bluuebunny/crossref_metadata_2025 huggingface dataset:
A Methodology for Increasing Convergence Speed of Traffic Assignment Algorithms Based on the Use of a Generalised Averaging Function
Authors: Botte Marilisa , Gallo Mariano , Marinelli Mario , D’Acierno Luca
Published: August 2020

In this paper, we propose a generalisation of the Method of Successive Averages (MSA) for solving traffic assignment problems. The generalisation consists in proposing a different step sequence within the general MSA framework to reduce computing times. The proposed step sequence is based on the modification of the classic 1/k sequence for improving the convergence speed of the algorithm. The reduction in computing times is useful if the assignment problems are subroutines of algorithms for solving Network Design Problems—such algorithms require estimation of the equilibrium traffic flows at each iteration, hence, many thousands of times for real-scale cases. The proposed algorithm is tested with different parameter values and compared with the classic MSA algorithm on a small and on two real-scale networks. A test inside a Network Design Problem is also reported. Numerical results show that the proposed algorithm outperforms the classic MSA with reductions in computing times, reaching up to 79%. Finally, the theoretical properties are studied for stating the convergence of the proposed algorithm.
An Improved Method for Optical Characterization of Mineral Dust and Soot Particles in the El Paso-Juárez Airshed
Authors: Polanco Javier , Ramos Manuel , Fitzgerald Rosa M. , Stockwell William R.
Published: August 2020

Highly time-resolved aerosol measurements and analysis are necessary for a proper aerosol characterization in many polluted regions, because aerosol concentrations in polluted environments can change over time scales of minutes. However, many urban measuring sites have measuring devices that provide time resolved average aerosol concentrations over a day or two at best. Light-scattering properties of mineral dust and soot particles in the El Paso-Juárez Airshed were analyzed with an improved methodology, using the T-matrix, a maximum likelihood estimator (MLE), and data from both an acoustic extinctiometer and a laser particle counter. The hourly inter-comparisons of the scattering coefficients’ results between the model and those obtained using the instruments at a wavelength of 0.87 μm show good agreement. This methodology has been applied in the El Paso-Juárez Airshed successfully, and it could be used in other cities where mineral dust and soot are major components of the aerosol concentrations.
Health Effects of Air-Quality Regulations in Seoul Metropolitan Area: Applying Synthetic Control Method to Controlled-Interrupted Time-Series Analysis
Authors: Kim Soo-Yeon , Kim Hyomi , Lee Jong-Tae
Published: August 2020

Despite enormous investment in air-quality regulations, there are only a few studies about the health effects of the air-quality regulations. By applying synthetic control methods to controlled-interrupted time-series analysis, this study aimed to test whether air-quality regulations implemented in Seoul metropolitan area since 2005 had reduced cardiovascular mortality rate in Seoul and Incheon. Each synthetic control for Seoul and Incheon was constructed to predict the counterfactual cardiovascular mortality rate through synthetic control methods. By using a synthetic control as a control group in controlled-interrupted time-series analysis, we tested whether the air-quality regulations had changed the trend of cardiovascular mortality rate in Seoul and Incheon after the intervention. The results showed a significant slope change in cardiovascular mortality rate in Seoul (coefficient: −0.001, 95% confidence interval (CI): −0.0015, −0.0004) and Incheon (coefficient: −0.0006, 95% CI: −0.0012, 0). This study suggests additional evidence that air-quality regulations implemented in the Seoul metropolitan areas since 2005 had beneficial effects on cardiovascular mortality rate in Seoul and Incheon.
Functional Beer—A Review on Possibilities
Authors: Habschied Kristina , Živković Antonio , Krstanović Vinko , Mastanjević Krešimir
Published: August 2020

The expansion of the beer industry has enabled many possibilities for improvement regarding the taste, aroma and functionality of this drink. Health-related issues and a general wish for healthier lifestyles has resulted in increased demand for functional beers. The addition of different herbs or adjuncts in wort or beer has been known for centuries. However, today’s technologies provide easier ways to do this and offer additional functional properties for the health benefits and sensory adjustments of classical beer. Medicinal, religious or trendy reasons for avoiding certain compounds in beer or the need to involve new ones in the brewing recipe has broadened the market for the brewing industry and made beer more accessible to consumers who, till now, avoided beer.
Introduction to the Special Issue on Advances in Biological Tissue Biomechanics
Authors: Lee Chung-Hao , Liao Jun
Published: August 2020

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Physioxia Expanded Bone Marrow Derived Mesenchymal Stem Cells Have Improved Cartilage Repair in an Early Osteoarthritic Focal Defect Model
Authors: Pattappa Girish , Krueckel Jonas , Schewior Ruth , Franke Dustin , Mench Alexander , Koch Matthias , Weber Johannes , Lang Siegmund , Pfeifer Christian G. , Johnstone Brian , Docheva Denitsa , Alt Volker , Angele Peter , Zellner Johannes
Published: August 2020

