bluuebunny/crossref_metadata_2025 huggingface dataset:
The roles and applications of short-chain fatty acids derived from microbial fermentation of dietary fibers in human cancer
Authors: Li Yuanqing , Huang Yaxuan , Liang Haili , Wang Wen , Li Bo , Liu Ting , Huang Yuqi , Zhang Zhe , Qin Yutao , Zhou Xiaoying , Wang Rensheng , Huang Tingting
Published: August 2023

Dietary fibers (DFs) and their metabolites attract significant attention in research on health and disease, attributing to their effects on regulating metabolism, proliferation, inflammation, and immunity. When fermented by gut microbiota, DFs mainly produce short-chain fatty acids (SCFAs), such as acetic acid, propionic acid, and butyric acid. As the essential nutrients for intestinal epithelial cells, SCFAs maintain intestinal homeostasis and play essential roles in a wide range of biological functions. SCFAs have been found to inhibit histone deacetylase, activate G protein-coupled receptors, and modulate the immune response, which impacts cancer and anti-cancer treatment. Notably, while extensive studies have illuminated the roles of SCFAs in colorectal cancer development, progression, and treatment outcomes, limited evidence is available for other types of cancers. This restricts our understanding of the complex mechanisms and clinical applications of SCFAs in tumors outside the intestinal tract. In this study, we provide a comprehensive summary of the latest evidence on the roles and mechanisms of SCFAs, with a focus on butyric acid and propionic acid, derived from microbial fermentation of DFs in cancer. Additionally, we recapitulate the clinical applications of SCFAs in cancer treatments and offer our perspectives on the challenges, limitations, and prospects of utilizing SCFAs in cancer research and therapy.
Burden of breast cancer and attributable risk factors in the North Africa and Middle East region, 1990–2019: a systematic analysis for the Global Burden of Disease Study 2019
Authors: Azadnajafabad Sina , Saeedi Moghaddam Sahar , Mohammadi Esmaeil , Rezaei Negar , Rashidi Mohammad-Mahdi , Rezaei Nazila , Mokdad Ali H. , Naghavi Mohsen , Murray Christopher J. L. , Larijani Bagher , Farzadfar Farshad ,   GBD 2019 NAME Breast Cancer Collaborators
Published: August 2023

Background

Breast cancer (BC) is the most common cancer in women globally. The North Africa and Middle East (NAME) region is coping hard with the burden of BC. We aimed to present the latest epidemiology of BC and its risk factors in this region.

Methods

We retrieved the data on BC burden and risk factors from the Global Burden of Disease Study 2019 to describe BC status in the 21 countries of the NAME region from 1990 to 2019. We explored BC incidence, prevalence, deaths, disability-adjusted life years (DALYs), and attributable burden to seven risk factors of female BC, namely, alcohol use, diet high in red meat, low physical activity, smoking, secondhand smoke, high body mass index, and high fasting plasma glucose. Decomposition analysis on BC incidence trend was done to find out the contributing factors to this cancer’s growth.

Results

In 2019, there were 835,576 (95% uncertainty interval: 741,968 to 944,851) female and 10,938 (9,030 to 13,256) male prevalent cases of BC in the NAME region. This number leads to 35,405 (30,676 to 40,571) deaths among female patients and 809 (654 to 1,002) deaths in male patients this year. BC was responsible for 1,222,835 (1,053,073 to 1,411,009) DALYs among female patients in 2019, with a greater proportion (94.9%) of burden in years of life lost (YLLs). The major contributor to female BC incidence increase in the past three decades was found to be increase in age-specific incidence rates of BC (227.5%), compared to population growth (73.8%) and aging (81.8%). The behavioral risk factors were responsible for majority of attributable female BC burden (DALYs: 106,026 [66,614 to 144,247]). High fasting plasma glucose was found to be the risk factor with the largest effect (DALYs: 84,912 [17,377 to 192,838]) on female BC burden.

Conclusion

The increasing incidence and burden of BC in the NAME region is remarkable, especially when considering limited resources in the developing countries of this region. Proper policies like expanding screening programs and careful resource management are needed to effectively manage BC burden.
How we treat octogenarians with brain metastases
Authors: Nieder Carsten , Andratschke Nicolaus H. , Grosu Anca L.
Published: August 2023

Biologically younger, fully independent octogenarians are able to tolerate most oncological treatments. Increasing frailty results in decreasing eligibility for certain treatments, e.g., chemotherapy and surgery. Most brain metastases are not an isolated problem, but part of widespread cancer dissemination, often in combination with compromised performance status. Multidisciplinary assessment is key in this vulnerable patient population where age, frailty, comorbidity and even moderate additional deficits from brain metastases or their treatment may result in immobilization, hospitalization, need for nursing home care, termination of systemic anticancer treatment etc. Here, we provide examples of successful treatment (surgery, radiosurgery, systemic therapy) and best supportive care, and comment on the limitations of prognostic scores, which often were developed in all-comers rather than octogenarians. Despite selection bias in retrospective studies, survival after radiosurgery was more encouraging than after whole-brain radiotherapy. Prospective research with focus on octogenarians is warranted to optimize outcomes.
CCDC43 as a potential therapeutic target of Tian Yang Wan for the treatment of hepatocellular carcinoma by activating the hippo pathway
Authors: Tao Mingyuan , Han Dongwei , Wei Siyu , Gao Changyu
Published: August 2023

Introduction

Hepatocellular carcinoma (HCC) prevalence is rising annually, but the existing treatment strategies are limited; therefore, it is crucial to explore new therapeutic approaches.

