bluuebunny/crossref_metadata_2025 huggingface dataset:
Interaksi sosial pada kalangan mahasiswi bercadar di lingkungan kampus
Authors: Praja Wina Nurhayati , Pandu Hyangsewu  , Isni Nurazizah
Published: November 2022

adar atau dalam bahasa arab disebut An-Niqab adalah sesuatu yang berguna untuk  menutupi seluruh wajah perempuan, kecuali kedua mata atau sesuatu yang tampak disekitar mata. Muslimah bercadar menjadi menarik untuk diteliti karena cara mereka menjalankan perintah Allah yang berbeda dengan muslimah pada umumnya. Sebagai manusia, wanita bercadar juga membutuhkan komunikasi, karena manusia adalah makhluk sosial yang berarti tidak dapat hidup tanpa orang lain. Dorongan atau motif sosial pada manusia, mendorong manusia mencari orang lain untuk mengadakan hubungan atau interaksi sehingga memungkinkan terjadi interaksi antara manusia satu mampu menampilkan diri sesuai dengan norma yang berlaku. Penelitian ini dilaksanakan di lingkungan kampus Universitas Pendidikan Indonesia, Bumi Siliwangi. Tujuan dari penelitian ini adalah untuk mengetahui dan menganalisa apa yang melatarbelakngi penggunaan cadar dikalangan Mahasisiwi dan bagaimana interaksi sosial mahasisiwi bercadar di Kampus UPI. Teknik penentuan sampel secara purposive sampling menetapkan jumlah subjek penelitian sebanyak 5 orang. Penulis menggunakan metode deskriptif kualitatif dengan instrumen data adalah observasi, wawancara dan studi kepustakaan.

Berdasarkan hasil dari penelitian, penulis menemukan adanya beberapa alasan yang melatar belakangi penggunaan cadar dikalangan mahasiswi, yaitu pemahaman dalam beragama, merubah diri untuk menjadi muslimah yang lebih baik, kemauan dalam diri sendiri untuk menggunakan cadar serta pengaruh dari teman dan lingkungan pergaulan yang baik juga. Interaksi Mahasiswi bercadar dengan lingkungan yang saat ini banyak bermunculan anggapan negatif dengan cadar. Penulis menemukan bahwa interaksi mahasiwi bercadar dengan teman satu kelompok atau teman perempuan yang sudah lama berinteraksi dengan mahasisiwi bercadar selalu mengalami kesulitan untuk mengenali temannya sendiri yang menggunakan cadar, kesulitan mengenali seseorang yang tidak terlihat wajah, interaksi mahasiswi bercadar dengan teman lawan jenis akan membatasi diri dan mengatur jarak atau hanya sekedar tegur sapa.
Pengaruh Model Pembelajaran Example Non-Examples Berbasis Project Citizen Terhadap Hasil Belajar Siswa
Authors: Hermuttaqien Bhakti Prima Findiga , Faradisa Eva , Manggau Arifin
Published: November 2022

Penelitian ini mempunyai tujuan untuk mengetahui ada tidaknya pengaruh signifikan penerapan model pembelajaran example non-examples berbasis project citizen terhadap hasil belajar siswa pada mata pelajaran PPKn kelas X di MA Al-Islam Bantur. Metode quasi experiment adalah metode yang digunakan oleh peneliti dengan jumlah siswa sebanyak 44. Pengambilan sampel menggunakan teknik clusterrandom dengan taraf kesalahan sebesar 5%. Adapun teknik pengumpulan data yang digunakan ialah tes dan angket. Sedangkan teknik analisis data yang digunakan ialah uji hipotesis yang terdiri dari uji T dan uji F. Berdasarkan pengujian pada uji T diketahui nilai Sig. (2-tailed) sebesar 0,000 < 0,05 yang artinya terdapat perbedaan signifikan rata-rata hasil belajar siswa. Sedangkan pada uji F diketahui nilai Sig. sebesar 0,02 < 0,05 yang artinya terdapat pengaruh signifikan penerapan model pembelajaran example non-examples berbasis project citizen terhadap hasil belajar siswa pada pelajaran PPKn kelas X di MA Al-Islam Bantur.
Political Geography of Indonesian Nationalism and Ethno Nationalism: A Reflective Study From Indonesian Generation Z
Authors: Warganegara Arizka
Published: November 2022

Debates on nationalism and ethno nationalism have intensely developed during the early decade of political reformation in Indonesia, that is, in the early 2000s. At that point, the demand for segregation from the Republic of Indonesia emerged from several districts, such as Riau and Papua. In this era, the sentiment to decentralised from the Republic of Indonesia has been well suppressed, particularly in some areas, Aceh included. On the other side of the coin, the process of welfare distribution is going well. It is supported by the balance of power between the central and local authorities, which has successfully reduced this sentiment. These observations were conducted through a qualitative method by conducting interviews with Generation Z, which were divided into two clusters: based on gender and the respondent’s status either as a student or a fresh graduate. This paper investigates the extent of understanding the nationalism and ethno nationalism among Generation Z in Indonesia. In-depth interviews were reflectively conducted in which the research participants were asked to answer some questions and share their views and understanding of this discussion. This study concludes that Generation Z Indonesian proved to have a comprehensive understanding of nationalism. They viewed that modules about nationalism should be officially included in elementary and high school curricula; thus, the students will be exposed and have a solid understanding of nationalism from an early age.
Investing in the Ummatic Cause: The Philanthropic Activities of ‘Abd Allāh ibn ‘Alawī al-’Aṭṭās (d. 1929)
Authors: Alatas Alwi , Chaedar Syed Mohammad
Published: November 2022

