bluuebunny/crossref_metadata_2025 huggingface dataset:
The Institutionalization of Implicit and Explicit CSR in a Developing Country Context: The Case of Lebanon
Authors: Kobrossy Samer , Karaszewski Robert , AlChami Riad
Published: October 2022

This article presents a qualitative study pertaining to the interface between business and society that primarily focuses on the prominent institutional factors that are influencing organizations to adopt explicit and implicit forms of corporate social responsibility in a developing country context. This is a pertinent subject because developing countries contain a large proportion of the world’s population and, subsequently, a large proportion of salient issues in the social, economic, and environmental arena. This study identifies that, in the country context chosen namely Lebanon, explicit social responsibility initiatives are driven by predominantly normative and mimetic institutional pressures emanating from both local and international institutions. This study also identifies that implicit social responsibility initiatives are driven by normative institutional pressures emanating from elements in the national business system. Contributions of this study include a conceptual model to aid future research, identifying the importance of the duality of corporate social responsibility, the importance of international institutions in facilitating explicit social responsibility proliferation, and the importance for managers to include explicit social responsibility initiatives in their organizational mandate. Future research can use a different sampling technique, conduct interviews with employees that are not directly involved with social responsibility initiatives, or use other perspectives, such as agency theory or theory of institutional logics, to gain more insight on the topic.
Codling Moth Monitoring with Camera-Equipped Automated Traps: A Review
Authors: Suto Jozsef
Published: October 2022

The codling moth (Cydia pomonella) is probably the most harmful pest in apple and pear orchards. The crop loss due to the high harmfulness of the insect can be extremely expensive; therefore, sophisticated pest management is necessary to protect the crop. The conventional monitoring approach for insect swarming has been based on traps that are periodically checked by human operators. However, this workflow can be automatized. To achieve this goal, a dedicated image capture device and an accurate insect counter algorithm are necessary which make online insect swarm prediction possible. From the hardware side, more camera-equipped embedded systems have been designed to remotely capture and upload pest trap images. From the software side, with the aid of machine vision and machine learning methods, traditional (manual) identification and counting can be solved by algorithm. With the appropriate combination of the hardware and software components, spraying can be accurately scheduled, and the crop-defending cost will be significantly reduced. Although automatic traps have been developed for more pest species and there are a large number of papers which investigate insect detection, a limited number of articles focus on the C. pomonella. The aim of this paper is to review the state of the art of C. pomonella monitoring with camera-equipped traps. The paper presents the advantages and disadvantages of automated traps’ hardware and software components and examines their practical applicability.
Wheat Crop Yield and Changes in Soil Biological and Heavy Metals Status in a Sandy Soil Amended with Biochar and Irrigated with Drainage Water
Authors: Abd El-Azeim Mohieyeddin M. , Menesi Ahmad M. , Abd El-Mageed Mahmoud M. , Lemanowicz Joanna , Haddad Samir A.
Published: October 2022

The current research aims to study the impacts of adding corncob biochar to a sandy soil irrigated with drainage water on wheat productivity, heavy metals fate, and some soil properties that reflect healthy soil conditions. This research consists of two separate experiments under field (lysimeters) and pot incubation conditions conducted on sandy soil irrigated with drainage water and treated with corncob biochar at the rate of 0.0, 1, 2, and 3% as mixing or mulching. Results specified that drainage water electrical conductivity value (5.89 dS m−1) lies under the degree of restriction on use of “Severe”, indicating that nonstop irrigation with such drainage water may cause a severe salinity problem in soil in the long run. A comparison of heavy metal concentrations of biochar-treated soils with the control showed that total heavy metals had accumulated significantly in the topsoil layer. Most of the available heavy metal concentrations in all soil leachate fractions were below the method detection limits. Mean concentrations of Ni, Cd, and Pb in wheat crops were far below the concentrations considered phytotoxic to wheat plants. More than 90% of the Ni, Cd, and Pb contained in the drainage water of the Al-Moheet drain were significantly present (p ≤ 0.05) and adsorbed by biochar in the top 20 cm of soil lysimeters, indicating the high biochar adsorptive capacity of heavy metals. Total counts of bacteria and fungi gradually and significantly increased over the soil incubation time despite irrigation with contaminated drainage water. Soil resistance index (SRI) values for microbial biomass were positive throughout the experiment and increased significantly as the application rate of corncob biochar increased. These results indicated the high feasibility of using corncob biochar at a rate of 3% to temporarily improve the health of sandy soil despite irrigation with drainage water.
Effects of Varieties, Cultivation Methods, and Origins of Citrus sinensis ‘hongjiang’ on Volatile Organic Compounds: HS-SPME-GC/MS Analysis Coupled with OPLS-DA
Authors: Huang Xiangwu , Zhao Lihong , Pang Sheng , Liu Yijun , Liu Jianrong , Zhang Meiqian
Published: October 2022

