bluuebunny/crossref_metadata_2025 huggingface dataset:
Comparison of Drying Methods and Their Effect on the Stability of Graševina Grape Pomace Biologically Active Compounds
Authors: Sokač Tea , Gunjević Veronika , Pušek Anita , Tušek Ana Jurinjak , Dujmić Filip , Brnčić Mladen , Ganić Karin Kovačević , Jakovljević Tamara , Uher Darko , Mitrić Grozdana , Redovniković Ivana Radojčić
Published: January 2022

Valorisation of grape pomace, a by-product of the winery industry, has been pushed into the spotlight in recent years since it can enable lower environmental impact, but it can also bring an added value to the wine production process by recovering several grape pomace biologically active compounds. The first step that allows for grape pomace reuse is its drying, which should be carefully performed in order to preserve the biologically active compounds’ stability. In this study, the effects of different drying methods on the stability of polyphenols, tannins and tartaric acid in grape pomace (Vitis vinifera) cv. Graševina were investigated. In particular, vacuum drying (at different temperatures: 35, 50 and 70 °C), conventional drying at 70 °C and open sun drying were performed and the drying kinetics was described using Peleg’s model. Considering the processing time and thermodynamics, vacuum drying at 70 °C was the most convenient processing method. Polyphenols were highly stable during drying, and slight degradation occurred during vacuum drying at 35 and 50 °C. Tannins and tartaric acid were more prone to degradation depending on the drying method applied and showed the greatest stability during vacuum drying at 70 °C.
Geographical Origin Assessment of Extra Virgin Olive Oil via NMR and MS Combined with Chemometrics as Analytical Approaches
Authors: Calò Francesca , Girelli Chiara Roberta , Wang Selina C. , Fanizzi Francesco Paolo
Published: January 2022

Geographical origin assessment of extra virgin olive oil (EVOO) is recognised worldwide as raising consumers’ awareness of product authenticity and the need to protect top-quality products. The need for geographical origin assessment is also related to mandatory legislation and/or the obligations of true labelling in some countries. Nevertheless, official methods for such specific authentication of EVOOs are still missing. Among the analytical techniques useful for certification of geographical origin, nuclear magnetic resonance (NMR) and mass spectroscopy (MS), combined with chemometrics, have been widely used. This review considers published works describing the use of these analytical methods, supported by statistical protocols such as multivariate analysis (MVA), for EVOO origin assessment. The research has shown that some specific countries, generally corresponding to the main worldwide producers, are more interested than others in origin assessment and certification. Some specific producers such as Italian EVOO producers may have been focused on this area because of consumers’ interest and/or intrinsic economical value, as testified also by the national concern on the topic. Both NMR- and MS-based approaches represent a mature field where a general validation method for EVOOs geographic origin assessment could be established as a reference recognised procedure.
Methodological Approach to Designing Fermented Dairy Products with Optimal Biological Value
Authors: Zobkova Zinaida S. , Lazareva Ekaterina G. , Semipyatniy Vladislav K.
Published: January 2022

The development of food products implies ensuring the optimal composition and ratio of the basic components, as well as their technological compatibility. A priori, the quality of raw materials, the optimal formula of the product and the efficiency of the technological process determine the quality of products, including biological value. The use of whole-cell sensors such as infusoria Tetrahymena pyriformis is most productive for screening biological studies. At present, for a comprehensive assessment there are no data on the use of simplest technology of fermented dairy products and the design of their biological value. The purpose of this research is to develop a methodology for creating whole-milk products of optimal biological value using the express method of biotesting. The research object was yogurt with the ratio of the mass fraction of fat and protein in the range of 0.36 ÷ 1.5, sucrose in the range of 5 ÷ 10%. An express method for determining the relative biological value of fermented dairy products using test organisms and an original methodology for creating whole-milk products of optimal biological value have been developed. A software has been developed to calculate formula of the product optimized for the following indicators: the relative biological value of the product, the cost of raw material and basic materials. The methodology is a tool to assist industry organizations in improving production technologies and quality management systems.
HS-SPME Combined with GC-MS/O to Analyze the Flavor of Strong Aroma Baijiu Daqu
Authors: Wang Zhe , Wang Song , Liao Pengfei , Chen Lu , Sun Jinyuan , Sun Baoguo , Zhao Dongrui , Wang Bowen , Li Hehe
Published: January 2022

Daqu has gained wide attention because it is an essential source of microorganisms and flavor in baijiu production. In this study, HS-SPME combined with GC-MS/O was used to analyze the volatile flavor components of Strong aroma baijiu Daqu. DI-GC-O was used to choose the best extraction fiber to extract the representative overall aroma profile of Daqu. A total of 139 compounds were identified in the six different maturity stages of Daqu, and these compounds are of different types and concentrations. HS-SPME combined with GC-MS/O was used to analyze the aroma active substances in the finished Daqu, and a total of 43 aroma compounds were identified. The OAVs of 21 aromatic compounds were calculated based on the quantitative analysis results of MHS-SPME. Eighteen compounds with OAVs ≥ 1 made significant contributions to the overall aroma of Daqu, including guaiacol, 4-ethyl-2-methoxy phenol, 2-ethyl-3,5-dimethylpyrazine, etc.
Design of an Auxiliary Artificial Lymphatic Vessel in Treatment of Secondary Lymphedema Due to Breast Cancer
Authors: Durán-Aguilar Gabriela , Rossa-Sierra Alberto , Fuentes-Aguilar Rita Q.
Published: January 2022

