bluuebunny/crossref_metadata_2025 huggingface dataset:
Integrated Multi-Omics Investigations of Metalloproteinases in Colon Cancer: Focus on MMP2 and MMP9
Authors: Buttacavoli Miriam , Di Cara Gianluca , Roz Elena , Pucci-Minafra Ida , Feo Salvatore , Cancemi Patrizia
Published: November 2021

Colorectal cancer (CRC) develops by genetic and epigenetic alterations. However, the molecular mechanisms underlying metastatic dissemination remain unclear and could benefit from multi-omics investigations of specific protein families. Matrix metalloproteinases (MMPs) are proteolytic enzymes involved in ECM remodeling and the processing of bioactive molecules. Increased MMP expression promotes the hallmarks of tumor progression, including angiogenesis, invasion, and metastasis, and is correlated with a shortened survival. Nevertheless, the collective role and the possible coordination of MMP members in CRC are poorly investigated. Here, we performed a multi-omics analysis of MMP expression in CRC using data mining and experimental investigations. Several databases were used to deeply mine different expressions between tumor and normal tissues, the genetic and epigenetic alterations, the prognostic value as well as the interrelationships with tumor immune-infiltrating cells (TIICs). A special focus was placed on to MMP2 and MMP9: their expression was correlated with immune markers and the interaction network of co-expressed genes disclosed their implication in epithelial to mesenchymal transition (EMT) and immune response. Finally, the activity levels of MMP2 and MMP9 in a cohort of colon cancer samples, including tissues and the corresponding sera, was also investigated by zymography. Our findings suggested that MMPs could have a high potency, as they are targeted in colon cancer, and might serve as novel biomarkers, especially for their involvement in the immune response. However, further studies are needed to explore the detailed biological functions and molecular mechanisms of MMPs in CRC, also in consideration of their expression and different regulation in several tissues.
Evaluation of the Binding Mechanism of Human Defensin 5 in a Bacterial Membrane: A Simulation Study
Authors: Awang Tadsanee , Chairatana Phoom , Vijayan Ranjit , Pongprayoon Prapasiri
Published: November 2021

Human α-defensin 5 (HD5) is a host-defense peptide exhibiting broad-spectrum antimicrobial activity. The lipopolysaccharide (LPS) layer on the Gram-negative bacterial membrane acts as a barrier to HD5 insertion. Therefore, the pore formation and binding mechanism remain unclear. Here, the binding mechanisms at five positions along the bacterial membrane axis were investigated using Molecular Dynamics. (MD) simulations. We found that HD5 initially placed at positions 1 to 3 moved up to the surface, while HD5 positioned at 4 and 5 remained within the membrane interacting with the middle and inner leaflet of the membrane, respectively. The arginines were key components for tighter binding with 3-deoxy-d-manno-octulosonic acid (KDO), phosphates of the outer and inner leaflets. KDO appeared to retard the HD5 penetration.
Effectiveness of Different Soft Acaricides against Honey Bee Ectoparasitic Mite Varroa destructor (Acari: Varroidae)
Authors: Qadir Ziyad Abdul , Idrees Atif , Mahmood Rashid , Sarwar Ghulam , Bakar Muhammad Abu , Ahmad Saboor , Raza Muhammad Mohsin , Li Jun
Published: November 2021

Honey bees (Apis mellifera) are essential for their products—honey, royal jelly, pollen, propolis and beeswax. They are also indispensable because they support ecosystems with their pollination services. However, the production and functions of honey bees are hindered by the arthropod pest Varroa destructor, which attacks bees through its feeding activities. Efforts to control varroa mites have been made through the development of various synthetic pesticide groups, but have had limited success because the mites developed resistance and some of these pesticides are harmful to bees. Branded pesticides are rarely used in Pakistan, as beekeepers utilize acaricides from unknown sources. There is a need to create awareness of available naturally occurring acaricides that may serve as an alternative to synthetic acaricides. Although some naturally occurring compounds are considered toxic to the environment, the soft acaricides oxalic acid, thymol, and formic acid 65% are usually safe for honey bee colonies and beekeepers, when handled appropriately. The current study investigated the effectiveness of formic acid (10, 15, and 20 mL/hive), oxalic acid (4.2, 3.2, and 2.1%/hive), and thymol (6, 4, and 2 g/hive) in controlling mite infestation. The results indicated that all treatments significantly reduced the mite population (p < 0.05). The average efficacies of oxalic acid at 3.2% (94.84% ± 0.34) and 4.2% (92.68% ± 0.37) were significantly higher than those of the other treatments. The lowest efficacy was recorded in formic acid 65% at 10 mL (54.13%). Overall, the results indicated that soft acaricides—such as oxalic acid at 3.2% and 4.2% concentrations—are very effective at controlling varroa mites and can be used in broodless conditions without side effects.
Clinical Outcome and Drug Expenses of Intravitreal Therapy for Diabetic Macular Edema: A Retrospective Study in Sardinia, Italy
Authors: Altana Chiara , Donadu Matthew Gavino , Dore Stefano , Boscia Giacomo , Carmelita Gabriella , Zanetti Stefania , Boscia Francesco , Pinna Antonio
Published: November 2021

