bluuebunny/crossref_metadata_2025 huggingface dataset:
Silver Fir (Abies alba Mill.): Review of Ecological Insights, Forest Management Strategies, and Climate Change’s Impact on European Forests
Authors: Bledý Michal , Vacek Stanislav , Brabec Pavel , Vacek Zdeněk , Cukor Jan , Černý Jakub , Ševčík Richard , Brynychová Kateřina
Published: June 2024

The silver fir (Abies alba Mill.) is among the most valuable conifers in Europe for ecological and economic reasons. Throughout the course of history, primarily in the 20th century, its share in stands has been declining due to ill-suited management practices, especially clear-cut management, air pollution (SO2 and NOX emissions), and wildlife-induced damage. This literature review compiles findings from 338 scientific papers. It describes futures for silver fir and its distribution, ecological requirements, threats and diseases, seed production and nurseries, and forest management practices with emphasis on ongoing climate change. Based on recent knowledge of fir ecology and population dynamics, small-scale shelterwood and selection management have been introduced in fir stands, which have also stabilized them. Fir is an essential species for maintaining high stability and biodiversity, especially on planosols and in waterlogged habitats. Owing to its shade tolerance and environmental plasticity, it can coexist very well with many tree species in mixtures, which can increase the productive potential of stands within the natural range in Europe. The average stand volume of mature fir stands ranges from 237–657 m3 ha−1. For its successful natural regeneration, it is essential to reduce cloven-hoofed game and thus prevent bud browsing damage. The attractiveness of fir in terms of heavy browsing is the highest of all conifers (52% damage). On the other hand, fir is a species relatively resistant to bark stripping and the spread of secondary rot compared with Norway spruce (Picea abies [L.] Karst.). Under global climate change, fir is expected to shift to higher elevations with sufficient precipitation, while in the southern part of its natural range or at lower elevations, outside water-influenced habitats, it is likely to decline. Climate change is intricately linked to the heightened prevalence of forest pathogens with significant damage potential in Europe, necessitating careful consideration and strategic adaptation within management practices of fir forests.
Blood-Chemistry Parameters Comparison among Different Age Stages of Chinese Sturgeon Acipenser sinensis
Authors: Chen Zhaowen , Zheng Yueping , Ji Kaile , Shang Yueyong , Wang Youji , Hu Menghong
Published: June 2024

The Chinese sturgeon (Acipenser sinensis), a critically endangered migratory fish native to the Yangtze River estuary, is experiencing alarming population declines. Understanding the physiological and biochemical profiles of this species is paramount for its conservation. However, due to limited sample availability, blood biochemical parameters have remained understudied. In this study, we examined blood chemistry in artificially cultured Chinese sturgeon ranging from 2 to 15 years of age. Our results revealed age-related trends: total protein (TP), albumin (ALB), globulin (GLO), total cholesterol (CHOL), high-density lipoprotein (HDL), low-density lipoprotein (LDL), estrogen (E2), testosterone (T), testosterone undecanoate (11-KT), and red blood cell count (RBC) increased with age, while glucose (GLU), uric acid (UA), and serum creatinine (CREA) decreased. Levels of C-reactive protein (CRP) declined from 3 to 7 years but rose from 8 to 15 years. Blood parameters showed stabilization with age, indicating enhanced resilience and immunity. Significant alterations in parameters at ages 2–3 and 14–15 suggest critical developmental stages. These findings are crucial for understanding sturgeon growth, development, migration, and reproduction, underscoring the necessity for targeted conservation efforts during pivotal life stages.
A Computational Approach to Understanding and Predicting the Edulcorant Profile of Glucosyl Steviol Glycosides
Authors: Zhou Zhuoyu , Li Wei , Wang Haijun , Xia Yongmei
Published: June 2024

Understanding the edulcorant profile of synthetic glucosyl steviol glycosides (GSGs) and rare natural steviol glycosides (SGs) is challenging due to their numerous species and rareness. This study developed a computational model based on the interactions of SG molecules with human sweet and bitter taste receptors (hSTR/hBTR). The models demonstrated a high correlation between the cumulative interaction energies and the perceived sweetness of SGs (R2 = 0.97), elucidating the mechanism of the diverse sweetness of SGs. It also revealed that more (within three) glucose residues at the C-13 position of the SG molecule yield stronger sweetness and weaker bitterness. Furthermore, the computational prediction was consistently validated with the known sweetness of GSG and also aligned well with that of several natural mogrosides. Thus, this model possesses a potential to predict the sweetness of SGs, GSGs, and mogrosides, facilitating the application or targeted synthesis of GSGs with desired sensory profiles.
Potential of Carbon Aerogels in Energy: Design, Characteristics, and Applications
Authors: Bari Gazi A. K. M. Rafiqul , Jeong Jae-Ho
Published: June 2024