Methods

Here, we investigate the potential anti-cancer mechanism of an herbal medicine called Tian Yang Wan (TYW) in the treatment of HCC. The relationship of CCDC43 with immunity and cell death was analyzed by bioinformatics. Confirming the tumor suppressor effect of TYW on HCC cells by proliferation, invasion, migration and apoptosis assays

Results

First, we analyzed by proteomics that CCDC43 expression was downregulated after TYW administration and promoted the hippo pathway. Then, a large sample's transcriptome study demonstrated that elevated CCDC43 expression was strongly correlated with clinical traits and a bad prognosis in HCC patients. Next, we observed through multiple advanced algorithms that CCDC43 is involved in a variety of oncology and immunology related pathways. Notably, we found higher tumor immune microenvironment with high CCDC43 expression. Furthermore, we demonstrated that CCDC43 is associated with immune checkpoints and found that it is a sensitive indicator of a large number of chemotherapeutic agents. Subsequently, we conducted experimental investigations to demonstrate the capacity of TYW to impede proliferation and migration, while inducing apoptosis in human HCC cell lines. Finally, we performed analysis of two cell death patterns which showed CCDC43 to be strongly correlated with multiple ferroptosis factors and cuproptosis factors.

Discusion

In conclusion, our study comprehensively examined the prognostic, immunological, and therapeutic implications of CCDC43 in HCC, thereby elucidating the therapeutic mechanism of action in TYW.
Alleviating effects of coenzyme Q10 supplements on biomarkers of inflammation and oxidative stress: results from an umbrella meta-analysis
Authors: Dabbaghi Varnousfaderani Sara , Musazadeh Vali , Ghalichi Faezeh , Kavyani Zeynab , Razmjouei Soha , Faghfouri Amir Hossein , Ahrabi Sana Sedgh , Seyyed Shoura Seyyed Morteza , Dehghan Parvin
Published: August 2023

Introduction: Although several meta-analyses support the positive effect of coenzyme Q10 (CoQ10) on biomarkers of oxidative stress and inflammation, the results of some other studies reject such effects.

Methods: Therefore, in this umbrella meta-analysis, we performed a comprehensive systematic search in such databases as Web of Science, PubMed, Scopus, Embase, and Google Scholar up to January 2023.

Results: Based on standardized mean difference analysis, CoQ10 supplementation significantly decreased serum C-reactive protein (CRP) (ESSMD = −0.39; 95% CI: 0.77, −0.01, p = 0.042) and malondialdehyde (MDA) (ESSMD = −1.17; 95% CI: 1.55, −0.79, p < 0.001), while it increased the total antioxidant capacity (TAC) (ESSMD = 1.21; 95% CI: 0.61, 1.81, p < 0.001) and serum superoxide dismutase (SOD) activity (ESSMD = 1.08; 95% CI: 0.37, 1.79, p = 0.003). However, CoQ10 supplementation had no significant reducing effect on tumor-necrosis factor-alpha (TNF- α) (ESSMD = −0.70; 95% CI: 2.09, 0.68, p = 0.320) and interleukin-6 (IL-6) levels (ESSMD = −0.85; 95% CI: 1.71, 0.01, p = 0.053). Based on weighted mean difference analysis, CoQ10 supplementation considerably decreased TNF-α (ESWMD = −0.46, 95% CI: 0.65, −0.27; p < 0.001), IL-6 (ESWMD = −0.92, 95% CI: 1.40, −0.45; p < 0.001), and CRP levels (effect sizes WMD = −0.28, 95% CI: 0.47, −0.09; p < 0.001).

Discussion: The results of our meta-analysis supported the alleviating effects of CoQ10 on markers of inflammation cautiously. However, CoQ10 had antioxidant effects regarding the improvement of all the studied antioxidant and oxidative stress biomarkers.

Systematic Review Registration:https://www.crd.york.ac.uk/prospero/display_record.php?RecordID=323861, identifier CRD42022323861
Cyclodextrin nanoparticles in targeted cancer theranostics
Authors: Bose Roshnee , Jayawant Maharsh , Raut Rajesh , Lakkakula Jaya , Roy Arpita , Alghamdi Saad , Qusty Naeem F. , Sharma Rohit , Verma Devvret , Khandaker Mayeen Uddin , Almujally Abdullah , Tamam Nissren , Sulieman Abdelmoneim
Published: August 2023

Graphical Abstract

Cyclodextrin (CD)-based nanotheranostics for cancer treatment.
Cardiovascular safety of Janus kinase inhibitors in patients with rheumatoid arthritis: systematic review and network meta-analysis
Authors: Wei Qige , Wang Hui , Zhao Jianglin , Luo Zhongping , Wang Chufeng , Zhu Chunmei , Su Na , Zhang Shengzhao
Published: August 2023

Background: Janus kinase (JAK) inhibitors have emerged as a progressively utilized therapeutic approach for the management of rheumatoid arthritis (RA). However, the complete determination of their cardiovascular safety remains inconclusive. Hence, the primary objective of this network meta-analysis is to meticulously assess and juxtapose the cardiovascular risks linked to distinct JAK inhibitors employed in RA patients.

