bluuebunny/crossref_metadata_2025 huggingface dataset:
Optimization of Protein Extraction Method for 2DE Proteomics of Goat’s Milk
Authors: Mansor Muzammeer , Al-Obaidi Jameel R. , Jaafar Nurain Nadiah , Ismail Intan Hakimah , Zakaria Atiqah Farah , Abidin Mohd Azri Zainal , Selamat Jinap , Radu Son , Jambari Nuzul Noorahya
Published: June 2020

Two-dimensional electrophoretic (2DE)-based proteomics remains a powerful tool for allergenomic analysis of goat’s milk but requires effective extraction of proteins to accurately profile the overall causative allergens. However, there are several current issues with goat’s milk allergenomic analysis, and among these are the absence of established standardized extraction method for goat’s milk proteomes and the complexity of goat’s milk matrix that may hamper the efficacy of protein extraction. This study aimed to evaluate the efficacies of three different protein extraction methods, qualitatively and quantitatively, for the 2DE-proteomics, using milk from two commercial dairy goats in Malaysia, Saanen, and Jamnapari. Goat’s milk samples from both breeds were extracted by using three different methods: a milk dilution in urea/thiourea based buffer (Method A), a triphasic separation protocol in methanol/chloroform solution (Method B), and a dilution in sulfite-based buffer (Method C). The efficacies of the extraction methods were assessed further by performing the protein concentration assay and 1D and 2D SDS-PAGE profiling, as well as identifying proteins by MALDI-TOF/TOF MS/MS. The results showed that method A recovered the highest amount of proteins (72.68% for Saanen and 71.25% for Jamnapari) and produced the highest number of protein spots (199 ± 16.1 and 267 ± 10.6 total spots for Saanen and Jamnapari, respectively) with superior gel resolution and minimal streaking. Six milk protein spots from both breeds were identified based on the positive peptide mass fingerprinting matches with ruminant milk proteins from public databases, using the Mascot software. These results attest to the fitness of the optimized protein extraction protocol, method A, for 2DE proteomic and future allergenomic analysis of the goat’s milk.
Antiviral Effects of Plant-Derived Essential Oils and Their Components: An Updated Review
Authors: Ma Li , Yao Lei
Published: June 2020

The presence of resistance to available antivirals calls for the development of novel therapeutic agents. Plant-derived essential oils may serve as alternative sources of virus-induced disease therapy. Previous studies have demonstrated essential oils to be excellent candidates to treat antiviral-resistant infection associated with their chemical complexity which confers broad-spectrum mechanisms of action and non-specific antiviral properties. However, almost no comprehensive reviews are updated to generalize knowledge in this regard and disclose the interplay between the components and their antiviral activities. This review provides an up-to-date overview of the antiviral efficacy of essential oils from a wide range of plant species and their characteristic components, as well as their overall mechanisms of action, focusing on the last decade. The roles of individual components relative to the overall antiviral efficacy of essential oils, together with the antiviral activity of essential oils in comparison with commercial drugs are also discussed. Lastly, the inadequacies in current research and future research are put forward. This review will provide references in the design of new drug prototypes and improve our understanding of the proper applications of essential oils in the future.
Encapsidation of Different Plasmonic Gold Nanoparticles by the CCMV CP
Authors: Durán-Meza Ana L. , Escamilla-Ruiz Martha I. , Segovia-González Xochitl F. , Villagrana-Escareño Maria V. , Vega-Acosta J. Roger , Ruiz-Garcia Jaime
Published: June 2020

Different types of gold nanoparticles have been synthesized that show great potential in medical applications such as medical imaging, bio-analytical sensing and photothermal cancer therapy. However, their stability, polydispersity and biocompatibility are major issues of concern. For example, the synthesis of gold nanorods, obtained through the elongated micelle process, produce them with a high positive surface charge that is cytotoxic, while gold nanoshells are unstable and break down in a few weeks due to the Ostwald ripening process. In this work, we report the self-assembly of the capsid protein (CP) of cowpea chlorotic mottle virus (CCMV) around spherical gold nanoparticles, gold nanorods and gold nanoshells to form virus-like particles (VLPs). All gold nanoparticles were synthesized or treated to give them a negative surface charge, so they can interact with the positive N-terminus of the CP leading to the formation of the VLPs. To induce the protein self-assembly around the negative gold nanoparticles, we use different pH and ionic strength conditions determined from a CP phase diagram. The encapsidation with the viral CP will provide the nanoparticles better biocompatibility, stability, monodispersity and a new biological substrate on which can be introduced ligands toward specific cells, broadening the possibilities for medical applications.
Characteristics of Panax ginseng Cultivars in Korea and China
Authors: Zhang Hao , Abid Suleman , Ahn Jong Chan , Mathiyalagan Ramya , Kim Yu-Jin , Yang Deok-Chun , Wang Yingping
Published: June 2020

Ginseng (Panax ginseng Meyer) is one of the most important medicinal herbs in Asia. Its pharmacological activity comes from ginsenosides, and its roots are produced commercially for traditional and Oriental medicine. Though 17 Panax species are available around the world, there was a need to develop cultivars adapted to different climatic conditions and resistant to various diseases while still producing high-quality, high-yield roots. Thus, 12 and 9 commercial P. ginseng cultivars have been registered in South Korea and China, respectively. Those varieties show superiority to local landraces. For example, Chunpoong is more highly resistant to rusty rot disease than the local Jakyungjong landrace and has a good root shape; it is highly cultivated to produce red ginseng. The Chinese cultivar Jilin Huangguo Renshen has higher ginsenoside content than its local landraces. This review provides information about P. ginseng cultivars and offers directions for future research, such as intra- and interspecific hybridization.
Guanine Deaminase in Human Epidermal Keratinocytes Contributes to Skin Pigmentation
Authors: Jung Joon Min , Noh Tai Kyung , Jo Soo Youn , Kim Su Yeon , Song Youngsup , Kim Young-Hoon , Chang Sung Eun
Published: June 2020

