bluuebunny/crossref_metadata_2025 huggingface dataset:
Using Communication Theory for Health Promotion
Authors: Edgar Timothy , Volkman Julie E.
Published: August 2012

According to the U.S. Department of Health and Human Services, health communication is “the study and use of communication strategies to inform and influence individual and community decisions that enhance health.” The purpose of this article is to look at how health educators can use communication theory to create messages that are innovative, relatable, and motivating to intended audiences. Three specific communication theories are presented, along with examples of how they have been successfully used in behavior change initiatives. These three theories are offered in an effort to stimulate further investigation into how theory supports the creation of targeted, tailored, and effective communication strategies.
The Liminal Hero
Authors: Poulos Christopher N.
Published: August 2012

This critical autoethnography is about my encounters, throughout my life, with the “Western” (liminal) heroes and villains, who came to life on screens large and small. It is about the shifting visions of heroism, villainy, and violence in our culture. It is about walking the borderlands between all these territories. And it is about my own gradual awakening.
Re-Searching My Scar
Authors: Schwartzman Roy
Published: August 2012

This essay juxtaposes the process of “Othering” in the 1956 John Ford western The Searchers with my own indoctrination into White privilege as a child growing up in suburban Atlanta during the mid-to-late 1960s. The film’s stark portrayal of anti-Native American attitudes confronts the problematic construction of the non-White racial “Other” in westerns as a threat to racial and sexual purity. Its relentless pursuit of the implications of racism triggers my own confrontation with the subtle but persistent degradations of African Americans in my upbringing.
Assessment and Treatment of Feeding-Related Problem Behaviors of a 16-Year-Old Girl With PDD-NOS
Authors: Knapp Vicki Madaus , Simmons Lori , Verstraete Steven K. , McAdam David B.
Published: August 2012

This case study describes the successful implementation of a behavioral intervention for the mealtime problem behaviors of a 16-year-old girl with Pervasive Developmental Disorder Not Otherwise Specified attending a private school for students with developmental disabilities. Initially, an analogue-functional analysis using a pairwise single-case experimental design was conducted, and based on the results of this assessment, a multicomponent function-based intervention was developed. The results obtained demonstrated that interventions for problem behaviors developed in hospital-based clinics can be implemented in community-based educational settings successfully. The results also demonstrated that behavioral intervention strategies shown to be effective with young children with developmental disabilities also work for a teenager with an autism spectrum disorder, illustrating the generality of a behavioral approach to feeding difficulties. Methodological limitations of the case study, advantages of a community-based treatment approach, and recommendations for clinicians are provided.
The Case of Rose
Authors: Lehman Erica E.
Published: August 2012

As the percentage of adults aged 60 and above in the United States grows, the need for clinicians who can work effectively with older adults becomes increasingly vital. In this article, the case of a 77-year-old Caucasian woman diagnosed with schizoaffective disorder and anorexia nervosa is presented. First, the author reviews research and treatments used with schizoaffective disorder and anorexia nervosa in older adults. A theoretical formulation is made using Bob Knight’s contextual, cohort-based, maturity, specific-challenge model as well as a cognitive-behavioral model. The article reviews the first 6 months of treatment with the client and presents a brief discussion of the challenges experienced while working as part of an interdisciplinary team.
Biomechanical effect of isolated capitate shortening in Kienböck’s disease: an anatomical study
Authors: Werber K.-D. , Schmelz R. , Peimer C. A. , Wagenpfeil S. , Machens H.-G. , Lohmeyer J. A.
Published: August 2012

Multiple operations have been proposed to slow the progression of osteonecrosis and secondary carpal damage in Kienböck’s disease. To assess the biomechanical changes after capitate shorting, we inserted pressure-testing devices into the carpal and radiocarpal joints in an anatomical study. Pressure sensors were placed into eight thawed non-fixated human cadaver arms to measure the forces transmitted in physiological loading. Longitudinal 9.8 N and 19.6 N forces were applied before and after capitate shortening. After capitate shortening, significant load reduction on the lunate was evident in all specimens. An average decrease of 49% was seen under a 9.8 N load and 56% under a 19.6 N load. The load was transferred to the radial and ulnar intercarpal joints. More relief of pressure on the lunate after isolated capitate shortening is achieved with a shallow angle between the scaphoid and capitate in the posteroanterior radiograph.
The Masculine Image of Presidents As Sporting Figures
Authors: Moore Aaron J. , Dewberry David
Published: August 2012

This article explores the role of the president identifying himself as a sporting figure, which can be successful as well as detrimental in the presidents’ overall public relations strategy, through an ideological criticism lens. To make this argument, the authors start by examining the role of sports in American popular culture and its relationship to a strong masculine image. Recognizing the value of publicity in the mediated political world, they then trace the evolution of the president from Eisenhower to Obama identifying himself as a sporting figure through the mass media. The article specifically focuses on the two most recent presidents’ use of sports and its effect on their masculine image. The authors conclude by addressing the implications of a future female president’s strategic use of sports.
Consuming My Way Gay
Authors: Eichler Matthew
Published: August 2012

