bluuebunny/crossref_metadata_2025 huggingface dataset:
Recent Advances in Low-Temperature Deposition Methods of Transparent, Photocatalytic TiO2 Coatings on Polymers
Authors: Watté Jonathan , Van Zele Matthias , De Buysser Klaartje , Van Driessche Isabel
Published: April 2018

In this paper, we present an overview as well as current advances in the low-temperature deposition of highly crystalline suspensions of titania nanoparticles on polymers for photocatalytic applications. The presence of preformed titania nanoparticles yields the possibility of producing photocatalytically active coatings at reduced temperatures. Transparent and photocatalytically active TiO2 coatings that degrade organic matter, have been widely applied to bestow self-cleaning properties onto surfaces. This low-temperature deposition method and its transition to polymers would open an entire array of possible self-cleaning applications. During this research, incorporation of a silica buffer layer was applied to improve the compatibility of the inorganic coating on a substrate, such as polymethylmethacrylate (PMMA) and polyphenylsulphone (PPSU). The photocatalytic activity of the obtained coating was analyzed for its photocatalytic abilities by evaluating the color removal of a dye solution (methylene blue, MB) under UV irradiation and compared with commercial Pilkington Activ® self-cleaning glass. Our results indicate that the titania-coated silica-polymer systems yield a higher photocatalytic activity towards the degradation of organic pollutants. This method proves that the deposition of highly crystalline anatase suspensions on silica buffer layers is a viable method to produce photocatalytic coatings on heat-sensitive substrates.
Radiation Damage in Macromolecular Crystallography—An Experimentalist’s View
Authors: Taberman Helena
Published: April 2018

Radiation damage still remains a major limitation and challenge in macromolecular X-ray crystallography. Some of the high-intensity radiation used for diffraction data collection experiments is absorbed by the crystals, generating free radicals. These give rise to radiation damage even at cryotemperatures (~100 K), which can lead to incorrect biological conclusions being drawn from the resulting structure, or even prevent structure solution entirely. Investigation of mitigation strategies and the effects caused by radiation damage has been extensive over the past fifteen years. Here, recent understanding of the physical and chemical phenomena of radiation damage is described, along with the global effects inflicted on the collected data and the specific effects observed in the solved structure. Furthermore, this review aims to summarise the progress made in radiation damage studies in macromolecular crystallography from the experimentalist’s point of view and to give an introduction to the current literature.
(BEDT-TTF)2Cu2(CN)3 Spin Liquid: Beyond the Average Structure
Authors: Foury-Leylekian Pascale , Ilakovac Vita , Balédent Victor , Fertey Pierre , Arakcheeva Alla , Milat Ognjen , Petermann Denis , Guillier Gilles , Miyagawa Kazuya , Kanoda Kazushi , Alemany Pere , Canadell Enric , Tomic Silvia , Pouget Jean-Paul
Published: April 2018

We present here the first accurate determination of the exact structure of κ-(BEDT-TTF)2Cu2(CN)3. Not only did we show that the room temperature structure used over the last twenty years was incorrect, but we were also able to correctly and precisely determine it. The results of our work provide evidence that the structure presents a triclinic symmetry with two non-equivalent dimers in the unit cell, which implies a charge disproportionation between the dimers. However, structural refinement shows that the charge disproportionation is quite weak at room temperature.
Crime Statistics: Modeling Theft in Favour of Victims’ Choices
Authors: Idsø Johannes , Årethun Torbjørn
Published: April 2018

The number of reported property thefts has dropped steeply in many European countries over the last 15 years. One reason for this could be that people have become more honest, which would imply that fewer resources should be allocated to the police and to crime prevention measures. In this paper, we have elaborated upon some alternative explanatory factors behind the decrease in the number of reported crimes within a utility-maximizing model where both thefts and victims are behaving rationally. Increased time and travelling costs for reporting, economic growth and a lower rate of solving crime could all explain the development in the reported crime rate. Within this theoretical approach, the nominal crime rate could decrease, while the real crime rate either remains constant or even increases. Applying the number of reported thefts as the sole or main parameter for allocating resources for crime prevention measures may result in a sub-optimal resource allocation. Developments in the number of reported thefts must therefore be supplemented by other indicators to provide a better basis to ensure an optimal allocation of police resources to combat theft. Estimates of unreported crimes must also be included as part of the basis of these allocation decisions.
Robust Offset-Free Speed Tracking Controller of Permanent Magnet Synchronous Generator for Wind Power Generation Applications
Authors: Kim Seok-Kyoon , Lee Kyo-Beum
Published: April 2018

This article presents a robust speed tracking algorithm for a permanent magnet synchronous generator utilized for wind power generation systems, considering the machine parameter and load uncertainties. There are two major contributions: (a) a disturbance observer is designed to exponentially estimate disturbances from the model-plant mismatches and severe load torque variations, and (b) it is included in a nonlinear cascade-type proportional speed tracking controller to establish the performance recovery and offset-free properties without the use of tracking error integrators. A simulation result numerically verifies the effectiveness of the proposed technique, where the PowerSIM software emulates a wind power generation system.
Nitrogen Doped Ordered Mesoporous Carbon as Support of PtRu Nanoparticles for Methanol Electro-Oxidation
Authors: Sebastián David , Nieto-Monge María , Pérez-Rodríguez Sara , Pastor Elena , Lázaro María
Published: April 2018