Background: Diabetic macular edema (DME) is a leading cause of visual loss in working-age adults. The purpose of this retrospective study was to perform an epidemiological analysis on DME patients treated with intravitreal drugs in a tertiary hospital. The clinical outcome, adverse drug reactions (ADRs), and intravitreal drug expenses were assessed. Methods: All DME patients treated with Ranibizumab, Aflibercept, Dexamethasone implant, and Fluocinolone Acetonide implant at the Sassari University Hospital, Italy, between January 2017 and June 2020 were included. Central macular thickness (CMT) and best corrected visual acuity (BCVA) were measured. ADRs and drug expenses were analyzed. Results: Two-hundred thirty-one DME patients (mean age: 65 years) received intravitreal agents. Mean CMT and BCVA were 380 μm and 0.5 LogMAR at baseline, 298 μm and 0.44 logMAR after one year (p = 0.04), and 295 μm and 0.4 logMAR at the end of the follow-up period. A total of 1501 intravitreal injections were given; no major ADRs were reported. Treatment cost was €915,000 (€261,429/year). Twenty non-responders to Ranibizumab or Aflibercept were switched to a Dexamethasone implant. In these patients, mean CMT and BCVA were 468 µm and 0.5 LogMar at the time of switching and 362 µm and 0.3 LogMar at the end of the follow-up (p = 0.00014 and p = 0.08, respectively). Conclusion: Results confirm that Ranibizumab, Aflibercept, and Dexamethasone implant are effective and safe in DME treatment. A switch to Dexamethasone implant for patients receiving Aflibercept or Ranibizumab with minimal/no clinical benefit should be considered.
Assessment of Prescriptions in Elderly Patients Hospitalized in Medicine Departments
Authors: Giroux Audrey , Prudent Christelle , Jouanny Pierre , Muller Géraldine , Devilliers Hervé , Vadot Lucie
Published: November 2021

Drug-related iatrogenesis is an important issue in the elderly population, and preventing iatrogenic accidents helps to reduce hospitalizations. Our study’s objective was to evaluate prescriptions in the geriatric population of our establishment. The study conducted is a targeted clinical audit. Ten criteria were tested on the hospital prescriptions of people over 75 years old in 11 medical departments, before and after improvement actions. The non-compliance threshold was set at 10% of prescriptions for each criterion. In each phase, 165 patients were included. Four criteria were non-compliant (NC) in the first phase: the presence of Potentially Inappropriate Medications for the Elderly (PIMs) (NC = 57.6%), the adaptation of the medication to renal clearance (NC = 24.9%), the presence of illogical combination (NC = 9.7%), and the total anti-cholinergic score of the prescription (NC = 12.1%). After the implementation of improvement actions, the number of non-compliant criteria decreased between the two phases, from four to two. We obtained a significant improvement for three of the four criteria found to be non-compliant in the first phase. The criterion adaptation to renal function is close to compliance (NC = 10.1%) and the PIMs criterion remained non-compliant after reassessment (NC = 32.1%). Vigilance must be ongoing in order to limit drug iatrogeny, particularly in frail elderly patients.
Academic Expectations of Stress Inventory: A Psychometric Evaluation of Validity and Reliability of the Persian Version
Authors: Habibi Asgarabad Mojtaba , Charkhabi Morteza , Fadaei Zahra , Baker Julien S. , Dutheil Frederic
Published: November 2021

This study aims to evaluate the psychometric properties of the Academic Expectations of Stress Inventory (AESI) in terms of validity and reliability measurements among Persian students. A total sample of 620 high-school students (nfemale = 328, nmale = 292) was recruited to complete scales on academic expectations of stress, self-efficacy, and depression. The AESI was translated from English to Persian and its translation was further checked by three experts. We used a cross-sectional research design to collect data. The results approved the internal consistency, test–retest reliability, convergent, and construct validity of the ASEI. Additionally, confirmatory factor analysis confirmed the two-factor structure of the AESI, including the expectation of self and the expectations of parents/teachers. AESI was related to depression and self-efficacy in an empirically and theoretically expected direction. Moreover, configural and metric invariance were supported by gifted vs. non-gifted groups, but not scalar. No invariance was supported by gender groups. In conclusion, the psychometric properties of the Persian version of the AESI were confirmed to be used for educational, clinical, and research purposes in Iran.
Experimental Analysis of Mechanical Anisotropy of Selected Roofing Felts
Authors: Łuczak Bartosz , Sumelka Wojciech , Wypych Artur
Published: November 2021

In this work, four representatives of roofing felts are under consideration. Special attention is paid to the mechanical behaviour under the tensile load of the samples. The results of strength tests for the entire range of material work, from the first load to sample breaking, are shown with respect to a specific direction of sample cutting. Moreover, a unique study of the microstructure obtained with the scanning electron microscope and chemical composition determined by energy dispersive spectroscopy of the tested materials is presented. The significant mechanical material anisotropy is reported and moreover argued by microstructure characteristics. In perspective, the outcomes can give comprehensive knowledge on optimal usage of roofing felt and proper mathematical modelling.
Effects of Female Reproductive Hormone Levels on Inadvertent Intraoperative Hypothermia during Laparoscopic Gynecologic Surgery: A Retrospective Study
Authors: Lee Cheol , Park SeongNam , Kim ByoungRyun , Yim Hyeonbin , Lee Myeongjong , Lee Juhwan , Kim Hyungtae
Published: November 2021

