bluuebunny/crossref_metadata_2025 huggingface dataset:
Transient Receptor Potential Cation Channel Subfamily V Member 4 Mediates Pyroptosis in Chronic Obstructive Pulmonary Disease
Authors: Rao Yafei , Gai Xiaoyan , Xiong Jing , Le Yanqing , Sun Yongchang
Published: December 2021

TRPV4, a calcium permeable cation selective channel, was found to be involved in chronic obstructive pulmonary disease (COPD) through releasing ATP and IL-1β. Pyroptosis, a newly discovered pro-inflammatory cell death, was induced by cigarette smoke (CS) in airway epithelial cells (AECs). More recent studies indicated that blocking Ca2+ influx effectively inhibited pyroptosis. Therefore, we asked whether TRPV4 mediated CS-induced pyroptosis of AECs and hence participated in the pathogenesis of COPD. We found that pyroptosis and TRPV4 were upregulated in AECs from patients with COPD and long-term CS-exposed mice. Moreover, pharmacological inhibition or knockdown of TRPV4 function alleviated CS extract (CSE)-induced pyroptosis by inhibiting NACHT, LRP, PYD domains-containing protein 3 (NLRP3) inflammasome/activated caspase-1/gasdermin D pathway, decreasing the number of PI positive cells and lactate dehydrogenase (LDH) release, decreasing the expression of pro- inflammatory interleukin gene (IL)-1β, IL-8, and IL-18 expression, as well as increasing anti-inflammatory gene expression [NAD(P)H quinone dehydrogenase 1 (NQO1), superoxide dismutase 2 (mitochondrial) (MNSOD), and catalase, (CAT)]. Moreover, pharmacological inhibition or knockdown of TRPV4 function significantly relieved CSE-induced mitochondrial damage including decreased mitochondrial membrane potential, mitochondrial fusion protein (OPA1, MFN2) expression, and increased mitochondrial fission protein (DRP1, MFF) expression. Taken together, these findings indicate that TRPV4 mediates AEC pyroptosis via NLRP3/caspase-1/GSDMD pathway in COPD.
Discovery of Single Nucleotide Polymorphisms for Resistance to Abnormal Vertical Growth in Macadamia
Authors: Zakeel Mohamed Cassim Mohamed , Alam Mobashwer , Geering Andrew D. W. , Topp Bruce , Akinsanmi Olufemi A.
Published: December 2021

Abnormal vertical growth (AVG) syndrome is a serious threat to the Australian macadamia industry as it decreases the yield of nuts by as much as 70% per annum. A lack of information on the cause of AVG has hindered the development of an effective disease management strategy. Discovery of genetic markers associated with disease resistance can be used as tool for rapid selection of elite cultivars, hence helps in efficient disease management. Differences in field susceptibility of macadamia cultivars provide an opportunity for discovery of genetic markers that are associated with host resistance. REML mixed model analysis was performed to estimate the AVG rating of 51 cultivars from multiple origins using phenotypic data from 359 trees planted in four sites. Most of the Hawaiian cultivars were found as susceptible, while selections from the Australian macadamia industry breeding program were predominantly resistant. All the cultivars were genotyped for 13,221 DArTseq-based single nucleotide polymorphism (SNP) markers. A bulked sample analysis was performed using 20 genotypes each at the extremes of AVG phenotypic ratings. Ten SNP markers were predicted to be associated with AVG resistance and two arbitrarily selected SNP markers were validated using PCR and Sanger sequencing. Our findings suggest that AVG resistance in the commercial cultivars may be derived from the genomic introgression of Macadamia tetraphylla through interspecific hybridization. The results may support marker-assisted selection for macadamia germplasm with AVG resistance.
Isoprenoid-Derived Metabolites and Sugars in the Regulation of Flowering Time: Does Day Length Matter?
Authors: Gawarecka Katarzyna , Ahn Ji Hoon
Published: December 2021

In plants, a diverse set of pathways regulate the transition to flowering, leading to remarkable developmental flexibility. Although the importance of photoperiod in the regulation of flowering time is well known, increasing evidence suggests the existence of crosstalk among the flowering pathways regulated by photoperiod and metabolic pathways. For example, isoprenoid-derived phytohormones (abscisic acid, gibberellins, brassinosteroids, and cytokinins) play important roles in regulating flowering time. Moreover, emerging evidence reveals that other metabolites, such as chlorophylls and carotenoids, as well as sugar metabolism and sugar accumulation, also affect flowering time. In this review, we summarize recent findings on the roles of isoprenoid-derived metabolites and sugars in the regulation of flowering time and how day length affects these factors.
Genetic and Molecular Mechanisms Conferring Heat Stress Tolerance in Tomato Plants
Authors: Hoshikawa Ken , Pham Dung , Ezura Hiroshi , Schafleitner Roland , Nakashima Kazuo
Published: December 2021

