bluuebunny/crossref_metadata_2025 huggingface dataset:
Purification of 3 monomeric monocot mannose-binding lectins and their evaluation for antipoxviral activity: potential applications in multiple viral diseases caused by enveloped viruses
Authors: Kaur Amandeep , Kamboj Sukhdev Singh , Singh Jatinder , Singh Rajinder , Abrahams Melissa , Kotwal Girish J. , Saxena A.K.
Published: May 2007

Three monomeric monocot lectins from Zephyranthes carinata, Zephyranthes candida, and Gloriosa superba with carbohydrate specificity towards mannose derivatives and (or) oligomannose have been isolated and purified from their storage tissues. The lectins were purified by anion-exchange chromatography on DEAE–Sephacyl and by gel filtration chromatography on Biogel P-200 followed by high-performance liquid chromatography. The purified lectins, Z. carinata, Z. candida, and G. superba had molecular masses of 12, 11.5, and 12.5 kDa, respectively, as determined by gel filtration and SDS–PAGE, indicating that they are monomers. In a hapten inhibition assay, methyl-α-d-mannopyranoside inhibited agglutination of both Z. candida and Z. carinata; the latter was also inhibited by Man(α1-2)Man and Man(α1-3)Man. Gloriosa superba showed inhibition only with Man(α1-4)Man of all of the sugars and glycoproteins tested. All purified lectins agglutinated red blood cells from rabbit, whereas G. superba was also reactive towards erythrocytes from guinea pig. All of the lectins were nonglycosylated and did not require metal ions for their activity. They were labile above 60 °C and were affected by denaturing agents such as urea, thiourea, and guanidine–HCl. The lectins were virtually nonmitogenic, like other members of Amaryllidaceae and Liliaceae. Of the 3 lectins, G. superba was found to be highly toxic to the BSC-1 cell line (African green monkey kidney epithelial cells), while both of the Zephyranthes species showed significant in vitro inhibition of poxvirus replication in BSC-1 cells without any toxic effects to the cells. In addition, Z. candida also exhibited significant anticancer activity against SNB-78, a CNS human cancer cell line.
An antibody-conjugated internalizing quantum dot suitable for long-term live imaging of cells
Authors: Kaul Zeenia , Yaguchi Tomoko , Harada Jun I. , Ikeda Yutaka , Hirano Takashi , Chiura Hiroshi X. , Kaul Sunil C. , Wadhwa Renu
Published: May 2007

Quantum dots (QD) are fluorescent semiconductor nanocrystals that are emerging as superior alternatives to the conventional organic dyes used in biological applications. Although QDs offer several advantages over conventional fluorescent dyes, including greater photostability and a wider range of excitation and (or) emission wavelengths, their toxicity has been an issue in its wider use as an analytic, diagnostic and therapeutic tool. We prepared a conjugate QD with an internalizing antibody and demonstrated that the QD–antibody conjugate is efficiently internalized into cells and is visible even after multiple divisions. We demonstrate that the internalized QD is nontoxic to cells and provides a sensitive tool for long-term molecular imaging.
Expression and bioactivity of recombinant segments of human perforinThis paper is one of a selection of papers in this Special Issue, entitled International Symposium on Recent Advances in Molecular, Clinical, and Social Medicine, and has undergone the Journal's usual peer-review process.
Authors: Dong Hongmei , Xu Xiaohu , Deng Mohong , Yu Xiaojun , Zhao Hu , Song Hui , Geng Yiqun
Published: May 2007

The aim of the study was to prepare an active recombinant human perforin by comparing 5 candidate segments of human perforin. Full-length perforin, MAC1 (28–349 aa), MAC2 (166–369 aa), C-100, and N-60 of human perforin were selected as candidate active segments and designated, respectively, HP1, HP2, HP3, HP4, and HP5. The target genes were amplified by PCR and the products were individually subcloned into pGEM-T. The genes for HP1, HP2, HP3, and HP5 were subcloned into pET-DsbA, whereas pET-41a (+) was used as the expression vector of HP4. The fusion proteins were expressed in Escherichia coli BL21pLysS(DE3) and purified using nickel nitrilotriacetic acid (NTA) agarose affinity chromatography. The hemolysis microassay was used as an activity assay of fusion protein. From this study, we obtained the recombinant plasmids pGEM-T-HP1, -HP2, -HP3, -HP4 and -HP5, consisting of 1600, 960, 600, 300bp, and 180, respectively. From these recombinant plasmids, expression plasmids were successfully constructed and expressed in E. coli BL21pLysS(DE3). The resultant fusion proteins, affinity purified using Ni–NTA, were ~80, 58, 45, 44, and 30 kDa, respectively. The recombinant proteins were assayed for activity on hemolysis. HP2 and HP5 were the only recombinant proteins that were active in hemolysis, and the hemolytic function was concentration dependent. These results demonstrate that active recombinant forms of perforin can be synthesized in a prokaryote model. The recombinant N-60 and MAC1 (28–349 aa) of human perforin have the function of forming pores. Our study provides the experimental basis for further investigation on the application of perforin.
Laboratory study on potential mechanisms for treatment of woodwaste leachate in surface flow constructed wetlands
Authors: Tao Wendong , Hall Ken , Hall Eric
Published: May 2007

