bluuebunny/crossref_metadata_2025 huggingface dataset:
Natural Plant Compounds as ANGPTL2 Inhibitors in Glioblastoma: An Integrated Bioinformatics and Molecular Docking Analysis
Authors: POLEBOYINA PAVAN KUMAR , Poleboyina Sneha Malleswari , Pawar Smita
Published: November 2023

Glioblastoma is a primary brain tumor notorious for its extreme aggressiveness and inevitability of recurrence, resulting in a dire prognosis. Angiopoietin-like proteins comprise eight glycoproteins that are secreted and share structural similarities with angiopoietin family proteins. The researchers discovered that the expression of ANGPTL2 is significantly elevated in GBM tumors and that this elevation correlates with the proliferation and dissemination of glioma cells. In drug discovery, Natural plant compounds derived from plants have shown properties that can inhibit the activity of cancer cells. These compounds are capable of inhibiting cancer cell proliferation and promoting apoptosis. In our study, we conducted an integrated bioinformatics analysis to investigate the role of ANGPTL2 in GBM. We utilized natural plant compound inhibitors to identify the plausible inhibitor targeting ANGPTL2. Due to the unavailability of the 3D structure of ANGPTL2 in the Protein Data Bank, we utilized homology modeling using I-TASSER to create a 3D protein model of ANGPTL2. Molecular docking studies were conducted and the highest-scoring ligand was identified. Multiple parameters were utilized in the analysis of protein-ligand interactions. According to our findings, 22-epicalamistrin is a promising potential ANGPTL2 inhibitor for reducing ANGPTL2 expression and halting the progression of Gliomas.
Intention to Utilize Mental Health and Suicide Prevention Resources in a Community Sample During the First Year of the COVID-19 Pandemic
Authors: Cohen Lisa , Hayek Rawad El , Imbastaro Benedetta , Goncearenco Inna , Zheng Sifan , Rogers Megan L. , Pompili Maurizio , Galynker Igor
Published: November 2023

Objective
Given the stressors experienced during the COVID-19 pandemic, it is critical to identify populations with elevated mental health needs during this crisis. This study investigated demographic correlates of reported intention to utilize mental health (MH) and suicide prevention (SP) resources in a community sample during the COVID-19 pandemic.
Methods
A sample of 1,978 adults in the United States completed an anonymous online survey between June 2020 and February 2021.
Results
Intent to utilize MH resources was associated with younger age, single marital status, female gender, and Hispanic vs. White race/ethnicity. Intent to utilize SP resources was associated with younger age, single marital status, and was greater among Black and Hispanic vs. White race/ethnicity. Lower education was associated with MH and SP utilizers in bivariate analysis. Indirect effects of Suicide Crisis Syndrome (SCS) symptoms were found on the association of age, gender, and marital status with MH utilization and of age, marital status, and education with SP Utilization.
Conclusions
Specific demographic populations demonstrate greater interest in mental health care during the COVID-19 pandemic. These help-seeking patterns can be explained in part by an elevated level of SCS symptoms, suggesting greater levels of distress were driving expressed intention to utilize service referrals.
Novel fabricated chitosan supported bimetallic nanocomposites for paracetamol removal from homogeneous solutions and heterogeneous mixtures with focused antibacterial studies
Authors: Priyadarshi Gautam V. , Raval Nirav P. , Barcelo Damia , Trivedi Mrugesh H.
Published: November 2023

Biopolymers infused with bimetallic nanoparticles exhibit a wide range of functionalities necessary for efficiently eliminating diverse water contaminants. However, the protracted production process requires further exploration. As such, this current study seeks to optimize microwave-assisted technique for the facile synthesis of cross-linked chitosan (CTS) supported bimetallic oxide nanoparticles, specifically zinc oxide (ZnO) and iron oxide (Fe3O4), denoted as CTS-TTP/Zn-Fe. The primary objective is to investigate the efficacy of these beads in the removal of Paracetamol (PCM) from single and complex water matrices while also assessing their antibacterial properties. Characterization includes chemical composition, surface structures, thermal stability, and magnetic properties. The experimental results demonstrated that CTS-TPP/Zn-Fe beads achieved a remarkable PCM removal efficiency of approximately 99%, with a Zn:Fe mole ratio of 1:1. The experimental data showed good applicability with Freundlich isotherm and chemisorption-supported rate models (R2>0.9). To evaluate the long-term viability and practicality of these beads, three crucial field applicability tests were conducted. These encompassed competition studies with other pharmaceuticals, desorption investigations for repeated use, and efficiency evaluations in an ionic solution. Collectively, this research provides a comprehensive understanding, spanning from material design to practical applications, with potential relevance for large-scale wastewater treatment when coupled with appropriate flux control measures.
Modulating Leidenfrost-like Prompt Jumping of Sessile Droplets on Microstructured Surfaces
Authors: Cheng Jiangtao , Huang Wenge , Zhao Lei , Li Yang , He Xukun , Collier C. , Zheng Zheng , Liu Jiansheng
Published: November 2023

The Leidenfrost effect, namely the levitation and hovering of liquid drops on hot solid surfaces, generally requires a sufficiently high substrate temperature to activate the intense liquid vaporization. Here we report the agile modulations of Leidenfrost-like prompt jumping of sessile water microdroplets on micropillared surfaces at a remarkably mitigated temperature. Compared to traditional Leidenfrost effect occurring above 230 °C, the fin-array-like micropillars enables Wenzel-state water microdroplets to levitate and jump off within 1.33 ms at an unprecedently low temperature of 130 °C by triggering the inertia-controlled growth of individual vapor bubbles at the droplet base. We demonstrate that droplet jumping, resulting from the momentum interactions between the expanding vapor bubble and the droplet, can be deftly modulated by simply tailoring the thermal boundary layer thickness via pillar heights, which acts to regulate the bubble expansion between the inertia-controlled mode and the heat-transfer-limited mode. Intriguingly, the two bubble growth modes give rise to distinct droplet jumping behaviors characterized by constant velocity and constant energy schemes, respectively. This heating strategy allows the facile purging of wetting liquid drops on rough or structured surfaces in a controlled manner, inspiring promising applications in rapid removal of fouling even settled in surface cavities.
Structure and electrocatalytical properties of electrodeposited M-Ir (M=Co, Ni) bimetallic alloy catalysts with low Ir loading obtained on copper foams for hydrogen evolution reaction
Authors: Zhou Yicheng , Ju Liu , Yang Yang , wu wangping
Published: November 2023

