bluuebunny/crossref_metadata_2025 huggingface dataset:
Lipid Emulsion Inhibits the Late Apoptosis/Cardiotoxicity Induced by Doxorubicin in Rat Cardiomyoblasts
Authors: Subbarao Raghavendra Baregundi , Ok Seong-Ho , Lee Soo Hee , Kang Dawon , Kim Eun-Jin , Kim Ji-Yoon , Sohn Ju-Tae
Published: September 2018

This study aimed to examine the effect of lipid emulsion on the cardiotoxicity induced by doxorubicin in H9c2 rat cardiomyoblasts and elucidates the associated cellular mechanism. The effects of lipid emulsion on cell viability, Bax, cleaved caspase-8, cleaved capase-3, Bcl-XL, apoptosis, reactive oxygen species (ROS), malondialdehyde, superoxide dismutase (SOD), catalase and mitochondrial membrane potential induced by doxorubicin were examined. Treatment with doxorubicin decreased cell viability, whereas pretreatment with lipid emulsion reduced the effect of doxorubicin by increasing cell viability. Lipid emulsion also suppressed the increased expression of cleaved caspase-3, cleaved caspase-8, and Bax induced by doxorubicin. Moreover, pretreatment with lipid emulsion decreased the increased Bax/Bcl-XL ratio induced by doxorubicin. Doxorubicin-induced late apoptosis was reduced by treatment with lipid emulsion. In addition, pretreatment with lipid emulsion prior to doxorubicin enhanced glycogen synthase kinase-3β phosphorylation. The increased malondialdehyde and ROS levels by doxorubicin were reduced by lipid emulsion pretreatment. Furthermore, lipid emulsion attenuated the reduced SOD and catalase activity and the decreased mitochondrial membrane potential induced by doxorubicin. Taken together, these results suggest that lipid emulsion attenuates doxorubicin-induced late apoptosis, which appears to be associated with the inhibition of oxidative stress induced by doxorubicin.
Developmental Support for Sick Children through Play in Japan’s ECEC System: A Comparison with Hospital Play Specialists
Authors: Kihara Narumi , Yamamoto Tomoko
Published: September 2018

Early childhood education and care (ECEC) workers and hospital play specialists (HPSs) share a role in supporting the development of sick children through play while respecting their autonomy. On the other hand, in supporting children’s play, managing their anxiety, and making environmental arrangements for them, ECEC workers and HPSs play different roles. When supporting the development of sick children, the former should respect their autonomy and make the most of the characteristics of ECEC as a measure to comprehensively support their participation in play, including it in their daily lives. ECEC workers are also expected to contribute to the further development of comprehensive support by promoting collaboration and cooperation with various professionals, including HPSs.
Perspectives of the Friction Mechanism of Hydrogenated Diamond-Like Carbon Film in Air by Varying Sliding Velocity
Authors: Liu Yunhai , Zhang Bin , Chen Lei , Cao Zhongyue , Shi Pengfei , Liu Jinwei , Zhang Junyan , Qian Linmao
Published: September 2018

The purpose of the present work is to probe the friction mechanism of hydrogenated diamond-like carbon (H-DLC) film in air by varying sliding velocity (25–1000 mm/s). Friction tests of Al2O3 ball against H-DLC film were conducted with a rotational ball-on-disk tribometer. As the sliding velocity increases, both the friction coefficient and the surface wear of H-DLC film decrease, reach the minimum values, and then increase in the high sliding velocity region. Based on the observed results, three main friction mechanisms of H-DLC film—namely graphitization mechanism, transfer layer mechanism, and passivation mechanism—are discussed. Raman analysis indicates that the graphitization of worn surface on the H-DLC film has a negligible contribution to the variation of the friction coefficient and the surface wear. The origin of the sliding velocity dependence is due to the synergistic interaction between the graphitized transfer layer formation and the surface passivation. The present study will not only enrich the understanding of friction mechanism of H-DLC films in air, but will also help to promote their practical engineering applications.
The Crystal Structure of Defect KBB’O6 Pyrochlores (B,B’: Nb,W,Sb,Te) Revisited from Neutron Diffraction Data
Authors: Mayer Sergio F. , Falcón Horacio , Fernández-Díaz María Teresa , Alonso José Antonio
Published: September 2018

Three defect pyrochlores KNbWO6·xH2O, KNbTeO6 and KSbWO6 were synthesized by solid state reaction at 750 °C, from stoichiometric mixtures of K2C2O4, Sb2O3, Nb2O5, WO3 and 20% excess TeO2. A neutron powder diffraction (NPD) data analysis allowed unveiling some structural features. They are all defined in the cubic F d 3 ¯ m space group symmetry, with α = 10.5068(1) Å, 10.2466(1) Å and 10.2377(1) Å, respectively. Difference Fourier synthesis for KNbWO6·xH2O clearly showed the presence of crystallization water, with extra O’ oxygen and H+ atoms that were located from NPD data. These O’ oxygen atoms are placed at 32e Wyckoff sites, conforming a K2O’ sublattice interpenetrated with the covalent framework constituted by (Nb,W)O6 octahedra. The H+ ions coordinate the O’ atoms at partially occupied 96g Wyckoff sites while K+ ions shift also along 32e sites, but closer to the 16c special site (0,0,0). By contrast, extra H2O molecules are absent in the other two pyrochlores: in KNbTeO6 and KSbWO6 K+ ions are shifted along 32e (x,x,x) sites further away from the origin than for the previous material, and the higher covalency of the octahedral network determines more compact structures, with shorter B–O distances and narrower B–O–B angles in the proposed AB2O6 defect pyrochlore structure.
Propylene-Selective Thin Zeolitic Imidazolate Framework Membranes on Ceramic Tubes by Microwave Seeding and Solvothermal Secondary Growth
Authors: Sun Jingze , Yu Chen , Jeong Hae-Kwon
Published: September 2018

