bluuebunny/crossref_metadata_2025 huggingface dataset:
Intronic TP53 Polymorphisms Are Associated with Increased Δ133TP53 Transcript, Immune Infiltration and Cancer Risk
Authors: Eiholzer Ramona A. , Mehta Sunali , Kazantseva Marina , Drummond Catherine J. , McKinney Cushla , Young Katie , Slater David , Morten Brianna C. , Avery-Kiejda Kelly A. , Lasham Annette , Fleming Nicholas , Morrin Helen R. , Reader Karen , Royds Janice A. , Landmann Michael , Petrich Simone , Reddel Roger , Huschtscha Lily , Taha Ahmad , Hung Noelyn A. , Slatter Tania L. , Braithwaite Antony W.
Published: September 2020

We investigated the influence of selected TP53 SNPs in exon 4 and intron 4 on cancer risk, clinicopathological features and expression of TP53 isoforms. The intron 4 SNPs were significantly over-represented in cohorts of mixed cancers compared to three ethnically matched controls, suggesting they confer increased cancer risk. Further analysis showed that heterozygosity at rs1042522(GC) and either of the two intronic SNPs rs9895829(TC) and rs2909430(AG) confer a 2.34–5.35-fold greater risk of developing cancer. These SNP combinations were found to be associated with shorter patient survival for glioblastoma and prostate cancer. Additionally, these SNPs were associated with tumor-promoting inflammation as evidenced by high levels of infiltrating immune cells and expression of the Δ133TP53 and TP53β transcripts. We propose that these SNP combinations allow increased expression of the Δ133p53 isoforms to promote the recruitment of immune cells that create an immunosuppressive environment leading to cancer progression.
ATP Production Relies on Fatty Acid Oxidation Rather than Glycolysis in Pancreatic Ductal Adenocarcinoma
Authors: Lee Jae-Seon , Oh Su-Jin , Choi Hyun-Jung , Kang Joon Hee , Lee Seon-Hyeong , Ha Ji Sun , Woo Sang Myung , Jang Hyonchol , Lee Ho , Kim Soo-Youl
Published: September 2020

Glycolysis is known as the main pathway for ATP production in cancer cells. However, in cancer cells, glucose deprivation for 24 h does not reduce ATP levels, whereas it does suppress lactate production. In this study, metabolic pathways were blocked to identify the main pathway of ATP production in pancreatic ductal adenocarcinoma (PDAC). Blocking fatty acid oxidation (FAO) decreased ATP production by 40% in cancer cells with no effect on normal cells. The effects of calorie balanced high- or low-fat diets were tested to determine whether cancer growth is modulated by fatty acids instead of calories. A low-fat diet caused a 70% decrease in pancreatic preneoplastic lesions compared with the control, whereas a high-fat diet caused a two-fold increase in preneoplastic lesions accompanied with increase of ATP production in the Kras (G12D)/Pdx1-cre PDAC model. The present results suggest that ATP production in cancer cells is dependent on FAO rather than on glycolysis, which can be a therapeutic approach by targeting cancer energy metabolism.
Genotype of Null Polymorphisms in Genes GSTM1, GSTT1, CYP1A1, and CYP1A1*2A (rs4646903 T>C)/CYP1A1*2C (rs1048943 A>G) in Patients with Larynx Cancer in Southeast Spain
Authors: Sánchez-Siles Mariano , Pelegrín-Hernández Juan Pablo , Hellin-Meseguer Diego , Guerrero-Sánchez Yolanda , Corno-Caparrós Andrés , Cabezas-Herrera Juan , Pastor-Quirante Francisco , Fernández-Ruiz Juan Alberto , Aliaga-Sánchez Alfonso , Lucero-Berdugo Mayra , Camacho-Alonso Fabio
Published: September 2020

Background: some types of cancer have been associated with the presence of single nucleotide polymorphisms (SNPs) of some genes that encode enzymes: glutathione-S transferase (GST), whose alteration leads to loss of function and a lower capacity to eliminate toxic GSTM1 and GSTT1 null genotypes; SNPs causing loss of function of CYP1A1 or CYP1A1–2 cytochrome P450 enzymes related with a lower capacity to deactivate hydrocarbons related to smoking, which involves a higher risk of developing some smoking-dependent cancers including larynx cancer. Objective: to compare the presence of null SNPs in genes GSTM1, GSTT1, and CYP1A1 rs 4646903 T>C, and CYP1A1–2 RS1048943 A>G in patients with hypopharyngeal and larynx cancer with a healthy control group. Materials and method: The study included a total of 80 patients with hypopharyngeal and laryngeal cancer and 23 healthy subjects. Genomic DNA was obtained from saliva samples, determining genotype GSTM1 (present +, or null −), GSTT1 (present + or null −). Polymorphisms (SNP) in CYP1A1 T>C (present + CC, or absent − TC/TT), and CYP1A1–2 A>G (present + GG, or absent − AG/AA). Results: the mean age of patients with larynx cancer was 62 years and of control subjects 63 years. Of the total sample, over 95% were men, and over 90% were smokers. The presence of null genotypes for GTM1 was 50% in patients with larynx cancer (p = 0.042), while GSTT1 was 88.75% (p = 0.002). CYP1A1 rs4646903 T>C polymorphisms were detected in 100% of cases of larynx cancer and 17.39% of healthy subjects (p > 0.001). Conclusions: patients with larynx cancer present more gene GSTM1 and GSTT1 null polymorphisms, and CYP1A1 rs4646903 T>C polymorphisms.
Targeting Signaling Pathways in Inflammatory Breast Cancer
Authors: Wang Xiaoping , Semba Takashi , Phi Lan Thi Hanh , Chainitikun Sudpreeda , Iwase Toshiaki , Lim Bora , Ueno Naoto T.
Published: September 2020

