bluuebunny/crossref_metadata_2025 huggingface dataset:
Study on the State of Methane Molecule Adsorption on Different Media in Highly Evolved Marine Shales—A Case Study on the Shales From the Lower Silurian Longmaxi Formation in the Sichuan Basin, Southern China
Authors: Jiang Lin , Song Yan , Liu Wenping , Chen Zhiyuan , Zhang Hanbing , He Fangyu
Published: January 2022

The major sedimentary basins in China contain abundant shale gas resources to be explored, and the exploration of shale gas has received more attention in recent years. Shale gas exists mainly in two states, i.e., free and adsorbed. The latter mainly exists on the surface of media, including organic matter and clay minerals, etc., but its adsorption state remains unknown. In this paper, we take the Longmaxi Formation marine shale in the southern Sichuan Basin of southern China as the research object. The state of methane molecule adsorption on different media in marine shales is analyzed by conducting mineral composition analysis, TOC content analysis, isothermal adsorption experiments, FIB-SEM, and FIB-HIM experiments on the core samples referring to previous research. The conclusions are as follows: the adsorbed gas mainly exists in the organic-matter pores, which feature excellent roundness and connectivity with a large number of small pores inside like a hive. The surface of the organic matter contains many adsorption sites, featuring strong adsorption capacity and making methane molecules continuously distributed on the internal surface of the organic-matter pores. The organic matter has a large specific surface area and is lipophilic, which offers an ideal condition for the adsorption of methane molecules. Part of the adsorbed gas exists in the pores of clay minerals, which are lamellar and triangular. The surface of clay minerals contains fewer adsorption sites, featuring poorer adsorption capacity and making methane molecules discontinuously distributed on the surface of the clay minerals. The clay minerals have a smaller specific surface area than the organic matter, thus featuring a smaller space for adsorption. The clay minerals are hydrophilic. In addition to methane molecules, mixed-layer illite/smectite (I/S) and chlorite also adsorb water molecules. The illite surface adsorbs mainly water molecules and, to a lesser extent, methane molecules. Finally, the adsorption state patterns of methane molecules on organic matter and clay minerals were summarized.
Factorial Invariance and Internal Structure of the Scale of the Students’ Role in the Cycle of School Violence (ERECVE)
Authors: Pérez-Morán Juan C. , Rodríguez-Macías Juan C.
Published: January 2022

The purpose of this study was to analyze evidence of construct validity of internal structure and factorial invariance for the Scale of the Students’ Role in the Cycle of School Violence (ERECVE, in Spanish). To that end, we relied on a database of 13,389 participants with a sample of Mexican low secondary education students: 6,935 female and 6,454 male. Participants had a mean age of 13.08 years (SD = 0.98). The dimensionality and fit of a reconfigured five-factor model were analyzed using Confirmatory Factor Analysis (CFA); nested models sequencing methods were applied subsequently to validate invariance between genders. The results from the dimensionality analysis support the reconfigured five-factor structure for the dimensions referring to the roles of victim, defender or conciliator, sole bully, social bully and bystander. Moreover, acceptable fit indices were obtained for the configurational, weak, strong and strict models after comparing the nested models. It was concluded that the reconfigured five-factor model is useful for measuring the roles of students in the cycle of school violence, and that the ERECVE achieves a simultaneous measurement invariance, thereby favoring the analysis of mean differences between genders.
Positive Effects on Alfalfa Productivity and Soil Nutrient Status in Coastal Wetlands Driven by Biochar and Microorganisms Mixtures
Authors: Cui Qian , Xia Jiangbao , Peng Ling , Zhao Ximei , Qu Fanzhu
Published: January 2022

Biochar application in reclaiming degraded soils and improving plant productivity has been recognized as a promising technology. Yet, the impacts of biochar and mixtures with compound effective microorganisms (CEM) on alfalfa growth and soil quality in coastal wetlands are poorly understood. A greenhouse experiment was set to systematically reveal the impacts of biochar and biochar combined with CEM on alfalfa growth traits, nutrient uptake, biomass, soil quality, and enzyme activities. Eight treatments were included: (1) control (CK−CEM), (2) 10-g/kg biochar (B10−CEM); (3) 20-g/kg biochar (B20−CEM); (4) 30-g/kg biochar (B30−CEM), (5) CEM without biochar (CK + CEM); (6) 10-g/kg biochar with CEM (B10 + CEM), (7) 20-g/kg biochar with CEM (B20 + CEM), (8) 30-g/kg biochar with CEM (B30 + CEM). The utilization of biochar promoted seed germination, height, and tissue nutrient contents of alfalfa, and the combined biochar with CEM showed greater effects. Alfalfa biomass showed the maximum value in the B20 + CEM treatment, and the biomass of root, shoot, leaf in the B20 + CEM treatment increased by 200, 117.3, 144.6%, respectively, relative to the CK−CEM treatment. Alfalfa yield in the CK + CEM, B10 + CEM, B20 + CEM, B30 + CEM treatments was 71.91, 84.11, 138.5, and 120.5% higher than those in the CK−CEM treatment. The use of biochar and CEM decreased soil salinity and elevated soil nutrient content effectively. Biochar elevated soil organic carbon (SOC) and microbial biomass carbon (MBC), NH4+, NO3–, and enzymatic activities, and the positive impacts of biochar combined with CEM were additive. The combined addition of 20-g/kg biochar with CEM showed the pronounced improvement effects on improving soil fertility and nutrient availability as well as soil enzyme activities. Path analysis indicated that the application of biochar mixture with CEM promoted alfalfa biomass by regulating plant nutrient uptake, soil quality (soil nitrogen, SOC, MBC, NH4+, NO3–), and soil enzymatic activities (sucrase, urease, and alkaline phosphatases). Thus, incorporation of suitable biochar and CEM can serve as an effective measure to promote alfalfa productivity and restore coastal wetlands soils.
How Organisms Come to Know the World: Fundamental Limits on Artificial General Intelligence
Authors: Roli Andrea , Jaeger Johannes , Kauffman Stuart A.
Published: January 2022

