bluuebunny/crossref_metadata_2025 huggingface dataset:
Cu/O Frustrated Lewis Pairs on Cu Doped CeO2(111) for Acetylene Hydrogenation: A First-Principles Study
Authors: Zhou Shulan , Wan Qiang , Lin Sen
Published: January 2022

In this work, the H2 dissociation and acetylene hydrogenation on Cu doped CeO2(111) were studied using density functional theory calculations. The results indicated that Cu doping promotes the formation of oxygen vacancy (Ov) which creates Cu/O and Ce/O frustrated Lewis pairs (FLPs). With the help of Cu/O FLP, H2 dissociation can firstly proceed via a heterolytic mechanism to produce Cu-H and O-H by overcoming a barrier of 0.40 eV. The H on Cu can facilely migrate to a nearby oxygen to form another O-H species with a barrier of 0.43 eV. The rate-determining barrier is lower than that for homolytic dissociation of H2 which produces two O-H species. C2H2 hydrogenation can proceed with a rate-determining barrier of 1.00 eV at the presence of Cu-H and O-H species., While C2H2 can be catalyzed by two O-H groups with a rate-determining barrier of 1.06 eV, which is significantly lower than that (2.86 eV) of C2H2 hydrogenated by O-H groups on the bare CeO2(111), showing the high activity of Cu doped CeO2(111) for acetylene hydrogenation. In addition, the rate-determining barrier of C2H4 further hydrogenated by two O-H groups is 1.53 eV, much higher than its desorption energy (0.72 eV), suggesting the high selectivity of Cu doped CeO2(111) for C2H2 partial hydrogenation. This provides new insights to develop effective hydrogenation catalysts based on metal oxide.
Oxysterols in the Immune Response to Bacterial and Viral Infections
Authors: Foo Cheng Xiang , Bartlett Stacey , Ronacher Katharina
Published: January 2022

Oxidized cholesterols, the so-called oxysterols, are widely known to regulate cholesterol homeostasis. However, more recently oxysterols have emerged as important lipid mediators in the response to both bacterial and viral infections. This review summarizes our current knowledge of selected oxysterols and their receptors in the control of intracellular bacterial growth as well as viral entry into the host cell and viral replication. Lastly, we briefly discuss the potential of oxysterols and their receptors as drug targets for infectious and inflammatory diseases.
Transient Exposure of Endothelial Cells to Doxorubicin Leads to Long-Lasting Vascular Endothelial Growth Factor Receptor 2 Downregulation
Authors: Graziani Silvia , Scorrano Luca , Pontarin Giovanna
Published: January 2022

Doxorubicin (Dox) is an effective antineoplastic drug with serious cardiotoxic side effects that persist after drug withdrawal and can lead to heart failure. Dysregulation of vascular endothelium has been linked to the development of Dox-induced cardiotoxicity, but it is unclear whether and how transient exposure to Dox leads to long-term downregulation of Endothelial Vascular Endothelial Growth Factor Receptor type2 (VEGFR2), essential for endothelial cells function. Using an in vitro model devised to study the long-lasting effects of brief endothelial cells exposure to Dox, we show that Dox leads to sustained protein synthesis inhibition and VEGFR2 downregulation. Transient Dox treatment led to the development of long-term senescence associated with a reduction in VEGFR2 levels that persisted days after drug withdrawal. By analyzing VEGFR2 turnover, we ruled out that its downregulation was depended on Dox-induced autophagy. Conversely, Dox induced p53 expression, reduced mTOR-dependent translation, and inhibited global protein synthesis. Our data contribute to a mechanistic basis to the permanent damage caused to endothelial cells by short-term Dox treatment.
Atypical Roles of the Chemokine Receptor ACKR3/CXCR7 in Platelet Pathophysiology
Authors: Chatterjee Madhumita
Published: January 2022

The manifold actions of the pro-inflammatory and regenerative chemokine CXCL12/SDF-1α are executed through the canonical GProteinCoupledReceptor CXCR4, and the non-canonical ACKR3/CXCR7. Platelets express CXCR4, ACKR3/CXCR7, and are a vital source of CXCL12/SDF-1α themselves. In recent years, a regulatory impact of the CXCL12-CXCR4-CXCR7 axis on platelet biogenesis, i.e., megakaryopoiesis, thrombotic and thrombo-inflammatory actions have been revealed through experimental and clinical studies. Platelet surface expression of ACKR3/CXCR7 is significantly enhanced following myocardial infarction (MI) in acute coronary syndrome (ACS) patients, and is also associated with improved functional recovery and prognosis. The therapeutic implications of ACKR3/CXCR7 in myocardial regeneration and improved recovery following an ischemic episode, are well documented. Cardiomyocytes, cardiac-fibroblasts, endothelial lining of the blood vessels perfusing the heart, besides infiltrating platelets and monocytes, all express ACKR3/CXCR7. This review recapitulates ligand induced differential trafficking of platelet CXCR4-ACKR3/CXCR7 affecting their surface availability, and in regulating thrombo-inflammatory platelet functions and survival through CXCR4 or ACKR3/CXCR7. It emphasizes the pro-thrombotic influence of CXCL12/SDF-1α exerted through CXCR4, as opposed to the anti-thrombotic impact of ACKR3/CXCR7. Offering an innovative translational perspective, this review also discusses the advantages and challenges of utilizing ACKR3/CXCR7 as a potential anti-thrombotic strategy in platelet-associated cardiovascular disorders, particularly in coronary artery disease (CAD) patients post-MI.
One-Carbon Metabolism: Pulling the Strings behind Aging and Neurodegeneration
Authors: Lionaki Eirini , Ploumi Christina , Tavernarakis Nektarios
Published: January 2022