Background and Objectives: Female reproductive hormones may affect core body temperature. This study aimed to investigate the effects of female reproductive hormones on inadvertent intraoperative hypothermia in patients who underwent laparoscopic gynecologic surgery under general anesthesia. Materials and Methods: This retrospective study included 660 menstruating and menopausal female patients aged 19–65 years. The patients were divided into two groups according to the occurrence of inadvertent intraoperative hypothermia: non-hypothermia group (N = 472) and hypothermia group (N = 188). After propensity score matching, 312 patients (N = 156 in each group) were analyzed to investigate the association between intraoperative hypothermia and female reproductive hormones. As potential predictors of inadvertent hypothermia, the levels of female reproductive hormones were analyzed using binary logistic regression. Results: The association of estradiol (r = −0.218, p = 0.000) and progesterone (r = −0.235, p = 0.000) levels with inadvertent intraoperative hypothermia was significant but weakly negative before matching; however, it was significant and moderately negative after matching (r = −0.326, p = 0.000 and r = −0.485, p = 0.000, respectively). In a binary logistic analysis, the odds ratio for estradiol was 0.995 (p = 0.014, 0.993 < 95% confidence interval [CI] < 0.998) before matching and 0.993 (p = 0.000, 0.862 < 95% CI < 0.930) after matching, and that for progesterone was 0.895 (p = 0.000, 0.862 < 95% CI < 0.930) before matching and 0.833 (p = 0.014, 0.990 < 95% CI < 0.996) after matching. Conclusions: Estradiol and progesterone levels were associated with inadvertent intraoperative hypothermia. However, the odds ratio for female reproductive hormone levels was close to 1. Therefore, female reproductive hormones may not be a risk factor for hypothermia during gynecologic surgery under general anesthesia. However, a small sample size in this study limits the generalizability of the results.
Maternal–Fetal Outcomes in Women with Endometriosis and Shared Pathogenic Mechanisms
Authors: Frincu Francesca , Carp-Veliscu Andreea , Petca Aida , Badiu Dumitru-Cristinel , Bratila Elvira , Cirstoiu Monica , Mehedintu Claudia
Published: November 2021

The connection between endometriosis and pregnancy outcomes is trending among the research topics. Until recently, endometriosis and its painful symptomatology were considered to be alleviated by pregnancy. However, these beliefs have shifted, as emerging literature has demonstrated the role of this condition in affecting pregnancy evolution. The underlying pathogenesis of endometriosis is still poorly understood, all the more when pregnancy complications are involved. Debatable opinions on endometriosis associated with obstetric complications exist because of the potential bias resulting from the heterogeneity of preceding evidence. This review aims to evaluate the connection between endometriosis and adverse pregnancy outcomes and their shared pathogenic mechanisms. We searched PubMed and EMBASE and focused on the studies that include placenta praevia, premature rupture of membranes, spontaneous preterm birth, gestational hypertension, preeclampsia, obstetric hemorrhages (ante- and postpartum bleeding, abruptio placentae), miscarriage, stillbirth, neonatal death, gestational diabetes mellitus, gestational cholestasis, small for gestational age, and their association with endometriosis. Not only the risks of emergence were highlighted, but also the pathogenic connections. Epigenetic alterations of some genes were found to be mirrored both in endometriosis and obstetric complications. This review issues a warning for providing increased attention to pregnant women with endometriosis and newborns as higher risks of preeclampsia, placental issues, and preterm deliveries are associated.
Dynamic Landscape of Extracellular Vesicle-Associated Proteins Is Related to Treatment Response of Patients with Metastatic Breast Cancer
Authors: Ruhen Olivia , Qu Xinyu , Jamaluddin M. Fairuz B. , Salomon Carlos , Gandhi Aesha , Millward Michael , Nixon Brett , Dun Matthew D. , Meehan Katie
Published: November 2021

Breast cancer is the leading cause of cancer death in women. The majority of these deaths are due to disease metastasis, in which cancer cells disseminate to multiple organs and disrupt vital physiological functions. It is widely accepted that breast cancer cells secrete extracellular vesicles (EVs), which contain dynamic molecular cargo that act as versatile mediators of intercellular communication. Therefore, Evs. secreted by breast cancer cells could be involved in the development of metastatic disease and resistance to treatment. Moreover, changes in EV cargo could reflect the effects of therapy on their parent tumor cells. The aim of this feasibility study was to quantitatively profile the proteomes of Evs. isolated from blood samples taken from treatment sensitive and resistant metastatic breast cancer patients to identify proteins associated with responses. Three serial blood samples were collected from three patients with metastatic breast cancer receiving systemic therapy including a responder, a non-responder, and a mixed-responder. Evs. were isolated from plasma using size exclusion chromatography and their protein cargo was prepared for tandem mass tag (TMT)-labelling and quantitative analyses using two-dimensional high-performance liquid chromatography followed by tandem mass spectrometry. After filtering, we quantitatively identified 286 proteins with high confidence using a q value of 0.05. Of these, 149 were classified as EV associated candidate proteins and 137 as classical, high abundant plasma proteins. After comparing EV protein abundance between the responder and non-responder, we identified 35 proteins with unique de-regulated abundance patterns that was conserved at multiple time points. We propose that this proof-of-concept approach can be used to identify proteins which have potential as predictors of metastatic breast cancer response to treatment.
Investigation of Potential Recovery Rates of Nickel, Manganese, Cobalt, and Particularly Lithium from NMC-Type Cathode Materials (LiNixMnyCozO2) by Carbo-Thermal Reduction in an Inductively Heated Carbon Bed Reactor
Authors: Windisch-Kern Stefan , Holzer Alexandra , Wiszniewski Lukas , Raupenstrauch Harald
Published: November 2021

