bluuebunny/crossref_metadata_2025 huggingface dataset:
Acetate Downregulates the Activation of NLRP3 Inflammasomes and Attenuates Lung Injury in Neonatal Mice With Bronchopulmonary Dysplasia
Authors: Zhang Qian , Ran Xiao , He Yu , Ai Qing , Shi Yuan
Published: February 2021

Background: Bronchopulmonary dysplasia (BPD) is a common pulmonary complication in preterm infants. Acetate is a metabolite produced by the gut microbiota, and its anti-inflammatory function is well known. The role of acetate in BPD has not been studied. Here, we investigate the effects of acetate on lung inflammation and damage in mice model of BPD.

Objective: To investigate the role of acetate in the development of BPD.

Methods: C57BL/6 mice were randomly divided into three groups on the 3rd day after birth: room air group, hyperoxia group, and hyperoxia + acetate (250 mM, 0.02 ml/g) group. The expression of inflammatory factors was determined by ELISA and RT-PCR, and NLRP3 and caspase-1 were detected by Western blot. High-throughput sequencing was used to detect bacterial communities in the mice intestines.

Results: After acetate treatment, the expression levels of TNF-α, IL-1β, IL-18, NLRP3, and caspase-1 were significantly reduced, while the expression of GPR43 was increased. In the BPD mice treated with acetate, the proportion of Escherichia-Shigella was lower than in placebo-treated BPD mice, while the abundance of Ruminococcus was increased.

Conclusions: These results indicate that acetate may regulate intestinal flora and reduce inflammatory reactions and lung injury in BPD. Therefore, acetate may be an effective drug to protect against neonatal BPD.
Overexpression of DBF-Interactor Protein 6 Containing an R3H Domain Enhances Drought Tolerance in Populus L. (Populus tomentosa)
Authors: Liu Yang , Yao Xinzhuan , Zhang Lu , Lu Litang , Liu Renxiang
Published: February 2021

Drought is the primary disaster that endangers agricultural production, including animal husbandry, and affects the distribution, growth, yield, and quality of crops. Previous study had revealed that DIP, as a potential regulator of DBF activity, played an important role in response to drought stress in maize. In this study, a total of 67 DIPs were identified from seventeen land plants, including six tobacco DIPs (NtDIPs). NtDIP6 gene was further selected as a candidate gene for subsequent experiments based on the phylogenetic analysis and structural analysis. The transgenic tobacco and poplar plants over-expressing NtDIP6 gene were generated using the Agrobacterium- mediated method. Although there was not phenotypic difference between transgenic plants and wild-type plants under normal conditions, overexpression of the NtDIP6 gene in transgenic tobacco and poplar plants enhanced the drought tolerance under drought treatments in comparison with the wild type. The content of antioxidant defense enzymes peroxidase (POD), catalase (CAT), and the photosynthetic rate increased in NtDIP6-Ox transgenic tobacco and poplar plants, while the content of malondialdehyde decreased, suggesting that the overexpression of NtDIP6 enhances the antioxidant capacity of transgenic poplar. Furthermore, the results of qRT-PCR showed that the level of expression of drought-related response genes significantly increased in the NtDIP6-Ox transgenic plants. These results indicated that NtDIP6, as a positive response regulator, improves drought stress tolerance by scavenging superoxide via the accumulation of antioxidant defense enzymes.
The Relationship Between Family Support and e-Learning Engagement in College Students: The Mediating Role of e-Learning Normative Consciousness and Behaviors and Self-Efficacy
Authors: Gao Hong , Ou Yangli , Zhang Zhiyuan , Ni Menghui , Zhou Xinlian , Liao Li
Published: February 2021

Due to the current COVID-19 pandemic, colleges and universities have implemented network teaching. E-learning engagement is the most important concern of educators and parents because this will directly affect student academic performance. Hence, this study focuses on students’ perceived family support and their e-learning engagement and analyzes the effects of e-learning normative consciousness and behaviors and self-efficacy on the relationship between family support and e-learning engagement in college students. Prior to this study, the relationship between these variables was unknown. Four structural equation models revealed the multiple mediating roles of e-learning normative consciousness and behaviors and self-efficacy in the relationship between family support and e-learning engagement. A total of 1,317 college students (mean age=19.51; 52.2% freshman) voluntarily participated in our study. The results showed that e-learning normative consciousness and behaviors and self-efficacy played significant and mediating roles between students’ perceived family support and e-learning engagement. Specifically, these two individual variables fully mediated the relationship between students’ perceived family support and e-learning engagement. The multiple mediation model showed that family members can increase family support of their children by creating a household environment conducive to learning, displaying positive emotions, demonstrating the capability to assist their children, advocating the significance of learning normative consciousness and behaviors, and encouraging dedicated and efficient learning. The findings complement and extend the understanding of factors influencing student e-learning engagement.
Supporting Holistic Wellbeing for Performing Artists During the COVID-19 Pandemic and Recovery: Study Protocol
Authors: Stuckey Melanie , Richard Véronique , Decker Adam , Aubertin Patrice , Kriellaars Dean
Published: February 2021

