bluuebunny/crossref_metadata_2025 huggingface dataset:
Cyclonic Wave Simulations Based on WAVEWATCH-III Using a Sea Surface Drag Coefficient Derived from CFOSAT SWIM Data
Authors: Shao Weizeng , Jiang Tao , Zhang Yu , Shi Jian , Wang Weili
Published: December 2021

It is well known that numerical models are powerful methods for wave simulation of typhoons, where the sea surface drag coefficient is sensitive to strong winds. With the development of remote sensing techniques, typhoon data (i.e., wind and waves) have been captured by optical and microwave satellites such as the Chinese-French Oceanography SATellite (CFOSAT). In particular, wind and wave spectra data can be simultaneously measured by the Surface Wave Investigation and Monitoring (SWIM) onboard CFOSAT. In this study, existing parameterizations for the drag coefficient are implemented for typhoon wave simulations using the WAVEWATCH-III (WW3) model. In particular, a parameterization of the drag coefficient derived from sea surface roughness is adopted by considering the terms for wave steepness and wave age from the measurements from SWIM products of CFOSAT from 20 typhoons during 2019–2020 at winds up to 30 m/s. The simulated significant wave height (Hs) from the WW3 model was validated against the observations from several moored buoys active during three typhoons, i.e., Typhoon Fung-wong (2014), Chan-hom (2015), and Lekima (2019). The analysis results indicated that the proposed parameterization of the drag coefficient significantly improved the accuracy of typhoon wave estimation (a 0.49 m root mean square error (RMSE) of Hs and a 0.35 scatter index (SI)), greater than the 0.55 RMSE of Hs and >0.4 SI using other existing parameterizations. In this sense, the adopted parameterization for the drag coefficient is recommended for typhoon wave simulations using the WW3 model, especially for sea states with Hs < 7 m. Moreover, the accuracy of simulated waves was not reduced with growing winds and sea states using the proposed parameterization. However, the applicability of the proposed parameterization in hurricanes necessitates further investigation at high winds (>30 m/s).
Heat Transfer in Biological Spherical Tissues during Hyperthermia of Magnetoma
Authors: Ragab Mahmoud , Abouelregal Ahmed E. , AlShaibi Huda F. , Mansouri Rasha A.
Published: December 2021

Hyperthermia therapy is now being used to treat cancer. However, understanding the pattern of temperature increase in biological tissues during hyperthermia treatment is essential. In recent years, many physicians and engineers have studied the use of computational and mathematical models of heat transfer in biological systems. The rapid progress in computing technology has intrigued many researchers. Many medical procedures also use engineering techniques and mathematical modeling to ensure their safety and assess the risks involved. One such model is the modified Pennes bioheat conduction equation. This paper provides an analytical solution to the modified Pennes bioheat conduction equation with a single relaxation time by incorporating in it the (MGT) equation. The suggested model examines heat transport in biological tissues as forming an infinite concentric spherical region during magnetic fluid hyperthermia. To investigate thermal reactions caused by temperature shock, specifically the influence of heat generation through heat treatment on a skin tumor [AEGP9], the Laplace transformation, and numerical inverse transformation methods are used. This model was able to explain the effects of different therapeutic approaches such as cryotherapy sessions, laser therapy, and physical occurrences, transfer, metabolism support, and blood perfusion. Comparison of the numerical results of the suggested model with those in the literature confirmed the validity of the model’s numerical results.
Soil Rehabilitation Promotes Resilient Microbiome with Enriched Keystone Taxa than Agricultural Infestation in Barren Soils on the Loess Plateau
Authors: Liu Dong , Bhople Parag , Keiblinger Katharina Maria , Wang Baorong , An Shaoshan , Yang Nan , Chater Caspar C. C. , Yu Fuqiang
Published: December 2021

Drylands provide crucial ecosystem and economic services across the globe. In barren drylands, keystone taxa drive microbial structure and functioning in soil environments. In the current study, the Chinese Loess plateau’s agricultural (AL) and twenty-year-old rehabilitated lands (RL) provided a unique opportunity to investigate land-use-mediated effects on barren soil keystone bacterial and fungal taxa. Therefore, soils from eighteen sites were collected for metagenomic sequencing of bacteria specific 16S rRNA and fungi specific ITS2 regions, respectively, and to conduct molecular ecological networks and construct microbial OTU-based correlation matrices. In RL soils we found a more complex bacterial network represented by a higher number of nodes and links, with a link percentage of 77%, and a lower number of nodes and links for OTU-based fungal networks compared to the AL soils. A higher number of keystone taxa was observed in the RL (66) than in the AL (49) soils, and microbial network connectivity was positively influenced by soil total nitrogen and microbial biomass carbon contents. Our results indicate that plant restoration and the reduced human interventions in RL soils could guide the development of a better-connected microbial network and ensure sufficient nutrient circulation in barren soils on the Loess plateau.
Initial Evaluation of Uroplakins UPIIIa and UPII in Selected Benign Urological Diseases
Authors: Szymańska Beata , Matuszewski Michał , Dembowski Janusz , Piwowar Agnieszka
Published: December 2021

