bluuebunny/crossref_metadata_2025 huggingface dataset:
Treatment with the Topical Antimicrobial Peptide Omiganan in Mild-to-Moderate Facial Seborrheic Dermatitis versus Ketoconazole and Placebo: Results of a Randomized Controlled Proof-of-Concept Trial
Authors: Rousel Jannik , Saghari Mahdi , Pagan Lisa , Nădăban Andreea , Gambrah Tom , Theelen Bart , de Kam Marieke L. , Haakman Jorine , van der Wall Hein E. C. , Feiss Gary L. , Niemeyer-van der Kolk Tessa , Burggraaf Jacobus , Bouwstra Joke A. , Rissmann Robert , van Doorn Martijn B. A.
Published: September 2023

Facial seborrheic dermatitis (SD) is an inflammatory skin disease characterized by erythematous and scaly lesions on the skin with high sebaceous gland activity. The yeast Malassezia is regarded as a key pathogenic driver in this disease, but increased Staphylococcus abundances and barrier dysfunction are implicated as well. Here, we evaluated the antimicrobial peptide omiganan as a treatment for SD since it has shown both antifungal and antibacterial activity. A randomized, patient- and evaluator-blinded trial was performed comparing the four-week, twice daily topical administration of omiganan 1.75%, the comparator ketoconazole 2.00%, and placebo in patients with mild-to-moderate facial SD. Safety was monitored, and efficacy was determined by clinical scoring complemented with imaging. Microbial profiling was performed, and barrier integrity was assessed by trans-epidermal water loss and ceramide lipidomics. Omiganan was safe and well tolerated but did not result in a significant clinical improvement of SD, nor did it affect other biomarkers, compared to the placebo. Ketoconazole significantly reduced the disease severity compared to the placebo, with reduced Malassezia abundances, increased microbial diversity, restored skin barrier function, and decreased short-chain ceramide Cer[NSc34]. No significant decreases in Staphylococcus abundances were observed compared to the placebo. Omiganan is well tolerated but not efficacious in the treatment of facial SD. Previously established antimicrobial and antifungal properties of omiganan could not be demonstrated. Our multimodal characterization of the response to ketoconazole has reaffirmed previous insights into its mechanism of action.
KCTD5 Forms Hetero-Oligomeric Complexes with Various Members of the KCTD Protein Family
Authors: Liao Yini , Sloan Douglas C. , Widjaja Josephine H. , Muntean Brian S.
Published: September 2023

Potassium Channel Tetramerization Domain 5 (KCTD5) regulates diverse aspects of physiology, ranging from neuronal signaling to colorectal cancer. A key feature of KCTD5 is its self-assembly into multi-subunit oligomers that seemingly enables participation in an array of protein–protein interactions. KCTD5 has recently been reported to form hetero-oligomeric complexes with two similar KCTDs (KCTD2 and KCTD17). However, it is not known if KCTD5 forms hetero-oligomeric complexes with the remaining KCTD protein family which contains over two dozen members. Here, we demonstrate that KCTD5 interacts with various KCTD proteins when assayed through co-immunoprecipitation in lysed cells. We reinforced this dataset by examining KCTD5 interactions in a live-cell bioluminescence resonance energy transfer (BRET)-based approach. Finally, we developed an IP-luminescence approach to map regions on KCTD5 required for interaction with a selection of KCTD that have established roles in neuronal signaling. We report that different regions on KCTD5 are responsible for uniquely contributing to interactions with other KCTD proteins. While our results help unravel additional interaction partners for KCTD5, they also reveal additional complexities in KCTDs’ biology. Moreover, our findings also suggest that KCTD hetero-oligomeric interactions may occur throughout the KCTD family.
Alpha-Linolenic Acid and Cardiovascular Events: A Narrative Review
Authors: Bertoni Camilla , Abodi Martina , D’Oria Veronica , Milani Gregorio P. , Agostoni Carlo , Mazzocchi Alessandra
Published: September 2023

Cardiovascular diseases (CVDs) represent the leading cause of global mortality with 1.7 million deaths a year. One of the alternative systems to drug therapy to minimize the risk of CVDs is represented by alpha-linolenic acid (ALA), an essential fatty acid of the omega-3 series, known for its cholesterol-lowering effect. The main purpose of this review is to analyze the effects of ALA and investigate the relevant omega-6/omega-3 ratio in order to maintain functionally beneficial effects. Concerning the lipid-lowering preventive effects, ALA may favorably affect the values of LDL-C and triglycerides in both adult and pediatric populations. Furthermore, ALA has shown protective effects against hypertension, contributing to balancing blood pressure through customary diet. According to the 2009 EFSA statement, dietary ALA may contribute to reducing the risk of CVDs, thanks to anti-hypertensive, anti-atherosclerotic and cardioprotective effects.
FLII Modulates the Myogenic Differentiation of Progenitor Cells via Actin Remodeling-Mediated YAP1 Regulation
Authors: Nguyen Mai Thi , Ly Quoc Kiet , Kim Hyun-Jung , Lee Wan
Published: September 2023

