bluuebunny/crossref_metadata_2025 huggingface dataset:
Interferencia de heparina en la determinación de proteínas totales por el método de Bradford
Authors: Cabanillas Laura M. , Da Silva María A. , Marinsaldi Anabella , Bernardi María E.
Published: January 2024

La determinación de proteínas totales es una técnica de rutina utilizada en la industria biofarmacéutica durante los procesos de purificación de derivados plasmáticos y/o producción de proteínas recombinantes; y la elección del método analítico es un paso crítico para la obtención de resultados confiables. En este trabajo se demostró la interferencia de heparina (Hep), un compuesto comúnmente utilizado como excipiente en la formulación de Concentrados de Factores de la Coagulación (CFC), en la determinación de proteínas por el método de Bradford, cuya interferencia no está descripta como tal en los insertos de los kits comerciales de Bradford ni en la Farmacopea Europea. Esta interferencia fue demostrada tanto en soluciones de Albúmina Sérica Bovina (BSA) fortificadas con Hep, como en muestras de CFC. Para una solución de BSA 40 mg %, la concentración límite de Hep que no interfiere en la determinación de proteínas totales fue de 1,6 UI/ml. Cabe destacar que, si se trabaja a un nivel menor de concentración de proteínas, una proteína diferente o mezclas complejas de proteínas, se debe evaluar el grado de interferencia para cada caso particular, pudiéndose utilizar los valores obtenidos en este trabajo como referencia. Alternativamente, se evaluó la neutralización de la Hep con Polybrene (Poly), previo a la determinación de proteínas por Bradford. En el caso de soluciones de BSA, el efecto interferente se revirtió logrando una correcta cuantificación de proteínas, mientras que en las muestras de CFC, la neutralización con Poly no fue efectiva. Por lo tanto, para considerar el uso de Poly, previo a la aplicación del método de Bradford, debe evaluarse cada caso particular según las características de la muestra proteica en estudio. Si esto no es posible, se recomienda la utilización de un método alternativo, por ejemplo, el colorimétrico que utiliza ácido bicinconínico.
Contenido de yodo de la sal alimentaria en puestos de venta del Gran San Salvador de Jujuy, Argentina (Mayo 2022)
Authors: López Linares Sonia
Published: January 2024

El objetivo de este trabajo fue determinar la calidad de yodación de la sal alimentaria a nivel de puestos de venta en los barrios del Gran San Salvador de Jujuy (GSSJ). Para esto se llevó a cabo un estudio descriptivo y transversal. Se adquirió a nivel comercial un envase de sal en cada una de las 47 urbanizaciones del GSSJ, durante el mes de mayo del 2022. El yodo en sal se determinó por titulación con tiosulfato de sodio, con valor referencial establecido por la Ley 17.259/69 (toda sal debe estar yodada en un rango de una parte de yodo en 30.000 de sal ± 25 % = 24,7–41,2 ppm). Los resultados mostraron que el nivel de yodo en las sales fue de 24,5 ± 9,4 ppm (promedio ± desvío estándar), 26,1 ppm de mediana, 0 a 42,5 ppm (rango) y IC 95 % de 21,8–27,2 ppm. Solo el 53,2 % de las muestras estaban correctamente yodadas, el 40,4 % contenían yodo, pero de manera insuficiente, el 4,3 % estaban sin yodar y el 2,1 % en exceso marginal, con similar distribución en todas las categorías del NBI/urbanización (p=NS). Solo el 45,0 % de las sales envasadas regionales cumplían con el nivel de yodo adecuado, diferencia altamente significativa respecto a las no regionales (p=0,0077). En conclusión, en el expendio del mercado minorista del GSSJ casi la mitad de los productos necesitan optimizar el nivel de yodación, por lo que se hace necesario aunar los esfuerzos de todos los actores participantes ante la imperiosa necesidad de cumplir no solo con las normativas y parámetros referenciales, sino fundamentalmente con el aporte del nutriente clave para cubrir los requerimientos diarios de la población.
Assessment of Tax Enforcement in Selected Small and Medium Enterprises (SMEs) in Kigali City, Rwanda
Authors:   University of Lay Adventists of Kigali, NTAHONDEREYE Aminadab
Published: January 2024

The purpose of the study was to assess the level of tax enforcement as it is exerted by Rwanda Revenue Authority (RRA) among SMEs in Kigali city. The indicators under consideration are Tax system, Training on tax, Tax Audit, Tax rewards and Tax penalties. Administered questionnaire was used to collect data. Finding showed that generally, tax system was appreciated at a moderate level (Mean=2.67). The average level of RRA trainings is low (Mean=2.31). The General level of tax audit practice is high level (Mean=3.72). Generally, the level of Tax Rewarding is Moderate (Mean=3.37). Generally, the level of appreciation on Tax Penalties was Moderate (Mean=2.53). As recommendations, RRA should change its tax system. This is because level of appreciation of the taxpayer to the system is moderate with a mean of 2.67. The are to improve include helping taxpayers to pay only when they have made profits. Facilitating the taxpayer to increase the capacity to pay their tax liabilities, making tax law friendly user by SMEs taxpayers. RRA should increase training for SMEs taxpayers. This is because the level of this indicator is low. The low level has been found in all areas of training assessed. RRA should increase the rewords to the best taxpayers so that this practice is recognized by all taxpayers. It should also avail them the standards of selection and to the public. This will increase trust and competition in complying to the tax laws.RRA should modify the penalties given to taxpayers who have broken the tax laws. This is because the taxpayers said that the tax penalties discourage many SMEs. The tax penalties should be reduced and increase other methods of enforcement such as education, rewords and audit. Taxpayers should inform themselves from different sources about the tax system as applicable in Rwanda. This will help them to comply with the law and avoid penalties
QoS for Device-to-Device communication in 4G LTE-A network
Authors:   University of Lay Adventists of Kigali, HAGENIMANA Jean de Dieu
Published: January 2024