Methods: A systematic review and network meta-analysis were meticulously conducted, encompassing a collection of randomized controlled trials (RCTs) that focused on investigating the incidence of major adverse cardiovascular events (MACE) and all-cause mortality associated with Janus kinase (JAK) inhibitors administered to patients with rheumatoid arthritis (RA). Extensive exploration was performed across multiple electronic databases, incorporating studies published until March 2023. To be included in this analysis, the RCTs were required to involve adult participants diagnosed with RA who received treatment with JAK inhibitors. To ensure accuracy, two authors independently undertook the selection of eligible RCTs and meticulously extracted aggregate data. In order to examine the outcomes of MACE and all-cause mortality, a frequentist graph theoretical approach within network meta-analyses was employed, utilizing random-effects models. Third study has been registered on PROSPERO under the reference CRD42022384611.

Findings: A specific selection encompassing a total of 14 meticulously chosen randomized controlled trials was undertaken, wherein 13,524 patients were assigned randomly to distinct treatment interventions. The analysis revealed no notable disparity in the occurrence of major adverse cardiovascular events (MACE) between the interventions and the placebo group. However, in comparison to adalimumab, the employment of JAK inhibitors exhibited an association with higher rates of all-cause mortality [odds ratio (OR): 1.7, 95% confidence interval (CI): 1.02–2.81]. This observed increase in risk primarily stemmed from the usage of tofacitinib (OR: 1.9, 95% CI: 1.12–3.23). None of the other JAK inhibitors exhibited a statistically significant variance in all-cause mortality when compared to adalimumab.

Interpretation: Our study suggests that JAK inhibitors may not increase the risk of MACE in RA patients but may be associated with a higher risk of all-cause mortality compared to adalimumab, primarily due to tofacitinib use. Rheumatologists should carefully consider the cardiovascular risks when prescribing JAK inhibitors, particularly tofacitinib, for RA patients.

Systematic Review Registration:https://www.crd.york.ac.uk/PROSPERO/display_record.php?RecordID=384611, CRD42022384611.
Randomized controlled study to evaluate the safety and clinical impact of percutaneous auricular vagus nerve stimulation in patients with severe COVID-19
Authors: Seitz Tamara , Bergmayr Franziska , Kitzberger Reinhard , Holbik Johannes , Grieb Alexander , Hind Julian , Lucny Felix , Tyercha Alexander , Neuhold Stephanie , Krenn Claus , Wenisch Christoph , Zoufaly Alexander , Kaniusas Eugenijus , Széles József Constantin
Published: August 2023

Introduction: A severe course of COVID-19 is characterized by a hyperinflammatory state resulting in acute respiratory distress syndrome or even multi-organ failure along a derailed sympatho-vagal balance.

Methods: In this prospective, randomized study, we evaluate the hypothesis that percutaneous minimally invasive auricular vagus nerve stimulation (aVNS) is a safe procedure and might reduce the rate of clinical complications in patients with severe course of COVID-19. In our study, patients with SARS-CoV-2 infection admitted to the intensive care unit with moderate-to-severe acute respiratory distress syndrome, however without invasive ventilation yet, were included and following randomization assigned to a group receiving aVNS four times per 24 h for 3 h and a group receiving standard of care (SOC).

Results: A total of 12 patients were included (six in the aVNS and six in the SOC group). No side effects in aVNS were reported, especially no significant pain at device placement or during stimulation at the stimulation site or significant headache or bleeding after or during device placement or lasting skin irritation. There was no significant difference in the aVNS and SOC groups between the length of stay in the intensive care unit and at the hospital, bradycardia, delirium, or 90-day mortality. In the SOC group, five of six patients required invasive mechanical ventilation during their stay at hospital and 60% of them venovenous extracorporeal membrane oxygenation, compared to three of six patients and 0% in the aVNS group (p = 0.545 and p = 0.061).

Discussion: Vagus nerve stimulation in patients with severe COVID-19 is a safe and feasible method. Our data showed a trend to a reduction of progression to the need of invasive ventilation and venovenous extracorporeal membrane oxygenation which encourages further research with larger patient samples.
Transcriptomic analysis of oxidative stress mechanisms induced by acute nanoplastic exposure in Sepia esculenta larvae
Authors: Liu Xiumei , Yang Jianmin , Li Zan
Published: August 2023

Nanoplastics (NPs), as a new type of pollutant with a size small than 1 μm, are ubiquitous and harmful to organisms. There has been an increasing amount of research concerning the effects of NPs on organisms over recent years, especially on aquatic animals. However, there is a limited study on the impact of NPs on mollusk cephalopods. In this research, Sepia esculenta, belonging to Cephalopoda, Coleoidea, Sepioidea, was selected to explore the effects caused by NPs exposure. The S. esculenta larvae were exposed to polystyrene NPs (PS-NPs) with diameter 50 nm (100 mg/L) for 4 h. The detection of oxidative stress biomarkers displayed an obvious increase in SOD (superoxide dismutase) activity and MDA (malondialdehyde) level. Then, RNA-Seq was performed to explore the oxidative stress response at mRNA level. The transcriptome analysis demonstrated that the expression of 2,570 genes was affected by PS-NPs. Besides, the signaling pathways of ribosome, ribosome biogenesis in eukaryotes, proteasome, and MAPK were enriched. This study not only provides novel references for understanding the mechanisms of oxidative stress response induced by NPs, but also reminds us to follow with interest the influence of acute exposure to NPs.
Elucidating the molecular responses to waterlogging stress in onion (Allium cepa L.) leaf by comparative transcriptome profiling
Authors: Gedam Pranjali A. , Khandagale Kiran , Shirsat Dhananjay , Thangasamy A. , Kulkarni Onkar , Kulkarni Abhijeet , Patil Swaranjali S. , Barvkar Vitthal T. , Mahajan Vijay , Gupta Amar Jeet , Bhagat Kiran P. , Khade Yogesh P. , Singh Major , Gawande Suresh
Published: August 2023