Background: Uroplakins (UPs) are glycoproteins that play a specific role in the structure and function of the urothelium. Disorders which affect the normal expression of UPs are associated with the pathogenesis of infections and neoplasms of the urinary tract, primary vesicoureteral reflux, hydronephrosis and renal dysfunction. The appearance of uroplakins in the urine and/or plasma may be of potential importance in the detection of urinary tract dysfunction. The aim of the present study was to investigate uroplakin IIIa (UPIIIa) and uroplakin II (UPII) expression in patients with selected urological diseases. Methods: Plasma and urine from patients with benign prostatic hyperplasia (BPH), urethral stricture (US), urinary tract infection (UTI) and urolithiasis were compared to healthy people without urological disorders. UPs concentrations were measured by the immunoenzymatic method. Results: In patients with BPH and UTI, concentrations of UPIIIa in urine and plasma, as well as UPII in urine, were statistically significantly higher than in the control groups. In the US group, only the plasma UPIIIa concentration differed significantly from the control. Conclusion: The conducted research shows that benign urological diseases may affect the state of the urothelium, as manifested by increased concentrations of both UPs in patients’ urine and plasma, especially in BPH and UTI.
Characteristics and Outcomes in Primary Aldosteronism Patients Harboring Glucocorticoid-Remediable Aldosteronism
Authors: Cheng Chung-Yi , Liao Hung-Wei , Peng Kang-Yung , Chen Tso-Hsiao , Lin Yen-Hung , Chueh Jeff S. , Wu Vin-Cent ,   on behalf of the TAIPAI Study Group
Published: December 2021

The clinical characteristics and surgical prognosis of glucocorticoid-remediable aldosteronism (GRA, also known as familial hyperaldosteronism type 1, FH-I) have not been widely studied. Using data from the Taiwan Primary Aldosteronism Investigation (TAIPAI) registry retrospectively, we describe the associated clinical factors for GRA and clinical predictors of surgical outcomes among identified GRA patients. We found 79 GRA-positive (51.2 ± 13.8 years; women 39 (49.4%)) and 114 GRA-negative primary aldosteronism (PA) patients matched with age, gender, and body mass index. Lower plasma aldosterone concentrations (PACs) and aldosterone-renin ratios were found among GRA-positive individuals. Multivariable logistic regression demonstrated that a PAC ≤ 40 ng/dL could predict concealed GRA individuals (OR 0.523, p = 0.037). Low serum potassium (OR 0.285, p = 0.008), but not the presence of GRA, was associated with hypertension-remission. Of note, PRA (OR 11.645, p = 0.045) and hypokalemia (OR 0.133, p = 0.048) were associated with hypertension-remission in GRA patients. Unilateral primary aldosteronism patients harboring concomitant GRA were not associated with inferior hypertension-remission after an adrenalectomy. Low serum potassium and high PRA were positively associated with hypertension-remission in GRA patients.
Dissecting the Transcriptomes of Multiple Metronidazole-Resistant and Sensitive Trichomonas vaginalis Strains Identified Distinct Genes and Pathways Associated with Drug Resistance and Cell Death
Authors: Huang Po-Jung , Huang Ching-Yun , Li Yu-Xuan , Liu Yi-Chung , Chu Lichieh-Julie , Yeh Yuan-Ming , Cheng Wei-Hung , Chen Ruei-Ming , Lee Chi-Ching , Chen Lih-Chyang , Lin Hsin-Chung , Chiu Shu-Fang , Lin Wei-Ning , Lyu Ping-Chiang , Tang Petrus , Huang Kuo-Yang
Published: December 2021

Trichomonas vaginalis is the causative agent of trichomoniasis, the most prevalent non-viral sexually transmitted infection worldwide. Metronidazole (MTZ) is the mainstay of anti-trichomonal chemotherapy; however, drug resistance has become an increasingly worrying issue. Additionally, the molecular events of MTZ-induced cell death in T. vaginalis remain elusive. To gain insight into the differential expression of genes related to MTZ resistance and cell death, we conducted RNA-sequencing of three paired MTZ-resistant (MTZ-R) and MTZ-sensitive (MTZ-S) T. vaginalis strains treated with or without MTZ. Comparative transcriptomes analysis identified that several putative drug-resistant genes were exclusively upregulated in different MTZ-R strains, such as ATP-binding cassette (ABC) transporters and multidrug resistance pumps. Additionally, several shared upregulated genes among all the MTZ-R transcriptomes were not previously identified in T. vaginalis, such as 5′-nucleotidase surE and Na+-driven multidrug efflux pump, which are a potential stress response protein and a multidrug and toxic compound extrusion (MATE)-like protein, respectively. Functional enrichment analysis revealed that purine and pyrimidine metabolisms were suppressed in MTZ-S parasites upon drug treatment, whereas the endoplasmic reticulum-associated degradation (ERAD) pathway, proteasome, and ubiquitin-mediated proteolysis were strikingly activated, highlighting the novel pathways responsible for drug-induced stress. Our work presents the most detailed analysis of the transcriptional changes and the regulatory networks associated with MTZ resistance and MTZ-induced signaling, providing insights into MTZ resistance and cell death mechanisms in trichomonads.
SYNE1 Exonic Variant rs9479297 Contributes to Concurrent Hepatocellular and Transitional Cell Carcinoma Double Primary Cancer
Authors: Chu Yu-De , Kee Kwong-Ming , Lin Wey-Ran , Lai Ming-Wei , Lu Sheng-Nan , Chung Wen-Hung , Pang See-Tong , Yeh Chau-Ting
Published: December 2021

