bluuebunny/crossref_metadata_2025 huggingface dataset:
Preliminary study of Toxocara canis Recombinant C- type Lectin as a suitable antigen for serodiagnosis of human toxocariasis
Authors: Malekzade Parmida , Hosseini Seyed Hossein , Shahbakhsh Mahsa , Shayan Parviz , Zibaei Mohammad , Jamshidi Shahram , Rismani Elham , Moghadasi Abdorreza Naser , Akrami Mohammad , Jalousian Fateme
Published: August 2022

C-type lectin (CTL) is the main protein part of the secretory-excretory product, secreted by secondary stage Toxocara canis larvae. Considering the antigenic characteristics of the protein, overlapping regions were identified as the highly potent MHC-I and MHC-II binding epitopes. Furthermore, several distinct regions were distinguished as the linear and non-linear B cell epitopes. To use this protein as a candidate for serological diagnosis, the recombinant pET-32a(+) plasmid containing the 660 bp sequence of Toxocara canis C-type lectin gene was synthesized and successfully cloned and expressed in Escherichia coli (DE3+). Subsequently, the expression of the insert was confirmed by SDS-PAGE and Western blot using an anti-His monoclonal antibody. Fifty-six serum samples were collected from pet owners. Forty-four samples were negative and twelve samples were positive performed with a commercial ELISA kit. The same samples were also tested using dot blot and western blot analysis prepared with recombinant CTL antigen (rCTL). Interestingly, the results achieved by dot blot and western blot analysis generated with rCTL showed that two negative samples determined by ELISA tests were positive in the mentioned tests, The other samples had comparable results as well as the ELISA test. A comparison of the test results of 56 human sera showed that the results were the same between the three test methods and the Kappa coefficient was calculated 1(p-value=7.25) and 0.9(p-value=1.29). It was suspected that the rCTL is more sensitive than the whole antigen secreted by Toxocara canis. In conclusion, this antigen could be further evaluated within the supplementary studies to improve the toxocariasis diagnostic kits.
Evaluating the Risk of Thermal Damage to Dermal Tissue in Cataract Surgery Related Phacoemulsification
Authors: Ramprasad Aarya , Qureshi Fahad , White William Abraham
Published: August 2022

BackgroundModern cataract surgery relies on a phacoemulsification handpiece which can generate excessive heat leading to corneal burns. Burns outside of the eye are not well documented. This study aimed to determine whether the handpiece could cause thermal injury to patients outside the eye and if the device is able to ignite drapes.Methods3 trials using porcine skin were completed to assess the possibility and speed of char occurring with the handpiece tip being in contact with different materials including the tip directly on a pig foot with no drape, a Henry Schein standard drape, and a 3M plastic Steri-Drape. Trials were performed with the silicone cover on the tip and without. Each trial was repeated 3 times with no statistically significant difference between trials of the same material (P < 0.05). The Infiniti Vision System by Alcon Labs was used.ResultsWith no drape, the tip without a sleeve caused char within 3.0 seconds while with a sleeve caused char at 10.0 seconds or above (Fig. 1). Trials with the standard drape did not cause char but did burn a hole through the drape regardless of the sleeve. The tip with the sleeve burnt through the plastic drape, charring in 2.7 seconds, while no sleeve led to the same result in less than 1.0 seconds (Fig. 2). In all trials, charring was quicker without the silicone sleeve. No trials resulted in drape ignition.ConclusionsCharred skin is considered to be a 3rd-degree full-thickness burn with less severe burns possibly not being visible. One trial showed the body of the handpiece at 53.6℃ degrees with immediate destruction of the epidermis known to occur above 44℃. The silicone tip proved to be protective but not effective in preventing burns. The trial was not completed in an oxygen-enriched environment. Focused efforts on using the silicon tip, irrigation, and placing the phacoemulsification handpiece on a surface other than the patient could prevent burns from occurring.
The complete chloroplast genome of Punica granatum var. pleniflora
Authors: Zhu Jiamin , Yang Xia , Zhao Yuxue , Wu Liu-Yan
Published: August 2022

In this study, the complete chloroplast (cp) genomes of Punica granatum var. pleniflora were determined. The full-length P. granatum var.pleniflora cp genome was 158,655 bp, including a large single-copy(LSC) region of 89,037 bp, a small single-copy(SSC) region of 18,686 bp, and a pair of inverted repeats(IR) of 25,466 bp. A total of 112 genes, including 78 protein-coding genes, 30 tRNA genes, and 4 rRNA genes were predicted by the analyses. Furthermore, the overall GC content of the complete cp genome was 36.90%. Phylogenetic analysis further suggested that P. granatum var. pleniflora was closely related to Punica granatum Linn, with high bootstrap values.
Gap-filling of ocean color over the tropical Indian Ocean using Monte-Carlo method
Authors: Modi Aditi , K Roxy M , Ghosh Subimal
Published: August 2022

Continuous remote-sensed daily fields of ocean color now span over two decades; however, it still remains a challenge to examine the ocean ecosystem processes, e.g., phenology, at temporal frequencies of less than a month. This is due to the presence of significantly large gaps in satellite data caused by clouds, sun-glint, and hardware failure; thus, making gap-filling a prerequisite. Commonly used techniques of gap-filling are limited to single value imputation, thus ignoring the error estimates. Though convenient for datasets with fewer missing pixels, these techniques introduce potential biases in datasets having a higher percentage of gaps, such as in the tropical Indian Ocean during the summer monsoon, the satellite coverage is reduced up to 40% due to the seasonally varying cloud cover. In this study, we fill the missing values in the tropical Indian Ocean with a set of plausible values (here, 10,000) using the classical Monte-Carlo method and prepare 10,000 gap-filled datasets of ocean color. Using the Monte-Carlo method for gap-filling provides the advantage to estimate the phenological indicators with an uncertainty range, to indicate the likelihood of estimates. Quantification of uncertainty arising due to missing values is critical to address the importance of underlying datasets and hence, motivating future observations.
Andrographolide Derivative AL-1 Ameliorates LPS-induced Acute Lung Injury by
Inhibiting NLRP3 Inflammasome and Lung Permeability
Authors: Li Tangjia , Zhang Chu , Wei Yuke , Zhong Haijing , Shan Luchen , Yu Pei , Wang Yuqiang , Xu Lipeng
Published: August 2022

