bluuebunny/crossref_metadata_2025 huggingface dataset:
Current status and future directions of nanovaccine for cancer: a bibliometric analysis during 2004-2023
Authors: Hou Yuhui , Li Yue , Zhang Youao , Zhang Juan , Wu Dinglan
Published: July 2024

Background

Nanovaccine treatment is an exciting area of research in immunology and personalized medicine, holding great promise for enhancing immune responses and targeting specific diseases. Their small size allows efficient uptake by immune cells, leading to robust immune activation. They can incorporate immune-stimulating molecules to boost vaccine efficacy. Therefore, nanovaccine can be personalized to target tumor-specific antigens, activating the immune system against cancer cells. Currently, there have been ample evidence showing the effectiveness and potential of nanovaccine as a treatment for cancer. However, there was rare bibliometric analysis of nanovaccine for cancer. Here we performed a bibliometric and visual analysis of published studies related to nanovaccine treatment for cancer, providing the trend of future development of nanovaccine.

Methods

We collected the literatures based on the Web of Science Core Collection SCI-Expanded database. The bibliometric analysis was performed via utilizing visualization analysis tools VOSviewer, Co-Occurrence (COOC), Citespace, Bibliometrix (R-Tool of R-Studio), and HitCite.

Results

A total of 517 literatures were included in this study. China is the country with the most publications and the highest total local citation score (TLCS). The Chinese Academy of Sciences holds the largest research count in this field and the most prolific author is Deling Kong from Nankai University. The most prominent journal for publishing in this area is Biomaterials. The researches mainly focus on the therapeutic process of tumor nanovaccines, the particle composition and the application of nanovaccines, suggesting the potential hotspots and trends of nanovaccine.

Conclusion

In this study, we summarized the characteristics and variation trends of publications involved in nanovaccine, and categorized the most influential countries, institutions, authors, journals, hotspots and trends regarding the nanovaccine for cancer. With the continuous development of nanomaterials and tumor immunotherapy, nanovaccine for cancer provides a research field of significant clinical value and potential application.
Regulatory T cells and immune escape in HCC: understanding the tumor microenvironment and advancing CAR-T cell therapy
Authors: Du Guangtan , Dou Cunmiao , Sun Peng , Wang Shasha , Liu Jia , Ma Leina
Published: July 2024

Liver cancer, which most commonly manifests as hepatocellular carcinoma (HCC), is the sixth most common cancer in the world. In HCC, the immune system plays a crucial role in the growth and proliferation of tumor cells. HCC achieve immune escape through the tumor microenvironment, which significantly promotes the development of this cancer. Here, this article introduces and summarizes the functions and effects of regulatory T cells (Tregs) in the tumor microenvironment, highlighting how Tregs inhibit and regulate the functions of immune and tumor cells, cytokines, ligands and receptors, etc, thereby promoting tumor immune escape. In addition, it discusses the mechanism of CAR-T therapy for HCC and elaborate on the relationship between CAR-T and Tregs.
The effects of inflammation on connexin 43 in chronic Chagas disease cardiomyopathy
Authors: Barreto Breno Cardim , Neves Maria Vitória Gomes das , Cardoso Carine Machado Azevedo , Meira Cássio Santana , Daltro Pâmela Santana , Figueira Cláudio Pereira , Santos Girlaine Café , Silva Daniela Nascimento , Távora Fábio , Neto João David de Souza , Macambira Simone Garcia , Lampe Paul D. , Coutinho Keyla Cristiny da Silva , Kasai Brunswick Tais Hanae , Ribeiro dos Santos Ricardo , Campos de Carvalho Antônio Carlos , Soares Milena Botelho Pereira
Published: July 2024

Background

Cardiac arrhythmias are the main cause of sudden death due to Chronic Chagasic Cardiomyopathy (CCC). Here we investigated alterations in connexin 43 (Cx43) expression and phosphorylation in cardiomyocytes as well as associations with cardiac arrhythmias in CCC.

Methods

C57Bl/6 mice infected with Trypanosoma cruzi underwent cardiac evaluations at 6 and 12 months after infection via treadmill testing and EKG. Histopathology, cytokine gene expression, and distribution of total Cx43 and its phosphorylated forms Cx43S368 and Cx43S325/328/330 were investigated. Human heart samples obtained from subjects with CCC were submitted to immunofluorescence analysis. In vitro simulation of a pro-inflammatory microenvironment (IL-1β, TNF, and IFN-γ) was performed in H9c2 cells and iPSC-derived cardiomyocytes to evaluate Cx43 distribution, action potential duration, and Lucifer Yellow dye transfer.

Results

Mice chronically infected with T. cruzi exhibited impaired cardiac function associated with increased inflammation, fibrosis and upregulated IL-1β, TNF, and IFN-γ gene expression. Confocal microscopy revealed altered total Cx43, Cx43S368 and Cx43S325/328/330 localization and phosphorylation patterns in CCC, with dispersed staining outside the intercalated disc areas, i.e., in lateral membranes and the cytoplasm. Reduced co-localization of total Cx43 and N-cadherin was observed in the intercalated discs of CCC mouse hearts compared to controls. Similar results were obtained in human CCC heart samples, which showed Cx43 distribution outside the intercalated discs. Stimulation of human iPSC-derived cardiomyocytes or H9c2 cells with IL-1β, TNF, and IFN-γ induced alterations in Cx43 localization, reduced action potential duration and dye transfer between adjacent cells.

