bluuebunny/crossref_metadata_2025 huggingface dataset:
Ações de gerência do cuidado a pacientes com COVID-19 internados em enfermarias: protocolo de revisão de escopo
Authors: Peçanha Sônia Cristina Chagas , Christovam Barbara Pompeu , Souza Cláudio José de
Published: August 2022

Objetivo: apresentar os passos metodológicos de um protocolo de revisão de escopo para mapear as evidências disponíveis acerca das ações de gerência do cuidado a pacientes com COVID-19 internados em enfermarias. Método: trata-se de um protocolo de revisão de escopo desenvolvido conforme a metodologia proposta pelo manual do JBI e Checklist PRISMA-ScR. Utilizou-se a estratégia do acrômio PCC para formular a questão de pesquisa, sendo considerados elegíveis, os estudos que atendam a pergunta “Quais são as ações de gerência do cuidado, realizadas pelo enfermeiro, para o paciente com COVID-19, internado em enfermaria?”, a serem pesquisados nas bases de dados eletrônicas MEDLINE via PubMed, LILACS, Scopus, Embase, Web of Science, Cochrane, CINAHL, BVS e Google Scholar. A seleção dos estudos ocorrerá de forma independente por dois pesquisadores, usando um instrumento construído pelos mesmos no programa Microsoft Office Excel Online para auxiliar na síntese e extração dos dados que serão compilados em figuras e fluxogramas, seguidos de discussão e menção das implicações de ordem prática para a atuação do enfermeiro na gestão do cuidado. Considerações finais: As descobertas poderão subsidiar a assistência a pacientes de outras patologias caracterizadas por síndrome respiratória.
ASSOCIATION BETWEEN PSYCHOLOGICAL UPSET AND IRRITABLE BOWEL SYNDROME AMONG NURSING STUDENTS AT KSA
Authors: SOLİMAN Manal , AHMED QALAWA Shereen
Published: August 2022

Irritable bowel syndrome (IBS) is a fairly common disorder that occurs in the general population. IBS is a functional bowel disorder associated with decreased work productivity. The aim of this study is to investigate the relationship between psychological upset and irritable bowel syndrome among nursing students at the Hafr Albatin University king Saudi Arabia. A descriptive cross-sectional research design on 223 student nurses from Hafr Al Batin university. One tool is divided into three main parts; demographic data, student's psychological upset, and irritable bowel syndrome manifestations sheet. According to the results of the study there is a statistically significant correlation between students' psychological stiffness and manifestations of irritable bowel syndrome (p
Evaluasi Kemampuan Kesuburan Tanah di Kecamatan Tutur Pasuruan
Authors: A.M Arief , W Bakti Wisnu , Midarti Wanti
Published: August 2022

The aim of this study was to discover soil fertility indicators for coffee cultivation in Tutur District as well as the variables that influence soil fertility levels for coffee cultivation land. A survey method was employed in this investigation. The findings of the chemical characteristics test were utilized as a guide for creating maps of the land fertility potential for coffee plants in Tutur Village. These maps included information on water content, soil pH, C-Organic content, N-total content, Phosphorus content, base saturation, and CEC. According to this study, Tutur District's coffee farming may be classified into three soil fertility status categories: low, medium, and high fertility status. The slope of the land is the factor that has the biggest impact on the soil fertility in the Tutur District's coffee growing region; the steeper the slope, the less fertile the soil is.
Pengaruh Aplikasi Perendaman Berbagai Jenis Media Tanam dengan Beberapa Pupuk Cair Terhadap Kualitas dan Tingkat Kesukaan Konsumen Microgreen Wheatgrass (Triticum aestivum L)
Authors: Maulidiyah Indah , Lestari Mahayu Woro , Mardiyani Siti Asmaniyah
Published: August 2022

Wheatgrass microgreen is commonly ingested as juice, which is high in vitamins, minerals, enzymes, and chlorophyll. The purpose of this study was to see how different liquid fertilizers affected the growth and quality, of wheatgrass microgreen when planted in different types of planting media., The Factorial Randomized Block Design was used in this study, with two factors: fertilizer treatment factor (P1 = Eco Enzyme 15ml/1L, P2 =Liquid Organic Fertilizer 10ml/1L, and P3 = AB mix 800ppm, and planting media factor M1 = cocopiet, M2 = rockwool, and M3 = nonwoven fabric. The results showed that the combination of planting media and liquid fertilizer had a significant effect on the amount of chlorophyll in microgreen wheatgrass, the combination of cocopeat and eco-enzyme growing media produced microgreen wheatgrass with the highest chlorophyll content(28,3). The combination of cocopeat and eco-enzyme treatment resulted the cleanest media without any fungal or bacterial contamination. Consumers' level of preference test for juice with AB mix treatment is more favored by consumers. In general, the results of this study indicate that microgreen wheatgrass grown on cocopeat and eco-enzyme media has the best combination of treatments, so it is prospective to be developed as a model of microgreen cultivation technology that is rich in chlorophyll content and is free from fungi and is potential as a raw material for natural healthy drinks rich in nutrients.
Efek Residu Media Tanam dengan Penambahan Kompos Secara Berulang Terhadap Pertumbuhan dan Serapan Hara Nitrogen Tanaman Kailan
Authors: Sholihah Anis , Sugianto Agus
Published: August 2022