Epidermal keratinocytes are considered as the most important neighboring cells that modify melanogenesis. Our previous study used microarray to show that guanine deaminase (GDA) gene expression is highly increased in melasma lesions. Hence, we investigated the role of GDA in skin pigmentation. We examined GDA expression in post-inflammatory hyperpigmentation (PIH) lesions, diagnosed as Riehl’s melanosis. We further investigated the possible role of keratinocyte-derived GDA in melanogenesis by quantitative PCR, immunofluorescence staining, small interfering RNA-based GDA knockdown, and adenovirus-mediated GDA overexpression. We found higher GDA positivity in the hyperpigmentary lesional epidermis than in the perilesional epidermis. Both UVB irradiation and stem cell factor (SCF) plus endothelin-1 (ET-1) were used, which are well-known melanogenic stimuli upregulating GDA expression in both keratinocyte culture alone and keratinocyte and melanocyte coculture. GDA knockdown downregulated melanin content, while GDA overexpression promoted melanogenesis in the coculture. When melanocytes were treated with UVB-exposed keratinocyte-conditioned media, the melanin content was increased. Also, GDA knockdown lowered SCF and ET-1 expression levels in keratinocytes. GDA in epidermal keratinocytes may promote melanogenesis by upregulating SCF and ET-1, suggesting its role in skin hyperpigmentary disorders.
Recent Advances in π-Conjugated N^C-Chelate Organoboron Materials
Authors: Haque Ashanul , Al-Balushi Rayya A. , Raithby Paul R. , Khan Muhammad S.
Published: June 2020

Boron-containing π-conjugated materials are archetypical candidates for a variety of molecular scale applications. The incorporation of boron into the π-conjugated frameworks significantly modifies the nature of the parent π-conjugated systems. Several novel boron-bridged π-conjugated materials with intriguing structural, photo-physical and electrochemical properties have been reported over the last few years. In this paper, we review the properties and multi-dimensional applications of the boron-bridged fused-ring π-conjugated systems. We critically highlight the properties of π-conjugated N^C-chelate organoboron materials. This is followed by a discussion on the potential applications of the new materials in opto-electronics (O-E) and other areas. Finally, attempts will be made to predict the future direction/outlook for this class of materials.
Simultaneous Adsorption of Malachite Green and Methylene Blue Dyes in a Fixed-Bed Column Using Poly(Acrylonitrile-Co-Acrylic Acid) Modified with Thiourea
Authors: Adeyi Abel Adekanmi , Jamil Siti Nurul Ain Md , Abdullah Luqman Chuah , Choong Thomas Shean Yaw , Lau Kia Li , Alias Nor Halaliza
Published: June 2020

Proper remediation of aquatic environments contaminated by toxic organic dyes has become a research focus globally for environmental and chemical engineers. This study evaluates the adsorption potential of a polymer-based adsorbent, thiourea-modified poly(acrylonitrile-co-acrylic acid) (T-PAA) adsorbent, for the simultaneous uptake of malachite green (MG) and methylene blue (MB) dye ions from binary system in a continuous flow adsorption column. The influence of inlet dye concentrations, pH, flow rate, and adsorbent bed depth on adsorption process were investigated, and the breakthrough curves obtained experimentally. Results revealed that the sorption capacity of the T-PAA for MG and MB increase at high pH, concentration and bed-depth. Thomas, Bohart-Adams, and Yoon-Nelson models constants were calculated to describe MG and MB adsorption. It was found that the three dynamic models perfectly simulate the adsorption rate and behavior of cationic dyes entrapment. Finally, T-PAA adsorbent demonstrated good cyclic stability. It can be regenerated seven times (or cycles) with no significant loss in adsorption potential. Overall, the excellent sorption capacity and multiple usage make T-PAA polymer an attractive adsorbent materials for treatment of multicomponent dye bearing effluent in a fixed-bed column system.
Cryptococcus neoformans Capsular GXM Conformation and Epitope Presentation: A Molecular Modelling Study
Authors: Kuttel Michelle M. , Casadevall Arturo , Oscarson Stefan
Published: June 2020

The pathogenic encapsulated Cryptococcus neoformans fungus causes serious disease in immunosuppressed hosts. The capsule, a key virulence factor, consists primarily of the glucuronoxylomannan polysaccharide (GXM) that varies in composition according to serotype. While GXM is a potential vaccine target, vaccine development has been confounded by the existence of epitopes that elicit non-protective antibodies. Although there is evidence for protective antibodies binding conformational epitopes, the secondary structure of GXM remains an unsolved problem. Here an array of molecular dynamics simulations reveal that the GXM mannan backbone is consistently extended and relatively inflexible in both C. neoformans serotypes A and D. Backbone substitution does not alter the secondary structure, but rather adds structural motifs: β DGlcA and β DXyl side chains decorate the mannan backbone in two hydrophillic fringes, with mannose-6-O-acetylation forming a hydrophobic ridge between them. This work provides mechanistic rationales for clinical observations—the importance of O-acetylation for antibody binding; the lack of binding of protective antibodies to short GXM fragments; the existence of epitopes that elicit non-protective antibodies; and the self-aggregation of GXM chains—indicating that molecular modelling can play a role in the rational design of conjugate vaccines.
Dendrimer-Functionalized Hybrid Materials Based on Silica as Novel Carriers of Bioactive Acids
Authors: Pawlaczyk Mateusz , Schroeder Grzegorz
Published: June 2020

