bluuebunny/crossref_metadata_2025 huggingface dataset:
ИССЛЕДОВАТЕЛЬСКИЕ ПОДХОДЫ И ВКЛАД В МОЛДАВСКУЮ ЭТНОЛОГИЮ И ГАГАУЗОВЕДЕНИЕ М.Н. ГУБОГЛО
Authors: Квилинкова Елизавета Николаевна
Published: December 2020

Статья посвящена Михаилу Николаевичу Губогло – известному ученому, профессору, который является одним из основателей российской этносоциологии и гагаузской этнологии. В статье анализируется практическая значимость использованных им новых этносоциологических методов и подходов в контексте гагаузской проблематики. Отмечается, что он исследовал не только вопросы этногенеза гагаузов, но и культуру повседневности этого народа. Рассматривается использованный им в работах подход, с помощью которого он в системе этнологического знания исследовал идентичности гагаузов, социальные функции их повседневности в послевоенный период и др. Констатируется, что примененный им биографический и автобиографический метод позволил проследить судьбу различных элементов соционормативной культуры гагаузов.
Раскрываются основные области вклада М.Н. Губогло в молдавскую этнологию и гагаузоведение. По его идее и под его руководством был успешно реализован ряд крупных масштабных проектов: проведены российско-молдавские симпозиумы, опубликованы коллективные монографии в престижной серии «Народы и культуры», изданы сборники и др. Благодаря приложенным им усилиям был дан мощный импульс молдавской этнологии, активизировались междисциплинарные исследования. Подчеркивается, что он внес неоценимый вклад в развитие молдавского гагаузоведения. Делается вывод о том, что в первом десятилетии XXI века состоялся переход гагаузоведения от описательной этнографии к аналитической этнологии.
The article is dedicated to Mikhail Nikolaevich Guboglo – a famous scientist, professor, who is one of the founders of Russian ethno-sociology and Gagauz ethnology. The article analyzes the practical values of his new ethno-sociological methods and approaches in the context of Gagauz studies. It is noted that he studied not only the ethnogenesis of the Gagauz people, but also their everyday culture and lifestyles. The article examines the approach he developed to investigate the identities of the Gagauzes, the social functions of their everyday life in the post-war and other periods in the system of ethnological knowledge. The author argues that the biographical and autobiographical method he used allowed him to trace the fate of various elements of the socio-normative culture of the Gagauz people.
The main areas of M.N. Guboglo’s contribution to Moldavian ethnology and Gagauz studies are revealed. According to his idea and under his leadership, a number of large-scale projects were successfully implemented: Russian-Moldavian symposia were held, collective monographs were published in the prestigious series “Peoples and Cultures”, collections were printed etc. Thanks to his efforts, a powerful impetus was given to Moldovan ethnology, and interdisciplinary research intensified. It is emphasized that he made an invaluable contribution to the development of Moldavian Gagauz studies. It is concluded that in the first decade of the 21st century, Gagauz studies transformed from descriptive ethnography to analytical ethnology.
Nature Based Solutions for Urban Resilience: A Distinction Between No-Tech, Low-Tech and High-Tech Solutions
Authors: Snep Robbert PH , Voeten Joris GWF , Mol Gerben , Van Hattum Tim
Published: December 2020

Urbanization and extreme weather require smarter urban water management. Nature-based solutions (NBS) like vegetated roofs and city trees can contribute effectively to climate resilience and future proof urban water management. However, large scale implementation is limited due to a lack of knowledge among professionals on how to capture, store, and reuse water on-site. In this paper we advocate a classification into no-tech, low-tech, and high-tech green, thereby supporting urban designers to better utilize the ability of these green elements to effectively manage water flows in different urban settings. Here, “no tech” green is considered traditional urban green, handling (rain) water like nature would. “Low-tech” green (e.g., extensive Sedum roofs) are suitable for dense urban settings with limited demand for water management and ecosystem services. More developed “high-tech” green solutions have vegetation performing even beyond natural capacities, offering full water management control options and enable city planners, architects and landscape designers to enhance urban resilience and circularity without claiming valuable urban space. We elaborate our “tech NBS” approach for city trees and vegetated roofs thereby demonstrating the classification's added value for sustainable urban design. We conclude that specifying the demanded “no/low/high” -tech level of green infrastructure in urban design plans will help to yield the most of ecosystem services using appropriate levels of available technology.
Effect of the AM Fungus Sieverdingia tortuosa on Common Vetch Responses to an Anthracnose Pathogen
Authors: Ding Tingting , Zhang Weizhen , Li Yingde , Duan Tingyu
Published: December 2020

Colletotrichum lentisDamm causes anthracnose inVicia sativaL, otherwise known as common vetch. It was first reported in China in 2019. This study evaluates the effects of the arbuscular mycorrhizal (AM) fungusSieverdingia tortuosa(N.C. Schenck & G.S. Sm.) Błaszk., Niezgoda, & B.T. Goto on growth and disease severity in common vetch. Our main finding is that the AM fungus increased root biomass and reduced anthracnose severity of common vetch. Responses correlated with defense, such as chitinase activity, polyphenol oxidase (PPO) activity, the concentrations of jasmonic acid and proline, and the expression of resistance-related genes (e.g., upregulated “signal transduction,” “MAPK signaling pathway,” “chitinase activity,” “response to stress,” and the KEGG pathways “phenylpropanoid biosynthesis,” “MAPK signaling pathways,” and “plant-pathogen interactions”), were also affected These findings provide insight into the mechanism by which this AM fungus regulates the defense response of common vetch toC. lentis.
Dinoflagellate Host Chloroplasts and Mitochondria Remain Functional During Amoebophrya Infection
Authors: Kayal Ehsan , Alves-de-Souza Catharina , Farhat Sarah , Velo-Suarez Lourdes , Monjol Joanne , Szymczak Jeremy , Bigeard Estelle , Marie Dominique , Noel Benjamin , Porcel Betina M. , Corre Erwan , Six Christophe , Guillou Laure
Published: December 2020

