bluuebunny/crossref_metadata_2025 huggingface dataset:
Polychromy in Ancient Greek Sculpture: New Scientific Research on an Attic Funerary Stele at the Metropolitan Museum of Art
Authors: Basso Elena , Carò Federico , Abramitis Dorothy H.
Published: February 2023

Polychromy in Ancient Greek Sculpture was the subject of the exhibition Chroma: Ancient Greek Sculpture in Color, held at The Metropolitan Museum of Art (The Met), New York, in 2022–2023. On this occasion, a multidisciplinary project involving The Met’s Departments of Greek and Roman Art, Objects Conservation, Imaging, Scientific Research, and colleagues from the Liebieghaus Polychromy Research Project in Frankfurt, Germany, was carried out to study an Attic funerary monument. The color decoration of the sphinx was reconstructed by combining non-invasive and minimally invasive techniques that provided information about surviving and lost pigments, original design, and painting technique. Results of multiband imaging, digital microscopy, and X-ray fluorescence spectroscopy guided the removal of minute samples from selected areas for examination by Raman spectroscopy and scanning electron microscopy, coupled with energy-dispersive X-ray spectroscopy, to shed light on the pigments and paint stratigraphy. The color palette included two varieties of blue, Egyptian blue and azurite, a carbon-based black pigment, two reds, cinnabar and red ocher, and yellow ocher, all painted directly over the marble without a preparation layer. The scientific findings informed the physical reconstruction of the sphinx made by archaeologists from the Liebieghaus Polychromy Research Project, featured in the exhibition.
Study of the Interaction between Regular Waves and a Wind-Fish Combined Structure
Authors: Zhao Zilong , Yu Wenzhi , Xu Tiaojian
Published: February 2023

The combination of a wind turbine and fish cage (wind-fish) is able to solve the complex issue of power supply for deep-sea aquaculture. Nevertheless, the hydrodynamic coupling between the fish cage and the wind turbine foundation is increasingly complex, and there is little research on the related hydrodynamics. In this paper, the hydrodynamic research on the wind turbine foundation and fish cage structure is carried out using numerical simulation, in which the porous medium model is adopted to simulate the effects of the fish cage on the flow field. By analyzing the hydrodynamic characteristics of the combined structure of an offshore wind turbine and aquaculture cage under the action of regular waves, the load on both the cage in the combined structure and the wind turbine foundation, and the overall load on the combined structure of offshore wind turbine and cage are obtained. The effects of wave parameters, structural dimensions, cage submergence depth, and biological attachment on cage load, wind turbine foundation load, and overall load of the combined structure are analyzed. The results demonstrate that the wind turbine foundation is persistently the central part of the combined structure. The wave height, water depth, and degree of attachment of marine organisms all have different effects on the amplitude of the structural load. In contrast, the wave period, cage geometry, and cage submergence depth have no significant effects on the amplitude of the structural load. In addition to the water depth, other factors will affect the proportion of the load on the wind turbine foundation and the total load as well.
Classification and Segmentation of Diabetic Retinopathy: A Systemic Review
Authors: Shaukat Natasha , Amin Javeria , Sharif Muhammad Imran , Sharif Muhammad Irfan , Kadry Seifedine , Sevcik Lukas
Published: February 2023

Diabetic retinopathy (DR) is a major reason of blindness around the world. The ophthalmologist manually analyzes the morphological alterations in veins of retina, and lesions in fundus images that is a time-taking, costly, and challenging procedure. It can be made easier with the assistance of computer aided diagnostic system (CADs) that are utilized for the diagnosis of DR lesions. Artificial intelligence (AI) based machine/deep learning methods performs vital role to increase the performance of the detection process, especially in the context of analyzing medical fundus images. In this paper, several current approaches of preprocessing, segmentation, feature extraction/selection, and classification are discussed for the detection of DR lesions. This survey paper also includes a detailed description of DR datasets that are accessible by the researcher for the identification of DR lesions. The existing methods limitations and challenges are also addressed, which will assist invoice researchers to start their work in this domain.
Artistic Methodologies in Forced Migration: Using Body Mapping and Augmented Reality in Syrian Refugees’ Narratives
Authors: Shanneik Yafa , Sobieczky Elisabeth
Published: February 2023

Millions of refugees fleeing war and persecution in Syria have received significant attention in both the public sphere and academic research. This article examines body mapping and augmented reality (AR) as artistic methodological tools that allow refugees to articulate their experiences of forced migration. Body mapping enables the creation of life-sized images which trace the contours of the individual’s body on canvas allowing refugees to express their emotions creatively through their own participation. AR adds another layer to the body map as it animates the artwork and brings it to life. Through body mapping and AR, refugee women from Syria are able to co-create research outcomes and counter gender-biased narratives of vulnerability and victimhood often associated with female Muslim refugees. This project critically engages with the use of art as a sensorialized medium to generate knowledge and examines the impact it shows on viewers during exhibitions. Based on research conducted in the United Kingdom, Germany and Jordan since 2017, this article discusses the use of these new technologies as novel research methodologies in refugee and migration studies.
Aerosols as Vectors for Contaminants: A Perspective Based on Outdoor Aerosol Data from Kuwait
Authors: Uddin Saif , Habibi Nazima , Fowler Scott W. , Behbehani Montaha , Gevao Bondi , Faizuddin Mohammad , Gorgun Aysun Ugur
Published: February 2023

