bluuebunny/crossref_metadata_2025 huggingface dataset:
Sprouts consumption: A correlational study between Brazilian consumers' perception, motivation to eat new products levels and food choice factors
Authors: Miyahira Roberta Fontanive , Giangiarulo Thamires , de Oliveira Lopes Jean , da Silva Wanessa Pires , Esmerino Erick Almeida , Antunes Adriane Elisabete Costa
Published: April 2023

Encouraging the consumption of natural foods with a high content of nutrients such as sprouts is a strategy that meets the nutritional guidelines of most countries. Considering that data on sprouts consumption in Brazil are scarce, the present study aimed to evaluate the perception of Brazilian participants about sprouts, correlating these data with the multidimensionality of food choice and motivations for novelties. The perception of 315 participants was evaluated, considering the Food Choice Questionnaire (FCQ), Motivation to Eat New Foods scale, and the projective technique of Word Association (WA). Based on FCQ data, participants were subdivided into two distinct clusters with specific profiles. All factors' scores related to food choice showed significantly higher scores in Group 2. Sensory appeal was their most relevant factor. Both groups were highly motivated to approach new foods. In the WA task, the most mentioned categories were associated with product, health, and environmental aspects.

Practical applications

The evaluation of consumers' perceptions about sprouts demonstrated that the inclusion of sprouts as part of the diet is feasible and aligns with the demands and motivations of Brazilian participants. As a practical application, it is suggested that strategies be developed to promote the consumption of sprouts based on sensory and nutritional aspects, as well as their relationship with health and the environment.
Other options to resolve patent infringement dispute, experiences from China
Authors: Biyu Ma , Dingming Yu
Published: April 2023

While civil litigation (CL) was expected to play a major role in resolving patent infringement disputes, administrative adjudication (AA) has become the preferred channel for patentees to protect their rights in China since 2015. The number of AA cases significantly increased during the study period of 2010–2021. Our research finds that AA does not particularly favor patentees. By comparing the rules of CL and AA, we also find that low cost and high efficiency are the two major advantages of AA. Besides these traits, the motivations for patentees to initiate AA are the lack of risks in AA procedure and its valuable outcomes. Understanding the real motivation for the application of AA could be a reference for improving the patent infringement dispute system.
Philosophical virtues
Authors: Cassam Quassim
Published: April 2023

It has been suggested that philosophers should adopt a methodology largely inspired by mathematics and that the “mathematical” virtues of rigor, clarity, and precision are also fundamental philosophical virtues. In reply, this paper argues that some excellent philosophy lacks these virtues and that too much emphasis on the mathematical virtues excludes potentially valuable forms of philosophical discourse and makes the profession less diverse than it should be. Unduly restrictive conceptions of philosophical argumentation should be avoided. On a contributory conception, philosophy should try to make a positive contribution to human emancipation where possible. The paper argues that it is possible and desirable for epistemology to contribute in this way and that the mathematical virtues are less significant in this context than the emancipatory virtues of what one might call “liberation philosophy.” These include irony, reflectiveness, imagination, contrarianism, and worldliness.
Rapid detection of dermatophytes using the Seegene Novaplex™ dermatophyte assay
Authors: van Herk Aline , Houwen Cindy , den Reijer Martijn , Beek Marianne Noordanus‐van , Niehuis Marie‐Jean , van Lisdonk Nico , Mertens Kelly , Thai Khoa T. D.
Published: April 2023

Objectives

We assessed the performance of the Seegene Novaplex™ Dermatophyte Assay for diagnosis of dermatophytosis.

Methods

Sixty‐one clinical samples from skin, nail, hair and culture were selected based on RT‐PCR according to Wisselink et al. Of these samples, 26 samples were negative and 35 samples were positive with 39 dermatophytes strains. Emerging fungal strains harbouring terbinafine resistance (i.e. T. indotineae and T. mentagrophytes) were included.

Results

The specificities of the Novaplex™ Dermatophyte Assay ranged between 94.3% and 97.9%. The sensitivities for the detection of T. rubrum complex, T. mentagrophytes/T. interdigitale species complex and C. albicans were 94.1% (95% CI: 71.3–99.9), 78.6% (95% CI: 49.2–95.3) and 100% (95% CI: 69.2–100), respectively, with Cohen's kappa of at least 72.9%.

Conclusions

The Seegene Novaplex™ Dermatophyte Assay can be used for reliable screening of dermatophytes, including emerging strains in a routine laboratory setting.
The concept of the Hausa nation from an ethnolinguistic perspective
Authors: Pawlak Nina
Published: April 2023

The paper investigates the concept of the Hausa nation from a linguistic perspective. Using ethnolinguistic methodology, which enables the study of the relationship between language and culture, it focuses on the encoding of the NATION (as well as the similar concept HOMELAND) in the Hausa language. Phrases, words, and expressions demonstrate how the idea is perceived by the community and how the concept is reflected in the ‘linguistic worldview’. Attention is paid to the terms that are cultural keywords, petrified phrases and proverbs that manifest the patterns of national identification. The paper concludes that the concept of ‘nation’ in Hausa is encoded with reference to the national community, which has no state of its own. According to the manner in which it is conceived, two ethnolinguistic profiles of the homeland can be identified: one motivated by ethnic (language) identity; the other by state (Nigerian) identity.
Characterization of the novel HLA‐DPA1*01:144 allele by next generation sequencing
Authors: Liacini Abdelhamid , Peters Lindsey , Mancini Shannon , Persidsky Yuri , Geier Steven
Published: April 2023

HLA‐DPA1*01:144 differs from HLA‐DPA*01:03:01:04 by one nucleotide substitution at position 44, codon‐17 located in exon 1.
Community‐engaged primary care medical education
Authors: Harding Daniel , Uppal Sheila , Meiring Samantha , Golding Bethany , Dutta Nina , Parekh Ravi
Published: April 2023

Background

Community‐engaged medical education (CEME) requires medical schools to partner with local communities to help address community priorities, whilst enhancing the learning experiences of students. Current literature on CEME has focused on evaluating its effects on students; however, there remains a gap in exploring whether CEME initiatives can have a sustainable impact for communities.

