bluuebunny/crossref_metadata_2025 huggingface dataset:
Can Behavioral “Nudges” Improve Compliance? The Case of Colombia Social Protection Contributions
Authors: Alm James , Cifuentes Laura Rosales , Niño Carlos Mauricio Ortiz , Rocha Diana
Published: October 2019

The Government of Colombia imposes a variety of taxes that must be paid by individual wage earners, called in their entirety “social protection contributions”. Since 2007 individual payments have been collected using an on-line mechanism. In order to improve compliance, the Government used a controlled field experiment in which various “pop-up messages” were sent to individuals when making their on-line payments, as behavioral “nudges”. We examine the impact of these nudges on individual reporting behavior. We find mixed evidence that these messages increased compliance rates relative to a control group that received a so-called “neutral” message. However, we also demonstrate that the use as the control group of individuals receiving a so-called “neutral” message creates considerable bias; that is, the receipt of any message of any type clearly influences behavior. Instead, we show that the appropriate control group should be individuals who receive no message at all. When this control group is used, we find that self-employed individuals generally increase their contributions; individuals who are making declarations on behalf of all employees in their company are less likely to respond to messages in a systematic way.
‘Humorous Is the Only Truthful Way to Tell a Sad Story’: Jonathan Safran Foer and Third Generation Holocaust Representation
Authors: Coakley Sarah
Published: October 2019

Jonathan Safran Foer’s representation of the Holocaust in his first novel, Everything is Illuminated, has been the subject of much controversy and critical debate. Several critics and Holocaust survivors have objected to the work for the lack of historical accuracy in its mythological narrative and the irreverence of its humour. However, such responses fail to take into account its specific form of generational representation: The Holocaust of Everything is Illuminated is always perceived through a third-generation lens, and its provocative elements instead highlight aspects of the experiences of the grandchildren of survivors. With this in mind, this paper examines Foer’s approach to the Holocaust in Everything is Illuminated and Liev Schreiber’s film adaptation (2005), making specific reference to the challenges faced by the third generation. Drawing upon theories of the transgenerational transmission of trauma and postmemory, it will explore the roles of creativity and humour in resilience, in addition to the reconstruction of a historical narrative under threat of erasure. Ultimately, by offsetting the tendencies to reduce the complexity of the Holocaust into unequivocal moralities (as exhibited in the film adaptation) with the idiosyncrasies of the third-generation experience, an alternative contextual perspective on the Holocaust is propounded, containing its own discrete set of ethical questions and concerns.
Conflict in Catalonia: A Sociological Approximation
Authors: Miley Thomas , Garvía Roberto
Published: October 2019

This article follows the approach originally pioneered by Juan Linz to the empirical study of nationalism. We make use of original survey data to situate the emergent social division around the question of independence within a broader constellation of power relations. We bring into focus a variety of demographic, cultural, behavioral and attitudinal indicators with which this division is associated. We emphasize the special salience of language practices and ideologies in conditioning, if not determining, attitudes towards independence. We stress the continuing legacy of what Linz famously referred to as a “three-cornered conflict” among “regional nationalists, the central government and immigrant workers,” which has long conditioned democratic politics in the region. More concretely, we show how the reinforcing cleavages of language and class are reflected in, and indeed have been exacerbated by, the ongoing political conflict between pro-independence and pro-unionist camps in Catalonia. At the same time, we highlight that near half of the Catalan citizenry has come to register a rather intense preference in favor of independence, and we conclude that this sociological reality renders it quite difficult for Spanish authorities to enforce the will of the Spanish majority without appearing to tyrannize the Catalan minority.
Comparative Chromosome Mapping of Musk Ox and the X Chromosome among Some Bovidae Species
Authors: Proskuryakova Anastasia A. , Kulemzina Anastasia I. , Perelman Polina L. , Yudkin Dmitry V. , Lemskaya Natalya A. , Okhlopkov Innokentii M. , Kirillin Egor V. , Farré Marta , Larkin Denis M. , Roelke-Parker Melody E. , O’Brien Stephen J. , Bush Mitchell , Graphodatsky Alexander S.
Published: October 2019

Bovidae, the largest family in Pecora infraorder, are characterized by a striking variability in diploid number of chromosomes between species and among individuals within a species. The bovid X chromosome is also remarkably variable, with several morphological types in the family. Here we built a detailed chromosome map of musk ox (Ovibos moschatus), a relic species originating from Pleistocene megafauna, with dromedary and human probes using chromosome painting. We trace chromosomal rearrangements during Bovidae evolution by comparing species already studied by chromosome painting. The musk ox karyotype differs from the ancestral pecoran karyotype by six fusions, one fission, and three inversions. We discuss changes in pecoran ancestral karyotype in the light of new painting data. Variations in the X chromosome structure of four bovid species nilgai bull (Boselaphus tragocamelus), saola (Pseudoryx nghetinhensis), gaur (Bos gaurus), and Kirk’s Dikdik (Madoqua kirkii) were further analyzed using 26 cattle BAC-clones. We found the duplication on the X in saola. We show main rearrangements leading to the formation of four types of bovid X: Bovinae type with derived cattle subtype formed by centromere reposition and Antilopinae type with Caprini subtype formed by inversion in XSB1.
HiCNN2: Enhancing the Resolution of Hi-C Data Using an Ensemble of Convolutional Neural Networks
Authors: Liu Tong , Wang Zheng
Published: October 2019

