bluuebunny/crossref_metadata_2025 huggingface dataset:
Collective Effervescence, Self-Transcendence, and Gender Differences in Social Well-Being During 8 March Demonstrations
Authors: Zumeta Larraitz N. , Castro-Abril Pablo , Méndez Lander , Pizarro José J. , Włodarczyk Anna , Basabe Nekane , Navarro-Carrillo Ginés , Padoan-De Luca Sonia , da Costa Silvia , Alonso-Arbiol Itziar , Torres-Gómez Bárbara , Cakal Huseyin , Delfino Gisela , Techio Elza M. , Alzugaray Carolina , Bilbao Marian , Villagrán Loreto , López-López Wilson , Ruiz-Pérez José Ignacio , Cedeño Cynthia C. , Reyes-Valenzuela Carlos , Alfaro-Beracoechea Laura , Contreras-Ibáñez Carlos , Ibarra Manuel Leonardo , Reyes-Sosa Hiram , Cueto Rosa María , Carvalho Catarina L. , Pinto Isabel R.
Published: December 2020

8 March (8M), now known as International Women’s Day, is a day for feminist claims where demonstrations are organized in over 150 countries, with the participation of millions of women all around the world. These demonstrations can be viewed as collective rituals and thus focus attention on the processes that facilitate different psychosocial effects. This work aims to explore the mechanisms (i.e., behavioral and attentional synchrony, perceived emotional synchrony, and positive and transcendent emotions) involved in participation in the demonstrations of 8 March 2020, collective and ritualized feminist actions, and their correlates associated with personal well-being (i.e., affective well-being and beliefs of personal growth) and collective well-being (i.e., social integration variables: situated identity, solidarity and fusion), collective efficacy and collective growth, and behavioral intention to support the fight for women’s rights. To this end, a cross-cultural study was conducted with the participation of 2,854 people (age 18–79; M = 30.55; SD = 11.66) from countries in Latin America (Mexico, Chile, Argentina, Brazil, Peru, Colombia, and Ecuador) and Europe (Spain and Portugal), with a retrospective correlational cross-sectional design and a convenience sample. Participants were divided between demonstration participants (n = 1,271; 94.0% female) and non-demonstrators or followers who monitored participants through the media and social networks (n = 1,583; 75.87% female). Compared with non-demonstrators and with males, female and non-binary gender respondents had greater scores in mechanisms and criterion variables. Further random-effects model meta-analyses revealed that the perceived emotional synchrony was consistently associated with more proximal mechanisms, as well as with criterion variables. Finally, sequential moderation analyses showed that proposed mechanisms successfully mediated the effects of participation on every criterion variable. These results indicate that participation in 8M marches and demonstrations can be analyzed through the literature on collective rituals. As such, collective participation implies positive outcomes both individually and collectively, which are further reinforced through key psychological mechanisms, in line with a Durkheimian approach to collective rituals.
Aesthetic Evaluation of Digitally Reproduced Art Images
Authors: Reymond Claire , Pelowski Matthew , Opwis Klaus , Takala Tapio , Mekler Elisa D.
Published: December 2020

Most people encounter art images as digital reproductions on a computer screen instead of as originals in a museum or gallery. With the development of digital technologies, high-resolution artworks can be accessed anywhere and anytime by a large number of viewers. Since these digital images depict the same content and are attributed to the same artist as the original, it is often implicitly assumed that their aesthetic evaluation will be similar. When it comes to the digital reproductions of art, however, it is also obvious that reproductions do differ from the originals in various aspects. Besides image quality, resolution, and format, the most obvious change is in the representation of color. The effects of subjectively varying surface-level image features on art evaluation have not been clearly assessed. To address this gap, we compare the evaluation of digital reproductions of 16 expressionist and impressionist paintings manipulated to have a high color saturation vs. a saturation similar to the original. We also investigate the impact of viewing time (100 ms vs. unrestricted viewing time) and expertise (art experts vs. laypersons), two other aspects that may impact the perception of art in online contexts. Moreover, we link these dimensions to a recent model of aesthetic experience [the Vienna Integrated Model of Top-Down and Bottom-Up Processes in Art Perception (VIMAP)]. Results suggest that color saturation does not exert a major influence on liking. Cognitive and emotional aspects (interest, confusion, surprise, and boredom), however, are affected – to different extents for experts and laypersons. For laypersons, the increase in color saturation led to more positive assessments of an artwork, whereas it resulted in increased confusion for art experts. This insight is particularly important when it comes to reproducing artworks digitally. Depending on the intended use, increasing or decreasing the color saturation of the digitally reproduced image might be most appropriate. We conclude with a discussion of these findings and address the question of why empirical aesthetics requires more precise dimensions to better understand the subtle processes that take place in the perception of today’s digitally reproduced art environment.
Burnout Syndrome Among Hospital Healthcare Workers During the COVID-19 Pandemic and Civil War: A Cross-Sectional Study
Authors: Elhadi Muhammed , Msherghi Ahmed , Elgzairi Moutaz , Alhashimi Ayiman , Bouhuwaish Ahmad , Biala Marwa , Abuelmeda Seraj , Khel Samer , Khaled Ala , Alsoufi Ahmed , Elmabrouk Amna , Alshiteewi Fatimah Bin , Hamed Tasneem Ben , Alhadi Bushray , Alhaddad Sara , Elhadi Ahmed , Zaid Ahmed
Published: December 2020

Objective: We aimed to determine the prevalence of burnout among hospital healthcare workers in Libya during the coronavirus disease (COVID-19) pandemic and civil war.