‘Abd Allāh ibn ‘Alawī al-’Aṭṭās (d. 1929) was born into a wealthy family and became a well-known Hadrami property-owner in Batavia (Jakarta). He lived in a dynamic era in the late 19th and early 20th centuries, which witnessed the awakening of the Hadrami community in the Netherlands East Indies or colonial Indonesia. As a strong supporter of Islamic reformism, he was heavily involved in disseminating the idea of progress and sponsoring a number of revivalist efforts, particularly in the field of education. His substantial financial contribution in this regard earned him a prominent reputation, although this was not always understood by his own community. However, his revivalist and philanthropic activities are rarely studied in depth. This article will discuss al-’Aṭṭās’ financial support toward the promotion of the ummatic cause in the Netherlands East Indies. The question of how his financial contributions were directed to improve the conditions of the ummah will be addressed thoroughly. This study uses the historical method and employs a number of primary sources that have not been widely used. It also utilizes research about charity and philanthropy to enrich the discussion. This study will, hopefully, broaden our understanding of the role of philanthropic activities in an effort to infuse revivalism in the Muslim society through one of its main proponents in the Netherlands East Indies.
Hashemites’ Custodianship of Jerusalem Islamic Holy Sites
Authors: Aljedayah Karimah Suleiman , Alatoom Nabeel Ali , Jraiban Mohammad Ibrahim Abu , Abumelhim Mohammad Husni , Abu-Melhim Abdel-Rahman , Rababah Mahmoud Ali
Published: November 2022

This study aims to demonstrate the Jordanian Hashemites’ custodianship of the Islamic sanctities in Jerusalem and their role in preserving its Islamic identity from the attempts of Judaization exposed by the Israeli State. The reigns of his Majesty King Abdullah I and King Hussein (kings of the Hashemite Kingdom of Jordan) were taken as a model for the study, by presenting the most prominent services provided to Al-Aqsa Mosque and the Dome of the Rock in Jerusalem, such as restoration, renovation, rebuilding, and providing financial and non-financial support to these sanctities. The study concludes that the Hashemites’ custodianship of the Islamic sanctities in Jerusalem was not only connected with the Jordanian-Palestinian relations, but also came out of the Hashemites’ faith in their religious and national duty to preserve Jerusalem identity and stand in the face of any attempt to Judaize, control and divide Jerusalem. This custody began since the era of Sharif Hussein bin Ali and it has continued to the present time.
Some Subclasses of Univalent and Bi-Univalent Functions Related to K-Fibonacci Numbers and Modified Sigmoid Function
Authors: Rashid Amal Madhi , Juma Abdul Rahman S.
Published: November 2022

يهتم هذا البحث بفئات فرعية معينة من الدوال احادية التكافؤ وثنائية التكافؤ فيما يتعلق بمنحنيات تشبه الصدفة المرتبطة بأرقام فيبوناتشي k تتضمن دالة التنشيط السيني المعدلة θ(t)=2/(1+e^(-t) ) ,t ≥0في قرص الوحدة|z|<1. تقديرات المعاملات الاولية |c_2 | , |c_3 | تم التحقق من عدم المساواة Fekete-Szego ̈ ومحدد هانكل الثاني للدوال في فئاتنا.
Design and study the effect of inner bore diameter on the magnetic and optical properties of the unipolar lens
Authors: AL- Janan Mohammed.K.A. , AL-Salih R.Y. J.
Published: November 2022

تم وضع عدة تصاميم لعدسات مغناطيسية أحادية القطب اعتماداً على تغيير عرض التجويف الداخلي وتثبيت المعالم الهندسية الأخرى للعدسة من اجل اجراء دراسة امكانية تحسين أداء العدسة المغناطيسية أحادية القطب. تم اجراء دراسة لكل تصميم تتضمن دراسة تمغنط العدسة وحساب المجال المغناطيسي المحوري إضافة الى كثافة الفيض المغناطيسي باستخدام طريقة العناصر المتناهية (FEM) لبرنامج تشغيل العدسات الالكترونية المغناطيسية (MELOP) النسخة الاولى عند ثلاث قيم مختلفة لكثافة التيار (6،4،2 (A/mm2¸ وتم الحصول على اوضح القيم والسلوك عندما كانت كثافة التيار بمقدار (2 A/mm2). وجد ان أفضل خواص تمغنط واعلى قيمة لكثافة الفيض المغناطيسي واقل قيمة لعرض نطاق شدة المجال المغناطيسي المحوري تم الحصول عليها عندما كان عرض التجويف الداخلي بمقدار(mm55)، وكما تم دراسة تأثير كثافة التيار على الخصائص البصرية، حيث لوحظ بان قيم الزيوغ اللونية والكروية قلت بشكل ملحوظ لذلك تم اختيار هذه العدسة كأفضل تصميم من بين التصاميم المقترحة للعدسة الأحادية القطب.
In vitro isolation and expansion of neural stem cells NSCs
Authors: Mohammad Maeda H. , Almzaien Aous Kahtan , Al-Joubory Ahmad A. , Al-Shammari Ahmed M. , Ahmed Ayser A. , Shaker Hiba K. , Abedalsattar Aseel K.
Published: November 2022