In energy applications, the use of materials with hierarchical porous structures and large surface areas is essential for efficient charge storage. These structures facilitate rapid electron and ion transport, resulting in high power density and quick charge/discharge capabilities. Carbon-based materials are extensively utilized due to their tunable properties, including pore sizes ranging from ultra- to macropores and surface polarity. Incorporating heteroatoms such as nitrogen, oxygen, sulfur, phosphorus, and boron modifies the carbon structure, enhancing electrocatalytic properties and overall performance. A hierarchical pore structure is necessary for optimal performance, as it ensures efficient access to the material’s core. The microstructure of carbon materials significantly impacts energy storage, with factors like polyaromatic condensation, crystallite structure, and interlayer distance playing crucial roles. Carbon aerogels, derived from the carbonization of organic gels, feature a sponge-like structure with large surface area and high porosity, making them suitable for energy storage. Their open pore structure supports fast ion transfer, leading to high energy and power densities. Challenges include maintaining mechanical or structural integrity, multifunctional features, and scalability. This review provides an overview of the current progress in carbon-based aerogels for energy applications, discussing their properties, development strategies, and limitations, and offering significant guidance for future research requirements.
Association between Variants of the TRPV1 Gene and Body Composition in Sub-Saharan Africans
Authors: Giannì Maddalena , Antinucci Marco , Bertoncini Stefania , Taglioli Luca , Giuliani Cristina , Luiselli Donata , Risso Davide , Marini Elisabetta , Morini Gabriella , Tofanelli Sergio
Published: June 2024

In humans, the transient receptor potential vanilloid 1 (TRPV1) gene is activated by exogenous (e.g., high temperatures, irritating compounds such as capsaicin) and endogenous (e.g., endocannabinoids, inflammatory factors, fatty acid metabolites, low pH) stimuli. It has been shown to be involved in several processes including nociception, thermosensation, and energy homeostasis. In this study, we investigated the association between TRPV1 gene variants, sensory perception (to capsaicin and PROP), and body composition (BMI and bioimpedance variables) in human populations. By comparing sequences deposited in worldwide databases, we identified two haplotype blocks (herein referred to as H1 and H2) that show strong stabilizing selection signals (MAF approaching 0.50, Tajima’s D > +4.5) only in individuals with sub-Saharan African ancestry. We therefore studied the genetic variants of these two regions in 46 volunteers of sub-Saharan descent and 45 Italian volunteers (both sexes). Linear regression analyses showed significant associations between TRPV1 diplotypes and body composition, but not with capsaicin perception. Specifically, in African women carrying the H1-b and H2-b haplotypes, a higher percentage of fat mass and lower extracellular fluid retention was observed, whereas no significant association was found in men. Our results suggest the possible action of sex-driven balancing selection at the non-coding sequences of the TRPV1 gene, with adaptive effects on water balance and lipid deposition.
On the Impact of Geospace Weather on the Occurrence of M7.8/M7.5 Earthquakes on 6 February 2023 (Turkey), Possibly Associated with the Geomagnetic Storm of 7 November 2022
Authors: Ouzounov Dimitar , Khachikyan Galina
Published: June 2024

A joint analysis of solar wind, geomagnetic field, and earthquake catalog data showed that before the catastrophic M = 7.8 and M = 7.5 Kahramanmaras earthquake sequence on 6 February 2023, a closed strong magnetic storm occurred on 7 November 2022, SYM/H = −117 nT. The storm started at 08:04 UT. At this time, the high-latitudinal part of Turkey’s longitudinal region of future epicenters was located under the polar cusp, where the solar wind plasma would directly access the Earth’s environment. The time delay between storm onset and earthquake occurrence was ~91 days. We analyzed all seven strong (M7+) earthquakes from 1967 to 2020 to verify the initial findings. A similar pattern has been revealed for all events. The time delay between magnetic storm onset and earthquake occurrence varies from days to months. To continue these investigations, a retrospective analysis of seismic and other geophysical parameters just after preceded geomagnetic storms in the epicenter areas is desirable.
Comparative Metabolomic Fingerprinting Analysis of Tomato Fruits from Physalis Species in Mexico’s Balsas Basin
Authors: Arias-Martínez Sergio , Oyoque-Salcedo Guadalupe , Gutiérrez-Cárdenas Oscar , Oregel-Zamudio Ernesto , Torres-García Jesús
Published: June 2024

This study investigated the chemical and sensory distinctions in tomato fruits from three Physalis species (P. ixocarpa, P. angulata, and P. philadelphica) found in Michoacán, Mexico, using metabolomic fingerprinting through GC-MS analysis. The objective was to identify organoleptic differences that could influence consumer preferences, highlighting the significance of these species’ unique traits. These species represented a valuable genetic reservoir for potential hybridization or selection aimed at enhancing commercial varieties by focusing on organoleptic properties rather than traditional selection criteria like fruit size or yield. This research emphasizes the importance of preserving Mexican biodiversity and providing insights into domestication processes that prioritize flavor and sensory qualities. By analyzing metabolite profiles and their correlation with taste preferences, this study contributes to understanding how these differences could be leveraged in breeding programs to develop new tomato varieties with preferred flavors. It was suggested that variations in taste among the species are mainly due to differences in metabolite expression. This knowledge underscores the importance of organoleptic properties in the selection and domestication of edible fruits, offering a pathway toward the conservation and enhancement of tomato varieties through the exploitation of genetic diversity for organoleptic improvement.
Targeted Manipulation of Vertically Transmitted Endophytes to Confer Beneficial Traits in Grapevines
Authors: Chen Chun-Xiao , Guo Li-Rong , Wang Yu-Tao , Wen Yun , Li Yu , Lu Chun-Xi , Zhou Ping , Huang Shuang-Ye , Li Yi-Qian , Pan Xiao-Xia , Zhu Shu-Sheng , Yang Ming-Zhi
Published: June 2024