This study aims to determine the potential of planting media residues from rice crop residues in the second planting period which were given input of a mixture of kiapu compost and straw with various compositions with the indicator plant used was kailan which belongs to the potential vegetable group and has a fairly high nutritional value. The study used a randomized block design with treatments consisting of 5 compost mixtures and 2 comparison treatments, namely pearl NPK fertilizer treatment and control so the treatment was as follows; M0 : control, M1 : 100% kiapu, M2 : 75% kiapu + 25% rice straw, M3 : 50% kiapu + 50% rice straw, M4 : 25% kiapu + 75% rice straw, M5 : 100% straw and M6 : NPK. The results showed that repeated application of the compost mixture was able to give a positive residual effect so that it could increase growth, yield and N uptake in kailan plants. The increase in plant length due to the addition of repeated compost ranged from 1.96% to 22.79%, leaf area ranged from 11.28% to 75.63% and total plant fresh weight ranged from 28.85% to 59.94%. The treatment with compost significantly increased root N uptake, canopy N uptake and total N uptake compared to control, ranging from 223.67% to 283.36% at root N uptake, 175.03% to 232.47% at canopy N uptake and 194, 43% to 241.63% on the total N uptake of plants.
Balıkçılık Teknolojisi Mezunlarının Memnuniyet ve İstihdam Araştırması: ÇOMÜ GUBY Örneği
Authors: ZORLU Ömer , ERGÜN Sebahattin , AYVAZ Zayde
Published: August 2022

Bu çalışmanın amacı, Balıkçılık Teknolojisi bölümü mezunlarının istihdam, kariyer ve memnuniyet durumlarının anket yardımıyla incelenmesidir. Bunun için Çanakkale Onsekiz Mart Üniversitesi Gökçeada Uygulamalı Bilimler Yüksekokulu Balıkçılık Teknolojisi Bölümü’nden 2014-2018 yıllarında mezun olan öğrencilere; yaş aralıkları, cinsiyetleri, kendi istekleri ile bölümü seçip seçmedikleri, memnuniyet dereceleri, herhangi bir işte çalışıp çalışmadıkları, iş alanlarının mezun oldukları bölümle ilgili olup olmamasındaki düşünceleri ve bölümü tavsiye edip etmediklerine dair sorular bir anket ile yöneltilmiştir. Ankete 92 kişi katılmıştır ve formlar yüz yüze veya sosyal medya (Facebook, Twitter, Instagram ve WhatsApp) araçları ile ulaştırılmıştır. Anket sonuçlarına göre, bölüme isteyerek gelen öğrencilerin oranın %34,8 ve benzer olarak mezun olduğu alanda çalışma istekleri oranının %28,8 olduğu, ancak katılımcıların büyük çoğunluğunun (%71,7) farklı alanlarda da olsa iş bulduğu anlaşılmaktadır. Mezun olduğu bölümden memnun olan katılımcıların oranının (%33,7) ve bunun bir etkisi olarak da bölümü tavsiye eden (%29,3) katılımcı oranının da benzer olduğu görülmektedir.
Çankırı İlinde Uygulanan Akkaraman Islahı Alt Projelerine Dahil Olan Koyun İşletmelerinin Bazı Verim ve Yapısal Özellikleri
Authors: ÜNAL Gökce , ÜNAL İlker , SAKAR Çağrı Melikşah
Published: August 2022

Bu çalışmada 2011 yılından itibaren Çankırı'da yürütülen Akkaraman Irkı Koyun Islahı Alt projelerine dahil olan Akkaraman koyun işletmelerinin bazı verim, sürü yönetimi, ıslah ve yapısal özelliklerinin araştırılması amaçlanmıştır. Araştırmada, “Çankırı İli Akkaraman Irkı Koyun Islahı Alt Projeleri” kapsamında Akkaraman ırkı koyun yetiştiriciliği yapan 57 adet işletmeciile tam sayım yöntemine göre yüz yüze anket yapılmıştır. Elde edilen veriler SPSS paket programı kullanılarak analiz edilmiştir. Analizlerde tanımlayıcı istatistikler ve frekanslar belirlenmiştir. Araştırmada yetiştiricilerin yaş ortalaması 50.3 olarak tespit edilmiştir. Çiftliklerde ortalama anaç koyun sayısı 260 baş’tır. İşletmelerin tamamında yaygın otlatma sistemi uygulanmakta olup hayvanlar yılın 8 ayı merada, 4 ayı ahırda tutulmaktadır. Yetiştiricilerin %79’u mera alanlarını yeterli bulmamaktadır. Koyunların ilk koça verilme yaşı ortalama 18 ay, ortalama üretken ömrü ise 57 ay olarak belirlenmiştir. İşletmelerde tekiz ve ikiz doğum oranları sırasıyla %74 ve %25.5 olarak belirlenmiştir. Kuzuların ortalama sütten kesim yaşı 130 gündür. Yetiştirme döneminde koç başına düşen koyun sayısı 35 olarak belirlenmiştir. Yetiştiricilerin %47’si kuzuları doğumdan sonraki ilk 2 hafta, %53'ü ise 3. ve 4. haftalarda başlangıç yemi ile beslemeye başladıklarını bildirmiştir. Ayrıca işletmelerin %90’ının yem bitkileri ürettiği belirlenmiştir. Sonuç olarak, Çankırı İli Akkaraman Koyun Yetiştiriciliği Alt Projesi kapsamındaki koyun işletmelerinin bazı verim ve sürü yönetim özelliklerine ilişkin mevcut durumu tespit edilmiştir.
Exploitation of an operative wave forecast system for energy resource assessment in the Mediterranean Sea
Authors: Carillo Adriana , Pisacane Giovanna , Struglia Maria Vittoria
Published: August 2022

