bluuebunny/crossref_metadata_2025 huggingface dataset:
Tryptophan Ameliorates Barrier Integrity and Alleviates the Inflammatory Response to Enterotoxigenic Escherichia coli K88 Through the CaSR/Rac1/PLC-γ1 Signaling Pathway in Porcine Intestinal Epithelial Cells
Authors: Liu Guangmang , Gu Ke , Wang Fang , Jia Gang , Zhao Hua , Chen Xiaoling , Wu Caimei , Zhang Ruinan , Tian Gang , Cai Jingyi , Tang Jiayong , Wang Jing
Published: October 2021

Background

Impaired intestinal barrier integrity plays a crucial role in the development of many diseases such as obesity, inflammatory bowel disease, and type 2 diabetes. Thus, protecting the intestinal barrier from pathological disruption is of great significance. Tryptophan can increase gut barrier integrity, enhance intestinal absorption, and decrease intestinal inflammation. However, the mechanism of tryptophan in decreasing intestinal barrier damage and inflammatory response remains largely unknown. The objective of this study was to test the hypothesis that tryptophan can enhance intestinal epithelial barrier integrity and decrease inflammatory response mediated by the calcium-sensing receptor (CaSR)/Ras-related C3 botulinum toxin substrate 1 (Rac1)/phospholipase Cγ1 (PLC-γ1) signaling pathway.

Methods

IPEC-J2 cells were treated with or without enterotoxigenic Escherichia coli (ETEC) K88 in the absence or presence of tryptophan, CaSR inhibitor (NPS-2143), wild-type CaSR overexpression (pcDNA3.1-CaSR-WT), Rac1-siRNA, and PLC-γ1-siRNA.

Results

The results showed that ETEC K88 decreased the protein concentration of occludin, zonula occludens-1 (ZO-1), claudin-1, CaSR, total Rac1, Rho family member 1 of porcine GTP-binding protein (GTP-rac1), phosphorylated phospholipase Cγ1 (p-PLC-γ1), and inositol triphosphate (IP3); suppressed the transepithelial electrical resistance (TEER); and enhanced the permeability of FITC-dextran compared with the control group. Compared with the control group, 0.7 mM tryptophan increased the protein concentration of CaSR, total Rac1, GTP-rac1, p-PLC-γ1, ZO-1, claudin-1, occludin, and IP3; elevated the TEER; and decreased the permeability of FITC-dextran and contents of interleukin-8 (IL-8) and TNF-α. However, 0.7 mM tryptophan+ETEC K88 reversed the effects induced by 0.7 mM tryptophan alone. Rac1-siRNA+tryptophan+ETEC K88 or PLC-γ1-siRNA+tryptophan+ETEC K88 reduced the TEER, increased the permeability of FITC-dextran, and improved the contents of IL-8 and TNF-α compared with tryptophan+ETEC K88. NPS2143+tryptophan+ETEC K88 decreased the TEER and the protein concentration of CaSR, total Rac1, GTP-rac1, p-PLC-γ1, ZO-1, claudin-1, occludin, and IP3; increased the permeability of FITC-dextran; and improved the contents of IL-8 and TNF-α compared with tryptophan+ETEC K88. pcDNA3.1-CaSR-WT+Rac1-siRNA+ETEC K88 and pcDNA3.1-CaSR-WT+PLC-γ1-siRNA+ETEC K88 decreased the TEER and enhanced the permeability in porcine intestine epithelial cells compared with pcDNA3.1-CaSR-WT+ETEC K88.

Conclusion

Tryptophan can improve intestinal epithelial barrier integrity and decrease inflammatory response through the CaSR/Rac1/PLC-γ1 signaling pathway.
Rapid and Sensitive Detection of Vibrio vulnificus Using CRISPR/Cas12a Combined With a Recombinase-Aided Amplification Assay
Authors: Xiao Xingxing , Lin Ziqin , Huang Xianhui , Lu Jinfang , Zhou Yan , Zheng Laibao , Lou Yongliang
Published: October 2021

Vibrio vulnificus is an important zoonotic and aquatic pathogen and can cause vibriosis in humans and aquatic animals (especially farmed fish and shrimp species). Rapid and sensitive detection methods for V. vulnificus are still required to diagnose human vibriosis early and reduce aquaculture losses. Herein, we developed a rapid and sensitive diagnostic method comprising a recombinase-aided amplification (RAA) assay and the CRISPR/Cas12a system (named RAA-CRISPR/Cas12a) to detect V. vulnificus. The RAA-CRISPR/Cas12a method allows rapid and sensitive detection of V. vulnificus in 40 min without a sophisticated instrument, and the limit of detection is two copies of V. vulnificus genomic DNA per reaction. Meanwhile, the method shows satisfactory specificity toward non-target bacteria and high accuracy in the spiked blood, stool, and shrimp samples. Therefore, our proposed rapid and sensitive V. vulnificus detection method, RAA-CRISPR/Cas12a, has great potential for early diagnosis of human vibriosis and on-site V. vulnificus detection in aquaculture and food safety control.
Transcriptomic Profiling Reveals the Antiapoptosis and Antioxidant Stress Effects of Fos in Ischemic Stroke
Authors: Mu Qiancheng , Zhang Yuxuan , Gu Long , Gerner Stefan T. , Qiu Xiancheng , Tao Qianke , Pang Jinwei , Dipritu Ghosh , Zhang Lifang , Yin Shigang , Jiang Yong , Peng Jianhua
Published: October 2021

