bluuebunny/crossref_metadata_2025 huggingface dataset:
Internet of Medical Things with a Blockchain-Assisted Smart Healthcare System Using Metaheuristics with a Deep Learning Model
Authors: Albakri Ashwag , Alqahtani Yahya Muhammed
Published: May 2023

The Internet of Medical Things (IoMT) is a network of healthcare devices such as wearables, diagnostic equipment, and implantable devices, which are linked to the internet and can communicate with one another. Blockchain (BC) technology can design a secure, decentralized system to store and share medical data in an IoMT-based intelligent healthcare system. Patient records were stored in a tamper-proof and decentralized way using BC, which provides high privacy and security for the patients. Furthermore, BC enables efficient and secure sharing of healthcare data between patients and health professionals, enhancing healthcare quality. Therefore, in this paper, we develop an IoMT with a blockchain-based smart healthcare system using encryption with an optimal deep learning (BSHS-EODL) model. The presented BSHS-EODL method allows BC-assisted secured image transmission and diagnoses models for the IoMT environment. The proposed method includes data classification, data collection, and image encryption. Initially, the IoMT devices enable data collection processes, and the gathered images are stored in BC for security. Then, image encryption is applied for data encryption, and its key generation method can be performed via the dingo optimization algorithm (DOA). Finally, the BSHS-EODL technique performs disease diagnosis comprising SqueezeNet, Bayesian optimization (BO) based parameter tuning, and voting extreme learning machine (VELM). A comprehensive set of simulation analyses on medical datasets highlights the betterment of the BSHS-EODL method over existing techniques with a maximum accuracy of 98.51%, whereas the existing methods such as DBN, YOLO-GC, ResNet, VGG-19, and CDNN models have lower accuracies of 94.15%, 94.24%, 96.19%, 91.19%, and 95.29% respectively.
Assessment of Rainwater Quality Regarding its Use in The Roztocze National Park (Poland)—Case Study
Authors: Grabowski Tadeusz , Jóźwiakowski Krzysztof , Bochniak Andrzej , Stachyra Przemysław , Radliński Bogusław
Published: May 2023

The aim of this study was to determine the quality of rainwater and the possibility of using it for various purposes in the Roztocze National Park (RNP), Poland. This study was carried out in 2021–2022. Samples of rainwater that drained from the roofs of farm buildings in the RNP were tested for their organoleptic, physicochemical and microbiological qualities. The organoleptic tests were run to evaluate the water for a foreign odour and the threshold odour number. The physical and chemical tests included turbidity; colour; pH; conductivity; concentrations of ammonium ions, nitrates, nitrites, manganese, iron and chlorides; and general hardness. The microbiological tests included total microbial counts at 36 °C and 22 °C, coliform bacteria, Escherichia coli, intestinal enterococci and Pseudomonas aeruginosa. The rainwater quality results were compared with the quality parameters of surface water collected from the River Świerszcz, as well as with the Polish drinking water standards. The findings indicated that rainwater collected in the RNP had good organoleptic, physicochemical and microbiological properties, which, in some cases, complied with the standards for potable water. Exceedances of the permissible limits, mainly for ammonium ions and microbiological indicators, were periodically observed in the tested rainwater. This was probably due to contamination of roof surfaces with bird droppings. However, these exceedances did not exclude the use of the rainwater for economic purposes, e.g., flushing toilets, washing vehicles or watering plants, which may significantly reduce the abstraction of high-quality groundwater. The rainwater that is planned to be used as drinking water for the Polish konik horses living in the park will have to be pre-treated via filtration and disinfection processes (e.g., with a UV lamp).
Changes in Physical and Chemical Parameters of Beetroot and Carrot Juices Obtained by Lactic Fermentation
Authors: Janiszewska-Turak Emilia , Pobiega Katarzyna , Rybak Katarzyna , Synowiec Alicja , Woźniak Łukasz , Trych Urszula , Gniewosz Małgorzata , Witrowa-Rajchert Dorota
Published: May 2023

Fermented foods have gained popularity recently, especially lactofermented vegetable juice products that provide a rich source of nutrients. This study analyzed the properties of fermented beetroot, carrot and beetroot-carrot juices fermented with bacterial strains Lactiplantibacillus plantarum or Levilactobacillus brevis. Density, extract, dry matter content, pH, total acidity, pigments (betalain or carotenoids), color and LAB count were measured. The results showed that the LAB strains used were capable of fermenting the analyzed juices. It was proven that Lactiplantibacillus plantarum was the better strain for fermentation of vegetable juice. This might have been influenced by the fermentation temperature and the duration time. The highest number of lactic acid bacteria was observed for carrot juice fermented with L. brevis on the 4th day (9.41 log CFU/mL), while for L. plantarum the highest numbers were observed on the 5th–6th day (8.9–9.1 log CFU/mL). For beetroot and its mixture with carrot juices, higher results were observed on the 5th day for L. plantarum (9.0 and 8.3 log CFU/mL, respectively). In most variants of this process, fermentation can be completed after 4–5 days. During the fermentation of carrot and mixed juice slow degradation of carotenoids was observed, while in mixed juice an increase of red pigment (betanin) was observed. In beetroot juices huge degradation was observed for fermentation with L. brevis, while the second bacteria kept the same level of red pigment almost until the last day, while yellow decreased from the 3rd day. It can be concluded that the mixed juice was most stable according to the bacteria count and the pigment content. In our opinion, L. plantarum was better for the fermentation of juices than L. brevis. However, in future tests the time of fermentation can be shortened to 5 days and other LAB strains can be tested.
DMS-YOLOv5: A Decoupled Multi-Scale YOLOv5 Method for Small Object Detection
Authors: Gao Tianyu , Wushouer Mairidan , Tuerhong Gulanbaier
Published: May 2023

