bluuebunny/crossref_metadata_2025 huggingface dataset:
Fabrication of Metallic Superhydrophobic Surfaces with Tunable Condensate Self-Removal Capability and Excellent Anti-Frosting Performance
Authors: He Jian-Guo , Zhao Guan-Lei , Dai Shou-Jun , Li Ming , Zou Gui-Sheng , Wang Jian-Jun , Liu Yang , Yu Jia-Qi , Xu Liang-Fei , Li Jian-Qiu , Fan Lian-Wen , Huang Min
Published: October 2022

Laser fabrication of metallic superhydrophobic surfaces (SHSs) for anti-frosting has recently attracted considerable attention. Effective anti-frosting SHSs require the efficient removal of condensed microdroplets through self-propelled droplet jumping, which is strongly influenced by the surface morphology. However, detailed analyses of the condensate self-removal capability of laser-structured surfaces are limited, and guidelines for laser processing parameter control for fabricating rationally structured SHSs for anti-frosting have not yet been established. Herein, a series of nanostructured copper-zinc alloy SHSs are facilely constructed through ultrafast laser processing. The surface morphology can be properly tuned by adjusting the laser processing parameters. The relationship between the surface morphologies and condensate self-removal capability is investigated, and a guideline for laser processing parameterization for fabricating optimal anti-frosting SHSs is established. After 120 min of the frosting test, the optimized surface exhibits less than 70% frost coverage because the remarkably enhanced condensate self-removal capability reduces the water accumulation amount and frost propagation speed (<1 μm/s). Additionally, the material adaptability of the proposed technique is validated by extending this methodology to other metals and metal alloys. This study provides valuable and instructive insights into the design and optimization of metallic anti-frosting SHSs by ultrafast laser processing.
Self-Healable Lithium-Ion Batteries: A Review
Authors: Cheng Ye , Wang Chengrui , Kang Feiyu , He Yan-Bing
Published: October 2022

The inner constituents of lithium-ion batteries (LIBs) are easy to deform during charging and discharging processes, and the accumulation of these deformations would result in physical fractures, poor safety performances, and short lifespan of LIBs. Recent studies indicate that the introduction of self-healing (SH) materials into electrodes or electrolytes can bring about great enhancements in their mechanical strength, thus optimizing the cycle stability of the batteries. Due to the self-healing property of these special functional materials, the fractures/cracks generated during repeated cycles could be spontaneously cured. This review systematically summarizes the mechanisms of self-healing strategies and introduces the applications of SH materials in LIBs, especially from the aspects of electrodes and electrolytes. Finally, the challenges and the opportunities of the future research as well as the potential of applications are presented to promote the research of this field.
Effect of Ag-Decorated BiVO4 on Photoelectrochemical Water Splitting: An X-ray Absorption Spectroscopic Investigation
Authors: Nga Ta Thi Thuy , Huang Yu-Cheng , Chen Jeng-Lung , Chen Chi-Liang , Lin Bi-Hsuan , Yeh Ping-Hung , Du Chao-Hung , Chiou Jau-Wern , Pong Way-Faung , Arul K. Thanigai , Dong Chung-Li , Chou Wu-Ching
Published: October 2022

Bismuth vanadate (BiVO4) has attracted substantial attention on account of its usefulness in producing hydrogen by photoelectrochemical (PEC) water splitting. The exploitation of BiVO4 for this purpose is yet limited by severe charge recombination in the bulk of BiVO4, which is caused by the short diffusion length of the photoexcited charge carriers and inefficient charge separation. Enormous effort has been made to improve the photocurrent density and solar-to-hydrogen conversion efficiency of BiVO4. This study demonstrates that modulating the composition of the electrode and the electronic configuration of BiVO4 by decoration with silver nanoparticles (Ag NPs) is effective in not only enhancing the charge carrier concentration but also suppressing charge recombination in the solar water splitting process. Decoration with a small number of Ag NPs significantly enhances the photocurrent density of BiVO4 to an extent that increases with the concentration of the Ag NPs. At 0.5% Ag NPs, the photocurrent density approaches 4.1 mA cm−2 at 1.23 V versus a reversible hydrogen electrode (RHE) under solar simulated light illumination; this value is much higher than the 2.3 mA cm−2 of pure BiVO4 under the same conditions. X-ray absorption spectroscopy (XAS) is utilized to investigate the electronic structure of pure BiVO4 and its modification by decoration with Ag NPs. Analytical results indicate that increased distortion of the VO4 tetrahedra alters the V 3d–O 2p hybridized states. Additionally, as the Ag concentration increases, the oxygen vacancy defects that act as recombination centers in BiVO4 are reduced. In situ XAS, which is conducted under dark and solar illumination conditions, reveals that the significantly enhanced PEC performance is attributable to the synergy of modulated atomic/electronic structures and the localized surface plasmon resonance effect of the Ag nanoparticles.
Quasiparticle Self-Consistent GW Study of Simple Metals
Authors: Friedrich Christoph , Blügel Stefan , Nabok Dmitrii
Published: October 2022

