bluuebunny/crossref_metadata_2025 huggingface dataset:
An Improved Method for Quick Quantification of Unsaturated Transferrin
Authors: Guo Ruirui , Gao Juanjuan , Hui Lingyun , Li Yanqing , Liu Junhui , Fu Yao , Shi Lei , Wang Yawen , Liu Bing
Published: September 2022

Blood iron levels play a vital role in oxygen metabolism and energy generation whilst transporter protein, transferrin, binds and delivers iron to the transferrin receptor of endosomal compartments of cells. Consequently, the iron-binding capacity of transferrin is an important indicator for many diseases, and its measurements are used in the diagnosis and treatment of anaemias. Various assays, including Total Iron Binding Capacity (TIBC), Unsaturated Iron-Binding Capacity (UIBC) and Transferrin Saturation (TS), were developed to assess the iron-binding capacity of transferrin. Clinically, UIBC is measured in serum by a multi-step liquid ferrozine method and subjected to interference from conditions such as haemolysis and lipemia. Here, we report a quick method that directly measures the concentration of apotransferrin in EDTA-treated plasma, theoretically equivalent to UIBC. Importantly, this supramolecular assembly-based method is more time-efficient, cost-effective and insensitive to serum cation fluctuations. With additional colorimetric property, this method also provides a visual indicator for abnormal health conditions with extreme transferrin statuses such as those found in cancers. Its minimal requirement for equipment would be particularly useful for diagnosis in remote and under-developed regions.
Performance of Bolt-Welded CFST Short Columns with Different Initial Imperfections: Experimental and Numerical Studies
Authors: Yu Peng , Ren Zhaoyong , Yun Weijing , Zhao Ye , Xu Jinglei
Published: September 2022

Gap between the steel tube and core concrete of Concrete-Filled Steel Tube (CFST) members is regarded as an initial imperfection, which may exist in the CFST arch bridge due to construction process problems. In this paper, based on the design scheme of the bolt-welded joints in arch rib columns of an extra-long span CFST arch bridge, the effects of two types of initial imperfections, interfacial gap and spherical-cap gap, on the mechanical properties of Bolt-Welded CFST (BWCFST) short column under axial compression were investigated. Axial compression tests were conducted on three column specimens with spherical-cap gap, three specimens with interfacial gap, and one normal specimen without imperfection. A Finite Element Analysis (FEA) model was developed to further investigate the behavior of the BWCFST short columns, and was validated by the experiment.
Numerical Optimization for the Design of Geopolymer Concrete Walls with Phase Change Materials in the Mediterranean Climate
Authors: Ascione Fabrizio , De Masi Rosa Francesca , Del Regno Nicoletta , De Rossi Filippo , Gigante Antonio , Ruggiero Silvia
Published: September 2022

The adoption of phase change materials (PCMs) is a promising solution for the improvement of building energy performances and indoor comfort, and the integration of geopolymer concrete (GPC) allows recycling materials and reducing the demand for raw materials in concrete production. Both materials contribute to reducing the carbon dioxide emission in the building lifecycle. In this frame, this paper proposes a complete numerical approach for selecting the optimal wall package made of GPC and PCMs in a Mediterranean climate. The first step of the method consists of a parametric analysis for evaluating the incidence on energy performance and thermal comfort of the main designing variables: insulation thickness, air cavity type and its thickness, and PCMs type. Then, assuming the discomfort hours as a limiting constraint, a multi-objective optimization is applied to a subset of solutions for determining the Pareto front solutions. The advantage of the proposed methodology is the combined evaluations of multiple variables with a simplicity in execution; for this reason, it is useful for other researchers aimed at studying innovative solutions. According to obtained results, the better exposure for the proposed wall package is the north or northeast one. The minimization of the cooling energy demand requires the adoption of two PCMs, on internal and external sides, with melting temperature of 26 °C. The optimization of yearly performance requires the adoption of the maximum insulation level on both sides and a not-ventilated air gap between the modules. The cooling and heating energy need can be reduced, respectively, by around −29% and −57%, compared to a reference configuration with vacuum insulation panels and thermal transmittance of 0.4 W/m2 K.
Current State of Using Prefabricated Construction in Australia
Authors: Zhang Zhiming , Tan Yongtao , Shi Long , Hou Lei , Zhang Guomin
Published: September 2022

The Australian prefabricated construction market has been developing rapidly in recent years. New prefabrication-related technologies, materials, systems and services are also emerging in the current Australian market. Although some studies have been undertaken to explore the benefits and challenges of implementing prefabrication in Australia over the past 15 years, they do not reflect the recent changes in the industry. Therefore, this study aims to fill this gap and identify the major changes in the current Australian prefabricated construction industry from industrial perceptions. Through literature reviews and industry interviews, factors reflecting major changes in the current Australian prefabricated construction, including prefabrication industry development, emerging benefits and challenges, were identified and discussed in this study. The challenges identified from interviews were classified into eight aspects related to feasibility, design, manufacturing, transportation, on-site construction, standardisation, skills and knowledge, finance and market. Furthermore, 21 recommendations and related key responsible parties were identified to tackle these challenges. The findings will provide useful references for various stakeholders to have a better understanding of the current prefabrication industry development in the Australian context and re-think how to adapt to future changes for the uptake of prefabricated construction in Australia.
Photocatalytic Degradation of Magenta Effluent Using Magnetite Doped TiO2 in Solar Parabolic Trough Concentrator
Authors: Pucar Milidrag Gordana , Nikić Jasmina , Gvoić Vesna , Kulić Mandić Aleksandra , Agbaba Jasmina , Bečelić-Tomin Milena , Kerkez Djurdja
Published: September 2022