Background:

Acute lung injury (ALI) is a serious respiratory disease with a high mortality rate, and
there is an urgent need for a more effective treatment strategy. Andrographolide derivative AL-1 has been identified
to possess anti-inflammatory activity. However, whether it could reduce LPS-induced lung injury in mice
through inhibiting NLRP3 inflammasome activation and protecting lung permeability has not yet been elucidated.
In the present research, we investigated the protective effect of AL-1 on ALI mice and demonstrated the
potential mechanisms.

Methods:

Male Balb/c mice were anesthetized with isoflurane, and ALI mice were induced by intratracheal
instillation of LPS. The mice were euthanized after LPS administration for 12 h, then bronchoalveolar lavage
fluid (BALF) and lung tissues were collected. The levels of inflammatory factors were measured by ELISA
assay, and HE staining and lung injury scoring were used to evaluate the pathological changes in the pulmonary
tissues. Immunohistochemistry and immunofluorescence examination were conducted to detect the expression
levels of related proteins. Western blot was performed to measure the levels of NLRP3 inflammasome and tight
junction proteins.

Results:

The study indicated that AL-1 effectively alleviated lung injury by reduction of proinflammatory cytokine
levels, MPO activity, lung W/D ratio, and total protein levels. Furthermore, AL-1 improved pathological
changes in lung tissue and significantly reduced the infiltration of inflammatory cells. Administration with
AL-1 markedly inhibited the expression of NLRP3, ASC, Caspase-1, IL-1β, gasdermin D (GSDMD), and
VCAM-1 but increased the expression of ZO-1, Occludin, JAM-A, and Claudin-1.

Conclusion:

Taken together, these results demonstrated that AL-1 ameliorated pulmonary damage by inhibiting
the activation of the NLRP3 inflammasome pathway and restoring TJ protein expression.
Application of Therapeutic Nanoplatforms as a Potential Candidate for the Treatment
of CNS Disorders: Challenges and Possibilities
Authors: Sa Pratikshya , Singh Priya , Dilnawaz Fahima , Sahoo Sanjeeb Kumar
Published: August 2022

Drug delivery to central nervous system (CNS) diseases is one of the most challenging tasks. The
innate blood-brain barrier (BBB) and the blood-cerebrospinal fluid (BCSF) barrier create an obstacle to effective
systemic drug delivery to the CNS, by limiting the access of drugs to the brain. Nanotechnology-based drug
delivery platform offers a potential therapeutic approach for the treatment of neurological disorders. Several
studies have shown that nanomaterials have great potential to be used for the treatment of CNS diseases. The
nanocarriers have simplified the targeted delivery of therapeutics into the brain by surpassing the BBB and actively
inhibiting the disease progression of CNS disorders. The review is an overview of the recent developments
in nanotechnology-based drug delivery approaches for major CNS diseases like Alzheimer's disease,
Parkinson's disease, ischemic stroke, and Glioblastoma. This review discusses the disease biology of major
CNS disorders describing various nanotechnology-based approaches to overcome the challenges associated
with CNS drug delivery, focussing on nanocarriers in preclinical and clinical studies for the same. The review
also sheds light on the challenges during clinical translation of nanomedicine from bench to bedside. Conventional
therapeutic agents used for the treatment of CNS disorders are inadequate due to their inability to cross
BBB or BCSF, higher efflux from BBB, related toxicity, and poor pharmacokinetics. The amalgamation of
nanotechnology with conventional therapeutic agents can greatly ameliorate the pharmacokinetic problems and
at the same time assist in efficient delivery to the CNS.
Linkages Between Micronutrients and Health
Authors: Chiplonkar Shashi A.
Published: August 2022

Micronutrients perform a key role in almost all vital body functions like
those related to vision, immunity, bone formation, brain function and many others that
help in proper growth and maintaining good health. Deficiency of any one or more of
the micronutrients can have a negative impact on the overall health. The causes of
micronutrient deficiencies and their signs and symptoms are explained in this chapter
in a precise manner. Plant and animal food sources with high micronutrient contents
with ready-to-eat food supplements per serving size are illustrated for each
micronutrient. These can serve as a guideline for preventive measures and diet therapy.
Micronutrients are increasingly used as an adjunct therapy in managing various health
problems. Effective therapeutic uses of vitamins and minerals are exemplified for a
ready reference. Overdose of synthetic supplements of micronutrients and certain
environmental conditions may result in toxic effects. To address this, the upper limit
for micronutrient intakes and toxic effects of excess micronutrient intakes are also
described. The relevance of the yogic concept of ‘sattvic’ diet in modern nutrition and
the potential of yoga for micronutrient absorption is also highlighted. The approach of
yoga towards healthy diet and the relation between diet and psychological health is
explained based on experimental data. The importance of specific micronutrients in
health at different stages of life, from pregnancy to old age, is elucidated and remedial
measures are suggested. Lastly, evidence for micronutrient supplements as an adjunct
therapy for COVID-19 is briefly described. 
Micronutrients: The Key to Good Health
Authors: Chiplonkar Shashi A.
Published: August 2022

Micronutrients, which comprise minerals and vitamins, are essential for a variety of biological processes such as growth, immune response, regulation of gene expression and cognitive functions. Micronutrients: The Key to Good Health presents key information about the causes, signs and symptoms of micronutrient deficiencies along with remedial dietary measures. The book also provides authentic knowledge of the nature, structure and metabolic functions of vitamins and essential minerals in a reader-friendly manner. The aim of the book is to provide comprehensive knowledge about micronutrients and their role in a balanced diet for maintaining personal health.