Within the e-mobility sector, which represents a major driver of the development of the overall lithium-ion battery market, batteries with nickel-manganese-cobalt (NMC) cathode chemistries are currently gaining ground. This work is specifically dedicated to this NMC battery type and investigates achievable recovery rates of the valuable materials contained when applying an unconventional, pyrometallurgical reactor concept. For this purpose, the currently most prevalent NMC modifications (5-3-2, 6-2-2, and 8-1-1) with carbon addition were analyzed using thermogravimetric analysis and differential scanning calorimetry, and treated in a lab-scale application of the mentioned reactor principle. It was shown that the reactor concept achieves high recovery rates for nickel, cobalt, and manganese of well above 80%. For lithium, which is usually oxidized and slagged, the transfer coefficient into the slag phase was less than 10% in every experimental trial. Instead, it was possible to remove the vast amount of it via a gas phase, which could potentially open up new paths regarding metal recovery from spent lithium-ion batteries.
Surface Modification of a Nickel-Free Austenitic Stainless Steel by Low-Temperature Nitriding
Authors: Borgioli Francesca , Galvanetto Emanuele , Bacci Tiberio
Published: November 2021

Low-temperature nitriding allows to improve surface hardening of austenitic stainless steels, maintaining or even increasing their corrosion resistance. The treatment conditions to be used in order to avoid the precipitation of large amounts of nitrides are strictly related to alloy composition. When nickel is substituted by manganese as an austenite forming element, the production of nitride-free modified surface layers becomes a challenge, since manganese is a nitride forming element while nickel is not. In this study, the effects of nitriding conditions on the characteristics of the modified surface layers obtained on an austenitic stainless steel having a high manganese content and a negligible nickel one, a so-called nickel-free austenitic stainless steel, were investigated. Microstructure, phase composition, surface microhardness, and corrosion behavior in 5% NaCl were evaluated. The obtained results suggest that the precipitation of a large volume fraction of nitrides can be avoided using treatment temperatures lower than those usually employed for nickel-containing austenitic stainless steels. Nitriding at 360 and 380 °C for duration up to 5 h allows to produce modified surface layers, consisting mainly of the so-called expanded austenite or γN, which increase surface hardness in comparison with the untreated steel. Using selected conditions, corrosion resistance can also be significantly improved.
Fabrication of Antireflection Micro/Nanostructures on the Surface of Aluminum Alloy by Femtosecond Laser
Authors: Du Mengdan , Sun Quanquan , Jiao Wei , Shen Lifeng , Chen Xiao , Xiao Junfeng , Xu Jianfeng
Published: November 2021

Designed micro-nano structures on the surface of aluminum alloy provide excellent light trapping properties that can be used extensively in thermal photovoltaics, sensors, etc. However, the fabrication of high-performance antireflective micro-nano structures on aluminum alloy is challenging because aluminum has shallow intrinsic losses and weak absorption. A two-step strategy is proposed for fabricating broadband antireflection structures by superimposing nanostructures onto microscale structures. By optimizing the processing parameters of femtosecond laser, the average reflectances of 2.6% within the visible spectral region (400–800 nm) and 5.14% within the Vis-NIR spectral region (400–2500 nm) are obtained.
An Impedance Sensor for Distinguishing Multi-Contaminants in Hydraulic Oil of Offshore Machinery
Authors: Shi Haotian , Huo Dian , Zhang Hongpeng , Li Wei , Sun Yuqing , Li Guobin , Chen Haiquan
Published: November 2021

The cleanliness of hydraulic oil can reflect the service life of the oil and the wear state of hydraulic machinery. An impedance sensor is proposed to distinguish multi-contaminants in hydraulic oil. The impedance sensor has two detection modes: the inductance-resistance mode is used to detect metal debris, and the capacitance mode is used to distinguish water droplets and air bubbles. By adding a built-in silicon steel strip and an external silicon steel strip with high magnetic permeability, the distribution area, strength, and uniformity of the magnetic field are enhanced to improve the detection sensitivity under inductance and resistance parameters. In addition, the silicon steel strips are used as electrode plates to introduce capacitance parameter detection. The experimental results show that the resistance detection method based on coil successfully improves the detection ability for non-ferromagnetic metal debris. The impedance sensor for distinguishing multi-contaminants in hydraulic oil can provide technical support for fault diagnosis of offshore hydraulic machinery.
Exploring the Effect of a MnO2 Coating on the Electrochemical Performance of a Li1.2Mn0.54Ni0.13Co0.13O2 Cathode Material
Authors: Li Zhong , Yang Peiyue , Zheng Zhongxiang , Pan Qiyun , Liu Yisi , Li Yao , Xuan Jinnan
Published: November 2021

The effect of electrochemically active MnO2 as a coating material on the electrochemical properties of a Li1.2Mn0.54Ni0.13Co0.13O2 (LTMO) cathode material is explored in this article. The structural analysis indicated that the layered structure of the LTMO was unchanged after the modification with MnO2. The morphology inspection demonstrated that the rod-like LTMO particles were encapsulated by a compact coating layer. The MnO2 layer was able to hinder the electrolyte solution from corroding the LTMO particles and optimized the formation of a solid electrolyte interface (SEI). Meanwhile, lithium ions were reversibly inserted into and extracted from MnO2, which afforded an additional capacity. Compared with the bare LTMO, the MnO2-coated sample exhibited enhanced electrochemical performance. After the MnO2 coating, the first discharge capacity rose from 224.2 to 239.1 mAh/g, and the initial irreversible capacity loss declined from 78.2 to 46.0 mAh/g. Meanwhile, the cyclic retention climbed up to 88.2% after 100 cycles at 0.5 C, which was more competitive than that of the bare LTMO with a value of 71.1%. When discharging at a high current density of 2 C, the capacity increased from 100.5 to 136.9 mAh/g after the modification. These investigations may be conducive to the practical application of LTMO in prospective automotive Li-ion batteries.
A Cylindrical Molding Method for the Biofabrication of Plane-Shaped Skeletal Muscle Tissue
Authors: Nie Minghao , Shima Ai , Fukushima Kenta , Morimoto Yuya , Takeuchi Shoji
Published: November 2021