The low oxidation kinetics of alcohols and the need for expensive platinum group metals are still some of the main drawbacks for the commercialization of energy efficient direct alcohol fuel cells. In this work, we investigate the influence of nitrogen doping of ordered mesoporous carbon (CMK) as support on the electrochemical activity of PtRu nanoparticles. Nitrogen doping procedures involve the utilization of pyrrole as both nitrogen and carbon precursor by means of a templating method using mesoporous silica. This method allows obtaining carbon supports with up to 14 wt. % nitrogen, with an effective introduction of pyridinic, pyrrolic and quaternary nitrogen. PtRu nanoparticles were deposited by sodium formate reduction method. The presence of nitrogen mainly influences the Pt:Ru atomic ratio at the near surface, passing from 50:50 on the bare (un-doped) CMK to 70:30 for the N-doped CMK catalyst. The electroactivity towards the methanol oxidation reaction (MOR) was evaluated in acid and alkaline electrolytes. The presence of nitrogen in the support favors a faster oxidation of methanol due to the enrichment of Pt at the near surface together with an increase of the intrinsic activity of PtRu nanoparticles.
Experimental Study on Horizontal Cylinders with Triangular Fins under Natural Convection
Authors: Lee Gu-Won , Kim Hyun , Kim Dong-Kwon
Published: April 2018

In this study, thermal resistances of horizontal cylinders with triangular fins were measured in regard to fin numbers, fins heights, and temperature differences. Thereafter, an empirical correlation was proposed and validated for predicting the Nusselt numbers under the following conditions: Rayleigh number, 200,000–1,000,000; fin aspect ratio, 1.6–5.0; and fin number, 9–72. Finally, with the proposed correlation, the effects of fin numbers, fins heights, and fin thicknesses on the thermal resistances of the horizontal cylinders with triangular fins were investigated. It was shown that the thermal resistance generally increases as the fin number, fin height, and fin thickness increase. It is expected that horizontal cylinders for various cooling devices with triangular fins can be designed based on the findings of the present study.
A Unique Failure Mechanism in the Nexus 6P Lithium-Ion Battery
Authors: Saxena Saurabh , Xing Yinjiao , Pecht Michael
Published: April 2018

Nexus 6P smartphones have been beset by battery drain issues, which have been causing premature shutdown of the phone even when the charge indicator displays a significant remaining runtime. To investigate the premature battery drain issue, two Nexus 6P smartphones (one new and one used) were disassembled and their batteries were evaluated using computerized tomography (CT) scan analysis, electrical performance (capacity, resistance, and impedance) tests, and cycle life capacity fade tests. The “used” smartphone battery delivered only 20% of the rated capacity when tested in a first capacity cycle and then 15% of the rated capacity in a second cycle. The new smartphone battery exceeded the rated capacity when first taken out of the box, but exhibited an accelerated capacity fade under C/2 rate cycling and decreased to 10% of its initial capacity in just 50 cycles. The CT scan results revealed the presence of contaminant materials inside the used battery, raising questions about the quality of the manufacturing process.
Grey Wolf Optimization-Based Optimum Energy-Management and Battery-Sizing Method for Grid-Connected Microgrids
Authors: Nimma Kutaiba Sabah , Al-Falahi Monaaf D. A. , Nguyen Hung Duc , Jayasinghe S. D. G. , Mahmoud Thair S. , Negnevitsky Michael
Published: April 2018

In the revolution of green energy development, microgrids with renewable energy sources such as solar, wind and fuel cells are becoming a popular and effective way of controlling and managing these sources. On the other hand, owing to the intermittency and wide range of dynamic responses of renewable energy sources, battery energy-storage systems have become an integral feature of microgrids. Intelligent energy management and battery sizing are essential requirements in the microgrids to ensure the optimal use of the renewable sources and reduce conventional fuel utilization in such complex systems. This paper presents a novel approach to meet these requirements by using the grey wolf optimization (GWO) technique. The proposed algorithm is implemented for different scenarios, and the numerical simulation results are compared with other optimization methods including the genetic algorithm (GA), particle swarm optimization (PSO), the Bat algorithm (BA), and the improved bat algorithm (IBA). The proposed method (GWO) shows outstanding results and superior performance compared with other algorithms in terms of solution quality and computational efficiency. The numerical results show that the GWO with a smart utilization of battery energy storage (BES) helped to minimize the operational costs of microgrid by 33.185% in comparison with GA, PSO, BA and IBA.
Tropical Tree Species Effects on Soil pH and Biotic Factors and the Consequences for Macroaggregate Dynamics
Authors: Russell Ann , Kivlin Stephanie , Hawkes Christine
Published: April 2018

