bluuebunny/crossref_metadata_2025 huggingface dataset:
Associational susceptibility of crop plants caused by the invasive weed Canadian goldenrod, Solidago canadensis, via local aphid species
Authors: Szabó Attila-Károly , Bálint János , Molnár Attila , Aszalos Szilvia Erzsébet , Fora Ciprian George , Loxdale Hugh David , Balog Adalbert
Published: December 2022

In the present study, field surveys were conducted to detect potential associational susceptibility of crop plants (potato, alfalfa and maize) caused by Canadian goldenrod,Solidago canadensis(L.), one of the most common invasive weeds in Central and Eastern Europe, via plant associated virus vectoring aphids. Assessments were made in two major agricultural land types: crops grown under high input vs. low-input (LIF) conditions, with and without fertilizers and pesticides. The two most frequent aphid species, found both on Canadian goldenrod and crop plants, were the leaf-curling plum aphid,Brachycaudus helichrysiKaltenbach and the glasshouse-potato aphid,Aulacorthum solani(Kaltenbach). Plant viruses in both weed and crop plants were identified using high-throughput sequencing of small RNAs. Peroxidase (POD) enzyme activity was tested in weed and crop plants to connect aphids feeding processes in weeds and crops. In addition, conceptual modeling was used to detect direct relationships between viruses and other crops. The Canadian goldenrod density was only relevant in the LIF regime. Furthermore, its association withB. helichrysiand associational susceptibility was detected only in LIF. In total, 18 viruses comprising 17 plant and one insect virus were detected in Canadian goldenrod, of which 11 were also detected in potato and alfalfa crops. POD activity was high and correlated with high aphid density in both weed and crop plants, suggesting a direct associational susceptibility between these plants through aphid infestation and viral transmission.
Actin depolymerizing factor-based nanomaterials: A novel strategy to enhance E. mitis-specific immunity
Authors: Yu ZhengQing , Xu LiXin , He Ke , Lu MingMin , Yan RuoFeng , Song XiaoKai , Li XiangRui
Published: December 2022

The epidemic of avian coccidiosis seriously threatens the animals’ welfare and the economic gains of the poultry industry. Widespread in avian coccidiosis, Eimeria mitis (E. mitis) could obviously impair the production performance of the infected chickens. So far, few effective vaccines targeting E. mitis have been reported, and the nanovaccines composed of nanospheres captured our particular attention. At the present study, we construct two kinds of nanospheres carrying the recombinant E. mitis actin depolymerizing factor (rEmADF), then the characterization was then analyzed. After safety evaluation, the protective efficacy of rEmADF along with its nanospheres were investigated in chickens. The promoted secretions of antibodies and cytokines, as well as the enhanced percentages of CD4+ and CD8+ T cells were evaluated by the ELISA and flow cytometry assay. In addition, the absolute quantitative real-time PCR (qPCR) assay implied that vaccinations with rEmADF-entrapped nanospheres could significantly reduce the replications of E. mitis in feces. Compared with the rEmADF-loaded chitosan (EmADF-CS) nanospheres, the PLGA nanospheres carrying rEmADF (EmADF-PLGA nanosphers) were more effective in up-regulating weight efficiency of animals and generated equally ability in controlling E. mitis burdens in feces, suggesting the PLGA and CS nanospheres loaded with rEmADF were the satisfactory nanovaccines for E. mitis defense. Collectively, nanomaterials may be an effective antigen delivery system that could help recombinant E. mitis actin depolymerizing factor to enhance immunoprotections in chicken against the infections of E. mitis.
Testing experimental devices in the extension piece to increase the selectivity of bottom trawl in the Nw Mediterranean
Authors: Sbrana Mario , De Carlo Francesco , Ligas Alessandro , Massaro Andrea , Musumeci Claudia , Rossetti Ilaria , Sartini Marina , Vasapollo Claudio , Viva Claudio , Sartor Paolo , Pretti Carlo
Published: December 2022

In the Mediterranean, experiments and technical measures aimed at improving the selectivity of bottom trawl nets mostly concern modifications to the codend. Grids have been tested in various areas, but have not been adopted for management purposes so far. The present study aims to evaluate whether the adoption of diamond meshes turned by 90° (T90 configuration) and a selection grid with 2 cm bar spacing, both placed in the extension piece of a commercial bottom trawl net, can contribute in reducing the capture of specimens under the Minimum Conservation Reference Size (MCRS) for the main commercial species. The results indicated that the T90 configuration has no effect compared to the commercial net commonly used by fishers. The grid showed a reduction of the catches of European hake individuals between 11 and 19 cm Total Length (TL). However, the use of this device reduced the catch of many species, such as red mullet, deep-water rose shrimp and broad tail short fin squid. This reduction was observed also for specimens above MCRS. In conclusion, the T90 applied to the extension piece does not provide any improvement in selectivity, contrary to what has been observed when the T90 is placed at the codend. In contrast, the grid could be a possible way to increase selectivity. However, the use of grid should be further investigated to identify settings that can avoid the loss of a substantial fraction of commercial catch.
Perceptions towards governance of billfish resource use: The lens of artisanal fishers in Kenya
Authors: Kinyua Damaris Njeri , Kadagi Nelly Isigi , Ater Sarah , Osore Melckzedeck , Wamukota Andrew
Published: December 2022

