bluuebunny/crossref_metadata_2025 huggingface dataset:
Exploring the Interconnection between Metabolic Dysfunction and Gut Microbiome Dysbiosis in Osteoarthritis: A Narrative Review
Authors: Li Hui , Wang Jihan , Hao Linjie , Huang Guilin
Published: September 2024

Osteoarthritis (OA) is a prevalent joint disorder and the most common form of arthritis, affecting approximately 500 million people worldwide, or about 7% of the global population. Its pathogenesis involves a complex interplay between metabolic dysfunction and gut microbiome (GM) alterations. This review explores the relationship between metabolic disorders—such as obesity, diabetes, and dyslipidemia—and OA, highlighting their shared risk factors, including aging, sedentary lifestyle, and dietary habits. We further explore the role of GM dysbiosis in OA, elucidating how systemic inflammation, oxidative stress, and immune dysregulation driven by metabolic dysfunction and altered microbial metabolites contribute to OA progression. Additionally, the concept of “leaky gut syndrome” is discussed, illustrating how compromised gut barrier function exacerbates systemic and local joint inflammation. Therapeutic strategies targeting metabolic dysfunction and GM composition, including lifestyle interventions, pharmacological and non-pharmacological factors, and microbiota-targeted therapies, are reviewed for their potential to mitigate OA progression. Future research directions emphasize the importance of identifying novel biomarkers for OA risk and treatment response, adopting personalized treatment approaches, and integrating multiomics data to enhance our understanding of the metabolic–GM–OA connection and advance precision medicine in OA management.
A Novel AKT1, ERBB2, ESR1, KRAS, PIK3CA, and TP53 NGS Assay: A Non-Invasive Tool to Monitor Resistance Mechanisms to Hormonal Therapy and CDK4/6 Inhibitors
Authors: Virga Alessandra , Gianni Caterina , Palleschi Michela , Angeli Davide , Merloni Filippo , Maltoni Roberta , Ulivi Paola , Martinelli Giovanni , De Giorgi Ugo , Bravaccini Sara
Published: September 2024

Background: Patients with hormone receptor-positive (HR+)/HER2- metastatic breast cancer (mBC) generally receive hormonal therapy (HT) combined with CDK4/6 inhibitors (CDK4/6i). Despite this treatment, resistance mechanisms to CDK4/6i emerge and the majority of these patients experience disease progression (PD). This highlight the necessity to uncover the resistance mechanism to CDK4/6i through the identification of specific biomarkers. The primary objective is to assess the accuracy and feasibility of a novel multi-gene target panel NGS assay on circulating tumor DNA (ctDNA) to detect molecular alterations of AKT1, ERBB2, ESR1, KRAS, PIK3CA, and TP53 genes in women with BC undergoing HT plus CDK4/6i treatment. Secondarily, the study aims to explore the relationship between genomic profiling and clinical outcomes. Materials and Methods: Plasma samples were collected from 16 patients diagnosed with advanced/locally advanced HR+/HER2- BC at 2 time points: T0 (baseline) and at T1 (3 months after CDK4/6i treatment). Starting from 2 mL of plasma, ctDNA was isolated and libraries were set up using the Plasma-SeqSensei (PQS)® Breast Cancer IVD Kit, sequenced on Nextseq 550 and analyzed using the Plasma-SeqSensei™ IVD Software®. Results: Among the five patients who presented PD, three had PIK3CA mutations and, of these, two showed a higher mutant allele frequency (MAF) at T1. In three patients with stable disease and in eight patients with partial response, the MAF of the detected alterations decreased dramatically or disappeared during CDK4/6i treatment. Conclusions: Based on our findings, the liquid biopsy analysis using the PQS panel seems to be both feasible and accurate, demonstrating a strong sensitivity in detecting mutations. This exploratory analysis of the clinical outcome associated to the mutational status of patients highlights the potential of molecular analysis on liquid biopsy for disease monitoring, although further validation with a larger patient cohort is necessary to confirm these preliminary observations.
TH1 Cell Frequency and Neutrophil-to-Lymphocyte Ratio in Hemodialysis: Potential Contributions to Patient Monitoring
Authors: Rodrigues Barreto Inês , Monteiro Andreia , Rocha Ernesto Fernandes , Santos Catarina Reis , Fonseca Ana Mafalda
Published: September 2024

Introduction: Patients undergoing hemodialysis (HD) often exhibit an impaired cellular immune response, which may contribute to an increased susceptibility to infections and other complications. Th1 cells, a subset of T-helper cells, play a crucial role in cellular immunity. However, the modulation of Th1 cells by HD treatment remains unclear. Objective: This study aims to investigate the levels of circulating T cells, especially Th1 cells, and the neutrophil-to-lymphocyte ratio (NLR) in HD patients. Methods: We recruited 26 HD patients and 10 healthy volunteers. Demographical data were collected, and peripheral blood samples were analyzed. Absolute blood cell counts were determined, and T-cell populations were identified using flow cytometry. Th1 cells were defined as IFN-γ-producing CD4+ T cells after in vitro activation, and NLR was calculated through the ratio between the neutrophil and lymphocyte counts measured in peripheral blood. Results: We have observed a significant decrease in Th1 subpopulation frequency in HD patients, as well as significant correlations between immunological and demographic parameters, among which are the NLR values and the absolute values of T-cell subsets. Conclusions: These results seem to clarify the role of Th1 cells in modulating the immune responses of hemodialysis-treated patients, potentially considering its frequency as an indicator for CKD development.
How Does Cancer Occur? How Should It Be Treated? Treatment from the Perspective of Alkalization Therapy Based on Science-Based Medicine
Authors: Hamaguchi Reo , Isowa Masahide , Narui Ryoko , Morikawa Hiromasa , Okamoto Toshihiro , Wada Hiromi
Published: September 2024

