bluuebunny/crossref_metadata_2025 huggingface dataset:
Comparison of Full-Endoscopic Visualized Unilateral Laminotomy Bilateral Decompression and Minimally Invasive Transforaminal Lumbar Interbody Fusion for One-Level Lumbar Spinal Stenosis
Authors: Hua Wenbin , Wang Bingjin , Ke Wencan , Wu Xinghuo , Zhang Yukun , Li Shuai , Yang Shuhua , Yang Cao
Published: August 2020

Background: The aim of the present study is to compare the clinical outcomes and postoperative complications of full-endoscopic visualized unilateral laminotomy bilateral decompression (FEV-ULBD) and minimally invasive surgery-transforaminal lumbar interbody fusion (MIS-TLIF) to treat one-level lumbar spinal stenosis (LSS) without degenerative spondylolisthesis or deformity. Methods: A retrospective analysis of 112 consecutive patients of one-level LSS undergoing either FEV-ULBD or MIS-TLIF was performed. Patient demographics, operation time, estimated blood loss, time to ambulation, length of hospitalization, intraoperative and postoperative complications were recorded. The visual analog scale (VAS) score for leg and back pain, the Oswestry Disability Index (ODI) score, and the Macnab criteria were used to evaluate the clinical outcomes. The healthcare cost was also recorded.Results: The operation time, estimated blood loss, time to ambulation and length of hospitalization of FEV-ULBD group were shorter than MIS-TLIF group. The postoperative mean VAS and ODI scores decreased significantly in both groups. According to the modified Macnab criteria, the outcomes rated as excellent/good rate were 90.6% and 93.8% in the two groups. The mean VAS scores, ODI scores and outcomes of the modified Macnab criteria of both groups were of no significant difference. The healthcare cost of FEV-ULBD group was lower than MIS-TLIF group. Two cases of intraoperative epineurium injury were observed in the FEV-ULBD group. One case of cauda equina injury was observed in the FEV-ULBD group. No nerve injury, dural injury or cauda equina syndrome was observed in MIS-TLIF group. However, one case with transient urinary retention, one case with pleural effusion, one case with incision infection and one case with implant dislodgement were observed in MIS-TLIF group. Conclusions: Both FEV-ULBD and MIS-TLIF are safe and effective to treat one-level LSS without degenerative spondylolisthesis or deformity. FEV-ULBD is a more minimally invasive option and of less economic burden compared with MIS-TLIF. Decompression plus instrumented fusion may be not necessary for one-level LSS without degenerative spondylolisthesis or deformity.
Symmetrical Dental Occlusion Blocking - What are the Required Changes of Pressure Distribution to be Clinical Relevant?
Authors: Maurer-Grubinger C , Avaniadi Ioanna , Adjami Frederic , Christian Wolfgang , Doerry Charlotte , Fay Viola , Fisch Vanessa , Gerez Ali , Goecke Julian , Kaya Ugur , Keller Julia , Krüger Dominik , Pflaum Jukia , Porsch Laurin , Wischnewski Christina , Scharnweber Benjamin , Sosnov Polyna , Oremek Gerhard , Groneberg David A. , Ohlendorf Daniela
Published: August 2020

Background: Symmetrical dental occlusion blocking (bicuspid region) is used in dentistry as a quick diagnostic test (“Meersseman test”) to check a possible influence of the temporomandibular system (TMS) on the movement system. Immediate effects between occlusal blocking and rest position on body sway and weight distribution in general and for both genders and for four age decades should be evaluated.Methods: 725 (396f/329m) healthy subjects (neither subjective signs of TMD nor acute/chronic complaints in the musculoskeletal system) volunteered (21 to 60 years) while both genders were divided into four age groups according to decades. A pressure measuring platform was used. The postural control (body sway and weight distribution) was recorded in two dental occlusion conditions (a) in rest position and (b) symmetrical blocking (bicuspid region) by cotton rolls. Results: The frontal sway reduced by 0.67mm (t(724) = -3.9 (p < 0.001)) and the sagittal sway by 0.33mm (t(724) = -3.4 (p < 0.001)). The relative pressure under the forefoot left increased by 0.33% (t(724) = 2.88 (p < 0.001)) and the relative pressure overall under the forefoot increased by 0.67% (t(724) = -3.4 (p < 0.001)). Gender-specific and age-specific reactions cannot be recorded.Discussion: In all subjects the postural sway improved slightly when blocking the occlusion ("Meersseman test") compared to the rest position. There is a slight shift of weight on the forefoot, especially the left forefoot. There exist no age- and gender-specific differences. Clinically relevant differences should apply outside the data between the 1st and 3rd quantile.
Spatial Distribution and Factors Associated with Risky Health Behaviour Among Adult Males Aged 15-59 Years in Ethiopia; Generalized Structural Equation Modelling
Authors: Kebede Sewnet Adem , Weldesenbet Adisu Birhanu , Tusa Biruk Shalmeno
Published: August 2020