الخلايا الجذعية العصبية (NSCs) هي خلايا مولدة لديها القدرة على التجديد الذاتي والقدرة على التمايز إلى خلايا عصبية نجمية astrocytes ، وخلايا عصبية قليلة التفرع  oligodendrocyte، و خلايا عصبية neuron . في هذه الدراسة تم عزل الخلايا الجذعية في المختبر ، وتنميتها في المختبر ، وتحديد هويتها ، ومن ثم انتاج الخلايا الجذعية العصبية وحفظها بالنيتروجين السائل كمصدر خلايا ناجح لاستخدامها لاحقا في التجارب العلاجية. تم استخدام نخاع عظم الفأر كمصدر للخلايا الجذعية العصبية في هذه الدراسة. أظهرت نتائج دراسة الشكل المظهري والكيمياء المناعية للخلايا المعزولة أنه يمكننا إنتاج الخلايا الجذعية العصبية NSCs بنجاح والحفاظ على تجديدها الذاتي وتشكيلها بنجاح في الغلاف العصبي ولتمريرات متعددة. تم الحفاظ على الشكل المظهري للخلايا الناتجة وتخزينها في النيتروجين السائل لتكون مصدرا جاهزا للاستخدام في التجارب كنموذج لعلاج الاضطرابات العصبية.
Exploring computational techniques for simulating residual stresses for thin wall multi-joint hexagon configurations for a laser directed energy deposition process
Authors: Mohajernia Bita , Urbanic Jill
Published: November 2022

Laser cladding is a directed energy deposition process, and can lead to high residual stresses, which can compromise the quality of the specimen. As a result, it is crucial to accurately predict and investigate the residual stress distribution in cladded parts and understand the mechanism of formation. In this study a thermo-mechanical metallurgical simulation model of the laser cladding process was developed for three different path strategies with respect to the deposition sequence and direction for a thin wall hexagon with inner junctions to investigate the formation of residual stress. The study was performed for single and multilayer scenarios. Two types of computational techniques, the detailed transient approach and the imposed thermal cycle approach, was performed and comparisons conducted. Consistent results were observed when comparing the resultant stress patterns for the single layer; subsequently, the imposed thermal cycle method was applied for the five layer models. A preheat scenario is explored. This reduced the computational cost significantly, but the stress patterns were not similar. This indicates that building up worn regions at the top of a thin walled component, such as a roll die, needs to be investigated further as unique issues have been highlighted. The differences between the implemented computational techniques are described as well as the advantages and disadvantages of each. Knowledge obtained from these case studies provides a foundation for efficient and rapid optimization of laser cladding processes, with the aim of minimizing residual stress in both simple and complex laser cladding structures.
Age-specific information resources to address the needs of young people with stroke: a scoping review protocol
Authors: Gopaul Urvashy , Charalambous Marina , Thilarajah Shamala , Kwah Li Khim , Chapman Sherita , Bayley Mark , Demers Marika
Published: November 2022

Background and aims Young people with stroke (YPwS) persistently experience challenges with disability, social reintegration, employment and financial stability to provide for themselves and their families. The aims of this scoping review are to: 1) identify and collate information resources for YPwS and evidence-based self-managements programs and,2) identify gaps in age-specific resources available for YPwS after traditional rehabilitation services have ended and/or who are returning to live in the community.
Methods We will include both qualitative and quantitative studies, including all study designs. Participants will be community-dwelling adults aged between 18–65 years with a clinical diagnosis of stroke. We will include information resources and evidence-based self-managements programs for YPwS. Search terms will include stroke, young people, community-dwelling. We will search electronic databases such as MEDLINE. The reference lists of included studies, systematic reviews and stroke guidelines and stroke specific websites will also be searched. We will also contact Stroke Support Organizations and international/national allied health professional organisations to gather information resources about YPwS. We will also conduct a comprehensive environmental scan of additional resources using the search engine Google. The titles, abstracts, full-text articles and contents of the resources identified by the search will be assessed against the inclusion and exclusion criteria to identify potentially relevant resources.
Results and Conclusions Existing resources and self-management programs will be collated and categorized according to the type of needs addressed such as physical, emotions, activities of daily living, information, relationships and social needs as well as the key gaps identified.
Dysregulation of CELF4 splicing factor in pancreatic neuroendocrine tumors enhances aggressiveness and alters mTOR pathway and everolimus response
Authors: Castano Justo P , Alors-Perez Emilia , Pedraza-Arevalo Sergio , Agraz-Doblas Antonio , Blázquez-Encinas Ricardo , García-Vioque Víctor , M Yubero-Serrano Elena , Sánchez-Frías Marina E. , Serrano-Blanch Raquel , Gálvez-Moreno María Ángeles , Gracia-Navarro Francisco , Gahete Manuel D. , Arjona-Sánchez Álvaro , Luque Raúl M. , Ibáñez-Costa Alejandro
Published: November 2022

Background
Pancreatic neuroendocrine tumors (PanNETs) comprise a highly heterogeneous group of rare tumors, with growing incidence. Recent molecular analyses provided a precise picture of their genomic and epigenomic landscape. Splicing dysregulation is increasingly regarded as a novel cancer hallmark influencing all key tumor features. In this context, we have previously demonstrated that splicing machinery is markedly dysregulated in various cancers, including PanNETs. Here, we aimed to elucidate the molecular features and functional implications pertaining to one of the most altered splicing factors in PanNETs, CELF4.
Methods
CELF4 expression levels were determined in a cohort of 20 PanNET patients, comparing tumor and non-tumoral adjacent tissue, used as reference. RNA-Seq dataset was analyzed to explore CELF4-linked interrelations among clinical features, gene expression, and splicing event profiles. Two PanNET model cell lines, BON-1 and QGP-1, were employed to assess CELF4 function in vitro, including a detailed mTOR phospho-antibody array, and in vivo in BON-1-xenografted mice.
Results
PanNETs display markedly upregulated CELF4 expression levels, which closely associate with relevant malignancy features, specific expression of key tumor players (e.g., TP53), and distinct splicing event profiles. Functionally, modulation of CELF4 expression influenced PanNET cell lines proliferation in vitro, while CELF4 silencing in vivo reduced BON-1 xenograft tumor growth. Interestingly, CELF4 silencing enhanced the antiproliferative effect of the mTOR inhibitor everolimus in PanNET cells. Furthermore, detailed analysis of this core signaling pathway under CELF4 silencing showed changes in phosphorylation of the molecular components of the mTOR pathway.
Conclusions
Our results demonstrate that the splicing factor CELF4 is dysregulated in PanNETs, where it can influence tumor development and aggressiveness, likely by modulating mTOR pathway. These novel findings invite to explore in further detail this splicing factor as actionable therapeutic target in PanNETs.
Three-Dimensional Digital Visualization System Assisted Vitrectomy for Infectious Endophthalmitis
Authors: Guan Lina , Chen Jiayu , Zhang Zhengpei , Ji Sujuan , Liu Yalu , Li Suyan , Liu Haiyang
Published: November 2022

