bluuebunny/crossref_metadata_2025 huggingface dataset:
Comparative Otolith Morphology of Two Morphs of Schizopygopsis thermalis Herzenstein 1891 (Pisces, Cyprinidae) in a Headwater Lake on the Qinghai-Tibet Plateau
Authors: Qiao Jialing , Zhu Ren , Chen Kang , Zhang Dong , Yan Yunzhi , He Dekui
Published: April 2022

Teleost otoliths provide a pivotal medium for studying changes in population structure and population dynamics of fish. Understanding the otolith-fish size relationship and intraspecies variation in otolith morphology is essential for the accurate assessment and management of fishery resources. In our study, we aimed to estimate the relationships between otolith morphological measurements and fish length, and detect differences in the otolith morphology of planktivorous and benthivorous morphs of Schizopygopsis thermalis in Lake Amdo Tsonak Co on the Qinghai-Tibet Plateau (QTP). Both morphs exhibited strong linear otolith-fish size relationships; otolith morphology was sexually dimorphic in each morph; the morphs differed significantly in otolith shape and size (e.g., posterior side, the region between the posterior and ventral otolith, otolith length, circularity, and surface density). In addition, we found that the differences in otolith morphology between morphs are related to habitat preferences, diet, and growth. Basic data on the biology of S. thermalis are essential for poorly studied Lake Amdo Tsonak Co, and our study emphasizes that intraspecific variation in otolith morphology should be taken into consideration when differentiating stocks, populations, and age classes based on otolith morphology.
Numerical and Experimental Investigation of the Effect of Micro Restriction Geometry on Gas Flows through a Micro Orifice
Authors: Aboubakri Akam , Sadaghiani Abdolali Khalili , Akgonul Sarp , Erdoğmuş Abdullah Berkan , Baki Murat , Can Fatih , Sabanovic Asif , Koşar Ali
Published: April 2022

This study experimentally and numerically investigates the effects of the nozzle/needle distance (clearance) and supply pressure on single phase compressible gas flow in a micro orifice with needle restriction, which play important roles in many engineering applications such as cryogenic cooling and MEMS (microelectromechanical systems) device cooling. Nitrogen was used as the working fluid at supply pressures ranging from 10 to 50 bars, while the conical needle draft angle was 15°. The nozzle/needle distance (clearance) was changed from 100 µm to 500 µm. From the experimental point of view, the load provided by the working fluid over the needle was measured by a load sensor. For the numerical analysis, six turbulence models and three wall treatments were considered in numerical simulations. The effect of micro restriction on high-pressure micro-gas flows was further assessed by numerical modeling. It is evident from the results that the utilized turbulence model has a considerable effect on the computed results. The k–ε standard and Spalart–Allmaras models were found to be not suitable for modeling micro-scale gas flows with restriction. On the other hand, the k–ε realizable and k–ω SST models exhibit the best performance in predicting the results.
Functionalities of Gelatin Modified with 2-Octenyl Succinic Anhydride and Gallic Acid
Authors: Liu Tai-Ti , Zhuang Xin-Yi , Yang Tsung-Shi
Published: April 2022

The aim of this research was to modify gelatin (GT) with 2-octenyl succinic anhydride (OA) and gallic acid (GA) and investigate its functionalities. GT modified with OA (GT-OA) has an improved water solubility at room temperature and an enhanced surface activity, foaming capacity, and pH buffering ability. Regarding antioxidant activity, GT-OA grafted with GA to generate the compound GT-OA-GA has shown good antioxidant activity. Particularly, GT-OA-GA surpassed GA in ferrous ion (Fe2+)-chelating activity. With respect to antimicrobial activity, GT-OA-GA could be complexed with zinc ions (Zn2+), and this complex exhibited good antimicrobial activity against Staphylococcus aureus and Escherichia coli (O157:H7). Chemically modified GT has better water solubility at room temperature and more functionalities than unmodified GT. Thus, it can be used as an emulsifier or coating material in food, cosmetic, and pharmaceutical industries pertaining to GT applications.
On Some Variational Inequalities Involving Second-Order Partial Derivatives
Authors: Treanţă Savin , Khan Muhammad Bilal , Saeed Tareq
Published: April 2022

By using the monotonicity, hemicontinuity, and pseudomonotonicity of the considered integral functionals, we studied the well-posedness of some variational inequality problems governed by partial derivatives of the second-order. To this aim, we introduce the approximating solution set and the concept of approximating sequences for the considered controlled variational inequality problem. Further, by using the aforementioned new mathematical tools, we established some theorems on well-posedness. Moreover, the theoretical tools and results included in the paper are accompanied by some examples.
Hyaluronidase 6 Does Not Affect Cumulus–Oocyte Complex Dispersal and Male Mice Fertility
Authors: Bang Hyewon , Lee Sujin , Jeong Pil-Soo , Seol Dong-Won , Son Daeun , Kim Young-Hyun , Song Bong-Seok , Sim Bo-Woong , Park Soojin , Lee Dong-Mok , Wee Gabbine , Park Joon-Suk , Kim Sun-Uk , Kim Ekyune
Published: April 2022