M-iridium (M = cobalt (Co), nickel (Ni)) bimetallic alloy catalysts with low iridium (Ir) loading of 0.3 ~ 2.0 mg·cm− 2 were prepared on copper foam (CF) supports by electrodeposition. The top surface of as-deposited M-Ir catalysts was mainly composed of metallic state and oxides states, such as metallic Ir, Ni(OH)2 or Co(OH)2, Co(Ir) and Ni(Ir) solid solution, Ir oxides. M-Ir catalysts with low Ir loading exhibited excellent catalytic performance. Ni67.4Ir32.6/CF catalyst with low Ir loading of 2.0 mg·cm− 2 achieved a current density of 10 mA·cm2 at an overpotential of 52 mV and a Tafel slope of 36 mV·dec− 1. Co64.2Ir35.8/CF catalyst with low Ir loading of 0.7 mg·cm− 2 was uniformly scattered with small ellipsoidal particles, looking like fine fluff, requiring an overpotential of 51 mV for hydrogen evolution reaction to reach a current density of 10 mA·cm− 2, having a Tafel slope of 38 mV·dec− 1. After long-term hydrogen evolution testing, M-Ir/CF catalysts exhibited excellent electrocatalytic stability for water splitting in alkaline solution.
Enlarged Perivascular Space in the Basal Ganglia Associated with Coexisting Intracerebral Hemorrhage in Patients with Recent Small Subcortical
Authors: wu zhengrong , Zong Ce , Zhang Ke , Liu Hongbing , Wang Yanhong , Xu Yuming , Gao Yuan
Published: November 2023

Background: Intracerebral hemorrhage (ICH) is a common vascular disease associated with small blood vessels, and it is not uncommon for individuals with cerebral small vessel disease (CSVD) to develop ICH. Studies have shown that the number and location of cerebral microbleeds (CMBs) are associated with ICH in individuals with cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL). However, the prevalence of ICH and its related risk factors in individuals with recent subcortical small infarct (RSSI) is still unclear.
Methods: This study included 361 patients with complete MRI data. ICH was defined based on the medical history of cerebral hemorrhage and brain MRI and SWI scans, which are sensitive for detecting both old and recent hemorrhages. The location and quantity of enlarged perivascular spaces (EPVS) were assessed using a visual scoring scale. Additionally, evaluation was performed for CMBs,cerebral white matter hyperintensities (HWMHs)and lacunar infarctions(LI). The correlation between ICH and EPVS was analyzed using multivariate logistic regression analysis.
Results: The study ultimately included 361 patients with RSSI in the lenticulostriate arterial region, with a mean age of 59.70±12.11 years. Among them, 243 (67.3%) were male, and 34 (9.4%) had concomitant ICH. In univariate analysis, the proportion of patients with a history of cerebral infarction was higher in the ICH group, platelet count was lower, and there were higher proportions of basal ganglia perivascular spaces (BG-HEPVS),CMBs,deep/mixed CMBs,HCMBs (high CMBs),high white matter hyperintensities (HWMHs), and LI. The median CSVD total burden score was also higher in the ICH group.Multivariable logistic regression analysis showed that in Model 1, BG-HEPVS(OR, 4.12; 95% CI, 1.34-12.70; P=0.014) and deep/mixed CMBs (OR, 5.62; 95% CI, 1.50-21.03; P=0.010) were significantly associated with ICH. In Model 2, the total burden of CSVD (OR, 2.26; 95% CI, 1.54-3.33; P<0.001) was significantly associated with ICH.
Conclusion: In the population with RSSI, BG-EPVS, deep/mixed CMBs and the total burden of CSVD were significantly associated with ICH. The strength of this association and the ability to identify the risk of bleeding in clinical practice may be useful for the safety of future antithrombotic treatments.
Evaluating the AllplexTM GI-Bacteria (I) Assay Efficiency to Assess the Diversity of Bacterial Pathogens among Young Children with Diarrhea in Burundi
Authors: Ihorimbere Theogene , Nzoyikorera Néhémie , Gehre Florian , Sangare Modibo , Kezakarayagwa Eric , Kabatesi Francine , Nduwimana Cassien , Ndihokubwayo Armstrong , Nibogora Celestin , Affara Muna , Molina Andrea , Idris Hakim Lagu , Achol Emmanuel , Nyandwi Joseph
Published: November 2023

Background
Diarrheal infections are caused by many enteric pathogens that infect children as early as birth. Molecular assays are uncommon in routine diagnosis of gastrointestinal bacterial pathogens in Burundi. This study aimed to characterize the diversity of bacterial pathogens among young children with diarrhea in selected healthcare settings and to demonstrate the efficiency of the AllplexTM GI-Bacteria (I) assay over the conventional culture.
Methods
We conducted a cross-sectional study in seven (7) selected health districts in Burundi. We used a questionnaire to collect sociodemographic information for children ≥ 5 years old whose parents consented to participate. We collected stool samples for both conventional cultures (gold standard) and AllplexTM GI assay. We plated the stool samples shortly after collection and we kept the samples in the fridge between 4–8 ℃ for less than 24 hours before we processed them with the AllplexTM GI kit. We determined the association between sociodemographic factors and carriage of genes using a chi-square test for independence (Pearson's chi-square test or chi-square test of association). A p-value < 0.05 was considered statistically significant.
Results
17.6% (18/102) of stool specimens processed with conventional methods were positive for Shigella spp. (13.7%) and Salmonella spp (3.9%). With Allplex GI, 79.4% (81/102) of stool samples were positive for different genes; sh/ei genes were detected in 40.2% (40/102) of the stool samples. An association of two genes namely sh/ei and sal or sh/ei and vib was found. AllplexTM GI-Bacteria(I) PCR Assay had a sensitivity of 94.4% and a negative predictive value (NPV) of 95.2%. There was no association between place of residence, fingernail status, level of education, and being positive for sh/ei, sal, sh/ei and sal and sh/ei and vib.
Conclusion
We characterized bacterial pathogens in the stool samples and we demonstrated the efficiency of the AllplexTM GI assay over conventional methods. The data could further inform the decision-makers to institute effective prevention and control measures to tackle infectious diarrhea.
Investigating Antialzheimer Potential of Biogenic Compounds From Zinc 15 Database As NMDA Antagonist: A Insilico Approach.
Authors: Chaudhari Somdatta Y. , Shinde Asavari R. , Madar Pratiksha R. , Shingan Aarti Vijayakumar , Bairagi Shriram Hiradas , Dhage Azad Nabilal , Patel Pinkal , Rathod Vivek Digamber , Jadhav Shailaja B. , Chaudhari Pravin D.
Published: November 2023