The GW method is a standard method to calculate the electronic band structure from first principles. It has been applied to a large variety of semiconductors and insulators but less often to metallic systems, in particular, with respect to a self-consistent employment of the method. In this work, we take a look at all-electron quasiparticle self-consistent GW (QSGW) calculations for simple metals (alkali and alkaline earth metals) based on the full-potential linearized augmented-plane-wave approach and compare the results to single-shot (i.e., non-selfconsistent) G0W0 calculations, density-functional theory (DFT) calculations in the local-density approximation, and experimental measurements. We show that, while DFT overestimates the bandwidth of most of the materials, the GW quasiparticle renormalization corrects the bandwidths in the right direction, but a full self-consistent calculation is needed to consistently achieve good agreement with photoemission data. The results mainly confirm the common belief that simple metals can be regarded as nearly free electron gases with weak electronic correlation. The finding is particularly important in light of a recent debate in which this seemingly established view has been contested.
Coating of Flexible PDMS Substrates through Matrix-Assisted Pulsed Laser Evaporation (MAPLE) with a New-Concept Biocompatible Graphenic Material
Authors: Alfe Michela , Minopoli Giuseppina , Tartaglia Massimiliano , Gargiulo Valentina , Caruso Ugo , Pepe Giovanni Piero , Ausanio Giovanni
Published: October 2022

In this study, matrix-assisted pulsed laser evaporation (MAPLE) was used to deposit graphene-like materials (GL), a new class of biocompatible graphene-related materials (GRMs) obtained from a controlled top-down demolition of a carbon black, on silicone slices to test their potential use as functional coating on invasive medical devices as indwelling urinary catheters. Results indicate that the relevant chemical-physical features of the deposit (controlled by FTIR and AFM) were maintained after MAPLE deposition. After deposition, GL films underwent a biological survey toward target cellular lines (murine fibroblast NIH3T3, human keratinocytes HaCAT and the human cervical adenocarcinoma epithelial-like HeLa). Results indicate that the GL films did not lead to any perturbations in the different biological parameters evaluated. The presented results and the possibility to further functionalize the GL or combine them with other functional materials in a hybrid fashion to assure a tighter adhesion onto the substrate for use in harsh conditions open the door to practical applications of these new-concept medical devices (drug delivery, next generation flexible devices, multifunctional coatings) paving the way to the prevention of nosocomial infections driven by catheterization through antibiotics-free approaches.
Improved Photocatalytic Activity via n-Type ZnO/p-Type NiO Heterojunctions
Authors: Ma Ligang , Ai Xiaoqian , Chen Yujie , Liu Pengpeng , Lin Chao , Lu Kehong , Jiang Wenjun , Wu Jiaen , Song Xiang
Published: October 2022

The design and construct pn heterojunction to reduce the recombination rate of photogenerated electron-hole pairs can effectively improve photocatalytic activity. In this study, ZnO/NiO heterojunctions were fabricated by annealing a Zn/Ni metal organic framework precursor synthesized via coprecipitation. The effects of the precursor annealing temperature on the microstructure, morphology, and optical properties of the ZnO/NiO nanocomposites were investigated using X-ray diffraction, scanning electron microscopy, transmission electron microscopy, X-ray photoelectron spectroscopy, and UV-vis absorption spectroscopy. The results showed that the nanocomposite was composed of hexagonal wurtzite ZnO and cubic NiO, with the former being the dominant phase. Large ZnO nanoparticles were attached to small NiO nanoparticles, and a pn heterojunction interface was formed. The photodegradation performance of the nanomaterials was evaluated by monitoring the degradation of RhB under irradiation by ultraviolet light. The ZnO/NiO nanocomposites exhibited excellent photocatalytic activity when the annealing temperature was 550 °C. The photodegradation mechanism was also analyzed in detail, revealing that the heterojunction between the n-type ZnO and the p-type NiO played an important role in impeding the recombination of photogenerated electron-hole pairs and improving the photocatalytic efficiency.
Processing Effects on the Martensitic Transformation and Related Properties in the Ni55Fe18Nd2Ga25 Ferromagnetic Shape Memory Alloy
Authors: Sofronie Mihaela , Popescu Bogdan , Enculescu Monica , Tolea Mugurel , Tolea Felicia
Published: October 2022

The influence of processing on the martensitic transformation and related magnetic properties of the Ni55Fe18Nd2Ga25 ferromagnetic shape memory alloy, as bulk and ribbons prepared by the melt spinning method and subjected to different thermal treatments, is investigated. Structural, calorimetric, and magnetic characterizations are performed. Thermal treatment at 1173 K induces a decrease in both the Curie and the martensitic transformation temperatures, while a treatment at 673 K produces the structural ordering of the ribbons, hence an increase in TC. A maximum value of the magnetic entropy variation of −5.41 J/kgK was recorded at 310 K for the as quenched ribbons. The evaluation of the magnetoresistive effect shows a remarkable value of −13.5% at 275 K on the bulk sample, which is much higher than in the ribbons.
The Impact of a Family-Based Assessment and Intervention Healthy Lifestyle Programme on Health Knowledge and Beliefs of Children with Obesity and Their Families
Authors: Te’o Dayna T. , Wild Cervantée E. K. , Willing Esther J. , Wynter Lisa E. , O’Sullivan Niamh A. , Hofman Paul L. , Maessen Sarah E. , Derraik José G. B. , Anderson Yvonne C.
Published: October 2022

