bluuebunny/crossref_metadata_2025 huggingface dataset:
CO2-Abscheidung und -Speicherung aus gesellschaftlicher Sicht
Authors: Pietzner Katja , Schumann Diana , Esken Andrea
Published: April 2018

Den zukünftigen Einsatz von CCS-Technologien knüpft die Bundesregierung in ihrem Energiekonzept nicht nur an technische Anforderungen. Auch organisierte Dialogverfahren mit unterschiedlichen Anspruchsgruppen sind geplant. Soziodemografische Faktoren können für die Gestaltung dieser Dialoge eine wichtige Rolle spielen.
Nachhaltigkeit mit Lebensfreude verbinden
Authors: Kleinert Uwe
Published: April 2018

Unternehmen müssen sich zunehmend den Erwartungen der Verbraucher im Hinblick auf nachhaltigen Konsum stellen. Sie können aber auch selbst einen Beitrag leisten, die Verbraucher für eine nachhaltigere Lebensweise zu gewinnen.
Cancún als Grundlage für eine Aufwärtsspirale im Klimaschutz?
Authors: Bals Christoph
Published: April 2018

Nicht mehr der große Wurf wird von Klimagipfeln der Vereinten Nationen (UN) erwartet, sondern das Zusammenspiel von drei Elemeten: der Verpflichtung auf das, wozu bereits Handlungsbereitschaft besteht, der Einigung auf Elemente, die Handlungsbereitschaft erhöhen, und eines Reviewprozesses, der neue Handlungsbereitschaft in verschärfte Ziele umsetzt.
Die unzulängliche Architektur des Clean Development Mechanism
Authors: Jürges Nataly
Published: April 2018

Emissionszertifikate machen Kohlenstoffdioxid-Einsparungen käuflich und sollen nachhaltige Entwicklung im globalen Süden fördern. Die Rechnung geht oft nicht auf. Es gibt in der Architektur des Clean Development Mechanism keine Anreize, Projekte mit einem Beitrag zur nachhaltigen Entwicklung umzusetzen. Stattdessen boomen Projekte an Industrieanlagen.
Fortschritte beim Wärmeschutz der Wohngebäude
Authors: Diefenbach Nikolaus , Cischinsky Holger , Rodenfels Markus , Clausnitzer Klaus-Dieter
Published: April 2018

Durch eine repräsentative Befragung von Hauseigentümern wurden Daten zum energetischen Zustand der deutschen Wohngebäude erhoben. Diese zeigen, dass höhere Modernisierungsraten beim Wärmeschutz notwendig sind, um die Klimaschutzziele zu erreichen.
Energieberatung in Haushalten auf Basis des Smartmetering
Authors: Raabe Thorsten , Sonnenschein Michael , Beenken Petra , Hüwel Andreas , Meinecke Christopher-Marcel
Published: April 2018

Stromeinsparungen sind ein zentraler Aspekt einer nachhaltigen Energienutzung. Ein softwarebasierter Beratungsansatz könnte helfen, denUmgang mit elektrischer Energie bewusster zu machen und so zu einem ange passten Stromkonsum beitragen.
Noch eine unbequeme Wahrheit
Authors: Oelinger Dietmar
Published: April 2018

Ein Kreuzfahrtschiff verursacht während der Fahrt so viele Schadstoffe wie 5.000.000 Autos. Während Autos, LKWs und Diesellokomotiven zunehmend mit Abgastechnik unterwegs sind, ist die Schifffahrt der letzte Verkehrsträger, bei dem Ökonomie und Ökologie weiterhin im Widerspruch stehen.
Transport temperatursensibler Waren unter Klimawandeleinfluss
Authors: Beermann Marina , Bramlage Henrik , Dolinski Marek
Published: April 2018

Der Klimawandel wirkt sich auch auf Prozesse und Strukturen der Logistikbranche aus.Hierbei sind die Unternehmen der Branche unterschiedlich betroffen. Welche Problemeund möglichen Anpassungsstrategien sind von Bedeutung?
Von der Kür zur Pflicht
Authors: Bergius Susanne
Published: April 2018

Nachhaltigkeitsberichterstattung wird mal als Bedingung effektiven Nachhaltigkeitsmanagements gesehen, mal als PR-Masche. In einer vierteiligen Serie beleuchtet ÖkologischesWirtschaften den Status quo sowie mögliche Zukunftsszenarien. In Teil 1 fordert die Autorin ver pflichtende Nachhaltigkeitsberichterstattung nach internationalen Standards.
Jetzt die richtigen Weichen stellen
Authors: Porsch Lucas
Published: April 2018

Wie bemisst man gesellschaftlichen Fortschritt? Im Projekt IN-STREAM haben Wissenschaftler(innen) entsprechende Indikatoren entwickelt, um in den drei Politikfeldern Nachhaltiges Wachstum, Ressourceneffizienz und Biodiversität die Politikentwicklung zu unterstützen.
Wachstumsneutrale Unternehmen
Authors: Dietsche Christian , Liesen Andrea
Published: April 2018

Einführung in das Schwerpunktthema
Gemeinsam sind wir groß
Authors: Gebauer Jana , Ziegler Rafael
Published: April 2018

Nur ein wachsendes Unternehmen ist ein gutes Unternehmen? Social Entrepreneurs sehen das anders und zeigen, was denkbar ist, wenn die eigene Größe keine Rolle spielt: Was erreichen wir mit anderen und für andere?
Unternehmen haben wenig Spielraum
Authors: Lange Steffen
Published: April 2018

Welche Rolle spielen Unternehmen bei der Frage, wie eine Wirtschaft ohne Wachstumstabil organisiert werden kann? Eine Antwort aus neoklassischer, keynesianischer und wettbewerbstheoretischer Sicht.
Suffizienz als Business Case
Authors: Palzkill Alexandra , Schneidewind Uwe
Published: April 2018

