bluuebunny/crossref_metadata_2025 huggingface dataset:
Finite-Time Controller Design for the Dynamic Positioning of Ships Considering Disturbances and Actuator Constraints
Authors: Zhang Yufang , Liu Changde , Zhang Nan , Ye Qian , Su Weifeng
Published: July 2022

Focusing on dynamic positioning (DP) systems for ships, which are subject to environmental disturbances and actuator constraints, this paper presents a finite-time controller that uses a disturbance observer with the aid of a backstepping technique. First, to estimate the time-varying and unknown environmental disturbances in finite time, two sliding-mode disturbance observers are constructed. Specifically, an adaptive disturbance observer (ADO) effectively decreases undesired chattering without the need for prior information on environmental disturbances. Then, to handle the actuator constraints, the designed control forces are distributed into multiple actuators using a control allocation algorithm to obtain the actual forces. Next, an auxiliary dynamic system is built to compensate for velocity tracking errors induced by the mismatch of the DP control law and thruster forces. Then, with the designed ADO and the auxiliary dynamic system, a finite-time controller with a fast exponential-reaching law is designed; this ensures that the positioning errors and the sliding surface converge to zero at a fast convergence rate. Finally, numerical simulations are presented: these present a cable-laying ship experiencing wind, currents, and waves in different sea states. The results show the effectiveness of the presented control scheme.
Analytical Performance of the Commercial MucorGenius® Assay as Compared to an In-House qPCR Assay to Detect Mucorales DNA in Serum Specimens
Authors: Ghelfenstein-Ferreira Théo , Verdurme Laura , Alanio Alexandre
Published: July 2022

Standardized, reproducible and validated Mucorales quantitative PCR (qPCR) assays are needed in the context of routine testing in diagnostic labs. We, therefore, compared the commercial MucorGenius® assay (PathoNostics, Maastricht) targeting five genera of Mucorales to our in-house qPCR targeting Rhizomucor spp., Lichtheimia spp. and Mucor/Rhizopus spp. To assess their analytical sensitivity, 25 frozen leftover serum specimens, which had already tested positive based on our in-house assay, were selected. These sera were from 15 patients with probable or proven mucormycosis. For analytical specificity, 0.5 pg from 15 purified fungal DNAs from nine different Mucorales genera were spiked into pooled qPCR-negative leftover serum specimens. All samples were tested in parallel with both assays and the quantitative cycles (Cq) were compared. A total of 13/25 (52%) serum samples were amplified by one of the two assays with only four of them detected with the MucorGenius® assay. In spiked specimens, all targeted strains were successfully amplified by our in-house qPCR. The MucorGenius® assay was not able to detect Lichtheimia corymbifera but successfully amplified all other species targeted by the kit and two additional non-targeted species (Syncephalastrum monosporum and Saksenaea vasiformis). The MucorGenius® assay showed lower analytical sensitivity compared to our in-house assay. Indeed, the MucorGenius® assay amplified more species, as expected, but showed a decreased detection of the frequent species Lichtheimia corymbifera.
Emergence and Genomic Characterization of Multidrug Resistant Candida auris in Nigeria, West Africa
Authors: Oladele Rita , Uwanibe Jessica N. , Olawoye Idowu B. , Ettu Abdul-Wahab O. , Meis Jacques F. , Happi Christian T.
Published: July 2022

Candida auris is an emerging multidrug-resistant fungal pathogen that has become a worldwide public health threat due to the limitations of treatment options, difficulty in diagnosis, and its potential for clonal transmission. Four ICU patients from three different healthcare facilities in Southern Nigeria presented features suggestive of severe sepsis and the blood cultures yielded the growth of Candida spp., which was identified using VITEK 2 as C. auris. Further confirmation was performed using whole genome sequencing (WGS). From the genomic analysis, two had mutations that conferred resistance to the antifungal azole group and other non-synonymous mutations in hotspot genes, such as ERG2, ERG11, and FKS1. From the phylogenetic analysis, cases 2 and 4 had a confirmed mutation (ERG11:Y132F) that conferred drug resistance to azoles clustered with clade 1, whilst cases 1 and 3 clustered with clade 4. Three of the patients died, and the fourth was most likely a case of colonization since he received no antifungals and was discharged home. These first cases of C. auris reported from Nigeria were most likely introduced from different sources. It is of public health importance as it highlights diagnostic gaps in our setting and the need for active disease surveillance in the region.
High Neutrophil–Lymphocyte Ratio and Low Lymphocyte–Monocyte Ratio Combination after Thrombolysis Is a Potential Predictor of Poor Functional Outcome of Acute Ischemic Stroke
Authors: Sadeghi Farzaneh , Sarkady Ferenc , Zsóri Katalin S. , Szegedi István , Orbán-Kálmándi Rita , Székely Edina G. , Vasas Nikolett , Berényi Ervin , Csiba László , Bagoly Zsuzsa , Shemirani Amir H.
Published: July 2022

