bluuebunny/crossref_metadata_2025 huggingface dataset:
Mental Health Literacy in Zurich: A First Measurement Attempt Using the General HLS-EU-Q47
Authors: Schneider Michael , Jaks Rebecca , Nowak-Flück Daniela , Nicca Dunja , De Gani Saskia Maria
Published: September 2021

Background: Mental health literacy (MHL) promises to be an important factor for public health by enabling people to take responsibility for their own mental health. To date, there is no measurement tool that allows the assessment of a comprehensive understanding of MHL as part of health literacy (HL). Nonetheless, the widely used Health Literacy Survey European Questionnaire 47 (HLS-EU-Q47) includes items assessing at least some MHL-aspects in the context of HL. The present study aimed at investigating how these MHL-aspects are related to HL, health behavior and health outcome and how they differ between sociodemographic groups.

Methods: Data from the Health Literacy Survey Zurich 2018, collected by an adapted version of the HLS-EU-Q47, served to investigate these relationships.

Results: MHL-aspects were related to HL, health behavior and health outcome. Nearly half of all respondents (45%; N = 904) showed low MHL levels, particularly those with higher age and higher financial deprivation.

Conclusions: Relations of MHL-aspects with HL, health behavior, and health outcome indicate their potential importance for future interventions in public health, addressing mental health and MHL. A specific MHL tool is needed to comprehensively investigate these relations, which could be developed by extending the present measurement approach.
Household Socioeconomic Status and Antenatal Care Utilization Among Women in the Reproductive-Age
Authors: Sui Yubing , Ahuru Rolle Remi , Huang Kaishan , Anser Muhammad Khalid , Osabohien Romanus
Published: September 2021

The study examined the effect of household socioeconomic status and other socio-demographic characteristics on antenatal care (ANC) utilization among 819 women within the reproductive ages across eight rural communities in Delta State, Southern part of Nigeria. Characteristics of the women were described using simple proportion and frequency. The chi-square test was used to examine factors that were significantly associated with a minimum of four (≥4) and eight (≥8) antenatal care contacts, which were respectively in line with the focused ANC and WHO's new guideline. The multivariable logistic regression was used to examine the determinants of a minimum of four and eight ANC. Statistical analyses were set at 5%. The results showed that 31.4% (257/819) and 2.2% (18/819) of mothers, respectively, made ≥ 4 and ≥ 8 ANC contacts in the course of their last pregnancies. According to the results, the odds for reporting 4≥ and ≥ 8 ANC improved with both wealth and educational attainment. Distance to the health center and cost are barriers to maternal care utilization and they reduce the odds for undertaking ≥ 4 and ≥8 ANC contacts. Women on higher media exposure were more likely to undertake ≥ 4 and ≥8 ANC contacts, and those on the highest media exposure were more likely to undertake ≥8 ANC contacts. Financing maternal care through health insurance and free maternal care significantly improves the odds to undertake ≥ 4 and ≥ 8 ANC contacts. Intervention programs should be designed to improve access to maternal care services and should expand education opportunities for mothers, improve household socioeconomic conditions, and encourage enrolment in health insurance and free maternal care in the study area.
Pneumatic Soft Actuators With Kirigami Skins
Authors: Khosravi Hesameddin , Iannucci Steven M. , Li Suyi
Published: September 2021

Soft pneumatic actuators have become indispensable for many robotic applications due to their reliability, safety, and design flexibility. However, the currently available actuator designs can be challenging to fabricate, requiring labor-intensive and time-consuming processes like reinforcing fiber wrapping and elastomer curing. To address this issue, we propose to use simple-to-fabricate kirigami skins—plastic sleeves with carefully arranged slit cuts—to construct pneumatic actuators with pre-programmable motion capabilities. Such kirigami skin, wrapped outside a cylindrical balloon, can transform the volumetric expansion from pneumatic pressure into anisotropic stretching and shearing, creating a combination of axial extension and twisting in the actuator. Moreover, the kirigami skin exhibits out-of-plane buckling near the slit cut, which enables high stretchability. To capture such complex deformations, we formulate and experimentally validates a new kinematics model to uncover the linkage between the kirigami cutting pattern design and the actuator’s motion characteristics. This model uses a virtual fold and rigid-facet assumption to simplify the motion analysis without sacrificing accuracy. Moreover, we tested the pressure-stroke performance and elastoplastic behaviors of the kirigami-skinned actuator to establish an operation protocol for repeatable performance. Analytical and experimental parametric analysis shows that one can effectively pre-program the actuator’s motion performance, with considerable freedom, simply by adjusting the angle and length of the slit cuts. The results of this study can establish the design and analysis framework for a new family of kirigami-skinned pneumatic actuators for many robotic applications.
A Fish Story: Hot Water and Dangerous Behavior!
Authors: Davis Brittany E. , Sommer Ted , Fangue Nann A. , Todgham Anne E.
Published: September 2021

Climate change is warming up water all over the world, including in the San Francisco Estuary. This has caused fish who live there to change their behavior in unexpected ways. All animals, including fish, have regular and specialized behaviors that help them to survive. Fish swim, by themselves or in groups, to move to safer habitats, to find food or mates, or just to avoid danger. Scientists worry that changes to these behaviors brought on by warming water will make estuaries less safe for rare and endangered fish like the delta smelt. In the San Francisco Estuary, we found that warmer waters caused delta smelt to swim faster and further away from their neighbors, and to be eaten more often by predators. All these changes could hurt the ability of delta smelt to survive in the future.
@Indonesiatanpafeminis.id as a Challenge of Feminist Movement in Virtual Space
Authors: Maryani Eni , Janitra Preciosa Alnashava , Ratmita Reksa Anggia
Published: September 2021

