bluuebunny/crossref_metadata_2025 huggingface dataset:
The Role of Emission Sources and Atmospheric Sink in the Seasonal Cycle of CH4 and δ13-CH4: Analysis Based on the Atmospheric Chemistry Transport Model TM5
Authors: Kangasaho Vilma , Tsuruta Aki , Backman Leif , Mäkinen Pyry , Houweling Sander , Segers Arjo , Krol Maarten , Dlugokencky Edward J. , Michel Sylvia , White James W. C. , Aalto Tuula
Published: May 2022

This study investigates the contribution of different CH4 sources to the seasonal cycle of δ13C during 2000–2012 by using the TM5 atmospheric transport model, including spatially varying information on isotopic signatures. The TM5 model is able to produce the background seasonality of δ13C, but the discrepancies compared to the observations arise from incomplete representation of the emissions and their source-specific signatures. Seasonal cycles of δ13C are found to be an inverse of CH4 cycles in general, but the anti-correlations between CH4 and δ13C are imperfect and experience a large variation (p=−0.35 to −0.91) north of 30° S. We found that wetland emissions are an important driver in the δ13C seasonal cycle in the Northern Hemisphere and Tropics, and in the Southern Hemisphere Tropics, emissions from fires contribute to the enrichment of δ13C in July–October. The comparisons to the observations from 18 stations globally showed that the seasonal cycle of EFMM emissions in the EDGAR v5.0 inventory is more realistic than in v4.3.2. At northern stations (north of 55° N), modeled δ13C amplitudes are generally smaller by 12–68%, mainly because the model could not reproduce the strong depletion in autumn. This indicates that the CH4 emission magnitude and seasonal cycle of wetlands may need to be revised. In addition, results from stations in northern latitudes (19–40° N) indicate that the proportion of biogenic to fossil-based emissions may need to be revised, such that a larger portion of fossil-based emissions is needed during summer.
Associations of Human Cognitive Abilities with Elevated Carbon Dioxide Concentrations in an Enclosed Chamber
Authors: Cao Xiaodong , Li Pei , Zhang Jie , Pang Liping
Published: May 2022

Fifteen participants were exposed in an enclosed environmental chamber to investigate the effects of elevated carbon dioxide (CO2) concentration on their cognitive abilities. Three CO2 conditions (1500, 3500, and 5000 ppm) were achieved by constant air supply and additional ultrapure CO2. All participants received the same exposure under each condition, during which they performed six cognitive tests evaluating human perception, attention, short-term working memory, risky decision-making, and executive ability. Generalized additive mixed effects model (GAMM) results showed no statistically significant differences in performance on the reaction time (RT) tests, the speed perception test, and the 2-back test. This suggests that elevated CO2 concentrations below 5000 ppm did not affect participants’ perception and short-term working memory. However, a significant increase in response time was observed in the visual search (VS) test, the balloon simulation risk test (BART), and the Stroop test at 5000 ppm compared to lower exposure concentrations. The slower responses reflected the detrimental effects of elevated CO2 concentrations on visual attention, risky decision-making, and executive ability. The findings suggest that the control level of CO2 concentrations should be tighter in enclosed workplaces where rapid response and operational safety are required.
Bounds for Quotients of Inverse Trigonometric and Inverse Hyperbolic Functions
Authors: Thool Sumedh B. , Bagul Yogesh J. , Dhaigude Ramkrishna M. , Chesneau Christophe
Published: May 2022

We establish new simple bounds for the quotients of inverse trigonometric and inverse hyperbolic functions such as sin−1xsinh−1x and tanh−1xtan−1x. The main results provide polynomial bounds using even quadratic functions and exponential bounds under the form eax2. Graph validation is also performed.
Reconstruction of the rRNA Sequences of LUCA, with Bioinformatic Implication of the Local Similarities Shared by Them
Authors: Men Yu , Lu Guoliang , Wang Yanhui , Lin Jinzhong , Xie Qiang
Published: May 2022

The theory of the RNA world, especially with the catalytic capability of RNA, provides a reasonable framework explaining the evolution of molecular genetics system before the scenario of the central dogma. However, it remains a challenge to deduce the origin mechanism of rRNAs. Here we reconstructed the phylogenetic relationships of archaea and bacteria with bootstrap values of most nodes, especially the deep ones, higher than 90%. Based on the well-resolved tree, the full lengths of 16S, 5S, and 23S rRNA sequences of the last universal common ancestor (LUCA) were reconstructed for the first time. The potential similarities shared by the three ancestral rRNA sequences were further explored by searching for repeat short fragments in the level of purine–pyrimidine (RY) with certain lengths and arrangements. With the lengths ranging from 2 to 14, functional short fragments could be found in the three RNAs. As a representative, a set with a total of 75 short fragments of 11 nucleotides in length can recover all types of the known functional sites of ribosomes in a most concise manner. The 75 short fragments cluster around the functional center of the ribosome, among which 18 of them are highly conserved across five or six kingdoms and still contain all types of known functional sites except one. Alternatively, according to the strategy using the level of AUGC instead of RY, a similar pattern can be recovered. Such results indicate the local similarities shared by 16S, 5S, and 23S rRNAs and thus suggest a possible general mechanism in the formation of the LUCA rRNAs.
Aldehydes, Aldehyde Metabolism, and the ALDH2 Consortium
Authors: Rwere Freeborn , Yu Xuan , Chen Che-Hong , Gross Eric R.
Published: May 2022

