bluuebunny/crossref_metadata_2025 huggingface dataset:
Assessment of Dysfunction in the Urinary System as Well as Comfort in the Life of Women during and after Combination Therapy Due to Ovarian and Endometrial Cancer Based on the SWL, II-Q7 and UDI-6 Scales
Authors: Opławski Marcin , Smoczyńska Magdalena , Grabarek Beniamin Oskar , Boroń Dariusz
Published: March 2021

This work aimed to assess the influence of oncological combination therapy that was done on endometrial or ovarian cancer and how the urinary system is influenced as well as the quality of life in comparison to a group of female patients after the removal of the uterus with appendages due to endometrial cancer, which did not require the supplementation of therapy after operative treatment. The study included 87 patients with endometrial cancer, where, after the removal of the uterus, there was no need for conducting adjuvant therapy (C), as well as 92 female patients with endometrial cancer or 38 patients with ovarian cancer in whom combination therapy was conducted (group A, B). The assessment of the quality of life was conducted using the questionnaires: Satisfaction Life Scale (SWLS), Incontinence Impact Questionnaire, Short Form (IIQ-7), and Urogenital Distress Inventory (UDI-6) for three, six, nine, and 12 months after the conclusion of oncological treatment. It was observed that there was a statistically significant decrease in the quality of life in female patients who underwent combination therapy in comparison to a group in whose treatment only included surgery (p < 0.05). The risk of developing urinary incontinence increases alongside an increase in the scope of the operation and in the case of supplementing treatment with brachytherapy in comparison to chemotherapy.
Effectiveness and Quality of Implementing a Best Practice Model of Care for Low Back Pain (BetterBack) Compared with Routine Care in Physiotherapy: A Hybrid Type 2 Trial
Authors: Schröder Karin , Öberg Birgitta , Enthoven Paul , Hedevik Henrik , Fors Maria , Abbott Allan
Published: March 2021

Low back pain (LBP) occurs in all ages and first-line treatment by physiotherapists is common. The main aim of the current study was to evaluate the effectiveness of implementing a best practice model of care for LBP (intervention group—BetterBack☺ MoC) compared to routine physiotherapy care (control group) regarding longitudinal patient reported outcomes. The BetterBack☺ MoC contains clinical guideline recommendations and support tools to facilitate clinician adherence to guidelines. A secondary exploratory aim was to compare patient outcomes based on the fidelity of fulfilling a clinical practice quality index regarding physiotherapist care. A stepped cluster randomized design nested patients with LBP in the three clusters which were allocated to control (n = 203) or intervention (n = 264). Patient reported measures were collected at baseline, 3, 6 and 12 months and analyzed with mixed model regression. The primary outcome was between-group changes from baseline to 3 months for pain intensity and disability. Implementation of the BetterBack☺ MoC did not show any between-group differences in the primary outcomes compared with routine care. However, the intervention group showed significantly higher satisfaction at 3 months and clinically meaningful greater improvement in LBP illness perception at 3 months and quality of life at 3 and 6 months but not in patient enablement and global impression of change compared with the control group. Physiotherapists’ care that adhered to all clinical practice quality indices resulted in an improvement of most patient reported outcomes with a clinically meaningful greater improved LBP illness perception at 3 months and quality of life at 3 and 6 months, significantly greater improvement in LBP illness perception, pain and satisfaction at 3 and 6 months and significantly better enablement at all time points as well as better global improvement outcomes at 3 months compared with non-adherent care. This highlights the importance of clinical guideline based primary care for improving patient reported LBP outcomes.
Variation of Semidiurnal Internal Tides along the Southeastern Coast of Korea Induced by Typhoons
Authors: Chae Jeong-Yeob , Jeon Chanhyung , Kim Pyeongjoong , Hirose Naoki , Ku Ahyoung , Park Jae-Hun
Published: March 2021

Typhoon-induced strong winds can dramatically change the oceanic environment, occasionally resulting in sudden stratification changes. In July 2015, two consecutive typhoons, Chanhom and Nangka, passed over the Yellow and East/Japan Seas within a week. Remarkable temperature variations were observed near the southeastern coast of Korea, caused by typhoon-induced upwelling and downwelling events, which altered the energy of semidiurnal internal tides. During the typhoon-induced downwelling event, the energy of semidiurnal internal tides near the southeastern coast of Korea varied independently from the barotropic tidal forcing. Data-assimilated numerical simulation results reveal that the pycnocline, which is typically tilted toward the coast, enables the semidiurnal internal tidal energy to propagate toward shallow regions after being generated off the coast. Meanwhile, the downwelling event deepens the pycnocline near the coast and reflects and concentrates the semidiurnal internal tide energy near the bottom off the coast. A simple mechanism using the ratio between the wave characteristic slope and the bottom slope is proposed to explain the observed variations of semidiurnal internal tide energy near the coast. This paper demonstrates a case study showing that typhoon passage can modify the energetics of internal tides, which has the potential to cause unusual short-term coastal environmental changes.
Maternal Separation Followed by Chronic Mild Stress in Adulthood Is Associated with Concerted Epigenetic Regulation of AP-1 Complex Genes
Authors: Lundgaard Donovan Lene , Henningsen Kim , Flou Kristensen Anne , Wiborg Ove , Nieland John Dirk , Lichota Jacek
Published: March 2021

