bluuebunny/crossref_metadata_2025 huggingface dataset:
Photon Fotocompositora: um estudo de caso sobre inovação tecnológica a partir do acervo de Orlando da Costa Ferreira
Authors: Mirabeau da Fonseca Neto Almir
Published: October 2022

Neste espaço nos debruçamos sobre exemplares do acervo do bibliólogo Orlando da Costa Ferreira buscando refletir sobre relações  da indústria gráfica com os processos projetuais, ou seja, trataremos de Design e tecnologia. A análise aqui apresentada é parte integrante da tese A paisagem gráfica de Orlando da Costa Ferreira, defendida em 2018 no Programa de Pós-Graduação em Design da Escola Superior de Desenho Industrial da Universidade do Estado do Rio de Janeiro (PPDEsdi/UERJ). Nessa tese pesquisamos, entre outras coisas, a trajetória de Orlando da Costa Ferreira através da sua obra, dos seus registros pessoais e do acervo reunido durante sua vida. Propusemos que o bibliólogo foi um precursor nos estudos da Memória gráfica brasileira, tanto por sua preocupação com a dimensão material do livro, quanto pela sua abordagem que considerava que processos produtivos e, respectivamente os agentes tecnológicos e humanos que participaram deles, são fatores primordiais para entender os processos históricos.
A hegemonia branca e o conhecimento excludente no Design: uma análise sobre referências profissionais e bibliográficas
Authors: França Daniela , Carvalho Ricardo Artur Pereira
Published: October 2022

Este artigo busca discutir práticas excludentes e opressoras presentes no ensino do Design ao evidenciar o predomínio e hegemonia do pensamento de brancos e europeus neste campo de conhecimentos. O artigo também pretende elencar alguns aspectos da história do campo que causaram e ainda contribuem para a manutenção de uma estrutura racista e excludente até os dias atuais. Para isto, foi realizado um levantamento e análise das ementas de disciplinas para identificar a procedência dos autores que compõem o currículo de um curso de design. Em seguida, foi aplicado um formulário de consulta sobre referências de design com a participação de estudantes, professores e profissionais. Ao fim, discute-se a composição do campo acadêmico do design, analisando-o criticamente quanto à sua falta de representatividade e necessidade de se introduzir os conceitos de decolonialidade, racismo estrutural e institucional.
A reapropriação da subjetividade e a pedagogia da autonomia em ações extensionistas no campo do design
Authors: Necyk Barbara , Coutinho André Da Silva
Published: October 2022

Este artigo tem por objetivo investigar se ações extensionistas como o evento Rolé Virtual na Cinelândia possuem a potencialidade de promover a reapropriação da subjetividade no contexto do ensino em design. Em paralelo, estabelecemos a relação entre expectativas e achados de um evento que acabou por incorporar premissas da pedagogia da autonomia, em Freire, em uma universidade pública. O evento remoto propiciou uma experiência imersiva na forma de um "passeio" virtual aos moldes da atividade de desenho de observação. Mediado por aplicativos digitais conectados à Internet, o evento configurou-se como uma apropriação tecnológica de um aplicativo comumente utilizado para geolocalização, o que ­ contribuiu para criação de formas de reapropriação da subjetividade.
Cuando falta el Equipo de Protección Personal para los trabajadores de la salud durante los brotes de COVID-19: conclusiones del Grupo de trabajo catalán COVID-19
Authors: Seco Orriols Julia , Rovira-Puig Marina , Roviró-Aliguer Berta , Salmen-Navarro Acran , Rosal-López Gustavo Adolfo , Orriols Rosa M.
Published: October 2022

Objetivos: Los trabajadores sanitarios de primera línea tienen un alto riesgo de infección por SARS-CoV-2. El objetivo de este estudio es garantizar la protección según el nivel riesgo cuando falta el equipo de protección personal (EPI).
Métodos: Al comienzo de la pandemia, se sugirió que el EPI era necesario para proteger a los trabajadores de la salud del COVID-19; pero dada la escasez, el EPI tuvo que ser reemplazado en algunos escenarios. Se establecieron tres niveles de protección dependiendo del nivel de riesgo de exposición al SARS-CoV-2. Se analizaron las mejores prácticas para su posterior implantación en todos los hospitales de Cataluña. A medida que avanzaba la primera ola, nos familiarizamos mejor con el comportamiento del virus y los procedimientos en formato de tablas se modificaron y adaptaron a los nuevos escenarios. Se produjo un punto de inflexión después de que se confirmara que la mayoría de las infecciones se debían a la transmisión por vía aérea. Este avance mostró la importancia de identificar nuevas medidas que pudieran garantizar la protección de las vías respiratorias.
Resultados: Se han establecido tres tablas generales con según el riesgo bajo, medio y alto de contagio de los trabajadores de la salud. Estos tres escenarios son un subgrupo de la categoría de riesgo muy alto, de acuerdo con la pirámide de caracterización del riesgo de SARS-CoV-2 de OSHA. Se ha identificado el EPI más adecuado para cada tarea o trabajo y se han dado alternativas ante la escasez de EPI.
Conclusiones: Se requieren EPI específicos para el sector sanitario. Muchos estudios realizados sobre EPI se basan en las características de los trabajos industriales y no consideran las especificidades del sector sanitario, que requiere un contacto cercano y prolongado con los pacientes.
Organising as a catalyst for improving work conditions among informal quarry workers in Ghana
Authors: Segbenya Moses , Akorsu Angela D. , Enu-Kwesi Francis , Saha Debdulal
Published: October 2022