Device to device (D2D) communication is a technology model that enables mobile devices to communicate directly with each other without the intervention of a base station or enabled Node B (eNB). It helps the reuse of LTE-Advanced radio frequency resources of the network. With this paper, we simulated two metrics in delay and throughput. According to simulation results, D2D mode is the best option for communication when two mobile devices are close to one another but far from the eNB.
Assessment of Tax Compliance in Selected Small and Medium Enterprises (SMEs) in Kigali City, Rwanda
Authors:   University of Lay Adventists of Kigali, NTAKIRUTIMANA Elison , NTAHONDEREYE Aminadab
Published: January 2024

The objective of the study was to assess the level of tax compliance among SMEs in Kigali city in terms of Taxpayers ’registration, keeping books and records, Fulfilling tax obligation on time and attitude to tax evasion. Taxpayers ’registration is at a moderate level (Mean=2.31). Keeping books and records is done at a moderate level (Mean= 2.91). In general, the level of fulfilling tax obligations on time is moderate (Mean= 3.04). In general, negative attitude to tax evasion is at a high level (Mean= 3.42). These findings were in line with the study conducted by Isbell (2017) where he showed that on average across 36 countries, 63% of Africans say taxes are necessary for development. Large majorities say paying taxes is a civic duty (72%) and not paying taxes is wrong (87%) though many describe non-compliance as wrong but understandable. About recommendations, RRA must increase the effort in the enforcement, like organizing more training and mobilization to SMEs taxpayers because many of them do not comply because of ignorance. Taxpayers should participate in training organized by RRA. They should inform themselves from different sources about the tax law as applicable in Rwanda. This will help them to comply and hence avoid penalties. They should understand that the purpose and benefits of paying taxes.
Effect of Loan Repayment on Financial Performance of SACCOs in Rwanda A Case of Umwalimu SACCO Head Office
Authors:   University of Lay Adventists of Kigali, NSHIMYIMANA Augustin , NKURUNZIZA Prosper
Published: January 2024

Consequently, Poor loan repayments have a negative effect on an institution's capital, earnings, and ability to achieve its goals, and they may even cause a financial institution to fail. For instance, poor loan repayment management results in losses and expensive delinquent management expenses. Such expenses have a negative impact on the income earned and, generally, the operations of the lending institution; as a result, the institution becomes financially unsustainable. The main objective of this study was to analyze the effect of loan repayment on financial performance of SACCOs in Rwanda and specific was to assess the effect of loan period on the financial performance of UMWALIMU SACCO for the period under study, to analyses the effect of loan follow-up policy on the financial performance of UMWALIMU SACCO for the period under study and to examine the effect of loan recovery policy on the financial performance of UMWALIMU SACCO for the period under study. Descriptive research design was used for this study, while Regression analysis was also employed. It provides empirical evidence suggesting two or more variables are or are not related and the researcher use a sample of 33 employees of UMWALIMU SACCO, therefore: financial performance of UMWALIMU SACCO main Branch = 16.631+ .361 loan period + .712 follow up policy +.584 loan recovery policy. At 5% level of significance all three variables which are Loan Period, Loan Follow-up Policy, and Loan Recovery Policy were positively and statistically significance since their p-values was below the acceptable threshold of 0.05. From the research findings, positive effect was found on three the variable i.e. is Loan Period, Loan Follow-up Policy, and Loan Recovery Policy with regression coefficients of 0.361, 0.712 and 0.584 respectively. These findings suggest that a unit increase in loan period, taking all the other variables constant at zero would result to a 0.361 increase in financial performance in UMWALIMU SACCO. Similarly, a unit increase in loan follow up policy would result to 0.712 increases in financial performance of UMWALIMU SACCO. A unit increase in loan recovery policy would result to 0.584 increases in financial performance of UMWALIMU SACCO main branch. Therefore, this study recommends that credit officers and credit committees should go beyond customer character before approving loans but assess other factors such as job security and the amount borrowed by the prospect.
Desenvolvimento de uma versao online e gratuita do Female Sexual Function Index - FSFI
Authors: Paula Reis Pessoa Ana
Published: January 2024

Background: Female sexual dysfunction (FSD) is very common in our society and is therefore a major public health problem. Aims: To develop a free online version of the Female Sexual Function Index - FSFI. Method: This is an experimental study that aimed to develop an online, electronic version of the FSFI questionnaire for data collection from epidemiological studies that encompass female sexual function. Results: The Google Forms platform was the one that best fit the study proposal. Google Forms is a free, online form creation site from Google that allows you to view and analyze your data, and for that reason was the tool chosen to transcribe the FSFI questionnaire. Conclusion: The present study developed a free online version of the Brazilian version of the FSFI questionnaire, the most used worldwide in DSF research, in order to allow free access of researchers who need to use this questionnaire, regardless of where the study is developed, universalizing access and optimizing data collections of this type.
Fisioterapia sobre a disfuncao eretil de pacientes não prostatectomizados
Authors: F Sutter Latorre Gustavo
Published: January 2024

