bluuebunny/crossref_metadata_2025 huggingface dataset:
Network-based approach for targeting human kinases commonly associated with amyotrophic lateral sclerosis and cancer
Authors: Khatoon Fatima , Haque Shafiul , Hashem Anwar , Mahmoud Ahmad , Tashkandi Hanaa , Mathkor Darin , Harakeh Steve , Alghamdi Badra , Kumar Vijay
Published: December 2022

Background

Amyotrophic Lateral Sclerosis (ALS) is a rare progressive and chronic motor neuron degenerative disease for which at present no cure is available. In recent years, multiple genes encode kinases and other causative agents for ALS have been identified. Kinases are enzymes that show pleiotropic nature and regulate different signal transduction processes and pathways. The dysregulation of kinase activity results in dramatic changes in processes and causes many other human diseases including cancers.

Methods

In this study, we have adopted a network-based system biology approach to investigate the kinase-based molecular interplay between ALS and other human disorders. A list of 62 ALS-associated-kinases was first identified and then we identified the disease associated with them by scanning multiple disease-gene interaction databases to understand the link between the ALS-associated kinases and other disorders.

Results

An interaction network with 36 kinases and 381 different disorders associated with them was prepared, which represents the complexity and the comorbidity associated with the kinases. Further, we have identified 5 miRNAs targeting the majority of the kinases in the disease-causing network. The gene ontology and pathways enrichment analysis of those miRNAs were performed to understand their biological and molecular functions along with to identify the important pathways. We also identified 3 drug molecules that can perturb the disease-causing network by drug repurposing.

Conclusion

This network-based study presented hereby contributes to a better knowledge of the molecular underpinning of comorbidities associated with the kinases associated with the ALS disease and provides the potential therapeutic targets to disrupt the highly complex disease-causing network.
Undernutrition and associated factors among pregnant women in East Borena Zone, Liban District, Oromia regional state, Ethiopia
Authors: Arero Godana
Published: December 2022

Background

Undernutrition is cellular imbalance between supply of nutrients, energy and body’s demand to ensure growth, maintenance, and specific function. However, there was no study conducted earlier on this topic in East Borena Zone.

Objective

To assess the prevalence of undernutrition and associated factors among pregnant women in East Borena Zone, Liban District.

Method

A community-based cross-sectional study was conducted on 420 study participants from November 20 to December 2021. The systematic sampling technique and simple random sampling methods were used to select study participants. Data were double entered into Epi-info software version 7 and SPSS version 21 software for analysis. Descriptive statistics were used to describe the characteristics of study participants. Bivariate and multivariable logistic regressions were carried out to identify the association between independent and dependent variables by measuring the adjusted odds ratio and 95% confidence interval. P-values less than 0.05 were considered statistically significant.

Results

Prevalence of undernutrition among pregnant women was about (44.9%) of family monthly income [AOR = 8.72 (4.80, 15.83)], women’s decision-making autonomy [AOR = 0.40 (0.19, 0.82)], skipping meal [AOR = 2.62 (1.41, 4.89)], substance use [AOR = 2.01 (1.07, 3.77)], household food insecurity [AOR = 2.01 (1.06, 3.80)], lack of prenatal dietary advice [AOR = 2.73 (1.53, 4.89)], absence of household latrine [AOR = 9.23 (3.48, 24.46)], not participating health development army’s meeting at village level [AOR = 3.01 (1.57, 5.72)] and hand washing habit [AOR = 6.55 (3.02, 14.20)] had shown statistically significant association with undernutrition.

Conclusion

The prevalence of undernutrition among pregnant women was high income. Women’s decision-making autonomy, skipping meals, substances use, household food insecurity, lack of prenatal dietary advice, poor hand washing habit, lack household of latrine, and not participation in health development army’s meeting were found to be predictors of the undernutrition.
States of quinolinic acid excess in urine: A systematic review of human studies
Authors: Saade Marie Christelle , Clark Amanda J. , Parikh Samir M.
Published: December 2022

Introduction

Quinolinic acid is an intermediate compound derived from the metabolism of dietary tryptophan. Its accumulation has been reported in patients suffering a broad spectrum of diseases and conditions. In this manuscript, we present the results of a systematic review of research studies assessing urinary quinolinic acid in health and disease.

Methods

We performed a literature review using PubMed, Cochrane, and Scopus databases of all studies reporting data on urinary quinolinic acid in human subjects from December 1949 to January 2022.

Results

Fifty-seven articles met the inclusion criteria. In most of the reported studies, compared to the control group, quinolinic acid was shown to be at increased concentration in urine of patients suffering from different diseases and conditions. This metabolite was also demonstrated to correlate with the severity of certain diseases including juvenile idiopathic inflammatory myopathies, graft vs. host disease, autism spectrum disorder, and prostate cancer. In critically ill patients, elevated quinolinic acid in urine predicted a spectrum of adverse outcomes including hospital mortality.

