bluuebunny/crossref_metadata_2025 huggingface dataset:
Insights into the Virulence of Campylobacter jejuni Associated with Two-Component Signal Transduction Systems and Single Regulators
Authors: Gahamanyi Noel , Song Dae-Geun , Mboera Leonard E. G. , Matee Mecky I. , Mutangana Dieudonné , Amachawadi Raghavendra G. , Komba Erick V. G. , Pan Cheol-Ho
Published: May 2022

Campylobacter jejuni is one of the major aetiologies of diarrhoea. Understanding the processes and virulence factors contributing to C. jejuni fitness is a cornerstone for developing mitigation strategies. Two-component signal transduction systems, known as two-component systems (TCSs), along with single regulators with no obvious cognate histidine kinase, help pathogens in interacting with their environments, but the available literature on C. jejuni is limited. A typical TCS possesses histidine kinase and response regulator proteins. The objective of this review was to provide insights into the virulence of C. jejuni associated with TCSs and single regulators. Despite limited research, TCSs are important contributors to the pathogenicity of C. jejuni by influencing motility (FlgSR), colonisation (DccRS), nutrient acquisition (PhosSR and BumSR), and stress response (RacRS). Of the single regulators, CbrR and CosR are involved in bile resistance and oxidative stress response, respectively. Cross-talks among TCSs complicate the full elucidation of their molecular mechanisms. Although progress has been made in characterising C. jejuni TCSs, shortfalls such as triggering signals, inability to induce mutations in some genes, or developing suitable in vivo models are still being encountered. Further research is expected to shed light on the unexplored sides of the C. jejuni TCSs, which may allow new drug discoveries and better control strategies.
Evaluation of S1RBD-Specific IgG Antibody Responses following COVID-19 Vaccination in Healthcare Professionals in Cyprus: A Comparative Look between the Vaccines of Pfizer-BioNTech and AstraZeneca
Authors: Deeba Elie , Krashias George , Constantinou Astero , Koptides Dana , Lambrianides Anastasia , Christodoulou Christina
Published: May 2022

There is an ongoing effort to report data on SARS-CoV-2 antibodies in different individuals. Ninety-seven healthcare workers were enrolled in this study (Pfizer’s BNT162b2, n = 52; and AstraZeneca’s ChAdOx1-S, n = 45) and S1RBD-specific IgG antibodies were analyzed over time. Both vaccines induced S1RBD-specific antibodies after the second dose. A significant increase in S1RBD-specific IgG median levels 3 weeks following the second dose was detected (BNT162b2, 118.0 BAU/mL to 2018.0 BAU/mL; ChAdOx1-S, 38.1 BAU/mL to 182.1 BAU/mL). At 3 months post the second dose, a significant decrease in S1RBD-specific IgG median levels was also evident (BNT162b2, 415.6 BAU/mL, ChAdOx1-S, 84.7 BAU/mL). The elimination rate of these antibodies was faster in BNT162b2- rather than ChAdOx1-S- vaccinated individuals. A booster dose induced a significant increase in the S1RBD-specific IgG median levels (BNT162b2, 1823.0 BAU/mL; ChAdOx1-S, 656.8 BAU/mL). This study is the first of its kind to characterize S1RBD-specific IgG antibody responses in vaccinated healthcare workers in Cyprus. While the positivity for S1RBD-specific antibodies was maintained 3 months after the second vaccine dose, the level of these antibodies waned over the same period, indicating the importance of a booster vaccination. The results herein could complement the public health policies regarding the immunization schedule for COVID-19.
Biopriming of Durum Wheat Seeds with Endophytic Diazotrophic Bacteria Enhances Tolerance to Fusarium Head Blight and Salinity
Authors: Hadj Brahim Adel , Ben Ali Manel , Daoud Lobna , Jlidi Mouna , Akremi Ismahen , Hmani Houda , Feto Naser Aliye , Ben Ali Mamdouh
Published: May 2022

There is growing interest in the use of bio inoculants based on plant growth-promoting bacteria (PGPB) to promote plant growth under biotic and abiotic stresses. Here, we provided a detailed account of the effectiveness of a number of endophytic PGPB strains, isolated from the roots of the halophyte Salicornia brachiata in promoting durum wheat growth and enhancing its tolerance to salinity and fusarium head blight (FHB) disease. Bacillus spp. strains MA9, MA14, MA17, and MA19 were found to have PGPB characteristics as they produced indole-3-acetic acid, siderophores, and lytic enzymes, fixed free atmospheric nitrogen, and solubilized inorganic phosphate in vitro. Additionally, the in vivo study that involved in planta inoculation assays under control and stress conditions indicated that all PGPB strains significantly (p < 0.05) increased the total plant length, dry weight, root area, seed weight, and nitrogen, protein, and mineral contents. Particularly, the MA17 strain showed a superior performance since it was the most efficient in reducing disease incidence in wheat explants by 64.5%, in addition to having the strongest plant growth promotion activity under salt stress. Both in vitro and in vivo assays showed that MA9, MA14, MA17, and MA19 strains were able to play significant PGPB roles. However, biopriming with Bacillus subtilis MA17 offered the highest plant growth promotion and salinity tolerance, and bioprotection against FHB. Hence, it would be worth testing the MA17 strain under field conditions as a step towards its commercial production. Moreover, the strain could be further assessed for its plausible role in bioprotection and growth promotion in other crop plants. Thus, it was believed that the strain has the potential to significantly contribute to wheat production in arid and semi-arid regions, especially the salt-affected Middle Eastern Region, in addition to its potential role in improving wheat production under biotic and abiotic stresses in other parts of the world.
Dynamic Propagation and Electro-Mechanical Characteristics of New Microcracks in Notched Coal Samples Studied by the Three-Point Bending Test System and AFM
Authors: Wang Weixiang , Song Dazhao , He Xueqiu , Liu Qingxia , Li Zhenlei , Qiu Liming , Mei Guodong
Published: May 2022