Background: Erectile dysfunction (ED) is defined as the persistent inability to obtain or maintain a penile erection sufficient for satisfactory sexual performance, which may indicate the existence of underlying diseases, especially those related to the cardiovascular system. From the knowledge of the etiology, physiotherapy can outline goals and procedures for the treatment of this patient. Aims: Raise the range of resources available to physical therapy treatment. Method: Integrative review, through electronic searches carried out in the Pubmed, LILACS, PEDro and Scielo databases, using the keywords erectile dysfunction and physiotherapy, as well as their variants in English, including studies that mentioned some type of physiotherapy for these patients, and excluding duplicated studies in the databases, studies where pharmacotherapy was adjuvant to physiotherapy and those that dealt with the treatment of prostatectomy patients. Results: 87 articles were found, of these 11 selected according to the inclusion criteria. Conclusion: little or no scientific attention has been given to men with ED who have not undergone prostatectomy, although these are potential patients for complete reversal of the condition by pelvic physical therapy. New studies are urgently needed.
Eletroestimulacao especial para incremento da funcao sexual feminina aumenta lubrificação e sensibilidade vaginal
Authors: F Sutter Latorre Gustavo
Published: January 2024

Background: Female sexual dysfunction afflicts more than half of Brazilian women, with prevalente complaints of low intravaginal sensitivity and lubrication. Although electrical stimulation has clinical effects on mucous membranes, there are no studies in this field. Aims: To test a specifically designed electrical stimulation to improve female sexual function, by improving lubrication and intravaginal sensitivity Method: Clinical trial where 38 women with various sexual dysfunctions were treated for 4 weeks, evaluating sexual function and qualitative perceptions. Results: There was an improvement in the mean FSFI score before (25.6±4) and after (29.4±4), surpassing the sexual dysfunction range. Volunteers described objective increase in vaginal sensitivity and lubrication. Conclusion: Sexual function is far more than the absence of dysfunction, and some non-excitomotor electrostimulation can be a useful tool to increase intravaginal lubrication and sensitivity in young and middle-aged women. Further studies are needed for other populations.
Incidencia de disfuncao sexual em ate dois anos pos parto - estudo transversal
Authors: Antoneli Rech Jaqueline
Published: January 2024

Background: Several factors are responsible for making the pelvic floor muscles weak, among them is pregnancy, a period in which several changes occur in the woman's body. Female sexual dysfunctions are disorders that can appear in some of the phases of a woman's sexual response cycle, making it difficult to achieve pleasure during intercourse. Aims: Investigate the incidence of sexual dysfunction in women who had a single birth in less than two years. Method: This is a quantitative, descriptive exploratory study, data collection was performed through questionnaires: sample characterization, FSFI (Female Sexual Function Index) and QS-F (sexual quotient-female version). Results: 40 women participated in the study with a mean age of 26.2 years in which 80% of the sample does not have sexual dysfunction. Conclusion: sexual dysfunction was uncommon in the two-year postpartum period. However, when we consider the questionnaires developed by the researchers, there is a higher rate of sexual dysfunction.
Flatos vaginais durante o sexo sao fisiologicos e relacionados ao prazer e posturas
Authors: F Sutter Latorre Gustavo
Published: January 2024

Background: Vaginal flatus is a prevalent symptom, described by the absolute majority of women and correlated in the literature with pelvic floor dysfunction (PAD). But do the absolute majority of women, then, have enough PAD to justify these flatus? Aims: To describe the prevalence of vaginal flatus in the general population, test the correlation between vaginal flatus and sexual pleasure and propose a new functional classification for the phenomenon. Method: Descriptive exploratory study. Volunteers summoned on social networks answered sociodemographic, sexual, obstetric and vaginal flatus questions. Results: Of the 557 volunteers, 97.3% had vaginal flatus, related to positions of greater sexual pleasure, and antigravity sexual postures. The postures “amazon” and “missionary” were the most pleasurable, while “four supports” was responsible for the majority (78.3%) of flatus. The moment of undoing intercourse and changing postures after sex caused vaginal air to escape for 95.9% of women. Conclusion: Vaginal flatus during or as a result of sex is normal and physiological, occurring due to antigravity postures for 90% of women and for sexual pleasure itself for 30% of women. Vaginal flatus outside the sexual context indicate pelvic floor dysfunctions, which must be evaluated and treated. Healthcare professionals who venture out as digital influencers must act responsibly in addressing these differences.
Crencas parentais nas praticas maternas de gestantes em Parnaiba PI
Authors: Vitória Gomes de Sousa Mayara
Published: January 2024

Background: Pregnancy is a period of many physiological adaptations, as well as the structuring of beliefs that form the basis of maternal care, given that there are specificities that depend on the culture and family context, impacting the relationship that caregivers establish with the child and the environment in which the family nucleus is inserted. Aims: The present study aimed to evaluate the beliefs that support maternal care, as well as the importance given to each attitude and/or thought related to daily practices in the care of children that can influence child development. Method: The sample was formed by 100 pregnant women, residents in the city of Parnaíba-PI, who answered, virtually, the questionnaire of maternal beliefs and practices about child care. Results: Data analysis showed that participating mothers share parental beliefs about childcare practices, even with different sociodemographic characteristics. In addition, the results obtained showed that the “Stimulation” dimension was valued first, followed by the “Presentation”, “Bonding” and “Discipline” dimensions. Conclusion: The results found in the present research provide an understanding of the conceptions of the world that families have, and can support professional actions aimed at both children and parents.
Prevencao de laceracao perineal no parto vaginal: revisao
Authors: Rosa Vargas Wiethan Jessica
Published: January 2024

