bluuebunny/crossref_metadata_2025 huggingface dataset:
The Optimal Strategies to Be Adopted in Controlling the Co-Circulation of COVID-19, Dengue and HIV: Insight from a Mathematical Model
Authors: Omame Andrew , Raezah Aeshah A. , Diala Uchenna H. , Onuoha Chinyere
Published: August 2023

The pandemic caused by COVID-19 led to serious disruptions in the preventive efforts against other infectious diseases. In this work, a robust mathematical co-dynamical model of COVID-19, dengue, and HIV is designed. Rigorous analyses for investigating the dynamical properties of the designed model are implemented. Under a special case, the stability of the model’s equilibria is demonstrated using well-known candidates for the Lyapunov function. To reduce the co-circulation of the three diseases, optimal interventions were defined for the model and the control system was analyzed. Simulations of the model showed different control scenarios, which could have a positive or detrimental impact on reducing the co-circulation of the diseases. Highlights of the simulations included: (i) Upon implementation of the first intervention strategy (control against COVID-19 and dengue), it was observed that a significant number of single and dual infection cases were averted. (ii) Under the COVID-19 and HIV prevention strategy, a remarkable number of new single and dual infection cases were also prevented. (iii) Under the COVID-19 and co-infection prevention strategy, a significant number of new infections were averted. (iv) Comparing all the intervention measures considered in this study, it is possible to state that the strategy that combined COVID-19/HIV averted the highest number of new infections. Thus, the COVID-19/HIV strategy would be the ideal and optimal strategy to adopt in controlling the co-spread of COVID-19, dengue, and HIV.
Characterization of a Decellularized Sheep Pulmonary Heart Valves and Analysis of Their Capability as a Xenograft Initial Matrix Material in Heart Valve Tissue Engineering
Authors: İnal Müslüm Süleyman , Darcan Cihan , Akpek Ali
Published: August 2023

In order to overcome the disadvantages of existing treatments in heart valve tissue engineering, decellularization studies are carried out. The main purpose of decellularization is to eliminate the immunogenicity of biologically derived grafts and to obtain a scaffold that allows recellularization while preserving the natural tissue architecture. SD and SDS are detergent derivatives frequently used in decellularization studies. The aim of our study is to decellularize the pulmonary heart valves of young Merino sheep by using low-density SDS and SD detergents together, and then to perform their detailed characterization to determine whether they are suitable for clinical studies. Pulmonary heart valves of 4–6-month-old sheep were decellularized in detergent solution for 24 h. The amount of residual DNA was measured to determine the efficiency of decellularization. Then, the effect of decellularization on the ECM by histological staining was examined. In addition, the samples were visualized by SEM to determine the surface morphologies of the scaffolds. A uniaxial tensile test was performed to examine the effect of decellularization on biomechanical properties. In vitro stability of scaffolds decellularized by collagenase treatment was determined. In addition, the cytotoxic effect of scaffolds on 3T3 cells was examined by MTT assay. The results showed DNA removal of 94% and 98% from the decellularized leaflet and pulmonary wall portions after decellularization relative to the control group. No cell nuclei were found in histological staining and it was observed that the three-layer leaflet structure was preserved. As a result of the tensile test, it was determined that there was no statistically significant difference between the control and decellularized groups in the UTS and elasticity modulus, and the biomechanical properties did not change. It was also observed that decellularized sheep pulmonary heart valves had no cytotoxic effect. In conclusion, we suggest that the pulmonary valves of decellularized young Merino sheep can be used as an initial matrix in heart valve tissue engineering studies.
An Approach to Binary Classification of Alzheimer’s Disease Using LSTM
Authors: Salehi Waleed , Baglat Preety , Gupta Gaurav , Khan Surbhi Bhatia , Almusharraf Ahlam , Alqahtani Ali , Kumar Adarsh
Published: August 2023

In this study, we use LSTM (Long-Short-Term-Memory) networks to evaluate Magnetic Resonance Imaging (MRI) data to overcome the shortcomings of conventional Alzheimer’s disease (AD) detection techniques. Our method offers greater reliability and accuracy in predicting the possibility of AD, in contrast to cognitive testing and brain structure analyses. We used an MRI dataset that we downloaded from the Kaggle source to train our LSTM network. Utilizing the temporal memory characteristics of LSTMs, the network was created to efficiently capture and evaluate the sequential patterns inherent in MRI scans. Our model scored a remarkable AUC of 0.97 and an accuracy of 98.62%. During the training process, we used Stratified Shuffle-Split Cross Validation to make sure that our findings were reliable and generalizable. Our study adds significantly to the body of knowledge by demonstrating the potential of LSTM networks in the specific field of AD prediction and extending the variety of methods investigated for image classification in AD research. We have also designed a user-friendly Web-based application to help with the accessibility of our developed model, bridging the gap between research and actual deployment.
Dimethyl Sulfoxide Inhibits Bile Acid Synthesis in Healthy Mice but Does Not Protect Mice from Bile-Acid-Induced Liver Damage
Authors: Chen Xi , Li Huiqiao , Liu Yu’e , Qi Jing , Dong Bingning , Huang Shixia , Zhao Shangang , Zhu Yi
Published: August 2023

