bluuebunny/crossref_metadata_2025 huggingface dataset:
Recent Achievements in the Heterogeneity of Mammalian and Human Retinal Pigment Epithelium: In Search of a Stem Cell
Authors: Rzhanova Lyubov A. , Markitantova Yuliya V. , Aleksandrova Maria A.
Published: February 2024

Retinal pigment epithelium (RPE) cells are important fundamentally for the development and function of the retina. In this regard, the study of the morphological and molecular properties of RPE cells, as well as their regenerative capabilities, is of particular importance for biomedicine. However, these studies are complicated by the fact that, despite the external morphological similarity of RPE cells, the RPE is a population of heterogeneous cells, the molecular genetic properties of which have begun to be revealed by sequencing methods only in recent years. This review carries out an analysis of the data from morphological and molecular genetic studies of the heterogeneity of RPE cells in mammals and humans, which reveals the individual differences in the subpopulations of RPE cells and the possible specificity of their functions. Particular attention is paid to discussing the properties of “stemness,” proliferation, and plasticity in the RPE, which may be useful for uncovering the mechanisms of retinal diseases associated with pathologies of the RPE and finding new ways of treating them.
In Vitro Lipid Overload Affects Cellular Proliferation, Apoptosis, and Senescence in a Time-Dependent Manner in HepG2 Hepatocytes and LX-2 Hepatic Stellate Cells
Authors: Campos-Espinosa Adriana , Guzmán Carolina , Medina-Ávila Karla Zaira , Gutierrez-Reyes Gabriela
Published: February 2024

Different cellular mechanisms influence steatotic liver disease (SLD) progression. The influence of different levels of steatogenic inputs has not been studied in hepatocytes and hepatic stellate cells (HSCs). Methods: HepG2 hepatocytes and LX-2 HSCs were cultured in mild (MS) and severe (SS) steatogenic conditions. TGF-β stimulation was also tested for HSCs in control (T) and steatogenic conditions (MS-T and SS-T). Steatosis was stained with Oil Red, and the proliferation was assayed via WST-8 reduction, apoptosis via flow cytometry, and senescence via SA-β-galactosidase activity. Results: Regarding hepatocytes, steatosis progressively increased; proliferation was lower in MS and SS; and the viability of both conditions significantly decreased at 72 h. Apoptosis increased in MS at 72 h, while it decreased in SS. Senescence increased in MS and diminished in SS. Regarding HSCs, the SS and SS-T groups showed no proliferation, and the viability was reduced in MS at 72 h and in SS and SS-T. The LX-2 cells showed increased apoptosis in SS and SS-T at 24 h, and in MS and MS-T at 72 h. Senescence decreased in MS, SS, and SS-T. Conclusions: Lipid overload induces differential effects depending on the cell type, the steatogenic input level, and the exposure time. Hepatocytes are resilient to mild steatosis but susceptible to high lipotoxicity. HSCs are sensitive to lipid overload, undergoing apoptosis and lowering senescence and proliferation. Collectively, these data may help explain the development of steatosis and fibrosis in SLD.
Cardiopulmonary Exercise Performance of Children Born Non-Extremely Preterm
Authors: Fouzas Sotirios , Nourloglou Aikaterini , Vervenioti Aggeliki , Karatza Ageliki , Anthracopoulos Michael B. , Dimitriou Gabriel
Published: February 2024

Data on exercise tolerance of children born non-extremely preterm are sparse. We aimed to explore the cardiopulmonary exercise testing (CPET) characteristics in this population. We studied 63 children (age 7–12 years) born at 290/7–366/7 weeks of gestation (34 were late preterm, 29 were preterm) and 63 age-matched, term-born controls. All performed spirometry and CPET (cycle ergometry). There were no differences in activity levels and spirometric parameters between the group of preterm-born children and controls. A peak oxygen uptake (VO2peak) of <80% was noted in 25.4% of the term-born and 49.2% of preterm-born children (p = 0.001). Term-born participants presented similar VO2peak to late-preterm children but higher than those born at <340/7 weeks of gestation (p = 0.002). Ventilatory limitation was noted in 4.8% of term and 7.9% of preterm participants, while only one preterm child presented cardiovascular limitation. Children born before 34 weeks of gestation had higher respiratory rates and smaller tidal volumes at maximum exercise, as well as lower oxygen uptake for the level of generated work. We conclude that school-age children born at 29–34 weeks of gestation may present decreased exercise performance attributed to an altered ventilatory response to exercise and impaired O2 utilization by their skeletal muscles rather than other cardiopulmonary limiting factors.
Genomic Determinants of Knee Joint Biomechanics: An Exploration into the Molecular Basis of Locomotor Function, a Narrative Review
Authors: Iacobescu Georgian-Longin , Iacobescu Loredana , Popa Mihnea Ioan Gabriel , Covache-Busuioc Razvan-Adrian , Corlatescu Antonio-Daniel , Cirstoiu Catalin
Published: February 2024