Dinoflagellates are major components of phytoplankton that play critical roles in many microbial food webs, many of them being hosts of countless intracellular parasites. The phototrophic dinoflagellate Scrippsiella acuminata (Dinophyceae) can be infected by the microeukaryotic parasitoids Amoebophrya spp. (Syndiniales), some of which primarily target and digest the host nucleus. Early digestion of the nucleus at the beginning of the infection is expected to greatly impact the host metabolism, inducing the knockout of the organellar machineries that highly depend upon nuclear gene expression, such as the mitochondrial OXPHOS pathway and the plastid photosynthetic carbon fixation. However, previous studies have reported that chloroplasts remain functional in swimming host cells infected by Amoebophrya. We report here a multi-approach monitoring study of S. acuminata organelles over a complete infection cycle by nucleus-targeting Amoebophrya sp. strain A120. Our results show sustained and efficient photosystem II activity as a hallmark of functional chloroplast throughout the infection period despite the complete digestion of the host nucleus. We also report the importance played by light on parasite production, i.e., the amount of host biomass converted to parasite infective propagules. Using a differential gene expression analysis, we observed an apparent increase of all 3 mitochondrial and 9 out of the 11 plastidial genes involved in the electron transport chains (ETC) of the respiration pathways during the first stages of the infection. The longer resilience of organellar genes compared to those encoded by the nucleus suggests that both mitochondria and chloroplasts remain functional throughout most of the infection. This extended organelle functionality, along with higher parasite production under light conditions, suggests that host bioenergetic organelles likely benefit the parasite Amoebophrya sp. A120 and improve its fitness during the intracellular infective stage.
Multidisciplinary Telemedicine Care for Tourette Syndrome: Minireview
Authors: Cen Shan-shan , Yu Jun , Wang Qiao , Deeb Wissam , Wang Kai-liang , Shukla Aparna Wagle , Malaty Irene , Ramirez-Zamora Adolfo , Zhang Jian-guo , Hu Wei , Meng Fan-gang
Published: December 2020

Tourette syndrome (TS) is a childhood-onset, chronic neuropsychiatric disorder characterized by multiple motor and vocal tics. TS poses a considerable burden on both patients and health care providers, leading to a major detriment of educational success, occupation, and interpersonal relationships. A multidisciplinary, specialist-driven management approach is required due to the complexity of TS. However, access to such specialty care is often dramatically limited by the patients' locations and the specialists' geographic clustering in large urban centers. Telemedicine uses electronic information and communication technology to provide and support health care when distance separates participants. Therefore, we conducted this mini-review to describe the latest information on telemedicine in the assessment and management of TS and discuss the potential contributions to care for TS patients with a multidisciplinary approach. We believe that telemedicine could be a revolutionary method in improving medical access to patients with TS.
Microbiota Features Associated With a High-Fat/Low-Fiber Diet in Healthy Adults
Authors: Bailén María , Bressa Carlo , Martínez-López Sara , González-Soltero Rocío , Montalvo Lominchar Maria Gregoria , San Juan Celia , Larrosa Mar
Published: December 2020

A high intake of dietary saturated fatty acids (SFAs) is related to an increased risk of obesity, inflammation and cancer-related diseases, and this risk is attenuated only when SFAs are replaced by unsaturated fats and unrefined carbohydrates. The gut microbiota has recently emerged as a new environmental factor in the pathophysiology of these disorders, and is also one of the factors most influenced by diet. We sought to determine whether the gut microbiota of healthy individuals whose intake of SFAs exceeds World Health Organization (WHO) recommendations exhibits features similar to those reported in people with obesity, inflammation, cancer or metabolic disease. Healthy non-obese subjects were divided into two groups based on their SFAs intake. Body composition and gut microbiota composition were analyzed, and associations between bacterial taxa, diet and body fat composition were determined globally and separately by sex. Metagenome functional pathways were predicted by PICRUSt analysis. Subjects whose SFAs intake exceeded WHO recommendations also had a dietary pattern of low fiber intake. This high saturated fat/low fiber diet was associated with a greater sequence abundance of the Anaerotruncus genus, a butyrate producer associated with obesity. Analysis of data of high SFAs intake by sex showed that females presented with a greater abundance of Campylobacter, Blautia, Flavonifractor and Erysipelatoclostridium, whereas males showed higher levels of Anaerotruncus, Eisenbergiella, a genus from the order Clostridiales (FamilyXIIIUCG_001) and two genera from the Lachnospiraceae family. PICRUSt analysis confirmed these data, showing a correlation with a decrease in the abundance of sequences encoding for transporters of some metals such as iron, which is needed to maintain a healthy metabolism. Thus, the microbiota of healthy people on a high SFAs diet contain bacterial taxa (Anaerotruncus, Lachnospiraceae Flavonifractor, Campylobacter, Erysipelotrichacea and Eisenbergiella) that could be related to the development of some diseases, especially obesity and other pro-inflammatory diseases in women. In summary, the present study identifies bacterial taxa that could be considered as early predictors for the onset of different diseases in healthy subjects. Also, sex differences in gut microbiota suggest that women and men differentially benefit from following a specific diet.
Mitochondrial Deoxyguanosine Kinase Regulates NAD+ Biogenesis Independent of Mitochondria Complex I Activity
Authors: Sang Lei , He Ying-Jie , Kang Jiaxin , Ye Hongyi , Bai Weiyu , Luo Xiao-Dong , Sun Jianwei
Published: December 2020

