bluuebunny/crossref_metadata_2025 huggingface dataset:
Sports and Social Interaction: Sports Experiences and Attitudes of the Urban Running Community
Authors: Yang Jia , Ju Fang-Yuan , Tian Zu-Guo
Published: November 2022

Social change and development influence the motivational factors of people’s sports participation, exposing the need for socialization and interaction. The purpose of this study is to investigate the interaction pattern of the urban running community and the behavior pattern of runners with the help of social interaction theory, and to explore the inner connection between their community movement and social interaction. Ten senior members of marathon urban running societies were selected to conduct two rounds of in-depth interviews which were analyzed using qualitative thematic analysis to understand the sports participation experiences and social interactions of running society members. The study found that the whole interaction process of running groups is accomplished through three aspects: examination of self, adjustment with reference to others, and a sense of group belonging as the core consciousness. On the other hand, the social attributes of running groups can advance the personalization process of runners in society, which is mainly characterized by self-presentation and external constraints, self-requirement and group constraints, intergroup interaction and group identity reorganization. In addition, the unique community culture, standardized group organization and harmonious activity atmosphere will stimulate runners’ interest in running, strengthen community communication and establish stable community relations, etc., which in turn will bring about continuous interactive behaviors.
Structural Interventions to Enable Adolescent Contraceptive Use in LMICs: A Mid-Range Theory to Support Intervention Development and Evaluation
Authors: Burchett Helen Elizabeth Denise , Griffin Sally , de Melo Málica , Picardo Joelma Joaquim , Kneale Dylan , French Rebecca S.
Published: November 2022

Enabling contraceptive use is critical for addressing high adolescent pregnancy rates in low- and middle-income countries (LMICs). Broader or ‘upstream’ determinants, such as poverty, education, and social norms, can affect the knowledge, attitudes, motivation, and ability to access and use contraception. Structural interventions aim to address these broader determinants, e.g., through poverty alleviation from livelihood training or cash transfers, increasing school participation, or changing social norms. We conducted an evidence synthesis using intervention component analysis, a case-based approach, following a systematic mapping of the evidence base. We identified 17 studies with 29 structural intervention arms, which reported adolescent contraceptive use outcomes compared to a control group or baseline. It was not possible to identify with certainty which interventions were ‘likely effective’ or ‘likely ineffective’ due to the high heterogeneity of the methods. We built on an existing framework of family planning use to propose three steps to designing interventions: (1) tailor interventions to adolescents’ life stages; (2) assess the baseline situation; and (3) select appropriate activities to match the gaps. These steps will aid developers and evaluators of structural adolescent contraceptive interventions to develop an evidence base that is of use across a wide range of settings and use scenarios.
Healthy Eats—Evaluation of a Social Marketing Program Delivered in Primary School Settings in Queensland
Authors: Isbanner Sebastian , Carins Julia , Rundle-Thiele Sharyn
Published: November 2022

One in four school children in Australia are overweight or obese. In response, the Healthy Eats program was developed, piloted, and delivered using a whole-of-school approach underpinned by the socio-ecological model to increase fruit and vegetable consumption among children aged 8–10 years in regional Queensland, Australia. This research presents an outcome evaluation of the Healthy Eats program using pre–post data collected throughout 2021 (cross-sectional for knowledge and longitudinal for behaviour) from 19 schools to assess whether changes occurred in students’ nutritional knowledge (n = 1868 (pre = 933, post = 935)) and fruit and vegetable consumption (n = 1042 (pre = 521, post = 521)). Knowledge data was collected via self-reports two weeks prior and immediately after the Nutrition Module. Behavioural data on daily fruit and vegetable consumption was gathered via student passports (i.e., surveys) one week before and for four consecutive weeks after the Nutrition Module. Chi-Square Difference tests and t-Tests were conducted with a significance level set at p < 0.05. Across all 19 schools, knowledge of the daily recommended serves of fruit and vegetables improved significantly following participation in the program, aligning knowledge closer to the Australian dietary guidelines. Behavioural results for fruit consumption were favourable, with clear improvements reported. Increases in vegetable consumption were demonstrated in two of the eight schools. A discussion on the knowledge–action gap is provided, including recommendations for future iterations of the Healthy Eats program.
A Geographical and Temporal Risk Evaluation Method for Red-Light Violations by Pedestrians at Signalized Intersections: Analysis and Results of Suzhou, China
Authors: Xing Jiping , Zhang Qi , Cheng Qixiu , Zu Zhenshan
Published: November 2022

