bluuebunny/crossref_metadata_2025 huggingface dataset:
Functions of Music Making Under Lockdown: A Trans-Historical Perspective Across Two Pandemics
Authors: Chiu Remi
Published: January 2021

This paper describes how music fulfills two of its broadly recognized functions—“mood regulation” and “social cohesion”—in times of pandemics and social isolation. Through a trans-historical comparison of the musical activities of the Milanese during an outbreak of plague in 1576 with the musical activities observed during the COVID lockdowns in 2020 (such as balcony-singing and playlist-making), this paper suggests a framework for understanding the role of music in the care of the biological body and the social body in times of medical disaster.
Machine Gaze: Self-Identification Through Play With a computer Vision-Based Projection and Robotics System
Authors: LC RAY , Alcibar Aaliyah , Baez Alejandro , Torossian Stefanie
Published: January 2021

Children begin to develop self-awareness when they associate images and abilities with themselves. Such “construction of self” continues throughout adult life as we constantly cycle through different forms of self-awareness, seeking, to redefine ourselves. Modern technologies like screens and artificial intelligence threaten to alter our development of self-awareness, because children and adults are exposed to machines, tele-presences, and displays that increasingly become part of human identity. We use avatars, invent digital lives, and augment ourselves with digital imprints that depart from reality, making the development of self-identification adjust to digital technologies that blur the boundary between us and our devices. To empower children and adults to see themselves and artificially intelligent machines as separately aware entities, we created the persona of a salvaged supermarket security camera refurbished and enhanced with the power of computer vision to detect human faces, and project them on a large-scale 3D face sculpture. The surveillance camera system moves its head to point to human faces at times, but at other times, humans have to get its attention by moving to its vicinity, creating a dynamic where audiences attempt to see their own faces on the sculpture by gazing into the machine's eye. We found that audiences began attaining an understanding of machines that interpret our faces as separate from our identities, with their own agendas and agencies that show by the way they serendipitously interact with us. The machine-projected images of us are their own interpretation rather than our own, distancing us from our digital analogs. In the accompanying workshop, participants learn about how computer vision works by putting on disguises in order to escape from an algorithm detecting them as the same person by analyzing their faces. Participants learn that their own agency affects how machines interpret them, gaining an appreciation for the way their own identities and machines' awareness of them can be separate entities that can be manipulated for play. Together the installation and workshop empower children and adults to think beyond identification with digital technology to recognize the machine's own interpretive abilities that lie separate from human being's own self-awareness.
Covid-19 and the Brazilian Reality: The Role of Favelas in Combating the Pandemic
Authors: Fernandes Luana Almeida de Carvalho , Silva Caíque Azael Ferreira da , Dameda Cristiane , Bicalho Pedro Paulo Gastalho de
Published: January 2021

The consequences of coronavirus in favelas in Rio de Janeiro (Brazil) point to social inequality as a structuring factor in Brazilian society. The contagion spread and multiple death cases reveal the multiplicity of existence ways that cohabit the urban context, indicating that in many of these scenarios, access to decent housing, drinking water, and minimum income is not a reality and recommendations from international health agencies are challenging to implement. Against government technopolitics that drive different forms of death to the poorest, black communities, and slum dwellers, territorial insurgencies indicate other paths for the construction of a dignified life and access to fundamental rights, targeted solidarity practices, territorial political organization and the construction of specific public policies to deal with the effects of the virus which takes into account the particularities and distinct realities of the territory. The experiences of community organization around Crisis Offices in the favelas, led by social organizations and supporting institutions, have guaranteed (i) food and personal hygiene items distribution, (ii) sanitization of alleys, (iii) dissemination of information on the virus, and (iv) political articulation for disputes in defense of life preservation in the favelas, in opposition of genocidal processes carried out by the state power. Such local spaces represent practices of resistance to the death policies undertaken by the state policies, which most are not configured as spaces for collective construction and disregard inequalities and different needs in these territories. That way, community associations are presented as an inflection point, a deviation from the normal course of modulated subjectivities by the social principles and practices of neoliberalism, with the indication that the most efficient way to deal with social crises is through the strengthening of the collective and the popular organizations.
Low-Field Magnetic Resonance Imaging Findings in 18 Dogs With Presumed Optic Neuritis
Authors: Muñiz Moris Laura , Cherubini Giunio Bruto , Caine Abby
Published: January 2021

Canine optic neuritis has been attributed to a focal or disseminated form of granulomatous meningoencephalitis (GME) amongst other etiologies. Magnetic resonance imaging (MRI) has been proven to help differentiate the structures within the optic nerve sheath and therefore could aid the diagnosis of optic neuritis in dogs. The objectives of this study were to describe and compare the MRI abnormalities affecting the optic nerve sheath complex and optic chiasm in dogs with clinically suspected optic neuritis as a component of meningoencephalitis of unknown etiology (MUE) or as an isolated form (I-ON). Retrospective evaluation of patient details, clinical signs, cerebrospinal fluid (CSF) analysis, and MRI findings of dogs with clinically suspected optic neuritis between January 2011 and May 2018 was performed. Eighteen dogs met the inclusion criteria. MRI findings included contrast enhancement of both optic nerves (11/18) and optic chiasm (6/18), changes within the CSF volume surrounding the optic nerve (10/18), changes to the optic disc (10/18), changes of size or signal affecting the optic chiasm (10/18), changes in the Short TI inversion recovery (STIR) signal of the optic nerve (7/15), retrobulbar changes (3/18), and concurrent brain lesions (13/18). A variety of subtle MRI features may indicate optic nerve involvement and low-field MRI is a sensitive method to detect changes within the optic nerve sheath complex in dogs with optic neuritis as an isolated form (I-ON) or as an extension of MUE.
Porcine Circovirus Type 3 Cap Inhibits Type I Interferon Induction Through Interaction With G3BP1
Authors: Zhang Pengfei , Shen Hanqin , Liu Xianhui , Wang Shuangyun , Liu Yanling , Xu Zheng , Song Changxu
Published: January 2021

