bluuebunny/crossref_metadata_2025 huggingface dataset:
Drowning in data? - publication and rescue archaeology in the 1990s
Authors: Thomas Roger
Published: January 2016

In a characteristically stimulating recent article in ANTIQUITY, Barry Cunliffe has touched on many of the most important issues concerning the publication of ‘rescue’ excavations in Britain in the 1990s (Cunliffe 1990). The purpose of the present article is to follow up some the points which Cunliffe has raised.

Publication, and the dissemination of information, is the lifeblood of any academic discipline, and questions of what is published (and of what is read!), where, how and by whom are of central importance for archaeology. Over the past two decades in Britain, and particularly in England where the volume of work has been greatest, there has been a recurrent concern with the problem of how to publish the results of ‘rescue’ archaeology. Rescue excavations can generate very large quantities of data, collected for reasons which are often largely beyond archaeological control, and the problems (both intellectual and practical) of publishing this material are considerable. In Britain the issues have been the subject of expert examination on two occasions since 1970 -the Frere (1975) and Cunliffe (1983) reports - and now in the 1990s the topic is firmly on the archaeological agenda again. This paper is intended as a contribution to the continuing debate.
Hunter-gatherer complexity on the west coast of North America
Authors: Maschner Hergert D. G. , Fagan Brian M.
Published: January 2016

The west coast of North America encompasses some of the richest and most diverse maritime environments on earth. Even in their presentday impoverished state, they support major commercial fisheries, large whale migrations and dense sea mammal populations. From the earliest days of European exploration, visitors such as the redoubtable Captain James Cook commented on the rich culture of Pacific coast peoples (Beaglehole 1967). ‘Their life may be said to comprise a constant meal,’ remarked Spanish friar Pedro Fages of the Chumash peoples of the Santa Barbara Channel in southern California. At European contact, between the 16th and 18th centuries AD, the shores of the Bering Strait, the Pacific Northwest and parts of the California coast supported elaborate, sophisticated and sedentary huntergatherer peoples. These decimated and muchchanged societies still enjoyed elaborate ceremonials and intricate social relations as late as the late 19th and early 20th centuries, when pioneer anthropologists such as Franz Boas and John Harrington worked among them. From these researches have come classic stereotypes of west coast peoples as ‘complex huntergatherer societies’, some of which were organized in powerful chiefdoms. Peoples like the Tlingit, the Kwakiutl and the Chumash have become the epitome of complex huntergatherers in many archaeologists’ eyes.
Physical anthropological evidence for the evolution of social complexity in coastal Southern California
Authors: Lambert Patricia M. , Walker Phillip L.
Published: January 2016

In this paper we use osteological data to evaluate theories about the rise of chiefdoms in southern California. To do this, we examine skeletal evidence for changes in diet, disease and violence in Santa Barbara Channel area populations. These collections date from before and after the development of large, sedentary coastal villages and a political system that facilitated inter-village economic interaction. Our data show that the health consequences of the development of these chiefdoms are comparable to those seen with the development of complex agricultural societies. They also provide insights into the causes of social complexity in non-agricultural societies.
The emergence of cultural complexity on the west coast of North America
Authors: Fagan Brian M. , Maschner Herbert D. G.
Published: January 2016

It has been apparent for some time that the overbearingly rich ethnographic record from the west coast has had an almost numbing effect on thinking about later prehistory from Alaska to the Mexican border. ‘The tyranny of the ethnographic record’ has been a war-cry in archaeology for some time, so there is nothing new in this observation. ‘I hear very little about olden times’, said Franz Boas of the Kwakiutl as long ago as the 1880s, but the archaeologists that followed in his footsteps seem to have forgotten his remark. There has been a tendency to think of the ethnographic record of the 17th-19th centuries AD as a true record of the state of native American society along the west coast before European contact. As Maschner and Ames point out in their papers, nothing could be further from the truth. Ann Ramenovsky (1984), among others, has pointed out that indigenous populations were decimated by smallpox and other infectious diseases very soon after contact, and sometimes even before actual physical meetings with the newcomers. Some of the elaborate cultures observed by Boas and others were, in themselves, responses to changing conditions. The elaboration of the Northwest potlatch is, of course, a well known example of this phenomenon. Here, Ames and Maschner point out that populations may already have been in decline prior to Columbus.
Excavations at Matupi cave
Authors: Noten Francis Van
Published: January 2016

The Matupi cave is one of the 40 caves within the Mount Hoyo limestone massif, a touristic site in Ituri, Zaïre. The author recognized its archaeological possibilities in February 1973 during a prospection tour in the northern part of the country. This expedition had been organized by the Institut des Musées nationaux du ZaYre in collaboration with the Musée royal de l'Afrique centrale at Tervuren, Belgium. Upon the visit to the cave a I sq m trial trench was excavated. More extended excavations (10 sq m) took place during January-March 1974. The excavations were carried out in spits of 5 cm on a I m grid system. Matupi is a large cave with an ideal living room at its entrance (c. 7 m high, c. 8 m deep and c. 5 m wide).
The Roman site of Stonea, Cambridgeshire
Authors: Potter T. W. , Jackson R. P. J.
Published: January 2016