The fast-growing social media in Indonesia has opened up opportunities for spreading feminist ideas to a wider and more diverse audience. Various social media accounts especially Instagram that focus on gender advocacy and feminism such as@indonesiafeminis, @lawanpatriarki, and @feminismanis have developed in Indonesia. However, the development of the social media platform also presents groups that oppose feminists. One of the accounts of women’s groups that oppose feminists is@indonesiatanpafeminis.id(@indonesiawithoutfeminist.id). The research objectives are namely to analyze the diversity of issues and reveal the discourse contestation that developed in the@indonesiatanpafeminis.id, and dynamic relationships on the online and offline spaces between groups of feminists and anti-feminists or the other interest. This research employed the digital ethnography method that utilized observation, interview, and literature study as data collection techniques. This study found that the online conversations at@indonesiatanpafeminis.idrevealed misconceptions on feminism from a group of women with a religious identity. Furthermore, the conversation also tends to strengthen patriarchal values with religious arguments that are gender-biased. However, the@indonesiatanpafeminis.idserves as a public space for open debates and education on feminist issues. The anti-feminist group behind the@indonesiatanpafeminis.idare women who identify themselves in a certain Muslim circle that has political, cultural, and religious agendas. One of the agendas is to influence the public to reject the Sexual Violence Eradication Bill. This study also noted the Muslim supporters of anti-feminism in Indonesia are less popular compared to progressive religious-based Muslim women organizations such as Aisyiyah (Muhammadiyah), Muslimat NU (Nahdlatul Ulama), and Rahima (Center for Education and Information on Islam and Women’s Rights). The study also evokes discussion on how the feminist and anti-feminist discourses can be utilized to criticize and develop the women’s movement or feminism in a multicultural context.
Swedish Sport Policy in an Era of Neoliberalism: An Expression of Social Entrepreneurship?
Authors: Bjärsholm Daniel , Norberg Johan R.
Published: September 2021

Since the turn of the millennium, Sweden has, like many other countries, become more neoliberal in many areas, including that of sport. The government has increased its expectations on the sports movement and become more result-oriented, which, for example, its revised motives for supporting the sports movement and the establishment of an audit organization can illustrate. However, in contrast to other countries, the Swedish government has not introduced any financial cutbacks in its support for sports. Rather, the opposite is true. The financial support has increased significantly over the last two decades. In the paper, we argue that this contradictory development of Swedish sport policy can be understood as expressions of neoliberalism and social entrepreneurship. As a theoretical concept, social entrepreneurship offers a way of understanding the increased Swedish government support for sport. There are in particular two underlying reasons for this claim. Firstly, sport is considered as a solution to various societal problems, such as social exclusion and refugee crises. Secondly, much of the increased support has been in form of various large-scale, earmarked, and time-limited political initiatives/reforms and project grants, which all have aimed to achieve social change through sport, such as social inclusion. In the paper, we consider these initiatives as expressions of social entrepreneurship. This paper contributes to the ongoing scholarly debate on how neoliberalism and neoliberal policies in the public sector have affected sport organizations. Also, by using social entrepreneurship theory, we provide new theoretical insights into how sport policy can be understood and analyzed.
Characterization of Subtype H6 Avian Influenza A Viruses Isolated From Wild Birds in Poyang Lake, China
Authors: Wan Zhimin , Kan Qiuqi , Zhao Zhehong , Shao Hongxia , Deliberto Thomas J. , Wan Xiu-Feng , Qin Aijian , Ye Jianqiang
Published: September 2021

Subtype H6 avian influenza A viruses (IAVs) are enzootic and genetically diverse in both domestic poultry and wild waterfowl and may cause spillovers in both pigs and humans. Thus, it is important to understand the genetic diversity of H6 IAVs in birds and their zoonotic potential. Compared with that in domestic poultry, the genetic diversity of H6 viruses in wild birds in China has not been well-understood. In this study, five H6 viruses were isolated from wild birds in Poyang Lake, China, and genetic analyses showed that these isolates are clustered into four genotypes associated with reassortments among avian IAVs from domestic poultry and wild birds in China and those from Eurasia and North America and that these viruses exhibited distinct phenotypes in growth kinetics analyses with avian and mammalian cells lines and in mouse challenge experiments. Of interest is that two H6 isolates from the Eurasian teal replicated effectively in the mouse lung without prior adaptation, whereas the other three did not. Our study suggested that there are variations in the mammalian viral replication efficiency phenotypic among genetically diverse H6 IAVs in wild birds and that both intra- and inter-continental movements of IAVs through wild bird migration may facilitate the emergence of novel H6 IAV reassortants with the potential for replicating in mammals, including humans. Continued surveillance to monitor the diversity of H6 IAVs in wild birds is necessary to increase our understanding of the natural history of IAVs.
Phylogeny and Pathogenicity of Phytophthora Species Associated with Artichoke Crown and Root Rot and Description of Phytophthora marrasii sp. nov.
Authors: Bregant Carlo , Rossetto Giovanni , Deidda Antonio , Maddau Lucia , Franceschini Antonio , Ionta Giorgio , Raiola Alessandro , Montecchio Lucio , Linaldeddu Benedetto T.
Published: September 2021