Zeolitic imidazolate framework (ZIF-8) membranes have attracted tremendous interest for their high-resolution kinetic separation of propylene/propane mixtures. Current polycrystalline ZIF-8 membranes are supported mostly on planar ceramic substrates (e.g., alumina disks) because of their high thermal, chemical, and mechanical stabilities and facile manufacturing in the labs. Planar supports are, however, not scalable for practical separation applications owing to their low packing density (typically 30–500 m2/m3). On the other hand, ceramic tubes provide order-of-magnitude higher packing densities than planar supports (i.e., much higher membrane areas per module). Here, we report polycrystalline ZIF-8 membranes with thicknesses of ~1.2 μm grown on the bore side of commercially-available ceramic tubes using the microwave seeding and secondary growth technique. The tubular ZIF-8 membranes showed excellent propylene/propane separation factors of ~80, exceeding all currently-reported ZIF-8 membranes on ceramic tubes. It was found that the secondary growth time was critical to enhance the propylene/propane separation factor of the membranes. Membranes were also grown on the shell side of tubular supports, showing the versatility of our technique.
Analytic Study of Complex Fractional Tsallis’ Entropy with Applications in CNNs
Authors: Ibrahim Rabha W. , Darus Maslina
Published: September 2018

In this paper, we study Tsallis’ fractional entropy (TFE) in a complex domain by applying the definition of the complex probability functions. We study the upper and lower bounds of TFE based on some special functions. Moreover, applications in complex neural networks (CNNs) are illustrated to recognize the accuracy of CNNs.
Design of a T-Shaped Antenna Based on Characteristic Mode Manipulation for Metal-Framed Handset Application
Authors: Han Chong-Zhi , Huang Guan-Long , Yuan Tao , Wang Ju-Zhen
Published: September 2018

Recently, metal-framed handset antennas have aroused much attention due to their fantastic appearance and superior robustness. In this paper, a T-shaped antenna suitable for integration with a USB connector is proposed for metal-framed handset application by utilizing the theory of characteristic modes (TCM). Five eigenmodes, one for the lower band and four for the higher band, were merged by a T-shaped metal frame, a feed point, and two shorting pins, as well as three radio frequency (RF) switches so as to broaden the antenna’s operating bandwidth. A prototype was fabricated in order to verify the design concept. Experimental results showed that the proposed antenna was able to cover bandwidths of 0.824–0.960 GHz (GSM band) and 1.710–2.690 GHz (DCS, PCS, UMTS, and LTE bands) with an acceptable radiation efficiency of up to 50% and satisfactory radiation patterns.
Effect of Pristine Palygorskite Powders on Explosion Characteristics of Methane-Air Premixed Gas
Authors: Zhang Yimin , Wang Yan , Zheng Ligang , Yang Tao , Gao Jianliang , Li Zhenhua
Published: September 2018

In this study, pristine palygorskite powders were used as the inhibition materials to suppress the explosion of methane-air premixed gas for the first time. The composition, porosity and pyrolysis characteristics of the powders were tested by X-ray diffraction (XRD), energy dispersive spectrometry (EDS), N2 adsorption-desorption and Thermogravimetry-differential scanning calorimetry (TG-DSC) techniques. The effects of pristine palygorskite powders concentration on the explosion pressure and the average velocity of flame propagation of the 9.5% methane-air premixed gas were tested by a 20 L spherical explosion system and a 5 L pipeline explosion system. The results indicated the pristine palygorskite powders possess a considerable suppression property on methane explosion. When the mass concentration of pristine palygorskite powders was 0.20 g·L−1, the max-pressure of methane explosion was decreased by 23.9%. The methane explosion flame propagation velocity was inhibited obviously. Owing to the excellent inhibitory performance and the advantage of low-cost and environmental harmlessness, pristine palygorskite powders are potential new materials for the application on gas explosion suppression.
Numerical Study on Heat Transfer Characteristics of the 36V Electronic Control Unit System for an Electric Bicycle
Authors: Ekanayake Gihan , Patil Mahesh Suresh , Seo Jae-Hyeong , Lee Moo-Yeon
Published: September 2018