Objective: To determine the impact of a family-based assessment-and-intervention healthy lifestyle programme on health knowledge and beliefs of children and families affected by obesity. Second, to compare the health knowledge of the programme cohort to those of a national cohort in Aotearoa/New Zealand (NZ). Design: This mixed-methods study collected health knowledge and health belief data in a questionnaire at baseline and 12-, 24-, and 60-month follow-up assessments. Health knowledge over time was compared with baseline knowledge and with data from a nationally representative survey. A data-driven subsumption approach was used to analyse open-text responses to health belief questions across the study period. Setting: Taranaki region, a mixed urban–rural setting in NZ. Participants: Participants (caregiver/child dyads) from the Whānau Pakari randomised trial. Results: A greater proportion of the cohort correctly categorised foods and drinks as healthy or unhealthy at 12 months compared to baseline for most questionnaire items. Retention of this health knowledge was evident at 24- and 60-month follow-ups. More than twice as many participants correctly reported physical activity recommendations at follow-up compared to baseline (p < 0.001). Health knowledge of participants was similar to the national survey cohort at baseline, but surpassed it at 12 and 24 months. Participant beliefs around healthy lifestyles related to physical functioning, mental and emotional wellbeing, and enhancement of appearance, and gained greater depth and detail over time. Conclusions: This study demonstrates the important role that community-level healthy lifestyle programmes can have in knowledge-sharing and health promotion.
Statin-Induced Geranylgeranyl Pyrophosphate Depletion Promotes Ferroptosis-Related Senescence in Adipose Tissue
Authors: Shu Xin , Wu Jiaqi , Zhang Tao , Ma Xiaoyu , Du Zuoqin , Xu Jin , You Jingcan , Wang Liqun , Chen Ni , Luo Mao , Wu Jianbo
Published: October 2022

Statin treatment is accepted to prevent adverse cardiovascular events. However, atorvastatin, an HMG-CoA reductase inhibitor, has been reported to exhibit distinct effects on senescent phenotypes. Whether atorvastatin can induce adipose tissue senescence and the mechanisms involved are unknown. The effects of atorvastatin-induced senescence were examined in mouse adipose tissue explants. Here, we showed that statin initiated higher levels of mRNA related to cellular senescence markers and senescence-associated secretory phenotype (SASP), as well as increased accumulation of the senescence-associated β-galactosidase (SA-β-gal) stain in adipose tissues. Furthermore, we found that the levels of reactive oxygen species (ROS), malondialdehyde (MDA), and Fe2+ were elevated in adipose tissues treated with atorvastatin, accompanied by a decrease in the expression of glutathione (GSH), and glutathione peroxidase 4 (GPX4), indicating an iron-dependent ferroptosis. Atorvastatin-induced was prevented by a selective ferroptosis inhibitor (Fer-1). Moreover, supplementation with geranylgeranyl pyrophosphate (GGPP), a metabolic intermediate, reversed atorvastatin-induced senescence, SASP, and lipid peroxidation in adipose tissue explants. Atorvastatin depleted GGPP production, but not Fer-1. Atorvastatin was able to induce ferroptosis in adipose tissue, which was due to increased ROS and an increase in cellular senescence. Moreover, this effect could be reversed by the supplement of GGPP. Taken together, our results suggest that the induction of ferroptosis contributed to statin-induced cell senescence in adipose tissue.
Anti-Aging and Neuroprotective Properties of Grifola frondosa and Hericium erinaceus Extracts
Authors: Tripodi Farida , Falletta Ermelinda , Leri Manuela , Angeloni Cristina , Beghelli Daniela , Giusti Laura , Milanesi Riccardo , Sampaio-Marques Belém , Ludovico Paula , Goppa Lorenzo , Rossi Paola , Savino Elena , Bucciantini Monica , Coccetti Paola
Published: October 2022

Nutrition has relevant consequences for human health and increasing pieces of evidence indicate that medicinal mushrooms have several beneficial effects. One of the main issues in Western countries is represented by the challenges of aging and age-related diseases, such as neurodegenerative disorders. Among these, Parkinson’s disease (PD) affects 10 million people worldwide and is associated with α-synuclein misfolding, also found in other pathologies collectively called synucleinopathies. Here, we show that aqueous extracts of two edible mushrooms, Grifola frondosa and Hericium erinaceus, represent a valuable source of β-glucans and exert anti-aging effects in yeast. Their beneficial effects are mediated through the inhibition of the Ras/PKA pathway, with increased expression of heat shock proteins, along with a consistent increase of both mean and maximal lifespans. These fungal extracts also reduce the toxicity of α-synuclein heterologously expressed in yeast cells, resulting in reduced ROS levels, lower α-synuclein membrane localization, and protein aggregation. The neuroprotective activity of G. frondosa extract was also confirmed in a PD model of Drosophila melanogaster. Taken together, our data suggest the use of G. frondosa and H. erinaceus as functional food to prevent aging and age-related disorders, further supporting the neuro-healthy properties of these medicinal mushroom extracts.
Determinants of Complementary Feeding Indicators: A Secondary Analysis of Thailand Multiple Indicators Cluster Survey 2019
Authors: Supthanasup Abhirat , Cetthakrikul Nisachol , Kelly Matthew , Sarma Haribondhu , Banwell Cathy
Published: October 2022

