bluuebunny/crossref_metadata_2025 huggingface dataset:
Simultaneous Temperature Measurements and Aerosol Collection During Vaping for the Analysis of Δ9-Tetrahydrocannabinol and Vitamin E Acetate Mixtures in Ceramic Coil Style Cartridges
Authors: Lynch John , Lorenz Lisa , Brueggemeyer Jana L. , Lanzarotta Adam , Falconer Travis M. , Wilson Robert A.
Published: August 2021

Incidence of e-cigarette, or vaping, product use-associated lung injury (EVALI) has been linked to the vaping of tetrahydrocannabinol (THC) products to which vitamin E acetate (VEA) has been added. In this work we vaped THC/VEA mixtures at elevated power levels using a variety of ceramic coil vaping cartridges and a commercially available vaping device, while simultaneously measuring temperature and collecting the vaporized condensate. The collected vapor condensate was analyzed for evidence of VEA decomposition by GC/MS, GC/FT-IR/MS, and LC-APCI-HRMS/MS. Mean temperature maxima for all examined cartridges at the selected power exceeded 430°C, with a range of 375–569°C, well beyond that required for thermal decomposition of VEA. The percent recovery of VEA and Δ9-THC from the vaporized mixture in six cartridges ranged from 71.5 to 101% and from 56.4 to 88.0%, respectively. Analysis of the condensed vaporized material identified VEA decomposition products duroquinone (DQ), 1-pristene, and durohydroquinone monoacetate (DHQMA); a compound consistent with 4-acetoxy-2,3,5-trimethyl-6-methylene-2,4-cyclohexadienone (ATMMC) was also detected. The concentration of DQ produced from vaporization of the THC/VEA mixture in one cartridge was found to be 4.16 ± 0.07 μg per mg of vapor condensate.
Case Report: Crown Resorption in a Patient With Junctional Epidermolysis Bullosa and Amelogenesis Imperfecta With LAMB3 Gene Mutations
Authors: Urzúa Blanca , Krämer Susanne , Morales-Bozo Irene , Camacho Claudia , Yubero María Joao , Palisson Francis , Fuentes Ignacia , Ortega-Pinto Ana
Published: August 2021

Background: Epidermolysis bullosa (EB) corresponds to a series of conditions characterized by extreme fragility of the skin and/or mucous membranes. Of the four main types of EB, junctional EB (JEB) is the most associated with alterations in the teeth. The purposes of this study were to determine the clinical, histopathological, and ultrastructural characteristics of teeth with amelogenesis imperfecta (AI) in a patient with JEB, and compare them with control teeth, and correlate the findings with the mutations present in the patient.

Case Report: The study was conducted on a 10-year-old patient with JEB carrier of two recessive mutations in the LAMB3 gene and absence of the laminin-332 protein (LM-332), determined by immunofluorescence on a skin biopsy. The patient presents hypoplastic AI with very thin and yellow-brown colored enamel. Extraction of two permanent molars was performed due to pain and soft tissue covering the crown, resembling pulp polyp or hyperplastic gingiva. Light and scanning electron microscopy (SEM) revealed very thin enamel varying from complete absence to 60 μm, absence of normal prismatic structure, and presence of a cross-banding with a laminated appearance. The histopathological study revealed granulation tissue causing external crown resorption.

Conclusion: Although coronary resorption has been reported in patients with syndromic and non-syndromic AI, this is the first clinicopathological report of coronary resorption in partially erupted teeth in patients with JEB with mutations in the LAMB3 gene and hypoplastic AI. In patients with this condition, the presence of partially erupted teeth with soft tissue covering part of the crown, without a periodontal pocket, and with a radiographic image of partial coronal radiolucency should lead to suspicion of external coronary resorption.
Differential Lipidomic Characteristics of Children Born to Women with Polycystic Ovary Syndrome
Authors: Zhang Zhirong , Liu Yue , Lv Jiali , Zhang Di , Hu Kuona , Li Jingyu , Ma Jinlong , Cui Linlin , Zhao Han
Published: August 2021

Objective

To describe the lipidomic characteristics of offspring born to polycystic ovary syndrome (PCOS) women (PCOS-off) and assess the associations between differential lipids and clinical phenotypes.

Methods

Ultra performance liquid chromatography and mass spectrometry were performed on plasma samples from 70 PCOS-off and 71 healthy controls. The associations of differential metabolites with clinical phenotypes were examined by multiple linear regression.

Results

Forty-four metabolites were significantly altered in PCOS-off, including 8 increased and 36 decreased. After stratification according to sex, 44 metabolites (13 increased and 31 decreased) were expressed differently in girls born to PCOS women (PCOS-g), most of which were glycerolipids. Furthermore, 46 metabolites (9 increased and 35 decreased) were expressed differently in boys born to PCOS women (PCOS-b), most of which were glycerophospholipids. Significant associations of metabolites with weight Z-score and high density lipoprotein cholesterol were found in PCOS-off. Triglycerides, low density lipoprotein cholesterol, and thyroid-stimulating hormone were separately correlated with some lipids in PCOS-g and PCOS-b.