The COVID-19 pandemic resulted in the abrupt closure of circus schools, venues, and companies, introducing a myriad of novel stressors. Performers and students must now attempt to maintain their technical, physical, artistic, creative, and cognitive abilities without in-person support from their coaches and must manage the isolation from their training and performing spaces. For circus artists, the transposition of the work space to a home environment is not possible, which creates novel stressors that could lead to the exacerbation and escalation of mental health issues. The purpose of this study is to develop, implement and evaluate a holistic interventional program based on the socio-ecological model of resilience and operationalized through physical literacy. This will be a prospective longitudinal study with a retrospective comparison to data from a similar student cohort pre-pandemic. Interventions were designed using a population-specific, participant-based developmental model within a knowledge translation framework. The interventional program includes group webinars, small group information sessions, and one-on-one Zoom meetings, in addition to the distribution of electronic educational materials. The interventions will holistically provide psychological, physical, social, technical, artistic, and creative supports. Resources will be deployed throughout the closure period and through recovery, as transitions to return to training after prolonged hiatus will magnify known psychological and physical difficulties. Repeated, longitudinal assessment of students will be utilized to track changes over time at key transitions in the pandemic and school year and will be compared to a pre-pandemic school year. The framework for this program will be translatable to other performing arts and high-performance contexts. The program has implications for the mental health and overall wellbeing of artists and for cultural and economic recovery of the industry.
Making Art Therapy Virtual: Integrating Virtual Reality Into Art Therapy With Adolescents
Authors: Shamri Zeevi Liat
Published: February 2021

In recent years, the field of art therapy has sought to adapt traditional treatment approaches to today’s innovative technological environments when working with adolescent “digital natives.” In their clinic, art therapists often struggle with lack of cooperation when treating adolescents during sessions. This article presents two case studies that explore how Virtual Reality (VR) technology can be combined with traditional art therapy to treat adolescents suffering from anxiety and social difficulties. It is suggested that this type of technology may lead to a better understanding of the needs of adolescents by adopting their vantage point and hence better outcomes.
Students’ Perception of Teachers’ Reference Norm Orientation and Cheating in the Classroom
Authors: Marksteiner Tamara , Nishen Anna K. , Dickhäuser Oliver
Published: February 2021

Students’ cheating is a serious problem: It undermines the chance to adequately promote, support, and evaluate them. To explain cheating behavior, research seldom focuses on perceived teachers’ characteristics. Thus, we investigate the relationship between students’ cheating behavior and an important teacher characteristic, individual reference norm orientation (IRNO; i.e., the tendency to evaluate students based on their performance development over time). We examined cheating on written exams, on homework, and in oral exams among N = 601 students (64.2% girls; Mage = 16.07 years) in N = 31 language classes. Results from doubly manifest multi-level analyses showed that, on the classroom level, cheating on written exams and on homework occurred less frequently the more the classroom of students perceived their teachers as having an IRNO. We found no further evidence for other cheating factors or student characteristics. This supports the idea that teacher characteristics are associated with some forms of students’ cheating behavior.
Hardships in Italian Prisons During the COVID-19 Emergency: The Experience of Healthcare Personnel
Authors: Testoni Ines , Francioli Giada , Biancalani Gianmarco , Libianchi Sandro , Orkibi Hod
Published: February 2021

Background: The recent COVID-19 pandemic has highlighted the deficiencies that characterize the functioning of the Italian national health system. Prisons have always mirrored the most radical expressions of these weaknesses. During the early stages of the pandemic, prison facilities across Italy underwent a series of changes dictated by the need to ensure the safety of the prisoners and staff. The adoption of these rules contributed to a total or partial redefinition of many central facets of life in prison, such as intake procedures for new arrivals and the ways prisoners were allowed to communicate with their families.

Objectives: The aim of this qualitative study was to analyze the testimony of penitentiary healthcare workers in prisons throughout Italy to determine the impact of COVID-19 on their professional and personal lives.

Participants: Thirty-eight participants were contacted and 20 decided to participate in the interview. The sample was made up of 10 women and 10 men. All the participants were members of the healthcare staff of a penitentiary facility (psychologists, psychiatrists, physicians, and nurses). All were recruited through an Italian association whose mission is the development, promotion, and implementation of social solidarity projects including prisoners' social and health care. This study was facilitated through representatives serving in nine different regions of Italy. The participants were divided according to their professional roles in prisons.

Method: In-depth interviews were conducted by telephone or online using telecommunication platforms (e.g., Zoom, WhatsApp, and Skype). The transcribed texts underwent thematic analysis using the Atlas.ti software to identify patterns of meaning across the dataset.

Results: Four main themes emerged from the analysis: Interpersonal difficulties, management and operational difficulties, the personal distress and bereavement of healthcare workers, and the distress of inmates. The importance of relationship management skills when interacting with prisoners emerged as a key topic in many interviews, and the participants highlighted the need for adequate training. The increase in prisoners' anxiety made communication more difficult.