Porcine circovirus 3 (PCV3) infections cause clinical diseases similar to those seen in porcine circovirus 2 (PCV2) infections. It is unclear whether PCV3 infections can also cause immunosuppression like that seen with PCV2. Here, we report that Cap inhibits DNA-induced IFN-β mRNA transcription and IFN promoter activation. Cap was also found to inhibit cyclic GMP-AMP (cGAMP) synthase (cGAS) binding to interferon-stimulating DNA (ISD). Immunoprecipitation and mass spectrometry were used to identify cellular interaction partners of Cap. Cap interacted with G3BP1 and inhibited the interaction between GTPase-activating protein-(SH3 domain)-binding protein 1 (G3BP1) and cGAS. Furthermore, the destruction of endogenously expressed G3BP1 by siRNA significantly reduced IFN promoter activation, and phosphorylation of tank-binding kinase 1 (TBK1) was induced by ISD. Overexpression of G3BP1 attenuated the inhibition of ISD binding of cGAS by Cap and promoted phosphorylation of TBK1 and IRF3 induced by ISD. Collectively, our results show that the interaction between Cap and G3BP1 prevents cGAS from recognizing DNA, thereby inhibiting the IFN production.
Slightly and Moderately Lame Cows in Tie Stalls Behave Differently From Non-lame Controls. A Matched Case-Control Study
Authors: Bernhard Johanna Karin , Vidondo Beatriz , Achermann Rahel Lisa , Rediger Rahel , Stucki Dimitri , Müller Kerstin Elisabeth , Steiner Adrian
Published: January 2021

Lameness affects dairy cows worldwide and is usually associated with pain. Behavioral differences in lame compared to non-lame tie-stall-housed dairy cows might be less pronounced than in free-stall-housed, since the principle demands to a cow's locomotor system and thus the impact of lameness on behavior seem to be lower in tie stalls. Behavioral differences between lame and non-lame cows might be used to estimate the impact of lameness on the well-being of tied dairy cows. In the current study, lame cows were categorized as locomotion scoring between 2.25 and 3.25 on a 1–5 scale. The aim was to compare the eating, rumination and lying behavior of lame cows against non-lame tied dairy cows, in order to draw conclusions on the association of lameness, behavior and well-being in tied dairy cows. The eating and rumination behavior of 26, the lying behavior of 30, and the relative upright and lying activities of 25 matched case-control pairs were analyzed, considering the matching criteria farm, breed-type, and parity-group. Lame cows had fewer [mean of the pairwise differences (case–control) (meandiff) = −2.6 bouts, CI95% (−3.8–−1.4) bouts, p = 0.001], but longer lying bouts [meandiff = 26.7 min per bout, CI95% (10.1–43.4) min per bout, p = 0.006]. The lying time was shorter [meandiff = −64.7 min, CI95% (−104.4–−24.9) min, p = 0.006] in lame cows compared to their non-lame controls. Lame cows had a shorter eating time [meandiff = −27.7 min, CI95% (−51.5–−4.0) min, p = 0.042] and spent a larger proportion of their upright time ruminating [meandiff = 7.2%, CI95% (3.2–11.1)%, p = 0.001] instead of eating. The results of the current study indicate that the eating, rumination, and lying behavior of lame tied dairy cows is altered. These findings indicate that slight and moderate lameness (locomotion score between 2.25 and 3.25 on a 1–5 scale) are likely to be associated with an impaired well-being in affected tied dairy cows. This underlines the need to continuously reduce the lameness prevalence and severity in tied dairy herds.
Design and Manufacture of Composite Landing Gear for a Light Unmanned Aerial Vehicle
Authors: Liang Yen-Chu , Chin Pei-Chieh , Sun Yun-Ping , Wang Muh-Rong
Published: January 2021

Unmanned aerial vehicles (UAVs) have become popular for military applications as well as for use in structural inspection, weather monitoring, and festival demonstrations. Suitable landing gears to ensure that the UAV can take off and land safely are important when the price of the cargo and UAV increase. A lightweight and high-strength landing gear is desirable to overcome accidents during service. Composite materials are a promising, excellent solution for this purpose. This study demonstrated the design, numerical analysis (Ansys), manufacture, and experimental verification (LabVIEW) of a composite landing gear for a UAV. In particular, landing gears with different carbon fiber-reinforced polymer (CFRP) composite fiber orientations were analyzed, manufactured, and experimentally verified in this study.
Comparison of CFD and Multizone Modeling from Contaminant Migration from a Household Gas Furnace
Authors: Szczepanik-Scislo Nina , Scislo Lukasz
Published: January 2021