We present a deep-learning package named HiCNN2 to learn the mapping between low-resolution and high-resolution Hi-C (a technique for capturing genome-wide chromatin interactions) data, which can enhance the resolution of Hi-C interaction matrices. The HiCNN2 package includes three methods each with a different deep learning architecture: HiCNN2-1 is based on one single convolutional neural network (ConvNet); HiCNN2-2 consists of an ensemble of two different ConvNets; and HiCNN2-3 is an ensemble of three different ConvNets. Our evaluation results indicate that HiCNN2-enhanced high-resolution Hi-C data achieve smaller mean squared error and higher Pearson’s correlation coefficients with experimental high-resolution Hi-C data compared with existing methods HiCPlus and HiCNN. Moreover, all of the three HiCNN2 methods can recover more significant interactions detected by Fit-Hi-C compared to HiCPlus and HiCNN. Based on our evaluation results, we would recommend using HiCNN2-1 and HiCNN2-3 if recovering more significant interactions from Hi-C data is of interest, and HiCNN2-2 and HiCNN if the goal is to achieve higher reproducibility scores between the enhanced Hi-C matrix and the real high-resolution Hi-C matrix.
Molecular Cloning and Characterization of SYCP3 and TSEG2 Genes in the Testicles of Sexually Mature and Immature Yak
Authors: Kalwar Qudratullah , Chu Min , Ahmad Anum Ali , Ma Xiaoming , Zhang Renzheng , Ma Fulong , Xie Jianpeng , Ding Xuezhi , Wu Xiaoyun , Bao Pengjia , Yan Ping
Published: October 2019

Testis-specific genes play an essential part in the centromere union during meiosis in male germ cells, spermatogenesis, and in fertility. Previously, there was no research report available on the expression pattern of SYCP3 and TSEG2 genes in different ages of yaks. Therefore, the current research compared the expression profiling of SYCP3 and TSEG2 genes in testes of yaks. The expression pattern of SYCP3 and TSEG2 mRNA was investigated using qPCR, semi-quantitative PCR, western blot, immunohistochemistry, and molecular bioinformatics. Our findings displayed that SYCP3 and TSEG2 genes were prominently expressed in the testicles of yaks as compared to other organs. On the other hand, the protein encoded by yak SYCP3 contains Cor1/Xlr/Xmr conserved regions, while the protein encoded by yak TSEG2 contains synaptonemal complex central element protein 3. Additionally, multiple alignments sequences indicated that proteins encoded by Datong yak SYCP3 and TSEG2 were highly conserved among mammals. Moreover, western blot analysis specified that the molecular mass of SYCP3 protein was 34-kDa and TSEG2 protein 90-kDa in the yak. Furthermore, the results of immunohistochemistry also revealed the prominent expression of these proteins in the testis of mature yaks, which indicated that SYCP3 and TSEG2 might be essential for spermatogenesis, induction of central element assembly, and homologous recombination.
Multi-Tissue Transcriptomes Yield Information on High-Altitude Adaptation and Sex-Determination in Scutiger cf. sikimmensis
Authors: Hofmann Sylvia , Kuhl Heiner , Baniya Chitra Bahadur , Stöck Matthias
Published: October 2019

The Himalayas are one of earth’s hotspots of biodiversity. Among its many cryptic and undiscovered organisms, including vertebrates, this complex high-mountain ecosystem is expected to harbour many species with adaptations to life in high altitudes. However, modern evolutionary genomic studies in Himalayan vertebrates are still at the beginning. Moreover, in organisms, like most amphibians with relatively high DNA content, whole genome sequencing remains bioinformatically challenging and no complete nuclear genomes are available for Himalayan amphibians. Here, we present the first well-annotated multi-tissue transcriptome of a Greater Himalayan species, the lazy toad Scutiger cf. sikimmensis (Anura: Megophryidae). Applying Illumina NextSeq 500 RNAseq to six tissues, we obtained 41.32 Gb of sequences, assembled to ~111,000 unigenes, translating into 54362 known genes as annotated in seven functional databases. We tested 19 genes, known to play roles in anuran and reptile adaptation to high elevations, and potentially detected diversifying selection for two (TGS1, SENP5) in Scutiger. Of a list of 37 genes, we also identify 27 candidate genes for sex determination or sexual development, all of which providing the first such data for this non-model megophryid species. These transcriptomes will serve as a valuable resource for further studies on amphibian evolution in the Greater Himalaya as a biodiversity hotspot.
A New Methodology for Use by a Single Caregiver to Bathe Bedridden Elderly Persons Using Advanced Mechatronic Systems
Authors: Bezerra Karolina , Machado José , Carvalho Vítor , Soares Filomena , Matos Demétrio , Castro Marcelo , Pereira Filipe , Lopes Hugo
Published: October 2019

