bluuebunny/crossref_metadata_2025 huggingface dataset:
Transmission Line Object Detection Method Based on Label Adaptive Allocation
Authors: Zhao Lijuan , Liu Chang’an , Zhang Zheng , Qu Hongquan
Published: June 2022

Inspection of the integrality of components and connecting parts is an important task to maintain safe and stable operation of transmission lines. In view of the fact that the scale difference of the auxiliary component in a connecting part is large and the background environment of the object is complex, a one-stage object detection method based on the enhanced real feature information and the label adaptive allocation is proposed in this study. Based on the anchor-free detection algorithm FCOS, this method is optimized by expanding the real feature information of the adjacent feature layer fusion and the semantic information of the deep feature layer, as well as adaptively assigning the label through the idea of pixel-by-pixel detection. In addition, the grading ring image is sliced in original data to improve the proportion of bolts in the dataset, which can clear the appearance features of small objects and reduce the difficulty of detection. Experimental results show that this method can eliminate the background interference in the GT (ground truth) as much as possible in object detection process, and improve the detection accuracy for objects with a narrow shape and small size. The evaluation index AP (average precision) increased by 4.1%. Further improvement of detection accuracy lays a foundation for the realization of efficient real-time patrol inspection.
Exploration of Darcy–Forchheimer Flows of Non-Newtonian Casson and Williamson Conveying Tiny Particles Experiencing Binary Chemical Reaction and Thermal Radiation: Comparative Analysis
Authors: Eswaramoorthi Sheniyappan , Thamaraiselvi S. , Loganathan Karuppusamy
Published: June 2022

This discussion intends to scrutinize the Darcy–Forchheimer flow of Casson–Williamson nanofluid in a stretching surface with non-linear thermal radiation, suction and heat consumption. In addition, this investigation assimilates the influence of the Brownian motion, thermophoresis, activation energy and binary chemical reaction effects. Cattaneo–Christov heat-mass flux theory is used to frame the energy and nanoparticle concentration equations. The suitable transformation is used to remodel the governing PDE model into an ODE model. The remodeled flow problems are numerically solved via the BVP4C scheme. The effects of various material characteristics on nanofluid velocity, nanofluid temperature and nanofluid concentration, as well as connected engineering aspects such as drag force, heat, and mass transfer gradients, are also calculated and displayed through tables, charts and figures. It is noticed that the nanofluid velocity upsurges when improving the quantity of Richardson number, and it downfalls for larger magnitudes of magnetic field and porosity parameters. The nanofluid temperature grows when enhancing the radiation parameter and Eckert number. The nanoparticle concentration upgrades for larger values of activation energy parameter while it slumps against the reaction rate parameter. The surface shear stress for the Williamson nanofluid is greater than the Casson nanofluid. There are more heat transfer gradient losses the greater the heat generation/absorption parameter and Eckert number. In addition, the local Sherwood number grows when strengthening the Forchheimer number and fitted rate parameter.
Determinants of Place of Delivery during the COVID-19 Pandemic—Internet Survey in Polish Pregnant Women
Authors: Strózik Mateusz , Szarpak Lukasz , Adam Ishag , Smereka Jacek
Published: June 2022

Background and Objectives: COVID-19 is a pandemic disease, and its unpredictable outcome makes it particularly dangerous, especially for pregnant women. One of the decisions they have to make is where they will give birth. This study aimed to determine the factors influencing the choice of place of delivery and the impact of the COVID 19 pandemic on these factors. Materials and Methods: The study was conducted on 517 respondents from Poland. The research methods comprised the authors’ own survey questionnaire distributed via the Internet from 8 to 23 June 2021. The survey was fully anonymous, voluntary, and addressed to women who gave birth during the pandemic or will give birth shortly. Results: A total of 440 (85.1%) respondents were afraid of SARS-CoV-2 infection. The most frequently indicated factors were fear of complications in the newborn, fear of intrauterine fetal death, and congenital disabilities in a newborn. A total of 74 (14.3%) women considered home delivery. The main factors that discouraged the choice of home birth were the lack of professional medical care 73.1% (N = 378), the lack of anesthesia 23.6% (N = 122), and the presence of indications for caesarean section 23.4% (N = 121). The possibility of mother–child isolation caused the greatest fear about hospital delivery. During the COVID-19 pandemic, pregnant women concerned about SARS-CoV-2 infection were more likely to consider home delivery than those without such fears. The most important factors affecting the choice of the place of delivery included the possibility of a partner’s presence, excellent sanitary conditions and optimal distance from the hospital, and the availability of epidural analgesia for delivery. Conclusions: Our study identifies the determinants of place of delivery during the COVID-19 pandemic. The data we obtained can result in the healthcare system considering patients’ needs in case of similar crisis in the future.
Robotic versus Laparoscopic Gastrectomy for Gastric Cancer: An Updated Systematic Review
Authors: Zizzo Maurizio , Zanelli Magda , Sanguedolce Francesca , Torricelli Federica , Morini Andrea , Tumiati David , Mereu Federica , Zuliani Antonia Lavinia , Palicelli Andrea , Ascani Stefano , Giunta Alessandro
Published: June 2022