The Middle East is a hot spot of dust, and there are reports of as much as 1400 µg m−3 of dust in aerosols from Kuwait, which is among some of the highest dust loadings globally. A significant volume of literature has emerged on dust–air-quality–human-health, and the World Health Organization in its recent air quality guidelines has lowered the limit of annual PM2.5 exposure to 5 μg m−3 from the previous limit of 10 μg m−3. We present a mini-review based on a screening and search of the published data generated in Kuwait on contaminants associated with dust in different size fractions. We also include an unpublished study on organic contaminants in size-fractionated aerosols. The ΣPAHs concentrations in all the six size fractions range between 570 and 3350 pg m−3. The ∑PBDE concentration ranges from ~2 to 1307 pg m−3. The average 210Po activity in aerosol size classes varies between 2289 and 2581 Bq kg−1. The average 210Pb concentration varies between 352 and 412 Bq kg−1. The MP inventory in Kuwait’s outdoor aerosol is between 5 and 35 MP in 815 ± 5 m3 of air. The bacterial load in outdoor aerosols is between 6.05 × 103 cells m−3 and 1.24 × 108 cells m−3. The fungal load ranges between 2.11 × 102 cells m−3 and 2.66 × 106 cells m−3. The data suggest that the inhalable fraction of <2.5 µm size contains high concentrations of Polycyclic Aromatic Hydrocarbons (PAHs), Polybrominated diphenyl ethers (PBDEs), 210Po, 210Pb, microplastics, and microbes. These enriched ultrafine aerosols pose a significant risk to human health. The review also highlights the scarcity of contaminant data in respirable and inhalable size fractions that are critical for a comprehensive inhalation risk assessment.
Quantitative Assessment of Airborne Transmission of Human and Animal Influenza Viruses in the Ferret Model
Authors: Gudymo Andrey , Onkhonova Galina , Danilenko Alexey , Susloparov Ivan , Danilchenko Natalia , Kosenko Maxim , Moiseeva Anastasia , Kolosova Natalia , Svyatchenko Svetlana , Marchenko Vasily , Nhai Tran Thi , Kuznetsov Andrey , Gavrilova Elena , Maksyutov Rinat , Ryzhikov Alexander
Published: February 2023

The WHO has developed a tool to assess the risk of newly emerging influenza viruses with pandemic potential (TIPRA). According to TIPRA, the main parameters for assessing the risk of human-to-human transmission of a novel influenza virus are its ability to bind to human cell receptors of the upper respiratory tract (URT) and transmit in model animals. The aim of this study was to quantify airborne transmission of human and animal influenza viruses in the ferret model. The transmission of influenza viruses was studied in the ferret model in an aerobiology chamber. Airborne particles concentration and fractional composition in the aerobiology chamber were measured using an aerosol particle counter and analytical aerosol filters. Viral load in ferret nasal washings and aerosol filters was determined by titration in MDCK cells and quantitative RT-PCR. Genetic analysis of influenza viruses was performed using virus genome sequences obtained by NGS. After intranasal infection, human and animal influenza viruses replicated in the cells of nasal mucosa in ferrets. The level of virus airborne particles contamination provided by infected animals depends on the infectious dose and differs significantly between influenza virus strains. The studied avian influenza viruses show insufficient transmission in the ferret model, while human and swine influenza viruses are highly transmitted in ferrets. We propose a quantitative model of airborne transmission of influenza virus from donor to recipient ferrets. Level of influenza virus transmission in the ferret model correlates with genetic markers of virus receptor specificity and the level of virus airborne particle contamination induced by donor ferrets.
Predicting Air Quality from Measured and Forecast Meteorological Data: A Case Study in Southern Italy
Authors: Tateo Andrea , Campanaro Vincenzo , Amoroso Nicola , Bellantuono Loredana , Monaco Alfonso , Pantaleo Ester , Rinaldi Rosaria , Maggipinto Tommaso
Published: February 2023

A great deal of attention has been devoted to the analysis of particulate matter (PM) concentrations in various scenarios because of their negative effects on human health. Here, we investigate how meteorological conditions can affect PM concentrations in the peculiar case of the district of the city of Lecce in the Apulia region (Southern Italy), which is characterized by the highest tumor rate of the whole region despite the absence of nearby heavy industries. We present a unified machine learning framework which combines air quality and meteorological data, either measured on ground or forecast. Our findings show that the concentrations of PM10, PM2.5, NO2 and CO are significantly associated with the meteorological conditions and suggest that it is possible to predict air quality using either ground weather observations or weather forecasts.
Three-Dimensional Printing of Poly-L-Lactic Acid Composite Scaffolds with Enhanced Bioactivity and Controllable Zn Ion Release Capability by Coupling with Carbon-ZnO
Authors: Yuan Xun , Zhu Wei , Yang Zhongyuan , Chen Feng , Han Xiaoxiao
Published: February 2023

