bluuebunny/crossref_metadata_2025 huggingface dataset:
Non-Alcoholic Fatty Liver Disease (NAFLD) Is an Independent Risk Factor for Developing New-Onset Diabetes After Acute Pancreatitis: A Multicenter Retrospective Cohort Study in Chinese Population
Authors: Lv Yingqi , Zhang Jun , Yang Ting , Sun Jinfang , Hou Jiaying , Chen Zhiwei , Yu Xuehua , Yuan Xuelu , Lu Xuejia , Xie Ting , Yu Ting , Su Xianghui , Liu Gaifang , Zhang Chi , Li Ling
Published: May 2022

Background

Numerous studies validated frequent glucose dysfunction in patients with acute pancreatitis (AP). However, the prevalence of new-onset diabetes in individuals after a first episode of AP varies widely among previous studies. This study aims to determine the incidence of post-acute pancreatitis diabetes mellitus (PPDM-A) in Chinese people and further identify potential risk factors that influence diabetes development in patients with AP.

Methods

This was a multi-center retrospective cohort study including 6009 inpatients with a first attack of AP. A total of 1804 patients with AP without known endocrine pancreatic disorders or other pancreatic exocrine diseases were eligible for analysis. Data was collected from medical records by hospital information system and telephone follow-ups after discharge. The multiple logistic regression analysis was established to evaluate the potential influencing factors of PPDM-A.

Results

The prevalence of newly diagnosed diabetes after a first episode of AP in China was 6.2%. Data showed that patients who developed PPDM-A were more likely to be younger (X2 = 6.329, P = 0.012), experienced longer hospital stays (X2 = 6.949, P = 0.008) and had a higher frequency of overweight or obesity (X2 = 11.559, P = 0.003) compared to those with normal glycemia. The frequency of stress hyperglycemia on admission (X2 = 53.815, P < 0.001), hyperlipidemia (X2 = 33.594, P < 0.001) and non-alcoholic fatty liver disease (NAFLD) (X2 = 36.335, P < 0.001) were significantly higher among individuals with PPDM-A compared with control group. Also, patients with PPDM-A were more likely to be hyperlipidemic AP (X2 = 16.304, P = 0.001) and show a higher degree of severity (X2 = 7.834, P = 0.020) and recurrence rate (X2 = 26.908, P < 0.001) of AP compared to those without diabetes. In addition, multiple logistic regression analysis indicated that stress hyperglycemia, hyperlipidemia, NAFLD and repeated attacks of AP were the independent influence factors for developing PPDM-A.

Conclusion

Our study first demonstrated the prevalence of secondary diabetes in Chinese patients after AP. The disorder of glucose metabolism in individuals with AP should be regularly evaluated in clinical practice. Further studies are needed to verify the relationship between liver and pancreas in keeping glucose homeostasis under AP condition.
A Review on the Rural Household Energy in China From 1990s—Transition, Regional Heterogeneity, Emissions, Energy-Saving, and Policy
Authors: Zhang Xinxin , Xu Kaili , He Maogang , Wang Jingfu
Published: May 2022

Rural energy is related to the domestic energy supply, consumption, and improvement of living standards of more than one-third of the population in China. In the “14th Five-Year Plan,” it has been clearly pointed out that it is necessary to strengthen the clean utilization of coal and implement the construction of rural clean energy projects. At present, the energy consumption structure of rural areas in China is transiting from traditional solid energy to commercial energy and clean energy. Based on this background, this paper reviews the transition trend, influencing factors, and regional differences of China’s rural household energy consumption structure from the 1990s. Taking into account China’s goal of carbon peaking by 2030 and carbon neutrality by 2060, carbon dioxide and pollutant emissions generated in the process of energy consumption and the energy-saving potential of rural households are analyzed and discussed. Moreover, the evolution of rural energy policies in China is presented and related proposals are also made. This review aims to provide reference for relevant researchers and policy makers.
A State-of-Charge-Based Flexible Synthetic Inertial Control Strategy of Battery Energy Storage Systems
Authors: You Feng , Si Xiuli , Dong Rong , Lin Dong , Xu Yien , Xu Yiming
Published: May 2022

Power systems would face issues in system frequency stability when high scales of variable renewable energy generation are integrated in them. Battery energy storage systems (BESSs) with advanced control capability and rapid control response have become a countermeasure to solve the issues in system frequency stability. This research addresses a flexible synthetic inertial control strategy of the BESS to enhance the dynamic system frequency indices including the frequency nadir, settling frequency, and rate of change of the system frequency. To this end, the control loops based on the frequency excursion and rate of change of the system frequency are implemented into the d-axis controller of the BESS. The adaptive control coefficient of both control loops could be adjusted according to the instantaneous state of charge (SOC) so that it can inject more power to the grid at a higher SOC. The benefits of the proposed combined inertial control strategy are investigated with various sizes of disturbance and SOCs of the BESSs. Results successfully illustrate that the proposed combined inertial control strategy of the BESS is capable of enhancing the system frequency stability so as to promote variable renewable energy accommodation.
Parameter Identification of Electromechanical Oscillation Mode in Power Systems Driven by Data: A Quasi-Real-Time Method Based on Randomized-DMD-Multilayer Artificial Neural Networks
Authors: Cai Guowei , Guo Shujia , Liu Cheng
Published: May 2022