Conclusion

Quinolinic acid has been implicated in the pathophysiology of multiple conditions. Its urinary accumulation appears to be a feature of acute physiological stress and several chronic diseases. The exact significance of these findings is still under investigation, and further studies are needed to reveal the subsequent implications of this accumulation.
Isolation, purification, and structural elucidation of Stropharia rugosoannulata polysaccharides with hypolipidemic effect
Authors: Gao Yinlu , Abuduaini Gulijiannaiti , Yang Chenhe , Zhang Shanshan , Zhang Yanrong , Fan Hongxiu , Teng Xu , Bao Chenligen , Liu Hongcheng , Wang Dawei , Liu Tingting
Published: December 2022

Stropharia rugosoannulata is a widely grown edible mushroom with a high nutritional value. S. rugosoannulata polysaccharides is one of the most important bioactive components of S. rugosoannulata and has a wide range of activities. A S. rugosoannulata polysaccharides, named SRF-3, was derived from the S. rugosoannulata extraction by freeze-thaw combine with hot water extraction method, then prepareed with DEAE-cellulose column and Sephacryl S-200 HR gel column, and its hypolipidemic activity was determined. The structural characteristics of SRF-3 were analyzed by infrared spectral scanning (FT-IR), ultra-high performance liquid chromatography (UHPLC), acid hydrolysis, methylation analysis, nuclear magnetic resonance (NMR), and Gas Chromatography-Mass Spectrometer (GC-MS). SRF-3 is composed of mannose, galactose, methyl galactose and fructose with ratios of 16, 12, 58 and 12, respectively. In addition, the average relative molecular mass of SRF-3 is approximately 24 kDa. The main chain of SRF-3 is mainly composed of repeating α-D-1,6-Galp and α-D-1,6-Me-Galp units, with branches in the O-2 position of Gal. The structure is presumed to be a mannogalactan, with a small amount of t-β-D-Manp present as a side chain. Hypolipidemic activity assay showed that SRF-3 had good antioxidant and hypolipidemic effects in vitro, suggesting that SRF-3 have potential application in reducing liver fat accumulation.
Prediction of bile duct injury after transarterial chemoembolization for hepatocellular carcinoma: Model establishment and verification
Authors: Guo Jianxi , Zhang Xueying , Kong Jian
Published: December 2022

Objective

This study aimed to establish and validate a predictive model for bile duct injury in patients with hepatocellular carcinoma (HCC) after drug-eluting bead transarterial chemoembolization (DEB-TACE).

Methods

We retrospectively analyzed 284 patients with HCC treated with DEB-TACE at our hospital between January 2017 and December 2021, of whom 63 patients experienced postoperative bile duct injuries. Univariate and logistic multivariate regression analyses were performed to identify the risk factors for bile duct injury, as well as establish and internally validate the nomogram model. The area under the curve (AUC) of the receiver operating characteristic (ROC) curve, calibration curve, Hosmer-Lemeshow goodness of fit test, decision curve analysis (DCA), and clinical impact curve (CIC) were used to assess the predictive power, clinical value, and practicability of the nomogram model.

Results

The incidence of bile duct injuries after DEB-TACE was 22.18% (63/284), with one injury occurring in every 2.86 sessions of DEB-TACE treatment. Univariate and logistic multivariate regression analyses indicated that a history of hepatectomy (odds ratio [OR]=2.285; 95% confidence interval [CI]=1.066–4.898; P<0.05), subjective angiographic chemoembolization endpoint level (OR=1.832; 95% CI=1.258–2.667; P<0.05), alkaline phosphatase (OR=1.005; 95% CI=1.001–1.010; P<0.05), and platelet count (OR=1.005; 95% CI=1.001–1.009; P<0.05) were independent risk factors for bile duct injury after DEB-TACE among patients with HCC. The risk nomogram model based on the above four variables was validated using the bootstrap method, showing consistency between the predicted and experimental values. Furthermore, the model performed well in the Hosmer-Lemeshow goodness-of-fit test (2=3.648; P=0.887). The AUC of this model was 0.749 (95% CI=0.682–0.817), with an overall accuracy of 69.01%, a positive predictive value of 73.02%, a negative predictive value of 67.87%, a sensitivity of 73.0%, and a specificity of 67.90%, suggesting that the nomogram model had good accuracy and discrimination. In addition, DCA and CIC revealed a high clinical value and practicability of the model.

Conclusion

Bile duct injury in patients with HCC treated with DEB-TACE is caused by multiple factors rather than a single factor. The nomogram prediction model used in this study had a good fitting degree and prediction efficacy, with high clinical value and practicability.
A study on skin tumor classification based on dense convolutional networks with fused metadata
Authors: Yin Wenjun , Huang Jianhua , Chen Jianlin , Ji Yuanfa
Published: December 2022

Skin cancer is the most common cause of death in humans. Statistics show that competent dermatologists have a diagnostic accuracy rate of less than 80%, while inexperienced dermatologists have a diagnostic accuracy rate of less than 60%. The higher rate of misdiagnosis will cause many patients to miss the most effective treatment window, risking the patients’ life safety. However, the majority of the current study of neural network-based skin cancer diagnosis remains at the image level without patient clinical data. A deep convolutional network incorporating clinical patient metadata of skin cancer is presented to realize the classification model of skin cancer in order to further increase the accuracy of skin cancer diagnosis. There are three basic steps in the approach. First, the high-level features (edge features, color features, texture features, form features, etc.). Implied by the image were retrieved using the pre-trained DenseNet-169 model on the ImageNet dataset. Second, the MetaNet module is introduced, which uses metadata to control a certain portion of each feature channel in the DenseNet-169 network in order to produce weighted features. The MetaBlock module was added at the same time to improve the features retrieved from photos using metadata, choosing the most pertinent characteristics in accordance with the metadata data. The features of the MetaNet and MetaBlock modules were finally combined to create the MD-Net module, which was then used as input into the classifier to get the classification results for skin cancers. On the PAD-UFES-20 and ISIC 2019 datasets, the suggested methodology was assessed. The DenseNet-169 network model combined with this module, according to experimental data, obtains 81.4% in the balancing accuracy index, and its diagnostic accuracy is up between 8% and 15.6% compared to earlier efforts. Additionally, it solves the problem of actinic keratosis and poorly classified skin fibromas.
Direct Comparison of Schlieren and Soot Foil Measurements of Detonation Cell Sizes
Authors: Carter Mick , Blunck David L.
Published: December 2022

