bluuebunny/crossref_metadata_2025 huggingface dataset:
Horizontal Transfer of miR-643 from Cisplatin-Resistant Cells Confers Chemoresistance to Recipient Drug-Sensitive Cells by Targeting APOL6
Authors: Raji Grace R. , Poyyakkara Aswini , Krishnan Anjali Kunhi , Maurya Ashutosh Kumar , Changmai Udeshna , Shankar Sharath S. , Kumar V. B. Sameer
Published: May 2021

Acquisition of resistance to cisplatin is a major impediment to the success of cisplatin-based combination therapies for cancer. Recent studies indicate that exosomal miRNAs derived from drug-resistant tumour cells can confer resistance properties to recipient cells by a horizontal transfer mechanism. Although the role of horizontal transfer of a few miRNAs has been described, little is known about the concerted action of horizontal transfer of miRNAs in conferring cisplatin resistance. The present study was designed to identify the role of miR-643, which is one of the most significantly increased miRNA in exosomes released from cisplatin-resistant Heptocarcinoma cells, in altering the cisplatin resistance properties of recipient cells. Drug-sensitivity assays involving miR-643 revealed that ectopic expression of miR-643 can desensitise the cells towards cisplatin. Furthermore, we identified APOL6 as a major target of miR-643. Further mechanistic studies showed that miR-643 can modulate APOL6 mRNA and protein levels, leading to a reversal of APOL6-mediated apoptosis. Altogether, our results suggest an APOL6-dependent mechanism for miR-643 mediated cisplatin resistance upon the horizontal transfer across cell types.
Utilizing Developmentally Essential Secreted Peptides Such as Thymosin Beta-4 to Remind the Adult Organs of Their Embryonic State—New Directions in Anti-Aging Regenerative Therapies
Authors: Maar Klaudia , Hetenyi Roland , Maar Szabolcs , Faskerti Gabor , Hanna Daniel , Lippai Balint , Takatsy Aniko , Bock-Marquette Ildiko
Published: May 2021

Our dream of defeating the processes of aging has occupied the curious and has challenged scientists globally for hundreds of years. The history is long, and sadly, the solution is still elusive. Our endeavors to reverse the magnitude of damaging cellular and molecular alterations resulted in only a few, yet significant advancements. Furthermore, as our lifespan increases, physicians are facing more mind-bending questions in their routine practice than ever before. Although the ultimate goal is to successfully treat the body as a whole, steps towards regenerating individual organs are even considered significant. As our initial approach to enhance the endogenous restorative capacity by delivering exogenous progenitor cells appears limited, we propose, utilizing small molecules critical during embryonic development may prove to be a powerful tool to increase regeneration and to reverse the processes associated with aging. In this review, we introduce Thymosin beta-4, a 43aa secreted peptide fulfilling our hopes and capable of numerous regenerative achievements via systemic administration in the heart. Observing the broad capacity of this small, secreted peptide, we believe it is not the only molecule which nature conceals to our benefit. Hence, the discovery and postnatal administration of developmentally relevant agents along with other approaches may result in reversing the aging process.
Study of the Performance of Particles Based on Modified Starches Containing Potassium Sorbate and Incorporated into Biodegradable Films: Physicochemical Characterization and Antimicrobial Action
Authors: Alzate Paola , Gerschenson Lía , Flores Silvia
Published: May 2021

Ultrasound technique was used to produce native and acetylated cassava starch particles containing potassium sorbate (KS). In order to obtain an active packaging, films with addition of native starch particles containing KS (NKSPF) or added with acetylated starch particles containing KS (AKSPF) were formulated. As control systems, films without KS (CF) or added with KS that was not retained in particles (KSF), were produced. The NKSPF and AKSPF microstructure was consistent with composite materials. Tensile test revealed that CF and KSF were ductile and extensible (stress at break (σb) 2.8–2.5 MPa and strain at break (εb) 284–206%), while NKSPF and AKSPF were more resistant films with higher Young’s Modulus (148–477 MPa) and σb (3.6–17 MPa) but lower εb (40–11%). Moreover, NKSPF and AKSPF developed lower Yellowness Index (6.6–6.5) but higher opacity (19–23%) and solubility in water (31–35%) than KSF (9, 10.8% and 9%, respectively). It was observed that KSF and NKSPF moderately reduced the Zygosaccharomyces bailii growth while AKSPF showed the highest yeast inhibition, three Log-cycles, compared to CF. Additionally, FTIR spectroscopy revealed intensified interactions between KS and modified starch. It was concluded that starch sonication and acetylation were useful modifications to produce particles carrier of KS that improved the physical and antimicrobial performance of active films.
Anthropometric Development in Children: Possible Changes in Body Mass, Basal Metabolic Rate and Inflammatory Status
Authors: Martin-Hadmaș Roxana Maria , Martin Ștefan Adrian , Romonți Adela , Mărginean Cristina Oana
Published: May 2021

