bluuebunny/crossref_metadata_2025 huggingface dataset:
Global Dynamics and Optimal Control of a Fractional-Order SIV Epidemic Model with Nonmonotonic Occurrence Rate
Authors: Yan Juhui , Wu Wanqin , Miao Qing , Tan Xuewen
Published: September 2024

This paper performs a detailed analysis and explores optimal control strategies for a fractional-order SIV epidemic model, incorporating a nonmonotonic incidence rate. In this paper, the population of vaccinated individuals is included in the disease dynamics model. After proving the non-negative boundedness of the fractional-order SIV model, we focus on analyzing the equilibrium point characteristics of the model, delving into its existence, uniqueness, and stability analysis. In addition, our research includes formulating optimal control strategies specifically aimed at minimizing the number of infections while keeping costs as low as possible. To validate the theoretical findings and uncover the practical efficacy and prospects of control measures in mitigating epidemic spread, numerical simulations are performed.
A Conceptual Framework for Optimizing Performance in Sustainable Supply Chain Management and Digital Transformation towards Industry 5.0
Authors: Dossou Paul-Eric , Alvarez-de-los-Mozos Esther , Pawlewski Pawel
Published: September 2024

The economic growth of developed or emerging countries through globalization has prompted them to increase their supply chain performance. A large number of concepts, tools, and methodologies have been proposed in support of this performance improvement. They are mainly based on the use of classical optimization or enterprise modeling methods. However, environmental and social issues, not to mention digital transformation, are often ignored or not sufficiently integrated. Indeed, the world geopolitical situation, the increase in oil prices, and the commitment to protect our earth require the integration of sustainability aspects and Industry 4.0 concepts like digital twin and artificial intelligence in transforming the supply chain. This paper focuses on defining a conceptual framework to support sustainable supply chain management and digital transformation. It aims to exploit the sustainability and digital maturity of companies to transform their supply chains and enhance their performance to meet the challenges of Industry 5.0. Several practices related to sustainability, as well as two use cases on optimization and digital twin, are presented to illustrate this framework. Finally, based on the previous practices and use cases, an adapted framework for the supply chain manager to support the transition from Industry 4.0 to Industry 5.0 has been developed, as well as a performance dashboard.
Using Kan Extensions to Motivate the Design of a Surprisingly Effective Unsupervised Linear SVM on the Occupancy Dataset
Authors: Pugh Matthew , Grundy Jo , Cirstea Corina , Harris Nick
Published: September 2024

Recent research has suggested that category theory can provide useful insights into the field of machine learning (ML). One example is improving the connection between an ML problem and the design of a corresponding ML algorithm. A tool from category theory called a Kan extension is used to derive the design of an unsupervised anomaly detection algorithm for a commonly used benchmark, the Occupancy dataset. Achieving an accuracy of 93.5% and an ROCAUC of 0.98, the performance of this algorithm is compared to state-of-the-art anomaly detection algorithms tested on the Occupancy dataset. These initial results demonstrate that category theory can offer new perspectives with which to attack problems, particularly in making more direct connections between the solutions and the problem’s structure.
Innovative Solutions to the Fractional Diffusion Equation Using the Elzaki Transform
Authors: Noor Saima , Alrowaily Albandari W. , Alqudah Mohammad , Shah Rasool , El-Tantawy Samir A.
Published: September 2024

This study explores the application of advanced mathematical techniques to solve fractional differential equations, focusing particularly on the fractional diffusion equation. The fractional diffusion equation, used to simulate a range of physical and engineering phenomena, poses considerable difficulties when applied to fractional orders. Thus, by utilizing the mighty powers of fractional calculus, we employ the variational iteration method (VIM) with the Elzaki transform to produce highly accurate approximations for these specific differential equations. The VIM provides an iterative framework for refining solutions progressively, while the Elzaki transform simplifies the complex integral transforms involved. By integrating these methodologies, we achieve accurate and efficient solutions to the fractional diffusion equation. Our findings demonstrate the robustness and effectiveness of combining the VIM and the Elzaki transform in handling fractional differential equations, offering explicit functional expressions that are beneficial for theoretical analysis and practical applications. This research contributes to the expanding field of fractional calculus, providing valuable insights and useful tools for solving complex, nonlinear fractional differential equations across various scientific and engineering disciplines.
Novel Galectins Purified from the Sponge Chondrilla australiensis: Unique Structural Features and Cytotoxic Effects on Colorectal Cancer Cells Mediated by TF-Antigen Binding
Authors: Hayashi Ryuhei , Kamata Kenichi , Gerdol Marco , Fujii Yuki , Hayashi Takashi , Onoda Yuto , Kobayashi Nanae , Furushima Satoshi , Ishiwata Ryuya , Ohkawa Mayuka , Masuda Naoko , Niimi Yuka , Yamada Masao , Adachi Daisuke , Kawsar Sarkar M. A. , Rajia Sultana , Hasan Imtiaj , Padma Somrita , Chatterjee Bishnu Pada , Ise Yuji , Chida Riku , Hasehira Kayo , Miyanishi Nobumitsu , Kawasaki Tatsuya , Ogawa Yukiko , Fujita Hideaki , Pallavicini Alberto , Ozeki Yasuhiro
Published: September 2024

