bluuebunny/crossref_metadata_2025 huggingface dataset:
Predictive Value of a Combined Model Based on Pre-Treatment and Mid-Treatment MRI-Radiomics for Disease Progression or Death in Locally Advanced Nasopharyngeal Carcinoma
Authors: Kang Le , Niu Yulin , Huang Rui , Lin Stefan (YUJIE) , Tang Qianlong , Chen Ailin , Fan Yixin , Lang Jinyi , Yin Gang , Zhang Peng
Published: December 2021

Purpose

A combined model was established based on the MRI-radiomics of pre- and mid-treatment to assess the risk of disease progression or death in locally advanced nasopharyngeal carcinoma.

Materials and Methods

A total of 243 patients were analyzed. We extracted 10,400 radiomics features from the primary nasopharyngeal tumors and largest metastatic lymph nodes on the axial contrast-enhanced T1 weighted and T2 weighted in pre- and mid-treatment MRI, respectively. We used the SMOTE algorithm, center and scale and box-cox, Pearson correlation coefficient, and LASSO regression to construct the pre- and mid-treatment MRI-radiomics prediction model, respectively, and the risk scores named P score and M score were calculated. Finally, univariate and multivariate analyses were used for P score, M score, and clinical data to build the combined model and grouped the patients into two risk levels, namely, high and low.

Result

A combined model of pre- and mid-treatment MRI-radiomics successfully categorized patients into high- and low-risk groups. The log-rank test showed that the high- and low-risk groups had good prognostic performance in PFS (P<0.0001, HR: 19.71, 95% CI: 12.77–30.41), which was better than TNM stage (P=0.004, HR:1.913, 95% CI:1.250–2.926), and also had an excellent predictive effect in LRFS, DMFS, and OS.

Conclusion

Risk grouping of LA-NPC using a combined model of pre- and mid-treatment MRI-radiomics can better predict disease progression or death.
The ncRNA-Mediated Overexpression of Ferroptosis-Related Gene EMC2 Correlates With Poor Prognosis and Tumor Immune Infiltration in Breast Cancer
Authors: Liu Xing , Yang Pengshuo , Han Lu , Zhou Qing , Qu Qingsong , Shi Xinyuan
Published: December 2021

Ferroptosis is an iron-dependent programmed cell death process. Although ferroptosis inducers hold promising potential in the treatment of breast cancer, the specific role and mechanism of the ferroptosis-related gene EMC2 in breast cancer have not been entirely determined. The potential roles of EMC2 in different tumors were explored based on The Cancer Genome Atlas (TCGA), Genotype-Tissue Expression (GTEx), Gene Expression Profiling Interactive Analysis 2 (GEPIA2), Tumor Immune Estimation Resource (TIMER), Shiny Methylation Analysis Resource Tool (SMART), starBase, and cBioPortal for cancer genomics (cBioPortal) datasets. The expression difference, mutation, survival, pathological stage, DNA methylation, non-coding RNAs (ncRNAs), and immune cell infiltration related to EMC2 were analyzed. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were performed to identify the differences in biological processes and functions among different related genes. The expression levels of core prognostic genes were then verified in breast invasive carcinoma samples using immunohistochemistry and breast invasive carcinoma cell lines using real-time polymerase chain reaction. High expression levels of EMC2 were observed in most cancer types. EMC2 expression in breast cancer tissue samples correlated with poor overall survival. EMC2 was mutated and methylated in a variety of tumors and affected survival. The LINC00665-miR-410-3p axis was identified as the most potential upstream ncRNA-related pathway of EMC2 in breast cancer. EMC2 levels were significantly positively correlated with tumor immune cell infiltration, immune cell biomarkers, and immune checkpoint expression. Our study offers a comprehensive understanding of the oncogenic roles of EMC2 across different tumors. The upregulation of EMC2 expression mediated by ncRNAs is related to poor prognosis and tumor immune infiltration in breast cancer.
A Novel miR-98 Negatively Regulates the Resistance of Endometrial Cancer Cells to Paclitaxel by Suppressing ABCC10/MRP-7
Authors: Huang Wei , Zhang Jun , Dong Biao , Chen Haiting , Shao Liwei , Li Xiaohui
Published: December 2021

Endometrial cancer (EC) is one of the most frequent gynecological tumors, and chemoresistance is a major obstacle to improving the prognosis of EC patients. MicroRNAs (miRNAs) and long non-coding RNAs (lncRNAs) have recently emerged as crucial chemoresistance regulators that alter the levels of downstream target genes. Multidrug Resistance Protein 7 (MRP-7/ABCC10) is an ATP-binding cassette transporter that causes the resistance to anti-cancer drugs. The purpose of this research is to determine whether MRP-7 has a role in mediating the sensitivity of EC cells to paclitaxel and whether the expression of MRP-7 is regulated by miR-98 and lncRNA NEAT1. We reported that the levels of MRP-7 were significantly increased in EC tissues and associated with an unfavorable prognosis. Downregulation of MRP-7 in EC cells sensitized these cells to paclitaxel and reduced cell invasion. PLAUR serves as a downstream molecule of MRP-7 and facilitates paclitaxel resistance and EC cell invasiveness. Moreover, miR-98 serves as a tumor suppressor to inhibit MRP-7 expression, leading to the repression of paclitaxel resistance. Furthermore, a novel lncRNA, NEAT1, was identified as a suppressor of miR-98, and NEAT1 could upregulate MRP-7 levels by reducing the expression of miR-98. Taken together, these findings demonstrate that upregulation of MRP-7 and NEAT1, and downregulation of miR-98 have important roles in conferring paclitaxel resistance to EC cells. The modulation of these molecules may help overcome the chemoresistance against paclitaxel in EC cells.
Inhibition of Podocytes DPP4 Activity Is a Potential Mechanism of Lobeliae Chinensis Herba in Treating Diabetic Kidney Disease
Authors: Wang Xinyu , Xiang Jiaqing , Huang Guixiao , Kang Lin , Yang Guangyan , Wu Han , Jiang Kewei , Liang Zhen , Yang Shu
Published: December 2021