Red-light violations of pedestrians crossing at signal intersections is one of the key factors in pedestrian traffic accidents. Even though there are various studies on pedestrian behavior and pedestrian traffic conflicts, few focus on the risk of different crosswalks for the violating pedestrian group. Due to the spatio-temporal nature of violation risk, this study proposes a geographical and temporal risk evaluation method for pedestrian red-light violations, which combines actual survey and video acquisition. First, in the geographical-based risk evaluation, the pedestrian violation rate at signal intersections is investigated by Pearson correlation analysis to extract the significant influencing factors from traffic conditions, built environment, and crosswalk facilities. Second, in the temporal-based risk evaluation, the survival analysis method is developed to quantify the risk of pedestrian violation in different scenarios as time passes by. Finally, this study selects 16 typical signalized intersections in Suzhou, China, with 881 pedestrian crosswalk violations from a total size of 4586 pedestrians as survey cases. Results indicate that crossing distance, traffic volume on the crosswalk, red-light time, and crosswalk-type variables all contribute to the effect of pedestrian violation from a geographical perspective, and the installation of waiting refuge islands has the most significant impact. From the temporal perspective, the increases in red-light time, number of lanes, and traffic volume have a mitigating effect on the violations with pedestrian waiting time increases. This study aims to provide a development-oriented path by proposing an analytical framework that reconsiders geographical and temporal risk factors of violation. The findings could help transport planners understand the effect of pedestrian violation-related traffic risk and develop operational measures and crosswalk design schemes for controlling pedestrian violations occurring in local communities.
Nurse Leadership and Work Environment Association with Outcome Expectancy and Self-Efficacy in Evidence-Based Practice among Hospital Nurses in The Netherlands: A Cross-Sectional Study
Authors: Hoegen Peter , Vos Mireille , van Oostveen Catharina , de Bot Cindy , Echteld Michael A. , Maaskant Jolanda , Vermeulen Hester
Published: November 2022

The active participation of nurses in evidence-based practice (EBP) is challenging and topical, as shown by the worldwide calls for appropriate, accessible, affordable care and the de-implementation of unnecessary care. Nurses’ perceived support from their managers and work environments may affect their self-efficacy and outcome expectancy in EBP, as well as hinder them in EBP. Associations between these issues have not yet been explored. This study examines the association of self-efficacy and outcome expectancy levels in EBP, as well as the perceived support for EBP from nurse leaders and in the working environment, among Dutch hospital nurses. Methods. Questionnaires measuring nurses’ self-efficacy, outcome expectancy, and perceived support for EBP from nurse leaders and their work environment were completed by 306 nurses in eight hospitals between March 2021 and June 2021. We used multilevel regression analyses to determine the associations and covariates. Results. This study shows that EBP-supportive leaders and work environments positively contribute to nurses’ self-efficacy and outcome expectancy in EBP, along with the covariates undertaking EBP activities and educational level. Conclusions. To improve nurses’ active participation in EBP, nurses need to increase their self-efficacy and outcome expectancy in EBP. Supportive leaders and a supportive work environment do have an impact. Hence, these factors need attention when implementing EBP among nurses.
Developing Indicators of Age-Friendliness in Taiwanese Communities through a Modified Delphi Method
Authors: Huang Jo-Ying , Hsu Hui-Chuan , Hsiao Yu-Ling , Chen Feng-Yin , Lo Shu-Ying , Chou Tzu-Yun , Liu Megan F.
Published: November 2022

This study developed indicators of age-friendliness for communities in Taiwan that conform to international standards by referring to the World Health Organization Checklist of Essential Features of Age-Friendly Cities and Taiwan’s existing indicators. The first stage of the research was based on the WHO’s framework and involved a literature review to identify candidate indicators. In the second stage, experts’ opinions were collected through a modified Delphi method, and the indicators were screened and revised on the basis of their importance, community enforceability, and generality. The third stage focused on practical feasibility. External parties were invited to offer their opinions regarding the indicators, which were adjusted accordingly. After three rounds of review and re-examination based on the modified Delphi method, the final set of indicators comprised five core indicators and five optional indicators. These indicators can be used to monitor various aspects of communities and determine their age-friendliness.
Biopsychosocial Correlates of Quality of Life in Multiple Sclerosis Patients
Authors: Batista Ana Rita , Silva Susana , Lencastre Leonor , Guerra Marina Prista
Published: November 2022

Multiple sclerosis (MS) is a demyelinating chronic disease that has had increasing prevalence over the last years. We have investigated whether the perceived quality of life is reduced in multiple sclerosis patients compared to control participants with a cross-sectional approach, and how it relates to sociodemographic, clinical, and psychosocial variables in MS with multiple regression. To that end, a group of MS patients (n = 50) and a control group (n = 50) that was matched for age and education level filled in the WHOQOL-BREF (perceived quality of life across four domains) and a sociodemographic questionnaire. The participants in the MS group also filled in a clinical questionnaire and three instruments measuring psychosocial variables (the DASS-21 for depression, anxiety, and stress, the Brief-COPE for coping skills, and the Meaning in Life Scale). The results showed that the perceived quality of life was lower in the MS group than in the control group. Multiple regression models incorporating the variables that showed significant correlations with the quality of life indicated that age, professional status, recovery from relapses, depression, active coping, and meaning in life predicted at least one domain of the quality of life. Meaning in life predicted the quality of life in all four of the domains. Although the quality of life in MS is linked to multiple biopsychosocial variables, meaning in life seems crucial.
River Ecosystem Health Assessment Using a Combination Weighting Method: A Case Study of Beijing Section of Yongding River in China
Authors: Chen Linglong , Ma Lan , Jiji Jiamen , Kong Qingqi , Ni Zizhao , Yan Lin , Pan Chengzhong
Published: November 2022

