bluuebunny/crossref_metadata_2025 huggingface dataset:
Study of some effective factors on the production of garamicidin from locally isolated Bacillus brevis
Authors: Journal Baghdad Science
Published: March 2019

The optimum cultural conditions for garamicidin production by local isolate B.brevis were studied.Best result was obtained when the isolate B.brevis was grown on media composed of 1%glucose as carbon source,1% ammonium chloride as a nitrogen source ,0.5% Dipotassium hydrogen orthophosphate as a phosphate source and after 48 hours of incubation at 30C .Garamicidin has been extracted and purified through acid precipition and then extracted by organic solvent (ether& acetone ).Using HPLC the garamicidin antibiotic showed three types A,B and C garamicidin .
EFFECT OF SALINE WATER AND POTASH FERTILIZER ON PROLINE CONTENT AND WATER POTENTAL IN PISUM SATIVUM L. (VAR.SENADOR CAMBADOS)
Authors: Journal Baghdad Science
Published: March 2019

Filed experiment was conducted to test the effect of saline water and potassium fertilizers rate on proline and water potential of Pisum sativum L. (Var.Senador Cambados ) leaves . Treatments of the experiment included two levels of water salinity( 2, 7 dSm-1) as a main plot and fertilizer rates as a sub plot.
Results indicated that irrigation of plant with saline water 7 dSm-1 and fertilization 150 kg/donum increased proline accumulation and water potential 0.31 mmol/g,-17.00 bar at 9 AM morning and 0.62 mmol/g , -21.00 bar at 3 PM afternoon ,Irrigating plant with a 2 dSm-1 and fertilization 300 kg/donum decreased proline accumulation and water potential of leaves 0.22 mmol/g, -16.00 bar at 9 AM and 0.43 mmol/g,-18.00 bar at 3 PM . Irrigation plants with saline water 7 dSm-1 and fertilizer with 150 kg/ Donum K2SO4 increased Root/Shoot to 0.89 ,while 0.41 after irrigation with 2 dSm-1 saline water and fertilization with 300 kg/ Donum K2SO4 . The Na/K ratio increased to 0.53 under 7 dSm-1 of irrigation water and 150 kg/Donum fertilization and decreased to 0.1 under 2 dSm-1 irrigation water and fertilized 300 kg/ Donum . The results lead to the conclusion that potassium fertilization may reduce the inhibitory effect of increasing salinity of irrigation water on pea.
Effect of Gamma Radiation on Callus Induction and Plant Regeneration of four Wheats Triticum aestivum L. Genotypes
Authors: Journal Baghdad Science
Published: March 2019

The effect of gamma radiation at the doses (0 , 5 , 10 or 15 ) Gray on the callus of four Triticum aestivum immature embryos genotypes (AL-Hashmiya , AL-Noor AL-Zahraa and AL-Mellad ) were studied . The fresh and dry weight for callus and shoot tips beside numbers and lengths of the shoots were used as indicators after 8 weeks .

Results revealed that (AL-Noor and AL-Zahraa ) was superior by giving highest fresh and dry weight reached 274.2 and 269.2 mg and 26 and 24.3 mg respectively as compared with AL-Hashmiya and AL-Mellad. Moreover, the control treatment and the dose 10 Gray gave highest fresh weight reached 277.4 and 259.1 mg while the dry weight was highest in the control treatment and the dose 5 Gray. addition 10 Gray dose was superior to give highest rate for the number , length , fresh and dry weight of shoots reached 9.7 shoots , 3.6 cm length , 410.2 and 47.9 mg on respectively.
The Limnological Effects of River Lesser-Zab on Tigris River, Iraq
Authors: Journal Baghdad Science
Published: March 2019

The present study included the physico-chemical parameters of Lesser-Zab river and its effects on Tigris river. Monthly water samples were taken from the two rivers during January to October 1999.There were no signifcant difference in water temperatures. Both rivers were fresh to oligohalin, alkaline and very hard. Close values were determined in total suspended solids in both rivers with little increasing during rainfall period and high discharge. Water was well areated and over saturation was recorded in several occasions. Dissolved oxygen values of Tigris river were influenced by Lesser-Zab. Cations were more dominant than anions in both rivers. In Lesser-Zab, the anions were increased during spring season and declined in summer which their values influenced in Tigris river. The concentration of plant nutrients (NO2,NO3,PO4 and SiO2) were fluctuated during the study period in Lesser-Zab and their effects were cleared on Tigris river.N:P ratio values were indicated the deficiency of nitrogen in both rivers.
Effect of Some Fungal Pathogen and Some Storage Treatments on Tomato and Cucumber Fruits Under Cold Storage (8? C)
Authors: Journal Baghdad Science
Published: March 2019

In study carried out in the cold storage in college of Agric./Univ. of Baghdad at 8 ? C. shows that Alternaria , Pencillium , Rhizoctonia , Mucor , are the fungi that causes tomato fruits decay. This is the first record of Rhizoctonia and Mucor as a Tomato fruits rot under 8º c in Iraq. There is no fungal infection on cucumber fruits under 8 ? C. . Waxing tomato fruits reduced the severity of the fungi infection and gave shelflife (19 days) under 8 ? C. There is an infection with Mucor was found in tomato fruits kept in perforated polyethylene bages with 16 bores prevent the infection and the lowest severity and frequency of infection was found in waxed tomato fruits.
Part of M.Sc thesis of the Second author.
Comparative sensitivity of two freshwater snail Melanopsis nodosa and Bulinus truncatus to 2, 4-D pesticide
Authors: Journal Baghdad Science
Published: March 2019