Conclusions: The findings suggest that healthcare workers in jails need emergency-oriented training. Participants described their feeling of loneliness and quasi-abandonment when carrying out their duties during the pandemic. In particular, they underscored the need for psychological guidance to better manage altered reactions with prisoners and colleagues as a result of heightened death anxiety and isolation.
Teacher Written Feedback on English as a Foreign Language Learners’ Writing: Examining Native and Nonnative English-Speaking Teachers’ Practices in Feedback Provision
Authors: Cheng Xiaolong , Zhang Lawrence Jun
Published: February 2021

While previous studies have examined front-line teachers’ written feedback practices in second language (L2) writing classrooms, such studies tend to not take teachers’ language and sociocultural backgrounds into consideration, which may mediate their performance in written feedback provision. Therefore, much remains to be known about how L2 writing teachers with different first languages (L1) enact written feedback. To fill this gap, we designed an exploratory study to examine native English-speaking (NES) and non-native English-speaking (NNES) (i.e., Chinese L1) teachers’ written feedback practices in the Chinese tertiary context. Our study collected 80 English as a foreign language (EFL) students’ writing samples with teacher written feedback and analyzed them from three aspects: Feedback scope, feedback focus, and feedback strategy. The findings of our study revealed that the two groups of teachers shared similar practices regarding feedback scope and feedback strategies. Both NES and NNES EFL teachers used a comprehensive approach to feedback provision, although NNES teachers provided significantly more feedback points than their NES peers and they delivered their feedback directly and indirectly. However, their practices differed greatly with regard to feedback focus. Specifically, when responding to EFL students’ writing, NES teachers showed more concern with global issues (i.e., content and organization), whereas NNES teachers paid more attention to linguistic errors. With a surge in the recruitment of expatriate NES and local NNES English teachers in China and other EFL countries, our study is expected to make a contribution to a better understanding of the two groups of EFL teachers’ pedagogical practices in written feedback provision and generate important implications for their feedback provision.
Personality and Attitudes Confronting Death Awareness During the COVID-19 Outbreak in Italy and Spain
Authors: Murphy Mike , Moret-Tatay Carmen
Published: February 2021

Italy and Spain are two representative examples on strict lockdown last March 2020, also suffering a high rate of mortality in Europe. The aim of this study is to examine their attitudes confronting death awareness during the Covid-19 outbreak. Moreover, Personality was also considered. Different sociodemographic, in situ questions related to attitudes and the brief Big Five of Personality were employed in a cross-sectional design. The main results suggested that Personality traits were stable across countries. A relationship was found between Fear to contagious diseases and Neuroticism and other attitudes during the Covid-19 outbreak, and two different clusters were identified with regards to attitudes, however these did not differ on Personality. Finally, a Cluster group, Neuroticism, Age and Sense of belonging to the Country did predict Fear to contagious diseases. Of note, no differences were found across countries during grief.
Widespread Decrease of Cerebral Vimentin-Immunoreactive Astrocytes in Depressed Suicides
Authors: O'Leary Liam Anuj , Belliveau Claudia , Davoli Maria Antonietta , Ma Jie Christopher , Tanti Arnaud , Turecki Gustavo , Mechawar Naguib
Published: February 2021

Post-mortem investigations have implicated cerebral astrocytes immunoreactive (-IR) for glial fibrillary acidic protein (GFAP) in the etiopathology of depression and suicide. However, it remains unclear whether astrocytic subpopulations IR for other astrocytic markers are similarly affected. Astrocytes IR to vimentin (VIM) display different regional densities than GFAP-IR astrocytes in the healthy brain, and so may be differently altered in depression and suicide. To investigate this, we compared the densities of GFAP-IR astrocytes and VIM-IR astrocytes in post-mortem brain samples from depressed suicides and matched non-psychiatric controls in three brain regions (dorsomedial prefrontal cortex, dorsal caudate nucleus and mediodorsal thalamus). A quantitative comparison of the fine morphology of VIM-IR astrocytes was also performed in the same regions and subjects. Finally, given the close association between astrocytes and blood vessels, we also assessed densities of CD31-IR blood vessels. Like for GFAP-IR astrocytes, VIM-IR astrocyte densities were found to be globally reduced in depressed suicides relative to controls. By contrast, CD31-IR blood vessel density and VIM-IR astrocyte morphometric features in these regions were similar between groups, except in prefrontal white matter, in which vascularization was increased and astrocytes displayed fewer primary processes. By revealing a widespread reduction of cerebral VIM-IR astrocytes in cases vs. controls, these findings further implicate astrocytic dysfunctions in depression and suicide.
Health Privacy Information Self-Disclosure in Online Health Community
Authors: Yuchao Wang , Ying Zhou , Liao Zangyi
Published: February 2021