Depression is one of the most prevalent mental diseases worldwide. Patients with psychiatric diseases often have a history of childhood neglect, indicating that early-life experiences predispose to psychiatric diseases in adulthood. Two strong models were used in the present study: the maternal separation/early deprivation model (MS) and the chronic mild stress model (CMS). In both models, we found changes in the expression of a number of genes such as Creb and Npy. Strikingly, there was a clear regulation of expression of four genes involved in the AP-1 complex: c-Fos, c-Jun, FosB, and Jun-B. Interestingly, different expression levels were observed depending on the model, whereas the combination of the models resulted in a normal level of gene expression. The effects of MS and CMS on gene expression were associated with distinct histone methylation/acetylation patterns of all four genes. The epigenetic changes, like gene expression, were also dependent on the specific stressor or their combination. The obtained results suggest that single life events leave a mark on gene expression and the epigenetic signature of gene promoters, but a combination of different stressors at different life stages can further change gene expression through epigenetic factors, possibly causing the long-lasting adverse effects of stress.
Does Timing in Acquisition Modulate Heritage Children’s Language Abilities? Evidence from the Greek LITMUS Sentence Repetition Task
Authors: Makrodimitris Christos , Schulz Petra
Published: March 2021

Recent proposals suggest that timing in acquisition, i.e., the age at which a phenomenon is mastered by monolingual children, influences acquisition of the L2, interacting with age of onset of bilingualism and amount of L2 input. Here, we examine whether timing affects acquisition of the bilingual child’s heritage language, possibly modulating the effects of environmental and child-internal factors. The performance of 6- to 12-year-old Greek heritage children residing in Germany (age of onset of German: 0–4 years) was assessed across a range of nine syntactic structures via the Greek LITMUS (Language Impairment Testing in Multilingual Settings) Sentence Repetition Task. Based on previous studies on monolingual Greek, the structures were classified as “early” (main clauses (SVO), coordination, clitics, complement clauses, sentential negation, non-referential wh-questions) or as “late” (referential wh-questions, relatives, adverbial clauses). Current family use of Greek and formal instruction in Greek (environmental), chronological age, and age of onset of German (child-internal) were assessed via the Questionnaire for Parents of Bilingual Children (PABIQ); short-term memory (child-internal) was measured via forward digit recall. Children’s scores were generally higher for early than for late acquired structures. Performance on the three early structures with the highest scores was predicted by the amount of current family use of Greek. Performance on the three late structures was additionally predicted by forward digit recall, indicating that higher short-term memory capacity is beneficial for correctly reconstructing structurally complex sentences. We suggest that the understanding of heritage language development and the role of child-internal and environmental factors will benefit from a consideration of timing in the acquisition of the different structures.
Effect of Enamel Matrix Derivatives on Osteoclast Formation from PBMC of Periodontitis Patients and Healthy Individuals after Interaction with Activated Endothelial Cells
Authors: Durstberger Gerlinde , Nguyen Phuong Quynh , Hohensinner Verena , Pietschmann Peter , Rausch-Fan Xiaohui , Andrukhov Oleh
Published: March 2021

Background and objectives: Enamel matrix derivative (EMD) is produced from developing porcine tooth buds and represents a complex of low-molecular-weight hydrophobic enamel proteins. EMD is widely applied in periodontal regeneration. Osteoclasts are multinuclear cells, which are responsible for bone resorption. The precursors of osteoclasts, hematopoietic cells, undergo in vivo the process of transendothelial migration before differentiation. EMD is known to affect the process of osteoclastogenesis, but its effect on human osteoclasts precursors after the interaction with activated endothelium was never studied. Materials and Methods: Human umbilical vein endothelial cells (HUVECs)s were seeded in transwell inserts with a pore size of 8 µm and pre-activated by TNF-α and IL-1β for 18 h. Peripheral blood mononuclear cells (PBMCs), freshly isolated from 16 periodontitis patients and 16 healthy individuals, were added to pre-activated HUVECs. Adherent, non-adherent and transmigrated cells were collected and differentiated to osteoclasts by the standard protocol in the presence or absence of EMD. The number of osteoclasts was determined by tartrate-resistant acid phosphatase staining. Results: PBMCs isolated from periodontitis patients have formed a significantly higher osteoclast number compared to PBMCs isolated from healthy individuals (p < 0.05). EMD induced concentration-dependent inhibition of osteoclast formation from PBMCs. This was true for the different PBMC fractions isolated from both healthy individuals and periodontitis patients. Conclusions: Our data show that EMD inhibits the formation and activity of osteoclasts differentiated from the progenitor cells after the interaction with activated endothelium. This might be associated with bone resorption inhibition and supporting bone regeneration in the frame of periodontal therapy.
Oral and Maxillo-Facial Manifestations of Systemic Diseases: An Overview
Authors: Capodiferro Saverio , Limongelli Luisa , Favia Gianfranco
Published: March 2021

Many systemic (infective, genetic, autoimmune, neoplastic) diseases may involve the oral cavity and, more generally, the soft and hard tissues of the head and neck as primary or secondary localization. Primary onset in the oral cavity of both pediatric and adult diseases usually represents a true challenge for clinicians; their precocious detection is often difficult and requires a wide knowledge but surely results in the early diagnosis and therapy onset with an overall better prognosis and clinical outcomes. In the current paper, as for the topic of the current Special Issue, the authors present an overview on the most frequent clinical manifestations at the oral and maxillo-facial district of systemic disease.
A Rare Cause of Refractory Severe Polyhydramnios: Antenatal Bartter Syndrome
Authors: Nam Gina , Cho Angela , Park Mi-Hye
Published: March 2021

