bluuebunny/crossref_metadata_2025 huggingface dataset:
Allergies to Allergens from Cats and Dogs: A Review and Update on Sources, Pathogenesis, and Strategies
Authors: An Wei , Li Ting , Tian Xinya , Fu Xiaoxin , Li Chunxiao , Wang Zhenlong , Wang Jinquan , Wang Xiumin
Published: September 2024

Inhalation allergies caused by cats and dogs can lead to a range of discomforting symptoms, such as rhinitis and asthma, in humans. With the increasing popularity of and care provided to these companion animals, the allergens they produce pose a growing threat to susceptible patients’ health. Allergens from cats and dogs have emerged as significant risk factors for triggering asthma and allergic rhinitis worldwide; however, there remains a lack of systematic measures aimed at assisting individuals in recognizing and preventing allergies caused by these animals. This review provides comprehensive insights into the classification of cat and dog allergens, along with their pathogenic mechanisms. This study also discusses implementation strategies for prevention and control measures, including physical methods, gene-editing technology, and immunological approaches, as well as potential strategies for enhancing allergen immunotherapy combined with immunoinformatics. Finally, it presents future prospects for the prevention and treatment of human allergies caused by cats and dogs. This review will improve knowledge regarding allergies to cats and dogs while providing insights into potential targets for the development of next-generation treatments.
Immunomodulatory Effect of Adipose Stem Cell-Derived Extra-Cellular Vesicles on Cytokine Expression and Regulatory T Cells in Patients with Asthma
Authors: Jung Jae Hoon , Kang Shin Ae , Park Ji-Hwan , Kim Sung-Dong , Yu Hak Sun , Mun Sue Jean , Cho Kyu-Sup
Published: September 2024

Although mesenchymal stem cell (MSC)-derived extracellular vesicles (EVs) are as effective as MSCs in the suppression of allergic airway inflammation, few studies have evaluated the immunomodulatory capacity of MSC-derived EVs in patients with asthma. Thus, we assessed the effects of adipose stem cell (ASC)-derived EVs on cytokine expression and regulatory T cells (Tregs) in peripheral blood mononuclear cells (PBMCs) of asthmatic patients. PBMCs (1 × 106 cells/mL) were isolated from asthmatic patient and healthy controls and co-cultured with 1 μg/mL of ASC-derived EVs. Th (T helper) 1-, Th2-, and Treg-related cytokine expression, fluorescence-activated cell sorting analysis of CD4+CD25+FOXP3+ T cells, and co-stimulatory molecules were analyzed before and after ASC-derived EV treatment. The expression levels of IL-4 and costimulatory molecules such as CD83 and CD86 were significantly higher in PBMCs of asthmatic patients than in control PBMCs. However, ASC-derived EV treatment significantly decreased the levels of interleukin (IL)-4 and co-stimulatory molecules such as CD83 and CD86 in the phytohemagglutinin (PHA)-stimulated PBMC of asthmatic patients. Furthermore, ASC-derived EVs remarkably increased the transforming growth factor-β (TGF-β) levels and expression of Tregs in the PBMC of asthmatic patients. ASC-derived EVs induce Treg expansion and have immunomodulatory effects by downregulating IL-4 and upregulating TGF-β in PBMCs of asthmatic patients.
Preliminary Investigation of Potential Early Biomarkers for Gestational Diabetes Mellitus: Insights from PTRPG and IGKV2D-28 Expression Analysis
Authors: Payot Mariejim Diane , Villavieja Adrian , Pineda-Cortel Maria Ruth
Published: September 2024

Gestational diabetes mellitus (GDM) poses significant health risks to both mothers and infants, emphasizing the need for early detection strategies to mitigate its impact. However, the existing diagnostic methods, particularly the oral glucose tolerance test (OGTT) administered in the second or third trimester, show limitations in the detection of GDM during its early stages. This study aimed to explore the potential of the genes Protein Tyrosine Phosphatase Receptor-type Gamma (PTPRG) and Immunoglobulin Kappa Variable 2D-28 (IGKV2D-28) as early indicators for GDM among Filipino pregnant women. Utilizing reverse transcription–quantitative polymerase chain reaction (RT-qPCR), the gene expressions were analyzed in first-trimester blood samples obtained from 24 GDM and 36 non-GDM patients. The diagnostic performance of PTPRG and IGKV2D-28 was analyzed and evaluated using receiver operating characteristic (ROC) curves. The findings revealed elevated expression levels of PTPRG and IGKV2D-28 within the GDM cohort. Remarkably, PTPRG exhibited a sensitivity of 83%, while IGKV2D-28 demonstrated a specificity of 94% at determined cut-off values. Combining both genes yielded an improved but limited diagnostic accuracy with an area under the curve (AUC) of 0.63. This preliminary investigation of PTPRG and IGKV2D-28 sheds light on novel avenues for early GDM detection. While these findings are promising, further validation studies in larger cohorts are necessary to confirm these results and explore additional biomarkers to enhance diagnostic precision in GDM pregnancies and, ultimately, to improve maternal and fetal outcomes.
Untargeted Metabolomics Approach for the Discovery of Salinity-Related Alkaloids in a Stony Coral-Derived Fungus Aspergillus terreus
Authors: Liu Yayue , Wang Li , Feng Yunkai , Liao Qingnan , Lei Xiaoling , Hu Xueqiong , Zhou Longjian , Zhang Yi
Published: September 2024