Unexpected high risk of synchronous/metachronous hepatocellular carcinoma (HCC) and transitional cell carcinoma (TCC) co-occurrence has been discovered previously. Here, we searched for genetic variation contributing to the co-occurrence of this double primary cancer (DPC). Using targeted exome sequencing, a panel of variants associated with concurrent DPC was identified. However, only a nonsynonymous variant within the Spectrin Repeat Containing Nuclear Envelope Protein 1 (SYNE1) gene was associated with DPC occurrence (p = 0.002), compared with that in the healthy population. Further independent cohort verification analysis revealed that the SYNE1-rs9479297-TT genotype (versus TC + CC genotypes) was enriched in patients with DPC, compared with that in those with TCC alone (p = 0.039), those with HCC alone (p = 0.006), those with non-HCC/non-TCC (p < 0.001), and healthy population (p < 0.001). SYNE1 mRNA expression reduced in both patients with HCC and TCC, and its lower expression in HCC was associated with shorter recurrence-free (p = 0.0314) and metastasis-free (p = 0.0479) survival. SYNE1-rs9479297 genotypes were correlated with tissue SYNE1 levels and clinical outcomes in HCC patients. Finally, SYNE1 silencing enhanced the cell proliferation and migration of HCC/TCC cells. In conclusion, SYNE1-rs9479297 genotypes were associated with HCC/TCC DPC co-occurrence and correlated with SYNE1 expression, which in turn contributed to HCC/TCC cell proliferation and migration, thereby affecting clinical outcomes.
Validity and Reliability of the Baby and Child Eating Behavior Questionnaire, Toddler Version (BEBQ-Mex and CEBQ-T-Mex) in a Low Sociodemographic Sample Recruited in a Mexican Hospital
Authors: Hunot-Alexander Claudia , González-Toribio Jocelyn , Vásquez-Garibay Edgar Manuel , Larrosa-Haro Alfredo , Casillas-Toral Erika , Curiel-Curiel Carmen Patricia
Published: December 2021

The objective of this study was to validate and measure the internal reliability of the Baby and Child Eating Behavior Questionnaires for Toddlers (BEBQ-Mex and CEBQ-T-Mex), that evaluate appetitive trait (ATs). Mothers recruited from a public hospital in Guadalajara, Mexico, completed the BEBQ-Mex or CEBQ-T-Mex along with information on sociodemographic characteristics. Internal reliability of the BEBQ-Mex was sufficient for Food Responsiveness (FR) (Cronbach α = 0.82), while Enjoyment of Food (EF) and Satiety Responsiveness (SR) showed poor reliability (α = 0.56) and Slowness in Eating (SE) had unacceptable reliability (a = 0.36). All reliability values for the CEBQ-T-Mex were acceptable (>0.70), except for SE (α = 0.64). Confirmatory factor analysis (CFA) revealed an adequate model fit for the BEBQ-Mex, except the SE subscale. CFA for the CEBQ-T-Mex confirmed the six-factor structure. Mothers of a low sociodemographic background were unable to recognize their infants’ ATs; the BEBQ-Mex partly met the criteria for validity and reliability. Mothers from similar sociodemographic characteristics were more able to recognize the ATs of their toddlers than their infants; the CEBQ-T-Mex was found to be a valid and reliable tool. Findings support the need to help mothers’ ability to recognize their infants’ ATs, which have been previously associated with weight and growth.
Multimodality Management of EBV-Associated Nasopharyngeal Carcinoma
Authors: Yu Justin , Pham Tiffany , Wandrey Narine , Daly Mackenzie , Karam Sana
Published: December 2021

Nasopharyngeal carcinoma (NPC) is a rare cancer of the nasopharyngeal mucosa with a specific geographic predisposition. NPC is often associated with Epstein–Barr Virus (EBV) infection and as a result contains many characteristic biomarkers. Treatment of locally-contained NPC is generally achieved through use of radiotherapy (RT), as part of a multimodality treatment regimen. Induction chemotherapy followed by concurrent RT and platinum-based chemotherapy regimen has emerged as the definitive treatment of choice for locoregionally-advanced NPC. Recently, immunotherapy is finding a role in the treatment of recurrent or metastatic NPC. Immune checkpoint blockade therapies targeted against the programmed death-1 (PD-1) receptor have demonstrated efficacy in early phase clinical trials, with ongoing phase III trials in effect. Biomarkers for treatment efficacy remain an ongoing area of investigation, with important prognostic implications on the horizon.
The GRP94 Inhibitor PU-WS13 Decreases M2-like Macrophages in Murine TNBC Tumors: A Pharmaco-Imaging Study with 99mTc-Tilmanocept SPECT
Authors: Bouchard Alexanne , Sikner Hugo , Baverel Valentin , Garnier Anaïs-Rachel , Monterrat Marie , Moreau Mathieu , Limagne Emeric , Garrido Carmen , Kohli Evelyne , Collin Bertrand , Bellaye Pierre-Simon
Published: December 2021

Triple-negative breast cancer (TNBC) is the most aggressive subtype of breast cancers and is not eligible for hormone and anti-HER2 therapies. Identifying therapeutic targets and associated biomarkers in TNBC is a clinical challenge to improve patients’ outcome and management. High infiltration of CD206+ M2-like macrophages in the tumor microenvironment (TME) indicates poor prognosis and survival in TNBC patients. As we previously showed that membrane expression of GRP94, an endoplasmic reticulum chaperone, was associated with the anti-inflammatory profile of human PBMC-derived M2 macrophages, we hypothesized that intra-tumoral CD206+ M2 macrophages expressing GRP94 may represent innovative targets in TNBC for theranostic purposes. We demonstrate in a preclinical model of 4T1 breast tumor-bearing BALB/c mice that (i) CD206-expressing M2-like macrophages in the TME of TNBC can be specifically detected and quantified using in vivo SPECT imaging with 99mTc-Tilmanocept, and (ii) the inhibition of GRP94 with the chemical inhibitor PU-WS13 induces a decrease in CD206-expressing M2-like macrophages in TME. This result correlated with reduced tumor growth and collagen content, as well as an increase in CD8+ cells in the TME. 99mTc-Tilmanocept SPECT imaging might represent an innovative non-invasive strategy to quantify CD206+ tumor-associated macrophages as a biomarker of anti-GRP94 therapy efficacy and TNBC tumor aggressiveness.
Endocannabinoid System and Tumour Microenvironment: New Intertwined Connections for Anticancer Approaches
Authors: Iozzo Marta , Sgrignani Giovanna , Comito Giuseppina , Chiarugi Paola , Giannoni Elisa
Published: December 2021