Organising has been suggested as one way to achieve decent working conditions among informal workers. This study examines how informal workers in Ghana are using organising as a catalyst for improving their working conditions. The study adopted an exploratory qualitative research design, using interviews, focus group discussions, documentary review and observation. Qualitative interpretative analysis methods were used to analyse the data. The study found that welfare-based organisations served as a catalyst for trade union organising among quarry workers. Despite the challenges confronting them in organising, informal quarry workers were able to improve their working conditions through a collective agreement with management. Specific indicators of work improvement included appointment letters for job security, hours of work and overtime, wages and salary (compensation), and training and development of workers to enhance their skills and competencies. The study concluded that organising informal workers served as a catalyst for ensuring decent working conditions among quarry workers in Ghana. This leads to the recommendation that formal trade unions organising in the quarrying districts should be resourced to be able to organise informal quarry workers in the stone quarry sub-sector of Ghana to achieve decent work conditions for informal workers in Ghana.
Fiber laser system of 1550  nm femtosecond pulses with configurable properties for the two-photon excitation of transient currents in semiconductor detectors
Authors: Almagro-Ruiz Azahara , Pape Sebastian , Muñoz-Marco Héctor , Wiehe Moritz , Currás Esteban , Fernández-García Marcos , Moll Michael , Montero Raúl , Palomo Francisco Rogelio , Quintana Cristian , Vila Iván , Pérez-Millán Pere
Published: October 2022

A fiber laser system emitting ultrashort femtosecond pulses at 1550 nm with configurable properties has been developed as an excitation source for the two-photon absorption transient current technique (TPA-TCT). The modules of the system are designed to provide the optical specifications required at the output for localized characterization of semiconductor radiation detectors: variation of pulse energy between 10 nJ and

10

p
J

, variation of the pulse repetition rate from 8.2 MHz to single shot, and variation of pulse duration between 300 and 600 fs. The validity of the system as an excitation source in the TPA-TCT is demonstrated by measuring spatially resolved excited charge carriers in a silicon detector.
Modified rigorous coupled-wave analysis for multi-layer deformable gratings with arbitrary profiles and materials
Authors: Zhang Caorui , Hu Fangjing , Fan Ji
Published: October 2022

In this paper, the rigorous coupled-wave analysis (RCWA) is extended for general multi-layer deformable gratings with arbitrary numbers of layers, surface profiles, layer offsets, and materials. The contribution from the offset between grating layers and/or due to the movement of the deformable grating layer is included in the expansion of the relative permittivity by the Fourier series, enabling the calculations of deformable gratings commonly used in many optical-based displacement sensing devices. The accuracy and efficiency of the extended RCWA are verified by a number of grating models. It is found that the numerical results are in excellent agreement with those from the finite element method, while the RCWA method costs only

∼

1

/

10

in computation time when compared to its counterpart. Our approach can be used for fast calculation and optimization of multi-layer deformable gratings for optical displacement sensing applications.
Scalable-resolution structured illumination microscopy
Authors: Butola Ankit , Acuna Sebastian , Hansen Daniel Henry , Agarwal Krishna
Published: October 2022

Structured illumination microscopy suffers from the need of sophisticated instrumentation and precise calibration. This makes structured illumination microscopes costly and skill-dependent. We present a novel approach to realize super-resolution structured illumination microscopy using an alignment non-critical illumination system and a reconstruction algorithm that does not need illumination information. The optical system is designed to encode higher order frequency components of the specimen by projecting PSF-modulated binary patterns for illuminating the sample plane, which do not have clean Fourier peaks conventionally used in structured illumination microscopy. These patterns fold high frequency content of sample into the measurements in an obfuscated manner, which are de-obfuscated using multiple signal classification algorithm. This algorithm eliminates the need of clean peaks in illumination and the knowledge of illumination patterns, which makes instrumentation simple and flexible for use with a variety of microscope objective lenses. We present a variety of experimental results on beads and cell samples to demonstrate resolution enhancement by a factor of 2.6 to 3.4 times, which is better than the enhancement supported by the conventional linear structure illumination microscopy where the same objective lens is used for structured illumination as well as collection of light. We show that the same system can be used in SIM configuration with different collection objective lenses without any careful re-calibration or realignment, thereby supporting a range of resolutions with the same system.
Optimization of the conversion efficiency and evaluation of the noise figure of an optical frequency converter based on a dual-pump fiber phase sensitive amplifier
Authors: Chatterjee Debanuj , Bouasria Yousra , Goldfarb Fabienne , Hassouni Yassine , Bretenaker Fabien
Published: October 2022