Background: Antenatal Bartter syndrome is an autosomal recessive disorder causing severe polyuria that leads to severe polyhydramnios and preterm labor. Prenatal diagnosis of antenatal Bartter syndrome is difficult because the genetic diagnosis can only be confirmed following a clinical diagnosis in infants. Reports of prenatal diagnosis and treatment of antenatal Bartter syndrome are limited. Case Presentation: We present the case of a 33-year-old pregnant woman with refractory polyhydramnios at 31 weeks of gestation. There were no structural anomalies or placental problems on ultrasonography; therefore, antenatal Bartter syndrome was suspected. With repeated amniocentesis and indomethacin therapy, the pregnancy continued to 36 weeks of gestation. The clinical features of the infant and subsequent genetic testing confirmed the diagnosis of antenatal Bartter syndrome. The baby was in good clinical condition at the 3-month follow-up visit. Conclusions: For pregnant women with early onset and refractory severe polyhydramnios without morphological anomalies, antenatal Bartter syndrome should be highly suspected.
Stability of Monolithic MOF Thin Films in Acidic and Alkaline Aqueous Media
Authors: Hashem Tawheed , Sanchez Elvia P. Valadez , Bogdanova Evgenia , Ugodchikova Anna , Mohamed Alaa , Schwotzer Matthias , Alkordi Mohamed H. , Wöll Christof
Published: March 2021

In the context of thin film nanotechnologies, metal-organic frameworks (MOFs) are currently intensively explored in the context of both, novel applications and as alternatives to existing materials. When it comes to applications under relatively harsh conditions, in several cases it has been noticed that the stability of MOF thin films deviates from the corresponding standard, powdery form of MOFs. Here, we subjected SURMOFs, surface-anchored MOF thin films, fabricated using layer-by layer methods, to a thorough characterization after exposure to different harsh aqueous environments. The stability of three prototypal SURMOFs, HKUST-1, ZIF-8, and UiO-66-NH2 was systematically investigated in acidic, neutral, and basic environments using X-ray diffraction and electron microscopy. While HKUST-1 films were rather unstable in aqueous media, ZIF-8 SURMOFs were preserved in alkaline environments when exposed for short periods of time, but in apparent contrast to results reported in the literature for the corresponding bulk powders- not stable in neutral and acidic environments. UiO-66-NH2 SURMOFs were found to be stable over a large window of pH values.
Microstructural Control of the Interface Layer for Strength Enhancement of Dissimilar Al/Cu Joints via Ni Addition during TIG Arc Brazing
Authors: Furuya Hiroki S. , Yabu Sakiko , Sato Yutaka S. , Kokawa Hiroyuki
Published: March 2021

Dissimilar metal joining between Al and Cu is effective for reducing the weight and cost of electrical components. In this study, dissimilar lap joining of pure Al to pure Cu with an Al-Ni filler material was conducted using tungsten inert gas (TIG) arc brazing, and the effect of Ni on the joint strength associated with the microstructure of the intermetallic compound (IMC) layer at the dissimilar interface was examined. The addition of Ni effectively increased the interfacial strength of the joints. Regardless of the addition of Ni, the joints fractured in the thick Al2Cu layer formed at the Al/Cu interface. However, the Ni addition reduced the thickness of the IMC layer and led to the formation of Al7Cu4Ni particles in the weakest Al2Cu layer. Both the thickness reduction and reinforcing Al7Cu4Ni particle formation are thought to contribute to the increase in joint strength of the Al/Cu dissimilar interface.
Diversity and Physiological Characteristics of Antarctic Lichens-Associated Bacteria
Authors: Noh Hyun-Ju , Park Yerin , Hong Soon Gyu , Lee Yung Mi
Published: March 2021

The diversity of lichen-associated bacteria from lichen taxa Cetraria, Cladonia, Megaspora, Pseudephebe, Psoroma, and Sphaerophorus was investigated by sequencing of 16S rRNA gene amplicons. Physiological characteristics of the cultured bacterial isolates were investigated to understand possible roles in the lichen ecosystem. Proteobacteria (with a relative abundance of 69.7–96.7%) were mostly represented by the order Rhodospirillales. The 117 retrieved isolates were grouped into 35 phylotypes of the phyla Actinobacteria (27), Bacteroidetes (6), Deinococcus-Thermus (1), and Proteobacteria (Alphaproteobacteria (53), Betaproteobacteria (18), and Gammaproteobacteria (12)). Hydrolysis of macromolecules such as skim milk, polymer, and (hypo)xanthine, solubilization of inorganic phosphate, production of phytohormone indole-3-acetic acid, and fixation of atmospheric nitrogen were observed in different taxa. The potential phototrophy of the strains of the genus Polymorphobacter which were cultivated from a lichen for the first time was revealed by the presence of genes involved in photosynthesis. Altogether, the physiological characteristics of diverse bacterial taxa from Antarctic lichens are considered to imply significant roles of lichen-associated bacteria to allow lichens to be tolerant or competitive in the harsh Antarctic environment.
Loop-Mediated Isothermal Amplification Allows Rapid, Simple and Accurate Molecular Diagnosis of Human Cutaneous and Visceral Leishmaniasis Caused by Leishmania infantum When Compared to PCR
Authors: Ibarra-Meneses Ana Victoria , Chicharro Carmen , Sánchez Carmen , García Emilia , Ortega Sheila , Ndung’u Joseph Mathu , Moreno Javier , Cruz Israel , Carrillo Eugenia
Published: March 2021