The scarcity of medical resources is a fundamental problem worldwide; the development of information technology and the Internet has given birth to online health care, which has alleviated the above problem. The survival and sustainable development of the online health community requires users to continuously disclose their health and privacy. Therefore, it is a great practical significance to find out the factors and mechanisms that promote users' self-disclosure in the online health community. From the perspective of individual and situation interaction, this study constructed influencing factors model of health privacy information self-disclosure. Finally, we collected 264 valid samples from the online health community through online and offline questionnaire surveys and then use the SPSS20.0 and AMOS21.0 to conduct exploratory factor analysis, confirmatory factor analysis, scale reliability and validity analysis, and structural equation model analysis. The main findings are as follows: trust in websites and trust in doctors reduce the privacy concern. The privacy trade-off will not occur when trust is enough to offset the privacy concerns caused by personalized services, reciprocity norms, and other factors. Second, reciprocity norms are inevitably compulsive, which will increase privacy concerns. However, based on voluntariness, reciprocity norms can enhance user trust. Third, service quality caused by personalized services not only enhance the social rewards of users but also eliminate the privacy concern. Fourth, users' health privacy attention and information sensitivity are too high to decrease the influence of user' privacy concerns on personal health privacy information disclosure. The conclusions of this paper will help us to supplement privacy calculus theory and the application scope of the attention-based view. The proposed strategy of this article can be used to stimulate the information contribution behavior of users and improve the medical service capabilities in online health community.
Levels of Physical Activity in the Adult Population of La Guajira, Colombia: A Focus on Ethnicity
Authors: Panciera-di-Zoppola Yaina , Niño-Restrepo Juan , Melo-Freile José , Ortiz-Moncada Rocío
Published: February 2021

The objective of this study is to analyze the physical activity levels among the ethnic groups in La Guajira, Colombia, according to the different ethnic groups and their sociodemographic factors. With regards to ethnic groups, two groups were studied, ethnic (Indigenous and Afro-Colombian) and non-ethnic (White or Mestizo). In order to obtain the relevant data a non-probability sample of 251 people of ≥18 years of age were asked to complete the short version of the International Physical Activity Questionnaire (IPAQ). In this analysis, physical activity (METs.min−1) levels were considered as the dependent variable, while the independent variables were linked to the sociodemographic factors: sex, age, social class, civil status, educational level, and municipality of residence. The individuals were then categorized based on their physical activity levels and their compliance with the World Health Organization's (WHO) physical activity recommendations. Next, using the sociodemographic variables, regression models were made to determine the likelihood of the participants meeting these physical activity targets; these models found that 78.1% of the studied participants met the targets. According to the results of the bivariate analysis, participants of ≥47 years of age, and those with only a primary education presented a lower probability of complying with the physical activity recommendations, while those who lived in large municipalities (Riohacha) displayed a larger probability of compliance [OR = 2.16; 95% CI: (1.18–3.96)]. Furthermore, the multivariate analysis showed that Indigenous and Afro-Colombian people in a low social class are more likely to comply with the physical activity recommendations [ORad = 4.88; 95% CI: (1.31–18.1)], while residing in a smaller municipality (Manaure) is associated with a lower probability of compliance [ORad = 0.39; 95% CI: (0.16–0.91)]. In addition, the educated participants were shown to be active and to comply with the physical activity recommendations is to a high degree, even when compared with national and global results. In the ethnic group however, low social class stood out as the sociodemographic factor most associated with high physical activity, while Afro-Colombians were shown to be more active than the Indigenous people. The results of this study offer evidence that can either form a base for future research.
Statistical Analysis of Magnetic Field Fluctuations in Coronal Mass Ejection-Driven Sheath Regions
Authors: Kilpua E. K. J. , Good S. W. , Ala-Lahti M. , Osmane A. , Fontaine D. , Hadid L. , Janvier M. , Yordanova E.
Published: February 2021

We report a statistical analysis of magnetic field fluctuations in 79 coronal mass ejection- (CME-) driven sheath regions that were observed in the near-Earth solar wind. Wind high-resolution magnetic field data were used to investigate 2 h regions adjacent to the shock and ejecta leading edge (Near-Shock and Near-LE regions, respectively), and the results were compared with a 2 h region upstream of the shock. The inertial-range spectral indices in the sheaths are found to be mostly steeper than the Kolmogorov −5/3 index and steeper than in the solar wind ahead. We did not find indications of an f−1 spectrum, implying that magnetic fluctuation properties in CME sheaths differ significantly from planetary magnetosheaths and that CME-driven shocks do not reset the solar wind turbulence, as appears to happen downstream of planetary bow shocks. However, our study suggests that new compressible fluctuations are generated in the sheath for a wide variety of shock/upstream conditions. Fluctuation properties particularly differed between the Near-Shock region and the solar wind ahead. A strong positive correlation in the mean magnetic compressibility was found between the upstream and downstream regions, but the compressibility values in the sheaths were similar to those in the slow solar wind (<0.2), regardless of the value in the preceding wind. However, we did not find clear correlations between the inertial-range spectral indices in the sheaths and shock/preceding solar wind properties, nor with the mean normalized fluctuation amplitudes. Correlations were also considerably lower in the Near-LE region than in the Near-Shock region. Intermittency was also considerably higher in the sheath than in the upstream wind according to several proxies, particularly so in the Near-Shock region. Fluctuations in the sheath exhibit larger rotations than upstream, implying the presence of strong current sheets in the sheath that can add to intermittency.
Veterinary Interventions to Improve Neonatal Survival on British Beef and Sheep Farms: A Qualitative Study
Authors: Adam Katherine E. , Bruce Ann , Corbishley Alexander
Published: February 2021

