bluuebunny/crossref_metadata_2025 huggingface dataset:
Validating RRP12 Expression and Its Prognostic Significance in HCC Based on Data Mining and Bioinformatics Methods
Authors: Wei Chao , Wang Ben , Chen Zhong-Huo , Xiao Han , Tang Lei , Guan Jia-Fu , Yuan Rong-Fa , Yu Xin , Hu Zhi-Gang , Wu Hua-Jun , Dai Zhi , Wang Kai
Published: February 2022

RRP12 (ribosomal RNA processing 12 homolog) is a nucleolar protein involved in the maturation and transport of eukaryotic ribosomal subunits and is a type of RNA binding protein. In recent years, considerable research has indicated that RRP12 is associated with the occurrence and development of multiple cancers. However, there is no research on RRP12 in hepatocellular carcinoma. Herein, we aimed to explore the role and significance of RRP12 in hepatocellular carcinoma.We used the TIMER and GEPIA databases to perform pan-cancer analyses of RRP12. The impact of RRP12 on the prognosis was analyzed through the GEPIA database. The relationship between RRP12 and immune cell infiltration was investigated by TIMER and GEPIA databases. Moreover, the expression of RRP12 in various liver cancer cells was evaluated by Western Blot to determine the cell line for the next experiment. Scratch test, Transwell test, and Edu tests were applied to validate the effects of RRP12 on the function of liver cancer cells. And the data were statistically analyzed.Pan-cancer analysis found that RPP12 was significantly upregulated in many cancers. Moreover, the prognostic analysis revealed that the difference in the expression of RRP12 has statistical significance for the overall survival rate and disease-free survival rate of liver cancer patients. In order to analyze the correlation between the expression level of RRP12 and clinical parameters, it was found that there was a significant negative correlation with tumor stage, tumor grade and tumor size. Univariate and multivariate analysis showed that RRP12 could be used as an independent prognostic factor for patients with hepatocellular carcinoma. Cellular experiments have proved that knocking down RRP12 can inhibit the proliferation, invasion, and metastasis of liver cancer cells.Therefore, RRP12 significantly affects the occurrence and development of HCC. Hence, RRP12 can become a potential target and prognostic biomarker for the treatment of hepatocellular carcinoma.
Hinokiflavone Inhibits Growth of Esophageal Squamous Cancer By Inducing Apoptosis via Regulation of the PI3K/AKT/mTOR Signaling Pathway
Authors: Guo Jida , Zhang Shengqiang , Wang Jun , Zhang Pengfei , Lu Tong , Zhang Linyou
Published: February 2022

Background

Globally, esophageal cancer ranks as the seventh most common cancer. Esophageal squamous cell carcinoma (ESCC) is one of its major histological types. ESCC accounts for the vast majority of cases in China, and the mortality rate is high. Cisplatin, the standard adjuvant chemotherapy drug for ESCC, has a modest response rate due to the development of drug resistance. Hinokiflavone (HF) is a natural biflavonoid compound with anti-melanoma activity. However, its anti-tumor effect on ESCC and the underlying mechanisms remain largely unknown.

Methods

The ESCC cell lines KYSE150 and TE14 were used. The cell counting kit-8 assay and flow cytometry analysis, along with colony formation, EdU, wound healing, and Transwell migration assays, were performed to assess cell characteristics (viability, migration, invasion, and apoptosis) following treatment with HF. Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), western blotting, and molecular docking were used to investigate the pathways potentially modulated by HF. In vivo anti-tumor effects of HF were also investigated using a mouse xenograft model.

Results

Our findings revealed that HF inhibited ESCC cell proliferation. Hoechst 33342 staining, annexin V-FITC/PI staining, and western blotting confirmed that HF causes caspase-dependent apoptosis. KEGG pathway enrichment analysis and western blotting indicated that the PI3K/AKT/mTOR pathway played an important role in the process of HF-induced apoptosis. Furthermore, HF effectively impaired the migration and invasion abilities of KYSE150 cells and downregulated the expression of the matrix metalloproteinases (MMP) MMP2 and MMP9. HF inhibited tumor growth and exhibited minimal toxicity in the organs of the KYSE150 xenograft model.

Conclusion

This is the first study to demonstrate the inhibition of ESCC growth and progression by HF. The underlying mechanism is through blocking the PI3K/AKT/mTOR signaling pathway, thereby inhibiting cell proliferation and inducing apoptosis. HF can be used as a complementary/alternative agent for ESCC therapy.
Noise-Induced Aggregation of Swimmers in the Kolmogorov Flow
Authors: Berman Simon A. , Ferguson Kyle S. , Bizzak Nathaniel , Solomon Thomas H. , Mitchell Kevin A.
Published: February 2022