Background: Alcohol drinking and tobacco smoking are the largest preventable cause of death and important risk factors for a number of non-communicable diseases and cause premature death and many socioeconomic consequences. Therefore, the present study aimed to assess the spatial distribution of risky health behaviour and its associated factors among adult males in Ethiopia. Methods: Secondary data analysis was conducted among 12,688 men (aged 15–59 years). The distribution of risky health behaviour across the country was observed by ArcGIS software. In SaTScan software, the Bernoulli model was fitted by Kulldorff methods to identify the purely spatial clusters of risky health behaviour. Generalized Structural Equation Model (GSEM) was used to determine factors associated with each domain of risky health behaviour (regular alcohol drinking and tobacco smoking).Results: Risky health behaviour had spatial variation across the country. The primary clusters were located in Tigray, Amhara and north-eastern Benishangul national regional states. Spatial scan statistics identified 118 primary clusters (Log-Likelihood ratio (LLR) =524.8, p < 0.001). Residence, frequency of listening to a radio, occupation and frequency of watching television were significantly associated with drinking alcohol whereas wealth index was associated with tobacco smoking. Age, region, educational status, marital status and religion had association with both domains of risky health behaviour.Conclusion: Risky health behaviour had spatial variation across the country. The national press media in ban of advertising and promotion of alcohol and tobacco should be strengthen. Aggressive health education efforts should be directed toward this high-risk population (Tigray, Amhara and north-eastern Benishangul regional states). Improving risky health behaviour is an important approach to reducing health disparities and for more cost-effective utilization of scarce resources in the public health sector.
Limiting potential COVID-19 contagion in squatting public toilets
Authors: Han Jia , Pan Li , Chen Shuang-lan , Guo Yi-sha , Du Yu-xiang , Wu Xiao-di , Jones Alice YM
Published: August 2020

Background: Since the outbreak of the SARS-CoV-2 virus in December 2019, the COVID-19 pandemic continues to threaten global stability. Transmission of SARS-CoV-2 is mostly by respiratory droplets and direct contact but viral RNA fragments have also been detected in the faecal waste of patients with COVID-19. Cleanliness and effective sanitation of public toilets is a concern, as flushing the toilet is potentially an aerosol generating procedure. When the toilets are of the squatting type and without a cover, there exists a risk of viral contamination through the splashing of toilet water and aerosol generation. Methods: This study reports an online survey of 134 people in China to determine whether the cleanliness of public toilets was a concern to the general population during the COVID-19 pandemic, and whether a squatting toilet was preferred to a seated design.Results: The survey showed that 91% of participants preferred squatting toilets, but that 72% were apprehensive of personal contamination when using public toilets. Over 63% of the respondents had encountered an incidence of water splash and would prefer public toilets to be covered during flushing and 83% of these respondents preferred a foot-controlled device.Conclusions: This survey suggests that consideration should be given to the installation of a simple foot-controlled device to cover public squatting toilets to help restrict potential COVID-19 contamination and to meet hygienic expectations of the public.
Impact of Delirium on Short-term Prognosis in Geriatric Patients with Hip Fracture
Authors: An Shuai , Wu Jingwei , Feng Mingli , Li Zheng , Li Zonghan , Cheng Jingbo , Cao Guanglei , Liu Limin , Lu Shibao
Published: August 2020

Background: To explore the effect of delirium on postoperative early prognosis of geriatric patients with hip fracture. Methods: According to whether postoperative delirium (POD) appeared in elderly patients with hip fracture, all cases were divided into two groups: delirium and non-delirium. General parameters, operative parameters, length of stay (LOS), and perioperative complications were recorded. Recurrent fracture, new stroke, and mortality within 1 year postoperatively were followed up and compared between the two groups. Results: Of 358 cases, 55 (15.4%) developed delirium. Compared with the non-delirium group, the delirium group had longer operative times (P=0.031); increased platelet (P=0.002) and C-reactive protein (P<0.001) levels; and reduced hemoglobin (P<0.001), calcium (P=0.040), albumin (P<0.001), prealbumin (P<0.001), and total cholesterol (P=0.019) levels. Complications occurred to some extent in 196 cases (54.7%). In the delirium group, complication rates of pulmonary infection (P=0.005), hypoalbuminemia (P<0.001), electrolyte disorder (P<0.001), dyspepsia (P=0.027), bedsore (P=0.012) and anemia (P=0.007) were higher. In addition, LOS was significantly longer (15.71 ± 4.72 vs 14.00 ± 5.30 days; P=0.026) in the delirium group. Within 1 year postoperatively, 31 individuals (8.7%) had died, 13 were treated for recurrent fracture, and 42 had a new stroke. In the delirium group, mortality within 1 year postoperatively was higher (27.3% vs 5.3%, P<0.001). Conclusions: The morbidity of POD is high in geriatric patients with hip fractures. There was longer surgery delay, higher complication rate, longer LOS, and increased mortality in the delirium group. Early intervention of POD has important clinical significance.
Community Stakeholder Preferences for Evidence-Based Practice Implementation Strategies in Behavioral Health: A Best-Worst Scaling Choice Experiment
Authors: Williams Nathaniel , Candon Molly , Stewart Rebecca , Byeon Y. Vivian , Bewtra Meenakshi , Buttenheim Alison , Zentgraf Kelly , Comeau Carrie , Shoyinka Sosunmolu , Beidas Rinad S.
Published: August 2020