Bile acids serve a vital function in lipid digestion and absorption; however, their accumulation can precipitate liver damage. In our study, we probed the effects of dimethyl sulfoxide (DMSO) on bile acid synthesis and the ensuing liver damage in mice induced by bile acids. Our findings indicate that DMSO efficaciously curbs bile acid synthesis by inhibiting key enzymes involved in the biosynthetic pathway, both in cultured primary hepatocytes and in vivo. Contrarily, we observed that DMSO treatment did not confer protection against bile-acid-induced liver damage in two distinct mouse models: one induced by a 0.1% DDC diet, leading to bile duct obstruction, and another induced by a CDA-HFD, resulting in non-alcoholic steatohepatitis (NASH). Histopathological and biochemical analyses unveiled a comparable extent of liver injury and fibrosis levels in DMSO-treated mice, characterized by similar levels of increase in Col1a1 and Acta2 expression and equivalent total liver collagen levels. These results suggest that, while DMSO can promptly inhibit bile acid synthesis in healthy mice, compensatory mechanisms might rapidly override this effect, negating any protective impact against bile-acid-induced liver damage in mice. Through these findings, our study underscores the need to reconsider treating DMSO as a mere inert solvent and prompts further exploration to identify more effective therapeutic strategies for the prevention and treatment of bile-acid-associated liver diseases.
Correction: Krivonogov et al. Microbial Diversity and Authigenic Mineral Formation of Modern Bottom Sediments in the Littoral Zone of Lake Issyk-Kul, Kyrgyz Republic (Central Asia). Biology 2023, 12, 642
Authors: Krivonogov Sergei , Maltsev Anton , Zelenina Darya , Safonov Alexey
Published: August 2023

Sergei Krivonogov was not included as an author in the original publication [...]
Regular Sport Activity Is Able to Reduce the Level of Genomic Damage
Authors: Santovito Alfredo , Agostinovna Nigretti Angiolina , Sellitri Amedeo , Scarfò Manuel , Nota Alessandro
Published: August 2023

Regular physical activity is considered one of the most valid tools capable of reducing the risk of onset of many diseases in humans. However, it is known that intense physical activity can induce high levels of genomic damage, while moderate exercise can elicit a favorable adaptive response by the organism. We evaluated, by the buccal micronuclei assay, the frequencies of micronuclei, nuclear buds and binucleated cells in a sample of amateur athletes practicing different disciplines, comparing the obtained data with those of subjects who practiced sports just occasionally and subjects that did not practice sport at all. The aim was to evaluate whether physical activity affects background levels of genomic damage and whether the different sports disciplines, as well as some gene polymorphisms, differentially affect these levels. A total of 206 subjects, 125 athletes and 81 controls, were recruited. Athletes showed significantly lower values of micronuclei, nuclear buds and binucleated cells with respect to controls. Sprinters and Martial Artists displayed significantly higher frequencies of micronuclei than other categories of athletes. Finally, neither sex nor gene polymorphisms seemed to influence the levels of genomic damage, confirming that the observed genomic damage is probably due to the nature of the sport activity.
An Extracellular/Membrane-Bound S100P Pool Regulates Motility and Invasion of Human Extravillous Trophoblast Lines and Primary Cells
Authors: Lancaster Tara , Tabrizi Maral E. A. , Repici Mariaelena , Gupta Janesh , Gross Stephane R.
Published: August 2023

Whilst S100P has been shown to be a marker for carcinogenesis, we have shown, in non-physio-pathological states, that its expression promotes trophoblast motility and invasion but the mechanisms explaining these cellular processes are unknown. Here we identify the presence of S100P in the plasma membrane/cell surface of all trophoblast cells tested, whether lines, primary extravillous (EVT) cells, or section tissue samples using either biochemical purification of plasma membrane material, cell surface protein isolation through biotinylation, or microscopy analysis. Using extracellular loss of function studies, through addition of a specific S100P antibody, our work shows that inhibiting the cell surface/membrane-bound or extracellular S100P pools significantly reduces, but importantly only in part, both cell motility and cellular invasion in different trophoblastic cell lines, as well as primary EVTs. Interestingly, this loss in cellular motility/invasion did not result in changes to the overall actin organisation and focal adhesion complexes. These findings shed new light on at least two newly characterized pathways by which S100P promotes trophoblast cellular motility and invasion. One where cellular S100P levels involve the remodelling of focal adhesions whilst another, an extracellular pathway, appears to be focal adhesion independent. Both pathways could lead to the identification of novel targets that may explain why significant numbers of confirmed human pregnancies suffer complications through poor placental implantation.
Chronic Urticaria Biomarkers IL-6, ESR and CRP in Correlation with Disease Severity and Patient Quality of Life—A Pilot Study
Authors: Kuna Matea , Štefanović Mario , Ladika Davidović Blaženka , Mandušić Nikolina , Birkić Belanović Ines , Lugović-Mihić Liborija
Published: August 2023