One-carbon metabolism (OCM) is a network of biochemical reactions delivering one-carbon units to various biosynthetic pathways. The folate cycle and methionine cycle are the two key modules of this network that regulate purine and thymidine synthesis, amino acid homeostasis, and epigenetic mechanisms. Intersection with the transsulfuration pathway supports glutathione production and regulation of the cellular redox state. Dietary intake of micronutrients, such as folates and amino acids, directly contributes to OCM, thereby adapting the cellular metabolic state to environmental inputs. The contribution of OCM to cellular proliferation during development and in adult proliferative tissues is well established. Nevertheless, accumulating evidence reveals the pivotal role of OCM in cellular homeostasis of non-proliferative tissues and in coordination of signaling cascades that regulate energy homeostasis and longevity. In this review, we summarize the current knowledge on OCM and related pathways and discuss how this metabolic network may impact longevity and neurodegeneration across species.
Komagataella phaffii Cue5 Piggybacks on Lipid Droplets for Its Vacuolar Degradation during Stationary Phase Lipophagy
Authors: Kumar Ravinder , Shroff Ankit , Nazarko Taras Y.
Published: January 2022

Recently, we developed Komagataella phaffii (formerly Pichia pastoris) as a model for lipophagy, the selective autophagy of lipid droplets (LDs). We found that lipophagy pathways induced by acute nitrogen (N) starvation and in stationary (S) phase have different molecular mechanisms. Moreover, both types of lipophagy are independent of Atg11, the scaffold protein that interacts with most autophagic receptors and, therefore, is essential for most types of selective autophagy in yeast. Since yeast aggrephagy, the selective autophagy of ubiquitinated protein aggregates, is also independent of Atg11 and utilizes the ubiquitin-binding receptor, Cue5, we studied the relationship of K. phaffii Cue5 with differentially induced LDs and lipophagy. While there was no relationship of Cue5 with LDs and lipophagy under N-starvation conditions, Cue5 accumulated on LDs in S-phase and degraded together with LDs via S-phase lipophagy. The accumulation of Cue5 on LDs and its degradation by S-phase lipophagy strongly depended on the ubiquitin-binding CUE domain and Prl1, the positive regulator of lipophagy 1. However, unlike Prl1, which is required for S-phase lipophagy, Cue5 was dispensable for it suggesting that Cue5 is rather a new substrate of this pathway. We propose that a similar mechanism (Prl1-dependent accumulation on LDs) might be employed by Prl1 to recruit another ubiquitin-binding protein that is essential for S-phase lipophagy.
TFEB Regulates ATP7B Expression to Promote Platinum Chemoresistance in Human Ovarian Cancer Cells
Authors: Petruzzelli Raffaella , Mariniello Marta , De Cegli Rossella , Catalano Federico , Guida Floriana , Di Schiavi Elia , Polishchuk Roman S.
Published: January 2022

ATP7B is a hepato-specific Golgi-located ATPase, which plays a key role in the regulation of copper (Cu) homeostasis and signaling. In response to elevated Cu levels, ATP7B traffics from the Golgi to endo-lysosomal structures, where it sequesters excess copper and further promotes its excretion to the bile at the apical surface of hepatocytes. In addition to liver, high ATP7B expression has been reported in tumors with elevated resistance to platinum (Pt)-based chemotherapy. Chemoresistance to Pt drugs represents the current major obstacle for the treatment of large cohorts of cancer patients. Although the mechanisms underlying Pt-tolerance are still ambiguous, accumulating evidence suggests that lysosomal sequestration of Pt drugs by ion transporters (including ATP7B) might significantly contribute to drug resistance development. In this context, signaling mechanisms regulating the expression of transporters such as ATP7B are of great importance. Considering this notion, we investigated whether ATP7B expression in Pt-resistant cells might be driven by transcription factor EB (TFEB), a master regulator of lysosomal gene transcription. Using resistant ovarian cancer IGROV-CP20 cells, we found that TFEB directly binds to the predicted coordinated lysosomal expression and regulation (CLEAR) sites in the proximal promoter and first intron region of ATP7B upon Pt exposure. This binding accelerates transcription of luciferase reporters containing ATP7B CLEAR regions, while suppression of TFEB inhibits ATP7B expression and stimulates cisplatin toxicity in resistant cells. Thus, these data have uncovered a Pt-dependent transcriptional mechanism that contributes to cancer chemoresistance and might be further explored for therapeutic purposes.
Energy Metabolism and Hyperactivation of Spermatozoa from Three Mouse Species under Capacitating Conditions
Authors: Sansegundo Ester , Tourmente Maximiliano , Roldan Eduardo R. S.
Published: January 2022

