bluuebunny/crossref_metadata_2025 huggingface dataset:
High Expression Levels of the Long Non-Coding RNAs Lnc-IRF2-3 and Lnc-KIAA1755-4 Are Markers of Poor Prognosis in Chronic Lymphocytic Leukemia
Authors: Tosic Natasa , Tomic Vujovic Kristina , Vukovic Vojin , Kotur Nikola , Stankovic Biljana , Marjanovic Irena , Antic Darko , Sarac Sofija , Bibic Tamara , Ivanovic Jelena , Zukic Branka , Karan-Djurasevic Teodora
Published: January 2025

Long non-coding RNAs (lncRNAs) play complex roles at multiple levels of gene regulation, thus modulating key cellular processes involved in the pathogenesis and progression of cancer. Aberrant expression of lncRNAs has been reported in various malignancies, including chronic lymphocytic leukemia (CLL). We investigated the expression of lnc-IRF2-3 and lnc-KIAA1755-4 in peripheral blood mononuclear cells of 112 previously untreated CLL patients by quantitative reverse-transcriptase polymerase chain reaction. Both lncRNAs were found to be overexpressed in CLL samples in comparison to healthy controls, and their high levels were associated with adverse clinico-biological characteristics of patients at diagnosis. High lnc-IRF2-3 expression was associated with high leukocyte and lymphocyte counts, high β2-microglobulin, advanced Binet stage, unfavorable cytogenetics, CD38-positivity and IGHV-unmutated status. Regarding lnc-KIAA1755-4, its high expression was associated with high leukocyte count, lymphocyte count, β2-microglobulin, lactate dehydrogenase and low hemoglobin, as well as with IGHV-unmutated status. In addition, we observed shorter time to first treatment and overall survival of patients expressing high levels of both lncRNAs in comparison to low-expressing patients. In summary, our study showed that high lnc-IRF2-3 and lnc-KIAA1755-4 expression at diagnosis predicts poor survival in CLL. The mechanisms of their upregulation, as well as their specific targets in CLL cells, remain to be elucidated.
Melatonin Mitigates Acidosis-Induced Neuronal Damage by Up-Regulating Autophagy via the Transcription Factor EB
Authors: Shi Yan , Mi Zhaoyu , Zhao Wei , Hu Yue , Xiang Hui , Gan Yaoxue , Yuan Shishan
Published: January 2025

Acidosis, a common feature of cerebral ischemia and hypoxia, results in neuronal damage and death. This study aimed to investigate the protective effects and mechanisms of action of melatonin against acidosis-induced neuronal damage. SH-SY5Y cells were exposed to an acidic environment to simulate acidosis, and a photothrombotic (PT) infarction model was used to establish an animal model of cerebral ischemia of male C57/BL6J mice. Both in vivo and in vitro studies demonstrated that acidosis increased cytoplasmic transcription factor EB (TFEB) levels, reduced nuclear TFEB levels, and suppressed autophagy, as evidenced by elevated p62 levels, a higher LC3-II/LC3-I ratio, decreased synapse-associated proteins (PSD-95 and synaptophysin), and increased neuronal apoptosis. In contrast, melatonin promoted the nuclear translocation of TFEB, enhanced autophagy, and reversed neuronal apoptosis. Moreover, the role of TFEB in melatonin’s neuroprotective effects was validated by modulating TFEB nuclear translocation. In conclusion, melatonin mitigates acidosis-induced neuronal damage by promoting the nuclear translocation of TFEB, thereby enhancing autophagy. These findings offer new insights into potential treatments for acidosis.
Exogenous Melatonin Enhances Rice Blast Disease Resistance by Promoting Seedling Growth and Antioxidant Defense in Rice
Authors: Yuan Hongliang , Qian Jingya , Wang Chunwei , Shi Weixi , Chang Huiling , Yin Haojie , Xiao Yulin , Wang Yue , Li Qiang
Published: January 2025

In order to analyze the physiological regulation mechanisms associated with exogenous melatonin on rice blast, this study treated rice seedlings with different concentrations of melatonin (0, 20, 100, and 500 µmol/L) in order to investigate the growth characteristics, root morphology, superoxide dismutase (SOD) activity, peroxidase (POD) activity, catalase (CAT) activity, malondialdehyde (MDA) content, hydrogen peroxide (H2O2) content, and soluble protein content of rice seedlings. The results indicated that 100 µmol/L of melatonin exhibited a significant effect, improving the growth and antioxidant capacity of rice seedlings under rice blast fungus infection. The disease resistance level of rice seedlings against rice blast significantly decreased by 31.58% when compared to the 0 µmol/L melatonin treatment, while the plant height, stem base width, plant leaf area, total root length, aboveground dry weight, aboveground fresh weight, and underground fresh weight significantly increased by 8.72% to 91.38%. Treatment with 100 µmol/L of melatonin significantly increased catalase activities and soluble protein content, with respective increases of 94.99% and 31.14%. Simultaneously, the contents of malondialdehyde and hydrogen peroxide significantly decreased, reaching 18.65% and 38.87%, respectively. The gray relational grade analysis indicated that hydrogen peroxide content and resistance level exhibit the highest gray relational grades with melatonin concentration and, so, can be used to evaluate the effect of melatonin on the severity of rice blast fungus infection. Furthermore, the membership function analysis revealed that the 100 µmol/L melatonin treatment had the highest membership function value, indicating a significant improvement in the resistance of rice seedlings to rice blast disease. In conclusion, 100 µmol/L of melatonin enhances the resistance of rice seedlings to rice blast disease through promoting their growth and strengthening their antioxidant defenses. This study provides new insights into the tolerance mechanisms of rice seedlings against rice blast disease.
A Comprehensive Review of the Contribution of Mitochondrial DNA Mutations and Dysfunction in Polycystic Ovary Syndrome, Supported by Secondary Database Analysis
Authors: Kobayashi Hiroshi , Matsubara Sho , Yoshimoto Chiharu , Shigetomi Hiroshi , Imanaka Shogo
Published: January 2025

