bluuebunny/crossref_metadata_2025 huggingface dataset:
Selective Loading and Prolonged Release of 5-Fluorouracil in the Nanoconfined Interlayer Space of Montmorillonite
Authors: Tan Daoyong , Li Yan , Zhang Zheng , Sun Shiyong , Sun Hongjuan , Li Bowen
Published: November 2020

Montmorillonite was used as a carrier for the anticancer drug 5-fluorouracil (5FU). The selective loading of 5FU into the nanoconfined interlayer space of montmorillonite was achieved by rinsing off the weakly bonded 5FU from the external surface. The 5FU loading content in montmorillonite
was 3.2 mass%. The intercalated 5FU was in an amorphous state and might be arranged as a roughly vertical monolayer in the interlayer space of montmorillonite. The intercalated 5FU showed a high thermal stability due to the protection of the montmorillonite layers. The release profiles of
the intercalated 5FU were well fitted with the modified Korsmeyer-Peppas model. The montmorillonite exhibited a prolonged release of 5FU due to the restriction of the outward diffusion of intercalated 5FU. The 5FU/montmorillonite system has promising potential for oral administration for colonspecific
delivery.
Bosoite, a new silica clathrate mineral from Chiba Prefecture, Japan
Authors: Momma Koichi , Ikeda Takuji , Nagase Toshiro , Kuribayashi Takahiro , Honma Chibune , Nishikubo Katsumi , Takahashi Naoki , Takada Masayuki , Matsushita Yoshitaka , Miyawaki Ritsuro , Matsubara Satoshi
Published: November 2020

Bosoite (IMA2014-023) is a new silica clathrate mineral containing hydrocarbon molecules in its crystal structure. Bosoite can be considered structurally as a silica analogue of the structure-H gas hydrate, where guest molecules are trapped in cage-like voids constructed of the host framework. The mineral occurs in the Miocene tuffaceous sedimentary rocks at Arakawa, Minami-boso City, Chiba Prefecture, Japan. Bosoite is hexagonal, and it crystallises as an epitaxial intergrowth on chibaite crystals, with the {0001} of bosoite parallel to octahedral {111} form of chibaite. Crystals are colourless and transparent with vitreous lustre. The calculated density is 2.04 g/cm3. The empirical formula (based on 2 O apfu and guest molecules assumed as CH4) is Na0.01(Si0.98Al0.02)Σ1.00O2⋅0.50CH4; the end-member formula is SiO2⋅nCxH2x+2. Bosoite has the space groupP6/mmm, with the unit-cell parametersa= 13.9020(3) Å,c= 11.2802(2) Å,V= 1887.99(6) Å3andZ= 34. The crystal structure of bosoite was refined by single-crystal X-ray diffraction and converged toR1= 4.26% for the average model andR1= 2.96% for the model where all oxygen sites are split.
Safety of measles, mumps, and rubella vaccine in egg allergy: in vivo and in vitro management
Authors: Magistà Stefania , Albanesi Marcello , Chaoul Nada , Di Bona Danilo , Di Leo Elisabetta , Nettis Eustachio , Caiaffa Maria Filomena , Macchia Luigi
Published: November 2020

Background

Egg allergy is the second most prevalent form of food allergy in childhood. In spite of the evidence accumulated, inoculating egg allergy children with attenuated vaccines grown on chick embryo cell cultures, such as the measles, mumps, and rubella (MMR) vaccine, is regarded (erroneously) as potentially dangerous or even anaphylactogenic, by many. An issue perceived as particularly conflicting also by Health Professionals.

Case presentation

A 15-year-old boy, with a history of severe egg allergy in early infancy, who was still sensitized to egg allergens, including baked egg, had never received MMR vaccination, in fear of possible anaphylaxis, in spite of the fact that this vaccination is mandatory in the first year of life, in Italy. Because of that, he was not allowed to attend school, longer, and was referred to us in order to assess the potential risk of MMR vaccination. Upon thorough allergologic workup, sensitization to MMR vaccine components was excluded by an in vivo approach, consisting in skin prick tests, intradermal tests, and subcutaneous injection test, corroborated by vaccine-specific B-lymphocyte proliferation assay, ex vivo. T-cell proliferation in response to MMR vaccine was also excluded. Eventually, the boy was inoculated with MMR vaccine and was readmitted to school.

Conclusions

The diagnostic strategy adopted appears feasible and easy-to-perform and may be adopted in controversial cases (as the one reported), characterized by previous severe allergic reactions to egg. The B-lymphocyte proliferation assay we developed may represent a useful and reliable tool not only in research but also in clinical practice.
Anesthetic management of a 12-year-old child with Kagami-Ogata syndrome for pectus excavatum: a case report
Authors: Nishimoto Hisako , Yagihara Masahiro , Uemura Aki , Nakajima Yoshiki
Published: November 2020

Background

Kagami-Ogata syndrome (KOS) is due to abnormal gene expression in the 14q32.2 imprinted region. Laryngomalacia and bell-shaped thorax of children with KOS can affect airway management of general anesthesia.

Case presentation

A 12-year-old girl with KOS had a mechanical ventilation history and underwent pectus excavatum repair for cosmetic reasons. Although she had undergone invasive thoracic surgery under general and epidural anesthesia, her respiratory rate and tidal volume were stable with adequate pain control mainly through epidural analgesia at the end of the surgery. We examined her larynx by a bronchoscope. Then, we successfully extubated her after confirming the normal movement of her larynx.