Ocean Energy is now emerging as a viable long-term form of renewable energy, which might contribute around 10% of EU power demand by 2050, if sufficient support is guaranteed along its road to full commercialization, allowing to further demonstrate the reliability, robustness and overall economic competitiveness of technologies. Although wave energy is still less developed than other marine renewables, its high density, great potential and minimal environmental impact have renewed the interest of developers, investors and governments globally, also in view of the increasing awareness of climate change and of the necessity to reduce carbon emissions. In parallel with technological development, the reliable characterization of wave climate and of the associated energy resource is crucial to the design of efficient Wave Energy Converters and to an effective site-technology matching, especially in low-energy seas. The preliminary scrutiny of suitable technologies and the identification of promising sites for their deployment often rely on wave climatological atlases, yet a more detailed characterization of the local resource is needed to account for high-frequency spatial and temporal variability that significantly impact power generation and the economic viability of WEC farms. We present a high-resolution assessment of the wave energy resource at specific locations in the Mediterranean Sea, based on a 7-years dataset derived from the operative wave forecast system that has been developed at ENEA and has been running since 2013. The selected areas correspond to the target regions of the Blue Deal project, where energy resource estimates were combined with technical and environmental considerations, so as to identify optimal sites for Blue Energy exploitation, from a Maritime Spatial Planning perspective. The available resource at selected sites is analysed together with site theoretical productivity for three state-of-the art WECs, showing interesting potential for future deployment.
Multiscale spatiotemporal dynamics analysis of urban green space: Implications for green space planning in the rapid urbanizing Hefei City, China
Authors: Li Ying-Ying , Ren Bao-Ting , Chen Yong-Sheng , Huang Lei-Chang , Sun Cai-Ge
Published: August 2022

Under the pressure of rapid urbanization, the spatiotemporal dynamics of urban green spaces (UGS) have enormous impacts on the local ecological system and environment at different scales. In this study, UGS in Hefei City, which has experienced rapid urbanization from 1995 to 2015, were extracted based on time-series Landsat-5 TM and Landsat-8 OLI images, and different types of parks were mapped based on GF-2 images combined with multi-source metadata. Dynamic patterns of green space were examined by drawing the spatial variations of green space at the city, inner city, and park scales. Results revealed that: (1) At the city level, UGS decreased with the rapid urbanization, especially farmland sharply lost in areas around existing urban areas and along the transportation corridors. (2) At the inner-city level, concentric analyses showed that UGS changes in different rings had unique trends, and the dynamic changes were the most significant in Rings 2 and 3. Comparative analysis of old and new districts showed that the common characteristics of UGS changes were the transfer-out of farmland and the transfer-in of built-up land, and the newer the region, the more intense the changes. (3) At the park level, the number and area of urban parks were increasing from the center to the periphery, and the type of park gradually changed from single to rich. Significant spatial heterogeneity was identified in the landscape patterns of urban parks based on fishnet cell analysis. It is fundamental to assign urban construction land for socio-economic development, while planning UGS at different scales; moreover, integrating different green space-related policies could protect the UGS and maintain its stability. Only in this way can UGS combine economic, social, and ecological benefits under the background of rapid urbanization.
Locating a novel autosomal recessive genetic variant in the cattle glucokinase gene using only WGS data from three cases and six carriers
Authors: Pollott Geoffrey E. , Piercy Richard J. , Massey Claire , Salavati Mazdak , Cheng Zhangrui , Wathes D. Claire
Published: August 2022

New Mendelian genetic conditions, which adversely affect livestock, arise all the time. To manage them effectively, some methods need to be devised that are quick and accurate. Until recently, finding the causal genomic site of a new autosomal recessive genetic disease has required a two-stage approach using single-nucleotide polymorphism (SNP) chip genotyping to locate the region containing the new variant. This region is then explored using fine-mapping methods to locate the actual site of the new variant. This study explores bioinformatic methods that can be used to identify the causative variants of recessive genetic disorders with full penetrance with just nine whole genome-sequenced animals to simplify and expedite the process to a one-step procedure. Using whole genome sequencing of only three cases and six carriers, the site of a novel variant causing perinatal mortality in Irish moiled calves was located. Four methods were used to interrogate the variant call format (VCF) data file of these nine animals, they are genotype criteria (GCR), autozygosity-by-difference (ABD), variant prediction scoring, and registered SNP information. From more than nine million variants in the VCF file, only one site was identified by all four methods (Chr4: g.77173487A>T (ARS-UCD1.2 (GCF_002263795.1)). This site was a splice acceptor variant located in the glucokinase gene (GCK). It was verified on an independent sample of animals from the breed using genotyping by polymerase chain reaction at the candidate site and autozygosity-by-difference using SNP-chips. Both methods confirmed the candidate site. Investigation of the GCR method found that sites meeting the GCR were not evenly spread across the genome but concentrated in regions of long runs of homozygosity. Locating GCR sites was best performed using two carriers to every case, and the carriers should be distantly related to the cases, within the breed concerned. Fewer than 20 animals need to be sequenced when using the GCR and ABD methods together. The genomic site of novel autosomal recessive Mendelian genetic diseases can be located using fewer than 20 animals combined with two bioinformatic methods, autozygosity-by-difference, and genotype criteria. In many instances it may also be confirmed with variant prediction scoring. This should speed-up and simplify the management of new genetic diseases to a single-step process.
Organization of the mitochondrial genome of Ramulus irregulatiter dentatus (Phasmatidae: Phasmatidae)
Authors: Zhang Congfen , Guo Xiaoqiang
Published: August 2022