Physicochemical and biotic factors influence the binding and dispersivity of soil particles, and thus control soil macroaggregate formation and stability. Although soil pH influences dispersivity, it is usually relatively constant within a site, and thus not considered a driver of aggregation dynamics. However, land-use change that results in shifts in tree-species composition can result in alteration of soil pH, owing to species-specific traits, e.g., support of nitrogen fixation and Al accumulation. In a long-term, randomized complete block experiment in which climate, soil type, and previous land-use history were similar, we evaluated effects of individual native tropical tree species on water-stable macroaggregate size distributions in an Oxisol. We conducted this study at La Selva Biological Station in Costa Rica, in six vegetation types: 25-year-old plantations of four tree species grown in monodominant stands; an unplanted Control; and an adjacent mature forest. Tree species significantly influenced aggregate proportions in smaller size classes (0.25–1.0 mm), which were correlated with fine-root growth and litterfall. Tree species altered soil pH differentially. Across all vegetation types, the proportion of smaller macroaggregates declined significantly as soil pH increased (p ≤ 0.0184). This suggests that alteration of pH influences dispersivity, and thus macroaggregate dynamics, thereby playing a role in soil C, N, and P cycling.
Development of a Social DTN for Message Communication between SNS Group Members
Authors: Takasuka Hidenori , Hirai Koichi , Takami Kazumasa
Published: April 2018

Smartphones have the ability to communicate with other terminals through ad hoc connections. A variety of applications have been developed to exploit this ability. The authors have developed an Android OS (operating system) application (called “social DTN manager”) that builds a DTN (delay, disruption, disconnection tolerant networking) among members of a social networking service (SNS) community using a community token. The members can exchange messages over this network. Control messages for building a DTN are forwarded to only those nodes that use the same community token in order to reduce flooding of message copies. When a source node sends a communication request to its destination node, they exchange control messages to establish a communication route. Relay nodes use these messages to create and hold routing information for these nodes in their routing tables. Thereafter, relay nodes can forward data messages based on their routing tables. This again reduces flooding of message copies. The social DTN manager incorporates these functions, Facebook Graph API and Google Nearby Connections API. The authors have installed it in Android terminals and confirmed that a social DTN can successfully be built using this application and that data messages can be exchanged between terminals via reactive routes.
Hot Coronae in Local AGN: Present Status and Future Perspectives
Authors: Marinucci Andrea , Tamborra Francesco , Bianchi Stefano , Dovčiak Michal , Matt Giorgio , Middei Riccardo , Tortosa Alessia
Published: April 2018

The nuclear X-ray emission in radio-quiet Active Galactic Nuclei (AGN) is commonly believed to be due to inverse Compton scattering of soft UV photons in a hot corona. The radiation is expected to be polarized, the polarization degree depending mainly on the geometry and optical depth of the corona. Nuclear Spectroscopic Telescope Array (NuSTAR) observations are providing for the first time high quality measurements of the coronal physical parameters—temperature and optical depth. We hereby review the NuSTAR results on the coronal physical parameters (temperature and optical depth) and discuss their implications for future X-ray polarimetric studies.
Coastal Evolution, Hydrothermal Migration Pathways and Soft Deformation along the Campania Continental Shelf (Southern Tyrrhenian Sea): Insights from High-Resolution Seismic Profiles
Authors: Misuraca Martina , Budillon Francesca , Tonielli Renato , Di Martino Gabriella , Innangi Sara , Ferraro Luciana
Published: April 2018

A closely spaced set of high-resolution Chirp-Sonar and Sparker profiles and swath bathymetric data was acquired in 2013 for the I-AMICA Project off the Volturno River mouth (Southern Tyrrhenian Sea) by the Istituto per l’Ambiente Marino Costiero (IAMC), National Research Council of Italy (CNR). The palaeo-topography of three key surfaces, represented by the bounding surfaces of the post-glacial lithosomes, was mapped by the interpolation of seismically detected reflectors. The morphology of the surface related to the Last Glacial Maximum (LGM) regression revealed the presence of fault linkages which defined a small-scale accommodation zone with an E–W trending interbasinal relative high. The observed set of oppositely dipping faults, NNW- and ENE-directed, locally controlled the deposition of the paralic/deltaic bodies during the post-glacial rise in sea level, as testified by their wedge-shaped geometries and shifting depocentres. The deformation may be linked to the Campi Flegrei caldera collapse following the Neapolitan Yellow Tuff (NYT) eruption and aged 15 ka BP. The relevant thickness of the Transgressive System Tract (TST) testifies to an increased sediment yield and intense reworking in coastal areas, probably driven by the high volcanoclastic supply during volcanic paroxysm, almost coeval with the post-glacial transgression. Fluid escape features linked to an E–W striking fluid front at the outer shelf suggest the presence of an hydrothermal system controlled by the predominant direction of normal to oblique Quaternary-active faults and by lithologic discontinuities across the sedimentary pile.
Membrane Separation of Ammonium Bisulfate from Ammonium Sulfate in Aqueous Solutions for CO2 Mineralisation
Authors: Koivisto Evelina , Zevenhoven Ron
Published: April 2018

The separation of ammonium bisulfate (ABS) from ammonium sulfate (AS) in aqueous solutions by monovalent ion selective membranes was studied. Optimised usage of these chemicals is both an important and challenging step towards a more efficient CO2 mineralisation process route developed at Åbo Akademi University (ÅA). The membranes were placed in a three or five-compartment electrodialysis stack. Silver, stainless steel and platinum electrodes were tested, of which a combination of Pt (anode) and stainless steel (cathode) electrodes were found to be most suitable. Separation efficiencies close to 100% were reached based on ABS concentrations in the feed solution. The tests were performed with an initial voltage of either 10 V–20 V, but limitations in the electrical power supply equipment eventually resulted in a voltage drop as separation proceeded. Exergy calculations for energy efficiency assessment show that the input exergy (electrical power) is many times higher than the reversible mixing exergy, which indicates that design modifications must be made. Further work will focus on the possibilities to make the separation even more efficient and to develop the analysis methods, besides the use of another anode material.
The Relationship of 5-Aminolevulinic Acid on Mood and Coping Ability in Prediabetic Middle Aged and Older Adults
Authors: Aquino Rachael K. , Perez Michael , Sil Payel , Shintani Terry , Harrigan Rosanne , Rodriguez Beatriz
Published: April 2018