Background and Objectives: Gastrectomy with D2 lymphadenectomy is the standard surgical treatment with curative intent for patients with gastric cancer (GC). Over the last three decades, surgeons have been increasingly adopting laparoscopic surgery for GC, due to its better short-term outcomes. In particular, laparoscopic gastrectomy (LG) has been routinely used for early gastric cancer (EGC) treatment. However, LG suffers from technical limitations and drawbacks, such as a two-dimensional surgical field of view, limited movement of laparoscopic tools, unavoidable physiological tremors and discomfort for operating surgeon. Therefore, robotic surgery has been developed to address such limitations. Materials and Methods: We performed a systematic review following the Preferred Reporting Items for Systematic Reviews and Meta-Analyzes (PRISMA) guidelines in order to investigate the benefits and harms of robotic gastrectomy (RG) compared to the LG. PubMed/MEDLINE, Scopus, Cochrane Library (Cochrane Database of Systematic Re-views, Cochrane Central Register of Controlled Trials-CENTRAL) and Web of Science (Science and Social Science Citation Index) databases were used to search all related literature. Results: The 7 included meta-analyses covered an approximately 20 years-study period (2000–2020). Almost all studies included in the meta-analyses were retrospective ones and originated from Asian countries (China and Korea, in particular). Examined overall population ranged from 3176 to 17,712 patients. If compared to LG, RG showed both operative advantages (operative time, estimated blood loss, number of retrieved lymph nodes) and perioperative ones (time to first flatus, time to restart oral intake, length of hospitalization, overall complications, Clavien-Dindo (CD) ≥ III complications, pancreatic complications), in the absence of clear differences of oncological outcomes. However, costs of robotic approach appear significant. Conclusions: It is impossible to make strong recommendations, due to the statistical weakness of the included studies. Further randomized, possibly multicenter trials are strongly recommended, if we want to have our results confirmed.
Role of Interleukin-6 in the Antigen-Specific Mucosal Immunoglobulin A Responses Induced by CpG Oligodeoxynucleotide-Loaded Cationic Liposomes
Authors: Tada Rui , Honjo Emi , Muto Shoko , Takayama Noriko , Kiyono Hiroshi , Kunisawa Jun , Negishi Yoichi
Published: June 2022

An advantage of mucosal vaccines over conventional parenteral vaccines is that they can induce protective immune responses not only at mucosal surfaces but also in systemic compartments. Despite this advantage, few live attenuated or inactivated mucosal vaccines have been developed and applied clinically. We recently showed that the intranasal immunization of ovalbumin (OVA) with class B synthetic oligodeoxynucleotides (ODNs) containing immunostimulatory CpG motif (CpG ODN)-loaded cationic liposomes synergistically exerted both antigen-specific mucosal immunoglobulin A (IgA) and systemic immunoglobulin G (IgG) responses in mice. However, the mechanism underlying the mucosal adjuvant activity of CpG ODN-loaded liposomes remains unknown. In the present study, we showed that the intranasal administration of CpG ODN-loaded cationic liposomes elicited interleukin (IL)-6 release in nasal tissues. Additionally, pre-treatment with an anti-IL-6 receptor (IL-6R) antibody attenuated antigen-specific nasal IgA production but not serum IgG responses. Furthermore, the intranasal administration of OVA and CpG ODN-loaded cationic liposomes increased the number of IgA+/CD138+ plasma cells and IgA+/B220+ B cells in the nasal passages. This increase was markedly suppressed by pre-treatment with anti-IL-6R blocking antibody. In conclusion, IL-6 released by CpG ODN-loaded cationic liposomes at the site of administration may play a role in the induction of antigen-specific IgA responses by promoting differentiation into IgA+ plasma cells for IgA secretion from B cells.
Doped Potassium Jarosite: Synthesis, Characterization and Evaluation as Biomaterial for Its Application in Bone Tissue Engineering
Authors: Serralde-Lealba Juan R. , Cerecedo-Sáenz Eduardo , Hernández-Ávila Juan , Arenas-Flores Alberto , Veloz-Rodríguez María A. , Gutiérrez-Amador María del P. , González-González Arely M. , Rosales-Ibáñez Raúl , Salinas-Rodríguez Eleazar
Published: June 2022

For decades, jarosites have been precipitated by controlling Fe in hydrometallurgical circuits. In addition, their synthesis, characterization, precious metals incorporation, decomposition and leaching have led to important results in this field. Nowadays, new topics related to the synthesis of these compounds have directed studies for applications such as lithium-ion batteries (as cathodes or/and anodes). Additionally, in this work, the evaluation of these kinds of compounds as biomaterials to be used in bone tissue engineering is shown, which is a novel application of these jarosite type-compounds. The method used for the synthesis of these compounds has been improved, decreasing the temperature (from 95 to 70 °C) and synthesis time (from 24 to only 3 h), which allows the doping of the potassium jarosite with calcium, strontium and magnesium (JKCa, JKCa2 and JKAll). The powders obtained this way were characterized confirming the incorporation of these elements into the structure, and the biological assays allowing the cell proliferation at 10 days conclude that these compounds are viable as a biomaterial, due to their non-toxic property. On the other hand, these jarosites show osteoinduction when added to the swine dental pulp stem cells and can be used for orthodontic purpouses.
Solvent Extraction for Separation of 99.9% Pure Cobalt and Recovery of Li, Ni, Fe, Cu, Al from Spent LIBs
Authors: Meshram Pratima , Virolainen Sami , Abhilash Abhilash , Sainio Tuomo
Published: June 2022