Breast cancer is the most common malignant tumor that affects women in the United States, Europe, and Mexico. As an adverse effect when performing treatments for this condition, secondary lymphedema associated with breast cancer occurs in some cases. This complication occurs due to the interruption of lymphatic flow in the upper extremities in conjunction with other factors such as radiation, sedentary lifestyle, removal of lymph nodes, damage to lymphatic vessels, and others. This article reviews breast cancer incidence, mortality, and survival patterns, confirming that, specifically, lymphedema has high health, social, and economic impacts. Research demonstrates that it fundamentally affects women at an early age. In approximately a third of the cases, it becomes a chronic disease. Therefore, physical therapy is essential for a better quality of life in patients who survive this disease. Surgeries and manual and pharmacological treatments are the current procedures done to reduce to reduce the alterations suffered by patients with lymphedema; however, the success of the treatments depends on each patient’s characteristics. To face this problem, the design of a lymphatic vessel has been proposed to assist the mechanical failure of the damaged lymphatic system. In this work, the design methodology used for the blueprint of the lymphatic vessel is presented, as well as the computer analysis of fluid simulation and the selection of the proposed material, resulting in the production of a micrometric design. In the future, it is expected that a surgeon will be able to implant the design of the vessel to restore lymph flow through the lymphatic system, thus helping to combat lymphedema.
Experience of Late Miscarriage and Practical Implications for Post-Natal Health Care: Qualitative Study
Authors: Kukulskienė Milda , Žemaitienė Nida
Published: January 2022

Miscarriage is the most common reason for pregnancy loss, affecting around one in four pregnancies. It is classified as a traumatic event, associated with an increased risk for depression, anxiety, post-traumatic stress, alcohol dependence, somatic symptoms, sexual dysfunction, suicide, and complicated grief. This study aimed to analyse experiences of late miscarriage and to describe practical implications for post-natal health care based on characteristics of pregnancy loss revealed in a qualitative study. Seven women who had late miscarriages participated in in-depth biographical interviews. A phenomenological thematic analysis was applied. Experiential characteristics of late miscarriage were described by four themes and 13 subthemes: the initial splitting state (Dissociation, An Opened Void, An impaired Symbiosis, and The Body is Still Pregnant while the Psyche is Mourning); Betrayal of the body (Symbolic Experience of Internalised Death, Shocking Materiality of the Ongoing Miscarriage, Lost control of the Body, and Confusing Body Signals); Disconnecting (Depersonalising Medical Environment, Guilt Falsifies perception, and Retreat as a means of Self-Preservation); and Reconnecting (Collecting Shatters and Reinterpretation of Maternal Identity). Based on the results of the experiential analysis, another four themes represent practical recommendations for post-natal health care: Informing, Opportunity for Goodbye, Attention to Emotional Wellbeing, and Respectful Hospital Environment.
Understanding the Relationship between Fetal Alcohol Spectrum Disorder (FASD) and Criminal Justice: A Systematic Review
Authors: Sessa Francesco , Salerno Monica , Esposito Massimiliano , Di Nunno Nunzio , Li Rosi Giuseppe , Roccuzzo Salvatore , Pomara Cristoforo
Published: January 2022

Prenatal alcohol exposure is considered one of the main causes of preventable birth disorders; however, it represents the main form of developmental delay in the world. Among the so-called secondary disabilities related to fetal alcohol spectrum disorder (FASD), there is a close connection with criminal behavior. This systematic review aims to provide up-to-date information about the relationship between FASD subjects and criminal justice analyzing different aspects. In light of the results of this review, a further goal is to provide several suggestions in order to reduce the public cost impact of FASD. A systematic review of the literature was conducted according to the PRISMA guidelines, producing 20 articles that met the inclusion criteria. Based on the results published in the selected studies, fetal alcohol syndrome (FAS) is a leading cause of preventable birth disorders and developmental disabilities in newborns. Moreover, these subjects seem to be more inclined to criminal acts compared to others. In conclusion, it should be pointed out that FASD entails high public health costs, both regarding the support measures provided to the affected individual and to their family, as well as the cost and social impact of any criminal offenses committed.
Resolution of Resilience: Empirical Findings on the Challenges Faced and the Mitigation Strategies Adopted by Community Health Workers (CHWs) to Provide Maternal and Child Health (MCH) Services during the COVID-19 Pandemic in the Context of Odisha, India
Authors: Mishra Bijaya Kumar , Kanungo Srikanta , Patel Kripalini , Swain Swagatika , Dwivedy Subhralaxmi , Panda Subhashree , Karna Sonam , Bhuyan Dinesh , Som Meena , Merta Brajesh Raj , Bhattacharya Debdutta , Kshatri Jaya Singh , Palo Subrata Kumar , Pati Sanghamitra
Published: January 2022

