bluuebunny/crossref_metadata_2025 huggingface dataset:
The Quantification and Tracing of Leakage in the Forest Sector in Nordic Countries
Authors: Hu Junhui , Jåstad Eirik Ogner , Bolkesjø Torjus Folsland , Rørstad Per Kristian
Published: January 2024

This study examines production leakage in four Nordic countries and its impact on industries and the global market. Using a Forest Sector Model, we analyze the effects of changing the harvest and find the leakage of roundwood harvest to range from 61% to 76% in Norway, 59% to 81% in Sweden, 57% to 89% in Finland, and 59% to 106% in Denmark. Notably, trade with other parts of the world absorbs over 50% of roundwood harvest changes in a Nordic country, with Norway exhibiting distinct trade patterns compared to Finland and Sweden. Compared to prior findings, sawnwood production leakage is smaller, underscoring the necessity for the refined modeling of non-Nordic countries. Importantly, our findings contribute insights into forest industries, leakage, and global trade dynamics, relevant to the Nordic context and having broader implications for globally interconnected countries.
The Possibility of Using Non-Native Spruces for Norway Spruce Wood Replacement—A Case Study from the Czech Republic
Authors: Zeidler Aleš , Borůvka Vlastimil , Brabec Pavel , Tomczak Karol , Bedřich Jakub , Vacek Zdeněk , Cukor Jan , Vacek Stanislav
Published: January 2024

European forests are facing ongoing climate change, and certain tree species are being critically impacted. The Norway spruce (Picea abies (L.) Karst.) is one of the most sensitive species to climate fluctuations, a fact manifesting itself through massive dieback resulting in a lack of high-quality timber and timber market destabilization. Therefore, the possibility of wood substitution with non-native spruce species, namely, black spruce (Picea mariana (Mill.) Britt., Sterns, et Poggenburg), Serbian spruce (Picea omorika (Pančić) Purk.), and blue spruce (Picea pungens Engelm.), under the specific conditions of forest reclamations with great potential for future afforestation was tested. Wood density, modulus of rupture, and modulus of elasticity were used to evaluate wood quality in comparison with native Norway spruce. The results confirmed that only the Serbian spruce reached the quality of Norway spruce and even exceeded it in terms of wood density (P. omorika 525 kg·m−3 vs. P. abies 517 kg·m−3) and exhibited comparable parameters with regard to other properties. The density of the other species was significantly lower for blue spruce (476 kg·m−3) and black spruce (468 kg·m−3). A similar trend was found for other wood parameters, which confirmed that Norway spruce quality was nearly comparable with that of Serbian spruce. On the other hand, black spruce and blue spruce did not match the quality of Norway spruce. The within-stem variability of the properties tested was low for all the spruce species examined. In conclusion, the Serbian spruce showed great potential for future usage in forest management and is one of the possible methods of Norway spruce replacement in times of unprecedented forest disturbances under the effects of global climate change.
The Dynamics and Potential of Carbon Stocks as an Indicator of Sustainable Development for Forest Bioeconomy in Ghana
Authors: Nyarko Isaac , Nwaogu Chukwudi , Diagi Bridget E. , Hájek Miroslav
Published: January 2024

Sustainable forest bioeconomy (SFB), as a multidimensional approach for establishing mutual benefits between forest ecosystems, the environment, the economy, and humans, is a nature-based solution for a promising future. The study aims to evaluate the potential of carbon stocks (Cstocks) and variability for SFB. It is hypothesized that the decrease in Cstocks is related to an increase in population and agriculture, which caused a decrease in forest area and growing stock and consequently affected SFB. Primary and secondary data were collected from the field, national, and international databases, and analyzed using some statistical and geospatial software packages including IBM SPSS 29.0, CANOCO 5.0, and ArcGIS 10.5. The results revealed that large forest areas were converted to arable lands between 2000 and 2020. Across the forest zones, the aboveground and belowground Cstocks varied significantly, with the aboveground biomass being higher than the belowground biomass. The main drivers of Cstocks were politics and governance (57%), population growth (50%), soil degradation practices (50%), and socio-cultural beliefs (45%). Cstocks had significant negative correlation with population growth, carbon emissions, forest growing stock, forest loss, and the use of forest for biofuel. Evergreen forest zones (rainforest and moist) had more Cstocks than the moist deciduous and swamp/mangrove forests. The study demonstrated that the variability in Cstocks over the last three decades is attributed to an increase in population and agriculture, but Cstocks variability between the forest-vegetation belts could be better explained by differences in trees abundance than population. The study also revealed that the increase in Cstocks contributed to the realization of many SDGs, especially SDG 1, 2, 3, 6, 7, 11, 12, 13, and 15, which in turn support a sustainable forest bioeconomy. Future study is necessary to evaluate Cstocks in individual tree species, biodiversity, and other forest ecosystem services to promote SFB in the country.
The Phytoremediation Potential of 14 Salix Clones Grown in Pb/Zn and Cu Mine Tailings
Authors: Jiang Chenggang , Wang Yangdong , Chen Yitai , Wang Shufeng , Mu Changcheng , Shi Xiang
Published: January 2024