This work extends research on the mechanism of electromagnetic radiation (EMR) induced by coal or rock fractures to the category of microscopic dynamic experimental research. A custom-made three-point bending test system and atomic force microscope (AFM) were integrated to obtain the microdynamic loading test system. The notched coal samples were prepared specially. The dynamic propagation of new microcracks in coal samples was measured, and the propagation velocity was calculated. The morphology and electro-mechanical characteristics of new microcracks were tested. More importantly, the causes of the changes in the electro-mechanical characteristics before and after fracture were analyzed, and the effects of these changes on the EMR were discussed. The results showed that the average propagation velocities during the same time interval are 9.5 μm/s, 12.1 μm/s, and 16.2 μm/s. The elastic modulus of the material at the microcrack edge is generally smaller than that of the material in other locations, while the adhesion and deformation are larger. Moreover, the closer the material is to the microcrack, the higher its surface potential. The electrons generated at the microcrack edge and emitted into the atmosphere, which made the greater potentials of the microcrack edge. Many electrons with different velocities and directions migrate in similar parallel-plate capacitors, which are formed by the relative microscale surface of the coal microcrack tip and have different field strengths, resulting in EMR with complex frequencies and different intensities. This study provides a micro-dynamic experimental basis for research on the electromagnetic radiation mechanism.
Inhibitory Effects and Mechanism of the Natural Compound Diaporthein B Extracted from Marine-Derived Fungi on Colon Cancer Cells
Authors: Tang Peihua , Liu Dandan , Wu Zheli , Cui Hui , Zhang Ren , Kuang Zaoyuan
Published: May 2022

This study aimed to investigate the inhibitory effects and mechanism of diaporthein B (DTB), a natural compound extracted from the fungus Penicillium sclerotiorum GZU-XW03-2, on human colon cancer cells. The inhibitory effect of DTB at different concentrations on the proliferation of colon cancer cells HCT116 and LOVO was detected at 24 and 48 h. The effect of cell migration and clone formation ability were detected by cell scratch and plate cloning experiments. Morphological changes were observed by Hoechst 33342 and Annexin-V/PI staining, and flow cytometry was used to detect the proportion of apoptotic cells. DTB significantly inhibited colon cancer cell proliferation, migration, and apoptosis in a dose-dependent manner without significant effects on normal colonic epithelial cells NCM460. The IC50 inhibition effect can be achieved after treatment with 3 μmol/L DTB for 24 h. Compared with the blank group, the migration and clonal-forming ability of colon cancer cells in the DTB group was significantly decreased (p < 0.01), while the apoptotic cells were significantly increased (p < 0.01) in a concentration-dependent manner. DTB can inhibit the proliferation and migration of human colon cancer cells HCT116 and LOVO and promote the apoptosis of human colon cancer cells.
Short-Term Very High Carbohydrate Diet and Gut-Training Have Minor Effects on Gastrointestinal Status and Performance in Highly Trained Endurance Athletes
Authors: King Andy J. , Etxebarria Naroa , Ross Megan L. , Garvican-Lewis Laura , Heikura Ida A. , McKay Alannah K. A. , Tee Nicolin , Forbes Sara F. , Beard Nicole A. , Saunders Philo U. , Sharma Avish P. , Gaskell Stephanie K. , Costa Ricardo J. S. , Burke Louise M.
Published: May 2022

We implemented a multi-pronged strategy (MAX) involving chronic (2 weeks high carbohydrate [CHO] diet + gut-training) and acute (CHO loading + 90 g·h−1 CHO during exercise) strategies to promote endogenous and exogenous CHO availability, compared with strategies reflecting lower ranges of current guidelines (CON) in two groups of athletes. Nineteen elite male race walkers (MAX: 9; CON:10) undertook a 26 km race-walking session before and after the respective interventions to investigate gastrointestinal function (absorption capacity), integrity (epithelial injury), and symptoms (GIS). We observed considerable individual variability in responses, resulting in a statistically significant (p < 0.001) yet likely clinically insignificant increase (Δ 736 pg·mL−1) in I-FABP after exercise across all trials, with no significant differences in breath H2 across exercise (p = 0.970). MAX was associated with increased GIS in the second half of the exercise, especially in upper GIS (p < 0.01). Eighteen highly trained male and female distance runners (MAX: 10; CON: 8) then completed a 35 km run (28 km steady-state + 7 km time-trial) supported by either a slightly modified MAX or CON strategy. Inter-individual variability was observed, without major differences in epithelial cell intestinal fatty acid binding protein (I-FABP) or GIS, due to exercise, trial, or group, despite the 3-fold increase in exercise CHO intake in MAX post-intervention. The tight-junction (claudin-3) response decreased in both groups from pre- to post-intervention. Groups achieved a similar performance improvement from pre- to post-intervention (CON = 39 s [95 CI 15–63 s]; MAX = 36 s [13–59 s]; p = 0.002). Although this suggests that further increases in CHO availability above current guidelines do not confer additional advantages, limitations in our study execution (e.g., confounding loss of BM in several individuals despite a live-in training camp environment and significant increases in aerobic capacity due to intensified training) may have masked small differences. Therefore, athletes should meet the minimum CHO guidelines for training and competition goals, noting that, with practice, increased CHO intake can be tolerated, and may contribute to performance outcomes.
Porosity Tunable Poly(Lactic Acid)-Based Composite Gel Polymer Electrolyte with High Electrolyte Uptake for Quasi-Solid-State Supercapacitors
Authors: Yang Chao , Bai Yuge , Xu Huan , Li Manni , Cong Zhi , Li Hongjie , Chen Weimeng , Zhao Bin , Han Xiaogang
Published: May 2022