(1) Background: River health assessment provides the foundation for sustainable river development and management. However, existing assessments have no uniform standards and methods. (2) Methods: The combination weighting method was proposed, drawing on the advantages of subjective and objective weighting methods. To comprehensively investigate the river health level, an index system based on 16 indices selected from river morphology, river water environment, riparian condition, and social services level was established. The method and framework were applied to the Beijing section of Yongding River in China. (3) Results: The comprehensive weights of river morphology, river water environment, riparian condition, and social services are 0.1614, 0.3170, 0.4459, and 0.0757, respectively. The river health comprehensive index of Yongding River is 3.805; the percentages of excellent, healthy, sub-healthy, unhealthy, and sick river segments are 0%, 11%, 69%, 20%, and 0%, respectively. (4) Conclusions: The results indicate that Yongding River is in a sub-healthy state, and the riparian condition is the key factor that affects the river ecosystem health. Health level exhibited a remarkable spatial variation, mainly influenced by anthropogenic activities, and effective measures are needed to minimize the impact in fragile ecological areas.
Chemical Structure of Stabilizing Layers of Negatively Charged Silver Nanoparticles as an Effector of Shifts in Soil Bacterial Microbiome under Short-Term Exposure
Authors: Przemieniecki Sebastian Wojciech , Oćwieja Magdalena , Ciesielski Sławomir , Halecki Wiktor , Matras Ewelina , Gorczyca Anna
Published: November 2022

In this work, we have assessed the exposure of soil bacteria from potato monoculture to three types of silver nanoparticles (AgNPs) as well as silver ions (Ag+ ions) delivered in the form of silver nitrate and a commercially available fungicide. The diversity of the soil microbial community, enzymatic activity, and carbon source utilization were evaluated. It was found that only the fungicide significantly limited the abundance and activity of soil bacteria. Silver ions significantly reduced bacterial metabolic activity. In turn, one type of AgNPs prepared with the use of tannic acid (TA) increased bacterial load and activity. There was found in all AgNPs treated soils (1) a greater proportion of all types of persistent bacteria, i.e., Bacillus, Paenibacillus, and Clostridium; (2) a visible decrease in the proportion of Nocardioides, Arthrobacter, and Candidatus Solibacter; (3) almost complete depletion of Pseudomonas; (4) increase in the number of low-frequency taxa and decrease in dominant taxa compared to the control soil. Despite the general trend of qualitative changes in the bacterial community, it was found that the differences in the chemical structure of the AgNP stabilizing layers had a significant impact on the specific metabolic activity resulting from qualitative changes in the microbiome.
Molecular Mechanisms Underlying Mimosa acutistipula Success in Amazonian Rehabilitating Minelands
Authors: Nascimento Sidney Vasconcelos do , Herrera Héctor , Costa Paulo Henrique de Oliveira , Trindade Felipe Costa , da Costa Isa Rebecca Chagas , Caldeira Cecílio Frois , Gastauer Markus , Ramos Silvio Junio , Oliveira Guilherme , Valadares Rafael Borges da Silva
Published: November 2022

Mimosa acutistipula is endemic to Brazil and grows in ferruginous outcrops (canga) in Serra dos Carajás, eastern Amazon, where one of the largest iron ore deposits in the world is located. Plants that develop in these ecosystems are subject to severe environmental conditions and must have adaptive mechanisms to grow and thrive in cangas. Mimosa acutistipula is a native species used to restore biodiversity in post-mining areas in canga. Understanding the molecular mechanisms involved in the adaptation of M. acutistipula in canga is essential to deduce the ability of native species to adapt to possible stressors in rehabilitating minelands over time. In this study, the root proteomic profiles of M. acutistipula grown in a native canga ecosystem and rehabilitating minelands were compared to identify essential proteins involved in the adaptation of this species in its native environment and that should enable its establishment in rehabilitating minelands. The results showed differentially abundant proteins, where 436 proteins with significant values (p < 0.05) and fold change ≥ 2 were more abundant in canga and 145 in roots from the rehabilitating minelands. Among them, a representative amount and diversity of proteins were related to responses to water deficit, heat, and responses to metal ions. Other identified proteins are involved in biocontrol activity against phytopathogens and symbiosis. This research provides insights into proteins involved in M. acutistipula responses to environmental stimuli, suggesting critical mechanisms to support the establishment of native canga plants in rehabilitating minelands over time.
Exercise, Mediterranean Diet Adherence or Both during Pregnancy to Prevent Postpartum Depression—GESTAFIT Trial Secondary Analyses
Authors: Flor-Alemany Marta , Migueles Jairo H. , Alemany-Arrebola Inmaculada , Aparicio Virginia A. , Baena-García Laura
Published: November 2022

Targeting lifestyle behaviors during pregnancy is crucial to prevent the highly prevalent postpartum depression and its consequences. In these secondary analyses of an intervention trial to investigate the effects of concurrent exercise training on postpartum depression, we aimed to investigate the potential role of Mediterranean diet (MD) adherence on the exercise effects. A total of 85 pregnant women met the per-protocol criteria (exercise n = 46, control n = 39). The exercise program was delivered in 60 min sessions, 3 days/week, from the 17th gestational week until birth. Women’s dietary habits were assessed with a food frequency questionnaire. The Mediterranean Food Pattern (an MD index) was derived from it to assess MD adherence. We used the Edinburgh Postnatal Depression Scale to assess postpartum depression. The postpartum depression score was not statistically different between control and exercise groups (p > 0.05). A higher consumption of fruits (β = −0.242, p = 0.022), lower intake of red meat and subproducts (β = 0.244, p = 0.020), and a greater MD adherence (β = −0.236, p = 0.027) were associated with lower levels of postpartum depression. Greater adherence to the MD during pregnancy was associated with fewer depressive symptoms and a lower risk of postpartum depression. Postnatal depression was not reduced by prenatal exercise. Promoting fruit consumption while controlling the intake of red meat during pregnancy might prevent postnatal depression.
Mesonephric-Like Adenocarcinomas a Rare Tumor: The Importance of Diagnosis
Authors: Restaino Stefano , Pellecchia Giulia , Tulisso Angelica , Paglietti Chiara , Orsaria Maria , Andreetta Claudia , Poletto Elena , Arcieri Martina , Martina Monica della , Biasioli Anna , Mariuzzi Laura , Driul Lorenza , Scambia Giovanni , Vizzielli Giuseppe
Published: November 2022