Willows are suitable candidates for phytoremediation projects. A pot experiment was conducted to evaluate the potential of using Salix unrooted cuttings for the phytoremediation of lead/zinc (Pb/Zn) and copper (Cu) mine tailings. Cuttings of 14 Salix clones were directly rooted into pots containing mine tailings. The 14 clones showed different levels of tolerance to tailing treatments. A total of 71.40% and 85.70% of the S. jiangsuensis ‘172’ cuttings either grown in Pb/Zn or Cu tailings survived, respectively. However, the other clones had lower survival rates, and the values were no more than 40%. Usually, all clones produce less biomass in an extremely contaminated environment. Clonal variation in biomass yield was observed in this research. The surviving clones, such as S. integra ‘WSH’, S. matsudana ‘14’, S. chaenomeloides ‘3’, S. chaenomeloides ‘4’, and S. chaenomeloides ‘5’ (Pb/Zn tailing), S. integra ‘HY’, S. integra ‘WSH’, S. matsudana ‘14’, S. matsudana ‘19’, and S. matsudana ‘34’ (Cu tailing) produced relatively more biomass in this study. In general, all the clones presented lower bioconcentration factor values for the tailings of heavy metals. In principle, all clones could easily take up and translocate Zn and Cd from the tailings to aboveground parts, especially S. integra. All clones exhibited a huge variation in their heavy metal accumulation capacity. As stated above, the direct utilization of cuttings for phytoremediation is a viable option. S. jiangsuensis ‘172’ had a high tolerance capacity and would be a recommended candidate for future phytoremediation projects in soils containing tailings with an extremely high concentration of heavy metals. These results provide crucial information about willow growth and metal accumulation capacity in extremely adverse environments.
Effects of Stand Types on Ectomycorrhizal Fungal Community Composition and Structure of Pinus massoniana in Subtropical Mountain Forest Ecosystems
Authors: Jiang Dandan , Xu Ming , Yang Yunli , Zhang Jian
Published: January 2024

Tree species composition in forest ecosystems is an important biological factor affecting the diversity of ectomycorrhizal fungi (EMF). However, little is known about the composition and diversity of EMF communities associated with Pinus massoniana in different P. massoniana association habitats (MpAHs) in subtropical mountains. This study investigated the EMF community characteristics of P. massoniana in different MpAHs using plant community surveys, soil property analyses, and mycorrhizal identification. A total of 56 operational taxonomic units (OTUs), belonging to 20 families and 22 genera, were identified. OTU richness of Basidiomycota (58.93%) was higher than that of Ascomycota (41.07%). Unclassified Helotiales, Russula, Lactarius, and Tomentella were the dominant groups. Different stand types significantly altered the EMF communities of P. massoniana (p < 0.05, for Shannon index) and the associations of P. massoniana + Populus adenopoda (Mp_Pa) had the highest diversity of EMF, while P. massoniana + Cunninghamia lanceolata (Mp_Cl) had the lowest diversity. The number of specific OTUs was higher than shared OTUs. Similarity index and principal coordinate analysis indicated that the EMF communities of P. massoniana varied significantly in different MpAHs (R2 = 0.21, p = 0.001). The linear regression model showed that the EMF diversity of P. massoniana was positively related to tree species diversity, indicating that the EMF diversity of P. massoniana is influenced by tree species diversity. The findings provide a reasonable reference for tree species configuration in the process of mixed transformation or near-natural management of plantations.
Applicability of Thermal Dissipation Method in Sap Flow Measurement of Karst Shrub Community
Authors: Qin Jiashuang , Ni Longkang , Gu Daxing , Yang Kaijun , Huang Qibo , Tan Yu , Liao Suhui
Published: January 2024

Although the thermal dissipation probe (TDP) method is prominent in forest transpiration studies, the accurate estimation of sap flow density in particular trees with different wood structures remains highly variable. To verify the applicability of the TDP method in the measurement of the transpirational water consumption of shrub communities in degraded karst areas in southern China, Granier’s original formula was validated and corrected using the isolated stem segment method with Celtis sinensis, Xylosma congesta, Triadica sebifera, Paliurus ramosissimus, Pyracantha fortuneana, Sageretia thea, and Phanera championii. Finally, the correction coefficients were analyzed in relation to wood structural parameters. Our study reveals that the sap flow density estimated using Granier’s original formula exhibited varying degrees of overestimation or underestimation compared to the measured sap flow density. The differences ranged from −78.9% to 114.2%, highlighting the need for formula correction. Consequently, we adjusted the coefficients α and β of the original formula (n = 3–5), resulting in a revised range of 0.0047–0.0509 g cm−2 s−1 and 0.5882–1.9821 (R2 = 0.81–0.97), respectively. The conduit diameter was significantly correlated with the correction coefficient α. It is currently unknown whether the results obtained for a particular species can be applied to different growth environments and more prominent individuals of the same species. In conclusion, it is necessary to verify the applicability of Granier’s original formula when estimating water consumption through transpiration in trees.
The Relationship between the Carbon Fixation Capacity of Vegetation and Cultivated Land Expansion and Its Driving Factors in an Oasis in the Arid Region of Xinjiang, China
Authors: Sun Mengting , Jiang Hongnan , Xu Jianhui , Zhou Peng , Li Xu , Xie Mengyu , Hao Doudou
Published: January 2024