Laboratory tests were conducted on volatilization, adsorption on silt loam, and on the biological treatability of woodwaste leachate. No significant volatilization was found despite the high concentration of volatile fatty acids. Tannin and lignin adsorption reached equilibration within 78 h. Adsorption at a silt loam:leachate volume ratio of 2:25, equivalent to a 2 cm effective soil layer underlying a 25 cm water column in a surface flow constructed wetland, removed 22–42% tannin and lignin and 6–27% chemical oxygen demand at 23 ºC. Tannin and lignin accounted for the majority of the adsorbed chemical oxygen demand. Adsorption followed the Langmuir isotherm, with an adsorption capacity of 36 mg tannin and lignin/g silt loam. Adsorption efficiency for tannin and lignin at 15 °C was 0.44 times that at 23 °C. An aerated reactor removed 62–67% chemical oxygen demand, 65–67% tannin and lignin, and ≥98% volatile fatty acids. A similar anaerobic reactor removed 28–29% chemical oxygen demand, 13–19% tannin and lignin, and 80–94% volatile fatty acids. In long-term operation, biological degradation would be the major mechanism for organic carbon removal from woodwaste leachate in surface flow constructed wetlands. Sediment adsorption would affect treatment performance mainly during initial and transitional operating periods.Key words: adsorption, biological treatability, volatilization, woodwaste leachate, surface flow constructed wetland.
On the qualities of the air as affected by radiant energies (photocatalytic ionization processes for remediation of indoor environments)
Authors: Daniels Stacy L
Published: May 2007

Airborne contaminants in indoor environments can affect the health of building occupants. Increased costs have prompted energy conservation and more stringent operational standards for buildings. Tight building envelopes reduce energy losses, but force reassessment of building designs and retrofits. The paradigm has shifted from "ventilation" to "comfort" to "indoor environmental quality" (IEQ). Various air treatment technologies can be used for control of trace contaminants. Conventional processes, such as sorption, filtration, and disinfection, are combined with advanced treatment processes (ATP), such as photocatalytic oxidation to oxidize volatile organic compounds (VOCs), bipolar air ionization to agglomerate particulate matter (PMx), and ultraviolet disinfection to inactivate bioaerosols. Modular hybrid devices are integrated into engineered systems to control low levels of these contaminants. The extensive early development of these technologies is supplemented with improved diagnostic techniques applied to domestic, institutional, and commercial installations. Key words: indoor environmental quality, IEQ, advanced treatment, ultraviolet, UV, titanium dioxide, TiO2, photocatalytic oxidation, PCO, volatile organic compounds, particulate matter, bioaerosols.
DETERMINATION OF TRACE ELEMENTS IN RIVER WATER SAMPLE BY PIXE
Authors: Tsuji M. , Kawasaki K. , Niizeki T. , Saitou M. , Hattori T.
Published: May 2007

Transition metal elements and others in river water sample supplied by The Japan Society for Analytical Chemistry (JSAC) were determined by PIXE using 2.5 MeV proton beam at the Tokyo Institute of Technology Van de Graaff Laboratory. The relative detection efficiency for each X-ray of element and its recovery were studied in two methods: "Drop and Dry" and "Concentration" methods. Ten elements ( V , Cr , Mn , Fe , Ni , Cu , Zn , As , Se and Pb ) were determined, being in good agreement with the values certified by JSAC except for Mn . Intensity of K α X-ray of As and L α, X-ray of Pb nearly close to each other could be successfully evaluated using isolated L β X-ray of Pb . Mn was insufficiently recovered to give lower content than the certified value in the present conditions. V that is not listed in the certificate was found to show good agreement with that reported previously. Concentration method using sodium dibenzyldithiocarbamate only could be successfully employed for collecting these elements.
Orexin-induced feeding requires NMDA receptor activation in the perifornical region of the lateral hypothalamus
Authors: Doane Dolores F. , Lawson Marcus A. , Meade Jonathan R. , Kotz Catherine M. , Beverly J. Lee
Published: May 2007

Food intake is stimulated following administration of orexin-A into the perifornical region of the lateral hypothalamus (LH/PFA). Orexin neurons originating in the LH/PFA interact with a number of hypothalamic systems known to influence food intake, including glutamatergic neurons. Glutamatergic systems in the LH/PFA were demonstrated to initiate feeding through N-methyl-d-aspartic acid (NMDA) receptors. Male Sprague-Dawley rats fitted with brain guide cannulas to the LH/PFA were used in two experiments. In the first experiment, a combination microdialysis/microinjection probe was used to deliver artificial cerebrospinal fluid (aCSF) or 500 pmol of orexin-A into the LH/PFA. Orexin-A increased interstitial glutamate to 143 ± 12% of baseline ( P < 0.05), which remained elevated over the 120-min collection period. In the second experiment, the NMDA receptor antagonist d-2-amino-5-phosphonopentanoic acid (d-AP5; 10 nmol) was administered before orexin-A. The orexin-induced increase in food intake (from 1.1 ± 0.4 to 3.2 ± 0.5 g, P < 0.05) during the first hour was absent in rats receiving d-AP5 + orexin-A (1.2 ± 0.5 g). There was no effect of d-AP5 alone on food intake. These data support glutamatergic systems in the LH/PFA mediating the feeding response to orexin-A through NMDA receptors.
Anthropic acceleration of a natural clay mineral reaction in marshland soils (Atlantic Coast, France)
Authors: Mathé V. , Meunier A. , Lévêque F.
Published: May 2007

Soil clay minerals in recent natural polders react on a human timescale in response to local environmental conditions. With increasing age, the mineral reaction leads to the dissolution of the chlorite component and a composition change of the different illite-smectite mixed-layer minerals (I-S MLMs): i.e. smectite layer content decreases and illite content increases. The process of oxidation, which is proven by magnetic susceptibility to trigger clay mineral reaction, changes the mineralogical composition of the sediment above the redox front. The mineral changes appear to be a non-linear function of time. In natural conditions the process lasts >1000 y. However, anthropoic forcing such as artificial drainage accelerates the oxidation reaction to complete the whole process in a few tens of years.
Transformation of magnetite to goethite under alkaline pH conditions
Authors: He Y. T. , Traina S. J.
Published: May 2007