Mammalian sperm differ widely in sperm morphology, and several explanations have been presented to account for this diversity. Less is known about variation in sperm physiology and cellular processes that can give sperm cells an advantage when competing to fertilize oocytes. Capacitation of spermatozoa, a process essential for mammalian fertilization, correlates with changes in motility that result in a characteristic swimming pattern known as hyperactivation. Previous studies revealed that sperm motility and velocity depend on the amount of ATP available and, therefore, changes in sperm movement occurring during capacitation and hyperactivation may involve changes in sperm bioenergetics. Here, we examine differences in ATP levels of sperm from three mouse species (genus Mus), differing in sperm competition levels, incubated under non-capacitating and capacitating conditions, to analyse relationships between energetics, capacitation, and swimming patterns. We found that, in general terms, the amount of sperm ATP decreased more rapidly under capacitating conditions. This descent was related to the development of a hyperactivated pattern of movement in two species (M. musculus and M. spicilegus) but not in the other (M. spretus), suggesting that, in the latter, temporal dynamics and energetic demands of capacitation and hyperactivation may be decoupled or that the hyperactivation pattern differs. The decrease in ATP levels during capacitation was steeper in species with higher levels of sperm competition than in those with lower levels. Our results suggest that, during capacitation, sperm consume more ATP than under non-capacitating conditions. This higher ATP consumption may be linked to higher velocity and lateral head displacement, which are associated with hyperactivated motility.
White Phosphate Coatings Obtained on Steel from Modified Cold Phosphating Solutions
Authors: Rumyantsev Evgeniy , Rumyantseva Varvara , Konovalova Viktoriya
Published: January 2022

The article presents a method for obtaining white phosphate coatings on steel by cold method. The deposition of protective phosphate coatings was carried out from solutions based on the preparation “Majef”, consisting of manganese and iron phosphates. To obtain phosphate films of white color, it is proposed to introduce zinc and calcium nitrates into phosphating solutions at the rate of 25–30 g/L. The surface of phosphate coatings was studied using the SolverP47-PRO atomic force microscope images, and the average grain size was determined. The structural and phase composition of phosphate coatings was been studied using X-ray diffraction analysis. The protective properties of phosphate coatings were estimated by corrosion rate indicators calculated from corrosion diagrams. Fine-crystalline uniform coatings were obtained from modified phosphating solutions at room temperature on steel. The white color of phosphate coatings is due to the increased content of phosphophyllite, hopeite, and parascholzite in their structural and phase composition. By applying protective phosphate coatings of white color on a steel product, corrosion can be slowed down by 4–4.5 times. However, white phosphate coatings are inferior in protective properties to unpainted coatings. The index of change in the mass of samples with white phosphate coatings because of corrosion is 0.371–0.41 g/(m2·h), and with unpainted coatings is 0.128 g/(m2·h).
Coatings Functionalization via Laser versus Other Deposition Techniques for Medical Applications: A Comparative Review
Authors: Badiceanu Maria , Anghel Sinziana , Mihailescu Natalia , Visan Anita Ioana , Mihailescu Cristian N. , Mihailescu Ion N.
Published: January 2022

The development of new biological devices in response to market demands requires continuous efforts for the improvement of products’ functionalization based upon expansion of the materials used and their fabrication techniques. One viable solution consists of a functionalization substrate covered by layers via an appropriate deposition technique. Laser techniques ensure an enhanced coating’s adherence to the substrate and improved biological characteristics, not compromising the mechanical properties of the functionalized medical device. This is a review of the main laser techniques involved. We mainly refer to pulse laser deposition, matrix-assisted, and laser simple and double writing versus some other well-known deposition methods as magnetron sputtering, 3D bioprinting, inkjet printing, extrusion, solenoid, fuse-deposition modeling, plasma spray (PS), and dip coating. All these techniques can be extended to functionalize surface fabrication to change local morphology, chemistry, and crystal structure, which affect the biomaterial behavior following the chosen application. Surface functionalization laser techniques are strictly controlled within a confined area to deliver a large amount of energy concisely. The laser deposit performances are presented compared to reported data obtained by other techniques.
The Potential of the Superhydrophobic State to Protect Magnesium Alloy against Corrosion
Authors: Emelyanenko Kirill A. , Chulkova Elizaveta V. , Semiletov Alexey M. , Domantovsky Alexander G. , Palacheva Valeria V. , Emelyanenko Alexandre M. , Boinovich Ludmila B.
Published: January 2022

We describe the technologically simple route for the fabrication of the superhydrophobic coatings on top of wrought magnesium alloy MA8 based on nanosecond laser processing followed by the chemical vapor deposition of fluorosilane. The chemical and phase composition, surface morphologies, and variation of the coating wettability during prolonged contact with 0.5 NaCl solution or with salt aerosol were characterized using X-ray diffraction, FT-IR spectroscopy, scanning electron microscopy measurements, and the wettability analysis. The as-prepared coatings demonstrate corrosion current of more than eight orders of magnitude lower, while after 30 days of sample immersion into corrosive solution, the current was four orders of magnitude lower than that obtained for a polished sample which was for only 2 h in contact with electrolyte. The mechanisms of the protective activity of fabricated coatings were discussed.
Characterization of Thickness Loss in a Storage Tank Plate with Piezoelectric Wafer Active Sensors
Authors: Liu Wencai , Fan Jianchun , Yang Jin
Published: January 2022

In terms of the structural health inspection of storage tanks by ultrasonic guided wave technology, many scholars are currently focusing on the tanks’ floor and walls, while little research has been conducted on storage tank roofs. However, the roof of a storage tank is prone to corrosion because of its complex structure and unique working environment. For this purpose, this paper proposes a reflection/transmission signal amplitude ratio (RTAR) coefficient method for corrosion depth assessment. We studied the relationship between the RTAR coefficient, the corrosion depth, and the guided wave frequency to establish a depth assessment model. More importantly, unlike the traditional reflection coefficient method, the characteristics of guided wave signals, including the propagation and attenuation, are introduced in this model for accurate assessment. To eliminate the interference of residual vibration and improve the detection accuracy of defects, we built a corrosion detection system by using piezoelectric sensors and carried out field tests to verify the performance of the proposed method. We demonstrate that corrosion defects with a minimum depth of 0.2 mm can be quantitatively evaluated.
Neoadjuvant and Adjuvant Systemic Therapy for Newly Diagnosed Stage II–IV Epithelial Ovary, Fallopian Tube, or Primary Peritoneal Carcinoma: A Practice Guideline
Authors: Hirte Hal , Poon Raymond , Yao Xiaomei , May Taymaa , Ethier Josee-Lyne , Petz Lauri , Speakman Jane , Elit Laurie
Published: January 2022