Volatile organic compounds (VOCs) in Citrus sinensis ‘hongjiang’ oranges significantly vary depending on the fruit variety, cultivation mode, and cultivation location. The effect of these three factors on VOCs was experimentally determined in this study. In total, 102 VOCs were separated via headspace solid-phase microextraction and identified via gas chromatography-mass spectrometry, and the differential components were analyzed by partial least-squares-discriminant analysis (OPLS-DA). The VOCs of ‘hongjiang’ mainly comprised alkenes, alcohols, aldehydes, and ketones. They were well clustered in OPLS-DA and principal component analysis (PCA), and the seven groups were distinctly differentiated. The results of the S-plot, variable importance in projection (VIP), and heatmap analyses showed that these factors had a significant impact on VOCs in ‘hongjiang’. The characteristic VOCs between the two pairs were significant, while the net room cultivation mode had the most substantial effect on VOCs.
Environmental and Energy Analysis of Two Orchard Systems: A Case Study in Mediterranean Environment
Authors: Pergola Maria , Persiani Alessandro , D’Ammaro Daniele , Pastore Vittoria , D’Adamo Carmine , Palese Assunta Maria , Celano Giuseppe
Published: October 2022

This paper aimed to analyse and compare the environmental and energy sustainability of two orchards (peach versus kiwifruit) located in Southern Italy using Life Cycle Thinking. To this end, anthropogenic energy, CO2 emissions, biogenic energy and carbon gains were also considered through Life Cycle Assessment methodology and Energy Analysis. The C–CO2 balance was calculated as the difference between total C–CO2 stored in soil and trees, at the end of their life cycles, and orchards Carbon Footprint (CF). The results showed that the production of 1 kg of peaches caused minor impacts, especially with reference to CF (0.124 kg CO2 eq against 0.145 for kiwifruit), while it required 1.56 MJ of energy against 1.32 MJ for kiwifruit. In both orchards the main sources of direct CO2 emissions came from fuel combustion, nitrous oxide release by crop residue decomposition, and nitrogenous fertilizer distribution. Nevertheless, both orchards had sustainable environmental and energy results. Despite the management of the orchards releasing CO2 and consuming energy, they showed a significant capacity to store CO2 and energy, proving to be virtuous systems. This research can give useful indications for farmers, farmer associations, technicians, and stakeholders to improve orchard management efficiency. The net balance approach seems to be an adequate strategy, allowing best estimation of environmental impacts and guiding farmer decisions towards more sustainable alternatives.
Spatial Contribution of Environmental Factors to Soil Aggregate Stability in a Small Catchment of the Loess Plateau, China
Authors: Ye Luping , Ji Lingling , Chen Hongfeng , Chen Xueye , Tan Wenfeng
Published: October 2022

Soil aggregate stability and erodibility are the influential factors governing soil resistance to water erosion. The interactions among aggregate stability, erodibility, and their influencing factors have not been fully explored. We collected soil samples from 0–10 cm and 10–20 cm layers in the Zhifanggou watershed. Then, the major contributors to aggregate stability and erodibility and how soil properties, environmental factors and land use contributed to them were explored by using partial least-squares regression and path analysis, respectively. The results showed that the major contributors included the slope, soil organic carbon (SOC), elevation, the percentage of landscape area of farmland (PLAND_F) and grassland (PLAND_G), the land surface temperature difference between seasons (ΔLST), topographic wetness index (TWI), pH, amorphous iron (poorly ordered forms of iron, Feo), and calcium carbonate (CaCO3). In which, the slope, SOC, and elevation were the most important contributors to the mean weight diameter (MWD) and the percentage of water-stable aggregates greater than 0.25 mm (WSA>0.25) and had a direct contribution to MWD, WSA>0.25, and K factors. The PLAND_F and PLAND_G had a significant and indirect contribution to those three indices by affecting slope. Meanwhile, the effects of pH, Feo, and CaCO3 on WSA>0.25 should also not be underestimated. For MWD and WSA>0.25, there was a significantly higher effect of the land use types and composition than hydrothermal conditions. For K factors, PLAND’s contribution was still higher than ΔLST and TWI, but they were all significant. The other soil properties, including pH, CaCO3, and Feo, indirectly affected them by influencing SOC. However, the direct contributions of soil properties increased as the soil layer deepened.
Research on the High-Quality Development Path of the Cross-Border Agricultural Product Supply Chain between China and Central Asia
Authors: Abula Kahaer , Abula Buwajian , Hu Qingjiang , Chen Xinyu , Wang Dezhen
Published: October 2022

As a bridge and link between China and Central Asian countries, logistics infrastructure plays an important role in the process of forming a new development pattern of “dual circulation” and mutual promotion. Based on the panel data of Central Asian countries from 2010 to 2019, this paper uses random effect model to analyze the impact on the cross-border agricultural product supply chain between China and Central Asian countries. The results show that the logistics development level, railway transport connectivity, road transport connectivity, economic development level, market size, and common border of Central Asian countries positively affect the development level of the supply chain at different significance levels. Among them, the degree of railway transport connectivity has the greatest impact on the supply chain, followed by the common border, and then the level of logistics development. Moreover, the geographical advantage cannot be underestimated and it plays a positive role in promoting the high-quality development of cross-border agricultural product supply chain. China borders Kazakhstan, Kyrgyzstan, and Tajikistan, and the agricultural product import and export trade quota is significantly greater than that of other Central Asian countries. Based on the analysis, we propose to strengthen efforts to build a high-quality logistics system, build an efficient land transport network, and break through cross-border logistics congestion and difficulties.
A New Approach on Making European Agriculture More Efficient under Uncertainty Conditions
Authors: Ionescu Romeo Victor , Zlati Monica Laura , Antohi Valentin Marian
Published: October 2022

