bluuebunny/crossref_metadata_2025 huggingface dataset:
Effectively Elevating Ceramic Fillers’ Dispersity in Gel Hybrid Electrolyte through Bridge–Linked Construction for High–Performance Lithium Metal Batteries
Authors: Chen Minghua , Liu Wannian , Yue Ziyu , Wang Yang , Wu Yixin , Li Yu , Chen Zhen
Published: May 2023

Gel polymer-ceramic hybrid electrolytes (GHEs) have emerged as desirable candidates for preparing high energy density and excellent practicability gel batteries. However, the agglomeration of ceramic particles in polymer matrix leads to a decrease in cycling stability and low mechanical properties of GHEs. Here, we present a feasible method for improving the dispersity of Li0.24La0.59TiO3 (LLTO) nanorods in the polyvinylidenefluoride (PVDF)/poly(propylene carbonate) (PPC) co-blended matrix (K–LLTO/PVDF/PPC) by γ-(2,3-epoxypropoxy)propytrimethoxysilane (KH560) surface treatment. The as-prepared GHE with 10% K–LLTO filler (10% GHE) exhibits a high ionic conductivity (3.01 mS cm−1) and an appropriate lithium-ion transference number (0.55). The Li|10% GHE|Li symmetric cell shows an exceptional lithium stripping-plating lifetime of > 2000 h at 0.1 mA cm−2. The assembled LiFePO4 (LFP)|10% GHE|Li full cells show satisfactory cycling stability in the 2.5–4.2 V electrochemical window by recovering 84% of the initial capacity at 2 C over 500 cycles. The uniformly dispersed K–LLTO within the polymer matrix is ascribed to the formation of a bridge-linked network via Si–O–Ti bonds between KH560 and LLTO, and plenty of hydrogen bonds within the polymer matrix. This modification method provides a feasible strategy for fabricating GHEs with good repeatability, which may easily adapt to the high requirements of commercial production.
Design of Sodium Titanate Nanowires as Anodes for Dual Li,Na Ion Batteries
Authors: Stanchovska Silva , Kalapsazova Mariya , Harizanova Sonya , Koleva Violeta , Stoyanova Radostina
Published: May 2023

The bottleneck in the implementation of hybrid lithium-sodium-ion batteries is the lack of anode materials with a desired rate capability. Herein, we provide an in-depth examination of the Li-storage performance of sodium titanate nanowires as negative electrodes in hybrid Li,Na-ion batteries. Titanate nanowires were prepared by a simple and reproducible hydrothermal method. At a low reaction pressure, the well-isolated nanowires are formed, while by increasing the reaction pressure from 2 to 30 bar, the isolated nanowires tend to bundle. In nanowires, the local coordinations of Na and Ti atoms deviate from those in Na2Ti3O7 and Na2Ti6O13 and slightly depend on the reaction pressure. During the annealing at 350 °C, both Na and Ti coordinations undergo further changes. The nanowires are highly defective, and they easily crystallize into Na2Ti6O13 and Na2Ti3O7 phases. The lithium storage properties are evaluated in lithium-ion cells vs. lithium metal anode and titanate electrodes fabricated with PVDF and carboxymethyl cellulose (CMC) binders. The Li-storage by nanowires proceeds by a hybrid capacitive-diffusive mechanism between 0.1 and 2.5 V, which enables to achieve a high specific capacity. Sodium titanates accommodate Li+ by formation of mixed lithium-sodium-phase Na2−xLixTi6O13, which is decomposed to the distinct lithium phases Li0.54Ti2.86O6 and Li0.5TiO2. Contrary to lithium, the sodium storage is accomplished mainly by the capacitive reactions, and thus the phase composition is preserved during cycling in sodium ion cells. The isolated nanowires outperform bundled nanowires with respect to rate capability.
Massive Parallel Alignment of RNA-seq Reads in Serverless Computing
Authors: Cinaglia Pietro , Vázquez-Poletti José Luis , Cannataro Mario
Published: May 2023

In recent years, the use of Cloud infrastructures for data processing has proven useful, with a computing potential that is not affected by the limitations of a local infrastructure. In this context, Serverless computing is the fastest-growing Cloud service model due to its auto-scaling methodologies, reliability, and fault tolerance. We present a solution based on in-house Serverless infrastructure, which is able to perform large-scale RNA-seq data analysis focused on the mapping of sequencing reads to a reference genome. The main contribution was bringing the computation of genomic data into serverless computing, focusing on RNA-seq read-mapping to a reference genome, as this is the most time-consuming task for some pipelines. The proposed solution handles massive parallel instances to maximize the efficiency in terms of running time. We evaluated the performance of our solution by performing two main tests, both based on the mapping of RNA-seq reads to Human GRCh38. Our experiments demonstrated a reduction of 79.838%, 90.079%, and 96.382%, compared to the local environments with 16, 8, and 4 virtual cores, respectively. Furthermore, serverless limitations were investigated.
Needle-Based Electrical Impedance Imaging Technology for Needle Navigation
Authors: Liu Jan , Atmaca Ömer , Pott Peter Paul
Published: May 2023