Neonatal lamb and calf deaths are a major issue in UK agriculture. Consistent mortality rates over several decades, despite scientific advances, indicate that socioeconomic factors must also be understood and addressed for effective veterinary service delivery to improve lamb and calf survival. This qualitative study utilised semi-structured interviews with vets and farmers to explore the on-farm mechanisms and social context, with a particular focus on the role of the vet, to manage and reduce neonatal losses in beef calves and lambs on British farms. Data were analysed using a realist evaluation framework to assess how the mechanisms and context for veterinary service delivery influence survival as the outcome of interest. A lack of a clear outcome definition of neonatal mortality, and the financial, social and emotional impact of losses on both vets and farmers, are barriers to recording of losses and standardisation of acceptable mortality levels at a population level. Despite this, there appears to be an individual threshold on each farm at which losses become perceived as problematic, and veterinary involvement shifts from preventive to reactive mechanisms for service delivery. The veterinarian-farmer relationship is central to efforts to maximise survival, but the social and economic capital available to farmers influences the quality of this relationship. Health inequalities are well-recognised as an issue in human healthcare and the findings indicate that similar inequalities exist in livestock health systems.
Detection of Epithelial Cell Adhesion Molecule in Feline Normal and Tumor Cell Lines and Tissues With Selected Commercial Anti-human EpCAM Antibodies
Authors: Heyward Christa Y. , Dong Lynn , Shakhzadyan Hayk , Wan Christopher , Stokol Tracy
Published: February 2021

Epithelial cell adhesion molecule (EpCAM) is a transmembrane protein expressed at intercellular junctions in epithelial cells. As an epithelial biomarker, it used for immunologic-based capture of epithelial-derived circulating tumor cells (CTCs) in human patients with different carcinomas. EpCAM expression has not been described in normal or neoplastic epithelial tissues in cats. Our goal was to find a commercial antibody that recognizes surface EpCAM expression for CTC detection. We tested two anti-human EpCAM antibodies, designated for use with flow cytometry, for detection of surface EpCAM expression on feline cell lines derived from normal mammary and renal epithelia and mammary and oropharyngeal squamous cell carcinomas in cats. Only one of the antibodies, a goat polyclonal antibody, labeled normal and neoplastic feline mammary epithelial cells and oropharyngeal squamous cell carcinoma cells; no labeling was observed for normal feline kidney epithelial cells. At low dilution, this antibody immunohistochemically stained the intercellular junctions of normal pancreatic, intestinal and mammary epithelium, as well as neoplastic mammary epithelium in feline tissues; however, oral mucosa, skin, and an oropharyngeal squamous cell carcinoma showed no positive immunostaining. The antibody only weakly bound feline squamous cell carcinoma cell lines under static adhesion. Our results indicate that EpCAM is expressed in specific epithelia in cats but is variably expressed in feline mammary tumors and oropharyngeal squamous cell carcinoma. A higher avidity cross-reactive or feline-specific antibody will be required to further investigate EpCAM expression in normal and neoplastic feline tissue or for detecting CTCs in the blood of tumor-bearing cats.
The Year That Has Passed Us By: Animals in Our Life of COVID-19
Authors: Fine Aubrey H.
Published: February 2021

The year 2020 has been a dreadful one: a living nightmare that has changed and impacted the lives of many [...]
ΔMST and the Regulation of Cardiac CSE and OTR Expression in Trauma and Hemorrhage
Authors: Trautwein Britta , Merz Tamara , Denoix Nicole , Szabo Csaba , Calzia Enrico , Radermacher Peter , McCook Oscar
Published: February 2021

Genetic deletion of 3-mercaptopyruvate sulfurtransferase (MST) is known to result in hypertension and cardiac hypertrophy in older mice, and is associated with increased anxiety-like behaviors. Endogenous hydrogen sulfide (H2S) produced by MST in the mitochondria is also known to be involved in physiological and cellular bioenergetics, and its dysfunction associated with depressive behavior and increased cardiovascular morbidity. Interestingly, early life stress has been shown to lead to a significant loss of cystathionine-γ-lyase (CSE) and oxytocin receptor (OTR) expression in the heart. Thus, we were interested in testing the hypothesis of whether genetic MST mutation (ΔMST) would affect cardiac CSE and OTR expression and affect the mitochondrial respiration in a clinically relevant, resuscitated, mouse model of trauma and hemorrhagic shock. In ΔMST mice, we found a reduction of CSE and OTR in both the naive as well as injured state, in contrast to the wild type (wt) controls. Interestingly, the ΔMST showed a different complex IV response to injury than the wt controls, although our claims are based on the non-demonstrated assumption that naive wt and naive ΔMST mice have comparable complex IV activity. Finally, hemorrhagic shock led to a reduction of CSE and OTR, confirming previous results in the injured mouse heart. To date, the exact mechanisms of the cardiac interaction between H2S and OT are not clear, but they point the way to potential cardioprotective therapies.
Pan-Cancer Analysis Shows TP53 Mutations Modulate the Association of NOX4 with Genetic Programs of Cancer Progression and Clinical Outcome
Authors: Ma Wei Feng , Boudreau Howard E. , Leto Thomas L.
Published: February 2021

