bluuebunny/crossref_metadata_2025 huggingface dataset:
The potential of HBV cure: an overview of CRISPR-mediated HBV gene disruption
Authors: Yao Zhi Q. , Schank Madison B. , Zhao Juan , El Gazzar Mohamed , Wang Ling , Zhang Yi , Hill Addison C. , Banik Puja , Pyburn Jaeden S. , Moorman Jonathan P.
Published: October 2024

Hepatitis B virus (HBV) infection is a common cause of liver disease worldwide. The current antiviral treatment using nucleotide analogues (NAs) can only suppress de novo HBV replication but cannot eliminate chronic HBV infection due to the persistence of covalently closed circular (ccc) DNA that sustains viral replication. The CRISPR/Cas9 system is a novel genome-editing tool that enables precise gene disruption and inactivation. With high efficiency and simplicity, the CRISPR/Cas9 system has been utilized in multiple studies to disrupt the HBV genome specifically, eliciting varying anti-HBV effects both in vitro and in vivo. Additionally, multi-locus gene targeting has shown enhanced antiviral activity, paving the way for combination therapy to disrupt and inactivate HBV cccDNA as well as integrated HBV DNA. Despite its promising antiviral effects, this technology faces several challenges that need to be overcome before its clinical application, i.e., off-target effects and in vivo drug delivery. As such, there is a need for improvement in CRISPR/Cas9 efficiency, specificity, versatility, and delivery. Here, we critically review the recent literature describing the tools employed in designing guide RNAs (gRNAs) targeting HBV genomes, the vehicles used for expressing and delivering CRISPR/Cas9 components, the models used for evaluating CRISPR-mediated HBV gene disruption, the methods used for assessing antiviral and off-target effects induced by CRISPR/Cas9-mediated HBV gene disruption, and the prospects of future directions and challenges in leveraging this HBV gene-editing approach, to advance the HBV treatment toward a clinical cure.
The evolution of flexibility and function in the Fc domains of IgM, IgY, and IgE
Authors: Calvert Rosaleen A. , Nyamboya Rosemary A. , Beavil Andrew J. , Sutton Brian J.
Published: October 2024

Introduction

Antibody Fc regions harbour the binding sites for receptors that mediate effector functions following antigen engagement by the Fab regions. An extended “hinge” region in IgG allows flexibility between Fab and Fc, but in both the most primitive antibody, IgM, and in the evolutionarily more recent IgE, the hinge is replaced by an additional domain pair in the homodimeric six-domain Fc region. This permits additional flexibility within the Fc region, which has been exploited by nature to modulate antibody effector functions. Thus, in pentameric or hexameric IgM, the Fc regions appear to adopt a planar conformation in solution until antigen binding causes a conformational change and exposes the complement binding sites. In contrast, IgE-Fc principally adopts an acutely bent conformation in solution, but the binding of different receptors is controlled by the degree of bending, and there is allosteric communication between receptor binding sites.

Methods

We sought to trace the evolution of Fc conformational diversity from IgM to IgE via the intermediate avian IgY by studying the solution conformations of their Fc regions by small-angle X-ray scattering. We compared four extant proteins: human IgM-Fc homodimer, chicken IgY-Fc, platypus IgE-Fc, and human IgE-Fc. These are examples of proteins that first appeared in the jawed fish [425 million years ago (mya)], tetrapod (310 mya), monotreme (166 mya), and hominid (2.5 mya) clades, respectively.

Results and discussion

We analysed the scattering curves in terms of contributions from a pool of variously bent models chosen by a non-negative linear least-squares algorithm and found that the four proteins form a series in which the proportion of acutely bent material increases: IgM-Fc < IgY-Fc < plIgE-Fc < huIgE-Fc. This follows their order of appearance in evolution. For the huIgM-Fc homodimer, although none are acutely bent, and a significant fraction of the protein is sufficiently bent to expose the C1q-binding site, it predominantly adopts a fully extended conformation. In contrast, huIgE-Fc is found principally to be acutely bent, as expected from earlier studies. IgY-Fc, in this first structural analysis of the complete Fc region, exhibits an ensemble of conformations from acutely bent to fully extended, reflecting IgY’s position as an evolutionary intermediate between IgM and IgE.
A novel approach for rapidly determining the reproductive status of walleye pollock (Gadus chalcogrammus) using Raman spectroscopy
Authors: Neidetcher Sandra K. , Arrington Morgan B. , Helser Thomas E. , Goldstein Esther D. , Benson Irina M. , Waters Charles D.
Published: October 2024