Needle insertion is a common procedure in modern healthcare practices, such as blood sampling, tissue biopsy, and cancer treatment. Various guidance systems have been developed to reduce the risk of incorrect needle positioning. While ultrasound imaging is considered the gold standard, it has limitations such as a lack of spatial resolution and subjective interpretation of 2D images. As an alternative to conventional imaging techniques, we have developed a needle-based electrical impedance imaging system. The system involves the classification of different tissue types using impedance measurements taken with a modified needle and the visualization in a MATLAB Graphical User Interface (GUI) based on the spatial sensitivity distribution of the needle. The needle was equipped with 12 stainless steel wire electrodes, and the sensitive volumes were determined using Finite Element Method (FEM) simulation. A k-Nearest Neighbors (k-NN) algorithm was used to classify different types of tissue phantoms with an average success rate of 70.56% for individual tissue phantoms. The results showed that the classification of the fat tissue phantom was the most successful (60 out of 60 attempts correct), while the success rate decreased for layered tissue structures. The measurement can be controlled in the GUI, and the identified tissues around the needle are displayed in 3D. The average latency between measurement and visualization was 112.1 ms. This work demonstrates the feasibility of using needle-based electrical impedance imaging as an alternative to conventional imaging techniques. Further improvements to the hardware and the algorithm as well as usability testing are required to evaluate the effectiveness of the needle navigation system.
The Role of Venetoclax in Relapsed/Refractory Acute Myeloid Leukemia: Past, Present, and Future Directions
Authors: Piccini Matteo , Mannelli Francesco , Coltro Giacomo
Published: May 2023

Relapsed and/or refractory (R/R) acute myeloid leukemia (AML) is hallmarked by dramatic prognosis. Treatment remains challenging, with allogeneic hematopoietic stem cell transplantation (HSCT) as the only curative option. The BCL-2 inhibitor venetoclax (VEN) has proven to be a promising therapy for AML and is currently the standard of care in combination with hypomethylating agents (HMAs) for newly diagnosed AML patients ineligible for induction chemotherapy. Given its satisfactory safety profile, VEN-based combinations are increasingly being investigated as a part of the therapeutic strategy for R/R AML. The current paper aims to provide a comprehensive review of the main evidence regarding VEN in the setting of R/R AML, with a specific focus on combinational strategies, including HMAs and cytotoxic chemotherapy, as well as different clinical settings, especially in view of the crucial role of HSCT. A discussion of what is known about drug resistance mechanisms and future combinational strategies is also provided. Overall, VEN-based regimes (mainly VEN + HMA) have provided unprecedented salvage treatment opportunities in patients with R/R AML, with low extra-hematological toxicity. On the other hand, the issue of overcoming resistance is one of the most important fields to be addressed in upcoming clinical research.
Assessment of Cadmium (Cd) and Lead (Pb) Blood Concentration on the Risk of Endometrial Cancer
Authors: Michalczyk Kaja , Kupnicka Patrycja , Witczak Grzegorz , Tousty Piotr , Bosiacki Mateusz , Kurzawski Mateusz , Chlubek Dariusz , Cymbaluk-Płoska Aneta
Published: May 2023

Background: Cadmium (Cd) and lead (Pb) are heavy metals with carcinogenic potential. Their increased concentration has been correlated with a risk of malignancies, including breast, lung, kidney, gastrointestinal, and gynecological cancers. Most of the studies have evaluated tissue heavy metal concentration. To the best of our knowledge, this is the first study to evaluate blood Cd and lead levels in different uterine pathologies and the risk of endometrial cancer. Methods: This study included 110 patients with a histopathological diagnosis of endometrial cancer, endometrial polyps, endometrial hyperplasia, uterine myoma, and normal endometrium. The patients included in the study were assessed in terms of their endometrial cancer risk factors and blood heavy metal levels. The analysis was conducted using inductively coupled plasma optical emission spectrometry. Results: There was a significant difference in the Cd and Cd/Pb ratio among the different groups of patients (p = 0.002), with higher a median Cd concentration among the endometrial cancer patients. The differences in Pb concentration were not significant (p = 0.717). There were also no differences in the Cd and Pb concentrations based on the patients’ menopausal status nor BMI index. The univariate logistic regression showed a blood cadmium concentration above the median to be associated with an increased risk of endometrial cancer (OR = 5.25; 95% CI 1.56, 17.72). No significant associations were observed between the Pb concentration or Cd/Pb ratio and endometrial cancer risk. Conclusion: The concentration of Cd varies in patients diagnosed with different uterine pathologies. Increased blood cadmium concentration seems to be a risk factor for endometrial studies. Further research on greater populations, accounting for environmental and lifestyle heavy metal exposure, is required to validate our findings.
Favorable Preclinical Pharmacological Profile of a Novel Antimalarial Pyrrolizidinylmethyl Derivative of 4-amino-7-chloroquinoline with Potent In Vitro and In Vivo Activities
Authors: Basilico Nicoletta , Parapini Silvia , D’Alessandro Sarah , Misiano Paola , Romeo Sergio , Dondio Giulio , Yardley Vanessa , Vivas Livia , Nasser Shereen , Rénia Laurent , Russell Bruce M. , Suwanarusk Rossarin , Nosten François , Sparatore Anna , Taramelli Donatella
Published: May 2023

