bluuebunny/crossref_metadata_2025 huggingface dataset:
Simulation and experiment researches on rubber pad forming of two-step channels by different dies
Authors: Fei Teng , Hongyu Wang , Guodong Wang , Lei Jiang , Juncai Sun , Jie Sun , Shunhu Zhang
Published: September 2021

Rubber pad forming is one of advance processing technologies. With both rubber pad and die, the sheet metal is stamped into the required shapes. The shapes of the die directly affect the final shapes of the channels on the sheet. With the developments of micro-channels, a new kind of two-step channels is concerned gradually in many fields. Since there are waved structures in these channels, many beneficial functions are caused. However, the manufacturing of this new kind channels by rubber pad forming are still not meticulously researched. This article is focused on the rubber pad forming process of different two-step channels. Different two-step channels are designed and made. Based on both FEM and physical experiments, the forming processes of these new channels are researched. The forming results are discussed and compared with each other, the best design strategy is also proposed through results.
Experimental investigation of scavenging in two-stroke engines using continuous CO2 sampling
Authors: Bajwa Abdullah U , Patterson Mark , Jacobs Timothy J
Published: September 2021

In internal combustion engines, the chemical composition of the trapped fuel-air-residual gas mixture controls the nature of combustion, which, in turn, determines the characteristics of the ensuing emissions and work production processes. Therefore, knowledge of the trapped mixture’s composition is critical for reliably predicting and controlling engine performance, emissions, and efficiency. A good index of the overall trapped mixture composition is the trapped equivalence ratio. Unfortunately, in two-stroke engines, it is unfeasible to accurately determine the trapped equivalence ratio using traditional intake flow measurements and exhaust emissions data. This limitation arises from the simultaneous occurrence of intake and exhaust processes in two-stroke engines, which causes: (1) exhaust emissions to be diluted by excess fresh air that was supplied for achieving effective gas exchange, that is trapping inefficiencies and (2) a significant fraction of combustion products to stay back in the cylinder as residual gas, that is scavenging inefficiencies. The current paper presents an experimental study carried out on a cross-scavenged, lean-burn, natural-gas, two-stroke engine to characterize its scavenging performance, thus paving the way for trapped equivalence ratio computation. CO2 is used as a tracer for combustion products, and its concentration is tracked in the combustion chamber and exhaust manifold on a crank-angle-resolved basis using high-speed nondispersive infrared sensors. The changes in cylinder CO2 concentration before and after gas exchange are used to determine the trapped residual fraction and various features of the exhaust CO2“wave” are used to explain the temporal progression of the gas exchange process. The presented results show the effects of changes in engine operation (speed, load, and spark-timing) on the engine’s scavenging efficiency. Speed and load changes are found to have the most pronounced effects, which result from changes in port open duration and phasing of reflected waves in the exhaust.
Decent Work Among People of Color: The Moderating Role of Critical Consciousness
Authors: Autin Kelsey L. , Williams Tiffany R. , Allan Blake A. , Herdt Megan E.
Published: September 2021

The present study examined critical consciousness in a sample of 476 adults of color from a Psychology of Working perspective. Using structural equation modeling, we tested three components of critical consciousness—perceived inequality, egalitarian beliefs, and sociopolitical participation—as moderators of relations between marginalization, economic constraints, work volition, career adaptability, and decent work. As hypothesized, perceived inequality and sociopolitical participation moderated paths from marginalization to career adaptability, work volition, and decent work. Perceived inequality moderated paths from economic constraints to career adaptability and decent work, but in inconsistent directions. We discuss practical implications and future research directions. Our results contribute to the growing support for the Psychology of Working Theory.
Exploring Barriers to the Development of Home Health Care in Iran: A Qualitative Study
Authors: Kianian Toktam , Lotfi Mojgan , Zamanzadeh Vahid , Rezayan Ahad , Hazrati Maryam , Pakpour Vahid
Published: September 2021

Changes in society’s needs have increased individuals’ desire to be independent and receive health care at home. Home health care (HHC) is one of the main components of realizing these ambitions. However, there is a serious concern about the state of development of the HHC industry in Iran and the quality of its services. This study aims to explore the barriers to the development of HHC in Iran. This qualitative study with a conventional content analysis approach was conducted in Tabriz, Iran, from October 2019 to March 2020. Twenty-one key stakeholders including nurses, home health directors, physicians, policy-makers, patients, and their families participated in the study. Participants were selected using purposive sampling. Data were collected using a focus group discussion (FGD) and 18 semi-structured in-depth interviews and analyzed using Graneheim and Lundman’s techniques. Data collection continued until saturation was reached. Three main themes emerged from the analysis: quality management challenges, insufficient infrastructure and policy, and political issues. The findings show that applying standard strategies to monitor the quality of services, paying attention to infrastructure, and having a clear framework for policy-making are beneficial for the development of the HHC industry. Health managers can monitor and upgrade the quality of HHC services by defining standards and assessment indicators, as well as providing comprehensive data. It is suggested that legislators consider the principles of community-based accountability, justice, and accessibility their top priority. Moreover, policymakers need to develop a roadmap to reform policies to create the conditions for the development of this industry.
Determinants of a political party’s social media strategy: A comparative analysis of Tanzania’s opposition political parties’ Twitter practices
Authors: Kwayu Aikande Clement
Published: September 2021

