bluuebunny/crossref_metadata_2025 huggingface dataset:
Mitochondrial Signaling Pathways Associated with DNA Damage Responses
Authors: Shimura Tsutomu
Published: March 2023

Under physiological and stress conditions, mitochondria act as a signaling platform to initiate biological events, establishing communication from the mitochondria to the rest of the cell. Mitochondrial adenosine triphosphate (ATP), reactive oxygen species, cytochrome C, and damage-associated molecular patterns act as messengers in metabolism, oxidative stress response, bystander response, apoptosis, cellular senescence, and inflammation response. In this review paper, the mitochondrial signaling in response to DNA damage was summarized. Mitochondrial clearance via fusion, fission, and mitophagy regulates mitochondrial quality control under oxidative stress conditions. On the other hand, damaged mitochondria release their contents into the cytoplasm and then mediate various signaling pathways. The role of mitochondrial dysfunction in radiation carcinogenesis was discussed, and the recent findings on radiation-induced mitochondrial signaling and radioprotective agents that targeted mitochondria were presented. The analysis of the mitochondrial radiation effect, as hypothesized, is critical in assessing radiation risks to human health.
Genome-Wide Characterization and Expression Analysis of Transcription Factor Families in Desert Moss Syntrichia caninervis under Abiotic Stresses
Authors: Salih Haron , Bai Wenwan , Zhao Mingqi , Liang Yuqing , Yang Ruirui , Zhang Daoyuan , Li Xiaoshuang
Published: March 2023

Transcription factor (TF) families play important roles in plant stress responses. S. caninervis is a new model moss for plant desiccation tolerance studies. Here, we report a high-confidence identification and characterization of 591 TFs representing 52 families that covered all chromosomes in S. caninervis. GO term and KEGG pathway analysis showed that TFs were involved in the regulation of transcription, DNA-templated, gene expression, binding activities, plant hormone signal transduction, and circadian rhythm. A number of TF promoter regions have a mixture of various hormones-related cis-regulatory elements. AP2/ERF, bHLH, MYB, and C2H2-zinc finger TFs were the overrepresented TF families in S. caninervis, and the detailed classification of each family is performed based on structural features. Transcriptome analysis revealed the transcript abundances of some ScAP2/ERF, bHLH, MYB, and C2H2 genes were accumulated in the treated S. caninervis under cold, dehydration, and rehydration stresses. The RT-qPCR results strongly agreed with RNA-seq analysis, indicating these TFs might play a key role in S. caninervis response to abiotic stress. Our comparative TF characterization and classification provide the foundations for functional investigations of the dominant TF genes involved in S. caninervis stress response, as well as excellent stress tolerance gene resources for plant stress resistance breeding.
Prospective Roles of Tumor Necrosis Factor-Alpha (TNF-α) in COVID-19: Prognosis, Therapeutic and Management
Authors: Mohd Zawawi Zarina , Kalyanasundram Jeevanathan , Mohd Zain Rozainanee , Thayan Ravindran , Basri Dayang Fredalina , Yap Wei Boon
Published: March 2023

The coronavirus disease 2019 (COVID-19) became a worldwide concern at the beginning of 2020 and has affected millions. Several previous studies revealed the impact of the imbalanced innate immune response on the progression of COVID-19 and its disease outcomes. High levels of proinflammatory cytokines such as tumor necrosis factor-alpha (TNF-α) and interleukins are produced readily by innate immune cells to fight Severe Acute Respiratory Syndrome-Coronavirus-2 (SARS-CoV-2) infections. Nonetheless, cytokine-mediated inflammatory events are also linked to detrimental lung injury and respiratory failure, which can result in deaths among COVID-19 patients. TNF-α is amongst the early cytokines produced to mediate proinflammatory responses and enhance immune cell infiltration in response to SARS-CoV-2 infections. In COVID-19, TNF-α-mediated inflammation can cause detrimental tissue damage and gradually promotes lung fibrosis, which later results in pneumonia, pulmonary edema, and acute respiratory distress syndrome. This review, therefore, aims to deliberate the immunomodulatory roles of TNF-α in promoting inflammation and its relation with COVID-19 morbidity and mortality. In addition, this review also proposes the potential of TNF-α as a biomarker for the prognosis of severe COVID-19 and its related complications and as a molecular target for anti-TNF-α therapy.
Med25 Limits Master Regulators That Govern Adipogenesis
Authors: Saunders Jasmine , Sikder Kunal , Phillips Elizabeth , Ishwar Anurag , Mothy David , Margulies Kenneth B. , Choi Jason C.
Published: March 2023

Mediator 25 (Med25) is a member of the mediator complex that relays signals from transcription factors to the RNA polymerase II machinery. Multiple transcription factors, particularly those involved in lipid metabolism, utilize the mediator complex, but how Med25 is involved in this context is unclear. We previously identified Med25 in a translatome screen of adult cardiomyocytes (CMs) in a novel cell type-specific model of LMNA cardiomyopathy. In this study, we show that Med25 upregulation is coincident with myocardial lipid accumulation. To ascertain the role of Med25 in lipid accumulation, we utilized iPSC-derived and neonatal CMs to recapitulate the in vivo phenotype by depleting lamins A and C (lamin A/C) in vitro. Although lamin A/C depletion elicits lipid accumulation, this effect appears to be mediated by divergent mechanisms dependent on the CM developmental state. To directly investigate Med25 in lipid accumulation, we induced adipogenesis in Med25-silenced 3T3-L1 preadipocytes and detected enhanced lipid accumulation. Assessment of pertinent mediators driving adipogenesis revealed that C/EBPα and PPARγ are super-induced by Med25 silencing. Our results indicate that Med25 limits adipogenic potential by suppressing the levels of master regulators that govern adipogenesis. Furthermore, we caution the use of early-developmental-stage cardiomyocytes to model adult-stage cells, particularly for dissecting metabolic perturbations emanating from LMNA mutations.
Molecular Docking Identifies 1,8-Cineole (Eucalyptol) as A Novel PPARγ Agonist That Alleviates Colon Inflammation
Authors: Venkataraman Balaji , Almarzooqi Saeeda , Raj Vishnu , Bhongade Bhoomendra A. , Patil Rajesh B. , Subramanian Veedamali S. , Attoub Samir , Rizvi Tahir A. , Adrian Thomas E. , Subramanya Sandeep B.
Published: March 2023