We investigate a model for the dynamics of ellipsoidal microswimmers in an externally imposed, laminar Kolmogorov flow. Through a phase-space analysis of the dynamics without noise, we find that swimmers favor either cross-stream or rotational drift, depending on their swimming speed and aspect ratio. When including noise, i.e., rotational diffusion, we find that swimmers are driven into certain parts of phase space, leading to a nonuniform steady-state distribution. This distribution exhibits a transition from swimmer aggregation in low-shear regions of the flow to aggregation in high-shear regions as the swimmer’s speed, aspect ratio, and rotational diffusivity are varied. To explain the nonuniform phase-space distribution of swimmers, we apply a weak-noise averaging principle that produces a reduced description of the stochastic swimmer dynamics. Using this technique, we find that certain swimmer trajectories are more favorable than others in the presence of weak rotational diffusion. By combining this information with the phase-space speed of swimmers along each trajectory, we predict the regions of phase space where swimmers tend to accumulate. The results of the averaging technique are in good agreement with direct calculations of the steady-state distributions of swimmers. In particular, our analysis explains the transition from low-shear to high-shear aggregation.
A Review on Laser-Induced Breakdown Spectroscopy in Different Cancers Diagnosis and Classification
Authors: Khan Muhammad Nouman , Wang Qianqian , Idrees Bushra Sana , Xiangli Wenting , Teng Geer , Cui Xutai , Zhao Zhifang , Wei Kai , Abrar Muhammad
Published: February 2022

Due to the new demonstrations of Laser-induced breakdown spectroscopy (LIBS) applicability in a surprisingly wide variety of applications, the use of LIBS as a medical diagnostic tool is steadily gaining momentum. Especially in different cancer diseases, LIBS has the potential to become a fast and valuable analytical tool. We addressed LIBS equipment and quantitative analytical procedures, and signal enhancement techniques for improving element detection. For detailed aspects of applications, we reviewed the recent progress of LIBS in different cancer diseases diagnoses by using different tissues and medical fluid as samples. To fulfill the high demands in the medical industry and overcome the severe tissue sample problem, it is proposed that the chemometric and signal amplification techniques for quantitative analysis should be employed, and robust and effective LIBS devices should be developed. This overview of the different cancers by LIBS is meant to summarize the research performed to date and suggest some suitable advanced chemometrics techniques and effective LIBS devices, if successfully implemented, would be significantly beneficial to the medical field in the future.
Tracing Mobile DNAs: From Molecular to Population Scales
Authors: Fan Wenwen , Wang Ling , Chu Jie , Li Hui , Kim Eun Yu , Cho Jungnam
Published: February 2022

Transposable elements (TEs, transposons) are mobile DNAs that are prevalent in most eukaryotic genomes. In plants, their mobility has vastly contributed to genetic diversity which is essential for adaptive changes and evolution of a species. Such mobile nature of transposon has been also actively exploited in plant science research by generating genetic mutants in non-model plant systems. On the other hand, transposon mobilization can bring about detrimental effects to host genomes and they are therefore mostly silenced by the epigenetic mechanisms. TEs have been studied as major silencing targets and acted a main feature in the remarkable growth of the plant epigenetics field. Despite the importance of transposon in plant biology and biotechnology, their mobilization and the underlying mechanisms are largely left unanswered. This is mainly because of the sequence repetitiveness of transposons, which makes their detection and analyses difficult and complicated. Recently, some attempts have been made to develop new experimental methods detecting active transposons and their mobilization behavior. These techniques reveal TE mobility in various levels, including the molecular, cellular, organismal and population scales. In this review, we will highlight the novel technical approaches in the study of mobile genetic elements and discuss how these techniques impacted on the advancement of transposon research and broadened our understanding of plant genome plasticity.
The Spanish Language as a Cultural and Touristic Resource for the Chinese Market to Develop Quality Education
Authors: García-Henche Blanca , Yang Ming
Published: February 2022

Since 1952, Spanish has been included as a Degree in the Foreign Language Studies in the higher education system of China. The number of Spanish students has gradually increased and, until March 2020, with 6 Universities recently approved by the Chinese Ministry of Education (MOE), there are 102 Chinese universities that teach Spanish as a university degree. In 2017, the MOE of the People's Republic of China (PRC) published the Curriculum Plan in the Higher Secondary Schools, which incorporated the teaching of Spanish as a teaching option along with other languages, such as English, Japanese, Russian, German, and French. In the following year, during the Chinese President's state visit to Spain, a cooperation agreement was signed between the Royal Spanish Academy (abbreviated as RAE for its Spanish name) and SISU, under which a RAE-SISU Joint Research Centre was established. This study aims to publicize the situation of Spanish teaching in China, showing how a language can become a cultural and language tourism resource for Spain. An empirical study of student profiles was carried out at one of the most prestigious universities with reference to Spanish teaching in China, SISU. The data were obtained through primary sources employing a survey sent to the Chinese students studying their bachelor's degree of Spanish Philology, obtaining a sample of 159 students. The results revealed information on the studies carried out, also reviewing the most popular universities among the Chinese students in Spain, their stay period, and the destination image that the students have. The data will serve to show the opportunities for Spain as a destination for language tourism for the Chinese market. One of the implications of this study would be that it helps to explain how Spain can design a product for a niche market that demands Spain as a destination for language and cultural tourism.
A Modified Two-Layer Suture Technique for Transperitoneal Laparoscopic Partial Nephrectomy: Single-Center Clinical Experience
Authors: Jin Yang , Xiong Hui , Xia Qinghua , Zhang Qi
Published: February 2022

Purposes

This study aims to evaluate the feasibility and efficacy of a modified two-layer suture method during laparoscopic partial nephrectomy (LPN) by a comparative analysis with the traditional two-layer suture.