Key features:

- Covers the basics of nutrition and the importance of micronutrients in health over 5 reader-friendly chapters

- Covers metabolic functions of micronutrients

- Covers the development of micronutrient rich food products

- Includes information about micronutrient deficiencies with remedial dietary measures in terms of ready-to-eat food supplements in a precise manner

- Includes information about phytochemical nutrients, prebiotics and postbiotics

- Informs the reader about myths on micronutrients

- Includes several informative illustrations, tables and appendices on daily nutrient requirements, diet assessment and composition and much more,

This book serves as a ready reference for the health science students, public health workers, nutrition professionals, and health-conscious readers.
O que há de poesia no animal: uma análise da “prosa em prosa” de Jean-Marie Gleize
Authors: Villon Renata Coutinho
Published: August 2022

This article seeks to introduce the work of the contemporary French poet Jean-Marie Gleize, starting with theoretical assumptions that he himself proposes based on his studies of French poetry, with a focus on modern poetry. It is then analyzed what will lead him to advocate the writing of what he named “prose in prose,” based on an observation, mainly, of the Baudelairian work and the change that seemed to take place in the poet’s writing between his first book of poems, Les fleurs du mal (2016), and the second, Le spleen de Paris (2007). Making also an analysis of the figures of dogs and cats that appear differently in each of these works, Gleize proposes a vision of the “dog” linked to this “prosified” poetry, more connected to the city and to reality itself, which is the writing he defends in the face of modern life. The article will also make propositions, therefore, about an “animalistic” writing that seems to have been suggested by other writers, based on the analysis of animal studies.
Observações sobre as disciplinas em temática sobre Gestão de Documentos dos cursos de Arquivologia no Brasil
Authors: Gonçalves Eliane Silveira , Maia Hemmilys Karolinne de Sousa , Perdigão Kethlen Anne Araújo
Published: August 2022

University programs are the way to develop knowledge and improvescientific research, as well as the locusof knowledge to be applied in professional practice. Records Management in the theoretical field of Archival Science has become one of the main supports for document organization, facilitating the preservation of produced documents, rationalizing their storage, and making access to archival information more efficient. In this perspective, the objective was to observe how the theme of Records Management is presented in university programs in Archival Science in Brazil. Research was carried out with a basic nature, a quali-quantitative approach, with emphasis on quantitative data collected inthe Political Projects of Programs, having the support of the documental and bibliographic research method to understand how Records Management and its theoretical and practical approaches are being taught in university programs. Based on the data collected from the 16 Archival Science programs in Brazil,located in public universities, 51 compulsory subjects that cover the theme were identified in the PoliticalProjectsof Programs, drawinga total of 987 bibliographic references amongthose considered basic and complementary.
FARKLI KOŞULLARDAKİ KAYIP VERİ ORANININ İÇ TUTARLIĞA ETKİSİ
Authors: BAYHAN Ayşe , DOĞAN Nuri , SOYSAL Sümeyra
Published: August 2022

Bu çalışmada farklı oranlarda kayıp veri içeren veri setleri için hesaplanan Cronbach α değerindeki değişim örneklem büyüklüğü (100, 250, 500, 1000), test uzunluğu (5,10, 25, 50), dağılım biçimi (normal, sağa çarpık, sola çarpık) ve puanlama türü (Likert, ikili) değişkenleri açısından incelenmiştir. Çalışmada kullanılan tam veri setleri Wingen 3 programında belirlenen koşullar altında 20’li tekrarlar hâlinde üretilmiş tam veri setlerinin her biri R programında yazılan kodlarla rassal olarak kayıp veri mekanizmasına uygun olarak %5, %10 ve %20 oranında değer silinerek Cronbach α güvenirlik değerinin hesaplanacağı eksik veri setleri elde edilmiştir. Bulgulara göre farklı puanlama türleri için örneklem büyüklüğü arttıkça Cronbach α değerindeki değişimin azaldığı görülmüştür. Benzer şekilde madde sayısı arttıkça Cronbach α değerindeki değişim azalmaktadır. Farklı oranlarda kayıp veri içeren veri setleri için hesaplanan Cronbach α değerindeki değişimin dağılım biçimine (normal dağılım, sağa çarpık dağılım, sola çarpık dağılım) bağlı olmadığı ortaya konmuştur.
MESLEKİ REHBERLİK VE DANIŞMA UYGULAMALARI DERSİNE İLİŞKİN PSİKOLOJİK DANIŞMAN ADAYLARININ VE ÖĞRETİM ELEMANLARININ UYGULAMA VE SÜPERVİZYON DENEYİMLERİ
Authors: ERKAN ATİK Zeynep , ULAŞ KILIÇ Özlem , ZORBAZ Selen
Published: August 2022