In 2010, approximately 79 million Americans had prediabetes and about 50 percent of those individuals were 65 years and older. The most effective diabetes prevention method in prediabetic adults is lifestyle modification. However, despite the benefits of lifestyle change, diabetes prevalence continues to increase. Maintaining a regular exercise routine and a healthy eating plan may be difficult because of the negative emotional barriers (i.e., stress, mood) that a prediabetic individual faces. This is particularly evident in older individuals when you combine that with decreases in mobility and geriatric syndromes. A potential treatment for these emotional barriers is a natural supplement called 5-aminolevulinic acid (5-ALA). In the current study, the group included 154 participants, both men and women, ranging between the ages of 41 to 71 years old. The study design was a double-blind, randomized parallel-group study. The Psychosocial Depressive Symptoms Questionnaire (PDS) and the Perceived Stress Scale (PSS) were used to examine the effect of two doses of 5-ALA (15 mg and 50 mg) on various components of mood (i.e., hopefulness, loneliness, and motivation) and coping ability. Using SAS software, an ordered logistic regression model was used to analyze the association between the dose groups (control, 15 mg, and 50 mg) and the responses to the two questionnaires, the PDS and PSS, used in this study. An integrative literature review, using the PubMed database, searched for studies on the relationship between 5-ALA administration and mood and coping ability. Our literature review resulted in zero published articles. Next, we found that the intake of 5-ALA was significantly associated with improved coping ability (p = 0.004) and improved self-perception of effort spent (p = 0.002). Finally, we found a significant dose-dependent relationship for the association of 5-ALA intake on measures of effort (p = 0.003), loneliness (p = 0.006), and coping ability (p = 0.003). The 50 mg dose was more effective than the 15 mg dose in improving these measures. In conclusion, after 12 weeks of taking 5-ALA, we found significant improvements in self-perception of effort spent, loneliness, and coping ability in prediabetic middle age and older adults. Improved mood and coping ability may allow prediabetic individuals to overcome the emotional obstacles preventing them from maintaining a healthy lifestyle and ultimately, help them to avoid the development of diabetes.
The Political Economy of Health Co-Benefits: Embedding Health in the Climate Change Agenda
Authors: Workman Annabelle , Blashki Grant , Bowen Kathryn J. , Karoly David J. , Wiseman John
Published: April 2018

A complex, whole-of-economy issue such as climate change demands an interdisciplinary, multi-sectoral response. However, evidence suggests that human health has remained elusive in its influence on the development of ambitious climate change mitigation policies for many national governments, despite a recognition that the combustion of fossil fuels results in pervasive short- and long-term health consequences. We use insights from literature on the political economy of health and climate change, the science–policy interface and power in policy-making, to identify additional barriers to the meaningful incorporation of health co-benefits into climate change mitigation policy development. Specifically, we identify four key interrelated areas where barriers may exist in relation to health co-benefits: discourse, efficiency, vested interests and structural challenges. With these insights in mind, we argue that the current politico-economic paradigm in which climate change is situated and the processes used to develop climate change mitigation policies do not adequately support accounting for health co-benefits. We present approaches for enhancing the role of health co-benefits in the development of climate change mitigation policies to ensure that health is embedded in the broader climate change agenda.
Public Health and Unconventional Oil and Gas Extraction Including Fracking: Global Lessons from a Scottish Government Review
Authors: Watterson Andrew , Dinan William
Published: April 2018

Unconventional oil and gas extraction (UOGE) including fracking for shale gas is underway in North America on a large scale, and in Australia and some other countries. It is viewed as a major source of global energy needs by proponents. Critics consider fracking and UOGE an immediate and long-term threat to global, national, and regional public health and climate. Rarely have governments brought together relatively detailed assessments of direct and indirect public health risks associated with fracking and weighed these against potential benefits to inform a national debate on whether to pursue this energy route. The Scottish government has now done so in a wide-ranging consultation underpinned by a variety of reports on unconventional gas extraction including fracking. This paper analyses the Scottish government approach from inception to conclusion, and from procedures to outcomes. The reports commissioned by the Scottish government include a comprehensive review dedicated specifically to public health as well as reports on climate change, economic impacts, transport, geology, and decommissioning. All these reports are relevant to public health, and taken together offer a comprehensive review of existing evidence. The approach is unique globally when compared with UOGE assessments conducted in the USA, Australia, Canada, and England. The review process builds a useful evidence base although it is not without flaws. The process approach, if not the content, offers a framework that may have merits globally.
A Predictive Model Has Identified Tick-Borne Encephalitis High-Risk Areas in Regions Where No Cases Were Reported Previously, Poland, 1999–2012
Authors: Stefanoff Pawel , Rubikowska Barbara , Bratkowski Jakub , Ustrnul Zbigniew , Vanwambeke Sophie , Rosinska Magdalena
Published: April 2018