Detonation-based combustion cycles have the potential to have higher thermodynamic efficiencies than the more common deflagration-based combustion cycles because the pressure of the products is higher than that of the reactants. The geometry to which a detonation is confined can have a strong influence on the detonation’s behavior, or even prevent a detonation from occurring. A key measurement that can be used to design detonation-based engines is the cell size of the mixture. The cell size is a characteristic length scale of a chemical mixture. For the first time, this study compares two methods of collecting cell size measurements within a single tube. This approach controls for the effects of tube geometry and surface roughness, which may confound studies whose schlieren and soot foil measurements have been collected from different tubes. This study indicates that measurements taken using the two measurement techniques agree to within experimental uncertainty (a difference of 1.3 mm, or 7%). Soot foil methods are generally preferable for detonation cell size measurements because soot foils provide larger triple point sample sizes per detonation and lower instrumentation costs relative to schlieren methods. Various sources of uncertainty are extensively analyzed and reported for the two techniques.
Nonsurgical molding of congenital auricular deformities and analysis of the correction outcomes: A single-center, retrospective study in east China
Authors: Liu Chuanbo , Wo Peibin , Zhang Jufang , Li Jinsheng
Published: December 2022

Objective

Our research was carried out to provide a clinical reference for the application of nonsurgical therapy in newborns with congenital auricular deformities in east China.

Methods

A retrospective study of consecutive newborns using noninvasive ear molding was conducted in Hangzhou in east China's Zhejiang Province. The demographic and clinical information and photographs of the ear before and after treatment were taken. The diagnosis of each auricular deformity was identified, and the treatment outcome was evaluated.

Results

A total of 224 patients including 356 congenital ear anomalies received noninvasive ear molding. The median age of infants to initiate treatment was 39.5 days. The median treatment duration was 42.5 days. The median follow-up time was 137.0 days. The overall treatment effective rate of all infants with nonoperative ear molding was 92.1%, and mild skin irritation and ulceration occurred in 34 ear deformities (9.6%). It confirmed that the treatment efficiency was satisfactory and the complication rate was still acceptable despite the late initiation treatment of neonates in east China. Further analysis of treatment outcomes among three subgroups of infants (the ages to initiate the ear molding were respectively less than or equal to 28, 29–56, and more than 57 days) revealed that initiation treatment was significantly related to the treatment results and the earlier the initiation treatment, the higher the effective rate and the lower the complication incidence.

Conclusion

Our study hints that newborns in east China may have a longer period for correction. What is more, although our study affirmed a longer period for noninvasive molding, early diagnosis and treatment are still recommended to improve therapy efficiency and reduce treatment duration and complications.
Case report: Cerebellar swelling and hydrocephalus in familial hemophagocytic lymphohistiocytosis
Authors: Yoshida Taro , Moriya Kunihiko , Oikawa Keisuke , Miura Shoko , Asakura Yoshiko , Tanifuji Sachiko , Kusano Shuji , Endo Mikiya , Akasaka Manami
Published: December 2022

Familial hemophagocytic lymphohistiocytosis (FHL) is a severe inborn error of immunity caused by a genetic defect that impairs the function of cytotoxic T and NK cells. There are only a few reported cases of FHL with diffuse swelling of the cerebellum and obstructive hydrocephalus. We report a case of FHL3 with neurological symptoms associated with cerebellar swelling and obstructive hydrocephalus. A male patient was hospitalized several times due to fever and decreased feeding, hepatosplenomegaly, and cytopenia since the first month of life. At 7 months of age, disturbance of consciousness was seen. Brain magnetic resonance imaging revealed signal intensity in the bilateral cerebellar hemispheres, diffusely increased periventricular white matter, and ventriculomegaly. Although he was treated with methylprednisolone pulse therapy, he was unresponsive to the treatment. He was then transferred to a local hospital after tracheotomy but died. Targeted clinical sequencing revealed a homozygous splice-site mutation in UNC13D. Pediatric hemophagocytic lymphohistiocytosis (HLH) includes some cases of central nervous symptom (CNS)-isolated HLH or CNS HLH preceding systemic lesions, which often do not initially meet the diagnostic criteria for FHL. Patients with FHL initiated by cerebellar symptoms may present with an atypical clinical course for HLH, leading to delayed diagnosis and poor outcomes. Despite the usefulness of a combination of a high percentage of lymphocytes in the peripheral leukocytes, a low lactate dehydrogenase level, and a high sIL-2R/ferritin ratio for identifying FHL, the diagnosis may be missed due to the absence of these results. Presymptomatic diagnosis of FHL by screening of newborns and subsequent early treatment of patients with a predicted poor prognosis may contribute to better outcomes.
Quercetin and polycystic ovary syndrome
Authors: Ma Congshun , Xiang Qianru , Song Ge , Wang Xuefei
Published: December 2022

