bluuebunny/crossref_metadata_2025 huggingface dataset:
Change of the role of cryptocurrencies in the global financial system due to armed aggression against Ukraine
Authors:   Ministry of Digital Transformation of Ukraine, PARKHOMENKO Yuliya
Published: December 2022

Introduction. Сryptocurrency as a component of Ukraine's digital war against Russia. Rapid collection of funds from all over the world to help Ukraine.The USA imposes sanctions on the Russian Federation. Russia is looking for methods to circumvent sanctions. The purpose of the research work is to develop proposals for greater effectiveness of international sanctions and to find new useful methods of using cryptocurrency for Ukraine, which is part of the international financial system. Problems. Sanction packages are applied to various sectors of the economy, and following the implementation results, the following packages of sanctions are produced by the countries, so, according to the results of the implementation of sanctions, certain Russian banking institutions have been circumvented by using crypto market tools and operators that interact with sanctioned Russian banks, companies, and individuals. The use of traditional financial services, financial technologies and alternative payment services is a more effective way of circumventing sanctions in terms of volumes and costs than the use of crypto-assets.Goal. Development of proposals to increase the influence on the implementation and effectiveness of international sanctions, the need to take quick and optimal actions to eliminate the activities of many Russian cryptocurrency tools for circumventing sanctions. Materials and methods. General scientific and research methods are used: analysis, theoretical generalization, synthesis, description, comparison, observation and study of foreign mass media articles. Results. Attention was focused on Ukraine's new positive use of cryptocurrency, which is part of the international financial system, unprecedented use of new digital assets to raise funds to support Ukraine during the war. Conclusions. Crypto assets have become Ukraine's deadly weapon against Russia. Taking into account the advantages of the circulation of cryptocurrencies and virtual assets in general, the private sector in Ukraine organized charitable funds for the accumulation of resources, among which crypto funds took a leading place and were able to quickly cover the needs of defenders of Ukraine, citizens who suffered from Russian military aggression, supported socially vulnerable Ukrainian citizens. At the same time, IT specialists and amateurs organized themselves into an IT army, which was able to completely or for significant periods stop the activity of the infrastructure of the aggressor states, which slowed down the flow of resources for the continuation and escalation of military operations.
Fiscal decentralization in Ukraine under martial law
Authors:   SESE “The Academy of Financial Management”, PAVLIUK Klavdiia , BARTOSH Serhii ,   SESE “The Academy of Financial Management”
Published: December 2022

The article examines the role of fiscal decentralization in Ukraine during martial law in ensuring the vital activities of the state. On the basis of foreign sources, it has been determined that decentralization is not a one-way process that has a linear character and leads to ambiguous consequences, although it generally has a positive effect on indicators of macroeconomic development. Scientists usually associate its success with the stabilization of the socio-economic situation, ensuring the high quality of local budgets and budget management, transparency of the budget process. It was found that decentralization in our country took place in the turbulent conditions of the hybrid war unleashed by Russia, as well as the global coronavirus pandemic. Despite this, positive results were achieved in strengthening the financial base of local authorities and self-government, and authoritative international organizations gave a high assessment of the decentralization process, including the fiscal one. The situation with the filling of local budgets during the war and the implementation of expenditures was analyzed, and the positive and contradictory consequences of the changes made to the relevant legislation were determined. The uncertain nature of the further development of events due to the impossibility of predicting the duration of active hostilities, the huge scale of destruction, the relocation of business, and the need for constant adjustment of the legal framework is emphasized. It was concluded that there is a need to develop a strategic document that would clearly outline all changes and innovations related to inter-budgetary relations and the relevant powers of local government and local authorities during the period of martial law. This would contribute to increasing the transparency of the budget process, would prevent the emergence of contradictions in the relations between central and local authorities and self-government.
Students Feedback to Online Learning on Practice Courses
Authors: Mujiono  , Marisa  , Prabowo Suryo
Published: December 2022

This study aims to determine students feedback to the application of online learning on practical courses in the Education Technology Study Program. This research is a descriptive research with a quantitative approach. The research instrument used in the form of questionnaires and interviews. Of the 104 respondents who filled out the questionnaire, as many as 88 respondents (86.3%) still wanted a synchronous meeting in the form of a webinar tutorial. The results of the interviews showed that the application of online learning in practical courses received a positive response from the majority of respondents, but the implementation time and learning assessment received a negative response.
Coffee producers’ perspectives of blockchain technology in the context of sustainable global value chains
Authors: Singh Christina , Wojewska Aleksandra N. , Persson U. Martin , Bager Simon L.
Published: December 2022