Arterial hypertension is considered the most prevalent risk factor for stroke. Both pathophysiologic and clinical data previously acquired suggest a strong correlation between the hemodynamic nature of arterial hypertension and an increase in the risk of ischemic insult to tissues. However, the knowledge of specific molecular interactions between hypertension and ischemic stroke (IS) is limited. In this study, we performed systematic bioinformatics analysis of stroke-prone spontaneous hypertensive brain tissue samples of rats (GSE41452), middle cerebral artery occlusion of brain tissue samples of rats (GSE97537), and peripheral blood array data of IS patients (GSE22255). We identified that Fos, an immediate-early gene (IEG) that responds to alterations in arterial blood pressure, has a strong correlation with the occurrence and prognosis of IS. To further evaluate the potential function of Fos, the oxygen–glucose deprivation model and RNA sequencing of HT22 neuronal cells were performed. Consistent with the sequencing results, real-time quantitative PCR and Western blot indicate that Fos was elevated at 3 h and returned to normal levels at 6 h after oxygen–glucose deprivation. Knock-down of Fos by lentivirus significantly increased the oxidative stress level, neuronal apoptosis, and inhibited the mitochondrial function. In conclusion, Fos acts as an important link between hypertension and IS. Furthermore, Fos can be used as a potential biomarker for target therapy in the prevention of stroke among hypertensive patients and also potential treatment targeting apoptosis and oxidative stress after its onset.
How Does Information Overload Affect Consumers’ Online Decision Process? An Event-Related Potentials Study
Authors: Peng Minjing , Xu Zhicheng , Huang Haiyang
Published: October 2021

One of the advantages of e-retailers is their capability to provide a large amount of information to consumers. However, when the amount of information exceeds consumers’ information processing capacities, it will lead to worse decision quality and experience, causing the information overload effect. In this study, the event-related potentials (ERPs) were applied to examine the hidden neural mechanism of the impact of information overload on consumers’ decision processes. Behavioral data showed that people would spend more time making decisions when faced with information overload. Neurophysiologically, consumers would invest less attentional resources in the high amount of information (HAI) condition than those in the low amount of information (LAI) condition and lead to less positive P2 amplitudes. The HAI condition would increase decision difficulty than would the LAI condition and result in smaller P3 amplitudes. In addition, an increased late positive component (LPC) was observed for the HAI condition in contrast to the LAI condition, indicating that consumers were more inclined to have decision process regret when consumers were overloaded. We further investigated the dynamic information processing when consumers got over information overload by mining the brain’s time-varying networks. The results revealed that during the decision process and the neural response stage, the central area controlled other brain regions’ activities for the HAI condition, suggesting that people may still consider and compare other important information after the decision process when faced with information overload. In general, this study may provide neural evidence of how information overload affects consumers’ decision processes and ultimately damages decision quality.
Pathophysiological Ionotropic Glutamate Signalling in Neuroinflammatory Disease as a Therapeutic Target
Authors: Fairless Richard , Bading Hilmar , Diem Ricarda
Published: October 2021

Glutamate signalling is an essential aspect of neuronal communication involving many different glutamate receptors, and underlies the processes of memory, learning and synaptic plasticity. Despite neuroinflammatory diseases covering a range of maladies with very different biological causes and pathophysiologies, a central role for dysfunctional glutamate signalling is becoming apparent. This is not just restricted to the well-described role of glutamate in mediating neurodegeneration, but also includes a myriad of other influences that glutamate can exert on the vasculature, as well as immune cell and glial regulation, reflecting the ability of neurons to communicate with these compartments in order to couple their activity with neuronal requirements. Here, we discuss the role of pathophysiological glutamate signalling in neuroinflammatory disease, using both multiple sclerosis and Alzheimer’s disease as examples, and how current steps are being made to harness our growing understanding of these processes in the development of neuroprotective strategies. This review focuses in particular on N-methyl-D-aspartate (NMDA) and 2-amino-3-(3-hydroxy-5-methylisooxazol-4-yl) propionate (AMPA) type ionotropic glutamate receptors, although metabotropic, G-protein-coupled glutamate receptors may also contribute to neuroinflammatory processes. Given the indispensable roles of glutamate-gated ion channels in synaptic communication, means of pharmacologically distinguishing between physiological and pathophysiological actions of glutamate will be discussed that allow deleterious signalling to be inhibited whilst minimising the disturbance of essential neuronal function.
Prefrontal Physiomarkers of Anxiety and Depression in Parkinson’s Disease
Authors: de Hemptinne Coralie , Chen Witney , Racine Caroline A. , Seritan Andreea L. , Miller Andrew M. , Yaroshinsky Maria S. , Wang Sarah S. , Gilron Roee , Little Simon , Bledsoe Ian , San Luciano Marta , Katz Maya , Chang Edward F. , Dawes Heather E. , Ostrem Jill L. , Starr Philip A.
Published: October 2021

Objective: Anxiety and depression are prominent non-motor symptoms of Parkinson’s disease (PD), but their pathophysiology remains unclear. We sought to understand their neurophysiological correlates from chronic invasive recordings of the prefrontal cortex (PFC).

Methods: We studied four patients undergoing deep brain stimulation (DBS) for their motor signs, who had comorbid mild to moderate anxiety and/or depressive symptoms. In addition to their basal ganglia leads, we placed a permanent prefrontal subdural 4-contact lead. These electrodes were attached to an investigational pulse generator with the capability to sense and store field potential signals, as well as deliver therapeutic neurostimulation. At regular intervals over 3–5 months, participants paired brief invasive neural recordings with self-ratings of symptoms related to depression and anxiety.