The 4-aminoquinoline drugs, such as chloroquine (CQ), amodiaquine or piperaquine, are still commonly used for malaria treatment, either alone (CQ) or in combination with artemisinin derivatives. We previously described the excellent in vitro activity of a novel pyrrolizidinylmethyl derivative of 4-amino-7-chloroquinoline, named MG3, against P. falciparum drug-resistant parasites. Here, we report the optimized and safer synthesis of MG3, now suitable for a scale-up, and its additional in vitro and in vivo characterization. MG3 is active against a panel of P. vivax and P. falciparum field isolates, either alone or in combination with artemisinin derivatives. In vivo MG3 is orally active in the P. berghei, P. chabaudi, and P. yoelii models of rodent malaria with efficacy comparable, or better, than that of CQ and of other quinolines under development. The in vivo and in vitro ADME-Tox studies indicate that MG3 possesses a very good pre-clinical developability profile associated with an excellent oral bioavailability, and low toxicity in non-formal preclinical studies on rats, dogs, and non-human primates (NHP). In conclusion, the pharmacological profile of MG3 is in line with those obtained with CQ or the other quinolines in use and seems to possess all the requirements for a developmental candidate.
Lymphocyte Subsets and Pulmonary Nodules to Predict the Progression of Sarcoidosis
Authors: Danila Edvardas , Aleksonienė Regina , Besusparis Justinas , Gruslys Vygantas , Jurgauskienė Laimutė , Laurinavičienė Aida , Laurinavičius Arvydas , Mainelis Antanas , Zablockis Rolandas , Zeleckienė Ingrida , Žurauskas Edvardas , Malickaitė Radvilė
Published: May 2023

The search for biological markers, which allow a relatively accurate assessment of the individual course of pulmonary sarcoidosis at the time of diagnosis, remains one of the research priorities in this field of pulmonary medicine. The aim of our study was to investigate possible prognostic factors for pulmonary sarcoidosis with a special focus on cellular immune inflammation markers. A 2-year follow-up of the study population after the initial prospective and simultaneous analysis of lymphocyte activation markers expression in the blood, as well as bronchoalveolar lavage fluid (BALF) and lung biopsy tissue of patients with newly diagnosed pulmonary sarcoidosis, was performed. We found that some blood and BAL fluid immunological markers and lung computed tomography (CT) patterns have been associated with a different course of sarcoidosis. We revealed five markers that had a significant negative association with the course of sarcoidosis (worsening pulmonary function tests and/or the chest CT changes)—blood CD4+CD31+ and CD4+CD44+ T lymphocytes, BALF CD8+CD31+ and CD8+CD103+ T lymphocytes and a number of lung nodules on chest CT at the time of the diagnosis. Cut-off values, sensitivity, specificity and odds ratio for predictors of sarcoidosis progression were calculated. These markers may be reasonable predictors of sarcoidosis progression.
Angiogenesis in the Outer Membrane of Chronic Subdural Hematomas through Thrombin-Cleaved Osteopontin and the Integrin α9 and Integrin β1 Signaling Pathways
Authors: Osuka Koji , Ohmichi Yusuke , Ohmichi Mika , Honma Satoru , Suzuki Chiharu , Aoyama Masahiro , Iwami Kenichiro , Watanabe Yasuo , Miyachi Shigeru
Published: May 2023

Background: A chronic subdural hematoma (CSDH) is considered to be an inflammatory and angiogenic disease. The CSDH outer membrane, which contains inflammatory cells, plays an important role in CSDH development. Osteopontin (OPN) is an extracellular matrix protein that is cleaved by thrombin, generating the N-terminal half of OPN, which is prominently involved in integrin signal transduction. We explored the expression of the N-terminal half of OPN in CSDH fluid and the expression of integrins α9 and β1 and the downstream components of the angiogenic signaling pathways in the outer membrane of CSDHs. Methods: Twenty samples of CSDH fluid and eight samples of CSDH outer membrane were collected from patients suffering from CSDHs. The concentrations of the N-terminal half of OPN in CSDH fluid samples were measured using ELISA kits. The expression levels of integrins α9 and β1, vinculin, talin-1, focal adhesion kinase (FAK), paxillin, α-actin, Src and β-actin were examined by Western blot analysis. The expression levels of integrins α9 and β1, FAK and paxillin were also examined by immunohistochemistry. We investigated whether CSDH fluid could activate FAK in cultured endothelial cells in vitro. Results: The concentration of the N-terminal half of OPN in CSDH fluid was significantly higher than that in the serum. Western blot analysis confirmed the presence of these molecules. In addition, integrins α9 and β1, FAK and paxillin were localized in the endothelial cells of vessels within the CSDH outer membrane. FAK was significantly phosphorylated immediately after treatment with CSDH fluid. Conclusion: Our data suggest that the N-terminal half of OPN in CSDH fluid promotes neovascularization in endothelial cells through integrins α9 and β1. The N-terminal half of OPN, which is part of the extracellular matrix, plays a critical role in the promotion of CSDHs.
The Transcriptomic Landscape of Age-Induced Changes in Human Visceral Fat and the Predicted Omentum-Liver Connectome in Males
Authors: Moraes Diogo de , Mousovich-Neto Felippe , Cury Sarah Santiloni , Oliveira Jakeline , Souza Jeferson dos Santos , Freire Paula Paccielli , Dal-Pai-Silva Maeli , Mori Marcelo Alves da Silva , Fernandez Geysson Javier , Carvalho Robson Francisco
Published: May 2023