Alzheimer's disease is an unavoidable neurological disorder in which memory loss, cognitive decline, and eventual dementia are brought on by the death of brain cells. There is no recognized treatment for Alzheimer's illness. By the year 2050, it is expected that the global population will witness approximately 100 million cases of Alzheimer’s disease (AD). Despite recognizing AD as a formidable illness for over a century, no effective cure has been discovered thus far. Synaptic dysfunction could result from disturbed synaptic calcium handling caused by excessive activation of glutamate receptors, particularly the N-methyl-D-aspartate receptors (NMDARs). Glutamate serves as the main excitatory neurotransmitter in the brain, acting on both ionotropic and metabotropic glutamate receptors.
In recent years, a number of pharmacologically active substances that were derived from plants, animals, and microbes have shown promise in the treatment of AD by focusing on various pathogenic processes. Initially, we used virtual screening to assess natural product-like compounds against NMDA receptors. In this research study, we have screened a natural compound database derived from zinc15. The best candidate was then validated through molecular dynamics simulation (MDS). The results revealed that out of 4221 compounds tested, only 165 showed superior binding interactions compared to native ligands, making them inhibitors for protein. Further analysis using ADMET indicates favorable drug-like properties, particularly for CNS drug-likeness. The MDS results, including RMSD, RMSF, Rg, and residue interactions, indicated a strong and stable association between top molecules and target protein. This confirms that top molecules can effectively remain within the binding pockets of the target proteins, forming stable protein-ligand complexes.
Effect of Post-Transplant Diabetes Mellitus on Cardiovascular Events and Mortality: A single‐center retrospective cohort study
Authors: Ünlütürk Uğur , Yıldırım Tolga , Savaş Merve , Oğuz Seda Hanife , Fırlatan Büşra , Yüce Deniz , Karakaplan Nesrin Damla , Selimova Cemile , Yılmaz Rahmi , Erdem Yunus , Bayraktar Miyase
Published: November 2023

Purpose
This study aims to investigate the impact of post-transplant diabetes mellitus (PTDM) on cardiovascular events, graft survival, and mortality and to determine the risk factors involved in developing PTDM.
Methods
A total of 703 patients who underwent kidney transplantation were included in the study. The total sample was subdivided into three groups: (i) patients with PTDM; (ii) patients who had diabetes before the transplantation (DM); and (iii) patients without diabetes (NoDM). The data on graft failure, cardiovascular events, all-cause mortality, and the potential risk factors that play a role in developing PTDM were recorded and analyzed.
Results
The patients were followed for a median of 80 (6-300) months after transplantation. Out of all patients, 41 (5.8%) had DM before transplantation, and 101 (14.4%) developed PTDM. Recipient BMI, post-transplant fasting plasma glucose, and hepatitis C seropositivity were independent risk factors for PTDM development. The incidence of cardiovascular events was 6.1% in the NoDM group, 14.9% in the PTDM group, and 29.3% in the DM group (p < 0.001). In PTDM patients, hepatitis C seropositivity and the recipient's age at transplant were independent predictors of a cardiovascular event. There were no significant differences between the groups regarding the risk of graft loss. PTDM had no significant effect on all-cause mortality. However, the survival rates of DM patients were significantly reduced compared to those with NoDM or PTDM.
Conclusions
PTDM had no impact on patient survival. Hepatitis C seropositivity and recipient age at transplant predicted cardiovascular events in PTDM patients.
Assertion connotation of Carcinoembryonic Antigen a complex glycoprotein in colorectal cancer
Authors: Younis Mohd
Published: November 2023

Background: Carcinoembryonic antigen (CEA) is a large glycoprotein that regulates intercellular adhesion and is most widely used molecular biomarker for the diagnosis and evaluation of CRC globally.
Aim: In the present study we aimed to evaluate CEA serum expression patterns in CRC patients among the South Indian population.
Method:A total of 130 individuals with colorectal cancer (n=65) and healthy controls (n=65) were evaluated by analyzing the concerted and individual effects of the tumor biomarker carcinoembryonic antigen by standard protocol.
Result: The overall CEA levels were significantly (p-value <0.001) higher in CRC patients i.e., 9.30±3.20 ng/ml than in the controls (2.83±0.57ng/ml). The mean level of CEA in Dukes stages A (7.36±1.98ng/ml) was significantly different from Duke stage D (11.75±2.05ng/ml). Exploring the smoking and alcohol found significant (<0.0001) associated with the risk of CRC OR: 5.83, 95% CI: [2.63-12.93] & OR: 6.36, 95% CI: [2.93-13.79] respectively. The experimental subjects with smoke and alcohol consumption have significantly increased mean levels of CEA (9.42±3.27), and (9.21±3.26) respectively.
Conclusion: We conclude that overall CEA levels were high in CRC patients in contrast to healthy controls. Also, smoking and alcohol consumption significantly increases the risk of CRC.
Integrated Bioinformatic Analysis to Decipher the Contribution of Bisphenol-A in Cervical Cancer
Authors: G Nadeem Khan , Adiga Divya , Rai Padmalatha S , Kabekkodu Shama Prasada
Published: November 2023