Vertically transmitted endophytes (VTEs) with multi-host-supporting functions are considered plant-acquired heritable traits, which can be manipulated to develop plants with the stable inheritance of these VTEs, defined here as ‘plant endophytic modification (PEM)’. To translate this hypothetical strategy into agricultural and horticultural practice, a PEM was carried out by introducing an anti-fungal pathogenic bacterium, Bacillus cereus (strain ID: ZX-2), into grapevine cuttings and growing the cuttings into vine plants. Bacterial strain XZ-2 is highly efficient in infecting grapevine cuttings and colonizing the interior of the infected cuttings, various parts of the subsequently established vine plants, and next year’s emerging vine shoots and leaves. Profiling the endophytic microbiota by high-throughput sequencing to the grapevines revealed that the colonization with exogenous ZX-2 slightly affected endophytic diversity, while significantly altering the composition and the predicted phenotypes of endophytic microbiota in ZX-2-modified grapevines (ZX-2mg). Most importantly, leaves (from both first- and second-year grapevines) of ZX-2mg conferred significantly higher (p < 0.001) anti-fungal pathogen abilities and a reduction in naturally occurring lesion area than leaves compared to the control grapevines. For all detected vines, a significant correlation (N = 37, r = 0.418; p < 0.01) between fungal pathogen inhibition rates and B. cereus (ZX-2) isolation rates was observed. In addition, ZX-2mg showed some growth promotion and a delay (15–20 days) in leaf abscission. The work established an alternative strategy to create plant lines with functions of specific VTEs via PEM, confirming the practical value of PEM in future organic farming systems.
The Use of Unmanned Aerial Systems for River Monitoring: A Bibliometric Analysis Covering the Last 25 Years
Authors: Pizarro Alonso , Valera-Gran Desirée , Navarrete-Muñoz Eva-María , Dal Sasso Silvano Fortunato
Published: June 2024

Cutting-edge technology for fluvial monitoring has revolutionised the field, enabling more comprehensive data collection, analysis, and interpretation. Traditional monitoring methods were limited in their spatial and temporal resolutions, but advancements in remote sensing, unmanned aerial systems (UASs), and other innovative technologies have significantly enhanced the fluvial monitoring capabilities. UASs equipped with advanced sensors enable detailed and precise fluvial monitoring by capturing high-resolution topographic data, generate accurate digital elevation models, and provide imagery of river channels, banks, and riparian zones. These data enable the identification of erosion and deposition patterns, the quantification of sediment transport, the evaluation of habitat quality, and the monitoring of river flows. The latter allows us to understand the dynamics of rivers during various hydrological events, including floods, droughts, and seasonal variations. This manuscript aims to provide an update on the main research themes and topics in the literature on the use of UASs for river monitoring. The latter is achieved through a bibliometric analysis of the publication trends and identifies the field’s key themes and collaborative networks. The bibliometric analysis shows trends in the number of publications, number of citations, top contributing countries, top publishing journals, top contributing institutions, and top authors. A total of 1085 publications on UAS monitoring in rivers are identified, published between 1999 and 2023, showing a steady annual growth rate of 24.44%. Bibliographic records are exported from the Web of Science (WoS) database using a comprehensive set of keywords. The bibliometric analysis of the raw data obtained from the WoS database is performed using the R software. The results highlight important trends and valuable insights related to the use of UASs in river monitoring, particularly in the last decade. The most frequently used author keywords outline the core themes of UASs monitoring research and highlight the interdisciplinary nature and collaborative efforts within the field. “River”, “topography”, “photogrammetry”, and “Structure-from-Motion” are the core themes of UASs monitoring research. These findings can guide future research and promote new interdisciplinary collaborations.
Influence of Environmental Risk Exposure on the Determinants of COVID-19 Booster Vaccination in an Urban Thai Population
Authors: Ounsaneha Weerawat , Laosee Orapin , Rattanapan Cheerawit
Published: June 2024

This study aimed to identify the influence of environmental risk exposure levels on the predictive factors of COVID-19 booster dose vaccination in an urban Thai population in the post-pandemic era. Six study locations, including the three provinces with the highest environmental risk levels and the three provinces with the lowest environmental risk levels, were selected by calculating the environmental risk exposure indexes. Participants from the capital district of each province were chosen via the simple random sampling technique and interviewed using a structured questionnaire. A total of 1315 individuals were included in a sample in this study, and the best predictors of booster dose vaccination were determined using multiple regression analysis. The results showed that a high level of environmental risk exposure occurred in the provinces with a high number of total days exceeding the limits set for PM10 and high rates of mortality for lung cancer. The number of COVID-19 booster vaccinations given amount to 43.4% of the population during the post-COVID-19 pandemic period. Our multivariate analysis indicated that individuals in the working age group (≥25 years old); those with higher education (diploma degree and above); full-time employment (government and private sectors); those with high monthly incomes (≥USD144.1); and those in areas with the lowest risk level of environmental exposure significantly contributed to the number of booster dose vaccinations given during the post-pandemic period. To summarize, the rate of COVID-19 booster dose vaccination acceptance in Thailand was influenced by socio-economic factors with environmental concerns. These findings improve our understating of both the global pandemic and how environmental exposure affects behavioral change patterns and could improve the effectiveness of post-pandemic management.
Understanding the Role of Extension Professionals in Public Health and One Health in Kansas
Authors: Poole Heather , Lona Antoinette , Barroga Toni Rose M. , Ghrist McKenzie , Mulcahy Ellyn R.
Published: June 2024