Multiple fisher groups target billfish species, each with different motivations and experiences, which can influence the effectiveness and sustainability of governance approaches. However, limited studies underscore the perceptions of billfish resource users in defining and implementing governance in the Western Indian Ocean region. We conducted 211 semi-structured qualitative interviews between December 2020 and September 2021, to explore how artisanal fishers perceive the performance and sustainability of governance approaches in Kenya, with a focus on billfish. Our findings show that artisanal fishers have adequate knowledge of fishing laws and regulations, as well as governing institutions and their performance. Further, artisanal fishers had a positive attitude and support for fishing rules, managing institutions, and effectiveness of governance intervention. Specifically, the fishers rated Beach Management Units (BMUs) as highly effective in implementing fisheries rules, indicating the involvement of fishers in co-management of fisheries and tendency for governance success and sustainability. This highlights the need to strengthen and support BMUs as an effective governance tool in the co-management of fisheries. We draw attention to our first-time study of the contribution of artisanal billfish fishers to governance of shared fisheries resources. We show that involvement of resource users promotes a bottom-up approach to the co-management of billfish which compliments the current regional and national efforts that have largely focused on commercial fisheries. Our research adds to the scientific body of knowledge on the importance of perceptions in the formation of natural resource governance interventions at varying scales, especially for transboundary species in data-poor areas.
Strength and microscale properties of bamboo fiber-reinforced concrete modified with natural rubber latex
Authors: Althoey Fadi , Awoyera Paul Oluwaseun , Inyama King , Khan Mohammad Arsalan , Mursaleen Mohammad , Hadidi Haitham M. , Najm Hadee Mohammed
Published: December 2022

Development of concrete using alternative materials has become very important in the quest to achieve sustainable development in the built environment. However, it is critical to continually modify concrete mixtures to correct deficiencies of fresh and long-term properties. In this study, natural rubber latex and bamboo fiber were added as constituent materials in concrete, and the effects of the constituents on concrete were explored. Bamboo fiber (BF) and natural rubber latex (NRL) were added in proportions of 0%, 1%, and 1.5%. The study determined the workability (slump) of fresh concrete mixes, strength, and water absorption properties of the hardened samples after curing them in water for 7, 14, and 28 days. The morphology of the concrete samples was explored using SEM-EDX equipment. The results showed that samples having 1% bamboo fiber content and 1% rubber latex had the highest compressive strength among all the presented samples. Furthermore, samples containing equal but lower percentages of both bamboo and NRL had the highest compressive strength comparable to that of the control. This research showed the feasibility of combining bamboo fiber and rubber latex for an alternative eco-friendly construction approach to enhance the performance of conventional concrete in terms of tensile strength and flexural strength properties.
Predictive models for endoscopic disease activity in patients with ulcerative colitis: Practical machine learning-based modeling and interpretation
Authors: Li Xiaojun , Yan Lamei , Wang Xuehong , Ouyang Chunhui , Wang Chunlian , Chao Jun , Zhang Jie , Lian Guanghui
Published: December 2022

Background

Endoscopic disease activity monitoring is important for the long-term management of patients with ulcerative colitis (UC), there is currently no widely accepted non-invasive method that can effectively predict endoscopic disease activity. We aimed to develop and validate machine learning (ML) models for predicting it, which are desired to reduce the frequency of endoscopic examinations and related costs.

Methods

The patients with a diagnosis of UC in two hospitals from January 2016 to January 2021 were enrolled in this study. Thirty nine clinical and laboratory variables were collected. All patients were divided into four groups based on MES or UCEIS scores. Logistic regression (LR) and four ML algorithms were applied to construct the prediction models. The performance of models was evaluated in terms of accuracy, sensitivity, precision, F1 score, and area under the receiver-operating characteristic curve (AUC). Then Shapley additive explanations (SHAP) was applied to determine the importance of the selected variables and interpret the ML models.

Results

A total of 420 patients were entered into the study. Twenty four variables showed statistical differences among the groups. After synthetic minority oversampling technique (SMOTE) oversampling and RFE variables selection, the random forests (RF) model with 23 variables in MES and the extreme gradient boosting (XGBoost) model with 21 variables in USEIS, had the greatest discriminatory ability (AUC = 0.8192 in MES and 0.8006 in UCEIS in the test set). The results obtained from SHAP showed that albumin, rectal bleeding, and CRP/ALB contributed the most to the overall model. In addition, the above three variables had a more balanced contribution to each classification under the MES than the UCEIS according to the SHAP values.

Conclusion

This proof-of-concept study demonstrated that the ML model could serve as an effective non-invasive approach to predicting endoscopic disease activity for patients with UC. RF and XGBoost, which were first introduced into data-based endoscopic disease activity prediction, are suitable for the present prediction modeling.
Outer membrane vesicles: A bacterial-derived vaccination system
Authors: Lieberman Linda A.
Published: December 2022

Outer membrane vesicles (OMVs) are non-living spherical nanostructures that derive from the cell envelope of Gram-negative bacteria. OMVs are important in bacterial pathogenesis, cell-to-cell communication, horizontal gene transfer, quorum sensing, and in maintaining bacterial fitness. These structures can be modified to express antigens of interest using glycoengineering and genetic or chemical modification. The resulting OMVs can be used to immunize individuals against the expressed homo- or heterologous antigens. Additionally, cargo can be loaded into OMVs and they could be used as a drug delivery system. OMVs are inherently immunogenic due to proteins and glycans found on Gram negative bacterial outer membranes. This review focuses on OMV manipulation to increase vesiculation and decrease antigenicity, their utility as vaccines, and novel engineering approaches to extend their application.
Evolutionary history and global spatiotemporal pattern of alfalfa mosaic virus
Authors: Gao Yanling , Fan Guoquan , Cheng Shengqun , Zhang Wei , Bai Yanju
Published: December 2022