Background: Community behavioral health clinicians, supervisors, and administrators play an essential role in implementing new psychosocial evidence-based practices (EBP) for patients receiving psychiatric care; however, little is known about these stakeholders’ values and preferences for implementation strategies that support EBP use, nor how best to elicit, quantify, or segment their preferences. This study sought to quantify clinician, supervisor, and administrator preferences for implementation strategies and to identify segments of these stakeholders with distinct preferences using a rigorous choice experiment method called best-worst scaling (BWS). Methods: A total of 240 clinicians, 74 clinical supervisors, and 29 administrators delivering publicly funded behavioral health services in a large metropolitan behavioral health system participated in a best-worst scaling choice experiment. Participants evaluated 14 implementation strategies developed through extensive elicitation and pilot work within the target system. Preference weights were generated for each strategy using hierarchical Bayesian estimation. Latent class analysis identified segments of stakeholders with unique preference profiles. Results: On average, clinicians, supervisors, and administrators preferred two strategies significantly more than all others—compensation for use of EBP per session and compensation for preparation time to use the EBP; two strategies were preferred significantly less than all others—performance feedback via email and performance feedback via leaderboard. However, latent class analysis identified four distinct segments of stakeholders with unique preferences: Segment 1 (n = 121, 35%) strongly preferred financial incentives over all other approaches; Segment 2 (n = 80, 23%) preferred technology-based strategies; Segment 3 (n = 52, 15%) preferred an improved waiting room to enhance client readiness and strongly disliked any type of clinical consultation; Segment 4 (n = 90, 26%) rejected financial incentives and strongly preferred strategies focused on clinical consultation. Conclusions: The presence of four heterogeneous subpopulations within this large group of behavioral health administrators, supervisors, and clinicians suggests optimal implementation may be achieved through targeted strategies derived via elicitation of stakeholder preferences. Best-worst scaling is a feasible, systematic, and rigorous method for eliciting stakeholders’ implementation preferences and identifying subpopulations with unique preferences in behavioral health settings.
The Anti Swing Strategy of Davit System to Unmanned Surface Vehicle at Sea
Authors: Wei Shao-Liang , Wang Haoqi , Feng Zhen-Wei , Zhang Shuai , Cheng Feng-Yu
Published: August 2020

Aiming at the problem that the marine mother ship's davit system cannot accurately and quickly hoist due to wind and waves during the process of recovering the unmanned boat, an anti-swaying gondolas recovery system is designed to realize the automatic hoisting and deployment of the unmanned boat for recovery or deployment. We created a tension-length gondola recovery and deployment system model, which reduced the swing angle of the unmanned boat davit system's hoisting and deployment through adaptive control of the tension and length of the davit ropes, thereby realizing the installation and deployment of unmanned maritime boats at sea. It’s accurate and fast. Simulation experiments show that under the combined action conditions of 10 ° roll angle and 5 ° pitch angle, the deflection range of the gondola system equipped with a deflection device can reach about 60%.
Association of the SLC6A4, SLC6A3, COMT and BDNF Gene Polymorphisms with Suicidal Behavior: A Case Control Study
Authors: Rafikova Ekaterina , Shadrina Maria I. , Slominsky Peter A. , Guekht Alla B. , Ryskov Aleksei P. , Shibalev Dmitry V. , Vasilyev Vasiliy A.
Published: August 2020

BackgroundSuicide causes about 1 million deaths per year. Familial transmission of suicide, as well as the involvement of serotonin and dopamine systems in suicidal behavior, was confirmed by previous studies. We investigated an effect of 11 polymorphisms of 9 genes related to dopamine and serotonin transmission (SLC6A4, HTR1A, HTR2A, HTR1B, SLC6A3, DRD4, DRD2, COMT, and BDNF) on the risk of suicide. MethodsThe study was performed on 100 psychiatric clinic patients with repeated suicide attempts. Genomic DNA was obtained from venous blood. Genotyping was performed using locus-specific PCR. Statistical analysis was carried out using a Pearson Chi-squared test, ORs, with the corresponding 95% CIs and one-way ANOVA.ResultsAssociation of the SLC6A3 40–45 bp VNTR and SLC6A4 rs25531 loci with suicidal behavior was tested for the first time. We have shown that a short allele of SLC6A3 40–45 bp VNTR locus might increase the risk of suicidal attempts (p = 0.009). Contrary to previous research, we have found that the Val-allele of COMT rs4680 is associated with suicide (p = 0.028). T-allele of BDNF rs6264 and A-allele of SLC6A4 rs25531 were associated with suicidal behavior with p = 0.029 and p = 0.047, respectively. ANOVA showed the lack of association between these loci and severity of depressive symptoms, but revealed a possible effect of SLC6A4 40 bp VNTR and HTR1B loci on anxiety symptoms. ConclusionsOur results suggest that allelic variants of SLC6A3, SLC6A4, COMT and BDNF genes might be considered as risk factors for repeated suicide attempts in patients with different mental disorders. Loci SLC6A4 40 bp VNTR and HTR1B rs6296 may affect anxiety symptoms.
The Attenuation of Insulin-Like Growth Factor Signaling May Be Responsible for Relative Reduction in Matrix Synthesis in Degenerated Areas of Osteoarthritc Cartilage
Authors: Tanaka Nobuho , Tsuno Hirotaka , Ohashi Satoru , Iwasawa Mitsuyasu , Furukawa Hiroshi , Kato Tomohiro , Fukui Naoshi
Published: August 2020

