bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of Anodal Transcranial Direct Current Stimulation Combined With Cognitive Training for Improving Cognition and Language Among Children With Cerebral Palsy With Cognitive Impairment: A Pilot, Randomized, Controlled, Double-Blind, and Clinical Trial
Authors: Ko Eun Jae , Hong Mi Jin , Choi Eun Jung , Yuk Jin Sook , Yum Mi Sun , Sung In Young
Published: August 2021

About 30–45% of cerebral palsy (CP) patients have cognitive impairment. Previous studies showed the evidence that transcranial direct current stimulation (tDCS) may have some benefits in attention-deficit/hyperactivity disorder, autism spectrum disorder, and motor development in CP. The aim of this study is to evaluate the effect of tDCS on cognition, language, and activities of daily living (ADL) among children with CP with cognitive impairment. It was a pilot, randomized, controlled, double-blind, clinical trial in a tertiary pediatric hospital, and 13 children with CP and a cognitive age under 42 months were enrolled. tDCS group (n = 8) had active tDCS and cognitive training (20 min/session, total 20 sessions, for 12 weeks) and sham group (n = 5) had sham tDCS and cognitive training. Primary outcome was the Bayley Scales of Infant Development II (BSID II). Secondary outcomes were the Pediatric Evaluation of Disability Inventory (PEDI), the Laboratory Temperament Assessment Battery (Lab-TAB), the Early Childhood Behavior Questionnaire (ECBQ), the Korean version of MacArthur–Bates Communicative Development Inventories (M-B CDI-K), the Sequenced Language Scale for Infants (SELSI) and the Preschool Receptive-Expressive Language Scale (PRES). After intervention, the tDCS group showed significant improvements in all measurements (p < 0.05) except the M-B CDI-K (grammar), whereas the sham group only showed significant improvements in the Lab-TAB (manipulation domain), the ECBQ (attentional shifting), and the M-B CDI-K (comprehension). The between-group differences in the degree of post-intervention improvement were not statistically significant. The degree of improvement was associated with better baseline cognitive function and younger age (p < 0.05). There were no major adverse events after tDCS. The combined application of tDCS and cognitive training was feasible and associated with improvements in cognitive function, ADL, and language among children with CP with cognitive impairment. However, considering that it is a pilot study, further larger-scale systematic investigation is needed.

Clinical Trial Registration: The trial was registered in the Clinical Research Information Service database, identifier: KCT0003023.
Applying Next-Generation Sequencing Platforms for Pharmacogenomic Testing in Clinical Practice
Authors: Tafazoli Alireza , Guchelaar Henk-Jan , Miltyk Wojciech , Kretowski Adam J. , Swen Jesse J.
Published: August 2021

Pharmacogenomics (PGx) studies the use of genetic data to optimize drug therapy. Numerous clinical centers have commenced implementing pharmacogenetic tests in clinical routines. Next-generation sequencing (NGS) technologies are emerging as a more comprehensive and time- and cost-effective approach in PGx. This review presents the main considerations for applying NGS in guiding drug treatment in clinical practice. It discusses both the advantages and the challenges of implementing NGS-based tests in PGx. Moreover, the limitations of each NGS platform are revealed, and the solutions for setting up and management of these technologies in clinical practice are addressed.
Adverse Cardiovascular Effects of Anti-COVID-19 Drugs
Authors: Liu Dongling , Zeng Xiang , Ding Zufeng , Lv Fenghua , Mehta Jawahar L. , Wang Xianwei
Published: August 2021

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) or COVID-19 infection is the cause of the ongoing global pandemic. Mortality from COVID-19 infection is particularly high in patients with cardiovascular diseases. In addition, COVID-19 patients with preexisting cardiovascular comorbidities have a higher risk of death. Main cardiovascular complications of COVID-19 are myocardial infarction, myocarditis, acute myocardial injury, arrhythmias, heart failure, stroke, and venous thromboembolism. Therapeutic interventions in terms of drugs for COVID-19 have many cardiac adverse effects. Here, we review the relative therapeutic efficacy and adverse effects of anti-COVID-19 drugs.
The Marine Gastropod Crepidula fornicata Remains Resilient to Ocean Acidification Across Two Life History Stages
Authors: Reyes-Giler Christopher L. , Benson Brooke E. , Levy Morgan , Chen Xuqing , Pires Anthony , Pechenik Jan A. , Davies Sarah W.
Published: August 2021