Diabetic kidney disease (DKD) is the leading cause of end-stage renal disease and has become a serious public health problem worldwide. Dipeptidyl peptidase-4 (DPP4) inhibitors, an emerging drug for the treatment of diabetes, have been found to have renoprotective effects in addition to glucose-lowering effects and therefore have the potential to be a treatment modality for DKD. Lobeliae Chinensis Herba (LCH), a traditional Chinese herb widely used in the treatment of diabetes, has recently been found to have a hypoglycaemic mechanism related to the inhibition of DPP4. Firstly, analysis of single-cell sequencing data from mouse kidneys in the National Center for Biotechnology Information (NCBI) database revealed that DPP4 was specifically upregulated in DKD podocytes and was associated with podocyte proliferation. Subsequently, the network pharmacology approach was applied to the screening of compounds. Twelve LCH active ingredients targeting DPP4 were extracted from the Traditional Chinese Medicine System Pharmacology (TCMSP) database. In addition, these 12 compounds and DPP4 were molecularly docked to predict the probability of them affecting DPP4 activity. In vitro, Quercetin, Methyl rosmarinate, Kaempferol, Diosmetin and Acacetin were demonstrated to retard podocyte proliferation by inhibiting DPP4 activity and were the top five compounds predicted by molecular docking to be the most likely to affect DPP4 activity. The half maximal inhibitory concentration (IC50) of the five compounds for DPP4 activity were as follows. Acacetin Log IC50 = −8.349, 95%CI (−9.266, −7.265), Diosmtrin Log IC50 = −8.419, 95%CI (−8.889, −7.950), Log IC50 = −8.349, 95%CI (−9.266, −7.265), Methyl rosmarinate Log IC50 = −8.415, 95%CI (−8.751, −8.085), Kaempferol Log IC50 = −8.297, 95%CI (−9.001, −7.615), Quercetin Log IC50 = −8.864, 95%CI (−9.107, −8.615). Finally, Quercetin, Methyl rosmarinate, Kaempferol, Diosmetin and Acacetin qualified for pharmacokinetic and drug similarity screening and have the potential to be the most promising oral agents for the treatment of DKD.
Parallel Processing of Olfactory and Mechanosensory Information in the Honey Bee Antennal Lobe
Authors: Tiraboschi Ettore , Leonardelli Luana , Segata Gianluca , Haase Albrecht
Published: December 2021

In insects, neuronal responses to clean air have so far been reported only episodically in moths. Here we present results obtained by fast two-photon calcium imaging in the honey bee Apis mellifera, indicating a substantial involvement of the antennal lobe, the first olfactory neuropil, in the processing of mechanical stimuli. Clean air pulses generate a complex pattern of glomerular activation that provides a code for stimulus intensity and dynamics with a similar level of stereotypy as observed for the olfactory code. Overlapping the air pulses with odor stimuli reveals a superposition of mechanosensory and odor response codes with high contrast. On the mechanosensitive signal, modulations were observed in the same frequency regime as the oscillatory motion of the antennae, suggesting a possible way to detect odorless airflow directions. The transduction of mechanosensory information via the insect antennae has so far been attributed primarily to Johnston’s organ in the pedicel of the antenna. The possibility that the antennal lobe activation by clean air originates from Johnston’s organ could be ruled out, as the signal is suppressed by covering the surfaces of the otherwise freely moving and bending antennae, which should leave Johnston’s organ unaffected. The tuning curves of individual glomeruli indicate increased sensitivity at low-frequency mechanical oscillations as produced by the abdominal motion in waggle dance communication, suggesting a further potential function of this mechanosensory code. The discovery that the olfactory system can sense both odors and mechanical stimuli has recently been made also in mammals. The results presented here give hope that studies on insects can make a fundamental contribution to the cross-taxa understanding of this dual function, as only a few thousand neurons are involved in their brains, all of which are accessible by in vivo optical imaging.
Phenomics-Assisted Selection for Herbage Accumulation in Alfalfa (Medicago sativa L.)
Authors: Biswas Anju , Andrade Mario Henrique Murad Leite , Acharya Janam P. , de Souza Cleber Lopes , Lopez Yolanda , de Assis Giselle , Shirbhate Shubham , Singh Aditya , Munoz Patricio , Rios Esteban F.
Published: December 2021

