bluuebunny/crossref_metadata_2025 huggingface dataset:
Bone Marrow Stromal Cell Antigen 2: Is a Potential Neuroinflammation Biomarker of SOD1G93A Mouse Model of Amyotrophic Lateral Sclerosis in Pre-symptomatic Stage
Authors: Xu Xiaojiao , Zhang Jingjing , Li Song , Al-Nusaif Murad , Zhou Qinming , Chen Sheng , Le Weidong
Published: February 2022

Neuroinflammation has long been thought to be associated with amyotrophic lateral sclerosis (ALS) development and progression. However, the exact molecular mechanisms of neuroinflammation underlying ALS remain largely unknown. In the present study, we attempted to elucidate the genetic basis of neuroinflammation in ALS by comparing the transcriptomic profile of the anterior horns of the lumbar spinal cord (AHLSC) between SOD1G93A mice and their wild-type (WT) littermates. Our results revealed that immune-related genes were selectively up-regulated in the AHLSC of pre-symptomatic ALS mice (40 days of age) compared to age-matched WT control mice. Notably, the differential expression level of these immune-related genes became more significant at the symptomatic stage of disease (90 days of age) in the ALS mice. Subsequently, eight genes involved in innate immune response in the AHLSC of ALS mice were further validated by qRT-PCR analysis. Of these genes, bone marrow stromal cell antigen 2 (BST2) was found for the first time to be significantly higher in the AHLSC of pre-symptomatic ALS mice when compared with WT mice. The increasing trend of BST2 expression became more obvious in the symptomatic stage. Immunofluorescent staining further confirmed that BST2 is mainly expressed on microglia in the AHLSC of ALS mice. These findings support the view that immune-related neuroinflammation is involved in the early pathogenesis of ALS, and BST2 may serve as a potential target for ameliorating microglia-mediated neuroinflammation pathologies in ALS.
Dissociation Between Incubation of Cocaine Craving and Anxiety-Related Behaviors After Continuous and Intermittent Access Self-Administration
Authors: Nicolas Celine , Russell Trinity I. , Shaham Yavin , Ikemoto Satoshi
Published: February 2022

Studies using either continuous or intermittent access cocaine self-administration procedures showed that cocaine seeking increases during abstinence (incubation of cocaine craving), and that this effect is higher after intermittent cocaine access. Other studies showed that cocaine abstinence is characterized by the emergence of stress- and anxiety-related states which were hypothesized to increase relapse vulnerability. We examined whether incubation of cocaine craving and anxiety-related behaviors are correlated and whether intermittent cocaine self-administration would potentiate these behaviors during abstinence. Male rats self-administered cocaine either continuously (6 h/day) or intermittently (5 min ON, 25 min OFF × 12) for 14 days, followed by relapse tests after 1 or 21 abstinence days. A group of rats that self-administered saline served as a control. Anxiety-related behaviors were measured on the same abstinence days, using the novelty induced-hypophagia test. Finally, motivation for cocaine was measured using a progressive ratio reinforcement schedule. Lever-presses after 21 abstinence days were higher than after 1 day and this incubation effect was higher in the intermittent access group. Progressive ratio responding was also higher after intermittent cocaine access. Intermittent and continuous cocaine access did not induce anxiety-like responses in the novelty-induced hypophagia test after 1 or 21 abstinence days. Independent of the access condition, incubation of cocaine seeking was not correlated with the novelty-induced hypophagia measures. Results suggest that cocaine-induced anxiety-related states during protracted abstinence do not contribute to incubation of cocaine craving. However, this conclusion is tentative because we used a single anxiety-related measure and did not test female rats.
Temporal Profile of Descending Cortical Modulation of Spinal Excitability: Group and Individual-Specific Effects
Authors: Xu Jiang , Lopez Alejandro J. , Hoque Maruf M. , Borich Michael R. , Kesar Trisha M.
Published: February 2022

Sensorimotor control is modulated through complex interactions between descending corticomotor pathways and ascending sensory inputs. Pairing sub-threshold transcranial magnetic stimulation (TMS) with peripheral nerve stimulation (PNS) modulates the Hoffmann’s reflex (H-reflex), providing a neurophysiologic probe into the influence of descending cortical drive on spinal segmental circuits. However, individual variability in the timing and magnitude of H-reflex modulation is poorly understood. Here, we varied the inter-stimulus interval (ISI) between TMS and PNS to systematically manipulate the relative timing of convergence of descending TMS-induced volleys with respect to ascending PNS-induced afferent volleys in the spinal cord to: (1) characterize effective connectivity between the primary motor cortex (M1) and spinal circuits, mediated by both direct, fastest-conducting, and indirect, slower-conducting descending pathways; and (2) compare the effect of individual-specific vs. standard ISIs. Unconditioned and TMS-conditioned H-reflexes (24 different ISIs ranging from −6 to 12 ms) were recorded from the soleus muscle in 10 able-bodied individuals. The magnitude of H-reflex modulation at individualized ISIs (earliest facilitation delay or EFD and individual-specific peak facilitation) was compared with standard ISIs. Our results revealed a significant effect of ISI on H-reflex modulation. ISIs eliciting earliest-onset facilitation (EFD 0 ms) ranged from −3 to −5 ms across individuals. No difference in the magnitude of facilitation was observed at EFD 0 ms vs. a standardized short-interval ISI of −1.5 ms. Peak facilitation occurred at longer ISIs, ranging from +3 to +11 ms. The magnitude of H-reflex facilitation derived using an individual-specific peak facilitation was significantly larger than facilitation observed at a standardized longer-interval ISI of +10 ms. Our results suggest that unique insights can be provided with individual-specific measures of top-down effective connectivity mediated by direct and/or fastest-conducting pathways (indicated by the magnitude of facilitation observed at EFD 0 ms) and other descending pathways that encompass relatively slower and/or indirect connections from M1 to spinal circuits (indicated by peak facilitation and facilitation at longer ISIs). By comprehensively characterizing the temporal profile and inter-individual variability of descending modulation of spinal reflexes, our findings provide methodological guidelines and normative reference values to inform future studies on neurophysiological correlates of the complex array of descending neural connections between M1 and spinal circuits.
A Competitive Assay Based on Dual-Mode Au@Pt-DNA Biosensors for On-Site Sensitive Determination of Carbendazim Fungicide in Agricultural Products
Authors: Chen Ge , Zhai Rongqi , Liu Guangyang , Huang Xiaodong , Zhang Kaige , Xu Xiaomin , Li Lingyun , Zhang Yanguo , Wang Jing , Jin Maojun , Xu Donghui , Abd El-Aty A. M.
Published: February 2022