Rising atmospheric CO2reduces seawater pH causing ocean acidification (OA). Understanding how resilient marine organisms respond to OA may help predict how community dynamics will shift as CO2continues rising. The common slipper shell snailCrepidula fornicatais a marine gastropod native to eastern North America that has been a successful invader along the western European coastline and elsewhere. It has also been previously shown to be resilient to global change stressors. To examine the mechanisms underlyingC. fornicata’sresilience to OA, we conducted two controlled laboratory experiments. First, we examined several phenotypes and genome-wide gene expression ofC. fornicatain response to pH treatments (7.5, 7.6, and 8.0) throughout the larval stage and then tested how conditions experienced as larvae influenced juvenile stages (i.e., carry-over effects). Second, we examined genome-wide gene expression patterns ofC. fornicatalarvae in response to acute (4, 10, 24, and 48 h) pH treatment (7.5 and 8.0). BothC. fornicatalarvae and juveniles exhibited resilience to OA and their gene expression responses highlight the role of transcriptome plasticity in this resilience. Larvae did not exhibit reduced growth under OA until they were at least 8 days old. These phenotypic effects were preceded by broad transcriptomic changes, which likely served as an acclimation mechanism for combating reduced pH conditions frequently experienced in littoral zones. Larvae reared in reduced pH conditions also took longer to become competent to metamorphose. In addition, while juvenile sizes at metamorphosis reflected larval rearing pH conditions, no carry-over effects on juvenile growth rates were observed. Transcriptomic analyses suggest increased metabolism under OA, which may indicate compensation in reduced pH environments. Transcriptomic analyses through time suggest that these energetic burdens experienced under OA eventually dissipate, allowingC. fornicatato reduce metabolic demands and acclimate to reduced pH. Carry-over effects from larval OA conditions were observed in juveniles; however, these effects were larger for more severe OA conditions and larvae reared in those conditions also demonstrated less transcriptome elasticity. This study highlights the importance of assessing the effects of OA across life history stages and demonstrates how transcriptomic plasticity may allow highly resilient organisms, likeC. fornicata, to acclimate to reduced pH environments.
Genome-Wide Association Mapping of Mixed Linkage (1,3;1,4)-β-Glucan and Starch Contents in Rice Whole Grain
Authors: Panahabadi Rahele , Ahmadikhah Asadollah , McKee Lauren S. , Ingvarsson Pär K. , Farrokhi Naser
Published: August 2021

The glucan content of rice is a key factor defining its nutritional and economic value. Starch and its derivatives have many industrial applications such as in fuel and material production. Non-starch glucans such as (1,3;1,4)-β-D-glucan (mixed-linkage β-glucan, MLG) have many benefits in human health, including lowering cholesterol, boosting the immune system, and modulating the gut microbiome. In this study, the genetic variability of MLG and starch contents were analyzed in rice (Oryza sativa L.) whole grain, by performing a new quantitative analysis of the polysaccharide content of rice grains. The 197 rice accessions investigated had an average MLG content of 252 μg/mg, which was negatively correlated with the grain starch content. A new genome-wide association study revealed seven significant quantitative trait loci (QTLs) associated with the MLG content and two QTLs associated with the starch content in rice whole grain. Novel genes associated with the MLG content were a hexose transporter and anthocyanidin 5,3-O-glucosyltransferase. Also, the novel gene associated with the starch content was a nodulin-like domain. The data pave the way for a better understanding of the genes involved in determining both MLG and starch contents in rice grains and should facilitate future plant breeding programs.
Arbuscular Mycorrhizal Symbiosis Triggers Major Changes in Primary Metabolism Together With Modification of Defense Responses and Signaling in Both Roots and Leaves of Vitis vinifera
Authors: Goddard Mary-Lorène , Belval Lorène , Martin Isabelle R. , Roth Lucie , Laloue Hélène , Deglène-Benbrahim Laurence , Valat Laure , Bertsch Christophe , Chong Julie
Published: August 2021

Grapevine (Vitis vinifera L.) is one of the most important crops worldwide but is subjected to multiple biotic and abiotic stresses, especially related to climate change. In this context, the grapevine culture could take advantage of symbiosis through association with arbuscular mycorrhizal fungi (AMF), which are able to establish symbiosis with most terrestrial plants. Indeed, it is well established that mycorrhization improves grapevine nutrition and resistance to stresses, especially water stress and resistance to root pathogens. Thus, it appears essential to understand the effect of mycorrhization on grapevine metabolism and defense responses. In this study, we combined a non-targeted metabolomic approach and a targeted transcriptomic study to analyze changes induced in both the roots and leaves of V. vinifera cv. Gewurztraminer by colonization with Rhizophagus irregularis (Ri). We showed that colonization of grapevine with AMF triggers major reprogramming of primary metabolism in the roots, especially sugar and fatty acid metabolism. On the other hand, mycorrhizal roots had decreased contents of most sugars and sugar acids. A significant increase in several fatty acids (C16:1, linoleic and linolenic acids and the C20 arachidonic and eicosapentaenoic acids) was also detected. However, a downregulation of the JA biosynthesis pathway was evidenced. We also found strong induction of the expression of PR proteins from the proteinase inhibitor (PR6) and subtilase (PR7) families in roots, suggesting that these proteins are involved in the mycorrhiza development but could also confer higher resistance to root pathogens. Metabolic changes induced by mycorrhization were less marked in leaves but involved higher levels of linoleic and linolenic acids and decreased sucrose, quinic, and shikimic acid contents. In addition, Ri colonization resulted in enhanced JA and SA levels in leaves. Overall, this study provides a detailed picture of metabolic changes induced by AMF colonization in a woody, economically important species. Moreover, stimulation of fatty acid biosynthesis and PR protein expression in roots and enhanced defense hormone contents in leaves establish first insight in favor of better resistance of grapevine to various pathogens provided by AMF colonization.
Fear of Evaluation and Online Self-Disclosure on WeChat: Moderating Effects of Protective Face Orientation
Authors: Zeng Runxi , Zhu Di
Published: August 2021