Polycystic ovary syndrome (PCOS) is a common endocrine disorder affecting women of reproductive age characterized by a spectrum of clinical, metabolic, reproductive, and psychological abnormalities. This syndrome is associated with significant long-term health risks, necessitating elucidation of its pathophysiology, early diagnosis, and comprehensive management strategies. Several contributory factors in PCOS, including androgen excess and insulin resistance, collectively enhance oxidative stress, which subsequently leads to mitochondrial dysfunction. However, the precise mechanisms through which oxidative stress induces mitochondrial dysfunction remain incompletely understood. Comprehensive searches of electronic databases were conducted to identify relevant studies published up to 30 September 2024. Mitochondria, the primary sites of reactive oxygen species (ROS) generation, play critical roles in energy metabolism and cellular homeostasis. Oxidative stress can inflict damage on components, including lipids, proteins, and DNA. Damage to mitochondrial DNA (mtDNA), which lacks efficient repair mechanisms, may result in mutations that impair mitochondrial function. Dysfunctional mitochondrial activity further amplifies ROS production, thereby perpetuating oxidative stress. These disruptions are implicated in the complications associated with the syndrome. Advances in genetic analysis technologies, including next-generation sequencing, have identified point mutations and deletions in mtDNA, drawing significant attention to their association with oxidative stress. Emerging data from mtDNA mutation analyses challenge conventional paradigms and provide new insights into the role of oxidative stress in mitochondrial dysfunction. We are rethinking the pathogenesis of PCOS based on these database analyses. In conclusion, this review explores the intricate relationship between oxidative stress, mtDNA mutations, and mitochondrial dysfunction, offers an updated perspective on the pathophysiology of PCOS, and outlines directions for future research.
Volatile Cues from Fresh Cattle Dung Can Drive Horn Fly Egg-Laying and Fecal Attraction to Horn Flies, Haematobia irritans (Diptera: Muscidae)
Authors: Espinoza Javier , Soto Isabel , Arriagada Joaquín , Lizama Marcelo , Aninao Nehuen , Aniñir Washington , Ungerfeld Emilio M. , Chacón-Fuentes Manuel , Quiroz Andrés
Published: January 2025

The horn fly is an economically important hematophagous ectoparasite of cattle. Its management relies heavily on broad-spectrum pesticides, which are harmful to the environment and have led to the development of resistance. Therefore, alternative control methods are needed. Semiochemicals involved in communication between horn flies and their host are a promising alternative. Considering that the egg-laying of this fly occurs almost exclusively in fresh cattle dung, and most parts of its life cycle occur totally in dung, dung volatiles might play an important role in horn fly behavior. Therefore, the main aim of this study was to evaluate the effects of volatile blends and compounds emitted from fresh/aged cattle dung on the olfactory response and oviposition of horn flies. Dung blends were captured and analyzed by SPME-GC/MS. p-Cresol was the most abundant compound in dung blends, followed by α- and β-pinene, limonene, and β-caryophyllene, among other common dung volatiles. Fresh-dung volatiles attracted males and females in a Y-tube olfactometer, and they elicited the egg-laying of flies in two-choice tests. p-Cresol and α-pinene were attractive to females and they elicited higher oviposition, demonstrating that dung volatile semiochemicals, in part, lend to dung attractiveness and stimulate the horn fly oviposition.
Morphogenetic Identification of a New Record Condica capensis (Lepidoptera: Noctuidae) in Yunnan, China
Authors: Qian Pengfan , Fan Jiayin , Zhang Xiaoyuan , Zeng Minfang , Han Xiaolong , Li Yonghe , Luo Xulu
Published: January 2025

Condica capensis (Lepidoptera: Noctuidae), a newly identified pest in Yunnan Province, China, poses a threat to safflower crops. Discovered in Nanhua County in November 2023, the pest damages safflower at multiple life stages, especially during its larval stage, when it feeds on leaves, tender stems, and flower filaments, sometimes causing the entire plant to die. Morphological and molecular analyses, including mitochondrial cytochrome C oxidase I (COI) gene sequencing, confirmed its identity as C. capensis, a new species record for Yunnan. The study also documented the pest’s life cycle, reproductive behavior, and natural enemies, highlighting the potential for biological control using parasitic wasps such as Cotesia sp. This research emphasizes the need for accurate pest identification and monitoring to develop effective, sustainable pest management strategies. As safflower cultivation grows in Yunnan, managing C. capensis is critical to safeguarding local agriculture and preventing broader agricultural threats.
The Association Between Temporomandibular Disorders and Tinnitus: Evidence and Therapeutic Perspectives from a Systematic Review
Authors: Dipalma Gianna , Inchingolo Alessio Danilo , Pezzolla Carmela , Sardano Roberta , Trilli Irma , Di Venere Daniela , Inchingolo Francesco , Palermo Andrea , Inchingolo Angelo Michele
Published: January 2025