Conclusions

When patients with KOS undergo pectus excavatum repair, anesthesiologists should prevent postoperative respiratory failure by providing adequate postoperative analgesia. Evaluation of airway patency and respiratory pattern before extubation is critical.
Machine learning predictivity applied to consumer creditworthiness
Authors: Aniceto Maisa Cardoso , Barboza Flavio , Kimura Herbert
Published: November 2020

Credit risk evaluation has a relevant role to financial institutions, since lending may result in real and immediate losses. In particular, default prediction is one of the most challenging activities for managing credit risk. This study analyzes the adequacy of borrower’s classification models using a Brazilian bank’s loan database, and exploring machine learning techniques. We develop Support Vector Machine, Decision Trees, Bagging, AdaBoost and Random Forest models, and compare their predictive accuracy with a benchmark based on a Logistic Regression model. Comparisons are analyzed based on usual classification performance metrics. Our results show that Random Forest and Adaboost perform better when compared to other models. Moreover, Support Vector Machine models show poor performance using both linear and nonlinear kernels. Our findings suggest that there are value creating opportunities for banks to improve default prediction models by exploring machine learning techniques.
Relationships between the Religious Backgrounds and Evolution Acceptance of Black and Hispanic Biology Students
Authors: Barnes M. Elizabeth , Supriya K. , Dunlop Hayley M. , Hendrix Taija M. , Sinatra Gale M. , Brownell Sara E.
Published: November 2020

An evolution education study spanning 77 courses and 17 states found that Black and Hispanic students’ stronger than average religiosity explains their lower evolution acceptance. This indicates a need to take into account students’ religious culture and background to teach evolution in an inclusive way.
Improving Pediatric Neuro-Oncology Survival Disparities in the United States–Mexico Border Region: A Cross-Border Initiative Between San Diego, California, and Tijuana, Mexico
Authors: Aristizabal Paula , Burns Luke P. , Kumar Nikhil V. , Perdomo Bianca P. , Rivera-Gomez Rebeca , Ornelas Mario A. , Gonda David , Malicki Denise , Thornburg Courtney D. , Roberts William , Levy Michael L. , Crawford John R.
Published: November 2020

PURPOSE

Treatment of children with CNS tumors (CNSTs) demands a complex, interdisciplinary approach that is rarely available in low- and middle-income countries. We established the Cross-Border Neuro-Oncology Program (CBNP) between Rady Children’s Hospital, San Diego (RCHSD), and Hospital General, Tijuana (HGT), Mexico, to provide access to neuro-oncology care, including neurosurgic services, for children with CNSTs diagnosed at HGT. Our purpose was to assess the feasibility of the CBNP across the United States-Mexico border and improve survival for children with CNSTs at HGT by implementing the CBNP.

PATIENTS AND METHODS

We prospectively assessed clinicopathologic profiles, the extent of resection, progression-free survival, and overall survival (OS) in children with CNSTs at HGT from 2010 to 2017.

RESULTS

Sixty patients with CNSTs participated in the CBNP during the study period. The most common diagnoses were low-grade glioma (24.5%) and medulloblastoma (22.4%). Of patients who were eligible for surgery, 49 underwent resection at RCHSD and returned to HGT for collaborative management. Gross total resection was achieved in 78% of cases at RCHSD compared with 0% at HGT ( P < .001) and was a predictor of 5-year OS (hazard ratio, 0.250; 95% CI, 0.067 to 0.934; P = .024). Five-year OS improved from 0% before 2010 to 52% in 2017.

CONCLUSION

The CBNP facilitated access to complex neuro-oncology care for underserved children in Mexico through binational exchanges of resources and expertise. Survival for patients in the CBNP dramatically improved. Gross total resection at RCHSD was associated with higher OS, highlighting the critical role of experienced neurosurgeons in the treatment of CNSTs. The CBNP model offers an attractive alternative for children with CNSTs in low- and middle-income countries who require complex neuro-oncology care, particularly those in close proximity to institutions in high-income countries with extensive neuro-oncology expertise.
Mammary development in the embryo and adult: new insights into the journey of morphogenesis and commitment
Authors: Watson Christine J. , Khaled Walid T.
Published: November 2020

The mammary gland is a unique tissue and the defining feature of the class Mammalia. It is a late-evolving epidermal appendage that has the primary function of providing nutrition for the young, although recent studies have highlighted additional benefits of milk including the provision of passive immunity and a microbiome and, in humans, the psychosocial benefits of breastfeeding. In this Review, we outline the various stages of mammary gland development in the mouse, with a particular focus on lineage specification and the new insights that have been gained by the application of recent technological advances in imaging in both real-time and three-dimensions, and in single cell RNA sequencing. These studies have revealed the complexity of subpopulations of cells that contribute to the mammary stem and progenitor cell hierarchy and we suggest a new terminology to distinguish these cells.
Regulatory mechanisms governing epidermal stem cell function during development and homeostasis
Authors: Flora Pooja , Ezhkova Elena
Published: November 2020

Cell divisions and cell-fate decisions require stringent regulation for proper tissue development and homeostasis. The mammalian epidermis is a highly organized tissue structure that is sustained by epidermal stem cells (ESCs) that balance self-renewal and cell-fate decisions to establish a protective barrier, while replacing dying cells during homeostasis and in response to injury. Extensive work over past decades has provided insights into the regulatory mechanisms that control ESC specification, self-renewal and maintenance during different stages of the lifetime of an organism. In this Review, we discuss recent findings that have furthered our understanding of key regulatory features that allow ESCs to establish a functional barrier during development and to maintain tissue homeostasis in adults.
A Quantitative and Qualitative Analysis of Changes during Systemic Family Therapy:
Results of the Polish Clinical Version of the SCORE–15 Questionnaire
Authors: Matusiak Feliks , Wolska Małgorzata , Ulasińska Roma , Stratton Peter , Józefik Barbara
Published: November 2020

Cel pracy

W artykule zaprezentowano rezultaty projektu badawczego poświęconego ocenie zmian w procesie systemowej terapii rodzinnej prowadzonej w grupie klinicznej młodzieżowych pacjentów psychiatrycznych.