Methods: A cross-sectional study was conducted from April 18 to May 2, 2020 among Libyan healthcare workers. Data on participant characteristics were collected with a specifically designed questionnaire. Burnout was assessed with the abbreviated Maslach Burnout Inventory (aMBI) comprising three subscales: emotional exhaustion (EE), depersonalization (DP), and personal accomplishment (PA), with each sub-scale score range from 0 to 18. For EE and DP, scores of 10 to 18 were regarded as “moderate to severe burnout.” PA was scored inversely, where a score ≤ 10 indicated severe burnout.

Results: The study yielded a sample size of 532 participants. Of these, 357 (67.1%) reported emotional exhaustion (EE Score ≥ 10), 252 (47.4%) reported depersonalization (DP score ≥ 10), and 121 (22.7%) reported a lower sense of personal accomplishment (PA score ≤ 10). Verbal abuse was experienced by 304 participants (57.1%) and physical abuse in 93 (17.5). Gender was associated with high emotional exhaustion and high depersonalization. Being 35 years or older was associated with high depersonalization. Professional specialty was significantly associated with high emotional exhaustion and depersonalization. Fear of COVID-19 infection was associated with high emotional exhaustion and high depersonalization.

Conclusion: The rising prevalence of mental disorders and inadequate availability of health services facilities during the COVID-19 pandemic and civil war demonstrated the need for healthcare policies to address the well-being of healthcare workers to decrease the risk of loss, suicide, and medical negligence.
Anxiety Level of University Students During COVID-19 in Saudi Arabia
Authors: Khoshaim Heba Bakr , Al-Sukayt Areej , Chinna Karuthan , Nurunnabi Mohammad , Sundarasen Sheela , Kamaludin Kamilah , Baloch Gul Mohammad , Hossain Syed Far Abid
Published: December 2020

COVID-19 is the worst pandemic of this millennium, and it is considered to be the “public enemy number one.” This catastrophe has changed the way we live in the blink of an eye. Not only has it threatened our existence and health status, but the damage associated with it could equally affect our economic, social, and educational systems. The focus of this study was on the anxiety level of university students during the COVID-19 pandemic in Saudi Arabia. The study was conducted between March and June 2020. A questionnaire was administered online, and 400 completed questionnaires were returned. In this study, the Zung self-rating anxiety scale was used to determine the anxiety levels among the respondents. The results indicated that about 35% of the students experienced moderate to extreme levels of anxiety. Anxiety was highly associated with age, sex, and level of education. These findings can enlighten government agencies and policy makers on the importance of making prompt, effective decisions to address students' anxiety during the COVID-19 pandemic. Researchers are encouraged to focus their future studies on how to develop strategies to boost students' resilience and enhance their adaptability skills for similar disasters in the future.
Variation in COVID-19 Diagnosis by Zip Code and Race and Ethnicity in Indiana
Authors: Hanson Amy E. , Hains David S. , Schwaderer Andrew L. , Starr Michelle C.
Published: December 2020

Objectives: To describe variations in coronavirus disease 2019 (COVID-19) diagnosis by zip code race and ethnicity in Indiana.

Methods: Cross-sectional evaluation of subjects with SARS-CoV-2 at Indiana University Health. We performed two separate analyses, first evaluating likelihood of COVID-19 diagnosis by race (Caucasian, African American, Asian, or other) and ethnicity (Hispanic vs. non-Hispanic) in the cohort encompassing the entire state of Indiana. Subsequently, patient data was geolocated with zip codes in Marion County and the immediate surrounding counties, and descriptive statistical analyses were used to calculate the number of COVID-19 cases per 10,000 persons for each of these zip codes.

Results: Indiana had a total of 3,892 positive COVID-19 cases from January 1 to April 30, 2020. The odds of testing positive for COVID-19 were four-fold higher in African Americans than non-African Americans (OR 4.58, 95% CI 4.25–4.94, P < 0.0001). Increased COVID-19 cases per 10,000 persons were seen in zip codes with higher percentage of African American (median infection rate of 17.4 per 10,000 population in zip codes above median % African American compared to 6.7 per 10,000 population in zip codes below median % African American, with an overall median infection rate 9.9 per 10,000 population, P < 0.0001) or Hispanic residents (median infection rate of 15.9 per 10,000 population in zip codes above median % Hispanic compared to 7.0 per 10,000 population in zip codes below median % Hispanic, overall median infection rate 9.6 per 10,000 population, P < 0.0001).

Conclusions: Individuals from zip codes with higher percentages of African American, Hispanic, foreign-born, and/or residents living in poverty are disproportionately affected by COVID-19. Urgent work is needed to understand and address the disproportionate burden of COVID-19 in minority communities and when economic disparities are present.
Groundwater–Stream Connectivity Mediates Metal(loid) Geochemistry in the Hyporheic Zone of Streams Impacted by Historic Mining and Acid Rock Drainage
Authors: Hoagland Beth , Navarre-Sitchler Alexis , Cowie Rory , Singha Kamini
Published: December 2020

