bluuebunny/crossref_metadata_2025 huggingface dataset:
Food Production and Consumption in Cameroon
Authors: Leypey Mathew Fomine Forka
Published: March 2024

This article presents a preliminary survey of the precolonial and contemporary evidence for the patterns of cultural and commercial relationships established through food production and consumption in multiple ethnic groups in Cameroon. The sampled groups have been carefully selected to form a north/south transect running the length of the country. From the shores of the Atlantic Ocean in the South West Region to the Lake Chad basin in the north, the groups are Bakweri, Mbo, Bamum, Wawa, Doowaayo, and Kotoko. The members of these ethnic groups exchanged (and continue to exchange) different food items in cultural and commercial spheres.
The Truth About Mrs Marshall
Authors: Jenkins Terry
Published: March 2024

We are all familiar today with the names of celebrity chefs. They can be so well-known that a first name is enough to identify them: two from Britian that imme­diately spring to mind are Nigella and Delia. Their surnames, Lawson and Smith, are unnecessary. In the Victorian era another celebrity cook was born with the surname Smith. She achieved fame, however, under her married name: Mrs Agnes Bertha Marshall, and was more famous in her day than Mrs Beeton. She published cookery books, lectured and gave public demonstrations on the subject. She edited a magazine called The Table and, with her husband, ran a cookery school in Mortimer Street, Westminster. She popularized the making of ice-cream with her patent inventions, and is credited with devising the first ice-cream cone for one of her recipes. But, as so often happens, her name and achievements faded from public memory after her death in 1905. This article augments what we know about Mrs. Marshall.
Ken Albala: Noodle Soup: Recipes, Techniques, Obsession
Authors: Jaine Tom
Published: March 2024

Ken Albala: Noodle Soup: Recipes, Techniques, Obsession: University of Illinois Press, 2018: 186 pp., paperback, £16.99.
MILO - a Conceptual Learning Model Grounded in a Hermeneutical and a Caritative Caring Perspective: Development and Evaluation
Authors:   ,   Linnaeus University, Faculty of Health and Life Sciences, Department of Health and Caring Sciences., Koldestam Maria
Published: March 2024

Aim: The overall aim was to develop and evaluate a conceptual learning model grounded in a caritative caring perspective aimed to facilitate undergraduate nursing students’ learning during clinical practice. Methods: This thesis comprises four studies with a descriptive explorative design overall. In the inductively applied research, different designs using both qualitative and quantitative methods, were used. Studies I–III used qualitative methods; data for study I were collected using the Delphi method and analysed using qualitative data analysis. In study II, data were collected using focus group interviews and analysed using latent content analysis. In study III, data were collected using individual interviews and analysed using a phenomenographic approach. Study IV used quantitative methods and data were collected using a questionnaire and analysed using statistical methods. Results: Study I resulted in a conceptual learning model grounded in hermeneutics and a caritative caring perspective. The Model for Improvement in Learning Outcomes (MILO) encompasses eight concepts: four intrapersonal, i.e. the students’ own characteristics, reflecting understanding, and four contextual concepts, i.e. environmental concepts, reflecting structure. Study II showed that students’ learning is facilitated as a result of natural actions and elements that occur in daily life, integrating natural caring with professional caring. Studies III and IV showed that students’ learning was a gain in knowledge and understanding of supportive elements for learning and the perspective of the patients, and a gain in engagement and dedication. Study IV also showed that the intrapersonal concepts were valued more than the contextual concepts in the three different semesters studied. The use of the applications was valued more at the start of the students’ education. Some of the concepts and their applications had not been used in accordance with MILO’s implementation in the region involved. Conclusions: The fundamentals needed to become a professional caring nurse include having compassion and competence. Undergraduate nursing students’ learning during clinical practice needs to be facilitated by a theoretical foundation establishing an ethical bearing, by knowledge and understanding of one self and of the patient as a whole, and by challenged learning using a diversity of tools to achieve the intended outcome of better health and well-being for the patient.
The Genesis of Personal Data: Informational and Legal Aspect
Authors:   Russian State University of Justice, Stebivko Alexey
Published: March 2024