(1) Background: Ischemic stroke is one of the leading causes of death and disability. An inflammatory response is observed in multiple stages of cerebral ischemia, particularly in the acute phase. Recent publications revealed that the neutrophil–lymphocyte ratio (NLR) and lymphocyte–monocyte ratio (LMR) may be used to predict long-term prognosis in acute ischemic stroke (AIS) after thrombolysis. To test whether there is a relationship between the combination of these parameters and long-term prognosis, we analyzed the NLR–LMR combination in AIS patients treated with intravenous recombinant tissue plasminogen activator (rtPA); (2) Methods: The study included 285 adults with a diagnosis of AIS and rtPA treatment within a 4.5 h time window. Blood samples were obtained at admission and 24 h after thrombolysis to calculate pre- and post-thrombolysis NLR and LMR. Clinical data, including NIHSS was registered on admission and day 1. The long-term outcome was defined 90 days post-event by the modified Rankin Scale (mRS). Therapy-associated intracranial hemorrhage (ICH) was classified according to ECASS II. Receiver operating characteristic curve (ROC) analysis was performed to determine optimal cutoffs of NLR and LMR as predictors of therapy outcomes; (3) Results: Patients were stratified by cutoffs of 5.73 for NLR and 2.08 for LMR. The multivariate logistic regression model, including all possible confounders, displayed no significant association between NLR or LMR with 3-months functional prognosis. The combination of high NLR–low LMR vs. low NRL–high LMR as obtained 24 h after thrombolysis was found to be an independent predictor of poor 3-months functional outcome (mRS ≥ 2; OR 3.407, 95% CI 1.449 to 8.011, p = 0.005). The proportion of patients between low NLR–high LMR and high NLR–low LMR groups from admission to day 1 showed no significant change in the good outcome group. On the other hand, in the poor outcome group (mRS ≥ 2), low NLR–high LMR and high NLR–low LMR groups displayed a significant shift in patient proportions from 67% and 21% at admission (p = 0.001) to 36% and 49% at 24 h after thrombolysis (p < 0.001), respectively; (4) Conclusions: Our study demonstrated for the first time that a high NLR–low LMR combination as observed at 24 h after thrombolysis can serve as an independent predictor of 3-months poor outcome in AIS patients. This simple and readily available data may help clinicians to improve the prognostic estimation of patients and may provide guidance in selecting patients for personalized and intensified care post-thrombolysis.
Using a Clinical Formulation to Understand Psychological Distress in People Affected by Huntington’s Disease: A Descriptive, Evidence-Based Model
Authors: Dale Maria , Wood Ashleigh , Zarotti Nicolò , Eccles Fiona , Gunn Sarah , Kiani Reza , Mobley Amanda , Robertson Noelle , Simpson Jane
Published: July 2022

Huntington’s disease (HD) is an inherited, life-limiting neurodegenerative condition. People with HD experience changes in cognitive, motor and emotional functioning, and can also, mainly at later stages, exhibit behaviours that professionals and carers might find distressing such as hitting others, throwing objects, swearing or making inappropriate comments. While clinical formulation (an individualised approach used by mental health professionals to describe an individual’s difficulties) is a helpful tool to conceptualise patients’ wellbeing, a specific formulation framework has not yet been developed for HD. However, evidence has shown that formulation can help guide clinical interventions and increase consistency of approach across multi-disciplinary teams, refine risk management, and improve staff or carers’ empathic skills and understanding of complex presentations. As a consequence, this paper proposes a new clinical formulation model for understanding distress among people with HD, based on a biopsychosocial framework. More specifically, this includes key elements centring on an individual’s past experience and personal narratives, as well as anticipatory cognitions and emotions about the future. In-depth discussions regarding the components of the model and their importance in HD formulations are included, and a fictional yet representative case example is presented to illustrate their application within the context of personalised care.
Real-Life Experience on Directional Deep Brain Stimulation in Patients with Advanced Parkinson’s Disease
Authors: Koivu Maija , Scheperjans Filip , Eerola-Rautio Johanna , Vartiainen Nuutti , Resendiz-Nieves Julio , Kivisaari Riku , Pekkonen Eero
Published: July 2022

Directional deep brain stimulation (dDBS) is preferred by patients with advanced Parkinson’s disease (PD) and by programming neurologists. However, real-life data of dDBS use is still scarce. We reviewed the clinical data of 53 PD patients with dDBS to 18 months of follow-up. Directional stimulation was favored in 70.5% of dDBS leads, and single segment activation (SSA) was used in 60% of dDBS leads. Current with SSA was significantly lower than with other stimulation types. During the 6-month follow-up, a 44% improvement in the Unified Parkinson’s Disease Rating Scale (UPDRS-III) points and a 43% decline in the levodopa equivalent daily dosage (LEDD) was observed. After 18 months of follow-up, a 35% LEDD decrease was still noted. The Hoehn and Yahr (H&Y) stages and scores on item no 30 “postural stability” in UPDRS-III remained lower throughout the follow-up compared to baseline. Additionally, dDBS relieved non-motor symptoms during the 6 months of follow-up. Patients with bilateral SSA had similar clinical outcomes to those with other stimulation types. Directional stimulation appears to effectively reduce both motor and non-motor symptoms in advanced PD with minimal adverse effects in real-life clinical care.
The Role of the Selected miRNAs as Diagnostic, Predictive and Prognostic Markers in Non-Small-Cell Lung Cancer
Authors: Szczyrek Michał , Bitkowska Paulina , Jutrzenka Marta , Milanowski Janusz
Published: July 2022