Community health workers (CHW) faced increased challenges in delivering maternal and child health services during the current COVID-19 pandemic. In addition to routine services, they were also engaged in pandemic management. In view of a dearth of evidence, the current study explores the challenges faced by CHWs while rendering maternal and child health services. A qualitative study through in-depth interviews (IDI) and focus group discussions (FGD) in six districts of Odisha was conducted from February to April 2021. Data were analyzed using MAXQDA software. Personal-level challenges, like lack of family support, stress, and fear of contracting COVID-19; facility-level challenges, like transportation problems and inadequate personal protective measures; and community-level challenges, like stigma, resistance, and lack of community support were major hindrances in provisioning routine MCH services. Prevailing myths and misconceptions concerning COVID-19 were factors behind stigma and resistance. Sharing experiences with family, practicing yoga and pranayam, engaging ambulance bikes, financial assistance to mothers, counseling people, and involving community leaders were some effective strategies to address these challenges. Development and implementation of appropriate strategy guidelines for addressing the challenges of frontline warriors will improve their work performance and achieve uninterrupted MCH services during pandemics or similar health emergencies.
GDS: A Genomic Database for Strawberries (Fragaria spp.)
Authors: Zhou Yuhan , Qiao Yushan , Ni Zhiyou , Du Jianke , Xiong Jinsong , Cheng Zongming , Chen Fei
Published: January 2022

Strawberry species (Fragaria spp.) are known as the “queen of fruits” and are cultivated around the world. Over the past few years, eight strawberry genome sequences have been released. The reuse of these large amount of genomic data, and the more large-scale comparative analyses are very challenging to both plant biologists and strawberry breeders. To promote the reuse and exploration of strawberry genomic data and enable extensive analyses using various bioinformatics tools, we have developed the Genome Database for Strawberry (GDS). This platform integrates the genome collection, storage, integration, analysis, and dissemination of large amounts of data for researchers engaged in the study of strawberry. We collected and formatted the eight published strawberry genomes. We constructed the GDS based on Linux, Apache, PHP and MySQL. Different bioinformatic software were integrated. The GDS contains data from eight strawberry species, as well as multiple tools such as BLAST, JBrowse, synteny analysis, and gene search. It has a designed interface and user-friendly tools that perform a variety of query tasks with a few simple operations. In the future, we hope that the GDS will serve as a community resource for the study of strawberries.
Evaluation and Scale Forecast of Underground Space Resources of Historical and Cultural Cities in China
Authors: Shao Jizhong , Liu Guan , Yuan Hong , Song Qize , Yang Minge , Luo Dan , Zhang Xiaosi , Tan Yanran , Zhang Yuxin
Published: January 2022

Following economic growth in the past three decades, rapid urbanization has caused many pronounced issues, such as spatial scarcity and cultural discontinuity, in Chinese historical and cultural cities. In order to better deal with the diversification of underground space resources, data and information, this study introduces a random forest algorithm and proposes a multi-layer information superposition method. According to the characteristics of different information, starting from qualitative and quantitative aspects, we explore the effective performance of the rational development of underground space resources. Taking Yangzhou City, China, as an example, this paper evaluates the suitability and calculates the development volume of urban underground space. The development capacity, potential value, and comprehensive quality of underground space resources are explored in an attempt to demonstrate the practicality and scientificity of the evaluation method for achieving the developmental goals of urban space reconstruction and historic preservation. On this basis, an underground space scale forecast is carried out to provide decision support for relevant planners, managers, and construction personnel that is conducive to the orderly development of urban space, alleviation of increasing human–land conflicts, and coordination of the protection and development of underground space resources in historical and cultural cities, ultimately promoting sustainable development of cities.
Correction: Spenger et al. Glutaric Aciduria Type I Missed by Newborn Screening: Report of Four Cases from Three Families. Int. J. Neonatal Screen. 2021, 7, 32
Authors: Spenger Johannes , Maier Esther M. , Wechselberger Katharina , Bauder Florian , Kocher Melanie , Sperl Wolfgang , Preisel Martin , Schiergens Katharina A. , Konstantopoulou Vassiliki , Röschinger Wulf , Häberle Johannes , Schmitt-Mechelke Thomas , Wortmann Saskia B. , Fingerhut Ralph
Published: January 2022

There was an error in the original publication [...]
Unique Case of Spontaneous Left Main Coronary Dissection in Second Trimester of Pregnancy Successfully Treated with Percutaneous Coronary Intervention: A Happy Ending
Authors: Mantovani Francesca , Navazio Alessandro , Tortorella Giovanni , Guiducci Vincenzo
Published: January 2022

Among pregnant women, SCAD is the most frequent etiology of non-atherosclerotic acute coronary syndrome. SCAD related to pregnancy is more frequent within the first month (especially first week) of puerperium or last trimester, or is otherwise anecdotal. The concomitance of SCAD and pregnancy poses many issues regarding diagnosis and treatment in respect to maternal and fetal safety and requires tailored intervention with close interaction between clinical cardiologists, interventional cardiologists, cardiothoracic surgeons, and obstetricians. We report the case of a patient, pregnant in the second trimester with a life-threatening SCAD, successfully treated with percutaneous coronary intervention with excellent outcome for mother and baby.
Virus-Induced Risk of Hepatocellular Carcinoma: Recent Progress and Future Challenges
Authors: Lupberger Joachim , Baumert Thomas F.
Published: January 2022

Chronic viral hepatitis is a key risk factor for liver fibrosis and hepatocellular carcinoma (HCC) [...]
Surgery and Catheter Ablation for Atrial Fibrillation: History, Current Practice, and Future Directions
Authors: McCarthy Patrick M. , Cox James L. , Kislitsina Olga N. , Kruse Jane , Churyla Andrei , Malaisrie S. Chris , Mehta Christopher K.
Published: January 2022