One of the major goals in the materials science is the design and development of non-toxic, versatile, and efficient drug delivery systems. The study reported in this paper concerns the syntheses of poly(amidoamine) (PAMAM) dendrimers with tris(2-aminoethyl)amine as an amine core and different terminal amines, and their attachment to silica matrix. The obtained ethylenediamine (EDA), triethylenetetramine (TETA), tris(2-aminoethyl)amine (TREN) and 4,7,10-trioxa-1,13-tridecanediamine (TRI-OXA) dendrimers were introduced to the support surface via an epoxy linker, leading to a loading efficiency in the range of 0.054–0.113 mmol g−1, determined using elemental and thermogravimetric analyses. The materials exhibited high adsorption capacities towards the chosen model drugs: folic, salicylic and nicotinic acid. The investigated adsorption processes were found to follow the Freundlich isotherm model, with indication of the drugs’ structure influence on the binding efficiency. Drug-loaded hybrid materials were also described for in vitro drug release in three pH-different paraphysiological media. The highest percentage release was obtained in the tests performed at pH 2.0, ranging between 35.42 and 99.83%. Satisfactory results and the versatility of PAMAM dendrimers may lead to the application of such materials not only as drug carriers dedicated to a wide range of pharmaceutics, but also as analytical tools for pre-concentration and/or the determination of biocompound contamination in samples.
Immobilization of Purified Pectin Lyase from Pseudomonas putida onto Magnetic Lily Flowers (Lilium candidum L.) Nanoparticles and Applicability in Industrial Processes
Authors: Tasgin Esen , Nadaroglu Hayrunnisa , Babagil Aynur , Demir Nazan
Published: June 2020

Pectinases are an important class of enzymes distributed in many higher plants and microorganisms. One of these enzymes is pectin lyase which has an important role in industrial applications such as clarification of fruit juices. Pectin lyase was purified with 73% yield from Pseudomonas putida bacteria and was 220.7-fold using three phase precipitation technique. Molecular weight of purified pectin lyase was determined as 32.88 kDa with SDS-polyacrylamide gel electrophoresis. The pectin lyase was immobilized covalently via the L-glutaraldehyde spacer to the cellulosic structures of lily flowers (Lilium candidum L.). The immobilized enzyme was then magnetized by modifying with γ-Fe3O4 nanoparticles and determined the most appropriate immobilization conditions as pH 6 and 30 °C. Purified pectin lyase was connected to magnetized support material after 60 min at the rate of 86.4%. The optimum pH and temperatures for the free and immobilized pectin lyase was found to be 6.0 and 40 °C. pH and thermal stabilities of the free and immobilized pectin lyase enzyme have been preserved at high-low temperatures and pH. The structural characterization of the immobilized pectin lyase was performed by SEM, FT-IR, and XRD chromatographic analyses and it was observed that the support materials structure was appropriated to immobilization with pectin lyase and to modify with Fe3O4 nanoparticles.
Exploring Smart Agents for the Interaction with Multimodal Mediated Environments
Authors: Richer Robert , Zhao Nan , Eskofier Bjoern M. , Paradiso Joseph A.
Published: June 2020

After conversational agents have been made available to the broader public, we speculate that applying them as a mediator for adaptive environments reduces control complexity and increases user experience by providing a more natural interaction. We implemented and tested four agents, each of them differing in their system intelligence and input modality, as personal assistants for Mediated Atmospheres, an adaptive smart office prototype. They were evaluated in a user study ( N = 33 ) to collect subjective and objective measures. Results showed that a smartphone application was the most favorable system, followed by conversational text and voice agents that were perceived as being more engaging and intelligent than a non-conversational voice agent. Significant differences were observed between native and non-native speakers in both subjective and objective measures. Our findings reveal the potential of conversational agents for the interaction with adaptive environments to reduce work and information overload.
Design of Novel Perovskite-Based Polymeric Poly(l-Lactide-Co-Glycolide) Nanofibers with Anti-Microbial Properties for Tissue Engineering
Authors: Góra Aleksander , Tian Lingling , Ramakrishna Seeram , Mukherjee Shayanti
Published: June 2020

There is a growing need for anti-microbial materials in several biomedical application areas, such are hernia, skin grafts as well as gynecological products, owing to the complications caused by infection due to surgical biomaterials. The anti-microbial effects of silver in the form of nanoparticles, although effective, can be toxic to surrounding cells. In this study, we report, for the first time, a novel biomedical application of Ag0.3Na1.7La2Ti3O10-layered perovskite particles, blended with poly(L-lactide-co-glycolide) (PLGA), aimed at designing anti-microbial and tissue engineering scaffolds. The perovskite was incorporated in three concentrations of 1, 5, 10 and 15 w/w% and electrospun using dimethylformamide (DMF) and chloroform. The morphology of the resultant nanofibers revealed fiber diameters in the range of 408 to 610 nm by scanning electron microscopy. Mechanical properties of perovskite-based nanofibers also matched similar mechanical properties to human skin. We observed impressive anti-microbial activity, against Gram-negative, Gram-positive bacteria and even fungi, to Ag0.3Na1.7La2Ti3O10 in powder as well as nanofiber-incorporated forms. Furthermore, cytotoxicity assay and immunocytochemistry revealed that perovskite-based nanofibers promoted the proliferation of human dermal fibroblasts whist maintaining normal cellular protein expression. Our study shows that perovskite-nanofibers have potential as scaffolds for biomedical applications with anti-microbial needs.
Solution-Based Deposition of Transparent Eu-Doped Titanium Oxide Thin Films for Potential Security Labeling and UV Screening
Authors: Molkenova Anara , Khamkhash Laura , Zhussupbekova Ainur , Zhussupbekov Kuanysh , Sarsenov Sagyntay , Taniguchi Izumi , Shvets Igor V. , Atabaev Timur Sh.
Published: June 2020