Methods

A total of 60 LPN patients were enrolled in this study, of which 30 patients received the modified two-layer suture method and the remaining 30 patients underwent the traditional two-layer suture. Then, surgical characteristics including operative time, warm ischemic time (WIT), estimated blood loss (EBL), and glomerular filtration rate (GFR) were recorded. Finally, univariable and multivariable linear regression analyses were used to evaluate the correlations of tumor characteristics, suture methods, and postoperative renal function.

Results

There was no significant difference between the two suture groups with respect to patient and tumor characteristics, postoperative creatinine level, and blood urea nitrogen (BUN) level. The modified suture group showed a significantly shorter clamping time and a less GFR level reduction than the traditional two-layer suture group (15 vs. 23 min; 42.32 ± 9.48 vs. 27.07 ± 7.88; p < 0.05). Additionally, the modified two-layer suture was an independent factor that influenced the clamping time and the level of GFR reduction.

Conclusion

The modified two-layer suture method is feasible and effective for LPN.
Evaluation of YouTube Videos as a Source of Patient Information for Ureteric Stent Placement: A Quality Assessment Study
Authors: Chaudhary Kapil , Chandna Abhishek , Kumar Devana Sudheer , Sharma Aditya Prakash , Tyagi Shantanu , Singh Shrawan K.
Published: February 2022

Objective

To assess the quality of YouTube videos on ureteric stent placement (USP) as a source of patient available.

Methods

YouTube was searched using search terms “DJ stenting,” “Double J stenting,” and “ureteric stenting.” The initial 100 videos displayed with each of the above mentioned search terms were scrutinized. The selected videos reviewed by 3 independent consultant urologists against a pre-agreed scoring system based upon European Association of Urology (EAU) patient information sheet on ureteric stent placement. The videos were scored qualitatively and quantitatively based on the scores achieved in various domains of the scoring Performa. Data was also collected for the number of views, likes, dislikes, and time duration of each video.

Results

A total of 22 videos which fulfilled the inclusion criteria were reviewed. All the videos were uploaded by healthcare organizations or healthcare websites. None of the videos were classified as “Good” based on reviewer scores and only one video was classified as “acceptable.” Fourteen videos were classified as “very poor” with a score of <5/20. General information about stents was described by majority of the studies whilst preoperative information, procedure description, danger signs, and follow up were scarcely described by most videos.

Conclusion

Majority of YouTube videos on USP are of poor overall quality and lack pertinent information. This calls for creation of comprehensive and unbiased videos for patient information on USP.
The Role of Consumer-Citizens and Connectedness to Nature in the Sustainable Transition to Agroecological Food Systems: The Mediation of Innovative Business Models and a Multi-Level Perspective
Authors: Mehrabi Sepide , Perez-Mesa Juan Carlos , Giagnocavo Cynthia
Published: February 2022

Conventional agricultural systems have contributed to social, economic and environmental problems and are the main threat to global sustainability. In response, theoretical frameworks to describe the transition to sustainable food systems have been proposed, emphasizing the necessity to shift from farm-level solutions to a focus on interactions within the entire value chain, from production to consumption. Despite the emphasis on the importance and potential of consumers to contribute to sustainable agri-food transitions, approaches to their role have remained within the traditional, linear supply chain framework. Marketing approaches view consumers as passive actors, limited to voting with their wallets, which has deepened the disconnection between consumers, producers and nature, resulting in a triple fracture. We analyze the role of the consumer in agri-food systems, contrasting marketing approaches with other consumers/citizens concepts and locate them within sustainability transition frameworks and a multi-level perspective. We discuss the re-establishment of the connection between farmers and consumers and human–nature connectedness and explore this connection mediated through innovative business models, which act as niche innovations with the capacity to influence regimes and landscapes within the multi-level perspective. The role of consumers/citizens in the co-creation of innovative business models is also addressed.
A High Sensitivity Electrochemical Immunosensor Based on Monoclonal Antibody Coupled Flower-Shaped Nano-ZnO for Detection of Tenuazonic Acid
Authors: Zhang Chi , Du Congcong , Liu Wei , Guo Ting , Zhou Ying , Zhou Hongyuan , Zhang Yuhao , Liu Xiaozhu , Ma Liang
Published: February 2022

In this paper, an electrochemical biosensor was established for the high-sensitivity detection of Tenuazonic acid (TeA) in fruits based on the enrichment of flower-shaped nano-ZnO and the specific recognition of immune response. Herein flower-shaped nano-ZnO (ZnO NFs) with a hexagonal wurtzite structure and diameter of 700–800 nm were demonstrated to have the optimal specific surface area and outstanding conductivity, compared with different morphology, sizes, and crystal structures of nano-ZnO. Second, the ZnO NFs were used as carriers for efficiently immobilizing monoclonal antibodies to obtain antibody bioconjugates, which were anchored on the 2-mercaptobenzoic acid-modified gold electrode by amide reaction. In the presence of TeA, the monoclonal antibody could specifically recognize and bind to it, resulting in a decrease in electron transfer ability on the gold electrode surface. Finally, the electrochemical biosensor showed a range from 5 × 10−5 μg/mL to 5 × 10−1 μg/mL with a detection limit of 1.14 × 10−5 μg/mL. Furthermore, it exhibited high selectivity for TeA among other analogs, such as Altenuene (ALT) and Alternariol (AOH). Notably, the proposed strategy could be employed to monitor TeA in tomato and citrus, showing potential application prospects in practical application and commercial value.
Response of Indian Dwarf Wheat and Persian Wheat to Sowing Density and Hydrothermal Conditions of the Growing Seasons
Authors: Szczepanek Małgorzata , Lemańczyk Grzegorz , Nowak Rafał , Graczyk Radomir
Published: February 2022

