bluuebunny/crossref_metadata_2025 huggingface dataset:
What Is It Like to Make a Prototype? Practitioner Reflections on the Intersection of User Experience and Digital Humanities/Social Sciences during the Design and Delivery of the “Getting to Mount Resilience” Prototype
Authors: Lee Ashlin
Published: August 2023

The digital humanities and social sciences are critical for addressing societal challenges such as climate change and disaster risk reduction. One way in which the digital humanities and social sciences add value, particularly in an increasingly digitised society, is by engaging different communities through digital services and products. Alongside this observation, the field of user experience (UX) has also become popular in industrial settings. UX specifically concerns designing and developing digital products and solutions, and, while it is popular in business and other academic domains, there is disquiet in the digital humanities/social sciences towards UX and a general lack of engagement. This paper shares the reflections and insights of a digital humanities/social science practitioner working on a UX project to build a prototype demonstrator for disaster risk reduction. Insights come from formal developmental and participatory evaluation activities, as well as qualitative self-reflection. The paper identifies lessons learnt, noting challenges experienced—including feelings of uncertainty and platform dependency—and reflects on the hesitancy practitioners may have and potential barriers in participation between UX and the digital humanities/social science. It concludes that digital humanities/social science practitioners have few skill barriers and offer a valued perspective, but unclear opportunities for critical engagement may present a barrier.
Quantification and Reduction of Uncertainty in Seismic Resilience Assessment for a Roadway Network
Authors: Jonnalagadda Vishnupriya , Lee Ji Yun , Zhao Jie , Ghasemi Seyed Hooman
Published: August 2023

The nation’s transportation systems are complex and are some of the highest valued and largest public assets in the United States. As a result of repeated natural hazards and their significant impact on transportation functionality and the socioeconomic health of communities, transportation resilience has gained increasing attention in recent years. Previous studies on transportation resilience have heavily emphasized network functionality during and/or following a scenario hazard event by implicitly assuming that sufficient knowledge of structural capacity and environmental/service conditions is available at the time of an extreme event. However, such assumptions often fail to consider uncertainties that arise when an extreme hazard event occurs in the future. Thus, it is essential to quantify and reduce uncertainties to better prepare for extreme events and accurately assess transportation resilience. To this end, this paper proposes a dynamic Bayesian network-based resilience assessment model for a large-scale roadway network that can explicitly quantify uncertainties in all phases of the assessment and investigate the role of inspection and monitoring programs in uncertainty reduction. Specifically, the significance of data reliability is investigated through a sensitivity analysis, where various sets of data having different reliabilities are used in updating system resilience. To evaluate the effectiveness of the model, a benchmark problem involving a highway network in South Carolina, USA is utilized, showcasing the systematic quantification and reduction of uncertainties in the proposed model. The benchmark problem result shows that incorporating monitoring and inspection data on important variables could improve the accuracy of predicting the seismic resilience of the network. It also suggests the need to consider equipment reliability when designing monitoring and inspection programs. With the recent development of a wide range of monitoring and inspection techniques, including nondestructive testing, health monitoring equipment, satellite imagery, LiDAR, etc., these findings can be useful in assisting transportation managers in identifying necessary equipment reliability levels and prioritizing inspection and monitoring efforts.
Analyzing Wind Effects on Long-Span Bridges: A Viable Numerical Modelling Methodology Using OpenFOAM for Industrial Applications
Authors: Zhang Yuxiang , MacReamoinn Reamonn , Cardiff Philip , Keenahan Jennifer
Published: August 2023

Aerodynamic performance is of critical importance to the design of long-span bridges. Computational fluid dynamics (CFD) modelling offers bridge designers an opportunity to investigate aerodynamic performance for long-span bridges during the design phase as well as during operation of the bridge. It offers distinct advantages when compared with the current standard practice of wind tunnel testing, which can have several limitations. The proposed revisions to the Eurocodes offer CFD as a methodology for wind analysis of bridges. Practicing engineers have long sought a computationally affordable, viable, and robust framework for industrial applications of using CFD to examine wind effects on long-span bridges. To address this gap in the literature and guidance, this paper explicitly presents a framework and demonstrates a workflow of analyzing wind effects on long-span bridges using open-source software, namely FreeCAD, OpenFOAM, and ParaView. Example cases are presented, and detailed configurations and general guidance are discussed during each step. A summary is provided of the validation of this methodology with field data collected from the structural health monitoring (SHM) systems of two long-span bridges.
The Synthesis and Crystal Structure of Six Quaternary Lithium-Alkaline Earth Metal Alumo-Silicides and Alumo-Germanides, A2LiAlTt2 (A = Mg, Ca, Sr, Ba; Tt = Si, Ge)
Authors: Kontomaris Paraskevi , Darone Gregory M. , Paredes-Quevedo Laura C. , Bobev Svilen
Published: August 2023