Child complementary feeding (CF) practices meet dietary recommendations more often among educated, high-income groups. Much of the evidence for this association addresses inadequate CF for addressing child undernutrition. However, in many countries, including Thailand, child malnutrition assessments must now address under- and over-nutrition. More comprehensive data is needed to understand this complex situation. This study uses data from the Thailand Multiple Indicators Survey 2019, to identify the determinants of CF practices among 6–23-month children (n = 4125) using the newly developed WHO indicators. Logistic regression analysis was used to measure associations between sociodemographic factors and CF practices. In a fully adjusted model, child age, primary caregivers’ education, and household incomes were statistically associated with (in)appropriate CF practices. Older children aged 9–23 months, not only have better minimum dietary diversity (MDD), minimum acceptable diet (MAD), and egg and/or flesh food consumption (EFF), but also tend to consume more unhealthy foods. The proportion of inappropriate CF practices was higher among children living with caregivers other than their mothers. While maternal education and household income were positively associated with MDD and MAD, children of mothers from middle-class households consumed more sweetened beverages. Therefore, nutrition programs addressing different feeding problems should be developed specifically for different primary caregiver and demographic groups.
Ketone Ester Supplementation Improves Some Aspects of Cognitive Function during a Simulated Soccer Match after Induced Mental Fatigue
Authors: Quinones Manuel D. , Lemon Peter W. R.
Published: October 2022

Ketone supplementation has been proposed to enhance cognition during exercise. To assess whether any benefits are due to reduced cognitive fatigue during the latter portions of typical sport game action, we induced cognitive fatigue, provided a ketone monoester supplement (KME) vs. a non-caloric placebo (PLAC), and assessed cognitive performance during a simulated soccer match (SSM). In a double-blind, balanced, crossover design, nine recreationally active men (174.3 ± 4.2 cm, 76.6 ± 7.4 kg, 30 ± 3 y, 14.2 ± 5.5 % body fat, V˙O2 max = 55 ± 5 mL·kg BM−1·min−1; mean ± SD) completed a 45-min SSM (3 blocks of intermittent, variable intensity exercise) consuming either KME (25 g) or PLAC, after a 40-min mental fatiguing task. Cognitive function (Stroop and Choice Reaction Task [CRT]) and blood metabolites were measured throughout the match. KME reduced concentrations of both blood glucose (block 2: 4.6 vs. 5.2 mM, p = 0.02; block 3: 4.7 vs. 5.3 mM, p = 0.01) and blood lactate (block 1: 4.7 vs. 5.4 mM, p = 0.05; block 2: 4.9 vs. 5.9 mM, p = 0.01) during the SSM vs. PLAC, perhaps indicating a CHO sparing effect. Both treatments resulted in impaired CRT performance during the SSM relative to baseline, but KME displayed a reduced (p < 0.05) performance decrease compared to PLAC (1.3 vs. 3.4% reduction in correct answers, p = 0.02). No other differences in cognitive function were seen. These data suggest that KME supplementation attenuated decrements in CRT during repeated, high intensity, intermittent exercise. More study is warranted to assess fully the potential cognitive/physical benefits of KME for athletes.
Development of Capsaicin-Containing Analgesic Silicone-Based Transdermal Patches
Authors: László Szabolcs , Bátai István Z. , Berkó Szilvia , Csányi Erzsébet , Dombi Ágnes , Pozsgai Gábor , Bölcskei Kata , Botz Lajos , Wagner Ödön , Pintér Erika
Published: October 2022

Transdermal therapeutic systems (TTSs) enable convenient dosing in drug therapy. Modified silicone-polymer-based patches are well-controlled and cost-effective matrix diffusion systems. In the present study, we investigated the substance release properties, skin penetration, and analgesic effect of this type of TTS loaded with low-dose capsaicin. Release properties were measured in Franz diffusion cell and continuous flow-through cell approaches. Capsaicin was detected with HPLC-UV and UV spectrophotometry. Raman spectroscopy was conducted on human skin samples exposed to the TTS. A surgical incision or carrageenan injection was performed on one hind paw of male Wistar rats. TTSs were applied to the epilated dorsal skin. Patches were kept on the animals for 6 h. The thermal hyperalgesia and mechanical pain threshold of the hind paws were detected. Patches exhibited controlled, zero-order kinetic capsaicin release. According to the Raman mapping, capsaicin penetrated into the epidermis and dermis of human skin, where the target receptors are expressed. The thermal pain threshold drop of the operated rat paws was reversed by capsaicin treatment compared to that of animals treated with control patches. It was concluded that our modified silicone-polymer-based capsaicin-containing TTS is suitable for the relief of traumatic and inflammatory pain.
Autologous Genetically Enriched Leucoconcentrate in the Preventive and Acute Phases of Stroke Treatment in a Mini-Pig Model
Authors: Safiullov Zufar , Izmailov Andrei , Sokolov Mikhail , Markosyan Vage , Kundakchan Grayr , Garifulin Ravil , Shmarov Maksim , Naroditsky Boris , Logunov Denis , Islamov Rustem
Published: October 2022

The natural limitations of regeneration in the CNS are major problems for the treatment of neurological disorders, including ischaemic brain strokes. Among the approaches being actively developed to inhibit post-ischaemic negative consequences is the delivery of therapeutic genes encoding neuroprotective molecules to the brain. Unfortunately, there are currently no proven and available medicines that contain recombinant human genes for the treatment of ischaemic cerebral stroke. Of particular interest is the development of treatments for patients at risk of ischaemic stroke. In the present study, we propose a proof of concept for the use of an autologous, genetically enriched leucoconcentrate temporally secreting recombinant vascular endothelial growth factor (VEGF), glial-cell-line-derived neurotrophic factor (GDNF) and the neural cell adhesion molecule (NCAM) for the treatment of stroke. In a mini-pig ischaemic stroke model, genetically enriched leucoconcentrate was infused 4 h after surgery (gene therapy in acute phase) or 2 days before stroke modelling (preventive gene therapy). On day 21, after the stroke modelling, the post-ischaemic brain recovery was examined by morphologic and immunofluorescence analysis. The benefits of treating a stroke with genetically enriched leucoconcentrate both for preventive purposes and in the acute phase were confirmed by an improved performance in behavioural tests, higher preservation of brain tissue and positive post-ischaemic brain remodelling in the peri-infarct area. These results suggest that the employment of autologous leucocytes enabling the temporary production of the recombinant therapeutic molecules to correct the pathological process in the CNS may be one of the breakthrough approaches in gene therapy.
Novel Stenotrophomonas maltophilia Bacteriophage as Potential Therapeutic Agent
Authors: Fanaei Pirlar Rima , Wagemans Jeroen , Kunisch Fabian , Lavigne Rob , Trampuz Andrej , Gonzalez Moreno Mercedes
Published: October 2022