With the increase in the power system scale, the identification of electromechanical oscillation mode parameters by traditional numerical methods can no longer meet the requirements of complete real-time analysis. Therefore, a method based on machine learning (multilayer artificial neural networks) is proposed to identify the electromechanical oscillation mode parameters of the power system. This method can take the monitorable variables of the WAMS as the input of the model and the key characteristic information such as frequency and damping ratio as the output. After processing the input and output data with randomized dynamic mode decomposition (randomized-DMD), their mapping relationship can be analyzed by using the multilayer neuron architecture. The simulation results of the 4-generator 2-area system and the IEEE 16-generator 5-area system show that this method can accurately calculate the key characteristic parameters of the system without considering the change in the control parameters and after the offline training of historical data, which shows higher accuracy, stronger robustness, and sensitive online tracking ability.
Remote Sensing and Nuclear Techniques for Soil Erosion Research in Forest Areas: Case Study of the Crveni Potok Catchment
Authors: Manić Miloš , Đorđević Milan , Đokić Mrđan , Dragović Ranko , Kićović Dušan , Đorđević Dejan , Jović Mihajlo , Smičiklas Ivana , Dragović Snežana
Published: May 2022

Hilly terrains characterized by the looseness of the surface soil layer and the slope of the topographic surface are subjected to diverse erosion processes, which could greatly impact forest management. An erosive process was identified along the unstable slopes in most of the Crveni potok catchment in the southeastern part of Serbia. The results of this study indicate two erosion types of variable intensity, dominated by weak and moderate erosion, while severe erosion is identified at several locations. Water pressure in the pores of loose soil in the Crveni potok catchment generates gully erosion in parts of the catchment that are geologically predisposed by the composition of the parent substrate. The paper discusses the factors influencing gully erosion, such as soil density, the slope of the topographic surface, rainfall (precipitation amount, annual distribution), and vegetation characteristics. The set of used methodologies, including remote sensing, nuclear, and geospatial ones, provided an integrated assessment of factors affecting erosion in unstable forest areas. The soil erosion rates in the study area are estimated from inventories of fallout radionuclide 137Cs using conversion models. Airborne photogrammetry, terrestrial Structure-from-Motion 360-degree camera photogrammetry, and Terrestrial LiDAR Scanning (TLS) are used to model complex geomorphological features of forest terrain. These techniques are found to be convenient for the quantification of the existing state of erosion (length, width, volume, and density of the gully systems) in the forest gullies. The remote sensing techniques provided an insight into the physiographic characteristics of the studied area important for determining its parts affected by erosion. The models obtained using different techniques are compared, techniques are evaluated, and their advantages and disadvantages are pointed out. This approach enables the identification of priority targets for soil conservation and site-specific remediation measures to foster sustainable forest management.
The Effects of Drainage on the Soil Fungal Community in Freshwater Wetlands
Authors: Zhao Qingqing , Bai Junhong , Jia Jia , Zhang Guangliang , Wang Jianing , Gao Yongchao
Published: May 2022

Wetland drainage has been intensively implemented globally, and it has exerted significant effects on wetland ecosystems. The effects of wetland drainage on the soil fungal community remain to be clarified. Soil samples were collected at depths of 0–5 and 5–10 cm in freshwater Phragmites australis wetlands to investigate changes in the fungal community before and after drainage (termed FW and DFW, respectively) using high-throughput sequencing of the fungal-specific internal transcribed spacer 1 (ITS1) gene region. No significant differences in the α diversity of the soil fungal community were found in 0–10 cm soils between FW and DFW (p > 0.05), except for the abundance-based coverage estimator (ACE) and Chao1 indices in 5–10 cm soils. Significantly higher values of ACE and Chao1 in 5–10 cm soils in FW than in DFW indicated that wetland drainage may reduce fungal community richness in 5–10 cm soils. Ascomycota, Sordariomycetes, and Cephalothecaceae were the dominant fungal phylum, class, and family, respectively, in 0–5 and 5–10 cm soils of both FW and DFW, representing as high as 76.17, 58.22, and 45.21% of the fungal community in 5–10 FW soils, respectively. Saprotrophic fungi predominated in both FW and DFW. Drainage altered both the fungal community structure and some edaphic factors. Mantel tests and Spearman correlation analyses implied that edaphic factors [i.e., soil organic matter (SOM), electronic conductivity (EC), pH, and clay] also affected soil fungal community structure. Overall, wetland drainage altered the community structure of the fungal community in the freshwater wetlands.
Patients With Inflammatory Bowel Disease Show IgG Immune Responses Towards Specific Intestinal Bacterial Genera
Authors: Bourgonje Arno R. , Roo-Brand Geesje , Lisotto Paola , Sadaghian Sadabad Mehdi , Reitsema Rosanne D. , de Goffau Marcus C. , Faber Klaas Nico , Dijkstra Gerard , Harmsen Hermie J. M.
Published: May 2022

Introduction

Inflammatory bowel disease (IBD) is characterized by a disturbed gut microbiota composition. Patients with IBD have both elevated mucosal and serum levels of IgG-antibodies directed against bacterial antigens, including flagellins. In this study, we aimed to determine to which intestinal bacteria the humoral immune response is directed to in patients with IBD.

