bluuebunny/crossref_metadata_2025 huggingface dataset:
The roles of traditional knowledge systems in orang-utanPongospp. and forest conservation: a case study of Danau Sentarum, West Kalimantan, Indonesia
Authors: Yuliani Elizabeth L. , Adnan Hasantoha , Achdiawan Ramadhani , Bakara Denny , Heri Valentinus , Sammy Jim , Salim M. Agus , Sunderland Terry
Published: November 2016

The orang-utanPongospp. is protected by national and international legislation, yet populations continue to decline. Many reports implicate local people in the poaching and illegal trade in orang-utans, yet community participation has been promoted as an alternative conservation strategy. To explore how community-based orang-utan conservation could be developed, we conducted a study to understand informal institutions, particularly local people's perceptions, traditional beliefs, taboos, norms and knowledge, related to orang-utan conservation within and around the wetlands of Danau Sentarum. The majority of Dayak communities interviewed practised traditional taboos, which supported the protection of orang-utans and their habitat. Statistical analysis using generalized linear modelling indicated that more orang-utan nests were found in areas with both good habitat condition and strong informal institutions. Despite applying traditional systems that are similar to conservation, local people have negative perceptions about the term ‘conservation’. We describe the underlying causes of these negative perceptions and highlight their implications for conservation programmes and policies. We conclude that conservation of orang-utans and other species should not focus on single species but on maintaining social and natural capital, cultural diversity and ecological functions at various institutional levels and across geographical scales.
Trade-offs between timber production, carbon stocking and habitat quality when managing woodlots for multiple ecosystem services
Authors: CARPENTIER SOPHIE , FILOTAS ELISE , HANDA I. TANYA , MESSIER CHRISTIAN
Published: November 2016

Managing for multiple ecosystem services is a growing issue for forest managers. As trade-offs arise between conflicting management objectives, stakeholders must be informed of the possible outcomes of alternative choices in order to facilitate decision-making. We modelled stand dynamics under single-management and functional zoning multiple-management (TRIAD; i.e. three-zone) scenarios in different forest types typical of eastern North America with the Forest Vegetation Simulator (FVS). Timber production, carbon stocking and habitat quality ecosystem services were calculated with simulation outputs. Habitat quality was measured using a habitat suitability index that integrated stand structural indicators. A multi-criteria decision analysis (MCDA) was performed in order to rank scenarios. We show that the most intensive management yielded greater timber volumes but resulted in the weakest carbon and habitat quality scores. The TRIAD scenarios in sugar maple–beech stands offered the best compromise in services compared to single management. In shade-intolerant deciduous stands, there was a loss of timber production with TRIAD scenarios, but greater carbon stock and habitat quality were observed. Our study contrasts alternative management scenarios for ecosystem services in woodlots of different forest types. It confirms that multiple harvest systems better achieve multiple services. The coupling of simulation modelling with MCDA offers a simple and flexible method to help stakeholders and managers make sound decisions.
Thoughts in the Field: ‘Self-reflexive narrative’ in field recording
Authors: Anderson Isobel , Rennie Tullis
Published: November 2016

This article considers the presence of ‘self-reflexive narrative’ in field recording. The authors interrogate a common presumption within sonic arts practice and sound studies discourse that field recordings represent authentic, impartial and neutral documents. Historically, field recording practice has not clearly represented narratives of how, when, why and by whom a field recording is made. In contrast, the social sciences have already experienced a narrative ‘turn’ since the 1970s, which highlighted the importance of recognising the presence and role of the researcher in the field, and also in representations of fieldwork. This provides an alternative framework for understanding field recording, in considering the importance of the recordist and their relationship with their recordings. Many sonic arts practitioners have already acknowledged that the subjective, personal qualities of field recording should be embraced, highlighted and even orated in their work. The authors’ own collaborative projectThoughts in the Fieldfurther explores these ideas, by vocalising ‘self-reflexive narratives’ in real time, within field recordings. The authors’ collaborative composition,Getting Lost(2015), demonstrates the compositional potentials this approach offers.
Acousmatic Foley: Staging sound-fiction
Authors: Pinheiro Sara
Published: November 2016

This article proposes a narrative theory thought in terms that are specific to sound practice. It addresses two different fields – Acousmatic Music and Foley Art – as a possibility of understanding sound narration and conceptualising it around the idea of fiction. To this end, it begins from the concepts of sound-motif, sound-prop and sound-actors, in order to propose a dramaturgic practice specific to sound terms.

The theory of sound dramaturgy acquires a practical outline by making use of multichannel constellations as a composition strategy, with specific loudspeaker arrangements. The theory advocates loudspeakers as the mediators of the experience and the stage as part of the audience’s assembly. This translates into a practice of staging sound fiction, which focuses on formulating a conjecture based on formal and factual structures, allowing for a direct relationship between the listener and the listening, between the sounds and their fictional location.
Narrative in Form: A topological study of meaning in transmedial narratives
Authors: Giannoukakis Marinos
Published: November 2016

This article is an attempt to demonstrate the relation between appreciation of morphology and structure in form on the one hand, with higher symbolic structures – crucial for meaning formation routines – on the other, and to evaluate their significance in transmedial narratives, primarily in the case of media-based artworks. The use of catastrophe theoretical models to classify forms, their structure and dynamics is proposed, and the question of how these models can give us insight into the meaning that is carried through transmedial narratives (referential or abstract) is examined. Finally, the value of these insights for the composition and practice-based analysis of multimedia art forms is demonstrated.
Conflicting Constraints in the Comparative Cycle
Authors: Reinarz Lukas , de Vos Hugo , de Hoop Helen
Published: November 2016