Poly-L-lactic acid (PLLA) has gained great popularity with researchers in regenerative medicine owing to its superior biocompatibility and biodegradability, although its inadequate bioactivity inhibits the further use of PLLA in the field of bone regeneration. Zinc oxide (ZnO) has been utilized to improve the biological performance of biopolymers because of its renowned osteogenic activity. However, ZnO nanoparticles tend to agglomerate in the polymer matrix due to high surface energy, which would lead to the burst release of the Zn ion and, thus, cytotoxicity. In this study, to address this problem, carbon–ZnO (C–ZnO) was first synthesized through the carbonization of ZIF-8. Then, C–ZnO was introduced to PLLA powder before it was manufactured as scaffolds (PLLA/C–ZnO) by a selective laser sintering 3D printing technique. The results showed that the PLLA/C–ZnO scaffold was able to continuously release Zn ions in a reasonable range, which can be attributed to the interaction of Zn–N bonding and the shielding action of the PLLA scaffold. The controlled release of Zn ions from the scaffold further facilitated cell adhesion and proliferation and improved the osteogenic differentiation ability at the same time. In addition, C–ZnO endowed the scaffold with favorable photodynamic antibacterial ability, which was manifested by an efficient antibacterial rate of over 95%.
Age-Related In Vivo Structural Changes in the Male Mouse Olfactory Bulb and Their Correlation with Olfactory-Driven Behavior
Authors: Bontempi Pietro , Ricatti Maria Jimena , Sandri Marco , Nicolato Elena , Mucignat-Caretta Carla , Zancanaro Carlo
Published: February 2023

Olfactory areas in mammalian brains are linked to centers that modulate behavior. During aging, sensitivity to odors decreases and structural changes are described in olfactory areas. We explored, in two groups of male mice (young and elderly, 6 and 19 months old, respectively), the link between the changes in olfactory bulb structure, detected with magnetic resonance imaging, and behavioral changes in a battery of tests on motor, olfactory, cognitive performance, and emotional reactivity. The behavioral pattern of elderly mice appears less anxious, being less scared by new situations. Additionally, the olfactory bulb of young and elderly mice differed in two variables derived from magnetic resonance imaging (fractional anisotropy and T2 maps). A random forest approach allowed to select the variables most predictive of the differences between young and elderly mice, and correlations were found between three behavioral variables indicative of anxious behavior and the two magnetic resonance variables mentioned above. These data suggest that in the living mouse, it is possible to describe co-occurring age-related behavioral and structural changes in the olfactory bulb. These data serve as a basis for studies on normal and pathological aging in the mouse, but also open new opportunities for in vivo human aging studies.
Lahaul–Zanskar–Sham Valley Corridor in Indian Trans Himalayan Region Facilitates Dispersal and Gene Flow in Himalayan Ibex
Authors: Jabin Gul , Dolker Stanzin , Joshi Bheem Dutt , Singh Sujeet Kumar , Chandra Kailash , Sharma Lalit Kumar , Thakur Mukesh
Published: February 2023

Wildlife corridors that connect mosaic habitats in heterogeneous mountainous landscapes can be of high significance as they facilitate the genetic and demographic stability of free-ranging populations. Peripheral populations of widespread species are usually ignored in conservation planning. However, these populations retain locally common alleles and are genetic reservoir under the changing climatic conditions. Capra sibirica has widespread distribution, and its southern peripheral population is distributed in the Indian trans-Himalayan region (ITR). In the present study, we studied the spatial distribution and genetic make-up of Himalayan ibex from the ITR following the landscape genetics approach. We obtained 16 haplotypes at the mitochondrial d-loop region and found a stable demography in the past with a recent decline. With 10 nuclear microsatellites, we ascertained 111 unique individuals assigned into two clusters following Bayesian and non-Bayesian clustering analysis with several admixed individuals. We also recorded 25 first-generation migrants that reflected relatively high dispersal and gene-flow across the range. We identified a 19,835 sq.km suitable area with 13,311 sq.km in Ladakh and 6524 sq.km in Lahaul-Spiti. We identified a novel movement corridor for Himalayan ibex across the Lahaul–Zanskar–Sham valley (L–Z–SV) that displayed a fairly good conductance with low genetic divergence among the samples collected on the L–Z–SV corridor. We propose declaring a protected area in the Lahaul and Kargil districts to prioritize dedicated conservation efforts for the Himalayan ibex and other sympatric ungulates that impart a major role in the diet of large carnivore and balancing ecosystem services in the trans-Himalayan region.
Circle(s) of Life: The Circadian Clock from Birth to Death
Authors: Olejniczak Iwona , Pilorz Violetta , Oster Henrik
Published: February 2023

Most lifeforms on earth use endogenous, so-called circadian clocks to adapt to 24-h cycles in environmental demands driven by the planet’s rotation around its axis. Interactions with the environment change over the course of a lifetime, and so does regulation of the circadian clock system. In this review, we summarize how circadian clocks develop in humans and experimental rodents during embryonic development, how they mature after birth and what changes occur during puberty, adolescence and with increasing age. Special emphasis is laid on the circadian regulation of reproductive systems as major organizers of life segments and life span. We discuss differences in sexes and outline potential areas for future research. Finally, potential options for medical applications of lifespan chronobiology are discussed.
Non-Invasive Assessment of Vascular Circulation Based on Flow Mediated Skin Fluorescence (FMSF)
Authors: Marcinek Andrzej , Katarzynska Joanna , Sieron Leslaw , Skokowski Robert , Zielinski Jacek , Gebicki Jerzy
Published: February 2023