Über Suffizienz wird bisher ausschließlich als Orientierungsmuster für individuellen Konsum diskutiert. Der folgende Beitrag zeigt, dass die Debatte über Suffizienz durchaus Geschäftschancen für Unternehmen eröffnet.
Die Zukunft des europäischen Emissionshandels: Reform oder Abgesang?
Authors: Fuhr Lili
Published: April 2018

Die strukturellen Fehler und Schwächen des Europäischen Emissionshandels sind hinlänglich bekannt und wurden bereits im Detail analysiert. Aber hat eine darauf aufbauende Reform des Instruments überhaupt Aussicht auf Erfolg? Eine internationale Initiative stellt die Wirksamkeit des gesamten Systems infrage.
Green Business und Juniorenfirmen: Die nächste Generation der Ecopreneure?
Authors: Poser Nadine
Published: April 2018

Unternehmerisches Denken und Handeln unter Einhaltung ökologischer Prinzipien ist eine komplexe Aufgabe. Doch wie können Jugendliche an diese Thematik herangeführt werden? In einem Modellversuch arbeiteten Unternehmer mit Schulen zusammen, um so die Praxis des nachhaltigen Wirtschaftens zu erproben.
Welchen Beitrag können die Wirtschaftswissenschaften leisten?
Authors: Soete Birgit , Petschow Ulrich
Published: April 2018

Seit zwei Jahren ist die Transformationsforschung zur Nachhaltigkeit ein neues interdisziplinäres Forschungsfeld. Auch die Wirtschaftswissenschaften können mit ihren Methoden und Theorien dazu beitragen.
Naturkapital Deutschland
Authors: Hansjürgens Bernd , Wieler Barbara
Published: April 2018

Ökonomisch betrachtet stellt die Natur ein Kapital dar, aus dem Dividenden in Form von Ökosystemleistungen fließen. Doch obwohl Unternehmen mit Sach- und Humankapital sorgfältig umgehen, übersehen sie oft, dass auch das Naturkapital einen wichtigen Beitrag für die Wertschöpfung darstellt.
Umweltpolitik in Europa unter wachsenden Verteilungskonflikten
Authors: Zundel Stefan
Published: April 2018

Wer Umweltpolitik betreibt, muß heutzutage dicke Bretter bohren können. Der Antrieb durch die ökologische Bewegung ist eher bescheiden, wie der Deutsche Umwelttag noch einmal gezeigt hat. Deshalb geht die Umweltpolitik immer mehr über in ein zähes Bargaining um kleine und kleinste Geländegewinne. Zu den besonders dicken Brettern zählt die Umweltpolitik der Europäischen Ge-meinschaft: Nicht nur wegen der Schwierigkeiten, die eine ferne Bürokratie in Brüssel mit den Ent-wicklungen vor Ort hat und haben muß. Die Harmonisierung der nationalen Umweltpolitiken schafft zusätzliche neue Probleme.
Opinions of a group of Turkish patients regarding patients’ rights
Authors: Erer Türkan Meryem , Kadıoğlu Selim , Orekici Gülhan
Published: April 2018

The aim of this descriptive study is to determine a group of patients' awareness of patient rights, significance of patients’ rights for them and how they utilize these rights. The study was carried out with 100 of the patients who were admitted to the blood-taking unit of the outpatient services department of Mersin University Health Research and Application Center between September 1, - October 15, 2009, and agreed to participate in the study. The participants were asked to state whether they were knowledgeable about each of these rights, and whether they had ever benefited from them. Of the participants, 53% were knowledgeable about the 14 patients’ rights listed in the data collection form and 28% benefited from these rights.Although the study participants generally attached importance to patients' rights, they neither were knowledgeable about these rights nor benefited from them sufficiently.
Influence of the Air-Entraining Agent in the Concrete Coating on the Reinforcement Corrosion Process in Case of Simultaneous Action of Chlorides and Frost
Authors: Raczkiewicz W.
Published: April 2018

The paper presents the test results to evaluate the effect of air-entraining agent addition on the intensity of reinforcement corrosion in concrete with blast-furnace slag cement in the case of simultaneous action of chloride corrosion and frost. Two groups of reinforced concrete specimens were prepared for the study. The first group of specimens included air-entraining agent addition and the other group was prepared without air-entraining agent. The blast-furnace slag cement (CEM III/A) was used for the specimens. Two parallel reinforcing rods were placed in each specimen. The specimens were subjected to 120 cycles of freezing and thawing in 3% NaCl solution to induce corrosion on the reinforcement. To determine the occurrence of the reinforcement corrosion and estimate the corrosion activity the non-destructive electrochemical galvanostatic pulse method was used. On each specimen the corrosion current density of the reinforcement was measured as well as the reinforcement stationary potential and the concrete coating resistivity, i.e. values indicating the ongoing reinforcement corrosion. Measurements were made on all specimens in two steps: before freezing and thawing cycles in 3% NaCl solution and after the cycles. The analysis of the obtained results allowed to determine differences in corrosion processes intensity on the reinforced bars in the concrete specimens depending on whether or not the air-entraining agent was added. Based on the analysis it was found that in the case of simultaneous action of chloride corrosion and frost it is advisable to use both blast-furnace slag cement and air-entraining agent. The use of only blast-furnace slag cement (although it is a chloride resistant cement), without the addition of air-entraining agent is insufficient.
Supporting non-native speaker student writers making the transition from school to an English-medium university
Authors: Morrison Bruce , Evans Stephen
Published: April 2018