Background/Objectives: Tinnitus, often described as a ringing in the ears, affects a significant portion of the population, varying in perception and severity. Methods: This systematic review investigates the correlation between tinnitus and temporomandibular joint disorders (TMDs) within a PRISMA-compliant framework, ensuring methodological transparency and rigor. Using databases, such as PubMed, Scopus, and Web of Science, we analyzed studies from the past decade to evaluate clinical and observational evidence. Results: A significant association between TMD and tinnitus was found, with somatosensory and neuroplastic mechanisms contributing to this relationship. Key therapeutic approaches identified include manual therapy and multidisciplinary treatments, demonstrating potential clinical efficacy. Conclusions: However, the available evidence remains inconsistent, emphasizing the need for further research with standardized methodologies to improve understanding and refine therapeutic strategies. This review provides a foundation for future studies aiming to enhance tinnitus management by addressing underlying TMD-related mechanisms.
The Potential Role of Nasal Cytology in Respiratory Diseases: Clinical Research and Future Perspectives
Authors: Marcuccio Giuseppina , Raffio Giuseppina , Ambrosino Pasquale , Candia Claudio , Cantone Elena , Detoraki Aikaterini , Maniscalco Mauro
Published: January 2025

Nasal cytology is a non-invasive, affordable, and easily executable technique commonly used in research to study rhinitis and, to a lesser extent, chronic rhinosinusitis. It is particularly useful for the differential diagnosis of non-allergic rhinitis and for phenotyping chronic rhinosinusitis. Allergic rhinitis, asthma, and aspirin intolerance are frequent comorbidities of chronic rhinosinusitis. A diagnostic system has been proposed to assess the severity of chronic rhinosinusitis (clinical-cytological grading), incorporating nasal cytology and comorbidity observation. This score correlates with the recurrence risk of chronic rhinosinusitis with nasal polyposis. Specifically, a higher grade is often linked to asthma, aspirin intolerance, a recurrent disease requiring surgery, and a mixed cell phenotype (eosinophilic and mast cell). Although nasal cytology has been shown to be able to replace bronchial analysis with acceptable precision due to its technical characteristics, its use in diseases affecting both upper and lower airways remains limited. The main limitation of this technique is its lack of standardization, which currently hinders its widespread clinical adoption despite its increasing familiarity among allergists and otolaryngologists. In the context of the unitary airways hypothesis, nasal cytology could also provide valuable insights for managing lower airway diseases like chronic obstructive pulmonary disease and obstructive sleep apnea syndrome, which significantly impact quality of life and healthcare costs. This review aims to provide an overview of nasal cytology, highlighting its limitations and potential applications in chronic respiratory diseases.
Renal Function and the Role of the Renin–Angiotensin–Aldosterone System (RAAS) in Normal Pregnancy and Pre-Eclampsia
Authors: Tsikouras Panagiotis , Nikolettos Konstantinos , Kotanidou Sonia , Kritsotaki Nektaria , Oikonomou Efthymios , Bothou Anastasia , Andreou Sotiris , Nalmpanti Theopi , Chalkia Kyriaki , Spanakis Vlasios , Tsikouras Nikolaos , Pagkaki Christina , Iatrakis George , Damaskos Christos , Garmpis Nikolaos , Machairiotis Nikolaos , Nikolettos Nikolaos
Published: January 2025

Objective: Pre-eclampsia (PE) is a complex, advancing condition marked either by the recent emergence of hypertension and proteinuria or by the recent onset of hypertension accompanied by notable end-organ impairment, which may occur with or without proteinuria. PE usually appears in the postpartum phase or after the 20th week of gestation, though it might appear sooner in cases of molar pregnancy. Pathophysiology: This disorder is a serious multisystem condition during pregnancy that can lead to serious, life-threatening complications for both the mother and the fetus if it is not diagnosed and managed promptly. These changes result from widespread and intense vasoconstriction caused by renin, angiotensin II (Ang II), aldosterone, and catecholamines. The renin–angiotensin–aldosterone system (RAAS) and its sequence of signaling reactions demonstrate its crucial role in regulating blood pressure and electrolyte balance that diverges in PE from that observed in healthy pregnancies. However, it is widely recognized that the above description is incomplete for pre-eclampsia and further relationships should be analyzed. The purpose of this article is to review and synthesize alterations in renal function and the RAAS during normal pregnancy and pre-eclampsia. We aim to provide clinical gynecologists with a comprehensive understanding of how pregnancy-specific adaptations can impact renal function and the RAAS, as well as the deviations observed in pre-eclampsia. Conclusion: This information is essential to enhance clinical awareness, improve the accuracy of diagnosis, and support evidence-based decision-making in the management of pregnant patients, especially those complicated by pre-eclampsia.
Application Prospects and Challenges of VHF Data Exchange System (VDES) in Smart Fisheries
Authors: Wu Zuli , Xiong Minsi , Cheng Tianfei , Dai Yang , Zhang Shengmao , Fan Wei , Cui Xuesen
Published: January 2025