Overexpression of DGUOK promotes mitochondria oxidative phosphorylation and lung adenocarcinoma progression. However, the role and mechanism of DGUOK in regulation of mitochondria function and lung cancer progression still poorly understood. Here we demonstrated that DGUOK regulated NAD+ biogenesis. Depletion of the DGUOK significantly decreased NAD+ level. Furthermore, knockout of the DGUOK considerably reduced expression of the NMNAT2, a key molecule controlling NAD+ synthesis, at both mRNA and protein levels. Ectopic expression of the NMNAT2 abrogated the effect of knockdown of DGUOK on NAD+. Notably, this regulation is independent of DGUOK -mediated mitochondria complex I activity. We also showed that NMNAT2 was highly expressed in lung adenocarcinoma and negatively correlated with the patient overall survival. Our study suggested that DGUOK regulates NAD+ in a NMNAT2 dependent manner and DGUOK-NMNAT2-NAD+ axis could be a potential therapeutic target in lung adenocarcinoma.
Isoquercitrin Ameliorates Cisplatin-Induced Nephrotoxicity Via the Inhibition of Apoptosis, Inﬂammation, and Oxidative Stress
Authors: Wang Hao , Xia Weiwei , Long Guangfeng , Pei Zhiyin , Li Yuanyuan , Wu Mengying , Wang Qian , Zhang Yue , Jia Zhanjun , Chen Hongbing
Published: December 2020

Cisplatin is extensively used and is highly effective in clinical oncology; nevertheless, nephrotoxicity has severely limited its widespread utility. Isoquercitrin (IQC), a natural flavonoid widely found in herbage, is well known and recognized for its antioxidant, anti-inflammatory, and anti-apoptotic properties. However, the potential effects and mechanism of IQC in cisplatin-induced acute kidney diseases remain unknown. In this study, we postulated the potential effects and mechanism of IQC upon cisplatin exposurein vivoandin vitro. For thein vivostudy, C57BL/6J mice were pretreated with IQC or saline (50 mg/kg/day) by gavage for 3 days before cisplatin single injection (25 mg/kg). Renal function, apoptosis, inflammation, oxidative stress and p-ERK were measured to evaluate kidney injury.In vitro, mouse proximal tubular cells (mPTCs) and human proximal tubule epithelial cell line (HK2) were pretreated with or without IQC (80 μM for mPTCs and 120 μM for HK2) for 2 h and then co-administrated with cisplatin for another 24 h. Apoptosis, inflammation, ROS and p-ERK of cells were also measured.In vivo, IQC administration strikingly reduced cisplatin-induced nephrotoxicity as evidenced by the improvement in renal function (serum creatinine and blood urea nitrogen), kidney histology (PAS staining), apoptotic molecules (cleaved caspase-3, caspase-8, Bax and Bcl-2), inﬂammatory cytokines (IL-1β, IL-6, TNF-α, and COX-2), oxidative stress (MDA and total glutathione) and p-ERK. In line within vivofindings, IQC markedly protected against cisplatin-induced cell injury in mPTCs and HK2 cells. Collectively, these findings demonstrated that IQC administration could significantly protect against cisplatin nephrotoxicity possibly through ameliorating apoptosis, inflammation and oxidative stress accompanied by cross talk with p-ERK. Furthermore, IQC may have potential therapeutic uses in the treatment of cisplatin-induced acute kidney injury.
Elevated Neurokinin-1 Receptor Expression in Uterine Products of Conception Is Associated With First Trimester Miscarriages
Authors: Alwazzan Ahmad , Mehboob Riffat , Hassan Amber , Perveen Shahida , Sadaf  , Gilani Syed Amir , Ahmad Fridoon Jawad , Tanvir Imrana , Babar Masroor Elahi , Tariq Muhammad Akram , Ali Gibran , Akram Shehla Javed , Khan Rizwan Ullah , Akram Javed
Published: December 2020

Background

Miscarriage is a common complication of early pregnancy, mostly occurring in the first trimester. However, the etiological factors and prognostic and diagnostic biomarkers are not well known. Neurokinin-1 receptor (NK-1R) is a receptor of tachykinin peptide substance P (SP) and has a role in various pathological conditions, cancers, but its association with miscarriages and significance as a clinicopathological parameter are not studied. Accordingly, the present study aimed to clarify the localization and expression for NK-1R in human retained products of conception (POC). The role of NK-1R is not known in miscarriages.