Metoda

Do oceny wykorzystano polską wersję kwestionariusza SCORE–15. Badanie przeprowadzono na grupie 109 rodzin, które wypełniły kwestionariusz przed pierwszą sesją. Przed czwartą sesją ankietę wypełniły 73 rodziny; a po ostatniej sesji ukończyło ją 28 rodzin.

Wyniki

Wyniki konsensualnych analiz jakościowych ujawniły zmiany w opisie rodziny, a także w opisie problemu. Analizy statystyczne wyników kwestionariusza SCORE–15 wykazały znaczące zmiany między pierwszą, czwartą i ostatnią sesją. Zmiany zostały zidentyfikowane w skali SCORE Total, a także w skalach VAS. Wskaźnik RCI wykazał 5 popraw i 2 pogorszenia po trzech sesjach oraz brak pogorszeń i 13 popraw pod koniec procesu terapii.

Wnioski

Uzyskane wyniki wskazują na wiele istotnych zmian w badanych obszarach po przejściu rodzin przez systemową terapię. Badanie wykazało także, że spójna analiza jakościowa materiału opisowego może być bardzo użyteczna i może wzbogacić zarówno rozumienie procesu terapeutycznego, jak i sposób przekazywania rodzinom informacji o zmianach w ramach terapii.
ECG of the month
Authors: Regan Martin
Published: November 2020

In this issue's ECG of the month, Martin Regan presents a case of a 56-year-old man with a thoracotomy wound following an aortic valve replacement.
Reflections of research: united colours of myocytes
Authors: Foldes Gabor , Talman Virpi
Published: November 2020

The annual image competition, Reflections of Research, provides a glimpse into the cutting-edge heart and circulatory research funded by the British Heart Foundation
New technique for atrial fibrillation ablation
Authors: Palmer Sarah Jane
Published: November 2020

Sarah Jane Palmer discusses the latest research in technology.
Diet composition and neighboring prey community of the Phuping newt (Tylototriton uyenoi) in Maesa–Kogma Biosphere Reserve, Chiang Mai Province, northern Thailand
Authors: DOWWIANGKAN THANSUDA , CHUAYNKERN YODCHAIY , DUMRONGROJWATTANA PONGRAT , Duengkae Prateep
Published: November 2020

Abstract. Dowwiangkan T, Chuaynkern Y, Dumrongrojwattana P, Duengkae P. 2020. Diet composition and neighboring prey community of the Phuping newt (Tylototriton uyenoi) in Maesa–Kogma Biosphere Reserve, Chiang Mai Province, northern Thailand. Biodiversitas 21: 4515-4523. The present work reports the comparison of fecal pellet prey composition of Tylototriton uyenoi from Maesa-Kogma Biosphere Reserve (Chiang Mai Province, northern Thailand) based on an analysis of its fecal pellets. Males of T. uyenoi consumed more diverse prey than those of females (53 groups of prey versus 14 groups). The analysis reveals a diverse diet of Arthropoda (Insecta, Arachnida, Diplopoda, Chilopoda, and Malacostraca), Mollusca (Gastropoda), Chordata, and conspecific eggs. Partial remnants of plant materials were also found in the pellets. The percentage frequency of prey occurrence was higher in Formicidae (21.34), Lymnaeidae (17.39), unknown snails (10.67), and Blattidae (9.881). The percentage of the relative number of preys was higher in Lymnaeidae (41.02) and Formicidae (17.17). The volumetric prey percentage was higher in Lymnaeidae (30.78), Paradoxosomatidae (19.14), and unknown snails (12.71). The index of relative importance was higher in Lymnaeidae (1,248.62), Formicidae (376.972), unknown snails (188.275), and Paradoxosomatidae (166.433). The percentage of IRI was higher in Lymnaeidae (57.128) and Formicidae (17.248). The Spearman rank coefficient (95% confidence interval test) showed a significant positive correlation (p<0.05) between prey volume and number and SVL.
The practice and plants used in Besale ritual healing by the Anak Dalam Tribe in Nyogan Village, Jambi, Indonesia
Authors: Asra Revis , Marina Silalahi  , FIJRIDIYANTO IZU ANDRY
Published: November 2020

Abstract. Asra R, Silalahi M, Fijridiyanto IA. 2020. The practice and plants used in Besale ritual healing by the Anak Dalam Tribe in Nyogan Village, Jambi, Indonesia. Biodiversitas 21: 4529-4536. The Anak Dalam Tribe (ADT) is an indigenous ethnic which inhabits the center of Sumatra, especially in Jambi and South Sumatra Provinces, Indonesia. The ADT communities still maintain their traditional rituals; one of them is the Besale ritual healing (BRH). This study aims to analyze the diversity of plants used in BRH by the ADT in Nyogan Village, Jambi Province, Indonesia. The study was conducted with an ethnobotany approach through surveys using interviews and participatory observations. The total number of informants was six persons, namely the leaders and performers of the BRH. The data were analyzed qualitatively using descriptive statistics. The BRH is carried out by a dukunorsidi (shaman), bujang pembayun (dancers), biduan (singers), and inang (assistant). They combine ritual and treatment simultaneously to cure people who have severe illnesses. There are 21 species belong to 20 genera and 12 families of plants used in the BRH. The plants are used to build a hall (place for the BRH), "bird" ornament, and offerings. The most widely used part of the plant is the flowers (34,61%). The plants used in the BRH are fresh materials, which are burned, smoked, hung, and eaten during the ritual.
Response of parasitoids to invasive pest Phenacoccus manihoti Matile-Ferrero (Hemiptera: Pseudococcidae) on cassava crop in Bali, Indonesia
Authors: SUPARTHA I WAYAN , YUDHA I KADEK WISMA , WIRADANA PUTU ANGGA , SUSILA I WAYAN
Published: November 2020

