bluuebunny/crossref_metadata_2025 huggingface dataset:
The Influence of Fluoride Gels on the Physicochemical Properties of Tooth Tissues and Dental Materials—A Systematic Review
Authors: Piszko Paweł J. , Piszko Aleksandra , Kiryk Jan , Lubojański Adam , Dobrzyński Wojciech , Wiglusz Rafal J. , Matys Jacek , Dobrzyński Maciej
Published: January 2024

The aim of the presented systematic review is to update the state of knowledge and relate the properties and composition of fluoride gels to their potential application. This article aims to explore the effect of fluoride gel application on changes in the properties of dental biomaterials and tooth tissues. The review includes articles assessing studies on the effects of fluoride gel on dental tissues and materials. Employing the PRISMA protocol, a meticulous search was conducted across the PubMed, Scopus, and Web of Science databases, utilizing keywords such as fluoride, gel, and properties. The publications were selected without limitation by the year of publication, and then Cohen’s κ test was used to assess the agreement of the respondents. Exclusion criteria included non-English studies, opinion pieces, editorial papers, letters to the editor, review articles and meta-analyses, clinical reports, studies lacking full-text accessibility, and duplicates. The quality of the chosen papers was assessed by two independent reviewers. A total of 2385 were located in databases, of which only 17 met the inclusion criteria. All publications showed increased surface mineralization, and seven studies showed the effect of fluoride gel on the surface of dental tissues. Three articles stated a negative effect of fluoride gels on titanium and stainless steel alloys and glass ionomer fillings. The effects on shear bond strength and plaque deposition require further investigation because the study results are contradictory.
Identifying the Barriers to Acceptance of Blockchain-Based Patient-Centric Data Management Systems in Healthcare
Authors: Mutambik Ibrahim , Lee John , Almuqrin Abdullah , Alharbi Zahyah H.
Published: January 2024

A number of recent studies have shown that wastage and inefficiency are a significant problem in all global healthcare systems. One initiative that could radically improve the operational efficiency of health systems is to make a paradigm shift in data ownership—that is, to transition such systems to a patient-centric model of data management by deploying blockchain technology. Such a development would not only make an economic impact, by radically cutting wastage, but would deliver significant social benefits by improving patient outcomes and satisfaction. However, a blockchain-based solution presents considerable challenges. This research seeks to understand the principal factors, which act as barriers to the acceptance of a blockchain-based patient-centric data management infrastructure, in the healthcare systems of the GCC (Gulf Cooperation Council) countries. The study represents an addition to the current literature by examining the perspectives and views of healthcare professionals and users. This approach is rare within this subject area, and is identified in existing systematic reviews as a research gap: a qualitative investigation of motivations and attitudes among these groups is a critical need. The results of the study identified 12 key barriers to the acceptance of blockchain infrastructures, thereby adding to our understanding of the challenges that need to be overcome in order to benefit from this relatively recent technology. The research is expected to be of use to healthcare authorities in planning a way forward for system improvement, particularly in terms of successfully introducing patient-centric systems.
The Influence of Nurse-Led Interventions on Diseases Management in Patients with Diabetes Mellitus: A Narrative Review
Authors: Dailah Hamad Ghaleb
Published: January 2024

The global prevalence of people with diabetes mellitus (PWD) is rapidly increasing. Nurses can provide diabetes care for PWD in several areas. Interventions led by nurses can support PWD for effective management of diabetes, which can positively improve clinical outcomes. Nurse-led diabetes self-management education (DSME) is an effective strategy to manage diabetes mellitus (DM) since it improves self-care practice and knowledge regarding diabetes. PWD often need to stay in hospitals longer, which involves poorer patient satisfaction and clinical outcomes. Nurse-led clinics for DM management are a new strategy to possibly ameliorate the disease management. Diabetes specialist nurses can play an important role in improving diabetes care in inpatient settings. Various studies have revealed that nurses can independently provide care to PWD in collaboration with various other healthcare providers. Studies also demonstrated that the nurse-led education-receiving group showed a significantly reduced level of average glycosylated haemoglobin A1c level. Moreover, nurse-led interventions often result in significant improvements in diabetes knowledge, psychological outcomes, self-management behaviours, and physiological outcomes. The purpose of this literature review was to identify the impact of nurse-led interventions on diabetes management. Moreover, in this review, a number of nursing interventions and the nurses’ roles as educators, motivators as well as caregivers in DM management have been extensively discussed. This article also summarises the outcomes that are measured to evaluate the impact of nursing interventions and the strategies to overcome the existing and emerging challenges for nurses in diabetes care.
Can Virtual Reality Help Improve Motor and Cognitive Function in Active Aging in Older Adults? A Scoping Review
Authors: Ortiz-Mallasén Víctor , Claramonte-Gual Eloy , González-Chordá Víctor Manuel , Llagostera-Reverter Irene , Valero-Chillerón María Jesús , Cervera-Gasch Águeda
Published: January 2024