Mesonephric-like adenocarcinomas (MLA) are rare neoplasms that arise in the uterine body and ovary and have been added to the World Health Organisation’s recent 2020 classification of female genital cancers. The pathogenesis of MLA is unknown and it remains debated whether they represent mesonephric carcinomas (Wolffian) arising in the endometrium/ovary or endometrioid carcinomas (Müllerian) closely mimicking mesonephric carcinomas. Here we report the case of a 57-year-old woman with an initial misdiagnosis of endometrioid adenocarcinoma on diagnostic biopsy. The patient came to our clinical evaluation for the appearance of menometrorrhagia complicated by anemia for several months. Therefore, she underwent pelvic echo-flowmetry, with indication for diagnostic hysteroscopy with endometrial biopsy, which yielded a positive result for endometrioid endometrial adenocarcinoma. Following staging CT scan and targeted examinations on pulmonary findings, the patient underwent surgery with surprise of definitive diagnosis deponent for endometrial MLA. Our intention is to establish a brief review of the scientific evidence in the literature and the tools available for a correct histological diagnosis, in the light of the scant anatomopathological evidence. Our question gives rise to the motive for the publication: is immunohistochemistry the right way to resolve the diagnostic error at histology, which is usually the only source of diagnostic certainty? This case is intended to alert of diagnostic error that risked having the patient treated as a neoplasm with a favorable prognosis and low degree of aggressiveness instead of for a very aggressive and poor prognosis tumor such as MLA.
Human Mobility Restrictions and COVID-19 Infection Rates: Analysis of Mobility Data and Coronavirus Spread in Poland and Portugal
Authors: Strzelecki Artur , Azevedo Ana , Rizun Mariia , Rutecka Paulina , Zagała Kacper , Cicha Karina , Albuquerque Alexandra
Published: November 2022

This study examines the possibility of correlation between the data on human mobility restrictions and the COVID-19 infection rates in two European countries: Poland and Portugal. The aim of this study is to verify the correlation and causation between mobility changes and the infection spread as well as to investigate the impact of the introduced restrictions on changes in human mobility. The data were obtained from Google Community Mobility Reports, Apple Mobility Trends Reports, and The Humanitarian Data Exchange along with other reports published online. All the data were organized in one dataset, and three groups of variables were distinguished: restrictions, mobility, and intensity of the disease. The causal-comparative research design method is used for this study. The results show that in both countries the state restrictions reduced human mobility, with the strongest impact in places related to retail and recreation, grocery, pharmacy, and transit stations. At the same time, the data show that the increase in restrictions had strong positive correlation with stays in residential places both in Poland and Portugal.
Spatial Effect of Digital Economy on Particulate Matter 2.5 in the Process of Smart Cities: Evidence from Prefecture-Level Cities in China
Authors: Tan Jingrong , Chen Lin
Published: November 2022

During the COVID-19 pandemic, the digital economy has developed rapidly. The airborne nature of COVID-19 viruses has attracted worldwide attention. Therefore, it is of great significance to analyze the impact of the digital economy on particulate matter 2.5 (PM2.5) emissions. The research sample of this paper include 283 prefecture-level cities in China from 2011 to 2019 in China. Spatial Durbin model was adopted to explore the spatial spillover effect of digital economy on PM2.5 emissions. In addition, considering the impact of smart city pilot (SCP) policy, a spatial difference-in-differences (SDID) model was used to analyze policy effects. The estimation results indicated that (1) the development of the digital economy significantly reduces PM2.5 emissions. (2) The spatial spillover effect of the digital economy significantly reduces PM2.5 emissions in neighboring cities. (3) Smart city construction increases PM2.5 emissions in neighboring cities. (4) The reduction effect of the digital economy on PM2.5 is more pronounced in the sample of eastern cities and urban agglomerations.
Influence of Population Size, the Human Development Index and the Gross Domestic Product on Mortality by COVID-19 in the Southeast Region of Brazil
Authors: Groppo Mônica Feresini , Groppo Francisco Carlos , Figueroba Sidney Raimundo , Pereira Antonio Carlos
Published: November 2022