We here report the purification of a novel member of the galectin family, the β-galactoside-binding lectin hRTL, from the marine sponge Chondrilla australiensis. The hRTL lectin is a tetrameric proto-type galectin with a subunit molecular weight of 15.5 kDa, consisting of 141 amino acids and sharing 92% primary sequence identity with the galectin CCL from the congeneric species C. caribensis. Transcriptome analysis allowed for the identification of additional sequences belonging to the same family, bringing the total number of hRTLs to six. Unlike most other galectins, hRTLs display a 23 amino acid-long signal peptide that, according to Erdman degradation, is post-translationally cleaved, leaving an N-terminal end devoid of acetylated modifications, unlike most other galectins. Moreover, two hRTLs display an internal insertion, which determines the presence of an unusual loop region that may have important functional implications. The characterization of the glycan-binding properties of hRTL revealed that it had high affinity towards TF-antigen, sialyl TF, and type-1 N-acetyl lactosamine with a Galβ1-3 structure. When administered to DLD-1 cells, a colorectal carcinoma cell line expressing mucin-associated TF-antigen, hRTL could induce glycan-dependent cytotoxicity.
PM2.5 Induces Pyroptosis via Activation of the ROS/NF-κB Signaling Pathway in Bronchial Epithelial Cells
Authors: Kang Ji-Young , Choi Hyunsu , Oh Jeong-Min , Kim Minsu , Lee Dong-Chang
Published: September 2024

Background and Objectives: Fine particulate matter, PM2.5, is becoming a major threat to human health, particularly in terms of respiratory diseases. Pyroptosis is a recently discovered and distinct form of cell death, characterized by pore formation in the cell membrane and secretions of proinflammatory cytokines. There has been little research on the effect of PM2.5 on pyroptosis, especially in airway epithelium. We investigated whether PM2.5-related oxidative stress induces pyroptosis in bronchial epithelial cells and defined the underlying mechanisms. Materials and Methods: After exposure of a BEAS-2B cell line to PM2.5 concentration of 20 µg/mL, reactive oxygen species (ROS) levels, parameters related to pyroptosis, and NF-κB signaling were measured by Western blotting, immunofluorescence, and ELISA (Enzyme-linked immunosorbent assay). Results: PM2.5 induced pyroptotic cell death, accompanied by LDH (Lactate dehydrogenase) release and increased uptake of propidium iodide in a dose-dependent manner. PM2.5 activated the NLRP3-casp1-gasdermin D pathway, with resulting secretions of the proinflammatory cytokines IL-1β and IL-18. The pyroptosis activated by PM2.5 was alleviated significantly by NLRP3 inhibitor. In PM2.5-exposed BEAS-2B cells, levels of intracellular ROS and NF-κB p65 increased. ROS scavenger inhibited the expression of the NLRP3 inflammasome, and the NF-κB inhibitor attenuated pyroptotic cell death triggered by PM2.5 exposure, indicating that the ROS/NF-κB pathway is involved in PM2.5-induced pyroptosis. Conclusions: These findings show that PM2.5 exposure can cause cell injury by NLRP3-inflammasome-mediated pyroptosis by upregulating the ROS/NF-κB pathway in airway epithelium.
The Effect of Anesthesia Type on the Stability of the Surgical View on the Monitor in Retrograde Intrarenal Surgery for Renal Stone: A Prospective Observational Trial
Authors: Won Dongwook , Cho Sung Yong , No Hyun-Joung , Lee Jiwon , Hwang Jin-Young , Kim Tae Kyong , Chang Jee-Eun , Kim Hyerim , Choi Jae-Hyun , Lee Jung-Man
Published: September 2024

Background and Objectives: Retrograde intrarenal surgery (RIRS) is a minimally invasive technique for nephrolithiasis. RIRS is performed via a monitor screen displaying a magnified surgical site. Respiration can affect the stability of the surgical view during RIRS because the kidneys are close to the diaphragm. The purpose of this trial is to compare the effect of anesthesia type on the stability of the surgical view during RIRS between spinal anesthesia and general anesthesia. Materials and Methods: Patients were allocated to the general anesthesia group or spinal anesthesia group. During surgery, movement of the surgical field displayed on the monitor screen was graded by the first assistant on a 10-grade numeric rating scale (0–10). Next, it was also graded by the main surgeon. After surgery, we evaluated the discomfort with the anesthesia method for all patients. Results: Thirty-four patients were allocated to the general anesthesia group and 32 patients to the spinal anesthesia group. The average values of the two surgeons for surgical field oscillation grade showed vision on the monitor screen was more stable in the general anesthesia group than the spinal anesthesia group (3.3 ± 1.6 vs. 5.0 ± 1.6, p < 0.001). The degrees of the inconvenience of the surgery did not differ between the groups (0.7 ± 1.8 vs. 1.6 ± 2.6, p = 0.114), even though more patients reported inconvenience with a grade of 3 or more in the spinal anesthesia group (8.8% vs. 28.1%, p = 0.042). Conclusions: In terms of the visualization of the surgical site, general anesthesia might provide a more stable surgical view during RIRS compared to spinal anesthesia without increasing inconvenience induced by the type of anesthesia.
Evaluation of Amiodarone Administration in Patients with New-Onset Atrial Fibrillation in Septic Shock
Authors: Oprea Andreea , Marina Virginia , Ciobotaru Oana Roxana , Popescu Cristina-Mihaela
Published: September 2024