Background: The vaginal tear in vaginal delivery is a recurring and worrying factor for the health of parturients, so, this necessary to know the most effective prevention strategies, so that perineal traumas are minimized. Aims: to investigate in the literature what evidence is available on strategies to prevent the occurrence of perineum tears. Method: Narrative review of the literature was made out from March to April 2020, in the LILACS, MEDLINE and SCIELO databases using the keywords “vaginal delivery”, “perineum” and “episiotomy”. Results: Initially, 86 articles were found, 54 were excluded because they did not fit the theme, 32 remained, and after the inclusion and exclusion criteria were applied, leaving 7 productions. Conclusion: it was possible to observe that the most used strategies to obtain perineal integrity after vaginal deliveries are: peri-neal massage and Epi-no during pregnancy, favorable position during childbirth, manual protection of the perineum, hands on and hands off during delivery.
Liberacao miofascial pelvica profunda (Manobra do Ligamento Largo) associada ou nao ao LPF
Authors: F Sutter Latorre Gustavo
Published: January 2024

Background: Among genitopelvic pain is the pain deep into the vagina and deeper than the uterine cervix, different from pain related to penetration or superficial connective pain. Aims: To test the view of abdominal and pelvic fascia, muscles and viscera by ultrasound (USG), as well as the view of two myofascial techniques, one intravaginal and the other external, and diaphragmatic aspiration of LPF, comparing the effectiveness of each technique alone or in combination, regarding the mobilization of visceral and parietal fasciae. Method: Exploratory experimental study guided by USG. Results: Muscles, fasciae and organs were well seeing by USG, as well as the fascial movements caused by each technique. LPF mobilized visceral fascia better, but manual techniques mobilized parietal fascia better. The Broad Ligament Maneuver mobilized both fasciae. The combination of manual techniques with LPF was superior in releasing parietal and visceral fasciae. Conclusion: Fascial mobilization can be effectively visualized by USG. The combination of manual myofascial release techniques with LPF is more effective in mobilizing all fasciae and should be the first choice.
Perfil de usuarias das UBS do Distrito Leste de Foz do Iguacu com IU: Pesquisa de Campo
Authors: Pereira Armstrong Fabiana
Published: January 2024

Background: Panorama: Women with Urinary Incontinence treatment use them as Basic Units (BHU) due to the lack of perspective on the level of care. Objective: To identify the profile of women with UI in the Basic Health Units of the East District of Foz do Iguaçu and the impact of UI on these women's quality of life. Method: Data collection with women waiting to attend the Basic Health Units of the District of Foz do Iguaçu, applying a question with objective questions about age, weight, type of delivery, type of UI and whether they are aware of physical therapy to solve this problem. problem. Results: Interviewed 82 women aged 19 to 80 years. As for UI, 34.1 reported some type: Stress UI 17.1% and Urgent UI 31.7%, about physical therapy, 61.0% were unaware. Conclusion: Many users of Basic Health Units are aware of UI treatment units, thus being necessary for the creation of treatment and promotion programs on UI to solve this problem as well as the benefits of physical therapy.
Fisioterapia pelvica no tratamento da incontinencia urinária de mulheres manauaras
Authors: Natália Cardoso Maria
Published: January 2024

Background: Despite the gold standard in the treatment of urinary incontinence (UI) since 2005, specific studies on the population of Manaus, Brazil, are scarce. Aims: To evaluate the effectiveness of Pelvic Physiotherapy in women with UI. Method: Quasiexperimental study in women registered at the Open University Foundation for the Third Age, with complaints of UI, excluding those who missed three or more appointments. Results: A total of 24 women participated in the study with a mean age of 60.41±9.15 years. The most reported type of urinary incontinence was Stress. There was an improvement in symptoms in all participants who lost urine in a stream. More than half (66.66%) reported improvement in symptoms at reassessment. Conclusion: Pelvic Physiotherapy is efficient in the treatment of urinary incontinence in women along with the improvement of symptoms, leading to an improvement in quality of life.
Prevalencia de incontinencia urinaria em mulheres saudaveis em Guarapuava PR
Authors: Monteiro Pasqualin Caroline
Published: January 2024

Background: The pelvic floor has the function of supporting the pelvic viscera, sexual function, urinary and fecal continence, during pregnancy and childbirth, it allows the support and passage of the fetus. The failure of this musculature can cause urinary incontinence, which is defined as any involuntary loss of urine, which may generate social or hygienic discomfort for the patient. Aims: To investigate the prevalence of urinary incontinence in women over 18 years old, analysis of the degree of intimate selfknowledge and the interference of UI in quality of life. Method: Cross-sectional study of an investigative nature. A sociodemographic and PRAFAB questionnaire was used by Google Forms, through a link posted on social networks. Results: 62 women with an average age of 24.8 years participated, 77.4% had some level of urinary loss. Conclusion: Urinary incontinence was prevalent in young women, with no relationship between the severity of UI and the mode of delivery, but there are several factors that may be linked to this dysfunction.
Conhecimentos gerais sobre a saude do assoalho pelvico em mulheres de Guarapuava PR
Authors: Gabriela Cenci Ana
Published: January 2024