Smart fisheries are an important way to promote the sustainable development of fisheries, and efficient and reliable marine communication systems are the key to realizing smart fisheries. As an emerging marine communication technology, the VHF Data Exchange System (VDES) has the advantages of a high data transmission rate, large communication capacity, and wide coverage, providing new opportunities for the transformation and upgrading of smart fisheries. This paper introduces the technical architecture and functions of the VDES, compares it with existing marine communication technologies, analyzes the key requirements of a smart fishery, and assesses how the VDES meets these requirements. The potential application scenarios of the VDES in smart fishery fields such as fishing vessel monitoring, fishery resource management, and maritime security are discussed. The challenges faced by the VDES in the application of smart fisheries, such as technology, policies and regulations, and construction cost layout, are analyzed, and its future development trend is prospected. Suggestions such as its integration with emerging technologies, the realization of global seamless coverage, and the strengthening of international cooperation and data sharing are proposed. This paper aims to provide theoretical guidance and scientific reference for the promotion and application of the VDES in smart fisheries.
The Significance of Renal Impairment in Children with Eating Disorders
Authors: Hamilton Avisha Meleika , Eisenhut Michael
Published: January 2025

Background: Eating disorders have previously been associated with renal impairment. Low muscle mass reduces serum creatinine used for the calculation of the estimated glomerular filtration rate (eGFR), leading to overestimation of renal function. To solve this problem, the development of a tool to detect renal impairment in individual patients with a specific muscle mass is required to individualize risk assessment for further management. The objectives of our study were as follows: To investigate the percentage drop in creatinine (pdCr) during rehydration as a new indicator of renal dysfunction not dependent on muscle mass and to investigate a correlation between cardiovascular function and fluid management with renal function. Methods: In a 5-year retrospective cohort study of all consecutive children admitted because of an eating disorder, renal function expressed as eGFR on admission and as pdCr between admission and the lowest creatinine level was analysed in relation to cardiovascular parameters and fluid management. Results: We included 29 patients. The mean age was 13.4 years. A pdCr after admission was noted in 26/29 (89.7%). The eGFR was <90 in 15 (65%) and improved to >90 in 13/15 (86.6%). In patients with a fluid management plan, there was a median of 18.6% for those with pdCr and 6.4% (p = 0.02) for those without. Renal dysfunction was not related to cardiovascular parameters. Conclusions: The majority of patients with an eating disorder had renal impairment. PdCr was more sensitive in the detection of renal impairment in individual patients compared to eGFR.
Enhancing the Valuation of Cultural Ecosystem Services Provided by Coastal Beaches Through Economic and Biophysical Methods
Authors: Oh Chi-Ok , Kim Namhee , Kang Yoonja
Published: January 2025

This study evaluates cultural ecosystem services (CESs) provided by coastal beaches in Korea using two valuation methods: choice experiments (CEs) and the energy method (EM). CESs, which include non-material benefits like recreation and esthetic enjoyment, are difficult to quantify through traditional market-based approaches. The CE method estimates individuals’ willingness to pay (WTP), offering an economic valuation, while the EM calculates the energy inputs required to sustain CESs, providing a biophysical perspective. The study compares CES values from both methods across 266 beaches in Korea. CES values from the CE method ranged from 6 million Korean won (KRW) (USD 5400) to KRW 93 billion (USD 84 million), while the EM yielded values between KRW 40 million (USD 36,000) and KRW 112 billion (USD 101 million). The EM generally yields higher valuations, particularly for rural beaches with fewer visitors, where ecological contributions dominate. In contrast, the CE values are closely aligned with the EM results for urban beaches, where human preferences and ecological inputs converge. These findings highlight the value of integrating economic and biophysical perspectives to provide policymakers with comprehensive data for balancing economic development and ecological conservation in coastal management. By adopting a dual-method approach, this research provides policymakers with concrete insights into optimizing resource allocation, prioritizing conservation efforts for less-visited rural beaches, and supporting sustainable coastal development tailored to regional characteristics.
Reliability and Validity of Key Performance Metrics of Modified 505 Test
Authors: Živković Aleksandar , Marković Srdjan , Cuk Ivan , Knežević Olivera M. , Mirkov Dragan M.
Published: January 2025

This study aims to explore the reliability and validity of essential performance metrics derived from a modified 505 Change of Direction (CoD) test. Twenty-seven physically active male participants (age: 18.77 ± 1.73 years) who engaged in three to five training sessions per week were assessed using both standard and modified sprint and CoD protocols. The evaluation focused on sprint time, CoD time, total time, and the Limb Symmetry Index (LSI). The modified protocols demonstrated good reliability for sprint and CoD times (ICC > 0.8, CV < 3%) and high validity, with strong correlations between standard and modified tests for sprint (r = 0.59–0.80) and CoD times (r = 0.58–0.80). The total time metric showed excellent reliability (ICC > 0.9), supporting its utility as a comprehensive measure. Reaction-time inclusion increased variability (CV > 7%) but enhanced ecological validity by reflecting real-world conditions. However, the LSI exhibited lower reliability (ICC < 0.6), indicating the need for further refinement. Combining multiple performance measures, the modified protocols streamlined assessments, reducing fatigue and redundancy without compromising precision. These findings highlight the potential of integrating multidimensional agility metrics in athletic evaluations, bridging the gap between controlled testing and competitive demands. Future research should explore broader populations and sport-specific stimuli to enhance applicability.
A Method for Calculating Small Sizes of Volumes in Postsurgical Thyroid SPECT/CT Imaging
Authors: Ttofi Elena , Kyriacou Costas , Leontiou Theodoros , Parpottas Yiannis
Published: January 2025