Field surveys conducted on nine farms over a 2-year period showed the widespread presence of Phytophthora-related diseases on globe artichoke plants in the main growing area in Sardinia (Italy). Characteristic symptoms included wilting and necrosis of the outermost leaves and dark brown discoloration of stem tissues, as well as root rot. A total of 18 Phytophthora colonies belonging to three species were isolated and characterized. Based on morphological features and ITS sequence data, Phytophthora isolates were identified as P. crassamura (eight isolates) and P. cactorum (four isolates). Six isolates could not be assigned to any formally described species of Phytophthora and are therefore described here as Phytophthora marrasii sp. nov. The ITS phylogeny places P. marrasii in a terminal clade basal to the sister taxa (P. foliorum, P. hibernalis, P. lateralis, and P. ramorum) of the clade 8c. In particular, P. marrasii is phylogenetically related to P. foliorum, a species from which it differs in 62 nucleotides in the ITS region. At the same time, it can easily be distinguished morphologically from P. foliorum mainly because of the low minimum temperature for growth, the bigger and persistent non-papillate sporangia, and smaller oogonia. Pathogenicity tests confirmed that all three Phytophthora species are pathogenic on globe artichokes, which represent a new host for these pathogens.
Quality Characteristics of Spelt Pasta Enriched with Spent Grain
Authors: Chetrariu Ancuța , Dabija Adriana
Published: September 2021

Agri-industrial by-products are valuable resources that can become ingredients for obtaining value-added products, thus supporting the circular economy. Spent grain is the primary by-product from the beer and whisky industry, is rich in fiber and protein, and can be successfully incorporated into pasta production. After dough properties were assessed, the resulting pasta was evaluated for chemical composition, phenolics content, as well as antioxidant activity. The cooked pasta quality was similarly evaluated on its physical properties, hardness, color profile of dry and hydrated pasta, and its sensory characteristics, as well as on the microstructure of the final products. Non-traditional ingredients such as spent grain and spelt flour influence the rheological properties of the dough and sensory acceptability and quality of the final cooked product. Spelt flour with the addition of spent grain can be used to obtain dry pasta of acceptable quality, with a high fiber content and biologically active compound, such as phenolic compounds. Using appropriate technologies, but also balanced recipes can incorporate suitable amounts of spent grain in pasta, resulting in final products characterized by desired dietary-nutritional values, as well as optimal sensory properties.
Potential Utilization of Diluted Seawater for the Cultivation of Some Summer Vegetable Crops: Physiological and Nutritional Implications
Authors: Javeed Hafiz Muhammad Rashad , Wang Xiukang , Ali Mazhar , Nawaz Fahim , Qamar Rafi , Rehman Atique ur , Shehzad Muhammad , Mubeen Muhammad , Shabbir Rubab , Javed Talha , Branca Ferdinando , Ahmar Sunny , Ismail Ismail A.
Published: September 2021

Freshwater alternatives to irrigate crops are in dire need of time because of changing climate and scarcity of freshwater. Therefore, seawater can be the best feasible option as it is abundantly available on Earth. Sole application of seawater for agricultural crops is impossible, but the blend with freshwater may not be aggravating the salinization problems in soils. The present investigation was conducted to evaluate the possibility of growing the short period summer vegetables i.e., eggplants, tomato, and pepper with fresh and seawater blends viz. 5%, 10% and 20%, termed as A, B and C treatments, respectively. During the experiment, we considered: (i) crop growth, biomass yield, water consumption, water use efficiency (WUE) and water productivity (WP); (ii) photosynthetic pigments and gas exchange parameters; (iii) concentration of mineral contents and quality traits; (iv) lipid peroxidation, proline contents and ascorbic acid. Tomato productivity was markedly decreased by application of B and C treatments, whereas the growth of eggplant and pepper were not much influenced at the same concentrations. Water consumption dropped, whereas WUE significantly increased in all tested crops upon increasing seawater concentrations. Leaf Na+ concentration and other mineral elements increased. These results assumed that certain concentrations of seawater–freshwater blends revealed that there were no significant effects on the quality characteristics, mineral elements concentration and productivity of eggplant and pepper plants; however, tomato plants indicated sensitivity at the three seawater concentrations (A, B and C SFW treatments). Moreover, low concentrations of salinity stress (5% and 10% SFW) seemed to be necessary to attain better crop nutrition and organoleptic values. Accordingly, the results of present will be helpful to coastline farmers in cultivating vegetables and produce nutritive food for their family.
Tracing Antibiotic Resistance Genes along the Irrigation Water Chain to Chive: Does Tap or Surface Water Make a Difference?
Authors: Gekenidis Maria-Theresia , Walsh Fiona , Drissner David
Published: September 2021