Lung cancer remains a leading cause of cancer-related deaths worldwide, overtaking colon, breast, and prostate cancer-related deaths. Due to the limited diagnostic possibilities, it is often diagnosed after it has reached an advanced stage. The delayed diagnosis significantly worsens the patient’s prognosis. In recent years, we have observed an increased interest in the use of microRNAs (miRNAs) as diagnostic, predictive, and prognostic markers in non-small-cell lung cancer (NSCLC). The abnormal expression levels of the miRNAs could be used to detect NSCLC in its early stages while it is still asymptomatic. This could drastically improve the clinical outcome. Furthermore, some miRNAs could serve as promising predictive and prognostic factors for NSCLC. Some of the currently available studies have shown a correlation between the miRNAs’ levels and the sensitivity of tumour cells to different treatment regimens. Analysing and modulating the miRNAs’ expression could be a way to predict and improve the treatment’s outcome.
Enhancing Anticoagulation Monitoring and Therapy in Patients Undergoing Microvascular Reconstruction in Maxillofacial Surgery: A Prospective Observational Trial
Authors: Schröder Tom A. , Leonhardt Henry , Haim Dominik , Bräuer Christian , Papadopoulos Kiriaki K. , Vicent Oliver , Güldner Andreas , Mirus Martin , Schmidt Jürgen , Held Hanns C. , Tiebel Oliver , Birkner Thomas , Beyer-Westendorf Jan , Lauer Günter , Spieth Peter M. , Koch Thea , Heubner Lars
Published: July 2022

Background: In reconstructive surgery, loss of a microvascular free flap due to perfusion disorders, especially thrombosis, is a serious complication. In recent years, viscoelastic testing (VET) has become increasingly important in point-of-care (POC) anticoagulation monitoring. This paper describes a protocol for enhanced anticoagulation monitoring during maxillofacial flap surgery. Objective: The aim of the study will be to evaluate, in a controlled setting, the predictive value of POC devices for the type of flap perfusion disorders due to thrombosis or bleeding. VET, Platelet monitoring (PM) and standard laboratory tests (SLT) are comparatively examined. Methods/Design: This study is an investigator-initiated prospective trial in 100 patients undergoing maxillofacial surgery. Patients who undergo reconstructive surgery using microvascular-free flaps will be consecutively enrolled in the study. All patients provide blood samples for VET, PM and SLT at defined time points. The primary outcome is defined as free flap loss during the hospital stay. Statistical analyses will be performed using t-tests, including the Bonferroni adjustment for multiple comparisons. Discussion: This study will help clarify whether VET can improve individualized patient care in reconstruction surgery. A better understanding of coagulation in relation to flap perfusion disorders may allow real-time adaption of antithrombotic strategies and potentially prevent flap complications.
Asthma and Tobacco Smoking
Authors: Bellou Vanesa , Gogali Athena , Kostikas Konstantinos
Published: July 2022

Asthma is a prevalent chronic pulmonary condition with significant morbidity and mortality. Tobacco smoking is implicated in asthma pathophysiology, diagnosis, prognosis and treatment. Smokers display increased prevalence and incidence of asthma, but a causal association cannot be claimed using existing evidence. Second-hand smoking and passive exposure to tobacco in utero and early life have also been linked with asthma development. Currently, approximately one-fourth of asthma patients are smokers. Regular smokers with asthma might display accelerated lung function decline and non-reversible airflow limitation, making their distinction from chronic obstructive pulmonary disease patients challenging. Asthma patients who smoke typically have uncontrolled disease, as shown by increased symptoms, more exacerbations and impaired quality of life. On the other hand, smoking cessation improves lung function and asthma severity. Thus, asthma patients and their caregivers should be actively questioned about their smoking status at each medical encounter, and smoking cessation ought to be strongly encouraged both for patients with asthma and their close contacts. Smokers with asthma should be provided with comprehensive smoking cessation interventions on top of other anti-asthma medications.
Single Channel Image Enhancement (SCIE) of White Blood Cells Based on Virtual Hexagonal Filter (VHF) Designed over Square Trellis
Authors: Rasheed Shahid , Raza Mudassar , Sharif Muhammad , Kadry Seifedine , Alharbi Abdullah
Published: July 2022

White blood cells (WBCs) are the important constituent of a blood cell. These blood cells are responsible for defending the body against infections. Abnormalities identified in WBC smears lead to the diagnosis of disease types such as leukocytosis, hepatitis, and immune system disorders. Digital image analysis for infection detection at an early stage can help fast and precise diagnosis, as compared to manual inspection. Sometimes, acquired blood cell smear images from an L2-type microscope are of very low quality. The manual handling, haziness, and dark areas of the image become problematic for an efficient and accurate diagnosis. Therefore, WBC image enhancement needs attention for an effective diagnosis of the disease. This paper proposed a novel virtual hexagonal trellis (VHT)-based image filtering method for WBC image enhancement and contrast adjustment. In this method, a filter named the virtual hexagonal filter (VHF), of size 3 × 3, and based on a hexagonal structure, is formulated by using the concept of the interpolation of real and square grid pixels. This filter is convolved with WBC ALL-IBD images for enhancement and contrast adjustment. The proposed filter improves the results both visually and statically. A comparison with existing image enhancement approaches proves the validity of the proposed work.
Health Insurance Patterns of Older Veterans: Evidence from the Health and Retirement Study
Authors: Stype Amanda C.
Published: July 2022