Background: Active aging is considered one of the most effective methods for a healthy aging process. There are numerous clinical practice guidelines that address this model and propose multiple strategies for its achievement through the improvement of motor and cognitive function. Virtual reality is emerging as a potential tool, with various modalities focused on promoting good health maintenance in older adults. The objectives of this review were to map the potential benefits of virtual reality for active aging and delve into adaptability and adherence in older individuals. Methods: A scoping review was conducted on studies published between 2013 and 2023 in English, Spanish, or Catalan, examining virtual reality interventions in older adults. The search was performed using the Medline, CINAHL, Scopus, and Web of Science databases. The methodological quality was assessed using CASPe and FLC 3.0 critical appraisal guidelines. The graphical data were reported narratively, grouping results based on the study characteristics and the impact of virtual reality. Results: The review process resulted in the inclusion of 22 articles out of the initial 459 following the application of the selection criteria. Most articles were randomized controlled trials (45.4%; n = 10), systematic reviews (40.9%; n = 9), observational studies (9%; n = 2), and pilot studies (4.5%; n = 1). The information was organized based on the virtual reality modality (immersive, non-immersive, and 360) and application area (motor, cognitive, and mental health). Conclusions: Virtual reality (both immersive and non-immersive) is a valuable tool for promoting physical exercise in older adults, helping to prevent recurrent accidental falls. It also yields positive results for cognitive stimulation in healthy older individuals, improving memory, depression, and mental health in those with cognitive impairment. Virtual reality is generally well-received by older adults, achieving high adherence rates.
Chemical Analyses of Volatiles from Kumquat Species Grown in Greece—A Study of Antimicrobial Activity
Authors: Ziogas Vasileios , Ganos Christos , Graikou Konstantia , Cheilari Antigoni , Chinou Ioanna
Published: January 2024

The volatiles of different aerial parts of three kumquat species (Fortunella margarita Swingle–Nagami, Fortunella japonica Swingle–Marumi, and Fortunella crassifolia Swingle–Meiwa) growing in Greece were analyzed via GC-MS and evaluated for their antimicrobial properties against nine human pathogenic microorganisms. A total number of 23 compounds were identified in the peel, 38 in the leaves, and 30 in the flowers of the examined species. Limonene was the dominant metabolite in the peels of all three species, germacrene-D was present in the leaves of Nagami and Marumi kumquats, while limonene was the most abundant in the flower of Marumi and Meiwa kumquat but with significant differences in the composition of the total fracture of the essential oil, since compounds with high antimicrobial activity were only present in the flower of Meiwa kumquat. The essential oils from the leaf and peel of the three kumquat species were either inactive or showed weak antimicrobial activity, respectively, against Gram-positive and Gram-negative bacterial strains and pathogenic fungi. Only the essential oil from the flower of F. crassifolia Swingle (Meiwa) showed a stronger effect (MIC values 3.5–7.48 mg/mL) against all the assayed microorganisms. Furthermore, through multivariate statistical analysis, we studied the relationships between the samples regarding their origin (species and plant part), as well as between the chemical composition of the corresponding essential oils and their antimicrobial activity. Considering its chemical profile and antimicrobial activity, the Greek Meiwa flowers’ essential oil seemed a promising essential oil for further exploitation in the food and/or medicinal industry.
The Use of Copper-Based Delafossite to Improve Hydrogen Production Performance: A Review
Authors: Chfii Hasnae , Bouich Amal , Soucase Bernabé Mari
Published: January 2024

This review paper reports on the use of Delafossite as a layer between perovskite-based solar cells to improve hydrogen production efficiency and make the process easier. The investigation delves into the possible breakthroughs in sustainable energy generation by investigating the synergistic interplay between Delafossite and solar technology. This investigation covers copper-based Delafossite material’s properties, influence on cell performance, and function in the electrolysis process for hydrogen production. Some reports investigate the synthesis and characterizations of delafossite materials and try to improve their performance using photo electrochemistry. This work sheds light on the exciting prospects of Delafossite integration using experimental and analytical methodologies.
Annurca Apple Oleolite as Functional Ingredient for the Formulation of Cosmetics with Skin-Antiaging Activity
Authors: Di Lorenzo Ritamaria , Maisto Maria , Ricci Lucia , Piccolo Vincenzo , Marzocchi Adua , Greco Giovanni , Tenore Gian Carlo , Laneri Sonia
Published: January 2024

The identification of natural remedies for the management of the skin aging process is an increasingly growing issue. In this context, ursolic acid (UA), a ubiquitous molecule, mainly contained in Annurca apple (AA) fruit, has demonstrated valuable cosmetic potential. To this end, in the current study, the AA oleolite (AAO, extract in sunflower oil containing 784.40 ± 7.579 µg/mL of UA) was evaluated to inhibit porcine elastase enzymatic reactions through a validated spectrophotometric method. AAO has shown a valuable capacity to contrast the elastase enzyme with a calculated IC50 of 212.76 mg/mL, in comparison to UA (IC50 of 135.24 μg/mL) pure molecules and quercetin (IC50 of 72.47 μg/mL) which are used as positive controls. In this context and in view of the valuable antioxidant potential of AAO, its topical formulation with 2.5% (w/w) AAO was tested in a placebo-controlled, double-blind, two-arm clinical study on 40 volunteers. Our results indicated that after 28 days of treatment, a significant reduction of the nasolabial fold (−7.2 vs. baseline T0, p < 0.001) and forehead wrinkles (−5.3 vs. baseline T0, p < 0.001) were registered in combination with a valuable improvement of the viscoelastic skin parameters, where skin pliability/firmness (R0) and gross elasticity (R2) were significantly ameliorated (−13% vs. baseline T0, p < 0.001 for R0 and +12% vs. baseline T0, p < 0.001 for R2). Finally, considering the positive correlation between skin elasticity and hydration, the skin moisture was evaluated through the estimation of Trans epidermal water loss (TEWL) and skin conductance.
FOXM1 Participates in Trophoblast Migration and Early Trophoblast Invasion: Potential Role in Blastocyst Implantation
Authors: Peñailillo Reyna , Velásquez Victoria , Acuña-Gallardo Stephanie , García Felipe , Sánchez Mario , Nardocci Gino , Illanes Sebastián E. , Monteiro Lara J.
Published: January 2024