The tumour microenvironment (TME) is now recognised as a hallmark of cancer, since tumour:stroma crosstalk supports the key steps of tumour growth and progression. The dynamic co-evolution of the tumour and stromal compartments may alter the surrounding microenvironment, including the composition in metabolites and signalling mediators. A growing number of evidence reports the involvement of the endocannabinoid system (ECS) in cancer. ECS is composed by a complex network of ligands, receptors, and enzymes, which act in synergy and contribute to several physiological but also pathological processes. Several in vitro and in vivo evidence show that ECS deregulation in cancer cells affects proliferation, migration, invasion, apoptosis, and metastatic potential. Although it is still an evolving research, recent experimental evidence also suggests that ECS can modulate the functional behaviour of several components of the TME, above all the immune cells, endothelial cells and stromal components. However, the role of ECS in the tumour:stroma interplay remains unclear and research in this area is particularly intriguing. This review aims to shed light on the latest relevant findings of the tumour response to ECS modulation, encouraging a more in-depth analysis in this field. Novel discoveries could be promising for novel anti-tumour approaches, targeting the microenvironmental components and the supportive tumour:stroma crosstalk, thereby hindering tumour development.
The Role of Serum Th1, Th2, and Th17 Cytokines in Patients with Alopecia Areata: Clinical Implications
Authors: Waśkiel-Burnat Anna , Osińska Marta , Salińska Anna , Blicharz Leszek , Goldust Mohamad , Olszewska Małgorzata , Rudnicka Lidia
Published: December 2021

Alopecia areata is a type of non-scarring hair loss. The dysregulation of numerous systemic Th1 (IL-2, IFN-γ, TNF, IL-12, and IL-18), Th2 (IL-4, IL-5, IL-6, IL-9, IL-10, IL-13, IL-17E, IL-31 and IL-33) and Th17 (IL-17, IL-17F, IL-21, IL-22, IL-23 and TGF-β) cytokines was observed in patients with alopecia areata. Positive correlations between the severity of alopecia areata and an increased serum level of various cytokines including IL-2, TNF, IL-12, IL-17, and IL-17E were reported in the literature. An increased serum level of numerous cytokines, such as IL-2, IL-6, TNF, IL-12, IL-17E, and IL-22, was described as positively correlated with the duration of the disease. Moreover, it was shown that increased pre-treatment serum level of IL-12 was a positive, while increased serum levels of IL-4 and IL-13 were negative prognostic markers for the efficacy of diphenylcyclopropenone. In conclusion, alopecia areata is associated with the dysregulation of systemic Th1, Th2 and Th17 cytokines with their role in the pathogenesis, clinical manifestations and prognosis of the disease. Available data indicate the most significant role of serum IL-2, TNF, IL-12, IL-17, and IL-17E as markers of disease activity. The serum levels IL-4, IL-12 and IL-13 may be useful as potential predictors of diphenylcyclopropenone efficacy.
Synthesis of Optically Active Bicyclic Derivatives of Nitroimidazoles
Authors: Żwawiak Justyna , Zaprutko Lucjusz
Published: December 2021

Nitroimidazoles are characterized by a wide range of biological activity and many of them are used as therapeutics. Moreover, some bicyclic nitroimidazooxazoles show considerable potency against Mycobacterium tuberculosis. Some authors noticed that in the case of chiral derivatives of nitroimidazodihydrooxazoles, the (R) form shows a greater tuberculostatic activity than the (S) enantiomer. This work describes the procurement of new 12 enantiomeric bicyclic derivatives of nitroimidazole.
Video-Based Deep Learning Approach for 3D Human Movement Analysis in Institutional Hallways: A Smart Hallway
Authors: McGuirk Connor J. C. , Baddour Natalie , Lemaire Edward D.
Published: December 2021

New artificial intelligence- (AI) based marker-less motion capture models provide a basis for quantitative movement analysis within healthcare and eldercare institutions, increasing clinician access to quantitative movement data and improving decision making. This research modelled, simulated, designed, and implemented a novel marker-less AI motion-analysis approach for institutional hallways, a Smart Hallway. Computer simulations were used to develop a system configuration with four ceiling-mounted cameras. After implementing camera synchronization and calibration methods, OpenPose was used to generate body keypoints for each frame. OpenPose BODY25 generated 2D keypoints, and 3D keypoints were calculated and postprocessed to extract outcome measures. The system was validated by comparing ground-truth body-segment length measurements to calculated body-segment lengths and ground-truth foot events to foot events detected using the system. Body-segment length measurements were within 1.56 (SD = 2.77) cm and foot-event detection was within four frames (67 ms), with an absolute error of three frames (50 ms) from ground-truth foot event labels. This Smart Hallway delivers stride parameters, limb angles, and limb measurements to aid in clinical decision making, providing relevant information without user intervention for data extraction, thereby increasing access to high-quality gait analysis for healthcare and eldercare institutions.
Analysis of Ficus hirta Fig Endosymbionts Diversity and Species Composition
Authors: Liu Yifeng , Fan Songle , Yu Hui
Published: December 2021