Due to population growth and industrial development consumption of non-renewable energy sources, and consequently pollution, has increased. In order to reduce energy utilisation and preserve the environment, developed and developing countries are increasingly trying to find solutions based on renewable energy sources. Cost-effective wastewater treatment methods using solar energy would significantly ensure effective water source utilisation, thereby contributing towards sustainable development goals. In this paper, special emphasis is given to the use of solar energy as the driving force of the process, as well as the use of highly active magnetic TiO2-based catalysts. Therefore, in this study, we investigated the possibility of photocatalytic degradation of aqueous magenta graphic dye using titanium dioxide as a catalyst and DSD model in order to achieve the best process optimisation. TiO2 was successfully coated with magnetic nanoparticles by one step process and characterized using different techniques (BET, SEM/EDS, FTIR, XRD). Based on DSD statistical method optimal reaction conditions were pH = 6.5; dye concentration 100 mg/L; TiO2–Fe3O4 0.6 g/L, at which the highest degree of magenta dye decolourisation was achieved (85%). Application of solar energy coupled with magnetic TiO2 catalyst which could be recovered and reused makes this approach a promising alternative in green wastewater treatment.
Enhancing Photocatalysis of Ag Nanoparticles Decorated BaTiO3 Nanofibers through Plasmon-Induced Resonance Energy Transfer Turned by Piezoelectric Field
Authors: Chen Peng , Li Xiu , Ren Zeqian , Wu Jizhou , Li Yuqing , Liu Wenliang , Li Peng , Fu Yongming , Ma Jie
Published: September 2022

Revealing the charge transfer path is very important for studying the photocatalytic mechanism and improving photocatalytic performance. In this work, the charge transfer path turned by the piezoelectricity in Ag-BaTiO3 nanofibers is discussed through degrading methyl orange. The piezo-photocatalytic degradation rate of Ag-BaTiO3 is much higher than the photocatalysis of Ag-BaTiO3 and piezo-photocatalysis of BaTiO3, implying the coupling effect between Ag nanoparticle-induced localized surface plasmon resonance (LSPR), photoexcited electron-hole pairs, and deformation-induced piezoelectric field. With the distribution density of Ag nanoparticles doubling, the LSPR field increases by one order of magnitude. Combined with charge separation driven by the piezoelectric field, more electrons in BaTiO3 nanofibers are excited by plasmon-induced resonance energy transfer to improve the photocatalytic property.
Understanding 2D-IR Spectra of Hydrogenases: A Descriptive and Predictive Computational Study
Authors: Rippers Yvonne , Procacci Barbara , Hunt Neil T. , Horch Marius
Published: September 2022

[NiFe] hydrogenases are metalloenzymes that catalyze the reversible cleavage of dihydrogen (H2), a clean future fuel. Understanding the mechanism of these biocatalysts requires spectroscopic techniques that yield insights into the structure and dynamics of the [NiFe] active site. Due to the presence of CO and CN− ligands at this cofactor, infrared (IR) spectroscopy represents an ideal technique for studying these aspects, but molecular information from linear IR absorption experiments is limited. More detailed insights can be obtained from ultrafast nonlinear IR techniques like IRpump-IRprobe and two-dimensional (2D-)IR spectroscopy. However, fully exploiting these advanced techniques requires an in-depth understanding of experimental observables and the encoded molecular information. To address this challenge, we present a descriptive and predictive computational approach for the simulation and analysis of static 2D-IR spectra of [NiFe] hydrogenases and similar organometallic systems. Accurate reproduction of experimental spectra from a first-coordination-sphere model suggests a decisive role of the [NiFe] core in shaping the enzymatic potential energy surface. We also reveal spectrally encoded molecular information that is not accessible by experiments, thereby helping to understand the catalytic role of the diatomic ligands, structural differences between [NiFe] intermediates, and possible energy transfer mechanisms. Our studies demonstrate the feasibility and benefits of computational spectroscopy in the 2D-IR investigation of hydrogenases, thereby further strengthening the potential of this nonlinear IR technique as a powerful research tool for the investigation of complex bioinorganic molecules.
Influence of Tamoxifen on Different Biological Pathways in Tumorigenesis and Transformation in Adipose-Derived Stem Cells, Mammary Cells and Mammary Carcinoma Cell Lines—An In Vitro Study
Authors: Schlottmann Frederik , Bucan Vesna , Strauß Sarah , Koop Felix , Vogt Peter M. , Mett Tobias R.
Published: September 2022