Materials and Methods

NK-1R expression was assessed in POC and normal placental tissues by immunohistochemistry. Three- to four-micrometer-thin sections of formalin-fixed paraffin-embedded tissues were used for this purpose. Tissues were processed and then immunohistochemically stained with NK-1R antibody. Brain tissue was used as control for antibody. Protein expression was evaluated using the nuclear labeling index (%). Tissues were counterstained with 3,3′-diaminobenzidine (DAB), and microscopy was performed at 10×, 20×, and 40× magnifications.

Results

Ten human POC tissues and 10 normal placental tissues were studied by immunohistochemistry to demonstrate the localization of NK-1R. The expression of NK-1R protein was high in all the cases of both groups. NK-1R expression showed no notable differences among different cases of miscarriages as well as normal deliveries at full term regardless of the mother’s age and gestational age at which the event occurred. Statistically, no difference was found in both groups, which is in agreement with our hypothesis and previous findings.

Conclusion

The expression of NK-1R was similar in all the cases, and it was intense. It shows that dysregulation of NK-1R along with its ligand SP might be involved in miscarriages and also involved in normal delivery. Our results provide fundamental data regarding this anti-NK-1R strategy. Thus, the present study recommends that SP/NK-1R system might, therefore, be considered as an emerging and promising diagnostic and therapeutic strategy against miscarriages. Hence, we report for the first time the expression and localization of NK-1R in POC. We suggest NK-1R antagonist in addition to the immunoglobulins and human chorionic gonadotropin to diagnose and treat spontaneous miscarriages.
On the Use of the Repeated-Sprint Training in Hypoxia in Tennis
Authors: Brechbuhl Cyril , Brocherie Franck , Willis Sarah J. , Blokker Thomas , Montalvan Bernard , Girard Olivier , Millet Gregoire P. , Schmitt Laurent
Published: December 2020

Purpose

To examine physiological and technical responses to repeated-sprint training in normobaric hypoxia at ∼3,000 m (RSH, n = 11) or in normoxia (RSN, n = 11) compared to a control group (CON, n = 8) in well-trained tennis players. Participants were 28.8 ± 5.9 years old without any previous experience of training in hypoxia.

Methods

In addition to maintaining their usual training (CON), both RSH and RSN groups completed five tennis specific repeated-shuttle sprint sessions (4 × 5 × ∼8 s maximal sprints with ∼22 s passive recovery and ∼5 min rest between sets) over 12 days. Before (Pre), the week after (Post-1) and 3 weeks after Post-1 (Post-2), physical/technical performance during Test to Exhaustion Specific to Tennis (TEST), repeated-sprint ability (RSA) (8 × ∼20 m shuttle runs—departing every 20 s) and heart rate variability (HRV) were assessed.

Results

From Pre to Post-1 and Post-2, RSH improved TEST time to exhaustion (+18.2 and +17.3%; both P < 0.001), while the “onset of blood lactate accumulation” at 4 mmol L–1 occurred at later stages (+24.4 and +19.8%, both P < 0.01). At the same time points, ball accuracy at 100% V̇O2max increased in RSH only (+38.2%, P = 0.003 and +40.9%, P = 0.007). Markers of TEST performance did not change for both RSN and CON. Compared to Pre, RSA total time increased significantly at Post-1 and Post-2 (−1.9 and −2.5%, P < 0.05) in RSH only and this was accompanied by larger absolute Δ total hemoglobin (+82.5 and +137%, both P < 0.001). HRV did not change either supine or standing positions.

Conclusion

Five repeated sprint training sessions in hypoxia using tennis specific shuttle runs improve physiological and technical responses to TEST, RSA, and accompanying muscle perfusion responses in well-trained tennis players.
Ultraviolet Radiation From a Plant Perspective: The Plant-Microorganism Context
Authors: Vanhaelewyn Lucas , Van Der Straeten Dominique , De Coninck Barbara , Vandenbussche Filip
Published: December 2020

Ultraviolet (UV) radiation directly affects plants and microorganisms, but also alters the species-specific interactions between them. The distinct bands of UV radiation, UV-A, UV-B, and UV-C have different effects on plants and their associated microorganisms. While UV-A and UV-B mainly affect morphogenesis and phototropism, UV-B and UV-C strongly trigger secondary metabolite production. Short wave (<350 nm) UV radiation negatively affects plant pathogens in direct and indirect ways. Direct effects can be ascribed to DNA damage, protein polymerization, enzyme inactivation and increased cell membrane permeability. UV-C is the most energetic radiation and is thus more effective at lower doses to kill microorganisms, but by consequence also often causes plant damage. Indirect effects can be ascribed to UV-B specific pathways such as the UVR8-dependent upregulated defense responses in plants, UV-B and UV-C upregulated ROS accumulation, and secondary metabolite production such as phenolic compounds. In this review, we summarize the physiological and molecular effects of UV radiation on plants, microorganisms and their interactions. Considerations for the use of UV radiation to control microorganisms, pathogenic as well as non-pathogenic, are listed. Effects can be indirect by increasing specialized metabolites with plant pre-treatment, or by directly affecting microorganisms.
Influence of Teachers’ Grouping Strategies on Children’s Peer Social Experiences in Early Elementary Classrooms
Authors: Kim Saetbyul , Lin Tzu-Jung , Chen Jing , Logan Jessica , Purtell Kelly M. , Justice Laura M.
Published: December 2020