Small objects detection is a challenging task in computer vision due to the limited semantic information that can be extracted and the susceptibility to background interference. In this paper, we propose a decoupled multi-scale small object detection algorithm named DMS-YOLOv5. The algorithm incorporates a receptive field module into the feature extraction network for better focus on low-resolution small objects. The coordinate attention mechanism, which combines spatial and channel attention information, is introduced to reduce interference from background information and enhance the network’s attention to object information. A detection layer tailored to small-sized objects is added to compensate for the loss of small object information in multiple downsampling operations, greatly improving the detection capability of small objects. Next, The decoupled network is introduced into the detection head network for branch processing of classification and bounding box regression tasks. Finally, the bounding box loss function is improved to alleviate missed detection problems caused by the concentration of small objects and mutual occlusion between objects. The improved method achieved a mean average precision improvement of 12.1% on VisDrone2019-DET dataset compared to the original method. In comparison experiments with similar methods, our proposed method also demonstrated good performance, validating its effectiveness.
Optimal Statistical Analyses of Bell Experiments
Authors: Gill Richard D.
Published: May 2023

We show how both smaller and more reliable p-values can be computed in Bell-type experiments by using statistical deviations from no-signalling equalities to reduce statistical noise in the estimation of Bell’s S or Eberhard’s J. Further improvement was obtained by using the Wilks likelihood ratio test based on the four tetranomially distributed vectors of counts of the four different outcome combinations, one 4-vector for each of the four setting combinations. The methodology was illustrated by application to the loophole-free Bell experiments of 2015 and 2016 performed in Delft and Munich, at NIST, and in Vienna, respectively, and also to the earlier (1998) Innsbruck experiment of Weihs et al. and the recent (2022) Munich experiment of Zhang et al., which investigates the use of a loophole-free Bell experiment as part of a protocol for device-independent quantum key distribution (DIQKD).
Dealing with Evaluative Expressions and Hate Speech Metaphors with Fuzzy Property Grammar Systems
Authors: Torrens-Urrutia Adrià , Jiménez-López Maria Dolores , Campillo-Muñoz Susana
Published: May 2023

We introduce a Fuzzy Property Grammar System (FPGS), a formalism that integrates a Fuzzy Property Grammar into a linguistic grammar system to formally characterize metaphorical evaluative expressions. The main scope of this paper is to present the formalism of FPGS and to show how it might provide a formal characterization of hate speech linguistic evaluative expressions with metaphors (as fuzzy concepts), together with evaluating their degree of linguistic violence. Linguistic metaphors are full of semantic coercions. It is necessary to formally characterize the context of the communication to acknowledge the extralinguistic constraints of the pragmatic domain, which establishes whether an utterance is violent. To show the applicability of our formalism, we present a proof of concept. By compiling and tagging a 3000-tweet corpus, we have extracted a lexicon of hate speech metaphors. Furthermore, we show how FPGS architecture can deal with different types of hate speech and can identify implicit violent figurative evaluative expressions by context and type. Although we are still in the experimental phase of our project and cannot present conclusive results at the computational level, the proof-of-concept results show that our formalism can achieve the desired outcome.
Controlling the Molar Ratios of Cation to Anion of Precursors for High Performance Capacitive Properties of MnO2 Hybridized Carbon-Based Materials Electrode
Authors: Yang Wein-Duo , Chou Yi-Rong , Kuo Cheng-Ching , Kang Yu-Min
Published: May 2023

Controlling the cation to anion (Mn2+/MnO4−) molar ratios of the precursors was used to obtain a highly performance capacitive properties of nanostructural MnO2 hybridized carbon-based materials on nickel foam (NF) through successive ionic layer adsorption and reaction technology. SEM, XRD, BET, and XPS analyses are utilized to investigate the influence of cation/anion molar ratios of precursors on the as-obtained MnO2 electrode materials. At a lower molar ratio of cation/anion of 1, the prepared manganese oxide deposited on the NF with obvious δ-MnO2 phase. The average pore size distribution of BET analysis of the as-obtained δ-MnO2 is about 4.6 nm, the specific surface area is 155.7 m2 g−1, exhibiting a mesoporous structure. However, when the molar ratio of cation/anion is higher than 5, the deposited film produced by the reaction exhibits a γ-MnO2 crystal phase. The capacitance of δ-MnO2/NF electrode is 280 F g−1 at 1 A g−1 in a 1 M Na2SO4 aqueous electrolyte solution. In addition, reduced graphene oxide (rGO) mixed with multi-wall carbon nanotube (MWCNT) was added to synthesize γ-MnO2/rGO-MWCNT/NF electrode, which has a high capacitance of 377.4 F g−1 under the charge/discharge current density at 1 A g−1.
Reduction in mRNA Expression of the Neutrophil Chemoattract Factor CXCL1 in Pseudomonas aeruginosa Treated Barth Syndrome B Lymphoblasts
Authors: Zegallai Hana M. , Duan Kangmin , Hatch Grant M.
Published: May 2023