Background: In osteoarthritis (OA), cartilage matrix is lost gradually despite enhanced matrix synthesis by chondrocytes. This paradox may be explained, at least partly, by reduced chondrocyte anabolism in degenerated area of OA cartilage. However, to date, it is not known why chondrocyte anabolism is suppressed in those areas. Methods: Cartilage was obtained from control knees and end-stage OA knees in macroscopically preserved areas and degenerated areas, and gene expression was analyzed in respective regions of cartilage using laser capture microdissection and qPCR. For the cartilage protein analysis, cartilage was obtained from preserved areas and degenerated areas of OA knees in pairs, and proteins were extracted using urea buffer. Protein concentrations were determined by Luminex and compared between the areas. Cartilage explants prepared from preserved areas and degenerated areas of OA knees were cultured in the presence or absence of an AKT inhibitor, and the gene expression was evaluated by qPCR. Finally, the expression of SP1 was evaluated in OA and control cartilage, and the significance of Sp1 on the expression of IGF1R and IRS1 was investigated in experiments using primary cultured chondrocytes.Results: Within OA cartilage, the expression of IGF-1, IGF-2, IGF1R and IRS1 was reduced in degenerated areas compared to preserved areas, while the expression of all six IGF-binding protein genes examined was enhanced in the former areas. Consistent results were obtained by a protein analysis. In explant culture, the inhibition of AKT signaling abrogated the abundant matrix gene expression in the preserved areas over the degenerated areas, indicating that suppressed matrix synthesis in degenerated areas may be ascribed, at least partly, to attenuated IGF signaling. Within OA cartilage, the expression of Sp1 was considerably reduced in degenerated areas compared to preserved areas, which correlated well with the expression of IGF1R and IRS1. In experiments using primary cultured chondrocytes, the expression of IGF1R and IRS1 was enhanced by the induction of Sp1 expression and reduced by the suppression of Sp1 expression.Conclusions: The results of this study suggest that attenuated IGF signaling may be responsible, at least partly, for the reduced matrix synthesis in degenerated areas of OA cartilage.
Early Clinical and Radiological outcomes of the New Anatomic Knee System : Minimum 3-year Follow-up.
Authors: Ryu Dong Jin , Jeon Yoon Sang , Shin Byung Ki , Na Yeop , Park Sung Jun , Kim Myung Ku
Published: August 2020

Background: For total knee arthroplasty (TKA), implant design and proper coverage are important for achieving patient satisfaction. An asymmetrical anatomic implant (Persona® knee system, Zimmer Biomet, Warsaw, Indiana) enables accurate coverage and minimal bone cutting. This study aimed to analyze the clinical and radiological outcomes of TKA using an asymmetrical anatomic implant over a minimum 3-year follow-up.Methods: The medical records of 94 patients who underwent 132 TKAs using the Persona® knee system, between April 2015 and January 2016 were reviewed. Pre- and post-operative clinical outcomes (assessed using the International Knee Documentation Committee (IKDC), Western Ontario and McMaster Universities Arthritis Index (WOMAC), and Knee Injury and Osteoarthritis Outcome Score (KOOS) scores, and range of motion (ROM) were reviewed. The mechanical hip-knee-ankle (HKA) axis, component overhang and underhang and presence of a radiolucent line (RLL) were evaluated. Liner thickness were also reviewed.Results: At 3-year follow-up, the mean ROM improved from 108.4° to 130.3°. The mean IKDC subjective, WOMAC and global KOOS score significantly improved from 29.2 to 70.3, from 64.2 to 11.5, and from 66.6 to 21.7, respectively (p<0.0001). The mean mechanical HKA axis indicated the correction of the malalignment (from 9.2° varus to 0.3° varus, p<0.001). The incidence rates of >3-mm bone-to-implant size mismatch were 6.8% and 8.3% for the femoral and tibial components, respectively. RLL was found in seven cases, but they were small (>2 mm) and did not progress. In 36.3% of the knees, the additional liner thickness (11 or 13mm) was used. No case of implant loosening or early failure related to the implant design occurred. Conclusion: TKA using the Persona® knee system improved all the clinical knee scores and ROM without signs of early failure. This knee system also provides more implant size options than the conventional TKA systems and facilitates ligament balancing.
Activation of metabolic and stress responses during subtoxic expression of the type I toxin hok in Erwinia amylovora
Authors: Peng Jingyu , Triplett Lindsay R. , Sundin George
Published: August 2020

Background
Toxin-antitoxin (TA) systems, abundant in prokaryotes, are composed of a toxin gene and its cognate antitoxin. Several toxins are implied to affect the physiological state and stress tolerance of bacteria in a population. However, the molecular targets or regulatory roles of TA systems are largely unknown.
Results
Here, we examined the physiological and transcriptomic changes of Erwinia amylovora cells expressing hok at subtoxic levels that were confirmed to confer no cell death, and at toxic levels that resulted in killing of cells. In both conditions, hok caused membrane rupture and collapse of the proton motive force in a subpopulation of E. amylovora cells. We demonstrated that induction of hok resulted in upregulation of ATP biosynthesis genes, and caused leakage of ATP from cells only at toxic levels. We showed that overexpression of the phage shock protein gene pspA largely reversed the cell death phenotype caused by high levels of hok induction. We also showed that induction of hok at a subtoxic level rendered a greater proportion of stationary phase E. amylovora cells tolerant to the antibiotic streptomycin.
Conclusions
We characterized the molecular mechanism of toxicity by high-level of hok induction and demonstrated that low-level expression of hok primes the stress responses of E. amylovora against further membrane and antibiotic stressors.
Physical mapping and InDel marker development for the restorer gene Rf2 in cytoplasmic male sterile CMS-D8 cotton
Authors: Feng Juanjuan , Zhang Xuexian , Zhang Meng , Guo Liping , QI Tingxiang , Tang Huini , Zhu Haiyong , Wang Hailin , Qiao Xiuqin , Xing Chaozhu , Wu Jianyong
Published: August 2020