Background: The pelvic floor is composed of muscles, ligaments, fascia; tissues responsible for supporting the pelvic viscera, controlling the urethra, anal sphincter and sexual function, also acting in childbirth. It is essential that the woman has selfknowledge about her body so as not to develop dysfunctions. Aims: To analyze the level of self-knowledge that the female population has about function, location and dysfunctions related to the pelvic floor. Method: Quantitative and qualitative crosssectional study, carried out through a questionnaire applied during an informative lecture. Results: Of the 50 participants, most of the questions showed good knowledge, but the level of education was related to the level of knowledge. Conclusion: Women in the region have good knowledge of pelvic floor health, but some had difficulty identifying and differentiating between normal and pathological processes in the body. There is a need for investment in educational activities, especially for the less favored educational strata.
Fisioterapia pevica na reabilitacao das disfuncoes do assoalho pelvico de mulheres submetidas a redesignacao sexual: revisao
Authors: Machado Canterle Eduarda
Published: January 2024

Background: Transsexuality is a gender incongruity, that is, there is a divergence between the gender at birth and the individual's identification, where in some cases surgeries may be required in order to provide sexual adequacy for which surgery can help. However, it is pointed out that such surgical procedures may lead to postoperative complications, negatively impacting the pelvic floor. Aims: to review the effects of pelvic physiotherapy on pelvic floor dysfunctions in the sexual reassignment postoperative period. Method: Integrative review of PubMed databases EMBASE, LILACS, Scielo, Cochrane Library and PEDro using the terms “sexual reassignment surgery”, “physiotherapy”, “transgender woman”, “vaginoplasty”. Results: Found 50 articles, 3 included. Pelvic physiotherapy has a significant impact on conditions such as stenosis, muscle weakness, intravaginal fibrosis, AP incoordination, pain and functionality. Conclusion: despite the scarcity of studies, pelvic floor muscle training showed benefits in the function of patients undergoing vaginoplasty. Patients treated from the preoperative period have a tendency to obtain more effective results.
Fortalecimento do assoalho pelvico nas Disfuncoes Sexuais Femininas: revisao
Authors: Jeanne Rocha Keyla
Published: January 2024

Background: Pelvic floor muscle strength and sexual function in women are important variables in Pelvic Physiotherapy. Aims: To identify the main contributions of Physiotherapy to strengthening the pelvic floor muscles in women. Method: Bibliographical review of books, scientific articles, theses and dissertations published between 2007 and 2023. Results: The pelvic floor is a muscular and ligamentous structure that supports the organs in the lower abdomen. Its weakness can cause a variety of problems, including UI, prolapse, pain, and sexual dysfunction. Pelvic Physiotherapy works to rehabilitate these problems. Its exercises aim to strengthen and relax the muscles, normalizing tone and desensitizing the genital region. In the long term, they restore function, helping women to have a more satisfactory sex life. Conclusion: Muscle strengthening, proprioception and body awareness exercises can help improve the quality of sexual life, making it more satisfying.
El encaje jurídico-constitucional de la personalidad jurídica del Mar Menor y su cuenca
Authors: García Guijarro Pedro
Published: January 2024

La Ley 19/2022, para el reconocimiento de personalidad jurídica a la laguna del Mar Menor y su cuenca, es la norma más innovadora en materia de protección medioambiental incorporada en nuestro ordenamiento hasta el momento. Con ella, se pretende avanzar en las garantías tuitivas del ecosistema del Mar Menor, por medio de nuevos instrumentos normativos informados por criterios ecocéntricos. En consecuencia, se plantea una transformación del paradigma jurídico-relacional con la naturaleza, superándose las perspectivas antropocéntricas y desarrollando los principios de la justicia ecológica ecocéntrica. Ello puede implicar la confrontación entre la nueva ley y el ordenamiento jurídico previo, así como con la jurisprudencia sostenida por el Tribunal Constitucional y el Tribunal Europeo de Derechos Humanos. En este artículo estudiamos el marco normativo y jurisprudencial en que se inserta la Ley 19/2022 para, a continuación, acometer un análisis crítico de la norma, planteando las numerosas problemáticas jurídicas identificadas.
Jorge Castellanos Claramunt: La participación ciudadana en el ámbito local. La integración democrática de lo local y de lo global en la era digital
Authors: Imormino De Haro María Guadalupe
Published: January 2024

Recensión del libro de Jorge Castellanos Claramunt: La participación ciudadana en el ámbito local. La integración democrática de lo local y de lo global en la era digital, Valencia, Corts Valencianes, 2020.
An empirical formula of nuclear β-decay half-lives
Authors: Xia Jin-Ge , Li Wei-Feng , Fang Ji-Yu , Niu Zhong-Ming ,   School of Physics and Optoelectronic Engineering, Anhui University, Hefei 230601, China,   School of Mechanics and photoelectric Physics, Anhui University of Science & Technology, Huainan 232001, China
Published: January 2024