In an increasingly globalised world, English-medium tertiary education has become increasingly prominent and sought after. However, non-native speaker (NNS) school-leavers entering an English-medium university, whether as NNS local students or as international students, not only encounter those challenges faced by all freshmen students, but often also soon realise that there is a gap between the English language skills with which their school education had equipped them and those needed for effective English-medium tertiary study. Their lack of competence in academic writing presents perhaps the greatest challenge. This paper will examine data relating to NNS students’ experience as freshmen writers. These data are derived from a longitudinal study aiming to understand the language-related challenges faced by first and second year NNS students in an English-medium higher education institution in Hong Kong. The paper concludes by proposing two collaborative initiatives whereby English language teachers work with content subject teachers with the aim of providing language enhancement support that is relevant to the student’s programme of study and integrated into their content studies. With a global increase in the number of NNS students studying in English-medium universities (Murray 2016, Standards of English in higher education. Cambridge: Cambridge University Press), the challenges and possible ways of addressing these that are discussed in this paper are potentially directly relevant to universities in whichever country they operate.
The Role of Mineral Phases in the Biogas Production Technology
Authors: Geršl Milan , Kanduč Tjaša , Matýsek Dalibor , Šotnar Martin , Mareček Jan
Published: April 2018

In the field of electric power industry, renewable energy sources, fertilisers, reclamation, and waste management, biomass is widely studied and used. Minerals are present in every step of biogas transformation, but their forms, occurrence, and composition have not been studied yet. However, there is no comprehensive study research that would address the presence of mineral phases in the process of biogas production. This aim of the study is determination of the amount and composition of the mineral phases present in fermentation residues resulting from different production technologies. Digestate mineral composition was analysed using 46 samples from agricultural biogas plants and university testing biogas reactor. The majority of samples contained the amorphous phase. Minority phases consisted of quartz, albite, orthoclase, muscovite, and amphibole. Opal-CT was found in eleven samples (1.26 to 12.1% wt.). The elements present in gas-liquid fluids or in liquids, gases and aerosols within the biogas technology system may create mineral phases, namely the amorphous phase or the crystalline phase under certain conditions. Opal-CT may enter the fermenter as part of plant tissues referred to as phytoliths, or as an unwanted admixture of different origin. It may also originate from the present amorphous SiO2.
New Methods of Natural Gas Adjusting for Technological Purposes Based on Modern Filtration Materials
Authors: Jackiewicz-Zagórska Anna , Szwast Maciej , Gac Jakub , Werner Łukasz , Zalewski Michał , Jakubiak Szymon
Published: April 2018

Reservoir gas treatment technologies currently in use require significant investments in equipment and materials. Considering the foregoing, new technologies are sought for treatment and separation of components of extracted gas. They will guarantee compliance with gas quality requirements arising from applicable standards while at the same time lowering the costs of investment, service and environmental protection. Therefore, the purpose of this work was to develop an efficient mobile technology for natural gas treatment, on the basis of advanced filtering and coalescing materials and membranes, which eliminate the existing, economically non-viable processes and reduce the costs related to development of new reservoirs. The purpose of designed materials was removal of solid particles, water, higher hydrocarbons and nitrogen from natural gas. Presented integrated filtration and membrane system, characterized by a highly compact design enabling installation in container stations and transporting the system between specific points of production.
Computer Simulation of Chemical Coagulation and Sedimentation of Suspended Solids
Authors: Wardzyńska Regina , Smoczyński Lech , Załęska-Chróst Beata
Published: April 2018

The computer program ZB2 was used to study simulated coagulation rate for the system containing spherical sol particles and spherical coagulant particles. The system performance was verified to the particle-cluster model of a fast and perikinetic coagulation process that fulfils Smoluchowski and/or Muller equations. The rate of the coagulation process satisfied both the kinetic equation of a first-order reaction and a second-order reaction. However, chosen concepts and models in the theory of bidispersive sol coagulation have been negatively verified. Also, attempts have been made to modify the Muller integral equation for selected boundary conditions.
Validation of Microsatellite Markers of Pl Resistance Genes to Downy Mildew of Sunflower
Authors: Solodenko A.
Published: April 2018

Simple sequence repeats (SSR) polymorphism of 34 microsatellite loci (LG1, 8 and 13) was studied in lines carrying the downy mildew resistance genes Pl and lines with no Pl. The microsatellite loci ORS328 and ORS781 were selected as markers for genes Pl6 and Pl8 in lines HA 335 and QHP-1, respectively. Markers were identified for gene PlARG in RHA 419 and some accessions of H. argophyllus. The SSR markers ORS509, ORS605, ORS610, ORS1182 and ORS1039 were proven to reliably identify the parental line carrying PlARG gene, control and select the heterozygous F1 hybrids and identify homozygous genotypes in F2 generations. Obtained results indicate the necessity of validation of the markers in various germplasm pools and breeding collections. The SSR markers that are tightly linked to Pl6, Pl8, PlARG would be useful in the sunflower breeding. PlARG homozygous F2 segregants, developed and identified with marker assisted selection in this study, are recommended for further breeding as a new source of genetically determined resistance to downy mildew.
A matrix-resistant HPTLC method to quantify monosaccharides in wood-based lignocellulose biorefinery streams
Authors: Oberlerchner Josua Timotheus , Böhmdorfer Stefan , Rosenau Thomas , Potthast Antje
Published: April 2018

A new and robust high performance thin-layer chromatography (HPTLC) method has been developed for carbohydrate analysis in biorefinery streams. The samples can be applied directly from industrial streams without any purification or pretreatment, saving time and resources and avoiding potential alterations to the samples’ composition. The main neutral monosaccharides found in wood-based lignocellulosic biomass – glucose, xylose, mannose, arabinose and galactose – can be separated and quantified using acetonitrile:1-pentanol:water (4:1:1, v/v/v) on silica impregnated with a phosphate buffer. Common minor compounds – glucuronic acid, galacturonic acid, rhamnose, cellobiose and hydroxymethylfurfural (HMF) – can also be detected qualitatively. Matrix compounds, which are a major part of biomass, do not interfere with the analysis. Validation showed that the method is selective, has good repeatability and has limits of detection and quantification in the nanogram range. The visual fingerprint offers information on the samples’ constituents in the case of unknown samples. Also, incomplete hydrolysis is indicated by the visibility of oligosaccharides.
The Creative Industry and South African Intellectual Property Law
Authors: Ncube Caroline B.
Published: April 2018