Magnetite is a redox active Fe oxide common in most soil and sedimentary environments. In this study, we investigated magnetite transformations under extreme alkaline conditions (0.1–2 mol l–1 NaOH) similar to those found under high-level radioactive waste storage tanks at the Hanford site in the State of Washington, USA. X-ray diffraction (XRD) and transmission electron microscopy (TEM) results showed that magnetite was transformed into maghemite and goethite, and the XRD peak intensities for goethite increased with NaOH concentration. Goethite was presumably formed through reconstructive dissolution/crystallization reactions that can be written as: Fe3O4 + OH– + H2O = γ-Fe2O3 + Fe(OH)3–; 2Fe(OH)3– + 1/2O2 + 2H+ = 2α-FeOOH + 3H2O. Some of the newly formed maghemite may also redissolve to form goethite at greater NaOH concentrations.
Newly formed phyllosilicates in rock matrices and fractures from CRP-3 core (Antarctica): an electron microscopy study
Authors: Giorgetti G. , Aghib F. S. , Livi K. J. T. , Gaillot A.-C. , Wilson T. J.
Published: May 2007

A scanning and transmission electron microscopy study has been performed on Oligocene glacio-marine sediments, Devonian sandstones, and Jurassic dolerites recovered during CRP-3 drilling in the Ross Sea (Antarctica). Newly formed clay minerals occur in the rock matrices and as fillings in veins and faults which crosscut the whole sequence. Authigenic clays in sediments consist of beidellite-montmorillonite, berthierine/chlorite intergrowths and illite. Al,K-rich smectites and kaolinite occur in the Devonian sandstones. Saponite, berthierine/chlorite intergrowths, and Fe-hydroxides develop in the altered dolerites. Hence, the composition of the secondary phases depends also on the geochemistry of the rock they grow in. Within each sample, the same authigenic minerals form in the matrix and in the vein/fault. Clays precipitated from fluids, with variable fO2 values, which circulated in the system during the contemporaneous diagenetic and faulting events.
Authigenic palygorskite in Miocene sediments in Linxia basin, Gansu, northwestern China
Authors: Hong H. L. , Yu N. , Xiao P. , Zhu Y. H. , Zhang K. X. , Xiang S. Y.
Published: May 2007

The mineralogical characteristics of authigenic palygorskite occurring with chlorite and illite in Miocene sediments in Linxia basin were investigated using X-ray diffraction (XRD), scanning electron microscopy (SEM), and high-resolution transmission electron microscopy (HRTEM). The XRD results suggest that the mineral composition of the sediments includes mainly quartz, chlorite, illite, calcite, plagioclase, orthoclase, minor palygorskite and small amounts of gypsum and ankerite. Two kinds of palygorskite were observed in the sediments, relatively straight fibrous particles found in matted, felted masses associated with platy chlorite and silky aggregates found in the void spaces. The former probably replaces chlorite grains, growing from the edges or the fissures of chlorite particles. Chlorite grains exhibit bay-shaped or rounded edges, with ambiguous felted boundaries between chlorite particles, indicative of an intensive dissolution process and the growth of palygorskite at the expense of chlorite. Palygorskite is also observed as inclusions within calcite, sprouting from or coating calcite surfaces, suggesting that palygorskite crystallized from solution. The textural relations of palygorskite and the occurrence of ankerite and the characteristically Fe-bearing palygorskite in the sediments suggest the destruction and hydrolysis of chlorite. The ankerite seems to be preferentially present in the void spaces, closely associated with chlorite and illite; fibrous palygorskite crystallizes at the edges of these clay mineral particles and the platy clay mineral particles are gradually replaced by fibrous palygorskite crystals, suggesting that alteration of chlorite to palygorskite involves an interaction with water during the diagenetic process.
Preparation and characterization of organic rectorite composite gel polymer electrolyte
Authors: Huang Y. , Ma X. Y. , Liang G. Z. , Yan H. X. , Qu X. , Chen F.
Published: May 2007

In liquid-filled batteries, the liquid electrolytes may escape or present a fire hazard and an inert spacer is needed to separate the electrodes. Alternative polymer-based electrolytes are of current technological interest. Solid polymer electrolytes are non-volatile, non-corrosive materials, which can readily be processed into any shape or size. However, despite possessing the required mechanical properties, they have inherently lower conductivity. Gel-based systems are an attempt to strike a balance between the high conductivity of organic liquid electrolytes and the dimensional stability of a solid polymer.