Agriculture is a strategic sector of the European economy in the current economic, social, climatic, and geo-political conditions generated by global crisis and the war in Ukraine. The main objective of the research is to quantify the vulnerabilities of EU agricultural evolution and to assess the opportunities for development through the building of a scoreboard of viable agricultural development solutions in line with the needs expressed in the current unfavourable context. The importance of this research is related to smart agriculture as a solution to the food crisis generated by the same uncertainty conditions. The methods used are empirical literature review and econometric modelling of vulnerabilities based on the dynamic evolution of branch efficiency and effectiveness indicators under exogenous events (economic crisis, geo-political crisis, soil and climate crisis, health crisis), collected from official data sources. The outcome of the study is the identification of viable, implementable solutions to ensure the planned success of the sustainable development of the branch.
Comparative Analysis of Pedigree-Based BLUP and Phenotypic Mass Selection for Developing Elite Inbred Lines, Based on Field and Simulated Data
Authors: Viana José Marcelo Soriano , Dias Kaio Olimpio das Graças , Silva Jean Paulo Aparecido da
Published: October 2022

Pedigree-based best linear unbiased prediction (pBLUP) is an effective method for genetic evaluation. The objective of this study was to assess the efficacy of pBLUP to develop superior inbred lines, using field and simulated data. This study was based on the data of 30,986 plants from six S0 generations and eight inbred progeny trials (S1–S4), performed over eight years. We measured the grain yield and the expansion volume (EV). We also simulated the EV and grain yield of 5000 plants (S0–S4) in two in silico populations, assuming 300 and 400 genes distributed across 10 chromosomes of 200 cM, respectively, and 10% of pleiotropic genes. We selected selfed plants based on the predicted additive value for EV by fitting the individual model in inbred population. The use of pBLUP provided total genetic gains in EV in the range of 1 to 45%, inversely proportional to the level of improvement of the population, and indirect changes in grain yield in the range of −17 to 3%. Only from the analysis of the in silico populations, assuming selection based on the true additive value and genetic gain computed from the genotypic values, there was evidence that pBLUP is superior to phenotypic mass selection.
Effects of Spraying Calcium Fertilizer on Photosynthesis, Mineral Content, Sugar–Acid Metabolism and Fruit Quality of Fuji Apples
Authors: Wang Guiping , Wang Jinzheng , Han Xueping , Chen Ru , Xue Xiaomin
Published: October 2022

This study was conducted at the Taidong Base of the Shandong Institute of Pomology, Tai’an City, Shandong Province, China, from May to October 2020. The effects of spraying calcium fertilizer on the photosynthetic characteristics, mineral element content, sugar and acid metabolism, and quality of apples were assessed. Fuji Apple Tianhong.2/SH/Malus robusta (M. robusta) was treated with the calcium fertilizers Niucui (Ca ≥ 100 g/L, B: 1–10 g/L) and Naipu 9 (Ca: 90 g/L, Mg: 21 g/L, N: 110 g/L) at the young fruit stage (25 May), the expansion stage (5 July), and the coloring stage (25 September). Water was sprayed as the control application. The results revealed that spraying calcium fertilizer increased the chloroplast pigment content and photosynthetic capacity of the apple leaves. Compared with the control, the net photosynthetic rate of apple leaves sprayed with Niucui increased by 4.3–34.6%, and that of leaves sprayed with Naipu 9 increased by 15.0–57.4%. Spraying calcium fertilizer promoted the accumulation of Ca, Mg, and B mineral elements in leaves and fruits while inhibiting the accumulation of Cu. Spraying calcium fertilizer improved the quality of apple fruit. Compared to the control fruit, the single fruit weight, fruit hardness, soluble solid content, and Vitamin C content of fruit sprayed with Niucui and Naipu 9 increased by 6.5 and 12.1%, 3.6 and 16.1%, 6.3 and 12.0%, and 30.5 and 29.4%, respectively. Spraying calcium fertilizer increased the sugar content and decreased the acid content of the apples. Naipu 9 was more effective at increasing fruit sugar content, especially sucrose and sorbitol, while Niucui was more effective at reducing the acid content, especially malic acid. This was observed because Naipu 9 increased the sugar metabolism enzyme activity, while Niucui increased the acid decomposition-related enzyme activity. In conclusion, according to the nutrition quality and flavor of the fruit, Naipu 9 application was determined to be the best fertilizer for the Fuji apple.
Studies on the Removal of Phenol and Nitrophenols from Water by Activated Carbon Developed from Demineralized Kraft Lignin
Authors: Chaudhary Monika , Suhas  , Kushwaha Sarita , Chaudhary Shubham , Tyagi Inderjeet , Dehghani Mohammad Hadi , Stephen Inbaraj Baskaran , Goscianska Joanna , Sharma Minaxi
Published: October 2022