The growing popularity of quasi-solid-state supercapacitors inevitably leads to the unrestricted consumption of commonly used petroleum-derived polymer electrolytes, causing excessive carbon emissions and resulting in global warming. Also, the porosity and liquid electrolyte uptake of existing polymer membranes are insufficient for well-performed supercapacitors under high current and long cycles. To address these issues, poly(lactic acid) (PLA), a widely applied polymers in biodegradable plastics is employed to fabricate a renewable biocomposite membrane with tunable pores with the help of non-solvent phase inversion method, and a small amount of poly (vinylidene fluoride-co-hexafluoropropylene) (PVDF-HFP) is introduced as a modifier to interconnect with PLA skeleton for stabilizing the porous structure and optimizing the aperture of the membrane. Owing to easy film-forming and tunable non-solvent ratio, the porous membrane possesses high porosity (ca. 71%), liquid electrolyte uptake (366%), and preferable flexibility endowing the GPE with satisfactory electrochemical stability in coin and flexible supercapacitors after long cycles. This work effectively relieves the environmental stress resulted from undegradable polymers and reveals the promising potential and prospects of the environmentally friendly membrane in the application of wearable devices.
Dietary Supplementation with Pomegranate and Onion Aqueous and Cyclodextrin Encapsulated Extracts Affects Broiler Performance Parameters, Welfare and Meat Characteristics
Authors: Vasilopoulos Stelios , Dokou Stella , Papadopoulos Georgios A. , Savvidou Soumela , Christaki Stamatia , Kyriakoudi Anastasia , Dotas Vassilios , Tsiouris Vasilios , Bonos Eleftherios , Skoufos Ioannis , Mourtzinos Ioannis , Giannenas Ilias
Published: May 2022

The purpose of this trial was to evaluate the effects of Punica granatum L. and Allium cepa L. peels aqueous and cyclodextrin extracts on broiler chicks’ performance and welfare status, as well as on the meat chemical composition and oxidative stability. A total of 120 one-day-old male Ross-308 chicks were randomly allocated to three treatments with four replicate pens (10 chicks per pen). Broiler chicks in the control group were fed typical commercial rations in mash form, based on maize and soybean meal. The rations of the other two treatments were further supplemented with the mixture of Punica granatum and Allium cepa aqueous and cyclodextrin extracts at the level of 0.1% of the feed, respectively. At the end of the trial (day 35), tissue samples were collected for analysis. Body weight (BW), feed intake (FI), average daily gain (ADG) and the feed conversion ratio (FCR) during the period of 1–10 days, 11–24 days, 25–35 days and 1–35 days were evaluated. Litter score, dry matter in litter, pododermatitis and feather score were also assessed at the end of the trial. Data were analyzed with ANOVA using SPSS v25 software. The results showed that BW, FI and FCR values did not differ among the groups. Scoring of pododermatitis, diarrhea, feather, fecal moisture, wooden breast and white stripping did not differ (p ≥ 0.05) among the groups. Punica granatum and Allium cepa aqueous and cyclodextrin extracts favorably affected (p < 0.05) meat composition, color parameters, TBARS and protein carbonyls. Diet supplementation also increased (p < 0.05) ∑n-3 fatty acids as well as ∑n-6 fatty acids in the thigh meat. The cis-4,7,10,13,16,19-Docosahexaenoic acid fatty acids in the breast meat of broilers fed with diets supplemented with the aqueous pomegranate and onion peel extracts were found to be higher (p < 0.05), while these fatty acids in the thigh meat were found increased (p < 0.05) in the cyclodextrin group. Aqueous and cyclodextrin pomegranate and onion peel extracts may provide a promising additive to the broilers diet with functional properties, in the absence of stressful conditions.
Evolving Optimised Convolutional Neural Networks for Lung Cancer Classification
Authors: Pfeffer Maximilian Achim , Ling Sai Ho
Published: May 2022

Detecting pulmonary nodules early significantly contributes to the treatment success of lung cancer. Several deep learning models for medical image analysis have been developed to help classify pulmonary nodules. The design of convolutional neural network (CNN) architectures, however, is still heavily reliant on human domain knowledge. Manually designing CNN design solutions has been shown to limit the data’s utility by creating a co-dependency on the creator’s cognitive bias, which urges the development of smart CNN architecture design solutions. In this paper, an evolutionary algorithm is used to optimise the classification of pulmonary nodules with CNNs. The implementation of a genetic algorithm (GA) for CNN architectures design and hyperparameter optimisation is proposed, which approximates optimal solutions by implementing a range of bio-inspired mechanisms of natural selection and Darwinism. For comparison purposes, two manually designed deep learning models, FractalNet and Deep Local-Global Network, were trained. The results show an outstanding classification accuracy of the fittest GA-CNN (91.3%), which outperformed both manually designed models. The findings indicate that GAs pose advantageous solutions for diagnostic challenges, the development of which may to be fully automated in the future using GAs to design and optimise CNN architectures for various clinical applications.
Analysis of Large Vehicle Interference on Transiting Test for Measuring Building Wind Pressure Coefficient
Authors: Han Huichao , Zhang Shanzhong , Wu Yibin , Cui Yi , Liu Xin , Li Shengli
Published: May 2022