Purpose: To present our experiences and surgical efficacy of the three-dimensional(3D)visualization system in endophthalmitis vitrectomy.
Methods: 11 cases of endophthalmitis had undergone NGENUITY® 3D Visualization System assisted23-gauge transconjunctival vitrectomy, by a same surgeon at Xuzhou First People’s Hospital between 1 March 2020 and 31 March 2022. The efficacy of vitrectomy , best-corrected visual acuity (BCVA), intraoperative illumination, and comfort of the surgeon was investigated.
Results: The vitrectomy was finished in all cases with lower intensity of intraoperative illumination on the 3D visualizationsystem without any complications attributed to the visualization system, and none of them were abdicated or converted to the conventional microscope. All of the patients were cured after operation combined with antibiotics therapy, consistent with significantly improved BCVA(t = 4.039, p = 0.0024).
Conclusion: 3D visualizationsystem with advantages of clearer intraoperation visualization and optimized ergonomic design, is suitable for a variety of vitreoretinal surgery with good safety and efficacy, especially in the complicated and long-lasting endophthalmitis surgeries.
Case-matched radiological and clinical outcome evaluation of interlaminar versus microsurgical decompression of lumbar spinal stenosis​
Authors: Saravi Babak , Ülkümen Sara , Lang Gernot , Couillard-Despres Sebastien , Hassel Frank
Published: November 2022

Purpose
Endoscopic spine surgery is a globally expanding technique advocated as less invasive for spinal stenosis treatment compared to the microsurgical approach. However, evidence on the efficiency of interlaminar full-endoscopic decompression (FED) vs. conventional microsurgical decompression (MSD) in patients with lumbar spinal stenosis is still scarce. We conducted a case-matched comparison for treatment success with consideration of clinical, laboratory, and radiologic predictors.
Methods
We included 88 consecutive patients (FED: 36/88, 40.9%; MSD: 52/88, 59.1%) presenting with lumbar central spinal stenosis. Surgery-related (operation time, complications, length of stay (LOS), American Society of Anesthesiologists Physical Status (ASA) score, C-reactive protein (CRP), white blood cell count, side of approach (unilateral/bilateral), patient-related outcome measures (PROMs) (Oswestry Disability Index (ODI), Numeric Rating Scale of Pain (NRS; leg-, back pain), EuroQol questionnaire (eQ-5D), Core Outcome Measures Index (COMI)), and radiological (dural sack cross-sectional area, Schizas score (SC), left and right lateral recess heights, and facet angles, respectively) parameters were extracted at different time points up to 1-year follow-up. The relationship of PROMs was analyzed using Spearman’s rank correlation. Surgery-related outcome parameters were correlated with patient-centered and radiological outcomes utilizing a regression model to determine predictors for propensity score matching.
Results
Complication (most often residual sensorimotor deficits and restenosis due to hematoma) rates were higher in the FED (33.3%) than MSD (13.5%) group (p < 0.05), while all complications in the FED group were observed within the first 20 FED patients. Operation time was higher in the FED, whereas LOS was higher in the MSD group. Age, SC, CRP revealed significant associations with PROMs. We did not observe significant differences in the endoscopic vs. microsurgical group in PROMs. The correlation between ODI and COMI was significantly high, and both were inversely correlated with eQ-5D, whereas the correlations of these PROMs with NRS findings were less pronounced.
Conclusions
Endoscopic treatment of lumbar spinal stenosis was similarly successful as the conventional microsurgical approach. Although FED was associated with higher complication rates in our single-center study experience, the distribution of complications indicated surgical learning curves to be the main factor of these findings. Future long-term prospective studies considering the surgical learning curve are warranted for reliable comparisons of these techniques.
Carbon disulfide removal from gasoline fraction using zinc-carbon composite synthesized using microwave-assisted homogenous precipitation.
Authors: Sakr Ayat A.-E. , Amr Noran , Bakry Mohamed , El-Azab Waleed , Ebiad Mohamed
Published: November 2022

Carbon disulfide (CS2) is one of the sulfur components that are naturally present in petroleum fractions. Its presence causes corrosion issues in the fuel facilities and deactivates the catalysts in the petrochemical processes. It is a hazardous component that negatively impacts the environment and public health due to its toxicity. This study used zinc-carbon (ZC) composite as a CS2 adsorbent from the gasoline fraction model component. The carbon is derived from date stone biomass. The ZC composite was prepared via a homogenous precipitation process by urea hydrolysis. The physicochemical properties of the prepared adsorbent are characterized using different techniques. The results confirm the loading of zinc oxide/ hydroxide carbonate and urea derived species on the carbon surface. The results were compared by the parent samples, raw carbon, and zinc hydroxide prepared by conventional and homogeneous precipitation.
The CS2 adsorption process was performed using a batch system at atmospheric pressure. The effects of adsorbent dosage and adsorption temperatures have been examined. The results indicate that ZC has the highest CS2 adsorption capacity (124.3 mg.g-1 at 30˚C) compared to the parent adsorbents and the previously reported data. The kinetics and thermodynamic calculations results indicate the spontaneity feasibility of the CS2 adsorption process.
An experimental study of mitigation of fire hazards of flash powder by adding Mono Ammonium Phosphate as a solid inertant for explosive waste disposal in firework industries
Authors: Arumugachamy Azhagurajan , Pandian Prakash Lakshmana
Published: November 2022