A novel bacteriophage CUB19 specific to the bacterial species Stenotrophomonas maltophilia was isolated from hospital sewage and characterized as a new species belonging to a proposed new phage genus ‘Cubvirus’ (Caudoviricetes). Its genome contains a total of 48,301 bp and 79 predicted genes, among which some have been associated with packaging and lysis-associated proteins, structural proteins, or DNA- and metabolism-associated proteins. No lysogeny-associated proteins or known virulence proteins were identified on the phage genome. CUB19 showed stability over a wide range of temperatures (−20 °C–60 °C) and pH values (pH 3–pH 13). Despite its narrow host range, this phage has potent observed antimicrobial and antibiofilm activity. A time-killing curve assay showed significant biofilm reduction after 24 h exposure to CUP19. Isothermal microcalorimetry assays investigating phage-antibiotic combinations revealed the effectiveness of CUB19 during co-administration with increasing antibiotic doses, regardless of the administration approach (simultaneous or staggered). These are encouraging indications for its application as a targeted therapeutic agent against resilient biofilm-associated Stenotrophomonas infections.
Aptamer-Functionalized Nanoparticles Mediate PD-L1 siRNA Delivery for Effective Gene Silencing in Triple-Negative Breast Cancer Cells
Authors: Camorani Simona , Tortorella Silvia , Agnello Lisa , Spanu Chiara , d’Argenio Annachiara , Nilo Roberto , Zannetti Antonella , Locatelli Erica , Fedele Monica , Comes Franchini Mauro , Cerchia Laura
Published: October 2022

Small interfering RNA (siRNA) therapies require effective delivery vehicles capable of carrying the siRNA cargo into target cells. To achieve tumor-targeting, a drug delivery system would have to incorporate ligands that specifically bind to receptors expressed on cancer cells to function as portals via receptor-mediated endocytosis. Cell-targeting and internalizing aptamers are the most suitable ligands for functionalization of drug-loaded nanocarriers. Here, we designed a novel aptamer-based platform for the active delivery of siRNA targeting programmed cell death-ligand 1 (PD-L1) to triple-negative breast cancer (TNBC) cells. The generated nanovectors consist of PLGA-based polymeric nanoparticles, which were loaded with PD-L1 siRNA and conjugated on their surface with a new RNA aptamer, specific for TNBC and resistant to nucleases. In vitro results demonstrated that these aptamer-conjugated nanoparticles promote siRNA uptake specifically into TNBC MDA-MB-231 and BT-549 target cells, along with its endosomal release, without recognizing non-TNBC BT-474 breast cancer cells. Their efficiency resulted in an almost complete suppression of PD-L1 expression as early as 90 min of cell treatment. This research provides a rational strategy for optimizing siRNA delivery systems for TNBC treatments.
Overexpression of Nicotianamine Synthase (AtNAS1) Increases Iron Accumulation in the Tuber of Potato
Authors: Zha Manrong , Li Xin , Li Rui , Huang Jing , Fan Jinping , Zhang Jing , Wang Yan , Zhang Cankui
Published: October 2022

Iron (Fe) deficiency is a global health problem, especially in underdeveloped countries. Biofortification with genetic engineering methods has been used to improve Fe nutrition in a number of crops. Various steps, e.g., uptake, distribution, and storage, involved in Fe homeostasis have been manipulated to increase the Fe concentration in the edible portions of plants. Nicotianamine (NA) is an important metal ion chelator in plants. It promotes the mobility of Fe and decreases cellular Fe toxicity. Increasing the Fe content in crops by promoting NA synthesis could help decrease human diseases associated with Fe deficiency. In the present study, Arabidopsis thaliana nicotianamine synthase 1 (AtNAS1) was overexpressed in potato (Solanum tuberosum, St) under the control of the cauliflower mosaic virus 35S promoter. Transgenic plants had a significantly increased amount of Fe in tubers (52.7 µg/g dry weight, 2.4-fold the amount in wild-type tubers), while no differences in plant phenotype or yield were detected between transgenic and wild-type plants. The expression of genes involved in root mineral uptake and homeostasis, such as StYSL1, StIRT1, StFRO1, and StNAS, was also altered in the roots and leaves of the transgenic plants. Our results demonstrate that the manipulation of Fe chelation is a useful strategy for Fe nutrition improvement, and the increased Fe accumulation in tubers of transgenic potato plants is most likely caused by the increased movement of Fe from the leaf to the tuber.
Patterns of Species Richness and Distribution of the Genus Laelia s.l. vs. Laelia s.s. (Laeliinae: Epidendroideae: Orchidaceae) in Mexico: Taxonomic Contribution and Conservation Implications
Authors: Olivares-Juárez Ma. Isabel , Burgos-Hernández Mireya , Santiago-Alvarádo Margarita
Published: October 2022

