bluuebunny/crossref_metadata_2025 huggingface dataset:
What to Do When There Is Something Unexpected?
Authors: Ivan Vlad , Lighezan Daniel-Florin , Ardelean Melania , Balteș Nicoleta , Faur Alexandra , Ciubotaru Paul-Gabriel , Cutina-Morgovan Adina-Flavia , Buzaș Roxana
Published: January 2024

Background: Myocardial infarction is currently the leading cause of death worldwide, followed by malignant neoplasms. The presence of both within the same patient obviously increases the risk of death, as many coronary events are detected in patients diagnosed with cancer. Diagnosis of an occult digestive cancer in the acute phase of myocardial infarction is most frequently prompted by a hemorrhagic complication. Case summary: This case features an 81-year-old male patient diagnosed with acute myocardial infarction, treated with primary percutaneous intervention (PCI), who developed post-stenting hemorrhagic complications in the first 24 h due to the presence of two different concomitant malignant neoplasms. The outcome was favorable in the acute phase, even if de-escalation therapy was given immediately post-stenting, and intrastent residual thrombotic risk was high. Conclusions: The presence of bleeding complications in patients with acute myocardial infarction should mobilize resources in search of a neoplastic cause, especially a digestive one. However, other locations should be looked for, depending on the source of bleeding.
Simulation and Experimental Study on the Effect of Superheat on Solidification Microstructure Evolution of Billet in Continuous Casting
Authors: Tian Nan , Zhang Guifang , Yan Peng , Li Pengchao , Feng Zhenhua , Wang Xiaoliang
Published: January 2024

The control of the solidification structure of a casting billet is directly correlated with the quality of steel. Variations in superheat can influence the transition from columnar crystals to equiaxed crystals during the solidification process, subsequently impacting the final solidification structure of the billet. In this study, a model of microstructure evolution during billet solidification was established by combining simulation and experiment, and the dendrite growth microstructure evolution during billet solidification under different superheat was studied. The results show that when the superheat is 60 K, the complete solidification time of the casting billet from the end of the 50 mm section is 252 s, when the superheat is 40 K, the complete solidification time of the casting billet is 250 s, and when the superheat is 20 K, the complete solidification time of the casting billet is 245 s. When the superheat is 20 K, the proportion of the equiaxed crystal region is higher—the highest value is 53.35%—and the average grain radius is 0.84556 mm. The proportion of the equiaxed crystal region decreases with the increase of superheat. When the superheat is 60 K, the proportion of the equiaxed crystal region is the lowest—the lowest value is 46.27%—and the average grain radius is 1.07653 mm. Proper reduction of superheat can obviously reduce the size of equiaxed crystal, expand the area of equiaxed crystal and improve the quality of casting billet.
Microstructural and Textural Evolution of Cold-Drawn Mg–Gd Wires during Annealing Treatment
Authors: Sun Liuxia , Bai Jing , Xue Feng , Yan Kai
Published: January 2024

In addition to cold drawing, the process of annealing is also essential in the preparation of Mg-4.7 wt%Gd (G4.7) alloy wires. The effect of annealing treatment on the recrystallized microstructure and texture of cold-drawn G4.7 wires was investigated. The results demonstrate that the uniformity and regularity of the recrystallized grains, as well as the annealing texture, impact the follow-up cold drawing performance. When the as-drawn G4.7 wires were annealed at 375 °C, the recrystallized grains were refined, accompanied by uniformity and regularity. Accordingly, the G4.7 wire had a good subsequent drawing deformability, with a maximum accumulative true strain (ATS) of 144%. Additionally, the evolution of the microstructure was consistent with the evolution of the texture. While annealing at a lower temperature (325 °C), the {0002} basal texture of the G4.7 wire was weak, forming the main texture component <101¯0>//DD (the drawing direction). With the increase in temperature, the basal texture was gradually strengthened and the texture component transformed from <101¯0>//DD to a recrystallized texture based on <112¯0>//DD. Even under high-temperature annealing, the G4.7 wire was still affected by the cold-drawn deformation texture and could not fully recover to the as-extruded texture, thus causing a decrease in the subsequent drawing performance.
Optimizing the Strength and Toughness of V/Mo-Modified 0.22C–5.24Mn Steel by Short-Time Partial Austenitization Process
Authors: Zheng Haoqing , Liu Gang , Tong Shuai , Su Guanqiao , Liang Xiaokai , Sun Xinjun
Published: January 2024

In order to obtain the good match between yield strength and low-temperature toughness, the short-time partial austenitization (SPA) process was employed for V/Mo-bearing 0.22C–5.24Mn steel. The initial microstructure after intercritical tempering was dual-phase ferrite and reversed austenite (RA), while the final microstructure consisted of ferrite, RA, and secondary martensite (SM) after being subjected to the SPA process. (V, Mo)C with disclike morphology mainly precipitated during intercritical tempering, and the aspect ratio of particles decreased, leading to the appearance of near-spherical morphology. After being subjected to SPA process, the resultant multiphase hierarchical microstructure (three layers: outer layer of ferrite, interlayer of SM, and inner layer of RA) enabled a high yield strength of 1097 MPa, a total elongation of 14%, and an impressive impact energy of 33.3 J at −20 °C. The strengthening contribution of (V, Mo)C precipitation was estimated to be about 108 MPa.
Morphology of Microchips in the Surface Finishing Process Utilizing Abrasive Films
Authors: Tandecka Katarzyna , Kacalak Wojciech , Wiliński Maciej , Wieczorowski Michał , Mathia Thomas G.
Published: January 2024