Glycosylphosphatidylinositol-anchored sperm hyaluronidases (HYAL) assist sperm penetration through the cumulus–oocyte complex (COC), but their role in mammalian fertilization remains unclear. Previously, we demonstrated that sperm from HYAL 5 and 7 double-knockout (dKO) mice produced significantly less offspring than sperm from wild-type mice due to defective COC dispersal. However, the HYAL6 gene remained active in the sperm from the dKO mice, indicating that they were not entirely infertile. This study explored the role of HYAL6 in fertilization by analyzing HYAL6-mutant mice. In this mouse model, HYAL5 and HYAL7 were present in the HYAL6-knockout sperm, and they could disperse hyaluronic acid. We found that HYAL6 was present on the surface of sperm. However, male mice lacking the HYAL6 gene had normal fertility, testicular integrity, and sperm characteristics. Furthermore, in vitro fertilization assays demonstrated that HYAL6-deficient epididymal sperm functioned normally. Therefore, HYAL6 is dispensable for fertilization.
SCN1A Variants as the Underlying Cause of Genetic Epilepsy with Febrile Seizures Plus in Two Multi-Generational Colombian Families
Authors: Cornejo-Sanchez Diana M. , Acharya Anushree , Bharadwaj Thashi , Marin-Gomez Lizeth , Pereira-Gomez Pilar , Nouel-Saied Liz M. ,   University of Washington Center for Mendelian Genomics, Nickerson Deborah A. , Bamshad Michael J. , Mefford Heather C. , Schrauwen Isabelle , Carrizosa-Moog Jaime , Cornejo-Ochoa William , Pineda-Trujillo Nicolas , Leal Suzanne M.
Published: April 2022

Genetic epilepsy with febrile seizures plus (GEFS+) is an autosomal dominant disorder with febrile or afebrile seizures that exhibits phenotypic variability. Only a few variants in SCN1A have been previously characterized for GEFS+, in Latin American populations where studies on the genetic and phenotypic spectrum of GEFS+ are scarce. We evaluated members in two multi-generational Colombian Paisa families whose affected members present with classic GEFS+. Exome and Sanger sequencing were used to detect the causal variants in these families. In each of these families, we identified variants in SCN1A causing GEFS+ with incomplete penetrance. In Family 047, we identified a heterozygous variant (c.3530C > G; p.(Pro1177Arg)) that segregates with GEFS+ in 15 affected individuals. In Family 167, we identified a previously unreported variant (c.725A > G; p.(Gln242Arg)) that segregates with the disease in a family with four affected members. Both variants are located in a cytoplasmic loop region in SCN1A and based on our findings the variants are classified as pathogenic and likely pathogenic, respectively. Our results expand the genotypic and phenotypic spectrum associated with SCN1A variants and will aid in improving molecular diagnostics and counseling in Latin American and other populations.
Genetic Variability of Inflammation and Oxidative Stress Genes Affects Onset, Progression of the Disease and Survival of Patients with Amyotrophic Lateral Sclerosis
Authors: Ravnik-Glavač Metka , Goričar Katja , Vogrinc David , Koritnik Blaž , Lavrenčič Jakob Gašper , Glavač Damjan , Dolžan Vita
Published: April 2022

Inflammation and oxidative stress are recognized as important contributors to amyotrophic lateral sclerosis (ALS) disease pathogenesis. Our aim was to evaluate the impact of selected single-nucleotide polymorphisms in genes involved in inflammation and oxidative stress on ALS susceptibility and modification. One-hundred-and-eighty-five ALS patients and 324 healthy controls were genotyped for nine polymorphisms in seven antioxidant and inflammatory genes using competitive allele-specific PCR. Logistic regression; nonparametric tests and survival analysis were used in the statistical analysis. Investigated polymorphisms were not associated with ALS susceptibility. Carriers of at least one polymorphic SOD2 rs4880 T or IL1B rs1071676 C allele more often had bulbar ALS onset (p = 0.036 and p = 0.039; respectively). IL1B rs1071676 was also associated with a higher rate of disease progression (p = 0.015). After adjustment for clinical parameters; carriers of two polymorphic IL1B rs1071676 C alleles had shorter survival (HR = 5.02; 95% CI = 1.92–13.16; p = 0.001); while carriers of at least one polymorphic CAT rs1001179 T allele had longer survival (HR = 0.68; 95% CI = 0.47–0.99; p = 0.046). Our data suggest that common genetic variants in the antioxidant and inflammatory pathways may modify ALS disease. Such genetic information could support the identification of patients that may be responsive to the immune or antioxidant system—based therapies.
Reduction of BMPR2 mRNA Expression in Peripheral Blood of Pulmonary Arterial Hypertension Patients: A Marker for Disease Severity?
Authors: Theobald Vivienne , Benjamin Nicola , Seyfarth Hans-Jürgen , Halank Michael , Schneider Marc A. , Richtmann Sarah , Hinderhofer Katrin , Xanthouli Panagiota , Egenlauf Benjamin , Seeger Rebekka , Hoeper Marius M. , Jonigk Danny , Grünig Ekkehard , Eichstaedt Christina A.
Published: April 2022

Pulmonary arterial hypertension (PAH) can be caused by pathogenic variants in the gene bone morphogenetic protein receptor 2 (BMPR2). While BMPR2 protein expression levels are known to be reduced in the lung tissue of heritable PAH (HPAH) patients, a systematic study evaluating expression in more easily accessible blood samples and its clinical relevance is lacking. Thus, we analyzed the BMPR2 mRNA expression in idiopathic/HPAH patients and healthy controls in blood by quantitative polymerase chain reaction and protein expression by enzyme-linked immunosorbent assay. Clinical parameters included right heart catherization, echocardiography, six-minute walking test and laboratory tests. BMPR2 variant-carriers (n = 23) showed significantly lower BMPR2 mRNA expression in comparison to non-carriers (n = 56) and healthy controls (n = 30; p < 0.0001). No difference in BMPR2 protein expression was detected. Lower BMPR2 mRNA expression correlated significantly with greater systolic pulmonary artery pressure and pulmonary vascular resistance. Higher BMPR2 mRNA expression correlated with greater glomerular filtration rate, cardiac index and six-minute walking distance. We demonstrated the feasibility to assess BMPR2 expression in blood and, for the first time, that BMPR2 mRNA expression levels are significantly reduced in variant carriers and correlated with clinical parameters. Further studies may evaluate the usefulness of BMPR2 mRNA expression in blood as a new marker for disease severity.
p53N236S Activates Autophagy in Response to Hypoxic Stress Induced by DFO
Authors: Gao Kang , Zong Huanhuan , Hou Kailong , Zhang Yanduo , Zhang Ruyi , Zhao Dan , Guo Xin , Luo Ying , Jia Shuting
Published: April 2022