The impact of political context on a political party’s organizing and mobilizing strategies has been noted in the literature. Opposition political parties operating in autocratic contexts look for different ways in which they can communicate, organize, and mobilize their members. Advances in digital technology and, in particular social media, have created such spaces in which political parties can communicate and mobilize their members. In Tanzania, the state has imposed repressive political restrictions against opposition parties. In this context, it would be assumed that opposition political parties would turn to social media as an alternative means for mobilization and communication due to restrictions in traditional spaces such as mainstream media and rallies. This article argues that, in Tanzania, the opposition political parties’ levels of institutionalization and resources determine their use of social media more than the repressive political context. The study compares the Twitter use of two opposition parties—Chadema and ACT-Wazalendo—in two periods between 2016 and 2020, which is the period of increasing authoritarian tendencies in the country. The study deploys a qualitative research methodology using a case study approach and collection of data from the parties’ practices in their official Twitter accounts as well as key informant interviews.
Democracy within parties: Electoral consequences of candidate selection methods in Tanzania
Authors: Sulley Consolata R
Published: September 2021

A large body of literature exists around the study of elections. What often goes unnoticed, however, is that elections take place between and within parties. While the former has received significant attention, the latter has not. This is true of African elections generally, and Tanzanian elections specifically. This study examines one important aspect of intra-party democracy – the process of candidate selection. Particular attention is paid to the electoral outcomes of selection methods in two purposively selected political parties in Tanzania, the ruling Chama cha Mapinduzi (CCM) and the opposition Chama cha Demokrasia na Maendeleo (CHADEMA). Based on an examination of party nomination rules, electoral observation reports, the media, and stakeholder interviews, I argue that candidate selection methods in both parties are, by design, undemocratic. Non-restrictive candidacy requirements, exclusive selectorates, centralised selection and appointment, rather than democratic voting procedures, dominate selection processes. Undemocratic candidate selection has affected the share of votes and seats received by both parties in elections.
Documentary imaginary: Production and audience research of The Act of Killing and The Look of Silence
Authors: Hill Annette
Published: September 2021

Oppenheimer describes The Act of Killing as a ‘documentary about the imagination. We are documenting the ways we imagine ourselves, the ways we know ourselves’. This research analyses the documentary films The Act of Killing (Director Oppenheimer, co-directors Christine Cynn and anonymous 2012) and The Look of Silence (director Oppenheimer 2014), and the documentary imaginary. The research combines normally separate sites of analysis in production and audience studies in order to understand the power of documentary and the spectrum of social stories we inhabit. The article asks: how do the films document and imagine fear and impunity in memories of the genocide, and how do audiences engage with this documentary imaginary? Particular focus is paid towards the endings of the two documentary films and how audiences in this study reflect on the absence of justice for the victims of the genocide. Through the empirical research, we take a journey with the director and his film making process, understanding the lengthy and complex filming for the two documentaries in Indonesia. The films signal Oppenheimer’s political and ethical commitment towards victim recognition, the possibility and impossibility of forgiveness, and the challenge of reconciliation between victims and perpetrators. The filmmaker’s journey is intertwined with the enactments of the genocide by the perpetrators in their own surreal ways of imagining themselves, and the experience of victims seeking recognition. Audiences become intertwined in these journeys, finding along the way a critically productive space for documentary and the imaginary.
Self-segregation strategies through school choice in Chile: A middle-class domain?
Authors: Carrasco Alejandro , Mendoza Manuela , Flores Carolina
Published: September 2021

Sociological research has shown that marketized educational systems favour middle-class families’ self-segregation strategies through school choice and, consequently, the reproduction of their social advantage over poorer families. Drawing on Pierre Bourdieu’s concepts of capitals, habitus and strategy, we analyse quantitative and ethnographic data on parents’ school choice from Chile to introduce nuances to this argument, evincing more extended and complex mechanisms of self-segregation in the Chilean marketized educational system. We found that not only middle-class parents but also parents from different socioeconomic groups displayed self-segregating school choice strategies. We also found that these strategies were performed both vertically (in relation to other social classes) and horizontally (in relation to other groups within the same social class). These findings unwrap a possible stronger effect of the Chilean school choice system over segregation.
COVID-19 in India: A Comparative Analysis of the Kerala and Gujarat Development Models’ Initial Responses
Authors: Israelsen Shelli , Malji Andrea
Published: September 2021

Significant variations in infection, testing, and mortality rates have exposed key differences in the initial COVID-19 response by Indian states. At the onset of the pandemic, states like Gujarat, known for its large economic output, suffered high COVID-19 case fatality rates, a disorganized response, and poor access to healthcare. In contrast, Kerala, a less industrialized state on India’s southwestern coast, experienced low infection rates and fatalities. The low case fatality rate was accompanied by widespread access to care, extensive testing, and an organized response by the state. The emergence of the COVID-19 pandemic provides an opportunity to compare how the Gujarat and Kerala models performed. Since 2000, the Gujarat model has emphasized industrialization and economic development, often at the expense of social development. In contrast, the Kerala model emphasizes social development, often at the expense of economic development. This article analyses the initial response to COVID-19 by Kerala and Gujarat and finds that the Kerala model and its emphasis on social development helped the state respond more effectively to the first wave of the pandemic compared to Gujarat.
A contextual needs assessment of families with home invasive mechanical ventilation patients: A qualitative study
Authors: Esmaeili Maryam , Asgari Parvaneh , Dehghan Nayeri Nahid , Bahramnezhad Fatemeh , Fattah Ghazi Samrand
Published: September 2021

Objectives

With the advancement of technology the number of patients surviving critical illness has increased. Home mechanical ventilation (HMV) is a growing option for patients requiring long-term mechanical ventilation. Caring for these patients is demanding and challenging. The aim of this study was to explore family caregivers’(carers) needs when providing care to adult patients under HMV from the perspective of nurses, home care attendants, and the caregivers themselves.