Transparency and equitability are key for improved sustainability outcomes in global value chains. Blockchain technology has been touted as a tool for achieving these ends. However, due to the limited empirical evidence, claims on transparency and sustainability benefits are largely theoretical. We lack an understanding of the benefits and drawbacks for upstream actors within global value chains and how this affects technology adoption. Addressing this gap, we conduct an empirical study to identify the drivers and obstacles for coffee producers in Colombia in adopting blockchain. We base our research on an event-driven and permissioned blockchain model, specifically designed for this research. Applying the Participation Capacity Framework and conducting semi-structured interviews with coffee producers and key informants, we analyze adoption attitudes towards the blockchain application. We further identify opportunities and drawbacks from the producers’ perspective. We set these findings in the context of the Global Value Chain research, considering the existing power relations in the coffee value chain. The top-down nature of blockchain projects raises distributive concerns, as resource investments, implementation burden, and risks are significantly higher upstream, whereas downstream lead firms will benefit most. We identify data squeeze as an additional channel of sustainable supplier squeeze relevant in the case of blockchain initiatives. Data squeeze implies lead firms turning the data obtained through, likely unpaid, labour of blockchain participants into a monetizable assets and marketable value through branding and advertisement. Based on the findings, we identify potential design dimensions and implementation features that can contribute to materializing producer benefits, thus mitigating the risk of a sustainability-driven supplier squeeze.
Endometrial small extracellular vesicles regulate human trophectodermal cell invasion by reprogramming the phosphoproteome landscape
Authors: Fatmous Monique , Rai Alin , Poh Qi Hui , Salamonsen Lois A. , Greening David W.
Published: December 2022

A series of cyclical events within the uterus are crucial for pregnancy establishment. These include endometrial regeneration following menses, under the influence of estrogen (proliferative phase), then endometrial differentiation driven by estrogen/progesterone (secretory phase), to provide a microenvironment enabling attachment of embryo (as a hatched blastocyst) to the endometrial epithelium. This is followed by invasion of trophectodermal cells (the outer layer of the blastocyst) into the endometrium tissue to facilitate intrauterine development. Small extracellular vesicles (sEVs) released by endometrial epithelial cells during the secretory phase have been shown to facilitate trophoblast invasion; however, the molecular mechanisms that underline this process remain poorly understood. Here, we show that density gradient purified sEVs (1.06–1.11 g/ml, Alix+ and TSG101+, ∼180 nm) from human endometrial epithelial cells (hormonally primed with estrogen and progesterone vs. estrogen alone) are readily internalized by a human trophectodermal stem cell line and promote their invasion into Matrigel matrix. Mass spectrometry-based proteome analysis revealed that sEVs reprogrammed trophectoderm cell proteome and their cell surface proteome (surfaceome) to support this invasive phenotype through upregulation of pro-invasive regulators associated with focal adhesions (NRP1, PTPRK, ROCK2, TEK), embryo implantation (FBLN1, NIBAN2, BSG), and kinase receptors (EPHB4/B2, ERBB2, STRAP). Kinase substrate prediction highlighted a central role of MAPK3 as an upstream kinase regulating target cell proteome reprogramming. Phosphoproteome analysis pinpointed upregulation of MAPK3 T204/T202 phosphosites in hTSCs following sEV delivery, and that their pharmacological inhibition significantly abrogated invasion. This study provides novel molecular insights into endometrial sEVs orchestrating trophoblast invasion, highlighting the microenvironmental regulation of hTSCs during embryo implantation.
Polydopamine-based nanospheres as nanoplatforms to kill Staphylococcus aureus and to promote wound healing by photothermal therapy
Authors: Hou Zhidian , Yang Min , Huang Ling , Xin Songlin , Yang Huiming , Hou Jiangping
Published: December 2022

Bacterial infections have always been a threat when it comes to public health accounting for increased morbidity and mortality rates around the world. For the first time, Polydopamine is often used as an ocular surface drug delivery medium to treat some ocular surface diseases based on its good tissue affinity. Mesoporous polydopamine nanospheres (MPDA NPs) under photothermal therapy (PTT) are demonstrated as efficient therapeutic nanoplatforms for Staphylococcus aureus (S. aureus) infection and wound healing. MPDA NPs were found to exhibit excellent photothermal performance, significantly causing an increase in temperature within a short period of NIR-I exposure (808 nm, 1 W cm−2, 6 min). The MPDA NPs under the NIR irradiation remarkably eliminated S. aureus in vitro. Moreover, these synergistic effects turnouts to be phenomenal in vivo, effectively killing and healing S. aureus-infected abscesses in mice. These revealed the combined effect of the intrinsic antibacterial activity of MPDA NPs enhanced upon NIR-I exposure. Hence, MPDA NPs under NIR-I could prove excellent therapeutic nanoplatforms for bacteria-related infections and other biomedical applications.
Multi-omics reveals specific host metabolism-microbiome associations in intracerebral hemorrhage
Authors: Chen Lei , Wang Sai , Zhang Yupeng , Li Ye , Zhang Xiangbin , Ma Junyi , Zou Xuelun , Yao TianXing , Li Si , Chen Junyou , Zhou Huifang , Wu Lianxu , Zhou Yanhong , Zhang Le
Published: December 2022