Inflammatory bowel disease, comprising Crohn’s disease (CD) and ulcerative colitis (UC), is often debilitating. The disease etiology is multifactorial, involving genetic susceptibility, microbial dysregulation, abnormal immune activation, and environmental factors. Currently, available drug therapies are associated with adverse effects when used long-term. Therefore, the search for new drug candidates to treat IBD is imperative. The peroxisome proliferator-activated receptor-γ (PPARγ) is highly expressed in the colon. PPARγ plays a vital role in regulating colonic inflammation. 1,8-cineole, also known as eucalyptol, is a monoterpene oxide present in various aromatic plants which possess potent anti-inflammatory activity. Molecular docking and dynamics studies revealed that 1,8-cineole binds to PPARγ and if it were an agonist, that would explain the anti-inflammatory effects of 1,8-cineole. Therefore, we investigated the role of 1,8-cineole in colonic inflammation, using both in vivo and in vitro experimental approaches. Dextran sodium sulfate (DSS)-induced colitis was used as the in vivo model, and tumor necrosis factor-α (TNFα)-stimulated HT-29 cells as the in vitro model. 1,8-cineole treatment significantly decreased the inflammatory response in DSS-induced colitis mice. 1,8-cineole treatment also increased nuclear factor erythroid 2-related factor 2 (Nrf2) translocation into the nucleus to induce potent antioxidant effects. 1,8-cineole also increased colonic PPARγ protein expression. Similarly, 1,8-cineole decreased proinflammatory chemokine production and increased PPARγ protein expression in TNFα-stimulated HT-29 cells. 1,8-cineole also increased PPARγ promoter activity time-dependently. Because of its potent anti-inflammatory effects, 1,8-cineole may be valuable in treating IBD.
The Cytosolic Acetoacetyl-CoA Thiolase TaAACT1 Is Required for Defense against Fusarium pseudograminearum in Wheat
Authors: Xiong Feng , Zhu Xiuliang , Luo Changsha , Liu Zhixiang , Zhang Zengyan
Published: March 2023

Fusarium pseudograminearum is a major pathogen for the destructive disease Fusarium crown rot (FCR) of wheat (Triticum aestivum). The cytosolic Acetoacetyl-CoA thiolase II (AACT) is the first catalytic enzyme in the mevalonate pathway that biosynthesizes isoprenoids in plants. However, there has been no investigation of wheat cytosolic AACT genes in defense against pathogens including Fusarium pseudograminearum. Herein, we identified a cytosolic AACT-encoding gene from wheat, named TaAACT1, and demonstrated its positively regulatory role in the wheat defense response to F. pseudograminearum. One haplotype of TaAACT1 in analyzed wheat genotypes was associated with wheat resistance to FCR. The TaAACT1 transcript level was elevated after F. pseudograminearum infection, and was higher in FCR-resistant wheat genotypes than in susceptible wheat genotypes. Functional analysis indicated that knock down of TaAACT1 impaired resistance against F. pseudograminearum and reduced the expression of downstream defense genes in wheat. TaAACT1 protein was verified to localize in the cytosol of wheat cells. TaAACT1 and its modulated defense genes were rapidly responsive to exogenous jasmonate treatment. Collectively, TaAACT1 contributes to resistance to F. pseudograminearum through upregulating the expression of defense genes in wheat. This study sheds new light on the molecular mechanisms underlying wheat defense against FCR.
Over-Expression of LEDGF/p75 in HEp-2 Cells Enhances Autoimmune IgG Response in Patients with Benign Prostatic Hyperplasia—A Novel Diagnostic Approach with Therapeutic Consequence?
Authors: Liedtke Victoria , Rose Laura , Hiemann Rico , Nasser Abdullah , Rödiger Stefan , Bonaventura Alena , Winkler Laura , Sowa Mandy , Stöckle Michael , Schierack Peter , Junker Kerstin , Roggenbuck Dirk
Published: March 2023

