bluuebunny/crossref_metadata_2025 huggingface dataset:
Temporal Progression of Entry Factors into the Vicious Circle of Dry Eye in Untreated Sufferers
Authors: Garcia-Queiruga Jacobo , Pena-Verdeal Hugo , Sabucedo-Villamarin Belen , Garcia-Resua Carlos , Giraldez Maria J. , Yebra-Pimentel Eva
Published: June 2024

Background: Dry eye disease (DED) is characterized by the loss of ocular surface homeostasis with specific signs and symptoms. Studying the progression of a multifactorial disease is exceedingly challenging for researchers because several factors can influence it. The present study aims to study changes in tear meniscus height (TMH), lipid layer pattern (LLP), and bulbar hyperemia over time in untreated DED participants. Methods: This retrospective longitudinal study included 73 participants (146 eyes) diagnosed with DED since at least 2013. Participants underwent new examinations between 2021 and 2023, grouped by 8-, 6-, or 4-year follow-up periods. TMH, LLP, and bulbar hyperemia were assessed in both examinations. No participant received pharmacological treatment for DED. Results: Differences in TMH, bulbar hyperemia, and LLP between sessions were obtained in the 8-year group (p ≤ 0.027). Differences in bulbar hyperemia and LLP between sessions were obtained in the 6-year group (p ≤ 0.022). The only differences in LLP between sessions were obtained in the 4-year group (p < 0.005). Conclusion: Changes in TMH were obtained after periods of eight years from the first eye examination. Also, changes in bulbar hyperemia were obtained at periods of 8 and 6 years; however, changes in LLP could be found from 4-year follow-ups.
Unraveling the Genomic Evolution of Dengue Virus Serotype 1: A Case Study from Yantai, China
Authors: Sun Yulou , He Liqun , Li Xia , Li Cong , Yan Shicui , Zhang Yi , Sun Zhenlu
Published: June 2024

In August 2023, we identified a case of dengue fever in Yantai City, which was imported from Xishuangbanna, China. To investigate its evolutionary history and population dynamics, we utilized the metatranscriptomic method to obtain the virus’ whole genome sequence. Together with 367 selected dengue virus whole genome sequences from the NCBI database, we constructed a time-scaled Maximum Clade Credibility (MCC) tree. We found that our sequence exhibited a high homology with a sequence of DENV1 (OR418422.1) uploaded by the Guangzhou Center for Disease Control and Prevention in 2023, with an estimated divergence time around 2019 (95% HPD: 2017–2023), coinciding with the emergence of SARS-CoV-2. The DENV strain obtained in this study belongs to genotype I of DENV1. Its ancestors experienced a global epidemic around 2005 (95% HPD: 2002–2010), and its progeny strains have spread extensively in Southeast Asia and China since around 2007 (95% HPD: 2006–2011). The Bayesian skyline plot indicates that the current population of DENV1 has not been affected by SARS-CoV-2 and is expected to maintain stable transmission. Hence, it is imperative to track and monitor its epidemiological trends and genomic variations to prevent potential large-scale outbreaks in the post-SARS-CoV-2 era.
Familial Resemblance of Bone Health in Maternal Lineage Pairs and Triads: A Scoping Review
Authors: Boisvert Nicole M. J. , McElroy Melissa R. , Hayden K. Alix , Doyle-Baker Patricia K.
Published: June 2024

Introduction: Female bone health is influenced by familial resemblance, health parameters and maturational periods (puberty and menopause); this combination has been researched using familial multi-generational cross-sectional studies. Aim: This scoping review aimed to compile bone health research which uses sexually mature (grandmother–) mother–daughter pairs (and triads) and to determine the trends in its methodologies and familial comparisons. Methods: The Joanna Briggs Institute methodology for scoping reviews was used. Extraction included study and population characteristics, methodology (with an emphasis on imaging) and family-based results. Results: Twenty-nine studies were included, and their generations were categorized into four developmental categories: late adolescent to young adult, pre-menopause, mixed-menopause, and post-menopause. Eleven different pair/triad combinations were observed; the most common was pre-menopausal daughters and post-menopausal mothers. Dual-energy X-ray absorptiometry (DXA) was the most utilized imaging modality, and the hip was the most imaged region of interest (ROI). Regardless of pairing, imaging modality and ROI, there was often a trend toward significant familial resemblance and heritability (h2 and h2L). Conclusion: This scoping review highlights the trends in bone health linked to familial resemblance, as well as the importance of menopause and late adolescence. This review compiles the commonalities and challenges within these studies to inform future research.
Efficient Compressive Strength Prediction of Alkali-Activated Waste Materials Using Machine Learning
Authors: Hsu Chien-Hua , Chan Hao-Yu , Chang Ming-Hui , Liu Chiung-Fang , Liu Tzu-Yu , Chiu Kuo-Chuang
Published: June 2024