Conclusions

PCOS-off showed specific lipid profile alterations. The abnormal level of glycerophospholipids and sphingomyelin indicated the risk of glucose metabolism and cardiovascular diseases in PCOS-off. Some lipids, such as phosphatidylcholines, lysophosphatidylcholine and sphingomyelin, may be the potential markers. The results broadened our understanding of PCOS-offs’ cardiometabolic status and emphasized more specific and detailed monitoring and management in this population.
Immunological Manifestations of Hepatitis E-Associated Acute and Chronic Liver Failure and Its Regulatory Mechanisms
Authors: Wu Jian , Ling Bai , Guo Naizhou , Zhai Guanghua , Li Meifen , Guo Yurong
Published: August 2021

Hepatitis E virus (HEV) is a common cause of viral hepatitis in developing countries, most commonly transmitted through the fecal-oral route. The virus is mainly of genotypes (GT) 1 and GT2 genotypes, and patients usually show symptoms of acute hepatitis. Due to the rising trend of HEV serological prevalence in global population, HEV has become an important public health problem in developed countries. Severe hepatitis caused by HEV includes acute and chronic liver failure (ACLF). ACLF frequently occurs in developed countries and is caused by overlapping chronic liver diseases of HEV with genotypes GT3 and GT4. Because the onset of hepatitis E is closely associated with immunity, it is critical to understand the immunological mechanism of hepatitis E associated with acute and chronic liver failure (HEV-ACLF). This review discusses the immunological manifestations and mechanisms of HEV-ACLF, intrahepatic immune microenvironment and treatment, and raises outstanding questions about the immunological mechanism and treatment of the disease.
Development of a Novel Restrictive Medium for Monascus Enrichment From Hongqu Based on the Synergistic Stress of Lactic Acid and Ethanol
Authors: Zhou Kangxi , Wu Li , Chen Guimei , Liu Zhibin , Zhao Xinze , Zhang Chen , Lv Xucong , Zhang Wen , Rao Pingfan , Ni Li
Published: August 2021

Graphical Abstract

The restrictive medium containing lactic acid and ethanol enabled the enrichment of Monascus sp.
Pearl Oyster Bacterial Community Structure Is Governed by Location and Tissue-Type, but Vibrio Species Are Shared Among Oyster Tissues
Authors: King William L. , Kaestli Mirjam , Siboni Nachshon , Padovan Anna , Christian Keith , Mills David , Seymour Justin , Gibb Karen
Published: August 2021

Diseases of bivalves of aquacultural importance, including the valuable Australian silver-lipped pearl oyster (Pinctada maxima), have been increasing in frequency and severity. The bivalve microbiome is linked to health and disease dynamics, particularly in oysters, with putative pathogens within the Vibrio genus commonly implicated in oyster diseases. Previous studies have been biased toward the Pacific oyster because of its global dominance in oyster aquaculture, while much less is known about the microbiome of P. maxima. We sought to address this knowledge gap by characterizing the P. maxima bacterial community, and we hypothesized that bacterial community composition, and specifically the occurrence of Vibrio, will vary according to the sampled microenvironment. We also predicted that the inside shell swab bacterial composition could represent a source of microbial spillover biofilm into the solid pearl oyster tissues, thus providing a useful predictive sampling environment. We found that there was significant heterogeneity in bacterial composition between different pearl oyster tissues, which is consistent with patterns reported in other bivalve species and supports the hypothesis that each tissue type represents a unique microenvironment for bacterial colonization. We suggest that, based on the strong effect of tissue-type on the pearl oyster bacterial community, future studies should apply caution when attempting to compare microbial patterns from different locations, and when searching for disease agents. The lack of association with water at each farm also supported the unique nature of the microbial communities in oyster tissues. In contrast to the whole bacterial community, there was no significant difference in the Vibrio community among tissue types nor location. These results suggest that Vibrio species are shared among different pearl oyster tissues. In particular, the similarity between the haemolymph, inside shell and solid tissues, suggests that the haemolymph and inside shell environment is a source of microbial spillover into the oyster tissues, and a potentially useful tool for non-destructive routine disease testing and early warning surveillance. These data provide important foundational information for future studies identifying the factors that drive microbial assembly in a valuable aquaculture species.
α3β4∗ Nicotinic Acetylcholine Receptors Strongly Modulate the Excitability of VIP Neurons in the Mouse Inferior Colliculus
Authors: Rivera-Perez Luis M. , Kwapiszewski Julia T. , Roberts Michael T.
Published: August 2021