During 1999–2012, 77% of the cases of tick-borne encephalitis (TBE) were recorded in two out of 16 Polish provinces. However, historical data, mostly from national serosurveys, suggest that the disease could be undetected in many areas. The aim of this study was to identify which routinely-measured meteorological, environmental, and socio-economic factors are associated to TBE human risk across Poland, with a particular focus on areas reporting few cases, but where serosurveys suggest higher incidence. We fitted a zero-inflated Poisson model using data on TBE incidence recorded in 108 NUTS-5 administrative units in high-risk areas over the period 1999–2012. Subsequently we applied the best fitting model to all Polish municipalities. Keeping the remaining variables constant, the predicted rate increased with the increase of air temperature over the previous 10–20 days, precipitation over the previous 20–30 days, in forestation, forest edge density, forest road density, and unemployment. The predicted rate decreased with increasing distance from forests. The map of predicted rates was consistent with the established risk areas. It predicted, however, high rates in provinces considered TBE-free. We recommend raising awareness among physicians working in the predicted high-risk areas and considering routine use of household animal surveys for risk mapping.
Toenail Chromium Concentration and Metabolic Syndrome among Korean Adults
Authors: Son Jakyung , Morris J. Steven , Park Kyong
Published: April 2018

Although in vivo and in vitro studies have shown that chromium has protective effects against metabolic diseases, few studies have examined this association in humans. The present study examined chronic chromium (Cr) exposure among Koreans based on the measurement of toenail Cr concentrations, and analyzed the associations between toenail Cr concentrations and metabolic syndrome (MetS) and its components. We conducted a cross-sectional analysis using baseline data from the prospective cohort study in the Yeungnam area of South Korea that included 232 men and 268 women. Toenail Cr concentration was quantified by neutron activation analysis, and metabolic biomarker levels were obtained through medical examinations. The odd ratios (OR) of prevalent MetS and its components in correlation with Cr concentrations were calculated using multivariable logistic regression. After multiple confounding variables were adjusted for, participants with higher concentrations of Cr had a prevalence rate of MetS similar to those with lower concentrations (OR, 1.84; 95% confidence interval, 0.65–5.23). Our results do not support an association between long-term exposure to Cr and a lower prevalence of MetS in Koreans, whose Cr concentrations are relatively low compared to those of populations in Europe and the United States.
Assessment of Accuracy in the Identification of Rock Formations from Aerial and Terrestrial Laser-Scanning Data
Authors: Paleček Václav , Kubíček Petr
Published: April 2018

Rock formations are among the most spectacular landscape features both for experts and the public. However, information about these objects is often stored inaccurately in existing spatial databases, their corresponding elevations are missing, or the entire rock object is completely absent. Cartographic depiction is also reduced to a point of areal symbology of a largely generalized character. This paper discusses options in identifying and analyzing rock formations from two digital terrain models (DTMs), DMR 5G and DMR 5G+, and irregularly spaced points of airborne laser-scanning (ALS) data with different point densities. A semi-automatic method allowing rock formations to be identified from DTMs is introduced at the beginning of the paper. A method to evaluate elevation models (volume differences) is subsequently applied and a 3D model of a selected rock object is created from terrestrial laser-scanning data. Finally, positional and volumetric comparisons of that 3D object are performed in 2D, 2.5D, and 3D. The results of the pilot study confirmed that the digital terrain models studied are a reliable source in identifying and updating rock formations using the semi-automatic method introduced. The results show that DMR 5G model quality decreases with increasing fragmentation and relative rock formation height, while the proportion of gross errors increases. The complementary DMR 5G+ is better in terms of location and altitude.
Binding Interactions of Zinc Cationic Porphyrin with Duplex DNA: From B-DNA to Z-DNA
Authors: Qin Tingxiao , Liu Kunhui , Song Di , Yang Chunfan , Zhao Hongmei , Su Hongmei
Published: April 2018

Recognition of unusual left-handed Z-DNA by specific binding of small molecules is crucial for understanding biological functions in which this particular structure participates. Recent investigations indicate that zinc cationic porphyrin (ZnTMPyP4) is promising as a probe for recognizing Z-DNA due to its characteristic chiroptical properties upon binding with Z-DNA. However, binding mechanisms of the ZnTMPyP4/Z-DNA complex remain unclear. By employing time-resolved UV-visible absorption spectroscopy in conjunction with induced circular dichroism (ICD), UV-vis, and fluorescence measurements, we examined the binding interactions of ZnTMPyP4 towards B-DNA and Z-DNA. For the ZnTMPyP4/Z-DNA complex, two coexisting binding modes were identified as the electrostatic interaction between pyridyl groups and phosphate backbones, and the major groove binding by zinc(II) coordinating with the exposed guanine N7. The respective contribution of each mode is assessed, allowing a complete scenario of binding modes revealed for the ZnTMPyP4/Z-DNA. These interaction modes are quite different from those (intercalation and partial intercalation modes) for the ZnTMPyP4/B-DNA complex, thereby resulting in explicit differentiation between B-DNA and Z-DNA. Additionally, the binding interactions of planar TMPyP4 to DNA were also investigated as a comparison. It is shown that without available virtual orbitals to coordinate, TMPyP4 binds with Z-DNA solely in the intercalation mode, as with B-DNA, and the intercalation results in a structural transition from Z-DNA to B-ZNA. These results provide mechanistic insights for understanding ZnTMPyP4 as a probe of recognizing Z-DNA and afford a possible strategy for designing new porphyrin derivatives with available virtual orbitals for the discrimination of B-DNA and Z-DNA.
A Developed NK-92MI Cell Line with Siglec-7neg Phenotype Exhibits High and Sustainable Cytotoxicity against Leukemia Cells
Authors: Huang Chin-Han , Liao Yi-Jen , Fan Ting-Hsi , Chiou Tzeon-Jye , Lin Yen-Hsi , Twu Yuh-Ching
Published: April 2018