Flow Mediated Skin Fluorescence (FMSF) is a new non-invasive method for assessing vascular circulation and/or metabolic regulation. It enables assessment of both vasoconstriction and vasodilation. The method measures stimulation of the circulation in response to post-occlusive reactive hyperemia (PORH). It analyzes the dynamical changes in the emission of NADH fluorescence from skin tissue, providing the information on mitochondrial metabolic status and intracellular oxygen delivery through the circulatory system. Assessment of the vascular state using the FMSF technique is based on three parameters: reactive hyperemia response (RHR), hypoxia sensitivity (HS), and normoxia oscillatory index (NOI). The RHR and HS parameters determine the risk of vascular circulatory disorders and are the main diagnostic parameters. The NOI parameter is an auxiliary parameter for evaluating the state of microcirculation under stress of various origins (e.g., emotional stress, physical exhaustion, or post-infection stress). The clinical data show that the risk of vascular complications is limited among people whose RHR, log(HS), and NOI parameters are not significantly below the mean values determined by the FMSF technique, especially if they simultaneously meet the conditions RHR > 30% and log(HS) > 1.5 (HS > 30), and NOI > 60%.
Intestinal Barrier in Post-Campylobacter jejuni Irritable Bowel Syndrome
Authors: Omarova Sholpan , Awad Karem , Moos Verena , Püning Christoph , Gölz Greta , Schulzke Jörg-Dieter , Bücker Roland
Published: February 2023

Background: Campylobacter jejuni (C. jejuni) is one of the most common causes of bacterial gastroenteritis worldwide. One sequela of this infection is the development of post-infectious irritable bowel syndrome (PI-IBS). It has been suggested that a dysfunctional intestinal barrier may promote IBS development. We aimed to test this hypothesis against the background of the leaky gut concept for low-grade inflammation in PI-IBS. Methods: We identified patients with persistent PI-IBS symptoms after C. jejuni infection. During sigmoidoscopy, forceps biopsies were obtained for electrophysiological measurements of epithelial transport and barrier function in miniaturized Ussing devices. C. jejuni absence was checked by PCR and cytokine production with immunohistochemistry. Results: In PI-IBS, the epithelial resistance of the colon epithelium was unaltered, reflecting an intact paracellular pathway. In contrast, temperature-dependent horseradish peroxidase (HRP, 44 kDa) permeation increased. Short-circuit current (Isc) reflecting active anion secretion and ENaC-dependent electrogenic sodium absorption was unaffected. Early endosome antigen-1 (EEA1) and IL-4 levels increased. C. jejuni is not incorporated into the resident microbiota of the colon mucosa in PI-IBS. Conclusions: In PI-IBS after C. jejuni infection, macromolecule uptake via endocytosis was enhanced, leading to low-grade inflammation with pro-inflammatory cytokine release. The findings will allow C. jejuni-induced pathomechanisms to be targeted during infection and, thereafter to reduce sequelae such as PI-IBS.
Natural IgG Anti-F (ab’)2 Autoantibody Activity in Children with Autism
Authors: Tordjman Sylvie , Charrier Annaëlle , Kazatchkine Michel , Roubertoux Pierre , Botbol Michel , Bronsard Guillaume , Avrameas Stratis
Published: February 2023

Background: Many and diverse autoimmune abnormalities have been reported in children with autism. Natural autoantibodies (NAAbs) play important immunoregulatory roles in recognition of the immune self. The objective of this study was to examine the presence of NAAbs in the sera of children with autism and across severity subgroups of autistic behavioral impairments. Methods: NAAbs were titrated in sera through an ELISA procedure in 60 low-functioning children with autism and 112 typically developing controls matched for age, sex and puberty. Results: Serum titers of IgG anti-F(ab’)2 autoantibodies were significantly lower in children with autism compared to typically developing controls (p < 0.0001), and were significantly negatively associated with autism severity (p = 0.0001). This data appears to be related more specifically to autism than to intellectual disability, given that IgG anti-F(ab’)2 levels were significantly negatively correlated with IQ scores in the autism group (p = 0.01). Conclusions: This is the first report in autism of abnormally low natural anti-F(ab’)2 autoantibody activity. The findings suggest a dysfunction of self-recognition mechanisms which may play a role in the pathogenesis of autism, especially for the severely affected children. These findings strengthen the hypothesis of an autoimmune process in autism and open the prospect of alternative medical treatment. Further neuroimmunological research is warranted to understand the exact mechanisms underlying this reduced natural IgG anti-F (ab’)2 autoantibody activity, and to assess its impact on the pathophysiology and behavioral expression of autism.
Overcoming Barriers to Wound Healing in a Neuropathic and Neuro-Ischaemic Diabetic Foot Cohort Using a Novel Bilayer Biodegradable Synthetic Matrix
Authors: Guerriero Frank P. , Clark Robyn A. , Miller Michelle , Delaney Christopher L.
Published: February 2023