Muscle tissues can be fabricated in vitro by culturing myoblast-populated hydrogels. To counter the shrinkage of the myoblast-populated hydrogels during culture, a pair of anchors are generally utilized to fix the two ends of the hydrogel. Here, we propose an alternative method to counter the shrinkage of the hydrogel and fabricate plane-shaped skeletal muscle tissues. The method forms myoblast-populated hydrogel in a cylindrical cavity with a central pillar, which can prevent tissue shrinkage along the circumferential direction. By eliminating the usages of the anchor pairs, our proposed method can produce plane-shaped skeletal muscle tissues with uniform width and thickness. In experiments, we demonstrate the fabrication of plane-shaped (length: ca. 10 mm, width: 5~15 mm) skeletal muscle tissue with submillimeter thickness. The tissues have uniform shapes and are populated with differentiated muscle cells stained positive for myogenic differentiation markers (i.e., myosin heavy chains). In addition, we show the assembly of subcentimeter-order tissue blocks by stacking the plane-shaped skeletal muscle tissues. The proposed method can be further optimized and scaled up to produce cultured animal products such as cultured meat.
Enhancement of Flotation Performance of Oxidized Coal by the Mixture of Laurylamine Dipropylene Diamine and Kerosene
Authors: Zhang Qingshan , Niu Chenkai , Bu Xiangning , Bilal Muhammad , Ni Chao , Peng Yaoli
Published: November 2021

Conventional hydrocarbon oil cannot adhere effectively to oxidized coal, resulting in a low yield of clean coal. In this study, a high-speed homogenizer was used to emulsify LDD (laurylamine dipropylene diamine) and kerosene, which enhanced the flotation efficiency of oxidized coal. The flotation results showed an increase from 4.12% (only kerosene) to 23.33% (emulsified oil). An increase in contact angle indicated that the mixture reagent can increase the hydrophobicity of coal particles, which is attributed to the adsorption of LDD onto the coal particle surface and the decrease of the oil droplet A lower surface tension of LDD allows it to produce a stable layer of froth than the layer generated by kerosene alone.
Physically Switchable Antimicrobial Surfaces and Coatings: General Concept and Recent Achievements
Authors: Elashnikov Roman , Ulbrich Pavel , Vokatá Barbora , Pavlíčková Vladimíra Svobodová , Švorčík Václav , Lyutakov Oleksiy , Rimpelová Silvie
Published: November 2021

Bacterial environmental colonization and subsequent biofilm formation on surfaces represents a significant and alarming problem in various fields, ranging from contamination of medical devices up to safe food packaging. Therefore, the development of surfaces resistant to bacterial colonization is a challenging and actively solved task. In this field, the current promising direction is the design and creation of nanostructured smart surfaces with on-demand activated amicrobial protection. Various surface activation methods have been described recently. In this review article, we focused on the “physical” activation of nanostructured surfaces. In the first part of the review, we briefly describe the basic principles and common approaches of external stimulus application and surface activation, including the temperature-, light-, electric- or magnetic-field-based surface triggering, as well as mechanically induced surface antimicrobial protection. In the latter part, the recent achievements in the field of smart antimicrobial surfaces with physical activation are discussed, with special attention on multiresponsive or multifunctional physically activated coatings. In particular, we mainly discussed the multistimuli surface triggering, which ensures a better degree of surface properties control, as well as simultaneous utilization of several strategies for surface protection, based on a principally different mechanism of antimicrobial action. We also mentioned several recent trends, including the development of the to-detect and to-kill hybrid approach, which ensures the surface activation in a right place at a right time.
Narrow-Linewidth GaN-on-Si Laser Diode with Slot Gratings
Authors: Tang Yongjun , Feng Meixin , Liu Jianxun , Fan Shizhao , Sun Xiujian , Sun Qian , Zhang Shuming , Liu Tong , Kong Yaping , Huang Zengli , Ikeda Masao , Yang Hui
Published: November 2021

This letter reports room-temperature electrically pumped narrow-linewidth GaN-on-Si laser diodes. Unlike conventional distributed Bragg feedback laser diodes with hundreds of gratings, we employed only a few precisely defined slot gratings to narrow the linewidth and mitigate the negative effects of grating fabrication on the device performance. The slot gratings were incorporated into the ridge of conventional Fabry-Pérot cavity laser diodes. A subsequent wet etching in a tetramethyl ammonium hydroxide solution not only effectively removed the damages induced by the dry etching, but also converted the rough and tilted slot sidewalls into smooth and vertical ones. As a result, the threshold current was reduced by over 20%, and the reverse leakage current was decreased by over three orders of magnitude. Therefore, the room-temperature electrically pumped narrow-linewidth GaN-on-Si laser diode has been successfully demonstrated.
Laser-Induced Graphene Heater Pad for De-Icing
Authors: Lee Jun-Uk , Lee Chan-Woo , Cho Su-Chan , Shin Bo-Sung
Published: November 2021