The discovery of aldehydes dates back to 1774 when Carl Wilhelm Scheele synthesized acetaldehyde [...]
The Predictive Value of NLR, MLR, and PLR in the Outcome of End-Stage Kidney Disease Patients
Authors: Mureșan Adrian Vasile , Russu Eliza , Arbănași Emil Marian , Kaller Réka , Hosu Ioan , Arbănași Eliza Mihaela , Voidăzan Septimiu Toader
Published: May 2022

Background: Chronic kidney disease (CKD) is a global public health problem with a high mortality rate and a rapid progression to end-stage kidney disease (ESKD). Recently, the role of inflammation and the correlation between inflammatory markers and CKD progression have been studied. This study aimed to analyze the predictive value of the neutrophil–lymphocyte ratio (NLR), monocyte-to-lymphocyte ratio (MLR), and platelet-to-lymphocyte ratio (PLR) in assessing the outcome of ESKD patients. Methods: A retrospective study which included all patients admitted in the Department of Nephrology of the County Emergency Clinical Hospital, Târgu-Mureș, Romania, between January 2016 and December 2019, diagnosed with ESKD. Results: Mortality at 30 days was clearly higher in the case of the patients in the high-NLR groups (40.12% vs. 1.97%; p < 0.0001), high-MLR (32.35% vs. 4.81%; p < 0.0001), and respectively high-PLR (25.54% vs. 7.94%; p < 0.0001). There was also a significant increase in the number of hospital days and the average number of dialysis sessions in patients with high-NLR (p < 0.0001), high-MLR (p < 0.0001), and high-PLR (p < 0.0001). The multivariate analysis showed that a high baseline value for NLR (p < 0.0001), MLR (p < 0.0001), and PLR (p < 0.0001) was an independent predictor of 30-day mortality for all recruited patients. Conclusions: Our findings established that NLR, MLR, and PLR determined at hospital admission had a strong predictive capacity of all-cause 30-day mortality in ESKD patients who required RRT for at least 6 months. Elevated values of the ratios were also associated with longer hospital stays and more dialysis sessions per patient.
Effective Radiosensitization of Bladder Cancer Cells by Pharmacological Inhibition of DNA-PK and ATR
Authors: Chughtai Ahmed Ali , Pannhausen Julia , Dinger Pia , Wirtz Julia , Knüchel Ruth , Gaisa Nadine T. , Eble Michael J. , Rose Michael
Published: May 2022

This study aims at analyzing the impact of the pharmacological inhibition of DNA damage response (DDR) targets (DNA-PK and ATR) on radiosensitization of bladder cancer cell lines of different molecular/histological subtypes. Applying DNA-PK (AZD7648) and ATR (Ceralasertib) inhibitors on SCaBER, J82 and VMCUB-1 bladder cancer cell lines, we revealed sensitization upon ionizing radiation (IR), i.e., the IC50 for each drug shifted to a lower drug concentration with increased IR doses. In line with this, drug exposure retarded DNA repair after IR-induced DNA damage visualized by a neutral comet assay. Western blot analyses confirmed specific inhibition of targeted DDR pathways in the analyzed bladder cancer cell lines, i.e., drugs blocked DNA-PK phosphorylation at Ser2056 and the ATR downstream mediator CHK1 at Ser317. Interestingly, clonogenic survival assays indicated a cell-line-dependent synergism of combined DDR inhibition upon IR. Calculating combined index (CI) values, with and without IR, according to the Chou–Talalay method, confirmed drug- and IR-dose-specific synergistic CI values. Thus, we provide functional evidence that DNA-PK and ATR inhibitors specifically target corresponding DDR pathways retarding the DNA repair process at nano-molar concentrations. This, in turn, leads to a strong radiosensitizing effect and impairs the survival of bladder cancer cells.
Mesenchymal Stromal Cells in Osteoarthritis: Evidence for Structural Benefit and Cartilage Repair
Authors: Song Yujie , Jorgensen Christian
Published: May 2022

Osteoarthritis (OA) presents a major clinical challenge to rheumatologists and orthopedists due to the lack of available drugs reducing structural degradation. Mesenchymal stromal cells (MSCs) may represent new therapeutic approaches in cartilage regeneration. In this review, we highlight the latest knowledge on the biological properties of MSC, such as their chondrogenic and immunomodulatory potential, and we give a brief overview of the effects of MSCs in preclinical and clinical studies of OA treatment and also compare different MSC sources, with the adipose tissue-derived MSCs being promising. Then, we focus on their structural benefit in treating OA and summarize the current evidence for the assessment of cartilage in OA according to magnetic resonance imaging (MRI) and second-look arthroscopy after MSC therapy. Finally, this review provides a brief perspective on enhancing the activity of MSCs.
Computer-Aided Detection of Fiducial Points in Seismocardiography through Dynamic Time Warping
Authors: Chen Chien-Hung , Lin Wen-Yen , Lee Ming-Yih
Published: May 2022