Diabetes-related foot ulceration presents an increasing risk of lower limb amputation globally, driving the search for new treatment technologies. Our single-centre prospective observational study reports on the impact of bilayer biodegradable synthetic matrix technology (NovoSorb® BTM) on the healing and amputation rates of a diabetic foot ulceration cohort. Consecutive patients with a diabetes-related foot ulceration treated with NovoSorb BTM, between December 2019 and October 2021, were followed for 12 months. Complete wound healing and amputation outcomes were observed. Amputation risk was stratified using the Wound, Ischaemia and foot Infection (WIfI) classification system. Study outcomes were compared with recently published meta-analysis data to evaluate the impact of the synthetic matrix. In total, 25 NovoSorb BTM applications to 23 wounds in 22 patients were observed. Complete wound healing was observed in 15 of the wounds, 3 retained chronic wounds, 3 required minor amputation and 2 required major limb amputation. Further, 12-month WIfI amputation risk analysis saw 18 patients stratified to WIfI stage 4, 4 to WIfI stage 3 and 1 to WIfI stage 1. Our observed 12-month major amputation rates were 11.1% (n = 2) for stage 4 and 0% for stages 3 and 1. Our early experience suggests that NovoSorb BTM is a safe and effective treatment for moderate to severe diabetes-related foot ulceration. While larger-scale data are required, NovoSorb BTM may represent a promising new addition to the armamentarium of clinicians, who strive to achieve limb salvage in this complex cohort of patients.
Michael Adduct of Sulfonamide Chalcone Targets Folate Metabolism in Brugia Malayi Parasite
Authors: Bhoj Priyanka S. , Bahekar Sandeep P. , Chowdhary Shambhavi , Togre Namdev S. , Amdare Nitin P. , Jena Lingaraj , Goswami Kalyan , Chandak Hemant
Published: February 2023

A series of Michael adducts of malononitrile and sulfonamide chalcones were synthesized, characterized, and evaluated for their antifilarial activity. Out of 14 compounds, N-(4-(4,4-dicyano-3-p-tolylbutanoyl)phenyl)benzenesulfonamide showed favorable drug-likeness properties with marked antifilarial effects at micro-molar dosages. Apoptosis in Brugia malayi microfilariae was confirmed by EB/AO staining, MTT assay, and cytoplasmic cytochrome c ELISA. Since chalcone and folate synthesis pathways share the same substrate, we hypothesize a structural analogy-based inhibition of folate metabolism by this compound. Molecular docking against a pre-validated BmDHFR protein showed more favorable thermodynamic parameters than a positive control, epicatechin-3-gallate. The compound significantly suppressed the DHFR activity in a parasite extract in vitro. Our hypothesis is also supported by a significant reversal of DHFR inhibition by folate addition, which indicated a plausible mechanism of competitive inhibition. These results demonstrate that targeting filarial folate metabolism through DHFR with consequent apoptosis induction might be rewarding for therapeutic intervention. This study reveals a novel rationale of the structural analogy-based competitive inhibition of DHFR by Michael adducts of sulfonamide chalcones.
Telomere Shortening in Three Diabetes Mellitus Types in a Mexican Sample
Authors: Cuevas Diaz Pavel , Nicolini Humberto , Nolasco-Rosales German Alberto , Juarez Rojop Isela , Tovilla-Zarate Carlos Alfonso , Rodriguez Sanchez Ester , Genis-Mendoza Alma Delia
Published: February 2023

This study aimed to explore the role of telomere length in three different diabetes types: latent autoimmune diabetes of adulthood (LADA), latent autoimmune diabetes in the young (LADY), and type 2 diabetes mellitus (T2DM). A total of 115 patients were included, 72 (62.61%) had LADA, 30 (26.09%) had T2DM, and 13 (11.30%) had LADY. Telomere length was measured using real-time Polymerase Chain Reaction. For statistical analysis, we used the ANOVA test, X2 test, and the Mann–Whitney U test. Patients with T2DM had higher BMI compared to LADA and LADY groups, with a BMI average of 31.32 kg/m2 (p = 0.0235). While the LADA group had more patients with comorbidities, there was not a statistically significant difference (p = 0.3164, p = 0.3315, p = 0.3742 for each of the previously mentioned conditions). There was a difference between those patients with T2DM who took metformin plus any other oral antidiabetic agent and those who took metformin plus insulin, the ones who had longer telomeres. LADA patients had shorter telomeres compared to T2DM patients but not LADY patients. Furthermore, T2DM may have longer telomeres thanks to the protective effects of both metformin and insulin, despite the higher BMI in this group.
Odorant Binding Causes Cytoskeletal Rearrangement, Leading to Detectable Changes in Endothelial and Epithelial Barrier Function and Micromotion
Authors: Curtis Theresa M. , Nilon Annabella M. , Greenberg Anthony J. , Besner Matthew , Scibek Jacob J. , Nichols Jennifer A. , Huie Janet L.
Published: February 2023