Introduction. The paper reveals the historical aspect of the emergence and development of the legal protection of personal data in Russia. The prerequisites for the formation of a conscious need for such protection on a Russian basis are analyzed. The author expresses the idea of the origin of the legal protection of personal data in the depths of the right to privacy, since the latter was not only formed at the earlier stages of the historical development of society but also prepared the conditions and legal tools for the emergence of this institution in the future. Purpose. The origin of the legal institution of personal data protection in Russia is analyzed. Methods. The methodological framework for the research consists of the methods of system analysis, historico-legal, and comparative law. Results. The right to privacy in Russia is presented in the paper as divided into four historical stages. The first stage is associated with the wide expansion of writing in Russia, which necessitated the protection of information contained in postal correspondence. Also, at this stage, only a small number of personal rights were protected. At the second stage, as a result of Peter the Great’s reforms, fundamental changes took place in public life in Russia, which led to the further development of Russian legislation, including the right to privacy. The result of such changes, according to the author of the paper, should be recognized as the consolidation of a sufficiently large number of elements of the right to privacy in the Code of Laws of the Russian Empire. The next stage in the history of the establishment of the institution of personal data in this country is associated with Soviet law. It is characterized by the fact that Soviet law “did not remain aloof” from the rapid development of the world community’s interest in human rights and freedoms in the twentieth century. And finally, at the fourth (modern) stage, the concepts of “personal data” and “their legal protection” arise and exist as independent ones, forming a separate legal institution for the protection of personal data within the framework of private law. Conclusions. Analyzing the above-mentioned concepts and categories as a result of scientific, technical, and social progress, as well as society’s awareness of the need to protect the private sphere of human life, the author states that the legislator’s “attention” is increasingly shifting from state to private secrets, and even the mechanism of the legal provision of state secrets is becoming more and more similar to the mechanism of private law protection.
Prostitution and the Provision of Sexual Services: Topical Regulatory Issues in the World and in Russia
Authors:   Belgorod Law Institute of the Ministry of Internal Affairs of Russia named after I.D. Putilin, Arkhiptsev Ivan , Sarychev Alexander ,   Belgorod Law Institute of the Ministry of Internal Affairs of Russia named after I.D. Putilin, Kuzmin Kirill ,   Belgorod Law Institute of the Ministry of Internal Affairs of Russia named after I.D. Putilin
Published: March 2024

Introduction. In the modern world, the public understanding of sexual mania, sexual freedom, and erotic preferences is undergoing significant changes caused by the influence of telecommunications systems, the modernization of moral values, and a change in moral guidelines. The tolerance of prostitution and sexual deviations has a negative impact on the normal physical, mental, spiritual, and moral development of citizens, distorts the consciousness of society, and especially affects minors, which can have an extremely negative impact on their further social adaptation in society. These phenomena are one of the most pressing and acute problems of Russian society, since the deformation of the physical and mental development of civil society threatens the health of the nation. A particular danger is the moral corruption of children and adolescents, the formation of premature unhealthy sexual interest in them, the development of a misconception about sexual relationships, and unnatural norms of sexual life. In the paper, the authors examined the specifics of the regulation of prostitution in certain foreign countries and in Russia and proposed some measures to improve the legislation. The purpose of the research is to study the legal regulation of prostitution in foreign countries and in Russia, as well as to improve the current Russian legislation. Methods. The methodological framework for the study was the comparative legal method. The methods of induction and deduction used contributed to obtaining most of the formulated conclusions. Results. Based on the research conducted, some measures are proposed to tighten the current Russian legislation aimed at preventing prostitution. The authors’ definitions of the concepts of “prostitution” and “sexual services” are formulated. Conclusions. It is proposed to distinguish the concepts of “prostitution” and “sexual services,” taking as a basis the fundamental concept of service.
The Formation of Modern Approaches to Determining the Essence and Content of the Preliminary Investigation Stage
Authors:   Volgograd Institute of Management, a branch of the Russian Presidential Academy of National Economy and Public Administration, Kolosov Nikolay , Solovyova Natalia ,   Volgograd State University
Published: March 2024

Introduction. In numerous works of modern scientists dealing with the forms of preliminary investigation, despite the sufficient elaboration and interest in the topic under consideration, there is no uniform interpretation of the signs of this stage, which significantly complicates the law enforcement activities of law enforcement officers and may jeopardize the observance of the procedural rights and freedoms of the suspect (accused) and the victim. The purpose of the study was to develop conceptual proposals for improving the criminal procedure legislation in terms of regulating the preliminary investigation in the system of stages of the criminal process. To achieve this goal, modern approaches are considered, ranging from defining a preliminary investigation as a stage of preliminary proceedings to recognizing its exclusivity. Research objectives. To characterize the current positions of scientists, processualists, and practitioners regarding the definition of the essence and content of the preliminary investigation as a stage of the criminal process and its forms as content filling, and to identify promising areas for improving the domestic legislation, including taking into account the experience of the development of the criminal procedure legislation of the post-Soviet states. Methods. The methodological framework for the study consists of the general scientific methods (dialectical, systemic, structural-functional, logical, etc.) and the specific scientific methods (formal legal, comparative law, etc.). Results. The combined position on the essence of the preliminary investigation seems to be the most accurate and correct with regard to the general principles of judicial proceedings. The conclusion is made about the special position of the preliminary investigation as the main “source” of evidentiary information for further consideration of the case in court on its merits.
The Legal Consequences of the Transfer of the Lender’s Rights Under a Convertible Loan Agreement
Authors:   Arbitration Court of the Volga District, Ananyev Roman
Published: March 2024

Introduction. The paper analyzes the legal consequences of the transfer of the lender’s rights under a convertible loan agreement. Purpose. To consider four blocks of issues: the specifics of the legal regulation of the transfer of rights under a convertible loan agreement; the characteristic features of the assignment of a claim under a convertible loan agreement are determined; the features of the transfer of the lender’s rights under a convertible loan agreement are identified on the grounds provided for by law; and the convertible loan agreement is considered in connection with the implementation of the principle of stability of the corporation’s activities. Methods. The methodological framework for the research is based on the methods of scientific cognition, including such main ones as the methods of consistency, analysis, and comparative law. Results. The civil legislation of the Russian Federation mainly provides for two grounds for transferring the rights of a creditor to another person: making a corresponding transaction, called “assignment of a claim,” and the law (Paragraph 1 of Article 382 of the Civil Code of the Russian Federation). Conclusions. It is established that both grounds are equally relevant for a convertible loan agreement and require special scientific analysis.
Gas Distribution and Maintenance Services for Gas Equipment: Updating the Regulations Based on Centralization
Authors:   LLC “Gazprom Gazoraspredelenie Volgograd”, Puzikov Sergey
Published: March 2024