Accelerometer-based devices have been employed in seismocardiography fiducial point detection with the aid of quasi-synchronous alignment between echocardiography images and seismocardiogram signals. However, signal misalignments have been observed, due to the heartbeat cycle length variation. This paper not only analyzes the misalignments and detection errors but also proposes to mitigate the issues by introducing reference signals and adynamic time warping (DTW) algorithm. Two diagnostic parameters, the ratio of pre-ejection period to left ventricular ejection time (PEP/LVET) and the Tei index, were examined with two statistical verification approaches: (1) the coefficient of determination (R2) of the parameters versus the left ventricular ejection fraction (LVEF) assessments, and (2) the receiver operating characteristic (ROC) classification to distinguish the heart failure patients with reduced ejection fraction (HFrEF). Favorable R2 values were obtained, R2 = 0.768 for PEP/LVET versus LVEF and R2 = 0.86 for Tei index versus LVEF. The areas under the ROC curve indicate the parameters that are good predictors to identify HFrEF patients, with an accuracy of more than 92%. The proof-of-concept experiments exhibited the effectiveness of the DTW-based quasi-synchronous alignment in seismocardiography fiducial point detection. The proposed approach may enable the standardization of the fiducial point detection and the signal template generation. Meanwhile, the program-generated annotation data may serve as the labeled training set for the supervised machine learning.
Olfactory Ensheathing Cells Alleviate Facial Pain in Rats with Trigeminal Neuralgia by Inhibiting the Expression of P2X7 Receptor
Authors: Lu Jiafeng , Yang Baolin , Liao Jiayi , Chen Baokang , Lu Mingxin , Zhang Wenjun , Zeng Jingnan , Cheng Hui , Liu Zengxu
Published: May 2022

Trigeminal neuralgia (TN) is a common facial neuropathic pain that is mainly characterized by spontaneous or induced needling or electric shock pain in the innervation area of the trigeminal nerve. It is also referred to as “the cancer that never dies”. The olfactory ensheathing cell (OEC) is a special glial cell in the nervous system that has a strong supportive function in nerve regeneration. Cell transplantation therapy is a useful treatment modality that we believe can be applied in TN management. In this study, OECs were transplanted into the ligation site of the infraorbital nerve of rats. We found that after the OEC transplantation, mechanical pain threshold in the face of the rats was significantly increased. Western blotting, immunofluorescence assay, and reverse transcription-quantitative polymerase chain reaction were performed on the trigeminal ganglia (TG) of model rats. The results revealed a decrease in the expression of P2X7 receptor (P2X7R) in the trigeminal ganglia. Our findings show that OEC transplantation has a good therapeutic effect on TN in rats, and that can reduce the expression of P2X7R in trigeminal ganglia. Therefore, we think that OEC transplantation may be a suitable treatment for TN.
Experimental Study and Numerical Simulation for the Seismic Performance of an Innovative Connection between a Flat CFST Column and an H Beam
Authors: Deng En-Feng , Wang Yu-Han , Liu Zhe , Song Yong-Ji , Wang Zhen , Cao Dian-Bin
Published: May 2022

A concrete-filled steel tube (CFST) column permits convenient and fast construction, and its use for high-rise buildings is increasing. Meanwhile, the CFST structure has great potential for use in residence buildings, owing to its smooth evaluation. A connection for a flat CFST column has also attracted increasing attention from scholars. An innovative connection between a flat CFST column and an H beam was proposed and cyclically tested in this paper. The flat CFST column, with a width that is equal to the thickness of the partition wall, was adopted to avoid the protrusion of the column into the corner of the room. The configuration of the innovative connection was introduced, and three full-scale specimens, considering different relative positions of the beam and column, were tested under cyclic load to failure. The seismic performance, including the failure mode, ductility, etc., were revealed and evaluated. It was indicated that the plastic hinge of the connection was prominently removed outward, due to the reinforced short beam and the interior-diaphragm, verifying the reliability of the innovative connection. Furthermore, an elaborated finite element model was developed, and the results of the finite element simulation were compared with the experimental simulations. This comparison confirmed the reasonability of the developed finite element model.
Effects of Floor System on Progressive Collapse Behavior of RC Frame Sub-Assemblages
Authors: Elkholy Said , Shehada Ahmad , El-Ariss Bilal
Published: May 2022

The ability to predict the resistance of reinforced concrete (RC) structures to progressive collapse as a result of an interior column removal has become a need in structural design. In general, three resistance mechanisms characterize the structure resistance to progressive collapse, flexural action, compressive arch action, and tension catenary action. The objective of this study is to investigate the effects of floor system configurations on the progressive collapse-resistance of RC frame sub-assemblages and the amount of energy dissipated in each resistance mechanism. This investigation employs a fiber element-based modeling technique to present findings into the effects of beam size and reinforcement details on the progressive collapse-resistance and energy dissipation of RC beam-column sub-assemblages with four equal spans. Three different span lengths of 5, 6, and 7 m were considered. A total of 38 floor system designs for gravity loads were performed in accordance with the ACI 318-14 design code. The modeling technique employed in this study was validated and utilized by the authors in previously published works. The study shows that beam size and the presence of slab are critical as they significantly affect the energy dissipation and progressive collapse-resistance and failure pattern of the sub-assemblage frames. Moreover, the presence of a slab was found to increase the energy dissipation by around 28%.
Development of an Ultra-High-Performance Fibre-Reinforced Concrete (UHPFRC) Manufacturable at Ambient Temperature
Authors: Tamataki Koji , Ito Tomoaki , Fujino Yutaka , Yoshitake Isamu
Published: May 2022