The dynamic rearrangement of the actin cytoskeleton plays an essential role in myogenesis, which is regulated by diverse mechanisms, such as mechanotransduction, modulation of the Hippo signaling pathway, control of cell proliferation, and the influence of morphological changes. Despite the recognized importance of actin-binding protein Flightless-1 (FLII) during actin remodeling, the role played by FLII in the differentiation of myogenic progenitor cells has not been explored. Here, we investigated the roles of FLII in the proliferation and differentiation of myoblasts. FLII was found to be enriched in C2C12 myoblasts, and its expression was stable during the early stages of differentiation but down-regulated in fully differentiated myotubes. Knockdown of FLII in C2C12 myoblasts resulted in filamentous actin (F-actin) accumulation and inhibited Yes-associated protein 1 (YAP1) phosphorylation, which triggers its nuclear translocation from the cytoplasm. Consequently, the expressions of YAP1 target genes, including PCNA, CCNB1, and CCND1, were induced, and the cell cycle and proliferation of myoblasts were promoted. Moreover, FLII knockdown significantly inhibited the expression of myogenic regulatory factors, i.e., MyoD and MyoG, thereby impairing myoblast differentiation, fusion, and myotube formation. Thus, our findings demonstrate that FLII is crucial for the differentiation of myoblasts via modulation of the F-actin/YAP1 axis and suggest that FLII is a putative novel therapeutic target for muscle wasting.
βPix Guanine Nucleotide Exchange Factor Regulates Regeneration of Injured Peripheral Axons
Authors: Jeon Yewon , Shin Yoon Kyung , Kim Hwigyeong , Choi Yun Young , Kang Minjae , Kwon Younghee , Cho Yongcheol , Chi Sung Wook , Shin Jung Eun
Published: September 2023

Axon regeneration is essential for successful recovery after peripheral nerve injury. Although growth cone reformation and axonal extension are crucial steps in axonal regeneration, the regulatory mechanisms underlying these dynamic processes are poorly understood. Here, we identify βPix (Arhgef7), the guanine nucleotide exchange factor for Rac1 GTPase, as a regulator of axonal regeneration. After sciatic nerve injury in mice, the expression levels of βPix increase significantly in nerve segments containing regenerating axons. In regrowing axons, βPix is localized in the peripheral domain of the growth cone. Using βPix neuronal isoform knockout (NIKO) mice in which the neuronal isoforms of βPix are specifically removed, we demonstrate that βPix promotes neurite outgrowth in cultured dorsal root ganglion neurons and in vivo axon regeneration after sciatic nerve crush injury. Activation of cJun and STAT3 in the cell bodies is not affected in βPix NIKO mice, supporting the local action of βPix in regenerating axons. Finally, inhibiting Src, a kinase previously identified as an activator of the βPix neuronal isoform, causes axon outgrowth defects in vitro, like those found in the βPix NIKO neurons. Altogether, these data indicate that βPix plays an important role in axonal regrowth during peripheral nerve regeneration.
Resistant and Susceptible Pinus thunbergii ParL. Show Highly Divergent Patterns of Differentially Expressed Genes during the Process of Infection by Bursaphelenchus xylophilus
Authors: Sun Tingyu , Rahman Mati Ur , Wu Xiaoqin , Ye Jianren
Published: September 2023

Pine wilt disease (PWD) is a devastating disease that threatens pine forests worldwide, and breeding resistant pines is an important management strategy used to reduce its impact. A batch of resistant seeds of P. thunbergii was introduced from Japan. Based on the resistant materials, we obtained somatic plants through somatic embryogenesis. In this study, we performed transcriptome analysis to further understand the defense response of resistant somatic plants of P. thunbergii to PWD. The results showed that, after pine wood nematode (PWN) infection, resistant P. thunbergii stimulated more differential expression genes (DEGs) and involved more regulatory pathways than did susceptible P. thunbergii. For the first time, the alpha-linolenic acid metabolism and linoleic acid metabolism were intensively observed in pines resisting PWN infection. The related genes disease resistance protein RPS2 (SUMM2) and pathogenesis-related genes (PR1), as well as reactive oxygen species (ROS)-related genes were significantly up-expressed in order to contribute to protection against PWN inoculation in P. thunbergii. In addition, the diterpenoid biosynthesis pathway was significantly enriched only in resistant P. thunbergii. These findings provided valuable genetic information for future breeding of resistant conifers, and could contribute to the development of new diagnostic tools for early screening of resistant pine seedlings based on specific PWN-tolerance-related markers.
Phylogeny of Rhynchium and Its Related Genera (Hymenoptera: Eumeninae) Based on Universal Single-Copy Orthologs and Ultraconserved Elements
Authors: Dai Min , He Shu-Lin , Chen Bin , Li Ting-Jing
Published: September 2023

The subfamily Eumeninae is a large group of fierce predatory insects that prey mainly on the larvae of Lepidoptera pests. Because of the highly similar morphologies of the genus Rhynchium and its related genera in the subfamily, including Rhynchium Spinola, Allorhynchium van der Vecht, Anterhynchium de Saussure, Pararrhynchium de Saussure, it is essential to delineate their relationships. A previous phylogenetic analysis based on mitochondrial genomes suggested the inconsistent relationships of these genera under traditional classification based on morphological characters. In this study, we first used single-copy orthologs [USCO] and ultraconserved elements [UCE] extracted from 10 newly sequenced low-coverage whole genomes to resolve the phylogenetic relationships of the above genera. The newly sequenced genomes are 152.99 Mb to 211.49 Mb in size with high completeness (BUSCO complete: 91.5–95.6%) and G + C content (36.31–38.76%). Based on extracted 5811 USCOs and 2312 UCEs, the phylogenetic relationships of Rhynchium and its related genera were: ((Allorhynchium + Lissodynerus) + (Pararrhynchium + (Anterhynchium + (Dirhynchium + Rhynchium)))), which was consistent with the mitochondrial genome results. The results supported the genus Rhynchium as monophyletic, whereas Anterhynchium was recovered as paraphyletic, with Anterhynchium (Dirhynchium) as a sister to Rhynchium and hence deserving generic status; In addition, in the genus Pararrhynchium, P. septemfasciatus feanus and P. venkataramani were separated, not clustered on a branch, just as P. septemfasciatus feanus was not together with P. striatum based on mitochondrial genomes. Since Lissodynerus septemfasciatus, the type species of the genus Lissodynerus, was transferred to Pararrhynchium, it is considered that the genus Lissodynerus should be restituted as a valid genus, not a synonym of Pararrhynchium.
The Role of Advanced Glycation End-Product Levels Measured by Skin Autofluorescence in the Development of Mitral Annular Calcification
Authors: Boyraz Bedrettin , Peker Tezcan
Published: September 2023