Results: Mean age was 61 ± 7 years, mean disease duration was 11 ± 8 years and a mean Unified Parkinson’s Disease Rating Scale, with part III (UPDRS-III) off medication score of 37 ± 13. Mean Beck Depression Inventory (BDI) score was 14 ± 5 and Beck Anxiety Index was 16.5 ± 5. Prefrontal cortex spectral power in the beta band correlated with patient self-ratings of symptoms of depression and anxiety, with r-values between 0.31 and 0.48. Mood scores showed negative correlation with beta spectral power in lateral locations, and positive correlation with beta spectral power in a mesial recording location, consistent with the dichotomous organization of reward networks in PFC.

Interpretation: These findings suggest a physiological basis for anxiety and depression in PD, which may be useful in the development of neurostimulation paradigms for these non-motor disease features.
Prognostic and Predictive Value of Transcription Factors Panel for Digestive System Carcinoma
Authors: Fang Guoxu , Fan Jianhui , Ding Zongren , Li Rong , Lin Kongying , Fu Jun , Huang Qizhen , Zeng Yongyi , Liu Jingfeng
Published: October 2021

Purpose

Digestive system carcinoma is one of the most devastating diseases worldwide. Lack of valid clinicopathological parameters as prognostic factors needs more accurate and effective biomarkers for high-confidence prognosis that guide decision-making for optimal treatment of digestive system carcinoma. The aim of the present study was to establish a novel model to improve prognosis prediction of digestive system carcinoma, with a particular interest in transcription factors (TFs).

Materials and Methods

A TF-related prognosis model of digestive system carcinoma with data from TCGA database successively were processed by univariate and multivariate Cox regression analyses. Then, for evaluating the prognostic prediction value of the model, ROC curve and survival analysis were performed by external data from GEO database. Furthermore, we verified the expression of TFs expression by qPCR in digestive system carcinoma tissue. Finally, we constructed a TF clinical characteristics nomogram to furtherly predict digestive system carcinoma patient survival probability with TCGA database.

Results

By Cox regression analysis, a panel of 17 TFs (NFIC, YBX2, ZBTB47, ZNF367, CREB3L3, HEYL, FOXD1, TIGD1, SNAI1, HSF4, CENPA, ETS2, FOXM1, ETV4, MYBL2, FOXQ1, ZNF589) was identified to present with powerful predictive performance for overall survival of digestive system carcinoma patients based on TCGA database. A nomogram that integrates TFs was established, allowing efficient prediction of survival probabilities and displaying higher clinical utility.

Conclusion

The 17-TF panel is an independent prognostic factor for digestive system carcinoma, and 17 TFs based nomogram might provide implication an effective approach for digestive system carcinoma patient management and treatment.
RIPK3 and AXL Expression Study in Primary Cutaneous Melanoma Unmasks AXL as Predictor of Sentinel Node Metastasis: A Pilot Study
Authors: Nicolè Lorenzo , Cappello Filippo , Cappellesso Rocco , Piccin Luisa , Ventura Laura , Guzzardo Vincenza , Del Fiore Paolo , Chiarion-Sileni Vanna , Dei Tos Angelo Paolo , Mocellin Simone , Fassina Ambrogio
Published: October 2021

Malignant melanoma (MM) is the most lethal skin cancer. AXL is a tyrosine kinase receptor involved in several oncogenic processes and might play a role in blocking necroptosis (a regulated cell death mechanism) in MM through the downregulation of the necroptotic-related driver RIPK3. The aim of this study was to evaluate the clinical impact of the expression of AXL and RIPK3 in 108 primary cutaneous MMs. Association between AXL and RIPK3 immunoreactivity and clinical–pathological variables, sentinel lymph node status, and tumor-infiltrating lymphocytes (TILs) was assessed. Immunoreaction in tumor cells was detected in 30 cases (28%; range, 5%–80%) and in 17 cases (16%; range, 5%–50%) for AXL and RIPK3, respectively. Metastases in the sentinel lymph nodes were detected in 14 out of 61 patients, and these were associated with AXL-positive immunoreactivity in the primary tumor (p < 0.0001). No association between AXL and TILs was found. RIPK3 immunoreactivity was not associated with any variables. A final logistic regression analysis showed Breslow and AXL-positive immunoreactivity as the stronger predictor for positive sentinel node status [area under the receiver operating characteristic curve (AUC) of 0.96]. AXL could be a potential new biomarker for MM risk assessment, and it deserves to be further investigated in larger studies.
Influence of Clinical and Tumor Factors on Interfraction Setup Errors With Rotation Correction for Vacuum Cushion in Lung Stereotactic Body Radiation Therapy
Authors: Chen Hua , Liu Lingxiang , Wang Hao , Shao Yan , Gu Hengle , Duan Yanhua , Feng Aihui , Huang Ying , Xu Zhiyong
Published: October 2021

Purpose

To explore the influence of clinical and tumor factors over interfraction setup errors with rotation correction for non-small cell lung cancer (NSCLC) stereotactic body radiation therapy (SBRT) patients immobilized in vacuum cushion (VC) to better understand whether patient re-setup could further be optimized with these parameters.

Materials and Methods

This retrospective study was conducted on 142 NSCLC patients treated with SBRT between November 2017 to July 2019 in the local institute. Translation and rotation setup errors were analyzed in 732 cone-beam computed tomography (CBCT) scans before treatment. Differences between groups were analyzed using independent sample t-test. Logistic regression test was used to analyze possible correlations between patient re-setup and clinical and tumor factors.