Background
Cytoplasmic male sterile (CMS) with cytoplasm from Gossypium Trilobum (D8) fails to produce functional pollen. It is useful for commercial hybrid cotton seed production. The restore line of CMS-D8 containing Rf2 gene can restore the fertility of the corresponding sterile line. This study combined the whole genome resequencing bulked segregant analysis (BSA) with high-throughput SNP genotyping to accelerate the physical mapping of Rf2 locus in CMS-D8 cotton.
Methods
The fertility of backcross population ((sterile line × restorer line) × maintainer line) comprising of 1623 individuals was investigated in the field. The fertile pool (100 plants with fertile phenotypes, F-pool) and the sterile pool (100 plants with sterile phenotypes, S-pool) were constructed for BSA resequencing. Selection of 24 SNPs through high-throughput genotyping and development InDel markers narrow down the candidate interval. The PPR genes and restore line up-regulated genes in the candidate interval were analyzed by RT-PCR.
Results
The fertility investigation results showed that fertile and sterile separation ratio was consistent with 1:1. BSA resequencing technology, high-throughput SNP genotyping, and InDel markers were used to identify Rf2 locus on candidate interval of 1.48 Mb on Chromosome D05. Furthermore, it was quantified in this experiment that InDel markers co-segregated with Rf2 enhanced the selection of the restorer line. The qRT-PCR analysis revealed PPR family gene Gh_D05G3391 located in candidate interval had significantly lower expression than sterile and maintainer lines. In addition, utilization of previous anther RNA-Seq data of CMS-D8 identified that the expression level of Gh_D05G3374 encoding NB-ARC domain-containing disease resistance protein in restorer lines was significantly higher than that in sterile and maintainer lines.
Conclusions
This study not only enabled us to precise locate the restore gene Rf2 but also evaluated the utilization of InDel markers for marker assisted selection in CMS-D8 Rf2 cotton breeding line. The results of this study provide an important foundation for further studies on the mapping and cloning of restorer genes.
Severity of radiation pneumonitis, from clinical, dosimetric and biological features: a pilot study.
Authors: Aso Samantha , Navarro-Martin Arturo , Castillo Richard , Padrones Susana , Castillo Edward , Montes Ana , Ballarin Jose Ignacio Martinez , Cubero Noelia , Lopez Rosa , Rodriguez Laura , Palmero Ramon , Manresa Federico , Guerrero Thomas , Molina Maria
Published: August 2020

Background and objective: Radiation pneumonitis (RP) could be a lethal complication of lung cancer treatment. No reliable predictors of RP severity have been recognized. This prospective pilot study was performed to identify early predictors of high grade lung toxicity and to evaluate clinical, biological or dosimetric features associated with different grades of toxicity.Method: Sixteen patients with non-small cell lung cancer with indication of concurrent chemoradiotherapy using 60Gy/2Gy/fraction starting at cycle one of platinum based chemotherapy were included. Bronchoalveolar lavage (BAL), pulmonary function testing (PFT), and 18F-2-fluoro-2-deoxy-D-glucose positron-emission tomography was performed before radiotherapy (RT), after three weeks of treatment, and two months post-RT. For analysis, patients were grouped by grade (low [G1-G2] vs. high [G3-G5]). The two groups were compared to identify predictors of RP. Protein expression BAL and lung tissue metabolism was evaluated in two patients (RP-G1 vs. RP-G3). Categorical variables such as comorbidities, stages and locations were summarized as percentages. Radiation doses, pulmonary function values and time to RP were summarized by medians with ranges or as means with standard deviation. Longitudinal analysis PFT was performed by a T-test. Results: All 16 patients developed RP, as follows: G1 (5 pts; 31.3%); G2 (5 pts; 31.3%); G3 (5 pts; 31.3%); and G5 (1 pts; 6.1%). Patients with high grade RP presented significant decrease (p=0.02) in diffusing lung capacity for carbon monoxide (DLCO) after three week of RT. No correlation between dosimetric values and RP grades was observed. BAL analysis of the selected patients showed that CXCL-1, CD154, IL-1ra, IL-23, MIF, PAI-1 and IFN-g were overexpressed in the lungs of the RP-G3 patient, even before treatment. The pre-RT SUVmax value in the RP-G3 patient was non-significantly higher than in the patient with RP-G1. Conclusions: RT induces some degree of RP. Our data suggest that decrease in DLCO% is the most sensitive parameter for the early detection of RP. Moreover, we detect biological differences between the two grades of pneumonitis, highlighting the potential value of some cytokines as a prognostic marker for developing high grade lung toxicity. Further multicenter studies with larger sample size are essential to validate these findings.
MiR-489-3p reduced pancreatic cancer proliferation and metastasis by targeting PKM2 and LDHA involving glycolysis
Authors: Zhang Dan , He zhiwei , Shen Yiyi , wang Jie , Liu Tao , Jiang Jianxin
Published: August 2020