As members of consumerist societies, we are socialized into what it means to be good citizens and participate in society through our consumption. For many, this is taught in the home, yet for lesbian, gay, bisexual, transgender, and queer (LGBTQ) individuals, home is often not a source of reliable information about our identities. As such, LGBTQ individuals turn to the marketplace to seek information about their sexual and gender identities. This autoethnographic account shares, through three vignettes, how coming out as a queer man is shaped by consumptive pedagogy—that is, learning through consumption. First, material goods are explored as the signifier of sexual orientation. Then, the gay bar as marketplace and the online marketplace for relationships are explored.
Mammalian target of rapamycin regulates neutrophil extracellular trap formation via induction of hypoxia-inducible factor 1 α
Authors: McInturff Alison M. , Cody Mark J. , Elliott Elizabeth A. , Glenn Jared W. , Rowley Jesse W. , Rondina Matthew T. , Yost Christian C.
Published: August 2012

Neutrophils are highly specialized innate immune effector cells that evolved for antimicrobial host defense. In response to inflammatory stimuli and pathogens, they form neutrophil extracellular traps (NETs), which capture and kill extracellular microbes. Deficient NET formation predisposes humans to severe infection, but, paradoxically, dysregulated NET formation contributes to inflammatory vascular injury and tissue damage. The molecular pathways and signaling mechanisms that control NET formation remain largely uncharacterized. Using primary human neutrophils and genetically manipulated myeloid leukocytes differentiated to surrogate neutrophils, we found that mammalian target of rapamycin (mTOR) regulates NET formation by posttranscriptional control of expression of hypoxia-inducible factor 1 α (HIF-1α), a critical modulator of antimicrobial defenses. Next-generation RNA sequencing, assays of mRNA and protein expression, and analysis of NET deployment by live cell imaging and quantitative histone release showed that mTOR controls NET formation and translation of HIF-1α mRNA in response to lipopolysaccharide. Pharmacologic and genetic knockdown of HIF-1α expression and activity inhibited NET deployment, and inhibition of mTOR and HIF-1α inhibited NET-mediated extracellular bacterial killing. Our studies define a pathway to NET formation involving 2 master regulators of immune cell function and identify potential points of molecular intervention in strategies to modify NETs in disease.
Endothelial cells provide an instructive niche for the differentiation and functional polarization of M2-like macrophages
Authors: He Huanhuan , Xu Jingying , Warren Carmen M. , Duan Dan , Li Xinmin , Wu Lily , Iruela-Arispe M. Luisa
Published: August 2012

Endothelial cells and macrophages are known to engage in tight and specific interactions that contribute to the modulation of vascular function. Here we show that adult endothelial cells provide critical signals for the selective growth and differentiation of macrophages from several hematopoietic progenitors. The process features the formation of well-organized colonies that exhibit progressive differentiation from the center to the periphery and toward an M2-like phenotype, characterized by enhanced expression of Tie2 and CD206/Mrc1. These colonies are long-lived depending on the contact with the endothelium; removal of the endothelial monolayer results in rapid colony dissolution. We further found that Csf1 produced by the endothelium is critical for the expansion of the macrophage colonies and that blockade of Csf1 receptor impairs colony growth. Functional analyses indicate that these macrophages are capable of accelerating angiogenesis, promoting tumor growth, and effectively engaging in tight associations with endothelial cells in vivo. These findings uncover a critical role of endothelial cells in the induction of macrophage differentiation and their ability to promote further polarization toward a proangiogenic phenotype. This work also highlights some of the molecules underlying the M2-like differentiation, a process that is relevant to the progression of both developmental and pathologic angiogenesis.
SMILE for natural killer/T-cell lymphoma: analysis of safety and efficacy from the Asia Lymphoma Study Group
Authors: Kwong Yok-Lam , Kim Won Seog , Lim Soon Thye , Kim Seok Jin , Tang Tiffany , Tse Eric , Leung Anskar Y. H. , Chim Chor-Sang
Published: August 2012

Natural killer/T-cell lymphoma is rare and aggressive, with poor outcome. Optimal treatment remains unclear. A novel regimen dexamethasone, methotrexate, ifosfamide, l-asparaginase, and etoposide (SMILE) showed promise in phase 1/2 studies with restrictive recruitment criteria. To define the general applicability of SMILE, 43 newly diagnosed and 44 relapsed/refractory patients (nasal, N = 60, nonnasal, N = 21; disseminated, N = 6; male, N = 59; female, N = 28) at a median age of 51 years (23-83 years) were treated. Poor-risk factors included stage III/IV disease (56%), international prognostic index of 3 to 5 (43%), and Korean prognostic scores of 3 to 4 (41%). A median of 3 (0-6; total = 315) courses of SMILE were administered. Significant toxicities included grade 3/4 neutropenia (N = 57; 5 sepsis-related deaths); grade 3/4 thrombocytopenia (N = 36); and nephrotoxicity (N = 15; 1 acute renal failure and death). Interim analysis after 2 to 3 cycles showed complete remission rate of 56%, partial remission rate of 22%, giving an overall response rate of 78%. On treatment completion, the overall-response rate became 81% (complete remission = 66%, partial remission = 15%). Response rates were similar for newly diagnosed or relapsed/refractory patients. At a median follow-up of 31 months (1-84 months), the 5-year overall survival was 50% and 4-year disease-free-survival was 64%. Multivariate analysis showed that international prognostic index was the most significant factor impacting on outcome and survivals.
An effective method for network module extraction from microarray data
Authors: Mahanta Priyakshi , Ahmed Hasin A , Bhattacharyya Dhruba K , Kalita Jugal K
Published: August 2012

Background

The development of high-throughput Microarray technologies has provided various opportunities to systematically characterize diverse types of computational biological networks. Co-expression network have become popular in the analysis of microarray data, such as for detecting functional gene modules.