The molluscicidal effects of herbicide 2, 4-D were studied against tow species of freshwater snail Bulinus truncatus and Melanopsis nodosa by short term experiments. Calculated values of lethal concentrations (LC50 and LC100) were maid to two spices for different period of time 24hr, 48hr, 72hr and 96hr. The study had showed that the herbicide 2, 4-D was toxic against the tow species. The toxicity of the herbicide was low or unknown in low concentrations in the first period of exposure 24and 48hr to two spices but it increase gradually with increase period of exposure. The spice of B.truncatus was more tolerant than the M.nodosa. All the individual of M nodosa was death while in B.truncatus the complete death was not appear until finish the period of exposure 96hr.
Dietary Assessment for Meals of Nurseries Children in Baghdad City
Authors: Journal Baghdad Science
Published: March 2019

The objective of this study was to assess the nutritional status of childs of nurseries in Baghdad city so that an early detection of malnutrition cases could be carried out.
The results revealed that the daily consumption of food calories, protein, fat and carbohydrate were 1180.5 calories, 27.2gm, 38gm and 180gm, respectively, which were less than the RDA values and the percentages of these nutrients supplied by the food intake were 90.8, 83.7, 87.3 and 90.3%, respectively. It was also demonstrated that the highest percentages of stunting, underweight and wasting, which amounted to 32, 22.7 and 1.5%, respectively, were among those childs who obtained inadequate calories, while the percentages of the forementioned malnutrition cases among those childs who obtained inadequate calories were 7.5, 4.5 and 3%, respectively.
The results also pointed out that the general nutritional pattern of the childs food style in this study was strictly and unbalanced due to the lack in food meals recommended to be taken daily of certain main food groups according to the food guide pyramid for young children, such as meats, poultry, fish, eggs, legumes,nuts as well as vegetables and fruits group, otherwise, the food consumed was stricted to milk,yogurt and cheese group ,vegetables and fruits group, bread, rice, and other cereals as well as their products and bakeries.
There was anumber of bad food habits among the children of research sample was also observed and was found effect the quantity and quality of food consumed.
Effect of sulphur on soil pH, growth and productivity of rape seed plant
Authors: Journal Baghdad Science
Published: March 2019

A field trial was conducted in Experimental Station of The Field Crops Department – College Of Agriculture In Abu Ghraib, University of Baghdad to assess the effect of sulphur applications and the time after application on pH and EC of soil sample solutions ,and on the growth and yield of rape seed (Brassica napus L.)A split plot design was used with four replications , The main plot included four sulphur applications levels (0,2000,3000,4000Kg S/ha) the sub plot were the time after application (0,1,2,and 3 moths) .Sulphur application significantly decreased soil pH value ,although that decrease reached minimum parameter after two months from application date .Rather increment of sulphur application level significantly raised soil EC value, So that value decreased continuously with time after the application .Sulphur application level affected significantly increased plant height/cm and decreased seed oil content percent but not affected in seed yield /plant.
Bioremoval of chromium from wastewater of tannery factory in Iraq
Authors: Journal Baghdad Science
Published: March 2019

Bioremoval of chromium from wastewater of tannery factory in Iraq was studied. The bacteria Proteus vulgaris 7E showed an enhanced capability in biosorping chromium when its concentration increased in the solution, reaching a maximum of 476,7 mg/ ml out of 492 mg/ ml under optimum conditions at pH 6 and 50°C at one hour contact time and biomass of 1 mg/ml.
The present results showed that dead cells of P. vulgaris 7E biosorbed 87.41 mg/ml of chromium in comparison with91.18 mg/ml of chromium biosorbed by living cells, this indicates the insignificant effect of physiological state of cells.
It was found that the above biosorption is physico-chemical process depends upon electrostatic attraction forces.
The results has illustrated that the most efficient eluting solution was 0.1M HCL which recovered 85% of biosorbed chromium.
P. vulgaris 7E was able to remove completely all chromium from the waste water taken from tannery factory.
Temperature Dependence of Hall Mobility AndCarrier Concentration of pb0.55S0.45 Films
Authors: Journal Baghdad Science
Published: March 2019

Measurements of Hall effect properties at different of annealing temperature have been made on polycrystalline Pb0.55S0.45 films were prepared at room temperature by thermal evaporation technique under high vacuum 4*10-5 torr . The thickness of the film was 2?m .The carrier concentration (n) was observed to decrease with increasing the annealing temperature. The Hall measurements showed that the charge carriers are electrons (i.e n-type conduction). From the observed dependence on the temperature, it is found that the Hall mobility (µH), drift velocity ( d) carrier life time ( ), mean free path (?) were increased with increasing annealing temperature
Effect of leaves Extracts of Duranta repens on growth and activity of some types of Pathogenic Bacteria and Some types of Fungi
Authors: Journal Baghdad Science
Published: March 2019

A study were conducted to examinate the effect of organic and aqueous (Hot, Cold) Extracts from leaves of Duranta repens on the growth and activities of the following types of Bacteria:- Staphylococcus aureus,Streptococcus pyogens ,Escherichia coli,Klebsilla pneumonia, in addition to the yeast Candida albicans and the fungi Aspergullis niger ,Aspergulls flavus.The result showed that gram Positive Bacteria is more sensitive to the extracts than gram negative bacteria with Minimum inhibitory concentration (MIC) value (50,25,50,100)% and Minimum Bactericidal Concentration (MBC) value (100,50,200,100)% for all types Bacteria respectively . The most active extract against A.niger ,A,flavus was cold and hot aqueous extract from the leaves with diameter growth of colony value of ( 0.93,0.37)cm for A.niger in 20 % concentration compared with organic extract (0.26)cm, and the inhibition zone value of cold and hot extract to A.flavus (0.90,0.80)cm respectively compared with organic extract (7.056)cm.
Estimation of Immunoglobulins and complements and Using Enzyme linked Immuno sorbant Assay in Identification of Vulvovaginal candidiasis
Authors: Journal Baghdad Science
Published: March 2019