Intracerebral hemorrhage (ICH) is the most devastating subtype of stroke, but effective prevention and treatment strategies are lacking. Recently, gut microbiome and its metabolitesis are considered to be an influencing factor of stroke. However, little is known about the effects of the gut microbiome on ICH and host metabolic activity. Therefore, we used 16S sequencing, macrogenomics sequencing and untargeted metabolomics to explore the differences in gut microbial-metabolome interactions between patients with intracerebral hemorrhage and healthy control populations. We found a significant decrease in the phylum of Firmicutes and a significant increase of Bacteroidetes in ICH patients. At the genus level, Streptococcus, Bifidobacterium, Akkermansia, and Lactobacillus were more abundant in ICH patients. Macrogenomic analysis revealed active glycosaminoglycan degradation, heme synthesis, galactose degradation, lipopolysaccharide core region synthesis, and beta-Lactam resistance in ICH patients. Serum untargeted metabolomic analysis combined with ROC curves showed that octanoylcarnitine, decanoylcarnitine, dodecanoylcarnitine, glyceric acid, pyruvic acid, aspartic acid, methylcysteine, pyroglutamic acid, 9E-tetradecenoic acid, N-Acetylneuraminic acid, and aconitic acid were the best markers for the diagnosis of ICH. Correlation analysis showed that microbiome enriched in the gut of ICH patients were significantly correlated with serum metabolites, revealing a close correlation between the gut microbiome of ICH patients and the host metabolome, and significant differences from the healthy population. microbiota-host co-metabolites including pyruvic acid and 9E-tetradecenoic acid is associated with the the National Institutes of Health Stroke Scale (NIHSS) scores. In conclusion, microbiome-related metabolites in ICH patients was associated with the severity of ICH, the microbiota-host co-metabolites may be a potential may be potential therapeutic targets.
A tomographic microscopy-compatible Langendorff system for the dynamic structural characterization of the cardiac cycle
Authors: Dejea Hector , Schlepütz Christian M. , Méndez-Carmona Natalia , Arnold Maria , Garcia-Canadilla Patricia , Longnus Sarah L. , Stampanoni Marco , Bijnens Bart , Bonnin Anne
Published: December 2022

Introduction

Cardiac architecture has been extensively investigated ex vivo using a broad spectrum of imaging techniques. Nevertheless, the heart is a dynamic system and the structural mechanisms governing the cardiac cycle can only be unveiled when investigating it as such.

Methods

This work presents the customization of an isolated, perfused heart system compatible with synchrotron-based X-ray phase contrast imaging (X-PCI).

Results

Thanks to the capabilities of the developed setup, it was possible to visualize a beating isolated, perfused rat heart for the very first time in 4D at an unprecedented 2.75 μm pixel size (10.6 μm spatial resolution), and 1 ms temporal resolution.

Discussion

The customized setup allows high-spatial resolution studies of heart architecture along the cardiac cycle and has thus the potential to serve as a tool for the characterization of the structural dynamics of the heart, including the effects of drugs and other substances able to modify the cardiac cycle.
A nomogram prediction model for early death in patients with persistent pulmonary hypertension of the newborn
Authors: Lin Chuyang , Mi Jiao , Zhang Yinyue , Duan Sichen , Wu Jinlin , Li Yifei
Published: December 2022

Background

Persistent pulmonary hypertension of the newborn (PPHN) is a major lethal disorder in neonates that leads to an extremely high mortality rate. Thus, the early identification of adverse outcomes in PPHN is critical for clinical practice. This research attempted to develop a nomogram prediction system for assessing the mortality of newborns with PPHN.

Methods

Two hundred and three newborns with PPHN diagnosed from January 2015 to March 2022 were involved in the study. The clinical features of these newborns and pregnancy details were compared between newborns in the survival and lethal groups. Univariable and multivariate analyses were established in sequence to demonstrate the essential risk factors. The nomogram prediction model was built.

Results

A total of 203 newborns were included in the analysis. 136 (67.0%) newborns represented the hospital survival group. Plasma pH value (OR = 0.606, p = 0.000, 95% CI 0.45715–0.80315), septicemia (OR = 3.544, p = 0.000, 95% CI 1.85160–6.78300), and abnormal pregnancy history (OR = 3.331, p = 0.008, 95% CI 1.37550–8.06680) were identified as independent risk factors for neonatal death in newborns associated with PPHN. Finally, the nomogram predictive model was established based on multivariate analysis results, indicating the efficacies of prediction and calibration.

Conclusion

This study generated an applicable risk score formula using the plasma pH value, septicemia, and abnormal pregnancy history to recognize neonatal death in newborns with PPHN, presenting a sufficient predictive value and calibration.
Interpretable cardiac anatomy modeling using variational mesh autoencoders
Authors: Beetz Marcel , Corral Acero Jorge , Banerjee Abhirup , Eitel Ingo , Zacur Ernesto , Lange Torben , Stiermaier Thomas , Evertz Ruben , Backhaus Sören J. , Thiele Holger , Bueno-Orovio Alfonso , Lamata Pablo , Schuster Andreas , Grau Vicente
Published: December 2022