Approach

The Community Action Project (CAP) at Imperial College London, is an eight‐week, community‐engaged, quality improvement project for Year 3 medical students. Students initially consult with clinicians, patients and wider community stakeholders to understand local needs and assets, and identify a health priority to address. They then work with relevant stakeholders to design, implement and evaluate a project to help address their identified priority.

Evaluation

All CAPs (n = 264) completed in the 2019–2021 academic years were evaluated for evidence of several key areas, including community engagement and sustainability. 91% of projects evidenced a needs analysis, 71% demonstrated patient involvement in their development, and 64% demonstrated sustainable impacts from their projects. Analysis revealed the topics frequently addressed, and the formats used by students. Two CAPs are described in more detail to demonstrate their community impact.

Implications

The CAP demonstrates how the principles of CEME (meaningful community engagement and social accountability) can lead to sustainable benefits for local communities through purposeful collaboration with patients and local communities. Strengths, limitations and future directions are highlighted.
The Stigmatization of Trauma: A Reflection on Mahesh Dattani's Select Works
Authors: A Thamizh Magal , V Sunitha
Published: April 2023

Trauma is a response to an experience that devastates a person mentally. It frequently causes shock, denial, and changes in the body, mind, and behavior. People witness difficult circumstances and long-lasting repercussions on their psychological and physical well-being due to trauma. The chosen plays Tara and 30 Days in September are examined using trauma theory. The traumatic stigma of victimized characters and their offenders are explored and brought to light in the present study. The paper maps out the common aspects of trauma in Mahesh Dattani's plays and creates a portal for scholarly reflections on the author's ideology of trauma. It attempts to explore the reason behind the abnormal behavior of the characters and argues that childhood plays a major role in shaping the behavioral attributes of the adults. The researcher has made thematic analysis on the select plays through attentive reading. The study aims to develop an in-depth understanding on the effects of post-traumatic stress disorder on the characters taken for study and the possible ways to liberate from traumatic disorder. It strives to cease the stigmatization of trauma and emphasizes the fact that proper counseling, care and support will help the trauma affected characters to recover from the disorder.
Exploring Factors That Impact on Motivation in Foreign Language Learning in the Classroom
Authors: Mochklas Mochamad , Ngongo Magdalena , Sianipar Martin Yehezkiel , Kizi Sobirova Nurkhon Barot , Putra Randi Eka , Al-Awawdeh Nabil
Published: April 2023

In the study of second language learning, one of the most critical components is unveiling factors that impact on motivation in the classroom. Proficiency in foreign language learning is inextricably tied to motivation, in the understanding that proficiency may not take place if the learners are not strategically motivated. The effectiveness of an L2 learners’ growth may be dependent on various factors that affect motivation in the classroom. This study is an analysis of different factors that influence motivation in foreign language classroom. The study examined how different activities and situations influence the motivation of foreign language learners in the classroom. A total of 119 persons participated in the study, including high school teachers and their students. Questionnaire was used to collect the needed data, and data was analyzed using tables and graphs to discuss the factors that affect motivation in the classroom. Teaching strategy and style of learning, feedback, reward, group, class environment, technology, and punishment are the factors measured. The findings of the study indicate that teaching strategy and learning style exert huge impact on motivation, with over 39.50% of the response rate. This is closely followed by feedback and reward with about 19.32% and 12.60% respectively. The study further revealed that group has the least influence on motivation, with 2.52% value, which is lower than the impact of class environment that stands at 7.56%. It is concluded that both learners and teachers of foreign language accept that teaching strategy and learning style is the most significant factor that impacts on motivation. It is recommended for further studies to use larger study sample from different schools to explore these factors and ascertain their degree of impact on motivation in foreign language teaching and learning.
Complete Genome Sequence of the First Colistin-Resistant Raoultella electrica Strain
Authors: Aldeia Claudia , Campos-Madueno Edgar I. , Sendi Parham , Endimiani Andrea
Published: April 2023

We present the full genome sequence of a colistin-resistant
Raoultella electrica
strain (MIC, >4 μg/mL) that was isolated from the stool of a healthy person living in India. The sequence consists of a chromosome and three plasmids (5,455,992-bp and 98,913-bp, 4,232-bp, and 3,961-bp, respectively). No previously described colistin resistance mechanisms were detected.
Draft Genome Sequences of 18
Bacteroidetes
Strains Isolated from a Human Stool Sample
Authors: Yousuf Basit , Mottawea Walid , Bouhlel Nour Elhouda , Hammami Riadh
Published: April 2023

We announce the draft genome sequences of 12
Bacteroides
, 4
Phocaeicola
, and 2
Parabacteroides
strains, among which was a newly isolated species,
Bacteroidaceae
bacterium UO.H1004. These isolates produce health-benefiting short-chain fatty acids (SCFAs) and the neurotransmitter γ-aminobutyric acid (GABA) in various concentrations.
Supplemental Material: Two episodes of late Mesoproterozoic magmatism in the southwestern Yangtze Block, China, and their association with Grenville-aged orogenic cycles
Authors: Wang Yan-Jun , zhu wei-guang , al. et
Published: April 2023

 Figure S1: Representative field photos and microscopic photographs of mafic rocks in this study. Figure S2: Plot of whole-rock εNd(t) versus age (Ga) for the Boka dolerites, Lanniping gabbros, and Tong’an dikes. Figure S3: Covariations of the high field strength elements (e.g., TiO2, Th, Nb, Nd, Eu, Yb), compatible element (e.g., Ni), and large ion lithophile elements (e.g., Ba and Sr) relative to Zr contents for the Boka dolerites, Lanniping gabbros, and Tong’an dikes. Figure S4: Covariations of SiO2, TiO2, Fe2O3T, CaO, Al2O3, CaO/Al2O3, P2O5, Ni, and Cr relative to MgO contents for the Boka dolerites, Lanniping gabbros, and Tong’an dikes. Figure S5: Th/Nb versus TiO2/Yb plot for the late Mesoproterozoic mafic rocks in the southwestern Yangtze Block. Table S1: Cameca secondary ion mass spectrometry (SIMS) zircon and baddeleyite U-Pb isotopic analyses for the late Mesoproterozoic mafic rocks from the Huili-Dongchuan area, southwestern Yangtze Block. Table S2: Major-element (wt%) and trace-element (ppm) data of the late Mesoproterozoic mafic rocks from the southwestern Yangtze Block. Table S3: Sm-Nd isotopic compositions of the late Mesoproterozoic mafic rocks from the southwestern Yangtze Block. 
Hypereosinophilic syndrome presenting with gastrointestinal manifestations
Authors: Hinkle Louis , Gandhi Nisarg , Neal Ryan , Narcisse Victor
Published: April 2023