Background and Objective: New-onset atrial fibrillation (NOAF) is a common cardiac condition often observed in intensive care units. When amiodarone is used to treat this condition, either to maintain sinus rhythm after electrical cardioversion or to control heart rate, complications can arise when a systemic pathology is present. Systemic pathology can result in a decrease in cardiac output and blood pressure, making the management of NOAF and septic shock challenging. Limited international research exists on the coexistence of NOAF and septic shock, making it difficult to determine the optimal course of treatment. While amiodarone is not the primary choice of antiarrhythmic drug for patients in septic shock, it may be considered for those with underlying cardiac issues. This paper aims to investigate the safety of administering amiodarone to patients with septic shock and explore whether another antiarrhythmic drug may be more effective, especially considering the cardiac conditions that patients may have. Materials and Methods: To write this article, we searched electronic databases for studies where authors used amiodarone and other medications for heart rate control or sinus rhythm restoration. Results: The studies reviewed in this work have shown that for the patients with septic shock and NOAF along with a pre-existing cardiac condition like a dilated left atrium, the use of amiodarone may provide greater benefits compared to other antiarrhythmic drugs. For patients with NOAF and septic shock without underlying heart disease, the initial use of propafenone has been found to be advantageous. However, a challenge arises when deciding between rhythm or heart rate control using various drug classes. Unfortunately, there is limited literature available on this specific scenario. Conclusions: NOAF is a frequent and potentially life-threatening complication occurring in one out of seven patients with sepsis, and its incidence is rising among patients with septic shock.
Survival Analysis, Clinical Characteristics, and Predictors of Cerebral Metastases in Patients with Colorectal Cancer
Authors: Jeri-Yabar Antoine , Vittini-Hernandez Liliana , Benites-Meza Jerry K. , Prado-Nuñez Sebastian
Published: September 2024

Introduction: Colorectal cancer (CRC) is the third most common cancer globally and a leading cause of cancer-related deaths. While liver metastasis is common, brain metastasis (BM) is rare, occurring in 0.1% to 14% of cases. Risk factors for BM include lung metastasis at diagnosis, rectal cancer, and mutations in RAS and KRAS genes. Due to its rarity, guidelines for BM screening and treatment are limited. The aim of this study is to identify the clinical characteristics and predictors of BM at the time of the initial diagnosis of CRC. Methods: We evaluated patients ≥18 years old with metastatic colorectal cancer and brain metastases at diagnosis from the SEER database (2010–2021). A retrospective cohort study was conducted to analyze overall survival and predictive factors for brain metastasis, utilizing multivariate logistic regression, Kaplan–Meier survival analysis, and the Cox proportional hazards models, with p-values < 0.05 considered significant. Results: Out of 24,703 patients with metastatic colorectal cancer (mCRC), 228 (0.92%) had brain metastasis (BM) at diagnosis. BM was more prevalent in average-onset mCRC (≥50 years) compared to early-onset (<50 years) (1% vs. 0.55%, p = 0.004). Certain factors, such as older age and adenocarcinoma subtype, were associated with BM. Additionally, Asians/Pacific-Islanders (HR 1.83 CI: 1.01-3-33, p = 0.045) and American Indians/Alaska Natives (HR 4.79 CI 1.15–19.97, p = 0.032) had higher mortality rates, while surgical treatment and chemotherapy were linked to decreased mortality. Patients with BM had significantly worse overall survival (6 months vs. 21 months, p < 0.001). Conclusion: BM in mCRC is uncommon, but it is associated with significantly worse outcomes, including markedly reduced overall survival. Our study highlights several critical factors associated with the presence of BM, such as older age and specific racial/ethnic groups, which may inform risk stratification and early-detection strategies. Our findings emphasize the need for heightened awareness and screening for BM in high-risk mCRC patients, as well as the inclusion of these patients in clinical trials to explore tailored therapeutic approaches aimed at improving survival and quality of life.
Influence of Ceramic Size and Morphology on Interface Bonding Properties of TWIP Steel Matrix Composites Produced by Lost-Foam Casting
Authors: Sun Guojin , Liu Xiaoming , Li Zhenggui , Wang Qi
Published: September 2024

This study investigated the fabrication and characterization of large ceramic-reinforced TWIP (twinning-induced plasticity) steel matrix composites using the lost-foam casting technique. Various ceramic shapes and sizes, including blocky, flaky, rod-like, and granular forms, were evaluated for their suitability as reinforcement materials. The study found that rod-like and granular ceramics exhibited superior structural integrity and formed strong interfacial bonds with the TWIP steel matrix compared to blocky and flaky ceramics, which suffered from cracking and fragmentation. Detailed microstructural analysis using scanning electron microscopy (SEM) and industrial computed tomography (CT) revealed the mechanisms influencing the composite formation. The results demonstrated that rod-like and granular ceramics are better for reinforcing TWIP steel composites, providing excellent mechanical stability and enhanced performance. This work contributes to the development of advanced composite structures with potential applications in industries requiring high-strength and durable materials.
Potential Probiotic Properties and Complete Genome Analysis of Limosilactobacillus reuteri LRA7 from Dogs
Authors: Zhang Yuanyuan , Zhao Mengdi , Li Yueyao , Liang Shuang , Li Xinkang , Wu Yi , Li Guangyu
Published: September 2024