Rehberlik ve Psikolojik Danışma programlarında lisans öğrencilerinin süpervizyon altında uygulama yaptıkları derslerden biri de Mesleki Rehberlik ve Psikolojik Danışma Uygulamaları dersidir. Bu ders kapsamında kariyer danışması ve rehberliği hizmetlerini sunmaya ilişkin yeterlikler kazandırılmaya çalışılmaktadır. Bu nedenle söz konusu ders sürecinde öğretim elemanlarının sunduğu uygulama fırsatları ve süpervizyon hizmetleri kariyer psikolojik danışmanı yetiştirilmesinde önemli bir yer kaplamaktadır. Bu çalışmanın amacı da lisans eğitiminde MRDU dersi kapsamındaki uygulama ve süpervizyon deneyimlerinin niceliği ve niteliğinin süpervizör ve öğrenci görüşlerine dayalı olarak incelenmesidir. Çalışma nitel yaklaşıma dayalı olarak gerçekleştirilmiş ve açık ve kapalı uçlu sorular bir arada kullanılmıştır. Çalışmaya 39 farklı üniversiteden MRDU ders sorumlusu olan 56 öğretim elemanı ve 23 farklı üniversiteden MRDU dersini almış 223’ü kadın 55’i erkek toplam 278 RPD lisans programı öğrencisi katılmıştır. Çalışmada araştırmacılar tarafından oluşturulan iki farklı form kullanılmış ve formlar çevrimiçi ortamda katılımcılara uygulanmıştır. Elde edilen bulguların uygulama sürecinin yapılandırılması, süpervizyon sürecinin yapılandırılması ve süreçlerin yapılandırılmasında güçlükler olmak üzere üç kategoride toplandığı görülmüştür. Bulgular literatüre dayalı olarak tartışılmış ve öneriler sunulmuştur.
Compatibility of Neem (Azadirachta indica) and Beauveria bassiana for control of Spodoptera exigua and the Theoritical Impact to The Agroecorsystem
Authors: Sih Kurnianto Agung , Prastiwi Santi , Dewi Nilasari , Ilham Tanzil Ahmad , Muhlison Wildan , Wagiyana Wagiyana
Published: August 2022

Spodoptera exigua (Hubner) (Lepidoptera: Noctuidae) is the biggest threat to onion farming, especially in the vegetative phase. In severe attacks, this pest can cause yield losses of up to 100%. Compatibility is one way to increase the efficiency and effectiveness of pest control by using plant-based pesticides or biological agents. Neem Leaf Extract (Azadirachta indica, acronym=NLE) has an azadirachtin compound that can inhibit insect growth, reduce appetite, reproduction, and hatch eggs. The fungus Beauveria bassiana (acronym=Bb) can secrete chitinase, lipase, proteinase enzymes that are able to decompose insect cuticles. NLE was obtained through the extraction method of plant-based pesticides and stored at 4oC until the experiment time. The Bb used was a commercial B. bassiana inoculum in the flour form with a density of 4.5 x 1010 spores/gram (trade name = Natural BVR). To determine the advantages of compatibility, this study is held by a single toxicity test of Bb and NLE, and the combined toxicity test of both. This study used a completely randomized design (CRD) consisting of 6 treatments with 4 replications, each replication used 12 larvae (a total of 48 larvae). Combination toxicity has a higher toxicity than the single use of NLE and Bb. At the LC 95 level, the combination treatment of Bb and NLE was 1.06-1.15 times more toxic than the single treatment of Bb, and 6.87-7.79 times more toxic than the single treatment of NLE. NLE and Bb are considered to have high compatibility (strong synergistic with GI value <0.5). Theoretically, the compatibility of NLE plant-based pesticides and Bb is very promising to replace chemical pesticides that have long-term adverse effects on agroecosystems
Analysis The Effect Of Work Motivation And Competence On Employee Performance (Study On Employee’s PT JCI Padang, West Sumatera)
Authors: Choirunnisa Yusti , Koestiono Djoko
Published: August 2022

This research was conducted at PT Japfa Comfeed Indonesia Unit Padang, which is an animal feed processing company the biggest and leading in the country. The study aims to describe and analyze the effect of work motivation and competence on employee performance at PT JCI Padang.The approach to this research uses probability sampling with simple random sampling technique. The sample used in this study was staff at the junior management level which numbered 47 people. This research method uses SEM-PLS (Structural Equation Model-Partial Least Square) to see how much influence exogenous variables have on endogenous variables using WarpPLS 6.0 software. The results of this study indicate that: (1) Work motivation has a positive and highly significant effect on employee performance of 0.556, (2) Competence has a positive and highly significant effect on employee performance of 0.333. The lowest indicator value on work motivation and competence is the need for existence and attitude. Therefore the company is expected to review the salary issues received by employees and the company should provide training about problem solving solutions.
Sustainable Sustainable Livelihood Asset-Based Strategy for Rosella Farmers in Pagung Village, Semen District, Kediri Regency
Authors: Putra Demmy , Setiawan Budi , Andriani Dwi
Published: August 2022

Rosella commodity prices, in the range of 15,000 to 30,000, are still relatively low and unstable. Therefore, it is a factor of low motivation in rosella farming. On the other hand, rosella as an annual crop with one big harvest certainly has an impact on farmers' income. The research approach used is a quantitative approach. In this study, livelihood strategies are based on asset characteristics that can be accessed by farmers using 43 respondents and livelihood vulnerability index (LVI) analysis and descriptive analysis. Based on the findings, it showed that the characteristics of the asset that is the lowest accessed and the highest level of vulnerability by rosella farmers is social Asset due to the dependence on meeting their daily needs from government assistance. The results of the study showed that the consolidation strategy was the most appropriate strategy in the context of sustainable livelihoods for rosella farmers through the use of yards to raise cattle or goats. The choice of strategy is based on the lack of access to social Asset for farmers' livelihoods. Where, by raising livestock, farmers can earn additional income outside of rosella farming.
AFFECTIVE BEHAVIORAL THERAPY (ABT): FUNDAMENTAL PRACTICE IN THERAPEUTIC LISTENING AND OCCUPATIONAL THERAPY FOR PRIMARY CARE WORKERS DURING THE COVID-19 PANDEMIC
Authors: Blujus Dos Santos Rohde Ciro , Fernando Da Silva Márcio , Tavares Hermano
Published: August 2022