Successful implantation requires coordinated migration and invasion of trophoblast cells into a receptive endometrium. Reduced forkhead box M1 (FOXM1) expression limits trophoblast migration and angiogenesis in choriocarcinoma cell lines, and in a rat model, placental FOXM1 protein expression was significantly upregulated in the early stages of pregnancy compared to term pregnancy. However, the precise role of FOXM1 in implantation events remains unknown. By analyzing mice blastocysts at embryonic day (E3.5), we have demonstrated that FOXM1 is expressed as early as the blastocyst stage, and it is expressed in the trophectoderm of the blastocyst. Since controlled oxygen tension is determinant for achieving normal implantation and placentation and a chronic hypoxic environment leads to shallow trophoblast invasion, we evaluated if FOXM1 expression changes in response to different oxygen tensions in the HTR-8/SVneo first trimester human trophoblast cell line and observed that FOXM1 expression was significantly higher when trophoblast cells were cultured at 3% O2, which coincides with oxygen concentrations in the uteroplacental interface at the time of implantation. Conversely, FOXM1 expression diminished in response to 1% O2 that resembles a hypoxic environment in utero. Migration and angiogenesis were assessed following FOXM1 knockdown and overexpression at 3% O2 and 1% O2, respectively, in HTR-8/SVneo cells. FOXM1 overexpression increased transmigration ability and tubule formation. Using a 3D trophoblast invasion model with trophospheres from HTR-8/SVneo cells cultured on a layer of MATRIGEL and of mesenchymal stem cells isolated from menstrual fluid, we observed that trophospheres obtained from 3D trophoblast invasion displayed higher FOXM1 expression compared with pre-invasion trophospheres. Moreover, we have also observed that FOXM1-overexpressing trophospheres increased trophoblast invasion compared with controls. HTR-8/SVneo-FOXM1-depleted cells led to a downregulation of PLK4, VEGF, and MMP2 mRNA expression. Our current findings suggest that FOXM1 participates in embryo implantation by contributing to trophoblast migration and early trophoblast invasion, by inducing transcription activation of genes involved in these processes. Maternal-fetal communication is crucial for trophoblast invasion, and maternal stromal cells may induce higher levels of FOXM1 in trophoblast cells.
Microbiota–Gastric Cancer Interactions and the Potential Influence of Nutritional Therapies
Authors: Raoul Pauline , Maccauro Valeria , Cintoni Marco , Scarpellini Emidio , Ianiro Gianluca , Gasbarrini Antonio , Mele Maria Cristina , Rinninella Emanuele
Published: January 2024

Gastric cancer (GC) is one of the most common causes of cancer deaths, and GC treatments represent a large area of research. Although initially regarded as a sterile organ and unsuitable for microbial communities, the discovery of Helicobacter pylori made us realize that some microbes can colonize the stomach. In recent years, growing interest in gastric bacteria has expanded to the gut microbiota and, more recently, to the oral microbiota. Indeed, the oral–gastric–gut microbiota axis may play a crucial role in maintaining homeostasis, while changes in microbiota composition in GC patients can influence clinical outcomes. On the one hand, the microbiota and its metabolites may significantly influence the progression of GC, while anti-GC treatments such as gastrectomy and chemotherapy may significantly impact the oral–gastric–gut microbiota axis of GC patients. In this context, the role of nutritional therapies, including diet, prebiotics, and probiotics, in treating GC should not be underestimated. Wit this review, we aim to highlight the main role of the gastric, oral, and gut microbiota in GC onset and progression, representing potential future biomarkers for early GC detection and a target for efficient nutritional therapies during the course of GC.
New Insights into the Pro-Inflammatory and Osteoclastogenic Profile of Circulating Monocytes in Osteoarthritis Patients
Authors: Guillem-Llobat Paloma , Marín Marta , Rouleau Matthieu , Silvestre Antonio , Blin-Wakkach Claudine , Ferrándiz María Luisa , Guillén María Isabel , Ibáñez Lidia
Published: January 2024