This study aims to understand the roles of selected extension professionals (EPs) in the field of public health and One Health and the challenges involved in performing these duties to their communities in the state of Kansas. To evaluate the role of EPs in public health and One Health, researchers interviewed nine (9) EPs following a set of structured questions. Emerging themes were extrapolated from the responses of the EPs. Researchers assigned codes for qualitative analysis and assigned themes related to public health, One Health, and effective delivery of services. Researchers identified the following themes related to the role of EPs in public health (youth development, physical activity, personal health care, proper nutrition, access to transportation), One Health (food safety and food security, environmental health, disease control and prevention) and effective delivery of services (community engagement, collaboration, challenges in implementation). The study provided an overview of the diverse roles that EPs play in public health and One Health, keys on how to engage the community effectively, and challenges in extending services to the community.
Oxidative Stress Markers in Multiple Sclerosis
Authors: Jiménez-Jiménez Félix Javier , Alonso-Navarro Hortensia , Salgado-Cámara Paula , García-Martín Elena , Agúndez José A. G.
Published: June 2024

The pathogenesis of multiple sclerosis (MS) is not completely understood, but genetic factors, autoimmunity, inflammation, demyelination, and neurodegeneration seem to play a significant role. Data from analyses of central nervous system autopsy material from patients diagnosed with multiple sclerosis, as well as from studies in the main experimental model of multiple sclerosis, experimental autoimmune encephalomyelitis (EAE), suggest the possibility of a role of oxidative stress as well. In this narrative review, we summarize the main data from studies reported on oxidative stress markers in patients diagnosed with MS and in experimental models of MS (mainly EAE), and case–control association studies on the possible association of candidate genes related to oxidative stress with risk for MS. Most studies have shown an increase in markers of oxidative stress, a decrease in antioxidant substances, or both, with cerebrospinal fluid and serum/plasma malonyl-dialdehyde being the most reliable markers. This topic requires further prospective, multicenter studies with a long-term follow-up period involving a large number of patients with MS and controls.
Specific Features of the Functional Activity of Human Adipose Stromal Cells in the Structure of a Partial Skin-Equivalent
Authors: Aleynik Diana Ya. , Charykova Irina N. , Rubtsova Yulia P. , Linkova Daria D. , Farafontova Ekaterina A. , Egorikhina Marfa N.
Published: June 2024

Mesenchymal adipose stromal cells (ASCs) are considered the most promising and accessible material for translational medicine. ASCs can be used independently or within the structure of scaffold-based constructs, as these not only ensure mechanical support, but can also optimize conditions for cell activity, as specific features of the scaffold structure have an impact on the vital activity of the cells. This manuscript presents a study of the secretion and accumulation that occur in a conditioned medium during the cultivation of human ASCs within the structure of such a partial skin-equivalent that is in contact with it. It is demonstrated that the ASCs retain their functional activity during cultivation both within this partial skin-equivalent structure and, separately, on plastic substrates: they proliferate and secrete various proteins that can then accumulate in the conditioned media. Our comparative study of changes in the conditioned media during cultivation of ASCs on plastic and within the partial skin-equivalent structure reveals the different dynamics of the release and accumulation of such secretory factors in the media under a variety of conditions of cell functioning. It is also demonstrated that the optimal markers for assessment of the ASCs’ secretory functions in the studied partial skin-equivalent structure are the trophic factors VEGF-A, HGF, MCP, SDF-1α, IL-6 and IL-8. The results will help with the development of an algorithm for preclinical studies of this skin-equivalent in vitro and may be useful in studying various other complex constructs that include ASCs.
Platelets and Neurodegenerative Diseases: Current Knowledge and Future Perspectives
Authors: Gallo Antonella , Lipari Alice , Di Francesco Silvino , Ianuà Eleonora , Liperoti Rosa , Cipriani Maria Camilla , Martone Anna Maria , De Candia Erica , Landi Francesco , Montalto Massimo
Published: June 2024

Platelets have a fundamental role in mediating hemostasis and thrombosis. However, more recently, a new idea is making headway, highlighting the importance of platelets as significant actors in modulating immune and inflammatory responses. In particular, platelets have an important role in the development of vascular amyloid-b-peptide(ab) deposits, known to play a relevant role in Alzheimer’s disease (AD) through accumulation and deposition within the frontal cortex and hippocampus in the brain. The involvement of platelets in the pathogenesis of AD opens up the highly attractive possibility of applying antiplatelet therapy for the treatment and/or prevention of AD, but conclusive results are scarce. Even less is known about the potential role of platelets in mild cognitive impairment (MCI). The aim to this brief review is to summarize current knowledge on this topic and to introduce the new perspectives on the possible role of platelet activation as therapeutic target both in AD and MCI.
Tau, Glial Fibrillary Acidic Protein, and Neurofilament Light Chain as Brain Protein Biomarkers in Cerebrospinal Fluid and Blood for Diagnosis of Neurobiological Diseases
Authors: Park Yongkyu , KC Nirajan , Paneque Alysta , Cole Peter D.
Published: June 2024