In firework industries, flash powder which is a chemical mixture is used for crackers manufacturing. Flash powder is a highly inflammable chemical mixture. It consists of Aluminium, Sulphur, and Potassium Nitrate. In the manufacturing, daily wastes are collected and burned in the open pit. This leads not only to air pollution but also causes many accidents. There seems to exist no satisfactory method of waste disposal that is suitable for explosive waste. Here, the author has suggested a novel method according to which the explosives can be converted into an inert substance by adding solid inertants and then can be safely disposed by land fill. A solid inertant mono-ammonium phosphate (MAP) chemical was utilized to lower the flash powder's sensitivity. MAP is a fire extinguishing agent which can also be used as a fertilizer for the soil. In this work, various tests like layer ignition temperature, friction and impact sensitivity, plant growth test and decomposition test were conducted for the MAP mixed flash powder. From the various tests, it has been concluded that 50% MAP mixed flash powder would be safe to handle and that 75% mixed flash powder does not decompose at all weather conditions. After land filling, soil is not polluted, and it could be used for agriculture purpose.
Ulinastatin ameliorates acute pancreatitis and associated intestinal and pulmonary injury by inhibiting NLRP3 inflammatory vesicles and affecting intestinal flora composition and metabolic pathways in rat
Authors: Xu Lin , Zhang Jinglan , Guo Junqi , Liu Chao , Yang Jing , Yu Yang , Liu Wen , Zhu Guochao
Published: November 2022

Background
Acute pancreatitis (AP) is a common and potentially fatal disease, and severe AP can lead to systemic or local complications, such as enterocolitis and lung injury. The purpose of this study was to explore the mechanism of ulinastatin inhibition of Acute pancreatitis (AP) and its complications, and the effect of ulinastatin on intestinal flora and intestinal metabolism in AP rats.
Methods
Establish AP rat model, and detect serum lipase and α- Amylase activity content, dry/wet specific gravity of pancreas, and plasma Content of IL-1 β, TNF- α, IL-6, IL-10 and D-lactic acid, Histopathology was used to analyze the pathological changes of pancreas, intestine and lung, and Western blot was used to detect NLRP3, Caspase 1 and IL-1 β expression in pancreas, analysis of the protection of ulinastatin on intestinal mucosal barrier, analysis of the impact of ulinastatin on species richness and biodiversity of intestinal flora in rats with acute pancreatitis, analysis of the impact of ulinastatin on intestinal metabolites in AP rats, and analysis of the correlation between common differential metabolites and dominant flora.
Results
The results indicate that ulinastatin inhibited the conversion of pro-IL-1β to mature IL-1β by inhibiting NLRP3 and Caspase 1, the constituent proteins of NLRP3 inflammatory vesicles, and thereby suppressed the release of pancreatic edema, neutrophil infiltration and inflammatory cytokines IL-1β, TNF-α and IL-6 induced by AP, and increased the level of anti-inflammatory factor IL-10. This inhibitory mechanism of inflammatory response of ulinastatin also has the same inhibitory effect on enteritis and pneumonia caused by AP. Ulinastatin protects the intestinal mucosal barrier by increasing the levels of tight link proteins (Claudin, Occludin and ZO-1) in AP rats. Ulinastatin shifted the dominant intestinal flora of AP rats to Bacteroidaceae-Enterobacteriaceae, which may shift the fatty acid acquisition pathway in the mouse intestine from synthetic fatty acids to catabolic long- and medium-chain fatty acids. This regulatory effect on intestinal flora would further affect metabolic pathways such as amino acid metabolism and vitamin B6 metabolism in AP rats.
Conclusions
Ulinastatin can inhibit NLRP3 inflammatory vesicles and related proteins, and it can also affect intestinal flora composition and metabolic pathways in rats to improve acute pancreatitis and related intestinal and lung injuries
Hypermethylation of ACADVL is involved in high-intensity interval training-associated reduction of cardiac fibrosis in heart failure patients
Authors: Hsu Chih-Chin , Wang Jong-Shyan , Shyu Yu-Chiau , Fu Tieh-Cheng , Juan Yu-Hsiang , Yuan Shin-Sheng , Wang Chao-Hung , Yeh Chi-Hsiao , Liao Po-Cheng , Wu Hsin-Yi , Hsu Pang-Hung
Published: November 2022

Background

Emerging evidence suggests that DNA methylation can be affected by physical activities and is associated with cardiac fibrosis. The translational research examined the implications of DNA methylation presentations behind high-intensity interval training (HIIT) effects on cardiac fibrosis in patients with heart failure (HF).

Methods

Twelve HF patients were included and received cardiovascular magnetic resonance imaging with late gadolinium enhancement for cardiac fibrosis severity and cardiopulmonary exercise test for peak oxygen consumption (⩒O2peak). Afterwards, they underwent 36 sessions of HIIT at alternating 80% and 40% of ⩒O2peak for 30 min per session in 3–4 months. Human serum from 11 participants, linking cell biology to clinical presentations, was used to investigate exercise effects on cardiac fibrosis. Primary human cardiac fibroblasts (HCFs) incubated in patient serum for cell behaviors, proteomics (n = 6) and DNA methylation profiling (n = 3) were performed. All measurements were followed after completing HIIT.