(1) Background: To assess the relationship between serum interleukin-6 (IL-6), erythrocyte sedimentation rate (ESR) and C-reactive protein (CRP) values and disease severity in patients with chronic spontaneous urticaria (CSU) and to examine which of these serum biomarkers better indicates disease severity. (2) Methods: Our pilot study included 20 patients with CSU who filled out questionnaires concerning disease severity and quality of life (the Urticaria Activity Score summed over 7 days [UAS7], the once-daily Urticaria Activity Score [UAS], the Urticaria Control Test [UCT], and the Dermatology Life Quality Index [DLQI]). Blood samples were taken to measure IL-6, ESR and CRP. (3) Results: ESR significantly correlated with the UAS7 (linear and moderate correlation; r = 0.496; p = 0.026), while CRP did not correlate with disease severity. IL-6 correlated with the once-daily UAS (r = 0.472; p = 0.036) and DLQI (r = 0.504; p = 0.023) (linear and moderate correlation) but not the UAS7 or UCT. (4) Conclusions: IL-6 was a better indicator of the once-daily UAS and DLQI, while ESR was a better indicator of the UAS7 (there was no correlation between IL-6, CRP and ESR parameters). Although our results are promising, this study should be conducted with a larger number of CSU patients.
Drug Discovery and Development of miRNA-Based Nucleotide Drugs for Gastrointestinal Cancer
Authors: Sato Hiromichi , Hara Tomoaki , Meng Sikun , Tsuji Yoshiko , Arao Yasuko , Sasaki Kazuki , Miyoshi Norikatsu , Kobayashi Shogo , Doki Yuichiro , Eguchi Hidetoshi , Ishii Hideshi
Published: August 2023

Short non-coding RNAs, miRNAs, play roles in the control of cell growth and differentiation in cancer. Reportedly, the introduction of miRNAs could reduce the biologically malignant behavior of cancer cells, suggesting a possible use as therapeutic reagents. Given that the forced expression of several miRNAs, including miR-302, results in the cellular reprograming of human and mouse cells, which is similar to the effects of the transcription factors Oct4, Sox2, Klf4, and c-Myc, this suggests that the selective introduction of several miRNAs will be able to achieve anti-cancer effects at the epigenetic and metabolic levels. In this review article, we bring together the recent advances made in studies of microRNA-based therapeutic approaches to therapy-resistant cancers, especially in gastrointestinal organs.
Human Gm, Km, and Am Allotypes: WHO/IMGT Nomenclature and IMGT Unique Numbering for Immunoinformatics and Therapeutical Antibodies
Authors: Lefranc Marie-Paule , Lefranc Gérard
Published: August 2023

Human immunoglobulin allotypes are allelic antigenic determinants (or “markers”) determined serologically, classically by hemagglutination inhibition, on the human immunoglobulin (IG) or antibody heavy and light chains. The allotypes have been identified on the gamma1, gamma2, gamma3, and alpha2 heavy chains (designated as G1m, G2m, G3m, and A2m allotypes, respectively) and on the kappa light chain (Km allotypes). Gm and Am allotypes have been one of the most powerful tools in population genetics, as they are inherited in fixed combinations, or Gm–Am haplotypes, owing to the linkage of the human IGHC genes in the IGH locus on chromosome 14. They have been very instrumental in molecular characterization of the human IGHC genes (gene polymorphisms or alleles, and IG heavy-chain structure in domains) and of the IGH locus (IGHC gene order, gene conversion, and copy number variation (CNV)). They represent a major system for understanding immunogenicity of the polymorphic IG chains in relation to amino acid and conformational changes. The WHO/IMGT allotype nomenclature and the IMGT unique numbering for constant (C) domain bridge Gm–Am and Km alleles to IGHC and IGKC gene alleles and structures and, by definition, to IG chain immunogenicity, opening the way for immunoinformatics of personalized therapeutic antibodies and engineered variants.
How Social Identity Affects Green Food Purchase Intention: The Serial Mediation Effect of Green Perceived Value and Psychological Distance
Authors: Zheng Can , Ling Shuai , Cho Dongmin
Published: August 2023

As the global population continues to grow, the impact of environmental damage and resource depletion has been severely increased. In this context, green food gains tremendous potential as a sustainable solution. This study establishes a model framework around social identity, psychological distance, green perceived value, and purchase intention from the perspective of social identity to explore the impact the social group has on individual green food purchase intention. Data from 497 questionnaires collected in China were validated using SPSS26 and SmartPLS4. The results demonstrated that the model exhibited excellent explanatory power for psychological distance (R2 = 47.5%), green perceived value (R2 = 48.2%), and purchase intention of green food (R2 = 54.7%). Path analysis showed that social identity, psychological distance, and green perceived value significantly positively affected green food purchase intention. The results also show that social identity significantly positively affected psychological distance and green perceived value, while psychological distance has a significant positive influence on green perceived value. Additionally, it is concluded that psychological distance and green perceived value have significant mediating and serial mediating effects on social identity and green food purchase intention. These findings bridge the research gap concerning consumers’ green food purchase intention from a group perspective, thereby offering great insights for the formulation of sustainable policies. Furthermore, the study provides both theoretical and practical implications for the expansion of the green food consumption market.
Spatial Distribution and Typological Classification of Heritage Buildings in Southern China
Authors: Gao Han , Wang Yang , Zhang Hong’ou , Huang Jinyu , Yue Xiaoli , Chen Fan
Published: August 2023