Conclusion

Heart inflammation in CCC affects the distribution and phosphorylation pattern of Cx43, which may contribute to the generation of conduction disturbances in Chagas disease.
The European Ocean Observing Community: urgent gaps and recommendations to implement during the UN Ocean Decade
Authors: Hassoun Abed El Rahman , Tanhua Toste , Lips Inga , Heslop Emma , Petihakis George , Karstensen Johannes
Published: July 2024

The interdisciplinary community of ocean and coastal observers and modelers in Europe is driven by national observing needs for prediction and management of intricate processes shaping Europe’s Seas. Not all observing needs can be addressed by nations alone and various coordination activities exist to overcome fragmentation and create cross benefit within the European Ocean Observing Community (EOOC). This way critical insights into impacts of climate change on European Waters and cross border marine resources management can be achieved. Based on a large number of published material, this article is identifying and addressing the current state of activities of the EOOC and states gaps that potentially prevent efficacy. Key challenges include spatial and temporal coverage in observations, data integration, accessibility, uncertainties in projections, technological hurdles, and engagement and communication gaps. Detailed recommendations are provided for identified gaps, offering valuable insights for stakeholders, funders, and supporters of the EOOC. These recommendations, extending beyond academic interest, carry significant implications for climate change mitigation, marine resource management efficiency, ecosystem resilience, disaster preparedness, economic benefits, and the broader scientific advancements in European marine science, thereby benefiting society at large. As the world undergoes transformative changes impacting all facets of European life, substantial investment and support for the EOOC are crucial for precise information, accurate predictions, supporting sustained services that contribute to business growth and community resilience, and a sustainable ocean.
Triangular fossa of the third cerebral ventricle – an original 3D model and morphometric study
Authors: Nedelcu Alin Horatiu , Lupu Vasile Valeriu , Lupu Ancuta , Tepordei Razvan Tudor , Ioniuc Ileana , Stan Cristinel Ionel , Vicoleanu Simona Alice Partene , Haliciu Ana Maria , Statescu Gabriel , Ursaru Manuela , Danielescu Ciprian , Tarniceriu Cristina Claudia
Published: July 2024

Introduction

The triangular recess (TR), also called triangular fossa or vulva cerebri, represents the anterior extension of the diencephalic ventricle, located between the anterior columns of the fornix and the anterior white commissure. Over time, this structure of the third cerebral ventricle generated many disputes. While some anatomists support its presence, others have opposite opinions, considering that it only becomes visible under certain conditions. The aim of the study is to demonstrate the tangible structure of the triangular recess. Secondly, the quantitative analysis allowed us to establish an anatomical morphometric standard, as well as the deviations from the standard.

Materials and methods

Our study is both a quantitative and a qualitative evaluation of the triangular fossa. We dissected 100 non-neurological adult brains, which were fixed in 10% formaldehyde solution for 10 weeks. The samples are part of the collection of the Institute of Anatomy, “Grigore T. Popa” University of Medicine and Pharmacy, Iasi. We highlighted the triangular fossa by performing dissections in two stages at the level of the roof of the III ventricle.

Results

The qualitative analysis is a re-evaluation of the classical data concerning the anatomy of the fossa triangularis. We proposed an original 3D model of the triangular recess in which we described a superficial part called vestibule and a deep part called pars profunda. We measured the sides of the communication between the two proposed segments, as well as the communication with the III ventricle. By applying the Heron’s formula, we calculated the area of the two communications. Statistical evaluations have shown that these communications are higher than they are wide. In addition, there is a statistical difference between the surfaces of the two communications: 34.07 mm2 ± 7.01 vs. 271.43 mm2 ± 46.36 (p = 0.001).

Conclusion

The outcome of our study is both qualitative and quantitative. Firstly, we demonstrated the existence of the triangular fossa and we conceived a spatial division of this structure. Secondly, the measurements carried out establish an anatomo-morphometric norm of the triangular recess, which is useful in assessing the degree of hydrocephalus during the third endoscopic ventriculoscopy.
Machine learning-based gait adaptation dysfunction identification using CMill-based gait data
Authors: Yang Hang , Liao Zhenyi , Zou Hailei , Li Kuncheng , Zhou Ye , Gao Zhenzhen , Mao Yajun , Song Caiping
Published: July 2024

Background

Combining machine learning (ML) with gait analysis is widely applicable for diagnosing abnormal gait patterns.

Objective

To analyze gait adaptability characteristics in stroke patients, develop ML models to identify individuals with GAD, and select optimal diagnostic models and key classification features.

Methods

This study was investigated with 30 stroke patients (mean age 42.69 years, 60% male) and 50 healthy adults (mean age 41.34 years, 58% male). Gait adaptability was assessed using a CMill treadmill on gait adaptation tasks: target stepping, slalom walking, obstacle avoidance, and speed adaptation. The preliminary analysis of variables in both groups was conducted using t-tests and Pearson correlation. Features were extracted from demographics, gait kinematics, and gait adaptability datasets. ML models based on Support Vector Machine, Decision Tree, Multi-layer Perceptron, K-Nearest Neighbors, and AdaCost algorithm were trained to classify individuals with and without GAD. Model performance was evaluated using accuracy (ACC), sensitivity (SEN), F1-score and the area under the receiver operating characteristic (ROC) curve (AUC).