We evaluated the influence of population size (POP), HDI (Human Development Index) and GDP (gross domestic product) on the COVID-19 pandemic in the Southeast region of Brazil, between February 2020 and May 2021. Methods: Cases, deaths, incidence coefficient, mortality rate and lethality rate were compared among states. The cities were divided into strata according to POP, GDP, and HDI. Data were compared by Welch’s ANOVA, nonlinear polynomial regression, and Spearman’s correlation test (rS). Results: The highest incidence coefficient (p < 0.0001) and mortality rate (p < 0.05) were observed in the states of Espírito Santo and Rio de Janeiro, respectively. Until the 45th week, the higher the POP, the higher the mortality rate (p < 0.01), with no differences in the remaining period (p > 0.05). There was a strong positive correlation between POP size and the number of cases (rS = 0.92, p < 0.0001) and deaths (rS = 0.88, p < 0.0001). The incidence coefficient and mortality rate were lower (p < 0.0001) for low GDP cities. Both coefficients were higher in high- and very high HDI cities (p < 0.0001). The lethality rate was higher in the state of Rio de Janeiro (p < 0.0001), in large cities (p < 0.0001), in cities with medium GDP (p < 0.0001), and in those with high HDI (p < 0.05). Conclusions: Both incidence and mortality were affected by time, with minimal influence of POP, GDP and HDI.
Loneliness in Young Adult Workers
Authors: Wright Sarah L. , Silard Anthony G.
Published: November 2022

Loneliness is commonly associated with older people with the majority of research and interventions focusing on loneliness in aged and aging populations. However, loneliness seems to be on the rise for young adults more so than the elderly. Our research focusses on the experiences of young workers who report feeling lonely at work. We explore individual and organisational factors that may be contributing to loneliness, and comment on the consequences of feeling lonely at work. Qualitative data from 37 young adults from Western Europe suggest that these workers feel invisible at work, have a thwarted sense of belonging to their employing organisation, and often experience relational deficiencies due to automation and individualisation of work practices.
Menstrual and Reproductive Factors for Gastric Cancer in Postmenopausal Women: The 2007–2020 Korea National Health and Nutrition Examination Survey
Authors: Song Heekyoung , Park Jung Yoon , Song Ju Myung , Yoon Youngjae , Kim Yong-Wook
Published: November 2022

Globally, the incidence of gastric cancer is lower in women than in men. It is thought that menstrual and reproductive factors may be related to their lower incidence of gastric cancer. This cross-sectional study examined menstrual, reproductive, and other factors in 20,784 postmenopausal women from the 2007–2020 Korea National Health and Nutrition Examination Survey (KNHANES). A univariate logistic regression analysis was performed, and then a multivariate logistic regression analysis for significant factors in the univariate analysis was conducted. In the multivariate logistic regression analysis, the age at menarche (odds ratio [OR] 1.08, 95% confidence interval [CI] 1.00−1.06, p = 0.035) and myocardial infarction (OR 2.43, 95% CI 1.05−5.62, p = 0.026) showed a significant association with increased incidence of gastric cancer. The age at menopause (OR 0.97, 95% CI 0.95−1.00, p = 0.03), the age at the first childbirth (OR 0.93, CI 0.89−0.97, p = 0.007), and the experience of alcohol consumption (OR 0.68, 95% CI 0.5–0.91, p = 0.003) showed a significant association with a decreased incidence of gastric cancer. Late menarche, early menopause, early aged first childbirth, and myocardial infarction are estimated to be risk factors for gastric cancer in postmenopausal Korean women.
An Integrated Multi-Objective Optimization for Dynamic Airport Shuttle Bus Location, Route Design and Departure Frequency Setting Problem
Authors: Wei Ming , Yang Congxin , Liu Tao
Published: November 2022

An airport shuttle bus (ASB), as an environmentally friendly mode of green transportation, is an effective way to solve the “first/last mile” of aviation passengers, which can attract a higher passenger transfer from private cars to public transport, thereby reducing emissions of carbon dioxide and other polluting gases. This study presents a multi-objective mixed-integer linear programming for ASB services in a dynamic environment. Taking into account time-varying demand and travel time characteristics in different periods, the proposed model provides a comprehensive framework that simultaneously advises passengers to join the bus at the nearest bus stations, designs routes for transporting them from these selected stations through the airport, and computes their departure frequencies in multiple periods. The primary objective is to optimize both the total ride time and waiting time for all passengers. The secondary objective is to optimize the total transfer distance of all passengers simultaneously. Given the Non-Deterministic Polynomial (NP) hardness of this problem, a two-stage multi-objective heuristic approach based on the non-dominated sorting genetic algorithm (NSGA-II) is combined with a dynamic programming search method and further advanced to obtain the Pareto-optimal solutions of the proposed model within a reasonable time. Finally, the proposed model and algorithm feasibility are proved by a test example of designing a shuttle bus route and schedule at Tianjin Airport, China. The results show that the total passenger travel time of the presented model is markedly reduced by 1.21% compared with the conventional model.
Effects of Elevated Aluminum Concentration and Distribution on Root Damage, Cell Wall Polysaccharides, and Nutrient Uptake in Different Tolerant Eucalyptus Clones
Authors: Li Wannian , Ullah Saif , Xu Yuanyuan , Bai Tiandao , Ye Shaoming , Jiang Weixin , Yang Mei
Published: November 2022