Recently, the species of the insect order Phasmatodea, have attracted the interest of more and more enthusiasts. Here, we obtained the complete mitochondrial genome of Ramulus irregulatiter dentatus (R. irregulatiter dentatus), which belongs to the subfamily of Phasmatidae, detected by Illumina next-generation sequencing. The entire mitochondrial genome is 16,060 bp in length and contains a standard set of 13 protein-coding genes, 22 transfer RNA genes (tRNAs), 2 ribosomal RNA genes (rRNAs), and a putative A + T-rich region. The base composition and codon usage were typical of Phasmatodea species. The mitochondrial gene organization (37 genes) was consistent with that of other Phasmatidae. A phylogenetic tree was built from the sequence information of the 13 protein-coding genes by Bayesian analyses. The newly sequenced R. irregulatiter dentatus was most closely related to the family Phasmatidae. The complete mitochondrial genome of R. irregulatiter dentatus also provides valuable molecular information for future studies on Phasmatidae insect taxonomy and a framework to unveil more of their cryptic and unknown diversity, so that it can be used to control forest pests and protect crops.
Case report and analysis: Behçet’s disease with lower extremity vein thrombosis and pseudoaneurysm
Authors: Wang Han-Lu , Zhang Jian-Hui , Wu Yi-Cheng , Lin Jia-Li , Tang Yi , Liao Li-Sheng , Luo Jie-Wei , Yu Qing-Hua , Fang Zhu-Ting
Published: August 2022

Background

Behçet’s disease (BD) is a unique autoimmune chronic systemic vasculitis that affects veins and arteries of all sizes. BD can lead to recurrent vascular events, especially venous thrombosis, with an incidence rate of 40%, or pseudoaneurysms formed under long-term inflammatory reaction or iatrogenic stimulation. BD-related risk factors promote endothelial dysfunction, platelet activation and overactivation of tissue factors leading to mural inflammatory thrombi. Thrombosis may be the first clinical manifestation of BD.

Case presentation

A 32-year-old man complaining of progressive swelling and pain in the right lower extremity for 30 days was initially diagnosed with “venous thrombosis of the right lower extremity,” using color Doppler ultrasonography. Patient underwent inferior vena cava filter placement combined with deep vein angioplasty of the right lower extremity and catheter-directed urokinase thrombolysis. Postoperative oral anticoagulant therapy was administered. However, the patient was readmitted 20 days later for pulsatile pain in the right groin. Prior medical history included 4 years of repeated oral and perineal ulcers, and 2 months of blurred vision. Abdominal computed tomography angiography (CTA) revealed rupture of the right common iliac artery (CIA) and left internal iliac artery (IIA), complicated by a pseudoaneurysm. Based on the clinical manifestations and other auxiliary examination results, the patient was re-diagnosed with “BD combined with deep venous thrombosis of the right lower extremity and an iliac artery pseudoaneurysm.” Stent implantation was performed for iliac artery pseudoaneurysm after symptoms were controlled with timely immunosuppressive therapy. After endovascular treatment, the patient underwent continued immunosuppressive therapy and dynamic reexaminations of abdominal CTA, which revealed that a small amount of contrast agent at the stent in the right CIA continued to flow into the cavity of the pseudoaneurysm; in addition, the size of the pseudoaneurysm was gradually increasing. Therefore, the patient underwent a second stent implantation for iliac artery pseudoaneurysm, and the condition improved further.

Conclusion

The importance of early diagnosis of BD should be recognized, and the choice of interventional and surgical procedures should be carefully evaluated, as this may trigger further damage to vascular access in BD patients with aneurysm.
Exploring the diversity and structural response of sediment-associated microbiota communities to environmental pollution at the siangshan wetland in Taiwan using environmental DNA metagenomic approach
Authors: Salah-Tantawy Ahmed , Chang Ching-Sung Gavin , Liu Min-Yun , Young Shuh-Sen
Published: August 2022

One of the most crucial struggles for the aquatic ecosystem and modern society is environmental pollution. New approaches, such as the environmental DNA Metagenomic approach, have become a powerful tool to investigate the abundance and diversity of sediment-associated microbiota communities. Nevertheless, information on the response of microbial populations to heavy metals (HMs) in the coastal sediments of Taiwan is scarce. 44 sediment samples were collected from the Siangshan wetland in Taiwan (Taiwan Strait) at two different depths (surface and sub-surface) to measure various environmental variables viz., heavy metals, total organic matters, and sediment composition. Moreover, the abundance and structure of sediment-associated microbiota were examined using high-throughput 16S rRNA gene (V3-V4) next-generation sequencing, to explore the response of the microbiota community to environmental variables. According to the computed pollution indices viz., sediment quality guidelines (SQGs), enrichment factor (EF), geo-accumulation index (Igeo), pollution index (PI), and pollution load index (PLI), the studied sites were classified as unpolluted, moderately polluted, and extremely polluted. Our results revealed that the majority of high-quality reads were assigned to bacteria (~ 95.05%), Archaea (~ 4.83%), and 0.13% were unclassified. Study sites were dominated largely by Proteobacteria with a total of 38.02% across all sediment samples, followed by Bacteroidota (7.18%), Desulfobacterota (6.97%), Actinobacterota (6.68%), Cyanobacteria (5.84%), Chloroflexota (4.18%), Planctomycetota (4.16%), and Firmicutes (3.69%). Beta diversity (Non-metric dimensional scaling analysis, nMDS) explained that surface and sub-surface groups had significantly different microbial community compositions (p = 0.01). Meanwhile, polluted sites exhibited more richness and diversity than unpolluted sites. Redundancy analysis (RDA) illustrated that Mn, Cu, Al, Co, Ni, Sand, and Cr had a sizable effect on the structure of microbiota communities (at the class level). This work highlights the potential responsibility of environmental variables in shaping the sedimentary microbiota of the Siangshan wetland via integrating various ecological variables with alteration of the microbiota composition.
The role of bacterial cyclic di-adenosine monophosphate in the host immune response
Authors: Cheng Xingqun , Ning Jia , Xu Xin , Zhou Xuedong
Published: August 2022