Artificial intelligence has made tremendous advances since its inception about seventy years ago. Self-driving cars, programs beating experts at complex games, and smart robots capable of assisting people that need care are just some among the successful examples of machine intelligence. This kind of progress might entice us to envision a society populated by autonomous robots capable of performing the same tasks humans do in the near future. This prospect seems limited only by the power and complexity of current computational devices, which is improving fast. However, there are several significant obstacles on this path. General intelligence involves situational reasoning, taking perspectives, choosing goals, and an ability to deal with ambiguous information. We observe that all of these characteristics are connected to the ability of identifying and exploiting new affordances—opportunities (or impediments) on the path of an agent to achieve its goals. A general example of an affordance is the use of an object in the hands of an agent. We show that it is impossible to predefine a list of such uses. Therefore, they cannot be treated algorithmically. This means that “AI agents” and organisms differ in their ability to leverage new affordances. Only organisms can do this. This implies that true AGI is not achievable in the current algorithmic frame of AI research. It also has important consequences for the theory of evolution. We argue that organismic agency is strictly required for truly open-ended evolution through radical emergence. We discuss the diverse ramifications of this argument, not only in AI research and evolution, but also for the philosophy of science.
Small Open Reading Frames, How to Find Them and Determine Their Function
Authors: Kute Preeti Madhav , Soukarieh Omar , Tjeldnes Håkon , Trégouët David-Alexandre , Valen Eivind
Published: January 2022

Advances in genomics and molecular biology have revealed an abundance of small open reading frames (sORFs) across all types of transcripts. While these sORFs are often assumed to be non-functional, many have been implicated in physiological functions and a significant number of sORFs have been described in human diseases. Thus, sORFs may represent a hidden repository of functional elements that could serve as therapeutic targets. Unlike protein-coding genes, it is not necessarily the encoded peptide of an sORF that enacts its function, sometimes simply the act of translating an sORF might have a regulatory role. Indeed, the most studied sORFs are located in the 5′UTRs of coding transcripts and can have a regulatory impact on the translation of the downstream protein-coding sequence. However, sORFs have also been abundantly identified in non-coding RNAs including lncRNAs, circular RNAs and ribosomal RNAs suggesting that sORFs may be diverse in function. Of the many different experimental methods used to discover sORFs, the most commonly used are ribosome profiling and mass spectrometry. These can confirm interactions between transcripts and ribosomes and the production of a peptide, respectively. Extensions to ribosome profiling, which also capture scanning ribosomes, have further made it possible to see how sORFs impact the translation initiation of mRNAs. While high-throughput techniques have made the identification of sORFs less difficult, defining their function, if any, is typically more challenging. Together, the abundance and potential function of many of these sORFs argues for the necessity of including sORFs in gene annotations and systematically characterizing these to understand their potential functional roles. In this review, we will focus on the high-throughput methods used in the detection and characterization of sORFs and discuss techniques for validation and functional characterization.
Comparison of the Agronomic, Cytological, Grain Protein Characteristics, as Well as Transcriptomic Profile of Two Wheat Lines Derived From Wild Emmer
Authors: Gong Fangyi , Qi Tiangang , Zhang Tian , Lu Yusen , Liu Jia , Zhong Xiaoying , He Jingshu , Li Yunfang , Zheng Youliang , Liu Dengcai , Huang Lin , Wu Bihua
Published: January 2022

Two advanced wheat lines BAd7-209 and BAd23-1 without the functional gene GPC-B1 were obtained from a cross between common wheat cultivar Chuannong 16 (CN16) and wild emmer wheat accession D97 (D97). BAd7-209 showed superior quality parameters than those of BAd23-1 and CN16. We found that the components of glutenins and gliadins in BAd7-209 and BAd23-1 were similar, whereas BAd7-209 had higher amount of glutenins and gliadins than those of BAd23-1. RNA sequencing analysis on developing grains of BAd7-209 and BAd23-1 as well as their parents revealed 382 differentially expressed genes (DEGs) between the high–grain protein content (GPC) (D97 + BAd7-209) and the low-GPC (CN16 + BAd23-1) groups. DEGs were mainly associated with transcriptional regulation of the storage protein genes, protein processing in endoplasmic reticulum, and protein export pathways. The upregulated gluten genes and transcription factors (e.g., NAC, MYB, and bZIP) may contribute to the high GPC in BAd7-209. Our results provide insights into the potential regulation pathways underlying wheat grain protein accumulation and contribute to make use of wild emmer for wheat quality improvement.
Insights Into the Emergence of Paroxysmal Nocturnal Hemoglobinuria
Authors: Colden Melissa A. , Kumar Sushant , Munkhbileg Bolormaa , Babushok Daria V.
Published: January 2022

