bluuebunny/crossref_metadata_2025 huggingface dataset:
Can Chinese herbal medicine offer feasible solutions for newly diagnosed esophageal cancer patients with malnutrition? a multi-institutional real-world study
Authors: Lu Yi-Chin , Tseng Liang-Wei , Wu Chiao-En , Yang Ching-Wei , Yang Tsung-Hsien , Chen Hsing-Yu
Published: May 2024

Background

Esophageal cancer (EC) is a major cause of cancer-related mortality in Taiwan and globally. Patients with EC are highly prone to malnutrition, which adversely affects their prognosis. While Chinese herbal medicine (CHM) is commonly used alongside conventional anti-cancer treatments, its long-term impact on EC patients with malnutrition remains unclear.

Methods

This study utilized a multi-center cohort from the Chang Gung Research Database, focusing on the long-term outcomes of CHM in EC patients with malnutrition between 1 January 2001, and 31 December 2018. Patients were monitored for up to 5 years or until death. Overall survival (OS) rates were calculated using the Kaplan-Meier method. Overlap weighting and landmark analysis were employed to address confounding and immortal time biases. Additionally, the study analyzed prescription data using a CHM network to identify key CHMs for EC with malnutrition, and potential molecular pathways were investigated using the Reactome database.

Results

EC patients with malnutrition who used CHM had a higher 5-year OS compared with nonusers (22.5% vs. 9% without overlap weighting; 24.3% vs. 13.3% with overlap weighting; log-rank test: p = 0.006 and 0.016, respectively). The median OS of CHM users was significantly longer than that of nonusers (19.8 vs. 12.9 months, respectively). Hazard ratio (HR) analysis showed a 31% reduction in all-cause mortality risk for CHM users compared with nonusers (HR: 0.69, 95% confidence interval: 0.50–0.94, p = 0.019). We also examined 665 prescriptions involving 306 CHM, with Hedyotis diffusa Willd. exhibiting the highest frequency of use. A CHM network was created to determine the primary CHMs and their combinations. The identified CHMs were associated with the regulation of immune and metabolic pathways, particularly in areas related to immune modulation, anti-cancer cachexia, promotion of digestion, and anti-tumor activity.

Conclusion

The results of this study suggest a correlation between CHM use and improved clinical outcomes in EC patients with malnutrition. The analysis identified core CHMs and combinations of formulations that play a crucial role in immunomodulation and metabolic regulation. These findings lay the groundwork for more extensive research on the use of CHM for the management of malnutrition in patients with EC.
The influence of drug-induced metabolic enzyme activity inhibition and CYP3A4 gene polymorphism on aumolertinib metabolism
Authors: Ye Feng , Ni Jinhuan , Li Xinyue , Wang Jing , Luo Jianchao , Wang Shiyu , Xu Xiaoyu , Zhong Yunshan , Qian Jianchang , Xiao Zhongxiang
Published: May 2024

The purpose of this study is to clarify the drug interaction profile of aumolertinib, and the influence of CYP3A4 genetic polymorphism on aumolertinib metabolic characteristics. Through microsomal enzyme reactions, we screened 153 drugs and identified 15 that significantly inhibited the metabolism of aumolertinib. Among them, telmisartan and carvedilol exhibited potent inhibitory activities in rat liver microsomes (RLM) and human liver microsomes (HLM). In vivo, the pharmacokinetic parameters of aumolertinib, including AUC and Cmax, were significantly altered when co-administered with carvedilol, with a notable decrease in the clearance rate CLz/F. Interestingly, the pharmacokinetic parameters of the metabolite HAS-719 exhibited a similar trend as aumolertinib when co-administered. Mechanistically, both telmisartan and carvedilol exhibited a mixed-type inhibition on the metabolism of aumolertinib. Additionally, we used a baculovirus-insect cell expression system to prepare 24 recombinant CYP3A4 microsomes and obtained enzymatic kinetic parameters using aumolertinib as a substrate. Enzyme kinetic studies obtained the kinetic parameters of various CYP3A4 variant-mediated metabolism of aumolertinib. Based on the relative clearance rates, CYP3A4.4, 5, 7, 8, 9, 12, 13, 14, 17, 18, 19, 23, 24, 33, and 34 showed significantly lower clearance rates compared to the wild-type. Among the different CYP3A4 variants, the inhibitory potency of telmisartan and carvedilol on the metabolism of aumolertinib also varied. The IC50 values of telmisartan and carvedilol in CYP3A4.1 were 6.68 ± 1.76 μM and 0.60 ± 0.25 μM, respectively, whereas in CYP3A4.12, the IC50 exceeded 100 μM. Finally, we utilized adeno-associated virus to achieve liver-specific high expression of CYP3A4*1 and CYP3A4*12. In the group with high expression of the less active CYP3A4*12, the magnitude of the drug-drug interaction was significantly attenuated. In conclusion, CYP3A4 genetic polymorphism not only influences the pharmacokinetic characteristics of aumolertinib, but also the inhibitory potency of telmisartan and carvedilol on it.
Simulation of thermal field in mass concrete with cooling pipes based on the isogeometric analysis method
Authors: Li Qingwen , Chen Guorong , Zhu Fuxian
Published: May 2024