Fear of evaluation is a key factor that affects how social media users present themselves to others, but little is known about the effects and mechanisms involved, especially on the relationship between fear of positive evaluation and online self-disclosure. This study explores how fear of evaluation affects online self-disclosure and examines how this relationship is moderated by protective face orientation in the Chinese context. A total of 750 Chinese WeChat users constituted the sample for a questionnaire-based analysis and regression analysis. The results showed that both fear of positive evaluation and fear of negative evaluation had a significant negative effect on the amount of online self-disclosure and a significant positive effect on the depth of online self-disclosure. Protective face orientation had a moderating effect on the relationship between fear of evaluation and online self-disclosure for both the amount and depth of online self-disclosure. Our findings suggest that social network site (SNS) users' fear of evaluation can be attributed to their cognitive attitude toward the external environment, and the loss of face in the Chinese context can be included in the social context.
Susceptibility to Ingroup Influence in Adolescents With Intellectual Disability: A Minimal Group Experiment on Social Judgment Making
Authors: Egger Sara
Published: August 2021

Adolescents with intellectual disability (ID) experience challenges and uncertainty when making judgments about other people’s intentions. In an attempt to achieve certainty, they might exhibit judgment tendencies that differ from those of typically developing adolescents. This study investigated social judgment making in adolescents with ID (n = 34, Mage = 14.89 years, SD = 1.41 years) compared with chronological age-matched adolescents without ID (n = 34, Mage = 14.68 years, SD = 1.15 years) and mental age (MA)-matched children (n = 34, Mage = 7.93 years, SD = 0.64 years). Participants used a computer-based task to judge the hostility of persons (fictitious characters). Adolescents with ID were found to make more polarizing judgments (i.e., either positive or negative, as opposed to moderate judgments) and were more likely to be guided by the opinions of a fictitious peer ingroup (minimal group) compared with adolescents without ID. No such differences were found between adolescents with ID and MA-matched children. The results are discussed in terms of scientific and practical implications.
Restrictive Deterrence in Drug Offenses: A Systematic Review and Meta-Synthesis of Mixed Studies
Authors: Guan Xin , Lo T. Wing
Published: August 2021

Deterrence by punishment aims to prevent a crime; however, it is not always successful. Restrictive deterrence explains the continuous criminal activities that occur despite deterrence; offenders enact various strategies to avoid detection, which is more typical among drug offenders given that they have a high frequency of offending and exposure to punishment. This systematic review provides an in-depth understanding of restrictive deterrence of drug offenders. Two prominent themes, “restrictive deterrence strategy” and “deterrability and restrictive deterrence,” depict drug offenders' restrictive deterrence and effectively fit within the certainty–severity framework of punishment. Future studies should investigate restrictive deterrence strategies in the after-arrest context, the facilitative effect of perception of risk on strategy development, and facilitators or inhibitors affecting the diffusion of restrictive deterrence strategies.
Interoception and Obsessive-Compulsive Disorder: A Review of Current Evidence and Future Directions
Authors: Bragdon Laura B. , Eng Goi Khia , Belanger Amanda , Collins Katherine A. , Stern Emily R.
Published: August 2021

Disrupted interoceptive processes are present in a range of psychiatric conditions, and there is a small but growing body of research on the role of interoception in obsessive-compulsive disorder (OCD). In this review, we outline dimensions of interoception and review current literature on the processing of internal bodily sensations within OCD. Investigations in OCD utilizing objective measures of interoception are limited and results mixed, however, the subjective experience of internal bodily sensations appears to be atypical and relate to specific patterns of symptom dimensions. Further, neuroimaging investigations suggest that interoception is related to core features of OCD, particularly sensory phenomena and disgust. Interoception is discussed in the context of treatment by presenting an overview of existing interventions and suggesting how modifications aimed at better targeting interoceptive processes could serve to optimize outcomes. Interoception represents a promising direction for multi-method research in OCD, which we expect, will prove useful for improving current interventions and identifying new treatment targets.
Mental Disorders, Cognitive Impairment and the Risk of Suicide in Older Adults
Authors: Kułak-Bejda Agnieszka , Bejda Grzegorz , Waszkiewicz Napoleon
Published: August 2021

More than 600 million people are aged 60 years and over are living in the world. The World Health Organization estimates that this number will double by 2025 to 2 billion older people. Suicide among people over the age of 60 is one of the most acute problems. The factors strongly associated with suicide are mentioned: physical illnesses, such as cancer, neurologic disorder, pain, liver disease, genital disorders, or rheumatoid disorders. Moreover, neurologic conditions, especially stroke, may affect decision-making processes, cognitive capacity, and language deficit. In addition to dementia, the most common mental disorders are mood and anxiety disorders. A common symptom of these disorders in the elderly is cognitive impairment. This study aimed to present the relationship between cognitive impairment due to dementia, mood disorders and anxiety, and an increased risk of suicide among older people. Dementia is a disease where the risk of suicide is significant. Many studies demonstrated that older adults with dementia had an increased risk of suicide death than those without dementia. Similar conclusions apply to prodromal dementia Depression is also a disease with a high risk of suicide. Many researchers found that a higher level of depression was associated with suicide attempts and suicide ideation. Bipolar disorder is the second entity in mood disorders with an increased risk of suicide among the elderly. Apart from suicidal thoughts, bipolar disorder is characterized by high mortality. In the group of anxiety disorders, the most significant risk of suicide occurs when depression is present. In turn, suicide thoughts are more common in social phobia than in other anxiety disorders. Suicide among the elderly is a serious public health problem. There is a positive correlation between mental disorders such as dementia, depression, bipolar disorder, or anxiety and the prevalence of suicide in the elderly. Therefore, the elderly should be comprehensively provided with psychiatric and psychological support.
The Prevalence of Psychological Symptoms in Pregnant Healthcare Workers (HCWs) and Pregnant Non-HCWs During the Early Stage of COVID-19 Pandemic in Chongqing, China
Authors: Liu Min , Li Nan , Cai Xianghao , Feng Xiaoyan , Wang Rong , Xiong Peng
Published: August 2021