Previously, we have shown TGF-β-induced NOX4 expression is involved in the epithelial-to-mesenchymal transition (EMT), a process critical for cancer metastasis, and that wild-type (WT) and mutant (Mut) p53 have divergent effects on TGF-β induction of NOX4: WT-p53 suppresses whereas Mut-p53 augments NOX4 mRNA and protein production in several tumor cell models. We sought to validate and extend our model by analyzing whole-exome data of primary tumor samples in The Cancer Genome Atlas (TCGA). We constructed a Pan-Cancer dataset from 23 tumor types and explored NOX4 expression patterns in relation to EMT and patient survival. NOX4 mRNA levels increase as a function of cancer progression in several cancers and correlate with Mut-p53 mRNA and genes involved in programs of EMT, cellular adhesion, migration, and angiogenesis. Tumor macrophages appear to be a source of NOX2, whose association with genetic programs of cancer progression emulate that of NOX4. Notably, increased NOX4 expression is linked to poorer survival in patients with Mut-TP53, but better survival in patients with WT-TP53. NOX4 is negatively associated with markers of apoptosis and positively with markers of proliferation in patients with Mut-TP53, consistent with their poorer survival. These findings suggest that TP53 mutations could “switch” NOX4 from being protective and an indicator of good prognosis to deleterious by promoting programs favoring cancer progression.
Genistein Prevents Nitric Oxide Deficiency-Induced Cardiac Dysfunction and Remodeling in Rats
Authors: Poasakate Anuson , Maneesai Putcharawipa , Rattanakanokchai Siwayu , Bunbupha Sarawoot , Tong-Un Terdthai , Pakdeechote Poungrat
Published: February 2021

Genistein is an isoflavone found in soybeans. This study evaluates the protective effects of genistein on Nω-nitro-L-arginine methyl ester hydrochloride (L-NAME)-induced hypertension, cardiac remodeling, and dysfunction in rats. Male Wistar rats were treated with L-NAME 40 mg/kg/day together for 5 weeks, with or without genistein at a dose of 40 or 80 mg/kg/day or lisinopril 5 mg/kg/day (n = 8 per group). Genistein prevented L-NAME-induced hypertension in rats. Increases in the left ventricular weight, metalloproteinase-2, metalloproteinase-9, and collagen type I intensity were observed in L-NAME rats, and these changes were attenuated in the genistein-treated group. Genistein reduced circulating angiotensin-converting enzyme activity and angiotensin II concentrations in L-NAME rats. L-NAME increased plasma and cardiac malondialdehyde and vascular superoxide generations, as well as reductions of serum and cardiac catalase activities in rats. Plasma nitrate/nitrite were protected in the genistein-treated group. Genistein prevented the L-NAME-induced overexpression of angiotensin II receptor type I (AT1R), nicotinamide adenine dinucleotide phosphate (NADPH) oxidase subunit 2 (gp91phox), and transforming growth factor beta I (TGF-β1) in hypertensive rats. In conclusion, genistein exhibited a cardioprotective effect in hypertensive rats in this study. The molecular mechanisms might be mediated by suppression of oxidative stress through the Ang II/AT1R/NADPH oxidase/TGF-β1 signaling pathway.
Oxidized High-Density Lipoprotein Shows a Stepwise Increase as Fibrosis Progresses in Patients with Nonalcoholic Fatty Liver Disease
Authors: Miura Kouichi , Arai Naoshi , Goka Rie , Morimoto Naoki , Watanabe Shunji , Isoda Norio , Yamamoto Hironori , Kotani Kazuhiko
Published: February 2021

Patients with nonalcoholic fatty liver disease (NAFLD) show dyslipidemia and a high risk for coronary heart disease (CHD). However, conventional atherosclerotic lipids are found at low levels in NAFLD patients with advanced fibrosis, in whom the risk for CHD is extremely high. The aim of the present study was to evaluate the levels of oxidized high-density lipoprotein (oxHDL), an emerging atherosclerotic biomarker, in patients with NAFLD. A total of 32 non-NAFLD subjects and 106 patients with NAFLD were enrolled. The fibrosis grades were stratified using non-invasive methods, including the Fibrosis-4 index and NAFLD fibrosis score. Total cholesterol and low-density lipoprotein (LDL)-cholesterol levels were significantly low in patients with advanced liver fibrosis. In contrast, oxHDL levels were high in NAFLD patients and showed a stepwise increase as fibrosis progressed. These oxHDL levels were independent of the HDL cholesterol levels, and statin use did not influence the oxHDL levels. Obese patients showed no increase in oxHDL levels, whereas patients with a low handgrip strength showed high oxHDL levels in NAFLD with advanced fibrosis. In conclusion, oxHDL is a potential biomarker for assessing the status of patients with NAFLD, including CHD and metabolic/nutritional disturbance, and particular cases with advanced liver fibrosis.
Relevance of Autophagy and Mitophagy Dynamics and Markers in Neurodegenerative Diseases
Authors: Giorgi Carlotta , Bouhamida Esmaa , Danese Alberto , Previati Maurizio , Pinton Paolo , Patergnani Simone
Published: February 2021

