bluuebunny/crossref_metadata_2025 huggingface dataset:
Phosphorus-solubilizing bacteria improve the growth of Nicotiana benthamiana on lunar regolith simulant by dissociating insoluble inorganic phosphorus
Authors: Xia Yitong , Yuan Yu , Li Chenxi , Sun Zhencai
Published: November 2023

In-situ utilization of lunar soil resources will effectively improve the self-sufficiency of bioregenerative life support systems for future lunar bases. Therefore, we have explored the microbiological method to transform lunar soil into a substrate for plant cultivation. In this study, five species of phosphorus-solubilizing bacteria are used as test strains, and a 21-day bio-improving experiment with another 24-day Nicotiana benthamiana cultivation experiment are carried out on lunar regolith simulant. We have observed that the phosphorus-solublizing bacteria Bacillus mucilaginosus, Bacillus megaterium, and Pseudomonas fluorescens can tolerate the lunar regolith simulant conditions and dissociate the insoluble phosphorus from the regolith simulant. The phosphorus-solubilizing bacteria treatment improves the available phosphorus content of the regolith simulant, promoting the growth of Nicotiana benthamiana. Here we demonstrate that the phosphorus-solubilizing bacteria can effectively improve the fertility of lunar regolith simulant, making it a good cultivation substrate for higher plants. The results can lay a technical foundation for plant cultivation based on lunar regolith resources in future lunar bases.
Vitamin B5 supports MYC oncogenic metabolism and tumor progression in breast cancer
Authors: Kreuzaler Peter , Inglese Paolo , Ghanate Avinash , Gjelaj Ersa , Wu Vincen , Panina Yulia , Mendez-Lucas Andres , MacLachlan Catherine , Patani Neill , Hubert Catherine B. , Huang Helen , Greenidge Gina , Rueda Oscar M. , Taylor Adam J. , Karali Evdoxia , Kazanc Emine , Spicer Amy , Dexter Alex , Lin Wei , Thompson Daria , Silva Dos Santos Mariana , Calvani Enrica , Legrave Nathalie , Ellis James K. , Greenwood Wendy , Green Mary , Nye Emma , Still Emma ,   CRUK Rosetta Grand Challenge Consortium, Kreuzaler Peter , Barry Simon , Goodwin Richard J. A. , Bruna Alejandra , Caldas Carlos , MacRae James , de Carvalho Luiz Pedro Sório , Poulogiannis George , McMahon Greg , Takats Zoltan , Bunch Josephine , Yuneva Mariia
Published: November 2023

Tumors are intrinsically heterogeneous and it is well established that this directs their evolution, hinders their classification and frustrates therapy1–3. Consequently, spatially resolved omics-level analyses are gaining traction4–9. Despite considerable therapeutic interest, tumor metabolism has been lagging behind this development and there is a paucity of data regarding its spatial organization. To address this shortcoming, we set out to study the local metabolic effects of the oncogene c-MYC, a pleiotropic transcription factor that accumulates with tumor progression and influences metabolism10,11. Through correlative mass spectrometry imaging, we show that pantothenic acid (vitamin B5) associates with MYC-high areas within both human and murine mammary tumors, where its conversion to coenzyme A fuels Krebs cycle activity. Mechanistically, we show that this is accomplished by MYC-mediated upregulation of its multivitamin transporter SLC5A6. Notably, we show that SLC5A6 over-expression alone can induce increased cell growth and a shift toward biosynthesis, whereas conversely, dietary restriction of pantothenic acid leads to a reversal of many MYC-mediated metabolic changes and results in hampered tumor growth. Our work thus establishes the availability of vitamins and cofactors as a potential bottleneck in tumor progression, which can be exploited therapeutically. Overall, we show that a spatial understanding of local metabolism facilitates the identification of clinically relevant, tractable metabolic targets.
Spatial and single-cell profiling of the metabolome, transcriptome and epigenome of the aging mouse liver
Authors: Nikopoulou Chrysa , Kleinenkuhnen Niklas , Parekh Swati , Sandoval Tonantzi , Ziegenhain Christoph , Schneider Farina , Giavalisco Patrick , Donahue Kat-Folz , Vesting Anna Juliane , Kirchner Marcel , Bozukova Mihaela , Vossen Christian , Altmüller Janine , Wunderlich Thomas , Sandberg Rickard , Kondylis Vangelis , Tresch Achim , Tessarz Peter
Published: November 2023

Tissues within an organism and even cell types within a tissue can age with different velocities. However, it is unclear whether cells of one type experience different aging trajectories within a tissue depending on their spatial location. Here, we used spatial transcriptomics in combination with single-cell ATAC-seq and RNA-seq, lipidomics and functional assays to address how cells in the male murine liver are affected by age-related changes in the microenvironment. Integration of the datasets revealed zonation-specific and age-related changes in metabolic states, the epigenome and transcriptome. The epigenome changed in a zonation-dependent manner and functionally, periportal hepatocytes were characterized by decreased mitochondrial fitness, whereas pericentral hepatocytes accumulated large lipid droplets. Together, we provide evidence that changing microenvironments within a tissue exert strong influences on their resident cells that can shape epigenetic, metabolic and phenotypic outputs.
Analysis of microbial composition and sharing in low-biomass human milk samples: a comparison of DNA isolation and sequencing techniques
Authors: Spreckels Johanne E , Fernández-Pato Asier , Kruk Marloes , Kurilshikov Alexander , Garmaeva Sanzhima , Sinha Trishla , Ghosh Hiren , Harmsen Hermie , Fu Jingyuan , Gacesa Ranko , Zhernakova Alexandra
Published: November 2023