This review article investigates the relationship between mitochondrial dysfunction and cancer progression, emphasizing the metabolic shifts that promote tumor growth. Mitochondria are crucial for cellular energy production, but they also play a significant role in cancer progression by promoting glycolysis even under oxygen-rich conditions, a phenomenon known as the Warburg effect. This metabolic reprogramming enables cancer cells to maintain an alkaline internal pH and an acidic external environment, which are critical for their proliferation and survival in hypoxic conditions. The article also explores the acidic tumor microenvironment (TME), a consequence of intensive glycolytic activity and proton production by cancer cells. This acidic milieu enhances the invasiveness and metastatic potential of cancer cells and contributes to increased resistance to chemotherapy. Alkalization therapy, which involves neutralizing this acidity through dietary modifications and the administration of alkalizing agents such as sodium bicarbonate, is highlighted as an effective strategy to counteract these adverse conditions and impede cancer progression. Integrating insights from science-based medicine, the review evaluates the effectiveness of alkalization therapy across various cancer types through clinical assessments. Science-based medicine, which utilizes inductive reasoning from observed clinical outcomes, lends support to the hypothesis of metabolic reprogramming in cancer treatment. By addressing both metabolic and environmental disruptions, this review suggests that considering cancer as primarily a metabolic disorder could lead to more targeted and effective treatment strategies, potentially improving outcomes for patients with advanced-stage cancers.
Numerical Study of the Effect of the Port Angle of the Superior Vena Cava Supplying Cannula on Hemodynamics in the Right Atrium in VV-ECMO
Authors: Ma Xinrui , Xu Kaihang , Gao Bin
Published: September 2024

Objective: To elucidate the pattern of the influence of the port angle of the superior vena cava supplying cannula (SVCS) on hemodynamics within the right atrium in VV-ECMO. Methods: A three-dimensional model of the right atrium was established based on CT images of a real patient. The 3D models of the SVCS and inferior vena cava draining cannula (IVCD) were established based on the Edwards 18Fr and Medos 22Fr real intubation models, respectively. Based on these models, three-dimensional models of the SVCS ports with bending angles of −90°, −60°, −30°, 0°, 30°, 60°, and 90° in the plane formed by the centerline of the SVCS and the center point of the tricuspid valve (TV) were established. Transient-state computational fluid dynamics (CFD) was performed to clarify the right atrium blood flow pattern and hemodynamic states at different SVCS port orientation angles. The velocity clouds, wall pressure, wall shear stress (WSS), relative residence time (RRT), and recirculation fraction (RF) were calculated to assess hemodynamic changes in the right atrium at different angles of the port of the SVCS. Results: As the angle of the port of the superior chamber cannula changed, the location of the high-velocity blood impingement from the SVCS changed, and the pattern of blood flow within the right atrium was dramatically altered. The results for the maximum right atrial wall pressure were 13,472 pa, 13,424 pa, 10,915 pa, 7680.2 pa, 5890.3 pa, 5597.6 pa, and 7883.5 pa (−90° vs. −60° vs. −30° vs. 0° vs. 30° vs. 60° vs. 90°), and the results for the mean right atrial wall pressure were 6788.9 pa, 8615.1 pa, 8684.9 pa, 6717.2 pa, 5429.2 pa, 5455.6 pa, and 7117.8 pa ( −90° vs. −60° vs. −30° vs. 0° vs. 30° vs. 60° vs. 90°). The results of the maximum right atrial wall WSS in the seven cases were 63.572 pa, 55.839 pa, 31.705 pa, 39.531 pa, 40.11 pa, 28.474 pa, and 35.424 (−90° vs. −60° vs. −30° vs. 0° vs. 30° vs. 60° vs. 90°), respectively, and the results of the mean right atrial wall WSS results were 3.8589 pa, 3.6706 pa, 3.3013 pa, 3.2487 pa, 2.3995 pa, 1.3304 pa, and 2.0747 pa (−90° vs. −60° vs. −30° vs. 0° vs. 30° vs. 60° vs. 90°), respectively. The results for the area percentage of high RRT in the seven cases were 3.44%, 2.23%, 4.24%, 1.83%, 3.69%, 7.73%, and 3.68% (−90° vs. −60° vs. −30° vs. 0° vs. 30° vs. 60° vs. 90°), and the results for the RF were 21.57%, 23.24%, 19.78%, 12.57%, 10.24%, 5.07%, and 8.05% (−90° vs. −60° vs. −30° vs. 0° vs. 30° vs. 60° vs. 90°). Conclusions: The more the port of the SVCS is oriented toward the TV, the more favorable it is for reducing RF and the impingement of blood flow in the right atrial wall, but there may be an increased risk of RRT. The opposite orientation of the SVCS port to the TV is not conducive to reducing flow impingement on the right atrial wall and RF.
Effect of an In-Home Palivizumab Administration Program for Children with Medical Complexity
Authors: Emanuel Hina , Yadav Aravind , Eapan Julie C. , Caldas-Vasquez Maria , Harris Tomika S. , McBeth Katrina , Boricha Fatima , John Janice , Magana Ceballos. Ivan G. , Colasurdo Giuseppe N. , Tellez Maria E. , Reddy Tina , De Jesús-Rojas Wilfredo , Mosquera Ricardo A.
Published: September 2024