Heritage buildings are a crucial aspect of a country’s cultural heritage, serving as a means of preserving and passing down its history and traditions to future generations. The heritage buildings in southern China possess significant conservation, utilization, and research value. However, research is lacking on the spatial distribution characteristics and subdivision types of these buildings in the region. This study aimed to investigate the spatial agglomeration and distribution characteristics of heritage buildings in southern China, as well as the factors contributing to the formation of these spatial distribution patterns. This article focused on the protection of 981 heritage buildings in southern China since the founding of China. The study examined the buildings’ spatial agglomeration and distribution characteristics from various dynasties and subdivided types. It utilized the average nearest neighbor analysis, unbalance index, and kernel density estimation to analyze this distribution. Additionally, this study also investigated the primary factors influencing the spatial distribution and differentiation of these buildings. The results demonstrated the following: (1) In general, the spatial distribution of heritage buildings in southern China is characterized by unevenness and clustering, with a concentration in the eastern coastal and Sichuan provinces. (2) In terms of temporal dimension, the spatial distribution of heritage buildings exhibits unique characteristics in various dynastic zones. (3) In the type dimension, the number of different types of heritage buildings varies greatly. (4) Further analysis of the distribution and types of heritage buildings indicates that quantitative differences are primarily influenced by natural, human, and socio-economic factors. This research was unique as it explored the geospatial distribution characteristics and determinants of heritage buildings. It offers a valuable perspective on the spatial distribution of heritage buildings and can serve as a reference for future studies on the preservation and protection of such buildings in China. Additionally, the findings can provide guidance for the management and rational use of heritage buildings in southern China.
Evaluating the Reactions of Bridge Foundations to Combined Wave–Flow Dynamics
Authors: Xiao Xian , Nie Jianwei
Published: August 2023

As the ongoing development of national infrastructure progresses, we see an increase in the construction of deep-water bridges, specifically cross-sea bridges. This paper uses Stokes’s wave theory to simulate and analyze how a bridge foundation dynamically responds to wave–fluid interactions. Firstly, the governing equations, boundary conditions and initial conditions of fluid motion are derived, expanded and solved via Stokes’s wave theory, and a spectral model is simulated and plotted. Based on the P-M spectrum and equal frequency method, a method of wave height attenuation during wave propagation is proposed. Using an SSTK-ω turbulence model, a numerical wave flume is established considering the fluid model, the selection of element type and the boundary conditions set, and the influencing factors of wave propagation (attenuation) are analyzed. Waves with different wave parameters (period, depth and height) are numerically simulated and compared with the theoretical values. Finally, we perform an analysis of the dynamic response under wave–current coupling conditions. We establish different operational scenarios and obtain the following results: under a load duration of 200 s, the peak transverse displacements for spans 1, 2 and 3 measure at 0.84 m, 0.63 m and 0.62 m, respectively. The peak transverse displacements under operational scenarios 2 and 3 show reductions of 25.0% and 25.7%, respectively, when compared to scenario 1. However, large transverse displacements remain. This suggests that the influence of waves and water flow on the transverse displacement of the main span should not be overlooked.
Review of Wind-Induced Effects Estimation through Nonlinear Analysis of Tall Buildings, High-Rise Structures, Flexible Bridges and Transmission Lines
Authors: Zhao Shuang , Zhang Chengtao , Dai Xianxing , Yan Zhitao
Published: August 2023

The nonlinear effects exhibited by structures under the action of wind loads have gradually stepped into the vision of wind-resistant researchers. By summarizing the prominent wind-induced nonlinear problems of four types of wind-sensitive structures, namely tall buildings, high-rise structures, flexible bridges, and transmission lines, the occurrence mechanism of their nonlinear effects is revealed, providing cutting-edge research progress in theoretical studies, experimental methods and vibration control. Aerodynamic admittance provides insights into the aerodynamic nonlinearity (AN) between the wind pressure spectrum and wind speed spectrum of tall building surfaces. The equivalent nonlinear equation method is used to solve nonlinear vibration equations with generalized van-der-Pol-type aerodynamic damping terms. The elastic–plastic finite element method and multiscale modeling method are widely employed to analyze the effects of geometric nonlinearity (GN) and material nonlinearity (MN) at local nodes on the wind-induced response of latticed tall structures. The AN in blunt sections of bridges arises from the amplitude dependence of the aerodynamic derivative and the higher-order term of the self-excited force. Volterra series aerodynamic models are more suitable for the nonlinear aerodynamic modeling of bridges than the polynomial models studied more in the past. The improved Lindstedt–Poincare perturbation method, which considers the strong GN in the response of ice-covered transmission lines, offers high accuracy. The complex numerical calculations and nonlinear analyses involved in wind-induced nonlinear effects continue to consume significant computational resources and time, especially for complex wind field conditions and flexible and variable structural forms. It is necessary to further develop analytical, modeling and identification tools to facilitate the modeling of nonlinear features in the future.
Strengthening of Reinforced Concrete Columns Using Ultra-High Performance Fiber-Reinforced Concrete Jacket
Authors: Shehab Hamdy , Eisa Ahmed , Wahba Ahmed Mohamed , Sabol Peter , Katunský Dušan
Published: August 2023