Bisphenol A (BPA) is a synthetic chemical widely used as a monomer for producing polycarbonate plastics. The present investigation employed an in-silico approach to identify BPA-responsive genes and comprehend the biological functions affected using in vitro studies. A Comparative Toxicogenomics Database search identified 29 BPA-responsive genes in cervical cancer (CC). Twenty-nine genes were screened using published datasets, and thirteen of those showed differential expression between normal and CC samples. Protein-Protein Interaction Networks (PPIN) analysis identified BIRC5, CASP8, CCND1, EGFR, FGFR3, MTOR, VEGFA, DOC2B, WNT5A, and YY1 as hub genes. KM-based survival analysis identified that CCND, EGFR, VEGFA, FGFR3, DOC2B, and YY1 might affect CC patient survival. SiHa and CaSki cell proliferation, migration, and invasion were all considerably accelerated by BPA exposure. Changes in cell morphology, remodelling of the actin cytoskeleton, increased number and length of filopodia, elevated intracellular reactive oxygen species and calcium, and lipid droplet accumulation were noted upon BPA exposure. BPA treatment upregulated the expression of epithelial to mesenchymal transition pathway members and enhanced the nuclear translocation of CTNNB1. We showed that the enhanced migration and nuclear translocation of CTNNB1 upon BPA exposure is a calcium-dependent process. The present study identified potential BPA-responsive genes and provided novel insights into the biological effects and mechanisms affected by BPA in CC. Our study raises concern over the use of BPA.
In situ hybridization analysis of the expression of class I and II odorant receptors in the olfactory organ of the African spurred tortoise (Centrochelys sulcata)
Authors: Nakamuta Shoko , Zhang Zicong , Nikaido Masato , Noda Hideki , Yokoyama Takuya , Yamamoto Yoshio , Nakamuta Nobuaki
Published: November 2023

In this study, in situ hybridization was used to determine the localization of odorant receptor (OR)-expressing cells in the olfactory organ of the African spurred tortoise (Centrochelys sulcata). The olfactory organ of turtles generally consists of an upper chamber epithelium (UCE) containing glands and a lower chamber epithelium (LCE) devoid of glands. The UCE is considered an air-nose that is receptive to airborne odorants, whereas the LCE is considered a water-nose that is receptive to waterborne odorants. The ORs are a family of olfactory receptors categorized into classes I and II based on their sequence similarities. Class I ORs are thought to be receptive to water-soluble odorants, and class II ORs to volatile odorants. However, where these receptors are expressed within the olfactory organ in turtles remains unclear. This study was performed to analyze the distribution of OR-expressing cells in the olfactory organ of C. sulcata. Class II ORs were revealed to be expressed in the UCE, implying that the UCE of C. sulcata functions as an air-nose. In contrast, class I ORs were expressed in both the UCE and LCE, implying that both of these epithelia function as a water-nose in the olfactory organ of C. sulcata. Furthermore, double-labeling experiments indicated that class I ORs are not co-expressed with the transcription factor B-cell lymphoma/leukemia 11b (Bcl11b), implying that the regulatory mechanism by which Bcl11b suppresses class I OR expression is conserved in C. sulcata. Further studies are needed to clarify the functional significance of the expression of class I ORs in the entire olfactory organ and to determine whether this is a trait shared among terrestrial turtles or found only in one species of tortoise that inhabits arid regions.
Ultrasound-guided versus conventional lung recruitment manoeuvres in thoracic surgery: A randomised controlled study
Authors: Wu Lei , Yang Li , Yang Yanyan , Wu Xin , Zhang Jun
Published: November 2023

Purpose: Although lung recruitment manoeuvres (RMs) during mechanical ventilation may reduce atelectasis, the optimal recruitment strategy for patients undergoing thoracic surgery remains unknown. Our study was to investigate whether ultrasound-guided lung RMs is superior to conventional RMs in reducing incidence of perioperative atelectasis during thoracic surgery with one-lung ventilation.
Methods: We conducted a randomised controlled trial from August 2022 to September 2022. Sixty patients scheduled for video-assisted thoracoscopic surgery (VATS) under general anaesthesia were enrolled. Subjects were randomly divided into the ultrasound-guided RMs group (manual inflation guided by lung ultrasound) or conventional RMs group (manual inflation with 30 cmH2O pressure). Ultrasound were performed at three predefined time points (1 min after anaesthetic induction; after RMs at the end of surgery; before discharge from postanesthesia care unit [PACU]). The primary outcome was lung ultrasound score before discharge from the PACU after extubation.
Results: In the early postoperative period, lung aeration deteriorated in both groups even after lung RMs. However, ultrasound-guided lung RMs had significantly lower lung ultrasound scores when compared with conventional RMs in bilateral lungs (2.0 [0.8–4.0] vs. 8.0 [3.8–10.3], P < 0.01) at the end of surgery, which remained before patients discharged from the PACU. Accordingly, the lower incidence of atelectasis was found in ultrasound-guided RMs group than in conventional RMs group (7% vs. 53%; P < 0.01) at the end of surgery.
Conclusion: Ultrasound-guided RMs is superior to conventional RMs in improving lung aeration and reducing lung atelectasis at early postoperative period in patients undergoing VATS.
Trial registration: The study protocol was approved by the Institutional Review Board of the Fudan University Shanghai Cancer Center (No.220825810; date of approval: August 5, 2022) and registered on Chinese Clinical Trial Registry (number: ChiCTR2200062761).
Single-Cell Transcriptomics of Glioblastoma Reveals Pericytes Contributing to Blood–Brain Tumor Barrier and Tumor Progression
Authors: Li Yuzhe , Wu Changwu , Long Xinmiao , Wang Xiangyu , Gao Wei , Deng Kun , Xie Bo , Zhang Sen , Wu Minghua , Qing Liu
Published: November 2023