Osteoarthritis (OA) is a degenerative condition of the articular cartilage with chronic low-grade inflammation. Monocytes have a fundamental role in the progression of OA, given their implication in inflammatory responses and their capacity to differentiate into bone-resorbing osteoclasts (OCLs). This observational–experimental study attempted to better understand the molecular pathogenesis of OA through the examination of osteoclast progenitor (OCP) cells from both OA patients and healthy individuals (25 OA patients and healthy samples). The expression of osteoclastogenic and inflammatory genes was analyzed using RT-PCR. The OA monocytes expressed significantly higher levels of CD16, CD115, TLR2, Mincle, Dentin-1, and CCR2 mRNAs. Moreover, a flow cytometry analysis showed a significantly higher surface expression of the CD16 and CD115 receptors in OA vs. healthy monocytes, as well as a difference in the distribution of monocyte subsets. Additionally, the OA monocytes showed a greater osteoclast differentiation capacity and an enhanced response to an inflammatory stimulus. The results of this study demonstrate the existence of significant differences between the OCPs of OA patients and those of healthy subjects. These differences could contribute to a greater understanding of the molecular pathogenesis of OA and to the identification of new biomarkers and potential drug targets for OA.
Hyperthermia and Photocatalytic Performance of Magnetic Polyvinyl Alcohol under External Magnetic Field
Authors: Khowdiary Manal M. , Alsnani Hind , Darwish Mohamed S. A.
Published: January 2024

The promising physical and chemical properties of components of magnetic polymers could enable extending their intelligent behaviors to material applications. Indeed, investigation into magnetic nanofillers to ensure their uniform dispersion within the polymer matrix remains a great challenge at present. In this work, polyvinyl alcohol-stabilized iron oxide nanoparticles (PVA@IONPs) were prepared using ultrasonic-assisted coprecipitation at room temperature. It is possible to produce PVA@IONPs with desirable shapes and sizes, which would enable the control of their hyperthermia and photocatalytic performance under an external magnetic field. The saturation magnetization of PVA@IONPs (45.08 emu g−1) was enhanced to the level of IONPs (41.93 emu g−1). The PVA@IONPs showed good photocatalytic and outstanding self-heating behavior. The hydrogen yield was 60 mmole min−1 g−1 for photocatalyst PVA@IONPs under visible light with magnetic force. In addition, the PVA@IONPs exhibited a higher specific absorption rate (SAR) than IONPs under the same magnetic field conditions. The PVA@IONPs displayed superior self-heating and photocatalytic performances, rendering them appropriate materials for biomedical and environmental applications.
Exercise Testing in Patients with Pulmonary Hypertension
Authors: Vaidy Anika , Vahdatpour Cyrus A. , Mazurek Jeremy
Published: January 2024

Pulmonary hypertension (PH), defined by a mean pulmonary artery pressure of >20 mm Hg, often presents with non-specific symptoms such as dyspnea and exercise intolerance, making it difficult to diagnose early before the onset of right heart dysfunction. Therefore, exercise testing can be of great utility for clinicians who are evaluating patients with an unclear etiology of exercise intolerance by helping identify the underlying mechanisms of their disease. The presence of PH is associated with adverse clinical outcomes, with distinct differences and patterns in the cardiovascular and ventilatory responses to exercise across various PH phenotypes. We discuss the role of exercise-invasive hemodynamic testing, cardiopulmonary exercise testing, and exercise stress echocardiography modalities across the spectrum of PH.
Acute Effects of 30 g Cyclodextrin Intake during CrossFit® Training on Performance and Fatigue
Authors: Grijota Franscisco Javier , Toro-Román Víctor , Bartolomé Ignacio , Cordero-Román Elías , López Cristian Sánchez , Jiménez Jose Miguel , Martínez-Guardado Ismael
Published: January 2024

The main objective of this study was to investigate the influence of carbohydrate intake (cyclodextrin) on performance during the performance of two consecutive workouts of the day (WODs) lasting 20 min each. Twenty-one male CrossFit (CF) athletes (29.5 ± 4.3 years; 72.81 ± 12.85 kg; 1.74 ± 0.06 m; 3.41 ± 1.21 years of experiences) participated in a crossover, randomized, and double-blind study. The effect of supplementation with 30 g of cyclodextrin (SG) (Cluster Dextrin®) or placebo (PG) (Bolero Advanced Hydration®) was evaluated on the performance of two specific WOD. Additionally, the effect on handgrip maximum strength, countermovement jump (CMJ), Wingate test, and 1 RM bench press test was evaluated. The effect on blood glucose and lactate was also evaluated. No differences were found in time, height, and power (W/Kg) in CMJ. However, there was a percentage improvement in CMJ jump power (W) (p < 0.05) between the groups, assuming an improvement in performance due to the intervention. Moreover, both conditions experimented differences in execution speed between sets (p < 0.05) in pre-WOD, and differences in post-WOD only in the placebo group, as well as decreases in this variable per repetition across the set (p < 0.01) in both conditions. However, no differences were found in the rest of the variables. Supplementation with 30 g of cyclodextrin did not have any metabolic or performance effects in CF tests. Although some differences between groups were observed in CMJ and power tests for bench press, the data are not conclusive and further research is needed in this regard.
Distribution of Suitable Habitats for Soft Corals (Alcyonacea) Based on Machine Learning
Authors: Dong Minxing , Yang Jichao , Fu Yushan , Fu Tengfei , Zhao Qing , Zhang Xuelei , Xu Qinzeng , Zhang Wenquan
Published: January 2024