Endosymbionts living in plants and insects are pervasive. Ficus (Moraceae) has very special inflorescences (which we also call figs) enclosed like an urn, and such inflorescence is usually parasitized by fig wasps. Ficus breeds fig wasp larvae in its figs and adult fig wasps pollinate for Ficus, Ficus and its obligated pollinator formed fig-fig wasp mutualism. Previous studies have found that this confined environment in figs may have provided protection for fig wasps and that this has left some imprints on the genome of fig wasps during the coevolution history of figs and fig wasps. Research on the diversity of both bacteria and fungi in figs are fewer. Our study explored the diversity of endosymbionts in Ficus hirta figs. We utilized high-throughput sequencing and biological database to identify the specific microorganism in figs, then conducted microorganism communities’ diversity analysis and function annotation analysis. As a result, we identified the dominant endosymbionts in figs, mainly some insect internal parasitic bacteria and fungi, plant pathogen, endophytes, and saprotroph. Then we also found bacteria in Ficus hirta figs were more diversified than fungi, and bacteria communities in female figs and functional male figs were different. These findings may give us more insight into the coevolution and interaction among endosymbiont, fig, and fig wasp.
Ability to Participate in Social Activities of Rheumatoid Arthritis Patients Compared with Other Rheumatic Diseases: A Cross-Sectional Observational Study
Authors: Cano-García Laura , Mena-Vázquez Natalia , Manrique-Arija Sara , Redondo-Rodriguez Rocío , Romero-Barco Carmen María , Fernández-Nebro Antonio
Published: December 2021

Objectives: To compare the ability to participate in social activities among rheumatoid arthritis patients with other rheumatic disease patients and identify potentially implicated factors. Patients and methods: Between June and November 2019, we consecutively selected patients aged ≥18 years with RA (defined according to ACR/EULAR 2010), SpA (ASAS/EULAR 2010), and SLE (ACR 1997). Main outcome measures: Ability to participate in social roles and activities evaluated using the PROMIS score v2.0 short-form 8a (PROMIS-APS). Secondary outcomes: Participation in social activities according to a series of variables (mobility, depression, satisfaction with social relationships, social isolation, company, emotional support, instrumental support, and support via information). We evaluated the association between the ability to participate in social activities and associated variables using multivariable linear regression analysis. Results: The study population comprised 50 patients with RA (33.1%), 51 patients (33.8%) with SpA, and 50 patients (33.1%) with SLE. The mean PROMIS-APS scores were similar in the three groups. The multivariable analysis for the whole sample showed that the ability to participate in social activities was inversely associated with depression and directly with social satisfaction, mobility, company, and age. The stratified analysis revealed an inverse association between inflammatory activity and ability to participate in social activities in patients with RA and SpA, but not in those with SLE. Conclusion: All patients with RA, SpA, and SLE had a similar ability to participate in social activities. This was associated with other psychosocial factors (social satisfaction, mobility, company, depression) and clinical factors (age and inflammatory activity).
Retrospective Change-Points Detection for Multidimensional Time Series of Arbitrary Nature: Model-Free Technology Based on the ϵ-Complexity Theory
Authors: Piryatinska Alexandra , Darkhovsky Boris
Published: December 2021

We consider a retrospective change-point detection problem for multidimensional time series of arbitrary nature (in particular, panel data). Change-points are the moments at which the changes in generating mechanism occur. Our method is based on the new theory of ϵ-complexity of individual continuous vector functions and is model-free. We present simulation results confirming the effectiveness of the method.
Hyperledger Healthchain: Patient-Centric IPFS-Based Storage of Health Records
Authors: Mani Vinodhini , Manickam Prakash , Alotaibi Youseef , Alghamdi Saleh , Khalaf Osamah Ibrahim
Published: December 2021

Blockchain-based electronic health system growth is hindered by privacy, confidentiality, and security. By protecting against them, this research aims to develop cybersecurity measurement approaches to ensure the security and privacy of patient information using blockchain technology in healthcare. Blockchains need huge resources to store big data. This paper presents an innovative solution, namely patient-centric healthcare data management (PCHDM). It comprises the following: (i) in an on-chain health record database, hashes of health records are stored as health record chains in Hyperledger fabric, and (ii) off-chain solutions that encrypt actual health data and store it securely over the interplanetary file system (IPFS) which is the decentralized cloud storage system that ensures scalability, confidentiality, and resolves the problem of blockchain data storage. A security smart contract hosted through container technology with Byzantine Fault Tolerance consensus ensures patient privacy by verifying patient preferences before sharing health records. The Distributed Ledger technology performance is tested under hyper ledger caliper benchmarks in terms of transaction latency, resource utilization, and transaction per second. The model provides stakeholders with increased confidence in collaborating and sharing their health records.
Optimal Hybrid PV Array Topologies to Maximize the Power Output by Reducing the Effect of Non-Uniform Operating Conditions
Authors: Raju Pendem Suneel , Mikkili Suresh , Rangarajan Shriram S. , Avv Sudhakar , Collins Randolph E. , Senjyu Tomonobu
Published: December 2021