The need for foods with high nutritional value has led to the rediscovery of ancient wheat species Triticum sphaerococcum and T. persicum as raw materials with valuable consumption properties, but their reintroduction requires assessment of their productivity under different agricultural practices. The field experiments were carried out for three years (2018–2020) to test the hypothesis that the sowing density of T. sphaerococcum and T. persicum (400, 500 and 600 no m−2) will affect their agronomic traits, yield and occurrence of diseases, but the response will depend on the hydrothermal conditions of the growing seasons. In this study, a significant correlation of the grain yield with the amount of precipitation in tillering, and from booting to the beginning of fruit development was demonstrated. The sowing density of T. sphaerococcum had an impact on the grain yield only under moderate drought stress during the growing season (2019), when the highest yield was obtained at a sowing density of 600 m−2. In 2019, the most favorable sowing density was also the highest for T. persicum. In the year with the lowest amount of rainfall during the growing season (2018), the yield of T. persicum was the highest in the lowest sowing density. At the shooting stage, a greater intensity of powdery mildew was observed on T. persicum, especially with higher sowing densities. Increasing the sowing density also increased the occurrence of root rot symptoms in both wheat species in the year that favored the occurrence of this disease (2018). It can be concluded that in the integrated low-input cultivation of T. sphaerococcum and T. persicum, it is justified to use a sowing density of 600 pcs. m−2, in an agroclimatic zone with moderate droughts during the growing season.
The Eco-Efficiency of Castor Supply Chain: A Greek Case Study
Authors: Pari Luigi , Alexopoulou Efthymia , Stefanoni Walter , Latterini Francesco , Cavalaris Chris , Palmieri Nadia
Published: February 2022

Castor is a candidate crop that grows in marginal lands in the Mediterranean area. It can be grown by utilizing minimal resources to provide unique industrial chemicals or to serve as an energy crop. However, it can be also cultivated with intensive inputs providing higher yields. Whether a high input or a low input scheme is more sustainable depends on the economic and environmental impacts of each case. The objective of the present study, therefore, was to evaluate these impacts under the Mediterranean climate and farming conditions by examining two alternative scenarios: a castor crop grown on a low-inputs field vs. a crop grown in a high-inputs one. The environmental impacts were estimated by following a Life Cycle Assessment (LCA) methodology based on GHG emissions. Furthermore, a sensitivity analysis was performed by switching the functional unit from 1 Mg of castor oil to 1 hectare. The economic feasibility of the castor crop production was assessed by calculating the gross margin, which is referred to the difference between revenues and the variable costs due to the agricultural phases. In addition, the ratio between gross margin and GWP (Global Warming Potential) emissions was applied to calculate the economic performance (gross margin) per unit of environmental burden. Findings showed that the castor oil produced by high inputs resulted in a more sustainable scenario due to its higher yield than low-inputs ones. On the other hand, sensitivity analysis showed that the field management with low inputs showed GHG emissions that were 27% lower than those emitted from the field management with high inputs. Moreover, from an economic point of view, by switching the field management from low inputs to high ones, the Gross Margin increased by about 73%. Finally, the high-inputs scenario showed the best ratio between economic performance and GHG emitted into the atmosphere.
Exploring the Adaptive Responses of Plants to Abiotic Stresses Using Transcriptome Data
Authors: Javaid Muhammad Haseeb , Khan Ali Raza , Salam Abdul , Neelam Asifa , Azhar Wardah , Ulhassan Zaid , Gan Yinbo
Published: February 2022

In recent decades, global climate change and heavy metal stress have severely affected plant growth and biomass, which has led to a serious threat to food safety and human health. Anthropogenic activities, the rapid pace of urbanization, and the use of modern agricultural technologies have further aggravated environmental conditions, resulting in limited crop growth and productivity. This review highlights the various adaptive transcriptomic responses of plants to tolerate detrimental environmental conditions, such as drought, salinity, and heavy metal contamination. These stresses hinder plant growth and development by disrupting their physiological and biochemical processes by inducing oxidative stress, nutritional imbalance, and osmotic disturbance, and by deteriorating their photosynthetic machinery. Plants have developed different strategies to safeguard themselves against the toxic effects of these environmental stresses. They stimulate their secondary messenger to activate cell signaling, and they trigger other numerous transcriptomic responses associated with plant defense mechanisms. Therefore, the recent advances in biological sciences, such as transcriptomics, metabolomics, and proteomics, have assisted our understanding of the stress-tolerant strategies adopted by plants, which could be further utilized to breed tolerant species. This review summarizes the stress-tolerant strategies of crops by covering the role of transcriptional factors in plants.
Phytoremediation of Secondary Salinity in Greenhouse Soil with Astragalus sinicus, Spinacea oleracea and Lolium perenne
Authors: Cai Shumei , Xu Sixin , Zhang Deshan , Fu Zishi , Zhang Hanlin , Zhu Haitao
Published: February 2022

