bluuebunny/crossref_metadata_2025 huggingface dataset:
The Novel LncRNA WASH5P Inhibits Colorectal Cancer Carcinogenesis via Targeting AKT Signaling Pathway
Authors: Wei Hongyun , Mao Tao , Zhang Qian , Ren Keyu , Qi Xingsi , Zhang Yunmei , Cao Bin , Jin Yanchun , Tian Zibin , Ren Linlin
Published: July 2022

Emerging evidence has shown that long non-coding RNAs (lncRNAs) play an important role in colorectal cancer (CRC) carcinogenesis, so more specific mechanisms of key lncRNAs in CRC initiation and development are needed. Here, we evaluated the expression profiles of lncRNAs in CRC tissues and identified a novel lncRNA generated from the pseudogene Wiskott-Aldrich syndrome protein (WASP) family homolog 5, termed lncRNA WASH5P. However, the role and potential molecular mechanism of this novel lncRNA in diseases, including CRC carcinogenesis, is unknown. Our present study found that WASH5P was significantly downregulated in CRC cell lines and tissues compared with normal controls. The ectopic expression of WASH5P in CRC cells could significantly inhibit CRC cell proliferation, invasion, and migration. In addition, WASH5P could increase the expression of E-cadherin and decrease Vimentin expression. WASH5P-overexpressing CRC cells developed tumors more slowly in different mouse models. Meanwhile, the overexpression of WASH5P could significantly inhibit AKT activation via suppressing AKT phosphorylation. The treatment of PI3K/AKT (phosphatidlinositol 3-kinase /protein kinase B) signaling agonist 740Y-P rescued WASH5P-reduced AKT phosphorylation and abolished the inhibitory effects of WASH5P on cell viability, migration, and invasion. Moreover, 740Y-P restored the WASH5P-induced downregulation of p-AKT and vimentin and the upregulation of E-cadherin via Western blot. In summary, our findings suggested that the novel lncRNA WASH5P might be a potential candidate biomarker and therapeutic target that could inhibit CRC by repressing the AKT signaling pathway.
Overexpression of OAS1 Is Correlated With Poor Prognosis in Pancreatic Cancer
Authors: Lu Lingling , Wang Huaxiang , Fang Jian , Zheng Jiaolong , Liu Bang , Xia Lei , Li Dongliang
Published: July 2022

Background

OAS1 expression in pancreatic cancer has been confirmed by many studies. However, the prognostic value and mechanism of OAS1 in pancreatic cancer have not been analyzed.

Methods

The RNA-seq in pancreatic cancer were obtained by UCSC XENA and GEO database. In addition, immunohistochemical validation and analysis were performed using samples from the 900th hospital. The prognosis of OAS1 was evaluated by timeROC package, Cox regression analysis, and Kaplan-Meier survival curves. Then, the main functional and biological signaling pathways enrichment and its relationship with the abundance of immune cells were analyzed by bioinformatics.

Results

OAS1 was highly expressed in pancreatic cancer compared with normal pancreatic tissue. High OAS1 expression was associated with poor overall survival (p<0.05). The OAS1 was significantly correlated to TNM staging (p=0.014). The timeROC analysis showed that the AUC of OAS1 was 0.734 for 3-year OS. In addition, the expression of OAS1 was significantly correlated with the abundance of a variety of immune markers. GSEA showed that enhanced signaling pathways associated with OAS1 include Apoptosis, Notch signaling pathway, and P53 signaling pathway.

Conclusions

OAS1 is a valuable prognostic factor in pancreatic cancer. Moreover, it may be a potential immunotherapeutic target.
The Rise of Functional Tic-Like Behaviors: What Do the COVID-19 Pandemic and Social Media Have to Do With It? A Narrative Review
Authors: Martindale Jaclyn M. , Mink Jonathan W.
Published: July 2022

Background

There has been a rise in explosive onset of tic-like behaviors during the COVID-19 pandemic. Historically, this is an uncommon phenomenology of functional movement disorders across all ages. Both the psychological burden of the pandemic and social media usage have been implicated in the rise of these tic-like behaviors.

Methods

This paper provides a narrative review of the literature on chronic tic disorders, functional tics, and mass functional illness with particular focus on the key distinguishing features, role of social media, and the role of COVID-19.

Results

The COVID-19 pandemic has profoundly affected the mental health of many individuals, including children, adolescents, and their caregivers. Implementation of lockdowns, lifestyle disruptions, school closures, and social distancing have driven a surge in social media and digital technology use. The combination of predisposing factors, the psychological burden of the COVID-19 pandemic, and social media are implicated in the rise and spread of tic-like behaviors; which may represent a modern-day form of mass functional illness. While many of the features overlap with functional tics, there are emerging distinctive features that are important to recognize. A more encompassing term, Functional Tic-Like Behaviors, is used to better reflect multiple contributing factors.