The replacement of electro-thermal material in heaters with lighter and easy-to-process materials has been extensively studied. In this study, we demonstrate that laser-induced graphene (LIG) patterns could be a good candidate for the electro-thermal pad. We fabricated LIG heaters with various thermal patterns on the commercial polyimide films according to laser scanning speed using an ultraviolet pulsed laser. We adopted laser direct writing (LDW) to irradiate on the substrates with computer-aided 2D CAD circuit data under ambient conditions. Our highly conductive and flexible heater was investigated by scanning electron microscopy, transmission electron microscopy, Raman spectroscopy, thermogravimetric analysis, X-ray photoelectron spectroscopy, X-ray diffraction, and Brunauer–Emmett–Teller. The influence of laser scanning speed was evaluated for electrical properties, thermal performance, and durability. Our LIG heater showed promising characteristics such as high porosity, light weight, and small thickness. Furthermore, they demonstrated a rapid response time, reaching equilibrium in less than 3 s, and achieved temperatures up to 190 °C using relatively low DC voltages of approximately 10 V. Our LIG heater can be utilized for human wearable thermal pads and ice protection for industrial applications.
Inkjet Printing Infiltration of the Doped Ceria Interlayer in Commercial Anode-Supported SOFCs
Authors: Tomov Rumen I. , Mitchel-Williams Thomas B. , Venezia Eleonora , Kawalec Michal , Krauz Mariusz , Kumar Ramachandran Vasant , Glowacki Bartek A.
Published: November 2021

Single-step inkjet printing infiltration with doped ceria Ce0.9Ye0.1O1.95 (YDC) and cobalt oxide (CoxOy) precursor inks was performed in order to modify the properties of the doped ceria interlayer in commercial (50 × 50 × 0.5 mm3 size) anode-supported SOFCs. The penetration of the inks throughout the La0.8Sr0.2Co0.5Fe0.5O3−δ porous cathode to the Gd0.1Ce0.9O2 (GDC) interlayer was achieved by optimisation of the inks’ rheology jetting parameters. The low-temperature calcination (750 °C) resulted in densification of the Gd-doped ceria porous interlayer as well as decoration of the cathode scaffold with nanoparticles (~20–50 nm in size). The I–V testing in pure hydrogen showed a maximum power density gain of ~20% at 700 °C and ~97% at 800 °C for the infiltrated cells. The latter effect was largely assigned to the improvement in the interfacial Ohmic resistance due to the densification of the interlayer. The EIS study of the polarisation losses of the reference and infiltrated cells revealed a reduction in the activation polarisations losses at 700 °C due to the nano-decoration of the La0.8Sr0.2Co0.5Fe0.5O3−δ scaffold surface. Such was not the case at 800 °C, where the drop in Ohmic losses was dominant. This work demonstrated that single-step inkjet printing infiltration, a non-disruptive, low-cost technique, can produce significant and scalable performance enhancements in commercial anode-supported SOFCs.
Thin Film and Nanostructured Pd-Based Materials for Optical H2 Sensors: A Review
Authors: Sousanis Andreas , Biskos George
Published: November 2021

In this review paper, we provide an overview of state-of-the-art Pd-based materials for optical H2 sensors. The first part of the manuscript introduces the operating principles, providing background information on the thermodynamics and the primary mechanisms of optical detection. Optical H2 sensors using thin films (i.e., films without any nanostructuring) are discussed first, followed by those employing nanostructured materials based on aggregated or isolated nanoparticles (ANPs and INPs, respectively), as well as complex nanostructured (CN) architectures. The different material types are discussed on the basis of the properties they can attribute to the resulting sensors, including their limit of detection, sensitivity, and response time. Limitations induced by cracking and the hysteresis effect, which reduce the repeatability and reliability of the sensors, as well as by CO poisoning that deteriorates their performance in the long run, are also discussed together with an overview of manufacturing approaches (e.g., tailoring the composition and/or applying functionalizing coatings) for addressing these issues.
Organobeidellites for Removal of Anti-Inflammatory Drugs from Aqueous Solutions
Authors: Plevová Eva , Vallová Silvie , Vaculíková Lenka , Hundáková Marianna , Gabor Roman , Smutná Kateřina , Žebrák Radim
Published: November 2021

Diclofenac (DC) and ibuprofen (IBU) are widely prescribed non-steroidal anti-inflammatory drugs, the consumption of which has rapidly increased in recent years. The biodegradability of pharmaceuticals is negligible and their removal efficiency by wastewater treatment is very low. Therefore, the beidelitte (BEI) as unique nanomaterial was modified by the following different surfactants: cetylpyridinium (CP), benzalkonium (BA) and tetradecyltrimethylammonium (TD) bromides. Organobeidellites were tested as potential nanosorbents for analgesics. The organobeidellites were characterized using X-ray powder diffraction (XRD), Infrared spectroscopy (IR), Thermogravimetry and differential thermal analysis (TG/DTA) and scanning microscopy (SEM). The equilibrium concentrations of analgesics in solution were determined using UV-VIS spectroscopy. The intercalation of surfactants into BEI structure was confirmed both using XRD analysis due to an increase in basal spacing from 1.53 to 2.01 nm for BEI_BA and IR by decreasing in the intensities of bands related to the adsorbed water. SEM proved successful in the uploading of surfactants by a rougher and eroded organobeidellite surface. TG/DTA evaluated the decrease in dehydration/dehydroxylation temperatures due to higher hydrophobicity. The Sorption experiments demonstrated a sufficient sorption ability for IBU (55–86%) and an excellent ability for DC (over 90%). The maximum adsorption capacity was found for BEI_BA-DC (49.02 mg·g−1). The adsorption according to surfactant type follows the order BEI_BA > BEI_TD > BEI_CP.
Transcriptome-Wide Profile of 25-Hydroxyvitamin D3 in Primary Immune Cells from Human Peripheral Blood
Authors: Hanel Andrea , Bendik Igor , Carlberg Carsten
Published: November 2021