Results

The stroke group showed a significantly decreased gait speed (p = 0.000) and step length (SL) (p = 0.000), while the asymmetry of SL (p = 0.000) and ST (p = 0.000) was higher compared to the healthy group. The gait adaptation tasks significantly decreased in slalom walking (p = 0.000), obstacle avoidance (p = 0.000), and speed adaptation (p = 0.000). Gait speed (p = 0.000) and obstacle avoidance (p = 0.000) were significantly correlated with global F-A score in stroke patients. The AdaCost demonstrated better classification performance with an ACC of 0.85, SEN of 0.80, F1-score of 0.77, and ROC-AUC of 0.75. Obstacle avoidance and gait speed were identified as critical features in this model.

Conclusion

Stroke patients walk slower with shorter SL and more asymmetry of SL and ST. Their gait adaptability was decreased, particularly in obstacle avoidance and speed adaptation. The faster gait speed and better obstacle avoidance were correlated with better functional mobility. The AdaCost identifies individuals with GAD and facilitates clinical decision-making. This advances the future development of user-friendly interfaces and computer-aided diagnosis systems.
Establishment of the Norwegian hearing register for children
Authors: Mattsson Tone Stokkereit , Nilsen Ann Helen , Wennberg Siri
Published: July 2024

Introduction

The Norwegian Directorate of Health approved the Norwegian Hearing Register for Children in 2022. The main objective of the register is to improve the quality of treatment for children with permanent hearing loss, by measures, follow-ups and monitoring the quality and results of the health care system.

Methods

Inclusion criteria are children who do not pass universal newborn hearing screening and/or children with permanent hearing loss <18 years of age. Hearing loss is defined as pure-tone audiometry threshold of (PTA4) > 20 dB in at least one ear. Data are registered at the Ear, Nose and Throat departments at inclusion and at follow-ups at the age of 3, 6, 10, and 15 years. The register collects information about the child within a holistic perspective. The key elements of the register are (a) data concerning newborn hearing screening; (b) data concerning hearing, medical information, hearing amplification and intervention (c) patient reported outcome measures registered by caregivers using three questionnaires; Pediatric Quality of Life Inventory, Strengths and Difficulties Questionnaire and Parents’ Evaluation of Aural/Oral Performance of Children.

Results

The register has established four quality indicators regarding newborn hearing screening and early intervention (a) the rate of false positive neonatal screens; (b) testing for congenital cytomegalovirus within 3 weeks of age for children who do not pass newborn hearing screening; (c) audiological evaluation to confirm the hearing status no later than 3 months of age and (d) initiated intervention within 3 months after confirmation of hearing status.

Discussion

The register will include the total population of hearing impaired children over long time periods. Thus, the register enables each hospital to monitor their quality indicator scores continuously and compare them with national levels in real time. This facilitates and accelerates identification of improvement areas in the hospitals and will be an important contributor for quality improvement in NHS, diagnostics and hearing intervention for children in Norway. In addition, data from the register will be a unique source for research, and study designs with a long follow-up time can be applied.
Maternal vitamin D status during pregnancy and infant’s gut microbiota: a prospective cohort study
Authors: Song Qiying , Li Yao , Zhou Tao , Xiao Meiqun , Xiao Bin , Wang Mengying , Zhu Yuanfang
Published: July 2024

Objectives

To prospectively explore the association of maternal serum 25(OH)D levels with the infant’s gut microbiota in Chinese populations, and to evaluate its potential influence on the dynamic change patterns of offspring’s gut microbiota from 1 to 6 months old.

Methods

Eighty-seven mother-infant dyads (vitamin D insufficient group vs. normal group = 59 vs. 28) were included in this longitudinal study. Two fecal samples were collected for the included infant at home by the parents at 1 month of age (“M1 phase”) and 6 months of age (“M6 phase”). Gut microbiota were profiled by 16S rRNA gene sequencing. We performed mixed effects models on alpha diversity metrics, PERMANOVA tests on beta diversity distances, and linear discriminant analysis (LDA) to identify differently abundant taxa.

Results

We observed significantly lower Pielou’s evenness and Shannon diversity in the vitamin D insufficient group in the M6 phase (p = 0.049 and 0.015, respectively), but not in the M1 phase (p > 0.05), and the dynamic changes in alpha diversity from 1 to 6 months old were significantly different according to maternal vitamin D status (p < 0.05). There were also significant differences in gut microbiota composition between the vitamin D insufficient group and normal group, both in the M1 and M6 phases (LDA score > 2.0, p < 0.05). Moreover, among the predicted metagenome functions, pathways related to amino acid biosynthesis, starch degradation, and purine nucleotides biosynthesis were enriched in the vitamin D insufficient group.

Conclusion

Our findings highlight that maternal vitamin D status plays a pivotal role in shaping the early-life gut microbiota of the next generation.
A comparison of raspberry freezing-damage during preservation in isochoric and isobaric conditions
Authors: Câmpean Ștefan Ioan , Beșchea George Andrei , Vuțoiu Beatrice Georgiana , Tăbăcaru Maria Bianca , Năstase Gabriel
Published: July 2024

Introduction

Fruits are perishable, thus it’s crucial to have an efficient preservation technique that can increase storage time while keeping physical quality and nutritional attributes in order to avoid wastage. The majority of methods for long-term storage require refrigeration.