Background: Malignant proliferation and chemotherapy resistance are some of the causes of high mortality in pancreatic cancer. MicroRNAs have long been a hot spot in cancer research and are involved in tumor formation and metabolic stress responses. miR-489-3p is involved in inhibiting the growth of many tumors, but its relationship with the growth and metabolism of pancreatic cancer is not clear. Methods：We used RNA in situ hybridization to analyze the differential expression of miR-489-3p in pancreatic cancer tissues and adjacent tissues. The qRT-PCR experiment detected the content of miR-489-3p in pancreatic cancer cell lines and ordinary pancreatic ductal epithelial cells. Then we did experiments in vivo (subcutaneous tumor formation in nude mice) and in vitro (plate cloning, transwell, glycolysis related experiments) experiments to verify that miR-489-3p can continue the invasion and metastasis of pancreatic cancer and glucose metabolism. Furthermore, we confirmed that LDHA and PKM2 are the two targets of miR-489-3p through dual luciferase reporter gene experiments. Finally, several reply experiments were done to verify the regulation mechanism of miR-489-3p.Results: We determined that miR-489-3p is under-expressed in pancreatic cancer tissues by RNA in situ hybridization, and the function acquisition and deletion experiments and glycolysis experiments confirmed that miR-489-3p can inhibit the proliferation and invasion of Glycolysis. We then analyzed the website to find that miR-489-3p can target LDHA and PKM, and then we verified this finding with a luciferase report experiment. Therefore, we proceeded with recovery experiments on LDHA and PKM2, and concluded that miR-489-3p performs its function by targeting LDHA and PKM2. Finally, in vivo experiments confirmed that highly expressed miR-489-3p inhibited the growth of pancreatic cancer. Conclusion: In short, we identified miR-489-3p as a novel chemotherapy target for pancreatic cancer, and its diagnostic value deserves further study.
Internal Fixation of Anterior Acetabular Fractures with a Limited Pararectus Approach and the New Anatomical Plates: Preliminary Results
Authors: Yao Sheng , Chen Kaifang , Zhu Fengzhao , Liu Jia , Wang Yulong , Zeng Lian , Wan Yizhou , Qu Yanzhen , Yang Liang , Yang Xu , Guo Xiaodong
Published: August 2020

Background The surgical treatment of acetabular fracture has adverse outcomes and high risk, and minimally invasive method is a good way to reduce complications and improve hip joint function. This study is to investigate the treatment of certain acetabular fractures primarily involving the anterior column and quadrilateral plate using a limited pararectus approach and the new anatomical plates. Methods A consecutive cohort of 17 patients with anterior displaced acetabular fractures were managed operatively with minimally invasive approach and the new anatomical plates. Ten patients had anterior column fractures, 1 patient had anterior wall fracture, 4 patients had transverse fractures and 2 patients had anterior column with posterior hemi-transverse fractures. The inferior half of the pararectus approach was adopted to open the medial window and to access the anterior column and the quadrilateral plate. The new anatomical plates were used for internal fixation. Residual displacements were assessed on the postoperative CT scans using a standardized digital method. The surgical details, hip functional outcomes, and complications were noted. Results All of the patients were operated using the limited pararectus approach and the new anatomical plates successfully. The mean operative time and blood loss were 90.9 minutes and 334.1 ml, respectively. The average postoperative residual gap and step displacement were 2.9 mm and 0.7 mm, respectively. The radiological outcome was estimated according to the Matta score, ten of the cases were graded anatomical, six were graded imperfect, and one was graded poor. Follow up averaged 15 months. Functional outcomes were excellent for nine, good for six, and fair for two. It was noted that one case of peritoneal injury was repaired intraoperatively.Conclusions The limited pararectus approach with the advantages of more minimally invasive and less complications can be an alternative choice for simple anterior acetabular fractures especially involving the quadrilateral plate. The new anatomical plates can fit with the surface of the acetabulum, which saves the time of remodeling plates during operation and facilitate fracture reduction.
Prediction of Corona Virus Disease 2019 based on SIR model in China
Authors: mu jiangang
Published: August 2020

Background

From the end of 2019 to the beginning of 2020, the Corona Virus Disease 2019 epidemic occurred in Wuhan, China, and quickly spread to the whole country. We used SIR epidemic model to predict the epidemic trend in China.
Materials and Methods

Respectively fitted the Corona Virus Disease 2019 epidemic trend equations in China, Hubei, and Wuhan, predicted future trends, based on the hypothesis of the infectious disease process by the SIR model and the official announcement data of the Chinese Health Commission .
Results

There will be no new cases in the non-Hubei area nationwide after March 8; there will be no new cases in Hubei that non Wuhan after March 12; Wuhan will there be no new cases after March 22 ;
Conclusions

The epidemic will end soon in China, under the prevention and control measures are not relaxed.
Risk Factors for Adolescents' Mental Health During the COVID-19 Pandemic: A Comparison between Wuhan and Other Areas in China
Authors: Chen Shitao , Cheng Zeyuan , Wu Jing
Published: August 2020