Non-olfactory cells have excellent biosensor potential because they express functional olfactory receptors (ORs) and are non-neuronal cells that are easy to culture. ORs are G-protein coupled receptors (GPCRs), and there is a well-established link between different classes of G-proteins and cytoskeletal structure changes affecting cellular morphology that has been unexplored for odorant sensing. Thus, the present study was conducted to determine if odorant binding in non-olfactory cells causes cytoskeletal changes that will lead to cell changes detectable by electric cell-substrate impedance sensing (ECIS). To this end, we used the human umbilical vein endothelial cells (HUVECs), which express OR10J5, and the human keratinocyte (HaCaT) cells, which express OR2AT4. Using these two different cell barriers, we showed that odorant addition, lyral and Sandalore, respectively, caused an increase in cAMP, changes in the organization of the cytoskeleton, and a decrease in the integrity of the junctions between the cells, causing a decrease in cellular electrical resistance. In addition, the random cellular movement of the monolayers (micromotion) was significantly decreased after odorant exposure. Collectively, these data demonstrate a new physiological role of olfactory receptor signaling in endothelial and epithelial cell barriers and represent a new label-free method to detect odorant binding.
Electrochemical Sensing of Urinary Chloride Ion Concentration for Near Real-Time Monitoring
Authors: Nelson Anna M. , Habibi Sanaz , DeLancey John O. L. , Ashton-Miller James A. , Burns Mark A.
Published: February 2023

Urinary chloride concentration is a valuable health metric that can aid in the early detection of serious conditions, such as acid base disorders, acute heart failure, and incidences of acute renal failure in the intensive care unit. Physiologically, urinary chloride levels frequently change and are difficult to measure, involving time-consuming and inconvenient lab testing. Thus, near real-time simple sensors are needed to quickly provide actionable data to inform diagnostic and treatment decisions that affect health outcomes. Here, we introduce a chronopotentiometric sensor that utilizes commercially available screen-printed electrodes to accurately quantify clinically relevant chloride concentrations (5–250 mM) in seconds, with no added reagents or electrode surface modification. Initially, the sensor’s performance was optimized through the proper selection of current density at a specific chloride concentration, using electrical response data in conjunction with scanning electron microscopy. We developed a unique swept current density algorithm to resolve the entire clinically relevant chloride concentration range, and the chloride sensors can be reliably reused for chloride concentrations less than 50 mM. Lastly, we explored the impact of pH, temperature, conductivity, and additional ions (i.e., artificial urine) on the sensor signal, in order to determine sensor feasibility in complex biological samples. This study provides a path for further development of a portable, near real-time sensor for the quantification of urinary chloride.
The Latest Cellular and Molecular Mechanisms of COVID-19 on Non-Lung Organs
Authors: Askari Hamid , Rabiei Fatemeh , Lohrasbi Fatemeh , Ghadir Sara , Ghasemi-Kasman Maryam
Published: February 2023

Understanding the transmission pathways of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) will aid in developing effective therapies directed at the virus’s life cycle or its side effects. While severe respiratory distress is the most common symptom of a coronavirus 2019 (COVID-19) infection, the virus is also known to cause damage to almost every major organ and system in the body. However, it is not obvious whether pathological changes in extra-respiratory organs are caused by direct infection, indirect, or combination of these effects. In this narrative review, we first elaborate on the characteristics of SARS-CoV-2, followed by the mechanisms of this virus on various organs such as brain, eye, and olfactory nerve and different systems such as the endocrine and gastrointestinal systems.
Information Exchange between Construction and Manufacturing Industries to Achieve Circular Economy: A Literature Review and Interviews with Swedish Experts
Authors: Moscati Annika , Johansson Peter , Kebede Rahel , Pula Amy , Törngren Annie
Published: February 2023

The construction industry is responsible for 32% of the world’s consumption of natural resources, causing 850 million tons of construction waste in the European Union alone. The construction and manufacturing industries mostly apply a linear economy model, where resources are used once and then disposed of. As a response, the concept of a circular economy (CE) was introduced with the aim of keeping materials within a closed loop and regaining their value in the end-of-life phase. This paper aims to investigate the different CE strategies in the construction industry and the related manufacturing industry and how these strategies can be supported by digital technology. The 11Rs framework has been used as a reference within the concept of CE through a scoping literature review, in which 117 articles were reviewed. The analysis focused on the frequency of the various Rs in papers, as well as their applications and possible implementation. Seven interviews were conducted with Swedish experts to obtain industry perspectives and thereby specify a potential research gap related to CE. According to the study, existing research for the application of CE by construction companies has not been fully explored. There is also a lack of knowledge about CE and its benefits, as well as poor collaboration between different stakeholders, both in information sharing and processes. Findings suggest that all stakeholders in the industries need to make efforts to change their strategies if the transition to CE is to occur.
Different Approach for the Structure Inclination Determination
Authors: Bačová Daša , Ižvoltová Jana , Šedivý Štefan , Chromčák Jakub
Published: February 2023

The current engineering and building pace has reached localities where vast civil projects were not considered. The changes of the intravillan area may cause some vacant historical localities to become a boundary or even a part of occupied area. The proximity of designed civil projects to historical structures may have great impact on their stability, and it is recommended or even legislatively set to monitor the possible changes in their shape or position. In case of protected structures, it is convenient to find a non-invasive way to measure and monitor historical structures if possible. Many data acquisition methods used in civil engineering for various purposes have gone through significant technological progress and enable the new ways of data collection. It is needed to focus on these methods from an application and precision point of view.
Damage Detection in Nonlinear Elastic Structures Using Individual Sensors
Authors: Quqa Said , Landi Luca
Published: February 2023