In this study, the surface of new lapping films was analyzed, and the lapping finishing process was applied to RG7 tin bronze alloy. The research focused on examining lapping films with electrocorundum grains of nominal sizes 30, 12, and 9 μm, commonly used for achieving smooth surfaces. The manufacturing process involves placing abrasive grains and binder onto a polyester tape, resulting in a heterogeneous distribution of abrasive grains. The study investigates the impact of this random distribution on the performance of lapping films during material removal. Scanning electron microscopy was used to analyze the surface structure of abrasive films, revealing distinctive structures formed by the specific aggregation of abrasive grains. This study explores the influence of different nominal grain sizes on surface finish and aims to optimize lapping processes for diverse applications. The research also delves into microchip analysis, examining the products of the lapping film finishing process. Microchips were observed directly on the abrasive tool surface, revealing insights into their morphology and distribution. The chip segmentation frequency was determined, and they amounted to approximately 0.8 to 3 MHz; these are very high frequencies, which are unique for known chip-forming processes.
Fusion Q-Learning Algorithm for Open Shop Scheduling Problem with AGVs
Authors: Wen Xiaoyu , Zhang Haobo , Li Hao , Wang Haoqi , Ming Wuyi , Zhang Yuyan , Zhang Like
Published: January 2024

In accordance with the actual production circumstances of enterprises, a scheduling problem model is designed for open-shop environments, considering AGV transport time. A Q-learning-based method is proposed for the resolution of such problems. Based on the characteristics of the problem, a hybrid encoding approach combining process encoding and AGV encoding is applied. Three pairs of actions are constituted to form the action space. Decay factors and a greedy strategy are utilized to perturb the decision-making of the intelligent agent, preventing it from falling into local optima while simultaneously facilitating extensive exploration of the solution space. Finally, the proposed method proved to be effective in solving the open-shop scheduling problem considering AGV transport time through multiple comparative experiments.
Machine-Learning-Based Approaches for Multi-Level Sentiment Analysis of Romanian Reviews
Authors: Briciu Anamaria , Călin Alina-Delia , Miholca Diana-Lucia , Moroz-Dubenco Cristiana , Petrașcu Vladiela , Dascălu George
Published: January 2024

Sentiment analysis has increasingly gained significance in commercial settings, driven by the rising impact of reviews on purchase decision-making in recent years. This research conducts a thorough examination of the suitability of machine learning and deep learning approaches for sentiment analysis, using Romanian reviews as a case study, with the aim of gaining insights into their practical utility. A comprehensive, multi-level analysis is performed, covering the document, sentence, and aspect levels. The main contributions of the paper refer to the in-depth exploration of multiple sentiment analysis models at three different textual levels and the subsequent improvements brought with respect to these standard models. Additionally, a balanced dataset of Romanian reviews from twelve product categories is introduced. The results indicate that, at the document level, supervised deep learning techniques yield the best outcomes (specifically, a convolutional neural network model that obtains an AUC value of 0.93 for binary classification and a weighted average F1-score of 0.77 in a multi-class setting with 5 target classes), albeit with increased resource consumption. Favorable results are achieved at the sentence level, as well, despite the heightened complexity of sentiment identification. In this case, the best-performing model is logistic regression, for which a weighted average F1-score of 0.77 is obtained in a multi-class polarity classification task with three classes. Finally, at the aspect level, promising outcomes are observed in both aspect term extraction and aspect category detection tasks, in the form of coherent and easily interpretable word clusters, encouraging further exploration in the context of aspect-based sentiment analysis for the Romanian language.
On the Equilibrium Locus of a Parameterized Dynamical System with Independent First Integrals
Authors: Kaminski Yirmeyahu , Lochak Pierre
Published: January 2024

For a family of dynamical systems with k>0 independent first integrals evolving in a compact region of a Euclidean space, we study the equilibrium locus. We show that under mild and generic conditions, it is a smooth manifold that can be viewed as the total space of a certain fiber bundle and that this bundle comes equipped with a natural connection. We then proceed to show parallel transport for this connection does exist and explore some of its properties. In particular, we elucidate how one can to some extent measure the variation of the system eigenvalues restricted to a given fiber.
The Solution of the Extended 16th Hilbert Problem for Some Classes of Piecewise Differential Systems
Authors: Baymout Louiza , Benterki Rebiha , Llibre Jaume
Published: January 2024

The limit cycles have a main role in understanding the dynamics of planar differential systems, but their study is generally challenging. In the last few years, there has been a growing interest in researching the limit cycles of certain classes of piecewise differential systems due to their wide uses in modeling many natural phenomena. In this paper, we provide the upper bounds for the maximum number of crossing limit cycles of certain classes of discontinuous piecewise differential systems (simply PDS) separated by a straight line and consequently formed by two differential systems. A linear plus cubic polynomial forms six families of Hamiltonian nilpotent centers. First, we study the crossing limit cycles of the PDS formed by a linear center and one arbitrary of the six Hamiltonian nilpotent centers. These six classes of PDS have at most one crossing limit cycle, and there are systems in each class with precisely one limit cycle. Second, we study the crossing limit cycles of the PDS formed by two of the six Hamiltonian nilpotent centers. There are systems in each of these 21 classes of PDS that have exactly four crossing limit cycles.
Therapeutic Drug Monitoring for Biologic and Small-Molecule Therapies for Inflammatory Bowel Disease
Authors: Dutt Krishneel , Vasudevan Abhinav
Published: January 2024