Rectorite was modified with dodecyl benzyl dimethyl ammonium chloride to form organic-modified rectorite (OREC). OREC was used as a filler additive to modify gel polymer electrolytes (GPEs) and prepare composite gel polymer electrolytes (CPEs) which consisted of polymethyl methacrylate (PMMA) used as a polymer matrix, propylene carbonate (PC), used as a plasticizer, and LiClO4, used as a lithium ion producer. A variety of physical and chemical techniques was used to characterize the CPEs. The interlayer d spacing of OREC was much larger than that of the initial rectorite (2.22 nm). OREC also possesses a fine microscopic structure, and has a hydrophobic surface. Molau and XRD analysis of CPEs indicate that OREC has good compatibility with the components of CPEs and can be dispersed well. The effects of temperature and OREC dose on properties were studied. The temperature dependence of ionic conductivity of CPEs is well fitted by the VTF (Vogel-Tamman-Fulcher) relation. OREC doses of 5 phr gave the greatest ionic conductivity. This amount also greatly increased the plasticizer maintenance levels. Due to the occupancy of free volume space in the polymer matrix of CPEs by OREC, the bulk resistance of the CPEs was lowered and the glass transition temperature (Tg) increased. The sheet structure of OREC is thought to improve the decomposition temperature of CPEs.
Mineralogical, micromorphological and geochemical evolution of the kaolin facies deposit from the Capim region (northern Brazil)
Authors: Sousa D. J. L. , Varajão A. F. D. C. , Yvon J. , Da Costa G. M.
Published: May 2007

The Capim Kaolin District (eastern Brazilian Amazon), is one of the largest kaolin deposits in the world; with the kaolin used mainly for paper coating. The kaolin developed at the expense of Cretaceous sandy-clayey sediments of the Ipixuna formation, through intense lateritization from the Mesozoic to Cenozoic times.

This work describes the morphological, mineralogical, crystallochemical and geochemical evolution of the Capim kaolin facies. Based on the profile analysis in the open pit fronts, it encompasses X-ray diffraction, thin-section optical analysis, EDS-assisted scanning electron microscopy and transmission electron microscopy, chemical analysis, infrared and Mössbauer spectroscopies.

Six facies were defined as different stages of the supergene process. Ferruginization led to a thick duricrust on the soft kaolin, which in turn evolved from sandy-clayey sediments of the Ipixuna Formation. A subsequent deferruginization event degraded the duricrust, resulting in the flint kaolin facies.
Natural acid sulphate alteration in bentonites (Cabo de Gata, Almeria, SE Spain)
Authors: Martínez J. A. , Jiménez de Cisneros C. , Caballero E.
Published: May 2007

This study is located on the bentonite outcrop of ‘El Toril’ (Cabo de Gata, Almeria, Spain), where acid solutions have resulted in an alteration front that has caused physical, chemical and mineralogical changes in the bentonite material. The main objective is to assess these changes and to draw a model of evolution of this front in the outcrop. The most important chemical and mineralogical changes observed consist mainly of the loss of mass in the bentonite, the formation of kaolinite, the precipitation of jarosite and alunite from leachates and important changes in the rare earth element distribution pattern. No sulphurs have been identified in the pyroclastic rock at the top of the outcrop, but the presence of dissolution marks and the high concentrations of As, Pb and V in the rock overlying the bentonite point towards the existence, in principle, of these sulphurs and suggest that meteoric alteration is mainly responsible for the genesis of the outcrop.
Prograde epizonal clay mineral assemblages and retrograde alteration in tectonic basins controlled by major strike-slip zones (W Iberian Variscan chain)
Authors: Vázquez M. , Abad I. , Jiménez-Millán J. , Rocha F. T. , Fonseca P. E. , Chaminé H. I.
Published: May 2007

We have carried out optical microscopy, X-ray diffraction (XRD) and scanning and transmission electron microscopy (SEM and TEM) studies of phyllosilicates from black slates of very low to low-grade metamorphism. Such slates belong to a Middle/Late Devonian basin and an Early Carboniferous basin associated with the Porto–Tomar–Ferreira do Alentejo strike-slip shear zone (Ossa-Morena Zone, Portuguese Iberian Variscan Massif). These black slates are imbricated in an Upper Proterozoic substratum of higher metamorphic grade. Kübler Index values of white micas and mineral assemblages deduced from the XRD, SEM and TEM data (muscovite, chlorite and pyrophyllite) indicate high anchizonal and epizonal metamorphic conditions for slates from these basins. The b parameter and the phengitic contents of mica suggest the occurrence of low pressures (1–2 kbar) related to an extensional geotectonic setting. The dense fracture network shown by SEM images and the high density of crystal defects revealed by the TEM study in the eastern basin, adjacent to faults produced by shearing, suggest that their epizonal phyllosilicates were more affected during deformation than those belonging to the western basin, favouring the development of a retrograde association (siderite, kaolin group minerals and Al-smectite) on the epizonal paragenesis. Microcavities formed along phyllosilicate cleavage acted as channels for fluid transport favouring alteration under low-temperature conditions.
Integration of Fe in natural and synthetic Al-pyrophyllites: an infrared spectroscopic study
Authors: Lantenois S. , Beny J.-M. , Muller F. , Champallier R.
Published: May 2007

Numerous studies focus on the relationships between chemical composition and OH-band positions in the infrared (IR) spectra of micaceous minerals. These studies are based on the coexistence, in dioctahedral micas or smectites, of several cationic pairs around the hydroxyl group which each produce a characteristic band in the IR spectrum. The aim of this work is to obtain the wavenumber values of the IR OH vibration bands of the (Al-Fe3+)-OH and (Fe3+-Fe3+)-OH local cationic environments of ‘pyrophyllite type’ in order to prove, disprove or modify a model of dioctahedral phyllosilicate OH-stretching band decomposition. Natural samples are characterized by powder X-ray diffraction (XRD), Fourier transform infrared (FTIR) and Raman spectroscopies and electron microprobe; the hydrothermal synthesis products are also analysed by powder XRD and FTIR after inductively coupled plasma measurements to obtain the chemical compositions of starting gel phases. Natural samples contain some impurities which were eliminated after acid treatment; nevertheless, a small Fe content is found in the pyrophyllite structure. The amount of Fe which is incorporated within the pyrophyllite structure is much more important for the synthetic samples than for the natural ones. The IR OH bands were clearly observed in both natural and synthetic pyrophyllites and assigned to hydroxides bonded to (Al-Al), (Al-Fe) and (Fe-Fe) cationic pairs. During this study, three samples were analysed by DTG to check the cis- or trans-vacant character of the layers and to determine the influence of this structural character on the OH-stretching band position in IR spectroscopy.
NPM/ALK binds and phosphorylates the RNA/DNA-binding protein PSF in anaplastic large-cell lymphoma
Authors: Galietta Annamaria , Gunby Rosalind H. , Redaelli Sara , Stano Paola , Carniti Cristiana , Bachi Angela , Tucker Philip W. , Tartari Carmen J. , Huang Ching-Jung , Colombo Emanuela , Pulford Karen , Puttini Miriam , Piazza Rocco G. , Ruchatz Holger , Villa Antonello , Donella-Deana Arianna , Marin Oriano , Perrotti Danilo , Gambacorti-Passerini Carlo
Published: May 2007