Cyclic di-adenosine monophosphate (c-di-AMP) is a second messenger which is widely used in signal transduction in bacteria and archaea. c-di-AMP plays an important role in the regulation of bacterial physiological activities, such as the cell cycle, cell wall stability, environmental stress response, and biofilm formation. Moreover, c-di-AMP produced by pathogens can be recognized by host cells for the activation of innate immune responses. It can induce type I interferon (IFN) response in a stimulator of interferon genes (STING)-dependent manner, activate the nuclear factor kappa B (NF-κB) pathway, inflammasome, and host autophagy, and promote the production and secretion of cytokines. In addition, c-di-AMP is capable of triggering a host mucosal immune response as a mucosal adjuvant. Therefore, c-di-AMP is now considered to be a new pathogen-associated molecular pattern in host immunity and has become a promising target in bacterial/viral vaccine and drug research. In this review, we discussed the crosstalk between bacteria and host immunity mediated by c-di-AMP and addressed the role of c-di-AMP as a mucosal adjuvant in boosting evoked immune responses of subunit vaccines. The potential application of c-di-AMP in immunomodulation and immunotherapy was also discussed in this review.
Atypical acute disseminated encephalomyelitis with systemic inflammation after a first dose of AztraZaneca COVID-19 vaccine. A case report
Authors: Bastide Laure , Perrotta Gaetano , Lolli Valentina , Mathey Céline , Vierasu Ortensa-Irina , Goldman Serge , Vandergheynst Frédéric
Published: August 2022

Background

Only a few cases of acute disseminated encephalomyelitis (ADEM) following coronavirus disease 19 (COVID-19) vaccination have been described since the beginning of the vaccination campaign.

Results

Here we report the first case of central nervous system (CNS) demyelination with systemic inflammatory findings on whole body 19-fluorodeoxyglucose positron emission tomography with computed tomography (FDG-PET/CT) following the ChAdOx1 nCoV-19 vaccine.

Conclusions

Clinicians should stay aware of potential new adverse events after immunization.
Shared nociceptive dorsal root ganglion neurons participating in acupoint sensitization
Authors: Li Wanrong , Liu Jia , Chen Aiwen , Dai Danqing , Zhao Tiantian , Liu Qiong , Song Jianren , Xiong Lize , Gao Xiao-Fei
Published: August 2022

When the body is under pathological stress (injury or disease), the status of associated acupoints changes, including decreased pain threshold. Such changes in acupoint from a “silent” to an “active” state are considered “acupoint sensitization,” which has become an important indicator of acupoint selection. However, the mechanism of acupoint sensitization remains unclear. In this study, by retrograde tracing, morphological, chemogenetic, and behavioral methods, we found there are some dorsal root ganglion (DRG) neurons innervating the ST36 acupoint and ipsilateral hind paw (IHP) plantar simultaneously. Inhibition of these shared neurons induced analgesia in the complete Freund’s adjuvant (CFA) pain model and obstruction of nociceptive sensation in normal mice, and elevated the mechanical pain threshold (MPT) of ST36 acupoint in the CFA model. Excitation of shared neurons induced pain and declined the MPT of ST36 acupoint. Furthermore, most of the shared DRG neurons express TRPV1, a marker of nociceptive neurons. These results indicate that the shared nociceptive DRG neurons participate in ST36 acupoint sensitization in CFA-induced chronic pain. This raised a neural mechanism of acupoint sensitization at the level of primary sensory transmission.
Tumor acidity: From hallmark of cancer to target of treatment
Authors: Bogdanov Alexey , Bogdanov Andrey , Chubenko Viacheslav , Volkov Nikita , Moiseenko Fedor , Moiseyenko Vladimir
Published: August 2022

Tumor acidity is one of the cancer hallmarks and is associated with metabolic reprogramming and the use of glycolysis, which results in a high intracellular lactic acid concentration. Cancer cells avoid acid stress major by the activation and expression of proton and lactate transporters and exchangers and have an inverted pH gradient (extracellular and intracellular pHs are acid and alkaline, respectively). The shift in the tumor acid–base balance promotes proliferation, apoptosis avoidance, invasiveness, metastatic potential, aggressiveness, immune evasion, and treatment resistance. For example, weak-base chemotherapeutic agents may have a substantially reduced cellular uptake capacity due to “ion trapping”. Lactic acid negatively affects the functions of activated effector T cells, stimulates regulatory T cells, and promotes them to express programmed cell death receptor 1. On the other hand, the inversion of pH gradient could be a cancer weakness that will allow the development of new promising therapies, such as tumor-targeted pH-sensitive antibodies and pH-responsible nanoparticle conjugates with anticancer drugs. The regulation of tumor pH levels by pharmacological inhibition of pH-responsible proteins (monocarboxylate transporters, H+-ATPase, etc.) and lactate dehydrogenase A is also a promising anticancer strategy. Another idea is the oral or parenteral use of buffer systems, such as sodium bicarbonate, to neutralize tumor acidity. Buffering therapy does not counteract standard treatment methods and can be used in combination to increase effectiveness. However, the mechanisms of the anticancer effect of buffering therapy are still unclear, and more research is needed. We have attempted to summarize the basic knowledge about tumor acidity.
Intelligence, emotional intelligence, and emo-sensory intelligence: Which one is a better predictor of university students’ academic success?
Authors: Pishghadam Reza , Faribi Maryam , Kolahi Ahari Mahtab , Shadloo Farzaneh , Gholami Mohammad Javad , Shayesteh Shaghayegh
Published: August 2022

The primary aim of this study was to determine the role of psychometric intelligence (IQ), emotional intelligence (EQ), and emo-sensory intelligence (ESQ) in university students’ academic achievement. To this end, 212 university students at different academic levels, composed of 154 females and 58 males, were asked to complete the Raven’s Progressive Matrices, the Bar-On Emotional Quotient Inventory, and the Emo-Sensory Intelligence Scale. Data were then matched with students’ Grade Point Averages as a measure of their academic achievement. The results revealed that students’ level of IQ and EQ could positively predict their academic achievement. In the case of their ESQ level, its auditory sub-component was found to be a positive predictor of academic success. Results were discussed, and possible implications and applications for increasing students’ chances for success were presented.
Correlation Analysis of Factors Affecting Firm Performance and Employees Wellbeing: Application of Advanced Machine Learning Analysis
Authors: Pap Jozsef , Mako Csaba , Illessy Miklos , Dedaj Zef , Ardabili Sina , Torok Bernat , Mosavi Amir
Published: August 2022