Background: In-home palivizumab administration programs (PH) have shown promise in reducing RSV-associated infections. These programs may be particularly beneficial for children with medical complexity (CMC) by limiting their exposure to healthcare-associated infections (HAIs) from non-RSV-related pathogens during transportation and visits to medical facilities. Methods: In this prospective study, 41 children with CMC less than 2 years of age were randomized by their health insurance to receive PH or in the clinic (PC) during the RSV season (October 2018–April 2019). Patients were stratified by home ventilation. The primary outcome was the total number of face-to-face encounters. Secondary outcomes were unscheduled clinic visits and hospitalizations secondary to the non-RSV LRTIs. Standard frequentist and Bayesian analyses were performed. Results: All demographic factors and strata were matched between PH (“n” = 13, mean age 22 mo. SD ± 1), and PC (“n” = 28, mean age: 18 mo. SD ± 1). There was a decrease in the number of total face-to-face encounters (adjusted for mechanical ventilation and baseline diagnosis) [(4.5 vs. 8.8), (RR: 1.8, 95% CI: 1.3–2.5, p = 0.001)], and hospitalizations [(0.3 vs. 1.25), (RR: 3.8, 95% CI: 1.3–11.3, p = 0.016)], in the PH vs PC groups. Bayesian analysis showed a 93% probability of benefit in favor of fewer face-to-face encounters in the PH group. Conclusions: This study suggests that PH administration may reduce healthcare utilization in CMC. Minimizing exposure to healthcare facilities and supporting home-based interventions are promising strategies for this population.
The Effects of Exergames on Physical Fitness, Body Composition and Enjoyment in Children: A Six-Month Intervention Study
Authors: Marsigliante Santo , My Giulia , Mazzotta Gianmarco , Muscella Antonella
Published: September 2024

Background/Objectives: Physical inactivity in children can lead to decreased physical fitness and reduced enjoyment of physical activity. This study aimed to evaluate the impact of exergaming on physical fitness, body composition and perceived enjoyment in elementary school children. Methods: Sixty-four male students (mean age 9.5 years) were randomly assigned to an exergaming group (EG, n = 32), engaging in Kinect Adventures three times a week for six months, or a control group (CG, n = 32), which continued standard physical education. Assessments were conducted pre- and post-intervention, including anthropometric measures, physical fitness tests (standing long jump, countermovement jump, sit-and-reach, 20-m sprint), aerobic fitness (20-m shuttle run), and perceived enjoyment measured with the PACES scale. Results: The EG demonstrated significant reductions in body weight, BMI, and relative fat mass compared to the CG (p < 0.01). Improvements in physical fitness were evident in EG, with increases in standing long jump distance (+12.8%; p < 0.0001), countermovement jump height (+65%, p < 0.0001), and flexibility (+75%; p < 0.0001). Aerobic fitness improved significantly in EG (+87.8m) compared to CG. Perceived enjoyment was notably higher in EG, especially at week three, compared to CG (69.3 ± 5.8 vs. 44.2 ± 11.6; p < 0.0001). Conclusions: Exergaming offers benefits for physical fitness and body composition in children, while also enhancing enjoyment. Incorporating exergames into physical education programs could be an effective strategy for addressing childhood obesity, improving physical skills, and increasing student enjoyment, encouraging long-term physical activity adherence.
Taxes, Leverage, and Profit Shifting in Banks
Authors: Joia Arthur José Cunha Bandeira de Mello , Barros Lucas Ayres Barreira de Campos , Ermel Marcelo Daniel Araujo
Published: September 2024

The goal of this research is to investigate whether taxation affects the leverage decisions of banks and if the response of leverage to tax increases depends on profit-shifting opportunities available to individual banks. This topic remains controversial since it is often believed that banking regulation is such an essential driver of leverage choices that little room is left for other considerations studied in the corporate finance literature. Using a difference-in-differences setup encompassing the period from 2006 to 2017, we exploit two exogenous income tax rate increases applicable to 225 Brazilian banks, employing novel identification strategies based on the intricacies of local taxation rules and on the distinctions between individual banks and financial conglomerates. We find stark differences in the behavior of banks around the two events, with a substantial increase in leverage following the first tax hike but no leverage response following the second. In addition, we find no evidence of heterogeneous effects based on the amount of profit-shifting opportunities available to individual banks. Regulatory concerns possibly became more relevant for leverage decisions during the period around the second tax hike because it coincided with the implementation of stricter capital requirements associated with the Basel III framework. Taken together, our results suggest that financial institutions balance considerations regarding the tax-shield benefits of debt against regulatory concerns specific to the banking industry when making capital structure choices.
The Relationship between PM2.5, Greenness, and Road Noise Exposures and Children’s Cognitive Performance in England: The Millennium Cohort Study
Authors: Garkov Sophia , Dearden Lorraine , Armstrong Ben , Milojevic Ai
Published: September 2024

Research to date suggests that air pollution may affect children’s cognitive development. This study followed 12,159 children in the Millennium Cohort Study in England for 17 years to assess the impacts of lifetime PM2.5 exposure at home and school on cognitive performance while accounting for the inter-related environmental factors of greenness and road noise. Lifetime environmental exposures were measured at home from age 9 months and at school from age 5 years. The relationships between PM2.5 and cognitive test performance at ages 3, 5, 7, 11, 14 and 17 years were investigated using multivariable linear regression models accounting for survey design and controlling for greenness, road noise, and other individual, family, school, and areal characteristics. The results suggest little evidence of observable associations between PM2.5 and cognitive performance in England, with or without adjustment for greenness and road noise, at any age in the study population. These findings also apply to greenness and road noise. This is the first study to quantify the relationship between air pollution, greenspace, noise, and children’s cognitive performance in a longitudinal cohort study in England.
Optimizing the Brewing Process, Metabolomics Analysis, and Antioxidant Activity Analysis of Complexed Hongqu Rice Wine with Kiwiberry
Authors: Wang Mengze , Liu Yuxin , Guo Xing , Ding Yuping , Liu Dejiang
Published: September 2024