Carbendazim (CBZ), a systemic, broad-spectrum benzimidazole fungicide, is widely used to control fungal diseases in agricultural products. Its residues might pose risks to human health and the environment. Therefore, it is warranted to establish a rapid and reliable method for its residual quantification. Herein, we proposed a competitive assay that combined aptamer (DNA) specific recognition and bimetallic nanozyme gold@platinum (Au@Pt) catalysis to trace the CBZ residue. The DNA was labeled onto bimetallic nanozyme Au@Pt surface to produce Au@Pt probes (Au@Pt-DNA). The magnetic Fe3O4 was functionalized with a complementary strand of DNA (C-DNA) to form Fe3O4 probes (Fe3O4-C-DNA). Subsequently, the CBZ and the Fe3O4 probes competitively react with Au@Pt probes to form two Au@Pt-DNA biosensors (Au@Pt-ssDNA-CBZ and Au@Pt-dsDNA-Fe3O4). The Au@Pt-ssDNA-CBZ biosensor was designed for qualitative analysis through a naked-eye visualization strategy in the presence of CBZ. Meanwhile, Au@Pt-dsDNA-Fe3O4 biosensor was developed to quantitatively analyze CBZ using a multifunctional microplate reader. A competitive assay based on the dual-mode Au@Pt-DNA biosensors was established for onsite sensitive determination of CBZ. The limit of detection (LOD) and recoveries of the developed assay were 0.038 ng/mg and 71.88-110.11%, with relative standard deviations (RSDs) ranging between 3.15 and 10.91%. The assay demonstrated a good correlation with data acquired from liquid chromatography coupled with mass spectrometry/mass spectrometry analysis. In summary, the proposed competitive assay based on dual-mode Au@Pt-DNA biosensors might have a great potential for onsite sensitive detection of pesticides in agro-products.
Changes in Appetite-Dependent Hormones and Body Composition After 8 Weeks of High-Intensity Interval Training and Vitamin D Supplementation in Sedentary Overweight Men
Authors: Sheikholeslami-Vatani Dariush , Rostamzadeh Naser
Published: February 2022

Exercise and diet are important factors for energy balance and appetite regulation. The aim of this study was to investigate the effect of 8 weeks High Intensity Interval Training (HIIT) and vitamin D3 supplementation in sedentary overweight men. Forty-eight participants were randomly assigned to one of the following four groups (n = 12): HIIT + VitD, HIIT + placebo (3 sessions per week, 10 × 1 min interval cycling at 90–100% VO2peak separated by 1 min active recovery at 15% VO2peakfor 8 weeks), Vit D and control groups. Participants received 2,000 IU/day 25 (OH) D3 or placebo. Measurements were taken pre and post training after 10 h overnight fasting. Insulin, weight, BMI and body fat percentage were significantly decreased, but PYY was significantly increased in the HIIT + Vit D and HIIT + placebo groups (p = 0.001 and p = 0.001, respectively) after 8 weeks of HIIT. Insulin (p = 0.009, p = 0.001), weight, BMI and body fat percentage (p = 0.001, p = 0.001) were significantly lower in the HIIT + Vit D and HIIT + placebo groups compared to the Vit D and control groups. However, PYY was significantly higher in the HIIT + Vit D group compared to the Vit D (p = 0.025) and control groups (p = 0.007) and also in the HIIT + placebo group compared to the Vit D (p = 0.037) and control groups (p = 0.032) after 8 weeks of HIIT. The combination of regular HIIT with vitamin D supplementation has a effect on appetite control and body composition.
Effective Combinations of Immunotherapy and Radiotherapy for Cancer Treatment
Authors: Yu Siting , Wang Yang , He Ping , Shao Bianfei , Liu Fang , Xiang Zhongzheng , Yang Tian , Zeng Yuanyuan , He Tao , Ma Jiachun , Wang Xiran , Liu Lei
Published: February 2022

Though single tumor immunotherapy and radiotherapy have significantly improved the survival rate of tumor patients, there are certain limitations in overcoming tumor metastasis, recurrence, and reducing side effects. Therefore, it is urgent to explore new tumor treatment methods. The new combination of radiotherapy and immunotherapy shows promise in improving therapeutic efficacy and reducing recurrence by enhancing the ability of the immune system to recognize and eradicate tumor cells, to overcome tumor immune tolerance mechanisms. Nanomaterials, as new drug-delivery-system materials of the 21st century, can maintain the activity of drugs, improve drug targeting, and reduce side effects in tumor immunotherapy. Additionally, nanomaterials, as radiosensitizers, have shown great potential in tumor radiotherapy due to their unique properties, such as light, heat, electromagnetic effects. Here, we review the mechanisms of tumor immunotherapy and radiotherapy and the synergy of radiotherapy with multiple types of immunotherapies, including immune checkpoint inhibitors (ICIs), tumor vaccines, adoptive cell therapy, and cytokine therapy. Finally, we propose the potential for nanomaterials in tumor radiotherapy and immunotherapy.
The Concept, Development, and Application of a Home-Based High-Definition tDCS for Bilateral Motor Cortex Modulation in Migraine and Pain
Authors: DaSilva Alexandre F. , Datta Abhishek , Swami Jaiti , Kim Dajung J. , Patil Parag G. , Bikson Marom
Published: February 2022