The inferior colliculus (IC), the midbrain hub of the central auditory system, receives extensive cholinergic input from the pontomesencephalic tegmentum. Activation of nicotinic acetylcholine receptors (nAChRs) in the IC can alter acoustic processing and enhance auditory task performance. However, how nAChRs affect the excitability of specific classes of IC neurons remains unknown. Recently, we identified vasoactive intestinal peptide (VIP) neurons as a distinct class of glutamatergic principal neurons in the IC. Here, in experiments using male and female mice, we show that cholinergic terminals are routinely located adjacent to the somas and dendrites of VIP neurons. Using whole-cell electrophysiology in brain slices, we found that acetylcholine drives surprisingly strong and long-lasting excitation and inward currents in VIP neurons. This excitation was unaffected by the muscarinic receptor antagonist atropine. Application of nAChR antagonists revealed that acetylcholine excites VIP neurons mainly via activation of α3β4∗nAChRs, a nAChR subtype that is rare in the brain. Furthermore, we show that acetylcholine excites VIP neurons directly and does not require intermediate activation of presynaptic inputs that might express nAChRs. Lastly, we found that low frequency trains of acetylcholine puffs elicited temporal summation in VIP neurons, suggesting thatin vivo-like patterns of cholinergic input can reshape activity for prolonged periods. These results reveal the first cellular mechanisms of nAChR regulation in the IC, identify a functional role for α3β4∗nAChRs in the auditory system, and suggest that cholinergic input can potently influence auditory processing by increasing excitability in VIP neurons and their postsynaptic targets.
Miro1 Impairment in a Parkinson’s At-Risk Cohort
Authors: Nguyen David , Bharat Vinita , Conradson Devon M. , Nandakishore Pawan , Wang Xinnan
Published: August 2021

There is a lack of reliable molecular markers for Parkinson’s disease (PD) patients and at-risk individuals. The detection of the pre-symptomatic population of PD will empower more effective clinical intervention to delay or prevent disease onset. We have previously found that the mitochondrial protein Miro1 is resistant to mitochondrial depolarization-induced degradation in fibroblasts from a large number of PD patients and several at-risk individuals. Therefore, Miro1 has the potential to molecularly label PD populations. In order to determine whether Miro1 could serve as a molecular marker for the risk of PD, here we examine the Miro1 response to mitochondrial depolarization by biochemical approaches in induced pluripotent stem cells from a cohort of at-risk individuals. Our results show that the Miro1 phenotype is significantly associated with PD risk. We propose that Miro1 is a promising molecular marker for detecting both PD and at-risk populations. Tracking this Miro1 marker could aid in diagnosis and Miro1-based drug discoveries.
Novel Trocars and Suspension System Application in Gasless Transoral Endoscopic Thyroidectomy Vestibular Approach Oral Endoscopic Surgery
Authors: Fang Jing , Liu Jianjun , Zheng Xucai , Wang Shengying
Published: August 2021

In the current study, we reported our initial experience of gasless transoral endoscopic thyroidectomy vestibular approach (TOETVA) by novel trocars and a suspension system. Between February 2019 to September 2020, thyroid cancer patients with indicated central lymph node metastasis by imaging examination who had received gasless TOETVA by our designed trocars and suspension system in The First Affiliated Hospital of University of Science and Technology of China were reviewed. A total of 95 thyroid cancer patients that received gasless TOETVA were included in this analysis. Of note, 73 cases underwent one-sided lobectomy and the remaining 22 cases underwent total thyroidectomy. All thyroid cancer patients underwent central lymph node dissection (CND). The average total examined lymph nodes number was 8.55 ± 5.67 per individual. No serious complications occurred during or after the operation besides one patient who had a short-term recurrent laryngeal nerve (RLN) deficit and one patient who had delayed postoperative bleeding. In conclusion, the use of novel trocars and a suspension system can effectively improve the safety and efficacy of TOETVA.
Plant Stress Granules: Trends and Beyond
Authors: Maruri-López Israel , Figueroa Nicolás E. , Hernández-Sánchez Itzell E. , Chodasiewicz Monika
Published: August 2021

Stress granules (SGs) are dynamic membrane-less condensates transiently assembled through liquid–liquid phase separation (LLPS) in response to stress. SGs display a biphasic architecture constituted of core and shell phases. The core is a conserved SG fraction fundamental for its assembly and consists primarily of proteins with intrinsically disordered regions and RNA-binding domains, along with translational-related proteins. The shell fraction contains specific SG components that differ among species, cell type, and developmental stage and might include metabolic enzymes, receptors, transcription factors, untranslated mRNAs, and small molecules. SGs assembly positively correlates with stalled translation associated with stress responses playing a pivotal role during the adaptive cellular response, post-stress recovery, signaling, and metabolic rewire. After stress, SG disassembly releases mRNA and proteins to the cytoplasm to reactivate translation and reassume cell growth and development. However, under severe stress conditions or aberrant cellular behavior, SG dynamics are severely disturbed, affecting cellular homeostasis and leading to cell death in the most critical cases. The majority of research on SGs has focused on yeast and mammals as model organism. Nevertheless, the study of plant SGs has attracted attention in the last few years. Genetics studies and adapted techniques from other non-plant models, such as affinity capture coupled with multi-omics analyses, have enriched our understanding of SG composition in plants. Despite these efforts, the investigation of plant SGs is still an emerging field in plant biology research. In this review, we compile and discuss the accumulated progress of plant SGs regarding their composition, organization, dynamics, regulation, and their relation to other cytoplasmic foci. Lastly, we will explore the possible connections among the most exciting findings of SGs from mammalian, yeast, and plants, which might help provide a complete view of the biology of plant SGs in the future.
Cognitive and Linguistic Skills Associated With Cross-Linguistic Transfer in the Production of Oral Narratives in English as a Foreign Language by Arabic- and Hebrew-Speaking Children: Finding Common Denominators
Authors: Russak Susie , Zaretsky Elena
Published: August 2021