Phytoremediation is an effective and ecological method used to control soil secondary salinization in greenhouses. However, the plant–soil interactions for phytoremediation have not been studied sufficiently. In this study, three crop species (Astragalus sinicus (CM), Spinacea oleracea (SP) and Lolium perenne (RY)) were compared in a greenhouse experiment. The results showed that all three crops increased the soil microbial biomass, the abundance of beneficial microorganisms, available phosphorus and soil pH, and reduced the soil salt content. The crop nutrient accumulation was positively correlated with the relative abundance of bacterial 16S rRNA sequences in the soil. CM and RY respectively increased the relative abundances of norank_f_Gemmatimonadaceae and norank_f_Anaerolineaceae within the soil bacterial community, while SP increased the relative abundances of Gibellulopsis within the fungal community. Correlation analysis revealed that pH and total dissolved salts were the vital factors affecting soil microbial communities in the secondary salinized soil. Our results suggest that phytoremediation could effectively alleviate secondary salinization by regulating the balance of soil microbial community composition and promoting crop nutrient accumulation.
Device and Method Suitable for Matching and Adjusting Reel Speed and Forward Speed of Multi-Crop Harvesting
Authors: Ji Kuizhou , Li Yaoming , Liang Zhenwei , Liu Yanbin , Cheng Junhui , Wang Hanhao , Zhu Ruiheng , Xia Shengbo , Zheng Guoqiang
Published: February 2022

In order to meet the requirements of multi-crop harvesting, reduce the loss of harvesting, and improve the quality of harvesting, the device and method of matching and adjusting the rotation speed and forward speed of the pulling reel were designed. On the premise of satisfying the matching regulation, the parameters of the rotary wheel and the cutting table were adjusted so that the rotational speed ratio λ of the harvest crop was in the suitable range of the rotational speed ratio of the crop. The speed ratio was designed as a suitable interval of different crops by experiments and experience, and, in order to meet the requirements, it was designed to optimize the wheel speed. The speed-matching was designed, and through the experiments on the wheel-speed-adjustment error, which was less than 2%, it was designed to meet the design requirements. Crop-harvesting experiments were carried out under rotational speed-matching and a random speed, and the experimental results showed that the loss rate under rotational speed-matching was significantly lower than that under random speeds; the tests showed that the wheel speed designed with speed-matching can effectively reduce the loss rate.
Effect of pH on Schizochytrium limacinum Production Grown Using Crude Glycerol and Biogas Digestate Effluent
Authors: Bouras Sofoklis , Antoniadis Dimitrios , Kountrias Georgios , Karapanagiotidis Ioannis T. , Katsoulas Nikolaos
Published: February 2022

The ability of microalgae, such as the rich in docosahexaenoic acid (DHA) heterotrophic marine microalgae Schizochytrium limacinum SR21, to utilize nutrients in their culture media derived from low-cost nutrient sources makes them a promising low-cost alternative source for the production of useful substances used in aqua feeds. The assessment of culture parameters, one of which is the pH, for every different nutrient growth medium used for microalgae cultivation is important, as they affect the biomass and secondary metabolite microalgae production. This study assessed the effect of different growth medium pH levels (6, 7, 8 and 9), at laboratory and pilot scale systems, on S. limacinum biomass productivity, lipid accumulation, proximate composition, carbon assimilation and DHA. The microalgae were cultivated in growth media containing two different alternative low-cost nutrient sources: (a) crude glycerol derived from biofuel industry as carbon source and (b) effluent digestate from biogas production of livestock decomposition as a source of nutrients and trace elements. It was found that a neutral pH (7) was the optimum level, as it enhanced biomass productivity of the lab and pilot scale cultivation systems at 44.9 g L−1 and 11 g L−1 and DHA content at 7.5% and 19% of the total lipid content, respectively.
Plant Disease Recognition Model Based on Improved YOLOv5
Authors: Chen Zhaoyi , Wu Ruhui , Lin Yiyan , Li Chuyu , Chen Siyu , Yuan Zhineng , Chen Shiwei , Zou Xiangjun
Published: February 2022

To accurately recognize plant diseases under complex natural conditions, an improved plant disease-recognition model based on the original YOLOv5 network model was established. First, a new InvolutionBottleneck module was used to reduce the numbers of parameters and calculations, and to capture long-distance information in the space. Second, an SE module was added to improve the sensitivity of the model to channel features. Finally, the loss function ‘Generalized Intersection over Union’ was changed to ‘Efficient Intersection over Union’ to address the former’s degeneration into ‘Intersection over Union’. These proposed methods were used to improve the target recognition effect of the network model. In the experimental phase, to verify the effectiveness of the model, sample images were randomly selected from the constructed rubber tree disease database to form training and test sets. The test results showed that the mean average precision of the improved YOLOv5 network reached 70%, which is 5.4% higher than that of the original YOLOv5 network. The precision values of this model for powdery mildew and anthracnose detection were 86.5% and 86.8%, respectively. The overall detection performance of the improved YOLOv5 network was significantly better compared with those of the original YOLOv5 and the YOLOX_nano network models. The improved model accurately identified plant diseases under natural conditions, and it provides a technical reference for the prevention and control of plant diseases.
Connecting Nature: The Potential of Australian Dairy Initiatives in Collaborative Biodiversity Governance
Authors: Cosby Amy , Lawson Andrew , Gudde Jane , Fogarty Eloise S.
Published: February 2022