Human milk microbiome studies are currently hindered by low milk bacterial/human cell ratios and often rely on 16S rRNA gene sequencing, which limits downstream analyses. Here, we aimed to find a method to study milk bacteria and assess bacterial sharing between maternal and infant microbiota. We tested four DNA isolation methods, two bacterial enrichment methods and three sequencing methods on mock communities, milk samples and negative controls. Of the four DNA isolation kits, the DNeasy PowerSoil Pro (PS) and MagMAX Total Nucleic Acid Isolation (MX) kits provided consistent 16S rRNA gene sequencing results with low contamination. Neither enrichment method substantially decreased the human metagenomic sequencing read-depth. Long-read 16S-ITS-23S rRNA gene sequencing biased the mock community composition but provided consistent results for milk samples, with little contamination. In contrast to 16S rRNA gene sequencing, 16S-ITS-23S rRNA gene sequencing of milk, infant oral, infant faecal and maternal faecal DNA from 14 mother-infant pairs provided sufficient resolution to detect significantly more frequent sharing of bacteria between related pairs compared to unrelated pairs. In conclusion, PS or MX kit-DNA isolation followed by 16S rRNA gene sequencing reliably characterises human milk microbiota, and 16S-ITS-23S rRNA gene sequencing enables studies of bacterial transmission in low-biomass samples.
The grade of individual prostate cancer lesions predicted by magnetic resonance imaging and positron emission tomography
Authors: Nilsson Erik , Sandgren Kristina , Grefve Josefine , Jonsson Joakim , Axelsson Jan , Lindberg Angsana Keeratijarut , Söderkvist Karin , Karlsson Camilla Thellenberg , Widmark Anders , Blomqvist Lennart , Strandberg Sara , Riklund Katrine , Bergh Anders , Nyholm Tufve
Published: November 2023

Background

Multiparametric magnetic resonance imaging (mpMRI) and positron emission tomography (PET) are widely used for the management of prostate cancer (PCa). However, how these modalities complement each other in PCa risk stratification is still largely unknown. We aim to provide insights into the potential of mpMRI and PET for PCa risk stratification.

Methods

We analyzed data from 55 consecutive patients with elevated prostate-specific antigen and biopsy-proven PCa enrolled in a prospective study between December 2016 and December 2019. [68Ga]PSMA-11 PET (PSMA-PET), [11C]Acetate PET (Acetate-PET) and mpMRI were co-registered with whole-mount histopathology. Lower- and higher-grade lesions were defined by International Society of Urological Pathology (ISUP) grade groups (IGG). We used PET and mpMRI data to differentiate between grades in two cases: IGG 3 vs. IGG 2 (case 1) and IGG ≥ 3 vs. IGG ≤ 2 (case 2). The performance was evaluated by receiver operating characteristic (ROC) analysis.

Results

We find that the maximum standardized uptake value (SUVmax) for PSMA-PET achieves the highest area under the ROC curve (AUC), with AUCs of 0.72 (case 1) and 0.79 (case 2). Combining the volume transfer constant, apparent diffusion coefficient and T2-weighted images (each normalized to non-malignant prostatic tissue) results in AUCs of 0.70 (case 1) and 0.70 (case 2). Adding PSMA-SUVmax increases the AUCs by 0.09 (p < 0.01) and 0.12 (p < 0.01), respectively.

Conclusions

By co-registering whole-mount histopathology and in-vivo imaging we show that mpMRI and PET can distinguish between lower- and higher-grade prostate cancer, using partially discriminative cut-off values.
Understanding intracranial aneurysm sounds via high-fidelity fluid-structure-interaction modelling
Authors: Bruneau David A. , Steinman David A. , Valen-Sendstad Kristian
Published: November 2023

Background

Since the 1960s, the origins of intracranial aneurysm bruits and musical murmurs have been debated, with proposed mechanisms ranging from self-excitation (i.e., resonance) by stable pulsatile flow, to vibration caused by unstable (laminar vortex shedding or turbulent) flow. This knowledge gap has impeded the use of intracranial sounds a marker of aneurysm remodelling or rupture risk. New computational techniques now allow us to model these phenomena.

Methods

We performed high-fidelity fluid-structure interaction simulations capable of understanding the magnitude and mechanisms of such flow-induced vibrations, under pulsatile flow conditions. Six cases from a previous cohort were used.

Results

In five cases, underlying flow instabilities present as broad-band, random vibrations, consistent with previously-described bruits, while the sac also exhibits resonance, rocking back and forth in different planes of motion, consistent with previously described musical murmurs. Both types of vibration have amplitudes in the range of 0.1 to 1 μm. The murmurs extend into diastole, after the underlying flow instability has dissipated, and do not exhibit the characteristic repeating frequency harmonics of previously hypothesized vortex-shedding mechanisms. The remaining case with stable pulsatile flow does not vibrate. Spectrograms of the simulated vibrations are consistent with previously reported microphone and Doppler ultrasound recordings.

Conclusions

Our results provide a plausible explanation for distinct intracranial aneurysm sounds and characterize the mechanical environment of a vibrating aneurysm wall. Future work should aim to quantify the deleterious effects of these overlooked stimuli on the vascular wall, to determine which changes to the wall makeup are associated with vibration.
A dual channel fluorescence tongue for catechin recognition based on the MnO2 nanorods–Amplex Red–o-phenylenediamine reaction system
Authors: Liu Chang , Qian Zhenni , Chen Zhengbo
Published: November 2023

Here, we report a dual-channel fluorescence sensor array for catechin discrimination based on the MnO2 nanorods (NRs)–Amplex Red (AR)–o-phenylenediamine (OPD) catalytic reaction system.
Simply accessible platinum(ii) complexes enabling alkene hydrosilylation at ppm catalyst loadings
Authors: Maliszewski Benon P. , Casillo Eleonora , Lambert Perrine , Nahra Fady , Cazin Catherine S. J. , Nolan Steven P.
Published: November 2023