The soft coral order Alcyonacea is a common coral found in the deep sea and plays a crucial role in the deep-sea ecosystem. This study aims to predict the distribution of Alcyonacea in the western Pacific Ocean using four machine learning-based species distribution models. The performance of these models is also evaluated. The results indicate a high consistency among the prediction results of the different models. The soft coral order is primarily distributed in the Thousand Islands Basin, Japan Trench, and Thousand Islands Trench. Water depth and silicate content are identified as important environmental factors influencing the distribution of Alcyonacea. The RF, Maxent, and XGBoost models demonstrate high accuracies, with the RF model exhibiting the highest prediction accuracy. However, the Maxent model outperforms the other three models in data processing. Developing a high-resolution, high-accuracy, and high-precision habitat suitability model for soft corals can provide a scientific basis and reference for China’s exploration and research in the deep sea field and aid in the planning of protected areas in the high seas.
A Unified Approach to Modeling and Simulation of Underwater Vehicle Multi-Manipulator Systems
Authors: León-González Gloria , Núñez-Cruz Rafael Stanley , Antonio-Yañez Elba Dolores , Herrera-Vidal Juan , Canales-Gómez Giovanni , Rueda-Germán Clementina
Published: January 2024

In this article, the model of a family of underwater vehicle multi-manipulator systems (UVMMS) is obtained by considering all its elements as parts of a unique system, the model includes the forces produced on the manipulators by the movement of the vehicle, as well as the reaction forces on the vehicle produced by the movement of the manipulators. The modeling process is completed using the Newton–Euler approach through the mobile arborescent kinematic chain. This work also presents different approaches to the use of numerical implementations of the proposed model, and simulation results are included to demonstrate that the model is capable to represent the interaction between the vehicle and the manipulators. The proposed model and simulations are important because they allow the design of control strategies that consider all the elements of the system instead of neglecting the interaction forces or considering the vehicle and the arms as uncoupled elements.
Refinements and Applications of Hermite–Hadamard-Type Inequalities Using Hadamard Fractional Integral Operators and GA-Convexity
Authors: Amer Latif Muhammad
Published: January 2024

In this paper, several applications of the Hermite–Hadamard inequality for fractional integrals using GA-convexity are discussed, including some new refinements and similar extensions, as well as several applications in the Gamma and incomplete Gamma functions.
A Matrix-Multiplicative Solution for Multi-Dimensional QBD Processes
Authors: Naumov Valeriy
Published: January 2024

We consider an irreducible positive-recurrent discrete-time Markov process on the state space X=ℤ+M×J, where ℤ+ is the set of non-negative integers and J={1,2,…,n}. The number of states in J may be either finite or infinite. We assume that the process is a homogeneous quasi-birth-and-death process (QBD). It means that the one-step transition probability between non-boundary states (k,i) and (n,j) may depend on i,j, and n−k but not on the specific values of k and n. It is shown that the stationary probability vector of the process is expressed through square matrices of order n, which are the minimal non-negative solutions to nonlinear matrix equations.
Synchronization for Reaction–Diffusion Switched Delayed Feedback Epidemic Systems via Impulsive Control
Authors: Rao Ruofeng , Zhu Quanxin
Published: January 2024

Due to the facts that epidemic-related parameters vary significantly in different stages of infectious diseases and are relatively stable within the same stage, infectious disease models should be switch-type models. However, research on switch-type infectious disease models is scarce due to the complexity and intricate design of switching rules. This scarcity has motivated the writing of this paper. By assuming that switching instants and impulse times occur at different moments, this paper proposes switch rules suitable for impulse control and derives synchronization criteria for reaction–diffusion switch-type infectious disease systems under impulse control. The effectiveness of this method is validated through numerical simulations. It is important to mention that, based on the information available to us, this paper is currently the sole study focusing on switch-type reaction–diffusion models for infectious diseases.
Prognostic Significance of Multifactorial Analysis in Complex Cervical Aero-Digestive Trauma Cases
Authors: Severin Florentina , Danila Radu , Nicolau Andrei , Alexa Anisia Iuliana , Olariu Raluca , Roșca Ștefan , Palade Octavian Dragos , Mocanu Florin , Cobzeanu Mihail Dan , Cobzeanu Bogdan Mihail
Published: January 2024

Background and Objectives: In the context of complex aerodigestive cervical traumas, the prognosis and outcome heavily depend on risk factors, particularly injuries to the larynx, trachea, major digestive tissues, cervical vertebrae, and vascular structures. With the increasing prevalence of trauma as a public health concern, there is a pressing need for epidemiological research and the implementation of preventative measures. The purpose of this research is to establish the profile of the predictable impact factors that determine the prognosis of patients with complex cervical trauma. Methods and Methods: The study group consisted of 106 patients with complex cervical trauma pathology developed by various mechanisms such as car accidents, home-related accidents, aggression, gunshot wounds, and self-inflicted attempts, resulting in hospitalization in the E.N.T. Clinic at “St. Spiridon” Iași Hospital, from 2012 to 2016; medical records were the source of the collected data. Results: Hemodynamic instability upon admission associated with age, muscle and laryngeal injuries, and anemia were identified as negative prognostic factors. Additionally, the utilization of imaging-based paraclinical investigations for diagnosing traumatic lesions emerged as a positive prognostic factor in managing this pathology. The management of penetrating cervical trauma remains a subject of debate, with some advocating for surgical exploration beyond the platysma layer in all cases, while others argue for a more selective conservative approach due to a high rate of negative explorations. Conclusions: The statistical evaluation of epidemiological, clinical, lesion, paraclinical, and therapeutic parameters is needed to establish predictable risk factors in the prognosis of complex aerodigestive cervical trauma.
Investigation of Staphylococcus aureus Biofilm-Associated Toxin as a Potential Squamous Cell Carcinoma Therapeutic
Authors: Ong Zi Xin , Kannan Bavani , Phillips Anthony R. J. , Becker David L.
Published: January 2024