Methods

In this investigation, we assess the viability of isochoric freezing as a different technique of raspberry (Rubus idaeus L.) preservation. Raspberries were subjected to different storage conditions: isochoric freezing at –4°C, conventional isobaric settings at +3°C (refrigerator), –21°C (freezer), and –4°C with a trehalose solution in a plastic bag. The study assessed changes in weight loss, visual appearance, color, hardness, °Brix values, and pH over a seven-day period.

Results

Key findings reveal that raspberries subjected to isochoric freezing below the freezing point of water experienced minimal weight loss after seven days. Visual appearance, color, hardness, °Brix values, and pH were comparable to fresh raspberries, indicating minimal alteration.

Discussion

These results suggest that isochoric freezing shows potential as a preservation method that maintains the physical and chemical properties of raspberries similar to fresh fruit. Implementing diverse preservation techniques tailored to raspberries may contribute to environmental sustainability by reducing food wastage and the associated environmental impact.
PI-YOLO: dynamic sparse attention and lightweight convolutional based YOLO for vessel detection in pathological images
Authors: Li Cong , Che Shuanlong , Gong Haotian , Ding Youde , Luo Yizhou , Xi Jianing , Qi Ling , Zhang Guiying
Published: July 2024

Vessel density within tumor tissues strongly correlates with tumor proliferation and serves as a critical marker for tumor grading. Recognition of vessel density by pathologists is subject to a strong inter-rater bias, thus limiting its prognostic value. There are many challenges in the task of object detection in pathological images, including complex image backgrounds, dense distribution of small targets, and insignificant differences between the features of the target to be detected and the image background. To address these problems and thus help physicians quantify blood vessels in pathology images, we propose Pathological Images-YOLO (PI-YOLO), an enhanced detection network based on YOLOv7. PI-YOLO incorporates the BiFormer attention mechanism, enhancing global feature extraction and accelerating processing for regions with subtle differences. Additionally, it introduces the CARAFE upsampling module, which optimizes feature utilization and information retention for small targets. Furthermore, the GSConv module improves the ELAN module, reducing model parameters and enhancing inference speed while preserving detection accuracy. Experimental results show that our proposed PI-YOLO network has higher detection accuracy compared to Faster-RCNN, SSD, RetinaNet, YOLOv5 network, and the latest YOLOv7 network, with a mAP value of 87.48%, which is 2.83% higher than the original model. We also validated the performance of this network on the ICPR 2012 mitotic dataset with an F1 value of 0.8678, outperforming other methods, demonstrating the advantages of our network in the task of target detection in complex pathology images.
A comprehensive review on lncRNA LOXL1-AS1: molecular mechanistic pathways of lncRNA LOXL1-AS1 in tumorigenicity of cancer cells
Authors: Yousefnia Saghar
Published: July 2024

Long non-coding RNAs (lncRNAs) are versatile RNAs that regulate various cellular processes, such as gene regulation, by acting as signals, decoys, guides, and scaffolds. A novel recognized lncRNA, LOXL1-antisense RNA 1 (LOXL1-AS1), is dysregulated in some diseases, including cancer, and acts as an oncogenic lncRNA in many types of cancer cells. Upregulation of LOXL1-AS1 has been involved in proliferation, migration, metastasis, and EMT, as well as inhibiting apoptosis in cancer cells. Most importantly, the malignant promoting activity of LOXL1-AS1 can be mostly mediated by sequestering specific miRNAs and inhibiting their binding to the 3´UTR of their target mRNAs, thereby indirectly regulating gene expression. Additionally, LOXL1-AS1 can decoy transcription factors and proteins and prevent their binding to their regulatory regions, inhibiting their mechanistic activity on the regulation of gene expression and signaling pathways. This review presents the mechanistic pathways of the oncogenic role of LOXL1-AS1 by modulating its target miRNAs and proteins in various cancer cells. Having information about the molecular mechanisms regulated by LOXL1-AS1 in cancer cells can open ways to find out particular prognostic biomarkers, as well as discover novel therapeutic approaches for different types of cancer.
Gasdermin D could be lost in the brain parenchyma infarct core and a pyroptosis-autophagy inhibition effect of Jie-Du-Huo-Xue decoction after stroke
Authors: Zhou Chang , Qiu Shi-wei , Wang Feng-ming , Liu Yu-chen , Hu Wei , Yang Mei-lan , Liu Wang-hua , Li Hua
Published: July 2024

Background

The Chinese ethnic medicine Jie-Du-Huo-Xue Decoction (JDHXD) is used to alleviate neuroinflammation in cerebral ischemia (CI). Our previous studies have confirmed that JDHXD can inhibit microglial pyroptosis in CI. However, the pharmacological mechanism of JDHXD in alleviating neuroinflammation and pyroptosis needs to be further elucidated. New research points out that there is an interaction between autophagy and inflammasome NLRP3, and autophagy can help clear NLRP3. The NLRP3 is a key initiator of pyroptosis and autophagy. The effect of JDHXD promoting autophagy to clear NLRP3 to inhibit pyroptosis on cerebral ischemia-reperfusion inflammatory injury is currently unknown. We speculate that JDHXD can inhibit pyroptosis in CI by promoting autophagy to clear NLRP3.