Most children experience some form of grouping in the classroom every day. Understanding how teachers make grouping decisions and their impacts on children’s social development can shed light on effective teacher practices for promoting positive social dynamics in the classroom. This study examined the influence of teachers’ grouping strategies on changes in young children’s social experiences with peers across an academic year. A total of 1,463 children (51% girls, Mage = 6.79, SDage = 1.22) and 79 teachers from kindergarten to third-grade classrooms participated in this study. Teachers rated children’s behavioral problems as the most important consideration when creating seating charts or assigning children to small groups. Promoting existing or new friendships was rated as the least important consideration. Heterogeneous ability grouping, rated as somewhat important by the teachers, was associated with a decrease in children’s friendships and yet also a decrease in girls’ experience with peer conflicts. Our findings begin to fill in the gaps in the literature on the social impacts of ability grouping for young children.
Is Social Contact With the Resident Population a Prerequisite of Well-Being and Place Attachment? The Case of Refugees in Rural Regions of Germany
Authors: Glorius Birgit , Kordel Stefan , Weidinger Tobias , Bürer Miriam , Schneider Hanne , Spenger David
Published: December 2020

Due to dispersal policies applied in many European countries, such as Germany, rural regions are important arrival regions for asylum seekers and refugees. For German policy makers, who have faced a large number of immigrants since 2015, it is crucial that asylum seekers and refugees stay in those rural regions and benefit the development of those areas. This paper addresses the quality and quantity of social contact between refugees and resident populations as a prerequisite for integration and long-term migration-development effects from a social geographical perspective. Drawing from survey data and qualitative interviews, we examine expectations, perceptions and experiences of everyday encounters and social relationships in neighborhoods in small rural towns and villages from the perspective of both local residents and refugees. Our results support arguments from research literature for faster social inclusion in rural areas due to greater nearness, but also obstacles toward the integration of foreigners due to a higher homogeneity of rural neighborhoods and only few experiences of positive everyday contact with foreigners among rural residents. The interviewed refugees display a high level of reflexivity regarding their new neighborhood and how they might be seen by rural residents. Their experiences encompass various forms of social relationships, while social bridges are crucial, ranging from serendipitous encounters and functional interactions to connections based on mutual interest around family issues or cultural aspects. Openness and tolerance from at least some parts of the local population can help immigrants to feel at home, and support staying aspirations, while simultaneously evoking wider social change. A peculiarity of rural areas is the intersectionality with further challenges related to structural changes, encompassing, for instance, socio-demographic and economic restructuring. However, social interactions and opportunities for encounters are only one factor in the development of long-term settlement. More in-depth research is needed to consider the interrelations of both structural contexts and complex and changing needs for personal development in the future, also from an intergenerational perspective.
Change in Proteolytic Profile in Heifers After Oligofructose Overload
Authors: Li Shuaichen , Zheng Xiaoyan , Ding Mengyuan , Tao Ze , Zhang Jiantao , Zhang Na
Published: December 2020

Laminitis in cattle is an important underlying cause of lameness, which leads to a significant reduction in economic and animal welfare. Nevertheless, the disordered pathological processes of laminitis remain unclear. Several proteinases are probably involved in the disorder of basement membrane (BM) metabolism in laminitis, for instance, matrix metalloproteinases (MMPs), neutrophil elastase (NE), and myeloperoxidase (MPO). This study aimed to investigate the change in proteolytic profile in circulating and lamellar tissues using an oligofructose (OF) overload-induced laminitis model in heifers. Twelve clinically healthy and nonlame Chinese Holstein heifers were recruited and randomly divided into two groups: OF-induced and control (CON). The OF-induced heifers group (n = 6) was administered 17 g/kg of body weight (BW) of OF dissolved in 2 L/100 kg of BW of tap water via the oral-rumen tube. The CON group (n = 6) was given an equal volume of tap water. The plasma samples were collected 0, 6, 12, 18, 24, 36, 48, 60, and 72 h after administration, and the lamellar samples were collected 72 h after euthanasia. The plasma samples were analyzed by zymography and reverse zymography. Histological examination, zymography, reverse zymography, and Western blot of lamellar samples were conducted. In the plasma of the OF-induced group, the pro-MMP9 activity increased from 36 h (P < 0.001) to 60 h (P < 0.05). Moreover, the plasma tissue inhibitors of metalloproteinase 1 (TIMP1) activity decreased after 18 h (P < 0.05), while the ratio of pro-MMP9 to TIMP1 and TIMP2 increased after 18 h (P < 0.001) and 48 h (P < 0.05), respectively. The act-MMP2, pro-MMP9, and act-MMP9 activities increased in the lamellar tissue of the OF-induced group compared with the CON group (P < 0.05). In addition, the expression of lamellar NE protein was higher in the OF-induced group (P < 0.01), while no change was found in lamellar MPO protein compared with the CON group. In conclusion, increased pro-MMP9 combined with decreased TIMP1 activity in the circulation might have caused the activation of blood neutrophils, while the activation of proteolytic enzymes in lamellar tissue probably led to the dysfunction of BM in the OF-induced group.
The Effect of Glutathione Peroxidase-1 Knockout on Anticancer Drug Sensitivities and Reactive Oxygen Species in Haploid HAP-1 Cells
Authors: Behnisch-Cornwell Steven , Wolff Lisa , Bednarski Patrick J.
Published: December 2020