Whereas, many debilitating chronic pain disorders are dominantly bilateral (e.g., fibromyalgia, chronic migraine), non-invasive and invasive cortical neuromodulation therapies predominantly apply unilateral stimulation. The development of excitatory stimulation targeting bilateral primary motor (M1) cortices could potentially expand its therapeutic effect to more global pain relief. However, this is hampered by increased procedural and technical complexity. For example, repetitive transcranial magnetic stimulation (rTMS) and 4 × 1/2 × 2 high-definition transcranial direct current stimulation (4 × 1/2 × 2 HD-tDCS) are largely center-based, with unilateral-target focus—bilateral excitation would require two rTMS/4 × 1 HD-tDCS systems. We developed a system that allows for focal, non-invasive, self-applied, and simultaneous bilateral excitatory M1 stimulation, supporting long-term home-based treatment with a well-tolerated wearable battery-powered device. Here, we overviewed the most employed M1 neuromodulation methods, from invasive techniques to non-invasive TMS and tDCS. The evaluation extended from non-invasive diffuse asymmetric bilateral (M1-supraorbital [SO] tDCS), non-invasive and invasive unilateral focal (4 × 1/2 × 2 HD-tDCS, rTMS, MCS), to non-invasive and invasive bilateral bipolar (M1-M1 tDCS, MCS), before outlining our proposal for a neuromodulatory system with unique features. Computational models were applied to compare brain current flow for current laboratory-based unilateral M11 and bilateral M12 HD-tDCS models with a functional home-based M11−2 HD-tDCS prototype. We concluded the study by discussing the promising concept of bilateral excitatory M1 stimulation for more global pain relief, which is also non-invasive, focal, and home-based.
Efficacy and Safety of Finerenone in Chronic Kidney Disease: A Systematic Review and Meta-Analysis of Randomized Clinical Trials
Authors: Zhang Ming-Zhu , Bao Wujisiguleng , Zheng Qi-Yan , Wang Ya-Hui , Sun Lu-Ying
Published: February 2022

Background: Chronic kidney disease (CKD) is a global public health issue. In recent years, the effectiveness of finerenone for treatment of CKD has been the subject of considerable debate. The main objective of the current meta-analysis was to validate the clinical efficacy and safety of finerenone in patients with CKD.

Methods: Seven databases were searched for randomized controlled trials (RCTs) comparing finerenone with placebo in patients with CKD. Data from eligible studies were extracted, and the Cochrane risk of bias tool utilized for evaluating the methodological quality of RCTs. The effect size was estimated using the risk ratio (RR) and mean difference (MD) with 95% confidence interval (CI).

Results: Five trials (n = 13,078) were included. Compared to placebo groups, the urinary albumin-to-creatinine ratio (UACR) mean from the baseline was significantly lower [MD −0.30 (95% CI −0.32, −0.28), p < 0.00001], while a decrease in the estimated glomerular filtration rate (eGFR) from baseline was significantly higher [MD −2.44 (95% CI −2.82, −2.05), p < 0.00001] for the finerenone groups. Furthermore, the proportion of patients with decreased eGFR (≥40%) post-baseline was significantly lower [RR 0.85 (95% CI 0.78, 0.93), p = 0.0002], along with end-stage kidney disease (ESKD) [RR 0.80 (95% CI 0.65, 0.99), p = 0.04] and cardiovascular events (CVs) [RR 0.88 (95% CI 0.80, 0.95), p < 0.003] in the finerenone groups. In terms of safety, the increase in the serum potassium concentration and incidence of hyperkalemia was significantly higher for the finerenone groups [MD 0.17 (95% CI 0.10, 0.24), p < 0.00001; RR 2.03 (95% CI 1.83, 2.26), p < 0.00001, respectively], but the incidence of adverse events (AEs) was similar to placebo [RR 1.00 (95% CI 0.98–1.01), p = 0.67]. In all cases, the results were rated as providing moderate-quality or high-quality evidence.

Conclusion: Data from our meta-analysis suggest that finerenone confers significant renal and cardiovascular benefits in patients with CKD. While higher risk of hyperkalemia was observed with finerenone than placebo, differences in AEs were not significant. Finerenone may therefore present a novel promising therapeutic agent for patients with CKD.

Systematic Review Registration: [https://inplasy.com/inplasy-2021-9-0020/], identifier [INPLASY202190020].
Reversed Apico-Basal Myocardial Relaxation Sequence During Exercise in Long QT Syndrome Mutations Carriers With History of Previous Cardiac Events
Authors: Charisopoulou Dafni , Koulaouzidis George , Rydberg Annika , Henein Michael Y.
Published: February 2022

Background

Recent echocardiography studies in inherited long QT syndrome (LQTS) have shown left ventricular (LV) myocardial relaxation disturbances to follow markedly prolonged and dispersed mechanical contraction.

Aim

We used speckle-tracking echocardiography to assess disturbances in LV myocardial relaxation sequence during exercise and their relationship to symptoms.