In the process of agricultural development in arid and semi-arid areas, the carbon fixation capacity of vegetation can be affected to different degrees, but research on its driving factors is lacking. Consequently, this paper focuses on the Weiku Oasis in Xinjiang as its research area, in which the carbon fixation capacity of vegetation is estimated with the chemical equation of a photochemical reaction, using methods such as linear system models and Geodetector to analyze the relationship between cropland expansion characteristics and the carbon fixation capacity of vegetation from 1990 to 2020. The influence of land-use changes on the space differentiation of carbon fixation was elucidated through a time series relationship, and the synergistic effects of nine influencing factors on the carbon fixation capacity during the process of vegetation changes were discussed. The results were as follows: (1) In the process of agricultural development, the proportions of cultivated land area and spatial agglomeration had significant negative correlations with carbon sequestration, and the significance was rising, but the effect of cultivated land area proportion was more significant. (2) Through temporal sequential cooperativity analysis, when other land-use types were converted into cultivated land, the carbon fixation capacity of vegetation suddenly and significantly decreased in the initial year of the transformation, but the effect of cultivated land reclamation on the carbon fixation capacity of vegetation did not have a significant time lag. Moreover, after a certain period of time, cultivated land can gradually recover part of its lost carbon fixation capacity. (3) Among the nine driving factors, potential evapotranspiration is the most prominent in explaining the carbon fixation capacity of vegetation. This single-factor pairwise interaction presents the relationship between bivariate enhancement and nonlinear enhancement. When terrain factors interact with other factors, the enhancement effect of the influence on the carbon fixation capacity of vegetation has an obvious promotion effect. However, the change in the carbon fixation capacity of vegetation is more significantly influenced by potential evapotranspiration and the interaction between the normalized difference vegetation index (NDVI) and other factors. This research is helpful to understanding the basic theories related to the change in the carbon fixation capacity of vegetation during the process of agricultural development in arid and semi-arid areas, as well as providing theoretical reference for ecological environment construction and sustainable development.
Autoplant—Autonomous Site Preparation and Tree Planting for a Sustainable Bioeconomy
Authors: Hansson Linnea J. , Sten Gustav , Rossander Morgan , Lideskog Håkan , Manner Jussi , van Westendorp Ruben , Li Songyu , Eriksson Anders , Wallner Anna , Rönnqvist Mikael , Flisberg Patrik , Edlund Björn , Möller Björn , Karlberg Magnus
Published: January 2024

Sustainable forestry requires efficient regeneration methods to ensure that new forests are established quickly. In Sweden, 99% of the planting is manual, but finding labor for this arduous work is difficult. An autonomous scarifying and planting machine with high precision, low environmental impact, and a good work environment would meet the needs of the forest industry. For two years, a collaborative group of researchers, manufacturers, and users (forest companies) has worked together on developing and testing a new concept for autonomous forest regeneration (Autoplant). The concept comprises several subsystems, i.e., regeneration and route planning, autonomous driving (path planning), new technology for forest regeneration with minimal environmental impact, automatic plant management, crane motion planning, detection of planting spots, and follow-up. The subsystems were tested separately and integrated together during a field test at a clearcut. The concept shows great potential, especially from an environmental perspective, with significantly reduced soil disturbances, from approximately 50% (the area proportion of the area disturbed by disc trenching) to less than 3%. The Autoplant project highlights the challenges and opportunities related to future development, e.g., the relation between machine cost and operating speed, sensor robustness in response to vibrations and weather, and precision in detecting the size and type of obstacles during autonomous driving and planting.
Social Inequity and Wildfire Response: Identifying Gaps and Interventions in Ventura County, California
Authors: Baker Brianna , Dinh Yvonne , Foxfoot Iris R. , Ortiz Elena , Sells Alison , Anderson Sarah E.
Published: January 2024

As climate change increases the frequency and severity of wildfires across the Western U.S., there is an urgent need for improved wildfire preparedness and responses. Socially marginalized communities are particularly vulnerable to wildfire effects because they disproportionately lack access to the resources necessary to prepare for and recover from wildfire and are frequently underrepresented in the wildfire planning process. As an exemplar of how to understand and improve preparedness in such communities, this research identified communities in Ventura County facing heightened marginalization and risk of wildfire using spatial analysis. Researchers then deployed a county-wide survey and held focus groups in two communities identified in the spatial analysis. Research revealed that non-English speakers, women, people of color, and newer residents in Ventura County are less prepared for wildfire than other groups. Based on these findings, this paper recommends an expansion of traditional risk mitigation programs, strengthened community engagement efforts, and strategies that increase community resources and leadership to decouple marginalization and wildfire vulnerability.
Activation Energy of Hydrogen–Methane Mixtures
Authors: Moroshkina Anastasia , Ponomareva Alina , Mislavskii Vladimir , Sereshchenko Evgeniy , Gubernov Vladimir , Bykov Viatcheslav , Minaev Sergey
Published: January 2024

In this work, the overall activation energy of the combustion of lean hydrogen–methane–air mixtures (equivalence ratio φ = 0.7−1.0 and hydrogen fraction in methane α=0, 2, 4) is experimentally determined using thin-filament pyrometry of flames stabilised on a flat porous burner under normal conditions (p=1 bar, T = 20 °C). The experimental data are compared with numerical calculations within the detailed reaction mechanism GRI3.0 and both approaches confirm the linear correlation between mass flow rate and inverse flame temperature predicted in the theory. An analysis of the numerical and experimental data shows that, in the limit of lean hydrogen–methane–air mixtures, the activation energy approaches a constant value, which is not sensitive to the addition of hydrogen to methane. The mass flow rate for a freely propagating flame and, thus, the laminar burning velocity, are measured for mixtures with different hydrogen contents. This mass flow rate, scaled over the characteristic temperature dependence of the laminar burning velocity for a one-step reaction mechanism, is found and it can also be used in order to estimate the parameters of the overall reaction mechanisms. Such reaction mechanisms will find implementation in the numerical simulation of practical combustion devices with complex flows and geometries.
The Public Health Importance and Management of Infectious Poultry Diseases in Smallholder Systems in Africa
Authors: Grace Delia , Knight-Jones Theodore J. D. , Melaku Achenef , Alders Robyn , Jemberu Wudu T.
Published: January 2024