As a part of the important species that form coral reef ecosystems, stony corals have become a potential source of pharmacologically active lead compounds for an increasing number of compounds with novel chemical structures and strong biological activity. In this study, the secondary metabolites and biological activities are reported for Aspergillus terreus C21-1, an epiphytic fungus acquired from Porites pukoensis collected from Xuwen Coral Reef Nature Reserve, China. This strain was cultured in potato dextrose broth (PDB) media and rice media with different salinities based on the OSMAC strategy. The mycelial morphology and high-performance thin layer chromatographic (HPTLC) fingerprints of the fermentation extracts together with bioautography were recorded. Furthermore, an untargeted metabolomics study was performed using principal component analysis (PCA), orthogonal projection to latent structure discriminant analysis (O-PLSDA), and feature-based molecular networking (FBMN) to analyze their secondary metabolite variations. The comprehensive results revealed that the metabolite expression in A. terreus C21-1 differed significantly between liquid and solid media. The metabolites produced in liquid medium were more diverse but less numerous compared to those in solid medium. Meanwhile, the mycelial morphology underwent significant changes with increasing salinity under PDB cultivation conditions, especially in PDB with 10% salinity. Untargeted metabolomics revealed significant differences between PDB with 10% salinity and other media, as well as between liquid and solid media. FBMN analysis indicated that alkaloids, which might be produced under high salt stress, contributed largely to the differences. The biological activities results showed that six groups of crude extracts exhibited acetylcholinesterase (AChE) inhibitory activities, along with 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging and antibacterial activities. The results of this study showed that the increase in salinity favored the production of unique alkaloid compounds by A. terreus C21-1.
Bioinformatic-Experimental Screening Uncovers Multiple Targets for Increase of MHC-I Expression through Activating the Interferon Response in Breast Cancer
Authors: Li Xin , Ruan Zilun , Yang Shuzhen , Yang Qing , Li Jinpeng , Hu Mingming
Published: September 2024

Expression of major histocompatibility complex I (MHC-I) on tumor cells is extremely important for the antitumor immune response for its essential role in activating various immune cells, including tumor-specific CD8+ T cells. Cancers of lower MHC-I expression commonly exhibit less immune cell infiltration and worse prognosis in clinic. In this study, we conducted bioinformatic-experimental screening to identify potential gene targets to enhance MHC-I expression in breast cancer (BRCA). Through a combination of MHC-I scoring, gene expression correlation analysis, survival prognostication, and Cibersort tumor-infiltrated lymphocytes (TILs) scoring, we identify 144 genes negatively correlated with both MHC-I expression and TILs in breast cancer. Furthermore, we verified partially according to KEGG functional enrichment or gene-dependency analysis and figured out multiple genes, including PIP5K1A, NCKAP1, CYFIP1, DIS3, TBP, and EXOC1, as effective gene targets for increasing MHC-I expression in breast cancer. Mechanistically, knockout of each of these genes activated the intrinsic interferon response in breast cancer cells, which not only promoted MHC-I expression but also caused immunogenic cell death of breast cancer. Finally, the scRNA-seq confirmed the negative correlation of PIP5K1A et al. with TILs in breast cancer patients. Collectively, we identified multiple gene targets for an increase in MHC-I expression in breast cancer in this study.
Transcriptomic Approach Reveals Contrasting Patterns of Differential Gene Expression during Tannin Biodegredation by Aspergillus tubingensis in Liquid and Solid Cultures
Authors: Zeng Xiaona , Song Jiabei , Tang Shengqiu , Dong Xiaoying , Chen Sheng , Kong Jie , Chen Liyi , Li Yajuan , Shao Guanming , Wong Yung-Hou , Xie Qingmei
Published: September 2024

Tannins, one of the most common anti-nutritional factors in feed, can be effectively degraded by various enzymes secreted by Aspergillus tubingensis (A. tubingensis). The cultivation method of fungi significantly impacts gene expression, which influences the production of enzymes and metabolites. In this study, we analyzed the tannin biodegredation efficiency and the transcriptomic responses of A. tubingensis in liquid and solid cultures with tannin added. The observed morphology of A. tubingensis resembled typical fungal hyphae of mycelium submerged and grown in liquid cultures, while mainly spore clusters were observed in solid cultures. Furthermore, the tannin biodegredation efficiency and protein secretion of A. tubingensis in liquid cultures were significantly higher than in solid cultures. Additionally, 54.6% of the 11,248 differentially expressed genes were upregulated in liquid cultures, including AtWU_03490 (encoding ABC multidrug transporter), AtWU_03807 (ribonuclease III), AtWU_10270 (peptidyl-tRNA hydrolase), and AtWU_00075 (arabinogalactan endo-1,4-beta-galactosidase). Functional and gene ontology enrichment analyses indicated upregulation in processes including oxidation reduction, drug metabolism, and monocarboxylic acid metabolism. Overall, this study provides insight into the transcriptomic response to tannin biodegradation by A. tubingensis in different cultures and reveals that liquid cultures induce greater transcriptomic variability compared to solid cultures.
Genome-Wide Association Study on Body Conformation Traits in Xinjiang Brown Cattle
Authors: Zhang Menghua , Wang Yachun , Chen Qiuming , Wang Dan , Zhang Xiaoxue , Huang Xixia , Xu Lei
Published: September 2024