Results

This paper presents a method to build a co-expression network (CEN) and to detect network modules from the built network. We use an effective gene expression similarity measure called NMRS (Normalized mean residue similarity) to construct the CEN. We have tested our method on five publicly available benchmark microarray datasets. The network modules extracted by our algorithm have been biologically validated in terms of Q value and p value.

Conclusions

Our results show that the technique is capable of detecting biologically significant network modules from the co-expression network. Biologist can use this technique to find groups of genes with similar functionality based on their expression information.
Giant viruses coexisted with the cellular ancestors and represent a distinct supergroup along with superkingdoms Archaea, Bacteria and Eukarya
Authors: Nasir Arshan , Kim Kyung Mo , Caetano-Anolles Gustavo
Published: August 2012

Background

The discovery of giant viruses with genome and physical size comparable to cellular organisms, remnants of protein translation machinery and virus-specific parasites (virophages) have raised intriguing questions about their origin. Evidence advocates for their inclusion into global phylogenomic studies and their consideration as a distinct and ancient form of life.

Results

Here we reconstruct phylogenies describing the evolution of proteomes and protein domain structures of cellular organisms and double-stranded DNA viruses with medium-to-very-large proteomes (giant viruses). Trees of proteomes define viruses as a ‘fourth supergroup’ along with superkingdoms Archaea, Bacteria, and Eukarya. Trees of domains indicate they have evolved via massive and primordial reductive evolutionary processes. The distribution of domain structures suggests giant viruses harbor a significant number of protein domains including those with no cellular representation. The genomic and structural diversity embedded in the viral proteomes is comparable to the cellular proteomes of organisms with parasitic lifestyles. Since viral domains are widespread among cellular species, we propose that viruses mediate gene transfer between cells and crucially enhance biodiversity.

Conclusions

Results call for a change in the way viruses are perceived. They likely represent a distinct form of life that either predated or coexisted with the last universal common ancestor (LUCA) and constitute a very crucial part of our planet’s biosphere.
Developing the anemone Aiptasia as a tractable model for cnidarian-dinoflagellate symbiosis: the transcriptome of aposymbiotic A. pallida
Authors: Lehnert Erik M , Burriesci Matthew S , Pringle John R
Published: August 2012

Background

Coral reefs are hotspots of oceanic biodiversity, forming the foundation of ecosystems that are important both ecologically and for their direct practical impacts on humans. Corals are declining globally due to a number of stressors, including rising sea-surface temperatures and pollution; such stresses can lead to a breakdown of the essential symbiotic relationship between the coral host and its endosymbiotic dinoflagellates, a process known as coral bleaching. Although the environmental stresses causing this breakdown are largely known, the cellular mechanisms of symbiosis establishment, maintenance, and breakdown are still largely obscure. Investigating the symbiosis using an experimentally tractable model organism, such as the small sea anemone Aiptasia, should improve our understanding of exactly how the environmental stressors affect coral survival and growth.

Results

We assembled the transcriptome of a clonal population of adult, aposymbiotic (dinoflagellate-free) Aiptasia pallida from ~208 million reads, yielding 58,018 contigs. We demonstrated that many of these contigs represent full-length or near-full-length transcripts that encode proteins similar to those from a diverse array of pathways in other organisms, including various metabolic enzymes, cytoskeletal proteins, and neuropeptide precursors. The contigs were annotated by sequence similarity, assigned GO terms, and scanned for conserved protein domains. We analyzed the frequency and types of single-nucleotide variants and estimated the size of the Aiptasia genome to be ~421 Mb. The contigs and annotations are available through NCBI (Transcription Shotgun Assembly database, accession numbers JV077153-JV134524) and at http://pringlelab.stanford.edu/projects.html.

Conclusions

The availability of an extensive transcriptome assembly for A. pallida will facilitate analyses of gene-expression changes, identification of proteins of interest, and other studies in this important emerging model system.
Genetic diversity within the genus Francisella as revealed by comparative analyses of the genomes of two North American isolates from environmental sources
Authors: Siddaramappa Shivakumara , Challacombe Jean F , Petersen Jeannine M , Pillai Segaran , Kuske Cheryl R
Published: August 2012

Background

Francisella tularensis is an intracellular pathogen that causes tularemia in humans and the public health importance of this bacterium has been well documented in recent history. Francisella philomiragia, a distant relative of F. tularensis, is thought to constitute an environmental lineage along with Francisella novicida. Nevertheless, both F. philomiragia and F. novicida have been associated with human disease, primarily in immune-compromised individuals. To understand the genetic relationships and evolutionary contexts among different lineages within the genus Francisella, the genome of Francisella spp. strain TX07-7308 was sequenced and compared to the genomes of F. philomiragia strains ATCC 25017 and 25015, F. novicida strain U112, and F. tularensis strain Schu S4.