High concentrations of trace metal(loid)s exported from abandoned mine wastes and acid rock drainage pose a risk to the health of aquatic ecosystems. To determine if and when the hyporheic zone mediates metal(loid) export, we investigated the relationship between streamflow, groundwater–stream connectivity, and subsurface metal(loid) concentrations in two ~1-km stream reaches within the Bonita Peak Mining District, a US Environmental Protection Agency Superfund site located near Silverton, Colorado, USA. The hyporheic zones of reaches in two streams—Mineral Creek and Cement Creek—were characterized using a combination of salt-tracer injection tests, transient-storage modeling, and geochemical sampling of the shallow streambed (<0.7 m). Based on these data, we present two conceptual models for subsurface metal(loid) behavior in the hyporheic zones, including (1) well-connected systems characterized by strong hyporheic mixing of infiltrating stream water and upwelling groundwater and (2) poorly connected systems delineated by physical barriers that limit hyporheic mixing. The comparatively large hyporheic zone and high hydraulic conductivities of Mineral Creek created a connected stream–groundwater system, where mixing of oxygen-rich stream water and metal-rich groundwater facilitated the precipitation of metal colloids in the shallow subsurface. In Cement Creek, the precipitation of iron oxides at depth (~0.4 m) created a low-hydraulic-conductivity barrier between surface water and groundwater. Cemented iron oxides were an important regulator of metal(loid) concentrations in this poorly connected stream–groundwater system due to the formation of strong redox gradients induced by a relatively small hyporheic zone and high fluid residence times. A comparison of conceptual models to stream concentration–discharge relationships exhibited a clear link between geochemical processes occurring within the hyporheic zone of the well-connected system and export of particulate Al, Cu, Fe, and Mn, while the poorly connected system did not have a notable influence on metal concentration–discharge trends. Mineral Creek is an example of a hyporheic system that serves as a natural dissolved metal(loid) sink, whereas poorly connected systems such as Cement Creek may require a combination of subsurface remediation of sediments and mitigation of upstream, iron-rich mine drainages to reduce metal export.
Caffeine and Exercise Performance: Possible Directions for Definitive Findings
Authors: Martins Gabriel Loureiro , Guilherme João Paulo Limongi França , Ferreira Luis Henrique Boiko , de Souza-Junior Tácito Pessoa , Lancha Antonio Herbert
Published: December 2020

Caffeine is one of the most studied supplements in the world. Studies correlate its use to increased exercise performance in endurance activities, as well as its possible ergogenic effects for both intermittent and strength activities. Recent findings show that caffeine may increase or decrease exercise performance. These antagonist responses may occur even when using the same dosage and for individuals with the same characteristics, making it challenging to explain caffeine's impact and applicability. This review article provides an analytic look at studies involving the use of caffeine for human physical performance, and addresses factors that could influence the ergogenic effects of caffeine on different proposed activities. These factors subdivide into caffeine effects, daily habits, physiological factors, and genetic factors. Each variable has been focused on by discussions to research related to caffeine. A better understanding and control of these variables should be considered in future research into personalized nutritional strategies.
A Comparison of Collection Methods for Microbial Volatiles
Authors: Rering Caitlin C. , Gaffke Alexander M. , Rudolph Arthur B. , Beck John J. , Alborn Hans T.
Published: December 2020

Recently, there has been an increase in the number of reports that highlight the role of microbes and their volatile metabolites in interactions with plants and insects, including interactions which may benefit agricultural production. Accurate and reproducible volatile collection is crucial to investigations of chemical-mediated communication between organisms. Accordingly, accurate detection of volatiles emitted from microbe-inoculated media is a research priority. Though numerous classes of volatile organic compounds are emitted from plants, insects, and microbes, emissions from microbes typically contain polar compounds of high volatility. Therefore, commonly used plant or insect volatile collection techniques may not provide an accurate representation of microbe-specific volatile profiles. Here, we present and compare the volatile data derived via three solventless collection techniques: direct headspace injection, solid-phase microextraction (SPME), and active sampling with a sorptive matrix blend specifically designed to prevent collection filter breakthrough of VOCs (solid-phase extraction, SPE). These methods were applied to a synthetic floral nectar media containing a nectar-inhabiting yeast, Metschnikowia reukaufii, and sunflower (Helianthus annus) pollen. The yeast contributed alcohols, ketones, and esters, and the pollen provided terpenoids. Direct headspace injections were not effective, and the resultant chromatography was poor despite the use of on-column cryofocusing. SPME and SPE detected a similar number of volatiles, but with varying relative abundances. SPE collected a greater abundance of microbial volatiles than SPME, a difference driven by high ethanol capture in SPE. Both SPE and SPME are appropriate for analysis of microbial volatiles, though the sorbent type and amount, and other collection parameters should be further evaluated for each studied system.
Growth of Cucumber Scions, Rootstocks, and Grafted Seedlings as Affected by Different Irrigation Regimes during Cultivation of ‘Joenbaekdadagi’ and ‘Heukjong’ Seedlings in a Plant Factory with Artificial Lighting
Authors: An Sewoong , Park Seon Woo , Kwack Yurina
Published: December 2020