Introduction

Waterlogging is a major stress that severely affects onion cultivation worldwide, and developing stress-tolerant varieties could be a valuable measure for overcoming its adverse effects. Gathering information regarding the molecular mechanisms and gene expression patterns of waterlogging-tolerant and sensitive genotypes is an effective method for improving stress tolerance in onions. To date, the waterlogging tolerance-governing molecular mechanism in onions is unknown.

Methods

This study identified the differentially expressed genes (DEGs) through transcriptome analysis in leaf tissue of two onion genotypes (Acc. 1666; tolerant and W-344; sensitive) presenting contrasting responses to waterlogging stress.

Results

Differential gene expression analysis revealed that in Acc. 1666, 1629 and 3271 genes were upregulated and downregulated, respectively. In W-344, 2134 and 1909 genes were upregulated and downregulated, respectively, under waterlogging stress. The proteins coded by these DEGs regulate several key biological processes to overcome waterlogging stress such as phytohormone production, antioxidant enzymes, programmed cell death, and energy production. The clusters of orthologous group pathway analysis revealed that DEGs contributed to the post-translational modification, energy production, and carbohydrate metabolism-related pathways under waterlogging stress. The enzyme assay demonstrated higher activity of antioxidant enzymes in Acc. 1666 than in W-344. The differential expression of waterlogging tolerance related genes, such as those related to antioxidant enzymes, phytohormone biosynthesis, carbohydrate metabolism, and transcriptional factors, suggested that significant fine reprogramming of gene expression occurs in response to waterlogging stress in onion. A few genes such as ADH, PDC, PEP carboxylase, WRKY22, and Respiratory burst oxidase D were exclusively upregulated in Acc. 1666.

Discussion

The molecular information about DEGs identified in the present study would be valuable for improving stress tolerance and for developing waterlogging tolerant onion varieties.
Repeated mechanical damage enhanced Aquilaria sinensis resistance to Heortia vitessoides through jasmonic acid
Authors: Chen Yingying , Liang Shenghua , Wang Shuyao , Li Baocai , Wang Kun , Zhu Yongjin , Yang Risheng , Hao Xin , Yang Zhuoying , Shen Yingbai , Jiang Rihong , Li Kaixiang
Published: August 2023

Introduction

The leaf-chewing pest Heortia vitessoides severely threatens the growth and development of Aquilaria sinensis. In our previous study, we found that mechanical damage (MD) to stem enhanced A. sinensis sapling resistance to H. vitessoides larvae.

Methods

To reveal the defense mechanisms underlying this observation, we analyzed the types and contents of volatile organic compounds (VOCs), phytohormone contents, and expression of phytohormone-related genes in response to MD and herbivory wounding(HW).

Results

Here, we identified several VOCs, such as the pesticides fenobucarb and 2,4-di-tert-butylphenol, in mature leaf (ML) of MD-treated plants. Compared with salicylic acid (SA) or the ethylene (ET) pathway, jasmonic acid (JA) content and JA-related genes were more strongly upregulated. Interestingly, we found a dramatic difference between JA-related upstream and downstream genes expression in YL and ML, which confirmed that JA-Ile accumulation in MD-ML and HW-ML could be derived from local damaged site.

Discussion

Taken together, we provide evidence that the JA pathway plays a dominant role in the A. sinensis response to MD and HW.
An oligosaccharyltransferase from Leishmania donovani increases the N-glycan occupancy on plant-produced IgG1
Authors: Beihammer Gernot , König-Beihammer Julia , Kogelmann Benjamin , Ruocco Valentina , Grünwald-Gruber Clemens , D’Aoust Marc-André , Lavoie Pierre-Olivier , Saxena Pooja , Gach Johannes S. , Steinkellner Herta , Strasser Richard
Published: August 2023

N-Glycosylation of immunoglobulin G1 (IgG1) at the heavy chain Fc domain (Asn297) plays an important role for antibody structure and effector functions. While numerous recombinant IgG1 antibodies have been successfully expressed in plants, they frequently display a considerable amount (up to 50%) of unglycosylated Fc domain. To overcome this limitation, we tested a single-subunit oligosaccharyltransferase from the protozoan Leishmania donovani (LdOST) for its ability to improve IgG1 Fc glycosylation. LdOST fused to a fluorescent protein was transiently expressed in Nicotiana benthamiana and confocal microscopy confirmed the subcellular location at the endoplasmic reticulum. Transient co-expression of LdOST with two different IgG1 antibodies resulted in a significant increase (up to 97%) of Fc glycosylation while leaving the overall N-glycan composition unmodified, as determined by different mass spectrometry approaches. While biochemical and functional features of “glycosylation improved” antibodies remained unchanged, a slight increase in FcγRIIIa binding and thermal stability was observed. Collectively, our results reveal that LdOST expression is suitable to reduce the heterogeneity of plant-produced antibodies and can contribute to improving their stability and effector functions.
The impact of intensive trauma-focused treatment on sexual functioning in individuals with PTSD
Authors: van Woudenberg Clair , Voorendonk Eline M. , Tunissen Bo , van Beek Vince H. F. , Rozendael Linda , Van Minnen Agnes , De Jongh Ad
Published: August 2023

Background

Individuals with posttraumatic stress disorder (PTSD) often experience sexual disturbances.