The use of Ultra-High Performance Fiber Reinforced Concrete (UHPFRC) increased in the field of civil engineering throughout the last few decades. UHPFRC is being used considerably on a large scale in megastructure applications. High compressive and tensile strength permits reconstruction and optimization of the structural members. At the same time, its improved durability properties make it easier to extend the life of the design and can be used as thin layers, cladding, repairs, and column coverings. Although UHPC has an extremely high compressive strength, it exhibits very brittle fracture behavior compared to normal strength concrete (NSC). Since the ductility and fracture toughness of UHPC can be enhanced by adding fibers, the addition of fibers to production adds innovative features to UHPFRC structures and opens up new application areas for UHPFRC. The aim of this study is to investigate the axial behavior of square reinforced concrete (RC) columns strengthened with UHPFRC jackets. Nineteen specimens were cast (1000 mm in height and a cross-sectional area of 150 × 150 mm, whose interface treatment methods were prepared through vertical grooving (VG), horizontal grooving (HG), and without grooving (NG), with the jacket thickness (20 mm and 40 mm) and the number of strengthened sides of the column (two, three, and four sides). The results show a brittle failure for all strengthened specimens. The UHPFRC-reinforced RC columns with vertical grooving (VG) showed a higher ultimate load capacity compared to the columns with horizontal grooving (HG) and the columns without grooving (NG). The horizontal grooving (HG) gives a better result than the jacket without grooving (NG) and increases the cohesion area between the jacket and the column for two and three of the RC columns’ strengthened sides. But, in the case of strengthening the columns on four sides, the effect of confining the jacket to the column appears, and the grooving causes weakness in the body of the original column so that the jacket without grooving (NG) gives a better result than the jacket with horizontal grooving (HG).
The Bone Microenvironment Soil in Prostate Cancer Metastasis: An miRNA Approach
Authors: Prigol Anne Natalie , Rode Michele Patrícia , da Luz Efe Fernanda , Saleh Najla Adel , Creczynski-Pasa Tânia Beatriz
Published: August 2023

Bone metastatic prostate cancer (PCa) is associated with a high risk of mortality. Changes in the expression pattern of miRNAs seem to be related to early aspects of prostate cancer, as well as its establishment and proliferation, including the necessary steps for metastasis. Here we compiled, for the first time, the important roles of miRNAs in the development, diagnosis, and treatment of bone metastasis, focusing on recent in vivo and in vitro studies. PCa exosomes are proven to promote metastasis-related events, such as osteoblast and osteoclast differentiation and proliferation. Aberrant miRNA expression in PCa may induce abnormal bone remodeling and support tumor development. Furthermore, miRNAs are capable of binding to multiple mRNA targets, a dynamic property that can be harnessed for the development of treatment tools, such as antagomiRs and miRNA mimics, which have emerged as promising candidates in PCa treatment. Finally, miRNAs may serve as noninvasive biomarkers, as they can be detected in tissue and bodily fluids, are highly stable, and show differential expression between nonmetastatic PCa and bone metastatic samples. Taken together, the findings underscore the importance of miRNA expression profiles and miRNA-based tools as rational technologies to increase the quality of life and longevity of patients.
Endobronchial Ultrasound/Transbronchial Needle Aspiration-Biopsy for Systematic Mediastinal lymph Node Staging of Non-Small Cell Lung Cancer in Patients Eligible for Surgery: A Prospective Multicenter Study
Authors: Divisi Duilio , Di Leonardo Gabriella , Venturino Massimiliano , Scarnecchia Elisa , Gonfiotti Alessandro , Viggiano Domenico , Lucchi Marco , Mastromarino Maria Giovanna , Bertani Alessandro , Crisci Roberto
Published: August 2023

Background: The treatment of lung cancer depends on histological and/or cytological evaluation of the mediastinal lymph nodes. Endobronchial ultrasound/transbronchial needle aspiration-biopsy (EBUS/TBNA-TBNB) is the only minimally invasive technique for a diagnostic exploration of the mediastinum. The aim of this study is to analyze the reliability of EBUS in the preoperative staging of non-small cell lung cancer (NSCLC). Methods: A prospective study was conducted from December 2019 to December 2022 on 217 NSCLC patients, who underwent preoperative mediastinal staging using EBUS/TBNA-TBNB according to the ACCP and ESTS guidelines. The following variables were analyzed in order to define the performance of the endoscopic technique—comparing the final staging of lung cancer after pulmonary resection with the operative histological findings: clinical characteristics, lymph nodes examined, number of samples, and likelihood ratio for positive and negative outcomes. Results: No morbidity or mortality was noted. All patients were discharged from hospital on day one. In 201 patients (92.6%), the preoperative staging using EBUS and the definitive staging deriving from the evaluation of the operative specimen after lung resection were the same; the same number of patients were detected in downstaging and upstaging (8 and 8, 7.4%). The sensitivity, specificity, positive and negative predictive value, and diagnostic accuracy were 90%, 90%, 82%, 94%, and 90%, respectively. The likelihood ratio for positive and negative results was 9 and 0.9, respectively, confirming cancer when present and excluding it when absent. Conclusions: EBUS is the only low-invasive and easy procedure for mediastinal staging. The possibility to check the method in each of its phases—through direct visualization of the vessels regardless of their location in relation to the lymph nodes—makes it safe both for the endoscopist and for the patient. Certainly, the cytologist/histologist and/or operator must have adequate expertise in order not to negatively affect the outcome of the method, although three procedures appear to reduce the impact of the individual professional involved on performance.
Plasma Clot Properties in Patients with Pancreatic Cancer
Authors: Thaler Johannes , Prager Gerald , Pabinger Ingrid , Ay Cihan
Published: August 2023