Neurological damage is the pathological substrate of permanent disability in various neurodegenerative disorders. Early detection of this damage, including its identification and quantification, is critical to preventing the disease’s progression in the brain. Tau, glial fibrillary acidic protein (GFAP), and neurofilament light chain (NfL), as brain protein biomarkers, have the potential to improve diagnostic accuracy, disease monitoring, prognostic assessment, and treatment efficacy. These biomarkers are released into the cerebrospinal fluid (CSF) and blood proportionally to the degree of neuron and astrocyte damage in different neurological disorders, including stroke, traumatic brain injury, multiple sclerosis, neurodegenerative dementia, and Parkinson’s disease. Here, we review how Tau, GFAP, and NfL biomarkers are detected in CSF and blood as crucial diagnostic tools, as well as the levels of these biomarkers used for differentiating a range of neurological diseases and monitoring disease progression. We also discuss a biosensor approach that allows for the real-time detection of multiple biomarkers in various neurodegenerative diseases. This combined detection system of brain protein biomarkers holds significant promise for developing more specific and accurate clinical tools that can identify the type and stage of human neurological diseases with greater precision.
Progressive Evolutionary Dynamics of Gene-Specific ω Led to the Emergence of Novel SARS-CoV-2 Strains Having Super-Infectivity and Virulence with Vaccine Neutralization
Authors: Maiti Amit K.
Published: June 2024

An estimation of the proportion of nonsynonymous to synonymous mutation (dn/ds, ω) of the SARS-CoV-2 genome would indicate the evolutionary dynamics necessary to evolve into novel strains with increased infection, virulence, and vaccine neutralization. A temporal estimation of ω of the whole genome, and all twenty-nine SARS-CoV-2 genes of major virulent strains of alpha, delta and omicron demonstrates that the SARS-CoV-2 genome originally emerged (ω ~ 0.04) with a strong purifying selection (ω < 1) and reached (ω ~ 0.85) in omicron towards diversifying selection (ω > 1). A marked increase in the ω occurred in the spike gene from alpha (ω = 0.2) to omicron (ω = 1.97). The ω of the replication machinery genes including RDRP, NSP3, NSP4, NSP7, NSP8, NSP10, NSP13, NSP14, and ORF9 are markedly increased, indicating that these genes/proteins are yet to be evolutionary stabilized and are contributing to the evolution of novel virulent strains. The delta-specific maximum increase in ω in the immunomodulatory genes of NSP8, NSP10, NSP16, ORF4, ORF5, ORF6, ORF7A, and ORF8 compared to alpha or omicron indicates delta-specific vulnerabilities for severe COVID-19 related hospitalization and death. The maximum values of ω are observed for spike (S), NSP4, ORF8 and NSP15, which indicates that the gene-specific temporal estimation of ω identifies specific genes for its super-infectivity and virulency that could be targeted for drug development.
Immune Checkpoint Inhibitors in Non-Small Cell Lung Cancer (NSCLC) Treatment: Quo Vadis?
Authors: Gargalionis Antonios N. , Papavassiliou Kostas A. , Papavassiliou Athanasios G.
Published: June 2024

Lung cancer has been established as the second most common cancer worldwide (most common cancer in men and second most common cancer in women) and as the leading cause of cancer morbidity among neoplasms [...]
Evaluation of Anticholinesterase Activity of the Fungicides Mefentrifluconazole and Pyraclostrobin
Authors: Kolić Dora , Šinko Goran
Published: June 2024

Triazoles are compounds with various biological activities, including fungicidal action. They became popular through cholinesterase studies after the successful synthesis of the dual binding femtomolar triazole inhibitor of acetylcholinesterase (AChE, EC 3.1.1.7) by Sharpless et al. via in situ click chemistry. Here, we evaluate the anticholinesterase effect of the first isopropanol triazole fungicide mefentrifluconazole (Ravystar®), developed to overcome fungus resistance in plant disease management. Mefentrifluconazole is commercially available individually or in a binary fungicidal mixture, i.e., with pyraclostrobin (Ravycare®). Pyraclostrobin is a carbamate that contains a pyrazole ring. Carbamates are known inhibitors of cholinesterases and the carbamate rivastigmine is already in use for the treatment of Alzheimer’s disease. We tested the type and potency of anticholinesterase activity of mefentrifluconazole and pyraclostrobin. Mefentrifluconazole reversibly inhibited human AChE and BChE with a seven-fold higher potency toward AChE (Ki = 101 ± 19 μM). Pyraclostrobin (50 μM) inhibited AChE and BChE progressively with rate constants of (t1/2 = 2.1 min; ki = 6.6 × 103 M−1 min−1) and (t1/2 = 1.5 min; ki = 9.2 × 103 M−1 min−1), respectively. A molecular docking study indicated key interactions between the tested fungicides and residues of the lipophilic active site of AChE and BChE. Additionally, the physicochemical properties of the tested fungicides were compared to values for CNS-active drugs to estimate the blood–brain barrier permeability. Our results can be applied in the design of new molecules with a lesser impact on humans and the environment.
Noradrenaline and Adrenoreceptors Are Involved in the Regulation of Prostaglandin I2 Production in the Porcine Endometrium after Experimentally Induced Inflammation
Authors: Jana Barbara , Całka Jarosław , Andronowska Aneta , Mówińska Aleksandra , Witek Krzysztof , Palus Katarzyna
Published: June 2024