Background: Studies showed that healthcare workers (HCWs) and pregnant women bore the burden of mental problems during the coronavirus disease 2019 (COVID-19) pandemic. While, few studies have focused on the psychological impact of COVID-19 pandemic on pregnant women who work at healthcare settings. This study aimed to investigate and compare the prevalence difference of psychological symptoms between pregnant HCWs and pregnant non-HCWs during the early stage of COVID-19 pandemic in China.

Methods: A cross-sectional online survey with anonymous structured questionnaires was conducted from February 15 to March 9, 2020. A total of 205 pregnant women in Chongqing, China were recruited. The mental health status was assessed using symptom checklist-90 (SCL-90).

Results: Our sample was composed of 83 pregnant HCWs (mean age = 29.8) and 122 pregnant non-HCWs (mean age = 30.8). The results suggested the prevalence of psychological symptoms (the factor score ≥2) among all pregnant women ranged from 6.83% (psychosis symptoms) to 17.56% (obsessive-compulsive symptoms). Compared with pregnant non-HCWs, pregnant HCWs reported higher prevalence of psychological symptoms in 10 factors of SCL-90. After controlling the confounding variables, multiple logistic regression demonstrated that pregnant HCWs experienced higher prevalence of psychological symptoms of somatization (18.07 vs. 5.74%, p = 0.006, aOR = 4.52), anxiety disorders (16.87 vs. 6.56%, p = 0.016, aOR = 3.54), and hostility (24.10 vs. 10.66%, p = 0.027, aOR = 2.70) than those among pregnant non-HCW.

Conclusion: Our study indicated that pregnant HCWs were more likely to suffer from mental health distress than pregnant non-HCWs during the early stage of COVID-19 pandemic. It is vital to implement targeted psychological interventions for pregnant women, especially for pregnant HCWs to cope with distress when facing the emerging infectious diseases.
Comorbid Premenstrual Dysphoric Disorder and Bipolar Disorder: A Review
Authors: Slyepchenko Anastasiya , Minuzzi Luciano , Frey Benicio N.
Published: August 2021

Bipolar disorder (BD) differs in its clinical presentation in females compared to males. A number of clinical characteristics have been associated with BD in females: more rapid cycling and mixed features; higher number of depressive episodes; and a higher prevalence of BD type II. There is a strong link between BD and risk for postpartum mood episodes, and a substantial percentage of females with BD experience premenstrual mood worsening of varying degrees of severity. Females with premenstrual dysphoric disorder (PMDD)—the most severe form of premenstrual disturbances—comorbid with BD appear to have a more complex course of illness, including increased psychiatric comorbidities, earlier onset of BD, and greater number of mood episodes. Importantly, there may be a link between puberty and the onset of BD in females with comorbid PMDD and BD, marked by a shortened gap between the onset of BD and menarche. In terms of neurobiology, comorbid BD and PMDD may have unique structural and functional neural correlates. Treatment of BD comorbid with PMDD poses challenges, as the first line treatment of PMDD in the general population is selective serotonin reuptake inhibitors, which produce risk of treatment-emergent manic symptoms. Here, we review current literature concerning the clinical presentation, illness burden, and unique neurobiology of BD comorbid with PMDD. We additionally discuss obstacles faced in symptom tracking, and management of these comorbid disorders.
TNF-Alpha as an Initiator of Allodynia and Anxiety-Like Behaviors in a Preclinical Model of PTSD and Comorbid Pain
Authors: Dib Patrick , Zhang Yong , Ihnat Michael A. , Gallucci Randle M. , Standifer Kelly M.
Published: August 2021