The application of remote sensing in plant breeding is becoming a routine method for fast and non-destructive high-throughput phenotyping (HTP) using unmanned aerial vehicles (UAVs) equipped with sensors. Alfalfa (Medicago sativa L.) is a perennial forage legume grown in more than 30 million hectares worldwide. Breeding alfalfa for herbage accumulation (HA) requires frequent and multiple phenotyping efforts, which is laborious and costly. The objective of this study was to assess the efficiency of UAV-based imagery and spatial analysis in the selection of alfalfa for HA. The alfalfa breeding population was composed of 145 full-sib and 34 half-sib families, and the experimental design was a row-column with augmented representation of controls. The experiment was established in November 2017, and HA was harvested four times between August 2018 and January 2019. A UAV equipped with a multispectral camera was used for HTP before each harvest. Four vegetation indices (VIs) were calculated from the UAV-based images: NDVI, NDRE, GNDVI, and GRVI. All VIs showed a high correlation with HA, and VIs predicted HA with moderate accuracy. HA and NDVI were used for further analyses to calculate the genetic parameters using linear mixed models. The spatial analysis had a significant effect in both dimensions (rows and columns) for HA and NDVI, resulting in improvements in the estimation of genetic parameters. Univariate models for NDVI and HA, and bivariate models, were fit to predict family performance for scenarios with various levels of HA data (simulated in silico by assigning missing values to full dataset). The bivariate models provided higher correlation among predicted values, higher coincidence for selection, and higher genetic gain even for scenarios with only 30% of HA data. Hence, HTP is a reliable and efficient method to aid alfalfa phenotyping to improve HA. Additionally, the use of spatial analysis can also improve the accuracy of selection in breeding trials.
Rice Na+-Permeable Transporter OsHAK12 Mediates Shoots Na+ Exclusion in Response to Salt Stress
Authors: Zhang Linan , Sun Xiangyu , Li Yanfang , Luo Xuan , Song Shaowen , Chen Yan , Wang Xiaohui , Mao Dandan , Chen Liangbi , Luan Sheng
Published: December 2021

Soil salinity has become a major stress factor that reduces crop productivity worldwide. Sodium (Na+) toxicity in a number of crop plants is tightly linked with shoot Na+ overaccumulation, thus Na+ exclusion from shoot is crucial for salt tolerance in crops. In this study, we identified a member of the high-affinity K+ transport family (HAK), OsHAK12, which mediates shoots Na+ exclusion in response to salt stress in rice. The Oshak12 mutants showed sensitivity to salt toxicity and accumulated more Na+ in the xylem sap, leading to excessive Na+ in the shoots and less Na+ in the roots. Unlike typical HAK family transporters that transport K+, OsHAK12 is a Na+-permeable plasma membrane transporter. In addition, OsHAK12 was strongly expressed in the root vascular tissues and induced by salt stress. These findings indicate that OsHAK12 mediates Na+ exclusion from shoot, possibly by retrieving Na+ from xylem vessel thereby reducing Na+ content in the shoots. These findings provide a unique function of a rice HAK family member and provide a potential target gene for improving salt tolerance of rice.
Systematic Characterization of MicroRNA Processing Modes in Plants With Parallel Amplification of RNA Ends
Authors: Li Ning , Ren Guodong
Published: December 2021

In plants, the RNase III-type enzyme Dicer-like 1 (DCL1) processes most microRNAs (miRNAs) from their primary transcripts called pri-miRNAs. Four distinct processing modes (i.e., short base to loop, sequential base to loop, short loop to base, and sequential loop to base) have been characterized in Arabidopsis, mainly by the Specific Parallel Amplification of RNA Ends (SPARE) approach. However, SPARE is a targeted cloning method which requires optimization of cloning efficiency and specificity for each target. PARE (Parallel Amplification of RNA Ends) is an untargeted method per se and is widely used to identify miRNA mediated target slicing events. A major concern with PARE in characterizing miRNA processing modes is the potential contamination of mature miRNAs. Here, we provide a method to estimate miRNA contamination levels and showed that most publicly available PARE libraries have negligible miRNA contamination. Both the numbers and processing modes detected by PARE were similar to those identified by SPARE in Arabidopsis. PARE also determined the processing modes of 36 Arabidopsis miRNAs that were unexplored by SPARE, suggesting that it can complement the SPARE approach. Using publicly available PARE datasets, we identified the processing modes of 36, 91, 90, and 54 miRNAs in maize, rice, soybean, and tomato, respectively, and demonstrated that the processing mode was conserved overall within each miRNA family. Through its power of tracking miRNA processing remnants, PARE also facilitated miRNA characterization and annotation.
Distinct Responses of Leaf Traits to Environment and Phylogeny Between Herbaceous and Woody Angiosperm Species in China
Authors: An Nannan , Lu Nan , Fu Bojie , Wang Mengyu , He Nianpeng
Published: December 2021