Methods

Fecal and serum samples were collected from patients with IBD (n=55) and age- and sex-matched healthy controls (n=55). Fecal samples were incubated with autologous serum and IgG-coated fractions were isolated by magnetic-activated cell sorting (MACS) and its efficiency was assessed by flow cytometry. The bacterial composition of both untreated and IgG-coated fecal samples was determined by 16S rRNA-gene Illumina sequencing.

Results

IgG-coated fecal samples were characterized by significantly lower microbial diversity compared to the fecal microbiome. Both in patients with IBD and controls, serum IgG responses were primarily directed to Streptococcus, Lactobacillus, Lactococcus, Enterococcus, Veillonella and Enterobacteriaceae, as well as against specific Lachnospiraceae bacteria, including Coprococcus and Dorea (all P<0.001), and to Ruminococcus gnavus-like bacteria (P<0.05). In contrast, serological IgG responses against typical commensal, anaerobic and colonic microbial species were rather low, e.g. to the Lachnospiraceae members Roseburia and Blautia, to Faecalibacterium, as well as to Bacteroides. Patients with IBD showed more IgG-coating of Streptococcus, Lactobacillus, and Lactococcus bacteria compared to healthy controls (all P<0.05). No differences in IgG-coated bacterial fractions were observed between Crohn’s disease and ulcerative colitis, between active or non-active disease, nor between different disease locations.

Conclusion

The IgG immune response is specifically targeted at distinct intestinal bacterial genera that are typically associated with the small intestinal microbiota, whereas responses against more colonic-type commensals are lower, which was particularly the case for patients with IBD. These findings may be indicative of a strong immunological exposure to potentially pathogenic intestinal bacteria in concordance with relative immune tolerance against commensal bacteria.
Impact of Human Genetic Variation on C-Reactive Protein Concentrations and Acute Appendicitis
Authors: Ricaño-Ponce Isis , Peeters Toon , Matzaraki Vasiliki , Houben Bert , Achten Ruth , Cools Peter , Netea Mihai G. , Gyssens Inge C. , Kumar Vinod
Published: May 2022

Background

Acute appendicitis is one of the most common abdominal emergencies worldwide. Both environmental and genetic factors contribute to the disease. C-reactive protein (CRP) is an important biomarker in the diagnosis of acute appendicitis. CRP concentrations are significantly affected by genetic variation. However, whether such genetic variation is causally related to appendicitis risk remains unclear. In this study, the causal relationship between single-nucleotide polymorphisms (SNPs) associated with circulating CRP concentrations and the risk and severity of acute appendicitis was investigated.

Methods

CRP concentrations in serum of appendicitis patients (n = 325) were measured. Appendicitis was categorized as complicated/uncomplicated and gangrenous/non-gangrenous. Imputed SNP data (n = 287) were generated. A genome-wide association study (GWAS) on CRP concentrations and appendicitis severity was performed. Intersection and colocalization of the GWAS results were performed with appendicitis and CRP-associated loci from the Pan-UKBB cohort. A functional-genomics approach to prioritize genes was employed.

Results

Thirteen percent of significant CRP quantitative trait loci (QTLs) that were previously identified in a large cohort of healthy individuals were replicated in our small patient cohort. Significant enrichment of CRP-QTLs in association with appendicitis was observed. Among these shared loci, the two top loci at chromosomes 1q41 and 8p23.1 were characterized. The top SNP at chromosome 1q41 is located within the promoter of H2.0 Like Homeobox (HLX) gene, which is involved in blood cell differentiation, and liver and gut organogeneses. The expression of HLX is increased in the appendix of appendicitis patients compared to controls. The locus at 8p23.1 contains multiple genes, including cathepsin B (CTSB), which is overexpressed in appendix tissue from appendicitis patients. The risk allele of the top SNP in this locus also increases CTSB expression in the sigmoid colon of healthy individuals. CTSB is involved in collagen degradation, MHC class II antigen presentation, and neutrophil degranulation.

Conclusions

The results of this study prioritize HLX and CTSB as potential causal genes for appendicitis and suggest a shared genetic mechanism between appendicitis and CRP concentrations.
SAPHO Syndrome Complicated by Ankylosing Spondylitis Successfully Treated With Tofacitinib: A Case Report
Authors: Yuan Fangfang , Luo Jing , Yang Qiong
Published: May 2022

Synovitis, acne, pustulosis, hyperostosis, and osteitis (SAPHO) syndrome, a type of chronic inflammatory disease, is rare and difficult to treat. Osteoarthropathy with skin involvement is the primary clinical manifestation of SAPHO syndrome. The unknown pathogenesis of SAPHO syndrome is speculated to be related to individual genetic differences, immune levels, microorganisms, and environmental factors. Tofacitinib, a novel small-molecule Janus kinase (JAK) inhibitor, has been used to treat rheumatoid arthritis. However, it also has great potential for the treatment of other immune diseases, including SAPHO syndrome. A 36-year-old man with chest and back pain for more than two months was admitted to our hospital. After admission, the patient developed a pustular rash and enteritis. SAPHO syndrome was diagnosed based on the above clinical manifestations, computed tomography (CT), and bone scintigraphy findings. Notably, the patient also had ankylosing spondylitis. Tofacitinib significantly improved the patient’s skin symptoms while preventing worsening of chest and back pain when adalimumab was discontinued. We report the first case of ankylosing spondylitis with SAPHO syndrome. In addition, it is also the first successful treatment thereof with tofacitinib. We hope to provide valuable information regarding the pathogenesis and treatment of SAPHO syndrome in this case.
Coarsening Evolution of γ′ Phase and Failure Mechanism of Co-Ni-Al-Ti-Based Superalloys During Isothermal Aging
Authors: Zhang Xuming , Shang Hang , Gao Qiuzhi , Ma Qingshuang , Zhang Hailian , Li Huijun , Sun Linlin
Published: May 2022