We propose a new architecture of phase sensitive optical frequency converter based on dual-pump phase sensitive amplification in a highly nonlinear fiber. This frequency converter allows generation of extra tones through nonlinear four-wave mixing between two strong pumps and an input tone. The frequency channel to which the input tone is converted can be chosen by adjusting the phase of the input signal. The conversion efficiency and extinction ratio of this frequency converter are predicted and optimized and its noise figure is calculated using a numerical approach based on the nonlinear Schrödinger equation. A semi-classical noise figure calculation for this approach was used and validated using an analytical fully quantum calculation based on the multi-wave model.
Reconstruction algorithm of haze image based on blind separation model of polarized orthogonal airlight
Authors: Zhang Xudong , Song Mingyue , Fan Zhiguo , Jin Haihong
Published: October 2022

Polarization-based dehazing methods can enhance the quality of haze images. However, existing methods tend to a manual selection of sky area and bias coefficient to estimate the degree of polarization (DoP) of the airlight, which leads to inaccurate estimation of the airlight. Aiming at the problem, a reconstruction algorithm based on the blind separation model of polarized orthogonal airlight is proposed. Importantly, the depth-dependent DoP of the airlight is automatically estimated without manual selection of sky area and bias coefficient. To reduce the interference of white objects on the estimation of airlight at infinity, an adaptive estimation method using the deviation between the DoP of the airlight and incident light is proposed. In order to accurate estimate the airlight from the airlight at infinity, a blind separation model of the airlight with multi-regularization constraints is established based on the decomposition of the airlight at infinity into a pair of polarized components with orthogonal angles. The experimental results show that the method effectively improves the visibility of scenes under different haze concentrations, especially in dense or heavy haze weather.
Nano-strain resolution fiber-optic Fabry-Perot sensors compatible with moderate/low resolution VIS-NIR spectrometers
Authors: Pevec Simon , Donlagic Denis
Published: October 2022

This paper reports on nano-strain resolution fiber-optic Fabry-Perot sensors produced by an improved selective etching method. The presented sensors exhibit high spectral sensitivity, low intrinsic temperature response, small size and mounting comparable to conventional Fiber Bragg gratings. Furthermore, the proposed sensors can be read-out by a combination of cost-efficient and widely available VIS/NIR spectrometers and LEDs used in lighting/automotive applications. A strain resolution of 1 nɛ was demonstrated when using a high-end FBG signal interrogator, while the application of a cost-efficient VIS spectrometer still yielded a strain resolution of about 20-70 nɛ. When applying suitable temperature compensation, absolute measurements with the nano-strain range are also plausible.
Blue and white light modulation of a flexible electroluminescent device based on phosphors
Authors: He Kun , Yu Yongjie , Cai Muzhi , Xu Haibo , Chen Liang , Xu Shiqing , Bai Gongxun
Published: October 2022

Flexibility, certain mechanical strength, and color modulation are significant elements for flexible optoelectronic devices. However, it is laborious to fabricate a flexible electroluminescent device with balanceable flexibility and color modulation. Here, we mix a conductive nonopaque hydrogel and phosphors to fabricate a flexible alternating current electroluminescence (ACEL) device with color modulation ability. This device realizes flexible strain based on polydimethylsiloxane and carboxymethyl cellulose/polyvinyl alcohol ionic conductive hydrogel. The color modulation ability is achieved by varying the voltage frequency applied on the electroluminescent phosphors. The color modulation could realize blue and white light modulation. Our electroluminescent device exhibits great potential in artificial flexible optoelectronics.
Ab initio predictions for 3D structure and stability of single- and double-stranded DNAs in ion solutions
Authors: Mu Zi-Chun , Tan Ya-Lan , Zhang Ben-Gong , Liu Jie , Shi Ya-Zhou
Published: October 2022

The three-dimensional (3D) structure and stability of DNA are essential to understand/control their biological functions and aid the development of novel materials. In this work, we present a coarse-grained (CG) model for DNA based on the RNA CG model proposed by us, to predict 3D structures and stability for both dsDNA and ssDNA from the sequence. Combined with a Monte Carlo simulated annealing algorithm and CG force fields involving the sequence-dependent base-pairing/stacking interactions and an implicit electrostatic potential, the present model successfully folds 20 dsDNAs (≤52nt) and 20 ssDNAs (≤74nt) into the corresponding native-like structures just from their sequences, with an overall mean RMSD of 3.4Å from the experimental structures. For DNAs with various lengths and sequences, the present model can make reliable predictions on stability, e.g., for 27 dsDNAs with/without bulge/internal loops and 24 ssDNAs including pseudoknot, the mean deviation of predicted melting temperatures from the corresponding experimental data is only ~2.0°C. Furthermore, the model also quantificationally predicts the effects of monovalent or divalent ions on the structure stability of ssDNAs/dsDNAs.
DPCfam: Unsupervised protein family classification by Density Peak Clustering of large sequence datasets
Authors: Russo Elena Tea , Barone Federico , Bateman Alex , Cozzini Stefano , Punta Marco , Laio Alessandro
Published: October 2022