Poultry diseases pose major constraints on smallholder production in Africa, causing high flock mortality and economic hardship. Infectious diseases, especially viral diseases like Newcastle disease and highly pathogenic avian influenza (HPAI) and bacterial diseases, especially colibacillosis and salmonellosis, are responsible for most chicken losses, with downstream effects on human nutrition and health. Beyond production impacts, poultry diseases directly harm public health if zoonotic, can give rise to epidemics and pandemics, and facilitate antimicrobial resistance through treatment attempts. HPAI, campylobacteriosis, and salmonellosis are the priority zoonoses. Sustainable solutions for poultry health remain elusive despite recognition of the problem. This review summarises current knowledge on major poultry diseases in smallholder systems, their impacts, and options for prevention and control. We find biosecurity, vaccination, good husbandry, and disease-resistant breeds can reduce disease burden, but practical limitations exist in implementing these measures across smallholder systems. Treatment is often inefficient for viral diseases, and treatment for bacterial diseases risks antimicrobial resistance. Ethnoveterinary practices offer accessible alternatives but require more rigorous evaluation. Multisectoral collaboration and policies that reach smallholder poultry keepers are essential to alleviate disease constraints. Successful control will improve livelihoods, nutrition, and gender equity for millions of rural families. This review concludes that sustainable, scalable solutions for smallholder poultry disease control remain a critical unmet need in Africa.
Assessing Matrix Solid-Phase Dispersion Extraction Strategies for Determining Bisphenols and Phthalates in Gilthead Sea Bream Samples
Authors: Soliz Dulce L. , Garcinuño Rosa Ma , Paniagua González Gema , Bravo Juan Carlos , Fernández Hernando Pilar
Published: January 2024

Microplastics (MPs) and nanoplastics (NPs) are widely spread in the environment, generating significant concern due to their potential impact on environmental health. Marine species usually ingest plastic fragments, mistaking them for food. Many toxic compounds, such as plastic additives that are not chemically bound to the plastic matrix, can be released from MPs and NPs and reach humans via the food chain. This paper highlights the development and validation of a straightforward solid–liquid extraction clean-up procedure in combination with a matrix solid-phase dispersion method using high-performance liquid chromatography coupled with mass spectrometry (HPLC-MS) detection, enabling facile, precise, and reliable identification and quantitation of a total of six bisphenols and phthalates in gilthead sea breams. Under the optimized conditions, the developed method showed good linearity (R2 > 0.993) for all target compounds. The recoveries obtained were between 70 and 92%. The relative standard deviations (RSDs) for reproducibility (inter-day) and repeatability (intra-day) were less than 9% and 10%, respectively. The limit of detection (LOD) and limit of quantification (LOQ) for the target compounds ranged from 0.11 to 0.68 µg/kg and from 0.37 to 2.28 µg/kg, respectively. A new, efficient extraction methodology for the determination of BPA, BPS, BPF, DBP, DEP, and DHEP in gilthead seabream has been optimized and validated.
Headspace Solid-Phase Microextraction/Gas Chromatography–Mass Spectrometry and Chemometric Approach for the Study of Volatile Profile in X-ray Irradiated Surface-Ripened Cheeses
Authors: Palermo Carmen , Mentana Annalisa , Tomaiuolo Michele , Campaniello Maria , Iammarino Marco , Centonze Diego , Zianni Rosalia
Published: January 2024

X-ray irradiation is an emerging non-thermal technology that is used as a preservation and sanitization technique to inactivate pathogens and spoilage organisms, increasing the shelf life of products. In this work, two different types of surface-ripened cheeses, Brie and Camembert, produced with cow milk, were treated with X-rays at three dose levels, 2.0, 4.0 and 6.0 kGy, to evaluate the irradiation effects on the volatile profile using a volatolomic approach. The headspace solid-phase microextraction (HS-SPME) technique combined with gas chromatography–mass spectrometry (GC–MS) was used to extract and analyze the volatile fraction from these dairy matrices. The HS-SPME method was optimized by a central composite design in combination with a desirability optimization methodology. The Carboxen/PDMS fiber, 50 °C for extraction temperature and 60 min for time extraction were found to be the best parameter settings and were applied for this investigation. The obtained fingerprints demonstrated that the irradiation-induced changes are dose dependent. The X-ray irradiation produced many new volatiles not found in the non-irradiated samples, but it also varied the amount of some volatiles already present in the control. Specifically, aldehydes and hydrocarbons increased with the irradiation dose, whereas alcohols, carboxylic acids, esters, methyl esters, ketones, lactones and sulfur-containing compounds showed a non-linear dependence on the dose levels; indeed, they increased up to 4.0 kGy, and then decreased slightly at 6.0 kGy. This trend, more evident in the Camembert profile, is probably due to the fact that these compounds are involved in different oxidation mechanisms of lipids and proteins, which were induced by the radiation treatment. In these oxidative chemical changes, the production and degradation processes of the volatiles are competitive, but at higher doses, the decomposition reactions exceed those of formation. A principal component analysis and partial least square discriminant analysis were used to discriminate between the treated and untreated samples. Moreover, this study allowed for the identification of potential markers of X-ray treatment for the two cheeses, confirming this approach as a useful tool for the control of irradiated surface-ripened cheeses.
Effect of Germination on the Physicochemical Properties, Functional Groups, Content of Bioactive Compounds, and Antioxidant Capacity of Different Varieties of Quinoa (Chenopodium quinoa Willd.) Grown in the High Andean Zone of Peru
Authors: Ramos-Pacheco Betsy S. , Choque-Quispe David , Ligarda-Samanez Carlos A. , Solano-Reynoso Aydeé M. , Palomino-Rincón Henry , Choque-Quispe Yudith , Peralta-Guevara Diego E. , Moscoso-Moscoso Elibet , Aiquipa-Pillaca Ángel S.
Published: January 2024