An efficient olefin hydrosilylation protocol utilising Pt(ii)–thioether-based pre-catalysts is reported.
Correlation of magnetic resonance (EPR, ssNMR) parameters and crystal-microstrain in marbles as a tool to probe their provenance
Authors: Belles Loukas , Dimitriou Christos , Moularas Constantinos , Solakidou Maria , Theorodakopoulos Marinos , Louloudi Maria , Deligiannakis Yiannis
Published: November 2023

The provenance of marbles can be probed, based on correlation between the EPR parameter (D) of Mn2+-ions in marbles, and lattice-macrostrain (ε0) of marble crystal, obtained by high-resolution XRD.
Hole-transporting interlayers based on pyrazine-containing conjugated polymers for perovskite solar cells
Authors: Zamoretskov D. S. , Kuznetsov I. E. , Zhivchikova A. N. , Tepliakova M. M. , Sagdullina D. K. , Gapanovich M. V. , Kurbatov V. G. , Nasibulin A. G. , Akkuratov A. V.
Published: November 2023

Pyrazine-based conjugated polymers are shown as promising hole-transport materials for perovskite solar cells with encouraging efficiency and high operational stability.
Enhancement of wide-band trace terahertz absorption spectroscopy based on microstructures: a review
Authors: Yan Dexian , Cui Jing , Li Xiangjun , Zhang Le , Li Jining , Lu Wenxin
Published: November 2023

Recent investigations into terahertz molecular fingerprint spectrum enhancement strategies in microstructures are reviewed.
Noble metal-free bimetallic phosphide-decorated Zn0.5Cd0.5S with efficient photocatalytic H2 evolution
Authors: Chen Lu , Chen Feng , Xia Yuzhou , Liang Ruowen , Huang Renkun , Yan Guiyang , Ying Shaoming
Published: November 2023

The 7% NiCoP/Zn0.5Cd0.5S photocatalyst exhibits a remarkable photocatalytic hydrogen evolution rate of 19.5 mmol g−1 h−1, which is 27 times higher than that of pristine Zn0.5Cd0.5S.
Rare three-valence-band convergence leading to ultrahigh thermoelectric performance in all-scale hierarchical cubic SnTe
Authors: Li Fan , Liu Xin , Li Shu-Rong , Zhang Xiao-Fan , Ma Ni , Li Xin-Jing , Lin Xin-Yun , Chen Ling , Wu Haijun , Wu Li-Ming
Published: November 2023

Three-valence-band (i.e., L, Σ and Λ) charge transport and multiple-scale defects were simultaneously achieved in p-type SnTe through dual incorporation of MnCdTe2 and Ge, which contributed to a record-high ZT of ∼1.97 at 900 K.
Bio-glycerol hydrodeoxygenation to propylene: advancing knowledge on Mo-based catalyst characteristics and reaction pathways under flow conditions
Authors: Ioannidou Georgia , Lemonidou Angeliki A.
Published: November 2023

In this work, the reaction pathways of one-step glycerol hydrodeoxygenation in the gas phase are exploited under flow conditions over molybdena-based catalysts (8.7 wt% Mo/black carbon).
Surfactant-assisted synthesis of zero-dimensional iron nanomaterial for cellobiose hydrolysis
Authors: Singh Hari , Sinha Anil Kumar , Kour Sharanmeet , Barheyan Suneel Singh , Goel Gaurav , Mishra Jibanananda
Published: November 2023

m-FeCTB was synthesized by chemical precipitation. This work reports a cost-effective nanomaterial with surface acidity of 26 μmol g−1, high crystallinity, ferromagnetic and catalytically active for the hydrolysis of biomass-derived cellobiose to glucose and HMF.
Combined three dimensional locomotion and deformation of functional ferrofluidic robots
Authors: Fan Xinjian , Zhang Yunfei , Wu Zhengnan , Xie Hui , Sun Lining , Chen Tao , Yang Zhan
Published: November 2023

Magnetic-controlled ferrofluidic robots demonstrate exceptional precision in efficiently navigating complex 3D paths, which enhances their applicability across diverse fields, including medicine, micromanipulation, and bioengineering.
A supramolecular assembly-based strategy towards the generation and amplification of photon up-conversion and circularly polarized luminescence
Authors: Sengupta Alisha , Roy Gargee , Likhar Aakash Ravikant , Asthana Deepak
Published: November 2023

For the molecular properties in which energy transfer/migration is determinantal, such as triplet–triplet annihilation-based photon up-conversion, intermolecular distances and relative molecular orientations play pivotal roles.
A moderate method for in situ growing Fe-based LDHs on Ni foam for catalyzing the oxygen evolution reaction
Authors: Liu Yanqi , Zhang Chenghao , Cai Qingsong , Zhang Jianmin , Zheng Zongmin
Published: November 2023

A moderate method for in-situ growing Fe-based LDHs on Ni foam by using oxidation and hydrolysis reactions of Fe2+ at room temperature. The NiFe-LDH@NF exhibits excellent OER performance with an overpotential of only 206 mV at a current density of 10 cm−2 in 1 M KOH.
Advances in CO2 activation by frustrated Lewis pairs: from stoichiometric to catalytic reactions
Authors: Khan Md. Nasim , van Ingen Yara , Boruah Tribani , McLauchlan Adam , Wirth Thomas , Melen Rebecca L.
Published: November 2023

Frustrated Lewis pair systems have been explored efficiently in homogeneous and heterogeneous conditions for the activation and reduction of CO2 to various useful products in stoichiometric as well as in catalytic reactions.
A guide to lignin valorization in biorefineries: traditional, recent, and forthcoming approaches to convert raw lignocellulose into valuable materials and chemicals
Authors: Brienza Filippo , Cannella David , Montesdeoca Diego , Cybulska Iwona , Debecker Damien P.
Published: November 2023