In recent years, the nexus between genetics and biomechanics has garnered significant attention, elucidating the role of genomic determinants in shaping the biomechanical attributes of human joints, specifically the knee. This review seeks to provide a comprehensive exploration of the molecular basis underlying knee joint locomotor function. Leveraging advancements in genomic sequencing, we identified specific genetic markers and polymorphisms tied to key biomechanical features of the knee, such as ligament elasticity, meniscal resilience, and cartilage health. Particular attention was devoted to collagen genes like COL1A1 and COL5A1 and their influence on ligamentous strength and injury susceptibility. We further investigated the genetic underpinnings of knee osteoarthritis onset and progression, as well as the potential for personalized rehabilitation strategies tailored to an individual’s genetic profile. We reviewed the impact of genetic factors on knee biomechanics and highlighted the importance of personalized orthopedic interventions. The results hold significant implications for injury prevention, treatment optimization, and the future of regenerative medicine, targeting not only knee joint health but joint health in general.
Nicotinamide Riboside Regulates Chemotaxis to Decrease Inflammation and Ameliorate Functional Recovery Following Spinal Cord Injury in Mice
Authors: Li Yan , Zhang Chunjia , Li Zihan , Bai Fan , Jing Yingli , Ke Han , Zhang Shuangyue , Yan Yitong , Yu Yan
Published: February 2024

Changes in intracellular nicotinamide adenine dinucleotide (NAD+) levels have been observed in various disease states. A decrease in NAD+ levels has been noted following spinal cord injury (SCI). Nicotinamide riboside (NR) serves as the precursor of NAD+. Previous research has demonstrated the anti-inflammatory and apoptosis-reducing effects of NR supplements. However, it remains unclear whether NR exerts a similar role in mice after SCI. The objective of this study was to investigate the impact of NR on these changes in a mouse model of SCI. Four groups were considered: (1) non-SCI without NR (Sham), (2) non-SCI with NR (Sham +NR), (3) SCI without NR (SCI), and (4) SCI with NR (SCI + NR). Female C57BL/6J mice aged 6–8 weeks were intraperitoneally administered with 500 mg/kg/day NR for a duration of one week. The supplementation of NR resulted in a significant elevation of NAD+ levels in the spinal cord tissue of mice after SCI. In comparison to the SCI group, NR supplementation exhibited regulatory effects on the chemotaxis/recruitment of leukocytes, leading to reduced levels of inflammatory factors such as IL-1β, TNF-α, and IL-22 in the injured area. Moreover, NR supplementation notably enhanced the survival of neurons and synapses within the injured area, ultimately resulting in improved motor functions after SCI. Therefore, our research findings demonstrated that NR supplementation had inhibitory effects on leukocyte chemotaxis, anti-inflammatory effects, and could significantly improve the immune micro-environment after SCI, thereby promoting neuronal survival and ultimately enhancing the recovery of motor functions after SCI. NR supplementation showed promise as a potential clinical treatment strategy for SCI.
Influence of Spray Angle on Particle Deposition and Thermal Shock Lifetime of Embedded Micro-Agglomerated Particle Coatings
Authors: Tang Zhongxiang , Yang Ting , Zhang Chengcheng , Wang Weize , Liu Shuainan , Liu Wei , Liu Chen
Published: February 2024

The development of gas turbine technology has led to an increase in the complexity of the geometric shape of the sprayed workpiece. Consequently, it has become more difficult to maintain the perpendicularity of the spraying angle during the spraying process, thereby impacting the structure and performance of the coating. This study uses the atmospheric plasma spraying method to simultaneously spray two types of powder for the preparation of embedded micro-agglomerated particle (EMAP) coatings. The spraying process is conducted at four different angles, ranging from 90° to 30°, in order to analyze the influence of the spray angle on the particle deposition and coating performance. The experimental results demonstrate that the relative deposition efficiency, hardness, and elastic modulus of the EMAP coatings decreased as the spray angle decreased. The porosity exhibited a reduction when the spraying angle dropped from 90° to 50°, followed by a significant rise at 30°. The greatest relative amount of second phase particles embedded in the coating appeared at a spraying angle of 90°, amounting to 10.8%. The smallest amount was found at a spraying angle of 30°, with a relative quantity of 2.2%. Furthermore, the molten droplets of the first phase matrix powder underwent extension and fragmentation along the angular direction at low angles. At an angle of 90°, the maximum average thermal shock life was 40.6 cycles, with the best stability of thermal shock life. The decrease in the spraying angle resulted in a deterioration in both the thermal shock life and its stability.
A Phase-Field Regularized Cohesion Model for Hydrogen-Assisted Cracking
Authors: Zhang Shiyi , Shen Qiang
Published: February 2024

Hydrogen-assisted cracking (HAC) usually causes premature mechanical failure of the material and results in structural damage in hydrogen environments. A phase-field regularized cohesion model (PF-CZM) was proposed to address hydrogen-assisted cracking. It incorporated the hydrogen-enhanced decohesion mechanism to decrease the critical energy release rate to address damage initiation and progression in a chemo-mechanical coupled environment. This model is based on coupled mechanical and hydrogen diffusion responses, driven by chemical potential gradients, and the introduction of hydrogen-related fracture energy degradation laws. The coupling problem is solved by an implicit time integral, in which hydrogen concentration, displacement and phase-field order parameters are the main variables. Three commonly used loading regimes (tension, shear, and three-point bending) were provided for comparing crack growth. Specifically, (i) hydrogen-dependent fracture energy degradation, (ii) mechanical–chemical coupling, and (iii) the diffusion coefficient D is influenced by both the phase field and the chemical field. By considering these factors, the PF-CZM model provided a variational framework by coupling mechanical loading with concentration diffusion for studying the complex interplay between a chemo-mechanical coupled environment and material damage, thereby enhancing our understanding of hydrogen-assisted cracking phenomena.
A User-Centered Privacy Policy Management System for Automatic Consent on Cookie Banners
Authors: Porcelli Lorenzo , Mastroianni Michele , Ficco Massimo , Palmieri Francesco
Published: February 2024

