bluuebunny/crossref_metadata_2025 huggingface dataset:
Pathogenicity of Seneca Valley virus in pigs and detection in Culicoides from an infected pig farm
Authors: Zhang Jinyong , Li Chenghui , Meng Yuan , Xie Yubiao , Shi Ning , Zhang He , Yu Chengdong , Nan Fulong , Xie Changzhan , Ha Zhuo , Han Jicheng , Li Zhuoxin , Li Qiuxuan , Wang Peng , Gao Xu , Jin Ningyi , Lu Huijun
Published: October 2021

Background

Porcine vesicular disease is caused by the Seneca Valley virus (SVV), it is a novelPicornaviridae,which is prevalent in several countries. However, the pathogenicity of SVV on 5–6 week old pigs and the transmission routes of SVV remain unknown.

Methods

This research mainly focuses on the pathogenicity of the CH-GX-01-2019 strain and the possible vector of SVV. In this study, 5–6 week old pigs infected with SVV (CH-GX-01-2019) and its clinical symptoms (including rectal temperatures and other clinical symptoms) were monitored, qRT-PCR were used to detect the viremia and virus distribution. Neutralization antibody assay was set up during this research. Mosquitoes andCulicoideswere collected from pigsties after pigs challenge with SVV, and SVV detection within mosquitoes andCulicoideswas done via RT-PCR.

Results

The challenged pigs presented with low fevers and mild lethargy on 5–8 days post infection. The viremia lasted more than 14 days. SVV was detected in almost all tissues on the 14th day following the challenge, and it was significantly higher in the hoofs (vesicles) and lymph nodes in comparison with other tissues. Neutralizing antibodies were also detected and could persist for more than 28 days, in addition neutralizing antibody titers ranged from 1:128 to 1:512. Mosquitoes andCulicoideswere collected from the pigsty environments following SVV infection. Although SVV was not detected in the mosquitoes, it was present in theCulicoides, however SVV could not be isolated from the positiveCulicoides.

Conclusions

Our work has enriched the knowledge relating to SVV pathogenicity and possible transmission routes, which may lay the foundation for further research into the prevention and control of this virus.
Neutrophils in cancer carcinogenesis and metastasis
Authors: Xiong Shumin , Dong Liaoliao , Cheng Lin
Published: October 2021

In recent years, neutrophils have attracted increasing attention because of their cancer-promoting effects. An elevated neutrophil-to-lymphocyte ratio is considered a prognostic indicator for patients with cancer. Neutrophils are no longer regarded as innate immune cells with a single function, let alone bystanders in the pathological process of cancer. Their diversity and plasticity are being increasingly recognized. This review summarizes previous studies assessing the roles and mechanisms of neutrophils in cancer initiation, progression, metastasis and relapse. Although the findings are controversial, the fact that neutrophils play a dual role in promoting and suppressing cancer is undeniable. The plasticity of neutrophils allows them to adapt to different cancer microenvironments and exert different effects on cancer. Given the findings from our own research, we propose a reasonable hypothesis that neutrophils may be reprogrammed into a cancer-promoting state in the cancer microenvironment. This new perspective indicates that neutrophil reprogramming in the course of cancer treatment is a problem worthy of attention. Preventing or reversing the reprogramming of neutrophils may be a potential strategy for adjuvant cancer therapy.
In vivo staging of regional amyloid progression in healthy middle-aged to older people at risk of Alzheimer’s disease
Authors: Levin Fedor , Jelistratova Irina , Betthauser Tobey J. , Okonkwo Ozioma , Johnson Sterling C. , Teipel Stefan J. , Grothe Michel J.
Published: October 2021

Background

We investigated regional amyloid staging characteristics in 11C-PiB-PET data from middle-aged to older participants at elevated risk for AD enrolled in the Wisconsin Registry for Alzheimer’s Prevention.

Methods

We analyzed partial volume effect-corrected 11C-PiB-PET distribution volume ratio maps from 220 participants (mean age = 61.4 years, range 46.9–76.8 years). Regional amyloid positivity was established using region-specific thresholds. We used four stages from the frequency-based staging of amyloid positivity to characterize individual amyloid deposition. Longitudinal PET data was used to assess the temporal progression of stages and to evaluate the emergence of regional amyloid positivity in participants who were amyloid-negative at baseline. We also assessed the effect of amyloid stage on longitudinal cognitive trajectories.

Results

The staging model suggested progressive accumulation of amyloid from associative to primary neocortex and gradually involving subcortical regions. Longitudinal PET measurements supported the cross-sectionally estimated amyloid progression. In mixed-effects longitudinal analysis of cognitive follow-up data obtained over an average period of 6.5 years following the baseline PET measurement, amyloid stage II showed a faster decline in executive function, and advanced amyloid stages (III and IV) showed a faster decline across multiple cognitive domains compared to stage 0.

Conclusions

Overall, the 11C-PiB-PET-based staging model was generally consistent with previously derived models from 18F-labeled amyloid PET scans and a longitudinal course of amyloid accumulation. Differences in longitudinal cognitive decline support the potential clinical utility of in vivo amyloid staging for risk stratification of the preclinical phase of AD even in middle-aged to older individuals at risk for AD.
Predictive ability of CT findings in the differentiation of complicated and uncomplicated appendicitis: a retrospective investigation of 201 patients undergone appendectomy at initial admission
Authors: Iamwat Jidapa , Teerasamit Wanwarang , Apisarnthanarak Piyaporn , Noppakunsomboon Napakadol , Kaewlai Rathachai
Published: October 2021

Background

Paradigm shift toward nonoperative management (NOM) of adult appendicitis has made computed tomography (CT) more important than ever, particularly in differentiating complicated from uncomplicated disease. Complete surgical and pathological data of appendicitis in a place where appendectomy at initial admission is a standard of care would allow retrospective review of preoperative CT for performance and predictive ability in identifying those that may benefit from NOM in the future.