During the past few decades, considerable efforts have been made to discover and validate new molecular mechanisms and biomarkers of neurodegenerative diseases. Recent discoveries have demonstrated how autophagy and its specialized form mitophagy are extensively associated with the development, maintenance, and progression of several neurodegenerative diseases. These mechanisms play a pivotal role in the homeostasis of neural cells and are responsible for the clearance of intracellular aggregates and misfolded proteins and the turnover of organelles, in particular, mitochondria. In this review, we summarize recent advances describing the importance of autophagy and mitophagy in neurodegenerative diseases, with particular attention given to multiple sclerosis, Parkinson’s disease, and Alzheimer’s disease. We also review how elements involved in autophagy and mitophagy may represent potential biomarkers for these common neurodegenerative diseases. Finally, we examine the possibility that the modulation of autophagic and mitophagic mechanisms may be an innovative strategy for overcoming neurodegenerative conditions. A deeper knowledge of autophagic and mitophagic mechanisms could facilitate diagnosis and prognostication as well as accelerate the development of therapeutic strategies for neurodegenerative diseases.
Uretero-Pelvic Junction Stenosis: Considerations on the Appropriate Timing of Correction Based on an Infant Population Treated with a Minimally-Invasive Technique
Authors: Lima Mario , Di Salvo Niel , Portoraro Andrea , Maffi Michela , Parente Giovanni , Catania Vincenzo Davide , Gargano Tommaso
Published: February 2021

There is no univocal consensus about timing of intervention and best surgical approach for infants with asymptomatic uretero-pelvic junction obstruction (UPJO). We conducted a retrospective analysis of patients undergoing one-trocar-assisted pyeloplasty (OTAP) in a 13 year range period by creating two homogenous groups (indications for surgery were the same for all patients): patients operated on in the first 90 days of life (34 patients; Group 1) and patients operated on between 3 and 12 months of life (34 patients; Group 2). We observed no statistically significant differences between groups in regard to mean operative time, conversion rate to open surgery, mean hospital stay, early complications (urinary leakage) rate and mean antero-posterior diameter (APD) reduction rate. Moreover, no statistical improvement was seen between groups in regard to separate renal function (SRF) at 1-year-follow-up renogram. Thanks to the HSS calculated before and 1 year after surgery, we registered an important improvement in Group I patients (p = 0.023). In our study, there was no significant evidence, in terms of intraoperative data and early postoperative outcomes, between patients who underwent an early pyeloplasty and those who underwent a delayed correction. Nevertheless, we registered a significant improvement in those patients with an impaired SRF that underwent an early surgical correction, especially in terms of urinary flow. Even though this study cannot definitely establish the superiority of early timing of correction, it is evident that further research is needed to clarify this aspect.
Euler’s Numerical Method for Ions Rejection Reassessment of a Defect-Free Synthesized Nanofiltration Membrane with Ultrathin Titania Film as the Selective Layer
Authors: Worou Chabi Noël , Kang Jing , Shen Jimin , Degan Arcadius , Yan Pengwei , Wang Weiqiang , Gong Yingxu , Chen Zhonglin
Published: February 2021

Titanium (Ti) nanoparticles (NPs) were successfully seeded on the platform of a polyacrylonitrile (PAN) ultrafiltration (UF) membrane previously coated with bio-glue (a co-deposition of dopamine hydrochloric bicarbonate buffer having undergone pyrocatechol deprotonation). The tools in vogue, especially field emission scanning electron microscopy (FESEM), energy dispersive spectroscopy (EDS), and atomic force microscopy (AFM), have made it possible to fully characterize the structure of the new organic-inorganic nanofiltration (NF) membrane, namely NF_PAN_Ti. A soft computing model has been applied to make commonplace the complex and implicit extended Nernst–Planck equations that govern the transport of ions through NF membranes. Euler’s numerical method was applied with a small step-size and the results obtained were very interesting. The filtration velocity approach of GUEROUT-ELFORD-FERRY helped to estimate the average pore size of NF_PAN_Ti to rp = 0.538 nm. A six-day test carried out on NF_PAN_Ti demonstrated its long-term stability and showed a steady-rejection rate of 89.3% of MgCl2 salt and permeate flux of 56 L·m−2·h−1. The Euler’ numerical method corroborated perfectly the experimental findings since the relative error was found to be very low at 0.33% for Cl− and 0.09% for Mg2+ (RE << 0.1). These practical prediction tools may henceforth help in the choice and calibration of next-generation NF membranes’ synthesis.
Causal Interaction between FDI, Corruption and Environmental Quality in the MENA Region
Authors: Bardi Wajdi , Hfaiedh Mohamed Ali
Published: February 2021

The present work analyzes the impact of foreign direct investment (FDI) and corruption on the quality of the environment in the MENA region. Indeed, the magnitude of corruption and the quality of institutions are often cited as the main factors affecting the FDI inflow. Here, the Autoregressive Distributed Lag (ARDL) approach was used to examine data on a group of MENA countries from 1990 to 2016. Our findings verify the Environmental Kuznets Curve. Furthermore, the empirical estimates approve the “pollution haven” hypothesis, which postulates that the polluting industrial activities of developed countries shift to developing countries which have less stringent environmental regulations. Based on the study findings, we recommend greater awareness of the harmful effects of corruption among political and economic actors.
An Efficient Codebook Search Algorithm for Line Spectrum Frequency (LSF) Vector Quantization in Speech Codec
Authors: Xue Yuqun , Wang Yongsen , Jiang Jianhua , Yu Zenghui , Zhan Yi , Fan Xiaohua , Qiao Shushan
Published: February 2021