As a person ages, mitral annular calcification develops in the mitral annulus with increasing frequency. Lipid deposition, inflammation, and aging-related degeneration have been cited as potential causes of this pathophysiology, though there is currently no conclusive evidence to support this. AGEs accumulate in tissues due to the glycation of proteins and lipids, increasing the release of proinflammatory cytokines secondary to oxidative stress through the AGE receptor. The AGE levels increase in diabetic microvascular complications and degenerative aortic valve disease. Our study was planned prospectively as a case–control study involving 94 MAC-positive patients and 94 MAC-negative patients. The demographics, echocardiographic data and AGE levels of the patients were measured and recorded using the skin autofluorescence method. AGE levels were significantly higher in the MAC-positive patient group (3.2 vs. 2.7; p < 0.001). The AGE levels were observed as an independent predictor of MAC development in a regression analysis (OR: 8.05, 95% CI: 3.74–17.33, p < 0.001). In a ROC-curve analysis, the AUC was 0.79 (95% CI: 0.72–0.85). At a cut-off value of 2.7, 79.7% sensitivity and 69.1% specificity were observed. AGE levels can be used to cheaply, easily and non-invasively identify patients at risk of developing MAC.
Contemporary Methods for Detection and Intervention of Distal Medium and Small Vessel Occlusions
Authors: Piscopo Anthony , Zanaty Mario , Dlouhy Kathleen
Published: September 2023

The efficacy of using mechanical thrombectomy for proximal large vessel occlusions has been demonstrated in multiple large-scale trials and has further raised the question of its potential utility for distal medium and small vessel occlusions (DMSVOs). Their longer, more tortuous course and smaller corresponding vascular territories render a significant challenge for detection and intervention. The aim of this study is to provide a comprehensive overview of the current imaging and endovascular intervention options for DMSVOs and review the current works in the literature. Compared with traditional computed tomography angiography (CTA) and CT perfusion, recent advances such as multiphase CTA and maps derived from the time-to-maximum parameter coupled with artificial intelligence have demonstrated increased sensitivity for the detection of DMSVOs. Furthermore, newer generations of mini stent retrievers and thromboaspiration devices have allowed for the access and navigation of smaller and more fragile distal arteries. Preliminary studies have suggested that mechanical thrombectomy using this newer generation of devices is both safe and feasible in distal medium-sized vessels, such as M2. However, endovascular intervention utilizing such contemporary methods and devices must be balanced at the discretion of operator experience and favorable vascular anatomy. Further large-scale multicenter clinical trials are warranted to elucidate the indications for as well as to strengthen the safety and efficacy of this approach.
Comment on Lunz et al. Impact and Modification of the New PJI-TNM Classification for Periprosthetic Joint Infections. J. Clin. Med. 2023, 12, 1262
Authors: Alt Volker , Walter Nike , Rupp Markus , Baertl Susanne
Published: September 2023

We read with great interest the article by Lunz et al. [...]
Evaluation of Postoperative Analgesic Efficacy of Ultrasound-Guided Suprainguinal Fascia Iliaca Block in Knee Arthroplasty: Prospective, Randomized, Feasibility Study
Authors: Kefeli Çelik Hale , Tulgar Serkan , Güler Serkan , Koç Kadem , Küçükordulu Büşra Burcu , Ferli Ramazan Burak , Kehribar Lokman , Genç Ahmet Serhat , Süren Mustafa
Published: September 2023

Background: Total Knee Arthroplasty (TKA) is one of the most commonly performed orthopedic procedures, and patients complain of severe pain in the postoperative period. The supra-inguinal fascia iliaca block (SIFIB) works as an anteriorly applied lumbar plexus block and is frequently used in hip surgeries. In this study, we evaluated the effect of SIFIB in patients undergoing TKA under spinal anesthesia. Methods: This study is a prospective, randomized, assessor-blinded feasibility study conducted in a tertiary hospital. Eighty-six patients with ASA I-III were initially enrolled, and after exclusions, 80 patients were randomized into two equal groups (SIFIB and control groups). The standard multimodal analgesia was applied to the control group, while SIFIB was additionally applied to the block group. The study measured the morphine requirement in PCA and pain intensity using Numeric Rating Scores between the two groups. Results: the 24-h cumulative morphine consumption was lower in Group SIFIB. Although there was a decrease in NRS at rest scores in the SIFIB group during some time periods, pain was moderate, and no differences in pain scores were recorded during exercise in all patients. Conclusions: In patients undergoing TKA under spinal anesthesia, a single shot of SIFIB results in a significant reduction in the amount of morphine consumed in hours. This effect was most likely related to a decrease in pain at rest in the SIFIF group.
The Use of Capacitive and Resistive Energy Transfer in Postpartum Pain Management in Women after Perineal Trauma
Authors: Siereńska Joanna , Sotomska Zofia , Madej-Łukasiak Dorota , Wąż Piotr , Grzybowska Magdalena Emilia
Published: September 2023