Cardiac anatomy and function vary considerably across the human population with important implications for clinical diagnosis and treatment planning. Consequently, many computer-based approaches have been developed to capture this variability for a wide range of applications, including explainable cardiac disease detection and prediction, dimensionality reduction, cardiac shape analysis, and the generation of virtual heart populations. In this work, we propose a variational mesh autoencoder (mesh VAE) as a novel geometric deep learning approach to model such population-wide variations in cardiac shapes. It embeds multi-scale graph convolutions and mesh pooling layers in a hierarchical VAE framework to enable direct processing of surface mesh representations of the cardiac anatomy in an efficient manner. The proposed mesh VAE achieves low reconstruction errors on a dataset of 3D cardiac meshes from over 1,000 patients with acute myocardial infarction, with mean surface distances between input and reconstructed meshes below the underlying image resolution. We also find that it outperforms a voxelgrid-based deep learning benchmark in terms of both mean surface distance and Hausdorff distance while requiring considerably less memory. Furthermore, we explore the quality and interpretability of the mesh VAE's latent space and showcase its ability to improve the prediction of major adverse cardiac events over a clinical benchmark. Finally, we investigate the method's ability to generate realistic virtual populations of cardiac anatomies and find good alignment between the synthesized and gold standard mesh populations in terms of multiple clinical metrics.
Glycohemoglobin: A new warning strategy for non-alcoholic fatty liver disease: Study from the NHANES 2017- 2020
Authors: Hou Jiaxing , Liu Yanyu , Deng Zhen , Sun Jichun , Zhao Mingyi
Published: December 2022

Context

The development and progression of Non-alcoholic Fatty Liver Disease (NAFLD) is associated with type 2 diabetes mellitus (T2DM), but there are no studies to demonstrate whether blood glucose levels are associated with the progression of NAFLD.

Objective

Alterations in glucose metabolism may cause hepatic steatosis and inflammatory responses, leading to hepatocyte damage and promoting NAFLD’s progression. Since glycohemoglobin reflects current blood glucose levels and is easily detectable, the present study aimed to investigate whether glycohemoglobin is associated with liver stiffness in patients with NAFLD.

Methods

We studied 1510 NAFLD patients aged 20-80 in NHANES 2017- March 2020, who were defined using the controlled attenuation parameter (CAP) ≥263 dB/m. Multivariable linear regressions were used to assess the independent association between glycohemoglobin and median liver stiffness measurements (LSM) after adjusting for potential confounders. Subsequently, they were subjected to curve fitting and threshold effect analysis. Stratified analysis was used to find the variables affecting the relationship.

Results

Glycohemoglobin and median LSM showed a positive correlation in different models (β (95% CI): Crude Model: 1.460 (1.053, 1.867); Model 1: 1.476 (1.066, 1.885); Model 2: 1.517 (0.919, 2.115)), and this correlation disappeared when glycohemoglobin ≥8.6%. Furthermore, this correlation was more pronounced in the non-diabetic and former smoking subgroups.

Conclusion

In patients with NAFLD, glycohemoglobin may reflect the degree of liver stiffness, and preventing excessive glycohemoglobin may have a positive effect on slowing the progression of NAFLD especially in non-diabetic and former smokers.
Piezoelectric ceramic materials on transducer technology for energy harvesting: A review
Authors: Ogbonna V. E. , Popoola A. P. I. , Popoola O. M.
Published: December 2022

Recently, energy harvesting through the means of piezoelectric transducer technology has increasingly attracted the attention of engineers and scientists in producing/generating electricity for human consumption. However, understanding of piezoelectric materials for application in piezoelectric transducer devices in energy harvesting remains important in today’s energy systems engineering. Thus, the present review study is centered on piezoelectric materials for a better understanding of the properties of different piezoelectric materials (ceramic) when placed under mechanical stress or vibration and electrical field during energy harvesting using transducer devices. With the available literature, lead zirconate titanate materials showed to be the most common piezoelectric material with a high energy-generating performance but possessed more mechanical failure and also compromised in a harsh environment compared to lead-free piezoelectric materials. As such, the authors conclude that lead-free piezoelectric materials, such as zinc oxide and barium titanate, remain the best conducive piezoelectric material over lead zirconate titanate, which basically affects the human environment due to its toxicity. Thus, to widen the use of lead-free piezoelectric materials in energy harvesting, owing to their improved properties and environment-friendly nature, the authors recommend further enhancement of the lead-free piezoelectric material properties via nanodielectric filler incorporations using the spark plasma sintering technique.
The effectiveness of citizen surveillance for detecting exotic vertebrates
Authors: Caley Peter , Barry Simon C.
Published: December 2022

Citizen observations of the natural world are increasing in detail, growing in volume and increasingly being shared on web-based platforms for the purpose of sharing information and/or the crowd-sourcing of species identification. From a biosecurity perspective, such citizen data streams are important as they are responsible for the majority of post-border reports and most detections of exotic pest species of concern. The sharing of sightings amongst what are effectively communities of practice is a key driver of having the sighting of an exotic pest species recognized and reported. Whilst it is clear that the eyes, ears, cameras, and microphones of citizens are a major component of biosecurity surveillance, it is unclear what level of surveillance this provides in the prospective sense. As an example, what confidence does citizen science provide about “proof of absence” for exotic pests of concern? The taxonomy of surveillance used within the field of biosecurity would classify such citizen activities as contributing to “general surveillance,” for which non-detections are typically not recorded and methods of quantitative analysis are still under development. We argue that while not recorded, there is considerable information about citizens activities that routinely underpins peoples mental inference about the level of surveillance provided by citizen activities. Furthermore, we show that it is possible to make such inference from general surveillance transparent by describing and characterizing the activities that potentially generate sightings in a way that is amenable to quantitative analysis. In the context of evaluating surveillance provided by citizens for incursions of exotic vertebrates, we provide examples of citizen observations providing early warning and hence preventing the establishment of species from a range of animal groups. Historically, analysis of the power of general surveillance has been restricted to being conceptual, based on qualitative arguments. We provide this, but also provide a quantitative model framework and provide examples of how different forms of general surveillance data may be analyzed, particularly in supporting inference of eradication/extinction.
Vigorous rootstocks improve yields and increase fruit sizes in grafted fresh market tomatoes
Authors: Ingram Thomas W. , Sharpe Suzette , Trandel Marlee , Perkins-Veazie Penelope , Louws Frank J. , Meadows Inga
Published: December 2022

