bluuebunny/crossref_metadata_2025 huggingface dataset:
MiR-328-3p promotes TGF-β1-induced proliferation, migration, and inflammation of airway smooth muscle cells by regulating the PTEN/Akt pathway
Authors: Gao Jie , Wu Xiao-Lei
Published: March 2023

Background: Recent studies have shown that the up-regulation of microRNA miR-328-3p expression increases seasonal allergy and asthma symptoms in children, but the specific mechanism remains unclear. Therefore, the aim of this study was to explore the role and mechanism of -miR-328-3p in transforming growth factor (TGF)-β1-induced airway smooth muscle cells (ASMCs).
Methods: The effect of TGF-β1 on the expression of miR-328-3p in ASMCs was examined by real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR). Cells proliferation, migration, and inflammatory factors in TGF-β1-induced ASMCs were measured by cell counting kit-8 (CCK-8), transwell, and enzyme-linked immunosorbent assay (ELISA), respectively. Besides, TargetScan was used to predict phosphatase and tensin homolog (PTEN), the downstream target of miR-328-3p; double-luciferase reporter assay, western blot, and qRT-PCR were used to verify the targeting relationship between miR-328-3p and PTEN; western blot was also used to examine the effects of PTEN and miR-328-3p knockdown on the expression levels of PTEN, Akt, and p-Akt proteins.
Results: The expression of miR-328-3p was up-regulated in TGF-β1-induced ASMCs. Knockdown of miR-328-3p significantly inhibited proliferation, migration, and inflammation of ASMCs induced by TGF-β1 and decreased levels of tumor necrosis factor (TNF)-α and interleukin (IL)-1β. The dual--luciferase reporter assay results confirmed that PTEN was a target gene of miR-328-3p. Moreover, inhibition of PTEN expression reversed the inhibitory effect of low miR-328-3p expression on -TGF-β1-induced ASMC’s proliferation, migration, and inflammation. In comparison to the knockdown of miR-328-3p alone, the simultaneous knockdown of miR-328-3p with PTEN decreased PTEN protein expression levels and increased p-Akt/Akt ratio in TGF-β1-induced ASMCs.
Conclusion: Through regulating the expression of PTEN and the activity of Akt signaling pathway, miR-328-3p promotes TGF-β1-induced proliferation, migration, and inflammation of ASMCs.
Investigating antigenic features of the SARS-CoV-2 isolated in Russian Federation in 2021–2022 by hyperimmune mouse serum neutralisation
Authors: Zaykovskaya Anna V. , Evseenko Vasily A. , Olkin Sergey Е. , Pyankov Oleg V.
Published: March 2023

Introduction. The rapid spread of a new coronavirus infection among populations in many countries worldwide has contributed to the genetic evolution of the virus, resulting in the emergence of multiple genetic variants of the SARS-CoV-2 coronavirus. Mutations in the viral genome can affect the ability of the virus to bypass the immune system and complicate development of diagnostic and prophylactic drugs. Data on the neutralizing activity of the sera obtained against previously circulating genetic variants of the virus in relation to current SARS-CoV-2 strains may serve as a scientific basis for the selection of the antigens in vaccine development. The aim of this work was to study cross-reactivity of SARS-CoV-2 coronavirus strains belonging to different genetic variants, which were isolated in the territory of the Russian Federation during 20202022 in the neutralization reaction using mouse hyperimmune sera. Materials and methods. Ten strains of SARS-CoV-2 coronavirus belonging to different genetic variants were used (three non-VOC strains, alpha, beta, gamma, delta, delta+AY, omicron 1 and omicron 2). The hCoV-19/Australia/VIC01/2020 strain (Wuhan) was included in the study as a prototypical variant. BALBc mice were immunized with inactivated concentrated antigen mixed with a 1:1 adjuvant, which was a virus-like immunostimulatory complex based on Quillaja saponaria (Quillaja saponaria). The antibody titer was determined in the neutralization reaction. Results. Essential decrease of neutralizing ability of antibodies specific to non-vOC genetic variants of SARS-CoV-2 coronavirus was revealed against beta VOC and to a lesser degree against alpha and gamma VOC variants. The differences in the neutralizing activity level of antibodies for alpha and beta VOC variants are not significant among themselves, and with gamma VOC variants there are no significant differences. Neutralizing ability of antibodies specific to delta VOC against alpha and beta VOC variants decreased 4-fold. Neutralizing activity of sera obtained to omicron 1 and 2 variants in relation to the prototype coronavirus variant was reduced 18-fold, to the gamma variant 12-fold, to delta variants more than 30-fold; for other variants it was even lower. Conclusions. The results obtained testify to the presence of cross-reactivity between strains of coronavirus belonging to genetic lines Wuhan, alpha, beta, gamma; it is weaker for delta variants. Mutations in the genome of VOC omicron variants led to a significant decrease in antigenic cross-links with earlier genetic variants of the coronavirus. These findings explain the low efficacy of vaccines based on the Wuhan strain, synthetic immunogens, and recombinant proteins based on it against omicron VOC variants, which have caused a rise in morbidity since early 2022, as well as cases of re-infection of humans with new genetic variants of the coronavirus.
Comparative analysis of priority coping strategies of student teachers of various courses
Authors: Volodina Svetlana
Published: March 2023