Lens epithelium-derived growth factor splice variant of 75 kDa (LEDGF/p75) is an autoantigen over-expressed in solid tumors and acts as a stress-related transcriptional co-activator. Participation of autoimmune responses in the pathophysiology of benign prostatic hyperplasia (PBH) and a corresponding immunosuppressive therapy by TNFalpha antagonists has been recently suggested. Thus, autoAb testing could aid in the diagnosis of BPH patients profiting from such therapy. We generated CRISPR/Cas9 modified HEp-2 LEDGF knock-out (KO) and HEp-2 LEDGF/p75 over-expressing (OE) cells and examined IgG autoantibody reactivity to LEDGF/p75 in patients with prostate cancer (PCa, n = 89), bladder cancer (BCa, n = 116), benign prostatic hyperplasia (BPH, n = 103), and blood donors (BD, n = 60) by indirect immunofluorescence assay (IFA). Surprisingly, we could not detect elevated binding of autoAbs against LEDGF/p75 in cancer patients, but autoAb reactivity to LEDGF/p75 OE cells in about 50% of patients with BPH was unexpectedly significantly increased. Furthermore, a line immunoassay enabling the detection of 18 different autoAbs revealed a significantly increased occurrence of anti-dsDNA autoAbs in 34% of BPH patients in contrast to tumor patients and BD. This finding was confirmed by anti-mitochondrial (mDNA) autoAb detection with the Crithidia luciliae immunofluorescence test, which also showed a significantly higher prevalence (34%) of anti-mDNA autoAbs in BPH. In summary, our study provided further evidence for the occurrence of autoimmune responses in BPH. Furthermore, LEDGF/p75 over-expression renders HEp-2 cells more autoantigenic and an ideal target for autoAb analysis in BPH with a potential therapy consequence.
The Interplay between Integrins and Immune Cells as a Regulator in Cancer Immunology
Authors: Zhang Qingfang , Zhang Shuo , Chen Jianrui , Xie Zhenzhen
Published: March 2023

Integrins are a group of heterodimers consisting of α and β subunits that mediate a variety of physiological activities of immune cells, including cell migration, adhesion, proliferation, survival, and immunotolerance. Multiple types of integrins act differently on the same immune cells, while the same integrin may exert various effects on different immune cells. In the development of cancer, integrins are involved in the regulation of cancer cell proliferation, invasion, migration, and angiogenesis; conversely, integrins promote immune cell aggregation to mediate the elimination of tumors. The important roles of integrins in cancer progression have provided valuable clues for the diagnosis and targeted treatment of cancer. Furthermore, many integrin inhibitors have been investigated in clinical trials to explore effective regimens and reduce side effects. Due to the complexity of the mechanism of integrin-mediated cancer progression, challenges remain in the research and development of cancer immunotherapies (CITs). This review enumerates the effects of integrins on four types of immune cells and the potential mechanisms involved in the progression of cancer, which will provide ideas for more optimal CIT in the future.
Toxicological Potential of the FDA-Approved Treatment against Monkeypox. Comment on Zovi et al. Pharmacological Agents with Antiviral Activity against Monkeypox Infection. Int. J. Mol. Sci. 2022, 23, 15941
Authors: Morais Gabriel Christian de Farias , Fulco Umberto Laino , da Silva Edilson Dantas , Oliveira Claudio Bruno Silva de , Oliveira Jonas Ivan Nobre
Published: March 2023

Recently, some drugs were approved to control Monkeypox (MPX), among them tecovirimat. This was recently approved by regulatory agencies around the world, the paper of Zovi et al entitled Pharmacological Agents with Antiviral Activity against Monkeypox Infection highlight it as safe and effective, although the safety data are still not very robust. In this Comment, we present some theoretical evaluations of its safety, considering that for use in humans it is essential to have a rich scientific literature in the area. After a series of analyses, a potential risk of liver, respiratory and kidney damage was found in addition to carcinogenic potential. Thus, while we agree that there is a need for rapid responses to infection, we reinforce that well-designed and adequately powered studies should not only focus on investigating the pharmacological efficacy of tecovirimat but also demonstrate its safety in humans. Therefore, in this Comment, we present some concerns that may help in formulating a safer treatment for patients infected with Monkeypox virus (MPXV).
Synthesis, Characterization, and Antitumor Mechanism Investigation of Ruthenium(II)/Rhenium(I)-Daminozide Conjugates
Authors: Yang Pei-Xin , Xie Kai , Chen Mei-Ru , Zhang Zheng , Huang Bo , Li Rong-Tao , Ye Rui-Rong
Published: March 2023

Daminozide, a plant growth regulator, is an effective inhibitor of the Jumonji domain-containing protein (JMJD) histone demethylase. Herein, four ruthenium(II)/rhenium(I)-daminozide conjugates, with molecular formulas [Ru(N-N)2bpy(4-CH2OH-4′-CH2O-daminozide)](PF6)2 (Ru-1/Ru-2) (N-N = 1,10-phenanthroline (phen, in Ru-1) and 4,7-diphenyl-1,10-phenanthroline (DIP, in Ru-2)) and Re(N-N)(CO)3(PyCH2O-daminozide) (Re-1/Re-2) (Py = pyridine, N-N = phen (in Re-1) and DIP (in Re-2)), were synthesized and characterized. Among these complexes, Ru-2 and Re-2 exhibited higher cytotoxicity against tumor cells than cisplatin. Upregulation of H3K9Me3 expression level was found in human cervical cancer cells (HeLa) treated with Ru-2 and Re-2, indicating that these two complexes can inhibit the activity of JMJD histone demethylase. Further investigation revealed that Re-2 can selectively accumulate in the mitochondria of HeLa cells. Both Ru-2 and Re-2 can cause mitochondrial damage, induce apoptosis, and inhibit cell migration and colony formation of HeLa cells. Overall, these complexes exhibit multiple anticancer functions, including inhibiting JMJD, inducing apoptosis, and inhibiting cell invasion, making them promising candidates for anticancer drugs.
Modified Isoinertial-Based Ruffier Test in Healthy Individuals: A Feasibility Study
Authors: Trovato Bruno , Roggio Federico , Petrigna Luca , Musumeci Giuseppe
Published: March 2023

