bluuebunny/crossref_metadata_2025 huggingface dataset:
Erythrophagocytosis by Microglia/Macrophage in Intracerebral Hemorrhage: From Mechanisms to Translation
Authors: Liu Jiaxin , Zhu Zhiyuan , Leung Gilberto Ka-Kit
Published: February 2022

Intracerebral hemorrhage (ICH) is a devastating condition characterized by hematoma related mass effect. Microglia/macrophage (M φ) are rapidly recruited in order to remove the red blood cells through erythrophagocytosis. Efficient erythrophagocytosis can detoxify hemolytic products and facilitate neurological recovery after ICH. The underlying mechanisms include modulation of inflammatory response and oxidative stress, among others. It is a dynamic process mediated by a cascade of signal transduction, including “find-me” signals, “eat-me” signals and a set of phagocytotic receptors-ligand pairs that may be exploited as therapeutic targets. This review summarizes mechanistic signaling pathways of erythrophagocytosis and highlights the potential of harnessing M φ-mediated phagocytosis for ICH treatment.
Neural Signature of Buying Decisions in Real-World Online Shopping Scenarios – An Exploratory Electroencephalography Study Series
Authors: Horr Ninja K. , Han Keren , Mousavi Bijan , Tang Ruihong
Published: February 2022

The neural underpinnings of decision-making are critical to understanding and predicting human behavior. However, findings from decision neuroscience are limited in their practical applicability due to the gap between experimental decision-making paradigms and real-world choices. The present manuscript investigates the neural markers of buying decisions in a fully natural purchase setting: participants are asked to use their favorite online shopping applications to buy common goods they are currently in need of. Their electroencephalography (EEG) is recorded while they view the product page for each item. EEG responses to pages for products that are eventually bought are compared to those that are discarded. Study 1 repeats this procedure in three batches with different participants, product types, and time periods. In an explorative analysis, two neural markers for buying compared to no-buying decisions are discovered over all three batches: frontal alpha asymmetry peak and frontal theta power peak. Occipital alpha power at alpha asymmetry peaks differs in only one of the three batches. No further significant markers are found. Study 2 compares the natural product search to a design in which subjects are told which product pages to view. In both settings, the frontal alpha asymmetry peak is increased for buying decisions. Frontal theta peak increase is replicated only when subjects search through product pages by themselves. The present study series represents an attempt to find neural markers of real-world decisions in a fully natural environment and explore how those markers can change due to small adjustments for the sake of experimental control. Limitations and practical applicability of the real-world approach to studying decision-making are discussed.
Microstate Detection in Naturalistic Electroencephalography Data: A Systematic Comparison of Topographical Clustering Strategies on an Emotional Database
Authors: Hu Wanrou , Zhang Zhiguo , Zhang Li , Huang Gan , Li Linling , Liang Zhen
Published: February 2022

Electroencephalography (EEG) microstate analysis is a powerful tool to study the spatial and temporal dynamics of human brain activity, through analyzing the quasi-stable states in EEG signals. However, current studies mainly focus on rest-state EEG recordings, microstate analysis for the recording of EEG signals during naturalistic tasks is limited. It remains an open question whether current topographical clustering strategies for rest-state microstate analysis could be directly applied to task-state EEG data under the natural and dynamic conditions and whether stable and reliable results could still be achieved. It is necessary to answer the question and explore whether the topographical clustering strategies would affect the performance of microstate detection in task-state EEG microstate analysis. If it exists differences in microstate detection performance when different topographical clustering strategies are adopted, then we want to know how the alternations of the topographical clustering strategies are associated with the naturalistic task. To answer these questions, we work on a public emotion database using naturalistic and dynamic music videos as the stimulation to evaluate the effects of different topographical clustering strategies for task-state EEG microstate analysis. The performance results are systematically examined and compared in terms of microstate quality, task efficacy, and computational efficiency, and the impact of topographical clustering strategies on microstate analysis for naturalistic task data is discussed. The results reveal that a single-trial-based bottom-up topographical clustering strategy (bottom-up) achieves comparable results with the task-driven-based top-down topographical clustering (top-down). It suggests that, when task information is unknown, the single-trial-based topographical clustering could be a good choice for microstate analysis and neural activity study on naturalistic EEG data.
Nutritional Screening and Anthropometry in Patients Admitted From the Emergency Department
Authors: Speranza Enza , Santarpia Lidia , Marra Maurizio , Di Vincenzo Olivia , Naccarato Marianna , De Caprio Carmela , Morlino Delia , D'Onofrio Gaetano , Contaldo Franco , Pasanisi Fabrizio
Published: February 2022

Background

Due to the high prevalence of malnutrition among hospitalized patients, screening and assessment of nutritional status should be routinely performed upon hospital admission. The main objective of this observational study was to evaluate the prevalence of and the risk for malnutrition, as identified by using three nutritional screening tests, and to observe whether some anthropometric and functional parameters used for nutritional evaluation were related to these test scores.

Methods

This single-center observational study included 207 patients admitted from the emergency department for hospitalization in either the internal medicine or surgery units of our institution from September 2017 to December 2018. The prevalence of malnutrition among this patient sample was evaluated by using the Nutritional Risk Screening (NRS-2002), the Subjective Global Assessment (SGA) and the Global Leadership Initiative on Malnutrition (GLIM) criteria. Body mass index (BMI), bioimpedance analysis (BIA), handgrip strength (HGS) and calf circumference (CC) assessments were also performed.