In the article, the author presents the results of an empirical study conducted as part of a master's thesis. The article is devoted to the study and comparison of the preferred coping strategies of coping behavior of students-teachers of different courses. The study involved students of the second and fourth courses in the amount of 99 people. Analysis of the study conducted by the author using the Mann-Whitney U test showed only one statistically significant difference between groups of students of different courses — in the parameter "Taking responsibility". The level of application of this coping strategy among fourth-year students is significantly higher than among second-year students.
The data obtained in the course of the study allow us to conclude that, in general, the priority coping strategies for the coping behavior of future teachers are constructive strategies that contribute to solving the problem that has arisen, namely: self-control, planning, problem solving and positive reassessment. At the same time, the "flight-avoidance" strategy has a rather high rate in both groups of subjects.
Respondents of both groups least often resort to such destructive coping strategies as confrontational coping and distancing. The results obtained for the parameter "search for social support" have equally high data in both groups of subjects, which allows us to conclude that there is a need for psychological support and support for students.
The conducted comparative static analysis showed that with all the variety of coping strategies of coping behavior periodically used by all participants in the study, fourth-year students slightly, but still more often, use constructive behavior strategies in difficult and conflict situations compared to second-year students.
Nursing protocols to reduce urinary tract infection caused by indwelling catheters: an integrative review
Authors: Miranda Maria Estela de Queiroz , Rosa Marcelo Ricardo , Castro Meire Cristina Novelli e , Fontes Cassiana Mendes Bertoncello , Bocchi Silvia Cristina Mangini
Published: March 2023

ABSTRACT Objectives: to analyze the production of knowledge in research articles about the effectiveness of nursing protocols for reducing indwelling urinary catheter dwell time and catheter-associated urinary tract infection rate in hospitalized adult and older patients. Methods: an integrative review of three full articles, available in the MEDLINE Complete - EBSCO, Scopus and Web of Science databases, from 01/01/2015 to 04/26/2021. Results: the three protocols reduced infection rates, and from the review/synthesis of their knowledge, a level IV body of evidence emerged to compose the nursing care process aimed at reducing indwelling urinary catheter dwell time and catheter-associated urinary tract infection. Final Considerations: this process gathers scientific evidence to support the elaboration of nursing protocols and, consequently, the conduction of clinical trials on its effectiveness in reducing urinary tract infection by indwelling urinary catheter.
Palliative care strategies in the management of people with serious cases of COVID-19
Authors: Prado Eleandro do , Camparoto Camila Wohlenberg , Takemoto Angelica Yukari , Ichisato Sueli Mutsumi Tsukuda , Miguel Maria Emília Grasso Busto , Marcon Sonia Silva
Published: March 2023

ABSTRACT Objectives: to analyze the assistance strategies in palliative care developed in the COVID-19 pandemic for critically ill patients and their families. Methods: an integrative review carried out in August 2021 and updated in April 2022 in the Base de Dados de Enfermagem (BDENF), Cumulative Index to Nursing and Allied Health Literature (CINAHL), Medical Literature Analysis and Retrieval System Online (MEDLINE), US National Library of Medicine (PubMed), Web of Science databases, and presented in the PRISMA flowchart. Results: thirteen works were selected for reading and content analysis, from which emerged the two main themes that reflect the reality evidenced in this context: The sudden advent of COVID-19 with impacts on palliative care; and The strategies used in palliative care to mitigate these impacts. Final Considerations: palliative care is the best strategy for providing health care, used as a comfort to bring relief and comfort to patients and families.
O processo de autocuidado de idosos comunitários no contexto da pandemia de COVID-19
Authors: Lopes Larissa Padoin , Santi Daniela Bulcão , Marques Francielle Renata Danielli Martins , Salci Maria Aparecida , Carreira Lígia , Baldissera Vanessa Denardi Antoniassi
Published: March 2023

RESUMO Objetivos: compreender o processo de autocuidado dos idosos da comunidade no contexto da pandemia de COVID-19. Métodos: trata-se de um estudo explicativo de abordagem qualitativa, com referencial da Teoria Fundamentada nos Dados construtivista, realizada com 18 idosos comunitários. A coleta de dados ocorreu através de entrevistas, e o conteúdo foi analisado por meio da codificação inicial e focalizada. Resultados: foram obtidas duas categorias: “Estabelecendo conexões para apoiar práticas de autocuidado” e “Convivendo com o estigma de grupo de risco”. A partir da interação dessas, emergiu o fenômeno “Exercendo o autocuidado na terceira idade no contexto da pandemia de COVID-19”. Considerações Finais: foi possível identificar como as vivências dos idosos no período de pandemia de COVID-19 repercutiram no seu processo de autocuidado, sendo influenciados por fatores, como informações sobre a doença e os impactos dos estigmas do grupo risco.
Tomographic, microbiological and histological characterization of secondary apical periodontitis: case series
Authors: Mora-Carabalí Marla , Contreras Adolfo , Rodríguez Patricia , Zamora Ingrid , Rodríguez Martha
Published: March 2023

Abstract This case series included a tomographic, microbiological, and histopathological description of 15 secondary apical periodontitis (SAP) lesions obtained by apical microsurgery performed in 10 patients to better understand the etiology and pathogenesis of SAP. Preoperative tomographic analyses were performed through Cone beam computerized tomography - Periapical index (CBCT-PAI), and apical microsurgeries were then carried out. The removed apices were used for microbial culturing and for molecular identification using PCR for the detection of 5 strict anaerobic bacteria (P. gingivalis, P. intermedia, P. nigrescens, T. forsythia, and T.denticola) and 3 viruses Herpes simplex viruses (HSV), Cytomegalovirus (CMG) and Epstein-Barr Virus (EBV) by nested PCR. The removed apical lesions were histologically described. Univariate statistical analyses were performed by using STATA MP/16 (StataCorp LLC, College Station, TX, United States). CBCT-PAI analyses revealed PAI 4 and PAI 5 score lesions that involved cortical plate destruction. Eight SAPs were positive by culture, while nine SAP lesions were positive by PCR. Fusobacterium species were the most frequently cultured organisms in 7 SAP lesions, followed by D. pneumosintes in 3. In contrast, by single PCR, T. forsythia and P. nigrescens were detected in 5 lesions, T. denticola in 4 lesions, and P. gingivalis in 2 lesions. Twelve periapical lesions were granulomas, and the remaining three SAP lesions were radicular cysts. In conclusion, this case series study revealed that secondary apical lesions presented tomographic involvement of PAI 3 to 5, and that most SAP lesions were apical granulomas containing anaerobic and facultative microorganisms.
Effect of aging and cementation systems on the bond strength to root dentin after fiber post cementation
Authors: Nogueira Caio Henrique de Paula , Gelio Mariana Bena , Besegato João Felipe , Ramos Anna Thereza Peroba Rezende , Fernández Eduardo , Kuga Milton Carlos , Saad José Roberto Cury
Published: March 2023