In this work, hydrometallurgical recycling of metals from high-cobalt-content spent lithium-ion batteries (LIBs) from laptops was studied using precipitation and solvent extraction as alternative purification processes. Large amounts of cobalt (58% by weight), along with nickel (6.2%), manganese (3.06%) and lithium (6.09%) are present in LiCoO2 and Li2CoMn3O8 as prominent Co-rich phases of the electrode material. The pregnant leach solution (PLS) that was generated by leaching in the presence of 10% H2O2 using 50 g/L pulp density at 80 °C for 4 h contained 27.4 g/L Co, 3.21 g/L Ni, 1.59 g/L Mn and 3.60 g/L Li. The PLS was subjected to precipitation at various pH using 2 M NaOH but the purification performance was poor. To improve the separation of Mn and other impurities and in order to avoid the loss of cobalt and nickel, separation studies were carried out using a solvent extraction technique using di-(2-ethylhexyl) phosphoric acid (D2EHPA) and bis-(2,4,4-trimethylpentyl) phosphinic acid (Cyanex 272). Overall, this study examines the fundamentals of separating and synthesizing 99.9% pure Co sulfate from leach liquor of spent laptop LIBs with remarkably high cobalt content.
Application of Dual Silane Coupling Agent-Assisted Surface-Modified Quartz Powder in Epoxy Matrix for Performance Enhancement
Authors: Li Peiyue , Ma Liyun , Ren Zijie , Xie Enjun , Wang Zengzi , Xie Liusha , Gao Huimin , Zhou Xinjun , Wu Jianxin
Published: June 2022

Quartz powder (QP) is an inorganic filler that is expected to significantly enhance the dielectric and mechanical properties of epoxy (EP)-based composites applied in copper clad laminates and epoxy molding compounds for 5G applications. As is well-known, the performance of the QP–EP composites is directly correlated with the dispersion effect and the compatibility of QP with an EP matrix. Herein, we propose the surface modification method of QP by mixing SCAs of different alkyl chain lengths that contain amino and carbonyl groups. Different characterization methods (FTIR, TGA, XPS, SEM, contact angle measurement, viscosity, and mechanical properties) and molecular dynamics simulation were adopted to study its effect and mechanism. Through dual SCA modification, the viscosity of QP–EP composites was reduced by 11.70%, and the flexural and tensile strengths increased by 16.89% and 30.01%, respectively. In addition, it was revealed that the superiority of dual SCAs originated from the synergistic effect between APTES and SPIS, it was the electrostatic repulsion force between the amino groups of the two SCAs that caused a steric hindrance that activated the steric stabilization effect of SPIS, thus resulting in better dispersion and excellent compatibility. Meanwhile, the amino and carbonyl groups of the SCA interacted with the EP matrix via chemical bonds and hydrogen bonds, thus strengthening the interfacial adhesion between the QP and EP matrix and improving the mechanical performance of QP–EP composites. These results are evidence of the potential of the proposed approach, which is based on the synergistic compounding of SCA with different molecular structures, in powder industrial applications.
UV-Based Advanced Oxidation Processes of Remazol Brilliant Blue R Dye Catalyzed by Carbon Dots
Authors: Cardoso Inês M. F. , Cardoso Rita M. F. , Pinto da Silva Luís , Esteves da Silva Joaquim C. G.
Published: June 2022

UV-based advanced oxidation processes (AOPs) (UV/H2O2 and UV/S2O82−) with a titanium(IV)-doped carbon dot, TiP-CD, as a catalyst were developed for the decomposition of Remazol Brilliant Blue R (Reactive Blue 19), an anthraquinone textile dye (at T = 25 °C and pH = 7). The Ti-CD, with marked catalytic UV properties, was successfully synthesized by the one-pot hydrothermal procedure, using L-cysteine as carbon precursor, ethylenediamine as nitrogen source, PEG (polyethylene glycol) as a capping agent, and titanium(IV) isopropoxide (precursor of TiO2 doping). Contrary to azo dyes (methyl orange, orange II sodium salt, and reactive black 5), which achieved complete degradation in a time interval less than 30 min in the developed AOP systems (UV/H2O2, UV/S2O82−, and UV/TiO2), the RBB-R showed relatively low degradation rates and low discoloration rate constants. In the presence of the catalyzer, the reaction rate significantly increased, and the pseudo-first-order rate constants for the RBB-R discoloration were UV/3.0 mM H2O2/TIP-CD-0.0330 min−1 and UV/1.02 mM S2O82−/TIP-CD-0.0345 min−1.
Microwave-Assisted Synthesis of Zn2SnO4 Nanostructures for Photodegradation of Rhodamine B under UV and Sunlight
Authors: Rovisco Ana , Morais Maria , Branquinho Rita , Fortunato Elvira , Martins Rodrigo , Barquinha Pedro
Published: June 2022

The contamination of water resources by pollutants resulting from human activities represents a major concern nowadays. One promising alternative to solve this problem is the photocatalytic process, which has demonstrated very promising and efficient results. Oxide nanostructures are interesting alternatives for these applications since they present wide band gaps and high surface areas. Among the photocatalytic oxide nanostructures, zinc tin oxide (ZTO) presents itself as an eco-friendly alternative since its composition includes abundant and non-toxic zinc and tin, instead of critical elements. Moreover, ZTO nanostructures have a multiplicity of structures and morphologies possible to be obtained through low-cost solution-based syntheses. In this context, the current work presents an optimization of ZTO nanostructures (polyhedrons, nanoplates, and nanoparticles) obtained by microwave irradiation-assisted hydrothermal synthesis, toward photocatalytic applications. The nanostructures’ photocatalytic activity in the degradation of rhodamine B under both ultraviolet (UV) irradiation and natural sunlight was evaluated. Among the various morphologies, ZTO nanoparticles revealed the best performance, with degradation > 90% being achieved in 60 min under UV irradiation and in 90 min under natural sunlight. The eco-friendly production process and the demonstrated ability of these nanostructures to be used in various water decontamination processes reinforces their sustainability and the role they can play in a circular economy.
In Situ Decoration of ZnSnO3 Nanosheets on the Surface of Hollow Zn2SnO4 Octahedrons for Enhanced Solar Energy Application
Authors: Li Zhengdao , Liu Kecheng , Sun Ruixue , Yang Chuanyun , Liu Xiaodi
Published: June 2022