This study explores the integration of machine learning (ML) techniques to predict and optimize the compressive strength of alkali-activated materials (AAMs) sourced from four industrial waste streams: blast furnace slag, fly ash, reducing slag, and waste glass. Aimed at mitigating the labor-intensive trial-and-error method in AAM formulation, ML models can predict the compressive strength and then streamline the mixture compositions. By leveraging a dataset of only 42 samples, the Random Forest (RF) model underwent fivefold cross-validation to ensure reliability. Despite challenges posed by the limited datasets, meticulous data processing steps facilitated the identification of pivotal features that influence compressive strength. Substantial enhancement in predicting compressive strength was achieved with the RF model, improving the model accuracy from 0.05 to 0.62. Experimental validation further confirmed the ML model’s efficacy, as the formulations ultimately achieved the desired strength threshold, with a significant 59.65% improvement over the initial experiments. Additionally, the fact that the recommended formulations using ML methods only required about 5 min underscores the transformative potential of ML in reshaping AAM design paradigms and expediting the development process.
Experimental Analysis of the Low-Velocity Impact and CAI Properties of 3D Four-Directional Braided Composites after Hygrothermal Aging
Authors: Zhang Yuxuan , Li Hanhua , Yan Shi , Wang Xin , Guan Yue , Du Changmei , Jiang Lili , Zhai Junjun
Published: June 2024

Three-dimensional braided composites (3D-BCs) have better specific strength and stiffness than two-dimensional planar composites (2D-PCs), so they are widely used in modern industrial fields. In this paper, two kinds of 3D four-directional braided composites (3D4d-BCs) with different braided angles (15°, denoted as H15, and 30°, denoted as H30) were subjected to hydrothermal aging treatments, low-velocity impact (LVI) tests, and compression after impact (CAI) tests under different conditions. This study systematically studied the hygroscopic behavior and the effect of hygrothermal aging on the mechanical properties of 3D4d-BC. The results show that higher temperatures and smaller weaving angles can significantly improve the moisture absorption equilibrium content. When the moisture absorption content is balanced, the energy absorption effect of 3D4d-BC is better, but the integrity and residual compression rate will be reduced. Due to the intervention of oxygen molecules, the interface properties between the matrix and the composite material will be reduced, so the compressive strength will be further reduced. In the LVI test, the peak impact load of H15 is low. In CAI tests, the failure of H15 mainly occurs on the side, and the failure form is buckling failure. The main failure direction of H30 is 45° shear failure.
Research on Hot Deformation Rheological Stress of Al-Mg-Si-Mn-Sc Aluminium Alloy
Authors: Sun Wei , Zhang Yu , Yu Fang , Yang Lingfei , Song Dongfu , He Guozhong , Tong Weiping , Wang Xiangjie
Published: June 2024

The hot compression simulation testing machine was utilized to conduct compression experiments on an Al-Mg-Si-Mn alloy containing the rare earth element Sc at a deformation temperature ranging from 450 to 550 °C and a strain rate of 0.01 to 10 s−1. The study focused on the hot deformation behavior of the aluminum alloy, resulting in the determination of the optimal range of deformation process parameters for the alloy. The relationship between material flow stress, deformation temperature, and strain rate was described using the Arrhenius relationship containing thermal activation energy based on the stress-strain curve of hot compression deformation of aluminum alloy. This led to calculations for structural factor A, stress index n, and stress level parameters as well as thermal deformation activation energy to establish a constitutive Formula for hot deformation rheological stress of aluminum alloy and calculate the power dissipation factor η. Through this process, an optimized range for the optimal deformation process parameter for aluminum alloy was determined (deformation temperature: 490~510 °C; strain rate: 0.05 s−1) and verified in combination with mechanical properties and microstructure through hot extrusion deformation trial production.
Experiment and Simulation of Liquid Film Flow Driving Abrasive Particle Dispersion on the Surface of a Rotating Disk
Authors: Fu Qiong , Shi Weibin , Duan Nian , Huang Hui , Zhang Yong
Published: June 2024

Controlling the distribution of the abrasive grains on the surface of the grinding tools in an appropriate way is important for improving the quality of grinding processing and meeting the workpiece precision requirements. In the present study, a novel method for the orderly arrangement of abrasive particles is proposed by using the liquid film flow on the surface of the rotating disk as the driving and controlling means for the uniform dispersion and position arrangement of abrasive particles. Computational fluid dynamics (CFD) simulations have been performed to clearly illustrate the trajectories of abrasive particles under the strong influence of liquid film flow on the rotating disk and reveal the effects of fluid flow, disk rotational motion, and the mixture viscosity on the particle distribution. A new abrasive grain arrangement device is designed and fabricated using this novel method. The operating parameters such as liquid volume flow rate, disk rotational speed, and liquid viscosity are adjusted to control the placement of abrasive grains on the surface of the grinding tool. An image processing tool is used to examine and analyze the arrangement results. The experimental results indicated that the application of the liquid film flow on a rotating disk to the abrasive grain arrangement can improve the arrangement of abrasive grains and get rid of the dependence on the template.
From Innovation to Standardization—A Century of Rolling Bearing Life Formula
Authors: Lazović Tatjana , Marinković Aleksandar , Atanasovska Ivana , Sedak Miloš , Stojanović Blaža
Published: June 2024

This review paper is an homage to Arvid Palmgren’s pioneering paper on rolling bearing service life to highlight its relevance a century later. It follows the evolution of bearing service life theory from Palmgren’s fundamental research to the contemporary international standard ISO 281. Palmgren’s theory, based on the previously published papers of Stribeck and Hertz, laid the basis for the later development of bearing service life assessment methodology. Based on the Weibull theory of probability of damage, Lundberg and Palmgren introduced stochastic elements to explain the effect of reliability on bearing service life prediction. Harris and Ioannides, who made a significant contribution to the revision of the international standard on rolling bearing load rating and rating life are mentioned as well. Zaretsky’s critical analysis also was not neglected in this review, due to a different approach respecting the original influence of material properties and bearing performances. Despite standardization, ongoing research by leading advanced bearing industries and academic institutions continues to refine methodologies for service life assessment. Through a comprehensive review and analysis, this paper offers insight into the current state of bearing service life theory, highlighting the collaborative efforts bringing progress in this field.
Research on Auxetic Lattice Structure for Impact Absorption in Machines and Mechanisms
Authors: Széles Levente , Horváth Richárd , Cveticanin Livija
Published: June 2024