Introduction. In 2021, as part of the implementation of the initiative of Vladimir Putin, Russia’s president, amendments were made to the current legislation providing for the highest possible degree of gasification of the country, including the construction of gas networks to land plots of individuals without using the funds of these citizens, which was the beginning of reforms in the field of maintenance of gas equipment, taking into account the increase in gas consumers. In particular, the Government of the Russian Federation adopted a “roadmap” for the gasification of Russian regions, whereby the first stage should be completed in 2023 and the second stage was supposed to be implemented from 2023 to 2030. At the same time, the number of emergency incidents related to the use of gas, which have caused significant property damage, harm to health, and deaths, continues to remain at a critically high level. The analysis of the legal relations developing in the market of services under consideration indicates obvious shortcomings of the legal regulation. The purpose and objectives of the work are to: investigate the participation of a gas distribution organization in the maintenance of in-house gas equipment under the legislation amendments in this area; consider the features and problems of the legal regulation of maintenance activities; and propose recommendations for improving the legislation regarding the status of a gas distribution organization. Results. Currently, the legal status of gas distribution and gas equipment servicing organizations is unstable; intensive and contradictory legislative changes indicate that this type of activity needs to be updated in the legal regulations. The quality of the legal regulation of the activities of gas distribution and gas equipment servicing organizations operating in modern Russia requires deep improvement. Conclusions. A retrospective analysis of the legal regulation of the maintenance of in-house gas equipment allows for the formulation of a number of proposals; in particular, the state regulation of prices for in-house gas maintenance should be introduced. These maintenance services should be provided by specialized organizations without charging the public. The cost of services determined by the regulator must be included in the gas price and charged once by the supplier as part of the price under the gas supply agreement. In addition, the gas supplier must have a contractual relationship with a specialized organization that performs in-house gas maintenance. The population also needs timely diagnostics of gas equipment, its replacement, repair work, and proper maintenance and repair of house ventilation ducts so that gas supply is a means of comfortable living, which can be effectively carried out by Gazprom PJSC through its subsidiary specialized organization.
The Evolution of the Russian Constitution and the State-Civilization as a Conceptual Paradigm of the New Basic Law of Russia
Authors:   Volgograd Academy of the Ministry of Internal Affairs of the Russian Federation, Grachev Nicolay
Published: March 2024

Introduction. The relevance of the study. One of the key worldviews of the resurgent state ideology of modern Russia is the idea of a state-civilization, which acted as the doctrinal basis of the recently adopted Concept of Foreign Policy of the Russian Federation. In this regard, there is a need to describe the legal characteristics of the state-civilization and their adequate reflection in the constitutional legislation, which puts on the agenda the issue of developing and adopting a new Constitution of the Russian Federation that meets its civilizational characteristics and development goals. The purpose is to analyze the contradictions of the Constitution of the Russian Federation reformed in 2020, substantiate the idea of a state-civilization as the conceptual basis of the new Basic Law of the country, and argue for the need to start work on it in the near future. The methodological framework for the research is the civilizational approach in combination with the general scientific and special methods of cognition: analysis, synthesis, induction, deduction, technical, comparative law, and historical-legal. The scientific novelty of the study lies in the fact that, based on a civilizational approach, it outlines the tasks facing domestic legal science in connection with the official recognition of Russia as a civilizational state and identifies some conceptual ideas that, in the author’s opinion, could form the basis of the new Russian Constitution. Results and conclusions. The official recognition of Russia as a civilizational state is in fact an announcement of a course towards building an empire. Therefore, the main direction of constitutional reform at this historical stage is the legalization of the Russian government in this capacity. The main principles of the new Constitution of Russia, which allows for the consolidation of Russian society in its confrontation with the collective West, are the ideas of social justice and state sovereignty. It is in them that the request of Russian society to the state is revealed as fully as possible. All other political and legal principles and norms can be important, accepted by the public consciousness, and enshrined in the Constitution only if they correspond to these fundamental values.
The Concept and Content of the Right of Children to Be Under Care, or What Is the Parental Duty?
Authors:   Volgograd Institute of Management, Branch of the Russian Academy of National Economy and Public Administration, Usanova Victoria , Kayl Yanina ,   Volgograd Institute of Management, Branch of the Russian Academy of National Economy and Public Administration
Published: March 2024