Germination is an effective strategy to improve the nutritional and functional quality of Andean grains such as quinoa (Chenopodium quinoa Willd.); it helps reduce anti-nutritional components and enhance the digestibility and sensory aspects of the germinated. This work aimed to evaluate the effect of germination (0, 24, 48, and 72 h) on the physicochemical properties, content of bioactive compounds, and antioxidant capacity of three varieties of quinoa: white, red, and black high Andean from Peru. Color, nutritional composition, mineral content, phenolic compounds, flavonoids, and antioxidant activity were analyzed. Additionally, infrared spectra were obtained to elucidate structural changes during germination. The results showed color variations and significant increases (p < 0.05) in proteins, fiber, minerals, phenolic compounds, flavonoids, and antioxidant capacity after 72 h of germination, attributed to the activation of enzymatic pathways. In contrast, the infrared spectra showed a decrease in the intensity of functional groups –CH–, –CH2–, C–OH, –OH, and C–N. Correlation analysis showed that flavonoids mainly contributed to antioxidant activity (r = 0.612). Germination represents a promising alternative to develop functional ingredients from germinated quinoa flour with improved nutritional and functional attributes.
In Vitro Antibacterial and Antioxidative Activity and Polyphenolic Profile of the Extracts of Chokeberry, Blackcurrant, and Rowan Berries and Their Pomaces
Authors: Meremäe Kadrin , Raudsepp Piret , Rusalepp Linda , Anton Dea , Bleive Uko , Roasto Mati
Published: January 2024

The chemical composition of berries and berry pomaces is diverse, containing polyphenolic components that may have both antibacterial and antioxidative properties. In the present study, in vitro antibacterial effect of the extracts of chokeberry, blackcurrant, and rowan berries and berry pomaces against L. monocytogenes, S. aureus, E. coli, and C. jejuni was studied. In addition, the polyphenolic profile and antioxidant activity of these extracts were investigated. The polyphenolic profiles in the aqueous and 30% ethanolic extracts were determined chromatographically by HPLC-MS, and the total polyphenol content was estimated spectrophotometrically by HPLC-DAD-UV. The minimal inhibition concentrations (MICs) of the extracts against tested bacteria were determined by the broth microdilution method. The content of total polyphenols was highest and good antioxidative properties of the extracts were determined for chokeberry and blackcurrant berries and their pomaces. The highest proportions of total quercetin derivatives and anthocyanins were found in the extracts of chokeberry berry/pomace and blackcurrant berry/pomace, respectively. The sensitivity of tested microbes to the extracts of berries and berry pomaces was as follows: S. aureus > L. monocytogenes > E. coli and C. jejuni. In vitro antibacterial activity of tested extracts depended on the extraction solvent, mainly for the ethanolic extracts. Findings suggest that chokeberry and blackcurrant berries and their pomaces can be used as a good source of polyphenols with antioxidative properties, and they also have antibacterial activity against some foodborne pathogenic bacteria. It is important that the valuable compounds are extracted from juice press residues before their disposal.
The Effect of Co-Culture with Different Pichia kluyveri and Saccharomyces cerevisiae on Volatile Compound and Characteristic Fingerprints of Mulberry Wine
Authors: Ding Bo , Zhao Shutian , Zhang Wenxue , Lin Ying , Xiong Ling
Published: January 2024

In this study, changes in volatile compounds co-fermented by different Pichia kluyveri with Saccharomyces cerevisiae were analyzed using GC-IMS and compared with S. cerevisiae fermentation, to investigate the production of aroma in mulberry wine during the fermentation process. A total of 61 compounds were accurately identified, including 21 esters, 10 alcohols, 8 aldehydes, 6 ketones, and 19 other volatiles. Compared with the single strain fermentation (S. cerevisiae), the content of 2-methylpropyl acetate, allyl Isothiocyanate, ethyl crotonate, isobutyl propanoate, and butyl 2-methylbutanoate, co-fermentation groups (S. cerevisiae with different P. kluyveri) showed a significant decrease. Alcohols, aldehydes, ketones, and organic acid were lower in both the F(S-P1) and F(S-P2) groups than in the F(S) group throughout fermentation. The 2-methylpentanoic acid only was contained in the F(S) group. The co-fermentation with different P. kluyveri could also be well distinguished. The content of Benzaldehyde and 4-methylphenol in the F(S-P1) group was significantly lower than that in the F(S-P2) group. The PCA results revealed effective differentiation of mulberry wine fermented by different fermentation strains from GC-IMS. The result showed that P. kluyveri could establish a new flavor system for mulberry wine, which plays a crucial role in enhancing the flavor of fruit wine.
Predictive Analysis of Linoleic Acid in Red Meat Employing Advanced Ensemble Models of Bayesian and CNN-Bi-LSTM Decision Layer Fusion Based Hyperspectral Imaging
Authors: Yan Xiuwei , Liu Sijia , Wang Songlei , Cui Jiarui , Wang Yongrui , Lv Yu , Li Hui , Feng Yingjie , Luo Ruiming , Zhang Zhifeng , Zhang Lei
Published: January 2024