The dairy industry can be considered a contributor to biodiversity loss in Australia. To address this, many forms of governance can be enlisted, including traditional legislation and regulations, persuasive techniques such as publicly funded subsidy programs or education, and participation in voluntary stewardship programs. This paper explores the benefits of collaborative governance programs, which have international applications to reduce the impact of the dairy industry on biodiversity loss. However, as the Australian sector is unique, the specific opportunities and present challenges are discussed. This paper reports three important objectives that could underpin industry-led initiatives by supporting improved biodiversity conservation on dairy farms: (1) Increase the personal and financial capacities of individual farmers to operate profitable, biodiverse farms; (2) Facilitate market rewards to incentivise pro-conservation behaviours; and (3) Improve the effectiveness of the implementation of biodiversity protection laws and regulatory objectives via collaborative governance arrangements. Existing environmental programs that have been developed by the dairy industry could be suitable for incorporation into more formal co-governance structures sympathetic to biodiversity conservation. However, to be successful in addressing sustainability issues, including biodiversity loss, strengthening the integrity mechanisms around farmers’ self-reporting of performance is required to ensure that the industry can credibly refute claims of greenwashing and defend their environmental credentials in the global marketplace.
Beneficial Soil Fungi and Jabuticaba Growth Promotion
Authors: Batistello Maira Nascimento , Brito Nayane Fonseca , de Sousa Willian Nogueira , Felsemburgh Cristina Aledi , Vieira Thiago Almeida , Lustosa Denise Castro
Published: February 2022

Trichoderma is a genus of fungi widely used in agriculture as a biocontrol agent and more recently as a plant growth promoter. We evaluated five Trichoderma isolates, in different application modes, throughout the development of jabuticaba seedlings. These included three isolates of the species Trichoderma asperellum (Tam01, Tam02, and Tam03) and two isolates of Trichoderma sp. (Tc and Tce) in four modes of application: via seeds; in applications on the pre-planting substrate; in monthly applications in the post-planting substrate; and their combination. The control treatment consisted of plants without the use of Trichoderma. Seedling height, collar diameter, and the number of leaves were evaluated monthly. Statistical analysis was conducted using data collected two, four, seven, and thirteen months after emergence. The application of Trichoderma promoted the growth of jabuticaba seedlings up to four months after emergence. The isolate Tam03 and the application via seeds were the treatments that most influenced the analyzed variables at four months. The use of Trichoderma stimulated the beginning of the development of jabuticaba seedlings.
Hydrolyzed Yeast Supplementation to Newly Weaned Piglets: Growth Performance, Gut Health, and Microbial Fermentation
Authors: Boontiam Waewaree , Bunchasak Chaiyaphum , Kim Yoo Yong , Kitipongpysan Sumetee , Hong Jinsu
Published: February 2022

Hydrolyzed yeast (HY)-derived protein from Saccharomyces cerevisiae has a high digestible protein content and nucleotides and is a sweetener immunostimulatory substance. This could be used in nursery diets to minimize diarrhea and improve the growth rate and gut health of early-weaned piglets. This research was conducted with the objective of examining the effect of the inclusion level of HY as a potential protein ingredient for early-weaned piglets. A total of 72 crossbred weaned piglets [(Landrace × Large White) × Duroc] were assigned to three dietary treatments in six replicates with four pigs per pen. Dietary treatments were: (i) control (CON), piglets weaned at 18 days; (ii) CON diet with 5% HY inclusion (HY5); and (iii) CON diet with 10% HY inclusion (HY10) in a corn–soybean meal-based basal diet. Increasing HY levels positively improved body weight, average daily gain, and average daily feed intake (linear effect, p < 0.05). Furthermore, there was a linear increase in N-retention, albumin, jejunal villus height, villus height-to-crypt depth ratio, immunoglobulin A, acetate and propionate production, and Lactobacillus spp. count proportional to the dose of the HY-supplemented diet (p < 0.05). It also observed a decrease in diarrheal rate, jejunal crypt depth, blood urea nitrogen, pro-inflammatory cytokines, branched amino acids, and E. coli corresponding to the HY-supplemented levels (p < 0.05). However, the changes in the apparent total tract digestibility (dry matter, crude ash, and crude fat), blood glucose, butyrate, and Salmonella spp. were unaffected by the dietary HY level. Therefore, the supplementation of HY in the diet for early-weaned pigs not only supported the growth rate and immune function but also activated the beneficial bacterial growth of the early-weaned piglets.
Response of Long-Tailed Duck (Clangula hyemalis) to the Change in the Main Prey Availability in Its Baltic Wintering Ground
Authors: Forni Paola , Morkūnas Julius , Daunys Darius
Published: February 2022