Nuclear β-decay half-lives play an important role not only in nuclear physics, but also in astrophysics. The β-decay half-lives of many nuclei involved in the astrophysical rapid neutron-capture (r -process) still cannot be measured experimentally, so the theoretical predictions of nuclear β-decay half-lives are inevitable for r-process studies. Theoretical models for studying the nuclear β-decay half-lives include the empirical formula, the gross theory, the quasiparticle random phase approximation (QRPA), and the shell model. Compared with other theoretical models of β-decay half-lives, the empirical formula has high computational efficiency, and its prediction accuracy can be improved by introducing more and more physical information. In this work, an empirical formula without free parameters is proposed to calculate the nuclear β-decay half-lives based on the Fermi theory of β decay. By including the pairing effect, the shell effect, and the isospin dependence, the newly proposed empirical formula significantly improves the accuracy of predicting the nuclear β-decay half-life. For the nuclei with half-lives less than 1 second, the root-mean-square deviation of the common logarithms of the nuclear β-decay half-life predicted by the new empirical formula from the experimental data decreases to 0.220, which is improved by about 54% compared with that by the empirical formula without free parameters, even better than those by other existing empirical formulas and microscopic QRPA approaches. In the unknown region, the nuclear β-decay half-lives predicted by the new empirical formula are generally shorter than those predicted by the microscopic models in the light nuclear region, while those predicted by the new empirical formula in the heavy nuclear region are generally in agreement with those predicted by the microscopic models. The half-lives of neutron-rich nuclei on the nuclear chart are then predicted by the new empirical formula, providing nuclear β-decay half-life inputs for the r-process simulations.
Engineering paralog-specific PSD-95 recombinant binders as minimally interfering multimodal probes for advanced imaging techniques
Authors: Rimbault Charlotte , Breillat Christelle , Compans Benjamin , Toulmé Estelle , Vicente Filipe Nunes , Fernandez-Monreal Monica , Mascalchi Patrice , Genuer Camille , Puente-Muñoz Virginia , Gauthereau Isabel , Hosy Eric , Claverol Stéphane , Giannone Gregory , Chamma Ingrid , Mackereth Cameron D , Poujol Christel , Choquet Daniel , Sainlos Matthieu
Published: January 2024

Despite the constant advances in fluorescence imaging techniques, monitoring endogenous proteins still constitutes a major challenge in particular when considering dynamics studies or super-resolution imaging. We have recently evolved specific protein-based binders for PSD-95, the main postsynaptic scaffold proteins at excitatory synapses. Since the synthetic recombinant binders recognize epitopes not directly involved in the target protein activity, we consider them here as tools to develop endogenous PSD-95 imaging probes. After confirming their lack of impact on PSD-95 function, we validated their use as intrabody fluorescent probes. We further engineered the probes and demonstrated their usefulness in different super-resolution imaging modalities (STED, PALM, and DNA-PAINT) in both live and fixed neurons. Finally, we exploited the binders to enrich at the synapse genetically encoded calcium reporters. Overall, we demonstrate that these evolved binders constitute a robust and efficient platform to selectively target and monitor endogenous PSD-95 using various fluorescence imaging techniques.
Gut microbiota-derived gamma-aminobutyric acid from metformin treatment reduces hepatic ischemia/reperfusion injury through inhibiting ferroptosis
Authors: Wang Fangyan , Liu Xiujie , Huang Furong , Zhou Yan , Wang Xinyu , Song Zhengyang , Wang Sisi , Wang Xiaoting , Shi Dibang , Ruan Gaoyi , Ji Xiawei , Zhang Eryao , Tan Zenglin , Ye Yuqing , Wang Chuang , Zhu Jesse , Wang Wantie
Published: January 2024

Hepatic ischemia/reperfusion injury (HIRI) is a common and inevitable factor leading to poor prognosis in various liver diseases, making the outcomes of current treatments in clinic unsatisfactory. Metformin has been demonstrated to be beneficial to alleviate HIRI in recent studies, however, the underpinning mechanism remains unclear. In this study, we found metformin mitigates HIRI-induced ferroptosis through reshaped gut microbiota in mice, which was confirmed by the results of fecal microbiota transplantation (FMT) treatment but showed the elimination of the beneficial effects when gut bacteria were depleted using antibiotics. Detailedly, through 16S rRNA and metagenomic sequencing, we identified that the metformin-reshaped microbiota was characterized by the increase of gamma-aminobutyric acid (GABA) producing bacteria. This increase was further confirmed by the elevation of GABA synthesis key enzymes, glutamic acid decarboxylase (GAD) and putrescine aminotransferase (PAT), in gut microbes of metformin-treated mice and healthy volunteers. Furthermore, the benefit of GABA against HIRI-induced ferroptosis was demonstrated in GABA-treated mice. Collectively, our data indicate that metformin can mitigate HIRI-induced ferroptosis by reshaped gut microbiota, with GABA identified as a key metabolite.
Asymmetric framework motion of TCRαβ controls load-dependent peptide discrimination
Authors: Chang-Gonzalez Ana C , Mallis Robert J , Lang Matthew J , Reinherz Ellis L , Hwang Wonmuk
Published: January 2024