Results

According to the NRS-2002, 93% of the patients were at no risk or at low nutritional risk (NRS score < 3), and 7% were at a high nutritional risk (NRS score ≥ 3). On the other hand, according to the SGA, 46.3% of the patients were well-nourished (SGA-a), 49.8% were moderately malnourished (SGA-b), and 3.9% were severely malnourished (SGA-c). Finally, according to the GLIM criteria, 18% patients were malnourished. Body weight, body mass index (BMI), phase angle (PhA), CC and HGS were significantly lower in the patients with NRS scores ≥ 3, SGA-c and in patients with stage 1 and stage 2 malnutrition, according to the GLIM criteria.

Conclusion

The NRS-2002, the SGA and the GLIM criteria appear to be valuable tools for the screening and assessment of nutritional status. In particular, the lowest NRS-2002, SGA and GLIM scores were associated with the lowest PhA and CC. Nevertheless, a weekly re-evaluation of patients with better screening and assessment scores is recommended to facilitate early detection of changes in nutritional status.
Establishment of an Individualized Predictive Model to Reduce the Core Number for Systematic Prostate Biopsy: A Dual Center Study Based on Stratification of the Disease Risk Score
Authors: Chen Zeyu , Qu Min , Shen Xianqi , Jiang Shaoqin , Zhang Wenhui , Ji Jin , Wang Yan , Zhang Jili , Chen Zhenlin , Lin Lu , Li Mengqiang , Wu Cheng , Gao Xu
Published: February 2022

Purpose

To establish an individualized prostate biopsy model that reduces unnecessary biopsy cores based on multiparameter MRI (mpMRI).

Materials and Methods

This retrospective, non-inferiority dual-center study retrospectively included 609 patients from the Changhai Hospital from June 2017 to November 2020 and 431 patients from the Fujian Union Hospital between 2014 and 2019. Clinical, radiological, and pathological data were analyzed. Data from the Changhai Hospital were used for modeling by calculating the patients’ disease risk scores. Data from the Fujian Union Hospital were used for external verification.

Results

Based on the data of 609 patients from the Changhai Hospital, we divided the patients evenly into five layers according to the disease risk score. The area under the receiver operating characteristic (ROC) curve (AUC) with 95% confidence intervals (CI) was analyzed. Twelve-core systemic biopsy (12-SBx) was used as the reference standard. The SBx cores from each layer were reduced to 9, 6, 5, 4, and 4. The data of 279 patients with benign pathological results from the Fujian Union Hospital were incorporated into the model. No patients were in the first layer. The accuracies of the models for the other layers were 88, 96.43, 94.87, and 94.59%. The accuracy of each layer would be increased to 96, 100, 100, and 97.30% if the diagnosis of non-clinically significant prostate cancer was excluded.

Conclusions

In this study, we established an individualized biopsy model using data from a dual center. The results showed great accuracy of the model, indicating its future clinical application.
Circulating Long Non-Coding RNAs LINC00324 and LOC100507053 as Potential Liquid Biopsy Markers for Esophageal Squamous Cell Carcinoma: A Pilot Study
Authors: Sharma Uttam , Barwal Tushar Singh , Khandelwal Akanksha , Rana Manjit Kaur , Rana Amrit Pal Singh , Singh Karuna , Jain Aklank
Published: February 2022

Background

Despite the availability of advanced technology to detect and treat esophageal squamous cell carcinoma (ESCC), the 5-year survival rate of ESCC patients is still meager. Recently, long non-coding RNAs (lncRNAs) have emerged as essential players in the diagnosis and prognosis of various cancers.

Objective

This pilot study focused on identifying circulating lncRNAs as liquid biopsy markers for the ESCC.

Methodology

We performed next-generation sequencing (NGS) to profile circulating lncRNAs in ESCC and healthy individuals’ blood samples. The expression of the top five upregulated and top five downregulated lncRNAs were validated through quantitative real-time PCR (qRT-PCR), including samples used for the NGS. Later, we explored the diagnostic/prognostic potential of lncRNAs and their impact on the clinicopathological parameters of patients. To unravel the molecular target and pathways of identified lncRNAs, we utilized various bioinformatics tools such as lncRnome, RAID v2.0, Starbase, miRDB, TargetScan, Gene Ontology, and KEGG pathways.

Results

Through NGS profiling, we obtained 159 upregulated, 137 downregulated, and 188 neutral lncRNAs in ESCC blood samples compared to healthy individuals. Among dysregulated lncRNAs, we observed LINC00324 significantly upregulated (2.11-fold; p-value = 0.0032) and LOC100507053 significantly downregulated (2.22-fold; p-value = 0.0001) in ESCC patients. Furthermore, we found LINC00324 and LOC100507053 could discriminate ESCC cancer patients’ from non-cancer individuals with higher accuracy of Area Under the ROC Curve (AUC) = 0.627 and 0.668, respectively. The Kaplan-Meier and log-rank analysis revealed higher expression levels of LINC00324 and lower expression levels of LOC100507053 well correlated with the poor prognosis of ESCC patients with a Hazard ratio of LINC00324 = 2.48 (95% CI: 1.055 to 5.835) and Hazard ratio of LOC100507053 = 4.75 (95% CI: 2.098 to 10.76)]. Moreover, we also observed lncRNAs expression well correlated with the age (>50 years), gender (Female), alcohol, tobacco, and hot beverages consumers. Using bioinformatics tools, we saw miR-493-5p as the direct molecular target of LINC00324 and interacted with the MAPK signaling pathway in ESCC pathogenesis.