Present-day Dutch shows an alternation reflecting a transition from the use of the comparative particledan‘than’ toward the particleals‘as’ in comparisons of inequality. We argue that this transition—as well as the replacement ofdenn/dannbyalsin Early New High German, andals‘as’ bywie‘how’ in present-day German—results from a conflict between two competing principles, Economy and Iconicity. The conflict between these two constraints in German and Dutch gives rise to a cycle, in which having two particles—one for each construction—and having one particle for both, comparatives and equatives, alternate over time.*
BeyondDo-Support andTun-Periphrasis: The Case of Finite Verb Doubling in Karrharde North Frisian
Authors: Hoekstra Jarich
Published: November 2016

All West Germanic languages possess periphrastic verb constructions in which a finite dummy auxiliary ‘do’ combines with an infinitival thematic verb (comparedo-support in English andtun-periphrasis in German). In Frisian, periphrastic verb constructions are not very common. It is all the more surprising, therefore, to find a general periphrastic verb construction in Karrharde North Frisian that seems to go beyond the typology of these constructions in West Germanic to some extent: The construction is rather unconstrained, it features a mysterious dummy auxiliarywer- and, most strikingly, both the dummy auxiliary and the thematic verb are finite. In this article, the basic data on finite verb doubling in Karrharde North Frisian is presented, and the origin of the dummy auxiliarywer- is tracked down. A synchronic analysis of the construction is proposed that relates it to the periphrastic verb constructions in other West Germanic languages. It is shown that finite verb doubling is in most respects a garden variety periphrastic verb construction, and that its special properties can be traced back to the fact that the dummy auxiliary developed from the complementizerwer‘if, whether’ (possibly under language contact with Danish).
On the Distribution and Acquisition of the West Germanic ‘Need’-Verbs: German between Dutch and English
Authors: Lin Jing
Published: November 2016

This paper investigates three verbs expressing necessity in the three West Germanic languages: Dutchhoeven, Englishneed, and Germanbrauchen. These three verbs are all categorized as negative polarity items(npis). However, there are differences in their distribution as NPIs, which posit Germanbrauchenbetween Englishneedand Dutchhoeven.By analyzing two factors that may influence acquisition, namely, opacity and input frequency, this paper moreover presents a similar pattern for the acquisition of these NPIs: The Dutch NPIhoevenemerges earlier in child language than its German counterpart, which in turn arises earlier than the English NPIneed.
Skeleton growth under uniformly distributed force conditions: producing spherical sea urchins
Authors: Cheng Polly , Kambli Ankita , Stone Johnny
Published: November 2016

Sea urchin skeletons, or tests, comprise rigid calcareous plates, interlocked and sutured together with collagen fibres. The tests are malleable due to mutability in the collagen fibres that loosen during active feeding, yielding interplate gaps. We designed an extraterrestrial simulation experiment wherein we subjected actively growing sea urchins toone factorassociated with zero-gravity environments, by growing them under conditions in which reactionary gravitational forces were balanced, and observed how their tests responded. Preventing tests from adhering to surfaces during active growth produced more-spherical bodies, realized as increased height-to-diameter ratios. Sea urchin tests constitute ideal systems for obtaining data that could be useful in extraterrestrial biology research, particularly in how skeletons grow under altered-gravity conditions.
Minister and Viceroy, Paisano and Amigo: The Private Correspondence of the Marqués de la Ensenada and the Conde de Superunda, 1745–1749
Authors: Pearce Adrian
Published: November 2016

In the Archivo General de Indias in Seville there exists a remarkable sample of private correspondence between two of the most powerful figures of the Bourbon Age in Spanish American history. The principal writer is Zenón de Somodevilla y Bengoechea, marqués de la Ensenada and minister of Hacienda, War, and Indies and Marine since 1743, and subsequently holder of other posts that made him, in a contemporary opinion, “Secretary of Everything.” His correspondent is José Antonio Manso de Velasco, conde de Superunda and viceroy of Peru from 1745 to 1761, the longest serving and among the most influential of the Peruvian viceroys. The Seville correspondence consists mainly of just seven generally brief letters. The private character of these letters, however, along with the close relationship between the two men and the roles they occupied, makes them historically fascinating. Excerpts are published here for the first time, in both Spanish and English translation.
Space and Motion Stimulus-Response Correspondence (SRC) Effects in a Single Task
Authors: Styrkowiec Piotr
Published: November 2016

Abstract. Previous research indicated that congruency between stimulus location and response position (spatial stimulus-response correspondence [SRC]) and stimulus motion and response movement congruency (motion SRC) are distinct SRC phenomena. This study further explored this issue and tested whether these two SRC effects are independent. This was conducted by investigating these two SRC effects in a single task. A stimulus with leftward or rightward motion was presented on the left or the right side of the screen and the participant had to move the joysticks held with the left and right hands leftward or rightward in response to the stimulus color. In this setting, the stimulus and response shared two types of correspondence: spatial and motion. The results demonstrated that two SRC effects occurred and interacted (interaction evident only in reaction times [RTs]). RT distribution analysis and accuracy delta plots for each SRC effect indicated that spatial and motion SRC are distinct phenomena based on different processes.
How Did Climate and Humans Respond to Past Volcanic Eruptions?
Authors: Toohey Matthew , Ludlow Francis , LeGrande Allegra
Published: November 2016

First workshop of the Volcanic Impacts on Climate and Society Working Group; Palisades, New York, 6–8 June 2016
Continuous injection synthesis of indium arsenide quantum dots emissive in the short-wavelength infrared
Authors: Franke Daniel , Harris Daniel K. , Chen Ou , Bruns Oliver T. , Carr Jessica A. , Wilson Mark W. B. , Bawendi Moungi G.
Published: November 2016