Aging causes alterations in body composition. Specifically, visceral fat mass increases with age and is associated with age-related diseases. The pathogenic potential of visceral fat accumulation has been associated with its anatomical location and metabolic activity. Visceral fat may control systemic metabolism by secreting molecules that act in distal tissues, mainly the liver, through the portal vein. Currently, little is known about age-related changes in visceral fat in humans. Aiming to identify molecular and cellular changes occurring with aging in the visceral fat of humans, we analyzed publicly available transcriptomic data of 355 omentum samples from the Genotype-Tissue Expression portal (GTEx) of 20–79-year-old males and females. We identified the functional enrichment of genes associated with aging, inferred age-related changes in visceral fat cellularity by deconvolution analysis, profiled the senescence-associated secretory phenotype of visceral adipose tissue, and predicted the connectivity of the age-induced visceral fat secretome with the liver. We demonstrate that age induces alterations in visceral fat cellularity, synchronous to changes in metabolic pathways and a shift toward a pro-inflammatory secretory phenotype. Furthermore, our approach identified candidates such as ADIPOQ-ADIPOR1/ADIPOR2, FCN2-LPR1, and TF-TFR2 to mediate visceral fat-liver crosstalk in the context of aging. These findings cast light on how alterations in visceral fat with aging contribute to liver dysfunction and age-related disease etiology.
Inhibition of Multidrug Efflux Pumps Belonging to the Major Facilitator Superfamily in Bacterial Pathogens
Authors: Varela Manuel F. , Stephen Jerusha , Bharti Deeksha , Lekshmi Manjusha , Kumar Sanath
Published: May 2023

Bacterial pathogens resistant to multiple structurally distinct antimicrobial agents are causative agents of infectious disease, and they thus constitute a serious concern for public health. Of the various bacterial mechanisms for antimicrobial resistance, active efflux is a well-known system that extrudes clinically relevant antimicrobial agents, rendering specific pathogens recalcitrant to the growth-inhibitory effects of multiple drugs. In particular, multidrug efflux pump members of the major facilitator superfamily constitute central resistance systems in bacterial pathogens. This review article addresses the recent efforts to modulate these antimicrobial efflux transporters from a molecular perspective. Such investigations can potentially restore the clinical efficacy of infectious disease chemotherapy.
Parameter Optimization and DEM Simulation of Bionic Sweep with Lower Abrasive Wear Characteristics
Authors: Wang Shuo , Liu Xuanting , Tong Tianjian , Xu Zihe , Ma Yunhai
Published: May 2023

High wear rates during the tillage process often result in significant financial losses and wasted farming seasons. In this paper, a bionic design was used to reduce tillage wear. Inspired by wear-resistant animals with ribbed structures, the bionic ribbed sweep (BRS) was designed by combining a ribbed unit with a conventional sweep (CS). BRSs with different parameters (width φ, height h, angle θ, and interval λ) were simulated and optimized using the DEM and RSM methods at a working depth of 60 mm to evaluate the magnitude and trends of three responses: tillage resistance (TR), number of contacts between the sweep and soil particles (CNSP), and Archard wear value (AW). The results showed that a protective layer could be created on the surface of the sweep with a ribbed structure to reduce abrasive wear. Analysis of variance proved that factors φ, θ, and λ had significant effects on AW, CNSP, and TR, while factor h was insignificant. An optimal solution was obtained using the desirability method, including 8.88 mm φ, 1.05 mm h, 3.01 mm λ, and 34.46° θ. Wear tests and simulations showed that wear loss could be effectively reduced at different speeds by the optimized BRS. It was found to be feasible to create a protective layer to reduce partial wear by optimizing the parameters of the ribbed unit.
Biomolecular Monitoring Tool Based on Lab-on-Chip for Virus Detection
Authors: Costantini Francesca , Lovecchio Nicola , Nandimandalam Manasa , Manglli Ariana , Faggioli Francesco , Biasin Mara , Manetti Cesare , Roversi Pio Federico , Nascetti Augusto , de Cesare Giampiero , Caputo Domenico
Published: May 2023

Lab-on-Chip (LoC) devices for performing real-time PCR are advantageous compared to standard equipment since these systems allow to conduct in-field quick analysis. The development of LoCs, where the components for performing the nucleic acid amplification are all integrated, can be an issue. In this work, we present a LoC-PCR device where thermalization, temperature control and detection elements are all integrated on a single glass substrate named System-on-Glass (SoG) obtained using metal thin-film deposition. By using a microwell plate optically coupled with the SoG, real-time reverse transcriptase PCR of RNA extracted from both a plant and human virus has been carried out in the developed LoC-PCR device. The limit of detection and time of analysis for the detection of the two viruses by using the LoC-PCR were compared with those achieved by standard equipment. The results showed that the two systems can detect the same concentration of RNA; however, the LoC-PCR performs the analysis in half of the time compared to the standard thermocycler, with the advantage of the portability, leading to a point-of-care device for several diagnostic applications.
Permanent Ad-lib Feeders Decrease the Survival of Wintering Great Tits (Parus major)
Authors: Krama Tatjana , Krams Ronalds , Popovs Sergejs , Trakimas Giedrius , Rantala Markus J. , Freeberg Todd M. , Krams Indrikis A.
Published: May 2023

The optimal body mass hypothesis posits that the body reserves of wintering birds are balanced between the risk of starvation and predation. In this study, we tested whether the body mass of wintering Great Tits (Parus major) was higher under conditions of less predictable food resources. We compared body mass, body mass index, the speed at take-off, and apparent survival of Great Tit adult males wintering in small urban areas either near feeders providing permanent access to food for months or near feeders providing irregular access to food. Body mass and body mass index were greater, while take-off speed and apparent survival were lower, in birds wintering near permanent feeders than birds wintering near irregular feeders. Thus, urban birds, with their predictable access to high energy food, did not follow the fattening strategy predicted by the optimal body mass hypothesis. This study shows that regular excess amounts of high-energy food may affect urban birds’ physiological and behavioral strategies in a non-adaptive way. We recommend irregular feeding of wintering birds and the placing of feeders in places that are safe against attacking predators.
Playing Closer: Using Virtual Reality to Measure Approach Bias of Internet Gaming Disorder
Authors: Wei Wen , Wang Qi , Ding Ruyi , Dong Rui , Ni Shiguang
Published: May 2023