As the water pipe cooling system is widely applied to controlling temperature in mass concrete structures, the precise simulation of the temperature field in mass concrete with cooling pipes embedded is meaningful. This paper presents an isogeometric analysis (IGA) with NURBS for heat transfer in mass concrete with consideration of the cooling pipe. The proposed method not only achieves the same level of accuracy with fewer nodes but also eliminates the time-consuming process of mesh in the traditional FEM. The coarsest parameter space which depicts small pipe and large concrete precisely is constructed to create an efficient model for numerical computation. In addition, the unique k-refinement in IGA is supposed to be the most appropriate encryption mechanism, and the knot insertion vector for effective refinement is calculated by considering the characteristics of temperature gradient distribution around the cooling pipes. In addition, a different calculation parameter has been discussed to show the stability and flexibility of the IGA. The obtained numerical results demonstrate the accuracy and efficiency of the proposed scheme in the simulation of transient temperature fields in concrete structures with cooling systems.
Plastid phylogenomics clarifies broad-level relationships in Bulbophyllum (Orchidaceae) and provides insights into range evolution of Australasian section Adelopetalum
Authors: Simpson Lalita , Clements Mark A. , Orel Harvey K. , Crayn Darren M. , Nargar Katharina
Published: May 2024

The hyperdiverse orchid genus Bulbophyllum is the second largest genus of flowering plants and exhibits a pantropical distribution with a center of diversity in tropical Asia. The only Bulbophyllum section with a center of diversity in Australasia is sect. Adelopetalum. However, the phylogenetic placement, interspecific relationships, and spatio-temporal evolution of this section remain largely unclear. To infer broad-level relationships within Bulbophyllum, and interspecific relationships within sect. Adelopetalum, a genome skimming dataset was generated for 89 samples, which yielded 70 plastid coding regions and a nuclear ribosomal DNA cistron. For 18 additional samples, Sanger data from two plastid loci (matK and ycf1) and nuclear ITS were added using a supermatrix approach. The study provided new insights into broad-level relationships in Bulbophyllum, including phylogenetic evidence for the non-monophyly of sections Beccariana, Brachyantha, Brachypus, Cirrhopetaloides, Cirrhopetalum, Desmosanthes, Minutissima, Oxysepala, Polymeres, and Sestochilos. Section Adelopetalum and sect. Minutissima s.s. formed a highly supported clade that was resolved as a sister group to the remainder of the genus. Divergence time estimations based on a relaxed molecular clock model placed the origin of Bulbophyllum in the Early Oligocene (ca. 33.2 Ma) and sect. Adelopetalum in the Late Oligocene (ca. 23.6 Ma). Ancestral range estimations based on a BAYAREALIKE model identified the Australian continent as the ancestral area of the sect. Adelopetalum. The section underwent crown diversification from the mid-Miocene to the late Pleistocene, predominantly in continental Australia. At least two independent long-distance dispersal events were inferred eastward from the Australian continent to New Zealand and to New Caledonia from the early Pliocene onwards, likely mediated by predominantly westerly winds of the Southern hemisphere. Retraction and fragmentation of the eastern Australian rainforests from the early Miocene onwards are likely drivers of lineage divergence within sect. Adelopetalum facilitating allopatric speciation.
Observing prosociality and talent: the emotional characteristics and behavioral outcomes of elevation and admiration in 6.5- to 8.5-year-old children
Authors: Gibhardt Sina , Hepach Robert , Henderson Annette M. E.
Published: May 2024

Helping and seeing others being helped elicits positive emotions in young children but little is known about the nature of these emotions, especially in middle childhood. Here we examined the specific emotional characteristics and behavioral outcomes of two closely related other-praising moral emotions: elevation and admiration. We exposed 182 6.5- to 8.5-year-old children living in New Zealand, to an elevation- and admiration-inducing video clip. Afterwards children’s emotion experiences and prosocial behaviour was measured. Findings revealed higher levels of happiness, care, and warmth after seeing prosociality in others (elevation condition) and higher levels of upliftment after seeing talent in others (admiration condition). We found no differences in prosocial behavior between the elevation and admiration conditions. This is the first study to assess elevation in childhood and offers a novel paradigm to investigate the role of moral emotions as potential motivators underlying helping.
Empathy in schizophrenia: neural alterations during emotion recognition and affective sharing
Authors: Knobloch Simon , Leiding Delia , Wagels Lisa , Regenbogen Christina , Kellermann Thilo , Mathiak Klaus , Schneider Frank , Derntl Birgit , Habel Ute
Published: May 2024

Introduction

Deficits in emotion recognition and processing are characteristic for patients with schizophrenia [SCZ].

Methods

We targeted both emotion recognition and affective sharing, one in static and one in dynamic facial stimuli, during functional magnetic resonance imaging [fMRI] in 22 SCZ patients and 22 matched healthy controls [HC]. Current symptomatology and cognitive deficits were assessed as potential influencing factors.

Results

Behaviorally, patients only showed a prolonged response time in age-discrimination trials. For emotion-processing trials, patients showed a difference in neural response, without an observable behavioral correlate. During emotion and age recognition in static stimuli, a reduced activation of the bilateral anterior cingulate cortex [ACC] and the right anterior insula [AI] emerged. In the affective sharing task, patients showed a reduced activation in the left and right caudate nucleus, right AI and inferior frontal gyrus [IFG], right cerebellum, and left thalamus, key areas of empathy.