Background: Inflammatory bowel disease (IBD), encompassing ulcerative colitis and Crohn’s disease, necessitates long-term medical therapy to manage symptoms and prevent complications. Therapeutic drug monitoring (TDM) has emerged as a strategy to optimize treatment efficacy, particularly with anti-tumour necrosis factor (anti-TNF) alpha drugs. This review explores the role of TDM for non-anti-TNF advanced therapies in IBD, focusing on vedolizumab, ustekinumab, tofacitinib, upadacitinib, risankizumab and ozanimod. Methods: The literature search, conducted through OVID (Medline) and PubMed, delves into proactive versus reactive TDM, timing of monitoring and methods for measuring drug levels and anti-drug antibodies. Results: While ustekinumab and vedolizumab exhibit exposure–response relationships, consensus on target levels and the role of TDM adjustments remains elusive. Limited data on risankizumab suggest a dose-dependent response, while for small molecule therapies (janus kinase inhibitors and ozanimod), the absence of real-world data and commercially available TDM tools pose challenges. Conclusion: At present, with the available data, there is a limited role for TDM in non-anti-TNF biologic and small-molecule therapies. This review underscores the need for further research to delineate the utility of TDM in guiding treatment decisions for these agents.
Effect of Cold Rolling and Cryogenic Treatment on the Microstructure and Mechanical Properties of Fe–32Ni Alloy
Authors: Sun Dongyun , Li Jianguang , Hao Xiaohong , Wang Mingming , Li Dongdong , Sun Xiaowen , Yang Zhinan , Wang Tiansheng , Zhang Fucheng
Published: January 2024

In this work, the effects of cold rolling (CR) and cold rolling–cryogenic treatment (CR–CT) on the microstructure and mechanical properties of Fe–32Ni alloy were studied via optical microscopy methods, OM, SEM, XRD, TEM, tensile strength and hardness tester, and tensile testing. The results reveal the grain refinement in the alloy after rolling deformation. When the deformation is higher than 85%, the polygonal austenite grains become layered, and a small amount of martensite forms. Because of the inhibitory effect of cold-rolling deformation before cryogenic treatment on martensitic transformation, the amount of martensite form phase after cryogenic treatment decreases with the increase of deformation. The hardness and strength of the sample, independent of whether the cryogenic treatment is performed, increase with the increase of deformation degree. Under the same deformation rate, the hardness of the CR–CT sample is higher than that of the CR sample, which is related to the hard martensite phase with high dislocation density obtained during cryogenic treatment. The strain hardening behavior of the sample is greatly affected by the deformation degree. With the increase of true strain, the work hardening exponent of CR and CR–CT samples undergoing severe plastic deformation is lower than that at small deformation degree and low dislocation density, which is attributed to the earlier entanglement of high dislocations in CR and CR–CT samples with large deformation degrees.
A Novel Channel Preparation Scheme to Optimize Program Disturbance in Three-Dimensional NAND Flash Memory
Authors: You Kaikai , Jin Lei , Jia Jianquan , Huo Zongliang
Published: January 2024

The program disturbance characteristics of three-dimensional (3D) vertical NAND flash cell array architecture pose a critical reliability challenge due to the lower unselected word line (WL) pass bias (Vpass) window. In other words, the key contradiction of program disturbance is that the operational Vpass during the program’s performance cannot be too high or too low. For instance, the 3D NAND program’s operation string needs a lower Vpass bias to suppress unselected WL Vpass bias-induced Fowler–Nordheim tunneling (FN tunneling), but for the inhibited string, the unselected WL needs a higher Vpass bias to suppress selected WL program bias (Vpgm)-induced FN tunneling. In this paper, a systematical insight into the relationship between the channel potential and channel electron density is given. Based on this intensive investigation, we studied a novel channel preparation scheme using “Gate-induced drain leakage (GIDL) pre-charge”. Our methodology does not require the introduction of any new structures in 3D NAND, or changes in the operational Vpass bias. Instead, the potential on the unselected channel is enhanced by exploiting the holes generated by the GIDL operation effectively, leading to significantly suppressed program disturbance and a larger pass disturb window. To validate the effectiveness of the “GIDL pre-charge” method, TCAD simulation and real silicon data are used.
Enhanced Molecularly Imprinted Fluorescent Test Strip for Rapid and Visual Detection of Norfloxacin via a Smartphone
Authors: Hu Bo , Zhao Wenshi , Chen Li , Liu Yang , Ma Zhongfei , Yan Yongsheng , Meng Minjia
Published: January 2024

Paper-based test strips with on-site visual detection have become a hot spot in the field of target detection. Yet, low specific surface area and uneven deposition limit the further application of test strips. Herein, a novel “turn-on” ratio of molecularly imprinted membranes (Eu@CDs-MIMs) was successfully prepared based on a Eu complex-doped polyvinylidene fluoride membrane for the selective, rapid and on-site visual detection of norfloxacin (NOR). The formation of surface-imprinted polymer-containing carbon dots (CDs) improves the roughness and hydrophilicity of Eu@CDs-MIMs. Fluorescence lifetimes and UV absorption spectra verified that the fluorescence enhancement of CDs is based on the synergistic effect of charge transfer and hydrogen bonding between CDs and NOR. The fluorescent test strip showed a linear fluorescent response within the concentration range of 5–50 nM with a limit of detection of 1.35 nM and a short response time of 1 min. In comparison with filter paper-based test strips, Eu@CDs-MIMs exhibit a brighter and more uniform fluorescent color change from red to blue that is visible to the naked eye. Additionally, the applied ratio fluorescent test strip was combined with a smartphone to translate RGB values into concentrations for the visual and quantitative detection of NOR and verified the detection results using high-performance liquid chromatography. The portable fluorescent test strip provides a reliable approach for the rapid, visual, and on-site detection of NOR and quinolones.
Enhanced and Sustainable Removal of Indoor Formaldehyde by Naturally Porous Bamboo Activated Carbon Supported with MnOx: Synergistic Effect of Adsorption and Oxidation
Authors: Li Zhenrui , Li Yujun , Li Shijie , Ma Jianfeng , Ma Qianli , Wang Zhihui , Wang Jiajun , Long Keying , Liu Xing’e
Published: January 2024