Conclusion

Knowledge of these differences is essential to mitigate downstream health effects and poor outcomes.
Case Report: Primary Leptomeningeal Medulloblastoma in a Child: Clinical Case Report and Literature Review
Authors: Morgacheva Daria , Daks Alexandra , Smirnova Anna , Kim Aleksandr , Ryzhkova Daria , Mitrofanova Lubov , Staliarova Alena , Omelina Evgeniya , Pindyurin Alexey , Fedorova Olga , Shuvalov Oleg , Petukhov Alexey , Dinikina Yulia
Published: July 2022

Medulloblastoma is one of the most common pediatric central nervous system malignancies worldwide, and it is characterized by frequent leptomeningeal metastasizing. We report a rare case of primary leptomeningeal medulloblastoma of an 11-year-old Caucasian girl with a long-term disease history, non-specific clinical course, and challenges in the diagnosis verification. To date, 4 cases of pediatric primary leptomeningeal medulloblastoma are reported, and all of them are associated with unfavorable outcomes. The approaches of neuroimaging and diagnosis verification are analyzed in the article to provide opportunities for effective diagnosis of this disease in clinical practice. The reported clinical case of the primary leptomeningeal medulloblastoma is characterized by MR images with non-specific changes in the brain and spinal cord and by 18FDG-PET/CT images with diffuse heterogeneous hyperfixation of the radiopharmaceutical along the whole spinal cord. The immunohistochemistry and next-generation sequencing analyses of tumor samples were performed for comprehensive characterization of the reported clinical case.
Functional Training Focused on Motor Development Enhances Gross Motor, Physical Fitness, and Sensory Integration in 5–6-Year-Old Healthy Chinese Children
Authors: Fu Tao , Zhang Diruo , Wang Wei , Geng Hui , Lv Yao , Shen Ruiheng , Bu Te
Published: July 2022

Objective

Physical inactivity and sensory integration dysfunction are public health concerns among Chinese preschool children. The purpose of this study was to determine the efficacy of a novel functional training program focused on motor development for healthy children aged 5 to 6 years.

Methods

A total of 101 healthy children aged 5 to 6 years in Tianjin were randomly assigned to the experimental group (N = 51), which received 12-week functional training featuring essential motor skills, whilst the control group (N = 50) continued with their kindergarten-based physical education curriculum. Test of Gross Motor Development-2, national physical fitness measurement, and sensory integration were evaluated before and after the intervention. Children's height, body weight, and the corresponding pre-intervention test scores were utilized as covariates to compare the post-intervention outcomes between the groups.

Results

After the intervention, the experimental group scored considerably higher (P < 0.01) on the locomotor composite score, object control composite score, and overall gross motor score than the control group; the experimental group scored higher (P < 0.05) on the run, gallop, leap, stationary dribble, kick, striking a stationary ball, overhand throw, and underhand roll motor skill tests than the control group; the experimental group performed considerably better (P < 0.01) on the balance beam walking test and sit-and-reach test than the control group; and, the experimental group performed considerably better (P < 0.01) on the vestibular function, tactile defensiveness, and proprioception than the control group.

Conclusion

A 12-week functional training focused on motor development effectively enhanced gross motor, physical fitness, and sensory integration in 5–6-year-old healthy Chinese children.
Effect of Tanshinone IIA on Gut Microbiome in Diabetes-Induced Cognitive Impairment
Authors: Zheng Yanfang , Zhou Xian , Wang Chenxiang , Zhang Jialin , Chang Dennis , Liu Wenjing , Zhu MingXing , Zhuang Shuting , Shi Hong , Wang Xiaoning , Chen Yong , Cheng Zaixing , Lin Yanxiang , Nan Lihong , Sun Yibin , Min Li , Liu Jin , Chen Jianyu , Zhang Jieping , Huang Mingqing
Published: July 2022

Diabetes-induced cognitive impairment (DCI) presents a major public health risk among the aging population. Previous clinical attempts on known therapeutic targets for DCI, such as depleted insulin secretion, insulin resistance, and hyperglycaemia have delivered poor patient outcomes. However, recent evidence has demonstrated that the gut microbiome plays an important role in DCI by modulating cognitive function through the gut–brain crosstalk. The bioactive compound tanshinone IIA (TAN) has shown to improve cognitive and memory function in diabetes mellitus models, though the pharmacological actions are not fully understood. This study aims to investigate the effect and underlying mechanism of TAN in attenuating DCI in relation to regulating the gut microbiome. Metagenomic sequencing analyses were performed on a group of control rats, rats with diabetes induced by a high-fat/high-glucose diet (HFD) and streptozotocin (STZ) (model group) and TAN-treated diabetic rats (TAN group). Cognitive and memory function were assessed by the Morris water maze test, histopathological assessment of brain tissues, and immunoblotting of neurological biomarkers. The fasting blood glucose (FBG) level was monitored throughout the experiments. The levels of serum lipopolysaccharide (LPS) and tumor necrosis factor-α (TNF-α) were measured by enzyme-linked immunoassays to reflect the circulatory inflammation level. The morphology of the colon barrier was observed by histopathological staining. Our study confirmed that TAN reduced the FBG level and improved the cognitive and memory function against HFD- and STZ-induced diabetes. TAN protected the endothelial tight junction in the hippocampus and colon, regulated neuronal biomarkers, and lowered the serum levels of LPS and TNF-α. TAN corrected the reduced abundance of Bacteroidetes in diabetic rats. At the species level, TAN regulated the abundance of B. dorei, Lachnoclostridium sp. YL32 and Clostridiodes difficile. TAN modulated the lipid metabolism and biosynthesis of fatty acids in related pathways as the main functional components. TAN significantly restored the reduced levels of isobutyric acid and butyric acid. Our results supported the use of TAN as a promising therapeutic agent for DCI, in which the underlying mechanism may be associated with gut microbiome regulation.
Metabolomics Profiles Associated with the Treatment of Zuojin Pill on Patients with Chronic Nonatrophic Gastritis
Authors: Ma Xiao , Xie Shuying , Wang Ruilin , Wang Zhongxia , Jing Manyi , Li Haotian , Wei Shizhang , Liu Honghong , Li Jianyu , He Qingyong , Zhao Yanling
Published: July 2022

Objective: Chronic nonatrophic gastritis (CNG) is the most common digestive disease. In China, Zuojin pill (ZJP) is considered an effective medicine formula for CNG. However, its efficacy and mechanism have never been explored. In order to understand how and why ZJP demonstrates therapeutic effect on CNG, a clinical trial was conducted. Metabolomics was used to explore its deep mechanism.