Transparent titanium oxide thin films attract enormous attention from the scientific community because of their prominent properties, such as low-cost, chemical stability, and optical transparency in the visible region. In this study, we developed an easy and scalable solution-based process for the deposition of transparent TiOx thin films on glass substrates. We showed that the proposed method is also suitable for the fabrication of metal-doped TiOx thin films. As proof-of-the-concept, europium Eu(III) ions were introduced into TiOx film. A photoluminescence (PL) study revealed that Eu-doped TiOx thin films showed strong red luminescence associated with 5D0→7Fj relaxation transitions in Eu (III). We found that prepared TiOx thin films significantly reduce the transmittance of destructive UV radiation; a feature that can be useful for the protection of photovoltaic devices. In addition, transparent and luminescent TiOx thin films can be utilized for potential security labeling.
Engineering Metal–Organic Frameworks (MOFs) for Controlled Delivery of Physiological Gaseous Transmitters
Authors: Zhang Mengdan , Qiao Ruirui , Hu Jinming
Published: June 2020

Metal–organic frameworks (MOFs) comprising metal ions or clusters coordinated to organic ligands have become a class of emerging materials in the field of biomedical research due to their bespoke compositions, highly porous nanostructures, large surface areas, good biocompatibility, etc. So far, many MOFs have been developed for imaging and therapy purposes. The unique porous nanostructures render it possible to adsorb and store various substances, especially for gaseous molecules, which is rather challenging for other types of delivery vectors. In this review, we mainly focus on the recent development of MOFs for controlled release of three gaseous transmitters, namely, nitric oxide (NO), carbon monoxide (CO), and hydrogen sulfide (H2S). Although these gaseous molecules have been known as air pollutants for a long time, much evidence has been uncovered regarding their important physiological functions as signaling molecules. These signaling molecules could be either physically absorbed onto or covalently linked to MOFs, allowing for the release of loaded signaling molecules in a spontaneous or controlled manner. We highlight the designing concept by selective examples and display their potential applications in many fields such as cancer therapy, wound healing, and anti-inflammation. We hope more effort could be devoted to this emerging fields to develop signaling molecule-releasing MOFs with practical applications.
Association of Dietary Patterns and Type-2 Diabetes Mellitus in Metabolically Homogeneous Subgroups in the KORA FF4 Study
Authors: Wawro Nina , Pestoni Giulia , Riedl Anna , Breuninger Taylor A. , Peters Annette , Rathmann Wolfgang , Koenig Wolfgang , Huth Cornelia , Meisinger Christa , Rohrmann Sabine , Linseisen Jakob
Published: June 2020

There is evidence that a change in lifestyle, especially physical activity and diet, can reduce the risk of developing type-2 diabetes mellitus (T2DM). However, the response to dietary changes varies among individuals due to differences in metabolic characteristics. Therefore, we investigated the association between dietary patterns and T2DM while taking into account these differences. For 1287 participants of the population-based KORA FF4 study (Cooperative Health Research in the Region of Augsburg), we identified three metabolically-homogenous subgroups (metabotypes) using 16 clinical markers. Based on usual dietary intake data, two diet quality scores, the Mediterranean Diet Score (MDS) and the Alternate Healthy Eating Index (AHEI), were calculated. We explored the associations between T2DM and diet quality scores. Multi-variable adjusted models, including metabotype subgroup, were fitted. In addition, analyses stratified by metabotype were carried out. We found significant interaction effects between metabotype and both diet quality scores (p < 0.05). In the analysis stratified by metabotype, significant negative associations between T2DM and both diet quality scores were detected only in the metabolically-unfavorable homogenous subgroup (Odds Ratio (OR) = 0.62, 95% confidence interval (CI) = 0.39–0.90 for AHEI and OR = 0.60, 95% CI = 0.40–0.96 for MDS). Prospective studies taking metabotype into account are needed to confirm our results, which allow for the tailoring of dietary recommendations in the prevention of T2DM.
Semi-Solid Nutrients for Prevention of Enteral Tube Feeding-Related Complications in Japanese Population: A Systematic Review and Meta-Analysis
Authors: Kokura Yoji , Suzuki Chieko , Wakabayashi Hidetaka , Maeda Keisuke , Sakai Kotomi , Momosaki Ryo
Published: June 2020