(1) Background: Worldwide, public health policies focus on studying dietary patterns and the related anthropometric changes in children. Their aim is to improve the measures meant to reduce global malnutrition rates. Our goal was to study the main changes in the inflammatory status related to anthropometric changes and total daily energy intake. (2) Methods: We tested the study hypothesis by analyzing serum IL-6 and IL-8 levels, cholesterol and triglycerides values, as well as total proteins and creatinine levels, RMR, and food journals in a sample of 160 healthy subjects aged between 6 and 12 years old. (3) Results: IL-6 was correlated with the skinfold values. Changes in the skinfolds were significantly correlated with total proteins and triglycerides. Both weight for age and height for age were related to the skinfold values. Through the BMR measurements, peak carbohydrate metabolism changed significantly based upon IL-6 values, which were significantly correlated with the respiratory coefficient values. Based on the basal metabolic rate, an increased IL-8 ratio was related to the RQ value. (4) Conclusions: Skinfolds have been significantly correlated with IL-6 and IL-8 levels. With changes in body weight, we encountered differences in both serum cholesterol and serum triglycerides values, unlike total proteins and creatinine, which failed to change.
Innovative Coatings of Metallic Alloys Used as Bioactive Surfaces in Implantology: A Review
Authors: Prodana Mariana , Stoian Andrei Bogdan , Burnei Cristian , Ionita Daniela
Published: May 2021

Metallic implants are widely used in the field of implantology, but there are still problems leading to implant failures due to weak osseointegration, low mechanical strength for the implant, inadequate antibacterial properties, and low patient satisfaction. Implant failure can be caused by bacterial infections and poor osteointegration. To improve the implant functionalization, many researchers focus on surface modifications to prepare the proper physical and chemical conditions able to increase biocompatibility and osteointegration between implant and bone. Improving the antibacterial performance is also a key factor to avoid the inflammation in the human body. This paper is a brief review for the types of coatings used to increase osseointegration and biocompatibility for the successful use of metal alloys in the field of implantology.
Analysis of the Resistance to Bending of Gypsum with Added Graphene
Authors: Jara Luis M. Serna , Yepes José A. Flores , Pérez Joaquín J. Pastor
Published: May 2021

Gypsum-based interior coatings are used due to their excellent fire resistance and thermal and acoustic properties. In recent decades, the development of these calcium sulfate-based coatings has been linked with the use of new additives that allow the setting times and the water content of the mix to be controlled and offer the possibility of adding different aggregates to the mortar, which further improve its resistance properties. In this article, significant results are presented that compare the flexural strength of mortars formed using added construction plaster with different percentages of graphene powder.
Structural and Electric Properties of Epitaxial Na0.5Bi0.5TiO3-Based Thin Films
Authors: Magalhaes Bruno , Engelhardt Stefan , Molin Christian , Gebhardt Sylvia E. , Nielsch Kornelius , Hühne Ruben
Published: May 2021

Substantial efforts are dedicated worldwide to use lead-free materials for environmentally friendly processes in electrocaloric cooling. Whereas investigations on bulk materials showed that Na0.5Bi0.5TiO3 (NBT)-based compounds might be suitable for such applications, our aim is to clarify the feasibility of epitaxial NBT-based thin films for more detailed investigations on the correlation between the composition, microstructure, and functional properties. Therefore, NBT-based thin films were grown by pulsed laser deposition on different single crystalline substrates using a thin epitaxial La0.5Sr0.5CoO3 layer as the bottom electrode for subsequent electric measurements. Structural characterization revealed an undisturbed epitaxial growth of NBT on lattice-matching substrates with a columnar microstructure, but high roughness and increasing grain size with larger film thickness. Dielectric measurements indicate a shift of the phase transition to lower temperatures compared to bulk samples as well as a reduced permittivity and increased losses at higher temperatures. Whereas polarization loops taken at −100 °C revealed a distinct ferroelectric behavior, room temperature data showed a significant resistive contribution in these measurements. Leakage current studies confirmed a non-negligible conductivity between the electrodes, thus preventing an indirect characterization of the electrocaloric properties of these films.
Structural and Magnetic Properties of Potassium-Doped 2,3-DiMethylnaphthalene
Authors: Wu Xiao-Lin , Wang Ren-Shu , Yang Hui , Zhang Jie , Fu Ming-An , Fang Shi-Chao , Chen Xiao-Jia , Gao Yun , Huang Zhong-Bing
Published: May 2021

The development of potential magnetic materials in metal-doped polycyclic aromatic hydrocarbons has been a research hotspot in recent years. Here we have successfully synthesized stable potassium-doped 2,3-dimethylnaphthalene samples. The combination of first-principles calculations and XRD results identifies that doping of potassium into 2,3-dimethylnaphthalene forms a monoclinic structure with a molar ratio of 1:2 between potassium and molecule. The red shifts in the Raman spectra indicate that potassium 4s electrons are transferred to the organic molecules. The magnetic measurements show that the doped materials exhibit a temperature-independent magnetization in the temperature region of 1.8–300 K, which is consistent with the Pauli paramagnetic behavior. This is distinct from the diamagnetism of pristine material. Compared to the previous focus on benzene ring structure, our study of aromatic hydrocarbon derivatives of benzene ring opens a new route for the development of this field.
A Computational Validation of Water Molecules Adsorption on an NaCl Surface
Authors: Liu Xiao-Yan , Cao Jing-Wen , Qin Xiao-Ling , Zhu Xu-Liang , Yu Xu-Hao , Wang Xue-Chun , Yuan Xiao-Qing , Liu Yu-He , Wang Yong , Zhang Peng
Published: May 2021