The objective of this study was to numerically investigate the heat transfer characteristics of a 36V electronic control unit (ECU) system of an electric bicycle and to validate the experimental data. The temperatures of the ECU heatsink, seven metal-oxide-silicon field effect transistors (MOSFETs) and two capacitors of the 36V ECU system were numerically derived under variable operating conditions including power dissipation, thermal grease, ambient temperature and heatsink material, to analyze the heat transfer characteristics. When the thermal conductivity of the thermal grease increased from 0.01 W/m °C to 3.0 W/m° C, the temperatures of the seven MOSFETs and the two capacitors decreased by 51.245% and 3.58%, respectively. When the total power dissipation increased from 2.57 MW/m3 to 4.26 MW/m3, the temperatures of the ECU heatsink, seven MOSFETs and the two capacitors increased by 20.95%, 30.31% and 21.54%, respectively. Furthermore, increasing the ambient temperatures from 30 °C to 40 °C resulted in an increase in the temperatures of the ECU heatsink, MOSFET and capacitor by 24.75%, 9.93% and 22.04% respectively.. These numerically derived temperatures for the MOSFET and the ECU heatsink were validated with the experimental results within a range of 7.2% and 1.7%, respectively. This confirmed that the applied numerical model was valid.
Energy and Exergy Analysis of a Cruise Ship
Authors: Baldi Francesco , Ahlgren Fredrik , Nguyen Tuong-Van , Thern Marcus , Andersson Karin
Published: September 2018

In recent years, the International Maritime Organization agreed on aiming to reduce shipping’s greenhouse gas emissions by 50% with respect to 2009 levels. Meanwhile, cruise ship tourism is growing at a fast pace, making the challenge of achieving this goal even harder. The complexity of the energy system of these ships makes them of particular interest from an energy systems perspective. To illustrate this, we analyzed the energy and exergy flow rates of a cruise ship sailing in the Baltic Sea based on measurements from one year of the ship’s operations. The energy analysis allows identifying propulsion as the main energy user (46% of the total) followed by heat (27%) and electric power (27%) generation; the exergy analysis allowed instead identifying the main inefficiencies of the system: while exergy is primarily destroyed in all processes involving combustion (76% of the total), the other main causes of exergy destruction are the turbochargers, the heat recovery steam generators, the steam heaters, the preheater in the accommodation heating systems, the sea water coolers, and the electric generators; the main exergy losses take place in the exhaust gas of the engines not equipped with heat recovery devices. The application of clustering of the ship’s operations based on the concept of typical operational days suggests that the use of five typical days provides a good approximation of the yearly ship’s operations and can hence be used for the design and optimization of the energy systems of the ship.
Modelling Offshore Wave farms for Coastal Process Impact Assessment: Waves, Beach Morphology, and Water Users
Authors: Stokes Christopher , Conley Daniel C.
Published: September 2018

The emerging global wave energy industry has the potential to contribute to the world’s energy needs, but careful consideration of potential impacts to coastal processes in the form of an impact assessment is required for each new wave energy site. Methods for conducting a coastal processes impact assessment for wave energy arrays vary considerably in the scientific literature, particularly with respect to characterising the energy absorption of a wave energy converter (WEC) array in a wave model. In this paper, modelling methods used in the scientific literature to study wave farm impacts on coastal processes are reviewed, with the aim of determining modelling guidance for impact assessments. Effects on wave climate, beach morphology, and the surfing resource for coastal water users are considered. A novel parameterisation for the WEC array transmission coefficient is presented that, for the first time, uses the permitted power rating of the wave farm, which is usually well defined at the impact assessment stage, to estimate the maximum likely absorption of a permitted WEC array. A coastal processes impact assessment case study from a wave farm in south-west Ireland is used to illustrate the application of the reviewed methods, and demonstrates that using the new ‘rated power transmission coefficient’ rather than a WEC-derived transmission coefficient or complete energy absorption scenario can make the difference between significant and non-significant levels of coastal impacts being predicted.
Observations of Partial Discharge Echo in Dielectric Void by Applying a Voltage Chopped Sequence
Authors: Florkowski Marek
Published: September 2018

This paper describes a novel observation on partial discharges (PDs), which occur despite the absence of an applied voltage, within a chopped sequence. It was found that additional discharge pulses occur when a specimen is subjected to chopped sequences rather than a continuous sinusoid. The discharge pulses, called Partial Discharge Echoes (PDE), appeared in time intervals (where no voltage was being applied), immediately after the specimen had been exposed to a base waveform (e.g., sinusoidal) above a partial discharge inception level. The chopped timing is composed of a multiple series of packets consisting of base waveforms, with each packet being separated in time by a defined delay period. The presented experiments were performed on specimens with an embedded gaseous void, in thermosetting insulation and glass as a non-polar material, for comparison. Acquisition of the echo signal was performed in the phase-resolved mode, modifying the synchronization path and the settings. In contrast to continuous sinusoidal PD measurements, the chopped approach might provide a deeper insight into key PD phenomena, such as inception, propagation, time lag, post-discharge time decay, and effective surface area. Special focus was paid to the transition point between sinusoidal phase and the echo interval. The various scenarios of an echo mechanism, depending on the coincidence of remnant polarization field and the field accumulated on void walls, are discussed.
Combined Pitch and Trailing Edge Flap Control for Load Mitigation of Wind Turbines
Authors: He Keshan , Qi Liangwen , Zheng Liming , Chen Yan
Published: September 2018