The photovoltaic (PV) system center inverter architecture comprises various conventional array topologies such as simple-series (S-S), parallel (P), series-parallel (S-P), total-cross-tied (T-C-T), bridge-linked (B-L), and honey-comb (H-C). The conventional PV array topologies under non-uniform operating conditions (NUOCs) produce a higher amount of mismatching power loss and represent multiple maximum-power-points (M-P-Ps) in the output characteristics. The performance of T-C-T topology is found superior among the conventional topologies under NUOCs. However, T-C-T topology’s main limitations are higher redundancy, more number of electrical connections, higher cabling loss, poor performance during row-wise shading patterns, and more number of switches and sensors for the re-configuration of PV modules. This paper proposes the various optimal hybrid PV array topologies to overcome the limitations of conventional T-C-T array topology. The proposed hybrid topologies are such as series-parallel-cross-tied (S-P-C-T), bridge-link-cross-tied (B-L-C-T), honey-comb-cross-tied (H-C-C-T), series-parallel-total-cross-tied (S-P-T-C-T), bridge-link-total-cross-tied (B-L-T-C-T), honey-comb-total-cross-tied (H-C-T-C-T), and bridge-link-honey-comb (B-L-H-C). The proposed hybrid topologies performance is evaluated and compared with the conventional topologies under various NUOCs. The parameters used for the comparative study are open-circuit voltage, short-circuit current, global-maximum-power-point (GMPP), local-maximum-power-point (LMPP), number of LMPPs, and fill factor (FF). Furthermore, the mismatched power loss and the conversion efficiency of conventional and hybrid array topologies are also determined. Based on the results, it is found that the hybrid array topologies maximize the power output by mitigating the effect of NUOCs and reducing the number of LMPPs.
Empirical Estimation of the Energy Impacts of Projects Installed through Residential Property Assessed Clean Energy Financing Programs in California
Authors: Deason Jeff , Murphy Sean , Goldman Charles A.
Published: December 2021

We examine the energy use impacts of energy efficiency and solar PV projects financed by residential property assessed clean energy (R-PACE) programs in California. We leverage household-level interval meter data to apply normalized metered energy consumption (NMEC) methods at significant scale—more than 25,000 electric meters and more than 15,000 gas meters. We develop a comparison group to account for non-project-related changes in usage. The projects include homes that replaced existing HVAC equipment with higher-efficiency units and homes that installed central heating or air conditioning equipment for the first time. We have limited information on pre-project household equipment stock so we develop a method to infer new installations. We find that projects that installed energy efficiency technologies reduce electricity consumption by approximately 3% and gas consumption by approximately 3.5% on average. When we remove homes that installed new cooling and heating equipment for the first time, savings rise to approximately 5% for electricity and approximately 6% for gas. Given the California climate and the results of an existing study of similar California projects, these results are in line with expectations. Solar PV projects produce electricity that offsets approximately 69% of household electricity consumption on average. We estimate that California R-PACE projects installed through the end of 2019 produce annual reductions in grid-tied electricity consumption of 506 GWh (equivalent to the electricity consumption of approximately 74,000 California households) and gas consumption reductions of 2 million therms (equivalent to the gas consumption of approximately 4700 California households) in a typical weather year.
Modeling, Simulation, and Analysis of a Variable-Length Pendulum Water Pump
Authors: Yakubu Godiya , Olejnik Paweł , Awrejcewicz Jan
Published: December 2021

Due to the long-term problem of electricity and potable water in most developing and undeveloped countries, predominantly rural areas, a novelty of the pendulum water pump, which uses a vertically excited parametric pendulum with variable-length using a sinusoidal excitation as a vibrating machine, is presented. With this, more oscillations can be achieved, reducing human effort further and having high output than the existing pendulum water pump with the conventional pendulum. The pendulum, lever, and piston assembly are modeled by a separate dynamical system and then joined into the many degrees-of-freedom dynamical systems. The present work includes friction while studying the system dynamics and then simulated to verify the system’s harmonic response. The study showed the effect of the pendulum length variability on the whole system’s performance. The vertically excited parametric pendulum with variable length in the system is established, giving faster and longer oscillations than the pendulum with constant length. Hence, more and richer dynamics are achieved. A quasi-periodicity behavior is noticed in the system even after 50 s of simulation time; this can be compensated when a regular external forcing is applied. Furthermore, the lever and piston oscillations show a transient behavior before it finally reaches a stable behavior.
Simplified Thermal Performance Evaluation of a PCM-Filled Triple-Glazed Window under Arctic Climate Conditions
Authors: Ravasio Lucrezia , Calay Rajnish Kaur , Riise Raymond
Published: December 2021

This paper evaluates the thermal performance of a triple-glazed glass window filled with a phase-change material (PCM) compared to the performance of a traditional triple-glazed window with air gaps. The chosen PCM was paraffin wax. A mathematical model to simulate heat transfer within the system was presented. A commercially available software, COMSOL Multiphysics, was used to numerically solve the governing equations. The analysis was carried out for the representative days of different seasons using three types of paraffin wax (5, 10, and 15) that have different melting-temperature ranges. Particularly, the study considers the unique climatic conditions of the Arctic region. Results showed that by integrating a PCM into the cavity of triple-glazing, thermal performance during summer season of the window was enhanced, while for spring and autumn thermal performance was affected by the type of paraffin selected. The thermal performance of glass windows during winter did not change with PCM integration.
Analysis of the Physicochemical Characteristics of Biochar Obtained by Slow Pyrolysis of Nut Shells in a Nitrogen Atmosphere
Authors: Gorshkov Alexander , Berezikov Nikolay , Kaltaev Albert , Yankovsky Stanislav , Slyusarsky Konstantin , Tabakaev Roman , Larionov Kirill
Published: December 2021