In this paper, a new type of filled doubly re-entrant auxetic lattice structure for application in damping and energy absorption devices is considered. The structure is modeled to give protection for machines and mechanisms of intensive impact. The suggested structure is the modified version of the auxetic one with silicone fillings. The unit of the structure is assumed as a re-entrant hexagon with four quadrangular absorbers. For the assumed model of unit, the deformation properties and the Poisson’s ratio were computed. The obtained results were experimentally tested. Specimens of filled and unfilled structures were investigated under quasi-static compression. The measured results show that the energy dissipation is more than two times higher for filled structure than for unfilled ones. In the filled structure, the absorber’s rigidity has the crucial role. If the rigidity is small, the absorber, inside the unit, continues to deform from rectangle into rhomboid. Otherwise, if the rigidity is high, units with absorbers form a beam-like structure that buckles and shows high energy absorption effect. The experimentally obtained results are in good agreement with the theoretical ones.
Mathematics of a Process Algebra Inspired by Whitehead’s Process and Reality: A Review
Authors: Sulis William
Published: June 2024

Process algebras have been developed within computer science and engineering to address complicated computational and manufacturing problems. The process algebra described herein was inspired by the Process Theory of Whitehead and the theory of combinatorial games, and it was developed to explicitly address issues particular to organisms, which exhibit generativity, becoming, emergence, transience, openness, contextuality, locality, and non-Kolmogorov probability as fundamental characteristics. These features are expressed by neurobehavioural regulatory systems, collective intelligence systems (social insect colonies), and quantum systems as well. The process algebra has been utilized to provide an ontological model of non-relativistic quantum mechanics with locally causal information flow. This paper provides a pedagical review of the mathematics of the process algebra.
A Security-Enhanced Federated Learning Scheme Based on Homomorphic Encryption and Secret Sharing
Authors: Shen Cong , Zhang Wei , Zhou Tanping , Zhang Lingling
Published: June 2024

Although federated learning is gaining prevalence in smart sensor networks, substantial risks to data privacy and security persist. An improper application of federated learning techniques can lead to critical privacy breaches. Practical and effective privacy-enhanced federated learning (PEPFL) is a widely used federated learning framework characterized by low communication overhead and efficient encryption and decryption processes. Initially, our analysis scrutinized security vulnerabilities within the PEPFL framework and identified an effective attack strategy. This strategy enables the server to derive private keys from content uploaded by participants, achieving a 100% success rate in extracting participants’ private information. Moreover, when the number of participants does not exceed 300, the attack time does not surpass 3.72 s. Secondly, this paper proposes a federated learning model that integrates homomorphic encryption and secret sharing. By using secret sharing among participants instead of secure multi-party computation, the amount of effective information available to servers is reduced, thereby effectively preventing servers from inferring participants’ private gradients. Finally, the scheme was validated through experiments, and it was found to significantly reduce the inherent collusion risks unique to the federated learning scenario. Moreover, even if some participants are unavailable, the reconstructable nature of secret sharing ensures that the decryption process can continue uninterrupted, allowing the remaining users to proceed with further training. Importantly, our proposed scheme exerts a negligible impact on the accuracy of model training.
Improving Water and Energy Resource Management: A Comparative Study of Solution Representations for the Pump Scheduling Optimization Problem
Authors: Silva-Rubio Sergio A. , Salgueiro Yamisleydi , Mora-Meliá Daniel , Gutiérrez-Bahamondes Jimmy H.
Published: June 2024

Water distribution networks (WDNs) are vital for communities, facing threats like climate change and aging infrastructure. Optimizing WDNs for energy and water savings is challenging due to their complexity. In particular, pump scheduling stands out as a fundamental tool for optimizing both resources. Metaheuristics such as evolutionary algorithms (EAs) offer promising solutions, yet encounter limitations in robustness, parameterization, and applicability to real-sized networks. The encoding of decision variables significantly influences algorithm efficiency, an aspect frequently overlooked in the literature. This study addresses this gap by comparing solution representations for a multiobjective pump scheduling problem. By assessing metrics such as execution time, convergence, and diversity, it identifies effective representations. Embracing a multiobjective approach enhances comprehension and solution robustness. Through empirical validation across case studies, this research contributes insights for the more efficient optimization of WDNs, tackling critical challenges in water and energy management. The results demonstrate significant variations in the performance of different solution representations used in the literature. In conclusion, this study not only provides perspectives on effective pump scheduling strategies but also aims to guide future researchers in selecting the most suitable representation for optimization problems.
Effect of Education on Adherence to Recommended Prenatal Practices among Indigenous Ngäbe–Buglé Communities of Panama
Authors: Johnson Sabrina M. , Kelly Erin N. , LaBrot Benjamin , Ryczak Kristen
Published: June 2024