Methods

Forty seven LQTS patients (45 ± 15 years, 25 female and 20 symptomatic, LVEF: 65 ± 6%) and 35 controls underwent exercise echocardiogram using Bruce protocol. ECG and echo parameters were recorded at rest, peak exercise (p.e.) and recovery.

Results

Between patients and controls there were no differences in age, gender, HR or LVEF. At p.e, patients had longer time to LV longitudinal ESR(tESR) at all three LV segments; basal (p< 0.0001), mid- cavity (p= 0.03) and apical (p= 0.03) whereas at rest such difference was noted only at base (p= 0.0007). Patients showed reversed apico-basal relaxation sequence (ΔtESRbase–apex) with early relaxation onset occurring later at base than at apex, both at rest (49 ± 43 vs. –29 ± 19 ms,p< 0.0001) and at p.e. (46 ± 38 vs. –40 ± 22 ms,p< 0.0001), particularly in symptomatic patients (69 ± 44 vs. 32 ± 26,p< 0.0007). ΔtESRbase–apexcorrelated with longer QTc interval, lower ESRand attenuated LV stroke volume.

Conclusion

LQTS patients show reversed longitudinal relaxation sequence, which worsens with exercise, particularly in those with previous cardiac events.
Spotlight on the Binding Affinity of Ion Channels for Phosphoinositides: From the Study of Sperm Flagellum
Authors: Kawai Takafumi , Okamura Yasushi
Published: February 2022

The previous studies revealed that many types of ion channels have sensitivity to PtdIns(4,5)P2, which has been mainly shown using heterologous expression system. On the other hand, there remains few evidence showing that PtdIns(4,5)P2 natively regulate the ion channel activities in physiological context. Our group recently discovered that a sperm specific K+ channel, Slo3, is natively regulated by PtdIns(4,5)P2 in sperm flagellum. Very interestingly, a principal piece, to which Slo3 specifically localized, had extremely low density of PtdIns(4,5)P2 compared to the regular cell plasma membrane. Furthermore, our studies and the previous ones also revealed that Slo3 had much stronger PtdIns(4,5)P2 affinity than KCNQ2/3 channels, which are widely regulated by endogenous PtdIns(4,5)P2 in neurons. Thus, the high-PtdIns(4,5)P2 affinity of Slo3 is well-adapted to the specialized PtdIns(4,5)P2 environment in the principal piece. This study sheds light on the relationship between PtdIns(4,5)P2-affinity of ion channels and their PtdIns(4,5)P2 environment in native cells. We discuss the current understanding about PtdIns(4,5)P2 affinity of diverse ion channels and their possible regulatory mechanism in native cellular environment.
Adolescents' Resilience During COVID-19 Pandemic and Its Mediating Role in the Association Between SEL Skills and Mental Health
Authors: Grazzani Ilaria , Agliati Alessia , Cavioni Valeria , Conte Elisabetta , Gandellini Sabina , Lupica Spagnolo Mara , Ornaghi Veronica , Rossi Francesca Micol , Cefai Carmel , Bartolo Paul , Camilleri Liberato , Oriordan Mollie Rose
Published: February 2022

The main purpose of this paper is to investigate the role of social and emotional learning (SEL) skills and resilience in explaining mental health in male and female adolescents, during the COVID-19 pandemic. Three self-report questionnaires were administered to 778 participants aged between 11 and 16 years (mean age = 12.73 years; SD = 1.73) and recruited from 18 schools in Northern Italy. The SSIS-SELb-S and the CD-RISC 10 assessed SEL and resilience skills respectively, while the Strengths and Difficulties Questionnaire (SDQ) was used to measure mental health in terms of internalizing problems, externalizing problems, and prosocial behavior. We found that SEL and resilience skills were positively and significantly associated with each other, negatively associated with internalizing and externalizing problems, and positively related to prosocial behavior. Three linear regression analyses showed the significant role of resilience, age, and gender in explaining the variance of internalizing problems; the significant role of SEL skills, resilience, age, and gender in explaining the variance of externalizing problems; and the role of SEL skills, age, and gender in explaining prosocial behavior. Importantly, we found that resilience fully mediated the relationship between SEL skills and internalizing problems, partially mediated the relationship between SEL skills and externalizing problems and didn't mediate the relationship between SEL skills and prosocial behavior. The paper concludes with a discussion of the limitations of the study as well as its practical implications.
Effects of COVID-19 on Mental Health and Its Relationship With Death Attitudes and Coping Styles Among Hungarian, Norwegian, and Turkish Psychology Students
Authors: Oker Kemal , Reinhardt Melinda , Schmelowszky Ágoston
Published: February 2022

The purpose of this study was to investigate mental effects of coronavirus disease 2019 (COVID-19) and its relationship with death attitudes and coping styles among Hungarian, Norwegian, and Turkish psychology students. A total of 388 participants from Hungary (N = 122, 31.4%), Norway (N = 96, 24.7%), and Turkey (N = 170, 43.8%) were recruited during the pandemic. The Depression, Anxiety and Stress Scale, the Impact of Event Scale-Revised, the Carver Brief COPE Inventory, and the Death Attitude Profile-Revised were used. The results indicated that escape acceptance might be the most maladaptive death attitude during COVID-19, as it was related to poorer mental health among the Hungarian, Norwegian, and Turkish psychology students. Self-blame, behavioral disengagement, self-distraction, and substance use coping styles were also related to poorer mental health, whereas positive-reframing (only among the Hungarian and Turkish participants) and humor (only among the Norwegian participants) were related to better mental health among our sample in the context of COVID-19. The findings implied that death attitudes and coping styles may differ in their efficacy among the Hungarian, Norwegian, and Turkish participants. These differences were discussed in detail in the discussion part. During the pandemic, practitioners might pay closer attention to patients with higher escape acceptance death attitude and patients who use dysfunctional coping styles. Additionally, patients can be encouraged to use techniques involving positive reframing and humor coping styles.
Interplay Between the Salience and the Default Mode Network in a Social-Cognitive Task Toward a Close Other
Authors: Ribeiro da Costa Cátia , Soares Jose M. , Oliveira-Silva Patrícia , Sampaio Adriana , Coutinho Joana F.
Published: February 2022