The process of slow pyrolysis of seven nut shell samples, in a nitrogen-purged atmosphere, has been studied, as well as characteristics of biochar obtained. The heat carrier with a temperature of 400–600 °C (with a step of 100 °C) was supplied indirectly using a double-walled reactor. The heating rate was 60 °C/min. At increased temperature of the heating medium, a decrease in the amount of the resulting carbon residue averaged 6.2 wt%. The release of non-condensable combustible gas-phase compounds CO, CH4, and H2, with maximum concentrations of 12.7, 14.0, and 0.7 vol%, respectively, was registered. The features of the obtained biochar sample conversions were studied using thermal analysis in inert (nitrogen) and oxidative (air) mediums at 10 °C/min heating rate. Kinetic analysis was performed using Coats–Redfern method. Thermal analysis showed that the main weight loss (Δm = 32.8–43.0 wt%) occurs at temperatures ranging between 290 °C and 400 °C, which is due to cellulose decomposition. The maximum carbon content and, hence, heat value were obtained for biochars made from macadamia nut and walnut shells. An increased degree of coalification of the biochar samples affected their reactivity and, in particular, caused an increase in the initial temperature of intense oxidation (on average, by 73 °C). While technical and elemental composition of nut shell samples studied were quite similar, the morphology of obtained biochar was different. The morphology of particles was also observed to change as the heating medium temperature increased, which was expressed in the increased inhomogeneity of particle surface. The activation energy values, for biochar conversion in an inert medium, were found to vary in the range of 10–35 kJ/mol and, in an oxidative medium—50–80 kJ/mol. According to literature data, these values were characteristic for lignin fibers decomposition and oxidation, respectively.
First Report of Entomopathogenic Fungi Occurrence in Forest Soils in Croatia
Authors: Kovač Marta , Tkaczuk Cezary , Pernek Milan
Published: December 2021

Entomopathogenic fungi (EPF) in Croatian forests are known only from observations of insect cadavers that show obvious signs of disease. To date, their presence in soils has not been investigated. The aim of this study was to investigate their occurrence, diversity, and distribution, and to assess their density in tested soils. Soil samples were collected during 2018, 2019, and 2020 at different localities throughout the country, and analyzed by using a method of isolation of fungi on selective culture media. To assess the density of EPF in tested soils, colonies of individual fungal species were counted and recorded; the results were expressed as the number of colony-forming units (CFU) per gram of dry soil. After morphological and molecular analysis, five entomopathogenic fungal genera were identified: Beauveria spp., Metarhizium spp., Purpureocillium spp., Lecanicillium spp., and Paecilomyces spp. Results also showed that the range of a total EPF colony density in the soil varies from 4 × 103 to 27.4 × 103 CFU g−1. The most common were EPF of the genus Beauveria, which were recorded at four of five locations, and at 16 of 25 sampling points, but the highest average number (density) of colonies belonged to the genus Metarhizium. Since this type of research was never conducted in Croatia previously, this is the first evidence that insect pathogenic fungi are present in soils of different natural forest habitats. Such research can be useful in selecting and utilizing entomopathogens that are suitable for biological pest control in certain target areas.
Research on the Consumption Trend, Nutritional Value, Biological Activity Evaluation, and Sensory Properties of Mini Fruits and Vegetables
Authors: Wang Jiaqi , Ma Tingting , Wang Lukai , Lan Tian , Fang Yulin , Sun Xiangyu
Published: December 2021

Mini fruits and vegetables (MFV) are pocket fruits and vegetables whose shape and volume are significantly smaller than those widely sold and well-known normal fruits and vegetables (NFV) on the market. Through the research on the market status and consumption trends of MFV, it was found that MFV have recently become a new market favorite. However, compared with NFV, there was found to be no relevant data on sensory quality, nutritional value, safety, etc. of MFV; this could indicate low consumer awareness of MFV, which in turn affects their planting and sales choices, as well as the market scale remaining small. In this context, six MFV with high degree of marketization were selected and compared with their corresponding NFV to evaluate the nutritional value, biological activity, and sensory properties. The results showed the nutritional value of MFV to be mainly related to their species. The nutritional value of MFV derived from immature, tender vegetables was generally lower than that of mature NFV. For example, the content of zeaxanthin in normal maize was 0.43 mg/kg, which was about 2.87 times that of mini maize (0.15 mg/kg). For newly cultivated mini varieties, their nutritional value often had different trends and rules compared with NFV. The nutritional value obtained by consuming MFV is not equal to that obtained by consuming the corresponding NFV of the same weight.
Physicochemical, Sensory and Nutritional Properties of Foods Affected by Processing and Storage
Authors: Martínez Sidonia , Carballo Javier
Published: December 2021

Due to their chemical composition and physicochemical characteristics, most foods are very perishable and are easily altered by physical, chemical and biological agents [...]
Machine Learning and Bioinformatics Framework Integration to Potential Familial DCM-Related Markers Discovery
Authors: Schiano Concetta , Franzese Monica , Geraci Filippo , Zanfardino Mario , Maiello Ciro , Palmieri Vittorio , Soricelli Andrea , Grimaldi Vincenzo , Coscioni Enrico , Salvatore Marco , Napoli Claudio
Published: December 2021