Results

The size of strain ATCC 25017 chromosome was 2,045,775 bp and contained 1,983 protein-coding genes. The size of strain TX07-7308 chromosome was 2,035,931 bp and contained 1,980 protein-coding genes. Pairwise BLAST comparisons indicated that strains TX07-7308 and ATCC 25017 contained 1,700 protein coding genes in common. NUCmer analyses revealed that the chromosomes of strains TX07-7308 and ATCC 25017 were mostly collinear except for a few gaps, translocations, and/or inversions. Using the genome sequence data and comparative analyses with other members of the genus Francisella (e.g., F. novicida strain U112 and F. tularensis strain Schu S4), several strain-specific genes were identified. Strains TX07-7308 and ATCC 25017 contained an operon with six open reading frames encoding proteins related to enzymes involved in thiamine biosynthesis that was absent in F. novicida strain U112 and F. tularensis strain Schu S4. Strain ATCC 25017 contained an operon putatively involved in lactose metabolism that was absent in strain TX07-7308, F. novicida strain U112, and F. tularensis strain Schu S4. In contrast, strain TX07-7308 contained an operon putatively involved in glucuronate metabolism that was absent in the genomes of strain ATCC 25017, F. novicida strain U112, and F. tularensis strain Schu S4. The polymorphic nature of polysaccharide biosynthesis/modification gene clusters among different Francisella strains was also evident from genome analyses.

Conclusions

From genome comparisons, it appeared that genes encoding novel functions have contributed to the metabolic enrichment of the environmental lineages within the genus Francisella. The inability to acquire new genes coupled with the loss of ancestral traits and the consequent reductive evolution may be a cause for, as well as an effect of, niche selection of F. tularensis. Sequencing and comparison of the genomes of more isolates are required to obtain further insights into the ecology and evolution of different species within the genus Francisella.
High yield expression of catalytically active USP18 (UBP43) using a Trigger Factor fusion system
Authors: Basters Anja , Ketscher Lars , Deuerling Elke , Arkona Christoph , Rademann Jörg , Knobeloch Klaus-Peter , Fritz Günter
Published: August 2012

Background

Covalent linkage of the ubiquitin-like protein ISG15 interferes with viral infection and USP18 is the major protease which specifically removes ISG15 from target proteins. Thus, boosting ISG15 modification by protease inhibition of USP18 might represent a new strategy to interfere with viral replication. However, so far no heterologous expression system was available to yield sufficient amounts of catalytically active protein for high-throughput based inhibitor screens.

Results

High-level heterologous expression of USP18 was achieved by applying a chaperone-based fusion system in E. coli. Pure protein was obtained in a single-step on IMAC via a His6-tag. The USP18 fusion protein exhibited enzymatic activity towards cell derived ISG15 conjugated substrates and efficiently hydrolyzed ISG15-AMC. Specificity towards ISG15 was shown by covalent adduct formation with ISG15 vinyl sulfone but not with ubiquitin vinyl sulfone.

Conclusion

The results presented here show that a chaperone fusion system can provide high yields of proteins that are difficult to express. The USP18 protein obtained here is suited to setup high-throughput small molecule inhibitor screens and forms the basis for detailed biochemical and structural characterization.
A Chinese herbal formula "Gan-Lu-Yin" suppresses vascular smooth muscle cell migration by inhibiting matrix metalloproteinase-2/9 through the PI3K/AKT and ERK signaling pathways
Authors: Chien Yi-Chung , Sheu Ming-Jyh , Wu Chieh-Hsi , Lin Wen-Hsin , Chen Ying-Yi , Cheng Po-Liang , Cheng Hsu-Chen
Published: August 2012

Background

This study was to explore the effects of Gan-Lu-Yin (GLY) on the migration of vascular smooth muscle cells (VSMCs) induced by fetal bovine serum and on neointima formation in a rat model of carotid artery balloon injury.

Methods

VSMCs were treated with different concentrations of GLY, and then analyzed with Flow cytometric analysis, zymography, transwell, and western blotting. SD rats received balloon-injury were analyzed with H&E staining.

Results

Our results showed that GLY significantly decreased the thickness of neointima. The inhibition by non-cytoxic doses of GLY of VSMCs migration was through its negative regulatory effects on phosphorylated ERK1/2, PI3K/AKT, and FAK. The data showed that GLY can inhibit the migration of VSMCs cells, and might block injury-induced neointima hyperplasia via the inhibition of VSMCs migration, without inducing apoptosis.

Conclusions

These observations provide a mechanism of GLY in attenuating cell migration, thus as a potential intervention for restenosis.
Performance of “VIKIA Malaria Ag Pf/Pan” (IMACCESS®), a new malaria rapid diagnostic test for detection of symptomatic malaria infections
Authors: Chou Monidarin , Kim Saorin , Khim Nimol , Chy Sophy , Sum Sarorn , Dourng Dany , Canier Lydie , Nguon Chea , Ménard Didier
Published: August 2012

Background

Recently, IMACCESS® developed a new malaria test (VIKIA Malaria Ag Pf/Pan™), based on the detection of falciparum malaria (HRP-2) and non-falciparum malaria (aldolase).

Methods

The performance of this new malaria rapid diagnostic test (RDT) was assessed using 1,000 febrile patients seeking malaria treatment in four health centres in Cambodia from August to December 2011. The results of the VIKIA Malaria Ag Pf/Pan were compared with those obtained by microscopy, the CareStart Malaria™ RDT (AccessBio®) which is currently used in Cambodia, and real-time PCR (as “gold standard”).