Hypereosinophilic syndrome (HES) is a spectrum of diseases characterised by an elevated eosinophilic count causing end-organ damage. Differential diagnoses of hypereosinophilia are vast and include drug hypersensitivities, allergies, infections, cancers, autoimmune disorders and rare eosinophilic syndromes. Herein, we describe a case of a patient presenting with gastrointestinal (GI) symptoms including progressive dysphagia, abdominal distension, vomiting, diarrhoea and abdominal pain with significant peripheral eosinophilia who was found to have an overlap HES involving the GI tract. This patient’s eosinophilia was rapidly corrected with intravenous methylprednisolone, and the patient experienced gradual resolution of clinical symptoms with maintenance oral prednisone. Due to the rarity and diverse presentation of HES, there are few large, longitudinal studies that describe disease progression and inform treatment guidelines. This case demonstrates the difficulty in designing a treatment regimen for these patients and emphasises the clinical need for improved understanding of HES.
Modelling admission lengths within psychiatric intensive care units
Authors: Dye Stephen , Sethi Faisil , Kearney Thomas , Rose Elizabeth , Penfold Leia , Campbell Malcolm , Valsraj Koravangattu
Published: April 2023

Objectives

To examine whether discharge destination is a useful predictor variable for the length of admission within psychiatric intensive care units (PICUs).

Methods

A clinician-led process separated PICU admissions by discharge destination into three types and suggested other possible variables associated with length of stay. Subsequently, a retrospective study gathered proposed predictor variable data from a total of 368 admissions from four PICUs. Bayesian models were developed and analysed.

Results

Clinical patient-type grouping by discharge destination displayed better intraclass correlation (0.37) than any other predictor variable (next highest was the specific PICU to which a patient was admitted (0.0585)). Patients who were transferred to further secure care had the longest PICU admission length. The best model included both patient type (discharge destination) and unit as well as an interaction between those variables.

Discussion

Patient typing based on clinical pathways shows better predictive ability of admission length than clinical diagnosis or a specific tool that was developed to identify patient needs. Modelling admission lengths in a Bayesian fashion could be expanded and be useful within service planning and monitoring for groups of patients.

Conclusion

Variables previously proposed to be associated with patient need did not predict PICU admission length. Of the proposed predictor variables, grouping patients by discharge destination contributed the most to length of stay in four different PICUs.
Analysis of povidone iodine, chlorhexidine acetate and polyhexamethylene biguanide as wound disinfectants: in vitro cytotoxicity and antibacterial activity
Authors: Zhang Mingshi , Jin Jian , Liu Yingying , Ben Chi , Li Haihang , Cheng Dasheng , Sun Yu , Guang-Yi Wang , Zhu Shihui
Published: April 2023

Objectives

Even though disinfectants are commonly used in clinical practice and daily life, there are few studies on their antibacterial ability and cytotoxicity, which are closely related to the safety and effectiveness of their use. To provide a basis for the use of disinfectants, the cytotoxicity and antibacterial activity of three most commonly used disinfectants, povidone-iodine, chlorhexidine acetate and polyhexamethylene biguanide (PHMB), were investigated.

Design

A CCK-8 assay was used to measure the activities of human fibroblasts (HF) and keratinocytes (HaCat), the two most important cells in wound healing, following their exposure to disinfectants. The effects of different times and concentrations were included. The antibacterial activity of disinfectants againstStaphylococcus aureus, Acinetobacter baumannii, Klebsiella pneumoniaewas reflected by their minimum inhibitory concentration and minimum bactericidal concentration.

Results

All three disinfectants showed strong cytotoxicity in direct contact with HF and HaCat cells. Cytotoxicity increased with increasing exposure time and concentration.S. aureus,A. baumannii and K. pneumoniaecomprised 70%, 55% and 85% of the strains sensitive to povidone iodine; 50%, 45% and 80% of the strains sensitive to chlorhexidine acetate; and 60%, 45% and 80% of the strains sensitive to PHMB, respectively.

Conclusions

All three disinfectants were cytotoxic; therefore, it is necessary to pay attention to the use time and concentration in the clinical setting. All three disinfectants were cytotoxic, with povidone-iodine being the most cytotoxic even at low concentrations. PHMB had better antibacterial efficacy againstS. aureusand is suitable for the treatment of shallow wounds primarily. All three tested bacteria were significantly more sensitive to PHMB than to the other disinfectants.
Estimation of Urban Green Cover Changes in Colombo Municipal Area over Ten Years Using Remote Sensing Techniques
Authors: ATAPATTU Karunarathna Atapattu Mudiyanselage Ruwanmali Priyanwada , EDIRISINGHE E. A. Nadeeka M. , NISSANKA Sarath P. , FERNANDO T. Tamasha , GUNAWARDENA Ajith Rohana
Published: April 2023

Colombo is the commercial capital of Sri Lanka, with a high population, buildings, and vehicles. Therefore, it is vital to observe the spatial distribution of vegetation types and changes in the green cover of Colombo city to identify priority areas to improve green cover. This study was carried out to estimate the changes in green cover in Colombo Municipal Area (CMA) and its postal zones over 10 years using remote sensing techniques. The green cover was categorized into trees, shrubs, Playgrounds (PG)/Grasslands, wetlands, rooftops, and Ipomoea cover. Accordingly, total green cover increased up to 26.17% from 22.36% during the period of 2012–2022. During the past decade, except for the PG/Grasslands, all other vegetation types have been reduced. Five of the 15 postal zones in the CMA’s green cover have decreased during the past 10 years, including Colombo 04, 05, 06, 09, and 13. The highest green cover was recorded in Colombo 07, while the lowest green cover was present in Colombo 11. The outcome of the study emphasizes that CMA is moving towards greening and sustainability even with the expansion of built-up areas and urban populations.
Factors Predicting Compliance with the Uptake of the Third COVID-19 Vaccine among the Arab Minority in Israel
Authors: Ali-Saleh Ola , Khatib Mohammad , Hadid Salam
Published: April 2023