Post-Traumatic Stress Disorder (PTSD) is a debilitating mental health disorder that occurs after exposure to a traumatic event. Patients with comorbid chronic pain experience affective distress, worse quality of life, and poorer responses to treatments for pain or PTSD than those with either condition alone. FDA-approved PTSD treatments are often ineffective analgesics, requiring additional drugs to treat co-morbid symptoms. Therefore, development of new treatment strategies necessitate a better understanding of the pathophysiology of PTSD and comorbid pain. The single prolonged stress (SPS) model of PTSD induces the development of persistent mechanical allodynia and thermal hyperalgesia. Increased Nociceptin/Orphanin FQ (N/OFQ) levels in serum and CSF accompany these exaggerated nociceptive responses, as well as increased serum levels of the pro-inflammatory cytokine tumor necrosis factor (TNF-α). Therefore, the primary goal was to determine the role of TNF-α in the development of SPS-induced allodynia/hyperalgesia and elevated serum and CNS N/OFQ using two approaches: TNF-α synthesis inhibition, and blockade with anti-TNF-α antibody that acts primarily in the periphery. Administration of TNF-α synthesis blocker, thalidomide (THL), immediately after SPS prevented increased TNF-α and development of allodynia and hyperalgesia. The THL effect lasted at least 21 days, well after thalidomide treatment ended (day 5). THL also prevented SPS-induced increases in serum N/OFQ and reversed regional N/OFQ mRNA expression changes in the CNS. Serum TNF-α increases detected at 4 and 24 h post SPS were not accompanied by blood brain barrier disruption. A single injection of anti-TNF-α antibody to male and female rats during the SPS procedure prevented the development of allodynia, hyperalgesia, and elevated serum N/OFQ, and reduced SPS-induced anxiety-like behaviors in males. Anti-TNFα treatment also blocked development of SPS-induced allodynia in females, and blocked increased hypothalamic N/OFQ in males and females. This suggests that a peripheral TNF-α surge is necessary for the initiation of allodynia associated with SPS, as well as the altered central and peripheral N/OFQ that maintains nociceptive sensitivity. Therefore, early alleviation of TNF-α provides new therapeutic options for investigation as future PTSD and co-morbid pain treatments.
The Association Between Internet Addiction and Anxiety in Nursing Students: A Network Analysis
Authors: Cai Hong , Xi Hai-Tao , An Fengrong , Wang Zhiwen , Han Lin , Liu Shuo , Zhu Qianqian , Bai Wei , Zhao Yan-Jie , Chen Li , Ge Zong-Mei , Ji Mengmeng , Zhang Hongyan , Yang Bing-Xiang , Chen Pan , Cheung Teris , Jackson Todd , Tang Yi-Lang , Xiang Yu-Tao
Published: August 2021

Background: Nursing students who suffer from co-occurring anxiety experience added difficulties when communicating and interacting with others in a healthy, positive, and meaningful way. Previous studies have found strong positive correlations between Internet addiction (IA) and anxiety, suggesting that nursing students who report severe IA are susceptible to debilitating anxiety as well. To date, however, network analysis (NA) studies exploring the nature of association between individual symptoms of IA and anxiety have not been published.

Objective: This study examined associations between symptoms of IA and anxiety among nursing students using network analysis.

Methods: IA and anxiety symptoms were assessed using the Internet Addiction Test (IAT) and the Generalized Anxiety Disorder Screener (GAD-7), respectively. The structure of IA and anxiety symptoms was characterized using “Strength” as a centrality index in the symptom network. Network stability was tested using a case-dropping bootstrap procedure and a Network Comparison Test (NCT) was conducted to examine whether network characteristics differed on the basis of gender and by region of residence.

Results: A total of 1,070 nursing students participated in the study. Network analysis showed that IAT nodes, “Academic decline due to Internet use,” “Depressed/moody/nervous only while being off-line,” “School grades suffer due to Internet use,” and “Others complain about your time spent online” were the most influential symptoms in the IA-anxiety network model. Gender and urban/rural residence did not significantly influence the overall network structure.

Conclusion: Several influential individual symptoms including Academic declines due to Internet use, Depressed/moody/nervous only while being off-line, School grades suffering due to Internet use and Others complain about one's time spent online emerged as potential targets for clinical interventions to reduce co-occurring IA and anxiety. Additionally, the overall network structure provides a data-based hypothesis for explaining potential mechanisms that account for comorbid IA and anxiety.
The Association of Social Capital and Self-Rated Health Between Urban Residents and Urbanized Rural Residents in Southwest China
Authors: Ma Tianpei , Gao Bo
Published: August 2021

China has seen an accelerated process of urbanization in the past 30 years. The influence of urbanization on health is complex and primarily influenced by changes in social capital. The purpose of this research was to compare the social capital between urban residents and urbanized rural residents of southwest China and its relationship with self-rated health. It is of great significance to study the difference of social capital between urban and urbanized rural residents to help urbanized rural residents improve their social adaptability and health. Data was collected from 1,646 residents between November and December of 2017 in Chengdu. Three logistic regressions were used to investigate the association between social capital and self-rated health by controlling for demographic variables, lifestyles factors, and health status factors. We observed that urban residents' self-rated health had a higher proportion of “good” than that of urbanized rural residents (P = 0.017). After controlling for factors such as health status and demographic characteristics, participants with higher social capital had better self-rated health. Urbanized rural residents with higher community trust and belonging had better self-rated health (OR = 0.701, 95% CI = 0.503~0.978), however urban residents with higher personal social networks and family relationships had better self-rated health (OR = 0.676, 95% CI = 0.490~0.933 and OR = 0.666, 95% CI = 0.450~0.987, respectively). Different types of communities should focus on the types of social capital from different sources, so as to take more targeted measures to improve the social support of residents and improve their health. Improving residents' social trust and sense of belonging may help urbanized rural residents better adapt to the new living environment and help them complete the identity transformation.
An Introduction to the Main Types of Economic Evaluations Used for Informing Priority Setting and Resource Allocation in Healthcare: Key Features, Uses, and Limitations
Authors: Turner Hugo C. , Archer Rachel A. , Downey Laura E. , Isaranuwatchai Wanrudee , Chalkidou Kalipso , Jit Mark , Teerawattananon Yot
Published: August 2021