In the framework of this paper, we aimed to propose a methodology for giving baths to elderly, bedridden persons, when this task is performed by a single caregiver. Usually, two caregivers are required for nursing a bedridden patient, especially when certain important tasks are needed (e.g., bathing the patient), but this is not always possible. The entire study considers the primary user’s perspective—the caregiver—who is responsible for a wide range of tasks; thus, suffering physical and psychological exhaustion over time. A physical prototype has been developed for allowing caregivers to perform tests in a life-like environment, by means of the device and the methodology. This technology, therefore, will represent an important contribution to the quality of life of caregivers. Considering an increase in the share of the elderly population and the related problems that arise in daily care, this project intends to be beneficial contemporarily. The presented methodology has been successfully tested and validated.
The Storage and Water Quality Characteristics of Rungiri Quarry Reservoir in Kiambu, Kenya, as a Potential Source of Urban Water
Authors: Kilonzo Winfred , Home Patrick , Sang Joseph , Kakoi Beatrice
Published: October 2019

Urbanization has caused limitations on water resources, while climate change has reduced amounts of surface water in some parts of the world. Kikuyu, a suburban area in Kiambu county, Kenya, is facing this challenge. The major challenge in the study is scarcity of potable water, resulting in inadequate water supply to Kikuyu residents. Currently, only 63.6% of the population is being supplied with water by Kikuyu Water Company, the company mandated to supply water to the area. Water demand was 2972 m3/day in 2015 and was projected to be 3834 m3/day by 2025. This has put pressure on the already exploited clean water resources, making it necessary to seek additional sources of domestic water. Storage capacity and water quality of surface water bodies, especially small reservoirs whose water can be used to ease the demand, need to be assessed for supplemental water supply. This study aimed at assessing the suitability of the abandoned quarry reservoir as a source of potable urban water by determining its storage capacity characteristics and water quality status. Volume characteristics were determined using bathymetry survey in January 2019. Water samples were collected in January and August 2019 and analyzed for chemical, physical, and bacteriological quality, as per the American Public Health Association (APHA) standard methods for water and wastewater. Parameters were evaluated based on World Health Organization (WHO) and Kenya Bureau of Standards (KEBS) guidelines for drinking water, and rated based on the drinking water quality index (WQI). The reservoir’s maximum storage capacity was found to be 128,385 m3, the surface area was 17,699 m2, and the maximum depth was 15.11 m. Nineteen of the twenty-five investigated parameters were within the acceptable standards. However, the concentrations of manganese (Mn), cadmium (Cd), iron (Fe), turbidity, total coliforms, and Escherichia coli (E. coli) were above the acceptable limits. Manganese and iron levels increased with depth. The overall WQI of the reservoir was 82.51 and 85.85 in January and August, respectively. Therefore, based on WQI rating, the water scored a good quality rating and could be used for domestic supply upon treatment. The original achievement of this study is establishment of the volume of the water in the quarry as an additional source of water to the nearby community, along with water quality status.
High-Intensity Interval Training Versus Moderate-Intensity Continuous Training in Middle-Aged and Older Patients with Type 2 Diabetes: A Randomized Controlled Crossover Trial of the Acute Effects of Treadmill Walking on Glycemic Control
Authors: Mendes Romeu , Sousa Nelson , Themudo-Barata José Luís , Reis Victor Machado
Published: October 2019

Background: This study aimed to compare the acute effects of high-intensity interval training (HIIT) versus moderate-intensity continuous training (MICT) on glycemic control in middle-aged and older patients with type 2 diabetes (T2D), using treadmill walking as aerobic exercise mode. Methods: Fifteen patients with T2D (60.25 ± 3.14 years; glycated hemoglobin 7.03 ± 0.33%; medicated with metformin and/or gliptins), participated in a randomized controlled crossover trial. They underwent three experimental conditions (treadmill walking HIIT session (5 × (3 min at 70% of heart rate reserve (HRR) + 3 min at 30% HRR)); treadmill walking MICT session (30 min at 50% HRR); and a control session of rest (CON)) in random order and in the postprandial state. Measurements of capillary blood glucose (BG) were taken immediately before, during, and until 50 min after the experimental conditions. Results: Both HIIT and MICT treadmill walking sessions reduced BG levels during exercise and laboratory 50 min recovery period compared to CON (time*condition interaction effect; p < 0.001). The effect of HIIT was greater compared with MICT (p = 0.017). Conclusions: Treadmill walking HIIT seems a safe and more effective exercise strategy on immediate acute glycemic control compared with MICT in middle-aged and older patients with T2D under therapy with metformin and/or gliptins. Trial Registration Number: ISRCTN09240628.
Risk, Stigma, Trustworthiness, and Citizen Participation—A Multifaceted Analysis of Media Coverage of Dioxin Contamination in Midland, Michigan
Authors: Zhuang Jie , Cox Jeffrey G. , Chung Minwoong , Hamm Joseph A. , Zwickle Adam , Upham Brad L.
Published: October 2019