Methods: A total of 14 patients with CNG were recruited from October 2020 to March 2021 (ChiCTR2000040549). The endoscopy and histopathological changes were evaluated as efficacy. Serum samples were prepared and detected by performing widely targeted metabolome using UPLC. Multivariate statistical analysis was conducted to identify potential differential metabolites and signaling pathways. Last, the signal-related inflammatory factors containing COX-2, IL-4, and IL-17 were confirmed via immunohistochemical staining and enzyme-linked immunosorbent assay.

Results: ZJP was able to alleviate several indexes of mucosal injury under endoscopy and histology. Erosion and bile reflux, but not red plaques and hemorrhage, were downregulated by ZJP. In addition, it could remarkably alleviate active chronic inflammation. A total of 14 potential metabolites, namely, hypoxanthine, adipic acid, D-ribono-1,4-lactone, L-sepiapterin, imidazoleacetic acid, sebacate, ADP-ribose, 4-hydroxybenzyl alcohol, 11,12-EET, 15-OxoETE, 12-OxoETE, (±)8-HETE, glycyrrhizinate, and DL-aminopimelic acid, were discriminated by metabolomics. Moreover, certain amino acid metabolism got significance during the disease progress and treatment. The related inflammatory factors including COX-2, IL-4, and IL-17 were inhibited by ZJP in both mucosa and serum.

Conclusion: All these results indicated that ZJP partially acts as an inflammatory suppressor to regulate comprehensive metabolism disorders. This might be an important mechanism of ZJP in the treatment of CNG.
Genetic Variants in Double-Strand Break Repair Pathway Genes to Predict Platinum-Based Chemotherapy Prognosis in Patients With Lung Cancer
Authors: Liu Jun-Yan , Zou Ting , Yin Ji-Ye , Wang Zhan , Liu Chong , Huang Han-Xue , Ding Fei-Xiang , Lei Meng-Rong , Wang Ying , Liu Min , Liu Zhao-Qian , Tan Li-Ming , Chen Juan
Published: July 2022

Objective: The purpose of this study was to investigate the associations of genetic variants in double-strand break (DSB) repair pathway genes with prognosis in patients with lung cancer treated with platinum-based chemotherapy.

Methods: Three hundred ninety-nine patients with lung cancer who received platinum-based chemotherapy for at least two cycles were included in this study. A total of 35 single nucleotide polymorphisms (SNPs) in DSB repair, base excision repair (BER), and nucleotide excision repair (NER) repair pathway genes were genotyped, and were used to evaluate the overall survival (OS) and the progression-free survival (PFS) of patients who received platinum-based chemotherapy using Cox proportional hazard models.

Results: The PFS of patients who carried the MAD2L2 rs746218 GG genotype was shorter than that in patients with the AG or AA genotypes (recessive model: p = 0.039, OR = 5.31, 95% CI = 1.09–25.93). Patients with the TT or GT genotypes of TNFRSF1A rs4149570 had shorter OS times than those with the GG genotype (dominant model: p = 0.030, OR = 0.57, 95% CI = 0.34–0.95). We also investigated the influence of age, gender, histology, smoking, stage, and metastasis in association between SNPs and OS or PFS in patients with lung cancer. DNA repair gene SNPs were significantly associated with PFS and OS in the subgroup analyses.

Conclusion: Our study showed that variants in MAD2L2 rs746218 and TNFRSF1A rs4149570 were associated with shorter PFS or OS in patients with lung cancer who received platinum-based chemotherapy. These variants may be novel biomarkers for the prediction of prognosis of patients with lung cancer who receive platinum-based chemotherapy.
The Outcomes of Sodium-Glucose Co-transporter 2 Inhibitors (SGLT2I) on Diabetes-Associated Neuropathy: A Systematic Review and meta-Analysis
Authors: Kandeel Mahmoud
Published: July 2022

Diabetes mellitus (DM) is one of the leading causes of morbidity and mortality worldwide. DM patients with diabetic neuropathy (DN) usually present with distal pain, sensorimotor polyneuropathy, postural hypotension, or erectile dysfunction. They also may present with other nerve pathologies such as inflammatory neuropathies and carpal tunnel syndrome. We conducted a systematic review and meta-analysis to assess the benefits of using sodium–glucose co-transporter-2 inhibitors (SGLT2Is) to manage DN. An extensive systematic literature review was conducted to include all articles published up to 24 February 2022. All clinical studies included patients with DM and reported the outcomes of SGLT2I on diabetes-associated neuropathy. Six studies were identified for meta-analysis, including a total of 5312 diabetic patients. The average age of the included patients ranged from 41 to 74 years and 34–73 years in the SGLT2I treatment and control groups, respectively. SGLT2I moderately improved the manifestations of diabetic peripheral neuropathy events and nerve conduction velocity. Furthermore, the SGLT2I treatment group had a statistically significant higher mean heart-to-mediastinum ratio (MD 0.41; 95% 0.17, 0.64; p = 0.0006). However, the mean heart rates (MD −4.51; 95% −10.05, 1.04; p = 0.11) and wash out rates (MD 2.13; 95% −8.48, 12.75; p = 0.69) were not significantly different between the two groups. SGLT2Is could therefore be considered neuroprotective in patients with DN, possibly by considerably increasing the sensory and motor nerve conduction velocity, improving the clinical manifestations of DPN, and reducing sympathetic nervous system activity.