The oncogenic fusion tyrosine kinase nucleophosmin/anaplastic lymphoma kinase (NPM/ALK) induces cellular transformation in anaplastic large-cell lymphomas (ALCLs) carrying the t(2;5) chromosomal translocation. Protein-protein interactions involving NPM/ALK are important for the activation of downstream signaling pathways. This study was aimed at identifying novel NPM/ALK-binding proteins that might contribute to its oncogenic transformation. Using a proteomic approach, several RNA/DNA-binding proteins were found to coimmunoprecipitate with NPM/ALK, including the multifunctional polypyrimidine tract binding proteinassociated splicing factor (PSF). The interaction between NPM/ALK and PSF was dependent on an active ALK kinase domain and PSF was found to be tyrosine-phosphorylated in NPM/ALK-expressing cell lines and in primary ALK+ ALCL samples. Furthermore, PSF was shown to be a direct substrate of purified ALK kinase domain in vitro, and PSF Tyr293 was identified as the site of phosphorylation. Y293F PSF was not phosphorylated by NPM/ALK and was not delocalized in NPM/ALK+ cells. The expression of ALK fusion proteins induced delocalization of PSF from the nucleus to the cytoplasm and forced overexpression of PSF-inhibited proliferation and induced apoptosis in cells expressing NPM/ALK. PSF phosphorylation also increased its binding to RNA and decreased the PSF-mediated suppression of GAGE6 expression. These results identify PSF as a novel NPM/ALK-binding protein and substrate, and suggest that PSF function may be perturbed in NPM/ALK-transformed cells.
Central administration of dipeptides, beta-alanyl-BCAAs, induces hyperactivity in chicks
Authors: Tsuneyoshi Yousuke , Tomonaga Shozo , Asechi Mari , Morishita Koji , Denbow D Michael , Furuse Mitsuhiro
Published: May 2007

Background

Carnosine (β-alanyl-L-histidine) is a putative neurotransmitter and has a possible role in neuron-glia cell interactions. Previously, we reported that carnosine induced hyperactivity in chicks when intracerebroventricularly (i.c.v.) administered. In the present study, we focused on other β-alanyl dipeptides to determine if they have novel functions.

Results

In Experiment 1, i.c.v. injection of β-alanyl-L-leucine, but not β-alanyl-glycine, induced hyperactivity behavior as observed with carnosine. Both carnosine and β-alanyl-L-leucine stimulated corticosterone release. Thus, dipeptides of β-alanyl-branched chain amino acids were compared in Experiment 2. The i.c.v. injection of β-alanyl-L-isoleucine caused a similar response as β-alanyl-L-leucine, but β-alanyl-L-valine was somewhat less effective than the other two dipeptides. β-Alanyl-L-leucine strongly stimulated, and the other two dipeptides tended to stimulate, corticosterone release.

Conclusion

These results suggest that central β-alanyl-branched chain amino acid stimulates activity in chicks through the hypothalamus-pituitary-adrenal axis. We named β-alanyl-L-leucine, β-alanyl-L-isoleucine and β-alanyl-L-valine as Excitin-1, Excitin-2 and Excitin-3, respectively.
Sensitivity and specificity of monoclonal and polyclonal immunohistochemical staining for West Nile virus in various organs from American crows (Corvus brachyrhynchos)
Authors: Smedley Rebecca C , Patterson Jon S , Miller RoseAnn , Massey Jeffrey P , Wise Annabel G , Maes Roger K , Wu Ping , Kaneene John B , Kiupel Matti
Published: May 2007

Background

Based on results of earlier studies, brain, heart and kidney are most commonly used for West Nile virus (WNV) detection in avian species. Both monoclonal and polyclonal antibodies have been used for the immunohistochemical diagnosis of WNV in these species. Thus far, no studies have been performed to compare the sensitivity and specificity of monoclonal and polyclonal antibodies in detecting WNV in American crows (Corvus brachyrhynchos). Our objectives were to determine 1) the comparative sensitivities of monoclonal and polyclonal antibodies for immunohistochemical (IHC) diagnosis of WNV infection in free-ranging American crows, 2) which organ(s) is/are most suitable for IHC-based diagnosis of WNV, and 3) how real-time RT-PCR on RNA extracted from formalin-fixed paraffin-embedded tissues compared to IHC for the diagnosis of WNV infection.

Methods

Various combinations, depending on tissue availability, of sections of heart, kidney, brain, liver, lung, spleen, and small intestine from 85 free-ranging American crows were stained using a rabbit-polyclonal anti-WNV antibody as well as a monoclonal antibody directed against an epitope on Domain III of the E protein of WNV. The staining intensity and the extent of staining were determined for each organ using both antibodies. Real-time RT-PCR on formalin-fixed paraffin-embedded tissues from all 85 crows was performed.