Pancreatic cancer is one of the most prothrombotic malignancies. Plasma clot properties may be altered in patients with pancreatic cancer, and circulating tissue factor (TF) may play an important role. We applied a modified plasma clot formation assay (only CaCl2 and phospholipids were added to initiate clotting) and a standard clotting assay (lipidated TF was also added) to investigate whether plasma clot properties are altered in pancreatic cancer patients (n = 40, 23 female) compared to sex-matched healthy controls. The modified assay was also performed in the presence of a TF blocking antibody. With this modified assay, we detected an increased plasma clot formation rate (Vmax) and an increased delta absorbance (ΔAbs, indicating fibrin fiber thickness) in patients compared to controls. These differences were not detected with the standard clotting assay. Following addition of a TF blocking antibody in in our modified assay, Vmax decreased significantly in patients only, ΔAbs significantly decreased in patients and in healthy controls, the lag phase did not change, and the time to peak fibrin generation increased in patients only. Taken together, these findings indicate the presence of a prothrombotic state in pancreatic cancer patients, which depends on TF and is detectable with our modified assay but not with a standard clotting assay.
A Systematic Review of Population-Based Studies of Chronic Bowel Symptoms in Cancer Survivors following Pelvic Radiotherapy
Authors: Biran Adam , Bolnykh Iakov , Rimmer Ben , Cunliffe Anthony , Durrant Lisa , Hancock John , Ludlow Helen , Pedley Ian , Rees Colin , Sharp Linda
Published: August 2023

Pelvic radiotherapy can damage surrounding tissue and organs, causing chronic conditions including bowel symptoms. We systematically identified quantitative, population-based studies of patient-reported bowel symptoms following pelvic radiotherapy to synthesize evidence of symptom type, prevalence, and severity. Medline, CINAHL, EMBASE, and PsychINFO were searched from inception to September 2022. Following independent screening of titles, abstracts, and full-texts, population and study characteristics and symptom findings were extracted, and narrative synthesis was conducted. In total, 45 papers (prostate, n = 39; gynecological, n = 6) reporting 19 datasets were included. Studies were methodologically heterogeneous. Most frequently assessed was bowel function (‘score’, 26 papers, ‘bother’, 19 papers). Also assessed was urgency, diarrhea, bleeding, incontinence, abdominal pain, painful hemorrhoids, rectal wetness, constipation, mucous discharge, frequency, and gas. Prevalence ranged from 1% (bleeding) to 59% (anal bleeding for >12 months at any time since start of treatment). In total, 10 papers compared radiotherapy with non-cancer comparators and 24 with non-radiotherapy cancer patient groups. Symptom prevalence/severity was greater/worse in radiotherapy groups and symptoms more common/worse post-radiotherapy than pre-diagnosis/treatment. Symptom prevalence varied between studies and symptoms. This review confirms that many people experience chronic bowel symptoms following pelvic radiotherapy. Greater methodological consistency, and investigation of less-well-studied survivor populations, could better inform the provision of services and support.
Parents, Teachers, and Community: A Team Approach to Developing Physical Competence in Children
Authors: Chapelski Matthew S. , Erlandson Marta C. , Stoddart Alexandra L. , Froehlich Chow Amanda , Baxter-Jones Adam D. G. , Humbert M. Louise
Published: August 2023

Increasing children’s physical activity engagement has short- and long-term health benefits. Developing physical competence is a key component of children’s engagement in physical activity. The purpose of our study was to assess if a 12-week home, school, and community-based physical literacy intervention improved the physical competence of children in kindergarten and grade one. Four schools were either assigned to receive the intervention (n = 2 schools) or continue with their usual practice (control sites) (n = 2 schools). Physical competence was evaluated pre- and post-intervention in 103 intervention (41 female) and 83 usual practice (36 female) children using PLAY Fun. PLAY Parent and PLAY Coach tools measured parent and teacher perspectives of children’s physical competence, respectively. The intervention effect was assessed with repeated measures MANOVA to evaluate change in physical competence, with alpha set at p < 0.05. Children in both groups improved their locomotor, object control, and overall physical competence (p < 0.05) over the 12-week intervention. There was a significant intervention effect for locomotor and overall physical competence (p < 0.05). Interestingly, parents did not perceive these changes in physical competence (p > 0.05). However, teachers perceived improved physical competence for children in the intervention. Our physical literacy intervention improved the physical competence of children in kindergarten and grade one.
Multiband Superconductivity in High-Pressure Sulfur Hydrides
Authors: Ummarino Giovanni Alberto , Bianconi Antonio
Published: August 2023