This study was conducted to determine the Immuno – globulins and complements quantitatively. The result revealed that the concentration of Immunoglobulin M(IgM) was increased significantly in patient group comparing with control group . The concentration of complement protein C4 was increased significantly in patient group comparing with control group.IgG of Candida albicans was detected by using ELISA Technique, the result indicated also that this antibody was found in 628% of the women who infected with Vulvovaginal Candidiasis. The sensitivity and specificity of the test were 63% and 89% respectively.
A Study of the Nutritional Behavior and Body Mass Indexes for Students of Age (17-25) Years In Baghdad, Iraq.
Authors: Journal Baghdad Science
Published: March 2019

The aim of this study was to assess the nutritional status for samples at the age of 17-15 years. These samples were taken from secondary schools and universities in Baghdad area, 123 of them were male and 261 were female. Data on weight, height and body mass index (BMI) were determined in each individual. Smaller sample of 215 individuals (male and female) from the original sample was taken in order to record their nutritional behavior and daily food intake during the 24 hours prior to the visit through personal meeting using special questionnaire. The results showed that the weight and the height were within the range of the people of neighboring Arab countries, who are in the same age. Beside 44.4- 55.95% of these samples were within the normal weight using body mass index. Percentages of obesity and overweight were between 43.5- 6.5% for male and female respectively. There was an increase in daily food intake in general for essential diet and energy indeed, as recorded in nutritional behavior. 67% of samples have their breakfast every day. There were 51% of the samples having snacks (additional meal) between the major meals everyday and 62% have beverages every day. Also high percentage of samples were having milk and its products, vegetables, fruits (as nutritional sources) every day and the percentages were 47%, 67%, 78% respectively. In general their nutritional behavior and daily food intake were within the limits which showed by American recommended daily dietary, still there was some incorrect nutritional behavior which need more education and learning about nutrition.
Study of Stability of Personal Dosimeter
Authors: Journal Baghdad Science
Published: March 2019

The study is carried out by using personal dosimeters (film badge). The films are irradiated to absorbed dose of range (0.01-10000 rad). The calibration curves are drawn by using the ordinary method taking into account the filtration effects in three regions (D (Pb/Sn),D(Du),D(300). The calibration films are stored in ambient condition. It is found that the optical density increases, which is attributed to the photodegradation of the films may induce localized states in the energy gap causing increasing in optical absorption, but optical density decreases, which attributed to the photodegradation of the films may cause some cracks at the film surface during the first month, whereas at the rest months we see clear stability in optical density for the irradiated films. Before irradiation, there is increasing in optical density during the second month, and decreasing in optical density during the rest months. It is concluded that aging effects must be considered for personal dosimeter before and after irradiation to know its use ability as a permanent record.
Carbonization and Treatment the High Temperature Distortion of Thoriated Tungsten Cathode for High Power Electronic Tubes
Authors: Journal Baghdad Science
Published: March 2019

A metal mandrel was designed for manufacturing the cathodes of high power electron tube ( Tetrode ) used in broadcasting transmitting tubes type TH558 and CQS200.The cathodes were manufactured in the present work from thoriated tungsten wires ( 2? ThO2- W) with different diameters .These cathodes were carbonized in sequences of processes to determine the carbonization parameters (temperature, pressure, time, current and voltage).Then the carbonized cathodes dimension were accurately measured to determine the deviation due to the high temperature distortion effect at about 1800°C .the distorted cathodes due to the carbonization process was treated when it was subjected inside the vacuum chamber and heat treated again .The carbonized cathode distortions as a function of temperature were measured in the range of (1500°C-1800°C).The temperature was determined using an optical pyrometer and also calculated using Stephan's-Boltzmann relation.
Motion Recording for Surveillance Camera
Authors: Journal Baghdad Science
Published: March 2019

In this paper we present an operational computer vision system for real-time motion detection and recording that can be used in surveillance system. The system captures a video of a scene and identifies the frames that contains motion and record them in such a way that only the frames that is important to us is recorded and a report is made in the form of a movie is made and can be displayed. All parts that are captured by the camera are recorded to compare both movies. This serves as both a proof-of- concept and a verification of other existing algorithms for motion detection. Motion frames are detected using frame differencing. The results of the experiments with the system indicate the ability to minimize some of the problems false detection and missed detections (like in a sudden change of light in the scene). The software part is written in Matlab language as an M-file and using the Simulink library, the hardware part we used a Pentium 4 computer with a web camera or a laptop integrated camera.
Microbial Contamination in Some Commercial Biscuits in Baghdad City
Authors: Journal Baghdad Science
Published: March 2019