Laelia is an endemic genus of the neotropical region, with the greatest richness occurring in Mexico. A recent phylogenetic study transferred some Mexican laelias to the genus Schomburgkia, which has generated debate. The aim of the present study was to analyze the patterns of species richness and endemism and the current and potential geographic distributions of the taxa of Laelia s.l., as well as the putative Laelia s.s., distributed in Mexico as part of an exploratory evaluation of the generic limits to sheds light on the taxonomic debate and generate baselines to guide conservation efforts. A database was generated with information from herbarium specimens and publications. The species richness was estimated by political division, biomes, and elevation. The endemism was analyzed by political division and using the weighted and corrected weighted endemism indices. Geographic data, climatic, and topographic variables were used to predict the distributions with the maximum entropy algorithm. The results supported the proposal to transfer some species to the genus Schomburgkia. Some areas of the Sierra Madre del Sur and Oriental should be included as priority areas in the conservation strategies of Laelia. This study highlights the importance of the taxonomy, distribution, and hotspots in diversity conservation.
Growth and Distribution of Boron in Oilseed Rape (Brassica napus L.) as Affected by Boron Supply
Authors: Dinh Anh Quang , Naeem Asif , Mühling Karl H.
Published: October 2022

Oilseed rape (Brassica napus L.) is one of the most important oilseed crops. It has relatively high boron (B) requirements for growth. In this study, a hydroponic experiment was performed to determine the critical B requirement and B distribution in B. napus. The plants were grown for four weeks at a range of B levels (from 0.25 to 1000 µM) supplied in a nutrient solution. The results showed significant differences in the root and shoot dry matter and B accumulation in these tissues among the supplied B levels. Severe visible symptoms of B deficiency were observed on the leaves at levels lower than 1 µM B and toxicity at 1000 µM B in the nutrient solution. The maximum shoot and root dry matter were recorded at 25 µM B in the nutrient solution. The plants supplied with the lowest and the highest B levels produced 35% and 37% less shoot dry matter than those supplied with 25 µM B, while the corresponding decreases in the root dry matter were 48% and 36%, respectively. The critical concentration of B, which is the lowest concentration at which plants produce 90% of the maximum shoot dry matter, was proven to be 1 µM B for oilseed rape. At this level of external B supply, the B concentration in the shoot was 26.9 mg kg−1 DM. It was found that with the increase in B levels in the nutrient solution, the relative distribution of B between the roots and the shoots shifted in favor of the shoots.
Investigations of the Chemical Composition and Aromatic Properties of Peel Essential Oils throughout the Complete Phase of Fruit Development for Two Cultivars of Sweet Orange (Citrus sinensis (L.) Osb.)
Authors: Ferrer Vincent , Paymal Noémie , Quinton Carole , Tomi Félix , Luro François
Published: October 2022

The peel essential oil (PEO) of sweet orange is used for flavoring liquors or foods and in the perfumery and cosmetics industry. The fruit maturity stage can modify the essential oil composition and aromatic properties, but little information is available on the evolution of PEO during the entire time set of fruit development. In this study, the yield, chemical composition and aromatic profile over the three phases of orange development were monitored. Four fruit traits (peel color, weight, acidity and sweetness) were recorded to characterize fruit development. Fruits of two sweet orange cultivars were sampled every two weeks from June to May of the next year. PEO was obtained by hydrodistillation and analyzed by gas chromatography coupled with a flame ionization detector (GC-FID). Compounds were identified with GC coupled with mass spectrometry (GC/MS). Ten expert panelists using the descriptor intensity method described the aromatic profile of PEO samples. The PEO composition was richer in oxygenated compounds at early fruit development stages, with an aromatic profile presenting greener notes. During fruit growth (Phases I and II), limonene’s proportion increased considerably as a few aliphatic aldehydes brought the characteristic of orange aroma. During fruit maturation (from November to March), the PEO composition and aromatic profile were relatively stable. Later, some modifications were observed. Regardless of the fruit development stage, the two sweet oranges presented distinct PEO compositions and aromatic profiles. These results constitute a temporal reference for the chemical and aromatic evolution of sweet orange PEO in the fruit development process under Mediterranean conditions. During the first two phases of fruit development, many changes occur in the PEO composition and aroma, suggesting that their exploitation could create new products.
A New Approach to the Structure-Properties Relationships Determination for Porous Filled Reinforced Materials
Authors: Černý Miroslav , Petruš Josef , Pavliňáková Veronika
Published: October 2022

This study describes a new mathematical approach to the relationship between mechanical properties (tensile modulus, ultimate strength, and strain), composition as well as structure of porous-filled reinforced composites. The composite system consisted of a polyurethane matrix, a rubber filler, and a small amount of polyethylene terephthalate as a reinforcement. The newly proposed equations are based on a special mixing rule with the same basic form for all studied properties. The mixing rule contains a correction parameter η, which differs in different filler content in the filled part of the composite. Here, a cubic exponential function including the product of suitable structural parameters and exponents ensuring the best fitting and describable by matrix properties were successfully defined to fit the different values of correction parameter. The proposed equations should be a suitable step to obtain a relationship for describing the mechanical behavior of porous-filled and reinforced composites in the case of a small amount of reinforcement.
Processing Compostable PLA/Organoclay Bionanocomposite Foams by Supercritical CO2 Foaming for Sustainable Food Packaging
Authors: Faba Simón , Arrieta Marina P. , Agüero Ángel , Torres Alejandra , Romero Julio , Rojas Adrián , Galotto María José
Published: October 2022