Social cognition relies on two main subsystems to construct the understanding of others, which are sustained by different social brain networks. One of these social networks is the default mode network (DMN) associated with the socio-cognitive subsystem (i.e., mentalizing), and the other is the salience network (SN) associated with the socio-affective route (i.e., empathy). The DMN and the SN are well-known resting state networks that seem to constitute a baseline for the performance of social tasks. We aimed to investigate both networks' functional connectivity (FC) pattern in the transition from resting state to social task performance. A sample of 38 participants involved in a monogamous romantic relationship completed a questionnaire of dyadic empathy and underwent an fMRI protocol that included a resting state acquisition followed by a task in which subjects watched emotional videos of their romantic partner and elaborated on their partner's (Other condition) or on their own experience (Self condition). Independent component and ROI-to-ROI correlation analysis were used to assess alterations in task-independent (Rest condition) and task-dependent (Self and Other conditions) FC. We found that the spatial FC maps of the DMN and SN evidenced the traditional regions associated with these networks in the three conditions. Anterior and posterior DMN regions exhibited increased FC during the social task performance compared to resting state. The Other condition revealed a more limited SN's connectivity in comparison to the Self and Rest conditions. The results revealed an interplay between the main nodes of the DMN and the core regions of the SN, particularly evident in the Self and Other conditions.
An Evaluation of an Online Training Platform for Teaching Positive Emotions for People With Schizophrenia
Authors: Nguyen Alexandra , Frobert Laurent , Kollbrunner Aurélien , Favrod Jérôme
Published: February 2022

Background

The dissemination of new interventions in clinical practice remains challenging. E-learning may provide wide access in various settings and allow tailored learning trajectories and an adapted training pace. This study evaluates an online platform to train professionals to lead the Positive Emotion Program for Schizophrenia (PEPS) for patients with anhedonia. This study aims to test the reception provided by clinicians to the platform and its perceived usefulness and investigate whether e-PEPS training improves knowledge about the facilitation of PEPS.

Materials and Methods

Participants were recruited through advertisements. All participants provided their informed consent on a registration form and completed two pre-test questionnaires, a knowledge test on negative symptoms in schizophrenia, learning strategies and the partnership relationship, and a test on the ability to savor pleasant moments. After the training, they completed the same questionnaire and an evaluation form of the training and its application in personal and professional life.

Results

Two-hundred and ten participants were registered to participate into the study, 185 received the access to the platform, and 101 participants completed the training and the post-test assessments. Satisfaction with training was high. The results showed that the participants significantly improved their knowledge about PEPS and increased the skills taught in their personal repertoire after the training. The training allows most clinicians to plan to lead a PEPS group in the year following training.

Discussion

As a result of this study, training has been improved and is now freely available to all interested clinicians.
Long-Term Air Pollution Exposure and Ischemic Heart Disease Mortality Among Elderly in High Aging Asian Economies
Authors: Mumtaz Ayesha , Rehman Nadia , Haider Aftab , Rehman Shazia
Published: February 2022

In the epidemiological literature, the impact of environmental pollution on cardiac mortality has been well documented. There is, however, a paucity of evidence on the impact of air pollution exposure on ischemic heart disease (IHD) mortality among the Asian aged population. In response, this research seeks to investigate the degree of proximity between exposure to ambient PM2.5, household PM2.5, ground-level ozone (O3), and IHD mortality in the top seven Asian economies with the highest aging rates. This investigation is held in two phases. In the first phase, grey modeling is employed to assess the degree of proximity among the selected variables, and then rank them based on their estimated grey weights. In addition, a grey-based Technique for Order of Preference by Similarity to Ideal Solution (G-TOPSIS) is adopted to identify the key influencing factor that intensifies IHD mortality across the selected Asian economies. According to the estimated results, South Korea was the most afflicted nation in terms of IHD mortality owing to ambient PM2.5 and ground-level O3 exposure, whereas among the studied nations India was the biggest contributor to raising IHD mortality due to household PM2.5 exposure. Further, the outcomes of G-TOPSIS highlighted that exposure to household PM2.5 is a key influencing risk factor for increased IHD mortality in these regions, outweighing all other air pollutants. In conclusion, this grey assessment may enable policymakers to target more vulnerable individuals based on scientific facts and promote regional environmental justice. Stronger emission regulations will also be required to mitigate the adverse health outcomes associated with air pollution exposure, particularly in regions with a higher elderly population.
Effort-Reward Imbalance in Emergency Department Physicians: Prevalence and Associated Factors
Authors: Tian Mengge , Zhou Xuan , Yin Xiaoxv , Jiang Nan , Wu Yafei , Zhang Jiali , Lv Chuanzhu , Gong Yanhong
Published: February 2022

Objectives

To examine the prevalence of effort-reward imbalance and explore its associated factors among emergency department physicians in China.

Methods

A cross-sectional survey was conducted in the Chinese emergency department in 2018. A total of 10,457 emergency department physicians completed a structured questionnaire containing demographic characteristics, work-related data, and effort-reward imbalance scale. All the data were analyzed using descriptive analysis and stepwise logistic regression.