Inflammatory breast cancer (IBC), although rare, is the most aggressive type of breast cancer. Only 2–4% of breast cancer cases are classified as IBC, but—owing to its high rate of metastasis and poor prognosis—8% to 10% of breast cancer-related mortality occur in patients with IBC. Currently, IBC-specific targeted therapies are not available, and there is a critical need for novel therapies derived via understanding novel targets. In this review, we summarize the biological functions of critical signaling pathways in the progression of IBC and the preclinical and clinical studies of targeting these pathways in IBC. We also discuss studies of crosstalk between several signaling pathways and the IBC tumor microenvironment.
3D Technology Development and Dental Education: What Topics Are Best Suited for 3D Learning Resources?
Authors: Poblete Paulina , McAleer Sean , Mason Andrew G
Published: September 2020

The aim of this study is to identify topics (knowledge and skills) from the dental curricula that would benefit from having a 3D learning resource using an exploratory sequential design method. The first phase targeted stakeholders from a Scottish dental school. Seven focus groups and three interviews disclosed 97 suitable topics for 3D technology development. These results were used to construct a survey that was sent to final year dental students, newly dental graduates and academics from three Scottish universities. The survey asked participants to rank each item based on the perceived benefit that a 3D learning resource would have for dental education. Results revealed that detailed anatomy of the temporomandibular joint, dental anaesthesiology, dental clinical skills techniques, dental occlusion and mandibular functioning were top priorities. Gender differences only appeared in relation to ‘Extraction techniques: movements and force’ (p < 0.05), this topic was considered to be more beneficial by females than by males. No statistical difference was found when comparing results of graduates with undergraduates. These results serve as a starting point when developing a new 3D technology tool for dental education, considering users demands and perceived needs has the potential to benefit dental students and dental education directly.
Artificial Neural Network for the Thermal Comfort Index Prediction: Development of a New Simplified Algorithm
Authors: Palladino Domenico , Nardi Iole , Buratti Cinzia
Published: September 2020

A simplified algorithm using an artificial neural network (ANN, a feed-forward neural network) for the assessment of the predicted mean vote (PMV) index in summertime was developed, using solely three input variables (namely the indoor air temperature, relative humidity, and clothing insulation), whilst low air speed (<0.1 m/s), a minimal variation of radiant temperature (25.1 °C ± 2 °C) and steady metabolism (1.2 Met) were considered. Sensitivity analysis to the number of variables and to the number of neurons were performed. The developed ANN was then compared with three proven methods used for thermal comfort prediction: (i) the International Standard; (ii) the Rohles model; (iii) the modified Rohles model. Finally, another network able to predict the indoor thermal conditions was considered: the combined calculation of the two networks was tested for the PMV prediction. The proposed algorithm allows one to better approximate the PMV index than the other models (mean error of ANN predominantly in ±0.10–±0.20 range). The accuracy of the network in PMV prediction increases when air temperature and relative humidity values fall into 21–28 °C and 30–75% ranges. When the PMV is predicted by using the combined calculation (i.e., by using the two networks), the same order of magnitude of error was found, confirming the reliability of the networks. The developed ANN could be considered as an alternative method for the simplified prediction of PMV; moreover, the new simplified algorithm can be useful in buildings’ design phase, i.e., in those cases where experimental data are not available.
Intraday Electricity Pricing of Night Contracts
Authors: Kremer Marcel , Kiesel Rüdiger , Paraschiv Florentina
Published: September 2020

This paper investigates the intraday electricity pricing of 15-min. contracts in night hours. We tailor a recently introduced econometric model with fundamental impacts, which is successful in describing the pricing of day contracts. Our estimation results show that the mean reversion and the positive price impact of neighboring contracts are generic features of the price formation process on the intraday market, independent of the time of day. Intraday auction prices have higher explanatory power for the pricing of night than day contracts, particularly, for the first and last 15-min. contract in a night hour. Intradaily updated forecasts of wind power infeed are the only significant fundamental factors for intraday electricity prices at night. Neither expected conventional capacities nor the slope of the merit order curve contribute to explaining price dynamics. Overall, we conclude that fundamentals lose in importance in night hours and the 15-min. intraday market is rather driven by price information.
An Analog Magnetic Isolator for Space Power Applications
Authors: F. Miaja Pablo , Lopez Abraham , Arias Manuel
Published: September 2020