Mechanical force is critical for the interaction between an αβ T cell receptor (TCR) and a peptide-bound major histocompatibility complex (pMHC) molecule to initiate productive T-cell activation. However, the underlying mechanism remains unclear. We use all-atom molecular dynamics simulations to examine the A6 TCR bound to HLA-A*02:01 presenting agonist or antagonist peptides under different extensions to simulate the effects of applied load on the complex, elucidating their divergent biological responses. We found that TCR α and β chains move asymmetrically, which impacts the interface with pMHC, in particular the peptide-sensing CDR3 loops. For the wild-type agonist, the complex stabilizes in a load-dependent manner while antagonists destabilize it. Simulations of the Cβ FG-loop deletion, which reduces the catch bond response, and simulations with in silico mutant peptides further support the observed behaviors. The present results highlight the combined role of interdomain motion, fluctuating forces, and interfacial contacts in determining the mechanical response and fine peptide discrimination by a TCR, thereby resolving the conundrum of nearly identical crystal structures of TCRαβ-pMHC agonist and antagonist complexes.
Commissureless acts as a substrate adapter in a conserved Nedd4 E3 ubiquitin ligase pathway to promote axon growth across the midline
Authors: Sullivan Kelly G , Bashaw Greg J
Published: January 2024

In both vertebrates and invertebrates, commissural neurons prevent premature responsiveness to the midline repellant Slit by downregulating surface levels of its receptor Roundabout1 (Robo1). In
Drosophila
, Commissureless (Comm) plays a critical role in this process; however, there is conflicting data on the underlying molecular mechanism. Here, we demonstrate that the conserved PY motifs in the cytoplasmic domain of Comm are required allow the ubiquitination and lysosomal degradation of Robo1. Disruption of these motifs prevents Comm from localizing to Lamp1 positive late endosomes and to promote axon growth across the midline
in vivo
. In addition, we conclusively demonstrate a role for Nedd4 in midline crossing. Genetic analysis shows that
nedd4
mutations result in midline crossing defects in the Drosophila embryonic nerve cord, which can be rescued by introduction of exogenous Nedd4. Biochemical evidence shows that Nedd4 incorporates into a three-member complex with Comm and Robo1 in a PY motif-dependent manner. Finally, we present genetic evidence that Nedd4 acts with Comm in the embryonic nerve cord to downregulate Robo1 levels. Taken together, these findings demonstrate that Comm promotes midline crossing in the nerve cord by facilitating Robo1 ubiquitination by Nedd4, ultimately leading to its degradation.
Commissureless acts as a substrate adapter in a conserved Nedd4 E3 ubiquitin ligase pathway to promote axon growth across the midline
Authors: Sullivan Kelly G. , Bashaw Greg J.
Published: January 2024

In both vertebrates and invertebrates, commissural neurons prevent premature responsiveness to the midline repellant Slit by downregulating surface levels of its receptor Roundabout1 (Robo1). In
Drosophila
, Commissureless (Comm) plays a critical role in this process; however, there is conflicting data on the underlying molecular mechanism. Here, we demonstrate that the conserved PY motifs in the cytoplasmic domain of Comm are required allow the ubiquitination and lysosomal degradation of Robo1. Disruption of these motifs prevents Comm from localizing to Lamp1 positive late endosomes and to promote axon growth across the midline
in vivo
. In addition, we conclusively demonstrate a role for Nedd4 in midline crossing. Genetic analysis shows that
nedd4
mutations result in midline crossing defects in the Drosophila embryonic nerve cord, which can be rescued by introduction of exogenous Nedd4. Biochemical evidence shows that Nedd4 incorporates into a three-member complex with Comm and Robo in a PY motif-dependent manner. Finally, we present genetic evidence that Nedd4 acts with Comm in the embryonic nerve cord to downregulate Robo1 levels. Taken together, these findings demonstrate that Comm promotes midline crossing in the nerve cord by facilitating Robo ubiquitination by Nedd4, ultimately leading to its degradation.
Exploring accidental virulence
Authors: Smith Daniel FQ
Published: January 2024

Experimentally evolving yeast to adhere better to plastic led to adaptations that increased their ability to cause an infection.
Contradiction in text review and apps rating: prediction using textual features and transfer learning
Authors: Aljrees Turki , Umer Muhammad , Saidani Oumaima , Almuqren Latifah , Ishaq Abid , Alsubai Shtwai , Eshmawi Ala’ Abdulmajid , Ashraf Imran
Published: January 2024

Mobile app stores, such as Google Play, have become famous platforms for practically all types of software and services for mobile phone users. Users may browse and download apps via app stores, which also help developers monitor their apps by allowing users to rate and review them. App reviews may contain the user’s experience, bug details, requests for additional features, or a textual rating of the app. These ratings can be frequently biased due to inadequate votes. However, there are significant discrepancies between the numerical ratings and the user reviews. This study uses a transfer learning approach to predict the numerical ratings of Google apps. It benefits from user-provided numeric ratings of apps as the training data and provides authentic ratings of mobile apps by analyzing users’ reviews. A transfer learning-based model ELMo is proposed for this purpose which is based on the word vector feature representation technique. The performance of the proposed model is compared with three other transfer learning and five machine learning models. The dataset is scrapped from the Google Play store which extracts the data from 14 different categories of apps. First, biased and unbiased user rating is segregated using TextBlob analysis to formulate the ground truth, and then classifiers prediction accuracy is evaluated. Results demonstrate that the ELMo classifier has a high potential to predict authentic numeric ratings with user actual reviews.
SOLEDAD Y FRAGILIDAD EN PERSONAS MAYORES QUE VIVEN EN COMUNIDAD: UNA REVISIÓN INTEGRATIVA
Authors: Carrasco Diaz Beatriz , Araya Alejandra-Ximena
Published: January 2024