Cancer therapies developed using bacteria and their components have been around since the 19th century. Compared to traditional cancer treatments, the use of bacteria-derived compounds as cancer therapeutics could offer a higher degree of specificity, with minimal off-target effects. Here, we explored the use of soluble bacteria-derived toxins as a potential squamous cell carcinoma (SCC) therapeutic. We optimized a protocol to generate Staphylococcus aureus biofilm-conditioned media (BCM), where soluble bacterial products enriched in the development of biofilms were isolated from a bacterial culture and applied to SCC cell lines. Bioactive components of S. aureus ATCC 29213 (SA29213) BCM display selective toxicity towards cancerous human skin SCC-12 at low doses, while non-cancerous human keratinocyte HaCaT and fibroblast BJ-5ta are minimally affected. SA29213 BCM treatment causes DNA damage to SCC-12 and initiates Caspase 3-dependent-regulated cell death. The use of the novel SA29213 bursa aurealis transposon mutant library led to the identification of S. aureus alpha hemolysin as the main bioactive compound responsible for the observed SCC-12-specific toxicity. The antibody neutralisation of Hla eradicates the cytotoxicity of SA29213 BCM towards SCC-12. Hla displays high SCC-12-specific toxicity, which is exerted primarily through Hla-ADAM10 interaction, Hla oligomerisation, and pore formation. The high target specificity and potential to cause cell death in a controlled manner highlight SA29213 Hla as a good candidate as an alternative SCC therapeutic.
Genome Sequencing and Characterization of Bacillus velezensis N23 as Biocontrol Agent against Plant Pathogens
Authors: Yang Panlei , Zeng Qingchao , Jiang Wenxiao , Wang Luotao , Zhang Jie , Wang Zhenshuo , Wang Qi , Li Yan
Published: January 2024

The overuse of chemical fungicides against fungal pathogens adversely affects soil and plant health, resulting in environmental problems and food safety. Therefore, biocontrol is considered as an environmentally friendly and cost-effective green technique in environmental protection and agricultural production. We obtained a bacterial strain N23 from a contaminated plate which showed significant inhibition to anthracnose. The strain N23 was identified as Bacillus velezensis based on 16S rRNA gene, gyrA gene, and whole-genome sequence. The bacterium N23 was able to suppress the mycelial growth of numerous plant pathogenic fungi on solid media. Tomato seeds treated with strain N23 showed significantly higher germination levels than untreated ones. Moreover, strain N23 effectively reduced the lesion area of pepper anthracnose disease in planta. The gene clusters responsible for antifungal metabolites (fengycin, surfactin, and iturin) were identified in the genome sequence of N23 based on genome mining and PCR. Furthermore, methanol extracts of the bacterial culture caused significant inhibition in growth of the fungal Colletotrichum sp. and Botrytis cinerea. These findings suggested that B. velezensis N23 could be a potential biocontrol agent in agricultural production and a source of antimicrobial compounds for further exploitation.
A Mine Closure Risk Rating System for South Africa
Authors: Cole Megan J.
Published: January 2024

Mine closure is a growing concern in mining countries around the world due to the associated environmental and social impacts. This is particularly true in developing countries like South Africa where poverty, social deprivation and unemployment are widespread and environmental governance is not strong. South Africa has 230 operating mines located in diverse natural and social settings. Over 6 million people live in urban and rural mining host communities who will be significantly affected by mine closure. The national, provincial and local governments need guidance in identifying high-risk areas and relevant policy and programmatic interventions. This paper describes the development of a quantitative mine closure risk rating system that assesses the likelihood of mine closure, the risk of social impact and the risk of environmental impact of mine closure for every operating mine in the country. The paper visualises the high likelihood of closure and environmental impacts for numerous coal and gold mines, and the significant social risks in the deprived rural platinum and chrome mining areas. The rating system was tested with 10 mines and 19 experts, and the resulting maps are communicated in an online South African Mine Closure Risk and Opportunity Atlas. The risk ratings could be used in mine closure planning and management by mining companies, consultancies, governments and affected communities. While this risk rating system has been designed for South Africa, the methodology and framework could be applied to any mining country in the world.
Experimental and Computational Analysis of Synthesis Conditions of Hybrid Nanoflowers for Lipase Immobilization
Authors: Souza Danivia Endi S. , Santos Lucas M. F. , Freitas João P. A. , Almeida Lays C. de , Santos Jefferson C. B. , Souza Ranyere Lucena de , Pereira Matheus M. , Lima Álvaro S. , Soares Cleide M. F.
Published: January 2024