In the United States, more than 200 communities are designated by the U.S. Environmental Protection Agency as areas of concern for dioxins. Informing the public about potential risks associated with dioxins and delivering information about how to avoid such risks are essential activities. News coverage of environmental and health problems affects how members of the public assess those problems in terms of both severity and how they are understood, as well as the extent of attention given to the problem by policy-makers. To contextualize public and institutional responses to dioxin contamination and remediation in a dioxin-affected community, we assessed 176 newspaper articles published over 30 years concerning dioxin contamination in Midland, Michigan, in terms of risk, trust in institutions, environmental stigma, and citizen participation. Articles about dioxin contamination and remediation in Midland appeared in both domestic and international newspapers. Domestically, both national and local newspapers covered this issue. The risks for human health and the environment caused by exposure to dioxins were widely covered, with much less media attention given to the trustworthiness of the organizations responsible for managing the risk, environmental stigma, and citizen participation. News coverage of these four themes also changed significantly overtime. Overall, our findings highlight the important role of local news media in communicating risk information, guiding safe behaviors, and facilitating community-level decision-making.
Are Karst Rocky Desertification Areas Affected by Increasing Human Activity in Southern China? An Empirical Analysis from Nighttime Light Data
Authors: Shi Kaifang , Yang Qingyuan , Li Yuanqing
Published: October 2019

Due to remarkable socioeconomic development, an increasing number of karst rocky desertification areas have been severely affected by human activities in southern China. Effectively analyzing human activities in karst rocky desertification areas is a critical prerequisite for managing and restoring areas with tremendous negative impacts from desertification. At present, a timely and accurate way of quantifying the spatiotemporal variations of human activities in karst rocky desertification areas is still lacking. In this communication, we attempted to quantify human activities from the corrected Suomi National Polar-orbiting Partnership (NPP) Visible Infrared Imaging Radiometer Suite (VIIRS) Day/Night Band (DNB) nighttime light composite data from 2012 to 2018 based on statistical analysis. The results show that a significant increase of night lights could be clearly identified during the study period. The total nighttime lights (TL) related to severe karst rocky desertification (S) were particularly concentrated in Guizhou and Yunnan. The nighttime light intensity (LI) related to the S areas in Chongqing was the strongest due to its rapid socioeconomic development. The annual growth rate of nighttime lights (GL) has been slow or even negative in Guangdong because of its various karst rocky desertification restoration programs. This communication could provide an effective approach for quantifying human activities and provide useful information about where prompt attention is required for policy-making on the restoration of the karst rocky desertification areas.
Associations between the Home Physical Environment and Children’s Home-Based Physical Activity and Sitting
Authors: Sheldrick Michael P. , Maitland Clover , Mackintosh Kelly A. , Rosenberg Michael , Griffiths Lucy J. , Fry Richard , Stratton Gareth
Published: October 2019

It is important to understand the correlates of children’s physical activity (PA) and sitting at home, where children spend significant time. The home social environment has an important influence; however, much less is known about the home physical environment. Therefore, the study aimed to assess relationships between the physical environment and children’s sitting and PA at home. In total, 235 child-parent dyads were included in the analyses. Children spent 67% of their time at home sitting. Linear regression analyses examined associations between physical home environmental factors obtained via an audit and children’s (55% girl, 10.2 ± 0.7) objective PA and sitting at home. Following adjustment for socio-demographics and social environmental factors, an open plan living area (OPLA), musical instrument accessibility and availability, and perceived house size were negatively and positively associated, whereas media equipment accessibility and availability was positively and negatively associated with sitting and standing, respectively. Additionally, an OPLA was positively associated with total and moderate-to-vigorous PA. Furthermore, sitting breaks were positively associated with objective garden size and negatively associated with digital TV. The physical home environment may have an important influence on children’s sitting, standing and PA at home; therefore, interventions that target this environment are needed.
The Effects of 12 Weeks of a Combined Exercise Program on Physical Function and Hormonal Status in Elderly Korean Women
Authors: Im Ji Yu , Bang Hyun Seok , Seo Dae Yun
Published: October 2019

Aging causes a decline in physical function and hormonal balance. Exercise can improve these parameters. However, the beneficial effects of a combined exercise program (Korean dance and yoga) on physical function and hormonal status in elderly women remain unknown. This study aims to investigate the effects of a 12-week combined exercise program on balance, flexibility, muscle strength, and hormonal status in elderly Korean women. Twenty-five healthy elderly women were recruited and randomly divided into the control (CON) and exercise (EXE) groups. The EXE group underwent the combined exercise program (60 min/day and 3 times/week) for 12 weeks. The two groups did not differ in body weight, lean body mass, fat mass, body fat percentage, or body mass index at baseline or in the changes following the experimental conditions. A significant time × group interaction was detected for anterior and posterior dynamic balance, static balance, and growth hormone (GH). After the combined exercise program, anterior dynamic balance, posterior dynamic balance, static balance, flexibility, muscle strength, GH, dehydroepiandrosterone-sulfate, and estrogen significantly increased in the EXE group compared to the CON group. In conclusion, the combined exercise program contributed to improvements in overall health, including physical function and hormonal status, in elderly Korean women.
Authentic Leadership and Employees’ Innovative Behaviour: A Multilevel Investigation in Three Countries
Authors: Laguna Mariola , Walachowska Karolina , Gorgievski-Duijvesteijn Marjan J. , Moriano Juan A.
Published: October 2019