Objective

To determine whether intensive trauma-focused treatment is associated with an improvement in sexual functioning (i.e., sexual satisfaction and sexual desire) in individuals with PTSD.

Method

In total, 227 patients with PTSD (68.7% women, mean age = 40.97) participated in an intensive eight-day trauma-focused treatment program consisting of prolonged exposure, eye movement and desensitization and reprocessing (EMDR) therapy, physical activity, and psychoeducation. Patients were assessed (i.e., Clinician Administered PTSD Scale and Sexual Functioning Questionnaire) pre- and post-treatment and at 6-months follow-up.

Results

Sexual satisfaction and sexual desire increased significantly associated with trauma-focused treatment from pre-treatment to 6-months follow-up, albeit the effect sizes were small (Cohen’s d = 0.39 and 0.17, respectively). Although men reported greater overall sexual desire than women, sexual functioning improved after treatment in both men and women. Furthermore, those with remission of PTSD reported greater sexual functioning post-treatment and at 6-months follow-up, than those without remission. However, changes in PTSD symptoms associated with treatment were not predictive of the level of sexual satisfaction or sexual desire 6 months after treatment.

Conclusion

The results of this uncontrolled study suggest that intensive treatment for PTSD can have beneficial effects on sexual satisfaction and desire in both men and women; however, this may not necessarily be due to a decrease in PTSD symptoms.
Vulnerability or resilience? Examining trust asymmetry from the perspective of risk sources under descriptive versus experiential decision
Authors: Zhu Jingyuan , Yao Yingying , Jiang Shan
Published: August 2023

Introduction

The investigation of trust vulnerability is important to the understanding of the potential mechanisms of trust formation and erosion. However, more effective exploration of trust vulnerability has been hindered due to the lack of consideration of risk sources and types of information.

Methods

This study extended the investigation of asymmetry to both social and natural risk under experiential and descriptive decisions. Using the trust game as the decision-making paradigm and money as the subject matter, the research employed experimental methods to examine how people perceive and make decisions after being positively and negatively affected by natural and social risks. A total of 286 college students were participated in our study. Study 1 (n = 138) and Study 2 (n = 148) explored asymmetry in experiential and descriptive decision separately.

Results

The findings indicated that when considering experiential information, negative information had a greater effect in reducing trust compared to the enhancing effect of positive information (t = −1.95, p = 0.050). Moreover, the study revealed that negative information had a stronger negative impact in the context of social risks rather than natural risks (t = −3.26, p = 0.002), suggesting that trust is vulnerable both internally and externally. Conversely, when considering descriptive information, the effect of both positive and negative information on trust was symmetrical, and the impact of negative information was less significant compared to that of natural risks, indicating that trust has a certain level of resilience (t = 2.25, p = 0.028).

Discussion

The study emphasizes the importance of refining risk sources and information characteristics in complex scenarios in order to improve understanding of trust enhancement and repair.
The effect of bio-electro-magnetic-energy-regulation therapy on sleep duration and sleep quality among elite players in Norwegian women’s football
Authors: Moen Frode , Pettersen Svein Arne , Gjertsås Kine , Vatn Marte , Ravenhorst Martijn , Kvålsvoll Atle , Liland Kristian Hovde , Mosleth Ellen F.
Published: August 2023

The current study investigated if physical loads peak on game days and if Bio-Electro-Magnetic-Energy-Regulation (BEMER) therapy is affecting sleep duration and sleep quality on nights related to game nights among elite players in Norwegian women’s elite football. The sample included 21 female football players from an elite top series club with a mean age of ~24 years (± 2.8). Sleep was measured every day over a period of 273 consecutive days with a Somnofy sleep monitor based on ultra-wideband (IR-UWB) pulse radar and Doppler technology. The current study was conducted as a quasi-experiment, where each player was their own control based on a control period that lasted for 3 months, and an experimental period that lasted for 5 months. Accordantly, the time each player spent with BEMER therapy was used as a control variable. Multivariate analyses of variance using FFMANOVA and univariate ANOVA with False Discovery Rate adjusted p-values show that physical performance (total distance, distance per minute, sprint meters >22.5 kmh, accelerations and decelerations) significantly peak on game day compared with ordinary training days and days related to game days. The results also show that sleep quantity and quality are significantly reduced on game night, which indicate disturbed sleep caused by the peak in physical load. Most sleep variables significantly increased in the experiment period, where BEMER therapy was used, compared to the control period before the introduction of BEMER therapy. Further, the analyses show that players who spent BEMER therapy >440 h had the most positive effects on their sleep, and that these effects were significantly compared to the players who used BEMER therapy <440 h. The findings are discussed based on the function of sleep and the different sleep stages have on recovery.
Beyond adaptive cruise control and lane centering control: drivers’ mental model of and trust in emerging ADAS technologies
Authors: Huang Chunxi , He Dengbo , Wen Xiao , Yan Song
Published: August 2023

Introduction

The potential safety benefits of advanced driver assistance systems (ADAS) highly rely on drivers’ appropriate mental models of and trust in ADAS. Current research mainly focused on drivers’ mental model of adaptive cruise control (ACC) and lane centering control (LCC), but rarely investigated drivers’ understanding of emerging driving automation functions beyond ACC and LCC.