Abstract This study evaluated the effect of aging and cementation of fiber posts using glass ionomer and resin cements on push-out bond strength, failure mode, and resin tag formation. One hundred and twenty bovine incisors were used. After post-space preparation, the specimens were randomly allocated into 12 groups (n = 10) according to the cementation system used: GC - GC Gold Label Luting & Lining); RL - RelyX Luting 2; MC - MaxCem Elite; RU - RelyX U200 and the aging periods (24 hours, 6 months, and 12 months). Slices from the cervical, middle, and apical thirds were obtained and analyzed by push-out bond strength test and confocal laser scanning microscopy. One-way ANOVA and Tukey’s post-hoc test was used at a significance level of 5%. For the push-out bond strength test, no differences among GC, RU, and MC in the cervical and middle thirds were observed, regardless of the period of storage (P > 0.05). In the apical third, GC and RU showed similar bond strength but higher than other groups (P > 0.05). After 12 months, GC showed the highest bond strength (P < 0.05). Bond strength to post-space dentin decreased over time, regardless of the cementation system used. Cohesive failure was the most frequent, regardless of the period of storage, cementation system, and post-space third. Tag formation was similar among all groups. After 12 months, GC showed the highest bond strength values.
Effects of BlueM® against Streptococcus mutans biofilm and its virulence gene expression
Authors: Santos Veronica Canela Estevam dos , Maquera-Huacho Patricia Milagros , Imbriani Maria Júlia Mancim , Minhaco Vivian M. Tellaroli Rodrigues , Spolidorio Denise M. Palomari
Published: March 2023

Abstract This study evaluated the antimicrobial capacity of BlueM® mouthwash against the bacterium Streptococcus mutans and its influence on gbpA gene expression as well as its cytotoxic effect on fibroblast cells. BlueM® showed antimicrobial activity, with MIC and MBC values of 0.005% and 0.01%, respectively. The MBIC was 6.25% for S. mutans. CFU count and confocal microscopy revealed significant effect of BlueM® on S. mutans biofilm pre-formed on dentin surfaces. Interestingly, the analysis of gbpA gene expression indicated a decrease in gene expression after 15 min of treatment with BlueM® at a concentration of 25%. Moreover, BlueM® exhibited low levels of cytotoxicity. In conclusion, our results showed the antimicrobial effectiveness of BlueM® against S. mutans, its ability to modulate the expression of the gbpA gene and its low cytotoxicity. This study supports the therapeutic potential of BlueM® as an alternative agent for the control of oral biofilm.
Mechanism of action of Bioactive Endodontic Materials
Authors: Estrela Carlos , Cintra Luciano Tavares Angelo , Duarte Marco Antônio Hungaro , Rossi-Fedele Giampiero , Gavini Giulio , Damião Sousa-Neto Manoel
Published: March 2023

Abstract A continuous search for bioactive materials capable of supporting the replacement of damaged pulp tissue, with effective sealing potential and biocompatibility, has represented the attention of studies over the last decades. This study involves a narrative review of the literature developed by searching representative research in PUBMED/MEDLINE and searches in textbooks associated with the mechanism of action of bioactive materials (calcium hydroxide, mineral trioxide aggregate (MTA), and calcium silicate cements). The reflective analysis of the particularities of the chemical elements of these materials, considering the tissue and antibacterial mechanism of action, allows a better understanding of the characteristics and similarities in their tissue responses. Calcium hydroxide paste remains the antibacterial substance of choice as intracanal dressing for the treatment of root canal system infections. Calcium silicate cements, including MTA, show a favorable biological response with the stimulation of mineralized tissue deposition in sealed areas when in contact with connective tissue. This is due to the similarity between the chemical elements, especially ionic dissociation, the potential stimulation of enzymes in tissues, and the contribution towards an alkaline environment due to the pH of these materials. The behavior of bioactive materials, especially MTA and the new calcium silicate cements in the biological sealing activity, has been shown to be effective. Contemporary endodontics has access to bioactive materials with similar properties, which can stimulate a biological seal in lateral and furcation root perforations, root-end fillings and root fillings, pulp capping, pulpotomy, apexification, and regenerative endodontic procedures, in addition to other clinical conditions.
Development of candy “paçoca” from pequi almond
Authors: Prado Núbia Francisca de Oliveira , Siqueira Rafaela Anunciação , Silva Rosana Maria Pereira , Costa Danusa Silva da , Cagnin Caroline , Plácido Geovana Rocha
Published: March 2023