The innovativeness of individual employees is a vital source of competitive advantage of firms, contributing to societal development. Therefore, the aim of this multilevel study was to examine how entrepreneurial firm owners’ authentic leadership relates to their employees’ innovative behaviour. Our conceptual model postulates that the relationship between business owners’ authentic leadership (as perceived by their employees) and their employees’ innovative behaviour is mediated by employees’ personal initiative and their work engagement. Hypotheses derived from this model were tested on data collected from 711 employees working in 85 small firms from three European countries: the Netherlands, Poland, and Spain. The results of the multilevel modelling confirmed our model, showing that when business owners are perceived as more authentic leaders, their employees show higher personal initiative and are more engaged at work and, in turn, identify more innovative solutions to be implemented in the organization. A cross-national difference was observed: employees from Spain (in comparison to Dutch and Polish employees) reported engaging less frequently in innovative behaviour. These research findings suggest that the innovative behaviour of employees can be boosted through leadership training, improving the quality of relationships between leaders and subordinates, and strengthening employees’ personal initiative and work engagement.
The Mediator and/or Moderator Role of Complexity of Knowledge about Healthy Eating and Self-Regulated Behavior on the Relation between Family’s Income and Children’s Obesity
Authors: Pereira Beatriz , Rosário Pedro , Silva Cátia , Figueiredo Gabriela , Núñez José Carlos , Magalhães Paula
Published: October 2019

Childhood obesity rates have been increasing over the years and is considered one of the most serious public health problems of the century. Low socioeconomic status has been associated with a higher body mass index. However, the pathways underlying this complex relationship are poorly understood. This study aimed to evaluate the possible mediation and/or moderation effects of complexity of knowledge about healthy eating, and self-regulation processes towards healthy eating, in the association between family’s income and weight of elementary school age children. The results showed that complexity of knowledge does not mediate the relationship between socioeconomic status and weight. Still, whenever the levels of complexity of knowledge and self-regulation are high, there is a moderation effect of complexity of knowledge on the relationship between family’s income and weight, conditioned by self-regulation scores. These promising findings support the idea that knowledge about healthy eating in isolation could be insufficient for practicing a healthy diet and underline the relevance of combining transmission of knowledge with training in specific competences (e.g., self-regulation strategies). Considering that complexity of knowledge about healthy eating and self-regulation strategies are interrelated and can be both improved, future preventive interventions could consider incorporating both in their design to mitigate childhood obesity.
Sexual Functioning in Pregnant Women
Authors: Fuchs Anna , Czech Iwona , Sikora Jerzy , Fuchs Piotr , Lorek Miłosz , Skrzypulec-Plinta Violetta , Drosdzol-Cop Agnieszka
Published: October 2019

Sexual activity during pregnancy is determined by emotional, psychosocial, hormonal, and anatomical factors and varies during trimesters. This work aimed to establish women’s sexual activity during each trimester of pregnancy. A total of 624 women were included in the study and filled in the questionnaire three times, once during each trimester of pregnancy. The first part of the survey included questions about socio-demographic characteristics, obstetric history, and medical details of a given pregnancy. The second part was the Polish version of the female sexual function index (FSFI) questionnaire. Comparison of the mean scores for the overall sexual function of each trimester revealed clinically relevant sexual dysfunction in the second and third trimesters (mean values 25.9 ± 8.7 and 22.7 ± 8.7, respectively; p < 0.01). Women were most sexually active during their second trimester. In the first trimester of pregnancy, women were most likely to choose intercourse in the missionary position. Women with vocational education were characterized by the lowest and homogenous FSFI values. Total FSFI score depended on the martial status—the highest value pertained to married women (25.2 ± 6.9; p = 0.02).
Seasonal Influenza Vaccination among Saudi Children: Parental Barriers and Willingness to Vaccinate Their Children
Authors: Alolayan Abdullah , Almotairi Bdoor , Alshammari Shouq , Alhearri Malak , Alsuhaibani Mohammed
Published: October 2019

Influenza is an acute respiratory infection. It is a contagious viral illness which can cause moderate to severe symptoms. However, high-risk groups, including children, can develop a severe condition requiring hospitalization that may, in severe cases, result in death. This study aimed to assess the knowledge and attitudes of Saudi parents toward the influenza vaccine and identify potential barriers to receiving the influenza vaccination. A cross-sectional survey was conducted using a questionnaire comprising 27 validated questions to assess parental awareness, knowledge, and attitudes toward the influenza vaccine. The overall attitude of the participants was positive (94.7%). However, their knowledge was generally poor (61.7%). Most participants were aware of the seasonal influenza vaccine (85.5%) and their children were up-to-date with the child national vaccination program vaccines (92.7%). Medical staff and awareness campaigns were the commonest sources of vaccine information. Significant predictors for knowledge about and attitudes toward the influenza vaccine included educational level, working in the medical field, monthly income, awareness of the seasonal influenza vaccine, having received the vaccine as parent, and having a child already vaccinated. Adherence to the influenza vaccination regimen for parents and their children was low. More educational campaigns are needed to increase knowledge about the vaccine.
Public Knowledge about Dementia in China: A National WeChat-Based Survey
Authors: Liu Dan , Cheng Guirong , An Lina , Gan Xuguang , Wu Yulian , Zhang Bo , Hu Sheng , Zeng Yan , Wu Liang
Published: October 2019