Paroxysmal Nocturnal Hemoglobinuria (PNH) is a disease as simple as it is complex. PNH patients develop somatic loss-of-function mutations in phosphatidylinositolN-acetylglucosaminyltransferase subunit A gene (PIGA), required for the biosynthesis of glycosylphosphatidylinositol (GPI) anchors. Ubiquitous in eukaryotes, GPI anchors are a group of conserved glycolipid molecules responsible for attaching nearly 150 distinct proteins to the surface of cell membranes. The loss of two GPI-anchored surface proteins, CD55 and CD59, from red blood cells causes unregulated complement activation and hemolysis in classical PNH disease. In PNH patients,PIGA-mutant, GPI (-) hematopoietic cells clonally expand to make up a large portion of patients’ blood production, yet mechanisms leading to clonal expansion of GPI (-) cells remain enigmatic. Historical models of PNH in mice and the more recent PNH model in rhesus macaques showed that GPI (-) cells reconstitute near-normal hematopoiesis but have no intrinsic growth advantage and do not clonally expand over time. Landmark studies identified several potential mechanisms which can promote PNH clonal expansion. However, to what extent these contribute to PNH cell selection in patients continues to be a matter of active debate. Recent advancements in disease models and immunologic technologies, together with the growing understanding of autoimmune marrow failure, offer new opportunities to evaluate the mechanisms of clonal expansion in PNH. Here, we critically review published data on PNH cell biology and clonal expansion and highlight limitations and opportunities to further our understanding of the emergence of PNH clones.
Low Resighting Rate of Entangled Humpback Whales Within the California, Oregon, and Washington Region Based on Photo-Identification and Long-Term Life History Data
Authors: Tackaberry Jennifer , Dobson Elana , Flynn Kiirsten , Cheeseman Ted , Calambokidis John
Published: January 2022

Bycatch, commonly referred to as entanglement, is a leading source of human-caused mortality of baleen whales. A better understanding of the individuals that survive (or perish from) their entanglement can help reduce the risk of bycatch by informing gear modifications and fisheries management. However, determining survival rates is restricted by the ability to track individuals once they become entangled. Historically, the effort to identify and resight individuals from known entanglement cases was low along the West Coast. The recent increase of entanglements and photo-identification efforts in the California, Oregon, and Washington region provides an opportunity to assess entangled humpback whales’ resighting rates to better understand the effect of entanglements from the individual to the population level. We used photo-ID images of entangled humpback whales between 1982 and 2017 to examine pre- and post-entanglement sighting histories from longitudinal catalogs and life history data (Cascadia Research and Happywhale). We compared the entangled whales (n= 37) against control whales (n= 2,296), selected based on the date and location of the entanglement reports, to evaluate the deviation from the expected mortality rates (or lack of resighting) caused by entanglements and to help inform/support/test National Oceanic and Atmospheric Association’s Serious Injury and Mortality (SI/M) index. Our results suggest that entangled whales were resighted less often than the control groups. Entangled whales with short pre-entanglement sighting histories and without post-entanglement resights did not match other feeding ground populations. Therefore, the higher proportion of entanglements with shorter sighting histories is likely due to their being alive for fewer years, indicating a higher risk of entanglement for younger whales. This indicates that entangled humpback whales may not be as large or strong as mature individuals, and future gear modifications should reflect that possibility. Additionally, the severity of the initial SI/M score aligned well with our resighting rates, though this worsened with the final score assigned. Continued effort to gather and improve data collection about entanglements will help enhance the SI/M determinations. Our findings show the value of photo-identification of entangled whales and how it dramatically increases our understanding of entanglements.
Variable-Density Flow and Solute Transport in Stratified Salt Marshes
Authors: Wu Xiaojing , Wang Yuansheng , Shen Chengji , Zhao Zhongwei
Published: January 2022

Subsurface hydrodynamics underpin the eco-functions of salt marshes. Many studies have investigated these processes under various conditions. However, the impact of soil stratification (a low-permeability mud layer overlying a high-permeability sand layer) on the variable-density groundwater flow (particularly unstable flow) and solute transport in regularly tide-flooded marshes remains poorly understood. The present study numerically explored this question based on a 2D cross-creek section of salt marshes, by comparing cases with and without stratification. Results show that, the low-permeability mud layer delays the initiation of unstable flow and leads to smaller and denser salt fingers. Consequently, solute plume stays in the marsh soil for a longer time and spreads more widely than that in the homogeneous case. Also, soil stratigraphy extends the duration and shrinks the zone of solute discharge across the tidal creek. Sensitivity analysis was conducted based on three key controlling variables: hydraulic conductivity contrast between mud layer and sand layer (Kmud/Ksand), salinity contrast between surface water and groundwater (Csea/Cpore), and mud layer thickness (Dmud). The results demonstrate that the residence time of solute plume in a two-layered salt marsh is less sensitive to Csea/Cpore than to Kmud/Ksand and Dmud. Moreover, the commencement and duration of solute discharge are more sensitive to Kmud/Ksand and Dmud than to Csea/Cpore. While the location of solute discharge zone is highly sensitive to Dmud and slightly influenced by Kmud/Ksand and Csea/Cpore. Findings from this study would facilitate a deeper understanding of the eco-functions of salt marshes.
A Two-Dimensional Assessment of China’s Approach Concerning the Compensation Fund for Ship-Induced Oil Pollution Damage: Pollution Governance and Victim Compensation
Authors: Cao Xingguo , Chang Yen-Chiang
Published: January 2022

