bluuebunny/crossref_metadata_2025 huggingface dataset:
Wisdom of crowds benefits perceptual decision making across difficulty levels
Authors: Saha Roy Tiasha , Mazumder Satyaki , Das Koel
Published: January 2021

Decades of research on collective decision making has claimed that aggregated judgment of multiple individuals is more accurate than expert individual judgement. A longstanding problem in this regard has been to determine how decisions of individuals can be combined to form intelligent group decisions. Our study consisted of a random target detection task in natural scenes, where human subjects (18 subjects, 7 female) detected the presence or absence of a random target as indicated by the cue word displayed prior to stimulus display. Concurrently the neural activities (EEG signals) were recorded. A separate behavioural experiment was performed by different subjects (20 subjects, 11 female) on the same set of images to categorize the tasks according to their difficulty levels. We demonstrate that the weighted average of individual decision confidence/neural decision variables produces significantly better performance than the frequently used majority pooling algorithm. Further, the classification error rates from individual judgement were found to increase with increasing task difficulty. This error could be significantly reduced upon combining the individual decisions using group aggregation rules. Using statistical tests, we show that combining all available participants is unnecessary to achieve minimum classification error rate. We also try to explore if group aggregation benefits depend on the correlation between the individual judgements of the group and our results seem to suggest that reduced inter-subject correlation can improve collective decision making for a fixed difficulty level.
CYP3A4 mediated pharmacokinetics drug interaction potential of Maha-Yogaraj Gugglu and E, Z guggulsterone
Authors: Sabarathinam Sarvesh , Rajappan Chandra Satish Kumar , Thangavel Mahalingam Vijayakumar
Published: January 2021

Maha yogaraja guggulu (MYG) is a classical herbomineral polyherbal formulation being widely used since centuries. The aim of this study was to investigate the effect of MYG formulation and its major constituents E & Z guggulsterone on CYP3A4 mediated metabolism. In vitro inhibition of MYG and Guggulsterone isomers on CYP3A4 was evaluated by high throughput fluorometric assay. Eighteen Adult male Sprague–Dawley rats (200 ± 25 g body weight) were randomly divided into three groups. Group A, Group B and Group C were treated with placebo, MYG and Standard E & Z guggulsterone for 14 days respectively by oral route. On 15th day, midazolam (5 mg/kg) was administered orally to all rats in each group. Blood samples (0.3 mL) were collected from the retro orbital vein at 0.25, 0.5, 0.75, 1, 2, 4, 6, 12 and 24 h of each rat were collected. The findings from the in vitro & in vivo study proposed that the MYG tablets and its guggulsterone isomers have drug interaction potential when consumed along with conventional drugs which are CYP3A4 substrates. In vivo pharmacokinetic drug interaction study of midazolam pointed out that the MYG tablets and guggulsterone isomers showed an inhibitory activity towards CYP3A4 which may have leads to clinically significant interactions.
Novel approach to enhance coastal habitat and biotope mapping with drone aerial imagery analysis
Authors: Monteiro João Gama , Jiménez Jesús L. , Gizzi Francesca , Přikryl Petr , Lefcheck Jonathan S. , Santos Ricardo S. , Canning-Clode João
Published: January 2021

Understanding the complex factors and mechanisms driving the functioning of coastal ecosystems is vital towards assessing how organisms, ecosystems, and ultimately human populations will cope with the ecological consequences of natural and anthropogenic impacts. Towards this goal, coastal monitoring programs and studies must deliver information on a range of variables and factors, from taxonomic/functional diversity and spatial distribution of habitats, to anthropogenic stress indicators such as land use, fisheries use, and pollution. Effective monitoring programs must therefore integrate observations from different sources and spatial scales to provide a comprehensive view to managers. Here we explore integrating aerial surveys from a low-cost Remotely Piloted Aircraft System (RPAS) with concurrent underwater surveys to deliver a novel approach to coastal monitoring. We: (i) map depth and substrate of shallow rocky habitats, and; (ii) classify the major biotopes associated with these environmental axes; and (iii) combine data from i and ii to assess the likely distribution of common sessile organismal assemblages over the survey area. Finally, we propose a general workflow that can be adapted to different needs and aerial platforms, which can be used as blueprints for further integration of remote-sensing with in situ surveys to produce spatially-explicit biotope maps.
Targeted sequencing to identify genetic alterations and prognostic markers in pediatric T-cell acute lymphoblastic leukemia
Authors: Chang Ya-Hsuan , Yu Chih-Hsiang , Jou Shiann-Tarng , Lin Chien-Yu , Lin Kai-Hsin , Lu Meng-Yao , Wu Kang-Hsi , Chang Hsiu-Hao , Lin Dong-Tsamn , Lin Shu-Wha , Chen Hsuan-Yu , Yang Yung-Li
Published: January 2021

T-cell acute lymphoblastic leukemia (T-ALL) is caused by the accumulation of multiple genetic alterations. To determine the frequency of common genetic mutations and possible prognostic markers in childhood T-ALL, we performed targeted sequencing of 67 genes across 64 cases treated according to Taiwan Pediatric Oncology Group protocols between January 2002 and December 2015. Together, 302 variants were identified in 60 genes including 233 single nucleotide variants and 69 indels. Sixty-four samples had a median number of six genetic lesions each (range 1–17). Thirteen genes had mutation frequencies > 10%, and 5 were > 20%, with the highest being NOTCH1 (70.31%). Protocadherins FAT1 (32.81%) and FAT3 (17.19%), and the ubiquitin ligase component FBXW7 (28.13%) had higher mutation frequencies than previously reported. Other mutation frequencies (PHF6, DNM2, DNMT3A, CNOT3, and WT1) were within previously reported ranges. Three epigenetic-related genes (KMT2D, DNMT3A, and EZH2) were mutated in our cohort. JAK-STAT signaling pathway genes had mutation frequencies of 3–13% and were observed in 23 cases (35.94%). Changes to genes in the ErbB signaling pathway were detected in 20 cases (31.25%). Patients with NOTCH1/FBXW7 mutations and RAS/PTEN germline exhibited better 5-year overall survival rates.
Higher social tolerance in wild versus captive common marmosets: the role of interdependence
Authors: de Oliveira Terceiro Francisco Edvaldo , Arruda Maria de Fátima , van Schaik Carel P. , Araújo Arrilton , Burkart Judith Maria
Published: January 2021