It was reported that a scanning tunneling microscopy (STM) study observed the adsorption geometry of a water monomer and a tetramer on NaCl(100) film. Based on first-principles density functional theory (DFT), the adsorption behavior of water on the NaCl surface was simulated with CASTEP code. The results showed that the water monomer almost lay on the NaCl(001) surface with one O–H bond tilted slightly downward. This was quite different from the STM observations. In fact, the experimental observation was influenced by the Au(111) substrate, which showed an upright form. A recent report on observations of two-dimensional ice structure on Au(111) substrate verified our simulations. However, the water tetramer formed a stable quadrate structure on the surface, which was consistent with observation. The intermolecular hydrogen bonds present more strength than surface adsorption. The simulations presented a clearer picture than experimental observations.
Structure and Properties of Ln2MoO6 Oxymolybdates (Ln = La, Pr, Nd) Doped with Magnesium
Authors: Orlova Ekaterina , Kharitonova Elena , Sorokin Timofei , Antipin Alexander , Novikova Nataliya , Sorokina Nataliya , Voronkova Valentina
Published: May 2021

The literature data and the results obtained by the authors on the study of the structure and properties of a series of polycrystalline and single-crystal samples of pure and Mg-doped oxymolybdates Ln2MoO6 (Ln = La, Pr, Nd) are analyzed. Presumably, the high-temperature phase I41/acd of Nd2MoO6 single crystals is retained at room temperature. The reason for the loss of the center of symmetry in the structures of La2MoO6 and Pr2MoO6 and the transition to the space group I4¯c2 is the displacement of oxygen atoms along the twofold diagonal axes. In all structures, Mg cations are localized near the positions of the Mo atoms, and the splitting of the positions of the atoms of rare-earth elements is found. Thermogravimetric studies, as well as infrared spectroscopy data for hydrated samples of Ln2MoO6 (Ln = La, Pr, Nd), pure and with an impurity of Mg, confirm their hygroscopic properties.
Can the MMPI Predict Adult ADHD? An Approach Using Machine Learning Methods
Authors: Kim Sunhae , Lee Hye-Kyung , Lee Kounseok
Published: May 2021

(1) Background: Adult attention-deficit/hyperactivity disorder (ADHD) symptoms cause various social difficulties due to attention deficit and impulsivity. In addition, in contrast to ADHD in childhood, ADHD in adulthood is difficult to diagnose due to mixed psychopathologies. This study aimed to determine whether it is possible to predict ADHD symptoms in adults using the Minnesota Multiphasic Personality Inventory-2 (MMPI-2) with machine learning (ML) techniques; (2) Methods: Data collected from 5726 college students were analyzed. The MMPI-2-Restructured Form (MMPI-2-RF) was used, and ADHD symptoms in adults were evaluated using the Attention-Deficit/Hyperactivity Disorder Self-Report Scale (ASRS). For statistical analysis, three ML algorithms were used, i.e., K-nearest neighbors (KNN), linear discriminant analysis (LDA), and random forest, with the ASRS evaluation result as the dependent variable and the 50 MMPI-2-RF scales as predictors; (3) Results: When the KNN, LDA, and random forest techniques were applied, the accuracy was 93.1%, 91.2%, and 93.6%, respectively, and the area under the curve (AUC) was 0.722, 0.806, and 0.790, respectively. The AUC of the LDA method was the largest, with an excellent level of diagnostic accuracy; (4) Conclusions: ML using the MMPI-2 in a large group could provide reliable accuracy in screening for adult ADHD.
Enhanced Sensing Behavior of Three-Dimensional Microfluidic Paper-Based Analytical Devices (3D-μPADs) with Evaporation-Free Enclosed Channels for Point-of-Care Testing
Authors: Jeon Jaehyung , Park Chanyong , Ponnuvelu Dinesh Veeran , Park Sungsu
Published: May 2021

Despite the potential in fabrication of microfluidic paper-based analytical devices (μPADs) for point-of-care testing (POCT) kits, the development of simple, accurate, and rapid devices with higher sensitivity remains challenging. Here, we report a novel method for 3D-μPAD fabrication with enclosed channels using vat photopolymerization to avoid fluid evaporation. In detail, height of the enclosed channels was adjusted from 0.3 to 0.17 mm by varying the UV exposure time from 1 to 4 s for the top barrier, whereas the exposure time for the bottom and side barriers was fixed. As a result, sample flow in the enclosed channels of 3D-μPADs showed lesser wicking speed with very scant evaporation compared to that in the hemi channels in the 3D-μPADs. The stoppage of evaporation in the enclosed channels significantly improved the gray intensity and uniformity in the detection zone of the 3D-μPADs, resulting in as low as 0.3 mM glucose detection. Thus 3D-μPADs with enclosed channels showed enhanced sensitivity compared to the 3D-μPADs with hemi channels when dealing with a small volume sample. Our work provides a new insight into 3D-μPAD design with enclosed channels, which redefines the methodology in 3D printing.
Impact of FGFR4 Gene Polymorphism on the Progression of Colorectal Cancer
Authors: Shiu Bei-Hao , Hsieh Ming-Hong , Ting Wen-Chien , Chou Ming-Chih , Chang Lun-Ching , Huang Chi-Chou , Su Shih-Chi , Yang Shun-Fa
Published: May 2021