Discussion

We conclude that patients have deficits in complex visual information processing regardless of emotional content on a behavioral level and that these deficits coincide with aberrant neural activation patterns in emotion processing networks. The right AI as an integrator of these networks plays a key role in these aberrant neural activation patterns and, thus, is a promising candidate area for neurofeedback approaches.
Knowledge, attitude, and practice toward ultrasound screening for breast cancer among women
Authors: Liu Shaozhong , Zheng Shukai , Qin Mengzhen , Xie Yifeng , Yang Kun , Liu Xiaozhen
Published: May 2024

Background

Several obstacles can hinder breast cancer screening. This study aimed to investigate the knowledge, attitude, and practice (KAP) toward ultrasound screening for breast cancer in women.

Methods

This cross-sectional study recruited women who visited the breast specialist clinic of Zhongshan City People’s Hospital (a tertiary hospital) between August 2022 and April 2023 through convenience sampling. KAP scores ≥70% were considered adequate.

Results

This study enrolled 501 participants. The mean knowledge, attitude, and practice levels were 8.56 ± 1.81/12 (possible range 0–12, 71.33%), 29.80 ± 2.71 (possible range 8–40, 74.50%), and 32.04 ± 3.09 (possible range 8–40, 80.10%). Senior high school education (vs. junior high school and below, coefficient = 1.531, 95%CI: 1.013–2.312, p = 0.044), bachelor’s education and above (vs. junior high school and below, coefficient = 5.315, 95%CI: 3.546–7.966, p < 0.001), housewife or unemployed (vs. employed, coefficient = 0.671, 95%CI: 0.466–0.966, p = 0.032), and a history of breast ultrasound (vs. no, coefficient = 1.466, 95%CI: 1.121–1.917, p = 0.005) were independently and positively associated with knowledge. Knowledge (coefficient = 1.303, 95%CI: 1.100–1.544, p = 0.002) and monthly income >10,000 (vs. <5,000, coefficient = 4.364, 95%CI: 1.738–10.956, p = 0.002) were independently and positively associated with attitude. Only attitude (coefficient = 1.212, 95%CI: 1.096–1.340, p < 0.001) was independently and positively associated with the practice. A structural equation modeling (SEM) analysis was used to estimate causality among KAP dimensions, showing that knowledge directly influenced attitude (β = −1.090, p = 0.015), knowledge did not directly influence practice (β = −0.117, p = 0.681) but had an indirect influence (β = 0.826, p = 0.028), and attitude directly influenced practice (β = −0.757, p = 0.016).

Conclusion

Women in Zhongshan City had good knowledge, favorable attitudes, and active practice toward breast ultrasound screening for breast cancer. Women’s characteristics associated with a poorer KAP were identified, allowing for more targeted interventions.
Mapping social movements momentum: unveiling networks in the movement for the right to abortion in Mexico
Authors: López Martínez Cesángari , Sambo Allison , Medina González Diana
Published: May 2024

In this case study, we propose a network analysis approach to map social movements through an intersectional feminist lens. We aim to gain a more in-depth understanding of movements' network structures to examine the roles and relationships of movement actors, the flow of resources between them, and potential areas of collaboration and conflict. By incorporating Social Network Analysis (SNA) techniques and visually mapping dynamics within social movements, this approach can assess the significance of small actors in creating change and stresses the need for their perspectives to be heard. Furthermore, our methodology adopts a feminist intersectional framework that recognizes the role of different identities in shaping the movement and its actors. To demonstrate the practical application of this approach, we examined the movement for the right to abortion in Mexico between September and December 2021. Our multi-step process included (1) designing a survey tool adapted to the context of the movement, (2) collecting and analyzing survey responses on movement actors' relationships and interactions, including their priorities, activities, needs, risks, and challenges, (3) visualizing the network using SNA techniques that account for the complexity and diversity of the movement, (4) interpreting the findings through insights collected in semi-structured interviews and validation meetings with key movement actors, and (5) implementing safety and security guidelines to safeguard the identity of individuals whose activities could put them at risk of suffering institutional, emotional, and physical violence. Our case study offers valuable insights that not only encourage the integration of feminist and intersectional perspectives into data collection processes but also provide a roadmap for accompanying social movements and supporting meaningful and contextually responsive activities.
Efficacy of a remote virtual reality and EEG enabled psychotherapy system for the treatment of depressive symptoms
Authors: Tacca Christopher , Kerr Barbara A. , McLamb Christopher , Ridgway Kaylie Lyons , Friis Elizabeth A.
Published: May 2024

More than 40% of the U.S. population have experienced mental health disorders since the COVID-19 pandemic. 40% of this group received no treatment for their mental illness. Barriers to treatment include stigma, prohibitive cost, and a belief that treatment is inaccessible, particularly in isolated or rural communities. A novel remote, EEG-enhanced VR psychotherapy system was assessed for its presence and restorativeness, and therapeutic efficacy in improving mood with a single session positive solution-focused session. Thirty adults experiencing depressive symptoms were randomly assigned to either a single session Positive Solutions Focused counseling treatment via Zoom videoconferencing, or the EEG enabled VR psychotherapy system. Participants rated the environment in the VR-EEG therapy as more restorative than Zoom counseling,t= 2.928,p< .004, Cohen’s d = .259, and comparable to the Zoom session in presence. The VR-EEG system performed comparably to Zoom online counseling in clients’ session ratings of depth and smoothness and client reactions, positivity, and arousal. For a treatment to be considered empirically supported, and therefore valid for use in psychotherapy, it must have equal or greater efficacy than a standard treatment or format. VR-EEG, therefore, has promise as a positive, solution-focused, brief therapy for isolated clients with depressive symptoms.
Transforming food systems in the Global South: a radical approach
Authors: Suarez Andres , Ume Chukwuma
Published: May 2024