Methods

Overall, 15 participants (nine carers, three home nurses, and three home care attendants) were selected by purposive sampling. Data were collected by in-depth semi-structured interviews and structured observation. Finally, data were analyzed through conventional content analysis with MAXQDA software.

Results

Three categories of carers’needs were identified, including educational needs (basic and emergencies), psychological needs, and economic needs. In addition, since the needs, feelings, and views of caregivers change over time, the noted needs were divided into three periods: Pre-discharge preparation, initial transition from hospital to home, and appropriate long-term follow-up.

Conclusion

The study results showed that the families of patients under invasive HMV require a standard discharge plan based on their care needs, financial concerns, and psychological screening before discharge as well as a suitable long-term follow-up plan in collaboration with a multidisciplinary treatment team, insurance providers, and home care services.
Protesting Populist Knowledge Practices: Collective Effervescence at the March for Science London
Authors: Stephens Neil , Vrikki Photini , Riesch Hauke , Martin Olwenn
Published: September 2021

On 22 April 2017, 10,000 people joined the March for Science London, one of 600 events globally asserting the importance of science against post-truth. Here we report an online and on-the-ground observational study of the London event in its distinct, post-Brexit referendum context. We analyse the motives for marchers’ attendance, and their collective enactment of what science is and why and by what it is threatened. Drawing upon Interaction Ritual Theory and the concept of civic epistemology, we develop the notion of populist knowledge practices to capture the ‘other’ that marchers defined themselves against. We detail how this was performed, and how it articulated a particular vision for science–society relations in Britain. In closing, we argue that the March for Science is one in a chain of anti-populist activist events that retains collective effervescence while transcending specific framings.
Essential Oils of the Leaf and Stem of Polyalthia viridis Craib and Their Biological Activities
Authors: Son Ninh T. , Le Tuan A. , Thuy Dinh T. T. , Nguyen Dinh L. , Tuyen Tran T. , Hoang Thi Minh D. , Nguyen Manh H.
Published: September 2021

Plants of the genus Polyalthia can be seen as a rich resource of essential oils type terpenoids. In this study, the essential oils obtained by hydro-distillation from the leaf and stem of Polyalthia viridis were analysed by gas chromatography/mass spectrometry–flame ionization detection. Thirty-nine constituents (95.3%) were identified in the leaf oils and 42 constituents (90.9%) in the stem oils. Sesquiterpene hydrocarbons and oxygenated sesquiterpenes were the main constituents of both oils, in which 2 sesquiterpene hydrocarbons, germacrene D (45.1%-47.4%) and bicyclogermacrene (8.2%-17.1%), were the 2 major compounds. The stem oils inhibited the growth of 3 cancer cell lines, HepG2, MCF7, and A549, with half inhibitory concentration (IC50) values of 56.7 to 68.4 μg/mL. The stem oils also successfully suppressed the growth of the filamentous fungus Aspergillus niger and the yeast Candida albicans with minimum inhibitory concentration values of 50 μg/mL. P viridis oils suppressed NO production in lipopolysaccharide-stimulated BV2 microglial cells, with IC50 values of 57.6 to 76.7 μg/mL.
Differentiation of Benign From Malignant Sinonasal Masses Using Diffusion Weighted Imaging and Dynamic Contrast Enhanced Magnetic Resonance Imaging
Authors: Daga Radhika , Kumar Jyoti , Pradhan Gaurav , Meher Ravi , Malhotra Vikas , Khurana Nita
Published: September 2021

Background

The sinonasal region is affected by a variety of neoplasms. A differentiation between benign and malignant masses is essential both for management and prognostication. Morphological analysis often does not allow this differentiation.

Objectives

This article aims to assess the value of advanced MRI (diffusion [DWI] and dynamic contrast enhanced MRI [DCE-MRI]) in differentiation of benign and malignant sinonasal masses.

Methods

This prospective study included 40 patients with sinonasal masses who underwent advanced MR on 3T MR scanner. The lesions were analyzed based on morphological characteristics, qualitative, quantitative diffusion parameters, and time signal intensity curves. Apparent diffusion coefficient (ADC) values were acquired using b values of 50 and 1000 s/mm2. The accuracy of DWI, DCE-MRI, and combined DWI/DCE-MRI in differentiating benign from malignant sinonasal masses were analyzed.