Results

Mean setup errors were the largest in anterior–posterior (AP) direction (3.2 ± 2.4 mm) compared with superior–inferior (SI) (2.8 ± 2.1 mm) and left–right (LR) (2.5 ± 2.0 mm) directions. The mean values were similar in pitch, roll, and rtn directions. Of the fractions, 83.7%, 90.3%, and 86.6% satisfied setup error tolerance limits in AP, SI, and LR directions, whereas 95% had rotation setup errors of <2° in the pitch, roll, or rtn directions. Setup errors were significantly different in the LR direction when age, body mass index (BMI), and “right vs. left” location parameters were divided into groups. Both univariate and multivariable model analyses showed that age (p = 0.006) and BMI (p = 0.002) were associated with patient re-setup.

Conclusions

Age and BMI, as clinical factors, significantly influenced patient re-setup in the current study, whereas all other clinical and tumor factors were not correlated with patient re-setup. The current study recommends that more attention be paid to setup for elderly patients and patients with larger BMI when immobilized using VC, especially in the left–right direction.
Mutual Information-Based Non-Local Total Variation Denoiser for Low-Dose Cone-Beam Computed Tomography
Authors: Lee Ho , Sung Jiwon , Choi Yeonho , Kim Jun Won , Lee Ik Jae
Published: October 2021

Conventional non-local total variation (NLTV) approaches use the weight of a non-local means (NLM) filter, which degrades performance in low-dose cone-beam computed tomography (CBCT) images generated with a low milliampere-seconds (mAs) parameter value because a local patch used to determine the pixel weights comprises noisy-damaged pixels that reduce the similarity between corresponding patches. In this paper, we propose a novel type of NLTV based on a combination of mutual information (MI): MI-NLTV. It is based on a statistical measure for a similarity calculation between the corresponding bins of non-local patches vs. a reference patch. The weight is determined in terms of a statistical measure comprising the MI value between corresponding non-local patches and the reference-patch entropy. The MI-NLTV denoising process is applied to CBCT images generated by the analytical reconstruction algorithm using a ray-driven backprojector (RDB). The MI-NLTV objective function is minimized based on the steepest gradient descent optimization to augment the difference between a real structure and noise, cleaning noisy pixels without significant loss of the fine structure and details that remain in the reconstructed images. The proposed method was evaluated using patient data and actual phantom measurement data acquired with lower mAs. The results show that integrating the RDB further enhances the MI-NLTV denoising-based analytical reconstruction algorithm to achieve a higher CBCT image quality when compared with those generated by NLTV denoising-based approach, with an average of 15.97% higher contrast-to-noise ratio, 2.67% lower root mean square error, 0.12% lower spatial non-uniformity, 1.14% higher correlation, and an average of 18.11% higher detectability index. These quantitative results indicate that the incorporation of MI makes the NLTV more stable and robust than the conventional NLM filter for low-dose CBCT imaging. In addition, achieving clinically acceptable CBCT image quality despite low-mAs projection acquisition can reduce the burden on common online CBCT imaging, improving patient safety throughout the course of radiotherapy.
The Role of Radiotherapy in Soft Tissue Sarcoma on Extremities With Lymph Nodes Metastasis: An IPTW Propensity Score Analysis of the SEER Database
Authors: Qiu Xinzhu , He Hongbo , Zeng Hao , Tong Xiaopeng , Liu Qing
Published: October 2021

Background

Soft tissue sarcomas on extremities with regional lymph nodes metastasis (STSE-RLNM) is a devastating situation. Optimizing therapeutic approaches is vital but hampered by a shortage of randomized trials. We used a population-level database to evaluate radiotherapy’s impact on sarcoma-specific survival (SSS) and overall survival (OS) for surgery for STSE-RLNM.

Methods

We retrospectively screened data from the SEER database (2004–2015), and 265 patients with STSE-RLNM who received surgery, with (134) or without (131) radiotherapy, were enrolled in this study. A propensity-score-matched analysis with the inverse probability of treatment weighting (IPTW) Kaplan–Meier curve was created. The log-rank test and Cox regression analysis were performed to compare SSS and OS in patients with and without radiotherapy. Further analysis of radiotherapy time was conducted, and the Kaplan–Meier curve and the log-rank test were done. Landmark analysis was introduced to attenuate the immortal bias.

Results

In the original unadjusted cohort, the radiotherapy + surgery group is associated with improved SSS [hazard ratio (HR), 0.66; 95% CI, 0.47–0.91; p = 0.011] and OS (HR, 0.64; 95% CI, 0.47–0.88; p = 0.006). This significant treatment effect was also noted in IPTW-adjusted Cox regression either on SSS (HR, 0.65; 95% CI, 0.45–0.93; p = 0.020) or on OS (HR, 0.64; 95% CI, 0.46–0.91; p = 0.013). The Kaplan–Meier curve and log-rank test showed that pre- and postoperative radiotherapy was not related to SSS (p = 0.980 or OS (p = 0.890).