Novel Co-based superalloys, as potentially ideal aero-engine hot section materials, have a higher temperature bearing capacity and better oxidation resistance than Ni-based superalloys. Coarsening evolution of γ′ phase and failure mechanism of Co-Ni-Al-Ti-based superalloys during the isothermal aging process at 1073 K were investigated using multiple characterizations and testing methods. The results show that γ′ phase is uniformly distributed on the γ phase matrix, and coarsening with the increase in isothermal aging time, which results in a decrease in maximum tensile strength. Furthermore, Mo element is preferred to distribute in γ′ phase and provides stronger solution strengthening effect than Cr element, which determines more excellent mechanical properties of 2Mo superalloy than that of 2Cr superalloy. The coarsening rate of γ′ phase in the 2Cr superalloy is significantly higher than that in the 2Mo superalloy. Grain boundary failure is dominant in isothermal aging, and the cracks nucleate and expand along the vertical direction of loading stress on the grain boundary. The current work suggests that the coarsening of the γ′ phase, reduction in the volume fraction of γ′ phase, and formation of ′-precipitate depleted zone (PDZ) near the grain boundary during aging controls the deterioration of mechanical properties in Co-Ni-Al-Ti-based superalloys.
Mechanics and Strategies for Wrinkling Suppression: A Review
Authors: Zhang Zhaojie , Zhao Lingyu , Shi Jidong
Published: May 2022

Wrinkling is a well-known phenomenon observed at various length scales for diverse materials. Despite a variety of research studies focusing on investigating wrinkling mechanisms and utilizing them to create surface patterns recently, wrinkling also brings about the loss of specific functional properties that were initially endowed to the thin films. For instance, wrinkling may result in the degradation of electrical and optical properties of the film; and the wrinkled films are easily delaminated. Therefore, it is still meaningful to find ways for the suppression of wrinkling on the thin film, although the relevant works are far less than those that utilize the surface instability to achieve certain structures. In this review, the approaches to restrain the emergence of wrinkles will be introduced. Following the introduction part, numerical analysis for wrinkle generation will be first discussed, by which the key parameters determining wrinkle initiation and morphology will be provided. Then, wrinkle suppression strategies by tailoring these parameters will be introduced in the following sections. This review aims to provide useful guidance for future research on alleviating surface fluctuations and achieving desired device functionality.
A Review of the Diagnosis and Treatment of Limbal Stem Cell Deficiency
Authors: Kate Anahita , Basu Sayan
Published: May 2022

Limbal stem cell deficiency (LSCD) can cause significant corneal vascularization and scarring and often results in serious visual morbidity. An early and accurate diagnosis can help prevent the same with a timely and appropriate intervention. This review aims to provide an understanding of the different diagnostic tools and presents an algorithmic approach to the management based on a comprehensive clinical examination. Although the diagnosis of LSCD usually relies on the clinical findings, they can be subjective and non-specific. In such cases, using an investigative modality offers an objective method of confirming the diagnosis. Several diagnostic tools have been described in literature, each having its own advantages and limitations. Impression cytology and in vivo confocal microscopy (IVCM) aid in the diagnosis of LSCD by detecting the presence of goblet cells. With immunohistochemistry, impression cytology can help in confirming the corneal or conjunctival source of epithelium. Both IVCM and anterior segment optical coherence tomography can help supplement the diagnosis of LSCD by characterizing the corneal and limbal epithelial changes. Once the diagnosis is established, one of various surgical techniques can be adopted for the treatment of LSCD. These surgeries aim to provide a new source of corneal epithelial stem cells and help in restoring the stability of the ocular surface. The choice of procedure depends on several factors including the involvement of the ocular adnexa, presence of systemic co-morbidities, status of the fellow eye and the comfort level of the surgeon. In LSCD with wet ocular surfaces, autologous and allogeneic limbal stem cell transplantation is preferred in unilateral and bilateral cases, respectively. Another approach in bilateral LSCD with wet ocular surfaces is the use of an autologous stem cell source of a different epithelial lineage, like oral or nasal mucosa. In eyes with bilateral LSCD with significant adnexal issues, a keratoprosthesis is the only viable option. This review provides an overview on the diagnosis and treatment of LSCD, which will help the clinician choose the best option amongst all the therapeutic modalities currently available and gives a clinical perspective on customizing the treatment for each individual case.
Potential Prognostic Indicators for Patients With Retinal Vein Occlusion
Authors: Yin Shan , Cui Yanyan , Jiao Wanzhen , Zhao Bojun
Published: May 2022