Methods

Chemical characterization of JDHXD was performed using LC-MS. Model of middle cerebral artery occlusion/reperfusion (MCAO/R) was established in SD rats. Neurological deficits, neuron damage, and cerebral infarct volume were evaluated. Western Blot and immunofluorescence were used to detect neuronal pyroptosis and autophagy.

Results

30 possible substance metabolites in JDHXD medicated serum were analyzed by LC-MS (Composite Score > 0.98). Furthermore, JDHXD protects rat neurological function and cerebral infarct size after CI. JDHXD inhibited the expression of pyroptosis and autophagy after CI. Our western blot and immunofluorescence results showed that JDHXD treatment can reduce the expression of autophagy-related factors ULK1, beclin1, and LC3-Ⅱ. The expression of NLRP3 protein was lower in the JDHXD group than in the I/R group. Compared with the I/R group, the expressions of pyroptosis-related factors caspase-1 P 10, GSDMD-NT, IL-18, and IL-1β decreased in the JDHXD group. Furthermore, we observed an unexpected result: immunofluorescence demonstrated that Gasdermin D (GSDMD) was significantly absent in the infarct core, and highly expressed in the peri-infarct and contralateral cerebral hemispheres. This finding challenges the prevailing view that GSDMD is elevated in the ischemic cerebral hemisphere.

Conclusion

JDHXD inhibited pyroptosis and autophagy after MCAO/R. JDHXD suppressed pyroptosis and autophagy by inhibiting NLRP3, thereby alleviating CI. In addition, we present a different observation from previous studies that the expression of GSDMD in the infarct core was lower than that in the peri-infarct and contralateral non-ischemic hemispheres on day 3 of CI.
Underwater temperature and pressure monitoring for deep-sea SCUBA divers using optical techniques
Authors: Mirza Jawad , Kanwal Firdos , Salaria Umair Ahmad , Ghafoor Salman , Aziz Imran , Atieh Ahmad , Almogren Ahmad , Haq Anwar Ul , Kanwal Benish
Published: July 2024

The safety of SCUBA divers remains at high risk in deep-sea owing to multiple factors such as dangerous surrounding, rely upon technical equipment necessary for life support, decreased underwater navigation, and communication infrastructure. Gradual decrease and increase in water temperature and pressure corresponding to depth are among the most common problems that cause most of the fatalities in deep-sea diving. Therefore, different gadgets for accurate measurement of vital parameters, reliable navigation, and seamless communication are of prime importance. In this paper, we propose an all-optical technique for local and remote monitoring of underwater temperature and pressure for deep-sea SCUBA divers based on fiber Bragg grating (FBG) sensors and underwater optical communication-single mode fiber (UWOC-SMF) integrated transmission system. The proposed technique is implemented using two FBG temperature and pressure sensors fixed over diver’s suit and UWOC-SMF integrated transmission system for simultaneous local and remote monitoring of underwater temperature and pressure. Remote monitoring of underwater temperature and pressure is achieved at ship station through a remotely operated underwater vehicle (ROV) and UWOC-SMF integrated transmission system by means of shifts in the original Bragg wavelengths of sensors due to temperature and pressure variations. The performance of the sensors is analyzed for pressure and temperature in the range of 0 to 6.4 MPa (≈0 to 655 mH2O) and 40 to −2°C, respectively corresponding to different depths. The results show that the proposed technique can work well in the deep ocean over a range of pressures and temperatures of 0–7 MPa and 40 to −2°C while achieving a temperature sensitivity of 4.3 p.m./°C and a pressure sensitivity of 30.5 p.m./MPa. Clear spectra of reflected signals from FBG sensors at ship station are achieved after signal transmission over UWOC-SMF hybrid link.
Capturing physiological hemodynamic flow and mechanosensitive cell signaling in vessel-on-a-chip platforms
Authors: Martier A. , Chen Z. , Schaps H. , Mondrinos M. J. , Fang J. S.
Published: July 2024

Recent advances in organ chip (or, “organ-on-a-chip”) technologies and microphysiological systems (MPS) have enabledin vitroinvestigation of endothelial cell function in biomimetic three-dimensional environments under controlled fluid flow conditions. Many current organ chip models include a vascular compartment; however, the design and implementation of these vessel-on-a-chip components varies, with consequently varied impact on their ability to capture and reproduce hemodynamic flow and associated mechanosensitive signaling that regulates key characteristics of healthy, intact vasculature. In this review, we introduce organ chip and vessel-on-a-chip technology in the context of existingin vitroandin vivovascular models. We then briefly discuss the importance of mechanosensitive signaling for vascular development and function, with focus on the major mechanosensitive signaling pathways involved. Next, we summarize recent advances in MPS and organ chips with an integrated vascular component, with an emphasis on comparing both the biomimicry and adaptability of the diverse approaches used for supporting and integrating intravascular flow. We review current data showing how intravascular flow and fluid shear stress impacts vessel development and function in MPS platforms and relate this to existing work in cell culture and animal models. Lastly, we highlight new insights obtained from MPS and organ chip models of mechanosensitive signaling in endothelial cells, and how this contributes to a deeper understanding of vessel growth and functionin vivo. We expect this review will be of broad interest to vascular biologists, physiologists, and cardiovascular physicians as an introduction to organ chip platforms that can serve as viable model systems for investigating mechanosensitive signaling and other aspects of vascular physiology.
Heme (dys)homeostasis and liver disease
Authors: Duarte Tiago L. , Viveiros Nicole , Godinho Catarina , Duarte Delfim
Published: July 2024