Background and Objectives: The primary objective of this study was to assess the adherence of Ngäbe–Buglé women to WHO-recommended prenatal practices. The secondary objective was to compare adherence levels between women who received prenatal education from official medical providers and those educated through traditional or community-based sources within Ngäbe–Buglé communities. Materials and Methods: An eight-question survey was verbally administered to 137 Ngäbe–Buglé women at clinics set up by the non-profit NGO Floating Doctors in eight communities. A two-sided Fisher’s Exact test with a p = 0.05 was used to compare the results of mothers who received prenatal education from evidence-based sources to other groups. Results: Out of the 137 surveyed women, 65 reported taking prenatal vitamins, 21 had prenatal check-ups, 136 avoided alcohol, 31 increased caloric intake, and 102 maintained their activity levels. Significant differences were observed in prenatal vitamin adherence between those educated by official sources versus unofficial sources (p = 0.0029) and official sources compared to those with no prenatal education (p < 0.0001). The difference was also significant for education from an unofficial source versus no education (p = 0.0056). However, no significant differences were found in other prenatal practices based on education sources. Conclusions: Our findings highlight deficiencies in both prenatal education and adherence to recommended practices among Ngäbe–Buglé women. Prenatal education significantly improved adherence to taking prenatal vitamins, suggesting its effectiveness as an intervention. Future interventions should prioritize culturally competent prenatal education and address barriers to accessing prenatal healthcare in Ngäbe–Buglé communities.
A Comparative Analysis of Bacterial and Fungal Communities in Coastal and Inland Pecan Plantations
Authors: Zhang Shijie , Chen Ting , Chen Yu , Li Shucheng , Wang Wu , Zhao Yuqiang , Zhu Cancan
Published: June 2024

Pecan forests (Carya illinoinensis) are significant contributors to both food and oil production, and thrive in diverse soil environments, including coastal regions. However, the interplay between soil microbes and pecan forest health in coastal environments remains understudied. Therefore, we investigated soil bacterial and fungal diversity in coastal (Dafeng, DF) and inland (Guomei, GM) pecan plantations using high-throughput sequencing. The results revealed a higher microbial diversity in the DF plantation than in the GM plantation, significantly influenced by pH and edaphic factors. The dominant bacterial phyla were Proteobacteria, Acidobacteriota and Bacteroidota in the DF plantation, and Acidobacteriota, Proteobacteria, and Verrucomicrobiota in the GM plantation. Bacillus, Nitrospira and UTCFX1 were significantly more abundant bacterial genera in DF soil, whereas Candidatus Udaeobacter, HSB_OF53-F07 and ADurbBin063-1 were more prevalent in GM soil. Basidiomycota dominated fungal sequences in the GM plantation, with a higher relative abundance of Ascomycota in the DF plantation. Significant differences in fungal genus composition were observed between plantations, with Scleroderma, Hebeloma, and Naucoria being more abundant in DF soil, and Clavulina, Russula, and Inocybe in GM soil. A functional analysis revealed greater carbohydrate metabolism potential in GM plantation bacteria and a higher ectomycorrhizal fungi abundance in DF soil. Significantly positive correlations were detected between certain bacterial and fungal genera and pH and total soluble salt content, suggesting their role in pecan adaptation to coastal environments and saline–alkali stress mitigation. These findings enhance our understanding of soil microbiomes in coastal pecan plantations, and are anticipated to foster ecologically sustainable agroforestry practices and contribute to coastal marshland ecosystem management.
Rational Design of Flexible, Self-Supporting, and Binder-Free Prussian White/KetjenBlack/MXene Composite Electrode for Sodium-Ion Batteries with Boosted Electrochemical Performance
Authors: Dai Xiaowen , Chun Jingyun , Wang Xiaolong , Xv Tianao , Wang Zhengran , Wei Chuanliang , Feng Jinkui
Published: June 2024

Due to their cost-effectiveness, abundant resources, and suitable working potential, sodium-ion batteries are anticipated to establish themselves as a leading technology in the realm of grid energy storage. However, sodium-ion batteries still encounter challenges, including issues related to low energy density and constrained cycling performance. In this study, a self-supported electrode composed of Prussian white/KetjenBlack/MXene (TK−PW) is proposed. In the TK−PW electrode, the MXene layer is coated with Prussian white nanoparticles and KetjenBlack with high conductivity, which is conducive to rapid Na+ dynamics and effectively alleviates the expansion of the electrode. Notably, the electrode preparation method is uncomplicated and economically efficient, enabling large-scale production. Electrochemical testing demonstrates that the TK−PW electrode retains 74.9% of capacity after 200 cycles, with a discharge capacity of 69.7 mAh·g−1 at 1000 mA·g−1. Furthermore, a full cell is constructed, employing a hard carbon anode and TK−PW cathode to validate the practical application potential of the TK−PW electrode.
New 3-(Dibenzyloxyphosphoryl)isoxazolidine Conjugates of N1-Benzylated Quinazoline-2,4-diones as Potential Cytotoxic Agents against Cancer Cell Lines
Authors: Łysakowska Magdalena , Głowacka Iwona E. , Honkisz-Orzechowska Ewelina , Handzlik Jadwiga , Piotrowska Dorota G.
Published: June 2024