With the emergence of applications based on short-wavelength infrared light, indium arsenide quantum dots are promising candidates to address existing shortcomings of other infrared-emissive nanomaterials. However, III–V quantum dots have historically struggled to match the high-quality optical properties of II–VI quantum dots. Here we present an extensive investigation of the kinetics that govern indium arsenide nanocrystal growth. Based on these insights, we design a synthesis of large indium arsenide quantum dots with narrow emission linewidths. We further synthesize indium arsenide-based core-shell-shell nanocrystals with quantum yields up to 82% and improved photo- and long-term storage stability. We then demonstrate non-invasive through-skull fluorescence imaging of the brain vasculature of murine models, and show that our probes exhibit 2–3 orders of magnitude higher quantum yields than commonly employed infrared emitters across the entire infrared camera sensitivity range. We anticipate that these probes will not only enable new biomedical imaging applications, but also improved infrared nanocrystal-LEDs and photon-upconversion technology.
Biocatalytic trifluoromethylation of unprotected phenols
Authors: Simon Robert C. , Busto Eduardo , Richter Nina , Resch Verena , Houk Kendall N. , Kroutil Wolfgang
Published: November 2016

Organofluorine compounds have become important building blocks for a broad range of advanced materials, polymers, agrochemicals, and increasingly for pharmaceuticals. Despite tremendous progress within the area of fluorination chemistry, methods for the direct introduction of fluoroalkyl-groups into organic molecules without prefunctionalization are still highly desired. Here we present a concept for the introduction of the trifluoromethyl group into unprotected phenols by employing a biocatalyst (laccase), tBuOOH, and either the Langlois’ reagent or Baran’s zinc sulfinate. The method relies on the recombination of two radical species, namely, the phenol radical cation generated directly by the laccase and the CF3-radical. Various functional groups such as ketone, ester, aldehyde, ether and nitrile are tolerated. This laccase-catalysed trifluoromethylation proceeds under mild conditions and allows accessing trifluoromethyl-substituted phenols that were not available by classical methods.
Spin injection and inverse Edelstein effect in the surface states of topological Kondo insulator SmB6
Authors: Song Qi , Mi Jian , Zhao Dan , Su Tang , Yuan Wei , Xing Wenyu , Chen Yangyang , Wang Tianyu , Wu Tao , Chen Xian Hui , Xie X. C. , Zhang Chi , Shi Jing , Han Wei
Published: November 2016

There has been considerable interest in exploiting the spin degrees of freedom of electrons for potential information storage and computing technologies. Topological insulators (TIs), a class of quantum materials, have special gapless edge/surface states, where the spin polarization of the Dirac fermions is locked to the momentum direction. This spin–momentum locking property gives rise to very interesting spin-dependent physical phenomena such as the Edelstein and inverse Edelstein effects. However, the spin injection in pure surface states of TI is very challenging because of the coexistence of the highly conducting bulk states. Here, we experimentally demonstrate the spin injection and observe the inverse Edelstein effect in the surface states of a topological Kondo insulator, SmB6. At low temperatures when only surface carriers are present, a clear spin signal is observed. Furthermore, the magnetic field angle dependence of the spin signal is consistent with spin–momentum locking property of surface states of SmB6.
Generation of mutation hotspots in ageing bacterial colonies
Authors: Sekowska Agnieszka , Wendel Sofie , Fischer Emil C. , Nørholm Morten H. H. , Danchin Antoine
Published: November 2016

How do ageing bacterial colonies generate adaptive mutants? Over a period of two months, we isolated on ageing colonies outgrowing mutants able to use a new carbon source, and sequenced their genomes. This allowed us to uncover exquisite details on the molecular mechanism behind their adaptation: most mutations were located in just a few hotspots in the genome, and over time, mutations increasingly were consistent with the involvement of 8-oxo-guanosine, formed exclusively on the transcribed strand. This work provides strong support for retromutagenesis as a general process creating adaptive mutations during ageing.
Erratum: Activation of P2X7 and P2Y11 purinergic receptors inhibits migration and normalizes tumor-derived endothelial cells via cAMP signaling
Authors: Avanzato D. , Genova T. , Fiorio Pla A. , Bernardini M. , Bianco S. , Bussolati B. , Mancardi D. , Giraudo E. , Maione F. , Cassoni P. , Castellano I. , Munaron L.
Published: November 2016

Scientific Reports 6: Article number: 32602; published online: 02 September 2016; updated: 11 November 2016
Photo-electrons unveil topological transitions in graphene-like systems
Authors: Peralta Gavensky Lucila , Usaj Gonzalo , Balseiro C. A.
Published: November 2016

The topological structure of the wavefunctions of particles in periodic potentials is characterized by the Berry curvature Ωkn whose integral on the Brillouin zone is a topological invariant known as the Chern number. The bulk-boundary correspondence states that these numbers define the number of edge or surface topologically protected states. It is then of primary interest to find experimental techniques able to measure the Berry curvature. However, up to now, there are no spectroscopic experiments that proved to be capable to obtain information on Ωkn to distinguish different topological structures of the bulk wavefunctions of semiconducting materials. Based on experimental results of the dipolar matrix elements for graphene, here we show that ARPES experiments with the appropriate x-ray energies and polarization can unambiguously detect changes of the Chern numbers in dynamically driven graphene and graphene-like materials opening new routes towards the experimental study of topological properties of condensed matter systems.
Elucidating a molecular mechanism that the deterioration of porcine meat quality responds to increased cortisol based on transcriptome sequencing
Authors: Wan Xuebin , Wang Dan , Xiong Qi , Xiang Hong , Li Huanan , Wang Hongshuai , Liu Zezhang , Niu Hongdan , Peng Jian , Jiang Siwen , Chai Jin
Published: November 2016