In Central and Eastern Europe, a growing popularity of gas heaters as the main source of heat and domestic hot water can be observed. This is the result of new laws and strategies for funding that have been put in place to encourage households to stop using coal and replace it with cleaner energy sources. However, there is a growing concern that gas furnaces are prone to malfunction and can be a threat to occupants through CO (carbon monoxide) generation. To see how a faulty gas furnace with a clogged exhaust may affect a household, a series of multizone and computational fluid dynamics (CFD) simulations were carried out using the CONTAM software and CFD0 editor created by the National Institute of Standards and Technology (NIST). The simulations presented different placements of the furnace and ventilation outlet in an attached garage. The results showed how the placement influenced contaminant migration and occupant exposure to CO. It changed the amount of CO that infiltrated to the attached house and influenced occupant exposure. The results may be used by future users to minimize the risk of CO poisoning by using the proper natural ventilation methods together with optimal placement of the header in the household.
Intestinal Adaptation upon Chemotherapy-Induced Intestinal Injury in Mice Depends on GLP-2 Receptor Activation
Authors: Billeschou Anna , Hunt Jenna Elizabeth , Ghimire Aruna , Holst Jens J. , Kissow Hannelouise
Published: January 2021

Intestinal adaptation is an important response and a natural repair mechanism in acute intestinal injury and is critical for recovery. Glucagon-like peptide 2 (GLP-2) has been demonstrated to enhance mucosal repair following intestinal damage. In this study, we aimed to investigate the role of GLP-2 receptor activation on intestinal protection and adaptation upon chemotherapy-induced intestinal injury. The injury was induced with a single injection of 5-fluorouracil in female GLP-2 receptor knockout (GLP-2R(-/-)) mice and their wild type (WT) littermates. The mice were euthanized in the acute or the recovery phase of the injury; the small intestines were analysed for weight changes, morphology, histology, inflammation, apoptosis and proliferation. In the acute phase, only inflammation was slightly increased in the GLP-2R(-/-) mice compared to WT. In the recovery phase, we observed the natural compensatory response with an increase in small intestinal weight, crypt depth and villus height in WT mice, and this was absent in the GLP-2R(-/-) mice. Both genotypes responded with hyperproliferation. From this, we concluded that GLP-2R signalling does not have a major impact on acute intestinal injury but is pivotal for the adaptive response in the small intestine.
CD73, Tumor Plasticity and Immune Evasion in Solid Cancers
Authors: Yang Haitang , Yao Feng , Davis Paul F. , Tan Swee T. , Hall Sean R. R.
Published: January 2021

Regulatory networks controlling cellular plasticity, important during early development, can re-emerge after tissue injury and premalignant transformation. One such regulatory molecule is the cell surface ectoenzyme ecto-5′-nucleotidase that hydrolyzes the conversion of extracellular adenosine monophosphate to adenosine (eADO). Ecto-5′-nucleotidase (NT5E) or cluster of differentiation 73 (CD73), is an enzyme that is encoded by NT5E in humans. In normal tissue, CD73-mediated generation of eADO has important pleiotropic functions ranging from the promotion of cell growth and survival, to potent immunosuppression mediated through purinergic G protein-coupled adenosine receptors. Importantly, tumors also utilize several mechanisms mediated by CD73 to resist therapeutics and in particular, evade the host immune system, leading to undesired resistance to targeted therapy and immunotherapy. Tumor cell CD73 upregulation is associated with worse clinical outcomes in a variety of cancers. Emerging evidence indicates a link between tumor cell stemness with a limited host anti-tumor immune response. In this review, we provide an overview of a growing body of evidence supporting the pro-tumorigenic role of CD73 and adenosine signaling. We also discuss data that support a link between CD73 expression and tumor plasticity, contributing to dissemination as well as treatment resistance. Collectively, targeting CD73 may represent a novel treatment approach for solid cancers.
Effect of Accumulative Recycling of Aqueous Phase on the Properties of Hydrothermal Degradation of Dry Biomass and Bio-Crude Oil Formation
Authors: Yin Siyuan , Zhang Nianze , Tian Chunyan , Yi Weiming , Yuan Qiaoxia , Fu Peng , Zhang Yuchun , Li Zhiyu
Published: January 2021

For hydrothermal liquefaction of dry biomass to produce liquid fuels, water needs to be added or the aqueous phase products can be recycled. This paper focuses on understanding the relationship between hydrothermal degradation of the dry biomass and oil formation under the condition of accumulative recycling of the aqueous phase. Completely dried corn stalk and deionized water were used for the hydrothermal liquefaction (HTL) experiment. The aqueous products for subsequent recycling were not diluted. It was demonstrated that the recycling of the aqueous can promote the enrichment of organic acids and the conversion of ketones and phenols in the aqueous, improving the yield and quality of Bio-crude oil. After recycling, the yield of Bio-crude oil increased from 20.42% to 24.31% continuously, and the oxygen content decreased from 13.34% to 9.90%. Although the process was accompanied by solid deposition and had a negative impact on the hydrothermal degradation efficiency, the formation of carbon microspheres during the deposition enhanced the utilization of nondegradable solids, while the formation of metal salt particles had a positive impact on oil production. After three rounds of recycling, the solid deposition effect was weakened. At this time, oil production and solids degradation can be promoted simultaneously.
Patterns of Fine-Scale Spatial Genetic Structure and Pollen Dispersal in Giant Sequoia (Sequoiadendron giganteum)
Authors: DeSilva Rainbow , Dodd Richard S.
Published: January 2021