Introduction. Despite all the evidence and, at first glance, the simplicity of caring for children (anyone can formulate their opinion about what it should include), its content is not understandable, clear, and the same for everyone. The study of the responsibility of parents to take care of children is highly relevant, since upbringing and caring for children are the main tasks of the parental role and are of great importance for the development and well-being of children. First of all, it is necessary to determine which specific types of care are an integral part of parental duty. This can cover aspects such as children’s physical health (nutrition, sleep, hygiene), emotional and psychological well-being (support, love, understanding), as well as education and development (study, socialization, skill development). The purpose of the study was to identify the problems of understanding child care and formulate the authors’ concept of care and its content. Methods. Induction, deduction, analysis, and formal legal methods were of crucial importance for obtaining the results. Results. Systematized the factors about which a parent should be constantly concerned about children; identified a set of actions that a parent should perform in connection with the care of minor children; defined the benefits for the child that form the content of care. The authors’ definition of child care is given. Conclusions. Taking care of children is a parent’s right and duty. The right and duty are independent elements of the legal status of an individual. The right and duty are an analogy to a positive legal responsibility as the encouragement for necessary behavior.
Independence in School Education and Scientific Work
Authors:   Polish Academy of Sciences, Okoń Wincenty
Published: March 2024

The paper discusses the importance of independence of thinking and acting, so in the school education as in the scientific work. In broader understanding independence means such an attitude of the intellect, emotions and will that makes one willingly undertake different tasks-problems and realize-solve them in a responsible way. Thus independence means the steady disposition to intellectual and practical activity in schools, universities and in the research work. When this activity is emotionally positive, it may create the basis for the development (in the process of school-university education) of scientific, social and moral convictions and attitudes, necessary not only for understanding the universe and for changing it, but also for the development of wisdom, which may be understood as harmony between to have and to be, among material needs and moral values.
A SLOTTED PENTAGON SHAPE QUADBAND TWO-ELEMENT MILLIMETER WAVE MULTIPLE-IN, MULTIPLE-OUT ANTENNA USING THEORY OF CHARACTERISTIC MODES
Authors: Parveez Shariff B.G. , Pathan Sameena , Mane Pallavi R. , Ali Tanweer
Published: March 2024

This article proposes the millimeter wave two-element multiple-in, multiple-out (MIMO) for wireless
applications. The designed antenna is closely packed with a dimension of 0.42 λ0 × 1.03 λ0. The antenna
resonates at quad band with good gain and impedance matching. The single-element structure is
a pentagon shape with two slits at the edges. Single-element structure performance is studied through
characteristic mode analysis. The MIMO antenna decoupling structure has been effective, resulting in
an isolation |S21| of 30 dB. The antenna has broadside radiation. The MIMO antenna also satisfies
the diversity performance of envelope correlation coefficient, diversity gain, channel capacity loss, total
active reflection coefficient, and mean effective gain.
Damned Lies
Authors: Waldron Jeremy
Published: March 2024

A popular saying distinguishes lies from damned lies: this paper asks about the difference. It does so as a way of considering the variety of lies told by former President Trump and the variety of ways in which his lying was wrong. Specifically it focuses on the manipulative aspect of lies, the grave and malicious damage that a lie may do to particular person, and the ways in which lying degrades and undermines conventions associated with human communication in various specific areas of discourse. Some of these ideas are found in Kant's moral philosophy, and Kant's views on lying are examined sympathetically and in detail. Many of President Trump's lies were banal and self-serving. But they underline our sense of a common world and the importance of truth as a touchstone in politics as in everyday life. Trump's lies about the 2020 election are given special attention in light of the point that his duty was not so much to convey accurate information--we have other sources for that--but to perform the formal task of *affirming* the results in order to allow the political system to continue operating.
Experiencias prospectivas al sur de la Argentina desde una institución pública de ciencia y tecnología (2019-2023)
Authors: González Leticia , Menni María Fernanda
Published: March 2024

A través de la reflexión sobre dos experiencias prospectivas conducidas por las autoras, este trabajo da cuenta de los interrogantes y particularidades que surgen al encarar este tipo de ejercicios en un organismo público de Ciencia y Tecnología en Argentina:  el Instituto Nacional de Tecnología Agropecuaria (INTA). Las experiencias fueron llevadas adelante en el Alto Valle del río Negro (Provincias de Neuquén y Río Negro) y el sudoeste de la Provincia de Buenos Aires entre los años 2019 y 2023. Partiendo de concebir a la prospectiva como una disciplina que busca alojar la complejidad y generar miradas compartidas sobre el futuro, lo que interesa aquí no es resaltar los resultados de los ejercicios, sino centrarnos en algunas características distintivas del proceso. Se concluye que, si bien no existe una respuesta única y unívoca a la pregunta planteada, los trabajos llevados adelante desde la institución permiten construir una “caja de herramientas” disponible cuando se enfrenta la necesidad de realizar este tipo de estudios.
Systemic steroids in pediatric pseudotumor cerebri - A case report
Authors: Shruthi K
Published: March 2024