Irrigation scheduling and programming are very effective tools for efficient water use in a plant factory with artificial lighting (PFAL). In order to confirm optimal irrigation schemes for the production of cucumber scions and rootstocks in a PFAL, in this study, four different start points of irrigation were applied by measuring the weight of the plug tray to compare the growth of cucumber scions and rootstocks cultivated in a PFAL. Additionally, the growth characteristics of cucumber seedlings grafted with scions and rootstocks cultivated between in a greenhouse and in a PFAL were investigated. Although the growth of cucumber scions and rootstocks was highest when irrigation was conducted at 70% of water content in a medium, the growth of grafted cucumber seedlings before and after transplanting was not significantly different among the irrigation treatments in a PFAL. However, water use efficiency (WUE) during cucumber scions and rootstock production in a PFAL was higher at 60% than at 70%. Considering seedling growth and the efficiency of irrigation such as WUE and irrigation schedule, the optimal start point of irrigation during the production of cucumber scions and rootstocks in a PFAL was determined as 60% of water content in a medium. When the optimal irrigation regime was applied to the production of cucumber scions and rootstocks in a PFAL, the morphological characteristics of cucumber scions and rootstocks cultivated in a PFAL were more suitable for grafting compared with that of the cucumber scions and rootstocks cultivated conventionally in a greenhouse. The favorable environmental conditions during the cultivation of cucumber scions and rootstocks in a PFAL also positively affected the flowering response of cucumber grafted seedlings after transplanting.
Carbon Isotope Measurements to Determine the Turnover of Soil Organic Matter Fractions in a Temperate Forest Soil
Authors: Zacháry Dóra , Filep Tibor , Jakab Gergely , Molnár Mihály , Kertész Titanilla , Király Csilla , Hegyi István , Gáspár Lilla , Szalai Zoltán
Published: December 2020

Soil organic matter (SOM) is a combination of materials having different origin and with different stabilization and decomposition processes. To determine the different SOM pools and their turnover rates, a silt loam-textured Luvisol from West Hungary was taken from the 0–20 cm soil depth and incubated for 163 days. Maize residues were added to the soil in order to obtain natural 13C enrichment. Four different SOM fractions—particulate organic matter (POM), sand and stable aggregate (S + A), silt- plus clay-sized (s + c) and chemically resistant soil organic carbon (rSOC) fractions—were separated and analyzed using FT-IR, δ13C, and 14C measurements. The mean residence time (MRT) of the new C and the proportion of maize-derived C in the fractions were calculated. The POM fraction was found to be the most labile C pool, as shown by the easily decomposable chemical structures (e.g., aliphatic, O-alkyl, and polysaccharides), the highest proportion (11.7 ± 2.5%) of maize-derived C, and an MRT of 3.6 years. The results revealed that the most stable fraction was the rSOC fraction which had the smallest proportion of maize-derived C (0.18 ± 2.5%) and the highest MRT (250 years), while it was the only fraction with a negative value of Δ14C (−75.0 ± 2.4‰). Overall, the study confirmed the hypothesis that the SOM associated with finer-sized soil particles decomposes the least, highlighting the significance of the fractionation process for more accurate determination of the decomposition processes of SOM pools.
Correction: Jansen, S., et al. Relationship between Bone Stability and Egg Production in Genetically Divergent Chicken Layer Lines. Animals 2020, 10, 850
Authors: Jansen Simon , Baulain Ulrich , Habig Christin , Weigend Annett , Halle Ingrid , Scholz Armin Manfred , Simianer Henner , Sharifi Ahmad Reza , Weigend Steffen
Published: December 2020

The authors wish to make the following corrections to this paper [...]
Ultrasonographic Algorithm for the Assessment of Sentinel Lymph Nodes That Drain the Mammary Carcinomas in Female Dogs
Authors: Stan Florin , Gudea Alexandru , Damian Aurel , Gal Adrian Florin , Papuc Ionel , Pop Alexandru Raul , Martonos Cristian
Published: December 2020

The status of sentinel lymph nodes (SLNs) is decisive in staging, prognosis, and therapeutic approach. Using an ultrasonographic examination algorithm composed of B-mode, Doppler technique, contrast-enhanced ultrasound (CEUS) and elastography, this study aimed to determine the diagnostic performance of the four techniques compared to histopathological examination. 96 SLNs belonging to 71 female dogs with mammary gland carcinomas were examined. After examinations, mastectomy and lymphadenectomy were performed. Histopathological examination confirmed the presence of metastases in 54 SLNs. The elasticity score had the highest accuracy—89.71%, identifying metastases in SLNs with 88.9.9% sensitivity (SE) and 90.5% specificity (SP), ROC analysis providing excellent results. The S/L (short axis/long axis) ratio showed 83.3% SE and 78.6% SP as a predictor of the presence of metastases in SLN having a good accuracy of 81.2%. On Doppler examination, the resistivity index(RI) showed good accuracy of 80% in characterizing lymph nodes with metastases versus unaffected ones; the same results being obtained by CEUS examination. By assigning to each ultrasonographic parameter a score (0 or 1) and summing up the scores of the four techniques, we obtained the best diagnostic performance in identifying lymph node metastases with 92.2% accuracy. In conclusion, the use of the presented algorithm provides the best identification of metastases in SLNs, helping in mammary carcinoma staging and appropriate therapeutic management.
Genetic and Non-Genetic Variation of Milk Total Antioxidant Activity Predicted from Mid-Infrared Spectra in Holstein Cows
Authors: Niero Giovanni , Costa Angela , Franzoi Marco , Visentin Giulio , Cassandro Martino , De Marchi Massimo , Penasa Mauro
Published: December 2020