in various communities. Primary healthcare workers (PHCW), are at the frontline of COVID-19 crisis, having to deal with infected patients and their families. Objective: To evaluate the efficacy of Affective Behavioral Therapy (ABT) for the treatment and prevention of mental health disorders for PHCW. Methods: PHCW from a primary care unit (PCU) were invited to participate in an ABT group. They answered the Patient Health Questionnaire-4 (PHQ-4) about how they felt before the sessions and after them. Results: 10 sessions were offered between April and June 2020 and 30 PHCW participated, 22 women and 08 men mean age was 36.8 (SD=9.7) years old. The average number of participants per session was 4.4 (SD=2.2). Pre- and post-intervention PHQ-4 scores showed significant improvement in total score (p < 0.001). Conclusion: PHCWs mental health is strategic for the management of the community during crises related to natural crises. The ABT was effective in supporting the mental health of PHCW during the COVID-19 epidemic. Future controlled studies must be conducted in order to further test its efficacy. Keywords: COVID-19, mental health, primary care, therapeutic listening, occupational therapy, affective behavioral therapy
COMBINATION OF PATHOLOGY OF THE BRAIN AND THE OPTIC NERVE
Authors: Safonenko Alexandra , Ioyleva Elena , Kabanova Evgeniya , Seregina Tatiana
Published: August 2022

The relevance of assessing the lesion of the visual analyzer with optic disc drusen and the possibility of identifying concomitant pathologies, increases with the introduction of the latest diagnostic techniques.In the S. Fyodorov Eye Microsurgery Federal State Institution, Moscow, a patient, 50 years old, complaining of episodic blurred vision. The diagnosis revealed the drusen of the optic nerve head and concomitant congenital neurological pathology. The patient was consulted by a neurosurgeon with the aim of delivering a diagnosis and determining treatment tactics. The diagnosis was made: Arteriovenous malformation of the deep arteries of the sections of the left parietal lobe. After assessing the size of the arteriovenous malformation, its location in the functionally important area of the brain, it was decided to follow up the patient in dynamics
KABUKI SYNDROME AND EPILEPSY
Authors: Zhitomirskaya Marina , Treskina Galina , Dengina Nina , Odinstova Galina
Published: August 2022

Background: Kabuki syndrome is a rare disease. In 2018, a global consensus on diagnostic criteria for Kabuki syndrome (KS) was published, diagnosing KS both with and without molecular genetic confirmation. Neurological symptoms are a major clinical problem in more than 80% of cases. Seizures occur with a frequency of 10% to 40% in Kabuki syndrome. Various degrees of severity of epilepsy in KS have been reported. There are isolated cases of West syndrome in KS worldwide. Objective: To investigate the frequency of epilepsy in KS and the features of West syndrome comorbid with KS, using evidence from reviews and describing the clinical presentation based on the history of a patient with molecularly positive KS. Methods: The study design was a two-centre retrospective observational study. Study subjects were paediatric patients with genetically verified KS. We conducted our own observation of 4 patients with KS and detailed a clinical case of a male patient with West syndrome in KS. Results: Epilepsy was found in 2 patients. Onset of epilepsy at 1.5 and 20 months of age. Both cases were characterised by a severe course of epilepsy. A rare form of epilepsy in KS, West syndrome, was described in patient M. Initial complaints included first seizures and delay in psychomotor development from 1.5 months of age. The clinical features of West syndrome, an electroencephalographic image, MRI of the brain, and the patient's seizure management were presented. Conclusion: Epilepsy in KS is not an uncommon clinical symptom that exacerbates the underlying disease. KS in combination with West syndrome exacerbates neurological deficits and leads to a significant delay in psycholinguistic and psychomotor development against a background of low efficacy of medication and differential effects of hormones and ketogenic diet.
PROFILE OF CHARACTERISTIC, RISK FACTOR, AND STROKE SEVERITY ON INFARCTION STROKE PATIENTS
Authors: Rizki Ramadhani Aisyah , Saiful Ardhi Mohammad , Prajitno Subur
Published: August 2022

Background: Stroke is the second leading cause of death and the third leading cause of morbidity. Therefore, it is important to know which risk factor that most patients have. Stroke can be divided according to its severity using NIHSS, NIHSS itself has strong ability to predict outcomes after stroke Objective: This study’s aim to learn about profile of characteristic, risk factor, and stroke severity on infarction stroke patients in ward Seruni A RSUD Dr. Soetomo from July 2018 – June 2019. Methods: This descriptive observational study’s samples are patients with infarction stroke in ward Seruni A RSUD Dr. Soetomo from July 2018 – June 2019. The observed profile include age, sex, ethnic, family history of vascular disease, history of hypertension, diabetes, heart disease, dyslipidemia, obesity, smoking, alcohol consumption, physical inactivity, and stroke severity. Results: From 200 patiens, 55% (110) are 56-70 years old. 61% (122) patients are male. 81 out of 96 are Javanese. 25 out of 135 have family history of vascular disease, 140 out of 194 have hypertension, 69 out of 190 have diabetes, 27 out of 183 have heart disease, 101 out of 174 have dyslipidemia, 62 out of 162 have obesity, 33 out of 82 smoke, 4 out of 63 consume alcohol, and 50 out of 55 are inactive physically. On admission, out of 60 patients, 27 have moderate stroke, 25 minor, and 8 moderate to severe. On discharge, out of 49 patients, 23 have moderate stroke, 20 minor, 3 moderate to severe, and 3 severe. Conclusion: Infarction stroke patients were mostly male and the peak incidence occur between 56-70 years old. Most patients have histories of hypertension, dyslipidemia, and diabetes. On admission and discharge, most patients have moderate stroke, followed by mild stroke.
NEUROPROTECTIVE EFFECT OF CINNAMON ACTIVE COMPOUNDS VIA ACTIVATION OF SIRT1: A MOLECULAR DOCKING APPROACH
Authors: Kalsum Umi , Khotimah Husnul , Titisari Sulihah Nurfaizah , Firdaus Theakirana , Aulia Lisabilla Fitrah , Fukata Editya , Kurnia Permatasari Happy , Andarini Sri
Published: August 2022