Systematic Review Registration:http://www.crd.york.ac.uk/prospero/, identifier CRD42022312828
A Selective Adenylyl Cyclase 1 Inhibitor Relieves Pain Without Causing Tolerance
Authors: Giacoletti Gianna , Price Tatum , Hoelz Lucas V. B. , Shremo Msdi Abdulwhab , Cossin Samantha , Vazquez-Falto Katerina , Amorim Fernandes Tácio V. , Santos de Pontes Vinícius , Wang Hongbing , Boechat Nubia , Nornoo Adwoa , Brust Tarsis F.
Published: July 2022

Among the ten different adenylyl cyclase isoforms, studies with knockout animals indicate that inhibition of AC1 can relieve pain and reduce behaviors linked to opioid dependence. We previously identified ST034307 as a selective inhibitor of AC1. The development of an AC1-selective inhibitor now provides the opportunity to further study the therapeutic potential of inhibiting this protein in pre-clinical animal models of pain and related adverse reactions. In the present study we have shown that ST034307 relives pain in mouse models of formalin-induced inflammatory pain, acid-induced visceral pain, and acid-depressed nesting. In addition, ST034307 did not cause analgesic tolerance after chronic dosing. We were unable to detect ST034307 in mouse brain following subcutaneous injections but showed a significant reduction in cAMP concentration in dorsal root ganglia of the animals. Considering the unprecedented selectivity of ST034307, we also report the predicted molecular interaction between ST034307 and AC1. Our results indicate that AC1 inhibitors represent a promising new class of analgesic agents that treat pain and do not result in tolerance or cause disruption of normal behavior in mice. In addition, we outline a unique binding site for ST034307 at the interface of the enzyme’s catalytic domain.
A Review on the Antidiabetic Properties of Moringa oleifera Extracts: Focusing on Oxidative Stress and Inflammation as Main Therapeutic Targets
Authors: Mthiyane Fikile T. , Dludla Phiwayinkosi V. , Ziqubu Khanyisani , Mthembu Sinenhlanhla X. H. , Muvhulawa Ndivhuwo , Hlengwa Nokulunga , Nkambule Bongani B. , Mazibuko-Mbeje Sithandiwe E.
Published: July 2022

Moringa oleifera is one of the popular plants that have shown significant health benefits. Certainly, preclinical evidence (predominantly from animal models) summarized in the current review supports the beneficial effects of Moringa oleifera leaf extracts in combating the prominent characteristic features of diabetes mellitus. This includes effective control of blood glucose or insulin levels, enhancement of insulin tissue sensitivity, improvement of blood lipid profiles, and protecting against organ damage under sustained conditions of hyperglycemia. Interestingly, as major complications implicated in the progression of diabetes, including organ damage, Moringa oleifera leaf and seed extracts could efficiently block the detrimental effects of oxidative stress and inflammation in these preclinical models. Notably, these extracts (especially leaf extracts) showed enhanced effects in strengthening intracellular antioxidant defences like catalase, superoxide dismutase, and glutathione to lower lipid peroxidation products and reduce prominent pro-inflammatory markers such as tumor necrosis factor-α, interleukin (1L)-β, IL-6, monocyte chemoattractant protein-1 and nitric oxide synthase. From animal models of diabetes, the common and effective dose of leaf extracts of Moringa oleifera was 100–300 mg/kg, within the treatment duration of 2–8 weeks. Whereas supplementation with approximately 20 g leaf powder of Moringa oleifera for at least 2 weeks could improve postprandial blood glucose in subjects with prediabetes or diabetes. Although limited clinical studies have been conducted on the antidiabetic properties of Moringa oleifera, current findings provide an important platform for future research directed at developing this plant as a functional food to manage diabetic complications.
Therapeutic Implications of FABP4 in Cancer: An Emerging Target to Tackle Cancer
Authors: Sun Naihui , Zhao Xing
Published: July 2022

Metabolic reprogramming is an emerging hallmark of tumor cells. In order to survive in nutrient-deprived environment, tumor cells rewire their metabolic phenotype to provide sufficient energy and build biomass to sustain their transformed state and promote malignant behaviors. Fatty acid uptake and trafficking is an essential part of lipid metabolism within tumor cells. Fatty acid-binding proteins (FABPs), which belongs to a family of intracellular lipid-binding protein, can bind hydrophobic ligands to regulate lipid trafficking and metabolism. In particular, adipocyte fatty acid binding protein (FABP4), one of the most abundant members, has been found to be upregulated in many malignant solid tumors, and correlated with poor prognosis. In multiple tumor types, FABP4 is critical for tumor proliferation, metastasis and drug resistance. More importantly, FABP4 is a crucial driver of malignancy not only by activating the oncogenic signaling pathways, but also rewiring the metabolic phenotypes of tumor cells to satisfy their enhanced energy demand for tumor development. Thus, FABP4 serves as a tumor-promoting molecule in most cancer types, and may be a promising therapeutic target for cancer treatment.
Circulatory Metabolomics Reveals the Association of the Metabolites With Clinical Features in the Patients With Intrahepatic Cholestasis of Pregnancy
Authors: Liu Wenhu , Wang Qiang , Chang Jinxia , Bhetuwal Anup , Bhattarai Nisha , Ni Xin
Published: July 2022

Objective: Intrahepatic cholestasis of pregnancy (ICP) is associated with an increased risk of adverse pregnancy to the mother and fetus. As yet, the metabolic profiles and the association of the clinical features remain obscure.