Dementia is a serious public health problem. The more extensive dementia knowledge is, the more conducive it is to early prevention and treatment of dementia. However, no assessment of the general population’s dementia awareness has been conducted so far in China. Thus, this study assessed the national public knowledge of dementia based on mobile internet in China. We assessed 10,562 national respondents recruited based on the most popular social networking service in China, WeChat and analyzed the data using quantitative methods. The overall correct rate of total dementia knowledge was 63.14%. Only half of the participants (50.84%) could identify risk factors accurately. The level of dementia knowledge was positively associated with high education, city residency, and experience of exposure to information on dementia. The sandwich generation (aged 20–60 years) had the highest level of dementia knowledge. Chinese people were found to have a low level of knowledge about dementia, especially those aged over 60 years, with low education and living in rural areas. Further educational programs and campaigns are needed to improve dementia knowledge, with greater focus on the older population as the target audience, emphasis on dementia risk factors as educational content, correcting misconceptions about dementia, and providing more experience of exposure to dementia.
A Study on Storm and Flood Insurance Management Mapping: Case Study of Incheon Metropolitan City
Authors: Lee Insu , Lee Junseok , Ham Sungil
Published: October 2019

In this research, we have used spatial information analysis techniques and procedures to process storm, flood, and snow damage risks, and apply premium rates to produce a Storm and Flood Insurance Management Map. To calculate risk, we used ArcGIS’s main features to overlay, integrate, and classify data. Moreover, we designed an ArcGIS Model Builder program to process very large amounts of risk data quickly and accurately. Excel’s pivot feature was used to calculate areas and premium rates according to flood depth. In the case of Incheon metropolitan city, the average risk was 2.85 on the 4-level scale, which lies between "alert" and "danger" and corresponds to the 1st of the 4 premium rate grades. In particular, there were high risks and high premium rates in areas around ports, ocean beaches, and beaches connected to rivers. We expect that this insurance management map created using spatial information analysis techniques will provide useful data for scientific natural disaster response and prevention planning, rational insurance rate calculation and application, and promotion of policies, which identify and prevent areas at risk for frequent storm and flood damage.
Structure and Junctional Complexes of Endothelial, Epithelial and Glial Brain Barriers
Authors: Castro Dias Mariana , Mapunda Josephine A. , Vladymyrov Mykhailo , Engelhardt Britta
Published: October 2019

The homeostasis of the central nervous system (CNS) is ensured by the endothelial, epithelial, mesothelial and glial brain barriers, which strictly control the passage of molecules, solutes and immune cells. While the endothelial blood-brain barrier (BBB) and the epithelial blood-cerebrospinal fluid barrier (BCSFB) have been extensively investigated, less is known about the epithelial and mesothelial arachnoid barrier and the glia limitans. Here, we summarize current knowledge of the cellular composition of the brain barriers with a specific focus on describing the molecular constituents of their junctional complexes. We propose that the brain barriers maintain CNS immune privilege by dividing the CNS into compartments that differ with regard to their role in immune surveillance of the CNS. We close by providing a brief overview on experimental tools allowing for reliable in vivo visualization of the brain barriers and their junctional complexes and thus the respective CNS compartments.
Liver X Receptors and Male (In)fertility
Authors: Jarvis Sheba , Williamson Catherine , Bevan Charlotte L
Published: October 2019

Liver X receptors (LXRs) are ligand-dependent transcription factors acting as ‘cholesterol sensors’ to regulate lipid homeostasis in cells. The two isoforms, LXRα (NR1H3) and LXRβ (NR1H2), are differentially expressed, with the former expressed predominantly in metabolically active tissues and the latter more ubiquitously. Both are activated by oxidised cholesterol metabolites, endogenously produced oxysterols. LXRs have important roles in lipid metabolism and inflammation, plus a number of newly emerging roles. They are implicated in regulating lipid balance in normal male reproductive function and may provide a link between male infertility and lipid disorders and/or obesity. Studies from Lxr knockout mouse models provide compelling evidence to support this. More recently published data suggest distinct and overlapping roles of the LXR isoforms in the testis and recent evidence of a role for LXRs in human male fertility. This review summarises the current literature and explores the likely link between LXR, lipid metabolism and male fertility as part of a special issue on Liver X receptors in International Journal of Molecular Sciences.
Vitamin E but Not GSH Decreases Reactive Oxygen Species Accumulation and Enhances Sperm Production during In Vitro Maturation of Frozen-Thawed Prepubertal Mouse Testicular Tissue
Authors: Arkoun Brahim , Galas Ludovic , Dumont Ludovic , Rives Aurélie , Saulnier Justine , Delessard Marion , Rondanino Christine , Rives Nathalie
Published: October 2019