Proteins that are known only at a sequence level outnumber those with an experimental characterization by orders of magnitude. Classifying protein regions (domains) into homologous families can generate testable functional hypotheses for yet unannotated sequences. Existing domain family resources typically use at least some degree of manual curation: they grow slowly over time and leave a large fraction of the protein sequence space unclassified. We here describe automatic clustering by Density Peak Clustering of UniRef50 v. 2017_07, a protein sequence database including approximately 23M sequences. We performed a radical re-implementation of a pipeline we previously developed in order to allow handling millions of sequences and data volumes of the order of 3 TeraBytes. The modified pipeline, which we call DPCfam, finds ∼ 45,000 protein clusters in UniRef50. Our automatic classification is in close correspondence to the ones of the Pfam and ECOD resources: in particular, about 81% of medium-large Pfam families and 72% of ECOD families can be mapped to clusters generated by DPCfam. In addition, our protocol finds more than 14,000 clusters constituted of protein regions with no Pfam annotation, which are therefore candidates for representing novel protein families. These results are made available to the scientific community through a dedicated repository.
Disruption of multiple copies of the Prostaglandin F2alpha synthase gene affects oxidative stress response and infectivity in Trypanosoma cruzi
Authors: Santi Ana Maria Murta , Ribeiro Juliana Martins , Reis-Cunha João Luís , Burle-Caldas Gabriela de Assis , Santos Isabella Fernandes Martins , Silva Paula Alves , Resende Daniela de Melo , Bartholomeu Daniella Castanheira , Teixeira Santuza Maria Ribeiro , Murta Silvane Maria Fonseca
Published: October 2022

Chagas disease, caused by the protozoan Trypanosoma cruzi, is a serious chronic parasitic disease, currently treated with Nifurtimox (NFX) and Benznidazole (BZ). In addition to high toxicity, these drugs have low healing efficacy, especially in the chronic phase of the disease. The existence of drug-resistant T. cruzi strains and the occurrence of cross-resistance between BZ and NFX have also been described. In this context, it is urgent to study the metabolism of these drugs in T. cruzi, to better understand the mechanisms of resistance. Prostaglandin F2α synthase (PGFS) is an enzyme that has been correlated with parasite resistance to BZ, but the mechanism by which resistance occurs is still unclear. Our results show that the genome of the CL Brener clone of T. cruzi, contains five PGFS sequences and three potential pseudogenes. Using CRISPR/Cas9 we generated knockout cell lines in which all PGFS sequences were disrupted, as shown by PCR and western blotting analyses. The PGFS deletion did not alter the growth of the parasites or their susceptibility to BZ and NFX when compared to wild-type (WT) parasites. Interestingly, NTR-1 transcripts were shown to be upregulated in ΔPGFS mutants. Furthermore, the ΔPGFS parasites were 1.6 to 1.7-fold less tolerant to oxidative stress generated by menadione, presented lower levels of lipid bodies than the control parasites during the stationary phase, and were less infective than control parasites.
A comparative analysis of current phasing and imputation software
Authors: De Marino Adriano , Mahmoud Abdallah Amr , Bose Madhuchanda , Bircan Karatuğ Ozan , Terpolovsky Andrew , Bamunusinghe Varuna , Bohn Sandra , Khan Umar , Novković Biljana , Yazdi Puya G.
Published: October 2022

Whole-genome data has become significantly more accessible over the last two decades. This can largely be attributed to both reduced sequencing costs and imputation models which make it possible to obtain nearly whole-genome data from less expensive genotyping methods, such as microarray chips. Although there are many different approaches to imputation, the Hidden Markov Model (HMM) remains the most widely used. In this study, we compared the latest versions of the most popular HMM-based tools for phasing and imputation: Beagle5.4, Eagle2.4.1, Shapeit4, Impute5 and Minimac4. We benchmarked them on four input datasets with three levels of chip density. We assessed each imputation software on the basis of accuracy, speed and memory usage, and showed how the choice of imputation accuracy metric can result in different interpretations. The highest average concordance rate was achieved by Beagle5.4, followed by Impute5 and Minimac4, using a reference-based approach during phasing and the highest density chip. IQS and R2 metrics revealed that Impute5 and Minimac4 obtained better results for low frequency markers, while Beagle5.4 remained more accurate for common markers (MAF>5%). Computational load as measured by run time was lower for Beagle5.4 than Minimac4 and Impute5, while Minimac4 utilized the least memory of the imputation tools we compared. ShapeIT4, used the least memory of the phasing tools examined with genotype chip data, while Eagle2.4.1 used the least memory phasing WGS data. Finally, we determined the combination of phasing software, imputation software, and reference panel, best suited for different situations and analysis needs and created an automated pipeline that provides a way for users to create customized chips designed to optimize their imputation results.
Accuracy evaluation of hand-eye calibration techniques for vision-guided robots
Authors: Enebuse Ikenna , Ibrahim Babul K. S. M. Kader , Foo Mathias , Matharu Ranveer S. , Ahmed Hafiz
Published: October 2022