Heme is essential for a variety of proteins involved in vital physiological functions in the body, such as oxygen transport, drug metabolism, biosynthesis of steroids, signal transduction, antioxidant defense and mitochondrial respiration. However, free heme is potentially cytotoxic due to the capacity of heme iron to promote the oxidation of cellular molecules. The liver plays a central role in heme metabolism by significantly contributing to heme synthesis, heme detoxification, and recycling of heme iron. Conversely, enzymatic defects in the heme biosynthetic pathway originate multisystemic diseases (porphyrias) that are highly associated with liver damage. In addition, there is growing evidence that heme contributes to the outcomes of inflammatory, metabolic and malignant liver diseases. In this review, we summarize the contribution of the liver to heme metabolism and the association of heme dyshomeostasis with liver disease.
Chlorophyll fluorescence, physiology, and yield of winter wheat under different irrigation and shade durations during the grain-filling stage
Authors: Naseer Muhammad Asad , Hussain Sadam , Mukhtar Ahmed , Rui Qian , Ru Guo , Ahmad Haseeb , Zhang Zhi Qin , Shi Li Bo , Asad Muhammad Shoaib , Chen Xiaoli , Zhou Xun Bo , Ren Xiaolong
Published: July 2024

The uneven spatial and temporal distribution of light resources and water scarcity during the grain-filling stage pose significant challenges for sustainable crop production, particularly in the arid areas of the Loess Plateau in Northwest China. This study aims to investigate the combined effects of drought and shading stress on winter wheat growth and its physio-biochemical and antioxidative responses. Wheat plants were subjected to different drought levels— full irrigation (I100), 75% of full irrigation (I75), 50% of full irrigation (I50), and 25% of full irrigation (I25), and shading treatments — 12, 9, 6, 3 and 0 days (SD12, SD9, SD6, SD3, and CK, respectively) during the grain-filling stage. The effects of drought and shading treatments reduced yield in descending order, with the most significant reductions observed in the SD12 and I25 treatments. These treatments decreased grain yield, spikes per plant, 1000-grain weight, and spikelets per spike by 160.67%, 248.13%, 28.22%, and 179.55%, respectively, compared to the CK. Furthermore, MDA content and antioxidant enzyme activities exhibited an ascending trend with reduced irrigation and longer shading durations. The highest values were recorded in the I75 and SD12 treatments, which increased MDA, SOD, POD, and CAT activities by 65.22, 66.79, 65.07 and 58.38%, respectively, compared to the CK. The Pn, E, Gs, and iCO2 exhibited a decreasing trend (318.14, 521.09, 908.77, and 90.85%) with increasing shading duration and decreasing irrigation amount. Drought and shading treatments damage leaf chlorophyll fluorescence, decreasing yield and related physiological and biochemical attributes.
Three novel QTLs for FHB resistance identified and mapped in spring wheat PI672538 by bulked segregant analysis of the recombinant inbred line
Authors: Huang Qianglan , Li Xin , Li Qing , Zhong Shengfu , Li Xiuying , Yang Jiezhi , Tan Feiquan , Ren Tianheng , Li Zhi , Suizhuang Yang
Published: July 2024

Introduction

Fusarium head blight (FHB) has a large influence on both the yield and quality of wheat grain worldwide. Host resistance is the most effective method for controlling FHB, but unfortunately, very few genetic resources on FHB resistance are available; therefore, identifying novel resistance genes or quantitative trait loci (QTLs) is valuable.

Methods

Here, a recombinant inbred line (RIL) population containing 451 lines derived from the cross L661/PI672538 was sown in four different environments (2019CZa, 2019CZb, 2021QL and 2021WJ).

Results

Five QTLs, consisting of two previously reported QTLs (FhbL693a and FhbL693b) and three new QTLs (FhbL693c, FhbL693d and FhbL693e), were identified. Further investigation revealed that FhbL693b, FhbL693c and FhbL693d could be detected in all four environments, and FhbL693a and FhbL693e were detected only in 2019CZb and 2021WJ, respectively. Among the QTLs, the greatest contribution (10.5%) to the phenotypic variation effect (PVE) was FhbL693d in 2021WJ, while the smallest (1.2%) was FhbL693e and FhbL693a in 2019CZb. The selection of 5Dindel-4 for FhbL693d, 4Aindel-7 for FhbL693c and 3Bindel-24 for FhbL693b decreased the number of damaged spikelets by 2.1, and a new line resistant to FHB named H140-2 was developed by marker-assisted selection (MAS).