Using active control methods for load mitigation in wind turbines could greatly reduce the cost of per kilowatt hour of wind power. In this work, the combined pitch and trailing edge flap control (CPFC) for load mitigation of wind turbines is investigated. The CPFC includes an individual pitch control (IPC) loop and a trailing edge flap control (TEFC) loop, which are combined by a load frequency division control algorithm. The IPC loop is mainly used to mitigate the low frequency loads, and the TEFC loop is mainly used to mitigate the high frequency loads. The CPFC adopts both an azimuth angle feed-forward and a loads feedback control strategy. The azimuth angle feed-forward control strategy should mitigate the asymmetrical loads caused by observable disturbances. and the loads feedback control strategy should decrease asymmetrical loads by closed loop control. A simulation is carried out on the joint platform of FAST and MATLAB. The simulation results show that the damage equivalent load (DEL) of blade root out-of-plane bending moment is reduced by 53.7% while using CPFC, compared to collective pitch control (CPC); and the standard deviation of blade tip out-of-plane deflection is reduced by 50.2% while using CPFC, compared to CPC. The results demonstrate that the CPFC can mitigate the fatigue loads of wind turbines as anticipated.
Decision Support for Negotiations among Microgrids Using a Multiagent Architecture
Authors: Pinto Tiago , Fotouhi Ghazvini Mohammad Ali , Soares Joao , Faia Ricardo , Corchado Juan Manuel , Castro Rui , Vale Zita
Published: September 2018

This paper presents a decision support model for negotiation portfolio optimization considering the participation of players in local markets (at the microgrid level) and in external markets, namely in regional markets, wholesale negotiations and negotiations of bilateral agreements. A local internal market model for microgrids is defined, and the connection between interconnected microgrids is based on nodal pricing to enable negotiations between nearby microgrids. The market environment considering the local market setting and the interaction between integrated microgrids is modeled using a multi-agent approach. Several multi-agent systems are used to model the electricity market environment, the interaction between small players at a microgrid scale, and to accommodate the decision support features. The integration of the proposed models in this multi-agent society and interaction between these distinct specific multi-agent systems enables modeling the system as a whole and thus testing and validating the impact of the method in the outcomes of the involved players. Results show that considering the several negotiation opportunities as complementary and making use of the most appropriate markets depending on the expected prices at each moment allows players to achieve more profitable results.
Simulation of the Oxygen Reduction Reaction (ORR) Inside the Cathode Catalyst Layer (CCL) of Proton Exchange Membrane Fuel Cells Using the Kinetic Monte Carlo Method
Authors: Bai Baosheng , Chen Yi-Tung
Published: September 2018

In this paper, a numerical model of the kinetic Monte Carlo (KMC) method has been developed to study the oxygen reduction reaction (ORR) that occurs inside the cathode catalyst layer (CCL). Firstly, a 3-D model of the CCL that consists of Pt and carbon spheres is built using the sphere packing method; secondly, an efficient procedure of the proton-oxygen reaction process is developed and simulated. In the proton-oxygen reaction process, all of the continuous movements of protons and oxygen are considered. The maximum reaction distance is determined to be 8 Å. The input pressures of protons and oxygen are represented by the number of spheres of the species. The value of the current density is calculated based on the amount of reaction during the interval time. Indications are that the results of the present model match reasonably well with the published results. A new way to apply the KMC method in the proton exchange membrane fuel cell (PEMFC) research field is developed in this paper.
Estimation of Forest Above-Ground Biomass by Geographically Weighted Regression and Machine Learning with Sentinel Imagery
Authors: Chen Lin , Ren Chunying , Zhang Bai , Wang Zongming , Xi Yanbiao
Published: September 2018

Accurate forest above-ground biomass (AGB) is crucial for sustaining forest management and mitigating climate change to support REDD+ (reducing emissions from deforestation and forest degradation, plus the sustainable management of forests, and the conservation and enhancement of forest carbon stocks) processes. Recently launched Sentinel imagery offers a new opportunity for forest AGB mapping and monitoring. In this study, texture characteristics and backscatter coefficients of Sentinel-1, in addition to multispectral bands, vegetation indices, and biophysical variables of Sentinal-2, based on 56 measured AGB samples in the center of the Changbai Mountains, China, were used to develop biomass prediction models through geographically weighted regression (GWR) and machine learning (ML) algorithms, such as the artificial neural network (ANN), support vector machine for regression (SVR), and random forest (RF). The results showed that texture characteristics and vegetation biophysical variables were the most important predictors. SVR was the best method for predicting and mapping the patterns of AGB in the study site with limited samples, whose mean error, mean absolute error, root mean square error, and correlation coefficient were 4 × 10−3, 0.07, 0.08 Mg·ha−1, and 1, respectively. Predicted values of AGB from four models ranged from 11.80 to 324.12 Mg·ha−1, and those for broadleaved deciduous forests were the most accurate, while those for AGB above 160 Mg·ha−1 were the least accurate. The study demonstrated encouraging results in forest AGB mapping of the normal vegetated area using the freely accessible and high-resolution Sentinel imagery, based on ML techniques.
Influential Actors’ Perceptions of Facilitators and Instruments for Solving Future Forest Land-Use Disputes in Europe
Authors: Aurenhammer Peter , Ščap Špela , Krajnc Nike , Olivar Jorge , Sabin Pablo , Nobre Sílvia , Romagnoli Francesco
Published: September 2018