This study aimed to isolate and screen canine-derived probiotics with excellent probiotic properties. Strain characterization was conducted using a combination of in vitro and in vivo probiotic characterization and safety assessments, as well as complete genome analysis. The results showed that Limosilactobacillus reuteri LRA7 exhibited excellent bacteriostatic and antioxidant activities. The survival rate at pH 2.5 was 79.98%, and the viable counts after exposure to gastrointestinal fluid and 0.5% bile salts were 7.77 log CFU/mL and 5.29 log CFU/mL, respectively. The bacterium also exhibited high hydrophobicity, self-coagulation, and high temperature tolerance, was negative for hemolysis, and was sensitive to clindamycin. In vivo studies in mice showed that the serum superoxide dismutase activity level was 53.69 U/mL higher in the MR group of mice compared to that of the control group, the malondialdehyde content was 0.53 nmol/mL lower in the HR group, and the highest jejunal V/C value was 4.11 ± 1.05 in the HR group (p < 0.05). The L. reuteri LRA7 gene is 2.021 megabases in size, contains one chromosome and one plasmid, and is annotated with 1978 functional genes. In conclusion, L. reuteri LRA7 has good probiotic potential and is safe. It can be used as an ideal probiotic candidate strain of canine origin.
Cell Extracts Derived from Cypress and Cedar Show Antiviral Activity against Enveloped Viruses
Authors: Furukawa Takashi , Inagaki Ayumu , Hatta Takeshi , Moroishi Suzuha , Kawanishi Katsuki , Itoh Yuki , Maehana Shotaro , Amarasiri Mohan , Sei Kazunari
Published: September 2024

The antiviral efficacy of cell-extracts (CEs) derived from cypress (Chamaecyparis obtusa (Siebold & Zucc.) Endl., C. obtusa) and cedar (Cryptomeria japonica (Thunb. ex. L.) D.Don, C. japonica) was assessed using phi6 and MS2 bacteriophages, which are widely accepted surrogate models for enveloped and non-enveloped viruses, in order to verify their potential use as antiviral agents. Our results indicate that CEs derived from C. obtusa are dominantly composed of terpinen-4-ol (18.0%), α-terpinyl acetate (10.1%), bornyl acetate (9.7%), limonene (7.1%), and γ-terpinene (6.7%), while CEs derived from C. japonica are dominantly composed of terpinen-4-ol (48.0%) and α-pinene (15.9%), which exhibited robust antiviral activity against phi6 bacteriophage. Both CEs successfully inactivated the phi6 bacteriophage below the detection limit (10 PFU/mL) within a short exposure time of 30 s (log reduction value, LRV > 4). Through exposure experiments utilizing CEs with content ratios prepared via 2-fold serial dilutions (ranging from 3.13% to 100%), we demonstrated that the antiviral effect could be sustained up to a concentration of 25% (C. obtusa LRV = 3.8, C. japonica LRV > 4.3 at a 25% CE content ratio for each species). However, CEs with content ratios below 12.5% did not produce a significant reduction in bacteriophage concentration and consequently lost their antiviral effects. Conversely, both CEs did not exhibit antiviral activity against MS2 bacteriophage, a non-enveloped virus. Our findings reveal for the first time the potential of CEs derived from C. obtusa and C. japonica for use as antiviral agents specifically targeting enveloped viruses.
Dual-Emission Fluorescence Resonance Energy Transfer (FRET) PCR Discriminates Salmonella Pullorum and Gallinarum
Authors: Gong Jiansen , Iduu Nneka Vivian , Zhang Di , Chenoweth Kelly , Wei Lanjing , Yang Yi , Dou Xinhong , Wang Chengming
Published: September 2024

Salmonella Pullorum (S. Pullorum) and Salmonella Gallinarum (S. Gallinarum) are two biovars of Salmonella enterica serovar Gallinarum, responsible for pullorum disease and fowl typhoid, which are the most prevalent and pathogenic forms of salmonellosis in poultry in developing countries. Traditional differentiation methods for S. Pullorum and S. Gallinarum are based on distinct clinical manifestations and biochemical traits, given their indistinguishable nature via serological assays alone. Molecular differentiation methods such as allele-specific PCR and dual PCR combined with gel electrophoresis or enzyme digestion have also been used to discriminate S. Pullorum and S. Gallinarum, but the detection efficiency is not high. This investigation introduces a Fluorescence Resonance Energy Transfer (FRET) PCR assay targeting the pegB gene, exclusively found in specific Salmonella serovars such as S. Pullorum and S. Gallinarum, and exhibiting conserved single-nucleotide polymorphisms across these two biovars. High-resolution melting curve analysis demonstrates distinct dissolution profiles, facilitating the precise discrimination of S. Pullorum and S. Gallinarum. This FRET-PCR assay exhibits a detection limit of 10 copies per reaction and has been rigorously validated utilizing 17 reference strains and 39 clinical isolates. The innovation presented herein provides a valuable tool for the rapid differentiation of S. Pullorum and S. Gallinarum, thereby enhancing diagnostic efficiency and molecular surveillance of poultry Salmonella. The developed pegB-targeting FRET-PCR assay presents a promising alternative to current cumbersome and time-consuming diagnostic modalities, offering significant potential for expedited identification and control of Salmonella in poultry and mitigating economic losses associated with Salmonella contamination in poultry production.
Immobilization of Lipase from Thermomyces Lanuginosus and Its Glycerolysis Ability in Diacylglycerol Preparation
Authors: Xie Rui , Lee Yee-Ying , Xie Pengkai , Tan Chin-Ping , Wang Yong , Zhang Zhen
Published: September 2024