Hongqu rice wine is a traditional Chinese yellow wine produced from a single ingredient. To enhance the competitiveness of the product and better adapt to market development trends, the development of a complexed Hongqu rice wine using a variety of ingredients is necessary to enhance the nutritional value of the product and diversify its flavor. This study explored production technology for the development of a complexed Hongqu rice wine using kiwiberry as the raw material. The mixed fermentation process was optimized using single-factor experiments and response surface methodology (RSM). The optimal conditions were a juice addition time of 93 h, a fermentation temperature of 31 °C, and a juice addition amount of 75 g/100 g. Under these conditions, the complexed Hongqu rice wine had an alcohol content of 8.7% vol, a total phenolic content of 0.42 mg GAE/mL, and a total flavonoid content of 0.32 mg CE/mL. In total, 27 metabolites were identified. The relative levels of 15 metabolites, including quercetin-3-glucoside and rutin, increased significantly after the adding of the kiwiberry (VIP > 1.0, p < 0.05, FC > 2). Antioxidant activity experiments showed that the Hongqu rice wine had notable antioxidant capacity and that adding the kiwiberry significantly enhanced this capacity. Additionally, the complexed Hongqu rice wine exhibited hypoglycemic and bile acid-binding properties. It achieved 78.68 ± 0.44% inhibition of α-amylase and 58.02 ± 0.50% inhibition of α-glucosidase. The binding activities with sodium glycocholate, sodium cholate, and sodium taurocholate were 40.25 ± 0.64%, 49.08 ± 1.05%, and 60.58 ± 0.80%, respectively. Consequently, a complexed Hongqu rice wine rich in quercetin-3-glucoside and rutin, with notable antioxidant activities, was developed. This wine has potential applications in functional food development.
The Influence of Injection Strategy on the Ignition Characteristics of Diesel/Ammonia Premixture
Authors: Gao Xianli , Zhou Qingxing , Jia Baofu , Cui Zechuan , Yang Hongen , Shi Song , Tian Jiangping
Published: September 2024

In this paper, based on the optical experimental platform of constant combustion and rapid compression expansion machines, the effects of spray characteristics and injection strategies of diesel in an atmosphere with ammonia on the ignition characteristics of a diesel/ammonia premixture are studied. It is found that in the spray development stage, the liquid-phase penetration distance in ammonia is greater than that in nitrogen. However, the results are opposite in the equilibrium stage. Appropriately increasing the diesel injection pressure can shorten the ignition delay and increase the peak cylinder pressure and projected flame area. The closer the injection time is to TDC, the shorter the ignition delay, and the peak cylinder pressure and heat release rate are lower. The pre-injection strategy can effectively shorten the ignition delay and improve the cylinder pressure and heat release rate.
Melatonin Treatment Alleviates Chilling Injury of Loquat Fruit via Modulating ROS Metabolism
Authors: Wan Jiahui , Wu Yanting , Tong Zhihong , Su Wenbing , Lin Hetong , Fan Zhongqi
Published: September 2024

Cold storage is one of the most effective methods to maintain postharvest fruit quality. However, loquat fruits are prone to chilling injury (CI) during cold storage, appearing as symptoms such as browning and pitting, which leads to quality deterioration and economic losses. In this study, the effects of melatonin on CI alleviation and the potential role of reactive oxygen species (ROS) metabolism in loquat fruit were investigated. The results showed that 50 μM melatonin was the optimal concentration to inhibit the increase in CI index and cell membrane permeability. Moreover, compared to control fruits, 50 μM melatonin inhibited the malonaldehyde (MDA) content, O2−. production rate and H2O2 content (ROS accumulation) by 17.8%, 7.2% and 11.8%, respectively, during cold storage. Compared to non-treated loquats, 50 μM melatonin maintained higher levels of 1-diphenyl-2-picrylhydrazyl radical-scavenging ability and reducing power, as well as the contents of ascorbic acid (AsA) and glutathione (GSH). Additionally, 50 μM melatonin enhanced the activities of antioxidant enzymes, such as superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX) by increasing relevant gene expressions. The activities of SOD, CAT and APX were increased by up to 1.1-, 1.1- and 1.1-times (16 d) by melatonin, as compared with the control fruits. These findings indicate that melatonin mitigation of CI is involved in maintaining cellular redox apphomeostasis in loquat fruit during cold storage.
Antistress Effects of Terpinen-4-ol and Compounds of Mimicked Yuzu Synthetic Fragrance in Humans and Mice
Authors: Kitamoto Takuma , Mizushige Takafumi , Xie Xiaonan , Uematsu Taisei , Ogura Risako , Sato Kakeru , Yamazaki Yuki , Matsushita Tsubasa , Hasegawa Hiroshi
Published: September 2024