Aluminized acidic soil can damage Eucalyptus roots and limit tree growth, hindering the productivity of Eucalyptus plantations. At present, the negative impacts of elevated aluminum (Al) on the cell morphology and cell wall properties of Eucalyptus root tip are still unclear. In order to investigate the responses of two different tolerant clones, Eucalyptus urophylla (G4) and Eucalyptus grandis × Eucalyptus urophylla (G9), to Al toxicity, seedling roots were treated hydroponically with an Al solution, and the polysaccharide content in the root tip cell wall and the characteristics of programmed cell death were studied. The results show that the distribution of Al was similar in both clones, although G9 was found to be more tolerant to Al toxicity than G4. The Al3+ uptake of pectin in root tip cell walls was significantly higher in G4 than in G9. The root tip in G4 was obviously damaged, enlarged, thickened, and shorter; the root crown cells were cracked and fluffy; and the cell elongation area was squeezed. The lower cell wall polysaccharide content and PME activity may result in fewer carboxylic groups in the root tip cell wall to serve as Al-binding sites, which may explain the stronger Al resistance of G9 than G4. The uptake of nitrogen and potassium in G4 was significantly reduced after aluminum application and was lower than in G9. Al-resistant Eucalyptus clones may have synergistic pleiotropic effects in resisting high aluminum–low phosphorus stress, and maintaining higher nitrogen and potassium levels in roots may be an important mechanism for effectively alleviating Al toxicity.
Preliminary Evidence of the Potent and Selective Adenosine A2B Receptor Antagonist PSB-603 in Reducing Obesity and Some of Its Associated Metabolic Disorders in Mice
Authors: Kotańska Magdalena , Dziubina Anna , Szafarz Małgorzata , Mika Kamil , Bednarski Marek , Nicosia Noemi , Temirak Ahmed , Müller Christa E. , Kieć-Kononowicz Katarzyna
Published: November 2022

The adenosine A2A and A2B receptors are promising therapeutic targets in the treatment of obesity and diabetes since the agonists and antagonists of these receptors have the potential to positively affect metabolic disorders. The present study investigated the link between body weight reduction, glucose homeostasis, and anti-inflammatory activity induced by a highly potent and specific adenosine A2B receptor antagonist, compound PSB-603. Mice were fed a high-fat diet for 14 weeks, and after 12 weeks, they were treated for 14 days intraperitoneally with the test compound. The A1/A2A/A2B receptor antagonist theophylline was used as a reference. Following two weeks of treatment, different biochemical parameters were determined, including total cholesterol, triglycerides, glucose, TNF-α, and IL-6 blood levels, as well as glucose and insulin tolerance. To avoid false positive results, mouse locomotor and spontaneous activities were assessed. Both theophylline and PSB-603 significantly reduced body weight in obese mice. Both compounds had no effects on glucose levels in the obese state; however, PSB-603, contrary to theophylline, significantly reduced triglycerides and total cholesterol blood levels. Thus, our observations showed that selective A2B adenosine receptor blockade has a more favourable effect on the lipid profile than nonselective inhibition.
Oligomerization of Human Cystatin C—An Amyloidogenic Protein: An Analysis of Small Oligomeric Subspecies
Authors: Wojciechowska Daria , Taube Michał , Rucińska Karolina , Maksim Joanna , Kozak Maciej
Published: November 2022

Human cystatin C (HCC), an amyloidogenic protein, forms dimers and higher oligomers (trimers, tetramers and donut like large oligomers) via a domain-swapping mechanism. The aim of this study was the characterization of the HCC oligomeric states observed within the pH range from 2.2 to 10.0 and also in conditions promoting oligomerization. The HCC oligomeric forms obtained in different conditions were characterized using size exclusion chromatography, dynamic light scattering and small-angle X-ray scattering. The marked ability of HCC to form tetramers at low pH (2.3 or 3.0) and dimers at pH 4.0–5.0 was observed. HCC remains monomeric at pH levels above 6.0. Based on the SAXS data, the structure of the HCC tetramer was proposed. Changes in the environment (from acid to neutral) induced a breakdown of the HCC tetramers to dimers. The tetrameric forms of human cystatin C are formed by the association of the dimers without a domain-swapping mechanism. These observations were confirmed by their dissociation to dimers at pH 7.4.
Spatial Memory Training Counteracts Hippocampal GIRK Channel Decrease in the Transgenic APPSw,Ind J9 Alzheimer’s Disease Mouse Model
Authors: Temprano-Carazo Sara , Contreras Ana , Saura Carlos A. , Navarro-López Juan D. , Jiménez-Díaz Lydia
Published: November 2022

G-protein-gated inwardly rectifying potassium (GIRK) channels are critical determinants of neuronal excitability. They have been proposed as potential targets to restore excitatory/inhibitory balance in acute amyloidosis models, where hyperexcitability is a hallmark. However, the role of GIRK signaling in transgenic mice models of Alzheimer’s disease (AD) is largely unknown. Here, we study whether progressive amyloid-β (Aβ) accumulation in the hippocampus during aging alters GIRK channel expression in mutant β-amyloid precursor protein (APPSw,Ind J9) transgenic AD mice. Additionally, we examine the impact of spatial memory training in a hippocampal-dependent task, on protein expression of GIRK subunits and Regulator of G-protein signaling 7 (RGS7) in the hippocampus of APPSw,Ind J9 mice. Firstly, we found a reduction in GIRK2 expression (the main neuronal GIRK channels subunit) in the hippocampus of 6-month-old APPSw,Ind J9 mice. Moreover, we found an aging effect on GIRK2 and GIRK3 subunits in both wild type (WT) and APPSw,Ind J9 mice. Finally, when 6-month-old animals were challenged to a spatial memory training, GIRK2 expression in the APPSw,Ind J9 mice were normalized to WT levels. Together, our results support the evidence that GIRK2 could account for the excitatory/inhibitory neurotransmission imbalance found in AD models, and training in a cognitive hippocampal dependent task may have therapeutic benefits of reversing this effect and lessen early AD deficits.
Characteristics of the Protocols Used in Electrical Pulse Stimulation of Cultured Cells for Mimicking In Vivo Exercise: A Systematic Review, Meta-Analysis, and Meta-Regression
Authors: Nintou Eleni , Karligiotou Eleni , Vliora Maria , Ioannou Leonidas G. , Flouris Andreas D.
Published: November 2022