CONTEXTO: Se proyecta un aumento del 6% en la población mundial de personas mayores para el 2050, generando desafíos sociales significativos, especialmente el crecimiento de la soledad en la vejez. Este fenómeno está estrechamente vinculado a problemas de salud notables, incluido el síndrome de fragilidad. OBJETIVO: Esta revisión de literatura tiene como objetivo proporcionar una comprensión actualizada de la relación entre fragilidad y soledad en adultos mayores que residen en la comunidad. METODOLOGÍA: Utilizando una revisión integradora, se recopilaron sistemáticamente artículos originales de las bases de datos de PubMed, Web of Science y CINAHL. RESULTADOS: Una revisión de 475 artículos publicados entre 2018 y 2022 identificó 17 estudios que cumplían con los criterios de inclusión y exclusión especificados. CONCLUSIÓN: Los hallazgos revelan sólida evidencia científica que respalda una asociación sustancial entre la soledad y la fragilidad en adultos mayores que viven en la comunidad. A pesar de la consistencia en la evidencia, persisten variaciones en los enfoques conceptuales y las metodologías de medición en los estudios revisados.
Informações ambientais complementares para a comunidade do entorno da usina siderúrgica, localizada na cidade de Volta Redonda, estado do Rio de Janeiro
Authors: Cabral Isabel , De Siqueira José Ricardo Maia , Ferreira Aracéli Cristina de Sousa
Published: January 2024

Os relatórios sociais e ambientais ainda não são obrigatórios para todas as empresas e não são submetidos ao processo de auditoria, portanto, geram dúvidas quanto ao seu conteúdo, já que os gestores podem escolher quais informações divulgar interferindo na qualidade e transparência dos relatórios. As formas tradicionais de contabilidade que salientam as perspectivas do mercado de capitais e dos acionistas, acabam por promover políticas neoliberais, mas esquecem o sentido democrático de participação. Para abdicar dessa abordagem monológica tradicional da contabilidade e buscar o aumento da participação democrática é necessário uma abordagem dialógica possibilitando o processo participativo que concilia as posições opostas, promove mudança social, reduz a assimetria de poder e, principalmente, dá voz aos tradicionalmente excluídos. Este artigo tem como objetivo desenvolver informações ambientais complementares direcionadas à comunidade do entorno da usina siderúrgica, localizada na cidade de Volta Redonda, Estado do Rio de Janeiro. Após a análise das entrevistas, foram elaborados dez indicadores ambientais complementares. A maior preocupação dos respondentes da comunidade local é a poluição atmosférica causada pelas atividades operacionais da usina siderúrgica. Os dez indicadores que estão sugeridos neste trabalho não conseguem captar a dor das pessoas, a angústia de viver sabendo que vai adoecer pela poluição, a mágoa pelo descaso da empresa e dos governos. Os resultados desta pesquisa são relevantes para os órgãos reguladores por mostrar as demandas da comunidade local que podem ser incorporadas aos relatórios e às demonstrações contábeis.
Interplay of Islam and Economic Growth: Unveiling the Long-run Dynamics in Muslim and Non-muslim Countries
Authors: Quadri Faruq Umar , Olaniyi Oluwaseun Oladeji , Olaoye Olugbenga Olaposi
Published: January 2024

This study delves into the intricate relationship between Islam and economic growth, examining how Islamic teachings impact economic policies and developmental outcomes. Using data from 47 countries over the period 2010-2021, and to achieve our research objectives the CS-ARDL estimating technique by Chudik et al. was employed. The research reveals that Islam positively influences economic growth in Muslim countries in the long run, with a less significant impact in non-Muslim countries. The study emphasizes the role of Islamic education in shaping the behavior of the Muslim population and its subsequent impact on economic growth. Recommendations include prioritizing investment in Islamic education to enhance human capital, fostering long-term economic plans that align with the positive influence of Islamic teachings, and leveraging education to contribute to sustained economic growth.
The Role of Social Media Platforms and Online Initiatives in Addressing and Creating Awareness about Mental Health Problems in the Youth and Adult Population
Authors: Shalaby Noha
Published: January 2024

In recent years, the role of social media platforms and online initiatives in addressing and creating awareness about mental health problems has become increasingly significant. There is no doubt about the fact that social media platforms play a very important role in destigmatizing mental health issues. Through personal narratives, testimonials, and awareness campaigns, individuals can openly discuss their experiences, challenging pre-existing stigmas and fostering a more empathetic understanding of mental health challenges. Online initiatives contribute to the accessibility of mental health information. From educational resources to coping strategies, individuals can readily access a wealth of information, empowering them to make informed decisions about their mental health. Social media facilitates the creation of online communities centered around mental health support. These virtual spaces connect individuals facing similar challenges, providing a supportive environment for sharing experiences, resources, and coping mechanisms. Online initiatives, such as forums and support groups, contribute to building a sense of community among those navigating mental health issues. The youth, being prolific users of social media, are directly influenced by online content. Digital campaigns and initiatives designed for and by the youth contribute to engagement and empowerment. Through interactive content, discussions, and awareness challenges, social media becomes a powerful tool for reaching and resonating with younger demographics. This research study examines the various ways in which social media platforms serve as catalysts for mental health discussions, destigmatization, and the provision of resources.