Endometritis is a common disease in animals, leading to disruption of reproductive processes and economic losses. Noradrenergic control of prostaglandin (PG)I2 formation by inflamed endometrium is unknown. We determined the involvement of α1-, α2- and β-adrenoreceptors (ARs) in noradrenaline-influenced PGI synthase (PGIS) protein abundance and PGI2 release from porcine (1) endometrial explants with Escherichia coli (E. coli)-induced inflammation in vivo, and (2) E. coli lipopolysaccharide (LPS)-treated endometrial epithelial cells. Experiment 1. E. coli suspension (E. coli group) or saline (CON group) was injected into the uterine horns. In both groups, noradrenaline increased endometrial PGIS abundance and PGI2 release versus the control values, and it was higher in the E. coli group than in the CON group. In the CON group, a noradrenaline stimulating effect on both parameters takes place through α1D-, α2C- and β2-ARs. In the E. coli group, noradrenaline increased PGIS abundance and PGI2 release via α1A-, α2(B,C)- and β(1,2)-ARs, and PGI2 release also by α2A-ARs. Experiment 2. LPS and noradrenaline augmented the examined parameters in endometrial epithelial cells versus the control value. In LPS-treated cells, β(1,2)-ARs mediate in noradrenaline excitatory action on PGIS protein abundance and PGI2 release. β3-ARs also contribute to PGI2 release. Under inflammatory conditions, noradrenaline via ARs increases PGI2 synthesis and release from the porcine endometrium, including epithelial cells. Our findings suggest that noradrenaline may indirectly affect processes regulated by PGI2 in the inflamed uterus.
Priming of Immune System in Tomato by Treatment with Low Concentration of L-Methionine
Authors: Tanaka Tomoya , Fujita Moeka , Kusajima Miyuki , Narita Futo , Asami Tadao , Maruyama-Nakashita Akiko , Nakajima Masami , Nakashita Hideo
Published: June 2024

Various metabolites, including phytohormones, phytoalexins, and amino acids, take part in the plant immune system. Herein, we analyzed the effects of L-methionine (Met), a sulfur-containing amino acid, on the plant immune system in tomato. Treatment with low concentrations of Met enhanced the resistance of tomato to a broad range of diseases caused by the hemi-biotrophic bacterial pathogen Pseudomonas syringae pv. tomato (Pst) and the necrotrophic fungal pathogen Botrytis cinerea (Bc), although it did not induce the production of any antimicrobial substances against these pathogens in tomato leaf tissues. Analyses of gene expression and phytohormone accumulation indicated that Met treatment alone did not activate the defense signals mediated by salicylic acid, jasmonic acid, and ethylene. However, the salicylic acid-responsive defense gene and the jasmonic acid-responsive gene were induced more rapidly in Met-treated plants after infection with Pst and Bc, respectively. These findings suggest that low concentrations of Met have a priming effect on the phytohormone-mediated immune system in tomato.
Metabolomics Reveals Tyrosine Kinase Inhibitor Resistance-Associated Metabolic Events in Human Metastatic Renal Cancer Cells
Authors: Amaro Filipa , Carvalho Márcia , Bastos Maria de Lourdes , Guedes de Pinho Paula , Pinto Joana
Published: June 2024

The development of resistance to tyrosine kinase inhibitors (TKIs) is a major cause of treatment failure in metastatic renal cell carcinoma (mRCC). A deeper understanding of the metabolic mechanisms associated with TKI resistance is critical for refining therapeutic strategies. In this study, we established resistance to sunitinib and pazopanib by exposing a parental Caki-1 cell line to increasing concentrations of sunitinib and pazopanib. The intracellular and extracellular metabolome of sunitinib- and pazopanib-resistant mRCC cells were investigated using a nuclear magnetic resonance (NMR)-based metabolomics approach. Data analysis included multivariate and univariate methods, as well as pathway and network analyses. Distinct metabolic signatures in sunitinib- and pazopanib-resistant RCC cells were found for the first time in this study. A common metabolic reprogramming pattern was observed in amino acid, glycerophospholipid, and nicotinate and nicotinamide metabolism. Sunitinib-resistant cells exhibited marked alterations in metabolites involved in antioxidant defence mechanisms, while pazopanib-resistant cells showed alterations in metabolites associated with energy pathways. Sunitinib-resistant RCC cells demonstrated an increased ability to proliferate, whereas pazopanib-resistant cells appeared to restructure their energy metabolism and undergo alterations in pathways associated with cell death. These findings provide potential targets for novel therapeutic strategies to overcome TKI resistance in mRCC through metabolic regulation.
Suppressing Anaphase-Promoting Complex/Cyclosome–Cell Division Cycle 20 Activity to Enhance the Effectiveness of Anti-Cancer Drugs That Induce Multipolar Mitotic Spindles
Authors: Schuyler Scott C. , Chen Hsin-Yu , Chang Kai-Ping
Published: June 2024