Results

An increase of ⩒O2peak along with decreased b-type natriuretic peptide was observed after HIIT. Significantly decreased left ventricle (LV) myocardium fibrosis by 8–12% at middle and apical myocardial segments, decreased LV volume, and increased LV ejection fraction were identified after HIIT. 49 in 1222 identified proteins were significantly involved in the HIIT-induced altered HCF activities. A significant hypermethylation on acyl-CoA dehydrogenase very long chain (ACADVL) gene was identified. Downstream caspases-mediated actin disassembly and cell death pathway were activated after HIIT.

Conclusions

HIIT is associated with hypermethylation of ACADVL to impede HCF activities. This exercise-associated epigenetic reprogramming may contribute to reduce cardiac fibrosis and furthermore, promotes cardiorespiratory fitness in HF patients.

Trial registration

: NCT04038723. Registered 31 July 2019, https://clinicaltrials.gov/ct2/show/NCT04038723.
Identifying the Role of NUDCD1 in Human Tumors from Clinical and Molecular Mechanisms: A Study Based On Comprehensive Bioinformatics and Experimental Validation
Authors: Han Bin , He Jinsong , Li Yuanting , Chen Qing , Yuan Min , Zeng Xi , Zeng Yan , He Meibo , Feng Dan , Ma Daiyuan
Published: November 2022

Purpose
Cancer is a major public health condition characterized by high morbidity and mortality. NUDCD1 (NudC domain-containing 1) is abnormally activated in multiple tumors and has been identified as a cancer antigen. But there is still no pan-cancer analysis available for NUDCD1 in human cancers.
Methods
The role of NUDCD1 across multiple tumors was explored using data from the public databases including HPA, TCGA, GEO, GTEx, TIMER2, TISIDB, UALCAN, GEPIA2, cBioPortal, GSCA and so on. Molecular experiments (e.g., quantitative real-time PCR, immunohistochemistry and western blot) were conducted to validate the expression and biological function of NUDCD1 in STAD tissues and cells.
Results
NUDCD1 was highly expressed in most tumors and its levels were associated with the prognosis of cancer patients. Multiple genetic and epigenetic features of NUDCD1 exist in different cancers such as uterine corpus endometrial carcinoma or kidney renal papillary cell carcinoma. NUDCD1 was associated with expression levels of recognized immune checkpoints (anti-CTLA-4) and immune infiltrates (e.g., CD4 + and CD8 + T cells) in some cancers. Moreover, NUDCD1 correlated with the CTRP and GDSC drug sensitivity and acted as a link between chemicals and cancers. The functional mechanisms of NUDCD1 included RNA metabolism- and protein processing-associated functions. Importantly, NUDCD1-related genes were enriched in several tumors (e.g., COAD, STAD and ESCA) and affected apoptosis, cell cycle and DNA damage cancer-related pathways. Furthermore, expression, mutation and copy number variations for the gene sets were also associated with prognosis. At last, we experimentally validated the overexpression of NUDCD1 in STAD clinical samples and determined its contribution to STAD in vitro and in vivo.
Conclusions
NUDCD1 was involved in diverse biological processes and it influenced the occurrence and development of cancers through diverse underlying mechanism. This first pan-cancer analysis for NUDCD1 provides a comprehensive understanding about its roles across various cancer types, especially in STAD.
Optical coherence tomography in patients with Wilson’s disease: A single center, prospective study
Authors: Ning Wei Qin , Lyu Chun Xiao , Diao Sheng-Peng , Huang Ye-Qing , Liu Ai-Qun , Yu Qing-Yun , Hong Ming-Fan , Peng Zhong-Xing , Zhou ZhiHua
Published: November 2022

Background: Morphological changes of retina in patients with Wilson’s disease
(WD) can be found by optical coherence tomography (OCT), and such changes have significant differences between neurological forms（NWD ) and hepatic forms (HWD) of WD. We aimed to evaluate the relationship between morphological parameters of retina and brain magnetic resonance imaging (MRI) changes, course of disease, type of disease and sexuality in WD. This is a single center, prospective study including forty-six WD patients and forty healthy controls (HC). We employed theGlobal Assessment Scale (GAS) to assess the the neurological sign of WD patients.
Results: NWD had thinner superior parafovea zone (108.07±6.89 um vs. 114.40±5.54 um, p<0.01), temporal parafovea zone (97.17±6.65 um vs. 103.60±4.53 um, p<0.01), inferior parafovea zone (108.114±7.65 um vs. 114.93±5.84 um, p<0.01) nasal parafovea zone (105.53±8.01 um vs. 112.10±5.44 um, p<0.01) in inner retina thickness than HWD. The course of disease influenced the retina thickness, male patients had thinner inner retina thickness than female patients.
Conclusion: Our results demonstrated that WD had thinner inner retina thickness than HC and NWD had thinner inner retina thickness than HWD. We spectualted the thickness of inner retina layer may a potential useful biomarker for NWD.
Inequalities in access to NHS primary care dental services in Scotland during the COVID-19 pandemic
Authors: Aminu Abodunrin , McMahon Alex , Clark Claire , Sherriff Andrea , Buchanan Caroline , Watling Chris , Mahmoud Ahmed , Culshaw Shauna , MacKay William , Gorman Megan , Braid Raymond , Edwards Maura , Conway David
Published: November 2022