Colorectal cancer (CRC) is a multifactorial malignancy, and its high incidence and mortality rate remain a global public health burden. Fibroblast growth factor receptor 4 (FGFR4) is a receptor tyrosine kinase that has been shown to play a key role in cancer development and prognosis via the activation of its downstream oncogenic signaling pathways. The present study aimed to explore the impact of FGFR4 gene polymorphisms on the risk and progression of CRC. Three FGFR4 single-nucleotide polymorphisms (SNPs), including rs1966265, rs351855, and rs7708357, were evaluated in 413 CRC cases and 413 gender- and age-matched cancer-free controls. We did not observe any significant association of three individual SNPs with the risk of CRC between the case and control group. However, while assessing the clinicopathological parameters, patients of rectal cancer possessing at least one minor allele of rs1966265 (AG and GG; AOR, 0.236; p = 0.046) or rs351855 (GA and AA; AOR, 0.191; p = 0.022) were found to develop less metastasis as compared to those who are homozygous for the major allele. Further analyses using the datasets from the Genotype-Tissue Expression (GTEx) Portal and The Cancer Genome Atlas (TCGA) revealed that rs351855 regulated FGFR4 expression in many human tissues, and increased FGFR4 levels were associated with the occurrence, advanced stage, and distal metastasis of colon adenocarcinoma. These data suggest that the amino acid change in combination with altered expression levels of FGFR4 due to genetic polymorphisms may affect CRC progression.
A CT-Based Radiomic Signature Can Be Prognostic for 10-Months Overall Survival in Metastatic Tumors Treated with Nivolumab: An Exploratory Study
Authors: Corino Valentina D. A. , Bologna Marco , Calareso Giuseppina , Licitra Lisa , Ghi Mariagrazia , Rinaldi Gaetana , Caponigro Francesco , Morelli Franco , Airoldi Mario , Allegrini Giacomo , Cassano Alessandra , Ferrari Daris , Mirabile Aurora , Tosoni Alicia , Galizia Danilo , Merlano Marco , Sponghini Andrea , Moretti Gabriella , Mainardi Luca , Bossi Paolo
Published: May 2021

Baseline clinical prognostic factors for recurrent and/or metastatic (RM) head and neck squamous cell carcinoma (HNSCC) treated with immunotherapy are lacking. CT-based radiomics may provide additional prognostic information. A total of 85 patients with RM-HNSCC were enrolled for this study. For each tumor, radiomic features were extracted from the segmentation of the largest tumor mass. A pipeline including different feature selection steps was used to train a radiomic signature prognostic for 10-month overall survival (OS). Features were selected based on their stability to geometrical transformation of the segmentation (intraclass correlation coefficient, ICC > 0.75) and their predictive power (area under the curve, AUC > 0.7). The predictive model was developed using the least absolute shrinkage and selection operator (LASSO) in combination with the support vector machine. The model was developed based on the first 68 enrolled patients and tested on the last 17 patients. Classification performance of the radiomic risk was evaluated accuracy and the AUC. The same metrics were computed for some baseline predictors used in clinical practice (volume of largest lesion, total tumor volume, number of tumor lesions, number of affected organs, performance status). The AUC in the test set was 0.67, while accuracy was 0.82. The performance of the radiomic score was higher than the one obtainable with the clinical variables (largest lesion volume: accuracy 0.59, AUC = 0.55; number of tumoral lesions: accuracy 0.71, AUC 0.36; number of affected organs: accuracy 0.47; AUC 0.42; total tumor volume: accuracy 0.59, AUC 0.53; performance status: accuracy 0.41, AUC = 0.47). Radiomics may provide additional baseline prognostic value compared to the variables used in clinical practice.
Non-Classical Congenital Adrenal Hyperplasia-Causing Alleles in Adolescent Girls with PCOS and in Risk Group for PCOS Development
Authors: Lidaka Lasma , Bekere Laine , Lazdane Gunta , Dzivite-Krisane Iveta , Kivite-Urtane Anda , Gailite Linda
Published: May 2021