Focal early osteoarthritis (OA) or degenerative lesions account for 60% of treated cartilage defects each year. The current cell-based regenerative treatments have an increased failure rate for treating degenerative lesions compared to traumatic defects. Mesenchymal stem cells (MSCs) are an alternative cell source for treating early OA defects, due to their greater chondrogenic potential, compared to early OA chondrocytes. Low oxygen tension or physioxia has been shown to enhance MSC chondrogenic matrix content and could improve functional outcomes of regenerative therapies. The present investigation sought to develop a focal early OA animal model to evaluate cartilage regeneration and hypothesized that physioxic MSCs improve in vivo cartilage repair in both, post-trauma and focal early OA defects. Using a rabbit model, a focal defect was created, that developed signs of focal early OA after six weeks. MSCs cultured under physioxia had significantly enhanced in vitro MSC chondrogenic GAG content under hyperoxia with or without the presence of interleukin-1β (IL-1β). In both post-traumatic and focal early OA defect models, physioxic MSC treatment demonstrated a significant improvement in cartilage repair score, compared to hyperoxic MSCs and respective control defects. Future investigations will seek to understand whether these results are replicated in large animal models and the underlying mechanisms involved in in vivo cartilage regeneration.
Naphthoquinone-Based Meroterpenoids from Marine-Derived Streptomyces sp. B9173
Authors: Shen Xinqian , Wang Xiaozheng , Huang Tingting , Deng Zixin , Lin Shuangjun
Published: August 2020

Naphthoquinone-based meroterpenoids are hybrid polyketide-terpenoid natural products with chemical diversity and a broad range of biological activities. Here, we report the isolation of a group of naphthoquinone-containing compounds from Streptomyces sp. B9173, and their structures were elucidated by using a combination of spectroscopic techniques, including 1D, 2D NMR, and high-resolution mass (HRMS) analysis. Seven flaviogeranin congeners or intermediates, three of which were new, have been derived from common naphthoquinone backbone and subsequent oxidation, methylation, prenylation, and amino group incorporation. Both flaviogeranin B1 (1) and B (2) contain an amino group which was incorporated into the C8 of 1,3,6,8-terhydroxynaphthalene (THN). Flaviogeranin D (3) contains an intact C-geranylgeranyl residue attached to the C2 of THN, while the O-geranylgeranyl group of 2 links with the hydroxyl on the C2 site of THN. Four compounds were selected and tested for antibacterial activity and cytotoxicity, with 3 and flaviogeranin C2 (5) displaying potent activity against selected bacteria and cancer cell lines. In light of the structure features of isolated compounds and the biosynthetic genes, a biosynthetic pathway of naphthoquinone-based flaviogeranins has been proposed. These isolated compounds not only extend the structural diversity but also represent new insights into the biosynthesis of naphthoquinone-based meroterpenoids.
Free-Radical-Mediated Formation of Trans-Cardiolipin Isomers, Analytical Approaches for Lipidomics and Consequences of the Structural Organization of Membranes
Authors: Vetica Fabrizio , Sansone Anna , Meliota Cesare , Batani Gessica , Roberti Marinella , Chatgilialoglu Chryssostomos , Ferreri Carla
Published: August 2020

Free-radical-mediated processes, such as peroxidation, isomerization and hydrogenation affecting fatty acid integrity and biological functions, have a trans-disciplinary relevance. Cardiolipins (CL, (1,3-diphosphatidyl-sn-glycerol)) and tetra-linoleoyl-CL are complex phospholipids, exclusively present in the Inner Mitochondrial Membrane (IMM) lipids, where they maintain membrane integrity and regulate enzyme functionalities. Peroxidation pathways and fatty acid remodeling are known causes of mitochondrial disfunctions and pathologies, including cancer. Free-radical-mediated isomerization with the change of the cis CL into geometrical trans isomers is an unknown process with possible consequences on the supramolecular membrane lipid organization. Here, the formation of mono-trans CL (MT-CL) and other trans CL isomers (T-CL) is reported using CL from bovine heart mitochondria and thiyl radicals generated by UV-photolysis from 2-mercaptoethanol. Analytical approaches for CL isomer separation and identification via 1H/13C NMR are provided, together with the chemical study of CL derivatization to fatty acid methyl esters (FAME), useful for lipidomics and metabolomics research. Kinetics information of the radical chain isomerization process was obtained using γ-irradiation conditions. The CL isomerization affected the structural organization of membranes, as tested by the reduction in unilamellar liposome diameter, and accompanied the well-known process of oxidative consumption induced by Fenton reagents. These results highlight a potential new molecular modification pathway of mitochondrial lipids with wide applications to membrane functions and biological consequences.
R-α-Lipoic Acid and 4-Phenylbutyric Acid Have Distinct Hypolipidemic Mechanisms in Hepatic Cells
Authors: He Bo , Moreau Régis
Published: August 2020

The constitutive activation of the mechanistic target of rapamycin complex 1 (mTORC1) leads to the overproduction of apoB-containing triacylglycerol-rich lipoproteins in HepG2 cells. R-α-lipoic acid (LA) and 4-phenylbutyric acid (PBA) have hypolipidemic function but their mechanisms of action are not well understood. Here, we reported that LA and PBA regulate hepatocellular lipid metabolism via distinct mechanisms. The use of SQ22536, an inhibitor of adenylyl cyclase, revealed cAMP’s involvement in the upregulation of CPT1A expression by LA but not by PBA. LA decreased the secretion of proprotein convertase subtilisin/kexin type 9 (PCSK9) in the culture media of hepatic cells and increased the abundance of LDL receptor (LDLR) in cellular extracts in part through transcriptional upregulation. Although PBA induced LDLR gene expression, it did not translate into more LDLR proteins. PBA regulated cellular lipid homeostasis through the induction of CPT1A and INSIG2 expression via an epigenetic mechanism involving the acetylation of histone H3, histone H4, and CBP-p300 at the CPT1A and INSIG2 promoters.
Dorsolateral Prefrontal Cortex Activity during a Brain Training Game Predicts Cognitive Improvements after Four Weeks’ Brain Training Game Intervention: Evidence from a Randomized Controlled Trial
Authors: Nouchi Rui , Kawata Natasha Yuriko dos Santos , Saito Toshiki , Himmelmeier Robin Maximilian , Nakamura Ryo , Nouchi Haruka , Kawashima Ryuta
Published: August 2020