This study investigated the antistress effects of yuzu synthetic fragrances by employing three experiments on humans and mice using two yuzu synthetic fragrances and five single compounds. We prepared two synthetic fragrances based on the component analysis of two natural yuzu essential oils extracted by cold-pressed and steam-distilled extraction methods. Chromogranin A (CgA) and heart rate (HR) were used as stress indices in human experiments. Immobility time during the forced swim test was used as a stress index in mice experiments. We analyzed brain mechanisms by measuring the expression of neurotrophic factors, brain-derived neurotrophic factor (BDNF), nerve growth factor (NGF), and neurotrophin-3 (NT-3) in the mice experiments. Synthetic yuzu fragrance mimicked steam-distilled oil (SD) significantly reduced participants’ heart rate in experiment 1. In the forced swim test conducted in experiment 2, SD significantly reduced immobility time, and increased the expression of neurotrophic factors BDNF, NGF, and NT-3 in the hippocampus of mice. In experiment 3, focusing on single compounds, terpinen-4-ol significantly reduced immobility time in the forced swim test. These findings indicate that inhalation of SD and terpinen-4-ol has antistress effects. Terpinen-4-ol is a strong candidate for further investigation as a potential stress-reducing agent.
A New Combination of Bifidobacterium bifidum and Lactococcus lactis Strains with Synergistic Effects Alleviates Colitis-Associated Colorectal Cancer
Authors: Shang Jiacui , Liu Lijun , Yang Shuo , Duan Bofan , Xie Shuiqi , Meng Xiangchen
Published: September 2024

Chronic inflammation is a factor in the development of cancer, and probiotics play a role in preventing or treating inflammation as an adjuvant therapy. To investigate potential probiotics for the prevention of colitis-associated colorectal cancer (CAC), Bifidobacterium bifidum H3-R2 and Lactococcus lactis KLDS4.0325 were used to examine the effects on colon cancer cells and in an inflammation-related cancer animal model. The results revealed that B. bifidum H3-R2 in combination with L. lactis KLDS4.0325 caused apoptosis in colon cancer cells by increasing caspase-3 and caspase-9 protein levels, enhancing Bax expression, and lowering Bcl-2 expression. In addition, the combination of the two strains relieved the tissue damage; reduced proinflammatory cytokines, myeloperoxidase (MPO) activity, and hypoxia-inducible factor 1-alpha (HIF-1α) level; upregulated anti-inflammatory cytokines; increased colonic tight junction protein expression; regulated intestinal homeostasis by inhibiting NLRP3 inflammasome signaling pathway; and improved the imbalance of gut microbiota in animal models. Moreover, the combination of the two strains had a greater preventive impact than each strain alone. These findings are supportive of clinical studies and product development of multi-strain probiotic preparations for diseases associated with colitis.
Consumer Acceptance of Novel Lucuma Fruit Ice Cream in the US Market
Authors: Singh Gaganpreet , Kumar Rajesh , Talavera Martin J.
Published: September 2024

This study explored the use of lucuma fruit powder in an ice cream formulation for the US market. Six ice cream prototypes were developed using five different lucuma fruit powder variants. A central location test was conducted with frequent ice cream consumers (n = 106) to assess acceptance, attribute intensity rating, ideal intensity levels, and purchase intent against a control sample with caramel flavor. The mean overall liking score for all lucuma ice creams was moderate. The overall, aroma, and flavor liking scores were significantly higher (p < 0.05) for lucuma ice cream samples, whereas the control sample was liked significantly more for texture. The Terrasoul variant of lucuma ice cream was the most liked among all samples, and the control was liked the least. Only the Terrasoul ice cream sample was successful in delivering significantly strong caramel, fruit, and sweet flavor levels; the other lucuma ice cream samples were more comparable to the control. The inclusion of lucuma powder increased powdery mouthfeel perception, negatively impacting texture liking. Consumers perceived all ice cream samples to be weak in flavor and fell short of delivering ideal levels. The US consumers had low–moderate food neophobia scores for lucuma fruit, with 57% showing interest in buying lucuma ice creams.
Potato Protein-Based Vegan Burgers Enriched with Different Sources of Iron and Fiber: Nutrition, Sensory Characteristics, and Antioxidants before and after In Vitro Digestion
Authors: Kowalczewski Przemysław Łukasz , Wróbel Martyna Maria , Smarzyński Krzysztof , Zembrzuska Joanna , Ślachciński Mariusz , Jeżowski Paweł , Tomczak Aneta , Kulczyński Bartosz , Zielińska-Dawidziak Magdalena , Sałek Karina , Kmiecik Dominik
Published: September 2024

The aim of this research was to develop a technology for the production of plant-based burgers (PBBs) based on potato protein, also containing high content of iron and appropriately selected fats. The produced PBBs were characterized in terms of their nutritional and bioactive properties both before and after the in vitro digestion process. It was found that the produced burger was characterized by high protein content, ranging from 20.80 to 22.16 g/100 g. It was also shown to have a high dietary fiber content, ranging from 8.35 to 9.20 g/100 g. The main fraction of dietary fiber in the tested samples was insoluble fiber, which accounted for approximately 89% of the total fiber content. In addition, noteworthy is the high digestibility of the protein, reaching approximately 95% for the potato fiber used in the formulation, and about 85% for the oat fiber. Produced PBBs also provide significant amounts of iron, with the use of an organic iron source greatly increasing its quantity in the final product. The analyzed antioxidant properties before and after the digestion process showed a tenfold increase in biological activity after digestion, indicating that the examined PBBs may counteract oxidative stress. Analyzing the chemical and biological properties, it is impossible not to assess consumer attractiveness. It has been shown that PBB1, which contains potato fiber and powdered sprouts enriched with ferritin, received the highest attractiveness ratings among respondents.
Microplastics, Polycyclic Aromatic Hydrocarbons, and Heavy Metals in Milk: Analyses and Induced Health Risk Assessment
Authors: Banica Andreea Laura , Radulescu Cristiana , Dulama Ioana Daniela , Bucurica Ioan Alin , Stirbescu Raluca Maria , Stanescu Sorina Geanina
Published: September 2024