Atrial fibrillation (AF) is the most common of all cardiac arrhythmias, affecting roughly 1% of the general population in the Western world. The incidence of AF is predicted to double by 2050. Most patients with AF are treated with oral medications and only approximately 4% of AF patients are treated with interventional techniques, including catheter ablation and surgical ablation. The increasing prevalence and the morbidity/mortality associated with AF warrants a more aggressive approach to its treatment. It is the purpose of this invited editorial to describe the past, present, and anticipated future directions of the interventional therapy of AF, and to crystallize the problems that remain.
Fluorescence-Aided Identification Technique (FIT) Improves Tooth Surface Clean-Up after Debonding of Buccal and Lingual Orthodontic Appliances
Authors: Engeler Olivia , Stadler Oliver , Horn Simone , Dettwiler Christian , Connert Thomas , Verna Carlalberta , Kanavakis Georgios
Published: January 2022

The aim of this study was to evaluate the use of fluorescence inducing light to aid the clean-up of tooth surfaces after bracket removal when using buccal or lingual orthodontic appliances. Two full sets of dental arches using extracted human teeth were assembled, with 14 teeth per arch. All teeth were bonded on their buccal and lingual surfaces. After debonding, a single blinded operator performed the tooth surface clean-up, as commonly performed in clinical practice; without the use of fluorescent light (non-FIT) and with two methods using fluorescent light to identify composite remnants on the tooth surfaces (FIT; OPAL and BRACE). Tooth surfaces were scanned before bonding and after clean-up, and the two scans were superimposed using the best-fit method. The results showed that the debonding method, type of tooth and type of tooth surface had a significant effect on the presence of composite remnants, enamel defects, and on debonding time. Contrary to the non-FIT method, there were no composite remnants after clean-up with the use of fluorescence inducing light. Clean-up time was significantly reduced on the buccal surfaces when using the FIT methods. On the lingual surfaces, the FIT methods resulted in larger enamel defects.
An Update on Usage of High-Risk Donors in Liver Transplantation
Authors: Muhammad Haris , Zaffar Duha , Tehreem Aniqa , Ting Peng-Sheng , Simsek Cem , Turan Ilker , Alqahtani Saleh , Saberi Behnam , Gurakar Ahmet
Published: January 2022

The ideal management for end stage liver disease, acute liver failure, and hepatocellular carcinoma (HCC), within specific criteria, is liver transplantation (LT). Over the years, there has been a steady increase in the candidates listed for LT, without a corresponding increase in the donor pool. Therefore, due to organ shortage, it has been substantially difficult to reduce waitlist mortality among patients awaiting LT. Thus, marginal donors such as elderly donors, steatotic donors, split liver, and donors after cardiac death (DCD), which were once not commonly used, are now considered. Furthermore, it is encouraging to see the passing of Acts, such as the HIV Organ Policy Equity (HOPE) Act, enabling further research and development in utilizing HIV grafts. Subsequently, the newer antivirals have aided in successful post-transplant period, especially for hepatitis C positive grafts. However, currently, there is no standardization, and protocols are center specific in the usage of marginal donors. Therefore, studies with longer follow ups are required to standardize its use.
Effect of Early Supraglottic Airway Device Insertion on Chest Compression Fraction during Simulated Out-of-Hospital Cardiac Arrest: Randomised Controlled Trial
Authors: Stuby Loric , Jampen Laurent , Sierro Julien , Bergeron Maxime , Paus Erik , Spichiger Thierry , Suppan Laurent , Thurre David
Published: January 2022

Early insertion of a supraglottic airway (SGA) device could improve chest compression fraction by allowing providers to perform continuous chest compressions or by shortening the interruptions needed to deliver ventilations. SGA devices do not require the same expertise as endotracheal intubation. This study aimed to determine whether the immediate insertion of an i-gel® while providing continuous chest compressions with asynchronous ventilations could generate higher CCFs than the standard 30:2 approach using a face-mask in a simulation of out-of-hospital cardiac arrest. A multicentre, parallel, randomised, superiority, simulation study was carried out. The primary outcome was the difference in CCF during the first two minutes of resuscitation. Overall and per-cycle CCF quality of compressions and ventilations parameters were also compared. Among thirteen teams of two participants, the early insertion of an i-gel® resulted in higher CCFs during the first two minutes (89.0% vs. 83.6%, p = 0.001). Overall and per-cycle CCF were consistently higher in the i-gel® group, even after the 30:2 alternation had been resumed. In the i-gel® group, ventilation parameters were enhanced, but compressions were significantly shallower (4.6 cm vs. 5.2 cm, p = 0.007). This latter issue must be addressed before clinical trials can be considered.
Sclerobanding (Combined Rubber Band Ligation with 3% Polidocanol Foam Sclerotherapy) for the Treatment of Second- and Third-Degree Hemorrhoidal Disease: Feasibility and Short-Term Outcomes
Authors: Pata Francesco , Bracchitta Luigi Maria , D’Ambrosio Giancarlo , Bracchitta Salvatore
Published: January 2022