Conclusion

Radiotherapy and surgery has a significant benefit on the prognosis of patients with STSE-RLNM compared to surgery alone. These findings should be considered when making treatment decisions for them.
Alternative Energy: Breaking Down the Diverse Metabolic Features of Lung Cancers
Authors: Cargill Kasey R. , Hasken William L. , Gay Carl M. , Byers Lauren A.
Published: October 2021

Metabolic reprogramming is a hallmark of cancer initiation, progression, and relapse. From the initial observation that cancer cells preferentially ferment glucose to lactate, termed the Warburg effect, to emerging evidence indicating that metabolic heterogeneity and mitochondrial metabolism are also important for tumor growth, the complex mechanisms driving cancer metabolism remain vastly unknown. These unique shifts in metabolism must be further investigated in order to identify unique therapeutic targets for individuals afflicted by this aggressive disease. Although novel therapies have been developed to target metabolic vulnerabilities in a variety of cancer models, only limited efficacy has been achieved. In particular, lung cancer metabolism has remained relatively understudied and underutilized for the advancement of therapeutic strategies, however recent evidence suggests that lung cancers have unique metabolic preferences of their own. This review aims to provide an overview of essential metabolic mechanisms and potential therapeutic agents in order to increase evidence of targeted metabolic inhibition for the treatment of lung cancer, where novel therapeutics are desperately needed.
Gastrointestinal Cancers and Liver Cirrhosis: Implications on Treatments and Prognosis
Authors: Xiang Ze , Li Yiqi , Zhu Chaojie , Hong Tu , He Xianglin , Zhu Hua , Jiang Danbin
Published: October 2021

Liver cirrhosis tends to increase the risk in the management of gastrointestinal tumors. Patients with gastrointestinal cancers and liver cirrhosis often have serious postoperative complications and poor prognosis after surgery. Multiple studies have shown that the stage of gastrointestinal cancers and the grade of cirrhosis can influence surgical options and postoperative complications. The higher the stage of cancer and the poorer the degree of cirrhosis, the less the surgical options and the higher the risk of postoperative complications. Therefore, in the treatment of patients with gastrointestinal cancer and liver cirrhosis, clinicians should comprehensively consider the cancer stage, cirrhosis grade, and possible postoperative complications. This review summarizes the treatment methods of patients with different gastrointestinal cancer complicated with liver cirrhosis.
Acute Mountain Sickness Following Incremental Trekking to High Altitude: Correlation With Plasma Vascular Endothelial Growth Factor Levels and the Possible Effects of Dexamethasone and Acclimatization Following Re-exposure
Authors: Winter Craig , Bjorkman Tracy , Miller Stephanie , Nichols Paul , Cardinal John , O’Rourke Peter , Ballard Emma , Nasrallah Fatima , Vegh Viktor
Published: October 2021

Purpose: The recognition and treatment of high-altitude illness (HAI) is increasingly important in global emergency medicine. High altitude related hypobaric hypoxia can lead to acute mountain sickness (AMS), which may relate to increased expression of vascular endothelial growth factor (VEGF), and subsequent blood-brain barrier (BBB) compromise. This study aimed to establish the relationship between AMS and changes in plasma VEGF levels during a high-altitude ascent. VEGF level changes with dexamethasone, a commonly used AMS medication, may provide additional insight into AMS.

Methods: Twelve healthy volunteers ascended Mt Fuji (3,700 m) and blood samples were obtained at distinct altitudes for VEGF analysis. Oxygen saturation (SPO2) measurements were also documented at the same time-point. Six out of the 12 study participants were prescribed dexamethasone for a second ascent performed 48 h later, and blood was again collected to establish VEGF levels.

Results: Four key VEGF observations could be made based on the data collected: (i) the baseline VEGF levels between the two ascents trended upwards; (ii) those deemed to have AMS in the first ascent had increased VEGF levels (23.8–30.3 pg/ml), which decreased otherwise (23.8–30.3 pg/ml); (iii) first ascent AMS participants had higher VEGF level variability for the second ascent, and similar to those not treated with dexamethasone; and (iv) for the second ascent dexamethasone participants had similar VEGF levels to non-AMS first ascent participants, and the variability was lower than for first ascent AMS and non-dexamethasone participants. SPO2 changes were unremarkable, other than reducing by around 5% irrespective of whether measurement was taken for the first or second ascent.

Conclusion: First ascent findings suggest a hallmark of AMS could be elevated VEGF levels. The lack of an exercise-induced VEGF level change strengthened the notion that elevated plasma VEGF was brain-derived, and related to AMS.
Dear Computer on My Desk, Which Candidate Fits Best? An Assessment of Candidates’ Perception of Assessment Quality When Using AI in Personnel Selection
Authors: Schick Jessica , Fischer Sebastian
Published: October 2021

Recently, with the increase in technological capabilities and the need to reduce bias in candidate selection processes, artificial intelligence (AI)-based selection procedures have been on the rise. However, the literature indicates that candidate reactions to a selection process need to be considered by organizations that compete for employees. In this study, we investigate reactions to AI-based selection procedures in a three-dimensional vignette study among young adults in Germany. By investigating the effects of the dimensions of AI complexity, intangibility, and reliability on the perceived quality of assessment of potential candidates, we found that AI complexity and intangibility impact the perceived quality of assessment negatively when the candidates’ knowledge, strengths, and weaknesses should be assessed. We also found interactive relationships of all three dimensions for the assessment of motivation. In sum, results indicate that candidates are skeptical toward the assessment quality of AI-intense selection processes, especially if these assess complex assessment criteria such as personality or a job performance forecast. Hence, organizations need to be careful when implementing AI-based selection procedures. HR implications are made on the basis of these results to cope with negative candidate perceptions.
Risk Factors for Delirium in the Palliative Care Population: A Systematic Review and Meta-Analysis
Authors: Guo Duan , Lin Taiping , Deng Chuanyao , Zheng Yuxia , Gao Langli , Yue Jirong
Published: October 2021

Objective: Delirium is common and highly distressing for the palliative care population. Until now, no study has systematically reviewed the risk factors of delirium in the palliative care population. Therefore, we performed a systematic review and meta-analysis to evaluate delirium risk factors among individuals receiving palliative care.