Results

Perineural extension and growth pattern of the tumor were the best morphological discriminators. Mean ADC values for benign and malignant lesions were 1.675  ±  0.561 and 0.903  ±  0.405  × 10−3 mm2/sec, ,respectively. ROC revealed that ADC cutoff value of 1.005  × 10−3 mm2/sec provided an accuracy of 92.5% in differentiating benign from malignant masses ( P value <.01). On excluding the benign vascular masses (Juvenile Nasopharyngeal Angiofibroma and hemangioma), the time signal intensity curve showed 78% accuracy ( P value <.001). The highest diagnostic performance was achieved by combining DWI and DCE-MRI (95% accuracy).

Conclusion

DWI has higher accuracy than DCE-MRI. Quantitative DWI is preferable over qualitative DWI. Accuracy of DCE-MRI can be increased by excluding vascular masses with characteristic imaging features. DWI and DCE-MRI have the highest accuracy when used in combination than either of them alone in differentiating benign from malignant sinonasal masses.
Dissection of the Genetic Basis of Rice Panicle Architecture Using a Genome-wide Association Study
Authors: Bai Shaoxing , Hong Jun , Li Ling , Su Su , Li Zhikang , Wang Wensheng , Zhang Fengli , Liang Wanqi , Zhang Dabing
Published: September 2021

Panicle architecture is one of the major factors influencing productivity of rice crops. The regulatory mechanisms underlying this complex trait are still unclear and genetic resources for rice breeders to improve panicle architecture are limited. Here, we have performed a genome-wide association study (GWAS) to analyze and identify genetic determinants underlying three panicle architecture traits. A population of 340 rice accessions from the 3000 Rice Genomes Project was phenotyped for panicle length, primary panicle number and secondary branch number over two years; GWAS was performed across the whole panel, and also across the japonica and indica sub-panels. A total of 153 quantitative trait loci (QTLs) were detected, of which 5 were associated with multiple traits, 8 were unique to either indica or japonica sub-panels, while 37 QTLs were stable across both years. Using haplotype and expression analysis, we reveal that genetic variations in the OsSPL18 promoter significantly affect gene expression and correlate with panicle length phenotypes. Three new candidate genes with putative roles in determining panicle length were also identified. Haplotype analysis of OsGRRP and LOC_Os03g03480 revealed high association with panicle length variation. Gene expression of DSM2, involved in abscisic acid biosynthesis, was up-regulated in long panicle accessions. Our results provide valuable information and resources for further unravelling the genetic basis determining rice panicle architecture. Identified candidate genes and molecular markers can be used in marker-assisted selection to improve rice panicle architecture through molecular breeding.
Analysis of microbial populations in plastic–soil systems after exposure to high poly(butylene succinate-co-adipate) load using high-resolution molecular technique
Authors: Tanunchai Benjawan , Juncheed Kantida , Wahdan Sara Fareed Mohamed , Guliyev Vusal , Udovenko Maria , Lehnert Ann-Sophie , Alves Eliane Gomes , Glaser Bruno , Noll Matthias , Buscot François , Blagodatskaya Evgenia , Purahong Witoon
Published: September 2021

Background

Bio-based and biodegradable plastics are considered as plastics of the future owing to their ability to decompose under various environmental conditions. However, their effects on the soil microbiome are poorly characterised. In this study, we aimed to investigate the effects of an important bio-based and biodegradable plastic, polybutylene succinate-co-adipate (PBSA), on soil microbial diversity and community composition using high-resolution molecular technique (Illumina sequencing) targeting all three microbial domains: archaea, bacteria, and fungi.

Results

Adding high load of PBSA to soil (6% (w/w)) caused a significant decline in archaeal (13%) and fungal (45%) richness and substantial changes in both bacterial (Proteobacteria, Actinobacteria, and Acidobacteria) and fungal (Eurotiomycetes, Sordariomycetes, Leotiomycetes, and Dothideomycetes) community composition compared with no PBSA addition to soil. The combined effects of PBSA and (NH4)2SO4fertilisation on the soil microbiome were much greater than the effects of PBSA alone. We only detected opportunistic human pathogens in low abundance on PBSA and in the surrounding soil. However, some plant pathogenic fungi were detected and/or enriched on the PBSA films and in surrounding soil. Apart from plant pathogens, many potential microbial control agents and plant growth-promoting microorganisms were also detected/enriched owing to PBSA addition. Adding high load of PBSA together with (NH4)2SO4fertilisation can either eliminate some plant pathogens or enrich specific pathogens, especiallyFusarium solani, which is economically important.

Conclusions

We conclude that high load of bio-based and biodegradable PBSA plastic may negatively affect soil microbiome.
Pain expectation and avoidance in the social context: an electrophysiological study
Authors: Piedimonte Alessandro , Zamfira Denisa Adina , Guerra Giulia , Vighetti Sergio , Carlino Elisa
Published: September 2021

Contingent negative variation (CNV) is an informative electrophysiological measure of pain anticipation showing higher amplitudes when highly painful stimulation is expected while presenting lower amplitudes when low painful stimulation is expected. Two groups of participants were recruited: one group expected and received an electrical stimulation of different intensities while being alone in the room (i.e. without social context), while a second group performed the same experiment with an observer in the room (i.e. with social context). Lower pain ratings and slower reaction times were observed in the group with social context and these results were accompanied in this group by a lower amplitude in the early component of the CNV as well as a lower amplitude of the later component of the wave. These results show that CNV can be considered a precise measure of central elaboration of pain anticipation explaining both its perceptual and motor components.
Elucidating bacterial adhesion to mucosal surface by an original AFM approach
Authors: Dunker Karen , de la Torre Canny Sol Gomez , Nordgård Catherine Taylor , Dague Etienne , Formosa-Dague Cécile , Bakke Ingrid , Sletmoen Marit
Published: September 2021