Background:Pericytes compose blood–brain barrier (BBB) and may affects the blood-brain tumor barrier function (BBTB) in glioblastoma (GBM), which eventually affect chemotherapy efficiency and tumor progression of the disease. However, the expression signatures and detailed effect of these pericyte are still unclear. We aimed to identify these pericytes affecting BBTB in GBM, and to explore their clinical effect and underlying functions.
Methods: Pericytes in GBM were identified from single-cell RNA sequencing (scRNA-seq) da ta from nine GBM samples by hallmarks and expression signatures of pericytes from previous researches, and cell cluster functional enrichments were also used in identification. CellPhoneDB were used to explore interactions between target pericyte and other cells. One in-house glioma clinical cohort was collected and included for survival analysis. CGGA, REMBRANDT, and GSE16011 GBM cohorts were used to develop and validate a tumor pericyte risk score (TPRS) for prognosis prediction.
Results: This study demonstrated that a group of pericytes concerning BBTB function exist in GBM, and PTH1R may be a specific biomarker for them. The pericytes of BBTB function interact with other cell in GBM mainly through extracellular matrix (ECM)-integrin signaling pathways. Also, when comparing with normal pericytes, pericytes in GBM has up-regulation of several ECM gene networks (THY1, COL3A1, COL4A1, TIMP1, FN1 etc.) related to poor prognosis and basement membrane formation. TPRS based on those differentially expressed genes (DEGs) has significant predictive value in GBM patients.
Conclusions:Pericytes of BBTB function in GBM exist and PTH1R potentially serving as a hallmark for them. These cells have close functional relationships with BBTB and ECM-integrin signing pathways, and may have predictive value for GBM patients.
Determining the Toxic Elements in Cannabinoid and Opioid Drugs and Their Impact on Addicts' Health
Authors: UNAR AHSANULLAH , Sahito Oan , Afridi Hassan
Published: November 2023

Drug addiction is associated with significant health risks, including cardiovascular complications, cancer, and mental disorders. Illicit drugs, such as cannabinoids and opioids, including prescription medications, are widely consumed and have profound health consequences. Understanding the health effects of the toxic elements in these substances is critical for overdose prevention and effective recovery strategies. This study aimed to determine the toxic elements, including arsenic (As), cadmium (Cd), mercury (Hg), and nickel (Ni), in cannabinoid and opioid drugs and in biological samples (blood, scalp hair, and serum) from 311 male drug abuse patients aged 15 to 60 years with a history of drug abuse. The participants were categorized into three age groups. The comparative analysis involved 113 reference subjects of the same age groups. The sample preparation employed microwave-assisted acid digestion, and the toxic elements were quantified using atomic absorption spectrophotometry. Accuracy was ensured using certified reference materials for hair, blood, and serum. Drug-addicted subjects had significantly higher concentrations of toxic elements (arsenic, cadmium, mercury, and nickel) in biological samples than referent subjects (p > 0.001). Elevated levels of these toxic elements may increase susceptibility to infections, possibly due to malnutrition, drug-related effects, and additional contaminants. These findings necessitate further studies to explore the long-term health outcomes, potential treatment options, and broader socioeconomic impacts of substance abuse. This study serves as a baseline for future research in this critical public health field.
A Study on the Effect of Entrepreneurship Education on College Students' Entrepreneurial Intention - Mediated by Entrepreneurial Attitudes and Moderated by Entrepreneurial Policies
Authors: Yifan Nie , Ai Jiang , Yu Liu , Yunlandi Wu
Published: November 2023

As college students begin to become the force of mass entrepreneurship in China, effectively enhancing college students' entrepreneurial intention has become a key issue that needs to be solved urgently. This study takes entrepreneurship education as an indicator of the influence of entrepreneurial intention, and examines how different types of entrepreneurship education (theoretical, practical, and ambient education) act on entrepreneurial intention through entrepreneurial attitudes (endogenous and exogenous attitudes). By examining the data from 427 valid questionnaires from nine universities in Beijing, this study found that: entrepreneurship theory, practice, and atmosphere education have a significant positive impact on college students' entrepreneurial intention; entrepreneurship education significantly and positively influences college students' endogenous entrepreneurial attitude and exogenous entrepreneurial attitude; entrepreneurship education has a significant positive impact on college students' entrepreneurial intention through the two mediator variables of endogenous entrepreneurial attitudes, exogenous entrepreneurial attitudes entrepreneurship education has a significant positive effect on college students' entrepreneurial intention through the mediating variables of endogenous entrepreneurial attitude and exogenous entrepreneurial attitude; entrepreneurship education has a significant positive effect on college students' entrepreneurial intention through the mediating variables of endogenous entrepreneurial attitude and exogenous entrepreneurial attitude; entrepreneurship policy has a significant positive effect on college students' entrepreneurial intention through the mediating variables of entrepreneurial theory, practice, and atmosphere education.
The impact of metabolic syndrome on patient-reported outcome measures after primary total knee arthroplasty in osteoarthritis patients
Authors: Leng Yi , Gao Yuhang , Zhang Jianzeng , Zhang Yibin , Zhang Chengshuai , Qi Xin
Published: November 2023

Background
As the proportion of patients with metabolic syndrome (MetS) among those undergoing total knee arthroplasty (TKA) for osteoarthritis increases annually, the impact of MetS on patient-reported outcome measures (PROMs) after TKA has been the subject of conflicting research findings. Our goal is to determine the influence of MetS on PROMs during the first year after TKA and its associated trajectory of change.
Methods
We reviewed data from patients receiving their primary TKA,. Using the full-length X-ray of the patient's lower limb. We calculated the Metabolic Score for Insulin Resistance (MetS-IR) to reflect the severity of metabolic syndrome and used Maximally Selected Test Statistics to divide patients into two groups based on MetS-IR. We analyzed pre- and post-TKA OKS as PROMs and further analyzed OKS pain and function subscores.
Results
A total of 124 patients were included in the study. The selected cutoff value for MetS-IR was 43. Patients with MetS-IR < 43 were classified as Group 1 (n=79), while those with MetS-IR ≥ 43 were classified as Group 2 (n=45). Patients in Group 2 were younger (63.26±6.23, P<0.05), had poorer preoperative OKS scores (22 (17), P<0.05), more severe intra-articular deformities (JLCA: 6.01±2.99, MAD: 33.11±19.29, P<0.05), and higher levels of inflammation (CRP: 3.67 (2.15), P<0.05). Furthermore, CRP levels remained elevated at 1 month (7.19 (4.42)) and 3 months (5.24 (2.66)) postoperatively. Although postoperative OKS scores were lower in Group 2 than in Group 1, the improvement in OKS was higher in Group 2. Multiple regression analysis showed that MetS-IR was an independent predictor of OKS (β= -0.45, -0.19, -0.17, -0.24, p<0.05) and could predict preoperative, 3-month, 6-month, and 12-month OKS.
Conclusion
MetS-IR has a better predictive ability for early postoperative outcomes after TKA compared to the clinical classification of metabolic syndrome. Patients with high MetS-IR show greater improvement compared to those with low MetS-IR, however, their OKS levels remain relatively lower. MetS-IR is an independent predictive factor for OKS in OA patients before and after TKA, and a higher preoperative MetS-IR suggests lower postoperative PROMs. These findings may provide valuable insights for improved understanding and management of these patients.
Kinematic Descriptors of Arm Reaching Movement are Sensitive to Hemisphere-Specific Immediate Neuromodulatory Effects of Transcranial Direct Current Stimulation Post Stroke
Authors: Lowenthal-Raz Justine , Liebermann Dario G. , Friedman Jason , Soroker Nachum
Published: November 2023