Barth Syndrome (BTHS) is a rare X-linked genetic disease caused by a mutation in the TAFAZZIN gene, which codes for the protein tafazzin involved in cardiolipin remodeling. Approximately 70% of patients with BTHS exhibit severe infections due to neutropenia. However, neutrophils from BTHS patients have been shown to exhibit normal phagocytosis and killing activity. B lymphocytes play a crucial role in the regulation of the immune system and, when activated, secrete cytokines known to attract neutrophils to sites of infection. We examined the expression of chemokine (C-X-C motif) ligand 1 (CXCL1), a known chemotactic for neutrophils, in Epstein–Barr virus transformed control and BTHS B lymphoblasts. Age-matched control and BTHS B lymphoblasts were incubated with Pseudomonas aeruginosa for 24 h and then cell viability, CD27+, CD24+, CD38+, CD138+ and PD1+ surface marker expression and CXCL1 mRNA expression determined. Cell viability was maintained in lymphoblasts incubated in a ratio of 50:1 bacteria:B cells. Surface marker expression was unaltered between control and BTHS B lymphoblasts. In contrast, CXCL1 mRNA expression was reduced approximately 70% (p < 0.05) in untreated BTHS B lymphoblasts compared to control and approximately 90% (p < 0.05) in bacterial treated BTHS B lymphoblasts compared to the control. Thus, naïve and bacterial-activated BTHS B lymphoblasts exhibit reduced mRNA expression of the neutrophil chemoattractant factor CXCL1. We suggest that impaired bacterial activation of B cells in some BTHS patients could influence neutrophil function via impairing neutrophil recruitment to sites of infection and this could potentially contribute to these infections.
Gonad Ontogeny and Sex Differentiation in a Poeciliid, Gambusia holbrooki: Transition from a Bi- to a Mono-Lobed Organ
Authors: Razmi Komeil , Tran Ngoc Kim , Patil Jawahar G.
Published: May 2023

Despite their uniqueness, the ontogeny and differentiation of the single-lobed gonads in the poeciliids are very poorly understood. To address this, we employed both cellular and molecular approaches to systematically map the development of the testes and ovary in Gambusia holbrooki from pre-parturition to adulthood, encompassing well over 19 developmental stages. The results show that putative gonads form prior to the completion of somitogenesis in this species, a comparatively early occurrence among teleosts. Remarkably, the species recapitulates the typical bi-lobed origin of the gonads during early development that later undergoes steric metamorphosis to form a single-lobed organ. Thereafter, the germ cells undergo mitotic proliferation in a sex-dependent manner before the acquisition of the sexual phenotype. The differentiation of the ovary preceded that of the testes, which occurred before parturition, where the genetic females developed meiotic primary oocytes stage I, indicating ovarian differentiation. However, genetic males showed gonial stem cells in nests with slow mitotic proliferation at the same developmental stage. Indeed, the first signs of male differentiation were obvious only post-parturition. The expression pattern of the gonadosoma markers foxl2, cyp19a1a, amh and dmrt1 in pre- and post-natal developmental stages were consistent with morphological changes in early gonad; they were activated during embryogenesis, followed by the onset of gonad formation, and a sex-dimorphic expression pattern concurrent with sex differentiation of the ovary (foxl2, cyp19a1a) and testes (amh and dmrt1). In conclusion, this study documents for the first time the underlying events of gonad formation in G. holbrooki and shows that this occurs relatively earlier than those previously described for ovi- and viviparous fish species, which may contribute to its reproductive and invasive prowess.
Non-Psychoactive Cannabinoid Modulation of Nociception and Inflammation Associated with a Rat Model of Pulpitis
Authors: Laks Elana Y. , Li Hongbo , Ward Sara Jane
Published: May 2023

Despite advancements in dental pain management, one of the most common reasons for emergency dental care is orofacial pain. Our study aimed to determine the effects of non-psychoactive Cannabis constituents in the treatment of dental pain and related inflammation. We tested the therapeutic potential of two non-psychoactive Cannabis constituents, cannabidiol (CBD) and β-caryophyllene (β-CP), in a rodent model of orofacial pain associated with pulp exposure. Sham or left mandibular molar pulp exposures were performed on Sprague Dawley rats treated with either vehicle, the phytocannabinoid CBD (5 mg/kg i.p.) or the sesquiterpene β-CP (30 mg/kg i.p.) administered 1 h pre-exposure and on days 1, 3, 7, and 10 post-exposure. Orofacial mechanical allodynia was evaluated at baseline and post-pulp exposure. Trigeminal ganglia were harvested for histological evaluation at day 15. Pulp exposure was associated with significant orofacial sensitivity and neuroinflammation in the ipsilateral orofacial region and trigeminal ganglion. β-CP but not CBD produced a significant reduction in orofacial sensitivity. β-CP also significantly reduced the expression of the inflammatory markers AIF and CCL2, while CBD only decreased AIF expression. These data represent the first preclinical evidence that non-psychoactive cannabinoid-based pharmacotherapy may provide a therapeutic benefit for the treatment of orofacial pain associated with pulp exposure.
SARS-CoV-2 Antibody Responses in Pediatric Patients: A Bibliometric Analysis
Authors: Maniu Ionela , Maniu George Constantin , Antonescu Elisabeta , Duica Lavinia , Grigore Nicolae , Totan Maria
Published: May 2023