Loop-mediated isothermal amplification allows the rapid, sensitive and specific amplification of DNA without complex and expensive equipment. We compared the diagnostic performance of Loopamp™ Leishmania Detection Kit (Eiken Chemical Co., Ltd., Tokyo, Japan) with conventional and real-time polymerase chain reaction (PCR) for human cutaneous and visceral leishmaniasis caused by L. infantum. A total of 230 DNA samples from cutaneous (CL) and visceral (VL) leishmaniasis cases and controls from Spain, characterized by Leishmania nested PCR (LnPCR) were tested by: (i) the Loopamp™ Leishmania Detection Kit (Loopamp), run on Genie III real-time fluorimeter (OptiGene, UK); and (ii) real-time quantitative PCR (qPCR). The Loopamp test returned 98.8% (95% confidence interval—CI: 96.0–100.00) sensitivity and specificity of 97.7% (95% CI: 92.2–100) on VL samples, and 100% (95% CI: 99.1–100) sensitivity and 100.0% (95% CI: 98.8–100.0) specificity on CL samples. The Loopamp time-to-positivity (Tp) obtained by real-time fluorimetry showed excellent concordance (C = 97.91%) and strong correlation (r = 0.799) with qPCR’s cycle threshold (Ct). The performance of Loopamp is comparable to that of LnPCR and qPCR in the diagnosis of cutaneous and visceral leishmaniasis due to L. infantum. The excellent correlation between the Tp and Ct should be further investigated to determine the accuracy of Loopamp to quantify parasite load in tissues.
DNA-Mediated Stack Formation of Nanodiscs
Authors: Subramanian Madhumalar , Kielar Charlotte , Tsushima Satoru , Fahmy Karim , Oertel Jana
Published: March 2021

Membrane-scaffolding proteins (MSPs) derived from apolipoprotein A-1 have become a versatile tool in generating nano-sized discoidal membrane mimetics (nanodiscs) for membrane protein research. Recent efforts have aimed at exploiting their controlled lipid protein ratio and size distribution to arrange membrane proteins in regular supramolecular structures for diffraction studies. Thereby, direct membrane protein crystallization, which has remained the limiting factor in structure determination of membrane proteins, would be circumvented. We describe here the formation of multimers of membrane-scaffolding protein MSP1D1-bounded nanodiscs using the thiol reactivity of engineered cysteines. The mutated positions N42 and K163 in MSP1D1 were chosen to support chemical modification as evidenced by fluorescent labeling with pyrene. Minimal interference with the nanodisc formation and structure was demonstrated by circular dichroism spectroscopy, differential light scattering and size exclusion chromatography. The direct disulphide bond formation of nanodiscs formed by the MSP1D1_N42C variant led to dimers and trimers with low yield. In contrast, transmission electron microscopy revealed that the attachment of oligonucleotides to the engineered cysteines of MSP1D1 allowed the growth of submicron-sized tracts of stacked nanodiscs through the hybridization of nanodisc populations carrying complementary strands and a flexible spacer.
Determination of Toxic Pyrrolizidine Alkaloids in Traditional Chinese Herbal Medicines by UPLC-MS/MS and Accompanying Risk Assessment for Human Health
Authors: Wang Junchi , Zhang Meng , Chen Lihua , Qiao Yue , Ma Siqi , Sun Dian , Si Jianyong , Liao Yonghong
Published: March 2021

Pyrrolizidine alkaloids (PAs) are a class of natural toxins with hepatotoxicity, genotoxicity and carcinogenicity. They are endogenous and adulterated toxic components widely found in food and herbal products. In this study, a sensitive and efficient ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was used to detect the PAs in 386 kinds of Chinese herbal medicines recorded in the Chinese Pharmacopoeia (2020). The estimated daily intake (EDI) of 0.007 μg/kg body weight (bw)/day was adopted as the safety baseline. The margin of exposure (MOE) approach was applied to evaluate the chronic exposure risk for the genotoxic and carcinogenic potential of PAs. Results showed that PAs was detected in 271 out of 386 samples with a content of 0.1–25,567.4 μg/kg, and there were 20 samples with EDI values above the baseline, 0.007 μg/kg bw/day. Beyond that, the MOE values for 10 out of 271 positive samples were below 10,000. Considering the actual situation, Haber’s rule was used to assume two weeks exposure every year during lifetime, and still the MOE values for four out of 271 positive samples were under 10,000, indicating these products may have potential health risk. The developed method was successfully applied to detect the PAs-containing Chinese herbal medicines. This study provides convincing data that can support risk management actions in China and a meaningful reference for the rational and safe use of Chinese herbal medicines.
Retaining Skin Barrier Function Properties of the Stratum Corneum with Components of the Natural Moisturizing Factor—A Randomized, Placebo-Controlled Double-Blind In Vivo Study
Authors: Schleusener Johannes , Salazar Andrew , von Hagen Jörg , Lademann Jürgen , Darvin Maxim E.
Published: March 2021