Conclusion

This pilot study suggests that circulating LINC00324 and LOC100507053 can be used as a liquid biopsy marker of ESCC; however, multicentric studies are still warranted.
Predicting the WHO/ISUP Grade of Clear Cell Renal Cell Carcinoma Through CT-Based Tumoral and Peritumoral Radiomics
Authors: Ma Yanqing , Guan Zheng , Liang Hong , Cao Hanbo
Published: February 2022

Objectives

This study aims to establish predictive logistic models for the World Health Organization/International Society of Urological Pathology (WHO/ISUP) grades of clear cell renal cell carcinoma (ccRCC) based on tumoral and peritumoral radiomics.

Methods

A cohort of 370 patients with pathologically confirmed ccRCCs were included in this retrospective study between January 2014 and December 2020 according to the WHO/ISUP grading system. The volume of interests of triphasic computed tomography images were depicted manually using the “itk-SNAP” software, and the radiomics features were calculated. The cohort was segmented into the training cohort and validation cohort with a random proportion of 7:3. After extraction of radiomics features by analysis of variance (ANOVA) or Mann-Whitney U test, correlation analysis, and the least absolute shrinkage and selection operator (LASSO) method, the logistic models of tumoral radiomics (LR-tumor) and peritumoral radiomics (LR-peritumor) were developed. The LR-peritumor was subdivided into LR-peritumor-2mm, LR-peritumor-5mm, and LR-peritumor-10mm, and the LR-peritumor-2mm was subdivided into LR-peritumor-kid and LR-peritumor-fat based on the neighboring tissues of ccRCCs. Finally, an integrative model of tumoral and peritumoral radiomics (LR-tumor/peritumor) was built. The value of areas under the receiver operator characteristics curve (AUCs) was calculated to assess the efficacy of the models.

Results

There were 209 low-grade and 161 high-grade ccRCCs enrolled. The AUCs of LR-tumor in CT images of venous phase were 0.802 in the training cohort and 0.796 in the validation cohort. The AUCs were higher in the LR-peritumor-2mm than those in LR-peritumor-5mm and LR-peritumor-10mm (training cohort: 0.788 vs. 0.788 and 0.759; validation cohort: 0.787 vs. 0.785 and 0.758). Moreover, the AUCs of LR-peritumor-fat were higher compared with those of LR-peritumor-kid. The LR-tumor/peritumor displayed the highest AUCs of 0.812 in the training cohort and 0.804 in the validation cohort.

Conclusions

The tumoral and peritumoral radiomics helped to predict the WHO/ISUP grades of ccRCCs. On the diagnostic performance of peritumoral radiomics, better results were seen for the LR-peritumor-2mm and LR-peritumor-fat.
Case Report: A BRCA2 Mutation Identified Through Next-Generation Sequencing in a Birt–Hogg–Dubè Syndrome Family
Authors: Bandini Erika , Cangini Ilaria , Arcangeli Valentina , Ravegnani Mila , Andreotti Virginia , Prisinzano Giovanna , Pastorino Lorenza , Martinelli Giovanni , Falcini Fabio , Calistri Daniele , Zampiga Valentina , Danesi Rita
Published: February 2022

Birt–Hogg–Dubé syndrome (BHDS) is a rare autosomal dominant inherited disorder caused by a mutation in folliculin (FLCN) gene transmitted via germline autosomal dominant pattern. Patients with this syndrome have an increased susceptibility to renal cell carcinoma, lung cysts, spontaneous pneumothorax, and benign skin hamartomas, and its diagnosis is not easy and consequently underestimated. Several mutations have been identified in FLCN gene, among which the majority of alterations are frameshift (insertion/deletion), nonsense, or splice-site mutations that generally produce unfunctional truncated FLCN proteins. Our aim is to present a case of a BHDS family whose proband is a 56-year-old patient who has been experiencing multiple disorders, has an FLCN genetic mutation, and has also been identified to have a pathogenic variant in BRCA2 gene. Our further purpose is to emphasize the importance of the next-generation sequencing (NGS) approach to identify potential multiple germline mutations in complex and rare oncologic disorders, allowing strict and more targeted cancer screening programs.
Restorative Effects of Inulin From Codonopsis pilosula on Intestinal Mucosal Immunity, Anti-Inflammatory Activity and Gut Microbiota of Immunosuppressed Mice
Authors: Zou Yuan-Feng , Li Cen-Yu , Fu Yu-Ping , Feng Xin , Peng Xi , Feng Bin , Li Li-Xia , Jia Ren-Yong , Huang Chao , Song Xu , Lv Cheng , Ye Gang , Zhao Ling , Li Yang-Ping , Zhao Xing-Hong , Yin Li-Zi , Yin Zhong-Qiong
Published: February 2022