Despite growing concerns about privacy and an evolution in laws protecting users’ rights, there remains a gap between how industries manage data and how users can express their preferences. This imbalance often favors industries, forcing users to repeatedly define their privacy preferences each time they access a new website. This process contributes to the privacy paradox. We propose a user support tool named the User Privacy Preference Management System (UPPMS) that eliminates the need for users to handle intricate banners or deceptive patterns. We have set up a process to guide even a non-expert user in creating a standardized personal privacy policy, which is automatically applied to every visited website by interacting with cookie banners. The process of generating actions to apply the user’s policy leverages customized Large Language Models. Experiments demonstrate the feasibility of analyzing HTML code to understand and automatically interact with cookie banners, even implementing complex policies. Our proposal aims to address the privacy paradox related to cookie banners by reducing information overload and decision fatigue for users. It also simplifies user navigation by eliminating the need to repeatedly declare preferences in intricate cookie banners on every visited website, while protecting users from deceptive patterns.
From Molecular Biology to Novel Immunotherapies and Nanomedicine in Uveal Melanoma
Authors: Synoradzki Kamil J. , Paduszyńska Natalia , Solnik Malgorzata , Toro Mario Damiano , Bilmin Krzysztof , Bylina Elżbieta , Rutkowski Piotr , Yousef Yacoub A. , Bucolo Claudio , Zweifel Sandrine Anne , Reibaldi Michele , Fiedorowicz Michal , Czarnecka Anna M.
Published: February 2024

Molecular biology studies of uveal melanoma have resulted in the development of novel immunotherapy approaches including tebentafusp—a T cell–redirecting bispecific fusion protein. More biomarkers are currently being studied. As a result, combined immunotherapy is being developed as well as immunotherapy with bifunctional checkpoint inhibitory T cell engagers and natural killer cells. Current trials cover tumor-infiltrating lymphocytes (TIL), vaccination with IKKb-matured dendritic cells, or autologous dendritic cells loaded with autologous tumor RNA. Another potential approach to treat UM could be based on T cell receptor engineering rather than antibody modification. Immune-mobilizing monoclonal T cell receptors (TCR) against cancer, called ImmTAC TM molecules, represent such an approach. Moreover, nanomedicine, especially miRNA approaches, are promising for future trials. Finally, theranostic radiopharmaceuticals enabling diagnosis and therapy with the same molecule bring hope to this research.
Quality of Life Longitudinal Evaluation in Prostate Cancer Patients from Radiotherapy Start to 5 Years after IMRT-IGRT
Authors: Maggio Angelo , Rancati Tiziana , Gatti Marco , Cante Domenico , Avuzzi Barbara , Bianconi Cinzia , Badenchini Fabio , Farina Bruno , Ferrari Paolo , Giandini Tommaso , Girelli Giuseppe , Landoni Valeria , Magli Alessandro , Moretti Eugenia , Petrucci Edoardo , Salmoiraghi Paolo , Sanguineti Giuseppe , Villa Elisa , Waskiewicz Justyna Magdalena , Guarneri Alessia , Valdagni Riccardo , Fiorino Claudio , Cozzarini Cesare
Published: February 2024

Purpose: The purpose of this study is to study the evolution of quality of life (QoL) in the first 5 years following Intensity-modulated radiation therapy (IMRT) for prostate cancer (PCa) and to determine possible associations with clinical/treatment data. Material and methods: Patients were enrolled in a prospective multicentre observational trial in 2010-2014 and treated with conventional (74–80 Gy, 1.8–2 Gy/fr) or moderately hypofractionated IMRT (65–75.2 Gy, 2.2–2.7 Gy/fr). QoL was evaluated by means of EORTC QLQ-C30 at baseline, at radiation therapy (RT) end, and every 6 months up to 5 years after IMRT end. Fourteen QoL dimensions were investigated separately. The longitudinal evaluation of QoL was analysed by means of Analysis of variances (ANOVA) for multiple measures. Results: A total of 391 patients with complete sets of questionnaires across 5 years were available. The longitudinal analysis showed a trend toward the significant worsening of QoL at RT end for global health, physical and role functioning, fatigue, appetite loss, diarrhoea, and pain. QoL worsening was recovered within 6 months from RT end, with the only exception being physical functioning. Based on ANOVA, the most impaired time point was RT end. QoL dimension analysis at this time indicated that acute Grade ≥ 2 gastrointestinal (GI) toxicity significantly impacted global health, physical and role functioning, fatigue, appetite loss, diarrhoea, and pain. Acute Grade ≥ 2 genitourinary (GU) toxicity resulted in lower role functioning and higher pain. Prophylactic lymph-nodal irradiation (WPRT) resulted in significantly lower QoL for global health, fatigue, appetite loss, and diarrhoea; lower pain with the use of neoadjuvant/concomitant hormonal therapy; and lower fatigue with the use of an anti-androgen. Conclusions: In this prospective, longitudinal, observational study, high radiation IMRT doses delivered for PCa led to a temporary worsening of QoL, which tended to be completely resolved at six months. Such transient worsening was mostly associated with acute GI/GU toxicity, WPRT, and higher prescription doses.
Mangrove Rehabilitation and Brachyuran Crab Biodiversity in Ranong, Thailand
Authors: Ashton Elizabeth C. , Macintosh Donald J.
Published: February 2024