Despite strong expectations regarding the role that forestry, with its multitude of potential benefits, could and should play in the ‘bio-economy’, little research has been done on the actual perceptions of influential actors on how to best address future forest land-use disputes. We want to shed light on whether and in which contexts expectations regarding the bio-economy, e.g., the strong role of markets, are likely. The paper analyses influential actors’ core values and beliefs about the primary facilitators and the most appropriate instruments for resolving disputes over future forest land use. We used Social Network Analysis-based sampling and a quantitative semi-structured questionnaire, which included a preference analysis with twelve items covering broad issues and disputes related to future forest land use, to identify actors’ beliefs about and preferences for facilitators and policy instruments within key issues for future land use. The respondents were asked to identify one of five ‘primary facilitators’ (state, market, society, individual citizens/owners, leave it to nature) and distribute six points to a maximum of three preferred instruments (eight items, covering a broad set of instruments, from dictates or bans to awareness raising). The results are based on the perceptions of the influential or most important actors from various innovative government and private forest initiatives in Bavaria (Germany), Slovenia, Castilla y León (Spain), Nordeste (Portugal), and Latvia (481 actor responses, 109 initiatives). The initiatives included participatory mountain forest initiatives, forest intervention zones, afforestation projects, forest owner associations, and model forest and labelling initiatives. The results provide insight into the similarities and differences between European countries and actor groups regarding the preferred facilitators and instruments for solving future forest problems. In light of disagreement in the literature on the role of the state or markets in future forest land use and the bio-economy, our results show that the market and its instruments are considered to play a dominant role in wood mobilisation. With respect to all other issues (socio-ecological, societal, other), the state or other institutions and their instruments gain priority. The state is considered to play a stronger role in developing new markets, e.g., for energy transition or new uses of wood, contrary to liberal market expectations. Ecological and social problems are considered to be outside of the market domain. Here, the state is called in, e.g., to steer recreational issues, the provision of ecosystem services, or the improvement of the protective function. The clearest preference across all regions is for the state to secure the provision of ecosystem services, in contrast to calls for future markets to regulate this field.
National Identities: Temporality and Narration
Authors: Humlebæk Carsten
Published: September 2018

National identities are social phenomena with concrete—both political and social—effects in society, but a fundamental part of their constitution takes place through narratives about the collective. The existence of collective identities thus depends on drawing boundaries between the collective ‘we’ and the ‘others’, as well as on disseminating coherent ideas about the fundamental identity of the we-group. These narratives thus constitute a privileged object for investigating how collective identities are constructed and legitimised in a discourse that places the collective in time, that is, with a coherent and logical narrative about the past and a trustworthy projection into the future. This article defends, first, the concept of the ‘master narrative’ as a useful analytical category for investigating how national history is constructed, and, second, the concepts of ‘sites of memory’ and ‘Vergangenheitsbewältigung’ as means of accessing this narrative. These concepts represent instances of creation and rewriting, respectively, of the narrative and are thus useful tools for analysing how a sense of connectedness with the community through time is created: that is, how a sense of continuity with certain distant epochs is conveyed, and how, on the other hand, a sense of discontinuity with other periods is favoured.
Comprehensive Transcriptome Profiling and Identification of Potential Genes Responsible for Salt Tolerance in Tall Fescue Leaves under Salinity Stress
Authors: Amombo Erick , Li Xiaoning , Wang Guangyang , An Shao , Wang Wei , Fu Jinmin
Published: September 2018

Soil salinity is a serious threat to plant growth and crop productivity. Tall fescue utilization in saline areas is limited by its inferior salt tolerance. Thus, a transcriptome study is a prerequisite for future research aimed at providing deeper insights into the molecular mechanisms of tall fescue salt tolerance as well as molecular breeding. Recent advances in sequencing technology offer a platform to achieve this. Here, Illumina RNA sequencing of tall fescue leaves generated a total of 144,339 raw reads. After de novo assembly, unigenes with a total length of 129,749,938 base pairs were obtained. For functional annotations, the unigenes were aligned to various databases. Further structural analyses revealed 79,352 coding DNA sequences and 13,003 microsatellites distributed across 11,277 unigenes as well as single nucleotide polymorphisms. In total, 1862 unigenes were predicted to encode for 2120 transcription factors among which most were key salt-responsive. We determined differential gene expression and distribution per sample and most genes related to salt tolerance and photosynthesis were upregulated in 48 h vs. 24 h salt treatment. Protein interaction analysis revealed a high interaction of chaperonins and Rubisco proteins in 48 h vs. 24 h salt treatment. The gene expressions were finally validated using quantitative polymerase chain reaction (qPCR), which was coherent with sequencing results.
Novel Methylselenoesters Induce Programed Cell Death via Entosis in Pancreatic Cancer Cells
Authors: Khalkar Prajakta , Díaz-Argelich Nuria , Antonio Palop Juan , Sanmartín Carmen , Fernandes Aristi P.
Published: September 2018