Given the importance of identifying key performance points in organizations, this research intends to determine the most critical intra- and extra-organizational elements in assessing the performance of firms using the European Company Survey (ECS) 2019 framework. The ECS 2019 survey data were used to train an artificial neural network optimized using an imperialist competitive algorithm (ANN-ICA) to forecast business performance and employee wellbeing. In order to assess the correctness of the model, root mean square error (RMSE), mean absolute percentage error (MAPE), mean square error (MSE), correlation coefficient (r), and determination coefficient (R2) have been employed. The mean values of the performance criteria for the impact of internal and external factors on firm performance were 1.06, 0.002, 0.041, 0.9, and 0.83, and the value of the performance metrics for the impact of internal and external factors on employee wellbeing were 0.84, 0.0019, 0.0319, 0.83, and 0.71 (respectively, for MAPE, MSE, RMSE, r, and R2). The great performance of the ANN-ICA model is indicated by low values of MAPE, MSE, and RMSE, as well as high values of r and R2. The outcomes showed that “skills requirements and skill matching” and “employee voice” are the two factors that matter most in enhancing firm performance and wellbeing.
Effect of D1- and D2-like Dopamine Receptor Antagonists on the Rewarding and Anxiolytic Effects of Neurotensin in the Ventral Pallidum
Authors: Ollmann Tamás , Lénárd László , Péczely László , Berta Beáta , Kertes Erika , Zagorácz Olga , Hormay Edina , László Kristóf , Szabó Ádám , Gálosi Rita , Karádi Zoltán , Kállai Veronika
Published: August 2022

Background: Neurotensin (NT) acts as a neurotransmitter and neuromodulator in the central nervous system. It was shown previously that NT in the ventral pallidum (VP) has rewarding and anxiolytic effects. NT exerts its effect in interaction with dopamine (DA) receptors in numerous brain areas; however, this has not yet been investigated in the VP. The aim of this study was to examine whether the inhibition of D1-like and D2-like DA receptors of the VP can modify the above mentioned effects of NT. Methods: Microinjection cannulas were implanted by means of stereotaxic operations into the VP of male Wistar rats. The rewarding effect of NT was examined by means of a conditioned place preference test. Anxiety was investigated with an elevated plus maze test. To investigate the possible interaction, D1-like DA receptor antagonist SCH23390 or D2-like DA receptor antagonist sulpiride were microinjected prior to NT. All of the drugs were also injected independently to analyze their effects alone. Results: In the present experiments, both the rewarding and anxiolytic effects of NT in the VP were prevented by both D1-like and D2-like DA receptor antagonists. Administered on their own, the antagonists did not influence reward and anxiety. Conclusion: Our present results show that the activity of the D1-like and D2-like DA receptors of the VP is a necessary requirement for both the rewarding and anxiolytic effects of NT.
Recent Trends in Nanomedicine-Based Strategies to Overcome Multidrug Resistance in Tumors
Authors: Khan Muhammad Muzamil , Torchilin Vladimir P.
Published: August 2022

Cancer is the leading cause of economic and health burden worldwide. The commonly used approaches for the treatment of cancer are chemotherapy, radiotherapy, and surgery. Chemotherapy frequently results in undesirable side effects, and cancer cells may develop resistance. Combating drug resistance is a challenging task in cancer treatment. Drug resistance may be intrinsic or acquired and can be due to genetic factors, growth factors, the increased efflux of drugs, DNA repair, and the metabolism of xenobiotics. The strategies used to combat drug resistance include the nanomedicine-based targeted delivery of drugs and genes using different nanocarriers such as gold nanoparticles, peptide-modified nanoparticles, as well as biomimetic and responsive nanoparticles that help to deliver payload at targeted tumor sites and overcome resistance. Gene therapy in combination with chemotherapy aids in this respect. siRNA and miRNA alone or in combination with chemotherapy improve therapeutic response in tumor cells. Some natural substances, such as curcumin, quercetin, tocotrienol, parthenolide, naringin, and cyclosporin-A are also helpful in combating the drug resistance of cancer cells. This manuscript summarizes the mechanism of drug resistance and nanoparticle-based strategies used to combat it.
Self-Supervised Adversarial Learning with a Limited Dataset for Electronic Cleansing in Computed Tomographic Colonography: A Preliminary Feasibility Study
Authors: Tachibana Rie , Näppi Janne J. , Hironaka Toru , Yoshida Hiroyuki
Published: August 2022

Existing electronic cleansing (EC) methods for computed tomographic colonography (CTC) are generally based on image segmentation, which limits their accuracy to that of the underlying voxels. Because of the limitations of the available CTC datasets for training, traditional deep learning is of limited use in EC. The purpose of this study was to evaluate the technical feasibility of using a novel self-supervised adversarial learning scheme to perform EC with a limited training dataset with subvoxel accuracy. A three-dimensional (3D) generative adversarial network (3D GAN) was pre-trained to perform EC on CTC datasets of an anthropomorphic phantom. The 3D GAN was then fine-tuned to each input case by use of the self-supervised scheme. The architecture of the 3D GAN was optimized by use of a phantom study. The visually perceived quality of the virtual cleansing by the resulting 3D GAN compared favorably to that of commercial EC software on the virtual 3D fly-through examinations of 18 clinical CTC cases. Thus, the proposed self-supervised 3D GAN, which can be trained to perform EC on a small dataset without image annotations with subvoxel accuracy, is a potentially effective approach for addressing the remaining technical problems of EC in CTC.
Advances in Geriatric Oncology: Exploring Practical Ways to Optimize Treatment in Older Patients with Cancer
Authors: Paillaud Elena , Hamaker Marije E. , Soubeyran Pierre
Published: August 2022