Introduction: This study aims to quantify the impact of the COVID-19 pandemic on access and inequalities in primary care dental services among children and adults in Scotland.
Methods: Access was measured as any NHS Scotland primary care dental contacts derived from administrative data from January 2019 to May 2022, linked to the area-based Scottish Index of Multiple Deprivation (SIMD) for children and adults, and related to population denominator estimates from National Record Scotland. Inequalities for pre-pandemic (January 2019 – January 2020) and recent (December 2021 – February 2022 and March 2022 – May 2022) periods for both children and adults were calculated and compared using the slope index of inequality (SII) and relative index of inequality (RII).
Results: Following the first lockdown (March 2020) there was a dramatic fall to near zero dental contacts, followed by a slow recovery to 64.8% of pre-pandemic levels by May 2022. There was initial widening of relative inequalities in dental contacts in early 2022, which, more recently, had begun to return to pre-pandemic levels.
Conclusion: COVID-19 has had a major impact on access to NHS primary dental care, and while inequalities in access are apparent as services recover from lockdown, these inequalities are not a new phenomenon.
Changing Patterns in temporal trends of diabetes mortality in China from 1987-2020: a rapid growth among rural populations
Authors: chen chen , Su Binbin , Zhao Yihao , Wu Yu , Zhong Pangliang , Zheng Xiaoying
Published: November 2022

Diabetes mellitus (DM) is a public health challenge in China. Quantifying changes in DM mortality is of great significance, but related evidence of Chinese older adults is scarce. This study conducted a comprehensive analysis of China’s DM mortality changing trajectory. The data from 1987 to 2020 was derived from the National Health Commission of China. Joinpoint analysis and age-period-cohort (APC) model were used to calculate the annual change and the effects of age, period and cohort on DM mortality. The changing trajectory from 1987 to 2020 showed an upward trend, especially among rural groups, and the gap between rural-urban narrowed. Joinpoint analysis showed a consistent upward trend in rural areas (4.9%, 95%CI: 4.0%-5.7%). However, the urban showed an upward trend from 1987–1998 (5.7%) and a slow downward trend from 1998 to 2020 (-1.0%). The APC model showed that the reporting rate increased with age in urban and rural areas. For the effect of time period and cohort, as they proceeded, the rural showed a consistent and significant upward trend, but the trends in urban remained relatively stable. The DM mortality in China has shown an overall upward trend over the past three decades, especially in rural areas. Although the gap between urban-rural has been further reduced, the burden of the oldest-older has risen significantly regardless of areas. It is time to give priority attention to the high mortality of DM among females, the elderly and rural areas, and the government should take measures to curb this trend.
Anaphylactic Shock Following Spray of “Gulliver” (Azimsulfuron), A Herbicide Belong To Sulfonylurea Group; A Case Report
Authors: Lankarathna Aluth Patabedige Chamila Pradeep , Medagama Arjuna
Published: November 2022

Background
Anaphylactic shock is a life threatening emergency encountered frequently with rising incidence worldwide. It is an extreme end of a spectrum of allergic reactions commonly present following multiple triggers, including food, medications, insect stings, dyes, etc. Patients usually present with a combination of dermatological, cardiovascular, respiratory and gastrointestinal symptoms. This is a rarely encountered case of anaphylactic shock following the spraying of sulphur containing herbicide called “Gulliver” (Azimsulfuron).
Case presentation
This 58-year-old farmer with previously uneventful past medical history was admitted to the emergency unit with an episode of faintishness and urticarial rash while working in a paddy field. There was no shortness of breath, wheezing or angioedema. He revealed that he had sprayed an herbicide call ‘Gulliver’ (Azimsulfuron, a sulphur containing herbicide) in the morning for 3 to 4 hours without using proper protective equipments and cloths. He denied any insect bites or stings. On admission, he was in shock with blood pressure 60/48mmHg, pulse rate 84 beats per minute with bounding pulses and warm peripheries. He was managed as anaphylactic shock according to the national health guideline and hemodynemically stabilized within 20 minutes of initial resuscitation including intramuscular adrenaline.
Conclusions
It is a well-known fact that a significant proportion of the general population has an allergy to sulphur containing medications. However, this was a rare encounter, detected with a high degree of suspicion. In Sri Lanka, a survey in 2016/2017 revealed 27 percent of the total labour force was engaged in the agricultural sector. We emphasize the importance of consideration of allergy and anaphylaxis in similar presentation in an agricultural based country like Sri Lanka.
Biochemical responses in cotton as diagnostic parameter for resistance against cotton leaf curl virus (CLCuV)
Authors: Chauhan Prashant , Mehta Naresh , Chauhan R. S. , Kumar Abhishek , Singh Harbinder
Published: November 2022

Cotton (Gossypium hirsutum L) is one of the most important staple fibrous crops cultivated in India and globally. Its production and quality are greatly hampered by cotton leaf curl disease (CLCuD) caused by cotton leaf curl virus (CLCuV). Therefore, the aim of present study was to investigate biochemical resistance responses in different cotton varieties against CLCuV. Four commercial cotton varieties with susceptible (HS 6 and RCH-134 BG-II) and resistant (HS 1236 and Bunty) response were used to analyse the role of primary (sugar, protein and chlorophyll) and secondary (gossypol, phenol and tannin) biochemical compounds produced by the plants against infection of CLCuV. The resistant cultivars with increased activity of protein, phenol and tannin exhibited as biochemical barriers against CLCuV infection imparting the resistance in cotton cultivars. Whereas, other biochemical compounds including chlorophyll, sugar and gossypol did not show significant role in resistance against CLCuV. Nevertheless, these compounds virtually associated with basic physiological and metabolic mechanisms of cotton plants. Among the primary biochemical compounds, only protein activity proposed as first line of defence in cotton against CLCuV. The secondary level of defence line in resistance exhibited the activity of secondary biochemical compounds phenol and tannins which exhibited significant increase in their level while imparting resistance against CLCuV in cotton.
Assessing Secular Trends in HIV Rapid Diagnostic Test Uptake and Positivity in Northeast Iran, a country in MENA region, and Recommending Gender-Specific Health Policies to Improve.
Authors: Yousefli Zahra , Maharlouei Najmeh , Moghaddam Maliheh Dadgar , Hosseinpour Ali Mohammad , Ghiami Roohollah
Published: November 2022