Results

Forty-three crows were IHC-positive in at least one of the examined organs with the polyclonal antibody, and of these, only 31 were positive when IHC was performed with the monoclonal antibody. Real-time RT-PCR amplified WNV-specific sequences from tissue extracts of the same 43 crows that were IHC-positive using the polyclonal antibody. All other 42 crows tested negative for WNV with real-time PCR and IHC staining. Both antibodies had a test specificity of 100% when compared to PCR results. The test sensitivity of monoclonal antibody-based IHC staining was only 72%, compared to 100% when using the polyclonal antibody.

Conclusion

The most sensitive, readily identified, positively staining organs for IHC are the kidney, liver, lung, spleen, and small intestine. Real-time RT-PCR and IHC staining using a polyclonal antibody on sections of these tissues are highly sensitive diagnostic tests for the detection of WNV in formalin-fixed tissues of American crows.
Length of gestation and depressive symptoms at age 60 years
Authors: Räikkönen Katri , Pesonen Anu-Katriina , Kajantie Eero , Heinonen Kati , Forsén Tom , Phillips David I. W. , Osmond Clive , Barker David J. P. , Eriksson Johan G.
Published: May 2007

Background

A non-optimal foetal environment, reflected in smaller birth size and shorter duration of gestation, is a risk factor for compromised health later in life.

Aims

To examine whether smaller birth size and shorter gestation predict depressive symptoms.

Method

A total of 1371 members of a cohort born between 1934 and 1944 at term (259–294 days' gestation) in Helsinki, Finland, completed the Beck Depression Inventory (BDI) and the Center for Epidemiological Studies Depression scale (CES–D) at an average age of 61.5 years (BDI) and 63.4 years (BDI and CES–D).

Results

Gestational length predicted depressive symptoms linearly and independently of gender and birth weight: per day decrease in gestational length, depressive symptoms scores increased by 0.8-0.9% (95% CI 0.2-1.4, P<0.009). Weight, length and head circumference at birth showed no linear association with depression, adjusted for gender and gestational length. The results did not change when further controlled for socioeconomic characteristics at birth and in adulthood, age and body mass index in adulthood.

Conclusions

Susceptibility to depressive symptoms may relate to shorter length of gestation.
Substance use in a population-based clinic sample of people with first-episode psychosis
Authors: Barnett Jennifer H. , Werners Ursula , Secher Sandra M. , Hill Katherine E. , Brazil Rossa , Masson Kim , Pernet David E. , Kirkbride James B. , Murray Graham K. , Bullmore Ed. T. , Jones Peter B.
Published: May 2007

Background

Substance use is implicated in the cause and course of psychosis.

Aims

To characterise substance and alcohol use in an epidemiologically representative treatment sample of people experiencing a first psychotic episode in south Cambridgeshire.

Method

Current and lifetime substance use was recorded for 123 consecutive referrals to a specialist early intervention service. Substance use was compared with general population prevalence estimates from the British Crime Survey.

Results

Substance use among people with first-episode psychosis was twice that of the general population and was more common in men than women. Cannabis abuse was reported in 51% of patients (n=62) and alcohol abuse in 43% (n=53). More than half (n=68, 55%) had used Class A drugs, and 38% (n=43) reported polysubstance abuse. Age at first use of cannabis, cocaine, ecstasy and amphetamine was significantly associated with age at first psychotic symptom.

Conclusions

Substance misuse is present in the majority of people with first-episode psychosis and has major implications for management. The association between age at first substance use and first psychotic symptoms has public health implications.
Salmonella ochiogu: experimental infection of laboratory mice and oxytetracycline therapy
Authors: Onyekaba C. O. , Oranusi N. A.
Published: May 2007

The oral infection of laboratory mice with 108 colony-forming units of viable Salmonella ochiogu bacteria resulted in clinical salmonellosis and death in 10 out of 45 of the mice (22%). None of the mice treated with oxytetracycline died. Infection in susceptible mice was characterized by septicaemia, respiratory involvement and mild enteritis. The organism was shed in the faeces from the first day after infection until day 30, and cultures from viscera showed systemic dissemination. S. ochiogu was recovered from the faeces of mice treated with oxytetracycline between days 1 and 9 post infection.
The congenic normal R/APfd and jaundiced R/APfd-j/j rat strains: a new animal model of hereditary non-haemolytic unconjugated hyperbilirubinaemia due to defective bilirubin conjugation
Authors: Leyten R. , Vroemen J. P. A. M. , Blanckaert N. , Heirwegh K. P. M.
Published: May 2007

In this paper the production of the R/APfd-j/j strain which is congenic with the R/APfd strain is reported. The R/APfd-j/j completely lacks hepatic bilirubin UDP-glucuronyltransferase activity, as do our GUNNXR/Pfd-j/j rat strain and various other stocks of GUNN rats (j/j) described in the literature. Our recombinant inbred strain GUNNXR/Pfd-j/j was produced from non-inbred GUNN (j/j) rats. This GUNNXR/Pfd-j/j rat was used as a donor of the jaundice gene j, the R/APfd rat serving as the recipient. After eight backcross-intercross cycles (16 generations) the R/APfd-j/j strain was obtained which is congenic with the R/APfd strain. Congenicity was demonstrated by various techniques including transplantation of skin tissue, strain-specific tumour cells and hepatocytes, the mixed lymphocyte reaction, and comparison of biochemical markers. The potential of the novel inbred strain of jaundiced rat, R/APfd-j/j, and the corresponding control strain R/APfd for biochemical and clinical studies of bilirubin metabolism are briefly discussed.
Aqueous lithium heparin is a superior anticoagulant to solid heparin for blood collection from the retro-orbital sinus of rats
Authors: Slaughter M. R. , Moen J. S.
Published: May 2007