Mangroves rehabilitated after deforestation by commercial exploitation must be monitored to confirm that key ecosystem functions are being restored. Brachyuran crabs are conspicuous mangrove macrofauna and were selected as potential indicators of ecosystem recovery. A deforested former mangrove charcoal concession area in Ranong was rehabilitated by planting Rhizophora (1994), Bruguiera and Ceriops (1995) seedlings in single-species blocks. A second area, deforested and heavily degraded by tin mining, was rehabilitated with R. mucronata in 1985. Crabs at these sites were compared with those in a mixed-species conservation forest. Timed collections were made in 1999, 2008 and 2019 to compare crab diversity and relative abundance between sites and years. Thirty-three brachyuran crab species were recorded. Fiddler crabs (Austruca triangularis, Tubuca rosea) and the signal crab, Metaplax elegans, were most abundant, followed by sesarmid crabs (15 species). Species composition differed significantly between sites but not between the four planted tree species blocks. We propose Metaplax elegans as an indicator of ecological development in low-lying/newly formed sediments; fiddler crabs as equivalent indicators in young mangrove plantations/open forest habitats; and a diverse sesarmid community to indicate ecological functioning in older plantations/dense forests.
Effects of Nitrogen on the Bacterial Microbiome Community of Oocystis borgei, an Alga Widely Used in Marine Aquaculture
Authors: Liu Bihong , Hu Zhangxi , Li Changling , Huang Xianghu , Zhang Yulei
Published: February 2024

In order to investigate the effects of various nitrogen (ammonium) concentrations on the epibiotic bacterial community associated with Oocystis borgei, a metabarcoding sequencing method was employed. The 16S rDNA sequencing and bioinformatic analysis were conducted on cultures of O. borgei that were grown on four different nitrogen element concentrations (2.5, 10, 50, and 100 mg/L), and the differences in the epibiotic bacterial community and functions of O. borgei among different nitrogen concentrations were compared. The results showed that the chlorophyll a content of O. borgei increased with increasing nitrogen concentrations. A total of 43 operational taxonomic units (OTUs) were obtained from the four groups, which were categorized into 7 phyla, 9 classes, 18 orders, 21 families, and 26 genera. Winogradskyella was the dominant genus in the groups with 2.5 and 100 mg/L nitrogen, while Marinobacter and Winogradskyella were relatively abundant in the groups with 50 and 100 mg/L nitrogen. Functional analysis using PICRUST2 showed that the three most abundant gene functions were carbohydrate metabolism, amino acid transport and metabolism, ribosomal structure and biogenesis, and energy production and conversion. BugBase phenotypic analysis revealed that there were no significant differences in phenotypes between the groups with 2.5 and 10 mg/L nitrogen, while the group with 50 mg/L nitrogen exhibited higher abundance in aerobicity, biofilm formation, mobile genetic elements, and stress tolerance phenotypes. Most of the bacteria in this work belonged to the aerobic types. A redundancy analysis (RDA) of environmental factors demonstrated that nitrogen concentration showed a positive correlation with species changes in the groups with 10 and 50 mg/L nitrogen; chlorophyll a exhibited a positive correlation with species changes in the groups with 50 and 100 mg/L nitrogen. Nitrogen concentration significantly influenced the epibiotic bacterial community associated with O. borgei, leading to changes in dominant species and community structure. This study provides important references for understanding the functional characteristics of the epiphytic microbial community of O. borgei and the exploration of specific microorganisms.
Advantages of the Utilization of Wide-Field OCT and Wide-Field OCT Angiography in Clinical Practice
Authors: Gawęcki Maciej , Kiciński Krzysztof
Published: February 2024

Wide-field (WF) retinal imaging is becoming a standard diagnostic tool for diseases involving the peripheral retina. Technological progress elicited the advent of wide-field optical coherence tomography (WF-OCT) and WF-OCT angiography (WF-OCTA) examinations. This review presents the results of studies that analyzed the implementation of these procedures in clinical practice and refers to them as traditional and ultra-wide-field fluorescein angiography (UWF-FA). A PUBMED search was performed using the terms WF-OCT OR WF-OCTA OR UWF-FA AND the specific clinical entity, and another search for diabetic retinopathy (DR), retinal vein occlusion (RVO), Coats disease, peripheral retinal telangiectasia, peripheral retinal degeneration, lattice degeneration, and posterior vitreous detachment. The analysis only included the studies in which the analyzed field of view for the OCT or OCTA exam was larger than 55 degrees. The evaluation of the extracted studies indicates that WF imaging with OCT and OCTA provides substantial information on retinal disorders involving the peripheral retina. Vascular diseases, such as DR or RVO, can be reliably evaluated using WF-OCTA with results superior to standard-field fluorescein angiography. Nevertheless, UWF-FA provides a larger field of view and still has advantages over WF-OCTA concerning the evaluation of areas of non-perfusion and peripheral neovascularization. Detailed information on the vascular morphology of peripheral changes should be obtained via WF-OCTA and not angiographic examinations. WF-OCT can serve as a valuable tool for the detection and evaluation of vitreoretinal traction, posterior vitreous detachment, and peripheral retinal degeneration, and guide therapeutic decisions on a patient’s eligibility for surgical procedures.
Musculoskeletal and Neuropathic Pain in COVID-19
Authors: Lam Christopher M. , Sanderson Miles , Vu Dan T. , Sayed Dawood , Latif Usman , Chadwick Andrea L. , Staats Peter , York Abigail , Smith Gabriella , Velagapudi Vivek , Khan Talal W.
Published: February 2024