Breast carcinoma is one of the most common malignant tumors in women. In cases of hormone-sensitive cells, tamoxifen as an anti-estrogenic substance is a first line medication in the adjuvant setting. The spectrum of autologous breast reconstructions ranges from fat infiltrations to complex microsurgical procedures. The influence of adipose-derived stem cells (ASC) on the tumor bed and a possibly increased recurrence rate as a result are critically discussed. In addition, there is currently no conclusive recommendation regarding tamoxifen-treated patients and autologous fat infiltrations. The aim of the present study was to investigate the effect of tamoxifen on the gene expression of a variety of genes involved in tumorigenesis, cell growth and transformation. Mammary epithelial cell line and mammary carcinoma cell lines were treated with tamoxifen in vitro as well as co-cultured with ASC. Gene expression was quantified by PCR arrays and showed increased expression in the mammary carcinoma cell lines with increasing time of treatment and concentration of tamoxifen. The data presented can be considered as an addition to the controversial discussion on the relationship between ASC and breast carcinoma cells. Further studies are needed to quantify the in vivo interaction of ASC and mammary carcinoma cells and to conclusively assess the impact of tamoxifen in reconstructive cases with fat grafting.
Apigetrin Promotes TNFα-Induced Apoptosis, Necroptosis, G2/M Phase Cell Cycle Arrest, and ROS Generation through Inhibition of NF-κB Pathway in Hep3B Liver Cancer Cells
Authors: Bhosale Pritam Bhagwan , Abusaliya Abuyaseer , Kim Hun Hwan , Ha Sang Eun , Park Min Yeong , Jeong Se Hyo , Vetrivel Preethi , Heo Jeong Doo , Kim Jin-A , Won Chung kil , Kim Hyun-Wook , Kim Gon Sup
Published: September 2022

Apigetrin (7-(β-D-glucopyranosyloxy)-4′,5-dihydroxyflavone), a glycoside bioactive dietary flavonoid derived from Taraxacum officinale and Teucrium gnaphalodes, is known to possess anticancer, antioxidant, and anti-inflammatory effects on numerous cancers. In the present study, we examined the effect of apigetrin in Hep3B hepatocellular cancer cell line (HCC). Apigetrin inhibited cell growth and proliferation of Hep3B cells, as confirmed by MTT and colony formation assay. We used apigetrin at concentrations of 0, 50, and 100 µM for later experiments. Of these concentrations, 100 µM of apigetrin showed a significant effect on cell inhibition. In apigetrin-treated Hep3B cells, cell cycle arrest occurred at the G2/M phase. Apoptosis and necroptosis of Hep3B cells treated with apigetrin were confirmed by Annexin V/propidium iodide (PI) staining and flow cytometry results. Morphological observation through 4′,6-diamidino-2-phenylindole (DAPI) staining showed intense blue fluorescence representing chromatin condensation. Hematoxylin staining showed necroptotic features such as formation of vacuoles and swelling of organelles. Apigetrin increased reactive oxygen species (ROS) levels in cells, based on fluorescence imaging. Furthermore, the underlying mechanism involved in the apoptosis and necroptosis was elucidated through western blotting. Apigetrin up-regulated TNFα, but down-regulated phosphorylation of p-p65, and IκB. Apigetrin inhibited the expression of Bcl-xl but increased Bax levels. Up-regulation of cleaved PARP and cleaved caspase 3 confirmed the induction of apoptosis in apigetrin-treated Hep3B cells. Additionally, necroptosis markers RIP3, p-RIP3, and p-MLKL were significantly elevated by apigetrin dose-dependently, suggesting necroptotic cell death. Taken together, our findings strongly imply that apigetrin can induce apoptosis and necroptosis of Hep3B hepatocellular cancer cells. Thus, apigetrin as a natural compound might have potential for treating liver cancer.
Postnatal and Adult Neurogenesis in Mammals, Including Marsupials
Authors: Bartkowska Katarzyna , Tepper Beata , Turlejski Krzysztof , Djavadian Ruzanna
Published: September 2022

In mammals, neurogenesis occurs during both embryonic and postnatal development. In eutherians, most brain structures develop embryonically; conversely, in marsupials, a number of brain structures develop after birth. The exception is the generation of granule cells in the dentate gyrus, olfactory bulb, and cerebellum of eutherian species. The formation of these structures starts during embryogenesis and continues postnatally. In both eutherians and marsupials, neurogenesis continues in the subventricular zone of the lateral ventricle (SVZ) and the dentate gyrus of the hippocampal formation throughout life. The majority of proliferated cells from the SVZ migrate to the olfactory bulb, whereas, in the dentate gyrus, cells reside within this structure after division and differentiation into neurons. A key aim of this review is to evaluate advances in understanding developmental neurogenesis that occurs postnatally in both marsupials and eutherians, with a particular emphasis on the generation of granule cells during the formation of the olfactory bulb, dentate gyrus, and cerebellum. We debate the significance of immature neurons in the piriform cortex of young mammals. We also synthesize the knowledge of adult neurogenesis in the olfactory bulb and the dentate gyrus of marsupials by considering whether adult-born neurons are essential for the functioning of a given area.
A Critical Review of the Use of Graphene-Based Gas Sensors
Authors: Chakraborthy Aniket , Nuthalapati Suresh , Nag Anindya , Afsarimanesh Nasrin , Alahi Md. Eshrat E , Altinsoy Mehmet Ercan
Published: September 2022