Cancer is a disease associated with aging, with patients over 70 accounting for 50% of newly diagnosed malignancies and 70% of all cancer deaths [...]
Genetics and Epigenetics in Neoplasms with Plasmacytoid Dendritic Cells
Authors: Renosi Florian , Callanan Mary , Lefebvre Christine
Published: August 2022

Plasmacytoid Dendritic Cells (pDC) are type I interferon (IFN)-producing cells that play a key role in immune responses. Two major types of neoplastic counterparts for pDC are now discriminated: Blastic pDC Neoplasm (BPDCN) and Mature pDC Proliferation (MPDCP), associated with myeloid neoplasm. Two types of MPDCP are now better described: Chronic MyeloMonocytic Leukemia with pDC expansion (pDC-CMML) and Acute Myeloid Leukemia with pDC expansion (pDC-AML). Differential diagnosis between pDC-AML and BPDCN is particularly challenging, and genomic features can help for diagnosis. Here, we systematically review the cytogenetic, molecular, and transcriptional characteristics of BPDCN and pDC-AML. BPDCN are characterized by frequent complex karyotypes with recurrent MYB/MYC rearrangements as well as recurrent deletions involving ETV6, IKZF1, RB1, and TP53 loci. Epigenetic and splicing pathways are also particularly mutated, while original processes are dysregulated, such as NF-kB, TCF4, BCL2, and IFN pathways; neutrophil-specific receptors; and cholinergic signaling. In contrast, cytogenetic abnormalities are limited in pDC-AML and are quite similar to other AML. Interestingly, RUNX1 is the most frequently mutated gene (70% of cases). These typical genomic features are of potential interest for diagnosis, and also from a prognostic or therapeutic perspective.
Digital and Interactive Health Interventions Minimize the Physical and Psychological Impact of Breast Cancer, Increasing Women’s Quality of Life: A Systematic Review and Meta-Analysis
Authors: Obrero-Gaitán Esteban , Cortés-Pérez Irene , Calet-Fernández Tania , García-López Héctor , López Ruiz María del Carmen , Osuna-Pérez María Catalina
Published: August 2022

Digital and interactive health interventions (DIHIs), such as virtual-reality-based therapy (VRBT) and smartphone-app-based therapy (SABT), may be useful for reducing the impact of the signs and symptoms of breast cancer (BC) in women. The aim of this meta-analysis was to explore the effect of DIHIs on improving pain, anxiety, depression, quality of life (QoL), and upper extremity (UE) disability-related lymphedema in women with BC. Methods: We searched PubMed Medline, Web of Science, Scopus, CINAHL, Physiotherapy Evidence Database, and SciELO for the period ending February 2022. We included studies that assessed the effect of DIHIs on UE motor disability, pain, anxiety, depression, and QoL in women with BC. The effect size was calculated using Cohen’s standardized mean difference (SMD) and its 95% confidence interval (95% CI). Results: Twenty studies providing data from 1613 women with BC were included. With respect to UE disability, DIHIs increased flexion (SMD, 1.92; 95%CI: −1.16, 2.68), abduction (SMD, 1.66; 95%CI: 0.91, 2.42), external rotation shoulder range of motion (SMD, 1.1; 95%CI: 0.36, 1.85), UE function (SMD, −0.72; 95%CI: −1.31, −0.13), and handgrip strength (SMD, 0.4; 95%CI: 0.21, 0.59). DIHIs reduced pain (SMD, −0.8; 95%CI: −1.31, −0.26), anxiety (SMD, −1.02; 95%CI: −1.71, −0.34), and depression (SMD, −1.57; 95%CI: −3.1, −0.08). Finally, DIHIs increased overall health (SMD, 0.6; 95%CI: 0.31, 0.89). Conclusions: Right at the end of therapy, DIHIs are effective at improving UE function, pain, anxiety, depression, and QoL in women with BC. VRBT has a greater effect than SABT for the assessed outcomes.
Use of Endoscopic Scraper and Cell Block Technique as a Replacement for Conventional Brush for Diagnosing Malignant Biliary Strictures
Authors: Kato Akihisa , Kato Hiroyuki , Naitoh Itaru , Hayashi Kazuki , Yoshida Michihiro , Hori Yasuki , Kachi Kenta , Asano Go , Sahashi Hidenori , Toyohara Tadashi , Kuno Kayoko , Kito Yusuke , Takahashi Satoru , Kataoka Hiromi
Published: August 2022

Histological evidence is essential for diagnosing malignant biliary strictures. However, conventional brush cytology remains the primary method used worldwide, despite its low diagnostic sensitivity and accuracy, as it is technically easy, rapid, and cost-effective. The aim of this study was to evaluate the diagnostic performance of a recently introduced endoscopic scraper, the simplicity of which is comparable to that of a conventional brush, by comparing diagnostic yields and the number of collected cells. The sensitivity of the endoscopic scraper when using the cell block technique was significantly higher than when using brush cytology or a brush with the cell block technique (53.6% vs. 30.9%, p < 0.001; 53.6% vs. 31.6%, p = 0.024, respectively). Quantitative digital image analysis of cell block sections revealed that the median number of cells obtained with the endoscopic scraper was significantly higher than when using the brush (1917 vs. 1014 cells, p = 0.042). Furthermore, seven cases (8.3%; 7/84) were diagnosed by immunohistochemical analysis of the cell block section obtained from the endoscopic scraper. Given its simplicity and greater capacity for sample acquisition, use of the endoscopic scraper in conjunction with the cell block technique could replace brush cytology for the histological diagnosis of malignant biliary strictures.
Homologous Recombination Deficiency Scar: Mutations and Beyond—Implications for Precision Oncology
Authors: van der Wiel Alexander M. A. , Schuitmaker Lesley , Cong Ying , Theys Jan , Van Hoeck Arne , Vens Conchita , Lambin Philippe , Yaromina Ala , Dubois Ludwig J.
Published: August 2022