Rapid non-destructive testing technologies are effectively used to analyze and evaluate the linoleic acid content while processing fresh meat products. In current study, hyperspectral imaging (HSI) technology was combined with deep learning optimization algorithm to model and analyze the linoleic acid content in 252 mixed red meat samples. A comparative study was conducted by experimenting mixed sample data preprocessing methods and feature wavelength extraction methods depending on the distribution of linoleic acid content. Initially, convolutional neural network Bi-directional long short-term memory (CNN-Bi-LSTM) model was constructed to reduce the loss of the fully connected layer extracted feature information and optimize the prediction effect. In addition, the prediction process of overfitting phenomenon in the CNN-Bi-LSTM model was also targeted. The Bayesian-CNN-Bi-LSTM (Bayes-CNN-Bi-LSTM) model was proposed to improve the linoleic acid prediction in red meat through iterative optimization of Gaussian process acceleration function. Results showed that best preprocessing effect was achieved by using the detrending algorithm, while 11 feature wavelengths extracted by variable combination population analysis (VCPA) method effectively contained characteristic group information of linoleic acid. The Bi-directional LSTM (Bi-LSTM) model combined with the feature extraction data set of VCPA method predicted 0.860 Rp2 value of linoleic acid content in red meat. The CNN-Bi-LSTM model achieved an Rp2 of 0.889, and the optimized Bayes-CNN-Bi-LSTM model was constructed to achieve the best prediction with an Rp2 of 0.909. This study provided a reference for the rapid synchronous detection of mixed sample indicators, and a theoretical basis for the development of hyperspectral on-line detection equipment.
Efficiency of Electronic Nose in Detecting the Microbial Spoilage of Fresh Sardines (Sardinella longiceps)
Authors: Al-Hooti Haitham S. , Al-Bulushi Ismail M. , Al-Attabi Zahir H. , Rahman Mohammad S. , Al-Subhi Lyutha K. , Al-Habsi Nasser A.
Published: January 2024

The assessment of microbial spoilage in fresh fish is a major concern for the fish industry. This study aimed to evaluate the efficiency and reliability of an electronic nose (E-nose) to detect microbial spoilage of fresh sardines (Sardinella longiceps) by comparing its measurements with Total Bacterial Count (TBC), Hydrogen Sulfide (H2S) producing bacterial count and Trimethylamine Oxide (TMAO) reducing bacterial count after variable storage conditions. The samples were stored at 0 °C (0, 2, 4, 6, and 8 days) and 25 °C (0, 3, 6, and 9 h), while day 0 was used as a control. The E-nose measurements were analyzed by Principal Component Analysis (PCA), Linear Discriminant Analysis (LDA) and Artificial Neural Network (ANN). Microbial counts increased significantly and simultaneously with the changes in E-nose measurements during storage. The LDA and ANN showed a good classification of E-nose data for different storage times at two storage temperatures (0 °C and 25 °C) compared to PCA. It is expected as PCA is based on linear relationships between the factors, while ANN is based on non-linear relationships. Correlation coefficients between E-nose and TBC, TMAO-reducing bacterial and H2S-producing bacterial counts at 0 °C were 0.919, 0.960 and 0.915, respectively, whereas at 25 °C, the correlation coefficients were 0.859, 0.945 and 0.849, respectively. These positive correlations qualify the E-nose as an efficient and reliable device for detecting microbial spoilage of fish during storage.
Investigation on 3D Printing of Shrimp Surimi Adding Three Edible Oils
Authors: Pan Yanmo , Sun Qinxiu , Liu Yang , Wei Shuai , Han Zongyuan , Zheng Ouyang , Ji Hongwu , Zhang Bin , Liu Shucheng
Published: January 2024

Three-dimensional (3D) printing provides a new method for innovative processing of shrimp surimi. However, there still exists a problem of uneven discharge during the 3D printing of surimi. The effects of different amounts of lard oil (LO), soybean oil (SO), and olive oil (OO) (0%, 2%, 4%, and 6%, respectively) added to shrimp surimi on the 3D printability of surimi were evaluated. The findings showed that with the increase in the added oil, the rheological properties, texture properties, water-holding capacity (WHC), and water distribution of surimi with the same kind of oil were significantly improved; the printing accuracy first increased and then decreased; and the printing stability showed an increasing trend (p < 0.05). The surimi with 4% oil had the highest printing adaptability (accuracy and stability). Different kinds of oil have different degrees of impact on the physical properties of surimi, thereby improving 3D-printing adaptability. Among all kinds of oil, LO had the best printing adaptability. In addition, according to various indicators and principal component analysis, adding 4% LO to shrimp surimi gave the best 3D-printing adaptability. But from the aspects of 3D printing properties and nutrition, adding 4% SO was more in line with the nutritional needs of contemporary people.
Optimization of 4,6-α and 4,3-α-Glucanotransferase Production in Lactococcus lactis and Determination of Their Effects on Some Quality Characteristics of Bakery Products
Authors: Niçin Ramazan Tolga , Zehir-Şentürk Duygu , Özkan Busenur , Göksungur Yekta , Şimşek Ömer
Published: January 2024