Ultra-high-performance fibre-reinforced concrete (UHPFRC) manufacturing typically requires heat curing. UHPFRC production at a ready-mixed concrete (RMC) plant is often difficult because specific equipment is required for heat curing. Concerns associated with ultra-high-performance concrete (UHPC) construction include the energy costs and environmental impacts of the heat curing and transportation from the factory to the construction site. Few studies have been conducted on the manufacturing of UHPFRC under standard curing conditions. The strength properties of UHPFRC manufactured under standard curing are typically poorer than those of UHPFRC manufactured under heat curing. The materials and mixture proportions required for the UHPFRC manufacturable under ambient temperature conditions were investigated. Five types of cement and four types of powder materials were tested, as well as the fine aggregate needed to achieve proper fluidity. This paper reports that the cement having low C3A and high C3S is suitable for the UHPFRC manufacturable at ambient temperatures; the allowable volume of fine aggregate was 600 kg/m3 for the UHPFRC having a proper dispersion of steel fibres; the highest water-binder ratio (W/B) of 21% was found for the UHPFRC cured under ambient temperature.
Interplay of Message Frame and Reference Point on Recycled Water Acceptance in Green Community: Evidence from an Eye-Tracking Experiment
Authors: Fu Hanliang , Xue Pengdong , Wu Zhifang , Zhu Hong , Niu Jiachen , Lai Yu , Hou Caixia
Published: May 2022

Public rejection of recycled water hinders the application of recycled water use projects in green communities. An effective information outreach strategy could help to overcome this obstacle. This study used message frames and reference points as control variables to design experimental materials and conduct eye-movement experiments to determine the effect of different information promotion strategies. The results of the study show that: (1) compared with the loss frame, the gain-framed messages are more effective; (2) self-referencing messages are more suitable for recycled water use promotion than other-referencing messages; (3) message frame (gain vs. loss) and reference point (self vs. others) have an interactive effect on the public’s information cognitive behavior; (4) the average duration of fixations for advertising message plays an intermediary role in the path of message frame and reference point jointly influencing the public acceptance. This study provides managerial implications for determining information dissemination strategies for applying recycled water-use projects in green communities.
Addition of Standard Enzalutamide Medication Shows Synergistic Effects on Response to [177Lu]Lu-PSMA-617 Radioligand Therapy in mCRPC Patients with Imminent Treatment Failure—Preliminary Evidence of Pilot Experience
Authors: Rosar Florian , Bader Hanna , Bartholomä Mark , Maus Stephan , Burgard Caroline , Linxweiler Johannes , Khreish Fadi , Ezziddin Samer
Published: May 2022

Well-received strong efficacy of prostate-specific membrane antigen (PSMA)-targeted radioligand therapy (RLT) does not prevent patients from either early or eventual disease progression under this treatment. In this study, we investigated co-medication with enzalutamide as a potential re-sensitizer for PSMA-RLT in patients with imminent treatment failure on standard 177Lu-based PSMA-RLT. Ten mCRPC patients who exhibited an insufficient response to conventional [177Lu]Lu-PSMA-617 RLT received oral medication of enzalutamide 160 mg/d as an adjunct to continued PSMA-RLT. Prostate-specific antigen (PSA) and standard toxicity screening lab work-up were performed to assess the treatment efficacy and safety in these individuals. The mean PSA increase under PSMA-RLT before starting the re-sensitizing procedure was 22.4 ± 26.5%. After the introduction of enzalutamide medication, all patients experienced a PSA decrease, –43.4 ± 20.0% and –48.2 ± 39.0%, after one and two cycles of enzalutamide-augmented PSMA-RLT, respectively. A total of 70% of patients (7/10) experienced partial remission, with a median best PSA response of –62%. Moreover, 5/6 enzalutamide-naïve patients and 2/4 patients who had previously failed enzalutamide exhibited a partial remission. There was no relevant enzalutamide-induced toxicity observed in this small cohort. This pilot experience suggests the synergistic potential of adding enzalutamide to PSMA-RLT derived from the intra-individual comparison of 177Lu-based PSMA-RLT ± enzalutamide.
Low-Temperature Selective NO Reduction by CO over Copper-Manganese Oxide Spinels
Authors: Fan Fenglan , Wang Lingjuan , Wang Lei , Liu Jinyu , Wang Minghui
Published: May 2022

Selective catalytic reduction of NO with CO (CO-SCR) has been suggested as an attractive and promising technology for removing NO and CO simultaneously from flue gas. Manganese-copper spinels are a promising CO−SCR material because of the high stability and redox properties of the spinel structure. Here, we synthesized CuxMn3−xO4 spinel by a citrate-based modified pechini method combining CuO and MnOx, controlling the molar Cu/Mn concentrations. All the samples were characterized by SEM, EDX, XRD, TEM, H2−TPR, XPS and nitrogen adsorption measurements. The Cu1.5Mn1.5O4 catalyst exhibits 100% NO conversion and 53.3% CO conversion at 200 °C. The CuxMn3−xO4 catalyst with Cu-O-Mn structure has a high content of high valence Mn, and the high mass transfer characteristics of the foam-like structure together promoted the reaction performance. The CO-SCR catalytic performance of Cu was related to the spinel structure with the high ratio of Mn4+/Mn, the synergistic effect between the two kinds of metal oxides and the multistage porous structure.
TLR2 and TLR4 Modulate Mouse Ileal Motility by the Interaction with Muscarinic and Nicotinic Receptors
Authors: Layunta Elena , Forcén Raquel , Grasa Laura
Published: May 2022