Reported are the synthesis and structural characterization of a series of quaternary lithium-alkaline earth metal alumo-silicides and alumo-germanides with the base formula A2LiAlTt2 (A = Ca, Sr, Ba; Tt = Si, Ge). To synthesize each compound, a mixture of the elements with the desired stoichiometric ratio was loaded into a niobium tube, arc welded shut, enclosed in a silica tube under vacuum, and heated in a tube furnace. Each sample was analyzed by powder and single-crystal X-ray diffraction, and the crystal structure of each compound was confirmed and refined from single-crystal X-ray diffraction data. The structures, despite the identical chemical formulae, are different, largely dependent on the nature of the alkaline earth metal. The differing cation determines the structure type—the calcium compounds are part of the TiNiSi family with the Pnma space group, the strontium compounds are isostructural with Na2LiAlP2 with the Cmce space group, and the barium compounds crystallize with the PbFCl structure type in the P4/nmm space group. The anion (silicon or germanium) only impacts the size of the unit cell, with the silicides having smaller unit cell volumes than the germanides.
Impact of Comorbidities and Previous Surgery on Mid-Term Results of Revision Total Knee Arthroplasty for Periprosthetic Joint Infection
Authors: Koch Kevin-Arno , Spranz David M. , Westhauser Fabian , Bruckner Tom , Lehner Burkhard , Alvand Abtin , Merle Christian , Walker Tilman
Published: August 2023

(1) Background: In the treatment of periprosthetic joint infection (PJI), the individual host status and previous surgical procedures appear to have a relevant influence on success rates and clinical outcome of knee revision surgery. Current data about the predictive value are limited in this subgroup of patients. (2) Methods: Retrospectively, 107 patients (109 knees) undergoing two-stage exchange knee arthroplasty for PJI using a rotating-hinge design with at least two years follow-up. The cumulative incidence (CI) for different endpoints was estimated with death as competing risk. Univariate and multivariate analyses for potential predictive factors were performed. Patient-related outcome measures (PROMs) for clinical outcome were evaluated. (3) Results: At 8 years, the CI of any revision was 29.6%, and of any reoperation was 38.9%. Significant predictors for risk of re-revision were the Charlson Comorbidity Index (CCI) and the number of previous surgical procedures prior to explanation of the infected implant. The functional and clinical outcome demonstrated acceptable results in the present cohort with a high comorbidity level. (4) Conclusions: A compromised host status and multiple previous surgical procedures were identified as negative predictors for re-revision knee surgery in the treatment of PJI. Reinfection remained the major reason for re-revision. Overall mortality was high.
Gastroesophageal Reflux Disease in Obesity: Bariatric Surgery as Both the Cause and the Cure in the Morbidly Obese Population
Authors: Masood Muaaz , Low Donald , Deal Shanley B. , Kozarek Richard A.
Published: August 2023

Gastrointestinal reflux disease (GERD) is a chronic, highly prevalent condition in the United States. GERD can significantly impact quality of life and lead to complications including aspiration pneumonia, esophageal stricture, Barrett’s esophagus (BE) and esophageal cancer. Obesity is a risk factor for GERD, which often improves with weight loss and bariatric surgery. Though the incidence of bariatric surgery, in particular, minimally invasive sleeve gastrectomy, has risen in recent years, emerging data has revealed that the severity or new onset of GERD may follow bariatric surgery. We performed a literature review to provide a detailed analysis of GERD with an emphasis on bariatric surgery as both the cure and the cause for GERD in the morbidly obese population. We also describe the pathophysiological mechanisms, management approach and treatment strategies of GERD following bariatric surgery.
Feasibility and Short-Term SpO2/FiO2 Changes in Hospitalized Adults with COVID-19 Pneumonia after Chest Physiotherapy with Threshold PEP Valve: A Cross-Sectional Study
Authors: Estela Júlia , Sánchez Emilio José , Mateu Georgina , Fernández Elena , Robert Eva , Pozo Silvia , Noray Mariona , Oliva Joan C. , Caballero Fernanda M. , Luján Manel
Published: August 2023

Background: One of the main features of COVID-19 pneumonia is hypoxemic acute respiratory failure (ARF), often requiring ventilatory support. The influence of chest physiotherapy in patients with ARF is not extensively studied. The aim of the study was to analyze the short-time effects of chest physiotherapy using a 10 cm H2O threshold valve in patients with COVID-19 and ARF. Methods; Quasi-experimental cross-sectional study, in hospitalized patients from March to May 2020. The fractions of inspired oxygen, oxygen saturation, heart rate, respiratory rate and dyspnea were collected before and after the starting session (day 1) and after the 5th day of therapy. Results: The final sample size included 125 patients. Significant differences (p < 0.01) were found in the pre-post intervention SpO2/FiO2 ratio (250 ± 88.4 vs. 275.6 ± 97.5, p < 0.001), reaching 354.4 ± 110.2 after 5 days of therapy (p < 0.001 with respect to the baseline). The respiratory and heart rate dyspnea level did not change during the intervention. In patients needing FiO2 > 0.4, the SpO2/FiO2 ratio improvement was higher than in patients with milder severity (46.85 ± 77.69, p < 0.01). Conclusions: Chest physiotherapy with a 10 cm H2O threshold valve seems to be a safe and tolerated intervention with short-term improvement in oxygenation in patients with COVID-19 pneumonia.
A Predictive Model of Major Postoperative Respiratory Adverse Events in Pediatric Patients Undergoing Rigid Bronchoscopy for Exploration and Foreign Body Removal
Authors: Yi Xiuwen , Ni Wenwen , Han Yuan , Li Wenxian
Published: August 2023