Hand-eye calibration is an important step in controlling a vision-guided robot in applications like part assembly, bin picking and inspection operations etc. Many methods for estimating hand-eye transformations have been proposed in literature with varying degrees of complexity and accuracy. However, the success of a vision-guided application is highly impacted by the accuracy the hand-eye calibration of the vision system with the robot. The level of this accuracy depends on several factors such as rotation and translation noise, rotation and translation motion range that must be considered during calibration. Previous studies and benchmarking of the proposed algorithms have largely been focused on the combined effect of rotation and translation noise. This study provides insight on the impact of rotation and translation noise acting in isolation on the hand-eye calibration accuracy. This deviates from the most common method of assessing hand-eye calibration accuracy based on pose noise (combined rotation and translation noise). We also evaluated the impact of the robot motion range used during the hand-eye calibration operation which is rarely considered. We provide quantitative evaluation of our study using six commonly used algorithms from an implementation perspective. We comparatively analyse the performance of these algorithms through simulation case studies and experimental validation using the Universal Robot’s UR5e physical robots. Our results show that these different algorithms perform differently when the noise conditions vary rather than following a general trend. For example, the simultaneous methods are more resistant to rotation noise, whereas the separate methods are better at dealing with translation noise. Additionally, while increasing the robot rotation motion span during calibration enhances the accuracy of the separate methods, it has a negative effect on the simultaneous methods. Conversely, increasing the translation motion range improves the accuracy of simultaneous methods but degrades the accuracy of the separate methods. These findings suggest that those conditions should be considered when benchmarking algorithms or performing a calibration process for enhanced accuracy.
Estimate of the magnitude of risky and protective behaviors associated with road traffic injuries in capitals participating in the Life in Traffic Project of Brazil
Authors: Bazílio Gabriela Silvério , Guimarães Rafael Alves , Nazif-Munoz José Ignacio , Ouimet Marie Claude , Mamri Asma , Morais Neto Otaliba Libânio
Published: October 2022

Background

Brazil occupies the fifth position in the ranking of the highest mortality rates due to RTI in the world. With the objective of promoting traffic safety and consequently reducing deaths, Brazil created the Life in Traffic Project (LTP). The main goal of LTP is reducing 50% of RTI deaths, by promoting interventions to tackle risk factors, such as driving under the influence of alcohol and excessive and/or inappropriate speed. Thus, the aim of this study was to estimate the magnitude of risky and protective factors for RTI in capitals participating in the LTP in Brazil. We estimated these factors according to sociodemographic (age group, sex, education, race and, type of road user).

Methods

A total of 5,922 car drivers and motorcyclists from 14 Brazilian capitals participating in the LTP were interviewed. Data collection was carried out in sobriety checkpoints at night and consisted of the administration of an interview and a breathalyzer test. Risky and protective behaviors associated with RTI were investigated. Covariates of the study were: age, sex, education, race and, type of road user. Poisson multiple regression analysis was used to assess the relationship between variables of interest.

Results

The prevalence of individuals with positive blood alcohol concentration (BAC) was 6.3% and who reported driving after drinking alcohol in the last 30 days was 9.1%. The others risky behaviors reported were: driving at excessive speed on roads of 50 km/h, using a cell phone for calls while driving, using a cell phone to send or read calls while driving, running a red light. Use of seatbelts and helmets showed prevalence above 96,0% Use of seatbelts showed prevalence of 98.6% among car drivers, and helmet use was described by 96.6% of motorcycle drivers. Most risky behaviors were more prevalent in younger age groups (except BAC measurement higher in older participants), in males (except for cell phone use), in participants with higher education level and without a driver’s license.

Conclusion

Excessive speed and driving under the influence of alcohol, defined as priorities within the LTP, need more consistent interventions, as they still have considerable prevalence in the cities investigated. The factors described such as cell phone usage and passing red traffic lights should also need to be prioritized as a focus on promoting traffic safety.
Cardiac-derived extracellular matrix: A decellularization protocol for heart regeneration
Authors: Belviso Immacolata , Sacco Anna Maria , Cozzolino Domenico , Nurzynska Daria , Di Meglio Franca , Castaldo Clotilde , Romano Veronica
Published: October 2022

Extracellular matrix (ECM) is a fundamental component of the heart, guiding vital cellular processes during organ homeostasis. Most cardiovascular diseases lead to a remarkable remodeling of the ECM, accompanied by the formation of a fibrotic tissue that heavily compromises the heart function. Effective therapies for managing fibrosis and promoting physiological ECM repair are not yet available. The production of a decellularized extracellular matrix (d-ECM) serving as a three-dimensional and bioactive scaffold able to modulate cellular behavior and activities is considered crucial to achieve a successful regeneration. The protocol represents a step-by-step method to obtain a decellularized cardiac matrix through the combination of sodium dodecyl sulphate (SDS) and Triton X-100. Briefly, cardiac samples obtained from left ventricles of explanted, pathological human hearts were dissected and washed to remove residual body fluids. Samples were then snap-frozen and sliced by a cryostat into 350 μm thick sections. The sections obtained were decellularized using a solution containing 1% Triton X-100 and 1% SDS in combination, for 24 hours, until observing the color change from brownish-red to translucent-white. As a result, the protocol shows efficiency in preserving ECM architecture and protein composition during the whole process, suggesting that it is worthwhile, highly reproducible and produces a well- preserved decellularized extracellular matrix from cardiac samples. Notwithstanding, some limitations need to be addressed, such as the risk for microbial contamination and the unpredictable trend of the protocol when applied to decellularize samples other than myocardium, vessels, or skin. These issues require antibiotics mixture supplement during the procedure followed by UV sterilization, and appropriate adjustments for a tissue-specific utilization, respectively. The protocol is intended to produce a cardiac d-ECM for cell settlement, representing the ideal scaffold for tissue engineering purposes.
Equine echocardiography: Can dobutamine infusion correct alterations due to sedation with alpha-2 agonists?
Authors: Vitale Valentina , Vezzosi Tommaso , Di Franco Chiara , Briganti Angela , Tognetti Rosalba , Conte Giuseppe , Bucchioni Elena , Sgorbini Micaela
Published: October 2022