Results

The study included 201 CT scans of consecutive adult patients who presented for appendectomy at initial admission with pathologically confirmed acute appendicitis. Complicated appendicitis referred to gangrene or perforation on pathological or operative findings. The overall CT sensitivity, specificity and accuracy for differentiation of complicated from uncomplicated appendicitis were 87.2%, 75.7% and 81.1%, respectively. The most sensitive CT findings of complicated appendicitis were mucosal enhancement defect (83.2%; 95% CI 74.1–90.0) and moderate-to-severe periappendiceal fat stranding (96.8%; 95% CI 91.1–99.3), both independently predictive of complicated appendicitis with adjusted odds ratios (ORs) of 4.62 (95% CI 1.86–11.51) and 4.41 (95% CI 1.06–18.29), respectively. Phlegmon, fluid collection, extraluminal appendicolith, periappendiceal air and small bowel dilatation had specificity of 98.1–100%. Intraluminal appendicoliths were found more frequently in complicated appendicitis (52.6% vs. 22.6%) but not predictive for this diagnosis. Independent clinical predictors of complicated appendicitis were lack of pain migration (OR 2.06), neutrophilia ≥ 82% (OR (2.87) and symptoms ≥ 24 h (OR 5.84).

Conclusions

CT findings were highly accurate in differentiating complicated from uncomplicated appendicitis among patients undergone appendectomy at initial admission.
2D-shear wave elastography: number of acquisitions can be reduced according to clinical setting
Authors: Dioguardi Burgio Marco , Grégory Jules , Ronot Maxime , Sartoris Riccardo , Chatellier Gilles , Vilgrain Valérie ,   the group SSI-SWE, Herrmann Eva , de Lédinghen Victor , Cassinotto Christophe , Chu Winnie C.-W. , Leung Vivian Y.-F. , Ferraioli Giovanna , Filice Carlo , Castera Laurent , Dumortier Jérôme , Guibal Aymeric , Pol Stanislas , Trebicka Jonel , Jansen Christian , Strassburg Christian , Zheng Rongqin , Zheng Jian , Francque Sven , Vanwolleghem Thomas , Vonghia Luisa , Manesis Emanuel K. , Zoumpoulis Pavlos , Sporea Ioan , Thiele Maja , Krag Aleksander , Friedrich-Rust Mireen
Published: October 2021

Background

The factors affecting intra-operator variability of two-dimensional shear wave elastography (2D-SWE) have not been clearly established. We evaluated 2D-SWE variability according to the number of measurements, clinical and laboratory features, and liver stiffness measurements (LSM).

Methods

At least three LSM were performed in 452 patients who underwent LSM by 2D-SWE (supersonic shear imaging) out of an initial database of 1650 patients. The mean value of the three LSM was our best measurement method. Bland–Altman plots were used to evaluate intra-operator variability when considering only one, or the first two measurements. Variability was assessed by taking the absolute value of the difference between the first LSM and the mean of the three LSM. Logistic regression was used to assess the factors associated with the highest tertile of variability.

Results

The limit of agreement was narrower with the mean of the first and second measurements than with each measurement taken separately (− 2.83 to 2.99 kPa vs. − 5.86 to 6.21 kPa and − 5.77 to 5.73 kPa for the first and second measurement, respectively). A BMI ≥ 25 kg/m2 and a first LSM by 2D-SWE ≥ 7.1 kPa increased the odds of higher variability by 3.4 and 3.9, respectively. Adding a second LSM didn’t change the variability in patients with BMI < 25 and a first LSM by 2D-SWE < 7.1 kPa.

Conclusions

Intra-operator variability of LSM by 2D-SWE increases with both a high BMI and high LSM value. In patients with BMI < 25 kg/m2 and a first LSM < 7.1 kPa we recommend performing only one LSM.
Readability of patient education materials related to radiation safety: What are the implications for patient-centred radiology care?
Authors: Delaney Francis T. , Doinn Tiarnán Ó. , Broderick James M. , Stanley Emma
Published: October 2021

Background

Increasing numbers of patients and carers rely on online resources for healthcare information. Radiation safety can be misunderstood by patients and clinicians and lead to patient anxiety. We aimed to assess the readability of online patient educational materials (PEMs) related to radiation safety.

Methods

A total of 84 articles pertaining to radiation safety from 14 well-known online resources were identified. PEMs were then analysed using Readability Studio Professional Edition Version 2019. Readability was assessed using eight different instruments: the Flesch-Kincaid Reading Grade Level, Raygor Estimate, SMOG, Coleman–Liau, Fry, FORCAST, Gunning Fog, and Flesch Reading Ease Score formula. The mean reading grade level (RGL) of each article was compared to the 6th and 8th grade reading level using 1-sample t-tests.

Results

The cumulative mean RGL for all 84 articles was 13.3 (range = 8.6–17.4), and none were written at or below the 6th or 8th grade level. The cumulative mean RGL exceeded the 6th grade reading level by an average of 7.3 levels (95% CI, 6.8–7.8; p < 0.001) and the 8th grade level by an average of 5.3 grade levels (95% CI, 4.8–5.8; p < 0.001). The mean Flesch Reading Ease Score was 39/100 (‘difficult’).