The current study aims to develop isolation protocols for several contaminants of emerging concern (i.e., microplastics (MPs), polycyclic aromatic hydrocarbons (PAHs), and heavy metals (HMs)) from different commercial brands and raw milk samples and also to quantify and characterize the risks of these contaminants pose to human health. The quantification, shape, color, and chemical composition of MPs were achieved using optical microscopy, micro-Fourier transform infrared spectroscopy, and scanning electron microscopy coupled with energy-dispersive spectroscopy. Based on the MP dimensions highlighted by the aforementioned techniques, it can be stated that their length ranges between tens of micrometers and a few centimeters; plus, the thickness in some cases reaches more than 15 µm, and the structure of the MPs can be mostly described as a fibriform with a glossy/matte aspect. The polymeric structures identified were polyamides, poly(methyl methacrylate), polyurethane, polyester, and polyethylene. Chemical investigations (PAHs and HMs concentrations) were performed by high-performance liquid chromatography with fluorescence detection and inductively coupled plasma mass spectrometry, respectively. The pollution load index (1.091–7.676) and daily intake of MPs for adults (0.021–1.061 n·kg−1·d−1) and children (0.089–4.420 n·kg−1·d−1) were calculated. It can be concluded that the presence of MPs in milk supports the hypothesis that microplastics can act as carriers for other contaminants (HMs and PAHs), thus increasing the threat to health.
Self-Degradable Rubber Plug for Temporary Plugging and Its Degradation Mechanism
Authors: Yang Fan , Li Fan , Ji Renjing , Yu Xiaorong , Yang Huan , Su Gaoshen
Published: September 2024

A self-degradable rubber plug (SDRP) was developed to address issues in existing crosslinked polymer temporary plugging technology, such as poor self-degradation properties. The synthesis formula was optimized using response surface analysis, resulting in an optimized composition of the SDRP: 13 wt% monomer, 0.02 wt% initiator, 0.7 wt% crosslinker, and 1.8 wt% degradation catalyst. Under the condition of 70–120 °C, the SDRP was transformed from a liquid to a solid gel in 30–110 min; the degradation time was 3–10 days, and the viscosity of the completely degraded solution was lower than 20 mPa·s. At an injection volume of 1 PV SDPR, a breakthrough pressure of 8.34 MPa was achieved. The hydrolysis of the unstable crosslinker was found to have caused the breakage of the SDRP. Over time, the functional groups within the unstable crosslinker underwent hydrolysis due to the combined effects of temperature and the degradation catalyst. This process led to the disruption of crosslinking points, resulting in a gradual deterioration of the network structure. As a consequence, some immobile water was converted into free water. The mobility of water molecules increased until the plug was completely degraded into a viscous liquid. This study enriches the temporary plugging gel system.
Biocompatibility Study of Purified and Low-Temperature-Sterilized Injectable Collagen for Soft Tissue Repair: Intramuscular Implantation in Rats
Authors: Koo Tae-Hoon , Lee Jason K. , Grogan Shawn P. , Ra Ho Jong , D’Lima Darryl D.
Published: September 2024

The clinical application of collagen-based biomaterials is expanding rapidly, especially in tissue engineering and cosmetics. While oral supplements and injectable skin boosters are popular for enhancing skin health, clinical evidence supporting their effectiveness remains limited. Injectable products show potential in revitalizing skin, but safety concerns persist due to challenges in sterilization and the risk of biological contamination. Traditional methods of sterilization (heat and irradiation) can denature collagen. This study addresses these issues by introducing a novel technique: the double filtration and low-temperature steam sterilization of a collagen gel. In vitro tests documented the sterility and confirmed that the collagen did not show cytotoxicity, degradation, integrity, and viscosity characteristics changes after the processing and sterilization. The collagen gel induced new collagen expression and the proliferation of human dermal fibroblasts when the cells were cultured with the collagen gel. An in vivo study found no adverse effects in rats or significant lesions at the implantation site over 13 weeks. These results suggest that this novel method to process collagen gels is a safe and effective skin booster. Advanced processing methods are likely to mitigate the safety risks associated with injectable collagen products, though further research is needed to validate their biological effectiveness and clinical benefits.
A Paintable Small-Molecule Hydrogel with Antimicrobial and ROS Scavenging Activities for Burn Wound Healing
Authors: Ji Qingchun , Chen Kehan , Yi Han , He Bingfang , Jiang Tianyue
Published: September 2024

Delayed wound healing induced by bacterial infection and a persistent inflammatory response remains a great clinical challenge. Herein, we reported a paintable, anti-bacterial, and anti-inflammatory Nap-F3K-CA (Nap-Phe-Phe-Phe-Lys-Caffeic Acid) hydrogel for burn wound management based on caffeic acid (CA)-functionalized short peptides (Nap-Phe-Phe-Phe-Lys). Hydrogels are assembled by non-covalent interactions between gelators, and the incorporation of CA promotes the self-assembly of the hydrogel. After being applied to burn wounds, the hydrogel effectively adapted to irregular wound beds and maintained a moist protective environment at the wound. The Nap-F3K-CA hydrogel can scavenge ROS to relieve oxidative damage and downregulate proinflammatory levels. The Nap-F3K-CA hydrogel also displayed potent antibacterial activity against Gram-positive and Gram-negative bacteria, which reduced the incidence of wound infections. Moreover, the hydrogel exhibited good biocompatibility and hemostatic function. In vivo experiments demonstrated that the Nap-F3K-CA hydrogel significantly accelerated the repair of the skin structure including promoting collagen deposition, vascular regeneration, and hair follicle formation. These findings proved the clinical application potential of the Nap-F3K-CA hydrogel as a promising burn wound dressing.
Assessing Patient Use of and Attitudes toward eHealth Services for Communication with Primary Care Centers in Saudi Arabia and Factors Affecting Usage
Authors: Alessa Tourkiah , Alhussaini Khalid , de Witte Luc
Published: September 2024