Irrigation water is well known as potential source of pathogens in fresh produce. However, its role in transferring antibiotic resistance determinants is less well investigated. Therefore, we analyzed the contribution of surface and tap water to the resistome of overhead-irrigated chive plants. Field-grown chive was irrigated with either surface water (R-system) or tap water (D-system), from planting to harvest. Water along the two irrigation chains as well as the respective plants were repeatedly sampled and screened for 264 antibiotic resistance genes (ARGs) and mobile genetic elements (MGEs), using high-capacity qPCR. Differentially abundant (DA) ARGs were determined by comparing the two systems. On R-chive, β-lactam ARGs, multidrug-resistance (MDR) determinants, and MGEs were most abundant, while D-chive featured DA ARGs from the vancomycin class. Diversity and number of DA ARGs was the highest on young chives, strongly diminished at harvest, and increased again at the end of shelf life. Most ARGs highly enriched on R- compared to D-chive were also enriched in R- compared to D-sprinkler water, indicating that water played a major role in ARG enrichment. Of note, blaKPC was detected at high levels in surface water and chive. We conclude that water quality significantly affects the resistome of the irrigated produce.
Saccharomyces Cerevisiae Var Boulardii CNCM I–1079 Reduces Expression of Genes Involved in Inflammatory Response in Porcine Cells Challenged by Enterotoxigenic E. Coli and Influences Bacterial Communities in an In Vitro Model of the Weaning Piglet Colon
Authors: Gresse Raphaële , Garrido Juan J. , Jiménez-Marín Angeles , Denis Sylvain , Van de Wiele Tom , Forano Evelyne , Blanquet-Diot Stéphanie , Chaucheyras-Durand Frédérique
Published: September 2021

Enterotoxigenic Escherichia coli (ETEC) is the main infectious agent responsible for piglet post-weaning diarrhea with high mortality rates. Antimicrobials represent the current principal strategy for treating ETEC infections in pig farms, but the occurrence of multi-resistant bacterial strains has considerably increased in the last decades. Thus, finding non-antibiotic alternatives becomes a real emergency. In this context, we investigated the effect of a live yeast strain, Saccharomyces cerevisiae var boulardii CNCM I-1079 (SB) in an in vitro model of the weaning piglet colon implemented with a mucus phase (MPigut-IVM) inoculated with ETEC and coupled with an intestinal porcine cell line IPI-2I. We showed that SB was able to modulate the in vitro microbiota through an increase in Bacteroidiaceae and a decrease in Prevotellaceae families. Effluents collected from the SB treated bioreactors were able to mitigate the expression level of genes encoding non-gel forming mucins, tight junction proteins, innate immune pathway, and pro-inflammatory response in IPI-2I cells. Furthermore, SB exerted a significant protective effect against ETEC adhesion on porcine IPEC-J2 intestinal cells in a dose-dependent manner and showed a positive effect on ETEC-challenged IPEC-J2 by lowering expression of genes involved in pro-inflammatory immune responses. Our results showed that the strain SB CNCM I-1079 could prevent microbiota dysbiosis associated with weaning and protect porcine enterocytes from ETEC infections by reducing bacterial adhesion and modulating the inflammatory response.
Verbascoside Protects Gingival Cells against High Glucose-Induced Oxidative Stress via PKC/HMGB1/RAGE/NFκB Pathway
Authors: Hsieh Pei-Fang , Yu Cheng-Chia , Chu Pei-Ming , Hsieh Pei-Ling
Published: September 2021

Impaired wound healing often occurs in patients with diabetes and causes great inconvenience to them. Aside from the presence of prolonged inflammation, the accumulation of oxidative stress is also implicated in the delayed wound healing. In the present study, we tested the effect of verbascoside, a caffeoyl phenylethanoid glycoside, on the improvement of cell viability and wound healing capacity of gingival epithelial cells under high glucose condition. We showed that verbascoside attenuated the high glucose-induced cytotoxicity and impaired healing, which may be associated with the downregulation of oxidative stress. Our results demonstrated that verbascoside increased the activity of the antioxidant enzyme SOD and reduced the oxidative stress indicator, 8-OHdG, as well as apoptosis. Moreover, verbascoside upregulated the PGC1-α and NRF1 expression and promoted mitochondrial biogenesis, which was mediated by suppression of PKC/HMGB1/RAGE/NFκB signaling. Likewise, we showed the inhibitory effect of verbascoside on oxidative stress was via repression of PKC/HMGB1/RAGE/NFκB activation. Also, our data suggested that the PKC-mediated oxidative stress may lead to the elevated production of inflammatory cytokines, IL-6 and IL-1β. Collectively, we demonstrated that verbascoside may be beneficial to ameliorate impaired oral wound healing for diabetic patients.
Holding Force and Vertical Vibration of Emergency Gate in the Closing Process: Physical and Numerical Modelling
Authors: Wang Yanzhao , Xu Guobin , Liu Fang
Published: September 2021

A two-dimensional unsteady fluid–structure interaction numerical model was established, based on the physical model test, to investigate the influence of vertical vibration on the holding force of an emergency gate in the closing process. Gate motion was controlled by the user-defined function in Fluent. Attention was paid to the relationship between the vertical vibration, hydrodynamic loads and flow discharge. The experiment results show that holding force has three typical forms in the closing process and it is related to the service gate height. The numerical model can reflect the gate vertical vibration and the gate-closing displacement in the form of steps. Gate vertical vibration in the closing process is a motion-induced vibration caused by gate active falling. Moreover, the transition from full-flow to open-flow behind the emergency gate has a great influence on the gate vertical vibration. With a small gate opening, gate vertical vibration makes the flow discharge fluctuation increase. Furthermore, flow discharge has an influence on the gate body loads, which is mainly concentrated in the upstream plate and gate bottom. Finally, the lift force coefficient at the gate bottom is different from the standard and is mainly controlled by the outflow boundary condition. The simulation result is in good agreement with the experiment and the relative error meets engineering requirements, suggesting that the numerical model can successfully simulate the gate fluid–structure interaction and reproduce the characteristics of physical quantities in the closing process.
E-Nose and Olfactory Assessment: Teamwork or a Challenge to the Last Data? The Case of Virgin Olive Oil Stability and Shelf Life
Authors: Modesti Margherita , Taglieri Isabella , Bianchi Alessandro , Tonacci Alessandro , Sansone Francesco , Bellincontro Andrea , Venturi Francesca , Sanmartin Chiara
Published: September 2021