This paper seeks to provide a more nuanced view of the creative industry that goes beyond assertions of its contribution to economic growth, which, it is then further argued, requires stringent copyright protection to ensure development. It argues that a critical first step is to optimize an existing copyright framework by addressing its inherent entrepreneurial challenges to better enable authors to garner economic returns. These challenges are identified before the paper delineates the creative industries in South Africa and related policies. It then turns to the ongoing copyright policy formulation process before setting out current and proposed copyright legislative provisions. The paper contends that essential aspects regarding both the creative and commercial aspects must be tackled first. At the creative stage, authors’ inability to use a large range of source works because of the fear of copyright infringement claims can be addressed by elaborating exceptions and limitations. On the commercial front, entrepreneurial capacity building for authors and curbing unfair author, publisher and intermediary contracts is vital. The use of statutory devices such as the reversionary interest, to recover lost or diminished opportunities to obtain direct financial gain from copyright work, could also be considered. Enhancing the viability of collecting societies and ensuring that royalties are paid to authors would also be a critical intervention.
Language as an Instrument for Dispute Resolution in Modern Justice
Authors: Drabarz Anna K. , Kałużny Tomasz , Terrett Stephen
Published: April 2018

The frustration in Polish society arising from excessive costs of conducting court proceedings and lengthy delays for dispute resolution has resulted in a genuine limitation in access to judicial justice for citizens. This paper argues that the answer to the dilemma between ensuring both justice and efficiency lies in language being a tool for the active participation of the parties in building mutual trust and shaping solutions in conflictual circumstances. How should the postulate of effective communication leading to dispute resolution in modern justice be achieved? The authors present the advantages of oral communication in proceedings on the way to finding agreement, pointing out the content and quality of language that make dispute resolution possible.
Polish “Entrepreneur” and EU “Undertaking”: Multilingualism and Differences in Legal Identification
Authors: Etel Maciej
Published: April 2018

The European Union and its member-states’ involvement in the economic sphere, manifesting itself in establishing the rules of entrepreneurs’ functioning – their responsibilities and entitlements – requires a precise determination of the addressees of these standards. Proper identification of an entrepreneur is a condition of proper legislation, interpretation, application, control and execution of the law. In this context it is surprising that understanding the term entrepreneur in Polish law and in EU law is not the same, and divergences and differences in identification are fundamental. This fact formed the objective of this article. It is aimed at pointing at key differences in the identification of an entrepreneur between Polish and EU law, explaining the reasons for different concepts, and also the answer to the question: May Poland, as an EU member-state, identify the entrepreneur in a different way than the EU?
A Logical Conceptualization of Knowledge on the Notion of Language Communication
Authors: Wybraniec-Skardowska Urszula
Published: April 2018

The main objective of the paper is to provide a conceptual apparatus of a general logical theory of language communication. The aim of the paper is to outline a formal-logical theory of language in which the concepts of the phenomenon of language communication and language communication in general are defined and some conditions for their adequacy are formulated. The theory explicates the key notions of contemporary syntax, semantics, and pragmatics. The theory is formalized on two levels: token-level and type-level. As such, it takes into account the dual – token and type – ontological character of linguistic entities. The basic notions of the theory: language communication, meaning and interpretation are introduced on the second, type-level of formalization, and their required prior formalization of some of the notions introduced on the first, token-level; among others, the notion of an act of communication. Owing to the theory, it is possible to address the problems of adequacy of both empirical acts of communication and of language communication in general. All the conditions of adequacy of communication discussed in the presented paper, are valid for one-way communication (sender-recipient); nevertheless, they can also apply to the reverse direction of language communication (recipient-sender). Therefore, they concern the problem of two-way understanding in language communication.
Examining the Effects of an Otago-Based Home Exercise Program on Falls and Fall Risks in an Assisted Living Facility
Authors: Beato Morris , Dawson Nicole , Svien Lana , Wharton Tracy
Published: April 2018

Background and Purpose:

The Otago exercise program is a strengthening, balance, and walking program designed to decrease falls among community-dwelling older adults. Few studies have examined the effects of the Otago program in an assisted living environment. The purpose of the current study was to assess the effects of an Otago-based home exercise program in decreasing falls and the risk of falls among older adults living in an assisted living facility.

Methods:

A retrospective chart review of 30 individuals residing at either of 2 assisted living facilities in central Florida was undertaken. Participants had a mean age of 87 years, were at risk for falls as determined by the Tinetti Performance-Oriented Mobility Assessment (POMA), and were provided with an Otago-based intervention by home health physical therapy. The outcome measures were the number of falls in the previous year, the number of falls in the year following the intervention, and Tinetti POMA scores pre- and post-intervention.

Results and Discussion:

The mean (SD) number of falls significantly decreased from 1.4 (0.9) to 0.5 (0.7) fall per person per year after home health physical therapy with the tailored Otago based-exercise intervention. The intervention resulted in a statistically significant improvement in Tinetti POMA scores from 11.8 (2.5) to 17.6 (3.8).