Climate change is a major threat to global food security. Changes in climate can directly impact food systems by reducing the production and genetic diversity of crops and their wild relatives, thereby restricting future options for breeding improved varieties and reducing the ability to adapt crops to future challenges. The global surface temperature is predicted to rise by an average of 0.3°C during the next decade, and the Paris Agreement (Paris Climate Accords) aims to limit global warming to below an average of 2°C, preferably to 1.5°C compared to pre-industrial levels. Even if the goal of the Paris Agreement can be met, the predicted rise in temperatures will increase the likelihood of extreme weather events, including heatwaves, making heat stress (HS) a major global abiotic stress factor for many crops. HS can have adverse effects on plant morphology, physiology, and biochemistry during all stages of vegetative and reproductive development. In fruiting vegetables, even moderate HS reduces fruit set and yields, and high temperatures may result in poor fruit quality. In this review, we emphasize the effects of abiotic stress, especially at high temperatures, on crop plants, such as tomatoes, touching upon key processes determining plant growth and yield. Specifically, we investigated the molecular mechanisms involved in HS tolerance and the challenges of developing heat-tolerant tomato varieties. Finally, we discuss a strategy for effectively improving the heat tolerance of vegetable crops.
AnDHN, a Dehydrin Protein From Ammopiptanthus nanus, Mitigates the Negative Effects of Drought Stress in Plants
Authors: Sun Yibo , Liu Linghao , Sun Shaokun , Han Wangzhen , Irfan Muhammad , Zhang Xiaojia , Zhang Li , Chen Lijing
Published: December 2021

Dehydrins (DHNs) play crucial roles in a broad spectrum of abiotic stresses in model plants. However, the evolutionary role of DHNs has not been explored, and the function of DHN proteins is largely unknown in Ammopiptanthus nanus (A. nanus), an ancient and endangered legume species from the deserts of northwestern China. In this study, we isolated a drought-response gene (c195333_g1_i1) from a drought-induced RNA-seq library of A. nanus. Evolutionary bioinformatics showed that c195333_g1_i1 is an ortholog of Arabidopsis DHN, and we renamed it AnDHN. Moreover, DHN proteins may define a class of proteins that are evolutionarily conserved in all angiosperms that have experienced a contraction during the evolution of legumes. Arabidopsis plants overexpressing AnDHN exhibited morpho-physiological changes, such as an increased germination rate, higher relative water content (RWC), higher proline (PRO) content, increased peroxidase (POD) and catalase (CAT) activities, lower contents of malondialdehyde (MDA), H2O2 and O2–, and longer root length. Our results showed that the transgenic lines had improved drought resistance with deep root system architecture, excellent water retention, increased osmotic adjustment, and enhanced reactive oxygen species (ROS) scavenging. Furthermore, the transgenic lines also had enhanced salt and cold tolerance. Our findings demonstrate that AnDHN may be a good candidate gene for improving abiotic stress tolerance in crops.

Key Message: Using transcriptome analysis in Ammopiptanthus nanus, we isolated a drought-responsive gene, AnDHN, that plays a key role in enhancing abiotic stress tolerance in plants, with strong functional diversification in legumes.
Country Indicators Moderating the Relationship Between Phubbing and Psychological Distress: A Study in 20 Countries
Authors: Błachnio Agata , Przepiórka Aneta , Gorbaniuk Oleg , McNeill Monika , Bendayan Rebecca , Durak Mithat , Senol-Durak Emre , Ben-Ezra Menachem , Benvenuti Martina , Angeluci Alan , Abreu Ana Maria , Makita Meiko , Blanca María J. , Brkljacic Tihana , Babič Nenad Č. , Gorbaniuk Julia , Holdoš Juraj , Ivanova Ana , Malik Sadia , Milanovic Anita , Musil Bojan , Pantic Igor , Rando Belén , Seidman Gwendolyn , D’Souza Lancy , Vanden Abeele Mariek M. P. , Wołońciej Mariusz , Wu Anise M. S. , Yu Shu , Mazzoni Elvis
Published: December 2021

Problematic mobile phone use can be related to negative mental states. Some studies indicate that behavioural dependency is related to variables associated with the country of origin. The aim of our study was to investigate if country indicators moderated the relationship between phubbing and psychological distress. Our sample consisted of 7,315 individuals from 20 countries, who completed the Phubbing Scale and the Kessler Psychological Distress Scale (K6). The analyses also included country indicators: the Gender Gap Index (GGI), the Human Development Index (HDI), the Social Progress Index (SPI), Hofstede’s dimensions of culture, and the World Happiness Index (WHI). Our results showed that psychological distress was related to at least one dimension of phubbing (i.e., to communication disturbance or phone obsession) in all countries, which means this relationship is culturally universal. The results of the study demonstrate the importance of testing measurement invariance to determine what type of analysis and what type of conclusion are valid in a given study or comparison. Moreover, the increasing or decreasing correlation between phubbing and distress is related to some culture-level indices.
Perceived Coach-Created Motivational Climates as Predictors of Athletes’ Goal Reengagement: The Mediational Role of Goal Motives
Authors: Martínez-González Natalia , Atienza Francisco L. , Tomás Inés , Balaguer Isabel
Published: December 2021