Alfalfa mosaic virus (AMV) is an important plant virus causing considerable economic loss to alfalfa production. Knowledge of the evolutionary and demographic history of the pathogen is limited but essential to the development of effective and sustainable pathogen management schemes. In this study, we performed worldwide phylodynamic analyses of AMV based on 154 nucleotide sequences of the coat protein gene, sampled from 1985 to 2020, to understand the epidemiology of this pathogen. Bayesian phylogenetic reconstruction estimates that the crown group of AMV dates back to 1840 (95% credibility interval, 1687–1955). We revealed that AMV continuously evolves at a rate of 4.14 × 10−4 substitutions/site/year (95% credibility interval, 1.04 × 10−4 − 6.68 × 10−4). Our phylogeographic analyses identified multiple migration links between Europe and other regions, implying that Europe played a key role in spreading the virus worldwide. Further analyses showed that the clustering pattern of AMV isolates is significantly correlated to geographic regions, indicating that geography-driven adaptation may be a factor that affects the evolution of AMV. Our findings may be potentially used in the development of effective control strategies for AMV.
Expression, characterization, and immobilization of a novel SGNH esterase Est882 and its potential for pyrethroid degradation
Authors: Zong Wei , Su Wenfeng , Xie Qingfen , Gu Quliang , Deng Xinyi , Ren Yifei , Li He
Published: December 2022

The widely-used pyrethroid pesticides have attracted public attention because of their potentials to cause environmental pollution and toxic effects on non-target organisms. Esterase is a kind of hydrolytic enzyme that can catalyze the cleavage or formation of ester bonds. it plays a pivotal role in the decomposition of pyrethroids and esters containing industrial pollutants through the hydrolysis of ester bonds. Here, a new esterase gene est882 was successfully screened, which encodes Est882, a SGNH family esterase composed of 294 amino acids. It was heterogeneously expressed, identified and immobilized. Multiple sequence alignment showed that Est882 had a typical GDS(X) conserved motif and a catalytic triad composed of Ser79, Asp269 and His275. Phylogenetic analysis showed that Est882 shall belong to a new esterase family. Biochemical characterization demonstrated that the optimum condition was 40°C and pH 9.0. Est882 immobilization was studied with mesoporous silica SBA-15 as the carrier and found to significantly improve the tolerance and stability of Est882. Its optimum pH increased to 10.0 and stabilized within pH 8.0–11.0. Free Est882 can effectively degrade various pyrethroids within 30 min, with a degradation rate above 80%. The immobilized Est882 yet degraded more than 70% of pyrethroids within 30 min. The present study indicated that Est882 has outstanding potential in bioremediation of a pyrethroid-polluted environment. These characteristics endow Est882 with potential values in various industrial applications and hydrolysis of pyrethroid residues.
Exercise blood-drop metabolic profiling links metabolism with perceived exertion
Authors: Opialla Tobias , Gollasch Benjamin , Kuich Peter H. J. L. , Klug Lars , Rahn Gabriele , Busjahn Andreas , Spuler Simone , Boschmann Michael , Kirwan Jennifer A. , Luft Friedrich C. , Kempa Stefan
Published: December 2022

Background: Assessing detailed metabolism in exercising persons minute-to-minute has not been possible. We developed a “drop-of-blood” platform to fulfill that need. Our study aimed not only to demonstrate the utility of our methodology, but also to give insights into unknown mechanisms and new directions.

Methods: We developed a platform, based on gas chromatography and mass spectrometry, to assess metabolism from a blood-drop. We first observed a single volunteer who ran 13 km in 61 min. We particularly monitored relative perceived exertion (RPE). We observed that 2,3-bisphosphoglycerate peaked at RPE in this subject. We next expanded these findings to women and men volunteers who performed an RPE-based exercise protocol to RPE at Fi O 2 20.9% or Fi O 2 14.5% in random order.

Results: At 6 km, our subject reached his maximum relative perceived exertion (RPE); however, he continued running, felt better, and finished his run. Lactate levels had stably increased by 2 km, ketoacids increased gradually until the run’s end, while the hypoxia marker, 2,3 bisphosphoglycerate, peaked at maximum relative perceived exertion. In our normal volunteers, the changes in lactate, pyruvate, ß hydroxybutyrate and a hydroxybutyrate were not identical, but similar to our model proband runner.

Conclusion: Glucose availability was not the limiting factor, as glucose availability increased towards exercise end in highly exerted subjects. Instead, the tricarboxylic acid→oxphos pathway, lactate clearance, and thus and the oxidative capacity appeared to be the defining elements in confronting maximal exertion. These ideas must be tested further in more definitive studies. Our preliminary work suggests that our single-drop methodology could be of great utility in studying exercise physiology.
Salivary metabolic signatures of carotid atherosclerosis in patients with type 2 diabetes hospitalized for treatment
Authors: Sakanaka Akito , Katakami Naoto , Furuno Masahiro , Nishizawa Hitoshi , Omori Kazuo , Taya Naohiro , Ishikawa Asuka , Mayumi Shota , Inoue Moe , Tanaka Isomura Emiko , Amano Atsuo , Shimomura Iichiro , Fukusaki Eiichiro , Kuboniwa Masae
Published: December 2022