The East Anglian Fenland comprises some 1,300 sq. miles (205 ha) of flat and monotonous terrain which is today cultivated on an intensive scale. Beneath this rich farmland lies a complex sequence of post-glacial deposits, reflecting periods of marine and fresh-water flooding, interspersed with phases of relative dryness (Godwin, 1978). During the Iron Age, the sea covered the northern Fenland with a thick blanket of silt, accounting for the dearth of finds of this time; but by c. AD 50 the sea was receding and the land had begun to dry out. Settlement soon followed. It is likely that a fort of the post-Boudiccan period was briefly in garrison at Grandford, north-west of March, but the principal remains are those of salt-making sites and of small farms. During the reign of Hadrian, however, both the scale and the nature of settlement changed dramatically. AS the authoritative survey of the region makes clear (Phillips, 1970), the 120s and 130s witnessed a sharp increase in the number of sites, including the foundation of many villages. At the same time a network of canals, designed to disperse water at times of flood, was constructed (Simmons, 1979), as well as a series of straight-line roads and drove-ways. Although there was no formal division of the land by centuriation, as had been customary in areas reclaimed earlier for the settlement of veteran—the Po Valley and Lake Velinus near Rieti provide obvious Italian examples—the hand of government is nevertheless explicit in the size of the undertaking. While we lack direct evidence, there can be little real doubt that the Fenland was taken over as Imperial land and placed in the charge of a procurator (Salway, 1970).
RESPONSE OF WHEAT (TRITICUM AESTIVUM) TO NITROGEN FERTILIZATION UNDER VARYING TILLAGE AND CROP ESTABLISHMENT PRACTICES IN GREENGRAM (VIGNA RADIATA)–WHEAT CROPPING SYSTEM
Authors: SHARMA A. R. , BEHERA U. K.
Published: January 2016

Wheat grown under zero tillage (ZT) and raised-bed following a legume crop may have a variable N requirement compared with conventionally-tilled flat-sown crop. A field experiment was conducted for two years during 2005–07 to study the effect of varying tillage (conventional and zero), crop establishment (flat and raised-bed sowing) and N fertilization (0–160 kg N ha−1) on wheat grown after greengram at New Delhi, India. Rainy-season greengram performed equally well under flat and raised-bed planting, but the seed yield was 25.9% lower under ZT than conventional tillage (CT) conditions. Wheat following greengram during winter season also showed better growth and yield under CT than ZT, as well as under flat sowing than furrow-irrigated raised-bed (FIRB) system. The grain yield was highest under CT-flat, and decreased by 5.4–9.4% under FIRB system. Nitrogen fertilization up to 120 kg N ha−1under CT and 160 kg N ha−1under ZT increased grain yield, and the optimum doses were worked out to be 147.1 and 154.2 kg ha−1, respectively. Nitrogen-use efficiency decreased with N levels but remained more or less similar under tillage and crop establishment practices. Maximum returns and B:C ratio were obtained under CT-flat, followed closely by ZT-flat.
THERE AND BACK AGAIN: RHETORICS OF AL-ANDALUS IN MODERN SYRIAN POPULAR CULTURE
Authors: Shannon Jonathan H.
Published: January 2016

This article explores the rhetorical function of al-Andalus (medieval Spain) in modern Syrian popular culture, with a focus on music. The rhetoric of al-Andalus in Syria is intimately related to the project of nation building. The nostalgic performance of links between modern Syria and medieval al-Andalus assumed great rhetorical force in the 1960s as a result of ideologies of pan-Arabism, the loss of Palestine, the rise of Islamist threats at home, and the emergence of petrodollar regimes in the Arabian Gulf. As a result, the rhetoric of al-Andalus became “good to think” for wide audiences of Syrians. Musical genres linked to al-Andalus play an important role as potent vehicles for constructing Syrian memory cultures. Drawing on heavily mythologized and nostalgic visions of an Andalusian golden age, musical performance in Syria sonically reinforces forms of nostalgic remembrance and enacts claims on Syrian pasts, presents, and futures.
TROUBLING THE POLITICAL: WOMEN IN THE JORDANIAN DAY-WAGED LABOR MOVEMENT
Authors: Ababneh Sara
Published: January 2016

The Jordanian Day-Waged Labor Movement (DWLM) played a central role in the Jordanian Popular Movement (al-Hirak al-Shaʿbi al-Urduni), commonly referred to as Hirak, from 2011 to the end of 2012. The large number of women who were active and took on leading roles in the DWLM contrasts with the absence of women's rights organizations in the Hirak. I argue that the DWLM was able to attract so many women because it developed a discourse and flexible structure that understood women to be embedded within communities and prioritized their economic needs. By studying this discourse and structure, it is possible to learn important lessons about gender-inclusive political and institutional reform.
Slow Activism: Listening to the Pain and Praise of Others
Authors: Kapchan Deborah
Published: January 2016

For what is needed is a philosophy of listening. But is this a possibility? If philosophy has its very roots intertwined with a secret vision of Being that has resulted in the present state of visualism, can it listen with equal profundity? What is called for is an ontology of the auditory.
Organizational Ecology and Institutional Change in Global Governance
Authors: Abbott Kenneth W. , Green Jessica F. , Keohane Robert O.
Published: January 2016