Background: No nomogram has been established to predict the incidence of major postoperative respiratory adverse events (mPRAEs) in children undergoing rigid bronchoscopy for airway foreign bodies (AFB) removal and exploration of the airway, though some studies have confirmed the risk factors. Methods: 1214 pediatric patients (≤3 years old) undergoing rigid bronchoscopy for AFB from June 2014 to December 2020 were enrolled in this study. The primary outcome was the occurrence of mPRAEs, including laryngospasm and bronchospasm. Following that, a nomogram prediction model for the mPRAEs was developed. Results: The incidence of mPRAEs was 84 (6.9%) among 1214 subjects. American Society of Anesthesiologists physical status (ASA-PS), intraoperative desaturation (SpO2 < 90%), procedural duration and ventilatory approach were all independent risk factors of mPRAEs. The area under the receiver operating characteristic curve (AUC) value of the nomogram for predicting mPRAEs was 0.815 (95% CI: 0.770–0.861), and the average AUC for ten-fold cross-validation was 0.799. These nomograms were well calibrated by Hosmer-Lemshow (p = 0.607). Decision curve analysis showed that the nomogram prediction model is effective in clinical settings. Conclusions: Combining ASA-PS, intraoperative desaturation, procedural duration, and ventilatory approach, the nomogram model is adequate for predicting the risk of developing mPRAEs, followed by rigid bronchoscopy for AFB removal and exploration.
Extracorporeal Membrane Oxygenation (VA-ECMO) in Management of Cardiogenic Shock
Authors: Koziol Klaudia J. , Isath Ameesh , Rao Shiavax , Gregory Vasiliki , Ohira Suguru , Van Diepen Sean , Lorusso Roberto , Krittanawong Chayakrit
Published: August 2023

Cardiogenic shock is a critical condition of low cardiac output resulting in insufficient systemic perfusion and end-organ dysfunction. Though significant advances have been achieved in reperfusion therapy and mechanical circulatory support, cardiogenic shock continues to be a life-threatening condition associated with a high rate of complications and excessively high patient mortality, reported to be between 35% and 50%. Extracorporeal membrane oxygenation can provide full cardiopulmonary support, has been increasingly used in the last two decades, and can be used to restore systemic end-organ hypoperfusion. However, a paucity of randomized controlled trials in combination with high complication and mortality rates suggest the need for more research to better define its efficacy, safety, and optimal patient selection. In this review, we provide an updated review on VA-ECMO, with an emphasis on its application in cardiogenic shock, including indications and contraindications, expected hemodynamic and echocardiographic findings, recommendations for weaning, complications, and outcomes. Furthermore, specific emphasis will be devoted to the two published randomized controlled trials recently presented in this setting.
Mismatch between Clinical–Functional and Radiological Outcome in Tibial Plateau Fractures: A Retrospective Study
Authors: Bormann Markus , Bitschi David , Neidlein Claas , Berthold Daniel P. , Jörgens Maximilian , Pätzold Robert , Watrinet Julius , Böcker Wolfgang , Holzapfel Boris Michael , Fürmetz Julian
Published: August 2023

Background: The evaluation of tibial plateau fractures (TPF) encompasses the assessment of clinical–functional and radiological parameters. In this study, the authors aimed to investigate the potential correlation between these parameters by utilizing both the clinical–functional and the modified radiological Rasmussen score. Methods: In this retrospective monocentric study conducted at a level-I trauma center, patients who underwent surgery between January 2014 and December 2019 due to a TPF were included. The clinical–functional Rasmussen score prior to the injury, at 1-year postoperatively, and during the last follow-up (minimum 18 months) was assessed using a standardized questionnaire. Additionally, the modified radiological Rasmussen score was determined at the 1-year postoperative mark using conventional radiographs in two planes. Results: A total of 50 patients were included in this study, comprising 40% (n = 20) men, and 60% (n = 30) women, with an average age of 47 ± 11.8 years (range 26–73 years old). Among them, 52% (n = 26) had simple fractures (classified according to Schatzker I–III), while 48% (n = 24; according to Schatzker IV–VI) had complex fractures. The mean follow-up was 3.9 ± 1.6 years (range 1.6–7.5 years). The functional Rasmussen score assessed before the injury and at follow-up showed an “excellent” average result. However, there was a significant difference in the values of complex fractures compared to before the injury. One year postoperatively, both the clinical–functional score and the modified radiological score demonstrated a “good” average result. The “excellent” category was more frequently observed in the functional score, while the “fair” category was more common in the radiological score. There was no agreement between the categories in both scores in 66% of the cases. Conclusions: The data from this retrospective study demonstrated that patients with TPF are able to achieve a nearly equivalent functional level in the medium-term after a prolonged recovery period, comparable to their pre-injury state. However, it is important to note that the correlation between clinical–functional and radiological parameters is limited. Consequently, in order to create prospective outcome scores, it becomes crucial to objectively assess the multifaceted nature of TPF injuries in more detail, both clinically and radiologically.
Clinical Characteristics, Neuroimaging Markers, and Outcomes in Patients with Cerebral Amyloid Angiopathy: A Prospective Cohort Study
Authors: Theodorou Aikaterini , Palaiodimou Lina , Papagiannopoulou Georgia , Kargiotis Odysseas , Psychogios Klearchos , Safouris Apostolos , Bakola Eleni , Chondrogianni Maria , Kotsali-Peteinelli Vasiliki , Melanis Konstantinos , Tsibonakis Athanasios , Andreadou Elissavet , Vasilopoulou Sofia , Lachanis Stefanos , Velonakis Georgios , Tzavellas Elias , Tzartos John S. , Voumvourakis Konstantinos , Paraskevas Georgios P. , Tsivgoulis Georgios
Published: August 2023