Grafting high yielding tomato cultivars (Solanum lycopersicum L.) onto vigorous rootstocks can increase marketable yields, but questions remain regarding optimal cultural and growing conditions such as pinching and plant spacing. This study addressed some of the dynamics between grafted plants and cultural practices. Two scions, ‘Tasti-Lee’ (TL) and ‘Mountain Fresh Plus’ (MFP) were grafted onto each of three rootstocks, ‘Beaufort’, ‘Arnold’, and ‘Shield’. Plants were pinched (removal of main shoot to induce both axillary shoots to grow) or non-pinched, and spaced at 56 or 61 cm. All 32 grafted treatments were compared to the grower standard: non-grafted TL and MFP spaced at 46 cm, which were non-pinched. Fruit quality traits including soluble solids content, pH, lycopene concentration, and titratable acidity were recorded for fruit harvested from tomatoes grafted onto ‘Arnold’, ‘Beaufort’, ‘Shield’ and non-grafted TL. The overall effect of grafting TL and MFP onto vigorous rootstocks ‘Arnold’, ‘Beaufort’, and ‘Fortamino’ increased marketable yields per hectare by 24-35% compared to non-grafted grower standards. The rootstock ‘Shield’ did not significantly increase yields with either scion. TL had a more positive response to grafting than MFP. ‘Arnold’, ‘Beaufort’, and ‘Fortamino’ significantly increased TL fruit size, but the fruit size results were not as significantly impacted by graft treatments for MFP. Plants spaced at 56 compared to 61 cm generated similar yields. Pinched plants significantly increased yields over non-pinched plants by 15% in 2018 but did not impact yield in 2017. No consistent difference was observed between pinched and non-pinched plants with regard to fruit size, only plants grafted with ‘Shield’ benefited significantly from being pinched. Soluble solids content, pH, total lycopene concentration, and titratable acidity differed slightly between grafted and non-grafted plants but was unlikely to positively or negatively affect overall perception of fruit quality in tomatoes. A partial budget analysis revealed that grafting with ‘Arnold’ or ‘Beaufort’ consistently increased profits in this low-disease field.
New insights into the analgesic properties of the XCL1/XCR1 and XCL1/ITGA9 axes modulation under neuropathic pain conditions - evidence from animal studies
Authors: Ciechanowska Agata , Rojewska Ewelina , Piotrowska Anna , Barut Justyna , Pawlik Katarzyna , Ciapała Katarzyna , Kreiner Grzegorz , Mika Joanna
Published: December 2022

Recent studies have indicated the involvement of chemokine-C-motif ligand 1 (XCL1) in nociceptive transmission; however, the participation of its two receptors, canonical chemokine-C-motif receptor 1 (XCR1) and integrin alpha-9 (ITGA9), recently recognized as a second receptor, has not been clarified to date. The aim was to explore by which of these receptors XCL1 reveals its pronociceptive properties and how the XCL1-XCR1 and XCL1-ITGA9 axes blockade/neutralization influence on pain-related behavior and opioid analgesia in the model of neuropathic pain. In our studies we used Albino Swiss mice which were exposed to the unilateral sciatic nerve chronic constriction injury (CCI) as a neuropathic pain model. Animals received single intrathecal (i.t.) injection of XCL1, XCL1 neutralizing antibodies, antagonist of XCR1 (vMIP-II) and neutralizing antibodies of ITGA9 (YA4), using lumbar puncture technique. Additionally we performed i.t. co-administration of abovementioned neutralizing antibodies and antagonists with single dose of morphine/buprenorphine. To assess pain-related behavior the von Frey and cold plate tests were used. To measure mRNA and protein level the RT-qPCR and Western Blot/Elisa/immunofluorescence techniques were performed, respectively. Statistical analysis was conducted using ANOVA with a Bonferroni correction. Presented studies have shown time-dependent upregulation of the mRNA and/or protein expression of XCL1 in the spinal cord after nerve injury as measured on day 1, 4, 7, 14, and 35. Our immunofluorescence study showed that XCL1 is released by astroglial cells located in the spinal cord, despite the neural localization of its receptors. Our results also provided the first evidence that the blockade/neutralization of both receptors, XCR1 and ITGA9, reversed hypersensitivity after intrathecal XCL1 administration in naive mice; however, neutralization of ITGA9 was more effective. In addition, the results proved that the XCL1 neutralizing antibody and, similarly, the blockade of XCR1 and neutralization of ITGA9 diminished thermal and mechanical hypersensitivity in nerve injury-exposed mice after 7 days. Additionally, neutralization of XCL1 improves morphine analgesia. Moreover, blockade of XCR1 positively influences buprenorphine effectiveness, and neutralization of ITGA9 enhances not only buprenorphine but also morphine analgesia. Therefore, blockade of the XCL1-ITGA9 interaction may serve as an innovative strategy for the polypharmacotherapy of neuropathic pain in combination with opioids.
Inflammatory checkpoints in amyotrophic lateral sclerosis: From biomarkers to therapeutic targets
Authors: Jiang Zongzhi , Wang Ziyi , Wei Xiaojing , Yu Xue-Fan
Published: December 2022

Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disease characterized by progressive motor neuron damage. Due to the complexity of the ALS, so far the etiology and underlying pathogenesis of sporadic ALS are not completely understood. Recently, many studies have emphasized the role of inflammatory networks, which are comprised of various inflammatory molecules and proteins in the pathogenesis of ALS. Inflammatory molecules and proteins may be used as independent predictors of patient survival and might be used in patient stratification and in evaluating the therapeutic response in clinical trials. This review article describes the latest advances in various inflammatory markers in ALS and its animal models. In particular, this review discusses the role of inflammatory molecule markers in the pathogenesis of the disease and their relationship with clinical parameters. We also highlight the advantages and disadvantages of applying inflammatory markers in clinical manifestations, animal studies, and drug clinical trials. Further, we summarize the potential application of some inflammatory biomarkers as new therapeutic targets and therapeutic strategies, which would perhaps expand the therapeutic interventions for ALS.
NR4A2 may be a potential diagnostic biomarker for myocardial infarction: A comprehensive bioinformatics analysis and experimental validation
Authors: Wei Dongsheng , Qi Jiajie , Wang Yuxuan , Li Luzhen , Yang Guanlin , He Xinyong , Zhang Zhe
Published: December 2022

Background

Myocardial infarction is a well-established severe consequence of coronary artery disease. However, the lack of effective early biomarkers accounts for the lag time before clinical diagnosis of myocardial infarction. The present study aimed to predict critical genes for the diagnosis of MI by immune infiltration analysis and establish a nomogram.

Methods

Gene microarray data were downloaded from Gene Expression Omnibus (GEO). Differential expression analysis, single-cell sequencing, and disease ontology (DO) enrichment analysis were performed to determine the distribution of Differentially Expressed Genes (DEGs) in cell subpopulations and their correlation with MI. Next, the level of infiltration of 16 immune cells and immune functions and their hub genes were analyzed using a Single-sample Gene Set Enrichment Analysis (ssGSEA). In addition, the accuracy of critical markers for the diagnosis of MI was subsequently assessed using receiver operating characteristic curves (ROC). One datasets were used to test the accuracy of the model. Finally, the genes with the most diagnostic value for MI were screened and experimentally validated.

Results

335 DEGs were identified in GSE66360, including 280 upregulated and 55 downregulated genes. Single-cell sequencing results demonstrated that DEGs were mainly distributed in endothelial cells. DO enrichment analysis suggested that DEGs were highly correlated with MI. In the MI population, macrophages, neutrophils, CCR, and Parainflammation were significantly upregulated compared to the average population. NR4A2 was identified as the gene with the most significant diagnostic value in the immune scoring and diagnostic model. 191 possible drugs for the treatment of myocardial infarction were identified by drug prediction analysis. Finally, our results were validated by Real-time Quantitativepolymerase chain reaction and Western Blot of animal samples.

Conclusion

Our comprehensive in silico analysis revealed that NR4A2 has huge prospects for application in diagnosing patients with MI.
Platelet abnormalities in autoimmune thyroid diseases: A systematic review and meta-analysis
Authors: Cao Yu-tian , Zhang Kai-yu , Sun Jing , Lou Yan , Lv Tian-su , Yang Xinyi , Zhang Wen-hui , Yu Jiang-yi , Wu Qi-biao , Zhou Xi-qiao
Published: December 2022

Background

Some degree of platelet index abnormality has been found clinically in the autoimmune thyroid disease (AITD), but the findings are not uniform.

Methods

The PubMed, Web of Science, Cochrane Library, and Embase databases were searched for relevant articles published up to August 16th, 2022, with no restrictions on the language of the articles. Reference lists of eligible articles were also searched. A random effect model was used to pool the standardized mean difference (SMD) and 95% confidence interval (95% CI) of platelet count (PLT), mean platelet volume (MPV), and platelet distribution width (PDW) between AITD patients and healthy controls, and subgroup analyses were performed.