Perineal pain occurs in 97% of women with episiotomy or first- and second-degree perineal tears on the first day after delivery. The study aimed to assess the impact of capacitive and resistive energy transfer (TECAR) on perineal pain and discomfort in the first two postpartum days. The prospective randomized double-blind study was performed with the pain and discomfort assessment using the Visual Analogue Scale at baseline and after both TECAR interventions. Characteristics data, delivery information, and the number of painkillers taken were collected. The assumed significance level was α < 0.05. The study included 121 women with a mean age of 30.7 ± 4.2 years and a median BMI of 26.1 kg/m2 (24.1; 28.9). Pain reduction at rest, when walking, and discomfort reduction when walking were significantly higher in the TECAR group compared to the sham group (p < 0.05). After the first TECAR intervention, significant reduction in all measured parameters was observed in the study group (p < 0.03), whereas in the control group, it was observed in pain and discomfort while sitting (p < 0.04). The amount of ibuprofen taken on the second day was significantly reduced in the study group compared to the first day (p = 0.004). TECAR has been shown to provide more immediate and significant reduction in perineal pain and discomfort.
The Worsening of Heart Failure with Reduced Ejection Fraction: The Impact of the Number of Hospital Admissions in a Cohort of Patients
Authors: Perea-Armijo Jorge , López-Aguilera José , González-Manzanares Rafael , Pericet-Rodriguez Cristina , Castillo-Domínguez Juan Carlos , Heredia-Campos Gloria , Roldán-Guerra Álvaro , Urbano-Sánchez Cristina , Barreiro-Mesa Lucas , Aguayo-Caño Nerea , Delgado-Ortega Mónica , Crespín-Crespín Manuel , Ruiz-Ortiz Martín , Mesa-Rubio Dolores , Osorio Manuel Pan-Álvarez , Anguita-Sánchez Manuel
Published: September 2023

Background: Worsening heart failure (WFH) includes heart failure (HF) hospitalisation, representing a strong predictor of mortality in patients with heart failure with reduced ejection fraction (HFrEF). However, there is little evidence analysing the impact of the number of previous HF admissions. Our main objective was to analyse the clinical profile according to the number of previous admissions for HF and its prognostic impact in the medium and long term. Methods: A retrospective study of a cohort of patients with HFrEF, classified according to previous admissions: cohort-1 (0–1 previous admission) and cohort-2 (≥2 previous admissions). Clinical, echocardiographic and therapeutic variables were analysed, and the medium- and long-term impacts in terms of hospital readmissions and cardiovascular mortality were assessed. A total of 406 patients were analysed. Results: The mean age was 67.3 ± 12.6 years, with male predominance (73.9%). Some 88.9% (361 patients) were included in cohort-1, and 45 patients (11.1%) were included in cohort-2. Cohort-2 had a higher proportion of atrial fibrillation (49.9% vs. 73.3%; p = 0.003), chronic kidney disease (36.3% vs. 82.2%; p < 0.001), and anaemia (28.8% vs. 53.3%; p = 0.001). Despite having similar baseline ventricular structural parameters, cohort-1 showed better reverse remodelling. With a median follow-up of 60 months, cohort-1 had longer survival free of hospital readmissions for HF (37.5% vs. 92%; p < 0.001) and cardiovascular mortality (26.2% vs. 71.9%; p < 0.001), with differences from the first month. Conclusions: Patients with HFrEF and ≥2 previous admissions for HF have a higher proportion of comorbidities. These patients are associated with worse reverse remodelling and worse medium- and long-term prognoses from the early stages, wherein early identification is essential for close follow-up and optimal intensive treatment.
Real-Time Neuropsychological Testing (RTNT) and Music Listening during Glioblastoma Excision in Awake Surgery: A Case Report
Authors: D’Onofrio Grazia , Icolaro Nadia , Fazzari Elena , Catapano Domenico , Curcio Antonello , Izzi Antonio , Manuali Aldo , Bisceglia Giuliano , Tancredi Angelo , Marchello Vincenzo , Recchia Andreaserena , Tonti Maria Pia , Pazienza Luca , Carotenuto Vincenzo , Bonis Costanzo De , Savarese Luciano , Gaudio Alfredo Del , Gorgoglione Leonardo Pio
Published: September 2023

In this case report, real-time neuropsychological testing (RTNT) and music listening were applied for resections in the left temporal–parietal lobe during awake surgery (AS). The case is based on a 66-year-old with glioblastoma and alterations in expressive language and memory deficit. Neuropsychological assessment was run at baseline (2–3 days before surgery), discharge from hospital (2–3 days after surgery), and follow-up (1 month and 3 months). RTNT was started before beginning the anesthetic approach (T0) and during tumor excision (T1 and T2). At T0, T1, and T2 (before performing neuropsychological tests), music listening was applied. Before AS and after music listening, the patient reported a decrease in depression and anxiety. During AS, an improvement was shown in all cognitive parameters collected at T0, T1, and T2. After the excision and music listening, the patient reported a further decrease in depression and anxiety. Three days post surgery, and at follow-ups of one month and three months, the patient reported a further improvement in cognitive aspects, the absence of depression, and a reduction in anxiety symptoms. In conclusion, RTNT has been useful in detecting cognitive function levels during tumor excision. Music listening during AS decreased the patient’s anxiety and depression symptoms.
The “Woggle” Technique for Venous Access Site Management: An Old Technique for a New Need
Authors: Tumminello Gabriele , Barbieri Lucia , Avallone Carlo , Bellissimo Nello , Mircoli Luca , Colombo Federico , Vicenzi Marco , Ruscica Massimiliano , Carugo Stefano
Published: September 2023