Between December 2020 and February 2021, Israel administered two doses of COVID-19 vaccine to >50% of its adult population. Nonetheless, due to declining immunity and the spread of the COVID-19 delta variant, the government offered a third COVID-19 vaccine dose in July 2021. Although the vaccine was shown to provide effective protection against severe illness, the compliance rate among the Arab minority remained low. The present study sought to identify the factors predicting willingness to uptake the COVID-19 vaccine in the Arab community. An online survey of 2486 participants was conducted in November 2021 to assess vaccination-related behaviours, such as perceived benefits, barriers, incentives (e.g., the green pass), subjective norms, and pandemic fatigue. Positive correlations were found among reasons for obtaining a green pass, trust in formal authorities, perceived effectiveness of the third COVID-19 vaccine dose, subjective norms, and attitudes toward the booster dose. Pandemic fatigue was positively correlated with vaccination barriers. Trust in authorities, perceived booster dose effectiveness, subjective norms, and attitudes were negatively related to pandemic fatigue and barriers to vaccination. Demographic variables such as age, education level, and income level were positively related to odds of getting the booster. Participants who reported being religious exhibited a lower rate of booster dose compliance than secular participants. The study findings show that the reasons for acquiring a green pass were positively correlated with the perceived effectiveness of the booster dose, demonstrating that individuals understood the vaccine benefits. Further, having a green pass was negatively related to barriers. Incentives such as the green pass play a major role in encouraging the population to take the COVID-19 vaccine. In addition, public campaigns to explain the health benefits and refute erroneous myths support higher vaccination rates.
Identification of a Novel Astrovirus Associated with Bovine Respiratory Disease
Authors: Nelsen April , Knudsen David , Hause Ben M.
Published: April 2023

Astroviruses (AstVs) cause gastrointestinal disease in mammals and avians. Emerging evidence suggests that some AstVs have extraintestinal tissue tropism, with AstVs detected in the liver, kidney, central nervous system, and the respiratory tract variably associated with disease. In cattle, AstV infection has been linked to gastroenteric or neurologic disease. Here, metagenomic sequencing of a lung from a bovine with respiratory disease identified a novel AstV with a predicted capsid-encoding ORF2 amino acid sequence with 66% identity to caprine astrovirus (CAstV G2.1). A quantitative reverse transcription PCR (qRT-PCR) targeting ORF2 found four out of 49 (8%) lungs and one out of 48 (2%) enteric samples obtained from bovine diagnostic submissions positive for the novel bovine astrovirus (BAstV). In two strongly qRT-PCR-positive lung samples, intense novel BAstV nucleic acid signals were mainly localized in the cytoplasm of alveolar macrophages and mononuclear cells using RNAscope® in situ hybridization (ISH). Genetic analysis of two novel BAstV genomes determined from qRT-PCR positive samples found high similarity for ORF1ab nucleotide sequence (92.1% and 93.9%) to BAstV strain BSRI-1, while ORF2 nucleotide sequence was most similar to CAstV G2.1 (74.6% and 77.6%). Phylogenetic analysis of the novel BAstV sequences found a close genetic relationship to the single BAstV (BSRI-1) previously identified from a bovine respiratory sample as well as bovine and caprine AstVs identified from various tissues. Further research is needed to determine the clinical significance of BAstV in respiratory diseases.
Data from Reversal of Lactate and PD-1–mediated Macrophage Immunosuppression Controls Growth of PTEN/p53-deficient Prostate Cancer
Authors: Chaudagar Kiranj , Hieromnimon Hanna M. , Khurana Rimpi , Labadie Brian , Hirz Taghreed , Mei Shenglin , Hasan Raisa , Shafran Jordan , Kelley Anne , Apostolov Eva , Al-Eryani Ghamdan , Harvey Kate , Rameshbabu Srikrishnan , Loyd Mayme , Bynoe Kaela , Drovetsky Catherine , Solanki Ani , Markiewicz Erica , Zamora Marta , Fan Xiaobing , Schürer Stephan , Swarbrick Alex , Sykes David B. , Patnaik Akash
Published: April 2023

AbstractPurpose:
Phosphatase and tensin homolog (PTEN) loss of function occurs in approximately 50% of patients with metastatic castrate-resistant prostate cancer (mCRPC), and is associated with poor prognosis and responsiveness to standard-of-care therapies and immune checkpoint inhibitors. While PTEN loss of function hyperactivates PI3K signaling, combinatorial PI3K/AKT pathway and androgen deprivation therapy (ADT) has demonstrated limited anticancer efficacy in clinical trials. Here, we aimed to elucidate mechanism(s) of resistance to ADT/PI3K-AKT axis blockade, and to develop rational combinatorial strategies to effectively treat this molecular subset of mCRPC.
Experimental Design:
Prostate-specific PTEN/p53-deficient genetically engineered mice (GEM) with established 150–200 mm3 tumors, as assessed by ultrasound, were treated with either ADT (degarelix), PI3K inhibitor (copanlisib), or anti–PD-1 antibody (aPD-1), as single agents or their combinations, and tumors were monitored by MRI and harvested for immune, transcriptomic, and proteomic profiling, or ex vivo co-culture studies. Single-cell RNA sequencing on human mCRPC samples was performed using 10X Genomics platform.
Results:
Coclinical trials in PTEN/p53-deficient GEM revealed that recruitment of PD-1–expressing tumor-associated macrophages (TAM) thwarts ADT/PI3Ki combination–induced tumor control. The addition of aPD-1 to ADT/PI3Ki combination led to TAM-dependent approximately 3-fold increase in anticancer responses. Mechanistically, decreased lactate production from PI3Ki-treated tumor cells suppressed histone lactylation within TAM, resulting in their anticancer phagocytic activation, which was augmented by ADT/aPD-1 treatment and abrogated by feedback activation of Wnt/β-catenin pathway. Single-cell RNA-sequencing analysis in mCRPC patient biopsy samples revealed a direct correlation between high glycolytic activity and TAM phagocytosis suppression.
Conclusions:
Immunometabolic strategies that reverse lactate and PD-1–mediated TAM immunosuppression, in combination with ADT, warrant further investigation in patients with PTEN-deficient mCRPC.
Data from Targeting the Inducible T-cell Costimulator (ICOS) in Patients with Relapsed/Refractory T-follicular Helper Phenotype Peripheral T-cell and Angioimmunoblastic T-cell Lymphoma
Authors: Chavez Julio C. , Foss Francine M. , William Basem M. , Brammer Jonathan E. , Smith Sonali M. , Prica Anca , Zain Jasmine M. , Tuscano Joseph M. , Shah Harsh , Mehta-Shah Neha , Geethakumari Praveen Ramakrishnan , Wang Ben X. , Zantinge Stephanie , Wang Lisa , Zhang Ling , Boutrin Anmarie , Zhao Weiguang , Cheng Lily , Standifer Nathan , Hewitt Lisa , Enowtambong Enowmpey , Shao Weiping , Sharma Shringi , Carlesso Gianluca , Moscow Jeffrey A. , Siu Lillian L.
Published: April 2023