Results

The best performances of the VIKIA Malaria Ag Pf/Pan™ test for detection of both Plasmodium falciparum and non-P. falciparum were with 20–30 min reading times (sensitivity of 93.4% for P. falciparum and 82.8% for non-P. falciparum and specificity of 98.6% for P. falciparum and 98.9% for non-P. falciparum) and were similar to those for the CareStart Malaria™ test.

Conclusions

This new RDT performs similarly well as other commercially available tests (especially the CareStart Malaria™ test, used as comparator), and conforms to the World Health Organization’s recommendations for RDT performance. It is a good alternative tool for the diagnosis of malaria in endemic areas.
Rab31 expression levels modulate tumor-relevant characteristics of breast cancer cells
Authors: Grismayer Bettina , Sölch Susanne , Seubert Bastian , Kirchner Thomas , Schäfer Sonja , Baretton Gustavo , Schmitt Manfred , Luther Thomas , Krüger Achim , Kotzsch Matthias , Magdolen Viktor
Published: August 2012

Background

Rab proteins constitute a large family of monomeric GTP-binding proteins that regulate intracellular vesicle transport. Several Rab proteins, including rab31, have been shown to affect cancer progression and are related with prognosis in various types of cancer including breast cancer. Recently, the gene encoding rab31 was found to be overexpressed in estrogen receptor-positive breast cancer tissue. In a previous study we found a significant association of high rab31 mRNA expression with poor prognosis in node-negative breast cancer patients. In the present study, we aimed to investigate the impact of rab31 (over)-expression on important aspects of tumor progressionin vitroandin vivo.

Methods

Breast cancer cells displaying low (MDA-MB-231) or no (CAMA-1) endogenous rab31 expression were stably transfected with a rab31 expression plasmid. Batch-transfected cells as well as selected cell clones, expressing different levels of rab31 protein, were analyzed with regard to proliferation, cell adhesion, the invasive capacity of tumor cells, andin vivoin a xenograft tumor model. Polyclonal antibodies directed to recombinantly expressed rab31 were generated and protein expression analyzed by immunohistochemistry, Western blot analysis, and a newly developed sensitive ELISA.

Results

Elevated rab31 protein levels were associated with enhanced proliferation of breast cancer cells. Interestingly, weak to moderate overexpression of rab31 in cell lines with no detectable endogenous rab31 expression was already sufficient to elicit distinct effects on cell proliferation. By contrast, increased expression of rab31 in breast cancer cells led to reduced adhesion towards several extracellular matrix proteins and decreased invasive capacity through MatrigelTM. Again, the rab31-mediated effects on cell adhesion and invasion were dose-dependent. Finally, in a xenograft mouse model, we observed a significantly impaired metastatic dissemination of rab31 overexpressing MDA-MB-231 breast cancer cells to the lung.

Conclusions

Overexpression of rab31 in breast cancer cells leads to a switch from an invasive to a proliferative phenotype as indicated by an increased cell proliferation, reduced adhesion and invasionin vitro, and a reduced capacity to form lung metastasesin vivo.
Systematic review and meta-analysis of Endostar (rh-endostatin) combined with chemotherapy versus chemotherapy alone for treating advanced non-small cell lung cancer
Authors: Biaoxue Rong , Shuanying Yang , Wei Li , Wei Zhang , Zongjuan Ming
Published: August 2012

Background

Many studies have investigated the efficacy of Endostar combined with platinum-based doublet chemotherapy (PBDC) versus PBDC alone for treating advanced non-small cell lung cancer (NSCLC). This study is a meta-analysis of available evidence.

Methods

Fifteen studies reporting Endostar combined with PBDC versus PBDC alone for treating advanced NSCLC were reviewed. Pooled odds ratios and hazard ratio with 95% confidence intervals were calculated using either the fixed effects model or random effects model.

Results

The overall response rate (ORR) and disease control rate (DCR) of Endostar combined with PBDC for treating NSCLC were significantly higher than those of PBDC alone, with 14.7% and 13.5% improvement, respectively (P < 0.00001). In addition, the time to progression (TTP) and quality of life (QOL) were improved after the treatment of Endostar combined with PBDC (P < 0.00001). The main adverse effects found in this review were hematological reactions, hepatic toxicity, and nausea/vomiting. Endostar combined with PBDC had a similar incidence of adverse reactions compared with PBDC alone (P < 0.05).

Conclusions

Endostar combined with PBDC was associated with higher RR, DCR, and TTP as well as superior QOL profiles compared with PBDC alone. Endostar combined with PBDC had a similar incidence of adverse reactions compared with PBDC alone.
Development and evaluation of an immunochromatographic strip for rapid detection of porcine hemagglutinating encephalomyelitis virus
Authors: Chen Keyan , Zhao Kui , Song Deguang , He Wenqi , Gao Wei , Zhao Chuanbo , Wang Chengli , Gao Feng
Published: August 2012

Background

The incidence of PHE among pigs in many countries is on the rise, and it has caused great economic losses to the pig industry. Therefore, the development of a sensitive, specific, and easily-performed assay is crucial for the rapid detection and surveillance of PHE-CoV infection and transmission.