The influence of a topically applied formulation containing components of natural moisturizing factor (NMF) on barrier-related parameters of the stratum corneum (SC) was investigated in vivo using confocal Raman microspectroscopy in a randomized, placebo-controlled double-blind study on 12 volunteers for 14 days. This method allowed for the elucidation of subtle differences between the verum and the placebo even though the components of the verum naturally occur in the SC. This differentiation is not possible non-invasively by conventional methods. In this study, we found that the applied verum and placebo formulations disrupted the equilibrium of water, NMF and lipids in the SC. The adverse effects of the formulation could be mitigated by incorporating it into a simplified supplementation of NMF molecules. As a long-term effect, the amount of strongly bound water increases at 30–40% SC depth (p < 0.05) and the amount of weakly bound water decreases at 30–40% SC depth (p < 0.05) for the verum. This supplement was also unexpectedly able to prevent intercellular lipids (ICL) disorganization in selected depths. In the long term, the verum treatment limited the lateral disorganization of the ICL to the upper 20% SC depth. Further research is required to elucidate the interplay of these factors in the SC, to better understand their contribution to the equilibrium and barrier function of the skin. This understanding of the interaction of these naturally occurring components could help in the future to develop and optimize topical treatments for diseases like psoriasis, atopic dermatitis, ichthyosis where the skin barrier is disrupted.
Ugi Reaction on α-Phosphorated Ketimines for the Synthesis of Tetrasubstituted α-Aminophosphonates and Their Applications as Antiproliferative Agents
Authors: López-Francés Adrián , del Corte Xabier , Martínez de Marigorta Edorta , Palacios Francisco , Vicario Javier
Published: March 2021

An Ugi three-component reaction using preformed α-phosphorated N-tosyl ketimines with different isocyanides in the presence of a carboxylic acid affords tetrasubstituted α-aminophosphonates. Due to the high steric hindrance, the expected acylated amines undergo a spontaneous elimination of the acyl group. The reaction is applicable to α-aryl ketimines bearing a number of substituents and several isocyanides. In addition, the densely substituted α-aminophosphonate substrates showed in vitro cytotoxicity, inhibiting the growth of carcinoma human tumor cell line A549 (carcinomic human alveolar basal epithelial cell).
CuBi2O4 Synthesis, Characterization, and Application in Sensitive Amperometric/Voltammetric Detection of Amoxicillin in Aqueous Solutions
Authors: Dumitru (m.Vodă) Raluca , Negrea Sorina , Păcurariu Cornelia , Surdu Adrian , Ianculescu Adelina , Pop Aniela , Manea Florica
Published: March 2021

CuBi2O4 synthesized by thermolysis of a new Bi(III)-Cu(II) oxalate coordination compound, namely Bi2Cu(C2O4)4·0.25H2O, was tested through its integration within carbon nanofiber paste electrode, namely CuBi/carbon nanofiber (CNF), for the electrochemical detection of amoxicillin (AMX) in the aqueous solution. Thermal analysis and IR spectroscopy were used to characterize a CuBi2O4 precursor to optimize the synthesis conditions. The copper bismuth oxide obtained after a heating treatment of the precursor at 700 °C/1 h was investigated by an X-ray diffraction and scanning electron microscopy. The electrochemical behavior of CuBi/CNF in comparison with CNF paste electrode showed the electrocatalytic activity of CuBi2O4 toward amoxicillin detection. Two potential detections, with one at the potential value of +0.540 V/saturated calomel electrode (SCE) and the other at the potential value of −1.000 V/SCE, were identified by cyclic voltammetry, which were exploited to develop the enhanced voltammetric and/or amperometric detection protocols. Better electroanalytical performance for AMX detection was achieved for CuBi/CNF using differential-pulsed and square-wave voltammetries than others reported in the literature. Very nice results obtained through anodic and cathodic currents recorded at +0.750 V/SCE and −1.000 V/SCE in the same time period using a pseudo multiple-pulsed amperometry technique showed the great potential of the CuBi/CNF paste electrode for practical applications in amoxicillin detection in aqueous solutions.
Occupational Exposure to Carbon Nanotubes and Carbon Nanofibres: More Than a Cobweb
Authors: Bergamaschi Enrico , Garzaro Giacomo , Wilson Jones Georgia , Buglisi Martina , Caniglia Michele , Godono Alessandro , Bosio Davide , Fenoglio Ivana , Guseva Canu Irina
Published: March 2021

Carbon nanotubes (CNTs) and carbon nanofibers (CNFs) are erroneously considered as singular material entities. Instead, they should be regarded as a heterogeneous class of materials bearing different properties eliciting particular biological outcomes both in vitro and in vivo. Given the pace at which the industrial production of CNTs/CNFs is increasing, it is becoming of utmost importance to acquire comprehensive knowledge regarding their biological activity and their hazardous effects in humans. Animal studies carried out by inhalation showed that some CNTs/CNFs species can cause deleterious effects such as inflammation and lung tissue remodeling. Their physico-chemical properties, biological behavior and biopersistence make them similar to asbestos fibers. Human studies suggest some mild effects in workers handling CNTs/CNFs. However, owing to their cross-sectional design, researchers have been as yet unable to firmly demonstrate a causal relationship between such an exposure and the observed effects. Estimation of acceptable exposure levels should warrant a proper risk management. The aim of this review is to challenge the conception of CNTs/CNFs as a single, unified material entity and prompt the establishment of standardized hazard and exposure assessment methodologies able to properly feed risk assessment and management frameworks.
Hierarchical HZSM-5 for Catalytic Cracking of Oleic Acid to Biofuels
Authors: Arumugam Mahashanon , Goh Chee Keong , Zainal Zulkarnain , Triwahyono Sugeng , Lee Adam F. , Wilson Karen , Taufiq-Yap Yun Hin
Published: March 2021