Results

The prevalence of effort-reward imbalance was 78.39% among emergency department physicians in China. The results showed that the male emergency department physicians with a bachelor's degree, an intermediate title, long years of service, a high frequency of night shift, and who suffered workplace violence were at a higher risk of effort-reward imbalance. In contrast, physicians with higher monthly income and perceived adequate staff were associated with a lower risk of effort-reward imbalance.

Conclusions

The situation of effort-reward imbalance was serious among emergency department physicians in China. Administrators should pay more attention to key groups and take measures from the perspectives of effort and reward to improve the effort-reward imbalance in emergency department physicians.
Can China's New Rural Cooperative Medical System Improve Farmers' Subjective Well-Being?
Authors: Qi Wenhao , Liu Fang , Zhang Tian , Qi Xiulin
Published: February 2022

The New Rural Cooperative Medical System (NRCMS) is one of the essential systems for ensuring public health in rural China. This paper investigates the effect of farmers' participation in the NRCMS on their subjective well-being and its mechanisms using data from the Chinese General Social Survey 2017. The results show that farmers' participation in the NRCMS significantly enhances their subjective well-being, and these results remain robust after regression with the instrumental variables method and propensity score matching method. Further analysis of the mechanisms suggests that participation in the NRCMS can enhance farmers' subjective well-being by increasing their consumption levels other than medical consumption. Moreover, medical consumption levels play a negative role in participating in the NRCMS on farmers' subjective well-being, which can be explained as the “masking effect.” The regression results of the subsamples show that the higher a farmer's income is, the less his or her participation in the NRCMS enhances subjective well-being. And the effect of participation in the NRCMS on farmers' subjective well-being is not significant if their health status is too high or too low.
Low Dose CT Denoising by ResNet With Fused Attention Modules and Integrated Loss Functions
Authors: Marcos Luella , Alirezaie Javad , Babyn Paul
Published: February 2022

X-ray computed tomography (CT) is a non-invasive medical diagnostic tool that has raised public concerns due to the associated health risks of radiation dose to patients. Reducing the radiation dose leads to noise artifacts, making the low-dose CT images unreliable for diagnosis. Hence, low-dose CT (LDCT) image reconstruction techniques have offered a new research area. In this study, a deep neural network is proposed, specifically a residual network (ResNet) using dilated convolution, batch normalization, and rectified linear unit (ReLU) layers with fused spatial- and channel-attention modules to enhance the quality of LDCT images. The network is optimized using the integration of per-pixel loss, perceptual loss via VGG16-net, and dissimilarity index loss. Through an ablation experiment, these functions show that they could effectively prevent edge oversmoothing, improve image texture, and preserve the structural details. Finally, comparative experiments showed that the qualitative and quantitative results of the proposed network outperform state-of-the-art denoising models such as block-matching 3D filtering (BM3D), Markovian-based patch generative adversarial network (patch-GAN), and dilated residual network with edge detection (DRL-E-MP).
Effects of Different Supplemental Levels of Eucommia ulmoides Leaf Extract in the Diet on Carcass Traits and Lipid Metabolism in Growing–Finishing Pigs
Authors: Yang Yuhuan , Li Fengna , Guo Qiuping , Wang Wenlong , Zhang Lingyu , Yin Yunju , Gong Saiming , Han Mengmeng , Yin Yulong
Published: February 2022

This study examined the effects of dietary Eucommia ulmoides leaf extract (ELE) supplements on carcass traits and lipid metabolism in growing–finishing pigs. A total of 144 crossbred (Duroc × Landrace × Yorkshire) piglets with an average initial weight of 10.11 ± 0.03 kg were randomly allotted to four treatment groups, each with six replicates and six piglets per replicate. Each group of pigs was fed a basal diet or a diet supplemented with increasing levels of ELE (0.1, 0.2, or 0.3%). The results showed that adding ELE had no negative effect on the growth performance of pigs. Dietary supplements of 0.1% ELE significantly increased carcass weight (p < 0.01), dressing percentage (p < 0.01), carcass length (p < 0.05), and eye muscle area (p < 0.05). Compared with the control group, a 0.2% ELE supplement significantly increased (p < 0.01) the levels of adiponectin, insulin-like growth factor 1, and hormone-sensitive lipase and lipoprotein lipase activity in the serum. Histological examination showed that ELE inhibited fat deposition in the backfat tissue. Lipid metabolism-related biochemical indices and mRNA expression levels were improved after supplementing diets with ELE. Moreover, all three levels of ELE dramatically upregulated (p < 0.05) the protein levels of p-AMPK-α and p-ACC. In summary, adding ELE to pig diets could improve the carcass traits of growing–finishing pigs and exert a lipid-lowering effect by activating the AMPK-ACC pathway and regulating mRNA expression levels related to lipid metabolism. Supplementing the diet with 0.1–0.2% ELE is the optimal range to reduce fat deposition in pig backfat tissue.
Predictions of the SARS-CoV-2 Omicron Variant (B.1.1.529) Spike Protein Receptor-Binding Domain Structure and Neutralizing Antibody Interactions
Authors: Ford Colby T. , Jacob Machado Denis , Janies Daniel A.
Published: February 2022