Homologous recombination deficiency (HRD) is a prevalent in approximately 17% of tumors and is associated with enhanced sensitivity to anticancer therapies inducing double-strand DNA breaks. Accurate detection of HRD would therefore allow improved patient selection and outcome of conventional and targeted anticancer therapies. However, current clinical assessment of HRD mainly relies on determining germline BRCA1/2 mutational status and is insufficient for adequate patient stratification as mechanisms of HRD occurrence extend beyond functional BRCA1/2 loss. HRD, regardless of BRCA1/2 status, is associated with specific forms of genomic and mutational signatures termed HRD scar. Detection of this HRD scar might therefore be a more reliable biomarker for HRD. This review discusses and compares different methods of assessing HRD and HRD scar, their advances into the clinic, and their potential implications for precision oncology.
As Easy as 1, 2, 3? How to Determine CBCT Frequency in Adjuvant Breast Radiotherapy
Authors: Junker Yannis , Düsberg Mathias , Asadpour Rebecca , Klusen Sophie , Münch Stefan , Bernhardt Denise , Combs Stephanie Elisabeth , Borm Kai Joachim
Published: August 2022

The current study aims to assess the suitability of setup errors during the first three treatment fractions to determine cone-beam computed tomography (CBCT) frequency in adjuvant breast radiotherapy. For this, 45 breast cancer patients receiving non-hypofractionated radiotherapy after lumpectomy, including a simultaneous integrated boost (SIB) to the tumor bed and daily CBCT imaging, were retrospectively selected. In a first step, mean and maximum setup errors on treatment days 1–3 were correlated with the mean setup errors during subsequent treatment days. In a second step, dose distribution was estimated using a dose accumulation workflow based on deformable image registration, and setup errors on treatment days 1–3 were correlated with dose deviations in the clinical target volumes (CTV) and organs at risk (OAR). No significant correlation was found between mean and maximum setup errors on treatment days 1–3 and mean setup errors during subsequent treatment days. In addition, mean and maximum setup errors on treatment days 1–3 correlated poorly with dose coverage of the CTVs and dose to the OARs. Thus, CBCT frequency in adjuvant breast radiotherapy should not be determined solely based on the magnitude of setup errors during the first three treatment fractions.
An Integrative Clinical Model for the Prediction of Pathological Complete Response in Patients with Operable Stage II and Stage III Triple-Negative Breast Cancer Receiving Neoadjuvant Chemotherapy
Authors: Chung Wai-Shan , Chen Shin-Cheh , Ko Tai-Ming , Lin Yung-Chang , Lin Sheng-Hsuan , Lo Yung-Feng , Tseng Shu-Chi , Yu Chi-Chang
Published: August 2022

Triple-negative breast cancer (TNBC) is treated with neoadjuvant chemotherapy (NAC). The response to NAC, particularly the probability of a complete pathological response (pCR), guides the surgical approach and adjuvant therapy. We developed a prediction model using a nomogram integrating blood tests and pre-treatment ultrasound findings for predicting pCR in patients with stage II or III operable TNBC receiving NAC. Clinical data before and after the first cycle of NAC collected from patients between 2012 and 2019 were analyzed using univariate and multivariate analyses to identify correlations with pCR. The coefficients of the significant parameters were calculated using logistic regression, and a nomogram was developed based on the logistic model to predict the probability of pCR. Eighty-eight patients were included. Five parameters correlated with the probability of pCR, including the neutrophil-to-lymphocyte ratio, platelet-to-lymphocyte (PLR) ratio, percentage change in PLR, presence of echogenic halo, and tumor height-to-width ratio. The discrimination performance of the nomogram was indicated by an area under the curve of 87.7%, and internal validation showed that the chi-square value of the Hosmer–Lemeshow test was 7.67 (p = 0.363). Thus, the integrative prediction model using clinical data can predict the probability of pCR in patients with TNBC receiving NAC.
Pollen Morphology of Rosa sericea Complex and Their Taxonomic Contribution
Authors: Ullah Fazal , Gao Yun-Dong , Zaman Wajid , Gao Xin-Fen
Published: August 2022

In this research, the pollen morphology of the Rosa sericea complex has been studied by Scanning Electron Microscope (SEM). The present study aimed to provide baseline information for the taxonomic identification of the R. sericea complex taxa. The pollen sculpture, outline, and aperture show variability in the studied taxa. The R. sericea complex includes taxonomically challenging species with undistinguishable boundaries. Identifying taxon boundaries in closely related species is fundamental to investigating evolution and biodiversity. Taxa circumscription of R. sericea complex is insufficient, unresolved, and is a prerequisite for comprehensive systematic revisions. To resolve these issues, we have attempted to identify some important diagnostic characters for the species of R. sericea complex. This research studied different qualitative and quantitative characteristics of R. sericea complex pollen. The qualitative characteristics included polar and equatorial view, sculpture, pollen outline, apertures, exine, size category, and polarity of pollen. In contrast, the quantitative features include polar and equatorial diameter, P/E, colpi length and width, and distance between colpi. Pollen of this complex is mono and tricolporate. The morphological characters studied here provide very few taxonomic details with which to separate the species of the complex into distinct taxa. Some of the studied characters offer important information for species delimitation including sculpture, pollen outline, and apertures.