In power electronic applications, often a galvanic isolation barrier has to be passed. An example of this is the feedback control of isolated DC/DC converters where the secondary voltage has to be passed to the primary side where the control circuitry lies. For these applications, the use of optocouplers is well known in terrestrial applications. However, optocouplers tend to suffer degradation from the radiation damage induced by the space environment. For this reason, some space grade DC/DC converters use some form of magnetic feedback. In this paper, a magnetic analog isolator is presented. It will allow for passing an analog voltage through an isolation barrier by means of a magnetic transformer based circuit. It will be based on an LLC DC/DC converter so the gain between both sides of the isolator can be tuned. Design methodology will be presented so the circuit can be tailored for any foreseen application. In this paper, a simulation of its application in a Latching Current Limiter is presented. All the components will be discrete components which have a rad-hard version. A design example having a bandwidth around 20 kHz and a gain of 4.6 dB is shown in the Experimental Results sections to support the analysis.
Coloured BIPV Technologies: Methodological and Experimental Assessment for Architecturally Sensitive Areas
Authors: Pelle Martina , Lucchi Elena , Maturi Laura , Astigarraga Alexander , Causone Francesco
Published: September 2020

Energy flexibility in buildings is gaining momentum with the introduction of new European directives that enable buildings to manage their own energy demand and production, by storing, consuming or selling electricity according to their need. The transition towards a low-carbon energy system, through the promotion of on-site energy production and enhancement of self-consumption, can be supported by building-integrated photovoltaics (BIPV) technologies. This paper investigates the aesthetic and technological integration of hidden coloured PV modules in architecturally sensitive areas that seem to be the best possibility to favour a balance between conservation and energy issues. First, a multidisciplinary methodology for evaluating the aesthetic and technical integration of PV systems in architecturally sensitive area is proposed, referring to the technologies available on the market. Second, the experimental characterisation of the technical performance specific BIPV modules and their comparison with standard modules under standard weather condition are analysed, with the aim of acquiring useful data for comparing the modules’ integration properties and performance. For this purpose, new testbeds have been set up to investigate the aesthetic integration and the energy performances of innovative BIPV products. The paper describes the analyses carried out to define the final configuration of these experimental testbeds. Finally, the experimental characterisation at standard test conditions of two coloured BIPV modules is presented and the experimental design for the outdoor testing is outlined.
Redox Flow Batteries: A Literature Review Oriented to Automatic Control
Authors: Clemente Alejandro , Costa-Castelló Ramon
Published: September 2020

This paper presents a literature review about the concept of redox flow batteries and its automation and monitoring. Specifically, it is focused on the presentation of all-vanadium redox flow batteries which have several benefits, compared with other existing technologies and methods for energy stored purposes. The main aspects that are reviewed in this work correspond to the characterization, modeling, supervision and control of the vanadium redox flow batteries. A research is presented where redox flow batteries are contextualized in the current energy situation, compared with other types of energy storage systems. Furthermore, a presentation about the current challenges on research, and the main existing installations is view. A discussion about the main dynamic models that have been proposed during last years, as well as the different control strategies and observers, is presented.
Sizing of Lithium-Ion Battery/Supercapacitor Hybrid Energy Storage System for Forklift Vehicle
Authors: Paul Théophile , Mesbahi Tedjani , Durand Sylvain , Flieller Damien , Uhring Wilfried
Published: September 2020

Nowadays, electric vehicles are one of the main topics in the new industrial revolution, called Industry 4.0. The transport and logistic solutions based on E-mobility, such as handling machines, are increasing in factories. Thus, electric forklifts are mostly used because no greenhouse gas is emitted when operating. However, they are usually equipped with lead-acid batteries which present bad performances and long charging time. Therefore, combining high-energy density lithium-ion batteries and high-power density supercapacitors as a hybrid energy storage system results in almost optimal performances and improves battery lifespan. The suggested solution is well suited for forklifts which continuously start, stop, lift up and lower down heavy loads. This paper presents the sizing of a lithium-ion battery/supercapacitor hybrid energy storage system for a forklift vehicle, using the normalized Verein Deutscher Ingenieure (VDI) drive cycle. To evaluate the performance of the lithium-ion battery/supercapacitor hybrid energy storage system, different sizing simulations are carried out. The suggested solution allows us to successfully optimize the system in terms of efficiency, volume and mass, in regard to the battery, supercapacitors technology and the energy management strategy chosen.
Electro-Hydraulic Transient Regimes in Isolated Pumps Working as Turbines with Self-Excited Induction Generators
Authors: Madeira Filipe C. , Fernandes João F. P. , Pérez-Sánchez Modesto , López-Jiménez P. Amparo , Ramos Helena M. , Costa Branco P. J.
Published: September 2020