Irritable bowel syndrome (IBS) is a chronic functional bowel disorder characterized by intestinal dysmotility. Changes in intestinal microbiota (dysbiosis) can lead to alterations in neuro-muscular functions in the gut. Toll-like receptors (TLRs) 2 and 4 recognize intestinal bacteria and are involved in the motor response induced by gastrointestinal (GI) neurotransmitters. Acetylcholine (ACh) is a well-known neurotransmitter involved in the regulation of GI motility. This study aimed to evaluate the role of TLR2 and TLR4 in the intestinal motor-response induced by ACh in the mouse ileum, as well as the expression and function of the muscarinic and nicotinic ACh receptors. Muscle contractility studies showed that the contractions induced by ACh were significantly lower in TLR2−/− and TLR4−/− with respect to WT mice. In WT mice, the contractions induced by ACh were reduced in the presence of AF-DX AF-DX 116 (a muscarinic ACh receptor (mAChR) M2 antagonist), 4-DAMP (a mAChR M3 antagonist), mecamylamine (a nicotinic AChR receptor (nAChR) α3β4 antagonist) and α-bungarotoxin (a nAChR α7 antagonist). In TLR2−/− mice, the contractions induced by ACh were increased by AF-DX 116 and mecamylamine. In TLR4−/− mice, the contractions induced by ACh were reduced by α-bungarotoxin and 4-DAMP. The mRNA and protein expressions of M3 and α3 receptors were diminished in the ileum from TLR2−/− and TLR4−/− with respect to WT mice. However, the levels of mRNA and protein of β4 were diminished only in TLR4−/− but not in TLR2−/− mice. In conclusion, our results show that TLR2 and TLR4 modulates the motor responses to ACh in the mouse ileum. TLR2 acts on muscarinic M2 and M3 and nicotinic α3β4 ACh receptors, while TLR4 acts on muscarinic M3 and nicotinic α3β4 and α7 ACh receptors.
Effect of Sperm Selection by Magnetic-Activated Cell Sorting in D-IUI: A Randomized Control Trial
Authors: González-Ravina Cristina , Santamaría-López Esther , Pacheco Alberto , Ramos Julia , Carranza Francisco , Murria Lucía , Ortiz-Vallecillo Ana , Fernández-Sánchez Manuel
Published: May 2022

Clinical outcome in assisted reproduction techniques (ARTs) is mainly influenced by the quality of gametes used. It is known that a high percentage of sperm DNA fragmentation (DNAf) decreases the success of ART clinical results. Therefore, techniques such as magnetic-activated cell sorting (MACS) help to improve results in cases of patients with a high percentage of DNAf. Cryopreservation of sperm in donor intrauterine insemination (D-IUI) treatments increases sperm DNAf, so patients using these sperm samples can benefit from using this technique. This prospective randomized national multicenter study analyzed clinical outcomes of 181 D-IUI treatments. MACS was performed after density gradient centrifugation (DGC) in 90 thawed semen donor samples (MACSG), whereas only DGC was performed in 91 thawed semen donor samples (CG). To our knowledge, this is the first study analyzing the effect of MACS on D-IUI cycles. Our results show no significant differences in gestation, live birth, or miscarriage rates between the two groups. We believe that further studies with a larger sample size are needed to evaluate the application of MACS in combination with standard IUI donor sperm preparations in fertility clinics.
Progress in Pulmonary Vein Stenosis: Lessons from Success in Treating Pulmonary Arterial Hypertension
Authors: Jenkins Kathy J. , Fineman Jeffrey R.
Published: May 2022

Pulmonary vein stenosis (PVS) is a rare and poorly understood condition that can be classified as primary, acquired, status-post surgical repair of PVS, and/or associated with developmental lung disease. Immunohistochemical studies demonstrate that obstruction of the large (extrapulmonary) pulmonary veins is associated with the neointimal proliferation of myofibroblasts. This rare disorder is likely multifactorial with a spectrum of pathobiology. Treatments have been historically surgical, with an increasing repetitive interventional approach. Understanding the biology of these disorders is in its infancy; thus, medical management has lagged behind. Throughout medical history, an increased understanding of the underlying biology of a disorder has led to significant improvements in care and outcomes. One example is the treatment of pulmonary arterial hypertension (PAH). PAH shares several common themes with PVS. These include the spectrum of disease and biological alterations, such as vascular remodeling and vasoconstriction. Over the past two decades, an exponential increase in the understanding of the pathobiology of PAH has led to a dramatic increase in medical therapies that have changed the landscape of the disease. We believe that a similar approach to PVS can generate novel medical therapeutic targets that will markedly improve the outcome of these vulnerable patients.
The Performance Comparison of Socioeconomic and Behavioural Factors as Predictors of Higher Blood Lead Levels of 0–6-Year-Old Chinese Children between 2004 and 2014
Authors: Xie Yixuan , Dai Yaohua , Li Tao
Published: May 2022