Background and purpose: Sporadic cerebral amyloid angiopathy (CAA) is a small vessel disease, resulting from progressive amyloid-β deposition in the media/adventitia of cortical and leptomeningeal arterioles. We sought to assess the prevalence of baseline characteristics, clinical and radiological findings, as well as outcomes among patients with CAA, in the largest study to date conducted in Greece. Methods: Sixty-eight patients fulfilling the Boston Criteria v1.5 for probable/possible CAA were enrolled and followed for at least twelve months. Magnetic Resonance Imaging was used to assess specific neuroimaging markers. Data regarding cerebrospinal fluid biomarker profile and Apolipoprotein-E genotype were collected. Multiple logistic regression analyses were performed to identify predictors of clinical phenotypes. Cox-proportional hazard regression models were used to calculate associations with the risk of recurrent intracerebral hemorrhage (ICH). Results: Focal neurological deficits (75%), cognitive decline (57%), and transient focal neurological episodes (TFNEs; 21%) were the most common clinical manifestations. Hemorrhagic lesions, including lobar cerebral microbleeds (CMBs; 93%), cortical superficial siderosis (cSS; 48%), and lobar ICH (43%) were the most prevalent neuroimaging findings. cSS was independently associated with the likelihood of TFNEs at presentation (OR: 4.504, 95%CI:1.258–19.088), while multiple (>10) lobar CMBs were independently associated with cognitive decline at presentation (OR:5.418, 95%CI:1.316–28.497). cSS emerged as the only risk factor of recurrent ICH (HR:4.238, 95%CI:1.509–11.900) during a median follow-up of 20 months. Conclusions: cSS was independently associated with TFNEs at presentation and ICH recurrence at follow-up, while a higher burden of lobar CMBs with cognitive decline at baseline. These findings highlight the prognostic value of neuroimaging markers, which may influence clinical decision-making.
Complicated Clinical Course of a Patient with Multivisceral Cystic Echinococcosis Requiring Extensive Surgical and Medical Treatment
Authors: Popa Gabriela Loredana , Popa Alexandru Cosmin , Mastalier Bogdan , Crețu Carmen Michaela , Popa Mircea Ioan
Published: August 2023

Cystic echinococcosis is an often-overlooked condition that otherwise negatively impacts both the individual and the community, prompting major public health concerns. Early diagnosis and treatment, as well as collaboration between specialties including surgery and parasitology, are essential for avoiding complications and disease relapse. To better illustrate this, we present the case of an elderly person with a rare localization of the disease at the muscular level. The patient underwent numerous surgical interventions, and received multiple courses of antiparasitic treatment over the course of 40 years as a result of the multivisceral dissemination of the parasite.
Nonalcoholic Fatty Liver Disease in Patients with Type 2 Diabetes: Screening, Diagnosis, and Treatment
Authors: Ciardullo Stefano , Vergani Michela , Perseghin Gianluca
Published: August 2023

Nonalcoholic fatty liver disease (NAFLD), recently renamed metabolic dysfunction-associated steatotic liver disease (MASLD) affects ~70% of patients with type 2 diabetes (T2D), with ~20% showing signs of advanced liver fibrosis. Patients with T2D are at an increased risk of developing cirrhosis, liver failure, and hepatocellular carcinoma and their liver-related mortality is doubled compared with non-diabetic individuals. Nonetheless, the condition is frequently overlooked and disease awareness is limited both among patients and among physicians. Given recent epidemiological evidence, clinical practice guidelines recommend screening for NAFLD/MASLD and advanced liver fibrosis in patients with T2D. While many drugs are currently being tested for the treatment of NAFLD/MASLD, none of them have yet received formal approval from regulatory agencies. However, several classes of antidiabetic drugs (namely pioglitazone, sodium-glucose transporter 2 inhibitors, glucagon-like peptide 1 receptor agonists, and multi-agonists) have shown favorable effects in terms of liver enzymes, liver fat content and, in some occasions, on histologic features such as inflammation and fibrosis. Therefore, diabetologists have the opportunity to actively treat NAFLD/MASLD, with a concrete possibility of changing the natural history of the disease. In the present narrative review, we summarize evidence and clinical recommendations for NAFLD/MAFLD screening in the setting of T2D, as well as on the effect of currently available glucose-lowering drugs on hepatic endpoints.
Reciprocal Change of Cervical Spine after Posterior Spinal Fusion for Lenke Type 1 and 2 Adolescent Idiopathic Scoliosis
Authors: Mori Kanji , Takahashi Jun , Oba Hiroki , Mimura Tetsuhiko , Imai Shinji
Published: August 2023

Reciprocal sagittal alignment changes after adolescent idiopathic scoliosis (AIS) posterior corrective surgery have been reported in the cervical spine, but the evidence is not yet sufficient. Furthermore, much remains unknown about the effects of cervical kyphosis on clinical outcomes in AIS. Forty-five consecutive patients (4 males and 41 females) with AIS and Lenke type 1 or 2 curves underwent a posterior spinal fusion, and a minimum of 24-month follow-up was collected from our prospective database. We investigated radiographic parameters and SRS-22r. Before surgery, cervical kyphosis (cervical lordosis < 0°) was present in 89% and cervical hyperkyphosis (cervical lordosis < −10°) in 60%. There were no significant differences in age, sex, or Lenke type between the hyperkyphosis and the non-hyperkyphosis groups. Although cervical lordosis increased significantly after surgery, cervical kyphosis was observed in 73% of patients 2 years after surgery. We found a significant correlation between Δthoracic kyphosis (TK) and Δcervical lordosis. Preoperative cervical kyphosis, ΔT1 slope, and ΔTK were independently associated factors for postoperative cervical hyperkyphosis. The cervical hyperkyphosis group had significantly lower SRS-22r domains. In AIS corrective surgery, restoring TK leading to a gain of T1 slope may lead to an improvement of cervical sagittal alignment. Remaining cervical hyperkyphosis after AIS surgery may affect clinical outcomes.
Marginal Ulcer and Dumping Syndrome in Patients after Duodenal Switch: A Multi-Centered Study
Authors: Salame Marita , Teixeira Andre F. , Lind Romulo , Ungson Gilberto , Ghanem Muhammad , Abi Mosleh Kamal , Jawad Muhammad A. , Abu Dayyeh Barham K. , Kendrick Michael L. , Ghanem Omar M.
Published: August 2023