Objectives: Dilated cardiomyopathy (DCM) is characterized by a specific transcriptome. Since the DCM molecular network is largely unknown, the aim was to identify specific disease-related molecular targets combining an original machine learning (ML) approach with protein-protein interaction network. Methods: The transcriptomic profiles of human myocardial tissues were investigated integrating an original computational approach, based on the Custom Decision Tree algorithm, in a differential expression bioinformatic framework. Validation was performed by quantitative real-time PCR. Results: Our preliminary study, using samples from transplanted tissues, allowed the discovery of specific DCM-related genes, including MYH6, NPPA, MT-RNR1 and NEAT1, already known to be involved in cardiomyopathies Interestingly, a combination of these expression profiles with clinical characteristics showed a significant association between NEAT1 and left ventricular end-diastolic diameter (LVEDD) (Rho = 0.73, p = 0.05), according to severity classification (NYHA-class III). Conclusions: The use of the ML approach was useful to discover preliminary specific genes that could lead to a rapid selection of molecular targets correlated with DCM clinical parameters. For the first time, NEAT1 under-expression was significantly associated with LVEDD in the human heart.
A Comprehensive Survey of Statistical Approaches for Differential Expression Analysis in Single-Cell RNA Sequencing Studies
Authors: Das Samarendra , Rai Anil , Merchant Michael L. , Cave Matthew C. , Rai Shesh N.
Published: December 2021

Single-cell RNA-sequencing (scRNA-seq) is a recent high-throughput sequencing technique for studying gene expressions at the cell level. Differential Expression (DE) analysis is a major downstream analysis of scRNA-seq data. DE analysis the in presence of noises from different sources remains a key challenge in scRNA-seq. Earlier practices for addressing this involved borrowing methods from bulk RNA-seq, which are based on non-zero differences in average expressions of genes across cell populations. Later, several methods specifically designed for scRNA-seq were developed. To provide guidance on choosing an appropriate tool or developing a new one, it is necessary to comprehensively study the performance of DE analysis methods. Here, we provide a review and classification of different DE approaches adapted from bulk RNA-seq practice as well as those specifically designed for scRNA-seq. We also evaluate the performance of 19 widely used methods in terms of 13 performance metrics on 11 real scRNA-seq datasets. Our findings suggest that some bulk RNA-seq methods are quite competitive with the single-cell methods and their performance depends on the underlying models, DE test statistic(s), and data characteristics. Further, it is difficult to obtain the method which will be best-performing globally through individual performance criterion. However, the multi-criteria and combined-data analysis indicates that DECENT and EBSeq are the best options for DE analysis. The results also reveal the similarities among the tested methods in terms of detecting common DE genes. Our evaluation provides proper guidelines for selecting the proper tool which performs best under particular experimental settings in the context of the scRNA-seq.
Antioxidant and Anti-Obesity Potentials of Korean-Native Wild Vegetables (Allium species)
Authors: Lee Ji Yeon , Seo Kyung Hye , Lee Eun Young , Ji Yun-Jeong , Lee Yun Ji , Kang Min Hye , Seong Hyun-A , Kim Hyung Don
Published: December 2021

Four Korea-native wild vegetables belonging to the Allium genus (Allium tuberosum Rottl., Allium senescens L., Allium thunbergii G. Don., and Allium sacculiferum Maxim.) were investigated for their antioxidant and anti-obesity potential, in order to promote the utilization of wild edible plants for the development of potential new crops. In this study, we compared the antioxidant activity of the plant extracts by ABTS+ and DPPH scavenging method. We then compared the anti-adipogenic effects of the extracts in adipocytes. We quantified the phenolics in the extracts using high-performance liquid chromatography (HPLC) to identify the bioactive compounds. A. tuberosum and A. sacculiferum had a higher total phenolic content (19.0 ± 0.3 and 19.6 ± 0.7 mg GA/g extract, respectively) than A. senescens and A. thunbergii (16.0 ± 0.2 and 14.5 ± 0.7 mg GA/g extract). Caffeic acid, an antioxidant and anti-adipogenic compound, was detected in A. tuberosum and A. sacculiferum, but not in A. senescens and A. thunbergii. A. tuberosum, and A. sacculiferum showed superior antioxidative and anti-adipogenic activities compared to A. senescens and A. thunbergii. Therefore, A. tuberosum and A. sacculiferum, both of which contain antioxidant and anti-adipogenic phenolics, such as caffeic acid, present a therapeutic potential against obesity. Various phenolic compounds, which have been found to differ among Allium spp., may partially account for differences among the Allium spp. with respect to their antioxidant and anti-obesity effects.
Trans*Forming Access and Care in Rural Areas: A Community-Engaged Approach
Authors: Gandy Megan E. , Kidd Kacie M. , Weiss James , Leitch Judith , Hersom Xavier
Published: December 2021

Research indicates that rural transgender and gender diverse (TGD) populations have a greater need for health services when compared with their urban counterparts, face unique barriers to accessing services, and have health disparities that are less researched than urban TGD populations. Therefore, the primary aim of this mixed-methods study (n = 24) was to increase research on the health care needs of TGD people in a rural Appalachian American context. This study was guided by a community-engaged model utilizing a community advisory board of TGD people and supportive parents of TGD children. Quantitative results indicate that travel burden is high, affirming provider availability is low, and the impacts on the health and mental health of TGD people in this sample are notable. Qualitative results provide recommendations for providers and health care systems to better serve this population. Integrated mixed-methods results further illustrate ways that rural TGD people and families adapt to the services available to them, sometimes at significant economic and emotional costs. This study contributes to the small but growing body of literature on the unique needs of rural TGD populations, including both adults and minors with supportive parents, by offering insights into strategies to address known disparities.