Freezing–thawing procedures and in vitro culture conditions are considered as a source of stress associated with increased reactive oxygen species (ROS) generation, leading to a damaged cell aerobic metabolism and consequently to oxidative stress. In the present study, we sought to investigate whether vitamin E (Vit E) or reduced glutathione (GSH) enhances sperm production by decreasing ROS accumulation during in vitro maturation of prepubertal mice testes. Testes of prepubertal mice were cryopreserved using a freezing medium supplemented or not supplemented with Vit E and were cultured after thawing. In presence of Rol alone in culture medium, frozen-thawed (F-T) testicular tissues exhibited a higher ROS accumulation than fresh tissue during in vitro culture. However, Vit E supplementation in freezing, thawing, and culture media significantly decreased cytoplasmic ROS accumulation in F-T testicular tissue during in vitro maturation when compared with F-T testicular tissue cultured in the presence of Rol alone, whereas GSH supplementation in culture medium significantly increased ROS accumulation associated with cytolysis and tissue disintegration. Vit E but not GSH promoted a better in vitro sperm production and was a suitable ROS scavenger and effective molecule to improve the yield of in vitro spermatogenesis from F-T prepubertal mice testes. The prevention of oxidative stress in the cytoplasmic compartment should be regarded as a potential strategy for improving testicular tissue viability and functionality during the freeze–thaw procedure and in vitro maturation.
RETRACTED: Intermittent Hypoxia-Hyperoxia Training Improves Cognitive Function and Decreases Circulating Biomarkers of Alzheimer’s Disease in Patients with Mild Cognitive Impairment: A Pilot Study
Authors: Serebrovska Zoya , Serebrovska Tetiana , Kholin Viktor , Tumanovska Lesya , Shysh Angela , Pashevin Denis , Goncharov Sergii , Stroy Dmytro , Grib Oksana , Shatylo Valeriy , Bachinskaya Natalia , Egorov Egor , Xi Lei , Dosenko Victor
Published: October 2019

Alzheimer’s disease (AD) affects not only the central nervous system, but also peripheral blood cells including neutrophils and platelets, which actively participate in pathogenesis of AD through a vicious cycle between platelets aggregation and production of excessive amyloid beta (Aβ). Platelets adhesion on amyloid plaques also increases the risk of cerebral microcirculation disorders. Moreover, activated platelets release soluble adhesion molecules that cause migration, adhesion/activation of neutrophils and formation of neutrophil extracellular traps (NETs), which may damage blood brain barrier and destroy brain parenchyma. The present study examined the effects of intermittent hypoxic-hyperoxic training (IHHT) on elderly patients with mild cognitive impairment (MCI), a precursor of AD. Twenty-one participants (age 51–74 years) were divided into three groups: Healthy Control (n = 7), MCI+Sham (n = 6), and MCI+IHHT (n = 8). IHHT was carried out five times per week for three weeks (total 15 sessions). Each IHHT session consisted of four cycles of 5-min hypoxia (12% FIO2) and 3-min hyperoxia (33% FIO2). Cognitive parameters, Aβ and amyloid precursor protein (APP) expression, microRNA 29, and long non-coding RNA in isolated platelets as well as NETs in peripheral blood were investigated. We found an initial decline in cognitive function indices in both MCI+Sham and MCI+IHHT groups and significant correlations between cognitive test scores and the levels of circulating biomarkers of AD. Whereas sham training led to no change in these parameters, IHHT resulted in the improvement in cognitive test scores, along with significant increase in APP ratio and decrease in Aβ expression and NETs formation one day after the end of three-week IHHT. Such effects on Aβ expression and NETs formation remained more pronounced one month after IHHT. In conclusion, our results from this pilot study suggested a potential utility of IHHT as a new non-pharmacological therapy to improve cognitive function in pre-AD patients and slow down the development of AD.
Genome-Wide Identification of Long Non-coding RNA in Trifoliate Orange (Poncirus trifoliata (L.) Raf) Leaves in Response to Boron Deficiency
Authors: Zhou Gao-Feng , Zhang Li-Ping , Li Bi-Xian , Sheng Ou , Wei Qing-Jiang , Yao Feng-Xian , Guan Guan , Liu Gui-Dong
Published: October 2019

Long non-coding RNAs (lncRNAs) play important roles in plant growth and stress responses. As a dominant abiotic stress factor in soil, boron (B) deficiency stress has impacted the growth and development of citrus in the red soil region of southern China. In the present work, we performed a genome-wide identification and characterization of lncRNAs in response to B deficiency stress in the leaves of trifoliate orange (Poncirus trifoliata), an important rootstock of citrus. A total of 2101 unique lncRNAs and 24,534 mRNAs were predicted. Quantitative real-time polymerase chain reaction (qRT-PCR) experiments were performed for a total of 16 random mRNAs and lncRNAs to validate their existence and expression patterns. Expression profiling of the leaves of trifoliate orange under B deficiency stress identified 729 up-regulated and 721 down-regulated lncRNAs, and 8419 up-regulated and 8395 down-regulated mRNAs. Further analysis showed that a total of 84 differentially expressed lncRNAs (DELs) were up-regulated and 31 were down-regulated, where the number of up-regulated DELs was 2.71-fold that of down-regulated. A similar trend was also observed in differentially expressed mRNAs (DEMs, 4.21-fold). Functional annotation of these DEMs was performed using Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses, and the results demonstrated an enrichment of the categories of the biosynthesis of secondary metabolites (including phenylpropanoid biosynthesis/lignin biosynthesis), plant hormone signal transduction and the calcium signaling pathway. LncRNA target gene enrichment identified several target genes that were involved in plant hormones, and the expression of lncRNAs and their target genes was significantly influenced. Therefore, our results suggest that lncRNAs can regulate the metabolism and signal transduction of plant hormones, which play an important role in the responses of citrus plants to B deficiency stress. Co-expression network analysis indicated that 468 significantly differentially expressed genes may be potential targets of 90 lncRNAs, and a total of 838 matched lncRNA-mRNA pairs were identified. In summary, our data provides a rich resource of candidate lncRNAs and mRNAs, as well as their related pathways, thereby improving our understanding of the role of lncRNAs in response to B deficiency stress, and in symptom formation caused by B deficiency in the leaves of trifoliate orange.
The Gas-Phase Formation Mechanism of Dibenzofuran (DBF), Dibenzothiophene (DBT), and Carbazole (CA) from Benzofuran (BF), Benzothiophene (BT), and Indole (IN) with Cyclopentadienyl Radical
Authors: Li Xuan , Gao Yixiang , Zuo Chenpeng , Zheng Siyuan , Xu Fei , Sun Yanhui , Zhang Qingzhu
Published: October 2019