Background: Polycystic ovary syndrome (PCOS) is the most common endocrinopathy in women. Depending on the diagnostic criteria applied, it occurs in up to 16.6% of the general female population. Congenital adrenal hyperplasia includes a group of autosomal recessive disorders, the most common of which is non-classical congenital adrenal hyperplasia (NCAH) caused by mutations in the CYP21A2 gene. PCOS and NCAH have similar clinical manifestations (hyperandrogenemia, i.e., hirsutism, acne, alopecia, and increased androgen levels in the blood) and potential impact on long-term health (infertility, increased risk of type 2 diabetes, and cardiovascular disease. Consequently, it is thought that NCAH mutations in the heterozygous state may play a role in PCOS development and phenotypic expression. Objective: To determine the prevalence of the most common pathogenic alleles of the CYP21A2 gene in adolescents with PCOS and adolescents at risk of PCOS development, and to compare the results with healthy adolescents matched for gynecological age. Methods: A cross-sectional study was conducted with 55 PCOS patients, 23 risk patients (with hyperandrogenism but a normal menstrual cycle), and 49 healthy adolescents. Genetic variations in the CYP21A2 gene were analyzed using a standard Multiplex Ligation-dependent Probe Amplification test (SALSA MLPA Probemix P050-C1 CAH; MRC Holland). Results: No significant differences were found among the three groups regarding the frequency of carriers of NCAH variations in the heterozygous state. It was found that the I172N carrier in the PCOS group had a significantly higher Global Acne Grading Scale score than PCOS patients without this variation (p = 0.038). Within the control group of healthy adolescents, compound heterozygous carriers (IVS2-12A > G and -113G > A) had a significantly higher body mass index than non-carriers (p = 0.036). Conclusion: We found no differences in the incidence of NCAH-causing variations in the heterozygous state in adolescent PCOS patients, risk adolescents (with hirsutism but normal menstruation), and healthy adolescents. Future studies of larger cohorts and rarer pathogenic CYP21A2 gene variations are required.
Infrared Thermography as a Method of Verification in Raynaud’s Phenomenon
Authors: Lindberg Lotte , Kristensen Bent , Eldrup Ebbe , Thomsen Jane Frølund , Jensen Lars Thorbjørn
Published: May 2021

Raynaud’s phenomenon (RP) is characterized by the episodic whitening of the fingers upon exposure to cold. A recently described thermographic algorithm was proposed as a diagnostic replacement of the currently applied finger systolic pressure (FSP) test. The aim of the study was to evaluate the performance of the thermographic algorithm when applied in patients suspected of having RP. Forty-three patients were examined using thermographic imaging after local cooling of the hands in water of 10 °C for 1 min. The thermographic algorithm was applied to predict the probability of RP. The performance of the algorithm was evaluated with different cut-off levels. A new algorithm was proposed based on patients from the target population. The performance of the tested algorithm was noninferior to the FSP test, when a cut-off level of 0.05 was applied, yielding a sensitivity and specificity of 69% and 58%, respectively. The accuracy was 66%. The FSP test had a sensitivity and specificity of 77% and 37%, respectively, and the accuracy was 59%. The thermographic method proved useful for detecting RP and was able to replace the FSP test as a diagnostic test. The alternative algorithm revealed that other thermographic variables were more predictive of the target population, but this should be verified in future patients.
Survival of Single-Unit Porcelain-Fused-to-Metal (PFM) and Metal Crowns Placed by Students at an Australian University Dental Clinic over a Five-Year Period
Authors: Carey Chris , Din Nick Del , Lamb Jessica , Wright Hazel , Robb Nigel D. , Abuzar Menaka
Published: May 2021

The aim of this retrospective study was to determine the survival rate of single-unit porcelain-fused-to-metal (PFM) and metal crowns placed by dental students at an Australian university undergraduate dental clinic over a five-year period. Complications and the incidences of crown failures were recorded. Clinical records pertaining to single-unit PFM and metal crowns inserted over a five-year period were reviewed, including patient-related, tooth-related, and procedural factors for each crown. Crowns were evaluated as surviving, surviving with complications, or failed. Kaplan–Meier statistical analysis was used to estimate survival rate., This study is based on a sample of 232 (78.4%) PFM crowns and 64 (21.6%) metal crowns inserted between 2014 and 2018. Cumulatively, 224 (75.7%) were surviving, 48 (16.2%) were surviving but previously had complications, and 24 (8.1%) failed. The 5-year cumulative survival rate of all PFM and metal crowns was 83.9% (0.839 ± 0.038, Kaplan–Meier). The average survival time for all crowns was 4.432 ± 0.089 years. Comparatively, PFM crowns had a higher survival rate at 1 year (0.972 ± 0.010) and 2 years (0.919 ± 0.017), compared to metal crowns at 1 year (0.964 ± 0.011) and 2 years (0.894± 0.018). The survival rate of metal crowns remained constant from 2 years to 4 years and thereafter, whereas there was a continued decline in the survival rate of PFM crowns to 83.2% (0.832 ± 0.038) at 4 years and thereafter. Crowns placed on premolars had the highest cumulative survival rate whereas those placed on molars exhibited the lowest survival rate for the duration of the study period. Despite single-unit PFM crowns having a higher 1- and 2-year survival rate compared to metal crowns, metal crowns had a higher survival rate at 4 years and thereafter. Survival rates are comparable to previous studies.
Trap-Related Reliability Problems of Dielectrics in Memory Cells
Authors: Watanabe Hiroshi , Lin Hsin-Jyun
Published: May 2021

A basic mechanism for storing data in memory cells is to record changes in electronic charges, material phases, resistivities, magnetic properties, and so forth. The change in electronic charge has been widely used in the majority of mass-produced memories, such as dynamic random-access memory (DRAM), static random-access memory (SRAM), NOR Flash, and NAND Flash. Other emerging memories have collected widespread attention for acquiring extra advantages which cannot be achieved using the change in electronic charge. Many years of studies have told us that reliability problems are critically important in the development of both conventional and emerging memories, in order to improve the product yield. However, the topics related to these problems are too wide to cover in these limited pages. In this review chapter, we address several interesting examples of trap-related problems in dielectrics for use in various memory cells. For engineering purposes, it is very important to grasp the relation of the achieved physical intuitions and electronic characteristics of dielectrics.
Wireless Monitoring of Biological Objects at Microwaves
Authors: Vendik Irina , Vendik Orest , Pleskachev Vladimir , Munina Irina , Turalchuk Pavel , Kirillov Vitalii
Published: May 2021