Research Highlights: Patterns of dispersal shape the distribution and temporal development of genetic diversity both within and among populations. In an era of unprecedented environmental change, the maintenance of extant genetic diversity is crucial to population persistence. Background and Objectives: We investigate patterns of pollen dispersal and spatial genetic structure within populations of giant sequoia (Sequoiadendron giganteum). Materials and Methods: The leaf genotypes of established trees from twelve populations were used to estimate the extent of spatial genetic structure within populations, as measured by the Sp statistic. We utilized progeny arrays from five populations to estimate mating parameters, the diversity of the pollen pool, and characteristics of pollen dispersal. Results: Our research indicates that giant sequoia is predominantly outcrossing, but exhibits moderate levels of bi-parental inbreeding (0.155). The diversity of the pollen pool is low, with an average of 7.5 pollen donors per mother tree. As revealed by the Sp-statistic, we find significant genetic structure in ten of twelve populations examined, which indicates the clustering of related individuals at fine spatial scales. Estimates of pollen and gene dispersal indicate predominantly local dispersal, with the majority of pollen dispersal <253 m, and with some populations showing fat-tailed dispersal curves, suggesting potential for long-distance dispersal. Conclusions: The research presented here represent the first detailed examination of the reproductive ecology of giant sequoia, which will provide necessary background information for the conservation of genetic resources in this species. We suggest that restoration planting can mitigate potential diversity loss from many giant sequoia populations.
Design and Implementation of Virtual Security Function Based on Multiple Enclaves
Authors: Wang Juan , Yu Yang , Li Yi , Fan Chengyang , Hao Shirong
Published: January 2021

Network function virtualization (NFV) provides flexible and scalable network function for the emerging platform, such as the cloud computing, edge computing, and IoT platforms, while it faces more security challenges, such as tampering with network policies and leaking sensitive processing states, due to running in a shared open environment and lacking the protection of proprietary hardware. Currently, Intel® Software Guard Extensions (SGX) provides a promising way to build a secure and trusted VNF (virtual network function) by isolating VNF or sensitive data into an enclave. However, directly placing multiple VNFs in a single enclave will lose the scalability advantage of NFV. This paper combines SGX and click technology to design the virtual security function architecture based on multiple enclaves. In our design, the sensitive modules of a VNF are put into different enclaves and communicate by local attestation. The system can freely combine these modules according to user requirements, and increase the scalability of the system while protecting its running state security. In addition, we design a new hot-swapping scheme to enable the system to dynamically modify the configuration function at runtime, so that the original VNFs do not need to stop when the function of VNFs is modified. We implement an IDS (intrusion detection system) based on our architecture to verify the feasibility of our system and evaluate its performance. The results show that the overhead introduced by the system architecture is within an acceptable range.
Knowledge-Graph-Based Drug Repositioning against COVID-19 by Graph Convolutional Network with Attention Mechanism
Authors: Che Mingxuan , Yao Kui , Che Chao , Cao Zhangwei , Kong Fanchen
Published: January 2021

The current global crisis caused by COVID-19 almost halted normal life in most parts of the world. Due to the long development cycle for new drugs, drug repositioning becomes an effective method of screening drugs for COVID-19. To find suitable drugs for COVID-19, we add COVID-19-related information into our medical knowledge graph and utilize a knowledge-graph-based drug repositioning method to screen potential therapeutic drugs for COVID-19. Specific steps are as follows. Firstly, the information about COVID-19 is collected from the latest published literature, and gene targets of COVID-19 are added to the knowledge graph. Then, the information of COVID-19 of the knowledge graph is extracted and a drug–disease interaction prediction model based on Graph Convolutional Network with Attention (Att-GCN) is established. Att-GCN is used to extract features from the knowledge graph and the prediction matrix reconstructed through matrix operation. We evaluate the model by predicting drugs for both ordinary diseases and COVID-19. The model can achieve area under curve (AUC) of 0.954 and area under the precise recall area curve (AUPR) of 0.851 for ordinary diseases. On the drug repositioning experiment for COVID-19, five drugs predicted by the models have proved effective in clinical treatment. The experimental results confirm that the model can predict drug–disease interaction effectively for both normal diseases and COVID-19.
Influence of a Standing Wave Flow-Field on the Dynamics of a Spray Diffusion Flame
Authors: Greenberg J. Barry , Katoshevski David
Published: January 2021

A theoretical investigation of the influence of a standing wave flow-field on the dynamics of a laminar two-dimensional spray diffusion flame is presented for the first time. The mathematical analysis permits mild slip between the droplets and their host surroundings. For the liquid phase, the use of a small Stokes number as the perturbation parameater enables a solution of the governing equations to be developed. Influence of the standing wave flow-field on droplet grouping is described by a specially constructed modification of the vaporization Damkohler number. Instantaneous flame front shapes are found via a solution for the usual Schwab–Zeldovitch parameter. Numerical results obtained from the analytical solution uncover the strong bearing that droplet grouping, induced by the standing wave flow-field, can have on flame height, shape, and type (over- or under-ventilated) and on the existence of multiple flame fronts.
Special Issue “Molecular Basis of Inherited Diseases in Companion Animals”
Authors: Friedenberg Steven G. , Bannasch Danika L.
Published: January 2021