Methods: Fifty-seven healthy pregnant women and 52 patients with ICP were recruited in this study. Plasma samples were collected from pregnancies who received prenatal care between 30 and 36 weeks. Untargeted metabolomics to portray the metabolic profiles were performed by LC/MS. Multivariate combined with the univariate analysis was performed to screen out differential metabolites between the ICP and control groups. A de-biased sparse partial correlation (DSPC) network analysis of differential metabolites was conducted to explore the potential mutual regulation among metabolites on the basis of de-sparsified graphical lasso modeling. The pathway analysis was carried out using MetaboAnalyst. Linear regression and Pearson correlation analysis was applied to analyze correlations of bile acid levels, metabolites, newborn weights, and pregnancy outcomes in ICP patients.

Results: Conspicuous metabolic changes and choreographed metabolic profiles were disclosed: 125 annotated metabolites and 18 metabolic pathways were disturbed in ICP patients. DSPC networks indicated dense interactions among amino acids and their derivatives, bile acids, carbohydrates, and organic acids. The levels of total bile acid (TBA) were increased in ICP patients with meconium-stained amniotic fluid (MSAF) compared with those without MSAF. An abnormal tryptophan metabolism, elevated long chain saturated fatty acids and estrone sulfate levels, and a low-antioxidant capacity were relevant to increased bile acid levels. Newborn weights were significantly associated with the levels of bile acids and some metabolites of amino acids.

Conclusion: Our study revealed the metabolomic profiles in circulation and the correlation of the metabolites with clinical features in ICP patients. Our data suggest that disturbances in metabolic pathways might be associated with adverse pregnancy outcomes.
Early Prediction of Hemodynamic Shock in Pediatric Intensive Care Units With Deep Learning on Thermal Videos
Authors: Vats Vanshika , Nagori Aditya , Singh Pradeep , Dutt Raman , Bandhey Harsh , Wason Mahika , Lodha Rakesh , Sethi Tavpritesh
Published: July 2022

Shock is one of the major killers in intensive care units, and early interventions can potentially reverse it. In this study, we advance a noncontact thermal imaging modality for continuous monitoring of hemodynamic shock working on 1,03,936 frames from 406 videos recorded longitudinally upon 22 pediatric patients. Deep learning was used to preprocess and extract the Center-to-Peripheral Difference (CPD) in temperature values from the videos. This time-series data along with the heart rate was finally analyzed using Long-Short Term Memory models to predict the shock status up to the next 6 h. Our models achieved the best area under the receiver operating characteristic curve of 0.81 ± 0.06 and area under the precision-recall curve of 0.78 ± 0.05 at 5 h, providing sufficient time to stabilize the patient. Our approach, thus, provides a reliable shock prediction using an automated decision pipeline that can provide better care and save lives.
Blood Pressure Response and Pulse Arrival Time During Exercise Testing in Well-Trained Individuals
Authors: Heimark Sondre , Eitzen Ingrid , Vianello Isabella , Bøtker-Rasmussen Kasper G. , Mamen Asgeir , Hoel Rindal Ole Marius , Waldum-Grevbo Bård , Sandbakk Øyvind , Seeberg Trine M.
Published: July 2022

Introduction: There is a lack of data describing the blood pressure response (BPR) in well-trained individuals. In addition, continuous bio-signal measurements are increasingly investigated to overcome the limitations of intermittent cuff-based BP measurements during exercise testing. Thus, the present study aimed to assess the BPR in well-trained individuals during a cycle ergometer test with a particular focus on the systolic BP (SBP) and to investigate pulse arrival time (PAT) as a continuous surrogate for SBP during exercise testing.

Materials and Methods: Eighteen well-trained male cyclists were included (32.4 ± 9.4 years; maximal oxygen uptake 63 ± 10 ml/min/kg) and performed a stepwise lactate threshold test with 5-minute stages, followed by a continuous test to voluntary exhaustion with 1-min increments when cycling on an ergometer. BP was measured with a standard automated exercise BP cuff. PAT was measured continuously with a non-invasive physiological measurements device (IsenseU) and metabolic consumption was measured continuously during both tests.

Results: At lactate threshold (281 ± 56 W) and maximal intensity test (403 ± 61 W), SBP increased from resting values of 136 ± 9 mmHg to maximal values of 219 ± 21 mmHg and 231 ± 18 mmHg, respectively. Linear within-participant regression lines between PAT and SBP showed a mean r2 of 0.81 ± 17.