Background: Sclerobanding is a novel technique combining rubber band ligation with 3% polidocanol foam sclerotherapy for the treatment of hemorrhoidal disease (HD). The aim of this study is to evaluate the feasibility, safety and short-term outcomes of sclerobanding in the treatment of second- and third-degree HD. Methods: A retrospective analysis of second- and third-degree HD cases from November 2017 to August 2021 was performed. Patients on anticoagulants or with other HD degrees were excluded. Follow-up was conducted at 1 month, 3 months, 6 months, 1 year and then every 12 months. Results: 97 patients with second- (20 pts; 20.6%) and third-degree (77 pts; 79.4%) HD with a mean age of 52 years (20–84; SD ± 15.5) were included. Fifty-six patients were men (57.7%) and forty-one women (42.3%). Median follow-up was 13 months (1–26 months). No intraoperative adverse events or drug-related side effects occurred. Minor complications occurred in four patients (4.1%) in the first 30 postoperative days and all resolved after conservative treatment at the 3-month follow-up visit. No mortality or readmissions were observed. Conclusions: Sclerobanding is a safe technique with a low rate of minor postoperative complications. Further studies on larger samples are necessary to establish the effectiveness and long-term outcomes of the technique.
A Systematic Review of the Safety of Blocking the IL-1 System in Human Pregnancy
Authors: Brien Marie-Eve , Gaudreault Virginie , Hughes Katia , Hayes Dexter J. L. , Heazell Alexander E. P. , Girard Sylvie
Published: January 2022

Blockade of the interleukin-1 (IL-1) pathway has been used therapeutically in several inflammatory diseases including arthritis and cryopyrin-associated periodic syndrome (CAPS). These conditions frequently affect women of childbearing age and continued usage of IL-1 specific treatments throughout pregnancy has been reported. IL-1 is involved in pregnancy complications and its blockade could have therapeutic potential. We systematically reviewed all reported cases of IL-1 blockade in human pregnancy to assess safety and perinatal outcomes. We searched several databases to find reports of specific blockade of the IL-1 pathway at any stage of pregnancy, excluding broad spectrum or non-specific anti-inflammatory intervention. Our literature search generated 2439 references of which 22 studies included, following extensive review. From these, 88 different pregnancies were assessed. Most (64.8%) resulted in healthy term deliveries without any obstetrical/neonatal complications. Including pregnancy exposed to Anakinra or Canakinumab, 12 (15.0%) resulted in preterm birth and one stillbirth occurred. Regarding neonatal complications, 2 cases of renal agenesis (2.5%) were observed, and 6 infants were diagnosed with CAPS (7.5%). In conclusion, this systematic review describes that IL-1 blockade during pregnancy is not associated with increased adverse perinatal outcomes, considering that treated women all presented an inflammatory disease associated with elevated risk of pregnancy complications.
Urinary Tract Infections in Kidney Transplant Recipients—Is There a Need for Antibiotic Stewardship?
Authors: Strohaeker Jens , Aschke Victoria , Koenigsrainer Alfred , Nadalin Silvio , Bachmann Robert
Published: January 2022

(1) Background: Urinary tract infections (UTI) are the most common infections after kidney transplantation. Given the risk of urosepsis and the potential threat to the graft, the threshold for treating UTI and asymptomatic bacteriuria with broad spectrum antibiotics is low. Historically fluoroquinolones were prescription favorites for patients that underwent kidney transplantation (KT). After the recent recommendation to avoid them in these patients, however, alternative treatment strategies need to be investigated (2) Methods: We retrospectively analyzed the charts of 207 consecutive adult kidney transplantations that were performed at the department of General, Visceral and Transplantation Surgery of the University Hospital of Tuebingen between January 2015 and August 2020. All charts were screened for the diagnosis and treatment of asymptomatic bacteriuria (ASB) and urinary tract infections (UTI) and the patients’ clinical characteristics and outcomes were evaluated. (3) Results: Of the 207 patients, 68 patients suffered from urinary tract infections. Patients who developed UTI had worse graft function at discharge (p = 0.024) and at the 12 months follow-up (p < 0.001). The most commonly prescribed antibiotics were Ciprofloxacin and Piperacillin/Tazobactam. To both, bacterial resistance was more common in the study cohort than in the control group. (4) Conclusions: Urinary tract infections appear to be linked to worse graft functions. Thus, prevention and treatment should be accompanied by antibiotic stewardship teams.
Two New Sexual Talaromyces Species Discovered in Estuary Soil in China
Authors: Han Pei-Jie , Sun Jian-Qiu , Wang Long
Published: January 2022

In the survey of mycobiota of mudflats in China, two new sexually reproducing Talaromyces sect. Talaromyces species were discovered and studied using a polyphasic approach. These species are named here Talaromyces haitouensis (ex-type AS3.160101T) and Talaromyces zhenhaiensis (ex-type AS3.16102T). Morphologically, T. haitouensis is distinguished by moderate growth, green-yellow gymnothecia, orange-brown mycelium, and echinulate ellipsoidal ascospores. T. zhenhaiensis is characterized by fast growth, absence of sporulation, cream yellow to naphthalene yellow gymnothecia and mycelium, and smooth-walled ellipsoidal ascospores with one equatorial ridge. The two novelties are further confirmed by phylogenetic analyses based on either individual sequences of BenA, CaM, Rpb2, and ITS1-5.8S-ITS2 or the concatenated BenA-CaM-Rpb2 sequences.
Systematic Conservation Planning as a Tool for the Assessment of Protected Areas Network in Jordan
Authors: Boulad Natalia , Al Shogoor Sattam , Sahwan Wahib , Al-Ouran Nedal , Schütt Brigitta
Published: January 2022