Novel bamboo activated carbon (BAC) catalysts decorated with manganese oxides (MnOx) were prepared with varying MnOx contents through a facile one-step redox reaction. Due to the physical anchoring effect of the natural macropore structure for catalyst active components, homogeneous MnOx nanoparticles (NPs), and high specific surface area over catalyst surface, the BAC@MnOx-N (N = 1, 2, 3, 4, 5) catalyst shows encouraging adsorption and catalytic oxidation for indoor formaldehyde (HCHO) removal at room temperature. Dynamic adsorption and catalytic activity experiments were conducted. The higher Smicro (733 m2/g) and Vmicro/Vt (82.6%) of the BAC@MnOx-4 catalyst could facilitate its excellent saturated and breakthrough adsorption capacity (5.24 ± 0.42 mg/g, 2.43 ± 0.22 mg/g). The best performer against 2 ppm HCHO is BAC@MnOx-4 catalyst, exhibiting a maximum HCHO removal efficiency of 97% for 17 h without any deactivation as RH = 0, which is higher than those of other MnOx-based catalysts. The average oxidation state and in situ DRIFTS analysis reveal that abundant oxygen vacancies on the BAC@MnOx-4 catalyst could be identified as surface-active sites of decomposing HCHO into the intermediate species (dioxymethylene and formate). This study provides a potential approach to deposit MnOx nanoparticles onto the BAC surface, and this hybrid BAC@MnOx material is promising for indoor HCHO removal at room temperature.
In Situ Monitoring of Non-Thermal Plasma Cleaning of Surfactant Encapsulated Nanoparticles
Authors: Li Gengnan , Zakharov Dmitri N. , Sikder Sayantani , Xu Yixin , Tong Xiao , Dimitrakellis Panagiotis , Boscoboinik Jorge Anibal
Published: January 2024

Surfactants are widely used in the synthesis of nanoparticles, as they have a remarkable ability to direct their growth to obtain well-defined shapes and sizes. However, their post-synthesis removal is a challenge, and the methods used often result in morphological changes that defeat the purpose of the initial controlled growth. Moreover, after the removal of surfactants, the highly active surfaces of nanomaterials may undergo structural reconstruction by exposure to a different environment. Thus, ex situ characterization after air exposure may not reflect the effect of the cleaning methods. Here, combining X-ray photoelectron spectroscopy, in situ infrared reflection absorption spectroscopy, and environmental transmission electron microscopy measurements with CO probe experiments, we investigated different surfactant-removal methods to produce clean metallic Pt nanoparticles from surfactant-encapsulated ones. It was demonstrated that both ultraviolet-ozone (UV-ozone) treatment and room temperature O2 plasma treatment led to the formation of Pt oxides on the surface after the removal of the surfactant. On the other hand, when H2 was used for plasma treatment, both the Pt0 oxidation state and nanoparticle size distribution were preserved. In addition, H2 plasma treatment can reduce Pt oxides after O2-based treatments, resulting in metallic nanoparticles with clean surfaces. These findings provide a better understanding of the various options for surfactant removal from metal nanoparticles and point toward non-thermal plasmas as the best route if the integrity of the nanoparticle needs to be preserved.
The Role of Diet and Gut Microbiota in Alzheimer’s Disease
Authors: Dissanayaka D. M. Sithara , Jayasena Vijay , Rainey-Smith Stephanie R. , Martins Ralph N. , Fernando W. M. A. D. Binosha
Published: January 2024

Alzheimer’s disease (AD), the most prevalent form of dementia, is characterized by the accumulation of amyloid-beta (Aβ) plaques and hyperphosphorylated tau tangles. Currently, Alzheimer’s disease (AD) impacts 50 million individuals, with projections anticipating an increase to 152 million by the year 2050. Despite the increasing global prevalence of AD, its underlying pathology remains poorly understood, posing challenges for early diagnosis and treatment. Recent research suggests a link between gut dysbiosis and the aggregation of Aβ, the development of tau proteins, and the occurrence of neuroinflammation and oxidative stress are associated with AD. However, investigations into the gut–brain axis (GBA) in the context of AD progression and pathology have yielded inconsistent findings. This review aims to enhance our understanding of microbial diversity at the species level and the role of these species in AD pathology. Additionally, this review addresses the influence of confounding elements, including diet, probiotics, and prebiotics, on AD throughout different stages (preclinical, mild cognitive impairment (MCI), and AD) of its progression.
The Effect of Alginate Encapsulated Plant-Based Carbohydrate and Protein Supplementation on Recovery and Subsequent Performance in Athletes
Authors: Nielsen Lotte L. K. , Lambert Max Norman Tandrup , Haubek Dorte , Bastani Nasser E. , Skålhegg Bjørn S. , Overgaard Kristian , Jensen Jørgen , Jeppesen Per Bendix
Published: January 2024