The role of glutathione peroxidases (GPx) in cancer and their influence on tumor prognosis and the development of anticancer drug resistance has been extensively and controversially discussed. The aim of this study was to evaluate the influence of GPx1 expression on anticancer drug cytotoxicity. For this purpose, a GPx1 knockout of the near-haploid human cancer cell line HAP-1 was generated and compared to the native cell line with regards to morphology, growth and metabolic rates, and oxidative stress defenses. Furthermore, the IC50 values of two peroxides and 16 widely used anticancer drugs were determined in both cell lines. Here we report that the knockout of GPx1 in HAP-1 cells has no significant effect on cell size, viability, growth and metabolic rates. Significant increases in the cytotoxic potency of hydrogen peroxide and tert-butylhydroperoxide, the anticancer drugs cisplatin and carboplatin as well as the alkylating agents lomustine and temozolomide were found. While a concentration dependent increases in intracellular reactive oxygen species (ROS) levels were observed for both HAP-1 cell lines treated with either cisplatin, lomustine or temozolamide, no significant enhancement in ROS levels was observed in the GPx1 knockout compared to the native cell line except at the highest concentration of temozolamide. On the other hand, a ca. 50% decrease in glutathione levels was noted in the GPx1 knockout relative to the native line, suggesting that factors other than ROS levels alone play a role in the increased cytotoxic activity of these drugs in the GPx1 knockout cells.
Numerical Study on the Evolution Mechanism of the Crater under a Millisecond Laser
Authors: Zhu Dongpo , Zhang Peiyun , Tian Zhixiang , Chen Cheng , Hua Xijun , Xu Sheng , Xie Xuan
Published: December 2020

A two-dimensional numerical model considering recoil pressure and Hertz-Knudsen ablation rate was established on the foundation of the laser remelting model to investigate the influence of laser processing parameters on crater feature and melted zone, and it was verified through experiments. The temperature and flow velocity distribution of the molten pool during the formation of the crater were analyzed. The results showed that the ablation velocity could be considered under a higher laser peak power density or higher pulse width due to the metal evaporation caused by heat accumulation. The depth and diameter of the crater were significantly affected by laser peak power density and laser pulse duration. Simultaneously, the height of the edge bulge decreased with the increase in pulse duration after 1.5 ms, and the growth rate of central depth was more rapid than that of edge bulge height with the increase of laser peak power density. In the texture with the same depth, a larger melted zone could be obtained with a longer laser duration than the higher peak power density.
Aeroaocustic Numerical Analysis of the Vehicle Model
Authors: Hamiga Władysław Marek , Ciesielka Wojciech Bronisław
Published: December 2020

Understanding local phenomena connected with airflow around road vehicles allows to reduce the negative impact of transportation on the environment. This paper presents using numerical tools for Computational Fluid Dynamics (CFD) and Computational AeroAcoustic (CAA) calculation. As a model for simulation, simplified car geometry is used, which is known in the research community as an Ahmed body. The study is divided into two main parts: a validation process and a CAA analysis using the Ffowcs Williams–Hawkings (FW-H) analogy. Research is performed using k−ω Shear Stress Transport (SST) and the Large Eddy Simulation (LES) turbulence model. To compare results with other authors’ studies, three different comparison criteria are introduced: a drag coefficient for different velocities, characteristic flow structure, and velocity profiles. The CAA analysis is presented using colormaps and Fast Fourier Transformation (FFT). The methods used in this work allow visualizing the acoustic field around reference geometry and determining the frequency range for which the A-weighted sound pressure level is the highest.
Improving Air Quality for Operators of Mobile Machines in Underground Mines
Authors: Szczurek Andrzej , Maciejewska Monika , Przybyła Marcin , Szetelnicki Wacław
Published: December 2020

In underground mines, mobile mining equipment is critical for the production system. The microenvironment inside the mobile machine may cause exposure to strongly polluted mine air, which adversely affects the health and working performance of the operator. Harmful pollutants may access the cabin together with the ventilation air delivered from the machine’s surroundings. This work proposes a solution that is able to ensure that the air for the machine operator is of proper quality. The proposal emerged from an analysis of the compliance of cabins of mobile machines working underground in mines with occupational health and safety (H&S) standards. An analytical model of air quality in a well-mixed zone was utilized for this purpose. The cabin atmosphere was investigated with regard to the concentration of gaseous species in the surrounding air, the cabin ventilation rate, and human breathing parameters. The analysis showed that if currently available ventilation approaches are used, compliance with multiple H&S standards cannot be attained inside the cabin if standards are exceeded in the surroundings of the machine. The proposed solution overcomes this problem by combining elements that are already in place, i.e., ventilation, air-conditioning, and filtration with a personal supply of clean air. The concept is generic and may be adapted to various specific requirements.
Side Information Generation Scheme Based on Coefficient Matrix Improvement Model in Transform Domain Distributed Video Coding
Authors: Wang Wei , Chen Jianhua
Published: December 2020

In order to effectively improve the quality of side information in distributed video coding, we propose a side information generation scheme based on a coefficient matrix improvement model. The discrete cosine transform coefficient bands of the Wyner–Ziv frame at the encoder side are divided into entropy coding coefficient bands and distributed video coding coefficient bands, and then the coefficients of entropy coding coefficient bands are sampled, which are divided into sampled coefficients and unsampled coefficients. For sampled coefficients, an adaptive arithmetic encoder is used for lossless compression. For unsampled coefficients and the coefficients of distributed video coding coefficient bands, the low density parity check accumulate encoder is used to calculate the parity bits, which are stored in the buffer and transmitted in small amount upon decoder request. At the decoder side, the optical flow method is used to generate the initial side information, and the initial side information is improved according to the sampled coefficients by using the coefficient matrix improvement model. The experimental results demonstrate that the proposed side information generation scheme based on the coefficient matrix improvement model can effectively improve the quality of side information, and the quality of the generated side information is improved by about 0.2–0.4 dB, thereby improving the overall performance of the distributed video coding system.
The Meta-Dynamic Nature of Consciousness
Authors: Barnden John A.
Published: December 2020