Hypoxia can lead to stabilization of the tumor suppressor gene p53 and cell death. However, p53 mutations could promote cell survival in a hypoxic environment. In this study, we found that p53N236S (p53N239S in humans, hereinafter referred to as p53S) mutant mouse embryonic fibroblasts (MEFs) resistant to deferoxamine (DFO) mimic a hypoxic environment. Further, Western blot and flow cytometry showed reduced apoptosis in p53S/S cells compared to WT after DFO treatment, suggesting an antiapoptosis function of p53S mutation in response to hypoxia-mimetic DFO. Instead, p53S/S cells underwent autophagy in response to hypoxia stress presumably through inhibition of the AKT/mTOR pathway, and this process was coupled with nuclear translocation of p53S protein. To understand the relationship between autophagy and apoptosis in p53S/S cells in response to hypoxia, the autophagic inhibitor 3-MA was used to treat both WT and p53S/S cells after DFO exposure. Both apoptotic signaling and cell death were enhanced by autophagy inhibition in p53S/S cells. In addition, the mitochondrial membrane potential (MMP) and the ROS level results indicated that p53S might initiate mitophagy to clear up damaged mitochondria in response to hypoxic stress, thus increasing the proportion of intact mitochondria and maintaining cell survival. In conclusion, the p53S mutant activates autophagy instead of inducing an apoptotic process in response to hypoxia stress to protect cells from death.
Network Approaches for Charting the Transcriptomic and Epigenetic Landscape of the Developmental Origins of Health and Disease
Authors: Lombardo Salvo Danilo , Wangsaputra Ivan Fernando , Menche Jörg , Stevens Adam
Published: April 2022

The early developmental phase is of critical importance for human health and disease later in life. To decipher the molecular mechanisms at play, current biomedical research is increasingly relying on large quantities of diverse omics data. The integration and interpretation of the different datasets pose a critical challenge towards the holistic understanding of the complex biological processes that are involved in early development. In this review, we outline the major transcriptomic and epigenetic processes and the respective datasets that are most relevant for studying the periconceptional period. We cover both basic data processing and analysis steps, as well as more advanced data integration methods. A particular focus is given to network-based methods. Finally, we review the medical applications of such integrative analyses.
Contradictory Phylogenetic Signals in the Laurasiatheria Anomaly Zone
Authors: Doronina Liliya , Hughes Graham M. , Moreno-Santillan Diana , Lawless Colleen , Lonergan Tadhg , Ryan Louise , Jebb David , Kirilenko Bogdan M. , Korstian Jennifer M. , Dávalos Liliana M. , Vernes Sonja C. , Myers Eugene W. , Teeling Emma C. , Hiller Michael , Jermiin Lars S. , Schmitz Jürgen , Springer Mark S. , Ray David A.
Published: April 2022

Relationships among laurasiatherian clades represent one of the most highly disputed topics in mammalian phylogeny. In this study, we attempt to disentangle laurasiatherian interordinal relationships using two independent genome-level approaches: (1) quantifying retrotransposon presence/absence patterns, and (2) comparisons of exon datasets at the levels of nucleotides and amino acids. The two approaches revealed contradictory phylogenetic signals, possibly due to a high level of ancestral incomplete lineage sorting. The positions of Eulipotyphla and Chiroptera as the first and second earliest divergences were consistent across the approaches. However, the phylogenetic relationships of Perissodactyla, Cetartiodactyla, and Ferae, were contradictory. While retrotransposon insertion analyses suggest a clade with Cetartiodactyla and Ferae, the exon dataset favoured Cetartiodactyla and Perissodactyla. Future analyses of hitherto unsampled laurasiatherian lineages and synergistic analyses of retrotransposon insertions, exon and conserved intron/intergenic sequences might unravel the conflicting patterns of relationships in this major mammalian clade.
Five Fabaceae Karyotype and Phylogenetic Relationship Analysis Based on Oligo-FISH for 5S rDNA and (AG3T3)3
Authors: He Zhoujian , Zhang Wei , Luo Xiaomei , Huan Jie
Published: April 2022