Sustainability within food systems (FS) transcends approaches that only consider FS transformation via changing agricultural practices or consumption patterns. The essence lies in addressing the root causes of current unsustainable FS and their associated social and environmental ramifications. This paper aims to outline the solutions needed to revamp these challenges, by paying special attention to the state-capital nexus in the context of the FS’global core-periphery dialectics. Thereby, we embrace radical political agroecology as being essential in promoting sustainability within the FS, especially in the Global South. Agroecology is proposed as the strategy to address the food system’s complexity in terms of the social, environmental, and economic embeddedness. We conclude with potential solutions that contribute to the pathway for FS sustainability.
Comprehensive transcriptomic and metabolomic analysis of porcine intestinal epithelial cells after PDCoV infection
Authors: Wang Guangzheng , Cao Yanan , Xu Chao , Zhang Shuoshuo , Huang Yanjie , Zhang Shuai , Bao Wenbin
Published: May 2024

Introduction

Porcine deltacoronavirus (PDCoV), an emerging swine enteropathogenic coronavirus with worldwide distribution, mainly infects newborn piglets with severe diarrhea, vomiting, dehydration, and even death, causing huge economic losses to the pig industry. However, the underlying pathogenic mechanisms of PDCoV infection and the effects of PDCoV infection on host transcripts and metabolites remain incompletely understood.

Methods

This study investigated a combined transcriptomic and metabolomic analysis of porcine intestinal epithelial cells (IPEC-J2) following PDCoV infection by LC/MS and RNA-seq techniques. A total of 1,401 differentially expressed genes and 254 differentially accumulated metabolites were detected in the comparison group of PDCoV-infected vs. mock-infected.

Results and discussion

We found that PDCoV infection regulates gene sets associated with multiple signaling pathways, including the neuroactive ligand-receptor interaction, cytokine-cytokine receptor interaction, MAPK signaling pathway, chemokine signaling pathway, ras signaling pathway and so on. Besides, the metabolomic results showed that biosynthesis of cofactors, nucleotide metabolism, protein digestion and absorption, and biosynthesis of amino acid were involved in PDCoV infection. Moreover, integrated transcriptomics and metabolomics analyses revealed the involvement of ferroptosis in PDCoV infection, and exogenous addition of the ferroptosis activator erastin significantly inhibited PDCoV replication. Overall, these unique transcriptional and metabolic reprogramming features may provide a better understanding of PDCoV-infected IPEC-J2 cells and potential targets for antiviral treatment.
Genetic characterization and evidence for multiple reassortments of rotavirus A G3P[3] in dogs and cats in Thailand
Authors: Chamsai Ekkapat , Charoenkul Kamonpan , Udom Kitikhun , Jairak Waleemas , Chaiyawong Supassama , Amonsin Alongkorn
Published: May 2024

Rotavirus A (RVA) causes gastroenteritis in humans and animals. The zoonotic potential of RVA has been reported and raises major concerns, especially in animal-human interface settings. The study aimed to characterize and investigate the genetic diversity among RVAs in dogs and cats in Thailand. We collected 572 rectal swab samples from dogs and cats in Bangkok animal hospitals from January 2020 to June 2021. The one-step RT-PCR assay detected RVAs in 1.92% (11/572) of the samples, with 2.75% (8/290) in dogs and 1.06% (3/282) in cats. Two canine RVA and one feline RVA were subjected to whole genome sequencing. Our results showed that all three viruses were identified as RVA genotype G3P[3]. The genetic constellation of RVAs is unique for different species. For canine RVAs is G3-P [3]-I3-R3-C3-M3-A9-N2-T3-E3-H6, while Feline RVA is G3-P [3]-I8-R3-C3-M3-A9-N3-T3-E3-H6. Notably, both canine and feline RVAs contained the AU-1 genetic constellation with multiple reassortments. The results of phylogenetic, genetic, and bootscan analyses showed that canine RVAs may have reassorted from dog, human, and cat RVAs. While feline RVA was closely related to RVAs in humans, bats, and simians. This study provided genetic characteristics and diversity of RVAs in dogs and cats and suggested possible multiple reassortments, suggesting the zoonotic potential of the viruses. Thus, public health awareness should be raised regarding the zoonotic potential of RVAs in dogs and cats. Further studies on RVAs on a larger scale in dogs and cats in Thailand are needed.
Elements of Weight Management Among Pre-Kidney Transplant Candidates: The Patient Perspective
Authors: Chirban Ariana , del Valle Diana D. , Coe Taylor , Cote Maria P. , Chen Maggie , Cataldo Jennie , Elias Nahel , Shah Anushi , Dageforde Leigh Anne
Published: May 2024