The main purpose of this study was to investigate the effect of a novel alginate-encapsulated carbohydrate–protein (CHO–PRO ratio 2:1) supplement (ALG) on cycling performance. The ALG, designed to control the release of nutrients, was compared to an isocaloric carbohydrate-only control (CON). Alginate encapsulation of CHOs has the potential to reduce the risk of carious lesions. Methods: In a randomised cross-over clinical trial, 14 men completed a preliminary test over 2 experimental days separated by ~6 days. An experimental day consisted of an exercise bout (EX1) of cycling until exhaustion at W~73%, followed by 5 h of recovery and a subsequent time-to-exhaustion (TTE) performance test at W~65%. Subjects ingested either ALG (0.8 g CHO/kg/hr + 0.4 g PRO/kg/hr) or CON (1.2 g CHO/kg/hr) during the first 2 h of recovery. Results: Participants cycled on average 75.2 ± 5.9 min during EX1. Levels of plasma branched-chain amino acids decreased significantly after EX1, and increased significantly with the intake of ALG during the recovery period. During recovery, a significantly higher plasma insulin and glucose response was observed after intake of CON compared to ALG. Intake of ALG increased plasma glucagon, free fatty acids, and glycerol significantly. No differences were found in the TTE between the supplements (p = 0.13) nor in the pH of the subjects’ saliva. Conclusions: During the ALG supplement, plasma amino acids remained elevated during the recovery. Despite the 1/3 less CHO intake with ALG compared to CON, the TTE performance was similar after intake of either supplement.
Comment on Meneghini et al. The Impact of Nutritional Therapy in the Management of Overweight/Obese PCOS Patient Candidates for IVF. Nutrients 2023, 15, 4444
Authors: Cetkovic Nenad , Scarlata Giuseppe Guido Maria , Abenavoli Ludovico
Published: January 2024

We read with great interest the recent article by Meneghini et al. on the assessment of the effects of different alimentary regimens, included Mediterranean diet (MD), on polycystic ovary syndrome (PCOS) patients prior to in vitro fertilization cycles [...]
Factors Associated with Anemia and Iron Deficiency during Pregnancy: A Prospective Observational Study in Japan
Authors: Habe Sarasa , Haruna Megumi , Yonezawa Kaori , Usui Yuriko , Sasaki Satoshi , Nagamatsu Takeshi , Fujita Megumi , Suetsugu Yoshiko , Ohori Riko , Tanaka Moeko , Aoyama Satoko
Published: January 2024

Gestational anemia (GA) is a global health concern with a remarkably high prevalence in Japan, which is associated with various maternal and neonatal outcomes. This study aimed to explore whether GA and non-anemic iron deficiency (NAID) during the third trimester is associated with maternal characteristics, nutrient intake, low birth weight (LBW), and preterm birth. Participants were categorized into GA, NAID, and normal groups, based on serum ferritin and hemoglobin levels. Nutrient intake was assessed using the Brief Diet History Questionnaire. Data from 317 pregnant women were analyzed, including 110 (34.7%), 151 (47.6%), and 56 (17.6%) women in the GA, NAID, and normal groups, respectively. Factors associated with GA included being multipara (p < 0.001) and not taking any type of iron supplements in the third trimester (p = 0.043). The normal group had a significantly higher proportion of preterm birth and LBW than the GA and NAID groups. The GA group had a significantly higher energy intake than the normal group (p = 0.044). Overall, energy and micronutrient intake were significantly below the estimated average requirement in the dietary reference intakes for Japanese. Health care professionals need to consider nutritional advice that can prevent GA by focusing on overall micronutrients, not just energy intake.
Twelve Weeks of Daily Lentil Consumption Improves Fasting Cholesterol and Postprandial Glucose and Inflammatory Responses—A Randomized Clinical Trial
Authors: Chamberlin Morgan L. , Wilson Stephanie M.G. , Gaston Marcy E. , Kuo Wan-Yuan , Miles Mary P.
Published: January 2024

Lentils have potential to improve metabolic health but there are limited randomized clinical trials evaluating their comprehensive impact on metabolism. The aim of this study was to assess the impact of lentil-based vs. meat-based meals on fasting and postprandial measures of glucose and lipid metabolism and inflammation. Thirty-eight adults with an increased waist circumference (male ≥ 40 inches and female ≥ 35 inches) participated in a 12-week dietary intervention that included seven prepared midday meals totaling either 980 g (LEN) or 0 g (CON) of cooked green lentils per week. Linear models were used to assess changes in fasting and postprandial markers from pre- to post-intervention by meal group. Gastrointestinal (GI) symptoms were assessed through a survey randomly delivered once per week during the intervention. We found that regular consumption of lentils lowered fasting LDL (F = 5.53, p = 0.02) and total cholesterol levels (F = 8.64, p < 0.01) as well as postprandial glucose (β = −0.99, p = 0.01), IL-17 (β = −0.68, p = 0.04), and IL-1β (β = −0.70, p = 0.03) responses. GI symptoms were not different by meal group and all symptoms were reported as “none” or “mild” for the duration of the intervention. Our results suggest that daily lentil consumption may be helpful in lowering cholesterol and postprandial glycemic and inflammatory responses without causing GI stress. This information further informs the development of pulse-based dietary strategies to lower disease risk and to slow or reverse metabolic disease progression in at-risk populations.
The Value of an Ecological Approach to Improve the Precision of Nutritional Assessment: Addressing Contributors and Implications of the “Multiple Burdens of Malnutrition”
Authors: Raiten Daniel J. , Steiber Alison L. , Dary Omar , Bremer Andrew A.
Published: January 2024