Conclusions:

An Otago-based strengthening, balance, and walking home exercise program can potentially be used to decrease the number of falls and the risk of falling among older adults residing in an assisted living facility.
Canonical TGF-β Signaling Negatively Regulates Neuronal Morphogenesis through TGIF/Smad Complex-Mediated CRMP2 Suppression
Authors: Nakashima Hideyuki , Tsujimura Keita , Irie Koichiro , Ishizu Masataka , Pan Miao , Kameda Tomonori , Nakashima Kinichi
Published: April 2018

Functional neuronal connectivity requires proper neuronal morphogenesis and its dysregulation causes neurodevelopmental diseases. Transforming growth factor-β (TGF-β) family cytokines play pivotal roles in development, but little is known about their contribution to morphological development of neurons. Here we show that the Smad-dependent canonical signaling of TGF-β family cytokines negatively regulates neuronal morphogenesis during brain development. Mechanistically, activated Smads form a complex with transcriptional repressor TG-interacting factor (TGIF), and downregulate the expression of a neuronal polarity regulator, collapsin response mediator protein 2. We also demonstrate that TGF-β family signaling inhibits neurite elongation of human induced pluripotent stem cell-derived neurons. Furthermore, the expression of TGF-β receptor 1, Smad4, or TGIF, which have mutations found in patients with neurodevelopmental disorders, disrupted neuronal morphogenesis in both mouse (male and female) and human (female) neurons. Together, these findings suggest that the regulation of neuronal morphogenesis by an evolutionarily conserved function of TGF-β signaling is involved in the pathogenesis of neurodevelopmental diseases.

SIGNIFICANCE STATEMENTCanonical transforming growth factor-β (TGF-β) signaling plays a crucial role in multiple organ development, including brain, and mutations in components of the signaling pathway associated with several human developmental disorders. In this study, we found that Smads/TG-interacting factor-dependent canonical TGF-β signaling regulates neuronal morphogenesis through the suppression of collapsin response mediator protein-2 (CRMP2) expression during brain development, and that function of this signaling is evolutionarily conserved in the mammalian brain. Mutations in canonical TGF-β signaling factors identified in patients with neurodevelopmental disorders disrupt the morphological development of neurons. Thus, our results suggest that proper control of TGF-β/Smads/CRMP2 signaling pathways is critical for the precise execution of neuronal morphogenesis, whose impairment eventually results in neurodevelopmental disorders.
GREB1 isoforms regulate proliferation independent of ERα co-regulator activities in breast cancer
Authors: Haines Corinne N , Braunreiter Kara M , Mo Xiaokui Molly , Burd Craig J
Published: April 2018

Activation of the transcription factor estrogen receptor α (ERα) and the subsequent regulation of estrogen-responsive genes play a crucial role in the development and progression of the majority of breast cancers. One gene target of ERα, growth regulation by estrogen in breast cancer 1 (GREB1), is associated with proliferation and regulation of ERα activity in estrogen-responsive breast cancer cells. The GREB1 gene encodes three distinct isoforms: GREB1a, GREB1b and GREB1c, whose molecular functions are largely unknown. Here, we investigate the role of these isoforms in regulation of ERα activity and proliferation. Interaction between GREB1 and ERα was mapped to the amino terminus shared by all GREB1 variants. Analysis of isoform-specific regulation of ERα activity suggests none of the GREB1 isoforms possess potent co-regulator activity. Exogenous expression of GREB1a resulted in elevated expression of some ER-target genes, independent of ERα activity. Despite this slight specificity of GREB1a for gene regulation, exogenous expression of either GREB1a or GREB1b resulted in decreased proliferation in both ER-positive and ER-negative breast carcinoma cell lines, demonstrating an ER-independent function of GREB1. Interestingly, we show an increase in the expression of GREB1b and GREB1c mRNA in malignant breast tissue compared to normal patient samples, suggesting a selective preference for these isoforms during malignant transformation. Together, these data suggest GREB1a has an isoform-specific function as a transcriptional regulator while all isoforms share an ER-independent activity that regulates proliferation.
Multiple Arginine Residues Are Methylated in Drosophila Mre11 and Required for Survival Following Ionizing Radiation
Authors: Yuan Qing , Tian Ran , Zhao Haiying , Li Lijuan , Bi Xiaolin
Published: April 2018

Mre11 is a key player for DNA double strand break repair. Previous studies have shown that mammalian Mre11 is methylated at multiple arginines in its C-terminal Glycine-Arginine-Rich motif (GAR) by protein arginine methyltransferase PRMT1. Here, we found that the Drosophila Mre11 is methylated at arginines 559, 563, 565, and 569 in the GAR motif by DART1, the Drosophila homolog of PRMT1. Mre11 interacts with DART1 in S2 cells, and this interaction does not require the GAR motif. Arginines methylated Mre11 localizes exclusively in the nucleus as soluble nuclear protein or chromatin-binding protein. To study the in vivo functions of methylation, we generated the single Arg-Ala and all Arginines mutated flies. We found these mutants were sensitive to ionizing radiation. Furthermore, Arg-Ala mutated flies had no irradiation induced G2/M checkpoint defect in wing disc and eye disc. Thus, we provided evidence that arginines in Drosophila Mre11 are methylated by DART1 methytransferase and flies loss of arginine methylation are sensitive to irradiation.
Un análisis de los factores relacionados con la motivación de los estudiantes de italiano de la Universidad Kyoto Sangyo (Japón)
Authors: Diodato Francesco
Published: April 2018