The study of inherited diseases in companion animals has exploded over the past 15 years since the publication of the first dog genome in 2005 [...]
The GeriPACT Initiative to Prevent All-Cause 30-Day Readmission in High Risk Elderly
Authors: Powers James S. , Abraham Lovely , Parker Ralph , Azubike Nkechi , Habermann Ralf
Published: January 2021

Background: Suboptimal care transitions increases the risk of adverse events resulting from poor care coordination among providers and healthcare facilities. The National Transition of Care Coalition recommends shifting the discharge paradigm from discharge from the hospital, to transfer with continuous management. The patient centered medical home is a promising model, which improves care coordination and may reduce hospital readmissions. Methods: This is a quality improvement report, the geriatric patient-aligned care team (GeriPACT) at Tennessee Valley Healthcare System (TVHS) participated in ongoing quality improvement (Plan, Do, Study, Act (PDSA)) cycles during teamlet meetings. Post home discharge follow-up for GeriPACT patients was provided by proactive telehealth communication by the Registered Nurse (RN) care manager and nurse practitioner. Periodic operations data obtained from the Data and Statistical Services (DSS) coordinator informed the PDSA cycles and teamlet meetings. Results: at baseline (July 2018–June 2019) the 30-day all-cause readmission for GeriPACT was 21%. From July to December 2019, 30-day all-cause readmissions were 13%. From January to June 2020, 30-day all-cause readmissions were 15%. Conclusion: PDSA cycles with sharing of operations data during GeriPACT teamlet meetings and fostering a shared responsibility for managing high-risk patients contributes to improved outcomes in 30-day all-cause readmissions.
A Decrease in Psycho-Emotional Health in Middle-Aged Russian Women Associated with Their Lifestyle
Authors: Saporovskaia Maria V. , Kryukova Tatiana L. , Voronina Maria E. , Tikhomirova Elena V. , Samokhvalova Anna G. , Khazova Svetlana A.
Published: January 2021

(1) Background: The present study is aimed to determine the predicting role of objective (lifestyle) and subjective factors of middle-aged women’s psycho-emotional health such as their relations with parents, attachment and separation types. Women who are overloaded with professional and family roles have high stress level, their indicators of psychological well-being and emotional level decrease when they have to give everyday care to their elderly parents. (2) Methods: The research sample has two empirical groups. Sample of Study 1 includes middle-aged women (n = 61) aged 38–56 (M = 48.1, SD = 3.5); sample of Study 2 includes middle-aged women (n = 85) aged 33–52 (M = 40.6, SD = 3.1): married (70.5%) and divorced (29.5%), having children of 14–28 years old; giving everyday care to elderly parents for more than 1.5 yrs. Some live separately (62.3 %), or have to cohabitate with parents (37.7%). All women evaluate their life situation as difficult and manifest signs of high psycho-emotional stress. We used methods adapted for the Russian-speaking sample: getting socio-demographic information, an interview; The scales of psychological well-being; Attachment style and Interpersonal Guilt Questionnaires (study 1); Psychological Separation Inventory, Purpose-in-Life Test, projective methods (study 2), mathematical statistics. (3) Results: A number of factors and indicators of women’s psycho-emotional health decrease in the situation of role overload have been identified. Among the factors there are four main types of women’s relationships with parents: Anxious closeness; Ambivalence of feelings; Secondary relationship with parents; Alienation, predicting of psycho-emotional health that are reducing or enhancing their personal resources. Besides, a type of separation of an adult woman from her mother predicts her level of well-being. (4) Conclusions: The study confirms that middle-aged Russian women’s psycho-emotional health depends on contextual factors (difficult role-overloaded lifestyle) and factors integrating women‘s relations towards parents, attachment, guilt and separation. Types of middle-aged women’s relationships with parents contribute to their psychoemotional health in a different way.
Weather Variability and COVID-19 Transmission: A Review of Recent Research
Authors: McClymont Hannah , Hu Wenbiao
Published: January 2021

Weather and climate play a significant role in infectious disease transmission, through changes to transmission dynamics, host susceptibility and virus survival in the environment. Exploring the association of weather variables and COVID-19 transmission is vital in understanding the potential for seasonality and future outbreaks and developing early warning systems. Previous research examined the effects of weather on COVID-19, but the findings appeared inconsistent. This review aims to summarize the currently available literature on the association between weather and COVID-19 incidence and provide possible suggestions for developing weather-based early warning system for COVID-19 transmission. Studies eligible for inclusion used ecological methods to evaluate associations between weather (i.e., temperature, humidity, wind speed and rainfall) and COVID-19 transmission. The review showed that temperature was reported as significant in the greatest number of studies, with COVID-19 incidence increasing as temperature decreased and the highest incidence reported in the temperature range of 0–17 °C. Humidity was also significantly associated with COVID-19 incidence, though the reported results were mixed, with studies reporting positive and negative correlation. A significant interaction between humidity and temperature was also reported. Wind speed and rainfall results were not consistent across studies. Weather variables including temperature and humidity can contribute to increased transmission of COVID-19, particularly in winter conditions through increased host susceptibility and viability of the virus. While there is less indication of an association with wind speed and rainfall, these may contribute to behavioral changes that decrease exposure and risk of infection. Understanding the implications of associations with weather variables and seasonal variations for monitoring and control of future outbreaks is essential for early warning systems.
Adsorption of Methylene Blue Dye by Calix[6]Arene-Modified Lead Sulphide (Pbs): Optimisation Using Response Surface Methodology
Authors: Rosly Nor Zida , Abdullah Abdul Halim , Ahmad Kamarudin Mazliana , Ashari Siti Efliza , Alang Ahmad Shahrul Ainliah
Published: January 2021