Most Fabaceae have nitrogen fixation abilities and are valuable forage and medicinal resources. However, cytogenetic data of many Fabaceae species are unclear. Karyotypes reveal cytological characteristics and are crucial to understanding the organization and evolution of chromosomes in species. Oligo-FISH can reveal genetic composition and karyotype variation patterns with rapid and efficient results. Karyotype analysis of five Fabaceae species by oligonucleotide probes showed that: Robinia pseudoacacia, karyotype formula 2n = 2x = 20m + 2sm, cytotype 2B, arm ratio 3.4821, eight chromosomes distributed 5S rDNA signal. The karyotype formula of Robinia pseudoacacia ‘idaho’ was 2n = 2x = 20m + 2sm, cytotype 1A, arm ratio 1.8997, and 5S rDNA signal was distributed on six chromosomes. Karyotype of Robinia pseudoacacia f. decaisneana 2n = 2x = 20m + 2sm, cytotype 1B, arm ratio 2.0787, the distribution of eight chromosomes with 5S rDNA signal. Karyotype formula of Styphnolobium japonicum 2n = 2x = 14m + 12sm + 2st, cytotype 2B, arm ratio 2.6847, two chromosomes have 5S rDNA signal. Amorpha fruticose karyotype 2n = 2x = 38m + 2sm, cytotype 1B, arm ratio 3.2058, four chromosomes possessed 5S rDNA signal. Both ends of all species’ chromosomes have (AG3T3)3 signals. The results of this study provide chromosome numbers and a physical map, contributing to the construction of the Oligo-FISH barcode and providing molecular cytogenetics data for Fabaceae.
Using Molecular Tools to Understand Microbial Carbonates
Authors: Cutts Elise M. , Baldes Matthew J. , Skoog Emilie J. , Hall James , Gong Jian , Moore Kelsey R. , Bosak Tanja
Published: April 2022

Here we review the application of molecular biological approaches to mineral precipitation in modern marine microbialites. The review focuses on the nearly two decades of nucleotide sequencing studies of the microbialites of Shark Bay, Australia; and The Bahamas. Molecular methods have successfully characterized the overall community composition of mats, pinpointed microbes involved in key metabolisms, and revealed patterns in the distributions of microbial groups and functional genes. Molecular tools have become widely accessible, and we can now aim to establish firmer links between microbes and mineralization. Two promising future directions include “zooming in” to assess the roles of specific organisms, microbial groups, and surfaces in carbonate biomineralization and “zooming out” to consider broader spans of space and time. A middle ground between the two can include model systems that contain representatives of important microbial groups, processes, and metabolisms in mats and simplify hypothesis testing. These directions will benefit from expanding reference datasets of marine microbes and enzymes and enrichments of representative microbes from mats. Such applications of molecular tools should improve our ability to interpret ancient and modern microbialites and increase the utility of these rocks as long-term recorders of microbial processes and environmental chemistry.
Application of Chemostratigraphic Methods to Floodplain Alluvial Deposits within the Big Harris Creek Basin, North Carolina
Authors: Sullivan Samantha N. , Miller Jerry R. , Huffman Carmen L.
Published: April 2022

Big Harris Creek, North Carolina, possesses a geomorphic history and alluvial stratigraphic record similar to many drainages in southern Appalachian Piedmont. An approximately 1 km reach of Upper Stick Elliott Creek, a tributary to Big Harris Creek, was used herein to (1) explore the use of chemostratigraphic methods to define and correlate late Holocene alluvial deposits along this relatively uncontaminated rural stream containing legacy sediments (historic, anthropogenically derived deposits), and (2) interpret depositional floodplain processes within small (<10 km2), headwater drainages. The lithofacies within four floodplain sections were described in channel banks and sampled at about 5 cm intervals. The 128 collected samples were then analyzed for grain size and the concentration of 22 elements using X-ray fluorescence. Well-defined chemostratigraphic units (facies) were defined on the basis of a multi-elemental fingerprint using a principal component analysis (PCA) and verified using discriminant analysis (DA). Chemostratigraphic units did not reflect grain size at a site (by design) but marginally correlated to lithofacies defined by field descriptions. Of significant importance, chemostratigraphic units could be quantitatively correlated between the four stratigraphic sections at a much higher spatial resolution (~5 cm) than could be performed using other sedimentologic parameters alone. In combination, the lithostratigraphic and chemostratigraphic architecture of the floodplain is consistent with a previously proposed sequence of deposition for the legacy deposits in which extensive land-use change associated with the onset of cotton farming in the 1860s led to upstream incision and gully formation and downstream deposition on the floodplain surface. Deposition appears to have progressed downvalley as incision deepened, probably in the form of crevasse splay deposits or proximal sandsheets that were occasionally interbedded with vertically accreted sediments. The results indicate that chemostratigraphy represents a highly useful approach to the assessment of floodplain depositional processes over (at least) relatively small temporal and spatial scales, even in areas with minimal sediment contamination.
Mitigation of Salinity Stress on Pomegranate (Punica granatum L. cv. Wonderful) Plant Using Salicylic Acid Foliar Spray
Authors: Khalil Hoda A. , El-Ansary Diaa O. , Ahmed Zienab F. R.
Published: April 2022

Salt stress significantly impacts plant morphological structure and physiological processes, resulting in decreased plant growth. Salicylic acid (SA) is a key signal molecule that protects plants from the negative impacts of salinity. Under natural conditions, the pomegranate plant generally exhibits salt-tolerant characteristics. The objective of this study was to elucidate the salt-tolerance level of pomegranate (Punica granatum L. cv. Wonderful) and the effect of the regulating strategy of SA foliar spray on growth, morphological structure, and physiological processes. SA levels were 0, 0.25, 0.50, and 1 mM in the presence of salinity levels of 10, 35, and 70 mM NaCl, respectively. Vegetative growth indices, including stem cross-sectional area, leaf area, and total dry weight, were lowered by salinity treatments. However, SA applications greatly improved morphological characteristics and plant growth under salt stress. The effects of salinity were effectively reversed by SA treatment at 1 mM compared to control and other treatments. Interestingly, SA applications enhanced the chlorophyll, total phenolic, carbohydrate, and proline contents of leaves while decreasing electrolyte leakage (EL), Na, and Cl levels. Moreover, the foliar SA treatments enhanced the nutrient content in the leaves and increased the activities of peroxidase (POD) and catalase (CAT), with a decrease in malondialdehyde (MDA) content. This study suggests that the alleviation of the salinity stress by SA may be due to the activation of the antioxidant enzymatic mechanism and decrease in the lipid peroxidation of the pomegranate plant.
Caregivers’ Difficulty in Managing Smartphone Use of Children with Attention-Deficit/Hyperactivity Disorder during the COVID-19 Pandemic: Relationships with Caregiver and Children Factors
Authors: Lee Jia-In , Hsiao Ray C. , Tsai Ching-Shu , Yen Cheng-Fang
Published: April 2022