The present study aims to use systematic conservation planning to analyse and review the national protected areas (PAs) network in Jordan. The analysis included the application of three modules: the environmental risk surface (ERS), the relative biodiversity index (RBI), and the application of Marxan. The methodology was based on using Marxan to achieve solutions for three scenarios for the PAs network. Marxan was applied to the input data, which included vegetation types, distribution of threatened mammals and plants, locations of currently established PAs and other types of designations. The first two scenarios aimed to conserve 4% and 17%, respectively, of each vegetation type, and 10% and 20%, respectively, of the extent of occurrence of threatened mammals and plants. The third scenario aimed to conserve 17% of each vegetation type and 10% of the extent of occurrence of threatened plants and mammals, except for forest and the Hammada vegetation which had the target of 30% and 4%, respectively. The results of the three scenarios indicated that the boundaries of existing reserves should be extended to achieve the conservation targets. Some currently proposed (PAs), such as the Aqaba Mountains, did not appear in any of the solutions for the three scenarios indicating that the inclusion of these sites in the proposed (PAs) network should be reconsidered. All three scenarios highlighted the importance of having conservation areas between the western and eastern parts of the country. Systematic conservation planning is a structured, replicable, transparent, and defensible method for designing PA networks. It allows for finding efficient solutions building on what is currently conserved and minimizing the fragmentation and cost of the proposed solution for conservation areas.
Inhibition of Non-Small Cell Lung Cancer Proliferation and Survival by Rosemary Extract Is Associated with Activation of ERK and AMPK
Authors: O’Neill Eric J. , Moore Jessy , Song Joon , Tsiani Evangelia Litsa
Published: January 2022

Non-small cell lung cancer (NSCLC) represents an aggressive form of lung cancer which often develops resistance to chemo- and radiotherapy emphasizing a need to identify novel treatment agents to combat it. Many plants contain compounds with anti-inflammatory, antimicrobial, antidiabetic, and anticancer properties and some plant-derived chemicals are used in the treatment of cancer. A limited number of in vitro and in vivo animal studies provide evidence of anticancer effects of rosemary (Rosmarinus officinalis) extract (RE); however, no studies have explored its role in H1299 NSCLC cells, and its underlying mechanism(s) of action are not understood. The current study examined the effects of RE on H1299 cell proliferation, survival, and migration using specific assays. Additionally, immunoblotting was used to investigate the effects of RE treatment on signalling molecules implicated in cell growth and survival. Treatment with RE dose-dependently inhibited H1299 proliferation with an IC50 value of 19 µg/mL. Similarly, RE dose-dependently reduced cell survival, and this reduction correlated with increased levels of cleaved poly (ADP-ribose) polymerase (PARP), a marker of apoptosis. RE was also able to inhibit cell migration as assessed with a wound healing assay. These cellular effects of RE were associated with an increase in phosphorylated levels of extracellular signal-regulated kinase (ERK), AMP-activated protein kinase (AMPK), and its downstream targets ACC, the mTORC1 protein raptor, and decreased p70S6K phosphorylation. More studies are required to fully examine the effects of RE against NSCLC.
Effects of Partial Blackwater Substitution on Soil Potential NI-Trogen Leaching in a Summer Maize Field on the North China Plain
Authors: Zhang Tao , Peng Hao , Yang Bo , Cao Haoyu , Liu Bo , Zheng Xiangqun
Published: January 2022

In China, promoting harmless blackwater treatment and resource utilization in rural areas is a priority of the “toilet revolution”. Exploring the effects of blackwater application in arid areas on soil nitrogen losses can provide a basis for more effective water and fertilizer management. This study analyzed nitrogen leaching and maize yield under blackwater application in the summer maize season of 2020. A total of 5 treatments were used: no fertilizer, single chemical fertilizer application (CF), single blackwater application (HH), and combined chemical fertilizer and blackwater application ratios of 1:1 (CH1) and 2:1 (CH2). The total nitrogen leached from the fertilization treatments was 53.14–60.95 kg·ha−1 and the leached nitrate nitrogen was 34.10–40.62 kg·ha−1. Nitrate nitrogen accounted for 50–62% of the total leached nitrogen. Compared with blackwater treatments, nitrate nitrogen moved into deeper soil layers (80–100 cm depth) during the CF treatment. Compared with CF, HH significantly reduced the maize yield by 24.39%. The nitrogen surplus of HH was higher than that of other fertilizer treatments. Considering nitrogen leaching, maize yield, and economic benefits, the CH2 treatment presented the optimal results. These findings address knowledge gaps and assist in guiding policy-makers to effectively promote China’s “toilet revolution”.
Tribological Behavior of Reinforced PTFE Composites and Un-Reinforced Polyketone-Based Materials against Coated Steel
Authors: Amenta Federica , Bolelli Giovanni , De Lorenzis Stefano , Bertarini Alessandro , Lusvarghi Luca
Published: January 2022