Body conformation traits are linked to the health, longevity, reproductivity, and production performance of cattle. These traits are also crucial for herd selection and developing new breeds. This study utilized pedigree information and phenotypic (1185 records) and genomic (The resequencing of 496 Xinjiang Brown cattle generated approximately 74.9 billion reads.) data of Xinjiang Brown cattle to estimate the genetic parameters, perform factor analysis, and conduct a genome-wide association study (GWAS) for these traits. Our results indicated that most traits exhibit moderate to high heritability. The principal factors, which explained 59.12% of the total variance, effectively represented body frame, muscularity, rump, feet and legs, and mammary system traits. Their heritability estimates range from 0.17 to 0.73, with genetic correlations ranging from −0.53 to 0.33. The GWAS identified 102 significant SNPs associated with 12 body conformation traits. A few of the SNPs were located near previously reported genes and quantitative trait loci (QTLs), while others were novel. The key candidate genes such as LCORL, NCAPG, and FAM184B were annotated within 500 Kb upstream and downstream of the significant SNPs. Therefore, factor analysis can be used to simplify multidimensional conformation traits into new variables, thus reducing the computational burden. The identified candidate genes from GWAS can be incorporated into the genomic selection of Xinjiang Brown cattle, enhancing the reliability of breeding programs.
Gene Expression Aberrations in Alcohol-Associated Hepatocellular Carcinoma
Authors: Petrović Andreja , Štancl Paula , Gršković Paula , Hančić Suzana , Karlić Rosa , Gašparov Slavko , Korać Petra
Published: September 2024

Hepatocellular carcinoma (HCC) is the most prevalent primary liver cancer, ranking as the sixth most common cancer worldwide and the fourth leading cause of cancer-related deaths. Most HCC cases originate from cirrhotic livers, typically due to chronic liver diseases, such as hepatitis B (HBV) and hepatitis C (HCV) infections, and alcoholism. HCC cells often harbor numerous somatic mutations that are implicated in HCC development, but epigenetic factors, such as miRNA interference, can also affect HCC initiation and progress. miRNA-221 has been explored as a factor affecting HCC development in HCC of viral etiology, but little is known about its effects on gene expression in alcohol-associated HCC. This study aimed to explore potentially similar gene expression aberrations underlying viral and alcohol-induced HCC. We analyzed available transcriptome data from non-tumor hepatocytes and viral-induced HCC tissues. The most notable differences in gene expression associated with miRNA-221 between non-tumor hepatocytes and viral-induced HCC involved NTF-3 and MYBL1 genes. To assess these data in alcohol-induced HCC, we examined 111 tissue samples: tumor tissue and cirrhotic tissue samples from 37 HCC patients and 37 samples from non-tumor liver tissue using RT-Q PCR. We found no significant difference in NTF-3 expression, but MYBL1 expression was significantly lower in HCC tissue compared to non-tumor hepatocytes and cirrhotic tissue. Our findings highlight the importance of the MYBL1 gene in HCC development and emphasize the need for diverse approaches in evaluating tumor mechanisms.
Preparation, Characterization, and Wound Healing Promotion of Hydrogels Containing Glucosyloxybenzyl 2-Isobutylmalates Extract from Bletilla striata (Thunb.) Reichb.f.
Authors: Ran Fei , Mu Kailang , Liu Gang , Liu Yuchen , Pang Yuxin , Feng Guo , Zhou Lingli , Peng Leqiang
Published: September 2024

Plant-derived medicinal materials have significant potential and promising applications in wound healing and skin regeneration. This study aims to develop a plant-based extract hydrogel from Bletilla striata (Thunb.Reichb.f.), specifically a glucosyloxybenzyl 2-isobutylmalates extract (B), and characterize its potential effects on wound healing. We synthesized the hydrogel using carbomer (C), glycerol (G), and triethanolamine (T) as the matrix, incorporating B into the hydrogel base, and evaluated its physical and chemical properties. In vitro tests assessed the biocompatibility of the glucosyloxybenzyl 2-isobutylmalates-carbomer-glycerol-triethanolamine (B-CGT) hydrogel and its effects on cell proliferation, migration, and adhesion. Animal model experiments evaluated its potential to promote wound healing. The results showed that the prepared B-CGT hydrogel possessed a good three-dimensional network structure and stability, demonstrating significant free radical scavenging capacity in antioxidant tests. In cell experiments, the B-CGT hydrogel exhibited no potential cytotoxicity and showed good hemocompatibility and promotion of cell proliferation. Animal experiments indicated that wounds treated with the B-CGT hydrogel healed significantly faster, with improved formation of new epithelial tissue and collagen. This study suggests that the developed B-CGT hydrogel is a promising candidate for wound dressings, with excellent physicochemical properties and controlled drug release capabilities, effectively promoting the wound healing process.
Bioceramics Enhance the Anti-Tumor Activity of Immune Cells in Adoptive Immunotherapy
Authors: Nose Masato , Nitta Aiko , Zheng Yundi , Kizukuri Rihoko , Nagao Yuki , Nagai Shigenori , Aizawa Mamoru
Published: September 2024