Knowledge of the reproductive biology of fishes is essential for effective fisheries management. Information derived from an understanding of fish reproduction, such as size and age at maturity, is used in models to assess fish stocks and can affect estimates of important ecological processes such as recruitment, abundance, and trophic interactions. Common practices for determining the reproductive status of teleost fishes include macroscopic evaluation of gonads as well as histological analysis. However, macroscopic evaluation can be biased and histological analysis is time-consuming, resulting in limitations to spatial and temporal data availability. Here, we explore Raman spectroscopy of ovaries as a novel approach to rapidly determine the reproductive status of walleye pollock (Gadus chalcogrammus), a commercially and ecologically important species in the North Pacific. We used a two-stage partial least-squares (PLS) regression analysis followed by a linear discriminant analysis (LDA) to classify walleye pollock ovary samples as physiologically mature or immature and to subsequently predict their histologically-determined reproductive stage based on the Raman spectra. Biologically mature samples with visible yolk differentiated from mature and immature samples (non-yolked; 99% accuracy). Non-yolked ovaries that were physiologically mature (either mature non-developing or previously spawned) were further differentiated from physiologically immature ovaries (93% accuracy). In addition, detailed, histologically-determined reproductive stages of yolked samples also differentiated via Raman spectroscopy, but with reduced accuracy (79% - 86% accuracy). Our results indicate that accurate identification of maturity status and the reproductive staging of oocytes of walleye pollock based on spectral data from ovaries is possible. This can provide a fast and efficient way to increase the availability of a key component of reproductive data to inform fisheries research and management.
Grip strength and depressive symptoms in Chinese middle-aged and older adults: the mediating effects of cognitive function
Authors: Wang Xinzheng , Wu Lifei , Zhou Huifen , He Jiandong
Published: October 2024

Objective

This study investigates the associations and mediating pathways between grip strength, cognitive function, and depression in middle-aged and elderly individuals in China.

Methods

Utilizing data from the 2011 China Health and Retirement Longitudinal Study (CHARLS), we employed logistic regression and mediation analysis to examine the relationships and mediating factors between grip strength, cognitive function, and depression, while adjusting for potential confounders.

Results

The study included 6,841 participants, of whom 1,734 (25.35%) exhibited symptoms of depression. Our findings indicate that weak grip strength is significantly associated with an increased risk of depression (OR: 1.57, 95% CI: 1.32–1.87) among the middle-aged and elderly population. Conversely, good cognitive function was found to be protective against depression (OR: 0.94, 95% CI: 0.93–0.95). Grip strength indirectly affected depression through cognitive function, accounting for 9.4% of the total effect (β = −0.008, 95% CI [−0.013, −0.004]). This mediating effect was 23.8% in men (β = −0.013, 95% CI [−0.020, −0.007]); and 23.2% in those aged 60 years and over (β = −0.015, 95% CI [−0.022, −0.009]).

Conclusion

This study highlights that weak grip strength increases risk of depressive symptoms, and adequate cognitive function can mitigate the association between weak grip strength and an increased risk of depression among middle-aged and elderly individuals in China. Psychological care for elder adults with weak grip strength and poor cognitive function should be strengthened.
Transthyretin amyloidosis in patients with spinal stenosis who underwent spinal surgery: a systematic review and meta-analysis
Authors: Tamasauskas Domantas , Tamasauskiene Laura
Published: October 2024

Background

Accumulation of transthyretin amyloids (ATTR) is detected in ligamentum flavum in about 1/3 of patients underwent surgery for spinal stenosis. However, the significance of this finding is not known. The aim of this systematic review and meta-analysis is to analyze the incidence and importance of ATTR in patients with spinal stenosis who underwent spinal surgery.

Methods

The primary outcome measure was incidence of ATTR in patients with spinal stenosis. English language observational studies published within 10 years period were searched in Pubmed and Taylor and Francis databases.

Results

Nine articles were included in the systematic review. The incidence of positive ATTR among patients who underwent lumbar spinal surgery was 48% (95%CI 38–58%). ATTR deposits were found in the lumbar region the most frequently. Seven studies showed that patients with positive ATTR were older than those with negative. Five studies investigated and found a significant relationship between the ligamentum flavum thickness and positive ATTR. Five studies investigated cardiac involvement among patients with positive ATTR.

Conclusion

ATTR deposits are frequently found in older patients with spinal stenosis, especially in the lumbar region. The presence of ATTR deposits is related to ligamentum flavum thickness.
Natural resources used in the traditional medicine of the Marinaú community, Caxiuanã forest, Brazil
Authors: de Oliveira Paula Maria Correa , Sousa João Paulo Barreto , Albernaz Lorena C. , Salmen Espindola Laila , Coelho-Ferreira Márlia
Published: October 2024

Background

The diversity of Conservation Units in Brazil, ranging from Strict Protection areas like National Parks to Sustainable Use areas such as National Forests, supports the coexistence of human populations with extensive traditional knowledge of local natural resources. This traditional and local knowledge plays a crucial role in their subsistence and has significant potential to contribute to bioprospecting initiatives, as well as to enhance research and strategies for biological conservation. The São Sebastião de Marinaú community, situated within the Caxiuanã National Forest, offers valuable opportunities for ethnobiological studies.

Methods

The field study was carried out with 48 informants from the São Sebastião de Marinaú Community, Caxiuanã National Forest (CNF), Brazil, a Sustainable Use Conservation Unit. It involved participant observation, semi-structured interviews, and guided tours. Indices of Use Value (UV), Fidelity Level (FL), and Consensus Factor (ICF) indicated plant species with therapeutic potential.