An inulin (CPPF), isolated from a traditional Chinese herbal medicine Codonopsis pilosula, was characterized and demonstrated with potential prebiotic activity in vitro before. Based on its non-digested feature, the intestinal mucosa and microbiota modulatory effects in vivo on immunosuppressed mice were investigated after oral administration of 200, 100 and 50 mg/kg of CPPF for 7 days. It was demonstrated that the secretions of sIgA and mucin 2 (Muc2) in ileum were improved by CPPF, and the anti-inflammatory activities in different intestine parts were revealed. The intestine before colon could be the target active position of CPPF. As a potential prebiotic substance, a gut microbiota restorative effect was also presented by mainly modulating the relative abundance of Eubacteriales, including Oscillibacter, unidentified Ruminococcus and Lachnospiraceae after high-throughput pyrosequencing of V4 region of 16S rRNA analysis. All these results indicated that this main bioactive ingredient inulin from C. pilosula was a medicinal prebiotic with enhancing mucosal immune, anti-inflammatory and microbiota modulatory activities.
Prescription Rates, Polypharmacy and Prescriber Variability in Swiss General Practice—A Cross-Sectional Database Study
Authors: Rachamin Yael , Jäger Levy , Meier Rahel , Grischott Thomas , Senn Oliver , Burgstaller Jakob M. , Markun Stefan
Published: February 2022

Purpose: The frequency of medication prescribing and polypharmacy has increased in recent years in different settings, including Swiss general practice. We aimed to describe patient age- and sex-specific rates of polypharmacy and of prescriptions of the most frequent medication classes, and to explore practitioner variability in prescribing.

Methods: Retrospective cross-sectional study based on anonymized electronic medical records data of 111 811 adult patients presenting to 116 Swiss general practitioners in 2019. We used mixed-effects regression analyses to assess the association of patient age and sex with polypharmacy (≥5 medications) and with the prescription of specific medication classes (second level of the Anatomical Therapeutic Chemical Classification System). Practitioner variability was quantified in terms of the random effects distributions.

Results: The prevalence of polypharmacy increased with age from 6.4% among patients aged 18–40 years to 19.7% (41–64 years), 45.3% (65–80 years), and 64.6% (81–92 years), and was higher in women than in men, particularly at younger ages. The most frequently prescribed medication classes were antiinflammatory and antirheumatic products (21.6% of patients), agents acting on the renin-angiotensin system (19.9%), analgesics (18.7%), and drugs for acid related disorders (18.3%). Men were more often prescribed agents targeting the cardiovascular system, whereas most other medications were more often prescribed to women. The highest practitioner variabilities were observed for vitamins, for antiinflammatory and antirheumatic products, and for mineral supplements.

Conclusion: Based on practitioner variability, prevalence, and risk potential, antiinflammatory drugs and polypharmacy in older patients appear to be the most pressing issues in current drug prescribing routines.
Identification of Medicinal Bidens Plants for Quality Control Based on Organelle Genomes
Authors: Wu Liwei , Nie Liping , Guo Shiying , Wang Qing , Wu Zhengjun , Lin Yulin , Wang Yu , Li Baoli , Gao Ting , Yao Hui
Published: February 2022

Bidens plants are annuals or perennials of Asteraceae and usually used as medicinal materials in China. They are difficult to identify by using traditional identification methods because they have similar morphologies and chemical components. Universal DNA barcodes also cannot identify Bidens species effectively. This situation seriously hinders the development of medicinal Bidens plants. Therefore, developing an accurate and effective method for identifying medicinal Bidens plants is urgently needed. The present study aims to use phylogenomic approaches based on organelle genomes to address the confusing relationships of medicinal Bidens plants. Illumina sequencing was used to sequence 12 chloroplast and eight mitochondrial genomes of five species and one variety of Bidens. The complete organelle genomes were assembled, annotated and analysed. Phylogenetic trees were constructed on the basis of the organelle genomes and highly variable regions. The organelle genomes of these Bidens species had a conserved gene content and codon usage. The 12 chloroplast genomes of the Bidens species were 150,489 bp to 151,635 bp in length. The lengths of the eight mitochondrial genomes varied from each other. Bioinformatics analysis revealed the presence of 50–71 simple sequence repeats and 46–181 long repeats in the organelle genomes. By combining the results of mVISTA and nucleotide diversity analyses, seven candidate highly variable regions in the chloroplast genomes were screened for species identification and relationship studies. Comparison with the complete mitochondrial genomes and common protein-coding genes shared by each organelle genome revealed that the complete chloroplast genomes had the highest discriminatory power for Bidens species and thus could be used as a super barcode to authenticate Bidens species accurately. In addition, the screened highly variable region trnS-GGA-rps4 could be also used as a potential specific barcode to identify Bidens species.
Long-Term Endurance Exercise Training Alters Repolarization in a New Rabbit Athlete’s Heart Model
Authors: Kui Péter , Polyák Alexandra , Morvay Nikolett , Tiszlavicz László , Nagy Norbert , Ördög Balázs , Takács Hedvig , Leprán István , Farkas András , Papp Julius Gy. , Jost Norbert , Varró András , Baczkó István , Farkas Attila S.
Published: February 2022