Background: Neurodegenerative diseases are the main cause of morbidity and disability in the elderly. SIRT1 activation has been gaining popularity as novel treatment target. Cinnamon is known to possess neuroprotective abilities, however the mechanism in which it protects the brain is still limited. Objective: This research aimed to determine the interaction between several cinnamon active compounds with SIRT1 Methods: We used in-silico method to determine the molecular interactions between cinnamon main compounds as the ligands to target protein SIRT1. SIRT1 3D structure was retrieved from the Protein Data Bank and 4 ligands (Cinnamaldehyde, Caffeic Acid, Epicatechin, and Trigonelline) structures were obtained from PubChem web server, and we used Resveratrol as positive control ligand. SwissADME, Pyrx, Pymol, and Biovia Discovery Studio software were utilized in this research Results: All four ligands fulfilled Lipinski Rule of 5 criteria therefore they are suitable for oral administration. It was discovered in this study that epicathecin had higher binding affinity than the control ligand Resveratrol and interacted with SIRT1 in the similar amino acid residue as Resveratrol did. The binding pocket interaction between all ligands and SIRT1 are the same. Conclusion: Epicathecin, as one of the main cinnamon compounds, may possess neuroprotective properties by interacting with SIRT1. We pproposed that further research be implemented to investigate epicathecin biological effects on SIRT1 in vitro or in vivo.
Livestock farmers’ perception, perceived impacts, and adaptations to climate change in Koinadugu district, Sierra Leone
Authors: Sesay Abdul Rahman , Sesay Abdul Rahman , Kallon Sanpha
Published: August 2022

Climate change is having a negative influence on agriculture and livestock production systems. This study aims to assess livestock farmers’ perceptions and adaptive responses to climate change in the Koinadugu area of northern Sierra Leone. A sample of 126 livestock farmers was selected from four chiefdoms. The data were collected using a semi-structured questionnaire and meteorological time series data. The Statistical Package for Social Sciences (SPSS) version 23.0 and excel were used to analyze the data. According to the respondents’ socioeco­nomic characteristics, most farmers (93.7%) were males between 46 and 55 years. The majority of the farmers (58.7%) were married, and family sizes ranged from 5-8 individuals per household. Most of the livestock farmers (59.5%) in the study area had no formal education. Farmers attested to their awareness of climate change by essential indications such as increasing heat waves (77.0%), decreased rainfall (54.0%), and an increase in humidity (82.5%). Feed shortages (x̄ = 4.14), water scarcity (x̄ = 4.10), and higher veterinary care expenditures (x̄ = 4.25) are some of the impacts of climate change on livestock production. Numerous adaptation strategies include livestock reduction (87.3%), animal vaccination (95.2%), and Migration along with animals during the adverse climatic condition (75.4%), which the farmers employ. Lack of capital, inadequate information about climate change, and inadequate infrastructure impede adaptation. The study sug­gests that the government pursues strong policies to help livestock farmers adjust to climate unpredictability in the long run.
Relationship among the Performances of Subjective Memory, Objective Memory, and Naming in Healthy Elderly Adults
Authors: Suh Minji , Choi Hyunjoo
Published: August 2022

Purpose: The purpose of this study is to identify relationships performances among subjective memory, objective memory, and naming of healthy elderly adults. Methods: A total of 77 healthy elderly adults aged 65 or older in South Korea participated in this study. Subjective Memory Complaints Questionnaire was used to evaluate the subjective memory loss of the elderly, and the Seoul Verbal Learning Test was used to evaluate objective memory. The Korean version of Boston Naming Test was used as a confrontation naming task and Controlled Oral Word Association Test was used as a generative naming task. Results: First, the subjective memory and objective memory of the healthy elderly adults showed a significant correlation, and the naming performances of the healthy elderly adults showed a significant correlation with the recall ability of the objective memory. In addition, it could be seen that the ‘supermarket’ category of the generative naming was a task that reflected both subjective and objective memory of the general elderly. Conclusion: The results of this study are meaningful in that they have identified the usefulness of subjective memory reporting at clinical sites and in that they have presented basic data on the subjective·objective memory and the naming performances of healthy elderly adults.
Role of Diet in the Pathogenesis of Colorectal Polyps and Cancer
Authors:   Department of Internal Medicine, George Washington University, Washington DC, United States, Patel Ankit , Houston Kevin ,   Department of Internal Medicine, Virginia Commonwealth University, Richmond VA, United States, Saadeh Michael ,   Department of Internal Medicine, Eastern Virginia Medical School, Norfolk VA, United States, Vilela Ana ,   Department of Internal Medicine, Eastern Virginia Medical School, Norfolk VA, United States, Yoo Byung Soo ,   Division of Gastroenterology, Department of Internal Medicine, Carolinas Medical Center, Charlotte NC, United States, D’Souza Steve M. ,   Department of Internal Medicine, Eastern Virginia Medical School, Norfolk VA, United States, Johnson David A. ,   Division of Gastroenterology, Department of Internal Medicine, Eastern Virginia Medical School, Norfolk VA, United States
Published: August 2022