Discussion

These results could help to further improve FHB resistance in the future.
Assessing the impact of climate warming on tree species composition and distribution in the forest region of Northeast China
Authors: Fu Yuanyuan , Liu Chang , He Hong S. , Wang Shaoqiang , Wang Lunche , Xie Zhijie
Published: July 2024

Global climate change has markedly influenced the structure and distribution of mid-high-latitude forests. In the forest region of Northeast China, the magnitude of climate warming surpasses the global average, which presents immense challenges to the survival and habitat sustainability of dominant tree species. We predicted the potential changes in aboveground biomass, dominant tree species composition, and distribution in the forest region of Northeast China over the next century under different climatic conditions encompassing the current scenario and future scenarios (RCP2.6, RCP4.5, and RCP8.5). Forest ecosystem process model LINKAGES 3.0 was used to simulate dynamic changes in species-level aboveground biomass under four climate scenarios at the homogeneous land-type unit level. The potential spatial distribution of tree species was investigated based on three indicators: extinction, colonization, and persistence. The results showed that LINKAGES 3.0 model effectively simulated the aboveground biomass of 17 dominant tree species in the forest region of Northeast China, achieving a high accuracy with R² = 0.88. Under the current, RCP2.6, and RCP4.5 climate scenarios, the dominant tree species presented gradual increases in aboveground biomass, whereas under RCP8.5, an initial increase and subsequent decline were observed. With increasing warming magnitude, cold-temperate coniferous tree species will gradually be replaced by other temperate broad-leaved tree species. Furthermore, a large temperature increase under RCP8.5 will likely produce a significant contraction in the potential distribution range of tree species like Larch, Scotch pine, Ribbed birch, Spruce and Fir, while most temperate broad-leaved tree species and Korean pine are expected to demonstrate a northward migration. These findings provide guidance for enhancing the adaptability and resilience of forest ecosystems in middle and high latitudes and addressing the threats posed by climate warming.
Assessing service usage and protective factors in a pediatric psychiatry clinic during the COVID-19 pandemic
Authors: Caruso A. J. , Basu A. , Urban T. H. , Kaskas M. M. , Rotter N. , Wozniak J. , Friedman D.
Published: July 2024

Youth with developmental and pre-existing mental health conditions have been particularly vulnerable to declines in psychological functioning during the COVID-19 pandemic. This study aimed to first, analyze service usage within an outpatient child and adolescent psychiatry clinic in the months preceding and during the COVID-19 pandemic, and second, to examine associations with potential protective factors against mental health concerns in a treatment-engaged sample. Service usage was examined using clinic billing data, and reports on protective factors were gathered via parent survey of 81 children ages 6–17 years who received mental health treatment in an outpatient psychiatry clinic during the pandemic. Protective factors were assessed at the individual, family, and community levels, and included children’s use of coping strategies, parental resilience, and parents’ perceived social supports. Study outcomes, including mental health concerns, mental health emergencies, pandemic-related distress, and social impact of the pandemic, were analyzed via Pearson correlations and simultaneous multiple linear regressions. Findings suggest increased service usage and child coping, parental resilience, and social connectedness as factors associated with fewer mental health concerns in youth with psychiatric concerns during the pandemic. This study lends support for expanding psychiatric services with continued use of telemedicine platforms. Further, findings suggest a mental health benefit to optimizing individual, parental, and community-based resources to enhance children’s psychological functioning, particularly for youth with pre-existing mental health conditions.
Emotional responses of Korean and Chinese women to Hangul phonemes to the gender of an artificial intelligence voice
Authors: Lee Min-Sun , Lee Gi-Eun , Lee San Ho , Lee Jang-Han
Published: July 2024

Introduction

This study aimed to explore the arousal and valence that people experience in response to Hangul phonemes based on the gender of an AI speaker through comparison with Korean and Chinese cultures.

Methods

To achieve this, 42 Hangul phonemes were used, in a combination of three Korean vowels and 14 Korean consonants, to explore cultural differences in arousal, valence, and the six foundational emotions based on the gender of an AI speaker. A total 136 Korean and Chinese women were recruited and randomly assigned to one of two conditions based on voice gender (man or woman).

Results and discussion

This study revealed significant differences in arousal levels between Korean and Chinese women when exposed to male voices. Specifically, Chinese women exhibited clear differences in emotional perceptions of male and female voices in response to voiced consonants. These results confirm that arousal and valence may differ with articulation types and vowels due to cultural differences and that voice gender can affect perceived emotions. This principle can be used as evidence for sound symbolism and has practical implications for voice gender and branding in AI applications.
Over-specification of small, borderline cardinalities and color in referential communication: the role of visual context, modifier position, and consistency
Authors: Zevakhina Natalia A. , Dongarova Kseniya N. , Shubina Daria , Popova Daria P.
Published: July 2024

This paper reports on two flash-mode experiments that test redundant descriptions of small (2–4) cardinalities, borderline (5–8) cardinalities, and color in referential communication. It provides further support for the idea that small cardinalities are more salient (due to subitizing), less sensitive to visual context, and therefore give rise to higher over-specification rates than color. Because of greater salience, Russian speakers more often use prenominal positions for numerals than for color adjectives. The paper also investigates borderline cardinalities and argues for the order factor that affects their salience, since ordered items can be perceived in small subitized parts. The ordered mode of presentation of the borderline cardinalities leads to higher over-specification rates and to higher percentages of prenominal positions than the unordered one. The paper provides further evidence for the consistency of small, borderline cardinalities, and color in people’s choices to minimally specify or over-specify given objects in referential communication.
That was just your life: rethinking dementia for advance directives
Authors: Vulliermet Franlu , Kenis Daan
Published: July 2024