In the glycerolysis process for diacylglycerol (DAG) preparation, free lipases suffer from poor stability and the inability to be reused. To address this, a cost-effective immobilized lipase preparation was developed by cross-linking macroporous resin with poly (ethylene glycol) diglycidyl ether (PEGDGE) followed by lipase adsorption. The selected immobilization conditions were identified as pH 7.0, 35 °C, cross-linking agent concentration 2.0%, cross-linking time 4 h, lipase amount 5 mg/g of support, and adsorption time 4 h. Enzymatic properties of the immobilized lipase were analyzed, revealing enhanced pH stability, thermal stability, storage stability, and operational stability post-immobilization. The conditions for immobilized enzyme-catalyzed glycerolysis to produce DAG were selected, demonstrating the broad applicability of the immobilized lipase. The immobilized lipase catalyzed glycerolysis reactions using various oils as substrates, with DAG content in the products ranging between 35 and 45%, demonstrating broad applicability. Additionally, the changes during the repeated use of the immobilized lipase were characterized, showing that mechanical damage, lipase leakage, and alterations in the secondary structure of the lipase protein contributed to the decline in catalytic activity over time. These findings provide valuable insights for the industrial application of lipase.
High-Precision Determination of NMR Interaction Parameters by Measurement of Single Crystals: A Review of Classical and Advanced Methods
Authors: Bräuniger Thomas
Published: September 2024

In this review, the process of extracting precise values for NMR interaction tensors from single crystal samples is systematically explored. Starting with a description of the orientation dependence of the considered interactions, i.e., chemical shift, dipolar, and quadrupole interaction, the techniques for acquiring and analysing single-crystal spectra are outlined. This includes the ‘classical’ approach, which requires the acquisition of three rotation patterns around three rotation axes that are orthogonal to each other, as well as more recent strategies aimed at reducing the number of required NMR spectra. One such strategy is the ‘single-rotation method’, which exploits the symmetry relations between tensors in the crystal structure to reduce the necessary amount of orientation-dependent data. This concept may be extended to additionally include the orientation of the goniometer axis itself in the data fit, which may be termed the ‘minimal-rotation method’. Other, more exotic schemes, such as the use of specialised probe designs or the investigation of single crystals under magic-angle-spinning, are also briefly discussed. Actual values of NMR interaction tensors as determined from the various single-crystal methods have been collected and are provided in tables for spin I=1/2, I=1, and half-integer spins with I>1/2.
Preparation and Thermal Properties of Magnetic PW@CaCO3@Fe3O4 Phase-Change Microcapsules and Their Application to Textile Fabrics
Authors: Song Xiaolei , Xu Congzhu , Wei Hong , Li Yonggui , Sun Runjun , Wang Chunxia , Dong Jie , Feng Xinqun
Published: September 2024

Multifunctional thermal regulation materials with good thermal properties, efficient magnetic performance, and satisfactory interface bonding on fabrics are highly desirable for protective fabrics, building winter protection materials, medical thermal regulation materials, and special-environment work clothing. Herein, a new class of magnetic phase-change PW@CaCO3@Fe3O4 microcapsules was successfully produced by controlling the content of magnetic Fe3O4 through a self-assembly method. The microstructure, chemical composition, phase-change behavior, and magnetic properties of the products were sequentially characterized and analyzed. The findings revealed that the obtained microcapsules possessed regular spherical structure with uniform size and excellent thermal properties. Furthermore, PW@CaCO3 with Fe3O4 (i.e., 8% mass fraction) showed the highest thermal regulation and magnetic properties and reached an enthalpy value of 94.25 J·g−1, which is clearly superior to the value of 77.51 J·g−1 for PW@CaCO3 microcapsules. At the same time, the encapsulation efficiency of 38.7% and saturation magnetization of 2.50 emu·g−1 were the best among the four given samples. Therefore, the good paramagnetic feature had a significant synergistic effect on the thermal properties of the PW@CaCO3 microcapsules under study. More importantly, multifunctional fabrics loaded with PW@CaCO3@Fe3O4 microcapsules still showed an enthalpy value of 25.81 J·g−1 after several washes and have the potential to be used widely in the field of temperature control. The thermal regulation fabrics in this study exhibited excellent thermal properties and fastness, which contribute to their practical applications in advancing multifunctional textiles and high-technology modern fabrics.
Strategic Fluorination to Achieve a Potent, Selective, Metabolically Stable, and Orally Bioavailable Inhibitor of CSNK2
Authors: Ong Han Wee , Yang Xuan , Smith Jeffery L. , Taft-Benz Sharon , Howell Stefanie , Dickmander Rebekah J. , Havener Tammy M. , Sanders Marcia K. , Brown Jason W. , Couñago Rafael M. , Chang Edcon , Krämer Andreas , Moorman Nathaniel J. , Heise Mark , Axtman Alison D. , Drewry David H. , Willson Timothy M.
Published: September 2024