Differentiated thyroid cancer treatment typically involves the surgical removal of the whole or largest part of the thyroid gland. Diagnostic procedures are useful both before and after treatment to determine the need for radioiodine ablation, re-stage the disease, monitor disease progression, or evaluate treatment efficacy. SPECT/CT imaging can be utilized to identify small, distant iodine-avid metastatic lesions and assess their uptake and volume for the above purposes as well as for performing lesion-based dosimetry when indicated. The objective of this study was to develop and validate a method for calculating small sizes of volumes in SPECT/CT imaging as well as to perform calculations utilizing I-131 and I-123 postsurgical SPECT/CT images from a neck–thyroid phantom. In this approach, the calculated volume was unaffected by radiation spillover from high-uptake voxels since it was the result from the successive application of the gray-level histogram technique to SPECT and CT 3D matrices. Beforehand, the SPECT 3D matrix was resized and aligned to the corresponding CT one. The method was validated following the clinical protocols for postsurgical thyroid imaging by using I-123 and I-131 scatter and attenuation-corrected SPECT/CT images from a neck–thyroid phantom. The phantom could accommodate two volumes of different sizes (0.5, 1, 1.5, 3, and 10 mL) and enclose anatomical tissue-equivalent main scattering structures. For the 0.5 and 10 mL volumes, the % differences between the actual and the calculated volumes were 15.2% and 1.2%, respectively. Radiation spillover was only present in SPECT images, and it was more profound at higher administered activities, in I-131 than in I-123 images, and in smaller volumes. When SPECT/low-dose-CT imaging is performed, this method is capable of accurately calculating small volumes without the need of additional modalities.
Synthesis, Stability, and Tribological Performance of TiO2 Nanomaterials for Advanced Applications
Authors: Zeng Kai , Cheng Liang , Hu Wenjing , Li Jiusheng
Published: January 2025

The enhancement of tribological properties represents a pivotal strategy for achieving energy efficiency and environmental protection. Titanium dioxide (TiO2) nanomaterials have been garnering significant attention due to their exemplary tribological properties and due to the abundance of titanium reserves. The present review is concerned with the study of TiO2 nanomaterials in lubricants. The properties and various synthesis methods of TiO2 nanomaterials are presented. The dispersion stability of these TiO2 nanomaterials in lubricating oils is discussed in depth, as well as strategies to improve their dispersion stability, such as enhancing compatibility with base oils, reducing the dynamic light scattering (DLS) particle size, modulating the zeta potential, and optimizing the drying step. Aggregation and dispersion instability remain key challenges for TiO2 nanomaterials, especially bare TiO2 nanoparticles (NPs). In contrast, in situ surface-modified TiO2 NPs show improved stability and tribological performance, offering promise for further research. The tribological performance of lubricants has been demonstrated to be enhanced by TiO2 nanomaterials, with the observed enhancement attributed to the synergistic effect of multiple mechanisms, including rolling, patching, polishing, and the formation of a protective film. Furthermore, future research suggestions are proposed to provide a reference for the design and synthesis of high-performance TiO2 nano-lubricants and promote their wide application.
Investigation of the Contact Characteristics of a Single-Nut Ball Screw Considering Geometric Errors
Authors: Liu Jun , Zhou Huaxi , Wang Xiaoyi , Zhou Changguang
Published: January 2025

As the critical performance index of ball screws, the contact characteristics have a significant influence on the lubricant properties, tribological properties, and wear properties of ball screws, which further directly affect the service life of ball screws. The non-uniform load distribution induced by geometric errors results in imbalances among balls along the nut, negatively impacting the service life of ball screws. This study focuses on the load distribution of single-nut ball screws under low-speed working conditions. This paper proposes a self-adjustable model of load distribution that considers the flexibility of the screw and nut with respect to the determination of the non-bearing ball. A refined model for axial stiffness is proposed to systematically analyze the influence of geometric errors on stiffness variations under various loading conditions. The results confirm the ability of the proposed model to reveal the static load distribution in view of geometric errors. The greatest discrepancy observed between the theoretical predictions and the experimental data was 9.22%. The numerical simulations demonstrate variation trends in the normal contact load, the loaded-ball number, and the axial deformation of a nut with geometric errors. Furthermore, the relationship between the axial stiffness of a single-nut ball screw and the geometric error is obtained. The self-adjustable model of load distribution is helpful for studying the carrying capacity of a single-nut ball screw. The findings of the study provide a definite reference for optimization of structural design and wear life prediction.
Compressive Properties of Composite Sandwich Structure with Fractal Tree-Inspired Lattice Core
Authors: Han Jian , Ma Xin , Yang Rui , Sun Shiyong
Published: January 2025