This study has been conducted to know the level of microbial ( bacteria and fungi) contamination in 5 types of biscuits from local markets of Baghdad city. Fifty samples (ten sample for each kind of biscuit) were studed,Two are local,others are Iranian,Turkish,and Holandies. The following results have been achieved :1. The highest number of bacteria was 21.6×103 cell/g in Iranian biscuit while the lowest number was 14.3× 103 cell/g in local biscuit No.1 . The highest number of fungi was 16×103 colony/g and the lowest number was 5.3×103 colony/g in the Iranian and the local biscuit No.1,respectively.2. Staphylococcus aureus was the major bacteria appeared at highest level of 100% in Turkish biscuit. The lowest percentage was found in Hollandian biscuit with 37.28%. Bacillus cereus was the major bacteria with a percentage of 100% in local biscuit No.2 where as the lowest was in local biscuit No,1with a percentage of 20.93%, while it was not existed in Turkish biscuit. Esherichia coli was found in Hollandian biscuit at highest rate of 38.98% , the lowest value was appeared in Iranian biscuit with 28.16% while it was not exited in local biscuit No.1,2 and Turkish biscuit.3. Aspergillus niger appeared at highest level of 66.66% in Hollandian biscuit, while was the lowest 37.73% in local biscuit No.1 and not existed in local biscuit No.2, The highest value of A.flavus was 69.76% in local biscuit No.2 and the lowest value in Hollandian biscuit in percentage 8.33%. It has not appeared in Iranian and Turkish biscuit. The A. terreus appeared at highest rate in Turkish biscuit with 33.33% , the lowest value was in local biscuit No.2 at 11.62% and was not appeared in Hollandian biscuit.The Penicillium spp. Was found at highest rate 25% in Hollandian biscuit , the lowest value of 9.52% was appeared in Turkish biscuit.
The effect of wastewater disposal on the water quality and phytoplankton in Erbil wastewater channel.
Authors: Journal Baghdad Science
Published: March 2019

In this study, phytoplankton density, chlorophyll-a, and selected physico- chemical parameters were investigated in Erbil wastewater channel. The surveys were carried out monthly from May 2003 to April 2004. Samplings were established on three sites from headwaters to the mouth. The results showed that pH was in alkaline side of neutrality, with significant differences (P
Extraction and Characterization of rapeseed oil (Brassca campestris ) and it's Effected on Microorganisms
Authors: Journal Baghdad Science
Published: March 2019

Oil from Brassca campestris (local variety) was extracted with hexane using Soxhlet. The extracted oil was characterized and its antimicrobial activity was determined as well.
The content of extracted oil was 40% with 0.5% of volatile oil .Oil was immiscible with polar solvent such as ethanol, acetone and water, while it was easily miscible with chloroform due to its hydrophobicity.
The result of organoleptic tests revealed that the oil is clear yellow in color and odorless with acceptable taste.
The oil was stable at 4 -25 C? for a month. Refractive index (RI) of oil was 1.4723 with density of 0.914, [both at 4-25 C?]. Boiling point 386 C?. Infra red spectroscopy (IR) indicated the presence of different chemical groups (C=C , C=O ? C-H) with different characters .Oil exhibited antimicrobial activity against Gram positive and negative bacteripa in addition to its activity against Candida albicans .
Antibacterial activity of ginger extracts and its essential oil on some of pathogenic bacteria
Authors: Journal Baghdad Science
Published: March 2019

The antimicrobial activity of ginger extracts ( cold-water, hot-water, ethanolic and essential oil ) against some of pathogenic bacteria ( Escherichia coli , Salmonella sp , Klebsiella sp , Serratia marcescens, Vibrio cholerae , Staphylococcus aureus , Streptococcus sp) was investigated using Disc diffusion method , and the results were compared with the antimicrobial activity of 12 antibiotics on the same bacteria .
The results showed that the ginger extracts were more effective on gram-positive bacteria than gram-negative . V. cholerae and S. marcescens,were the most resistant bacteria to the extracts used , while highest inhibition was noticed against Streptococcus sp (28 mm) . The ethanolic extract showed the broadest antibacterial activity ( 11 to 28 mm ) , in comparison with moderate activity of essential oil , it was observed that the cold-water extract was more effective on the bacteria than hot-water extract .
Ginger ethanolic extract presented higher diameter of inhibition zone for Streptococcus sp than in Ciprofloxacin , Cefotaxime , Cefalotin , Cephalexin and Cephaloridine , also it was found a similarity between the higher inhibition zones of ethanolic extract of ginger and some antibiotics for S. aureus , E. coli , Salmonella sp and Klebsiella sp . V. cholerae and S. marcescens,also highly resistant to antibiotics . Phytochemical analysis of ethanolic extract of ginger revealed the present of glycosides, terpenoids, flavonids and phenolic compounds
Synthesis and Characterization of New Metal Complexes of α-Aminonitriles Derived from P- Toluidine and Aromatic Aldehydes
Authors: Journal Baghdad Science
Published: March 2019

New metal complexes of some transition metal ions Co(II), Cu(II) , Cd(II) and Zn(II) were prepared by their reaction with previously prepared ligands HLI= (P-methyl anilino) phenyl acetonitrile and HLII = (P-methyl anilino) –P– chloro phenyl acetonitrile . The two ligands were prepared by Strecker’s procedure which includ the reaction of p- toluidine with benzaldehyde and P- chlorobenzaldehyde respectively. Structures were proposed depending on atomic absorption , i.r. and u.v.visible spectra in addition to magnetic susceptibility and electrical conductivity measurements.
The Effects of ? – Rays on The Optical Constants of ZnS Thin Films
Authors: Journal Baghdad Science
Published: March 2019