Conclusion

Currently available online PEMs related to radiation safety are still written at higher than recommended reading levels. Radiation safety is a topic in which the specialist training of radiologists is crucial in providing guidance to patients. Addressing the readability of online PEMs can improve radiology-patient communication and support the shift to a patient-centred model of practice.
Magnetic resonance imaging in diagnosis of indeterminate breast (BIRADS 3 & 4A) in a general population
Authors: Hernández Liliana , Díaz Gloria M. , Posada Catalina , Llano-Sierra Alejandro
Published: October 2021

Objective

Currently, mammography and ultrasonography are the most used imaging techniques for breast cancer screening. However, these examinations report many indeterminate studies with a low probability of being malignant, i.e., BIRADS 3 and 4A. This prospective study aims to evaluate the value of breast magnetic resonance imaging (MRI) to clarify the BIRADS categorization of indeterminate mammography or ultrasonography studies.

Methods

MRI studies acquired prospectively from 105 patients previously classified as BIRADS 3 or 4A were analyzed independently by four radiologists with different experience levels. Interobserver agreement was determined by the first-order agreement coefficient (AC1), and divergent results were re-analyzed for consensus. The possible correlation between the MRI and the mammography/ultrasound findings was evaluated, and each study was independently classified in one of the five BIRADS categories (BIRADS 1 to 5). In lesions categorized as BIRADS 4 or 5 at MRI, histopathological diagnosis was established by image-guided biopsy; while short-term follow-up was performed in lesions rated as BIRADS 3.

Results

Breast MRI was useful in diagnosing three invasive ductal carcinomas, upgraded from BIRADS 4A to BIRADS 5. It also allowed excluding malignancy in 86 patients (81.9%), avoiding 22 unnecessary biopsies and 64 short-term follow-ups. The MRI showed good diagnostic performance with the area under roc curve, sensitivity, specificity, PPV, and NPV of 0.995, 100%, 83.5%, 10.5%, and 100%, respectively.

Conclusions

MRI showed to be useful as a problem-solving tool to clarify indeterminate findings in breast cancer screening and avoiding unnecessary short-follow-ups and percutaneous biopsies.
Single-center versus multi-center biparametric MRI radiomics approach for clinically significant peripheral zone prostate cancer
Authors: Bleker Jeroen , Yakar Derya , van Noort Bram , Rouw Dennis , de Jong Igle Jan , Dierckx Rudi A. J. O. , Kwee Thomas C. , Huisman Henkjan
Published: October 2021

Objectives

To investigate a previously developed radiomics-based biparametric magnetic resonance imaging (bpMRI) approach for discrimination of clinically significant peripheral zone prostate cancer (PZ csPCa) using multi-center, multi-vendor (McMv) and single-center, single-vendor (ScSv) datasets.

Methods

This study’s starting point was a previously developed ScSv algorithm for PZ csPCa whose performance was demonstrated in a single-center dataset. A McMv dataset was collected, and 262 PZ PCa lesions (9 centers, 2 vendors) were selected to identically develop a multi-center algorithm. The single-center algorithm was then applied to the multi-center dataset (single–multi-validation), and the McMv algorithm was applied to both the multi-center dataset (multi–multi-validation) and the previously used single-center dataset (multi–single-validation). The areas under the curve (AUCs) of the validations were compared using bootstrapping.

Results

Previously the single–single validation achieved an AUC of 0.82 (95% CI 0.71–0.92), a significant performance reduction of 27.2% compared to the single–multi-validation AUC of 0.59 (95% CI 0.51–0.68). The new multi-center model achieved a multi–multi-validation AUC of 0.75 (95% CI 0.64–0.84). Compared to the multi–single-validation AUC of 0.66 (95% CI 0.56–0.75), the performance did not decrease significantly (p value: 0.114). Bootstrapped comparison showed similar single-center performances and a significantly different multi-center performance (p values: 0.03, 0.012).

Conclusions

A single-center trained radiomics-based bpMRI model does not generalize to multi-center data. Multi-center trained radiomics-based bpMRI models do generalize, have equal single-center performance and perform better on multi-center data.
A small molecule modulating monounsaturated fatty acids and Wnt signaling confers maintenance to induced pluripotent stem cells against endodermal differentiation
Authors: Hosseini Vahid , Kalantary-Charvadeh Ashkan , Hajikarami Maryam , Fayyazpour Parisa , Rahbarghazi Reza , Totonchi Mehdi , Darabi Masoud
Published: October 2021

Background

Stearoyl-coenzyme A desaturase 1 (SCD1) is required for de novo synthesis of fatty acids. Through the fatty acid acylation process, this enzyme orchestrates post-translational modifications to proteins involved in cell development and differentiation. In this study, we used biochemical methods, immunostaining, and covalent labeling to evaluate whether a small molecule modulating unsaturated fatty acids can influence the early endodermal differentiation of human-induced pluripotent stem cells (iPSCs).

Methods

The hiPSCs were cultured in an endoderm-inducing medium containing activin A and defined fetal bovine serum in the presence of an SCD1 inhibitor at different time points. The cell cycles and the yields of the three germ layers (endoderm, mesoderm, and ectoderm) were assessed using flow cytometry. The expression of endoderm and pluripotency markers and the expressions of Wnt signaling pathway proteins were assessed using western blotting and RT-PCR. Total protein acylation was evaluated using a click chemistry reaction.