Study design: Case report. Purpose: To study the efficacy of systemic steroids in pediatric pseudotumor cerebri. Introduction: Pseudotumor cerebri is a condition caused by elevated intracranial pressure presenting most commonly with headache. It is a diagnosis of exclusion. Methods: A 15-year-old girl presented in our outpatient department with a headache for 3 months, more in the posterior aspect, continuous type with severe intensity, non-radiating. On examination, the best corrected visual acuity in her right eye was 6/9, and her left eye was 6/6 parts. She had a normal anterior segment and normal Intraocular Pressure (IOP). Fundoscopy showed both optic discs edematous, pink in color with blurry disc margins, tortuous disc vessels, and edematous peripapillary area. The rest of the retina was normal. Lumbar puncture showed elevated opening CSF pressure and normal CSF composition. Hence she was diagnosed with IIH. She was started on oral prednisolone 1mg/kgbw once a day dosage, oral acetazolamide 250 mg once a day, topical nepafenac 0.1% one drop thrice a day and timolol 0.5% eye drops twice a day. Discussion: The main goals of treatment are alleviation of symptoms, including headache, and preservation of vision. Steroids were commonly used for treating IIH in the past, but cause significant long-term side effects, such as weight gain, that are undesirable in IIH patients. Furthermore, withdrawal of steroids can cause rebound intracranial hypertension. Thus, steroids should not be used routinely for IIH treatment. Results: By the end of one month, the patient was relieved of symptoms, best corrected visual acuity was 6/6 in both eyes with normal IOP, normal optic disc in both eyes. Conclusion: Steroids are an effective way of managing idiopathic intracranial hypertension in pediatric patients.
Anti-GFP pull-down with syringe filter partition v1
Authors: zhao Dorothy
Published: March 2024

Aggregation of proteins containing expanded polyglutamine (polyQ) repeats is the cytopathologic hallmark of a group of dominantly inherited neurodegenerative diseases, including Huntington's disease (HD). Huntingtin (Htt), the diseaseprotein of HD, forms amyloid-like fibrils by liquid-to-solid phase transition. Macroautophagy has been proposed to clear polyQ aggregates, but the efficiency of aggrephagy is limited. Here, we used cryo-electron tomography to visualize the interactions of autophagosomes with polyQ aggregates in cultured cells in situ. We found that an amorphous aggregate phase exists next to the radially organized polyQ fibrils. Autophagosomes preferentially engulfed this amorphous material, mediated by interactions between the autophagy receptor p62/SQSTM1 and the non-fibrillar aggregate surface. In contrast, amyloid fibrils excluded p62 and evaded clearance, resulting in trapping of autophagic structures. These results suggest that the limited efficiency of autophagy in clearing polyQ aggregates is due to the inability of autophagosomes to interact productively with the non-deformable, fibrillar disease aggregates.
Bioinformatics Protocol for Investigating Novel Biomarkers, Conducting Statistical Analysis, and Exploring Molecular Pathways: A Unified Approach for Alzheimer's Disease v1
Authors: Satyanarayan Bhat Shreya , Vishal Adarsh , Anil Bandikatte Spoorthi , Niranjan Vidya
Published: March 2024

This protocol paper presents a comprehensive bioinformatics approach aimed at identifying novel biomarkers associated with Alzheimer's disease (AD) using RNA sequencing (RNA-seq) datasets obtained from the Gene Expression Omnibus (GEO) database. Alzheimer's disease is a complex neurodegenerative disorder characterized by progressive cognitive decline, and identifying biomarkers is crucial for early diagnosis and therapeutic intervention. Leveraging bioinformatics tools and methods, this protocol outlines a step-by-step procedure for analyzing RNA-seq data to uncover potential biomarkers. The protocol includes data collection from the GEO database, quality control assessment, differential expression analysis, functional annotation, pathway enrichment analysis, and molecular pathway identification. Statistical analysis is applied throughout the protocol to ensure the robustness and reliability of the results. By following this protocol, researchers can systematically identify and validate novel biomarkers associated with Alzheimer's disease, ultimately contributing to a better understanding of its underlying molecular mechanisms and facilitating the development of targeted therapeutic interventions.
HuBMAP | Digestion and scRNA Analysis of Skin v1
Authors: Karunamurthy Arivarasan , Park HJ
Published: March 2024

This method details digestion and scRNA analysis of the HuBMAP tissue skin specimens.
Unveiling Prolonged COVID Variants: A Protocol for Clustering and Phylogenetic Analysis v1
Authors: R Kulkarni Spoorthi , C Lavanya , Garai Baishali , Niranjan Vidya
Published: March 2024

Prolonged COVID-19 has emerged as a significant concern globally, with a subset of individuals experiencing persistent symptoms long after the acute phase of the infection. Understanding the genomic basis of prolonged COVID-19 can provide crucial insights into its pathophysiology and aid in the development of targeted therapeutic strategies. In this study, we retrieved genome sequences of COVID-19 from the National Center for Biotechnology Information (NCBI) database and employed a comprehensive pipeline to identify single nucleotide polymorphisms (SNPs). The primary objective was to investigate the clustering similarity of different variants of COVID-19 prevalent in the Indian population. By focusing solely on Indian population genome sequences, we aimed to capture the unique genetic landscape of COVID-19 variants circulating in this demographic. Our analysis revealed distinct SNP patterns across the Indian population, indicative of genetic diversity within the viral strains. Furthermore, we plan to map these identified SNPs to relevant pathways to elucidate their potential functional significance in the context of prolonged COVID-19.
Cryo-ET data acquisition, tomogram reconstruction, and analysis v1
Authors: zhao Dorothy
Published: March 2024

