bluuebunny/crossref_metadata_2025 huggingface dataset:
Repellency, Fumigant Toxicity, Antifeedent and Residual Activities of Coridothymus capitatus and Its Main Component Carvacrol against Red Flour Beetle
Authors: Eltalawy Hassan M. , El-Fayoumi Huda , Aboelhadid Shawky M. , Al-Quraishy Saleh , El-Mallah Almahy M. , Tunali Fatma , Sokmen Atalay , Daferera Dimitra , Abdel-Baki Abdel-Azeem S.
Published: September 2024

Tribolium castaneum is a challenging pest of stored products, causing significant economic losses. The present study explored the efficacy of Coridothymus capitatus essential oil and its primary constituent, carvacrol, as eco-friendly alternatives for managing this pest. To evaluate their insecticidal potential, repellency, fumigant toxicity, and antifeedant properties, progeny inhibition assays were performed. Carvacrol exhibited superior repellency compared to the essential oil, achieving a 92% repellency rate at 2 mg/cm2. Both compounds demonstrated significant fumigant toxicity against T. castaneum, with LC50 values of 168.47 and 106.5 μL/L for the essential oil and carvacrol, respectively, after 24 h. Carvacrol also outperformed the essential oil in antifeedant activity, inducing an 80.7% feeding deterrence at 1.17 mg/g. Moreover, both treatments effectively suppressed the development of the pest’s progeny. These results collectively underscore the potent insecticidal properties of C. capitatus essential oil and carvacrol, particularly carvacrol, as promising candidates for the sustainable management of T. castaneum in stored product protection.
Advances in Electrospun Nanofiber Membranes for Dermatological Applications: A Review
Authors: Han Yuanyuan , Wei Hewei , Ding Qiteng , Ding Chuanbo , Zhang Shuai
Published: September 2024

In recent years, a wide variety of high-performance and versatile nanofiber membranes have been successfully created using different electrospinning methods. As vehicles for medication, they have been receiving more attention because of their exceptional antibacterial characteristics and ability to heal wounds, resulting in improved drug delivery and release. This quality makes them an appealing choice for treating various skin conditions like wounds, fungal infections, skin discoloration disorders, dermatitis, and skin cancer. This article offers comprehensive information on the electrospinning procedure, the categorization of nanofiber membranes, and their use in dermatology. Additionally, it delves into successful case studies, showcasing the utilization of nanofiber membranes in the field of skin diseases to promote their substantial advancement.
Synthesis and Anticancer Activity of 3,4-Diaryl-1,2-dihydro- and 1,2,3,4-Tetrahydroquinolines
Authors: Rajput Santosh , Falasca Valerio , Bhadbhade Mohan , Black David StC , Kumar Naresh
Published: September 2024

Tetrahydroquinolines are key structures in a variety of natural products with diverse pharmacological utilities and other applications. A series of 3,4-diaryl-5,7-dimethoxy-1,2,3,4-tetrahydroquinolines were synthesized in good yield by reacting 3-aryl-5,7-dimethoxy-2,3-dihydroquinolin-4-ones with different Grignard reagents followed by the dehydration of the intermediate phenolic compounds. Subsequent reduction and deprotection were carried out to achieve the desired tetrahydroquinolone moiety. The lead compound 3c showed low micromolar inhibition of various cancer cell lines. Demethylation under different reaction conditions was also investigated to afford the corresponding monohydroxy analogues.
Reverse Mode Polymer Stabilized Cholesteric Liquid Crystal Flexible Films with Excellent Bending Resistance
Authors: Yu Ping , He Zemin , Zhao Yuzhen , Song Wenqi , Miao Zongcheng
Published: September 2024