Background: This study aims to provide guidance for the use of neoadjuvant and adjuvant systemic therapy in women with newly diagnosed stage II–IV epithelial ovary, fallopian tube, or primary peritoneal carcinoma. Methods: EMBASE, MEDLINE, and Cochrane Library were investigated for relevant systematic reviews and phase III trials. Articles focusing on consolidation and maintenance therapies were excluded. Results: For women with potentially resectable disease, primary cytoreductive surgery, followed by six to eight cycles of intravenous three-weekly paclitaxel and carboplatin is recommended. For those with a high-risk profile for primary cytoreductive surgery, neoadjuvant chemotherapy can be an option. Adjuvant chemotherapy with six cycles of dose-dense weekly paclitaxel plus three-weekly carboplatin can be considered for women of Japanese descent. In women with stage III or IV disease, the incorporation of bevacizumab concurrent with paclitaxel and carboplatin is not recommended for use as adjuvant therapy unless bevacizumab is continued as maintenance therapy. Intravenous paclitaxel plus intraperitoneal cisplatin and paclitaxel can be considered for stage III optimally debulked women who did not receive neoadjuvant chemotherapy. However, intraperitoneal administration of chemotherapy with bevacizumab should not be considered as an option for stage II–IV optimally debulked women. Discussion: The recommendations represent a current standard of care that is feasible to implement and valued by both clinicians and patients.
Multi-Gene Phylogeny and Taxonomy of Hypoxylon (Hypoxylaceae, Ascomycota) from China
Authors: Ma Haixia , Song Zikun , Pan Xiaoyan , Li Yu , Yang Zhanen , Qu Zhi
Published: January 2022

The Hypoxylon species play an important ecological role in tropical rainforest as wood-decomposers, and some might have benefical effects on their hosts as endophytes. The present work concerns a survey of the genus Hypoxylon from Hainan Tropical Rainforest National Park of China. Four new species: H. wuzhishanense, H. hainanense, H.chrysalidosporum, and H.cyclobalanopsidis, were discovered based on a combination of morphological characteristics and molecular data. Hypoxylon wuzhishanense is characterized by Rust pulvinate stromata, amyloid apical apparatus and brown ascospores, with most of the perispore being indehiscent in 10% KOH. Hypoxylon hainanense has effused–pulvinate and Violet stromata, amyloid apical apparatus, light-brown to brown ascospores with straight germ slit and dehiscent perispore. Hypoxylonchrysalidosporum is distinguished by glomerate to pulvinate stromata, highly reduced or absent inamyloid apical apparatus, and light-brown to brown ascospores with very conspicuous coil-like ornamentation. Hypoxyloncyclobalanopsidis has Livid Purple pulvinate stromata, highly reduced amyloid apical apparatus, faint bluing, brown ascospores and dehiscent perispore, and it grows on dead branches of Cyclobalanopsis. Detailed descriptions, illustrations, and contrasts with morphologically similar species are provided. Phylogenetic analyses inferred from ITS, RPB2, LSU, and β-tubulin sequences confirmed that the four new species are distinct within the genus Hypoxylon.
Basaloid Follicular Hamartoma of the Eyelid: A Case Report and Literature Review about an Unusual Lesion in the Ocular Region
Authors: Lamas Nuno Jorge , Rodrigues Ana Patrícia , Araújo Maria , Vizcaíno José Ramón , Coelho André
Published: January 2022

Basaloid follicular hamartoma (BFH) is a normally benign, uncommon, malformative lesion involving the hair follicles, which usually poses challenges in the differential diagnosis with other benign and malignant tumours, especially basal cell carcinoma, due to significant clinical and morphological overlap. Here, we report the case of a 53-year-old male who presented with a mass in the upper left eyelid evolving for one year. The patient had a previous history of total colectomy and an abdominal desmoid tumour within the context of Familial Adenomatous Polyposis (FAP), with a documented germline mutation in the Adenomatous Polyposis Coli (APC) gene. The eyelid lesion was biopsied and the histological analysis of the three small tissue fragments received revealed fragments with cutaneous–conjunctival lining displaying a subepithelial proliferation of basaloid nests with peripheral palisading, compatible with primitive hair follicles. There were images of anastomosis between different basaloid nests, which had their connection to the epithelial lining preserved. The stroma had high cellularity and sometimes primitive mesenchymal papillae were evident. Pleomorphism was absent, mitotic figures were barely identified, and no necrosis was seen. The basaloid nests did not have epithelial–stromal retraction nor mucin deposits. A diagnosis of BFH was proposed, which was later confirmed after surgical excision of the whole eyelid lesion. No evidence of carcinoma was present. This case illustrates the main features of the rare benign eyelid BFH. The standard medical or surgical approach of these lesions remains to be firmly established. Nearly nine months after surgical excision our patient remains well without signs of disease recurrence.
Evaluation of Shoulder Microcirculation Abnormality Using Laser Doppler Flowmetry
Authors: Chia Taipau , Bau Jian-Guo , Hung Guo-Dung , Tsai Sz-Huan , Hu Che-Ming
Published: January 2022