Hierarchical ZnSnO3/Zn2SnO4 porous hollow octahedrons were constructed using the method of combining the acid etching process with the in situ decoration technique for photovoltaic and photocatalytic applications. The composite was used as photoanode of the dye-sensitized solar cells (DSSCs), an overall 4.31% photovoltaic conversion efficiency was obtained, nearly a 73.1% improvement over the DSSCs that used Zn2SnO4 solid octahedrons. The composite was also determined to be a high-performance photocatalyst for the removal of heavy metal ion Cr (VI) and antibiotic ciprofloxacin (CIP) in single and co-existing systems under simulated sunlight irradiation. It was remarkable that the composite displayed good reusability and stability in a co-existing system, and the simultaneous removal performance could be restored by a simple acid treatment. These improvements of solar energy utilization were ascribed to the synergetic effect of the hierarchical porous hollow morphology, the introduction of ZnSnO3 nanosheets, and the heterojunction formed between ZnSnO3 and Zn2SnO4, which could improve light harvesting capacity, expedite electron transport and charge-separation efficiencies.
MEMS Resonant Devices as a Revolutional Technology
Authors: Yuan Quan , Zhao Xiaoguang , Feng Yuyi
Published: June 2022

Resonance, a natural phenomenon, is a fundamental physical property of any object [...]
LncRNA-Based Classification of Triple Negative Breast Cancer Revealed Inherent Tumor Heterogeneity and Vulnerabilities
Authors: Vishnubalaji Radhakrishnan , Elango Ramesh , Alajez Nehad M.
Published: June 2022

Triple negative breast cancer (TNBC) represents a diverse group of cancers based on their gene expression profiles. While the current mRNA-based classification of TNBC has contributed to our understanding of the heterogeneity of this disease, whether such heterogeneity can be resolved employing a long noncoding RNA (lncRNA) transcriptome has not been established thus far. Herein, we used iterative clustering and guide-gene selection (ICGS) and uniform manifold approximation and projection (UMAP) dimensionality reduction analysis on a large cohort of TNBC transcriptomic data (TNBC = 360, normal = 88) and classified TNBC into four main clusters: LINC00511-enriched, LINC00393-enriched, FIRRE-enriched, and normal tissue-like. Delving into associated gene expression profiles revealed remarkable differences in canonical, casual, upstream, and functional categories among different lncRNA-derived TNBC clusters, suggesting functional consequences for altered lncRNA expression. Correlation and survival analysis comparing mRNA- and lncRNA-based clustering revealed similarities and differences between the two classification approaches. To provide insight into the potential role of the identified lncRNAs in TNBC biology, CRISPR-Cas9 mediated LINC00511 promoter deletion reduced colony formation and enhanced the sensitivity of TNBC cells to paclitaxel, suggesting a role for LINC00511 in conferring tumorigenicity and resistance to therapy. Our data revealed a novel lncRNA-based classification of TNBC and suggested their potential utilization as disease biomarkers and therapeutic targets.
Cuffless and Touchless Measurement of Blood Pressure from Ballistocardiogram Based on a Body Weight Scale
Authors: Liu Shing-Hong , Zhang Bing-Hao , Chen Wenxi , Su Chun-Hung , Chin Chiun-Li
Published: June 2022

Currently, in terms of reducing the infection risk of the COVID-19 virus spreading all over the world, the development of touchless blood pressure (BP) measurement has potential benefits. The pulse transit time (PTT) has a high relation with BP, which can be measured by electrocardiogram (ECG) and photoplethysmogram (PPG). The ballistocardiogram (BCG) reflects the mechanical vibration (or displacement) caused by the heart contraction/relaxation (or heart beating), which can be measured from multiple degrees of the body. The goal of this study is to develop a cuffless and touchless BP-measurement method based on a commercial weight scale combined with a PPG sensor when measuring body weight. The proposed method was that the PTTBCG-PPGT was extracted from the BCG signal measured by a weight scale, and the PPG signal was measured from the PPG probe placed at the toe. Four PTT models were used to estimate BP. The reference method was the PTTECG-PPGF extracted from the ECG signal and PPG signal measured from the PPG probe placed at the finger. The standard BP was measured by an electronic blood pressure monitor. Twenty subjects were recruited in this study. By the proposed method, the root-mean-square error (ERMS) of estimated systolic blood pressure (SBP) and diastolic blood pressure (DBP) are 6.7 ± 1.60 mmHg and 4.8 ± 1.47 mmHg, respectively. The correlation coefficients, r2, of the proposed model for the SBP and DBP are 0.606 ± 0.142 and 0.284 ± 0.166, respectively. The results show that the proposed method can serve for cuffless and touchless BP measurement.
Seroprevalence of Transfusion Transmissible Infections and Associated Risk Factors in Hospitalized Patients before Transfusion in Jinling Hospital Nanjing University: A Three-Year Retrospective Study
Authors: Wang Wei , Kong Xiaojun , Zhao Guangchao , Huang Xuelian , Yuan Jun , Li Na , Zhang Xiaonan , Luo Kaiyun , Luan Jianfeng , Fan Xuzhou
Published: June 2022