The long-tailed duck (Clangula hyemalis) is a vulnerable and declining species wintering in the Baltic Sea. The introduction of the invasive fish, the round goby (Neogobius melanostomus), dramatically impacted the benthic macrofauna in hard-bottom habitats, while no significant changes occurred in soft-bottom benthic macrofauna. Therefore, we aimed to assess the extent to which the diet of long-tailed ducks changed in two different bottom types. We analysed the stomach content of 251 long-tailed ducks bycaught in gillnets from 2016 to 2020 in hard- and soft-bottom habitats and compared these results with those published by Žydelis and Ruškyte (2005). The results show that the long-tailed duck experienced a change in diet in hard-bottom habitats, shifting from the blue mussel to Hediste diversicolor, barnacles, and fish. In soft-bottom habitats, their diet remained similar over time and was based on H. diversicolor, a few bivalve species, and Saduria entomon. There was no evidence of significant differences in diet between sex or age. Despite the abovementioned changes in diet, the average body condition of the species did not change over time or between habitats. This confirms that long-tailed ducks have high feeding flexibility and quick species response to changes in prey availability, as they are capable of shifting their diet to new prey.
Navel Healing and Calf Fitness for Transport
Authors: Roccaro Mariana , Bolcato Marilena , Masebo Naod Thomas , Gentile Arcangelo , Peli Angelo
Published: February 2022

Dairy male calves are at risk of welfare compromise as they are usually transported at a very young age. The European Union has set a “completely healed navel” requirement for calf transport; moreover, a minimum age is established for longer journeys. However, this requirement has proven to be prone to misinterpretation. This study aimed to clarify what is meant by “navel healing” and to provide strong elements for reaching a consensus. The navels of 299 dairy calves (55 males, 244 females) aged 0–90 days were examined and scored 1 to 5 according to their healing status. Based on our results, a completely dry and shriveled navel (score 3) would imply a 25.5–38.0% risk of transporting too young calves. Alternatively, the presence of a scab covering the umbilical wound (score 4) would entail a 4.3% risk of transporting calves less than 10 days old and could be considered good practice for transporting calves (except for journeys exceeding 8 h). Conversely, complete navel healing (score 5) guarantees that calves that are too young are not transported; therefore, it should be considered best practice for transporting calves in general and the minimum requirement for transporting calves for journeys exceeding 8 h.
Antimicrobial Activity of Eucalyptus globulus, Azadirachta indica, Glycyrrhiza glabra, Rheum palmatum Extracts and Rhein against Porphyromonas gingivalis
Authors: Müller-Heupt Lena Katharina , Vierengel Nina , Groß Jonathan , Opatz Till , Deschner James , von Loewenich Friederike D.
Published: February 2022

Novel plant-derived antimicrobials are of interest in dentistry, especially in the treatment of periodontitis, since the use of established substances is associated with side effects and concerns of antimicrobial resistance have been raised. Thus, the present study was performed to quantify the antimicrobial efficacy of crude plant extracts against Porphyromonas gingivalis, a pathogen associated with periodontitis. The minimal inhibitory concentrations (MICs) of Eucalyptus globulus leaf, Azadirachta indica leaf, Glycyrrhiza glabra root and Rheum palmatum root extracts were determined by broth microdilution for P. gingivalis ATCC 33277 according to CLSI (Clinical and Laboratory Standards Institute). The MICs for the E. globulus, A. indica and G. glabra extracts ranged from 64 mg/L to 1024 mg/L. The lowest MIC was determined for an ethanolic R. palmatum extract with 4 mg/L. The MIC for the anthraquinone rhein was also measured, as the antimicrobial activity of P. palmatum root extracts can be partially traced back to rhein. Rhein showed a remarkably low MIC of 0.125 mg/L. However, the major compounds of the R. palmatum root extract were not further separated and purified. In conclusion, R. palmatum root extracts should be further studied for the treatment of periodontitis.
A Review on the Antimicrobial Activity of Schiff Bases: Data Collection and Recent Studies
Authors: Ceramella Jessica , Iacopetta Domenico , Catalano Alessia , Cirillo Francesca , Lappano Rosamaria , Sinicropi Maria Stefania
Published: February 2022

Schiff bases (SBs) have extensive applications in different fields such as analytical, inorganic and organic chemistry. They are used as dyes, catalysts, polymer stabilizers, luminescence chemosensors, catalyzers in the fixation of CO2 biolubricant additives and have been suggested for solar energy applications as well. Further, a wide range of pharmacological and biological applications, such as antimalarial, antiproliferative, analgesic, anti-inflammatory, antiviral, antipyretic, antibacterial and antifungal uses, emphasize the need for SB synthesis. Several SBs conjugated with chitosan have been studied in order to enhance the antibacterial activity of chitosan. Moreover, the use of the nanoparticles of SBs may improve their antimicrobial effects. Herein, we provide an analytical overview of the antibacterial and antifungal properties of SBs and chitosan-based SBs as well as SBs-functionalized nanoparticles. The most relevant and recent literature was reviewed for this purpose.
Overview of Methods for Large-Scale RNA Synthesis
Authors: Ryczek Marcin , Pluta Martyna , Błaszczyk Leszek , Kiliszek Agnieszka
Published: February 2022