Conclusion: In the present study focusing on the BPR in well-trained individuals, we observed a more exaggerated systolic BPR than in comparable recent studies. Future research should follow up on these findings to clarify the clinical implications of the high BPR in well-trained individuals. In addition, PAT showed strong intra-individual associations, indicating potential use as a surrogate SBP measurement during exercise testing.
Effects of Different Environment-Friendly Gibberellic Acid Microcapsules on Herbicide Injury of Wheat
Authors: Zhang Guisen , Ma Tao , Cheng Yong , Wang Jianing , Liu Lang , Zhang Baojun
Published: July 2022

Environmentally friendly microcapsules are becoming more and more widely used due to the increasing demand for environmental safety in pesticides. To compare the impact of differences in wheat herbicide phytotoxicity of different gibberellic acid microcapsule suspensions, two microcapsule suspensions were separately formulated using the phase transfer method and the in situ polymerization method, and the key performance indicators are the size of microcapsules and the degree of encapsulation. Meanwhile, through field trials, the pharmacological and detoxification effects of different types of microcapsule suspensions on the herbicide methyldisulfuron in wheat fields were compared. The microcapsule suspension was prepared by the phase transfer method and the particle sizes D10, D50, and D90 are 0.990, 2.136, and 5.201 μm, respectively; the microcapsule suspension was prepared by the in situ polymerization, the particle sizes D10, D50, and D90 are 4.365, 8.547, and 16.782 μm, respectively. The encapsulation rate of the microcapsules prepared by the phase transfer method and the in situ polymerization method was 86.9% and 91.2%, being higher than 80%, the national standard for capsules. Meanwhile, the release rate conforms to first-order release kinetics in 0–4 days and zero-order release kinetics in 5–28 days. The plot trials' result showed that the detoxification effect of the microcapsules prepared by the in situ polymerization method was significantly better than the detoxification effect of the microcapsules prepared by the phase transfer method and the control agent. The growth index of wheat was higher than that of the untreated check after using the agent.
Grain Nutrients Variability in Pigeonpea Genebank Collection and Its Potential for Promoting Nutritional Security in Dryland Ecologies
Authors: Susmitha Dhanapal , Kalaimagal Thiyagarajan , Senthil Ramachandran , Vetriventhan Mani , Manonmani Swaminathan , Jeyakumar Prabhakaran , Anita Bellie , Reddymalla Surender , Choudhari Pushpajeet L. , Nimje Chetna A. , Peerzada Ovais H. , Arveti Venkata Narayana , Azevedo Vania C. R. , Singh Kuldeep
Published: July 2022

Pigeonpea, a climate-resilient legume, is nutritionally rich and of great value in Asia, Africa, and Caribbean regions to alleviate malnutrition. Assessing the grain nutrient variability in genebank collections can identify potential sources for biofortification. This study aimed to assess the genetic variability for grain nutrients in a set of 600 pigeonpea germplasms conserved at the RS Paroda Genebank, ICRISAT, India. The field trials conducted during the 2019 and 2020 rainy seasons in augmented design with four checks revealed significant differences among genotypes for all the agronomic traits and grain nutrients studied. The germplasm had a wider variation for agronomic traits like days to 50% flowering (67–166 days), days to maturity (112–213 days), 100-seed weight (1.69–22.17 g), and grain yield per plant (16.54–57.93 g). A good variability was observed for grain nutrients, namely, protein (23.35–29.50%), P (0.36–0.50%), K (1.43–1.63%), Ca (1,042.36–2,099.76 mg/kg), Mg (1,311.01–1,865.65 mg/kg), Fe (29.23–40.98 mg/kg), Zn (24.14–35.68 mg/kg), Mn (8.56–14.01 mg/kg), and Cu (7.72–14.20 mg/kg). The germplasm from the Asian region varied widely for grain nutrients, and the ones from African region had high nutrient density. The significant genotype × environment interaction for most of the grain nutrients (except for P, K, and Ca) indicated the sensitivity of nutrient accumulation to the environment. Days to 50% flowering and days to maturity had significant negative correlation with most of the grain nutrients, while grain yield per plant had significant positive correlation with protein and magnesium, which can benefit simultaneous improvement of agronomic traits with grain nutrients. Clustering of germplasms based on Ward.D2 clustering algorithm revealed the co-clustering of germplasm from different regions. The identified top 10 nutrient-specific and 15 multi-nutrient dense landraces can serve as promising sources for the development of biofortified lines in a superior agronomic background with a broad genetic base to fit the drylands. Furthermore, the large phenotypic data generated in this study can serve as a raw material for conducting SNP/haplotype-based GWAS to identify genetic variants that can accelerate genetic gains in grain nutrient improvement.
CRISPR/Cas9-Mediated Efficient Targeted Mutagenesis in Sesame (Sesamum indicum L.)
Authors: You Jun , Li Donghua , Yang Li , Dossou Senouwa Segla Koffi , Zhou Rong , Zhang Yanxin , Wang Linhai
Published: July 2022

The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) system has been widely utilized for targeted genome modification in a wide range of species. It is a powerful genome editing technology, providing significant benefits for gene functional research and molecular breeding. However, to date, no study has applied this genome editing tool to sesame (Sesamum indicum L.), one of the most ancient and important oil crops used widely in diverse industries such as food and medicine. Herein, the CRISPR/Cas9 system along with hairy root transformation was used to induce targeted mutagenesis in sesame. Two single guide RNAs (sgRNAs) were designed to target two sesame cytochrome P450 genes (CYP81Q1 and CYP92B14), which are the key biosynthetic gene of sesamin and sesamolin, respectively. Sequencing data illustrated the expected InDel mutations at the target sites, with 90.63 and 93.33% mutation frequency in CYP81Q1 and CYP92B14, respectively. The most common editing event was single nucleotide deletion and insertion. Sequencing of potential off-target sites of CYP92B14-sgRNA showed no off-target events in cases of three mismatches. High-performance liquid chromatography analysis showed that sesamin and sesamolin biosynthesis was effectively disrupted in the mutated hairy roots, confirming the crucial role of CYP81Q1 and CYP92B14 in sesame lignan biosynthesis. These results demonstrated that targeted mutagenesis was efficiently created by the CRISPR/Cas9 system, and CRISPR/Cas9 coupled with hairy root transformation is an effective tool for assessing gene functions in sesame.
Social Cognition in Adult ADHD: A Systematic Review
Authors: Morellini Lucia , Ceroni Martino , Rossi Stefania , Zerboni Giorgia , Rege-Colet Laura , Biglia Elena , Morese Rosalba , Sacco Leonardo
Published: July 2022