Obesity and related comorbidities heighten risks for complications in kidney transplant settings. While pre-transplant patients often have access to nutrition counseling and health support, literature is limited on patients' perceptions of weight and motivation to lose weight prior to transplantation. We conducted a survey among ≥18-year-old patients on the kidney transplant waitlist at a single center. Questions addressed weight perception, motivation for weight loss, available resources, and engagement in physical activity. Medical records provided demographic and clinical data. Statistical tests analyzed quantitative data, while free-text responses were thematically grouped and described. Of 1055 patients, 291 responded and were matched with demographic data. Perceived weight changes correlated with actual changes in body mass index (BMI) (<24.9) were more receptive to weight center resources (<30 kg/m2) are most interested in weight loss resources and demonstrate motivation. Furthermore, pre-transplant nutrition counseling correlates with healthier behaviors. Integrating patients’ perspectives enhances pre-transplant protocols by encouraging active involvement in health decisions.
Explainable AI Frameworks: Navigating the Present Challenges and Unveiling Innovative Applications
Authors: Sharma Neeraj Anand , Chand Rishal Ravikesh , Buksh Zain , Ali A. B. M. Shawkat , Hanif Ambreen , Beheshti Amin
Published: May 2024

This study delves into the realm of Explainable Artificial Intelligence (XAI) frameworks, aiming to empower researchers and practitioners with a deeper understanding of these tools. We establish a comprehensive knowledge base by classifying and analyzing prominent XAI solutions based on key attributes like explanation type, model dependence, and use cases. This resource equips users to navigate the diverse XAI landscape and select the most suitable framework for their specific needs. Furthermore, the study proposes a novel framework called XAIE (eXplainable AI Evaluator) for informed decision-making in XAI adoption. This framework empowers users to assess different XAI options based on their application context objectively. This will lead to more responsible AI development by fostering transparency and trust. Finally, the research identifies the limitations and challenges associated with the existing XAI frameworks, paving the way for future advancements. By highlighting these areas, the study guides researchers and developers in enhancing the capabilities of Explainable AI.
An Engine Deterioration Model for Predicting Fuel Consumption Impact in a Regional Aircraft
Authors: Gurrola Arrieta Manuel de Jesús , Botez Ruxandra M. , Lasne Axel
Published: May 2024

A deterioration cycle model is presented, designed to consider the turbomachinery efficiency losses that are expected during real engine in-service operations. The cycle model was developed using information from practical experience found in the literature to account for both short- and long-term deterioration effects; the former occurring during the first flight cycles, the latter due to regular in-service operation. This paper highlights the importance of analyzing the inter-turbine temperature margin (ITTM) to track engine deterioration to determine the extent of an in-service engine life. The proposed model was used to assess the ITTM and fuel consumption impact in the CRJ-700 regional aircraft (powered by two CF34-8C5B1 engines) for three representative missions: short (0.4 h), average (1.4 h), and long (2.5 h), considering different levels of engine deterioration, from the new engine level up to fully deteriorated. The fuel consumption at the new engine level (zero deterioration) was validated against a real-time engine model embedded in a Level-D flight simulator, the so-called Virtual Research Flight Simulator located at the Laboratory of Applied Research in Active Control, Avionics, and AeroServoElasticity. The errors found in this validation for the trip mission fuel consumption in the short, average, and long missions were −3.6, +0.9, and +0.6%, respectively. The cycle model predictions suggest the ITTM for a new engine is 55.2 °C, whereas for a fully deteriorated engine, it is 26.4 °C. Finally, in terms of fuel consumption, the results presented here show that an average CF34-8C5B1 engine shows an increase in the cumulative fuel consumption of 2.25% during its life in service, which translates to a 4.5% impact in aircraft fuel consumption.
Tailoring 3D Star-Shaped Auxetic Structures for Enhanced Mechanical Performance
Authors: Wang Yulong , Alsaleh Naser A. , Djuansjah Joy , Hassanin Hany , El-Sayed Mahmoud Ahmed , Essa Khamis
Published: May 2024

Auxetic lattice structures are three-dimensionally designed intricately repeating units with multifunctionality in three-dimensional space, especially with the emergence of additive manufacturing (AM) technologies. In aerospace applications, these structures have potential for use in high-performance lightweight components, contributing to enhanced efficiency. This paper investigates the design, numerical simulation, manufacturing, and testing of three-dimensional (3D) star-shaped lattice structures with tailored mechanical properties. Finite element analysis (FEA) was employed to examine the effect of a lattice unit’s vertex angle and strut diameter on the lattice structure’s Poisson’s ratio and effective elastic modulus. The strut diameter was altered from 0.2 to 1 mm, while the star-shaped vertex angle was adjusted from 15 to 90 degrees. Laser powder bed fusion (LPBF), an AM technique, was employed to experimentally fabricate 3D star-shaped honeycomb structures made of Ti6Al4V alloy, which were then subjected to compression testing to verify the modelling results. The effective elastic modulus was shown to decrease when increasing the vertex angle or decreasing the strut diameter, while the Poisson’s ratio had a complex behaviour depending on the geometrical characteristics of the structure. By tailoring the unit vertex angle and strut diameter, the printed structures exhibited negative, zero, and positive Poisson’s ratios, making them applicable across a wide range of aerospace components such as impact absorption systems, aircraft wings, fuselage sections, landing gear, and engine mounts. This optimization will support the growing demand for lightweight structures across the aerospace sector.
Damping Optimization Method of Combine Harvester Frame Undergoing Multi-Source Excitation
Authors: Wang Bangzhui , Chen Shuren , Wang Guoqiang , Tang Zhong , Ding Hantao
Published: May 2024