The present investigation deals with the importance of interaction and position of the nitro group in the adsorptive removal of 2-nitrophenol (2-NP), 4-nitrophenol (4-NP) and phenol by demineralized kraft lignin activated carbon (DKLAAC). The adsorption of phenol and NPs on DKLAAC was found to follow the order 2-NP > 4-NP > phenol. In this study, both solubility and donor–acceptor complex mechanism played an important role besides the porosity and surface area of the materials. Accordingly, the NP possessing the least solubility would noticeably exhibit a higher affinity to be adsorbed at the solid–liquid interface. Thus the highly hydrophobic 2-NP was adsorbed to a greater extent followed by 4-NP and phenol. Moreover, the adsorption capacity as affected by contact time, initial phenol concentration, pH, and temperature was also investigated. The experimental adsorption capacity by DKLAAC was 2.09, 2.34, and 2.20 mmol·g−1 for phenol, 2-NP, and 4-NP at 25 °C, respectively, with the maximum amount being adsorbed within 40 min. The experimental data obtained for the removal of phenol and NPs were adequately fitted by the Langmuir adsorption isotherm and pseudo-second order kinetic models. Additionally, the temperature study revealed the adsorption process to be endothermic and spontaneous with high affinity between DKLAAC and phenols.
Effect of Different Long-Term Potassium Dosages on Crop Yield and Potassium Use Efficiency in the Maize–Wheat Rotation System
Authors: He Bing , Xue Cheng , Sun Zhimei , Ji Qingkai , Wei Jing , Ma Wenqi
Published: October 2022

Potassium (K) is the second most important plant nutritional element and is used for numerous physiological processes. We established an eight-year experiment comparing the effects of five K fertilization treatments (0, 48, 84, 120 and 156 kg K ha−1) on crop yield, K use efficiency and soil apparent K balance under the maize–wheat rotation system in the North China Plain. The highest maize and wheat yields were achieved in the K120 treatment, increasing by up to 16.7% and 25.1%, respectively. The increase in grain yield and K agronomic efficiency (AEK) with K application was greater in wheat than in maize. The K recovery efficiency (REK) and K accumulative recovery efficiency (ARE) significantly decreased with the increase in K fertilization in the maize and wheat seasons. However, the soil apparent K balance and soil available K content increased; the former was deficiency (−24.3 kg ha−1 yr−1) in the K0 treatment, but the latter did not decrease significantly compared with that in the initial year of the experiment. The soil available K content increased by 10.9 mg kg−1 per 100 kg ha−1. In conclusion, the yield response to K fertilization was greater in wheat than in maize and alleviated soil K depletion, but the K fertilizer efficiency was lower. We believed that K fertilizer can be increased moderately in the wheat season and decreased in the maize season.
Temporal Analysis of Candidatus Liberibacter asiaticus in Citrandarin Genotypes Indicates Unstable Infection
Authors: Cavichioli Thais Magni , Curtolo Maiara , Cristofani-Yaly Mariangela , Rodrigues Josiane , Coletta-Filho Helvécio Della
Published: October 2022

Background: Huanglongbing (HLB) is currently one of the most devasting diseases in citrus plants worldwide. Resistance against its causal agent, Candidatus Liberibacter asiaticus (CLas), in commercial Citrus species remains a challenge, even though they show differences in CLas multiplication. Methods: A total of 14 citrandarins and their parents (Sunki mandarin and Poncirus trifoliata cv. Rubidoux) were top-grafted onto the canopy of potted ‘Valencia’ sweet orange plants with high CLas titers. The grafted genotypes were tested for CLas infection and physiological effects of the disease (starch accumulation and callose deposition) for 12 months. Results: All tested genotypes were infected by CLas during the time frame of the experiment. However, a decrease in the infection rate ranging from 50% to 80% for the hybrids H109, H126, H157, and H222 was observed 360 days from the top-grafting. CLas was undetected by real-time PCR in H106 at the end of the experiment, which had low levels of starch and callose deposition. Conclusions: CLas infected all of the tested citrandarins, but a decrease in the infection rate over time was detected for some specific genotypes, which led to less starch accumulation and callose deposition.
A Novel Locus for Bakanae Disease Resistance, qBK4T, Identified in Rice
Authors: Lee Sais-Beul , Lee Ji-Yoon , Kang Ju-Won , Mang Hyunggon , Kabange Nkulu Rolly , Seong Gi-Un , Kwon Youngho , Lee So-Myeong , Shin Dongjin , Lee Jong-Hee , Cho Jun-Hyeon , Oh Ki-Won , Park Dong-Soo
Published: October 2022

Bakanae disease caused by Fusarium fujikuroi causes crop failure and yield losses in the majority of rice-growing countries. In this study, we employed a joint strategy quantitative trait locus (QTL) mapping–Genome-Wide Association Study (GWAS) to investigate novel genetic loci associated with Bakanae disease resistance using a population of 143 BC1F8 RILs derived from a cross between Ilpum × Tung Tin Wan Hien1. The phenotypic data from the bioassay and the genotypic data generated using a DNA chip were utilized to perform QTL mapping and GWAS study. Our results identified a novel genetic locus qBK4T associated with Bakanae disease resistance, which was mapped on chromosome 4 and flanked by AX-116847364 (33.12 Mbp) and AX-115752415 (33.44 Mbp) markers covering a region of 324kbp. There were 34 genes in this region including Os04g55920 (encoding a zinc-finger protein, OsJAZ1), Os04g55970 (encoding AP2-like ethylene-responsive transcription factor), etc. This study proposes qBK4T as a novel locus for Bakanae disease resistance. The identification of qBK4T and its flanking marker information could be useful for marker-assisted breeding and functional characterization of resistance genes against bakanae disease.
Phosphorus Fractions in Temperate Grassland Soils and Their Interactions with Agronomic P Tests
Authors: Graça Jessica , Bondi Giulia , Schmalenberger Achim , Daly Karen
Published: October 2022