The temperature dependence of the two superconducting gaps in pressurised H3S at 155 GPa with a critical temperature of 203 K has been determined using a data analysis of the experimental curve of the upper critical magnetic field as a function of temperature in the framework of the two-band s-wave Eliashberg theory. Two different phonon-mediated intra-band Cooper pairing channels in a regime of moderate strong couplings have the key role of the pair-exchange interaction between the two gaps, giving the two non-diagonal terms of the coupling tensor, which are missing in the single-band s-wave Eliashberg theory. The results provide a prediction of the different temperature dependence of the small and large gaps as a function of temperature, which provides evidence of multigap superconductivity in H3S.
Involvement of Vitamin D3 in the Aging Process According to Sex
Authors: Trifan Daniela Florina , Tirla Adrian Gheorghe , Mos Calin , Danciu Adrian , Bodog Florian , Manole Felicia , Ghitea Timea Claudia
Published: August 2023

Background/Aim: Rapid onset of facial ptosis can impact physical appearance and compromise the outcomes of facelift procedures. The level of vitamin D has a potential correlation with collagen formation and its deficiency with inflammatory processes that affect the breakdown of hyaluronic acid. This study aims to investigate the potential relationship between accentuated facial ptosis in women and low levels of vitamin D. Furthermore, it aims to explore preventive measures or strategies to slow down facial ptosis and enhance the longevity of facelift results. Materials and Methods: The study was focused on monitoring the vitamin D levels in women and men with advanced facial ptosis and comparing them with a control group. Results: Notably, a direct association between gender and serum vitamin D levels was observed, indicating less sustainable outcomes in women. Conclusions: Women face additional challenges in the aging process due to hormonal shifts after menopause or premenopausal, which are associated with osteoporosis and lower vitamin D levels.
Nucleation of L12-Al3M (M = Sc, Er, Y, Zr) Nanophases in Aluminum Alloys: A First-Principles ThermodynamicsStudy
Authors: Liu Shuai , Liu Fangjun , Yan Zhanhao , Nie Baohua , Fan Touwen , Chen Dongchu , Song Yu
Published: August 2023

High-performance Sc-containing aluminum alloys are limited in their industrial application due to the high cost of Sc elements. Er, Zr, and Y elements are candidates for replacing Sc elements. Combined with the first-principles thermodynamic calculation and the classical nucleation theory, the nucleation of L12-Al3M (M = Sc, Er, Y, Zr) nanophases in dilutealuminum alloys were investigated to reveal their structural stability. The calculated results showed that the critical radius and nucleation energy of the L12-Al3M phases were as follows: Al3Er > Al3Y > Al3Sc > Al3Zr. The Al3Zr phase was the easiest to nucleate in thermodynamics, while the nucleation of the Al3Y and Al3Er phases were relatively difficult in thermodynamics. Various structures of Al3(Y, Zr) phases with the radius r < 1 nm can coexist in Al-Y-Zr alloys. At a precipitate’s radius of 1–10 nanometers, the core–shelled Al3Zr(Y) phase illustrated the highest nucleation energy, while the separated structure Al3Zr/Al3Y obtained the lowest one, and had thermodynamic advantages in the nucleation process. Moreover, the core–shelled Al3Zr(Y) phase obtained a higher nucleation energy than Al3Zr(Sc) and Al3Zr(Er). Core–doubleshelled Al3Zr/Er(Y) obtained a lower nucleation energy than that of Al3Zr(Y) due to the negative ΔGchem of Al3Er and the negative Al3Er/Al3Y interfacial energy, and was preferentially precipitated in thermodynamics stability.
Diagnostic and Therapeutic Approaches in Neurorehabilitation after Traumatic Brain Injury and Disorders of Consciousness
Authors: Lippert Julian , Guggisberg Adrian G.
Published: August 2023

Severe traumatic brain injury (TBI) may cause disorders of consciousness (DoC) in the form of coma, unresponsive wakefulness syndrome (UWS), or minimally conscious state (MCS). Despite significant advancements made over the last two decades in detecting, predicting, and promoting the recovery of consciousness in TBI patients with DoC, the available diagnostic and treatment choices remain limited. In cases of severe TBI, the dissolution of consciousness both in the acute and post-acute phases constitutes one of the major clinical findings and challenges. In clinical settings, neurologists and neurorehabilitation specialists are called on to discern the level of consciousness in patients who are unable to communicate, and to project outcomes and recommend approaches to treatment. Standards of care are not available to guide clinical decision-making for this population, often leading to inconsistent, inaccurate, and inappropriate care. Recent studies refer to network-based mechanisms of consciousness as a more promising method to predict outcomes and functional recovery. A further goal is the modulation of neural networks underlying awareness and arousal as the main components of consciousness. This review centers on the difficulties in characterizing individuals experiencing post-traumatic DoC and on the recent advancements made in the identification and prognostication of consciousness recovery through the utilization of advanced neuroimaging and electrophysiological techniques as well as biomarkers. Moreover, we discuss new treatment approaches and summarize recent therapeutic recommendations.
Radio-Guided Lung Surgery: A Feasible Approach for a Cancer Precision Medicine
Authors: Conte Miriam , De Feo Maria , Frantellizzi Viviana , Tomaciello Miriam , Marampon Francesco , Evangelista Laura , Filippi Luca , De Vincentis Giuseppe
Published: August 2023