In this study, a new series of cis and trans 5-substituted-3-(dibenzyloxyphosphoryl)isoxazolidines 16a–g were synthesized by the 1,3-dipolar cycloaddition reaction of N-benzyl-C-(dibenzyloxyphosphoryl)nitrone and selected N1-allyl-N3-benzylquinazoline-2,4-diones. All the obtained trans-isoxazolidines 16a–g and the samples enriched in respective cis-isomers were evaluated for anticancer activity against three tumor cell lines. All the tested compounds exhibited high activity against the prostate cancer cell line (PC-3). Isoxazolidines trans-16a and trans-16b and diastereoisomeric mixtures of isoxazolidines enriched in cis-isomer using HPLC, namely cis-16a/trans-16a (97:3) and cis-16b/trans-16b (90:10), showed the highest antiproliferative properties towards the PC-3 cell line (IC50 = 9.84 ± 3.69–12.67 ± 3.45 μM). For the most active compounds, induction apoptosis tests and an evaluation of toxicity were conducted. Isoxazolidine trans-16b showed the highest induction of apoptosis. Moreover, the most active compounds turned out safe in vitro as none affected the cell viability in the HEK293, HepG2, and HSF cellular models at all the tested concentrations. The results indicated isoxazolidine trans-16b as a promising new lead structure in the search for effective anticancer drugs.
Binding Energies and Optical Properties of Power-Exponential and Modified Gaussian Quantum Dots
Authors: Alauwaji Ruba Mohammad , Dakhlaoui Hassen , Algraphy Eman , Ungan Fatih , Wong Bryan M.
Published: June 2024

We examine the optical and electronic properties of a GaAs spherical quantum dot with a hydrogenic impurity in its center. We study two different confining potentials: (1) a modified Gaussian potential and (2) a power-exponential potential. Using the finite difference method, we solve the radial Schrodinger equation for the 1s and 1p energy levels and their probability densities and subsequently compute the optical absorption coefficient (OAC) for each confining potential using Fermi’s golden rule. We discuss the role of different physical quantities influencing the behavior of the OAC, such as the structural parameters of each potential, the dipole matrix elements, and their energy separation. Our results show that modification of the structural physical parameters of each potential can enable new optoelectronic devices that can leverage inter-sub-band optical transitions.
Stable Radical Isoporphyrin Copolymer Prepared with Di(phenylphosphane)
Authors: Liang Yiming , Bonnefont Antoine , Badets Vasilica , Boudon Corinne , Goldmann Michel , Diot Guillaume , Choua Sylvie , Le Breton Nolwenn , Ruhlmann Laurent
Published: June 2024

Two diphosphanes with variable-length ligands tested as nucleophiles to prepare isoporphyrin copolymers in the presence of ditolylporphyrin of zinc (ZnT2P) prevented the oxidation of the diphosphine ligand. This paper demonstrates the power of this approach and describes the photoelectrocatalytic properties. The obtained copolymers were characterized by UV–vis spectroscopy, X-ray photoelectron spectroscopy, atomic force micrograph (AFM), EQCM (Electrochemical Quartz Cristal Microbalance) and electrochemistry. Their impedance properties (EIS) were studied and their photovoltaic performances were also investigated by photocurrent transient measurements under visible light irradiation.
A Mitochondria-Targeting Fluorescent Probe for the Dual Sensing of Hypochlorite and Viscosity without Signal Crosstalk in Living Cells and Zebrafish
Authors: Gao Chao , Chen Dan-Dan , Zhang Lin , Ma Ming-Lan , Liu Hu-Wei , Cui Hai-Rong
Published: June 2024

Hypochlorite (ClO−) and viscosity both affect the physiological state of mitochondria, and their abnormal levels are closely related to many common diseases. Therefore, it is vitally important to develop mitochondria-targeting fluorescent probes for the dual sensing of ClO− and viscosity. Herein, we have explored a new fluorescent probe, XTAP–Bn, which responds sensitively to ClO− and viscosity with off–on fluorescence changes at 558 and 765 nm, respectively. Because the emission wavelength gap is more than 200 nm, XTAP–Bn can effectively eliminate the signal crosstalk during the simultaneous detection of ClO− and viscosity. In addition, XTAP–Bn has several advantages, including high selectivity, rapid response, good water solubility, low cytotoxicity, and excellent mitochondrial-targeting ability. More importantly, probe XTAP–Bn is successfully employed to monitor the dynamic change in ClO− and viscosity levels in the mitochondria of living cells and zebrafish. This study not only provides a reliable tool for identifying mitochondrial dysfunction but also offers a potential approach for the early diagnosis of mitochondrial-related diseases.
Nano-Based Approaches in Surface Modifications of Dental Implants: A Literature Review
Authors: Marasli Chrysa , Katifelis Hector , Gazouli Maria , Lagopati Nefeli
Published: June 2024

Rehabilitation of fully or partially edentulous patients with dental implants represents one of the most frequently used surgical procedures. The work of Branemark, who observed that a piece of titanium embedded in rabbit bone became firmly attached and difficult to remove, introduced the concept of osseointegration and revolutionized modern dentistry. Since then, an ever-growing need for improved implant materials towards enhanced material–tissue integration has emerged. There is a strong belief that nanoscale materials will produce a superior generation of implants with high efficiency, low cost, and high volume. The aim of this review is to explore the contribution of nanomaterials in implantology. A variety of nanomaterials have been proposed as potential candidates for implant surface customization. They can have inherent antibacterial properties, provide enhanced conditions for osseointegration, or act as reservoirs for biomolecules and drugs. Titania nanotubes alone or in combination with biological agents or drugs are used for enhanced tissue integration in dental implants. Regarding immunomodulation and in order to avoid implant rejection, titania nanotubes, graphene, and biopolymers have successfully been utilized, sometimes loaded with anti-inflammatory agents and extracellular vesicles. Peri-implantitis prevention can be achieved through the inherent antibacterial properties of metal nanoparticles and chitosan or hybrid coatings bearing antibiotic substances. For improved corrosion resistance various materials have been explored. However, even though these modifications have shown promising results, future research is necessary to assess their clinical behavior in humans and proceed to widespread commercialization.
Isovaleryl Sucrose Esters from Atractylodes japonica and Their Cytotoxic Activity
Authors: Wang Yimeng , Wang Zhibin , Sun Yanping , Zhu Mingtao , Jiang Yong , Bai Haodong , Yang Bingyou , Kuang Haixue
Published: June 2024