Leaf traits play key roles in plant resource acquisition and ecosystem processes; however, whether the effects of environment and phylogeny on leaf traits differ between herbaceous and woody species remains unclear. To address this, in this study, we collected data for five key leaf traits from 1,819 angiosperm species across 530 sites in China. The leaf traits included specific leaf area, leaf dry matter content, leaf area, leaf N concentration, and leaf P concentration, all of which are closely related to trade-offs between resource uptake and leaf construction. We quantified the relative contributions of environment variables and phylogeny to leaf trait variation for all species, as well as for herbaceous and woody species separately. We found that environmental factors explained most of the variation (44.4–65.5%) in leaf traits (compared with 3.9–23.3% for phylogeny). Climate variability and seasonality variables, in particular, mean temperature of the warmest and coldest seasons of a year (MTWM/MTWQ and MTCM/MTCQ) and mean precipitation in the wettest and driest seasons of a year (MPWM/MPWQ and MPDM/MPDQ), were more important drivers of leaf trait variation than mean annual temperature (MAT) and mean annual precipitation (MAP). Furthermore, the responses of leaf traits to environment variables and phylogeny differed between herbaceous and woody species. Our study demonstrated the different effects of environment variables and phylogeny on leaf traits among different plant growth forms, which is expected to advance the understanding of plant adaptive strategies and trait evolution under different environmental conditions.
The Development and Validation of the Doctoral Student Identity Scale
Authors: Zhao Jia-lu , Chen Fu , Jia Xiao-ming
Published: December 2021

Objective:Based on how the identity of doctoral students is recognized and understood in the context of Chinese culture, we developed a doctoral identity scale using both qualitative and quantitative analyses.

Methods:The initial project of the Scale was formed through qualitative analyses and expert consultation. Nine hundred and ninety-one doctoral students were officially tested, and 982 valid questionnaires were obtained. They were randomly divided into two parts, and 491 of which were assessed for item Response Theory (IRT) and exploratory factor analysis (EFA) and 491 of which were assessed for confirmatory factor analysis (CFA). The Subjective Well-Being Scale (SWB), the Rosenberg Self-Esteem Scale (RSE), and the Psychological Sense of School Membership Scale (PSSM) were used to test its the criterion-related validity. One hundred and forty-one students were selected for retesting after 8 weeks.

Results:The doctoral student identity questionnaire consisted of two factors identity exploration and identity commitment, explaining 57% of the total variance. The results of CFA showed that the two-factor model fitted the data well. The two dimensions of the Doctoral Student Identity Scale were significantly and positively correlated with the two dimensions of the SWB scale (0.32–0.66), the latent factor of the RSE scale (0.42–0.55), and the latent factor of the PSSM scale (0.52–0.62). Composite reliability values for exploration and commitment were 0.79 and 0.83 respectively, and the values of McDonald’s omega for exploration and commitment were 0.81 and 0.85 respectively. The test-retest reliability of the total questionnaire was 0.842.

Conclusion:The Doctoral Student Identity Scale was developed with good reliability and validity, and can be used as a reliable tool for measuring the doctoral student identity. In addition, the questionnaire will provide corresponding ideas and methods for studying the identity issues of specific groups.
Case Report: Catatonia Associated With Post-traumatic Stress Disorder
Authors: Ahmed Gellan K. , Elbeh Khaled , Karim Ahmed A. , Khedr Eman M.
Published: December 2021

We report here about a 12-year-old female patient who had two life-threatening accidents that led to post-traumatic stress disorder associated with catatonia. She had closed eyes, had urinary and fecal incontinence, and had been in an abnormal position for one and half month. Moreover, she had complications such as dehydration, malunion of the fractured arm, and deformities in hand and foot. After detailed psychiatric examination, neurological assessment, and laboratory investigation, the patient received successful treatment in the form of benzodiazepine injections, intravenous fluid, oral antidepressants, and six sessions of electroconvulsive therapy (ECT). We discuss the pathophysiology of catatonia, which remains elusive, and recommend evaluating catatonic children for any possible trauma during psychiatry assessment.
Enhancement of Social Communication Behaviors in Young Children With Autism Affects Maternal Stress
Authors: Laister Dominik , Vivanti Giacomo , Marschik Peter B. , Fellinger Johannes , Holzinger Daniel
Published: December 2021

Children with autism spectrum disorder (ASD) show difficulties in social communication behaviors, emotion regulation and daily living skills, and they frequently present with challenging behaviors. In parents of children with ASD, higher rates of stress and mental health problems have been reported than in parents of either typically developing children or children with other conditions. In this study, we tested whether maternal well-being changes with improved social communicative behaviors of children with ASD receiving early intervention. We examined developmental changes in 72 pre-schoolers and stress levels in their mothers (measured by the Parental Stress Inventory) before and after a 12-month community-based intervention program based on the Early Start Denver Model, a naturalistic developmental behavioral intervention targeting social communication. Multiple regression analyses showed that maternal child-related stress was predicted by changes in children's social communication behaviors (measured with the Pervasive Developmental Disorder Behavior Inventory). Gains in the early social communication behavior domain were the strongest predictor of post-intervention child-related maternal stress, surpassing adaptive behavior, language and non-verbal cognitive gains, and reduction in challenging behavior. These findings support the hypothesis that, in children with ASD, the acquisition of social communication behaviors contribute to improvements in maternal well-being.
Location, Clinical Presentation, Diagnostic Algorithm and Open vs. Arthroscopic Surgery of Knee Synovial Haemangioma: A Report of Four Cases and a Literature Review
Authors: Hernández-Hermoso José A. , Moranas-Barrero José , García-Oltra Ester , Collado-Saenz Fernando , López-Marne Sylvia
Published: December 2021

Objective: The aim was to report 4 patients with intra-articular knee synovial haemangioma (KSH) and to perform a systematic review to describe the patient characteristics, patterns of tumor location, clinical presentation, usefulness of imaging examinations, pros and cons of arthroscopic vs. open resection, and follow-up in the literature.