Social tolerance in a group reflects the balance between within-group competition and interdependence: whereas increased competition leads to a reduction in social tolerance, increased interdependence increases it. Captivity reduces both feeding competition and interdependence and can therefore affect social tolerance. In independently breeding primates, social tolerance has been shown to be higher in captivity, indicating a strong effect of food abundance. It is not known, however, how social tolerance in cooperative breeders, with their much higher interdependence, responds to captivity. Here, we therefore compared social tolerance between free-ranging and captive groups in the cooperatively breeding common marmoset and found higher social tolerance (measured as proximity near food, co-feeding, and food sharing) in the wild. Most likely, social tolerance in the wild is higher because interdependence is particularly high in the wild, especially because infant care is more costly there than in captivity. These results indicate that the high social tolerance of these cooperative breeders in captivity is not an artefact, and that captive data may even have underestimated it. They may also imply that the cooperative breeding and foraging of our hominin ancestors, which relied on strong interdependence at multiple levels, was associated with high social tolerance.
Mood alterations in mouse models of Spinocerebellar Ataxia type 1
Authors: Asher Melissa , Rosa Juao-Guilherme , Cvetanovic Marija
Published: January 2021

Spinocerebellar ataxia type 1 (SCA1) is a fatal neurodegenerative disease caused by abnormal expansion of glutamine-encoding CAG repeats in the Ataxin-1 (ATXN1) gene. SCA1 is characterized by progressive motor deficits, cognitive decline, and mood changes including anxiety and depression, with longer number of repeats correlating with worse disease outcomes. While mouse models have been very useful in understanding etiology of ataxia and cognitive decline, our understanding of mood symptoms in SCA1 has lagged. It remains unclear whether anxiety or depression stem from an underlying brain pathology or as a consequence of living with an untreatable and lethal disease. To increase our understanding of the etiology of SCA1 mood alterations, we used the elevated-plus maze, sucrose preference and forced swim tests to assess mood in four different mouse lines. We found that SCA1 knock-in mice exhibit increased anxiety that correlated with the length of CAG repeats, supporting the idea that underlying brain pathology contributes to SCA1-like anxiety. Additionally, our results support the concept that increased anxiety is caused by non-cerebellar pathology, as Purkinje cell specific SCA1 transgenic mice exhibit decreased anxiety-like behavior. Regarding the molecular mechanism, partial loss of ATXN1 may play a role in anxiety, based on our results for Atxn1 haploinsufficient and null mice.
Diversity unearthed by the estimated molecular phylogeny and ecologically quantitative characteristics of uncultured Ehrlichia bacteria in Haemaphysalis ticks, Japan
Authors: Su Hongru , Onoda Eri , Tai Hitoshi , Fujita Hiromi , Sakabe Shigetoshi , Azuma Kentaro , Akachi Shigehiro , Oishi Saori , Abe Fuyuki , Ando Shuji , Ohashi Norio
Published: January 2021

Ehrlichia species are obligatory intracellular bacteria transmitted by arthropods, and some of these species cause febrile diseases in humans and livestock. Genome sequencing has only been performed with cultured Ehrlichia species, and the taxonomic status of such ehrlichiae has been estimated by core genome-based phylogenetic analysis. However, many uncultured ehrlichiae exist in nature throughout the world, including Japan. This study aimed to conduct a molecular-based taxonomic and ecological characterization of uncultured Ehrlichia species or genotypes from ticks in Japan. We first surveyed 616 Haemaphysalis ticks by p28-PCR screening and analyzed five additional housekeeping genes (16S rRNA, groEL, gltA, ftsZ, and rpoB) from 11 p28-PCR-positive ticks. Phylogenetic analyses of the respective genes showed similar trees but with some differences. Furthermore, we found that V1 in the V1–V9 regions of Ehrlichia 16S rRNA exhibited the greatest variability. From an ecological viewpoint, the amounts of ehrlichiae in a single tick were found to equal approx. 6.3E+3 to 2.0E+6. Subsequently, core-partial-RGGFR-based phylogenetic analysis based on the concatenated sequences of the five housekeeping loci revealed six Ehrlichia genotypes, which included potentially new Ehrlichia species. Thus, our approach contributes to the taxonomic profiling and ecological quantitative analysis of uncultured or unidentified Ehrlichia species or genotypes worldwide.
Effects of marital status on overall and cancer-specific survival in laryngeal cancer patients: a population-based study
Authors: Ding Zhao , Yu Deshun , Li Hefeng , Ding Yueming
Published: January 2021

Marital status has long been recognized as an important prognostic factor for many cancers, however its’ prognostic effect for patients with laryngeal cancer has not been fully examined. We retrospectively analyzed 8834 laryngeal cancer patients in the Surveillance Epidemiology and End Results database from 2004 to 2010. Patients were divided into four groups: married, widowed, single, and divorced/separated. The difference in overall survival (OS) and cancer-specific survival (CSS) of the various marital subgroups were calculated using the Kaplan–Meier curve. Multivariate Cox regression analysis screened for independent prognostic factors. Propensity score matching (PSM) was also conducted to minimize selection bias. We included 8834 eligible patients (4817 married, 894 widowed, 1732 single and 1391 divorced/separated) with laryngeal cancer. The 5-year OS and CSS of married, widowed, single, and separated/divorced patients were examined. Univariate and multivariate analyses found marital status to be an independent predictor of survival. Subgroup survival analysis showed that the OS and CSS rates in widowed patients were always the lowest in the various American Joint Committee on Cancer stages, irrespective of sex. Widowed patients demonstrated worse OS and CSS in the 1:1 matched group analysis. Among patients with laryngeal cancer, widowed patients represented the highest-risk group, with the lowest OS and CSS.
Wastewater COD characterization: RBCOD and SBCOD characterization analysis methods
Authors: Zhang Jingbing , Shao Yuting , Liu Guohua , Qi Lu , Wang Hongchen , Xu Xianglong , Liu Shuai
Published: January 2021