Results

An immunochromatographic strip was developed for the detection of PHE-CoV. The colloidal gold-labeled MAb 4D4 was used as the detection reagent, and the MAb 1E2 and goat anti-mouse IgG coated the strip's test and control lines, respectively. The immunochromatographic strip was capable of specifically detecting PHE-CoV with a HA unit of 2 within 10 min. Storage of the strips at room temperature for 6 months or at 4°C for 12 months did not change their sensitivity or specificity. Using RT-PCR as a reference test, the relative specificity and sensitivity of the immunochromatographic strip were determined to be 100% and 97.78%, respectively. There was an excellent agreement between the results obtained by RT-PCR and the immunochromatographic strips (kappa = 0.976). Additionally, there was a strong agreement between the sandwich enzyme-linked immunosorbent assay (ELISA) and immunochromatographic strips (Kappa = 0.976). When the immunochromatographic strips were used for diagnosing PHE-CoV infection in the Jilin Province, the PHE-CoV-positive rate ranged from 61.54% in the Jilin district to 17.95% in the Songyuan district.

Conclusions

Based on its high specificity, sensitivity, and stability, the immunochromatographic strip would be suitable for on-site detection of PHE-CoV for surveillance and epidemiological purposes.
β-catenin/Wnt signaling controls progenitor fate in the developing and regenerating zebrafish retina
Authors: Meyers Jason R , Hu Lily , Moses Ariel , Kaboli Kavon , Papandrea Annemarie , Raymond Pamela A
Published: August 2012

Background

The zebrafish retina maintains two populations of stem cells: first, the germinal zone or ciliary marginal zone (CMZ) contains multipotent retinal progenitors that add cells to the retinal periphery as the fish continue to grow; second, radial glia (Müller cells) occasionally divide asymmetrically to generate committed progenitors that differentiate into rod photoreceptors, which are added interstitially throughout the retina with growth. Retinal injury stimulates Müller glia to dedifferentiate, re-enter the cell cycle, and generate multipotent retinal progenitors similar to those in the CMZ to replace missing neurons. The specific signals that maintain these two distinct populations of endogenous retinal stem cells are not understood.

Results

We used genetic and pharmacological manipulation of the β-catenin/Wnt signaling pathway to show that it is required to maintain proliferation in the CMZ and that hyperstimulation of β-catenin/Wnt signaling inhibits normal retinal differentiation and expands the population of proliferative retinal progenitors. To test whether similar effects occur during regeneration, we developed a method for making rapid, selective photoreceptor ablations in larval zebrafish with intense light. We found that dephosphorylated β-catenin accumulates in Müller glia as they re-enter the cell cycle following injury, but not in Müller glia that remain quiescent. Activation of Wnt signaling is required for regenerative proliferation, and hyperstimulation results in loss of Müller glia from the INL as all proliferative cells move into the ONL.

Conclusions

β-catenin/Wnt signaling is thus required for the maintenance of retinal progenitors during both initial development and lesion-induced regeneration, and is sufficient to prevent differentiation of those progenitors and maintain them in a proliferative state. This suggests that the β-catenin/Wnt cascade is part of the shared molecular circuitry that maintains retinal stem cells for both homeostatic growth and epimorphic regeneration.
Very high gravity ethanol fermentation by flocculating yeast under redox potential-controlled conditions
Authors: Liu Chen-Guang , Wang Na , Lin Yen-Han , Bai Feng-Wu
Published: August 2012

Background

Very high gravity (VHG) fermentation using medium in excess of 250 g/L sugars for more than 15% (v) ethanol can save energy consumption, not only for ethanol distillation, but also for distillage treatment; however, stuck fermentation with prolonged fermentation time and more sugars unfermented is the biggest challenge. Controlling redox potential (ORP) during VHG fermentation benefits biomass accumulation and improvement of yeast cell viability that is affected by osmotic pressure and ethanol inhibition, enhancing ethanol productivity and yield, the most important techno-economic aspect of fuel ethanol production.

Results

Batch fermentation was performed under different ORP conditions using the flocculating yeast and media containing glucose of 201 ± 3.1, 252 ± 2.9 and 298 ± 3.8 g/L. Compared with ethanol fermentation by non-flocculating yeast, different ORP profiles were observed with the flocculating yeast due to the morphological change associated with the flocculation of yeast cells. When ORP was controlled at −100 mV, ethanol fermentation with the high gravity (HG) media containing glucose of 201 ± 3.1 and 252 ± 2.9 g/L was completed at 32 and 56 h, respectively, producing 93.0 ± 1.3 and 120.0 ± 1.8 g/L ethanol, correspondingly. In contrast, there were 24.0 ± 0.4 and 17.0 ± 0.3 g/L glucose remained unfermented without ORP control. As high as 131.0 ± 1.8 g/L ethanol was produced at 72 h when ORP was controlled at −150 mV for the VHG fermentation with medium containing 298 ± 3.8 g/L glucose, since yeast cell viability was improved more significantly.