ABSTRACT: The pequi has an almond with interesting chemical characteristics, such as considerable proportions of proteins and lipids that can be applied in several products aiming to use this agro-industrial residue. This research tookadvantage of the almond for the development of sweet paçoca with different concentrations of pequi almond, containing 0% (PA), 25% (P1), 50% (P2), 75% (P3), and 100% (P4) in place of peanuts. The paçocas were evaluated for proximate parameters and water activity, mineral content, color parameters, microbiological characteristics, and texture profile. The results for aw ranged from 0.48 to 0.52. In the proximate composition, it was observed that with the increase of the almond flour there was a difference in the ash, moisture, and protein contents. Due to the almond roasting process, the paçocas of pequi presented the darkest color. Pequi almond paçocas showed better protein value than peanut paçoca and higher levels of P and Mg. In addition, they present better elasticity, chewability, and cohesiveness.
Risco ambiental provocado por resíduos de medicamentos na cidade do Rio de Janeiro, Brasil, durante a pandemia por SARS-Cov19
Authors: Souza Carla Patricia Figueiredo Antunes de , Kligerman Débora Cynamon , Bezerra Giselle Mendes , Oliveira Jaime Lopes da Mota
Published: March 2023

Resumo Foi avaliada a relação entre a distribuição de medicamentos usados na pandemia por SARS-COV-19 no município do Rio de Janeiro e o nível de risco ambiental estimado provocado por seus resíduos. Foi coletada a quantidade de medicamentos distribuídos pelas unidades de atenção primária à saúde (APS) entre 2019 e 2021. O quociente de risco (QR) correspondeu à razão entre a concentração ambiental preditiva estimada (PECest), obtida pelo consumo e excreção de cada fármaco, e a sua concentração preditiva não efetiva (PNEC). Os PECest da azitromicina e da ivermectina aumentaram entre 2019 e 2020, tendo uma queda em 2021 provavelmente devido ao desabastecimento. Já o da dexclorfeniramina (DEX) e da fluoxetina (FLU) tiveram uma queda, retornando o crescimento em 2021. Enquanto o PECest do diazepam (DIA) aumentou ao longo desses três anos, o etinilestradiol (EE2) diminuiu, possivelmente pela priorização da APS no tratamento da COVID-19. Os maiores QR foram de FLU, EE2 e AZI. O padrão de consumo desses medicamentos não refletiu seu risco ambiental, pois os mais consumidos possuem baixa toxicidade. Vale destacar que alguns dados podem estar subestimados devido ao incentivo que foi dado durante a pandemia para o consumo de determinados grupos de fármacos.
Classificação de risco para transmissão de doenças imunopreveníveis em Minas Gerais, Brasil: dois anos desde o início da pandemia de COVID-19
Authors: Silva Thales Philipe Rodrigues da , Vimieiro Aline Mendes , Gusmão Josianne Dias , Souza Janaina Fonseca Almeida , Lachtim Sheila Aparecida Ferreira , Vieira Ed Wilson Rodrigues , Silva Tércia Moreira Ribeiro da , Matozinhos Fernanda Penido
Published: March 2023

Resumo O objetivo é analisar a classificação de risco de transmissão de doenças imunopreveníveis nos 853 municípios de Minas Gerais (MG) após dois anos de início da pandemia de COVID-19. Estudo epidemiológico com dados secundários da cobertura vacinal e taxa de abandono de dez imunobiológicos recomendados para crianças menores de 2 anos, no ano de 2021, em MG. Em relação à taxa de abandono, este indicador foi avaliado somente para as vacinas multidoses. Após o cálculo de todos os indicadores, os municípios do estado foram classificados de acordo com o risco de transmissão de doenças imunopreveníveis em cinco estratos. Minas Gerais apresentou 80,9% dos municípios classificados como alto risco para transmissão de doenças imunopreveníveis. Em relação à homogeneidade das coberturas vacinais (HCV), os municípios de grande porte apresentaram a maior porcentagem de HCV classificada como muito baixa e 100% desses municípios foram classificados como de alto ou muito alto risco para transmissão de doenças imunopreveníveis, com significância estatística. A utilização de indicadores de imunização por município é efetiva para o delineamento do cenário de cada território e a proposição de políticas públicas em saúde visando o aumento das coberturas vacinais.
The COVID-19 pandemic through the lens of humanitarian logistics
Authors: Silveira Stelacelly Coelho Toscano , Ferreira Filho Hélio Raymundo , Pontes Altem Nascimento , Lopes Harlenn dos Santos , Manfrini Gisele Cristina
Published: March 2023

Abstract An integrative literature review (ILR) was conducted to identify the relationship between humanitarian logistics and the development of the COVID-19 pandemic based on research in the SCOPUS, MEDLINE and ENEGEP databases in April and May 2022. In all, 61 articles were evaluated according to the following criteria: original article or review of literature published in a scientific journal; abstract and full text available; article on humanitarian logistics in relation to the COVID-19 pandemic. The resulting sample comprised eleven publications organized and analyzed through a synthesis matrix, where 72% were published in international journals and mostly in 2021 (56%). The presence of the supply chain defines the course of action of economic and social sectors, which in turn determine, by means of an interdisciplinary approach, humanitarian operations in the face of the COVID-19 pandemic. The lack of studies narrows down humanitarian logistics to mitigate the impacts caused by these disasters, both in the context of the current pandemic and in future events of the same nature. However, as a global emergency, it suggests the need to increase scientific knowledge on the subject of humanitarian logistics related to disaster events.
Factors associated with the incidence and worsening of back pain during the first wave of COVID-19 in Brazil
Authors: Romero Dalia Elena , Muzy Jessica , Maia Leo Ramos , Almeida Wanessa da Silva de , Silva Danilo Rodrigues Pereira da , Malta Deborah Carvalho , Souza Junior Paulo Roberto Borges de
Published: March 2023