Childhood lead exposure is a commonly known risk factor affecting children’s health, and 10 governments have taken actions to reduce children’s lead exposure sources. Because lab testing for children’s blood lead levels (BLLs) was not popularized easily, socioeconomic and behavioural factors have been usually used as predictors of screening methods. Along with the overall decreasing trend of children’s BLLs, the lead-exposure-potential-predicting ability of such factors might be limited or changed over time. Our study aims to compare the predicting ability of multiple factors, including the living environment, economic disparity and personal behaviour differences between 2004 and 2014. With potential predicting factors identified, it could provide direction in identifying individual children facing high-risk lead exposure in the unit of clinics or communities of China. The study was first conducted in 12 cities in China in 2004 and then repeated in 2014 in the same 12 cities with the same method. In total, 27,972 children aged under 7 years were included in this study. With confounding factors adjusted, the child’s age, the family’s socioeconomic status and the child’s personal hygiene habit, especially biting toys, continued to be important predictors of higher blood lead levels among Chinese children. The sex of the child was no longer a predictor. Factors such as the father’s occupational contact with lead, residence near the main road and taking traditional Chinese medicine had the potential to be new predictors.
Investigation of Statistical Machine Learning Models for COVID-19 Epidemic Process Simulation: Random Forest, K-Nearest Neighbors, Gradient Boosting
Authors: Chumachenko Dmytro , Meniailov Ievgen , Bazilevych Kseniia , Chumachenko Tetyana , Yakovlev Sergey
Published: May 2022

COVID-19 has become the largest pandemic in recent history to sweep the world. This study is devoted to developing and investigating three models of the COVID-19 epidemic process based on statistical machine learning and the evaluation of the results of their forecasting. The models developed are based on Random Forest, K-Nearest Neighbors, and Gradient Boosting methods. The models were studied for the adequacy and accuracy of predictive incidence for 3, 7, 10, 14, 21, and 30 days. The study used data on new cases of COVID-19 in Germany, Japan, South Korea, and Ukraine. These countries are selected because they have different dynamics of the COVID-19 epidemic process, and their governments have applied various control measures to contain the pandemic. The simulation results showed sufficient accuracy for practical use in the K-Nearest Neighbors and Gradient Boosting models. Public health agencies can use the models and their predictions to address various pandemic containment challenges. Such challenges are investigated depending on the duration of the constructed forecast.
Clinical Translation of Microbiome Research in Alopecia Areata: A New Perspective?
Authors: Rinaldi Fabio , Trink Anna , Papale Angela , Giuliani Giammaria , Pinto Daniela
Published: May 2022

The continuous research advances in the microbiome field is changing clinicians’ points of view about the involvement of the microbiome in human health and disease, including autoimmune diseases such as alopecia areata (AA). Both gut and cutaneous dysbiosis have been considered to play roles in alopecia areata. A new approach is currently possible owing also to the use of omic techniques for studying the role of the microbiome in the disease by the deep understanding of microorganisms involved in the dysbiosis as well as of the pathways involved. These findings suggest the possibility to adopt a topical approach using either cosmetics or medical devices, to modulate or control, for example, the growth of overexpressed species using specific bacteriocins or postbiotics or with pH control. This will favour at the same time the growth of beneficial bacteria which, in turn, can impact positively both the structure of the scalp ecosystem on the host’s response to internal and external offenders. This approach, together with a “systemic” one, via oral supplementation, diet, or faecal transplantation, makes a reliable translation of microbiome research in clinical practice and should be taken into consideration every time alopecia areata is considered by a clinician.
Investigation on Step-Bunched Homoepitaxial Layers Grown on On-Axis 4H-SiC Substrates via Molten KOH Etching
Authors: Wang Jiulong , Zhao Siqi , Yan Guoguo , Shen Zhanwei , Zhao Wanshun , Wang Lei , Liu Xingfang
Published: May 2022

Wafer-scale on-axis 4H-SiC epitaxial layers with very low roughness were obtained in this study. By performing carbon-rich hydrogen etching and epitaxial growth of the epitaxial layer at different temperatures, local mirror regions (LMRs) with root mean square (RMS) roughness less than 0.2 nm were obtained on the epitaxial layer surface. The LMRs’ length is tens of millimeters, and the width is sub-millimeters. The step-flow growth induced by threading screw dislocations (TSDs) was observed on the epitaxial layer surface by atomic force microscopy (AFM), together with the double bi-atomic step-flow growth induced by the step bunch, which was the cause of LMRs. Furthermore, the growth mechanism was investigated by wet etching. The etching pits were found to be associated with 3C-SiC and their effect on the growth rate of epitaxial layers was further explored.
Seasonal Shifts in the Morphological Traits of Bloom-Forming Cyanobacteria in Lake Chaohu, China
Authors: Meng Yangyang , Zhang Min , Yang Zhen , Shi Xiaoli , Yu Yang , Shi Limei
Published: May 2022