Conclusions

No lag phase was observed during ethanol fermentation with the flocculating yeast, and the implementation of ORP control improved ethanol productivity and yield. When ORP was controlled at −150 mV, more reducing power was available for yeast cells to survive, which in turn improved their viability and VHG ethanol fermentation performance. On the other hand, controlling ORP at −100 mV stimulated yeast growth and enhanced ethanol production under the HG conditions. Moreover, the ORP profile detected during ethanol fermentation with the flocculating yeast was less fluctuated, indicating that yeast flocculation could attenuate the ORP fluctuation observed during ethanol fermentation with non-flocculating yeast.
Neuroprotective effect of an angiotensin receptor type 2 agonist following cerebral ischemia in vitro and in vivo
Authors: Lee Seyoung , Brait Vanessa H , Arumugam Thiruma V , Evans Megan A , Kim Hyun Ah , Widdop Robert E , Drummond Grant R , Sobey Christopher G , Jones Emma S
Published: August 2012

Background

Intracerebral administration of the angiotensin II type 2 receptor (AT2R) agonist, CGP42112, is neuroprotective in a rat model of ischemic stroke. To explore further its possible cellular target(s) and therapeutic utility, we firstly examined whether CGP42112 may exert direct protective effects on primary neurons following glucose deprivation in vitro. Secondly, we tested whether CGP42112 is effective when administered systemically in a mouse model of cerebral ischemia.

Methods

Primary cortical neurons were cultured from E17 C57Bl6 mouse embryos for 9 d, exposed to glucose deprivation for 24 h alone or with drug treatments, and percent cell survival assessed using trypan blue exclusion. Ischemic stroke was induced in adult male C57Bl6 mice by middle cerebral artery occlusion for 30 min, followed by reperfusion for 23.5 h. Neurological assessment was performed and then mice were euthanized and infarct and edema volume were analysed.

Results

During glucose deprivation, CGP42112 (1x10-8 M and 1x10-7 M) reduced cell death by ~30%, an effect that was prevented by the AT2R antagonist, PD123319 (1x10-6 M). Neuroprotection by CGP42112 was lost at a higher concentration (1x10-6 M) but was unmasked by co-application with the AT1R antagonist, candesartan (1x10-7 M). By contrast, Compound 21 (1x10-8 M to 1x10-6 M), a second AT2R agonist, had no effect on neuronal survival. Mice treated with CGP42112 (1 mg/kg i.p.) after cerebral ischemia had improved functional outcomes over vehicle-treated mice as well as reduced total and cortical infarct volumes.

Conclusions

These results indicate that CGP42112 can directly protect neurons from ischemia-like injury in vitro via activation of AT2Rs, an effect opposed by AT1R activation at high concentrations. Furthermore, systemic administration of CGP42112 can reduce functional deficits and infarct volume following cerebral ischemia in vivo.
Red blood cell ATP/ADP & nitric oxide: The best vasodilators in diabetic patients
Authors: Bakhtiari Nuredin , Hosseinkhani Saman , Larijani Bagher , Mohajeri-Tehrani Mohammad Reza , Fallah Abbas
Published: August 2012

Background

Diabetes mellitus is a group of metabolic diseases characterized by high blood sugar (glucose) levels that result from defects in insulin secretion, or action, or both. Inspired by previous report the release of ATP from RBCs, which may participate in vessel dilation by stimulating NO production in the endothelium through purinergic receptor signaling and so, the aim of this study is to clearly determined relationship between RBC ATP/ADP ratio with nitric oxide.

Methods

The ATP/ADP ratio of erythrocytes among four groups of normal individuals (young & middle age), athletes’ subjects and diabetic patients were compared and the relationship between ATP/ADP ratio and NO level of plasma was determined with AVOVA test and bioluminescence method.

Results

ATP/ADP level in four groups normal (young & middle age), athletes, diabetes] are measured and analyzed with ANOVA test that show a significant difference between groups (P-value < 0.001). A significant positive correlation was found between RBC ATP/ADP content (r = 0.705; P < 0.001). Plasma NO content is also analyzed with ANOVA test which shows a significant difference between groups.

Conclusion

In this study, a positive relationship between RBC ATP/ADP ratio and NO was found. Based on the obtained result, higher RBC ATP/ADP content may control the ratio of plasma NO in different individuals, also this results show that ATP can activate endothelial cells in NO production and is a main factor in releasing of NO from endothelial cells.
Antinociceptive interaction of (±)-CPP and propentofylline in monoarthritic rats
Authors: Morales Francisco , Constandil Luis , Pelissier Teresa , Hernández Alejandro , Laurido Claudio
Published: August 2012

Introduction

Multiple studies have shown that glial cells of the spinal cord, such as astrocytes and microglia, have close contact with neurons, suggesting the term tripartite synapse. In these synapses, astrocytes surrounding neurons contribute to neuronal excitability and synaptic transmission, thereby increasing nociception and thus the persistence of chronic pain. Conversely, the N-methyl-D-aspartate (NMDA) receptor is crucial in the generation and maintenance of chronic pain. It has multiple sites of modulation. One is the site of recognition of extracellular neurotransmitter (glutamate), which can be blocked by competitive antagonists such as (3-(2-carboxipiperazin-4)1-propyl phosphonic acid), (±)-CPP, resulting in a blockade of the calcium current and thus the intracellular transduction process. In the present study, we investigated whether the potential antinociceptive effect of glial inhibition produced by propentofylline (PPF) can be enhanced when combined with an NMDA-receptor inhibitor such as (±)-CPP.

Methods

We used Sprague-Dawley monoarthritic rats. The monoarthritis was induced by injection of complete Freund adjuvant in the right tibiotarsal joint. Four weeks later, rats were treated with PPF (1, 10, 30, and 100 μg/10 μl) intrathecally (i.t.) for 10 days, injected once with (±)-CPP (2.5, 5, 12.5, 25, 50, and 100 μg/10 μl, i.t.), or both treatments combined. The antinociceptive effect was evaluated on day 11 for PPF and immediately to (±)-CPP, by assessing the vocalization threshold to mechanical stimulation of the arthritic paw.