With the increased availability of community care to veterans from the VA MISSION Act, policymakers and providers need to understand how older veterans are insured, particularly before Medicare eligibility at age 65. Using data from 1996 to 2018, this study examines the insurance patterns of veterans prior to the expansion of access to community care through the VA and compares those patterns to nonveterans. This study finds that veterans are more likely to have insurance than nonveterans and that they are less likely to rely on Medicaid and Medicare before age 65. Regression estimates also suggest that veterans with at least some college education are less likely to have private insurance and are more likely to be uninsured than nonveterans with the same educational attainment.
Assessment on the Urbanization Quality of China’s Main Grain-Producing Areas under the SDGs
Authors: Geng Fengjuan , Zhang Jing , Miao Changhong , Shang Wenying , Yuan Liuyang
Published: July 2022

The Sustainable Development Goals (SDGs) adopted by the United Nations in 2015 guide the important direction of high-quality urbanization in China’s main grain-producing areas (MGPAs), and improving the quality of urbanization is also crucial to achieve the SDGs. China’s MGPAs not only undertake the task of promoting urbanization but also of ensuring food security. The establishment of an index system based on SDGs can more effectively measure the urbanization quality of MGPAs. For the specific targets of the SDGs, this study established two sets of multidimensional indicator systems, whether including the goals of food and agriculture, and tracked the progress toward improving urbanization quality of China’s MGPAs, including 128 prefecture-level cities, during 2010–2018. We found that the comprehensive urbanization quality and the index of economic efficiency, urbanization level, and environmental quality showed an upward trend with significant regional differences and spatial agglomeration distributions, but the level of agricultural development and urban–rural co-ordination have declined in recent years; the ranking and distribution of urbanization quality, including agricultural development, varied significantly, and the number of cities belonging to the good co-ordination mode decreased as some cities changed to a lower level; and urbanization that does not sacrifice the agricultural capabilities of MGPAs could improve urbanization quality and implement the SDGs.
Protected Natural Spaces, Agrarian Specialization and the Survival of Rural Territories: The Cases of Sierra Nevada (Spain) and Alta Murgia (Italy)
Authors: Cejudo-García Eugenio , Labianca Marilena , Navarro-Valverde Francisco , Belliggiano Angelo
Published: July 2022

In Europe today, there is increasing interest in the management of protected spaces, not only in an attempt to ensure their conservation but also because of their enormous potential for promoting rural development. These protected spaces are generally designed from the top down, although, in an increasing number of cases, they are being promoted by rural communities themselves. The situation across Europe with regard to protected areas is extraordinarily complex due, among other reasons, to the variety of categories and types of protected areas at the regional level. The objective of this study was to compare two parks: the Sierra Nevada National and Natural Park in Andalusia, Spain, and the Alta Murgia National Park in Apulia, Italy, in order to identify any similarities and/or differences between them. To this end, we performed a dynamic analysis of the evolution of the crops, uses, and livestock species using a specific indicator that can detect local dynamics by comparing areas inside the parks with those in the immediate surrounding areas. The results pointed, in part, to a resurgence of these places. In both cases, a trend was observed towards more extensive farming of certain crops and livestock species that are more profitable and/or more highly regarded as quality products. In other cases, there was a risk of traditional crops and agricultural landscapes being abandoned and lost. Various threats were identified in relation to capital-intensive forms of agriculture, especially involving greenhouse cultivation on the Mediterranean coast in the provinces of Granada and Almeria.
Challenges for Social Participation in Conservation in the Biocultural Landscape Area in the Western Sierra of Jalisco
Authors: Maldonado Ibarra Oscar Alberto , Chávez-Dagostino Rosa María , Bravo-Olivas Myrna Leticia , Amparán-Salido Rosío T.
Published: July 2022

The protection of biocultural heritage has generated alternative proposals for the conservation of rural areas. Varied organizations collaborate in a pioneering conservation model, the Biocultural Landscape (BL), where local participation is paramount, that operates in the Western Sierra of Jalisco. The objective of this work was to analyze social participation, conditions, and characteristics of the model based on the WWF and IUCN guidelines. Information about the context, management, and planning was collected and synthesized. The data of territorial management, conservation, knowledge, and local conflicts about participatory processes were collected from 12 stakeholders and analyzed with ATLAS.ti software. It was found that, although local people are familiar with the concept of the protected natural area and the BL model, they cannot clearly identify its objective. The most informed are the interested population that collaborates closely. There are conflicts of interest between those who collaborate directly with the BL and those who do not, which have been resolved through the active participation of the different levels of government and experts who have intervened as mediators. Environmental awareness about the importance of conservation has been achieved by integrating the communities. Given that it is not a restrictive protection model and the rules were created in conjunction with the community, local participation is encouraged.
Agricultural Services: Another Way of Farmland Utilization and Its Effect on Agricultural Green Total Factor Productivity in China
Authors: Xu Qinhang , Zhu Peixin , Tang Liang
Published: July 2022

Improving agricultural green total factor productivity (AGTFP) is an important aspect of sustainable agricultural development. Agricultural services, a new way of farmland utilization in agricultural production, solved the problem of ‘who and how to farm’ in the context of labor off-farm migration. The literature has analyzed different factors that affect AGTFP, but there is a relative dearth of research into agricultural services and AGTFP. Therefore, based on the panel data of 31 provinces from 2011 to 2020, this study firstly measured carbon emissions in agricultural production and then took it as an unexpected output to measure the AGTFP by using the global Malmquist–Luenberger (GML) productivity index. Finally, the effect of agricultural services on AGTFP and its decomposition were empirically verified. The main findings are as follows: (1) Between 2011 and 2020, agricultural carbon emissions increased from 85.63 million tons to 90.99 million tons in the first five years and decreased gradually to 78.64 million tons in 2020; the government policy significantly affects carbon emissions reduction. (2) AGTFP has been increasing for the past decade, and the average growth rate of AGTFP reached 1.016, and agricultural services promoted AGTFP growth significantly, in which technological progress was the crucial driving factor. (3) Taking the Heihe–Tengchong line as the demarcation, the improving effect of agricultural services on AGTFP in the eastern region is better than the western region.
Analyzing Resilience in the Greater Yellowstone Ecosystem after the 1988 Wildfire in the Western U.S. Using Remote Sensing and Soil Database
Authors: Li Hang , Speer James H. , Thapa Ichchha
Published: July 2022