Recent research has focused on immunotherapy with no side effects as an innovative medical treatment for cancer. However, typical drugs for immunotherapy are very expensive. Here, we propose the use of immunoceramics that activate immune cells by contact with their surface. Previous studies demonstrated that polymers, including the phenylboronic acid group, could activate lymphocytes. This activation may be due to the interaction between the sugar chains in cells and the OH group in B(OH)3 formed via the dissociation of the BO2 group. We have clarified that boron-containing apatite (BAp) activated lymphocytes in vitro. In this study, we fabricated the ceramic surfaces using the CaO-P2O5-SiO2-B2O3 system (CPSB ceramics) containing BAp as a main crystalline phase. The results of the in vitro evaluation indicated that killer T cells in splenocytes cocultured with the CPSB ceramics were more numerous than in splenocytes cocultured on a control surface. The results of the in vivo evaluation indicated that the CPSB ceramics significantly inhibited tumor growth when CD8-positive T cells were cultured on individual ceramics and subsequently injected into tumor-bearing mice. The present CPSB ceramics are expected to be a valuable biomaterial for immunotherapy.
Morphological and Taxonomic Analysis of the Quercus faginea and Quercus canariensis (Fagaceae) Complexes in Algeria
Authors: Bouandas Ameur , Belhoucine-Guezouli Latifa , Oliva Francesc , Gaouar Semir Bechir Suheil , Bendjebbar Khedidja , Vázquez Pardo Francisco M. , Pujade-Villar Juli
Published: September 2024

The valid deciduous Quercus L. species from North Africa have been largely discussed by many authors. The current species remain yet uncertain. In this study, we compare several populations of presumably Q. canariensis Willd. and Q. faginea Lam. from Algeria with pure populations of these species from the northeast of the Iberian Peninsula. Different morphological characters from leaves have been analyzed. Principal components analysis and a canonical analysis of principal coordinates have been used to observe the relationship between samples, groups and the seven quantitative variables. Distances among centroids have been reported and a SIMPER procedure has also been executed to better explain the different variability within and between groups. PERMANOVA has been applied to test for significant differences between the groups. For the trichomes study, ANOVA models have been used. From our analysis, we conclude that in Algeria, we have a single Q. canariensis Willd. population, different from the Iberian population we examined. It probably corresponds morphologically to Q. mirbeckii Durieu, currently considered a synonymy of Q. canariensis Willd., and for the “Q. faginea” group we have two Algerian populations: Q. faginea Lam. subsp. faginea, found in the northeast Iberian Peninsula, and Q. tlemcenensis (A.DC.) Maire and Weiller ex Greuter and Burdet. Previous results from other authors have also been discussed.
Gender Bias in Text-to-Image Generative Artificial Intelligence When Representing Cardiologists
Authors: Currie Geoffrey , Chandra Christina , Kiat Hosen
Published: September 2024

Introduction: While the global medical graduate and student population is approximately 50% female, only 13–15% of cardiologists and 20–27% of training fellows in cardiology are female. The potentially transformative use of text-to-image generative artificial intelligence (AI) could improve promotions and professional perceptions. In particular, DALL-E 3 offers a useful tool for promotion and education, but it could reinforce gender and ethnicity biases. Method: Responding to pre-specified prompts, DALL-E 3 via GPT-4 generated a series of individual and group images of cardiologists. Overall, 44 images were produced, including 32 images that contained individual characters and 12 group images that contained between 7 and 17 characters. All images were independently analysed by three reviewers for the characters’ apparent genders, ages, and skin tones. Results: Among all images combined, 86% (N = 123) of cardiologists were depicted as male. A light skin tone was observed in 93% (N = 133) of cardiologists. The gender distribution was not statistically different from that of actual Australian workforce data (p = 0.7342), but this represents a DALL-E 3 gender bias and the under-representation of females in the cardiology workforce. Conclusions: Gender bias associated with text-to-image generative AI when using DALL-E 3 among cardiologists limits its usefulness for promotion and education in addressing the workforce gender disparities.
Building Digital Trust and Rapport in the Tourism Industry: A Bibliometric Analysis and Detailed Overview
Authors: Elshaer Ibrahim A. , Alyahya Mansour , Azazz Alaa M. S. , Ali Mohamed Ali Shabeeb , Fathy Eslam Ahmed , Fouad Amr Mohamed , Soliman Shimaa Abo Elsoad Mohamed , Fayyad Sameh
Published: September 2024