Results

A total of 944 uses associated with 154 plants and 21 animals were reported. Statistical tests pointed out that factors such as gender and age are not determinants in the richness of known plants among the residents. The plants are distributed among 59 botanical families and 126 genera. They predominantly use species native to Brazil (69%), among which 47 are endemic to the Amazon. The medicines are prepared mainly by decoction of leaves and bark. According to UV, veronica (Dalbergia monetaria L.f.) was the most important. The animals used are all vertebrates, and paca (Cuniculus paca Linnaeus, 1766,) was the most cited. Bile and lard are the parts most used in the recipes. They mentioned 116 diseases, especially those of the digestive system. The ceruzeiro (Allantoma lineata (Mart. ex O.Berg) Miers) had a high consensus of local use, and no additional studies on this species exist.

Conclusion

This study underscores the vital role of traditional communities in sustainable conservation units, as their involvement is crucial for preserving plant and animal species essential to local traditional medicine. Such research also promotes the recognition of non-timber products as valuable raw materials with potential applications in the chemical and pharmaceutical industries. Additionally, mapping the occurrence and use of species in vulnerable conditions aids in developing effective conservation strategies for these resources.
Integrating basic human values with forest ecosystem services: pathways to sustainable forest management
Authors: Kobal Grum Darja , Bončina Andrej
Published: October 2024

The article explores the intricate relationship between basic human values and forest ecosystem services (FES). The study highlights the critical role that forests play in providing essential services for biodiversity, forest products, climate stabilization and human well-being, and emphasizes the need to understand and integrate human values into forest management and planning. Through a novel approach, this study explores how the concept of “forest” can elicit considerations of fundamental human values that diverge from conventional classifications and measurements of forest values. The study uses a comprehensive methodology, including surveys and content analysis, to uncover the hierarchical structure of human values associated with forests. This approach enables the identification of fundamental values that remain constant despite situational variations. The main results reveal a hierarchical structure of values, with Apollonian values being more prevalent than Dionysian ones. The study shows significant differences in the importance attributed to different FES, reflecting underlying value differences between residents. The study makes a novel contribution by systematically examining the links between human values and FES and proposing a profound and sustainable approach to forest management that takes into account the psychological dimensions of human-forest interactions. The study suggests that recognizing and incorporating the intrinsic human values into forest ecosystem service frameworks can improve sustainable forest management practices and ultimately foster a deeper connection between people and the forest environment.
Psychological wellbeing among carers of children and adolescents with physical disabilities in North-Eastern Tanzania—a cross-sectional survey study
Authors: Swai Elia Asanterabi , Moshi Haleluya Imanueli , Msuya Sia Emmanueli , Sörlin Ann , Sahlen Klas-Göran , Lindkvist Marie
Published: October 2024

Introduction

Caring for physically disabled children, particularly in contexts where resources are often insufficient or absent, exposes primary carers to significant psychological strain. The lack of structured mechanisms to address this psychological burden poses a considerable threat to the wellbeing of both carers and the children with disabilities under their care. However, research on the psychological wellbeing of carers in Tanzania is scarce. This study aimed to evaluate the psychological wellbeing and symptoms of anxiety and depression, along with associated factors, of carers of children, and adolescents with physical disabilities in the Kilimanjaro region.

Methods

A cross-sectional survey was conducted from November 2020 to June 2021 in the Kilimanjaro region, involving 212 carers. The Swahili versions of the WHO-5 Wellbeing Index and Hopkins Symptoms Check List-25 were used to assess psychological wellbeing and symptoms of anxiety and depression. Data were analysed using IBM SPSS Statistics V.28. Multivariable linear and binary logistic regression were used to assess the associations.

Results

More than four fifths (81%) of the carers were parents, and most of these parents were mothers (92%). Of the 212 carers, more than half (51%) exhibited poor psychological wellbeing, 42% had symptoms of anxiety, and 38% symptoms of depression. Notably, poor psychological wellbeing was significantly associated with symptoms of anxiety and depression. Results of a multivariable regression analysis indicated that extended family support was associated with better psychological wellbeing and lower odds of symptoms of anxiety and depression. A higher estimated monthly income was associated with better psychological wellbeing, and lower odds of symptoms of depression. Access to rehabilitation for the child was associated with improved psychological wellbeing and decreased likelihood of symptoms of anxiety.

Conclusion

Many carers of children, and adolescents with physical disabilities in Tanzania are at high risk of having poor psychological wellbeing, and symptoms of anxiety and depression. Support from extended-family networks is crucial in promoting good psychological wellbeing. The government in Tanzania should improve rehabilitation services, especially in disadvantaged rural areas, and integrate mental-health screening for carers in both rehabilitation programmes and primary healthcare.
G-quadruplex DNA and RNA in cellular senescence
Authors: Diaz Escarcega Rocio , Marshall Paul , Tsvetkov Andrey S.
Published: October 2024

Normal cells divide, are damaged, and are repaired across their lifetime. As cells age, they enter cellular senescence, characterized by a permanent state of cell-cycle arrest triggered by various stressors. The molecular mechanisms that regulate senescent phenotypes have been actively investigated over the last several decades; however, one area that has been neglected is how G-quadruplex (G4) DNA and RNA (G4-DNA and G4-RNA) mediate senescence. These non-canonical four-stranded DNA and RNA structures regulate most normative DNA and RNA-dependent processes, such as transcription, replication, and translation, as well as pathogenic mechanisms, including genomic instability and abnormal stress granule function. This review also highlights the contribution of G4s to sex differences in age-associated diseases and emphasizes potential translational approaches to target senescence and anti-aging mechanisms through G4 manipulation.
A comprehensive survey of space robotic manipulators for on-orbit servicing
Authors: Alizadeh Mohammad , Zhu Zheng H.
Published: October 2024