Transfusion-transmitted infections (TTIs), such as hepatitis B virus (HBV), hepatitis C virus (HCV), human immunodeficiency virus (HIV), and treponema pallidum (TP), must be detected before blood transfusion. However, few studies have been conducted on the prevalence and accuracy of positive results in hospitalized patients. The purpose of this study was to investigate the real seroprevalence of TTIs among patients before blood transfusion and analyze the characteristics of false-positive results in Jinling Hospital, Nanjing University, China. TTI results were collected from medical records and analyzed retrospectively. Additionally, we also used confirmatory assays to verify the accuracy of positive results. The overall prevalence of TTI was 8.96%, which was related to gender and age. The real positive rates were 86.67% (HBV), 35.09% (HCV), 20.75% (HIV), and 100% (TP). Our results also showed that high-speed centrifugation can reduce the false-positive rate of HBsAg. In summary, the results demonstrated that the positive rates of TTIs in hospitalized patients are higher than those in the general population. We also confirmed the existence of false-positive results in serological screening for TTIs. The method of processing specimens through high-speed centrifugation could reduce the false-positive results of detecting antigens effectively.
Attitudes and Knowledge of, and Preferences for Learning about Cultural Competence: A Study of Pharmacy Students from One Australian Pharmacy Program
Authors: Nkhoma Gloria , Lim Chiao Xin , Kennedy Gerard A. , Stupans Ieva
Published: June 2022

Culturally and linguistically diverse populations, particularly asylum seekers, face challenges in accessing healthcare services. Pharmacists need to be capable of identifying and responding appropriately to the needs of diverse population groups. The aims of this study were to clarify student pharmacists’: knowledge of, and attitudes to, asylum seekers; their understanding of themselves with regard to cultural competence; their exposure to culturally and linguistically diverse clinical settings; their potential receptivity to learning opportunities directed towards cultural competence; and the extent to which they interpreted the current curriculum as improving their cultural competence. Pharmacy students’ viewpoints and perspectives were essential as emerging pharmacy professionals. This study employed mixed methods and convenience sampling. There were no significant (p > 0.05) associations between demographics and any of the survey items. Five themes emerged from the interviews: namely, exposure, formal vs. informal, positive views, conflict, and sufficiency. Pharmacy curriculum should ideally provide sufficient knowledge to meet culturally diverse healthcare consumers’ needs, especially asylum seekers. The most efficacious models for teaching cultural competence are as yet still undetermined. Interactive learning in cultural competence was recommended as essential.
Turing’s Conceptual Engineering
Authors: Miłkowski Marcin
Published: June 2022

Alan Turing’s influence on subsequent research in artificial intelligence is undeniable. His proposed test for intelligence remains influential. In this paper, I propose to analyze his conception of intelligence by relying on traditional close reading and language technology. The Turing test is interpreted as an instance of conceptual engineering that rejects the role of the previous linguistic usage, but appeals to intuition pumps instead. Even though many conceive his proposal as a prime case of operationalism, it is more plausibly viewed as a stepping stone toward a future theoretical construal of intelligence in mechanical terms. To complete this picture, his own conceptual network is analyzed through the lens of distributional semantics over the corpus of his written work. As it turns out, Turing’s conceptual engineering of the notion of intelligence is indeed quite similar to providing a precising definition with the aim of revising the usage of the concept. However, that is not its ultimate aim: Turing is after a rich theoretical understanding of thinking in mechanical, i.e., computational, terms.
Serial-Omics and Molecular Function Study Provide Novel Insight into Cucumber Variety Improvement
Authors: Han Danni , Ma Xiaojun , Zhang Lei , Zhang Shizhong , Sun Qinghua , Li Pan , Shu Jing , Zhao Yanting
Published: June 2022

Cucumbers are rich in vitamins and minerals. The cucumber has recently become one of China’s main vegetable crops. More specifically, the adjustment of the Chinese agricultural industry’s structure and rapid economic development have resulted in increases in the planting area allocated to Chinese cucumber varieties and in the number of Chinese cucumber varieties. After complete sequencing of the “Chinese long” genome, the transcriptome, proteome, and metabolome were obtained. Cucumber has a small genome and short growing cycle, and these traits are conducive to the application of molecular breeding techniques for improving fruit quality. Here, we review the developments and applications of molecular markers and genetic maps for cucumber breeding and introduce the functions of gene families from the perspective of genomics, including fruit development and quality, hormone response, resistance to abiotic stress, epitomizing the development of other omics, and relationships among functions.
Effects of Magnesium, Pyrophosphate and Phosphonates on Pyrophosphorolytic Reaction of UDP-Glucose Pyrophosphorylase
Authors: Kleczkowski Leszek A. , Decker Daniel
Published: June 2022