Design: From 1996 to 2016, four patients with KSH were retrospectively reviewed. A literature search was conducted in PubMed from 2000/01 to 2020/06 using the search terms “synovial haemangioma” and “knee.” Fifty full-text articles that included a total of 92 patients were included for further discussion.

Results: Four adults (20–40 years) were diagnosed with KSH. Three lesions located in the suprapatellar pouch, two eroding the patella and one the supratrochlear bone, and one in the posterior compartment. Persistent anterior knee pain was the main complain. MRI revealed a benign tumor mass in all cases except one. Open excisional biopsy and regional synovectomy were performed in three patients, and by arthroscopy of the posterior compartment in the fourth. Histological type was arteriovenous in three cases and capillary in one. A pain-free knee without recurrence was achieve in all cases except one, which was successfully reoperated. Average follow-up time was 3.5 years. A literature review showed that KSH appears most frequently in children and teenagers (64.6%) and does not differ by gender. The suprapatellar and patella-femoral joint compartment was the most frequent location (47.9%). The bony tissue of the knee was rarely affected (13.5%). Pain, swelling and haemarthrosis were frequently reported (88.2, 66.7, and 47.1%). MRI was the most commonly used imaging test (98%). Treatment consisted of regional synovectomy by open surgery or arthroscopy in 66.7 and 15.6% of cases, respectively.

Conclusions: KSH should be considered in the differential diagnosis of adult patients with chronic low-intensity knee pain. MRI is the most useful exam because it establishes the location, extent and benign characteristics of the tumor. Definitive diagnosis requires histological examination. We believe excisional biopsy and regional synovectomy by arthroscopy should be the treatments of choice for intra-articular tumors, but we recommend open surgery when the lesion extends to the tendons, muscle or bone.
Risk Factors in Various Climates of Wheat Production in Western Iran: Experts’ Opinions
Authors: Ranjbar Zahra , Chizari Mohammad , Sadighi Hasan , Farhadian Homayon , Lebailly Philippe , Dogot Thomas , Ortegón Rojas Jorge Armando , Parra-Acosta Yenny Katherine , Azadi Hossein
Published: December 2021

Agriculture is the origin of existence and survival in any society. However, this sector is always associated with risk and uncertainty, and farmers are faced with many challenges. Therefore, the main aim of this research was to explore the (production) risk factors of irrigated wheat production systems among farmers in Kermanshah province, Iran. The exploratory nature of this study on the one hand and the need for expert judgment on the other hand necessitated a Delphi research design. Thus, this study utilized a three-round Delphi technique. The population comprised of 10 subject-matter experts (SME) and 32 agricultural extension experts in two climates (warm and semi-arid and cold and moderate) in seven counties in Kermanshah province. A purposeful (complete) sample of 42 experts in the agricultural field participated in this study. Data were collected using a questionnaire designed on the basis of the Delphi technique. The findings indicated that, after three rounds of Delphi technique, the panel members reached a consensus on 75 factors which were categorized into biological, natural, managerial, and technological-structural groups for production risk. The results of this research provide useful insights for researchers, extension agents, and farmers.
Substrate-Dependent Effect of Vermicompost on Yield and Physiological Indices of Container-Grown Dracocephalum moldavica Plants
Authors: Ose Alise , Andersone-Ozola Una , Ievinsh Gederts
Published: December 2021

The development of sustainable plant production systems involves a search for different alternatives to chemical fertilizers. The aim of the present study is to compare growth and physiological effects of vermicompost on Dracocephalum moldavica plants in controlled conditions, using two types of commercially available substrates. The intention is to determine whether nondestructively measured photosynthesis-related parameters are useful for monitoring the physiological status of plants. The plants were cultivated in two base substrates without or with the addition of mineral fertilizer, as well as an amendment with vermicompost at a 20% or 30% rate in the conditions of an automated greenhouse. The biomass accumulation for control plants of D. moldavica was identical in peat substrate and commercial garden soil. The average growth increase by mineral fertilizer was 25% for D. moldavica plants grown in peat and 15% for plants grown in soil. Substrate amendment with 20% vermicompost resulted in an 114% average increase in biomass for plants grown in peat and a 98% average increase for plants grown in soil, but for plants at 30% the amendment rate increase was 148% and 68%, for peat and soil, respectively. Consequently, the addition of an identical amount of vermicompost resulted in a poorer growth response of plants in commercial garden soil as a substrate in comparison to peat, but an increase in the amendment rate from 20% to 30% resulted in some growth inhibition for these plants. Chlorophyll concentration was positively affected by the vermicompost amendment in a concentration-dependent manner, but this effect during a cultivation period appeared relatively late. Large differences were found between the three groups of fluorescence-derived parameters, with variable levels of predictability with respect to the differences in plant yield due to the pronounced variation in correlation through time. It is concluded that the incorporation of vermicompost for the cultivation of D. moldavica, even in substrate mixes with relatively high and balanced composition of plant-available nutrients, benefits plant growth, physiological status and biomass yield, but it is necessary to explore interactions between vermicompost and other substrates leading to possible changes in quality-related characteristics of vermicompost in substrate mixes.
Potentially Toxic Elements in Oasis Agricultural Soils Caused by High-Intensity Exploitation in the Piedmont Zone of the Tianshan Mountains, China
Authors: Liu Wen , Ma Long , Abuduwaili Jilili
Published: December 2021