This study examined the difficulty encountered by caregivers of children with attention-deficit/hyperactivity disorder (ADHD) in managing children’s smartphone use during the coronavirus disease 2019 (COVID-19) pandemic and the caregiver- and children-related factors that influence this difficulty. In total, 252 caregivers of children with ADHD were recruited into this study. The caregivers completed a research questionnaire to provide data regarding the difficulty they encountered in managing the smartphone use of children during the COVID-19 pandemic, their general mental health and parenting styles, and the ADHD and oppositional defiant disorder (ODD) symptoms of the children they are caring for. The results indicated that almost 45% of the caregivers of children with ADHD sometimes or often found it difficult to manage the smartphone use of children with ADHD during the COVID-19 pandemic. For the caregivers, a short duration of education, poor general mental health, unaffectionate/uncaring and overprotective parenting styles, older children, and inattention and ODD symptoms were significantly associated with increased difficulty in managing their children’s smartphone use during the COVID-19 pandemic. On the basis of the relevant factors identified in this study, an intervention should be developed to enhance the skills of caregivers of children with ADHD with respect to the management of children’s smartphone use during the COVID-19 pandemic.
Whole Body Vibration Exposure Transmitted to Drivers of Heavy Equipment Vehicles: A Comparative Case According to the Short- and Long-Term Exposure Assessment Methodologies Defined in ISO 2631-1 and ISO 2631-5
Authors: de la Hoz-Torres María L. , Aguilar Antonio J. , Ruiz Diego P. , Martínez-Aires Mª Dolores
Published: April 2022

The construction and transport sectors are the industries with the highest proportions of workers exposed to vibrations in the European Union. Heavy equipment vehicle (HEV) drivers often perform operations on different uneven surfaces and are exposed to whole body vibration (WBV) on a daily basis. Recently, a new version of ISO 2631-5 was published. However, since this new method required as input the individual exposure profile and the acceleration signals recorded on more surfaces, limited studies have been carried out to evaluate HEV operations according to this standard. The objectives of this study were to assess the WBV exposure using the methods defined in ISO 2631-1:1997 and ISO 2631-5:2018 and to compare the obtained health risk assessments between drivers with different anthropometric characteristics. For this purpose, two drivers were selected and a field measurement campaign was conducted. Regarding short-term assessment, results showed that VDV was the most restrictive method with exposure levels above the exposure action limit value, while SdA indicated that the same exposures were safe for the worker. With respect to long-term assessment, Risk Factor RA showed that the driver with the highest body mass index was the only one who exceeded the low probability limit of adverse health effects.
Motivational Factors Are Varying across Age Groups and Gender
Authors: Sigmundsson Hermundur , Haga Monika , Elnes Magdalena , Dybendal Benjamin Holen , Hermundsdottir Fanny
Published: April 2022

The aim of the current study was to explore differences in passion for achievement, grit, and mindset across age and gender, by using a cross-sectional design. The sample consisted of 1548 participants including 931 females and 617 males aged from 13 to 77 years (Mage 26.53 years, SD = 11.77). The eight-item Passion for Achievement Scale was used to assess general passion and the Grit-S scale was used to assess grit. Mindset was assessed using the eight-item Theories of Intelligence Scale (TIS). The results indicated significant differences between the three factors related to age, age groups, and gender. For the total sample, there was a significant gender difference in passion, where males score higher, and growth mindset, where females score higher. With age, passion decreases until the age of 50–59, and slightly increases for the remaining age groups. After a decrease in grit between the first (13–19 years) and the second (20–29 years) age group, grit increases with age. Mindset scores decline strongly after the age of 40–49. Generally, the patterns show that mindset and passion decrease across the life-span, while grit increases. Indeed, these attributes seems to be different from each other, and how they change varies across age groups.
Evaluation Methods Applied to Digital Health Interventions: What Is Being Used beyond Randomised Controlled Trials?—A Scoping Review
Authors: Hrynyschyn Robert , Prediger Christina , Stock Christiane , Helmer Stefanie Maria
Published: April 2022

Despite the potential of digital health interventions (DHIs), evaluations of their effectiveness face challenges. DHIs are complex interventions and currently established evaluation methods, e.g., the randomised controlled trial (RCT), are limited in their application. This study aimed at identifying alternatives to RCTs as potentially more appropriate evaluation approaches. A scoping review was conducted to provide an overview of existing evaluation methods of DHIs beyond the RCT. Cochrane Central Register of Controlled Trials, MEDLINE, Web of Science, and EMBASE were screened in May 2021 to identify relevant publications, while using defined inclusion and exclusion criteria. Eight studies were extracted for a synthesis comprising four alternative evaluation designs. Factorial designs were mostly used to evaluate DHIs followed by stepped-wedge designs, sequential multiple assignment randomised trials (SMARTs), and micro randomised trials (MRTs). Some of these methods allow for the adaptation of interventions (e.g., SMART or MRT) and the evaluation of specific components of interventions (e.g., factorial designs). Thus, they are appropriate for addressing some specific needs in the evaluation of DHIs. However, it remains unsolved how to establish these alternative evaluation designs in research practice and how to deal with the limitations of the designs.
Differences in the Incidence of Adverse Events in Acute Care Hospitals: Results of a Multicentre Study
Authors: Jarošová Darja , Zeleníková Renáta , Plevová Ilona , Mynaříková Eva , Kachlová Miroslava
Published: April 2022