The use of pumps working as turbines (PATs) is a sustainable technical measure that contributes to the improvement of energy efficiency in water systems. However, its performance analysis in off-grid recovery systems is a complex task that must consider both hydraulic (PAT) and electrical machines (typically a self-excited induction generator-SEIG). Aside from several kinds of research that analyze the PAT-SEIG behavior under steady-state constant hydraulic and electrical conditions, this research focuses on the analysis of PAT-SEIG transient regimes, by analyzing their variation when a sudden change occurs in the hydraulic or electrical components. Analytical models were developed to represent the operation of SEIG, PAT, and the PAT-SEIG coupled system. Hydraulic and electromechanical experimental tests validated these models. An excellent fit was obtained when analytical and experimental values were compared. With these models, the impact on the operation of the PAT-SEIG system was examined when sudden change occurred in the excitation capacitances, resistive loads, or recovered head. With a sudden increase of resistive load, the hydraulic power and SEIG stator current remain almost constant. However, there is an increase of SEIG reactive power, decreasing the PAT-SEIG efficiency. Also, with a sudden increase of SEIG capacitors or PAT hydraulic head, the SEIG stator current increases once and not again, while PAT-SEIG efficiency decreases, but the induction generator can be overloaded. The development of this research is key to the advancement of future models which can analyze the coupling of micro-hydropower solutions.
Effect of Microwave Processing and Glass Inclusions on Thermoelectric Properties of P-Type Bismuth Antimony Telluride Alloys for Wearable Applications
Authors: Nozariasbmarz Amin , Vashaee Daryoosh
Published: September 2020

Depending on the application of bismuth telluride thermoelectric materials in cooling, waste heat recovery, or wearable electronics, their material properties, and geometrical dimensions should be designed to optimize their performance. Recently, thermoelectric materials have gained a lot of interest in wearable electronic devices for body heat harvesting and cooling purposes. For efficient wearable electronic devices, thermoelectric materials with optimum properties, i.e., low thermal conductivity, high Seebeck coefficient, and high thermoelectric figure-of-merit (zT) at room temperature, are demanded. In this paper, we investigate the effect of glass inclusion, microwave processing, and annealing on the synthesis of high-performance p-type (BixSb1−x)2Te3 nanocomposites, optimized specially for body heat harvesting and body cooling applications. Our results show that glass inclusion could enhance the room temperature Seebeck coefficient by more than 10% while maintaining zT the same. Moreover, the combination of microwave radiation and post-annealing enables a 25% enhancement of zT at room temperature. A thermoelectric generator wristband, made of the developed materials, generates 300 μW power and 323 mV voltage when connected to the human body. Consequently, MW processing provides a new and effective way of synthesizing p-type (BixSb1−x)2Te3 alloys with optimum transport properties.
Synergy of Thermochemical Treatment of Dried Distillers Grains with Solubles with Bioethanol Production for Increased Sustainability and Profitability
Authors: O’Brien Samuel , Koziel Jacek A. , Banik Chumki , Białowiec Andrzej
Published: September 2020

The bioethanol industry continues improving sustainability, specifically focused on plant energy and GHG emission management. Dried distiller grains with solubles (DDGS) is a byproduct of ethanol fermentation and is used for animal feed. DDGS is a relatively low-value bulk product that decays, causes odor, and is challenging to manage. The aim of this research was to find an alternative, value-added-type concept for DDGS utilization. Specifically, we aimed to explore the techno-economic feasibility of torrefaction, i.e., a thermochemical treatment of DDGS requiring low energy input, less sophisticated equipment, and resulting in fuel-quality biochar. Therefore, we developed a research model that addresses both bioethanol production sustainability and profitability due to synergy with the torrefaction of DDGS and using produced biochar as marketable fuel for the plant. Our experiments showed that DDGS-based biochar (CSF—carbonized solid fuel) lower calorific value may reach up to 27 MJ∙kg−1 d.m. (dry matter) Specific research questions addressed were: What monetary profits and operational cost reductions could be expected from valorizing DDGS as a source of marketable biorenewable energy, which may be used for bioethanol production plant’s demand? What environmental and financial benefits could be expected from valorizing DDGS to biochar and its reuse for natural gas substitution? Modeling indicated that the valorized CSF could be produced and used as a source of energy for the bioethanol production plant. The use of heat generated from CSF incineration supplies the entire heat demand of the torrefaction unit and the heat demand of bioethanol production (15–30% of the mass of CSF and depending on the lower heating value (LHV) of the CSF produced). The excess of 70–85% of the CSF produced has the potential to be marketed for energetic, agricultural, and other applications. Preliminary results show the relationship between the reduction of the environmental footprint (~24% reduction in CO2 emissions) with the introduction of comprehensive on-site valorization of DDGS. The application of DDGS torrefaction and CSF recycling may be a source of the new, more valuable revenues and bring new perspectives to the bioethanol industry to be more sustainable and profitable, including during the COVID-19 pandemic and other shocks to market conditions.
An Accurate State of Charge Estimation Method for Lithium Iron Phosphate Battery Using a Combination of an Unscented Kalman Filter and a Particle Filter
Authors: Nguyen Thanh-Tung , Khan Abdul Basit , Ko Younghwi , Choi Woojin
Published: September 2020

An accurate state of charge (SOC) estimation of the battery is one of the most important techniques in battery-based power systems, such as electric vehicles (EVs) and energy storage systems (ESSs). The Kalman filter is a preferred algorithm in estimating the SOC of the battery due to the capability of including the time-varying coefficients in the model and its superior performance in the SOC estimation. However, since its performance highly depends on the measurement noise (MN) and process noise (PN) values, it is difficult to obtain highly accurate estimation results with the battery having a flat plateau OCV (open-circuit voltage) area in the SOC-OCV curve, such as the Lithium iron phosphate battery. In this paper, a new integrated estimation method is proposed by combining an unscented Kalman filter and a particle filter (UKF-PF) to estimate the SOC of the Lithium iron phosphate battery. The equivalent circuit of the battery used is composed of a series resistor and two R-C parallel circuits. Then, it is modeled by a second-order autoregressive exogenous (ARX) model, and the parameters are identified by using the recursive least square (RLS) identification method. The validity of the proposed algorithm is verified by comparing the experimental results obtained with the proposed method and the conventional methods.
Both Mature Patches and Expanding Areas of Juniperus thurifera Forests Are Vulnerable to Climate Change But for Different Reasons
Authors: Acuña-Míguez Belén , Valladares Fernando , Martín-Forés Irene
Published: September 2020