The institutions of global governance have changed dramatically in recent years. New organizational forms—including informal institutions, transgovernmental networks, and private transnational regulatory organizations (PTROs)—have expanded rapidly, while the growth of formal intergovernmental organizations has slowed. Organizational ecology provides an insightful framework for understanding these changing patterns of growth. Organizational ecology is primarily a structural theory, emphasizing the influence of institutional environments, especially their organizational density and resource availability, on organizational behavior and viability. To demonstrate the explanatory value of organizational ecology, we analyze the proliferation of PTROs compared with the relative stasis of intergovernmental organizations (IGOs). Continued growth of IGOs is constrained by crowding in their dense institutional environment, but PTROs benefit from organizational flexibility and low entry costs, which allow them to enter “niches” with limited resource competition. We probe the plausibility of our analysis by examining contemporary climate governance.
Genetic and infective diversity of the liver fluke Fasciola hepatica (Trematoda: Digenea) from Cuba
Authors: Vázquez A.A. , Lounnas M. , Sánchez J. , Alba A. , Milesi A. , Hurtrez-Boussès S.
Published: January 2016

In this study we present the first approach to exploration of the genetic diversity of Cuban Fasciola hepatica populations using microsatellite markers, coupled with observed prevalence in slaughterhouses. Nine populations of flukes recovered from cows and buffalos were studied in the central-western region of Cuba. The observed infection rates of definitive hosts (bovines) were 70–100% in most cases. An important amount of polymorphism was found in the four loci explored. However, no apparent genetic differences were found between populations from different provinces or bovine species. The absence of deviations from Hardy–Weinberg equilibrium suggests a high rate of cross-fertilization between F. hepatica individuals. This result was confirmed when all multilocus genotypes were tested for clonal reproduction and only four individuals differed statistically (Psex< 0.05). High values of expected heterozygosity coupled with highly probable mixing among strains make the metapopulation genetically diversified but similar in terms of certain alleles (low FST values). These results suggest a close relationship between parasite diversity and cattle management in Cuba. Our findings should be taken into consideration by veterinary authorities to help mitigate fasciolosis transmission.
New species and new records in the genusUsnea(Parmeliaceae, lichenized Ascomycota) from tropical South America
Authors: TRUONG Camille , CLERC Philippe
Published: January 2016

Usneais a cosmopolitan genus of lichen-forming Ascomycota (Parmeliaceae,Lecanorales). This hyperdiverse genus remains poorly known, especially in tropical regions, and recent efforts have been made to clarify the species concepts and describe the diversity of species. We describe five new species from tropical South America based on morphological and chemical features supported by molecular data from the ITS rDNA, nuLSU,RPB1andMcm7markers.Usnea clercianais a Galapagos endemic characterized by inflated branches holding elongated tubercles, excavate soralia and/or apothecia.Usnea araneaandU. subaraneashare inflated branches with numerous papillae and a loose arachnoid medulla, but differ in the minute and irregular soralia inU. aranea, that are circular and excavate inU. subaranea.Usnea rubriglabrataandU. subglabrataare characterized by inflated branches with excavate soralia, with a reddish cortex pigmentation inU. rubriglabratathat is lacking inU. subglabrata.Usnea aranea,U. rubriglabrata,U. subaraneaandU. subglabrataare so far endemic to continental South America; the three former species are closely related and cluster in a highly supported clade, characterized by species distributed in the tropical Andes. New records for the region are provided andU. morelianais resurrected, corresponding to the valid name forU. rubricornuta.
Immune activation and induction of memory: lessons learned from controlled human malaria infection with Plasmodium falciparum
Authors: SCHOLZEN ANJA , SAUERWEIN ROBERT W.
Published: January 2016

Controlled human malaria infections (CHMIs) are a powerful tool to assess the efficacy of drugs and/or vaccine candidates, but also to study anti-malarial immune responses at well-defined time points after infection. In this review, we discuss the insights that CHMI trials have provided into early immune activation and regulation during acute infection, and the capacity to induce and maintain immunological memory. Importantly, these studies show that a single infection is sufficient to induce long-lasting parasite-specific T- and B-cell memory responses, and suggest that blood-stage induced regulatory responses can limit inflammation both in ongoing and potentially future infections. As future perspective of investigation in CHMIs, we discuss the role of innate cell subsets, the interplay between innate and adaptive immune activation and the potential modulation of these responses after natural pre-exposure.
Regionalism and diffusion revisited: From final design towards stages of decision-making
Authors: Duina Francesco , Lenz Tobias
Published: January 2016

An emerging research programme on diffusion across regional international organisations (RIOs) proposes that decisions taken in one RIO affect decision-making in other RIOs. This work has provided a welcome corrective to endogenously-focused accounts of RIOs. Nevertheless, by focusing on the final design of policies and institutional arrangements, it has been conceptually overly narrow. This has led to a truncated understanding of diffusion’s impact and to an unjustified view of convergence as its primary outcome. Drawing on public policy and sociological research, we offer a conceptual framework that seeks to remedy these weaknesses by disaggregating the decision-making process on the ‘receiving’ side. We suggest that policies and institutional arrangements in RIOs result from three decision-making stages:problematisation(identification of something as a political problem),framing(categorisation of the problem and possible solutions), andscripting(design of final solutions). Diffusion can affectanycombination of these stages. Consequently, its effects are more varied and potentially extensive than is currently recognised, and convergence and persistent variation in scripting are both possible outcomes. We illustrate our framework by re-evaluating research on dispute settlement institutions in the EEC, NAFTA, and SADC. We conclude by discussing its theoretical implications and the conditions that likely promote diffusion.
Two-year-olds but not younger children comprehend it in ambiguous contexts: Evidence from preferential looking
Authors: SKARABELA BARBORA , OTA MITSUHIKO
Published: January 2016