Background: Adverse events are indicators of patient safety and quality of care. Adverse events clearly have negative impacts on healthcare system costs. Organizational and unit characteristics are not very often studied in relation to adverse events. The aim of the study was to find the differences in the incidence of adverse events and healthcare-associated infections in hospitalized patients in Czech acute care hospitals according to type of hospital and type of unit. Methods: This cross-sectional multicentre study was conducted in 105 acute care medical and surgical units located in 14 acute care hospitals throughout the Czech Republic. The data on adverse events and healthcare-associated infections were reported monthly by nurse researchers. The data were collected from June 2020 to October 2020. Results: The incidence of healthcare-associated infections, pressure ulcers, and medication errors was significantly lower in large hospitals. Statistically significant differences have been further found between the incidence of pressure ulcers (<0.001), falls without injury (<0.001), and falls with injury (<0.001) in surgical and medical units. More pressure ulcers, falls without injury, and falls with injury have been reported in surgical units. Conclusion: The type of hospital and type of unit affected the incidence of adverse events at acute care hospitals. To reduce adverse events, a systematic adverse event measurement and reporting system should be promoted.
Chronic Exposure to Normobaric Hypoxia Increases Testosterone Levels and Testosterone/Cortisol Ratio in Cyclists
Authors: Czuba Miłosz , Płoszczyca Kamila , Kaczmarczyk Katarzyna , Langfort Józef , Gajda Robert
Published: April 2022

The aim of this study was to analyze the effects of the “live high, train low” method (LH–TL) and intermittent hypoxic training (IHT) on testosterone (T) and cortisol (C) levels in cyclists. Thirty cyclists participated in the experiment. The LH–TL group (n = 10) was exposed to normobaric hypoxia (FiO2 = 16.3%) for 11–12 h a day and trained in normoxia for 3 weeks. In the IHT group (n = 10), participants followed the IHT routine three times a week for 3 weeks in normobaric hypoxia (FiO2 = 16.3%). The control group (N; n = 10) followed the same training protocol in normoxia. The LH–TL training was found to significantly increase (p < 0.05) T levels and the testosterone/cortisol (T/C) ratio during the experiment. The area under the curve (AUC) calculated for T levels over 4 weeks was significantly (p < 0.05) higher in the LH–TL group, by 25.6%, compared to the N group. The results also indicated a significant correlation (r = 0.53; p < 0.05) between AUC for T levels over 4 weeks and ∆ values of hemoglobin (HGB) in the LH–TL group. Overall, the findings show that LH–TL training at a moderate simulated altitude contributes to an increase in T levels and T/C ratio in athletes, which is a beneficial change stimulating anabolic processes and erythropoiesis.
Intergenerational Taekwondo Program: A Narrative Review and Practical Intervention Proposal
Authors: Kim Yongseop , Kim Junhyoung , Lee Jung-Min , Seo Dong-Chul , Jung Hyun Chul
Published: April 2022

Taekwondo is a modernized martial art that includes various combinations of hand and kicking techniques and core values of Taekwondo philosophy such as courtesy, mutual respect, and self-control. Physical inactivity is highly prevalent among older adults and is a major contributor to health-related problems. Intergenerational physical activity programs are used as an effective tool to make a positive connection between generations and provide additional health benefits for both generations. This review study aimed to examine the theories of intergenerational physical activity programs and propose the Intergenerational Taekwondo Program (ITP). Various theories such as the transtheoretical model, contact theory, social capital theory, situated learning theory, human development theory, personality theory, and whole-person wellness model have been adopted in intergenerational physical activity programs. Our review suggests that to develop the Intergenerational Taekwondo Program, instructors should (1) establishing common goals, (2) understand differences in physical and mental abilities, and (3) offer incentives to encourage participants in physical activity programs. The proposed ITP program has the potential to not just provide unique inherent values and improving physical functions, but also to form generational connections.
Pain Management Program in Cardiology: A Template for Application of Normalization Process Theory and Social Marketing to Implement a Change in Practice Quality Improvement
Authors: Bode Kerstin , Whittaker Peter , Dressler Miriam , Bauer Yvonne , Ali Haider
Published: April 2022

Quality improvement plays a major role in healthcare, and numerous approaches have been developed to implement changes. However, the reasons for success or failure of the methods applied often remains obscure. Normalization process theory, recently developed in sociology, provides a flexible framework upon which to construct quality improvement. We sought to determine if examination of a successful quality improvement project, using normalization process theory and social marketing, provided insight into implementation. We performed a retrospective analysis of the steps taken to implement a pain management program in an electrophysiology clinic. We mapped these steps, and the corresponding social marketing tools used, to elements of normalization process theory. The combination of mapping implementation steps and marketing approaches to the theory provided insight into the quality-improvement process. Specifically, examination of the steps in the context of normalization process theory highlighted barriers to implementation at individual, group, and organizational levels. Importantly, the mapping also highlighted how facilitators were able to overcome the barriers with marketing techniques. Furthermore, integration with social marketing revealed how promotion of tangibility of benefits aided communication and how process co-creation between stakeholders enhanced value. Our implementation of a pain-management program was successful in a challenging environment composed of several stakeholder groups with entrenched initial positions. Therefore, we propose that the behavior change elements of normalization process theory combined with social marketing provide a flexible framework to initiate quality improvement.
Telemonitoring in Long-COVID Patients—Preliminary Findings
Authors: Romaszko-Wojtowicz Anna , Maksymowicz Stanisław , Jarynowski Andrzej , Jaśkiewicz Łukasz , Czekaj Łukasz , Doboszyńska Anna
Published: April 2022