Abstract. Supartha IW, Yudha IKW, Wiradana PA, Susila IW. 2020. Response of parasitoids to invasive pest Phenacoccus manihoti Matile-Ferrero (Hemiptera: Pseudococcidae) on cassava crop in Bali, Indonesia. Biodiversitas 21: 4543-4549. Phenacoccus manihoti Matile-Ferrero (Hemiptera: Pseudococcidae) is an invasive pest that entered Indonesia in 2010 and attacks cassava plants, especially in Bali Province. This study aimed to identify species and establish the population distribution mapping, community structures (diversity, abundance, and dominance), and parasitization rate on cassava crops in Bali. This study conducted with a field observation method by taking infested shoots of cassava plants by P. manihoti purposively in the field. The cassava mealybug that was present in the leaves or shoots were kept in the laboratory until the parasitoid appeared. The results showed that four species of parasitoids were found to respond to invasive pest, namely Anagyrus lopezi (Encyrtidae), Acerophagus sp. (Encyrtidae), Blepyrus sp. (Encyrtidae) and Encarsia sp. (Aphelinidae). Structurally the parasitoid community had a low abundance (R1 = 0.50–0.87) and low diversity index value (H'= 0.27-0.28). While the parasitoid dominance index was in the moderate category with a value D = 0.33–0.59 in Bangli, Klungkung, Badung, Tabanan, and Buleleng Districts. But it had high dominance index values in Gianyar, Denpasar, Jembrana, and Karangasem Districts with a value D = 0.62-0.72. The three species of parasitoid spread evenly in all districts and cities in Bali according to the host distribution map, namely P. manihoti on cassava crop. The four parasitoids showed varying parasitic levels in the field with the highest parasitization rate was A. lopezi followed by Acerophagus sp., Blepyrus sp., and Encarsia sp.
Phenotypic plasticity of eddoe and dasheen taro genotypes in response to saturated water and dryland cultivations
Authors: Hidayatullah Careca Sepdihan Rahmat , Santosa Edi , Sopandie Didy , Hartono Arief
Published: November 2020

Abstract. Hidayatullah CSR, Santosa E, Sopandie D, Hartono A. 2020. Phenotypic plasticity of eddoe and dasheen taro genotypes in response to saturated water and dryland cultivations. Biodiversitas 21: 4550-4557.  The phenotypic plasticity of dasheen and eddoe taro genotypes was evaluated based on growth and yield characters to select proper genotypes in response to climate change. The study was conducted at the Leuwikopo Experimental Farm, Bogor, Indonesia from May to October 2019. Dasheen (Talas Sutra and Talas Bentul) and eddoe genotypes (S28 and S19) were planted in saturated water cultivation (SWC) and dryland cultivation (DC). DC relied on rainwater, and SWC was manipulated dryland by flooding. SWC promoted vigorous growth and tuber weight, irrespective of genotypes. Increasing taro biomass production in SWC was supported by higher photosynthetic rate, leaf number, and size. Dasheen genotypes produced higher tuber weight than the eddoe in SWC, conversely, the eddoe tended to produced heavier tuber than the dasheen in DC; indicating phenotypic plasticity is strongly affected by soil moisture and genotype. The dasheen had more plastic growth and yield characters to soil moisture than the eddoe genotypes, with plasticity level, ranging from low to very high. Taro had high resiliency to multiple abiotic stresses, e.g. flood, and drought. Considering the marketable value of the yield, dasheen and eddoe genotypes are recommended in flooding and drought-prone areas, respectively.
Seed germination characteristics in different storage time of Gmelina arborea treated with ultrafine bubbles priming
Authors: SIREGAR ISKANDAR Z. , MUHARAM KARIMA FAUZIAH , PURWANTO Y. ARIS , SUDRAJAT DEDE J.
Published: November 2020

Abstract. Siregar IZ, Muharam KF, Purwanto YA, Sudrajat DJ. 2020. Seed germination characteristics in different storage time of Gmelina arborea treated with ultrafine bubbles priming. Biodiversitas 21: 4558-4564.  Gmelina (Gmelina arborea) seed collected from tropical Indonesian forest experience seed deterioration during storage which is relatively faster than the gmelina seeds originating from temperate regions, such as India and Myanmar. Various treatments have been made to improve the seed viability and vigor after storage through various invigoration techniques. However, the utilization of promising novel technology such as ultrafine bubbles (UFB) has not been evaluated yet. The objective of this study was to determine the effect of seed priming using UFB, polyethylene glycol (PEG), and gibberellic acid (GA3) on the viability and vigor of gmelina seeds that have been stored for one and two years. This study was conducted by employing Completely Randomized Design (CRD) using three replicates. The study showed priming treatment had significant effect and formed average germination capacity of 80% for the seed stored for 1 year.  On the other hand, the priming treatment using PEG-0.8 MPa was the best treatment for germination capacity of 74.67% on 2 years stored seeds. UFB had significant effect on germination capacity, germination rate, growth uniformity, and germination value. This result was expected due to reactive oxygen species (ROS) produced by micro-nano bubbles that could increase physiological activity of the seed cells.
Genetic structure of the Capoeta aculeata populations inferred from microsatellite DNA loci
Authors: Gandomkar Habibollah , Hosseini Shekarabi Seyed Pezhman , Abdolhay Hossein Ali , Nazari Sajad , MEHRJAN MEHDI SHAMSAEI
Published: November 2020