Solid acid catalyzed cracking of waste oil-derived fatty acids is an attractive route to hydrocarbon fuels. HZSM-5 is an effective acid catalyst for fatty acid cracking; however, its microporous nature is susceptible to rapid deactivation by coking. We report the synthesis and application of hierarchical HZSM-5 (h-HZSM-5) in which silanization of pre-crystallized zeolite seeds is employed to introduce mesoporosity during the aggregation of growing crystallites. The resulting h-HZSM-5 comprises a disordered array of fused 10–20 nm crystallites and mesopores with a mean diameter of 13 nm, which maintain the high surface area and acidity of a conventional HZSM-5. Mesopores increase the yield of diesel range hydrocarbons obtained from oleic acid deoxygenation from ~20% to 65%, attributed to improved acid site accessibility within the hierarchical network.
Biological Applications of Severely Plastically Deformed Nano-Grained Medical Devices: A Review
Authors: Kalantari Katayoon , Saleh Bahram , Webster Thomas J.
Published: March 2021

Metallic materials are widely used for fabricating medical implants due to their high specific strength, biocompatibility, good corrosion properties, and fatigue resistance. Recently, titanium (Ti) and its alloys, as well as stainless steel (SS), have attracted attention from researchers because of their biocompatibility properties within the human body; however, improvements in mechanical properties while keeping other beneficial properties unchanged are still required. Severe plastic deformation (SPD) is a unique process for fabricating an ultra-fine-grained (UFG) metal with micrometer- to nanometer-level grain structures. SPD methods can substantially refine grain size and represent a promising strategy for improving biological functionality and mechanical properties. This present review paper provides an overview of different SPD techniques developed to create nano-/ultra-fine-grain-structured Ti and stainless steel for improved biomedical implant applications. Furthermore, studies will be covered that have used SPD techniques to improve bone cell proliferation and function while decreasing bacterial colonization when cultured on such nano-grained metals (without resorting to antibiotic use).
Nitrogen Pools in Tropical Plantations of N2-Fixing and Non-N2-Fixing Legume Trees under Different Tree Stand Densities
Authors: Bighi Kelly Nery , Paula Ranieri Ribeiro , Caldeira Marcos Vinícius Winckler , Burak Diego Lang , Mendonça Eduardo de Sá , de Souza Paulo Henrique , Delarmelina William Macedo , Balieiro Fabiano de Carvalho
Published: March 2021

We investigated the nitrogen pools in monocultures of legume species widely used in reforestation in Brazil that have contrasting growth and nitrogen acquisition strategies. The plantations were established with the slow-growing and N2-fixing tree Anadenanthera peregrina var. peregrina, and the fast-growing and non-fixing tree Schizolobium parahyba var. amazonicum. The measurements of N pools in the tree biomass and the soil followed standard methods and were carried out on 54 experimental plots. The N2 fixation pools were evaluated by abundance natural of 15N and the N accretion methods. The soil N content was of similar magnitude between species and stand densities. The species showed similar amounts of N in the biomass, but divergent patterns of N accumulation, as well as the 15N signature on the leaves. S. parahyba accumulated most N in the stem, while A. peregrina accumulated N in the roots and leaves. However, the N accumulation in biomass of A. peregrina stand was less constrained by environment than in S. parahyba stands. The percentage of N derived from N2 fixation in A. peregrina stands decreased with the increase of stand density. The biological N2 fixation estimates depended on the method and the response of tree species to environment.
Mediterranean Water Properties at the Eastern Limit of the North Atlantic Subtropical Gyre since 1981
Authors: Frazão Helena C. , Waniek Joanna J.
Published: March 2021

A high-quality hydrographic CTD and Argo float data was used to study the property changes along the westward branch of the Mediterranean Outflow Water (MOW) in the northeast Atlantic between 1981 and 2018. In this period, the temperature and salinity are marked by periods of cooling/freshening and warming/salinification. Since 1981, the MOW properties at the core decreased by −0.015 ± 0.07 °C year−1 and −0.003 ± 0.002 year−1. The different phases of the North Atlantic Oscillation (NAO) influence the main propagation pathways of the MOW into the North Atlantic basin, thus affecting the trends determined within different NAO-phases. The temperature and salinity show a strong correlation with NAO, with NAO leading the properties by 8 and 7 years, respectively, indicating a delayed response of the ocean to different forcing conditions. A decrease in oxygen concentration (−0.426 ± 0.276 μmol kg−1 year−1) was calculated for the same period; however, no connection with the NAO was found.
Prophylactic Corneal Cross-Linking in Myopic Femtosecond Laser-Assisted In Situ Keratomileusis: Long-Term Visual and Refractive Outcomes
Authors: Alonso-Aliste Federico , Amián-Cordero Jonatan , Rachwani-Anil Rahul , De-Hita-Cantalejo Concepción , Borroni Davide , Rocha-de-Lossada Carlos , Sánchez-González José-María
Published: March 2021