The host kinase casein kinase 2 (CSNK2) has been proposed to be an antiviral target against β-coronaviral infection. To pharmacologically validate CSNK2 as a drug target in vivo, potent and selective CSNK2 inhibitors with good pharmacokinetic properties are required. Inhibitors based on the pyrazolo[1,5-a]pyrimidine scaffold possess outstanding potency and selectivity for CSNK2, but bioavailability and metabolic stability are often challenging. By strategically installing a fluorine atom on an electron-rich phenyl ring of a previously characterized inhibitor 1, we discovered compound 2 as a promising lead compound with improved in vivo metabolic stability. Compound 2 maintained excellent cellular potency against CSNK2, submicromolar antiviral potency, and favorable solubility, and was remarkably selective for CSNK2 when screened against 192 kinases across the human kinome. We additionally present a co-crystal structure to support its on-target binding mode. In vivo, compound 2 was orally bioavailable, and demonstrated modest and transient inhibition of CSNK2, although antiviral activity was not observed, possibly attributed to its lack of prolonged CSNK2 inhibition.
Resurgence of Clinical Malaria in Ethiopia and Its Link to Anopheles stephensi Invasion
Authors: Zhou Guofa , Taffese Hiwot S. , Zhong Daibin , Wang Xiaoming , Lee Ming-Chieh , Degefa Teshome , Getachew Dejene , Haileselassie Werissaw , Hawaria Dawit , Yewhalaw Delenasaw , Yan Guiyun
Published: September 2024

The invasion of Anopheles stephensi into Africa poses a potential threat to malaria control and elimination on the continent. However, it is not clear if the recent malaria resurgence in Ethiopia has linked to the expansion of An. stephensi. We obtained the clinical malaria case reports and malaria intervention data from the Ethiopian Ministry of Health (MoH) for the period 2001–2022. We analyzed clinical malaria hotspots and investigated the potential role of An. stephensi in the 2022 malaria outbreaks. Clinical malaria cases in Ethiopia decreased by 80%, from 5.2 million cases in 2004 to 1.0 million cases in 2018; however, cases increased steadily to 2.6 million confirmed cases in 2022. Plasmodium vivax cases and proportion have increased significantly in the past 5 years. Clinical malaria hotspots are concentrated along the western Ethiopian border areas and have grown significantly from 2017 to 2022. Major malaria outbreaks in 2022/2023 were detected in multiple sites across Ethiopia, and An. stephensi was the predominant vector in some of these sites, however, it was absence from many of the outbreak sites. The causes of recent upsurge in malaria in Ethiopia may be multi-factorial and it is a subject of further investigation.
Isolation and Selection of Protein-Rich Mutants of Chlorella vulgaris by Fluorescence-Activated Cell Sorting with Enhanced Biostimulant Activity to Germinate Garden Cress Seeds
Authors: Trovão Mafalda , Schüler Lisa , Pedroso Humberto , Reis Ana , Santo Gonçalo Espírito , Barros Ana , Correia Nádia , Ribeiro Joana , Bombo Gabriel , Gama Florinda , Viana Catarina , Costa Monya M. , Ferreira Sara , Cardoso Helena , Varela João , Silva Joana , Freitas Filomena , Pereira Hugo
Published: September 2024

Microalgae are a promising feedstock with proven biostimulant activity that is enhanced by their biochemical components (e.g., amino acids and phytohormones), which turns them into an appealing feedstock to reduce the use of fertilisers in agriculture and improve crop productivity and resilience. Thus, this work aimed to isolate protein-rich microalgal mutants with increased biostimulant activity. Random mutagenesis was performed with Chlorella vulgaris, and a selection of protein-rich mutants were sorted through fluorescence-activated cell sorting (FACS), resulting in the isolation of 17 protein-rich mutant strains with protein contents 19–34% higher than that of the wildtype (WT). Furthermore, mutant F4 displayed a 38%, 22% and 62% higher biomass productivity, growth rate and chlorophyll content, respectively. This mutant was then scaled up to a 7 L benchtop reactor to produce biomass and evaluate the biostimulant potential of this novel strain towards garden cress seeds. Compared to water (control), the germination index and the relative total growth increased by 7% and 19%, respectively, after the application of 0.1 g L−1 of this bioproduct, which highlights its biostimulant potential.
Identification of Sources of Resistance to Aphanomyces Root Rot in Pisum
Authors: Rodriguez-Mena Sara , Rubiales Diego , González Mario
Published: September 2024

Aphanomyces root rot (ARR), caused by Aphanomyces euteiches, is one of the most devastating diseases that affect the production of peas. Several control strategies such as crop rotation, biocontrol, and fungicides have been proposed, but none provides a complete solution. Therefore, the deployment of resistant cultivars is fundamental. ARR resistance breeding is hampered by the moderate levels of resistance identified so far. The available screening protocols require post-inoculation root assessment, which is destructive, time-consuming, and tedious. In an attempt to address these limitations, we developed a non-destructive screening protocol based on foliar symptoms and used it to identify new sources of resistance in a Pisum spp. germplasm collection. Accessions were root inoculated separately with two A. euteiches isolates, and leaf symptoms were assessed at 5, 10, 14, 17, and 20 days after inoculation (DAI). Although the majority of accessions exhibited high levels of susceptibility, thirty of them exhibited moderate resistance. These thirty accessions were selected for a second experiment, in which they were inoculated with both A. euteiches isolates at two inoculum doses. The objective of this second trial was to confirm the resistance of these accessions by evaluating root and biomass loss, as well as foliar symptoms, and to compare root and foliar evaluations. As a result, a high correlation (R2 = 0.75) between foliar and root evaluations was observed, validating the foliar evaluation method. Notably, accessions from P.s. subsp. humile exhibited the lowest symptomatology across all evaluation methods, representing valuable genetic resources for breeding programs aimed at developing pea varieties resistant to ARR.
Bacterial Volatile Organic Compounds as a Strategy to Increase Drought Tolerance in Maize (Zea mays L.): Influence on Plant Biochemistry
Authors: Lopes Tiago , Costa Pedro , Cardoso Paulo , Figueira Etelvina
Published: September 2024