Cardiorespiratory fitness is an essential indicator in sports science and sports medicine that can be assessed with several tests. The Ruffier test is a submaximal test valid to estimate maximum oxygen uptake; it consists of 30 squats in 45 s, which may be challenging for inexperienced individuals. This study aims to verify the feasibility of a modified inertial-based version of the Ruffier test to assess cardiorespiratory fitness with 10 squats in 15 s. Both classic and isoinertial Ruffier tests were administered to thirty-five healthy young adults (20 men and 15 women), age 22.06 ± 2.13 years, BMI 23.87 ± 2.74. The two one-sided test confirmed the comparability of the isoinertial Ruffier test with its classic version within equivalence bounds of ±3.726. Furthermore, gender, age, body weight, the difference between peak heart rate after isoinertial squatting and resting heart rate, and the isoinertial Ruffier index are the coefficients of our best VO2max prediction model with an adjusted R2 of 0.937, sensitivity of 0.89, and specificity of 0.81. The study evidenced the feasibility of the isoinertial Ruffier test to measure cardiorespiratory fitness through a quick, safe, and short squat test easy to perform in fitness centers and primary care clinics.
Uncovering the Role of PdePrx12 Peroxidase in Enhancing Disease Resistance in Poplar Trees
Authors: Cai Guanghua , Zhang Yan , Huang Liyu , Wang Nian
Published: March 2023

Peroxidase (Prx)-related genes are reported to be involved in the metabolism of hydrogen peroxide (H2O2) in plants. Here, we found that the expression of the PdePrx12 gene was upregulated in wild-type (WT) poplar line NL895 infected with the pathogens Botryosphaeria dothidea strain 3C and Alternaria alternata strain 3E. The PdePrx12 gene was cloned in the poplar line NL895 and its overexpression (OE) and reduced-expression (RE) vectors were constructed. OE and RE transgenic lines were then generated. The H2O2 content in the leaves was measured by DAB staining and spectrophotometric analysis, and the data revealed that the OE line had a reduced H2O2 content, whereas the RE line had an increased H2O2 content. These transgenic and WT plants were also inoculated with the 3C/3E pathogens. The leaf area infected by pathogen 3C/3E was determined and the OE line was found to have a larger area of infection, whereas the RE line was found to have a smaller area of infection. This result suggested PdePRX12 is involved in disease resistance in poplar. Given these results, this study demonstrated that when poplar is infected by pathogens, the expression of PdePrx12 is inhibited, leading to an increase in H2O2 content, thereby enhancing disease resistance.
Scalar Measures of Volatility and Dependence for the Multivariate Models with Applications to Asian Financial Markets
Authors: Kim Sangwhan , Bera Anil K.
Published: March 2023

The variance–covariance matrix is a multi-dimensional array of numbers, containing information about the individual variabilities and the pairwise linear dependence of a set of variables. However, the matrix itself is difficult to represent in a concise way, particularly in the context of multivariate autoregressive conditional heteroskedastic models. The common practice is to report the plots of k(k−1)/2 time-varying pairwise conditional covariances, where k is the number of markets (or assets) considered; thus, when k=10, there will be 45 graphs. We suggest a scalar measure of overall variabilities (and dependences) by summarizing all the elements in a variance–covariance matrix into a single quantity. The determinant of the covariance matrix Σ, called the generalized variance, can be used as a measure of overall spread of the multivariate distribution. Similarly, the positive square root of the determinant |R| of the correlation matrix, called the scatter coefficient, will be a measure of linear independence among the random variables, while collective correlation+(1−|R|)1/2 will be an overall measure of linear dependence. In an empirical application to the six Asian market returns, these statistics perform the intended roles successfully. In addition, these are shown to be able to reveal and explain the empirical facts that cannot be uncovered by the traditional methods. In particular, we show that both the contagion and interdependence (among the national equity markets) are present and could be quantitatively measured in contrast to previous studies, which revealed only market interdependence.
Please Pass the Translanguaging: The Dinner Table Experience in the Lives of Newcomer Canadian Deaf Youth and Their Families
Authors: Weber Joanne Catherine , Jones Chelsea Temple , Atwal Abneet
Published: March 2023

While translanguaging occurs in the homes of deaf people and their hearing family members who do not sign or possess limited signing skills, in this article we argue that translanguaging alone does not explain the complex, domestic-sphere language experiences of three young, newcomer artists in Saskatchewan, Canada. We frame our inquiry around the “dinner table experience” phenomenon, wherein deaf family members receive partial or little access to conversational exchanges. At the dinner table, which is both a literal setting and a metaphor for exclusion experienced by deaf people in audiocentric cultures, many deaf family members report feeling loved yet disconnected. However, translanguaging serves to expand linguistic repertoires among hearing and deaf interlocutors amidst the dinner table experience. We draw from three interviews with deaf youth who describe the dinner table experience through both dialogue and art making, including descriptions of ways in which communication is facilitated or not facilitated, thereby highlighting available and unavailable translanguaging practices in the domestic sphere. The interview data suggest that the dinner table experience is a significant setting for translanguaging, and that promoting accessible and equitable translanguaging practices in the home remains a significant challenge, especially when combined with newcomer lived experience that does not always match current descriptions of translanguaging. We posit that translanguaging is a necessary practice among hearing and deaf persons at the table that can and should be expanded to consider the intersectional experiences of communicators in this literal and metaphorical setting.
Food Toxicity of Mycotoxin Citrinin and Molecular Mechanisms of Its Potential Toxicity Effects through the Implicated Targets Predicted by Computer-Aided Multidimensional Data Analysis
Authors: Zargar Seema , Wani Tanveer A.
Published: March 2023