Methods: We systematically searched PubMed, Medline, Embase, and Cochrane database to identify relevant observational studies from database inception to June 2021. The methodological quality of the eligible studies was assessed by the Newcastle Ottawa Scale. We estimated the pooled adjusted odds ratio (aOR) for individual risk factors using the inverse variance method.

Results: Nine studies were included in the review (five prospective cohort studies, three retrospective case-control studies and one retrospective cross-section study). In pooled analyses, older age (aOR: 1.02, 95% CI: 1.01–1.04, I2 = 37%), male sex (aOR:1.80, 95% CI: 1.37–2.36, I2 = 7%), hypoxia (aOR: 0.87, 95% CI: 0.77–0.99, I2 = 0%), dehydration (aOR: 3.22, 95%CI: 1.75–5.94, I2 = 18%), cachexia (aOR:3.40, 95% CI: 1.69–6.85, I2 = 0%), opioid use (aOR: 2.49, 95%CI: 1.39–4.44, I2 = 0%), anticholinergic burden (aOR: 1.18, 95% CI: 1.07–1.30, I2 = 9%) and Eastern Cooperative Oncology Group Performance Status (aOR: 2.54, 95% CI: 1.56–4.14, I2 = 21%) were statistically significantly associated with delirium.

Conclusion: The risk factors identified in our review can help to highlight the palliative care population at high risk of delirium. Appropriate strategies should be implemented to prevent delirium and improve the quality of palliative care services.
Educator Practices and Perceptions of Integrating Sustainability and Food Systems Concepts Into Elementary Education: Comparative Case Study in Two Northwestern States in the United States
Authors: Stewart Alyssa L. , Ahmed Selena , Warne Teresa , Byker Shanks Carmen , Arnold Shannon
Published: October 2021

Elementary education can equip future consumers and leaders with the systems thinking skills, real-world experiences, and knowledge to make decisions and lead progress toward sustainability transitions. The implementation of learning standards that focus on sustainability is one approach for integrating sustainability and food systems content into elementary education. The purpose of this study was to administer a survey with elementary-level educators to: (1) identify practices and perceptions of integrating sustainability and food systems concepts into the classroom; and (2) determine if practices and perceptions vary based on the presence of state learning standards focused on sustainability. A total of 171 educators completed the majority of the survey from two northwestern states in the United States: Washington (which has state learning standards focused on sustainability) and Montana (which does not have sustainability learning standards). Findings that 30% or less of the surveyed educators integrate sustainability and food systems content in their classroom highlights the urgent need for reforming elementary school curriculum to integrate sustainability as a central unifying framework to support societal and planetary health. Given the similarities in survey responses between educators in Washington and Montana, findings emphasize that state learning standards focused on sustainability are not adequate on their own to foster teacher adoption of sustainability content. There is thus a need for larger curriculum reformation to integrate sustainability as a framework, development of place-based teacher resources, and open access professional development to ensure that elementary-school students cultivate the systems thinking skills, real world experience, and knowledge that will allow them to develop the competencies to ultimately guide society toward meeting the Sustainable Development Goals of the United Nations.
Expression of TSP50, SERCA2 and IL-8 in Colorectal Adenoma and Carcinoma: Correlation to Clinicopathological Factors
Authors: Youssef Heba M. K. , Radi Dina A. , Abd El-Azeem Marwa A.
Published: October 2021

Background:Colorectal cancer (CRC) is the third most common type of cancer, it is considered a genetically heterogeneous disease with different molecular pathways being involved in its initiation and progression. Testes-specific protease 50 (TSP50) gene is a member of cancer/testis antigens that encodes for threonine protease enzyme. Overexpression of TSP50 was found to enhance the progression and invasion of breast cancer and other malignant tumors. SERCA2 is widely expressed in several body tissues; its aberrant expression has been involved in many cancers. IL-8 is an inflammatory cytokine. Alongside its role in inflammation, its expression was reported to induce the migration of tumor cells.

Aim:Study the expression of TSP50, SERCA2 and IL-8 in colorectal adenoma (CRA), CRC and normal colonic tissues to compare the expression of these biomarkers in relation to clinicopathological parameters and prognostic factors.

Results:TSP50, SERCA2 and IL-8 expression varied between normal colonic tissues, CRA and CRC. Significant statistical association was detected between the three biomarkers’ overexpression and degree of dysplasia in CRA. Also, significant statistical relation was found between the three biomarkers’ overexpression and presence of lympho-vascular invasion, advanced TNM staging and high intra-tumoral inflammatory infiltrate. Multivariable analysis showed that the overexpression of the three biomarkers is significantly associated with worse prognosis.