Atherosclerosis is a life-threatening disease associated with morbidity and mortality in patients with type 2 diabetes (T2D). This study aimed to characterize a salivary signature of atherosclerosis based on evaluation of carotid intima-media thickness (IMT) to develop a non-invasive predictive tool for diagnosis and disease follow-up. Metabolites in saliva and plasma samples collected at admission and after treatment from 25 T2D patients hospitalized for 2 weeks to undergo medical treatment for diabetes were comprehensively profiled using metabolomic profiling with gas chromatography-mass spectrometry. Orthogonal partial least squares analysis, used to explore the relationships of IMT with clinical markers and plasma and salivary metabolites, showed that the top predictors for IMT included salivary allantoin and 1,5-anhydroglucitol (1,5-AG) at both the baseline examination at admission and after treatment. Furthermore, though treatment induced alterations in salivary levels of allantoin and 1,5-AG, it did not modify the association between IMT and these metabolites (pinteraction > 0.05), and models with these metabolites combined yielded satisfactory diagnostic accuracy for the high IMT group even after treatment (area under curve = 0.819). Collectively, this salivary metabolite combination may be useful for non-invasive identification of T2D patients with a higher atherosclerotic burden in clinical settings.
Acquisition-dependent modulation of hippocampal neural cell adhesion molecules by associative motor learning
Authors: Navarro-López Juan D. , Contreras Ana , Touyarot Katia , Herrero Ana I. , Venero César , Cambon Karine , Gruart Agnés , Delgado-García José M. , Sandi Carmen , Jiménez-Díaz Lydia
Published: December 2022

It is widely accepted that some types of learning involve structural and functional changes of hippocampal synapses. Cell adhesion molecules neural cell adhesion molecule (NCAM), its polysialylated form polysialic acid to NCAM (PSA-NCAM), and L1 are prominent modulators of those changes. On the other hand, trace eyeblink conditioning, an associative motor learning task, requires the active participation of hippocampal circuits. However, the involvement of NCAM, PSA-NCAM, and L1 in this type of learning is not fully known. Here, we aimed to investigate the possible time sequence modifications of such neural cell adhesion molecules in the hippocampus during the acquisition of a trace eyeblink conditioning. To do so, the hippocampal expression of NCAM, PSA-NCAM, and L1 was assessed at three different time points during conditioning: after one (initial acquisition), three (partial acquisition), and six (complete acquisition) sessions of the conditioning paradigm. The conditioned stimulus (CS) was a weak electrical pulse separated by a 250-ms time interval from the unconditioned stimuli (US, a strong electrical pulse). An acquisition-dependent regulation of these adhesion molecules was found in the hippocampus. During the initial acquisition of the conditioning eyeblink paradigm (12 h after 1 and 3 days of training), synaptic expression of L1 and PSA-NCAM was transiently increased in the contralateral hippocampus to the paired CS-US presentations, whereas, when the associative learning was completed, such increase disappeared, but a marked and bilateral upregulation of NCAM was found. In conclusion, our findings show a specific temporal pattern of hippocampal CAMs expression during the acquisition process, highlighting the relevance of NCAM, PSA-NCAM, and L1 as learning-modulated molecules critically involved in remodeling processes underlying associative motor-memories formation.
Case report: A case of novel treatment for retrograde cricopharyngeal dysfunction
Authors: Xie Mengshu , Wen Hongmei , Dou Zulin
Published: December 2022

Retrograde cricopharyngeal dysfunction (R-CPD) is a recently described disorder characterized by an inability to belch, excessive flatulence, unpleasant gurgling noises, and discomfort in the lower neck, chest, and abdomen. Herein, we describe a case of R-CPD in a 19-year-old man. The patient suffered from flatulence and was unable to belch since birth; auxiliary examination of his digestive system was normal. He was diagnosed with R-CPD based on clinical manifestations and laboratory results. He received an injection of botulinum toxin to the cricopharyngeal muscle under ultrasound, catheter balloon, and electromyographic guidance. His symptoms completely resolved 1 week after the injection.
Aberrant brain functional network strength related to cognitive impairment in age-related hearing loss
Authors: Zhu Shaoyun , Song Jiajie , Xia Wenqing , Xue Yuan
Published: December 2022

Purpose

Age-related hearing loss (ARHL) is a major public issue that affects elderly adults. However, the neural substrates for the cognitive deficits in patients with ARHL need to be elucidated. This study aimed to explore the brain regions that show aberrant brain functional network strength related to cognitive impairment in patients with ARHL.

Methods

A total of 27 patients with ARHL and 23 well-matched healthy controls were recruited for the present study. Each subject underwent pure-tone audiometry (PTA), MRI scanning, and cognition evaluation. We analyzed the functional network strength by using degree centrality (DC) characteristics and tried to recognize key nodes that contribute significantly. Subsequent functional connectivity (FC) was analyzed using significant DC nodes as seeds.

Results

Compared with controls, patients with ARHL showed a deceased DC in the bilateral supramarginal gyrus (SMG). In addition, patients with ARHL showed enhanced DC in the left fusiform gyrus (FG) and right parahippocampal gyrus (PHG). Then, the bilateral SMGs were used as seeds for FC analysis. With the seed set at the left SMG, patients with ARHL showed decreased connectivity with the right superior temporal gyrus (STG). Moreover, the right SMG showed reduced connectivity with the right middle temporal gyrus (MTG) and increased connection with the left middle frontal gyrus (MFG) in patients with ARHL. The reduced DC in the left and right SMGs showed significant negative correlations with poorer TMT-B scores (r = −0.596, p = 0.002; r = −0.503, p = 0.012, respectively).