Playing online games is gradually becoming mainstream entertainment, but some people may develop Internet gaming disorder (IGD). Like other behavioral addictive diseases, one of the main characteristics of IGD is a craving for games, which also makes people tend to approach game-related clues. Recently, a few researchers have started to use the approach–avoidance task (AAT) paradigm to study the approach bias of IGD, and they also think it is an essential characteristic of IGD. However, the traditional AAT cannot provide realistic approach–avoidance behavior to stimuli, and virtual reality has been proven to provide a highly ecological environment to measure approach bias. Therefore, this study innovatively integrates virtual reality and the AAT paradigm to measure the approach bias of IGD. We found that compared with neutral stimuli, IGD spent less time approaching game-related stimuli, which indicates that it is difficult for IGD to avoid game-related situations in the virtual environment. This study also revealed that game-related content stimuli in virtual reality alone did not increase the IGD group’s craving for games. These results proved that AAT in VR could cause the approach bias of IGD and provide high ecological validity and an effective tool for the intervention of IGD in the future.
Evaluation Method and Influence Law of UV-Cured Polyurethane on the Self-Healing Performance of Asphalt and Asphalt Mixtures
Authors: Sun Huaizhang , Liu Wenyi , Wang Yu , Chang Xinyue , Zhao Hu , Shi Shengkang , Xing Jianbao , Wu Di , Zhang Jizhe , Zhang Wengang
Published: May 2023

To explore the effect of UV-curable polyurethane (UV-PU) on the self-healing performance (SHP) of asphalt and asphalt mixtures, this article conducted research in this area of the evaluation method and influenced the law. polyurethane (PU), Styrene-Butadiene-Styrene Block Copolymer (SBS Ι-C) and 70# asphalt and asphalt mixtures were introduced as the example of comparison. A method for evaluating the SHP of asphalt, sand asphalt mixture, and medium particle asphalt mixture using the healing index (HI) as an evaluation index was proposed, and the healing performance of various materials under different healing conditions was tested and analyzed. The research results indicate that, HI based on asphalt complex shear modulus (HIA), HI based on sand type asphalt mixture (AC-5) flexural tensile strength (HIAC-5), and HI based on medium grained asphalt mixture (AC-16) bending stiffness modulus (S) HIAC-16 can effectively reflect the SHP of asphalt, AC-5, and AC-16 under different healing conditions, and can be used as an evaluation indicator for SHP. The asphalt SHP is related to the asphalt type and the degree of initial loss and is highly correlated with the healing time. Compared to other asphalt types, the sensitivity of UV-PU or PU modified asphalt to initial loss is lower than that of SBS Ι-C modified asphalt, but higher than 70# asphalt. Under the same light healing time and initial degree of loss, the SHP of UV-PU modified asphalt AC-5 and AC-16 is significantly superior to other types of asphalt mixtures. Compared to asphalt, the self-healing time of AC-5 grade AC-16 is longer. The SHP of AC-16 is highly correlated with the loss degree associated with the initial S. The larger the initial loss degree of the S, the greater the loss rate of the HI.
Passive Control of Ultra-Span Twin-Box Girder Suspension Bridges under Vortex-Induced Vibration Using Tuned Mass Dampers: A Sensitivity Analysis
Authors: Hosseini Lavassani Seyed Hossein , Kontoni Denise-Penelope N. , Alizadeh Hamed , Gharehbaghi Vahidreza
Published: May 2023

Suspension bridges’ in-plane extended configuration makes them vulnerable to wind-induced vibrations. Vortex shedding is a kind of aerodynamic phenomenon causing a bridge to vibrate in vertical and torsional modes. Vortex-induced vibrations disturb the bridge’s serviceability limit, which is not favorable, and in the long run, they can cause fatigue damage. In this condition, vibration control strategies seem to be essential. In this paper, the performance of a tuned mass damper (TMD) is investigated under the torsional vortex phenomenon for an ultra-span streamlined twin-box girder suspension bridge. In this regard, the sensitivity of TMD parameters was addressed according to the torsional responses of the suspension bridge, and the reached appropriate ranges are compared with the outputs provided by genetic algorithm. The results indicated that the installation of three TMDs could control all the vulnerable modes and reduce the torsional rotation by up to 34%.
Mechanical Behavior of Fully Grouted Rock Bolts in Hydraulic Tunnels Subjected to Elevated Ground Temperatures
Authors: Jiang Haibo , Li Shuangxi , Li Qinglin , Xu Juncai
Published: May 2023

In this study, the mechanical behavior of fully grouted rock bolts in hydraulic tunnels subjected to elevated ground temperatures was investigated. A differential equation for axial displacement of the rock bolt was formulated, which considers the force equilibrium of infinitesimal bolt segments and the stress transfer mechanism at the anchor–rock interface. The distribution functions for axial stress within the bolt and the interfacial shear stress were obtained by solving the differential equation, which incorporated the displacement of the surrounding rock mass as a parameter. This study showed that the effectiveness of the bolt–shotcrete support system decreases over time, considering the displacement relaxation rate of the surrounding rock mass. The mechanical model’s variation laws at 20 °C, 50 °C, and 80 °C were summarized by integrating the thermal deformation equation for material parameters, and the numerical simulation results were compared and analyzed. The findings revealed that the bond strength between the rock bolt and the rock mass diminishes as the temperature of the surrounding rock increases, leading to a reduction of interfacial shear stress at both extremities of the bolt. Moreover, the maximum axial force within the bolt escalates as the neutral point migrates farther from the tunnel wall.
Clinical and Histopathological Features of an Italian Monocentric Series of Primary Small Bowel T-Cell Lymphomas
Authors: Lucioni Marco , Fraticelli Sara , Santacroce Giovanni , Bonometti Arturo , Aronico Nicola , Sciarra Roberta , Lenti Marco Vincenzo , Bianchi Paola Ilaria , Neri Giuseppe , Feltri Monica , Neri Benedetto , Ferrario Giuseppina , Riboni Roberta , Corazza Gino Roberto , Vanoli Alessandro , Arcaini Luca , Paulli Marco , Di Sabatino Antonio
Published: May 2023