The aim of this systematic review was to collect and align the research on social cognition impairments in adults with Attention-deficit/hyperactivity disorder (ADHD). In particular, we selected and analyzed papers on emotion recognition and processing, Theory of Mind (TOM), empathy, and other facets of social cognition as decision making. We identified 16 papers published between 2012 and 2022 which meet inclusion criteria. Papers search, selection, and extraction followed the PRISMA guidelines. In order to summarize data from papers, we used a narrative synthesis approach. Results show different evidence of impairment in social cognition domains in adults with ADHD. Our systematic review suggests the importance of promoting more research on this topic because it is essential to keep in mind that social cognition plays a central role in socialization and social relationships.
Developing Connectedness to Nature in Urban Outdoor Settings: A Potential Pathway Through Awe, Solitude, and Leisure
Authors: Mateer Timothy J.
Published: July 2022

Outdoor leisure experiences may represent an understudied yet effective pathway to promote connectedness to nature for urban park visitors. In contrast to outdoor recreation, this critical essay argues outdoor leisure more heavily emphasizes eudaimonic sentiments and intrinsic motivation in comparison with the goal-oriented and hedonic nature of outdoor recreation. It is further argued that two specific social psychological constructs, awe and solitude, may be especially useful in promoting leisure experiences in urban outdoor spaces. Relevant philosophical and social psychological literature is reviewed and synthesized to outline how land managers and environmental educators may facilitate experiences of awe and solitude to better promote contexts for experiencing outdoor leisure in urban parks. Specifically, reviewed literature suggests that utilizing the recreation opportunity spectrum framework and co-creative processes may be an effective path forward in better supporting urban park environments that are conducive to awe, solitude, and leisure. The review and synthesis of this research may ultimately guide environmental educators, land managers, and researchers in ways to more effectively support connectedness to nature via outdoor leisure experiences as an outcome for visitors to outdoor urban spaces.
The Impact of Partnerships and Information Sharing on Corporate Sustainable Performance: A Mediation Model Moderated by Government Support
Authors: Yue Longfei , Ye Meng , Chen Qing
Published: July 2022

Based on the theory of strategic alliances and social networks, this article empirically studies the relationship between partnership, information sharing, and sustainable performance through a questionnaire survey of Chinese sports equipment manufacturers. The findings show that partnerships have a positive impact on sustainable performance; that information sharing plays a role in mediating the relationships between trust, cooperation, and sustainable performance; and that government support can positively impact the effect of partnerships on sustainable performance. Through empirical research, this article proves the mechanism of the impact of partnership on alliance performance, further expands the theoretical basis for enterprises’ establishment of strategic alliances, and has important enlightening significance for enterprises within alliances aiming to rationally use the networks inside and outside their alliances to obtain knowledge and resources and improve their sustainable performance.
The Evolutionary Game of Post-conflict Management for New Generation of Construction Workers in China: The Mediating Role of Foremen
Authors: Peng Junlong , Zhang Qi
Published: July 2022

The emotional perception of the new generation of Chinese construction workers is becoming stronger, and the traditional punishment-type management model is gradually failing. In order to address the safety hazards caused by the negative emotions generated after workers' conflict events, the motivation of workers to actively participate in the construction of safety climate is increased, and the safety performance of construction projects is enhanced. This paper introduces emotional event theory to assess workers' psychological perceptions and uses foreman as an intermediary for safety management to analyze the decision-making process between managers and work-groups in the safety management process. By establishing a tripartite evolutionary game model of manager, foreman, and worker, the evolutionary differences among the three parties when the manager is strict or appeasing are examined. The results of the study showed that managers who showed appeasement were more effective than those who showed stringency in accomplishing the safety goals of the project. As the workers' psychological perception index increased, workers were more inclined to adopt aggressive strategies, and their behavior was more influenced by their own moral identity as well as the foreman's attitude under the manager's appeasement attitude. This study can provide managers with suggestions on how to handle the situation after a conflict, which can help regulate the behavior of construction teams and eliminate safety risks.
Understanding the Association Between Intolerance of Uncertainty and Problematic Smartphone Use: A Network Analysis
Authors: Liu Chang , Ren Lei , Li Kuiliang , Yang Wei , Li Ye , Rotaru Kristian , Wei Xinyi , Yücel Murat , Albertella Lucy
Published: July 2022

Background

Existing research has demonstrated that intolerance of uncertainty (IU) is associated with problematic smartphone use (PSU). However, little is known about how different IU components such as uncertainty-related beliefs, emotions, and behaviors may impact on different PSU symptoms.

Methods

Extending previous research, the current study examined the specific associations between IU components and PSU symptoms via a symptom-level network approach. A regularized partial correlation network consisting of different IU components and PSU symptoms was estimated among 1,849 Chinese university students. We examined pathways and influential nodes (i.e. central components/symptoms and bridge components/symptoms) within the IU-PSU network.