In the rapidly changing environment of the tourism business, building digital trust and rapport has appeared as a critical factor for sustaining profitable customer relationships and confirming long-term business survival. This study aims to provide a comprehensive overview of the research landscape with respect to digital trust and rapport establishment within the tourism context using techniques for bibliometric analysis in conjunction with visualization. This research paper explores publication trends, identifies key journals and authors, and determines the most productive countries, academic institutions, and primary research keywords. It involves analyzing publications from the Scopus database focusing on the duration between 2014 and 2023. The findings show that the digital trust and rapport-building literature expands its importance within the tourism industry due to factors such as competition pressure; customers’ high expectations; the incorporation of technology; and, most recently, the occurrence of pandemics like the COVID-19 pandemic. The US, in particular, can be identified as the most productive country in this research area. The International Journal of Contemporary Hospitality Management and the International Journal of Hospitality Management appear to be popular scientific publications on this topic. They present the following main research themes: customer engagement, employee conduct, and technology in the construction of trust. This study also underscores the practical role of the findings in successful endeavors within the field, detailing how cultural sensitivity, technological incorporation, and accessibility in service provision would contribute to building digital trust with international customers. Potential future research directions are proposed to advance the understanding of culture, technology, the effects of crises, and the application of other research methods.
Towards a QBLM-Based Qualification-Management Methodology Supporting Human-Resource Management and Development
Authors: Vogler Adrian , Vu Binh , Then Matthias , Hemmje Matthias
Published: September 2024

This position paper presents a novel perspective on addressing the challenges of digital transformation in higher education through the development of a qualification-based learning model (QBLM) qualification management methodology. It argues that the rapid pace of technological advancement and the resulting need for continuous upskilling and reskilling necessitate a more dynamic and adaptive approach to human-resource management and development. The paper posits that by extending QBLM through the integration of artificial intelligence (AI) and machine learning (ML), a more effective system for analyzing competence requirements and designing personalized learning pathways can be created. The paper proposes a three-fold approach: (1) developing the FPHR ontology to support semantic annotation of HR qualifications in higher-education institutions (HEIs), (2) integrating this ontology into QBLM to ensure the machine-readability of qualifications, and (3) modeling a knowledge-based production process for HRs in skills-based learning. This paper outlines the current state of the art, presents conceptual models, and describes planned proof-of-concept implementations and evaluations. It contends that this approach will significantly enhance the effectiveness of human-resource development in the rapidly evolving digital knowledge society. By presenting this position, the paper aims to stimulate discussion and collaboration within the academic community on innovative approaches to qualification management in higher education. The work addresses critical issues arising from technological development and offers a forward-thinking solution to bridge the gap between current and future skill requirements in industry and academia.
Additive Manufacturing, Numerical and Experimental Analyses for Pentamode Metamaterials
Authors: Lymperopoulos Panagiotis N. , Theotokoglou Efstathios E. , Dragatogiannis Dimitrios , Karalekas Dimitrios , Matsika-Klossa Constantina
Published: September 2024

Pentamodes are lattice structures composed of beams. Their main property is the low ratio of the shear to bulk modulus, making them suitable for aerospace, antiseismic, and bioengineering applications. At first, in our study, pentamode structures were fabricated using three-dimensional printing and were tested in a laboratory. Then, computational analyses of bulk strength have been performed. In addition, several preliminary computational analyses have been considered, comparing different pentamodes’ dimensions and topologies in order to understand their behaviour under different loading conditions. Experimental results have been compared with the numerical results in order to validate the forces applied to the lattice structures. Our new contribution is that for the first time, the experimental and numerical results are investigated up to the failure of the specimens, the effective Young’s modulus has been calculated for different pentamode lattice structures, and our results are also compared with analytical equations.
Attraction of Sweet Potato Whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae), and Two Generalist Predators to Green Leaf Volatile Compounds
Authors: Gaffke Alexander M. , Miller Neil W. , Sharma Anamika , Allan Sandra A.
Published: September 2024

Traditionally, olfaction was thought to play a minor role in the behavioral ecology of the sweet potato whitefly, Bemisia tabaci (Gennadius). However, recent research is uncovering significant potential for whitefly management based on olfaction. Incorporating chemical attractants with standard whitefly management programs could significantly improve control. The integration of attractants with biological control is exceptionally promising. Therefore, the behavioral response of B. tabaci and two generalist predators to the green leaf volatiles (E)-2-hexenal, (Z)-3-hexenal, (Z)-3-hexenyl acetate, and (Z)-3-hexe-1-ol were investigated in Y-tube olfactometers. Three of the four green leaf volatiles resulted in the attraction of female B. tabaci. Blend optimization indicated a two-chemical blend to be the most attractive blend for female B. tabaci. In addition, this blend was attractive to female Macrolophus praeclarus (Distant) but did not elicit a behavioral response from either male or female Delphastus catalinae (Horn). The two-chemical blend of green leaf volatiles could be further developed as a lure to attract B. tabaci and its predator, M. praeclarus.
Limited Variation in Codon Usage across Mitochondrial Genomes of Non-Biting Midges (Diptera: Chironomidae)
Authors: Lei Teng , Zheng Xiaojun , Song Chao , Jin Haobo , Chen Lingjun , Qi Xin
Published: September 2024