The 1988 Yellowstone fire altered the structure of the local forest ecosystem and left large non-recovery areas. This study assessed the pre-fire drivers and post-fire characteristics of the recovery and non-recovery areas and examined possible reasons driving non-recovery of the areas post-fire disturbance. Non-recovery and recovery areas were sampled with 44,629 points and 77,501 points, from which attribute values related to topography, climate, and subsequent soil conditions were extracted. We calculated the 1988 Yellowstone fire burn thresholds using the differenced Normalized Burn Ratio (dNBR) and official fire maps. We used a burn severity map from the US Forest Service to calculate the burn severity values. Spatial regressions and Chi-Square tests were applied to determine the statistically significant characteristics of a lack of recovery. The non-recovery areas were found to cover 1005.25 km2. Among 11 variables considered as potential factors driving recovery areas and 13 variables driving non-recovery areas, elevation and maximum temperature were found to have high Variance Inflation Factors (4.73 and 4.72). The results showed that non-recovery areas all experienced severe burns and were located at areas with steeper slopes (13.99°), more precipitation (871.73 mm), higher pre-fire vegetation density (NDVI = 0.38), higher bulk density (750.03 kg/m3), lower soil organic matter (165.61 g/kg), and lower total nitrogen (60.97 mg/L). Chi-square analyses revealed statistically different pre-fire forest species (p < 0.01) and soil order (p < 0.01) in the recovery and non-recovery areas. Although Inceptisols dominated in both recovery and non-recovery areas, however, the composition of Mollisols was higher in the non-recovery areas (14%) compared to the recovery areas (11%). This indicated the ecological memory of the non-recovery site reverting to grassland post-disturbance. Unlike conventional studies only focusing on recovery areas, this study analyzed the non-recovery areas and found the key characteristics that make a landscape not resilient to the 1988 Yellowstone fire. The significant effects of elevation, precipitation, and soil pH on recovery may be significant to the forest management and forest resilience in the post-fire period.
Analysing Obstacles and Challenges in Fighting Corruption in Cases of Illegal Investments
Authors: Olmos Giupponi Belen , Yu Hong-Lin
Published: July 2022

Due to existing shortcomings in the system, the suitability and effectiveness of the international investment arbitration regime in addressing corrupt practices in international transactions and investment projects has been frequently questioned. The current legal and regulatory regime presumes that there is a level playing field, i.e., that the parties to an arbitration have equal access to information regarding corrupt actions. However, in practice, bringing claims of corruption in international investment fora meets various obstacles such as evidentiary hurdles and the lack of a specific arbitrators’ mandate. Hence, the focus of this article is on addressing gaps in the international investment arbitration regime dealing with corruption cases. There is increasing concern that the international legal and regulatory regime is inadequate and contains gaps that permit multinational firms to engage in illegal acts involving corruption. Against this backdrop, the main issue that arises is how the international community should respond. This article reviews the gaps in the international investment arbitration regime and then identifies two broad strategies to address the issue of accountability. The first strategy would be to build on and strengthen the existing international investment arbitration regime, which would imply its re-engineering. A second strategy would be to establish a regime providing a new forum and an avenue for dedicated international criminal investigators to be paired with dedicated anticorruption courts that would handle criminal complaints. The Anticorruption Protocol to the United Nations Convention against Corruption (APUNCAC) represents an example of the second strategy. The APUNCAC is a model convention that calls for the implementation of a system comprising dedicated international criminal investigators and dedicated anticorruption courts, in addition to a system where plaintiffs could pursue civil class actions and seek treble damages. The APUNCAC represents a more radical strategy for addressing corruption on the international level. In addition, the APUNCAC would also permit civil class actions seeking treble damages. Overall, the APUNCAC would offer claimants an opportunity to pursue their claims in a neutral forum.
The Influence of Dietary Supplementations on Neuropathic Pain
Authors: D’Egidio Francesco , Lombardozzi Giorgia , Kacem Ben Haj M’Barek Housem E. , Mastroiacovo Giada , Alfonsetti Margherita , Cimini Annamaria
Published: July 2022

Neuropathic pain is defined as pain caused by a lesion or disease of the somatosensory nervous system and affects 7–10% of the worldwide population. Neuropathic pain can be induced by the use of drugs, including taxanes, thus triggering chemotherapy-induced neuropathic pain or as consequence of metabolic disorders such as diabetes. Neuropathic pain is most often a chronic condition, and can be associated with anxiety and depression; thus, it negatively impacts quality of life. Several pharmacologic approaches exist; however, they can lead numerous adverse effects. From this perspective, the use of nutraceuticals and diet supplements can be helpful in relieve neuropathic pain and related symptoms. In this review, we discuss how diet can radically affect peripheral neuropathy, and we focus on the potential approaches to ameliorate this condition, such as the use of numerous nutritional supplements or probiotics.
Challenges in the Detection of Clinically Useful Biomarkers for the Diagnosis of Delirium in Older People in the Emergency Department—A Case–Control Study
Authors: Soler-Sanchis Angela , Martínez-Arnau Francisco Miguel , Sánchez-Frutos José , Pérez-Ros Pilar
Published: July 2022

