bluuebunny/crossref_metadata_2025 huggingface dataset:
Mathematical Simulation of the Influence of Acoustic on the Efficiency of PM 2.5 Coagulation
Authors: Khmelev Vladimir , Shalunov Andrey , Golykh Roman
Published: February 2024

The particles of micron and submicron sizes (PM 2.5 and less) in gas environments pose a significant danger to humanity due to the emergence of specific and very dangerous diseases of the cardiovascular, respiratory, and immune systems of the human body. Such particles are the most difficult to detect; therefore, their effects on human health have only been discovered in recent decades. Classical ultrasonic coagulation by sinusoidal action turns out to be ineffective for PM 2.5 due to the peculiarities of the physical mechanisms of hydrodynamic and orthokinetic interaction realized in gaseous media. This article presents a theoretical justification for choosing ways to increase the efficiency of ultrasonic coagulation of PM 2.5 by creating special conditions under which nonlinear disturbances of the velocity and pressure of the gas phase in the ultrasonic field occur. The authors performed simulations of ultrasonic coagulation under nonlinear disturbances of the velocity (vortex) and the pressure (shock waves), which has numerical difficulties due to the instability of existing methods. As a result of the numerical analysis, the possibility of increasing the coagulation rate of particles in the submicron size range up to limit values (13 times due to nonlinear pressure disturbances, and an additional increase of at least 2 times due to aerosol compaction in the vortex field of gas velocity) was shown.
The Nearest Zero Eigenvector of a Weakly Symmetric Tensor from a Given Point
Authors: Pearson Kelly , Zhang Tan
Published: February 2024

We begin with a degree m real homogeneous polynomial in n indeterminants and bound the distance from a given n-dimensional real vector to the real vanishing of the homogeneous polynomial. We then apply these bounds to the real homogeneous polynomial associated with a nonzero m-order n-dimensional weakly symmetric tensor which has zero as an eigenvalue. We provide “nested spheres” conditions to bound the distance from a given n-dimensional real vector to the nearest zero eigenvector.
Tensor-Based Sparse Representation for Hyperspectral Image Reconstruction Using RGB Inputs
Authors: Duan Yingtao , Wang Nan , Zhang Yifan , Song Chao
Published: February 2024

Hyperspectral image (HSI) reconstruction from RGB input has drawn much attention recently and plays a crucial role in further vision tasks. However, current sparse coding algorithms often take each single pixel as the basic processing unit during the reconstruction process, which ignores the strong similarity and relation between adjacent pixels within an image or scene, leading to an inadequate learning of spectral and spatial features in the target hyperspectral domain. In this paper, a novel tensor-based sparse coding method is proposed to integrate both spectral and spatial information represented in tensor forms, which is capable of taking all the neighboring pixels into account during the spectral super-resolution (SSR) process without breaking the semantic structures, thus improving the accuracy of the final results. Specifically, the proposed method recovers the unknown HSI signals using sparse coding on the learned dictionary pairs. Firstly, the spatial information of pixels is used to constrain the sparse reconstruction process, which effectively improves the spectral reconstruction accuracy of pixels. In addition, the traditional two-dimensional dictionary learning is further extended to the tensor domain, by which the structure of inputs can be processed in a more flexible way, thus enhancing the spatial contextual relations. To this end, a rudimentary HSI estimation acquired in the sparse reconstruction stage is further enhanced by introducing the regression method, aiming to eliminate the spectral distortion to some extent. Abundant experiments are conducted on two public datasets, indicating the considerable availability of the proposed framework.
Derivation of Three-Derivative Two-Step Runge–Kutta Methods
Authors: Qin Xueyu , Yu Jian , Yan Chao
Published: February 2024

In this paper, we develop explicit three-derivative two-step Runge–Kutta (ThDTSRK) schemes, and propose a simpler general form of the order accuracy conditions (p≤7) by Albrecht’s approach, compared to the order conditions in terms of rooted trees. The parameters of the general high-order ThDTSRK methods are determined by utilizing the order conditions. We establish a theory for the A-stability property of ThDTSRK methods and identify optimal stability coefficients. Moreover, ThDTSRK methods can achieve the intended order of convergence using fewer stages than other schemes, making them cost-effective for solving the ordinary differential equations.
Fuzzy Divergence Measure Based on Technique for Order of Preference by Similarity to Ideal Solution Method for Staff Performance Appraisal
Authors: Saidin Mohamad Shahiir , Lee Lai Soon , Seow Hsin-Vonn , Pickl Stefan
Published: February 2024

Fuzzy set theory has extensively employed various divergence measure methods to quantify distinctions between two elements. The primary objective of this study is to introduce a generalized divergence measure integrated into the Technique for Order of Preference by Similarity to Ideal Solution (TOPSIS) approach. Given the inherent uncertainty and ambiguity in multi-criteria decision-making (MCDM) scenarios, the concept of the fuzzy α-cut is leveraged. This allows experts to establish a broader spectrum of rankings, accommodating fluctuations in their confidence levels. To produce consistent criteria weights with the existence of outliers, the fuzzy Method based on the Removal Effects of Criteria (MEREC) is employed. To showcase the viability and effectiveness of the proposed approach, a quantitative illustration is provided through a staff performance review. In this context, the findings are compared with other MCDM methodologies, considering correlation coefficients and CPU time. The results demonstrate that the proposed technique aligns with current distance measure approaches, with all correlation coefficient values exceeding 0.9. Notably, the proposed method also boasts the shortest CPU time when compared to alternative divergence measure methodologies. As a result, it becomes evident that the proposed technique yields more sensible and practical results compared to its counterparts in this category.
A Secure Authentication Protocol Supporting Efficient Handover for UAV
Authors: Wen Kang , Wang Shengbao , Wu Yixiao , Wang Jie , Han Lidong , Xie Qi
Published: February 2024