In the present study, the effect of long-term exercise training was investigated on myocardial morphological and functional remodeling and on proarrhythmic sensitivity in a rabbit athlete’s heart model. New-Zealand white rabbits were trained during a 12-week long treadmill running protocol and compared with their sedentary controls. At the end of the training protocol, echocardiography, in vivo and in vitro ECG recordings, proarrhythmic sensitivity with dofetilide (nM) were performed in isolated hearts, and action potential duration (APD) measurements at different potassium concentrations (4.5 and 2 mM) were made in the isolated papillary muscles. Expression levels of the slow component of delayed rectifier potassium current and fibrosis synthesis and degradation biomarkers were quantified. Echocardiography showed a significantly dilated left ventricle in the running rabbits. ECG PQ and RR intervals were significantly longer in the exercised group (79 ± 2 vs. 69 ± 2 ms and 325 ± 11 vs. 265 ± 6 ms, p < 0.05, respectively). The in vivo heart rate variability (HRV) (SD of root mean square: 5.2 ± 1.4 ms vs. 1.4 ± 0.2 ms, p < 0.05) and Tpeak-Tend variability were higher in the running rabbits. Bradycardia disappeared in the exercised group in vitro. Dofetilide tended to increase the QTc interval in a greater extent, and significantly increased the number of arrhythmic beats in the trained animals in vitro. APD was longer in the exercised group at a low potassium level. Real-time quantitative PCR (RT-qPCR) showed significantly greater messenger RNA expression of fibrotic biomarkers in the exercised group. Increased repolarization variability and higher arrhythmia incidences, lengthened APD at a low potassium level, increased fibrotic biomarker gene expressions may indicate higher sensitivity of the rabbit “athlete’s heart” to life-threatening arrhythmias.
Physiological Mechanisms of Exercise and Its Effects on Postural Sway: Does Sport Make a Difference?
Authors: Zemková Erika
Published: February 2022

While the effect of a variety of exercises on postural balance control has been extensively studied, less attention has been paid to those requiring sport-specific skills. Therefore there is a need to analyze the literature and elucidate changes in postural balance control after exercises performed in conditions close to a particular sport. This scoping review aims (i) To map the literature that addresses postural sway aspects of acute responses to general and sport-specific exercises and their underlying physiological mechanisms, and (ii) To identify gaps in the existing literature and propose future research on this topic. The main literature search conducted on MEDLINE, Web of Science, Scopus, PubMed, and Cochrane Library databases was completed by SpringerLink, Elsevier, and Google Scholar. A total of 60 articles met the inclusion criteria. Findings identified that among a variety of studies evaluating the effects of exercise on postural balance control, only few of them were conducted under sport-specific conditions (i.e., while shooting in biathlon or pentathlon, and after simulated or match-induced protocols in combat and team sports). Therefore, more research is still needed to address this gap in the literature and aim research at investigation of postural sway response to sport-specific exercises. Further analysis of the literature showed that the type, intensity and duration of exercise play a key role in increased postural sway. Whole body and localized muscular fatigue of the trunk, neck and lower limbs is considered to be a main factor responsible for the magnitude of balance impairment in an initial phase of recovery and speed of its readjustment to a pre-exercise level. Other likely factors affecting postural stability are hyperventilation and deterioration of sensorimotor functions, though some contribution of muscle damage, dehydration, hyperthermia or dizziness cannot be excluded. A better understanding of the physiological mechanisms of balance impairment after exercises performed under simulated fatigue induced protocol, close to conditions specific to a particular sport, has implications for designing smart exercise programs tailored to individual needs to improve athlete performance with high demands on postural stability and/or decrease their risk of injuries.
A High-Quality, Chromosome-Level Genome Provides Insights Into Determinate Flowering Time and Color of Cotton Rose (Hibiscus mutabilis)
Authors: Yang Yuanzhao , Liu Xiaodan , Shi Xiaoqing , Ma Jiao , Zeng Xinmei , Zhu Zhangshun , Li Fangwen , Zhou Mengyan , Guo Xiaodan , Liu Xiaoli
Published: February 2022

Hibiscus mutabilis (cotton rose) is a deciduous shrub or small tree of the Malvaceae family. Here, we report a chromosome-scale assembly of the H. mutabilis genome based on a combination of single-molecule sequencing and Hi-C technology. We obtained an optimized assembly of 2.68 Gb with a scaffold N50 length of 54.7 Mb. An integrated strategy of homology-based, de novo, and transcriptome-based gene predictions identified 118,222 protein-coding genes. Repetitive DNA sequences made up 58.55% of the genome, and LTR retrotransposons were the most common repetitive sequence type, accounting for 53.15% of the genome. Through the use of Hi-C data, we constructed a chromosome-scale assembly in which Nanopore scaffolds were assembled into 46 pseudomolecule sequences. We identified important genes involved in anthocyanin biosynthesis and documented copy number variation in floral regulators. Phylogenetic analysis indicated that H. mutabilis was closely related to H. syriacus, from which it diverged approximately 15.3 million years ago. The availability of cotton rose genome data increases our understanding of the species’ genetic evolution and will support further biological research and breeding in cotton rose, as well as other Malvaceae species.
Low-Income Turkish Mothers’ Conceptions and Experiences of Family Life
Authors: Erdem Gizem , Adli-Isleyen Merve , Baltalarlı Nur , Kılıç Ezgi
Published: February 2022