Severe neck-shoulder pain induces functional limitations in both life and work. The purpose of this study was to determine the characteristics of shoulder microcirculation abnormality in workers. This study recruited 32 workers and patients, both n = 16. Questionnaires were administered, and Laser Doppler Flowmetry (LDF) was used to measure microcirculatory blood flow (MBF) at the myofascial trigger points (MTrPs) on the shoulders. The absolute-deviationMMBF represented the mean MBF (MMBF) variability among subjects. The differences in the life characteristics, shoulder pain level, and microcirculatory characteristics at MTrPs between the two groups were compared. It was found that shoulder pain level was significantly higher in the patient than in the control group (p < 0.001). Deviation of the MMBF value beyond the postulated “normal range” of 60–80 was significantly higher in the patient than in the control group (p < 0.001). The MMBF deviation was significantly correlated with shoulder pain level, pain duration, and the symptom effect (p < 0.01, n = 32). A normal range for the MMBF of 60–80 on the shoulder near MTrPs is hypothesized for the first time based on this study. Noninvasive LDF can be used to assess abnormality in the MBF on shoulder MTrPs at an early stage.
Significance of Pelvic Fluid Observed during Ovarian Cancer Screening with Transvaginal Sonogram
Authors: Gorski Justin W. , Dietrich Charles S. , Davis Caeli , Erol Lindsay , Dietrich Hayley , Per Nicholas J. , Ferrell Emily Lenk , McDowell Anthony B. , Riggs McKayla J. , Hutchcraft Megan L. , Baldwin-Branch Lauren A. , Miller Rachel W. , DeSimone Christopher P. , Gallion Holly H. , Ueland Frederick R. , van Nagell John R. , Pavlik Edward J.
Published: January 2022

The primary objective was to examine the role of pelvic fluid observed during transvaginal ultrasonography (TVS) in identifying ovarian malignancy. A single-institution, observational study was conducted within the University of Kentucky Ovarian Cancer Screening trial from January 1987 to September 2019. We analyzed true-positive (TP), false-positive (FP), true-negative (TN), and false-negative (FN) groups for the presence of pelvic fluid during screening encounters. Measured outcomes were the presence and duration of fluid over successive screening encounters. Of the 48,925 women surveyed, 2001 (4.1%) had pelvic fluid present during a TVS exam. The odds ratio (OR) of detecting fluid in the comparison group (TN screen; OR = 1) significantly differed from that of the FP cases (benign pathology; OR: 13.4; 95% confidence interval (CI): 9.1–19.8), the TP cases with a low malignant potential (LMP; OR: 28; 95% CI: 26.5–29.5), TP ovarian cancer cases (OR: 50.4; 95% CI: 27.2–93.2), and FN ovarian cancer cases (OR: 59.3; 95% CI: 19.7–178.1). The mean duration that pelvic fluid was present for women with TN screens was 2.2 ± 0.05 encounters, lasting 38.7 ± 1.3 months. In an asymptomatic screening population, free fluid identified in TVS exams was more associated with ovarian malignancy than in the control group or benign ovarian tumors. While pelvic free fluid may not solely discriminate malignancy from non-malignancy, it appears to be clinically relevant and warrants thoughtful consideration.
Relationships of Pelvic Vein Diameter and Reflux with Clinical Manifestations of Pelvic Venous Disorder
Authors: Gavrilov Sergey , Karalkin Anatoly , Mishakina Nadezhda , Efremova Oksana , Grishenkova Anastasia
Published: January 2022

The causes of chronic pelvic pain (CPP) in patients with pelvic venous disorder (PeVD) are not completely understood. Various authors consider dilation of pelvic veins (PeVs) and pelvic venous reflux (PVR) as the main mechanisms underlying symptomatic forms of PeVD. The aim of this study was to assess relationships of pelvic vein dilation and PVR with clinical manifestations of PeVD. This non-randomized comparative cohort study included 80 female patients with PeVD who were allocated into two groups with symptomatic (n = 42) and asymptomatic (n = 38) forms of the disease. All patients underwent duplex scanning and single-photon emission computed tomography (SPECT) of PeVs with in vivo labeled red blood cells (RBCs). The PeV diameters, the presence, duration and pattern of PVR in the pelvic veins, as well as the coefficient of pelvic venous congestion (CPVC) were assessed. Two groups did not differ significantly in pelvic vein diameters (gonadal veins (GVs): 7.7 ± 1.3 vs. 8.5 ± 0.5 mm; parametrial veins (PVs): 9.8 ± 0.9 vs. 9.5 ± 0.9 mm; and uterine veins (UVs): 5.6 ± 0.2 vs. 5.5 ± 0.6 mm). Despite this, CPVC was significantly higher in symptomatic versus asymptomatic patients (1.9 ± 0.4 vs. 0.7 ± 0.2, respectively; p = 0.008). Symptomatic patients had type II or III PVR, while asymptomatic patients had type I PVR. The reflux duration was found to be significantly greater in symptomatic versus asymptomatic patients (median and interquartile range: 4.0 [3.0; 5.0] vs. 1.0 [0; 2.0] s for GVs, p = 0.008; 4.0 [3.0; 5.0] vs. 1.1 [1.0; 2.0] s for PVs, p = 0.007; and 2.0 [2.0; 3.0] vs. 1.0 [1.0; 2.0] s for UVs, p = 0.04). Linear correlation analysis revealed a strong positive relationship (Pearson’s r = 0.78; p = 0.007) of CPP with the PVR duration but not with vein diameter. The grade of PeV dilation may not be a determining factor in CPP development in patients with PeVD. The presence and duration of reflux in the pelvic veins were found to be predictors of the development of symptomatic PeVD.
Corneal Endothelial Changes Following Early Capsulotomy Using Neodymium:Yttrium–Aluminum–Garnet Laser
Authors: Chen Hung-Chi , Lee Chia-Yi , Liu Chun-Fu , Hsueh Yi-Jen , Meir Yaa-Jyuhn James , Cheng Chao-Min , Wu Wei-Chi
Published: January 2022