Phosphorus (P) is a major nutrient for plant growth and animal health, and its availability in soil is influenced by agronomic management and soil properties. This study examined the distribution of labile and non-labile soil P fractions in grassland and the properties that influence their distribution in temperate grasslands. Eleven fractions were identified as, labile (H2O.P and NaHCO3.P), moderately labile (NaOH.P) and plant non-available (NaOH.P, HCl.P and residual P). The residual P, moderately labile organic (NaOH.Po) and inorganic (NaOH.Pi) fractions comprised 38%, 24% and 15% of the total P (TP), respectively. The residual and organic (Po) fractions were associated with extractable Fe, and the NaOH.Pi fraction was associated with extractable Al. Agronomic reagents (Morgan’s and Mehlich3) targeted specific fractions based on their affinity for different soil elements. Morgan’s P was associated with extractable and residual Ca and in high Ca soils, and overestimated plant-available P by extracting non-labile Ca.P forms (HCl.P). The Mehlich3 P test was associated with NaOH.Pi fraction and showed a preference for Al.P oxides. This study indicates that Mehlich3 reagent, which provides a wider suite of elements such as P, Ca, Al and Fe, will suit as a better indicator for soil P dynamics and improved P advice to farmers. The inclusion of additional soil chemico-physical characteristics would improve agronomic soil testing, benefitting farmers’ advice.
Winter Cereal Reactions to Common Root Rot and Crown Rot Pathogens in the Field
Authors: Saad Ahmed , Macdonald Bethany , Martin Anke , Knight Noel L. , Percy Cassandra
Published: October 2022

In Australia, Fusarium pseudograminearum and F. culmorum are the two main fungi causing crown rot, while Bipolaris sorokiniana is the causal agent of common root rot. Fusarium graminearum is typically linked with Fusarium head blight; however, it has been associated with crown rot in Australia and other parts of the world. This study investigated the reactions of single cultivars of barley, bread wheat, durum wheat, oat, and triticale to inoculation with strains of F. pseudograminearum, F. culmorum, F. graminearum and B. sorokiniana in field trials across two seasons. Fusarium pseudograminearum and F. culmorum caused greater visual discolouration than F. graminearum and B. sorokiniana on both stems and sub crown internodes of all hosts. Fusarum pseudograminearum caused the greatest reduction in plant dry weight across hosts in both years. Durum wheat (cv. Hyperno) barley (cv. Grimmett), bread wheat (cv. Livingston) and triticale (cv. Endeavour) observed significantly high levels of visual discolouration on stems when inoculated with F. pseudograminearum, while oat (cv. Genie) exhibited the least visual discolouration. Despite variation in the visual discolouration, the DNA of all pathogens were detected in all cultivars. This research further highlights the complicated nature of the pathogen × strain × cultivar × environment interaction, which remains a challenge in breeding for genetic resistance. The specific infection of each fungus and the host responses in these field trials improves our understanding of disease development and its importance in cropping systems.
An Object-Based Weighting Approach to Spatiotemporal Fusion of High Spatial Resolution Satellite Images for Small-Scale Cropland Monitoring
Authors: Park Soyeon , Park No-Wook , Na Sang-il
Published: October 2022

Continuous crop monitoring often requires a time-series set of satellite images. Since satellite images have a trade-off in spatial and temporal resolution, spatiotemporal image fusion (STIF) has been applied to construct time-series images at a consistent scale. With the increased availability of high spatial resolution images, it is necessary to develop a new STIF model that can effectively reflect the properties of high spatial resolution satellite images for small-scale crop field monitoring. This paper proposes an advanced STIF model using a single image pair, called high spatial resolution image fusion using object-based weighting (HIFOW), for blending high spatial resolution satellite images. The four-step weighted-function approach of HIFOW includes (1) temporal relationship modeling, (2) object extraction using image segmentation, (3) weighting based on object information, and (4) residual correction to quantify temporal variability between the base and prediction dates and also represent both spectral patterns at the prediction date and spatial details of fine-scale images. The specific procedures tailored for blending fine-scale images are the extraction of object-based change and structural information and their application to weight determination. The potential of HIFOW was evaluated from the experiments on agricultural sites using Sentinel-2 and RapidEye images. HIFOW was compared with three existing STIF models, including the spatial and temporal adaptive reflectance fusion model (STARFM), flexible spatiotemporal data fusion (FSDAF), and Fit-FC. Experimental results revealed that the HIFOW prediction could restore detailed spatial patterns within crop fields and clear crop boundaries with less spectral distortion, which was not represented in the prediction results of the other three models. Consequently, HIFOW achieved the best prediction performance in terms of accuracy and structural similarity for all the spectral bands. Other than the reflectance prediction, HIFOW also yielded superior prediction performance for blending normalized difference vegetation index images. These findings indicate that HIFOW could be a potential solution for constructing high spatial resolution time-series images in small-scale croplands.
Comparative Study of Pain-Related Responses of Male Piglets up to Seven Days of Age to the Application of Different Local Anaesthetics and Subsequent Castration
Authors: Söbbeler Franz Josef , Wendt Sören , Briese Andreas , Tünsmeier Julia , Waldmann Karl-Heinz , Kästner Sabine Beate Rita , von Altrock Alexandra
Published: October 2022