On-Orbit Servicing (OOS) robots are transforming space exploration by enabling vital maintenance and repair of spacecraft directly in space. However, achieving precise and safe manipulation in microgravity necessitates overcoming significant challenges. This survey delves into four crucial areas essential for successful OOS manipulation: object state estimation, motion planning, and feedback control. Techniques from traditional vision to advanced X-ray and neural network methods are explored for object state estimation. Strategies for fuel-optimized trajectories, docking maneuvers, and collision avoidance are examined in motion planning. The survey also explores control methods for various scenarios, including cooperative manipulation and handling uncertainties, in feedback control. Additionally, this survey examines how Machine learning techniques can further propel OOS robots towards more complex and delicate tasks in space.
Soil health – a perspective
Authors: Fausak Lewis K. , Bridson Noa , Diaz-Osorio Fernanda , Jassal Rachhpal S. , Lavkulich Les M.
Published: October 2024

Governments and organizations are expressing growing concerns about soil health, driven largely by uncertainties of food security with an increasing human population and unpredictable effects of climate change. Although considerable literature and debate exist, there is discord around the question, what is a healthy soil? This is not surprising, given the complex roles the soil provides, from the range of food, fiber and medical products, hosting a biodiverse community, and supporting the water and nutrient cycles. While a consensus seems to suggest that a soil in good health should be able to provide goods and services in perpetuity, this does not define soil health, rather its provisioning functions. To explore the question, ‘what is healthy?’, we propose an analogy comparing indicators of human and soil health. For example, to identify the cause of a symptom, we compare the diagnostic pH in both humans and soil, demonstrating the similarities between the way human and soil health concerns are addressed. Additionally, we consider the context that necessitates health and use a set of holistic predictors to link human and soil health further. In humans, genetics express many traits and can predispose one to certain illnesses or diseases, in the same way, parent material, soil texture, and length of time exposed to weathering can inform a soil’s capability and predisposition for certain habitats or uses. In both cases, science informs the state of health and appropriate management solutions. We posit the null hypothesis “the concept of human health cannot be applied to soil”.
Potential of integrated multitrophic aquaculture to make prawn farming sustainable in Bangladesh
Authors: Alam Md. Mehedi , Jørgensen Niels O. G. , Bass David , Santi Morena , Nielsen Max , Rahman Md. Asadur , Hasan Neaz A. , Bablee Alif Layla , Bashar Abul , Hossain Md. Ismail , Hansen Lars Hestbjerg , Haque Mohammad Mahfujul
Published: October 2024

Farmed freshwater prawn (Macrobrachium rosenbergii) and black tiger shrimp (Penaeus monodon) comprise a significant portion of Bangladesh’s seafood exports, raising concerns about their environmental impacts. Freshwater prawn farms, which require a relatively high amount of feed supply, release 1.0 MT CO2-equivalents/year, equating to 18.8 kg CO2e/MT prawn, contributing significantly to global warming and climate change risks. Integrated Multi-Trophic Aquaculture (IMTA) offers an alternative farming method to conventional prawn farming systems, as it minimizes greenhouse gas (GHG) emissions and climate change impacts. Systematically reviewing 112 scientific articles on IMTA, this article offers recommendations for adopting IMTA to promote sustainable freshwater prawn farming in Bangladesh. IMTA is undergoing extensive experimentation and practice in many parts of the world, offering economic benefits, social acceptability, and environmental sustainability. In addition to native prawn species, various indigenous organic extractive freshwater mollusks, and inorganic extractive plants are available which can seamlessly be used to tailor the IMTA system. Extractive organisms, including aquatic mollusks and plants within prawn farms, can capture blue carbon effectively lowering GHG emissions and helping mitigate climate change impacts. Aquatic mollusks offer feed for fish and livestock, while aquatic plants serve as a dual food source and contribute to compost manure production for crop fields. Research on IMTA in Bangladesh was primarily experimented on finfish in freshwater ponds, with the absence of studies on IMTA in prawn farms. This necessitates conducting research at the prawn farmer level to understand the production of extractive aquatic mollusk and plants alongside prawn in the prawn-producing regions of southwestern Bangladesh.
Application of propionate-producing bacterial consortium in ruminal methanogenesis inhibited environment with bromoethanesulfonate as a methanogen direct inhibitor
Authors: Jeong Jongsik , Yu Chaemin , Kang Ryukseok , Kim Myunghoo , Park Tansol
Published: October 2024