Aggregation of proteins containing expanded polyglutamine (polyQ) repeats is the cytopathologic hallmark of a group of dominantly inherited neurodegenerative diseases, including Huntington's disease (HD). Huntingtin (Htt), the disease protein of HD, forms amyloid-like fibrils by liquid-to-solid phase transition. Macroautophagy has been proposed to clear polyQ aggregates, but the efficiency of aggrephagy is limited. Here, we used cryo-electron tomography to visualize the interactions of autophagosomes with polyQ aggregates in cultured cells in situ. We found that an amorphous aggregate phase exists next to the radially organized polyQ fibrils. Autophagosomes preferentially engulfed this amorphous material, mediated by interactions between the autophagy receptor p62/SQSTM1 and the non-fibrillar aggregate surface. In contrast, amyloid fibrils excludedp62 and evaded clearance, resulting in trapping of autophagic structures. These results suggest that the limited efficiency of autophagy in clearing polyQ aggregates is due to the inability of autophagosomes to interact productively with the non-deformable, fibrillar disease aggregates.
0.1xBWT buffer v1
Authors: Schmidt Anna , Nagel Sarah , Meyer Matthias
Published: March 2024

Protocol for the preparation of 0.1x BWT buffer (Wash buffer II) for automated single-stranded DNA library preparation using the ssDNA2.0 method (Gansauge et al. 2020). References Gansauge, M.-T., Aximu-Petri, A., Nagel, S., & Meyer, M. (2020). Manual and automated preparation of single-stranded DNA libraries for the sequencing of DNA from ancient biological remains and other sources of highly degraded DNA. Nature Protocols, 15, 2279-2300.
Electrolyte Leakage Assay to Analyze Membrane Integrity in Leaves v1
Authors: Tovar-Rosales Laura , Arthikala Manoj-Kumar , Nanjareddy Kalpana
Published: March 2024

The production of reactive oxygen species occurs naturally in certain cellular organelles as metabolic byproducts. However, under stress conditions, their accumulation increases, resulting in cell death and the leakage of intracellular electrolytes. Consequently, quantifying electrolyte leakage has been widely accepted as an indicator of membrane integrity. This protocol delineates specific steps for measuring electrolyte leakage from plant tissues, providing insights into cellular damage caused by various stressors, including biotic, abiotic, and other factors.
Operant Conditioning v1
Authors: daniel daniel.dautan , Svenningsson Per
Published: March 2024

This protocol is for training mice operant conditioning to assess cognitive performance using the resources described in Matikainen-Ankney BA et al 2021.
Stringency wash buffer v1
Authors: Schmidt Anna , Nagel Sarah , Meyer Matthias
Published: March 2024

Protocol for the preparation of Stringency wash buffer for automated single-stranded DNA library preparation using the ssDNA2.0 method (Gansauge et al. 2020). References Gansauge, M.-T., Aximu-Petri, A., Nagel, S., & Meyer, M. (2020). Manual and automated preparation of single-stranded DNA libraries for the sequencing of DNA from ancient biological remains and other sources of highly degraded DNA. Nature Protocols, 15, 2279-2300.
Procedure for EEG surgery v2
Authors: daniel daniel.dautan , Svenningsson Per
Published: March 2024

Standard Operating Procedure for EEG surgery
Isolation of mCherry-LC3B and 97Q-GFP vesicles for LC-MS/MS v1
Authors: zhao Dorothy
Published: March 2024

Aggregation of proteins containing expanded polyglutamine (polyQ) repeats is the cytopathologic hallmark of a group of dominantly inherited neurodegenerative diseases, including Huntington's disease (HD). Huntingtin (Htt), the disease protein of HD, forms amyloid-like fibrils by liquid-to-solid phase transition. Macroautophagy has been proposed to clear polyQ aggregates, but the efficiency of aggrephagy is limited. Here, we used cryo-electron tomography to visualize the interactions of autophagosomes with polyQ aggregates in cultured cells in situ. We found that an amorphous aggregate phase exists next to the radially organized polyQ fibrils. Autophagosomes preferentially engulfed this amorphous material, mediated by interactions between the autophagy receptor p62/SQSTM1 and the non-fibrillar aggregate surface. In contrast, amyloid fibrils excluded p62 and evaded clearance, resulting in trapping of autophagic structures. These results suggest that the limited efficiency of autophagy in clearing polyQ aggregates is due to the inability of autophagosomes to interact productively with the non-deformable, fibrillar disease aggregates.
Gut-PFF Surgery v1
Authors: daniel daniel.dautan , Svenningsson Per
Published: March 2024

Methods for injection of alpha-synuclein preformed fibrils in the gut in a mice.
WHICH FRAME FOR WHAT GROUP? UNDERSTANDING DIFFERENTIAL FRAME RESONANCE THROUGH STANDPOINT: A CASE STUDY OF THE VEGAN MOVEMENT
Authors: Brockett Victoria
Published: March 2024

