocturnal UAV Drone Network, hereinafter referred to as NoctuDroneNet), a real-time segmentation model tailored specifically for nighttime UAV scenarios. Our approach integrates convolution-based global reasoning with training-only semantic alignment modules to effectively handle diverse and extreme nighttime conditions. We construct a new dataset, NUI-Night, focusing on low-illumination UAV scenes to rigorously evaluate performance under conditions rarely represented in standard benchmarks. Beyond NUI-Night, we assess NoctuDroneNet on the Varied Drone Dataset (VDD), a normal-illumination UAV dataset, demonstrating the model’s robustness and adaptability to varying flight domains despite the lack of large-scale low-light UAV benchmarks. Furthermore, evaluations on the Night-City dataset confirm its scalability and applicability to complex nighttime urban environments. NoctuDroneNet achieves state-of-the-art performance on NUI-Night, surpassing strong real-time baselines in both segmentation accuracy and speed. Qualitative analyses highlight its resilience to under-/over-exposure and small-object detection, underscoring its potential for real-world applications like UAV emergency landings under minimal illumination.
Neuro-Fuzzy Network-Based Nonlinear Hybrid Active Noise Control Systems
Authors: Nguyen Thi Trung Tin , Na Jing , Nguyen Le Thai , Wang Xian
Published: January 2025

Active noise control (ANC) technology has extensive applications in suppressing sound pollution in the real-world environment. In this paper, a new adaptive neuro-fuzzy network (ANFN)-based controller is presented and integrated into hybrid active noise control (HANC) systems to improve the robustness and effectiveness of active noise suppression. Specifically, an adaptive neural network is constructed to minimize the mean square error information with respect to the residual noise. Moreover, a fuzzy logic strategy is proposed to address the manual fine-tuning and nonlinearities encountered in a complex environment. Finally, the stability of the proposed control method is proved by using the Lyapunov theorem. Comparative numerical simulations are given to verify the effectiveness and superiority of the proposed method under different noise signals.
Finite-Size Scaling in the Ageing Dynamics of the 1D Glauber–Ising Model
Authors: Henkel Malte
Published: January 2025

Single-time and two-time correlators are computed exactly in the 1D Glauber-Ising model after a quench to zero temperature and on a periodic chain of finite length N, using a simple analytical continuation technique. Besides the general confirmation of finite-size scaling in non-equilibrium dynamics, this allows for testing the scaling behaviour of the plateau height C∞(2), to which the two-time auto-correlator converges when deep in the finite-size regime.
“Video Killed the Radio Star”: Transitioning from an Audio- to a Video-Based Exam in Hungarian Language Classes for International Medical Students
Authors: Hild Gabriella , Dávidovics Anna , Warta Vilmos , Németh Timea
Published: January 2025

This action research examines the transition from audio- to video-based tasks in the final Medical Hungarian exam for international medical students, aiming to better align assessment with real-life language needs and enhance student motivation. Conducted at a Hungarian medical university with 61 second-year students, the study uses a mixed-methods approach. Quantitative data from a questionnaire and qualitative insights from focus group interviews reveal students’ experiences with the video-based exam tasks and preparatory materials. The results indicate a positive reception of the Practice Test Book and the new video exam format, with visual cues like body language aiding in comprehension and engagement. Students found that the video-based tasks closely mirrored clinical interactions, strengthening the relevance of language skills in professional contexts. Preparatory materials, including lead-in exercises, were well-received by students and seen as effective in improving readiness for the exam. The study suggests that the shift from audio- to video-based assessment can bridge classroom learning with real-world application, potentially serving as a model for other non-traditional study abroad settings in Languages Other Than English (LOTEs), especially as purely audio-based communication has become less prevalent in today’s world.
Valorization of Onion By-Products Bioactive Compounds by Spray Drying Encapsulation Technique
Authors: Flamminii Federica , D’Alessio Giulia , Chiarini Marco , Di Michele Alessandro , De Bruno Alessandra , Mastrocola Dino , Di Mattia Carla Daniela
Published: January 2025

The increasing interest in sustainability has driven research into the utilization of food by-products. Onion by-products, rich in bioactive compounds, represent a valuable resource for developing functional ingredients; however, they are prone to degradation due to environmental factors such as light, heat, and oxygen, leading to reduced efficacy and increased spoilage. Microencapsulation represents an effective approach to meet important goals in the formulation of food products such as the protection against degradation or the control of interactions with other ingredients that may modify and impair their functionality. This study explores the microencapsulation of flavonoid-rich onion by-product extract through spray drying, employing various wall materials (maltodextrin and a mixture of maltodextrin/trehalose and maltodextrin/trehalose/inulin) and their effect on the chemical and physical properties of the powders such as encapsulation efficiency, total flavonoids content, moisture content, water activity, bulk density, and bulk tapped density. The storage stability was further evaluated. This research supports waste reduction and suggests strategies for developing functional ingredients with extended shelf life and controlled release properties.
A Comparative Elemental Analysis of Espresso Coffee from Poland and Portugal
Authors: Konieczyński Pawel , Seroczyńska Kinga , Wesolowski Marek , Pinto Edgar , Couto Cristina , Cunha Ana , Azevedo Rui , Almeida Agostinho
Published: January 2025

A comparative elemental analysis of espresso coffee from Poland and Portugal was carried out. Using an ICP-MS analytical procedure, samples collected from public cafes in Poland and Portugal (n = 60 and n = 44, respectively) were studied for their macromineral and trace element content. To evaluate the contribution of water to the final composition of the beverage, paired samples (i.e., collected from the same locations) of drinking water were also analysed. The mineral profile of the coffee espresso samples was quite similar: Mg > P > Ca > Rb > Mn > B > Zn > Cu > Sr > Ba > Ni > Pb > Cs > Mo > Sn > Cd > Sb > Tl for samples from Poland and Mg > P > Ca > Rb > B > Mn > Zn > Sr > Cu > Ni > Ba > Cs > Pb > Mo > Sn > Sb > Cd > Tl for samples from Portugal. For most of the elements, the espresso samples showed much higher levels than the water used in its preparation. The two most notable exceptions were Ca and Sr, where the elements present in the coffee came mainly from the water. The contribution of coffee espressos to the daily intake of essential elements seems to be reduced. Other non-essential elements like Ni (median = 81.0 µg/L and 86.8 µg/L for Polish and Portuguese espresso, respectively) and Pb (median = 14.3 µg/L and 4.43 µg/L, respectively) were observed in significant amounts in the coffee espresso samples analysed in this study. These elements have been shown to leach from coffee machines in other studies. More studies are necessary to confirm these results.