Transcranial direct current stimulation (tDCS) exerts beneficial effects on motor recovery after stroke, presumably by enhancement of adaptive neural plasticity. However, patients with extensive damage may experience null or deleterious effects with the predominant application mode of anodal (excitatory) stimulation of the damaged hemisphere. In such cases, excitatory stimulation of the non-damaged hemisphere might be considered. Here we asked whether tDCS exerts a measurable effect on movement quality of the hemiparetic upper limb, following just a single treatment session. Such effect may inform on the hemisphere that should be excited. Stroke patients and healthy controls were assessed before and after anodal, cathodal or sham tDCS, provided during a single session of reaching training (repeated point-to-point hand movement on an electronic tablet). Group comparisons of endpoint kinematics - number of peaks in the speed profile (NoP; smoothness), hand-path deviations from the straight line (SLD; accuracy) and movement time (MT; speed) – disclosed greater NoP, larger SLD and longer MT in the stroke group. NoP and MT revealed an advantage for anodal compared to sham stimulation of the lesioned hemisphere. NoP and MT improvements under anodal stimulation of the non-lesioned hemisphere correlated with severity of hemiparesis. Damage to specific cortical regions and white-matter tracts was associated with lower kinematic gains from tDCS.
An efficient method for the selective isolation of feline herpesvirus 1 (FHV-1) in feline calicivirus (FCV) coinfected specimens
Authors: Zheng Huanqin , Wang Baoyan , YUE Hong , Yu Xin , Liu Yang , Yu Jiayu , Zhang Jianlong , Han Kexue , Han Yinuo , Su Hanfeng , Zhu Hongwei , Zhang Xingxiao
Published: November 2023

Background
Feline herpesvirus-1 (FHV-1) and feline calicivirus (FCV) are the most common viral pathogens of feline respiratory disease and are highly prevalent in cats worldwide. Coinfection with these viruses is frequent in cats with feline respiratory disease complex (FRDC). It is difficult to isolate pure FHV-1 by conventional laboratory cell culture methods from specimens with FRDC, which brings great trouble to the epidemiological investigation of FHV-1 virus.
Methods
FCV polyclonal antibodies were obtained by immunizing rabbits, and the coinfected specimens were neutralized with FCV polyclonal antibodies. Then, virus isolation was performed. After several rounds of neutralization, FHV-1 was finally obtained.
Results
The FCV polyclonal antibody was successfully obtained with neutralizing activities of 1:128, 1:537, and 1:91. After antibody neutralization, the FHV-1 virus was successfully isolated from the coinfected cell culture suspension and confirmed by immunofluorescence and QRT-PCR.
Conclusion
In this study, all FHV-1 viruses present in the coinfection samples were isolated, without any cross-contamination. This method is also theoretically suitable for the isolation and purification of other FCV coinfections or contaminating disease substances.
Erster Nachweis von Hypnum vaucheri LESQ. in Sachsen-Anhalt
Authors: Müller Frank
Published: November 2023

Der Erstfund für Sachsen-Anhalt von Hypnum vaucheri im Unstruttal bei Steigra wird mitgeteilt.
Sprossende Hauswurz (Sempervivum globiferum L. subsp. globiferum) in Mitteldeutschland
Authors: Nußmann Thomas , John Heino
Published: November 2023

Dieser Beitrag dokumentiert das Vorkommen von Sempervivum globiferum subsp. globiferum in Mitteldeutschland (Sachsen, Sachsen-Anhalt und Thüringen) und versucht, Schlussfolgerungen aus dem vorliegenden Verbreitungsmuster zu ziehen. In Mitteldeutschland finden sich neben dem häufig angepflanzten Sempervivum tectorum auch traditionelle Anpflanzungen von Sempervivum globiferum, insbesondere auf Mauern und Toranlagen alter Gehöfte in den Ortskernen. Auffällige Häufungen der Verwendung von S. globiferum anstelle von S. tectorum oder der gemeinsamen Verwendung auf Mauern und Torpfeilern wurden in den Dörfern der Täler der Elster von Sachsen, Sachsen-Anhalt und Thüringen, der Unstrut bis zum Südharz und im Gebiet der Ilm in Thüringen festgestellt. Tatsächlich gibt es eine gute Übereinstimmung zwischen den Nachweisen von S. globiferum auf Mauerkronen und Torpfeilern und Nachweisen früher friedlicher Kontakte von aus dem Osten zugewanderten Volksgruppen (wohl vorwiegend Slawen) mit germanischen Volksgruppen. Verwilderungen von S. globiferum findet man nie im Tiefland links der Elbe. Dagegen können sich in montanen Bereichen nach Verwilderungen sehr stabile Populationen an Felsformationen entwickeln, dieeinen natürlichen Eindruck erwecken.
Neufunde und Bestätigungen bemerkenswerter Pflanzenarten Sachsen-Anhalts im Jahr 2011
Authors: Ziesche Heinz
Published: November 2023