Background: Recent studies have demonstrated that brain activities using NIRS (near-infrared spectroscopy) at baseline during cognitive tasks (e.g., N-back task) can predict the cognitive benefits of a cognitive training. In this study, we investigated whether brain activities during brain training game (BT) at baseline would predict benefits to cognitive functions after the intervention period. Methods: In a four-week double-blinded randomized control trial (RCT) 72 young adults were randomly assigned to one of the two groups: participants in the BT group played specific game, called the Brain Age. Participants in an active control group (ACT) played the puzzle game Tetris. We measured brain activity during the training games using two channel NIRS before the intervention period. Cognitive functions were tested before and after the four-week intervention period. Results: The BT showed significant improvements in inhibition, processing speed, and working memory performance compared to ACT. The left and right DLPFC (dorsolateral prefrontal cortex) brain activities during the BT at baseline were associated with improvements in inhibition and processing speed. Discussion: This randomized control trial first provides scientific evidence that DLPFC activities during BT at baseline can predict cognitive improvements after a four-week intervention period.
Comparative Study of Organoids from Patient-Derived Normal and Tumor Colon and Rectal Tissue
Authors: Costales-Carrera Alba , Fernández-Barral Asunción , Bustamante-Madrid Pilar , Domínguez Orlando , Guerra-Pastrián Laura , Cantero Ramón , del Peso Luis , Burgos Aurora , Barbáchano Antonio , Muñoz Alberto
Published: August 2020

Colon and rectal tumors, often referred to as colorectal cancer, show different gene expression patterns in studies that analyze whole tissue biopsies containing a mix of tumor and non-tumor cells. To better characterize colon and rectal tumors, we investigated the gene expression profile of organoids generated from endoscopic biopsies of rectal tumors and adjacent normal colon and rectum mucosa from therapy-naive rectal cancer patients. We also studied the effect of vitamin D on these organoid types. Gene profiling was performed by RNA-sequencing. Organoids from a normal colon and rectum had a shared gene expression profile that profoundly differed from that of rectal tumor organoids. We identified a group of genes of the biosynthetic machinery as rectal tumor organoid-specific, including those encoding the RNA polymerase II subunits POLR2H and POLR2J. The active vitamin D metabolite 1α,25-dihydroxyvitamin D3/calcitriol upregulated stemness-related genes (LGR5, LRIG1, SMOC2, and MSI1) in normal rectum organoids, while it downregulated differentiation marker genes (TFF2 and MUC2). Normal colon and rectum organoids share similar gene expression patterns and respond similarly to calcitriol. Rectal tumor organoids display distinct and heterogeneous gene expression profiles, with differences with respect to those of colon tumor organoids, and respond differently to calcitriol than normal rectum organoids.
Retinoblastoma: Etiology, Modeling, and Treatment
Authors: Kaewkhaw Rossukon , Rojanaporn Duangnate
Published: August 2020

Retinoblastoma is a retinal cancer that is initiated in response to biallelic loss of RB1 in almost all cases, together with other genetic/epigenetic changes culminating in the development of cancer. RB1 deficiency makes the retinoblastoma cell-of-origin extremely susceptible to cancerous transformation, and the tumor cell-of-origin appears to depend on the developmental stage and species. These are important to establish reliable preclinical models to study the disease and develop therapies. Although retinoblastoma is the most curable pediatric cancer with a high survival rate, advanced tumors limit globe salvage and are often associated with high-risk histopathological features predictive of dissemination. The advent of chemotherapy has improved treatment outcomes, which is effective for globe preservation with new routes of targeted drug delivery. However, molecularly targeted therapeutics with more effectiveness and less toxicity are needed. Here, we review the current knowledge concerning retinoblastoma genesis with particular attention to the genomic and transcriptomic landscapes with correlations to clinicopathological characteristics, as well as the retinoblastoma cell-of-origin and current disease models. We further discuss current treatments, clinicopathological correlations, which assist in guiding treatment and may facilitate globe preservation, and finally we discuss targeted therapeutics for future treatments.
Systematic Analysis of Aberrant Biochemical Networks and Potential Drug Vulnerabilities Induced by Tumor Suppressor Loss in Malignant Pleural Mesothelioma
Authors: Yang Haitang , Xu Duo , Yang Zhang , Yao Feng , Zhao Heng , Schmid Ralph A. , Peng Ren-Wang
Published: August 2020