Conclusion

These findings enriched our understanding of the neural mechanisms underlying cognitive impairment associated with ARHL and may serve as a potential brain network biomarker for investigating and predicting cognitive difficulties.
Acidosis in arterial blood gas testing is associated with clinical outcomes after endovascular thrombectomy
Authors: Shao Rui , Liu Lei , Xu Juan , Lan Pengpeng , Wu Guiping , Shi Hongfeng , Li Ruili , Zhuang Yingle , Han Shanshan , Li Yan , Zhao Ping , Xu Min , Tang Ziren
Published: December 2022

Background

Despite recanalization, some of the patients undergoing endovascular thrombectomy (EVT) still suffer from unfavorable outcomes. Patients with poor prognoses are often accompanied by acidosis in arterial blood gas (ABG) testing. We, therefore, explored the ABG testing results in the early phase of recanalization and analyzed their association with poor prognosis.

Patients and methods

We identified all patients with ischemic stroke and successful endovascular recanalization for anterior circulation vessel occlusion between June 2019 and May 2022. ABG testing was performed in all patients within 0–30 min and 8 h after endovascular therapy. We investigated the relationship between the ABG testing results with symptomatic intracerebral hemorrhage (sICH), hemicraniectomy, and mortality.

Results

A total of 123 patients with stroke after endovascular thrombectomy were analyzed. Of those, eight (6.5%) patients had postinterventional sICH. Acidosis was associated with sICH. Decreased HCO3- levels and HCO3- levels at 8 h after EVT were independently related to a higher risk of sICH. Twelve (9.8%) patients underwent hemicraniectomy for postischemic malignant edema and similar results were found for hemicraniectomy. Increased lactate at 8 h after EVT and decreased HCO3- levels at 8 h after EVT were closely associated with hemicraniectomy. Twenty-two (17.9%) patients died within 3 months. Decreased HCO3- levels were independently related to mortality, as were decreased pH levels at 8 h after EVT and decreased HCO3- levels at 8 h after EVT.

Conclusion

Acidosis is associated with clinical outcomes after endovascular therapy and may help to select patients with poor prognosis in the acute early phase of recanalization.
Classification of motor imagery electroencephalogram signals by using adaptive cross-subject transfer learning
Authors: Feng Jin , Li Yunde , Jiang Chengliang , Liu Yu , Li Mingxin , Hu Qinghui
Published: December 2022

Introduction

Electroencephalogram (EEG)-based motor imagery (MI) classification is an important aspect in brain-computer interfaces (BCIs), which bridges between neural system and computer devices decoding brain signals into recognizable machine commands. However, due to the small number of training samples of MI electroencephalogram (MI-EEG) for a single subject and the great individual differences of MI-EEG among different subjects, the generalization and accuracy of the model on the specific MI task may be poor.

Methods

To solve these problems, an adaptive cross-subject transfer learning algorithm is proposed, which is based on kernel mean matching (KMM) and transfer learning adaptive boosting (TrAdaBoost) method. First, the common spatial pattern (CSP) is used to extract the spatial features. Then, in order to make the feature distribution more similar among different subjects, the KMM algorithm is used to compute a sample weight matrix for aligning the mean between source and target domains and reducing distribution differences among different subjects. Finally, the sample weight matrix from KMM is used as the initialization weight of TrAdaBoost, and then TrAdaBoost is used to adaptively select source domain samples that are closer to the target task distribution to assist in building a classification model.

Results

In order to verify the effectiveness and feasibility of the proposed method, the algorithm is applied to BCI Competition IV datasets and in-house datasets. The results show that the average classification accuracy of the proposed method on the public datasets is 89.1%, and the average classification accuracy on the in-house datasets is 80.4%.

Discussion

Compared with the existing methods, the proposed method effectively improves the classification accuracy of MI-EEG signals. At the same time, this paper also applies the proposed algorithm to the in-house dataset, the results verify the effectiveness of the algorithm again, and the results of this study have certain clinical guiding significance for brain rehabilitation.
Vulnerability to chronic stress and the phenotypic heterogeneity of presbycusis with subjective tinnitus
Authors: Ruan Jian , Hu Xiuhua , Liu Yuehong , Han Zhao , Ruan Qingwei
Published: December 2022

Age-related functional reserve decline and vulnerability of multiple physiological systems and organs, as well as at the cellular and molecular levels, result in different frailty phenotypes, such as physical, cognitive, and psychosocial frailty, and multiple comorbidities, including age-related hearing loss (ARHL) and/or tinnitus due to the decline in auditory reserve. However, the contributions of chronic non-audiogenic cumulative exposure, and chronic audiogenic stress to phenotypic heterogeneity of presbycusis and/or tinnitus remain elusive. Because of the cumulative environmental stressors throughout life, allostasis systems, the hypothalamus-pituitary-adrenal (HPA) and the sympathetic adrenal–medullary (SAM) axes become dysregulated and less able to maintain homeostasis, which leads to allostatic load and maladaptation. Brain–body communication via the neuroendocrine system promotes systemic chronic inflammation, overmobilization of energetic substances (glucose and lipids), and neuroplastic changes via the non-genomic and genomic actions of glucocorticoids, catecholamines, and their receptors. These systemic maladaptive alterations might lead to different frailty phenotypes and physical, cognitive, and psychological comorbidities, which, in turn, cause and exacerbate ARHL and/or tinnitus with phenotypic heterogeneity. Chronic audiogenic stressors, including aging accompanying ontological diseases, cumulative noise exposure, and ototoxic drugs as well as tinnitus, activate the HPA axis and SAM directly and indirectly by the amygdala, promoting allostatic load and maladaptive neuroplasticity in the auditory system and other vulnerable brain regions, such as the hippocampus, amygdala, and medial prefrontal cortex (mPFC). In the auditory system, peripheral deafferentation, central disinhibition, and tonotopic map reorganization may trigger tinnitus. Cross-modal maladaptive neuroplasticity between the auditory and other sensory systems is involved in tinnitus modulation. Persistent dendritic growth and formation, reduction in GABAergic inhibitory synaptic inputs induced by chronic audiogenic stresses in the amygdala, and increased dendritic atrophy in the hippocampus and mPFC, might involve the enhancement of attentional processing and long-term memory storage of chronic subjective tinnitus, accompanied by cognitive impairments and emotional comorbidities. Therefore, presbycusis and tinnitus are multisystem disorders with phenotypic heterogeneity. Stressors play a critical role in the phenotypic heterogeneity of presbycusis. Differential diagnosis based on biomarkers of metabonomics study, and interventions tailored to different ARHL phenotypes and/or tinnitus will contribute to healthy aging and improvement in the quality of life.
Isolation and transfection of myenteric neurons from mice for patch-clamp applications
Authors: Kuehs Samuel , Teege Laura , Hellberg Ann-Katrin , Stanke Christina , Haag Natja , Kurth Ingo , Blum Robert , Nau Carla , Leipold Enrico
Published: December 2022