The aim of this systematic review was to assess the best available evidence on semi-solid nutrients for prevention of complications associated with enteral tube feeding (ETF). PubMed (MEDLINE), EMBASE, Cochrane Central Register of Controlled Trial, Ichushi-web, and World Health Organization International Clinical Trials Registry Platform databases were searched for relevant articles. Randomized controlled trials (RCTs), cluster RCTs, and crossover trials comparing the effects of semi-solid nutrients with those of control interventions in patients on ETF were included in the review. The primary outcome was development of gastroesophageal reflux (GER). Eight RCTs and five crossover trials involving 889 study participants in total were examined via meta-analysis. The meta-analysis showed that semi-solid nutrients significantly decreased the risk of GER (risk ratio 0.39; 95% confidence interval (CI) 0.21 to 0.73) and the GER index (mean difference −2.93; 95% CI −5.18 to −0.68). Dwell time in the stomach was significantly shortened (standardized mean difference (SMD) −0.50; 95% CI −0.99 to −0.02), as was care time defined as the time needed to prepare and administer the nutrient solution (SMD −8.02; 95% CI −10.94 to −5.10). Semi-solid nutrients significantly decrease the risk of GER and the dwell time in the stomach in adult patients.
Reformulation of Pastry Products to Improve Effects on Health
Authors: Estruch Ramon , Vendrell Eulàlia , Ruiz-León Ana María , Casas Rosa , Castro-Barquero Sara , Alvarez Xavier
Published: June 2020

Obesity is increasing at an alarming rate and has been described as a global pandemic. This increase has several explanations, including an increase in caloric intake, low levels of physical activity and the nutritional composition of our diets. In addition to public health policies based on healthy dietary patterns and recommendations based on the Mediterranean and other healthy diets, food reformulation, especially of commonly consumed processed foods, such as bakery products and pastries, is needed in the fight against obesity. Among nutritional reformulation strategies, reductions in caloric density, salt, added sugar, saturated and trans-fats are important in order to reduce the associated risk of developing chronic diseases, including cardiovascular diseases, diabetes and cancer.
Diet Supplementation, Probiotics, and Nutraceuticals in SARS-CoV-2 Infection: A Scoping Review
Authors: Infusino Fabio , Marazzato Massimiliano , Mancone Massimo , Fedele Francesco , Mastroianni Claudio Maria , Severino Paolo , Ceccarelli Giancarlo , Santinelli Letizia , Cavarretta Elena , Marullo Antonino G. M. , Miraldi Fabio , Carnevale Roberto , Nocella Cristina , Biondi-Zoccai Giuseppe , Pagnini Cristiano , Schiavon Sonia , Pugliese Francesco , Frati Giacomo , d’Ettorre Gabriella
Published: June 2020

The severe acute respiratory syndrome coronavirus 2 (Sars-CoV-2) global pandemic is a devastating event that is causing thousands of victims every day around the world. One of the main reasons of the great impact of coronavirus disease 2019 (COVID-19) on society is its unexpected spread, which has not allowed an adequate preparation. The scientific community is fighting against time for the production of a vaccine, but it is difficult to place a safe and effective product on the market as fast as the virus is spreading. Similarly, for drugs that can directly interfere with viral pathways, their production times are long, despite the great efforts made. For these reasons, we analyzed the possible role of non-pharmacological substances such as supplements, probiotics, and nutraceuticals in reducing the risk of Sars-CoV-2 infection or mitigating the symptoms of COVID-19. These substances could have numerous advantages in the current circumstances, are generally easily available, and have negligible side effects if administered at the already used and tested dosages. Large scientific evidence supports the benefits that some bacterial and molecular products may exert on the immune response to respiratory viruses. These could also have a regulatory role in systemic inflammation or endothelial damage, which are two crucial aspects of COVID-19. However, there are no specific data available, and rigorous clinical trials should be conducted to confirm the putative benefits of diet supplementation, probiotics, and nutraceuticals in the current pandemic.
Lutein Supplementation for Eye Diseases
Authors: Li Long Hin , Lee Jetty Chung-Yung , Leung Ho Hang , Lam Wai Ching , Fu Zhongjie , Lo Amy Cheuk Yin
Published: June 2020

Lutein is one of the few xanthophyll carotenoids that is found in high concentration in the macula of human retina. As de novo synthesis of lutein within the human body is impossible, lutein can only be obtained from diet. It is a natural substance abundant in egg yolk and dark green leafy vegetables. Many basic and clinical studies have reported lutein’s anti-oxidative and anti-inflammatory properties in the eye, suggesting its beneficial effects on protection and alleviation of ocular diseases such as age-related macular degeneration, diabetic retinopathy, retinopathy of prematurity, myopia, and cataract. Most importantly, lutein is categorized as Generally Regarded as Safe (GRAS), posing minimal side-effects upon long term consumption. In this review, we will discuss the chemical structure and properties of lutein as well as its application and safety as a nutritional supplement. Finally, the effects of lutein consumption on the aforementioned eye diseases will be reviewed.
Extra Virgin Olive Oil Phenol Extracts Exert Hypocholesterolemic Effects through the Modulation of the LDLR Pathway: In Vitro and Cellular Mechanism of Action Elucidation
Authors: Lammi Carmen , Bellumori Maria , Cecchi Lorenzo , Bartolomei Martina , Bollati Carlotta , Clodoveo Maria Lisa , Corbo Filomena , Arnoldi Anna , Mulinacci Nadia
Published: June 2020