Paclitaxel induces multipolar spindles at clinically relevant doses but does not substantially increase mitotic indices. Paclitaxel’s anti-cancer effects are hypothesized to occur by promoting chromosome mis-segregation on multipolar spindles leading to apoptosis, necrosis and cyclic-GMP-AMP Synthase–Stimulator of Interferon Genes (cGAS-STING) pathway activation in daughter cells, leading to secretion of type I interferon (IFN) and immunogenic cell death. Eribulin and vinorelbine have also been reported to cause increases in multipolar spindles in cancer cells. Recently, suppression of Anaphase-Promoting Complex/Cyclosome–Cell Division Cycle 20 (APC/C-CDC20) activity using CRISPR/Cas9 mutagenesis has been reported to increase sensitivity to Kinesin Family 18a (KIF18a) inhibition, which functions to suppress multipolar mitotic spindles in cancer cells. We propose that a way to enhance the effectiveness of anti-cancer agents that increase multipolar spindles is by suppressing the APC/C-CDC20 to delay, but not block, anaphase entry. Delaying anaphase entry in genomically unstable cells may enhance multipolar spindle-induced cell death. In genomically stable healthy human cells, delayed anaphase entry may suppress the level of multipolar spindles induced by anti-cancer drugs and lower mitotic cytotoxicity. We outline specific combinations of molecules to investigate that may achieve the goal of enhancing the effectiveness of anti-cancer agents.
Comparative Analysis of Orthosteric and Allosteric GLP-1R Agonists’ Effects on Insulin Secretion from Healthy, Diabetic, and Recovered INS-1E Pancreatic Beta Cells
Authors: Reed Joshua , Higginbotham Victoria , Bain Stephen , Kanamarlapudi Venkateswarlu
Published: June 2024

Despite the availability of different treatments for type 2 diabetes (T2D), post-diagnosis complications remain prevalent; therefore, more effective treatments are desired. Glucagon-like peptide (GLP)-1-based drugs are currently used for T2D treatment. They act as orthosteric agonists for the GLP-1 receptor (GLP-1R). In this study, we analyzed in vitro how the GLP-1R orthosteric and allosteric agonists augment glucose-stimulated insulin secretion (GSIS) and intracellular cAMP production (GSICP) in INS-1E pancreatic beta cells under healthy, diabetic, and recovered states. The findings from this study suggest that allosteric agonists have a longer duration of action than orthosteric agonists. They also suggest that the GLP-1R agonists do not deplete intracellular insulin, indicating they can be a sustainable and safe treatment option for T2D. Importantly, this study demonstrates that the GLP-1R agonists variably augment GSIS through GSICP in healthy, diabetic, and recovered INS-1E cells. Furthermore, we find that INS-1E cells respond differentially to the GLP-1R agonists depending on both glucose concentration during and before treatment and/or whether the cells have been previously exposed to these drugs. In conclusion, the findings described in this manuscript will be useful in determining in vitro how pancreatic beta cells respond to T2D drug treatments in healthy, diabetic, and recovered states.
A Dynamic Protocol to Explore NLRP3 Inflammasome Activation in Cerebral Organoids
Authors: El Soufi El Sabbagh Dana , Attisano Liliana , Andreazza Ana Cristina , Machado Alencar Kolinski
Published: June 2024

The NLRP3 inflammasome plays a crucial role in the inflammatory response, reacting to pathogen-associated molecular patterns (PAMPs) and damage-associated molecular patterns (DAMPs). This response is essential for combating infections and restoring tissue homeostasis. However, chronic activation can lead to detrimental effects, particularly in neuropsychiatric and neurodegenerative diseases. Our study seeks to provide a method to effectively measure the NLRP3 inflammasome’s activation within cerebral organoids (COs), providing insights into the underlying pathophysiology of these conditions and enabling future studies to investigate the development of targeted therapies.
Motivation and Age Revisited: The Impact of Outcome and Process Orientations on Temporal Focus in Older and Younger Adults
Authors: Imtiaz Faizan , Vaughan-Johnston Thomas , Ji Li-Jun
Published: June 2024

When engaging in a task, individuals may prioritize the task’s results (i.e., the outcome), or they may focus on the task in and of itself (i.e., the process). Previous conceptualizations of outcome and process orientations have focused on population- and context-specific motives rather than people’s general proclivity toward these orientations across diverse tasks. Using a context-generalized outcome and process measure, we examined whether older adults (Mage = 72.8) and younger adults (Mage = 18.0) varied in their outcome and process orientations, and explored how these differences were related to temporal focus. The results indicated that, compared to their younger counterparts, older adults were more process oriented, and focused more on the present. Meanwhile, younger adults were more outcome oriented and focused more on the past and the future compared to older adults. Mediation analyses further revealed that older adults were more present focused due to their heightened process orientation, while younger adults were more past and future focused due to their outcome orientation. The implications of these findings in applied settings such as the workplace, health promotion, and business are discussed.
Psychological Distress, Academic Stress, and Burnout among Saudi Undergraduate Nursing Students
Authors: Andargeery Shaherah Yousef , Taani Murad H. , Alhalwani Rania Ali , El-Gazar Heba E.
Published: June 2024

Background: There is limited evidence on the association between psychological distress, academic stress, and burnout among Saudi nursing students. Clarifying such an association is crucial to understanding the factors associated with psychological distress and developing interventions to prevent it. Aim: To explore the prevalence and association of psychological distress with academic stress and burnout among Saudi nursing students. Methods: A cross-sectional design was used, and 237 students participated from a nursing college in Riyadh, Saudi Arabia. Students’ demographics; the Depression, Anxiety, and Stress Scale; the Academic Stress Inventory; and the Maslach Burnout Inventory were used for data collection. Results: Most of the participants reported no-to-mild depression, anxiety, and stress. Stress related to studying in groups, time management, emotional exhaustion, depersonalization, and personal accomplishment were the significant predictors of psychological distress, explaining 52.1% of the variance. Conclusions: This study suggest implementing tailored mental health screenings and support services for nursing students, embedding mental health professionals in the program, and using telehealth or mobile apps for remote monitoring to ensure comprehensive care for nursing students. Future research should consider these predictors while designing strategies to decrease psychological distress among students.
Care Bundles to Improve Hemoperfusion Performance in Patients with Severe COVID-19: A Retrospective Study
Authors: Mueankwan Sirirat , Trongtrakul Konlawij , Tajarernmuang Pattraporn , Niyatiwatchanchai Nutchanok , Kusirisin Prit , Narongkiatikhun Phoom
Published: June 2024