Considering the pollution of potentially toxic elements (PTEs) in the soils of China, the present study analyzed the current state and influencing factors of PTEs in oasis soils using the model of absolute principal component score–multiple linear regression in the piedmont zone of the Tianshan Mountains. The possible non-carcinogenic and carcinogenic risks of PTEs at current concentrations were also explored using a human-health risk-assessment model. The results suggested that the extent to which potentially toxic elements in the soils of different geographical units in the study area is affected by human activities varies considerably. The PTEs Cd and As in the soils of the Yili River Watershed were the most strongly influenced by human activities, reaching levels of 40% and 59%, respectively. However, in the Bortala River Watershed, Cu, Cd, and As were the most strongly influenced by human activities, reaching levels of 33%, 64%, and 76%, respectively. Geographical units with a high degree of economic development (e.g., the Yili River Watershed) had, in contrast, low levels of PTE pollution caused by human activities, which may be related to the regional economic development structure. The human health risk assessment showed that the non-carcinogenic and carcinogenic risks of PTEs are currently below the threshold. However, increasing the arsenic content to 1.78 times the current level in the Bortala River Watershed would lead to carcinogenic risk. For the Yili River Watershed, a 3.33-fold increase in the arsenic content above its current level would lead to a carcinogenic risk. This risk should be addressed, and targeted environmental-protection measures should be formulated. The present research results will provide important decision support for regional environmental protection.
Lead-Tolerant Bacillus Strains Promote Growth and Antioxidant Activities of Spinach (Spinacia oleracea) Treated with Sewage Water
Authors: Najm-ul-Seher  , Ahmad Maqshoof , Ahmad Iqra , Nazli Farheen , Mumtaz Muhammad Zahid , Latif Muhammad , Al-Mosallam Mobarak S. , Alotaibi Fahad S. , Dewidar Ahmed Z. , Mattar Mohamed A. , El-Shafei Ahmed A.
Published: December 2021

Irrigation with sewage-contaminated water poses a serious threat to food security, particularly in developing countries. Heavy metal tolerant bacteria are sustainable alternatives for the removal of wastewater contaminants. In the present study, four lead (Pb)-tolerant strains viz. Bacillus megaterium (N8), Bacillus safensis (N11), Bacillus sp. (N18), and Bacillus megaterium (N29) were inoculated in spinach and grown in sewage water treated earthen pots separately and in combination with canal water. Results showed that Pb-tolerant strains significantly improved plant growth and antioxidant activities in spinach and reduces metal concentration in roots and leaves of spinach plants irrigated with treated wastewater. Strain Bacillus sp. (N18) followed by B. safensis (N11) caused the maximum increase in shoot length, root length, shoot fresh weight, root fresh weight, shoot dry weight, root dry weight, and leaf area compared to the uninoculated control of sewage water treated plants. These strains also improved antioxidant enzymatic activity including catalase, guaiacol peroxidase dismutase, superoxide dismutase, and peroxidases activities compared to the uninoculated control under sewage water conditions. Strain Bacillus sp. (N18) followed by B. safensis (N11) showed the highest reduction in nickel, cadmium, chromium, and Pb contents in roots and leaves of spinach compared to the uninoculated control plants treated with the sewage water. Such potential Pb-tolerant Bacillus strains could be recommended for the growth promotion of spinach after extensive evaluation under field conditions contaminated with wastewater.
Are We Approaching Peak Meat Consumption? Analysis of Meat Consumption from 2000 to 2019 in 35 Countries and Its Relationship to Gross Domestic Product
Authors: Whitton Clare , Bogueva Diana , Marinova Dora , Phillips Clive J. C.
Published: December 2021

Growing prosperity, but also disease outbreaks, natural disasters, and consumer preferences are changing global meat consumption. We investigated the 2000–2019 trends in 35 countries monitored by the Food and Agriculture Organization and the Organisation for Economic Co-operation and Development. We also tested relationships with Gross Domestic Product (GDP). Several countries appeared to be reaching peak consumption of some meats, and three (New Zealand, Canada, and Switzerland) have reached this. Poultry consumption increased over time in most countries, and beef and mutton/lamb consumption decreased in many. Using cluster analysis, we divided countries into two clusters: one in which increases in GDP per capita matched increases in meat consumption; and a second one of nine countries, for which there was no association between per capita change in GDP and meat consumption. There was evidence of a tipping point around USD 40,000 of GDP per capita, after which increases in economic well-being do not lead to increased meat consumption.
Influence of the Season and Region Factor on Phosphoproteome of Stallion Epididymal Sperm
Authors: Dyrda Katarzyna , Orzołek Aleksandra , Ner-Kluza Joanna , Wysocki Paweł
Published: December 2021