The employment of graphene for multifunctional uses has been a cornerstone in sensing technology. Due to its excellent electrochemical properties, graphene has been used in its pure and composite forms to detect target molecules over a wide range of surfaces. The adsorption process on the graphene-based sensors has been studied in terms of the change in resistance and capacitance values for various industrial and environmental applications. This paper highlights the performance of graphene-based sensors for detecting different kinds of domestic and industrial gases. These graphene-based gas sensors have achieved enhanced output in terms of sensitivity and working range due to specific experimental parameters, such as elevated temperature, presence of particular gas-specific layers and integration with specific nanomaterials that assist with the adsorption of gases. The presented research work has been classified based on the physical nature of graphene used in conjugation with other processed materials. The detection of five different types of gases, including carbon dioxide (CO2), ammonia (NH3), hydrogen sulphide (H2S), nitrogen dioxide (NO2) and ethanol (C2H5OH) has been shown in the paper. The challenges of the current graphene-based gas sensors and their possible remedies have also been showcased in the paper.
Polymer-Based High Diffraction Efficiency and High Resolution Volume Holographic Transmission Gratings
Authors: Castagna Riccardo , Di Donato Andrea , Francescangeli Oriano , Lucchetta Daniele Eugenio
Published: September 2022

We report on the optical characterization of very high-efficiency and high-resolution holographic volume phase transmission gratings. The gratings are recorded in a new photo-polymerizable mixture made by epoxy-resin and multi-acrylate. The epoxy-resin used is known to make tenacious acrylate-based films. The holographic mixture contains two photo-initiators, the synergic effect of which enables a reliable photo-polymerization process in the visible region of the electromagnetic spectrum. The recorded holograms are mechanically stable, show long-term temporal stability and very high values of diffraction efficiency, coupled with good angular selectivity due to a relatively narrow band of wavelengths. We measured the intensity of the transmitted beam and calculated the intensity of the diffracted beam at different wavelengths, deriving the refractive index modulation and the grating pitch by fitting the experimental data with a slightly modified theoretical approach. These kind of mixtures can be used in several fields of application, such as chemical or bio-sensors, high resolution optical sensors, high-density optical data storage, encryption and security.
Sensory Profile of Children and Adolescents with Autism Spectrum Disorder and Tip-Toe Behavior: Results of an Observational Pilot Study
Authors: Valagussa Giulio , Purpura Giulia , Nale Alessandra , Pirovano Rita , Mazzucchelli Miryam , Grossi Enzo , Perin Cecilia
Published: September 2022

Atypical sensory processing is frequently reported in persons with autism spectrum disorders (ASD), and it is one of the described diagnostic criteria for ASD. There is also mounting literature supporting the presence of motor impairments in individuals with ASD. Among these motor signs, tip-toe behavior (TTB) is a possible clinical finding, but its etiology is not clearly understood. It is suggested that TTB in ASD could be a sign of a sensory modulation impairment, but evidence is lacking and controversial. The main aim of this pilot study is to explore sensory features in a sample (4 females; 28 males) of children and adolescents with ASD (age range: 7–18). All participants also presented Intellectual Disability. Participants were divided in two groups, matched for age and gender, on the basis of the presence or absence of TTB (16 ASD TTB group vs. 16 ASD NO-TTB group) and then evaluated by using the Short Sensory Profile. We found that both ASD groups tend to significantly present sensory-related behavioral symptoms, but ASD TTB individuals more frequently showed the specific pattern of “under responsive/seeks sensation” than ASD NO-TTB individuals. These preliminary findings support that sensory-motor features might be taken into consideration when rehabilitation for TTB in children and adolescents with ASD is necessary.
Novel Ensemble Model Recommendation Approach for the Detection of Dyslexia
Authors: AlGhamdi Ahmed Saeed
Published: September 2022

There are a large number of neurological disorders being explored regarding possible management and treatment, with dyslexia being one of the disorders that affect children at the onset of their learning process. Dyslexia is a developmental neurological disorder that prevents children from learning. The disorder has a prevalence of around 10% across the globe, as reported by most of the literature on dyslexia. The early detection and management of dyslexia is one of the primary pursuits among different research. One such domain that leads this pursuit of the early detection and management of dyslexia is artificial intelligence. With so much effort being expended to explore the applicability of artificial intelligence to address the problem of dyslexia detection, in this work, an ensemble model for the early detection of dyslexia is proposed and recommend. The work experimentally considers a pool of ensembles with rigorous validation on a large sized dataset. The final ensemble model recommendation for detection is expressed after evaluating all of the ensemble frameworks based on a number of evaluation parameters. Our experiments reveal that the subspace discriminant ensemble showed superiority for the detection of dyslexia with an accuracy of 90% on five-fold cross validation with the least training time. An accuracy of 90.90% was achieved using boosted trees with a holdout validation of 30%, while with no validation the subspace K-Nearest Neighbor (KNN) outperformed the other ensembles with an accuracy of 99.9%.
Severe Hypercapnia Requiring 48-h Whole-Body Hypothermia in an Infant with Acute Bronchiolitis
Authors: Librandi Michela , Scapaticci Serena , Chiavaroli Valentina , Petrucci Altea , Cicioni Paola , Cognigni Rita , Chiarelli Francesco , Di Valerio Susanna
Published: September 2022