Natural frequencies have always been one of the most intuitive and widely used features for damage identification in civil structures. Even with the recent rapid technological and theoretical developments, frequency-based identification methods are of great interest for applications through low-cost sensing systems. Although most techniques for frequency identification assume a linear structural behavior, in real applications, variations in the amplitude of input excitation can lead to short-term frequency fluctuations due to the inherent nonlinearities of civil structures. This paper proposes a procedure for damage detection in nonlinear systems based on instantaneous resonant frequency and amplitude estimates. A statistical model was fitted to identified data, and a synthetic indicator was proposed to obtain robust damage detection, even when frequency shifts due to variations in the input excitation are comparable to those due to actual damage. The proposed method was applied to a dataset recorded from a reinforced concrete building with strongly nonlinear behavior.
Socio-Spatial Experience in Space Syntax Research: A PRISMA-Compliant Review
Authors: Lee Ju Hyun , Ostwald Michael J. , Zhou Ling
Published: February 2023

Characterising and predicting socio-spatial experience has long been a key research question in space syntax research. Due to the lack of synthesised knowledge about it, this review conducts the first systematic scoping review of space syntax research on the relationships between spatial properties and experiential values. Adopting the “Preferred Reporting Items for Systematic reviews and Meta-Analyses” (PRISMA) framework, this review of space syntax research identifies 38 studies that examine socio-spatial experiences in architectural, medical, and urban spaces. The data arising from this systematic review are used to identify trends in this sub-field of research, including the growth of socio-spatial methods and applications in urban analytics since 2016 and key methodological approaches, characteristics, and factors in space syntax research about socio-spatial experience. The research identified using the systematic framework employs a mixture of descriptive, correlation, and regression methods to examine the dynamic effects of spatial configurations on human experiences. Arising from the results of the review, the article further identifies a collective, predictive model consisting of five syntactic predictors and three categories of experiential values. This article, finally, examines research gaps and limitations in the body of knowledge and suggests future research directions.
Deterioration Performance of Recycled Aggregate Pervious Concrete under Freezing–Thawing Cycle and Chloride Environment
Authors: Chen Chen , Zhang Kai , Yin Zhigang , Zhou Jing
Published: February 2023

To study the performance of recycled aggregate pervious concrete (RAPC) in severe cold regions, the influence of the water–binder ratio, design porosity, micro silica fume (SF) and enhancer agent (EA) on the permeability and mechanical properties of the RAPC were evaluated using the orthogonal design method. Based on the optimal mixture proportion, freeze–thaw (F-T) experiments were carried out to investigate the effect of different media (water, 3.5 wt% NaCl solution) on the mechanical properties, permeability and anti-frost durability of the RAPC. Moreover, the porosity and pore structure characteristics of RAPC were evaluated after frost damage using an X-ray CT technique. The results indicate that the influence of the salt solution on the deterioration of the RAPC specimen is greater than water. The deterioration degree of the mass loss ratio, relative dynamic elastic modulus (RDEM), average compressive strength and porosity of the RAPC increase with increasing F–T cycles. Based on the two-parameter Weibull probabilistic distribution function, damage evolution models of RAPC are established, which can be used to quantitatively characterize the damage degree of the RAPC specimens under different F–T media. The results obtained can provide an experimental reference and data support for the promotion and application of RAPC pavement in severe cold regions.
Prestressed Steel-Concrete Composite I-Beams with Single and Double Corrugated Web
Authors: Nawar Mahmoud T. , El-Zohairy Ayman , Maaly Hassan M. , Husain Mohammed , Salama Islam , Mousa Eslam
Published: February 2023

Composite steel girders with concrete have been used for many years and advances in structural and fabrication technology have established their optimization. One of the changes in structural steel I-beams during the past few years has been the availability of web corrugation. The economic design of steel girders normally requires thin webs. Moreover, using externally prestressed tendons as a strengthening technique controls deflections and stresses. However, this strengthening technique causes shear buckling of the steel beams. In this study, the flexural behavior of externally prestressed composite steel-concrete I-beams with a single and double corrugated web was experimentally and numerically investigated. Three simply supported prestressed steel-concrete composite I-beams with single corrugated web (SCW) and double corrugated web (DCW) were tested under four-point loading. The tested beams were externally prestressed by using straight tendons along the full length. The experimental results showed that using SCW was more efficient in shear buckling resistance than DCW with the same equivalent web thickness. The ABAQUAS package was used to simulate the nonlinear behavior of the tested beams. The developed model was validated against the experimental results to carry out a parametric study in order to investigate the effect of various parameters on the behavior of the composite beams with SCW and DCW. Using stiffeners at the loading points as deviators to maintain the prestressed tendon positions increased the beam capacity and improved the beam performance.
Influence of Friction Coefficient between Cable and Membrane on Wind-Induced Response of Air-Supported Membrane Structures with Oblique Cable Net
Authors: Lai Guangxin , He Yanli , Zhao Yanguo , Zhang Limei
Published: February 2023