As a new method for structural wind engineering research, the transiting test has good application prospects. However, in the road environment, the experimental results will inevitably be influenced by other vehicles (especially larger vehicles). In this paper, a large vehicle was set as an interference vehicle to drive in specific conditions, and the symmetrical CAARC standard model was used to investigate the large vehicle’s interference on the transiting test method for measuring building wind pressure coefficient. The results indicate that the wind pressure coefficient time history will appear as a clear up and down undulation phenomenon during the overtaking interference period instead of the single convex phenomenon. The mean wind pressure coefficient in the negative pressure area during the overtaking interference period is lower than that in the no-interference situation. The overtaking interference features are clear and easy to distinguish, and the interference duration is long (more than 6 s). In contrast, the overtaking interference when the test vehicle is overtaken is greater. For following driving, the wake of the large vehicle no longer interferes with the transiting test results after the vehicle spacing exceeds 70 m.
Конфликты государственных интересов: от теории к аналитическим примерам хозяйственной практики
Authors:   Главное управление криминалистики Следственного комитета Российской Федерации, Опальский Александр Павлович ,   Мурманский филиал РАНХиГС, Алешин Александр Сергеевич ,   Некоммерческое партнерство "Коллегия ревизоров, экспертов и специалистов", Ушанов Пётр Владимирович ,   Некоммерческое партнерство "Коллегия ревизоров, экспертов и специалистов"
Published: May 2022

Государство – специфический хозяйствующий субъект. При всем многообразии выполняемых от имени государства задач в совершаемых действиях с государственными средствами и имуществом законный интерес государства на общегносеологическом уровне никак не определен. В статье сделан акцент на необходимости универсальных подходов для разработки критериев оценки нарушенного государственного интереса с позиции эксперта-экономиста, оценивающего эффективность принятых управленческих решений.
Современные особенности и противоречия развития экономической безопасности в России
Authors:   Пермский государственный национальный исследовательский университет, Толпышев Григорий Валерьевич , Светлаков Андрей Геннадьевич ,   Пермский государственный аграрно-технологический университет имени Д.Н. Прянишникова
Published: May 2022

В статье рассматриваются концептуальные положения экономической безопасности России. Авторами проанализированы существующие подходы к определению понятия «экономическая безопасность». Сделан вывод, что на обеспечение экономической безопасности государства напрямую влияют социальные, техногенные, информационные и другие процессы. На примере ситуации в России выявлены основные направления, воздействием на которые с помощью экономических инструментов можно нанести существенный урон безопасности в контексте проблем «экономической устойчивости» на национальном и региональном уровне. Дана оценка ключевым угрозам экономической безопасности Российской Федерации.
Queensland Fruit Fly Invasion of New Zealand: Predicting Area Suitability Under Future Climate Change Scenarios
Authors: Aguilar Glenn , Blanchon Dan , Foote Hamish , Pollonais Christina , Mosee Asia
Published: May 2022

The Queensland fruit fly (Bactrocera tryoni) is a significant horticultural pest in Australia, and has also established in other parts of the Pacific. There is a significant risk to New Zealand of invasion by this species, and several recent incursions have occurred. The potential effects of climate change on the distribution and impacts of invasive species are well known. This paper uses species distribution modelling using Maxent to predict the suitability of New Zealand to the Queensland fruit fly based on known occurrences worldwide and Bioclim climatic layers. Under current climatic conditions the majority of the country was generally in the lower range, with some areas in the medium range. Suitability prediction maps under future climate change conditions in 2050 and 2070, at lower emission (RCP 2.6) and higher emission (RCP 8.5) scenarios generally show an increase in suitability in both the North and South Islands. Calculations of the shift of suitable areas show a general movement of the centroid towards the south-east, with the higher emission scenario showing a greater magnitude of movement.
Una experiencia de Campo: la construcción de un lazo con el otro
Authors: Pranich Gabriel
Published: May 2022

El propósito en este trabajo es transmitir una experiencia como alumno del Crédito de Campo en Ciencias de la Educación dentro del Focalizado de Psicopedagogía (FFyL, UBA) durante el segundo cuatrimestre de 2018. Con el fin de intentar una aproximación a la construcción del lazo en y desde un territorio situado en una trama compleja. Consideremos que el lazo lo creamos entre las personas por medio de la interpelación. Por lo mismo nos preguntamos: ¿cómo la interpelación con el otro hace experiencia?
Campo y territorio de la Extensión como práctica académica
Authors: Zavaro Pérez Mg. Carlos A.
Published: May 2022

La extensión universitaria ha sido una práctica académica que ha ido construyéndose a partir de las experiencias cotidianas, configurando en ese devenir relaciones con el territorio que la definen y ubican en diferentes paradigmas. En este sentido, algunas de sus particularidades, entendidas como descriptores, permiten diferenciarla de otras funciones académicas como la investigación y la docencia, a la vez que posibilitan enmarcarla -en mi opinión- bajo la teoría de campos que distingue la obra de Pierre Bourdieu. De esta manera me propongo analizar algunas de las características de la extensión, enfatizando en la impronta que ha tenido el territorio y los sujetos que lo habitan en su conformación como campo, discutiendo algunos conceptos y categorías como capital cultural, trayectorias, subjetividades, tensiones, diálogo, horizontalidad, propósito y habitus que contribuyen a su demarcación, pero enfocando la discusión en el modo en que esa dinámica, que configura el campo en su estructura, permite establecer nexos y articulaciones con los otros campos académicos, posibilitando la dilución de algunos de los límites que los diferenciaban, al constituirse en protagonista de la práctica integral, e integradora, en la que estos convergen.
Low-Energy Protons in Strong-Field Dissociation of H
2
+
via Dipole-Transitions at Large Bond Lengths
Authors: Pan Shengzhe , Hu Chenxi , Zhang Zhaohan , Lu Peifen , Lu Chenxu , Zhou Lianrong , Wang Jiawei , Sun Fenghao , Qiang Junjie , Li Hui , Ni Hongcheng , Gong Xiaochun , He Feng , Wu Jian
Published: May 2022