Background: The identification of biomarkers associated with delirium in the emergency department could contribute to the understanding, prediction and diagnosis of this disorder. The present study was carried out to identify biomarkers included in easily and quickly obtained standard blood examinations in older patients with delirium in the emergency department. Methods: A case–control study was carried out in the emergency department of Francesc de Borja Hospital (Gandía, Valencia, Spain). Older adults (≥65 years of age) diagnosed with delirium (n = 128) were included. Cases due to alcohol or substance abuse were excluded. Controls were selected on a randomized basis from the remaining patients (n = 128). All laboratory test parameters included in the routine blood and urine tests of the emergency department were collected. Results: The mean age of the patients was 81.24 ± 7.51 years, and 56.2% were males, while the mean age of the controls was 78.97 ± 7.99 years, and 45.3% were males. Significant differences were found between the cases and controls in relation to the following parameters: urea 43 (32–58) mg/dL versus 50 (37–66) mg/dL, respectively; neutrophils 69.6 (62.05–78.75)% versus 75.5 (65.1–83.2)%; monocytes 8.7 (7–10.4)% versus 7.6 (5.5–9.2)%; platelets 213 (159–266) × 109/L versus 224 (182–289) × 109/L; neutrophil–lymphocyte ratio 3.88 (2.45–7.07) versus 5 (2.75–8.83); platelet–lymphocyte ratio 281.4 (210–360) versus 357.1 (257.8–457.1); and mean platelet volume 10.6 (10–11.5) fl versus 10.4 (9.67–10.9) fl. Although the mean values were above desirable levels in both groups, they were higher for most parameters in the control group. No significant differences were observed in C-reactive protein concentration (9.99 (1.69–51) mg/L versus 12.3 (3.09–65.97) mg/L). Conclusions: The identification of delirium biomarkers poses difficulties due to the urgent nature of the disorders found in older people admitted to the emergency department. Research in this field is needed, since it would allow early identification and treatment of delirium.
Rivaroxaban Monotherapy in Patients with Pulmonary Embolism: Off-Label vs. Labeled Therapy
Authors: Di Micco Pierpaolo , Salazar Vladimir , Capitan Carmen , Dentali Francesco , Cuervo Covadonga , Reyes José , Porras Jose , Fidalgo Angeles , Grandone Elvira , Meseguer Manuel , Monreal Manuel ,   the RIETE Investigators
Published: July 2022

Background: The use of rivaroxaban in clinical practice often deviates from manufacturer prescribing information. No studies have demonstrated an association between this practice and improved outcomes. Methods: We used the RIETE registry to assess the clinical characteristics of patients with pulmonary embolism (PE) who received off-label rivaroxaban, and to compare their 3-month outcomes with those receiving the labeled therapy. The patients were classified into four subgroups: (1) labeled therapy; (2) delayed start; (3) low doses and (4) both conditions. Results: From May 2013 to May 2022, 2490 patients with PE received rivaroxaban: labeled therapy—1485 (58.6%); delayed start—808 (32.5%); low doses—143 (5.7%); both conditions—54 (2.2%). Patients with a delayed start were more likely to present with syncope, hypotension, raised troponin levels and more severe abnormalities on the echocardiogram than those on labeled therapy. Patients receiving low doses were most likely to have cancer, recent bleeding, anemia, thrombocytopenia or renal insufficiency. During the first 3 months, 3 patients developed PE recurrence, 4 had deep-vein thrombosis, 11 had major bleeding and 16 died. The rates of major bleeding (11 vs. 0; p < 0.001) or death (15 vs. 1; OR: 22.5; 95% CI: 2.97–170.5) were higher in patients receiving off-label rivaroxaban than in those on labeled therapy, with no differences in VTE recurrence (OR: 1.11; 95% CI: 0.25–6.57). Conclusions: In patients with severe PE, the start of rivaroxaban administration was often delayed. In those at increased risk for bleeding, it was often prescribed at low doses. Both subgroups had a worse outcome than those on labeled rivaroxaban.
Dihydroartemisinin Reduces Irradiation-Induced Mitophagy and Radioresistance in Lung Cancer A549 Cells via CIRBP Inhibition
Authors: Wu Shunlong , Li Zhaodong , Li Haiyu , Liao Kui
Published: July 2022