Lead sulphide (PbS) modified with calix[6]arene was synthesised as an alternative and regenerative adsorbent for the adsorption of methylene blue (MB) dye. The prepared calix[6]arene-modified PbS was characterised via Fourier-transform infrared spectroscopy, field emission scanning electron microscopy, and energy-dispersive X-ray spectroscopy. The response surface methodology (RSM) based on the central composite design (CCD) was employed to identify the most significant factors, such as the initial concentration, adsorbent dosage, pH, and temperature, and to optimise the effects of the factors on the adsorptive efficiency as its response. The optimised initial concentration, adsorbent dosage, pH, and temperature were 20.00 mg/L initial concentration, 44.00 mg calix[6]arene-modified PbS, pH 6, and a temperature of 31.00 °C. A good correlation between the values and well-fitted model was observed. The adsorption performance was evaluated based on the percentage removal of MB dye from the water system. The adsorption isotherm best fit the Langmuir isotherm model, and the adsorption rate was followed by a pseudo-second-order kinetic model, a single layer chemical adsorption with a maximum adsorption capacity (qmax) of 5.495 mg/g.
Fate of Emerging Contaminants in High-Rate Activated Sludge Systems
Authors: Koumaki Elena , Noutsopoulos Constantinos , Mamais Daniel , Fragkiskatos Gerasimos , Andreadakis Andreas
Published: January 2021

High-rate activated sludge (HRAS) systems are designed to shift the energy-intensive processes to energy-saving and sustainable technologies for wastewater treatment. The high food-to-microorganism (F/M) ratios and low solid retention times (SRTs) and hydraulic retention times (HRTs) applied in HRAS systems result in the maximization of organic matter diversion to the sludge which can produce large amounts of biogas during anaerobic digestion, thus moving toward energy-neutral (or positive) treatment processes. However, in addition to the energy optimization, the removal of emerging contaminants (ECs) is the new challenge in wastewater treatment. In the context of this study, the removal efficiencies and the fates of selected ECs (three endocrine disruptors (endocrine disrupting chemicals (EDCs))—nonylphenol, bisphenol A and triclosan, and four pharmaceuticals (PhACs)—ibuprofen, naproxen, diclofenac and ketoprofen) in HRAS systems have been studied. According to the results, EDCs occurred in raw wastewater and secondary sludge at higher concentrations compared to PhACs. In HRAS operating schemes, all compounds were poorly (<40%) to moderately (<60%) removed. Regarding removal mechanisms, biotransformation was found to be the dominant process for PhACs, while for EDCs sorption onto sludge is the most significant removal mechanism affecting their fates and their presence in excess sludge.
Effects of Strength vs. Plyometric Training Programs on Vertical Jumping, Linear Sprint and Change of Direction Speed Performance in Female Soccer Players: A Systematic Review and Meta-Analysis
Authors: Pardos-Mainer Elena , Lozano Demetrio , Torrontegui-Duarte Marcelino , Cartón-Llorente Antonio , Roso-Moliner Alberto
Published: January 2021

The main purpose of this systematic review and meta-analysis was to compare the effects of strength training (ST) and plyometric training (PT) on vertical jump, linear sprint and change of direction (COD) performance in female soccer players. A systematic search of the PubMed, Web of Science, Google Scholar and SportDiscus databases revealed 12 studies satisfying the inclusion criteria. The inverse-variance random-effects model for meta-analyses was used. Effect sizes (ES) were represented by the standardized mean difference and presented alongside 95% confidence intervals (CI). The magnitude of the main effect was small to moderate (vertical jump (ES 0.53 (95% CI—0.11, 0.95), Z = 2.47 (p = 0.01); linear sprint (ES −0.66 (95% CI—2.03, −0.21), Z = 2.20 (p = 0.03); COD (ES −0.36 (95% CI—0.68, −0.03), Z = 2.17 (p = 0.03)). Subgroup analyses were performed (i.e., ST and PT duration, frequency, session duration and total number of sessions), revealing no significant subgroup differences (p = 0.12–0.88). In conclusion, PT provides better benefits than ST to improve vertical jump, linear sprint and COD performance in female soccer players. However, significant limitations in the current literature prevent assured PT and ST prescription recommendations being made.
Increasing Registered Nurse Hours Per Resident Day for Improved Nursing Home Residents’ Outcomes Using a Longitudinal Study
Authors: Shin Juh Hyun , Renaut Rosemary Anne , Reiser Mark , Lee Ji Yeon , Tang Ty Yi
Published: January 2021

The purpose of this study was to estimate how much resident outcomes can improve with an increase in hours per resident day (HPRD) of registered nurses (RNs) staffing. Nursing home (NH) staff in Korea have serious problems with inappropriate nurse staffing standards and poor working conditions, which lead to poor quality of care for NH residents. This study used a longitudinal survey design. A quota sampling was used with a total of several repeated survey measurement from 2017 to 2020 (n = 74). The independent variable was the amount of nurse staffing HPRD and the outcome variable was the compiled outcome of 15 quality-of-care indicators. Data were directly collected from all participating NHs. A longitudinal, multilevel model was used for analysis. An increase of one unit of RN HPRD (60 min) corresponded to a decrease of about 10.5% of residents with deteriorated quality of care outcomes. This study emphasized that increasing RN HPRD decreased residents’ deteriorated outcomes in NHs. This suggests that professional RNs must be secured to an appropriate level to improve the quality of care for NH residents.
Dietary Intake and Elevated C-Reactive Protein Levels in US Military Veterans
Authors: Ansley Stephanie D. , Howard Jeffrey T.
Published: January 2021