Altered sialic acid processing that leads to upregulation of cell surface sialylation is recognized as a key change in malignant tissue glycosylation. This cancer-associated hypersialylation directly impacts the signaling interactions between tumor cells and their surrounding microenvironment, especially the interactions mediated by immune cell surface sialic acid-binding immunoglobulin-like lectins (Siglecs) to relay inhibitory signals for cytotoxicity. First, we obtained a Siglec-7neg NK-92MI cell line, NK-92MI-S7N, by separating a group of Siglec-7neg cell population from an eight-month-long-term NK-92MI in vitro culture by fluorescence-activated cell sorting (FACS). The effect of Siglec-7 loss on NK-92MI-S7N cells was characterized by the cell morphology, proliferation, and cytotoxic activity via FACS, MTS assay, cytotoxic assay, and natural killer (NK) degranulation assay. We found the expression levels of Siglec-7 in NK-92MI were negatively correlated with NK cytotoxicity against leukemia cells. This NK-92MI-S7N cell not only shared very similar phenotypes with its parental cells but also possessed a high and sustainable killing activity. Furthermore, this Siglec-7neg NK line was unexpectedly capable of eliminating a NK-92MI-resistant leukemia cell, THP-1, through enhancing the effector-target interaction. In this study, a NK cell line with high and sustainable cytotoxicity was established and this cell may provide a potential application in NK-based treatment for leukemia patients.
Macrophage Populations in Visceral Adipose Tissue from Pregnant Women: Potential Role of Obesity in Maternal Inflammation
Authors: Bravo-Flores Eyerahi , Mancilla-Herrera Ismael , Espino y Sosa Salvador , Ortiz-Ramirez Marco , Flores-Rueda Verónica , Ibargüengoitia-Ochoa Francisco , Ibañez Carlos , Zambrano Elena , Solis-Paredes Mario , Perichart-Perera Otilia , Sanchez-Martinez Maribel , Medina-Bastidas Diana , Reyes-Muñoz Enrique , Estrada-Gutierrez Guadalupe
Published: April 2018

Obesity is associated with inflammatory changes and accumulation and phenotype polarization of adipose tissue macrophages (ATMs). Obese pregnant women have alterations in adipose tissue composition, but a detailed description of macrophage population is not available. In this study, we characterized macrophage populations in visceral adipose tissue (VAT) from pregnant women with normal, overweight, and obese pregestational weight. Immunophenotyping of macrophages from VAT biopsies was performed by flow cytometry using CD45 and CD14 as markers of hematopoietic and monocyte linage, respectively, while HLA-DR, CD11c, CD163, and CD206 were used as pro- and anti-inflammatory markers. Adipocyte number and size were evaluated by light microscopy. The results show that pregnant women that were overweight and obese during the pregestational period had adipocyte hypertrophy. Two different macrophage populations in VAT were identified: recruited macrophages (CD45+CD14+), and a novel population lacking CD45, which was considered to be a resident macrophages subset (CD45−CD14+). The number of resident HLA−DRlow/− macrophages showed a negative correlation with body mass index (BMI). Both resident and recruited macrophages from obese women expressed higher CD206 levels. CD11c expression was higher in resident HLA-DR+ macrophages from obese women. A strong correlation between CD206 and CD11c markers and BMI was observed. Our findings show that being overweight and obese in the pregestational period is associated with adipocyte hypertrophy and specific ATMs populations in VAT.
The Combinational Use of CRISPR/Cas9 and Targeted Toxin Technology Enables Efficient Isolation of Bi-Allelic Knockout Non-Human Mammalian Clones
Authors: Watanabe Satoshi , Sakurai Takayuki , Nakamura Shingo , Miyoshi Kazuchika , Sato Masahiro
Published: April 2018