Abstract The article aims to estimate the incidence and worsening of back pain (BP) during the first wave of COVID-19 in Brazil, as well as to investigate demographic, socioeconomic factors and associated changes in living conditions. ConVid - Behavior Research, applied between April and May 2020, was used as data source. The number and distribution of respondents who developed BP and those who had a worsening of the preexisting problem, their 95% confidence intervals and Pearson’s Chi-square test were estimated. The odds ratio of developing BP or worsening a preexisting problem was also estimated using multiple logistic regression models. Pre-existing BP was reported by 33.9% (95%CI 32.5-35.3) of respondents and more than half (54.4%; 95%CI 51.9-56.9) had worsened. The cumulative incidence of BP in the first wave of the pandemic was 40.9% (95%CI 39.2-42.7). Being a woman, the perceived increase in housework and the frequent feeling of sadness or depression were associated with both outcomes. Socioeconomic factors were not associated with any of outcome. The high incidence and worsening of BP during the first wave reveal the need for studies in more recent periods, given the long duration of the pandemic.
Family instability in childhood affects language and memory in adulthood: results from the Pró-Saúde Study, Brazil
Authors: Araújo Larissa Fortunato , Faerstein Eduardo
Published: March 2023

Abstract The study aims to investigate associations between adverse childhood psychosocial exposures and declarative memory, language, and executive function in adults with secondary schooling or more and without dementia. In 361 participants from the Pró-Saúde Study, we estimated associations between maternal educational attainment, principal source of the family´s income, food insecurity, and childhood family structure and performance in learning, word recall, and semantic and phonemic verbal fluency tests using multiple linear regression models. Individuals whose mother was the family breadwinner (mean difference: -1.97, 95%CI: -3.27; -0.72) and head-of-household (mean difference: -1.62, 95%CI: -2.89; -0.35) or who lived with a non-parental caregiver or in institutions in childhood (mean difference: -2.19, 95%CI: -4.29; -0.09) showed a reduction in the mean number of words in language and memory in adulthood. The results provide further evidence of the effect of adverse exposures in childhood. Without effective interventions, such exposures are likely to have far-reaching impacts on cognition.
Fila única de leitos e a pandemia de COVID-19: atuação do Poder Legislativo Federal no ano de 2020
Authors: Rodrigues Ana Paula Nogueira , Alves Sandra Mara Campos , Delduque Maria Célia
Published: March 2023

Resumo A pesquisa investigou proposições legislativas sobre fila única de internações e leitos de UTI no âmbito do Poder Legislativo Federal no primeiro ano da pandemia. Tratou-se de estudo exploratório, qualitativo e de base documental que analisou projetos de leis sobre o tema. Os resultados foram organizados conforme o perfil dos autores e o conteúdo qualitativo dos projetos. Preponderaram parlamentares do sexo masculino, filiados a partidos de esquerda e com formação profissional em áreas que não a da saúde. A maioria das proposições tratou de fila única geral, especificando leitos hospitalares, gestão mista e indenização mediante tabela SUS. A Câmara dos Deputados apresentou mais projetos, porém pouco tem avançado em suas tramitações. Entre os projetos analisados, apenas um foi priorizado na Comissão Externa de Enfrentamento à COVID-19. Concluiu-se que o Poder Legislativo Federal perdeu, uma vez mais, oportunidade valiosa de legislar para o futuro e preparar o país com um arcabouço normativo capaz de enfrentar emergências sanitárias ainda desconhecidas, mas que exigirão muito dos gestores e do SUS.
Asistencia a familias de niños con Trastornos del Espectro Autista: percepciones del equipo multidisciplinario
Authors: Bonfim Tassia de Arruda , Giacon-Arruda Bianca Cristina Ciccone , Galera Sueli Aparecida Frari , Teston Elen Ferrraz , Nascimento Francisneide Gomes Pego Do , Marcheti Maria Angélica
Published: March 2023

Resumen Objetivo: resumir la atención que brindan los profesionales de la salud, en los diferentes niveles de atención, a las familias de niños con Trastornos del Espectro Autista. Método: estudio cualitativo, basado en el marco teórico filosófico de la Atención Centrada en la Familia, desarrollado con 22 profesionales de tres equipos multidisciplinarios de servicios de la Red de Atención de la Salud en un municipio del estado de Mato Grosso do Sul, Brasil. Los datos fueron recolectados a través de dos grupos focales con cada equipo y organizados con la ayuda del software Atlas.ti 8 Qualitative Data Analysis ® y sometidos al Análisis de Contenido Temático. Resultados: los hallazgos muestran acciones enfocadas en situaciones específicas, principalmente en las demandas y necesidades que genera el cuidado del niño y su comportamiento atípico. Factores que influyen en la atención a la familia, como la sobrecarga de trabajo y la poca experiencia profesional, revelan las falencias que tiene la asistencia multidisciplinaria y la invisibilidad de la familia como unidad de atención. Conclusión: es necesario examinar el funcionamiento y la forma en que está organizada la red para la atención multidisciplinaria de los niños y sus familias. Se recomienda implementar acciones de educación continua que contribuyan a la capacitación de los equipos multidisciplinarios para la atención a las familias de niños con espectro autista.
Assistência às famílias de crianças com Transtornos do Espectro Autista: percepções da equipe multiprofissional
Authors: Bonfim Tassia de Arruda , Giacon-Arruda Bianca Cristina Ciccone , Galera Sueli Aparecida Frari , Teston Elen Ferrraz , Nascimento Francisneide Gomes Pego Do , Marcheti Maria Angélica
Published: March 2023