Unmanned Aerial Vehicles (UAVs) are increasingly pivotal in operations such as flood rescue, wildfire surveillance, and covert military endeavors, with their integration into the Internet of Things (IoT) networks broadening the scope of services they provide. Amidst this expansion, security concerns for UAVs have come to the forefront, particularly in open communication environments where they face authentication challenges and risks of sensitive data, including location information, being exposed to unauthorized parties. To address these issues, we propose a secure and lightweight authentication scheme that combines the use of anonymity mechanisms and Physical Unclonable Functions (PUFs). Specifically, we employ pseudo- and temporary identities to maintain the anonymity of UAVs, while also utilizing PUF technology to strengthen the security of Ground Station Servers (GSSs) against physical threats. Rigorous validation through ProVerif and the Random Oracle (ROR) Model indicates our scheme’s superior performance over existing protocols in terms of both efficiency and security.
Primary Liver Perivascular Epithelioid Cell Tumor (PEComa): Case Report and Literature Review
Authors: Kvietkauskas Mindaugas , Samuolyte Austeja , Rackauskas Rokas , Luksaite-Lukste Raminta , Karaliute Gintare , Maskoliunaite Vygante , Valkiuniene Ruta Barbora , Sokolovas Vitalijus , Strupas Kestutis
Published: February 2024

A primary liver perivascular epithelioid cell tumor (PEComa) is an extremely rare entity. In this article, we present a case report with a review of the literature on the patients diagnosed with primary liver PEComa and an elaboration of diagnostic and treatment modalities. A systematic literature search was conducted using the terms “perivascular epithelioid cell tumor”, “PEComa”, “liver”, and “hepatic”. All articles describing patients diagnosed with primary liver PEComa were included. We identified a total of 224 patients of primary liver PEComa from 75 articles and a case from the present study with a significant preponderance of females (ratio 4:1) and with a mean age of 45.3 ± 12.1 years. Most of the patients (114 out of 224, 50.9%) were asymptomatic. A total of 183 (81.3%) patients underwent surgical hepatic resection at the time of diagnosis, while 19 (8.4%) underwent surveillance. Recurrence and metastases were detected in seven (3.1%) and six (2.7%) patients, respectively. In conclusion, surgical resection remains the cornerstone of therapy; however, the presence of nonspecific imaging features makes it difficult to reach a definite diagnosis preoperatively. Therefore, a multidisciplinary approach should be the gold standard in selecting the treatment modality.
Unraveling Metabolic Changes following Stroke: Insights from a Urinary Metabolomics Analysis
Authors: Petersson Jamie N. , Bykowski Elani A. , Ekstrand Chelsea , Dukelow Sean P. , Ho Chester , Debert Chantel T. , Montina Tony , Metz Gerlinde A. S.
Published: February 2024

The neuropathological sequelae of stroke and subsequent recovery are incompletely understood. Here, we investigated the metabolic dynamics following stroke to advance the understanding of the pathophysiological mechanisms orchestrating stroke recovery. Using a nuclear magnetic resonance (NMR)-driven metabolomic profiling approach for urine samples obtained from a clinical group, the objective of this research was to (1) identify novel biomarkers indicative of severity and recovery following stroke, and (2) uncover the biochemical pathways underlying repair and functional recovery after stroke. Urine samples and clinical stroke assessments were collected during the acute (2–11 days) and chronic phases (6 months) of stroke. Using a 700 MHz 1H NMR spectrometer, metabolomic profiles were acquired followed by a combination of univariate and multivariate statistical analyses, along with biological pathway analysis and clinical correlations. The results revealed changes in phenylalanine, tyrosine, tryptophan, purine, and glycerophospholipid biosynthesis and metabolism during stroke recovery. Pseudouridine was associated with a change in post-stroke motor recovery. Thus, NMR-based metabolomics is able to provide novel insights into post-stroke cellular functions and establish a foundational framework for future investigations to develop targeted therapeutic interventions, advance stroke diagnosis and management, and enhance overall quality of life for individuals with stroke.
Functional Microorganisms Drive the Formation of Black-Odorous Waters
Authors: Wu Yuchen , Wang Wenjing , Liu Xiaozhu , Sheng Yanqing
Published: February 2024

Black-odorous waters are water bodies that are noticeably abnormal in color or emit unpleasant odors. River water pollution and ecological degradation have gradually emerged with urbanization and rapid economic development, and BOW has become frequent. The black-odorous evolution of urban water bodies is a serious environmental problem in many areas, posing a serious threat to both human health and the ecological environment. Functional microorganisms are closely related to the formation of black-odorous phenomena in water bodies, but the understanding of the mechanisms by which functional microorganisms influence the formation of BOW is very limited. In this study, water samples from the Guangdang River in Yantai, Shandong Province, China, were collected as the bacterial solution in the study, and how environmental factors and functional microorganisms affect the formation of black smelly water was investigated by artificially simulating black smelly water. The results indicated that different environmental factors have different effects on the formation of BOW. Anaerobic conditions accelerated the formation of BOW, and species diversity and species abundance were lowest under this condition. Hydraulic disturbance and nitrate effectively mitigated the BOW phenomenon, in which species diversity and species abundance were higher; controlling either of these variables was effective in mitigating the BOW phenomenon. Desulfobacterota played a key role in the formation of BOW, and reducing the proportion of Desulfobacterota in the microbial community could effectively improve the water quality. Possible directions of electron transfer in the process were hypothesized. This study contributes to identifying the biological driving factors for black-odorous evolution, presents insight for preventing BOW formation, and provides a scientific basis for subsequent BOW management.
New Age Constraints of the Bilong Co Oil Shale in the Qiangtang Basin, Northern Tibet: Evidence from In Situ U–Pb Dating and Palaeontology
Authors: Zhang Haowei , Wang Jian , Mansour Ahmed , Zhang Jianyong , Wei Hengye , Fu Xiugen , Shen Lijun , Xiong Shaoyun , Ahmed Mohamed S. , Gentzis Thomas
Published: February 2024