Cable-membrane constructions typically exhibit significant displacement and distortion under load action, and the cable-membrane contact results in relative sliding. In this paper, the interaction force between cable and membrane is transferred through frictional contact action, and as a result, the friction coefficient must be taken into consideration for the form-finding and load analysis of air-supported membrane structures. Eight kinds of P-type membrane materials commonly used in engineering were chosen, and the friction coefficients between the various membrane and cable materials were obtained. The connection between a cable and a membrane of an air-supported membrane structure is considered a contact problem, and its initial shape analysis and wind load response analysis are carried out. The influence of the friction coefficient of a cable membrane on the wind-induced response of an air-supported membrane structure is discussed, and the results are compared with those of the cable-membrane binding model under wind load.
Current Treatment Options and the Role of Functional Status Assessment in Classical Hodgkin Lymphoma in Older Adults: A Review
Authors: Zilioli Vittorio Ruggero , Muzi Cristina , Pagani Chiara , Ravano Emanuele , Meli Erika , Daffini Rosa , Ravelli Erika , Cairoli Roberto , Re Alessandro
Published: February 2023

Along with the fact that classical Hodgkin lymphoma (cHL) in older adults is frequently considered biologically different from cHL in younger patients, its most distinctive feature is its dismal clinical outcome due to the decreased effectiveness and greater toxicity of therapies. Although strategies to mitigate specific toxicities (e.g., cardiological and pulmonary) have obtained some results, in general, reduced-intensity schemes, proposed as an alternative to ABVD, have proved to be less effective. The addition of brentuximab vedotin (BV) to AVD, especially in a sequential scheme, has demonstrated good efficacy. However, the problem of toxicity persists even with this new therapeutic combination, with comorbidities remaining an important prognostic factor. The adequate stratification of functional status is necessary to distinguish between those patients who will benefit from full treatment and those who will benefit from alternative strategies. A simplified geriatric assessment based on the determination of ADL (activity of daily living), IADL (instrumental ADL), and CIRS-G (Cumulative Illness Rating Scale—Geriatric) scores is an easy-to-use tool that permits adequate patient stratification. Other factors of considerable impact on functional status such as sarcopenia and immunosenescence are currently being studied. A fitness-based treatment choice would also be very useful for relapsed or refractory patients, a more frequent and challenging situation than that is found in young cHL patients.
Bone Metastases and Health in Prostate Cancer: From Pathophysiology to Clinical Implications
Authors: Baldessari Cinzia , Pipitone Stefania , Molinaro Eleonora , Cerma Krisida , Fanelli Martina , Nasso Cecilia , Oltrecolli Marco , Pirola Marta , D’Agostino Elisa , Pugliese Giuseppe , Cerri Sara , Vitale Maria Giuseppa , Madeo Bruno , Dominici Massimo , Sabbatini Roberto
Published: February 2023

Clinically relevant bone metastases are a major cause of morbidity and mortality for prostate cancer patients. Distinct phenotypes are described: osteoblastic, the more common osteolytic and mixed. A molecular classification has been also proposed. Bone metastases start with the tropism of cancer cells to the bone through different multi-step tumor–host interactions, as described by the “metastatic cascade” model. Understanding these mechanisms, although far from being fully elucidated, could offer several potential targets for prevention and therapy. Moreover, the prognosis of patients is markedly influenced by skeletal-related events. They can be correlated not only with bone metastases, but also with “bad” bone health. There is a close correlation between osteoporosis—a skeletal disorder with decreased bone mass and qualitative alterations—and prostate cancer, in particular when treated with androgen deprivation therapy, a milestone in its treatment. Systemic treatments for prostate cancer, especially with the newest options, have improved the survival and quality of life of patients with respect to skeletal-related events; however, all patients should be evaluated for “bone health” and osteoporotic risk, both in the presence and in the absence of bone metastases. Treatment with bone-targeted therapies should be evaluated even in the absence of bone metastases, as described in special guidelines and according to a multidisciplinary evaluation.
Additional Primary Tumors Detected Incidentally on FDG PET/CT at Staging in Patients with First Diagnosis of NSCLC: Frequency, Impact on Patient Management and Survival
Authors: Kudura Ken , Ritz Nando , Templeton Arnoud J. , Kissling Marc , Kutzker Tim , Foerster Robert , Hoffmann Martin H. K. , Antwi Kwadwo , Kreissl Michael C.
Published: February 2023

We aimed to assess the frequency of additional primary malignancies detected incidentally on [18F]fluoro-D-glucose positron emission tomography/computed tomography (FDG-PET/CT) at staging in NSCLC patients. Moreover, their impact on patient management and survival was assessed. Consecutive NSCLC patients with available staging FDG-PET/CT between 2020 and 2021 were retrospectively enrolled. We reported whether further investigations of suspicious findings presumably not related to NSCLC were recommended and performed after FDG-PET/CT. Any additional imaging, surgery or multimodal management was considered as an impact on patient management. Patient survival was defined using overall survival OS and progression-free survival PFS. A total of 125 NSCLC patients were included, while 26 findings in 26 different patients were suspicious for an additional malignancy on FDG-PET/CT at staging. The most frequent anatomical site was the colon. A total of 54.2% of all additional suspicious lesions turned out to be malignant. Almost every malignant finding had an impact on patient management. No significant differences were found between NSCLC patients with suspicious findings versus no suspicious findings with regards to their survival. FDG-PET/CT performed for staging might be a valuable tool to identify additional primary tumors in NSCLC patients. Identification of additional primary tumors might have substantial implications for patient management. An early detection together with interdisciplinary patient management could prevent a worsening of survival compared to patients with NSCLC only.