ZnS thin films were grown onto glass substrates by flash evaporation technique, the effects of ? – rays on the optical constants of ZnS these films were studied. It was found that ? – rays affected all the parameters under investigation.
Measuring Uranium in the Soil of Some Area in Missan Governorate/ Iraq
Authors: Journal Baghdad Science
Published: March 2019

Concentrations of uranium were measured in this study for twenty soil samples from four areas with different depths (soil surface-20-40-60-80)cm .The study regions include Missan Governorate (Al-Iskan area,Al-Shibbana area ,Hai-Al Moualimin Al Jadied area ,Sector 30 area).
The Uranium concentrations in soil samples measured by using fission tracks registration in (CR-39) track detector that caused by the bombardment of (U-283) with thermal neutrons from (241Am-Be) neutron source that has flux neutron thermal of (5 ×103 n cm-2 s-1).
The concentrations values were calculated by a comparison with standard samples. Through out the result, it was found that averages of uranium concentrations in soil samples were as the following : Al - Iskan area (0.404 ± 2.765) ppm, Al - Shibbana area (0.432 ± 1.719) ppm, Hai - Al - Moualimin Al - Jadied (0.236 ± 2.320) ppm, Sector 30 area (2.158±0.631) ppm.
Laser Effect on Fatique Resistance of Carbon Steel
Authors: Journal Baghdad Science
Published: March 2019

Laser beam has been widely used to improve the mechanical properties of the metals. It used for cutting, drilling, hardening, welding……etc. The use of Laser beam has many features in accuracy and speeding in work, also in the treatment of metals locally, and in the places that is hard to reach by traditional ways.
In this research a surface treatment was done to medium carbon steel (0.4%C) which is common kind of steel that is used in industry. Pulsing Neodymium -YAG Laser has been used and 1.06 micrometer wave length and 5 msec and the distance is about 30 centimeter between the exit area of the Laser beam from the system and the piece that treated .
We are going to check the fatigue resistance for samples that is treated by Laser beam and Scanning Electron Microscope (SEM) and also we made check for the microstructure by using the light microscope and the SEM for the breaking samples.
The results of checking showed that there is an improvement in the fatigue resistance after the treatment by the Laser beam. The results of microscope checking showed that the beginning of the failure is from the surface area and there is more than one level of the break.
Determination of environmental lead pollution in Alawi el-hilla and Bab-almudam bus stations in Baghdad city
Authors: Journal Baghdad Science
Published: March 2019

Two main grand bus national stations in Baghdad ( Alawi-alhila and Bab-almudam) were chosen as examples to determine lead effect on its drivers. This lead came from ethyl tetra lead or ethyl tri lead which used to improve gasoline quality ,so these two stations could be consider as two of the most polluted places due to gasoline burned by hundreds cars located in these stations .The determination of environmental lead pollution was measured in blood and urines drivers. Drivers were divided in two main groups due to their ages ( 30 persons in each group). The first group for men of 22-30 years old. The second group for men of 40-60 years old. Those men were carefully chosen in order to be non-fumiers or non-alcoholics persons because of the presence of lead in alcohol and cigarettes products .The results prove the presence of high pollution levels of lead in the second group : 35ug/dl in blood and 178 ug/l in urine.
Prevalence of bacteremia among children complaining different kinds of infections under 12 years old in Baghdad
Authors: Journal Baghdad Science
Published: March 2019

This study was designed to determine the percentage and the main causative agent causing bacteremia among children aged up to 12 years and complaining from different types of infections (Respiratory, intestinal, and urinary tract infection) in Baghdad. Results showed that the percentage of infection was 46.19 % the main causative agents were Enterobacteriaceae including (E.coli , Pseudomonas , Salmonella.typhi .Serratia , Enterobacter , Klebsiella )and other than Enterobacteriaceae which includes(Staph.aureus , Staph.epidermidis , Streptococcus.Pneumonia and ?-hemolytic streptococci ) .Regarding the age factor ,results showed that the highest infection rate was among the age group (1 day-12 month ) and (12 -36month ) (64.89%)and (15.95%)respectively while the lowest was in (61 month – 12 years )and (37 -60 month) (12.76%) and( 6.38) respectively .
All bacterial strains isolated from patient were submitted to sensitivity test, results showed various reactions towards different types of antibiotics used in this study.
Superconducting Properties of the (Bi0.8Pb0.2)2(Sr0.9Ba0.1)2 Ca2Cu3-x Nix O10+? System.
Authors: Journal Baghdad Science
Published: March 2019

The effect of substitution of Ni on Cu in (Bi0.8Pb0.2)2(Sr0.9Ba0.1)2 Ca2Cu3-x Nix O10+? for (x=0,0.1….1,2,3) superconductor system and sintering time has been investigated .The samples were prepared by solid-state reaction methods.
The results show that the optimum sintering temperature is equal to 850 ºC, and the sintering time is equal to 140 h. The highest transition temperature (Tc) obtained for (Bi0.8Pb0.2)2(Sr0.9Ba0.1)2 Ca2Cu3-x NixO10+? composition was 113 with x=0.8
Phase analyses of the samples by X-ray diffraction (XRD) analysis showed an orthorhombic structure with a high Tc phases (2223) as a dominant phase and low Tc phase (2212) in addition to some impurity phases.
Evaluation of Transfected HEP-2 Cell Line Using ß-Galactosidase Reporter Assay System
Authors: Journal Baghdad Science
Published: March 2019