This article proposes a foaming method using supercritical carbon dioxide (scCO2) to obtain compostable bionanocomposite foams based on PLA and organoclay (C30B) where this bionanocomposite was fabricated by a previous hot melt extrusion step. Neat PLA films and PLA/C30B films (1, 2, and 3 wt.%) were obtained by using a melt extrusion process followed by a film forming process obtaining films with thicknesses between 500 and 600 μm. Films were further processed into foams in a high-pressure cell with scCO2 under constant conditions of pressure (25 MPa) and temperature (130 °C) for 30 min. Bionanocomposite PLA foams evidenced a closed cell and uniform cell structure; however, neat PLA presented a poor cell structure and thick cell walls. The thermal stability was significantly enhanced in the bionanocomposite foam samples by the good dispersion of nanoclays due to scCO2, as demonstrated by X-ray diffraction analysis. The bionanocomposite foams showed improved overall mechanical performance due to well-dispersed nanoclays promoting increased interfacial adhesion with the polymeric matrix. The water uptake behavior of bionanocomposite foams showed that they practically did not absorb water during the first week of immersion in water. Finally, PLA foams were disintegrated under standard composting conditions at higher rates than PLA films, showing their sustainable character. Thus, PLA bionanocomposite foams obtained by batch supercritical foaming seem to be a sustainable option to replace non-biodegradable expanded polystyrene, and they represent a promising alternative to be considered in applications such as food packaging and other products.
Insulation Foam Concrete Nanomodified with Microsilica and Reinforced with Polypropylene Fiber for the Improvement of Characteristics
Authors: Meskhi Besarion , Beskopylny Alexey N. , Stel’makh Sergey A. , Shcherban’ Evgenii M. , Mailyan Levon R. , Beskopylny Nikita , Chernil’nik Andrei , El’shaeva Diana
Published: October 2022

Some of the primary problems of construction are brittleness and low the mechanical properties of good thermal insulation materials. Heat-insulating foam concrete has a low thermal conductivity. However, it is practically impossible to transport it over long distances since corners are cracked during transportation, the structure is broken, and, in principle, the fragility of this material is a big problem for modern buildings. The purpose of this study was to develop a heat-insulating foam concrete with improved characteristics by experimentally selecting the optimal dosage of polypropylene fiber and a nanomodifying microsilica additive. Standard methods for determining the characteristics of fiber foam concrete were used as well as the method of optical microscopy to study the structure of the composite. It has been established that the use of polypropylene fiber with the optimal reinforcement range from 1% to 3% allows us to achieve an improvement in the mechanical and physical characteristics of fiber foam concrete. The optimal dosage of the nanomodifier introduced instead of a part of the binder (10%) and polypropylene fiber (2%) by weight of the binder was determined. The maximum values of increments in mechanical characteristics were 44% for compressive strength and 73% for tensile strength in bending. The values of the thermal conductivity coefficient at optimal dosages of the nanomodifier and fiber decreased by 9%. The absence of microcracking at the phase boundary between the polypropylene fiber and the hardened cement–sand matrix due to nanomodification was noted.
Stimulus-Responsive, Gelatin-Containing Supramolecular Nanofibers as Switchable 3D Microenvironments for Cells
Authors: Hayashi Kentaro , Matsuda Mami , Nakahata Masaki , Takashima Yoshinori , Tanaka Motomu
Published: October 2022

Polymer- and/or protein-based nanofibers that promote stable cell adhesion have drawn increasing attention as well-defined models of the extracellular matrix. In this study, we fabricated two classes of stimulus-responsive fibers containing gelatin and supramolecular crosslinks to emulate the dynamic cellular microenvironment in vivo. Gelatin enabled cells to adhere without additional surface functionalization, while supramolecular crosslinks allowed for the reversible switching of the Young’s modulus through changes in the concentration of guest molecules in culture media. The first class of nanofibers was prepared by coupling the host–guest inclusion complex to gelatin before electrospinning (pre-conjugation), while the second class of nanofibers was fabricated by coupling gelatin to polyacrylamide functionalized with host or guest moieties, followed by conjugation in the electrospinning solution (post-conjugation). In situ AFM nano-indentation demonstrated the reversible switching of the Young’s modulus between 2–3 kPa and 0.2–0.3 kPa under physiological conditions by adding/removing soluble guest molecules. As the concentration of additives does not affect cell viability, the supramolecular fibers established in this study are a promising candidate for various biomedical applications, such as standardized three-dimensional culture matrices for somatic cells and the regulation of stem cell differentiation.
Effects of Co-Digestion Sludge Application on Soil Productivity
Authors: Xiao Huihuang , Chang Jianning , Xu Xin , Zhang Panyue
Published: October 2022