AbstractPurpose:
Proliferation of T-follicular helper (TFH) CD4+ T cells is a postulated pathogenic mechanism for T-cell non-Hodgkin lymphomas (T-NHL). The inducible T-cell costimulator (ICOS) is highly expressed by TFH, representing a potential target. MEDI-570 is a monoclonal antibody against ICOS, which eliminates ICOS+ cells in preclinical models.
Patients and Methods:
We report the safety, pharmacokinetics (PK), pharmacodynamics (PD), and clinical activity of MEDI-570 in T-NHL. NCI-9930 is a phase I, first-in-human study of MEDI-570 in relapsed/refractory malignant T-NHL known to express ICOS. MEDI-570 was administered intravenously every 3 weeks for up to 12 cycles. Primary endpoints were safety, dose-limiting toxicities (DLT), and recommended phase II dose (RP2D). Secondary and exploratory endpoints included efficacy parameters and various correlative studies. This study is supported by the National Cancer Institute (NCT02520791).
Results:
Twenty-three patients were enrolled and received MEDI-570 at five dose levels (0.01–3 mg/kg). Sixteen (70%) had angioimmunoblastic T-cell lymphoma (AITL); median age was 67 years (29–86) and the median prior lines of therapies was 3 (1–16). Most common grade 3 or 4 adverse events were decreased CD4+ T cells (57%), lymphopenia (22%), anemia (13%), and infusion-related reactions (9%). No DLTs were observed. The RP2D was determined at 3 mg/kg. Analysis of T-cell subsets showed reductions in CD4+ICOS+ T cells reflecting its effects on TFH cells. The response rate in AITL was 44%.
Conclusions:
MEDI-570 was well tolerated and showed promising clinical activity in refractory AITL. MEDI-570 resulted in sustained reduction of ICOS+ T lymphocytes.
Multicenter Phase I Trial of Ivosidenib as Maintenance Treatment Following Allogeneic Hematopoietic Cell Transplantation for IDH1-Mutated Acute Myeloid Leukemia
Authors: Fathi Amir T. , Kim Haesook T. , Soiffer Robert J. , Levis Mark J. , Li Shuli , Kim Annette S. , DeFilipp Zachariah , El-Jawahri Areej , McAfee Steve L. , Brunner Andrew M. , Amrein Philip C. , Mims Alice S. , Knight Laura W. , Kelley Devon , Bottoms AJ S. , Perry Lindsey H. , Wahl Jonathan L. , Brock Jennifer , Breton Elayne , Marchione Dylan M. , Ho Vincent T. , Chen Yi-Bin
Published: April 2023

Purpose:

Isocitrate dehydrogenase 1 (IDH1) mutations occur in 5% to 10% of patients with acute myeloid leukemia (AML). Ivosidenib is an IDH1 inhibitor, approved for use in patients with IDH1-mutated AML.

Patients and Methods:

We conducted a multicenter, phase I trial of maintenance ivosidenib following allogeneic hematopoietic cell transplantation (HCT) in patients with IDH1-mutated AML. Ivosidenib was initiated between days 30 and 90 following HCT and continued for up to 12 28-day cycles. The first dose level was 500 mg daily, with level reduction to 250 mg daily, if needed, in a 3 × 3 de-escalation design. Ten additional patients would then receive the MTD or recommended phase 2 dose (RP2D). The primary endpoint was establishing the MTD or RP2D of ivosidenib.

Results:

Eighteen patients were enrolled, of whom 16 initiated post-HCT ivosidenib. One dose-limiting toxicity, grade(g) 3 QTc prolongation, was observed. The RP2D was established at 500 mg daily. Attributable g≥3 adverse events were uncommon, with the most common being QTc prolongation in 2 patients. Eight patients discontinued maintenance, with only one due to adverse event. Six-month cumulative incidence (CI) of gII-IV aGVHD was 6.3%, and 2-year CI of all cGVHD was 63%. Two-year CI of relapse and nonrelapse mortality (NRM) were 19% and 0%, respectively. Two-year progression-free (PFS) was 81%, and 2-year overall survival (OS) was 88%.

Conclusions:

Ivosidenib is safe and well-tolerated as maintenance therapy following HCT. Cumulative incidence of relapse and NRM, as well as estimations of PFS and OS, were promising in this phase I study.
Supplementary Table 1, Figures 1 - 6 from The Novel Chk1 Inhibitor MK-8776 Sensitizes Human Leukemia Cells to HDAC Inhibitors by Targeting the Intra-S Checkpoint and DNA Replication and Repair
Authors: Dai Yun , Chen Shuang , Kmieciak Maciej , Zhou Liang , Lin Hui , Pei Xin-Yan , Grant Steven
Published: April 2023