Methane production in ruminants is primarily due to the conversion of metabolic hydrogen (H2), produced during anaerobic microbial fermentation, into methane by ruminal methanogens. While this process plays a crucial role in efficiently disposes of H2, it also contributes to environmental pollution and eliminating methane production in the rumen has proven to be challenging. This study investigates the use of probiotics, specifically propionate-producing bacteria, to redirect accumulated H2 in a methane-mitigated environment. For this objective, we supplemented experimental groups with Lactiplantibacillus plantarum and Megasphaera elsdenii for the reinforced acrylate pathway (RA) and Selenomonas ruminantium and Acidipropionibacterium thoenii for the reinforced succinate pathway (RS), as well as a consortium of all four strains (CB), with the total microbial concentration at 1.0 × 1010 cells/mL. To create a methane-mitigated environment, 2-bromoethanesulfonate (BES) was added to all experimental groups at a dose of 15 mg/0.5 g of feed. BES reduced methane production by 85% in vitro, and the addition of propionate-producing bacteria with BES further decreased methane emission by up to 94% compared with the control (CON) group. Although BES did not affect the alpha diversity of the ruminal bacteriome, it reduced total volatile fatty acid production and altered beta diversity of ruminal bacteriota, indicating microbial metabolic adaptations to H2 accumulation. Despite using different bacterial strains targeting divergent metabolic pathways (RA and RS), a decrease in the dominance of the [Eubacterium] ruminantium group suggesting that both approaches may have a similar modulatory effect. An increase in the relative abundance of Succiniclasticum in the CB group suggests that propionate metabolism is enhanced by the addition of a propionate-producing bacterial consortium. These findings recommend using a consortium of propionate-producing bacteria to manage H2 accumulation by altering the rumen bacteriome, thus mitigating the negative effects of methane reduction strategies.
Global A-to-I RNA editing during myogenic differentiation of goat MuSCs
Authors: Xu Xiaoli , Zhang Mancheng , Zhan Siyuan , Chen Yuan , Wei Chengqi , Cao Jiaxue , Guo Jiazhong , Dai Dinghui , Wang Linjie , Zhong Tao , Zhang Hongping , Li Li
Published: October 2024

Background

RNA editing, especially A-to-I editing sites, is a common RNA modification critical for stem cell differentiation, muscle development, and disease occurrence. Unveiling comprehensive RNA A-to-I editing events associated with myogenesis of the skeletal muscle satellite cells (MuSCs) is essential for extending our knowledge of the mechanism underpinning muscle development.

Results

A total of 9,632 RNA editing sites (RESs) were screened in the myoblasts (GM), myocytes (DM1), and myotubes (DM5) samples. Among these sites, 4,559 A-to-I edits were classified and further analyzed. There were 3,266 A-to-I sites in the protein-coding region, out of which 113 missense sites recoded protein. Notably, five A-to-I sites in the 3′ UTR of four genes (TRAF6, NALF1, SLC38A1, ENSCHIG00000019092) altered their targeted miRNAs. Furthermore, a total of 370 A-to-I sites with different editing levels were detected, including FBN1, MYH10, GSK3B, CSNK1D, and PRKACB genes. These genes were predominantly enriched in the cytoskeleton in muscle cells, the hippo signaling pathway, and the tight junction. Furthermore, we identified 14 hub genes (TUFM, GSK3B, JAK2, RPSA, YARS1, CDH2, PRKACB, RUNX1, NOTCH2, CDC23, VCP, FBN1, RARS1, MEF2C) that potentially related to muscle development. Additionally, 123 stage-specific A-to-I editing sites were identified, with 43 sites in GM, 25 in DM1, and 55 in DM5 samples. These stage-specific edited genes significantly enriched essential biological pathways, including the cell cycle, oocyte meiosis, motor proteins, and hedgehog signaling pathway.

Conclusion

We systematically identified the RNA editing events in proliferating and differentiating goat MuSCs, which was crucial for expanding our understanding of the regulatory mechanisms of muscle development.
Identification of multiple novel viruses in bar-headed goose feces from Tibet of China
Authors: Sun Yijie , Wang Yan , Ji Li , Pei Yifei , Sun Xiaoyi , Ji Likai , Shen Quan , Wang Xiaochun , Liu Yuwei , Yang Shixing , Zhang Wen
Published: October 2024

Introduction

The bar-headed goose is a typical high-altitude bird that primarily inhabits alpine lakes and wetlands in Central Asia, with a remarkable ability to adapt to high elevations. Previous studies have shown that they can be infected with parasites such as Cryptosporidium spp. At present, there were few reports on its infection with the virus.

Methods

In this study, we utilized viral metagenomics to conduct a detailed analysis of the viral components in the fecal samples of bar-headed geese (Anser indicus) from the Tibet region of China.

Results

Multiple novel viruses were identified including four novel astroviruses, four novel caliciviruses, ten novel circoviruses, and nineteen novel parvoviruses. Among them, four astroviruses shared the highest amino acid sequence identities of 63.45–99.47% with different avastrovirus strains. Four caliciviruses and ten circoviruses were identified as unclassified caliciviruses and unclassified circoviruses, separately. Nineteen parvoviruses clustering into four groups maybe four different novel species of the genus Chaphamaparvovirus.