Chronic pain constitutes a significant disease burden globally and accounts for a substantial portion of healthcare spending. The COVID-19 pandemic contributed to an increase in this burden as patients presented with musculoskeletal or neuropathic pain after contracting COVID-19 or had their chronic pain symptoms exacerbated by the virus. This extensive literature review analyzes the epidemiology of pain pre-pandemic, the costs associated with the COVID-19 pandemic, the impact of the virus on the body, mechanisms of pain, management of chronic pain post-pandemic, and potential treatment options available for people living with chronic pain who have had or are currently infected with COVID-19.
Diagnosing and Managing Uveitis Associated with Immune Checkpoint Inhibitors: A Review
Authors: Zhang Huixin , Houadj Lysa , Wu Kevin Y. , Tran Simon D.
Published: February 2024

This review aims to provide an understanding of the diagnostic and therapeutic challenges of uveitis associated with immune checkpoint inhibitors (ICI). In the wake of these molecules being increasingly employed as a treatment against different cancers, cases of uveitis post-ICI therapy have also been increasingly reported in the literature, warranting an extensive exploration of the clinical presentations, risk factors, and pathophysiological mechanisms of ICI-induced uveitis. This review further provides an understanding of the association between ICIs and uveitis, and assesses the efficacy of current diagnostic tools, underscoring the need for advanced techniques to enable early detection and accurate assessment. Further, it investigates the therapeutic strategies for ICI-related uveitis, weighing the benefits and limitations of existing treatment regimens, and discussing current challenges and emerging therapies in the context of their potential efficacy and side effects. Through an overview of the short-term and long-term outcomes, this article suggests recommendations and emphasizes the importance of multidisciplinary collaboration between ophthalmologists and oncologists. Finally, the review highlights promising avenues for future research and development in the field, potentially informing transformative approaches in the ocular assessment of patients under immunotherapy and the management of uveitis following ICI therapy.
Optical Emission Spectroscopy for the Real-Time Identification of Malignant Breast Tissue
Authors: Guergan Selin , Boeer Bettina , Fugunt Regina , Helms Gisela , Roehm Carmen , Solomianik Anna , Neugebauer Alexander , Nuessle Daniela , Schuermann Mirjam , Brunecker Kristin , Jurjut Ovidiu , Boehme Karen A. , Dammeier Sascha , Enderle Markus D. , Bettio Sabrina , Gonzalez-Menendez Irene , Staebler Annette , Brucker Sara Y. , Kraemer Bernhard , Wallwiener Diethelm , Fend Falko , Hahn Markus
Published: February 2024

Breast conserving resection with free margins is the gold standard treatment for early breast cancer recommended by guidelines worldwide. Therefore, reliable discrimination between normal and malignant tissue at the resection margins is essential. In this study, normal and abnormal tissue samples from breast cancer patients were characterized ex vivo by optical emission spectroscopy (OES) based on ionized atoms and molecules generated during electrosurgical treatment. The aim of the study was to determine spectroscopic features which are typical for healthy and neoplastic breast tissue allowing for future real-time tissue differentiation and margin assessment during breast cancer surgery. A total of 972 spectra generated by electrosurgical sparking on normal and abnormal tissue were used for support vector classifier (SVC) training. Specific spectroscopic features were selected for the classification of tissues in the included breast cancer patients. The average classification accuracy for all patients was 96.9%. Normal and abnormal breast tissue could be differentiated with a mean sensitivity of 94.8%, a specificity of 99.0%, a positive predictive value (PPV) of 99.1% and a negative predictive value (NPV) of 96.1%. For 66.6% patients all classifications reached 100%. Based on this convincing data, a future clinical application of OES-based tissue differentiation in breast cancer surgery seems to be feasible.
Mixed Heterotopic Gastrointestinal/Respiratory Oral Cysts in Newborns: From Prenatal Diagnosis to Histopathological and Therapeutic Management: A Case Report and Literature Review
Authors: Varlas Valentin Nicolae , Parlatescu Ioanina , Epistatu Dragos , Neagu Oana , Varlas Roxana Georgiana , Bălănescu Laura
Published: February 2024