Methods

To address this research gap, 287 valid responses from ADAS users in the Chinese market, were collected in a survey study targeted toward state-of-the-art ADAS (e.g., autopilot in Tesla). Through cluster analysis, drivers were clustered into four groups based on their knowledge of traditional ACC and LCC functions, knowledge of functions beyond ACC and LCC, and knowledge of ADAS limitations. Predictors of driver grouping were analyzed, and we further modeled drivers’ trust in ADAS.

Results

Drivers in general had weak knowledge of LCC functions and functions beyond ACC and LCC, and only 27 (9%) of respondents had a relatively strong mental model of ACC and LCC. At the same time, years of licensure, weekly driving distance, ADAS familiarity, driving style (i.e., planning), and personability (i.e., agreeableness) were associated with drivers’ mental model of ADAS. Further, it was found that the mental model of ADAS, vehicle brand, and drivers’ age, ADAS experience, driving style (i.e., focus), and personality (i.e., emotional stability) were significant predictors of drivers’ trust in ADAS.

Discussion

These findings provide valuable insights for the design of driver education and training programs to improve driving safety with ADAS.
The cognitive effects of adjunctive repetitive transcranial magnetic stimulation for late-onset depression: a randomized controlled trial with 4 week follow-up
Authors: Pan Wei-gang , Hu Xiao-yue , Zhu Dan-di , Li Li , Bao Feng , Ren Li , Mao Pei-xian , Ma Xin , Ren Yan-ping , Tang Yi-lang
Published: August 2023

Objectives

Cognitive impairment is common and linked to poor outcomes in patients with late-onset depression (LOD). The cognitive effects of repetitive transcranial magnetic stimulation (rTMS) for LOD are not well understood. This study aimed to investigate the effects of rTMS on cognitive function in elderly patients with LOD.

Methods

In total, 58 elderly patients (aged 60 to 75 years) with depression were enrolled and randomly assigned to an active rTMS group or a sham group. The participants received active or sham rTMS over the left dorsolateral prefrontal cortex for 4 weeks, 5 days a week, at a frequency of 10 Hz rTMS and 120% of the motor threshold (MT). Cognitive function was assessed using the Repeatable Battery for the Assessment of Neuropsychological Status (RBANS) at baseline, the end of the 4 week treatment period, and at the 4 week follow-up.

Results

The active rTMS group showed significant improvements in immediate memory and attention scores on the RBANS compared to the sham group. However, no significant differences were observed between the two groups in other cognitive domains assessed by the RBANS. No serious adverse events related to rTMS treatment were observed.

Conclusion

Treatment with 120% MT rTMS was associated with improvement in cognitive defects related to the active phase of LOD. These findings suggest that rTMS could provide early improvements in cognitive function in clinical settings for elderly patients with LOD.

Clinical trial registration: https://www.chictr.org.cn/showproj.html?proj=40698, identifier ChiCTR1900024445.
Preoperative prediction of lymphovascular invasion of colorectal cancer by radiomics based on 18F-FDG PET-CT and clinical factors
Authors: Yang Yan , Wei Huanhuan , Fu Fangfang , Wei Wei , Wu Yaping , Bai Yan , Li Qing , Wang Meiyun
Published: August 2023

Purpose

The purpose of this study was to investigate the value of a clinical radiomics model based on Positron emission tomography-computed tomography (PET-CT) radiomics features combined with clinical predictors of Lymphovascular invasion (LVI) in predicting preoperative LVI in patients with colorectal cancer (CRC).

Methods

A total of 95 CRC patients who underwent preoperative 18F-fluorodeoxyglucose (FDG) PET-CT examination were retrospectively enrolled. Univariate and multivariate logistic regression analyses were used to analyse clinical factors and PET metabolic data in the LVI-positive and LVI-negative groups to identify independent predictors of LVI. We constructed four prediction models based on radiomics features and clinical data to predict LVI status. The predictive efficacy of different models was evaluated according to the receiver operating characteristic curve. Then, the nomogram of the best model was constructed, and its performance was evaluated using calibration and clinical decision curves.

Results

Mean standardized uptake value (SUVmean), maximum tumour diameter and lymph node metastasis were independent predictors of LVI in CRC patients (P < 0.05). The clinical radiomics model obtained the best prediction performance, with an Area Under Curve (AUC) of 0.922 (95%CI 0.820–0.977) and 0.918 (95%CI 0.782–0.982) in the training and validation cohorts, respectively. A nomogram based on the clinical radiomics model was constructed, and the calibration curve fitted well (P > 0.05).

Conclusion

The clinical radiomics prediction model constructed in this study has high value in the preoperative individualized prediction of LVI in CRC patients.
Achieving global surgical excellence: an evidence-based framework to guide surgical quality improvement programs in low and middle income countries
Authors: Henry Jaymie Claire , Wong Lye-Yeng , Reyes Ana M. , Jin James Z. , Ferguson Mark K. , Yip Cheng Har , Hill Andrew
Published: August 2023

Objectives

There is a lack of evidence-based guidelines for enhancing global surgical care delivery. We propose a set of recommendations to serve as a framework to guide surgical quality improvement and scale-up initiatives in low and middle income countries (LMICs).