A high-performance vector quantization (VQ) codebook search algorithm is proposed in this paper. VQ is an important data compression technique that has been widely applied to speech, image, and video compression. However, the process of the codebook search demands a high computational load. To solve this issue, a novel algorithm that consists of training and encoding procedures is proposed. In the training procedure, a training speech dataset was used to build the squared-error distortion look-up table for each subspace. In the encoding procedure, firstly, an input vector was quickly assigned to a search subspace. Secondly, the candidate code word group was obtained by employing the triangular inequality elimination (TIE) equation. Finally, a partial distortion elimination technique was employed to reduce the number of multiplications. The proposed method reduced the number of searches and computation load significantly, especially when the input vectors were uncorrelated. The experimental results show that the proposed algorithm provides a computational saving (CS) of up to 85% in the full search algorithm, up to 76% in the TIE algorithm, and up to 63% in the iterative TIE algorithm. Further, the proposed method provides CS and load reduction of up to 29–33% and 67–69%, respectively, over the BSS-ITIE algorithm.
Evaluation and Analysis of Selective Deployment of Power Optimizers for Residential PV Systems
Authors: Sinapis Kostas , Tsatsakis Konstantinos , Dörenkämper Maarten , van Sark Wilfried G. J. H. M.
Published: February 2021

Partial shading is widely considered to be a limiting factor in the performance of photovoltaic (PV) systems applied in urban environments. Modern system architectures, combined with per module deployment of power electronics, have been used to improve performance, especially at heterogeneous irradiance conditions, but they come with a high investment cost. In this paper, another approach is used to evaluate the selective deployment of power optimizers (SDPO), which can operate with a variety of string inverters and can be retrofitted in PV systems suffering from high shading losses. A combination of modelling and outdoor field testing showed the benefits and drawbacks of SDPOs in a variety of shading scenarios. Results suggest that there is an energy yield increase of 1–2% on an annual basis compared to that of a reference system. The exact level of increase depends on the shading patterns and combination scenarios used in this paper.
A Review of Vortex Methods and Their Applications: From Creation to Recent Advances
Authors: Mimeau Chloé , Mortazavi Iraj
Published: February 2021

This review paper presents an overview of Vortex Methods for flow simulation and their different sub-approaches, from their creation to the present. Particle methods distinguish themselves by their intuitive and natural description of the fluid flow as well as their low numerical dissipation and their stability. Vortex methods belong to Lagrangian approaches and allow us to solve the incompressible Navier-Stokes equations in their velocity-vorticity formulation. In the last three decades, the wide range of research works performed on these methods allowed us to highlight their robustness and accuracy while providing efficient computational algorithms and a solid mathematical framework. On the other hand, many efforts have been devoted to overcoming their main intrinsic difficulties, mostly relying on the treatment of the boundary conditions and the distortion of particle distribution. The present review aims to describe the Vortex methods by following their chronological evolution and provides for each step of their development the mathematical framework, the strengths and limits as well as references to applications and numerical simulations. The paper ends with a presentation of some challenging and very recent works based on Vortex methods and successfully applied to problems such as hydrodynamics, turbulent wake dynamics, sediment or porous flows.
Effect of High Voltage Cold Plasma on Oxidation, Physiochemical, and Gelling Properties of Myofibrillar Protein Isolate from Asian Sea Bass (Lates calcarifer)
Authors: Olatunde Oladipupo Odunayo , Singh Avtar , Shiekh Khursheed Ahmad , Nuthong Pornpot , Benjakul Soottawat
Published: February 2021

The effects of in-bag dielectric barrier discharge high voltage cold plasma (IB-DBD-HVCP) on myofibrillar protein isolate (MPI) from Asian sea bass (ASB) and its impact on the physiochemical and gelling properties of MPI gels were elucidated. A mixture of argon (90%) and oxygen (10%) was used for generating IB-DBD-HVCP. MPI was subjected to IB-DBD-HVCP for varying times (5–15 min). Total carbonyl content was increased, while total sulfhydryl content was decreased in MPI, especially with augmenting treatment time (TT) (p < 0.05). Surface hydrophobicity initially increased when IB-DBD-HVCP TT of 5 min (DBD-HVCP5) was implemented, followed by subsequent decrease with increasing TT. Based on gel electrophoresis, lower actin and myosin heavy chain (MHC) band intensities were found for MPI subjected to IB-DBD-HVCP, particularly when a TT longer than 10 min was used, compared to those of the control. Gel made from DBD-HVCP5 had higher breaking force, deformation, and highest G′ value compared to others. A more ordered and fibrous network was found in DBD-HVCP5 treated gel. Therefore, IB-DBD-HVCP treatment, particularly for 5 min, enhanced cross-linking of proteins in ASB myofibrillar proteins, which resulted in the improved gel elasticity and strength.