Background: Several closure devices are routinely used for percutaneous arterial access, while a relatively low number is available for the management of large bore venous accesses. The Woggle technique is a modification of the purse-string suture which was introduced several years ago in patients undergoing hemodialysis. Methods: A population of 45 patients who underwent transvenous femoral structural heart interventions was retrospectively evaluated. The Woggle technique consists of a purge string suture with a collar to maintain the tension as stable over time and a suture lock to tighten the suture. Results: Sheaths magnitude ranged from 8 French (F) to 14 F. A rapid post-procedural hemostasis was achieved in the whole population, and in 95% of cases, definite hemostasis was obtained after the first single release; the mean time of release was 302 ± 83 min. Although no relevant bleedings were reported, a significant reduction in hemoglobin levels was found in the whole population. This decrement was statistically significant only in the group with sheaths higher than 12 F. A single mild local hematoma was recorded in the group in which smaller sheaths were used. Seventy-two percent of patients were pre-treated with a dual antiplatelet therapy. Conclusions: The Woggle technique has shown to be a simple, effective, and safe approach for the management of large bore venous in percutaneous structural heart interventions.
Cardiovascular Safety Evaluation of Febuxostat and Allopurinol: Findings from the FDA Adverse Event Reporting System
Authors: Bai Yang , Wu Bin , Gou Liangwen , Fang Zhenwei , Xu Ting , Zhang Tiejun , Li Yuwen
Published: September 2023

Background: Febuxostat and allopurinol are the most commonly used uric acid-lowering medications, and their safety is of great concern, especially the cardiovascular adverse reactions associated with febuxostat. We propose to study the cardiovascular toxicity of febuxostat and allopurinol using the FDA Adverse Event Reporting System (FAERS) database. Methods: A total of 64 quarters of FAERS data were downloaded from 2004 to 2019. Febuxostat- and allopurinol-related cardiovascular adverse events were extracted after data cleaning. Signal detection was conducted by reporting odds ratio (ROR) and proportional reporting ratio (PRR). Results: There were 2939 and 25,219 reports of febuxostat- and allopurinol-related cardiovascular adverse events (CVAEs), respectively. The most frequent CVAEs with febuxostat and allopurinol were edema peripheral (14.38%) and peripheral swelling (8.76%), respectively. In elderly gout patients, febuxostat is associated with an increased risk of heart failure, ischemic heart disease, hypertension, and cardiomyopathy. Febuxostat in combination with acetic acid derivatives nonsteroidal anti-inflammatory drug (NSAIDS) also increases the risk of cardiovascular adverse events. Conclusions: Compared with allopurinol, febuxostat may increase cardiovascular toxicity in patients with gout.
Retroperitoneal Single-Port Robot-Assisted Nephroureterectomy with Bladder Cuff Excision: Initial Experience and Description of the Technique
Authors: Bang Seokhwan , Cho Hyuk Jin , Ha U-Syn , Lee Ji Youl , Hong Sung-Hoo
Published: September 2023

Background: With the introduction of the single-port (SP) robot, surgery that was difficult to attempt is becoming possible. Nephroureterectomy (NUx) for upper tract urothelial carcinoma also seems to be able to attempt a retroperitoneal (RP) approach. Purpose: to investigate the feasibility of SP robotic RP NUx with bladder cuff excision. Design, setting, and participants: we sequentially analyzed 20 patients who underwent SP robot NUx from January 2021 to December 2022. Surgical procedure: all patients were diagnosed with upper tract urothelial carcinoma (UTUC) and were operated upon by a single expert using the da Vinci SP platform (Intuitive Surgical, Sunnyvale, CA, USA) with retroperitoneal approach. Results and limitations: A total of 20 patients underwent SP robotic NUx with bladder cuff excision. The mean age of patients was 69.45 ± 8.68 years, and the mean body mass index (BMI) was 25.37 ± 3.00 kg/m2. The mean tumor size was 2.42 ± 1.03 cm on a CT scan, with right-sided tumors in eight patients (40%) and left-sided tumors in 12 patients (60%). The median console time was 106 min and 40 s, and the expected blood loss was 122.50 ± 75.18 mL. Final pathology showed that all of the patients were diagnosed as having urothelial carcinoma; one patient was classified as Ta (5.00%), three patients were classified as T1 (15.00%), seven patients were classified as T2 (35.00%), eight patients were classified as T3 (40.00%), and one patient was classified as T4 (5.00%). None of these 20 patients showed any complications based on the Clavien–Dindo scale. Conclusions: SP robotic NUx using a retroperitoneal approach provides feasible perioperative and postoperative outcomes for UTUC.
Subfertility as Overlapping of Nutritional, Endocrine, Immune, and Cardiometabolic Dysregulations—A Study Focused on Biochemical Endophenotypes of Subfertile Couples
Authors: Wasilewski Tadeusz , Wasilewska Jolanta , Łukaszewicz-Zając Marta , Mroczko Barbara
Published: September 2023