Electromagnetic (EM) wave propagation inside and along the surface of the human body is the subject of active research in the field of biomedical applications of microwaves. This research area is the basis for wireless monitoring of biological object parameters and characteristics. Solutions to the following problems are crucial for achieving the stated goals in the area of wireless monitoring: EM wave propagation inside and on-body surface. The biological object monitoring is based on a consideration of the following problems: (i) dielectric properties of a biological issue; (ii) EM wave propagation in biological medium; (iii) propagation of EM waves across the boundary of two media (biological medium–air): wave reflection and refraction; (iv) EM wave propagation in a multilayer biological medium; (v) EM wave propagation along the plane or curved surface of biological objects.
Accurate Ultrasound Indoor Localization Using Spring-Relaxation Technique
Authors: Chew Moi Tin , Alam Fakhrul , Legg Mathew , Sen Gupta Gourab
Published: May 2021

This paper reports on the development of an ultrasonic sensing-based active localization system. The system employs an ultrasonic array to transmit chirp signals and time-of-flight measurement for ranging. The position of the receiver is estimated iteratively using the spring-relaxation technique. A median and 90-percentile error of 12.4 and 21.7 mm, respectively, were obtained for measurements at 625 positions within a 1.2 m × 1.2 m area testbed. The spring-relaxation technique outperforms the widely adopted linear least square-based lateration technique while using the same ranging data. The performance of the system is benchmarked against that of visible light positioning using the same platform setup. The reported results show the ultrasonic system to be more accurate when compared with the visible light positioning system, which achieved median and 90-percentile errors of 33.7 and 58.6 mm, respectively.
LED Rail Signals: Full Hardware Realization of Apparatus with Independent Intensity by Temperature Changes
Authors: Schirripa Spagnolo Giuseppe , Leccese Fabio
Published: May 2021

Nowadays, signal lights are made using light-emitting diode arrays (LEDs). These devices are extremely energy efficient and have a very long lifetime. Unfortunately, especially for yellow/amber LEDs, the intensity of the light is closely related to the junction temperature. This makes it difficult to design signal lights to be used in naval, road, railway, and aeronautical sectors, capable of fully respecting national and international regulations. Furthermore, the limitations prescribed by the standards must be respected in a wide range of temperature variations. In other words, in the signaling apparatuses, a system that varies the light intensity emitted according to the operating temperature is useful/necessary. In this paper, we propose a simple and effective solution. In order to adjust the intensity of the light emitted by the LEDs, we use an LED identical to those used to emit light as a temperature sensor. The proposed system was created and tested in the laboratory. As the same device as the ones to be controlled is used as the temperature sensor, the system is very stable and easy to set up.
Research on an Improved Sliding Mode Sensorless Six-Phase PMSM Control Strategy Based on ESO
Authors: Gao Hanying , Zhang Guoqiang , Wang Wenxue , Liu Xuechen
Published: May 2021

The six-phase motor control system has low torque ripple, low harmonic content, and high reliability; therefore, it is suitable for electric vehicles, aerospace, and other applications requiring high power output and reliability. This study presents a superior sensorless control system for a six-phase permanent magnet synchronous motor (PMSM). The mathematical model of a PMSM in a stationary coordinate system is presented. The information of motor speed and position is obtained by using a sliding mode observer (SMO). As torque ripple and harmonic components affect the back electromotive force (BEMF) estimated value through the traditional SMO, the function of the frequency-variable tracker of the stator current (FVTSC) is used instead of the traditional switching function. By improving the SMO method, the BEMF is estimated independently, and its precision is maintained under startup or variable-speed states. In order to improve the estimation accuracy and resistance ability of the observer, the rotor position error was taken as the disturbance term, and the third-order extended state observer (ESO) was constructed to estimate the rotational speed and rotor position through the motor mechanical motion equation. Finally, the effectiveness of the method is verified by simulation and experiment results. The proposed control strategy can effectively improve the dynamic and static performance of PMSM.
Pyramidal Systems in Resistance Training
Authors: Cattan Grégoire Hugues
Published: May 2021

Pyramidal systems refer to a particular type of resistance training in which sets are performed with increasing (or decreasing) weight, in such a way that the number of repetitions is low when the weight is high (and vice versa). Multiple implementations exist such as the light-to-heavy, triangle or asymmetric triangle system. They are similar to traditional training, but with slightly different impact on training volume, endurance or power outcome. Therefore, pyramidal systems are ideal candidates for practitioners willing to tune their training routine.
Botryosphaeria Dothidea and Neofusicoccum Yunnanense Causing Canker and Die-Back of Sequoiadendron Giganteum in Croatia
Authors: Kovač Marta , Diminić Danko , Orlović Saša , Zlatković Milica
Published: May 2021