Background: The current design of biliopancreatic diversion with duodenal switch (BPD/DS) and single anastomosis duodenal–ileal bypass with sleeve (SADI-S) emphasizes the importance of the pylorus’ preservation to reduce the incidence of marginal ulcer (MU) and dumping. However, no institutional studies have yet reported data on their prevalence. We aimed to assess the incidence of MU and dumping after duodenal switch (DS) and identify the associative factors. Methods: A multi-center review of patients who underwent BPD/DS or SADI-S between 2008 and 2022. Baseline demographics, symptoms, and management of both complications were collected. Fisher’s exact test was used for categorical variables and the independent t-test for continuous variables. Results: A total of 919 patients were included (74.6% female; age 42.5 years; BMI 54.6 kg/m2) with mean follow-up of 31.5 months. Eight patients (0.9%) developed MU and seven (0.8%) had dumping. Patients who developed MU were more likely to be using non-steroidal anti-inflammatory drugs (NSAID) (p = 0.006) and have a longer operation time (p = 0.047). Primary versus revisional surgery, and BDP/DS versus SADI-S were not associated with MU or dumping. Conclusions: The incidences of MU and dumping after DS were low. NSAID use and a longer operation time were associated with an increased risk of MU, whereas dumping was attributed to poor dietary habits.
Don’t Throw the ‘Bio’ out of the Bio-Psycho-Social Model: Editorial for Spine Rehabilitation in 2022 and Beyond
Authors: Harrison Deed E. , Oakley Paul A. , Moustafa Ibrahim M.
Published: August 2023

Spinal injuries, disorders and disabilities are among the leading causes for work loss, suffering, and health care expenditures throughout the industrialized world [...]
Prevalence and Prognostic Implications of PSA Flares during Radium-223 Treatment among Men with Metastatic Castration Resistant Prostate Cancer
Authors: Sidhu Amanjot , Khan Nabeeha , Phillips Cameron , Briones Juan , Kapoor Anil , Zalewski Pawel , Fleshner Neil E. , Chow Edward , Emmenegger Urban
Published: August 2023

Radium-223 (Ra233) prolongs the survival of men with symptomatic bone-predominant metastatic castration-resistant prostate cancer (mCRPC). However, prostate-specific antigen (PSA) response patterns are not closely associated with Ra223 therapy outcomes. Herein, we sought to analyze the impact of Ra223-induced PSA flares on patient outcome. Using a retrospective cohort study of Ra223 treatment in four Ontario/Canada cancer centres, we identified 134 patients grouped into sub-cohorts according to distinct PSA response patterns: (i) initial PSA flare followed by eventual PSA decline; (ii) PSA response (≥30% PSA decrease within 12 weeks of treatment); and (iii) PSA non-response. We analyzed patient characteristics and outcome measures, including overall survival (OS), using the Kaplan-Meier method and log-rank testing. PSA flares were observed in 27 (20.2%), PSA responses in 11 (8.2%), and PSA non-responses in 96 (71.6%) patients. Amongst PSA flare patients, 12 presented with post-flare PSA decreases below baseline and 15 with PSA decreases below the flare peak but above baseline. Although only six flare patients achieved ≥30% PSA decreases below baseline, the median OS of all flare patients (16.8 months, 95% CI 14.9–18.7) was comparable to that of PSA responders and non-responders (p = 0.349). In summary, around 20% of mCRPC patients experience Ra223-induced PSA flares, whose outcome is similar to that of men with or without PSA responses. Further studies are needed regarding suitable biochemical surrogate markers of response to Ra223.
Isolation and Detection of Exosomal Mir210 Using Carbon Nanomaterial-Coated Magnetic Beads
Authors: Chinnappan Raja , Ramadan Qasem , Zourob Mohammed
Published: August 2023

MicroRNAs (miRNAs) are short non-coding RNAs that are found in various cellular compartments and play an important role in regulating gene expression. Extracellular miRNAs, such as those found within extracellular vesicles such as exosomes are involved in cell-to-cell communication. The intercellular transfer of miRNAs has been implicated in various diseases’ pathogenesis including cancer and has been studied extensively as potential cancer biomarkers. However, the extraction of miRNA from exosomes is still a challenging task. The current nucleic acid extraction assays are expensive and labor-intensive. In this study, we demonstrated a microfluidic device for aptamer-based magnetic separation of the exosomes and subsequent detection of the miRNA using a fluorescence switching assay, which was enabled by carbon nanomaterials coated on magnetic beads. In the OFF state, the fluorophore-labelled cDNA is quenched using carbon nanomaterials. However, when the target miRNA210 is introduced, the cDNA detaches from the bead’s surface, which leads to an increase in the fluorescence intensity (ON state). This increment was found to be proportional to miRNA concentration within the dynamic range of 0–100 nM with a detection limit of 5 pM. The assay was validated with spiked miRNA using the standard RT-PCR method. No notable cross-reactivity with other closely related miRNAs was observed. The developed method can be utilized for the minimally invasive detection of cancer biomarkers.
PP-JPEG: A Privacy-Preserving JPEG Image-Tampering Localization
Authors: Jena Riyanka , Singh Priyanka , Mohanty Manoranjan
Published: August 2023