Wastewater characterization is the basis for process design and operation optimization of wastewater treatment plants (WWTPs). In this work, a comprehensive study of the respirometry method has been performed to evaluate the biodegradable organic matters of wastewater. First, the optimal initial substrate to biomass ratio (S0/X0) was confirmed. Second, under the optimal S0/X0, the degradation curves of wastewater carbon oxygen demand (COD) components rapidly biodegradable COD (RBCOD) and slowly biodegradable COD (SBCOD) were obtained. Third, the Mann–Kendall test was performed to confirm the time point (t2) when endogenous respiration levels were reached, and the hydrolysis model was used to determine the time point (t1) of the SBCOD degradation stage. Considering the results, an adequate wastewater COD characterization method for RBCOD and SBCOD has been proposed. This study provides strong support to carry out effective and feasible process design, process diagnosis and optimization capability, can help achieve refined and stable operational management of WWTPs.
Application of wood ash leads to strong vertical gradients in soil pH changing prokaryotic community structure in forest top soil
Authors: Bang-Andreasen Toke , Peltre Mette , Ellegaard-Jensen Lea , Hansen Lars Hestbjerg , Ingerslev Morten , Rønn Regin , Jacobsen Carsten Suhr , Kjøller Rasmus
Published: January 2021

Wood ash is alkaline and contains base-cations. Application of wood ash to forests therefore counteracts soil acidification and recycle nutrients removed during harvest. Wood ash application to soil leads to strong vertical gradients in physicochemical parameters. Consequently, we designed an experimental system where small-scale vertical changes in soil properties and prokaryotic community structure could be followed after wood ash application. A mixed fly and bottom ash was applied in dosages of 3 and 9 t ha−1 to the surface of soil mesocosms, simulating a typical coniferous podzol. Soil pH, exchangeable cations and 16S prokaryotic community was subsequently assessed at small depth intervals to 5 cm depth at regular intervals for one year. Wood ash significantly changed the prokaryotic community in the top of the soil column. Also, the largest increases in pH and concentrations of exchangeable cations was found here. The relative abundance of prokaryotic groups directionally changed, suggesting that wood ash favors copiotrophic prokaryotes at the expense of oligotrophic and acidophilic taxa. The effect of wood ash were negligible both in terms of pH- and biological changes in lower soil layers. Consequently, by micro-vertical profiling we showed that wood ash causes a steep gradient of abiotic factors driving biotic changes but only in the top-most soil layers.
Effect of biomass immobilization and reduced graphene oxide on the microbial community changes and nitrogen removal at low temperatures
Authors: Banach-Wiśniewska Anna , Tomaszewski Mariusz , Hellal Mohamed S. , Ziembińska-Buczyńska Aleksandra
Published: January 2021

The slow growth rate and high optimal temperatures for the anaerobic ammonium oxidation (anammox) bacteria are significant limitations of the anammox processes application in the treatment of mainstream of wastewater entering wastewater treatment plant (WWTP). In this study, we investigate the nitrogen removal and microbial community changes in sodium alginate (SA) and sodium alginate–reduced graphene oxide (SA-RGO) carriers, depending on the process temperature, with a particular emphasis on the temperature close to the mainstream of wastewater entering the WWTP. The RGO addition to the SA matrix causes suppression of the beads swelling, which intern modifies the mechanical properties of the gel beads. The effect of the temperature drop on the nitrogen removal rate was reduced for biomass entrapped in SA and SA-RGO gel beads in comparison to non-immobilized biomass, this suggests a ‘‘protective” effect caused by immobilization. However, analyses performed using next-generation sequencing (NGS) and qPCR revealed that the microbial community composition and relative gene abundance changed significantly, after the implementation of the new process conditions. The microbial community inside the gel beads was completely remodelled, in comparison with inoculum, and denitrification contributed to the nitrogen transformation inside the beads.
Evaluation of a multiparametric MRI radiomic-based approach for stratification of equivocal PI-RADS 3 and upgraded PI-RADS 4 prostatic lesions
Authors: Brancato Valentina , Aiello Marco , Basso Luca , Monti Serena , Palumbo Luigi , Di Costanzo Giuseppe , Salvatore Marco , Ragozzino Alfonso , Cavaliere Carlo
Published: January 2021