Innovative methods are enhancing our ability to measure the impact of framing on public perception, yet our understanding of the factors influencing frame resonance remains incomplete. This study investigates the differential effects of frames on diverse groups, proposing that both social positioning and the cultural context, alongside the qualities of the frames themselves, are significant. Drawing upon framing theory and feminist standpoint theories, I examine the vegan movement to understand the immediate impact of frames and social locations on their resonance. I employ an intersectional approach to analyze varying responses across gendered racial demographics, especially in response to radical narratives, using data from a new national survey of 498 United States participants. The findings show that individuals facing multiple forms of marginalization are more inclined to respond to frames that challenge existing power structures than those with multiple privileges. This suggests the critical role of standpoint in evaluating the validity of claims, highlighting the complex ways in which frames resonate across different social locations.
Phytotoxicity and genotoxicity assessment of organic and inorganic contaminants detected in pharmaceutical industrial wastewaters using Vigna radiata and Allium cepa
Authors: Jyoti Km , Soni Kuldeep , Chandra Ram
Published: March 2024

The discharged effluent of pharmaceutical industrial wastewater treatment plants (PIWWTPs) exhibits substantial environmental toxicity due to the intricate combination of organic and inorganic pollutants. This study assessed the phytotoxicity, genotoxicity, and cytotoxicity of untreated and treated pharmaceutical industrial wastewater (PIWW). Most of the physicochemical parameters viz. COD, BOD, EC, sulfide, sulfate, nitrate, phosphate, grease, phenols, and metal concentrations viz. B, Cr, Ca, Cd, Cu, Zn, Pb, Hg, and As in untreated wastewater (UTW) were noted beyond the permissible limit and remained higher in treated wastewater (TW). The findings revealed that the performance of PIWWTP was woefully inadequate. The GC-MS spectra of UTW and TW revealed the presence of various organic contaminants. The toxicological studies showed that the UTW had a high degree of phytotoxicity, which persisted even after the treatment as it inhibited the seed germination in Vigna radiata. The seed germination was inhibited up to 70% and 50% tested at 50% concentration of UTW and TW respectively. Genotoxicity was measured by determining mitotic index and chromosomal aberrations in Allium cepa root apex grown in untreated and treated PIWW. Compared to the negative control, the mitotic index dropped to 85% and 75% at the 50% concentrations of UTW and TW, respectively. Chromosomal aberrations were also found in the cellular mass of root apex growing in both UTW and TW. According to the findings, it is unsafe for the environment to release PIWW that has not been properly treated, as this could pose serious risks to environmental health.
The Influence of Sharia Commercial Bank Financing on Profitability
Authors: Indrayanti Shania Pasha , Mranani Muji , Dewi Veni Soraya
Published: March 2024

Introduction - The theme of this research is the effect of financing on the profitability of Islamic Commercial Banks. Profitability is the company's ability to generate profits with all the capital owned by the company. Companies or banks that have a high rate of return on investment use relatively small debt because the high rate of return allows the company to finance most of its internal funding.Objective – This research was conducted with the aim of analyzing the effect of mudharabah, musyarakah, murabahah, istishna'dan ijarah financing on profitability in Islamic commercial banks registered with OJK for the 2017-2021 period Methodology - This research uses quantitative research. The data used is secondary data obtained from the annual financial reports of Islamic commercial banks registered with the Financial Services Authority (OJK) for the 2017-2021 period. The number of samples used was 28 samples obtained using purposive sampling method. The analysis technique used is multiple linear regression with the help of the SPSS 25 program. Findings - The research results obtained show that the financing variables mudharabah, murabaha and ijarah have no effect on profitability, while musyarakah and istishna' variables have a negative effect on profitability. Implications - The results of this study are expected to improve banking performance from financial and non-financial aspects.
A Theoretical Book on Quality Assurance
Authors: Ojha Dr. Abhijeet , Dhaundhiyal Ms. Karuna , Dharmshaktu Mrs. Sarita Rawat , Singh Dr. Arun Kumar
Published: March 2024

The book “A Theoretical Book on Quality Assurance” is designed especially for 6th Semester B.Pharma students as per PCI course regulations. It reflects a comprehensive description and an overview of the existing knowledge of quality assurance making it appropriate for institutional purpose. This book consists of all the five units from Pharmaceutical Quality Assurance. Unit 1 deals with Quality Assurance and Quality Management concepts, Total Quality Management ICH guidelines. Unit 2 deals with Organization and Personnel, Premises, Equipment and Raw Material. Unit 3 deals with Quality Control and Good Laboratory Practices. Unit 4 deals with Document maintenance in Pharmaceutical industry. Unit 5 deals with Calibration and Validation. At the end of each unit examination oriented questions in the form of short answer type questions, long answer type questions and multiple choice questions are prepared.
Novel Drug Delivery System (NDDS)
Authors: Singh Mr. Prashant Kumar , Bhasin Mr. Rohit , Kumar Mr. Aditya , Rajan Mr. Nitin
Published: March 2024

"Novel Drug Delivery System (NDDS)" is an indispensable guide meticulously crafted by a team of dedicated authors—Mr. Prashant Kumar Singh, Mr. Rohit Bhasin, Mr. Aditya Kumar, and Mr. Nitin Rajan. Rooted in the latest PCI regulations for Bachelor of Pharmacy students, this comprehensive work is designed to navigate readers through the dynamic landscape of pharmaceutical sciences, with a particular focus on advancements in drug delivery systems.