Background: Malignant pleural mesothelioma (MPM) is driven by the inactivation of tumor suppressor genes (TSGs). An unmet need in the field is the translation of the genomic landscape into effective TSG-specific therapies. Methods: We correlated genomes against transcriptomes of patients’ MPM tumors, by weighted gene co-expression network analysis (WGCNA). The identified aberrant biochemical networks and potential drug targets induced by tumor suppressor loss were validated by integrative data analysis and functional interrogation. Results: CDKN2A/2B loss activates G2/M checkpoint and PI3K/AKT, prioritizing a co-targeting strategy for CDKN2A/2B-null MPM. CDKN2A deficiency significantly co-occurs with deletions of anti-viral type I interferon (IFN-I) genes and BAP1 mutations, that enriches the IFN-I signature, stratifying a unique subset, with deficient IFN-I, but proficient BAP1 for oncolytic viral immunotherapies. Aberrant p53 attenuates differentiation and SETD2 loss acquires the dependency on EGFRs, highlighting the potential of differentiation therapy and pan-EGFR inhibitors for these subpopulations, respectively. LATS2 deficiency is linked with dysregulated immunoregulation, suggesting a rationale for immune checkpoint blockade. Finally, multiple lines of evidence support Dasatinib as a promising therapeutic for LATS2-mutant MPM. Conclusions: Systematic identification of abnormal cellular processes and potential drug vulnerabilities specified by TSG alterations provide a framework for precision oncology in MPM.
Trabectedin and Lurbinectedin Extend Survival of Mice Bearing C26 Colon Adenocarcinoma, without Affecting Tumor Growth or Cachexia
Authors: Aquila Giorgio , Re Cecconi Andrea David , Forti Mara , Frapolli Roberta , Bello Ezia , Novelli Deborah , Russo Ilaria , Licandro Simonetta Andrea , Staszewsky Lidia , Martinelli Giulia Benedetta , Talamini Laura , Pasetto Laura , Resovi Andrea , Giavazzi Raffaella , Scanziani Eugenio , Careccia Giorgia , Vénéreau Emilie , Masson Serge , Latini Roberto , D'Incalci Maurizio , Piccirillo Rosanna
Published: August 2020

Trabectedin (ET743) and lurbinectedin (PM01183) limit the production of inflammatory cytokines that are elevated during cancer cachexia. Mice carrying C26 colon adenocarcinoma display cachexia (i.e., premature death and body wasting with muscle, fat and cardiac tissue depletion), high levels of inflammatory cytokines and subsequent splenomegaly. We tested whether such drugs protected these mice from cachexia. Ten-week-old mice were inoculated with C26 cells and three days later randomized to receive intravenously vehicle or 0.05 mg/kg ET743 or 0.07 mg/kg PM01183, three times a week for three weeks. ET743 or PM01183 extended the lifespan of C26-mice by 30% or 85%, respectively, without affecting tumor growth or food intake. Within 13 days from C26 implant, both drugs did not protect fat, muscle and heart from cachexia. Since PM01183 extended the animal survival more than ET743, we analyzed PM01183 further. In tibialis anterior of C26-mice, but not in atrophying myotubes, PM01183 restrained the NF-κB/PAX7/myogenin axis, possibly reducing the pro-inflammatory milieu, and failed to limit the C/EBPβ/atrogin-1 axis. Inflammation-mediated splenomegaly of C26-mice was inhibited by PM01183 for as long as the treatment lasted, without reducing IL-6, M-CSF or IL-1β in plasma. ET743 and PM01183 extend the survival of C26-bearing mice unchanging tumor growth or cachexia but possibly restrain muscle-related inflammation and C26-induced splenomegaly.
Endocrine Adverse Events of Nivolumab in Non-Small Cell Lung Cancer Patients—Literature Review
Authors: Dudzińska Marta , Szczyrek Michał , Wojas-Krawczyk Kamila , Świrska Joanna , Chmielewska Izabela , Zwolak Agnieszka
Published: August 2020

In recent years, we have observed significant progress in cancer treatment associated with the development of immunotherapy. A programmed cell death 1 molecule (PD-1) on the surface of T lymphocytes may be stimulated via a specific PD-ligand 1 (PD-L1), which inhibits lymphocyte activation and leads to apoptosis. Some malignant cells are characterized by high PD-L1 expression. Nivolumab, an anti-PD-1 antibody, blocks the interaction between PD-1 and its ligands and inhibits the signaling pathway by preventing the tumor-derived PD-L1 from blocking T lymphocytes. In patients with non-small cell lung cancer (NSCLC), it is used either in monotherapy or in combination with other drugs. Immunotherapy is associated with the possibility of immune-related adverse effects (irAE) including endocrinopathies (3–23%). Thyroid disorders are the most common, with severity rarely exceeding grade 2. Hypophysitis, adrenal insufficiency and diabetes are possible complications which require immediate treatment. Individuals with autoimmune diseases diagnosed prior to immunotherapy are at risk of its exacerbation. In the management of patients receiving immunotherapy, evaluation of history of autoimmune diseases, awareness and early diagnosis of irAE are crucial and may affect treatment outcomes.
Understanding the Effect of Multiple Domain Deletion in DNA Polymerase I from Geobacillus Sp. Strain SK72
Authors: Hadrawi Waqiyuddin Hilmi , Norazman Anas , Mohd Shariff Fairolniza , Mohamad Ali Mohd Shukuri , Raja Abd Rahman Raja Noor Zaliha
Published: August 2020