Epididymal maturation can be defined as a scope of changes occurring during epididymal transit that prepare spermatozoa to undergo capacitation. One of the most common post-translational modifications involved in the sperm maturation process and their ability to fertilise an oocyte is the phosphorylation of sperm proteins. The aim of this study was to compare tyrosine, serine, and threonine phosphorylation patterns of sperm proteins isolated from three subsequent segments of the stallion epididymis, during and out of the breeding season. Intensities of phosphorylation signals and phosphoproteins profiles varied in consecutive regions of the epididymis. However, significant differences in the phosphorylation status were demonstrated in case of endoplasmic reticulum chaperone BiP (75 and 32 kDa), protein disulfide-isomerase A3 (50 kDa), nesprin-1 (23 kDa), peroxiredoxin-5 (17 kDa), and protein bicaudal D homolog (15 kDa) for season x type of phosphorylated residues variables. Significant differences in the phosphorylation status were also demonstrated in case of endoplasmic reticulum chaperone BiP and albumin (61 kDa), protein disulfide-isomerase A3 (50 kDa), and protein bicaudal D homolog (15 kDa) for region x type of phosphorylated residues variables.
Treating Bacterial Infections with Bacteriophage-Based Enzybiotics: In Vitro, In Vivo and Clinical Application
Authors: Danis-Wlodarczyk Katarzyna M. , Wozniak Daniel J. , Abedon Stephen T.
Published: December 2021

Over the past few decades, we have witnessed a surge around the world in the emergence of antibiotic-resistant bacteria. This global health threat arose mainly due to the overuse and misuse of antibiotics as well as a relative lack of new drug classes in development pipelines. Innovative antibacterial therapeutics and strategies are, therefore, in grave need. For the last twenty years, antimicrobial enzymes encoded by bacteriophages, viruses that can lyse and kill bacteria, have gained tremendous interest. There are two classes of these phage-derived enzymes, referred to also as enzybiotics: peptidoglycan hydrolases (lysins), which degrade the bacterial peptidoglycan layer, and polysaccharide depolymerases, which target extracellular or surface polysaccharides, i.e., bacterial capsules, slime layers, biofilm matrix, or lipopolysaccharides. Their features include distinctive modes of action, high efficiency, pathogen specificity, diversity in structure and activity, low possibility of bacterial resistance development, and no observed cross-resistance with currently used antibiotics. Additionally, and unlike antibiotics, enzybiotics can target metabolically inactive persister cells. These phage-derived enzymes have been tested in various animal models to combat both Gram-positive and Gram-negative bacteria, and in recent years peptidoglycan hydrolases have entered clinical trials. Here, we review the testing and clinical use of these enzymes.
Prevention Effect of TGF-β Type I Receptor Kinase Inhibitor in Esophageal Stricture Formation after Corrosive Burn
Authors: Kim Min-Tae , Kim Kun-Yung
Published: December 2021

Corrosive burns lead to progressive esophageal stricture and dysphagia. There are many trials to prevent esophageal stricture formation after corrosive burn. EW-7197 has been proven in several animal models of fibrosis to have antifibrotic and antiproliferative effect. This study aimed to assess the effects of EW-7197 on prevention for esophageal stricture formation after corrosive esophageal burn. An animal study was carried out, where the animals were divided into three groups: a healthy group, a control group (corrosive burn without EW-7197), and a treatment group (corrosive burn with EW-7197). Corrosive esophageal burns were induced using 30% NaOH on the lower esophagus. For 3 weeks, the control group received vehicle and the treatment group received 20 mg/kg/day EW-7197. Treatment efficacy was assessed by measuring the stenosis ratio by esophagogram with contrast media on day 21. Histologic staining was performed to evaluate the fibrosis area ratio, and Western blotting was performed to evaluate fibrotic markers. Among 20 rats that underwent surgery, 14 survived. Three in the treatment group died because of esophageal perforation, and three in the control group died due to their debilitating status. The esophageal stenosis ratio was significantly lower in the treatment group than in the control group (12.1 ± 9.5% and 42.2 ± 8.3%, respectively; p = 0.001). The histologic fibrosis area ratio was also significantly lower in the treatment group (12.5 ± 3.0% and 21.6 ± 2.1%, respectively; p = 0.001). The treatment group showed lower expressions of profibrogenic proteins such as TGF-β1, pSmad3, and α-SMA. EW-7197 may be a good alternative for the prevention esophageal stricture formation after corrosive burn.
Transcranial Direct Current Stimulation (tDCS) Combined with Therapeutic Exercise and Cognitive Rehabilitation to Treat a Case of Burning Mouth Syndrome (BMS) Related Pain
Authors: Sánchez-Cuesta Francisco José , González-Zamorano Yeray , Arroyo-Ferrer Aida , Avellanal Martín , Fernández-Carnero Josué , Romero Juan Pablo
Published: December 2021

Burning Mouth Syndrome (BMS) is a multifactorial, chronic pain condition with neuropathic and psychogenic mechanisms. Cortical activation as well as sustained attention and executive functions have proven to be affected by chronic pain. The main objectives of this work were to test the efficacy of a multidimensional personalized pain treatment protocol and to investigate if the effects are based on psychophysical pain processing changes or cognitive effects. A 74-year-old female with 2 years of BMS received 10 sessions of a combined protocol of anodal left dorsolateral prefrontal cortex tDCS, cognitive therapy, and therapeutic exercise. The subjective perception of pain decreased by 47% after treatment but returned to the baseline at 45 days. No changes were found in objective pain measurements apart from a transient worsening of conditioned pain modulation. A large effect size was found in all functional scales, processing speed and executive control as well as sustained attention that persisted during follow-up. No changes in anxiety and depression were found. A multimodal therapeutic approach combining TDCS, cognitive rehabilitation and therapeutic exercise produces improved quality of life, disability and pain perception correlated with improvements in processing speed, executive control and sustained attention but independent of changes in psychophysical pain processing.
An Air Spring Vibration Isolator Based on a Negative-Stiffness Structure for Vehicle Seat
Authors: Nguyen Cong Hung , Ho Cong Minh , Ahn Kyoung Kwan
Published: December 2021