In this study, the production of 4,6-α (4,6-α-GTase) and 4,3-α-glucanotransferase (4,3-α-GTase), expressed previously in Lactococcus lactis, was optimized and these enzymes were used to investigate glycemic index reduction and staling delay in bakery products. HP–SEC analysis showed that the relevant enzymes were able to produce oligosaccharides from potato starch or malto-oligosaccharides. Response Surface Methodology (RSM) was used to optimize enzyme synthesis and the highest enzyme activities of 15.63 ± 1.65 and 19.01 ± 1.75 U/mL were obtained at 1% glucose, pH 6, and 30 °C for 4,6-α-GTase and 4,3-α-GTase enzymes, respectively. SEM analysis showed that both enzymes reduced the size of the starch granules. These enzymes were purified by ultrafiltration and used to produce bread and bun at an enzyme activity of 4 U/g, resulting in a decrease in the specific volume of the bread. It was found that the estimated glycemic index (eGI) of bread formulated with 4,6-α-GTase decreased by 18.01%, and the eGI of bread prepared with 4,3-α-GTase decreased by 13.61%, indicating a potential delay in staling. No significant differences were observed in the sensory properties of the bakery products. This is the first study showing that 4,6-α-GTase and 4,3-α-GTase enzymes have potential in increasing health benefits and improving technological aspects regarding bakery products.
Quality and Process Optimization of Infrared Combined Hot Air Drying of Yam Slices Based on BP Neural Network and Gray Wolf Algorithm
Authors: Zhang Jikai , Zheng Xia , Xiao Hongwei , Shan Chunhui , Li Yican , Yang Taoqing
Published: January 2024

In this paper, the effects on drying time (Y1), the color difference (Y2), unit energy consumption (Y3), polysaccharide content (Y4), rehydration ratio (Y5), and allantoin content (Y6) of yam slices were investigated under different drying temperatures (50–70 °C), slice thicknesses (2–10 mm), and radiation distances (80–160 mm). The optimal drying conditions were determined by applying the BP neural network wolf algorithm (GWO) model based on response surface methodology (RMS). All the above indices were significantly affected by drying conditions (p < 0.05). The drying rate and effective water diffusion coefficient of yam slices accelerated with increasing temperature and decreasing slice thickness and radiation distance. The selection of lower temperature and slice thickness helped reduce the energy consumption and color difference. The polysaccharide content increased and then decreased with drying temperature, slice thickness, and radiation distance, and it was highest at 60 °C, 6 mm, and 120 mm. At 60 °C, lower slice thickness and radiation distance favored the retention of allantoin content. Under the given constraints (minimization of drying time, unit energy consumption, color difference, and maximization of rehydration ratio, polysaccharide content, and allantoin content), BP-GWO was found to have higher coefficients of determination (R2 = 0.9919 to 0.9983) and lower RMSEs (reduced by 61.34% to 80.03%) than RMS. Multi-objective optimization of BP-GWO was carried out to obtain the optimal drying conditions, as follows: temperature 63.57 °C, slice thickness 4.27 mm, radiation distance 91.39 mm, corresponding to the optimal indices, as follows: Y1 = 133.71 min, Y2 = 7.26, Y3 = 8.54 kJ·h·kg−1, Y4 = 20.73 mg/g, Y5 = 2.84 kg/kg, and Y6 = 3.69 μg/g. In the experimental verification of the prediction results, the relative error between the actual and predicted values was less than 5%, proving the model’s reliability for other materials in the drying technology process research to provide a reference.
Antibacterial Effect and Possible Mechanism of Sesamol against Foodborne Pathogens
Authors: Li Zhuosi , Wu Mengjie , Yan Hui , Meng Zheyan , Gao Binru , Dong Qingli
Published: January 2024

Food safety problems caused by foodborne pathogens have become a major public issue, and the search for efficient and safe bacteriostatic agents has gained attention. Sesamol (SE), a phenolic compound abundant in sesame oil, offers numerous health benefits and exhibits certain antibacterial properties. The purpose of this study was to evaluate the antibacterial effect and potential mechanisms of SE against representative foodborne pathogens, including Listeria monocytogenes, Staphylococcus aureus, Bacillus cereus, Escherichia coli, and Salmonella serovar Enteritidis. The results showed that SE significantly inhibited the growth of the five pathogenic bacteria in sterile saline and pasteurized milk by 2.16–4.16 log10 CFU/g within 48 h. The results of the minimum bactericidal concentration and time–kill assay showed that SE had a greater inhibitory effect on L. monocytogenes compared with other bacteria. Additionally, SE was found to alter the cell membranes’ permeability in these bacteria, resulting in the release of intercellular proteins and DNA. A scanning electron microscopy analysis showed that exposure to SE resulted in significant changes in bacterial morphology, producing cell shrinkage and deformation. These findings suggest that SE could inhibit both Gram-negative and Gram-positive bacteria by interfering with the function and morphology of bacterial cells.
Sensing the Snacking Experience: Bodily Sensations Linked to the Consumption of Healthy and Unhealthy Snack Foods—A Comparison between Body Mass Index Levels
Authors: Frederiksen Chanette , Byrne Derek Victor , Andersen Barbara Vad
Published: January 2024