This tutorial review aims at providing a complete overview of the strategies for the conversion of lignocellulose in current and future biorefineries, with a particular focus on the transformation of lignin toward valuable products.
Elucidating the interaction of C-terminal domain of Vaccinia-Related Kinase 2A (VRK2A) with B-cell lymphoma-extra Large (Bcl-xL) to decipher its anti-apoptotic role in cancer
Authors: Puja Rashmi , Dutta Shubhankar , Bose Kakoli
Published: November 2023

Vaccinia-Related Kinase 2 (VRK2) is an anti-apoptotic Ser/Thr kinase that enhances drug sensitivity in cancer cells. This protein exists in two isoforms: VRK2A, the longer variant, and VRK2B, which lacks the C-terminal region and transmembrane domain. While the therapeutic importance of VRK2 family proteins is known, the specific roles of VRK2A and its interplay with apoptotic regulator Bcl-xL (B-cell lymphoma-extra Large) remain elusive. Bcl-xL regulates cell death by interacting with BAX (B-cell lymphoma-2 Associated X-protein), controlling its cellular localization and influencing BAX-associated processes and signaling pathways. As VRK2A interacts with the Bcl-xL–BAX complex, comprehending its regulatory engagement with Bcl-xL presents potential avenues for intervening in diseases. Using a multi-disciplinary approach, this study provides information on the cellular localization of VRK2A and establishes its interaction with Bcl-xL in the cellular milieu, pinpointing the interacting site and elucidating its anti-apoptotic property within the complex. Furthermore, this study also put forth a model that highlights the importance of VRK2A in stabilizing the ternary complex, formed with Bcl-xL and BAX, thereby impeding BAX dissociation and hence apoptosis. Therefore, further investigations associated with this important revelation will provide cues for designing cancer therapeutics in the future.
Blockchain privacy: Fundamental aspects and challenges for the future Internet data sharing
Authors: Kapsoulis Nikolaos , Psychas Alexandros , Litke Antonios , Varvarigou Theodora
Published: November 2023

Privacy concerns the majority of individuals, governments, and organizations that share data over dissimilar networks of nodes. Every kind of participant requires awareness of the data journey that unfolds inside a blockchain network with its own trustworthy rules of data management and accessibility. This paper provides a methodological approach on privacy for data sharing within blockchain environments. Specific technological aspects of blockchains that relate to on‐chain privacy such as network nature, party join, smart contracts, blockchain states, transactions, and ledger flows, are analysed with respect to the involvement and impact of privacy in distributed ledgers. A high‐level architectural approach is suggested that is intended to address significant privacy concerns on data sharing among different kinds of users in the context of blockchain networks deployment and the broader Web 3.0 ecosystem building. Simultaneously, many pertinent challenges are discussed regarding network nature, configurable privacy, ownership, confidentiality, secured computation, and data monetization that appear in the field and ought to be carefully tackled for the future mass adoption of privacy‐matured distributed ledgers that interconnect delivering the future Internet.
Electromagnetic analysis of moving structures in a moving reference frame
Authors: Marvasti M. , Boutayeb H.
Published: November 2023

In this work, the finite‐difference time‐domain (FDTD) method is employed to study electromagnetic problems with moving bodies in a moving system. The proposed approach consists in modeling objects with time‐varying positions and using the direct discretization of Maxwell's equations in space and time domains. Doppler effects are investigated for problems with moving observer, source, or reflector, in a moving frame. A distinction is also made between a high‐impedance or low‐impedance plane wave source in motion. The full‐wave electromagnetic simulations are compared with closed‐form equations that agree with wave theory. The proposed analysis shows that, for Doppler radars used every day, the motion of the Earth relative to the Cosmic Microwave Background has a negligible effect and only relative motions in the Earth frame are relevant.
The stock status of Lutjanus gibbus (Forsskal, 1775) in Kupang waters, East Nusa Tenggara
Authors: Panggabean Anthony Sisco , Prihatiningsih  , Taufik M. , Nurdin E. , Mahulette R.T. , Nurulluddin  , Chodrijah Umi , Pane A.R.
Published: November 2023

Lutjanus gibbus is a commercially valuable demersal fish and a potential fish resource in the waters of Kupang. Morphometrics data were collected from 3,890 specimens landed at PPI Oeba from March 2019 through December 2020—analysis of data including population size structure, dynamics, mortality, and exploitation rate. Population dynamics, growth, and exploitation rates were analyzed using the FiSAT II and ELEFAN software programs. The most common fishing gear is a handline and a bottom longline used in one-day fishing. The results showed that the catch length was between 18 - 50 cm TL. The value of Lc (33,1 cmTL) was greater than that of Lm (28,66 cmTL). The growth parameter values showed asymptotic length (L) 52.50 cmTL, growth rate (K) 0.31 per year, and length at time zero (t0) -0.45. The natural mortality rate (M) of 1.18 exceeded the fishing mortality rate (F) of 0.87, the total mortality rate (Z) of 2.05, and the exploitation rate (E) of 0.42. The exploitation rate of Lutjanus gibbus in these waters indicates that the stock condition is below the optimal limit (underexploited) so that it can still be exploited with an additional effort equal to 16% of the current attempt while maintaining sustainability.
Sea bunnies as a potential marine ecotourism in sumberkima, Buleleng Regency, Bali
Authors: Ampou Eghbert Elvan , Triyulianti Iis , Pradisty Novia Arinda , Widagti Nuryani , Swastana I. Gede Adi , Mariasa I. Putu Mangku
Published: November 2023