Fetal lingual tumors are very rare, and their early prenatal diagnosis is important for defining the subsequent therapeutic strategy. In this study, we aimed to describe a case of a congenital septate lingual cyst and perform an extensive literature review on two main databases (PubMed, Web of Science), analyzing the clinical manifestations, the imaging appearance, the differential diagnosis, and particularities regarding the treatment of these tumors. The electronic search revealed 17 articles with 18 cases of mixed heterotopic gastrointestinal/respiratory oral epithelial cysts that met the eligibility criteria and were included in this review. The clinical case was diagnosed prenatally during second-trimester screening. On the eighth day of life, the fetus underwent an MRI of the head, which revealed an expansive cystic process on the ventral side of the tongue with the greatest diameter of 21.7 mm, containing a septum of 1 mm inside. On the 13th day of life, surgery was performed under general anesthesia, and the lingual cystic formation was completely excised. The postoperative evolution was favorable. The histopathological examination revealed a heterotopic gastric/respiratory-mixed epithelial cyst with non-keratinized respiratory, gastric squamous, and foveolar epithelium. The lingual cyst diagnosed prenatally is an accidental discovery, the differential diagnosis of which can include several pathologies with different degrees of severity but with a generally good prognosis.
Association between Hospital Prescribed Diets and Length of Stay, Re-Presentation, and Gastrointestinal Symptoms among Acute Uncomplicated Diverticulitis Patients: A Prospective Cohort Study
Authors: Nucera Romina , Jenkins Julie , Crichton Megan , Roberts Shelley , Dalwood Phoebe , Eberhardt Fiona , Mahoney Sophie , Marshall Skye
Published: February 2024

Background and aim: Variation in practice has been observed among the prescribed diets for inpatients with acute, uncomplicated diverticulitis. This study aimed to observe outcomes (length of stay (LOS), re-presentations, and gastrointestinal symptoms) for acute, uncomplicated diverticulitis inpatients prescribed restricted or liberalised diets during usual care. Methods: Adult inpatients with acute, uncomplicated diverticulitis were prospectively enrolled from 2017 to 2019. Demographics, clinical data, and prescribed diets were collected from medical records. Patients were categorised as having restricted or liberalised diets. Primary outcomes were LOS, re-presentations related to diverticulitis, and gastrointestinal symptoms, collected from medical records and patient surveys. Descriptive statistics were used to characterise all variables. Adjusted regression analysis was used to determine the effect of diet prescription on primary outcomes. Results: Of the 81 included patients, 47% were prescribed restricted diets, and 53% were prescribed liberalised diets. Patients prescribed restricted diets had greater LOS (median 4.0 (IQR: 3.5–5.1) vs. 2.8 (IQR: 2.2–3.8) days, p < 0.001) and were more likely to re-present due to diverticulitis at six months (OR 8.1, 95% CI 1.3–51.0, p = 0.025) in adjusted models. No difference in gastrointestinal symptoms was observed 30-days post-admission. Conclusion: In patients hospitalised with uncomplicated diverticulitis, restricted diets may be associated with longer LOS and increased odds of re-presentation at 6 months compared to liberalised diets. No differences in post-discharge gastrointestinal symptoms were observed between restricted versus liberalised diets. Randomised controlled trials are needed to confirm the causal role of inpatient diet prescription on clinical outcomes, healthcare utilisation, and patient experience. Registration: prospectively registered; ANZCTR Number: ACTRN12616001378415.
Problem-Based Learning versus Traditional Learning in Physics Education for Engineering Program Students
Authors: Marcinauskas Liutauras , Iljinas Aleksandras , Čyvienė Jurgita , Stankus Vytautas
Published: February 2024

Problem-based learning (PBL) is an educational method which involves learning by solving real problems. However, applying a PBL approach to engineering subjects in some cases became a challenging issue. Thus, it is important to determine the best ways in which engineering students can learn physics more effectively and solve problems relevant to their daily lives. The main aim of this study was to compare the final assessment grades in a physics course for students in the engineering program using PBL and traditional learning (TL) methods. The advantages and disadvantages of PBL and TL methods from the practical experience of lecturers are also presented. The study presented data of 460 first-year engineering students taking the physics course at Kaunas University of Technology. The information presented in this study is based on the insights, observations, and individual experiences of the authors as teaching staff. The results indicated that the application of the PBL method allows for enhancement in the teamwork, presentation, and critical thinking skills in physics. However, the TL method promotes the individual learning skills and wider theoretical knowledge in physics of students and is more suitable when the exam only includes closed-ended questions.
Using Critical Social Theory as Professional Learning to Develop Scholar—Practitioners in Physical Education: The Example of Bourdieu’s Theory of Practice
Authors: Brown David H. K. , Lloyd Rhys G.
Published: February 2024

In this conceptual article, we present the idea that the physical education (PE) teacher is, out of professional necessity, a scholar—practitioner. To substantiate and evolve this idea, we develop a Deweyian pragmatist view of the scholar—practitioner which also embraces criticality. This stance collapses the binary notion of theory and practice and instead embraces theory and practice as a duality. We add a critical dimension to this duality through recognising that scholar—practitioners (especially leaders) require criticality and cultural awareness to raise questions over knowledge conventions and the mitigations of contextual factors their learners may face. We then turn to the critical and practical sociology of Pierre Bourdieu to illustrate how the use of theory may help PE scholar—practitioners enhance their criticality and cultural awareness. More specifically, we draw on Bourdieu’s primary concepts of field, capital and habitus to show how the application of this theory can help PE scholar—practitioners position themselves in relation to their profession and how they might know their field; be aware of their, and their students’, capital worth in relation to that field; and know themselves (their personal—professional habitus). In conclusion, we suggest that for PE scholar—practitioners, theory-based learning and its applied use might be considered a form of professional learning and not be limited to Bourdieu’s theory but extended to include a range of other aspects of critical social theory. We finish by suggesting that this approach to the PE scholar—practitioner is even more pertinent due to the field of PE becoming increasingly a third space—something which requires additional research and analysis.
Teacher Professional Development in Integrated STEAM Education: A Study on Its Contribution to the Development of the PCK of Physics Teachers
Authors: Martins Iva , Baptista Mónica
Published: February 2024