Background

Fish skin represents an ancient vertebrate mucosal surface, sharing characteristics with other mucosal surfaces including those of the intestine. The skin mucosa is continuously exposed to microbes in the surrounding water and is therefore important in the first line defense against environmental pathogens by preventing bacteria from accessing the underlying surfaces. Understanding the microbe-host interactions at the fish skin mucosa is highly relevant in order to understand and control infection, commensalism, colonization, persistence, infection, and disease. Here we investigate the interactions between the pathogenic bacteria Aeromonas salmonicida (A. salmonicida) and Yersinia ruckeri (Y. ruckeri), respectively, and the skin mucosal surface of Atlantic salmon fry using AFM force spectroscopy.

Results

The results obtained revealed that when retracting probes functionalized with bacteria from surfaces coated with immobilized mucins, isolated from salmon mucosal surfaces, rupture events reflecting the disruption of adhesive interactions were observed, with rupture strengths centered around 200 pN. However, when retracting probes functionalized with bacteria from the intact mucosal surface of salmon fish fry no adhesive interactions could be detected. Furthermore, rheological measurements revealed a near fluid-like behavior for the fish fry skin mucus. Taken together, the experimental data indicate that the adhesion between the mucin molecules within the mucous layer may be significantly weaker than the interaction between the bacteria and the mucin molecules. The bacteria, immobilized on the AFM probe, do bind to individual mucins in the mucosal layer, but are released from the near fluid mucus with little resistance upon retraction of the AFM probe, to which they are immobilized.

Conclusion

The data provided in the current paper reveal that A. salmonicida and Y. ruckeri do bind to the immobilized mucins. However, when retracting the bacteria from intact mucosal surfaces, no adhesive interactions are detected. These observations suggest a mechanism underlying the protective function of the mucosal surface based on the clearing of potential threats by adhering them to loosely attached mucus that is subsequently released from the fish skin.
Effect of extremely low frequency magnetic fields on oxidative balance in rat brains subjected to an experimental model of chronic unpredictable mild stress
Authors: Quesnel-Galván Leticia R. , Torres-Durán Patricia V. , Elías-Viñas David , Verdugo-Díaz Leticia
Published: September 2021

Background

There has been an increasing interest in researching on the effects of extremely low-frequency magnetic fields on living systems. The mechanism of action of extremely low-frequency magnetic fields on organisms has not been established. One of the hypotheses is related to induce changes in oxidative balance. In this study, we measured the effects of chronic unpredictable mild stress induced-oxidative balance of rat’s brain exposed to extremely low-frequency magnetic fields.

Methods

A first experiment was conducted to find out if 14 days of chronic unpredictable mild stress caused oxidative unbalance in male Wistar rat’s brain. Catalase activity, reduced glutathione concentration, and lipoperoxidation were measured in cerebrum and cerebellum. In the second experiment, we investigate the effects of 7 days extremely low-frequency magnetic fields exposure on animals stressed and unstressed.

Results

The main results obtained were a significant increase in the catalase activity and reduced glutathione concentration on the cerebrum of animals where the chronic unpredictable mild stress were suspended at day 14 and then exposed 7 days to extremely low-frequency magnetic fields. Interestingly, the same treatment decreases the lipoperoxidation in the cerebrum. The stressed animals that received concomitant extremely low frequency magnetic fields exposure showed an oxidative status like stressed animals by 21 days. Thus, no changes were observed on the chronic unpredictable mild stress induced-oxidative damage in the rat’s cerebrum by the extremely low-frequency magnetic field exposure together with chronic unpredictable mild stress.

Conclusions

The extremely low-frequency electromagnetic field exposure can partially restore the cerebrum antioxidant system of previously stressed animals.
Mapping freezing tolerance QTL in alfalfa: based on indoor phenotyping
Authors: Adhikari Laxman , Makaju Shiva O. , Lindstrom Orville M. , Missaoui Ali M.
Published: September 2021

Background

Winter freezing temperature impacts alfalfa (Medicago sativa L.) persistence and seasonal yield and can lead to the death of the plant. Understanding the genetic mechanisms of alfalfa freezing tolerance (FT) using high-throughput phenotyping and genotyping is crucial to select suitable germplasm and develop winter-hardy cultivars. Several clones of an alfalfa F1 mapping population (3010 x CW 1010) were tested for FT using a cold chamber. The population was genotyped with SNP markers identified using genotyping-by-sequencing (GBS) and the quantitative trait loci (QTL) associated with FT were mapped on the parent-specific linkage maps. The ultimate goal is to develop non-dormant and winter-hardy alfalfa cultivars that can produce extended growth in the areas where winters are often mild.