Este trabajo está basado en la recolección de datos realizada a través de dos cuestionarios enviados a los estudiantes de primer y segundo año de italiano de la Universidad Kyoto Sangyo (Japón) en mayo y julio de 2015. Los cuestionarios indagaron las opiniones de los estudiantes sobre los factores que, en su opinión, motivaron su estudio del italiano. Dichos cuestionarios se aplicaron en dos momentos diferentes del curso. Los resultados mostraron consideraciones relacionadas con una amplia variedad de ámbitos, dentro de los cuales están los factores sociales y del sistema de valores japoneses, así como la organización de la universidad, su ambiente e incluso el papel de la familia. El análisis de las opiniones demostró cómo estos elementos también han influido en la formación de sus convicciones hacia el estudio. Sin embargo, los estudiantes indicaron que su motivación también está estrechamente relacionada con la estructura y el desarrollo de las clases; de hecho, perciben como motivadoras las actividades cuyas características están vinculadas con un aprendizaje activo. Esto confirma la conexión que hay entre dicha estrategia de aprendizaje y la motivación y, por consiguiente, la memoria. Surgieron además otros factores que complementan la forma en la que se llevan a cabo las actividades: un ambiente libre de tensión en el aula, así como un profesor divertido, entusiasta y alegre que tenga una actitud amable y sonriente. También surgieron opiniones con respecto a temas controvertidos pero siempre relevantes, tales como el papel de la evaluación, la tarea y el horario de clase.
Apresentação - Volume 3 – Número 1
Authors: Filho Reginaldo Pujol
Published: April 2018

Apresentação - Volume 3 – Número 1
Les Gommes, de Alain Robbe-Grillet: vozes de coexistência
Authors: Müller Iuri Almeida
Published: April 2018

Espécie de marco inaugural do chamado novo romance francês, Les Gommes, de Alain Robbe-Grillet, também antecipa as questões que interessariam o autor durante toda a sua obra: o problema da linearidade no romance, a presença de distintos planos na narrativa, a descrição e a visão como maneira de enxergar e narrar um mundo recém-tocado pela perplexidade. Há, nas vozes de Les Gommes, um novo tratamento do tempo na obra literária e a constituição de um romance que, a todo momento, se abre a diversas possibilidades de se contar uma história. Aqui, Les Gommes é lido como romance policial ao revés, exemplar que, simultaneamente, nega e dignifica o gênero.
A voz do autor: a metaficção na criação de personagens em “Diário para um conto”, de Cortázar
Authors: Urbim Luciana Pastorini
Published: April 2018

Este artigo apresenta uma análise da narrativa breve “Diário para um conto”, de Júlio Cortázar, retirado do livro Fora de Hora (1982), acerca dos mecanismos utilizados pelo escritor na criação de um conto, em especial de uma personagem, Anabel, em torno da qual orbita a história a ser contada. Com a finalidade de expor a problemática vivenciada pelo escritor na tarefa de escritura e de dar vida a um ser ficcional, Cortázar se utiliza de recursos metaficcionais que possibilitam espelhar a experiência criativa de um escritor ao tentar construir uma narrativa e seus elementos, evidenciando, sobretudo os conflitos em que ele se vê diante da criação da personagem, bem como seus limites entre realidade e ficção.
Enciclopédia polifônica: a construção dos textos e das vozes em Art Book
Authors: Moreschi Bruno
Published: April 2018

O artigo discute o conteúdo textual e as vozes presentes em Art Book, uma enciclopédia de artistas e obras criada a partir da identificação dos clichês encontrados em outras publicações do gênero e tema do mestrado em Artes Visuais na Unicamp do pesquisador e artista Bruno Moreschi. As vozes narrativas presentes em Art Book são diversas, e o texto aqui discute algumas de suas características e como elas atuam conjuntamente na criação de um efeito de pluralidade e veracidade presente no livro, que pode ou não se percebido como ficcional pelo leitor.
Fragmentos de um discurso criativo: notas para uma metodologia de composição da história
Authors: Cruz Marcelo Maldonado
Published: April 2018

Resultado de uma antiga necessidade de efabulação, o processo de contar uma história requer do criador uma capacidade específica de reunir elementos da vivência humana e dar-lhes um significado maior através da dramatização. No entanto, uma história não surge na mente de um autor em sua forma acabada, mas, sim, aos pedaços, em fragmentos. Tal como o enamorado, no discurso amoroso de Roland Barthes, às voltas com o objeto de sua paixão, o autor promove uma intensa investigação acerca dos elementos constitutivos da história que quer contar, valendo-se muitas vezes de uma estrutura mítica para coordená-los. Este ensaio reúne algumas anotações para uma metodologia do processo criativo, desde a concepção da ideia original até a fase de estruturação da história, através da análise de alguns princípios básicos e conceitos norteadores como “lei do conflito”, “princípio da progressão”, “forças do antagonismo”, “jornada do herói”, entre outros.
Éramos seis
Authors: Eger Talita Jabs
Published: April 2018

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Two new species of plant parasitic nematodes Hirschmanniella bananae n.sp. (Nematoda: Pratylenchidae) and Scutellonema tanlamense n.sp. (Nematoda: Hoplolaimidae) associated with banana in Vienam
Authors: Chau Nguyen Ngoc
Published: April 2018

During investigation on the plant parasitic nematodes on bananas in North and Central provinces of Vietnam carried out in 1995 two new species were found, Hirschmanniella bananae n.sp. and Scutellonema tanlamese n.sp. Hirschmanniella bananae n.sp. is characterized by having 6 annuli in lip region, areolation of entire lateral field and presence of a mid-terminal mucro at the tail end. The new species is close to H. shamini Ahmad, 1972 but differs from H. shamini by longer body length in females (1450-1750 µm vs 1190-1360 mm), longer stylet longer (18-20.5 mm vs 16-19 mm) and longer spicule longer (28-29.5 mm vs 22-25 mm). Scutellonema tanlamese n.sp. is characterized by lip region with three annuli and numerous longitudinal ridges (18) in basal annulus and structure of lateral field at the tail region with inner lines extending to tail terminus. The new species is closest to S. brachyurus type B (African populations) described by Van Den Berg et al. in 2013 but can be distinguished by body length shorter (643-708 µm vs 777-820 µm), stylet length shorter ((24.5-26.5 µm vs 28-29.5 mm, and also by structure of lateral field with two inner lines going throught at tail region and vulval edge distinct with epiptygma appearing double and folded into vagina.   Citation: Nguyen Ngoc Chau, 2017. Two new species of plant parasitic nematodes Hirschmanniella bananae n.sp. (Nematoda: Pratylenchidae) and Scutellonema tanlamense n.sp. (Nematoda: Hoplolaimidae) associated with banana in Vienam. Tap chi Sinh hoc, 39(3): 253-263. DOI: 10.15625/0866-7160/v39n3.10665. *Corresponding author: nguyengochau.iebr@gmail.com Received 19 August 2017, accepted 12 September 2017
Molecular phylogenetic identification of the giant freshwater softshell turtle in the Hoan Kiem lake, Hanoi, Vietnam
Authors: Viet Thanh Tran Thi , Long Phan Ke , Minh Nguyen Trung , Tam Nguyen Minh
Published: April 2018