The characteristics, dynamics and mechanisms/determinants of the immune response to SARS-CoV-2 infection are not fully understood. We performed a bibliometric review of studies that have assessed SARS-CoV-2 antibody responses in the pediatric population using Web of Science online databases, VOSviewer and Bibliometrix tools. The analysis was conducted on 84 publications, from 310 institutions located in 29 countries and published in 57 journals. The results showed the collaboration of scientists and organizations, international research interactions and summarized the findings on (i) the measured titers of antibodies (total antibody and/or individual antibody classes IgG, IgM, IgA) against different antigens (C-terminal region of N (N CT), full-length N protein (N FL), RBD, RBD Alpha, RBD Beta, RBD Gamma, RBD Delta, spike (S), S1, S2) in the case of different clinical forms of the disease; and (ii) the correlations between SARS-CoV-2 antibodies and cytokines, chemokines, neutrophils, C-reactive protein, ferritin, and the erythrocyte sedimentation rate. The presented study offers insights regarding research directions to be explored in the studied field and may provide a starting point for future research.
Stromal Vascular Fraction in Osteoarthritis of the Knee
Authors: Jeyaraman Madhan , Maffulli Nicola , Gupta Ashim
Published: May 2023

In the United States, osteoarthritis (OA) affects 30 million people among the population and poses a major disability and financial burden that impact functional quality of life among the affected individuals [...]
Cerebrospinal Fluid–Basic Concepts Review
Authors: Czarniak Natalia , Kamińska Joanna , Matowicka-Karna Joanna , Koper-Lenkiewicz Olga
Published: May 2023

Cerebrospinal fluid plays a crucial role in protecting the central nervous system (CNS) by providing mechanical support, acting as a shock absorber, and transporting nutrients and waste products. It is produced in the ventricles of the brain and circulates through the brain and spinal cord in a continuous flow. In the current review, we presented basic concepts related to cerebrospinal fluid history, cerebrospinal fluid production, circulation, and its main components, the role of the blood–brain barrier and the blood–cerebrospinal fluid barrier in the maintenance of cerebrospinal fluid homeostasis, and the utility of Albumin Quotient (QAlb) evaluation in the diagnosis of CNS diseases. We also discussed the collection of cerebrospinal fluid (type, number of tubes, and volume), time of transport to the laboratory, and storage conditions. Finally, we briefly presented the role of cerebrospinal fluid examination in CNS disease diagnosis of various etiologies and highlighted that research on identifying cerebrospinal fluid biomarkers indicating disease presence or severity, evaluating treatment effectiveness, and enabling understanding of pathogenesis and disease mechanisms is of great importance. Thus, in our opinion, research on cerebrospinal fluid is still necessary for both the improvement of CNS disease management and the discovery of new treatment options.
Multi-Locomotion Design and Implementation of Transverse Ledge Brachiation Robot Inspired by Sport Climbing
Authors: Lin Chi-Ying , Lee Jhe-Ming
Published: May 2023

Brachiation robots mimic the locomotion of bio-primates, including continuous brachiation and ricochetal brachiation. The hand-eye coordination involved in ricochetal brachiation is complex. Few studies have integrated both continuous and ricochetal brachiation within the same robot. This study seeks to fill this gap. The proposed design mimics the transverse movements of sports climbers holding onto horizontal wall ledges. We analyzed the cause-and-effect relationship among the phases of a single locomotion cycle. This led us to apply a parallel four-link posture constraint in model-based simulation. To facilitate smooth coordination and efficient energy accumulation, we derived the required phase switching conditions as well as joint motion trajectories. Based on a two-hand-release design, we propose a new style of transverse ricochetal brachiation. This design better exploits inertial energy storage for enhanced moving distance. Experiments demonstrate the effectiveness of the proposed design. A simple evaluation method based on the final robot posture from the previous locomotion cycle is applied to predict the success of subsequent locomotion cycles. This evaluation method serves as a valuable reference for future research.
The Effects of Repeated Attachment Security Priming on Social Anxiety and Attention Bias: A Randomized Controlled Trial
Authors: Zhang Shuang , Tao Yanqiang , Chen Yunxiang , Zhang Peng , Liu Xiangping
Published: May 2023

Background: Although the clinical utility of attachment security priming has been suggested in recent years, the effect of attachment security priming on social anxiety and its core symptoms (i.e., attention bias) remains unspecified. Therefore, the present study explored the potential effectiveness of repeated attachment security priming in alleviating social anxiety and attention bias among Chinese college students. Methods: Fifty-six college students with high social anxiety were randomly assigned to the attachment security priming group (n = 30) or control group (n = 26). The priming group completed seven attachment security priming sessions over 2 weeks (every 2 days), and the control group was assigned to a waitlist for 2 weeks. Results: The results revealed that individuals in the priming group reported less social anxiety after 2 weeks of security attachment priming, and those in the control group did not change significantly. The results also showed that there was no significant change in the attention bias of individuals with social anxiety before and after the intervention. Conclusions: Our findings indicate that attachment security priming is a promising alternative intervention option for social anxiety. The potential clinical implications of security attachment priming are discussed.
Lower Emotional Exhaustion among Employees Is Associated with Intentional Incorporation of Animals into Residential Care Settings
Authors: Tumlin Kimberly I. , Riley Elizabeth N. , Vsevolozhskaya Olga , Cull Michael
Published: May 2023