Results

Alfalfa FT screening method optimized in this experiment comprises three major steps: clone preparation, acclimation, and freezing test. Twenty clones of each genotype were tested, where 10 samples were treated with freezing temperature, and 10 were used as controls. A moderate positive correlation (r ~ 0.36, P < 0.01) was observed between indoor FT and field-based winter hardiness (WH), suggesting that the indoor FT test is a useful indirect selection method for winter hardiness of alfalfa germplasm. We detected a total of 20 QTL associated with four traits; nine for visual rating-based FT, five for percentage survival (PS), four for treated to control regrowth ratio (RR), and two for treated to control biomass ratio (BR). Some QTL positions overlapped with WH QTL reported previously, suggesting a genetic relationship between FT and WH. Some favorable QTL from the winter-hardy parent (3010) were from the potential genic region for a cold tolerance gene CBF. The BLAST alignment of a CBF sequence of M. truncatula, a close relative of alfalfa, against the alfalfa reference showed that the gene’s ortholog resides around 75 Mb on chromosome 6.

Conclusions

The indoor freezing tolerance selection method reported is useful for alfalfa breeders to accelerate breeding cycles through indirect selection. The QTL and associated markers add to the genomic resources for the research community and can be used in marker-assisted selection (MAS) for alfalfa cold tolerance improvement.
Influencing factors of Barthel index scores among the community-dwelling elderly in Hong Kong: a random intercept model
Authors: Pan Hao , Zhao Yang , Wang Hailiang , Li Xinyue , Leung Eman , Chen Frank , Cabrera Javier , Tsui Kwok Leung
Published: September 2021

Background

Barthel Index (BI) is one of the most widely utilized tools for assessing functional independence in activities of daily living. Most existing BI studies used populations with specific diseases (e.g., Alzheimer’s and stroke) to test prognostic factors of BI scores; however, the generalization of these findings was limited when the target populations varied.

Objectives

The aim of the present study was to utilize electronic health records (EHRs) and data mining techniques to develop a generic procedure for identifying prognostic factors that influence BI score changes among community-dwelling elderly.

Methods

Longitudinal data were collected from 113 older adults (81 females; mean age = 84 years, SD = 6.9 years) in Hong Kong elderly care centers. Visualization technologies were used to align annual BI scores with individual EHRs chronologically. Linear mixed-effects (LME) regression was conducted to model longitudinal BI scores based on socio-demographics, disease conditions, and features extracted from EHRs.

Results

The visualization presented a decline in BI scores changed by time and health history events. The LME model yielded a conditional R2 of 84%, a marginal R2 of 75%, and a Cohen’s f2 of 0.68 in the design of random intercepts for individual heterogeneity. Changes in BI scores were significantly influenced by a set of socio-demographics (i.e., sex, education, living arrangement, and hobbies), disease conditions (i.e., dementia and diabetes mellitus), and EHRs features (i.e., event counts in allergies, diagnoses, accidents, wounds, hospital admissions, injections, etc.).

Conclusions

The proposed visualization approach and the LME model estimation can help to trace older adults’ BI score changes and identify the influencing factors. The constructed long-term surveillance system provides reference data in clinical practice and help healthcare providers manage the time, cost, data and human resources in community-dwelling settings.
Impact of switch from tenofovir disoproxil fumarate-based regimens to tenofovir alafenamide-based regimens on lipid profile, weight gain and cardiovascular risk score in people living with HIV
Authors: Plum Pierre-Emmanuel , Maes Nathalie , Sauvage Anne-Sophie , Frippiat Frédéric , Meuris Christelle , Uurlings Françoise , Lecomte Marianne , Léonard Philippe , Paquot Nicolas , Fombellida Karine , Vaira Dolores , Moutschen Michel , Darcis Gilles
Published: September 2021

Background

As cardiovascular diseases represent the main cause of non-AIDS related death in people living with HIV (PLWH) with undetectable viral load, we evaluated lipid profile, weight gain and calculated cardiovascular risk change after switching from tenofovir disoproxil fumarate (TDF)-based regimens to tenofovir alafenamide (TAF)-based regimens.

Methods

For this retrospective study, we selected HIV-infected patients with suppressed viral load who fitted in one of the two groups below: First group (TDF/TDF): Patients treated continuously with TDF-based regimens. Second group (TDF/TAF): Patients treated with TDF-regimens during at least 6 months then switched to TAF-regimens while maintaining other drugs unchanged. Available data included date of birth, gender, ethnicity, lymphocyte T CD4+ count, weight, height, blood pressure, current/ex/non-smoker, diabetes mellitus, familial cardiovascular event, lipid profile, duration and nature of antiretroviral therapy. Lipid parameters, weight and calculated cardiovascular risk using 5-year reduced DAD score algorithm [Friis-Møller et al. in Eur J Cardiovasc Prev Rehabil 17:491–501, 2010] were analyzed in each groups.

Results

Switching from TDF to TAF resulted in a significant increase in triglycerides levels, total cholesterol and HDL cholesterol. LDL cholesterol and total cholesterol/HDL ratio did not show significant changes. Calculated cardiovascular risk increased after switch from TDF- to TAF-based therapy.

Conclusions

Together with favorable outcomes at the bone and kidney levels, potential negative impact of TAF on lipid profile should be included in the reflection to propose the most appropriate and tailored ARV treatment.
Efficacy of different nucleoside analog rescue therapies for entecavir-resistant chronic hepatitis B patients
Authors: Shang Jin , Zhou Juan , Liu Huan , Ise Rili M. , Tu You , Ran Jinqiu , Bai Lang , Tang Hong
Published: September 2021

Background

Entecavir (ETV) is recommended as a first-line anti-HBV treatment. However, many chronic hepatitis B patients initiate anti-HBV treatment such as lamivudine and telbivudine with low genetic barriers in China, which leads to compensatory mutations and increases the rate of ETV resistance. The management of ETV resistance in China is an essential clinical issue.