Electronic nose (E-nose) devices represent one of the most trailblazing innovations in current technological research, since mimicking the functioning of the biological sense of smell has always represented a fascinating challenge for technological development applied to life sciences and beyond. Sensor array tools are right now used in a plethora of applications, including, but not limited to, (bio-)medical, environmental, and food industry related. In particular, the food industry has seen a significant rise in the application of technological tools for determining the quality of edibles, progressively replacing human panelists, therefore changing the whole quality control chain in the field. To this end, the present review, conducted on PubMed, Science Direct and Web of Science, screening papers published between January 2010 and May 2021, sought to investigate the current trends in the usage of human panels and sensorized tools (E-nose and similar) in the food industry, comparing the performances between the two different approaches. In particular, the focus was mainly addressed towards the stability and shelf life assessment of olive oil, the main constituent of the renowned “Mediterranean diet”, and nowadays appreciated in cuisines from all around the world. The obtained results demonstrate that, despite the satisfying performances of both approaches, the best strategy merges the potentialities of human sensory panels and technological sensor arrays, (i.e., E-nose somewhat supported by E-tongue and/or E-eye). The current investigation can be used as a reference for future guidance towards the choice between human panelists and sensorized tools, to the benefit of food manufacturers.
Removal of Nitrate Ions Using Thermally and Chemically Modified Bioadsorbents
Authors: Tejada-Tovar Candelaria , Villabona-Ortíz Ángel , Gonzalez-Delgado Ángel Darío
Published: September 2021

Nitrate ions are one of the causes of surface water eutrophication. In the present research, the effective adsorption of nitrate ions (NO3) on bioadsorbents prepared from yam skins (Dioscorea alata) was evaluated. The yam skin was chemically modified with ammonium chloride (NH4Cl), and biochar was prepared from the thermally modified biomass. The results were compared with commercial coal (CC). The bioadsorbents were characterized by SEM-EDS analysis, which showed that the synthesized adsorbents have a heterogeneous surface with pores. The batch adsorption tests showed that the pH has the most significant effect on the NO3 adsorption capacity when using the modified yam skin (MYP), obtaining as best conditions pH 12 and a 0.5 mm particle size with an adsorption capacity of 25.75 mg/g; the best adsorption capacity when using the carbon synthesized from yam skin (CYP) and CC was obtained at pH 2, reaching values of 36 and 33.34 mg/g, respectively. The following performance in terms of adsorption capacity was found: CYP > CC > MYP, according to Langmuir’s model. The equilibrium isotherm of NO3 adsorption on MYP and CYP was adjusted by the Freundlich model, while the Langmuir model described adsorption on CC. The kinetics of all the systems studied showed a good fit to the pseudo-first-order, pseudo-second-order and Elovich models with R2 > 0.95, suggesting a mechanism of chemical adsorption by means of species exchange between the aqueous phase and the surface of the material, with the intervention of an intraparticle diffusion stage; based on these findings, the studied biomasses reached promising adsorption capacities in the removal of nitrate anions, showing that the carbon synthesized from yam skins and modified with ammonium chloride had a remarkable behavior in comparison with commercial carbon and NH4Cl-modified yam skins.
Experimental Investigation of Concrete Transverse Deformations at Relatively High Loading Rates for Interpretation of High Strength Concrete Behavior
Authors: Iskhakov Iakov , Frolov Ilya , Ribakov Yuri
Published: September 2021

Loading rates affect the behavior of concrete specimens from the beginning of the loading process until failure. At rather high loading rates, longitudinal deformations in concrete specimens under a compressive load are practically elastic up until the ultimate limit state. It has been previously demonstrated that transverse deformations effectively indicate high-strength concrete behavior in the entire static loading process range. A theoretical model for cylindrical concrete specimen failure under compressive load, based on a structural phenomenon, has also been proposed. The aim of the present research is experimental verification of using transverse deformations in addition to longitudinal ones for investigating high-strength concrete behavior at the non-elastic stage. This research is based on testing normal-strength concrete cylindrical specimens under compression at relatively high loading rates. The theoretical model of the cracking and failure scheme of the cylindrical specimens are experimentally confirmed. The obtained results demonstrate that it is possible to use transverse deformations for the interpretation of initiation and development of inelastic deformations in high-strength concrete up to class C90 based on the data for normal-strength concrete specimens of class C30 subjected to relatively high loading rates.
Bioinformatics and Machine Learning Approaches to Understand the Regulation of Mobile Genetic Elements
Authors: Giassa Ilektra-Chara , Alexiou Panagiotis
Published: September 2021