UDP-glucose pyrophosphorylase (UGPase) carries a freely reversible reaction, using glucose-1-P and UTP to produce UDP-glucose (UDPG) and pyrophosphate (PPi), with UDPG being essential for glycosylation reactions in all organisms including, e.g., synthesis of sucrose, cellulose and glycoproteins. In the present study, we found that free magnesium (Mg2+) had profound effects on the reverse reaction of purified barley UGPase, and was absolutely required for its activity, with an apparent Km of 0.13 mM. More detailed analyses with varied concentrations of MgPPi allowed us to conclude that it is the MgPPi complex which serves as true substrate for UGPase in its reverse reaction, with an apparent Km of 0.06 mM. Free PPi was an inhibitor in this reaction. Given the key role of PPi in the UGPase reaction, we have also tested possible effects of phosphonates, which are analogs of PPi and phosphate (Pi). Clodronate and etidronate (PPi analogs) had little or no effect on UGPase activity, whereas fosetyl-Al (Pi analog), a known fungicide, acted as effective near-competitive inhibitor versus PPi, with Ki of 0.15 mM. The data are discussed with respect to the role of magnesium in the UGPase reaction and elucidating the use of inhibitors in studies on cellular function of UGPase and related enzymes.
Comparison of Methoxypyrazine Content and Expression Pattern of O-Methyltransferase Genes in Grape Berries and Wines from Six Cultivars (Vitis vinifera L.) in the Eastern Foothill of the Helan Mountain
Authors: Zhang Yanxia , Li Xiangyi , Guo Xufeng , Wang Ning , Geng Kangqi , Li Dongmei , Wang Zhenping
Published: June 2022

Methoxypyrazines (MPs) are a unique class of volatile compounds containing nitrogenous heterocyclics that impart green bell, vegetal and herbal odors to red grape berries and wines. In this study, the quality and MPs levels of grape berries from six representative red wine grape varieties were determined in the two consecutive years. The results showed that, at maturity, the highest total soluble solid was observed in Petit Verdot grape berries in the two consecutive years. While the anthocyanin content showed the highest in Marselan berries in 2018, in 2019, Petit Verdot berries had the highest anthocyanin content. Moreover, 2-methoxypyrazine (MOMP), 3-methyl-2-methoxypyrazine (MEMP) and 3-ethyl-2-methoxypyrazine (ETMP) levels were relatively lower, with almost no detectable in berries at maturity. The relative higher 3-isobutyl-2-methoxypyrazine (IBMP) content was observed in Cabernet Sauvignon, Marselan, Merlot, and Malbec berries. However, 3-sec-butyl-2-methoxypyrazine (SBMP) and IBMP were only detected in six wines, and their levels were higher than those in the grape berries. Furthermore, correlation analysis showed that there was a statistically positive correlation between the expression levels of VvOMT1 and VvOMT3 and MPs content in grape berries, while the lowest association was found in the VvOMT2. These findings provide a basis for selecting the most suitable grape varieties to improve wine quality.
Does Abiotic Host Stress Favour Dothideomycete-Induced Disease Development?
Authors: Röhrig Laura , Dussart Francois
Published: June 2022

Dothideomycetes represent one of the largest and diverse class of fungi. This class exhibits a wide diversity of lifestyles, including endophytic, saprophytic, pathogenic and parasitic organisms. Plant pathogenic fungi are particularly common within the Dothideomycetes and are primarily found within the orders of Pleosporales, Botryosphaeriales and Capnodiales. As many Dothideomycetes can infect crops used as staple foods around the world, such as rice, wheat, maize or banana, this class of fungi is highly relevant to food security. In the context of climate change, food security faces unprecedented pressure. The benefits of a more plant-based diet to both health and climate have long been established, therefore the demand for crop production is expected to increase. Further adding pressure on food security, both the prevalence of diseases caused by fungi and the yield losses associated with abiotic stresses on crops are forecast to increase in all climate change scenarios. Furthermore, abiotic stresses can greatly influence the outcome of the host-pathogen interaction. This review focuses on the impact of abiotic stresses on the host in the development of diseases caused by Dothideomycete fungi.
Advances in Genetic Analysis and Breeding of Cassava (Manihot esculenta Crantz): A Review
Authors: Amelework Assefa B. , Bairu Michael W.
Published: June 2022

Cassava (Manihot esculenta Crantz) is the sixth most important food crop and consumed by 800 million people worldwide. In Africa, cassava is the second most important food crop after maize and Africa is the worlds’ largest producer. Though cassava is not one of the main commodity crops in South Africa, it is becoming a popular crop among farming communities in frost-free areas, due to its climate-resilient nature. This necessitated the establishment of a multi-disciplinary research program at the Agricultural Research Council of South Africa. The objective of this review is to highlight progress made in cassava breeding and genetic analysis. This review highlights the progress of cassava research worldwide and discusses research findings on yield, quality, and adaptability traits in cassava. It also discusses the limitations and the prospects of the cassava R&D program towards development of the cassava industry in South Africa.
Assessment of Artificial Intelligence Strategies to Estimate the Strength of Geopolymer Composites and Influence of Input Parameters
Authors: Khan Kaffayatullah , Ahmad Waqas , Amin Muhammad Nasir , Ahmad Ayaz , Nazar Sohaib , Al-Faiad Majdi Adel
Published: June 2022