A novel sandwich structure of a fractal tree-like lattice (SSFL) is proposed. The geometry characteristics were constructed based on the fractal tree-like patterns found in many biological structures, such as giant water lilies and dragon blood trees. The compressive performance of the proposed structures with different fractal orders was experimentally and numerically investigated. The experimental samples were made by 3D printing technology. Axial compression tests were conducted to study the compressive performance and failure mode of the SSFLs. The results indicated that the new structure was good at multiple bearing and energy absorption. The finite element method (FEM) was performed to investigate the influence of geometry parameters on the compression behaviors of the SSFLs. The findings of this study provide an effective guide for using the fractal method to design lattice structures with a high bearing capacity.
Design of Tool Shape and Evaluation of Deformation Behavior by Digital Image Correlation Method in V-Bending of Sheet Metal Using Plastic Tools Manufactured by 3D Printer
Authors: Nakamura Naotaka , Hata Yuri , Daodon Witthaya , Ikeda Daiki , Adachi Nozomu , Todaka Yoshikazu , Abe Yohei
Published: January 2025

In the V-bending of sheet metals using a pair of plastic punch and die manufactured by a 3D printer, the effects of two different dimensions designed with the same tool geometry on the deformation behaviors of the punch, die, and sheet were evaluated. The deformation behavior and strain distribution of the punch, die, and sheet were analyzed using a digital image correlation method. Sheets from pure aluminum to ultra-high-strength steel were bent using the two tools with different spans; one was designed on the assumption of tool steel material, and the other was designed on the assumption of plastic material. In both tools, the large compressive strain appeared around the center of the punch tip and on the corners of the die. The tools with a long span for the plastic material gave a lower bending force and small deformation of the plastic tools. The angle difference between a bent sheet at the bottom dead center and a tool was smaller for the tools with the long span, although the springback in the bent sheet appeared. It was found that the design method on the assumption of the plastic material is effective for the V-bending plastic tools.
Research on the Correlation Between the Chemical Components and the Macroscopic Properties of Asphalt Binder
Authors: Li Zhihao , Cao Xuejuan , Li Jue , Yang Xiaoyu
Published: January 2025

The chemical composition of asphalt binder is closely related to its macroscopic properties, and as an important road building material, its performance directly affects the service performance of asphalt binder pavement. Saturate, aromatic, resin, and asphaltene are the four most common chemical components of asphalt binders, collectively known as the SARA components. The SARA components are used to establish the corresponding relationship between the chemical composition and the macroscopic properties of asphalt binder, which is of great significance for further research on and development of high-performance asphalt pavement materials. This study used eight types of virgin asphalt binders as raw materials, labeled A–H. Firstly, the thin-layer chromatography–flame ionization detection (TLC-FID) method was used to test the SARA contents of the different asphalt binders. Then, the conventional, rheological, and low-temperature properties of the different binders were tested. Finally, gray relational analysis (GRA) and Pearson correlation analysis (PCA) were used to study the correlation between the asphalt binder’s SARA content and its macroscopic properties. The results indicate that the contents of asphaltenes and resins are crucial in determining the high-temperature performance of asphalt binder. By adjusting the ratio of these components, the high-temperature performance of asphalt binder can be optimized. An increase in the content of heavy components, particularly asphaltenes, negatively affects the low-temperature performance of asphalt binder. In contrast, a higher aromatic content enhances its low-temperature performance.
The Influence of Hot Isostatic Pressing on the Mechanical Properties of Ti-6Al-4V Samples Printed Using the LENS Method
Authors: Gzik-Zroska Bożena , Joszko Kamil , Piątek Agata , Wolański Wojciech , Kawlewska Edyta , Szarek Arkadiusz , Kajzer Wojciech , Stradomski Grzegorz
Published: January 2025

The aim of this work was to assess the influence of the parameters of the hot isostatic pressing (HIP) process and the direction of printing of Ti-6Al-4V samples made using the laser-engineered net shaping (LENS) method on strength properties. The tests were carried out using a static testing machine and a digital image correlation system. Samples before and after the HIP process were tested. The HIP process was carried out at a temperature of 1150 °C, a heating time of 240 min and various pressure values of 500, 1000 and 1500 bar. Based on the comparative analysis of the test results, it has been shown that the ability to adjust the parameters of the HIP process has a significant impact on the final mechanical properties of the samples.
Unlocking the Potential of Ti3C2Tx MXene: Present Trends and Future Developments of Gas Sensing
Authors: Teli Aviraj M. , Mane Sagar M. , Mishra Rajneesh Kumar , Jeon Wookhee , Shin Jae Cheol
Published: January 2025

In recent years, the need for future developments in sensor technology has arisen out of the changing landscape, such as pollution monitoring, industrial safety, and healthcare. MXenes, a 2D class of transition metal carbides, nitrides, and carbonitrides, have emerged as a particularly promising group in part due to their exceptionally high conductivity, large area, and tunable surface chemistry. Proposed future research directions, including material modification and novel sensor designs, are presented to maximize Ti3C2Tx MXene-based sensors for various gas sensing applications. While recent progress in Ti3C2Tx MXene-based gas sensors is reviewed, we consolidate their material properties, fabrication strategy, and sensing mechanisms. Further, the significant progress on the synthesis and applications of Ti3C2Tx MXene-based gas sensors, as well as the innovative technologies developed, will be discussed in detail. Interestingly, the high sensitivity, selectivity, and quick response times identified in recent studies are discussed, with specificity and composite formation highlighted to have a significant influence on sensor performance. In addition, this review highlights the limitations witnessed in real-life implementability, including stability, the possibility of achieving reproducible results, and interaction with currently available technologies. Prospects for further work are considered, emphasizing increased production scale, new techniques for synthesis, and new application areas for Ti3C2Tx MXenes, including electronic nose and environmental sensing. Contemplating the existing works, further directions and the development framework for Ti3C2Tx MXene-based gas sensors are discussed.
Development of an Engineered Bacterial Endophyte: Promoting Plant Growth Through Pyrroloquinoline Quinone (PQQ) Synthesis
Authors: Fang Ti , Lo Shou-Chen , Yu Yu-Ning , Sou Nga-Lai , Hung Shih-Hsun Walter , Peng Jian-Hau , Chiang En-Pei Isabel , Huang Chieh-Chen
Published: January 2025