Polycystic ovary syndrome (PCOS) is a reproductive endocrine disease, and results to opsomenorrhea or amenorrhea, hairy, acne, acanthosis, infertility, abortion. In the long term, PCOS may also increase the risk of endometrial cancer, diabetes, hypertension, dyslipidemia and other diseases. Till now there is no specific drug for PCOS due to the unclearness of the cause and pathogenesis, as current treatments for PCOS only target certain symptoms. Quercetin (QUR) is a flavonoid drug widely found in Chinese herbal medicines, fruits, leaves, vegetables, seeds and plants roots. Studies on other diseases have found that QUR has anti-oxidant, anti-inflammatory, anti-insulin resistance, anti-cancer and other effects. Some studies have shown that serum testosterone (T), luteinizing hormone (LH), the LH/follicule-stimulating hormone (FSH) ratio, fasting glucose, fasting insulin, HOMA-IR and lipid levels are reduced in PCOS patients with QUR treatment. However, the mechanisms of QUR in PCOS patients have not been completely elucidated. In this review, we retrospect the basic characteristics of QUR, and in vitro studies, animal experiments and clinical trials of QUR and plant extracts containing QUR in the treatment of PCOS. We also summarized the effects and mechanism of QUR in ovarian cells in vitro and PCOS model rats, the changes in relevant parameters after QUR administration in PCOS patients, and its potentially therapeutic applications.
Small extracellular vesicles in metabolic remodeling of tumor cells: Cargos and translational application
Authors: Yang Hao , Wang Jingyi , Huang Gang
Published: December 2022

Warburg effect is characterized by excessive consumption of glucose by the tumor cells under both aerobic and hypoxic conditions. This metabolic reprogramming allows the tumor cells to adapt to the unique microenvironment and proliferate rapidly, and also promotes tumor metastasis and therapy resistance. Metabolic reprogramming of tumor cells is driven by the aberrant expression and activity of metabolic enzymes, which results in the accumulation of oncometabolites, and the hyperactivation of intracellular growth signals. Recent studies suggest that tumor-associated metabolic remodeling also depends on intercellular communication within the tumor microenvironment (TME). Small extracellular vesicles (sEVs), also known as exosomes, are smaller than 200 nm in diameter and are formed by the fusion of multivesicular bodies with the plasma membrane. The sEVs are instrumental in transporting cargoes such as proteins, nucleic acids or metabolites between the tumor, stromal and immune cells of the TME, and are thus involved in reprogramming the glucose metabolism of recipient cells. In this review, we have summarized the biogenesis and functions of sEVs and metabolic cargos, and the mechanisms through they drive the Warburg effect. Furthermore, the potential applications of targeting sEV-mediated metabolic pathways in tumor liquid biopsy, imaging diagnosis and drug development have also been discussed.
Calycosin induces autophagy and apoptosis via Sestrin2/AMPK/mTOR in human papillary thyroid cancer cells
Authors: Qu Na , Qu Junsheng , Huang Na , Zhang Kexin , Ye Tongtong , Shi Junfeng , Chen Bing , Kan Chengxia , Zhang Jingwen , Han Fang , Hou Ningning , Sun Xiaodong , Pan Ruiyan
Published: December 2022

Calycosin, one of small molecules derived from astragalus, has anti-tumor effects in various tumors. However, the effects of calycosin on papillary thyroid cancer (PTC) remain unclear. This study aimed to explore the anti-tumor ability of calycosin on human PTC and its potential mechanisms. The B-CPAP cells were treated with calycosin, then cell proliferation, apoptosis and invasiveness were measured by CCK8 assay, flow cytometry, wound healing and transwell invasion assay, respectively. The cells were also performed by whole transcriptome microarray bioinformatics analysis. Apoptosis and autophagy-related markers or proteins were measured by qRT-PCR or western blot. Sestrin2-mediated AMPK/mTOR pathways were determined by western blot. We found that calycosin inhibited migration and invasion of B-CPAP cells and induced apoptosis (Bax/Bcl-2) and autophagy (LC3II/I, Beclin1) of B-CPAP cells. Differential expressed genes were screened between the calycosin-treated cells and control (524 genes upregulated and 328 genes downregulated). The pathway enrichment suggested that the role of calycosin in B-CPAP cells is closely related to apoptosis-related genes and p70S6 Kinase. Transmission electron microscopy found an increase in autophagosomes in calycosin-treated cells. Sestrin2 in human PTC tissues and B-CPAP cells was lower than in normal thyroid tissues and cells. And the pharmacological effects of calycosin in PTC cells were related to Sestrin2 activation, increased p-AMPK and inhibited p-mTOR and p-p70S6Kinase; these alterations were reversed when silencing Sestrin2. In conclusion, calycosin has an inhibitory effect on PTC via promoting apoptosis and autophagy through the Sestrin2/AMPK/mTOR pathway.
The efficacy and safety of Yupingfeng Powder with variation in the treatment of allergic rhinitis: Study protocol for a randomized, double-blind, placebo-controlled trial
Authors: Cheong Pui Kuan , Ho Tin Muk , Chan Kam Leung , Lo Cho Wing , Leung Sin Bond , Hon Kam Lun , Leung Ka Chun , Siu Tony Hon Chung , Song Tian-He , Zhang Hongwei , Ching Jessica Yuet Ling , Chow Tak Yee , Sum Chi Him , Chia Chon Pin , Lin Zhi-Xiu
Published: December 2022