Background: This study investigates patients’ use of eHealth services, their awareness of the availability of these services, and their intention to use them in primary care. It also examines patient characteristics and factors that influence the use of these services. Methods: A cross-sectional design using questionnaires was conducted. Based on the unified theory of acceptance and use of technology (UTAUT), the participants rated the two most common services. Descriptive analyses and linear correlation analyses were performed. A simple linear regression was conducted to identify factors influencing the participants’ intention to use eHealth services. Results: In total, 1203 participants with an average age of 43.7 years were surveyed. The participants’ usage rates varied, with the lowest at 2.4%, for measuring vital signs, and the highest at 47.4%, for booking appointments. The intentions to use the services ranged from 22.5%, for video consultations, to 46.6%, for prescription refill requests. Approximately 20% of the respondents were unaware of each service’s availability. Positive associations were found between all the constructs and the intention to use online services, with a younger age being the most significant factor. Conclusions: The use of and intention to use eHealth services varied greatly. The participants were often unaware of the availability of these services. Promoting the availability and benefits of eHealth services could enhance patient engagement in primary care settings.
The Effect of Humic-Based Biostimulants on the Yield and Quality Parameters of Chili Peppers
Authors: Mezeyová Ivana , Kollárová Ivana , Golian Marcel , Árvay Július , Mezey Ján , Šlosár Miroslav , Galovičová Lucia , Rosa Robert , Bakalár Martin , Horečná Tereza
Published: September 2024

Chili peppers are globally cultivated for their rich bioactive compound profile. This study investigates the impact of two biostimulants, Humix® and Energen, on Capsicum chinense ‘Habanero Orange’ and Capsicum annuum ‘Kristian’, focusing on quantitative and qualitative parameters. Conducted over two years with three annual harvests, the research assesses the effects of biostimulant application on yield, fresh fruit number, fruit weight, drying ratio, capsaicin, dihydrocapsaicin, and ascorbic acid content (via HPLC-DAD analysis), as well as carotenoid levels (via spectrophotometric analysis). Biostimulant application significantly increased (p ≤ 0.05) total yields and capsaicin levels. Harvest timing also influenced dihydrocapsaicin and capsaicin levels, with the third harvest showing the highest values (p ≤ 0.001). The effects on ascorbic acid and carotenoids were variable and depended on genotype, harvest, and treatment. Thus, our study provides insights into the dynamic responses of Capsicum species to biostimulants under variable climatic conditions, contributing new knowledge to agricultural practices and the scientific understanding of biostimulant effects in Capsicum production.
A Comprehensive Bibliometric Analysis of Real Estate Research Trends
Authors: El Bied Salma , Ros Mcdonnell Lorenzo , de-la-Fuente-Aragón Ma Victoria , Ros Mcdonnell Diego
Published: September 2024

Real estate, characterized by its diverse and complex nature, presents a multifaceted research domain. It encompasses various topics and challenges, making it both content-wise challenging and multidimensional. This study aims to conduct a knowledge mapping of the literature in the real estate field using a sample of 9700 document articles published between 1929 and 2023 based on publications indexed in the Web of Science database. This study utilizes the software SciMAT (version 1.1.04) to demonstrate hot keywords and trends in this field and additionally employs the VOSviewer (version 1.6.19) tool to analyze keywords, countries, authors, and sources. Authors reveal a growing interest in real estate literature, with the USA contributing the most publications, while relatively few originate from Africa and South America. This study investigates the strategic themes and the scientific evolution structure, provides a comprehensive examination of the current state of real estate literature, and helps in understanding its development. It offers a valuable reference point for future research in this domain.
Sports Injury Surveillance Systems: A Scoping Review of Practice and Methodologies
Authors: Costello Damien , Daly Ed , Ryan Lisa
Published: September 2024

Background: Injury prevention/reduction strategies are driven by data collected through injury surveillance systems. The aim of this review was to describe injury surveillance systems that are used for ongoing surveillance in either a professional or amateur sporting environment. This was an update to a review done in 2015 to determine the gaps in injury surveillance. Methods: A systematic search process of five databases (MEDLINE, CINAHL, EMBASE, SCOPUS and ProQuest) was used to discover published research that presented methodological data about the injury surveillance systems implemented by clubs and organisations for ongoing surveillance. Inclusion criteria centred on the population under surveillance and the ongoing nature of that activity. Data extracted and summarised included the level of athlete under surveillance, the data collection mechanism and the personnel involved, the injury definitions applied and the date and country of origin to provide a comprehensive picture of the systems. Results: A total of 21 systems were documented as being used in ongoing injury surveillance, with 57% of these exclusively in the professional/elite landscapes and 33% at the amateur level. Surveillance systems cater for one sport per athlete entry so there is a gap in research for multi-sport athletes at the amateur level, especially where there is no early specialisation in a multi-sport participation environment. Conclusions: Research in this area will lead to a better understanding of subsequent injury risk for multi-sport athletes who have a higher athlete exposure than single-sport athletes.
Effects of Physical Exercise Input on the Exercise Adherence of College Students: The Chain Mediating Role of Sports Emotional Intelligence and Exercise Self-Efficacy
Authors: An Dongzhen , Pan Jianhua , Ran Feng , Bai Donghuan , Zhang Jia
Published: September 2024