Many studies have examined literacy and related skills among learners of English as a foreign language (EFL), but little attention has been given to the role of oral language within a cross-linguistic framework despite the fact that English is the most widely spoken additional language today. Oral narratives rely on lexical, morphosyntactic, and conceptual knowledge. An in-depth examination of this modality can shed light on specific associations between cognitive and linguistic L1 and EFL skills and suggest possible mediating variables that assist multilingual speakers in producing complete oral narratives in EFL. The present study examined L1 and EFL contributors to EFL oral narratives produced by native Arabic (n= 85) and Hebrew (n= 86) speaking sixth graders seeking to identify cross-linguistic influences. We assessed general cognitive skills, phonological memory (PM), lexical, morphosyntactic knowledge, and reading comprehension in L1 (Hebrew speakers), Modern Standard Arabic (MSA, L2), L3 Hebrew (for Arabic speakers) and EFL. The “Cookie Theft” task assessed EFL elicited narratives using modified narrative analysis scales to account for microstructure (lexical and morphosyntactic complexity) and macrostructure (understanding story elements), generating a Total Narrative score. Our results yielded different patterns of underlying psycholinguistic profiles, and cross and within language associations for each group. Strong interactions between L1, L2/L3, and EFL morphological awareness and reading comprehension suggested cross-linguistic transfer. Regression analysis identified the most influential skills supporting EFL narratives for each linguistic group: English reading comprehension (ERC) was essential for Hebrew speakers and English morphological awareness (EMA) for Arabic ones. These results suggested different allocations of cognitive and linguistic resources in EFL narratives. The results also allowed to identify a common mediating skill for both groups. Findings are discussed within the theoretical framework of theInterdependence Hypothesis, theLinguistic Proximity Model, as well as accounts of direct and indirect transfer, which illuminate the impact of typological distance, general language proficiency and components of linguistic knowledge on cross-linguistic transfer in EFL oral language production.
The Role of Acceptance in Eating Behaviors—Spanish Validation of Food Craving Acceptance and Action Questionnaire (FAAQ-S)
Authors: Manchón Javier , Quiles Maria , López-Roig Sofía
Published: August 2021

Background: The Food Craving Acceptance and Action Questionnaire (FAAQ) was developed to measure food craving acceptance, but has not yet been adapted to Spanish. The aim of this study was to validate the FAAQ to the Spanish population and to analyze its psychometric properties.

Method: Two studies were conducted. In the first study, the sample consisted of 224 undergraduate students who participated in the comprehension of the Spanish version and the Confirmatory Factor Analysis (CFA). The second sample consisted of 378 participants from a community sample who completed the refined version of the FAAQ and similar and dissimilar measures.

Results: Study (1) The CFA was conducted, showing an inadequate fit of the model (CFI = 0.74, RMSEA = 0.18). Therefore, FAAQ was refined and it was administered to the community sample. Study (2) After an exploratory factor analysis, two factors were obtained as in the original FAAQ, Acceptance (30.92% variance explained) and Willingness (36.05%). The internal consistency was adequate for both subscales (ω = 0.88 and ω = 0.87, respectively). Correlation between the factors was r = 0.07, which provides evidence that Acceptance and Willingness are different constructs. Correlations of Acceptance with similar variables (r between −0.30 and −0.52) were stronger than the dissimilar measures (r between −0.26 and 0.24). This did not occur for the Willingness subscale, since correlations were low in all cases (r between −0.22 and 0.25).

Conclusions: Spanish version of the FAAQ showed evidence of its reliability and validity, and may be a measure to provide a better understanding of how acceptance of thoughts and emotions concerning food and willingness impact eating management behaviors.
California's COVID-19 Virtual Training Academy: Rapid Scale-Up of a Statewide Contact Tracing and Case Investigation Workforce Training Program
Authors: Brickley Debbie B. , Forster Maeve , Alonis Amelia , Antonyan Elizabeth , Chen Lisa , DiGiammarino Alicia , Dorian Alina , Dunn Caitlin , Gandelman Alice , Grasso Mike , Kiureghian Alice , Maher Andrew D. , Malan Hannah , Mejia Patricia , Peare Anna , Prelip Michael , Shafir Shira , White Karen , Willard-Grace Rachel , Reid Michael
Published: August 2021

Case investigation (CI) and contact tracing (CT) are key to containing the COVID-19 pandemic. Widespread community transmission necessitates a large, diverse workforce with specialized knowledge and skills. The University of California, San Francisco and Los Angeles partnered with the California Department of Public Health to rapidly mobilize and train a CI/CT workforce. In April through August 2020, a team of public health practitioners and health educators constructed a training program to enable learners from diverse backgrounds to quickly acquire the competencies necessary to function effectively as CIs and CTs. Between April 27 and May 5, the team undertook a curriculum design sprint by performing a needs assessment, determining relevant goals and objectives, and developing content. The initial four-day curriculum consisted of 13 hours of synchronous live web meetings and 7 hours of asynchronous, self-directed study. Educational content emphasized the principles of COVID-19 exposure, infectious period, isolation and quarantine guidelines and the importance of prevention and control interventions. A priority was equipping learners with skills in rapport building and health coaching through facilitated web-based small group skill development sessions. The training was piloted among 31 learners and subsequently expanded to an average weekly audience of 520 persons statewide starting May 7, reaching 7,499 unique enrollees by August 31. Capacity to scale and sustain the training program was afforded by the UCLA Extension Canvas learning management system. Repeated iteration of content and format was undertaken based on feedback from learners, facilitators, and public health and community-based partners. It is feasible to rapidly train and deploy a large workforce to perform CI and CT. Interactive skills-based training with opportunity for practice and feedback are essential to develop independent, high-performing CIs and CTs. Rigorous evaluation will continue to monitor quality measures to improve the training experience and outcomes.
Curiosity-Based Interventions Increase Everyday Functioning Score But Not Serum BDNF Levels in a Cohort of Healthy Older Adults
Authors: Grossberg Allison N. , Bettcher Brianne M. , Gorgens Kim A. , Ledreux Aurélie
Published: August 2021