Blood specimens from the retro-orbital sinus of 80 Sprague Dawley rats were collected into tubes containing lithium heparin either as a solid or an aqueous solution. Plasma was separated for blood chemistry analysis. Twenty-eight blood specimens collected into tubes containing solid heparin were clotted and eight specimens were partially clotted making these samples unsuitable for some analyses. None of the specimens collected into heparin solution showed any evidence of clotting. The variances of lactate dehydrogenase and α-hydroxybutyrate dehydrogenase activities in plasma prepared with solid heparin were significantly greater than those prepared with heparin solution. Lithium heparin solution is now used routinely in our laboratory.
Evaluation of isolator caging systems for protection of mice against challenge with mouse hepatitis virus
Authors: Lipman N. S. , Corning B. F. , Saifuddin MD
Published: May 2007

Two isolator caging systems were evaluated against challenge with MHV-Y, an enterotropic strain of mouse hepatitis virus. The systems were similar in that they both used an identical shoebox cage equipped with a polycarbonate filter top incorporating a Reemay® filter. They differed in that one system supplied HEPA-filtered air through a grommet in the filter lid so that the cage was pressurized slightly. A rack holding 60 cages (30 front and back) was utilized. Thirty cages without filter tops housed one mouse each that had been infected orally with 19000 ID50 of MHV-Y and an uninfected cagemate. The remaining 30 cages, each housing 2 uninfected mice were divided into 3 groups of 10 cages. Group I cages (controls) had no filter top; Group II cages were equipped with filter tops; and Group III were equipped with filter tops and intracage HEPA-filtered air. The cages housing uninfected mice were interspersed between, above, below and behind cages housing infected mice. The uninfected mice were maintained in contact with the MHV-Y infected mice for 8 weeks. Transmission of MHV-Y was determined serologically by indirect ELISA. All mice housed within the Group I cages (control) seroconverted to MHV, while only 4 mice (2 cages) seroconverted in Group II, and no mice seroconverted in Group III
Skin ageing
Authors: Calleja-Agius J , Muscat-Baron Y , Brincat M P
Published: May 2007

Cutaneous ageing manifests itself as a progressive reduction in maximum function and reserve capacity of skin tissue. It is not a unique and uniform biological event. Skin comprises three layers: epidermis, dermis and subcutaneous tissue. Collagen atrophy is a major factor in skin ageing. There is a strong correlation between skin collagen loss and estrogen deficiency due to the menopause. Skin ageing, especially in the face, is associated with a progressive increase in extensibility and a reduction in elasticity. With increasing age, the skin also becomes more fragile and susceptible to trauma, leading to more lacerations and bruising. Furthermore, wound healing is impaired in older women. Estrogen use after the menopause increases collagen content, dermal thickness and elasticity, and it decreases the likelihood of senile dry skin. Large-scale clinical trials are necessary to help make informed recommendations regarding postmenopausal estrogen use and its role in the prevention of skin ageing.
Exercise-related menstrual dysfunction: implications for menopausal health
Authors: Speed Cathy
Published: May 2007

Physical activity is a well recognized approach to the enhancement of general health and wellbeing in both healthy and diseased populations. However, excessive activity can result in exercise-related menstrual dysfunction (ERMD), which can adversely affect bone health and increase the risk of infertility and cardiovascular events in later life. Physicians should maintain a high awareness of EMRD in assessing health risks in the menopausal woman.
Bisphosphonate-associated osteonecrosis of the jaw
Authors: Basu Neil , Reid David M
Published: May 2007

The term 'osteonecrosis of the jaw' is typically used to describe the exposure of bone within the oral cavity. These lesions are often painful and can result in pathological fracture and disfigurement in severe cases. Infection and dental extractions commonly precede presentation, although lesions can occur spontaneously. There are no clear effective management strategies; surgery often makes the situation worse. The aetiology is far from clear. Historically, it was mainly associated with exposure to 'white phosphorus' contained in safety matches. More recently, it has presented as a complication of head and neck radio-therapy. The vast majority of contemporary reports refer to high-dose intravenous bisphosphonates used in the oncological setting. However, oral bisphosphonates, for the common indication of postmenopausal osteoporosis, have also been implicated, although the number of cases is minuscule considering the number of worldwide prescriptions. Osteonecrosis of the jaw poses a significant problem in oncology, but whether this worry is transferable to metabolic bone disease only time will tell. Certainly, further research into its pathophysiology and management is merited. With regard to management of postmenopausal osteoporosis, practice should not necessarily be altered at this early stage; however, pre-treatment worries regarding dental health should perhaps instigate a precautionary dental review.
Dehydroepiandrosterone (DHEA) and the menopause: an update
Authors: Raven P W , Hinson J P
Published: May 2007