How, if at all, consciousness can be part of the physical universe remains a baffling problem. This article outlines a new, developing philosophical theory of how it could do so, and offers a preliminary mathematical formulation of a physical grounding for key aspects of the theory. Because the philosophical side has radical elements, so does the physical-theory side. The philosophical side is radical, first, in proposing that the productivity or dynamism in the universe that many believe to be responsible for its systematic regularities is actually itself a physical constituent of the universe, along with more familiar entities. Indeed, it proposes that instances of dynamism can themselves take part in physical interactions with other entities, this interaction then being “meta-dynamism” (a type of meta-causation). Secondly, the theory is radical, and unique, in arguing that consciousness is necessarily partly constituted of meta-dynamic auto-sensitivity, in other words it must react via meta-dynamism to its own dynamism, and also in conjecturing that some specific form of this sensitivity is sufficient for and indeed constitutive of consciousness. The article proposes a way for physical laws to be modified to accommodate meta-dynamism, via the radical step of including elements that explicitly refer to dynamism itself. Additionally, laws become, explicitly, temporally non-local in referring directly to quantity values holding at times prior to a given instant of application of the law. The approach therefore implicitly brings in considerations about what information determines states. Because of the temporal non-locality, and also because of the deep connections between dynamism and time-flow, the approach also implicitly connects to the topic of entropy insofar as this is related to time.
Analysis of Symmetric Dual Switch Converter under High Switching Frequency Conditions
Authors: Tang Yu , Kong Dekai , Tong Haisheng
Published: December 2020

Electric vehicle batteries have the problem of low output voltage, so the application of a high-gain converter is a research hotspot. The symmetrical dual-switch high gain converter has the merits of simple structure, low voltage and current stress, and low EMI. Due to the deterioration of circuit performance caused by circuit parasitic parameters under high frequency operating conditions, the former analysis under low frequency condition cannot satisfy the requirements for performance evaluation. To clarify whether the symmetrical dual-switch high-gain converter can maintain its operating characteristics under high-frequency operating conditions, this paper establishes the converter model considering parasitic parameters, and deduces the sneak circuit modes at high frequency. The effects of parasitic parameters at high frequency on voltage gain, switch stress, and symmetrical operating are analyzed, which is beneficial for the selection and optimization of power devices. This paper believes that considering parasitic parameters may reduce the output gain of the symmetrical double-switch high-gain converter considering parasitic parameters under high frequency conditions, increase the switching stress, and affect the symmetry of the circuit operation when the parasitic parameter values are different. Finally, an experimental platform rated on 200 W with 200 kHz switching frequency is established, and experimental verification is given to verify the analysis.
Incineration Fly Ash and Its Treatment to Possible Utilization: A Review
Authors: Kanhar Altaf Hussain , Chen Shaoqing , Wang Fei
Published: December 2020

Incineration has gained popularity over landfill as a key solution for the reduction of massively increasing volumes of municipal solid waste (MSW) generation worldwide and in particular China. However, it is not the end solution. The disposal of the incineration residues, which are enriched with a wide range of heavy metals and soluble salts, has become a challenge for the environmental managers. The aim of this study was to review the increasing urbanization and its repercussion on waste generation in China; waste management options were compared for possible environmentally friendly considerations. Treatment techniques of incineration fly ash were discussed to determine the effectiveness of obtaining environmentally stable material, and, finally, possible applications of incineration fly ash for utilization were discussed based on identifying the processing suitability, performance and environmental impact of incineration fly ash for its applications.
Prevalence, Prevention, and Lifestyle Intervention of Gestational Diabetes Mellitus in China
Authors: Juan Juan , Yang Huixia
Published: December 2020

Gestational diabetes mellitus (GDM) has become an epidemic and has caused a tremendous healthy and economic burden in China, especially after the “two-child policy” put into effect on October 2015. The prevalence of GDM has continued to increase during the past few decades and is likely to see a further rise in the future. The public health impact of GDM is becoming more apparent in China and it might lead to the development of chronic non-communicable diseases in the long-term for both mothers and their children. Early identification of high-risk individuals could help to take preventive and intervention measures to reduce the risk of GDM and adverse perinatal outcomes. Therefore, a focus on prevention and intervention of GDM in China is of great importance. Lifestyle interventions, including dietary and physical exercise intervention, are effective and first-line preventive strategies for GDM prevention and intervention. The GDM One-day Care Clinic established in 2011, which educates GDM patients on the basic knowledge of GDM, dietary intervention, physical exercise, weight management, and blood glucose self-monitoring methods, sets a good model for group management of GDM and has been implemented throughout the hospitals as well as maternal and child health centers in China. The current review focus on the prevalence, risk factors, as well as prevention and lifestyle intervention of GDM in China for better understanding of the latest epidemiology of GDM in China and help to improve maternal and neonatal pregnancy outcomes and promote long-term health for women with GDM.
Radiological Assessment and Outcome of Local Disease Progression after Neoadjuvant Chemotherapy in Children and Adolescents with Localized Osteosarcoma
Authors: Fonseca Adriana , Ryan Anne L. , Gibson Paul , Hendershot Eleanor , Hopyan Sevan , Ranson Marilyn , Stimec Jennifer , Gupta Abha A.
Published: December 2020