Food antioxidants enhance products shelf life and stability during technological treatments through the maintenance of their physical and chemical properties. Moreover, they are endowed with several positive effects on human health, including cell membranes preservation, enzyme functionality, and DNA integrity. Milk has been described in relation to a wide array of fat soluble and water-soluble antioxidant compounds, in particular vitamin A, C, and E, lactoferrin and peptides derived from casein and whey proteins. The total antioxidant activity (TAA) of milk is a novel and scarcely explored trait, defined as the sum of antioxidant contributions of the aforementioned compounds. On this background, the aims of the present study were to investigate the variability of milk TAA on a large scale exploiting predictions obtained through mid-infrared (MIR) spectroscopy and to estimate genetic parameters of this trait in Holstein cows. Individual milk samples were collected between January 2011 and December 2018 during the routine milk recording procedure. Samples were analysed for gross composition through MIR spectroscopy and MIR spectra were stored. Milk TAA was then predicted (pTAA) from the stored milk MIR spectra (111,653 test-day records of 9519 cows in 344 herds) using the previously developed prediction model; considering the prediction accuracy, pTAA might be considered a proxy of the TAA determined through the reference method. Overall, pTAA averaged 7.16 mmoL/L of Trolox equivalents, showed a nadir around 40 days after calving and increased thereafter, following a linear trend up to the end of lactation. The lowest pTAA was observed in milk sampled from June to September. Milk pTAA was heritable (0.401 ± 0.015) and genetically associated to fat yield (0.366 ± 0.049), crude protein (CP) yield (0.238 ± 0.052), fat percentage (0.616 ± 0.022) and CP percentage (0.754 ± 0.015). The official selection index of Italian Holstein put the 49% of the emphasis on fat and protein yield and percentage; therefore, it derives that an indirect favourable selection for milk pTAA should be already in progress in Italian Holstein population.
Design and Experimentation of an Aerial Seeding System for Rapeseed Based on an Air-Assisted Centralized Metering Device and a Multi-Rotor Crop Protection UAV
Authors: Huang Xiaomao , Zhang Shun , Luo Chengming , Li Wencheng , Liao Yitao
Published: December 2020

To improve the overall mechanization level of rapeseed production in China, especially in some hilly regions where ground machinery cannot enter the fields or can only enter with very low economic benefits, a special aerial seeding system for rapeseed based on a miniature air-assisted centralized metering device was designed and tested in this study. Unlike existing commercial aerial seeding systems, the proposed seed meter was a miniaturized version derived from the traditional air-feeding seed meter on ground planters. The new version contained a redesigned seed feeding component to overcome problems of serious air backflow to the seed box and difficult seed feeding after miniaturization. Three groups of experiments were designed and conducted to optimize the parameters of the seed meter and test its performance. Results from the orthogonal experiment showed that the seed feeding component performed best when the seed layer thickness was 45 mm, the rotational speed of the gear disc was 45 r/min, and the airflow pressure was 2450 Pa. Results from the static workbench test showed that the designed seeding system had a maximum average total sowing efficiency of 537.17 g/min, with the maximum values of the stability variation coefficient of total seeding rate (seven ports) and the consistency variation coefficient between each port was 2.37% and 4.89%, respectively. Field tests further proved that the designed aerial seeding system could work stably, uniformly, and efficiently, so that the agronomic requirements of rape crop planting could be well met.
Macroplastic in Seabirds at Mirny, Antarctica
Authors: Golubev Sergey
Published: December 2020

Plastic debris makes up the majority of marine debris around the world, and pollution is a serious threat to marine wildlife. Threats represent two types of biological interactions with plastic: entanglement and ingestion. This paper describes interactions of seabirds with plastic in Mirny and draw the attention of researchers to the existing problem. In 2012/2013 and 2015/2016, year-round observations of the author were carried out at Mirny station and Haswell Islands (area of about 12 km2), east Antarctica. One case of entanglement of a molting adult Adélie penguin (Pygoscelis adeliae) in a fishing line was been identified; in addition to one case of an adult emperor penguin (Aptenodytes forsteri) mistakenly ingesting plastic braided rope and subsequently feeding it as part of a food lump to the chick, and two cases of macroplastics found in pellets of south polar skuas (Catharacta maccormicki). Registrations of entanglement and ingestion of macroplastic by seabirds in Mirny are rare. They signal to us about problem that needs to be included in the monitoring for the health of terrestrial biota of the Haswell archipelago.
Common Factors in Neurodegeneration: A Meta-Study Revealing Shared Patterns on a Multi-Omics Scale
Authors: Ruffini Nicolas , Klingenberg Susanne , Schweiger Susann , Gerber Susanne
Published: December 2020