Children use pronouns in their speech from the earliest word combinations. Yet, it is not clear from these early utterances whether they understand that pronouns are used as substitutes for nouns and entities in the discourse. The aim of this study was to examine whether young children understand the anaphoric function of pronouns, focusing on the interpretation of the pronoun it in English-speaking children at 1;6 and 2;0. We tested whether adults and children would prefer to look at a previously introduced vs. novel visual object depending on the argument form (it, the + noun, a + noun, or silence). Results demonstrate that, like adults, two-year-olds understand that it refers to a previously introduced referent. There is no evidence that this knowledge is established in children at 1;6. This suggests that some time between 1;6 and 2;0 children come to understand that it refers to a highly accessible referent introduced in the prior context.
Grassroots Transnationalism(s): Franco-German Opposition to Nuclear Energy in the 1970s
Authors: TOMPKINS ANDREW
Published: January 2016

During the 1970s opposition to nuclear energy was present in countries around the world and thus eminently ‘transnational’. But what did it mean to participate at the grassroots of such a transnational movement and (how) did cross-border connections change protest? This article answers these questions by differentiating three categories of transnational engagement that were accessible to grassroots activists. ‘Thinking transnationally’ involved extrapolating from, decontextualising and recontextualising limited information in order to rethink one's own situation. ‘Acting transnationally’ entailed accessing transnational spaces; it therefore required more mobility, but could be useful as a means of challenging and deconstructing state power. Intermediaries at the grassroots engaged in ‘being transnational’, which affected their personal and political identities as well as life histories. These examples of transnational agency illustrate how grassroots activists, including some without vast wealth or institutional resources, participated in transnational processes in ways that enriched, but also complicated protest.
The Friedrich Ebert Foundation and the Spanish Socialists during the Transition to Democracy, 1975–1982
Authors: MUÑOZ SÁNCHEZ ANTONIO
Published: January 2016

This article explores the activities of the Friedrich Ebert Foundation in Spain during the transition to democracy. It describes the financial, logistical and training support with which this German Foundation contributed to the unexpected rebirth of the Spanish Socialist Party after Franco and its meteoric emergence as the leading left-wing party. It also assesses its cooperation with the Socialist trade union, which moved from irrelevance to a position of importance greater than the powerful Communist union. Finally, the article examines how the Foundation diversified its activities in order to meet the growing needs of and challenges faced by the Spanish Socialists in their path towards power.
The Great Blank Space Beyond the Wall: Eastern Europe in Modern Intellectual History
Authors: ROBERTSON JAMES M.
Published: January 2016

Written seven years after the fall of the Berlin Wall, as the shockwaves of the Yugoslav wars still reverberated through the continent, Ugrešić’s essays spoke to a period of European history in which the West's lack of familiarity with the East was particularly marked. In part owing to this Western incomprehension, the period of the 1990s were a rich one for theoretical discussions within the field of Eastern European Studies, as scholars sharing Ugrešić’s concerns turned their attention to the ways in which Eastern Europe had come to be imagined as that great blank space beyond the wall.
The Inversion Number and the Major Index are Asymptotically Jointly Normally Distributed on Words
Authors: THIEL MARKO
Published: January 2016

In a recent paper, Baxter and Zeilberger showed that the two most important Mahonian statistics, the inversion number and the major index, are asymptotically independently normally distributed on permutations. In another recent paper, Canfield, Janson and Zeilberger proved the result, already known to statisticians, that the Mahonian distribution is asymptotically normal on words. This leaves one question unanswered: What, asymptotically, is the joint distribution of the inversion number and the major index on words? We answer this question by establishing convergence to a bivariate normal distribution.
Pathways for learning two languages: lexical and grammatical associations within and across languages in sequential bilingual children
Authors: PHAM GIANG
Published: January 2016

This study examines the strength and direction of lexical-grammatical associations within and between first and second languages (L1 and L2) in a longitudinal sample of sequential bilinguals. Thirty-three children who spoke Vietnamese (L1) and English (L2) completed picture-naming and story-telling tasks in each language at four yearly intervals. Hierarchical linear modeling across Years 1–4 revealed bidirectional within-language associations and a unidirectional cross-language association from the L1 to L2. Results suggest a conditional relationship between languages in which the L1 supports L2 growth, but not vice versa. Findings contribute to defining pathways for L1 and L2 learning across domains and languages.
OCEANS OF HISTORY, SEAS OF CHANGE: RECENT REVISIONIST WRITING IN WESTERN LANGUAGES ABOUT CHINA AND EAST ASIAN MARITIME HISTORY DURING THE PERIOD 1500–1630
Authors: Zurndorfer Harriet
Published: January 2016