Results

When SCD1 was inhibited on the first day, the population of cells with endodermal features decreased at the end of differentiation. Moreover, early SCD1 inhibition preserved the properties of hiPSCs, preventing their shift toward mesodermal or ectodermal lineage. Also, first-day-only treatment of cells with the SCD1 inhibitor decreased β-catenin gene expression and the intensity of fluorescent emission in the click chemistry assay. The cells were effectively rescued from these effects by cotreatment with oleate. Late treatment with the inhibitor in the two subsequent days of endoderm induction did not have any significant effects on endoderm-specific markers or fluorescent intensity. Reproducible results were also obtained with human embryonic stem cells.

Conclusion

The small molecule SCD1 inhibitor attenuates the Wnt/β-catenin signaling pathway, conferring the maintenance of hiPSCs by opposing the initiation of endoderm differentiation. The immediate requirement for SCD1 activity in the endoderm commitment of pluripotent stem cells may be of importance in disorders of endoderm-derived organs and dysregulated metabolism.

The schematic representation of the study design and main results. Activin A induces endoderm features through Smad2/3/4 and increases the expression of SCD1. SCD1 can produce MUFAs and subsequently modify the Wnt molecules. MUFA acylated/activated Wnts are secreted to interact with corresponding receptors on the target cells. β-catenin accumulates in the cytoplasm and is translocated into the nucleus after the interaction of Wnt with the receptor. Then, β-catenin increases the expression of the endoderm markers Sox17 and CXCR4.
Glaesserella parasuis serotype 4 HPS4-YC disrupts the integrity of the swine tracheal epithelial barrier and facilitates bacterial translocation
Authors: Wang Qing , Chang Xiaojing , Liu Mingxing , Lu Qi , Zhu Meng , Lin Huixing , Fan Hongjie
Published: October 2021

Glaesserella parasuis (G. parasuis) is a commensal bacterium in the upper respiratory tract of pigs that can also cause the swine Glässer disease, which induces an intensive inflammatory response and results in significant economic losses to the swine industry worldwide. G. parasuis can cause disease through infection of the respiratory tract, resulting in systemic infection, but the mechanism is largely unknown. Recently we showed that Glaesserella parasuis serotype 4 (GPS4) increased swine tracheal epithelial barrier permeability, resulting in easier bacterial translocation. Tight junction proteins (TJ) play a crucial role in maintaining the integrity and impermeability of the epithelial barrier. GPS4 decreased the expression of the TJ ZO-1 and occludin in swine tracheal epithelial cells (STEC). Furthermore, the proinflammatory cytokines IL-6, IL-8 and TNF-α were significantly upregulated in GPS4-infected STEC, and both the MAPK and NF-κB signaling pathways were activated and contributed to the expression of TNF-α. We demonstrate that the production of proinflammatory cytokines, especially TNF-α, during GPS4 infection was involved in barrier dysfunction. Additionally, animal challenge experiments confirmed that GPS4 infection downregulated TJ in the lungs of piglets and induced a severe inflammatory response. In general, G. parasuis infection downregulated the expression of TJ and induced massive secretion of proinflammatory cytokines, resulting in epithelial barrier disruption and favoring bacterial infection. This study allowed us to better understand the mechanism by which G. parasuis crosses the respiratory tract of pigs.
A pre-pandemic COVID-19 assessment of the costs of prevention and control interventions for healthcare associated infections in medical and surgical wards in Québec
Authors: Tchouaket Nguemeleu Eric , Robins Stephanie , Boivin Sandra , Sia Drissa , Kilpatrick Kelley , Dubreuil Bruno , Larouche Catherine , Parisien Natasha , Letourneau Josiane
Published: October 2021

Background

Healthcare-associated infections (HCAIs) present a major public health problem that significantly affects patients, health care providers and the entire healthcare system. Infection prevention and control programs limit HCAIs and are an indispensable component of patient and healthcare worker safety. The clinical best practices (CBPs) of handwashing, screening, hygiene and sanitation of surfaces and equipment, and basic and additional precautions (e.g., isolation, and donning and removing personal protective equipment) are keystones of infection prevention and control (IPC). There is a lack of rigorous IPC economic evaluations demonstrating the cost–benefit of IPC programs in general, and a lack of assessment of the value of investing in CBPs more specifically.

Objective

This study aims to assess overall costs associated with each of the four CBPs.

Methods

Across two Quebec hospitals, 48 healthcare workers were observed for two hours each shift, for two consecutive weeks. A modified time-driven activity-based costing framework method was used to capture all human resources (time) and materials (e.g. masks, cloths, disinfectants) required for each clinical best practice. Using a hospital perspective with a time horizon of one year, median costs per CBP per hour, as well as the cost per action, were calculated and reported in 2018 Canadian dollars ($). Sensitivity analyses were performed.

Results

A total of 1831 actions were recorded. The median cost of hand hygiene (N = 867) was 20 cents per action. For cleaning and disinfection of surfaces (N = 102), the cost was 21 cents per action, while cleaning of small equipment (N = 85) was 25 cents per action. Additional precautions median cost was $4.1 per action. The donning or removing or personal protective equipment (N = 720) cost was 76 cents per action. Finally, the total median costs for the five categories of clinical best practiced assessed were 27 cents per action.