Background: Allergic rhinitis (AR) is an upper airways chronic inflammatory disease mediated by IgE, which affects 10%–20% of the population. The mainstay for allergic rhinitis nowadays include steroids and antihistamines, but their effects are less than ideal. Many patients therefore seek Chinese medicine for treatment and Yupingfeng Powder is one of the most common formulae prescribed. In this study, we aim to investigate the efficacy and safety of Yupingfeng Powder with variation for the treatment of allergic rhinitis.

Study design: This is a double-blind, randomized, placebo-controlled trial. A 2-week screening period will be implemented, and then eligible subjects with allergic rhinitis will receive interventions of either “Yupingfeng Powder with variation” granules or placebo granules for 8 weeks, followed by post treatment visits at weeks 12 and 16. The change in the Total Nasal Symptom Score (TNSS) will be used as the primary outcome.

Discussion: This trail will evaluate the efficacy and safety of Yupingfeng Powder in treating allergic rhinitis. The study may provide the solid evidence of Yupingfeng Powder with variation can produce better clinical efficacy than the placebo granules.

Trial registration:ClinicalTrials.gov, identifier NCT04976023.
Halophyte Nitraria billardieri CIPK25 promotes photosynthesis in Arabidopsis under salt stress
Authors: Lu Lu , Wu Xinru , Tang Yao , Zhu Liming , Hao Zhaodong , Zhang Jingbo , Li Xinle , Shi Jisen , Chen Jinhui , Cheng Tielong
Published: December 2022

The calcineurin B-like (CBL)-interacting protein kinases (CIPKs), a type of plant-specific genes in the calcium signaling pathway, function in response to adverse environments. However, few halophyte derived CIPKs have been studied for their role in plant physiological and developmental adaptation during abiotic stresses, which inhibits the potential application of these genes to improve environmental adaptability of glycophytes. In this study, we constructed Nitraria billardieri CIPK25 overexpressing Arabidopsis and analyzed the seedling development under salt treatment. Our results show that Arabidopsis with NbCIPK25 expression exhibits more vigorous growth than wild type plants under salt condition. To gain insight into the molecular mechanisms underlying salt tolerance, we profiled the transcriptome of WT and transgenic plants via RNA-seq. GO and KEGG analyses revealed that upregulated genes in NbCIPK25 overexpressing seedlings under salt stress are enriched in photosynthesis related terms; Calvin-cycle genes including glyceraldehyde-3-phosphate dehydrogenases (GAPDHs) are significantly upregulated in transgenic plants, which is consistent with a decreased level of NADPH (GAPDH substrate) and increased level of NADP+. Accordingly, NbCIPK25 overexpressing plants exhibited more efficient photosynthesis; soluble sugar and proteins, as photosynthesis products, showed a higher accumulation in transgenic plants. These results provide molecular insight into how NbCIPK25 promotes the expression of genes involved in photosynthesis, thereby maintaining plant growth under salt stress. Our finding supports the potential application of halophyte-derived NbCIPK25 in genetic modification for better salt adaptation.
The roles of species’ relatedness and climate of origin in determining optical leaf traits over a large set of taxa growing at high elevation and high latitude
Authors: Hartikainen Saara M. , Robson T. Matthew
Published: December 2022

Climate change is driving many mountain plant species to higher elevations and northern plant species to higher latitudes. However, various biotic or abiotic constraints may restrict any range shift, and one relevant factor for migration to higher elevations could be species’ ability to tolerate high UV-doses. Flavonoids are engaged in photoprotection, but also serve multiple ecological roles. We compared plant optical leaf trait responses of a large set of taxa growing in two botanical gardens (French Alps and southern Finland), considering potential constraints imposed by the relatedness of taxa and the legacy of climatic conditions at plants’ original collection sites. The segregation of optically measured leaf traits along the phylogeny was studied using a published mega-tree GBOTB.extended.tre for vascular plants as a backbone. For a subset of taxa, we investigated the relationship between climatic conditions (namely solar radiation, temperature and precipitation at a coarse scale) at the plants’ original collection site and current trait values. Upon testing the phylogenetic signal (Pagel’s λ), we found a significant difference but intermediate lambda values overall for flavonol or flavone index (Iflav) and anthocyanin index (Iant), indicating that phylogenetic relatedness alone failed to explain the changes in trait values under a Brownian motion model of trait evolution. The local analysis (local indicator of phylogenetic association) indicated mostly positive autocorrelations for Iflavi.e. similarities in optically measured leaf traits, often among species from the same genus. We found significant relationships between climatic variables and leaf chlorophyll index (Ichl), but not Iflav, particularly for annual solar radiation. Changes in plants’ Iflavacross microhabitats differing in UV irradiance and predominately highFv/Fmindicated that most plants studied had sufficient flexibility in photoprotection, conferred by Iflav, to acclimate to contemporary UV irradiances in their environment. While not explaining the mechanisms behind observed trait values, our findings do suggest that some high-elevation taxa display similar leaf flavonoid accumulation responses. These may be phylogenetically constrained and hence moderate plants’ capacity to adjust to new combinations of environmental conditions resulting from climate change.
Gene expression and DNA methylation altering lead to the high oil content in wild allotetraploid peanut (A. monticola)
Authors: Liu Nian , Wu Bei , Pandey Manish K. , Huang Li , Luo Huaiyong , Chen Yuning , Zhou Xiaojing , Chen Weigang , Huai Dongxin , Yu Bolun , Chen Hao , Guo Jianbin , Lei Yong , Liao Boshou , Varshney Rajeev K. , Jiang Huifang
Published: December 2022