Conclusion

These newly discovered viruses have potential implications for the health of avian species, particularly bar-headed geese. This study not only helps us understand the health status of bar-headed geese, but also offers crucial genomic information for future disease prevention and treatment strategies.
Analysis and Experimental Study for Fatigue Performance of Wing-Fuselage Connection Structure for Unmanned Aerial Vehicle
Authors: Sui Lijun , Sun Youchao , Kang Min
Published: October 2024

(1) Background: As the principal structural element of an unmanned aerial vehicle (UAV), a lightweight, high-performance wing-fuselage connection structure plays an important role in UAV structural integrity. Previous studies focused on the static strength characteristics of aluminum and high-strength steel wing-fuselage connection structures. With the widespread usage of titanium material in aviation, research should address the fatigue performance of titanium wing-fuselage connection structures. This paper aims to investigate the fatigue performance of the titanium wing-fuselage connection structure by using analysis and experimental methods, and to develop a reliable fatigue assessment method based on the experimental results. (2) Methods: General and detail finite element models were established, and the stress distribution at the detail fatigue design point was obtained via finite element analysis. Based on the equivalent life curve theory, a dimensionless value, detail fatigue value (DF), was proposed to characterize the fatigue performance for structure. By using detail fatigue value (DF) method, the theoretical fatigue performance value was calculated. The fatigue test of the wing-fuselage connection structure was conducted, and the fatigue life was determined according to the test results. (3) Results: For the wing joint with fatigue failure in the test, the test fatigue performance was close to the theoretical value. It can be concluded from the DF value that the analysis and test results are consistent, and the values obtained from the analysis are more conservative than test results. (4) Conclusions: The error between the test DF value and the theoretical analysis DF value is small, and the theoretical analysis of fatigue performance can represent the fatigue characteristics of the wing-fuselage connection structure. The detail fatigue value (DF) method can predict the fatigue performance of the structure quite well.
High-Fidelity Simulations of Flight Dynamics and Trajectory of a Parachute–Payload System Leaving the C-17 Aircraft
Authors: Ghoreyshi Mehdi , Bergeron Keith , Seidel Jürgen
Published: October 2024

This article examines the flight dynamics and trajectory analysis of a parachute–payload system deployed from a C-17 aircraft. The aircraft is modeled with an open cargo door, extended flaps, and four turbo-fan engines operating at an altitude of 2000 feet Above Ground Level (AGL) and an airspeed of 150 knots. The payloads consist of simplified CONEX containers measuring either 192 inches or 240 inches in length, 9 feet in width, and 5.3 feet in height, with their mass and moments of inertia specified. At positive deck angles, gravitational forces cause these payloads to begin a gradual descent from the rear of the aircraft. For aircraft at zero deck angle, a ring-slot parachute with approximately 20% geometric porosity is utilized to extract the payload from the aircraft. This study specifically employs the CREATE-AV Kestrel simulation software to model the chute-payload system. The extraction and suspension lines are represented using Kestrel’s Catenary capability, with the extraction line connected to the floating confluence points of the CONEX container and the chute. The chute and payload will experience coupled motion, allowing for an in-depth analysis of the flight dynamics and trajectory of both elements. The trajectory data obtained will be compared to that of a payload (without chute and cables) exiting the aircraft at positive deck angles. An adaptive mesh refinement technique is applied to accurately capture the engine exhaust flow and the wake generated by the C-17, chute, and payloads. Friction and ejector forces are estimated to align the exit velocity and timing with those recorded during flight testing. The results indicate that the simulation of extracted payloads aligns with expected trends observed in flight tests. Notably, higher deck angles result in longer distances from the ramp, leading to increased exit velocities and reduced payload rotation rates. All payloads exhibit clockwise rotation upon leaving the ramp. The parachute extraction method yields significantly higher exit velocities and shorter exit times, while the payload-chute acceleration correlates with the predicted drag of the chute as demonstrated in prior studies.
Evaluating Reduced-Order Urban Wind Models for Simulating Flight Dynamics of Advanced Aerial Mobility Aircraft
Authors: Krawczyk Zack , Vuppala Rohit K. S. S. , Paul Ryan , Kara Kursat
Published: October 2024

Advanced Aerial Mobility (AAM) platforms are poised to begin high-density operations in urban areas nationwide. This new category of aviation platforms spans a broad range of sizes, from small package delivery drones to passenger-carrying vehicles. Unlike traditional aircraft, AAM vehicles operate within the urban boundary layer, where large structures, such as buildings, interrupt the flow. This study examines the response of a package delivery drone, a general aviation aircraft, and a passenger-carrying urban air mobility aircraft through an urban wind field generated using Large Eddy Simulations (LES). Since it is burdensome to simulate flight dynamics in real-time using the full-order solution, reduced-order wind models are created. Comparing trajectories for each aircraft platform using full-order or reduced-order solutions reveals little difference; reduced-order wind representations appear sufficient to replicate trajectories as long as the spatiotemporal wind field is represented. However, examining control usage statistics and time histories creates a stark difference between the wind fields, especially for the lower wing-loading package delivery drone where control saturation was encountered. The control saturation occurrences were inconsistent across the full-order and reduced-order winds, advising caution when using reduced-order models for lightly wing-loaded aircraft. The results presented demonstrate the effectiveness of using a simulation environment to evaluate reduced-order models by directly comparing their trajectories and control activity metrics with the full-order model. This evaluation provides designers valuable insights for making informed decisions for disturbance rejection systems. Additionally, the results indicate that using Reynolds-averaged Navier–Stokes (RANS) solutions to represent urban wind fields is inappropriate. It was observed that the mean wind field trajectories fall outside the 95% confidence intervals, a finding consistent with the authors’ previous research.
Research on Chip Removal Performance of Rotary Percussive Ultrasonic Drill for Planetary Rock Sampling
Authors: Wang Yinchao , Zheng Dongdong , Yin Zihao , Zhang Weiwei , Zu Lin , Tao Guanghong , Yu Suyang
Published: October 2024