An enriched environment is effective in stimulating learning and memory in animal models as well as in humans. Environmental enrichment increases brain-derived neurotrophic factor (BDNF) in aged rats and reduces levels of Alzheimer-related proteins in the blood, including amyloid-β (Aβ) peptides and misfolded toxic forms of tau. To address whether stimulation of curiosity, which is a form of enrichment, may provide a buffer against Alzheimer’s disease (AD), we measured levels of biomarkers associated with AD at baseline and after a 6-week intervention in older adults (>65 years of age) randomized to one of three different intervention conditions. Specifically, in this pilot study, we tested the effectiveness of a traditional, structured learning environment compared to a self-motivated learning environment designed to stimulate curiosity. There were no significant differences from baseline to post-intervention in any of the groups for Aβ42/Aβ40 ratio or t-tau (total-tau) plasma levels. Serum BDNF levels decreased significantly in the control group. Interestingly, individuals who had the lowest serum BDNF levels at baseline experienced significantly higher increases in BDNF over the course of the 6-week intervention compared to individuals with higher serum BDNF levels at baseline. As expected, older individuals had lower MoCA scores. Years of education correlated negatively with Aβ levels, suggesting a protective effect of education on levels of this toxic protein. ECog scores were negatively correlated with BDNF levels, suggesting that better performance on the ECog questionnaire was associated with higher BDNF levels. Collectively, these findings did not suggest that a 6-week cognitive training intervention focused on curiosity resulted in significant alterations in blood biomarkers but showed interesting correlations between cognitive scores and BDNF levels, further supporting the role of this trophic factor in brain health in older adults.
Unilateral Maximal Isometric Hex Bar Pull Test: Within-Session Reliability and Lower Body Force Production in Male and Female Freeski Athletes
Authors: McPhail Jonathan , Gonçalves Basílio A. M. , Spörri Jörg , Linnamo Vesa
Published: August 2021

The aim of the study was to (1) assess the within-session reliability of a unilateral isometric hex bar pull (UIHBP) maximal voluntary contraction (MVC) test and, (2) determine unilateral isometric absolute peak force (PFabs) and relative peak force (PF) values in freeski athletes. Twenty-one male and eight female academy to national team freeskiers performed the novel UIHBP MVC task on a force plate and PFabs and relative PF were assessed (1000 Hz). Within-session measures of PFabs offered high reliability on left and right limbs for males (ICC = 0.91–0.94, CV = 2.6–2.2%) and females (ICC = 0.94–0.94, CV = 1.4–1.6%), while relative PF measures showed good to high reliability in both left and right limbs for males (ICC = 0.8–0.84, CV = 2.6–2.2%) and females (ICC = 0.92–0.90, CV = 1.4–1.7%). We observed significantly lower PFabs (p < 0.001) and relative PF (p < 0.001) in females compared to males. No statistical difference was found between left and right limbs in males and females in PFabs (p = 0.98) and relative PF measures (p = 0.93). The UIHBP MVC test appears to be a reliable method for assessing PFabs and relative PF in male and female freeski athletes.
Melatonin Moderates the Triangle of Chronic Pain, Sleep Architecture and Immunometabolic Traffic
Authors: Chaudhry Shafqat R. , Stadlbauer Andreas , Buchfelder Michael , Kinfe Thomas M.
Published: August 2021

Preclinical as well as human studies indicate that melatonin is essential for a physiological sleep state, promotes analgesia and is involved in immunometabolic signaling by regulating neuroinflammatory pathways. Experimental and clinical neuromodulation studies for chronic pain treatment suggest that neurostimulation therapies such as spinal cord stimulation, vagus nerve stimulation and dorsal root ganglion stimulation have an impact on circulating inflammatory mediators in blood, cerebrospinal fluid and saliva. Herein, we provide an overview of current literature relevant for the shared pathways of sleep, pain and immunometabolism and elaborate the impact of melatonin on the crossroad of sleep, chronic pain and immunometabolism. Furthermore, we discuss the potential of melatonin as an adjunct to neurostimulation therapies. In this narrative review, we addressed these questions using the following search terms: melatonin, sleep, immunometabolism, obesity, chronic pain, neuromodulation, neurostimulation, neuroinflammation, molecular inflammatory phenotyping. So far, the majority of the published literature is derived from experimental studies and studies specifically assessing these relationships in context to neurostimulation are sparse. Thus, the adjunct potential of melatonin in clinical neurostimulation has not been evaluated under the umbrella of randomized-controlled trials and deserves increased attention as melatonin interacts and shares pathways relevant for noninvasive and invasive neurostimulation therapies.
When Blockchain Meets the AEC Industry: Present Status, Benefits, Challenges, and Future Research Opportunities
Authors: Cheng Mengyuan , Liu Guoliang , Xu Yongshun , Chi Ming
Published: August 2021