The Bilong Co oil shale is one of the most significant source rocks in the Mesozoic Qiangtang Basin (Northern Tibet); however, its absolute chronology remains controversial. In this study, in situ carbonate U–Pb isotope dating analysis was carried out for the first time. Detailed field geological investigations yielded some age-diagnostic ammonites, enabling a re-evaluation of the stratigraphic age of the Bilong Co oil shale. A total of 61 spots of U–Pb isotope dating from the middle part of the Bilong Co oil shale section suggests an average age of 181 ± 13 Ma. Elemental geochemistry and diagenetic analysis indicate that the proposed age represents the early deposition of the calcite, and the oil shale was deposited during the Early Jurassic time. This estimated age is further supported by the newly discovered ammonite assemblage of Hildoceratidae–Tiltoniceras sp. at the top part of the oil shale section, which confirms the deposition of the oil shale during the Toarcian age of the late Early Jurassic. Consequently, the Bilong Co oil shale can be assigned to the Quse Formation, which is attributed to the Lower Jurassic rather than the Middle Jurassic. The re-assessment of the stratigraphic age of the Bilong Co oil shale is of great significance for regional evaluation and exploration activities of hydrocarbon source rock layers in the Qiangtang Basin as well as for global stratigraphic correlation of the late Early Jurassic Toarcian oceanic anoxic event.
Thermochronology of the Laojunshan–Song Chai Granite Gneiss Massif (North Vietnam, South China)
Authors: Travin Alexey , Murzintsev Nikolai , Kruk Nikolai
Published: February 2024

A reconstruction of the tectonothermal evolution of the Laojunshan–Song Chai granite gneiss massif (North Vietnam, South China) was carried out, based on summaries of the latest isotopic and fission-track dating results. The recorded wide range (420–465 Ma) of the age of granite gneiss rocks testifies to the long-term existence of a partially molten layer at a depth of 20–30 km for several tens of Ma. By the Devonian–early Carboniferous, a section of the excessively thickened crust was denudated, the massif was exhumated to the level of the upper crust, and isotope systems were “frozen”. The rate of uplift of the rocks of the massif is estimated to be about 0.2–0.5 mm/year. In the further history of the granite gneiss massif, episodes of repeated burial to a depth of about 13 km are recorded, associated with the Indosinian collision. The rocks have experienced metamorphism of the amphibolite-green schist facies, accompanied by tectonic transport in the form of a thrust sheet. Over the next 200 Ma, the uplift of the massif and the erosion of the overlying strata occurred in discrete pulses, during a sequence of active tectonic events. Thus, the thermochronological and P-T history of the Laojunshan–Song Chai massif is a kind of chronicle of regional tectonic–thermal events. In the history of the massif, traces of two orogenic cycles associated with the collision of the Cathaysia and Yangtze blocks in the Lower Paleozoic and the Indosinian collision in the Triassic are recorded.
Insight into the Storage Mechanism of Sandwich-Like Molybdenum Disulphide/Carbon Nanofibers Composite in Aluminum-Ion Batteries
Authors: Wang Xiaobing , Zhuang Ruiyuan , Liu Xinyi , Hu Mingxuan , Shen Panfeng , Luo Jintao , Yang Jianhong , Wu Jianchun
Published: February 2024

Aluminum-ion batteries (AIBs) have become a research hotspot in the field of energy storage due to their high energy density, safety, environmental friendliness, and low cost. However, the actual capacity of AIBs is much lower than the theoretical specific capacity, and their cycling stability is poor. The exploration of energy storage mechanisms may help in the design of stable electrode materials, thereby contributing to improving performance. In this work, molybdenum disulfide (MoS2) was selected as the host material for AIBs, and carbon nanofibers (CNFs) were used as the substrate to prepare a molybdenum disulfide/carbon nanofibers (MoS2/CNFs) electrode, exhibiting a residual reversible capacity of 53 mAh g−1 at 100 mA g−1 after 260 cycles. The energy storage mechanism was understood through a combination of electrochemical characterization and first-principles calculations. The purpose of this study is to investigate the diffusion behavior of ions in different channels in the host material and its potential energy storage mechanism. The computational analysis and experimental results indicate that the electrochemical behavior of the battery is determined by the ion transport mechanism between MoS2 layers. The insertion of ions leads to lattice distortion in the host material, significantly impacting its initial stability. CNFs, serving as a support material, not only reduce the agglomeration of MoS2 grown on its surface, but also effectively alleviate the volume expansion caused by the host material during charging and discharging cycles.
Longitudinal Relationship between the Percentage of Energy Intake from Macronutrients and Overweight/Obesity among Chinese Adults from 1991 to 2018
Authors: Yuan Xiaorong , Wei Yanli , Jiang Hongru , Wang Huijun , Wang Zhihong , Dong Mengru , Dong Xiaohui , Zhang Jiguo
Published: February 2024