Secondary effects of animal-integrated programming on residential care center (RCC) staff and organizational culture are not well understood. We explored emotional exhaustion among RCC employees both in facilities that incorporated animals and those that did not incorporate animals into the therapeutic environment. We conducted a survey throughout a large midwestern RCC system in the United States to determine relationships between organizational culture, emotional exhaustion, and the intentionality by which animals were incorporated into programming. Data were analyzed by examining associations between variables of interest using chi-square or t-tests, and linear mixed-effects modeling was used to identify potential confounding effects due to differences in children served within RCCs. Staff from RCCs that used animals intentionally reported lower emotional exhaustion (p = 0.006), and higher average workplace safety (p = 0.024) and psychological safety (p < 0.001). Integrating animals into RCC programming is associated with elements of a strong organizational culture. It is possible that animal-integrated programming has a positive impact on the facility culture and workforce, and/or that RCCs with strong pre-existing cultures are more likely to use animal-integrated programming.
Study on Camping Behavior Patterns for Thermal Comfort at Riverside Parks
Authors: Wu Dainan , Liu Lin , Li Li
Published: May 2023

People-oriented landscape experiences have become the focus of park design with the increasing demand of outdoor activities from urban residents, whereas thermal-comfort-guided landscape design has attracted more attention in academic circles. Based on the investigation of the microclimate of typical subregions in riverside parks, this paper uses AI recognition to analyze the spatiotemporal distribution of camping crowds, and considers the correlation between landscape morphological parameters, microclimates, and crowd behavior. Finally, we built a model to raise the number of landscape optimization strategies for landscape design. The results show that landscape morphological parameters, such as tree height, crown canopy, and sky visibility factor (SVF), can significantly affect the air temperature (Ta), relative humidity (RH), and physiological temperature (PET) in an environment, while hardly affecting wind speed (WS). For microclimate parameters, Ta has a moderate correlation with camping behavior, with a correlation coefficient of −0.145 and a p-value of 0.040, while the corresponding correlation with PET was non-significant with a p-value of 0.622. The temporal distribution of the number of campers per day show a linear upward trend with a reasonable goodness-of-fit, with an adjusted R2 above 0.789 for all subregions. The model based on landscape morphological parameters has a good fit, with coefficients of tree height and crown canopy of −0.195 and 1.316, respectively. This study provides theoretical support and design suggestions for the design of riverside parks based on crowd behavior patterns.
Joint Ventures in the South African Construction Industry: Factors Militating against Success
Authors: Seiso Modisaotsile Patrick , Ogunbayo Babatunde Fatai , Aigbavboa Clinton Ohis
Published: May 2023

Joint construction ventures are a global business partnership approach to accomplishing jointly executed construction objectives. The success of joint ventures is not achieved without militating factors and complexity affecting the process. This study assessed the militating factors against joint venture success in the construction industry in South Africa. A quantitative research approach was adopted, using a purposive sampling technique to select participants for this study. In total, 190 copies of the questionnaire were sent out to construction stakeholders in Gauteng province, South Africa, and 185 copies of the questionnaire were retrieved. Data analysis was conducted in three stages: data reliability and validity, descriptive statistics, and exploratory factor analysis. The exploratory factor analysis (EFA) returned seven factors that provided a relevant understanding of the militating factors against joint venture success: differences in partners’ work values, ineffective regulatory frameworks, undefined goals, clashes between partners’ cultural values, economic viability, operational constraints, and conflicts of interest. This study recommends that joint venture formulation requires due diligence from partners to understand policy, organisational culture, sharing ratios, and economic viability to avoid unnecessary conflicts of interest and operational constraints.
Establishing the Relationship between Occupants’ Thermal Behavior and Energy Consumption during Showering
Authors: Zhang Dadi , Mui Kwok-Wai , Wong Ling-Tim
Published: May 2023

Despite an increased awareness about energy conservation in the past decade, the energy consumed for water heating has increased by 7% from 2008 (17%) to 2018 (24%) in Hong Kong. A literature review on existing energy-saving technologies during showering showed that occupants’ behavior significantly impacted energy consumption. However, the exact relationship between them was not yet fully understood. Therefore, this study developed a mathematical energy consumption model to investigate the relationship between occupants’ behavior and energy consumption during showering. This relationship identified an effective energy-saving strategy in the shower without scarifying occupants’ thermal comfort. The main variables that influence energy consumption and thermal comfort in bathrooms namely air temperature, water temperature, ventilation rate, and water flow rate, were considered. It was found that among them, water flow rate and ventilation rate are the most and least influential variables, respectively, in energy saving. Therefore, the ventilation rate was suggested to be at least 0.03 kg·s−1, and the water flow rate was meant to be lower than 0.15 kg·s−1 (based on related requirements). These findings could help residential occupants and facility managers determine the optimal showering settings for thermal comfort, energy consumption, and environmental effects.
Research on Dynamic Pile-Driving Formula Parameters and Driving Feasibility of Extra-Long PHC Pipe Piles
Authors: Liu Xiaomin , Xiao Yonggang , Zhou Junlong , Ge Longbo , Song Ziwen
Published: May 2023