Despite the key-role of the Prostate Imaging and Reporting and Data System (PI-RADS) in the diagnosis and characterization of prostate cancer (PCa), this system remains to be affected by several limitations, primarily associated with the interpretation of equivocal PI-RADS 3 lesions and with the debated role of Dynamic Contrast Enhanced-Magnetic Resonance Imaging (DCE-MRI), which is only used to upgrade peripheral PI-RADS category 3 lesions to PI-RADS category 4 if enhancement is focal. We aimed at investigating the usefulness of radiomics for detection of PCa lesions (Gleason Score ≥ 6) in PI-RADS 3 lesions and in peripheral PI-RADS 3 upgraded to PI-RADS 4 lesions (upPI-RADS 4). Multiparametric MRI (mpMRI) data of patients who underwent prostatic mpMRI between April 2013 and September 2018 were retrospectively evaluated. Biopsy results were used as gold standard. PI-RADS 3 and PI-RADS 4 lesions were re-scored according to the PI-RADS v2.1 before and after DCE-MRI evaluation. Radiomic features were extracted from T2-weighted MRI (T2), Apparent diffusion Coefficient (ADC) map and DCE-MRI subtracted images using PyRadiomics. Feature selection was performed using Wilcoxon-ranksum test and Minimum Redundancy Maximum Relevance (mRMR). Predictive models were constructed for PCa detection in PI-RADS 3 and upPI-RADS 4 lesions using at each step an imbalance-adjusted bootstrap resampling (IABR) on 1000 samples. 41 PI-RADS 3 and 32 upPI-RADS 4 lesions were analyzed. Among 293 radiomic features, the top selected features derived from T2 and ADC. For PI-RADS 3 stratification, second order model showed higher performances (Area Under the Receiver Operating Characteristic Curve—AUC— = 80%), while for upPI-RADS 4 stratification, first order model showed higher performances respect to superior order models (AUC = 89%). Our results support the significant role of T2 and ADC radiomic features for PCa detection in lesions scored as PI-RADS 3 and upPI-RADS 4. Radiomics models showed high diagnostic efficacy in classify PI-RADS 3 and upPI-RADS 4 lesions, outperforming PI-RADS v2.1 performance.
Clinically relevant variants in a large cohort of Indian patients with Marfan syndrome and related disorders identified by next-generation sequencing
Authors: Nayak Shalini S. , Schneeberger Pauline E. , Patil Siddaramappa J. , Arun Karegowda M. , Suresh Pujar V. , Kiran Viralam S. , Siddaiah Sateesh , Maiya Shreesha , Venkatachalagupta Shrikanth K. , Kausthubham Neethukrishna , Kortüm Fanny , Rau Isabella , Wey-Fabrizius Alexandra , Van Den Heuvel Lotte , Meester Josephina , Van Laer Lut , Shukla Anju , Loeys Bart , Girisha Katta M. , Kutsche Kerstin
Published: January 2021

Marfan syndrome and related disorders are a group of heritable connective tissue disorders and share many clinical features that involve cardiovascular, skeletal, craniofacial, ocular, and cutaneous abnormalities. The majority of affected individuals have aortopathies associated with early mortality and morbidity. Implementation of targeted gene panel next-generation sequencing in these individuals is a powerful tool to obtain a genetic diagnosis. Here, we report on clinical and genetic spectrum of 53 families from India with a total of 83 patients who had a clinical diagnosis suggestive of Marfan syndrome or related disorders. We obtained a molecular diagnosis in 45/53 (85%) index patients, in which 36/53 (68%) had rare variants in FBN1 (Marfan syndrome; 63 patients in total), seven (13.3%) in TGFBR1/TGFBR2 (Loeys–Dietz syndrome; nine patients in total) and two patients (3.7%) in SKI (Shprintzen–Goldberg syndrome). 21 of 41 rare variants (51.2%) were novel. We did not detect a disease-associated variant in 8 (15%) index patients, and none of them met the Ghent Marfan diagnostic criteria. We found the homozygous FBN1 variant p.(Arg954His) in a boy with typical features of Marfan syndrome. Our study is the first reporting on the spectrum of variants in FBN1, TGFBR1, TGFBR2, and SKI in Indian individuals.
Increased risk of major depressive disorder in sleep apnea patients in Taiwan
Authors: Chen Chia-Min , Kuo Chia-Yu , Wu Meng-Ni , Hung Jen-Yu , Hsu Chung-Yao , Tsai Ming-Ju
Published: January 2021

The association between sleep apnea (SA) and depression had been reported in a few previous studies. However, whether SA increases the risk of major depressive disorder (MDD) has not been studied comprehensively in a large-scale study. We performed this population-based cohort study to assess the association between SA and MDD. We identified adult patients having SA from the Taiwan National Health Insurance Research Database and excluded those having MDD before SA diagnosis. Thirty control subjects were randomly selected to match to each SA patient by age and sex. Totally, 10,259 SA patients were matched to 102,590 control subjects. The incidence rate and cumulative incidence of MDD were significantly higher in SA patients than in the control subjects (both p < 0.0001). Multivariable Cox regression analysis showed that SA remained an independent risk factor for incident MDD after adjusting for age, sex, residency, income level, and comorbidities (hazard ratio = 2.9 [95% CI 2.8–3.1], p < 0.0001). In summary, SA patients have an increased risk to develop MDD. Physicians caring for SA patients must pay attention to their psychosocial health status.
Emission characteristics variation of GaAs0.92Sb0.08/Al0.3Ga0.7As strained multiple quantum wells caused by rapid thermal annealing
Authors: Wang Dengkui , Gao Xian , Tang Jilong , Fang Xuan , Fang Dan , Wang Xinwei , Lin Fengyuan , Wang Xiaohua , Chen Rui , Wei Zhipeng
Published: January 2021

Rapid thermal annealing is an effective way to improve the optical properties of semiconductor materials and devices. In this paper, the emission characteristics of GaAs0.92Sb0.08/Al0.3Ga0.7As multiple quantum wells, which investigated by temperature-dependent photoluminescence, are adjusted through strain and interfacial diffusion via rapid thermal annealing. The light-hole (LH) exciton emission and the heavy-hole (HH) exciton emission are observed at room temperature. After annealing, the LH and HH emission peaks have blue shift. It can be ascribed to the variation of interfacial strain at low annealing temperature and the interfacial diffusion between barrier layer and well layer at high annealing temperature. This work is of great significance for emission adjustment of strained multiple quantum wells.
Introducing winter rice cropping by using non-saline tidal water influx in western basins of South 24 Parganas, India
Authors: Mukherjee Prasun , Das Subhasish , Mazumdar Asis
Published: January 2021