Research Highlights: Water use efficiency (WUE) varied along a gradient of Juniperus thurifera (L.) forest expansion, being higher in recently colonised areas. Background and Objectives: WUE is a classic physiological process of plants that reflects the compromise between carbon assimilation and water loss and has a profound influence on their performance in water-limited environments. Forest expansion in Mediterranean regions associated with land abandonment can influence the WUE of plants due to the existence of two opposing gradients: one of favourable–unfavourable environmental conditions and another one of increased–decreased intraspecific competition, the former increasing and the latter decreasing towards the expanding front. The main objective of this study was to elucidate how the WUE of Juniperus thurifera varied along the stages of forest expansion and to provide insight on how this variation is influenced by intraspecific competition and abiotic factors. Materials and Methods: Seventeen plots at different distances from the mature forest core were selected at three sites located in the centre of the Iberian Peninsula. For 30 individuals within each plot, we measured biometric characteristics, age, tree vigour, and C/N ratio in leaves, and the leaf carbon isotope signature (δ13C (‰)) as a proxy for WUE. Around each individual, we scored the percentage cover of bare soil, stoniness, conspecifics, and other woody species. Results: WUE of J. thurifera individuals varied along the forest expansion gradient, being greater for the individuals at the expanding front than for those at the mature forest. WUE was influenced by the cover of conspecifics, tree age, and C/N ratio in leaves. This pattern reveals that less favourable environmental conditions (i.e., rocky soils and higher radiation due to lower vegetation cover) and younger trees at the expanding front are associated with increased WUE. The increased cover of conspecifics decreases irradiance at the mature forest, involving milder stress conditions than at the expanding front. Conclusions: Lower WUE in mature forests due to more favourable conditions and higher WUE due to abiotic stress at expanding fronts revealed high constraints on water economy of this tree species in these two contrasting situations. Climate change scenarios bringing increased aridity are a serious threat to Juniperus thurifera forests, affecting both mature and juvenile populations although in different ways, which deserve further research to fully unveil.
New Generalized Viscosity Model for Non-Colloidal Suspensions and Emulsions
Authors: Pal Rajinder
Published: September 2020

The viscous behavior of solids-in-liquid suspensions and liquid-in-liquid emulsions of non-Brownian solid particles and liquid droplets dispersed in Newtonian liquids is thoroughly discussed and reviewed. The full concentration range of the dispersed particles/droplets is covered, that is, 0<ϕ<ϕm, where ϕ is the volume fraction of inclusions (particles or droplets) and ϕm is the maximum packing volume fraction of inclusions. The existing viscosity models for suspensions and emulsions are evaluated using a large pool of experimental viscosity data on suspensions and emulsions. A new generalized model for the viscosity of suspensions and emulsions is proposed and evaluated. The model takes into consideration the influence of shear-induced aggregation of particles and droplets. It also includes the effect of the droplet-to-matrix viscosity ratio λ on the viscosity of emulsions. In the limit of high ratio of droplet viscosity to matrix viscosity (λ→∞), the model reduces to the suspension viscosity model. The proposed model uncovers some important and novel characteristics of suspension systems rarely discussed heretofore in the literature. The model is validated using twenty sets of experimental viscosity data on solids-in-liquid suspensions and twenty-three sets of experimental viscosity data on liquid-in-liquid emulsions.
Shame and Theory-of-Mind Predicts Rule-Following Behavior
Authors: Ridinger Garret
Published: September 2020

This paper examines the idea that adherence to social rules is in part driven by moral emotions and the ability to recognize the emotions of others. Moral emotions like shame and guilt produce negative feelings when social rules are transgressed. The ability to recognize and understand the emotions of others is known as affective theory of mind (ToM). ToM is necessary for people to understand how others are affected by the violations of social rules. Using a laboratory experiment, individuals participated in a rule-following task designed to capture the propensity to follow costly social rules and completed psychometric measures of guilt, shame, and ToM. The results show that individuals who feel more shame and have higher ToM are more likely to follow the rules. The results from this experiment suggest that both shame and ToM are important in understanding rule-following.
The Effect of Positive Emotion and Interpersonal Relationships to Adaptation of School Life on High School Athletic Class Students
Authors: Chang Chia-Fu , Hsieh Huey-Hong , Huang Hsiu-Chin , Huang Yu-Lan
Published: September 2020