PDF file - 1275K, Supplemental Table 1. Synergism between MK-8776 and SBHA or vorinostat in various human leukemia cell lines. Supplemental Figure S1. SBHA or vorinostat significantly potentiates lethality of MK-8776 in leukemia cell lines. Supplemental Figure S2. SBHA or vorinostat enhances Chk1 inhibition by MK-8776. Supplemental Figure S3. Disruption of Chk1 function increases S phase population and sensitizes cells to SBHA or vorinostat, in association with Cdt1 downregulation. Supplemental Figure S4. MK-8776/HDACIs abrogate intra-S-phase checkpoint and induce DNA damage in association with disruption of the CtIP/BRCA1 pathway that mediates DNA repair via HR. Supplemental Figure S5. Knock-down of p53 by shRNA sensitizes in p53-wt leukemia cells to the MK-8776/SBHA regimen, and this regimen is active against leukemia CD34+/CD38-/CD123+ primitive population. Supplemental Figure S6. The MK-8776/HDACI regimen is active against primary AML cells, while spare normal CD34+ cells.
Supplementary Tables from Transcriptomic and Protein Expression Analysis Reveals Clinicopathological Significance of Bloom Syndrome Helicase (BLM) in Breast Cancer
Authors: Arora Arvind , Abdel-Fatah Tarek M.A. , Agarwal Devika , Doherty Rachel , Moseley Paul M. , Aleskandarany Mohammed A. , Green Andrew R. , Ball Graham , Alshareeda Alaa T. , Rakha Emad A. , Chan Stephen Y.T. , Ellis Ian O. , Madhusudan Srinivasan
Published: April 2023

Supplementary Tables S1-S9: Supplementary Table S1: Clinicopathological characteristics in the METABRIC cohort Supplementary Table S2: External validation cohorts (pooled n = 2413). Supplementary Table S3: Clinicopathological characteristics of Nottingham cohort Supplementary Table S4: Antigens, primary antibodies, clone, source, optimal dilution and scoring system used for each immunohistochemical marker Supplementary Table S5: BLM (nuclear protein expression) in breast cancer Supplementary Table S6: BLM (cytoplasmic protein expression) in breast cancer Supplementary Table S7: BLM (nuclear and cytoplasmic protein co-expression) in breast cancer Supplementary Table S8. BLM - Rad51 nuclear co-expression and breast cancer Supplementary Table S9: Multivariate analysis in Nottingham cohort.
Supplementary Figure Legend from Single-Domain Antibody–Based and Linker-Free Bispecific Antibodies Targeting FcγRIII Induce Potent Antitumor Activity without Recruiting Regulatory T Cells
Authors: Rozan Caroline , Cornillon Amélie , Pétiard Corinne , Chartier Martine , Behar Ghislaine , Boix Charlotte , Kerfelec Brigitte , Robert Bruno , Pèlegrin André , Chames Patrick , Teillaud Jean-Luc , Baty Daniel
Published: April 2023

PDF file - 70K
Supplementary Table 1 from Contribution of ATM and ATR to the Resistance of Glioblastoma and Malignant Melanoma Cells to the Methylating Anticancer Drug Temozolomide
Authors: Eich Marcus , Roos Wynand Paul , Nikolova Teodora , Kaina Bernd
Published: April 2023

PDF file - 53 KB, Doubling times for selected cell lines
Supplementary Legends from Triptolide Induces the Expression of miR-142-3p: A Negative Regulator of Heat Shock Protein 70 and Pancreatic Cancer Cell Proliferation
Authors: MacKenzie Tiffany N. , Mujumdar Nameeta , Banerjee Sulagna , Sangwan Veena , Sarver Aaron , Vickers Selwyn , Subramanian Subbaya , Saluja Ashok K.
Published: April 2023

Supplementary Legends - PDF file 81K, Supplementary figure legends and supplementary table legends
Supplementary Figure 1 from GSK3 Inhibitors Regulate MYCN mRNA Levels and Reduce Neuroblastoma Cell Viability through Multiple Mechanisms, Including p53 and Wnt Signaling
Authors: Duffy David J. , Krstic Aleksandar , Schwarzl Thomas , Higgins Desmond G. , Kolch Walter
Published: April 2023

PDF - 122K, Figure S1. Additional expression regulation data. A. Comparison of mRNA-seq and RT-qPCR measurements for the same differentially expressed mRNAs in IMR32 cells upon 24h GSK3 inhibitor treatments. B. Time course of azakenpaullone mediated ectopic MYCN mRNA reduction in SY5Y-MYCN, measured by RT-qPCR. C. RT-qPCR results of GSK3 inhibitor regulation of c-MYC in CRC cell lines. D. pSMAD levels in IMR32 upon GSK3 inhibitor treatment. E. MDM2 mRNA expression level in response to GSK3 inhibition, mRNA-seq data. F. Wnt Agonist 1 and BIO treatment regulated the mRNA (top) and protein (bottom) of MYC genes in a similar manner.
Supplemental Table from Efficacy of Carboplatin Alone and in Combination with ABT888 in Intracranial Murine Models of BRCA-Mutated and BRCA–Wild-Type Triple-Negative Breast Cancer
Authors: Karginova Olga , Siegel Marni B. , Van Swearingen Amanda E.D. , Deal Allison M. , Adamo Barbara , Sambade Maria J. , Bazyar Soha , Nikolaishvili-Feinberg Nana , Bash Ryan , O'Neal Sara , Sandison Katie , Parker Joel S. , Santos Charlene , Darr David , Zamboni William , Lee Yueh Z. , Miller C. Ryan , Anders Carey K.
Published: April 2023

This file contains the differentially expressed genes and enriched pathways lists from the gene expression microarray analyses in Supplemental Table S1, with sample information in S1a. Lists are for the following comparisons: SUM149 Control vs. ABT888 (S1b), MDA-MB-436 Control vs. ABT888 (S1c), SUM149 Control vs. Carboplatin (S1d), MDA-MB-436 Control vs. Carboplatin (S1e), SUM149 Control vs Carboplatin+ABT888 (S1f), MDA-MB-436 Control vs Carboplatin+ABT888 (S1g), SUM149 Carboplatin vs Carboplatin+ABT888 (S1h), MDA-MB-436 Carboplatin vs Carboplatin+ABT888 (S1i). DAVID pathway enrichment analysis results are shown for the significantly up-regulated (S1j) and down-regulated (S1k) genes with Carboplatin treatment for both models.
Supplementary Figure 1 from Glioblastoma Cells Containing Mutations in the Cohesin Component STAG2 Are Sensitive to PARP Inhibition
Authors: Bailey Melanie L. , O'Neil Nigel J. , van Pel Derek M. , Solomon David A. , Waldman Todd , Hieter Philip
Published: April 2023