China has adopted a domestic approach to the compensation fund for ship-induced oil pollution damage by establishing the Chinese Ship-induced Oil Pollution Compensation (CSOPC) Fund. After almost 10 years of practice, there are still opinions and questions regarding the rationality of China’s domestic approach. This study reassesses China’s domestic approach by a two-dimensional analysis of the current position and potential developments of the CSOPC Fund’s functions in pollution governance and victim compensation. At the present stage, the goal of adequate compensation to victims is not well achieved by the CSOPC Fund when compared to its other goal of ensuring a better oil pollution emergency reaction. Given that the International Oil Pollution Compensation (IOPC) Fund is unlikely to contribute much to pollution governance, and the current inadequacy and defects of the CSOPC Fund can be improved gradually, it is suggested that China should continue its domestic approach with necessary improvements. Using the CSOPC Fund’s practice as a case study, this research intends to explore the pollution governance side of the compensation fund that has not yet been fully addressed, demonstrating the key value of the domestic fund compared to the IOPC Fund.
Static and Kinetic Friction From Nanoscale Slip—A Multiscale Approach Using a 2D Binary Hierarchy of Nodes
Authors: Streator Jeffrey L.
Published: January 2022

A local, elastic deformation model is combined with a dynamic simulation to investigate nanoscale slip between a rigid, curved pin and an elastic slab, and its influence on static and kinetic friction. The elastic deformation model utilizes a novel multiscale grid based on a binary hierarchy. To maximize accuracy, bi-quadratic functions are introduced to interpolate the stresses on the boundaries of the nodal elements. The onset of slip is based on a maximum allowable nodal shear stress to nodal pressure ratio. A nanoscale friction function is developed by translating the pin quasistatically across the slab. The effect of the nanoscale friction profile on a dynamic system is investigated by integrating the equations of motions governing the pin as it is pulled by a stage via a coupling spring. A direct connection is found between the nanoscale slip characteristics and macroscopically observed static and kinetic coefficients of friction.
Tailoring Ferrimagnetic Transition Temperatures, Coercivity Fields, and Saturation Magnetization by Modulating Mn Concentration in (CoCrFeNi)1−xMnx High-Entropy Alloys
Authors: Lee Chi-Hung , Chin Hsu-Hsuan , Zeng Kun-Yuan , Chang Yao-Jen , Yeh An-Chou , Yeh Jien-Wei , Lin Su-Jien , Wang Chun-Chieh , Glatzel Uwe , Huang E-Wen
Published: January 2022

Cr and Mn play intriguing roles in determining the magnetic properties of CoFeNi-based high-entropy alloys (HEA). In this study, we tune the stoichiometric Mn composition to systematically explore the magnetic properties of (CoCrFeNi)1−xMnx HEAs. We observe a change from ferro-to ferrimagnetism due to the incorporation of Mn atoms into the CoCrFeNi HEA. In addition, we measure an 81% reduction in magnetization with the incorporation of 7.6 (2)% Mn atoms. Such a significant reduction in magnetization cannot be solely explained by the effect of the inversed moments on the Mn atoms. Hence, we propose a mechanism whereby the Mn atoms flip the moments of neighboring atoms, which results in the magnetization reduction observed in the CoFeNi-based HEAs.
The Utility of a Point-of-Care Transcranial Doppler Ultrasound Management Algorithm on Outcomes in Pediatric Asphyxial Out-of-Hospital Cardiac Arrest – An Exploratory Investigation
Authors: Lin Jainn-Jim , Kuo Hsuan-Chang , Hsia Shao-Hsuan , Lin Ying-Jui , Wang Huei-Shyong , Hsu Mei-Hsin , Chiang Ming-Chou , Chan Oi-Wa , Lee En-Pei , Lin Kuang-Lin
Published: January 2022

Background

Transcranial Doppler ultrasound is a sensitive, real time tool used for monitoring cerebral blood flow; it could provide additional information for cerebral perfusion in cerebral resuscitation during post cardiac arrest care. The aim of the current study was to evaluate the utility of a point-of-care transcranial Doppler ultrasound management algorithm on outcomes in pediatric asphyxial out-of-hospital cardiac arrest.

Methods

This retrospective cohort study was conducted in two tertiary pediatric intensive care units between January 2013 and June 2018. All children between 1 month and 18 years of age with asphyxial out-of-hospital cardiac arrest and a history of at least 3 min of chest compressions, who were treated with therapeutic hypothermia and survived for 12 h or more after the return of circulation were eligible for inclusion.