Background: The outbreak of Coronavirus Disease is causing considerable acute risk to public health and might have an unanticipated impact on the mental health of children and adolescents in the long run. This study aims to understand whether there is a clinically significant difference in anxiety, depression, and parental rearing style when comparing adolescents from Wuhan and other cities in China. This study also intends to examine whether gender, grade in school, single child status, online learning participation, parents' involvement in COVID-19 related work, and parents being quarantined or infected due to the disease would lead to clinically significant differences in anxiety and depression. Beyond that, this study explored the pathways among the different variables in order to better understand how these factors play a part in impacting adolescents' mental health condition. Results: Results showed that there was a statistically significant difference in anxiety symptoms between participants who were from Wuhan compared to other areas, but not in depressive symptoms. In addition, participants’ grade level, gender, relative being infected, and study online have direct positive predictive value for depressive and anxiety symptoms, whereas location and sibling status have indirect predictive value. Having relatives who participated in COVID-19 related work had positive direct predictive value only toward depression, but not anxiety.Conclusions: This study discovered several risk factors for adolescents’ depression and anxiety during the pandemic. It also called for a greater awareness of Wuhan parents’ mental wellbeing and recommended a systematic approach for mental health prevention and intervention.
Chloroplast Genome Variation and Phylogenetic Relationships of Atractylodes species
Authors: wang yiheng , Wang Sheng , Liu Yanlei , Yuan Qingjun , Sun Jiahui , Guo Lanping
Published: August 2020

Atractylodes DC is the basic original plant of the widely used herbal medicines “Baizhu” and “Cangzhu” and an endemic genus in East Asia. Species within the genus have minor morphological differences, and the universal DNA barcodes cannot clearly distinguish the systemic relationship or identify the species of the genus. In this study, we sequenced the chloroplast genomes of all species of Atractylodes using high-throughput sequencing. The results indicate that the chloroplast genome of Atractylodes has a typical quadripartite structure and ranges from 152,294 bp (A. carlinoides) to 153,261 bp (A. macrocephala) in size. The genome of all species contains 113 genes, including 79 protein-coding genes, 30 transfer RNA genes and four ribosomal RNA genes. Four hotspots, rpl22-rps19-rpl2, psbM-trnD, trnR-trnT(GGU), and trnT(UGU)-trnL, and a total of 42-47 simple sequence repeats (SSR) were identified as the most promising potentially variable makers for species delimitation and population genetic studies. Phylogenetic analyses of the whole chloroplast genomes indicate that Atractylodes is a clade within the tribe Cynareae; Atractylodes species form a monophyly that clearly reflects the relationship within the genus. Our study included investigations of the sequences and structural genomic variations, phylogenetics and mutation dynamics of Atractylodes chloroplast genomes and will facilitate future studies in population genetics, taxonomy and species identification.
Analysis of Magnetic and Non Magnetic Nanoparticles With Newtonian/non-newtonian Base Fluids Over a Nonlinear Stretching Sheet
Authors: Jabeen kanwal , Mushtaq Muhamad , Akram Rana MUhammad
Published: August 2020

Unsteady mixed convectional boundary layer flow of Casson nanofluid having magnetic( Fe3O4) and nonmagnetic(

Al2O3) nanoparticles suspension within two different types of base fluids, water(Newtonian) and

Sodium Alginate(non-Newtonian), which incorporates viscous and ohmic dissipation effects over a permeable

nonlinear stretching sheet with magnetic field effects. Some suitable non-dimensional similarity transformations

are applied to convert the governing PDEs into a system of nonlinear coupled ODEs and then solved

by differential transformation method(DTM) association with Pade-approximation. To validate the present

results for limited cases, a comparison was made with previously existing literature and found to be in well

agreement. The impact of radiation, internal heat sink/ source, viscous and ohmic dissipation was discussed for

magnetic and non-magnetic nanofluid categories by tabular and graphical demonstrations. We have presented

the tabular results of various emerging parameters to discuss the nature of skin friction, Nusselt and Sherwood

numbers. It was also observed that performance of non-Newtonian(Sodium Alginate) fluid in heat and mass

transfer is better than Newtonian(water) based fluid but no major difference was seen in heat and mass transfer

when comparison was made with Magnetic and Non-magnetic nanoparticles
Prediction of intraperitoneal adhesions using striae gravidarum and scar characteristics in women undergoing repeated cesarean sections
Authors: Elprince Mohamed , Taha Omima Tharwat , khamees rasha , atwa khaled , gadallah Ahmed , al-okda noha , aal radwa abdel , ibrahim Mohamed , Aboelroose Ahmed , ashour osama , elgedawy asmaa , elbahie amira , ghoneim hanan , ahmed amal
Published: August 2020

Background: The current fact of increasing rates of cesarean deliveries is a catastrophe. Recurrent cesareans result in intraperitoneal adhesions that would lead to maternal morbidity during delivery. Great efforts are directed towards the prediction of intraperitoneal adhesions to provide the best care for laboring women. The aim of the current study was to evaluate the role of abdominal striae and cesarean scar characters in the prediction of intraperitoneal adhesions.Methods: This was a case- control study conducted in the emergency ward of the obstetrics and gynecology department of a tertiary hospital from June to December 2019. The study was carried on patients admitted to the ward fulfilling particular inclusion and exclusion criteria. The study included two groups, group one was assessed for the presence of striae, and the degree of intraperitoneal adhesions was evaluated during the current cesarean section. Group two included patients without evidence of abdominal striae. They were evaluated for the severity of adhesions also after evaluation of the previous scar. Evaluation of the striae was done using Davey's scoring system. The scar was assessed using the Vancouver Scar Scale. The modified Nair's scoring system was used to evaluate intraperitoneal adhesions.Results: The study group included 203 women, while the control group included 205 women. There were significant differences in the demographic characters of the recruited patients (p-value 0.001 for almost all variables). The mean Davey score in those with mild, moderate, and severe striae was 1.82 ± 0.39, 3.57 ± 0.5, and 6.73 ± 0.94, respectively (p-value <0.001). Higher scores for the parameters of the Vancouver scale were present in patients with severe striae (1.69 ± 1.01, 1.73 ± 0.57, 2.67 ± 1.23, and 1.35 ± 1.06 for scar vascularity, pigmentation, pliability, and height respectively with a p-value of <0.001 each). Thick intraperitoneal adhesions were noted significantly in women with severe striae [21 (43.75%), p-value <0.001)]. The Davey's and Vancouver scores showed highly significant predictive performance in the prediction of intraperitoneal adhesions (p-value < 0.001).Conclusion: Abdominal striae and cesarean scar were significant predictors for intraperitoneal adhesions.
Influence of Depression on Physical and Mental Health in Patients with Angina
Authors: Alharbi Raed Abdullah
Published: August 2020