More than ten years ago, the observation of the low-energy structure in the photoelectron energy spectrum, regarded as an “ionization surprise,” has overthrown our understanding of strong-field physics. However, the similar low-energy nuclear fragment generation from dissociating molecules upon the photon energy absorption, one of the well-observed phenomena in light-molecule interaction, still lacks an unambiguous mechanism and remains mysterious. Here, we introduce a time-energy-resolved manner using a multicycle near-infrared femtosecond laser pulse to identify the physical origin of the light-induced ultrafast dynamics of molecules. By simultaneously measuring the bond-stretching times and photon numbers involved in the dissociation of H
2
+
driven by a polarization-skewed laser pulse, we reveal that the low-energy protons (below 0.7 eV) are produced via dipole-transitions at large bond lengths. The observed low-energy protons originate from strong-field dissociation of high vibrational states rather than the low ones of H
2
+
cation, which is distinct from the well-accepted bond-softening picture. Further numerical simulation of the time-dependent Schrödinger equation unveils that the electronic states are periodically distorted by the strong laser field, and the energy gap between the field-dressed transient electronic states may favor the one- or three-photon transitions at the internuclear distance larger than 5 a.u. The time-dependent scenario and our time-energy-resolved approach presented here can be extended to other molecules to understand the complex ultrafast dynamics.
Energy and Exergetic Evaluation of Thermodynamic Systems Applied to Water Hearing at Low Temperatures
Authors: Geovana Pires Araujo Lima  , Luccas Barbosa Carneiro  , Alex Álisson Bandeira Santos  , Josiane Dantas Viana Barbosa
Published: May 2022

Given the changes in human needs in the 21st century, which are increasingly dependent on different energy sources, this work aims to compare the energetic and exergetic efficiency of a shower heated by electric resistance and a solar heater. The method employed is based on the first and second principles of thermodynamics. We found that, under conditions at low heating temperature, the shower has better exergetic efficiency due to solar heating when compared to electrical energy since the destroyed exergy is lesser in the solar heater. Energy efficiency (quantitative aspect) is bigger in electrical resistance because presents better performing energy than solar energy. Therefore, for the final use, of heating at low temperatures, the solar heater is recommended from the thermodynamic perspective.
Pain Syndromes Associated with COVID-19 and Main Approaches to Their Treatment
Authors: Аленикова О.А.
Published: May 2022

В статье представлена информация о наиболее часто встречающихся болевых синдромах, ассоциированных с COVID-19. Механизмы возникновения боли связаны с особенностями взаимодействия вируса с рецептором ангиотензинпревращающего фермента 2, вовлеченного в процессы ноцицепции, высвобождением провоспалительных медиаторов, повышающих чувствительность ноцицептивных рецепторов к медиаторам боли, а также активацией тригеминоваскулярной системы и менингеальных ноцицепторов. Наиболее рациональным является всесторонний подход к ведению пациентов с COVID-19-ассоциированными болевыми синдромами с использованием как фармакологических, так и немедикаментозных методов лечения.

The article provides information on the most common pain syndromes associated with COVID-19. The mechanisms of pain occurrence are associated with the specific effects of the virus with the angiotensin-converting enzyme 2 receptor involved in the processes of nociception, the release of pro-inflammatory mediators that increase the sensitivity of nociceptive receptors to pain mediators, as well as the activation of the trigeminovascular system and meningeal nociceptors. The most rational is a comprehensive approach to the management of patients with COVID-19-associated pain syndromes using both pharmacological and non-pharmacological methods of treatment.
In Vitro Biological Activities Of Ranunculus gracilis Clarke Rhizome
Authors: ÇİL Elif , BÖRÇEK KASURKA Ceren , ÇOL AYVAZ Melek
Published: May 2022

R. gracilis rhizomes were picked from Yenice Forests Karabük province in Turkey. Ethanol was chosen for extraction solvent. Disc diffusion method including filamentous and non-filamentous Gram-positive bacteria, Gram-negative bacteria, and yeast strains was used to evaluate the antimicrobial activity of the extract. DPPH free radical scavenging activity and ferric reducing antioxidant power analysis were performed to determine antioxidant activity. The Folin-Ciocalteu method for determining the total phenolic amount and the AlCl3 method for the total flavonoid content of the extract was chosen. Mean diameters of inhibition zones (IZD) of the bacteria were found in the range of 8.2 mm to 24.45 mm. This value was measured as 17.82 mm and 18.69 mm for yeasts. The total antioxidant activity value of the extract was calculated as 7.08 mg AAE/g extract. The IC50 value was found as 9.097 mg/mL for DPPH free radical scavenging activity. The FRAP value indicated that the reducing power of 1 gram of sample was equivalent to 4.66 μmol of Trolox. The total phenolic content of ethanol extract of R. gracilis rhizomes was determined as 0.414 mg GAE/g, while the flavonoid content was calculated as 0.68 mgQE/g. The analyzed ethanolic extract of R. gracilis rhizomes demonstrated that the biological activity level could be considered significant according to the obtained results.
Su Ürünlerinde Kullanılan Paketleme Yöntemleri: Vakum Paketleme, MAP, Biosensörler, Oksijen Emici Etiketler
Authors: ERDEM Mehmet Emin , TUNÇTAŞ Nuri , KÖSTEKLİ Bayram , KESKİN İrfan
Published: May 2022