The complex mechanical system of a rice combine harvester not only has various excitation sources, but also, the vibration transmission path between each working device and the vibration contribution characteristics to the frame are not clear, so it is difficult to perform a reduction vibration design for the sharp vibration of the rice combine harvester frame. Therefore, based on the comparison and improvement of multiple classical transfer path analysis methods, this paper analyzed the vibration transfer characteristics and transfer characteristics of each harvester by the discrete time matrix method and operating path method. In the Experimental section, through the vibration characteristic experiment firstly, this paper obtained the power spectrum variation and the most needed optimized path in the transmission path of each device under each operating condition. Secondly, through frame simulation analysis under the exciting force, we obtained the vibration damping areas that needs to be optimized. Finally, the damping optimization experiment connected with the vibration characteristic experiment, and the excitation force simulation analysis was performed. The results of the damping optimization experiment displayed that the maximum change value of the vibration acceleration of the cutting table decreased from 7.862 m·s−2 to 3.522 m·s−2, decreasing by 55.2%, and the peak amplitude of the multipoint test in the cab was 5.4, 5.3, 1.7 and 2.0 μm, respectively, which was significantly reduced, so the optimization effect was significant. This study provides theoretical support for the vibration reduction optimization of a rice combine harvester frame.
Nitrogen Fertiliser Effects on Grain Anthocyanin and γ-Oryzanol Biosynthesis in Black Rice
Authors: Thapa Manisha , Liu Lei , Barkla Bronwyn J. , Kretzschmar Tobias , Rogiers Suzy Y. , Rose Terry J.
Published: May 2024

Accumulation of phytochemicals in vegetative tissue under nitrogen (N) stress as an adaptive strategy has been investigated in various crops, but the effect of applied N on grain phytochemicals is poorly understood. This study investigated the effect of applied N on the biosynthesis and accumulation of rice (Oryza sativa L.) grain anthocyanin and γ-oryzanol under different ultraviolet-B (UV-B) conditions in a controlled pot trial using two distinct black rice genotypes. The response of grain anthocyanin and γ-oryzanol content to applied N was genotype-dependent but was not altered by UV-B conditions. Applied N increased grain anthocyanin and decreased γ-oryzanol content in genotype SCU212 but had no significant effect in genotype SCU254. The expression of the OsKala3 regulatory gene was significantly upregulated in response to applied N in SCU212, while the expressions of OsKala4 and OsTTG1 were unchanged. The expression of all three regulatory genes was not significantly affected in SCU254 with applied N. Key anthocyanin biosynthesis genes were upregulated in grain by N application, which indicates that the common increase in anthocyanin in vegetative tissues under N deprivation does not hold true for reproductive tissues. Hence, any future approach to target higher content of these key phytochemicals in grains should be genotype-focused.
MOLO-SLAM: A Semantic SLAM for Accurate Removal of Dynamic Objects in Agricultural Environments
Authors: Lv Jinhong , Yao Beihuo , Guo Haijun , Gao Changlun , Wu Weibin , Li Junlin , Sun Shunli , Luo Qing
Published: May 2024

Visual simultaneous localization and mapping (VSLAM) is a foundational technology that enables robots to achieve fully autonomous locomotion, exploration, inspection, and more within complex environments. Its applicability also extends significantly to agricultural settings. While numerous impressive VSLAM systems have emerged, a majority of them rely on static world assumptions. This reliance constrains their use in real dynamic scenarios and leads to increased instability when applied to agricultural contexts. To address the problem of detecting and eliminating slow dynamic objects in outdoor forest and tea garden agricultural scenarios, this paper presents a dynamic VSLAM innovation called MOLO-SLAM (mask ORB label optimization SLAM). MOLO-SLAM merges the ORBSLAM2 framework with the Mask-RCNN instance segmentation network, utilizing masks and bounding boxes to enhance the accuracy and cleanliness of 3D point clouds. Additionally, we used the BundleFusion reconstruction algorithm for 3D mesh model reconstruction. By comparing our algorithm with various dynamic VSLAM algorithms on the TUM and KITTI datasets, the results demonstrate significant improvements, with enhancements of up to 97.72%, 98.51%, and 28.07% relative to the original ORBSLAM2 on the three datasets. This showcases the outstanding advantages of our algorithm.
Management of Rust in Wheat Using IPM Principles and Alternative Products
Authors: Jørgensen Lise Nistrup , Matzen Niels , Leitzke Rebekka , Thomas Jane E. , O’Driscoll Aoife , Klocke Bettina , Maumene Claude , Lindell Ida , Wahlquist Kerstin , Zemeca Līga , Apesteguia Marcos Barberena , Randazzo Biango , Slikova Svetlana , Holdgate Sarah
Published: May 2024