Prestressed high-strength concrete (PHC) pipe pile has the advantages of high single pile bearing capacity, a wide range of applications, good driving resistance, fast construction speed, etc. It has been widely used in high-rise buildings, bridges, ports, and other industries. The application of extra-long PHC pipe piles with a length of more than 50 m is increasing. However, there are few studies on the drivability and hammering criteria of extra-long PHC piles. To analyze the drivability of extra-long piles and predict their bearing capacity, in this paper, high-strain dynamic tests were carried out on 14 test sections with the pile foundation of Temburong Bridge in Brunei as the research background. The hammer stop control criteria calculated according to the Hiley formula would lead to excessive hammering. Three types of damage occurred during construction: pile shaft breakage, weld tearing, and pile head breakage. The weight and drop height of the piling hammer selected for this project were appropriate, and the extra-long test piles can be hammered to the design depth. The values of Cp (Compression of the pile) and n (the efficiency of the blow) were fitted based on the dynamic test data, which provided a more accurate reference for the selection of subsequent piling parameters of the project. It provides a more accurate calculation method for predicting the bearing capacity of extra-long PHC piles and provides control criteria for pile stopping and a scientific basis for their design and construction.
Comparative Response Spectrum Analysis on 15- and 50-Story Reinforced Concrete Buildings Having Shear Walls with and without Openings as per EN1998-1 Seismic Code
Authors: Abate Mistreselasie , Evangelista Ana Catarina Jorge , Tam Vivian W. Y.
Published: May 2023

Medium-rise reinforced concrete (RC) framed apartment complexes with stories ranging from 15 to 50 are becoming more common in Ethiopia’s main cities. In these RC-framed structures, shear walls are included for lateral load resistance. As apertures are frequently provided in shear walls, it is critical to evaluate their influence on story drift, stiffness, shear and moments, and stress within the shear walls. A 3D study with five different cases was carried out with ETABS version 19.00 software to investigate the influence of apertures in a building’s shear wall. This study looks at the effects of changing the size and location of these apertures. Based on this analysis, extensive data were acquired, and useful conclusions were formed that will be useful to practicing engineers. The seismic parameter utilized for the response spectrum study was Building Code of Ethiopia ES8-15, which conforms to Eurocode 8-2004 seismic code guidelines (based on EN1998-1) with target response spectrum type-I. The following parameters were used: ground acceleration, ag/g = 0.1, spectrum type = I, ground type = B, soil factor, S = 1.35, spectrum period, Tb, = 0.05 s, spectrum period, Tc = 0.25 s, spectrum period, Td = 1.2 s, lower bound factor, beta = 0.2, behavior factor = 1, and damping ratio = 5%. The outcomes are compared using various parameters such as displacement, story drift, story stiffness, story shear, and story moment both with and without shear wall opening cases. This study will give tremendous insight into the effect of shear wall openings on the performance of the structure. The analysis in this work was carried out on a linear model, which may not represent the complete local response of the structure; thus, future researchers should perform nonlinear analysis based on a performance-based design. It was concluded from this investigation that incorporating shear walls considerably enhanced the performance of the building over framed structures. Shear wall openings in a structure have a significant influence on the building’s performance. Due to their significant resistance to earthquake forces, shear wall structures are highly recommended for seismic hazard zones.
Tackling of Immunorefractory Tumors by Targeting Alternative Immune Checkpoints
Authors: Dulal Dharmindra , Boring Andrew , Terrero David , Johnson Tiffany , Tiwari Amit K. , Raman Dayanidhi
Published: May 2023

Physiologically, well known or traditional immune checkpoints (ICs), such as CTLA-4 and PD-1, are in place to promote tolerance to self-antigens and prevent generation of autoimmunity. In cancer, the ICs are effectively engaged by the tumor cells or stromal ells from the tumor microenvironment through expression of cognate ligands for the ICs present on the cell surface of CD8+ T lymphocytes. The ligation of ICs on CD8+ T lymphocytes triggers inhibitory signaling pathways, leading to quiescence or an exhaustion of CD8+ T lymphocytes. This results in failure of immunotherapy. To overcome this, several FDA-approved therapeutic antibodies are available, but the clinical outcome is quite variable due to the resistance encountered through upregulated expression of alternate ICs such as VISTA, LAG-3, TIGIT and TIM-3. This review focuses on the roles played by the traditional as well as alternate ICs and the contribution of associated signaling pathways in generating such resistance to immunotherapy. Combinatorial targeting of traditional and alternate ICs might be beneficial for immune-refractory tumors.
Gastric Cancer Risk in Association with Underweight, Overweight, and Obesity: A Systematic Review and Meta-Analysis
Authors: Azizi Narges , Zangiabadian Moein , Seifi Golnoosh , Davari Afshan , Yekekhani Elham , Safavi-Naini Seyed Amir Ahmad , Berger Nathan A. , Nasiri Mohammad Javad , Sohrabi Mohammad-Reza
Published: May 2023

This study aimed to investigate the risk of gastric cancer (GC) in abnormal body mass index (BMI) groups. A systematic search was carried out on Embase, PubMed/Medline, and Scopus from January 2000 to January 2023. The pooled risk ratio (RR) with a 95% confidence interval (CI) was assessed using a random-effect model. Thirteen studies with total of 14,020,031 participants were included in this systematic review. The pooled RR of GC was 1.124 (95% CI, 0.968–1.304, I2: 89.08%) in underweight class, 1.155 (95% CI, 1.051–1.270, I2: 95.18%) in overweight class, and in 1.218 (95% CI, 1.070–1.386, I2: 97.65%) obesity class. There is no difference between cardia and non-cardia gastric cancer, while non-Asian race and female gender have higher risk of cancer, as Meta-regression of obesity and overweight classes showed. These findings suggest that there is a positive association between excess body weight and the risk of GC, with a higher impact in women than men and in non-Asian than Asian populations. Since abnormal weight is tied to various diseases, including GC, healthcare experts, and policymakers should continue interventions aiming to achieve a normal BMI range.
Cytotoxic Activity, Anti-Migration and In Silico Study of Black Ginger (Kaempferia parviflora) Extract against Breast Cancer Cell
Authors: Hairunisa Indah , Bakar Mohd Fadzelly Abu , Da’i Muhammad , Bakar Fazleen Izzany Abu , Syamsul Eka Siswanto
Published: May 2023