Transposable elements (TEs, or mobile genetic elements, MGEs) are ubiquitous genetic elements that make up a substantial proportion of the genome of many species. The recent growing interest in understanding the evolution and function of TEs has revealed that TEs play a dual role in genome evolution, development, disease, and drug resistance. Cells regulate TE expression against uncontrolled activity that can lead to developmental defects and disease, using multiple strategies, such as DNA chemical modification, small RNA (sRNA) silencing, chromatin modification, as well as sequence-specific repressors. Advancements in bioinformatics and machine learning approaches are increasingly contributing to the analysis of the regulation mechanisms. A plethora of tools and machine learning approaches have been developed for prediction, annotation, and expression profiling of sRNAs, for methylation analysis of TEs, as well as for genome-wide methylation analysis through bisulfite sequencing data. In this review, we provide a guided overview of the bioinformatic and machine learning state of the art of fields closely associated with TE regulation and function.
Trichoderma asperellum Secreted 6-Pentyl-α-Pyrone to Control Magnaporthiopsis maydis, the Maize Late Wilt Disease Agent
Authors: Degani Ofir , Khatib Soliman , Becher Paz , Gordani Asaf , Harris Raviv
Published: September 2021

Late wilt disease (LWD) is a destructive vascular disease of maize (Zea mays L.) caused by the fungus Magnaporthiopsis maydis. Restricting the disease, which is a significant threat to commercial production in Israel, Egypt, Spain, India, and other countries, is an urgent need. In the past three years, we scanned nine Trichoderma spp. isolates as biological control candidates against M. maydis. Three of these isolates showed promising results. In vitro assays, seedlings pathogenicity trials, and field experiments all support the bio-control potential of these isolates (or their secretions). Here, a dedicated effort led to the isolation and identification of an active ingredient in the growth medium of Trichoderma asperellum (P1) with antifungal activity against M. maydis. This Trichoderma species is an endophyte isolated from LWD-susceptible maize seeds. From the chloroform extract of this fungal medium, we isolated a powerful (approx. 400 mg/L) active ingredient capable of fully inhibiting M. maydis growth. Additional purification using liquid chromatography–mass spectrometry (LC–MS) and gas chromatography–mass spectrometry (GC–MS) separation steps enabled identifying the active ingredient as 6-Pentyl-α-pyrone. This compound is a potential fungicide with high efficiency against the LWD causal agent.
Low SARS-CoV-2 Seroprevalence and No Active Infections among Dogs and Cats in Animal Shelters with Laboratory-Confirmed COVID-19 Human Cases among Employees
Authors: Cossaboom Caitlin M. , Medley Alexandra M. , Spengler Jessica R. , Kukielka Esther A. , Goryoka Grace W. , Baird Tiffany , Bhavsar Swity , Campbell Stefanie , Campbell Thomas S. , Christensen Daniel , Condrey Jillian A. , Dawson Patrick , Doty Jeffrey B. , Feldpausch Amanda , Gabel Julie , Jones Dee , Lim Ailam , Loiacono Christina M. , Jenkins-Moore Melinda , Moore Andrea , Noureddine Clarissa , Ortega Jorge , Poulsen Keith , Rooney Jane A. , Rossow John , Sheppard Karen , Sweet Emma , Stoddard Robyn , Tell Rachel M. , Wallace Ryan M. , Williams Carl , Barton Behravesh Casey
Published: September 2021

Human-to-animal and animal-to-animal transmission of SARS-CoV-2 has been documented; however, investigations into SARS-CoV-2 transmission in congregate animal settings are lacking. We investigated four animal shelters in the United States that had identified animals with exposure to shelter employees with laboratory-confirmed COVID-19. Of the 96 cats and dogs with specimens collected, only one dog had detectable SARS-CoV-2 neutralizing antibodies; no animal specimens had detectable viral RNA. These data indicate a low probability of human-to-animal transmission events in cats and dogs in shelter settings with early implementation of infection prevention interventions.
Peer Presence Effect on Numerosity and Phonological Comparisons in 4th Graders: When Working with a SchoolMate Makes Children More Adult-like
Authors: Tricoche Leslie , Monfardini Elisabetta , Reynaud Amélie J. , Epinat-Duclos Justine , Pélisson Denis , Prado Jérôme , Meunier Martine
Published: September 2021

Little is known about how peers’ mere presence may, in itself, affect academic learning and achievement. The present study addresses this issue by exploring whether and how the presence of a familiar peer affects performance in a task assessing basic numeracy and literacy skills: numerosity and phonological comparisons. We tested 99 fourth-graders either alone or with a classmate. Ninety-seven college-aged young adults were also tested on the same task, either alone or with a familiar peer. Peer presence yielded a reaction time (RT) speedup in children, and this social facilitation was at least as important as that seen in adults. RT distribution analyses indicated that the presence of a familiar peer promotes the emergence of adult-like features in children. This included shorter and less variable reaction times (confirmed by an ex-Gaussian analysis), increased use of an optimal response strategy, and, based on Ratcliff’s diffusion model, speeded up nondecision (memory and/or motor) processes. Peer presence thus allowed children to at least narrow (for demanding phonological comparisons), and at best, virtually fill in (for unchallenging numerosity comparisons) the developmental gap separating them from adult levels of performance. These findings confirm the influence of peer presence on skills relevant to education and lay the groundwork for exploring how the brain mechanisms mediating this fundamental social influence evolve during development.
Influence of Lactic Acid Surface Modification of Cellulose Nanofibrils on the Properties of Cellulose Nanofibril Films and Cellulose Nanofibril–Poly(lactic acid) Composites
Authors: Lafia-Araga Ruth Anayimi , Sabo Ronald , Nabinejad Omid , Matuana Laurent , Stark Nicole
Published: September 2021