Stress response is tightly linked to meat quality. The current understanding of the intrinsic mechanism of meat deterioration under stress is limited. Here, male piglets were randomly assigned to cortisol and control groups. Our results showed that when serum cortisol level was significantly increased, the meat color at 1 h postmortem, muscle bundle ratio, apoptosis rate, and gene expression levels of calcium channel and cell apoptosis including SERCA1, IP3R1, BAX, Bcl-2, and Caspase-3, were notably increased. However, the value of drip loss at 24 h postmortem and serum CK were significantly decreased. Additionally, a large number of differentially expressed genes (DEGs) in GC regulation mechanism were screened out using transcriptome sequencing technology. A total of 223 DEGs were found, including 80 up-regulated genes and 143 down-regulated genes. A total of 204 genes were enriched in GO terms, and 140 genes annotated into in KEGG database. Numerous genes were primarily involved in defense, inflammatory and wound responses. This study not only identifies important genes and signalling pathways that may affect the meat quality but also offers a reference for breeding and feeding management to provide consumers with better quality pork products.
Transcriptome assists prognosis of disease severity in respiratory syncytial virus infected infants
Authors: Jong Victor L. , Ahout Inge M. L. , van den Ham Henk-Jan , Jans Jop , Zaaraoui-Boutahar Fatiha , Zomer Aldert , Simonetti Elles , Bijl Maarten A. , Brand H. Kim , van IJcken Wilfred F. J. , de Jonge Marien I. , Fraaij Pieter L. , de Groot Ronald , Osterhaus Albert D. M. E. , Eijkemans Marinus J. , Ferwerda Gerben , Andeweg Arno C.
Published: November 2016

Respiratory syncytial virus (RSV) causes infections that range from common cold to severe lower respiratory tract infection requiring high-level medical care. Prediction of the course of disease in individual patients remains challenging at the first visit to the pediatric wards and RSV infections may rapidly progress to severe disease. In this study we investigate whether there exists a genomic signature that can accurately predict the course of RSV. We used early blood microarray transcriptome profiles from 39 hospitalized infants that were followed until recovery and of which the level of disease severity was determined retrospectively. Applying support vector machine learning on age by sex standardized transcriptomic data, an 84 gene signature was identified that discriminated hospitalized infants with eventually less severe RSV infection from infants that suffered from most severe RSV disease. This signature yielded an area under the receiver operating characteristic curve (AUC) of 0.966 using leave-one-out cross-validation on the experimental data and an AUC of 0.858 on an independent validation cohort consisting of 53 infants. A combination of the gene signature with age and sex yielded an AUC of 0.971. Thus, the presented signature may serve as the basis to develop a prognostic test to support clinical management of RSV patients.
Herb-target interaction network analysis helps to disclose molecular mechanism of traditional Chinese medicine
Authors: Liang Hao , Ruan Hao , Ouyang Qi , Lai Luhua
Published: November 2016

Though many studies have been performed to elucidate molecular mechanism of traditional Chinese medicines (TCMs) by identifying protein-compound interactions, no systematic analysis at herb level was reported. TCMs are prescribed by herbs and all compounds from a certain herb should be considered as a whole, thus studies at herb level may provide comprehensive understanding of TCMs. Here, we proposed a computational strategy to study molecular mechanism of TCM at herb level and used it to analyze a TCM anti-HIV formula. Herb-target network analysis was carried out between 17 HIV-related proteins and SH formula as well as three control groups based on systematic docking. Inhibitory herbs were identified and active compounds enrichment was found to contribute to the therapeutic effectiveness of herbs. Our study demonstrates that computational analysis of TCMs at herb level can catch the rationale of TCM formulation and serve as guidance for novel TCM formula design.
Intrapleural Adenoviral-mediated Endothelial Cell Protein C Receptor Gene Transfer Suppresses the Progression of Malignant Pleural Mesothelioma in a Mouse Model
Authors: Keshava Shiva , Rao L. Vijaya Mohan , Pendurthi Usha R.
Published: November 2016

Malignant pleural mesothelioma (MPM) is an aggressive thoracic cancer with a high mortality rate as it responds poorly to standard therapeutic interventions. Our recent studies showed that expression of endothelial cell protein C receptor (EPCR) in MPM cells suppresses tumorigenicity. The present study was aimed to investigate the mechanism by which EPCR suppresses MPM tumor growth and evaluate whether EPCR gene therapy could suppress the progression of MPM in a mouse model of MPM. Measurement of cytokines from the pleural lavage showed that mice implanted with MPM cells expressing EPCR had elevated levels of IFNγ and TNFα compared to mice implanted with MPM cells lacking EPCR. In vitro studies demonstrated that EPCR expression renders MPM cells highly susceptible to IFNγ + TNFα-induced apoptosis. Intrapleural injection of Ad.EPCR into mice with an established MPM originating from MPM cells lacking EPCR reduced the progression of tumor growth. Ad.EPCR treatment elicited recruitment of macrophages and NK cells into the tumor microenvironment and increased IFNγ and TNFα levels in the pleural space. Ad.EPCR treatment resulted in a marked increase in tumor cell apoptosis. In summary, our data show that EPCR expression in MPM cells promotes tumor cell apoptosis, and intrapleural EPCR gene therapy suppresses MPM progression.
Speeding up GW Calculations to Meet the Challenge of Large Scale Quasiparticle Predictions
Authors: Gao Weiwei , Xia Weiyi , Gao Xiang , Zhang Peihong
Published: November 2016