Athletes have to face several challenges during the sport season, and one of them could involve dealing with unattainable goals. In these situations, being able to reengage in other goals as a form of goal adjustment and in response to contextual demands is adaptive. According to previous literature, some aspects of the athletes’ social context, such as coach-created motivational climates, could encourage more adaptive responses in athletes, and so it is possible that these climates would also promote athletes’ goal regulation and goal reengagement. The purpose of this study was twofold: to analyze whether athletes’ perception of empowering and disempowering climates were related to their goal reengagement through the mediation of goal motives; and to examine the interaction between the two climates when they predict reengagement through athletes’ goal motives. Participants were 414 Spanish university athletes (49.5% male, 50.5% female) who belonged to different university teams, with ages ranging from 17 to 33 years old (M= 20.61,SD= 2.58). In the sport facilities, all of them completed questionnaires that evaluated their perception of empowering and disempowering climates, their goal motives, and their goal reengagement. Structural equation modeling (SEM) results showed that perceived empowering climate positively predicted autonomous goal motives, which in turn had a positive relationship with goal reengagement. Conversely, perceived disempowering climate positively predicted controlled goal motives, which were not related to goal reengagement. Thus, we only found support for the indirect relationship between perceived empowering climate and goal reengagement through autonomous goal motives. Moderated mediation analyses revealed that interaction effects between perceived empowering and disempowering climates were not significant in the prediction of goal reengagement through goal motives. Findings revealed that the perception of empowering climates promotes athletes’ goal reengagement when goals become unattainable via the increase in their autonomous goal motives. Conversely, when athletes perceive disempowering climates, they have more controlled goal motives, which are not related to goal reengagement. In addition, the study supports the need to educate coaches to create more empowering and less disempowering climates.
Modern Physical Education and Its Influence on Students’ Entrepreneurial Psychology in Sports Universities
Authors: Zhou Daliang , Zhu Delong , Zhang Fengrui , Li Guangxue , Zong Ke
Published: December 2021

This study aims to explore the entrepreneurial psychology of physical Education (PE) students under the “Internet+”environment, to cultivate and improve the entrepreneurial consciousness of PE students, taking the realization of students’ sense of self-efficacy as an intermediary factor. The new educational technology in modern PE is analyzed first. Specifically, the motion sensing technology based on human-computer natural interaction can be used for training, so that learners can effectively improve their physical skills. Subsequently, the current entrepreneurial situation of PE majors is discussed, with 188 students from Tianjin University of Sport and Guangzhou Sport University selected as research subjects. It is found that 62.2% of students have never been exposed to online entrepreneurship, and they are more afraid of entrepreneurial risks. In terms of entrepreneurial motivation, most students choose to start a business because of “personal ideals,” and only 40 people choose to start a business because of economic factors. There is a significant positive correlation between entrepreneurial self-efficacy and entrepreneurial intention of college students majoring in PE, and the correlation coefficient is 0.488. At present, the teaching mode of sports universities focuses on the teaching of professional courses. However, students generally believe that the professional knowledge learned is not useful for future entrepreneurship. The entrepreneurial self-efficacy of college students tends to be positive, and there are notable differences in the entrepreneurial self-efficacy between boys and girls. The regression analysis of entrepreneurial self-efficacy and entrepreneurial intention of college students shows that entrepreneurial self-efficacy can effectively predict entrepreneurial intention. This research promotes the innovation and development of the sports industry under the background of “Internet+”.
Improving Health of People With Multiple Sclerosis From a Multicenter Randomized Controlled Study in Parallel Groups: Preliminary Results on the Efficacy of a Mindfulness Intervention and Intention Implementation Associated With a Physical Activity Program
Authors: Torkhani Eya , Dematte Emilie , Slawinski Jean , Csillik Antonia , Gay Marie-Claire , Bensmaïl Djamel , Heinzlef Olivier , de Marco Giovanni
Published: December 2021

Objectives: The objective of this study is to investigate the efficacy of psychological Interventions – Mindfulness or Implementation Intention – associated with a Physical Activity program, delivered via internet, in reducing Multiple Sclerosis symptoms.

Method: Thirty-five adults were randomly assigned to one of the three groups: a Mindfulness-Based Intervention group (N = 12), Implementation Intention group (N = 11), and a Control Group (N = 12). All the groups received the same Physical Activity program. The Mindfulness condition group received daily training in the form of pre-recorded sessions while the Implementation group elaborated their specific plans once a week. Mobility, fatigue, and the impact of the disease on the patient’s life were measured. Two measurement times are carried out in pre-post intervention, at baseline and after eight weeks.

Results: Overall, after 8 weeks intervention, results show that there was a significant increase in Walking distance in the three groups. In addition, the within-group analysis showed a statistically significant improvement between pre and post intervention on the physical component of the Disease Impact scale in the Implementation Intention group (p = 0.023) with large effect size, in the Mindfulness-Based Intervention group (p = 0.008) with a medium effect size and in the control group (p = 0.028) with small effect size. In the Implementation Intention group, all physical, psychosocial and cognitive Fatigue Impact subscales scores decreased significantly (p = 0.022, p = 0.023, and p = 0.012, respectively) and the physical component was statistically and negatively correlated (r = −0.745; p = 0.008) when Implementation Intention group practice a mild to moderate physical activity. In the Mindfulness-Based Intervention group, the physical component (MFIS) showed a statistically significant improvement (p = 0.028) but no correlation with moderate-to-vigorous physical activity (MVPA); the control group outcomes did not reveal any significant change.