The Sumberkima Village has a vital coral reef ecosystem since the ecosystem has become home to some incredible marine biotas, including sea bunnies, a sea slug or Nudibranch species. The Nudibranch community can indicate the health of the ecosystem from its diversity and structure. This study aims to determine the distribution and value of the Nudibranchia Ecological Index and provide an example informative infographic of sea bunnies to support marine ecotourism at Sumberkima Village, Gerokgak District, Buleleng Regency, Bali Province. The research method applied is observation by visual census with an underwater camera (underwater visual census) and SCUBA diving equipment at a depth range of 5-25 meters. Observation data were collected in April 2021. A total of 15 species were found at ten dive sites.: Chromodoris annae, Chromodoris magnifica, Goniobranchus reticulatus, Hypselodoris apolegma, Hypselodoris bullockii, Nembrotha cristata, Nembrotha kubaryana, Nembrotha sp, Notodoris serenae, Phyllidia elegans, Phyllidia varicosa, Phyllidiopsis pipecki, Phyllidiopsis shireenae. The most dominant species was a member of Phyllidia genus, and an uncommon species is Goniobranchus reticulatus.
Efficacy of dietary engkabang oil (Shorea macrophylla) on growth performance of fingerling malayan mahseer (Tor tambroides)
Authors: Redhwan Aidil Ikhwan , Hock Sim Chun , Kari Asmad , Nguang Siew Ing , Ha Hou Chew , Komilus Connie Fay
Published: November 2023

The use ofShorea macrophyllaoil (SMO) as a substitute for fish oil in enhancing the growth performance of Malayan Mahseer (Tor tambroides) has received limited attention. This study aimed to evaluate the proximate composition fatty acid profiles of SMO and assess its impact as an alternative lipid source for replacing fish oil in the diet ofTor tambroides. A 2-month feeding trial was conducted using 75 fingerlings, averaging 17.9±0.1 g in weight, distributed randomly across five triplicated feed treatments. These dietary treatments consisted of 0% SMO (control), 1.25% SMO (T-1.25smo), 2.5% SMO (T-2.5smo), 3.75% SMO (T-3.75smo), and 5% SMO (T-5smo). The fatty acid composition ofShorea macrophyllaoil and the proximate composition of the treatment diets were analyzed. Growth indices (BWG, FI, FCR, and SR) were recorded at 10-day intervals. The results indicate that the incorporation of 2.5% SMO led to an 80.89% increase in BWG with the lowest FCR (2.61) and feed intake. This suggests that including 2.5%Shorea macrophyllaoil in the diet may positively impact the growth performance ofTor tambroidesfingerlings.
Forming the base population of fast-growing African catfish (Clarias gariepinus) carrying major histocompatibility complex class I (MHC I) allele through a within-family selection
Authors: Iswanto Bambang , Marnis Huria , Suprapto Rommy , Imron
Published: November 2023

African catfish (Clarias gariepinus) is one of the economically important species in Indonesian aquaculture that has experienced genetic deterioration. Thus, it should be genetically improved, mainly on the growth and disease resistance. The genetic improvement of those traits was initiated by forming the base population. The base population consisted of two populations, each resulting from a factorial mating design of a 2:2 ratio between 30 males and 30 females of Mutiara African catfish carrying major histocompatibility complex class I (MHC I) allele number 7. Forming the first base population resulted in 50 families, whereas the second resulted in 57 families. The potency of the base population in forming the next generation was then evaluated by estimating the genetic parameters, i.e., heritability, selection differential, selection intensity, and response to selection. Results of the estimation of genetic parameters for its body weight suggested that the heritability of both base populations was relatively high (0.33 and 0.44), with relatively low selection differential (28.07±11.39% and 23.78±7.42%) and selection intensity (1.13±0.22 and 1.13±0.19). The response to selection for body weight in the next generation was estimated at around 9.31-10.38%. Those results suggested that both base populations could be used in forming the next generation.
Nursery of sand lobster, Panulirus homarus (Linnaeus 1758) in rearing tanks with some shelter combinations
Authors: Slamet Bejo , Fahrudin  , Sudewi  , Astuti Widia , Giri Adiasmara
Published: November 2023

The addition of the right combination of shelters to lobster farming can reduce cannibalism and increase survival rates. This study aims to find a suitable shelter combination for Scalloped spiny lobster reared in a fiberglass tank. The study was conducted in 12 fiberglass tanks 1 m3 in volume, with an initial lobster puerulus weight of 0.26 ± 0.024 g and a density of 100 /tanks. The combination of shelters tested is roster brick (A); shading net (B); roster brick and seaweed Gracillaria sp. (C); and shading net and seaweed (D). The parameters observed were survival rate, body length, body weight and hemocytes. Results showed that the best survival rate at treatment C (36.3 ± 10.54%), followed by A (33.3 ± 5.77%), D (25.0 ± 5.57), and lowest at B (14,3 ± 4,04%) (P<0.05). The final body weight and total length did not significantly different (p>0.05). The highest total hemocytes at treatment C (616.5±67.254 cell/ml), followed by A (583.4± 59.434 cell/ml), D (572.6±56.324 cell/ml), and lowest at B (459.6±45.344 cell/ml). The shelter combination of roster brick and seaweed (Gracillaria sp.) is better than another treatment because it can produce a better survival rate.
Development of freeze-dried bivalent fish vaccine product with skimmed milk as stabiliser
Authors: Sugiani Desy , Lusiastuti Angela Mariana , Sumiati Tuti , Setiadi Setiadi , Purwaningsih Uni , Gardenia Lila , Taukhid  , Murwantoko  , Isnansetyo Alim , Handayani Desy Putri
Published: November 2023