This study was aimed at investigating the hypocholesterolemic effects of extra virgin olive oil (EVOO) phenols and the mechanisms behind the effect. Two phenolic extracts were prepared from EVOO of different cultivars and analyzed using the International Olive Council (IOC) official method for total phenols, a recently validated hydrolytic procedure for total hydroxytyrosol and tyrosol, and 1H-NMR analysis in order to assess their secoiridoid profiles. Both of the extracts inhibited in vitro the 3-hydroxy-3-methylglutaryl co-enzyme A reductase (HMGCoAR) activity in a dose-dependent manner. After the treatment of human hepatic HepG2 cells (25 µg/mL), they increased the low-density lipoprotein (LDL) receptor protein levels through the activation of the sterol regulatory element binding proteins (SREBP)-2 transcription factor, leading to a better ability of HepG2 cells to uptake extracellular LDL molecules with a final hypocholesterolemic effect. Moreover, both of the extracts regulated the intracellular HMGCoAR activity through the increase of its phosphorylation by the activation of AMP-activated protein kinase (AMPK)-pathways. Unlike pravastatin, they did not produce any unfavorable effect on proprotein convertase subtilisin/kexin 9 (PCSK9) protein level. Finally, the fact that extracts with different secoiridoid profiles induce practically the same biological effects suggests that the hydroxytyrosol and tyrosol derivatives may have similar roles in hypocholesterolemic activity.
Destigmatizing Carbohydrate with Food Labeling: The Use of Non-Mandatory Labelling to Highlight Quality Carbohydrate Foods
Authors: Marinangeli Christopher P.F. , Harding Scott V. , Glenn Andrea J. , Chiavaroli Laura , Zurbau Andreea , Jenkins David J.A. , Kendall Cyril W.C. , Miller Kevin B. , Sievenpiper John L.
Published: June 2020

Dietary carbohydrates are components of healthy foods, but many carbohydrate foods have recently been stigmatized as primary causes of diet-related risk factors for chronic disease. There is an opportunity to enhance efforts within the food landscape to encourage the consumption of higher quality carbohydrate foods. The use of labelling is one strategy that permits consumers to identify healthy carbohydrate foods at the point-of-purchase. This review discusses the regulatory frameworks and examples of associated non-mandatory food labelling claims that are currently employed to highlight healthy carbohydrate foods to consumers. The existing labelling frameworks discussed here align with established measures of carbohydrate quality, such as 1. dietary fibre nutrient content claims and associated dietary fibre-based health claims; 2. the presence of whole carbohydrate foods and ingredients that are intact or reconstituted, such as whole grains; and 3. low glycemic index and glycemic response claims. Standards from Codex Alimentarius, and regulations from Australia and New Zealand, Canada, Europe, and the United States will be used to illustrate the means by which food labelling can be used by consumers to identify quality carbohydrate foods.
The Footprints of the Wreckage of the Italian Royal Navy Battleship Leonardo Da Vinci on the Mar Piccolo Sea-Bottom (Taranto, Southern Italy)
Authors: Scardino Giovanni , De Giosa Francesco , D’Onghia Marco , Demonte Pietro , Fago Paola , Saccotelli Gianluca , Valenzano Eliana , Moretti Massimo , Velardo Raffaele , Capasso Gennaro , Corbelli Vera , Mastronuzzi Giuseppe
Published: June 2020

Over the last two decades, the scientific interest for marine geophysical surveys has shown a huge increase, mostly for multidisciplinary applicability on different studies, from historical heritage to environmental remediation. One of the main research fields carried out through marine geophysical surveys is the study of the wreckage footprints connected to the seabed perturbation. In 2018, a strategic project planned by the Italian National Government Commissioner for the remediation in the Taranto area (Southern Italy) stands as a basic sample for such issues. The project aimed at the detection of anthropogenic impact in the highly polluted Mar Piccolo and Mar Grande basins seabed, through a multidisciplinary approach involving geological, biological, chemical, engineering, and ecological studies. The main purpose of the work was to identify any potential pollution source, focusing on anthropogenic sea-floor features such as uncontrolled dumping, wrecks, or other objects of peculiar origin. To achieve the purpose of the work, field surveys were planned and performed in order to direct a general policy and accurate planning for environmental remediation activities. Different marine geophysics methods were used to characterize the main sea-floor features and to detect each anthropogenic feature. A comparative analysis of a high-resolution dataset allowed to clarify the origin of some deep depressions on the Mar Piccolo sea-bottom, which at the first instance were associated with a natural origin, as the results of the Leonardo Da Vinci wreckage and related recovering activities. High-resolution morphobathymetric, magnetometric, and seismic data revealed the story of the Italian Royal Navy battleship which sunk on 2 August 1916 as a slight footprint on the Mar Piccolo seabed but a deep historical heritage of the city of Taranto. Moreover, final results demonstrate high-resolution marine survey methodologies’ complete applicability to environmental, historical, and scientific issues.
Elucidation of Inverse Agonist Activity of Bilastine
Authors: Mizuguchi Hiroyuki , Wakugawa Tomoharu , Sadakata Hisato , Kamimura Seiichiro , Takemoto Mai , Nakagawa Tomomi , Yabumoto Masami , Kitamura Yoshiaki , Takeda Noriaki , Fukui Hiroyuki
Published: June 2020