Metastatic breast cancer remains the leading cause of death in women worldwide. This condition necessitates extensive research to find an effective treatment, one of which is the natural medicine approach. Kaempferia parviflora (KP) is a plant believed to possess anticancer properties. Therefore, this study aims to determine KP’s bioactive compound, cytotoxic, and anti-migration activity in the highly metastatic breast cancer cell line model 4T1, also in the breast cancer cell model MCF-7 and noncancerous cell line NIH-3T3. Maceration with ethanol (EEKP) and infusion with distilled water (EWKP) was used for extraction. The MTT assay was used to test for cytotoxicity, and the scratch wound healing assay was used to test for the inhibition of migration. Phytochemical profiling of EEKP was performed using UHPLC-MS, and the results were studied for in silico molecular docking. Result showed that EEKP had a better cytotoxic activity than EWKP with an IC50 value of 128.33 µg/mL (24 h) and 115.09 µg/mL (48 h) on 4T1 cell line, and 138.43 µg/mL (24 h) and 124.81 µg/mL (48 h) on MCF-7 cell line. Meanwhile, no cytotoxic activity was observed at concentrations ranging from 3–250 µg/mL in NIH-3T3. EEKP also showed anti-migration activity in a concentration of 65 µg/mL. Mass Spectrophotometer (MS) structures from EEKP are 5-Hydroxy-7,4′-dimethoxyflavanone (HDMF), 5-Hydro-7,8,2′-trimethoxyflavanone (HTMF), Retusine, and Denbinobin. The in silico docking was investigated for receptors Bcl-2, Bcl-XL, ERK2, and FAK, as well as their activities. In silico result indicates that HTMF and denbinobin are bioactive compounds responsible for EEKP’s cytotoxic and anti-migration activity. These two compounds and standardized plant extract can be further studied as potential breast cancer treatment candidates.
Intracranial Germinoma—Association between Delayed Diagnosis, Altered Clinical Manifestations, and Prognosis
Authors: Jabłońska Iwona , Goławski Marcin , Nowicka Elżbieta , Drosik-Rutowicz Katarzyna , Trybus Anna , Tarnawski Rafał , Miszczyk Marcin
Published: May 2023

Background: Intracranial germinoma is a rare malignant neoplasm of the central nervous system (CNS) that occurs in children and young adults. The aim of our study was to assess the initial manifestation of the disease, and to find differences in outcomes dependent on time of diagnosis. Methods: The study group consisted of 35 consecutive patients (adults and children) who were treated for intracranial germinoma with radiotherapy at a tertiary centre, and their data were retrospectively collected. We evaluated time from the first symptoms to diagnosis and divided patients into early and delayed diagnosis groups. Delayed diagnosis has been defined as the time from initial presentation to final diagnosis longer than six months. Results: A total of 17 (48.6%) of the patients had delayed diagnoses. Patient survival data spanned a median of six (interquartile range 3–12) years. At the time of the diagnosis, patients presented exclusively neurological symptoms in 16 (45.7%) cases, exclusively endocrinological symptoms in five (14.3%) cases, and mixed symptoms in the remaining cases (n = 14; 40.0%). Patients with neurological symptoms had shorter time (p < 0.001) from first symptoms to the final diagnosis (5.91 months) than in patients without them (19.44 months). The delayed diagnosis group presented significantly smaller tumour size (mean maximal dimension 2.35 cm) compared to early diagnosis group (3.1 cm). The 5-year and 10-year survival rates of our patients were 94.3% and 83.4%, respectively. Patients with a delayed diagnosis (n = 17) had a significantly worse (p = 0.02) 10-year OS (63%) compared to the early diagnosis group (n = 18; OS = 100%). Importantly, in five patients (14.29%), initial manifestation occurred before radiological signs of the disease. Conclusion: Our study stresses the need for timely diagnosis in intracranial germinoma, as a delay has a significant impact on the prognosis. In particular, if the tumour is small or causes exclusively endocrinological symptoms, the diagnosis may be difficult and delayed.
Novel Control System Strategy for the Catalytic Oxidation of VOCs with Heat Recovery
Authors: Miranda Angel Federico , Rodríguez María Laura , Serra Federico Martin , Borio Daniel Oscar
Published: May 2023