Bacterial vaccines have been tested for their effectiveness in protecting fish from potential virulent diseases. The current vaccine preparations are mostly in liquid form, which affects prices due to an increase in the cost of shipment, the stability of liquid products also has limitations in long-term storage. This study aims to develop a method of preparation of bivalent vaccine through freeze dry method with skimmed milk as a filler for optimization of the vaccine products. The preparation of bivalent vaccine by freeze dry method can reduce 96.67% of total weight compared to vaccines in liquid form. The quality test showed that bivalent vaccine products by freeze dry method with skimmed milk filler containing bacteriaAeromonas hydrophilaandStreptococcus agalactiaeare passed the sterility and viability test. The application of the vaccine can provide a positive response to increase the antibody titer and other hematological parameter responses and increase the survival rate of fish. The development of freeze-dried vaccine products can optimize fish vaccines as a recommended product in fish disease management.
Feeding management and appraisal of recirculating aquaculture system for spiny lobster Panulirus spp. weaning
Authors: Sahidhir Ibnu , Syahrul  , Syafrizal  , Dewiyanti Irma , Jones Clive M. , Rimmer Michael A.
Published: November 2023

The study aimed to assess lobster fry adaptation to a formulated diet over 1-3 days while evaluating the recirculating system's performance. 1,500 pueruli lobsters (average weight: 0.31 ± 0.01 g) were placed in 30 round 80 L tanks for 30 days. Lobsters underwent various feeding strategies: formulated diet only (FD), adjusting 10% fresh diet per day (D1), every 2 days (D2), every 3 days (D3), and fresh diet only (Fr). Each treatment had five replicates. Results showed feeding strategies influenced lobster growth, with survival rates between 23%-29%. The recommended strategy was adjusting 10% fresh diet every three days, yielding the best performance in final weight (0.93 g), weight gain (187%), and specific growth rate (3.5% per day). Initial weeks saw high mortality and moulting during the puerulus to post-puerulus transition. Water quality analysis indicated the phytobiological filter had lower total ammonia nitrogen (TAN), nitrite-N, nitrate-N, and higher pH compared to other filters in use.
Determination of withdrawal time, efficacy, and safety test of enrofloxacin in carp (Cyprinus carpio)
Authors: Bond Manja Meyky , Senggagau Betutu , Anshori Sofian
Published: November 2023

The use of antibiotic such as enrofloxacin for the treatment of fish diseases needs to be appropriately controlled so that the medicine function effectively and does not cause antibiotic residues above the maximum residues limit (MRL). The study aims to determine the duration of administration, efficacy, and safety of enrofloxacin in carp. The size of the carp used was 10-40 g, with a length of 8-12 cm. The methods used in this study were (a) withdrawal time test, (b) efficacy and safety test, and (c) histopathology analysis. The results of determining enrofloxacin withdrawal time for 0.5 MRL and 1 MRL were two days and eight days, respectively. The survival rates of carp in post-challenge with Aeromonas hydrophila for the positive control and efficacy groups were 86.7% and 88.9%, respectively, and were not significantly different. The results of histopathological observations on the liver, spleen, kidney, and muscle of the carp showed no significant pathological changes between the safety test dose treatment and the control treatment.
Growth of Eucheuma cottonii seaweed from tissue culture with different time acclimatization in Hurun Bay
Authors: Meiyana Maya , Dhoe Silfester Basi , Minjoyo Herno , Rivaie Arief Rahman
Published: November 2023

Constraints on the seaweed culture in Indonesia until now still rely on seeds obtained by vegetative propagation and have poor quality, which often causes a decrease in genetic variability. Planting seaweed seedlings from tissue cultures that have been acclimatized in a greenhouse laboratory is believed to improve the quality of the seaweed seeds. This research aimed to observe the growth of tissue culture acclimatized Eucheuma cottonii seaweed seedlings to different rearing times in greenhouses applied to floating raft cages. This study used a completely randomized design (CRD) with three treatments and three replications. Acclimatization treatment was conducted for 2 weeks, 4 weeks, and 6 weeks. Seaweed seedlings acclimatized in the greenhouse are reared on bamboo floating rafts in the sea, with an initial weight of 5 grams each. The length of maintenance at sea is 28 days. The resulting daily growth rate in two-week acclimatization was 2.92%, the four-week treatment was 2.93%, and the six-week treatment was 2.57%. in raising seedlings at sea, the highest daily growth rate value was in the four-week treatment of 7.25%, followed by the two-week treatment of 7.11% and the six-week treatment of 6.43%.
Modeling roll contact curves of a squeezing machine
Authors: Sulaymanova Dildora , Abduganieva Yulduzoy , Miratoev Zokhidjon
Published: November 2023

Mathematical models of roll contact curves of leather squeezing machines were developed. The phenomenon of contact interaction of leather with working rolls is considered taking into account the water filtration during the squeezing process and the strain properties of leather and cloth. Analysis of the obtained models showed that the contact curves of the rolls depend on the thickness, humidity and deformation properties of the leather, the radii of the rolls and the deformation properties of the cloth, as well as on the forces acting on their supports.
Increasing the efficiency of heat exchange by changing the construction of a shell-and-tube heat exchanger
Authors: Rakhimov Ganisher B. , Murtazayev Feruzbek I.
Published: November 2023

To date, various methods of intensifying convective heat transfer have been proposed and studied. This article focuses on solutions to increase the heat exchange efficiency and maintenance time of the shell-and-tube heat exchanger by preventing the formation of liquid vapors in the internal pipes and distribution chamber of the device when the liquid flow moves through the internal pipes.
Analysis of the optimization of labor protection measures in engineering work
Authors: Kurbonov A.J. , Yuldoshev O.R. , Saidxonova N.J. , Oblakulov S.T. , Djumakulova K.A.
Published: November 2023