The second most prevalent cause of retinal vascular disease is retinal vein occlusion (RVO). RVO raises intravascular pressure in the capillary and veins, triggering vessel barrier collapse and subsequent leaking of blood or plasma components into the tissue (edema). Macular edema (ME) is a major complication of RVO that results in significant visual impairment. Laser therapy, intravitreal steroid injections, and vascular endothelial growth factor (VEGF) inhibitors are the major therapeutic techniques. Different therapies reduce ME of RVO and improve visual activity. However, some people have no impact on the resolution of ME, while others have a poor visual prognosis despite full ME cure. There are many investigators who studied the relationship between indicators of various instruments with visual activity. However, a summary of those findings is currently lacking. Therefore, we will focus on the predictive factors of different studies associated with positive visual activity outcomes, which would be very useful and important to help address both treatment expectations and methods for patients with RVO.
Raman Study of Pathogenic Candida auris: Imaging Metabolic Machineries in Reaction to Antifungal Drugs
Authors: Pezzotti Giuseppe , Kobara Miyuki , Nakaya Tamaki , Imamura Hayata , Asai Tenma , Miyamoto Nao , Adachi Tetsuya , Yamamoto Toshiro , Kanamura Narisato , Ohgitani Eriko , Marin Elia , Zhu Wenliang , Nishimura Ichiro , Mazda Osam , Nakata Tetsuo , Makimura Koichi
Published: May 2022

The multidrug-resistant Candida auris often defies treatments and presently represents a worldwide public health threat. Currently, the ergosterol-targeting Amphotericin B (AmB) and the DNA/RNA-synthesis inhibitor 5-flucytosine (5-FC) are the two main drugs available for first-line defense against life-threatening Candida auris infections. However, important aspects of their mechanisms of action require further clarification, especially regarding metabolic reactions of yeast cells. Here, we applied Raman spectroscopy empowered with specifically tailored machine-learning algorithms to monitor and to image in situ the susceptibility of two Candida auris clades to different antifungal drugs (LSEM 0643 or JCM15448T, belonging to the East Asian Clade II; and, LSEM 3673 belonging to the South African Clade III). Raman characterizations provided new details on the mechanisms of action against Candida auris Clades II and III, while also unfolding differences in their metabolic reactions to different drugs. AmB treatment induced biofilm formation in both clades, but the formed biofilms showed different structures: a dense and continuous biofilm structure in Clade II, and an extra-cellular matrix with a “fluffy” and discontinuous structure in Clade III. Treatment with 5-FC caused no biofilm formation but yeast-to-hyphal or pseudo-hyphal morphogenesis in both clades. Clade III showed a superior capacity in reducing membrane permeability to the drug through chemically tailoring chitin structure with a high degree of acetylation and fatty acids networks with significantly elongated chains. This study shows the suitability of the in situ Raman method in characterizing susceptibility and stress response of different C. auris clades to antifungal drugs, thus opening a path to identifying novel clinical solutions counteracting the spread of these alarming pathogens.
Triglyceride and Triglyceride-Rich Lipoproteins in Atherosclerosis
Authors: Zhang Bai-Hui , Yin Fan , Qiao Ya-Nan , Guo Shou-Dong
Published: May 2022

Cardiovascular disease (CVD) is still the leading cause of death globally, and atherosclerosis is the main pathological basis of CVDs. Low-density lipoprotein cholesterol (LDL-C) is a strong causal factor of atherosclerosis. However, the first-line lipid-lowering drugs, statins, only reduce approximately 30% of the CVD risk. Of note, atherosclerotic CVD (ASCVD) cannot be eliminated in a great number of patients even their LDL-C levels meet the recommended clinical goals. Previously, whether the elevated plasma level of triglyceride is causally associated with ASCVD has been controversial. Recent genetic and epidemiological studies have demonstrated that triglyceride and triglyceride-rich lipoprotein (TGRL) are the main causal risk factors of the residual ASCVD. TGRLs and their metabolites can promote atherosclerosis via modulating inflammation, oxidative stress, and formation of foam cells. In this article, we will make a short review of TG and TGRL metabolism, display evidence of association between TG and ASCVD, summarize the atherogenic factors of TGRLs and their metabolites, and discuss the current findings and advances in TG-lowering therapies. This review provides information useful for the researchers in the field of CVD as well as for pharmacologists and clinicians.
Normative Data of the Trail Making Test Among Urban Community-Dwelling Older Adults in Japan
Authors: Suzuki Hiroyuki , Sakuma Naoko , Kobayashi Momoko , Ogawa Susumu , Inagaki Hiroki , Edahiro Ayako , Ura Chiaki , Sugiyama Mika , Miyamae Fumiko , Watanabe Yutaka , Shinkai Shoji , Awata Shuichi
Published: May 2022

Introduction

Population aging is likely to increase the number of people with dementia living in urban areas. The Trail Making Test (TMT) is widely used as a cognitive task to measure attention and executive function among older adults. Normative data from a sample of community-dwelling older adults are required to evaluate the executive function of this population. The purpose of this study was to examine the Trail Making Test completion rate and completion time among urban community-dwelling older adults in Japan.

Methods

A survey was conducted at a local venue or during a home visit (n = 1,966). Cognitive tests were conducted as a part of the survey, and TMT Parts A (TMT-A) and B (TMT-B) were completed after the completion of the Japanese version of the Mini-Mental State Examination (MMSE-J). Testers recorded TMT completion status, completion time, and the number of errors observed.