Introduction

The wild allotetraploid peanut Arachis monticola contains a higher oil content than the cultivated allotetraploid Arachis hypogaea. Besides the fact that increasing oil content is the most important peanut breeding objective, a proper understanding of its molecular mechanism controlling oil accumulation is still lacking.

Methods

We investigated this aspect by performing comparative transcriptomics from developing seeds between three wild and five cultivated peanut varieties.

Results

The analyses not only showed species-specific grouping transcriptional profiles but also detected two gene clusters with divergent expression patterns between two species enriched in lipid metabolism. Further analysis revealed that expression alteration of lipid metabolic genes with co-expressed transcription factors in wild peanut led to enhanced activity of oil biogenesis and retarded the rate of lipid degradation. In addition, bisulfite sequencing was conducted to characterize the variation of DNA methylation between wild allotetraploid (245, WH 10025) and cultivated allotetraploid (Z16, Zhh 7720) genotypes. CG and CHG context methylation was found to antagonistically correlate with gene expression during seed development. Differentially methylated region analysis and transgenic assay further illustrated that variations of DNA methylation between wild and cultivated peanuts could affect the oil content via altering the expression of peroxisomal acyl transporter protein (Araip.H6S1B).

Discussion

From the results, we deduced that DNA methylation may negatively regulate lipid metabolic genes and transcription factors to subtly affect oil accumulation divergence between wild and cultivated peanuts. Our work provided the first glimpse on the regulatory mechanism of gene expression altering for oil accumulation in wild peanut and gene resources for future breeding applications.
Combating powdery mildew: Advances in molecular interactions between Blumeria graminis f. sp. tritici and wheat
Authors: Mapuranga Johannes , Chang Jiaying , Yang Wenxiang
Published: December 2022

Wheat powdery mildew caused by a biotrophic fungus Blumeria graminis f. sp. tritici (Bgt), is a widespread airborne disease which continues to threaten global wheat production. One of the most chemical-free and cost-effective approaches for the management of wheat powdery mildew is the exploitation of resistant cultivars. Accumulating evidence has reported that more than 100 powdery mildew resistance genes or alleles mapping to 63 different loci (Pm1-Pm68) have been identified from common wheat and its wild relatives, and only a few of them have been cloned so far. However, continuous emergence of new pathogen races with novel degrees of virulence renders wheat resistance genes ineffective. An essential breeding strategy for achieving more durable resistance is the pyramiding of resistance genes into a single genotype. The genetics of host-pathogen interactions integrated with temperature conditions and the interaction between resistance genes and their corresponding pathogen a virulence genes or other resistance genes within the wheat genome determine the expression of resistance genes. Considerable progress has been made in revealing Bgt pathogenesis mechanisms, identification of resistance genes and breeding of wheat powdery mildew resistant cultivars. A detailed understanding of the molecular interactions between wheat and Bgt will facilitate the development of novel and effective approaches for controlling powdery mildew. This review gives a succinct overview of the molecular basis of interactions between wheat and Bgt, and wheat defense mechanisms against Bgt infection. It will also unleash the unsung roles of epigenetic processes, autophagy and silicon in wheat resistance to Bgt.
Optimization of callus induction and proliferation of Paeonia lactiflora Pall. and Agrobacterium-mediated genetic transformation
Authors: Duan Siyang , Xin Rujie , Guan Shixin , Li Xueting , Fei Riwen , Cheng Wan , Pan Qing , Sun Xiaomei
Published: December 2022

Paeonia lactiflora Pall. is an important ornamental plant with high economic and medicinal value, which has considerable development prospects worldwide. The lack of efficient tissue culture techniques and genetic transformation systems has become a master obstacle for P. lactiflora research. The purpose of the present study focuses on obtaining an efficient and stable genetic transformation method using callus as the receptor and exploring an efficient protocol for callus induction and proliferation associated with P. lactiflora. Callus induction and proliferation were performed using MS medium with various concentrations of 2,4-Dichlorophenoxyacetic acid (2,4-D), 1-Naphthaleneacetic acid (NAA), 6-Benzylaminopurine (6-BA) and thidiazuron (TDZ). The sensitivity of callus to kanamycin and cefotaxime was determined. Several parameters such as Agrobacterium cell density, infection time and co-culture duration were studied to optimize transformation efficiency. Agrobacterium strains EHA105 and pBI121 binary vector harboring the β-glucuronidase (GUS) gene were used for transformation. Expression of the GUS reporter gene was detected by GUS assay, polymerase chain reaction (PCR) and Quantitative Real-time PCR (RT-qPCR). The MS medium containing 1.0 mg·L-1 NAA, 0.5 mg·L-1 2,4-D and 0.5 mg·L-1 TDZ was optimal for callus induction and MS medium containing 0.5 mg·L-1 NAA, 1.0 mg·L-1 2,4-D and 0.5 mg·L-1 TDZ was the best for callus proliferation. The concentrations of kanamycin and cefotaxime used for screening positive callus were 125 mg·L-1 and 200 mg·L-1, respectively. Among various combinations analyzed, the best transformation result was obtained via the 25 min of infection of Agrobacterium at 0.6 OD600 and 3 d of co-culture. Overall, this study provided technical support and theoretical guidance for improving the callus induction and proliferation efficiency and the study of gene function in P. lactiflora.
Influencing factors of farmers’ participation in domestic waste classification: An empirical analysis based on the semi-nonparametric estimation extended model
Authors: Sun Qianlu , Duan Huiqing , Zhong Daojun
Published: December 2022