The COVID-19 pandemic has revealed the high usefulness of telemedicine. To date, no uniform recommendations or diagnostic protocols for long-COVID patients have been developed. This article presents the preliminary results of the examination of patients after SARS-CoV-2 infection who were provided with medical telemonitoring devices in order to oversee their pulmonological and cardiological health. Three cases have been analyzed. Each patient underwent a 10-day registration of basic vital signs, in three 15-min sessions daily: RR (respiratory rate), ECG (electrocardiogram), HR (pulse), SPO2 (saturation), body temperature and cough. Rule methods and machine learning were employed to automatically detect events. As a result, serious disorders of all the three patients were detected: cardiological and respiratory disorders that required extended diagnostics. Furthermore, average values of the selected parameters (RR, HR, SPO2) were calculated for every patient, including an indication of how often they exceeded the alarm thresholds. In conclusion, monitoring parameters in patients using telemedicine, especially in a time of limited access to the healthcare system, is a valuable clinical instrument. It enables medical professionals to recognize conditions which may endanger a patient’s health or life. Telemedicine provides a reliable assessment of a patient’s health status made over a distance, which can alleviate a patient’s stress caused by long-COVID syndrome. Telemedicine allows identification of disorders and performing further diagnosis, which is possible owing to the implementation of advanced analysis. Telemedicine, however, requires flexibility and the engagement of a multidisciplinary team, who will respond to patients’ problems on an ongoing basis.
COVID-19 Clinical Profiles and Fatality Rates in Hospitalized Patients Reveal Case Aggravation and Selective Co-Infection by Limited Gram-Negative Bacteria
Authors: Said Kamaleldin , Alsolami Ahmed , Moussa Safia , Alfouzan Fayez , Bashir Abdelhafiz , Rashidi Musleh , Aborans Rana , Taha Taha , Almansour Husam , Alazmi Mashari , Al-Otaibi Amal , Aljaloud Luluh , Al-Anazi Basmah , Mohialdin Ahmed , Aljadani Ahmed
Published: April 2022

Bacterial co-infections may aggravate COVID-19 disease, and therefore being cognizant of other pathogens is imperative. We studied the types, frequency, antibiogram, case fatality rates (CFR), and clinical profiles of co-infecting-pathogens in 301 COVID-19 patients. Co-infection was 36% (n = 109), while CFR was 31.2% compared to 9.9% in non-co-infected patients (z-value = 3.1). Four bacterial species dominated, namely, multidrug-resistant Klebsiella pneumoniae (37%, n = 48), extremely drug-resistant Acinetobacter baumannii (26%, n = 34), multidrug-resistant Eschericia. coli (18.6%, n = 24), and extremely drug-resistant Pseudomonas aeruginosa (8.5%, n = 11), in addition to other bacterial species (9.3%, n = 12). Increased co-infection of K. pneumoniae and A. baumannii was associated with increased death rates of 29% (n = 14) and 32% (n = 11), respectively. Klebsiella pneumoniae was equally frequent in respiratory and urinary tract infections (UTI), while E. coli mostly caused UTI (67%), and A. baumannii and P. aeruginosa dominated respiratory infections (38% and 45%, respectively). Co-infections correlated with advance in age: seniors ≥ 50 years (71%), young adults 21–49 years (25.6%), and children 0–20 years (3%). These findings have significant clinical implications in the successful COVID-19 therapies, particularly in geriatric management. Future studies would reveal insights into the potential selective mechanism(s) of Gram-negative bacterial co-infection in COVID-19 patients.
Metabolic, Oxidative and Psychological Stress as Mediators of the Effect of COVID-19 on Male Infertility: A Literature Review
Authors: Mintziori Gesthimani , Duntas Leonidas H. , Veneti Stavroula , Goulis Dimitrios G.
Published: April 2022

Over 300 million patients with coronavirus disease 2019 (COVID-19) have been reported worldwide since the outbreak of the pandemic in Wuhan, Hubei Province, China. COVID-19 is induced by the acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The effect of SARS-CoV-2 infection on the male reproductive system is unclear. The aim of this review is to assess the effect of SARS-CoV-2 infection on male fertility and the impact of possible mediators, such as metabolic, oxidative and psychological stress. SARS-CoV-2 infection aggravates metabolic stress and directly or indirectly affects male fertility by reducing seminal health. In addition, SARS-CoV-2 infection leads to excessive production of reactive oxygen species (ROS) and increased psychological distress. These data suggest that SARS-CoV-2 infection reduces male fertility, possibly by means of metabolic, oxidative and psychological stress. Therefore, among other consequences, the possibility of COVID-19-induced male infertility should not be neglected.
Pharmacist Intention to Provide Medication Therapy Management Services in Saudi Arabia: A Study Using the Theory of Planned Behaviour
Authors: Alshehri Ahmed M. , Alenazi Omar S. , Almutairi Salman A. , Alali Ali Z. , Almogbel Yasser S. , Alonazi Rana E. , Alkhelaifi Hind A. , Alshehri Waleed M. , Alsehli Faisal A.
Published: April 2022