The codon usage patterns of mitochondrial genomes offer insights into the evolutionary and phylogenetic studies of species. Codon usage analysis has been conducted in a few Chironomidae species, and the codon usage patterns in other species remain ambiguous. We aim to reveal the codon usage differences in the mitochondrial genomes across this family. We sequenced the first mitochondrial genome of the genus Conchapelopia and the third mitochondrial genome of the subfamily Tanypodinae. Then, we analyzed its relative synonymous codon usage and effective number of codons with registered mitochondrial genomes from 28 other genera. The results indicated that there was limited variation in codon usage across five subfamilies, Chironominae, Orthocladiinae, Diamesinae, Prodiamesinae and Tanypodinae. While Parochlus steinenii from Podonominae presented a weaker codon bias, P. steinenii possessed the most genes experiencing natural selection. Additionally, ND1, ND2 and ND3 were found to be the most frequently selected genes across all species. Our findings contribute to further understanding the evolutionary and phylogenetic relationships of Chironomidae.
Genetic Attributes and Conservation of an Endangered Giant Water Bug Species, Diplonychus esakii Miyamoto and Lee, 1966 (Hemiptera: Belostomatidae)
Authors: Kim Seon Yi , Lim Changseob , Kang Ji Hyoun , Bae Yeon Jae
Published: September 2024

Diplonychus esakii, a water bug from the family Belostomatidae, plays an important role in freshwater ecosystems as one of the top predators. In this study, we investigated the genetic diversity and population structure of D. esakii by analyzing 318 specimens across 27 sites in South Korea. We found that the populations of D. esakii possess 11 haplotypes with a haplotype diversity of 0.623. This represents a relatively low level of genetic diversity compared to other known belostomatids and endangered species. AMOVA and FST analyses revealed significant genetic differentiation among populations, with most populations harboring only 1–2 haplotypes, suggesting restricted gene flow between populations and a low level of genetic diversity. This low genetic diversity and limited gene flow suggest a potential vulnerability to environmental changes and an increased risk of extinction, indicating that D. esakii should be designated as a protected species in South Korea as part of future conservation efforts. Based on the results of this study, Upo Wetland, which maintains relatively high levels of genetic diversity and Jeju Island, which, despite its lower genetic diversity compared to the mainland, does not share haplotypes with other regions, should be considered key conservation units for this species. This study highlights the importance of incorporating genetic information into conservation status assessments under the Red List Categories and Criteria and also emphasizes the need to evaluate this species on the Korean Red List. The data provided here will serve as essential baseline information and valuable resources for the development of effective conservation strategies.
Industrial IoT-Based Energy Monitoring System: Using Data Processing at Edge
Authors: Mirani Akseer Ali , Awasthi Anshul , O’Mahony Niall , Walsh Joseph
Published: September 2024

Edge-assisted IoT technologies combined with conventional industrial processes help evolve diverse applications under the Industrial IoT (IIoT) and Industry 4.0 era by bringing cloud computing technologies near the hardware. The resulting innovations offer intelligent management of the industrial ecosystems, focusing on increasing productivity and reducing running costs by processing massive data locally. In this research, we design, develop, and implement an IIoT and edge-based system to monitor the energy consumption of a factory floor’s stationary and mobile assets using wireless and wired energy meters. Once the edge receives the meter’s data, it stores the information in the database server, followed by the data processing method to find nine additional analytical parameters. The edge also provides a master user interface (UI) for comparative analysis and individual UI for in-depth energy usage insights, followed by activity and inactivity alarms and daily reporting features via email. Moreover, the edge uses a data-filtering technique to send a single wireless meter’s data to the cloud for remote energy and alarm monitoring per project scope. Based on the evaluation, the edge server efficiently processes the data with an average CPU utilization of up to 5.58% while avoiding measurement errors due to random power failures throughout the day.
Adolescent Pregnancy: A Comparative Insight into the Prevalence and Risks of Obstetric Complications in a Polish Cohort
Authors: Staniczek Jakub , Manasar-Dyrbuś Maisa , Stojko Rafał , Jendyk Cecylia , Sadłocha Marcin , Winkowska Ewa , Orszulak Dominika , Niziński Kacper , Skowronek Kaja , Toczek Jakub , Matonóg Aleksandra , Wilk Katarzyna , Zięba-Domalik Maja , Sieroszewska Diana , Sieroszewski Aleksander , Starczewska Joanna , Sowa-Sanchez Daria , Jurecki Jakub , Troszka Jonasz , Stojko Szymon , Drosdzol-Cop Agnieszka
Published: September 2024

Background: Adolescent pregnancy is associated with increased risk of both maternal and neonatal complications. Common maternal complications include anemia, hypertensive disorders, and a higher incidence of infections, including Group B Streptococcus (GBS). Additionally, adolescents are at increased risk for gestational diabetes and postpartum hemorrhage. Neonatal complications often involve low birth weight, prematurity, and an increased likelihood of neonatal intensive care unit (NICU) admission. Objectives: This study aims to assess and compare the prevalence of obstetric complications between adolescent and older pregnant women. Methods: This retrospective study investigates obstetric outcomes in adolescent pregnancies, analyzing data collected from 1 January 2016 to 30 June 2024. This study included 902 participants, of whom 224 were adolescents. The variables were categorized into maternal, birth, and neonatal characteristics. Results: Adolescent patients demonstrated a significantly higher prevalence of Group B Streptococcus (GBS) infection, affecting 25.89% of this group. Adolescent patients reported 17.86% nicotine use during pregnancy, a rate significantly higher than that of older age groups (p < 0.001). Additionally, adolescent pregnancies were associated with the highest mean blood loss during delivery, averaging 500 mL during vaginal birth and 1050 mL during cesarean section, leading to a higher incidence of blood transfusions (3.13%, p = 0.021). Newborns from adolescent pregnancies had the lowest mean birth weight (3199 g) and length (53.6 cm). Neonatal complications were more frequent in this group, affecting 20.09% of newborns, with a significantly higher rate of admission to intensive care units (2.68%, p = 0.008). These findings underscore the need for targeted interventions and more proactive management strategies to address the specific challenges faced by this population.
Antidiabetic Treatment and Prevention of Ischemic Stroke: A Systematic Review
Authors: Prentza Vasiliki , Pavlidis George , Ikonomidis Ignatios , Pililis Sotirios , Lampsas Stamatios , Kountouri Aikaterini , Pliouta Loukia , Korakas Emmanouil , Thymis John , Palaiodimou Lina , Tsegka Aikaterini , Markakis Konstantinos , Halvatsiotis Panagiotis , Tsivgoulis Georgios , Lambadiari Vaia
Published: September 2024