Maize is highly susceptible to drought, which affects growth and yield. This study investigated how bacterial volatile organic compounds (BVOCs) affect maize drought tolerance. Drought reduced shoot size but increased root length, an adaptation for accessing deeper soil moisture. BVOCs from strain D12 significantly increased root length and shoot growth under drought conditions. Drought also altered root biochemistry, decreasing enzyme activity, and increased osmolyte levels. BVOCs from strains F11 and FS4-14 further increased osmolyte levels but did not protect membranes from oxidative damage, while BVOCs from strains D12 and D7 strains reduced osmolyte levels and cell damage. In shoots, drought increased the levels of osmolytes and oxidative stress markers. BVOCs from FS4-14 had minimal effects on shoot biochemistry. BVOCs from D12 and F11 partially restored metabolic activity but did not reduce cell damage. BVOCs from D7 reduced metabolic activity and cell damage. These results suggest that BVOCs can modulate the biochemical response of maize to drought, with some strains evidencing the potential to enhance drought tolerance.
Preliminary Exploration of Low Frequency Low-Pressure Capacitively Coupled Ar-O2 Plasma
Authors: Wali Niaz , Xiao Weiwen , Din Qayam Ud , Rehman Najeeb Ur , Wang Chiyu , Ma Jiatong , Zhong Wenjie , Yang Qiwei
Published: September 2024

Non-thermal plasma as an emergent technology has received considerable attention for its wide range of applications in agriculture, material synthesis, and the biomedical field due to its low cost and portability. It has promising antimicrobial properties, making it a powerful tool for bacterial decontamination. However, traditional techniques for producing non-thermal plasma frequently rely on radiofrequency (RF) devices, despite their effectiveness, are intricate and expensive. This study focuses on generating Ar-O2 capacitively coupled plasma under vacuum conditions, utilizing a low-frequency alternating current (AC) power supply, to evaluate the system’s antimicrobial efficacy. A single Langmuir probe diagnostic was used to assess the key plasma parameters such as electron density (ne), electron temperature (Te), and electron energy distribution function (EEDF). Experimental results showed that ne increases (7 × 1015 m−3 to 1.5 × 1016 m−3) with a rise in pressure and AC power. Similarly, the EEDF modified into a bi-Maxwellian distribution with an increase in AC power, showing a higher population of low-energy electrons at higher power. Finally, the generated plasma was tested for antimicrobial treatment of Xanthomonas campestris pv. Vesicatoria. It is noted that the plasma generated by the AC power supply, at a pressure of 0.5 mbar and power of 400 W for 180 s, has 75% killing efficiency. This promising result highlights the capability of the suggested approach, which may be a budget-friendly and effective technique for eliminating microbes with promising applications in agriculture, biomedicine, and food processing.
Computational Fluid Dynamics Modelling of a Laboratory Spray Dry Scrubber for SO2 Removal in Flue Gas Desulphurisation—Effect of Drying Models
Authors: Lerotholi Letsabisa , Everson Raymond C. , Hattingh Burgert B. , Koech Lawrence , Le Roux Ignus , Neomagus Hein W. J. P. , Rutto Hilary L.
Published: September 2024

Spray dry scrubbing is widely used for SO2 abatement, but high removal efficiencies are required for economical operation. Whereas SO2 removal dependence on the drying rate has been investigated, available modelling work has not addressed the impact of selected drying models on the removal efficiency; instead, a single drying model is often assumed. In the present work, computational fluid dynamics is used to numerically model the SO2 removal in a laboratory-scale spray dry scrubber. The Euler–Lagrangian framework is used to simulate the multiphase interaction and two drying models are used: the widely used classical D2-law model and the mechanistic model. In addressing shortcomings from previous works, this study also provides a comprehensive model development and robust model validation with quantifiable metrics for goodness-of-fit, including R2. Also presented are key parameters associated with SO2-removal efficiency, including the exit product moisture content and droplet dynamics. The mechanistic model gave a better representation of the SO2-removal efficiency. The latter was found to be dependent on the inlet temperature, the calcium-to-sulphur and liquid-to-gas (L/G) ratios, with a high L/G ratio having the most significant impact on the removal efficiency, although resulting in a higher product outlet moisture content.
Effect on Top-Coal Mass Failure under Load–Unload Induced by Shield Support
Authors: Ding Pengchu , Wang Hao , Zhao Jianjian , Yan Shiheng , Chang Liwu , Li Zhen , Zhou Changtai , Han Dong , Yang Jie
Published: September 2024

Prior to being released, the top coal of a fully mechanized caving face typically experiences the effects of mining-induced pressure and disturbances from the hydraulic support’s canopy. To investigate the promoting effect of canopy disturbances in the support-controlled area on the damage of top coal during fully mechanized top-coal caving mining, block instability theory and discrete block numerical simulation methods were employed. The instability modes during the initial support and cyclic disturbance stages were analyzed. The fracture evolution and displacement distribution curves were studied for coal bodies with strengths of 3.1 MPa, 15.0 MPa, and 29.5 MPa under the mining influence and support strengths of 0.5 MPa, 1.0 MPa, and 1.5 MPa during the initial support and cyclic disturbance processes. The results showed that during the initial support stage, the instability of the block-structured coal body exhibited four modes as follows: inter-block breakage, inter-block delamination, intra-block fracture expansion, and inter-block sliding. During the cyclic disturbance stage, the coal body instability showed three modes as follows: inter-block sliding, intra-block fracture expansion, and inter-block rotation. The disturbance from the support had little impact on the fragmentation of both low-strength and high-strength coal bodies. However, small support forces combined with multiple cycles of disturbance had an enhancing effect on the instability of medium-strength block-structured top coal.
Incineration Innovation: A Path to Efficient and Sustainable Municipal Solid Waste Management in Kuwait
Authors: AlMokmesh Saad F. , AlKhulaifi Khalid A. , AlMutairi Abdulrahman S. , Al-Ajmi Amani S.
Published: September 2024