Gıdaların muhafaza edilmesinde ve sağlıklı olarak tüketiciye ulaştırılmasında kullanılan paketleme malzemesinin büyük önemi vardır. Ambalajların en önemli özellikleri; üretim yeri, içeriği, üretim tarihi ve menşei gibi birçok bilgi konusunda tüketiciyi aydınlatmasıdır. Su ürünleri avlandığı andan itibaren, karşılaştığı tüm fiziksel ve çevresel faktörlerden etkilendiği için çok çabuk bozulmaktadır. Bunun için de en kısa sürede tüketilmeli, uygun koşullarda işlenmeli veya paketlenerek tüketiciye en iyi kalitede ulaştırılmalıdır. Hızla gelişen su ürünleri sektöründe, akıllı paketleme, akıllı etiketler, biosensörler, vakum ve modifiye ambalaj teknikleri ile oksijen emici etiketler de geliştirilmiştir. Mikrobiyolojik kalite ölçüm etiketleri, pH sensörleri, radyo frekansı tanımlama teknolojisi (RFİD), karekod uygulamaları ile ürünlerin pazarlama sonrası takibi de sağlanmaktadır. Bu çalışmada; su ürünleri paketleme yöntemleri;oksijen emici etiketler, RFİD ve biosensörlerin kullanım alanları hakkında bilgi verilmesi amaçlanmıştır.
Cloning and Determination of Kinetic Activity of Thermophilic GCH-I from Anoxybacillus flavithermus DSM 2641T
Authors: BERİŞ Fatih Şaban , HIZAL Özlem , KARAOĞLU Hakan
Published: May 2022

Phenylketonuria (PKU) is a disease caused by phenylalanine hydroxylase enzyme deficiency in newborn infants and is the most important cause of treatable mental retardation. One of the causes of the disease comes from the defects of the PTPS in the metabolic pathway of phenilalanine metabolisms. Treatment of the disease is not feasible, and life-time tetrahydrobiopterin loading is performed in chronic patients. Today, tetrahydrobiopterin is chemically synthesized. Biological production is a different point of view due to the long duration of chemical synthesis, costs, and exposure to chemical pollutants. For this reason, the gch-I gene from the thermophilic A. flavithermus DSM 2641T was identified by PCR method. We cloned the gchI gene that was 603 bp and its open reading frame has 200 amino acids. The gene was cloned into pET28a(+) expression vector with 6xHis tags and transform in E. coli BL21(DE3)pLys host cells to express with 1 mM IPTG induction. After purification with Ni-NTA resin, we determined that GCH-I is 24 kDa, its optimum pH is 8.0 and temperature is 65C.Under optimal conditions, GCH-I exhibited enzymatic activity with Km- and Vmax- values of 243 ± 23,25 μM and 100,93 ± 3,5 nM/min/mg protein, respectively.
Samsun İlinde Bulunan Gıcı, Tatlı, Balık ve Uzun Göllerindeki Balıkçılığın İncelenmesi
Authors: KULAK Turgut , ERDOĞAN SAĞLAM Naciye
Published: May 2022

Bu çalışma, 2019-2020 yılları arasında Samsun ilinde bulunan Gıcı, Tatlı, Balık ve Uzun göllerinde yürütülmüştür. S.S. Yörükler Su Ürünleri Kooperatiflerinin kurulduğu yıl, avlanma alanları, balıkçı sayısı, tekne sayısı, balıkçıların kullandığı teknelerin özellikleri, av araçları, avlanan su ürünleri miktarı, türleri ve balıkçıların sosyo-demografik özelliklerine yönelik veriler belirlenmiştir.
Çalışma sonucunda; Samsun Bölgesi’nde araştırma yapılan göllerde S.S. Yörükler Su Ürünleri Kooperatifi’ne bağlı 400 kayıtlı üye ve 34 kayıtlı teknenin bulunduğu tespit edilmiştir. Avlanan toplam su ürünleri miktarının 176.502 ton/yıl olduğu, su ürünleri avcılığında kullanılan ağ uzunluğunun 26400 m ve pinter ağlarının 1020 adet olduğu tespit edilmiştir.
Electron Cloud Densitometry of Inner and Valence Electrons in Carbon Allotropes
Authors: Kucherov Olexandr
Published: May 2022

Electron cloud densitometry of carbon allotropes is presented in this study. Carbon consists of two inner and four valence electrons. The valence electrons in carbon are hybridized or active. Each active valence electron builds a negatively charged shape due to their delocalization. It is proved that the active valence electron creates the van der Waals force, which bonds layers of crystalline graphite together. An easy quantum mechanical explanation of the electron cloud densitometry is given in this study. In accordance with this effect, an atom begins to illuminate, depicting its shape. Electron cloud densitometry images show the inner and valence electrons in the applied functional materials, such as activated coke, graphite, graphene, and diamond.
Motivasi, tantangan, dan mekanisme koping pekerja migran perempuan asal Sulawesi Utara di Hong Kong: Tulang rusuk yang menjadi tulang punggung
Authors: Supit Alva , Chan Marco
Published: May 2022

Pekerja migran perempuan Indonesia (PMPI) memiliki peranan amat penting dalam meningkatkan devisa negara Indonesia. Saat ini terdapat sekitar puluhan ribu PMPI di Hong Kong, 2.191 di antaranya berasal dari Sulawesi Utara. Sampai saat ini belum ada laporan khusus yang meneliti tentang keadaan PMPI asal Sulawesi Utara secara spesifik, terutama dari sudut pandang psikologis. Penelitian  ini bertujuan untuk mengetahui motivasi, tantangan, dan mekanisme koping para PMPI asal Sulawesi Utara dengan menggunakan metode penelitian kualitatif berupa wawancara, sintesis, dan triangulasi. Secara umum, mayoritas PMPI Sulawesi Utara di Hong Kong merasa puas dengan keberadaan mereka di Hong Kong, terutama dari segi penggajian dan perlakuan majikan. Tantangan yang dialami justru kebanyakan berasal dari keluarga di Indonesia. Terlepas dari berbagai tantangan yang dihadapi, mereka tetap bertahan di Hong Kong karena mereka adalah sumber nafkah utama bagi keluarga inti dan keluarga luas mereka. Mekanisme koping yang diambil untuk bertahan kebanyakan berupa sugesti diri dan berdoa, dan melakukan kegiatan keagamaan bersama teman-teman PMPI lainnya.
Detection and Localization of Image and Video Tampering
Authors:   Research Scholar,,CSE , Noorul Islam Centre for Higher Education,Kumarakovil, India., Joseph* Silpa , kumar S.Palani ,   Associate Professor ,department of IT, Noorul Islam Centre for Higher Education,Kumarakovil, India.
Published: May 2022