Results

Twenty-one patients met the eligibility criteria for the study. Sixteen (76.2%) of the 21 children were male, and the mean age was 2.8 ± 4.1 years. Seven (33.3%) of the children had underlying disorders. The overall 1-month survival rate was 52.4%. Twelve (57.1%) of the children received point-of-care transcranial Doppler ultrasound. The 1-month survival rate was significantly higher (p = 0.03) in the point-of-care transcranial Doppler ultrasound group (9/12, 75%) than in the non-point-of-care transcranial Doppler ultrasound group (2/9, 22.2%).

Conclusions

Point-of-care transcranial Doppler ultrasound group was associated with a significantly better 1-month survival rate compared with no point-of-care transcranial Doppler ultrasound group in pediatric asphyxial out-of-hospital cardiac arrest.
Novel Possible Protein Targets in Neovascular Age-Related Macular Degeneration: A Pilot Study Experiment
Authors: Coronado Bruno Nobre Lins , da Cunha Felipe Bruno Santos , de Oliveira Raphaela Menezes , Nóbrega Otávio de Toledo , Ricart Carlos André Ornelas , Fontes Wagner , de Sousa Marcelo Valle , de Ávila Marcos Pereira , Martins Aline Maria Araújo
Published: January 2022

Age-related macular degeneration (AMD) is among the world's leading causes of blindness. In its neovascular form (nAMD), around 25% of patients present further anatomical and visual deterioration due to persistence of neovascular activity, despite gold-standard treatment protocols using intravitreal anti-VEGF medications. Thus, to comprehend, the molecular pathways that drive choroidal neoangiogenesis, associated with the vascular endothelial growth factor (VEGF), are important steps to elucidate the mechanistic events underneath the disease development. This is a pilot study, a prospective, translational experiment, in a real-life context aiming to evaluate the protein profiles of the aqueous humor of 15 patients divided into three groups: group 1, composed of patients with nAMD, who demonstrated a good response to anti-VEGF intravitreal injections during follow-up (good responsive); group 2, composed of patients with anti-VEGF-resistant nAMD, who demonstrated choroidal neovascularization activity during follow-up (poor/non-responsive); and group 3, composed of control patients without systemic diseases or signs of retinopathy. For proteomic characterization of the groups, mass spectrometry (label-free LC-MS/MS) was used. A total of 2,336 proteins were identified, of which 185 were distinctly regulated and allowed the differentiation of the clinical conditions analyzed. Among those, 39 proteins, including some novel ones, were analyzed as potential disease effectors through their pathophysiological implications in lipid metabolism, oxidative stress, complement system, inflammatory pathways, and angiogenesis. So, this study suggests the participation of other promising biomarkers in neovascular AMD, in addition to the known VEGF.
Current State and Issues of Regenerative Medicine for Rheumatic Diseases
Authors: Yoshimi Ryusuke , Nakajima Hideaki
Published: January 2022

The prognosis of rheumatic diseases is generally better than that of malignant diseases. However, some cases with poor prognoses resist conventional therapies and cause irreversible functional and organ damage. In recent years, there has been much research on regenerative medicine, which uses stem cells to restore the function of missing or dysfunctional tissues and organs. The development of regenerative medicine is also being attempted in rheumatic diseases. In diseases such as systemic sclerosis (SSc), systemic lupus erythematosus (SLE), and rheumatoid arthritis, hematopoietic stem cell transplantation has been attempted to correct and reconstruct abnormalities in the immune system. Mesenchymal stem cells (MSCs) have also been tried for the treatment of refractory skin ulcers in SSc using the ability of MSCs to differentiate into vascular endothelial cells and for the treatment of systemic lupus erythematosus SLE using the immunosuppressive effect of MSCs. CD34-positive endothelial progenitor cells (EPCs), which are found in the mononuclear cell fraction of bone marrow and peripheral blood, can differentiate into vascular endothelial cells at the site of ischemia. Therefore, EPCs have been used in research on vascular regeneration therapy for patients with severe lower limb ischemia caused by rheumatic diseases such as SSc. Since the first report of induced pluripotent stem cells (iPSCs) in 2007, research on regenerative medicine using iPSCs has been actively conducted, and their application to rheumatic diseases is expected. However, there are many safety issues and bioethical issues involved in regenerative medicine research, and it is essential to resolve these issues for practical application and spread of regenerative medicine in the future. The environment surrounding regenerative medicine research is changing drastically, and the required expertise is becoming higher. This paper outlines the current status and challenges of regenerative medicine in rheumatic diseases.
Micro-Technologies for Assessing Microbial Dynamics in Controlled Environments
Authors: Davidson Shanna-Leigh , Niepa Tagbo H. R.
Published: January 2022

With recent advances in microfabrication technologies, the miniaturization of traditional culturing techniques has provided ideal methods for interrogating microbial communities in a confined and finely controlled environment. Micro-technologies offer high-throughput screening and analysis, reduced experimental time and resources, and have low footprint. More importantly, they provide access to culturing microbes in situ in their natural environments and similarly, offer optical access to real-time dynamics under a microscope. Utilizing micro-technologies for the discovery, isolation and cultivation of “unculturable” species will propel many fields forward; drug discovery, point-of-care diagnostics, and fundamental studies in microbial community behaviors rely on the exploration of novel metabolic pathways. However, micro-technologies are still largely proof-of-concept, and scalability and commercialization of micro-technologies will require increased accessibility to expensive equipment and resources, as well as simpler designs for usability. Here, we discuss three different miniaturized culturing practices; including microarrays, micromachined devices, and microfluidics; advancements to the field, and perceived challenges.
Potential of Mesenchymal Stem Cell-Derived Exosomes as a Novel Treatment for Female Infertility Caused by Bacterial Infections
Authors: Zohrabi Marzieh , Dehghan Marvast Laleh , Izadi Mahin , Mousavi Seyed Alireza , Aflatoonian Behrouz
Published: January 2022