The paper is devoted to the analysis of the extent of injury in the coal mine by age categories of employees. Different age groups of employees are studied. Measures taken to protect workers by types of productive activities in coal mines aimed at improving working conditions in the workplace are proposed. Due to the proposed measures the decrease of level of injury and occupational diseases of workers was achieved.
Collection and sorting of glass waste for recycling in the production of building materials and products
Authors: Shchepochkina Julia , Klyosov Dmitry , Matyusheva Nadezhda , Zyryanov Vladimir , Nurullin Aidar
Published: November 2023

The problems of collecting and sorting glass waste at the place of their formation, extraction of waste from dumps are considered. The sorting of glass waste according to the principle of the former purpose of the product made from it is proposed. It is proposed to divide all glass waste collected or extracted from dumps into ten groups for subsequent processing in the production of a wide range of building materials and products.
Prospects and tasks of sound vision application for diagnostics and visualization of cavitation and turbulent flows in medium and large diameter fittings
Authors: Gorobchenko Stanislav , Kovalev Dmitriy , Taraban Maria , Meshkov Sergey , Bedenko Iraida , Sakhapov Rustem
Published: November 2023

The possibility of using sound vision to solve the problems of visualization and diagnostics of caviation and turbulent flows arising during the operation of pipeline valves is considered. The available methods of realization of sound vision are analyzed. A block diagram with the reconstruction of a holographic image is proposed. The diagram could visualize the picture of the cavitation flow. It is shown that it is most promising to develop such circuit and technical solutions that could form a complete picture of the flow in dynamics with the ability to digitize it for further analysis.
Analysis of desertification trends in Central Asia based on MODIS Data using Google Earth Engine
Authors: Aslanov Ilhomjon , Teshaev Nozimjon , Khayitov Kholmurod , Mukhtorov Uzbekkhon , Khaitbaeva Jamila , Murodova Dilrabo
Published: November 2023

Desertification is a significant environmental issue affecting arid and semi-arid regions globally, including Central Asia. Monitoring and analyzing desertification trends is crucial for understanding the extent of land degradation and implementing effective management strategies. This literature review aims to provide an overview of existing research on analyzing desertification trends in Central Asia using MODIS data and the application of Google Earth Engine for analysis. Remote Sensing and Desertification Monitoring: Remote sensing techniques, particularly those utilizing satellite data, have been widely employed for monitoring desertification processes. The Moderate Resolution Imaging Spectroradiometer (MODIS) sensor onboard various NASA satellites provides valuable data for assessing vegetation dynamics and land cover changes associated with desertification. Central Asia and Desertification: Central Asia, encompassing countries such as Kazakhstan, Uzbekistan, Turkmenistan, Kyrgyzstan, and Tajikistan, faces significant desertification challenges. Studies have highlighted the impacts of climate change, unsustainable land management practices, and population growth on desertification in the region. Monitoring and analyzing desertification trends in Central Asia are essential for developing targeted mitigation and adaptation strategies.
L’anesthésie, un processus commun à tout le vivant
Authors: Sylvain-Bonfanti Lucia , Page Julien , Arbelet-Bonnin Delphine , Meimoun Patrice , Grésillon Étienne , Bouteau François , Laurenti Patrick
Published: November 2023

Du fait de leur intérêt en médecine, la majeure partie des études actuelles sur les anesthésiques se concentrent sur le système nerveux des animaux et négligent le fait que toute forme de vie peut être anesthésiée. En effet, l’anesthésie cible des canaux dépendants du voltage, canaux qui existent dans un grand nombre d’espèces diverses et qui proviennent de canaux ancestraux antérieurs à l’apparition même des eucaryotes. La question demeure : le maintien au cours de l’évolution de la capacité à être anesthésié est-il dû à un avantage adaptatif ou à un simple défaut intrinsèque des canaux ioniques ? Le regain d’intérêt actuel pour les modèles non animaux ouvre l’espoir non seulement de découvrir de nouvelles molécules anesthésiantes, mais aussi de progresser dans notre connaissance fondamentale de ce phénomène encore mal compris.
Telemedizin, Kratom und
Autismus-Spektrum-Störung
Authors: Mulert Christoph
Published: November 2023

Liebe Kolleginnen und Kollegen,

es freut mich sehr, Ihnen in der aktuellen Ausgabe interessante Beiträge
vorzustellen zu können, die entweder aus neurologischen oder psychiatrischen
Arbeitsgruppen kommen, aber jeweils auch von besonderem Interesse für das
andere Fachgebiet sind.
The French Experience with a Population-Based Esophageal Atresia Registry (RENATO)
Authors: Sfeir Rony , Aumar Madeleine , Sharma Dyuti , Labreuche Julien , Dauchet Luc , Gottrand Frederic
Published: November 2023

This paper presented a national register for esophageal atresia (EA) started in January 2008. We report our experience about the conception of this database and its coordination. Data management and data quality are also detailed. In 2023, more than 2,500 patients with EA are included. Prevalence of EA in France was calculated at 1.8/10,000 live birth. Main clinical results are listed with scientific publications issued directly from the register.
Acylative and Silylative Dynamic Kinetic Resolutions of 6-Hydroxy-2H-pyran-3(6H)-one Catalyzed by Chiral Guanidines
Authors: Nakata Kenya , Miyazaki Kanako
Published: November 2023