Integrated STEM education (iSTEM) has attracted attention due to its potentialities regarding students’ learning and intentions to pursue STEM-related careers. However, although increasingly popular, iSTEM remains challenging and elusive, particularly from teachers’ perspective. This scenario became even more complex with the inclusion of “A”, from the Arts. Considering that the quality of teachers is decisive in the success of integrated STEAM education (iSTEAM), it is essential to provide teachers with opportunities to develop their Pedagogical Content Knowledge (PCK) for iSTEAM. In this work, the aim was to understand the effect of teacher professional development (TPD) within iSTEAM on the development of Physics teachers’ PCK related to the topic of “Electrical circuits with associations in series and parallel”. This study followed a pre-test/post-test design with a single group, which facilitates the subsequent comparison of participants’ reported PCK before and after their involvement in the TPD. The results showed that the TPD had a favorable impact on teachers’ PCK. The results of this study also contribute to defining a specific PCK for STEAM (STEAM-PCK).
The Grass Ceiling: Hidden Educational Barriers in Rural England
Authors: Graham Luke
Published: February 2024

Rurality is rarely integrated into analyses of educational inequalities and this article presents an alternative perspective on rural–urban attainment and highlights the impact of rurality on educational outcomes. The traditional narrative of urban–rural educational disadvantage is that urban pupils do less well in the English exam system. Decontextualised data across different English exam performance measures demonstrate how rural pupils outperform their urban counterparts. Socioeconomic disadvantage (SED) has the most significant impact on attainment and this analysis explores the rural–urban attainment gap through this SED lens. An analysis of the Department for Education (DfE) data explores possible factors that might explain the emerging rural educational gap and presents an argument that rurality is another limiting factor that intersects with SED. This article demonstrates how rural underachievement in England has been hidden by the relative sizes and SED distribution of rural and urban populations.
Cooperating Teachers’ Perceptions and Contributions to Preservice Teachers’ Professional Identities
Authors: Amorim Catarina , Ribeiro-Silva Elsa
Published: February 2024

Research on teachers’ socialisation years after they begin teaching in schools has not been extensively undertaken, and there are significant long-term consequences for how these experiences in organisational socialisation influence the quality of physical education programs. The purpose of this study was to determine how cooperating teachers perceive their role in the development of preservice physical education teachers’ professional identities. We used a qualitative methodology, gathering data through semi-structured interviews. Seven cooperating physical education teachers (five female and two male) with between 22 and 40 years of service were interviewed. The data were analysed through thematic content analysis using a constant comparative modality. The interview questions were categorised into three themes: reasons for being a cooperating teacher, the role of the cooperating teacher, and the professional identity of the cooperating teacher. The findings suggest various interpretations of the cooperating teacher’s role and ways of working. The most important skills to have in order to be a cooperating teacher appear to be related to how they were socialised and how they continue to experience the socialisation process during organisational socialisation, as well as the career phase in which they currently find themselves.
Leveraging Swarm Intelligence for Optimal Thermal Camera and Sensor Placement in Industrial Environments
Authors: Zarzycki Hubert , Ewald Dawid , Prokopowicz Piotr
Published: February 2024

This research addresses the sensor placement optimization problem (SPOP) in the industrial sector, aiming to enhance operational efficiency and safety through the strategic deployment of sensors. The focus is on optimizing the locations of thermal cameras and motion sensors, with a dual objective of maximizing coverage and minimizing redundancy in the production hall. To solve this challenge, a method based on the application of the nature-inspired bat algorithm was employed. The study reveals noteworthy findings, emphasizing the proficiency of the bat algorithm (BA) in optimizing the placement of thermal cameras and motion sensors. Numeric outcomes demonstrate the algorithm’s effectiveness in maximizing machine coverage while minimizing sensor usage within a real-world industrial environment. These results underscore the versatility and reliability of the BA, establishing it as a valuable tool for addressing complex optimization tasks in industrial settings.
Testing in Noise Based on the First Adaptive Matrix Sentence Test in Slovak Language
Authors: Kiktová Eva , Sock Rudolph , Getlík Peter
Published: February 2024

This study deals with an acoustic perceptual test performed on the basis of adaptive matrix tests, which represent a modern and reliable tool that can be used not only in perceptual phonetics but also for detecting problems related to hearing. The tests used, based on the first Slovak adaptive matrix, provided extensive test material, which was evaluated through a series of tests implemented according to ICRA (International Collegium of Rehabilitative Audiology) guidelines. Healthy listeners took part in the tests, and, during the tests, they listened to prepared sentence stimuli simultaneously with noise. Out of a total number of 30 tests, 15 tests met the demanding criteria. The tests were evaluated from the point of view of the word recognition score, the slope of the psychometric curve function, and also the threshold values corresponding to word recognition at the levels of 20%, 50%, and 80%. We also investigated and compared the impact of two different testing strategies (open and closed test format) and also the impact of experience or unfamiliarity with the test routine used. The created tests achieved SRT50 = −7.03 ± 0.79 dB and a slope of 13.13 ± 1.60%/dB.
A Reconfigurable Hybrid ADC Using a Jump Search Algorithm
Authors: Oh Sung Kwang , Yoon Kwang Sub , Lee Jonghwan
Published: February 2024