Results

The strongest pathway linking IU and PSU was between emotional reactions to uncertainty and coping-motivated smartphone use. Importantly, emotional reactions toward not having enough information (a reflection of emotional reactions to uncertainty) may act as both a central and a bridge component in maintaining the whole IU-PSU network.

Conclusions

The results are in line with the I-PACE model and highlight that PSU may be a coping response for negative emotions derived from uncertainty. Finally, the current findings highlight the potential of interventions targeting intolerance of uncertainty for reducing PSU.
EEG-FCV: An EEG-Based Functional Connectivity Visualization Framework for Cognitive State Evaluation
Authors: Zeng Hong , Jin Yanping , Wu Qi , Pan Deng , Xu Feifan , Zhao Yue , Hu Hua , Kong Wanzeng
Published: July 2022

Electroencephalogram (EEG)-based tools for brain functional connectivity (FC) analysis and visualization play an important role in evaluating brain cognitive function. However, existing similar FC analysis tools are not only visualized in 2 dimensions (2D) but also are highly prone to cause visual clutter and unable to dynamically reflect brain connectivity changes over time. Therefore, we design and implement an EEG-based FC visualization framework in this study, named EEG-FCV, for brain cognitive state evaluation. EEG-FCV is composed of three parts: the Data Processing module, Connectivity Analysis module, and Visualization module. Specially, FC is visualized in 3 dimensions (3D) by introducing three existing metrics: Pearson Correlation Coefficient (PCC), Coherence, and PLV. Furthermore, a novel metric named Comprehensive is proposed to solve the problem of visual clutter. EEG-FCV can also visualize dynamically brain FC changes over time. Experimental results on two available datasets show that EEG-FCV has not only results consistent with existing related studies on brain FC but also can reflect dynamically brain FC changes over time. We believe EEG-FCV could prompt further progress in brain cognitive function evaluation.
Life Satisfaction and Suicidal Ideation Among Chinese College Students During the Recurrent Outbreak of COVID-19: A Moderated Mediation Model
Authors: Yu Zhijun , Liu Haidong , Ye Baojuan , Tang Chunyan , Huang Dawu , Liu Lu
Published: July 2022

The present study examined a moderated mediation model between life satisfaction and suicidal ideation among Chinese college students during the recurrent outbreak of COVID-19. Seven hundred and ninety college students participated in the study and completed questionnaires on life satisfaction, suicidal ideation, depression, and gratitude. Findings indicated that (1) Depression played a partial mediating role between life satisfaction and suicidal ideation. Life satisfaction was not only directly affected suicidal ideation, but also indirectly affected suicidal ideation through the mediating effect of college students' depression; (2) Gratitude played a moderating role in the effect of life satisfaction on depression, and the link between life satisfaction and suicide ideation was only significant for those with higher gratitude. This study provides practical implications for the prevention of suicidal behavior among Chinese college students during the recurrent outbreak of COVID-19.
The Complex Interplay Between Physical Activity and Recovery Styles in Patients With Severe Mental Disorders in a Real-World Multicentric Study
Authors: Sampogna Gaia , Luciano Mario , Di Vincenzo Matteo , Andriola Ileana , D'Ambrosio Enrico , Amore Mario , Serafini Gianluca , Rossi Alessandro , Carmassi Claudia , Dell'Osso Liliana , Di Lorenzo Giorgio , Siracusano Alberto , Rossi Rodolfo , Fiorillo Andrea ,   Working Group LIFESTYLE
Published: July 2022

Compared with the general population, people with severe mental disorders have significantly worse physical health and a higher mortality rate, which is partially due to the adoption of unhealthy lifestyle behaviors, such as heavy smoking, use of alcohol or illicit drugs, unbalanced diet, and physical inactivity. These unhealthy behaviors may also play a significant role in the personal and functional recovery of patients with severe mental disorders, although this relationship has been rarely investigated in methodologically robust studies. In this paper, we aim to: a) describe the levels of physical activity and recovery style in a sample of patients with severe mental disorders; b) identify the clinical, social, and illness-related factors that predict the likelihood of patients performing physical activity. The global sample consists of 401 patients, with a main psychiatric diagnosis of bipolar disorder (43.4%, N = 174), psychosis spectrum disorder (29.7%; N = 119), or major depression (26.9%; N = 118). 29.4% (N = 119) of patients reported performing physical activity regularly, most frequently walking (52.1%, N = 62), going to the gym (21.8%, N = 26), and running (10.9%, N = 13). Only 15 patients (3.7%) performed at least 75 min of vigorous physical activity per week. 46.8% of patients adopted sealing over as a recovery style and 37.9% used a mixed style toward integration. Recovery style is influenced by gender (p < 0.05) and age (p < 0.05). The probability to practice regular physical activity is higher in patients with metabolic syndrome (Odds Ratio - OR: 2.1; Confidence Interval - CI 95%: 1.2–3.5; p < 0.050), and significantly lower in those with higher levels of anxiety/depressive symptoms (OR: 0.877; CI 95%: 0.771–0.998; p < 0.01). Globally, patients with severe mental disorders report low levels of physical activities, which are associated with poor recovery styles. Psychoeducational interventions aimed at increasing patients' motivation to adopt healthy lifestyle behaviors and modifying recovery styles may improve the physical health of people with severe mental disorders thus reducing the mortality rates.