PDF file - 168K, Supp. Fig. 1- A) Western blot of levels of cohesin components in H4 and H4 STAG2 KI cells in inputs and after RAD21 immunoprecipitation. B-C) Western blots of poly(ADP-ribose) (PAR) and STAG2 levels in B) H4 and C) 42MGBA cells with and without PARP inhibitor treatment. D) Western blot of core cohesin components SMC1, SMC3 and RAD21 in untreated and olaparib-treated lysates from H4 cell lines. E) Levels of PARP cleavage in 42MGBA cells with and without olaparib. Untreated cells exhibit extensive PARP auto-PARylation (bracket) which is inhibited after treatment with olaparib.
Supplementary Table 1 from Hedgehog Pathway Inhibition in Chondrosarcoma Using the Smoothened Inhibitor IPI-926 Directly Inhibits Sarcoma Cell Growth
Authors: Campbell Veronica T. , Nadesan Puviindran , Ali S. Amanda , Wang Chang Ye Yale , Whetstone Heather , Poon Raymond , Wei Qingxia , Keilty John , Proctor Jennifer , Wang Lauren W. , Apte Suneel S. , McGovern Karen , Alman Benjamin A. , Wunder Jay S.
Published: April 2023

PDF - 71K, A list of the solid tumor xenografts tested for regulation of human or murine Gli1 following IPI-926 treatment, as measured by q-RT-PCR on whole tumor RNA extracts.
Supplementary Figure 1 from Regulation of SRC Kinases by microRNA-3607 Located in a Frequently Deleted Locus in Prostate Cancer
Authors: Saini Sharanjot , Majid Shahana , Shahryari Varahram , Tabatabai Z. Laura , Arora Sumit , Yamamura Soichiro , Tanaka Yuichiro , Dahiya Rajvir , Deng Guoren
Published: April 2023

PDF - 176K, Relative miR-3607 expression levels in PC3/Du145/LNCaP cells in mock/miR-CON/ miR-3607 transfected cells as assessed by quantitative RT-PCR analysis.
Supplementary Movie 4 from Tivantinib (ARQ 197) Exhibits Antitumor Activity by Directly Interacting with Tubulin and Overcomes ABC Transporter–Mediated Drug Resistance
Authors: Aoyama Aki , Katayama Ryohei , Oh-hara Tomoko , Sato Shigeo , Okuno Yasushi , Fujita Naoya
Published: April 2023

Control (SK-MEL-28 Fucci).
Supplementary Figure 4 from Antitumor Activity of KW-2450 against Triple-Negative Breast Cancer by Inhibiting Aurora A and B Kinases
Authors: Kai Kazuharu , Kondo Kimie , Wang Xiaoping , Xie Xuemei , Pitner Mary K. , Reyes Monica E. , Torres-Adorno Angie M. , Masuda Hiroko , Hortobagyi Gabriel N. , Bartholomeusz Chandra , Saya Hideyuki , Tripathy Debu , Sen Subrata , Ueno Naoto T.
Published: April 2023

Knockdown of Mad2 or BubR1 in TNBC cells treated with KW-2450.
Supplementary Figure 2 from Multivalent Scaffold Proteins as Superagonists of TRAIL Receptor 2–Induced Apoptosis
Authors: Swers Jeffery S. , Grinberg Luba , Wang Lin , Feng Hui , Lekstrom Kristen , Carrasco Rosa , Xiao Zhan , Inigo Ivan , Leow Ching Ching , Wu Herren , Tice David A. , Baca Manuel
Published: April 2023

PDF file - 85K, Supplementary Figure S2: Specificity of TRAILR2-induced cell killing. TRAIL-sensitive cell lines were incubated with 100pM G6T8 and different concentrations of soluble TRAILR2-Fc fusion protein.
Supplementary Figure 1 from The HSP90 Inhibitor NVP-AUY922 Potently Inhibits Non–Small Cell Lung Cancer Growth
Authors: Garon Edward B. , Finn Richard S. , Hamidi Habib , Dering Judy , Pitts Sharon , Kamranpour Naeimeh , Desai Amrita J. , Hosmer Wylie , Ide Susan , Avsar Emin , Jensen Michael Rugaard , Quadt Cornelia , Liu Manway , Dubinett Steven M. , Slamon Dennis J.
Published: April 2023

PDF - 769K, Heat maps from microarray analyses of gene expression changes seen in at least 13 of 16 experiments after exposure to NVP-AUY922
Supplemental Figure 2 from Clinical Utility of a Blood-Based BRAFV600E Mutation Assay in Melanoma
Authors: Panka David J. , Buchbinder Elizabeth , Giobbie-Hurder Anita , Schalck Aislyn P. , Montaser-Kouhsari Laleh , Sepehr Alireza , Lawrence Donald P. , McDermott David F. , Cohen Rachel , Carlson Alexander , Wargo Jennifer A. , Merritt Ryan , Seery Virginia J. , Hodi F. Stephen , Gunturi Anasuya , Fredrick Dennie , Atkins Michael B. , Iafrate A. John , Flaherty Keith T. , Mier James W. , Sullivan Ryan J.
Published: April 2023

Serial BRAF V600E levels (pg) compared to tumor volume by RECIST from 11 patients treated with vemurafenib and 6 patients treated with dabrafenib and trametinib until removed from the study due to progressive disease (the time of disease progression is shown with an arrow).
Supplementary Figure 2 from Overexpression of DDX43 Mediates MEK Inhibitor Resistance through RAS Upregulation in Uveal Melanoma Cells
Authors: Ambrosini Grazia , Khanin Raya , Carvajal Richard D. , Schwartz Gary K.
Published: April 2023

PDF file - 365K, The MEK inhibitor-resistant cells re-express pERK at later time point after treatment.
Supplementary Figure 2 from NF-κB Inhibition by Bortezomib Permits IFN-γ–Activated RIP1 Kinase–Dependent Necrosis in Renal Cell Carcinoma
Authors: Thapa Roshan J. , Chen Peirong , Cheung Mitchell , Nogusa Shoko , Pei Jianming , Peri Suraj , Testa Joseph R. , Balachandran Siddharth
Published: April 2023

PDF file - 425K, Supplementary Figure 2. Blockade of TNF-α-induced NF-κB signaling by bortezomib and IKKβ inhibitor.
Supplementary Figure 6 from Antitumor Activity of the Glutaminase Inhibitor CB-839 in Triple-Negative Breast Cancer
Authors: Gross Matt I. , Demo Susan D. , Dennison Jennifer B. , Chen Lijing , Chernov-Rogan Tania , Goyal Bindu , Janes Julie R. , Laidig Guy J. , Lewis Evan R. , Li Jim , MacKinnon Andrew L. , Parlati Francesco , Rodriguez Mirna L.M. , Shwonek Peter J. , Sjogren Eric B. , Stanton Timothy F. , Wang Taotao , Yang Jinfu , Zhao Frances , Bennett Mark K.
Published: April 2023