The genome of the SARS-CoV-2 Omicron variant (B.1.1.529) was released on November 22, 2021, which has caused a flurry of media attention due the large number of mutations it contains. These raw data have spurred questions around vaccine efficacy. Given that neither the structural information nor the experimentally-derived antibody interaction of this variant are available, we have turned to predictive computational methods to model the mutated structure of the spike protein's receptor binding domain and posit potential changes to vaccine efficacy. In this study, we predict some structural changes in the receptor-binding domain that may reduce antibody interaction without completely evading existing neutralizing antibodies (and therefore current vaccines).
COVID-19 in German Competitive Sports: Protocol for a Prospective Multicenter Cohort Study (CoSmo-S)
Authors: Niess Andreas Michael , Widmann Manuel , Gaidai Roman , Gölz Christian , Schubert Isabel , Castillo Katty , Sachs Jan Philipp , Bizjak Daniel , Vollrath Shirin , Wimbauer Fritz , Vogel Azin , Keller Karsten , Burgstahler Christof , Quermann Anne , Kerling Arno , Schneider Gerald , Zacher Jonas , Diebold Katharina , Grummt Maximilian , Beckendorf Claudia , Buitenhuis Johannes , Egger Florian , Venhorst Andreas , Morath Oliver , Barsch Friedrich , Mellwig Klaus-Peter , Oesterschlink Julian , Wüstenfeld Jan , Predel Hans-Georg , Deibert Peter , Friedmann-Bette Birgit , Mayer Frank , Hirschmüller Anja , Halle Martin , Steinacker Jürgen Michael , Wolfarth Bernd , Meyer Tim , Böttinger Erwin , Flechtner-Mors Marion , Bloch Wilhelm , Haller Bernhard , Roecker Kai , Reinsberger Claus
Published: February 2022

Objective: It is unclear whether and to what extent COVID-19 infection poses health risks and a chronic impairment of performance in athletes. Identification of individual health risk is an important decision-making basis for managing the pandemic risk of infection with SARS-CoV-2 in sports and return to play (RTP).

Methods: This study aims 1) to analyze the longitudinal rate of seroprevalence of SARS-CoV-2 in German athletes, 2) to assess health-related consequences in athletes infected with SARS-CoV-2, and 3) to reveal effects of the COVID-19 pandemic in general and of a cleared SARS-CoV-2 infection on exercise performance. CoSmo-S is a prospective observational multicenter study establishing two cohorts: 1) athletes diagnosed positive for COVID-19 (cohort 1) and 2) federal squad athletes who perform their annual sports medical preparticipation screening (cohort 2). Comprehensive diagnostics including physical examination, laboratory blood analyses and blood biobanking, resting and exercise electrocardiogram (ECG), echocardiography, spirometry and exercise testing added by questionnaires are conducted at baseline and follow-up.

Results and Conclusion: We expect that the results obtained, will allow us to formulate recommendations regarding RTP on a more evidence-based level.
The Influence of Diabetes Mellitus on the Risks of End-Stage Kidney Disease and Mortality After Liver Transplantation
Authors: Lee Chung-Ying , Wu Mei-Yi , Chan Hsiu-Chen , Chen Tzu-Ting , Hsu Le-Yin , Wu Mai-Szu , Cherng Yih-Giun
Published: February 2022

This retrospective study aimed to investigate the effect of diabetes mellitus (DM) on the risks of end-stage kidney disease (ESKD) and post-liver transplantation (post-LT) mortality. Using data from the National Health Insurance Research Database, Taiwan, 3,489 patients who received a LT between 1 January 2005, and 31 December 2015, were enrolled in this study and divided into the pre-existing DM, post-LT DM (PLTDM), and without DM groups. All subjects were followed up from 1 year after LT to the index date for ESKD, and the occurrence of death, or until 31 December 2016. Of the 3,489 patients with LT, 1,016 had pre-existing DM, 215 had PLTDM, and 2,258 had no DM pre- or post-LT. The adjusted HRs of ESKD were 1.77 (95% Confidence Interval [CI], .78–3.99) and 2.61 (95% CI, 1.63–4.18) for PLTDM group and pre-existing DM group compared to without DM group, respectively. For the risk of death, the adjusted HRs were 1.05 (95% CI, .72–1.55) and 1.28 (95% CI, 1.04–1.59) for PLTDM group and pre-existing DM group compared to those without DM group, respectively. The sensitivity analysis for the risk of ESKD and death also revealed the consistent result. Pre-existing DM has significant increase the risk of post-LT ESKD and mortality. The role of PLTDM should be explored to explain postoperative morbidity and mortality.
Impact of Subsoiling on Cultivated Horizon Construction and Grain Yield of Winter Wheat in the North China Plain
Authors: Qiang Xiaoman , Sun Jingsheng , Ning Huifeng
Published: February 2022

In order to explore the effects of subsoiling tillage measures on the construction of soil cultivated horizon and the yield and water use efficiency of winter wheat in the North China Plain, three tillage methods, including no tillage (PZ), rotary tillage (PR), and subsoiling (PS), combined with straw returning measures were implemented in the winter wheat season in Xinxiang, Henan Province from 2016 to 2018. The effects of tillage measures on the improvement of cultivated land quality and the water saving and yield increase of winter wheat were investigated. The results showed that compared with no-tillage treatment, subsoiling significantly reduced soil bulk density by 8.88% and increased soil porosity by 13.04% in 20–40 cm soil layer; significantly reduced soil compaction by 56.96% in 0–40 cm soil layer; subsoiling combined with straw returning significantly increased soil organic carbon content in plough layer of winter wheat, whereas rotary tillage decreased soil organic carbon content. Subsoiling is more conducive to soil moisture movement to the deep layer after irrigation or rainfall, and the water consumption of subsoiling is the largest in the whole growth period of winter wheat. Subsoiling could better coordinate the relationship between water consumption and yield, which increased yield by 34.48–38.10% and water use efficiency by 19.57–21.96% compared with no-tillage treatment, respectively. Therefore, subsoiling before sowing combined with straw returning was beneficial to the reasonable construction of soil cultivated horizon, and significantly improved the yield and water use efficiency of winter wheat under the climatic conditions in the North China Plain.
Use of Digestate as an Alternative to Mineral Fertilizer: Effects on Soil Mineral Nitrogen and Winter Wheat Nitrogen Accumulation in Clay Loam
Authors: Petraityte Danute , Arlauskiene Ausra , Ceseviciene Jurgita
Published: February 2022