A population exceeding 3.8 million people in the western region of 24-Parganas (South) is directly or indirectly reliant on agriculture as their primary source of livelihood. The agricultural trend shows a clear lack of multi-cropping with a drop of nearly 30% in rice cultivation during the winter season. Nearly 50% of the region is directly dependent on canals. The introduction of tidal water in the canal network provides an exceptionally economical and highly effective mode of irrigation water supply. The primary aim of the study was to identify the cartographic characteristics and channel hydraulics in the summer season. It was noted that the canals have a wide discharge range of 0.03–540.03 m3/s, average evaporation loss of 9.07 mm/day with a seepage loss ranging from 0.04 to 6.36 m3/s. The tidal water ingress quantity was calculated to be 4.17 Mm3, 5.32 Mm3, 1.88 Mm3 at Diamond Harbour sluice (Sl.), Kulpi Sl. and Kholakhali Sl. respectively. It was denoted that the augmentation of tidal backwater six times monthly would suffice the winter crop water requirement for the majority of the basins. This would result in the production of 172.13 kt which was previously 17.6 kt resulting in an increase of production by 878.01%. The per capita income would also be increased by nearly 978% for the season, resulting in the macro-socioeconomic upliftment of the region.
Supercapacitors based on Ti3C2Tx MXene extracted from supernatant and current collectors passivated by CVD-graphene
Authors: Kumar Sunil , Rehman Malik Abdul , Lee Sungwon , Kim Minwook , Hong Hyeryeon , Park Jun-Young , Seo Yongho
Published: January 2021

An ultrahigh capacity supercapacitor is fabricated using a nano-layered MXene as an active electrode material, and Ni-foil is used as a current collector. The high-quality Ti3C2Tx obtained from supernatant during etching and washing processes improves the specific capacitance significantly. As another strategy, the surface of Ni-foil is engineered by coating chemical vapor deposition-grown graphene. The graphene grown directly on the Ni-foil is used as a current collector, forming the electrode structure of Ti3C2Tx/graphene/Ni. The surface passivation of the current collectors has a high impact on charge-transfer, which in turn increases the capacitance of the supercapacitors. It is found that the capacitance of the graphene-based supercapacitors is more than 1.5 times of the capacitance without graphene. A high specific capacitance, ~ 542 F/g, is achieved at 5 mV/s scan rate based on cyclic voltammetry analysis. Also, the graphene-based supercapacitor exhibits a quasi-rectangular form in cyclic voltammetry curves and a symmetric behavior in charge/discharge curves. Furthermore, cyclic stability up to 5000 cycles is confirmed with high capacitance retention at high scan rate 1000 mV/s. A reduced series resistance with a high limit capacitance is revealed by equivalent circuit analysis with the Nyquist plot.
Effects of aerobic, resistance and concurrent exercise on pulse wave reflection and autonomic modulation in men with elevated blood pressure
Authors: Farinatti Paulo , da Silva Itaborahy Alex , de Paula Tainah , Monteiro Walace David , Neves Mário F.
Published: January 2021

The acute effects of exercise modes on pulse wave reflection (PWR) and their relationship with autonomic control remain undefined, particularly in individuals with elevated blood pressure (BP). We compared PWR and autonomic modulation after acute aerobic (AE), resistance (RE), and concurrent exercise (CE) in 15 men with stage-1 hypertension (mean ± SE: 34.7 ± 2.5 years, 28.4 ± 0.6 kg/m2, 133 ± 1/82 ± 2 mmHg). Participants underwent AE, RE, and CE on different days in counterbalanced order. Applanation tonometry and heart rate variability assessments were performed before and 30-min postexercise. Aortic pressure decreased after AE (− 2.4 ± 0.7 mmHg; P = 0.01), RE (− 2.2 ± 0.6 mmHg; P = 0.03), and CE (− 3.1 ± 0.5 mmHg; P = 0.003). Augmentation index remained stable after RE, but lowered after AE (− 5.1 ± 1.7%; P = 0.03) and CE (− 7.6 ± 2.4% P = 0.002). Systolic BP reduction occurred after CE (− 5.3 ± 1.9 mmHg). RR-intervals and parasympathetic modulation lowered after all conditions (~ 30–40%; P < 0.05), while the sympathovagal balance increased after RE (1.2 ± 0.3–1.3 ± 0.3 n.u., P < 0.05). Changes in PWR correlated inversely with sympathetic and directly with vagal modulation in CE. In conclusion, AE, RE, and CE lowered central aortic pressure, but only AE and CE reduced PWR. Overall, those reductions related to decreased parasympathetic and increased sympathetic outflows. Autonomic fluctuations seemed to represent more a consequence than a cause of reduced PWR.
Gut microbiota changes related to Helicobacter pylori eradication with vonoprazan containing triple therapy among adolescents: a prospective multicenter study
Authors: Kakiuchi Toshihiko , Yamamoto Kentaroh , Imamura Ichiro , Hashiguchi Kazutoshi , Kawakubo Hiroharu , Yamaguchi Daisuke , Fujioka Yasuhiko , Okuda Masumi
Published: January 2021

Currently, it is unclear whether treating Helicobacter pylori (H. pylori) infection is safe among adolescents. This study aimed to evaluate the safety of H. pylori eradication therapy by examining gut microbiota changes in adolescents 3 months after the therapy. H. pylori-infected adolescents were enrolled in this study. Their stool samples were collected at the following three time points: before treatment, 1–2 days after completion of treatment, and time of eradication successful judgment. We assessed the relative abundance, alpha-diversity, and beta-diversity of the gut microbiota and adverse events. The number of isolated Actinobacteria decreased immediately after eradication therapy in the 16 students included in the study, and it returned to pretreatment condition at the eradication judgment point. There was no change in the relative abundance at genus level. The alpha-diversity was lost immediately after eradication therapy; however, it recovered at the time of eradication judgment, and it was restored to pretreatment condition. Meanwhile, none of the participants experienced serious adverse events. H. pylori eradication therapy is safe for adolescents with respect to gut microbiota changes associated with H. pylori eradication therapy. Therefore, further long-term evaluations of gut microbiota changes following eradication therapy are warranted.
Comparing the protective effects of resveratrol, curcumin and sulforaphane against LPS/IFN-γ-mediated inflammation in doxorubicin-treated macrophages
Authors: Saleh Haidy A. , Ramdan Eman , Elmazar Mohey M. , Azzazy Hassan M. E. , Abdelnaser Anwar
Published: January 2021