Background: Adaption for school life is important for all students. As for athletic students, since they need to cope with schoolwork and extensive training, adaption for school life could be very challenging. Taking this into consideration, the purpose of this study was to explore the factors which may help high school athletic students’ adaption of school life. Owing to this, the study explored previous researches and proposed four hypotheses: the first two hypotheses proposed that athletes’ positive emotion will have positive impacts on both their interpersonal relationships and adaption of school life; the third hypothesis suggests that athletes’ interpersonal relationships will have positive impacts on their adaption of school life and the fourth hypothesis suggested that interpersonal relationships play a mediating role among the positive emotion’s effect on adaption of school life. Methods: A total of 800 structured questionnaires were distributed to eleven high schools with athletic class students for data collection with a valid return rate of 90.6%. Structural equation modelling was used to test the relationship among them. Results: The result showed that positive emotion (β = 0.72, p < 0.05) and interpersonal relationships (β = 0.34, p < 0.05) had positive impacts on students’ adaption of school life with a predictive power of 68%. In addition, positive emotion also affected students’ school life adaption through interpersonal relationships. Conclusion: The study confirmed the positive emotion can have significant influences on student athletes’ interpersonal relationships and school life adaption. Implications: According to our findings, we suggest to encourage and promote athletes’ positive emotions so to help them have better interpersonal relationships and school life adaption.
Perceived Severity and Susceptibility towards Leptospirosis Infection in Malaysia
Authors: Sukeri Surianti , Zahiruddin Wan Mohd , Shafei Mohd Nazri , Hamat Rukman Awang , Osman Malina , Jamaluddin Tengku Zetty Maztura Tengku , Daud Aziah Binti
Published: September 2020

Background: Perceived severity and susceptibility refers to one’s belief of the seriousness and the risk of contracting a specific disease. It is an essential study in public health as it assists in the understanding of the motivating factors towards disease prevention. This qualitative study aimed to explore perceived severity and susceptibility towards leptospirosis infection among respondents in two states of Malaysia. Methods: Focus group discussions using the phenomenology approach was conducted involving 72 respondents in Selangor and Kelantan. Data were examined using content analysis. Results: Respondents perceived leptospirosis infection as severe due to its poor disease prognosis and complications. However, some rated it less severe when compared with other chronic diseases such as cancer and AIDS. Their perceptions were influenced by their knowledge about the disease, media portrayal and frequency of health campaigns by the government. All respondents believed they were not susceptible to the disease. Conclusion: The low perceived susceptibility of leptospirosis infection is a matter of concern as it may contribute to respondents’ lack of motivation towards preventing the disease. The study findings may provide the basis for health promotional activities designed to heighten public perceived threat towards leptospirosis infection and thereby improving preventive health behaviors for avoiding leptospirosis.
Digital Training for Non-Specialist Health Workers to Deliver a Brief Psychological Treatment for Depression in Primary Care in India: Findings from a Randomized Pilot Study
Authors: Muke Shital S. , Tugnawat Deepak , Joshi Udita , Anand Aditya , Khan Azaz , Shrivastava Ritu , Singh Abhishek , Restivo Juliana L. , Bhan Anant , Patel Vikram , Naslund John A.
Published: September 2020

Introduction: Task sharing holds promise for scaling up depression care in countries such as India, yet requires training large numbers of non-specialist health workers. This pilot trial evaluated the feasibility and acceptability of a digital program for training non-specialist health workers to deliver a brief psychological treatment for depression. Methods: Participants were non-specialist health workers recruited from primary care facilities in Sehore, a rural district in Madhya Pradesh, India. A three-arm randomized controlled trial design was used, comparing digital training alone (DGT) to digital training with remote support (DGT+), and conventional face-to-face training. The primary outcome was the feasibility and acceptability of digital training programs. Preliminary effectiveness was explored as changes in competency outcomes, assessed using a self-reported measure covering the specific knowledge and skills required to deliver the brief psychological treatment for depression. Outcomes were collected at pre-training and post-training. Results: Of 42 non-specialist health workers randomized to the training programs, 36 including 10 (72%) in face-to-face, 12 (86%) in DGT, and 14 (100%) in DGT+ arms started the training. Among these participants, 27 (64%) completed the training, with 8 (57%) in face-to-face, 8 (57%) in DGT, and 11 (79%) in DGT+. The addition of remote telephone support appeared to improve completion rates for DGT+ participants. The competency outcome improved across all groups, with no significant between-group differences. However, face-to-face and DGT+ participants showed greater improvement compared to DGT alone. There were numerous technical challenges with the digital training program such as poor connectivity, smartphone app not loading, and difficulty navigating the course content—issues that were further emphasized in follow-up focus group discussions with participants. Feedback and recommendations collected from participants informed further modifications and refinements to the training programs in preparation for a forthcoming large-scale effectiveness trial. Conclusions: This study adds to mounting efforts aimed at leveraging digital technology to increase the availability of evidence-based mental health services in primary care settings in low-resource settings.
Factors and Beliefs that Condition the Attitude of Health Science Students towards End of Life in Spain and Bolivia: A Multicenter Study
Authors: Pérez-de la Cruz Sagrario , Ramírez Ivonne , Maldonado César
Published: September 2020