The mycotoxin citrinin, which can contaminate food, is a major global concern. Citrinin is regarded as an inevitable pollutant in foods and feed since fungi are widely present in the environment. To identify contentious toxicity and lessen its severity by understanding the targets of citrinin in the human body and the impacted biosynthetic pathways, we analyzed the production of citrinin from Aspergillus flavus and Penicillium notatum and used a thorough bioinformatics analysis to characterize the toxicity and predict genes and protein targets for it. The predicted median fatal dosage (LD50) for citrinin was 105 mg/kg weight, and it belonged to toxicity class 3 (toxic if swallowed). Citrinin was found to be well absorbed by human intestinal epithelium and was a Pgp nonsubstrate (permeability glycoprotein), which means that once it is absorbed, it cannot be pumped out, hence leading to bioconcentration or biomagnification in the human body. The main targets of toxicity were casp3, TNF, IL10, IL1B, BAG3, CCNB1, CCNE1, and CDC25A, and the biological pathways implicated were signal transduction involved in DNA damage checkpoints, cellular and chemical responses to oxidative stress, DNA damage response signal transduction by P53, stress-activated protein kinase signaling cascade, netrin–UNC5B signaling, PTEN gene regulation, and immune response. Citrinin was linked to neutrophilia, squamous cell carcinoma, Fanconi anemia, leukemia, hepatoblastoma, and fatty liver diseases. The transcription factors E2F1, HSF1, SIRT1, RELA, NFKB, JUN, and MYC were found to be responsible. When data mining was performed on citrinin targets, the top five functional descriptions were a cell’s response to an organic cyclic compound, the netrin–UNC5B signaling pathway, lipids and atherosclerosis, thyroid cancer, and controlling the transcription of the PTEN gene.
Decreased Levels of Vitamin D in Bipolar Patients
Authors: Marazziti Donatella , Mangiapane Paola , Carbone Manuel Glauco , Morana Florinda , Arone Alessandro , Massa Lucia , Palermo Stefania , Violi Miriam , Bertini Giovanni , Massoni Leonardo , Fantasia Sara , Pozza Andrea , Mucci Federico , Morana Benedetto
Published: March 2023

Recently, vitamin D is considered a pleiotropic hormone, and as such, it has also become a topic of renewed interest in neuropsychiatry for its proposed role in the aetiology and pathophysiology of different psychiatric conditions, including mood disorders (MDs). This seems particularly crucial while considering the relatively high and often neglected prevalence of hypovitaminosis D in the general population and in specific groups, such as patients suffering from the most common type of MDs, which are major depression (MDD) and bipolar disorders (BDs). Therefore, in view of the controversial literature and findings on this topic and its potential therapeutic implications, the present study aimed at evaluating vitamin D levels in the plasma of a sample of inpatients fulfilling the DSM-5 criteria for mood episodes within BDs. The clinical picture was assessed by means of specific rating scales. The results showed that the vitamin D levels (mean ± SD, nM/L) of the bipolar patients of our sample were significantly lower (14.58 ± 11.27 nmol/L) than the normative values (>30 nmol/L). Eleven patients had sufficient values and only 4 had optimal, while 19 showed insufficient, 18 critical, and 17 severely critical levels. No differences emerged according to different socio-demographic or clinical features. In our opinion, the present findings strengthen previous research highlighting decreased vitamin D levels in bipolar patients and support the role of this pleiotropic hormone in BDs. Nevertheless, further studies should follow to corroborate the data of this preliminary study and to address the potential benefits of vitamin D supplementation in the treatment of MDs.
Renal Impairment Impact and Survival Analysis in a Romanian Cohort of HIV-1(F1)-Infected Children and Adolescents
Authors: Isac Raluca , Costa Rodica , Frandes Mirela , Lazureanu Voichita Elena , Stroescu Ramona Florina , Steflea Ruxandra Maria , Bagiu Iulia-Cristina , Horhat Florin George , Chicin Gratiana Nicoleta , Roberta Avram Cecilia , Cornelia Pacurari Alina , Doros Gabriela , Gafencu Mihai
Published: March 2023

Human immunodeficiency virus (HIV) is a lentivirus that is transmissible through blood and other body fluids. During the late 1980s and early 1990s, an estimated 10,000 Romanian children were infected with HIV-1 subtype F nosocomially through contaminated needles and untested blood transfusions. Romania was a special case in the global acquired immunodeficiency syndrome (AIDS) pandemic, displaying the largest population of HIV-infected children by parental transmission between 1987–1990. In total, 205 HIV-infected patients from the western part of Romania were analyzed in this retrospective study. Over 70% of them had experienced horizontal transmission from an unknown source, while vertical transmission was identified in only five cases. Most patients had a moderate to severe clinical manifestation of HIV infection, 77.56% had undergone antiretroviral (ARV) treatment, most of them (71.21%) had experienced no adverse reactions and many of those with HIV (90.73%) had an undetectable viral load. Renal impairment was detected in one third of patients (34.63%). Patients born before 1990, male patients, patients diagnosed with HIV before the age of 10, and those undernourished or with renal impairment had a shorter average survival time than the group born after 1990, female patients, patients receiving ARV treatment, patients with a normal body mass index (BMI) and those without renal impairment. Periodical monitoring of the estimated glomerular filtration rate (eGFR) level, as well as the detection of protein excretion, should be taken into consideration worldwide when monitoring HIV-positive patients; this in order to detect even asymptomatic chronic kidney disease (CKD), and to manage these patients and prolong their lives.
An Updated Review on Advances in Hydrogel-Based Nanoparticles for Liver Cancer Treatment
Authors: Farasati Far Bahareh , Isfahani Ali Attaripour , Nasiriyan Elnaz , Pourmolaei Ali , Mahmoudvand Golnaz , Karimi Rouzbahani Arian , Namiq Amin Mohammed , Naimi-Jamal Mohammad Reza
Published: March 2023