Since we last reviewed this topic in 2001, considerably more information about dehydroepiandrosterone (DHEA) has accrued, but this has not necessarily left us any wiser about the use of this steroid in postmenopausal women. There is no further evidence that DHEA supplementation is likely to be useful in the prevention of cardiovascular disease or cognitive impairment, or in the promotion of wellbeing. Evidence has, however, accumulated for beneficial effects of DHEA on osteoporosis, both in postmenopausal women and in patients receiving long-term glucocorticoid therapy. What is also emerging is a link between low DHEA levels and cardiovascular risk, and between high DHEA levels and breast cancer risk. In fact, the benefits and adverse effects of DHEA administration in postmenopausal women increasingly resemble those of conventional hormone replacement therapy. Overall, we conclude that DHEA is not currently to be recommended for therapeutic use in the majority of postmenopausal women. However, DHEA supplementation may be of benefit in two specific groups of women: those with the lowest circulating levels of DHEA; and those for whom osteoporosis is a particular problem.
Improving quality of life after breast cancer: prevention of other diseases
Authors: Rozenberg Serge , Antoine Caroline , Carly Birgit , Pastijn Ann , Liebens Fabienne
Published: May 2007

Many women with breast cancer will be diagnosed at an early stage through screening programmes. Furthermore, most women affected by breast cancer will not die from it but from other diseases, owing to recent improvements in treatment. This article assesses whether breast cancer survivors suffer more frequently from other diseases. Specifically, it examines whether they have a higher incidence of other cancers, cardiovascular events and osteoporotic fractures. Women with breast cancer and three or more co-morbid conditions have a 20-fold higher rate of mortality from causes other than breast cancer and a 4-fold higher rate of all-cause mortality when compared with patients who have none. Breast cancer survivors are at increased risk of other cancers, such as stomach, colorectal and lung. Radiotherapy, trastuzumab and aromatase inhibitors increase the risk of cardiovascular disease. However, tamoxifen does not, although it is associated with an increased risk of venous thromboembolism. Aromatase inhibitors, but not tamoxifen, increase the risk of osteoporotoic fracture and bisphosphonate therapy should be considered.
Home care needs of extremely obese elderly European women
Authors: Sørbye L W , Schroll M , Finne-Soveri H , Jónnson P V , Ljunggren G , Topinkova E , Bernabei R ,   The AdHOC Project Research Group
Published: May 2007

Objective. To examine the health and needs of extremely obese women aged over 65 years receiving home care in Europe.

Study design. A cross-sectional assessment study based on the Aged in Home Care (AdHOC) project recruited 2974 women aged 65 or over who were receiving home care at 11 sites in European countries. Extreme obesity was defined as 'Obesity of such a degree as to interfere with normal activities, including respiration'.

Main outcome measures: Resident Assessment Instrument for Home Care (RAI-HC version 2.0); Activity of Daily Living Scale; Instrumental Activity of Daily Living Scale; the Minimum Data Set Cognitive Performance Scale; and a health profile.

Results: One hundred and twenty women (4.0%) were extremely obese. They were younger than their thinner counterparts, with a median age of 78.3 versus 83.3 years, and they more often had multiple health complaints and needed more help with mobility outside the home. The extremely obese had received home care longer than the non-extremely obese (median 28.7 versus 36.6 months). Extremely obese women also needed more help with personal care than the other group and, due to lower age, they were less cognitively impaired.

Conclusions: Extreme obesity is a problem that increasingly affects home care of elderly women.
Women's attitudes to hormone replacement therapy, alternative therapy and sexual health: a web-based survey
Authors: Cumming Grant P , Herald Julie , Moncur Rik , Currie Heather , Lee Amanda J
Published: May 2007

Objective. To survey women's views on hormone replacement therapy (HRT), alternative therapies and sexual health using the Internet.

Study design and main outcome measures. Three questionnaires were offered on a UK, patient-tailored, independent, clinician-led dedicated menopause website. They covered HRT, alternative therapies and sexual health. The anonymous responses of the users of the website were analysed.

Results. There were 1026, 1072 and 1002 responses for the HRT, alternative therapies and sexual health questionnaires, respectively. On the first, 75% of respondents were in favour of HRT; 36% felt media reports of the risks of HRT had been exaggerated and 73% of women did not know enough about HRT to make informed choices. In relation to alternative therapies, 85% of respondents felt they did not know enough to make informed choices, 71% received no advice before starting an alternative therapy and 69% were unaware of possible interactions. Ninety-five per cent would try alternative therapies before HRT in the belief that they were more natural and 68% were prepared to pay more than £10 a month for such therapies. On the questionnaire on sexual health, 88% of respondents indicated that they believed an active sex life was important. Fifty-three per cent recorded that they experienced dyspareunia, but 51% of women hid their symptoms and 31% made excuses to avoid intercourse; 54% felt their confidence had been adversely affected. Only 20% had discussed their symptoms with health professionals and only 12% were using prescribed treatment.

Conclusions. Online questionnaires are a useful means to obtain data. Our surveys raised several issues, including the observations that the majority of women said they did not know enough about HRT and alternative therapies to make informed choices. There appeared to be many women with vaginal symptoms who had not spoken with a health professional and therefore were untreated.
Systemic lupus erythematosus and hormone replacement therapy
Authors: Gompel A , Piette J C
Published: May 2007

The indications for hormone replacement therapy (HRT) in postmenopausal women is the treatment of climacteric symptoms and the prevention of osteoporosis. Women with systemic lupus erythematosus (SLE) are more likely to have a premature menopause, osteoporosis and cardiovascular disease. HRT can induce SLE flares and cardiovascular or venous thromboembolic events. Therefore it should not be used in women with active disease or those with antiphospholipid (aPL) antibodies. In general, it should be used only for patients without active disease, a history of thrombosis or aPL antibodies. Non-oral administration of estrogen is recommended because of its lesser effect on coagulation. With regard to the progestogen, progesterone or pregnane derivatives are preferred. Otherwise, non-estrogen-based strategies should be used.