The molecular structure of DNA polymerase I or family A polymerases is made up of three major domains that consist of a single polymerase domain with two extra exonuclease domains. When the N-terminal was deleted, the enzyme was still able to perform basic polymerase activity with additional traits that used isothermal amplification. However, the 3′-5′ exonuclease domain that carries a proofreading activity was disabled. Yet, the structure remained attached to the 5′-3′ polymerization domain without affecting its ability. The purpose of this non-functional domain still remains scarce. It either gives negative effects or provides structural support to the DNA polymerase. Here, we compared the effect of deleting each domain against the polymerase activity. The recombinant wild type and its variants were successfully purified and characterized. Interestingly, SK72-Exo (a large fragment excluding the 5′-3′ exonuclease domain) exhibited better catalytic activity than the native SK72 (with all three domains) at similar optimum temperature and pH profile, and it showed longer stability at 70 °C. Meanwhile, SK72-Exo2 (polymerization domain without both the 5′-3′ and 3′-5′ exonuclease domain) displayed the lowest activity with an optimum at 40 °C and favored a more neutral environment. It was also the least stable among the variants, with almost no activity at 50 °C for the first 10 min. In conclusion, cutting both exonuclease domains in DNA polymerase I has a detrimental effect on the polymerization activity and structural stability.
Developing Low-Cost, High Performance, Robust and Sustainable Perovskite Electrocatalytic Materials in the Electrochemical Sensors and Energy Sectors: “An Overview”
Authors: Chen Tse-Wei , Ramachandran Rasu , Chen Shen-Ming , Kavitha Narayanasamy , Dinakaran Kannaiyan , Kannan Ramanjam , Anushya Ganesan , Bhuvana Nagulan , Jeyapragasam Tharini , Mariyappan Vinitha , Divya Rani Selvarajan , Chitra Selvam
Published: August 2020

Since its discovery in 1839, research on the synthesis and application of perovskite materials has multiplied largely due to their suitability to be used in the fields of nanotechnology, chemistry and material science. Appropriate changes in composition or addition of other elements or blending with polymers may result in new hybrid and/or composite perovskite materials that will be applied in advanced fields. In this review, we have recapitulated the recent progress on perovskite nanomaterial in solar cell, battery, fuel cell and supercapacitor applications, and the prominence properties of perovskite materials, such as excellent electronic, physical, chemical and optical properties. We discussed in detail the synthesis and results of various perovskite hybrid nanomaterials published elsewhere. We have also discussed the results of various studies on these low dimensional composite nanomaterials in broad sectors such as electronics/optoelectronics, batteries, supercapacitors, solar cells and electrochemical sensors.
In Vivo siRNA Delivery to Immunosuppressive Liver Macrophages by α-Mannosyl-Functionalized Cationic Nanohydrogel Particles
Authors: Kaps Leonard , Leber Nadine , Klefenz Adrian , Choteschovsky Niklas , Zentel Rudolf , Nuhn Lutz , Schuppan Detlef
Published: August 2020

Macrophages are the front soldiers of the innate immune system and are vital for immune defense, tumor surveillance, and tissue homeostasis. In chronic diseases, including cancer and liver fibrosis, macrophages can be forced into an immunosuppressive and profibrotic M2 phenotype. M2-type macrophages overexpress the mannose receptor CD206. Targeting these cells via CD206 and macrophage repolarization towards an immune stimulating and antifibrotic M1 phenotype through RNA interference represents an appealing therapeutic approach. We designed nanohydrogel particles equipped with mannose residues on the surface (ManNP) that delivered siRNA more efficiently to M2 polarized macrophages compared to their untargeted counterparts (NonNP) in vitro. The ManNP were then assessed for their in vivo targeting potential in mice with experimental liver fibrosis that is characterized by increased profibrotic (and immunosuppressive) M2-type macrophages. Double-labelled siRNA-loaded ManNP carrying two different near infrared labels for siRNA and ManNP showed good biocompatibility and robust uptake in fibrotic livers as assessed by in vivo near infrared imaging. siRNA–ManNP were highly colocalized with CD206+ M2-type macrophages on a cellular level, while untargeted NP (NonNP) showed little colocalization and were non-specifically taken up by other liver cells. ManNP did not induce hepatic inflammation or kidney dysfunction, as demonstrated by serological analysis. In conclusion, α-mannosyl-functionalized ManNP direct NP towards M2-type macrophages in diseased livers and prevent unspecific uptake in non-target cells. ManNP are promising vehicles for siRNA and other drugs for immunomodulatory treatment of liver fibrosis and liver cancer.
Targeted Therapy for Pulmonary Hypertension in Premature Infants
Authors: Nees Shannon N. , Rosenzweig Erika B. , Cohen Jennifer L. , Valencia Villeda Gerson A. , Krishnan Usha S.
Published: August 2020