To evaluate pain responses to intratesticular and subscrotal injection of three local anaesthetics and their efficacy during castration a randomized controlled study was conducted. In groups of 20 piglets, procaine (2%), lidocaine (2%), or mepivacaine (2%) were administered subscrotal and intratesticularly in two different dosages: 0.5 mL of the original substances or the maximum recommended dosage according to body weight diluted with isotonic saline to a volume of 0.3 mL per each injection site. Two placebo groups received the equivalent volume of isotonic saline. A control group was injected intramuscularly with 0.5 mL isotonic saline for injection pain comparison. Electroencephalographic changes, respiratory rate, heart rate and its variability, blood pressure, and nocifensive movements were assessed in superficial isoflurane anaesthesia. While EEG-changes and linear measures of heart rate variability did not appear conclusive, the low frequency/high frequency (LF/HF) ratio corresponded best with the other pain indicators recorded. The injection of 0.3 mL diluted local anaesthetic per injection site elicited significant fewer signs of pain compared to intramuscular injection of saline. However, pain reduction, but not complete pain elimination, during castration could only be achieved with 0.5 mL of the 2% local anaesthetics per injection site, whereby lidocaine and mepivacaine were the most effective.
The Complexity of the Human–Animal Bond: Empathy, Attachment and Anthropomorphism in Human–Animal Relationships and Animal Hoarding
Authors: Prato-Previde Emanuela , Basso Ricci Elisa , Colombo Elisa Silvia
Published: October 2022

The human–animal relationship is ancient, complex and multifaceted. It may have either positive effects on humans and animals or poor or even negative and detrimental effects on animals or both humans and animals. A large body of literature has investigated the beneficial effects of this relationship in which both human and animals appear to gain physical and psychological benefits from living together in a reciprocated interaction. However, analyzing the literature with a different perspective it clearly emerges that not rarely are human–animal relationships characterized by different forms and levels of discomfort and suffering for animals and, in some cases, also for people. The negative physical and psychological consequences on animals’ well-being may be very nuanced and concealed, but there are situations in which the negative consequences are clear and striking, as in the case of animal violence, abuse or neglect. Empathy, attachment and anthropomorphism are human psychological mechanisms that are considered relevant for positive and healthy relationships with animals, but when dysfunctional or pathological determine physical or psychological suffering, or both, in animals as occurs in animal hoarding. The current work reviews some of the literature on the multifaceted nature of the human–animal relationship; describes the key role of empathy, attachment and anthropomorphism in human–animal relationships; seeks to depict how these psychological processes are distorted and dysfunctional in animal hoarding, with highly detrimental effects on both animal and human well-being.
Enriching Zoo-Housed Ring-Tailed Lemurs (Lemur catta): Assessing the Influence of Three Types of Environmental Enrichment on Behavior
Authors: Caselli Marta , Messeri Patrizia , Dessì-Fulgheri Francesco , Bandoli Francesca
Published: October 2022

Environmental enrichment is a management tool used to promote positive animal welfare by stimulating species-specific behaviors and providing animals with opportunities to exert choice and control over the environment. Our study aimed to evaluate the combined effect of three enrichment types and environmental/individual factors (i.e., individual age and rank position) on the behavior of six adult Lemur catta hosted at Pistoia Zoo (Italy). We collected data from June to September 2013 using a within-subject experimental design consisting of five conditions: Baseline, Food-based enrichment, Physical enrichment, Auditory enrichment and No enrichment provided. We conducted six 30-minute observation sessions per sampling day (total = 107 h). We recorded the animals’ behavior via 2-minute focal animal sampling per individual per observation period and analyzed data with Generalized Linear Models. The study group only performed normal species-specific behaviors. Enrichments decreased stress-related behavioral patterns, whreas environmental and individual factors influenced the other recorded behaviors. Our study confirmed the usefulness of employing an integrated methodological approach to enrichment assessment for enhancing captive lemur care.
A Standard Procedure for In Vitro Digestion Using Rumen Fermenters: A Collaborative Study
Authors: Camacho Larissa Frota , da Silva Tadeu Eder , Rodrigues João Paulo Pacheco , Franco Marcia de Oliveira , Detmann Edenio
Published: October 2022

Our objective was to propose and evaluate a standard procedure for the evaluation of in vitro dry matter digestibility for ruminant feeds, using artificial fermenters. A collaborative study was performed with seven feed analysis laboratories and four feeds (Tifton 85 hay, corn silage, soybean hulls, and soybean meal). Two types of artificial fermenters were evaluated (DaisyII Ankom and TE-150 Tecnal). Each laboratory received 80 sealed filter bags with samples (20 per feed), eight blank filter bags, a plastic bag with buffer solution reagents, and instructions describing how to conduct a 48 h in vitro assay using an artificial fermenter and how to collect bovine ruminal inoculum. On average, the contribution of laboratory effect to the total random variance was 24%, being less than the contribution of equipment (42%) and error (34%). The repeatability ranged from 3.34 to 5.79%, across feeds. The reproducibility ranged from 5.93 to 8.94% across feeds, which implied Horwitz ratios ranging from 2.94 to 4.10. Due to the specific characteristics of the analytical entity evaluated here, which is defined by the method itself, the proposed method was considered reproducible. The results highlighted that, if the method is followed exactly, its results are precise and present adequate levels of repeatability and reproducibility.
The Effect of Chiropractic Treatment on Limb Lameness and Concurrent Axial Skeleton Pain and Dysfunction in Horses
Authors: Maldonado Mikaela D. , Parkinson Samantha D. , Story Melinda R. , Haussler Kevin K.
Published: October 2022