Although the GW approximation is recognized as one of the most accurate theories for predicting materials excited states properties, scaling up conventional GW calculations for large systems remains a major challenge. We present a powerful and simple-to-implement method that can drastically accelerate fully converged GW calculations for large systems, enabling fast and accurate quasiparticle calculations for complex materials systems. We demonstrate the performance of this new method by presenting the results for ZnO and MgO supercells. A speed-up factor of nearly two orders of magnitude is achieved for a system containing 256 atoms (1024 valence electrons) with a negligibly small numerical error of ±0.03 eV. Finally, we discuss the application of our method to the GW calculations for 2D materials.
Therapeutic efficacy and safety of S-1-based combination therapy compare with S-1 monotherapy following gemcitabine failure in pancreatic cancer: a meta-analysis
Authors: Lu Sinan , Zhang Yuan , Zhou Xiaohu , Zhou Dongkai , Yang Qifan , Ju Bingjie , Zhao Xinyi , Hu Zhenhua , Xie Haiyang , Zhou Lin , Zheng Shusen , Wang Weilin
Published: November 2016

S-1 monotherapy is widely used following gemcitabine failure in pancreatic cancer, especially in East Asia. We performed a meta-analysis to determine whether S-1-based combination therapy had better efficacy and safety compared with S-1 monotherapy. We searched Pubmed, Web of Science, ClinicalTrials.gov, and Cochrane CENTRAL and subsequently included five trials with a total of 690 patients. The combined hazard ratio (HR) or risk ratio; the corresponding 95% confidence intervals of progression-free survival, overall survival, and overall response rate; and grade 3–4 adverse events were examined. Five randomized controlled trials were included. Meta-analysis demonstrated S-1-based combination therapy significantly increased progression-free survival (HR = 0.78, 95% confidence interval [CI]: 0.67–0.90, p = 0.0009) and overall response rate (HR = 1.74, 95% CI: 1.20–2.52, p = 0.003). Evidence was insufficient to confirm that S-1-based combined regimens improved overall survival (HR = 0.87, 95% CI: 0.75–1.00, p = 0.05). There was no significant difference in adverse events between the two treatment arms. In conclusion, S-1-based combination therapy improved progression-free survival and overall response rate compared to S-1 monotherapy with acceptable toxicity.
Adenovirus vector-based incorporation of a photo-cross-linkable amino acid into proteins in human primary cells and cancerous cell lines
Authors: Kita Ayami , Hino Nobumasa , Higashi Sakiko , Hirota Kohji , Narumi Ryohei , Adachi Jun , Takafuji Kazuaki , Ishimoto Kenji , Okada Yoshiaki , Sakamoto Kensaku , Tomonaga Takeshi , Takashima Seiji , Mizuguchi Hiroyuki , Doi Takefumi
Published: November 2016

The site-specific incorporation of cross-linkable designer amino acids into proteins is useful for covalently bonding protein complexes upon exposure to light. This technology can be used to study networks of protein-protein interactions in living cells; however, to date it has only been applicable for use with a narrow range of cell types, due to the limited availability of plasmid-based transfection protocols. In the present study, we achieved adenovirus-based expression of a variant of an archaeal pyrrolysyl-tRNA synthetase and UAG-recognising tRNA pair, which was used to incorporate unnatural amino acids into proteins at sites defined by in-frame UAG codons within genes. As such, the site-specific photo-cross-linking method is now applicable to a wide variety of mammalian cells. In addition, we repositioned the reactive substituent of a useful photo-cross-linker, Nε-(para-trifluoromethyl-diazirinyl-benzyloxycarbonyl)-l-lysine (pTmdZLys), to the meta position, which improved its availability at low concentration. Finally, we successfully applied this system to analyse the formation of a protein complex in response to a growth signal in human cancerous cells and human umbilical vein endothelial cells. This adenovirus-based system, together with the newly designed cross-linkable amino acid, will facilitate studies on molecular interactions in various cell lines of medical interest.
A simple method to improve the stability of docetaxel micelles
Authors: Zhang Lan , Tan LiWei , Chen LiJuan , Chen XiaoXin , Long ChaoFeng , Peng JinRong , Qian ZhiYong
Published: November 2016

Self-assembled polymeric micelles have been widely applied in drug delivery systems. In this study, we found that pH value of micellar system solution was the decisive factor of physical stability. Furthermore, the weak basic solution could maintain the solution clarification for a relative long time. To investigate the stability of polymeric micelles in different pH solutions, the micellar particle size and the docetaxel content remaining in solution were detected at predetermined time points. The crystallographic assay of freeze-drying powder was characterized by an X-ray diffractometer. In vitro release results indicated that the PBS had little influence on the sustained-release effect of docetaxel-loaded polymeric micelles (DPM). Besides, the safety of micellar formulation was determined by an MTT assay on HEK293 cells, and the anti-tumor activity was tested on MCF-7 cells. The results demonstrated that DPM adjusted with PBS (DPM (PBS)) was of low toxicity and maintained the effectiveness of docetaxel. In vivo antitumor results indicated that DPM (PBS) had better antitumor efficacy than common docetaxel injection (DTX). Thus it was concluded that regulation of micellar solution PH by PBS is a safe and effective method to improve the physical stability of DPM. It might promote the application of micellar formulation in clinical applications.
Long intergenic non-coding RNA 271 is predictive of a poorer prognosis of papillary thyroid cancer
Authors: Ma Ben , Liao Tian , Wen Duo , Dong Chuanpeng , Zhou Li , Yang Shuwen , Wang Yu , Ji Qinghai
Published: November 2016