This work presents a framework for evaluating hybrid nanoflowers using Burkholderia cepacia lipase. It was expanded on previous findings by testing lipase hybrid nanoflowers (hNF-lipase) formation over a wide range of pH values (5–9) and buffer concentrations (10–100 mM). The free enzyme activity was compared with that of hNF-lipase. The analysis, performed by molecular docking, described the effect of lipase interaction with copper ions. The morphological characterization of hNF-lipase was performed using scanning electron microscopy. Fourier Transform Infrared Spectroscopy performed the physical–chemical characterization. The results show that all hNF-lipase activity presented values higher than that of the free enzyme. Activity is higher at pH 7.4 and has the highest buffer concentration of 100 mM. Molecular docking analysis has been used to understand the effect of enzyme protonation on hNF-lipase formation and identify the main the main binding sites of the enzyme with copper ions. The hNF-lipase nanostructures show the shape of flowers in their micrographs from pH 6 to 8. The spectra of the nanoflowers present peaks typical of the amide regions I and II, current in lipase, and areas with P–O vibrations, confirming the presence of the phosphate group. Therefore, hNF-lipase is an efficient biocatalyst with increased catalytic activity, good nanostructure formation, and improved stability.
A Fine Analysis of Zn Species Structure and Distribution in Zn/ZSM-5 Catalysts by Linear Combination Fitting Analysis of XANES Spectra
Authors: Li Baichao , Gao Jie , Shao Jiabei , Geng Rui , Qin Zhangfeng , Wang Jianguo , Fan Weibin , Dong Mei
Published: January 2024

Investigating the distribution of different Zn species on Zn-containing zeolite catalysts is crucial for identifying the active sites and establishing the relationship between the catalyst’s structure and its activity in the process of ethylene aromatization. By utilizing X-ray absorption near edge spectra (XANES) of various reference samples, this study employed linear combination fitting (LCF) analysis on XANES spectra of real samples to accurately measure the changes in the distribution of Zn species in Zn-containing HZSM-5 zeolites under different Zn sources and loadings. The results showed that ZnOH+, ZnO clusters, and ZnO crystalline structures coexist in Zn/HZSM-5 catalysts prepared through physical mixing and incipient wet impregnation methods. A similar trend was observed for catalysts prepared using different methods, with an increase in Zn content resulting in a decrease in the proportion of ZnOH+ and a significant increase in the amount of larger ZnO crystals. Furthermore, ZnO clusters were confined within the zeolite pores. The findings of this study established a direct correlation between the amount of ZnOH+ determined through LCF analysis and both the rate of hydrogen production and the rate of aromatics formation, providing strong evidence for the catalytic role of ZnOH+ as an active center for dehydrogenation, which plays a key role in promoting the formation of aromatics. The method of LCF analysis on XANES spectra allows for the determination of the local structure of Zn species, facilitating a more precise analysis based on the distribution of these species. This method not only provides detailed information about the Zn species but also enhances the accuracy of the overall analysis.
Light-Responsive and Dual-Targeting Liposomes: From Mechanisms to Targeting Strategies
Authors: Agiba Ahmed M. , Arreola-Ramírez José Luis , Carbajal Verónica , Segura-Medina Patricia
Published: January 2024

In recent years, nanocarriers have played an ever-increasing role in clinical and biomedical applications owing to their unique physicochemical properties and surface functionalities. Lately, much effort has been directed towards the development of smart, stimuli-responsive nanocarriers that are capable of releasing their cargos in response to specific stimuli. These intelligent-responsive nanocarriers can be further surface-functionalized so as to achieve active tumor targeting in a sequential manner, which can be simply modulated by the stimuli. By applying this methodological approach, these intelligent-responsive nanocarriers can be directed to different target-specific organs, tissues, or cells and exhibit on-demand controlled drug release that may enhance therapeutic effectiveness and reduce systemic toxicity. Light, an external stimulus, is one of the most promising triggers for use in nanomedicine to stimulate on-demand drug release from nanocarriers. Light-triggered drug release can be achieved through light irradiation at different wavelengths, either in the UV, visible, or even NIR region, depending on the photophysical properties of the photo-responsive molecule embedded in the nanocarrier system, the structural characteristics, and the material composition of the nanocarrier system. In this review, we highlighted the emerging functional role of light in nanocarriers, with an emphasis on light-responsive liposomes and dual-targeted stimuli-responsive liposomes. Moreover, we provided the most up-to-date photo-triggered targeting strategies and mechanisms of light-triggered drug release from liposomes and NIR-responsive nanocarriers. Lastly, we addressed the current challenges, advances, and future perspectives for the deployment of light-responsive liposomes in targeted drug delivery and therapy.
An Automated Micro Solid-Phase Extraction (μSPE) Liquid Chromatography-Mass Spectrometry Method for Cyclophosphamide and Iphosphamide: Biological Monitoring in Antineoplastic Drug (AD) Occupational Exposure
Authors: Dugheri Stefano , Squillaci Donato , Saccomando Valentina , Marrubini Giorgio , Bucaletti Elisabetta , Rapi Ilaria , Fanfani Niccolò , Cappelli Giovanni , Mucci Nicola
Published: January 2024

Despite the considerable steps taken in the last decade in the context of antineoplastic drug (AD) handling procedures, their mutagenic effect still poses a threat to healthcare personnel actively involved in compounding and administration units. Biological monitoring procedures usually require large volumes of sample and extraction solvents, or do not provide adequate sensitivity. It is here proposed a fast and automated method to evaluate the urinary levels of cyclophosphamide and iphosphamide, composed of a miniaturized solid phase extraction (µSPE) followed by ultrahigh-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) analysis. The extraction procedure, developed through design of experiments (DoE) on the ePrep One Workstation, required a total time of 9.5 min per sample, with recoveries of 77–79% and a solvent consumption lower than 1.5 mL per 1 mL of urine sample. Thanks to the UHPLC-MS/MS method, the limits of quantification (LOQ) obtained were lower than 10 pg/mL. The analytical procedure was successfully applied to 23 urine samples from compounding wards of four Italian hospitals, which resulted in contaminations between 27 and 182 pg/mL.
On the Fragmentation of Ni(II) β-Diketonate-Diamine Complexes as Molecular Precursors for NiO Films: A Theoretical and Experimental Investigation
Authors: Invernizzi Cristiano , Tabacchi Gloria , Seraglia Roberta , Benedet Mattia , Roverso Marco , Maccato Chiara , Bogialli Sara , Barreca Davide , Fois Ettore
Published: January 2024