Objective: We examined the interobserver reliability of local progressive disease (L-PD) determination using two major radiological response evaluation criteria systems (Response evaluation Criteria in Solid Tumors (RECIST) and the European and American Osteosarcoma Study (EURAMOS)) in patients diagnosed with localized osteosarcoma (OS). Additionally, we describe the outcomes of patients determined to experience L-PD. Materials and Methods: Forty-seven patients diagnosed with localized OS between 2000 and 2012 at our institution were identified. Paired magnetic resonance imaging of the primary tumor from diagnosis and post-neoadjuvant chemotherapy were blindly assessed by two experienced radiologists and determined L-PD as per RECIST and EURAMOS radiological criteria. Interobserver reliability was measured using the kappa statistic (κ). The Kaplan Meier method and log-rank test was used to assess differences between groups. Results: Of 47 patients (median age at diagnosis 12.9 years), 16 (34%) had L-PD (by RECIST or EURAMOS radiological definition). There was less agreement between the radiologists using EURAMOS radiological criteria for L-PD (80.9%, κ = 0.48) than with RECIST criteria (97.9%, κ = 0.87). Patients with radiologically defined L-PD had a 5-year progression-free survival (PFS) of 55.6%, compared to a 5 year-PFS of 82.7% in the group of patients without L-PD (n = 31) (Log rank p = 0.0185). Conclusions: The interobserver reliability of L-PD determination is higher using RECIST than EURAMOS. RECIST can be considered for response assessment in OS clinical trials. The presence of L-PD was associated with worse outcomes.
Comparative Study between Full-Endoscopic Discectomy and Microendoscopic Discectomy for the Treatment of Lumbar Disc Herniation
Authors: Fujita Muneyoshi , Kitagawa Tomoaki , Hirahata Masahiro , Inui Takahiro , Kawano Hirotaka , Iwai Hiroki , Inanami Hirohiko , Koga Hisashi
Published: December 2020

Background and objectives: Lumbar disc herniation (LDH) is a common disease in the meridian of life. Although surgical discectomy is commonly used to treat LDH, there are several different strategies. We compared the outcomes of uniportal full-endoscopic discectomy (FED) with those of microendoscopic discectomy (MED) in treating LDH. Materials and Methods: FED was performed using a 4.1-mm working channel endoscope, and MED was performed using a 16-mm diameter tubular retractor and endoscope. Data of patients with LDH treated with FED (n = 39) or MED (n = 27) by the single surgeon were retrospectively reviewed. Patient background information and operative data were collected. Pre- and postoperative low back and leg pain were evaluated using the numerical rating scale (NRS) score. Pre- and postoperative disc height index (DHI) values were calculated from plain radiographs, and the disc height loss was evaluated using the ratio (DHI ratio); Results: The median (interquartile range (IQR) Q25–75) operation times for FED and MED were 42 (33–61) and 43 (33–50) minutes, respectively. The median (IQR Q25–75) pre- and postoperative NRS scores for low back pain were 5 (2–7) and 1 (0–4), respectively, for FED and 6 (3–8) and 1 (0–2), respectively, for MED. The median (IQR Q25–75) pre- and postoperative NRS scores for leg pain were 7 (5–8) and 0 (0–2), respectively, for FED and 6 (5–8) and 0 (0–2), respectively, for MED. These data were not different between the FED and MED groups. The median (IQR Q25–75) DHI ratios of FED and MED were 0.94 (0.89–1.03) and 0.90 (0.79–0.95), respectively. The DHI ratio was significantly higher (p < 0.05) in the FED group than in the MED group, and there was less blood loss; Conclusions: The pain-relieving effect of FED in treating LDH was almost identical to that of MED. However, FED was superior to MED in preventing disc height loss, which is one of the indicators of postoperative disc degeneration.
TRANSPARASI DAN AKUNTABILITAS ANGGARAN PENDAPATAN DAN BELANJA DESA STUDI KASUS PADA DESA NGASEM KECAMATAN BATEALIT
Authors: Rohman Fatchur , Relandani Aci
Published: December 2020

This study aims to determine transparency and accountability in the implementation of the Village Income and Expenditure Budget in Ngasem Village, Batealit District, Jepara Regency. Because in Ngasem Village, Batealit Subdistrict, Jepara Regency, the Village Budget has always increased. In the Village Revenue and Expenditure Budget, priority is placed on village infrastructure. The data used in this study were obtained from data collection and carried out by direct observations in the field with interviews. The data analysis technique used in this study is a qualitative descriptive analysis with data processing methods with triangulation techniques. This interview technique was conducted directly with the Village Head, Village Secretary, Village Consultative Body, and Village Communities in Ngasem Village, Batealit District, Jepara Regency. The results of this study indicate that the Ngasem village has carried out transparency and accountability in the Village Budget of the 2018 budget year. In general the transparency and accountability in Ngasem Village has been in accordance with the provisions and has run well and smoothly.