Knowledge of the mineralisation and nutrient release of organic fertilisers is essential to ensure plant nutrient demand and availability, to increase N use efficiency and to minimise environmental risks. In 2018–2020, two similar field experiments were carried out on clay loam Cambisol with winter wheat (Triticum aestivum L.) grown without N application and applying liquid anaerobic digestate (LD), pig slurry (PS) and ammonium nitrate (AN) fertilizer with and without additional fertilization (N120 and N120+50). The aim of the research was to compare the effect of organic and mineral fertilizers on the variation of soil mineral nitrogen forms in the 0–30, 30–60 cm soil layers and N accumulation in wheat yield. Fertilizers applied during the previous growing season increased the nitrate and ammonium nitrogen (N-NO3 and N-NH4) content after the resumption of winter wheat vegetation. The dry period in spring (2019) had a negative impact on winter wheat N uptake. In a year of normal moisture content (2020), PS and LD fertilizers and the fertilizer application of the previous year (2019) significantly increased the N-NO3 content in the topsoil, while all applied fertilizers increased it in the deeper soil layer (by a factor of between 3.6 and 12.3), compared to unfertilized soil.
Beneficial Effects of Silicon Fertilizer on Growth and Physiological Responses in Oil Palm
Authors: Duangpan Saowapa , Tongchu Yanipha , Hussain Tajamul , Eksomtramage Theera , Onthong Jumpen
Published: February 2022

Vigorous and well-established nursery seedlings are an important component of sustainable oil palm production. We postulated that Si fertilization at the seedling stage could help to achieve improved performance of oil palm seedlings leading to healthy and vigorous nursery establishment. In this study, we evaluated the growth and physiological responses of oil palm Tenera hybrid seedlings under three Si fertilization treatments and a control including (i) 0 g Ca2SiO4 (T0), (ii) 0.5 g Ca2SiO4 (T1), (iii) 3.5 g Ca2SiO4 (T2), and (iv) 7.0 g Ca2SiO4 (T3) per plant per month. Ca2SiO4 was used as the Si fertilizer source and was applied for four consecutive months. Nondestructive data including stem diameter, plant height, leaf length, photosynthetic rate, leaf angle, and leaf thickness and destructive data including leaf, stem, and root fresh weight and dry weight, as well as chlorophyll a, Si, and nitrogen contents, were recorded before treatment (0 DAT), as well as 60 (60 DAT) and 120 days after treatment (120 DAT). Results indicated that Si fertilization enhanced Si accumulation in oil palm seedlings, and maximum accumulation was observed in the aerial parts especially the leaves with the highest accumulation of 0.89 % dry weight at T3. Higher Si accumulation stimulated the growth of seedlings; a total fresh weight of 834.28 g and a total dry weight of 194.34 g were observed at T3. Chlorophyll a content (0.83 gm−2) and net photosynthetic rate (4.98 µM CO2·m−2·s−1) were also observed at T3. Leaf morphology was not significantly influenced under Si fertilization, whereas the nitrogen content of seedlings was significantly increased. Correlation analysis revealed a highly significant and positive association among Si accumulation, chlorophyll a content, photosynthetic rate, total fresh weight, total dry weight, and nitrogen content of seedlings, indicating that Si fertilization enhanced the performance of these attributes. On the basis of the research evidence, it was concluded that Si fertilization should be considered for improved nutrient management for oil palm seedling and nursery production.
Evaluation of Strategies to Improve the Environmental and Economic Sustainability of Cow–Calf Production Systems
Authors: Lancaster Phillip A. , Larson Robert L.
Published: February 2022

Grazing cow–calf production systems account for 60 to 70% of the greenhouse gas emissions of U.S. beef production. The objective of this analysis was to evaluate the importance of management strategies (cow maintenance energy requirements, reproductive efficiency, forage nutritive value, and forage yield) on the sustainability of cow–calf production systems using a sensitivity analysis in a production systems model. The Beef Cattle Systems Model was used to simulate a cow–calf production system in the Kansas Flint Hills using Angus genetics over a 24 year time period. The model was modified to create variation among cow herds in the base net energy for the maintenance requirement (NEm_Req), postpartum interval (PPI), grazed forage digestibility (Forage_TDN), and forage yield per hectare (Forage_Yield). The model was run for 1000 iterations/herds of a 100-cow herd. A stepwise regression analysis in conjunction with standardized regression analysis was used to identify important predictors of an indicator of greenhouse gas (GHG) emission intensity, dry matter intake per kilogram weaned, and two indicators of economic sustainability, winter feed use and returns over variable costs, using R statistical software. The most important predictor of DMI per kilogram weaned was calf weaning weight followed by NEm_Req, whereas returns over variable costs were primarily influenced by kilograms weaned per cow exposed and total purchased feed (supplement + winter feed), which were strongly influenced by NEm_Req and Forage_Yield, respectively. In conclusion, decreasing the net energy required for maintenance improved both economic and environmental sustainability, and increasing forage yield and length of the grazing season improved economic sustainability, implying that these strategies should be primary targets to enhance the sustainability of cow–calf production systems.