This article focuses on recent revisionist scholarship demonstrating that China's maritime history in the period 1500 to 1630 is no longer a case of ‘missed opportunity’, a viewpoint fostered by earlier writing dominated by state-centric and land-focused models. To challenge this perspective, this study first reviews analyses demonstrating the far-reaching commercial networks between Ming China and localities in Southeast and Northeast Asia, and then considers the impact of the metaphor of Fernand Braudel's ‘Asian Mediterranean’ and his ideas about ‘world economy’ on the study of East Asian seafaring history. Secondly, this investigation reveals the dimensions of Chinese trade networks which the mid-Ming government officially sanctioned, as well as the extent to which literati from the southern provinces challenged the state's involvement in overseas commerce of trade and exchange. Finally, the article assesses how modern historians have studied late Ming maritime defense policies as security along the littoral lapsed.
THE QIAOBAN, THE PRC INFLUENCE AND NATIONALIST CHINESE IN THE NORTHERN THAI BORDERLAND
Authors: Siriphon Aranya
Published: January 2016

From 1989 onwards, the People's Republic of China's use of the Qiaoban, the Overseas Chinese Affairs Bureau, has burgeoned notably in regard to attracting investment and loyalty from overseas Chinese outside mainland China. This article analyses the impact of the Qiaoban and the PRC influence upon “diasporic Chinese” living along the northern Thai border, particularly ethnic Chinese (e.g. Teochiu, Hakka, Hainanese) and Nationalist Chinese(Kuomintang-KMT). It finds that, while the Qiaoban has largely succeeded in the engagement with the ethnic Chinese of northern Thailand, the active efforts of the PRC to engage the Nationalist Chinese have been notably unfruitful. However, the Qiaoban's efforts have caused increasing tension between different generations in Nationalist Chinese communities.
A social-ecological framework for analyzing and designing integrated crop–livestock systems from farm to territory levels
Authors: Moraine Marc , Duru Michel , Therond Olivier
Published: January 2016

Integrated crop–livestock systems are often considered a promising way to address agricultural sustainability issues. Many authors claim that complementarities and synergies between crops and livestock can improve nutrient cycling and delivery of ecosystem services (ES) in agricultural systems. They have analyzed effects of interactions at the farm level and affirmed the potential advantage of developing crop–livestock interactions at the territory level. However, potential benefits of developing synergies beyond the farm level have not been clearly identified. Thus, we developed a conceptual framework that can be used to analyze, design and perform integrated assessment of crop–livestock systems at the territory level. To address crop–livestock interaction issues, we define it as a social-ecological system called the territorial crop–livestock system (TCLS). The ecological system is represented as three interacting components, crops, grasslands and animals, allowing description of various land uses and their potential effects on nutrient cycling and ES. The social system, represented as farmers interacting with natural-resource managers and agro-food chain actors, determines land use and the nature and intensity of ES delivered. We highlight the importance of coordination and learning among actors to support implementation of complex adaptive systems such as TCLSs. We illustrate the expressive power of our conceptual framework through development of a generic typology of crop–livestock systems. Then we show how our conceptual framework can be used as an intermediary object with stakeholders in participatory design approaches. We illustrate this process by representing four archetypal TCLSs. We provide an example of the design approach implemented in Southwestern France to address severe recurrent water shortages, which includes analysis of land use in the current crop–livestock system and the associated key metabolic and ES issues, identification of options for change and multi-criteria analysis of these options. We conclude that this framework shows great potential to support development of sustainable farming systems at the territory level.
What Caused the Sudden Heating of Uranus's Atmosphere?
Authors: Wendel JoAnna
Published: January 2016

A recently observed temperature reversal on Uranus may offer a clue to a long-standing mystery: Why are the upper atmospheres of gas giants so hot?
Shaping the nonlinear near field
Authors: Wolf Daniela , Schumacher Thorsten , Lippitz Markus
Published: January 2016

Light scattering at plasmonic nanoparticles and their assemblies has led to a wealth of applications in metamaterials and nano-optics. Although shaping of fields around nanostructures is widely studied, the influence of the field inside the nanostructures is often overlooked. The linear field distribution inside the structure taken to the third power causes third-harmonic generation, a nonlinear optical response of matter. Here we demonstrate by a far field Fourier imaging method how this simple fact can be used to shape complex fields around a single particle alone. We employ this scheme to switch the third-harmonic emission from a single point source to two spatially separated but coherent sources, as in Young’s double-slit assembly. We envision applications as diverse as coherently feeding antenna arrays and optical spectroscopy of spatially extended electronic states.
Endogenous IL-6 of mesenchymal stem cell improves behavioral outcome of hypoxic-ischemic brain damage neonatal rats by supressing apoptosis in astrocyte
Authors: Gu Yan , He Mulan , Zhou Xiaoqin , Liu Jinngjing , Hou Nali , Bin Tan , Zhang Yun , Li Tingyu , Chen Jie
Published: January 2016