Abstract. Gandomkar H, Shekarabi SPH, Abdolhay HA, Nazari S, Mehrjan MS. 2020. Genetic structure of the Capoeta aculeata populations inferred from microsatellite DNA loci. Biodiversitas 21: 4565-4570. The present study aimed to investigate the genetic variation of Capoeta aculeata on the basis of DNA microsatellite loci from three rivers (Beshar, Khersan, Maroun) in Kohgiluyeh and Boyer-Ahmad Province in Iran. DNA from fin clips of 120 specimens extracted and was examined with eight microsatellite markers. Genetic differences between the populations were discerned by pairwise comparison based on allelic distribution. The average number of alleles per locus ranged from 4 to 14, while the average observed heterozygosity (Ho) at various loci varied between 0.212 to 0.579, implying that a moderate level of genetic variation. Among three populations, Maroun River population displayed the highest level of variability in terms of heterozygosity. Tests of Hardy-Weinberg showed that the microsatellite loci deviated significantly in the populations. The results indicate that some of the populations were significantly differentiated from one another based on pairwise FST estimates. Genetic distance-based measures supported the clustering of Maroun, Beshar, and Khersan rivers. The neighbor-joining dendrogram topology constructed on the basis of genetic distances among populations supported observed division between the populations. The non-significant differentiation between C. aculeata samples from Beshar and Khersan can be explained by a relative disconnection of these two populations and/or small amounts of gene flow.
Morphometric and genetic variations of species composers of nike fish assemblages in Gorontalo Bay Waters, Indonesia
Authors: Sahami Femy M. , Kepel Rene Charles , Olii Abdul Hafidz , Pratasik Silvester Benny , Lasabuda Ridwan , Wantasen Adnan , Habibie Sitty Ainsyah
Published: November 2020

Abstract. Sahami FM, Kepel RC, Olii AH, Pratasik SB, Lasabuda R, Wantasen A, Habibie SA. 2020. Morphometric and genetic variations of species composers of nike fish assemblages in Gorontalo Bay Waters, Indonesia. Biodiversitas 21: 4571-4581.  Nike is Gobioidei fish at the post-larval and juvenile stages whose habitat is still in seawater before they migrate to the freshwater, grow up, and spawn to fulfill their life cycle as an amphidromous species. This study aims to identify the species composers of Nike fish in Gorontalo Bay waters based on their morphometric and molecular characters. 2,523 samples were collected from the catches of fishermen during three periods of their appearance in Gorontalo Bay waters from January to March 2019. The samples were grouped based on their similarity of melanophore patterns, and morphometric characters of 10 units were then measured. This study found 20 different groups of melanophore patterns; 15 of them were new melanophore patterns. DNA samples from each group of new melanophore patterns were then isolated for molecular analysis. The morphometric analysis grouped the 20 melanophore patterns into three separate clusters that were confirmed through molecular analysis. The results of Gen Cytochrome Oxidase I (COI) sequences indicate that the new melanophore patterns of Nike fish assemblages consisting of six species; five species in the Gobiidae family (i.e. Sicyopterus parvei, S. cynocephalus, S. longifilis, S. lagocephalus, and Stiphodon semoni) and a species in the Eleotridae family (Belobranchus belobranchus).
Heavy metals contaminants in the eggs and temperatures of nesting beaches of sea turtles in Kaimana, West Papua, Indonesia
Authors: Tapilatu Ricardo F , Wona Hengki , Siburian Rima , SALEDA SEFRIANTO T.
Published: November 2020

Abstract. Tapilatu RF, Wona H, Siburian RHS, Saleda ST. 2020. Heavy metals contaminants in the eggs and temperatures of nesting beaches of sea turtles in Kaimana, West Papua, Indonesia. Biodiversitas 21: 4582-4590. Etna Bay and Venu Island in Kaimana, West Papua, Indonesia are two of many areas that sea turtles use during the nesting season. Here, we report data on heavy metals contaminants from a sample of eggs of green (Chelonia mydas) and hawksbill (Eretmochelys imbricata) turtles collected from a subset of two nests during the 2016 nesting season at Venu Island, Kaimana, West Papua, Indonesia. Three heavy metals contaminants (i.e. mercury, cadmium, arsenic) found in eggs exceeded the established safety limits for human consumption. Other contaminants such as lead, zinc, manganese, iron, and copper were found below the established safety standards. There is an implication of the containment of heavy metals in sea turtle eggs to human health in Kaimana when it is consumed. It is highly recommended that the harvesting of turtle eggs and adults of all of sea turtles at Kaimana be totally prohibited. In addition, overall, beach temperatures in Lakahia fluctuated at a larger range between 26.5o and 33.8oC than at Venu island that fluctuated between 25.9o and 30.2oC. Mean daily temperatures of in-situ nests during the middle third of the incubation period were above the pivotal temperature of 29.0oC for temperature-dependent sex determination suggesting a female bias may already exist. The chemical contamination found in the eggs of C. mydas and E. imbricata at Venu Island is thought to assist sea turtle conservation initiatives in the area. The conservation program at the prime nesting habitat for green and hawksbill turtles on the small islands west of Kaimana such as Venu Island should be intensified to reverse the decline and increase population size of nesting sea turtle species. It is recommended that education and outreach be implemented in surrounding communities in Kaimana to raise awareness about marine turtle conservation.
The quality of fermented goat milk produced by Pediococcus acidilactici BK01 on refrigerator temperature
Authors: Melia Sri , Juliyarsi Indri , Fitri Kurnia Yulianti , Endra Pratama Yudha , Rezzy Pratama Dhiva
Published: November 2020