Elevated inflammatory markers, such as high sensitivity C-reactive protein (hs-CRP), have been associated with the pathogenesis of cardiovascular disease (CVD)-related diseases. However, limited studies have evaluated the potential association between dietary consumption and hs-CRP levels in a large, nationally representative sample, and fewer have investigated their role in ethnic and racial minority military populations. The goal of this study is to explore the relationship between dietary intake, health behaviors, and hs-CRP in individuals with prior military status and whether these associations differ by race/ethnicity. A complex, multistage, probability sample design was used from the National Health and Nutrition Examination (NHANES) 2015–2018 waves. Our results indicate that previously deployed military service members had a higher prevalence of clinically elevated hs-CRP levels than civilians. Differences in hs-CRP among deployed veterans and civilians remained even after multivariable adjustment. Individuals classified as overweight and obese demonstrated clinically elevated hs-CRP levels compared with those with a normal body mass index (BMI). Dietary factors did not attenuate the association between changes in hs-CRP levels and veteran status. These findings suggest the need for further investigation into how military-specific stressors contribute to unfavorable health outcomes for the military population.
Evaluation of Winter Footwear: Comparison of Test Methods to Determine Footwear Slip Resistance on Ice Surfaces
Authors: Roshan Fekr Atena , Li Yue , Gauvin Chantal , Wong Gordon , Cheng Wayne , Fernie Geoff , Dutta Tilak
Published: January 2021

The use of slip-resistant winter footwear is crucial for the prevention of slips and falls on ice and snow. The main objective of this paper is to evaluate a mechanical testing method to determine footwear slip resistance on wet and dry ice surfaces and to compare it with the human-centred test method introduced by researchers at KITE (Knowledge, Innovation, Talent, Everywhere)-Toronto Rehabilitation Institute-University Health Network. Phase 1 of this study assessed the repeatability and reproducibility of the mechanical method by evaluating ten different occupational winter boots using two SATRA Slip resistance testers (STM 603, SATRA Technology Centre, Kettering, UK). One tester is located in Toronto and one in Montreal. These boots were chosen based on the needs of the IRSST (Institut de Recherche Robert-Sauvé en Santé et en Sécurité du Travail, Montréal, Quebec, Canada), who were primarily interested in providing safe winter footwear for police, firefighters and municipal workers. In Phase 2, the results of the human-centred test approach were compared with the mechanical results. In Phase 3, two of these boots with conflicting results from the previous phases were tested using a second human-centred method. In Phase 1, the mechanical testing results obtained in the two labs showed a high linear correlation (>0.94) and good agreement on both ice surfaces; however, they revealed a bias (~0.06) between the two labs on the dry ice condition. The mechanical and human-centred tests (phase 2) were found to be better correlated in the wet ice condition (R = 0.95) compared to the dry ice condition (R = 0.34). Finally, the rating of the footwear slip resistance based on the number of slips counted in phase 3 was consistent with the rating by the human-centred test method (phase 2), but not the mechanical method (phase 1). The findings of this study provide a better understanding of the limitations of the SATRA ice tray for measuring footwear slip resistance and demonstrate that the mechanical method must be further refined to make it more comparable to the human-centred methods to achieve better agreement with real-world performance.
Measuring Temporal Instability of Momentary Affect, Subjective Health and Symptom Burden Depending on Environmental Parameters—An Ambulatory Assessment Study in Persons with Hay Fever
Authors: Rostalski Tim , Schmidt Silke , Muehlan Holger
Published: January 2021

Our ambulatory assessment study explores the impact of the weather on the mental well-being of people with increased susceptibility. Participants with hay fever (n = 28) were assessed three times a day over a period of two weeks. Self-reported assessments covered different indicators of mental well-being, including momentary affect, subjective health as well as symptom burden. Based on tracked time stamps and location information, the data was matched with concurrent observation data from weather stations. We applied multilevel analysis to identify the main effects of selected environmental parameters (temperature, precipitation, wind power, sunshine duration and relative humidity) on all indicators of subjective well-being. Results confirm the main effects of sunshine duration, relative humidity and temperature on momentary affect as well as of sunshine duration, relative humidity and precipitation on subjective health and symptom burden. However, influences of environmental parameters on momentary affect were quite small and do not differ from effects documented in previous research in healthy samples with non-increased susceptibility.
Hyperbaric Oxygen Therapy and A-PRF Pre-Treated Implants in Severe Periodontitis: A Case Report
Authors: Giacon Tommaso Antonio , Giancola Franco , Paganini Matteo , Tiengo Cesare , Camporesi Enrico M. , Bosco Gerardo
Published: January 2021