Mesenchymal stem cell (MSC) transplantation reduces the neurological impairment caused by hypoxic-ischemic brain damage (HIBD) via immunomodulation. In the current study, we found that MSC transplantation improved learning and memory function and enhanced long-term potentiation in neonatal rats subjected to HIBD and the amount of IL-6 released from MSCs was far greater than that of other cytokines. However, the neuroprotective effect of MSCs infected with siIL-6-transduced recombinant lentivirus (siIL-6 MSCs) was significantly weakened in the behavioural tests and electrophysiological analysis. Meanwhile, the hippocampal IL-6 levels were decreased following siIL-6 MSC transplantation. In vitro, the levels of IL-6 release and the levels of IL-6R and STAT3 expression were increased in both primary neurons and astrocytes subjected to oxygen and glucose deprivation (OGD) following MSCs co-culture. The anti-apoptotic protein Bcl-2 was upregulated and the pro-apoptotic protein Bax was downregulated in OGD-injured astrocytes co-cultured with MSCs. However, the siIL-6 MSCs suppressed ratio of Bcl-2/Bax in the injured astrocytes and induced apoptosis number of the injured astrocytes. Taken together, these data suggest that the neuroprotective effect of MSC transplantation in neonatal HIBD rats is partly mediated by IL-6 to enhance anti-apoptosis of injured astrocytes via the IL-6/STAT3 signaling pathway.
Two-dimensional Topological Crystalline Insulator Phase in Sb/Bi Planar Honeycomb with Tunable Dirac Gap
Authors: Hsu Chia-Hsiu , Huang Zhi-Quan , Crisostomo Christian P. , Yao Liang-Zi , Chuang Feng-Chuan , Liu Yu-Tzu , Wang Baokai , Hsu Chuang-Han , Lee Chi-Cheng , Lin Hsin , Bansil Arun
Published: January 2016

We predict planar Sb/Bi honeycomb to harbor a two-dimensional (2D) topological crystalline insulator (TCI) phase based on first-principles computations. Although buckled Sb and Bi honeycombs support 2D topological insulator (TI) phases, their structure becomes planar under tensile strain. The planar Sb/Bi honeycomb structure restores the mirror symmetry and is shown to exhibit non-zero mirror Chern numbers, indicating that the system can host topologically protected edge states. Our computations show that the electronic spectrum of a planar Sb/Bi nanoribbon with armchair or zigzag edges contains two Dirac cones within the band gap and an even number of edge bands crossing the Fermi level. Lattice constant of the planar Sb honeycomb is found to nearly match that of hexagonal-BN. The Sb nanoribbon on hexagonal-BN exhibits gapped edge states, which we show to be tunable by an out-of-the-plane electric field, providing controllable gating of edge state important for device applications.
A Search for Blood Biomarkers for Autism: Peptoids
Authors: Zaman Sayed , Yazdani Umar , Deng Yan , Li Wenhao , Gadad Bharathi S. , Hynan Linda , Karp David , Roatch Nichole , Schutte Claire , Nathan Marti C. , Hewitson Laura , German Dwight C.
Published: January 2016

Autism spectrum disorder (ASD) is a neurodevelopmental disorder characterized by impairments in social interaction and communication and restricted, repetitive patterns of behavior. In order to identify individuals with ASD and initiate interventions at the earliest possible age, biomarkers for the disorder are desirable. Research findings have identified widespread changes in the immune system in children with autism, at both systemic and cellular levels. In an attempt to find candidate antibody biomarkers for ASD, highly complex libraries of peptoids (oligo-N-substituted glycines) were screened for compounds that preferentially bind IgG from boys with ASD over typically developing (TD) boys. Unexpectedly, many peptoids were identified that preferentially bound IgG from TD boys. One of these peptoids was studied further and found to bind significantly higher levels (>2-fold) of the IgG1 subtype in serum from TD boys (n = 60) compared to ASD boys (n = 74), as well as compared to older adult males (n = 53). Together these data suggest that ASD boys have reduced levels (>50%) of an IgG1 antibody, which resembles the level found normally with advanced age. In this discovery study, the ASD1 peptoid was 66% accurate in predicting ASD.
Regulation of Asymmetric Division by Atypical Protein Kinase C Influences Early Specification of CD8+ T Lymphocyte Fates
Authors: Metz Patrick J. , Lopez Justine , Kim Stephanie H. , Akimoto Kazunori , Ohno Shigeo , Chang John T.
Published: January 2016

Naïve CD8+ T lymphocytes responding to microbial pathogens give rise to effector T cells that provide acute defense and memory T cells that provide long-lived immunity. Upon activation, CD8+ T lymphocytes can undergo asymmetric division, unequally distributing factors to the nascent daughter cells that influence their eventual fate towards the effector or memory lineages. Individual loss of either atypical protein kinase C (aPKC) isoform, PKCζ or PKCλ/ι, partially impairs asymmetric divisions and increases CD8+ T lymphocyte differentiation toward a long-lived effector fate at the expense of memory T cell formation. Here, we show that deletion of both aPKC isoforms resulted in a deficit in asymmetric divisions, increasing the proportion of daughter cells that inherit high amounts of effector fate-associated molecules, IL-2Rα, T-bet, IFNγR and interferon regulatory factor 4 (IRF4). However, unlike CD8+ T cells deficient in only one aPKC isoform, complete loss of aPKC unexpectedly increased CD8+ T cell differentiation toward a short-lived, terminal effector fate, as evidenced by increased rates of apoptosis and decreased expression of Eomes and Bcl2 early during the immune response. Together, these results provide evidence for an important role for asymmetric division in CD8+ T lymphocyte fate specification by regulating the balance between effector and memory precursors at the initiation of the adaptive immune response.
Wnt9a deficiency discloses a repressive role of Tcf7l2 on endocrine differentiation in the embryonic pancreas
Authors: Pujadas G. , Cervantes S. , Tutusaus A. , Ejarque M. , Sanchez L. , García A. , Esteban Y. , Fargas L. , Alsina B. , Hartmann C. , Gomis R. , Gasa R.
Published: January 2016