A number of long non-coding RNAs (lncRNAs) have been found to play critical roles in oncogenesis and tumor progression. We aimed to investigate whether lncRNAs could act as prognostic biomarkers for papillary thyroid cancer (PTC) that may assist us in evaluating disease status and prognosis for patients. We found 220 lncRNAs with expression alteration from the annotated 2773 lncRNAs approved by the HUGO gene nomenclature committee in The Cancer Genome Atlas (TCGA) dataset, of which FAM41C, CTBP1-AS2, LINC00271, HAR1A, LINC00310 and HAS2-AS1 were associated with recurrence. After adjusting classical clinicopathogical factors and BRAFV600E mutation, LINC00271 was found to be an independent risk factor for extrathyroidal extension, lymph node metastasis, advanced tumor stage III/IV and recurrence in multivariate analyses. Additionally, LINC00271 expression was significantly downregulated in PTCs versus adjacent normal tissues (P < 0.001). The Gene Set Enrichment Analysis (GSEA) revealed that genes associated with cell adhesion molecules, cell cycle, P53 signaling pathway and JAK/STAT signaling pathway were remarkably enriched in lower-LINC00271 versus higher-LINC00271 tumors. In conclusion, LINC00271 was identified as a possible suppressor gene in PTC in our study, and it may serve as a potential predictor of poor prognoses in PTC.
Genetic and pharmacological inhibition of CDK9 drives neutrophil apoptosis to resolve inflammation in zebrafish in vivo
Authors: Hoodless Laura J. , Lucas Christopher D. , Duffin Rodger , Denvir Martin A. , Haslett Christopher , Tucker Carl S. , Rossi Adriano G.
Published: November 2016

Neutrophilic inflammation is tightly regulated and subsequently resolves to limit tissue damage and promote repair. When the timely resolution of inflammation is dysregulated, tissue damage and disease results. One key control mechanism is neutrophil apoptosis, followed by apoptotic cell clearance by phagocytes such as macrophages. Cyclin-dependent kinase (CDK) inhibitor drugs induce neutrophil apoptosis in vitro and promote resolution of inflammation in rodent models. Here we present the first in vivo evidence, using pharmacological and genetic approaches, that CDK9 is involved in the resolution of neutrophil-dependent inflammation. Using live cell imaging in zebrafish with labelled neutrophils and macrophages, we show that pharmacological inhibition, morpholino-mediated knockdown and CRISPR/cas9-mediated knockout of CDK9 enhances inflammation resolution by reducing neutrophil numbers via induction of apoptosis after tailfin injury. Importantly, knockdown of the negative regulator La-related protein 7 (LaRP7) increased neutrophilic inflammation. Our data show that CDK9 is a possible target for controlling resolution of inflammation.
TRPC3 positively regulates reactive oxygen species driving maladaptive cardiac remodeling
Authors: Kitajima Naoyuki , Numaga-Tomita Takuro , Watanabe Masahiko , Kuroda Takuya , Nishimura Akiyuki , Miyano Kei , Yasuda Satoshi , Kuwahara Koichiro , Sato Yoji , Ide Tomomi , Birnbaumer Lutz , Sumimoto Hideki , Mori Yasuo , Nishida Motohiro
Published: November 2016

Reactive oxygen species (ROS) produced by NADPH oxidase 2 (Nox2) function as key mediators of mechanotransduction during both physiological adaptation to mechanical load and maladaptive remodeling of the heart. This is despite low levels of cardiac Nox2 expression. The mechanism underlying the transition from adaptation to maladaptation remains obscure, however. We demonstrate that transient receptor potential canonical 3 (TRPC3), a Ca2+-permeable channel, acts as a positive regulator of ROS (PRROS) in cardiomyocytes, and specifically regulates pressure overload-induced maladaptive cardiac remodeling in mice. TRPC3 physically interacts with Nox2 at specific C-terminal sites, thereby protecting Nox2 from proteasome-dependent degradation and amplifying Ca2+-dependent Nox2 activation through TRPC3-mediated background Ca2+ entry. Nox2 also stabilizes TRPC3 proteins to enhance TRPC3 channel activity. Expression of TRPC3 C-terminal polypeptide abolished TRPC3-regulated ROS production by disrupting TRPC3-Nox2 interaction, without affecting TRPC3-mediated Ca2+ influx. The novel TRPC3 function as a PRROS provides a mechanistic explanation for how diastolic Ca2+ influx specifically encodes signals to induce ROS-mediated maladaptive remodeling and offers new therapeutic possibilities.
Cytokines as the good predictors of refractory Mycoplasma pneumoniae pneumonia in school-aged children
Authors: Zhang Yuanyuan , Mei Shufen , Zhou Yunlian , Huang Meixia , Dong Guijuan , Chen Zhimin
Published: November 2016

Excessive immune response against pathogens may play an important role in refracory Mycoplasma pneumoniae pneumonia (RMPP). The aim of this study was to elucidate the associations between cytokines and the prediction of RMPP in school-aged patients. Retrospective analysis was performed on school-aged children with Mycoplasma pneumoniae pneumonia (MPP) hospitalized in our hospital between January 1, 2011 and December 31, 2015. The clinical charcteristics, including the cytokines in serum between the RMPP group and the general Mycoplasma pneumoniae pneumonia (GMPP) group were compared and the predictive values of RMPP were explored. Of total 180 patients, 115 patients were in the GMPP group, 65 were in the RMPP group. We found the levels of cytokines, including nterleukin (IL)-6, IL-10, interferon gamma (IFN-γ) in RMPP group were significantly higher than those in GMPP group (P < 0.01). In ROC curve analysis, IL-10 and IFN-γ were useful for differentiating patients with RMPP from those with GMPP. Logistic regression analysis showed that the IL-10 ≥ 3.65 pg/ml and IFN-γ ≥ 29.05 pg/ml were significant predictors regarding to RMPP. Additionally, a positive correlation between serum IL-10 and IFN-γ concentrations was observed. Conclusions: IL-10 and IFN-γ could be used as the good predictors of RMPP in school-aged children.
Demonstration of Tunable Steering and Multiplexing of Two 28 GHz Data Carrying Orbital Angular Momentum Beams Using Antenna Array
Authors: Xie Guodong , Zhao Zhe , Yan Yan , Li Long , Ren Yongxiong , Ahmed Nisar , Cao Yinwen , Willner Asher J. , Bao Changjing , Wang Zhe , Liu Cong , Ziyadi Morteza , Talwar Shilpa , Sajuyigbe Soji , Ashrafi Solyman , Tur Moshe , Molisch Andreas F. , Willner Alan E.
Published: November 2016