Sequoiadendron giganteum Lindl. [Buchholz] is a long-lived tree species endemic to the Sierra Nevada Mountains in California. Due to its massive size and beauty, S. giganteum is a popular ornamental tree planted in many parts of the world, including Europe. Since 2017, scattered branch die-back has been observed on S. giganteum trees in Zagreb, Croatia. Other symptoms included resinous branch cankers, reddish-brown discoloration of the sapwood and, in severe cases, crown die-back. Branches showing symptoms of die-back and cankers were collected from six S. giganteum trees in Zagreb and the aim of this study was to identify the causal agent of the disease. The constantly isolated fungi were identified using morphology and phylogenetic analyses based on the internal transcribed spacer (ITS) of the ribosomal DNA (rDNA), and partial sequencing of two housekeeping genes, i.e., translation elongation factor 1-α (TEF 1-α), and β tubulin 2 (TUB2). The fungi were identified as Botryosphaeria dothidea (Moug.) Ces. and De Not. and Neofusicoccum yunnanense G.Q. Li & S.F. Chen. The pathogenicity test was conducted in a plant growth chamber on S. giganteum seedlings and revealed that N. yunnanense was more aggressive compared to B. dothidea. N. yunnanense was able to reproduce symptoms of canker and die-back and kill plants seven weeks after inoculation whereas B. dothidea produced cankers. To the best of our knowledge, this is the first report of B. dothidea and N. yunnanense causing canker and die-back disease of S. giganteum in Croatia. It is also the first record on the identity and pathogenicity of any fungal species associated with S. giganteum in this country. The study expended the known host range of N. yunnanense to include S. giganteum, which is a valuable ornamental tree in Croatian landscapes. Disease management strategies should be developed to mitigate or reduce the impact of the disease.
Spatial Optimization and Tradeoffs of Alternative Forest Management Scenarios in Macedonia, Greece
Authors: Palaiologou Palaiologos , Kalabokidis Kostas , Ager Alan A. , Galatsidas Spyros , Papalampros Lampros , Day Michelle A.
Published: May 2021

Managing forests has been demonstrated to be an efficient strategy for fragmenting fuels and for reducing fire spread rates and severity. However, large-scale analyses to examine operational aspects of implementing different forest management scenarios to meet fire governance objectives are nonexistent for many Mediterranean countries. In this study we described an optimization framework to build forest management scenarios that leverages fire simulation, forest management, and tradeoff analyses for forest areas in Macedonia, Greece. We demonstrated the framework to evaluate five forest management priorities aimed at (1) protection of developed areas, (2) optimized commercial timber harvests, (3) protection of ecosystem services, (4) fire resilience, and (5) reducing suppression difficulty. Results revealed that by managing approximately 33,000 ha across all lands in different allocations of 100 projects, the area that accounted for 16% of the wildfire exposure to developed areas was treated while harvesting 2.5% of total wood volume. The treatments also reduced fuels on the area that are responsible for 3% of the potential fire impacts to sites with important ecosystem services, while suppression difficulty and wildfire transmission to protected areas attainment was 4.5% and 16%, respectively. We also tested the performance of multiple forest district management priorities when applying a proposed four-year fuel treatment plan that targeted achieving high levels of attainment by treating less area but strategically selected lands. Sharp management tradeoffs were observed among all management priorities, especially for harvest production compared with suppression difficulty, the protection of developed areas, and wildfire exposure to protected areas.
Fire History (1896–2013) in an Abies religiosa Forest in the Sierra Norte of Puebla, Mexico
Authors: Cerano-Paredes Julián , Rodríguez-Trejo Dante A. , Iniguez José M. , Cervantes-Martínez Rosalinda , Villanueva-Díaz José , Franco-Ramos Osvaldo
Published: May 2021

The oyamel forests, as Abies dominated forests are commonly known as, register their largest distribution (95% of their population) along the Trans-Mexican Volcanic Belt (TMVB). Although efforts have been made to study these forests with various approaches, dendrochronology-based studies have been limited, particularly in pure Abies forests in this region. The objective of this study was to reconstruct fire regimes in an Abies religiosa forest in the Sierra Norte in the state of Puebla, Mexico. Within an area of 50-ha, we collected 40 fire-scar samples, which were processed and analyzed using dendrochronological techniques to identify 153 fire scars. The fire history was reconstructed for a period of 118 years (1896–2013), with low severity surface fires occurring mainly during in the spring (92.8%) and summer (7.2%). Over the past century, fires were frequent, with an mean fire interval (MFI) and Weibull median probability of (WMPI) of five years when considering all fire scars and less than 10 years for fires covering larger areas (fires recorded by ≥25% of samples). Extensive fires were synchronized with drought conditions based on Ring Width Indexes, Palmer Drought Severity Index (PDSI) and El Niño Southern Oscillation (ENSO). After 1983, we observed a change in fire frequencies attributed to regulated management. Longer fire intervals within the last several decades are likely leading to increased fuel accumulations and could potentially result in more severe fires in the future, threatening the sustainability of these forests. Based on our finding, we recommend management actions (silvicultural or prescribed fire) to reduce fuels and the risk of severe fires, particularly in the face of climatic changes.
Effect of UV-C Irradiation and Lactic Acid Application on the Inactivation of Listeria monocytogenes and Lactic Acid Bacteria in Vacuum-Packaged Beef
Authors: Brugnini Giannina , Rodríguez Soledad , Rodríguez Jesica , Rufo Caterina
Published: May 2021