Results

In the TMT-A, 1,913 (99.5%) participants understood the instructions, and 1,904 (99.1%) participants completed the task within the time limit of 240 s. In the TMT-B, 1,839 (95.9%) participants understood the instructions, and 1,584 (82.6%) participants completed the task within the time limit of 300 s. The completion rate of TMT-B was 90.2 and 41.8% for participants with an MMSE-J score of >23 points and ≦23 points, respectively. Results of multiple regression analyses showed that age, education, and the MMSE-J score were associated with completion time in both TMTs.

Conclusion

In both TMTs, completion time was associated with age, education, and general cognitive function. However, not all participants completed the TMT-B, and the completion rate was relatively low among participants with low MMSE-J scores. These findings may help interpret future TMT assessments.
Perceptual Grouping During Binocular Rivalry in Mild Glaucoma
Authors: Issashar Leibovitzh Galia , Trope Graham E. , Buys Yvonne M. , Tarita-Nistor Luminita
Published: May 2022

Purpose

This study tested perceptual grouping during binocular rivalry to probe the strength of neural connectivity of the visual cortex involved in early visual processing in patients with mild glaucoma.

Methods

Seventeen patients with mild glaucoma with no significant visual field defects and 14 healthy controls participated. Rivalry stimuli were 1.8°-diameter discs, containing horizontal or vertical sine-wave gratings, viewed dichoptically. To test the grouping, two spatially separated identical stimuli were presented eccentrically to the same or different eyes and to the same or different hemifields. The outcome measures were the time of exclusive dominance of the grouped percept (i.e., percept with synchronized orientations), the rivalry rate, and the epochs of exclusive dominance.

Results

For both groups, the grouping occurred primarily for the matching orientations in the same eye/same hemifield (MO SE/SH) and for the matching orientations in the same eye/different hemifield (MO SE/DH) conditions. Time dominance of the grouped percept of the glaucoma group was similar to that of the control group in all conditions. The rivalry rates in the MO SE/SH and MO SE/DH conditions were significantly larger in the control group than in the glaucoma group. The epochs of exclusive dominance of the grouped percept in the MO SE/SH condition were a median of 48-ms longer for the control group, but a median of 116-ms shorter for the glaucoma group when compared to those in the MO SE/DH condition.

Conclusion

Patients with mild glaucoma show clear impairments in binocular rivalry while evidence for deficits in perceptual grouping could be inferred only indirectly. If these deficits truly exist, they may have implications for higher levels of visual processing, such as object recognition and scene segmentation, but these predictions remain to be tested in future studies.
Differential Abnormality in Functional Connectivity Density in Preclinical and Early-Stage Alzheimer's Disease
Authors: Song Yu , Wu Huimin , Chen Shanshan , Ge Honglin , Yan Zheng , Xue Chen , Qi Wenzhang , Yuan Qianqian , Liang Xuhong , Lin Xingjian , Chen Jiu
Published: May 2022

Background

Both subjective cognitive decline (SCD) and amnestic mild cognitive impairment (aMCI) have a high risk of progression to Alzheimer's disease (AD). While most of the available evidence described changes in functional connectivity (FC) in SCD and aMCI, there was no confirmation of changes in functional connectivity density (FCD) that have not been confirmed. Therefore, the purpose of this study was to investigate the specific alterations in resting-state FCD in SCD and aMCI and further assess the extent to which these changes can distinguish the preclinical and early-stage AD.

Methods

A total of 57 patients with SCD, 59 patients with aMCI, and 78 healthy controls (HC) were included. The global FCD, local FCD, and long-range FCD were calculated for each voxel to identify brain regions with significant FCD alterations. The brain regions with abnormal FCD were then used as regions of interest for FC analysis. In addition, we calculated correlations between neuroimaging alterations and cognitive function and performed receiver-operating characteristic analyses to assess the diagnostic effect of the FCD and FC alterations on SCD and aMCI.

Results

FCD mapping revealed significantly increased global FCD in the left parahippocampal gyrus (PHG.L) and increased long-range FCD in the left hippocampus for patients with SCD when compared to HCs. However, when compared to SCD, patients with aMCI showed significantly decreased global FCD and long-range FCD in the PHG.L. The follow-up FC analysis further revealed significant variations between the PHG.L and the occipital lobe in patients with SCD and aMCI. In addition, patients with SCD also presented significant changes in FC between the left hippocampus, the left cerebellum anterior lobe, and the inferior temporal gyrus. Moreover, changes in abnormal indicators in the SCD and aMCI groups were significantly associated with cognitive function. Finally, combining FCD and FC abnormalities allowed for a more precise differentiation of the clinical stages.

Conclusion

To our knowledge, this study is the first to investigate specific alterations in FCD and FC for both patients with SCD and aMCI and confirms differential abnormalities that can serve as potential imaging markers for preclinical and early-stage Alzheimer's disease (AD). Also, it adds a new dimension of understanding to the diagnosis of SCD and aMCI as well as the evaluation of disease progression.
Gene Expression Profiles of the Aging Rat Hippocampus Imply Altered Immunoglobulin Dynamics
Authors: Giannos Panagiotis , Prokopidis Konstantinos
Published: May 2022