Methods

From January-December 2019, we reviewed the available literature and their application toward LMIC settings. The first initiative was the establishment of Best Practices Recommendations intended to summarize best-level evidence around quality improvement processes that have shown to decrease morbidity and mortality in LMICs. The GRADE level of evidence and strength of the recommendation were assigned in accordance with the WHO handbook for guidelines development. The second initiative was the scale-up of principles and practices by establishing international expert consensus on the optimal organization of surgical services in LMICs using a modified Delphi methodology.

Results

Recommendations for three topic areas were established: reducing surgical site infections, improving quality of trauma systems, and interventions to reduce maternal and perinatal mortality. 27 studies were included in a quantitative synthesis and meta-analysis for interventions reducing surgical site infections, 27 studies for interventions improving the quality of trauma systems, and 14 studies for interventions reducing maternal and perinatal mortality. Using Delphi methodology, an international expert panel established consensus that district hospitals should place the highest priority on developing surgical services for low complexity, high volume conditions. At the national level, emergency and essential surgical care should be integrated within national Universal Health Coverage frameworks.

Conclusions

This project fills a critical cap in the rapidly developing field of global surgery: gathering evidence-based, practical, and cost-effective solutions that will serve as a guide for the efficient planning and allocation of resources necessary to promote quality and safe essential surgical services in LMICs.
Device-based physical activity measures for population surveillance—issues of selection bias and reactivity
Authors: Christiansen Lars Breum , Koch Sofie , Bauman Adrian , Toftager Mette , Bjørk Petersen Christina , Schipperijn Jasper
Published: August 2023

Background

Device-based measurement in physical activity surveillance is increasing, but research design choices could increase the risk of self-selection bias and reactive behaviour. The aim of this study is to compare the self-reported physical activity profiles of four different samples: participants in a large national survey, participants in a telephone-based survey of non-responders, participants in the large national survey who accepted the invitation to device-based measuring, and the same sample during the week of monitoring.

Methods

In October 2020, 163,133 Danish adults participated in a national survey and of those 39,480 signed up for device-based measurements. A balanced random sample (n = 3,750) was invited to wear an accelerometer of whom 1,525 accepted the invitation. Additionally, a short telephone-based survey on 829 non-responders to the national survey was conducted. Sociodemographic characteristics and self-reported weekly frequencies of physical activity across four domains are compared.

Results

The participants in the national survey were older, more often female, and more often not working. Participants in the telephone-based survey were younger, more often doing unskilled work, and were more often active at home and at work. The participants in the device-based sample were more often active during transport and leisure in the national survey, and participants categorized in the most active category increased during the week of monitoring from 29.0% to 60.7% and from 58.5% to 81.7% for active transport and leisure activities, respectively.

Conclusion

Recruiting a population representative sample for device-based measurement of physical activity is challenging, and there is a substantial risk of sample selection bias and measurement reactivity. Further research in this area is needed if device-based measures should be considered for population physical activity surveillance.
The nutritional composition of Australian native grains used by First Nations people and their re-emergence for human health and sustainable food systems
Authors: Birch Jacob , Benkendorff Kirsten , Liu Lei , Luke Hanabeth
Published: August 2023

First Nations people of Australia sustained complex grassland grain production systems prior to colonisation. The revival of these foodways could aid in mitigating the interlinked issues of land degradation, reduced landscape resilience and declining food security. For the Gamilaraay people, original custodians of the grasslands of north-west New South Wales and south-west Queensland, efforts are underway to bring their ancient food system into a modern context with authenticity and integrity. The aim of this transdisciplinary study was to investigate the nutritional quality of Australian native grains to identify functional properties that may help promote this nascent industry; complimented by using autoethnography to understand how the original custodians, like the Gamilaraay people, might equitably benefit. Ethnographic findings highlight that Gamilaraay people aspire to improve their health and wellbeing through economic development and consumption of native grains, particularly Elders who disproportionately suffer from non-communicable disease. However, many First Nations people have lived experiences of being systemically exploited and excluded, particularly in the food and agriculture space. To prioritise the interests of the Traditional Custodians, the species used in the biochemical assays were de-identified. Wholegrains from seven culturally significant species, with domesticated brown rice as control, were threshed, milled, and analysed in triplicate for proximate, elemental, non-starch fatty acids, and total free phenolic content. Compared to brown rice, protein was significantly higher in all native species (9.4–32.6 g/100 g); whilst carbohydrates were significantly lower (36.5–53.7 g/100 g). One of the native species had exceptionally high total phenolics (569 mg GAE/100 g) compared to brown rice (60 mg GAE/100 g). All native species had generally higher elemental content, with significantly higher levels of Ca, Fe, Zn, Cu, Mg, P, and K in two native species. All samples were dominated by unsaturated fats with significantly higher polyunsaturated fats in two native species. The generally low carbohydrates, high protein, good fats, high mineral content and high phenolic content suggests that native grains may be beneficial to human health by improving nutrient intake and protecting against non-communicable disease, thus marketable as functional foods. To ensure a flourishing industry where all of Australia benefits from these healthful grains, future research and industry development must be First Nations led.
Quantification of spinal ataxia in dogs with thoracolumbar spinal cord injury
Authors: Sherif Tamara , Twele Friederike , Meller Sebastian , Müller-Anders Alexandra , Volk Holger A.
Published: August 2023