Background: Diabetes mellitus (DM) is a prevalent disease in the general population and also a well-established risk factor for the development of ischemic stroke. Patients who have been diagnosed with diabetes have a 20% higher risk for developing ischemic stroke in comparison to non-diabetic individuals. The aim of the current systematic review is to provide the latest evidence regarding the association between antidiabetic treatment and the prevention of ischemic stroke. Methods: A comprehensive search in scientific literature databases PUBMED, COCHRANE, and SCOPUS was conducted. The studies that were deemed as eligible for this review were those that examined the clinical benefits of therapeutic strategies in terms of preventing ischemic strokes. Results: A total of 32 studies met the established selection criteria. The included studies showed that pioglitazone treatment significantly reduced the risk for recurrent stroke in patients with DM. Furthermore, in the context of primary prevention, the improvement in glycemic control after treatment with the glucagon-like peptide-1 receptor agonists (GLP-1RA) semaglutide and dulaglutide was associated with a reduction in the risk of ischemic stroke in diabetic subjects. Metformin monotherapy may reduce stroke risk, while dipeptidyl peptidase 4 inhibitors, sodium-glucose co-transporter 2 inhibitors, and insulin do not seem to affect the incidence of stroke. Conclusions: The findings of the present systematic review suggest that pioglitazone and GLP-1RA may decrease the risk of stroke. Further studies are needed to provide additional data regarding the preventive effect of novel antidiabetic drugs, such as dual glucose-dependent insulinotropic polypeptide/GLP-1RA agents, on stroke.
Impact of Reactive Balance Training on a Perturbation Treadmill on Physical Performance in Geriatric Patients:Results of a Single-Center, Assessor Blinded Randomized Controlled Trial
Authors: Petrovic Alexander , Wirth Rainer , Klimek Christiane , Lueg Gero , Daubert Diana , Giehl Chantal , Trampisch Ulrike Sonja
Published: September 2024

Background/Objectives: Falls and related injuries are a frequent and serious health problem in older persons. Among the various strategies, different forms of active physical training, in particular, have demonstrated success in reducing fall risk. A task-specific training approach is perturbation-based training of reactive balance. Performing this training modality on a perturbation treadmill, secured with a safety harness, is an innovative new approach facilitating task-specific training with unannounced perturbations in a safe environment. The aim of this study was to investigate the feasibility and effectiveness of this specific training in multimorbid older hospitalized patients with prefrailty and frailty. Methods: The trial was conducted as a prospective single-center, assessor-blinded randomized controlled trial. A total of 127 acute-care geriatric hospitalized patients were enrolled in a program either involving a minimum of 60 min perturbation-based treadmill training or treadmill training without perturbations on the identical device and for a comparable training period. Results: Participants were 81 ± 6 years old (64% female) with a baseline FRAIL Scale, SPPB, and MoCA scores of 3.5 ± 1.6, 8.3 ± 2.6, and 21 ± 5 points, respectively. The training was performed on six occasions with an average total training period of 89 min during a mean hospital stay of 17 ± 3 days. Between the baseline and up to 2 days after the last training, the Short Physical Performance Battery score, which was considered the primary endpoint, improved by 1.4 ± 2.1 points in the intervention group compared to 0.5 ± 1.7 in the control group, with a 0.9-point difference between the groups (p < 0.001). Conclusions: a relatively short training period of approximately 90 min on a perturbation treadmill led to a significant and clinically meaningful increase in the physical performance of frail and prefrail hospitalized geriatric patients. However, its effectiveness in reducing fall risk is yet to be proven in this population.
Optical Coherence Tomography in Myocardial Infarction Management: Enhancing Precision in Percutaneous Coronary Intervention
Authors: Buonpane Angela , Trimarchi Giancarlo , Ciardetti Marco , Coceani Michele Alessandro , Alagna Giulia , Benedetti Giovanni , Berti Sergio , Andò Giuseppe , Burzotta Francesco , De Caterina Alberto Ranieri
Published: September 2024