Radiotherapy is a major therapeutic strategy for lung cancer, and radiation resistance (radioresistance) is an important cause of residual and recurring cancer after treatment. However, the mechanism of radioresistance remains unclear. Mitochondrial autophagy (mitophagy), an important selective autophagy, plays an important role in maintaining cell homeostasis and affects the response to therapy. Recent studies have shown that dihydroartemisinin (DHA), a derivative of artemisinin, can increase the sensitivity to treatment in multiple types of cancer, including lung cancer. The purpose of this study was to elucidate the function and molecular mechanisms of DHA-regulating mitophagy and DHA-reducing radioresistance in lung cancer A549 cells. We first constructed the radioresistant lung cancer A549 cells model (A549R) through fractional radiation, then elucidated the function and mechanism of DHA-regulating mitophagy to reduce the radioresistance of lung cancer by genomic, proteomic, and bioinformatic methods. The results showed that fractional radiation can significantly induce radioresistance and mitophagy in A549 cells, DHA can reduce mitophagy and radioresistance, and the inhibition of mitophagy can reduce radioresistance. Protein chip assay and bioinformatics analysis showed the following: Cold-Inducible RNA Binding Protein (CIRBP) might be a potential target of DHA-regulating mitophagy; CIRBP is highly expressed in A549R cells; the knockdown of CIRBP increases the effect of DHA, reduces mitophagy and radioresistance, and inhibits the mitophagy-related PINK1/Parkin pathway. In conclusion, we believe that DHA reduces radiation-induced mitophagy and radioresistance of lung cancer A549 cells via CIRBP inhibition.
Bioactive Compounds in Garlic (Allium sativum) and Black Garlic as Antigout Agents, Using Computer Simulation
Authors: Lestari Ayu Rahmania , Batubara Irmanida , Wahyudi Setyanto Tri , Ilmiawati Auliya , Achmadi Suminar Setiati
Published: July 2022

Uric acid, which causes gout, is the end product of purine catabolism, synthesized by xanthine oxidase, guanine deaminase, adenine deaminase, purine nucleoside phosphorylase, and 5-nucleotidase II. Garlic contains bioactive compounds that have potential as antigout agents. Garlic fermentation to black garlic changes its components, which may affect its beneficial potential. This study aimed to select types of garlic (Indonesian garlic) and imported garlic, and to predict the interaction between their compounds and five target proteins through an in silico approach and a multivariate analysis, namely partial least squares-discriminant analysis (PLS-DA), to determine their different constituents. The target proteins were collected from open-access databases, and the compounds were identified using mass spectrometry data. The PLS-DA score plot succeeded in classifying the samples into three classes, with each class having a discriminatory compound. Based on the in silico studies, we predicted the best binding score of the five target proteins with seven important compounds: alliin, N-acetyl-S-allyl-L-cysteine, ajoene, pyridoxal, pyridoxamine, 4-guanidinobutyric acid, and D-glucosamine. These were mostly found in black garlic, with no different concentrations in the local and imported samples. Through this approach, we concluded that black garlic is a better candidate for antigout treatments, as several compounds were found to have good binding to the target proteins.
Seroprevalence of Hepatitis E Virus Antibodies (IgG) in the Community of Rawalpindi
Authors: Farooqi Muhammad Awais , Ahsan Ali , Yousuf Shazia , Shakoor Noman , Farooqi Hafiz Muhammad Umer
Published: July 2022

Knowledge regarding the prevalence of the hepatitis E virus (HEV) in the general population can indicate public health and personal hygiene practices in a community. HEV spreads through the fecal-oral route and contaminates drinking water through sewage. Moreover, poverty also contributes to its prevalence in developing countries, including Pakistan. A cross-sectional study was conducted on 650 blood samples taken from suspected patients of HEV in the Rawalpindi cantonment area (Pakistan) from April to November 2019 at the Department of Virology, Armed Forces Institute of Pathology (AFIP), Rawalpindi, Pakistan. Out of them, 444 (68.15%) were male and 206 (31.85%) were female; the detection of anti-HEV IgG antibodies was carried out using a commercial Anti-Hepatitis E virus antibody (IgG) ELISA Kit. The overall anti-HEV IgG prevalence percentages were 19.23% and 4.77% in males and females, respectively. Patients were categorized into eight groups with ages ranging between 1 and 90 years. HEV IgG seroprevalence was the highest in ages 31–40 (6.46%). The study concluded that males aged 40 or above were susceptible and infected with hepatitis E.
Tracing the Status of Silica Fume in Cementitious Materials Subjected to Deterioration Mechanisms with Raman Microscope
Authors: Yue Yanfei , Wang Jingjing , Bai Yun
Published: July 2022

The status and stability of the unreacted silica fume (SF) agglomerates existing in concrete structures subjected to various deterioration environments is largely unknown, but is a critical issue which could cause public concern. This work employed a Raman microscope, which combines the Raman spectroscopy with a light optical microscope, to characterize the phase assemblage in 6-month-old SF blended Portland cement (PC) pastes after 3-month exposure to simulated deterioration mechanisms (viz. carbonation, chloride attack, or sulfate attack), in order to illustrate the status of SF. Unhydrated SF phases, in terms of amorphous silica (Raman shift at about 350–540 cm−1), were identified in the SF blended paste samples after being exposed to carbonation and sulfate attack, indicating that there is a potential hazard to the living system, especially the structures undergoing long-term ‘interactions’ with a contiguous environment.
Formation of Gold Nanoparticle Self-Assembling Films in Various Polymer Matrices for SERS Substrates
Authors: Maleeva Ksenia A. , Kaliya Ilia E. , Tkach Anton P. , Babaev Anton A. , Baranov Michail A. , Berwick Kevin , Perova Tatiana S. , Baranov Alexander V. , Bogdanov Kirill V.
Published: July 2022