Harmful cyanobacterial blooms in eutrophic water bodies pose a major threat to the environment and to human health. The morphological functional traits of cyanobacteria play important roles in maintaining their competitive advantages. To explore the regulatory mechanisms of the morphological functional traits of different bloom-forming cyanobacteria, we performed a one-year-long phytoplankton survey from November 2016 to October 2017 in Lake Chaohu, China. The colony size and cell diameter of the dominant cyanobacteria were measured, and their relationships were analyzed. The results showed that Dolichospermum flos-aquae and Microcystis aeruginosa were the dominant cyanobacteria in the lake. Microcystis was the dominant species during the summer; the growth of Dolichospermum growth surpassed that of Microcystis, and Dolichospermum became the dominant species in the late autumn, winter and spring. From winter to spring, the colony size of Dolichospermum decreased from 222.25 μm to 10.51 μm, and the individual cell diameter increased from 6.5 μm to 7.4 μm. From summer to autumn, Dolichospermum showed enlarged colony sizes and reduced cell diameters. The Microcystis colony size increased from 83.71 μm in the spring to 196.71 μm in the summer and autumn, while cells diameter remained essentially at 3–4 μm from March to October in Lake Chaohu. The relationship between colony size and cell diameter in Dolichospermum was significantly positive, while that of Microcystis was not significant. These results suggest that Dolichospermum may maintain biomass through a trade-off between cell diameter and colony size, and that a flexible morphological regulatory mechanism exists. This study seeks to improve our understanding of how bloom-forming cyanobacteria maintain their dominance by regulating their morphological traits.
Soil Arthropod Community in Spruce Forests (Picea abies) Affected by a Catastrophic Storm Event
Authors: Menta Cristina , Lozano Fondón Carlos , Remelli Sara
Published: May 2022

At the end of October 2018, the “Vaia” storm hit the eastern sector of the Italian Alps, causing major damage to forests. The resulting changes in habitat and resource availability are expected to shape the structure and abundance of soil communities. In this research, a soil arthropod community is studied one year after the catastrophic Vaia event in forests affected by the storm (W: Windthrow) to highlight the shift in the soil faunal community in a Mediterranean area increasingly impacted by climate change. Intact forests (IF) close to W were studied as a control condition and meadows (M) were considered to understand if W is moving toward a conversion to M or if the wooded character still prevails. Soil organic matter content was higher in IF than in W and M. The arthropod community was different between M and forests, both W and IF, while no differences were detected between W and IF considering the whole soil arthropod community. The Vaia catastrophic event does not appear to have radically changed the soil arthropod community and biodiversity after one year, despite upheaval to the vegetation cover, but the response is partially OTU (operative taxonomic unit)-specific. Hymenoptera adults and Coleoptera and Diptera larvae appear to be the most affected OTUs, showing lower abundance in W than IF. Conversely, Chilopoda seemed to benefit from the habitat changes, the result strongly related with the W condition. The two most present OTUs, Collembola and Acarina, were not affected by the Vaia storm. We may conclude that the soil system needs longer time to show a clear shift in the soil arthropod community.
An Improved Incipient Fault Diagnosis Method of Bearing Damage Based on Hierarchical Multi-Scale Reverse Dispersion Entropy
Authors: Xing Jiaqi , Xu Jinxue
Published: May 2022

The amplitudes of incipient fault signals are similar to health state signals, which increases the difficulty of incipient fault diagnosis. Multi-scale reverse dispersion entropy (MRDE) only considers difference information with low frequency range, which omits relatively obvious fault features with a higher frequency band. It decreases recognition accuracy. To defeat the shortcoming with MRDE and extract the obvious fault features of incipient faults simultaneously, an improved entropy named hierarchical multi-scale reverse dispersion entropy (HMRDE) is proposed to treat incipient fault data. Firstly, the signal is decomposed hierarchically by using the filter smoothing operator and average backward difference operator to obtain hierarchical nodes. The smoothing operator calculates the mean sample value and the average backward difference operator calculates the average deviation of sample values. The more layers, the higher the utilization rate of filter smoothing operator and average backward difference operator. Hierarchical nodes are obtained by these operators, and they can reflect the difference features in different frequency domains. Then, this difference feature is reflected with MRDE values of some hierarchical nodes more obviously. Finally, a variety of classifiers are selected to test the separability of incipient fault signals treated with HMRDE. Furthermore, the recognition accuracy of these classifiers illustrates that HMRDE can effectively deal with the problem that incipient fault signals cannot be easily recognized due to a similar amplitude dynamic.
Virtual Reality for Safe Testing and Development in Collaborative Robotics: Challenges and Perspectives
Authors: Badia Sergi Bermúdez i , Silva Paula Alexandra , Branco Diogo , Pinto Ana , Carvalho Carla , Menezes Paulo , Almeida Jorge , Pilacinski Artur
Published: May 2022

Collaborative robots (cobots) could help humans in tasks that are mundane, dangerous or where direct human contact carries risk. Yet, the collaboration between humans and robots is severely limited by the aspects of the safety and comfort of human operators. In this paper, we outline the use of extended reality (XR) as a way to test and develop collaboration with robots. We focus on virtual reality (VR) in simulating collaboration scenarios and the use of cobot digital twins. This is specifically useful in situations that are difficult or even impossible to safely test in real life, such as dangerous scenarios. We describe using XR simulations as a means to evaluate collaboration with robots without putting humans at harm. We show how an XR setting enables combining human behavioral data, subjective self-reports, and biosignals signifying human comfort, stress and cognitive load during collaboration. Several works demonstrate XR can be used to train human operators and provide them with augmented reality (AR) interfaces to enhance their performance with robots. We also provide a first attempt at what could become the basis for a human–robot collaboration testing framework, specifically for designing and testing factors affecting human–robot collaboration. The use of XR has the potential to change the way we design and test cobots, and train cobot operators, in a range of applications: from industry, through healthcare, to space operations.
Force-Feedback Haptic Device for Representation of Tugs in Virtual Reality
Authors: Lim Jongyoon , Choi Yongsoon
Published: May 2022