Redox active selenium (Se) compounds have gained substantial attention in the last decade as potential cancer therapeutic agents. Several Se compounds have shown high selectivity and sensitivity against malignant cells. The cytotoxic effects are exerted by their biologically active metabolites, with methylselenol (CH3SeH) being one of the key executors. In search of novel CH3SeH precursors, we previously synthesized a series of methylselenoesters that were active (GI50 < 10 µM at 72 h) against a panel of cancer cell lines. Herein, we refined the mechanism of action of the two lead compounds with the additional synthesis of new analogs (ethyl, pentyl, and benzyl derivatives). A novel mechanism for the programmed cell death mechanism for Se-compounds was identified. Both methylseleninic acid and the novel CH3SeH precursors induced entosis by cell detachment through downregulation of cell division control protein 42 homolog (CDC42) and its downstream effector β1-integrin (CD29). To our knowledge, this is the first time that Se compounds have been reported to induce this type of cell death and is of importance in the characterization of the anticancerogenic properties of these compounds.
Neglected Functions of TFCP2/TFCP2L1/UBP1 Transcription Factors May Offer Valuable Insights into Their Mechanisms of Action
Authors: Taracha Agnieszka , Kotarba Grzegorz , Wilanowski Tomasz
Published: September 2018

In recent years, the TFCP2 (transcription factor cellular promoter 2)/TFCP2L1 (TFCP2-like 1)/UBP1 (upstream binding protein 1) subfamily of transcription factors has been attracting increasing attention in the scientific community. These factors are very important in cancer, Alzheimer’s disease, and other human conditions, and they can be attractive targets for drug development. However, the interpretation of experimental results is complicated, as in principle, any of these factors could substitute for the lack of another. Thus, studying their hitherto little known functions should enhance our understanding of mechanisms of their functioning, and analogous mechanisms might govern their functioning in medically relevant contexts. For example, there are numerous parallels between placental development and cancer growth; therefore, investigating the roles of TFCP2, TFCP2L1, and UBP1 in the placenta may help us better understand their functioning in cancer, as is evidenced by the studies of various other proteins and pathways. Our review article aims to call the attention of the scientific community to these neglected functions, and encourage further research in this field. Here, we present a systematic review of current knowledge of the TFCP2/TFCP2L1/UBP1 subfamily in reproduction, embryonic development, renal function, blood-pressure regulation, brain function, and other processes, where their involvement has not been studied much until now.
The Effects of High Fat Diet-Induced Stress on Olfactory Sensitivity, Behaviors, and Transcriptional Profiling in Drosophila melanogaster
Authors: Jung Jewon , Kim Dong-In , Han Gi-Youn , Kwon Hyung Wook
Published: September 2018

High-fat diet (HFD) often causes obesity and it has detrimental effects on the sensory system. In particular, sensory-mediated responses are crucial for maintaining energy balance, as they are involved in a metabolic regulation; however, there is still no clear explanation about the relationship between HFD-induced stress and sensory system. To gain insight on how HFD-induced stress affects olfactory sensitivity and behavioral responses, we have used a Drosophila melanogaster model for olfactory and nutrient-related signaling and accessed physiological, behavioral, and transcriptional changes. We demonstrated that lifespan and climbing ability in HFD-treated flies decreased and that olfactory sensitivity and behavioral responses to odorants were changed. Olfactory sensitivity to eight of ten odorants after 14 days on HFD treatment were reduced, while behavioral attraction was increased to benzaldehyde in flies that were treated with HFD. This behavioral and physiological modification in HFD-treated flies for 14 days was accompanied by a significant decrease in DmOrco gene expression in a peripheral olfactory organ, suggesting that is could be involved in the action of metabolic and sensory signal. Gene expression profiles of antennae showed significant differences on the olfactory receptors, odorant-binding proteins, and insulin signaling. Our results suggested that olfactory sensitivity and behavioral responses to HFD-induced stress are mediated through olfactory and nutrient-related signaling pathways.
Role of the Genetic Background in Resistance to Plant Viruses
Authors: Gallois Jean-Luc , Moury Benoît , German-Retana Sylvie
Published: September 2018

In view of major economic problems caused by viruses, the development of genetically resistant crops is critical for breeders but remains limited by the evolution of resistance-breaking virus mutants. During the plant breeding process, the introgression of traits from Crop Wild Relatives results in a dramatic change of the genetic background that can alter the resistance efficiency or durability. Here, we conducted a meta-analysis on 19 Quantitative Trait Locus (QTL) studies of resistance to viruses in plants. Frequent epistatic effects between resistance genes indicate that a large part of the resistance phenotype, conferred by a given QTL, depends on the genetic background. We next reviewed the different resistance mechanisms in plants to survey at which stage the genetic background could impact resistance or durability. We propose that the genetic background may impair effector-triggered dominant resistances at several stages by tinkering the NB-LRR (Nucleotide Binding-Leucine-Rich Repeats) response pathway. In contrast, effects on recessive resistances by loss-of-susceptibility—such as eIF4E-based resistances—are more likely to rely on gene redundancy among the multigene family of host susceptibility factors. Finally, we show how the genetic background is likely to shape the evolution of resistance-breaking isolates and propose how to take this into account in order to breed plants with increased resistance durability to viruses.
Identification of Endogenous Control miRNAs for RT-qPCR in T-Cell Acute Lymphoblastic Leukemia
Authors: Drobna Monika , Szarzyńska-Zawadzka Bronisława , Daca-Roszak Patrycja , Kosmalska Maria , Jaksik Roman , Witt Michał , Dawidowska Małgorzata
Published: September 2018