We aimed to survey whether the timing of neodymium:yttrium–aluminum–garnet (Nd:YAG) laser capsulotomy would alter the corneal endothelial morphology and density. A retrospective cohort study was conducted, and 48 patients with unilateral posterior capsular opacity (PCO) and Nd:YAG laser capsulotomy performance were enrolled. The participants were divided into the early Nd:YAG group (timing ≤ 12 months, n = 20) and late Nd:YAG group (timing > 12 months, n= 28) depending on elapsed months between phacoemulsification and Nd:YAG laser capsulotomy. Endothelial cell density (ECD), coefficient of variant (CV), hexagonality (HEX), and central corneal thickness (CCT) between the two groups were collected. A generalized estimate equation was conducted to evaluate the corneal endothelial parameters between the two groups with an adjusted odds ratio (aOR) and 95% confidence interval (CI). The CDVA was improved after treatment in both groups (both p < 0.001). Chronically, ECD in the early group was significantly decreased one week after treatment (2221.50 ± 327.73/mm2 vs. 2441.55 ± 321.80/mm2, p < 0.001), which recovered to 2369.95 ± 76.37/mm2 four weeks after the treatment but was still lower than the preoperative status (p < 0.001). In addition, the HEX percentage showed a significant reduction at four weeks after treatment (p = 0.028). The ECD in the early group was significantly lower than that in the late group (aOR: 0.167, 95% CI: 0.079–0.356, p = 0.003) in both week 1 (p < 0.001) and week 4 (p = 0.004) after laser treatment. In conclusion, the early application of Nd:YAG laser capsulotomy within one year after cataract surgery may be the reason for postoperative ECD decrement without known etiology.
Coronary Artery Disease and Gallbladder Inflammatory Pseudopolyps
Authors: Fosio Margherita , Cherobin Giulia , Stramare Roberto , Fassan Matteo , Giraudo Chiara
Published: January 2022

Axial MR image demonstrating multiple small gallbladder polypoid lesions characterized by contrast enhancement in a 78-year-old male hospitalized for acute chest pain due to coronary artery disease who showed fever and emesis during hospitalization and had signs of acute acalculous cholecystitis at computed tomography. Given the overall clinical conditions and the MR features, the inflammatory origin of the polyps was considered. The patient underwent cholecystectomy and the histological diagnosis of gallbladder inflammatory pseudopolyps was confirmed. This rare entity represents 5–10% of all gallbladder polyps, and their differentiation from benign and malignant tumors might be challenging especially in acalculous patients, thus surgery is often performed.
The Diagnostic and Prognostic Utility of Contemporary Cardiac Magnetic Resonance in Suspected Acute Myocarditis
Authors: Lagan Jakub , Fortune Christien , Hutchings David , Bradley Joshua , Naish Josephine H. , Timoney Richard , Prescott Daniel , Bain Hamish D. C. , Bangi Tasneem , McIntosh Jerome , Egdell Robin , Irwin R. Bruce , Clark David , Schelbert Erik B. , Nucifora Gaetano , Schmitt Matthias , Miller Christopher A.
Published: January 2022

Cardiovascular magnetic resonance (CMR) is used to investigate suspected acute myocarditis, however most supporting data is retrospective and few studies have included parametric mapping. We aimed to investigate the utility of contemporary multiparametric CMR in a large prospective cohort of patients with suspected acute myocarditis, the impact of real-world variations in practice, the relationship between clinical characteristics and CMR findings and factors predicting outcome. 540 consecutive patients we recruited. The 113 patients diagnosed with myocarditis on CMR performed within 40 days of presentation were followed-up for 674 (504–915) days. 39 patients underwent follow-up CMR at 189 (166–209) days. CMR provided a positive diagnosis in 72% of patients, including myocarditis (40%) and myocardial infarction (11%). In multivariable analysis, male sex and shorter presentation-to-scan interval were associated with a diagnosis of myocarditis. Presentation with heart failure (HF) was associated with lower left ventricular ejection fraction (LVEF), higher LGE burden and higher extracellular volume fraction. Lower baseline LVEF predicted follow-up LV dysfunction. Multiparametric CMR has a high diagnostic yield in suspected acute myocarditis. CMR should be performed early and include parametric mapping. Patients presenting with HF and reduced LVEF require closer follow-up while those with normal CMR may not require it.
Second Trimester Fetal Loss Due to Citrobacter koseri Infection: A Rare Cause of Preterm Premature Rupture of Membranes (PPROM)
Authors: Bonasoni Maria Paola , Comitini Giuseppina , Pati Mariangela , Russello Giuseppe , Vizzini Loredana , Bardaro Marcellino , Pini Pietro , Marrollo Roberta , Palicelli Andrea , Dalla Dea Giulia , Carretto Edoardo
Published: January 2022