In this study, two polymeric materials were tested in a dry rotating “pin-on-disc” configuration against differently coated surfaces, to evaluate their tribological response under conditions, such as those of rotary lip seals, and to identify the wear mechanism of each coupling. A PTFE based material, reinforced with glass fibers and a solid lubricant, and unreinforced polyketone were tested against a chromium oxide coating deposited by plasma thermal spraying, a CrN/NbN superlattice coating deposited by Physical Vapor Deposition (PVD), and a Diamond-Like Carbon (DLC) coating obtained through a hybrid PVD/PECVD (Plasma-Enhanced Chemical Vapor Deposition) process. The PTFE matrix composite offers better overall performance, in terms of specific wear rates and friction coefficients than polyketone. Although the tribological behavior of this material is generally worse than that of the PTFE matrix composite, it can be used without reinforcing fillers. Our analysis demonstrates the importance of transfer-film formation on the counter-surfaces, which can prevent further wear of the polymer if it adheres well to the counterpart. However, the tribofilm has opposing effects on the friction coefficient for the two materials: its formation leads to lower friction for PTFE and higher friction for polyketone.
Mathematical Modeling and Associated Numerical Simulation of Fusion/Solidification Front Evolution in the Context of Severe Accident of Nuclear Power Engineering
Authors: Drouillet Adrien , Bois Guillaume , Le Tellier Romain , Loubère Raphaël , Peybernes Mathieu
Published: January 2022

Considering transient processes where liquid/solid phase change occurs, this paper focuses on the associated modeling and numerical treatment in the frame of “Computational Fluid Dynamics” simulations. While being of importance in many industrial applications involving solidification and melting of mixed materials, including power and manufacturing engineering, the first application of this work pertains to the analysis of severe accidents in a nuclear reactor. Indeed, in this context, the molten core materials (a.k.a. corium) can form a high-temperature multiphase liquid pool at the boundary of which fusion and solidification phenomena are of prime importance. In this context, even if materials at play are treated as pure components, it is mandatory to distinguish two different phase change temperatures with a solid fusion temperature and a liquid solidification temperature. Accordingly, in the frame of a sharp interface representation, the paper introduces non-classical heterogeneous conditions at the liquid/solid boundary in such a way that both moving interface (through Stefan conditions associated with fusion or solidification) and static interface (imposing heat flux continuity) are supported at the same time on different spatial locations along this boundary. Within a monolithic resolution of Navier–Stokes and heat conduction equations, this interface is explicitly tracked with combined Front-Tracking and VOF methods. In order to ensure zero velocity in the solid phase, an Immersed Boundary Method and a direct forcing penalization are also introduced. The main relevant features of this combination of numerical methods are discussed along with their implementation in the TrioCFD code taking advantage of the pre-existing code capabilities. Numerical simulations including both verification tests and a case of interest for our industrial application are reported and demonstrate the applicability of the proposed triptych model+methods+code to treat such problems. The numerical tools and the simulation code developed in this work could be used not only in the several accident context but also to simulate melting, solidification and fusion processes occurring in aerodynamics, hypersonic reentry vehicles and laser applications to cite but a few.
Bispectral Index Monitoring and Observer Rating Scale Correlate with Dreaming during Propofol Anesthesia for Gastrointestinal Endoscopies
Authors: Matus Helena , Kvolik Slavica , Rakipovic Andreja , Borzan Vladimir
Published: January 2022

Background and objectives: Dreaming is a commonly reported side effect of propofol anesthesia. Materials and Methods: We investigated the inci-dence and character of dreams in patients undergoing intravenous propofol anesthesia and cor-related it with an observer rating scale of facial expression on the seven-point scale from pain to smile. A total of 124 patients undergoing gastrointestinal endoscopy were recruited in the pro-spective observational study. Bispectral index (BIS), blood pressure (BP), and pulse were moni-tored. Upon emergence from anesthesia, the patient’s facial expression was rated numerically. Thereafter, patients were asked whether they had dreams and to rate their dreams as pleasant or unpleasant. The mean age of participants was 53; body mass index, 26.17; duration of procedure, 20 min; and average propofol dose, 265 mg. Results: Dreaming was reported by 43% of patients. Dreams were pleasant in all but one patient. There was a significant correlation of the observer’s rating of facial expression with dreaming (r = 0.260; p = 0.004). Dreamers had higher scores of observer rating of facial expression (1 (0–2) vs. 0.5 (0–1), p = 0.006). Conclusions: BIS values were lower in the dreamers vs. non-dreamers 2 min after the endoscopy started (48 (43–62) vs. 59 (45–71), p = 0.038). Both BIS and observer ratings correlate with dreaming in patients undergoing gastrointestinal endos-copy. Trial registration number: NCT04235894.
Characterization of the Role of Extracellular Vesicles Released from Chicken Tracheal Cells in the Antiviral Responses against Avian Influenza Virus
Authors: O’Dowd Kelsey , Sánchez Laura , Ben Salem Jennifer , Beaudry Francis , Barjesteh Neda
Published: January 2022

During viral respiratory infections, the innate antiviral response engages a complex network of cells and coordinates the secretion of key antiviral factors, such as cytokines, which requires high levels of regulation and communication. Extracellular vesicles (EVs) are particles released from cells that contain an array of biomolecules, including lipids, proteins, and RNAs. The contents of EVs can be influenced by viral infections and may play a role in the regulation of antiviral responses. We hypothesized that the contents of EVs released from chicken tracheal cells are influenced by viral infection and that these EVs regulate the function of other immune cells, such as macrophages. To this end, we characterized the protein profile of EVs during avian influenza virus (AIV) infection and evaluated the impact of EV stimulation on chicken macrophage functions. A total of 140 differentially expressed proteins were identified upon stimulation with various stimuli. These proteins were shown to be involved in immune responses and cell signaling pathways. In addition, we demonstrated that EVs can activate macrophages. These results suggest that EVs play a role in the induction and modulation of antiviral responses during viral respiratory infections in chickens.
An Investigation into Hydraulic Permeability of Fibrous Membranes with Nonwoven Random and Quasi-Parallel Structures
Authors: Liu Zeman , Wang Yiqi , Guo Fei
Published: January 2022