Bronchiolitis is a clinical syndrome involving the lower respiratory tract of infants and young children. The majority of patients recover using adequate hydration and oxygen (O2) therapy, while a small number of patients require ventilatory assistance. Beyond these therapeutical approaches, there are no available strategies for patients that do not improve. Hypothermia is a measure used to prevent neonatal hypoxic–ischemic encephalopathy by preventing carbon dioxide (CO2) production and subsequent tissue damage. Other medical applications of hypothermia have been proposed, such as in acute respiratory failure and necrotizing colitis. Case report: We report the case of a 50-day-old girl hospitalized with severe bronchiolitis caused by respiratory syncytial virus. On admission, the girl presented severe hypercapnic respiratory failure, requiring intubation and ventilatory support with conventional and non-conventional systems. However, the patient’s general conditions worsened with elevated O2 demand, thus whole-body hypothermia was attempted and performed for 48 h, with a gradual improvement in the respiratory function. No adverse effects were detected. Conclusions: Whole-body hypothermia could have a critical role as a rescue treatment in infants affected by severe hypercapnic respiratory failure, at the expense of few and rare side effects (bradycardia, coagulopathy, hyperglycemia). Notably, beyond reducing CO2 production, whole-body hypothermia might have an impact in restoring lung function in newborns using bronchiolitis refractory to maximal medical therapy and invasive ventilation.
Needs, Barriers and Facilitators of Adolescents Participating in a Lifestyle Promotion Program in Oncology: Stakeholders, Adolescents and Parents’ Perspective
Authors: Kerba Johanne , Demers Catherine , Bélanger Véronique , Napartuk Mélanie , Bouchard Isabelle , Meloche Caroline , Morel Sophia , Prud’homme Nicolas , Gélinas Isabelle , Higgins Johanne , Curnier Daniel , Sultan Serge , Laverdière Caroline , Sinnett Daniel , Marcil Valérie
Published: September 2022

Treatments for adolescent cancer can cause debilitating side effects in the short- and long-term such as nausea and malnutrition but also cardiometabolic disturbances. Although the risk for cardiometabolic complications is greater for adolescents with cancer than younger ones, adolescents typically respond poorly to family-oriented health promotion programs. This study aims to assess the needs, barriers and facilitators to healthy lifestyle promotion interventions for adolescents with cancer and how to best adapt these interventions for them. Interviews were held with adolescents treated for cancer (n = 9) and parents (n = 6), focus groups were conducted with stakeholders working in oncology (n = 12) and self-report questionnaires were sent to stakeholders involved in a health promotion intervention (n = 6). At the time of interview, mean age of adolescent participants (40% female) was 17.0 ± 1.9 years (mean age at diagnosis: 14.6 ± 1.6 years). Verbatim and responses to questionnaires were coded and analyzed using qualitative methods. Stakeholder stated that adolescents with cancer need to access activities adapted to their age, to communicate with peers going through a similar experience, and to preserve their schooling and friendships. Barriers to intervention reported by adolescents, parents and stakeholders include lack of motivation, schedule conflicts, fatigue and treatment side effects. Some of the barriers mentioned by adolescents and parents include pain, post-surgery problems, school, physical deconditioning, and lack of time. Facilitators mentioned by adolescents and parents comprise trust in stakeholders’ expertise, personalized approaches, scheduling flexibility. Stakeholders recommended to build trust in the relationship, favoring non-moralizing teachings, adapt interventions to adolescents’ limited attention span and avoiding the use of long-term health benefits as a motivator.
The Level and Factors Differentiating the Physical Fitness of Adolescents Passively and Actively Resting in South-Eastern Poland—A Pilot Study
Authors: Puchalska-Sarna Anna , Baran Rafał , Kustra Magdalena , Pop Teresa , Herbert Jarosław , Baran Joanna
Published: September 2022

Physical activity (PA) is defined as any bodily movement produced by skeletal muscles that requires energy expenditure. Due to civilization’s development, we can observe a global decline in physical activity which negatively affects the state of physical and mental health. The physical activity of children and adolescents is a counterpart to their physical fitness. There is also more frequent spending of free time in a passive way rather than actively. The aim of the study was to determine whether there are differences in the physical fitness of young people who rest passively in relation to those who rest actively. In addition, it was checked whether factors, such as age, weight, body height and BMI differentiate the level of fitness in adolescents. Study group: 25 boys and 25 girls declaring active leisure activities. Control group: 25 boys and 25 girls declaring passive leisure activities. Age of the respondents ranged from 11 to 15 years (Me = 13; SD = 1.23). The research used: the author’s questionnaire and the Index of Physical Fitness of K. Zuchora. The results were statistically developed. The youth who spend their free time actively were characterised by a higher level of physical fitness than their peers who choose passive recreation. The students with a higher BMI obtained worse results than the children with a lower body mass index. In both groups, slightly better results were obtained by girls. A significant relationship between age and results has been observed in the control group—the results increased with increasing age. The level of physical fitness is higher in active forms of recreation than in passive rest. Physical fitness tends to increase with age but decreases with increasing BMI. Girls are characterised by a higher level of physical fitness than boys.
Reappraisal of VEGF in the Pathogenesis of Kawasaki Disease
Authors: Chen Chun-Yu , Huang Shih-Hui , Chien Kuang-Jen , Lai Tsung-Jen , Chang Wei-Hsiang , Hsieh Kai-Sheng , Weng Ken-Pen
Published: September 2022