Aging is a process that leads to the deterioration in physiological functioning of the brain. Prior research has proposed that hippocampal aging is accompanied by genetic alterations in neural, synaptic, and immune functions. Nevertheless, interactome-based interrogations of gene alterations in hippocampal aging, remain scarce. Our study integrated gene expression profiles of the hippocampus from young and aged rats and functionally classified network-mapped genes based on their interactome. Hippocampal differentially expressed genes (DEGs) between young (5–8 months) and aged (21–26 months) male rats (Rattus norvegicus) were retrieved from five publicly available datasets (GSE14505, GSE20219, GSE14723, GSE14724, and GSE14725; 38 young and 29 aged samples). Encoded hippocampal proteins of age-related DEGs and their interactome were predicted. Clustered network DEGs were identified and the highest-ranked was functionally annotated. A single cluster of 19 age-related hippocampal DEGs was revealed, which was linked with immune response (biological process, P = 1.71E-17), immunoglobulin G binding (molecular function, P = 1.92E-08), and intrinsic component of plasma membrane (cellular component, P = 1.25E-06). Our findings revealed dysregulated hippocampal immunoglobulin dynamics in the aging rat brain. Whether a consequence of neurovascular perturbations and dysregulated blood-brain barrier permeability, the role of hippocampal immunoregulation in the pathobiology of aging warrants further investigation.
Mastery Is Associated With Weight Status, Food Intake, Snacking, and Eating Disorder Symptoms in the NutriNet-Santé Cohort Study
Authors: Gisch Ulrike A. , Robert Margaux , Berlin Noémi , Nebout Antoine , Etilé Fabrice , Teyssier Sabrina , Andreeva Valentina A. , Hercberg Serge , Touvier Mathilde , Péneau Sandrine
Published: May 2022

Mastery is a psychological resource that is defined as the extent to which individuals perceive having control over important circumstances of their lives. Although mastery has been associated with various physical and psychological health outcomes, studies assessing its relationship with weight status and dietary behavior are lacking. The aim of this cross-sectional study was to assess the relationship between mastery and weight status, food intake, snacking, and eating disorder (ED) symptoms in the NutriNet-Santé cohort study. Mastery was measured with the Pearlin Mastery Scale (PMS) in 32,588 adults (77.45% female), the mean age was 50.04 (14.53) years. Height and weight were self-reported. Overall diet quality and food group consumption were evaluated with ≥3 self-reported 24-h dietary records (range: 3–27). Snacking was assessed with an ad-hoc question. ED symptoms were assessed with the Sick-Control-One-Fat-Food Questionnaire (SCOFF). Linear and logistic regression analyses were conducted to assess the relationship between mastery and weight status, food intake, snacking, and ED symptoms, controlling for sociodemographic and lifestyle characteristics. Females with a higher level of mastery were less likely to be underweight (OR: 0.88; 95%CI: 0.84, 0.93), overweight [OR: 0.94 (0.91, 0.97)], or obese [class I: OR: 0.86 (0.82, 0.90); class II: OR: 0.76 (0.71, 0.82); class III: OR: 0.77 (0.69, 0.86)]. Males with a higher level of mastery were less likely to be obese [class III: OR: 0.75 (0.57, 0.99)]. Mastery was associated with better diet quality overall, a higher consumption of fruit and vegetables, seafood, wholegrain foods, legumes, non-salted oleaginous fruits, and alcoholic beverages and with a lower consumption of meat and poultry, dairy products, sugary and fatty products, milk-based desserts, and sweetened beverages. Mastery was also associated with lower snacking frequency [OR: 0.89 (0.86, 0.91)] and less ED symptoms [OR: 0.73 (0.71, 0.75)]. As mastery was associated with favorable dietary behavior and weight status, targeting mastery might be a promising approach in promoting healthy behaviors.

Clinical Trial Registry Number

NCT03335644 at Clinicaltrials.gov.
A Systematic Review of the Methods Used to Assess and Report Dietary Patterns
Authors: Wingrove Kate , Lawrence Mark A. , McNaughton Sarah A.
Published: May 2022

The use of dietary pattern assessment methods has increased over time. However, data from individual studies can be difficult to compare and synthesize when the dietary pattern assessment methods, and the dietary patterns that are identified are not described sufficiently. The aims of this systematic review were to analyze the application and reporting of dietary pattern assessment methods, and the reporting of the dietary patterns that were analyzed with health outcomes. Three electronic databases were searched (Medline, Embase, and Global Health). Cohort and nested case control studies published in English between January 1980 and March 2019 that examined associations between dietary patterns and health outcomes (including cardiovascular disease, cancer, diabetes and metabolic syndrome, and body weight) in apparently healthy, community dwelling adults (aged over 18 years) or children (aged 2–18 years) were eligible for inclusion. A narrative synthesis was conducted and descriptive statistics were used to summarize the application and reporting of each dietary pattern assessment method, and the reporting of the identified dietary patterns. Analysis of associations between dietary patterns and health outcomes was beyond the scope of this review. Of the included studies (n = 410), 62.7% used index-based methods, 30.5% used factor analysis or principal component analysis, 6.3% used reduced rank regression, and 5.6% used cluster analysis. Some studies (4.6%) used more than one method. There was considerable variation in the application and reporting of dietary pattern assessment methods. For example, the application of Mediterranean diet indices varied in terms of the nature of dietary components (foods only or foods and nutrients) and the rationale behind the cut-off points (absolute and/or data driven). In some cases, important methodological details were omitted. The level of detail used to describe the dietary patterns also varied, and food and nutrient profiles were often not reported. To ensure dietary patterns evidence can be synthesized and translated into dietary guidelines, standardized approaches for the application and reporting of dietary pattern assessment methods and the reporting of the identified dietary patterns would be beneficial.
The Rate of Histamine Degradation by Diamine Oxidase Is Compromised by Other Biogenic Amines
Authors: Sánchez-Pérez Sònia , Comas-Basté Oriol , Costa-Catala Judit , Iduriaga-Platero Irache , Veciana-Nogués M. Teresa , Vidal-Carou M. Carmen , Latorre-Moratalla M. Luz
Published: May 2022