Farmers are the main participants of domestic waste classification, and their willingness and behavior to participate are directly related to the success or failure of domestic waste classification and the construction of “beautiful countryside.” Based on the analysis of the influence mechanism of exemplary behavior and social supervision on farmers’ participation willingness and behavior, an empirical analysis of 988 survey data of farmers in Henan Province is carried out using a semi-non-parametric estimation extended model. The results show that: (1) 85.63% of farmers are willing to participate in the classification of domestic waste, but their willingness and behavior are not consistent. (2) The exemplary behavior of relatives can only increase the willingness of farmers. The exemplary behavior of neighbors and village cadres not only has a positive impact on the behavior, but also facilitates the transformation of willingness to behavior. (3) The supervision of village cadres can increase the willingness of farmers. Although the supervision of villagers and cleaners will reduce the willingness of farmers, it has a significant positive impact on the behavior of farmers. Based on the research conclusions, suggestions are made to play the leading role of village cadres, attach importance to the supervision of villagers and cleaners, broaden publicity channels and strengthen publicity to special groups, improve supporting policies and classification equipment, in order to promote the classification of rural domestic waste.
Perceived maternal disapproval of friends: How mothers shape and respond to child and friend adjustment problems
Authors: Kaniušonytė Goda , Žukauskienė Rita , Bakaitytė Aistė , Laursen Brett
Published: December 2022

The present study examines relations between adjustment problems and perceptions of maternal disapproval of friends in a sample of Lithuanian public middle-school students. The participants (ages 10 to 14) were 284 children (148 boys, 136 girls) who were involved in 142 stable friendships. Each friend described their own conduct problems, emotional problems, and perceptions of maternal disapproval of friends twice during the same academic year (M = 14.4 weeks apart). Dyadic analyses replicated previous findings in that one friend’s conduct and emotional problems forecast changes in the same problems in the other friend. Greater initial problems also anticipated increases in children’s’ perceptions of disapproval of friends by their own—but not their friend’s—mother, highlighting maternal efforts to manage the relations of troubled children. These efforts met with mix success. On the one hand, maternal disapproval of friends did not result in subsequent declines in adjustment problems among their own children; to the contrary, the conduct problems of boys increased with greater maternal involvement. On the other hand, adjustment problems declined among children whose friends reported higher levels of maternal disapproval, suggesting that maternal friendship management may interfere with the spread of problems between children.
System quality, information quality, satisfaction and acceptance of online learning platform among college students in the context of online learning and blended learning
Authors: Li Xiaoxia , Zhu Wanxia
Published: December 2022

The paper was based on the User Satisfaction and Technology Acceptance Integration Theory (USATA). The authors analyzed the factors that affect college students’ acceptance and satisfaction of online learning platform, as well as the differences in the relationship between various factors in blended learning scenario and online learning scenario. The results showed that the quality of online learning platform and information quality affect user satisfaction, and satisfaction affects usefulness and ease of use, and then affect attitude and intention. The comparison between the two groups showed that there were significant differences in the impact of information quality on information satisfaction and the impact of perceived usefulness on usage intention. In the online learning scenario, the endogenous latent variables of the model had higher explanatory power, which indicates that learners are more dependent on the quality and relevant characteristics of the learning platform in the online learning scenario.
Continuous norming in learning progress monitoring—An example for a test in spelling from grade 2–4
Authors: Schurig Michael , Blumenthal Stefan , Gebhardt Markus
Published: December 2022

One of the main goals of the teacher and the school system as a whole is to close learning gaps and support children with difficulties in learning. The identification of those children as well as the monitoring of their progress in learning is crucial for this task. The derivation of comparative standards that can be applied well in practice is a relevant quality criterion in this context. Continuous normalization is particularly useful for progress monitoring tests that can be conducted at different points in time. Areas that were not available in the normalization sample are extrapolated, closing gaps in applicability due to discontinuity. In Germany, teachers participated in a state-funded research project to formatively measure their children's spelling performance in primary school. Data (N= 3000) from grade two to four were scaled, linked and translated into comparative values that can be used in classrooms independently from specific times. The tests meet the requirements of item response models and can be transferred well to continuous norms. However, we recommend using the 10th or 20th percentile as cut-off points for educational measures, as the 5th percentile is not discriminating enough.
Demographic and work-related factors associated with burnout, resilience, and quality of life among healthcare workers during the COVID-19 pandemic: A cross sectional study from Malaysia
Authors: Marzo Roy Rillera , ElSherif Mohamed , Abdullah Muhd Siv Azhar Merican Bin , Thew Hui Zhu , Chong Collins , Soh Shean Yih , Siau Ching Sin , Chauhan Shekhar , Lin Yulan
Published: December 2022

Introduction

The healthcare setting is a stressful and demanding work environment, and healthcare workers face a continuous expansion of their job roles and responsibilities. Past studies have shown that factors affecting burnout, resilience, and quality of life among healthcare workers merit further research, as there were inconsistent findings, especially with regards to the influence of demographic and work-related factors. Therefore, this study aims to determine whether demographic and work-related factors are associated with burnout, resilience, and quality of life among healthcare workers.