More than 90% of all liver malignancies are hepatocellular carcinomas (HCCs), for which chemotherapy and immunotherapy are the ideal therapeutic choices. Hepatocellular carcinoma is descended from other liver diseases, such as viral hepatitis, alcoholism, and metabolic syndrome. Normal cells and tissues may suffer damage from common forms of chemotherapy. In contrast to systemic chemotherapy, localized chemotherapy can reduce side effects by delivering a steady stream of chemotherapeutic drugs directly to the tumor site. This highlights the significance of controlled-release biodegradable hydrogels as drug delivery methods for chemotherapeutics. This review discusses using hydrogels as drug delivery systems for HCC and covers thermosensitive, pH-sensitive, photosensitive, dual-sensitive, and glutathione-responsive hydrogels. Compared to conventional systemic chemotherapy, hydrogel-based drug delivery methods are more effective in treating cancer.
Secondary Metabolites with Antifungal Activities from Mangrove Derived Fungus Monascus purpureus WMD2424
Authors: Wu Ming-Der , Chen Jih-Jung , Cheng Ming-Jen
Published: March 2023

The mold Monascus, also called red yeast rice, anka, or koji, has been used as the natural food coloring agent and food additives for more than 1000 years in Asian countries. It has also been used in Chinese herbology and traditional Chinese medicine due to its easing digestion and antiseptic effects. However, under different culture conditions, the ingredients in Monascus-fermented products may be changed. Therefore, an in-depth understanding of the ingredients, as well as the bioactivities of Monascus-derived natural products, is important. Here, through the thorough investigation into the chemical constituents of M. purpureus wmd2424, five previously undescribed compounds, monascuspurins A–E (1–5), were isolated from the EtOAc extract of mangrove-derived fungus Monascus purpureus wmd2424 cultured in RGY medium. All the constituents were confirmed via HRESIMS and 1D- and 2D-NMR spectroscopy. Their antifungal activity was also evaluated. Our results showed that four constituents (compounds 3–5) possessed mild antifungal activity against Aspergillus niger, Penicillium italicum, Candida albicans, and Saccharomyces cerevisiae. It is worth mentioning that the chemical composition of the type strain Monascus purpureus wmd2424 has never been studied.
Anatomical Augmentation Using Suture Tape for Acute Syndesmotic Injury in Maisonneuve Fracture: A Case Report
Authors: Yoon Sung-Joon , Jung Ki-Jin , Hong Yong-Cheol , Yeo Eui-Dong , Lee Hong-Seop , Won Sung-Hun , Lee Byung-Ryul , Ji Jae-Young , Lee Dhong-Won , Kim Woo-Jong
Published: March 2023

Ankle syndesmosis is crucial to the integrity of the ankle joint and weight-bearing; an injury to this structure can lead to significant disability. The treatment methods for distal syndesmosis injuries are controversial. The representative treatment methods include transsyndesmotic screw fixation and suture-button fixation, and good results with suture tape augmentation have recently been reported. However, an augmentation using suture tape is only possible when the posterior inferior tibiofibular ligament (PITFL) is intact. This study describes the case of an unstable syndesmosis injury, accompanied by anterior inferior tibiofibular ligament (AITFL) and PITFL injuries, which were treated successfully using suture tape. A 39-year-old male patient sustained right ankle damage while skateboarding. His leg and ankle radiographs revealed a widening of the medial clear space, a posterior malleolus fracture, a reduced “syndesmosis overlap” compared with the contralateral side, and a proximal fibula fracture. The magnetic resonance imaging revealed ruptured deltoid ligaments, accompanied by AITFL, PITFL, and interosseous ligament injuries. A diagnosis of a Maisonneuve fracture with an unstable syndesmotic injury was made. The patient underwent an open syndesmotic joint reduction, along with an AITFL and PITFL augmentation. This anatomical reduction was confirmed using intraoperative arthroscopy and postoperative computed tomography (CT). An axial CT that was performed at the 6-month follow-up exam revealed a similar alignment of the syndesmosis between the injured and uninjured sides. There were no surgical complications and the patient did not complain of discomfort in his daily life. At the 12-month follow-up exam, a good clinical outcome was confirmed. As a treatment for unstable syndesmosis injury, ligament augmentation using suture tape shows satisfactory clinical outcomes and can be considered as a useful and reliable method for anatomical restoration and rapid rehabilitation.
Prognostic Nutritional Index as a Novel Predictor of In-Stent Restenosis: A Retrospective Study
Authors: Balun Ahmet , Akgümüş Alkame , Özbek Kerem , Güven Çetin Zehra
Published: March 2023

Background and Objectives: In-stent restenosis (ISR) is a major problem in patients undergoing percutaneous coronary intervention. The prognostic nutritional index (PNI) is a nutritional status score used in the literature to determine the prognosis of coronary artery disease. In this study, we aimed to investigate the effect of preprocedural PNI values on the risk of ISR in patients with stable coronary artery disease who underwent successful percutaneous coronary intervention. Materials and Methods: This retrospective study included 809 patients. Stent restenosis was evaluated in the follow-up coronary angiography of the patients due to stable angina pectoris or acute coronary syndrome. The patients were divided into two groups based on those with (n = 236) and without (n = 573) in-stent restenosis, and their nutritional status was compared with PNI. The PNI values before the first angiography of the patients were calculated. Results: The mean PNI score was significantly lower in patients with ISR than in those without ISR (49.5 vs. 52.3, p < 0.001). Concerning the results of the Cox regression hazard model for predictors of ISR, PNI was significantly associated with the development of ISR (HR = 0.932, 95% CI: 0.909–0.956, p < 0.001). In addition, stent type, stent length, and diabetes mellitus were associated with the development of ISR. Conclusions: A low PNI value indicates poor nutritional status, which is thought to accelerate inflammation processes and cause atherosclerosis and ISR.
Short-Term Outcomes of Intravitreal Faricimab Injection for Diabetic Macular Edema
Authors: Kusuhara Sentaro , Kishimoto-Kishi Maya , Matsumiya Wataru , Miki Akiko , Imai Hisanori , Nakamura Makoto
Published: March 2023