Neisseria gonorrhoeae and Chlamydia trachomatis are the most common causes of bacterial sexually transmitted diseases (STDs) with complications in women, including pelvic inflammatory disease (PID), ectopic pregnancy, and infertility. The main concern with these infections is that 70% of infected women are asymptomatic and these infections ascend to the upper female reproductive tract (FRT). Primary infection in epithelial cells creates a cascade of events that leads to secretion of pro-inflammatory cytokines that stimulate innate immunity. Production of various cytokines is damaging to mucosal barriers, and tissue destruction leads to ciliated epithelial destruction that is associated with tubal scarring and ultimately provides the conditions for infertility. Mesenchymal stem cells (MSCs) are known as tissue specific stem cells with limited self-renewal capacity and the ability to repair damaged tissues in a variety of pathological conditions due to their multipotential differentiation capacity. Moreover, MSCs secrete exosomes that contain bioactive factors such as proteins, lipids, chemokines, enzymes, cytokines, and immunomodulatory factors which have therapeutic properties to enhance recovery activity and modulate immune responses. Experimental studies have shown that local and systemic treatment of MSC-derived exosomes (MSC-Exos) suppresses the destructive immune response due to the delivery of immunomodulatory proteins. Interestingly, some recent data have indicated that MSC-Exos display strong antimicrobial effects, by the secretion of antimicrobial peptides and proteins (AMPs), and increase bacterial clearance by enhancing the phagocytic activity of host immune cells. Considering MSC-Exos can secrete different bioactive factors that can modulate the immune system and prevent infection, exosome therapy is considered as a new therapeutic method in the treatment of inflammatory and microbial diseases. Here we intend to review the possible application of MSC-Exos in female reproductive system bacterial diseases.
Induction of Reactive Oxygen Species Is Necessary for Efficient Onset of Cyprinid Herpesvirus 2 Replication: Implications for Novel Antiviral Strategy With Antioxidants
Authors: Lu Cuiyu , Tang Ruizhe , Su Meizhen , Zou Jixing , Lu Liqun
Published: January 2022

Cyprinid herpesvirus 2 (CyHV-2) has caused great economic loss to the crucian carp breeding industry. Upon viral stimulation, eukaryotic cells generally activate the expression of anti-oxidative genes to maintain the intracellular oxidative balance and resist viral infection. Here, intracellular reactive oxygen species (ROS) levels in CyHV-2-infected cells were monitored to show that CyHV-2 induced the increase of intracellular ROS during early infection, and intracellular excessive accumulation of ROS was ameliorated during late infection, which was accompanied by activated expression of genes related to Nrf2 signaling pathway. In order to explore the interaction between CyHV-2 infection and ROS production, RyuF-2 cells were treated with either antioxidant epigallocatechin-3-gallate (EGCG) or berberine hydrochloride (BBH) and then infected with CyHV-2. Both BBH and EGCG could effectively inhibit the amplification of CyHV-2 while inhibiting the accumulation of intracellular ROS. Consistent with this, the oxidant stress-related genes were up-regulated by CyHV-2 infection and down-regulated in cells treated with either BBH or EGCG, through which the production of intracellular ROS was modulated. These results collectively demonstrated that early ROS accumulation favored the replication of CyHV-2, while antioxidants (BBH and EGCG) could inhibit the amplification of CyHV-2 by inhibiting ROS induction.
An Integrative Approach to Study Bacterial Enzymatic Degradation of Toxic Dyes
Authors: Mishra Arti , Takkar Simran , Joshi Naveen Chandra , Shukla Smriti , Shukla Kartikeya , Singh Anamika , Manikonda Anusha , Varma Ajit
Published: January 2022

Synthetic dyes pose a large threat to the environment and consequently to human health. Various dyes are used in textile, cosmetics, and pharmaceutical industries, and are released into the environment without any treatment, thus adversely affecting both the environment and neighboring human populations. Several existing physical and chemical methods for dye degradation are effective but have many drawbacks. Biological methods over the years have gained importance in the decolorization and degradation of dye and have also overcome the disadvantages of physiochemical methods. Furthermore, biological methods are eco-friendly and lead to complete decolorization. The mechanism of decolorization and degradation by several bacterial enzymes are discussed in detail. For the identification of ecologically sustainable strains and their application at the field level, we have focused on bioaugmentation aspects. Furthermore, in silico studies such as molecular docking of bacterial enzymes with dyes can give a new insight into biological studies and provide an easy way to understand the interaction at the molecular level. This review mainly focuses on an integrative approach and its importance for the effective treatment and decolorization of dyes.
A Case Series and Literature Review of Vertebral Artery Stump Syndrome
Authors: Zhang Wenbin , Wang Shouchun , Li Chao , Wang Zhongxiu , Yue Feixue , Zhou Jie , Song Kangjia , Wang Chao , Wang Yujiao , Shi Mingchao
Published: January 2022

Purpose

Stump syndrome refers to cerebral ischaemic symptoms caused by an embolus from a previously occluded ipsilateral artery that occludes a down-stream artery. It can be divided into two types: carotid stump syndrome and vertebral artery stump syndrome (VASS). At present, there is limited clinical experience with VASS. We aimed to propose a more precise diagnostic standard for VASS, and to share our experience with handling this condition.