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Moosvegetation des Herrscherbergs und des Bocks bei Nebra (Burgenlandkreis, Sachsen-Anhalt)
Authors: Marstaller Rolf
Published: November 2023

Im Gebiet des Herrscherbergs und des Bocks bei Nebra, im südlichen Sachsen-Anhalt gele-gen, wurden auf Sandsteinblöcken, Mineralböden, Borke lebender Gehölze und morschem Holz die Moosgesellschaften und die Moosflora erfasst. Charakteristische Gesellschaften sind azidophytische Assoziationen der Verbände Grimmion commutatae, Isothecion myosuroidisund Dicranellion heteromallae, basiphytische Assoziationen der Verbände Grimmion tergestinae, Grimaldion fragrantis, Neckerion complanatae und Fissidention gracilifolii, epiphytische Gesellschaften der Verbände Dicrano scoparii-Hypnion filiformis, Ulotion crispae und Syntrichion laevipilae sowie auf morschem Holz Gesellschaften des Verbandes Bryo-Brachythecion. Alle Gesellschaften sind durch zahlreiche Aufnahmen in 22 Tabellen dargestellt. Insgesamt konnten 42 Moosgesellschaften und 155 Moosarten (9 Lebermoose, 146 Laubmoose) nachgewiesen werden.
Ergebnisse der Kartierung der Armleuchteralgen im Süden von Sachsen-Anhalt
Authors: Korsch Heiko
Published: November 2023

Nach einer kurzen Einführung zur Ökologie und zur Erforschung der Armleuchteralgen werden das Untersuchungsgebiet im Südteil von Sachsen-Anhalt umgrenzt und die Methoden der Erfassung der Armleuchteralgen erläutert. Bei den Untersuchungen konnten 15 Characeen-Arten nachgewiesen werden. Die Häufigkeiten der einzelnen Sippen werden in einer Tabelle und die Artenzahlen in einer Karte dargestellt. Für die seltenen Arten werden die konkreten Fundorte genannt. Darauf folgen Hinweise für eine Aktualisierung der Roten Liste und ein Aufruf zur Erfassung sowie Tipps zum Sammeln von Armleuchteralgen.
Wiederfund von Najas marina L. ssp. marina im Schollener See (Elbe-Havel-Winkel, Sachsen-Anhalt)
Authors: Knösche Rüdiger
Published: November 2023

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Rote Listen der Farn- und Blütenpflanzen Sachsen-Anhalts – Expertenvoten mit begrenzter statistischer Auswertbarkeit relevanter Umweltfaktoren
Authors: Frank Dieter
Published: November 2023

Die Einstufungen der Gefährdungsgrade in den vier Roten Listen gefährdeter Pflanzenarten für das Bundesland Sachsen-Anhalt aus den Jahren 1978, 1992, 2004 und 2020 werden vergleichend interpretiert. Für Arten, die einen positiven oder negativen Trend der Gefährdungseinstufung aufweisen, werden die biologisch-ökologischen Indikatormerkmale ‚Nährstoffzahl‘, ‚Feuchtezahl‘ und ‚Strategietyp‘ vergleichend interpretiert. In der Regel ist die Gefährdung einzelner Arten durch eine Kopplung mehrerer Umweltfaktoren zu erklären. Eine individuelle Interpretation von Gefährdungs-Trends wird empfohlen. Besonders relevante Gefährdungsursachen sind Eutrophierung, Uniformierung der Landnutzung, Gewässerverschmutzung und Verlust von Sonderstandorten.
Bemerkenswerte Funde im südlichen Sachsen-Anhalt
Authors: John Heino , Stolle Jens
Published: November 2023

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Carl ALTEHAGE – sein Wirken für den Naturschutz in Mitteldeutschland
Authors: Hensel Gunnar
Published: November 2023

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Untersuchungen zur Verbreitung, Ökologie und genetischen Variation des Speierlings (Sorbus domestica L.) in Sachsen-Anhalt
Authors: Steffens Roland , Zander Matthias
Published: November 2023

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Zur Flora von Bitterfeld und Umgebung (8. Beitrag)
Authors: Wölfel Ulrich
Published: November 2023

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Leucanthemum ircutianum DC. und Leucanthemum vulgare LAM. in Sachsen-Anhalt
Authors: Scholz Christiane , Uhlemann Ingo
Published: November 2023

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Bestimmung von Weiden (Salix L.) und deren Hybriden in Sachsen-Anhalt
Authors: Jäger Urs G.
Published: November 2023

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Xerothermrasen-Gesellschaften des unteren Unstruttales und einige ökologische Gründe für ihre Verteilung im Raum
Authors: Becker Thomas
Published: November 2023

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Juncus atratus KROCKER und Orobanche alsatica Kirschl., zwei bemerkenswerte Funde in Sachsen-Anhalt
Authors: Müller Jochen , Renker Carsten , Heinrichs Jochen
Published: November 2023

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Zur Flora des Meßtischblattes Sangerhausen (4533)
Authors: Kison Hans-Ulrich , Gerlach Albrecht , Hanelt Peter , Illig Werner , Schmidt Volker
Published: November 2023

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Flechtenflora zweier Naturschutzgebiete im Selketal
Authors: Kison Hans-Ulrich , Czarnota P. , Schiefelbein U. , Sipman H. J. M. , Stordeur R. , Westermann A.
Published: November 2023