To clarify the taxonomic status of the giant freshwater softshell turtle in the Hoan Kiem Lake (2016), we analyzed four genes COI, COII, Cytb and ND4. Genomic DNA was extracted from the nail tissue. The nucleotide sequences of the four genes were determined with 652bp, 621bp, 993bp and 731bp, respectively and used to analysis of genetic relationships, together with similar sequences published on GenBank belonging to Rafetus swinhoei, Rafetus eupharaticus and Polychelys cantorii. The analysis indicated very low levels of the K2P divergence were found in all the four genes between the turtle species at Hoan Kiem Lake and R. swinhoei (0-3.7%), while very high levels of divergence were observed between the turtle species at Hoan Kiem Lake and R. eupharaticus (8.6-12.8%), and P. cantorii (14.9-25.4%). Phylogenetic analysis showed that the turtle species at Hoan Kiem Lake together with R. swinhoei to form a cluster with strong bootstrap value 100%. The results showed that the turtle species at Hoan Kiem Lake was R. swinhoei.
A proteomic analyses to assess the effects of chronic exposure of bisphenol a to adult zebrafish (Danio rerio)
Authors: Mai Ngo Thi , Lan Chi Do Hong , Nga Le Phi
Published: April 2018

Bisphenol A (BPA) is an endocrine-disrupting compound found in the environment as well as in the plastic food containers for human use. Most impact of this compound to animals is acute toxicity to the liver of young individuals, but little is known about chronic toxicity to the adult liver. Here we report that, when adult zebrafish were exposed to various concentration of bisphenol A for 60 days, we have the number of liver protein spots in 2D-PAGE gels was reduced to 15-25%. Protein identification by HPLC-ESI (electron spray ionization) revealed that sixteen 2D-PAGE spots were remarkably altered and majority of them are related to metabolic energy system; e.g., liver basic L-lactate dehydrogenase B-B chain, ATP synthase subunit beta, and intracellular fatty acid-binding proteins. Besides, heat shock protein 60kDa 1 (HSPD1), which is known to  associate with inflammation, was over-expressed in fish liver after exposure to 100 µg/l BPA, but not at lower concentrations of BPA. Thus, chronic exposure to environmental BPA may cause liver dysfunctions. Sixteen hepatic proteins identified in this study would be potential biomarkers for chronic BPA toxicity.   Citation: Ngo Thi Mai, Do Hong Lan Chi, Le Phi Nga, 2017. A proteomic analyses to assess the effects of chronic exposure of bisphenol a to adult zebrafish (Danio rerio). Tap chi Sinh hoc, 39(3): 333-341. DOI: 10.15625/0866-7160/v39n3.98995. *Corresponding author: lephinga1@hotmail.com, lephinga@hcmut.edu.vn Received 12 December 2016, accepted 20 August 2017
Optimization of fermentation conditions for squalene production by heterotrophic marine microalgae Schizochytrium mangrovei PQ6
Authors: Ha Nguyen Cam , Minh Hien Hoang Thi , Thom Le Thi , Huong Quynh Hoang Thi , Hong Dang Diem
Published: April 2018

Squalene is currently known as chemopreventive agents in reducing the incident of coronary heart disease and cancer. It is also a strong antioxidant used extensively in the food and cosmetic industries. Microbial sources of squalene are being explored in recent years. The objective of this study was to investigate fermentation conditions of Schizochytrium mangrovei PQ6 for high squalene production in 30L bioreactor. Squalene production was influenced by various nutritional factors such as glucose, nitrogen, vitamins etc. and physical factors such as batch and fed-batch fermentation. Our study established that fed-batch fermentation with nitrogen source of 1.2% yeast extract and 6.4% monosodium glutamate and 0.4% mixture of vitamin B1, 6 and 12 as optimal culture condition for maximal production of squalene by S. mangrovei PQ6. In fed-batch culture, the glucose concentration feeding was added up to 22% into the culture at 36 h of fermentation. The maximum squalene production was achieved 6.9 ± 0.1 g/L at 48 h of fed-batch fermentation, respectively. The values were 2-3 times more than that in fed-batch cultivation without adding mixture of vitamins and 4-6 times more than that in batch cultivation. Our obtained results are a breakthrough for the development of alternative squalene source from heterotrophic marine microalgae of Schizochytrium mangrovei PQ6 species for health-promoting functional foods.   Citation: Nguyen Cam Ha, Hoang Thi Minh Hien, Le Thi Thom, Hoang Thi Huong Quynh, Dang Diem Hong, 2017. Optimization of fermentation conditions for squalene production by heterotrophic marine microalgae Schizochytrium mangrovei PQ6. Tap chi Sinh hoc, 39(3): 349-358. DOI: 10.15625/0866-7160/v39n3.9130. *Corresponding author: hoangminhhien@yahoo.com; ddhong60vn@yahoo.com Received 6 January 2017, accepted 20 August 2017
Fractionation of phenolic compounds from Sonneratia apetala pneumatophores and their bioactivities
Authors: Mai Nguyen Sao , Tan Dao Van
Published: April 2018