Results

The data indicate that intrathecal administration of increasing concentrations of (±)-CPP or PPF produced a significant dose-dependent antinociceptive effect with respect to monoarthritic rats receiving saline. The linear regression analysis showed that the dose that produces 30% of maximal effect (ED30) for i.t. (±)-CPP was 3.97 μg, and 1.42 μg for i.t. PPF. The administration of the PPF and (±)-CPP combination in fixed proportions of ED30 produced a dose-dependent antinociceptive effect, showing an interaction of the supraadditive type.

Conclusions

The results suggest that glia inhibitors can synergically potentiate the effect of glutamate blockers for the treatment of chronic inflammatory pain.
Shifted-elliptical nonstretch moveout correction of wide-angle seismic data in the τ-p domain, using an example from the Faeroe-Shetland Basin
Authors: Masoomzadeh Hassan , Singh Satish C. , Barton Penny J.
Published: August 2012

We developed a method of moveout correction in the [Formula: see text] domain to tackle some of the problems associated with processing wide-angle seismic reflection data, including residual moveout and normal-moveout stretching. We evaluated the concept of the shifted ellipse in the [Formula: see text] domain as an alternative to the well-known concept of the shifted hyperbola in the [Formula: see text] domain. We used this shifted-ellipse concept to address the problem of residual moveout caused by vertical heterogeneity in the subsurface. We also addressed the stretching problem associated with dynamic corrections by combining selected strips from a set of constant-moveout stacks generated using a shifted-ellipse equation. Application of this method to a wide-angle data set from the Faeroe-Shetland Basin provided an enhanced image of the subbasalt structure.
A model-compression scheme for nonlinear electromagnetic inversions
Authors: Abubakar A. , Habashy T. M. , Lin Y. , Li M.
Published: August 2012

We have developed a model-compression scheme for improving the efficiency of the regularized Gauss-Newton inversion algorithm for marine controlled-source electromagnetic applications. In this scheme, the unknown model parameters (the conductivity/resistivity distribution) are represented in terms of a basis such as Fourier and wavelet (Haar and Daubechies). By applying a truncation criterion, the model may then be approximated by a reduced number of basis functions, which is usually much less than the number of the model parameters. Further, because the controlled-source electromagnetic measurements have low resolution, it is sufficient for inversion to only keep the low-spatial-frequency part of the image. This model-compression scheme accelerates the computational time and also reduces the memory usage of the Gauss-Newton method. We are able to significantly reduce the algorithm computational complexity without compromising the quality of the inverted models.
Frontal information flow and connectivity in
psychopathy
Authors: Yang Yaling , Raine Adrian , Joshi Anand A. , Joshi Shantanu , Chang Yu-Teng , Schug Robert A. , Wheland David , Leahy Richard , Narr Katherine L.
Published: August 2012

Despite accumulating evidence of structural deficits in individuals with
psychopathy, especially in frontal regions, our understanding of
systems-level disturbances in cortical networks remains limited. We applied
novel graph theory-based methods to assess information flow and connectivity
based on cortical thickness measures in 55 individuals with psychopathy and
47 normal controls. Compared with controls, the psychopathy group showed
significantly altered interregional connectivity patterns. Furthermore,
bilateral superior frontal cortices in the frontal network were identified
as information flow control hubs in the psychopathy group in contrast to
bilateral inferior frontal and medial orbitofrontal cortices as network hubs
of the controls. Frontal information flow and connectivity may have a
significant role in the neuropathology of psychopathy.
Loss of MMP-2 in osteoblasts upregulates osteopontin and bone sialoprotein expression in a circuit regulating bone homeostasis
Authors: Mosig Rebecca A. , Martignetti John A.
Published: August 2012

Multicentric osteolysis with arthropathy (MOA; MIM 605156) is an inherited osteolyses/arthritis syndrome resulting from loss of MMP-2. We recently demonstrated that Mmp2-/- mice represent a unique model for the study of the human disease, sharing many features of the human syndrome including skeletal dysplasia and defects in osteoblast behavior. We therefore sought to explore, secondary molecular effects of MMP-2 loss which coexist with the underlying skeletal and osteoblast phenotypes. We used quantitative real-time PCR to measure osteoblast-related gene expression through ex vivo osteoblast differentiation of bone marrow stromal cells (BMSC) from Mmp2-/- and Mmp2+/+ mice. We used western blot to measure OPN serum levels and immunohistochemical staining to examine bone expression. MMP-2 expression was inhibited in SaOS2 cells using siRNA and decreased MMP-2 RNA and protein expression was confirmed by qRT-PCR and western blot, respectively. Mmp2-/- BMSC induced to differentiate into osteoblasts were shown to significantly upregulate OPN and BSP expression levels when compared to controls. Transcriptional upregulation is maintained in vivo as demonstrated by increased levels of OPN in serum and bone in Mmp2-/- mice. Finally, these effects are generalizable since siRNA-mediated inhibition in cultured cells also upregulates OPN and BSP. OPN and BSP were known to affect MMP-2 expression and activity but had not previously been shown to be regulated by MMP-2. Identification of this novel circuitry provides insight into the potential molecular landscape underlying the MOA phenotype and highlights a pathway that may play a role in normal bone homeostasis.