The current qualitative study explores women’s conceptions of the normative family and their day-to-day family lives. To that aim, we conducted five focus group interviews in two low-income neighborhoods of Istanbul. The sample included 43 women (42 biological mothers and a grandmother) who had at least one child between ages 3 and 8 in their care. Participants were 35.64 years old on average (SD = 4.74) and were all married. Women had approximately two children (SD = 0.72) whose mean age was 7.92 years old (SD = 3.11). Each focus group was semi-structured, lasted for 1–1.5 h, and included 5–12 participants. Thematic analysis of the focus group interview data, moderator memos, and observer’s notes revealed five defining features of healthy family functioning: cohesion, healthy child, parenting, conflict, control, and family organization. Overall, women prioritized motherhood over their other social identities and idealized the happy family, which contradicted their actual lived experiences in the family system. We discuss how women’s depictions of all family processes revolved around cultural constructs of gender, socio-economic status, and independence/interdependence. The findings of this study shed light on future interventions for low-income women and their families in Turkey.
Neuromodulation to Treat Substance Use Disorders in People With Schizophrenia and Other Psychoses: A Systematic Review
Authors: Johnstone Samantha , Sorkhou Maryam , Al-Saghir Nada , Lowe Darby J. E. , Steele Vaughn R. , Pearlson Godfrey D. , Castle David J. , George Tony P.
Published: February 2022

Background

Substance use disorders (SUDs) are a common yet poorly studied comorbidity in individuals with psychotic disorders. The co-occurrence of the two complicates recovery and interferes with pharmacological and behavioral treatment response and adherence. Recently, researchers have been exploring both invasive and non-invasive neuromodulation techniques as potential treatment methods for SUDs. We review the evidence that neuromodulation may reduce substance craving and consumption in individuals with schizophrenia.

Methods

A comprehensive literature search of PubMed, MEDLINE, and PsycINFO databases was conducted (N = 1,432). Of these, we identified seven studies examining the effects of repetitive transcranial magnetic stimulation (rTMS) and two studies using transcranial direct current stimulation (tDCS) on drug consumption and craving in schizophrenia or schizoaffective disorders.

Results

Despite the limited number of studies in this area, the evidence suggests that rTMS to the dorsolateral prefrontal cortex (DLPFC) may reduce cannabis and tobacco use in patients with schizophrenia and schizoaffective disorder. Findings with tDCS, however, were inconclusive.

Discussion

Our systematic review suggests that rTMS applied to DLPFC is a safe and promising therapeutic technique for the management of comorbid schizophrenia and SUDs, with the majority of the evidence in tobacco use disorder. However, there was substantial heterogeneity in study methods, underscoring the need to optimize stimulation parameters (e.g., frequency, duration, and target regions). Larger clinical trials are needed to establish the efficacy of rTMS in reducing drug consumption and craving in psychotic patients, ideally in comparison to existing pharmacological and behavioral interventions.
A Cross-Sectional Study on the Cross-Talk of the COVID-19-Related Degree of Loneliness and the Etiological Factors Among the Elderly in Central China
Authors: Ju Jie , Qi Wen-bo , Zhang Jia , Cao Zhi-Jun , Tsai Chi-Lun , Liu Peng
Published: February 2022

Background

The outbreak of COVID-19 has undoubtedly influenced the normal lifestyle of people worldwide, including the Chinese population. This study attempted to do supplementary research to the current situation of loneliness as well as the related risk factors among the elderly in the province in central Chinese during the COVID-19.

Methods

We conducted a cross-sectional study in one of the central Chinese provinces (Henan Province) from December 2020 to March 2021 using a multistage sampling method, and 568 elderly people without cognitive impairment were interviewed. The UCLA Loneliness Scale, Pittsburgh Sleep Quality Index (PSQI), Physical Activity Rating Scale (PARS-3), and Quality of Life Questionnaire SF-36 were adopted to collect information. We used univariate and multivariate logistic regressions to analyze the factors resulting in severe loneliness among the elderly with seldom or regular participation in physical exercises.

Results

During the epidemic in central China, the elderly suffering from loneliness syndrome accounted for 34.2%, of which 15.5% were severely lonely. Risk factors for severe loneliness were quality of life (OR: 7.129), sleep quality (OR: 3.778), seldom exercise (OR: 4.170), poor economic status (OR: 1.769), and negative attitude toward the prospects for the epidemic control (OR: 4.033). By grouping the participants in terms of physical activity, we found that the quality of life (OR:5.778) was a significant risk factor than sleep quality (OR:2.939) in the seldom exercise group, while the only risk factor in the regular exercise group was the quality of life (OR: 5.021).

Conclusion

There was an increase in the degree of loneliness among the elderly during the epidemic, and physical activity played an active role in relieving the severe loneliness of the elderly. Therefore, for the sake of the elderly, regular participation in physical exercises should be encouraged during the duration of the epidemic.
Family Function Impacts Relapse Tendency in Substance Use Disorder: Mediated Through Self-Esteem and Resilience
Authors: Xia Yuwei , Gong Yu , Wang Hanbin , Li Shen , Mao Fuqiang
Published: February 2022

Background

Substance abuse has been a public health concern, and even after detoxification treatment, the relapse rate is still high. Family function is closely related to substance dependence. However, studies on psychological mechanisms between them are rare.

Objectives

We aimed to explore the mediating role of self-esteem and resilience in the pathway that family function impacts the relapse tendency among patients with substance use disorder (SUD).

Methods

A total of 282 SUD patients were recruited, and standard questionnaires were administered for each patient. The relapse tendency, family function, self-esteem and resilience were assessed by the family care index questionnaire, the Connor-Davidson resilience scale, the Rosenberg self-esteem scale and the relapse tendency questionnaire. Bootstrap method was conducted for mediation analysis to test the effects of how family function affects relapse tendency mediated through self-esteem and resilience.