Liposome-mediated transfection of cancer cells provide a valuable experimental technique to study cellular gene expression and may also be adapted for gene therapy studies. However, the widely recognized advantage of liposome-mediated transfection is high efficiency. Therefore, this study were performed to optimize transfection techniques in human larynx carcinoma cell line Hep-2 using the commercial synthetic lipid TransFast™ Reagent and monitoring the expression efficiency by using the pSV-?-galactosidase Control Vector which encoded ?-galactosidase, maximum transfection efficiency were achieved with TransFast™ Reagent used at the Charge ratios of 2:1 and 0.5 µg DNA/ml, this is indicate that TransFast™ Reagent can be used as an efficient transfection agent to deliver foreign DNA into human larynx carcinoma cell line Hep-2 and expression of the transgene efficiently.
Production of Slime Layer by Staphylococcus epidermidisIsolated From Corneal Infection
Authors: Journal Baghdad Science
Published: March 2019

A total of 37 Staphylococcus epidermidis isolates, isolated from corneal scraping of patients with bacterial keratitis and 20 isolates from healthy eyes (as control) (all isolates, isolated from, Ibn Al- Haietham eye hospital / Baghdad), were tested for slime production, 52.63% of all isolates were positive-slime production (23 isolates from patients and 7 isolates from controls). It was found that positive-slime producing S. epidermidis were exhibited a high resistance to antibiotics as compared to negative-slime producing isolates.
Semigroup ideal in Prime Near-Rings with Derivations
Authors: Journal Baghdad Science
Published: March 2019

In this paper we generalize some of the results due to Bell and Mason on a near-ring N admitting a derivation D , and we will show that the body of evidence on prime near-rings with derivations have the behavior of the ring. Our purpose in this work is to explore further this ring like behavior. Also, we show that under appropriate additional hypothesis a near-ring must be a commutative ring.
Modifying Hebbian Network for Text Cipher
Authors: Journal Baghdad Science
Published: March 2019

The objective of this work is to design and implement a cryptography system that enables the sender to send message through any channel (even if this channel is insecure) and the receiver to decrypt the received message without allowing any intruder to break the system and extracting the secret information.
This work modernize the feedforward neural network, so the secret message will be encrypted by unsupervised neural network method to get the cipher text that can be decrypted using the same network to get the original text.
The security of any cipher system depends on the security of the related keys (that are used by the encryption and the decryption processes) and their corresponding lengths.
In this work, the key is the final weights that are obtained from the learning process within the neural network stage, So the work can be represented as an update or development for using the neural network to enhance the security of text.
As a result for a powerful design, the resulted cipher system provides a high degree of security which satisfies the data confidentially which is the main goal of the most cryptography systems.
The genetic inhibition of some pathogenic bacterial isolates related to Enterobacteriaceae by using Different leaves extracts of Cider (Nabag) Zizyphus spina-christa
Authors: Journal Baghdad Science
Published: March 2019

The plant Zizyphus spina-christa grows wildly in the middle and southern of Iraq locally named Nabag. In this study the antibacterial activity of several different plant extract (alcoholic hot and cold extract 80%, aqueous hot and cold extract) was tested against some gram negative bacteria that related to Enterobacteriacea as follow; Pseudomonas aeruginosa, Escherchia coli Proteus mirabilis, Serratia mercesence,. Aeromonas sp, Klebsiella pneumoniae ,Shigella sp, Salmonella enteritidis (134), S. typhi(97), S. typhimurium (300) , S. typhi, . The results showed that efficient method of extract was alcoholic hot extract from other extract methods that are used in this study. The detection of active compound in crude extracts of the leaves showed positive reaction for alkaloids, flavonoides, saponin,peptides, tannins and carbohydrates , while the aqueous hot and cold extract did not give any reaction against terpenes ,resins and coumarins . Minimum inhibitory concentration (MIC) of the ethanolic hot extracts of plants was determined and the results showed that MIC of S.typhi was 25 mg / ml and 250 mg/ml aganist Klebsiella pneumoniae Serratia mercesence , Pseudomonas aeruginosa , while other isolates showed variety in their inhibitory action. the ethanolic hot extracts of plants did not show any bacteriocidal effect against all bacteria that included in this study within concentration that used except S. typhi in concentration 50 mg/ml . The effect of Sub – MIC of the ethanolic hot extract of plant on the production of some virulence factors from selective isolates S. typhi ,showed an inhibitory effect on production of H2S but no effect on others characteristic such, mannitol and glucose fermenter at 20 mg / ml . The electrophoresis of plasmid DNA isolated from bacterial cell treated with, alcoholic hot extract at sub-MIC concentrations had a curing effect on the plasmid of S. typhi .Using infrared spectroscopy spectrum indicates the possible effect of alcoholic hot extract on the conformation of the DNA molecules affecting some of its biological functions.
Synthesis , Spectroscopic and Antibacterial Studies of Zinc(II) Complexes Derived from Salicylaldehyde, Leucylalanine and Glycylglycine
Authors: Journal Baghdad Science
Published: March 2019

Two Schiff base ligands L1 and L2 have been obtained by condensation of salicylaldehyde respectively with leucylalanine and glycylglycine then their complexes with Zn(II)were prepared and characterized by elemental analyses , conductivity measurement , IR and UV-Vis .The molar conductance measurement indicated that the Zn(II) complexes are 1:1 non-electrolytes. The IR data demonstrated that the tetradentate binding of the ligands L1 and L2 . The in vitro biological screening effect of the investigated compounds have been tested against the bacterial species Staphlococcus aureus, Escherichia coil , Klebsiella pneumaniae, Proteus vulgaris and Pseudomonas aeruginosa by the disc diffusion method . A comparative study of inhibition values of the Schiff base ligands and their complexes indicated that the complexes exhibit higher antimicrobial activity than the free ligands . Zinc ions are proven to be essential for the growth-inhibitor effect. The extent of inhibition appeared to be strongly dependent on the initial cell density and on the growth medium .
The Effect of Aqueous Extract of Citrullius colocynthis Seeds on Cellular Immunity
Authors: Journal Baghdad Science
Published: March 2019