In this study, a haptic device is proposed for virtual reality (VR) applications. The proposed haptic device is linked to the bundle controller of a VR device. The device proposed in this study is a force-feedback device that focuses on the sensation felt by the hand rather than the feeling of the tool colliding with the object. The device provides sensations of centrifugal or inertial forces due to the weight of the tool when the user swings the tool and the sensation of a reaction force when the user stabs or pokes an object with the tool. The force generated by a haptic device of the linked type was measured and examined to determine whether a sensation of actually using a tool in the virtual environment was provided. In addition, we conducted two types of experiments to evaluate the proposed haptic device. First, VR content was created for the user experience, and a survey was conducted with 30 experimenters to compare the bundle controllers and a haptic device, called HapTug, in terms of realism, immersion, and enjoyment. The survey results demonstrated that the realism, immersion, and enjoyment increased compared to using the bundle controller alone. A total of 41 experimenters were allowed to play freely in a virtual environment for six sessions of 30 s each. They pressed the reset session button when they felt 30 s had passed, and the device they were wearing turned on and off randomly every session. During the experience, their playback time, number of interactions, and range were measured. As a result, when the device was activated, experimenters felt the time was shorter and showed widespread movement. Thus, it was proven that the proposed linked-type haptic device effectively delivered the feeling of wielding a tool, allowing the user to feel the virtual object coming in and going out of the hand when interacting with it. Moreover, three conclusions were drawn from the results of the experiments: previous VR experience times have little to do with satisfaction related to haptic device use, users have low expectations for haptic realism, and users interact more actively and exaggeratedly with a haptic device.
Modeling and Analysis of Voltage Ripple-Controlled SIDO Buck Converter in Pseudo-Continuous Conduction Mode with Limited Cross-Regulation and Fast Load Transient Performance
Authors: Jiao Haoyu , He Mingzhi , Feng Pan , Zhou Shuhan
Published: May 2022

Typical voltage mode-controlled DC–DC converters with single-inductor dual-output (SIDO) in pseudo-continuous conduction mode (PCCM) have slow load transient performance, and cross-regulation still exists between their two outputs. For the purposes of suppressing the cross-regulation and improving the load transient performance, a voltage ripple control technique for a PCCM SIDO buck converter is proposed in this study. A description of the PCCM SIDO buck converter and the voltage ripple control technique is provided in detail. In addition, the small-signal model was established, and the cross-regulation was further compared by Bode plots, as well as the load range and the power losses being analyzed. The cross-regulation and load transient performance of the voltage ripple-controlled PCCM SIDO buck converter was studied and compared with the conventional voltage mode-controlled. Using the proposed voltage ripple control for PCCM SIDO buck converters, we found no cross-regulation or rapid load transient behavior, which was validated by experimental results.
Bacterial Evolutionary Algorithm-Trained Interpolative Fuzzy System for Mobile Robot Navigation
Authors: Szili Ferenc Ádám , Botzheim János , Nagy Balázs
Published: May 2022

This paper describes the process of building a transport logic that enables a mobile robot to travel fast enough to reach a desired destination in time, but safe enough to prevent damage. This transport logic is based on fuzzy logic inference using fuzzy rule interpolation, which allows for accurate inferences even when using a smaller rule base. The construction of the fuzzy rule base can be conducted experimentally, but there are also solutions for automatic construction. One of them is the bacterial evolutionary algorithm, which is used in this application. This algorithm is based on the theory of bacterial evolution and is very well-suited to solving optimization problems. Successful transport is also facilitated by proper path planning, and for this purpose, the so-called neuro-activity-based path planning has been used. This path-planning algorithm is combined with interpolative fuzzy logic-based speed control of the mobile robot. By applying the described methods, an intelligent transport logic can be constructed. These methods are tested in a simulated environment and several results are investigated.
Techno-Economic Evaluation of 5G-NSA-NPN for Networked Control Systems
Authors: Kiesel Raphael , Schmitt Sarah , König Niels , Brochhaus Maximilian , Vollmer Thomas , Stichling Kirstin , Mann Alexander , Schmitt Robert H.
Published: May 2022

Wireless closed-loop control systems, so-called networked control systems (NCS) promise technical and economic benefits for production applications. To realize prospective benefits, the right communication technology is key. The fifth generation of mobile communication is predicted to have a significant impact on the deployment of NCS in the industrial connectivity landscape. However, there are different options for 5G deployment influencing both technical performance and economic aspects of the network. This in turn is expected to have a techno-economic influence on the production itself. Thus, a trade-off between the necessary technical performance of the 5G network and the benefits for the production must be executed. This paper, therefore, aims to analyze the techno-economic benefits of 5G deployment for closed-loop control systems in production. To reach this aim, first, the fundamentals of techno-economic analysis are introduced. Second, the results of an experimental performance analysis of a 5G-NSA-NPN at Fraunhofer IPT in Aachen are shown. Third, based on the results from the experimental study, a model-based techno-economic ex-ante evaluation of 5G-NSA-NPN for closed-loop applications is performed, and an exemplar is shown for a BLISK milling use case. Finally, the results are summarized and an outlook for further research is given. The analysis shows a difference in net present value for 5G deployment of EUR 2.6 M after 10 years and a difference of OPEX per product of around EUR −1000 per BLISK. Furthermore, analysis shows an increase in productivity (0.73%), quality (30.75%), and sustainability (2.87%). This indicates a noticeable improvement of a 5G-controlled NCS.