To investigate the prospective relationship between macronutrient intake and overweight/obesity, data were collected in the China Health and Nutrition Survey (CHNS) from 1991 to 2018. Adults who participated in at least two waves of the survey and were not obese at baseline were selected as the study subjects. A total of 14,531 subjects were finally included with complete data. Overweight/obesity was defined as a body mass index (BMI) ≥ 24.0 kg/m2. The generalized estimating equation (GEE) was used to analyze the relationship between the percentage of energy intake from macronutrients and BMI and overweight/obesity. The percentages of energy intake from protein and fat showed an increasing trend (p < 0.01), and the percentage of energy intake from carbohydrate showed a decreasing trend (p < 0.01) among Chinese adults between 1991 and 2018. Adjusting for covariates, the energy intake from fat was positively correlated with BMI, while the energy intake from carbohydrates was negatively correlated with BMI. The percentage of energy intake from non-high-quality protein and polyunsaturated fatty acids (PUFA) were positively correlated with overweight/obesity. In contrast, monounsaturated fatty acids (MUFA) and high-quality carbohydrates were negatively correlated with overweight/obesity. In short, fat, non-high-quality protein, saturated fatty acids (SFA), and PUFA were positively correlated with the risk of obesity, whereas higher carbohydrate, MUFA, and high-quality carbohydrate intake were associated with a lower risk of obesity. Obesity can be effectively prevented by appropriately adjusting the proportion of intake from the three major macronutrients.
The Role of the Dietitian within Family Therapy for Anorexia Nervosa (FT-AN): A Reflexive Thematic Analysis of Child and Adolescent Eating Disorder Clinician Perspectives
Authors: Brennan Cliona , Baudinet Julian , Simic Mima , Eisler Ivan
Published: February 2024

Background: Despite dietitians being important members of the multidisciplinary team delivering family therapy for anorexia nervosa (FT-AN), their specific responsibilities and roles are unclear and their involvement in the treatment can be a contentious issue. Methodology: Clinicians (n = 20) experienced in the delivery of FT-AN who were working at a specialist child and adolescent eating disorder service responded to an online survey about their experience of including a dietitian in FT-AN and how they understand the role. Both categorical and open-ended questions were used. Reflexive thematic analysis was used to analyse the qualitative free-text responses of clinician perspectives on the role of the dietitian in FT-AN. Results: All clinicians agreed that dietetics had a role within FT-AN and most frequently sought dietetic involvement in the early phases of FT-AN. Reflexive thematic analysis of responses identified three main themes. These were (1) collaboration is key, (2) confidence as a core consideration and (3) case-by-case approach. These themes evidenced the role of the dietitian within FT-AN and highlighted both the benefits and concerns of this involvement. Conclusions: This study demonstrated that dietitians can take a core role as collaborators within therapy-led teams that facilitate joint working and sharing of expertise. However, dietetic input should be considered on a case-by-case basis, given its potential for creating an over-focus on nutrition and potentially diminishing parental confidence in feeding. When indicated for selected cases, nutritional counselling should be offered in joint sessions with the therapist rather than separately. The findings of the study were limited by the small sample size of participants recruited from a single centre and heterogeneity in the professional background of respondents. Although the integration of dietetics within the multidisciplinary team and the ability of dietitians to individualise patient care can enhance FT-AN treatment, potential benefits and disbenefits should be considered for each case.
Associations of Triglycerides and Atherogenic Index of Plasma with Brain Structure in the Middle-Aged and Elderly Adults
Authors: Chen Xixi , Bao Yujia , Zhao Jiahao , Wang Ziyue , Gao Qijing , Ma Mingyang , Xie Ziwen , He Mu , Deng Xiaobei , Ran Jinjun
Published: February 2024

Triglyceride (TG) and atherogenic index of plasma (AIP) have been acknowledged to be risk factors for vascular insults, but their impacts on the brain system remain elusive. To fill in some gaps, we investigated associations of TG and AIP with brain structure, leveraging the UK Biobank database. TG and high-density lipoprotein cholesterol (HDL-C) were examined at baseline and AIP was calculated as log (TG/HDL-C). We build several linear regression models to estimate associations of TG and AIP with volumes of brain grey matter phenotypes. Significant inverse associations of TG and AIP with volumes of specific subcortical traits were observed, among which TG and AIP were most significantly associated with caudate nucleus (TG: β [95% confidence interval CI] = −0.036 [−0.051, −0.022], AIP: −0.038 [−0.053, −0.023]), thalamus (−0.029 [−0.042, −0.017], −0.032 [−0.045, −0.019]). Higher TG and AIP were also considerably related with reduced cortical structure volumes, where two most significant associations of TG and AIP were with insula (TG: −0.035 [−0.048, −0.022], AIP: −0.038 [−0.052, −0.025]), superior temporal gyrus (−0.030 [−0.043, −0.017], −0.033 [−0.047, −0.020]). Modification effects of sex and regular physical activity on the associations were discovered as well. Our findings show adverse associations of TG and AIP with grey matter volumes, which has essential public health implications for early prevention in neurodegenerative diseases.
Polyphenols Influence the Development of Endometrial Cancer by Modulating the Gut Microbiota
Authors: Baranowska-Wójcik Ewa , Winiarska-Mieczan Anna , Olcha Piotr , Kwiecień Małgorzata , Jachimowicz-Rogowska Karolina , Nowakowski Łukasz , Miturski Andrzej , Gałczyński Krzysztof
Published: February 2024