Geopolymers might be the superlative alternative to conventional cement because it is produced from aluminosilicate-rich waste sources to eliminate the issues associated with its manufacture and use. Geopolymer composites (GPCs) are gaining popularity, and their research is expanding. However, casting, curing, and testing specimens requires significant effort, price, and time. For research to be efficient, it is essential to apply novel approaches to the said objective. In this study, compressive strength (CS) of GPCs was anticipated using machine learning (ML) approaches, i.e., one single method (support vector machine (SVM)) and two ensembled algorithms (gradient boosting (GB) and extreme gradient boosting (XGB)). All models’ validity and comparability were tested using the coefficient of determination (R2), statistical tests, and k-fold analysis. In addition, a model-independent post hoc approach known as SHapley Additive exPlanations (SHAP) was employed to investigate the impact of input factors on the CS of GPCs. In predicting the CS of GPCs, it was observed that ensembled ML strategies performed better than the single ML technique. The R2 for the SVM, GB, and XGB models were 0.98, 0.97, and 0.93, respectively. The lowered error values of the models, including mean absolute and root mean square errors, further verified the enhanced precision of the ensembled ML approaches. The SHAP analysis revealed a stronger positive correlation between GGBS and GPC′s CS. The effects of NaOH molarity, NaOH, and Na2SiO3 were also observed as more positive. Fly ash and gravel size: 10/20 mm have both beneficial and negative impacts on the GPC′s CS. Raising the concentration of these ingredients enhances the CS, whereas increasing the concentration of GPC reduces it. Gravel size: 4/10 mm has less favorable and more negative effects. ML techniques will benefit the construction sector by offering rapid and cost-efficient solutions for assessing material characteristics.
A Study on Preparation and Property Evaluations of Composites Consisting of TPU/Triclosan Membranes and Tencel®/LMPET Nonwoven Fabrics
Authors: Shiu Bing-Chiuan , Hsu Po-Wen , Lin Jian-Hong , Chien Ling-Fang , Lin Jia-Horng , Lou Ching-Wen
Published: June 2022

This study investigated eco-friendly antibacterial medical protective clothing via the nonwoven process and characteristic evaluations. Firstly, Tencel® fibers and low melting point polyester (LMPET) fibers (re-sliced and granulated from recycled PET bottles) were mixed at different ratios and then needle punched at diverse needle rolling depths. The influences of manufacturing parameters on the Tencel®/LMPET nonwoven fabrics were examined in terms of mechanical properties, water vapor transmission rate, and stiffness. Next, Tencel®/LMPET nonwoven fabrics were combined with thermoplastic polyurethane (TPU)/Triclosan antibacterial membranes that contained different contents of triclosan using melt processing technology. The resulting Tencel®/LMPET/TPU/Triclosan composites were characterized via different measurements; an optimal bursting strength of 86.86 N, an optimal horizontal tensile strength of 41.90 N, and an optimal stiffness along the MD and CD of 8.60 cm were recorded. Furthermore, the Tencel®/LMPET/TPU/Triclosan composites exhibited a distinct inhibition zone in the antibacterial measurement, and the hydrostatic pressure met the requirements of the EN 14126:2003 and GB 19082-200 disposable medical protective gear test standards.
From Foods to Chemotherapeutics: The Antioxidant Potential of Dietary Phytochemicals
Authors: Briggs Michelle A.
Published: June 2022

Food plants have been recognized for their medicinal properties for millennia, a concept supported by epidemiological studies indicating long-term health benefits for people consuming greater amounts of fruits and vegetables. As our technology and instrumentation advance, researchers have the ability to identify promising phytochemicals, and examine their potential benefits, or detriments, to human health. While results from trials investigating single chemical supplementation have sometimes produced negative health results, studies investigating the synergistic action of phytochemicals—either within our diet or as an adjuvant to radiation or chemotherapy—appear promising. Utilizing phytochemicals as synergistic agents may lower the chemotherapeutic doses needed to incur physiological results, while also using chemicals with fewer toxic effects. This review investigates a variety of plant-produced chemicals humans typically ingest, their impacts on overall health patterns, molecular mechanisms associated with their health impacts, and the potential of their synergistic use for therapeutic purposes.
An Early Medieval Śaiva Pilgrimage Landscape: The Persistence of Pampa and Bhairava in the Hemakuta Hill Sacred Space, 800–1325 CE
Authors: Haak Candis
Published: June 2022

The early medieval Pampa tirtha (pilgrimage), in the Hampi area, Bellary District, Karnataka, South India, is largely presented in research as a relatively homogenous, albeit sacred space. This paper describes a nuanced understanding of the Pampa tirtha through the lens of spatial organization and pilgrim movement. The natural and built landscape features of the area were digitized through Esri’s ArcMap to historically situate extant stone monuments. Devotee movement through the pilgrimage space was then modelled on time-sensitive maps of architectural and natural features. Pathways of movement across the site were subsequently explored in the immersive panoramic imagery captured in Google Street View. By combining these digital tools, a historicized analysis of the character and qualities of place, born from the organization of the site, are identifiable. The results demonstrate how devotees moved through a network of distinct nodes of shrines, temples, and gateways. Each node possessed a unique relationship to microtopographic features of the hill, and to the earliest deities of the site that originally anchored and oriented the sacred space: Pampa and Bhairava. The pilgrimage space that developed between these two deities was tied together through a path of movement, running south to north. Trends of re-ordering the Pampa tirtha spatial network also reveal patron and artisan mechanisms to privilege and prioritize the 12th-century addition of the god Virupaksha into the sacred space.
Atmospheric Effects and Precursors of Rainfall over the Swiss Plateau
Authors: Wang Wenyue , Hocke Klemens
Published: June 2022