Resumo Objetivo: sintetizar o cuidado prestado por profissionais de saúde, nos diferentes níveis de atenção, às famílias de crianças com Transtornos do Espectro Autista. Método: estudo qualitativo, baseado no referencial teórico filosófico do Cuidado Centrado na Família, desenvolvido com 22 profissionais de três equipes multidisciplinares de serviços da Rede de Atenção à Saúde de um município do estado de Mato Grosso do Sul, Brasil. Os dados foram coletados por meio de dois grupos focais com cada equipe, organizados com apoio do software Atlas.ti 8 Qualitative Data Analysis e submetidos à Análise Temática de Conteúdo. Resultados: as descobertas mostram ações centradas em situações pontuais, principalmente nas demandas e necessidades advindas do cuidado da criança e de seu comportamento atípico. Fatores influenciadores para o cuidado à família, como a sobrecarga de trabalho e a pouca experiência profissional, evidenciam a fragilidade da assistência multiprofissional e a invisibilidade da família enquanto unidade de cuidado. Conclusão: destaca-se a necessidade de rever o funcionamento e modo como a rede para o cuidado multiprofissional da criança e sua família está organizada. Recomenda-se a oferta de ações de educação permanente que contribuam com a qualificação das equipes multiprofissionais no cuidado às famílias de crianças no espectro do autismo.
Impacto de la pandemia de COVID-19 en los trastornos del sueño de profesionales de enfermería
Authors: Andrechuk Carla Renata Silva , Caliari Juliano de Souza , Santos Mariana Alvina dos , Pereira Flávia Helena , Oliveira Henrique Ceretta , Ceolim Maria Filomena
Published: March 2023

Resumen Objetivo: analizar los factores relacionados con los trastornos del sueño que informaron los profesionales de enfermería durante la pandemia de COVID-19. Método: se trata de un estudio transversal y analítico realizado con profesionales de enfermería de todas las regiones de Brasil. Se recolectaron datos sobre caracterización sociodemográfica, condiciones de trabajo y preguntas sobre trastornos del sueño. Para estimar el Riesgo Relativo se utilizó el modelo de regresión de Poisson con medidas repetidas. Resultados: se analizaron 572 respuestas, que revelaron que durante la pandemia predominaron la duración del sueño no ideal, la mala calidad del sueño y los sueños sobre el ambiente laboral, con 75,2%, 67,1% y 66,8% respectivamente, además 523 (91,4%), 440 (76,9%) y 419 (73,2%) profesionales de enfermería manifestaron quejas de dificultad para conciliar el sueño, somnolencia diurna y sueño no reparador durante la pandemia, respectivamente. El riesgo relativo de padecer trastornos del sueño durante la pandemia fue significativo para todas las variables y categorías estudiadas. Conclusión: la duración del sueño no ideal, la mala calidad del sueño, los sueños sobre el ambiente laboral, las quejas de dificultad para conciliar el sueño, la somnolencia diurna y el sueño no reparador fueron los trastornos del sueño predominantes en los profesionales de enfermería durante la pandemia. Estos hallazgos indican posibles consecuencias para la salud, así como para la calidad del trabajo realizado.
Parental presence during invasive pediatric procedures: what does it depend on?
Authors: Palomares González Laura , Hernández Caravaca Iván , Gómez García Carmen Isabel , Sánchez-Solís de Querol Manuel
Published: March 2023

Abstract Objective: family-centered care during invasive procedures has been endorsed by many professional health care organizations. The aim of this study was to evaluate the health professionals’ attitudes towards parental presence during their child’s invasive procedure. Method: pediatric healthcare providers (divided in professional categories and range of ages) from one of the Spain’s largest hospitals were asked to complete a questionnaire and write free-text comments. Results: 227 responded the survey. Most (72%) participants, in their answers, reported that parents are sometimes present during interventions, although there were differences between professional categories in this respect. The procedures in which the parents were present were those considered “less invasive” (96% of cases), while only 4% were present in those considered “more invasive”. The older the professional, the less necessary parental presence was considered. Conclusion: the attitudes towards parental presence during pediatric invasive procedure are influenced by the professional category, the age of the healthcare provider and the invasiveness of the procedure.
Os impactos psicossociais da pandemia de COVID-19 entre mulheres assentadas: estudo longitudinal
Authors: Oliveira Jaqueline Lemos de , Almeida Janaina Cristina Pasquini de , Pauli Antonio Jose Correa de , Moitinho Mara Regina , Fiorati Regina Célia , Souza Jacqueline de
Published: March 2023

Resumo Objetivo: analisar os impactos psicossociais da pandemia de COVID-19 entre mulheres brasileiras de assentamentos rurais. Método: trata-se de um estudo quantitativo longitudinal com 13 mulheres assentadas. Os dados foram coletados entre janeiro de 2020 e setembro de 2021 utilizando questionários sobre a percepção do ambiente social (qualidade de vida, apoio social, autoeficácia), sintomas de transtorno mental comum e aspectos sociodemográficos. Os dados foram analisados por meio de estatística descritiva, análise de agrupamento e de variância. Resultados: foram identificadas condições de vulnerabilidade interseccionadas que, possivelmente, exacerbaram os desafios decorrentes da pandemia. O domínio físico da qualidade de vida oscilou diferentemente e inversamente de acordo com os sintomas de transtorno mental. Quanto ao domínio psicológico, no final do segmento, identificou-se em toda a amostra um incremento ao longo do tempo, pois a percepção das mulheres estava melhor do que antes da pandemia. Conclusão: a piora na saúde física das participantes merece destaque e, provavelmente, pode estar relacionada à dificuldade de acesso aos serviços de saúde neste período bem como ao medo da contaminação. Apesar disso, as participantes apresentaram-se emocionalmente resilientes em todo o período, inclusive, com sinais de melhora em relação aos aspectos psicológicos, sugerindo um possível efeito da organização comunitária do assentamento.
Evaluación enfermera de la ansiedad prequirúrgica pediátrica: estudio cualitativo
Authors: Jerez Molina Carmen , Lahuerta Valls Laura , Fernandez Villegas Victoria , Santos Ruiz Susana
Published: March 2023