Cancer represents one of the most significant health challenges currently facing humanity, and plant-derived antitumour drugs represent a prominent class of anticancer medications in clinical practice. Isovaleryl sucrose esters, which are natural constituents, have been identified as having potential antitumour effects. However, the mechanism of action remains unclear. In this study, 12 isovaleryl sucrose ester components, including five new (1–5) and seven known compounds (6–12), were isolated from the roots of Atractylodes japonica. The structures of the compounds were elucidated using 1D and 2D-NMR spectroscopy, complemented by HR-ESI-MS mass spectrometry. The cytotoxic activities of all the compounds against human colon cancer cells (HCT-116) and human lung adenocarcinoma cells (A549) were also evaluated using the CCK8 assay. The results demonstrated that compounds 2, 4, and 6 were moderately inhibitory to HCT-116 cells, with IC50 values of 7.49 ± 0.48, 9.03 ± 0.21, and 13.49 ± 1.45 μM, respectively. Compounds 1 and 6 were moderately inhibitory to A549, with IC50 values of 8.36 ± 0.77 and 7.10 ± 0.52 μM, respectively. Molecular docking revealed that compounds 1–9 exhibited a stronger affinity for FGFR3 and BRAF, with binding energies below −7 kcal/mol. Compound 2 exhibited the lowest binding energy of −10.63 kcal/mol to FGFR3. We screened the compounds with lower binding energies, and the protein-ligand complexes already obtained after molecular docking were subjected to exhaustive molecular dynamics simulation experiments, which simulated the dynamic behaviour of the molecules in close proximity to the actual biological environment, thus providing a deeper understanding of their functions and interaction mechanisms. The present study provides a reference for the development and use of iso-valeryl sucrose esters in the antitumour field.
Enzymatic Fructosylation of Phenolic Compounds: A New Alternative for the Development of Antidiabetic Drugs
Authors: Damian-Medina Karla , Herrera-González Azucena , Figueroa-Yáñez Luis J. , Arrizon Javier
Published: June 2024

Enzymatic fructosylation has emerged as a strategy to enhance the hydrophilicity of polyphenols by introducing sugar moieties, leading to the development of phenolic glycosides, which exhibit improved solubility, stability, and biological activities compared to their non-glycosylated forms. This study provides a detailed analysis of the interactions between five phenolic fructosides (4MFPh, MFF, DFPh, MFPh, and MFPu) and twelve proteins (11β-HS1, CRP, DPPIV, IRS, PPAR-γ, GK, AMPK, IR, GFAT, IL-1ß, IL-6, and TNF-α) associated with the pathogenesis of T2DM. The strongest interactions were observed for phlorizin fructosides (DFPh) with IR (−16.8 kcal/mol) and GFAT (−16.9 kcal/mol). MFPh with 11β-HS1 (−13.99 kcal/mol) and GFAT (−12.55 kcal/mol). 4MFPh with GFAT (−11.79 kcal/mol) and IR (−12.11 kcal/mol). MFF with AMPK (−9.10 kcal/mol) and PPAR- γ (−9.71 kcal/mol), followed by puerarin and ferulic acid monofructosides. The fructoside group showed lower free energy binding values than the controls, metformin and sitagliptin. Hydrogen bonding (HB) was identified as the primary interaction mechanism, with specific polar amino acids such as serin, glutamine, glutamic acid, threonine, aspartic acid, and lysine identified as key contributors. ADMET results indicated favorable absorption and distribution characteristics of the fructosides. These findings provide valuable information for further exploration of phenolic fructosides as potential therapeutic agents for T2DM.
Cytostatic Bacterial Metabolites Interfere with 5-Fluorouracil, Doxorubicin and Paclitaxel Efficiency in 4T1 Breast Cancer Cells
Authors: Schwarcz Szandra , Nyerges Petra , Bíró Tímea Ingrid , Janka Eszter , Bai Péter , Mikó Edit
Published: June 2024

The microbiome is capable of modulating the bioavailability of chemotherapy drugs, mainly due to metabolizing these agents. Multiple cytostatic bacterial metabolites were recently identified that have cytostatic effects on cancer cells. In this study, we addressed the question of whether a set of cytostatic bacterial metabolites (cadaverine, indolepropionic acid and indoxylsulfate) can interfere with the cytostatic effects of the chemotherapy agents used in the management of breast cancer (doxorubicin, gemcitabine, irinotecan, methotrexate, rucaparib, 5-fluorouracil and paclitaxel). The chemotherapy drugs were applied in a wide concentration range to which a bacterial metabolite was added in a concentration within its serum reference range, and the effects on cell proliferation were assessed. There was no interference between gemcitabine, irinotecan, methotrexate or rucaparib and the bacterial metabolites. Nevertheless, cadaverine and indolepropionic acid modulated the Hill coefficient of the inhibitory curve of doxorubicin and 5-fluorouracil. Changes to the Hill coefficient implicate alterations to the kinetics of the binding of the chemotherapy agents to their targets. These effects have an unpredictable significance from the clinical or pharmacological perspective. Importantly, indolepropionic acid decreased the IC50 value of paclitaxel, which is a potentially advantageous combination.
The Intersection of Food Security and Mental Health in the Pursuit of Sustainable Development Goals
Authors: Onyeaka Helen , Ejiohuo Ovinuchi , Taiwo Oluseyi Rotimi , Nnaji Nnabueze Darlington , Odeyemi Omowale A. , Duan Keru , Nwaiwu Ogueri , Odeyemi Olumide
Published: June 2024