In this study, we investigate the characteristics of atmospheric parameters before, during, and after rain events in Bern, Switzerland. Ground-based microwave radiometer data of the TROpospheric WAter RAdiometer (TROWARA) with a time resolution of 7 s, observations of a weather station, and the composite analysis method are used to derive the temporal evolution of rain events and to identify possible rainfall precursors during a 10-year period (1199 available rain events). A rainfall climatology is developed using parameters integrated water vapor (IWV), integrated liquid water (ILW), rain rate, infrared brightness temperature (TIR), temperature, pressure, relative humidity, wind speed, and air density. It was found that the IWV is reduced by about 2.2 mm at the end of rain compared to the beginning. IWV and TIR rapidly increase to a peak at the onset of the rainfall. Precursors of rainfall are that the temperature reaches its maximum around 30 to 60 min before rain, while the pressure and relative humidity are minimal. IWV fluctuates the most before rain (obtained with a 10 min bandpass). In 60% of rain events, the air density decreases 2 to 6 h before the onset of rain. The seasonality and the duration of rain events as well as the diurnal cycle of atmospheric parameters are also considered. Thus, a prediction of rainfall is possible with a true detection rate of 60% by using the air density as a precursor. Further improvements in the nowcasting of rainfall are possible by using a combination of various atmospheric parameters which are monitored by a weather station and a ground-based microwave radiometer.
Positioning of Quadruped Robot Based on Tightly Coupled LiDAR Vision Inertial Odometer
Authors: Gao Fangzheng , Tang Wenjun , Huang Jiacai , Chen Haiyang
Published: June 2022

Quadruped robots, an important class of unmanned aerial vehicles, have broad potential for applications in education, service, industry, military, and other fields. Their independent positioning plays a key role for completing assigned tasks in a complex environment. However, positioning based on global navigation satellite systems (GNSS) may result in GNSS jamming and quadruped robots not operating properly in environments sheltered by buildings. In this paper, a tightly coupled LiDAR vision inertial odometer (LVIO) is proposed to address the positioning inaccuracy of quadruped robots, which have poor mileage information obtained though legs and feet structures only. With this optimization method, the point cloud data obtained by 3D LiDAR, the image feature information obtained by binocular vision, and the IMU inertial data are combined to improve the precise indoor and outdoor positioning of a quadruped robot. This method reduces the errors caused by the uniform motion model in laser odometer as well as the image blur caused by rapid movements of the robot, which can lead to error-matching in a dynamic scene; at the same time, it alleviates the impact of drift on inertial measurements. Finally, the quadruped robot in the laboratory is used to build a physical platform for verification. The experimental results show that the designed LVIO effectively realizes the positioning of four groups of robots with high precision and strong robustness, both indoors or outdoors, which verify the feasibility and effectiveness of the proposed method.
Damage Properties of the Block-Stone Embankment in the Qinghai–Tibet Highway Using Ground-Penetrating Radar Imagery
Authors: Qi Shunshun , Li Guoyu , Chen Dun , Chai Mingtang , Zhou Yu , Du Qingsong , Cao Yapeng , Tang Liyun , Jia Hailiang
Published: June 2022

The block-stone embankment is a special type of embankment widely used to protect the stability of the underlying warm and ice-rich permafrost. Under the influence of multiple factors, certain damages will still occur in the block-stone embankment after a period of operation, which may weaken or destroy its cooling function, introducing more serious damages to the Qinghai–Tibet Highway (QTH). Ground-penetrating radar (GPR), a nondestructive testing technique, was adopted to investigate the damage properties of the damaged block-stone embankment. GPR imagery, together with the other data and methods (structural characteristics, field survey data, GPR parameters, etc.), indicated four categories of damage: (i) loosening of the upper sand-gravel layer; (ii) loosening of the block-stone layer; (iii) settlement of the block-stone layer; and (iv) dense filling of the block-stones layer. The first two conditions were widely distributed, whereas the settlement and dense filling of the block-stone layer were less so, and the other combined damages also occurred frequently. The close correlation between the different damages indicated a causal relationship. A preliminary discussion of these observations about the influences on the formation of the damage of the block-stone embankment is included. The findings provide some points of reference for the future construction and maintenance of block-stone embankments in permafrost regions.
Biosensors for Klebsiella pneumoniae with Molecularly Imprinted Polymer (MIP) Technique
Authors: Pintavirooj Chuchart , Vongmanee Naphatsawan , Sukjee Wannisa , Sangma Chak , Visitsattapongse Sarinporn
Published: June 2022

Nosocomial infection is one of the most important problems that occurs in hospitals, as it directly affects susceptible patients or patients with immune deficiency. Klebsiella pneumoniae (K. pneumoniae) is the most common cause of nosocomial infections in hospitals. K. pneumoniae can cause various diseases such as pneumonia, urinary tract infections, septicemias, and soft tissue infections, and it has also become highly resistant to antibiotics. The principal routes for the transmission of K. pneumoniae are via the gastrointestinal tract and the hands of hospital personnel via healthcare workers, patients, hospital equipment, and interventional procedures. These bacteria can spread rapidly in the hospital environment and tend to cause nosocomial outbreaks. In this research, we developed a MIP-based electrochemical biosensor to detect K. pneumoniae. Quantitative detection was performed using an electrochemical technique to measure the changes in electrical signals in different concentrations of K. pneumoniae ranging from 10 to 105 CFU/mL. Our MIP-based K. pneumoniae sensor was found to achieve a high linear response, with an R2 value of 0.9919. A sensitivity test was also performed on bacteria with a similar structure to that of K. pneumoniae. The sensitivity results show that the MIP-based K. pneumoniae biosensor with a gold electrode was the most sensitive, with a 7.51 (% relative current/log concentration) when compared with the MIP sensor applied with Pseudomonas aeruginosa and Enterococcus faecalis, where the sensitivity was 2.634 and 2.226, respectively. Our sensor was also able to achieve a limit of detection (LOD) of 0.012 CFU/mL and limit of quantitation (LOQ) of 1.61 CFU/mL.