Transcriptional and signaling networks establish complex cross-regulatory interactions that drive cellular differentiation during development. Using microarrays we identified the gene encoding the ligand Wnt9a as a candidate target of Neurogenin3, a basic helix-loop-helix transcription factor that functions as a master regulator of pancreatic endocrine differentiation. Here we show that Wnt9a is expressed in the embryonic pancreas and that its deficiency enhances activation of the endocrine transcriptional program and increases the number of endocrine cells at birth. We identify the gene encoding the endocrine transcription factor Nkx2-2 as one of the most upregulated genes in Wnt9a-ablated pancreases and associate its activation to reduced expression of the Wnt effector Tcf7l2. Accordingly, in vitro studies confirm that Tcf7l2 represses activation of Nkx2-2 by Neurogenin3 and inhibits Nkx2-2 expression in differentiated β-cells. Further, we report that Tcf7l2 protein levels decline upon initiation of endocrine differentiation in vivo, disclosing the downregulation of this factor in the developing endocrine compartment. These findings highlight the notion that modulation of signalling cues by lineage-promoting factors is pivotal for controlling differentiation programs.
Focal Adhesion Kinase Regulates Fibroblast Migration via Integrin beta-1 and Plays a Central Role in Fibrosis
Authors: Zhao Xue-Ke , Cheng Yiju , Liang Cheng Ming , Yu Lei , Mu Mao , Li Hong , Liu Yang , Zhang Baofang , Yao Yumei , Guo Hui , Wang Rong , Zhang Quan
Published: January 2016

Lung fibrosis is a major medical problem for the aging population worldwide. Fibroblast migration plays an important role in fibrosis. Focal Adhesion Kinase (FAK) senses the extracellular stimuli and initiates signaling cascades that promote cell migration. This study first examined the dose and time responses of FAK activation in human lung fibroblasts treated with platelet derived growth factor BB (PDGF-BB). The data indicate that FAK is directly recruited by integrin β1 and the subsequent FAK activation is required for fibroblast migration on fibronectin. In addition, the study has identified that α5β1 and α4β1 are the major integrins for FAK-mediated fibroblast migration on fibronect. In contrast, integrins αvβ3, αvβ6 and αvβ8 play a minor but distinct role in fibroblast migration on fibronectin. FAK inhibitor significantly reduces PDGF-BB stimulated fibroblast migration. Importantly, FAK inhibitor protects bleomycin-induced lung fibrosis in mice. FAK inhibitor blocks FAK activation and significantly reduces signaling cascade of fibroblast migration in bleomycin-challenged mice. Furthermore, FAK inhibitor decreases lung fibrotic score, collagen accumulation, fibronectin production and myofibroblast differentiation in in bleomycin-challenged mice. These data demonstrate that FAK mediates fibroblast migration mainly via integrin β1. Furthermore, the findings suggest that targeting FAK signaling is an effective therapeutic strategy against fibrosis.
Angle Insensitive Color Filters in Transmission Covering the Visible Region
Authors: Mao Kening , Shen Weidong , Yang Chenying , Fang Xu , Yuan Wenjia , Zhang Yueguang , Liu Xu
Published: January 2016

Angle insensitive color filter based on Metal-SiOx-Metal structure is proposed in this paper, which can keep the same perceived transmitted color when the incidence angle changes from 0° to 60°, especially for p-polarization light. Various silicon oxide films deposited by reaction magnetron sputtering with a tunable refractive index from 1.97 to 3.84 is introduced to meet the strict angle insensitive resonance conditions. The angle resolved spectral filtering for both p-polarization light and s-polarization light are quite well, which can be attributed to the different physical origins for the high angular tolerance for two polarizations. Finally, the effect of SiOx absorption and Ag thickness on the peak transmittance are analyzed.
Benchmarking Deep Networks for Predicting Residue-Specific Quality of Individual Protein Models in CASP11
Authors: Liu Tong , Wang Yiheng , Eickholt Jesse , Wang Zheng
Published: January 2016

Quality assessment of a protein model is to predict the absolute or relative quality of a protein model using computational methods before the native structure is available. Single-model methods only need one model as input and can predict the absolute residue-specific quality of an individual model. Here, we have developed four novel single-model methods (Wang_deep_1, Wang_deep_2, Wang_deep_3 and Wang_SVM) based on stacked denoising autoencoders (SdAs) and support vector machines (SVMs). We evaluated these four methods along with six other methods participating in CASP11 at the global and local levels using Pearson’s correlation coefficients and ROC analysis. As for residue-specific quality assessment, our four methods achieved better performance than most of the six other CASP11 methods in distinguishing the reliably modeled residues from the unreliable measured by ROC analysis; and our SdA-based method Wang_deep_1 has achieved the highest accuracy, 0.77, compared to SVM-based methods and our ensemble of an SVM and SdAs. However, we found that Wang_deep_2 and Wang_deep_3, both based on an ensemble of multiple SdAs and an SVM, performed slightly better than Wang_deep_1 in terms of ROC analysis, indicating that integrating an SVM with deep networks works well in terms of certain measurements.
Reproducibility of Intra- and Inter-scanner Measurements of Liver Fat Using Complex Confounder-corrected Chemical Shift Encoded MRI at 3.0 Tesla
Authors: Wu Bing , Han Wei , Li Zhenhong , Zhao Yonghua , Ge Mingmei , Guo Xueqing , Wu Xinhuai
Published: January 2016