The gastrointestinal (GI) tract is the most common extranodal site of occurrence of non-Hodgkin lymphomas. Most GI lymphomas are of B-cell lineage, while T-cell lymphomas are less frequent. The aim of our retrospective study was to depict the clinical–pathological profile of a series of patients affected by intestinal T-cell lymphomas (ITCL) and possibly define hallmarks of these neoplasms. A total of 28 patients were included: 17 enteropathy-associated T-cell lymphomas (EATL), 5 monomorphic epitheliotropic T-cell lymphomas (MEITL), 3 indolent T-cell lymphoproliferative disorders of the gastrointestinal tract (ITCLDGT), and 3 intestinal T-cell lymphomas not otherwise specified (ITCL-NOS). Celiac disease (CD) was diagnosed in around 70% of cases. Diagnosis of EATL showed a significant correlation with CD30 expression, whereas MEITL with angiotropism and CD56 positivity. ITCLDGT cases showed plasma cells infiltration. Peripheral lymphocytosis, the absence of a previous diagnosis of CD, an advanced Lugano clinical stage, and the histological subtype ITCL-NOS were significantly associated with worse survival at multivariate analysis. Our findings about the epidemiological, clinical, and histopathological features of ITCL were in line with the current knowledge. Reliable prognostic tools for these neoplasms are still lacking but according to our results lymphocytosis, diagnosis of CD, Lugano clinical stage, and histological subtype should be considered for patient stratification.
Mesoporous Chromium Catalysts Templated on Halloysite Nanotubes and Aluminosilicate Core/Shell Composites for Oxidative Dehydrogenation of Propane with CO2
Authors: Melnikov Dmitry , Smirnova Ekaterina , Reshetina Marina , Novikov Andrei , Wang Hongqiang , Ivanov Evgenii , Vinokurov Vladimir , Glotov Aleksandr
Published: May 2023

The oxidative dehydrogenation of alkanes is a prospective method for olefins production. CO2-assisted propane dehydrogenation over metal oxide catalysts provides an opportunity to increase propylene production with collateral CO2 utilization. We prepared the chromia catalysts on various mesoporous aluminosilicate supports, such as halloysite nanotubes, nanostructured core/shell composites of MCM-41/halloysite (halloysite nanotubes for the core; silica of MCM-41-type for the shell), and MCM-41@halloysite (silica of MCM-41-type for the core; halloysite nanotubes for the shell). The catalysts have been characterized by X-ray fluorescence analysis, low-temperature nitrogen adsorption, X-ray diffraction, temperature-programmed reduction, temperature-programmed desorption of ammonia, transmission electron microscopy with energy-dispersive X-ray spectroscopy, and thermogravimetric analysis. The catalysts’ performance in carbon-dioxide-assisted propane dehydrogenation has been estimated in a fixed-bed reactor at atmospheric pressure. The most stable catalyst is Cr/halloysite, having the lowest activity and the largest pore diameter. The catalyst, Cr/MCM-41/HNT, shows the best catalytic performance: having the highest conversion (19–88%), selectivity (83–30%), and space–time yield (4.3–7.1 mol C3H6/kg catalyst/h) at the temperature range of 550–700 °C. The highest space–time yield could be related to the uniform distribution of the chromia particles over the large surface area and narrow pore size distribution of 2–4 nm provided by the MCM-41-type silica and transport channels of 12–15 nm from the halloysite nanotubes.
Rational Design of Cyclodextrin Glycosyltransferase with Improved Hesperidin Glycosylation Activity
Authors: Chen Hanchi , Wang Jiajun , Liu Yi , Chen Yongfan , Wang Chunfeng , Zhu Linjiang , Lu Yuele , Chen Xiaolong
Published: May 2023

Cyclodextrin glycosyltransferase (CGTase) can catalyze the glycosylation of hesperidin, resulting in α-glycosyl hesperidin with significantly improved water solubility. In this study, a rational design of CGTase to improve its hesperidin glycosylation activity was investigated. The strategy we employed involved docking hesperidin in its near-attack conformation and virtually mutating the surrounding residues, followed by calculating the changes in binding energy using Rosetta flex-ddG. The mutations with a stabilization effect were then subjected to an activity assay. Starting from CGTase-Y217F, we obtained three double-point mutants, Y217F/M351F, Y217F/M351L, and Y217F/D393H, with improved hesperidin glycosylation activities after screening twenty variants. The best variant, Y217F/D393H, exhibited a catalytic activity of 1305 U/g, and its kcat/KmA is 2.36 times higher compared to CGTase-Y217F and 15.14 times higher compared to the wild-type CGTase. Molecular dynamic simulations indicated that hesperidin was repulsed by CGTase-Y217F when bound in a near-attack conformation. However, by introducing a second-point mutation with a stabilization effect, the repulsion effect is weakened, resulting in a reduction in the distances between the bond-forming atoms and, thus, favoring the reaction.
MicroRNAs in Extracellular Vesicles of Alzheimer’s Disease
Authors: Li Wanran , Zheng Yun
Published: May 2023