Pulmonary hypertension (PH) is common in premature infants with bronchopulmonary dysplasia (BPD) and is associated with significant mortality. Despite expert consensus suggesting the use of targeted therapies such as phosphodiesterase inhibitors, endothelin receptor antagonists, and prostanoids, there is little data on safety and outcomes in infants with BPD-associated PH (BPD-PH) treated with these medications. We sought to describe the pharmacologic management of BPD-PH and to report outcomes at our institution. Premature infants with BPD-PH born between 2005 and 2016 were included. Follow-up data were obtained through January 2020. A total of 101 patients (61 male, 40 female) were included. Of these, 99 (98.0%) patients were treated with sildenafil, 13 (12.9%) with bosentan, 35 (34.7%) with inhaled iloprost, 12 (11.9%) with intravenous epoprostenol, and nine (8.9%) with subcutaneous treprostinil. A total of 33 (32.7%) patients died during the study period and 10 (9.9%) were secondary to severe to pulmonary hypertension. Of the surviving patients, 57 (83.8%) had follow-up data at a median of 5.1 (range 0.38–12.65) years and 44 (77.2%) were weaned off PH medications at a median 2.0 (range 0–8) years. Mortality for BPD-PH remains high mostly due to co-morbid conditions. However, for those patients that survive to discharge, PH therapies can frequently be discontinued in the first few years of life.
Structure and Properties of AlCrN Coatings Deposited Using Cathodic Arc Evaporation
Authors: Warcholinski Bogdan , Gilewicz Adam , Myslinski Piotr , Dobruchowska Ewa , Murzynski Dawid
Published: August 2020

Al–Cr–N coatings were formed at various nitrogen pressures, substrate bias voltages and substrate temperatures using cathodic arc evaporation. The relationship between technological parameters and properties of the coatings was investigated. The phase and chemical composition of the coatings, roughness, hardness, adhesion and thermal stability were analyzed by X-ray diffraction (XRD), scanning electron microscope (SEM), Energy-dispersive X-ray spectroscopy (EDX), micro-indenter, Rockwell, scratch tester and thermomechanical methods. The corrosion resistance of selected coatings was also investigated. XRD analysis indicates that the coatings crystallize in a cubic structure and show preferential orientation (200) CrN. With the increase of nitrogen pressure, the preferential orientation changes to (111). EDX analysis shows that as nitrogen pressure increases, the Al/(Al + Cr) rate decreases. Microscopic observations indicate that the number of macroparticles reduces as nitrogen pressure increases. As a result, the surface roughness parameter Ra of the coatings decreases. The effects of deposition temperature, nitrogen pressure and substrate bias voltage on the mechanical and tribological properties of the coatings were investigated. It was found that the above parameters influence the mechanical properties in different ways. The hardness and adhesion of coatings formed at higher temperatures was lower. Coatings formed under a higher nitrogen pressure or substrate bias voltage were characterized by higher hardness and better wear resistance.
Design of Liver Functional Reserve Estimation Technique Based on Optical Densitometry
Authors: Savchenko Ekaterina , Kolokolnikov Ilya , Velichko Elena , Osovskikh Victor , Kiseleva Lyubov , Musakulova Zhyldyz
Published: August 2020

This work is aimed at creating a modified invasive technique for assessing the liver’s functional reserves. A study of the degree of hepatodepression is carried out by measuring the plasma elimination of indocyanine green using the method of optical densitometry. This paper presents test results for an aqueous solution and an albumin solution, as well as the results of measurements of plasma elimination of indocyanine green for patients with liver disease. Perfecting the proposed method will make an important scientific contribution to modern diagnostic medicine. Diagnosing the stages in the progression of the disease and its developing complications can make it possible to rapidly correct the patient’s treatment algorithm, achieving positive outcomes in medical practice.
Context-Specific Challenges, Opportunities, and Ethics of Drones for Healthcare Delivery in the Eyes of Program Managers and Field Staff: A Multi-Site Qualitative Study
Authors: Jeyabalan Vyshnave , Nouvet Elysée , Meier Patrick , Donelle Lorie
Published: August 2020

Unmanned aerial vehicles (UAVs), also known as drones, have significant potential in the healthcare field. Ethical and practical concerns, challenges, and complexities of using drones for specific and diverse healthcare purposes have been minimally explored to date. This paper aims to document and advance awareness of diverse context-specific concerns, challenges, and complexities encountered by individuals working on the front lines of drones for health. It draws on original qualitative research and data from semi-structured interviews (N = 16) with drones for health program managers and field staff in nine countries. Directed thematic analysis was used to analyze interviews and identify key ethical and practical concerns, challenges, and complexities experienced by participants in their work with drones for health projects. While some concerns, challenges, and complexities described by study participants were more technical in nature, for example, those related to drone technology and approval processes, the majority were not. The bulk of context-specific concerns and challenges identified by participants, we propose, could be mitigated through community engagement initiatives.
Unified System Network Architecture: Flexible and Area-Efficient NoC Architecture with Multiple Ports and Cores
Authors: Bui Phan-Duy , Lee Chanho
Published: August 2020

In recent years, as semiconductor manufacturing processes have been steadily scaled down, the transistor count fabricated on a single silicon die can reach up to a billion units. Therefore, current multiprocessor system-on-chips (MPSoCs) can include up to hundreds or even thousands of cores and additional accelerators for high-performance systems. Network-on-chips (NoCs) have become an attractive solution for interconnects, which are critical components of MPSoCs in terms of system performance. In this study, a highly flexible and area-efficient NoC architecture, namely the unified system network architecture (USNA), which can be tailored for various topologies, is proposed. The USNA provides high flexibility in port placements with varying numbers of local cores and router linkers. It also supports quality of service operations for both the router and linker. The network performance (e.g., average latency and saturated throughput) and implementation cost of the USNA, using various network configurations for the same number of local cores under uniform random traffic conditions, were investigated in this study. According to the simulation results, the performance of the USNA is better or similar to other NoCs, with a significantly smaller area and lower power consumption.
Lexicon-based Sentiment Analysis Using the Particle Swarm Optimization
Authors: Machová Kristína , Mikula Martin , Gao Xiaoying , Mach Marian
Published: August 2020