Globally, children are exposed to multiple health risks associated with diet and nutrition. Rather than simply being a condition of having too much or too little food, malnutrition is more a syndrome comprising multiple burdens of coexisting and reciprocal malnutrition, infection, or other conditions. Importantly, children with such syndromes (e.g., stunting and anemia, which are neither specific nor necessarily sensitive to nutritional status) are more likely to also have irreversible functional outcomes such as poor growth, impaired immune function, or cognitive delays. The global health community has identified nutrition-related targets (e.g., Sustainable Development Goals (SDGs) and World Health Organization (WHO) Global Nutrition Targets) that, for multiple reasons, are difficult to address. Moreover, as the complexity of the global health context increases with persistent pandemics of infectious diseases and the rising prevalence of noncommunicable diseases, there is a growing appreciation that conditions selected as nutrition/health targets indeed represent syndromes for which nutritional status serves as both an input and outcome. In recognition of the impact of these combined challenges and the role of the multiple manifestations of malnutrition, we suggest an approach to nutritional assessment that is intended to improve the precision of context-specific, equitable approaches to health promotion, disease prevention, and treatment.
Theory of Food: Unravelling the Lifelong Impact of Childhood Dietary Habits on Adult Food Preferences across Different Diet Groups
Authors: Horovitz Omer
Published: January 2024

The study investigates the behavioral manifestations of the “Theory of Food” (ToF), a novel theoretical framework centered on the early development of food perceptions. The ToF posits that childhood experiences with food shape cognitive networks influencing adult dietary choices. Stemming from the “Theory of Mind,” the ToF hypothesizes that individuals construct an associative world of food images and representations mirroring the socio-cognitive world shaped by proper theory of mind development. The study, involving 249 healthy adults, employs the Cognitive Food Preference Questionnaire (CFPQ) and the Adult Food Preference Profile (AFPP) to explore the correlation between childhood and adult food preferences across diet groups (omnivores, vegetarians, and vegans). Results reveal robust correlations in omnivores, varied patterns in vegetarians, and mixed outcomes in vegans. Notably, omnivores show correlations in grains, fast food, dairy products, vegetables, meat, soft drinks, and snack consumption. Vegetarians exhibit correlations in grains, fast food, dairy products, vegetables, snacks, and, surprisingly, meat consumption. Vegans display correlations in grains, fast food, vegetables, and snacks. The study suggests that childhood dietary habits tend to influence adult food choices, offering insights for future research in the field of theory of food (ToF).
Moderate-Intensity and High-Intensity Interval Exercise Training Offer Equal Cardioprotection, with Different Mechanisms, during the Development of Type 2 Diabetes in Rats
Authors: D’Haese Sarah , Claes Lisa , de Laat Iris , Van Campenhout Sven , Deluyker Dorien , Heeren Ellen , Haesen Sibren , Lambrichts Ivo , Wouters Kristiaan , Schalkwijk Casper G. , Hansen Dominique , Eijnde BO , Bito Virginie
Published: January 2024

Endurance exercise training is a promising cardioprotective strategy in type 2 diabetes mellitus (T2DM), but the impact of its intensity is not clear. We aimed to investigate whether and how isocaloric moderate-intensity exercise training (MIT) and high-intensity interval exercise training (HIIT) could prevent the adverse cardiac remodeling and dysfunction that develop T2DM in rats. Male rats received a Western diet (WD) to induce T2DM and underwent a sedentary lifestyle (n = 7), MIT (n = 7) or HIIT (n = 8). Insulin resistance was defined as the HOMA-IR value. Cardiac function was assessed with left ventricular (LV) echocardiography and invasive hemodynamics. A qPCR and histology of LV tissue unraveled underlying mechanisms. We found that MIT and HIIT halted T2DM development compared to in sedentary WD rats (p < 0.05). Both interventions prevented increases in LV end-systolic pressure, wall thickness and interstitial collagen content (p < 0.05). In LV tissue, HIIT tended to upregulate the gene expression of an ROS-generating enzyme (NOX4), while both modalities increased proinflammatory macrophage markers and cytokines (CD86, TNF-α, IL-1β; p < 0.05). HIIT promoted antioxidant and dicarbonyl defense systems (SOD2, glyoxalase 1; p < 0.05) whereas MIT elevated anti-inflammatory macrophage marker expression (CD206, CD163; p < 0.01). We conclude that both MIT and HIIT limit WD-induced T2DM with diastolic dysfunction and pathological LV hypertrophy, possibly using different adaptive mechanisms.
Rice Bran Oil Improves Emphysema in Cigarette Smoke Extract-Induced Mice through Anti-Inflammatory and Antioxidative Effects
Authors: Kettawan Aikkarach , Ruangklai Sukpattaraporn , Rungruang Thanaporn , Thongam Julalux , Kettawan Aurawan Kringkasemsee , Nirmal Nilesh , Srisuma Sorachai
Published: January 2024