Doxorubicin (DOX) chemotherapy is associated with the release of inflammatory cytokines from macrophages. This has been suggested to be, in part, due to DOX-mediated leakage of endotoxins from gut microflora, which activate Toll-like receptor 4 (TLR4) signaling in macrophages, causing severe inflammation. However, the direct function of DOX on macrophages is still unknown. In the present study, we tested the hypothesis that DOX alone is incapable of stimulating inflammatory response in macrophages. Then, we compared the anti-inflammatory effects of curcumin (CUR), resveratrol (RES) and sulforaphane (SFN) against lipopolysaccharide/interferon-gamma (LPS/IFN-γ)-mediated inflammation in the absence or presence of DOX. For this purpose, RAW 264.7 cells were stimulated with LPS/IFN-γ (10 ng/mL/10 U/mL) in the absence or presence of DOX (0.1 µM). Our results showed that DOX alone is incapable of stimulating an inflammatory response in RAW 264.7 macrophages. Furthermore, after 24 h of incubation with LPS/IFN-γ, a significant increase in tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and inducible nitric oxide synthase (iNOS) mRNA levels was observed. Similarly, nitric oxide (NO) production and TNF-α and IL-6 protein levels were significantly upregulated. Moreover, in LPS/IFN-γ-treated macrophages, the microRNAs (miRNAs) miR-146a, miR-155, and miR-21 were significantly overexpressed. Interestingly, upon testing CUR, RES, and SFN against LPS/IFN-γ-mediated inflammation, only SFN was able to significantly reverse the LPS/IFN-γ-mediated induction of iNOS, TNF-α and IL-6 and attenuate miR-146a and miR-155 levels. In conclusion, SFN, at the transcriptional and posttranscriptional levels, exhibits potent immunomodulatory action against LPS/IFN-γ-stimulated macrophages, which may indicate SFN as a potential treatment for DOX-associated inflammation.
The molecular mechanisms of the long noncoding RNA SBF2-AS1 in regulating the proliferation of oesophageal squamous cell carcinoma
Authors: Zha Wenjuan , Li Xiaomin , Tie Xiaowei , Xing Yao , Li Hao , Gao Fei , Ye Ting , Du Wangqi , Chen Rui , Liu Yangchen
Published: January 2021

The long noncoding RNASBF2-AS1 can promote the occurrence and development of many kinds of tumours, but its role in oesophageal squamous cell carcinoma (ESCC) is unknown. We found that SBF2-AS1 was up-regulated in ESCC, and its expression was positively correlated with tumor size (P = 0.0001), but was not related to gender, age, TNM stage, histological grade, and lymphnode metastasis (P > 0.05). It was further found that the higher the expression of SBF2-AS1, the lower the survival rate. COX multivariate analysis showed that the expression of SBF2-AS1 was an independent prognostic factor. Functional experiments show that inhibition of SBF2-AS1 can inhibit the proliferation of ESCC through in vivo and in vitro, and overexpression of SBF2-AS1 can promote the proliferation of ESCC and inhibit its apoptosis. In mechanism, SBF2-AS1/miR-338-3P, miR-362-3P/E2F1 axis are involved in the regulation of ESCC growth. In general, SBF2-AS1 may be used as ceRNA to combine with miR-338-3P and miR-362-3P to up-regulate the expression ofE2F1, and ultimately play a role in promoting cancer. It may be used as a therapeutic target and a biomarker for prognosis.
Knowledge and awareness about human papillomavirus infection and its vaccination among women in Arab communities
Authors: Alsous Mervat M. , Ali Ahlam A. , Al-Azzam Sayer I. , Abdel Jalil Mariam H. , Al-Obaidi Hala J. , Al-abbadi Esraa I. , Hussain Zainab K. , Jirjees Feras J.
Published: January 2021

Cervical cancer (CC) is one of the most common types of cancer that affect females worldwide with hundreds of thousands of women dying annually due to this disease, mainly in developing countries. Infection with human papillomavirus (HPV) is the main risk factor for this cancer. There are no public awareness and national immunization programs in most Arab countries. This study aimed to investigate the knowledge and awareness about the HPV vaccine among females in four Arab countries and their acceptance to receive the vaccine. A cross-sectional study was conducted in several Arab countries: Jordan, Qatar, the United Arab Emirates (UAE), and Iraq. Respondents that fulfilled the desired criteria and were willing to participate in the study were asked to fill out the survey. Knowledge and awareness were assessed using 13 questions. Ethical approvals were given from the four countries. A total of 3658 individuals participated in the study; however, 2804 responses were included in the analysis and more than one third of participants (n = 1007) were aged between 18 and 25 years old. This study revealed poor awareness and knowledge of the participants about HPV and its vaccine among all four countries’ participants with relatively better knowledge among participants from the UAE. Participants who are younger (18–25 years old), have a postgraduate education, have an education or career related to the medical field, or had a Pap smear in the last three years tend to have higher knowledge about the HPV vaccine compared to others. Poor knowledge and awareness findings in this study were expected, considering the lack of public education campaigns regarding the HPV virus coupled with the absence of the HPV vaccination from the national immunization schedule in three participating countries (Jordan, Qatar, and Iraq). It is recommended that there is a need to provide national educational campaigns about the HPV vaccine to the public in all Arab populations.
Combination therapy of cisplatin with cilastatin enables an increased dose of cisplatin, enhancing its antitumor effect by suppression of nephrotoxicity
Authors: Arita Masashi , Watanabe Satoshi , Aoki Nobumasa , Kuwahara Shoji , Suzuki Ryo , Goto Sawako , Abe Yuko , Takahashi Miho , Sato Miyuki , Hokari Satoshi , Ohtsubo Aya , Shoji Satoshi , Nozaki Koichiro , Ichikawa Kosuke , Kondo Rie , Hayashi Masachika , Ohshima Yasuyoshi , Kabasawa Hideyuki , Hosojima Michihiro , Koya Toshiyuki , Saito Akihiko , Kikuchi Toshiaki
Published: January 2021