Conclusions

The costs of clinical best practices were low, from 20 cents to $4.1 per action. This study provides evidence based arguments with which to support the allocation of resources to infection prevention and control practices that directly affect the safety of patients, healthcare workers and the public. Further research of costing clinical best care practices is warranted.
A quest for unrest in multiparameter observations at Whakaari/White Island volcano, New Zealand 2007–2018
Authors: Caudron Corentin , Girona Társilo , Jolly Arthur , Christenson Bruce , Savage Martha Kane , Carniel Roberto , Lecocq Thomas , Kennedy Ben , Lokmer Ivan , Yates Alexander , Hamling Ian , Park Iseul , Kilgour Geoff , Mazot Agnès
Published: October 2021

The Whakaari/White Island volcano, located ~ 50 km off the east coast of the North Island in New Zealand, has experienced sequences of quiescence, unrest, magmatic and phreatic eruptions over the last decades. For the last 15 years, seismic data have been continuously archived providing potential insight into this frequently active volcano. Here we take advantage of this unusually long time series to retrospectively process the seismic data using ambient noise and tremor-based methodologies. We investigate the time (RSAM) and frequency (Power Spectral Density) evolution of the volcanic tremor, then estimate the changes in the shallow subsurface using the Displacement Seismic Amplitude Ratio (DSAR), relative seismic velocity (dv/v) and decorrelation, and the Luni-Seismic Correlation (LSC). By combining our new set of observations with the long-term evolution of earthquakes, deformation, visual observations and geochemistry, we review the activity of Whakaari/White Island between 2007 and the end of 2018. Our analysis reveals the existence of distinct patterns related to the volcano activity with periods of calm followed by cycles of pressurization and eruptions. We finally put these results in the wider context of forecasting phreatic eruptions using continuous seismic records.
Survival and predictive factors in dialysis patients with COVID-19 in Japan: a nationwide cohort study
Authors: Kikuchi Kan , Nangaku Masaomi , Ryuzaki Munekazu , Yamakawa Tomoyuki , Yoshihiro Oota , Hanafusa Norio , Sakai Ken , Kanno Yoshihiko , Ando Ryoichi , Shinoda Toshio , Nakamoto Hidetomo , Akizawa Tadao ,   COVID-19 Task Force Committee of the Japanese Association of Dialysis Physicians, the Japanese Society for Dialysis Therapy, and the Japanese Society of Nephrology
Published: October 2021

Background

The Japanese Association of Dialysis Physicians, the Japanese Society for Dialysis Therapy, and the Japanese Society of Nephrology jointly established COVID-19 Task Force Committee and began surveying the number of newly infected patients.

Methods

This registry of the COVID-19 Task Force Committee was used to collect data of dialysis patients; a total of 1010 dialysis patients with COVID-19 were included in the analysis. Overall survival of patients was investigated with stratification by age group, complication status, and treatment. In addition, predictive factors for mortality were also investigated. The overall survival was estimated by Kaplan–Meier methods and compared by using log-rank test. Multivariate analysis was performed to identify the risk factor of mortality. For all statistical analyses, p < 0.05 was considered to be statistically significant.

Results

The mortality risk was increased with age (p < 0.001). The mortality risk was significantly higher in patients with peripheral arterial disease (HR: 1.49, 95% CI 1.05–2.10) and significantly lower in patients who were treated with remdesivir (HR: 0.60, 95% CI 0.37–0.98). Multivariate analysis showed increased risk of mortality with increment in BMI, and increment in CRP, and decreased risk with increment in albumin.

Conclusion

Dialysis patients have a high severity of illness and a high risk of mortality in cases of COVID-19. Treatment with remdesivir might be effective in shortening the duration of hospitalization and reducing the risk of mortality.
Design of switchable transient thermal concentrators with diamond shapes
Authors: Feng H. L. , Zhou L. M. , Ni Y. S.
Published: October 2021

Thermal concentrators designed by the transformation thermotics have recently received considerable attention on the aspect of improving thermal energy efficiency in the process of energy consumption. However, the existing researches of thermal concentrators with diamond shapes are limited to steady state and non-switchable state, which hinders the analysis of the changeable thermal concentrating effect with time and restricts practical applications under different temperature conditions. In order to overcome these limitations, the intelligent switchable transient thermal concentrators with diamond shapes are designed in this paper, which can realize the switchable concentrating effect as the temperature changes. The numerical simulation method is employed to demonstrate the temperature-dependent properties of switchable concentrators. The results show that the concentrator can achieve perfect performance of thermal concentrating at low (or high) temperature, and the concentrating effect is automatically turned off at high (or low) temperature. The switchable concentrating effect can greatly widen the application fields of thermal concentrators and provide an effective method for heat management, compared with the previous steady-state and non-switchable–state concentrators with diamond shapes.
Unexpected effect of an empty cavity on water transport through a combined carbon nanotube
Authors: Meng X. W. , Li Y.
Published: October 2021

Controlling water transport via an empty cavity can achieve unexpected properties for various applications. In this paper, water transport through a combined carbon nanotube with an empty cavity is studied by molecular dynamics simulations. It is found that the occupancy of water molecules and the transfer rate of water molecules through the combined carbon nanotube can be affected by the size of the cavity unexpectedly under a relatively low pressure difference. The occupancy of water molecules in the combined carbon nanotube and the average transfer rate begin to decrease significantly if the size of the cavity reaches a critical radius of 0.733 nm. The energy gap between water molecules and the combined carbon nanotube is adopted to explain the effect of the empty cavity on water properties in the combined carbon nanotube. Our findings may pave a way for understanding the effect of the empty cavity on water dynamics in the carbon nanotube and offering another way to control water properties at the nanoscale.
Genetically Predicted Lifelong Circulating 25(OH)D Levels are Associated With Serum Calcium Levels and Kidney Stone Risk
Authors: Jian Zhongyu , Huang Yu , He Yazhou , Jin Xi , Li Hong , Li Sheyu , Wang Kunjie
Published: October 2021

Objective

To assess whether lifelong higher circulating 25-hydroxyvitamin D [25(OH)D] levels increase serum calcium levels and kidney stone disease (KSD) risk.