Neurodegenerative diseases such as Alzheimer’s disease (AD), Parkinson’s disease (PD), Huntington’s disease (HD), and amyotrophic lateral sclerosis (ALS) are heterogeneous, progressive diseases with frequently overlapping symptoms characterized by a loss of neurons. Studies have suggested relations between neurodegenerative diseases for many years (e.g., regarding the aggregation of toxic proteins or triggering endogenous cell death pathways). We gathered publicly available genomic, transcriptomic, and proteomic data from 177 studies and more than one million patients to detect shared genetic patterns between the neurodegenerative diseases on three analyzed omics-layers. The results show a remarkably high number of shared differentially expressed genes between the transcriptomic and proteomic levels for all conditions, while showing a significant relation between genomic and proteomic data between AD and PD and AD and ALS. We identified a set of 139 genes being differentially expressed in several transcriptomic experiments of all four diseases. These 139 genes showed overrepresented gene ontology (GO) Terms involved in the development of neurodegeneration, such as response to heat and hypoxia, positive regulation of cytokines and angiogenesis, and RNA catabolic process. Furthermore, the four analyzed neurodegenerative diseases (NDDs) were clustered by their mean direction of regulation throughout all transcriptomic studies for this set of 139 genes, with the closest relation regarding this common gene set seen between AD and HD. GO-Term and pathway analysis of the proteomic overlap led to biological processes (BPs), related to protein folding and humoral immune response. Taken together, we could confirm the existence of many relations between Alzheimer’s disease, Parkinson’s disease, Huntington’s disease, and amyotrophic lateral sclerosis on transcriptomic and proteomic levels by analyzing the pathways and GO-Terms arising in these intersections. The significance of the connection and the striking relation of the results to processes leading to neurodegeneration between the transcriptomic and proteomic data for all four analyzed neurodegenerative diseases showed that exploring many studies simultaneously, including multiple omics-layers of different neurodegenerative diseases simultaneously, holds new relevant insights that do not emerge from analyzing these data separately. Furthermore, the results shed light on processes like the humoral immune response that have previously been described only for certain diseases. Our data therefore suggest human patients with neurodegenerative diseases should be addressed as complex biological systems by integrating multiple underlying data sources.
Retinoic Acid and Its Derivatives in Skin
Authors: Szymański Łukasz , Skopek Rafał , Palusińska Małgorzata , Schenk Tino , Stengel Sven , Lewicki Sławomir , Kraj Leszek , Kamiński Paweł , Zelent Arthur
Published: December 2020

The retinoids are a group of compounds including vitamin A and its active metabolite all-trans-retinoic acid (ATRA). Retinoids regulate a variety of physiological functions in multiple organ systems, are essential for normal immune competence, and are involved in the regulation of cell growth and differentiation. Vitamin A derivatives have held promise in cancer treatment and ATRA is used in differentiation therapy of acute promyelocytic leukemia (APL). ATRA and other retinoids have also been successfully applied in a variety of dermatological conditions such as skin cancer, psoriasis, acne, and ichthyosis. Moreover, modulation of retinoic acid receptors and retinoid X (or rexinoid) receptors function may affect dermal cells. The studies using complex genetic models with various combinations of retinoic acid receptors (RARs) and retinoid X (or rexinoid) receptors (RXRs) indicate that retinoic acid and its derivatives have therapeutic potential for a variety of serious dermatological disorders including some malignant conditions. Here, we provide a synopsis of the main advances in understanding the role of ATRA and its receptors in dermatology.
Hypotheses for the Reasons behind Beer Consumer’s Willingness to Purchase Beer: An Expanded Theory from a Planned Behavior Perspective
Authors: Wang Edward Shih-Tse
Published: December 2020

Because beer is one of the most common alcoholic beverages consumed in the world, this research adopted an expanded theory of planned behavior (TPB) perspective to understand why beer consumers purchase beer. This study investigated the effects of injunctive norms, descriptive norms, attitude, and perceived behavioral control on alcohol identity and purchase intention. The possible mediating role of alcohol identity was also investigated. This study was conducted in Taiwan, and a total of 452 beer consumers participated in the survey. Structural equation modeling was used to examine the relationship among the study variables. The results revealed that alcohol identity positively influences purchase intention, and attitude positively affects alcohol identity and purchase intention. In addition, injunctive norms have a positive influence on alcohol identity, and descriptive norms positively affect purchase intention. In particular, perceived behavioral control has a negative influence on alcohol identity but has a positive influence on purchase intention. This study also found that alcohol identity mediates the attitude–purchase intention relationship. By examining the consumption behavior of beer consumers from the TPB perspective, this study contributed to an understanding of beer consumption behavior.
Wage Differences Matter: An Experiment of Social Comparison and Effort Provision when Wages Increase or Decrease
Authors: Rojas-Fallas Jose , Williams J. Forrest
Published: December 2020

Wage rates, efficiency wages, and gift exchange in a labor market are all crucial aspects in regard to designing contracts to ensure high effort from workers. We extend this literature by discussing the relationship between known differences in wages (social comparison) and workers’ effort provision. We conduct an experiment in which subjects perform effort tasks for piece-rates. All subjects are paid the same wage rate in the first half of the experiment, but in the second half are paid different wage rates; the primary variable we study is the information about others’ wage rates given to a subset of subjects. We find that subjects’ efforts respond strongly to information about others’ wages. Such findings have implications for contract structuring for workers.
Novel Molecular Evidence Related to COVID-19 in Patients with Diabetes Mellitus
Authors: Liao Yu-Huang , Zheng Jing-Quan , Zheng Cai-Mei , Lu Kuo-Cheng , Chao You-Chen
Published: December 2020

Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has rapidly evolved into a global pandemic. The hyperglycemia in patients with diabetes mellitus (DM) substantially compromises their innate immune system. SARS-CoV-2 uses human angiotensin-converting enzyme 2 (ACE2) receptors to enter the affected cell. Uncontrolled hyperglycemia-induced glycosylation of ACE2 and the S protein of SARS-CoV-2 could facilitate the binding of S protein to ACE2, enabling viral entry. Downregulation of ACE2 activity secondary to SARS-CoV-2 infection, with consequent accumulation of angiotensin II and metabolites, eventually leads to poor outcomes. The altered binding of ACE2 with SARS-CoV-2 and the compromised innate immunity of patients with DM increase their susceptibility to COVID-19; COVID-19 induces pancreatic β-cell injury and poor glycemic control, which further compromises the immune response and aggravates hyperglycemia and COVID-19 progression, forming a vicious cycle. Sequential cleavage of viral S protein by furin and transmembrane serine protease 2 (TMPRSS2) triggers viral entry to release the viral genome into the target cell. Hence, TMPRSS2 and furin are possible drug targets. As type 1 DM exhibits a Th1-driven autoimmune process, the relatively lower mortality of COVID-19 in type 1 DM compared to type 2 DM might be attributed to an imbalance between Th1 and Th2 immunity. The anti-inflammatory effects of dipeptidyl peptidase-4 inhibitor may benefit patients with DM and COVID-19. The potential protective effects of sodium–glucose cotransporter-2 inhibitor (SGLT2i), including reduction in lactate level, prevention of lowering of cytosolic pH and reduction in pro-inflammatory cytokine levels may justify the provision of SGLT2i to patients with DM and mild or asymptomatic COVID-19. For patients with DM and COVID-19 who require hospitalization, insulin-based treatment is recommended with cessation of metformin and SGLT2i. Further evidence from randomized or case–control clinical trials is necessary to elucidate the effectiveness and pitfalls of different types of medication for DM.
Changes in the Salivary Metabolic Profile of Generalized Periodontitis Patients after Non-surgical Periodontal Therapy: A Metabolomic Analysis Using Nuclear Magnetic Resonance Spectroscopy
Authors: Citterio Filippo , Romano Federica , Meoni Gaia , Iaderosa Giovanni , Grossi Silvia , Sobrero Alberto , Dego Francesca , Corana Matteo , Berta Giovanni Nicolao , Tenori Leonardo , Aimetti Mario
Published: December 2020

Pattern analysis of the salivary metabolic profile has been proven accurate in discriminating between generalized periodontitis (GP) patients and healthy individuals (HI), as this disease modifies the salivary concentrations of specific metabolites. Due to the scarcity of data from previous studies, this study aimed to evaluate if non-surgical periodontal therapy (NST) could affect the metabolomic profile in GP patients’ saliva and if it compares to that of HI. Unstimulated salivary samples were collected from 11 HI and 12 GP patients before and 3 months after NST. Nuclear Magnetic Resonance (NMR) spectroscopy, followed by a supervised multivariate statistical approach on entire saliva spectra and partial least square (PLS) discriminant analysis, were performed to obtain metabolic profiles. In the GP group, periodontal treatment improved all clinical parameters, but not all the diseased sites were eradicated. PLS revealed an accuracy of 100% in distinguishing between metabolic profiles of GP patients before and after NST. Orthogonal projection to latent structure was able to discriminate between the three groups of subjects with an accuracy of 85.6%. However, the post-NST metabolic profile of GP patients could not be completely assimilated to that of HI. Although NST may produce significant changes in the metabolic profile, GP patients maintained a distinctive fingerprint compared to HI.
Kinesiology Taping as an Innovative Measure against Post-Operative Complications after Third Molar Extraction—Systematic Review
Authors: Jaroń Aleksandra , Jedliński Maciej , Grzywacz Elżbieta , Mazur Marta , Trybek Grzegorz
Published: December 2020

There are many randomized clinical trials suggesting a positive effect of kinesiotaping on postoperative swelling. In dentistry, however, the use of kinesiotaping still seems to be innovative, since not many articles on kinesiotaping within the craniofacial area have been published. This study aimed to systematically review and synthesize available controlled trials examining the use of kinesiotaping to reduce morbidity after third molar extraction. Literature searches for free text and MeSH terms were performed using five search engines, and used to find studies which focused on kinesiotaping as a form of rehabilitation after third molar extraction. The keywords used in the search were: “((“molar, third”[MeSH Terms] OR (“molar”[All Fields] AND “third”[All Fields]) OR “third molar”[All Fields] OR (“third”[All Fields] AND “molar”[All Fields])) AND extraction [All Fields]) AND “kinesiology”[All Fields]”. For the assessment of the risk of bias, the Jadad and Maastricht scales were applied. The search strategy identified 317 potential articles. After analysis, 10 papers were included in the final evaluation. Despite the fact that most of the included articles adhered to methodological standards, the fact that there are only a few of them points to a further need for scientific development of physiotherapy in this regard. Kinesiology taping is useful against post-operative morbidity of the third molar extraction site. The present studies show a low level of the risk of bias, but they are limited in number; therefore, it seems that more research is needed.
Long-Term Surgical Results of Skip Pedicle Screw Fixation for Patients with Adolescent Idiopathic Scoliosis: A Minimum-Ten-Year Follow-Up Study
Authors: Uehara Masashi , Kuraishi Shugo , Ikegami Shota , Oba Hiroki , Takizawa Takashi , Munakata Ryo , Hatakenaka Terue , Koseki Michihiko , Takahashi Jun
Published: December 2020