Blockchain is regarded as a potential technology for transforming the architecture, engineering, and construction (AEC) industry, and the number of related publications is increasing rapidly. However, a systematic review of blockchain applications in the AEC industry is lacking. The objective of this study was to review the current status of blockchain applications via a bibliometric analysis combined with a systematic literature review. According to related articles collected from databases, the present status of blockchain was analysed with regard to the distribution of articles over publication years, journals, institutions, countries, cooperation networks between authors, keyword co-occurrence networks, and research methodologies. The results indicated that research on blockchain applications is still relatively new and fragmented with regard to several topics. Five areas of benefit were identified: (i) supply chain management, (ii) contract management, (iii) information management, (iv) stakeholder management, and (v) integration management. On the basis of the technology–organisation–environment framework, nine types of challenges were identified. Future research opportunities were proposed according to the research findings. This study contributes to the current body of knowledge and provides guidelines for further research on blockchain applications in the AEC industry.
SOX2 and Bcl-2 as a Novel Prognostic Value in Hepatocellular Carcinoma Progression
Authors: Hosseini-khah Zahra , Babaei Mohammad Reza , Tehrani Mohsen , Cucchiarini Magali , Madry Henning , Ajami Abolghasem , Rakhshani Nasser , Rafiei Alireza , Nikbin Behrooz
Published: August 2021

Sex-determining region Y-box 2 (SOX2) is a stem cell transcription factor and a major regulator of self-renewal and pluripotency of cancer stem cells (CSCs). In many types of cancer, SOX2 is dysregulated due to overexpression associated with tumor progression and low survival rate. Many HCC cases encounter recurrence and metastasis which might be due to CSCs and also apoptosis. Since little is known about the expression pattern of SOX2 and apoptotic genes in HCC, we aimed to determine the prognostic significance of SOX2, Bax, and Bcl-2 in clinicopathological features, tumor progression, and survival rate of the HCC patients. The expression of SOX2, Bax, and Bcl-2 were evaluated using qRT-PCR in 53 formalin-fixed, paraffin-embedded tissues (FFPE) of patients and 44 controls. Correlation of these genes was analyzed with clinicopathological features and tumor progression. The correlationship between SOX2 expression and ALBI grade as prognostic indicators were calculated. Survival rates were determined by Kaplan–Meier survival curves. SOX2 and Bcl-2 were remarkably overexpressed in HCC patients compared to controls (p = 0.04 and p = 0.003, respectively). A significant association was found for both SOX2 and Bcl-2 overexpression with TNM staging (p = 0.02, p = 0.04) and tumor grading (p = 0.01, p = 0.003), respectively. A significant correlation was observed: patients with SOX2 overexpression had a lower 5-year overall survival rate (p = 0.04); however, there was no significant association between Bcl-2 and survival (p = 0.5). Collectively, overexpression of SOX2 and Bcl-2, alone or combined, may be a potential marker to evaluate prognosis and response to HCC treatment.
Bispectral Analysis of Heart Rate Variability to Characterize and Help Diagnose Pediatric Sleep Apnea
Authors: Martín-Montero Adrián , Gutiérrez-Tobal Gonzalo C. , Gozal David , Barroso-García Verónica , Álvarez Daniel , del Campo Félix , Kheirandish-Gozal Leila , Hornero Roberto
Published: August 2021

Pediatric obstructive sleep apnea (OSA) is a breathing disorder that alters heart rate variability (HRV) dynamics during sleep. HRV in children is commonly assessed through conventional spectral analysis. However, bispectral analysis provides both linearity and stationarity information and has not been applied to the assessment of HRV in pediatric OSA. Here, this work aimed to assess HRV using bispectral analysis in children with OSA for signal characterization and diagnostic purposes in two large pediatric databases (0–13 years). The first database (training set) was composed of 981 overnight ECG recordings obtained during polysomnography. The second database (test set) was a subset of the Childhood Adenotonsillectomy Trial database (757 children). We characterized three bispectral regions based on the classic HRV frequency ranges (very low frequency: 0–0.04 Hz; low frequency: 0.04–0.15 Hz; and high frequency: 0.15–0.40 Hz), as well as three OSA-specific frequency ranges obtained in recent studies (BW1: 0.001–0.005 Hz; BW2: 0.028–0.074 Hz; BWRes: a subject-adaptive respiratory region). In each region, up to 14 bispectral features were computed. The fast correlation-based filter was applied to the features obtained from the classic and OSA-specific regions, showing complementary information regarding OSA alterations in HRV. This information was then used to train multi-layer perceptron (MLP) neural networks aimed at automatically detecting pediatric OSA using three clinically defined severity classifiers. Both classic and OSA-specific MLP models showed high and similar accuracy (Acc) and areas under the receiver operating characteristic curve (AUCs) for moderate (classic regions: Acc = 81.0%, AUC = 0.774; OSA-specific regions: Acc = 81.0%, AUC = 0.791) and severe (classic regions: Acc = 91.7%, AUC = 0.847; OSA-specific regions: Acc = 89.3%, AUC = 0.841) OSA levels. Thus, the current findings highlight the usefulness of bispectral analysis on HRV to characterize and diagnose pediatric OSA.
Fast Compression of MCMC Output
Authors: Chopin Nicolas , Ducrocq Gabriel
Published: August 2021