Resumen Objetivo: explorar y describir cómo las enfermeras perioperatorias evalúan e interpretan el comportamiento del niño antes de entrar a quirófano, identificando las estrategias que utilizan para minimizar la ansiedad y las propuestas de mejora. Método: estudio cualitativo descriptivo mediante entrevistas semiestructuradas y observación participante de las rutinas diarias. Análisis temático de los datos. El estudio sigue las recomendaciones de criterios para la publicación de artículos de metodología cualitativa Consolidated Criteria for Reporting Qualitative Research. Resultados: cuatro temas surgieron de los datos: a) evaluación de la ansiedad o comunicación estrecha con el niño y su familia; b) valorando lo observado; c) manejando la ansiedad y d) mejorando la evaluación o propuestas de mejora para la práctica diaria. Conclusión: enfermeras evalúan la ansiedad en su práctica diaria de forma observacional utilizando el juicio clínico. La experiencia de la enfermera es determinante en la adecuada evaluación de la ansiedad prequirúrgica del niño. La falta de tiempo entre la espera y el momento de entrar a quirófano, la mala información que tiene el niño y los padres sobre el proceso quirúrgico y la ansiedad de los padres dificultan la evaluación y el manejo correcto de la ansiedad.
Avaliação de enfermagem da ansiedade pré-operatória pediátrica: um estudo qualitativo?
Authors: Jerez Molina Carmen , Lahuerta Valls Laura , Fernandez Villegas Victoria , Santos Ruiz Susana
Published: March 2023

Resumo Objetivo: explorar e descrever como as enfermeiras perioperatórias avaliam e interpretam o comportamento da criança antes de entrar na sala de cirurgia, identificando as estratégias que utilizam para minimizar a ansiedade e as propostas de melhoria. Método: estudo qualitativo descritivo utilizando entrevistas semiestruturadas e observação participante das rotinas diárias. Análise temática dos dados. O estudo segue os critérios recomendados para publicação de artigos da metodologia qualitativa Consolidated Criteria for Reporting Qualitative Research. Resultados: quatro temas emergiram dos dados: a) avaliação da ansiedade ou comunicação próxima com a criança e sua família; b) analisando o que foi observado; c) controlando a ansiedade e d) melhorando a avaliação ou propostas de melhoria na prática diária. Conclusão: as enfermeiras avaliam a ansiedade em sua prática diária por meio da observação e usando julgamento clínico. A experiência da enfermeira é decisiva na avaliação adequada da ansiedade pré-operatória da criança. A falta de tempo entre a espera e o momento de entrar na sala de cirurgia, a escassez de informação que a criança e os pais têm sobre o processo cirúrgico e a ansiedade dos pais, dificultam a avaliação e o controle adequado da ansiedade.
Factores de riesgo para el desarrollo de infección del sitio quirúrgico en cirugía bariátrica: una revisión integradora
Authors: Silva Ana Flávia da , Mendes Karina Dal Sasso , Ribeiro Vanessa dos Santos , Galvão Cristina Maria
Published: March 2023

Resumen Objetivo: evaluar las evidencias sobre los factores de riesgo para el desarrollo de infección de sitio quirúrgico en cirugía bariátrica. Método: revisión integradora. La búsqueda de estudios primarios se realizó en cuatro bases de datos. La muestra estuvo compuesta por 11 investigaciones. La calidad metodológica de los estudios incluidos se evaluó mediante herramientas propuestas por el Joanna Briggs Institute. El análisis y la síntesis de los datos se realizaron de manera descriptiva. Resultados: las tasas de infección del sitio quirúrgico oscilaron entre 0,4% y 7,6%, considerando los resultados de los estudios primarios, en los que los pacientes fueron sometidos a cirugía mediante laparoscopia. En investigaciones con participantes que se sometieron a procedimientos quirúrgicos con diferentes enfoques (abierto, laparoscópico o robótico), las tasas de infección oscilaron entre el 0,9 % y el 12 %. En cuanto a los factores de riesgo para el desarrollo de este tipo de infección, se destacan la profilaxis antibiótica, el sexo femenino, el Índice de Masa Corporal elevado y la hiperglucemia perioperatoria. Conclusión: la realización de la revisión generó un cuerpo de evidencia que refuerza la importancia de implementar medidas efectivas para la prevención y el control de la infección de sitio quirúrgico, por parte de los profesionales de la salud, después de la cirugía bariátrica, promoviendo la mejora de la atención y la seguridad del paciente en el período perioperatorio.
Duração da amamentação e comportamentos alimentares na primeira infância: uma revisão sistemática
Authors: Ergang Barbara Cristina , Caprara Gabriele Luiza , Machado Mirian Benites , Moreira Paula Ruffoni , Hagen Martine Elizabeth Kienzle , Bernardi Juliana Rombaldi
Published: March 2023