This work belongs to the field of sentiment analysis; in particular, to opinion and emotion classification using a lexicon-based approach. It solves several problems related to increasing the effectiveness of opinion classification. The first problem is related to lexicon labelling. Human labelling in the field of emotions is often too subjective and ambiguous, and so the possibility of replacement by automatic labelling is examined. This paper offers experimental results using a nature-inspired algorithm—particle swarm optimization—for labelling. This optimization method repeatedly labels all words in a lexicon and evaluates the effectiveness of opinion classification using the lexicon until the optimal labels for words in the lexicon are found. The second problem is that the opinion classification of texts which do not contain words from the lexicon cannot be successfully done using the lexicon-based approach. Therefore, an auxiliary approach, based on a machine learning method, is integrated into the method. This hybrid approach is able to classify more than 99% of texts and achieves better results than the original lexicon-based approach. The final hybrid model can be used for emotion analysis in human–robot interactions.
An Energy-Efficient Integration of a Digital Modulator and a Class-D Amplifier
Authors: Zia Muhammad Yousuf Irfan , Tierno Raúl , Luque-Nieto Miguel-Ángel , Otero Pablo
Published: August 2020

Energy consumption is always a key feature in devices powered by electric accumulators. The power amplifier is the most energy-demanding module in mobile devices, portable appliances, static transceivers, and even nodes used in underwater acoustic networks. These devices incorporate a modulator, typically a pulse-width modulation (PWM) and a class-D power amplifier, for higher efficiency. We propose a technique to integrate the modulator of a transmitter and PW-modulator of a class-D amplifier to improve the overall efficiency of the system. This integrated set operates as an up-converter, phase modulator (PM), and binary phase-shift keying (BPSK) modulator under certain conditions. The theoretical concept is verified using Matlab and a model is designed and simulated in Simulink. For validation purposes, an electronic circuit is built and tested using Multisim. The results obtained by simulations and circuit implementation show that the proposed integrated system is an energy-efficient and cost-effective solution compared to conventional techniques.
Predicting Car Availability in Free Floating Car Sharing Systems: Leveraging Machine Learning in Challenging Contexts
Authors: Daraio Elena , Cagliero Luca , Chiusano Silvia , Garza Paolo , Giordano Danilo
Published: August 2020

Free-Floating Car Sharing (FFCS) services are currently available in tens of cities and countries spread all over the worlds. Depending on citizens’ habits, service policies, and road conditions, car usage profiles are rather variable and often hardly predictable. Even within the same city, different usage trends emerge in different districts and in various time slots and weekdays. Therefore, modeling car availability in FFCS systems is particularly challenging. For these reasons, the research community has started to investigate the applicability of Machine Learning models to analyze FFCS usage data. This paper addresses the problem of predicting the short-term level of availability of the FFCS service in the short term. Specifically, it investigates the applicability of Machine Learning models to forecast the number of available car within a restricted urban area. It seeks the spatial and temporal contexts in which nonlinear ML models, trained on past usage data, are necessary to accurately predict car availability. Leveraging ML has shown to be particularly effective while considering highly dynamic urban contexts, where FFCS service usage is likely to suddenly and unexpectedly change. To tailor predictive models to the real FFCS data, we study also the influence of ML algorithm, prediction horizon, and characteristics of the neighborhood of the target area. The empirical outcomes allow us to provide system managers with practical guidelines to setup and tune ML models.
A Review and Comparison of Solid, Multi-Strands and Litz Style PCB Winding
Authors: Nguyen Minh Huy , Fortin Blanchette Handy
Published: August 2020

At high frequency, AC resistance of a printed circuit board (PCB) winding becomes important and accounts for a large proportion of planar transformer losses. The winding is then influenced by both skin and proximity phenomenon, which makes the current distribution uneven resulting in an increased resistance. The study of improving AC resistance of a PCB winding has been tackled by many researchers. However, the lack of an overview and comparison among improvements has made it difficult to apply those methods to a specific winding. To overcome the above limitations, this paper investigates the pros and cons of three popular AC resistance optimizing methods: optimizing track width of a solid PCB winding, using multi-strands and using Litz style PCB winding. To verify the theoretical analysis, a total of 12 PCBs are simulated by finite element (FEM) and tested in the laboratory. Five criteria are analyzed, including skin resistance, proximity resistance, AC to DC ratio, total AC resistance and complexity are taken into consideration. The results of this study show that optimizing track width method has a significant improvement on AC resistance while the use of Litz PCB is effective for applications that need stable AC resistance in a wide frequency range. The use of parallel strands winding should be carefully considered as there is not significant benefit in both reducing the AC resistance and AC to DC ratio.
Characterization of the Key Aroma Constituents in Fry Breads by Means of the Sensomics Concept
Authors: Lasekan Ola , Dabaj Fatma
Published: August 2020