In line-of-sight communication systems, accurate alignment between the transmitter and receiver is important to guarantee sufficient signal power at the receiver. Such alignment is even more important for orbital angular momentum (OAM) multiplexing systems since misalignment between the transmitter and receiver may cause crosstalk among channels. In this paper, we demonstrate the simultaneous generation and tunable steering of two OAM beams utilising a custom-designed circular antenna array at 28 GHz. We achieve a steering angle of up to 35 degrees from the antenna array normal. We find that (i) the steering angle of the generated OAM beams is limited by the emitting angle of the antenna elements, and (ii) a larger steering angle may degrade the mode purity of the generated OAM beams as well as induce inter-symbol-interference to each of the individual channels. Moreover, we demonstrate the transmission of two 1-Gbaud quadratic phase shift keying (QPSK) signal over the two steerable OAM beams with both multiplexed channels achieved bit error rates (BERs) of <3.8 × 10−3.
Identification of the impurities in 2,5-dimethoxy-4-ethylphenethylamine tablets by high performance liquid chromatography mass spectrometry-ion trap-time of flight
Authors: Li Yaning , Wang Minchao , Li Aoxin , Zheng Hui , Wei Yun
Published: November 2016

An efficient LC/MS-IT-TOF method to identify ten impurities and fragment pathways in 2C-E tablets was established for the first time.
Validation of density separation for the rapid recovery of microplastics from sediment
Authors: Quinn Brian , Murphy Fionn , Ewins Ciaran
Published: November 2016

Validation of the separation of secondary microplastics from sediment using numerous brine solutions in a rapid, reproducible, single stage technique.
Self-assembling nanowires of an amphiphilic camptothecin prodrug derived from homologous derivative conjugation
Authors: He Dongxuan , Zhang Wei , Deng Hongzhang , Huo Shuaidong , Wang Yi-Feng , Gong Ningqiang , Deng Liandong , Liang Xing-Jie , Dong Anjie
Published: November 2016

A novel amphiphilic camptothecin prodrug, CPT-ss-Ir, was synthesized and used to construct self-assembled nanowires, which could release active CPT and Ir species upon intracellular triggering.
Highly potent extranuclear-targeted luminescent iridium(iii) antitumor agents containing benzimidazole-based ligands with a handle for functionalization
Authors: Yellol Jyoti , Pérez Sergio A. , Yellol Gorakh , Zajac Juraj , Donaire Antonio , Vigueras Gloria , Novohradsky Vojtech , Janiak Christoph , Brabec Viktor , Ruiz José
Published: November 2016

This discovery could open the door to a new large family of multifunctional bioconjugated drugs.
Cellular delivery of enzyme-loaded DNA origami
Authors: Ora Ari , Järvihaavisto Erika , Zhang Hongbo , Auvinen Henni , Santos Hélder A. , Kostiainen Mauri A. , Linko Veikko
Published: November 2016

Enzyme-loaded DNA origamis are transported into cells, and the activity of the delivered enzymes is determined via luminescence assay.
Synthesis of ramariolide natural products and discovery of their targets in mycobacteria
Authors: Lehmann Johannes , Richers Johannes , Pöthig Alexander , Sieber Stephan A.
Published: November 2016

The total synthesis of ramariolides A–D and ABPP (photoprobe) derivatives have been synthesized for target identification in mycobacterial strains.
Scaling relationships for nonadiabatic energy relaxation times in warm dense matter: toward understanding the equation of state
Authors: Pradhan Ekadashi , Magyar Rudolph J. , Akimov Alexey V.
Published: November 2016

The dependence of nonadiabatic ion-electron energy transfer rates in warm dense aluminum on the mass density and temperature with decoherence changing this relationship qualitatively.
Low-temperature H2-plasma-assisted NOx storage and reduction over a combined Pt/Ba/Al and LaMnFe catalyst
Authors: Zhang Zhao-shun , Shi Chuan , Bai Zhi-feng , Li Ming-run , Chen Bing-bing , Crocker Mark
Published: November 2016

With the assistance of H2 plasma in rich phase, higher NOx conversions could be obtained over the PBA+LMF sample over a wide temperature range (200–350 °C).
Tuning of chain chirality by interchain stacking forces and the structure–property relationship in coordination systems constructed by meridional FeIII cyanide and MnIII Schiff bases
Authors: Sohn Ah Ram , Lim Kwang Soo , Kang Dong Won , Song Jeong Hwa , Koh Eui Kwan , Moon Dohyun , Hong Chang Seop
Published: November 2016

Cyanide-bridged Fe(iii)Mn(iii) coordination polymers with racemic arrangements show a correlation between the coupling constant and Mn–NC angle.
The synthesis and structure of a dianionic species with a bond between pentacoordinated tin atoms: bonding properties of the tin–tin bond
Authors: Tsukada Satoru , O'Brien Nathan J. , Kano Naokazu , Kawashima Takayuki , Guo Jing-Dong , Nagase Shigeru
Published: November 2016