The enteric nervous system (ENS) is a complex neuronal network organized in ganglionated plexuses that extend along the entire length of the gastrointestinal tract. Largely independent of the central nervous system, the ENS coordinates motility and peristalsis of the digestive tract, regulates secretion and absorption, and is involved in immunological processes. Electrophysiological methods such as the patch-clamp technique are particularly suitable to study the function of neurons as well as the biophysical parameters of the underlying ion channels under both physiological and pathophysiological conditions. However, application of the patch-clamp method to ENS neurons remained difficult because they are embedded in substantial tissue layers that limit access to and targeted manipulation of these cells. Here, we present a robust step-by-step protocol that involves isolation of ENS neurons from adult mice, culturing of the cells, their transfection with plasmid DNA, and subsequent electrophysiological characterization of individual neurons in current-clamp and voltage-clamp recordings. With this protocol, ENS neurons can be prepared, transfected, and electrophysiologically characterized within 72 h. Using isolated ENS neurons, we demonstrate the feasibility of the approach by functional overexpression of recombinant voltage-gated NaV1.9 mutant channels associated with hereditary sensory and autonomic neuropathy type 7 (HSAN-7), a disorder characterized by congenital analgesia and severe constipation that can require parenteral nutrition. Although our focus is on the electrophysiological evaluation of isolated ENS neurons, the presented methodology is also useful to analyze molecules other than sodium channels or to apply alternative downstream assays including calcium imaging, proteomic and nucleic acid approaches, or immunochemistry.
Cancer chemopreventive potential of cooked glutinous purple rice on the early stages of hepatocarcinogenesis in rats
Authors: Guo Huina , Punvittayagul Charatda , Vachiraarunwong Arpamas , Phannasorn Warunyoo , Wongpoomchai Rawiwan
Published: December 2022

Cancer prevention using dietary phytochemicals holds great potential, particularly in the alternative treatment of liver cancer. Our previous study found that the methanol extract of cooked purple rice performed various biological functions including antioxidant, anti-inflammatory, and antimutagenic activities in in vitro assays. This study aimed to evaluate the chemopreventive effects of cooked glutinous purple rice extract (CRE) obtained from routine rice cooking method on diethylnitrosamine (DEN)-induced hepatic preneoplastic lesions in rats, along with its inhibitory mechanisms. CRE containing γ-oryzanols and high amounts of polyphenolic compounds, particularly cyanidin-3-glucoside, was fed to rats over a period 15 weeks. Additionally, injections of triple DEN at a concentration of 100 mg/kg BW were administered to rats once a week during the second, third, and fourth weeks of the experiment. The results revealed that CRE did not induce the formation of glutathione S-transferase placental form (GST-P) positive foci as a precancerous lesion during rat hepatocarcinogenesis, indicating non-carcinogenicity. Furthermore, CRE significantly reduced the number and size of GST-P positive foci in DEN-initiated rats. It also modulated microenvironment homeostasis by reducing the number of PCNA positive hepatocytes and by enhancing the number of apoptotic positive hepatocytes in the livers of DEN-initiated rats. Using RT-PCR analysis, CRE decreased the mRNA expression of some proinflammatory mediators, including interleukin-6, interleukin-1 beta, inducible nitric oxide synthase and cyclooxygenase 2, by attenuating the expression of cyclin E, the proliferation marker, while also inducing the expression of the apoptotic gene, Bcl2 associated X. The inhibitory mechanism at the early stages of hepatocarcinogenesis of CRE may be involved with the attenuation of cell proliferation, the enhancement of apoptosis, and the modulation of the proinflammatory system. Anthocyanins, flavonoids, and γ-oryzanol represent a group of promising chemopreventive agents in cooked glutinous purple rice extract. The outcomes of this study can provide an improved understanding of the potential role of the phytochemicals contained in cooked purple glutinous rice with regard to cancer alleviation.
Changes in the global, regional, and national burdens of NAFLD from 1990 to 2019: A systematic analysis of the global burden of disease study 2019
Authors: Wang Dan , Xu Yanbing , Zhu Zizhao , Li Yanliang , Li Xiaowen , Li Yike , Shen Hui , Wu Wei , Liu Yazhuo , Han Cheng
Published: December 2022

Background

Understanding the burdens and trends of non-alcoholic fatty liver disease (NAFLD) is necessary for developing effective intervention strategies. In this study, Global Burden of Disease (GBD) 2019 study data were extracted and analyzed to elucidate trends of NAFLD.