For the echocardiographic examination horses should not be sedated unless absolutely necessary because this alters cardiac dimensions and indices of function. However, some horses do not tolerate the echocardiographic procedure and require sedation to conduct the examination safely and obtain good quality images. The objective of this study was to evaluate whether the concurrent infusion of dobutamine in horses sedated with romifidine counteracts the cardiovascular changes observed with sedation alone. Twelve healthy untrained Standardbred mares were used. Three echocardiographic examinations were performed on the same day for each subject: a) without any treatment under resting conditions (WT); b) under sedation with romifidine administered intravenously (RT); c) under sedation with romifidine and concurrent intravenous infusion with dobutamine (RDT). A three-hour washout period was observed between each examination and the order of the examinations was randomly decided by rolling a dice. The measurements on the images recorded were performed offline at the end of the study protocol and at this point the operator was blinded to the horse and treatment administered. Left ventricular internal diameter (LVID) in diastole, left ventricular free wall (LVFW) in systole, and fractional shortening (FS) were higher in the WT group compared with the other two groups. No differences in the other M-mode and B-mode values were observed. A continuous rate infusion of dobutamine did not counteract the alterations caused by sedation and led to similar echocardiographic measurements to those obtained after romifidine administration.
Specific bacterial microbiome enhances the sexual reproduction and auxospore production of the marine diatom, Odontella
Authors: Sison-Mangus Marilou P. , Kempnich Michael W. , Appiano Monica , Mehic Sanjin , Yazzie Terril
Published: October 2022

Auxospore production is a sexual reproductive strategy by diatoms to re-attain normal size after the size-reducing effect of clonal reproduction. Aside from the minimum size threshold used as a sex clock by diatoms, the environmental or chemical triggers that can induce sex in diatoms are still not well understood. Here we investigated the influence of six marine bacteria from five families on the production of sexual cells and auxospores of the ubiquitous marine polar centric diatom, Odontella sp. Microbiome association and co-occurrence with the diatom in culture and in nature were investigated using 16S rRNA amplicon sequencing. Indole acetic acid (IAA) secretion, a phytohormone that regulates plants’ growth and sexual development, was explored as a potential inducer of sexual reproduction in Odontella and compared between bacterial associates. We found that Odontella co-cultured with Flavobacteriaceae (Polaribacter and Cellulophaga) have significantly more sexual cells and auxospores than bacteria-free Odontella and Odontella co-cultured with other bacteria from Vibrionaceae (Vibrio), Pseudoalteromonadaceae (Pseudoalteromonas), Rhodobacteraceae (Sulfitobacter), or Planococcaceae (Planococcus) family. Differences in IAA secretion were observed between bacterial isolates, but this did not correspond consistently with the diatom’s clonal growth or production of sexual cells and auxospores. Microbiome composition survey of Odontella cultures showed that the diatom harbors homologous sequences of the four bacterial isolates at varying proportions, with Sulfitobacter and Polaribacter at high abundances. Microbiome surveys at Santa Cruz Wharf, Monterey Bay, from 2014–2015 showed that Odontella abundance is positively correlated with Flavobacteriaceae and Rhodobacteraceae abundances. Our study demonstrates that specific members of the diatom microbiome can enhance the host’s sexual reproduction, with the interkingdom interaction driven by partner compatibility and long-term association. Sex-enhancing bacteria may even be needed by the diatom host to carry out the optimal inducement of sex under normal conditions, allowing for size restitution and maintaining genetic diversity in culture and in nature.
‘This must be the place’: Understanding video game placeness through atmosphere and the refrain in Dark Souls
Authors: Andiloro Andrea
Published: October 2022

This article explores how atmosphere is implicated in the establishment of place in gameworlds through the concept of the ‘refrain’. The atmosphere of a video game is often one of the most memorable constituents of our ludic experiences and is defining of the video game places we encounter during play, yet atmospheres remain highly under-researched and under-theorized within game studies. The article introduces some key theories of atmosphere and explain their relation to ‘place’ and subsequently explore how ‘place’ has been theorized and developed within game studies. I then elaborate on how atmosphere, understood existentially, is conducive to the creation of an atmosphere by addressing the examples of Dark Souls’ hub area and boss arenas. Working through the Deleuze–Guattarian concept of ‘refrain’, I argue that patterns of sound, images and behaviours establish a territory marked by a certain affective intensity, or atmosphere, enabling the experience of place.
Yugofuturism and subversive mimicry: Ex/Post-Yugoslav Digital (Meme) Culture
Authors: Mevorah Vera
Published: October 2022