Results

A total of 19 articles with 6173 people (3824 AITD patients and 2349 healthy people) were included in the meta-analysis. The results showed that PLT and MPV values were significantly increased in AITD patients when compared with healthy people (SMD: 0.164, 95% CI: 0.044 to 0.285; SMD: 0.256, 95% CI: 0.013 to 0.500), while no significant difference was found in PDW between the AITD group and the control group (SMD: 0.060, 95% CI: -0.164 to 0.284). Subgroup analysis according to disease type and thyroid function revealed that for PLT, this difference was only found in the Hashimoto’s thyroiditis (HT) and hypothyroid groups, but not in the Graves’ disease (GD) and hyperthyroid groups. For MPV, the results were the opposite of those for PLT: MPV was significantly higher in the GD, hyperthyroid, and euthyroid groups than in the control group, but not in the HT and hypothyroid groups. Sensitivity analysis showed that the stability of the pooled MPV was not good. No publication bias was found.

Conclusions

PLT and MPV are significantly elevated in patients with AITD, with PLT being more significantly elevated in HT and hypothyroidism, and MPV being more significantly increased in GD and hyperthyroidism. Appropriate clinical attention can be paid to the thyroid function of patients when abnormal platelet indices are found, and conversely, the consequences of abnormal platelet parameters such as elevated MPV lead to an increased occurrence of cardiovascular events, which should also be addressed in the AITD population.

Systematic review registration

https://www.crd.york.ac.uk/PROSPERO/, identifier CRD42022341823.
Promoting human rights for Taiwan’s fishermen: Collaboration with the primary source countries of Taiwan’s DWF migrant fishermen
Authors: Hung Po-Chih , Lee Hsiao-Chien , Lin Chih-Cheng , Liu Wen-Hong
Published: December 2022

Taiwan is one of the largest distant water fishing (DWF) nations worldwide, and relies largely on the migrant labor to keep costs low. However, this industry has caused Taiwan to be listed in the 2020 “List of Goods Produced by Child Labor or Forced Labor” of the U.S. Department of Labor. In view of this, the Taiwanese government is actively adopting further management measures to supervise the domestic and foreign fishermen agencies. It is because the latter has been involved in many disputes, especially in recruitment, payroll, and labor contracts, which directly or indirectly affect the rights of migrant fishermen. On the other hand, although the C188 Work in Fishing Convention has stregthend the protection of the fishermen’s human rights, it still stays ambiguous in terms of private agency management. That is also why so many disputes have been caused in recent years.This study conducts a comparative analysis of the agency management systems in the primary source countries of Taiwan’s distant water fishing migrant fishermen (that is, Indonesia, the Philippines, and Vietnam), as well as interviews with distant water fishing stakeholders to provide insights on the improvement of agency management and migrant fishermen’s rights in Taiwan. The findings imply that the positive interaction, mutual trust, and understanding of laws and regulations between fishermen’s exporting and importing countries lead to future cross-national collaboration. This study suggests that the Taiwanese government should follow the spirit of the C188 but not be restricted to the Convention texts to amend or formulate regulations and policies of agencies for fully protecting the rights of migrant fishermen.
Case report: Emphysematous pyelonephritis associated with kidney allograft abscess formation
Authors: Abu Jawdeh Bassam G. , Nguyen Michelle C. , Ryan Margaret S. , Vikram Holenarasipur R.
Published: December 2022

Emphysematous pyelonephritis (EPN) is a severe, acute necrotizing infection that is defined by the presence of gas in the kidney parenchyma. Multiple case reports have described the radiological findings and clinical course of EPN. Herein, we report on EPN including the histopathological findings in a kidney transplant recipient. Our patient presented with EPN complicated by multiorgan failure and was successfully managed with transplant nephrectomy.
Case report: BRAF-inhibitor therapy in BRAF-mutated primary CNS tumours including one case of BRAF-mutated Rosai-Dorfman disease
Authors: Cronin Christopher , McLaughlin Ronan , Lane Louise , Brett Francesca M. , Jansen Michael , Bermingham Niamh , Wyse Gerald , Grogan Liam , Morris Patrick G. , O’Reilly Seamus
Published: December 2022

BRAF V600E oncogene mutations have been reported in multiple central nervous system (CNS) tumor types, and emerging evidence supports the use of targeted therapy in BRAF-mutated gliomas. BRAF oncogene mutations have been recently identified in Rosai-Dorfman disease (RDD)—a rare non-Langerhans cell histiocytosis. This series describes three patients from two neurosurgical centers in Ireland with BRAF V600E-mutated CNS tumors. The study participants include a 19-year-old male patient with ganglioglioma with anaplastic features, a 21-year-old male patient with CNS involvement of RDD, and a 28-year-old female patient with ganglioglioma with anaplastic features. Two patients received radiation with concurrent temozolomide before BRAF-targeted therapy. This case series describes clinical and radiological responses to BRAF-targeted therapy in BRAF V600E-mutated gliomas across multiple tumor grades and is only the second published report of response to targeted therapy in BRAF-mutated RDD. The durability of disease control with BRAF-targeted therapy was generally superior to that achieved with chemoradiation; one patient has experienced ongoing disease control for 5 years. The reported case of treatment response in BRAF-mutated RDD supports the strategy of genotyping and utilization of targeted therapy in this rare disease. The optimal sequencing of BRAF-targeted therapy in BRAF-mutated gliomas/glioneuronal tumors remains unclear, and further prospective studies are required to guide the use of genome-matched therapy in this patient population.
Valorization as a biofertilizer of an agricultural residue leachate: Metagenomic characterization and growth promotion test by PGPB in the forage plant Medicago sativa (alfalfa)
Authors: Robas Mora Marina , Fernández Pastrana Vanesa M. , Probanza Lobo Agustín , Jiménez Gómez Pedro A.
Published: December 2022