Subfertility is a global health issue, and as many as 30% of cases are attributed to unexplained reasons. A hypercaloric, high-fat diet stimulates the expansion of pro-inflammatory gut microbiota with a consequent rise in circulating lipopolysaccharides. Adverse gut microbiota remodeling can exacerbate insulin resistance, while sex and thyroid hormones may influence the variability in gut microbiota. This cross-sectional study included 150 participants and was designed to determine a biochemical, nutritional-related pattern that may distinguish subfertile from fertile individuals and couples. A panel of 28 biomarkers was assessed. Four biochemical phenotypes of unexplained subfertility were found, including two metabolic and two immune, when assessed using binary logistic regression models. Two phenotypes were distinguished in women: cardio-metabolic with atherogenic dyslipidemia (LowHDL-cholesterol: OR = 10.9; p < 0.05) and autoimmune thyroid disorder (Highanti-thyroid-peroxidase: OR = 5.5; p < 0.05) and two in men: hepato-metabolic with elevated liver injury enzymes (HighHOMA-IR: OR = 6.1; p < 0.05) and immune type-2 response (HighIgE: OR = 6.4; p < 0.05). The chances of a couple’s subfertility rose with the number of laboratory components of metabolic syndrome in the couple (OR = 1.7; p < 0.05) and if at least one partner had an elevated total IgE level (>100 kU/L) (OR = 6.5; p < 0.05). This study found that unexplained subfertility may be accompanied by mutually overlapping immune and metabolic dysregulations in individuals and couples. We propose one-time laboratory diagnostics taking into account the lipid profile, insulin resistance, anti-thyroid-peroxidase, and total IgE in both males and females with unexplained subfertility. This may allow for a one-time assessment of targeted medical and nutritional interventions and help optimize patients’ health. The gut–organ axes related to subfertility are discussed in the context of the obtained results.
The Impact of Coastline and Bathymetry Changes on the Storm Tides in Zhejiang Coasts
Authors: Gou Xiaoxiao , Liang Huidi , Cai Tinglu , Wang Xinkai , Chen Yining , Xia Xiaoming
Published: September 2023

Coastal evolutions are expected to have a significant impact on storm tides, disproportionately aggravating coastal flooding. In this study, we utilize a nested storm tide model to provide an integrated investigation of storm tide responses to changes in coastline and bathymetry along the Zhejiang coasts. We selected coastline and bathymetry data from 1980 and 2016, as well as data from three typical typhoon events (i.e., Winnie, Haikui, and Chan-hom) for simulating the storm surge processes. The results indicate that changes in the coastline and bathymetry from 1980 to 2016 have resulted in an increase in storm tides in the northern part and a decrease in the central part of Zhejiang. Specifically, storm tides in Hangzhou Bay have increased significantly, with an average increase of about 0.3 m in the maximum storm tides primarily attributed to coastline changes. On the contrary, in smaller basins like Sanmen Bay, while reclamation itself has reduced peak storm surges, rapid siltation has consequently exacerbated the storm surge. By decomposing storm tides into astronomical tides and storm surges, we discovered that the change in tidal levels was twice as significant as the surge change. Moreover, the nonlinear tide–surge interaction was nearly four times that of the pure surge, significantly contributing to storm surge variation. Alterations in the momentum balance reveal that the water depth-induced bottom friction and wind stress increase contributes to the local enlargement of storm tides at the bay head, while the coastline changes exaggerate nearshore storm tides through an increase in the advection term.
When Time Is of the Essence—Recording an Underwater Excavation at 110 m
Authors: Gambin Timmy , Sausmekat Maja , Wood John , Hyttinen Kari
Published: September 2023

Underwater archaeological excavations present unique challenges, particularly when conducted at significant depths where divers face limited time on the archaeological site. Traditional methods of recording excavation progress, such as manual documentation and drawings, are too time-consuming when the time on the site totals no more than 12 min. To address these limitations, this paper introduces an innovative approach for daily recording and observation of underwater archaeological excavations using 3D photogrammetric models and point of view (PoV) cameras developed specifically for the excavation of a shipwreck situated at a depth of 110 m. The proposed system leverages advancements in photogrammetry and 3D modelling techniques to capture and analyse detailed visual data of the excavation site on a daily basis. Three-dimensional photogrammetric models offer a comprehensive and easily accessible representation of the excavation site, enabling the team to record and analyse excavation levels and features in a highly precise and detailed manner. By comparing the 3D models captured on consecutive days, researchers can precisely measure changes in the excavation area, identify new artifacts or structures, and record the progress of the excavation. The PoV cameras contribute to the planning of daily tasks required on the site. Overall, this paper showcases innovative methods for underwater archaeological excavations at significant depths, employing 3D photogrammetric models for daily recording as well as PoV cameras for observation. The proposed system represents a substantial advancement in the field of underwater archaeology, offering a more accurate, efficient, and reliable approach to documenting and analysing excavation progress, whilst also producing results that can be subsequently adapted and applied to other deep water archaeological sites.
The Influence of Geological Conditions in the Hangzhou Bay Area on the Deformation Behavior of Deep Excavations
Authors: Zhu Yihong , Wu Mingming , Zhang Gangping , Yu Jianlin , Xu Qiliang , Xu Riqing , Yu Tianci
Published: September 2023

The deformation behavior of deep excavations is affected by many factors, among which the geological conditions are greatly affected. Hangzhou Bay is affected by marine siltation and river alluvium, and the geological conditions within the urban area of Hangzhou are quite different. In this paper, the geological and deformation data of 79 deep excavation cases in the Hangzhou urban area were collected, and the statistical analysis showed that the deformation control of excavations in the silt area was poor. The average maximum lateral wall displacement of deep excavations of the Hangzhou urban area was 0.41%H (H was the depth of the excavation), the average value of the alluvial area was 0.22%H, and the average value of the silted area was 0.55%H. The influence of geological conditions, wall type, and construction period on the deformation of excavations was compared, and the deformation behavior of excavations in the silted area was clearly affected by various factors.
Indigo Carmine: Between Necessity and Concern
Authors: Ristea Madalina-Elena , Zarnescu Otilia
Published: September 2023