Lung inflammation and alveolar enlargement are the major pathological conditions of chronic obstructive pulmonary disease (COPD) patients. Rice bran oil (RBO), a natural anti-inflammatory and antioxidative agent, has been used for therapeutic purposes in several inflammatory diseases. This study aimed to investigate the anti-inflammatory and antioxidative effect of RBO on a cigarette smoke extract (CSE)-induced emphysema model in mice. The results indicated that CSE significantly induced airspace enlargement in mouse lung. Increased inflammatory cells, macrophage, and TNF-alpha levels in bronchoalveolar lavage fluid (BALF) were noticed in CSE-treated mice. RBO (low and high dose)-supplemented mice showed decreased total BALF inflammatory cell, macrophage, and neutrophil numbers and TNF-alpha levels (p < 0.05). Additionally, the administration of RBO decreased the mean linear alveolar intercept (MLI) in the CSE-treated group. Additionally, RBO treatment significantly increased the total antioxidant capacity in both mouse BALF and serum. However, RBO did not have an effect on the malondialdehyde (MDA) level. These findings suggested that RBO treatment ameliorates lung inflammation in a CSE-induced emphysema mice model through anti-inflammatory and antioxidant pathways. Therefore, the supplementation of RBO could be a new potential therapeutic to relieve the severity of COPD.
Biopolymeric Nanogel as a Drug Delivery System for Doxorubicin—Improved Drug Stability and Enhanced Antineoplastic Activity in Skin Cancer Cells
Authors: Radeva Lyubomira , Zaharieva Maya M. , Spassova Ivanka , Kovacheva Daniela , Pencheva-El Tibi Ivanka , Najdenski Hristo , Yoncheva Krassimira
Published: January 2024

In this study, doxorubicin was loaded in a chitosan–albumin nanogel with the aim of improving its stability and exploring the potential of the system in the treatment of skin cancer. Infrared spectroscopy and X-ray diffraction confirmed the encapsulation of the drug. Transmission electron microscopy revealed the spherical shape of the nanogel particles. The drug-loaded nanogel was characterized with a small diameter of 29 nm, narrow polydispersity (0.223) and positive zeta potential (+34 mV). The exposure of encapsulated doxorubicin to light (including UV irradiation and daylight) did not provoke any degradation, whereas the nonencapsulated drug was significantly degraded. In vitro studies on keratinocytes (HaCaT) and epidermoid squamous skin carcinoma cells (A-431) disclosed that the encapsulated doxorubicin was more cytotoxic on both cell lines than the pure drug was. More importantly, the cytotoxic concentration of encapsulated doxorubicin in carcinoma cells was approximately two times lower than that in keratinocytes, indicating that it would not affect them. Thus, the loading of doxorubicin into the developed chitosan–albumin nanogel definitely stabilized the drug against photodegradation and increased its antineoplastic effect on the skin cancer cell line.
Chirality and Rigidity in Triazole-Modified Peptidomimetics Interacting with Neuropilin-1
Authors: Fedorczyk Bartłomiej , Redkiewicz Patrycja , Matalińska Joanna , Piast Radosław , Kosson Piotr , Wieczorek Rafał
Published: January 2024

The interaction of Neuropilin-1 (NRP-1) with vascular endothelial growth factor (VEGF) has been shown to promote angiogenesis under physiological and pathological conditions. Angiogenesis around tumors is a major factor allowing for their growth and spread. Disrupting NRP-1/VEGF complex formation is thus a promising pathway for the development of new anticancer pharmaceuticals. A large body of work has been produced in the last two decades detailing the development of inhibitors of NRP-1/VEGF complex formation. Among those were peptide A7R and its smaller derivatives KXXR and K(Har)XXR. It has been previously reported that replacement of the XX backbone with triazole residues has a positive effect on the proteolytic stability of inhibitors. It has also been reported that a higher dihedral angle range restriction of the XX backbone has a positive effect on the activity of inhibitors. In this work, we have designed new triazole derivatives of K(Har)XXR inhibitors with substitution allowing for higher range restriction of the XX backbone. The obtained peptidomimetics have greater activity than their less restricted counterparts. One of the newly obtained structures has greater affinity than the reference peptide A7R.
A Comprehensive Immunocapture-LC-MS/MS Bioanalytical Approach in Support of a Biotherapeutic Ocular PK Study
Authors: Chen Lin-Zhi , Roos David , Philip Elsy , Werth Emily G. , Kostuk Stephanie , Yu Hongbin , Fuchs Holger
Published: January 2024

BI-X, a therapeutic protein under development for the treatment of human ocular disease via intravitreal administration, binds to its therapeutic targets and endogenous albumin in the vitreous humor. A monkey ocular pharmacokinetic (PK) study following BI-X administration was conducted to measure drug and albumin levels in plasma, the vitreous humor, the aqueous humor, and retina tissue at various timepoints post-dose. A comprehensive bioanalytical approach was implemented in support of this study. Five immunocapture-LC-MS/MS assays were developed and qualified for quantitating BI-X in different matrices, while ELISA was used for albumin measurement. Immunocapture at the protein or peptide level was evaluated to achieve adequate assay sensitivity. Drug and albumin assays were applied for the analysis of the monkey study samples.
Multivariate Approaches in Quantitative Structure–Property Relationships Study for the Photostability Assessment of 1,4-Dihydropyridine Derivatives
Authors: Chieffallo Martina , De Luca Michele , Grande Fedora , Occhiuzzi Maria Antonietta , Gündüz Miyase Gözde , Garofalo Antonio , Ioele Giuseppina
Published: January 2024