Conclusion: The results of this study are very encouraging and show the feasibility of Mindfulness interventions associated with physical activity to improve the health of people with MS. Further study should assess Mindfulness interventions tailored to MS condition and using both hedonic and eudemonic measures of happiness.
Phrase Depicting Immoral Behavior Dilates Its Subjective Time Judgment
Authors: Jia Lina , Shao Bingjie , Wang Xiaocheng , Shi Zhuanghua
Published: December 2021

Intuitive moral emotions play a major role in forming our opinions and moral decisions. However, it is not yet known how we perceive the subjective time of moral-related information. In this study, we compared subjective durations of phrases depicting immoral, disgust, or neutral behaviors in a duration bisection task and found that phrases depicting immoral behavior were perceived as lasting longer than the neutral and disgusting phrases. By contrast, the subjective duration of the disgusting phrase, unlike the immoral phrase, was comparable to the neutral phrase. Moreover, the lengthening effect of the immoral phrase relative to the neutral phrase was significantly correlated to the anonymously prosocial tendency of the observer. Our findings suggest that immoral phrases induce embodied moral reaction, which alters emotional state and subsequently lengthens subjective time.
Twitter Mediated Sociopolitical Communication During the COVID-19 Pandemic Crisis in India
Authors: Jain Nishtha , Malviya Preet , Singh Purnima , Mukherjee Sumitava
Published: December 2021

While Twitter has grown popular among political leaders as a means of computer-mediated mass media communication alternative, the COVID-19 pandemic required new strategies for socio-political communication to handle such a crisis. Using the case of India, which was one of the worst-hit countries and is also the world’s largest democracy, this research explicates how political leaders responded to the COVID-19 crisis on Twitter during the first wave as it was the first time such a crisis occurred. Theoretical frameworks of discursive leadership and situational crisis communication theory have been used to analyze interactions based on the usage patterns, the content of communication, the extent of usage in relation to the severity of the crisis, and the possible role of leaders’ position along with the status of their political party. The sample consisted of tweets posted by six prominent political leaders in India across the four consecutive lockdown periods from 25th March to 31st May 2020. A total of 4,158 tweets were scrapped and after filtering for retweets, the final dataset consisted of 2,809 original tweets. Exploratory data analysis, sentiment analysis, and content analysis were conducted. It was found that the tweets had an overall positive sentiment, an important crisis management strategy. Four main themes emerged: crisis management information, strengthening followers’ resilience and trust, reputation management, and leaders’ proactiveness. By focusing on such discursive aspects of crisis management, the study comprehensively highlights how political interactions on twitter integrated with politics and governance to handle COVID-19 in India. The study has implications for the fields of digital media interaction, political communication, public relations, and crisis leadership.
Educational Neuroscience Training for Teachers’ Technological Pedagogical Content Knowledge Construction
Authors: Cui Yulu , Zhang Hai
Published: December 2021

The attention to the laws of the brain and the mechanism of learning in the smart education era becomes the starting point for the convergence and development of education and neuroscience, which also inspired educational neuroscience (EN) affecting the teacher’s development. Although teachers always have a general curiosity about EN and its applications, the limited knowledge hinders their general practice, neuromyths begin to emerge, and there is no evidence to directly show the connection between EN and teachers’ technological pedagogical content knowledge (TPACK) knowledge. Based on an EN teacher training program for 216 teachers, this study verifies that EN training programs can promote teachers’ understanding of EN-related knowledge, and EN is also correlated to teachers’ TPACK. However, the EN training program does not promote high well-being and satisfaction. The research also analyzes the process of teachers’ EN knowledge dissemination based on interviews, and the research conclusion can further reveal the necessity of EN training for teachers in the future.
Multi-Family Therapy for First Episode Psychosis: Experiences of Families in Singapore
Authors: Loh Christopher , Liang Wilfred , Lee Helen , Koh Astelle
Published: December 2021

Aim: This qualitative study examined the experiences of families with Multi-Family Therapy (MFT) provided by the Early Psychosis Intervention Programme (EPIP) in Singapore. The MFT was piloted over a period of 2 years and findings from this study were used to further refine the MFT to better meet the needs of Singaporean families in the service.

Methods: Families who completed the MFT were invited to participate in the study. Nine clients and ten carers who consented to participate in the study were allocated to two client and two carer Focus Group Discussions (FGDs) respectively. A semi-structured interview schedule was used to facilitate the discussions. The FGDs were audio recorded, transcribed, and anonymised. The data was analysed using thematic analysis.

Results: Four main themes emerged from the analysis: (1) therapeutic processes of MFT, (2) positive changes in family relationships, (3) improvements in coping with psychosis, and (4) suggestions for improvement in MFT. The families suggested some structural changes to the MFT, and more carers than clients would prefer therapists to offer more expert advice.

Conclusions: Findings suggest that a Western-based MFT can be adapted to work with Singaporean families. This study sheds light on the therapeutic processes that may be related to the changes in family relationships and coping with psychosis. In addition, it suggests that therapists taking an expert and authoritative approach may not fit with the needs of younger generations in Singapore. It advocates for therapists to take a flexible and fluid stance to work with Singaporean families.
Association of Negative Symptoms of Schizophrenia Assessed by the BNSS and SNS Scales With Neuropsychological Performance: A Gender Effect
Authors: Wójciak Paweł , Domowicz Klaudia , Zabłocka Marta , Michalak Michał , Rybakowski Janusz K.
Published: December 2021

Objective: The relationship between negative symptoms and neurocognitive performance in schizophrenia is well documented, but the mechanism of these connections remains unclear. The study aims to measure the relationship between the results on the new scales for the assessment of negative symptoms such as Brief Negative Symptom Scale (BNSS) and Self-evaluation of Negative Symptoms (SNS), and the results of some neurocognition tests. The second aim is to assess a possible gender effect on these associations.