Food insecurity, a multifaceted global challenge, intertwines with mental health concerns, necessitating nuanced strategies for sustainable solutions. The intricate web of challenges posed by these intersections has made it imperative to delineate a strategic way forward, incorporating solutions and robust policy recommendations. This study aims to comprehensively examine the intricate relationship between food security and its intersection with mental health on a global scale, offering insights into case studies, responses, and innovative approaches to inform effective strategies for addressing these pressing challenges. This study involved an analysis of a literature search, mainly between 2013 and 2023, with an updated addition of relevant 2024 studies. Examining responses across regions unveils varied interventions, from targeted social safety net programs in West Africa to technology-driven solutions in Asia. Success stories, such as Ghana’s sustainable agricultural practices and Canada’s income transfer programs, underscore the efficacy of multifaceted approaches. Innovative initiatives like community food programs offer promising alternatives to traditional food banks. Furthermore, international cooperation and policy innovations, exemplified by the European Union’s “Farm to Fork Strategy”, demonstrate the potential for collective action in addressing food insecurity. By prioritizing integrated strategies, global collaboration, and evidence-based policymaking, we lay the groundwork for sustainable development where communities thrive nutritionally and mentally. We emphasize continuous research and evaluation and incorporating mental health support into community programs to pave the way for a future where communities are not only food-secure but also mentally resilient.
Health-Related Quality of Life during 26-Week Intervention with the New Nordic Renal Diet
Authors: Hansen Nikita Misella , Kamper Anne-Lise , Rix Marianne , Feldt-Rasmussen Bo , Berg Peder , Astrup Arne , Salomo Louise
Published: June 2024

The New Nordic Renal Diet (NNRD) is a whole-food approach, tailored to meet recommended guidelines in patients with moderate chronic kidney disease (stage 3b–4). The NNRD improved various metabolic and physiological endpoints during a 26-week randomized controlled study. Here, we examined the effect of dietary intervention on health-related quality of life (HRQoL). Sixty participants were recruited (NNRD group n = 30, control group n = 30) and 58 completed the study. During the intervention, the NNRD group received food boxes, and recipes once a week. The control group continued their habitual diet. HRQoL was examined at baseline and at the end of the intervention using the validated EuroQol-5D-5L, including a 5-point scale Likert questionnaire at the end of the intervention. Assessed by the EuroQol-5D-5L questionnaire, the NNRD group experienced a reduction in pain/discomfort during the intervention by 26% [−0.44 points (95% CI; −0.73, −0.16)], compared with no change in the control group [0.25 points (95% CI; −0.02, 0.53)] and a between-group difference of −0.70 points (95% CI; −1.03, −0.37, p < 0.001). A larger decrease of body fat mass was associated with a larger decrease in pain/discomfort (p = 0.014). In addition, the NNRD group reported an overall improvement in conducting usual daily activities by 23% [−0.30-point (95% CI; −0.50, −0.11)], while no change was seen in the control group [−0.02 points (95% CI; −0.21, 0.17)], with a between-group difference −0.28 points (95% CI; −0.51, −0.06, p = 0.014). A larger decrease in 24 h urine phosphorus excretion, used as a marker of compliance, was associated with a larger improvement in conducting usual daily activities (p = 0.036). The NNRD group had a clinically relevant improvement in various HRQoL outcomes.
Neurodevelopmental Outcomes of Preschoolers with Antenatal Zika Virus Exposure Born in the United States
Authors: Mulkey Sarah B. , Corn Elizabeth , Williams Meagan E. , Ansusinha Emily , Podolsky Robert H. , Arroyave-Wessel Margarita , Vezina Gilbert , Peyton Colleen , Msall Michael E. , DeBiasi Roberta L.
Published: June 2024

Neurodevelopmental outcomes for preschool-age children in the United States with in utero Zika virus (ZIKV) exposure have not yet been reported. We performed a case-control study to assess whether children exposed in utero to ZIKV have abnormal neurodevelopment at age 4–5 years compared to unexposed controls. Thirteen ZIKV-exposed cases that did not have microcephaly or other specific features of congenital Zika syndrome and 12 controls were evaluated between ages 4–5 years. Child neurodevelopment was assessed using the Pediatric Evaluation of Disability Inventory, Behavior Rating Inventory of Executive Function, Peabody Picture Vocabulary Test, Bracken School Readiness Assessment (BSRA), and Movement Assessment Battery for Children (MABC). Caregivers answered questions on the child’s medical history and family demographics. Cases and controls were evaluated at mean (SD) ages 4.9 (0.3) and 4.8 (0.4) years, respectively. Caregivers reported more behavior and mood problems in cases than controls. MABC scores showed more gross and fine motor coordination difficulties among cases than controls. Controls trended towards higher performance on concepts underlying school readiness on BSRA. Three cases had a diagnosis of autism spectrum disorder or global developmental delay. Continued follow-up through school age for children with prenatal ZIKV exposure is needed to understand the impact of in utero ZIKV exposure on motor coordination, cognition, executive function, and academic achievement.
An In Silico Study Based on QSAR and Molecular Docking and Molecular Dynamics Simulation for the Discovery of Novel Potent Inhibitor against AChE
Authors: Khedraoui Meriem , Abchir Oussama , Nour Hassan , Yamari Imane , Errougui Abdelkbir , Samadi Abdelouahid , Chtita Samir
Published: June 2024