Vitamin D3 is an essential micronutrient mediating pleiotropic effects in multiple tissues and cell types via its metabolite 1α,25-dihydroxyvitamin D3 (1,25(OH)2D3), which activates the transcription factor vitamin D receptor. In this study, we used peripheral blood mononuclear cells (PBMCs) obtained from five healthy adults and investigated transcriptome-wide, whether the precursor of 1,25(OH)2D3, 25-hydroxyvitamin D3 (25(OH)D3), has gene regulatory potential on its own. Applying thresholds of >2 in fold change of gene expression and <0.05 as a false discovery rate, in this ex vivo approach the maximal physiological concentration of 25(OH)D3 (250 nM (nmol/L)) none of the study participants had a significant effect on their PBMC transcriptome. In contrast, 1000 and 10,000 nM 25(OH)D3 regulated 398 and 477 genes, respectively, which is comparable to the 625 genes responding to 10 nM 1,25(OH)2D3. The majority of these genes displayed specificity to the tested individuals, but not to the vitamin D metabolite. Interestingly, the genes MYLIP (myosin regulatory light chain interacting protein) and ABCG1 (ATP binding cassette subfamily G member 1) showed to be specific targets of 10,000 nM 25(OH)D3. In conclusion, 100- and 1000-fold higher 25(OH)D3 concentrations than the reference 10 nM 1,25(OH)2D3 are able to affect the transcriptome of PBMCs with a profile comparable to that of 1,25(OH)2D3.
Dysregulated Arginine Metabolism in Young Patients with Chronic Persistent Asthma and in Human Bronchial Epithelial Cells
Authors: Zhou Bo , Jiang Gulinigaer Tuerhong , Liu Hui , Guo Manyun , Liu Junhui , She Jianqing
Published: November 2021

Background: Recent metabolomics studies have found circulatory metabolism alterations in patients with asthma, indicating that altered metabolites played a significant role in asthma. However, the regulatory mechanisms in asthma, especially in young chronic persistent asthma remain underexplored. Methods: In this study, a prospective cohort of 162 patients diagnosed of asthma admitted to the First Affiliated Hospital of Xi’an Jiaotong University from January 2018 to December 2019 was used to perform a nested case-control study. Among them, we included 30 patients with chronic persistent asthma between 20 to 35 years old; 30 health control with evenly distributed age and sex were then recruited. Nontargeted metabolomics was applied to identify serum metabolic profiles and altered metabolic pathways. Results: In vitro, human bronchial epithelial cells (HBECs) line BEAS-2B with the addition of L-citrulline and/or asymmetric dimethylarginine (ADMA) model was utilized and the concentrations of nitric oxide (NO) metabolites were tested to evaluate the therapeutic potential of L-citrulline. The young patients with chronic persistent asthma displayed dysregulated serum metabolic profiles, especially enriched in arginine metabolism. The ratio of L-citrulline to ornithine is associated with blood eosinophil count. In vitro, adding L-citrulline could reverse ADMA-mediated reduction of NOx at lower L-arginine concentration (25 μM), but was ineffective in the higher L-arginine concentration (100 μM) media. Conclusions: The arginine metabolism balance is of vital importance during the pathogenesis and progression of chronic asthma. L-citrulline could be a powerful approach to restore airway NO production, potentially exhibiting therapeutic benefits among young patients with chronic asthma.
Role of Citrus Fruit Juices in Prevention of Kidney Stone Disease (KSD): A Narrative Review
Authors: Barghouthy Yazeed , Somani Bhaskar K.
Published: November 2021

To explore the relationship between citrus fruit juices (oranges, grapefruits, and lemonades) and kidney stone disease (KSD). Methods: A systematic review was performed using the Medline, EMBASE, and Scopus databases, in concordance with the PRISMA checklist for all English, French, and Spanish language studies regarding the consumption of citrus fruit juices and the relationship to urinary stone disease. The main outcome of interest was the association of citrus fruit juices with KSD. Results: Thirteen articles met the criteria for inclusion in the final review. Three large epidemiological studies found that grapefruit juice was a risk factor for stone formation, while orange juice did not increase the risk for KSD. Ten small prospective clinical studies found that orange, grapefruit, and lemon juices all increased urinary citrate levels. Only orange and grapefruit juices had an alkalinizing effect and while lemon juice has a protective effect by raising urinary citrate levels, it lacked a significant alkalinizing effect on urine pH. Orange juice and grapefruit juices significantly increased urinary oxalate levels, while orange juice also had a high carbohydrate content. Conclusion: While orange juice seems to play a protective role against stone formation, grapefruit was found to raise the risk of KSD in epidemiological studies but had a protective role in smaller clinical studies. Lemon juice had a smaller protective role than orange juice. Larger amounts of, as well as more accurate, data is needed before recommendations can be made and a high carbohydrate content in these juices needs to be taken into consideration.
Dietary Fibre Intake and the 10-Year Incidence of Tinnitus in Older Adults
Authors: Tang Diana , Tran Yvonne , Shekhawat Giriraj S. , Burlutsky George , Mitchell Paul , Gopinath Bamini
Published: November 2021