Materials and Methods

We retrospectively collected data of patients who were treated with endovascular thrombectomy in the stroke centre of the First Hospital of Jilin University from January 2016 to March 2020. After screening patients with posterior-circulation acute ischaemic stroke, we selected patients who had an acute occlusion of an intracranial artery in the context of a previously occluded ipsilateral vertebral artery origin, as confirmed by digital subtraction angiography.

Results

Eleven patients met our inclusion criteria. Nine patients achieved recanalization of both extracranial and intracranial occluded arteries, one patient had modified thrombolysis in cerebral infarction grade 3, and eight patients had grade 2b. Residual stenosis of recanalized intracranial arteries was less than 30% in all cases, while three patients had embolism of distal arteries. No dissection or subarachnoid haemorrhage occurred. Two patients didn't reach vascular recanalization. Among the nine patients with recanalized artery, four had a 90-day modified Rankin Scale score ≤ 3 (favourable outcome), and four patients died; As for the two non-recanalized patients, one had a mRS score of 5 and one died.

Conclusion

VASS is a clinical syndrome caused by embolic occlusion of a distal intracranial artery occluded ipsilateral extracranial vertebral artery. Antegrade blood flow from the collateral vessels, distal embolic occlusion and mild or no residual stenosis of the occluded intracranial artery after recanalization are notable features of this clinical event. Endovascular thrombectomy may be effective for treating VASS.
Gratifying Gizmos for Research and Clinical MEG
Authors: Jousmäki Veikko
Published: January 2022

Experimental designs are of utmost importance in neuroimaging. Experimental repertoire needs to be designed with the understanding of physiology, clinical feasibility, and constraints posed by a particular neuroimaging method. Innovations in introducing natural, ecologically-relevant stimuli, with successful collaboration across disciplines, correct timing, and a bit of luck may cultivate novel experiments, new discoveries, and open pathways to new clinical practices. Here I introduce some gizmos that I have initiated in magnetoencephalography (MEG) and applied with my collaborators in my home laboratory and in several other laboratories. These gizmos have been applied to address neuronal correlates of audiotactile interactions, tactile sense, active and passive movements, speech processing, and intermittent photic stimulation (IPS) in humans. This review also includes additional notes on the ideas behind the gizmos, their evolution, and results obtained.
Walking With Horizontal Head Turns Is Impaired in Persons With Early-Stage Multiple Sclerosis Showing Normal Locomotion
Authors: Carpinella Ilaria , Gervasoni Elisa , Anastasi Denise , Di Giovanni Rachele , Tacchino Andrea , Brichetto Giampaolo , Confalonieri Paolo , Solaro Claudio , Rovaris Marco , Ferrarin Maurizio , Cattaneo Davide
Published: January 2022

Background

Turning the head while walking (an action often required during daily living) is particularly challenging to maintain balance. It can therefore potentially reveal subtle impairments in early-stage people with multiple sclerosis who still show normal locomotion (NW-PwMS). This would help in identifying those subjects who can benefit from early preventive exercise aimed at slowing the MS-related functional decline.

Objectives

To analyze if the assessment of walking with horizontal head turns (WHHT) through inertial sensors can discriminate between healthy subjects (HS) and NW-PwMS and between NW-PwMS subgroups. To assess if the discriminant ability of the instrumented WHHT is higher compared to clinical scores. To assess the concurrent validity of the sensor-based metrics.

Methods

In this multicenter study, 40 HS and 59 NW-PwMS [Expanded Disability Status Scale (EDSS) ≤ 2.5, disease duration ≤ 5 years] were tested. Participants executed Item-6 of the Fullerton Advanced Balance scale-short (FAB-s) wearing three inertial sensors on the trunk and ankles. The item required to horizontally turn the head at a beat of the metronome (100 bpm) while walking. Signals of the sensors were processed to compute spatiotemporal, regularity, symmetry, dynamic stability, and trunk sway metrics descriptive of WHHT.

Results

Mediolateral regularity, anteroposterior symmetry, and mediolateral stability were reduced in NW-PwMS vs. HS (p ≤ 0.001), and showed moderate discriminant ability (area under the receiver operator characteristic curve [AUC]: 0.71–0.73). AP symmetry and ML stability were reduced (p ≤ 0.026) in EDSS: 2–2.5 vs. EDSS: 0–1.5 subgroup (AUC: 0.69–0.70). The number of NW-PwMS showing at least one abnormal instrumented metric (68%) was larger (p ≤ 0.002) than the number of participants showing abnormal FAB-s-Item6 (32%) and FAB-s clinical scores (39%). EDSS: 2–2.5 subgroup included more individuals showing abnormal instrumented metrics (86%) compared to EDSS: 0–1.5 subgroup (57%). The instrumented metrics significantly correlated with FAB-s-Item6 and FAB-s scores (|Spearman's rs| ≥ 0.37, p < 0.001), thus demonstrating their concurrent validity.