The purpose of this study was to prospectively evaluate the reproducibility of the proton density fat-fraction (PDFF) of the liver using the IDEAL algorithm, a quantitative confounder-corrected chemical-shift-encoded MRI method. Data were obtained from 15 volunteers on four different days. The first and the third examinations were conducted on scanner one with one-week intervals, while the second and the fourth tests were performed on scanner two with same time interval. For each test, two MR scans were performed, one before and one after a meal. Regions-of-interest measurements were manually calculated to estimate the PDFF in the right and left lobes on the PDFF images. Reproducibility was measured using the intra-class correlation coefficient (ICC). The ICCs of the PDFF in the right and left lobes were 0.935 and 0.878, respectively. The intra-scanner ICCs of the right lobe before and after a meal or at a one-week interval were 0.924 and 0.953, respectively. The inter-scanner ICCs of PDFF the next day and at a one-week interval were 0.920 and 0.864, respectively. The PDFF of liver derived from IDEAL demonstrated high intra- and inter-scanner measurement reproducibility. The PDFF of the right lobe before a meal was more reproducible than after-meal measurements.
Matrix-Assisted Plasma Atomization Emission Spectrometry for Surface Sampling Elemental Analysis
Authors: Yuan Xin , Zhan Xuefang , Li Xuemei , Zhao Zhongjun , Duan Yixiang
Published: January 2016

An innovative technology has been developed involving a simple and sensitive optical spectrometric method termed matrix-assisted plasma atomization emission spectrometry (MAPAES) for surface sampling elemental analysis using a piece of filter paper (FP) for sample introduction. MAPAES was carried out by direct interaction of the plasma tail plume with the matrix surface. The FP absorbs energy from the plasma source and releases combustion heating to the analytes originally present on its surface, thus to promote the atomization and excitation process. The matrix-assisted plasma atomization excitation phenomenon was observed for multiple elements. The FP matrix served as the partial energy producer and also the sample substrate to adsorb sample solution. Qualitative and quantitative determinations of metal ions were achieved by atomic emission measurements for elements Ba, Cu, Eu, In, Mn, Ni, Rh and Y. The detection limits were down to pg level with linear correlation coefficients better than 0.99. The proposed MAPAES provides a new way for atomic spectrometry which offers advantages of fast analysis speed, little sample consumption, less sample pretreatment, small size and cost-effective.
Thermally triggered phononic gaps in liquids at THz scale
Authors: Bolmatov Dima , Zhernenkov Mikhail , Zav’yalov Dmitry , Stoupin Stanislav , Cunsolo Alessandro , Cai Yong Q.
Published: January 2016

In this paper we present inelastic X-ray scattering experiments in a diamond anvil cell and molecular dynamic simulations to investigate the behavior of phononic excitations in liquid Ar. The spectra calculated using molecular dynamics were found to be in a good agreement with the experimental data. Furthermore, we observe that, upon temperature increases, a low-frequency transverse phononic gap emerges while high-frequency propagating modes become evanescent at the THz scale. The effect of strong localization of a longitudinal phononic mode in the supercritical phase is observed for the first time. The evidence for the high-frequency transverse phononic gap due to the transition from an oscillatory to a ballistic dynamic regimes of motion is presented and supported by molecular dynamics simulations. This transition takes place across the Frenkel line thermodynamic limit which demarcates compressed liquid and non-compressed fluid domains on the phase diagram and is supported by calculations within the Green-Kubo phenomenological formalism. These results are crucial to advance the development of novel terahertz thermal devices, phononic lenses, mirrors and other THz metamaterials.
Mapping of Low-Frequency Raman Modes in CVD-Grown Transition Metal Dichalcogenides: Layer Number, Stacking Orientation and Resonant Effects
Authors: O’Brien Maria , McEvoy Niall , Hanlon Damien , Hallam Toby , Coleman Jonathan N. , Duesberg Georg S.
Published: January 2016

Layered inorganic materials, such as the transition metal dichalcogenides (TMDs), have attracted much attention due to their exceptional electronic and optical properties. Reliable synthesis and characterization of these materials must be developed if these properties are to be exploited. Herein, we present low-frequency Raman analysis of MoS2, MoSe2, WSe2 and WS2 grown by chemical vapour deposition (CVD). Raman spectra are acquired over large areas allowing changes in the position and intensity of the shear and layer-breathing modes to be visualized in maps. This allows detailed characterization of mono- and few-layered TMDs which is complementary to well-established (high-frequency) Raman and photoluminescence spectroscopy. This study presents a major stepping stone in fundamental understanding of layered materials as mapping the low-frequency modes allows the quality, symmetry, stacking configuration and layer number of 2D materials to be probed over large areas. In addition, we report on anomalous resonance effects in the low-frequency region of the WS2 Raman spectrum.