The World Health Organisation (WHO) has highlighted the need for immediate action regarding the escalating global prevalence of overweight and obesity. Based on the need for long-term strategies supporting dietary behaviour changes, the concept of ‘interoception’ (i.e., sensing the body’s internal state) has been proposed as promising for understanding and controlling food intake behaviours. As eating behaviours are motivated by the need for securing energy demands as well as the desire for pleasure, investigating the bodily sensations perceived in relation to food consumption can support our understanding of human food and eating behaviours. Here, a consumer study was conducted on 286 Danish consumers to explore the interoceptive experience of snack food consumption. This study included an investigation of the consumers’ interoceptive capabilities and ability to feel pleasure, intuitive eating behaviours, snack food consumption frequency, the drivers of snack food consumption, food-related pleasure, and post-ingestive sensations linked to snack food consumption. The study was conducted on consumers with different Body Mass Index (BMI) levels to study potential differences between these groups. The study showed normal interoceptive capabilities and abilities to feel pleasure, with no differences between BMI groups. Regarding intuitive eating, the study found intuitive eating to be more prevalent among the group holding normal BMI. No significant differences between BMI groups were found in terms of snack intake frequency. However, differences between BMI groups were found in terms of: the drivers of snack consumption, the aspects bringing food-related pleasure from snack consumption, and the post-ingestive sensations felt after eating snack foods.
Harnessing the Potential of Chitosan and Its Derivatives for Enhanced Functionalities in Food Applications
Authors: Yi Kexin , Miao Shiyuan , Yang Bixing , Li Sijie , Lu Yujie
Published: January 2024

As one of the most abundant natural polysaccharides that possess good biological activity, chitosan is extracted from chitin. Its application in the food field is being increasingly valued. However, chitosan extraction is difficult, and its poor solubility limits its application. At present, the extraction methods include the acid–base method, new chemical methods, and biological methods. The extraction rates of chitin/chitosan are 4–55%, 13–14%, and 15–28%, respectively. Different chemical modifications have different effects on chitosan, making it applicable in different fields. This article reviews and compares the extraction and chemical modification methods of chitosan, emphasizing the importance of green extraction methods. Finally, the application prospects of chitosan in the food industry are discussed. This will promote the understanding of the advantages and disadvantages of different extraction methods for chitosan as well as the relationship between modification and application, providing valuable insights for the future development of chitosan.
Valorization of Spent Grains from Beer Production through β-Glucan Extraction
Authors: Jantason Natcha , Suphantharika Manop , Wipatanawin Angkana , Chansong Suwan , Payongsri Panwajee
Published: January 2024

Brewers’ spent grains (BSG) are the major byproduct of the brewing industry. Recently, it has been found that β-glucan, which can be used as a food supplement, can be extracted from BSG and offers the greatest added value. This study aimed to investigate the effects of temperature (45–90 °C) and time (30–120 min) on β-glucan extraction efficiency when using hot water extraction. β-glucan was precipitated upon 80% ethanol addition. The chemical compositions were examined. The highest β-glucan concentration and yield were obtained at a temperature and time of 60 °C and 90 min, respectively. The functional properties of the extracted β-glucan were analyzed and compared with other commercial stabilizers such as sodium carboxymethyl cellulose (CMC), xanthan gum, gum arabic, and oat β-glucan. All stabilizers exhibited non-Newtonian flow behavior, except for gum arabic, which exhibited Newtonian flow behavior. The water holding capacity of BSG β-glucan was 6.82 g/g and the creaming index of the emulsions stabilized with BSG β-glucan was 89.05%. BSG β-glucan improved the color and stability of orange juice by reducing the precipitation of orange pulp. This study illustrated that BSG β-glucan can be used as a stabilizer and viscosity enhancer in foods, depending on the concentration, which can be applied to a variety of foods.
Bioactive Edible Gel Films Based on Wheat Flour and Glucose for Food Packaging Applications
Authors: Petaloti Argyri-Ioanna , Makri Styliani , Achilias Dimitris S.
Published: January 2024

In order to prepare bioactive edible gel films with enhanced properties, the feasibility of using wheat flour as a raw material with glucose added at several concentrations was studied in this investigation. Films were prepared with glucose concentrations of 0.5, 0.7 and 1 g/g of flour and characterized for their physicochemical properties, including water content, solubility, degree of swelling, chemical structure by FT-IR (ATR) spectroscopy, morphology by SEM microscopy, thermal properties by DSC, gas and water vapor permeability and antioxidant activity. Biodegradation studies were also carried out in soil for 27 days and evaluated by weight loss measurements. It was found that the gel film with the higher glucose concentration exhibits a homogeneous and continuous structure with no cracks and no fragility, accompanied by an increased thickness and solubility and a decreased degree of swelling compared to those with lower concentrations. The chemical structure of all films was verified. Moreover, the increase in glucose content leads to better gas barrier properties with lower oxygen, CO2 and water vapor transmission rates and increased water vapor permeability. A slightly elevated melting temperature was observed in the films with higher glucose content. Higher antioxidant activity was also associated with higher percentage of glucose. Finally, the biodegradation of the films ranged from 13 to nearly 70%. Therefore, it can be concluded that the addition of glucose to wheat flour in concentration up to 1 g/g could result in edible gel films with excellent properties to be used in food packaging applications.