This paper presents a reconfigurable hybrid Analog to Digital Converter (ADC) designed specifically for bio-signal processing, aiming to achieve low power consumption and high area efficiency. The proposed ADC utilizes a combination of 10-bit Most Significant Bit (MSB) Successive Approximation Register (SAR) and 2–4-bit Least Significant Bit (LSB) Single Slope (SS) architectures. The SS architecture incorporates the Dummy Capacitor Quantization Method (DCQM) which employs a 10-bit MSB dummy capacitor. This dummy capacitor can be configured to represent the 2-LSBs or reconstruct 4-LSBs. The reconfigurability of the ADC is achieved through the control of the reset timing of a 5-bit counter enabled by an external signal. The proposed ADC was fabricated using a Complementary Metal Oxide Semiconductor (CMOS) n-well 1-poly 8-metal process. Experimental measurements revealed that the ADC operates at a speed of 454 kS/s with power consumption of 18.7 μW. The Effective Number of Bits (ENoB) achieved by the ADC is 10.9 bits based on a 14-bit scale or 10.2 bits based on a 12-bit scale. The Figure of Merit (FoM) for the ADC is calculated to be 21.5 fJ/step for the 14-bit scale and 22.1 fJ/step for the 12-bit scale.
Specific Emitter Identification Based on Attractor Feature Space of System under Blind Equalization
Authors: Shi Wenqiang , Lei Yingke , Jin Hu , Teng Fei , Lou Caiyi
Published: February 2024

In the process of the individual identification of radiation sources, the effective extraction of fine features of target radiation sources can be regarded as being crucial for the subsequent individual identification. However, in the complex electromagnetic environment, the effective extraction of the radiation source features is still facing great challenges. To solve this problem, we propose an algorithm for constructing the attractor feature space of the radiation source system based on blind equalization to solve this problem. Firstly, we use blind equalization to process the target signal. Secondly, we use the phase-space reconstruction technique to construct the system attractor feature space of the target signal processed, and explore the adaptive relationship between feature-space-embedding dimensions, the delay time and the neural network, finding the optimal values. Finally, the feature space is used as the input of the neural network for the subsequent individual discrimination decision. Experimentally, it is proved that our proposed algorithm improves the individual recognition rate of the target radiation source under the complex electromagnetic environment to a certain extent, which is of practical application value.
Advancing Performance in LoRaWAN Networks: The Circular Region Grouped Bit-Slot LoRa MAC Protocol
Authors: Li Xiaowu , Xu Junjie , Li Runxin , Jia Lianyin , You Jinguo
Published: February 2024

LoRaWAN is an emerging Low-Power Wide-Area Network (LPWAN) technology, widely adopted in various Internet of Things (IoT) applications due to its long transmission range, low power consumption, and robust anti-interference capabilities. However, using the ALOHA medium access control (MAC) protocol in LoRaWAN significantly reduces the packet delivery rate, particularly in high-density networks where end devices (EDs) access the network randomly. It seriously affects the overall network performance. This paper introduces the Circular Region Grouped Bit-Slot CGBS-LoRa MAC protocol to address this challenge. The protocol takes a proactive approach by allocating transmission parameters for end devices, executing regional segmentation based on the distance between EDs and the gateway using different spreading factors. Concurrently, improving the ALOHA access method ensures the efficient communication of EDs in the region. Experimental results demonstrate that our proposed protocol markedly improves the scalability of LoRa networks and minimizes device collisions compared to three other MAC protocols. Even as the LoRaWAN network expands, the proposed protocol maintains a high packet delivery rate and low latency.
Improvement of Industrial Maintenance Plans through Assistance-Driven Reliability-Centered Maintenance and Case-Based Reasoning Design
Authors: Rodríguez-Padial Néstor , Marín Marta M. , Domingo Rosario
Published: February 2024

The present work builds on studies where the industrial market is currently characterized by a highly variable demand in terms of the quantities and flexibility of manufacturing or mass customization, which translates into a more demanding production context in terms of the continuous changes that are required in the production systems, the effect of which results in an increase in the fatigue of the machines that make up the production systems. However, current production systems tend to use highly communicative and sensorized cyber–physical systems; these characteristics can be used to integrate them into decision-assisted systems to improve the availability of the industrial plant. The developed assisted system focuses on collecting and taking advantage of historical knowledge of industrial plant failures and breakdowns. By ideally integrating the reliability-centered maintenance (RCM) methodology and case-based reasoning (CBR) algorithms implemented in a Java application, it is possible to design maintenance plans that are adjusted to the real and changing operational context of any industrial plant. As a result, faster and more accurate decisions are made, because they are based on data. This article focuses on improving certain aspects of the developed assisted system by adding more value by incorporating fuzzy logic (FL) techniques. The aim is to improve the way of entering information about risk factors and their relative importance by incorporating natural language instead of a numerical score, resulting in increased precision in the calculation of the risk priority number (RPN) of the new cases that are incorporated into the assisted system. On the other hand, an attempt has been made to correct two of the main inherent and recognized weaknesses in the classic RPN calculation method by implementing an appropriate mix of fuzzy logic techniques.