The reverse-mode smart windows, which usually fabricated by polymer stabilized liquid crystal (PSLC), are more practical for scenarios where high transparency is a priority for most of the time. However, the polymer stabilized cholesteric liquid crystal (PSCLC) film exhibits poor spacing stability due to the mobility of CLC molecules during the bending deformation. In this work, a reverse-mode PSCLC flexible film with excellent bending resistance was fabricated by the construction of polymer spacer columns. The effect of the concentration of the polymerizable monomer C6M and chiral dopant R811 on the electro-optical properties and polymer microstructure of the film were studied. The sample B2 containing 3 wt% of C6M and 3 wt% R811 presented the best electro-optical performance. The electrical switch between transparent and opaque state of the flexible PSCLC film after bending not only indicated the excellent electro-optical switching performance, but also demonstrated the outstanding bending resistance of the sample with polymer spacer columns, which makes the PSCLC film containing polymer spacer columns have a great potential to be applied in the field of flexible devices.
FAMS—A Targeted Fatty Acid Mass Spectrometry Method for Monitoring Free Fatty Acids from Polysorbate Hydrolysis
Authors: Bathke Anja , Hoelterhoff Sina , Wendler Jan , Yuk Inn H. , Bell Christian H.
Published: September 2024

Polysorbates are the predominant surfactants used to stabilize protein formulations. Unfortunately, polysorbates can undergo hydrolytic degradation, which releases fatty acids that can accumulate to form visible particles. The detection and quantitation of these fatty acid degradation products are critical for assessing the extent of polysorbate degradation and the associated risks of particle formation. We previously developed a user-friendly mass spectrometric method called Fatty Acids by Mass Spectrometry (FAMS) to quantify the free fatty acids. The FAMS method was validated according to ICH Q2 (R1) guidelines and is suitable for a wide range of products, buffers and protein concentrations. The end-to-end workflow can be automated from sample preparation to data analysis. To broaden method accessibility, the QDa detector selected for fatty acid measurement does not require specific mass spectrometry experience. We provide here a detailed procedure for both manual and automated sample preparation for high-throughput analysis. In addition, we highlight in this protocol the critical operational details, procedural watchouts and troubleshooting tips to support the successful execution of this method in another laboratory.
Engineering Moderately Lithiophilic Paper-Based Current Collectors with Variable Solid Electrolyte Interface Films for Anode-Free Lithium Batteries
Authors: Yang Baohong , Wei Hairu , Wang Huan , Wu Haoteng , Guo Yanbo , Ren Xuan , Xiong Chuanyin , Liu Hanbin , Wu Haiwei
Published: September 2024

Compared to traditional lithium metal batteries, anode-free lithium metal batteries use bare current collectors as an anode instead of Li metal, making them highly promising for mass production and achieving high-energy density. The current collector, as the sole component of the anode, is crucial in lithium deposition-stripping behavior and greatly impacts the rate of Li depletion from the cathode. In this study, to investigate the lithiophilicity effect of the current collector on the solid electrolyte interface (SEI) film construction and cycling performance of anode-free lithium batteries, various lightweight paper-based current collectors were prepared by electroless plating Cu and lipophilic Ag on low-dust paper (LDP). The areal densities of the as-prepared LDP@Cu, LDP@Cu-Ag, and LDP@Ag were approximately 0.33 mg cm−2. The use of lipophilic Ag-coated collectors with varying loadings allowed for the regulation of lipophilicity. The impacts of these collectors on the distribution of SEI components and Li depletion rate in common electrolytes were investigated. The findings suggest that higher loadings of lipophilic materials, such as Ag, on the current collector increase its lipophilicity but also lead to significant Li depletion during the cycling process in full-cell anode-free Li metal batteries. Thus, moderately lithiophilic current collectors, such as LDP@Cu-Ag, show more potential for Li deposition and striping and stable SEI with a low speed of Li depletion.
Structural Health Monitoring of Glass Fiber-Reinforced Polymer Laminates with Carbon Nanotube-Coated Glass Fiber Sensing Layer after Low-Velocity Impact Using Electrical Resistance Tomography
Authors: Zhao Zijie , Li Minglong , Liu Ya , Wang Anhua , Zhou Biaojun , Hu Junfeng
Published: September 2024