Economic evidence is increasingly being used for informing health policies. However, the underlining principles of health economic analyses are not always fully understood by non-health economists, and inappropriate types of analyses, as well as inconsistent methodologies, may be being used for informing health policy decisions. In addition, there is a lack of open access information and methodological guidance targeted to public health professionals, particularly those based in low- and middle-income country (LMIC) settings. The objective of this review is to provide a comprehensive and accessible introduction to economic evaluations for public health professionals with a focus on LMIC settings. We cover the main principles underlining the most common types of full economic evaluations used in healthcare decision making in the context of priority setting (namely cost-effectiveness/cost-utility analyses, cost-benefit analyses), and outline their key features, strengths and weaknesses. It is envisioned that this will help those conducting such analyses, as well as stakeholders that need to interpret their output, gain a greater understanding of these methods and help them select/distinguish between the different approaches. In particular, we highlight the need for greater awareness of the methods used to place a monetary value on the health benefits of interventions, and the potential for such estimates to be misinterpreted. Specifically, the economic benefits reported are typically an approximation, summarising the health benefits experienced by a population monetarily in terms of individual preferences or potential productivity gains, rather than actual realisable or fiscal monetary benefits to payers or society.
Earth Imaging From the Surface of the Moon With a DSCOVR/EPIC-Type Camera
Authors: Gorkavyi Nick , Carn Simon , DeLand Matt , Knyazikhin Yuri , Krotkov Nick , Marshak Alexander , Myneni Ranga , Vasilkov Alexander
Published: August 2021

The Earth Polychromatic Imaging Camera (EPIC) on the Deep Space Climate Observatory (DSCOVR) satellite observes the entire Sun-illuminated Earth from sunrise to sunset from the L1 Sun-Earth Lagrange point. The L1 location, however, confines the observed phase angles to ∼2°–12°, a nearly backscattering direction, precluding any information on the bidirectional surface reflectance factor (BRF) or cloud/aerosol phase function. Deploying an analog of EPIC on the Moon’s surface would offer a unique opportunity to image the full range of Earth phases, including observing ocean/cloud glint reflection for different phase angles; monitoring of transient volcanic clouds; detection of circum-polar mesospheric and stratospheric clouds; estimating the surface BRF and full phase-angle integrated albedo; and monitoring of vegetation characteristics for different phase angles.
Racism on the Brain
Authors: Weichselbaum Claire , Banks Kira Hudson
Published: August 2021

Racism continues to be a significant problem in much of the world. Understanding how racism happens in the brain may help us find ways to overcome it. Our brains often categorize people as similar to, or different from, ourselves. A part of the brain called the amygdala becomes active when we see someone who we think is different, and this can lead to treating that person unfairly. But another part of the brain called the prefrontal cortex is able to calm the amygdala, and we can boost its power using a thinking strategy called cognitive reappraisal. Even better, we may be able to prevent people from becoming racist by teaching kids to appreciate differences, so their amygdala does not get activated by people different from themselves. Combined with fixing unfair rules and systems around us, these brain strategies may help us to defeat racism.
Defining Color Change in Pitaya: A Close Look at Betacyanin Synthesis Genes in Stenocereus queretaroensis
Authors: Morales Javier , Araujo-Sanchez Jorge , Castro-Concha Lizbeth , Ku Angela , Pereira-Santana Alejandro , Miranda-Ham María de Lourdes , Castaño Enrique
Published: August 2021

Betalains are tyrosine-derived plant pigments present in several species of the Caryophyllales order. Betalains are classified in red betacyanins and yellow betaxanthins and are implicated in plant stress tolerance and visual attraction for pollinators. The compounds are used as natural colorants in many industries. Today, there is little information on betalain biosynthesis with several key enzymes that remain unknown on plants of the Caryophyllales order. Omic tools have proven to be very useful in gaining insights into various molecular mechanisms. In this study, we used suspension cells from fruits of the cactus Stenocereus queretaroensis. Two growing conditions were used to perform RNA-seq and differential expression analysis to help identify betalain biosynthesis-related genes. We found 98 differential expressed genes related to aromatic amino acids and betalain biosynthesis pathways. Interestingly, we found that only one gene of the betalain synthesis pathway was differentially expressed. The rest of the genes belong to the aromatic amino acid pathway, including hydroxy phenylpyruvate-related genes, suggesting the possibility of an alternative biosynthetic pathway similar to that observed in legumes.
10-Year Anniversary of the European Proximity Operations Simulator 2.0—Looking Back at Test Campaigns, Rendezvous Research and Facility Improvements
Authors: Rems Florian , Frei Heike , Risse Eicke-Alexander , Burri Matthias
Published: August 2021

Completed in 2009, the European Proximity Operations Simulator 2.0 (EPOS 2.0) succeeded EPOS 1.0 at the German Space Operations Center (GSOC). One of the many contributions the old EPOS 1.0 facility made to spaceflight rendezvous is the verification of the Jena-Optronik laser-based sensors used by the Automated Transfer Vehicle. While EPOS 2.0 builds upon its heritage, it is a completely new design aiming at considerably more complex rendezvous scenarios. During the last ten years, GSOC’s On-Orbit-Servicing & Autonomy group, who operates, maintains and evolves EPOS 2.0, has made numerous contributions to the field of uncooperative rendezvous, using EPOS as its primary tool. After general research in optical navigation in the early 2010s, the OOS group took a leading role in the DLR project “On-Orbit-Servicing End-to-End Simulation” in 2014. EPOS 2.0 served as the hardware in the loop simulator of the rendezvous phase and contributed substantially to the project’s remarkable success. Over the years, E2E has revealed demanding requirements, leading to numerous facility improvements and extensions. In addition to the OOS group’s research work, numerous and diverse open-loop test campaigns for industry and internal (DLR) customers have shaped the capabilities of EPOS 2.0 significantly.
Determining the Effects of Serial Injections of Pregnant Mare Serum Gonadotropin on Plasma Testosterone Concentrations, Testicular Dynamics, and Semen Production in Leopard Geckos (Eublepharis macularius)
Authors: Mason Alexandra K. , Lee Jeongha , Perry Sean M. , Boykin Kimberly L. , Del Piero Fabio , Lierz Michael , Mitchell Mark A.
Published: August 2021