H1-antihistamines antagonize histamine and prevent it from binding to the histamine H1 receptor (H1R). Some of them also act as inverse agonists, which are more potent than pure antagonists because they suppress the constitutive H1R activity. Bilastine is a non-sedative antihistamine which is one of the most satisfy the requirements for oral antihistamines. However, there is no information to show the inverse agonist activity of bilastine including inositol phosphates accumulation, and its inverse agonist activity is yet to be elucidated. Here we evaluated whether bilastine has inverse agonist activity or not. Intracellular calcium concentration was measured using Fluo-8. Inositol phosphates accumulation was assayed using [3H]myo-inositol. The H1R mRNA level was measured using real-time RT-PCR. At rest, Ca2+ oscillation was observed, indicating that H1R has intrinsic activity. Bilastine attenuated this fluorescence oscillation. Bilastine suppressed the increase in IPs formation in a dose-dependent manner and it was about 80% of the control level at the dose of 3 μM. Bilastine also suppressed histamine-induced increase in IPs formation to the control level. Furthermore, bilastine suppressed basal H1R gene expression in a dose-dependent manner. Data suggest that bilastine is an inverse agonist. Preseasonal prophylactic administration with bilastine could down-regulate basal H1R gene expression in the nasal mucosa and ameliorate the nasal symptoms during the peak pollen period.
Environmentally Efficient 316L Stainless Steel Feedstocks for Powder Injection Molding
Authors: Hausnerova Berenika , Novak Martin
Published: June 2020

In this study, environmentally convenient highly metal powder filled feedstocks intended for powder injection molding is presented. The composition of 60 vol % 316L stainless steel gas atomized powder feedstocks containing semicrystalline waxes: acrawax or carnauba wax and paraffin wax, combined with polyethylene glycol and modifier, was optimized to provide defect-free parts. Rheological as well as thermogravimetric analyses supported with scanning electron microscopy and metallography were employed to set up optimum conditions for molding, debinding and sintering. The performance of the novel feedstock was compared with currently available polyolefines-based materials, and results showed an efficiency enhancement due to the substantially lower (about 100 °C) mixing and molding temperatures as well as a reduction of debinding and sintering times at the simultaneous achievement of better mechanical properties in terms of elongation and tensile strength, in comparison to the mass production feedstock.
Introducing the Newly Isolated Bacterium Aneurinibacillus sp. H1 as an Auspicious Thermophilic Producer of Various Polyhydroxyalkanoates (PHA) Copolymers–2. Material Study on the Produced Copolymers
Authors: Sedlacek Petr , Pernicova Iva , Novackova Ivana , Kourilova Xenie , Kalina Michal , Kovalcik Adriana , Koller Martin , Nebesarova Jana , Krzyzanek Vladislav , Hrubanova Kamila , Masilko Jiri , Slaninova Eva , Trudicova Monika , Obruca Stanislav
Published: June 2020

Aneurinibacillus sp. H1 is a promising, moderately thermophilic, novel Gram-positive bacterium capable of the biosynthesis of polyhydroxyalkanoates (PHA) with tunable monomer composition. In particular, the strain is able to synthesize copolymers of 3-hydroxybutyrate (3HB), 4-hydroxybutyrate (4HB) and 3-hydroxyvalerate (3HV) with remarkably high 4HB and 3HV fractions. In this study we performed an in-depth material analysis of PHA polymers produced by Aneurinibacillus sp. H1 in order to describe how the monomer composition affects fundamental structural and physicochemical parameters of the materials in the form of solvent-casted films. Results of infrared spectroscopy, X-ray diffractometry and thermal analysis clearly show that controlling the monomer composition enables optimization of PHA crystallinity both qualitatively (the type of the crystalline lattice) and quantitatively (the overall degree of crystallinity). Furthermore, resistance of the films against thermal and/or enzymatic degradation can also be manipulated by the monomer composition. Results of this study hence confirm Aneurinibacillus sp. H1 as an auspicious candidate for thermophilic production of PHA polymers with material properties that can be tuned together with their chemical composition by the corresponding adjustment of the cultivation process.
Influence of Reduced Graphene Oxide on the Electropolymerization of 5-Amino-1-naphthol and the Interaction of 1,4-Phenylene Diisothiocyanate with the Poly(5-Amino-1-naphtol)/Reduced Graphene Oxide Composite
Authors: Baibarac Mihaela , Daescu Monica , Socol Marcela , Bartha Cristina , Negrila Cătălin , Fejer Szilárd N.
Published: June 2020

A new composite base on reduced graphene oxide (RGO) and poly(5-amino-1-naphthol) (P5A1N) was synthesized by the electrochemical polymerization of 5-amino-1-naphthol (5A1N) in the presence of HClO4 and H4SiW12O40 onto the surface of Au electrode covered with the RGO sheets. The linear dependence of the current densities of the anodic and cathodic peaks with the scan rate of the potential range (0; 0.8) V vs. SCE, reported during electropolymerization of 5A1N, indicates an electron transfer that is controlled by diffusion. A covalent functionalization of the RGO sheets with P5A1N is argued by: (i) the simultaneous disappearance of the IR band at 1584 cm−1 and the appearance of the new IR bands at 812, 976 and 3744 cm−1, and (ii) the appearance of two Raman lines at 738 and 1428 cm−1. An application of the RGO sheets covalently functionalized with P5A1N is demonstrated to support 1,4-phenylene diisothiocyanate (PDITC), a compound used as a cross-linking agent for various biological applications. The chemical adsorption of PDITC onto the RGO sheets covalently functionalized with P5A1N, which involves the appearance of new functional groups of the type thiourea, was proven by Raman scattering and IR spectroscopy.
Zwitterionic Polymer Brush Grafted on Polyvinylidene Difluoride Membrane Promoting Enhanced Ultrafiltration Performance with Augmented Antifouling Property
Authors: Chiao Yu-Hsuan , Chen Shu-Ting , Sivakumar Mani , Ang Micah Belle Marie Yap , Patra Tanmoy , Almodovar Jorge , Wickramasinghe S. Ranil , Hung Wei-Song , Lai Juin-Yih
Published: June 2020

