Antioxidant Potential of Black, Green and Oolong Tea Methanol Extracts
Authors: Widowati Wahyu , Herlina Tati , Ratnawati Hana , Constantia Gabriella , Deva I Dewa Gde Sathya , Maesaroh Maesaroh 
Published: October 2016

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</xml><![endif]--><!--[if gte mso 10]> <style> /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin:0cm; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:10.0pt; font-family:"Calibri","sans-serif";} </style> <![endif]--><!--[if gte mso 9]><xml> <o:OfficeDocumentSettings> <o:AllowPNG/> </o:OfficeDocumentSettings> </xml><![endif]--><p class="MsoNoSpacing"><span class="hps"><span style="font-size: 9.0pt;">Degenerative diseases</span></span><span class="hps"><span style="font-size: 9.0pt;">and</span></span><span class="hps"><span style="font-size: 9.0pt;">chronic</span></span><span class="hps"><span style="font-size: 9.0pt;">diseases</span></span><span class="hps"><span style="font-size: 9.0pt;">are often</span></span><span class="hps"><span style="font-size: 9.0pt;">caused</span></span><span class="hps"><span style="font-size: 9.0pt;">by</span></span><span class="hps"><span style="font-size: 9.0pt;">oxidative</span></span><span class="hps"><span style="font-size: 9.0pt;">stress</span></span><span style="font-size: 9.0pt;">. Oxidative stress caused </span><span style="font-size: 9.0pt; mso-ansi-language: IN;" lang="IN">by</span><span style="font-size: 9.0pt;"> free radicals. </span><span style="font-size: 9.0pt; mso-ansi-language: IN;" lang="IN">Antixodant as inhibitor are needed t</span><span style="font-size: 9.0pt;">o prevent it</span><span style="font-size: 9.0pt; mso-ansi-language: IN;" lang="IN"> which is </span><span style="font-size: 9.0pt;">one of </span><span style="font-size: 9.0pt; mso-ansi-language: IN;" lang="IN">antioxidant sources </span><span style="font-size: 9.0pt;">is tea. <span class="hps">Tea processing</span></span><span style="font-size: 9.0pt; mso-ansi-language: IN;" lang="IN">generally produce various kinds of teas such as black, green and oolong tea. Tea processing<span style="mso-spacerun: yes;">  </span></span><span class="hps"><span style="font-size: 9.0pt;">affect the</span></span><span class="hps"><span style="font-size: 9.0pt;">content of</span></span><span class="hps"><span style="font-size: 9.0pt;">phenoli</span></span><span class="hps"><span style="font-size: 9.0pt; mso-ansi-language: IN;" lang="IN">c </span></span><span class="hps"><span style="font-size: 9.0pt;">compounds</span></span><span style="font-size: 9.0pt;">.</span><span style="font-size: 9.0pt; mso-ansi-language: IN;"> <span lang="IN">The aim of the research is to evaluate phytochemical content, </span></span><span class="hps"><span style="font-size: 9.0pt;">total</span></span><span class="hps"><span style="font-size: 9.0pt;">phenolic conten</span></span><span class="hps"><span style="font-size: 9.0pt; mso-ansi-language: IN;" lang="IN">t </span></span><span class="hps"><span style="font-size: 9.0pt;">of black</span></span><span class="hps"><span style="font-size: 9.0pt;">tea</span></span><span style="font-size: 9.0pt;">, <span class="hps">green</span></span><span class="hps"><span style="font-size: 9.0pt; mso-ansi-language: IN;" lang="IN"> tea </span></span><span class="hps"><span style="font-size: 9.0pt;">and</span></span><span class="hps"><span style="font-size: 9.0pt;">oolong</span></span><span class="hps"><span style="font-size: 9.0pt;">tea</span></span><span style="font-size: 9.0pt;"> extracts using catechin, quercetin</span><span style="font-size: 9.0pt; mso-ansi-language: IN;" lang="IN">, kaempferol, myricetin as </span><span style="font-size: 9.0pt;">standard, <span class="hps">and to evaluate<em style="mso-bidi-font-style: normal;"> </em></span></span><span style="font-size: 9.0pt; mso-ansi-language: IN;" lang="IN">the antioxidative potency</span><span style="font-size: 9.0pt;"> of black tea, green tea and oolong tea extracts</span><span style="font-size: 9.0pt; mso-ansi-language: IN;" lang="IN"> compared to </span><span style="font-size: 9.0pt;">catechin, quercetin</span><span style="font-size: 9.0pt; mso-ansi-language: IN;" lang="IN">, kaempferol, myricetin</span><span style="font-size: 9.0pt;">. </span><span style="font-size: 9.0pt; mso-ansi-language: IN;" lang="IN">Phytochemical assay using modified Farnsworth method, the antioxidant activity were measured </span><span style="font-size: 9.0pt;">by </span><span style="font-size: 9.0pt; mso-ansi-language: IN;" lang="IN"><span style="mso-spacerun: yes;"> </span>by its 1,1-diphenyl-2-picryl-hydrazyl (DPPH) scavenging activity.<span class="hps"><em style="mso-bidi-font-style: normal;"> </em>Green tea extract contained highest phenolic and flavonoid. </span></span><span class="hps"><span style="font-size: 9.0pt;"><span style="mso-spacerun: yes;"> </span>The highest antioxidant activity was green tea extract with<em style="mso-bidi-font-style: normal;"> IC<sub>50</sub></em></span></span><span style="font-size: 9.0pt;">=0,487</span><span style="font-size: 9.0pt;">μ</span><span style="font-size: 9.0pt; mso-ansi-language: IN;" lang="IN">g/mL</span><span style="font-size: 9.0pt;">.</span><span style="font-size: 9.0pt; mso-ansi-language: IN;" lang="IN"> Green tea extract content phenol and flavonoid are higher compared to the other extracts,<span style="mso-spacerun: yes;">  </span>green tea extract has the highest antioxidant activity.</span></p>