Die Flechtenflora der Naturschutzgebiete ‚Selketal‘ (NSG 73) und ‚Oberes Selketal‘ (NSG 178) einschließlich lichenicoler Pilze wird vorgestellt. Im NSG 73 wurden insgesamt 178 Taxa festgestellt, davon sind 40 % in der Roten Liste Sachsen-Anhalts verzeichnet und knapp 60 % sind Waldarten. Für das NSG 178 konnten 289 Taxa festgestellt werden. 47 % der Arten sind hier in der Roten Liste verzeichnet und wiederum 60 % der Taxa sind Waldarten. 4 % der Arten haben eine Bindung an alte, naturnahe Wälder. Als Charakterarten der Felslebensräume beider NSG können Montanelia disjuncta, Psilolechia lucida (mit dem parasitischen Microcalicium arenarium), Tephromela grumosa und Umbilicaria hirsuta herausgestellt werden. Verbreitet sind weiterhin Lecanora rupicola subsp. rupicola und L. swartzii. Als Besonderheiten auf offenen Felsen sind zu nennen: Lasallia pustulata, Lecanora sulphurea, Pertusaria pseudocorallina, Protoparmelia badia, Ramalina capitata, R. pollinaria sowie in Kaltluftgebieten Ophioparma ventosa und Pertusaria corallina. Bemerkenswerte Epiphyten in Eichenwäldern sind Alyxoria varia, Bacidia rubella, Calicium adspersum, C. glaucellum, Pertusaria amara, P. flavida u. a. Arten alter Naturwälder in den Talauen sind Arthonia atra, A. radiata, Calicium viride, Chaenotheca brachypoda, Graphis scripta, Opegrapha vermicellifera und O. rufescens. Chrysothrix candelaris mit dem wohl einzigen aktuellen Vorkommen im Harz findet sich an Esche. Trockene Fels-Heiden weisen auf: Cetraria aculeata, Cladonia cervicornis, C. foliacea, C. mitis, C. verticillata sowie Dibaeis baeomyces. Neu für das Gebiet sind u. a. nachgewiesen Anisomerdium polypori, Peltigera extenuata, Rinodina aspersa, Ropalospora viridis und Usnea substerilis.
Zur Flora des Wippertales in der Umgebung von Wippra (MTB 4433)
Authors: Hoch Armin , Frank Dieter , Herdam Hagen , Illig Werner , Kison Hans-Ulrich
Published: November 2023

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Bibliographie botanischer Literatur über Sachsen-Anhalt 2012/2013
Authors: Krumbiegel Anselm
Published: November 2023

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Bibliographie botanischer Literatur über Sachsen-Anhalt 2005/2006
Authors: Krumbiegel Anselm
Published: November 2023

Die im Vorjahr begonnene Bibliographie zur botanischen Literatur über Sachsen-Anhalt wird mit Nachträgen zu 2004 und 2005 sowie den bisher zugänglichen Publikationen von 2006 fortgesetzt.
Bibliographie botanischer Literatur über Sachsen-Anhalt 2004 und 2005
Authors: Krumbiegel Anselm
Published: November 2023

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Wasser- und Sumpfpflanzen-Funde im Elb-Havel-Winkel (Biosphärenreservat „Flusslandschaft Elbe“, Sachsen-Anhalt, Landkreis Stendal) II. Neufunde, Wiederfunde und Nachträge
Authors: Täuscher Lothar , Paproth Rolf
Published: November 2023

Nach einer ersten Zusammenstellung wichtiger Funde von Wasser- und Sumpfpflanzen im Elb-Havel-Winkel (TÄUSCHER 1999) sollen mit dieser Mitteilung Neufunde, Wiederfunde und Nachträge dokumentiert werden.
Phytoflagellaten-Studien im Naturschutzgebiet „Aland-Elbe-Niederung“ mit Erstfunden für Sachsen-Anhalt
Authors: Kusber Wolf-Henning
Published: November 2023

Von dreizehn Phytoflagellaten, davon zehn Euglenophyta, die aus der „Hohe Garbe“ im Naturschutzgebiet „Aland-Elbe-Niederung“ beschrieben und dokumentiert werden, sind elf Arten neu für Sachsen-Anhalt. Phacus warszewiczii wird in vorliegender Publikation erstmals nach Funden aus Deutschland abgebildet.
Moosvegetation in der Umgebung von Zscheiplitz bei Freyburg/Unstrut (Burgenlandkreis, Sachsen-Anhalt)
Authors: Marstaller Rolf
Published: November 2023

Ziel der vorliegenden Arbeit ist die Vorstellung einer Übersicht über die Moose und Moosgesellschaften der Kalkrasen sowie der angrenzenden Vegetationstypen im Muschelkalkgebiet des unteren Unstruttales in der Umgebung von Zscheiplitz zwischen Laucha und Freyburg/Unstrut. Bedeutungsvoll sind in einem Kalksteinbruch, in verschiedenen Trocken- und Halbtrockenrasen sowie Xerothermgebüschen die epilithischen, photophytischen Assoziationen Orthotricho anomali-Grimmietum pulvinatae, Grimmietum orbicularis, das seltene Grimmietum tergestinae, die sciophytischen Gesellschaften Homomallietum incurvati und die Homalothecium sericeum-Gesellschaft. Auf Kalkböden gedeihen häufig das Tortelletum inclinatae, vereinzelt das Barbuletum convolutae, in den Fugen der Kalkbänke das Weissietum crispatae und Aloinetum rigidae mit Aloina obliquifolia. Auf der Borke lebender Laubgehölze wachsen vereinzelt das Orthotrichetum fallacis, Syntrichio latifoliae-Leskeetum polycarpae, lokal das Ulotetum crispae, Syntrichietum pulvinatae und Orthotrichetum pallentis. Alle Gesellschaften sind durch zahlreiche Vegetationsaufnahmen in 11 Tabellen dargestellt. Insgesamt wurden 21 Moosgesellschaften und 104 Moosarten (7 Lebermoose, 97 Laubmoose) nachgewiesen. Die Diskussion betrifft die bryogeographische Situation in der Umgebung von Zscheiplitz sowie die Lebensformen, Lebensstrategien und Reproduktionsstrategien der Moose und Moosgesellschaften.
Pioniervegetation auf einer altmärkischen Waldbrandfläche unweit von Salzwedel
Authors: Brennenstuhl Günter
Published: November 2023

Die Besiedlung einer Brandfläche in einer altmärkischen Kiefernkultur im Stangenholzalter mit Spermatophyten wurde über einen Zeitraum von sechs Vegetationsperioden verfolgt. Über die beobachteten Pionierund Folgestadien wird berichtet, wobei auf einige Gemeinsamkeiten bei der Erstbesiedlung von Brand- und Schlagflächen eingegangen wird.