Fibrous membranes with a nonwoven random structure and a quasi-parallel fibrous structure can be fabricated by the electrospinning technique. The membranes with different structures exhibited different behaviors to a hydraulic flow passing through the membranes. This work presents the effects of the fiber arrangement, fiber diameter, and deformations of the fibers on the hydraulic permeability. The results showed that the hydraulic flow can generate an extrusion pressure which affects the porosity and pore structure of the fibrous membranes. The quasi-parallel fibrous membranes and nonwoven membranes exhibited similar variation tendencies to the change of the experimental variables. However, the quasi-parallel fibrous membranes exhibited a higher sensibility to the change of the hydraulic flow rate. The hydraulic permeability of the quasi-parallel fibrous membranes was further analyzed with packing state models in this work.
Improving Isolation of Extracellular Vesicles by Utilizing Nanomaterials
Authors: Zhang Haiyang , Zhang Qi , Deng Yuanyuan , Chen Mengxi , Yang Chenxi
Published: January 2022

Extracellular vesicles (EVs) as the new form of cellular communication have been demonstrated their potential use for disease diagnosis, prognosis and treatment. EVs are vesicles with a lipid bilayer and are present in various biofluids, such as blood, saliva and urine. Therefore, EVs have emerged as one of the most appealing sources for the discovery of clinical biomarkers. However, isolation of the target EVs from different biofluids is required for the use of EVs as diagnostic and therapeutic entities in clinical settings. Owing to their unique properties and versatile functionalities, nanomaterials have been widely investigated for EV isolation with the aim to provide rapid, simple, and efficient EV enrichment. Herein, this review presents the progress of nanomaterial-based isolations for EVs over the past five years (from 2017 to 2021) and discusses the use of nanomaterials for EV isolations based on the underlying mechanism in order to offer insights into the design of nanomaterials for EV isolations.
Extracorporeal Hyperoxygenation Therapy (EHT) for Carbon Monoxide Poisoning: In-Vitro Proof of Principle
Authors: Steuer Niklas B. , Schlanstein Peter C. , Hannig Anke , Sibirtsev Stephan , Jupke Andreas , Schmitz-Rode Thomas , Kopp Rüdger , Steinseifer Ulrich , Wagner Georg , Arens Jutta
Published: January 2022

Carbon monoxide (CO) poisoning is the leading cause of poisoning-related deaths globally. The currently available therapy options are normobaric oxygen (NBO) and hyperbaric oxygen (HBO). While NBO lacks in efficacy, HBO is not available in all areas and countries. We present a novel method, extracorporeal hyperoxygenation therapy (EHT), for the treatment of CO poisoning that eliminates the CO by treating blood extracorporeally at elevated oxygen partial pressure. In this study, we proof the principle of the method in vitro using procine blood: Firstly, we investigated the difference in the CO elimination of a hollow fibre membrane oxygenator and a specifically designed batch oxygenator based on the bubble oxygenator principle at elevated pressures (1, 3 bar). Secondly, the batch oxygenator was redesigned and tested for a broader range of pressures (1, 3, 5, 7 bar) and temperatures (23, 30, 37 °C). So far, the shortest measured carboxyhemoglobin half-life in the blood was 21.32 min. In conclusion, EHT has the potential to provide an easily available and effective method for the treatment of CO poisoning.
High-Efficiency Separation of Mg2+/Sr2+ through a NF Membrane under Electric Field
Authors: Liu Huan , Li Quan , He Benqiao , Sun Zhengguang , Yan Feng , Cui Zhenyu , Li Jianxin
Published: January 2022

The efficient separation of Sr2+/Mg2+ through nanofiltration (NF) technology is a great challenge because Sr2+ and Mg2+ ions are congeners with the same valence and chemical properties. In this work, an NF membrane under an electric field (EF) was successfully employed to separate Mg2+ and Sr2+ ions for the first time. The effects of current densities, Mg2+/Sr2+ mass ratios, pH of the feed, and coexisting cations on separation performance were investigated. Dehydration of Sr2+ or Mg2+ ions under EF was proved by molecular dynamics simulation. The results showed that a high-efficient separation of Mg2+/Sr2+ was achieved: Mg2+ removal of above 99% and increase in Sr2+ permeation with increasing EF. A separation factor reached 928 under optimal conditions, far higher than that without EF. The efficient separation of Mg2+/Sr2+ ions was mainly due to rejection of most Mg2+ ions by NF membrane and in situ precipitation of partly permeated Mg2+ ions by OH− generated on the cathode under EF. Meanwhile, preferential dehydration of Sr2+ ions under EF due to lower hydration energy of Sr2+ compared with Mg2+ resulted in an increase of permeation of Sr2+ ions. This work provided a new idea for separation of congener ions with similar valence and chemical properties.