This research introduces an air spring vibration isolator system (ASVIS) based on a negative-stiffness structure (NSS) to improve the vehicle seat’s vibration isolation performance at low excitation frequencies. The main feature of the ASVIS consists of two symmetric bellows-type air springs which were designed on the basis of a negative stiffness mechanism. In addition, a crisscross structure with two straight bars was also used as the supporting legs to provide the nonlinear characteristics with NSS. Moreover, instead of using a vertical mechanical spring, a sleeve-type air spring was employed to provide positive stiffness. As a result, as the weight of the driver varies, the dynamic stiffness of the ASVIS can be easily adjusted and controlled. Next, the effects of the dimension parameters on the nonlinear force and nonlinear stiffness of ASVIS were analyzed. A design process for the ASVIS is provided based on the analytical results in order to achieve high static–low dynamic stiffness. Finally, numerical simulations were performed to evaluate the effectiveness of the ASVIS. The results obtained in this paper show that the values of the seat displacement of the ASVIS with NSS were reduced by 77.16% in comparison with those obtained with the traditional air spring isolator without NSS, which indicates that the design of the ASVIS isolator with NSS allows the effective isolation of vibrations in the low-frequency region.
The Pattern of Affective Responses to Dance-Based Group Exercise Differs According to Physical Fitness, as Measured by a Smartwatch
Authors: Kim Yujin , Kim Jihye , Woo Minjung
Published: December 2021

The present study investigated the effect of a dance-based aerobic exercise, on the affective experiences of participants with different fitness levels. Thirty-two college students were enrolled in the same dance fitness course, tested using a physical fitness test (the National Fitness Project 100) and grouped accordingly to 15 sports majors (high-fit group) and 17 non-sports majors (low-fit group). Together, they participated in a single-session dance fitness program using 11 basic dance steps incorporated in Zumba rhythms of merengue and reggaeton for 47 min including warm-up and cool-down. Pre- and post-exercise affects were measured using the PANAS-X transmitted to each participant’s smartphone. During exercise, participants’ heart rate (HR) and their responses to the felt arousal scale (FAS) and the feeling scale (FS) by exercise section were measured using tailor-made applications on a smartwatch. Results showed that the intensity of exercise for the same exercise program was lower in the high-fit group than in the low-fit group, as evidenced by %HRmax. In addition, the pattern of affective change throughout the exercise sections was different according to the groups’ fitness levels, while the affective improvement was greater in the high-fit group. This study confirmed that physical fitness is a major variable influencing the relationship between exercise and affect.
Experimental and Numerical Study of Thermal Performance of an Innovative Waste Heat Recovery System
Authors: Vizitiu Robert Stefan , Burlacu Andrei , Abid Cherifa , Verdes Marina , Balan Marius Costel , Branoaea Marius
Published: December 2021

One of the biggest challenges the world is facing these days is to reduce the greenhouse gases emissions in order to prevent the global warming. Since a significant quantity of CO2 emissions is the result of the energy producing process required in industry or buildings, the waste heat recovery is an important aspect in the fight for preserving the planet. In this study, an innovative waste heat recovery system which can recover waste heat energy from cooling liquids used in industry or in different processes, was designed and subjected to experimental investigations. The equipment uses heat pipes to capture thermal energy from the residual fluids transiting the evaporator zone and transfer it to the cold water transiting the condenser zone. The efficiency of the heat exchanger was tested in 9 scenarios, by varying the temperature of the primary agent to 60, 65 and 70 °C and the volume flow rate of the secondary agent to 1, 2 and 3 L/min. The temperature of the secondary agent and the volume flow rate of the primary agent were kept constant at 10 °C, respectively 24 L/min. The results were later validated through numerical simulations, and confirmed that the equipment can easily recover waste thermal energy from used water with low and medium temperatures at very low costs compared to the traditional heat exchangers. The results were promising, revealing an efficiency of the equipment up to 76.7%.
Analysis of Online Social Interactions Based on Positive Reinforcement Social Networks in a K–12 Geometry Class
Authors: Romero Sebastian , Fardoun Habib M. , Penichet Victor M. R. , Lozano Maria D. , Gallud Jose A.
Published: December 2021

Online social networks are part of our daily life. They are widely used among university students. These platforms have positive and negative aspects. Focusing on the positive aspects, positive reinforcement social networks (PRSNs) have been used to improve the participation of high school students in the classroom. The main objective of this study is to analyze online social interaction on PRSNs, in order to find out if they have a positive impact on classroom participation and students’ self-perceptions of their own social skills, especially in the supposedly difficult STEM (Science, Technology, Engineering and Mathematics) subjects, where engagement tends to be lower. An education-oriented PRSN platform has been developed and used by the experimental group of students to promote discussion and facilitate the sharing of different types of materials. These materials had to be related to students’ academic and professional life, but not necessarily related to Mathematics. A total of 72 Geometry students (K–12) and their teacher participated in a semester-long experimental study. Analyses of the interactions through the proposed platform combined with classroom participation revealed that students from the experimental group showed an improvement not found in the control group. Moreover, perceptions of students’ own online skills were analyzed, showing a positive impact after the experiment.