The first dianionic compound bearing a bond between two pentacoordinated tin atoms, a distannate, was synthesized and crystallographically characterized.
Birnessite-type MnO2nanosheet arrays with interwoven arrangements on vapor grown carbon fibers as hybrid nanocomposites for pseudocapacitors
Authors: Sekhar S. Chandra , Nagaraju Goli , Cha Sung Min , Yu Jae Su
Published: November 2016

A cost-effective composite electrode featuring MnO2NSAs@VCFs exhibits good electrochemical performance as an electroactive material for pseudocapacitors.
Correction: Ga[OSi(OtBu)3]3·THF, a thermolytic molecular precursor for high surface area gallium-containing silica materials of controlled dispersion and stoichiometry
Authors: Dombrowski James P. , Johnson Gregory R. , Bell Alexis T. , Tilley T. Don
Published: November 2016

Correction for ‘Ga[OSi(OtBu)3]3·THF, a thermolytic molecular precursor for high surface area gallium-containing silica materials of controlled dispersion and stoichiometry’ by James P. Dombrowski et al., Dalton Trans., 2016, 45, 11025–11034.
Synergistic effects of mining and urban effluents on the level and distribution of methylmercury in a shallow aquatic ecosystem of the Bolivian Altiplano
Authors: Alanoca L. , Guédron S. , Amouroux D. , Audry S. , Monperrus M. , Tessier E. , Goix S. , Acha D. , Seyler P. , Point D.
Published: November 2016

Lake Uru Uru (3686 m a.s.l.) located in the Bolivian Altiplano region receives both mining effluents and urban wastewater discharges originating from the surrounding local cities which are under rapid development.
5-Hydroxymethyl-2-vinylfuran: a biomass-based solvent-free adhesive
Authors: Han Miaomiao , Liu Xiao , Zhang Xiaosa , Pang Yuanyuan , Xu Peng , Guo Jianwei , Liu Yadong , Zhang Shuangyan , Ji Shengxiang
Published: November 2016

5-Hydroxymethyl-2-vinylfuran (HMVF), a biomass-based solvent-free adhesive, exhibits strong adhesion to steel, copper, aluminum and glass.
Development of small molecular compounds targeting cancer stem cells
Authors: Park Jae-Hyun , Chung Suyoun , Matsuo Yo , Nakamura Yusuke
Published: November 2016

This review discusses potent molecular targets for cancer stem cells and recently developed small molecules against MELK, TOPK, and TTK.
Structure-guided optimization of quinoline inhibitors of Plasmodium N-myristoyltransferase
Authors: Goncalves Victor , Brannigan James A. , Laporte Alice , Bell Andrew S. , Roberts Shirley M. , Wilkinson Anthony J. , Leatherbarrow Robin J. , Tate Edward W.
Published: November 2016

Quinolines with balanced activities against bothPlasmodium vivaxandPlasmodium falciparum N-myristoyltransferase were identified.
Design, synthesis and anticonvulsant-analgesic activity of new N-[(phenoxy)alkyl]- and N-[(phenoxy)ethoxyethyl]aminoalkanols
Authors: Rapacz Anna , Waszkielewicz Anna M. , Pańczyk Katarzyna , Pytka Karolina , Koczurkiewicz Paulina , Piska Kamil , Pękala Elżbieta , Budziszewska Bogusława , Starek-Świechowicz Beata , Marona Henryk
Published: November 2016

New aminoalkanols have been synthesized and evaluated for their anticonvulsant activity in maximal electroshock (MES), maximal electroshock seizure threshold (MEST) and pentylenetetrazol (PTZ) tests, and show promising activity.
Influence of graphene oxide supports on solution-phase catalysis of thiolate-protected palladium nanoparticles in water
Authors: Chen Vivian , Pan Hanqing , Jacobs Roxanne , Derakhshan Shahab , Shon Young-Seok
Published: November 2016

The colloidal stability and catalytic activity of Pd nanoparticles are affected by the presence of graphene oxide and surface ligands.
Visible-light-induced phenylchalcogenyl-oxygenation of allenes having aryl or electron withdrawing substituents with ambient air as a sole oxidant
Authors: Kumaraswamy Gullapalli , Vijaykumar Swargam , Ankamma Kukkadapu , Narayanarao Vykunthapu
Published: November 2016

The stereoselective synthesis of α-phenylselenyl- or telluryl β-aryl acrylaldehydes/ketones from aryl allenes/1,3-diarylallene by an oxidative photo-induced single electron transfer process using benign ambient air as a sole oxidant was achieved.
Thermal stability enhancement of hydrogen bonded semicrystalline thermoplastics achieved by combination of aramide chemistry and supramolecular chemistry
Authors: Hayashi Mikihiro , Tournilhac Francois
Published: November 2016

Supramolecular polymers based on an amorphous fatty acid central block and crystallizable H-bonding end-groups of increasing size show low melt viscosity and tunable thermo-stability.
Correction: Anomalous red emission with competition and coexistence of defect and band edge emission in photo-electrochemically active (Zn0.97Ga0.03)(O0.95N0.05) solid solution
Authors: Menon Sumithra Sivadas , Sen Sayantani , Pramanik Pallabi , Bhattacharyya Anirban , Gupta Bhavana , Tiwari Brajesh , Baskar K. , Singh Shubra
Published: November 2016

Correction for ‘Anomalous red emission with competition and coexistence of defect and band edge emission in photo-electrochemically active (Zn0.97Ga0.03)(O0.95N0.05) solid solution’ by Sumithra Sivadas Menon et al., RSC Adv., 2016, 6, 103081–103087.