Background: Radio-guided surgery is a reliable approach used for localizing ground-glass opacities, lung nodules, and metastatic lymph nodes. Lung nodules, lymph node metastatic involvement, and ground-glass opacities often represent a challenge for surgical management and clinical work-up. Methods: PubMed research was conducted from January 1997 to June 2023 using the keywords “radioguided surgery and lung cancer”. Results: Different studies were conducted with different tracers: technetium-99m-albumin macroaggregates, cyanoacrylate combined to technetium-99m-sulfur colloid, indium-111-pentetreotide, and fluorine-18-deoxyglucose. A study proposed naphthalocyanine radio-labeled with copper-64. Radio-guided surgery has been demonstrated to be a reliable approach in localizing a lesion, and has a low radiological burden for personnel exposure and low morbidity. The lack of necessity to conduct radio-guided surgery under fluoroscopy or echography makes this radio-guided surgery an easy way of performing precise surgical procedures. Conclusions: Radio-guided surgery is a feasible approach useful for the intraoperative localization of ground-glass opacities, lung nodules, and metastatic lymph nodes. It is a valid alternative to the existing approaches due to its low cost, associated low morbidity, the possibility to perform the procedure after several hours, the low radiation dose applied, and the small amount of time that is required to perform it.
The Effects of the Myobrace® System on Peripheral Blood Oxygen Saturation (SpO2) in Patients with Mixed Dentition with Oral Dysfunction
Authors: Levrini Luca , Persano Roberta , Piantanida Sofia , Carganico Andrea , Deppieri Alessandro , Naboni Giulia , Mastrapasqua Rodolfo Francesco , Saran Stefano
Published: August 2023

Introduction: Myobrace® is an orthodontic device that has the purpose of correcting oral dysfunctions, thus predisposing the physiological growth of the jaws, aligning teeth, and optimizing face development. This device is usually associated with Myobrace® Activities to reach this target. Considering the lack of studies in the literature about peripheral blood oxygen saturation (SpO2) and the use of preformed oral devices, the aim of this study is to quantify the change in blood oxygen saturation (SpO2) in patients treated with the Myobrace® System in mixed dentition. Materials and Methods: In this study, 23 children (11 females and 12 males) were involved, who were affected by different oral dysfunctions and were treated with a Myobrace®. Blood oxygen saturation measurements were taken at baseline and after every four months for a year. The SpO2 measurements were taken in the rest position and with a closed mouth for a total of 12 min—6 min with and 6 min without the Myobrace® oral device. All data points were anonymized and recorded on an Excel spreadsheet. A statistical analysis was carried out. Results: Therapy with a Myobrace® in patients with mixed dentition resulted in a statistically significant increase in oxygen saturation. In particular, in patients with a closed mouth, a statistically significant increase in oxygen saturation was observed, bringing it from 97.66% to 99.00%, while in the rest position, the increase was from 98.03% to 99.07%. Conclusions: The use of Myobrace® devices in patients with mixed dentition could lead to a significant improvement in blood oxygen saturation.
Analysis of Vibration Signals Based on Machine Learning for Crack Detection in a Low-Power Wind Turbine
Authors: Rangel-Rodriguez Angel H. , Granados-Lieberman David , Amezquita-Sanchez Juan P. , Bueno-Lopez Maximiliano , Valtierra-Rodriguez Martin
Published: August 2023

Currently, renewable energies, including wind energy, have been experiencing significant growth. Wind energy is transformed into electric energy through the use of wind turbines (WTs), which are located outdoors, making them susceptible to harsh weather conditions. These conditions can cause different types of damage to WTs, degrading their lifetime and efficiency, and, consequently, raising their operating costs. Therefore, condition monitoring and the detection of early damages are crucial. One of the failures that can occur in WTs is the occurrence of cracks in their blades. These cracks can lead to the further deterioration of the blade if they are not detected in time, resulting in increased repair costs. To effectively schedule maintenance, it is necessary not only to detect the presence of a crack, but also to assess its level of severity. This work studies the vibration signals caused by cracks in a WT blade, for which four conditions (healthy, light, intermediate, and severe cracks) are analyzed under three wind velocities. In general, as the proposed method is based on machine learning, the vibration signal analysis consists of three stages. Firstly, for feature extraction, statistical and harmonic indices are obtained; then, the one-way analysis of variance (ANOVA) is used for the feature selection stage; and, finally, the k-nearest neighbors algorithm is used for automatic classification. Neural networks, decision trees, and support vector machines are also used for comparison purposes. Promising results are obtained with an accuracy higher than 99.5%.
Adaptive Quantization Range Division Technique for Electronic Control Data Compression in CNC Machine Tools
Authors: Hu Weiqi , Zhou Huicheng , Yang Jianzhong , Hui Enming , Dai Chaoren
Published: August 2023

With the development of new technologies such as artificial intelligence and big data, Industry 4.0 in manufacturing has been launched. As the core pillar of industrial manufacturing, computer numerical control (CNC) machine tools face significant challenges in data acquisition transmission and storage due to their complex structure, high volume of data points, strong time-series characteristics, and large amounts of data. To address the shortcomings of existing compression algorithms in quantization methods for large amounts of data in the instruction-domain, this paper proposes a quantization method based on distortion rate evaluation and linear fitting entropy reduction transformation, which aims to compress state signals such as the load power and load current while ensuring the availability of the data. This approach provides technical support for the transmission of high-frequency big data and meets the lightweight data acquisition requirements of digital twins for CNC machine tools. Compared to the empirical approach, this approach was more accurate and more computationally efficient.