Dysbiosis of the microbiota in the gastrointestinal tract can induce the development of gynaecological tumours, particularly in postmenopausal women, by causing DNA damage and alterations in metabolite metabolism. Dysbiosis also complicates cancer treatment by influencing the body’s immune response and disrupting the sensitivity to chemotherapy drugs. Therefore, it is crucial to maintain homeostasis in the gut microbiota through the effective use of food components that affect its structure. Recent studies have shown that polyphenols, which are likely to be the most important secondary metabolites produced by plants, exhibit prebiotic properties. They affect the structure of the gut microbiota and the synthesis of metabolites. In this review, we summarise the current state of knowledge, focusing on the impact of polyphenols on the development of gynaecological tumours, particularly endometrial cancer, and emphasising that polyphenol consumption leads to beneficial modifications in the structure of the gut microbiota.
Trends in Faecal Zonulin Concentrations in Paediatric Patients with Celiac Disease at Baseline and on a Gluten-Free Diet: Exploring Correlations with Other Faecal Biomarkers
Authors: Martínez Gallego Miguel Ángel , Crespo Sánchez María Gema , Serrano Olmedo María Gemma , Buño Soto Antonio , Álvarez Casasempere Sonia , Nozal Pilar , Martínez-Ojinaga Eva , Molina Arias Manuel , Losantos-García Itsaso , Molero-Luis Marta
Published: February 2024

Celiac disease (CeD) is an autoimmune condition triggered by gluten in genetically predisposed individuals, affecting all ages. Intestinal permeability (IP) is crucial in the pathogenesis of CeD and it is primarily governed by tight junctions (TJs) that uphold the intestinal barrier’s integrity. The protein zonulin plays a critical role in modulating the permeability of TJs having emerged as a potential non-invasive biomarker to study IP. The importance of this study lies in providing evidence for the usefulness of a non-invasive tool in the study of IP both at baseline and in the follow-up of paediatric patients with CeD. In this single-centre prospective observational study, we explored the correlation between faecal zonulin levels and others faecal and serum biomarkers for monitoring IP in CeD within the paediatric population. We also aimed to establish reference values for faecal zonulin in the paediatric population. We found that faecal zonulin and calprotectin values are higher at the onset of CeD compared with the control population. Specifically, the zonulin levels were 347.5 ng/mL as opposed to 177.7 ng/mL in the control population (p = 0.001), while calprotectin levels were 29.8 μg/g stool compared to 13.9 μg/g stool (p = 0.029). As the duration without gluten consumption increased, a significant reduction in faecal zonulin levels was observed in patients with CeD (348.5 ng/mL vs. 157.1 ng/mL; p = 0.002), along with a decrease in the prevalence of patients with vitamin D insufficiency (88.9% vs. 77.8%). We conclude that faecal zonulin concentrations were higher in the patients with active CeD compared with healthy individuals or those following a gluten-free diet (GFD). The significant decrease in their values over the duration of the GFD suggests the potential use of zonulin as an additional tool in monitoring adherence to a GFD.
The Impact of Asymmetric Dark Matter on the Thermal Evolution of Nucleonic and Hyperonic Compact Stars
Authors: Giangrandi Edoardo , Ávila Afonso , Sagun Violetta , Ivanytskyi Oleksii , Providência Constança
Published: February 2024

We investigate the impact of asymmetric fermionic dark matter (DM) on the thermal evolution of neutron stars (NSs), considering a scenario where DM interacts with baryonic matter (BM) through gravity. Employing the two-fluid formalism, our analysis reveals that DM accrued within the NS core exerts an inward gravitational pull on the outer layers composed of BM. This gravitational interaction results in a noticeable increase in baryonic density within the core of the NS. Consequently, it strongly affects the star’s thermal evolution by triggering the early onsets of the direct Urca (DU) processes, causing enhanced neutrino emission and rapid star cooling. Moreover, the photon emission from the star’s surface is modified due to a reduction in radius. We demonstrate the effect of DM gravitational pull on nucleonic and hyperonic DU processes that become kinematically allowed even for NSs of low mass. We then discuss the significance of observing NSs at various distances from the Galactic center. Given that the DM distribution peaks toward the Galactic center, NSs within this central region are expected to harbor higher fractions of DM, potentially leading to distinct cooling behaviors.
Pathogenicity Analyses of Rice Blast Fungus (Pyricularia oryzae) from Japonica Rice Area of Northeast China
Authors: Wang Dongyuan , Zhu Feng , Wang Jichun , Ju Hongguang , Yan Yongfeng , Qi Shanyan , Ou Yuping , Tian Chengli
Published: February 2024