The objective of the kinematic model of rock chips is to examine the impact of the driving parameters of drilling tools on the effective chip removal speed, with a view to analyze the chip removal performance of rotary percussion ultrasonic drilling. The discrete element numerical simulation method, EDEM-2022 simulation software, was employed to establish a simulation model, match the simulation parameters, and conduct a simulation study of chip removal under the action of rotary percussion. This was performed to analyze the influence of the driving parameters on the chip conveying process. In order to ascertain the order of parameters affecting the degree of conveyance rate as well as the optimal driving parameters, orthogonal experiments were designed based on Taguchi’s experimental analysis method.
Assessing the Corrosive Effects of Unmelted Particles in Additively Manufactured Ti6Al4V: A Study in Simulated Body Fluid
Authors: Pal Surinder , Velay Xavier , Saleem Waqas
Published: October 2024

This study investigates the corrosion behavior of Grade 23 Ti6Al4V alloys produced through laser powder bed fusion (L-PBF) when exposed to simulated body fluid at room temperature, focusing on the role of unmelted particles. This research aims to understand how these microstructural features, resulting from the additive manufacturing process, influence the corrosion resistance of the alloys. It was observed that unmelted particles serve as critical sites for initiating localized corrosion, including pitting, which significantly compromises the material’s overall durability. Electrochemical testing and detailed surface analysis revealed that these particles, alongside other defects such as voids, exacerbate the susceptibility to corrosion in biomedical environments where high material reliability is paramount. Weight loss measurements conducted over exposure periods of 48 h, 96 h, and 144 h demonstrated a progressive increase in corrosion, correlating with the presence of unmelted particles. These findings underscore the importance of optimizing L-PBF processing parameters to minimize the formation of unmelted particles, thereby enhancing corrosion resistance and extending the operational lifespan of Ti6Al4V implants in biomedical applications.
Renal Sugar Metabolites and mRNA Expression of Glucose Transporters in Meat-Type Chickens with Differing Residual Water Intake
Authors: Milfort Marie C. , Ghareeb Ahmed F. A. , Ariyo Oluwatomide W. , Kwakye Josephine , Hartono Evan , Sovi Selorm , Aryal Bikash , Fuller Alberta L. , El Sabry Mohamed I. , Stino Farid , Rekaya Romdhane , Aggrey Samuel E.
Published: October 2024

Molecular differences exist between birds with high residual water intake (HRWI) compared to those with low residual water intake (LRWI). Residual water intake (RWI) is defined as the difference between the water intake of a bird and the expected water intake corrected for metabolic body weight, feed intake, and body weight gain. Tissue metabolomic analysis revealed significantly increased kidney glucose, fructose, and arabitol in the LRWI group compared to the HRWI group. mRNA expression analysis of apical sodium glucose cotransporters SGLT1, SGLT4, SGLT5, and SGLT6 showed decreased expression of SGLTs 1, 5, and 6 in LRWI birds (p < 0.05), whereas SGLT4 expression was increased compared with HRWI birds (p < 0.01). An analysis of basal glucose transporters GLUT1, GLUT2, GLUT5, and GLUT9 showed significantly increased GLUT2 expression in LRWI birds compared with HRWI birds (p < 0.01). We postulate that SGLT4 is the main apical transporter in chicken kidneys and that its increased expression reduces these birds’ need for water, resulting in less drinking. This is balanced by the increased expression of the basal transporter GLUT2, indicating better glucose retention, which may partly explain the physiological mechanism behind why these birds drink less water. Innately driven broiler water intake could therefore be influenced by the expression of kidney solute transporters.
Correction: Correia et al. Beyond Penicillin: The Potential of Filamentous Fungi for Drug Discovery in the Age of Antibiotic Resistance. 2023, 12, 1250
Authors: Correia João , Borges Anabela , Simões Manuel , Simões Lúcia C.
Published: October 2024

Manuel Simões was included as a corresponding author in the original publication [...]
Lower Energy-Demanding Extraction of Bioactive Triterpene Acids by Microwave as the First Step towards Biorefining Residual Olive Skin
Authors: Gómez-Cruz Irene , Contreras María del Mar , Romero Inmaculada , Castro Eulogio
Published: October 2024

In the olive oil industry, a pit fraction is collected from olive pomace and split into a clean pit fraction and a residual olive skin-rich fraction, which does not an industrial application. Therefore, in this work, microwave-assisted extraction (MAE) was applied to obtain high-value triterpene acids (maslinic acid and oleanolic acid) from this biomass using the renewable solvent ethanol. The response surface methodology was used to gain a deeper understanding of how the solvent (ethanol–water, 50–100% v/v), time (4–30 min), and temperature (50–120 °C) affect the extraction performance, as well as the energy required for the process. The effect of milling was also studied and the solid-to-liquid ratio was also evaluated, and overall, a good compromise was found at 10% (w/v) using the raw sample (unmilled biomass). The optimised conditions were applied to residual olive skin sourced from various industries, yielding up to 5.1 g/100 g and 2.2 g/100 g dry biomass for maslinic acid and oleanolic acid, respectively. In conclusion, the residual olive skin is a promising natural source of these triterpene acids, which can be extracted using MAE, releasing extracted solids rich in polymeric carbohydrates and lignin that can be valorised under a holistic biorefinery process.
Influence of Acetylene Concentration on N2O and N2 Emissions from an Intensive Vegetable Soil under Anoxic and Oxic Conditions
Authors: Cao Wenchao , Zhang Runzhi , Li Yanqing , Pan Haoqin , Zhao Fei , Wang Cuicui , Xin Shuo , Li Dong , Gao Ziyu , Wang Yajing
Published: October 2024