Digital images and videos are widely used in various fields like courtrooms ,military ,medicine ,research, social media etc. So, maintaining the authenticity and integrity of these digital contents is a major concern. For the past few years, researches were going on to find out tampering in the digital contents. Active and passive approaches are the major classification for digital tampering detection. Here, we discuss some of the active and passive approaches and their significance in the current scenario.
Disease Inference from Health-Related Questions using Sparse Neural Network and LSTM
Authors:   Assistant Professor, Department of Computer Science, Kristu Jayanti College, Bengaluru, India., Dhanamalar* Ms. M. , K Dr. Kavitha. ,   Assistant Professor, Mother Teresa Women's University, Kodaikanal, Tamil Nadu, India.
Published: May 2022

There are many relationship and context retrieval related problems that are resolved using medical records with Deep learning-based techniques, but when it comes to community data such as health care forums where the quality of data cannot be met as health records due to gap in medical vocabulary it becomes impossible to provide an accurate solution, our research involves in providing a solution to this problem. The graph algorithms have always provided the best solutions to map and normalize in NLP domain, we have used the same to find the normalized medical terms for out user questions in the health care forums. Instead of training the actual data from the forums with the LSTM, we create medical signatures of the words coming together to form a context in the medical dictionary. We consider the words used in the dataset as vertices and find dense subgraphs to uniquely identify the condition with medical dictionary data. In simple words, we aim to build a system to convert the vague descriptions of the disease to match the accurate medical term from the medical dictionary such as snomedCT. We used the words which co-occur to define our relations which will, in turn, provide us with a solution to bridge the gap of medical vocabulary. The mappings of normalized terms are foundations to build the hidden layer of our neural networks, instead of constructing a direct connection between the input neurons to all hidden neurons we connect only the subgraph results thus improving our accuracy to a better level then existing methodologies.
Strategi Pemasaran Produk Kopi Bubuk Mondong Dusun Buntu Riri Desa Buntu Mondong Kecamatan Buntu Batu Kabupaten Enrekang
Authors: Hasriani Hasriani , Nurhapsah Nurhapsa , Yusriadi Yusriadi
Published: May 2022

Kabupaten Enrekang dibudidayakan kopi pengembangan di Desa Buntu Mondong Kecamatan Buntu Batu yang menghasilkan kopi yang merupakan potensi yang dapat dikembangkan untuk meningkatkan kesejahteraan petani kopi. Tujuan dari penelitian ini adalah untuk mengetahui factor-faktor internal (kekuatan dan kelemahan) dan factor-faktor eksternal (peluang dan ancaman) dalam Usaha Mandiri Kopi Mondong serta untuk menentukan strategi yang tepat untuk Pemasaran Produk Kopi Bubuk Mondong Di Usaha Mandiri Kopi Mondong. Adapun metode analisis yang digunakan adalah Analisis SWOT. Dari hasil penelitian menunjukkan bahwa factor internal dan eksternal berdasarkan pembobotan dan pemberian rating diperoleh skor untuk factor kekuatan utama di Usaha Mandiri Kopi Mondong ini adalah kualitas produk yang baik dan kelemahannya yaitu promosi produksi masih lemah, sedangkan untuk factor peluang paling mendukung pada pemasaran pada perusahaan ini adalah produksi kopi yang meningkat dan factor ancaman yang akan dihadapi adalah daerah distribusi pesaing yang luas. Strategi yang sebaiknya diterapkan pada Usaha Mandiri Kopi Mondong Di Desa Buntu Mondong Kecamatan Buntu Batu Kabupaten Enrekang ini berdasarkan matriks SWOT adalah strategi strength-opportunities (SO) yaitu berupaya menggunakan kekuatan utamanya yaitu kualitas produk yang baik sehingga memperoleh peluang utamanya yaitu dapat bersaing dengan produk lain.
Enrekang Regency cultivated coffee development in Buntu Mondong Village, Buntu Batu District which produces coffee which is a potential that can be developed to improve the welfare of coffee farmers. The purpose of this study was to determine the internal factors (strengths and weaknesses) and external factors (opportunities and threats) in the Mandiri Kopi Mondong Business and to determine the right strategy for Marketing Mondong Coffee Powder Products at the Mandiri Kopi Mondong Business. The analysis method used is SWOT analysis. The results of the study indicate that internal and external factors based on weighting and rating are obtained for the main strength factor in Usaha Mandiri Kopi Mondong, which is good product quality and the weakness is that production promotion is still weak, while the opportunity factor is the most supportive of marketing at this company. is the increasing coffee production and the threat factor to be faced is the wide distribution area of competitors. The strategy that should be applied to the Mondong Coffee Independent Business in Buntu Mondong Village, Buntu Batu District, Enrekang Regency, based on the SWOT matrix is a strength-opportunities (SO) strategy, which is trying to use its main strength, namely good product quality so that it gets the main opportunity, namely being able to compete with other products.
Start-Up Potensial Melalui Experiential Learning
Authors: Nurliani Nurliani , Rosada Ida , Sabahannur St. , Amri A. Azrarul
Published: May 2022