Background/Objectives: Hemoperfusion (HP) is employed to modulate cytokine storms in severe coronavirus disease 2019 (COVID-19) patients, requiring careful attention for success and safety. Therefore, we investigated whether our care bundles could enhance HP performance. Methods: We conducted a retrospective cohort study on adult patients (≥20 years old) with severe COVID-19 pneumonia. In the first wave (Phase I), we identified HP-related issues and addressed them with care bundles in the second wave (Phase II). The care bundles included early temperature control, precise hemodynamic monitoring, and clot prevention measures for the HP membrane. The HP success rate and associated adverse events (AEs) were assessed between the two phases. Results: The study included 60 HP (HA330) sessions from 27 cases (Phase I: 21 sessions from 9 cases; Phase II: 39 sessions from 18 cases). Patient characteristics and treatments for COVID-19 were similar, except for baseline body temperature (BT) and heart rate (HR). Phase II showed a higher success rate (67% vs. 89%, p = 0.19), although it did not reach statistical significance. Phase I recorded a significantly higher frequency of AEs (3 [IQR 1, 4] events/case vs. 1 [IQR 0, 2] events/case, p = 0.014). After implementing the care bundles, hypothermia significantly decreased (78% vs. 33%, p = 0.037), with an adjusted odds ratio of 0.15; 95% CI 0.02–0.95, p = 0.044 for baseline BT. Conclusions: Further exploration with a larger sample size is required to establish the advantages of care bundles. However, the bundles’ implementation has significantly improved hypothermia prevention.
Efficacy and Safety of Immuno-Oncology Plus Tyrosine Kinase Inhibitors as Late-Line Combination Therapy for Patients with Advanced Renal Cell Carcinoma
Authors: Hamamoto Shuzo , Tasaki Yoshihiko , Morikawa Toshiharu , Naiki Taku , Etani Toshiki , Taguchi Kazumi , Iwatsuki Shoichiro , Unno Rei , Takeda Tomoki , Nagai Takashi , Kawase Kengo , Mimura Yoshihisa , Sugiyama Yosuke , Okada Atsushi , Furukawa-Hibi Yoko , Yasui Takahiro
Published: June 2024

Background/Objectives: Immuno-oncology plus tyrosine kinase inhibitor (IO+TKI) combination therapy is an essential first-line therapy for advanced renal cell carcinoma (RCC). However, reports of its efficacy and safety as late-line therapy are lacking. This study aimed to examine the efficacy and safety of IO+TKI combination therapy as a late-line therapy for patients with RCC. Methods: We retrospectively examined 17 patients with RCC who received IO+TKI combination therapy as a second-line therapy or beyond (pembrolizumab plus axitinib, n = 10; avelumab plus axitinib, n = 5; nivolumab plus cabozantinib, n = 2). Results: The overall response and disease control rates of IO+TKI combination therapy were 29.4% and 64.7%, respectively. The median overall survival was not attained. Progression-free survival was 552 days, and 94.1% of patients (n = 16) experienced adverse effects (AEs) of any grade; moreover, 41.2% of patients (n = 7) experienced grade ≥ 3 immuno-related AEs. Conclusions: IO+TKI combination therapy may be a late-line therapy option for RCC.
Maritime Autonomous Surface Ships
Authors: Xu Haitong , Moreira Lúcia , Xiang Xianbo , Guedes Soares C.
Published: June 2024

The maritime industry faces many pressing challenges due to increasing environmental and safety regulations and crew safety concerns [...]
Sonar Fish School Detection and Counting Method Based on Improved YOLOv8 and BoT-SORT
Authors: Xing Bowen , Sun Min , Liu Zhenchong , Guan Lianwu , Han Jitao , Yan Chuanxu , Han Chuang
Published: June 2024

Fish object detection and counting in pelagic fisheries face many challenges in complex environments. Sonar imaging technology offers a solution because it generates high-resolution images underwater. In this paper, we propose a sonar-based fish object detection and counting method using an improved YOLOv8 combined with BoT-SORT to address issues such as missed detection, false detection, and low accuracy caused by complex factors such as equipment motion, light changes, and background noise in pelagic environments. The algorithm utilizes the techniques of lightweight upsampling operator CARAFE, generalized feature pyramid network GFPN, and partial convolution. It integrates with the BoT-SORT tracking algorithm to propose a new region detection method that detects and tracks the schools of fish, providing stable real-time fish counts in the designated area. The experimental results indicate that while focusing on maintaining a lightweight design, the improved algorithm achieved a 3.8% increase in recall and a 2.4% increase in mAP0.5 compared to the original algorithm. This significantly impacts scientific and rational fishery planning, marine resource protection, and improved productivity. At the same time, it provides important data support for marine ecological monitoring, environmental protection, and fishery management, contributing to sustainable fishery development and marine ecology preservation.