Citrobacter koseri is a facultative anaerobic, motile, non-spore-forming Gram-negative bacillus, which belongs to the family of Enterobacteriaceae. Severe infections due to Citrobacter spp. have been reported in the urinary tract, respiratory airways, intra-abdominal organs, skin and soft tissue, eye, bone, bloodstream, and central nervous system. In newborns, C. koseri is a well-known cause of meningitis, cerebral abscesses, brain adhesions, encephalitis, and pneumocephalus. Infection can be acquired through vertical maternal transmission or horizontal hospital settings; however, in many cases, the source is unknown. Preterm premature rupture of membranes (PPROM), caused by C. koseri, has rarely been described. Herein, we describe a case of PPROM at 16 weeks and 3 days of gestation, leading to anhydramnios. The parents opted for legal termination of the pregnancy, as the prognosis was very poor. C. koseri was isolated postmortem from a placental subamniotic swab and parenchymal sample, as well as fetal blood and lung. To the best of our knowledge, this is the first case of early second-trimester PPROM in which C. koseri infection was demonstrated.
Thrown into Deep Water: Feedback on Student Satisfaction—A Case Study in Hungarian and Romanian Universities
Authors: Harangi-Rákos Mónika , Ștefănescu Daniela , Zsidó Kinga-Emese , Fenyves Veronika
Published: January 2022

As a result of the COVID-19 health epidemic, online life has exploded into our daily lives, forcing most of us to move previously seemingly irreplaceable “face-to-face” activities into “non-face-to-face” meetings and activities in many sectors. One of the biggest challenges has been in the field of education: This sector, compared to other sectors, was less digitized. Under these circumstances, the entire education process was transferred to online space overnight, which was/is a major challenge for everyone. Thus, a questionnaire survey was conducted among students from two universities in Hungary and Romania, the results of which are included in the present article. The aim of the research was to measure students’ satisfaction and to examine the benefits of online education, for example, in terms of introducing hybrid education over the long term. Descriptive statistics as well as the Wilcoxon rank-sum test were used to analyze the database. The results showed that, from a practical point of view, there was no significant difference between the Hungarian and Romanian respondents who had a fundamentally positive view of digital education. In this respect, positive feedback can be seen as encouraging, especially for those individuals and social strata who may find online education much more attractive than traditional physical teaching. Online education could be an attractive, accessible, sustainable form of further education in the long run.
Informal Education Pedagogy Transcendence from the ‘Academy’ to Society in the Current and Post COVID Environment
Authors: Jones Ian David , Brady Geraldine
Published: January 2022

The purpose of this paper is to consider the following two notions; (1) that the use of ‘informal education pedagogies’ within teaching and learning in the ‘academy’ can both support the learning process within the ‘classroom’ but also transcend to society via students; and (2) that synergies exist between informal education and social pedagogical concepts. The discussions are situated from the perspective of an experienced practitioner and academic who is currently teaching youth related degree courses within a Higher Education Institution. This experiential learning has informed knowledge acquisition, understanding and skills application from professional practice to the teaching environment. An experiential learning perspective will be the primary method adopted; the value of this paper lies in its potential to re-affirm that degree courses which embed a ‘practice the practice’ approach in their teaching methodology support the embedding of core values of the said discipline. The paper argues that the ethically value-based principles and practice of informal education pedagogy, and social pedagogy, are relevant for the current and post COVID-19 pandemic environment.
Computational Thinking and Educational Technology: A Scoping Review of the Literature
Authors: Acevedo-Borrega Jesús , Valverde-Berrocoso Jesús , Garrido-Arroyo María del Carmen
Published: January 2022

Interest in computational thinking (CT) in the scientific community has increased significantly in the last 4 years, as evidenced by the numerous systematic reviews carried out. However, there is a lack of reviews that update the emerging conceptualization of CT and which also examine the roles of the school curriculum and teachers in the face of CT. A systematic literature review (SLR) consists of a collection of research conducted according to previous criteria with the aim of answering research questions with validity and quality. For this reason, the PRISMA-ScR statement was followed. Articles published in scientific journals, from Scopus and WoS, between January 2018 and August 2021 were included, in the English or Spanish language. The initial search resulted in 492 articles, to which the inclusion-exclusion criteria were applied. The final sample of texts for the present systematic review was n = 145. The texts were analyzed from three perspectives: conceptual, documentary and pedagogical. Thus, a renewal of previous literature reviews was carried out, updating the situation with research from recent years and new data, obtained to contribute to the collective intelligence on methodological strategies (80% of the sample was divided into “plugged” and “unplugged”); educational (more than 50% studied CT evaluation); and resources, including a collection of more than 119 educational resources.
Research on Co-Estimation Algorithm of SOC and SOH for Lithium-Ion Batteries in Electric Vehicles
Authors: Du Chang-Qing , Shao Jian-Bo , Wu Dong-Mei , Ren Zhong , Wu Zhong-Yi , Ren Wei-Qun
Published: January 2022