Alzheimer’s disease (AD) is a neurodegenerative disease with dysfunction of memory, language and thinking. More than 55 million people were diagnosed with AD or other dementia around the world in 2020. The pathology of AD is still unclear and there are no applicable therapies for AD. MicroRNAs (miRNAs) play key roles in AD pathology and have great potential for the diagnosis and treatment of AD. Extracellular vesicles (EVs) widely exist in body fluids such as blood and cerebrospinal fluid (CSF) and contain miRNAs that are involved in cell-to-cell communication. We summarized the dysregulated miRNAs in EVs derived from the different body fluids of AD patients, as well as their potential function and application in AD. We also compared these dysregulated miRNAs in EVs to those in the brain tissues of AD patients aiming to provide a comprehensive view of miRNAs in AD. After careful comparisons, we found that miR-125b-5p and miR-132-3p were upregulated and downregulated in several different brain tissues of AD and EVs of AD, respectively, suggesting their value in AD diagnosis based on EV miRNAs. Furthermore, miR-9-5p was dysregulated in EVs and different brain tissues of AD patients and had also been tested as a potential therapy for AD in mice and human cell models, suggesting that miR-9-5p could be used to design new therapies for AD.
Is There a Difference in the Proteomic Profile of Stimulated and Unstimulated Saliva Samples from Pregnant Women with/without Obesity and Periodontitis?
Authors: Foratori-Junior Gerson Aparecido , Ventura Talita Mendes Oliveira , Grizzo Larissa Tercilia , Jesuino Bruno Gualtieri , Castilho Ana Virgínia Santana Sampaio , Buzalaf Marília Afonso Rabelo , Sales-Peres Silvia Helena de Carvalho
Published: May 2023

This study aimed to compare the proteomic profile of stimulated and unstimulated saliva samples from pregnant women with/without obesity and periodontitis. Pregnant women were allocated into four groups: with obesity and periodontitis (OP); with obesity but without periodontitis (OWP); with normal BMI but with periodontitis (NP); with normal BMI and without periodontitis (NWP). Stimulated saliva (SS) and unstimulated saliva (US) samples were collected, and salivary proteins were extracted and individually processed by proteomic analysis (nLC-ESI-MS/MS). Proteins involved with the immune response process, antioxidant activity, and retina homeostasis were decreased or absent in SS samples from all groups (i.e., Antileukoproteinase, Lysozyme C, Alpha-2-macroglobulin-like protein 1, Heat shock proteins—70 kDa 1-like, 1A, 1B, 6, Heat shock-related 70 kDa protein 2, Putative Heat shock 70 kDa protein 7, Heat shock cognate 71 kDa). Additionally, proteins related to the carbohydrate metabolic process and glycolytic and glucose metabolic process were absent in SS, mainly from OP and OWP (i.e., Frutose-bisphosphate aldose A, Glusoce-6-phosphate isomerase, Pyruvate kinase). Saliva stimulation decreased important proteins involved with immune response and inflammation process in all groups. Unstimulated salivary samples seem to be the best choice for the proteomic approach in pregnant women.
Potential of Oral Cavity Stem Cells for Bone Regeneration: A Scoping Review
Authors: Alarcón-Apablaza Josefa , Prieto Ruth , Rojas Mariana , Fuentes Ramón
Published: May 2023

Bone loss is a common problem that ranges from small defects to large defects after trauma, surgery, or congenital malformations. The oral cavity is a rich source of mesenchymal stromal cells (MSCs). Researchers have documented their isolation and studied their osteogenic potential. Therefore, the objective of this review was to analyze and compare the potential of MSCs from the oral cavity for use in bone regeneration. Methods: A scoping review was carried out following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses extension for Scoping Reviews (PRISMA-ScR) guidelines. The databases reviewed were PubMed, SCOPUS, Scientific Electronic Library Online (SciELO), and Web of Science. Studies using stem cells from the oral cavity to promote bone regeneration were included. Results: A total of 726 studies were found, of which 27 were selected. The MSCs used to repair bone defects were (I) dental pulp stem cells of permanent teeth, (II) stem cells derived from inflamed dental pulp, (III) stem cells from exfoliated deciduous teeth, (IV) periodontal ligament stem cells, (V) cultured autogenous periosteal cells, (VI) buccal fat pad-derived cells, and (VII) autologous bone-derived mesenchymal stem cells. Stem cells associate with scaffolds to facilitate insertion into the bone defect and to enhance bone regeneration. The biological risk and morbidity of the MSC-grafted site were minimal. Successful bone formation after MSC grafting has been shown for small defects with stem cells from the periodontal ligament and dental pulp as well as larger defects with stem cells from the periosteum, bone, and buccal fat pad. Conclusions: Stem cells of maxillofacial origin are a promising alternative to treat small and large craniofacial bone defects; however, an additional scaffold complement is required for stem cell delivery.
Redefining Neurodevelopmental Impairment: Perspectives of Very Preterm Birth Stakeholders
Authors: Synnes Anne , Chera Amarpreet , Richter Lindsay L. , Bone Jeffrey N. , Bourque Claude Julie , Zhang-Jiang Sofia , Pearce Rebecca , Janvier Annie , Luu Thuy Mai
Published: May 2023