Methods

Patients from 2011 to 2017 with nucleos(t)ide analog resistance were screened and 72 patients with ETV resistance were included. These patients received different rescue therapies including an ETV and adefovir (ADV) combination therapy group (n = 25), a tenofovir (TDF) monotherapy group (n = 27), and an ETV and TDF combination therapy group (n = 20). Virologic, biochemical, and serologic responses were compared among the three groups.

Results

The rate of ETV resistance among all HBV-resistant variants increased from 6.04% in 2011 to 15.02% in 2017. TDF monotherapy and TDF combination groups showed similar rates of negative HBV DNA at 48 weeks (74.07% vs 70.00%, P > 0.05), while the ETV and ADV group showed the worst virologic response (28.00%). Also, TDF monotherapy and TDF combination therapy showed similar decline of HBV DNA at weeks 12, 24, and 48. There was no significant difference in the rates of HBeAg clearance, ALT normalization, and abnormal renal function among the three groups.

Conclusions

TDF monotherapy showed a comparable virologic response to TDF and ETV combination therapy and a better virologic response than ETV and ADV combination therapy. Thus, TDF monotherapy is the preferred rescue therapy for ETV resistance.
Perception of cure among leprosy patients post completion of multi-drug therapy
Authors: dos Santos Aleksandra Rosendo , de Souza Silva Pãmela Rodrigues , Costa Letícia Gomes , Steinmann Peter , Ignotti Eliane
Published: September 2021

Background

Leprosy is a treatable disease; however, the release from treatment after completion of multidrug therapy (MDT) often does not equal absence of health problems. Consequently, sequelae interfere with the patient’s perception of cure. The objective of this study was to analyze the factors associated with the perception of not being healed among people treated for leprosy in a highly endemic area in Brazil.

Method

A cross-sectional study of perceived cure of leprosy in the post-release from treatment period was conducted in Cáceres in the state of Mato Grosso, Brazil. The study included a total of 390 leprosy patients treated with MDT and released after completion of treatment from 1 January 2000 to 31 December 2017. The dependent variable was self-reported cure of leprosy; the independent variables included clinical, operational and socioeconomic variables.

Results

Out of the 390 former leprosy patients, 304 (77.9%) perceived themselves as cured and 86 (22.1%) considered themselves unhealed. Among the latter, 49 (57.0%) reported muscle weakness and joint pains. Individuals with complaints related to leprosy post-release from treatment had a 4.6 times higher chance to self-report as unhealed (OR 4.6; 95% CI 2.5–8.5). Patients with physical disabilities (PD) grade 1 and 2 at the time of the study had a 3.1 (OR 3.1; 95% CI 1.3–7.4) and 8.8 (OR 7.7; 95% CI 3.5–21.9) times higher likelihood to self-identify as unhealed, respectively.

Conclusion

Among successfully treated leprosy patients, a quarter self-report as unhealed of the disease. The factors associated with the perception of being unhealed are PD and complaints related to leprosy in the post-release from treatment phase.
Overexpression of UBE2C in esophageal squamous cell carcinoma tissues and molecular analysis
Authors: Li Rong , Pang Xing-Feng , Huang Zhi-Guang , Yang Li-Hua , Peng Zhi-Gang , Ma Jie , He Rong-Quan
Published: September 2021

Background

Esophageal cancer is a common malignant tumor and its 5-year survival rate is much lower than 30% due to its invasiveness and pronounced metastasis ability, as well as the difficulty in early diagnosis. This study aimed to elucidate the molecular mechanism of ubiquitin conjugating enzyme E2 C (UBE2C) in esophageal squamous cell carcinoma (ESCC).

Methods

In this study, we conducted a comprehensive evaluation of the UBE2C expression in ESCC by collecting the protein and mRNA expression data (including in-house RNA-seq, in-hosue immunohistochemistry, TCGA-GTEx RNA-seq and tissue microarray) to calculate a combined standardized mean difference (SMD) and summary receiver operating characteristic curve (sROC). Kaplan-Meier (K-M) method was used for survival analysis. We also explored the mechanism of UBE2C in ESCC by combing the differentially expressed genes (DEGs) of ESCC, related-genes of UBE2C in ESCC and the putative miRNAs and lncRNAs which may regulate UBE2C.

Results

UBE2C protein and mRNA were highly expressed in ESCC tissues (including 772 ESCC tissue samples and 1837 non-cancerous tissue control samples). The pooled SMD of UBE2C expression values was 1.98 (95% CI: 1.51–2.45, p < 0.001), and the the area under the curve (AUC) of the sROC was 0.93 (95% CI: 0.90–0.95). The results of survival analysis suggested that UBE2C is likely to play different roles in different stages of the ESCC. Pathway anaylsis showed that UBE2C mainly influenced the biological function of esophageal cancer by synergistic effects with CDK1, PTTG1 and SKP2. We also constructed a potential UBE2C-related ceRNA network for ESCC (HCP5/has-miR-139-5p/UBE2C).