Acetylene (C2H2) is often employed to assess soil total denitrification (N2O + N2) due to its ease of implementation. However, this technique underestimates soil denitrification in soils with low nutrient contents, particularly those supporting grain yields. To our knowledge, there are limited studies that have specifically investigated the impact of C2H2 on nutrient-rich vegetable soils, especially concerning the emissions of N2 and N2O and the nitrogenous gas product ratio (i.e., N2O/(N2O + N2)). In this study, we conducted both anoxic and oxic incubations at various C2H2 concentrations (0%, 0.01%, and 10%, v/v) and utilized a robotized sampling and analysis system to quantify soil N2, N2O, and CO2 emissions. Our findings revealed that the cumulative N2O production in soil treated with 10%C2H2 was significantly lower than that in soil treated with 0.01%C2H2 and soil without C2H2. Contrarily, high concentrations of C2H2 (10%, v/v) led to increased N2 production. Similar trends were observed under oxic conditions, where 10%C2H2 concentration did not enhance N2O production but markedly increased N2 and CO2 emissions. Moreover, the N2O/(N2O + N2) product ratio was notably higher in soils treated with 0%C2H2 compared to the 10%C2H2 treatment under anoxic conditions. These findings indicate that high concentrations of acetylene could facilitate the reduction of N2O to N2 and lead to underestimated soil total denitrification in vegetable soil, regardless of anoxic or oxic conditions. This discovery underscores the drawbacks when employing high concentrations of acetylene to evaluate actual total denitrification in intensive greenhouse vegetable soils, highlighting the necessity for further investigation into alternative methodologies.
The Diagnostic Value of bpMRI in Prostate Cancer: Benefits and Limitations Compared to mpMRI
Authors: Iacob Roxana , Manolescu Diana , Stoicescu Emil Robert , Cerbu Simona , Bardan Răzvan , Ghenciu Laura Andreea , Cumpănaș Alin
Published: October 2024

Prostate cancer is the second most common cancer in men and a leading cause of death worldwide. Early detection is vital, as it often presents with vague symptoms such as nocturia and poor urinary stream. Diagnostic tools like PSA tests, ultrasound, PET-CT, and mpMRI are essential for prostate cancer management. The PI-RADS system helps assess malignancy risk based on imaging. While mpMRI, which includes T1, T2, DWI, and dynamic contrast-enhanced imaging (DCE), is the standard, bpMRI offers a contrast-free alternative using only T2 and DWI. This reduces costs, acquisition time, and the risk of contrast-related side effects but has limitations in detecting higher-risk PI-RADS 3 and 4 lesions. This study compared bpMRI’s diagnostic accuracy to mpMRI, focusing on prostate volume and PI-RADS scoring. Both methods showed strong inter-rater agreement for prostate volume (ICC 0.9963), confirming bpMRI’s reliability in this aspect. However, mpMRI detected more complex conditions, such as periprostatic fat infiltration and iliac lymphadenopathy, which bpMRI missed. While bpMRI offers advantages like reduced cost and no contrast use, it is less effective for higher-risk lesions, making mpMRI more comprehensive.
Non-Invasive Muscle Metabolism Assessment with Near-Infrared Spectroscopy and Electrical Muscle Stimulation
Authors: Tanaka Riki , Shigemori Yutaka , Moriguchi Tetsushi
Published: October 2024

Background: Muscle biopsy, the gold standard for assessing muscle aerobic capacity, is an invasive procedure. Noninvasive alternatives, such as 31-phosphorus magnetic resonance spectroscopy (31P-MRS) and near-infrared spectroscopy (NIRS), provide valuable insights, with NIRS providing superior time resolution and ease of use compared with 31P-MRS. Objectives: This study aimed to evaluate muscle metabolism using a 6 s muscle contraction phase with electrical muscle stimulation (EMS) and to assess the impact of EMS on exercise performance under hyperbaric conditions with elevated oxygen pressure. Methods: This study included nine male participants (21 ± 2 years) who underwent 5 min of EMS on the forearm flexor muscle group, with muscle oxygen dynamics assessed using NIRS. For statistical analysis, the mean values between groups were assessed using paired t-tests, and associations were evaluated using Pearson’s correlation coefficient. Results: Spontaneous blood flow interruption during EMS-induced muscle activity indicated the potential for assessing muscle metabolism without disrupting external blood flow. A significant negative correlation was observed between oxygen consumption and changes in oxygenated hemoglobin levels during muscle activity under hyperbaric conditions. Conclusions: This study demonstrates that skeletal muscle metabolism can be measured using a brief 6 s quantitative EMS. Furthermore, hyperbaric exercise appears to enhance aerobic capacity by increasing the rate and availability of oxygen consumption during exercise.