Surface-enhanced Raman spectroscopy (SERS) is regarded as a versatile tool for studying the composition and structure of matter. This work has studied the preparation of a SERS substrate based on a self-assembling plasmonic nanoparticle film (SPF) in a polymer matrix. Several synthesis parameters for the SPF are investigated, including the size of the particles making up the film and the concentration and type of the self-assembling agent. The result of testing systems with different characteristics is discussed using a model substance (pseudoisocyanin iodide). These models can be useful in the study of biology and chemistry. Research results contain the optimal parameters for SPF synthesis, maximizing the SERS signal. The optimal procedure for SPF assembly is determined and used for the synthesis of composite SPFs within different polymer matrices. SPF in a polymer matrix is necessary for the routine use of the SERS substrate for various types of analytes, including solid samples or those sensitive to contamination. Polystyrene, polyvinyl alcohol (PVA), and polyethylene are investigated to obtain a polymer matrix for SPF, and various methods of incorporating SPF into a polymer matrix are being explored. It is found that films with the best signal enhancement and reproducibility were obtained in polystyrene. The minimum detectable concentration for the SERS substrate obtained is equal to 10−10 M. We prepared a SERS substrate with an analytical enhancement factor of 2.7 × 104, allowing an increase in the detection sensitivity of analyte solutions of five orders of magnitude.
Mitigation of the Adverse Impact of Copper, Nickel, and Zinc on Soil Microorganisms and Enzymes by Mineral Sorbents
Authors: Wyszkowska Jadwiga , Borowik Agata , Zaborowska Magdalena , Kucharski Jan
Published: July 2022

Despite numerous studies on the influence of heavy metals on soil health, the search for effective, eco-friendly, and economically viable remediation substances is far from over. This encouraged us to carry out a study under strictly controlled conditions to test the effects of Cu2+, Ni2+, and Zn2+ added to soil in amounts of 150 mg·kg−1 d.m. of soil on the soil microbiome, on the activity of two oxidoreductases and five hydrolases, and on the growth and development of the sunflower Helianthus annunus L. The remediation substances were a molecular sieve, halloysite, sepiolite, expanded clay, zeolite, and biochar. It has been demonstrated that the most severe turbulences in the soil microbiome, its activity, and the growth of Helianthus annunus L. were caused by Ni2+, followed by Cu2+, and the mildest negative effect was produced by Zn2+. The adverse impact of heavy metals on the soil microbiome and its activity was alleviated by the applied sorbents. Their application also contributed to the increased biomass of plants, which is significant for the successful phytoextraction of these metals from soil. Irrespective of which property was analysed, sepiolite can be recommended for the remediation of soil polluted with Ni2+ and zeolite—for soil polluted with Cu2+ and Zn2+. Both sorbents mitigated to the highest degree disturbances caused by the tested metals in the soil environment.
A PCA–EEMD–CNN–Attention–GRU–Encoder–Decoder Accurate Prediction Model for Key Parameters of Seawater Quality in Zhanjiang Bay
Authors: Xie Zaimi , Li Zhenhua , Mo Chunmei , Wang Ji
Published: July 2022

In order to effectively solve the problem of low accuracy of seawater water quality prediction, an optimized water quality parameter prediction model is constructed in this paper. The model first screened the key factors of water quality data with the principal component analysis (PCA) algorithm, then realized the de-noising of the key factors of water quality data with an ensemble empirical mode decomposition (EEMD) algorithm, and the data were input into the two-dimensional convolutional neural network (2D-CNN) module to extract features, which were used for training and learning by attention, gated recurrent unit, and an encoder–decoder (attention–GRU–encoder–decoder, attention–GED) integrated module. The trained prediction model was used to predict the content of key parameters of water quality. In this paper, the water quality data of six typical online monitoring stations from 2017 to 2021 were used to verify the proposed model. The experimental results show that, based on short-term series prediction, the root mean square error (RMSE), mean absolute percentage error (MAPE), and decision coefficient (R2) were 0.246, 0.307, and 97.80%, respectively. Based on the long-term series prediction, RMSE, MAPE, and R2 were 0.878, 0.594, and 92.23%, respectively, which were all better than the prediction model based on an enhanced clustering algorithm and adam with a radial basis function neural network (ECA–Adam–RBFNN), a prediction model based on a softplus extreme learning machine method with partial least squares and particle swarm optimization (PSO–SELM–PLS), and a wavelet transform-depth Bi–S–SRU (Bi-directional Stacked Simple Recurrent Unit) prediction model. The PCA–EEMD–CNN–attention–GED prediction model not only has high prediction accuracy but can also provide a decision-making basis for the water quality control and management of aquaculture in the waters around Zhanjiang Bay.
Performance of 3D-Printed Bionic Conch-Like Composite Plate under Low-Velocity Impact
Authors: Wan Mincen , Hu Dayong , Pei Baoqing
Published: July 2022

Biological armors can provide an effective protection against predators. In this study, inspired by conch shell, beetle exoskeleton, and nacre, three different types of bionic composites plates were fabricated: Bio-S, Bio-B, and Bio-N, as well as an equivalent monolithic plate formed from the same stiff material designed and manufactured by additive manufacturing, respectively. Low velocity impact tests using drop tower were conducted to study their impact resistance. Experimental findings indicated that the Bio-S composite had superior impact resistance compared with the other bionic composites and the monolithic plate. Furthermore, the influence of the ply angle on the impact resistance of the Bio-S composite plate was investigated. The (0°/30°/0°/30°) arrangement was able to provide the highest impact resistance. Finally, the crack propagation mode in Bio-S composites plates was analyzed, enhancing our understanding of the underlying mechanisms during impact. Such findings may lead to the development of superior lightweight protective structures with improved anti-impact performance.