Chiral guanidine-catalyzed acylative and silylative dynamic kinetic resolutions of 6-hydroxy-2H-pyran-3(6H)-one were achieved. We succeeded in developing a non-enzymatic acylative dynamic kinetic resolution of 6-hydroxy-2H-pyran-3(6H)-one with high enantioselectivity, which could be improved by recrystallization. In contrast, the non-enzymatic silylative dynamic kinetic resolution of 6-hydroxy-2H-pyran-3(6H)-one was accomplished with a moderate selectivity for the first time.
Anthropometric, Metabolic, and Endocrine Parameters as Predictors of
Estimated Average Glucose and Other Biomarkers of Dysglycemia in Women with
Different Phenotypes of Polycystic Ovary Syndrome
Authors: de Medeiros Sebastião Freitas , Winck Yamamoto de Medeiros Ana Lin , Souto de Medeiros Matheus Antônio , da Silva Carvalho Anna Bethany , Yamamoto Marcia W. , M. Soares José , Baracat Edmund C.
Published: November 2023

The aim of the study was to evaluate the efficacy of anthropometric, metabolic,
and endocrine abnormalities as predictors of estimated average glucose and other
biomarkers of dysglycemia in women with different phenotypes of polycystic ovary
syndrome (PCOS). This cross-sectional study included 648 women with PCOS and 330
controls. A single protocol of investigation was applied for all subjects. PCOS
women were divided by phenotypes according to the Rotterdam criteria. Biomarkers
of dysglycemia were considered dependent variables and anthropometric, lipid,
and hormone alterations as independent variables using univariate and
multivariate logistic regressions. Univariate logistic regression analysis,
controlled for age and BMI, showed that many biomarkers of dysglycemia could be
predicted by anthropometric, lipid, and endocrine variables. Multivariate
logistic models showed that in non-PCOS women estimated average glucose (eAG)
was predicted by lower TSH levels (OR=0.39; p=0.045); fasting
glucose was predicted by increased T (OR=2.3). For PCOS, phenotype A,
eAG was predicted by decreased HDL-C (OR=0.17, p=0.023) and high
levels of free estradiol (OR=7.1, p<0.001). Otherwise, in PCOS,
phenotype D, eAG was predicted by higher levels of HDL-C. The current study
demonstrated that eAG was poorly predicted by anthropometric, lipid, and hormone
parameters. Nevertheless, without adding significant benefits, it was comparable
with other established markers of dysglycemia in women with different PCOS
phenotypes.
Reliability of Anaerobic Contributions during a Single Exhaustive
Knee-extensor Exercise
Authors: Luches-Pereira Gabriel , Kalva-Filho Carlos Augusto , Bertucci Danilo Rodrigues , de Carvalho Carlos Dellavechia , Barbieri Ricardo Augusto , Papoti Marcelo
Published: November 2023

The total anaerobic contribution (AC[La-]+PCr) is a valid and
reliable methodology. However, the active muscle mass plays an important role in
the AC[La-]+PCr determination, which might influence its
reliability. Thus, this study aimed to investigate the effects of two exhaustive
intensities on the reliability of the AC[La-]+PCr during a
one-legged knee extension (1L-KE) exercise. Thirteen physically active males
were submitted to a graded exercise to determine the peak power output (PPO) in
the 1L-KE. Then, two constant-load exercises were conducted to task failure at
100% (TTF100) and 110% (TTF110) of PPO,
and the exercises were repeated on a third day. The blood lactate accumulation
and the oxygen uptake after exercise were used to estimate the anaerobic lactic
and alactic contributions, respectively. Higher values of
AC[La-]+PCr were found after the TTF100
compared to TTF110 (p=0.042). In addition, no significant
differences (p=0.432), low systematic error (80.9 mL), and a
significant ICC (0.71; p=0.004) were found for
AC[La-]+PCr in the TTF100. However, an
elevated coefficient of variation was found (13.7%). In conclusion, we
suggest the use of the exhaustive efforts performed at 100% of the PPO
with the 1L-KE model, but its elevated individual variability must be carefully
considered in future studies.
Social and Health Information Platform: Piloting a Standards-Based, Digital Platform Linking Social Determinants of Health Data into Clinical Workflows for Community-Wide Use
Authors: Khurshid Anjum , Hautala Matti , Oliveira Eliel , Lakshminarayanan Vidya , Abrol Vishal , Collier Joshua , Rosseau Justin , Granado Linda , Nallaparaju Shreya , Mitra Kanishka , Sohail Rania
Published: November 2023

Background Social determinants of health (SDoH)a are increasingly recognized as a main contributor to clinical health outcomes, but the technologies and workflows within clinics make it difficult for health care providers to address SDoH needs during routine clinical visits.

Objectives Our objectives were to pilot a digital platform that matches, links, and visualizes patient-level information and community-level deidentified data from across sectors; establish a technical infrastructure that is scalable, generalizable, and interoperable with new datasets or technologies; employ user-centered codesign principles to refine the platform's visualizations, dashboards, and alerts with community health workers, clinicians, and clinic administrators.

Methods We used privacy-preserving record linkage (PPRL) tools to ensure that all identifiable patient data were encrypted, only matched and displayed with consent, and never accessed or stored by the data intermediary. We used limited data sets (LDS) to share nonidentifiable patient data with the data intermediary through a health information exchange (HIE) to take advantage of existing partner agreements, technical infrastructure, and community clinical data.

Results The platform was successfully piloted in two Federally Qualified Health Clinics by 26 clinic staff. SDoH and demographic data from findhelp were successfully linked, matched, and displayed with clinical and demographic data from the HIE, Connxus. Pilot users tested the platform using real-patient data, guiding the refinement of the social and health information platform's visualizations and alerts. Users emphasized the importance of visuals and alerts that gave quick insights into individual patient SDoH needs, survey responses, and clinic-level trends in SDoH service referrals.

Conclusion This pilot shows the importance of PPRL, LDS, and HIE-based data intermediaries in sharing data across sectors and service providers for scalable patient-level care coordination and community-level insights. Clinic staff are integral in designing, developing, and adopting health technologies that will enhance their ability to address SDoH needs within existing workflows without adding undue burdens or additional stress.