Methods: The study included 80 patients (40 men, 40 women) with schizophrenia, aged 19–63 (mean 38 years), during the improvement period (total PANSS score <80, unchanged pharmacological treatment in the last 3 weeks). They were assessed using the BNSS, SNS, Personal and Social Performance (PSP) scales, and the tests for neuropsychological performance such as the Trail Making Test (TMT-A, TMT-B), Stroop Color-Word Interference Test, Verbal fluency tests (VFT), Category fluency test (CFT), and Digit Symbol Substitution Test (DSST).

Results: Male patients obtained higher scores than females on some PANSS and BNSS items. No gender differences were observed for the SNS scale. Female patients scored better in the PSP and CFT. In male patients, a significant positive correlation between the intensity of negative symptoms measured by the BNSS and the results of PSP with the Trail Making Test was observed. In female patients, we found a positive correlation between the results of BNSS and PSP with the Stroop Color-Word Interference Test.

Conclusion: The obtained results confirm the relationship between negative symptoms and neurocognition in schizophrenia patients. However, in male and female patients such association was observed for different cognitive domains. Further research is needed to explain the nature of these differences.
The Persian Language Version of the Obsession With the COVID-19 Scale for Adolescents
Authors: Asanjarani Faramarz , Szczygieł Monika , Arif Amna
Published: December 2021

Objective: The worldwide pandemic of coronavirus disease (COVID-19) has caused significant public health burdens and psychological dysfunctions. In this challenging time, adolescents require special care. The Persian version of the Obsession with COVID-19 Scale (OCS) for adolescents was developed to screen for dysfunctional obsession associated with the coronavirus during the global pandemic. The structure and internal consistency of the OCS were established.

Design and Measures: Although there are different language versions of the OCS, this is the first study to validate the psychometric properties of the OCS in Iranian adolescents. Seven hundred and nine students (369 girls) participated in the study. Demographic questions and the OCS were administered.

Results: The findings provided support for the existence of a unidimensional structure that met the criteria for configural, metric, and full scalar invariance across gender (girls and boys), inhabitancy (urban and rural), and infection experience (infected and non-infected). The OCS is short and highly reliable measurement. However, further research is necessary to establish the validity of the scale in Iranian population.

Conclusions: The development of such valid scales is an essential part of both research and practice during times of crisis, like a global pandemic. Diagnosis of pandemic related to obsessive thoughts in adolescents is needed as the COVID-19 pandemic is still ongoing and as experts point out, it can be expected that the effects of the pandemic will be observed in the coming years. The Persian version makes it possible to conduct international comparative research on the anxiety related to the COVID-19 pandemic.
Effects of Mindfulness-Based Cognitive Therapy on Peripheral Markers of Stress and Inflammation in Older-Adults With Depression and Anxiety: A Parallel Analysis of a Randomized Controlled Trial
Authors: Belliveau Claudia , Nagy Corina , Escobar Sophia , Mechawar Naguib , Turecki Gustavo , Rej Soham , Torres-Platas Susana G.
Published: December 2021

Background: Depression and anxiety are prevalent in older-adults and often difficult to treat: up to 55% of patients are unresponsive to pharmacotherapy. Mindfulness-Based Cognitive Therapy (MBCT) is a promising treatment, however, its biological mechanisms remain unknown in older-adults.

Methods: We examined if, in older-adults, decreased depression and anxiety symptoms after MBCT are associated with changes in the expression levels of C-reactive protein, Interleukin-1β, Monocyte chemoattractant protein-1 and mineralocorticoid receptor compared to treatment as usual (TAU). Older-adults (age ≥60) with depression and anxiety were randomized to MBCT or treatment as usual. Gene expression levels from blood samples were measured using quantitative polymerase chain reaction (n = 37) at baseline and after 8-weeks of MBCT or TAU.

Results: As previously published, we found a significant reduction in symptoms of depression F (1, 35) = 10.68, p = 0.002, partial η2 = 0.23 and anxiety F (1, 35) = 9.36, p = 0.004, partial η2 = 0.21 in geriatric participants following MBCT compared to TAU. However, the expression levels of measured genes were not significantly different between groups and were not associated with changes in depression and anxiety symptoms.

Conclusion: Our results suggest that the symptom reduction following MBCT in older-adults may not be accompanied by changes in the stress-response and inflammatory pathways. Future research should address other potential biological alterations associated to MBCT that may be responsible for the reduction of symptoms.
Analysis of Clinical Characteristics and Virus Strains Variation of Patients Infected With SARS-CoV-2 in Jiangsu Province—A Retrospective Study
Authors: Wang Shenjiao , Zou Xin , Li Zhifeng , Fu Jianguang , Fan Huan , Yu Huiyan , Deng Fei , Huang Haodi , Peng Jiefu , Zhao Kangcheng , Cui Lunbiao , Zhu LiGuo , Bao Changjun
Published: December 2021

Background: At present, the global sever acute respiratory syndrome coronavirus 2 (SARS-CoV-2) situation is still grim, and the risk of local outbreaks caused by imported viruses is high. Therefore, it is necessary to monitor the genomic variation and genetic evolution characteristics of SARS-CoV-2. The main purpose of this study was to detect the entry of different SARS-CoV-2 variants into Jiangsu Province, China.