Cisplatin, one of the most active anticancer agents, is widely used in standard chemotherapy for various cancers. Cisplatin is more poorly tolerated than other chemotherapeutic drugs, and the main dose-limiting toxicity of cisplatin is its nephrotoxicity, which is dose-dependent. Although less toxic methods of cisplatin administration have been established, cisplatin-induced nephrotoxicity remains an unsolved problem. Megalin is an endocytic receptor expressed at the apical membrane of proximal tubules. We previously demonstrated that nephrotoxic drugs, including cisplatin, are reabsorbed through megalin and cause proximal tubular cell injury. We further found that cilastatin blocked the binding of cisplatin to megalin in vitro. In this study, we investigated whether cilastatin could reduce cisplatin-induced nephrotoxicity without influencing the antitumor effects of cisplatin. Nephrotoxicity was decreased or absent in mice treated with cisplatin and cilastatin, as determined by kidney injury molecule-1 staining and the blood urea nitrogen content. Combined with cilastatin, a twofold dose of cisplatin was used to successfully treat the mice, which enhanced the antitumor effects of cisplatin but reduced its nephrotoxicity. These findings suggest that we can increase the dose of cisplatin when combined with cilastatin and improve the outcome of cancer patients.
Validation of efficacy and mechanism of Sanwei-Tanxiang powder in improving myocardial ischemia reperfusion injuries
Authors: Sun Yu-Hui , Bu Ren , Wang Yue-Wu , Hu Yu-Chong , Wang Xu-Mei , Dong Xin , Zu Wen , Niu Yan , Zhao Peng-Wei , Sun Peng , Ru Shi-Hang , Lu Jing-Kun , Na Sheng-Sang
Published: January 2021

Sanwei-Tanxiang powder (SWTX), a traditional Mongolian and Tibetan medicine containing a cocktail of active molecules, relieves angina pectoris and improves recovery in patients with coronary heart disease (CHD). The pharmacological effect of SWTX on CHD was analyzed at a systemic point of view in our previous studies. The bioinformatics prediction showed that the PI3K/Akt/FoxO3a pathway was one of important pathways of SWTX on treatment of coronary heart disease. Based on it, the aim of this study was to evaluate the benefits of SWTX in acute myocardial ischemic-reperfused (MIR) rat in vivo and H9c2 cardiomyoblast cells under oxidative stress induced by H2O2 in vitro, and further investigate the involvement of PI3K/Akt/FoxO3a pathway in these processes. Ex vivo, under physiological conditions, SWTX did not show any modification in the heart rate and contraction amplitude. However, against a MIR injury, SWTX pretreatment provided significant protection, including reduced ST-segment elevation, pathological changes and myocardial infarct size in vivo, meanwhile, some monomers of SWTX showed antioxidant capacity and inhibited cardiomyocytic apoptosis in vitro. The effect was correlated with the activation of the PI3K/Akt/FoxO3a signaling pathway downstream and the regulation of downstream pro-apoptotic Bim of FoxO3a experimental verified by qRT-PCR, Western blot and immunofluorescent assay. In vitro, blocking Akt and p-FoxO3a activation with the PI3K inhibitor LY294002 effectively suppressed the protective effects of several active monomers (including quercetin, macelignan,methyleugenol and Santol) of SWTX against H2O2-induced injury. Collectively, these results suggest that SWTX decreases I/R injury, and the PI3K/Akt/FoxO3a pathway takes part in protection during this process, gallogen (G3) and quercetin (G8) of GZ, methyleugenol (R2) and macelignan (R7) of RDK, santol (T1) of TX are responsible at least in part for SWTX’s cardioprotection effect.
Cerebrospinal fluid metabolomics identifies 19 brain-related phenotype associations
Authors: Panyard Daniel J. , Kim Kyeong Mo , Darst Burcu F. , Deming Yuetiva K. , Zhong Xiaoyuan , Wu Yuchang , Kang Hyunseung , Carlsson Cynthia M. , Johnson Sterling C. , Asthana Sanjay , Engelman Corinne D. , Lu Qiongshi
Published: January 2021

The study of metabolomics and disease has enabled the discovery of new risk factors, diagnostic markers, and drug targets. For neurological and psychiatric phenotypes, the cerebrospinal fluid (CSF) is of particular importance. However, the CSF metabolome is difficult to study on a large scale due to the relative complexity of the procedure needed to collect the fluid. Here, we present a metabolome-wide association study (MWAS), which uses genetic and metabolomic data to impute metabolites into large samples with genome-wide association summary statistics. We conduct a metabolome-wide, genome-wide association analysis with 338 CSF metabolites, identifying 16 genotype-metabolite associations (metabolite quantitative trait loci, or mQTLs). We then build prediction models for all available CSF metabolites and test for associations with 27 neurological and psychiatric phenotypes, identifying 19 significant CSF metabolite-phenotype associations. Our results demonstrate the feasibility of MWAS to study omic data in scarce sample types.
Extended life cycle assessment reveals the spatially-explicit water scarcity footprint of a lithium-ion battery storage
Authors: Schomberg Anna C. , Bringezu Stefan , Flörke Martina
Published: January 2021