Over the past decades, literature in dementia ethics has extensively looked at moral questions revolving around the care of older people living with dementia. Particularly prevalent are autonomy-related concerns regarding topics such as advance directives. In this paper, we argue that these discussions are crucially premised on how dementia is understood and represented. Despite the multiplicity of dementia presentations in people, the dominant discourse predominantly frames dementia as ‘monstrous,’ an ‘enemy,’ a destructive experience in need of eradication. We contend that such a monolithic approach, from a moral standpoint, is problematic in several respects. Indeed, framing heavily influences the way dementia is understood and experienced, leading to stigmatization, bias, and distress. Not only does it influence decisions and discussions on advance directives, but we argue that this flawed understanding of dementia is rooted in and contributes to epistemic harm. In the first section, we introduce the ethics of advance directives. More specifically, we introduce the view developed by Dworkin who has largely influenced the debate by making the case for advance directives by grounding them in the principles of autonomy and beneficence. In the second section, we show how dementia is still mostly framed monolithically as a ‘destructive experience.’ We then show that this framing is problematic because it oversteps the different pathologies dementia implies, which leads to an inaccurate representation of the condition. In the third section, we present possible alternative framings: dementia as normal aging, a person-centered care framework, and an embodied view. In the fourth section, drawing on recent developments in the epistemic injustice literature, we explore how maintaining and utilizing flawed understandings of dementia may lead to distinct moral-epistemic harms for those living with dementia and inform ongoing discussions on advance directives. Finally, in the concluding section, we return to the case of advance directives and what the implications of rethinking dementia are.
Transforming Prosthodontics and oral implantology using robotics and artificial intelligence
Authors: Karnik Atharva P. , Chhajer Harsita , Venkatesh Swapna B.
Published: July 2024

The current review focuses on how artificial intelligence (AI) and robotics can be applied to the field of Prosthodontics and oral implantology. The classification and methodologies of AI and application of AI and robotics in various aspects of Prosthodontics is summarized. The role of AI has potentially expanded in dentistry. It plays a vital role in data management, diagnosis, and treatment planning and administrative tasks. It has widespread applications in Prosthodontics owing to its immense diagnostic capability and possible therapeutic application. AI and robotics are next-generation technologies that are opening new avenues of growth and exploration for Prosthodontics. The current surge in digital human-centered automation has greatly benefited the dental field, as it transforms towards a new robotic, machine learning, and artificial intelligence era. The application of robotics and AI in the dental field aims to improve dependability, accuracy, precision, and efficiency by enabling the widespread adoption of cutting-edge dental technologies in future. Hence, the objective of the current review was to represent literature relevant to the applications of robotics and AI and in the context of diagnosis and clinical decision-making and predict successful treatment in Prosthodontics and oral implantology.
Slow light through Brillouin scattering in continuum quantum optomechanics
Authors: Zoubi Hashem , Hammerer Klemens
Published: July 2024

This study investigates the possibility of achieving a slow signal field at the level of single photons inside nanofibers by exploiting stimulated Brillouin scattering, which involves a strong pump field and the vibrational modes of the waveguide. The slow signal is significantly amplified for a pump field, with a frequency higher than that of the signal and attenuated for a lower pump frequency. We introduce a configuration for obtaining a propagating slow signal without gain or loss and with a relatively wide bandwidth. This process involves two strong pump fields with frequencies both higher and lower than that of the signal where the effects of signal amplification and attenuation compensate each other. We account for thermal fluctuations due to the scattering of thermal phonons and identify conditions under which thermal contributions to the signal field are negligible. The slowing of light through Brillouin optomechanics may serve as a vital tool for optical quantum information processing and quantum communications within nanophotonic structures.
Social-based learning and leadership in school: conflict management training for holistic, relational conflict resolution
Authors: Vinokur Eli , Yomtovian Avinoam , Marom Marva Shalev , Itzchakov Guy , Baron Liat
Published: July 2024

Navigating conflicts is crucial for promoting positive relationships between pupils, teachers, and parents. The objective of this paper is to present Social-Based Learning and Leadership (SBL), an innovative approach to group dynamics and conflict resolution within the school setting, aiming to foster meaningful relationships and personal and social growth. The methods of SBL focus on group evolution by navigating conflicts rooted in higher needs while balancing the interplay of separation and connection. It proactively embeds prosocial values and conduct into the school culture, with teachers prioritizing the wellbeing of others, fostering shared problem-solving, and positive feedback amid conflicts. Teachers acquire tools to transform the classroom into a “social laboratory” and construct meaningful partnerships with parents. Practical conflict management within the SBL framework involves dynamic group discussions, shifting from other blaming to accountability, and reflective group introspection. Experiential learning through crafted case studies and role-plays enhances students' conflict management skills by fostering perspective-taking and inclusiveness. We conducted a qualitative case study in an SBL training in a school from 2020 to 2023. These conflict management processes allow the school community to reimagine conflict as an invaluable educational opportunity, equipping pupils with essential soft skills for navigating the challenges of the 21st century.