Method

This cross-sectional study was conducted between February 15, 2022 and March 15, 2022, among 394 healthcare workers from Putrajaya and Selangor hospitals, Malaysia. Maslach Burnout Inventory, World Health Organization Quality of Life-BREF 26 inventory, and Brief Resilience Scale were utilized to capture information on burnout, quality of life, and resilience, respectively.

Results

The mean score of physical health of participants who work more than 10 h (11.38) is lower than participants who work from 8 to 10 h (13.00) and participants who work 7 h daily (13.03), p-value < 0.001. Similarly, the mean score of psychological health of participants who work more than 10 h (12.35) is lower than participants who work from 8 to 10 h (13.72) and participants who work 7 h daily (13.68), p-value = 0.001. Higher income levels were associated with high resilience and quality of life.

Conclusion

It is imperative that healthcare practitioners and policy makers adopt and implement interventions to promote a healthy workplace environment, address ethical concerns, and prevent burnout among healthcare workers during the COVID-19 pandemic. Managing the issue of long working hours could possibly result in improved resilience, burnout, and quality of life among healthcare workers. Despite this study able to tickle out some policy specific areas where interventions are needed, identifying effective solutions and evaluating their efficiency will require larger and interventional studies.
Will personality traits affect the use of e-cigar among college students? A cross-sectional study in Guangdong Province, China
Authors: Mai Jianrong , Lin Lina , Zhou Ling , Guan Qinyi , Zhu Wenhui , Zhou Wenzan
Published: December 2022

Background

The prevalence of e-cigar among adolescents and young adults around the world is increasing rapidly, which has a serious impact on the health of young people. This study assessed the prevalence of e-cigar among college students and to explore the relationship between e-cigar use and personality traits.

Methods

This study conducted an electronic questionnaire survey on college students who were from three undergraduate universities and three junior colleges in Guangdong Province from January 2022 to March 2022. The survey was conducted by stratified cluster sampling, and the respondents were 1362. Statistical descriptions are used to describe the demographic characteristics and personality traits of participants. Mann-Whitney U tests, and Chi-square tests were used to compare the differences between current e-cigar users and non e-cigar users. Two-step hierarchical Logistic regression was used to predict the associated factors with e-cigar use.

Results

The prevalence of current e-cigar users was 5.1%. Agreeableness showed statistically significant higher in non-users (Z = 2.585, P < 0.01). Moreover, gender (AOR = 0.312, 95%CI: 0.174–0.562), the relationship with mother (AOR = 5.887, 95%CI: 1.460–23.748), friends who use e-cigar (AOR = 3.808, 95%CI: 2.159–6.719), allowance per month (AOR = 2.482, 95%CI: 1.371–4.490), and agreeableness (AOR = 0.957, 95%CI: 0.918–0.997) were related to the use of e-cigar.

Conclusion

The level of agreeableness is associated with the use of e-cigar among college students. All these provided an important theoretical basis for future intervention.
Incidence and determinants of hysterectomy among North Indian women: An 8-year follow-up study
Authors: Rajkumari Sunanda , Chaudhary Vineet , Kasaudhan Sapana , Saraswathy Kallur Nava
Published: December 2022

Background

Despite indications of a rapid increase in the number of hysterectomies performed in India, very few studies have methodically investigated the rate and determinants of the incidence of hysterectomy. The present study aims to estimate the rate of incidence of hysterectomy and identify predictors/determinants of incident hysterectomy in a cohort of North Indian women.

Methods

In the present study, a cohort of 1,009 ever-married North Indian women (aged 30–75 years) was followed up after a median of 8.11 years. Those hysterectomized at the baseline (63) were excluded; and of the rest 946 participants, 702 (74.2%) could be successfully followed-up. During the baseline assessment, data about sociodemographic variables, reproductive history, menopausal status, physiological health, and selected blood biochemicals were collected. During the end-line assessment, data about sociodemographic variables, current menopausal status, and incident hysterectomy were recorded.

Results

The overall rate of incidence of hysterectomy was found to be 11.59 per 1,000 women-years, in the study population. Interestingly, the incidence rates were found to be similar among pre- and post-menopausal women. Further, while late age at menarche was found to be negatively associated with incident hysterectomy, folate repletion and high triglyceride (TG) at the baseline were found to be positively associated.

Conclusions

High rate of incident hysterectomy in the studied population points toward the huge burden of gynecological morbidity and the unavailability of non-invasive protocols. Such a situation warrants immediate policy intervention. Further, maintaining TG and folate within normal physiological ranges may be beneficial in gynecological ailments necessitating hysterectomy.