The accurate estimation of the state of charge (SOC) and state of health (SOH) is of great significance to energy management and safety in electric vehicles. To achieve a good trade-off between real-time capability and estimation accuracy, a collaborative estimation algorithm for SOC and SOH is presented based on the Thevenin equivalent circuit model, which combines the recursive least squares method with a forgetting factor and the extended Kalman filter. First, the parameter identification accuracy is studied under a dynamic stress test (DST) and the federal urban driving schedule (FUDS) test at different ambient temperatures (0 °C, 25 °C, and 45 °C). Secondly, the FUDS test is used to verify the SOC estimation accuracy. Thirdly, two batteries with different aging degrees are used to validate the proposed SOH estimation algorithm. Subsequently, the accuracy of the SOC estimation algorithm is studied, considering the influence of updating the SOH. The proposed SOC estimation algorithm can achieve good performance at different ambient temperatures (0 °C, 25 °C, and 45 °C), with a maximum error of less than 2.3%. The maximum error for the SOH is less than 4.3% for two aged batteries at 25 °C, and it can be reduced to 1.4% after optimization. Furthermore, calibrating the capacity as the SOH changes can effectively improve the SOC estimation accuracy over the whole battery life.
Small-Signal Analysis of All-Si Microring Resonator Photodiode
Authors: Peng Yiwei , Sorin Wayne V. , Cheung Stanley , Yuan Yuan , Huang Zhihong , Fiorentino Marco , Beausoleil Raymond G.
Published: January 2022

All-silicon microring resonator photodiodes are attractive for silicon photonics integrated circuits due to their compactness, wavelength division multiplexing ability, and the absence of germanium growth. To analyze and evaluate the performance of the microring photodiode, we derived closed-form expression of the response transfer function with both electrical and optical behavior included, using a small-signal analysis. The thermo-optic nonlinearity resulting from optical loss and ohmic heating was simulated and considered in the model. The predicted response achieved close agreement with the experiment results, which provides an intuitive understanding of device performance. We analytically investigated the responsivity–bandwidth product and demonstrated that the performance is superior when the detuning frequency is zero.
Counteracting Selfish Nodes Using Reputation Based System in Mobile Ad Hoc Networks
Authors: Fayaz Muhammad , Mehmood Gulzar , Khan Ajab , Abbas Sohail , Fayaz Muhammad , Gwak Jeonghwan
Published: January 2022

A mobile ad hoc network (MANET) is a group of nodes constituting a network of mobile nodes without predefined and pre-established architecture where mobile nodes can communicate without any dedicated access points or base stations. In MANETs, a node may act as a host as well as a router. Nodes in the network can send and receive packets through intermediate nodes. However, the existence of malicious and selfish nodes in MANETs severely degrades network performance. The identification of such nodes in the network and their isolation from the network is a challenging problem. Therefore, in this paper, a simple reputation-based scheme is proposed which uses the consumption and contribution information for selfish node detection and cooperation enforcement. Nodes failing to cooperate are detached from the network to save resources of other nodes with good reputation. The simulation results show that our proposed scheme outperforms the benchmark scheme in terms of NRL (normalized routing load), PDF (packet delivery fraction), and packet drop in the presence of malicious and selfish attacks. Furthermore, our scheme identifies the selfish nodes quickly and accurately as compared to the benchmark scheme.
Software in-the-Loop Simulation of an Advanced SVM Technique for 2ϕ-Inverter Control Fed a TPIM as Wind Turbine Emulator
Authors: Moussa Intissar , Khedher Adel
Published: January 2022

An appropriate modulation scheme selection ensures inverter performance. Thus, space vector modulation (SVM) is more efficient and has its own distinct advantages compared to other pulse width modulation (PWM) techniques. This work deals with the development of an advanced space vector pulse width modulation (SVM) technique for two-phase inverter control using an XSG library to ensure rapid prototyping of the controller FPGA implementation. The proposed architecture is applied digitally and in real time to drive a two-phase induction motor (TPIM) for small-scale wind turbine emulation (WTE) profiles in laboratories with minimum current ripple and torque oscillation. Four space voltage vectors generated for the used SVM technique do not contain a zero vector. Hence, for an adequate adjustment of these four vectors, a reference voltage vector located in the square locus is determined. Considering the asymmetry between the main and auxiliary windings, the TPIM behavior, which is fed through the advanced SVM controlled-two-phase inverter (2ϕ-inverter), is studied, allowing us to control the speed and the torque under different conditions for wind turbine emulation. Several quantities, such as electromagnetic torque, rotor fluxes, stator currents and speed, are analyzed. To validate the obtained results using both Simulink and XSG interfaces, the static and dynamic characteristics of the WTE are satisfactorily reproduced. The collected speed and torque errors between the reference and actual waveforms show low rates, proving emulator controller effectiveness.
Secure Authentication Scheme Using Diffie–Hellman Key Agreement for Smart IoT Irrigation Systems
Authors: Nashwan Shadi
Published: January 2022

Smart irrigation is considered one of the most significant agriculture management systems worldwide, considering the current context of water scarcity. There is a clear consensus that such smart systems will play an essential role in achieving the economic growth of other vital sectors. In general, the consequences of global warming and the unavailability of clean water sources for the agricultural sector are clear indications that the demand for these systems will increase in the near future, especially considering the recent expansions in the use of the Internet of Things (IoT) and Wireless Sensor Network (WSN) technologies, which have been employed in the development of such systems. An obvious result is that security challenges will be one of the main obstacles to attaining the widespread adoption of such systems. Therefore, this paper proposes a secure authentication scheme using Diffie–Hellman key agreement for smart IoT irrigation systems using WSNs. This scheme is based on Diffie–Hellman and one-way hash cryptographic functions in order to support the basic security services with a high data rate and ability to resist well-known attacks. The Burrows–Abadi–Needham (BAN) logic model is used to verify the proposed scheme formally. Based on various possible attack scenarios, a resistance analysis of the proposed scheme is discussed. Further analyses are performed in terms of the storage size, intercommunication, and running time costs. Therefore, the proposed scheme not only can be considered a secure authentication scheme but is also practical for smart IoT irrigation systems due to its reasonable efficiency factors.