The aqueous extract of Citrullius colocynthis dried seeds (160 ?g/ml) was in vitro evaluated for its effect on phagocytic index (PI) and lymphocyte transformation index (LTI) of blood cells obtained from 30 apparently healthy blood donors (15 males and 15 females). The PI was further in vivo evaluated in cells of peritone, spleen and liver of mice treated with the extract at a dose of 0.64 mg/kg. The results revealed that in in vitro study, phagocytic cells treated with the extract showed a significant increased percentage as compared with untreated cells (60.0 vs. 44.1%). Phagocytes obtained from peritone (44.1 vs. 30.0%) and spleen (45.6 vs. 39.6 %) of treated and untreated mice behaved in a similar manner, while liver phagocytes showed no significant difference between PI of immunological function of the investigated cells, and may use as therapeutic agent. treated and untreated mice. For LTI, cultures I and II shared an approximated mean (70.0 and 68.0%, respectively), but both indices were significantly higher than the recorded LTI in culture III (54.0%). These findings suggest that the plant extract is effective in enhancing the
Unsteady Magnetohydrodynamics oscillating flow of third order fluid with central free stream velocity
Authors: Journal Baghdad Science
Published: March 2019

In this article the unsteady magnetohydrodynamics oscillating flow of third order fluid with free stream velocity is proposed. It is found that the motion equation is controlled by five dimensionless parameters namely the coecostic parameter 4, viscoelostic parameter ?,acceleration/deceleration c,suction/blowing d and material constants ? . The effect of each of these parameters upon the velocity distribution is analysised
Design of experiments and goodness-of-fit in comparison of velocity fields in curved open channel flow simulation
Authors: Gómez-Zambrano Hernán Javier , Toro-Botero F. M. , Gómez-Giraldo Andrés
Published: March 2019

In this paper, a methodology is suggested to evaluate the goodness-of-fit between observed and numerically predicted variables in curved open channel flows. The mathematical model uses the continuity and the Reynolds averaged Navier–Stokes (RANS) equations with the RNG κ-ɛ turbulence model, numerically solved with the commercial software Ansys Inc. The concept of design of experiments (DOE) together with some criteria of goodness-of-fit was used in a new coherent suggested methodology for the calibration and validation of the numerical model. The goodness-of-fit criterion was applied to secondary flow variables like helicity, intensity of the secondary current and the vortex index instead of the primary variables like the velocity field and the free surface. This methodology guarantees that the numerical model reproduces values of the response variables very near to the observed values and it is valid for the mesh independence analysis and the setup of other numerical parameters, including those related to the boundary conditions.
Investigation of the potential of buffalo and couch grasses to grow on AFIs and for removal of nutrients from paper mill wastewater
Authors: Ayres J. R. , Awad J. , Burger H. , Marzouk J. , van Leeuwen J.
Published: March 2019

The potential growth of buffalo grass (Stenotaphrum secundatum) and couch grass (Cynodon dactylon) on artificial floating islands (AFIs) and their ability to remove total nitrogen (TN) and total phosphorus (TP) from a simulated paper mill wastewater was studied. This was done to assess the potential of AFIs for removal of nutrients from aerated stabilization basins (ASBs) that had occasional growth of blue-green algae (BGA) to bloom levels. Small scale AFIs were prepared using polyethylene foam and planted with the grasses in 30 L of tested water. Trials were conducted in a plastic covered greenhouse over a three-month period where temperatures ranged from 15 to 44 °C. The results showed that both buffalo and couch grasses can adapt to planting in AFIs showing increases of 125% and 148% in wet weight, respectively. Nutrient uptake by buffalo grass and couch grass were found to be similar. Percentage uptakes of TP and TN from the synthetic water by the buffalo grass were 82% and 47%, whereas by couch grass, uptakes were 83% and 45%, respectively.
Exploiting Cancer Metal Metabolism using Anti-Cancer Metal- Binding Agents
Authors: Merlot Angelica M. , Kalinowski Danuta S. , Kovacevic Zaklina , Jansson Patric J. , Sahni Sumit , Huang Michael L.-H. , Lane Darius J.R. , Lok Hiu , Richardson Des R.
Published: March 2019

Metals are vital cellular elements necessary for multiple indispensable biological processes of living organisms, including energy transduction and cell proliferation. Interestingly, alterations in metal levels and also changes in the expression of proteins involved in metal metabolism have been demonstrated in a variety of cancers. Considering this and the important role of metals for cell growth, the development of drugs that sequester metals has become an attractive target for the development of novel anti-cancer agents. Interest in this field has surged with the design and development of new generations of chelators of the thiosemicarbazone class. These ligands have shown potent anticancer and anti-metastatic activity in vitro and in vivo. Due to their efficacy and safe toxicological assessment, some of these agents have recently entered multi-center clinical trials as therapeutics for advanced and resistant tumors. This review highlights the role and changes in homeostasis of metals in cancer and emphasizes the pre-clinical development and clinical assessment of metal ion-binding agents, namely, thiosemicarbazones, as antitumor agents.
Bone Metabolism Markers in Thalassemia Major-Induced Osteoporosis: Results from a Cross-Sectional Observational Study
Authors: Tsartsalis Athanasios N. , Lambrou George I. , Tsartsalis Dimitrios N. , Papassotiriou Ioannis , Vlachou Eugenia , Terpos Evaggelos , Chrousos George P. , Kanaka-Gantenbein Christina , Kattamis Antonis
Published: March 2019

Background:

Thalassemia major (TM) patients eventually face many new
health conditions, including endocrinopathies and low bone mineral density, usually
observed in the aging general population.