PDF - 100K, Supplementary Figure S6. The basal glutamate to glutamine ratio and the fold change in glutamine and glutamate following CB-839 treatment is greater in TNBC cells than receptor-positive cell lines.
Supplementary Figure 1 from NF-κB Inhibition by Bortezomib Permits IFN-γ–Activated RIP1 Kinase–Dependent Necrosis in Renal Cell Carcinoma
Authors: Thapa Roshan J. , Chen Peirong , Cheung Mitchell , Nogusa Shoko , Pei Jianming , Peri Suraj , Testa Joseph R. , Balachandran Siddharth
Published: April 2023

PDF file - 5653K, Supplementary Figure 1. Genomic copy number analysis of HRC cell lines.
Supplementary Table 1 from Polo-like Kinase Inhibitor Ro5203280 Has Potent Antitumor Activity in Nasopharyngeal Carcinoma
Authors: Cheung Arthur Kwok Leung , Yan Ip Joseph Chok , Lung Hong Lok , Wu Jim Zhen , Tsao Sai Wah , Lung Maria Li
Published: April 2023

PDF file - 31K, Kinases not inhibited by Ro5203280.
Supplementary Figure 1 from Antitumor Activity of the Glutaminase Inhibitor CB-839 in Triple-Negative Breast Cancer
Authors: Gross Matt I. , Demo Susan D. , Dennison Jennifer B. , Chen Lijing , Chernov-Rogan Tania , Goyal Bindu , Janes Julie R. , Laidig Guy J. , Lewis Evan R. , Li Jim , MacKinnon Andrew L. , Parlati Francesco , Rodriguez Mirna L.M. , Shwonek Peter J. , Sjogren Eric B. , Stanton Timothy F. , Wang Taotao , Yang Jinfu , Zhao Frances , Bennett Mark K.
Published: April 2023

PDf - 136K, Supplementary Figure S1. CB-839 is a non-competitive inhibitor of rHu-GAC.
Supplementary Figure 4 from Polo-like Kinase Inhibitor Ro5203280 Has Potent Antitumor Activity in Nasopharyngeal Carcinoma
Authors: Cheung Arthur Kwok Leung , Yan Ip Joseph Chok , Lung Hong Lok , Wu Jim Zhen , Tsao Sai Wah , Lung Maria Li
Published: April 2023

PDF file - 539K, Western blot analysis of PLK, cell cycle control, and DNA damage-related signaling pathways analysis of tumors in different Ro5203280 dosage treatment groups of HONE1 and C666-1.
Supplementary Figure Legends from XPO1 (CRM1) Inhibition Represses STAT3 Activation to Drive a Survivin-Dependent Oncogenic Switch in Triple-Negative Breast Cancer
Authors: Cheng Yan , Holloway Michael P. , Nguyen Kevin , McCauley Dilara , Landesman Yosef , Kauffman Michael G. , Shacham Sharon , Altura Rachel A.
Published: April 2023

PDF file - 84K
Supplementary Figure 3 from Polo-like Kinase Inhibitor Ro5203280 Has Potent Antitumor Activity in Nasopharyngeal Carcinoma
Authors: Cheung Arthur Kwok Leung , Yan Ip Joseph Chok , Lung Hong Lok , Wu Jim Zhen , Tsao Sai Wah , Lung Maria Li
Published: April 2023

PDF file - 71K, DAPI staining of cells with mitotic abnormalities after 24 hours treatment with 200 nM Ro5203280 of NPC and NP cell lines.
Supplementary Figures 1 - 4 from The AMPK Inhibitor Compound C Is a Potent AMPK-Independent Antiglioma Agent
Authors: Liu Xiaona , Chhipa Rishi Raj , Nakano Ichiro , Dasgupta Biplab
Published: April 2023

PDF file - 589K, Figure S1. Antiproliferative effects of Compound C in glioma cells. Figure S2. Compound C induces AMPK-independent autophagy and the antiproliferative effects of Compound C are not blocked by autophagy and apoptosis inhibitors. Figure S3. Constitutively active Akt fails to block Compound C?s antiproliferative action. Figure S4. Effects of Compound C on glioma cell cycle.
Supplementary Figure Legend from RAD51C-Deficient Cancer Cells Are Highly Sensitive to the PARP Inhibitor Olaparib
Authors: Min Ahrum , Im Seock-Ah , Yoon Young-Kwang , Song Sang-Hyun , Nam Hyun-Jin , Hur Hyung-Seok , Kim Hwang-Phill , Lee Kyung-Hun , Han Sae-Won , Oh Do-Youn , Kim Tae-You , O'Connor Mark J. , Kim Woo-Ho , Bang Yung-Jue
Published: April 2023

PDF file - 176K
Supplementary Figure S7 from Preclinical Evaluation of Transcriptional Targeting Strategy for Human Hepatocellular Carcinoma in an Orthotopic Xenograft Mouse Model
Authors: Sia Kian Chuan , Huynh Hung , Chung Alexander Yaw Fui , Ooi London Lucien Peng Jin , Lim Kiat Hon , Hui Kam Man , Lam Paula Yeng Po
Published: April 2023

Supplementary Figure S7 - PDF file 643K, Chemical structure of DZNep (3-Deazaneplanocin A). Adapted from Sigma-Aldrich (catalog number SML0305)
Supplementary Figure 2 from Tirapazamine Sensitizes Hepatocellular Carcinoma Cells to Topoisomerase I Inhibitors via Cooperative Modulation of Hypoxia-Inducible Factor-1α
Authors: Cai Tian-Yu , Liu Xiao-Wen , Zhu Hong , Cao Ji , Zhang Jun , Ding Ling , Lou Jian-Shu , He Qiao-Jun , Yang Bo
Published: April 2023

PDF file - 166K, Figure S2. TPZ enhanced the cytotoxicity elicited by several topoisomerase I inhibitors in Bel-7402 cells under hypoxia.