The widespread availability of digital image-processing software has given rise to various forms of image manipulation and forgery, which can pose a significant challenge in different fields, such as law enforcement, journalism, etc. It can also lead to privacy concerns. We are proposing that a privacy-preserving framework to encrypt images before processing them is vital to maintain the privacy and confidentiality of sensitive images, especially those used for the purpose of investigation. To address these challenges, we propose a novel solution that detects image forgeries while preserving the privacy of the images. Our method proposes a privacy-preserving framework that encrypts the images before processing them, making it difficult for unauthorized individuals to access them. The proposed method utilizes a compression quality analysis in the encrypted domain to detect the presence of forgeries in images by determining if the forged portion (dummy image) has a compression quality different from that of the original image (featured image) in the encrypted domain. This approach effectively localizes the tampered portions of the image, even for small pixel blocks of size 10×10 in the encrypted domain. Furthermore, the method identifies the featured image’s JPEG quality using the first minima in the energy graph.
Resolved CFD-DEM Simulation of Free Settling of Polyhedral Particles with Various Orientations: Insights Provided by Oscillation Behavior in Quiescent Liquid
Authors: Zhang Zhimin , Qiu Yuanyi , Xiong Hao , Tang Rui
Published: August 2023

Particle settling is the process by which particulates move toward the bottom of a liquid, which can affect the sediment transport and energy balance of marine systems. However, the deficiency in understanding the resolved fluid–particle interactions with complex boundaries in the settling process awaits resolution. This study employs a hybrid approach that combines computational fluid dynamics (CFD) with the discrete element method (DEM) to fully simulate the free-settling behavior of polyhedral particles in water. The accuracy of the method is verified by comparing numerical results with experimental data of ellipsoidal particle settling. Two series of tests with horizontal and vertical particle release directions are established to investigate the effects of particle shape features, such as the aspect ratio (AR) and corner (C), on the particles’ mechanical behavior and hydrodynamic characteristics. The results demonstrate that particle shape exerts a substantial influence on fluid resistance, rotational motion, and fluid disturbance throughout the settling process. The maximum velocities in vertically released cases are roughly 1.2–1.3 times greater than those in horizontally released cases. The study highlights the potency of the resolved CFD-DEM method as a robust technique for comprehending fluid–particle phenomena within the marine geotechnical engineering, including sedimentation and erosion of seabed sediments.
Weakening of the Geostrophic Component of the Gulf Stream: A Positive Feedback Loop on the Melting of the Arctic Ice Sheet
Authors: Pinault Jean-Louis
Published: August 2023

The North Atlantic gyre experiences both a significant temperature rise at high latitudes and a considerable weakening of the geostrophic component of the Gulf Stream, which is reflected by the 64-year fundamental gyral Rossby wave (GRW). This singular behavior compared to the South Atlantic and South Indian Ocean gyres highlights a feedback loop of Arctic ice sheet melting on mid-latitude Atlantic Ocean temperature. The warming of the northern oceanic gyre at high latitudes due to the retreat of Arctic ice sheet via the Labrador Current decreases the thermal gradient between the high and low latitudes of the north Atlantic gyre. This results in a weakening of the geostrophic forces at the basin scale and a reduction in the amplitude of the GRWs. Reducing the amplitude of the variation of the upward and downward movement of the pycnocline modifies air–sea interactions, weakening vertical mixing as well as the evaporation processes and the departure of latent heat when the pycnocline rises. The resulting thermal anomaly stretching along the Gulf Stream from where it leaves the American continent is partly transferred to the Arctic sea ice via the drift current and thermohaline circulation, which contributes to the retreat of the ice sheet, and the closing of the feedback loop. The 64-year-period GRW should disappear around 2050 if its damping continues linearly, favoring an increasingly rapid warming of the ocean at mid-latitudes. These interactions are less acute in the southern hemisphere due to the circumpolar current.
Study on the Effect of Nodal Configuration on the Mechanical Properties of Hexa-Ligamentous Chiral Honeycombs
Authors: Wang Zhiping , Chen Gang , Cao Xiaofei , Chen Wei , Li Chun Bao , Li Xiaobin
Published: August 2023

To investigate the effect of nodal configuration on the mechanical properties of hexachiral honeycombs, three specimens, namely, a standard honeycomb, a thickened-node honeycomb, and a filled-node honeycomb, were prepared using 3D-printing technology. Several quasi-static compression tests were performed, which revealed that nodal reinforcement can inhibit nodal aberrations during ligament winding, thus facilitating the “rotational” mechanism and improving the negative Poisson’s ratio properties of the honeycomb. Experiments performed using the finite element method showed that nodal reinforcement mainly played a role in the stage of stress rise, and the role of nodal filling was more significant than that of nodal thickening. Low-strain standard honeycombs presented the highest specific absorption energy. However, the specific absorption energy of the filled-node honeycomb and the thickened-node honeycomb exceeded that of the standard honeycomb at a strain of 0.71. The conclusions presented herein can aid in the optimal design of honeycombs and contribute to the design of protective structures.
The Invasive Seaweed Agarophyton vermiculophyllum from Oualidia Lagoon (Northwestern Moroccan Atlantic Coast) as a Source of Agar: Yield, Chemical Characteristics, and Rheological Properties
Authors: Nadri Amal , Belattmania Zahira , Chaouti Abdellatif , Bentiss Fouad , Jama Charafeddine , Hmimid Fouzia , Reani Abdeltif , Sabour Brahim
Published: August 2023