Program Merdeka Belajar Kampus Merdeka (MBKM) sebagai langkah awal strategi kebijakan penyelenggaraan pendidikan dalam memfasilitasi potensi mahasiswa sehingga mampu membentuk karakter profesional dalam bidang kerja yang ditekuni nantinya. Program MBKM, jalur wirausaha memberikan ruang kepada mahasiswa yang memiliki minat dan komitmen untuk menjadi wirausaha mandiri, adaptif, kreatif dan inovatif. Penelitian ini bertujuan mengetahui minat wirausaha mahasiswa, karakteristik wirausaha, dan mendeskripsikan kompetensi wirausaha mahasiswa, yaitu aspek afektif, kognitif, dan psikomotorik. Penelitian ini merupakan penelitian survey, teknik pengambilan sampel adalah purposive sampling dengan jumlah sampel sebanyak 77 mahasiswa semester III Program Studi Agroteknologi dan Agribisnis, Universitas Muslim Indonesia. Analisis data menggunakan analisis deskriptif kuantitatif. Hasil penelitian menunjukkan bahwa minat mahasiswa mengikuti program MBKM sangat tinggi, yaitu 98.70%, karakteristik wirausaha mahasiswa termasuk kategori berkarakter dengan skor rata-rata 3.99. Kompetensi wirausaha mahasiswa berdasarkan indikator kemampuan kognitif, afektif, dan psikomotorik termasuk kategori memiliki kompetensi wirausaha dengan skor rata-rata 4.02.
The Independent Learning Campus Independent Program (MBKM) is the first step in the policy of implementing education in facilitating the potential of students so that they are able to form a professional character in the field of work that they will be engaged in later.  The MBKM program, the entrepreneurial pathway, provides space for students who have an interest and commitment to become independent, adaptive, creative and innovative entrepreneurs.This study aims to determine student entrepreneurial interests, entrepreneurial characteristics, and describe student entrepreneurial competencies, namely affective, cognitive, and psychomotor aspects.  This research is a survey research, the sampling technique is purposive sampling with a total sample of 77 third semester students of Agrotechnology and Agribusiness Study Program, Muslim University of Indonesia.  Data analysis using quantitative descriptive analysis. The results showed that the student's interest in participating in the MBKM program was very high, namely 98.70%, the entrepreneurial characteristics of students were included in the character category with an average score of 3.99.  Student entrepreneurial competence based on indicators of cognitive, affective, and psychomotor abilities is included in the category of having entrepreneurial competence with an average score of 4.02.
Pengaruh Penerapan Teori Keperawatan “Health Education” Terhadap Peningkatan Prespektif Masyarakat Terkait Covid-19 Di Wilayah Kerja Kelurahan Bangkala Kota Makassar
Authors: Ernawati Ernawati , Darwis Darwis , Mutmainna Amriati , Isa Wa Mina La , Mato Rusni , Askar M.
Published: May 2022

COVID-19 adalah suatu penyakit infeksi yang disebabkan oleh virus yang bernama SARS-CoV-2(Servere Acute Respiratory Syndrome Coronavirus-2). Pertama kali penyakit ini ditemukan di Kota Wuhan Provinsi Hubei, China pada akhir Desember 2019. Negara yang melaporkan kasus COVID-19 semakin banyak, sehingga menyebar semakin luas sampai menjangkiti 5 benua, maka dari itu pada tangga 11 Maret 2020 WHO menyatakan kasus tersebut sebagai pandemik. Secara Internasional, Senin, 7 Juni 2021 terdapat 173.005.553 kasus yang dikonfirmasi COVID-19, termasuk 3.727.605 kematian yang dilaporkan kepada WHO. Di Asia Tenggara sebanyak 32.772.064 terkonfirmasi COVID-19. Untuk Sulawesi Selatan per tanggal 7 Juni 2021 kasus terkonfirmasi COVID-19 sebanyak 62.378 orang dengan rincian 61010(97,8%) pasien sembuh dan 949(1,5%) pasien meninggal. Permasalahan yang dibahas dalam penelitian ini adalah “apakah ada pengaruh Health Education Terhadap Peningkatan Prespektif Masyarakat terkait COVID-19”. Berdasarkan   permasalahan tersebut maka penelitian ini bertujuan untuk mengetahui Penerapan Health Education Terhadap Prespektif Masyarakat terkait COVID-19 terhadap perbaikan prespektif masyarakat. Penelitian ini menggunakan pendekatan kualitatif dengan metode Quasi Experimental design. Teknik pengumpulan data dengan menggunakan kuesioner prespektif ) yang dipilih secara purposive sampling. Hasil penelitian menunjukan bahwa ada pengaruh Health education Terhadap Peningkatan Prespektif Masyarakat terkait COVID-19.
COVID-19 is an infectious disease caused by a virus called SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus-2). This disease was the first discovered in Wuhan City, Hubei Province, China at the end of December 2019. Increasingly countries have reported cases of COVID-19 otherwise it has spread more widely to infect 5 continents, therefore on March 11, 2020 WHO declared the case a pandemic. Internationally, Monday, 7 June 2021 were 173,005,553 confirmed cases of COVID-19, including 3,727,605 deaths reported from WHO. In Southeast Asia, 32,772,064 were confirmed to have COVID-19. For South Sulawesi as of June 7, 2021, there were 62,378 confirmed cases of COVID-19, with details of 61010 (97.8%) patients recovering and 949 (1.5%) patients dying. The problem discussed in this study "is there an effect of health education on increasing community perspectives related to COVID-19". Based on these problem, this study aimed to determine the application of health education to the community's perspective related to COVID-19 towards improving the community's perspective.This study used a quantitative approach with a quasi-experimental design method. The data collection technique used a perspective questionnaire which was selected by purposive sampling. The results of the study uncloaked there is an effect of health education on increasing community perspectives related to COVID-19.