Abstract. Melia S, Juliyarsi I, Kurnia YF, Pratama YE, Pratama DR. 2020. The quality of fermented goat milk produced by Pediococcus acidilactici BK01 on refrigerator temperature. Biodiversitas 21: 4591-4596. This research aimed to determine the quality of chemical and microbiological milk of goats fermented with Pediococcus acidilactici BK01 with the storage time on refrigerator temperature. The method used is the experimental method of Completely Randomized Design with five times the treatment of goat milk fermentation at the refrigerator temperature is over 0, 7, 14, 21, and 28 days with four repetitions. The results showed long-lasting storage of real effect (P < 0.05) on water content, pH, titratable acid, total lactic acid bacteria, and total plate count, but no noticeable effect (P > 0.05) on the protein levels and milk fat fermentation during the resulting storage. The value of moisture content during storage ranges between 85.88-84.92%, pH 4.48-4.28 , 3.69-3.49% fat content, 3.53-3.58% protein content, as well as the count of titratable acid ranging from 1.52-1.73%. The whole colonies of lactic acid bacteria reached between 9.106 log CFU/mL to 10.376 log CFU/mL, and the total plate count (TPC) value during storage experienced an increase from 2.653 log CFU/mL to 4.012 log CFU/mL. Based on the results of the study can be concluded that the milk of goat fermentation with P. acidilactici BK01 can maintain quality until the retention period of 28 days with the viability of lactic acid bacteria that meet the category as probiotics and still liked by the consumer.
Chemical compounds contained in young and mature leaves of agarwood species Wikstroemia tenuiramis and its antioxidant properties
Authors: BATUBARA RIDWANTI , HANUM TENGKU ISMANELLY , AFFANDI ODING , WAHYUNI HENNY SRI
Published: November 2020

Abstract. Batubara R, Hanum TI, Affandi O, Wahyuni HS. 2020. Chemical compounds contained in young and mature leaves of agarwood species Wikstroemia tenuiramis and its antioxidant properties. Biodiversitas 21: 4616-4622. The genus Wikstroemia is one of the genus of agarwood producing trees which has not been widely researched and published. One type of this genus is Wikstroemia tenuiramis Miq, which grows naturally in North Sumatra, the leaves are like other types of agarwood leaves can be used as raw material for agarwood leaves tea. A research has been conducted to determine the chemical contents contained in the young and mature leaves of agarwood plants species W. Tenuiramis. This research methods applied qualitative analysis using phytochemical screening, quantitative analysis to determine tannin content using titration method, antioxidant activity analysis using 2,2-diphenil-1-picrylhydrazil (DPPH) method, and compound tracing using Py-gas chromatography-mass spectrometry (Py-GC-MS) instruments. The qualitative phytochemical analysis results showed no difference in chemical compounds contained in both the young and mature leaves. Both the young and mature leaves contained flavonoids, triterpenoids, and tannins, and did not contain alkaloids, steroids and saponins. The young contained tannins of 1.079 ± 0.001% and the mature contained tannins of 4.645 ± 0.021%, not significant statistically. Py-GC-MS analysis showed the presence of various 30 bioactive compounds contained in both young and mature leaves. The analysis results also showed that both the young and mature leaves had very strong antioxidant activity. Our findings suggest that the two types of leaves have equal utility value of in term of chemical contents and antioxidant potentials.
Diversity and honey properties of stingless bees from meliponiculture in East and North Kalimantan, Indonesia
Authors: SYAFRIZAL  , RAMADHAN RICO , WIJAYA KUSUMA IRAWAN , EGRA SAAT , SHIMIZU KUNIYOSHI , KANZAKI MAMORU , Tangkearung Enos
Published: November 2020

Abstract. Syafrizal, Ramadhan R, Kusuma IW, Egra S, Shimizu K, Kanzaki M, Arung ET. 2020. Diversity and honey properties of stingless bees from meliponiculture in East and North Kalimantan, Indonesia. Biodiversitas 21: 4623-4630. Kalimantan Island in Indonesia is covered by tropical rain forests that are rich in biodiversity. Species, include a stingless bee (Trigona spp.). Recently, stingless bee beekeeping (Meliponiculture) in this region has become popular because the honey has a unique taste, a mix of sweet, sour, and bitter which is highly valued. The objective of this study was to determine the diversity of stingless bee species, the number of colonies, type of hive, and honey properties such as taste, acidity, color, phytochemicals, and radical scavenging activity or antioxidant in meliponiculture in selected areas of Eastern and Northern Kalimantan, Indonesia. The area studied included Samarinda, Balikpapan, Penajam, Bontang, Sangatta, and Tarakan. The study conducted by visiting the farmers, collecting the stingless bees species and bee products (honey, propolis, and bee pollen), pH, color and taste determination, phytochemical screening, and DPPH assay. We found 12 species, with Heterotrigona itama and Tetragonula laeviceps being the main ones cultivated by people in all research areas, except Balikpapan. We counted 111 colonies of T. laeviceps and 99 of H. itama in the meliponiculture. There were three types of hive used by farmers in meliponiculture. Most of the honey tasted sweet and sour with acidity or pH ranging from 3-4, and color from dark brown to light yellow. The phytochemicals contained tannin, alkaloid, flavonoid, triterpenoid, carotenoid, coumarin, saponin, and carbohydrate. The Tetragonula sarawakensis honey was the most potent in term radical scavengers. Our findings showed that stingless bee honey contains antioxidants with phytochemicals that are very useful for human health.
Short Communication: Pattern of antibiotic resistance on extended-spectrum beta-lactamases genes producing Escherichia coli on laying hens in Blitar, Indonesia
Authors: WIBISONO FRESHINTA JELLIA , SUMIARTO BAMBANG , UNTARI TRI , Effendi Mustofa Helmi , PERMATASARI DIAN AYU , WITANINGRUM ADIANA MUTAMSARI
Published: November 2020