The abuse of chemical fertilizers in intensive agriculture has turned out in the contamination of ground and the soil on which they are applied. Likewise, the generation, storage, and destruction of plant residues from the agri-food industry poses a threat to the environment and human health. The current situation of growing demand for food implies the urgent need to find sustainable alternatives to chemical fertilizers and the management of agricultural waste. Valorization of this plant residue to produce natural biofertilizers using microbiological treatments is presented as a sustainable alternative. The microbial activity allows the transformation into simple molecules that are easily absorbed by plants, as well as the stimulation of plant growth. This double direct and indirect action induced significant increases against the variables of germination, viability, and biomass (dry weight). To guarantee biosafety, it is necessary to use new bio-technological tools, such as metagenomics, which allow the taxonomic analysis of microbial communities, detecting the absence of pathogens. In the present paper, a physicochemical and metagenomic characterization of a fertilizer obtained from agricultural plant waste valorization is carried out. Likewise, fertigation treatments were tested to which the Plant Growth Promoting Bacteria (PGPB) Pseudomonas agronomica and Bacillus pretiosus were added, both independently and in consortium. Metagenomic analysis has identified taxa belonging to the kingdoms Bacteria and Archaea; 10 phyla, 25 families, 32 genera and 34 species, none of them previously described as pathogenic. A 1/512 dilution of the fertilizer increased the germination rate of Medicago sativa (alfalfa) by 16% at 144 h, compared to the treatment without fertilizer. Both the fertilizer and the addition of PGPB in a double direct and indirect action induced significant increases against the variables of germination, viability, and biomass (dry weight). Therefore, the use of an agricultural residue is proposed, which after the addition of two new species is transformed into a biofertilizer that significantly induces plant growth in Mendicago sativa plants.
Microbiota of preterm infant develops over time along with the first teeth eruption
Authors: Zhang Yu , Wu Yi-Pei , Feng Vivien , Cao Gui-Zhi , Feng Xi-Ping , Chen Xi
Published: December 2022

Objective

The temporal growth of the infant microbiome in the early years of life influences short- and long-term infant health. The aim of this longitudinal study was to investigate bacterial dynamics in the microbiome of preterm infants during tooth eruption.

Methods

Saliva samples from normally delivered (n = 24) and preterm infants (n = 31) were collected 30 days after birth and after the eruption of two primary mandibular incisors. Based on Illumina MiSeq Sequencing of the 16S rRNA gene, the dynamic microbial changes of newborns at two-time points were investigated. Meanwhile, the Human Oral Microbiome Database was adopted for assigning taxonomy.

Results

Using alpha and beta diversity analyses, different shift patterns of microbiome structures in preterm and healthy participants and bacterial diversity over time were observed. The relative abundance and shifts trend, along with the two lower primary central incisors eruption, of core oral flora varies in full-term and preterm groups, including Gemella spp., Rothia mucilaginosa, Veillonella atypica, etc. Several microorganisms colonize later in the oral microbiome development of premature babies, such as Gemella spp. In addition to teeth eruption, the growth of the saliva microbiome in preterm infants could be influenced by breastfeeding durations and birth weight.

Conclusion

This study provided insights into how the oral microbiota changes during tooth eruption in preterm infants and how the colonization of the oral cavity with bacteria in preterm infants differs significantly from that in full-term infants.
Recent advances and trends of trichloroethylene biodegradation: A critical review
Authors: Wu Zhineng , Man Quanli , Niu Hanyu , Lyu Honghong , Song Haokun , Li Rongji , Ren Gengbo , Zhu Fujie , Peng Chu , Li Benhang , Ma Xiaodong
Published: December 2022

Trichloroethylene (TCE) is a ubiquitous chlorinated aliphatic hydrocarbon (CAH) in the environment, which is a Group 1 carcinogen with negative impacts on human health and ecosystems. Based on a series of recent advances, the environmental behavior and biodegradation process on TCE biodegradation need to be reviewed systematically. Four main biodegradation processes leading to TCE biodegradation by isolated bacteria and mixed cultures are anaerobic reductive dechlorination, anaerobic cometabolic reductive dichlorination, aerobic co-metabolism, and aerobic direct oxidation. More attention has been paid to the aerobic co-metabolism of TCE. Laboratory and field studies have demonstrated that bacterial isolates or mixed cultures containing Dehalococcoides or Dehalogenimonas can catalyze reductive dechlorination of TCE to ethene. The mechanisms, pathways, and enzymes of TCE biodegradation were reviewed, and the factors affecting the biodegradation process were discussed. Besides, the research progress on material-mediated enhanced biodegradation technologies of TCE through the combination of zero-valent iron (ZVI) or biochar with microorganisms was introduced. Furthermore, we reviewed the current research on TCE biodegradation in field applications, and finally provided the development prospects of TCE biodegradation based on the existing challenges. We hope that this review will provide guidance and specific recommendations for future studies on CAHs biodegradation in laboratory and field applications.