The purpose of our study was to evaluate the safety, effectiveness, predictability, and stability of myopic and astigmatic laser-assisted in situ keratomileusis (LASIK) with simultaneous prophylactic corneal cross-linking (CXL) in thin corneas. In total, 100 eyes from 50 patients who were subjected to myopic and astigmatism femtosecond LASIK with simultaneous prophylactic CXL were included. The design of the study was retrospective, longitudinal, and observational. All patients had a 48-month follow-up. The MEL 80 excimer laser was utilized with the Aberration Smart Ablation platform. CXL treatment was applied when the predicted stromal thickness was less than 330 µm. Patients’ mean age was 30.22 ± 5.97 years. Previous mean spherical equivalent was −5.50 ± 1.65 (−9.50 to −1.13) diopters (D). Postoperative mean spherical equivalent was −0.24 ± 0.29 (−0.85 to +0.50) D. Visual acuity (VA) of 20/20 or better was observed in 87% of the eyes and no eyes experienced VA loss. Spherical equivalent within ±0.50 D was observed in 93% of eyes, and 4% of eyes varied by 0.50 D or more between 3 and 48 months. Prophylactic corneal cross-linking with simultaneous femtosecond laser-assisted in situ keratomileusis in thin corneas proved to be effective, safe, and predictable. The results remained stable after 48 months of follow-up.
Should Veterinary Practitioners Be Concerned about Acanthamoeba Keratitis?
Authors: Cooper Elise , Cowmeadow William , Elsheikha Hany M.
Published: March 2021

When presented with an animal exhibiting signs of keratitis (inflammation of the cornea), such as impaired vision, mucoid discharges, redness, swelling, and corneal oedema, most veterinarians would think of bacteria, viruses, or fungi as the potential causative agent(s). However, evidence has arisen in recent years of a possible connection between the protozoan Acanthamoeba and keratitis in animals. Acanthamoeba infection is underdiagnosed, but potentially common, in animals. In view of the paucity of evidence regarding Acanthamoeba keratitis (AK) in animals, this review has been written to increase awareness of veterinary professionals of the potential threat associated with AK and to stimulate more studies into the extent of occurrence and clinical impact of AK in animals.
Distribution of CRISPR Types in Fluoroquinolone-Resistant Campylobacter jejuni Isolates
Authors: Adiguzel Mehmet Cemal , Goulart Debora Brito , Wu Zuowei , Pang Jinji , Cengiz Seyda , Zhang Qijing , Sahin Orhan
Published: March 2021

To aid development of phage therapy against Campylobacter, we investigated the distribution of the clustered regularly interspaced short palindromic repeats (CRISPR) systems in fluoroquinolone (FQ)-resistant Campylobacter jejuni. A total of 100 FQ-resistant C. jejuni strains from different sources were analyzed by PCR and DNA sequencing to determine resistance-conferring mutation in the gyrA gene and the presence of various CRISPR systems. All but one isolate harbored 1–5 point mutations in gyrA, and the most common mutation was the Thr86Ile change. Ninety-five isolates were positive with the CRISPR PCR, and spacer sequences were found in 86 of them. Among the 292 spacer sequences identified in this study, 204 shared 93–100% nucleotide homology to Campylobacter phage D10, 44 showed 100% homology to Campylobacter phage CP39, and 3 had 100% homology with Campylobacter phage CJIE4-5. The remaining 41 spacer sequences did not match with any phages in the database. Based on the results, it was inferred that the FQ-resistant C. jejuni isolates analyzed in this study were potentially resistant to Campylobacter phages D10, CP39, and CJIE4-5 as well as some unidentified phages. These phages should be excluded from cocktails of phages that may be utilized to treat FQ-resistant Campylobacter.
Trematode Proteomics: Recent Advances and Future Directions
Authors: Bennett Adam P. S. , Robinson Mark W.
Published: March 2021

Trematodes cause disease in millions of people worldwide, but the absence of commercial vaccines has led to an over-reliance on a handful of monotherapies to control infections. Since drug-resistant fluke populations are emerging, a deeper understanding of parasite biology and host interactions is required to identify new drug targets and immunogenic vaccine candidates. Mass spectrometry-based proteomics represents a key tool to that end. Recent studies have capitalised on the wider availability of annotated helminth genomes to achieve greater coverage of trematode proteomes and discover new aspects of the host–parasite relationship. This review focusses on these latest advances. These include how the protein components of fluke extracellular vesicles have given insight into their biogenesis and cellular interactions. In addition, how the integration of transcriptome/proteome datasets has revealed that the expression and secretion of selected families of liver fluke virulence factors and immunomodulators are regulated in accordance with parasite development and migration within the mammalian host. Furthermore, we discuss the use of immunoproteomics as a tool to identify vaccine candidates associated with protective antibody responses. Finally, we highlight how established and emerging technologies, such as laser microdissection and single-cell proteomics, could be exploited to resolve the protein profiles of discrete trematode tissues or cell types which, in combination with functional tools, could pinpoint optimal targets for fluke control.
Modeling of the Refractive Index for the Systems MX+H2O, M2X+H2O, H3BO3+MX+H2O, and H3BO3+M2X+H2O. M = K+, Na+, or Li+ and X = Cl− or SO42−
Authors: Alavia Wilson , Soto Ismael , Lovera Jorge A.
Published: March 2021

The modeling of the refractive index for binary aqueous solutions of boric acid, sodium chloride, potassium chloride, sodium sulfate, lithium sulfate, and potassium sulfate, as well as ternary aqueous solutions of boric acid in the presence of sodium sulfate, lithium sulfate, or potassium chloride, is reported. The refraction index was represented by molar refraction. It was described as the sum of solutes’ partial molar refraction and solvent molar refraction. The solutes’ partial molar refraction was estimated from the molar refraction of the binary solutions. The excess molar refraction for these systems was described with the equation of Wang et al. The polarizability of the solutes present in the studied systems was estimated using the Lorenz–Lorenz relation. The results showed the model is appropriate for describing the systems studied; the interactions of boric acid, sodium, potassium, lithium, chloride, and sulfate ions with water molecules are relevant to explain the molar refraction and refractive index, and those for the binary systems of lithium chloride and sodium chloride are also relevant the ion–ion interactions. The model is robust and presents estimation capabilities within and beyond the concentrations and temperature range studied. Therefore, the outcomes represent valuable information to understand and follow the industrial processing of natural brines.
The New Elderly Patient: A Necessary Upgrade
Authors: Catapano Santo , Ortensi Luca , Mobilio Nicola , Grande Francesco
Published: March 2021