Methods

Summary data for KSD were obtained from the UK biobank genome-wide association study (6536 cases and 388 508 controls). We acquired summary data for 25(OH)D from 120 618 Europeans and another large-scale analysis (443 734 Europeans) for primary and secondary analysis. Random-effect inverse-variance weighted (IVW) and 7 additional sensitivity analyses were applied. Next, multivariable Mendelian randomization (MVMR) was performed by introducing data for serum calcium levels.

Results

Genetic predisposition for a 1-SD higher 25(OH)D level was associated with increased serum calcium levels (IVW; beta, 0.014; 95% CI, 0.010-0.018; P = 7.64E-10). Genetically predicted higher circulating 25(OH)D levels were associated with increased the risk of KSD, with per 1-SD odds ratios (ORs) of 1.47 (95% CI, 1.22-1.77; P = 5.49E-05) and 1.36 (95% CI, 1.03-1.80; P = 0.029) using the IVW and MVMR-Egger methods, respectively. In secondary analysis, similar results were found: 25(OH)D was associated with an increased risk of KSD in univariate Mendelian randomization (IVW; OR 1.71; 95% CI, 1.26-2.32; P = 0.001) and MVMR (OR 1.43; 95% CI, 1.16-1.76; P < 0.001) analyses. Most sensitivity analyses were consistent with the primary results, both for the primary and secondary analyses.

Conclusions

Our study supports that higher genetically predicted lifelong circulating 25(OH)D levels are associated with higher calcium levels and KSD risk. The effects of 25(OH)D on KSD were partially attenuated—but still significant—in MVMR.
Distribution, diet and relationships of the invasive ctenophore Mnemiopsis leidyi with anchovies and zooplankton, in the northeastern Adriatic Sea
Authors: BUDIŠA ANDREA , PALIAGA PAOLO , JURETIĆ TEA , LUČIĆ DAVOR , SUPIĆ NASTJENJKA , PASARIĆ ZORAN , DJAKOVAC TAMARA , MLADINIĆ MARIJA , DADIĆ VLADO , TIČINA VJEKOSLAV
Published: October 2021

Blooms of invasive ctenophore Mnemiopsis leidyi can have massive consequences on fish stocks and marine food webs. The distribution, abundance and diet of this ctenophore were investigated in the northeastern (NE) Adriatic between 2016 and 2019. The abundance of M. leidyi was determined daily along the coast of Rovinj (Croatia), and its spatial distribution monitored by visual census from research vessels and by pelagic trawl during acoustic surveys in September of each year. Mesozooplankton samples were collected along the western coast of Istria by vertical tows from the bottom to the surface. Spatial distribution and abundance of anchovy (Engraulis encrasicolus) assemblages were determined by geo-referenced hydro-acoustic sampling using a scientific echosounder. Large swarms of M. leidyi covering several km2 were regularly reported between July and November extending up to 25 NM from the western coast of the Istrian peninsula and reaching maximum offshore densities of 270 individuals per m2. The abundance of anchovy in the areas where M. leidyi was present evidently decreased. The content of M. leidyi’s digestive tract and plankton samples consisted both mainly of cladocerans, copepods, pteropods, echinodermata and bivalvia larvae. Results indicate that M. leidyi may accumulate and increase its abundance in the stagnant and trophically rich areas of the northern Adriatic gyres, spreading to the surrounding larger areas with currents. We assume that M. leidyi presence correlates with a decrease in anchovy population due to competition for food (zooplankton). Our findings support the importance of implementing an international monitoring program throughout the Adriatic Sea and demonstrate the ability of current MEDIAS surveys to detect changes in the pelagic ecosystem throughout surveyed areas.
Factors affecting Oithona nana and Oithona similis along a salinity gradient
Authors: ANNABI-TRABELSI NEILA , KOBBI-REBAI RAYDA , AL-ENEZI YOUSEF , ALI MOHAMMAD , SUBRAHMANYAM M.N.V , BELMONTE GENUARIO , AYADI HABIB
Published: October 2021

Spatial and temporal variations of reproduction variables of the two cyclopoid copepods Oithona nana Giesbrecht, 1893 and Oithona similis Claus, 1866 were evaluated monthly from September 2007 to August 2008 in four ponds at different salinities in the solar saltern of Sfax, Tunisia, under hypersaline conditions. Along the salinity gradient the abundance of O. nana and O. similis declined from 14,900 ± 15,200 ind. m-3 (A1) to 6,800 ± 7,200 (C31) and from 13,800 ± 17,100 ind. m-3 (A1) to 3,700 ± 5,800 (C31), respectively. The findings showed that the abundance of these Oithona species, particularly the ovigerous females, was positively correlated with the abundance of Diatomeae, and negatively correlated with salinity. The egg production rate (EPR), the egg diameter, the clutch size, as well as the cephalothorax length of mature females, were all negatively correlated with salinity and positively correlated with the abundance of Diatomeae. Along the existing salinity gradient, the EPR decreased from 4.7 ± 0.39 (A1) to 3.2 ± 0.13 (C31) eggs female-1 day-1 and from 5.19 ± 0.46 (A1) to 3.66 ± 0.05 (C31) eggs female-1 day-1 for O. nana and O. similis, respectively, and this could mainly be attributed to the hypersaline conditions. The positive relationship between the abundance of Diatomeae with all traits shows, that these may be a vital component of the diet for the investigated species. The reduction of body size of Oithona along the salinity gradient demonstrates that such a character could mainly be due to a network of causes, related with the confinement and not only to salinity.
History of hydroacoustic surveys of small pelagic fish species in the European Mediterranean Sea
Authors: LEONORI IOLE , TIČINA VJEKOSLAV , GIANNOULAKI MARIANNA , HATTAB TAREK , IGLESIAS MAGDALENA , BONANNO ANGELO , COSTANTINI ILARIA , CANDUCI GIOVANNI , MACHIAS ATHANASSIOS , VENTERO ANA , SOMARAKIS STYLIANOS , TSAGARAKIS KONSTANTINOS , BOGNER DANIJELA , BARRA MARCO , BASILONE GUALTIERO , GENOVESE SIMONA , JURETIĆ TEA , GAŠPAREVIĆ DENIS , DE FELICE ANDREA
Published: October 2021