Endophytic bacteria are a group of microorganisms that can intercellularly colonize plant hosts without causing apparent damage or disease. Our previous works found that a pyrroloquinoline quinone (PQQ)-producing endophyte could promote plant growth and systemic tolerance. To demonstrate this PQQ-producing endophyte’s beneficial role in plants, a set of five PQQ synthesis genes from Gluconobacter oxydans was introduced into both Escherichia coli JM109 and Bacillus subtilis RM125, a BsuM-deficient mutant of laboratory strain B. subtilis 168. Interestingly, both strains harboring the PQQ synthesis genes exhibited significantly higher optimal optical density than control strains. In a carbon flux analysis, both strains showed a noticeable increase in their citric acid, alpha-ketoglutaric acid, and succinic acid levels. Conversely, in E. coli, pyruvic acid, malic acid, and fumaric acid levels decreased. These results suggest that PQQ impacts various host species differently. Furthermore, the presence of PQQ in fermentation broth was also confirmed in the RM125 PQQ synthesis recombinant strain. Subsequent experiments by inoculating those Bacillus strains revealed that the laboratory host strain could function as an endophyte, and the PQQ transgenic strain could further promote the growth of Arabidopsis thaliana and increase the number of siliques. These findings confirm PQQ’s vital role in endophyte-mediated plant growth promotion and also suggest the potential of B. subtilis transformed with PQQ genes as an engineered endophyte for studying PQQ’s biological functions in plants. This research is a step forward in understanding how specific substances can beneficially influence plant growth and systemic tolerance through endophytic mechanisms.
Exploring the Phytochemical Diversity and Anti-Plasmodial Potential of Artemisia annua and Artemisia afra from Different Geographical Locations in Cameroon
Authors: Shinyuy Lahngong M. , Loe Gisèle E. , Jansen Olivia , Ledoux Allison , Palmaerts Benjamin , Mamede Lúcia , Boussif Naima , Bonnet Olivier , Enone Bertin S. , Noukimi Sandra F. , Nchang Abenwie S. , Demeyer Kristiaan , Robert Annie , Ghogomu Stephen M. , Souopgui Jacob , Hallot Eric , Frederich Michel
Published: January 2025

In Cameroon, like in other African countries, infusions of Artemisia afra and Artemisia annua are widely used for the management of health-related problems, including malaria. The secondary metabolite contents of medicinal plants vary between different geographical regions and seasons, directly influencing their effectiveness in treating ailments. This study explores the phytochemical diversity and anti-plasmodial potential of A. annua and A. afra from distinct geographical locations within Cameroon, aiming to define the optimal chemical composition in terms of anti-plasmodial activity. Extracts were prepared from plants collected from diverse regions in Cameroon during both the rainy and dry seasons, and their metabolic contents were analyzed using Thin-Layer Chromatography (TLC), High Performance Liquid Chromatography (HPLC), and Gas Chromatography (GC). Their anti-plasmodial potential was assessed on a chloroquine-sensitive 3D7 Plasmodium falciparum strain. Additionally, the environmental parameters of the collecting sites were retrieved from multispectral satellite imagery. The activity profiles of the samples were associated with their environment, with distinct phytochemical compositions observed for each sample based on its geographical origin and season. Traces of artemisinin were detected in some of the A. afra samples, but it was present in the A. annua samples at a significantly higher concentration, especially in the rainy season samples (highest concentration in the Adamawa region, at 8.9% m/m artemisinin in the dry extract). Both plants are active at different levels, with A. annua more active due to the presence of artemisinin and A. afra probably active due to the presence of polyphenols. Both season and geographical location influence both plants’ metabolic contents and hence their antimalaria activity. These findings suggest that the selection of a suitable Artemisia sample for use as a potential antimalarial treatment should take into consideration its geographical origin and the period of collection.
Defect-Tolerant Memristor Crossbar Circuits for Local Learning Neural Networks
Authors: Oh Seokjin , Yoon Rina , Min Kyeong-Sik
Published: January 2025