Conclusion

The instrumented assessment of WHHT provided valid objective metrics that discriminated, with higher sensitivity than clinical scores, between HS and NW-PwMS and between EDSS subgroups. The method is a promising tool to complement clinical evaluation, and reveal subclinical impairments in persons who can benefit from early preventive rehabilitative interventions.
Notched Sound Alleviates Tinnitus by Reorganization Emotional Center
Authors: Huang Bixue , Wang Xianren , Wei Fanqing , Sun Qiyang , Sun Jincangjian , Liang Yue , Chen Huiting , Zhuang Huiwen , Xiong Guanxia
Published: January 2022

Background

Tinnitus is a common disease, and sound therapy is an effective method to alleviate it. Previous studies have shown that notched sound not only changes levels of cortical blood oxygen, but affects blood oxygen in specific cerebral cortical areas, such as Brodmann area 46 (BA46), which is associated with emotion. Extensive evidence has confirmed that tinnitus is closely related to emotion. Whether notched sound plays a role in regulating the emotional center is still unclear.

Methods

This study included 29 patients with newly diagnosed chronic tinnitus who were treated with notched sound. Functional near-infrared spectroscopy (fNIRS) was conducted before and after treatment to observe bilateral changes in cortical blood oxygen in the cerebral hemispheres. We compared the changes in connectivity between the two regions of interest (the superior temporal gyrus and BA46), as wells as other cortical regions before and after treatment.

Results

The results showed (1) That global connectivity between the bilateral auditory cortex of the superior temporal sulcus and the ipsilateral cortex did not change significantly between baseline and the completion of treatment, and (2) That the connectivity between channel 14 and the right superior temporal sulcus decreased after treatment. The overall connectivity between the right BA46 region and the right cortex decreased after treatment, and decreases in connectivity after treatment were specifically found for channels 10 and 14 in the right parietal lobe and channels 16, 20, 21, and 22 in the frontal lobe, while there was no significant change on the left side. There were no significant changes in the questionnaire measures of tinnitus, anxiety, or depression before and after treatment.

Conclusion

The results of the study indicate that cerebral cortex reorganization occurs in tinnitus patients after submitted to treatment with notched sound for 1 month, and that notched sound decreases the connectivity between the auditory cortex and specific brain regions.

Significance

Notched sound not only regulates the auditory center through lateral inhibition, but also alleviates tinnitus by reorganizing the emotional control center.
Effect of Age and Refractive Error on Local and Global Visual Perception in Chinese Children and Adolescents
Authors: Gan Jiahe , Wang Ningli , Li Shiming , Wang Bo , Kang Mengtian , Wei Shifei , Guo Jiyuan , Liu Luoru , Li He
Published: January 2022

Purpose

This study investigated the impact of age and myopia on visual form perception among Chinese school-age children.

Methods

This cross-sectional study included 1,074 students with a mean age of 12.1 ± 4.7 (range = 7.3–18.9) years. The mean spherical equivalence refraction (SER) of the participants was −1.45 ± 2.07 D. All participants underwent distance visual acuity (VA), refraction measurement and local and global visual form perception test including orientation, parallelism, collinearity, holes and color discrimination tasks.

Results

The reaction times of emmetropes were slower than those of myopic and high myopic groups on both local (orientation, parallelism, and collinearity) and global discrimination tasks (all p < 0.05). A reduction in reaction times was found with increasing age on both local and global discrimination tasks (all p < 0.05). Age was significantly associated with both local and global visual perception performance after adjusting for gender, visual acuity and SER (orientation, β = −0.54, p < 0.001; parallelism, β = −0.365, p < 0.001; collinearity, β = −0.28, p < 0.001; holes, β = −0.319, p < 0.001; color, β = −0.346, p < 0.001).

Conclusions

This study revealed that both local and global visual perception improve with age among Chinese children and that myopes seem to have better visual perception than emmetropes.
Behavioral Experience-Sampling Methods in Neuroimaging Studies With Movie and Narrative Stimuli
Authors: Jääskeläinen Iiro P. , Ahveninen Jyrki , Klucharev Vasily , Shestakova Anna N. , Levy Jonathan
Published: January 2022

Movies and narratives are increasingly utilized as stimuli in functional magnetic resonance imaging (fMRI), magnetoencephalography (MEG), and electroencephalography (EEG) studies. Emotional reactions of subjects, what they pay attention to, what they memorize, and their cognitive interpretations are all examples of inner experiences that can differ between subjects during watching of movies and listening to narratives inside the scanner. Here, we review literature indicating that behavioral measures of inner experiences play an integral role in this new research paradigm via guiding neuroimaging analysis. We review behavioral methods that have been developed to sample inner experiences during watching of movies and listening to narratives. We also review approaches that allow for joint analyses of the behaviorally sampled inner experiences and neuroimaging data. We suggest that building neurophenomenological frameworks holds potential for solving the interrelationships between inner experiences and their neural underpinnings. Finally, we tentatively suggest that recent developments in machine learning approaches may pave way for inferring different classes of inner experiences directly from the neuroimaging data, thus potentially complementing the behavioral self-reports.