Methods

The prevalence, incidence, disability-adjusted life year (DALY), and death rates of NAFLD in geographic populations worldwide from 1990 to 2019 were extracted from the GBD 2019 study data. The global temporal trend of NAFLD from 1990 to 2019 was evaluated using estimated annual percentage changes (EAPCs) and age-standardized rates.

Results

Globally, between 1999 and 2019, the age-standardized prevalence rate of NAFLD increased, with EAPCs of 0.77 [95% CI (0.69, 0.85)], whereas the DALY and Death rates decreased, with EAPCs of –0.82 [95% CI (–0.92, –0.71)], and –0.67 [95% CI (–0.76, –0.58)], respectively. Geographically, the age-standardized prevalence rate showed the most serious upward trend in high-income North America with an EAPC of 0.98 [95% CI (0.95, 1.02)], and the age-standardized incidence rate showed an upward trend in Central Asia with an EAPC of 3.17 [95% CI (2.2, 2.49)]. The most significant upward trend of DALY and death rates appeared in Eastern Europe, with EAPCs of 4.06 [95% CI (3.31, 4.82)], and 3.36 [95% CI (2.77, 3.96)], respectively. At the country level, the age-standardized rates showed an upward trend in Armenia, Belarus, and Republic of Korea. Regarding age groups, the percentage change of prevalence was the highest in the 40 to 44 group [0.29 (0.26, 0.34)] from 1990 to 2019; the percentage change of incidence was the highest in the 85 to 89 group [0.46 (0.12, 0.71)] from 1990 to 2019; the percentage change of DALY was the highest in the 80 to 84 group [0.25 (0.11, 0.39)] from 1990 to 2019; and the percentage change of death rate was the highest in the 15 to 19 group [0.36 (0.17, 0.60)] from 1990 to 2019. The percentage change of prevalence of liver cancer due to NASH was the highest in the group of 85 to 89, whereas those of incidence, DALY, and death were the highest in the group above 95 from 1990 to 2019. Regarding the sociodemographic index (SDI), the highest age-standardized prevalence, incidence, and Death rates of NAFLD occurred in middle-SDI countries, and the highest DALY rates of NAFLD occurred in low-SDI countries.

Conclusion

Global NAFLD burdens have increased since 1990. Our findings provide a reference for policymakers to reduce the burden of NAFLD, especially in middle and low-SDI countries.
Dietary adherence to the Mediterranean diet pattern in a randomized clinical trial of patients with quiescent ulcerative colitis
Authors: Haskey Natasha , Shim Rachel C. K. , Davidson-Hunt Alexander , Ye Jiayu , Singh Sunny , Dieleman Levinus A. , Jacobson Kevan , Ghosh Sanjoy , Gibson Deanna L.
Published: December 2022

Background

The Mediterranean diet pattern (MDP) is believed to improve health and promote balanced inflammation and metabolism. While unknown, compelling evidence suggests that MDP could benefit patients with inflammatory bowel disease (IBD). We aimed to evaluate the level of diet adherence, diet quality, and nutritional adequacy of the MDP in patients with Ulcerative Colitis (UC).

Methods

Adult participants (n = 32) with quiescent UC were randomized to follow a MDP (n = 18) or Canadian Habitual Diet (CHD) (n = 14) for 12 weeks. The MDP participants received tailored nutrition education from a Registered Dietitian. Demographic, clinical data, medical history, and quality of life were assessed with the Short Inflammatory Bowel Disease Questionnaire (SIBDQ), dietary adherence with the Mediterranean Diet Serving Score (MDSS), diet quality via the Healthy Eating Index-2015 (HEI-2015), and dietary intake (ASA-24) were completed at baseline and week 12.

Results

Participants’ diets were analyzed (MDP n = 15, CHD n = 13). The MDP (n = 10, 67%) achieved a high level of adherence (MDSS score between 16 and 24) vs. CHD (n = 3), (p = 0.030). HEI-2015 significantly increased from baseline to week 12 (p = 0.007) in the MDP and was significantly higher at week 12 compared to the CHD (p = 0.0001). The SIBDQ (bowel domain) showed reductions in the passage of large amounts of gas (p = 0.01) and improvements in tenesmus (p = 0.03) in the MDP. Despite enhanced diet quality and adherence in the MDP, females had inadequate intakes of calcium, iron, vitamin D, vitamin E, and choline and males had inadequate intakes of fiber, vitamin D, vitamin E, and choline. No adverse events were reported.

Conclusion

With nutrition education, high adherence to the MDP was achieved without an increase in bowel symptoms. Following the MDP led to a higher diet quality; however, nutritional inadequacies were identified. Tailored dietary education focusing on nutrients of concern when following the MDP is recommended to ensure nutritional adequacy.

Clinical trial registration

[www.ClinicalTrials.gov], identifier [NCT03053713].
A liquid immunogenic fiducial eluter for image-guided radiotherapy
Authors: Moreau Michele , Richards Geraud , Yasmin-Karim Sayeda , Narang Amol , Deville Curtiland , Ngwa Wilfred
Published: December 2022

Introduction

Fiducials are routinely used to provide image-guidance during radiotherapy. Here, a new nanoparticle-based liquid immunogenic fiducial is investigated for its potential to provide image-guidance, while also enhancing treatment outcomes.