In this study, cellulose nanofibrils (CNFs) were modified by catalyzed lactic acid esterification in an aqueous medium with SnCl2 as a catalyst. Films were made from unmodified and lactic acid-modified CNF without a polymer matrix to evaluate the effectiveness of the modification. Ungrafted and lactic acid-grafted CNF was also compounded with poly(lactic acid) (PLA) to produce composites. Mechanical, water absorption, and barrier properties were evaluated for ungrafted CNF, lactic acid-grafted CNF films, and PLA/CNF composites to ascertain the effect of lactic acid modification on the properties of the films and nanocomposites. FTIR spectra of the modified CNF revealed the presence of carbonyl peaks at 1720 cm−1, suggesting that the esterification reaction was successful. Modification of CNF with LA improved the tensile modulus of the produced films but the tensile strength and elongation decreased. Additionally, films made from modified CNF had lower water absorption, as well as water vapor and oxygen permeability, relative to their counterparts with unmodified CNFs. The mechanical properties of PLA/CNF composites made from lactic acid-grafted CNFs did not significantly change with respect to the ungrafted CNF. However, the addition of lactic acid-grafted CNF to PLA improved the water vapor permeability relative to composites containing ungrafted CNF. Therefore, the esterification of CNFs in an aqueous medium may provide an environmentally benign way of modifying the surface chemistry of CNFs to improve the barrier properties of CNF films and PLA/CNF composites.
Theory and Practice of Coarse-Grained Molecular Dynamics of Biologically Important Systems
Authors: Liwo Adam , Czaplewski Cezary , Sieradzan Adam K. , Lipska Agnieszka G. , Samsonov Sergey A. , Murarka Rajesh K.
Published: September 2021

Molecular dynamics with coarse-grained models is nowadays extensively used to simulate biomolecular systems at large time and size scales, compared to those accessible to all-atom molecular dynamics. In this review article, we describe the physical basis of coarse-grained molecular dynamics, the coarse-grained force fields, the equations of motion and the respective numerical integration algorithms, and selected practical applications of coarse-grained molecular dynamics. We demonstrate that the motion of coarse-grained sites is governed by the potential of mean force and the friction and stochastic forces, resulting from integrating out the secondary degrees of freedom. Consequently, Langevin dynamics is a natural means of describing the motion of a system at the coarse-grained level and the potential of mean force is the physical basis of the coarse-grained force fields. Moreover, the choice of coarse-grained variables and the fact that coarse-grained sites often do not have spherical symmetry implies a non-diagonal inertia tensor. We describe selected coarse-grained models used in molecular dynamics simulations, including the most popular MARTINI model developed by Marrink’s group and the UNICORN model of biological macromolecules developed in our laboratory. We conclude by discussing examples of the application of coarse-grained molecular dynamics to study biologically important processes.
New Orders to an Old Soldier: Optimizing NK Cells for Adoptive Immunotherapy in Hematology
Authors: Gunduz Mehmet , Ataca Atilla Pinar , Atilla Erden
Published: September 2021

NK (Natural Killer) cell-mediated adoptive immunotherapy has gained attention in hematology due to the progressing knowledge of NK cell receptor structure, biology and function. Today, challenges related to NK cell expansion and persistence in vivo as well as low cytotoxicity have been mostly overcome by pioneering trials that focused on harnessing NK cell functions. Recent technological advancements in gene delivery, gene editing and chimeric antigen receptors (CARs) have made it possible to generate genetically modified NK cells that enhance the anti-tumor efficacy and represent suitable “off-the-shelf” products with fewer side effects. In this review, we highlight recent advances in NK cell biology along with current approaches for potentiating NK cell proliferation and activity, redirecting NK cells using CARs and optimizing the procedure to manufacture clinical-grade NK and CAR NK cells for adoptive immunotherapy.
Recent Advances in Hydrogels: Ophthalmic Applications in Cell Delivery, Vitreous Substitutes, and Ocular Adhesives
Authors: Lin Kenny T. , Wang Athena , Nguyen Alexandra B. , Iyer Janaki , Tran Simon D.
Published: September 2021

With the prevalence of eye diseases, such as cataracts, retinal degenerative diseases, and glaucoma, different treatments including lens replacement, vitrectomy, and stem cell transplantation have been developed; however, they are not without their respective shortcomings. For example, current methods to seal corneal incisions induced by cataract surgery, such as suturing and stromal hydration, are less than ideal due to the potential for surgically induced astigmatism or wound leakage. Vitrectomy performed on patients with diabetic retinopathy requires an artificial vitreous substitute, with current offerings having many shortcomings such as retinal toxicity. The use of stem cells has also been investigated in retinal degenerative diseases; however, an optimal delivery system is required for successful transplantation. The incorporation of hydrogels into ocular therapy has been a critical focus in overcoming the limitations of current treatments. Previous reviews have extensively documented the use of hydrogels in drug delivery; thus, the goal of this review is to discuss recent advances in hydrogel technology in surgical applications, including dendrimer and gelatin-based hydrogels for ocular adhesives and a variety of different polymers for vitreous substitutes, as well as recent advances in hydrogel-based retinal pigment epithelium (RPE) and retinal progenitor cell (RPC) delivery to the retina.
The Effects of Simulated Microgravity on Macrophage Phenotype
Authors: Ludtka Christopher , Moore Erika , Allen Josephine B.
Published: September 2021