In acute myocardial infarction (AMI), the urgency of coronary revascularization through percutaneous coronary intervention (PCI) is paramount, offering notable advantages over pharmacologic treatment. However, the persistent risk of adverse events, including recurrent AMI and heart failure post-revascularization, underscores the necessity for enhanced strategies in managing coronary artery disease. Traditional angiography, while widely employed, presents significant limitations by providing only two-dimensional representations of complex three-dimensional vascular structures, hampering the accurate assessment of plaque characteristics and stenosis severity. Intravascular imaging, specifically optical coherence tomography (OCT), significantly addresses these limitations with superior spatial resolution compared to intravascular ultrasound (IVUS). Within the context of AMI, OCT serves dual purposes: as a diagnostic tool to accurately identify culprit lesions in ambiguous cases and as a guide for optimizing PCI procedures. Its capacity to differentiate between various mechanisms of acute coronary syndrome, such as plaque rupture and spontaneous coronary dissection, enhances its diagnostic potential. Furthermore, OCT facilitates precise lesion preparation, optimal stent sizing, and confirms stent deployment efficacy. Recent meta-analyses indicate that OCT-guided PCI markedly improves safety and efficacy in revascularization, subsequently decreasing the risks of mortality and complications. This review emphasizes the critical role of OCT in refining patient-specific therapeutic approaches, aligning with the principles of precision medicine to enhance clinical outcomes for individuals experiencing AMI.
The Impact of Telemedicine during the COVID-19 Pandemic on Diabetes Management and Pregnancy Outcomes in Women with Gestational Diabetes Mellitus (GDM)
Authors: Cichocka Edyta , Gumprecht Janusz
Published: September 2024

Background/Objectives: Gestational diabetes mellitus (GDM) can lead to various complications for both the mother and the child. Many factors influence the onset of the disease including GDM in a previous pregnancy, overweight and obesity, as well as the increasing age of women who become pregnant. The aim of this study was to assess the impact of telemedicine during the COVID-19 pandemic on diabetes management and pregnancy outcomes in women with gestational diabetes mellitus (GDM). Methods: A retrospective analysis was conducted. We compared two groups of GDM women from the pandemic and pre-pandemic periods in terms of pregnancy course and obstetric outcomes. Results: It was observed that women with GDM in the pandemic group were significantly more often overweight and significantly less often obese before pregnancy than women in the pre-pandemic group. GDM was diagnosed significantly earlier but in later pregnancies in the pandemic group than in the pre-pandemic group. The course of pregnancy in both groups was similar and the percentage of pregnancies with the delivery of neonates > 4000 g did not differ significantly. The number of caesarean sections and spontaneous deliveries was also similar in both periods. No differences were found in anthropometric parameters in newborns and neonatal and maternal complications. Conclusions: The occurrence of the COVID-19 pandemic and the necessity of employing telemedicine tools in the medical care of women with GDM did not significantly impact diabetes management and the pregnancy outcomes achieved.
Culture-Negative Native Vertebral Osteomyelitis: A Narrative Review of an Underdescribed Condition
Authors: Alavi Seyed Mohammad Amin , Petri Francesco , Mahmoud Omar K. , Igwilo-Alaneme Rita , El Zein Said , Nassr Ahmad N. , Gori Andrea , Berbari Elie F.
Published: September 2024

The incidence of culture-negative NVO (CN-NVO) cases is increasing, presenting significant diagnostic and therapeutic challenges due to the inability to isolate causative organisms with conventional microbiological methods. Factors influencing the diagnosis of CN-NVO include prior antimicrobial therapy, low pathogen burden, fastidious or intracellular organisms, technical issues, and non-infectious mimickers. Diagnosis often relies on imaging modalities like magnetic resonance imaging (MRI) and computed tomography (CT)-guided biopsy, though these methods can sometimes fail to yield positive microbiological results. Advanced diagnostic tools, such as polymerase chain reaction (PCR), metagenomic next-generation sequencing (mNGS), and cell-free DNA analysis, may be necessary to identify the pathogen. The causative pathogen cannot be isolated in some patients, among which an empirical antimicrobial therapy should be initiated. This narrative review discusses the management, monitoring, surgical indications, and outcomes for patients with CN-NVO.
Treatment of Psychological Symptoms in Patients with Cystic Fibrosis
Authors: Campagna Giovanna , Tagliati Corrado , Giuseppetti Gian Marco , Ripani Pietro
Published: September 2024

The aim of this article is to identify and illustrate the most used psychological techniques in the field of cystic fibrosis (CF) and to help clinicians choose the most appropriate strategy among various possibilities. The disease and its medical treatments can be difficult to tolerate and can cause anxiety about health status or feelings of hopelessness and stress. The prevalence of depression and anxiety is 2.3 times higher in adults with CF than in community samples. A strong correlation has been identified between elevated psychological distress and unfavorable health outcomes, including, among others, impaired lung function, reduced BMI, an increased incidence of pulmonary exacerbations, and an elevated risk of transplantation. The use of psychological interventions is useful in addressing these common distresses in CF patients. Aware of the necessity of identifying efficacious interventions for all levels of depression and anxiety in CF patients, this study presents an overview of the research on psychological interventions for patients with CF, in order to complement the treatments suggested by the international guidelines on mental health in CF cases. In fact, the aim of this study is to conduct a review and quantitative synthesis of the psychological intervention techniques that are currently available for individuals with CF.