Results

The average score of relapse tendency of the patients was 28.47 (SD = 11.89). Intermediary analysis found that self-esteem played an intermediary role in the relationship between family function and relapse tendency. Resilience plays an intermediary role in the relationship between family function and relapse tendency. Further, the path analysis showed that family function not only had a direct association with relapse tendency, but also indirectly related to relapse tendency through self-esteem and resilience.

Conclusions

Self-esteem and resilience are the key factors in the relationship between family function and relapse tendency of SUD patients.
The Knowledge and Attitude of Parents About the Dental Treatment of Their Children During the New Type of Coronavirus Outbreak in Northern Cyprus
Authors: Ekinci Ayse , Tosun Ozgur , Islam Aylin
Published: February 2022

Background

The aim of this study was to assess the knowledge and attitude of parents living in Northern Cyprus about the oral care and dental treatments of their children during the outbreak of the new type of Coronavirus (COVID-19).

Methods

An online self-administered questionnaire was conducted consisting of 33 questions. A total of 256 parents participated in this study. The questionnaire was divided into two parts. The first part consists of demographic information and the second part consists of the awareness and knowledge of parents about dental treatments during COVID-19. SPSS software was used for statistical data analysis.

Results

In total, 81.9% of the mothers and 59.6% of the fathers stated that their children could be infected with COVID-19 during dental treatments and were apprehensive about their children undergoing such treatment because of the outbreak. Participants were asked about their knowledge regarding the transmission of COVID-19 through air droplets during dental treatments and the findings revealed that mothers (89.5%) were more aware of this than fathers (77.2%). The majority of the participants (77%) stated that their children could become infected with COVID-19 during dental treatments and were apprehensive about their children undergoing such treatment because of the outbreak; however, 65.1% did not take any extra precautions regarding their children's oral health and care.

Conclusion

The majority of the parents in Northern Cyprus have good knowledge about dental clinics being one of the high-risk areas where COVID-19 can be transmitted by aerosols from infected people. However, further steps need to be taken to strengthen parents' motivations for home oral care and more studies are needed to assess the continuing impact of the COVID-19 pandemic on parents' attitudes toward and knowledge about dental procedures.
Finding Hamiltonian and Longest (s,t)-Paths of C-Shaped Supergrid Graphs in Linear Time
Authors: Keshavarz-Kohjerdi Fatemeh , Hung Ruo-Wei
Published: February 2022

A graph is called Hamiltonian connected if it contains a Hamiltonian path between any two distinct vertices. In the past, we proved the Hamiltonian path and cycle problems for general supergrid graphs to be NP-complete. However, they are still open for solid supergrid graphs. In this paper, first we will verify the Hamiltonian cycle property of C-shaped supergrid graphs, which are a special case of solid supergrid graphs. Next, we show that C-shaped supergrid graphs are Hamiltonian connected except in a few conditions. For these excluding conditions of Hamiltonian connectivity, we compute their longest paths. Then, we design a linear-time algorithm to solve the longest path problem in these graphs. The Hamiltonian connectivity of C-shaped supergrid graphs can be applied to compute the optimal stitching trace of computer embroidery machines, and construct the minimum printing trace of 3D printers with a C-like component being printed.
Occupational Safety Climate in the Swedish Equine Sector
Authors: Lindahl Cecilia , Bergman Bruhn Åsa , Andersson Ing-Marie
Published: February 2022

The Swedish equine sector is considered a high-risk work environment, with relatively high injury rates and high severity of injuries. General safety research has identified a correlation between the safety performance and safety culture, but little is known about the intricacies of the safety culture in the Swedish equine sector, especially concerning managers’ and employees’ perceptions of their work environment. The safety climate assessment is recognised as an effective tool for identifying potential problems in the workplace, thus enhancing safety behaviour and decreasing the frequency and severity of injuries. The aim was to evaluate the safety climate at riding schools and trotting stables through the Nordic Safety Climate Questionnaire (NOSACQ-50) diagnostic tool, and to get a better understanding of the workers’ perceptions regarding safety and safety management at their workplace through complementary interviews. The results showed that the safety climate was generally positive and that employees were aware of the risks relating to their work. Riding schools commonly had routines in place for risk assessment and work environment management, but such routines were often lacking at trotting stables, indicating inadequate prioritisation of safety by the management. The main area that should be targeted to improve safety in the sector is employees’ prioritisation and non-acceptance of risks. Proactive instead of reactive safety management should be promoted, where safety is an integral part of daily work and all employees are encouraged to identify factors contributing to occupational injuries and develop strategies for injury prevention.
Associations between Exercise-Induced Pulmonary Hemorrhage (EIPH) and Fitness Parameters Measured by Incremental Treadmill Test in Standardbred Racehorses
Authors: Lo Feudo Chiara Maria , Stucchi Luca , Stancari Giovanni , Alberti Elena , Conturba Bianca , Zucca Enrica , Ferrucci Francesco
Published: February 2022