Health Science students in Spain and Bolivia should be trained in the management of the processes of death and dying of patients. The aim of this study was to examine the degree of training, self-perceived safety and preferences in relation to the care of terminal and non-terminal patients. It was a descriptive, cross-sectional, multicenter study with students of Medicine, Nursing and Physiotherapy in Spain and Bolivia. The following variables were evaluated: care preparation and emotional preparation to caring for terminally ill and non-terminally ill patients, the Death Attitude Profile Revised (PAM-R) and the Bugen Scale for Facing Death. The self-perceived preparation of students for caring for terminally ill patients can be considered “fair” (mean 2.15, SD 0.756), and this was also the case for their perceived emotional preparation (mean 2.19, SD 0.827). In contrast, the score obtained for their preparedness for treating non-terminal patients was higher (mean 2.99 and 3.16, respectively). Working with terminally ill patients, including terminal or geriatric cancer patients, was the least preferred option among future health professionals. The results obtained show a limited preference for end-of-life care and treatment, highlighting a lack of preparation and motivation among health science students in Spain and Bolivia for working with these patients.
Aryl Hydrocarbon Receptor Signaling Is Functional in Immune Cells of Rainbow Trout (Oncorhynchus mykiss)
Authors: Song Jun-Young , Casanova-Nakayama Ayako , Möller Anja-Maria , Kitamura Shin-Ichi , Nakayama Kei , Segner Helmut
Published: September 2020

The arylhydrocarbon receptor (AhR) is an important signaling pathway in the immune system of mammals. In addition to its physiological functions, the receptor mediates the immunotoxic actions of a diverse range of environmental contaminants that bind to and activate the AhR, including planar halogenated aromatic hydrocarbons (PHAHs or dioxin-like compounds) and polynuclear aromatic hydrocarbons (PAHs). AhR-binding xenobiotics are immunotoxic not only to mammals but to teleost fish as well. To date, however, it is unknown if the AhR pathway is active in the immune system of fish and thus may act as molecular initiating event in the immunotoxicity of AhR-binding xenobiotics to fish. The present study aims to examine the presence of functional AhR signaling in immune cells of rainbow trout (Oncorhynchus mykiss). Focus is given to the toxicologically relevant AhR2 clade. By means of RT-qPCR and in situ hybdridization, we show that immune cells of rainbow trout express ahr 2α and ahr 2β mRNA; this applies for immune cells isolated from the head kidney and from the peripheral blood. Furthermore, we show that in vivo as well as in vitro exposure to the AhR ligand, benzo(a)pyrene (BaP), causes upregulation of the AhR-regulated gene, cytochrome p4501a, in rainbow trout immune cells, and that this induction is inhibited by co-treatment with an AhR antagonist. Taken together, these findings provide evidence that functional AhR signaling exists in the immune cells of the teleost species, rainbow trout.
The Effect of Dysfunctional Ubiquitin Enzymes in the Pathogenesis of Most Common Diseases
Authors: Celebi Gizem , Kesim Hale , Ozer Ebru , Kutlu Ozlem
Published: September 2020

Ubiquitination is a multi-step enzymatic process that involves the marking of a substrate protein by bonding a ubiquitin and protein for proteolytic degradation mainly via the ubiquitin–proteasome system (UPS). The process is regulated by three main types of enzymes, namely ubiquitin-activating enzymes (E1), ubiquitin-conjugating enzymes (E2), and ubiquitin ligases (E3). Under physiological conditions, ubiquitination is highly reversible reaction, and deubiquitinases or deubiquitinating enzymes (DUBs) can reverse the effect of E3 ligases by the removal of ubiquitin from substrate proteins, thus maintaining the protein quality control and homeostasis in the cell. The dysfunction or dysregulation of these multi-step reactions is closely related to pathogenic conditions; therefore, understanding the role of ubiquitination in diseases is highly valuable for therapeutic approaches. In this review, we first provide an overview of the molecular mechanism of ubiquitination and UPS; then, we attempt to summarize the most common diseases affecting the dysfunction or dysregulation of these mechanisms.
Using a Simple Cellular Assay to Map NES Motifs in Cancer-Related Proteins, Gain Insight into CRM1-Mediated NES Export, and Search for NES-Harboring Micropeptides
Authors: Sendino Maria , Omaetxebarria Miren Josu , Prieto Gorka , Rodriguez Jose Antonio
Published: September 2020

The nuclear export receptor CRM1 (XPO1) recognizes and binds specific sequence motifs termed nuclear export signals (NESs) in cargo proteins. About 200 NES motifs have been identified, but over a thousand human proteins are potential CRM1 cargos, and most of their NESs remain to be identified. On the other hand, the interaction of NES peptides with the “NES-binding groove” of CRM1 was studied in detail using structural and biochemical analyses, but a better understanding of CRM1 function requires further investigation of how the results from these in vitro studies translate into actual NES export in a cellular context. Here we show that a simple cellular assay, based on a recently described reporter (SRVB/A), can be applied to identify novel potential NESs motifs, and to obtain relevant information on different aspects of CRM1-mediated NES export. Using cellular assays, we first map 19 new sequence motifs with nuclear export activity in 14 cancer-related proteins that are potential CRM1 cargos. Next, we investigate the effect of mutations in individual NES-binding groove residues, providing further insight into CRM1-mediated NES export. Finally, we extend the search for CRM1-dependent NESs to a recently uncovered, but potentially vast, set of small proteins called micropeptides. By doing so, we report the first NES-harboring human micropeptides.
Cellular Signaling and Anti-Apoptotic Effects of Prolactin-Releasing Peptide and Its Analog on SH-SY5Y Cells
Authors: Zmeškalová Anna , Popelová Andrea , Exnerová Aneta , Železná Blanka , Kuneš Jaroslav , Maletínská Lenka
Published: September 2020