Dyes, such as indigo carmine, have become indispensable to modern life, being widely used in the food, textile, pharmaceutical, medicine, and cosmetic industry. Although indigo carmine is considered toxic and has many adverse effects, it is found in many foods, and the maximum permitted level is 500 mg/kg. Indigo carmine is one of the most used dyes in the textile industry, especially for dyeing denim, and it is also used in medicine due to its impressive applicability in diagnostic methods and surgical procedures, such as in gynecological and urological surgeries and microsurgery. It is reported that indigo carmine is toxic for humans and can cause various pathologies, such as hypertension, hypotension, skin irritations, or gastrointestinal disorders. In this review, we discuss the structure and properties of indigo carmine; its use in various industries and medicine; the adverse effects of its ingestion, injection, or skin contact; the effects on environmental pollution; and its toxicity testing. For this review, 147 studies were considered relevant. Most of the cited articles were those about environmental pollution with indigo carmine (51), uses of indigo carmine in medicine (45), and indigo carmine as a food additive (17).
Factors Affecting the Potential Efficacy of Intrauterine Platelet-Rich Plasma Infusion on Thin Endometrium in Women with Recurrent Implantation Failure
Authors: Lin Pin-Yao , Lee Chun-I , Chen Yi-Chun , Cheng En-Hui , Huang Chun-Chia , Chen Chung-I , Lee Tsung-Hsien , Lee Yu-Jen , Lee Maw-Sheng
Published: September 2023

Optimizing endometrial thickness (EMT) is crucial for successful embryo implantation, but enhancing thin endometrium remains a significant challenge. Platelet-rich plasma (PRP)-derived therapies have emerged as a promising approach in reproductive medicine due to their capacity to facilitate tissue repair and regeneration. This study aims to identify the risk factors associated with the failure of intrauterine PRP infusion for thin endometrium in women with recurrent implantation failure (RIF). We retrospectively reviewed data from 77 women with RIF, all exhibiting an EMT of <7 mm. These women underwent programmed hormone therapy for frozen embryo transfer (FET) and received two autologous intrauterine PRP infusions. Following intrauterine PRP-lysate (PL) infusions, the mean increase in EMT was 1.9 ± 1.2 mm, with EMT reaching 7 mm in 86% of the cases (66/77; average EMT, 8.3 mm). We identified an exceedingly thin EMT as a risk factor impacting the therapeutic efficacy in increasing EMT (p = 0.04, OR: 3.16; 95% CI: 1.03–9.67). Additionally, the number of previous uterine surgeries emerged as a prognostic factor for pregnancy failure following PL infusion (p = 0.02, OR: 2.02; 95% CI: 1.12–3.64). Our findings suggest that an extremely thin EMT and a history of numerous uterine surgeries can impede successful pregnancy, even when an optimal EMT is achieved following PRP infusion.
AI-Driven Decision Support for Early Detection of Cardiac Events: Unveiling Patterns and Predicting Myocardial Ischemia
Authors: Elvas Luís B. , Nunes Miguel , Ferreira Joao C. , Dias Miguel Sales , Rosário Luís Brás
Published: September 2023

Cardiovascular diseases (CVDs) account for a significant portion of global mortality, emphasizing the need for effective strategies. This study focuses on myocardial infarction, pulmonary thromboembolism, and aortic stenosis, aiming to empower medical practitioners with tools for informed decision making and timely interventions. Drawing from data at Hospital Santa Maria, our approach combines exploratory data analysis (EDA) and predictive machine learning (ML) models, guided by the Cross-Industry Standard Process for Data Mining (CRISP-DM) methodology. EDA reveals intricate patterns and relationships specific to cardiovascular diseases. ML models achieve accuracies above 80%, providing a 13 min window to predict myocardial ischemia incidents and intervene proactively. This paper presents a Proof of Concept for real-time data and predictive capabilities in enhancing medical strategies.
Distribution and Management of the Invasive Swietenia macrophylla King (Meliaceae) at the Foot of a Protected Area in Luzon Island, Philippines
Authors: Coracero Ericson Esquibel
Published: September 2023

Invasive alien plant species (IAPS) pose one of the most significant threats to native biodiversity. Swietenia macrophylla, or big leaf mahogany, is among the most threatening invasive plants in the Philippines. This article aimed to formally document the presence of S. macrophylla along the edges of Mt. Banahaw de Nagcarlan, a protected area on Luzon Island, Philippines. The study also sought to identify the management strategies being implemented by various government institutions to address big leaf mahogany and other invasive plants. A total of 1591 individuals of S. macrophylla were documented in mixed land-use areas and roadsides. These were found to have been introduced by the Department of Environment and Natural Resources in 1991 as a reforestation species. Fortunately, no individuals were observed beyond the buffer zone towards the protected area. The identification of management strategies for big leaf mahogany and other IAPS revealed that there is no established approach specifically addressing the presence of S. macrophylla at the site. However, some institutions advocate for the conservation of native plants through tree planting activities and educational campaigns. Furthermore, no collaborative efforts were observed among stakeholders and institutions. The results of this study highlight the urgent need to manage the S. macrophylla population. Planning and enforcement of strategies require collaborative efforts among stakeholders to prevent its entry into the protected area and ensure the preservation of native biodiversity.
Assessing the Influence of Water Conservancy Projects on China’s Reserve Resources for Cultivated Land
Authors: Yao Yuan , He Guohua , Li Wei , Zhao Yong , Li Haihong , He Fan
Published: September 2023