Conclusion

UBE2C mRNA and protein level were highly expressed in ESCC and UBE2C was likely to play different roles in different stages of the ESCC.
Activation of quiescent polypoidal choroidal vasculopathy after membrane peeling vitrectomy for epiretinal membrane: a case report
Authors: Wang Yuelin , Chen Zhe , Yu Weihong , Chen Youxin
Published: September 2021

Background

Regular membrane peeling vitrectomy for epiretinal membrane (ERM) patients seldom causes large pigment epithelial detachment (PED). We presented an unusual case of the activation of quiescent polypoidal choroidal vasculopathy (PCV) after membrane peeling vitrectomy for ERM, with an uneven therapeutic process.

Case presentation

A 75-year-old female patient complained of metamorphopsia in her left eye for 2 years. Her best-corrected visual acuity was 20/160 with a moderate nuclear cataract. An irregular ERM and slight PED were shown in optical coherence tomography (OCT). No obvious orange-red lesion was detected. The patient underwent vitrectomy + ERM peeling + cataract surgery. After the operation, large PED emerged, and indocyanine green angiography (ICGA) confirmed PCV. Four monthly injections of intravitreal ranibizumab were administered, but PED persisted. After focal laser therapy targeted to the polyps combined with ranibizumab treatment, PED was absorbed.

Conclusions

Careful evaluation for PCV before membrane peeling vitrectomy for ERM is important, as indolent PCV may be activated postoperatively. Anti-VEGF therapy accompanied by laser photocoagulation may be more effective for PCV polyps located away from the fovea.
Relationship between chronic diseases and depression: the mediating effect of pain
Authors: Ma Ying , Xiang Qin , Yan Chaoyang , Liao Hui , Wang Jing
Published: September 2021

Background

Chronic diseases have a high incidence in China and may cause pain and depression. However, the association of chronic diseases with pain and the incidence of depression has not been comprehensively investigated.

Methods

The study population was obtained from the 2015 China Health and Retirement Longitudinal Study (CHARLS). The cross-sectional data from15,213 persons were included. CHARLS provides nationally representative data from21,097 individuals aged 45 years and older in approximately 150 districts and 450 villages. The main outcome was the incidence of depression. The main independent variable was chronic disease (no chronic disease, one chronic disease, and two or more chronic diseases). The mediators were the degree of pain (no pain, mild pain, and moderate to severe pain) and whether measures were taken to relieve pain (measures taken and no measures taken). We performed chi-square and binary logistic regression analyses of the associations of chronic disease with pain and the incidence of depression. The mediation model was examined using the Sobel test.

Results

Patients with more chronic diseases had more severe pain (OR = 3.697, P < 0.001, CI = 2.919–4.681) and were more likely to develop depression (OR = 2.777, P < 0.001, CI = 2.497–3.090). The degree of pain partially mediated the interaction between chronic disease and depression in this study (t = 7.989, P < 0.001). The incidence of depression was high in people who were female, less educated, unmarried, living in rural areas, and working.

Conclusions

The degree of pain had a partial mediating effect on chronic disease and depression. Pain relief measures should be considered when treating patients with depression.
Public attitudes to, and behaviours taken during, hot weather by vulnerable groups: results from a national survey in England
Authors: Erens Bob , Williams Lorraine , Exley Josephine , Ettelt Stefanie , Manacorda Tommaso , Hajat Shakoor , Mays Nicholas
Published: September 2021

Background

Hot weather leads to increased illness and deaths. The Heatwave Plan for England (HWP) aims to protect the population by raising awareness of the dangers of hot weather, especially for those most vulnerable. Individuals at increased risk to the effects of heat include older adults, particularly 75+, and those with specific chronic conditions, such as diabetes, respiratory and heart conditions. The HWP recommends specific protective actions which relate to five heat-health alert levels (levels 0–4). This study examines the attitudes to hot weather of adults in England, and the protective measures taken during a heatwave.

Methods

As part of a wider evaluation of the implementation and effects of the HWP, a survey (n = 3153) and focus groups, a form of group interview facilitated by a researcher, were carried out after the June 2017 level 3 heat-health alert. Survey respondents were categorised into three groups based on their age and health status: ‘vulnerable’ (aged 75+), ‘potentially vulnerable’ (aged 18–74 in poor health) and ‘not vulnerable’ (rest of the adult population) to hot weather. Multivariable logistic regression models identified factors associated with these groups taking protective measures. In-person group discussion, focused on heat-health, were carried out with 25 people, mostly aged 75 + .

Results

Most vulnerable and potentially vulnerable adults do not consider themselves at risk of hot weather and are unaware of the effectiveness of important protective behaviours. Only one-quarter of (potentially) vulnerable adults reported changing their behaviour as a result of hearing hot weather-related health advice during the level 3 alert period. Focus group findings showed many vulnerable adults were more concerned about the effects of the sun’s ultra-violet radiation on the skin than on the effects of hot temperatures on health.

Conclusions

Current public health messages appear to be insufficient, given the low level of (potentially) vulnerable adults changing their behaviour during hot weather. In the context of increasingly warmer summers in England due to climate change, public health messaging needs to convince (potentially) vulnerable adults of all the risks of hot weather (not just effects of sunlight on the skin) and of the importance of heat protective measures.