While exercise benefits a wide spectrum of diseases and affects most tissues and organs, many aspects of its underlying mechanistic effects remain unsolved. In vitro exercise, mimicking neuronal signals leading to muscle contraction in vitro, can be a valuable tool to address this issue. Following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines for this systematic review and meta-analysis, we searched EMBASE and PubMed (from database inception to 4 February 2022) for relevant studies assessing in vitro exercise using electrical pulse stimulation to mimic exercise. Meta-analyses of mean differences and meta-regression analyses were conducted. Of 985 reports identified, 41 were eligible for analysis. We observed variability among existing protocols of in vitro exercise and heterogeneity among protocols of the same type of exercise. Our analyses showed that AMPK, Akt, IL-6, and PGC1a levels and glucose uptake increased in stimulated compared to non-stimulated cells, following the patterns of in vivo exercise, and that these effects correlated with the duration of stimulation. We conclude that in vitro exercise follows motifs of exercise in humans, allowing biological parameters, such as the aforementioned, to be valuable tools in defining the types of in vitro exercise. It might be useful in transferring obtained knowledge to human research.
A Global Picture of Molecular Changes Associated to LPS Treatment in THP-1 Derived Human Macrophages by Fourier Transform Infrared Microspectroscopy
Authors: Ami Diletta , Franco Ana Rita , Artusa Valentina , Mereghetti Paolo , Peri Francesco , Natalello Antonino
Published: November 2022

Macrophages are among the first immune cells involved in the initiation of the inflammatory response to protect the host from pathogens. THP-1 derived macrophages (TDM) are used as a model to study the pro-inflammatory effects of lipopolysaccharide (LPS) exposure. Intact TDM cells were analysed by Fourier transform infrared (FTIR) microspectroscopy, supported by multivariate analysis, to obtain a snapshot of the molecular events sparked by LPS stimulation in macrophage-like cells. This spectroscopic analysis enabled the untargeted identification of the most significant spectral components affected by the treatment, ascribable mainly to lipid, protein, and sulfated sugar bands, thus stressing the fundamental role of these classes of molecules in inflammation and in immune response. Our study, therefore, shows that FTIR microspectroscopy enabled the identification of spectroscopic markers of LPS stimulation and has the potential to become a tool to assess those global biochemical changes related to inflammatory and anti-inflammatory stimuli of synthetic and natural immunomodulators different from LPS.
EGFR T751_I759delinsN Mutation in Exon19 Detected by NGS but Not by Real-Time PCR in a Heavily-Treated Patient with NSCLC
Authors: Chang Zi-Ting , Chan Tien-Ming , Wu Chiao-En
Published: November 2022

The detection of driver gene mutations can determine appropriate treatment strategies for non-small cell lung cancer (NSCLC) by identifying the presence of an effective druggable target. Mutations in the gene encoding the epidermal growth factor receptor (EGFR) are common driver mutations in NSCLC that can be effectively targeted by the use of EGFR tyrosine kinase inhibitors (EGFR-TKIs). However, without the detection of driver mutations, appropriate therapeutic decisions cannot be made. The most commonly applied methods for detecting driver gene mutations are assays based on polymerase chain reaction (PCR). However, the underlying mechanism of PCR-based assays limits the detection of rare mutations. Therefore, patients harboring rare mutations may not receive optimal treatment. We report a heavily-treated patient with NSCLC who harbored a T751_I759delinsN mutation in exon 19 of EGFR that was not detected by real-time PCR but was successfully detected by next-generation sequencing (NGS). The detection of a driver mutation using NGS resulted in the administration of targeted therapy, leading to favorable progression-free survival for the patient. Our report highlights the importance and potential of routine NGS testing among NSCLC patients for whom traditional assays fail to detect driver mutations when determining treatment options.
The AP2 Transcription Factor BrSHINE3 Regulates Wax Accumulation in Nonheading Chinese Cabbage
Authors: Huo Zhaoyan , Xu Yang , Yuan Song , Chang Jiang , Li Shuhao , Wang Jinwei , Zhao Huanhuan , Xu Ru , Zhong Fenglin
Published: November 2022