Chiropractic care is a common treatment modality used in equine practice to manage back pain and stiffness but has limited evidence for treating lameness. The objective of this blinded, controlled clinical trial was to evaluate the effect of chiropractic treatment on chronic lameness and concurrent axial skeleton pain and dysfunction. Two groups of horses with multiple limb lameness (polo) or isolated hind limb lameness (Quarter Horses) were enrolled. Outcome measures included subjective and objective measures of lameness, spinal pain and stiffness, epaxial muscle hypertonicity, and mechanical nociceptive thresholds collected on days 0, 14, and 28. Chiropractic treatment was applied on days 0, 7, 14, and 21. No treatment was applied to control horses. Data was analyzed by a mixed model fit separately for each response variable (p < 0.05) and was examined within each group of horses individually. Significant treatment effects were noted in subjective measures of hind limb and whole-body lameness scores and vertebral stiffness. Limited or inconsistent therapeutic effects were noted in objective lameness scores and other measures of axial skeleton pain and dysfunction. The lack of pathoanatomical diagnoses, multilimb lameness, and lack of validated outcome measures likely had negative impacts on the results.
Epidemiological Analysis and Genetic Characterization of Parvovirus in Ducks in Northern Vietnam Reveal Evidence of Recombination
Authors: Dong Hieu Van , Tran Giang Thi Huong , Nguyen Huong Thi Thu , Nguyen Tuong Manh , Trinh Dai Quang , Le Van Phan , Choowongkomon Kiattawee , Rattanasrisomporn Jatuporn
Published: October 2022

In total, 130 tissue-pooled samples collected from ducks in some provinces/cities in north Vietnam were examined for waterfowl parvovirus genome identification. Twenty-six (20%) samples were positive for the parvovirus infection, based on polymerase chain reaction analysis. Of the 38 farms tested, 14 (36.84%) were positive for the waterfowl parvovirus genome. The rate of the parvovirus genome detection in ducks aged 2–4 weeks (37.04%) was significantly (p < 0.05) higher than that at ages <2 weeks (9.09%) and >4 weeks (16.30%). The positive rate on medium-scale farms (9.36%) was significantly (p < 0.05) lower than for small-scale (31.03%) and large-scale (29.73%) farms. The lengths of the four Vietnamese waterfowl parvovirus genomes identified were 4750 nucleotides. Among the four Vietnamese parvovirus genomes, nucleotide identities were from 99.29% to 99.87%. Phylogenetic analysis of the near-complete genomes indicated that the waterfowl circulating in northern Vietnam belonged to the novel goose parvovirus (NGPV) group. The Vietnamese NGPV group was closely related to the Chinese group. Recombination analysis suggested that the Vietnam/VNUA-26/2021 strain was generated by a recombination event. One positive selection site of the capsid protein was detected.
Effects of Crotonylation on Reprogramming of Cashmere Goat Somatic Cells with Different Differentiation Degrees
Authors: Li Wennan , Yan Wei , Hao Fei , Hao Lingyun , Liu Dongjun
Published: October 2022

Failure in the epigenetic reprogramming of somatic cells is considered the main reason for lower cloned embryo development efficiency. Lysine crotonylation (Kcr) occupies an important position in epigenetic modification, while its effects on somatic cell reprogramming have not been reported. In this study, we detected the influence of sodium crotonate (NaCr) on the Kcr levels in three types of somatic cells (muscle-derived satellite cells, MDSCs; fetal fibroblast cells, FFCs; and ear tip fibroblast cells, EFCs). The three types of somatic cells were treated with NaCr for cloned embryo construction, and the cleavage rates and Kcr, H3K9cr, and H3K18cr levels in the cloned embryos were analyzed. The results showed that the abnormal levels of Kcr, H3K9cr, and H3K18cr were corrected in the treatment groups. Although there was no significant difference in the cloned embryo cleavage rate in the FFC treatment group, the cleavage rates of the cloned embryos in the MDSCs and EFCs treatment groups were increased. These findings demonstrated that the Kcr level was increased with NaCr treatment in somatic cells from Cashmere goat, which contributed to proper reprogramming. The reprogramming of somatic cells can be promoted and cloned embryo development can be improved through the treatment of somatic cells with NaCr.
High-Throughput Transcriptomic Profiling Reveals the Inhibitory Effect of Hydroquinine on Virulence Factors in Pseudomonas aeruginosa
Authors: Rattanachak Nontaporn , Weawsiangsang Sattaporn , Daowtak Krai , Thongsri Yordhathai , Ross Sukunya , Ross Gareth , Nilsri Nungruthai , Baldock Robert A. , Pongcharoen Sutatip , Jongjitvimol Touchkanin , Jongjitwimol Jirapas
Published: October 2022