NiO-based nanomaterials have attracted considerable interest for different applications, which have stimulated the implementation of various synthetic approaches aimed at modulating their chemico-physical properties. In this regard, their bottom-up preparation starting from suitable precursors plays an important role, although a molecular-level insight into their reactivity remains an open issue to be properly tackled. In the present study, we focused on the fragmentation of Ni(II) diketonate-diamine adducts, of interest as vapor-phase precursors for Ni(II) oxide systems, by combining electrospray ionization mass spectrometry (ESI-MS) with multiple collisional experiments (ESI-MSn) and theoretical calculations. The outcomes of this investigation revealed common features in the fragmentation pattern of the target compounds: (i) in the first fragmentation, the three complexes yield analogous base-peak cations by losing a negatively charged diketonate moiety; in these cations, Ni-O and Ni-N interactions are stronger and the Ni positive charge is lower than in the parent neutral complexes; (ii) the tendency of ligand electronic charge to migrate towards Ni further increases in the subsequent fragmentation, leading to the formation of a tetracoordinated Ni environment featuring an interesting cation-π intramolecular interaction.
Terahertz Time-Domain Spectroscopic Characteristics of Typical Metallic Minerals
Authors: Zhang Jingjing , Huang Haochong , Zhao Pengbo , Xu Luyong , Tan Zhenbo , Zhao Jinyuan , Yuan Enhui , Zheng Zhiyuan , Li Shanshan , Li Xinyu , Qiu Kunfeng
Published: January 2024

Accurate identification and understanding of various metallic minerals are crucial for deciphering geological formations, structures, and ages. Giving their pivotal role as essential natural resources, a microscopic exploration of metallic minerals becomes imperative. Traditional analytical methods, while helpful, exhibit certain limitations. However, terahertz time-domain spectroscopy, distinguished by its high signal-to-noise ratio, expansive frequency band, and low incident wave energy, is a promising complement to conventional techniques in characterizing metallic minerals. This study employs terahertz time-domain spectroscopy to examine samples of Stibnite, Sphalerite, Galena, and Pyrite originating from diverse geological conditions. The vibrations of molecules within these metallic minerals induce discernible changes in the terahertz spectra. Our findings untiate the extensive potential of terahertz time-domain spectroscopy in the characterization of metallic minerals, affirming its considerable practical value in mineral resource exploration.
One-Dimensional and Two-Dimensional Zn(II) Coordination Polymers with Ditopic Imidazo[1,5-a]pyridine: A Structural and Computational Study
Authors: Sozzi Mattia , Chierotti Michele R. , Gobetto Roberto , Gomila Rosa M. , Marzaroli Vittoria , Priola Emanuele , Volpi Giorgio , Zago Stefano , Frontera Antonio , Garino Claudio
Published: January 2024

Zn(II) coordination polymers are being increasingly studied for their stability and properties. Similarly, there is a growing interest in imidazo[1,5-a]pyridine derivatives, which show great potential in luminescence and pharmaceutical applications. In this work, we successfully synthesized and crystallized three new coordination polymers, using Zn(II) as the metallic node, dicarboxylic acids of different length and nature as linkers, and a linear ditopic imidazo[1,5-a]pyridine derivative, to explore the role of this molecule as a propagator of the dimensionality of the structure or as an ancillary ligand. Our work demonstrates the structural capability of imidazo[1,5-a]pyridines in an unexplored domain for this family of ligands. Notably, we observed a pronounced ability of this heterocyclic scaffold to establish π···π interactions in the solid state. The supramolecular π-stacked assemblies were theoretically analyzed using DFT calculations based on model structures.
The Role of Neuroglia in Administrating Nerve Blockers and Anesthesia to Patients
Authors: Patel Anjali , Al-Bahou Raja , Thakkar Rajvi , Patel Drashti , Foster Devon , Benjamin Jonathan , Pedreira Marian , Lucke-Wold Brandon
Published: January 2024

Dysfunction of the neuroglia can have profound consequences on the blood–brain barrier (BBB). Studies have shown that the disruption of astrocytic–endothelial interaction can compromise the permeability of BBB and its effectiveness in selectively regulating the exchange of substances. Microglia have recently been recognized to have a significant role in the initiation of chronic pain and in its interactions with various nerve blockers and anesthetic agents. Microglia have a role in pain resolution via a pathway that involves Cannabinoid receptor type 2 activation and MAP kinase phosphorylation. Understanding the role of these cells in the context of neuropathic pain and neurological disorders can aid in improving clinical outcomes and the challenging nature of managing pain. Advancing studies have proposed pharmacological and genetic modulation of microglia as a potential treatment option for patients with chronic pain.