Optimal endogenous controls enable reliable normalization of microRNA (miRNA) expression in reverse-transcription quantitative PCR (RT-qPCR). This is particularly important when miRNAs are considered as candidate diagnostic or prognostic biomarkers. Universal endogenous controls are lacking, thus candidate normalizers must be evaluated individually for each experiment. Here we present a strategy that we applied to the identification of optimal control miRNAs for RT-qPCR profiling of miRNA expression in T-cell acute lymphoblastic leukemia (T-ALL) and in normal cells of T-lineage. First, using NormFinder for an iterative analysis of miRNA stability in our miRNA-seq data, we established the number of control miRNAs to be used in RT-qPCR. Then, we identified optimal control miRNAs by a comprehensive analysis of miRNA stability in miRNA-seq data and in RT-qPCR by analysis of RT-qPCR amplification efficiency and expression across a variety of T-lineage samples and T-ALL cell line culture conditions. We then showed the utility of the combination of three miRNAs as endogenous normalizers (hsa-miR-16-5p, hsa-miR-25-3p, and hsa-let-7a-5p). These miRNAs might serve as first-line candidate endogenous controls for RT-qPCR analysis of miRNAs in different types of T-lineage samples: T-ALL patient samples, T-ALL cell lines, normal immature thymocytes, and mature T-lymphocytes. The strategy we present is universal and can be transferred to other RT-qPCR experiments.
New Insight into the Chloroacetanilide Herbicide Degradation Mechanism through a Nucleophilic Attack of Hydrogen Sulfide
Authors: Mora José R. , Cervantes Cristian , Marquez Edgar
Published: September 2018

The nucleophilic attack of hydrogen sulfide (HS−) on six different chloroacetanilide herbicides was evaluated theoretically using the dispersion-corrected hybrid functional wB97XD and the 6-311++G(2d,2p) Pople basis sets. The six evaluated substrates were propachlor (A), alachlor (B), metolachlor (C), tioacetanilide (D), β-anilide (E), and methylene (F). Three possible mechanisms were considered: (a) bimolecular nucleophilic substitution (SN2) reaction mechanism, (b) oxygen assistance, and (c) nitrogen assistance. Mechanisms based on O- and N-assistance were discarded due to a very high activation barrier in comparison with the corresponding SN2 mechanism, with the exception of compound F. The N-assistance mechanism for compound F had a free activation energy of 23.52 kcal/mol, which was close to the value for the corresponding SN2 mechanism (23.94 kcal/mol), as these two mechanisms could occur in parallel reactions with almost 50% of each one. In compounds A to D, an important electron-withdrawing effect of the C=O and C=S groups was seen, and consequently, the activation free energies in these SN2 reactions were smaller, with a value of approximately 18 kcal/mol. Instead, compounds E and F, which have a CH2 group in the β-position, presented a higher activation free energy (≈22 kcal/mol). Good agreement was found between experimental and theoretical values for all cases, and a reaction force analysis was performed on the intrinsic reaction coordinate profile in order to gain more details about the reaction mechanism. Finally, from the natural bond orbital (NBO) analysis, it was possible to evaluate the electronic reorganization through the reaction pathway where all the transition states were early in nature in the reaction coordinate (δBav < 50%); the transition states corresponding to compounds A to D turned out to be more synchronous than those for compounds E and F.
Hormesis: Path and Progression to Significance
Authors: Calabrese Edward J.
Published: September 2018

This paper tells the story of how hormesis became recognized as a fundamental concept in biology, affecting toxicology, microbiology, medicine, public health, agriculture, and all areas related to enhancing biological performance. This paper assesses how hormesis enhances resilience to normal aging and protects against a broad spectrum of neurodegenerative, cardiovascular, and other diseases, as well as trauma and other threats to health and well-being. This paper also explains the application of hormesis to several neurodegenerative diseases such as Parkinson’s and Huntington’s disease, macrophage polarization and its systematic adaptive protections, and the role of hormesis in enhancing stem cell functioning and medical applications.
Distribution of Canthon rutilans rutilans and Canthon rutilans cyanescens Along Spatio-Temporal and Temperature Gradients
Authors: Hensen Maristela Carpintero , Hernández Malva Isabel Medina , Da Silva Pedro Giovâni , Amore Valentina , Lobo Jorge M.
Published: September 2018

Subspecies is a debated taxonomic rank that, in some cases, could indicate that a speciation process is taking place. Studying the degree of co-occurrence among subspecies along environmental gradients may help to determine its taxonomic status. In this study, we explore the distribution of two subspecies of Canthon rutilans along spatio-temporal and temperature gradients in the Atlantic Forest of southern Brazil in order to reinforce their current subspecies status or to support their consideration as two different species. A yearly survey conducted along an elevational transect (from 250 m to 1630 m) shows that there is no spatio-temporal overlap between the two taxa. We collected 899 individuals of Canthon rutilans cyanescens and 29 individuals of Canthon rutilans rutilans. C. rutilans cyanescens can be found at 250 m (all year except in June), 430 m (August to April), and 840 m (September to April) in elevation, and when the air temperature oscillates from 15.3 °C to 24.0 °C. C. rutilans rutilans can be found at 1360 m (October to February), 1630 m (January) in elevation, and when the air temperature oscillates from 14.4 °C to 18.6 °C. Furthermore, local temperature data taken during the survey indicates that both subspecies also have a limited overlap in their thermal response curves. All these results suggest that these two taxa could be considered as two different species with dissimilar physiological and ecological requirements probably as a consequence of temperature-mediated divergent adaptation. Further molecular data can confirm or reject this supposition in the near future.
Influence of the Composition on the Environmental Impact of Soft Ferrites
Authors: Gómez Patricia , Elduque Daniel , Pina Carmelo , Javierre Carlos
Published: September 2018