Skip pedicle screw fixation for adolescent idiopathic scoliosis (AIS) requires fewer screws and can reduce the risk of neurovascular injury as compared with segmental pedicle screw fixation. However, the long-term impact of screw number reduction on correction and clinical results is unclear. This study examined the 10-year post-operative outcomes of skip pedicle screw fixation for patients with AIS. We reviewed the outcomes of 30 patients who underwent skip pedicle screw fixation for AIS. Radiological and clinical findings were assessed before and immediately, 2 years, and 10 years after surgery in the remaining 25 patients. The mean Cobb angle of the main curve preoperatively and immediately, 2 years, and 10 years post-operatively was 59.4°, 23.4°, 25.8°, and 25.60°, respectively, and was significantly improved at all post-surgical time points (all p < 0.001). The mean correction rate immediately after surgery was 60.8%, and the correction loss rate at the observation end point was 4.8%. The Cobb angle of the lumbar curve was significantly improved immediately after surgery, and the correction persisted until 10 years post-operatively. Remarkable gains were observed for most Scoliosis Research Society-22 patient questionnaire sub-scores at the final follow-up versus preoperative assessments. In conclusion, good correction of the AIS deformity by skip pedicle screw fixation was well maintained over a long follow-up period of 10 years, with clinically meaningful gains in Society-22 patient questionnaire sub-scores.
Kinetic GFR Outperforms CKD-EPI for Slow Graft Function Prediction in the Immediate Postoperative Period Following Kidney Transplantation
Authors: Dash Jonathan , Verissimo Thomas , Faivre Anna , Berchtold Lena , Berney Thierry , Pugin Jérôme , de Seigneux Sophie , Legouis David
Published: December 2020

Background: Rapid identification of patients at high risk for slow graft function (SGF) is of major importance in the immediate period following renal graft transplantation, both for early therapeutic decisions and long-term prognosis. Due to the high variability of serum creatinine levels after surgery, glomerular filtration rate (GFR) estimation is challenging. In this situation, kinetic estimated GFR (KeGFR) equations are interesting tools but have never been assessed for the identification of SGF patients. Methods: We conducted a single-center retrospective cohort study, including all consecutive kidney allograft recipients in the University Hospitals of Geneva from 2008 to 2016. GFR was estimated using both CKD-EPI and KeGFR formulae. Their accuracies for SGF prediction were compared. Patients were followed up for one year after transplantation. Results: A total of 326 kidney recipients were analyzed. SGF occurred in 76 (23%) patients. KeGFR estimation stabilized from the day following kidney transplantation, more rapidly than CKD-EPI. Discrimination ability for SGF prediction was better for KeGFR than CKD-EPI (AUC 0.82 and 0.66, p < 0.001, respectively). Conclusion: KeGFR computed from the first day after renal transplantation was able to predict SGF with good discrimination, outperforming CKD-EPI estimation. SGF patients had lower renal graft function overall at the one-year follow up.
Porphyromonas gingivalis Mfa1 Induces Chemokine and Cell Adhesion Molecules in Mouse Gingival Fibroblasts via Toll-Like Receptors
Authors: Takayanagi Yuhei , Kikuchi Takeshi , Hasegawa Yoshiaki , Naiki Yoshikazu , Goto Hisashi , Okada Kousuke , Okabe Iichiro , Kamiya Yosuke , Suzuki Yuki , Sawada Noritaka , Okabe Teppei , Suzuki Yuki , Kondo Shun , Ohno Tasuku , Hayashi Jun-Ichiro , Mitani Akio
Published: December 2020

Porphyromonas gingivalis Mfa1 fimbriae are thought to act as adhesion factors and to direct periodontal tissue destruction but their immunomodulatory actions are poorly understood. Here, we investigated the effect of Mfa1 stimulation on the immune and metabolic mechanisms of gingival fibroblasts from periodontal connective tissue. We also determined the role of Toll-like receptor (TLR) 2 and TLR4 in Mfa1 recognition. Mfa1 increased the expression of genes encoding chemokine (C-X-C motif) ligand (CXCL) 1, CXCL3, intercellular adhesion molecule (ICAM) 1 and Selectin endothelium (E) in gingival fibroblasts, but did not have a significant effect on genes that regulate metabolism. Mfa1-stimulated up-regulation of genes was significantly suppressed in Tlr4 siRNA-transfected cells compared with that in control siRNA-transfected cells, which indicates that recognition by TLR4 is essential for immunomodulation by Mfa1. Additionally, suppression of Tlr2 expression partially attenuated the stimulatory effect of Mfa1. Overall, these results help explain the involvement of P. gingivalis Mfa1 fimbriae in the progression of periodontal disease.
A Computer-Aided Drug Design Approach to Predict Marine Drug-Like Leads for SARS-CoV-2 Main Protease Inhibition
Authors: Gaudêncio Susana P. , Pereira Florbela
Published: December 2020

The investigation of marine natural products (MNPs) as key resources for the discovery of drugs to mitigate the COVID-19 pandemic is a developing field. In this work, computer-aided drug design (CADD) approaches comprising ligand- and structure-based methods were explored for predicting SARS-CoV-2 main protease (Mpro) inhibitors. The CADD ligand-based method used a quantitative structure–activity relationship (QSAR) classification model that was built using 5276 organic molecules extracted from the ChEMBL database with SARS-CoV-2 screening data. The best model achieved an overall predictive accuracy of up to 67% for an external and internal validation using test and training sets. Moreover, based on the best QSAR model, a virtual screening campaign was carried out using 11,162 MNPs retrieved from the Reaxys® database, 7 in-house MNPs obtained from marine-derived actinomycetes by the team, and 14 MNPs that are currently in the clinical pipeline. All the MNPs from the virtual screening libraries that were predicted as belonging to class A were selected for the CADD structure-based method. In the CADD structure-based approach, the 494 MNPs selected by the QSAR approach were screened by molecular docking against Mpro enzyme. A list of virtual screening hits comprising fifteen MNPs was assented by establishing several limits in this CADD approach, and five MNPs were proposed as the most promising marine drug-like leads as SARS-CoV-2 Mpro inhibitors, a benzo[f]pyrano[4,3-b]chromene, notoamide I, emindole SB beta-mannoside, and two bromoindole derivatives.