Colorectal cancer (CRC) is currently the third most common cancer within the United States among both males and females, with increasing rates occurring in younger individuals compared to the past. Multiple environmental and social aspects including diet may be contributing to this increase in CRC rates. The aim of this review is to examine foods containing high fructose corn syrup, processed meats, and red meats which have shown to be linked to an increase in incidence of CRC. These foods can be a cause for disruption in a healthy microbiome leading to dysbiosis, which can have downstream effects on CRC formation. With the current data showing evidence of potential triggers and associations leading to CRC, more studies are needed to help directly link these dietary components and their pathways to cancer formations.
Transdiagnostic Psychopathology in a Help-Seeking Population of an Early Recognition Center for Mental Disorders: Protocol for an Experience Sampling Study
Authors: Rosen Marlene , Betz Linda T , Montag Christian , Kannen Christopher , Kambeitz Joseph
Published: August 2022

Background

Prevention in psychiatry provides a promising way to address the burden of mental illness. However, established approaches focus on specific diagnoses and do not address the heterogeneity and manifold potential outcomes of help-seeking populations that present at early recognition services. Conceptualizing the psychopathology manifested in help-seeking populations from a network perspective of interacting symptoms allows transdiagnostic investigations beyond binary disease categories. Furthermore, modern technologies such as smartphones facilitate the application of the Experience Sampling Method (ESM).

Objective

This study is a combination of ESM with network analyses to provide valid insights beyond the established assessment instruments in a help-seeking population.

Methods

We will examine 75 individuals (aged 18-40 years) of the help-seeking population of the Cologne early recognition center. For a maximally naturalistic sample, only minimal exclusion criteria will be applied. We will collect data for 14 days using a mobile app to assess 10 transdiagnostic symptoms (ie, depressive, anxious, and psychotic symptoms) as well as distress level 5 times a day. With these data, we will generate average group-level symptom networks and personalized symptom networks using a 2-step multilevel vector autoregressive model. Additionally, we will explore associations between symptom networks and sociodemographic, risk, and resilience factors, as well as psychosocial functioning.

Results

The protocol was designed in February 2020 and approved by the Ethics Committee of the University Hospital Cologne in October 2020. The protocol was reviewed and funded by the Köln Fortune program in September 2020. Data collection began in November 2020 and was completed in November 2021. Of the 258 participants who were screened, 93 (36%) fulfilled the inclusion criteria and were willing to participate in the study. Of these 93 participants, 86 (92%) completed the study. The first results are expected to be published in 2022.

Conclusions

This study will provide insights about the feasibility and utility of the ESM in a help-seeking population of an early recognition center. Providing the first explorative phenotyping of transdiagnostic psychopathology in this population, our study will contribute to the innovation of early recognition in psychiatry. The results will help pave the way for prevention and targeted early intervention in a broader patient group, and thus, enable greater intended effects in alleviating the burden of psychiatric disorders.

International Registered Report Identifier (IRRID)

DERR1-10.2196/35206
Pengaruh Pendidikan Teman Sebaya Terhadap Kemampuan Perilaku Cuci Tangan pada Anak Usia Pra Remaja
Authors: Ristanti Herlina , Mulyani Sri , Madyaningrum Ema
Published: August 2022

Background: Closeness of students during preteen years can be a facility to improve hygienic and healthy life behaviors. Peer education is an effort for improving experience leading to a change of behavior on the group member. One of behaviors mastered by junior high school students related to a clean and healthy life behaviors is hand-washing.Objective: To investigate the influence of peer education toward the ability of hand-washing on preteens in SMPN 5 Depok, Sleman.Methods: This was a quasi-experimental research with one group pretest and posttest design. The respondents in this research were 128 students of the seventh grade of SMPN 5 Depok. This research used the hand-washing behavior questionnaire and observation sheet. The paired sample t-test was used in order to know the hand-washing behavior before and after peer education. Observation sheet was analyzed using one-way anova test to compare the average observation for three weeks.Results: The average score of hand-washing before and after peer education were 48,177 ± SD = 7,099 and 82,838 ± SD = 6,536 respectively. There was a significant difference before and after peer education toward the hand-washing behavior with p-value = 0,0001.Conclusion: Peer education influenced the progress of hand-washing on preteen children.ABSTRAKLatar Belakang: Kedekatan siswa pada usia pra remaja dapat menjadi fasilitas untuk meningkatkan perilaku hidup bersih dan sehat. Pendidikan teman sebaya merupakan suatu wadah untuk meningkatkan pengalaman yang menyebabkan perubahan perilaku pada anggota kelompok. Salah satu perilaku yang dikuasai oleh siswa SMP terkait perilaku hidup bersih dan sehat yaitu cuci tangan.Tujuan: Mengetahui pengaruh pendidikan teman sebaya terhadap kemampuan perilaku cuci tangan pada anak usia remaja di SMPN 5 Depok, Sleman.  Metode: Penelitian ini merupakan penelitian quasi experimental dengan rancangan one group pretest and posttest design. Responden pada penelitian ini adalah siswa kelas VII SMPN 5 Depok yang berjumlah 128 orang. Penelitian ini menggunakan kuesioner perilaku mencuci tangan dan lembar observasi. Analisis penelitian menggunakan paired sample t-test untuk mengetahui pengaruh perilaku cuci tangan sebelum dan sesudah pendidikan teman sebaya. Lembar observasi dianalisis menggunakan uji anova satu arah untuk membandingkan rata-rata observasi selama tiga minggu.Hasil : Hasil rata-rata skor perilaku cuci tangan sebelum pendidikan teman sebaya sebesar 48,177 ± SD= 7,099 dan sesudah sebesar 82,838 ± SD= 6,536. Terdapat perbedaan yang signifikan sebelum dan sesudah pendidikan teman sebaya terhadap perilaku cuci tangan dengan p-value = 0,0001.Kesimpulan: Pendidikan teman sebaya berpengaruh terhadap peningkatan perilaku cuci tangan pada anak usia pra-remaja.
Efektivitas Biaya Program Berbasis Keperawatan: Pemasangan Akses Vena Sentral Melalui Perifer Yang Dipandu Ultrasonografi
Authors: Kusumawati Anastasia Sari , Sukihananto Sukihananto
Published: August 2022