Methods: First, oropharyngeal swabs were collected from 165 patients (55 locally confirmed cases and 110 imported cases with confirmed and asymptomatic infection) diagnosed with SARS-CoV-2 infection in Jiangsu Province, China between January 2020 and June 2021. Then, whole genome sequencing was used to explore the phylogeny and find potential mutations in genes of the SARS-CoV-2. Last, association analysis among clinical characteristics and SARS-CoV-2 Variant of Concern, pedigree surveillance analysis of SARS-COV-2, and single nucleotide polymorphisms (SNPs) detection in SARS-COV-2 samples was performed.

Results: More men were infected with the SARS-CoV-2 when compared with women. The onset of the SARS-CoV-2 showed a trend of younger age. Moreover, the number of asymptomatic infected patients was large, similar to the number of common patients. Patients infected with Alpha (50%) and Beta (90%) variants were predominantly asymptomatic, while patients infected with Delta (17%) variant presented severe clinical features. A total of 935 SNPs were detected in 165 SARS-COV-2 samples. Among which, missense mutation (58%) was the dominant mutation type. About 56% of SNPs changes occurred in the open reading frame 1ab (ORF1ab) gene. Approximately, 20% of SNP changes occurred in spike glycoprotein (S) gene, such as p.Asp501Tyr, p.Pro681His, and p.Pro681Arg. In total, nine SNPs loci in S gene were significantly correlated with the severity of patients. It is worth mentioning that amino acid substitution of p.Asp614Gly was significantly positively correlated with the clinical severity of patients. The amino acid replacements of p.Ser316Thr and p.Lu484Lys were significantly negatively correlated with the course of disease.

Conclusion: Sever acute respiratory syndrome coronavirus 2 (SARS-CoV-2) may further undergo a variety of mutations in different hosts, countries, and weather conditions. Detecting the entry of different virus variants of SARS-CoV-2 into Jiangsu Province, China may help to monitor the spread of infection and the diversity of eventual recombination or genomic mutations.
Patterns of Self-Care Behaviors and Their Influence on Maintaining Independence: The National Health and Aging Trends Study
Authors: Mielenz Thelma J. , Kannoth Sneha , Xue Qian-Li
Published: December 2021

Importance: Few studies have addressed the combined effects of health-promoting and self-care behaviors among older adults. Thus, new research is needed to assess the potential for behavior change to prolong independence in later life.

Objectives: To determine the relationships between self-care behaviors and risks of mobility and activities of daily living (ADLs) over time.

Design: Longitudinal data was used from the National Health and Aging Trends Study (NHATS) cohort. Eight baseline self-care behaviors were summarized using latent class analysis. Separately, longitudinal latent classes of mobility and ADLs were created.

Setting: Annual in-person interviews conducted for a nationally representative sample.

Participants: The baseline study sample included 7,609 Medicare beneficiaries aged ≥65 from NHATS who were living in community or residential care settings, with a 71% response rate. The average age was 75, with 57% female, 81% white and 78% high school graduates or higher. Approximately, 80% (n = 6,064) completed 5 years of follow-up.

Exposures: Favorable vs. unfavorable self-care latent classes measured at baseline.

Main outcomes and Measures: Associations were measured between baseline classes and longitudinal classes of mobility and ADLs difficulty. Among decedents, 5-year associations were measured between baseline classes and years of overall, healthy, able, and healthy/able life.

Results: Two habitual baseline self-care behavioral patterns (46% favorable; 54% unfavorable) and three trajectories of change in mobility and ADLs disability (maintaining independence; shifting to accommodation/difficulty; shifting to assistance) emerged over time. Participants with a favorable baseline pattern had 92% (0.90–0.94) reduced risk in shifting to assistance class and 70% (0.64–0.76) reduced risk for shifting to accommodation/difficulty class for mobility disability. Participants with a favorable baseline pattern had 86% (0.83–0.89) reduced risk in shifting to assistance class and 24% (0.11–0.36) reduced risk in shifting to accommodation/difficulty class for ADLs disability. Those with an unfavorable pattern had 2.54 times greater risk of mortality by the end of the 5-year follow-up compared to those with a favorable pattern.

Conclusion: Self-care behaviors in older age represent a habitual pattern. A favorable self-care behavioral pattern decreased the risk of moving towards a more disabled profile and added years of life. Interventions should encourage self-care behaviors constituting a favorable pattern.
Extreme Precipitation in the Great Lakes Region: Trend Estimation and Relation With Large-Scale Circulation and Humidity
Authors: Paxton Andrew , Schoof Justin T. , Ford Trent W. , Remo Jonathan W. F.
Published: December 2021

Extreme precipitation contributes to widespread impacts in the U.S. Great Lakes region, ranging from agricultural losses to urban floods and associated infrastructure costs. Previous studies have reported historical increases in the frequency of extreme precipitation in the region and downscaled model projections indicate further changes as the climate system continues to warm. Here, we conduct trend analysis on the 5 km NOAA NClimDiv data for the U.S. Great Lakes region using both parametric (Ordinary Least Squares) and non-parametric methods (Theil-Sen/Mann-Kendall) and accounting for temporal autocorrelation and field significance to produce robust estimates of extreme precipitation frequency trends in the region. The approaches provide similar overall results and reflect an increase in extreme precipitation frequency in parts of the U.S. Great Lakes region. To relate the identified trends to large scale drivers, a bivariate self-organizing map (SOM) is constructed using standardized values of 500 hPa geo-potential height and 850 hPa specific humidity obtained from the ECMWF ERA-5 reanalysis. Using a Monte Carlo approach, we identify six SOM nodes that account for only 25.4% of all days, but 50.5% of extreme precipitation days. Composites of days with and without extreme precipitation for each node indicate that extreme events are associated with stronger features (height gradient and background humidity) than their non-extreme counterparts. The analysis also identifies a significant increase in the frequency of one SOM node often associated with extreme precipitation (accounting for 8.5% of all extreme precipitation days) and a significant increase in the frequency of extreme precipitation days relative to all days across the six extreme precipitation nodes collectively. Our results suggest that changes in atmospheric circulation and related moisture transport and convergence are major contributors to changes in extreme precipitation in the U.S. Great Lakes region.
Reduced Water Use in Barley and Maize Production Through Conservation Agriculture and Drip Irrigation
Authors: Fonteyne Simon , Flores García Álvaro , Verhulst Nele
Published: December 2021