The life cycle water scarcity footprint is a tool to evaluate anthropogenic contributions to regional water scarcity along global supply chains. Here, we complement it by a classification of the risk from human water use, a comprehensive conceptualisation of water use and a spatially-explicit impact assessment to a midpoint approach that assesses the risk of on-site and remote freshwater scarcity. For a 2 MWh Lithium-ion battery storage, the quantitative Water Scarcity Footprint, comprising physically used water, accounts for 33,155 regionally weighted m3 with highest contributions from Chilean lithium mining. The qualitative Water Scarcity Footprint, the virtual volume required to dilute pollutant emissions to safe concentrations, is approximately determined to 52 million m3 of regionally weighted demineralised water with highest contributions from copper and aluminium mining operations. As mining operations seem to have the highest impact, we recommend to consider the spatially-explicit water scarcity footprint for assessment of global material supply.
Acridinium benzoates for ratiometric fluorescence imaging of cellular viscosity
Authors: Wen Min , Li Chen , Zhang Mengyuan , Sun Yan , Liu Fengming , Cui Xiaoyan , Shan Yongkui
Published: January 2021

A series of fluorescent molecular rotors, acridinium benzoates, were designed for ratiometrically monitoring cellular viscosity with high sensitivity.
Supramolecular spectral/visual detection of urinary polyamines through synergetic/competitive complexation with γ-CD and CB[7]
Authors: Tian Haoyu , Yu Xingke , Yao Jiabin , Gao Guowei , Wu Wanhua , Yang Cheng
Published: January 2021

A supramolecular strategy for detecting the concentration of polyamines has been established through competitive/synergetic complexation among polyamines, CB[7], γ-CD, and pyrene derivatives, which allows for convenient, rapid, and high throughput spectral/visual detection of the concentration of urinary polyamines based on the switching on/off of the pyrene excimer fluorescence.
Performances variations of BiFeO3-based ceramics induced by additives with diverse phase structures
Authors: Shi Yunjing , Yan Fei , He Xia , Zhu Kun , Li Guohui , Dong Xiaoyu , Shen Bo , Zhai Jiwei
Published: January 2021

Obviously distortion occurred in the BFBT ceramics after four different space groups of ABO3 perovskite doping. The changed electronic structures resulted in variety polarization, leading to distinction electrical performances of ceramics.
Acid rain formation through catalytic transformation of sulfur dioxide over clay dusts: remarkable promotion by a vicinal aluminium site
Authors: Yang Gang , Zhou Lijun
Published: January 2021

Mechanisms for catalytic SO2transformation to H2SO4over clay dusts have been unraveled at a molecular level. All O atoms in ozone (especially molecular oxygen) are effective oxidants due to remarkable promotion of a vicinal Al3+site.
Luminescent silver(i) complexes with pyrazole-tetraphenylethene ligands: turn-on fluorescence due to the coordination-driven rigidification and solvent-oriented structural transformation
Authors: Xu Zou-Hong , Huang Zi-Qing , Liu Xiao-Hui , Zhao Yue , Lu Yi , Sun Wei-Yin
Published: January 2021

A series of Ag complexes 1–7 with pyrazole-tetraphenylethene ligands were fabricated and their fluorescence and sensing properties were examined.
Theoretical investigation of tetrahedral distortion of four-coordinate iron(ii) centres in FePd(CN)4
Authors: Fujii Susumu , Ohtani Ryo , Kuwabara Akihide
Published: January 2021

The Jahn–Teller-like effect on the tetrahedral geometry of d6 iron(ii) is caused by its unequally occupied e orbitals with non-bonding nature.
Mechanical, thermal, and microstructural analyses of thermoplastic poly(2-methoxyethyl acrylate)-based polyurethane by RAFT and polyaddition
Authors: Tazawa Shunsuke , Maeda Tomoki , Hotta Atsushi
Published: January 2021

A thermoplastic solid poly(2-methoxyethyl acrylate)(PMEA)-based polyurethane(PU) with excellent thermo-mechanical properties and antithrombogenicity was obtained by increasing the molecular weight of PMEA-based PU to ∼80 k.
Correction: Single nanosheet can sustainably generate oxygen and inhibit respiration simultaneously in cancer cells
Authors: Huang Wei-Qiang , Wang Fei , Shen Ai-Zong , Zhang Lei , Nie Xuan , Zhang Ze , Chen Guang , Xia Lei , Wang Long-Hai , Ding Sheng-Gang , Meng Qing-Yong , Zhang Wen-Jian , Hong Chun-Yan , You Ye-Zi
Published: January 2021

Correction for ‘Single nanosheet can sustainably generate oxygen and inhibit respiration simultaneously in cancer cells’ by Wei-Qiang Huang et al., Mater. Horiz., 2021, DOI: 10.1039/d0mh01446j.
Tuning the band gap of M-doped titanate nanotubes (M = Fe, Co, Ni, and Cu): an experimental and theoretical study
Authors: Méndez-Galván Melissa , Celaya Christian A. , Jaramillo-Quintero Oscar Andrés , Muñiz Jesus , Díaz Gabriela , Lara-García Hugo A.
Published: January 2021

The Ti-NT bandgap can be tuned in a wide range (1.5–3.3 eV) by an easy ion-exchange method using a low concentration (1 wt%) of Cu2+, Ni2+, Co2+, and Fe3+ cations.
Preparation of Cu cluster catalysts by simultaneous cooling–microwave heating: application in radical cascade annulation
Authors: Song Liangliang , Manno Roberta , Ranjan Prabhat , Sebastian Victor , Irusta Silvia , Mallada Reyes , Van Meervelt Luc , Santamaria Jesús , Van der Eycken Erik V.
Published: January 2021

The sub-1.5 nm Cu clusters are prepared through simultaneous cooling–microwave heating and employed for the synthesis of various polycycles.