Nowadays, certain uncertainties related to the onset of histamine adverse effects remain unsolved and still require further research. Questions still to be resolved include the wide range of doses at which dietary histamine may trigger symptoms of intoxication (100–10,000 mg/kg) or the appearance of symptoms of histamine intolerance after the consumption of foods presumable without histamine. It seems feasible that other amines, by acting as competitive substrates, could interfere with histamine degradation by the intestinal enzyme diamine oxidase (DAO). Therefore, the aim of this study was to elucidate the interference of different amines on the rate of histamine degradation by DAO. A series of in vitro enzymatic assays were performed using histamine as the reaction substrate combined with different proportions of putrescine, cadaverine, tyramine, spermidine, and spermine (1:0.25, 1:1, 1:4, 1:20). Putrescine and cadaverine significantly delayed histamine degradation at all tested concentrations (p < 0.001). The greatest effect was observed when putrescine or cadaverine concentrations were 20-fold higher than that of histamine, its degradation being reduced by 70 and 80%, respectively, compared to histamine alone (28.16 ± 1.0 mU). In contrast, tyramine, spermidine and spermine significantly inhibited the histamine degradation rate only at the highest concentration (1:20), reducing it by 32–45%. These results demonstrate that other biogenic amines interfere with histamine metabolization by DAO in vitro, the extent depending on the substrate. These findings could explain why susceptibility to dietary histamine is so variable and account for the discrepancies in the scientific databases regarding the amount of histamine that triggers adverse health effects.
FAPI-PET/CT in Cancer Imaging: A Potential Novel Molecule of the Century
Authors: Huang Rong , Pu Yu , Huang Shun , Yang Conghui , Yang Fake , Pu Yongzhu , Li Jindan , Chen Long , Huang Yunchao
Published: May 2022

Fibroblast activation protein (FAP), a type II transmembrane serine protease, is highly expressed in more than 90% of epithelial tumors and is closely associated with various tumor invasion, metastasis, and prognosis. Using FAP as a target, various FAP inhibitors (FAPIs) have been developed, most of which have nanomolar levels of FAP affinity and high selectivity and are used for positron emission tomography (PET) imaging of different tumors. We have conducted a systematic review of the available data; summarized the biological principles of FAPIs for PET imaging, the synthesis model, and metabolic characteristics of the radiotracer; and compared the respective values of FAPIs and the current mainstream tracer 18F-Fludeoxyglucose (18F-FDG) in the clinical management of tumor and non-tumor lesions. Available research evidence indicates that FAPIs are a molecular imaging tool complementary to 18F-FDG and are expected to be the new molecule of the century with better imaging effects than 18F-FDG in a variety of cancers, including gastrointestinal tumors, liver tumors, breast tumors, and nasopharyngeal carcinoma.
Case Report: GNAQ- and SF3B1 Mutations in an Aggressive Case of Relapsing Uveal Ring Melanoma
Authors: Prasuhn Michelle , Freitag Josephine Christin , Lüken Sabine , Kakkassery Vinodh , Merz Hartmut , Caliebe Almuth , Spielmann Malte , Ranjbar Mahdy , Rommel Felix
Published: May 2022

The molecular mechanisms for uveal ring melanoma are still unclear until today. In this case report, we describe a patient with a malignant uveal melanoma with exudative retinal detachment that had been treated with plaque brachytherapy, resulting in successful tumor regression. After 1 year, a ring-shaped recurrence with extraocular extension appeared, and the eye required enucleation. Histological and molecular genetic analyses revealed an epithelioid-cell-type melanoma with complete circumferential involvement of the ciliary body and, so far, unreported GNAQ and SF3B1 mutations in ring melanoma. Therefore, this report gives new genetic background information on this ocular tumor usually leading to enucleation.
Extracellular Vesicles and Hepatocellular Carcinoma: Opportunities and Challenges
Authors: Wang Juan , Wang Xiaoya , Zhang Xintong , Shao Tingting , Luo Yanmei , Wang Wei , Han Yunwei
Published: May 2022

The incidence of hepatocellular carcinoma (HCC) is increasing worldwide. Extracellular vesicles (EVs) contain sufficient bioactive substances and are carriers of intercellular information exchange, as well as delivery vehicles for nucleic acids, proteins and drugs. Although EVs show great potential for the treatment of HCC and their role in HCC progression has been extensively studied, there are still many challenges such as time-consuming extraction, difficult storage, easy contamination, and low drug loading rate. We focus on the biogenesis, morphological characteristics, isolation and extraction of EVs and their significance in the progression of HCC, tumor invasion, immune escape and cancer therapy for a review. EVs may be effective biomarkers for molecular diagnosis of HCC and new targets for tumor-targeted therapy.