Acetylcholinesterase (AChE) is one of the main drug targets for treating Alzheimer’s disease. This current study relies on multiple molecular modeling approaches to develop new potent inhibitors of AChE. We explored a 2D QSAR study using the statistical method of multiple linear regression based on a set of substituted 5-phenyl-1,3,4-oxadiazole and N-benzylpiperidine analogs, which were recently synthesized and proved their inhibitory activities against acetylcholinesterase (AChE). The molecular descriptors, polar surface area, dipole moment, and molecular weight are the key structural properties governing AChE inhibition activity. The MLR model was selected based on its statistical parameters: R2 = 0.701, R2test = 0.76, Q2CV = 0.638, and RMSE = 0.336, demonstrating its predictive reliability. Randomization tests, VIF tests, and applicability domain tests were adopted to verify the model’s robustness. As a result, 11 new molecules were designed with higher anti-Alzheimer’s activities than the model molecule. We demonstrated their improved pharmacokinetic properties through an in silico ADMET study. A molecular docking study was conducted to explore their AChE inhibition mechanisms and binding affinities in the active site. The binding scores of compounds M1, M2, and M6 were (−12.6 kcal/mol), (−13 kcal/mol), and (−12.4 kcal/mol), respectively, which are higher than the standard inhibitor Donepezil with a binding score of (−10.8 kcal/mol). Molecular dynamics simulations over 100 ns were used to validate the molecular docking results, indicating that compounds M1 and M2 remain stable in the active site, confirming their potential as promising anti-AChE inhibitors.
Second Harmonic Generation of Twisted Vector Vortex Beams Using aβ-BaB2O4 Crystal
Authors: Liu Chang , Guan Ying , Wu Yan , Hu Xiaobo , Chew Khian-Hooi , Chen Rui-Pin
Published: June 2024

In this study, we demonstrate both theoretically and experimentally second harmonic generation (SHG) of a twisted vector vortex optical field (TVVOF) using a nonlinear type-II phase-matched β-BaB2O4 (BBO) crystal. Our study introduces a novel method to manipulate SHG by independently modulating the two orthogonal polarization components of a TVVOF. This flexibility in controlling SHG can be achieved through accurate experimental adjustments of the polarization components. Furthermore, we reveal that the SHG can be dynamically tuned by varying the angle between the polarization direction of the optical field and the principal axis of the BBO crystal via rotation. These findings provide a new approach for the flexible manipulation of SHG in structured vector optical fields, which have potential applications in optical communication, quantum optics, and photonic device engineering.
Efficient Optical Coupling between Dielectric Strip Waveguides and a Plasmonic Trench Waveguide
Authors: Wu Jia-Ren , Chandel Anjali , Chuang Chiashain , Chang Sheng Hsiung
Published: June 2024

Buttcoupling is the most efficient way to excite surface plasmon polariton (SPP) waves at dielectric/metal interfaces in order to realize applications in broadband and ultra-compact integrated circuits (IOCs). We propose a reasonable waveguide structure to efficiently excite and collect the SPP waves supported in a plasmonic trench waveguide in the long-haul telecommunication wavelength range. Our simulation results show that the coupling efficiency between the dielectric strip waveguides and a plasmonic trench waveguide can be optimized, which is dominated by the zigzag propagation path length in the dielectric strip loaded on the metal substrate. It is noted that nearly a 100% coupling efficiency can be achieved when the distance between the excitation source and the plasmonic waveguide is about 6.76 μm.
Hydroalcoholic Extracts from Pleurotus ostreatus Spent Substrate with Nematocidal Activity against Nacobbus aberrans Phytonematode and the Non-Target Species Panagrellus redivivus
Authors: Cruz-Arévalo Julio , Hernández-Velázquez Víctor M. , Cardoso-Taketa Alexandre Toshirrico , González-Cortazar Manases , Sánchez-Vázquez José E. , Peña-Chora Guadalupe , Villar-Luna Edgar , Aguilar-Marcelino Liliana
Published: June 2024

Pleurotus ostreatus, an edible mushroom widely consumed worldwide, generates a by-product known as spent mushroom substrate (SMS). This material has demonstrated biological activity against agricultural crop pathogens. In this study, we evaluated the nematocidal effectiveness of hydroalcoholic extracts (T5, T2, AT5, and AT2) derived from SMS of P. ostreatus against (J2) of the phytonematode Nacobbus aberrans and assessed their potential toxicity towards the non-target nematode Panagrellus redivivus. Among these extracts, AT5 exhibited the highest efficacy against N. aberrans and was the least toxic against P. redivivus. Liquid–liquid partitioning yielded the AQU fraction, which showed significant nematocidal activity against J2 (75.69% ± 8.99 mortality), comparable to chitosan. The GC-MS analysis revealed the presence of several compounds, including palmitic acid, linoleic acid, and 2,4-Di-tert-butylphenol. These findings are consistent with studies confirming the antagonistic effectiveness of these compounds against phytonematodes. Additionally, all extracts exhibited toxicity against P. redivivus, with T2 being the most toxic. Our findings demonstrate that while the AT5 extract displays antagonistic effectiveness against both N. aberrans and P. redivivus, it was the least toxic among the extracts tested. Thus, SMS of P. ostreatus holds potential as a source of nematocidal compounds, which could offer significant benefits for agricultural pest control.