The development of reserve resources of cultivated land (RRCL) is a vital way of supplementing cultivated land in the northern arid and semi-arid regions of China. This study developed a suitability evaluation system for reserve resources of cultivated land from the nature–function–environment perspective. The evaluation considered the construction of water conservancy projects, establishing two scenarios of rain-fed and irrigated agriculture. The evaluation results for unused land were divided into four levels, including highly suitable, relatively suitable, moderately suitable and unsuitable, using the three-dimensional Rubik’s cube spatial classification method. The results show that: (1) Under the rain-fed agriculture scenario, the developable quantity and quality of unused land were poor. The potential area of reserve resources of cultivated land only accounted for 5% of the total area. (2) Under the irrigated agriculture scenario, the potential area of reserve resources of cultivated land increased to 21% of the total unused land. The areas were mainly distributed in Xinjiang, Inner Mongolia, and Gansu. The research considered the impact of water conservancy projects on the development and utilization of reserve resources of cultivated land. It can provide references for the development of arable land resources and territorial spatial planning in China.
Quantitative Evaluation of China’s Central-Level Land Consolidation Policies in the Past Forty Years Based on the Text Analysis and PMC-Index Model
Authors: Huang Guodong , Shen Xiaoqiang , Zhang Xiaobin , Gu Wei
Published: September 2023

After nearly forty years of development, China’s land consolidation policies (CLCP) have become an important tool for promoting rural revitalization and sustainable development. However, as a major land management policy, there is still a lack of quantitative evaluation research on its text. This paper establishes an evaluation system for CLCP using text analysis and the PMC-Index model. Based on a reasonable definition of the connotation of land consolidation, this paper collects 313 related policies issued by China’s central government from 1982 to 2022, using text analysis to sort out the characteristics of issuance time, policy types, issuing institutions, and cooperation networks. By combining the outcomes of text mining with the previous research results to set evaluation indicators for the PMC-Index model, it distinguishes between comprehensive policies and specialized policies and separately evaluates them. Then, the PMC-Surface is established to clearly display the calculation results. The results show that the evaluation scores of comprehensive policies and specialized policies showed an upward trend over time. This indicates that the content of CLCP is constantly being enriched and expanded. The evaluation scores for different dimensions of comprehensive policies are relatively balanced, whereas there are significant differences in the evaluation scores of various dimensions of specialized policies. Both comprehensive policies and specialized policies have weaknesses in policy functions, incentives and constraints, and implementation guarantees, so improvements may be needed in these areas in the future. This study provides valuable insights into the advantages and disadvantages of a single land consolidation policy in China.
An Investigation of the High-Speed Machinability of 7050 Aluminum Alloy Based on Different Prefabricated Crystal Orientations
Authors: Ni Chenbing , Lu Wei , Wang Youqiang , Zong Chengguo , Liu Dejian , Liu Guoliang
Published: September 2023

This study investigated the high-speed cutting performance of 7050 aluminum alloy with prefabricated crystal orientations under dry-cutting conditions. Three specimens with different crystal orientations were prefabricated using pre-deformations of 10, 15, and 20%, and the effects of cutting parameters on cutting force, surface morphology, and tool wear were analyzed. The results showed that the three-dimensional cutting force initially increased and then decreased with the increase in cutting speed. In addition, the three-dimensional cutting force increased with the increase in cutting depth and feed rate. Under the same cutting parameters, the three-dimensional cutting force of 7050 aluminum alloy was in the following order: 20% pre-deformation > 10% pre-deformation > 15% pre-deformation. During high-speed cutting, different degrees of plowing, bulging, and sticky chips appeared on the machined surface, and the surface morphology of the 15% pre-deformed 7050 aluminum alloy was better than that of the other two pre-deformed 7050 aluminum alloys. During the high-speed cutting process, tool wear mainly occurred in the forms of collapse edge, adhesion, flaking, and breakage, and wear mechanisms were usually adhesive, diffusion, and oxidation wears. Under the same cutting parameters, the tool wear of the 15% pre-deformed 7050 aluminum alloy was lighter.
Hierarchical Multiple Precursors Induced Heterogeneous Structures in Super Austenitic Stainless Steels by Cryogenic Rolling and Annealing
Authors: Tan Duo , Fu Bin , Guan Wei , Li Yu , Guo Yanhui , Wei Liqun , Ding Yi
Published: September 2023

Multiple deformed substructures including dislocation cells, nanotwins (NTs) and martensite were introduced in super austenitic stainless steels (SASSs) by cryogenic rolling (Cryo-R, 77 K/22.1 mJ·m−2). With the reduction increasing, a low stacking fault energy (SFE) and increased flow stress led to the activation of secondary slip and the occurrence of NTs and martensite nano-laths, while only dislocation tangles were observed under a heavy reduction by cold-rolling (Cold-R, 293 K/49.2 mJ·m−2). The multiple precursors not only possess variable deformation stored energy, but also experience competition between recrystallization and reverse transformation during subsequent annealing, thus contributing to the formation of a heterogeneous structure (HS). The HS, which consists of bimodal-grained austenite and retained martensite simultaneously, showed a higher yield strength (~1032 MPa) and a larger tensile elongation (~9.1%) than the annealed coarse-grained Cold-R sample. The superior strength–ductility and strain hardening originate from the synergistic effects of grain refinement, dislocation and hetero-deformation-induced hardening.