Overall, there is a major wish that European farmers implement integrated pest management (IPM), particularly to reduce dependence on pesticides. In the European Rustwatch project, partners conducted nineteen trials across nine different countries during 2020 and 2021 to investigate different IPM strategies, focusing on controlling rust diseases in winter wheat. The trials included the use of varieties with contrasting levels of resistance, variety mixtures, reduced fungicide rates, thresholds, and Decision Support Systems (DSSs), and testing alternative products to fungicides. Sixteen trials developed yellow rust (Puccinia striiformis f. sp. tritici) infections, and six trials developed brown rust (Puccinia triticina) infections. Resistant varieties proved highly effective in keeping down yellow rust infection, and variety mixtures also effectively reduced infection levels and stabilized yields. Rust was fully controlled using 25% of standard fungicide rates, even under high disease pressure. Using DSSs provided sufficient control of rust diseases and resulted in competitive net economic returns due to fewer fungicide applications. The alternative products tested included two biological control agents and four alternative chemistries, which all gave inferior and insufficient control against rust compared with chemical fungicides. The trial work demonstrated that there are good and reliable options for including IPM into disease control in wheat.
Micropropagation of Al-Taif Rose: Effects of Medium Constituents and Light on In Vitro Rooting and Acclimatization
Authors: Al-Ali Ali Mohsen , Dewir Yaser Hassan , Al-Obeed Rashid Sultan
Published: May 2024

Micropropagation facilitates the rapid production of roses. Nevertheless, in vitro rhizogenesis of essential oil roses has presented significant challenges, primarily attributed to low rates of rooting and poorer acclimatization compared to ornamental rose varieties. This study reports the optimization of in vitro rooting of Al-Taif rose (Rosa damascena f. trigintipetala (Diek) R. Keller) microshoots with the aim of increasing survival rate during acclimatization. We also investigated the effects of various parameters, including type and concentration of auxin (i.e., 2,4-Dichlorophenoxyacetic acid (2,4-D), indole acetic acid (IAA), indole butyric acid (IBA), and naphthaleneacetic acid (NAA) at concentrations of 0, 0.05, 0.1, 0.2, and 0.4 mg/L), salt strength (i.e., full- and half-strength Murashige and Skoog (MS) medium), sucrose concentration (i.e., 20, 30, 40, 60, and 80 g/L), light spectra (a 2:1 or 1:2 blue/red spectral ratio, cool or warm white light at a 1:1 ratio, and fluorescent light), light intensity (photosynthetic photon flux density (PPFD) values of 25, 50, and 100 µmol·m−2·s−1), and activated charcoal (i.e., 0 and 0.5 g/L) on the rooting and growth of in vitro regenerated Al-Taif rose axillary shoots. We found that half-strength MS medium supplemented with 0.2 mg/L NAA, 80 g/L sucrose, 0.5 g/L activated charcoal, and 50 μmol·m−2·s−1 PPFD were the optimal conditions for 100% induction of adventitious roots. Next, micropropagated Al-Taif rose plantlets were successfully transferred to a potting medium containing perlite/peatmoss (in a 1:1 ratio). We found that 98% of plants survived ex vitro conditions. The genetic fidelity of micropropagated Al-Taif rose clones along with their mother plant was tested using the inter-simple sequence repeats (ISSR) molecular marker. The genetic similarity between the micropropagated plantlets and the mother plant of Al-Taif rose plants was 98.8%, revealing high uniformity and true-to-type regenerated plants. These findings may therefore contribute toward the commercial micropropagation of Al-Taif roses.
Optimizing Irrigation and Fertilization to Simultaneously Improve Potato Tuber Yield, Water and Fertilizer Use Efficiency and Net Income in Northwest China
Authors: Zhang Fan , Chen Mengru , Zheng Yu , Xie Yunxia , Xing Yingying
Published: May 2024

Irrigation, fertilization, and variety are important factors affecting potato production in northwest China. Field experiments (2021 and 2022) were performed to investigate the effects of irrigation and fertilization on the plant growth and soil microbial population of different potato varieties. Three irrigation levels were used, i.e., 100% ETc (W1), 80% ETc (W2), and 60% ETc (W3), with ETc standing for crop evapotranspiration. Three fertilization levels were used (N-P-K), i.e., 240-120-300 kg ha−1 (F1), 180-90-225 kg ha−1 (F2), and 120-60-150 kg ha−1 (F3). Three variety types were used, i.e., Feiurita (V1), Longshu 7 (V2), and Qingshu 9 (V3). These factors significantly influenced tuber yield (TY), net income (NI), and water productivity (WP). TY, NI, WP, total nitrogen accumulation (TNA), and nitrogen use efficiency (NUE) peaked at F2. Fertilization significantly impacted soil bacteria quantity (SBQ), fungi quantity (SFQ), and actinomycetes quantity (SAQ). TY, NI, SBQ, SFQ, and SAQ were highest at W2. Soil microbial population was strongly correlated with TY, NI, WP, TNA, and NUE. Comprehensively, this study suggests that irrigation that is varied from 248 to 266 mm, and fertilization (N-P-K) that is varied from 149.09-74.55-186.36 to 212.73-106.36-265.91 kg ha−1 can promote the potato industry’s sustainable development and provide important references for the optimal field management of potato cultivation in northwest China.
Effect of Biochar on Apple Yield and Quality in Aged Apple Orchards on the Loess Plateau (China)
Authors: Li Wenzheng , Gao Jianen , Zhou Shuang , Zhou Fanfan
Published: May 2024