Benzofuran (BF), benzothiophene (BT), indole (IN), dibenzofuran (DBF), dibenzothiophene (DBT), and carbazole (CA) are typical heterocyclic aromatic compounds (NSO-HETs), which can coexist with polycyclic aromatic hydrocarbons (PAHs) in combustion and pyrolysis conditions. In this work, quantum chemical calculations were carried out to investigate the formation of DBF, DBT, and CA from the reactions of BF, BT, and IN with a cyclopentadienyl radical (CPDyl) by using the hybrid density functional theory (DFT) at the MPWB1K/6-311+G(3df,2p)//MPWB1K/6-31+G(d,p) level. The rate constants of crucial elementary steps were deduced over 600−1200 K, using canonical variational transition state theory with a small-curvature tunneling contribution (CVT/SCT). This paper showed that the production of DBF, DBT, and CA from the reactions of BF, BT, and IN with CPDyl involved six elementary steps: the addition reaction, ring closure, the first H shift, C–C cleavage, the second H shift, and elimination of CH3 or H. The cleavage of the C–C bond was regarded as the rate-determining step for each pathway due to the extremely high barrier. The 1-methyl substituted products were more easily formed than the 4-methyl substituted products. The main products were DBF and 1-methyl-DBF, DBT and 1-methyl-DBT, and CA and 1-methyl-CA for reactions of BF, BT, and IN with CPDyl, respectively. The ranking of DBF, DBT, and CA formation potential was as follows: DBT and methyl-DBT formation > DBF and methyl-DBF formation > CA, and methyl-CA formation. Comparison with the reaction of naphthalene with CPDyl indicated that the reactions of CPDyl attacking a benzene ring and a furan/thiophene/pyrrole ring could be inferred to be comparable under high temperature conditions.
EPO and TMBIM3/GRINA Promote the Activation of the Adaptive Arm and Counteract the Terminal Arm of the Unfolded Protein Response after Murine Transient Cerebral Ischemia
Authors: Habib Pardes , Stamm Ann-Sophie , Schulz Joerg B. , Reich Arno , Slowik Alexander , Capellmann Sandro , Huber Michael , Wilhelm Thomas
Published: October 2019

Ischemic stroke is known to cause the accumulation of misfolded proteins and loss of calcium homeostasis leading to impairment of endoplasmic reticulum (ER) function. The unfolded protein response (UPR) is an ER-located and cytoprotective pathway that aims to resolve ER stress. Transmembrane BAX inhibitor-1 motif-containing (TMBIM) protein family member TMBIM3/GRINA is highly expressed in the brain and mostly located at the ER membrane suppressing ER calcium release by inositol-1,4,5-trisphosphate receptors. GRINA confers neuroprotection and is regulated by erythropoietin (EPO) after murine cerebral ischemia. However, the role of GRINA and the impact of EPO treatment on the post-ischemic UPR have not been elucidated yet. We subjected GRINA-deficient (Grina−/−) and wildtype mice to transient (30 min) middle cerebral artery occlusion (tMCAo) followed by 6 h or 72 h of reperfusion. We administered EPO or saline 0, 24 and 48 h after tMCAo/sham surgery. Oxygen–glucose deprivation (OGD) and pharmacological stimulation of the UPR using Tunicamycin and Thapsigargin were carried out in primary murine cortical mixed cell cultures. Treatment with the PERK-inhibitor GSK-2606414, IRE1a-RNase-inhibitor STF-083010 and EPO was performed 1 h prior to either 1 h, 2 h or 3 h of OGD. We found earlier and larger infarct demarcations in Grina−/− mice compared to wildtype mice, which was accompanied by a worse neurological outcome and an abolishment of EPO-mediated neuroprotection after ischemic stroke. In addition, GRINA-deficiency increased apoptosis and the activation of the corresponding PERK arm of the UPR after stroke. EPO enhanced the post-ischemic activation of pro-survival IRE1a and counteracted the pro-apoptotic PERK branch of the UPR. Both EPO and the PERK-inhibitor GSK-2606414 reduced cell death and regulated Grina mRNA levels after OGD. In conclusion, GRINA plays a crucial role in post-ischemic UPR and the use of both GSK-2606414 and EPO might lead to neuroprotection.