The key aroma constituents in the volatile fractions isolated FROM two differently processed fry breads by solvent-assisted flavor evaporation were characterized by an aroma extract dilution analysis (AEDA). Twenty-two compounds were identified with flavor dilution (FD) factor ranges of 2–516. Among them, 13 compounds (FD ≥ 16) were quantified by stable isotope dilution assays and analyzed by odor activity values (OAVs). Of these, 11 compounds had OAVs ≥ 1, and the highest concentrations were determined for δ-decalactone and 2,3-butanedione. Two recombination models of the fry breads showed similarity to the corresponding fry breads. Omission tests confirmed that aroma-active constituents, such as δ-decalactone (oily/peach), 2-acetyl-1-pyrroline (roasty/popcorn-like), 3-methylbutanal (malty), methional (baked potato-like), 2,3-butanedione (buttery), phenyl acetaldehyde (flowery), (E,E)-2,4-decadienal (deep-fried), butanoic acid, and 3-methylbutanoic acid, were the key aroma constituents of fry bread. In addition, 3-methoxy-4-vinylphenol (smoky) and 4-hydroxy-2,5-dimethyl-3(2H)-furanone were also identified as important aroma constituents of fry bread.
Impact of Extreme Weather Events on Aboveground Net Primary Productivity and Sheep Production in the Magellan Region, Southernmost Chilean Patagonia
Authors: Soto-Rogel Pamela , Aravena Juan-Carlos , Meier Wolfgang Jens-Henrik , Gross Pamela , Pérez Claudio , González-Reyes Álvaro , Griessinger Jussi
Published: August 2020

Spatio-temporal patterns of climatic variability have effects on the environmental conditions of a given land territory and consequently determine the evolution of its productive activities. One of the most direct ways to evaluate this relationship is to measure the condition of the vegetation cover and land-use information. In southernmost South America there is a limited number of long-term studies on these matters, an incomplete network of weather stations and almost no database on ecosystems productivity. In the present work, we characterized the climate variability of the Magellan Region, southernmost Chilean Patagonia, for the last 34 years, studying key variables associated with one of its main economic sectors, sheep production, and evaluating the effect of extreme weather events on ecosystem productivity and sheep production. Our results show a marked multi-decadal character of the climatic variables, with a trend to more arid conditions for the last 8 years, together with an increase in the frequency of extreme weather events. Significant percentages of aboveground net primary productivity (ANPP) variance is explained by high precipitation, mesic temperatures, and low evapotranspiration. These conditions are, however, spatially distributed in the transition zone between deciduous forests and steppe and do not represent a general pattern for the entire region. Strong precipitation and wind velocity negatively affect lamb survival, while temperature and ANPP are positively correlated. The impact of extreme weather events on ANP and sheep production (SP) was in most of the cases significantly negative, with the exception of maximum temperature that correlated with an increase of ANPP, and droughts that showed a non-significant negative trend in ANPP. The examination of these relationships is urgent under the current scenario of climate change with the acceleration of the environmental trends here detected.
Laboratory and Physical Prototype Tests for the Investigation of Hydraulic Hysteresis of Pyroclastic Soils
Authors: Pirone Marianna , Reder Alfredo , Rianna Guido , Pagano Luca , Nicotera Marco Valerio , Urciuoli Gianfranco
Published: August 2020

Proper soil water retention curves (SWRCs) are necessary for a fair analysis of groundwater flow in unsaturated slopes. The question is whether hydraulic parameters operating in situ can be reliably determined from laboratory tests or physical prototype models in order to interpret and predict soil water distributions in the field. In this paper, some results obtained by tests at different scales (testing on laboratory specimens and a physical prototype) are presented to explore the hydraulic behavior of pyroclastic soils. A theoretical interpretation of the observed behavior in the laboratory and using a physical prototype is proposed by adopting the hysteretic model of Lenhard and Parker. For each tested soil, the main hysteretic loop determined by interpreting experimental tests (at laboratory and prototype scales) overlaps with paths detected by coupling the field measurements of matric suction and water content collected at the site at the same depth. From these results, the physical prototype (medium scale) and the soil specimen (small scale) seem to be acceptable for determinations of SWRC, provided that the air entrapment value is well known.
Correlation between Lung Function and Functional Movement in Healthy Adults
Authors: Kim Hyun-Seung , Cho Sung-Hyoun
Published: August 2020

It is difficult to determine whether lung function improves by simple abdominal strengthening exercises, and studies on the correlation of lung function and functional movement are insufficient. Therefore; we aimed to identify the correlation between lung function and functional movement. We recruited 204 healthy adults and measured their forced vital capacity; forced expiratory volume in 1 s, maximum voluntary ventilation, maximum expiratory pressure (MEP), and maximum inspiratory pressure (MIP). We also extracted data obtained during functional movements. Differences between lung function and functional movement by gender were determined using independent sample t-tests, while the relationship between lung function and functional movement variables were analyzed using Pearson′s correlation coefficient. Significant gender-based differences between lung function and functional movement, a negative correlation between MIP and inline lunge, and a significantly positive correlation between MIP and trunk stability push-up occurred in males. In females, a positive correlation occurred between MEP and hurdle step, MIP and deep squat, and MIP and hurdle step. Aspects of lung function (MEP and MIP) showed correlations with functional movements. The results showed that lung function and functional movement were correlated, confirming that there is a relationship between lung function and functional movement in healthy adults.