Abstract. Wibisono FJ, Sumiarto B, Untari T, Effendi MH, Permatasari DA, Witaningrum AM. 2020. Short Communication: Pattern of antibiotic resistance on extended-spectrum beta-lactamases genes producing Escherichia coli on laying hens in Blitar, Indonesia. Biodiversitas 21: 4631-4635. The aims of this study were to determine the susceptibility pattern of phenotypic antibiotics on extended-spectrum beta-lactamases (ESBL) genes and genotype profiles of ESBL producing Escherichia coli strains isolated from cloacal samples of laying hens in Blitar. A total of 165 cloacal swab samples were successfully isolated 145 E. coli strains during the study taken from 5 subdistricts in Blitar. All the strains were examined for antibiotic resistance patterns by disk diffusion method with double-disk synergy test (DDST), followed testing with VITEK® 2 methods, molecular identification of ESBL coding genes using PCR. The results of this study showed that the characterization of nucleotide analysis from PCR amplification of ESBL-producing E. coli bacteria isolated from laying hens in Blitar showed that eight isolates were the dominant of CTX gene, followed by the TEM encoding gene of two isolates, and the SHV coding gene as much as one isolate. The presence of more than 1 encoding genes in the E. coli bacterial isolate was seen in 1 isolate, where the isolate carried the CTX gene and the SHV gene as well. All ESBL producing E. coli isolates were resistant to amoxicillin, ampicillin, cefazolin, cefotaxime, and ceftriaxone, and these ESBL isolates were more than 70% resistant to gentamicin, aztreonam, and trimethoprim/sulfamethoxazole. These results indicated that poultry is a potential reservoir for ESBL-producing E. coli. The presence of ESBL-producing E. coli in poultry requires strengthening antibiotic policy. This is important because the regulation of antibiotic use in poultry is gaining momentum to increase animal productivity and food safety in Blitar, Indonesia.
Diversity and distribution of mollusks at three zones of mangrove in Pejarakan, Bali, Indonesia
Authors: GINANTRA I KETUT , MUKSIN I KETUT , SUASKARA IDA BAGUS MADE , JONI MARTIN
Published: November 2020

Abstract. Ginantra IK, Muksin IK, Suaskara IBM, Joni M. 2020. Diversity and distribution of mollusks at three zones of mangrove in Pejarakan, Bali, Indonesia. Biodiversitas 21: 4636-4643. The diversity of mangrove species in mangrove zonations on the coast of Pejarakan plays an important role in the existence of faunal diversity, including those from mollusks. Mollusks are the dominant phylum associated with mangrove habitats, in addition to the species of the Crustacean subphylum. This research aimed to determine the diversity of mollusks and their distribution patterns in three mangrove zones in Pejarakan, Buleleng District, Bali Province, Indonesia. Data collection was conducted by establishing sampling plots across three mangrove zones, i.e. seaward zone, middle zone, and landward zone. There were 23 sampling plots in total with size of each plot was 1x1m. The species and number of individuals of each species of mollusks were recorded in each quadrat plot. The diversity of mollusks was calculated using the Shannon-Wiener index, evenness index, and dominance index, while the pattern of distribution of mollusks was measured using the Morisita index. The results of the study found 27 species of mollusks, consisting of 19 species from Gastropods group, 7 species from Bivalvia group and 1 species from Polyplacophora (chiton). The seaward zone showed the highest species diversity, while the landward zone had the lowest. Terebralia palustris had the highest abundance with 217 individuals/m2 and was distributed in the three mangrove zones. Planaxis sulcatus showed the highest abundance (87 ind./m2) in the seaward zone, and Telescopium telescopium showed a fairly high abundance and were distributed in the mid zone and the landward zone. In general, mollusks in the mangrove area of Pejarakan was distributed in a group/clustered pattern. The data from this research can serve as a reference in mangrove forest conservation efforts and mangrove forest used for ecotourism.
Detection and prevalence of multidrug-resistant Klebsiella pneumoniae strains isolated from poultry farms in Blitar, Indonesia
Authors: PERMATASARI DIAN AYU , WITANINGRUM ADIANA MUTAMSARI , WIBISONO FRESHINTA JELLIA , Effendi Mustofa Helmi
Published: November 2020

Abstract. Permatasari DA, Witaningrum AM, Wibisono FJ, Effendi MH. 2020. Detection and prevalence of multidrug-resistant Klebsiella pneumoniae strain isolated from poultry farms in Blitar, Indonesia. Biodiversitas 21: 4642-4647. Antibiotics are commonly used as therapy and disease control in humans and animals. However, the widespread use of antibiotics may also trigger the rise of antibiotic resistance. Therefore, the aim of this study was to study the occurrence of Multidrug-Resistant (MDR) of Klebsiella pneumoniae from poultry farms in Blitar. Chicken cloacal swabs were collected from 63 poultry farms (32 broilers and 31 layers) were taken randomly, using total samples 160 broilers and 155 layers. The collected swab samples were inoculated on MacConkey agar medium for isolation and identification. Single colonies were isolated after primary positive cultures and identified by using the IMViC test and TSIA. 28 (8.88%) out of samples were positive for Klebsiella pneumoniae. The antimicrobial confirmation test showed that 53.57% of the K. pneumoniae isolates were Multi-Drug Resistance (MDR) bacteria. The percentage of MDR bacteria against different antibiotics included ampicillin 75%, Erythromycin 42.86%, Tetracycline 35.72%, Sulfamethoxazole 32.14% and Streptomycin 21.4%. This study confirms that the percentage of resistant isolates of Klebsiella pneumoniae from 5 antimicrobial agents of broiler chicken is higher than layer chicken. The presence of multidrug-resistant bacteria is a threat to public health and livestock. The impact of these conditions leaves a limited treatment option as chicken farmers in Indonesia still using antibiotics without veterinarian supervision.
The ecology of Aedes aegypti and Aedes albopictus larvae habitat in coastal areas of South Sulawesi, Indonesia
Authors: RATNASARI ARINI , JABAL ARIF RAHMAN , RAHMA NUR , RAHMI SRI NUR , KARMILA MILA , Wahid Isra
Published: November 2020