Medication therapy management (MTM) is provided by pharmacists and other healthcare providers, improves patient health status, and increases the collaboration of MTM providers with others. However, little is known about pharmacists’ intention to provide MTM services in Saudi Arabia. This study aimed to predict the pharmacists’ willingness in this nation to commit to providing MTM services there. This study used a cross-sectional questionnaire based on the theory of planned behaviour (TPB). The survey was distributed to 149 pharmacists working in hospital and community pharmacies. It included items measuring pharmacist attitudes, intentions, subjective norms, perceived behavioural control, knowledge about the provision of MTM services, and other sociodemographic and pharmacy practice-related items. The pharmacists had a positive attitude towards MTM services (mean = 6.15 ± 1.12) and strong intention (mean = 6.09 ± 1.15), highly perceived social pressure to provide those services (mean = 5.42 ± 1.03), strongly perceived control over providing those services (mean = 4.98 ± 1.05), and had good MTM knowledge (mean = 5.03 ± 1.00). Pharmacists who completed a pharmacy residency programme and had good knowledge of MTM services and a positive attitude towards them usually strongly intended to provide MTM services. Thus, encouraging pharmacists to complete pharmacy residency programmes and educating them about the importance and provision of MTM services will enhance their motivation to provide them.
The Post-Traumatic Stress Disorder Checklist for DSM-5: Psychometric Properties of the Italian Version
Authors: Di Tella Marialaura , Romeo Annunziata , Zara Georgia , Castelli Lorys , Settanni Michele
Published: April 2022

Background: The present study aimed to investigate the psychometric properties of the Italian version of the PTSD Checklist for the DSM-V (PCL-5) in a group of adults who had experienced heterogenous traumatic events. Methods: Six hundred and one participants met the inclusion criteria and completed a set of questionnaires through an online survey. Before administering the survey, the PCL-5 was translated into Italian according to the back-translation method. The factorial structure of the PCL-5 was assessed through multiple confirmatory factor analyses. Gender measurement invariance and concurrent and criterion validity were also evaluated. Results: The instrument had a seven-factor structure and it worked in a similar manner for males and females. With regard to the concurrent validity, results showed that higher PCL-5 scores were associated with higher levels of depression and rumination and lower levels of life satisfaction. Regarding criterion validity, results revealed that PCL-5 scores were, on average, higher for females than for males, and the temporal distance from the traumatic event was negatively correlated with the total PCL-5 score. Conclusions: The findings indicated that the Italian version of the PCL-5 was able to provide valid and reliable scores for the assessment of PTSD symptoms in the Italian population.
Occurrence of Microplastics in Tap and Bottled Water: Current Knowledge
Authors: Gambino Isabella , Bagordo Francesco , Grassi Tiziana , Panico Alessandra , De Donno Antonella
Published: April 2022

A narrative review was carried out to describe the current knowledge related to the occurrence of MPs in drinking water. The reviewed studies (n = 21) showed the presence of microplastics (MPs) in tap (TW) and bottled (BW) water, increasing concerns for public health due to the possible toxicity associated with their polymeric composition, additives, and other compounds or microorganism adsorbed on their surface. The MP concentration increase by decreasing particles size and was higher in BW than in TW. Among BW, reusable PET and glass bottles showed a higher MP contamination than other packages. The lower MP abundance in TW than in natural sources indicates a high removal rate of MPs in drinking water treatment plants. This evidence should encourage the consumers to drink TW instead of BW, in order to limit their exposure to MPS and produce less plastic waste. The high variability in the results makes it difficult to compare the findings of different studies and build up a general hypothesis on human health risk. A globally shared protocol is needed to harmonize results also in view of the monitoring plans for the emerging contaminants, including MPs, introduced by the new European regulation.
Pollution Indicators and HAB-Associated Halophilic Bacteria Alongside Harmful Cyanobacteria in the Largest Mussel Cultivation Area in Greece
Authors: Kalaitzidou Maria P. , Alvanou Maria V. , Papageorgiou Konstantinos V. , Lattos Athanasios , Sofia Marina , Kritas Spyridon K. , Petridou Evanthia , Giantsis Ioannis A.
Published: April 2022

Taking into consideration the essential contribution of Mytilus galloprovincialis farming, it is of rising importance to add knowledge regarding bacterial species occurrence in water samples from aquaculture zones from the point of view of both the organism and public health. In the present study, we investigated the bacterial community existing in water samples from six Mytilus galloprovincialis aquaculture areas in the Thermaikos gulf, northern Greece, that may provoke toxicity in aquatic organisms and humans and may indicate environmental pollution in mussel production as well as algal blooms. Bacterial species were identified molecularly by sequencing of a partial 16s rRNA segment and were analyzed phylogenetically for the confirmation of the bacterial taxonomy. The results obtained revealed the presence of four bacterial genera (Halomonas sp., Planococcus sp., Sulfitobacter sp., and Synechocystis sp.). Members of the Halomonas and Sulfitobacter genera have been isolated from highly polluted sites, Planococcus bacteria have been identified in samples derived directly from plastic debris, and Synechocystis bacteria are in line with microcystin detection. In this context, the monitoring of the bacteria community in mussel aquaculture water samples from the Thermaikos gulf, the largest mussel cultivation area in Greece, represents an indicator of water pollution, microplastics presence, algal blooms, and toxin presence.