The elderly individual is becoming the most common patient in clinical practice due to the increasing average life span, especially in developed countries. The current elderly patients are different from those of some decades ago. They usually have an active social life, want to be informed, and are actively involved in the current society with consequent high expectations for medical and dental treatment. However, not all the elderly patients are like this. Some of them show limited financial resources, reduced mental and manual skills, and poor motivation. The purpose of this communication is to make a brief characterization of the new elderly population in view of prosthetic and dental management.
The Pioneering Role of Sci in Post Publication Public Peer Review (P4R)
Authors: Abdin Ahmad Yaman , Nasim Muhammad Jawad , Ney Yannick , Jacob Claus
Published: March 2021

Scientists observe, discover, justify and eventually share their findings with the scientific community. Dissemination is an integral aspect of scientific discovery, since discoveries which go unnoticed have no or little impact on science. Today, peer review is part of this process of scientific dissemination as it contributes proactively to the quality of a scientific article. As the numbers of scientific journals and scientific articles published therein are increasing steadily, processes such as the single-blind or double-blind peer review are facing a near collapse situation. In fact, these traditional forms of reviewing have reached their limits and, because of this, are also increasingly considered as unfair, sloppy, superficial and even biased. In this manuscript, we propose forms of post-publication public peer review (P4R) as valuable alternatives to the traditional blind peer review system. We describe how the journal Sci has explored such an approach and provide first empirical evidence of the benefits and also challenges, such a P4R approach faces.
Correction: Ritt, G. Laser Safety Calculations for Imaging Sensors. Sensors 2019, 19, 3765
Authors: Ritt Gunnar
Published: March 2021

The author wishes to make the following corrections to the paper [...]
Developing Industrial CPS: A Multi-Disciplinary Challenge
Authors: Hoffmann Martin , Malakuti Somayeh , Grüner Sten , Finster Soeren , Gebhardt Jörg , Tan Ruomu , Schindler Thorsten , Gamer Thomas
Published: March 2021

Industrial Cyber–Physical System (CPS) is an emerging approach towards value creation in modern industrial production. The development and implementation of industrial CPS in real-life production are rewarding yet challenging. This paper aims to present a concept to develop, commercialize, operate, and maintain industrial CPS which can motivate the advance of the research and the industrial practice of industrial CPS in the future. We start with defining our understanding of an industrial CPS, specifying the components and key technological aspects of the industrial CPS, as well as explaining the alignment with existing work such as Industrie 4.0 concepts, followed by several use cases of industrial CPS in practice. The roles of each component and key technological aspect are described and the differences between traditional industrial systems and industrial CPS are elaborated. The multidisciplinary nature of industrial CPS leads to challenges when developing such systems, and we present a detailed description of several major sub-challenges that are key to the long-term sustainability of industrial CPS design. Since the research of industrial CPS is still emerging, we also discuss existing approaches and novel solutions to overcome these sub-challenges. These insights will help researchers and industrial practitioners to develop and commercialize industrial CPS.
Risk Analysis by a Probabilistic Model of the Measurement Process
Authors: Toczek Wojciech , Smulko Janusz
Published: March 2021

The aim of the article is presentation of the testing methodology and results of examination the probabilistic model of the measurement process. The case study concerns the determination of the risk of an incorrect decision in the assessment of the compliance of products by measurement. Measurand is characterized by the generalized Rayleigh distribution. The model of the measurement process was tested in parallel mode by six risk metrics. An undesirable effect in the reconstruction building block of the model was detected, consisting in the distortion of probability distribution at the edges of the measuring range. The paper gives guidelines on how to use the model, to obtain the analytical risk assessment consistent with the results of the Monte Carlo method. The study can be useful in product quality control, test design, and fault diagnosis.
Luminescence Sensing Method for Degradation Analysis of Bioactive Glass Fibers
Authors: Baranowska Agata , Kochanowicz Marcin , Wajda Aleksandra , Leśniak Magdalena , Żmojda Jacek M. , Miluski Piotr , Zgłobicka Izabela , Kurzydłowski Krzysztof J. , Dorosz Dominik
Published: March 2021

The effects of Sm3+ content on the optical properties and bioactivity of 13-93 bioactive glass were presented. Sm3+ doped glass fibers drawn from bioactive glass were analyzed in simulated body fluid (SBF) for the determination of ion release. Optical analysis of the Sm3+ ions in bioactive glass fibers was used for degradation monitoring. While the fibers were immersed in SBF solution, changes in their luminescence spectra under 405 nm laser excitation were measured continuously for 48 h. The morphology of the fibers after the immersion process was determined by SEM/EDS. It was shown that the proposed approach to the analysis of changes in Sm3+ ion luminescence is a sensitive method for the monitoring of degradation processes and the formation of hydroxycarbonate-apatite (HCA) layers on glass fiber surfaces. SEM/EDS measurements showed a significant deterioration on the surface of the fibers and the formation of HCA on 13-93_02Sm bioactive glass. The optical analysis of the time constant indicated that bioactive glass fibers doped with 2 %mol Sm3+ degrade at a rate almost five times slower than 13-93_02Sm.