Wax is an acellular structural substance attached to the surface of plant tissues. It forms a protective barrier on the epidermis of plants and plays an important role in resisting abiotic and biotic stresses. In this paper, nonheading Chinese cabbage varieties with and without wax powder were observed using scanning electron microscopy, and the surface of waxy plants was covered with a layer of densely arranged waxy crystals, thus differentiating them from the surface of waxless plants. A genetic analysis showed that wax powder formation in nonheading Chinese cabbage was controlled by a pair of dominant genes. A preliminary bulked segregant analysis sequencing (BSA-seq) assay showed that one gene was located at the end of chromosome A09. Within this interval, we identified BraA09000626, encoding an AP2 transcription factor homologous to Arabidopsis AtSHINE3, and we named it BrSHINE3. By comparing the CDS of the gene in the two parental plants, a 35 bp deletion in the BrSHINE3 gene of waxless plants resulted in a frameshift mutation. Tissue analysis showed that BrSHINE3 was expressed at significantly higher levels in waxy plant rosette stage petioles and bolting stage stems than in the tissues of waxless plants. We speculate that this deletion in BrSHINE3 bases in the waxless material may inhibit wax synthesis. The overexpression of BrSHINE3 in Arabidopsis induced the accumulation of wax on the stem surface, indicating that BrSHINE3 is a key gene that regulates the formation of wax powder in nonheading Chinese cabbage. The analysis of the subcellular localization showed that BrSHINE3 is mainly located in the nucleus and chloroplast of tobacco leaves, suggesting that the gene may function as a transcription factor. Subsequent transcriptome analysis of the homology of BrSHINE3 downstream genes in nonheading Chinese cabbage showed that these genes were downregulated in waxless materials. These findings provide a basis for a better understanding of the nonheading Chinese cabbage epidermal wax synthesis pathway and provide important information for the molecular-assisted breeding of nonheading Chinese cabbage.
Whole Genome Analyses Accurately Identify Neisseria spp. and Limit Taxonomic Ambiguity
Authors: Khoder May , Osman Marwan , Kassem Issmat I. , Rafei Rayane , Shahin Ahmad , Fournier Pierre Edouard , Rolain Jean-Marc , Hamze Monzer
Published: November 2022

Genome sequencing facilitates the study of bacterial taxonomy and allows the re-evaluation of the taxonomic relationships between species. Here, we aimed to analyze the draft genomes of four commensal Neisseria clinical isolates from the semen of infertile Lebanese men. To determine the phylogenetic relationships among these strains and other Neisseria spp. and to confirm their identity at the genomic level, we compared the genomes of these four isolates with the complete genome sequences of Neisseria gonorrhoeae and Neisseria meningitidis and the draft genomes of Neisseria flavescens, Neisseria perflava, Neisseria mucosa, and Neisseria macacae that are available in the NCBI Genbank database. Our findings revealed that the WGS analysis accurately identified and corroborated the matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) species identities of the Neisseria isolates. The combination of three well-established genome-based taxonomic tools (in silico DNA-DNA Hybridization, Ortho Average Nucleotide identity, and pangenomic studies) proved to be relatively the best identification approach. Notably, we also discovered that some Neisseria strains that are deposited in databases contain many taxonomical errors. The latter is very important and must be addressed to prevent misdiagnosis and missing emerging etiologies. We also highlight the need for robust cut-offs to delineate the species using genomic tools.
The IL-33/ST2 Pathway in Cerebral Malaria
Authors: Glineur Corine , Leleu Inès , Pied Sylviane
Published: November 2022

Interleukin-33 (IL-33) is an immunomodulatory cytokine which plays critical roles in tissue function and immune-mediated diseases. IL-33 is abundant within the brain and spinal cord tissues where it acts as a key cytokine to coordinate the exchange between the immune and central nervous system (CNS). In this review, we report the recent advances to our knowledge regarding the role of IL-33 and of its receptor ST2 in cerebral malaria, and in particular, we highlight the pivotal role that IL-33/ST2 signaling pathway could play in brain and cerebrospinal barriers permeability. IL-33 serum levels are significantly higher in children with severe Plasmodium falciparum malaria than children without complications or noninfected children. IL-33 levels are correlated with parasite load and strongly decrease with parasite clearance. We postulate that sequestration of infected erythrocytes or merozoites liberation from schizonts could amplify IL-33 production in endothelial cells, contributing either to malaria pathogenesis or recovery.
Improving Stability and Specificity of CRISPR/Cas9 System by Selective Modification of Guide RNAs with 2′-fluoro and Locked Nucleic Acid Nucleotides
Authors: Sakovina Lubov , Vokhtantsev Ivan , Vorobyeva Mariya , Vorobyev Pavel , Novopashina Darya
Published: November 2022

The genome editing approach using the components of the CRISPR/Cas system has found wide application in molecular biology, fundamental medicine and genetic engineering. A promising method is to increase the efficacy and specificity of CRISPR/Cas-based genome editing systems by modifying their components. Here, we designed and chemically synthesized guide RNAs (crRNA, tracrRNA and sgRNA) containing modified nucleotides (2’-O-methyl, 2’-fluoro, LNA—locked nucleic acid) or deoxyribonucleotides in certain positions. We compared their resistance to nuclease digestion and examined the DNA cleavage efficacy of the CRISPR/Cas9 system guided by these modified guide RNAs. The replacement of ribonucleotides with 2’-fluoro modified or LNA nucleotides increased the lifetime of the crRNAs, while other types of modification did not change their nuclease resistance. Modification of crRNA or tracrRNA preserved the efficacy of the CRISPR/Cas9 system. Otherwise, the CRISPR/Cas9 systems with modified sgRNA showed a remarkable loss of DNA cleavage efficacy. The kinetic constant of DNA cleavage was higher for the system with 2’-fluoro modified crRNA. The 2’-modification of crRNA also decreased the off-target effect upon in vitro dsDNA cleavage.