The study of small pelagic fish by hydroacoustic methods in the Mediterranean Sea began in the Adriatic in the 1950’s. Since 2009, internationally coordinated, extensive, regular surveys have been conducted in the framework of the MEDIAS - Mediterranean International Acoustic Surveys – action, under the EU Data Collection Framework, to provide inputs for the management of small pelagics, particularly European anchovy (Engraulis encrasicolus) and European sardine (Sardina pilchardus). The surveys cover EU Mediterranean waters and monitor the distribution and abundance of small pelagic species using a common protocol. The hydroacoustic surveys, historically conducted by each Country separately, have since been standardized and harmonized. This is a review of the evolution of the surveys from the beginning up to the present and discusses the future prospects. It reports the historical time series of anchovy, sardine and other small pelagics in different areas and describes the spatial and temporal distribution of small pelagic species in decadal maps of the major areas of their distribution in the European Mediterranean Sea. Furthermore, it discusses the use of MEDIAS data for stock assessment purposes, the collection of auxiliary information for an ecosystem-based management approach, the need for further standardization and future challenges.
Two-way bioinvasion: tracking the neritic non-native cyclopoid copepods Dioithona oculata and Oithona davisae (Oithonidae) in the Eastern Mediterranean Sea
Authors: VELASQUEZ XIMENA , MOROV ARSENIY R. , TERBIYIK KURT TUBA , MERON DALIT , GUY-HAIM TAMAR
Published: October 2021

Accelerated anthropogenic changes in the Eastern Mediterranean Sea (EMS) have facilitated the introduction, spread and establishment of invasive copepod species in this region. Here, we report the introduction of two non-native cyclopoid copepods Dioithona oculata and Oithona davisae for the first time in the Israeli coastal waters and describe their temporal variability. The species were identified by morphological characteristics, DNA barcoding and phylogenetic inference. Molecular identification and phylogenetic analysis supported the taxonomical identification, nevertheless, showed cryptic speciation within D. oculata, separating the Western Pacific and EMS clades. In the Israeli coastal waters, D. oculata presented a temporally restricted occurrence, appearing from September 2019 to December 2019 (30.0±0.7 – 21.0±1.1 °C) and October 2020 (28.0±0.7 °C). The highest abundances of D. oculata occurred in the autumn (October 2019 and 2020), when the water temperature reached 28.0 °C (7 and 10 ind. m-3, respectively). The lowest abundance occurred in December 2019 (0.35 ind. m-3), when the water temperature decreased to 21.0 °C, indicating that the thermal affinity of D. oculata for warm-temperate conditions, for reproduction and the maintenance of viable populations, has persisted in the introduced range. In contrast, O. davisae appeared almost all year around (17.0±0.5 – 28.0±0.7 °C). This species demonstrated peaks in abundance both in October 2019 and October 2020, when the water temperature reached 28.0 °C (406 and 92 ind. m-3), as well as when the temperature decreased to 17.0 °C (31 ind. m-3, February 2020), confirming its wide eurythermal tolerance. Based on our findings and previous observations, we suggest that D. oculata may have invaded the EMS through the Suez Canal and is now at the onset of its spread in the Mediterranean Sea, whereas O. davisae has been introduced via shipping, likely from the Northeast Atlantic, widely spreading and successfully establishing viable populations across the entire Mediterranean Sea, until the coastal Levantine Sea.
Nesting activity of the Loggerhead sea turtle, Caretta caretta, in Calabria: 2016-2020 reproductive seasons
Authors: DENARO MARIA , MALITO TERESA , MANCUSO CARMELA , PARISE GIOVANNI , URSO SALVATORE
Published: October 2021

The nesting activity of the loggerhead turtle along the coast of Calabria during five reproductive seasons (2016-2020) is presented. From May to August, survey methods were conducted using traditional observations on foot and utilizing additional innovative technologies, including electric fat bikes and drones. Monitoring was intensively focused on a key nesting area of approximately 40 km located on the southernmost Ionian coast of Reggio Calabria, and, for only the 2020 season, the area was extended an additional 40 km northeast. In the five nesting seasons, 419 female emergences were recorded, 192 of which were classified as nests. Overall, 65.1% (n = 125) of nests were found along the area regularly monitored in all nesting seasons. The maximum nesting activity was observed during July, and the mean nesting success was 45.8%. The average number of eggs for all seasons was 92.2 (n = 163; S.D.: ± 21.1; range: 39-160). The mean hatching and emergence success rates were 70.8% and 62.5%, respectively. The mean incubation period was 49 days (n = 122; S.D.: ± 5.4; range: 42-82). Considering previous data from the same area, our study shows an actual increase in nesting activity, probably due to the implementation of an effective monitoring method that includes the use of new technologies, which made it possible to expand the regularly monitored area, and a greater awareness campaign in recent years. Regarding the reproductive parameters, our data is similar to that reported for other Mediterranean nesting grounds.
Informing the general public on the threat status of the European spiny lobster, Palinurus elephas (Fabricius, 1787) through Citizen-Science and social media platforms. A case study from the Aegean Sea
Authors: KAMPOURIS THODOROS E. , KOUTSOUBAS DROSOS , KANELOPOULOU KATERINA , ZANNAKI CASSANDRA , BATJAKAS IOANNIS E.
Published: October 2021