Anaerobic digestion and agricultural use of sewage sludge are effective methods to treat and dispose of sewage sludge, respectively. Then, the anaerobic digested sewage sludge is applied in agricultural land and the improvement of soil properties can be expected. In this study, with the purpose of evaluating the potential of co-digestion sludge for agricultural use, plot experiments with two vegetable species (radish and Chinese cabbage) and three application dosages were carried out in a short term of six months. Focus was on soil physical properties, soil nutrient change and plant growth responses during the whole process. Results showed that application of co-digestion sludge had little effect on soil physical properties, including the bulk density, porosity, capillary porosity and non-capillary porosity. However, after the application of co-digestion sludge, the maximum increase in content of organic matter, total nitrogen, hydrolysable nitrogen, total phosphorus and available phosphorus in soil reached 51%, 125%, 212%, 15% and 87%, respectively, which supplied the available nutrients quickly and continuously. The application of co-digestion sludge promoted the growth of radish and Chinese cabbage, which was observed through increase of the leaf, root biomass and plants height. Consequently, co-digestion sludge has a good application prospect for improving soil productivity as fertilizer.
Degradation of Hydroquinone Coupled with Energy Generation through Microbial Fuel Cells Energized by Organic Waste
Authors: Torlaema Tasnim Aisya Mahmuelee , Ibrahim Mohamad Nasir Mohamad , Ahmad Akil , Guerrero-Barajas Claudia , Alshammari Mohammed B. , Oh Sang-Eun , Hussain Fida
Published: October 2022

Microbial fuel cell (MFC) technology has captured the scientific community’s attention in recent years owing to its ability to directly transform organic waste into electricity through electrochemical processes. Currently, MFC systems faces a number of barriers, with one of the most significant being the lack of organic substrate to provide enough energy for bacterial growth and activity. In the current work, rotten rice was utilized as an organic substrate to boost bacterial activity to produce more energy and break down the organic pollutant hydroquinone in an effort to improve the performance of MFCs. There are only a few studies that considered the waste as an organic substrate and simultaneously degraded the organic pollutant vis-à-vis MFCs. The oxidation of glucose derived from rotten rice generated electrons that were transported to the anode surface and subsequently flowed through an external circuit to the cathode, where they were used to degrade the organic pollutant hydroquinone. The results were consistent with the MFC operation, where the 168-mV voltage was generated over the course of 29 days with a 1000 Ω external resistance. The maximum power and current densities were 1.068 mW/m2 and 123.684 mA/m2, respectively. The hydroquinone degradation was of 68%. For the degradation of organic pollutants and the production of energy, conductive pili-type bacteria such as Lacticaseibacillus, Pediococcus acidilactici and Secundilactobacillus silagincola species were identified during biological characterization. Future recommendations and concluding remarks are also included.
A Multimethod Study on Kitchen Hygiene, Consumer Knowledge and Food Handling Practices at Home
Authors: Jevšnik Mojca , Pirc Lucija , Ovca Andrej , Šantić Marina , Raspor Peter , Torkar Karmen Godič
Published: October 2022

The aim of the study was to identify consumers’ food safety knowledge, practices, and hygiene status in the observed home kitchens. The results provide the starting point for evaluating progress or regression in this area compared to the past statewide study. Food safety knowledge was analyzed among 380 consumers with an online questionnaire. Additionally, 16 consumers were observed during their preparation of specified foods. The hygiene conditions in the kitchens were microbiologically examined using contact agar plates, while the cleaning adequacy was determined by measuring the ATP bioluminescence. A lack of knowledge on certain topics regarding food safety was established; the consumers aged from 36 to 55 in general and women demonstrated the highest level of knowledge. In some cases, the observed consumers did not take proper action when preparing the food. Increased total bacteria, coliform bacteria, and Escherichia coli counts were detected in 12.7% of the consumers’ kitchens observed here. Eighty-three (74.1%) out of 112 surfaces examined with either hygiene test sheets or ATP swabs met the standards and were adequately or acceptably cleaned. The kitchen surfaces exceeded the recommended limits for 25% of consumers. Statistical differences in RLU and TCC levels on surfaces between older and younger consumers were not observed, although all (25%) inadequately cleaned kitchens belonged to older consumers. The greatest emphasis has to be put on the cleaning of home kitchens and personal hygiene. Even though consumers have some knowledge on food safety, they often fail to put that knowledge into daily practice.
A Study on Spatial-Temporal Differentiation and Influencing Factors of Agricultural Water Footprint in the Main Grain-Producing Areas in China
Authors: Wang Yun , Su Zhaoxian , Zhang Qingqing
Published: October 2022

It is an urgent scientific issue to explore the spatial and temporal differentiation and impact indicators of the agricultural water footprint in major grain-producing areas. Therefore, this study tries to use the water footprint theory to implement top-down calculation of the agricultural water footprint in major grain-producing regions from 2000 to 2019 and investigate the various impacts on the agricultural water footprint under the influence of spatial-temporal effects using spatial autocorrelation and the spatial Dubin model. The results indicate that from 2000 to 2019, the overall agricultural water footprint of China showed a fluctuating downward trend in an inverted N shape and demonstrated high–high and low–low spatial aggregation characteristics. There are notable characteristics, including high spatial dependence, spatial barriers, and path locking of the agricultural water footprint in most provinces and regions of the main grain-producing areas. Policy factors, water-saving technologies, social development, economic development, and industrial structure adjustment are all significantly and negatively correlated with the increase in the agricultural water footprint, while agricultural production and natural factors have a significant positive relationship with the agricultural water footprint. The spatial spillover effect of water-saving technologies, industrial restructuring, agricultural production, and natural factors is powerful. Therefore, a rationally optimized industrial structure, strengthened regional linkage of water resources management and control, and the promotion of efficient water infrastructure technology are important ways to inhibit the agricultural water footprint.