Vascular endothelial growth factor (VEGF) is an important factor in mediating the inflammation of Kawasaki disease (KD). The literature regarding the relationship between VEGF and KD is sparse. The purpose of this study was to investigate the correlation of VEGF and KD. In a prospective study of 42 Taiwanese KD patients (18.9 ± 12.2 months, M/F 22/20) treated with intravenous immunoglobulin (IVIG), a series of VEGF levels was measured from the acute to convalescent phases. KD patients were classified into two subgroups with (n =20) and without (n = 22) acute coronary artery lesions (CALs). Control samples were obtained from 30 febrile controls (19.1 ± 13.7 months, M/F 13/17). In KD patients, VEGF levels in the pre-IVIG acute phase were significantly higher than those in the subacute and convalescent phases (both p < 0.001). In KD patients with CALs, VEGF levels significantly increased immediately in the post-IVIG phase (p = 0.039), and then significantly decreased in the subacute phase (p = 0.002). KD patients with acute CALs had higher median VEGF levels than those without acute CALs from acute to convalescent phases. In the subacute phase, KD patients with acute CALs had significantly higher VEGF levels (p = 0.022) than those without acute CALs. Our data show that VEGF did not decrease after IVIG treatment, and increased significantly after IVIG treatment in KD patients with acute CALs in acute phase. VEGF might be related to the complications of CALs in KD patients.
COPS5 Conferred the Platinum Resistance in Epithelial Ovarian Cancer
Authors: Zhang Hongqin , Yan Tianqing , Zhong Ailing , Guo Lin , Lu Renquan
Published: September 2022

Development of platinum resistance is one of the major causes of epithelial ovarian cancer (EOC) treatment failure. COP9 signalosome subunit 5 (COPS5) was found to take part in the progression of EOC in our previous study. Herein, we aim to uncover the potential utility of COPS5 in EOC chemoresistance. COPS5 levels were analyzed to define clinic pathologic correlates using a matched tissue microarray and online datasets. The effect of COPS5 inhibition by the lentivirus-mediated short hairpin RNA on cell viability, proliferation and migration was accessed in vitro and in vivo. Results showed that COPS5 was upregulated in patients after platinum resistance. Kaplan–Meier survival curves revealed that COPS5 overexpression was correlated with shorter PFS and OS. COPS5 downregulation inhibited the cell proliferation, migration, and reduced the sensitivity of EOC to platinum. Overall, our data indicated that COPS5 inhibition might represent a new therapeutic strategy for overcoming platinum resistance in patients with EOC.
Efficient Management of Sewage Sludge from Urban Wastewaters with the Addition of Inorganic Waste: Focus on Rheological Properties
Authors: Santos Andreia F. , Ferreira Abel G. M. , Quina Margarida J.
Published: September 2022

Sewage sludge (SS) from urban wastewater treatment is still an environmental, economic, and social problem. Current SS management is not consensual, and more alternatives are required to recover some valuable compounds, such as nutrients and organic matter. This study investigates the use of green liquor dregs from the pulp and paper industry—GLDs—as an adjuvant of drying, to develop a product for agronomic applications, focusing on the rheological behavior. The rheological properties were assessed for anaerobically digested sludge (ADS). The limit viscosity of raw ADS was about 0.005 Pa·s in the case of 5% TSs (total solids) increasing to 0.51 Pa·s for 20% TSs. From the oscillatory tests, the ideal viscous flow below 10% TSs was observed, whereas a viscoelastic–solid behavior was detected for a higher concentration of TSs. The addition of GLDs to the ADS reduced the consistency index, reducing the shear resistance of the material. Rheological assays showed that GLDs may facilitate sludge handling (e.g., extrusion) from the dewatering unit to the dryer. Overall, the addition of GLDs to ADS showed to be a viable option for drying and subsequent soil application. Reusing both residues promote the transition from a linear to a circular economy in the wastewater treatment sector.
New Air Temperature- and Wind Speed-Based Clothing Thermal Resistance Scheme—Estimations for the Carpathian Region
Authors: Ács Ferenc , Kristóf Erzsébet , Szabó Amanda Imola , Breuer Hajnalka , Szalkai Zsófia , Zsákai Annamária
Published: September 2022

A new clothing thermal resistance scheme is presented and verified for the Carpathian region and for the time period 1971–2000. The scheme is as simple as possible by connecting operative temperature to air temperature, which allows for it to only use air temperature and wind speed data as meteorological inputs. Another strength of the scheme is that a walking person’s metabolic heat flux density is also simply simulated without having to regard any thermoregulation processes. Human thermal load in the above region is characterised by a representative adult Hungarian male and female with a body mass index of 23–27 kgm−2. Our most important findings are as follows: (1) human thermal load in the Carpathian region is relief dependent; (2) the scheme cannot be applied in the lowland areas of the region in the month of July since the energy balance is not met; (3) in the same areas but during the course of the year, clothing thermal resistance values are between 0.4 and 1 clo; (4) clothing thermal resistance can reach 1–1.2 clo in the mountains in the month of July, but during the course of the year this value is 1.8 clo; and (5) the highest clothing thermal resistance values can be found in January reaching about 2.5 clo. The scheme may be easily applied to any another region by determining new, region-specific, operative temperature–air temperature relationships.
Dreaming and Sleep-Related Metacognitions in Patients with Sleep Disorders
Authors: Schredl Michael , Schilling Claudia
Published: September 2022