Hydroquinine is an organic alkaloid compound that exhibits antimicrobial activity against several bacterial strains including strains of both drug-sensitive and multidrug-resistant P. aeruginosa. Despite this, the effects of hydroquinine on virulence factors in P. aeruginosa have not yet been characterized. We therefore aimed to uncover the mechanism of P. aeruginosa hydroquinine-sensitivity using high-throughput transcriptomic analysis. We further confirmed whether hydroquinine inhibits specific virulence factors using RT-qPCR and phenotypic analysis. At half the minimum inhibitory concentration (MIC) of hydroquinine (1.250 mg/mL), 254 genes were differentially expressed (97 downregulated and 157 upregulated). We found that flagellar-related genes were downregulated by between −2.93 and −2.18 Log2-fold change. These genes were consistent with the analysis of gene ontology and KEGG pathway. Further validation by RT-qPCR showed that hydroquinine significantly suppressed expression of the flagellar-related genes. By analyzing cellular phenotypes, P. aeruginosa treated with ½MIC of hydroquinine exhibited inhibition of motility (30–54% reduction) and pyocyanin production (~25–27% reduction) and impaired biofilm formation (~57–87% reduction). These findings suggest that hydroquinine possesses anti-virulence factors, through diminishing flagellar, pyocyanin and biofilm formation.
Analysis of Mouse Blood Serum in the Dynamics of U87 Glioblastoma by Terahertz Spectroscopy and Machine Learning
Authors: Vrazhnov Denis , Knyazkova Anastasia , Konnikova Maria , Shevelev Oleg , Razumov Ivan , Zavjalov Evgeny , Kistenev Yury , Shkurinov Alexander , Cherkasova Olga
Published: October 2022

In this research, an experimental U87 glioblastoma small animal model was studied. The association between glioblastoma stages and the spectral patterns of mouse blood serum measured in the terahertz range was analyzed by terahertz time-domain spectroscopy (THz-TDS) and machine learning. The THz spectra preprocessing included (i) smoothing using the Savitsky–Golay filter, (ii) outlier removing using isolation forest (IF), and (iii) Z-score normalization. The sequential informative feature-selection approach was developed using a combination of principal component analysis (PCA) and a support vector machine (SVM) model. The predictive data model was created using SVM with a linear kernel. This model was tested using k-fold cross-validation. Achieved prediction accuracy, sensitivity, specificity were over 90%. Also, a relation was established between tumor size and the THz spectral profile of blood serum samples. Thereby, the possibility of detecting glioma stages using blood serum spectral patterns in the terahertz range was demonstrated.
The DREAM Endstation at the Linac Coherent Light Source
Authors: Walter Peter , Holmes Micheal , Obaid Razib , Amores Lope , Cheng Xianchao , Cryan James P. , Glownia James M. , Li Xiang , Lin Ming-Fu , Ng May Ling , Robinson Joseph , Shivaram Niranjan , Yin Jing , Fritz David , James Justin , Castagna Jean-Charles , Osipov Timur
Published: October 2022

Free-electron lasers (FEL), with their ultrashort pulses, ultrahigh intensities, and high repetition rates at short wavelength, have provided new approaches to Atomic and Molecular Optical Science. One such approach is following the birth of a photo electron to observe ion dynamics on an ultrafast timescale. Such an approach presents the opportunity to decipher the photon-initiated structural dynamics of an isolated atomic and molecular species. It is a fundamental step towards understanding single- and non-linear multi-photon processes and coherent electron dynamics in atoms and molecules, ultimately leading to coherent control following FEL research breakthroughs in pulse shaping and polarization control. A key aspect for exploring photoinduced quantum phenomena is visualizing the collective motion of electrons and nuclei in a single reaction process, as dynamics in atoms/ions proceed at femtosecond (10−15 s) timescales while electronic dynamics take place in the attosecond timescale (10−18 s). Here, we report on the design of a Dynamic Reaction Microscope (DREAM) endstation located at the second interaction point of the Time-Resolved Molecular and Optical (TMO) instrument at the Linac Coherent Light Source (LCLS) capable of following the photon–matter interactions by detecting ions and electrons in coincidence. The DREAM endstation takes advantage of the pulse properties and high repetition rate of LCLS-II to perform gas-phase soft X-ray experiments in a wide spectrum of scientific domains. With its design ability to detect multi-ions and electrons in coincidence while operating in step with the high repetition rate of LCLS-II, the DREAM endstation takes advantage of the inherent momentum conservation of reaction product ions with participating electrons to reconstruct the original X-ray photon–matter interactions. In this report, we outline in detail the design of the DREAM endstation and its functionality, with scientific opportunities enabled by this state-of-the-art instrument.
Model for Estimating the Modulus of Elasticity of Asphalt Layers Using Machine Learning
Authors: Svilar Mila , Peško Igor , Šešlija Miloš
Published: October 2022

The management of roads, as well as their maintenance, calls for an adequate assessment of the load-bearing capacity of the pavement structure. This serves as the basis on which future maintenance requirements are planned and plays a significant role in determining whether the rehabilitation or reconstruction of the pavement structure is required. The stability of the pavement structure depends on a large number of parameters, and it is not possible to fully assess all of them when making an estimation. One of the most significant parameters is the modulus of elasticity of asphalt layers (EAC). The goal of this study is to use models based on machine learning to perform a quick and efficient assessment of the modulus of elasticity of asphalt layers, as well as to compare the formed models. The paper defines models for EAC estimation using machine learning, in which the input data include the measured deflections and the temperature of the upper surface of the asphalt layer. Analyses of modeling using artificial neural networks (ANNs), support vector machines (SVMs) and boosted regression trees (BRT) were compared. The SVM method showed a higher accuracy in estimating the EAC modulus, with a mean absolute percentage error (MAPE) of 7.64%, while the ANN method and the BRT achieved accuracies of 9.13% and 8.84%, respectively. Models formed in this way can be practically implemented in the management and maintenance of roads. They enable an adequate assessment of the remaining load-bearing capacity and the level of reliability of the pavement structure using non-destructive methods, at the same time reducing the financial costs.