Abstract. Ratnasari A, Jabal AR, Rahma N, Rahmi SN, Karmila M, Wahid I, 2020. The ecology of Aedes aegypti and Aedes albopictus larvae habitat in coastal areas of South Sulawesi, Indonesia. Biodiversitas 21: 4648-4654. Aedes aegypti and Aedes albopictus are arbovirus vectors that can adapt to various environmental conditions. This study aimed to analyze the spatial distribution, habitat characteristics, and the correlation between the number of Ae. aegypti and Ae. albopictus larvae with physical factors. The larvae were collected from seven sampling locations along the coast of South Sulawesi at a distance of 100 m, 500 m, 1 km, and 2 km from the coastline. The collected samples and their water habitat were examined in the Entomology Laboratory of the Faculty of Medicine, Hasanuddin University, South Sulawesi, Indonesia. The number of larvae was counted (3182 larvae of both kinds), and the pH and salinity of the water were measured. Data analysis was performed with IBM SPSS version 24 and spatial distribution of larvae was mapped with ArcGIS version 10.5. We found that the highest number of Ae. aegypti was at a distance of 100 m from the coastline (73.34%), while Ae. albopictus was at 500 m (34.14%). Most of the mosquitos laid their eggs on discarded boats as observed in the Kurri Caddi coast (31.2%). The observed ranges of pH, salinity, and temperature of larvae habitat were 5.5-6.4, 5-2 ppt, and 28-31°C, respectively. The total number of larvae had a significant correlation p<0.05 with the physical factors, i.e., distance from the coastline, water pH, temperature, and kind of water storages (containers). This study showed that physical factors in the coastal area significantly influenced the habitat characteristics and the abundance of Ae. aegypti and Ae. albopictus larvae.
Changes in microbial populations during co-composting of dewatered sewage sludge with pruning wastes in windrow piles
Authors: Nafez Amirhossein , Nikaeen Mahnaz , Hassanzadeh Akbar , Kadkhodaei Safoora
Published: November 2020

Abstract. Nafez AH, Nikaeen M, Hassanzadeh A, Kadkhodaei S. 2020. Changes in microbial populations during co-composting of dewatered sewage sludge with pruning wastes in windrow piles. Biodiversitas 21: 4648-4655. The aim of this paper was to study the composting of sewage sludge (SS) and pruning waste as bulking agent with regard to abiotic factors and succession of functional microbial groups. To prepare the composting piles bulking agent and SS in volumetric ratios of 1:1 (TW1), 2:1(TW2), and 3:1(TW3) were used, and a pile of raw sludge was used as control. Samples for investigation were obtained from the composting piles at time intervals of 5-7 d. The temperature of the control pile and TW1 was just 37°C and did not reach the thermophilic phase. The number of thermophilic bacteria increased to 14 log CFU gDW-1 in the thermophilic phase. The salmonella was lost on the 47th and 19th days of the composting process, for TW2 and TW3, respectively. There was a significant correlation between mesophilic fungi with mesophilic bacteria (r=0.942, P<0.001). The majority of the microbial populations inspected revealed their maximum numbers towards the end of the thermophilic phase, with decreasing tendencies afterward. The number of evaluated microorganisms was controlled by differences in physicochemical parameters at different composting phases. The results revealed that the scheduling of the thermophilic period, as well as the microbial community and environmental situations, affects the microbial structure andTW2 had the best quality compared to other piles in achieving the best thermophilic phase and elimination of pathogenic microorganisms.
The use of effector gene based-markers to facilitate identification of Fusarium sp. infected shallot in Java, Indonesia
Authors: Herlina Lina , ISTIAJI BONJOK
Published: November 2020

Abstract. Herlina L, Istiaji B. 2020. The use of effector gene based-markers to facilitate identification of Fusarium sp. infected shallot in Java, Indonesia. Biodiversitas 21: 4677-4685. One of the most important diseases and become challenge in breeding resistant variety of shallots in Indonesia is Fusarium disease, caused by Fusarium oxysporum f.sp. cepae (FOC). To discriminate the F. oxysporum into forma speciales is uneasy, often managed through laborious and time-intensive disease assays., therefore molecular approaches become the most relevant choice. FOCs from Indonesia were examined by molecular criteria based on putative and effector genes-based markers, i.e.: SIX-genes, C5-gene, and CRX1-2-genes. The alignment analysis discovered some regions which sequences highly contained conserved bases, while amplification result bands vary in size, between 400 - 500 bp. The combined of 7 SIX-genes primers and 3 effector primers (C5 and CRX1-2) used in the clustering analysis of 15 FOC isolates in this study showed that they succeeded in separating 15 FOC isolates into 4 groups through NTSys. Clustering analysis showed that those markers succeeded in grouping 15 FOC isolates into different clades (by coefficient of similarity: 0.69). Phylogenetic analysis based on CRX genes sequence as putative effector genes confirmed that  CRX1 and CRX2 genes were able to classify the FOC into their forma speciales. Those effectors genes are potential to serve as marker-templates to facilitate identification of FOC which infested shallots in Indonesia.