Sleep-related metacognitions play a role in the etiology of insomnia and are distressing while falling asleep. Although similar concepts, such as thought suppression, have been studied in the context of dreaming, the relationship between sleep-related metacognitions and more negatively toned dreaming due to stressful pre-sleep experiences has yet to be studied. Overall, 919 patients with various sleep disorders completed the Metacognitions Questionnaire-Insomnia (MCQ-I20), Arousal Disposition Scale (APS), and Pre-Sleep Arousal Scale (PSAS) and kept a sleep diary over seven days eliciting dream recall, nightmare frequency, and the emotional tone of their dreams. The regression analysis showed that the MCQ-I20 (small effect size) and the APS (medium effect size) were associated with nightmare frequency and negatively toned dream emotions. These findings suggest that dysfunctional sleep-related metacognitions that are active prior to sleep are also associated with more negatively toned dreaming and more nightmares—even after controlling for trait arousability. It would be very interesting to study where therapeutic strategies, such as metacognitive therapy explicitly targeting sleep-related metacognition, could also be beneficial with regard to dreams (more positive dreams and fewer nightmares).
Hardware Limitations of Lightweight Cryptographic Designs for IoT in Healthcare
Authors: Tsantikidou Kyriaki , Sklavos Nicolas
Published: September 2022

Security is an important aspect of healthcare applications that employ Internet of Things (IoT) technology. More specifically, providing privacy and ensuring the confidentiality, integrity and authenticity of IoT-based designs are crucial in the health domain because the collected data are sensitive, and the continuous availability of the system is critical for the user’s wellbeing. However, the IoT consists of resource-constrained devices that increase the difficulty of implementing high-level-security schemes. Therefore, in the current paper, renowned lightweight cryptographic primitives and their most recent architecture, to the best of the authors’ knowledge, are investigated. Their security, architecture characteristics and overall hardware limitations are analyzed and collected in tables. Finally, all the algorithms are compared based on their effectiveness in securing healthcare applications, the utilized device and the overall implementation efficiency.
Revealing the Complete Chloroplast Genome of an Andean Horticultural Crop, Sweet Cucumber (Solanum muricatum), and Its Comparison with Other Solanaceae Species
Authors: Saldaña Carla L. , Chávez-Galarza Julio C. , De la Cruz Germán , Jhoncon Jorge H. , Guerrero-Abad Juan C. , Vásquez Héctor V. , Maicelo Jorge L. , Arbizu Carlos I.
Published: September 2022

Sweet cucumber (Solanum muricatum) sect. Basarthrum is a neglected horticultural crop native to the Andean region. It is naturally distributed very close to other two Solanum crops of high importance, potatoes, and tomatoes. To date, molecular tools for this crop remain undetermined. In this study, the complete sweet cucumber chloroplast (cp) genome was obtained and compared with seven Solanaceae species. The cp genome of S. muricatum was 155,681 bp in length and included a large single copy (LSC) region of 86,182 bp and a small single-copy (SSC) region of 18,360 bp, separated by a pair of inverted repeats (IR) regions of 25,568 bp. The cp genome possessed 87 protein-coding genes (CDS), 37 transfer RNA (tRNA) genes, eight ribosomal RNA (rRNA) genes, and one pseudogene. Furthermore, 48 perfect microsatellites were identified. These repeats were mainly located in the noncoding regions. Whole cp genome comparative analysis revealed that the SSC and LSC regions showed more divergence than IR regions. Similar to previous studies, our phylogenetic analysis showed that S. muricatum is a sister species to members of sections Petota + Lycopersicum + Etuberosum. We expect that this first sweet cucumber chloroplast genome will provide potential molecular markers and genomic resources to shed light on the genetic diversity and population studies of S. muricatum, which will allow us to identify varieties and ecotypes. Finally, the features and the structural differentiation will provide us with information about the genes of interest, generating tools for the most precise selection of the best individuals of sweet cucumber, in less time and with fewer resources.
An Updated List of Rock Partridge (Alectoris graeca) Haplotypes from the Apennines—Central Italy
Authors: Brustenga Leonardo , Viola Paolo , Girotti Pedro , Amici Andrea , Rossetti Alessandro , Chiesa Stefania , Primi Riccardo , Esposito Luigi , Lucentini Livia
Published: September 2022

We report an updated and expanded list of Rock Partridge (Alectoris graeca) haplotypes found in wild animals throughout the Apennines of central Italy. Samples were collected and identified during a monitoring program of autochthonous Galliformes and from a private collection. The haplotypes were identified on a longer fragment of the mitochondrial control region (D-loop) based on previously reported haplotypes. This novel evidence, based on a wider sampling area and a higher number of analyzed specimens, will be of relevance in both conservation projects and gamebird breeding for restock, as imposed by the Italian Action Plan. Studying longer fragments can also be useful for phylogeographic analysis.
Clogmia albipunctata (Nematocera; Psychodidae) as the Etiologic Agent of Myiasis: True or False?
Authors: Akhoundi Mohammad , Ranorohasimanana Nambininiavo Marianne , Brun Sophie , Kauffmann-Lacroix Catherine , Izri Arezki
Published: September 2022

Clogmia albipunctata, known as drain fly, is a non-hematophagous insect of the Psychodidae family with worldwide distribution, particularly in tropical and temperate areas. It can be found near sewer drains, sewage treatment plants, plant pots, swamps, and any other place containing decaying or moist organic matter. It has been introduced in several publications as the causative agent of myiasis in humans. A case presentation, together with a compilation of findings from a database, including 51 scientific publications in the literature, allowed us to overview critically in detail the variable aspects of epidemiology, life cycle, biology, and medical importance of this insect and its probable role in human myiasis. The absence of a precise definition of myiasis and the lack of incontestable epidemiological, entomological, and clinical evidence in the articles introducing C. albipunctata as a causative agent led us to interrogate its role in human myiasis. It is necessary to take into account this misinterpretation and make an accurate diagnosis based on the isolation of insect larvae from the corresponding lesion.
Predicting Placenta Accreta Spectrum Disorders in a Cohort of Pregnant Patients in the North-East Region of Romania—Diagnostic Accuracy of Ultrasound and Magnetic Resonance Imaging
Authors: Haba Raluca Maria , Pristavu Anda Ioana , Cobzeanu Maria-Luiza , Carauleanu Alexandru , Sadiye Scripcariu Ioana , Vasilache Ingrid Andrada , Minciuna Dorina Adelina , Negru Dragos , Socolov Demetra Gabriela
Published: September 2022