Research known as Citizen Science (CS) has been recognized as an important tool for both biodiversity restoration and fisheries management projects through active involvement of citizens and therefore for public environmental awareness too. CS projects should be well designed and state clear questions, that will benefit both the research goals and the citizen scientists themselves. In January 2019, the “Red Fish Project” group was created on Facebook consisting of SCUBA divers and fishers and offering the main data source and the relevant digital material. The project’s establishment was based on previous personal experience and following specific protocols. Further information was provided by personal communications. Till October 21st, 2020 the FB group consisted of 464 members who recorded 348 European spiny lobster individuals from all sub-regions of the Aegean Sea. Based on the findings of the present study it seems that most of the citizens were unaware that the species is listed as “Vulnerable” in the IUCN Red List. Lastly, we discuss the important role of the spiny lobster in the country’s culture and that it can be listed as a charismatic, ﬂagship species for citizens to be ocean-literate since it has been shown, that it can raise the interest of recreational divers and increase their awareness.
An overview of bottom trawl selectivity in the Mediterranean Sea
Authors: LUCCHETTI ALESSANDRO , VIRGILI MASSIMO , VASAPOLLO CLAUDIO , PETETTA ANDREA , BARGIONE GIADA , LI VELI DANIEL , BRĈIC JURE , SALA ANTONELLO
Published: October 2021

In the Mediterranean Sea, where bottom trawling for demersal species is the most important fishery in terms of landings, around 75% of the assessed fish stocks are overfished. Its status as one of the world’s most heavily exploited seas and the one subject to the highest trawling pressure has become a global concern. An extensive review of bottom trawl selectivity studies was performed to assess the sustainability of this fishery in the Mediterranean; the selectivity parameters were collected from 93 peer-reviewed publications of 10 countries, totalling 742 records and 65 species. The review highlighted that i) the catch of bottom trawls commonly employed in the Mediterranean, even complying with current regulations on codend meshes, still includes immature individuals for 64-68% of the species investigated, and individuals under the minimum conservation reference size (MCRS) for 78% of the species investigated, and that ii) the MCRS set for 59% of the species analyzed is well below their length at first maturity, and is therefore ecologically inadequate. Although square-mesh codends are slightly more selective, the models developed herein demonstrate that improving size and species selectivity would require considerably larger meshes, which may significantly reduce profitability. The urgent need to reduce the biological impacts of bottom trawling in the Mediterranean should be addressed by promoting the adoption of more ecologically sustainable fishing gears through the introduction of more selective meshes or gear modifications.
Tracing the occurrence of ocean sciences issues in Greek secondary education textbooks
Authors: MOGIAS ATHANASIOS , BOUBONARI THEODORA , KEVREKIDIS THEODOROS
Published: October 2021

This study aims to investigate the presence of ocean sciences issues in Greek secondary education (grades 7-12) science textbooks, in respect of the Ocean Literacy Framework. Content analysis was undertaken concerning both textual and pictorial materials of the Biology, Chemistry, Physics, and Geography – Geology courses. Results revealed that the textbooks under study contain limited and fragmented information with regard to the seven essential principles of the framework, while a comparison with the Ocean Literacy Scope & Sequence evidences inconsistencies.  The suggestions arising from this study could help curriculum designers, textbook authors, marine educators, and marine scientists to cooperate on a wider scale towards the inclusion of ocean literacy topics into national curricula worldwide.
Prevalence study of intermittent hormonal therapy of Prostate Cancer patients in Spain
Authors: Bonfill-Cosp Xavier , Auladell-Rispau Ariadna , Gich Ignasi , Zamora Javier , Saiz Luis Carlos , Pijoan Jose Ignacio , Urreta Iratxe , Cordero José Antonio
Published: October 2021

Background: Although intermittent androgen deprivation therapy was introduced many years ago to improve patients’ quality of life with the same carcinologic efficiency as continuous hormonal therapy, recent data suggest that those patients could be overtreated. This study aims to estimate the prevalence of prostate cancer patients receiving intermittent androgen deprivation therapy in Spain.

Methods: A retrospective, longitudinal study was conducted using electronic drug dispensation data from four Spanish autonomous communities, which encompass 17.23 million inhabitants (36.22% of the total population in Spain). We estimated intermittent androgen therapy use (%IAD) and the prevalence of patients under intermittent androgen therapy (PIAD) overall and stratified by region. Other outcome variables included the pharmaceutical forms dispensed and the total direct annual expenditure on androgen deprivation therapy‐associated medications.

Results: A total of 863,005 dispensations corresponding to a total of 65,752 men were identified, treated with either luteinizing hormone-releasing hormone (LHRH) analogues (353,162) administered alone or in combination with anti‐androgens (509,843). Overall, the mean (±SD) age of the patients was 76.9 (±10.4) years. Results revealed that the mean annual PIAD along the study was 6.6% in the total population studied, and the overall %IAD during the five‐year study period was 5.6%. The mean cost of hormonal therapy per year was 25 million euros for LHRH analogues and 6.3 million euros for anti-androgens.

Conclusions:  An important proportion of prostate cancer patients in Spain could benefit from intermittent androgen therapy during the study period while avoiding overtreatment harms associated with continuous hormonal therapy.