Children born very preterm are at risk of severe neurodevelopmental impairment, a composite endpoint that includes cerebral palsy, developmental delay, and hearing and visual impairment defined by medical professionals. We aimed to describe preterm birth stakeholders’ perspectives on this classification. Ten clinical scenarios describing 18-month-old children with different components of severe neurodevelopmental impairment and one scenario of a typically developing child (control) were distributed to parents and stakeholders using a snowball sampling technique. For each scenario, participants rated health on a scale from 0 to 10 and whether the scenario represented a severe condition. Results were analyzed descriptively and mean differences from the control scenario were compared using a linear mixed-effects model. Stakeholders (number = 827) completed 4553 scenarios. Median health scores for each scenario varied from 6 to 10. The rating for the cerebral palsy and language delay scenario was significantly lower (mean difference −4.3; 95% confidence interval: −4.4, −4.1) than the control. The proportion of respondents rating a scenario as “severe” ranged from 5% for cognitive delay to 55% for cerebral palsy and language delay. Most participants disagreed with the rating used in research to describe severe neurodevelopmental impairment in preterm children. The term should be redefined to align with stakeholder perceptions.
New Optimization Strategies on Radiation Protection in Fluoroscopy-Guided Interventional Procedures in Pediatrics
Authors: Ubeda Carlos
Published: May 2023

The term fluoroscopically guided interventional procedure describes a clinical practice in medicine, where fluoroscopic systems are used to conduct diagnostic procedures or provide image guidance for therapeutic interventional procedures performed via percutaneous or other access routes [...]
Juvenile Osteochondral Lesions of the Talus: Current Concepts Review and an Update on the Literature
Authors: Anastasio Albert , Bagheri Kian , Peairs Emily , Grant Caitlin , Adams Samuel
Published: May 2023

Osteochondral lesions of the talus (OLTs) are lesions that occur before the physis closes and are frequently associated with acute ankle trauma. These lesions are often difficult to diagnose due to swelling and inflammation that are present after the initial injury. A growing body of literature has assessed the effects of OLTs in the adult population. However, the literature examining these lesions in the juvenile population is sparse. The purpose of this review is to provide a thorough understanding of OLTs, with a specific focus on the juvenile population. We evaluate the recent literature regarding the outcomes of various surgical treatment; modalities in the pediatric patient. While the outcomes after surgical treatment of pediatric OLTs are generally favorable, the paucity of investigation in this demographic is alarming. Further research is needed to better inform practitioners and families regarding these outcomes, as treatment plans are highly dependent on the individual patient in question.
Al2O3 Ceramic/Nanocellulose-Coated Non-Woven Separator for Lithium-Metal Batteries
Authors: Shin Dong-Min , Son Hyunsu , Park Ko Un , Choi Junyoung , Suk Jungdon , Kang Eun Seck , Kim Dong-Won , Kim Do Youb
Published: May 2023

Separators play an essential role in lithium (Li)-based secondary batteries by preventing direct contact between the two electrodes and providing conduction pathways for Li-ions in the battery cells. However, conventional polyolefin separators exhibit insufficient electrolyte wettability and thermal stability, and in particular, they are vulnerable to Li dendritic growth, which is a significant weakness in Li-metal batteries (LMBs). To improve the safety and electrochemical performance of LMBs, Al2O3 nanoparticles and nanocellulose (NC)-coated non-woven poly(vinylidene fluoride)/polyacrylonitrile separators were fabricated using a simple, water-based blade coating method. The Al2O3/NC-coated separator possessed a reasonably porous structure and a significant number of hydroxyl groups (-OH), which enhanced electrolyte uptake (394.8%) and ionic conductivity (1.493 mS/cm). The coated separator also exhibited reduced thermal shrinkage and alleviated uncontrollable Li dendritic growth compared with a bare separator. Consequently, Li-metal battery cells with a LiNi0.8Co0.1Mn0.1O2 cathode and an Al2O3/NC-coated separator using either liquid or solid polymer electrolytes exhibited improved rate capability, cycle stability, and safety compared with a cell with a bare separator. The present study demonstrates that combining appropriate materials in coatings on separator surfaces can enhance the safety and electrochemical performance of LMBs.
Growth of Nanostructured TiO2 Thin Films onto Lignocellulosic Fibers through Reactive DC Magnetron Sputtering: A XRD and SEM Study
Authors: Eleutério Telmo , Sério Susana , Vasconcelos Helena C.
Published: May 2023

TiO2 thin films were deposited on ginger lily (Hedychium gardnerianum) fibers using a custom-made DC reactive magnetron sputtering system with Ar/O2 mixture at two O2/(O2 + Ar) ratios (50% O2 and 75% O2) and sputtering powers (500 and 1000 W), and their effects on the structure and surface morphology of TiO2 films were investigated. XRD analysis showed the presence of the mainly anatase phase in the deposited films, with a small fraction of rutile phase detected for TiO2 deposited with the higher oxygen percentage and sputtering power. SEM imaging revealed that the films exhibited distinct surface features depending on the deposition conditions. Specifically, films deposited with 50 O2 % and 1000 W exhibited porosity, while the films deposited under other conditions appeared either dense with a cauliflower-like appearance or displayed surface features resembling lines and mountain ranges of coalesced particles. The grain size of dense films increased with increasing sputtering power. The deposition conditions significantly affected the resulting surface topography, with an increase in surface roughness parameters observed for both power levels when the oxygen concentration in the deposition atmosphere was increased from 50% to 75%. The adhesion tests conducted using sonication and EDS analysis revealed that almost all of the studied films exhibited good adhesion, as evidenced by the atomic content (at. %) of Ti remaining intact after sonication, indicating good adhesion. However, the porous film exhibited a slightly lower adhesion grade, suggesting that the porous structure may have influenced the adhesion properties.