The text deals with digital meme culture originating in ex-Yugoslav countries and the forming of a particular post-Yugoslav, (post)digital cultural space which we will examine both in the “affirmative” context of a (yet-to-be-conceived) Yugofuturism, and that of subversive affirmation strategy. This strategy – in which subversion comes not from criticism and clear resistance to any given discourse and action, but from controversial and vivid forms of overemphasis of those same ideologies and imagery – at first glance seems intrinsic to digital meme aesthetics. Following the footsteps of transitions of retrogardism (Neue Slowenische Kunst) and the wider field of contemporary subversive mimicry practices in post-digital art and culture, we will explore this ex/post-Yugoslav shared space in search of a possibility of a(n) (anti)utopic Yugofuturistic discourse.
Some Thoughts on Artistic Re-Imaginings of Yugofuturism’s Utopological Elements
Authors: Majsova Natalija
Published: October 2022

Within the context of societies marked by the postsocialist transition of the 1990s and their cultural – and to a certain extent, their political – practices, we need to highlight the insistence on a meaningful connection between this future and certain past socialist socio-political aspirations as one the more recognizable aspects of thinking about the future. Depending on position or perspective, these aspirations towards equality, interpersonal and intergenerational solidarity, a fairer distribution of resources, and creativity as the basic direction of human life experience may today seem utopian, naïve, or even unnecessary. In this paper, however, I consider whether it is not precisely this legacy of the above-characterised utopianism – not as a teleological orientation towards a predetermined future but rather as a form of action and an attempt to act for the future in the now – that is one of the more meaningful and not at all naïve aspects of the approaches adopted by artistic and cultural practices which we may refer to as yugofuturistic. I will do so by focusing specifically on attitudes towards remembering, and former utopias and related practical issues examined in the works of Marta Popivoda and Ana Vujanović.
There’s No End With No Means
Authors: Dobovšek Zala
Published: October 2022

Based on her doctoral dissertation, Jasna Novakov Sibinović, Serbain theatrologist and editor, wrote Oliver Frljić Political Theatre: From Empathy to Sympathy. In her introduction, she put Frljić’s following statement as her key ideal pointer: “I don’t see theatre as an end but as a means; and I don’t think any less of theatre because of it.” An intentional avoiding of an “end” is, of course, not only pertinent for the topical discussion of theatre or art that should give final answers (and thereby suggest its own inviolability).
Design for circularity through Aesthetic Surgery
Authors: Ravnløkke Louise , Ræbild Ulla
Published: October 2022

This article introduces the term Aesthetic Surgery as a strategy for the designer to contribute to circular economy. Our aim is to discuss this strategy as a way for designers to employ their core competencies to create and innovate the potential of irrelevant materials (waste) through aesthetic means and a material-driven design process. We argue that designers have the skills and experience to seek opportunities in unwanted materials and keep them in a loop of relevance and at a high value. In recent years, mechanical recycling has been gaining footing in the industry. Recycling has, therefore, primarily been concerned with material recovery at fibre level. This can appear to be an easy way to continue business as usual. Yet, this approach is not suitable for all types of materials and material blends and requires further innovation to develop solutions for these situations. Furthermore, these recycling methods use external resources in the process of bringing the material to a point zero and do not utilize the existing parts, components and material qualities. At the same time, sustainable transition requires a break with traditions of large volume productions and fast fashion. Therefore, we argue that we need to broaden the understanding and perspective of recycling and upcycling. The research presented in this article explores fashion and textiles methods of working with aesthetics by proposing Aesthetic Surgery as a material-driven design strategy for recycling and upcycling. Working with aesthetics is well known as a powerful means to create desires and spark imaginations, in this article we suggest turning the attention towards these powerful aesthetic competencies to substantiate the potential of irrelevant (waste) materials. The discussion emerging from this practice-based research offers the potential to further explore the possibilities in design for circularity through an Aesthetic Surgery strategy which may empower designers to contribute to circular economy.
Um exemplo da influência da litologia na estabilidade de taludes
Authors: Oliveira Ricardo
Published: October 2022

A margem direita do rio Tejo, nas proximidades de Santarém, a 70 Km para NE de Lisboa é uma encosta com cerca de 2 Km de comprimento e 80 m de altura.

As formações geológicas são constituídas por intercalações de complexos arenosos e argilo-siltosos sub-horizontais com carácter de estuário.

As inclinações da encosta variam entre 30° e 40° na maior parte da sua extensão. Como consequência, ocorrem todos os anos pequenos escorregamentos e quedas devidos à perda de coesão das formações. Um importante escorregamento ocorreu recentemente numa das extremidades da encosta, envolvendo cerca de 200.000 m3 de terras. Nesta zona a inclinação da encosta era apenas de 20º, pelo que não era de esperar que ocorresse qualquer escorregamento.