Reptiles are highly susceptible to anthropogenic activities as a result of their narrow geographical ranges and habitat specialization, making them a conservation concern. Geckos represent one of the mega-diverse reptile lineages under pressure; however, limited assisted reproductive technologies currently exist for these animals. Exogenous pregnant mare serum gonadotropin (PMSG) has been found to exhibit follicle stimulating hormone-like action and has been routinely used to alter reproductive hormones of vertebrates in assisted reproductive protocols. The purpose of this study was to determine the effects of serial injections of 20 IU and 50 IU PMSG on circulating testosterone concentrations, testicular dynamics, and semen production in a model species of gecko. Twenty-four captive-bred, adult, male leopard geckos (Eublepharis macularius) were divided into three treatment groups and administered a once-weekly injection of either PMSG or saline for a total of nine weeks. Ultrasonographic testicular measurements, electrostimulation for semen collection, and venipuncture were performed on days 0, 21, 42, and 63. Right unilateral orchidectomies and epididymectomies were performed in all animals on day 63; tissues were submitted for histopathology. PMSG treated geckos had significantly higher testicular volumes and weights, spermatozoa motility, and spermatozoa concentrations compared with controls. However, there were no significant differences in testosterone concentrations by treatment or time. Under the conditions outlined, PMSG is effective at stimulating spermatogenesis and increasing testicular size, but not effective at increasing testosterone concentrations in the leopard gecko between October–December in the Northern hemisphere.
Peptidomics Analysis of Virulent Peptides Involved in Streptococcus suis Pathogenesis
Authors: Chaiden Chadaporn , Jaresitthikunchai Janthima , Phaonakrop Narumon , Roytrakul Sittiruk , Kerdsin Anusak , Nuanualsuwan Suphachai
Published: August 2021

Streptococcus suis (S. suis) is a zoonotic pathogen causing severe streptococcal disease worldwide. S. suis infections in pigs and humans are frequently associated with the virulent S. suis serotype 2 (SS2). Though various virulence factors of S. suis have been proposed, most of them were not essentially accounted for in the experimental infections. In the present study, we compared the peptidomes of highly virulent SS2 and SS14 in humans, the swine causative serotypes SS7 and SS9, and the rarely reported serotypes SS25 and SS27, and they were cultured in a specified culture medium containing whole blood to simulate their natural host environment. LC-MS/MS could identify 22 unique peptides expressed in the six S. suis serotypes. Under the host-simulated environment, peptides from the ABC-type phosphate transport system (SSU05_1106) and 30S ribosomal protein S2 (rpsB) were detected in the peptidome of virulent SS2 and SS14. Therefore, we suggest that these two proteins or their derived peptides might be involved in the survival of S. suis when simulated with a blood environment.
Modified Spirulina maxima Pectin Nanoparticles Improve the Developmental Competence of In Vitro Matured Porcine Oocytes
Authors: Roy Pantu-Kumar , Qamar Ahmad-Yar , Tanga Bereket-Molla , Bang Seonggyu , Seong Gyeonghwan , Fang Xun , Kim Ghangyong , Edirisinghe Shan-Lakmal , De Zoysa Mahanama , Kang Do-Hyung , Saadeldin Islam M. , Cho Jongki
Published: August 2021

Molecular approaches have been used to determine metabolic substrates involved in the early embryonic processes to provide adequate culture conditions. To investigate the effect of modified Spirulina maxima pectin nanoparticles (MSmPNPs) on oocyte developmental competence, cumulus–oocyte complexes (COCs) retrieved from pig slaughterhouse ovaries were subjected to various concentrations of MSmPNPs (0, 2.5, 5.0, and 10 µg/mL) during in vitro maturation (IVM). In comparison to the control, MSmPNPs-5.0, and MSmPNPs-10 groups, oocytes treated with 2.5 µg/mL MSmPNPs had significantly increased glutathione (GSH) levels and lower levels of reactive oxygen species (ROS). Following parthenogenetic activation, the MSmPNPs-2.5 group had a considerably higher maturation and cleavage rates, blastocyst development, total cell number, and ratio of inner cell mass/trophectoderm (ICM:TE) cells, when compared with those in the control and all other treated groups. Furthermore, similar findings were reported for the developmental competence of somatic cell nuclear transfer (SCNT)-derived embryos. Additionally, the relative quantification of POU5F1, DPPA2, and NDP52 mRNA transcript levels were significantly higher in the MSmPNPs-2.5 group than in the control and other treated groups. Taken together, the current findings suggest that MSmPNP treatment alleviates oxidative stress and enhances the developmental competence of porcine in vitro matured oocytes after parthenogenetic activation and SCNT.
Deep Learning for Drug Discovery: A Study of Identifying High Efficacy Drug Compounds Using a Cascade Transfer Learning Approach
Authors: Zhuang Dylan , Ibrahim Ali K.
Published: August 2021