The effects of spaceflight, including prolonged exposure to microgravity, can have significant effects on the immune system and human health. Altered immune cell function can lead to adverse health events, though precisely how and to what extent a microgravity environment impacts these cells remains uncertain. Macrophages, a key immune cell, effect the inflammatory response as well as tissue remodeling and repair. Specifically, macrophage function can be dictated by phenotype that can exist between spectrums of M0 macrophage: the classically activated, pro-inflammatory M1, and the alternatively activated, pro-healing M2 phenotypes. This work assesses the effects of simulated microgravity via clinorotation on M0, M1, and M2 macrophage phenotypes. We focus on phenotypic, inflammatory, and angiogenic gene and protein expression. Our results show that across all three phenotypes, microgravity results in a decrease in TNF-α expression and an increase in IL-12 and VEGF expression. IL-10 was also significantly increased in M1 and M2, but not M0 macrophages. The phenotypic cytokine expression profiles observed may be related to specific gravisensitive signal transduction pathways previously implicated in microgravity regulation of macrophage gene and protein expression. Our results highlight the far-reaching effects that simulated microgravity has on macrophage function and provides insight into macrophage phenotypic function in microgravity.
A Therapeutic Matrix: Virtual Reality as a Clinical Tool for Spinal Cord Injury-Induced Neuropathic Pain
Authors: Leemhuis Erik , Giuffrida Valentina , Giannini Anna Maria , Pazzaglia Mariella
Published: September 2021

Neuropathic pain (NP) is a chronic, debilitating, and resistant form of pain. The onset rate of NP following spinal cord injuries (SCI) is high and may reduce the quality of life more than the sensorimotor loss itself. The long-term ineffectiveness of current treatments in managing symptoms and counteracting maladaptive plasticity highlights the need to find alternative therapeutic approaches. Virtual reality (VR) is possibly the best way to administer the specific illusory or reality-like experience and promote behavioral responses that may be effective in mitigating the effects of long-established NP. This approach aims to promote a more systematic adoption of VR-related techniques in pain research and management procedures, highlighting the encouraging preliminary results in SCI. We suggest that the multisensory modulation of the sense of agency and ownership by residual body signals may produce positive responses in cases of brain-body disconnection. First, we focus on the transversal role embodiment and how multisensory and environmental or artificial stimuli modulate illusory sensations of bodily presence and ownership. Then, we present a brief overview of the use of VR in healthcare and pain management. Finally, we discus research experiences which used VR in patients with SCI to treating NP, including the most recent combinations of VR with further stimulation techniques.
Synthesis of Semicrystalline Long Chain Aliphatic Polyesters by ADMET Copolymerization of Dianhydro-D-glucityl bis(undec-10-enoate) with 1,9-Decadiene and Tandem Hydrogenation
Authors: Kojima Mika , Abdellatif Mohamed Mehawed , Nomura Kotohiro
Published: September 2021

Acyclic diene metathesis (ADMET) copolymerization of dianhydro-D-glucityl bis(undec-10-enoate) (M1) with 1,9-decadiene (DCD) using ruthenium-carbene catalyst, RuCl2(IMesH2)(CH-2-OiPr-C6H4) [IMesH2 = 1,3-bis(2,4,6-trimethylphenyl)imidazolin-2-ylidene, HG2], afforded unsaturated polyesters (Mn = 9300–23,400) under the optimized conditions. Subsequent tandem hydrogenation (H2 1.0 MPa, 50 °C) with the addition of a small amount of Al2O3 resulted in the saturated polymers having a melting temperature of 71.7–107.6 °C, depending on the molar ratio of M1 and DCD.
Longitudinal Follow-Up of Blood Telomere Length in HIV-Exposed Uninfected Children Having Received One Year of Lopinavir/Ritonavir or Lamivudine as Prophylaxis
Authors: Monnin Audrey , Vizeneux Amélie , Nagot Nicolas , Eymard-Duvernay Sabrina , Meda Nicolas , Singata-Madliki Mandisa , Ndeezi Grace , Tumwine James Kashugyera , Kankasa Chipepo , Goga Ameena , Tylleskär Thorkild , Van de Perre Philippe , Molès Jean-Pierre
Published: September 2021

Telomere shortening can be enhanced upon human immunodeficiency virus (HIV) infection and by antiretroviral (ARV) exposures. The aim of this study was to evaluate the acute and long-term effect on telomere shortening of two ARV prophylaxes, lopinavir/ritonavir (LPV/r) and lamivudine (3TC), administered to children who are HIV-exposed uninfected (CHEU) to prevent HIV acquisition through breastfeeding during the first year of life, and to investigate the relationship between telomere shortening and health outcomes at six years of age. We included 198 CHEU and measured telomere length at seven days of life, at week-50 and at six years (year-6) using quantitative polymerase chain reaction. At week-50, telomere shortening was observed among 44.3% of CHEU, irrespective of the prophylactic treatment. Furthermore, this telomere shortening was neither associated with poor growth indicators nor neuropsychological outcomes at year-6, except for motor abilities (MABC test n = 127, β = −3.61, 95%CI: −7.08, −0.14; p = 0.04). Safety data on telomere shortening for infant HIV prophylaxis are scarce. Its association with reduced motor abilities deserves further attention among CHEU but also HIV-infected children receiving ARV treatment.