The Mexican Bajío region is the country's main barley (Hordeum vulgare) producing area. Barley is commonly produced during the dry autumn–winter season using furrow irrigation with ground water, following which rainfed maize (Zea mays) is grown in the spring–summer season using supplementary irrigation. Ground water levels in the region are steadily dropping, and the introduction of water-saving technologies in agriculture is urgently required. Drip irrigation can reduce water use but is costly. Conservation agriculture—the combination of minimal tillage, permanent soil cover and crop diversification—might reduce water use, but studies in irrigated systems are scarce. We compared water use and grain yield in tillage-based conventional agriculture and conservation agriculture, both with furrow irrigation and drip irrigation, in a 3-year (six growing seasons) barley-maize field experiment. Additionally, side-by-side demonstrations of conventional and conservation agriculture were installed simultaneously in farmers' fields and yields, water use and fuel use were recorded. In the field experiment, yields did not differ significantly between production systems, but irrigation water use was on average 17% lower in conservation agriculture than in conventional agriculture, ~36% lower with drip irrigation compared with furrow irrigation in conventional tillage, and 40% lower with drip irrigation and conservation agriculture combined compared with conventional agriculture with furrow irrigation. Water use reductions differed strongly between years, depending on weather. The water saving through conservation agriculture in farmers' fields was similar to the water saving in the controlled experiment with about 17%. Additionally, in farmer's fields conservation agriculture reduced greenhouse gas emissions by 192 kg CO2 ha−1 and improved soil health. The implementation of conservation agriculture would be a cost-effective method to reduce water use in the barley-maize production system in the Mexican Bajío, while simultaneously reducing greenhouse gas emissions.
Endophyte Infected Tall Fescue: Plant Symbiosis to Animal Toxicosis
Authors: Ferguson Taylor D. , Vanzant Eric S. , McLeod Kyle R.
Published: December 2021

Endophyte-infected fescue is a major cool season forage used for livestock production in the United States and through other areas of the world. A unique aspect of this forage resource is the symbiotic relationship with an endophytic fungus (Epichloë coenophiala) that has detrimental impact on herbivores due to toxic ergot alkaloids. Research over the past 50 years has unveiled details regarding this symbiotic relationship. This review focuses on the origin of tall fescue in the United States and the consequences of its wide-spread utilization as a livestock forage, along with the discovery and toxicodynamics of ergot alkaloids produced byE. coenophiala. The majority of past ergot alkaloid research has focused on observing phenotypic changes that occur in livestock affected by ergot alkaloids, but recent investigation of the metabolome, transcriptome, and proteome have shown that fescue toxicity-related illnesses are much more complex than previous research suggests.
Integrated Analysis of mRNA and MicroRNA Co-expressed Network for the Differentiation of Bovine Skeletal Muscle Cells After Polyphenol Resveratrol Treatment
Authors: Hao Dan , Wang Xiao , Yang Yu , Thomsen Bo , Holm Lars-Erik , Qu Kaixing , Huang Bizhi , Chen Hong
Published: December 2021

Resveratrol (RSV) has been confirmed to benefit human health. Resveratrol supplemented in the feeds of animals improved pork, chicken, and duck meat qualities. In this study, we identified differentially expressed (DE) messenger RNAs (mRNAs) (n = 3,856) and microRNAs (miRNAs) (n = 93) for the weighted gene co-expression network analysis (WGCNA) to investigate the co-expressed DE mRNAs and DE miRNAs in the primary bovine myoblasts after RSV treatment. The mRNA results indicated that RSV treatments had high correlations with turquoise module (0.91, P-value = 0.01) and blue module (0.93, P-value < 0.01), while only the turquoise module (0.96, P-value < 0.01) was highly correlated with the treatment status using miRNA data. After biological enrichment analysis, the 2,579 DE genes in the turquoise module were significantly enriched in the Gene Ontology (GO) terms and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. The top two GO terms were actin filament-based process (GO:0030029) and actin cytoskeleton organization (GO:0030036). The top two KEGG pathways were regulation of actin cytoskeleton (bta04810) and tight junction (bta04530). Then, we constructed the DE mRNA co-expression and DE miRNA co-expression networks in the turquoise module and the mRNA–miRNA targeting networks based on their co-expressions in the key module. In summary, the RSV-induced miRNAs participated in the co-expression networks that could affect mRNA expressions to regulate the primary myoblast differentiation. Our study provided a better understanding of the roles of RSV in inducing miRNA and of the characteristics of DE miRNAs in the key co-expressed module in regulation of mRNAs and revealed new candidate regulatory miRNAs and genes for the beef quality traits.
A New Algorithm for Simultaneous Retrieval of Aerosols and Marine Parameters
Authors: Ssenyonga Taddeo , Frette Øyvind , Hamre Børge , Stamnes Knut , Muyimbwa Dennis , Ssebiyonga Nicolausi , Stamnes Jakob J.
Published: December 2021