Superhydrophilic zwitterions on the membrane surface have been widely exploited to improve antifouling properties. However, the problematic formation of a <20 nm zwitterionic layer on the hydrophilic surface remains a challenge in wastewater treatment. In this work, we focused on the energy consumption and time control of polymerization and improved the strong hydrophilicity of the modified polyvinylidene difluoride (PVDF) membrane. The sulfobetaine methacrylate (SBMA) monomer was treated with UV-light through polymerization on the PVDF membrane at a variable time interval of 30 to 300 s to grow a poly-SBMA (PSBMA) chain and improve the membrane hydrophilicity. We examined the physiochemical properties of as-prepared PVDF and PVDF–PSBMAx using numeric analytical tools. Then, the zwitterionic polymer with controlled performance was grafted onto the SBMA through UV-light treatment to improve its antifouling properties. The PVDF–PSBMA120s modified membrane exhibited a greater flux rate and indicated bovine serum albumin (BSA) rejection performance. PVDF–PSBMA120s and unmodified PVDF membranes were examined for their antifouling performance using up to three cycles dynamic test using BSA as foulant. The PVDF-modified PSBMA polymer improved the antifouling properties in this experiment. Overall, the resulting membrane demonstrated an enhancement in the hydrophilicity and permeability of the membrane and simultaneously augmented its antifouling properties.
The Modelling of Extrusion Processes for Polymers—A Review
Authors: Hyvärinen Marko , Jabeen Rowshni , Kärki Timo
Published: June 2020

Extrusion processes are widely used in industries that aim to produce advanced solutions for increasingly sophisticated demands in the plastic, food, and pharmaceutical sectors. Though the process has been in use since the 1930s, limited information is available on the analytical computation of extrusion. Generally, production has been carried out based on empirical experience and trial-and-error approaches. The development of industrial operations is, however, best addressed by modelling the processes involved, and the flow of polymer melts and fibers in extruders has been subjected to some previous studies. Also included an overview of design of a die as well as challenges in sheet/film production. This article systematically and critically reviews the literature related to the process design, machine design, process parameters, flow models, and flow analysis of extrusion with a focus on modelling the extrusion of composite materials.
Ethylene-Octene-Copolymer with Embedded Carbon and Organic Conductive Nanostructures for Thermoelectric Applications
Authors: Slobodian Petr , Riha Pavel , Olejnik Robert , Sedlacik Michal
Published: June 2020

Hybrid thermoelectric composites consisting of organic ethylene-octene-copolymer matrices (EOC) and embedded inorganic pristine and functionalized multiwalled carbon nanotubes, carbon nanofibers or organic polyaniline and polypyrrole particles were used to form conductive nanostructures with thermoelectric properties, which at the same time had sufficient strength, elasticity, and stability. Oxygen doping of carbon nanotubes increased the concentration of carboxyl and C–O functional groups on the nanotube surfaces and enhanced the thermoelectric power of the respective composites by up to 150%. A thermocouple assembled from EOC composites generated electric current by heat supplied with a mere short touch of the finger. A practical application of this thermocouple was provided by a self-powered vapor sensor, for operation of which an electric current in the range of microvolts sufficed, and was readily induced by (waste) heat. The heat-induced energy ensured the functioning of this novel sensor device, which converted chemical signals elicited by the presence of heptane vapors to the electrical domain through the resistance changes of the comprising EOC composites.
Kierkegaard in the Anthropocene: Hope, Philosophy, and the Climate Crisis
Authors: Guyatt Ruby
Published: June 2020

What is the role of hope in the climate crisis? What type of hope does this crisis demand? How can we sustain hope, in order to resist falling into fatalistic despair or paralyzing fear, whilst always guarding against hope giving way to happy complacency? This essay considers these urgent questions through a novel encounter between the Christian philosophy of Søren Kierkegaard, and recent eco-critical and empirical research on the affectivity of climate change mobilization. I begin by outlining the scope and aims of this essay (1st section), before introducing some affective dimensions of the climate crisis (2nd section), particularly the place of hope. Next, I examine Kierkegaard’s account of hope, and explore the extent to which it corresponds to the type of hope needed in the climate crisis (3rd section). Here, I show that Kierkegaardian hope is a therapeutic practice which subverts the eco-anxiety and sense of helplessness that can otherwise prevent individuals from engaging in positive climate action. Finally, I compare Kierkegaard’s theologically grounded hope with the hope held by climate change activists without religious faith (4th section). Participating in collective climate action anchors the individual’s hopes in a larger, collective hope, which I suggest is sustainable in ways that are partially analogous to the therapeutic functions of Kierkegaard’s Christian hope.
Alienation and Establishment
Authors: Thompson Simon
Published: June 2020

Is it ever permissible for the state to formally recognize one religion? This article focuses on just one aspect of this complex question. To be specific, a critical examination is conducted of what will be called the alienation argument against establishment, according to which this sort of connection between religion and the state is wrong to the extent that it makes some people feel alienated. Whilst this argument is often dismissed in contemporary academic analyses, considerations of this kind are frequently heard in political commentary and public debate. In this article, the three most important criticisms made of the alienation argument, which are referred to as psychologism, indeterminacy and falsity, are discussed. By arguing that it may be possible to rebut these three objections, at least to some extent, the aim of the article is to mount a partial defence of the alienation argument against religious establishment. Its conclusion is that feelings of alienation should play a significant role in making all-things-considered judgements about the justifiability of particular instances of establishment.