O estudo realizado levou à conclusão que o acidente foi devido à presença de um nível de argilas plásticas orgãnicas, intercalado num dos complexos argilo-siltosos, localizado a meia altura da encosta, onde se iniciou um lento processo de fluência que conduziu à rotura. Este nível de argila orgânica apenas ocorre na zona onde se deu o escorregamento.
Túnel 4 (Porto) - análise tridimensional por elementos finitos
Authors: Martins Francisco , Costa Alexandra , Sousa Jorge
Published: October 2022

Neste trabalho apresenta-se os principais resultados da aplicação de modelos de elementos finitos a um túnel superficial escavado em solos residuais graníticos, no Porto. Os cálculos foram efectuados através de análises tridimensionais, nas quais se tentou reproduzir a sequência completa da construção (fase de escavação e aplicação do suporte). O comportamento mecânico dos solos foi reproduzido usando um modelo elástico perfeitamente plástico, tendo os respectivos parâmetros definidores sido estimados com base em resultados de ensaios de campo realizados durante a prospecção e de ensaios laboratoriais. O comportamento observado é comparado com os resultados decorrentes dos cálculos numéricos, tendo-se obtido uma boa concordância.
Archivar la memoria corpográfica: apuestas por la masculinidad sensible en el contexto del cuerpo-archivo
Authors:   Universitat Oberta de Catalunya, Planella Ribera Jordi
Published: October 2022

En este capítulo se analiza el campo de la curación digital con una perspectiva de género. Se realiza un análisis del dominio hasta 2020 haciendo uso, principalmente, de técnicas bibliométricas. Se trabaja con la principal fuente de referencia en el área según consenso, International Journal of Digital Curation (Miguel et al., 2013), y bases de datos internacionales para identificar otras publicaciones que citan esta importante fuente. De estas fuentes se obtuvo una relación exhaustiva de todos los datos de autoría para después identificar el género (masculino o femenino) y trabajar con el concepto de liderazgo a partir del autor de correspondencia.
Curación Digital en la Ciencia de la Información: estrategias del «design» de la Información
Authors:   Universidade Estadual Paulista, Nakano Natalia , Padua Mariana Cantisani ,   Universidade Estadual Paulista, Landim Laís Alpi ,   Universidade Estadual Paulista, Jorente Maria José Vicentini ,   Universidade Estadual Paulista
Published: October 2022

El objetivo de este estudio es presentar el Design de la Información y el Designde Experiencias como estrategias de curación digital para la preservación, el acceso y el intercambio de la información. La metodología empleada es teórico exploratoria, basada en un trecho del ciclo de Curación Digital propuesto por Higgins. El estudio resalta estrategias del Design de la Información y del Design de Experiencias para el perfeccionamiento del acceso y del intercambio de la información proporcionada en entornos informacionales digitales, que, por su vez, favorecen su preservación. El Designde la Información, una vez incorporado a la planificación de curación de entornos digitales, lleva a la eficacia de los procesos comunicacionales en entornos digitales al organizar aspectos materiales, informacionales, sensoriales, cognitivos y humanísticos de los sujetos que interactúan con el entorno.
Perspectiva de género en el entorno digital
Authors:   Universidad Rey Juan Carlos, Blanco-Ruiz Marian
Published: October 2022

Se abordarán los retos que se plantean en el contexto digital y cómo se reproducen los estereotipos, los prejuicios y la discriminación. Una de las manifestaciones de estos sesgos de género es la violencia simbólica a través de las imágenes: ¿cómo se representa a las mujeres en los bancos de imágenes? ¿Qué efectos tienen los estereotipos en la representación que hacen de sí mismos los usuarios de las redes sociales? ¿Qué implicaciones tiene esta representación para el aprendizaje automático? Además, se analizarán los diferentes tipos de violencia en línea que se producen en el entorno de los medios digitales, así como el papel que desempeña la cuestión del anonimato y la suplantación de identidad. Por último, se hará una propuesta para incorporar las perspectivas de género e interseccional en la investigación sobre medios digitales y comunicación.
Toxicity, Genotoxicity and Ecotoxicity of Food Colourants Especially
Synthetic Dyes and its Metabolites: An in silico Approach
Authors: Kargupta Abhijit , Dutta Kakoli , Roychoudhury Dibakar , Rana Subhasis , Talapatra Soumendra Nath
Published: October 2022

In present study, in silico study was performed to predict rat oral acute toxicity, hepatoxicity,
immunotoxicity, genotoxicity endpoints as well as ecotoxicity of daphnids and fish of different food
colourants and its metabolites. A total 13 types of food colourants and 8 metabolites were selected.
The prediction of rat oral acute toxicity (LD50) and genotoxicity endpoints, and ecotoxicity (LC50)
were performed by using ProTox-II webserver and ECOSAR tool. It was obtained as 81 and 275
mg/Kg for tartrazine and green S as class 3 and rests were between class 4-6 for food colourants and 626
and 1925 mg/Kg for aminopyrazolone and 5-sulphoanthranilic acid as class 4 and rest were between 5-6
for metabolites. All the food colourants were non-hepatotoxic while four compounds were found
immuno-toxic. For metabolites, only aminopyrazolone obtained hepatotoxic, but not immuno-toxic.
Allura red was carcinogenic and few were mutagenic, but all were non-cytotoxic. For metabolites,
few were carcinogenic but non-mutagenic and non-cytotoxic.