Tinnitus is the phantom perception of sound in the ears or head that increases in prevalence as age increases. With strong evidence supporting the benefits of dietary fibre for vascular health and hearing loss, intake of dietary fibre may also have a role in the prevention of tinnitus symptoms. This longitudinal study aims to determine the association between the intake of dietary fibre and other carbohydrate nutrition variables including glycaemic index (GI), glycaemic load (GL) and total carbohydrate intakes, and incident tinnitus over 10 years. Of the 1730 participants (aged ≥50 years) from the Blue Mountains Hearing Study with complete baseline data on tinnitus symptoms and carbohydrate intakes, 536 (31%) cases of tinnitus were identified and excluded from further incidence analysis. Dietary data were collected using a validated semi-quantitative food frequency questionnaire to determine intakes of total dietary fibre and fibre contributions from cereals, vegetables, and fruit. A purpose-built database based on Australian GI values was used to calculate mean GI. Lower versus higher intakes of fruit fibre (≤3.6 g/day vs. >3.6 g/day) and cereal fibre (≤4.2 g/day vs. >4.2 g/day) were significantly associated with a 65% (HR = 1.65; 95% CI: 1.15–2.36) and 54% (HR = 1.54; 95% CI: 1.07–2.22) increased risk of developing tinnitus over 10 years, respectively. Associations between intake of other carbohydrate nutrients and incident tinnitus were mostly non-significant. In summary, our study showed modest associations between intake of dietary fibre and incident tinnitus. The protective effects of fibre, particularly insoluble fibre, could underlie observed associations by reducing the risk of tinnitus via vascular risk factors such as cardiovascular disease. Further longitudinal studies evaluating different types and sources of fibre and tinnitus risk are needed to confirm our study findings.
Molecular Typing and Antimicrobial Susceptibility Profiles of Streptococcus uberis Isolated from Sheep Milk
Authors: Rosa Nives Maria , Duprè Ilaria , Azara Elisa , Longheu Carla Maria , Tola Sebastiana
Published: November 2021

Intramammary infections are a major problem for dairy sheep farms, and Streptococcus uberis is one of the main etiological agents of ovine mastitis. Surveys on antimicrobial resistance are still limited in sheep and characterization of isolates is important for acquiring information on resistance and for optimizing therapy. In this study, a sampling of 124 S. uberis isolates collected in Sardinia (Italy) from sheep milk was analyzed by multilocus-sequence typing (MLST) and pulsed field gel electrophoresis (PFGE) for genetic relatedness. All isolates were also subjected to antimicrobial susceptibility analysis by the disk diffusion test using a panel of 14 antimicrobials. Resistance genes were detected by PCR assays. MLST analysis revealed that the isolates were grouped into 86 sequence types (STs), of which 73 were new genotypes, indicating a highly diverse population of S. uberis. The most frequently detected lineage was the clonal complex (CC)143, although representing only 13.7% of all characterized isolates. A high level of heterogeneity was also observed among the SmaI PFGE profiles, with 121 unique patterns. Almost all (96.8%) isolates were resistant to at least one antimicrobial, while all exhibited phenotypic susceptibility to oxacillin, amoxicillin-clavulanic acid and ceftiofur. Of the antimicrobials tested, the highest resistance rate was found against streptomycin (93.5%), kanamycin (79.8%) and gentamicin (64.5%), followed by novobiocin (25%) and tetracycline-TE (19.3%). Seventy-four (59.7%) isolates were simultaneously resistant to all aminoglycosides tested. Seventeen isolates (13.7%) exhibited multidrug resistance. All aminoglycosides-resistant isolates were PCR negative for aad-6 and aphA-3′ genes. Among the TE-resistant isolates, the tetM gene was predominant, indicating that the resistance mechanism is mainly mediated by the protection of ribosomes and not through the efflux pump. Three isolates were resistant to erythromycin, and two of them harbored the ermB gene. This is the first study reporting a detailed characterization of the S. uberis strains circulating in Sardinian sheep. Further investigations will be needed to understand the relationships between S. uberis genotypes, mastitis severity, and intra-mammary infection dynamics in the flock, as well as to monitor the evolution of antimicrobial resistance.
Molecular and Clinical Aspects of Chronic Manifestations in Chagas Disease: A State-of-the-Art Review
Authors: Medina-Rincón Germán J. , Gallo-Bernal Sebastián , Jiménez Paula A. , Cruz-Saavedra Lissa , Ramírez Juan David , Rodríguez María Juliana , Medina-Mur Ramón , Díaz-Nassif Gustavo , Valderrama-Achury María Daniela , Medina Héctor M.
Published: November 2021

Chronic manifestations of Chagas disease present as disabling and life-threatening conditions affecting mainly the cardiovascular and gastrointestinal systems. Although meaningful research has outlined the different molecular mechanisms underlying Trypanosoma cruzi’s infection and the host-parasite interactions that follow, prompt diagnosis and treatment remain a challenge, particularly in developing countries and also in those where the disease is considered non-endemic. This review intends to present an up-to-date review of the parasite’s life cycle, genetic diversity, virulence factors, and infective mechanisms, as well as the epidemiology, clinical presentation, diagnosis, and treatment options of the main chronic complications of Chagas disease.