We propose cube thinning, a novel method for compressing the output of an MCMC (Markov chain Monte Carlo) algorithm when control variates are available. It allows resampling of the initial MCMC sample (according to weights derived from control variates), while imposing equality constraints on the averages of these control variates, using the cube method (an approach that originates from survey sampling). The main advantage of cube thinning is that its complexity does not depend on the size of the compressed sample. This compares favourably to previous methods, such as Stein thinning, the complexity of which is quadratic in that quantity.
Multifractal Behaviors of Stock Indices and Their Ability to Improve Forecasting in a Volatility Clustering Period
Authors: Zhang Shuwen , Fang Wen
Published: August 2021

The financial market is a complex system, which has become more complicated due to the sudden impact of the COVID-19 pandemic in 2020. As a result there may be much higher degree of uncertainty and volatility clustering in stock markets. How does this “black swan” event affect the fractal behaviors of the stock market? How to improve the forecasting accuracy after that? Here we study the multifractal behaviors of 5-min time series of CSI300 and S&P500, which represents the two stock markets of China and United States. Using the Overlapped Sliding Window-based Multifractal Detrended Fluctuation Analysis (OSW-MF-DFA) method, we found that the two markets always have multifractal characteristics, and the degree of fractal intensified during the first panic period of pandemic. Based on the long and short-term memory which are described by fractal test results, we use the Gated Recurrent Unit (GRU) neural network model to forecast these indices. We found that during the large volatility clustering period, the prediction accuracy of the time series can be significantly improved by adding the time-varying Hurst index to the GRU neural network.
Principle of Least Effort and Sentence Length in Public Speaking
Authors: Tsizhmovska Natalia L. , Martyushev Leonid M.
Published: August 2021

The analysis of sentence lengths in the inaugural speeches of US presidents and the annual speeches of UK party leaders is carried out. Transcripts of the speeches are used, rather than the oral production. It is discovered that the average sentence length in these speeches decreases linearly with time, with the slope of 0.13 ± 0.03 words/year. It is shown that among the analyzed distributions (log-normal, folded and half normal, Weibull, generalized Pareto, Rayleigh) the Weibull is the best distribution for describing sentence length. These two results can be considered a consequence of the principle of least effort. The connection of this principle with the well-known principles of maximum and minimum entropy production is discussed.
Evaluating Temporal Correlations in Time Series Using Permutation Entropy, Ordinal Probabilities and Machine Learning
Authors: Boaretto Bruno R. R. , Budzinski Roberto C. , Rossi Kalel L. , Prado Thiago L. , Lopes Sergio R. , Masoller Cristina
Published: August 2021

Time series analysis comprises a wide repertoire of methods for extracting information from data sets. Despite great advances in time series analysis, identifying and quantifying the strength of nonlinear temporal correlations remain a challenge. We have recently proposed a new method based on training a machine learning algorithm to predict the temporal correlation parameter, α, of flicker noise (FN) time series. The algorithm is trained using as input features the probabilities of ordinal patterns computed from FN time series, xαFN(t), generated with different values of α. Then, the ordinal probabilities computed from the time series of interest, x(t), are used as input features to the trained algorithm and that returns a value, αe, that contains meaningful information about the temporal correlations present in x(t). We have also shown that the difference, Ω, of the permutation entropy (PE) of the time series of interest, x(t), and the PE of a FN time series generated with α=αe, xαeFN(t), allows the identification of the underlying determinism in x(t). Here, we apply our methodology to different datasets and analyze how αe and Ω correlate with well-known quantifiers of chaos and complexity. We also discuss the limitations for identifying determinism in highly chaotic time series and in periodic time series contaminated by noise. The open source algorithm is available on Github.
Applicability of Concrete–Steel Composite Piles for Offshore Wind Foundations
Authors: Shin Yunsup , Langford Thomas , Cho Kyunghwan , Park Jongheon , Ko Junyoung
Published: August 2021

Offshore wind-turbine support structures are largely made of steel since steel monopiles have accounted for the majority of installations in the last decade. As turbines become bigger, steel structures have led to an exponential increase in material and installation costs. From this point of view, the use of concrete for future support structures has been initiated. In this study, concrete–steel composite piles have been investigated. A pre-tensioned high strength concrete pile was placed in the lower part, mainly to support the axial load, and a steel pile in the upper part to resist the lateral load. A mechanical joint was adapted to connect the two different types of piles. Static axial, dynamic axial, and lateral load tests were performed to evaluate the load-displacement response of the composite pile, verify the integrity of the mechanical joint, and investigate its potential application to offshore wind foundations. This paper focused on the load-displacement response and the connection integrity; in particular, it investigated the lateral load-displacement response by comparing it to the results of beam-spring analysis. Based on the results from the field tests, a site-specific lateral load-displacement curve was suggested, and the connection integrity was verified.
Do People Prefer Cars That People Don’t Drive? A Survey Study on Autonomous Vehicles
Authors: Meidute-Kavaliauskiene Ieva , Yıldız Bülent , Çiğdem Şemsettin , Činčikaitė Renata
Published: August 2021