Exercise-induced pulmonary hemorrhage (EIPH) is a condition affecting up to 95% of racehorses, diagnosed by detecting blood in the trachea after exercise and/or the presence of hemosiderophages in the bronchoalveolar lavage fluid (BALf). Although EIPH is commonly associated with poor performance, scientific evidence is scarce. The athletic capacity of racehorses can be quantified through some parameters obtained during an incremental treadmill test; in particular, the speed at a heart rate of 200 bpm (V200), and the speed (VLa4) and the heart rate (HRLa4) at which the blood lactate concentration reaches 4 mmol/L are considered good fitness indicators. The present retrospective study aims to evaluate whether EIPH could influence fitness parameters in poorly performing Standardbreds. For this purpose, data from 81 patients regarding their V200, VLa4, HRLa4, peak lactate, maximum speed, minimum pH, and maximum hematocrit were reviewed; EIPH scores were assigned based on tracheobronchoscopy and BALf cytology. The association between the fitness parameters and EIPH was evaluated through Spearman’s correlation analysis. No relationship between EIPH and V200, VLa4, and HRLa4 was observed. Interestingly, EIPH-positive horses showed higher hematocrit values (p = 0.0072, r = 0.47), suggesting the possible influence of the hemoconcentration on the increase of pulmonary capillary pressure as a part of the pathogenesis of EIPH.
Heterologous Challenge with PRRSV-1 MLV in Pregnant Vaccinated Gilts: Potential Risk on Health and Immunity of Piglets
Authors: Papakonstantinou Georgios , Meletis Eleftherios , Christodoulopoulos Georgios , Tzika Eleni D. , Kostoulas Polychronis , Papatsiros Vasileios G.
Published: February 2022

The objective of the present study was to evaluate the potential risks of the four commercial PRRS-1 MLV vaccines in pregnant vaccinated gilts at the last stage of gestation under field conditions. The study was conducted at four pig farms, including 25 gilts from each farm (25 × 4 = 100 gilts), which were equally allocated to five different study groups. A PRRS-1 MLV vaccination was applied on the 100th day of their pregnancy with the different commercial vaccines that are available in the Greek market. The results indicated virus congenital infection and viremia in piglets (20/200 = 10% PRRSV infected piglets), and detection of PRRSV-specific antibodies (181/200 = 90.5% piglets found with PRRSV antibodies). The subsequent phylogenetic analyses revealed high percentages of similarity between the PRRSV-1 strain detected in infected litters and the PRRSV-1 vaccine strain to which the study gilts had been previously exposed to. Health status analyses of trial piglets resulted in differences between litters from vaccinated sows and litters from non-vaccinated sows at 110th day of gestation as regards the number of weak-born piglets, mummies, and piglets with splay-leg and/or respiratory symptoms. The current study’s results indicate several potential dangers of the PRRS MLV vaccination in late gestation.
Antioxidant Status, Blood Constituents and Immune Response of Broiler Chickens Fed Two Types of Diets with or without Different Concentrations of Active Yeast
Authors: Attia Youssef A. , Al-Khalaifah Hanan , Abd El-Hamid Hatem S. , Al-Harthi Mohammed A. , Alyileili Salem R. , El-Shafey Ali A.
Published: February 2022

Probiotics, such as active yeasts, are widely used to enhance poultry production and reduce feeding costs. This study aimed to investigate the antioxidant and immune responses of broilers to different concentrations of active Saccharomyces cerevisiae (SC) when supplemented to two types of diets. A total of 216 1-day-old Arbor Acres unsexed chicks were used in a factorial design, involving two feeds (regular- versus low-density diet) and three concentrations of SC (0%, 0.02% and 0.04%). The results revealed that the low-density diet reduced the body weight and production index of broilers. The addition of SC improved the production index more than the control diet. Total antioxidant capacity (TAC), alanine aminotransferase (ALT), aspartate aminotransferase (AST) and eosinophils were significantly higher in response to the regular-density diet than the low-density diet; however, phagocytic activity (PA), lymphocyte and lysozyme activity (LYS) were lower. Saccharomyces cerevisiae reduced ALT, AST, malondialdehyde (MAD) and TAC more than the standard set, but improved packed cell volume (PCV), hemoglobin (Hgb), red blood cells (RBCs), lymphocytes, monocytes, heterophils, phagocytic index (PI) and the immune response to Newcastle disease virus (NDV) and avian influenza (AI). In conclusion, supplementation of a regular- or low-density diet with SC at a concentration of 0.02% or 0.04% improved the antioxidant parameters, immune status and production index of broilers against stress and infectious agents.
AidIR: An Interactive Dialog System to Aid Disease Information Retrieval
Authors: Wang Da-Jinn , Chen Tsong-Yi , Su Chia-Yi
Published: February 2022

This paper proposes an interactive dialog system, called AidIR, to aid information retrieval. AidIR allows users to retrieve information on diseases resulting from coronaviruses and diseases transmitted by vector mosquitoes with natural language interaction and Line chat media. In a subjective evaluation, we asked 20 users to rate the intuitiveness, usability, and user experience of AidIR with a range between −2 and 2. Moreover, we also asked these users to answer yes–no questions to evaluate AidIR and provide feedback. The average scores of intuitiveness, usability, and user experience are 0.8, 0.8, and 1.05, respectively. The yes–no questions demonstrated that AidIR is better than systems using the graphical user interface in mobile phones and single-turn dialog systems. According to user feedback, AidIR is more convenient for information retrieval. Moreover, we designed a new loss function to jointly train a BERT model for domain classification and sequence label tasks. The accuracy of both tasks is 92%. Finally, we trained the dialog policy network with supervised learning tasks and deployed the reinforcement learning algorithm to allow AidIR to continue learning the dialog policy.