Sonneratia apetala pneumatophore samples were collected from Xuan Thuy National Park, and then 80% methanol extract was fractionated through Sephadex LH-20 column chromatography. After elution with 95% ethanol and 50% acetone, two phenolic fractions were obtained from the crude extract of of S. apetala pneumatophore. The acetone fraction consisted of tannins having high antioxidant activity, 1.14 times higher than that of ascorbic acid. This fraction also have higher activity of α-amylase inhibition (IC50 0.83 mg/mL) and R. sativus root growth inhibition (IC50 6.4 mg/mL) than did the ethanol fraction. In contrast, ethanol fraction consisted of low-molecular phenolic compounds having inhibitory effects on Samonella tiphimurium growth. Such antibacterial activity was not observed in the acetone extract. Citation: Nguyen Sao Mai, Dao Van Tan, 2017. Fractionation of phenolic compounds from Sonneratia apetala pneumatophores and their bioactivities. Tap chi Sinh hoc, 39(4): 451-456. DOI: 10.15625/0866-7160/v39n4.10708.*Corresponding author: daotanvn@yahoo.comReceived 19 September 2017, accepted 12 December 2017
Biodiversity of Tintinnids (Tintinnida) in Khanh Hoa - Binh Thuan waters
Authors: Kieu Nguyen Thi , Luom Phan Tan , Vinh Nguyen Tam , Lam Nguyen Ngoc , P. Montoya Joseph , Hai Doan Nhu
Published: April 2018

Tintinnidsarean important protozoan group in the aquatic food web and had been widely studied in various waters. There are about 1000 known species in the world. However, there have beenvery few taxonomic studies in Vietnam and therefore the number of Tintinnid taxa and their distribution are poorly known. Thepresent study documents 65 tintinnids species belonging to 30 genera and 13 families in samples collected from Khanh Hoa - Binh Thuan waters in 2016 and 2017. There were 17 new taxa records for Vietnam protozoan fauna, raising the number of tintinnids recorded in Vietnam to 125 taxa. Tintinnid assemblages in Khanh Hoa-Binh Thuan waters shared about 17 species with Ha Long Bay, 32 species with Con Co island and 26 species with coastal waters of South Vietnam. Analysis of species diversity shows that the Shannon diversity index H' varied from 1.5 to 2.6. Distribution of species numbers and diversity in the Khanh Hoa - Binh Thuan waters revealed possible combined effects of hydrographical activities (e.g. upwelling), Mekong river influent (e.g. salinity), and food available on tintinnid communities.   Citation: Nguyen Thi Kieu, Phan Tan Luom, Nguyen Tam Vinh, Nguyen Ngoc Lam, Josepth P. Montoya, Doan Nhu Hai, 2017. Biodiversity of Tintinnids (Tintinnida) in Khanh Hoa - Binh Thuan waters. Tap chi Sinh hoc, 39(4): 421-433. DOI: 10.15625/0866-7160/v39n4.11033. *Corresponding author:haidoan-ion@planktonviet.org.vn Received 19 September 2017, accepted 12 December 2017
Development of cookies from agroindustrial by-products
Authors: Deamici Kricelle Mosquera , Oliveira Lucas Carvalho de , Rosa Gabriela Silveira da , Zavareze Elessandra da Rosa , Oliveira Elizangela Gonçalves de
Published: April 2018

Abstract The aim of this study was to evaluate the potential use of dry grape marc obtained of drying and milling operations with parboiled rice bran for cookies production. By-products were characterized with respect to moisture content, proteins, lipids, carbohydrates, fiber, and ashes, besides the analysis of absorption index, solubility in water and acidity. The proposed formulations were prepared by varying the amount of by-products to replace wheat flour. Elaborate cookies were subjected to microbiological and sensory analysis. The formulation with greater acceptance contained 5% of flour grape marc and 10% of parboiled rice bran. The physicochemical characterization of the cookies with greater acceptance was highlighted by the protein and fiber that presented 5.15% and 28.42%, respectively. The result obtained from this study shows a suitable product for consumption for microbiological as well as nutritional point of view.
Fluxos de CO2 em Plantio de Palma de Óleo no Leste da Amazônia
Authors: Fonseca Luiz Carlos Neves da , Araújo Alessandro Carioca de , Manzi Antônio Ocimar , Carvalho Claudio José Reis de , Oliveira Leonardo Ramos de
Published: April 2018

Resumo Os ecossistemas terrestres são importantes para compreender as trocas de CO2 entre superfície e atmosfera. Este trabalho apresenta as condições atmosféricas e medidas de CO2 em palma de óleo com híbrido interespecífico no leste da Amazônia, durante ano de El niño. O experimento foi realizado em Moju - Pará, onde foi instalada uma torre micrometeorológica para obtenção de dados meteorológicos e de CO2 durante o período de janeiro-dezembro de 2015. Os valores médios diurnos de absorção de CO2 atingiram o máximo por volta do meio dia, com 22,3 (± 0,98) μmol m-2 s-1 no período chuvoso e 21,0 (± 0,47) μmol m-2 s-1 no período menos chuvoso. Houve pouca variação noturna nos valores médios de emissão de CO2, 5 (± 0,20) μmol m-2 s-1, em ambos os períodos. Os valores médios diurnos da concentração de CO2 foram menores e os valores médios noturnos foram maiores. Em geral, o ciclo diário de troca de CO2 apresentou diferença entre o período chuvoso e menos chuvoso. Esses resultados contribuem para melhor compreensão da tendência temporal dos fluxos de CO2 em cultivo agrícola em ano de seca no leste da Amazônia, gerando informações novas sobre a interação entre palma de óleo e atmosfera.