Background and Objectives: Faricimab is a novel bispecific antibody with Fab regions inhibiting both vascular endothelial growth factor-A and angiopoietin-2. Therefore, this study aimed to obtain short-term outcomes of intravitreal injection of faricimab (IVF) for the treatment of diabetic macular edema (DME) in daily clinical practice. Materials and Methods: A retrospective review was carried out on consecutive patients with DME who had been treated with IVF and were followed up for at least 1 month. Outcome measures included changes in logMAR best-corrected visual acuity (logMAR BCVA), central retinal thickness (CRT), number of IVF administrations, and safety. Clinical outcomes were also compared between the treatment-naïve and switch groups. Results: A total of 21 consecutive DME eyes from 19 patients were identified. The mean number of IVFs was 1.6 ± 0.8 during the mean follow-up time of 5.5 months. The overall mean logMAR BCVA following IVF was 0.236, 0.204, 0.190, and 0.224 at baseline, 1, 3, and 6 months, respectively, without a significant change from baseline to 1 month (p = 0.176) or for 6 months (p = 0.923). The overall mean CRT (μm) following IVF was 400.6, 346.6, 342.1, and 327.5 at baseline, 1, 3, and 6 months, respectively. CRT significantly decreased from baseline to 1 month (p = 0.001) but did not reach a significant level over 6 months following IVF (p = 0.070). No significant difference in BCVA or CRT was observed between the treatment-naïve and switch groups. No serious safety concerns were noted. Conclusions: IVF for the treatment of DME may preserve visual acuity and improve macular thickness without serious safety concerns in the short term in a real-world clinical setting.
A Mathematical Simulation of Copper and Nickel Ions Separation Using Prepared Nanocellulose Material
Authors: Aljlil Saad
Published: March 2023

Environmental risks can arise from the existence of heavy metals in wastewater and their land disposal. To address this concern, a mathematical technique is introduced in this article that enables the anticipation of breakthrough curves and the imitation of copper and nickel ion separation onto nanocellulose in a fixed-bed system. The mathematical model is based on mass balances for copper and nickel and partial differential equations for pore diffusion in a fixed bed. The study evaluates the impact of experimental parameters such as bed height and initial concentration on the shape of the breakthrough curves. At 20 °C, the maximum adsorption capacities for copper and nickel ions on nanocellulose were 5.7 mg/g and 5 mg/g, respectively. The breakthrough point decreased with increasing solution concentration at higher bed heights, while at an initial concentration of 20 mg/L, the breakthrough point increased with bed height. The fixed-bed pore diffusion model showed excellent agreement with the experimental data. The use of this mathematical approach can help alleviate the environmental hazards that arise from the presence of heavy metals in wastewater. The study highlights the potential of nanocellulose as a material for membrane technology, which can effectively address these risks.
Editorial for the Special Issue “Microsystem for Electronic Devices”
Authors: Ding Xinrui
Published: March 2023

The field of microsystems is a rapidly evolving area with a wide range of applications in the field of electronics [...]
Development and In Vitro Characterization of Antibiotic-Loaded Nanocarriers for Dental Delivery
Authors: Ghazwani Mohammed , Vasudevan Rajalakshimi , Kandasamy Geetha , Hani Umme , Niharika Gaddam , Naredla Manusri , Devanandan Praveen , Puvvada Ranadheer Chowdary , Almehizia Abdulrahman A. , Hakami Abdulrahim R. , Dhurke Rajeshri
Published: March 2023

The aim of this research work was to formulate and evaluate ciprofloxacin hydrochloride-loaded nanocarriers for treating dental infections and bone regeneration. Periodontal infection is associated with inflammation, soft tissue destruction, and bone loss. The objective of the study was to extract β tricalcium phosphate (β-TCP) from coral beach sand using the hydrothermal conversion method and load these nanocarriers with ciprofloxacin hydrochloride. The developed drug-loaded nanocarriers were evaluated for various parameters. In vitro drug-loading studies showed the highest drug loading of 71% for F1 with a drug: carrier ratio compared to plain ciprofloxacin hydrochloride gel. β-TCP and nanocarriers were evaluated for powder characteristics and the results were found to have excellent and fair flowability. In vitro drug release studies conducted over a period of 5 days confirmed the percentage drug release of 96% at the end of 120 h. Nanocarriers were found to be effective against S. aureus and E. coli showing statistically significant antibacterial activity at (* p < 0.05) significant level as compared to plain ciprofloxacin hydrochloride gel. The particle size of β-TCP and nanocarriers was found to be 2 µm. Fourier transform infra-red studies showed good compatibility between the drug and the excipients. Differential scanning calorimetry studies revealed the amorphous nature of the nanocarriers as evident from the peak shift. It is obvious from the XRD studies that the phase intensity was reduced, which demonstrates a decrease in crystallinity. Nanocarriers released the drug in a controlled manner, hence may prove to be a better option to treat dental caries as compared to conventional treatments.
Nanoshaped Cerium Oxide with Nickel as a Non-Noble Metal Catalyst for CO2 Thermochemical Reactions
Authors: Serafin Jarosław , Llorca Jordi
Published: March 2023