Background
Iran is among the first three countries in MENA region where two-thirds of new HIV infections are reported. The HIV rapid diagnostic testing (HIV-RDT) program, established for early detection of HIV infection, is evaluated in Mashhad, a metropolitan area, and 14 cities in Khorasan-Razavi Province, Iran.
Methods
In this cross-sectional study, de-identified records of HIV-RDT clients between 2017 and 2021 were extracted from the electronic health information system of 122 testing sites. Descriptive, bivariate and multivariate logistic regression analyses were performed to identify the factors associated with HIV-RDT uptake and risks and drivers of HIV-RDT positivity.
Results
Conducting 66546 HIV-RDTs among clients with a mean age of 30.31 years, 63% female, 75.2% married, and 78.5% diploma or below, yielded 312 (0.47%) positive results. Prenatal care and suspicious heterosexual intercourse were the most frequent reasons for taking HIV-RDT among women and men, respectively (76% and 61.2%). Test uptake was relatively low among men and the unmarried population. Male gender (odd ratio (OR) = 9.5), higher age at the time of testing (OR = 1.03), widowhood (OR = 3.98), education levels of high school diploma or below (OR = 2.35), and unemployment status (OR = 2.91) were significant sociodemographic predictors of positive HIV-RDT (P-value < 0.05). Furthermore, gender-stratified multivariate analyses revealed unsanitary dental procedures (OR = 15.7), mother-to-child transmission (MTCT)(OR = 29.95), drug injection (OR = 8.21), and male homosexuality (OR = 19.38) as behavioral predictors of test positivity among males (P-value < 0.05). Whilst substance abuse (not only injection) (OR = 12.15), MTCT (OR = 13.54), and being an occupational trainee (OR = 15.08) were predictors among females (P-value < 0.05).
Conclusion
The study provides significant targets to implement innovative strategies to increase test uptake and yield in the region, and also suggests gender-specific strategies, according to the sociodemographic and behavioral risks, for HIV primary prevention and early detection.
Role of toll-like receptors on placental derived mesenchymal stem cells and their protection from natural killer cells
Authors: Chen Gecai , Wang Meixiang , Yue Aihuan , Ruan Zhongbao , Zhu Li , Tang Chengchun
Published: November 2022

NK cells are major effector cells of the innate immunity. It is not clear that how MSCs can escape allogeneic NK cells killing and survival. The study included human placenta from women who were healthy and had full-term naturally-delivered male newborns (n = 5). Peripheral blood samples were obtained from healthy voluntary donors (n = 5). Karyotype analysis was carried out to determine the origin of the MSCs. Expression of TLR1-TLR10 was assessed by PCR. IL-1beta, IL-6 and IL-8 in MSCs were primed by Toll-like receptor agonists. MSCs primed by different Toll-like receptor (TLR) agonists and NK cells activated with IL-2 were co-cultured. CD107α were analyzed by flow cytometry. The NK cell-activating receptors NKp30, NKG2D, and DNAM-1 were analyzed by FCM. Also the levels of IL-10, IFN-γ and TNF-αwere evaluated by ELISA. Karyotype analyses indicated that cells were derived from the maternal matrix. The expression of TLR3 and 4 was higher when compared to the other molecules. In CD107α degranulation assay, MSCs primed by poly (I: C) and LPS reduced cytotoxic granules with significant differences (P < 0.05) when compared to the positive group. The NK cell-activating receptors NKp30, NKG2D, and DNAM-1 were high expression in activated NK group, but were inhibited during co-culture with MSC and primed MSC. The higher levels of IFN-γ, IL-10 and TNF-αwhich augment MSC suppressive function were secreted in B,C and D group. TLR3 and 4-primed MSCs reduced cytotoxicity of NK cells and protected from NK cell killing.
Mindfulness-Based Stress Reduction (MBSR) Effects on the Worries of Women with Poly Cystic Ovary Syndrome (PCOS)
Authors: Salajegheh Zahra , Ahmadi Atefeh , Shahrahmani Hadis , Jahani Yunes , Alidousti Katayoun , Amiri Fatemeh Nasiri , Salari Zohreh
Published: November 2022

Introduction

Polycystic ovary syndrome is one of the most common endocrine disorders in women that can cause anxiety, psychological distress and reduced quality of life. Therefore, the present study aimed to determine the effect of mindfulness-based stress reduction counseling on the worries of women with polycystic ovary syndrome.

Materials and methods

This quasi experimental was implemented on 60 women with polycystic ovary syndrome, referring to health centers in Kerman, Iran, from April to September 2021. In the intervention group, MBSR was conducted in eight 90-minute sessions twice a week. A researcher-made questionnaire with 34 questions (with six domains includes worries related to mental complications, interpersonal problems, non-pregnancy physical complications, pregnancy complications, sexual complications, and religion issues) on the worries of women with polycystic ovary syndrome were completed by the participants in two intervention and control groups as pre and posttest and one month later. 22 SPSS statistical software was used for analysis.

Results

The mean score of worries in the intervention group(48.18 ± 5.18) compared to the control group(75.73 ± 8.08) was significantly reduced in total and in all six domains immediately after the intervention (P < 0.0001). One month later also the total mean score of worries and subtitles decreased significantly(P < 0.0001), in intervention group (38.27 ± 3.58) in comparision with control group(76.13 ± 7.52).

Conclusion

Results showed that, the method of reducing stress based on mindfulness has caused a significant reduction in worries in the intervention group. Therefore, this method can be used to improve the mental health of this group of patients in health centers.