In recent years, it has become clear that RNA molecules are involved in almost all vital cellular processes and pathogenesis of human disorders. The functional diversity of RNA comes from its structural richness. Although composed of only four nucleotides, RNA molecules present a plethora of secondary and tertiary structures critical for intra and intermolecular contacts with other RNAs and ligands (proteins, small metabolites, etc.). In order to fully understand RNA function it is necessary to define its spatial structure. Crystallography, nuclear magnetic resonance and cryogenic electron microscopy have demonstrated considerable success in determining the structures of biologically important RNA molecules. However, these powerful methods require large amounts of sample. Despite their limitations, chemical synthesis and in vitro transcription are usually employed to obtain milligram quantities of RNA for structural studies, delivering simple and effective methods for large-scale production of homogenous samples. The aim of this paper is to provide an overview of methods for large-scale RNA synthesis with emphasis on chemical synthesis and in vitro transcription. We also present our own results of testing the efficiency of these approaches in order to adapt the material acquisition strategy depending on the desired RNA construct.
Design of Titanium Alloy Femoral Stem Cellular Structure for Stress Shielding and Stem Stability: Computational Analysis
Authors: Al Zoubi Naser Fawzi , Tarlochan Faris , Mehboob Hassan , Jarrar Firas
Published: February 2022

The main objective of this study is to design titanium alloy femoral stems with cubic porous structures that will be able to reduce stress shielding and promote stem stability. These porous structure designs were introduced into titanium alloy femoral stems as homogeneous and functionally graded porous structures. First, the cubic cellular structures were simulated under compressive loading to measure the yield and modulus of elasticity for various porosity ranges. Based on the selected porosity range, fifteen different arrangements of radial geometrical functionally graded (FG) designs were developed with average porosities of 30, 50, and 70% respectively. Finite element models were developed with physiological loads presenting three different walking speeds (1, 3, and 5 km/h), where the average human body weight was assumed. Stresses at the bone Gruen zones were measured to check the percentage of stress transfer to the bone for each porous stem design and were compared with the bulk stem. Several FG stem designs were shortlisted for further investigation as candidates for hip implants.
Improving the Forecasting Performance of Taiwan Car Sales Movement Direction Using Online Sentiment Data and CNN-LSTM Model
Authors: Ou-Yang Chao , Chou Shih-Chung , Juan Yeh-Chun
Published: February 2022

The automotive industry is the leading producer of machines in Taiwan and worldwide. Developing effective methods for forecasting car sales can allow car companies to arrange their production and sales plans. Capitalizing on the growth of social media and deep learning algorithms, this research aimed to improve the overall performance of the forecasting of Taiwan car sales movement direction forecasting by using online sentiment data and CNN-LSTM method. First, the historical sales volumes and multi-channel online sentiment data for six car brands in Taiwan were collected and preprocessed for labeling of car sales movement direction. Then, three models, namely, the classical, sentimental, and CNN-LSTM models, were constructed and trained/fitted for forecasting car sales movement directions in Taiwan. Finally, the performance of the three prediction models were compared to verify the effects of online sentiment data and the CNN-LSTM model on forecasting performance. The results showed that four forecasting performance indices, i.e., accuracy, precision, recall and F1-score, improved by 27.78% (from 41.67% to 69.45%), 0.39 (from 0.38 to 0.77), 0.27 (from 0.42 to 0.69) and 0.33 (from 0.35 to 0.68), respectively. Therefore, the online sentiment data and CNN-LSTM method can indeed improve the overall performance of car sales movement direction in Taiwan.
Coverage Fulfillment Automation in Hardware Functional Verification Using Genetic Algorithms
Authors: Danciu Gabriel Mihail , Dinu Alexandru
Published: February 2022

The functional verification process is one of the most expensive steps in integrated circuit manufacturing. Functional coverage is the most important metric in the entire verification process. By running multiple simulations, different situations of DUT functionality can be encountered, and in this way, functional coverage fulfillment can be improved. However, in many cases it is difficult to reach specific functional situations because it is not easy to correlate the required input stimuli with the expected behavior of the digital design. Therefore, both industry and academia seek solutions to automate the generation of stimuli to reach all the functionalities of interest with less human effort and in less time. In this paper, several approaches inspired by genetic algorithms were developed and tested using three different designs. In all situations, the percentage of stimulus sets generated using well-performing genetic algorithms approaches was higher than the values that resulted when random simulations were employed. In addition, in most cases the genetic algorithm approach reached a higher coverage value per test compared to the random simulation outcome. The results confirmed that in many cases genetic algorithms can outperform constrained random generation of stimuli, that is employed in the classical way of doing verification, considering coverage fulfillment level per verification test.
The Emerging Role of Cyclodextrin Metal–Organic Frameworks in Ostheotherapeutics
Authors: Braga Susana Santos , Paz Filipe A. Almeida
Published: February 2022

Metal–Organic Frameworks (MOFs) are a class of coordination compounds with high versatility of design and a high loading ability. These properties have made them increasingly attractive for drug delivery, with research focusing mostly on the preparation of biocompatible MOFs. A suitable strategy is the use of cyclodextrins, cyclic oligosaccharides with excellent biosafety profiles. This review summarises the early steps taken in the application of cyclodextrin-coated and cyclodextrin-based MOFs in the treatment of bone-related diseases divided into three categories: cyclodextrin-coated MOFs, cyclodextrin-based MOFs and cross-linked derivatives of CD-MOFs. Applications in the delivery of bisphosphonates, osteoporotic regulating drugs and non-steroidal anti-inflammatory drugs, for osteoarthritis management, are highlighted. Lastly, the use of these materials in dentistry is exemplified with a recent report.