Resumo Objetivos: analisar a influência da duração do aleitamento materno no comportamento alimentar em crianças de dois a seis anos. Métodos: esta revisão foi conduzida de acordo com as diretrizes PRISMA. As bases de dados SciELO, Lilacs, Embase e PubMed foram pesquisadas usando uma sintaxe específica, para estudos publicados de 2000 a 2020. O Joanna Briggs Institute Critical Appraisal checklist foi utilizado para avaliar o risco de viés do estudo. Resultados: foram identificados 26.211 artigos, dos quais sete foram incluídos no estudo. Os resultados mostraram associação significativa em quatro estudos. Todos os autores usaram seus próprios questionários para avaliar a exposição à amamentação; não havia uma classificação padrão de duração do aleitamento materno exclusivo e total. A duração do aleitamento materno foi associada à redução da neofobia alimentar, menores escores na subescala de responsividade alimentar e menor comportamento alimentar exigente. Instrumentos validados foram usados predominantemente para avaliar o resultado do comportamento alimentar, no entanto, essa avaliação não foi semelhante entre os estudos. Conclusão: observou-se associação significativa entre a duração da amamentação e o comportamento alimentar em crianças de dois a seis anos. Mais pesquisas devem ser realizadas para descrever os mecanismos envolvidos nesta associação.
Abiotic factors and photosynthetically active photon density affect the physiological mechanisms of jaboticaba
Authors: Coêlho Ester dos S. , Ribeiro João E. da S. , Silva Elania F. da , Silva Toshik I. da , Oliveira Pablo H. de A. , Dias Thiago J. , Barros Júnior Aurélio P. , Silva Daniel V. , Rodriguez Ronald M.
Published: March 2023

ABSTRACT In fruit species, the amount of solar energy absorbed can influence fruit quality; hence, ensuring optimal light distribution management in the canopy of plants is essential. Therefore, the objectives of this study were (i) to analyze the variations in gas exchange through the day and (ii) identify the photosynthetically active photon flux density (PPFD) that promotes higher chlorophyll fluorescence and electron transport rate in jaboticaba seedlings. The experimental design was completely randomized, with treatments consisting of 18 photosynthetic photon flux densities and three evaluations throughout the day. Six replicates were used, with two plants per plot. Gas exchange and chlorophyll a fluorescence in P. peruviana were altered due to fluctuating photosynthetic photon flux density (0; 25; 50; 75; 100; 125; 150; 175; 200; 400; 600; 800; 1,000; 1,200; 1,400; 1,600; 1,800; and 2,000 μmol m-2 s-1) and environmental conditions throughout the day (8:00 a.m., 12:00 and 4:00 p.m.). The higher PPFD (1,384.6 μmol m-2 s-1) and air temperature (39.74 °C) at noon (12:00 p.m.) favored gas exchange in this species. An increase in PPFD of up to 1,000 μmol m-2 s-1 positively influenced the gas exchange and chlorophyll a fluorescence of P. peruviana.
Production of watermelon seedlings in different substrates under salt stress
Authors: Sousa Geocleber G. de , Sousa Henderson C. , Lessa Carla I. N. , Goes Geovana F. , Freire Márcio H. da C. , Souza Maria V. P. de , Gomes Silas P. , Schneider Fernanda
Published: March 2023

ABSTRACT Salt stress affects the production of watermelon (Citrullus lanatus) seedlings. However, substrates with alternative materials in their composition can mitigate the harmful effects on the formation of watermelon seedlings. Thus, the objective of the present study was to evaluate the emergence and production of watermelon seedlings grown in different substrates under salt stress. The research was conducted in an agricultural greenhouse belonging to the Universidade da Integração Internacional da Lusofonia Afro-Brasileira, Redenção, Ceará, Brazil. The experimental design used was completely randomized in a split-plot scheme. The plots were five levels of electrical conductivity of irrigation water (ECw: 0.5, 1.5, 2.5, 3.5, and 4.5 dS m-1) and the subplots consisted of two formulations of substrates (SB1: sandy soil + sand + bovine manure; and SB2: sandy soil + sand + biochar; both in a 1:1:1 proportion on volume basis), with five repetitions of 25 seeds. The increase in the electrical conductivity of irrigation water from 1.5 dS m-1 reduces the percentage, speed index, and mean speed of emergence and increases the mean time of emergence of the watermelon crop. Substrate formulated with biochar reduces the time and increases the emergence indexes, besides enabling higher growth and biomass accumulation of watermelon seedlings. Salt stress reduces the growth, biomass, and quality of watermelon seedlings, but with less intensity with the addition of biochar.
Nutritional diagnosis of mango plants post-harvest in anticipation of pre-flowering avoids nutritional stress
Authors: Rezende Jefrejan S. , Freire Fernando J. , Silva Suellen R. V. da , Musser Rosimar dos S. , Cavalcante Ítalo H. L. , Saldanha Eduardo C. M. , Santos Renato L. dos , Cunha Jailson C.
Published: March 2023

ABSTRACT The São Francisco Valley region of Brazil is a leading exporter of mango fruits. Previous nutritional diagnosis can identify stresses, provide adjustments for nutritional limitations, and promote more efficient fertilization and nutrient management. This study aimed to compare the nutritional diagnosis of mango trees in the post-harvest and pre-flowering phases and to correlate them with productivity. Norms and indices of the Integrated System of Diagnosis and Recommendation (DRIS), as well as the Nutritional Balance Index and the Potential of Response to Fertilization were generated for each phenological phase of the mango trees. Optimal concentrations and ranges of nutrients were established, classified as deficient, balanced, or excessive, and subsequently compared to each other and to values recommended in the literature. The indices were correlated with the productivity of the orchards in each phenological phase of the mango trees. When comparing the DRIS norms of post-harvest and pre-flowering phenological phases, 55% of the averages differed. The sufficiency ranges of the post-harvest and pre-flowering phases were generally different from those referenced in the literature. Nutritional diagnoses for P, K, Ca, Mg, S, Zn, and Cl altered between phenological phases. The nutritional diagnosis performed in the post-harvest phase showed that nutritional imbalance affected productivity.