Implantation is currently the best option for tooth replacement in periodontitis. Some major contraindications for the immediate implant are acute periodontitis and active infection. We present the case of a 51-year-old female patient with the highest grade and stage periodontitis treated with advanced platelet-rich fibrin-enriched zirconia implants and with hyperbaric oxygen therapy (HBOT). In particular, HBOT before and after implantation promoted bone regeneration and implant integration, also providing an antiseptic effect. After six months, the implants were well established and fully healed from periodontal disease within 14 months. Further research could confirm a new indication for HBOT in treating periodontitis and dental implantation.
Reduced Graphene Oxides Modulate the Expression of Cell Receptors and Voltage-Dependent Ion Channel Genes of Glioblastoma Multiforme
Authors: Szczepaniak Jaroslaw , Jagiello Joanna , Wierzbicki Mateusz , Nowak Dorota , Sobczyk-Guzenda Anna , Sosnowska Malwina , Jaworski Slawomir , Daniluk Karolina , Szmidt Maciej , Witkowska-Pilaszewicz Olga , Strojny-Cieslak Barbara , Grodzik Marta
Published: January 2021

The development of nanotechnology based on graphene and its derivatives has aroused great scientific interest because of their unusual properties. Graphene (GN) and its derivatives, such as reduced graphene oxide (rGO), exhibit antitumor effects on glioblastoma multiforme (GBM) cells in vitro. The antitumor activity of rGO with different contents of oxygen-containing functional groups and GN was compared. Using FTIR (fourier transform infrared) analysis, the content of individual functional groups (GN/exfoliation (ExF), rGO/thermal (Term), rGO/ammonium thiosulphate (ATS), and rGO/ thiourea dioxide (TUD)) was determined. Cell membrane damage, as well as changes in the cell membrane potential, was analyzed. Additionally, the gene expression of voltage-dependent ion channels (clcn3, clcn6, cacna1b, cacna1d, nalcn, kcne4, kcnj10, and kcnb1) and extracellular receptors was determined. A reduction in the potential of the U87 glioma cell membrane was observed after treatment with rGO/ATS and rGO/TUD flakes. Moreover, it was also demonstrated that major changes in the expression of voltage-dependent ion channel genes were observed in clcn3, nalcn, and kcne4 after treatment with rGO/ATS and rGO/TUD flakes. Furthermore, the GN/ExF, rGO/ATS, and rGO/TUD flakes significantly reduced the expression of extracellular receptors (uPar, CD105) in U87 glioblastoma cells. In conclusion, the cytotoxic mechanism of rGO flakes may depend on the presence and types of oxygen-containing functional groups, which are more abundant in rGO compared to GN.
Inhibition of Stress-Induced Viral Promoters by a Bovine Herpesvirus 1 Non-Coding RNA and the Cellular Transcription Factor, β-Catenin
Authors: Zhao Jing , Wijesekera Nishani , Jones Clinton
Published: January 2021

The ability to establish, maintain, and reactivate from latency in sensory neurons within trigeminal ganglia (TG) is crucial for bovine herpesvirus 1 (BoHV-1) transmission. In contrast to lytic infection, the only viral gene abundantly expressed during latency is the latency-related (LR) gene. The synthetic corticosteroid dexamethasone consistently induces reactivation from latency, in part because the glucocorticoid receptor (GR) transactivates viral promoters that drive expression of key viral transcriptional regulator proteins (bICP0 and bICP4). Within hours after dexamethasone treatment of latently infected calves, LR gene products and β-catenin are not readily detected in TG neurons. Hence, we hypothesized that LR gene products and/or β-catenin restrict GR-mediated transcriptional activation. A plasmid expressing LR RNA sequences that span open reading frame 2 (ORF2-Stop) inhibited GR-mediated transactivation of the BoHV-1 immediate early transcription unit 1 (IEtu1) and mouse mammary tumor virus (MMTV) promoter activity in mouse neuroblastoma cells (Neuro-2A). ORF2-Stop also reduced productive infection and GR steady-state protein levels in transfected Neuro-2A cells. Additional studies revealed that the constitutively active β-catenin mutant reduced the transactivation of the IEtu1 promoter by GR and dexamethasone. Collectively, these studies suggest ORF2 RNA sequences and Wnt/β-catenin signaling pathway actively promote maintenance of latency, in part, by impairing GR-mediated gene expression.
Endothelial Cells as Tools to Model Tissue Microenvironment in Hypoxia-Dependent Pathologies
Authors: Majewska Aleksandra , Wilkus Kinga , Brodaczewska Klaudia , Kieda Claudine
Published: January 2021

Endothelial cells (ECs) lining the blood vessels are important players in many biological phenomena but are crucial in hypoxia-dependent diseases where their deregulation contributes to pathology. On the other hand, processes mediated by ECs, such as angiogenesis, vessel permeability, interactions with cells and factors circulating in the blood, maintain homeostasis of the organism. Understanding the diversity and heterogeneity of ECs in different tissues and during various biological processes is crucial in biomedical research to properly develop our knowledge on many diseases, including cancer. Here, we review the most important aspects related to ECs’ heterogeneity and list the available in vitro tools to study different angiogenesis-related pathologies. We focus on the relationship between functions of ECs and their organo-specificity but also point to how the microenvironment, mainly hypoxia, shapes their activity. We believe that taking into account the specific features of ECs that are relevant to the object of the study (organ or disease state), especially in a simplified in vitro setting, is important to truly depict the biology of endothelium and its consequences. This is possible in many instances with the use of proper in vitro tools as alternative methods to animal testing.