The clinical sign of ataxia is related to several neurological diseases and is seen in conjunction with paresis in dogs with spinal cord injury (SCI). Endeavours to objectify canine spinal ataxia in SCI remain limited. The aim of this clinical study was to determine and quantify differences between gait characteristics of ataxic dogs with thoracolumbar myelopathy and healthy control dogs using a computer-and treadmill-based gait analysis system. Five dogs with spinal ataxia and six healthy dogs underwent video-and computer-assisted gait analysis while walking on a four-ground reaction force plate treadmill system (maximum speed of 0.7 m/s). Spatio-temporal and kinetic gait characteristics regarding the dogs’ locomotion were analysed with a focus on the individual coefficient of variation (CV), as a potential measure for quantification of the level of ataxia. Ataxic dogs with thoracolumbar SCI showed no effect on symmetry indices but higher variability in spatio-temporal and kinetic gait parameters mainly in the pelvic, but also in the thoracic limbs. Double support phase of the individual limb was prolonged in SCI dogs at the cost of the single support and swing phase. Reduced peaks of ground reaction forces (GRF) could potentially be explained by reduction of muscle strength, as a strategy of avoiding falling by taking enthusiastic steps, or by alteration of the rhythmogenic spinal circuits between the pelvic and thoracic limb pattern generators.
Characteristics, surgical treatment, and outcomes of injuries involving the tarsus in greyhounds
Authors: Biggo Morgan R. , Jones Stephen C. , Wanstrath Audrey W. , Tinga Selena , Dyce Jonathan , Carson Brittney A. , Schaul Kelsey , Follette Christelle M. , Kieves Nina R.
Published: August 2023

Objective

The first objective of this study was to describe the type of tarsal injuries sustained, surgery performed, and postoperative complications in greyhounds presenting to a single veterinary hospital. An additional objective of the study was to determine the surgical site infection (SSI) and explantation rate, and if any variables were associated with an increased risk of SSI and/or explantation.

Animals

116 greyhounds receiving surgical intervention for a tarsal injury.

Proceures

Medical records from a single veterinary referral hospital were reviewed retrospectively. Data retrieved included signalment, details regarding the injury, surgical intervention, concurrent castration, surgical/anesthesia times, postoperative management, time to healing, and postoperative complications. In cases that underwent explantation, cause, time from initial surgery, and risk factors were evaluated.

Results

The most frequently diagnosed tarsal injuries were fracture of the central tarsal bone (CTB; 57.8%), calcaneal fracture (56.9%) and proximal intertarsal subluxation (34.5%). The most common injury combination was a CTB fracture with a calcaneal fracture (31.9%). In total 115 (99.1%) survived to discharge. Of these, 46 (40.0%) were diagnosed with an SSI and 59 (51.3%) underwent explantation. The most common indication for explantation was SSI. Concurrent medial and lateral surgical approaches was found to be associated with an increased likelihood of SSI and explantation.

Clinical relevance

Practitioners performing surgical intervention for tarsal injuries in greyhounds should be aware of the high SSI rate and likelihood that explantation will be required. This risk is elevated for injuries requiring a bilateral surgical approach.
The Environmental Tax Scheme in China’s Large-Scale Pig Farming: Balancing Economic Burden and Responsibility
Authors: Yan Tiemei , Zhang Tong , Zhu Zhanguo
Published: August 2023

China has implemented an environmental protection tax for large-scale agricultural farming to address environmental pollution caused by livestock and poultry breeding. Studying the environmental management of large-scale pig farming is crucial for controlling agricultural pollution. However, the economic impact and effectiveness of the current tax design, including responsibility sharing, tax rates, and taxpayers, are unclear. This study aims to address these gaps. Firstly, two types of taxation principles, production-based and consumption-based, are established, and their effects on pollution emissions distribution between pig production and marketing areas are comparatively analyzed. Secondly, the economic impact of consumption-based environmental taxes is estimated from inter-provincial and rural–urban perspectives under the current tax mechanism. Thirdly, a new alternative tax rate aligned with inter-provincial pig consumption levels is proposed based on the consumption principle. By comparing alternative tax systems, the potential impact of the current environmental tax on the regional economic burden is analyzed. The results highlighted that a production-based tax system resulted in 83% of regions experiencing varying degrees of implicit emission transfers, and a consumption-based tax system helped coordinate the environmental economic burden between pig production and marketing regions. Additionally, a linear relationship between tax rates and pork consumption expenditure not only alleviated the overall economic burden, leading to tax savings in 30% of regions, but also increased the national environmental revenue from the pig farming industry, resulting in a remarkable 147% rise in overall environmental tax revenue. These findings provide theoretical support for adjusting responsibility and economic burden through environmental tax modifications, facilitating the establishment of a compensation mechanism for the benefits between pig production and marketing areas.
Growth–Immunity Tradeoff in Brassica Crops
Authors: Vega-Álvarez Carmen , Francisco Marta , Soengas Pilar
Published: August 2023

In recent decades, the research community evidenced that plants affected by pathogens and pests may display delayed development [...]