In order to understand the pathogenicity differentiation of rice blast fungus (Pyricularia oryzae Cavara), a total of 206 isolates of P. oryzae were collected from three Japonica rice regions in Jilin Province, northeast China. Pathogenicity test showed that the reaction pattern of 25 monogenic differential varieties (MDVs) of rice (Oryza sativa L.) demonstrated a wide pathogenic diversity among the isolates. Those MDVs harbor 23 resistance (R) genes with the susceptible variety Lijiangxintuanheigu (LTH) as control. Virulent isolates of MDVs harboring R genes Pish, Pit, Pia, Pii, Pik-s, Pik, Pita (two lines), and Pita-2 (two lines) had high frequencies ranging from 80 to 100%, to MDVs harboring R genes Pib, Pi5(t), Pik-m, Pi1, Pik-h, Pik-p, Pi7(t), Piz, Piz-5, and Piz-t showed intermediate frequencies ranging from 40 to 80%, and to MDVs with R genes Pi3, Pi9(t), Pi12(t), Pi19(t) and Pi20(t) presented low frequencies ranging only from 0 to 40%. The U-i-k-z-ta pattern of race-named criteria categorized the 206 isolates into 175 races. Sub-unit U73 for Pib, i7 for Pi3 and Pi5(t), k177 for Pik-m/Pik-h/Pik-p, z17 for Pi9(t), and ta332 for Pi20(t) were crucial on pathogenic differences in regions. Twenty-seven standard differential blast isolates (SDBIs) were selected to characterize resistance in rice accessions. This study could help to build a durable identification system against blast in the Japonica rice area of northeast China and enhance our understanding of the differentiation and diversity of blast races in the world.
Hepatitis C Epidemiology: Insights from a Comprehensive Cohort Study in ASST Melegnano and Martesana, Lombardia Region, Northern Italy
Authors: Nardone Michele , Di Stasio Dario , Lucchese Alberta , Gentili Daniele , Cattabianchi Giulia , Signorelli Carlo , Sarchi Pierangelo , Pulcrano Giovanna , Lembo Valentino , Pirola Paola Maria , Lauritano Dorina , Carinci Francesco
Published: February 2024

Hepatitis C virus (HCV) infection is a significant public health problem affecting 58 million people worldwide, including 3.9 million in Europe. Many of these infections go undiagnosed because chronic infection is often asymptomatic. This observational cohort study presents a detailed examination of hepatitis C virus epidemiology in Lombardia (Italy) and was conducted within the ASST “Melegnano e della Martesana”. The study involved comprehensive HCV screening of 3290 patients accessing the collection points and/or hospitalized in the facilities of the ASST from 20 May 2022 to 13 April 2023. Screening was conducted using serological assays. The prevalence of anti-HCV-positive patients (HCV-Ab) and then HCV-positive patients (RNA) was calculated. Chi-square tests examined the associations between continuous and categorical variables. Logistic regression was used to evaluate the influence of demographic and geographic variables as predictors of HCV positivity. The study revealed an overall HCV-Ab prevalence of 0.912% (CI (0.59–1.24%)) in the examined cohort, of whom 15.15% (two females and three males) were positive for HCV RNA. The prevalence of HCV RNA positivity was 0.152% (CI (0.05–0.35%)). Sex disparity was evident, with male patients exhibiting a higher prevalence compared to females, confirmed by logistic regression (0.0147 vs. 0.0061–OR = 2.44; CI (0.0059–0.0124)). Age stratification indicated an ascending trend in prevalence with age, peaking at 1.35% in individuals aged over 50. These findings underscore the critical need for targeted HCV screening, contributing valuable insights to the global epidemiology of HCV in the era of DAAs.
Inhibition of miR-33a-5p in Macrophage-like Cells In Vitro Promotes apoAI-Mediated Cholesterol Efflux
Authors: Oladosu Olanrewaju , Chin Emma , Barksdale Christian , Powell Rhonda R. , Bruce Terri , Stamatikos Alexis
Published: February 2024

Atherosclerosis is caused by cholesterol accumulation within arteries. The intima is where atherosclerotic plaque accumulates and where lipid-laden foam cells reside. Intimal foam cells comprise of both monocyte-derived macrophages and macrophage-like cells (MLC) of vascular smooth muscle cell (VSMC) origin. Foam cells can remove cholesterol via apoAI-mediated cholesterol efflux and this process is regulated by the transporter ABCA1. The microRNA miR-33a-5p is thought to be atherogenic via silencing ABCA1 which promotes cholesterol retention and data has shown inhibiting miR-33a-5p in macrophages may be atheroprotective via enhancing apoAI-mediated cholesterol efflux. However, it is not entirely elucidated whether precisely inhibiting miR-33a-5p in MLC also increases ABCA1-dependent cholesterol efflux. Therefore, the purpose of this work is to test the hypothesis that inhibition of miR-33a-5p in cultured MLC enhances apoAI-mediated cholesterol efflux. In our study, we utilized the VSMC line MOVAS cells in our experiments, and cholesterol-loaded MOVAS cells to convert this cell line into MLC. Inhibition of miR-33a-5p was accomplished by transducing cells with a lentivirus that expresses an antagomiR directed at miR-33a-5p. Expression of miR-33a-5p was analyzed by qRT-PCR, ABCA1 protein expression was assessed via immunoblotting, and apoAI-mediated cholesterol efflux was measured using cholesterol efflux assays. In our results, we demonstrated that lentiviral vector-mediated knockdown of miR-33a-5p resulted in decreasing expression of this microRNA in cultured MLC. Moreover, reduction of miR-33a-5p in cultured MLC resulted in de-repression of ABCA1 expression, which caused ABCA1 protein upregulation in cultured MLC. Additionally, this increase in ABCA1 protein expression resulted in enhancing ABCA1-dependent cholesterol efflux through increasing apoAI-mediated cholesterol efflux in cultured MLC. From these findings, we conclude that inhibiting miR-33a-5p in MLC may protect against atherosclerosis by promoting ABCA1-dependent cholesterol efflux.
Shared Pathogenic and Therapeutic Characteristics of Endometriosis, Adenomyosis, and Endometrial Cancer: A Comprehensive Literature Review
Authors: Mitranovici Melinda-Ildiko , Chiorean Diana Maria , Moraru Liviu , Moraru Raluca , Caravia Laura , Tiron Andreea Taisia , Cotoi Titiana Cornelia , Toru Havva Serap , Cotoi Ovidiu Simion
Published: February 2024