Recent advances in genome editing systems such as clustered regularly interspaced short palindromic repeats/CRISPR-associated protein-9 nuclease (CRISPR/Cas9) have facilitated genomic modification in mammalian cells. However, most systems employ transient treatment with selective drugs such as puromycin to obtain the desired genome-edited cells, which often allows some untransfected cells to survive and decreases the efficiency of generating genome-edited cells. Here, we developed a novel targeted toxin-based drug-free selection system for the enrichment of genome-edited cells. Cells were transfected with three expression vectors, each of which carries a guide RNA (gRNA), humanized Cas9 (hCas9) gene, or Clostridium perfringens-derived endo-β-galactosidase C (EndoGalC) gene. Once EndoGalC is expressed in a cell, it digests the cell-surface α-Gal epitope, which is specifically recognized by BS-I-B4 lectin (IB4). Three days after transfection, these cells were treated with cytotoxin saporin-conjugated IB4 (IB4SAP) for 30 min at 37 °C prior to cultivation in a normal medium. Untransfected cells and those weakly expressing EndoGalC will die due to the internalization of saporin. Cells transiently expressing EndoGalC strongly survive, and some of these surviving clones are expected to be genome-edited bi-allelic knockout (KO) clones due to their strong co-expression of gRNA and hCas9. When porcine α-1,3-galactosyltransferase gene, which can synthesize the α-Gal epitope, was attempted to be knocked out, 16.7% and 36.7% of the surviving clones were bi-allelic and mono-allelic knockout (KO) cells, respectively, which was in contrast to the isolation of clones in the absence of IB4SAP treatment. Namely, 0% and 13.3% of the resulting clones were bi-allelic and mono-allelic KO cells, respectively. A similar tendency was seen when other target genes such as DiGeorge syndrome critical region gene 2 and transforming growth factor-β receptor type 1 gene were targeted to be knocked out. Our results indicate that a combination of the CRISPR/Cas9 system and targeted toxin technology using IB4SAP allows efficient enrichment of genome-edited clones, particularly bi-allelic KO clones.
A Comparison of the Effects of FATTY ACID DESATURASE 7 and HYDROPEROXIDE LYASE on Plant–Aphid Interactions
Authors: Li Jiamei , Avila Carlos A. , Tieman Denise M. , Klee Harry J. , Goggin Fiona L.
Published: April 2018

The spr2 mutation in tomato (Solanum lycopersicum), which disrupts function of FATTY ACID DESATURASE 7 (FAD7), confers resistance to the potato aphid (Macrosiphum euphorbiae) and modifies the plant’s C6 volatile profiles. To investigate whether C6 volatiles play a role in resistance, HYDROPEROXIDE LYASE (HPL), which encodes a critical enzyme in C6 volatile synthesis, was silenced in wild-type tomato plants and spr2 mutants. Silencing HPL in wild-type tomato increased potato aphid host preference and reproduction on 5-week old plants but had no influence on 3-week old plants. The spr2 mutation, in contrast, conferred strong aphid resistance at both 3 and 5 weeks, and silencing HPL in spr2 did not compromise this aphid resistance. Moreover, a mutation in the FAD7 gene in Arabidopsis thaliana also conferred resistance to the green peach aphid (Myzus persicae) in a genetic background that carries a null mutation in HPL. These results indicate that HPL contributes to certain forms of aphid resistance in tomato, but that the effects of FAD7 on aphids in tomato and Arabidopsis are distinct from and independent of HPL.
GFRA1: A Novel Molecular Target for the Prevention of Osteosarcoma Chemoresistance
Authors: Kim Mihwa , Kim Dae Joon
Published: April 2018

The glycosylphosphatidylinositol-linked GDNF (glial cell derived neurotrophic factor) receptor alpha (GFRA), a coreceptor that recognizes the GDNF family of ligands, has a crucial role in the development and maintenance of the nervous system. Of the four identified GFRA isoforms, GFRA1 specifically recognizes GDNF and is involved in the regulation of proliferation, differentiation, and migration of neuronal cells. GFRA1 has also been implicated in cancer cell progression and metastasis. Recent findings show that GFRA1 can contribute to the development of chemoresistance in osteosarcoma. GFRA1 expression was induced following treatment of osteosarcoma cells with the popular anticancer drug, cisplatin and induction of GFRA1 expression significantly suppressed apoptosis mediated by cisplatin in osteosarcoma cells. GFRA1 expression promotes autophagy by activating the SRC-AMPK signaling axis following cisplatin treatment, resulting in enhanced osteosarcoma cell survival. GFRA1-induced autophagy promoted tumor growth in mouse xenograft models, suggesting a novel function of GFRA1 in osteosarcoma chemoresistance.
A Systematic Study of RNAi Effects and dsRNA Stability in Tribolium castaneum and Acyrthosiphon pisum, Following Injection and Ingestion of Analogous dsRNAs
Authors: Cao Min , Gatehouse John A. , Fitches Elaine C.
Published: April 2018

RNA interference (RNAi) effects in insects are highly variable and may be largely dependent upon the stability of introduced double-stranded RNAs to digestion by nucleases. Here, we report a systematic comparison of RNAi effects in susceptible red flour beetle (Tribolium castaneum) and recalcitrant pea aphid (Acyrthosiphon pisum) following delivery of dsRNAs of identical length targeting expression of V-type ATPase subunit E (VTE) and inhibitor of apoptosis (IAP) genes. Injection and ingestion of VTE and IAP dsRNAs resulted in up to 100% mortality of T. castaneum larvae and sustained suppression (>80%) of transcript levels. In A. pisum, injection of VTE but not IAP dsRNA resulted in up to 65% mortality and transient suppression (ca. 40%) of VTE transcript levels. Feeding aphids on VTE dsRNA reduced growth and fecundity although no evidence for gene suppression was obtained. Rapid degradation of dsRNAs by aphid salivary, haemolymph and gut nucleases contrasted with stability in T. castaneum larvae where it appears that exo-nuclease activity is responsible for relatively slow digestion of dsRNAs. This is the first study to directly compare RNAi effects and dsRNA stability in receptive and refractory insect species and provides further evidence that dsRNA susceptibility to nucleases is a key factor in determining RNAi efficiency.
Teleosts Genomics: Progress and Prospects in Disease Prevention and Control
Authors: Munang’andu Hetron , Galindo-Villegas Jorge , David Lior
Published: April 2018