Four different nanoshapes of cerium dioxide have been prepared (polycrystals, rods, cubes, and octahedra) and have been decorated with different metals (Ru, Pd, Au, Pt, Cu, and Ni) by incipient wetness impregnation (IWI) and ball milling (BM) methods. After an initial analysis based on oxygen consumption from CO2 pulse chemisorption, Ni-like metal, and two forms of CeO2 cubes and rods were selected for further research. Catalysts were characterized using the Brunauer-Emmett-Teller formula (BET), X-ray spectroscopy (XRD), Raman spectroscopy, scanning electron microscopy (SEM), UV–visible spectrophotometry (UV-Vis), X-ray photoelectron spectroscopy (XPS), temperature programmed reduction (H2-TPR) and CO2 pulse chemisorption, and used to reduce of CO2 into CO (CO2 splitting). Adding metals to cerium dioxide enhanced the ability of CeO2 to release oxygen and concomitant reactivity toward the reduction of CO2. The effect of the metal precursor and concentration were evaluated. The highest CO2 splitting value was achieved for 2% Ni/CeO2-rods prepared by ball milling using Ni nitrate (412 µmol/gcat) and the H2 consumption (453.2 µmol/gcat) confirms the good redox ability of this catalyst.
Blue, Yellow, and Red Carbon Dots from Aromatic Precursors for Light-Emitting Diodes
Authors: Liu Zhenzhen , Lu Xiaofei , Liu Menglin , Wang Wenjing
Published: March 2023

In this work, multicolor fluorescent carbon dots with red (R-CDs), yellow (Y-CDs), and blue (B-CDs) emissions were prepared by choosing proper aromatic precursors with different amounts of benzene rings through a simple solvothermal method. The characterization showed that the prepared carbon dots were spherical with a size under 10 nm, rich surface functional groups, and good stability. The emission wavelengths were located at 440, 530, and 580 nm under the excitation of 370 nm. The relative fluorescence quantum yield (QY) of R-CDs, Y-CDs, and B-CDs was 11%, 59%, and 33%, respectively. The related characterization demonstrated that the redshift in the photoluminescence was caused by the synergistic effect of the increasing graphitic nitrogen content, quantum size effect and surface oxidation state. By mixing the three prepared CDs into a PVA matrix, the transparent and flexible films produced relucent blue, yellow, and red emissions under 365 nm UV light, and solid-state quenching was effectively avoided. LEDs were fabricated by using B-CDs, Y-CDs, and R-CDs/PVA with a semiconductor chip. These CDs-based LEDs produced bright blue, yellow, and red light with CIE color coordinates of (0.16, 0.02), (0.38, 0.58), and (0.50, 0.49) were successfully manufactured utilizing the prepared blue, yellow and red multicolor carbon dots as the solid luminescent materials. The results showed that the synthesized CDs can be potentially applied in multi-color monitors as a promising candidate for light-emitting diodes (LEDs). This work blazes a novel trail for the controllable preparation of multicolor fluorescent carbon dots.
Modeling “Stag and Hare Hunting” Behaviors Using Interaction Data from an mCSCL Application for Grade 5 Mathematics
Authors: Bringula Rex P. , Enverzo Ann Joizelle D. , Gonzales Ma. Gracia G. , Rodrigo Maria Mercedes T.
Published: March 2023

This study attempted to model the stag and hare hunting behaviors of students using their interaction data in a mobile computer-supported collaborative learning application for Grade 5 mathematics. Twenty-five male and 12 female Grade 5 students with an average age of 10.5 years participated in this study. Stag hunters are more likely to display personality dimensions characterized by Openness while students belonging to hare hunters display personality dimensions characterized by Extraversion and Neuroticism. Students who display personality dimensions characterized by Agreeableness and Conscientiousness may tend to be either hare or stag hunters, depending on the difficulty, types of arithmetic problems solved, and the amount of time spent solving arithmetic problems. Students engaged in a stag hunting behavior performed poorly in mathematics. Decision tree modeling and lag sequential analysis revealed that stag and hare hunting behaviors could be identified based on personality dimensions, types of arithmetic problems solved, difficulty level of problems solved, time spent solving problems, and problem-solving patterns. Future research and practical implications were also discussed.
The HIE-FDTD Method for Simulating Dispersion Media Represented by Drude, Debye, and Lorentz Models
Authors: Chen Juan , Mou Chunhui
Published: March 2023

The hybrid implicit–explicit finite-difference time-domain (HIE-FDTD) method is a weakly conditionally stable finite-difference time-domain (FDTD) method that has attracted much attention in recent years. However due to the dispersion media such as water, soil, plasma, biological tissue, optical materials, etc., the application of the HIE-FDTD method is still relatively limited. Therefore, in this paper, the HIE-FDTD method was extended to solve typical dispersion media by combining the Drude, Debye, and Lorentz models with hybrid implicit–explicit difference techniques. The advantage of the presented method is that it only needs to solve a set of equations, and then different dispersion media including water, soil, plasma, biological tissue, and optical materials can be analyzed. The convolutional perfectly matched layer (CPML) boundary condition was introduced to truncate the computational domain. Numerical examples were used to validate the absorbing performance of the CPML boundary and prove the accuracy and computational efficiency of the dispersion HIE-FDTD method proposed in this paper. The simulated results showed that the dispersion HIE-FDTD method could not only obtain accurate calculation results, but also had a much higher computational efficiency than the finite-difference time-domain (FDTD) method.