Agar is a hydrophilic biopolymer extracted from red seaweed. This phycocolloid consists of two components: agarose and agaropectin. In the present work, agar extracted from the invasive red seaweed Agarophyton vermiculophyllum was characterized using physical, chemical, and spectroscopic analyses to investigate the effect of alkaline pretreatment on agar properties. Two extraction conditions, native and alkali-pretreated agars, were comparatively studied. The native yield (28.4 ± 0.9%) was higher than that of the alkaline-pretreated agar (20.4 ± 0.8%). The alkali-pretreated agar showed higher gel strength (763.8 ± 57.0g cm−2), gelling (36.5 ± 0.9 °C), and melting (83.2 ± 0.6 °C) temperatures and increased 3,6-anhydrogalactose (26.2 ± 1.9%) and decreased sulfate contents (6.2 ± 0.8%) compared with native agar (gel strength: 204.8 ± 17.10g cm−2, gelling temperature: 29.5 ± 0.9 °C, melting temperature: 73.4 ± 0.7 °C, 3,6-AG content: 13.8 ± 0.7%, sulfate content: 10.5 ± 0.5%). The alkaline pretreatment improved the agar’s gelling properties and significantly influenced its chemical properties. In view of the obtained results, A. vermiculophyllum might potentially be thought of as a viable source for the agar industry in Morocco, serving as a local source of agar.
Isolation and Identification of Non-Saccharomyces Yeast Producing 2-Phenylethanol and Study of the Ehrlich Pathway and Shikimate Pathway
Authors: Zhou Rong , Song Qingyi , Xia Huili , Song Na , Yang Qiao , Zhang Xiaoling , Yao Lan , Yang Shihui , Dai Jun , Chen Xiong
Published: August 2023

2-phenylethanol (2-PE) has been widely utilized as an aromatic additive in various industries, including cosmetics, beer, olive oil, tea, and coffee, due to its rose-honey-like aroma. However, no reports have investigated the production of 2-PE by Starmerella bacillaris. Here, S. bacillaris (syn., Candida zemplinina, and named strain R5) was identified by analysis of morphology, physiology and biochemistry, and 26S rRNA and ITS gene sequence. Then, based on the analysis of whole-genome sequencing and comparison with the KEGG database, it was inferred that strain R5 could synthesize 2-PE from L-phe or glucose through the Ehrlich pathway or shikimate pathway. For further verification of the 2-PE synthesis pathway, strain R5 was cultured in M3 (NH₄⁺), M3 (NH₄⁺ + Phe), and M3 (Phe) medium. In M3 (Phe) medium, the maximum concentration of 2-PE reached 1.28 g/L, which was 16-fold and 2.29-fold higher than that in M3 (NH₄⁺) and M3 (Phe + NH₄⁺) media, respectively. These results indicated that 2-PE could be synthesized by strain R5 through the shikimate pathway or Ehrlich pathway, and the biotransformation from L-phe to 2-PE was more efficient than that from glucose. The qRT-PCR results suggested that compared to M3 (Phe + NH₄⁺) medium, the mRNA expression levels of YAT were 124-fold and 86-fold higher in M3 (Phe) and M3 (NH₄⁺) media, respectively, indicating that the transport of L-phe was inhibited when both NH₄⁺ and Phe were present in the medium. In the M3 (Phe) and M3 (Phe + NH₄⁺) media, the mRNA expression level of ADH5 was higher than PDC, hisC, GOT1, and YAT, and it was 2.6 times higher and 2.48 times higher, respectively, compared to the M3 (NH₄⁺) medium, revealing that the key gene catalyzing the dehydrogenation of benzaldehyde to 2-PE is ADH5. Furthermore, strain R5 exhibits tolerance to high concentrations of 2-PE, reaching 3 g/L, which conferred an ideal tolerance to 2-PE. In summary, the synthesis pathway of 2-PE, mainly for the Ehrlich pathway, was proved for the first time in S. bacillaris, which had not been previously explored and provided a basis for non-Saccharomyces yeast-producing 2-PE and its applications.
ReLiFiRa (Real Life Filgotinib in Rheumatoid Arthritis): Retrospective Study of Efficacy and Safety in Common Clinical Practice
Authors: Benucci Maurizio , Bardelli Marco , Cazzato Massimiliano , Laurino Elenia , Bartoli Francesca , Damiani Arianna , Li Gobbi Francesca , Panaccione Anna , Di Cato Luca , Niccoli Laura , Frediani Bruno , Mosca Marta , Guiducci Serena , Cantini Fabrizio
Published: August 2023