Purpose: Angina is well known as chest pain caused by insufficient flow of oxygen-rich blood to the cardiac muscle. Although there are several studies showing an association between depression and angina, none of these studies investigated the influence of depression on the physical and mental health of adult patients with angina, which is the overall goal of this study.Methods: This study used a publicly open data source from the Behavioral Risk Factor Surveillance System (BRFSS), 2017, in the United States. The BRFSS questionnaire relating to health, behaviors, and social life was administered to the United States residents through a telephone interview. There were 450,013 participants in this survey. Different statistical methods, including descriptive, inferential, and predictive methods, were conducted through the Statistical Analysis System (version 9.4) to accomplish the goal of this study. Results: Of the 450,013 participants in the BRFSS 2017, 25,389 were adult patients diagnosed with angina. Of these, 22,875 have depression. This study showed that depression had a statistically significant influence on physical and mental health in adult patients with angina.Conclusion: Depression has a significant influence on physical and mental health of adult patients with angina. Depression increased the physical health problems by 61.95% and mental health problems by 33.15% in adult patients with angina.
Predictors of Psychiatric Disorders or uses of Psychotropic Drugs in Patients with Confirmed COVID-19 During Treatment in a Hospital : A Retrospective Chart Review Running title : Mental health of COVID-19 patients
Authors: Kim Jang Rae , Han Myung Hwa , Lee So Hee
Published: August 2020

BackgroundThis study aims to examine the psychiatric diagnoses, psychotropic drug use, and related factors in patients with coronavirus disease-19 (COVID-19) during their acute treatment phase.MethodsWe performed a retrospective chart review of patients admitted to the isolation ward at a nationally designated hospital during the COVID-19 outbreak. All the COVID-19 patients admitted to the hospital were assigned to psychiatrists by consultation in order to monitor their mental health during their hospital stay. We analyzed the medical records and self-reported psychological tests of 80 out of 112 patients.ResultsOn admission, 16.3% of the subjects had self-reported depression and 6.3% had post-traumatic stress disorder (PTSD). Twenty-five patients (31.3%) had current diagnoses of mental disorders. These disorders consisted of nonorganic insomnia, acute stress reaction, panic disorder, and depressive episodes. Nineteen patients (23.8%) were prescribed psychotropic drugs, including trazodone, alprazolam, clonazepam, and zolpidem. Factors associated with these psychiatric diagnoses included self-reported PTSD on the day of admission and having a spouse. Previous psychiatric history and age of 40 years or more were associated with the use of psychotropic drugs.ConclusionMental health services rendered to quarantined COVID-19 patients undergoing an acute treatment phase and self-reported PTSD on the day of admission might be useful in predicting mental health problems during hospital days.
A Research of STEAP1 on the Biological Behavior of Gastric Cancer
Authors: Zhang Zhe , Xu Huimian
Published: August 2020

Introduction:Six-Transmembrane Epithelial Antigene of the Prostate 1 (STEAP1) is associated with the occurrence and development of cancer. This study aimed to clarify the role of STEAP1 in gastric cancer tumor growth and metastasis, as well as its molecular mechanism of action. Methods：Statistical methods were used for clinical data analysis. Protein expression was detected using immunohistochemistry(IHC). The mRNA and protein expression in the cell cultures were detected using reverse transcription-polymerase chain reaction(RT-PCR) and western blot analysis. Overexpression and silencing models were constructed using plasmid and lentivirus transfection. To detect cell proliferation in vitro, Cell Counting Kit-8(CCK-8), flow cytometry, and colony formation assays were used; transwell and wound healing assays were used to detect cell migration and invasion; For in vivo experiments, nude BALB/c mice were used for detecting subcutaneous tumorigenesis and intraperitoneal implantation. Results：We found STEAP1 was overexpressed in gastric cancer tissues and cell lines. Single factor and Cox analyses showed that STEAP1 gene expression level correlated with poor prognosis. Upregulation of STEAP1 increased cell proliferation, migration, and invasion, which decreased after STEAP1 was knocked down. These changes were achieved via the activation of the AKT/FoxO1 pathway and epithelial-mesenchymal transformation (EMT). The in vivo animal experiments showed that STEAP1 knock down, resulted in a decrease in the subcutaneous tumor and peritoneal tumor formation.Conclusions：STEAP1 was overexpressed in gastric cancer and closely connected with OS. STEAP1 can regulate the cell cycle via the Akt/FoxO1 pathway to influence cell proliferation. STEAP1 may affect cell migration and invasion via EMT induction.