Key Features:

Compliance with Latest PCI Regulations: Aligned with the most recent PCI guidelines, this book ensures that it meets the academic requirements for Bachelor of Pharmacy programs. Students and educators can trust its accuracy and relevance in the context of contemporary pharmaceutical education.
Holistic Coverage: "NDDS" provides a holistic exploration of novel drug delivery systems, covering a broad spectrum of topics, from fundamental principles to cutting-edge advancements. The content seamlessly integrates theoretical concepts with practical applications, offering a well-rounded understanding of the subject.
Collaborative Expertise: With each author contributing a unique perspective and specialized knowledge, the book benefits from a rich tapestry of insights. The collaboration reflects a shared commitment to enhancing the educational experience for students and preparing them for the challenges of the pharmaceutical industry.
Bridge Between Theory and Practice: Recognizing the importance of translating theoretical knowledge into practical skills, the book serves as a bridge between academia and real-world applications. It encourages critical thinking and fosters a mindset of innovation among aspiring pharmacists.
Inspiration for Future Innovators: Beyond meeting academic requirements, "NDDS" aims to inspire a new generation of pharmacists to actively engage with the evolving field of drug delivery systems. The authors hope to cultivate a spirit of curiosity and innovation that will contribute to the ongoing progress of pharmaceutical sciences.

Conclusion:
"Novel Drug Delivery System (NDDS)" is more than just a textbook; it is a collaborative effort by passionate authors who seek to elevate the standard of pharmaceutical education. By offering a comprehensive and PCI-compliant resource, the book is poised to become an essential companion for Bachelor of Pharmacy students, educators, and professionals alike. As the pharmaceutical landscape continues to evolve, this guide provides a solid foundation for understanding and navigating the intricacies of novel drug delivery systems.
Considering the assessment landscape in 2024
Authors: Darr Charles , Cosslett Graeme
Published: March 2024

With the new National-led government now in office, we can expect changes to assessment policy for schools. Given the critical role assessment plays in schooling, it is important that any proposed policy shifts are carefully scrutinised. In this Assessment News column, we identify some possible proposals the new government may be considering and make some brief comments on each one. We conclude with some recommendations.
ZIENTZIA, TEKNOLOGIA ETA BERRIKUNTZAKO EUSKADI 2030 PLANAREN EKARPENA GJH-EI
Authors: Izulain Ane , Edurne Edurne ,   Universidad de Deusto
Published: March 2024

Dokumentuaren helburua Zientzia, Teknologia eta Berrikuntzarako Euskadi 2030 Planak (ZTBP Euskadi 2030) Garapen Jasangarrirako Helburuei (GJH) egiten dien ekarpena neurtzea da.Horretarako, artearen egoeraren azterketa bat egiten da, I+G+b-k GJHei egiten dien ekarpenaren neurketari dagokionez, eta, horretan oinarrituta, txostenak neurketaproposamen metodologiko bat eta planak ZTBPn jasotako GJHei egiten dien ekarpenaren egungo mailaren lehen diagnostiko bat aurkezten ditu, erronka sozialen arabera antolatuta. Zehazki, honako hauek dira txostena egituratzen duten erronka sozialak eta erlazionatutako GJHak: Erronka soziala: osasuna (3. GJHa. Osasuna eta ongizatea). Erronka soziala: genero-berdintasuna (5. GJHa. Genero-berdintasuna). Gizarte-erronka: energia eta klima-aldaketa (7. GJH. Energia eskuragarria eta ezkutsatzailea. 11. GJHa. Hiri eta komunitate jasangarriak. 13. GJH. Klimaren aldeko ekintza). Erronka soziala: kalitatezko enplegua (8. GJHa. Lan duina eta hazkunde ekonomikoa). Erronka soziala: eraldaketa digitala (9. GJHa. Industria, berrikuntza eta azpiegiturak).
The contribution of the Euskadi 2030 Science, Technology and Innovation Plan to the SDGs
Authors: Izulain Ane ,   Universidad de Deusto, Magro Edurne
Published: March 2024

The aim of the document is to measure the contribution of the Euskadi 2030 Science, Technology, and Innovation Plan (STIP Euskadi 2030) to the Sustainable Development Goals (SDGs). To this end, an analysis is made of the state of the art regarding the measurement of the contribution of R&D&I to the SDGs, and on this basis, the report presents a methodological proposal for measurement and a first diagnosis of the current level of contribution of the plan to the SDGs included in the STIP 2030, ordered by social challenges. Specifically, the social challenges and related SDGs that structure the report are as follows: Social challenge: health (SDG 3. Health and wellbeing). Social challenge: gender equality (SDG 5. Gender equality). Social challenge: energy and climate change (SDG 7. Affordable and clean energy. SDG 11. Sustainable cities and communities. SDG 13. Climate action). Social challenge: quality employment (SDG 8. Decent work and economic growth). Social challenge: digital transformation (SDG 9. Industry, innovation and infrastructure).