Endometriosis and adenomyosis behave similarly to cancer. No current treatments represent a cure, even if there are several options, including hormonal and surgical therapy. In advanced or recurrent pathologies, however, personalized treatment is necessary. We have found that due to the multiple common features, various therapeutic options have been used or studied for all three pathologies, with varying results. The objective of this review is to extract from the relevant literature the compounds that are used for endometriosis and adenomyosis characterized by malignant behavior, with some of these drugs being studied first in the treatment of endometrial cancer. Special attention is needed in the pathogenesis of these pathologies. Despite the multiple drugs that have been tested, only a few of them have been introduced into clinical practice. An unmet need is the cure of these diseases. Long-time treatment is necessary because symptoms persist, and surgery is often followed by postoperative recurrence. We emphasize the need for new, effective, long-term treatments based on pathogeny while considering their adverse effects.
Senescence and SASP Are Potential Therapeutic Targets for Ischemic Stroke
Authors: Ouvrier Blake , Ismael Saifudeen , Bix Gregory Jaye
Published: February 2024

Aging is a known co-morbidity of ischemic stroke with its risk and severity increasing every year past 55+. While many of the current stroke therapies have shown success in reducing mortality, post-stroke morbidity has not seen the same substantial reduction. Recently, the involvement of cellular senescence and SASP in brain injury and neurological degeneration has been recognized. Ischemic injury causes oxidative stress and mitochondrial damage that induces senescence through the activation of p21 and p16 pathways, ultimately leading to synthesis and release of senescence-associated secretory phenotype (SASP). This ischemic event causes stress-induced premature senescence (SIPS), aging the brain decades beyond the standard biological age due to an increase in senescent cells in the ischemic core and ipsilateral hemisphere. Therefore, therapies that target the senescent cells and SASP, including senolytics, senomorphic drugs, stem cell therapies, and other cell-specific interventions, may be a new path for stroke treatment.
Transpiration Dynamics of Esparto Grass (Macrochloa tenacissima (L.) Kunth) in a Semi-Arid Mediterranean Climate: Unraveling the Impacts of Pine Competition
Authors: Pérez-Anta Iván , Rubio Eva , López-Serrano Francisco Ramón , Garcés Diego , Andrés-Abellán Manuela , Picazo Marta , Chebbi Wafa , Arquero Rocío , García-Morote Francisco Antonio
Published: February 2024

Macrochloa tenacissima (M. tenacissima), or esparto, is a perennial tussock grass that coexists with Pinus halepensis (P. halepensis) in semi-arid Mediterranean woodlands. This research was carried out to explore diurnal transpiration at leaf level in esparto grass under different levels of pine–esparto competition and in contrasting environmental soil water conditions. The measurement period spanned from the summer of 2020 to the spring of 2021. The relationship between transpiration and competition was conducted in open and closed P. halepensis stands, and the type of leaf (green, senescent) and the maturity of the esparto grass were taken into account. We observed a higher control of transpiration in green leaves, and the correlations between the transpiration and pine competition were noted exclusively in this type of leaf. Our results demonstrated a significant impact of pine competitors (closed stands) on the transpiration of esparto grass, particularly during seasons characterized by scenarios of high water demand: the summer drought period and the commencement of the growing and flowering period (spring). Furthermore, our findings revealed a greater response to transpiration in mature bushes compared to young ones under severe water stress, indicating a higher adaptation to drought by esparto as it ages. Although our results confirmed that PAR increased transpiration in all seasons and in both stands, which is attributable to the heliophilia of esparto grass, the site effects on transpiration could also be attributable to competition for water, especially during periods of drought. These results may have important implications for the dynamics and management of these semi-arid mixed woodlands, as well as the planning of reforestation programs aimed at restoring esparto grass formations.
Development of an NLR-ID Toolkit and Identification of Novel Disease-Resistance Genes in Soybean
Authors: Shao Wei , Shi Gongfu , Chu Han , Du Wenjia , Zhou Zikai , Wuriyanghan Hada
Published: February 2024

The recognition of pathogen effectors through the nucleotide-binding leucine-rich repeat receptor (NLR) family is an important component of plant immunity. In addition to typical domains such as TIR, CC, NBS, and LRR, NLR proteins also contain some atypical integrated domains (IDs), the roles of which are rarely investigated. Here, we carefully screened the soybean (Glycine max) genome and identified the IDs that appeared in the soybean TNL-like proteins. Our results show that multiple IDs (36) are widely present in soybean TNL-like proteins. A total of 27 Gm-TNL-ID genes (soybean TNL-like gene encoding ID) were cloned and their antiviral activity towards the soybean mosaic virus (SMV)/tobacco mosaic virus (TMV) was verified. Two resistance (R) genes, SRA2 (SMV resistance gene contains AAA_22 domain) and SRZ4 (SMV resistance gene contains zf-RVT domain), were identified to possess broad-spectrum resistance characteristics towards six viruses including SMV, TMV, plum pox virus (PPV), cabbage leaf curl virus (CaLCuV), barley stripe mosaic virus (BSMV), and tobacco rattle virus (TRV). The effects of Gm-TNL-IDX (the domain of the Gm-TNL-ID gene after the TN domain) on the antiviral activity of a R protein SRC7TN (we previously reported the TN domain of the soybean broad-spectrum resistance gene SRC7) were validated, and most of Gm-TNL-IDX inhibits antiviral activity mediated by SRC7TN, possibly through intramolecular interactions. Yeast-two-hybrid (Y2H) and bimolecular fluorescence complementation (BiFC) assays showed that seven Gm-TNL-IDX interacted with SMV-component proteins. Truncation analysis on a broad-spectrum antiviral protein SRZ4 indicated that SRZ4TIR is sufficient to mediate antiviral activity against SMV. Soybean cDNA library screening on SRZ4 identified 48 interacting proteins. In summary, our results indicate that the integration of IDs in soybean is widespread and frequent. The NLR-ID toolkit we provide is expected to be valuable for elucidating the functions of atypical NLR proteins in the plant immune system and lay the foundation for the development of engineering NLR for plant-disease control in the future.
Modulating Thermal Conductivity and Flame Retardancy of Polyolefin Composites via Distributed Structures of Magnesium Hydroxide and Hexagonal Boron Nitride
Authors: Wang Qi , Pan Lin , Bo Ruitian , Wang Yunfei , Han Zhidong
Published: February 2024