Conclusion:The expression of TSP50, SERCA2 and IL-8 was different between the normal tissue and neoplastic colorectal tissue on one hand and between CRA and CRC on the other. Increased expression of these biomarkers in neoplastic epithelial cells of colorectal carcinoma is associated with adverse prognostic factors and could be considered as independent prognostic factors.
Control of an Electric Vehicle Hybrid Energy Storage System
Authors: Ferencz János , Kelemen András , Imecs Mária
Published: October 2021

In electric vehicles battery life can be prolonged by using hybrid energy storage systems (HESS ), which combine high energy density batteries with supercapacitors, characterized by high power density. This paper deals with the control of electronic power converters from an active parallel HESS. The load of the HESS is the electrical motor drive of an electric vehicle. The interfaces between the DC-link and the power sources are four-phase bidirectional DC-DC converters driven in current control mode, based on the current references supplied by an active parallel HESS power distribution algorithm. We present a rule-based fuzzy energy management algorithm for a HESS powered electric vehicle and its simulation in MATLAB/Simulink® environment using the Quasi-Static Simulation (QSS ) and Fuzzy Logic toolboxes. Also, simulation results in driving and regenerative braking operation modes of the electric vehicle are presented.
Effects of Bias-Corrected Regional Climate Projections and Their Spatial Resolutions on Crop Model Results under Different Climatic and Soil Conditions in Austria
Authors: Thaler Sabina , Formayer Herbert , Kubu Gerhard , Trnka Miroslav , Eitzinger Josef
Published: October 2021

The quality, reliability, and uncertainty of Austrian climate projections (ÖKS15) and their impacts on the results of the crop model DSSAT for three different orographic and climatic agricultural regions in Austria were analyzed. Cultivar-specific grain yields of winter wheat, spring barley, and maize were simulated for different soil classes to address three main objectives. First, the uncertainties of simulated crop yields related to the ÖKS15 projections were analyzed under current climate conditions. The climate projections revealed that the case study regions with higher humidity levels generally had lower yield deviations than the drier regions (yield deviations from −19% to +15%). Regarding the simulated crop types, spring barley was found to be less sensitive to the climate projections than rainfed maize, and the response was greater in regions with a low soil water storage capacity. The second objective was to simulate crop yields for the same cultivars using future climate projections. Winter wheat and spring barley tended to show increased yields by the end of the century due to an assumed CO2-fertilization effect in the range of 3–23%, especially under RCP 8.5. However, rainfed and irrigated maize were associated with up to 17% yield reductions in all three study regions due to a shortened growth period caused by warming. The third objective addressed the effects of crop model weather input data with different spatial resolutions (1 vs. 5, 11, and 21 km) on simulated crop yields using the climate projections. Irrigated grain maize and rainfed spring barley had the lowest simulated yield deviations between the spatial scales applied due to their better water supply conditions. The ranges of uncertainty revealed by the different analyses suggest that impact models should be tested with site representative conditions before being applied to develop site-specific adaptation options for Austrian crop production.
Bio-Compost-Based Integrated Soil Fertility Management Improves Post-Harvest Soil Structural and Elemental Quality in a Two-Year Conservation Agriculture Practice
Authors: Jahangir Mohammad Mofizur Rahman , Islam Shanta , Nitu Tazbeen Tabara , Uddin Shihab , Kabir Abul Kalam Mohammad Ahsan , Meah Mohammad Bahadur , Islam Rafiq
Published: October 2021

The impacts of integrated soil fertility management (ISFM) in conservation agriculture need short-term evaluation before continuation of its long-term practice. A split-split plot experiment with tillage (minimum tillage, MT vs. conventional tillage, CT) as the main plot, residue (20% residue, R vs. no residue as a control, NR) as the sub-plot, and compost (Trichocompost, LC; bio-slurry, BS; and recommended fertilization, RD) as the sub-sub plot treatment was conducted for two consecutive years. Composite soils were collected after harvesting the sixth crop of an annual mustard-rice-rice rotation to analyze for nutrient distribution and soil structural stability. The LC increased rice equivalent yield by 2% over RD and 4% over BS, and nitrogen (N) uptake by 11% over RD and 10% over BS. Likewise, LC had higher soil organic carbon (SOC), N, and available sulphur (S) than BS and RD. Conversion of CT to MT reduced rice equivalent yield by 11%, N uptake by 26%, and N-use efficiency by 28%. Conversely, soil structural stability and elemental quality was greater in MT than in CT, indicating the potential of MT to sequester C, N, P, and S in soil aggregates. Residue management increased rice yield in the second year by 4% and corresponding N uptake by 8%. While MT reduced the yield, our results suggest that ISFM with Trichocompost and residue retention under MT improves soil fertility and physical stability to sustain crop productivity.
DiaMOS Plant: A Dataset for Diagnosis and Monitoring Plant Disease
Authors: Fenu Gianni , Malloci Francesca Maridina
Published: October 2021

The classification and recognition of foliar diseases is an increasingly developing field of research, where the concepts of machine and deep learning are used to support agricultural stakeholders. Datasets are the fuel for the development of these technologies. In this paper, we release and make publicly available the field dataset collected to diagnose and monitor plant symptoms, called DiaMOS Plant, consisting of 3505 images of pear fruit and leaves affected by four diseases. In addition, we perform a comparative analysis of existing literature datasets designed for the classification and recognition of leaf diseases, highlighting the main features that maximize the value and information content of the collected data. This study provides guidelines that will be useful to the research community in the context of the selection and construction of datasets.
Pathogenic Variability of the Jackfruit-Bronzing Bacterium Pantoea stewartii Subspecies stewartii Infection to Jackfruit Varieties and Its Pivotal Plant Hosts in Malaysia
Authors: Abidin Nuraizat , Ismail Siti Izera , Vadamalai Ganesan , Yusof Mohd Termizi , Hakiman Mansor , Karam Daljit Singh , Zulperi Dzarifah
Published: October 2021