1,4-dihydropyridines (1,4-DHPs) are widely recognized as highly effective L-type calcium channel blockers with significant therapeutic benefits in the treatment of cardiovascular disorders. 1,4-DHPs can also target T-type calcium channels, making them promising drug candidates for neurological conditions. When exposed to light, all 1,4-DHPs tend to easily degrade, leading to an oxidation product derived from the aromatization of the dihydropyridine ring. Herein, the elaboration of a quantitative structure–property relationships (QSPR) model was carried out by correlating the light sensitivity of structurally different 1,4-DHPs with theoretical molecular descriptors. Photodegradation experiments were performed by exposing the drugs to a Xenon lamp following the ICH rules. The degradation was monitored by spectrophotometry, and experimental data were elaborated by Multivariate Curve Resolution (MCR) methodologies to assess the kinetic rates. The results were confirmed by the HPLC-DAD method. PaDEL-Descriptor software was used to calculate molecular descriptors and fingerprints related to the chemical structures. Seventeen of the 1875 molecular descriptors were selected and correlated to the photodegradation rate by means of the Ordinary Least Squares (OLS) algorithm. The chemometric model is useful to predict the photosensitivity of other 1,4-DHP derivatives with a very low relative error percentage of 5.03% and represents an effective tool to design new analogs characterized by higher photostability.
Doxorubicin Conjugated γ-Globulin Functionalised Gold Nanoparticles: A pH-Responsive Bioinspired Nanoconjugate Approach for Advanced Chemotherapeutics
Authors: Chauhan Gaurav , Chopra Vianni , Alvarado América García , Gómez Siono Jocelyn Alexandra , Madou Marc J. , Martinez-Chapa Sergio Omar , Kulkarni Manish M.
Published: January 2024

Developing successful nanomedicine hinges on regulating nanoparticle surface interactions within biological systems, particularly in intravenous nanotherapeutics. We harnessed the surface interactions of gold nanoparticles (AuNPs) with serum proteins, incorporating a γ-globulin (γG) hard surface corona and chemically conjugating Doxorubicin to create an innovative hybrid anticancer nanobioconjugate, Dox-γG-AuNPs. γG (with an isoelectric point of ~7.2) enhances cellular uptake and exhibits pH-sensitive behaviour, favouring targeted cancer cell drug delivery. In cell line studies, Dox-γG-AuNPs demonstrated a 10-fold higher cytotoxic potency compared to equivalent doxorubicin concentrations, with drug release favoured at pH 5.5 due to the γ-globulin corona’s inherent pH sensitivity. This bioinspired approach presents a novel strategy for designing hybrid anticancer therapeutics. Our study also explored the intricacies of the p53-mediated ROS pathway’s role in regulating cell fate, including apoptosis and necrosis, in response to these treatments. The pathway’s delicate balance of ROS emerged as a critical determinant, warranting further investigation to elucidate its mechanisms and implications. Overall, leveraging the robust γ-globulin protein corona on AuNPs enhances biostability in harsh serum conditions, augments anticancer potential within pH-sensitive environments, and opens promising avenues for bioinspired drug delivery and the design of novel anticancer hybrids with precise targeting capabilities.
Spontaneous Emission Studies for Blue and Green InGaN-Based Light-Emitting Diodes and Laser Diodes
Authors: Choi Dae-Choul , Kim Yoon Seok , Kim Kyoung-Bo , Lee Sung-Nam
Published: January 2024

We investigated the efficiency droop phenomenon in blue and green GaN-based light-emitting diodes (LEDs) and laser diodes (LDs), which poses a significant challenge in high-power LEDs and is characterized by a reduction in external quantum efficiency at higher injection currents. Utilizing identical epi-structures for blue and green LEDs and LDs, with variations only in indium composition, our experiments revealed a gradual blue shift in the emission wavelengths as the injection current increased. Notably, the blue LED demonstrated a smaller shift compared to the green LED. In addition, the full width at half maximum of emission spectra increased with increasing injection current density, indicative of efficiency droop. Significantly, LDs consistently exhibited lower junction temperatures despite operating at higher current densities. This is attributed to the enhanced heat dissipation capability of the ridge waveguide LD structure, which results in a narrower emission spectrum and reduced efficiency droop compared to mesa LED structures. These outcomes highlight the efficiency of the ridge waveguide LD structure in heat dissipation from the active layer, offering crucial insights for the advancement of high-power light-emitting devices.
Evaluation of Cell Rupture Techniques for the Extraction of Proteins from the Microalgae Tetradesmus obliquus
Authors: da Silva César Augusto Sodré , Sibaja Karen Vanessa Marimón , de Ramos Cizilio Sabrina , Miranda Júnior José Roberto , de Castro Santana Rejane , Martins Marcio Arêdes , de Oliveira Leite Maurício , de Oliveira Eduardo Basílio , Coimbra Jane Sélia dos Reis
Published: January 2024

The high protein content of several microalgal species makes them attractive and unconventional candidates for use in the food and pharmaceutical industries. Due to the robust cell walls of microalgae, cell rupture is necessary to improve the extraction of intracellular proteins. Thus, choosing a suitable cell-breaking treatment before protein extraction is a vital downstream processing step. Additionally, it is necessary to use an effective technique for monitoring and measuring the impact of rupture treatments on microalgal cell walls. In our study, Tetradesmus obliquus cells were disrupted using three different mechanical rupture methods: high-pressure homogenization (HPH), ultrasound (US), and ball milling (BM). The ruptured biomass cells were counted, and soluble proteins were extracted and quantified. The cell-counting technique did not detect any differences between intact and damaged cells after BM treatment because the dye (erythrosine B) did not permeate the microalgal biomass accurately. The US treatment promoted the highest yield of total protein extraction (19.95%), while the highest yields in the HPH and BM treatments were 15.68% and 14.11%, respectively. Since the cell breakage method affects protein extraction from microalgal biomass, protein release can be used as a central indicator of the degree of cell disruption.