Local learning algorithms, such as Equilibrium Propagation (EP), have emerged as alternatives to global learning methods like backpropagation for training neural networks. EP offers the potential for more energy-efficient hardware implementation by utilizing only local neuron information for weight updates. However, the practical implementation of EP using memristor-based circuits has significant challenges due to the immature fabrication processes of memristors, resulting in defects and variability issues. Previous implementations of EP with memristor crossbars use two separate circuits for the free and nudge phases. This approach can suffer differences in defects and variability between the two circuits, potentially leading to significant performance degradation. To overcome these limitations, in this paper, we propose a novel time-multiplexing technique that combines the free and nudge phases into a single memristor circuit. Our proposed scheme integrates the dynamic equations of the free and nudge phases into one circuit, allowing defects and variability compensation during the training. Simulations using the MNIST dataset demonstrate that our approach maintains a 92% recognition rate even with a 10% defect rate in memristors, compared to 33% for the previous scheme. Furthermore, the proposed circuit reduces area overhead for both the memristor circuit solving EP’s algorithm and the weight-update control circuit.
Molecular Dynamics Simulation of Low-Cycle Fatigue Behavior of Single/Polycrystalline Iron
Authors: Zhang Tianyu , Zhou Jinjie , Shen Jinchuan
Published: January 2025

The fatigue plastic mechanism and dislocation characteristics of engineering materials are the key to studying fatigue damage. In this study, the molecular dynamics (MD) method was employed to investigate the microstructural characteristics and fatigue mechanical properties of both single-crystalline and polycrystalline iron under varying strain amplitudes associated with cyclic hardening, cyclic softening, and cyclic saturation. The occurrence, accumulation, and formation process of the local plastic fatigue damage of monocrystalline/polycrystalline iron under fatigue load are discussed. The local plastic initiation and accumulation of single-crystal iron occur at the intersection of slip planes, which is the dislocation source. The 1/2<111> dislocation plays an important role in the fatigue plastic accumulation of single-crystal iron. Polycrystalline iron undergoes grain rotation and coalescence during cyclic loading. The grain size responsible for plastic deformation gradually increases. The initiation and accumulation of local plasticity occurs at the grain boundary, which eventually leads to fatigue damage at the grain boundary.
Excessive Iodine Intake During Lactation Is Not Related to the Incidence of Thyroid Disease: A 3-Year Follow-Up Study
Authors: Park Seon-Joo , Lee Do-Kyung , Lee Hae-Jeung
Published: January 2025

Objectives: This study aimed to investigate the relationship between excessive postpartum iodine intake and the incidence of thyroid disease in mothers, as well as child growth and development. Methods: Of 1054 participants in the 2019 nationwide survey that assessed maternal postpartum iodine intake, 684 mothers participated in a follow-up study. Data on maternal thyroid disease incidence and child growth and development from infant or toddler health checkups were collected. Iodine and nutrient intake were assessed using three-day dietary records, and serum thyroid hormones (triiodothyronine (T3), thyroid-stimulating hormone (TSH), and free thyroxine (free T4)) were measured. Relative risks (RRs) were estimated using Poisson regression analysis. Results: Among the 684 participants, 23 (3.4%) were diagnosed with thyroid disease by a physician during the follow-up period. The incidence of maternal thyroid disease was not significantly associated with excessive iodine intake, even after adjusting for confounding factors. Additionally, excessive maternal iodine intake was not related to subclinical hypothyroidism in mothers or child growth and development. Conclusions: After a three-year follow-up, no relationship was observed between high postpartum iodine intake and the risk of thyroid disease. Large-scale longitudinal studies are required to evaluate the long-term effects of excessive postpartum iodine intake on maternal health and child growth and development.
Postprandial Hypotension in Adults: Exploring Insulin Dynamics During a Mixed Meal Test
Authors: Karakaya Rahime Evra , Tam Abbas Ali , Fakı Sevgül , Karaahmetli Gülsüm , Özdemir Didem , Ersoy Reyhan , Topaloğlu Oya
Published: January 2025

Background/Objectives: Postprandial hypotension (PPH) is an important clinical condition in patients presenting with postprandial symptoms. The aims of this study were to determine the prevalence of PPH in patients with postprandial symptoms and to investigate the relationship between PPH and insulin, particularly in healthy adults. Methods: This study was conducted with 111 adult patients who were admitted to the clinic due to postprandial symptoms. Patients underwent the mixed meal test (MMT). Blood glucose, insulin, and C-peptide levels were measured at 0, 30, 60, 90, 120, 180, 240, and 300 min along with systolic blood pressure (sBP), diastolic blood pressure (dBP), and heart rate measurements during the MMT. Results: Serum adrenocorticotropic hormone (ACTH) levels were similar (p > 0.05), and cortisol levels were found to be higher in individuals without PPH compared to those with PPH before the MMT (p = 0.014). During the MMT, 23 patients (23.2%) had PPH. At the beginning of the test, serum glucose, insulin, C-peptide, and heart rate values were similar in patients with and without PPH; however sBP and dBP were significantly higher in the PPH group (p = 0.002 and p = 0.010, respectively). No correlation was found between sBP and insulin, glucose, and C-peptide at any time during the MMT except for a moderately significant positive correlation between glucose and sBP at 90 min in patients with PPH (r = 0.490, p = 0.018). A moderately negative correlation was found between the magnitude of sBP fall between 30 and 60 min and insulin and C-peptide levels in people with PPH (r = −0.420, p = 0.046; r = −0.564, p = 0.005; respectively). However, no significant relationships were observed between the magnitude of sBP fall at other time points and blood parameters (p > 0.05). Conclusions: A significant portion of adults with postprandial symptoms might have PPH, contributing to these symptoms. The lack of a relationship between insulin and glucose suggests that other physiological mechanisms beyond insulin and glucose may play a role in the pathogenesis of PPH in healthy individuals. Therefore, further research is needed to better understand the underlying causes of PPH.