Infection with Pantoea stewartii subspecies stewartii, which causes jackfruit-bronzing disease, is a huge problem faced by the jackfruit industry in Malaysia. This study was carried out to assess the disease ratings and aggressiveness performance of 28 Pantoea stewartii subspecies stewartii isolated from jackfruit-bronzing diseased jackfruits from four collection areas (Jenderam in Selangor State, Maran and Muadzam Shah in Pahang State, and Ipoh in Perak State) in Peninsular Malaysia, inoculated into jackfruit varieties (Tekam Yellow J33, Hong J34 and Subang Chap Boy J39), the sweetcorn variety, Mas Madu (two-week-old seedlings and nine-week-old seedlings), the cucumber variety, Rocky, and the pineapple variety, MD2. The results revealed that Pantoea stewartii subspecies stewartii produced symptoms upon all inoculations in the pathogenicity testing, thus fulfilling Koch’s postulates, except in the case of J39 and sweetcorn (two-week-old seedlings). No disease symptoms (disease rating 0) were observed in J39 and sweetcorn (two-week-old seedlings) within 14 days post-inoculation (14 dpi). The disease progression (based on disease ratings) proved that the jackfruit variety J39 was the most resistant, whereas J33 and J34 were susceptible to jackfruit-bronzing disease. The disease ratings of the 14-dpi period revealed a variability of disease progression among the 28 bacterial isolates, where the isolate JEN-14 had the fastest and highest disease ratings when inoculated into J33, J34, nine-week-old sweetcorn seedlings, cucumber, and pineapple. Likewise, the AUDPC value, based on disease rating, across the 28 isolates indicated that JEN-14 is the most aggressive and significant of the isolates (J33, J34, nine-week-old sweetcorn seedlings, cucumber, and cucumber pineapple inoculation; p < 0.05). Even though isolates from Jenderam (other than JEN-14) and Maran had better disease ratings and the area under the disease progress curve (AUDPC) values than isolates from Muadzam Shah and Ipoh, no significant differences were found among the isolates (p < 0.05). Based on our findings, we identified the isolate JEN-14 as the best potential candidate to assist in jackfruit-bronzing disease resistance breeding in the future. Last but not least, the methods, disease ratings, and variations of the aggressiveness profiles among the isolates from this study may be beneficial and significant by providing disease-rating references and appropriate screening approaches when selecting the most appropriately aggressive isolates for evaluating the defense response in the disease resistance breeding program.
Molecular Characterization of Blastocystis sp. in Camelus bactrianus in Northwestern China
Authors: Yang Xin , Li Yunhui , Wang Yuxin , Wang Junwei , Lai Peng , Li Yuan , Song Junke , Qi Meng , Zhao Guanghui
Published: October 2021

Blastocystis sp. is an important zoonotic protist in humans and various animals with worldwide distribution. However, there have been no data on the occurrence of Blastocystis sp. in C. bactrianus, an important economic animal in northwestern China. In the present study, a PCR-sequencing tool based on the SSU rRNA gene was applied to investigate the prevalence and genetic diversity of Blastocystis sp. in 638 faecal samples from C. bactrianus in 21 sampling sites within three main breeding areas (Gansu, Inner Mongolia and Xinjiang) in northwestern China. The total prevalence of Blastocystis sp. was 21.8% (139/638) in C. bactrianus, with the infection rates of 29.5% (18/61), 50.0% (14/28) and 19.5% (107/549) for animals aged <2 years, 2–6 years and >6 years, respectively. Significant differences in prevalence were detected among C. bactrianus from three geographic areas (χ2 = 19.972, df = 2, p < 0.001) and all sampling sites (χ2 = 104.154, df = 20, p < 0.001). A total of 16 of 21 sampling sites were positive for Blastocystis sp., with the prevalence ranging from 7.7% to 70.6%. Sequence analysis of the SSU rRNA gene identified eight subtypes in C. bactrianus in the present study, including seven animal adapted subtypes (ST10, ST14, ST21, ST24, ST25, ST26 and ST30) and one potentially novel subtype, with ST10 being the dominant one. To the best of our knowledge, this study provides the first insight for the occurrence and genetic make-up of Blastocystis sp. in C. bactrianus and contributes to the understanding of the transmission of Blastocystis infection in C. bactrianus in China.
Interactive Rooting Towers and Behavioural Observations as Strategies to Reduce Tail Biting on Conventional Pig Fattening Farms
Authors: Kalies Anne , Baumgartner Johannes , Beyerbach Martin , Stanojlovic Milos , Scholz Tobias , Richter Franziska , von Altrock Alexandra , Hennig-Pauka Isabel
Published: October 2021

Eight pens (25 pigs/pen; n = 200) provided with an interactive straw-filled rooting tower (experimental group) and five pens (25 pigs/pen; n = 125) with a stationary (fixed) tower without straw (control group) were compared within three fattening periods on a conventional farm with fully slatted flooring. The effectiveness of the tower to trigger favourable behaviour in feeding and outside feeding periods was assessed. The incidence of deep tail injuries was lower in the experimental group (experimental group: Odds Ratio 0.3, p < 0.001) and was influenced by the batch (Odds Ratio: 2.38, p < 0.001) but not by pen and sex. In spring, most pens were excluded due to severe tail biting. Tail injury scores were more severe in the control group in weeks 5, 6 and 7 compared to the experimental group (p = 0.002, p < 0.001, p < 0.001, respectively). Tower manipulation was more frequent during feeding compared to outside feeding time (p = 0.002). More head than tail manipulation occurred in the experimental group (p = 0.03). The interactive tower as the only measure was not appropriate to reduce tail biting sufficiently in pigs with intact tails on a conventional fattening farm. Of high priority to prevent tail biting outbreaks was the early detection of biting pigs.