The objective of this study was to test the effect of the combined application of lactic acid (0–5%) (LA) and UV-C light (0–330 mJ/cm2) to reduce Listeria monocytogenes and lactic acid bacteria (LAB) on beef without major meat color (L *, a *, b *) change and its impact over time. A two-factor central composite design with five central points and response surface methodology (RSM) were used to optimize LA concentration and UV-C dose using 21 meat pieces (10 g) inoculated with L. monocytogenes (LM100A1). The optimal conditions were analyzed over 8 weeks. A quadratic model was obtained that predicted the L. monocytogenes log reduction in vacuum-packed beef treated with LA and UV-C. The maximum log reduction for L. monocytogenes (1.55 ± 0.41 log CFU/g) and LAB (1.55 ± 1.15 log CFU/g) with minimal impact on meat color was achieved with 2.6% LA and 330 mJ/cm2 UV-C. These conditions impaired L. monocytogenes growth and delayed LAB growth by 2 weeks in vacuum-packed meat samples throughout 8 weeks at 4 °C. This strategy might contribute to improving the safety and shelf life of vacuum-packed beef with a low impact on meat color.
Subcritical Water Extraction and Hydrolysis of Cod (Gadus morhua) Frames to Produce Bioactive Protein Extracts
Authors: Melgosa Rodrigo , Marques Marta , Paiva Alexandre , Bernardo Ana , Fernández Naiara , Sá-Nogueira Isabel , Simões Pedro
Published: May 2021

The valorization of Atlantic cod (Gadus morhua) frames from a filleting industry was investigated using subcritical water extraction and hydrolysis (SBW) at different temperatures (90, 140, 190 and 250 °C) and 100 bar to obtain extracts rich in proteins, peptides and amino acids. Up to 57.7 g of extract per 100 g of codfish frames were obtained, with nearly total recovery of the protein fraction. At each temperature, protein extracts of decreasing molecular weight were obtained, according to SEC-GPC results. Most of the protein present in the raw material and extracts was collagen and collagen fragments, as suggested by the amino acid profile. Codfish SBW extracts did not show cytotoxicity in the range of concentrations tested and the protein extract obtained at the lowest temperature (90 °C) showed the highest anti-inflammatory potential in human intestinal epithelium cell model. The mineralized residue left after SBW treatment of cod frames was identified as practically pure, crystalline, hydroxyapatite, that may find applications in biomedical field and hard-tissue engineering. This study shows the possible valorization of cod frames using green extraction methods such as SBW process to obtain protein extracts for food and nutraceutical applications.
Safety of Alternative Proteins: Technological, Environmental and Regulatory Aspects of Cultured Meat, Plant-Based Meat, Insect Protein and Single-Cell Protein
Authors: Hadi Joshua , Brightwell Gale
Published: May 2021

Food security and environmental issues have become global crises that need transformative solutions. As livestock production is becoming less sustainable, alternative sources of proteins are urgently required. These include cultured meat, plant-based meat, insect protein and single-cell protein. Here, we describe the food safety aspects of these novel protein sources, in terms of their technological backgrounds, environmental impacts and the necessary regulatory framework for future mass-scale production. Briefly, cultured meat grown in fetal bovine serum-based media can be exposed to viruses or infectious prion, in addition to other safety risks associated with the use of genetic engineering. Plant-based meat may contain allergens, anti-nutrients and thermally induced carcinogens. Microbiological risks and allergens are the primary concerns associated with insect protein. Single-cell protein sources are divided into microalgae, fungi and bacteria, all of which have specific food safety risks that include toxins, allergens and high ribonucleic acid (RNA) contents. The environmental impacts of these alternative proteins can mainly be attributed to the production of growth substrates or during cultivation. Legislations related to novel food or genetic modification are the relevant regulatory framework to ensure the safety of alternative proteins. Lastly, additional studies on the food safety aspects of alternative proteins are urgently needed for providing relevant food governing authorities with sufficient data to oversee that the technological progress in this area is balanced with robust safety standards.
The Effect of Water Mineralization on the Extraction of Active Compounds from Selected Herbs and on the Antioxidant Properties of the Obtained Brews
Authors: Wyrostek Jakub , Kowalski Radosław
Published: May 2021

The objective of the study was to analyze the effect of total content of minerals in waters on the effectiveness of extraction of polyphenols and flavonoids and on the antioxidant properties of brews from leaves of green and black tea, leaves of peppermint, chamomile inflorescences, leaves of sage, and flowers of lavender. The process of brewing was conducted at an initial temperature of 95 °C, under cover, for 15 min, with the use of 10 waters differing in terms of mineral components. The content of total polyphenols and total flavonoids was determined in the brews obtained. The antioxidant properties were assayed with the use of stable DPPH radicals and Ferric Reducing Antioxidant Power (FRAP) reagent. A significant effect of water mineralization on the extraction of phenolic compounds and on the antioxidant properties of the brews obtained was observed. In the majority of cases, the highest concentrations of polyphenols and flavonoids were obtained in brews prepared with the use of deionized water and waters with medium levels of mineralization. It was also demonstrated that there was a significant reduction of antioxidant properties in brews prepared with the water that had the highest level of mineralization (2896 ppm), at 70% and 93%, respectively, for green and black teas.