Thermally conductive and flame-retardant polyolefin composites are facing great challenges in meeting the increasing demands for fire safety and thermal management. Aiming at simultaneously enhancing thermal conductivity and flame retardancy, hexagonal boron nitride (hBN) and magnesium hydroxide (MH) were adopted in ethylene–vinyl acetate copolymer/polyolefin elastomer (EVA/POE) blends to design composites with selective filler distributions and co-continuous networks via different processing schemes. The thermal conductivity and flame retardancy show strong dependence on the distributed structure of hBN and MH. The composites with hBN-rich centers and MH-rich edges in the filled POE phase show a thermal conductivity of 0.70 W/(m·K) and an LOI of 27.7%, which are very close to the thermal conductivity of EVA/POE/hBN and the LOI of EVA/POE/MH at the same total filler content. The composites with MH-rich centers and hBN-rich edges show pHRR, THR and TSP values of 169 kW/m2, 49.8 MJ/m2 and 1.8 m2, which are decreased by 40%, 33% and 62% in comparison with EVA/POE/MH, respectively. Modulating the filler structure distribution provides a strategy to co-enhance thermal conductivity and flame retardancy.
Evaluating the Incorporation of Myrtus communis L. Leaves Infusion in Alginate-Based Films and Spheres to Enhance the Oxidative Stability of Oil-in-Water Emulsions
Authors: El Hammadi Nisserine , Almajano María Pilar , Pastor Maria Vicenta , Codina-Torrella Idoia
Published: February 2024

Myrtus communis L. is a species of the Myrtaceae family that is found in the Mediterranean region, and it is traditionally recognized for its importance and different uses. The objective of this study was to determine the effect of M. communis L. leaf extract (MCLE), which was incorporated directly into alginate spheres and films, on preserving oil-in-water emulsions from oxidation. For this purpose, the solvent extraction (with ethanol at 40, 60, and 80%) of the antioxidant compounds was optimized (total phenolic compounds (TPCs) and total flavonoid content (TFC)) along with the scavenging activity. The best condition for the extraction corresponded with 60% ethanol (MCLE60), with a TPC of ~66.06 g GAE/L and a TFC of ~18.91 g QE/L, which was selected for use in the following assays. MCLE60 showed a considerable radical scavenging activity (24.85 mmol TE/L in FRAP, 28.75 mmol TE/L in DPPH, 30.61 mmol TE/L in ABTS, and 14.94 mmol TE/L in ORAC), which was probably due to its content in the phenolic compounds arbutin (122.08 mg/L), epicatechin (73.89 mg/L), sinapic acid (51.85 mg/L), and gallic acid (36.72 mg/L). The oil-in-water emulsions with the MCLE60 spheres showed the best oxidative stability (TBARS ~2.64 mg MDA/kg of emulsion, PV ~35.7 meq hydroperoxides/kg of emulsion) in comparison to the control. The film was also able to protect the emulsion from oxidation for more than a week at 30 °C (TBARS ~1.9 mg MDA/kg of emulsion). The alginate films with MCLE60 presented an important release of phenolic compounds in water and acetic food simulants, while in both ethanol simulants, the release of TPC remained more stable over time. Thus, this study highlights the potential uses of MCLE as a natural ingredient for emulsion oxidative preservation and the production of alginate delivery systems (spheres and films).
Additive and Lithographic Manufacturing of Biomedical Scaffold Structures Using a Versatile Thiol-Ene Photocurable Resin
Authors: Kainz Michael , Perak Stjepan , Stubauer Gerald , Kopp Sonja , Kauscheder Sebastian , Hemetzberger Julia , Martínez Cendrero Adrián , Díaz Lantada Andrés , Tupe Disha , Major Zoltan , Hanetseder Dominik , Hruschka Veronika , Wolbank Susanne , Marolt Presen Darja , Mühlberger Michael , Guillén Elena
Published: February 2024

Additive and lithographic manufacturing technologies using photopolymerisation provide a powerful tool for fabricating multiscale structures, which is especially interesting for biomimetic scaffolds and biointerfaces. However, most resins are tailored to one particular fabrication technology, showing drawbacks for versatile use. Hence, we used a resin based on thiol-ene chemistry, leveraging its numerous advantages such as low oxygen inhibition, minimal shrinkage and high monomer conversion. The resin is tailored to applications in additive and lithographic technologies for future biofabrication where fast curing kinetics in the presence of oxygen are required, namely 3D inkjet printing, digital light processing and nanoimprint lithography. These technologies enable us to fabricate scaffolds over a span of six orders of magnitude with a maximum of 10 mm and a minimum of 150 nm in height, including bioinspired porous structures with controlled architecture, hole-patterned plates and micro/submicro patterned surfaces. Such versatile properties, combined with noncytotoxicity, degradability and the commercial availability of all the components render the resin as a prototyping material for tissue engineers.