Methods

This fiducial, liquid immunogenic fiducial eluter (LIFE) biomaterial, is formulated with natural biodegradable polymers, chitosan and sodium alginate with radio-sensitizing nanoparticles, and immunoadjuvant like anti-CD40 monoclonal antibody. Once administered intra-tumorally, this liquid smart radiotherapy biomaterial congeals within the calcium rich tumor microenvironment. The potential use of LIFE biomaterial for providing image guidance in magnetic resonance imaging (MRI) and computed tomography (CT) was investigated over different time period in a pre-clinical tumored mouse model.

Results

Results showed that the LIFE biomaterial can provide both MRI contrast and CT imaging contrast over 3-weeks, with gradual decrease of the contrast over time, as the LIFE biomaterial biodegrades. Results also showed the LIFE biomaterial significantly slowed tumor growth and prolonged mice survival (p < 0.0001) over time.

Discussion

The results highlight the potential use of the LIFE biomaterial as a multi-functional smart radiotherapy biomaterial that could be developed and optimized for hypo-fractionated radiotherapy applications and combining radiotherapy with immunoadjuvants.
A nomogram for predicting the HER2 status of circulating tumor cells and survival analysis in HER2-negative breast cancer
Authors: Yang Yuqin , Li Liudan , Tian Wenjing , Qiao Zhen , Qin Qi , Su Liqian , Li Peiqiu , Chen Weirong , Zhao Hong
Published: December 2022

Background

In breast cancer patients with HER2-negative tumors (tHER2-), HER2-positive CTCs (cHER2+) were associated with promising efficacy of HER2-targeted therapy, but controversy has persisted over its prognostic effect. We developed a model including clinicopathologic parameters/blood test variables to predict cHER2 status and evaluated the prognostic value of cHER2+ in tHER2- patients.

Methods

cHER2+ was detected, blood test results and clinicopathological characteristics were combined, and a nomogram was constructed to predict cHER2 status in tHER2- patients according to logistic regression analysis. The nomogram was evaluated by C-index values and calibration curve. Kaplan–Meier curves, log-rank tests, and Cox regression analyses were performed to evaluate the prognostic value of cHER2 status.

Results

TNM stage, white blood cells (WBCs), neutrophils (NEUs), uric acid (UA), De Ritis ratio [aspartate transaminase (AST)/alanine transaminase (ALT)], and high-density lipoprotein (HDL) were found to be associated with cHER2 status in tHER2- patients in univariate logistic regression analysis, in which UA and De Ritis ratio remained significant in multivariate logistic regression analysis. A model combining these six variables was constructed, the C-index was 0.745 (95% CI: 0.630–0.860), and the calibration curve presented a perfect predictive consistency. In survival analysis, patients of the subgroups “with cHER2+/UA-low” (p = 0.015) and “with cHER2+/De Ritis ratio – high” (p = 0.006) had a significantly decreased disease-free survival (DFS).

Conclusions

Our nomogram, based on TNM stage, WBC, NEU, UA, De Ritis ratio, and HDL, may excellently predict the cHER2 status of tHER2- patients. Incorporation with UA and De Ritis ratio may enhance the prognostic value of cHER2 status.
Adjuvant treatment with yupingfeng granules for recurrent respiratory tract infections in children: A systematic review and meta-analysis
Authors: Zhang Lu , Wang Xuqiong , Wang Dong , Guo Yinling , Zhou Xinying , Yu Haiyan
Published: December 2022

Background

Yupingfeng granules (YPFG) contribute to various chronic respiratory infections. Several clinical studies have evaluated its efficacy and safety in treating recurrent respiratory tract infections (RRTIs) in children. However, the evidence for its use has not been conclusively proven.

Objective

The aim of this study was to demonstrate the efficacy and safety of YPFG in the adjuvant treatment of RRTIs in children.

Methods

We searched PubMed, Embase, Web of Science, Cochrane Library, Clinical Trials, Chinese Clinical Trial Registry, Sinomad, China National Knowledge Infrastructure (CNKI), Wanfang Database, and Chinese Scientific Journals Database (VIP) for randomized controlled trials (RCTs) of YPFG adjuvant therapy for children with RRTIs as of September 1, 2022. We screened the literature for inclusion and exclusion criteria, assessed the quality of each included literature, and then extracted data from each study for this systematic review and meta-analysis.

Results

A total of 17 RCTs were included. Data analysis showed that the total clinical response rate in the YPFG group was significantly higher than that in the control group [risk ratio (RR) = 1.18, 95%CI (1.12, 1.24), I2 = 39%, P < 0.00001]. Compared with the control group, three serum immunoglobulin levels were significantly increased in the YPFG group: IgA level [standardized mean difference (SMD) = 1.23, 95%CI (0.68, 1.78), I2 = 95%, P < 0.0001]; IgM level [SMD = 0.85, 95%CI (0.35, 1.35), I2 = 93%, P = 0.0009]; IgG level [SMD = 1.06, 95%CI (0.65, 1.47), I2 = 91%, P < 0.00001]. The TNF-α level was significantly lower in the YPFG group [SMD = −1.03, 95%CI (−1.55, −0.51), I2 = 84%, P = 0.0001] compared with the control group.

Conclusions

In summary, adjuvant YPFG therapy improves clinical efficacy and immunity in children with RRTIs. However, the effectiveness and safety of YPFG remain to be further verified.

Systematic review registration

[https://inplasy.com/inplasy-2022-3-0150/], identifier [INPLASY202230150].