Prolactin-releasing peptide (PrRP), a natural ligand for the GPR10 receptor, is a neuropeptide with anorexigenic and antidiabetic properties. Due to its role in the regulation of food intake, PrRP is a potential drug for obesity treatment and associated type 2 diabetes mellitus (T2DM). Recently, the neuroprotective effects of lipidized PrRP analogs have been proven. In this study, we focused on the molecular mechanisms of action of natural PrRP31 and its lipidized analog palm11-PrRP31 in the human neuroblastoma cell line SH-SY5Y to describe their cellular signaling and possible anti-apoptotic properties. PrRP31 significantly upregulated the phosphoinositide-3 kinase-protein kinase B/Akt (PI3K-PKB/Akt) and extracellular signal-regulated kinase/cAMP response element-binding protein (ERK-CREB) signaling pathways that promote metabolic cell survival and growth. In addition, we proved via protein kinase inhibitors that activation of signaling pathways is mediated specifically by PrRP31 and its palmitoylated analog. Furthermore, the potential neuroprotective properties were studied through activation of anti-apoptotic pathways of PrRP31 and palm11-PrRP31 using the SH-SY5Y cell line and rat primary neuronal culture stressed with toxic methylglyoxal (MG). The results indicate increased viability of the cells treated with PrRP and palm11-PrRP31 and a reduced degree of apoptosis induced by MG, suggesting their potential use in the treatment of neurological disorders.
Selection and Characterization of YKL-40-Targeting Monoclonal Antibodies from Human Synthetic Fab Phage Display Libraries
Authors: Kang Kyungjae , Kim Kicheon , Lee Se-Ra , Kim Yoonji , Lee Joo Eon , Lee Yong Sun , Lim Ju-Hyeon , Lim Chung-Su , Kim Yu Jung , Baek Seung Il , Song Du Hyun , Hong Jin Tae , Kim Dae Young
Published: September 2020

YKL-40, also known as chitinase-3-like 1 (CHI3L1), is a glycoprotein that is expressed and secreted by various cell types, including cancers and macrophages. Due to its implications for and upregulation in a variety of diseases, including inflammatory conditions, fibrotic disorders, and tumor growth, YKL-40 has been considered as a significant therapeutic biomarker. Here, we used a phage display to develop novel monoclonal antibodies (mAbs) targeting human YKL-40 (hYKL-40). Human synthetic antibody phage display libraries were panned against a recombinant hYKL-40 protein, yielding seven unique Fabs (Antigen-binding fragment), of which two Fabs (H1 and H2) were non-aggregating and thermally stable (75.5 °C and 76.5 °C, respectively) and had high apparent affinities (KD = 2.3 nM and 4.0 nM, respectively). Reformatting the Fabs into IgGs (Immunoglobulin Gs) increased their apparent affinities (notably, for H1 and H2, KD = 0.5 nM and 0.3 nM, respectively), presumably due to the effects of avidity, with little change to their non-aggregation property. The six anti-hYKL-40 IgGs were analyzed using a trans-well migration assay in vitro, revealing that three clones (H1, H2, and H4) were notably effective in reducing cell migration from both A549 and H460 lung cancer cell lines. The three clones were further analyzed in an in vivo animal test that assessed their anti-cancer activities, demonstrating that the tumor area and the number of tumor nodules were significantly reduced in the lung tissues treated with H1 (IgG). Given its high affinity and desirable properties, we expect that the H1 anti-hYKL-40 mAb will be a suitable candidate for developing anti-cancer therapeutics.
Parasitoid Abundance and Community Composition in Desert Vineyards and Their Adjacent Natural Habitats
Authors: Segoli Michal , Kishinevsky Miriam , Rozenberg Tamir , Hoffmann Ishai
Published: September 2020

Parasitoids are important natural enemies of many agricultural pests. Preserving natural habitats around agricultural fields may support parasitoid populations. However, the success of such an approach depends on the ability of parasitoids to utilize both crop and natural habitats. While these aspects have been studied extensively in temperate regions, very little is known about parasitoid communities in desert agroecosystems. We took one step in this direction by sampling parasitoids in six vineyards and their surrounding natural desert habitat in a hyper-arid region of the Negev Desert Highlands, Israel. We predicted that due to the high contrast in environmental conditions, parasitoid abundance and community composition would differ greatly between the crop and the natural desert habitats. We found that parasitoid abundance differed between the habitats; however, the exact distribution pattern depended on the time of year—with higher numbers of parasitoids in the natural habitat at the beginning of the vine growth season and higher numbers in the vineyard at the middle and end of the season. Although parasitoid community composition significantly differed between the vineyard and desert habitats, this only accounted for ~4% of the total variation. Overall, our results do not strongly support the notion of distinct parasitoid communities in the crop vs. the desert environment, suggesting that despite environmental contrasts, parasitoids may move between and utilize resources in both habitats.