Objective:

The aim of the current study was to evaluate the biomarkers of bone
remodeling in TM patients and to compare them with both osteoporotic and healthy
population, in order to investigate the new therapeutic paths.

Methods:

Sixty-four patients with TM (32 men and 32 women) participated in the study.
The patients were evaluated with dual-energy X-ray absorptiometry (DXA) of the lumbar
spine and femoral neck and with markers of bone remodeling including receptor
activator of nuclear factor kappa-Β ligand (RANKL), osteoprotegerin (OPG), C-terminal
telopeptide (CTX), and sclerostin. Results were compared with those from 12
postmenopausal women with osteoporosis and 12 women with normal bone mineral
density.

Results:

The statistical analysis of the biochemical markers of bone metabolism
revealed overall significant differences between the three groups only for RANKL and
OPG/RANKL (p=0.049 and p=0.009). RANKL was higher and OPG/RANKL was lower in
TM patients compared to osteoporosis group.

Conclusion:

Patients with TM do not have a higher probability of suffering from
osteoporosis from the general population. However, some markers of osteoclast activity
differ between patients with TM and osteoporosis, indicating the possible differences in
terms of anti-osteoporotic treatment. The lack of significant differences among the three
groups in regards to the levels of CTX and sclerostin may indicate the potential efficacy
of the current osteoporotic treatment also for TM patients.
Development and Validation of Liquid Chromatography-mass Spectrometry Method for the Determination of Intracellular Concentration of Ginkgolide A, B, C, and Bilobalide in Transporter-Expressing Cells
Authors: Yaro Peter , Nie Jing , Xu Mingcheng , Zeng Kui , Zeng Su
Published: March 2019

Background:

Terpene lactones are major components of ginkgo biloba extract which are
used in cardiovascular and degenerative diseases. To study the involvement of transporters in the
transport/disposition of ginkgolides A, B, C, and bilobalide, a bioanalytical assay was developed by LCMS/
MS system for the quantitation of intracellular levels of terpene lactones in cells expressing organic
cation transporter 2 (OCT2).

Methods:

The assay involved an optimized simple sample handling with methyl tert-butyl ether for
liquid-liquid extraction and reconstitution in modified dissolution solution. Pretreatment of samples
with 50 μM ascorbic acid and the addition of ascorbic acid and formic acid in dissolution solution significantly
reduced matrix effect and stabilized the postpreparative samples. Separations were performed
by Zobrax RRHD column (extend-C18 1.8μm, 3.0 x 100mm) and acetonitrile gradient elution. The
analysis was carried out in the negative ion scan mode using multiple reaction monitoring.

Results:

The method was validated for linearity (concentration range of 20-5000nM), accuracy
(±13.1%), precision (<11.0%), recovery (94.31–105.9%), matrix effect (93.8-111.0%) and stability.
Finally, the method was applied in the determination of intracellular concentrations of ginkgolides A, B,
C, and bilobalide in Madin-Darby canine kidney (MDCK-mock) and MDCK-OCT2 cells in uptake
study.

Conclusion:

The developed method was successfully validated. Results suggest that OCT2 is involved
in the renal disposition of ginkgolide A, B, and bilobalide. This method would foster the study of
transport mediated activity via the interaction of ginkgolides and bilobalide with cellular systems.
Development and Validation of Novel UPLC-MS/MS Method for the Analysis of Macitentan in Pharmaceutical Formulations
Authors: Albayrak Mevlut , Atila Alptug
Published: March 2019

Introduction:

Macitentan is an endothelin receptor antagonist drug used in the treatment of pulmonary arterial hypertension.

Materials and Methods:

A new, sensitive, simple, accurate and rapid ultra-performance liquid chromatography in combination with tandem triple quadruple mass spectrometry (UPLC-MS/MS) method has been developed and validated for the determination of macitentan in pharmaceutical formulations. Macitentan and bosentan which are used as internal standard (IS) were detected using atmospheric pressure chemical ionization (APCI) in positive ion, multiple reaction monitoring (MRM) mode by monitoring mass transitions (precursor to product) m/z 589.1→203.3 and 552.6→311.5, respectively. Chromatographic separation was carried out on reverse phase C18 column (5 µm, 4.6 * 150 mm). Water containing 0.2 % acetic acid in acetonitrile (10:90, v/v) was used as the mobile phase in the isocratic elution. The system was optimized with injection volume of 10 µL, column temperature of 35 °C and flow rate of 1 mL min-1 Retention times were 1.97 min for macitentan and 1.72 min for IS.

Results and Discussion:

The calibration curve with a high correlation coefficient (0.9997) was linear range 0.5-500 ng mL-1. The lower limit of quantitation (LLOQ) and average recovery values were determined as 0.5 ng mL-1 and 99.7 %, respectively. The developed novel method has been successfully applied for the determination of macitentan in pure form and pharmaceutical formulations.

Conclusion:

The present method is the first study developed and validated for the determination of macitentan from the pharmaceutical preparations and pure form by UPLC-MS/MS method in the literature.