A theoretical study of the dynamic closed-loop behaviour of a reactor/feed-effluent heat exchanger (FEHE)/furnace system for the catalytic combustion of volatile organic compounds (VOCs) is presented. A 1D pseudohomogeneous plug-flow model is proposed to simulate the non-steady-state operation of the monolith reactor and the FEHE, while the furnace behaviour is described by means of a heterogeneous model of lumped parameters. Positive energy feedback is a source of instability that leads to strong thermal oscillations (limit cycles) and may cause damage to the equipment and sintering of the catalyst. The design of a robust and flexible control system and an efficient control strategy are, therefore, required to ensure safe and stable operation. The response of the system under three different control strategies to the most frequent disturbance variables—the feed flowrate (FV0) and feed concentration of VOCs (C0Et)—was evaluated. One of the control strategies consisted of a single-loop feedback system with servomechanism changes in the reactor inlet temperature (T0) that manipulated the bypass valve and, sequentially, the natural gas flowrate in the furnace (FNG). This approach made it possible to meet the control objective (reducing VOCs) without losing controllability and while minimizing the use of external fuel.
Evaluation of Heterogeneous Catalytic Ozonation Process for the Removal of Micropollutants from Water/Wastewater: Application of a Novel Pilot-Scale Continuous Flow System
Authors: Kaprara Efthimia , Belesakos Charalampos , Kollis Konstantinos , Psaltou Savvina , Zouboulis Anastasios , Mitrakas Manassis
Published: May 2023

The present study evaluates the removal of micropollutants from water/wastewater contaminated sources through the application of a heterogeneous catalytic ozonation process, using a pilot-scale continuous operation unit, composed of a membrane module for the diffusion and effective dilution of ozone into the liquid phase to be treated and a plug flow reactor/continuous stirred tank reactor (PFR/CSTR) contact reactor system in series, where the catalyst is recirculated in dispersion mode. The solid materials tested as catalysts are natural and calcined zeolite, Bayoxide and alumina, whereas the examined micropollutants, used in this case as probe compounds, are p-chlorobenzoic acid (p-CBA), atrazine, benzotriazole and carbamazepine. A high-performance liquid chromatography system was used to determine the removal of micropollutants. In the case of p-CBA, an ozone-resistant compound, the addition of catalyst was found to significantly enhance its degradation rate, leading to >99% removal under the optimum defined conditions, i.e., in terms of catalyst concentration, pH, temperature, and process time. On the other hand, in the case of atrazine, a different ozone-resistant compound, the introduction of examined catalysts in the ozonation process was found to reduce the degradation of micropollutant, when compared with the application of single ozonation, indicating the importance of specific affinity between the pollutant and the solid material used as catalyst. Benzotriazole, a moderately ozone-reactive compound was degraded by more than 95% under all experimental conditions and catalysts tested in the pilot unit, while carbamazepine, a highly ozone-reactive compound, was completely removed even during the first stage of treatment process (i.e., at the membrane contactor). When increasing the pH value (in the range 6–8) and the contact time, the performance of catalytic ozonation process also improved.
Role of Chemokine Cxcl12a in Mediating the Stimulatory Effects of Ethanol on Embryonic Development of Subpopulations of Hypocretin/Orexin Neurons and Their Projections
Authors: Yasmin Nushrat , Collier Adam D. , Abdulai Abdul R. , Karatayev Olga , Yu Boyi , Fam Milisia , Leibowitz Sarah F.
Published: May 2023

Studies in zebrafish and rats show that embryonic ethanol exposure at low-moderate concentrations stimulates hypothalamic neurons expressing hypocretin/orexin (Hcrt) that promote alcohol consumption, effects possibly involving the chemokine Cxcl12 and its receptor Cxcr4. Our recent studies in zebrafish of Hcrt neurons in the anterior hypothalamus (AH) demonstrate that ethanol exposure has anatomically specific effects on Hcrt subpopulations, increasing their number in the anterior AH (aAH) but not posterior AH (pAH), and causes the most anterior aAH neurons to become ectopically expressed further anterior in the preoptic area (POA). Using tools of genetic overexpression and knockdown, our goal here was to determine whether Cxcl12a has an important function in mediating the specific effects of ethanol on these Hcrt subpopulations and their projections. The results demonstrate that the overexpression of Cxcl12a has stimulatory effects similar to ethanol on the number of aAH and ectopic POA Hcrt neurons and the long anterior projections from ectopic POA neurons and posterior projections from pAH neurons. They also demonstrate that knockdown of Cxcl12a blocks these effects of ethanol on the Hcrt subpopulations and projections, providing evidence supporting a direct role of this specific chemokine in mediating ethanol’s stimulatory effects on embryonic development of the Hcrt system.
Reciprocal Effect of Environmental Stimuli to Regulate the Adipogenesis and Osteogenesis Fate Decision in Bone Marrow-Derived Mesenchymal Stem Cells (BM-MSCs)
Authors: Xu Xinyun , Zhao Ling , Terry Paul D. , Chen Jiangang
Published: May 2023

Mesenchymal stem cells derived from bone marrow (BM-MSCs) can differentiate into adipocytes and osteoblasts. Various external stimuli, including environmental contaminants, heavy metals, dietary, and physical factors, are shown to influence the fate decision of BM-MSCs toward adipogenesis or osteogenesis. The balance of osteogenesis and adipogenesis is critical for the maintenance of bone homeostasis, and the interruption of BM-MSCs lineage commitment is associated with human health issues, such as fracture, osteoporosis, osteopenia, and osteonecrosis. This review focuses on how external stimuli shift the fate of BM-MSCs towards adipogenesis or osteogenesis. Future studies are needed to understand the impact of these external stimuli on bone health and elucidate the underlying mechanisms of BM-MSCs differentiation. This knowledge will inform efforts to prevent bone-related diseases and develop therapeutic approaches to treat bone disorders associated with various pathological conditions.