Only recently, smart cities are taking shape, thanks to the rapid development of Internet of Things (IoT), cloud computing, and other similar technologies. Given the high demands placed on advanced technologies such as autonomous driving, cloud data services, and high-precision sensors, smart cities are creating an intelligent transportation environment conducive to the introduction of autonomous vehicles (AVs). In this context, the use of AVs in transportation is also considered a form of transportation innovation. As a result, AVs are considered more favorable to people interested in new technologies because they appear to be technologically superior. Their association with the most up-to-date technology can serve as a symbol for those who wish to demonstrate their interest in new technologies through their appearance. The positive image of technological innovation projected by AVs may influence their acceptance among technology enthusiasts to a significant degree. In this context, this study investigates the effects of perceived advantage, perceived risk, and perceived safety on the intention to use autonomous vehicles. For this purpose, data were collected from vehicle users living in Turkey by survey method. Secondly, factor analyses and regression analyses were performed with the data set obtained from 611 participants. As a result of the analyses, it has been determined that the perceived advantage and perceived security increase the intention to use autonomous vehicles. In contrast, the perceived risk reduces this intention to use. According to these results, recommendations were made to the companies about the level of acceptance of this technology by the users to assess their investments in autonomous vehicles better.
Lessons from the Electric Vehicle Crashworthiness Leading to Battery Fire
Authors: Victor Chombo Pius , Laoonual Yossapong , Wongwises Somchai
Published: August 2021

Electric vehicles (EVs) are currently emerging as alternative vehicles due to their high energy efficiency and low emissions during driving. However, regarding the raising concern, the safety of EVs can further be improved before they completely replace conventional vehicles. This paper focuses on reviewing the safety requirements of EVs, especially those powered by Li-ion battery, based on the mechanical abuse tests from the international standards, national standards, regulations and other laboratories standards, and safety of occupants from the regulations and safety programs. Moreover, the publicly reported real-world fire incidents of EVs based on road crashes were collected and reviewed. The objective is to highlight the gap and challenges arose between the current safety requirements and real-world fire incidents of EVs and provide the way for assisting the future research in the area of EV safety, particularly light duty passenger vehicle. The serious challenges observed include high impact speed, multi-crashes per incident, multiple barriers of different types involved in the accident, and post-crash safety (serious injury and demise) of occupants and rescue teams. While addressing these challenges, this review will aid researchers and manufacturers working in batteries, EVs, and fire safety engineering to narrow the gap and enhance the safety of future EVs in areas of battery materials, fire extinguishing, and vehicle’s body structure.
Assessment of the Total Cost of Ownership of Electric Vehicles in Poland
Authors: Ewelina Sendek-Matysiak , Grysa Krzysztof
Published: August 2021

Eliminating environmental and air pollution is one of the European Union’s priority actions in the field of transport. Poland, as a member of the European Community, is also actively involved in these activities. The flagship project, the implementation of which is expected to bring tangible effects in this respect, is the “Sustainable Transport Development Strategy until 2030”. It states that in 2030 there should be 600,000 BEVs (battery electric vehicles) on Polish roads. At present, the share of such vehicles in the automotive market in Poland is small, which is a result of a number of barriers. One of them, very important considering the preferences of Poles, is the cost of buying such a car, which is currently at least 40% higher than its counterpart with a combustion engine. Meanwhile, popularizers of electric cars believe that the cost of buying such a vehicle is offset by the subsequent costs associated with its operation. Hence, this paper determines and then compares the total purchase and operating costs of cars of the same make, same model, differing in the source of propulsion, to category M1. Cars in this category represent the largest share of the automotive market in Poland—over 75%. The main objective of the analysis conducted was to determine if a current electric passenger vehicle can be competitive with an internal combustion car in everyday use. Therefore, a relationship was developed to calculate the total cost of ownership, which takes into account all the key criteria from the point of view of private vehicle use. The utilitarian value of this research may be supported by the fact that the example of Poland and its problems concerning the issue in question may serve as a source of preliminary analysis for other countries.
Optimization of Charge Pump Based on Piecewise Modeling of Output-Voltage Ripple
Authors: Lin Yajun , Yang Jianxin , Mui Tin-Wai , Zhou Yong , Leung Ka-Nang
Published: August 2021

This work proposes a piecewise modeling of output-voltage ripple for linear charge pumps. The proposed modeling can obtain a more accurate design expression of power-conversion efficiency. The relationship between flying and output capacitance, as well as switching frequency and optimize power-conversion efficiency can be calculated. The proposed modeling is applied to three charge-pump circuits: 1-stage linear charge pump, dual-branch 1-stage linear charge pump and 4× cross-coupled charge pump. Circuit-level simulation is used to verify the accuracy of proposed modeling.