Objective: The aims of this study were to investigate the effects and mechanisms of physical exercise input, sports emotional intelligence, and sports self-efficacy on exercise adherence, and to examine the chain-mediating role of sports emotional intelligence→sports self-efficacy. Methods: The Physical Exercise Input Scale, Exercise Adherence Scale, Sports Emotional Intelligence Scale, and Sports Self-Efficacy Scale were used to investigate 1390 college students in three universities in the Henan Province. Results: (1) Physical exercise input was a significant positive predictor of exercise adherence (β = 0.29, t = 5.78, p < 0.001); (2) sports emotional intelligence and sports self-efficacy mediated the relationship between physical exercise input and exercise adherence; (3) physical exercise input influenced exercise adherence through the separate mediating role of sports emotional intelligence (β = 0.10, t = 5.98, p < 0.001), the separate mediating role of sports self-efficacy (β = 0.13, t = 2.64, p < 0.01), and the chain mediating role of sports emotional intelligence→sports self-efficacy (β = 0.09, t = 2.80, p < 0.01). Conclusions: (1) Physical exercise input can positively predict the level of sports emotional intelligence and sports self-efficacy of college students; (2) Physical exercise input can not only directly influence college students’ exercise adherence but can also indirectly influence it through sports emotional intelligence or sports self-efficacy levels alone, as well as through the chain mediation of the two.
Characterization of Abrasive Grain Signal of Oil Detection Sensor Based on LC Resonance Wireless Transmission
Authors: Zhang Shaoxuan , Zhang Zuo , Bai Chenzhao , Hu Shukui , Wang Jizhe , Wang Chenyong , Zhang Hongpeng
Published: September 2024

Friction in marine engineering machinery produces abrasive particles containing valuable information. By employing oil detection technology, we can analyze these particles to monitor and diagnose mechanical system faults. This paper introduces an inductive oil detection sensor and wireless signal transmission circuit. The sensor utilizes two opposing solenoid coils of the same specifications, with the detection coil connected to a chip capacitor to form an LC resonant unit. The designed wireless transmission circuit wirelessly transmits a sensing signal from a detection coil to a receiving coil to detect metal particles in oil. This paper deduces the sensor’s inductance principle and simulates the magnetic field distribution using finite element simulation software. Through experiments, the optimal excitation frequency, coil spacing, and oil sample flow path location were determined. The sensor successfully detected 55 μm iron particles and 138 μm copper particles in a 1 mm microfluidic channel. With its simple structure, distinct signal characteristics, and high sensitivity, the sensor is suitable for detecting metal abrasive particles in hydraulic oil, providing a new approach for wireless transmission in oil detection sensors.
Carbon and Nitrogen Sources Influence Parasitic Responsiveness in Trichoderma atroviride NI-1
Authors: García-Sánchez Víctor Javier , Sánchez-López Karina Lizbeth , Esquivel Méndez Juana Jazmín , Sánchez-Hernández Daniel , Cervantes-Chávez José Antonio , Landeros-Jaime Fidel , Mendoza-Mendoza Artemio , Vega-Arreguín Julio Cesar , Esquivel-Naranjo Edgardo Ulises
Published: September 2024

Parasitic species of Trichoderma use hydrolytic enzymes to destroy the host cell wall. Preferent carbon and nitrogen sources suppress the expression of genes related to parasitism. Here, different nutrients were evaluated in the parasitic isolated NI-1, which was identified as Trichoderma atroviride. The genes cbh1 and chb2 (cellobiohydrolases), bgl3.1 (endoglucanase), and pra1 and prb1 (proteinases) were poorly expressed during the interaction between NI-1 and Phytophthora capsici on PDA. However, gene expression improved on minimal medium with preferent and alternative carbon sources. Dextrin and glucose stimulated higher transcript levels than cellulose, sucrose, and glycerol. Also, ammonium stimulated a stronger parasitic responsiveness than the alternative nitrogen sources. During interaction against different phytopathogens, NI-1 detects their host differentially from a distance due to the cbh1 and cbh2 genes being only induced by P. capsici. The pra1 and ech42 genes were induced before contact with Botrytis cinerea and Rhizoctonia solani, while when confronted with P. capsici they were stimulated until contact and overgrowth. The prb1 and bgl3.1 genes were induced before contact against the three-host assayed. Overall, T. atroviride prefers to parasitize and has the capacity to distinguish between an oomycete and a fungus, but nutrient quality regulates its parasitic responsiveness.
Digital Orientation and Manufacturing Firms’ Green Innovation Performance: The Mediating Role of Green Competence
Authors: Dogbe Courage Simon Kofi , Marwa Nyankomo
Published: September 2024

This study assessed the mediating role of green competence in the relationship between digital orientation and green innovation performance among Chinese manufacturing firms. This study gathered data from 227 manufacturing firms located in the Jiangsu Province of China. Data were gathered using a structured questionnaire and analyzed using a covariance-based structural equation modeling in Amos (v.23). The findings revealed that digital orientation had a direct effect on manufacturing firms’ green innovation performance. This study also reveals that digital orientation had a direct positive effect on green competence. Also, green competence had a direct effect on green innovation performance. Finally, it was identified that green competence partially mediated the relationship between digital orientation and manufacturing firms’ green innovation performance.