Background: Vein access is frequent procedures in hospital care. Repeated attempts to cannulate small veins can cause considerable distress for patients and took considerable amount of staff's time. Peripherally inserted central catheter recommended replacing a long term peripheral catheter. The responsibility for the insertion of peripheral catheters has shifted from specially trained nurses to skilled nurses. A peripherally inserted central venous catheter is recommended to replace long term peripherally catheters.Objective: This literature study goals to give a view in cost effectiveness venous access practices using ultrasound-guided practices and traditional cannulate practices nurse-based.Method: The method used in this paper is literature review related topics.Result: There are several studies that demonstrate the cost effectiveness of venous access practices using nursing-based ultrasound guidelines.Conclusion: Result shows by implementation of nursing based on ultrasound-guided peripheral venous access gave more effective approach than traditional practices. ABSTRAKLatar belakang: Akses vena sering dilakukan dalam perawatan di rumah sakit. Upaya berulang untuk memasang kateter vena kecil dapat menyebabkan tekanan mental yang besar bagi pasien dan membutuhkan waktu staf cukup banyak. Tanggung jawab pemasangan kateter perifer telah bergeser dari perawat sangat terampil ke perawat terampil. Pemasangan akses vena sentral melalui perifer direkomendasikan untuk mengganti kateter perifer jangka panjang. Tujuan: Tinjauan studi literatur ini diharapkan dapat memberi gambaran tentang efektivitas biaya praktik akses vena dengan menggunakan panduan ultrasonografi berbasis keperawatan. Metode: Metode yang digunakan dalam penulisan ini adalah literature review terkait topik.Hasil: Terdapat beberapa penelitian yang membuktikan efektivitas biaya praktik akses vena dengan menggunakan panduan ultrasonografi berbasis keperawatan.Kesimpulan: Hasil menunjukkan bahwa penerapan akses vena perifer dengan panduan ultrasonografi berbasis keperawatan, mampu menjadi pendekatan yang lebih efektif dibandingkan praktik tradisional.
Faktor-Faktor Yang Beresiko Terjadinya Pasien Stagnan Di Instalasi Gawat Darurat Rumah Sakit
Authors: Damayanti Puti , Sutono Sutono
Published: August 2022

Background: Fast response is needed in the Emergency Room (ER). However, the high number of patient visit leads stagnant patient in ER. The impact of patient stagnant  can affect other patient safety and quality of services in the hospital.Objective: Identify factors related to the presence of stagnant patients in the ER of  Dr. Sardjito General Hospital.Methods: This study used a retrospective case control method. The total samples in this study is 87 samples and divided into two groups, 29 samples for non-stagnant group and 58 samples for stagnant group. Data collection used non-probability sample with purposive sampling method for the sample selection. Sampling was carried out at 16 to 28 January 2015 in ER of Dr. Sardjito General Hospital. This research uses Chi Square and logistic regression to data analyze.Results: Risk factors to stagnant patients are the availability of beds (p: <0.001), corresponding ward grouping disease patients (p: 0.021), fogging (p: 0.008), age of the patient (p: <0.001), patients came hours ( p: 0.019) and the number of actions in Emergency Room (p: 0.002). From the results of logistic regression the most influential factor for the patients is the availability of beds (15 times more risky).Conclusions: Factors related to patients are the availability of a stagnant, according grouping wards patient's disease, fogging, patient age, patient coming hours and the number of actions.ABSTRAKLatar belakang: Pelayanan yang cepat sangat dibutuhkan di IGD. Akan tetapi kondisi kunjungan pasien di IGD yang cukup banyak dapat menimbulkan pasien stagnan di IGD . Dampak pasien yang stagnan mempengaruhi keselamatan pasien yang lain dan mutu pelayanan rumah sakit tersebut.Tujuan: Mengetahui faktor-faktor yang berisiko terjadinya pasien yang stagnan di Instalasi Gawat Darurat Rumah Sakit Umum Pusat Dr. Sardjito Yogyakarta.Metode: penelitian ini menggunakan metode case control retrospektif dengan jumlah sampel masing-masing 29 sampel untuk kelompok non-stagnan dan 58 sampel untuk kelompok stagnan. Pengambilan data dilakukan dengan cara non-probability sample dengan metode purposive sampling. Pengambilan sampel dilakukan pada 16-28 Januari 2015 di IGD RSUP Dr. Sardjito Yogyakarta. Analisa yang digunakan di dalam penelitian ini antara lain Chi Square dan regresi logistik.Hasil:  Faktor-faktor yang berisiko pasien stagnan antara lain ketersediaan tempat (p:<0,001), bangsal sesuai pengelompokkan penyakit pasien (p:0,021), fogging(p:0,008), usia pasien(p:<0,001), jam datang pasien(p:0,019) dan jumlah tindakan(p:0,002). Dari hasil regresi logistik faktor yang paling berisiko dengan terjadinya pasien stagnan adalah ketersediaan tempat (15 kali lebih berisiko)Kesimpulan : Faktor-faktor yang berhubungan dengan pasien stagnan antara lain ketersediaan tempat, bangsal sesuai pengelompokkan penyakit pasien, fogging, usia pasien, jam datang pasien dan jumlah tindakan.