Genome wide studies based on conventional molecular tools and upcoming omics technologies are beginning to gain functional applications in the control and prevention of diseases in teleosts fish. Herein, we provide insights into current progress and prospects in the use genomics studies for the control and prevention of fish diseases. Metagenomics has emerged to be an important tool used to identify emerging infectious diseases for the timely design of rational disease control strategies, determining microbial compositions in different aquatic environments used for fish farming and the use of host microbiota to monitor the health status of fish. Expounding the use of antimicrobial peptides (AMPs) as therapeutic agents against different pathogens as well as elucidating their role in tissue regeneration is another vital aspect of genomics studies that had taken precedent in recent years. In vaccine development, prospects made include the identification of highly immunogenic proteins for use in recombinant vaccine designs as well as identifying gene signatures that correlate with protective immunity for use as benchmarks in optimizing vaccine efficacy. Progress in quantitative trait loci (QTL) mapping is beginning to yield considerable success in identifying resistant traits against some of the highly infectious diseases that have previously ravaged the aquaculture industry. Altogether, the synopsis put forth shows that genomics studies are beginning to yield positive contribution in the prevention and control of fish diseases in aquaculture.
Radiation-Induced Gene Expression Changes in High and Low Grade Breast Cancer Cell Types
Authors: Bravatà Valentina , Cava Claudia , Minafra Luigi , Cammarata Francesco Paolo , Russo Giorgio , Gilardi Maria Carla , Castiglioni Isabella , Forte Giusi Irma
Published: April 2018

Background: There is extensive scientific evidence that radiation therapy (RT) is a crucial treatment, either alone or in combination with other treatment modalities, for many types of cancer, including breast cancer (BC). BC is a heterogeneous disease at both clinical and molecular levels, presenting distinct subtypes linked to the hormone receptor (HR) status and associated with different clinical outcomes. The aim of this study was to assess the molecular changes induced by high doses of ionizing radiation (IR) on immortalized and primary BC cell lines grouped according to Human epidermal growth factor receptor (HER2), estrogen, and progesterone receptors, to study how HR status influences the radiation response. Our genomic approach using in vitro and ex-vivo models (e.g., primary cells) is a necessary first step for a translational study to describe the common driven radio-resistance features associated with HR status. This information will eventually allow clinicians to prescribe more personalized total doses or associated targeted therapies for specific tumor subtypes, thus enhancing cancer radio-sensitivity. Methods: Nontumorigenic (MCF10A) and BC (MCF7 and MDA-MB-231) immortalized cell lines, as well as healthy (HMEC) and BC (BCpc7 and BCpcEMT) primary cultures, were divided into low grade, high grade, and healthy groups according to their HR status. At 24 h post-treatment, the gene expression profiles induced by two doses of IR treatment with 9 and 23 Gy were analyzed by cDNA microarray technology to select and compare the differential gene and pathway expressions among the experimental groups. Results: We present a descriptive report of the substantial alterations in gene expression levels and pathways after IR treatment in both immortalized and primary cell cultures. Overall, the IR-induced gene expression profiles and pathways appear to be cell-line dependent. The data suggest that some specific gene and pathway signatures seem to be linked to HR status. Conclusions: Genomic biomarkers and gene-signatures of specific tumor subtypes, selected according to their HR status and molecular features, could facilitate personalized biological-driven RT treatment planning alone and in combination with targeted therapies.
Creativity as a Stepping Stone toward a Brighter Future
Authors: Kaufman James
Published: April 2018

If IQs continue to rise over generation, why has the world been unable to solve basic recurrent problems? This paper argues that creativity, which is overlooked in IQ tests and showing no signs of a similar increase, may be part of the reason of why the Flynn Effect has not led to a better world. Creativity’s predictive power for traditional positive outcomes, such as school or work performance, is significant but slight. However, there are other ways that creativity can help to make a better world. Two exemplar ways that are discussed in this paper are how creativity can (a) help people lead happier and more meaningful lives and (b) focus a spotlight on talented members of underrepresented groups who are overlooked by traditional measures. Both of these directions can lead to a world that is better equipped to solve larger issues.
Geometrical Structures and Electronic Properties of Ga6 and Ga5X (X = B, C, N, O, F, Al, Si, P, S, Cl) Clusters
Authors: Hu Yanfei , Ji Guangfu , Yao Yachuan , Yuan Jiaonan , Xu Weisen
Published: April 2018

Based on the unbiased CALYPSO (Crystal structure Analysis by Particle Swarm Optimization) structure searching method in combination with density functional theory (DFT), the geometrical structures and electronic properties are investigated theoretically for Ga6 and Ga5X (X = B, C, N, O, F, Al, Si, P, S, Cl) clusters. The PBE0 exchange-correlation functional and the 6-311G(d) basis set is carried out to determine global minima on potential energy surfaces. The relative stabilities of the clusters are examined by the binding energies and substitution reaction. Following the predictions of the Jellium model, the Ga5B cluster with the 18 valence electrons is the most stable structure. At last, with the obtained lowest energy structures, some physical properties such as electrons transfer, molecular orbitals, and total and partial densities of states are discussed, respectively.