In this research, we applied deep learning to rank the effectiveness of candidate drug compounds in combating viral cells, in particular, SARS-Cov-2 viral cells. For this purpose, two different datasets from Recursion Pharmaceuticals, a siRNA image dataset (RxRx1), which were used to build and calibrate our model for feature extraction, and a SARS-CoV-2 dataset (RxRx19a) was used to train our model for ranking efficacy of candidate drug compounds. The SARS-CoV-2 dataset contained healthy, uninfected control or “mock” cells, as well as “active viral” cells (cells infected with COVID-19), which were the two cell types used to train our deep learning model. In addition, it contains viral cells treated with different drug compounds, which were the cells not used to train but test our model. We devised a new cascade transfer learning strategy to construct our model. We first trained a deep learning model, the DenseNet, with the siRNA set, a dataset with characteristics similar to the SARS-CoV-2 dataset, for feature extraction. We then added additional layers, including a SoftMax layer as an output layer, and retrained the model with active viral cells and mock cells from the SARS-CoV-2 dataset. In the test phase, the SoftMax layer outputs probability (equivalently, efficacy) scores which allows us to rank candidate compounds, and to study the performance of each candidate compound statistically. With this approach, we identified several compounds with high efficacy scores which are promising for the therapeutic treatment of COVID-19. The compounds showing the most promise were GS-441524 and then Remdesivir, which overlapped with these reported in the literature and with these drugs that are approved by FDA, or going through clinical trials and preclinical trials. This study shows the potential of deep learning in its ability to identify promising compounds to aid rapid responses to future pandemic outbreaks.
The Role of Fossil Fuel Combustion Metals in PM2.5 Air Pollution Health Associations
Authors: Maciejczyk Polina , Chen Lung-Chi , Thurston George
Published: August 2021

In this review, we elucidate the central role played by fossil fuel combustion in the health-related effects that have been associated with inhalation of ambient fine particulate matter (PM2.5). We especially focus on individual properties and concentrations of metals commonly found in PM air pollution, as well as their sources and their adverse health effects, based on both epidemiologic and toxicological evidence. It is known that transition metals, such as Ni, V, Fe, and Cu, are highly capable of participating in redox reactions that produce oxidative stress. Therefore, particles that are enriched, per unit mass, in these metals, such as those from fossil fuel combustion, can have greater potential to produce health effects than other ambient particulate matter. Moreover, fossil fuel combustion particles also contain varying amounts of sulfur, and the acidic nature of the resulting sulfur compounds in particulate matter (e.g., as ammonium sulfate, ammonium bisulfate, or sulfuric acid) makes transition metals in particles more bioavailable, greatly enhancing the potential of fossil fuel combustion PM2.5 to cause oxidative stress and systemic health effects in the human body. In general, there is a need to further recognize particulate matter air pollution mass as a complex source-driven mixture, in order to more effectively quantify and regulate particle air pollution exposure health risks.
Assessing Heatwaves and Their Association with North African Dust Intrusions in the Algarve (Portugal)
Authors: Fernandes Raquel , Fragoso Marcelo
Published: August 2021

Heatwaves are an extreme meteorological event in which affected populations may also be exposed to deteriorated air quality conditions due to the increase in air pollutant concentrations, such as PM10 (particulate matter < 10 µg/m3). In order to identify heatwaves (1973–2019) in the region of Faro (Algarve) during the hot season (April–September), the Excess Heat Factor (EHF) index was applied. The Mann–Kendall test revealed an upward trend in three heatwave metrics in Faro, and the trend of accumulated heat load (EHF load) was also positive as would be expected, but its signal was not statistically significant. An inventory of North African dust events (2006–2019) was made, and their simultaneous occurrence with heatwaves was assessed, pointing to only 20% of dust events of the Sahara occurring simultaneously with heatwave days. A cluster analysis was conducted on daily geopotential height fields at 850 hPa level over the 2006–2019 period, and four distinct patterns were identified as the most prominent synoptic circulations promoting both heatwave conditions and North African dust over the Algarve.
Ammonia Emission in Poultry Facilities: A Review for Tropical Climate Areas
Authors: Oliveira Matheus Dias , Sousa Fernanda Campos , Saraz Jairo Osorio , Calderano Arele Arlindo , Tinôco Ilda Fátima Ferreira , Carneiro Antônio Policarpo Souza
Published: August 2021

Brazil is the largest broiler meat exporter in the world. This important economic activity generates income in different branches of the production chain. However, the decomposition of residues incorporated in the poultry litter generates several gases, among them ammonia. When emitted from the litter to the air, ammonia can cause several damages to animals and man, in addition to being able to convert into a greenhouse gas. Thus, the aim of this article was to carry out a review of the ammonia emission factors in the production of broilers, the methodologies for measuring, and the inventories of emissions already carried out in several countries. The main chemical processes for generating ammonia in poultry litter have been introduced and some practices that can contribute to the reduction of ammonia emissions have been provided. The PMU, Portable Monitoring Unit, and the SMDAE, Saraz Method for Determination of Ammonia Emissions, with the required adaptations, are methodologies that can be used to quantify the ammonia emissions in hybrid facilities with a natural and artificial ventilation system. An ammonia emission inventory can contribute to the control and monitoring of pollutant emissions and is an important step towards adopting emission reductions. However, quantifying the uncertainties about ammonia emission inventories is still a challenge to be overcome.