We present an algorithm for simultaneous retrieval of aerosol and marine parameters in coastal waters. The algorithm is based on a radiative transfer forward model for a coupled atmosphere-ocean system, which is used to train a radial basis function neural network (RBF-NN) to obtain a fast and accurate method to compute radiances at the top of the atmosphere (TOA) for given aerosol and marine input parameters. The inverse modelling algorithm employs multidimensional unconstrained non-linear optimization to retrieve three marine parameters (concentrations of chlorophyll and mineral particles, as well as absorption by coloured dissolved organic matter (CDOM)), and two aerosol parameters (aerosol fine-mode fraction and aerosol volume fraction). We validated the retrieval algorithm using synthetic data and found it, for both low and high sun, to predict each of the five parameters accurately, both with and without white noise added to the top of the atmosphere (TOA) radiances. When varying the solar zenith angle (SZA) and retraining the RBF-NN without noise added to the TOA radiance, we found the algorithm to predict the CDOM absorption, chlorophyll concentration, mineral concentration, aerosol fine-mode fraction, and aerosol volume fraction with correlation coefficients greater than 0.72, 0.73, 0.93, 0.67, and 0.87, respectively, for 45∘≤ SZA ≤ 75∘. By adding white Gaussian noise to the TOA radiances with varying values of the signal-to-noise-ratio (SNR), we found the retrieval algorithm to predict CDOM absorption, chlorophyll concentration, mineral concentration, aerosol fine-mode fraction, and aerosol volume fraction well with correlation coefficients greater than 0.77, 0.75, 0.91, 0.81, and 0.86, respectively, for high sun and SNR ≥ 95.
Reproductive Technologies Used in Male Neo-Tropical Hystricomorphic Rodents
Authors: Lall Kavita Ranjeeta , Jones Kegan Romelle , Garcia Gary Wayne
Published: December 2021

This review, which is the first of two, focuses on the male reproductive anatomy and reproductive technologies used in Neo-tropical hystricomorphic rodents with the potential for domestication, which are the agouti (Dasyprocta leporina), the capybara (Hydrochoerus hydrochaeris) and the paca (Cuniculus paca). We consider over seventy references spanning from 1965 to 2020, with the majority of work being done in the past twenty years. Knowledge of the reproductive tract and reproductive technologies is critical to the conservation and preservation of these species. Although all three animals had similarities in their anatomy, such as no overt scrotums and testes located intra-abdominally in the inguinal region, some had unique features—for example, the agouti and the paca had penile spines, and two lateral penile cartilages. High spermatogenic efficiency was noted in the agouti and the paca, making them good candidates for increasing their reproductive performance in conservation programs. A review of the literature has shown that there is increasing work taking place on the reproductive technologies used in these animals; however, a lot of work is still lacking, as, to the author’s knowledge, standard protocols and artificial insemination procedures are yet to be established.
Lacticaseicin 30 and Colistin as a Promising Antibiotic Formulation against Gram-Negative β-Lactamase-Producing Strains and Colistin-Resistant Strains
Authors: Madi-Moussa Désiré , Belguesmia Yanath , Charlet Audrey , Drider Djamel , Coucheney Françoise
Published: December 2021

Antimicrobial resistance is a global health concern across the world and it is foreseen to swell if no actions are taken now. To help curbing this well announced crisis different strategies are announced, and these include the use of antimicrobial peptides (AMP), which are remarkable molecules known for their killing activities towards pathogenic bacteria. Bacteriocins are ribosomally synthesized AMP produced by almost all prokaryotic lineages. Bacteriocins, unlike antibiotics, offer a set of advantages in terms of cytotoxicity towards eukaryotic cells, their mode of action, cross-resistance and impact of microbiota content. Most known bacteriocins are produced by Gram-positive bacteria, and specifically by lactic acid bacteria (LAB). LAB-bacteriocins were steadily reported and characterized for their activity against genetically related Gram-positive bacteria, and seldom against Gram-negative bacteria. The aim of this study is to show that lacticaseicin 30, which is one of the bacteriocins produced by Lacticaseibacillus paracasei CNCM I-5369, is active against Gram-negative clinical strains (Salmonella enterica Enteritidis H10, S. enterica Typhimurium H97, Enterobacter cloacae H51, Escherichia coli H45, E. coli H51, E. coli H66, Klebsiella oxytoca H40, K. pneumoniae H71, K. variicola H77, K. pneumoniae H79, K. pneumoniae H79), whereas antibiotics failed. In addition, lacticaseicin 30 and colistin enabled synergistic interactions towards the aforementioned target Gram-negative clinical strains. Further, the combinations of lacticaseicin 30 and colistin prompted a drastic downregulation of mcr-1 and mcr-9 genes, which are associated with the colistin resistance phenotypes of these clinical strains. This report shows that lacticaseicin 30 is active against Gram-negative clinical strains carrying a rainbow of mcr genes, and the combination of these antimicrobials constitutes a promising therapeutic option that needs to be further exploited.