Background: Filgotinib (FIL) is a selective JAK1 inhibitor with an affinity 30-fold higher than JAK2, approved to treat moderate to severe active rheumatoid arthritis (RA), in adults with inadequate response or intolerance to one or more disease-modifying antirheumatic drugs (DMARDs). Methods: We conducted a retrospective, multicentric study in order to evaluate efficacy and safety of FIL 200 mg daily therapy, after 3 and 6 months, in 120 patients affected by RA, managed in Tuscany and Umbria rheumatological centers. The following clinical records were analyzed: demographical data, smoking status, previous presence of comorbidities (Herpes zoster -HZ- infection, venous thromboembolism -VTE-, major adverse cardiovascular events -MACE-, cancer, diabetes, and hypertension), disease duration, presence of anti-citrullinated protein antibodies (ACPA), rheumatoid factor (RF), number of biological failures, and prior csDMARDs utilized. At baseline, and after 3 (T3) and 6 (T6) months of FIL therapy, we evaluated mean steroid dosage, csDMARDs intake, clinimetric indexes (DAS28, CDAI, HAQ, patient and doctor PGA, VAS), erythrocyte sedimentation rate (ESR), C-reactive protein (CRP), and body mass index (BMI). Results: At baseline, the mean disease duration was 9.4 ± 7.5 years; the prevalence of previous HZ infection, VTE, MACE, and cancer was respectively 4.12%, 0%, 7.21%, and 0.83%, respectively. In total, 76.3% of patients failed one or more biologics (one biological failure, 20.6%; two biological failures, 27.8%; three biological failures, 16.5%; four biological failures, 10.3%; five biological failures, 1.1%). After 3 months of FIL therapy, all clinimetric index results significantly improved from baseline, as well as after 6 months. Also, ESR and CRP significatively decreased at T3 and T6. Two cases of HZ were recorded, while no new MACE, VTE, or cancer were recorded during the observation time. Conclusion: Despite the limitations of the retrospective study and of the observational period of only 6 months, real-life data on the treatment of RA patients with FIL demonstrate that this Jak inhibitor therapy is safe in terms of CV, VTE events, and occurrence of cancer, and is also effective in a population identified as “difficult to treat” due to failure of previous b-DMARD therapy.
Genetical Signature—An Example of a Personalized Skin Aging Investigation with Possible Implementation in Clinical Practice
Authors: Sepetiene Ramune , Patamsyte Vaiva , Valiukevicius Paulius , Gecyte Emilija , Skipskis Vilius , Gecys Dovydas , Stanioniene Zita , Barakauskas Svajunas
Published: August 2023

We conducted a research study to create the groundwork for personalized solutions within a skin aging segment. This test utilizes genetic and general laboratory data to predict individual susceptibility to weak skin characteristics, leveraging the research on genetic polymorphisms related to skin functional properties. A cross-sectional study was conducted in a collaboration between the Private Clinic Medicina Practica Laboratory (Vilnius, Lithuania) and the Public Institution Lithuanian University of Health Sciences (Kaunas, Lithuania). A total of 370 participants agreed to participate in the project. The median age of the respondents was 40, with a range of 19 to 74 years. After the literature search, we selected 15 polymorphisms of the genes related to skin aging, which were subsequently categorized in terms of different skin functions: SOD2 (rs4880), GPX1 (rs1050450), NQO1 (rs1800566), CAT (rs1001179), TYR (rs1126809), SLC45A2 (rs26722), SLC45A2 (rs16891982), MMP1 (rs1799750), ELN (rs7787362), COL1A1 (rs1800012), AHR (rs2066853), IL6 (rs1800795), IL1Beta (rs1143634), TNF-α (rs1800629), and AQP3 (rs17553719). RT genotyping, blood count, and immunochemistry results were analyzed using statistical methods. The obtained results show significant associations between genotyping models and routine blood screens. These findings demonstrate the personalized medicine approach for the aging segment and further add to the growing literature. Further investigation is warranted to fully understand the complex interplay between genetic factors, environmental influences, and skin aging.
Association of the APOE Gene Polymorphism with Depression in White Adults in the WHO “MONICA-Psychosocial” Program
Authors: Gafarov Valery , Gromova Elena , Shakhtshneider Elena , Gagulin Igor , Gafarova Almira
Published: August 2023

The APOE gene polymorphism is associated with the risk of the development of several neurological disorders. The aim of the study was to investigate the association of the APOE gene polymorphism with depression in the white adult population aged 25–64 years in Novosibirsk (Western Siberia). The third screening of the WHO program “MONICA-psychosocial” was conducted in 1994–1995. In total, 403 men (the average age was 34 ± 0.4 years, the response was 71%) and 531 women (the average age was 35 ± 0.4 years, the response was 72%) of the open population of residents aged 25–64 years of the Oktyabrsky district of Novosibirsk were examined. The “MONICA-MOPSY” psychosocial questionnaire was used to assess depression. A high level of depression was found in 12.8% of the population: in 8.9% of men and in 15.8% of women. The frequencies of APOE gene polymorphism genotypes ε2/3, ε2/4, ε3/3, ε3/4, and ε4/4 were 14.9%, 3.1%, 61.6%, 17.5%, and 2.9%, respectively. Carrying the ε3/4 genotype of the APOE gene increased the odds of developing major depression by 2.167 times (95% CI 1.100–4.266) compared to carrying the ε3/3 genotype of the APOE gene in people without depression (χ2 = 5.120 df = 1 p = 0.024). Carriers of the ε4 allele were 2.089 times (95% CI 1.160–3.761) more likely to have a high level of depression than those without this allele and no depression (χ2 = 6.148 df = 1 p = 0.013), and 2.049 times (95% CI 1.117–3.758) more likely to have a moderate level of depression than those without this allele (χ2 = 5.470 df = 1 p < 0.019). The ε4 allele of the APOE gene is associated with a high level of depression.