Apples are not only a foodstuff, but also a raw material for many industrial production fields, and the market demand is constantly increasing. The Loess Plateau is one of the world’s largest apple-producing areas, with about 85% are aged orchards (more than 20 years old), facing problems such as poor soil water retention, degradation of tree strength, and declining yield and quality, etc., so do aged orchards in other regions of the world, and study on improving quality and increasing yield of aged orchards is of great significance to the sustainable development of the apple industry. Here, 6 treatments (2, 4, 6, 8, 10 and 12 kg/plant) were designed in the experiment to study the effects of biochar on yield and quality in aged apple orchards. The study showed that: biochar could improve soil water retention, but caused the soil alkalization; biochar could improve apple quality and increase yield, T3 and T4 were of better quality, and T3 had the highest yield. Comprehensive analysis, T3 is the optimal scheme. The results not only provide a reference for aged orchards worldwide, but also have great significance for the sustainable development of the apple industry.
Assessment of the Characteristics of Corncobs Used for Energy Needs
Authors: Dorofėjūtė Tautvydė , Paulikienė Simona , Ūksas Tomas , Zvicevičius Egidijus , Žiūra Kęstutis , Lekavičienė Kristina
Published: May 2024

Due to environmental pollution and global warming, the use of renewable energy sources such as agricultural residues is gaining attention. Corn is the main source of food in many countries, and after harvesting it, the agricultural and industrial sectors generate significant amounts of residues. This study focused on corncob processing, its preparation, and the evaluation of its characteristics for energy needs. There was no significant difference between drying with active ventilation in the dryer and outdoor drying conditions. The adequate moisture content of corncob pellets is 12.39% at a compression ratio of 3.43 ± 0.011 and a maximum pellet density of 1012.96 ± 3.35 kg m−3. The variation in pellet density at a given adequate moisture content is at least 0.78%. The compression ratio of the pellets compacted on the horizontal matrix granulator is 9.75% higher than that of the pellets produced on the laboratory automatic press. The corncob’s lower calorific value was 17.35 ± 0.14 MJ kg−1, and the ash content was 1.78 ± 0.24%. The produced pellets are strong enough and can be used for combustion. This research may help to better understand the properties of corncobs and their energy potential.
Zoo and Aquarium Animal Welfare, Ethics, and Behavior
Authors: Miller David S. , Dudzinski Kathleen M. , Anthony Raymond , Hill Heather Manitzas , Stafford Grey
Published: May 2024

This Special Issue of Animals was launched to promote the discussion of how animal welfare can be best addressed in zoos and aquariums while accommodating conservation needs, as well as how further improvements can be made in a similar vein to how medical and scientific research has advanced human health and welfare [...]
Spent Material Extractives from Hemp Hydrodistillation as an Underexplored Source of Antimicrobial Cannabinoids
Authors: Luca Simon Vlad , Wojtanowski Krzysztof , Korona-Głowniak Izabela , Skalicka-Woźniak Krystyna , Minceva Mirjana , Trifan Adriana
Published: May 2024

Hemp (Cannabis sativa L.) has been used for millennia as a rich source of food and fibers, whereas hemp flowers have only recently gained an increased market interest due to the presence of cannabinoids and volatile terpenes. Currently, the hemp flower processing industry predominantly focuses on either cannabinoid or terpene extraction. In an attempt to maximize the valorization of hemp flowers, the current study aimed to evaluate the phytochemical composition and antimicrobial properties of several extracts obtained from post-distillation by-products (e.g., spent material, residual distillation water) in comparison to the essential oil and total extract obtained from unprocessed hemp flowers. A terpene analysis of the essential oil revealed 14 monoterpenes and 35 sesquiterpenes. The cannabinoid profiling of extracts showed seven acidic precursors and 14 neutral derivatives, with cannabidiol (CBD) reaching the highest concentration (up to 16 wt.%) in the spent material extract. The antimicrobial assessment of hemp EO, cannabinoid-containing extracts, and single compounds (i.e., CBD, cannabigerol, cannabinol, and cannabichromene) against a panel of 20 microbial strains demonstrated significant inhibitory activities against Gram-positive bacteria, Helicobacter pylori, and Trichophyton species. In conclusion, this work suggests promising opportunities to use cannabinoid-rich materials from hemp flower processing in functional foods, cosmetics, and pharmaceuticals with antimicrobial properties.
Antifungal Activity of Aniba canelilla (Kunth) Mez Essential Oil and Its Main Compound 1-Nitro-2-Phenylethane against Dermatophytes
Authors: Kreutz Tainá , Duarte Eliane Oliveira Salines , Quatrin Priscilla Maciel , Carneiro Simone Braga , Veiga-Junior Valdir F. , Fuentefria Alexandre Meneghello , Koester Letícia S.
Published: May 2024

The essential oil of Aniba canelilla (Kunth) Mez (EOAC), an Amazon plant composed of a rare nitro compound, has shown scientific evidence of antifungal activity but is still unexplored against dermatophytes. The antifungal susceptibility of EOAC and its main compound, 1-nitro-2-phenylethane (NP), was evaluated against dermatophytes (Trichophyton rubrum, T. mentagrophytes and Microsporum canis), evidencing antifungal activity with an inhibitory concentration lower than 256 μg/mL. The mechanism of action was also evaluated, and it is suggested that EOAC and NP have fungicidal action in the fungal membrane, since the antifungal activity occurs through a modification of the shape of the conidial structures of the fungus, showing the permeability of the intracellular content due to the visually observed plasmolysis and cytosolic extravasation through an osmotic process. These results suggest the essential oil and its main compound are promising plant-derived alternatives for treating ungual dermatophytosis.