Background: Placenta accreta spectrum (PAS) disorders are associated with high mortality and morbidity due to postpartum hemorrhage, hysterectomy, and organ injury, and a multidisciplinary team is required for an individualized case management. In this study, we assessed the diagnostic and prognostic accuracy of the most important ultrasonographic (US) and magnetic resonance imagining (MRI) markers for PAS disorders. Material and Methods: The study included 39 adult pregnant patients with at least one previous cesarean delivery and both US and MRI investigations for placenta previa evaluated at the tertiary maternity hospital ‘Cuza Voda’, Iasi, between 2019 and 2021. The following US signs were evaluated: intra-placental lacunae, loss of the retroplacental hypoechoic zone, myometrial thinning < 1 mm, bladder wall interruption, placental bulging, bridging vessels, and the hypervascularity of the uterovesical or retroplacental space. The MRI signs that were evaluated were intra-placental dark T2 bands, placental bulging, loss of the retroplacental hypointense line on T2 images, myometrial thinning, bladder wall interruption, focal exophytic placental mass, and abnormal vascularization of the placental bed. Results: The US and MRI signs analyzed in our study presented adequate sensitivities and specificities for PAS, but no sign proved to be a useful predictor by itself. The presence of three or more US markers for accretion was associated with a sensitivity of 84.6.6% and a specificity of 92.3% (p < 0.001). The presence of three or more MRI signs supplemented these results and were associated with a sensitivity of 92.3% and a specificity of 61.5% for predicting PAS (p < 0.001). Moreover, US and MRI findings were correlated with FIGO grading and severity of PAS. Conclusions: Even though no US or MRI finding alone can predict PAS with high sensitivity and specificity, our study proves that the presence of three or more imagistic signs could significantly increase the diagnostic accuracy of this condition. Furthermore, US and MRI could be useful tools for evaluating prognostic and perinatal planning.
Neuromuscular Stability of Dental Occlusion in Patients Treated with Aligners and Fixed Orthodontic Appliance: A Preliminary Electromyographical Longitudinal Case-Control Study
Authors: Dellavia Claudia Paola Bruna , Begnoni Giacomo , Zerosi Cristiana , Guenza Guia , Khomchyna Natalie , Rosati Riccardo , Musto Federica , Pellegrini Gaia
Published: September 2022

The aim of the present study was to evaluate if, after treatment with aligners (ALIGN) and fixed orthodontic appliance (FOA), alterations of the neuromuscular activity may occur and if differences in these changes can be detected between the two treatments. Sixteen healthy patients (7 FOA, 9 ALIGN) with class I or class II molar relation were recruited. Standardized surface electromyography (ssEMG) was used to evaluate the activity of the masticatory muscles (masseters-MM and temporalis-TM) before the beginning of the orthodontic treatment (T1), at the end (T2), and 3 months (T3) after the end of the treatment. Intragroup (within timepoints) and intergroup differences were statistically analyzed. At T1, the mean values of each ssEMG index were within the normal range in both groups. At T2, the FOA group showed larger differential recruitment of the MM than TA muscles with a value slightly over the normal range. All the indexes were normalized at T3, and no differences emerged between groups. In the FOA group, the index of MM symmetrical contraction increased significantly at T3 compared to T1 and T2. In the ALIGN group, no significant changes were observed between each timepoint. In FOA subjects, a slight alteration of the muscular activity appeared immediately after bracket removal and this alteration normalized after 3 months of rescue. In subjects treated with aligners, no significant alteration of the muscular activity was assessed.
Scoping Review Protocol of Technological Interventions for Vocational Inclusion of Individuals with Disabilities
Authors: Hamideh Kerdar Sara , Kirchhoff Britta Marleen , Bächler Liane , Adolph Lars
Published: September 2022

Technology could improve the vocational inclusion of people with disabilities, thus increasing their wellbeing and competence development. Moreover, societies could benefit from their skills and expertise. In this protocol, the objectives, structure, and further details of a scoping review on the subject of the vocational inclusion of people with disabilities via technologies are described. This article additionally demonstrates how a piloting phase can be used for the further development of the protocol. The focus of the proposed scoping review is disability, technology, and task/work. Expansive and specific keywords will be searched in APA PsycInfo, APA PsycArticles, and CINAHL Complete via EBSCOhost, Web of Science, Embase, Scopus, and IEEE Xplore. As regards the grey literature, ProQuest will be used for dissertations and theses and Google Scholar will be hand searched. Articles published in 2012–2022 focused on working-age adults will be exported to EndNote and titles/abstracts will be monitored. We further describe the inclusion and exclusion criteria, data extraction, and charting strategies of the proposed scoping review. The results will be mapped and reported based on disability, technology, and task. For the improvement of the protocol, a pilot study in February 2022 was performed. The results from the pilot, briefly reported herein, led to a transparent and clear structure of the proposed scoping review.