The aim of this paper is to analyze the influence of the composition on the environmental impact of the two main types of soft ferrites, allowing scientists and engineers to compare them based not only on cost and properties, but also on an environmental point of view. Iron oxides are the basis of soft ferrites, but these ferrites have a wide range of compositions, using materials such as manganese or nickel, which affect their magnetic properties, but also modify the environmental impact. A Life Cycle Assessment has been carried out for manganese‒zinc (MnZn) and nickel‒zinc (NiZn) soft ferrites, with a Monte Carlo approach to assess multiple compositions. The LCA model was developed with SimaPro 8.4, using the EcoInvent v3.4 life cycle inventory database. Environmental impact values were calculated under the ReCiPe and Carbon Footprint methodologies, obtaining a broad variety of results depending on the composition. The results were also significantly different from the standard EcoInvent ferrite. For the analyzed soft ferrites, the presence of manganese or nickel is a key factor from an environmental perspective, as these materials involve high environmental impacts, and their supply risk has increased during recent years, making them a concern for European manufacturers.
Enhanced Photoelectrochemical Properties of Ti3+ Self-Doped Branched TiO2 Nanorod Arrays with Visible Light Absorption
Authors: Wang Jingyang , Wang Xiantao , Yan Jun , Tan Qi , Liang Guijie , Qu Shaohua , Zhong Zhicheng
Published: September 2018

A novel Ti3+ self-doped branched rutile TiO2 nanorod arrays (NRAs) was successfully grown on an F-doped tin oxide (FTO) transparent conductive glass by a combined hydrothermal and magnetron sputtering method. Surface morphology, structure, optical properties, and photoelectrochemical behavior of the branched TiO2 NRAs are determined. Using TiO2 nanoparticles (NPs) deposited on the top of the nanorods as seeds, TiO2 nanobranches can easily grow on the top of the nanorods. Moreover, the Ti3+ defects in the TiO2 NPs and associated oxygen vacancies, and the nanobranches expend the optical absorption edge of the TiO2 NRAs from 400 nm to 510 nm. Branched TiO2 NRAs exhibit excellent photoelectrochemical properties compared to the pure TiO2 NRAs, as revealed by photoelectrochemical measurements. This enhanced photoelectrochemical properties is induced by the increased surface area and expanded optical absorption range. Due to their favorable characteristics, these novel branched TiO2 NRAs will provide a new path to the fabrication of hierarchical nanostructured materials.
Dynamic Flow Characteristics and Design Principles of Laminar Flow Microbial Fuel Cells
Authors: Cheng Way Lee , Erbay Celal , Sadr Reza , Han Arum
Published: September 2018

Laminar flow microbial fuel cells (MFCs) are used to understand the role of microorganisms, and their interactions with electrodes in microbial bioelectrochemical systems. In this study, we reported the flow characteristics of laminar flow in a typical MFC configuration in a non-dimensional form, which can serve as a guideline in the design of such microfluidic systems. Computational fluid dynamics simulations were performed to examine the effects of channel geometries, surface characteristics, and fluid velocity on the mixing dynamics in microchannels with a rectangular cross-section. The results showed that decreasing the fluid velocity enhances mixing but changing the angle between the inlet channels, only had strong effects when the angle was larger than 135°. Furthermore, different mixing behaviors were observed depending on the angle of the channels, when the microchannel aspect ratio was reduced. Asymmetric growth of microbial biofilm on the anode side skewed the mixing zone and wall roughness due to the bacterial attachment, which accelerated the mixing process and reduced the efficiency of the laminar flow MFC. Finally, the magnitude of mass diffusivity had a substantial effect on mixing behavior. The results shown here provided both design guidelines, as well as better understandings of the MFCs due to microbial growth.
Optimizing Flotation Circuit Recovery by Effective Stage Arrangements: A Case Study
Authors: Radmehr Vahid , Shafaei Sied Ziaedin , Noaparast Mohammad , Abdollahi Hadi
Published: September 2018

Recovery is one of the most important metallurgical parameters in designing and evaluating flotation circuits. The present study used the recovery arrangement for two and three stage circuits to evaluate the effect of stage recovery on the overall circuit recovery and flotation circuit configuration. The results showed that mainly the highest recovery value should be assigned to the rougher stage in order to achieve the maximum overall circuit recovery. Countercurrent rougher-cleaner and rougher-scavenger circuits, in which recycling streams step back one stage at a time, follow a general rule for the assignment of recovery. Finally, a flotation plant containing six flotation banks was examined as a case study. A program for calculating total circuit recovery, for all possible combinations of recovery was developed in MATLAB software. 720 recovery combinations were evaluated. The results showed that optimal recovery allocation in stages could be effective in achieving overall circuit recovery. It was shown that the use of a large number of stages in some of the flotation circuits leads to the loss of equipment and additional costs. The proposed approach can be employed as an effective tool for designing and optimizing various flotation circuits and their operational parameters.