Municipal solid waste management has become a critical global issue due to the rapid increase in waste generation driven by urbanization and population growth. This surge in waste poses significant environmental, social, and health challenges, exacerbated by inefficient recycling and waste-to-energy facilities. Effective waste management requires comprehensive strategies encompassing waste reduction, efficient collection, sorting systems, and advanced recycling and energy recovery technologies. This study highlights the potential of incineration as a waste-to-energy solution, specifically focusing on Kuwait. By analyzing various waste management technologies and their applicability, this study emphasizes the role of incineration in transforming municipal solid waste into electricity, thereby reducing landfill use and environmental impacts. The research includes a detailed review of the existing technologies, a case study on Kuwait’s waste management practices, and an evaluation of the economic and environmental benefits of implementing waste-to-energy incineration. The findings underscore the importance of tailored waste management solutions to address specific regional challenges, promote sustainability, and enhance public health and well-being.
Wandering Spleen Complicated by Thrombocytopenia, Acute Appendicitis, and Sepsis: A Case Report and Literature Review
Authors: Inggriani Sri , Sulay Callistus Bruce Henfry , Octavius Gilbert Sterling
Published: September 2024

Wandering spleen (WS) is a rare condition often linked with torsion or infarction, but its association with Bochdalek hernia, acute appendicitis, and thrombocytopenia is exceptionally rare. We present a case of a nine-year-old girl who was admitted with acute abdominal pain, later diagnosed with WS, Bochdalek hernia, and acute appendicitis. A literature search was performed on PubMed and Google Scholar on 30 May 2024 with keywords including “Wandering spleen” and (“Bochdalek Hernia” OR “Sepsis” OR “Acute Appendicitis” OR “Thrombocytopenia”). The management was complicated by severe thrombocytopenia and post-operative sepsis, with Klebsiella pneumoniae as the causative agent. Imaging revealed an abnormally located spleen and significant splenic enlargement over time. The patient’s condition was managed non-operatively concerning the WS, avoiding splenectomy due to the risks of post-splenectomy sepsis. Instead, laparotomy was performed to address the appendicitis and diaphragmatic hernia. The patient experienced post-operative complications, including a seizure and persistent fever, which resolved with appropriate antibiotic therapy. This case underscores the complexity of managing WS with concurrent severe conditions, highlighting the importance of individualised treatment strategies. It also emphasises the need for further studies to explore optimal treatment modalities for such rare and complex presentations. This case serves as an educational example in clinical settings, demonstrating the challenges and considerations when treating multiple rare pathologies simultaneously.
Ada-LT IP: Functional Discriminant Analysis of Feature Extraction for Adaptive Long-Term Wi-Fi Indoor Localization in Evolving Environments
Authors: Hailu Tesfay Gidey , Guo Xiansheng , Si Haonan , Li Lin , Zhang Yukun
Published: September 2024

Wi-Fi fingerprint-based indoor localization methods are effective in static environments but encounter challenges in dynamic, real-world scenarios due to evolving fingerprint patterns and feature spaces. This study investigates the temporal variations in signal strength over a 25-month period to enhance adaptive long-term Wi-Fi localization. Key aspects explored include the significance of signal features, the effects of sampling fluctuations, and overall accuracy measured by mean absolute error. Techniques such as mean-based feature selection, principal component analysis (PCA), and functional discriminant analysis (FDA) were employed to analyze signal features. The proposed algorithm, Ada-LT IP, which incorporates data reduction and transfer learning, shows improved accuracy compared to state-of-the-art methods evaluated in the study. Additionally, the study addresses multicollinearity through PCA and covariance analysis, revealing a reduction in computational complexity and enhanced accuracy for the proposed method, thereby providing valuable insights for improving adaptive long-term Wi-Fi indoor localization systems.
Control Circuits for Potentiostatic/Galvanostatic Polarization and Simultaneous Chemical Sensing by a Light-Addressable Potentiometric Sensor
Authors: Yoshinobu Tatsuo , Ikeda Rintaro , Miyamoto Ko-ichiro
Published: September 2024

A light-addressable potentiometric sensor (LAPS) is a semiconductor-based sensor platform for sensing and imaging of various chemical species. Being a potentiometric sensor, no faradaic current flows through its sensing surface, and no electrochemical reaction takes place in the course of LAPS measurement. In this study, a four-electrode system is proposed, in which a LAPS is combined with the conventional three-electrode electrochemical system. A LAPS is included as the fourth electrode for potentiometric sensing and imaging of the target analyte in the course of an electrochemical reaction taking place on the surface of the working electrode. The integrated system will be useful for analyzing dynamic processes, where both the electrochemical process on the electrode surface and the ion distribution in the solution need to be simultaneously investigated. Different grounding modes of control circuits that can simultaneously conduct potentiostatic/galvanostatic polarization and LAPS measurement are designed, and their functionalities are tested. The interference between polarization and LAPS measurement will also be discussed.