Structural health monitoring (SHM) of composite materials is of great significance in various practical applications. However, it is a challenge to accurately monitor the damage of composites without affecting their mechanical properties. In this paper, an embedded sensing layer based on carbon nanotube-coated glass fiber is designed, combined with electrical resistance tomography (ERT) for in situ damage monitoring. Multi-wall carbon nanotube-coated glass fiber (MWCNT-GF) is prepared and embedded into laminates as an in situ sensing layer. Low-velocity impact experiments demonstrate that the embedded sensing layer has high compatibility with the composite laminates and has no adverse effect on its impact response; although, the energy absorption behavior of glass fiber-reinforced polymer (GFRP) laminates containing MWCNT-GF occurs about 10% earlier than that of GFRP laminates overall. ERT technology is used to analyze the laminates after a low-velocity impact test. The results show that the in situ monitoring method with the embedded MWCNT-GF sensing layer can achieve high precision in imaging localization of impact damage, and the error of the detected damage area is only 4.5%.
Cerebrovascular Reactivity Assessed by Breath-Hold Functional MRI in Patients with Neurological Post-COVID-19 Syndrome—A Pilot Study
Authors: Zerweck Leonie , Klose Uwe , Mengel Annerose , Hoheisel Tobias , Eikemeier Melinda , Richter Vivien , Joos Natalie Sophie , Ernemann Ulrike , Bender Benjamin , Hauser Till-Karsten
Published: September 2024

Endothelial dysfunction represents a potential pathomechanism of neurological post-COVID-19 syndrome (PCS). A recent study demonstrated reduced cerebrovascular reactivity (CVR) in patients with PCS. The aim of this pilot study was to prospectively assess CVR in patients with PCS using breath-hold functional MRI (bh-fMRI). Fourteen patients with neurological PCS and leading symptoms of fatigue/memory issues/concentration disorder (PCSfmc), 11 patients with PCS and leading symptoms of myopathy/neuropathy (PCSmn), and 17 healthy controls underwent bh-fMRI. Signal change and time to peak (TTP) were assessed globally and in seven regions of interest and compared between the subgroups using one-way ANCOVA adjusting for age, time since infection, Fazekas score, and sex. No significant differences were observed. In PCS patients, the global CVR exhibited a slight, non-significant tendency to be lower compared to healthy controls (PCSfmc: 0.78 ± 0.11%, PCSmn: 0.84 ± 0.10% and 0.87 ± 0.07%). There was a non-significant trend towards lower global TTP values in the PCS subgroups than in the control group (PCSfmc: 26.41 ± 1.39 s, PCSmn: 26.32 ± 1.36 s versus 29.52 ± 0.93 s). Endothelial dysfunction does not seem to be the sole pathomechanism of neurological symptoms in PCS. Further studies in larger cohorts are required.
Obesity Prevention Effects of Avocado (Persea americana) Seed Powder in High-Fat Diet-Induced Obesity in Rats
Authors: Mokhele Shoeshoe , Aboyade Oluwaseyi , Katerere David R.
Published: September 2024

Avocado seed contains 64% of the phenolic compounds of the whole fruit. This makes avocado seed a potential candidate for the development of treatments for different illnesses, including obesity (the major risk factor for metabolic disorders). The aim of this study was to investigate the effects of avocado seed powder on high-fat diet-induced obesity in rats. Sprague Dawley rats (16 rats) were fed a high-fat diet for 10 weeks. After 10 weeks, the rats were assigned into two groups of eight animals each and were fed either a high-fat diet (HFD; control group) or a high-fat diet containing avocado seed powder (HFD-A; treatment group) for 6 weeks. Animals were weighed weekly, and weekly weight gain was determined. Animals in the treatment (avocado seed) group showed significantly lower body weight gain (7.8 ± 9.63 g) than animals in the control group (33.9 ± 10.84 g) at the end of this study. The treatment group presented with lower triglycerides than the control, with LDL and HDL comparable to the control group. Avocado seed powder showed potential to reduce obesity in rats fed a high-fat diet. Avocado seed can therefore be investigated further as a potential anti-obesity nutraceutical.
Anthrax in Humans, Animals, and the Environment and the One Health Strategies for Anthrax Control
Authors: Subedi Deepak , Pantha Saurav , Jyoti Sumit , Gautam Bickal , Kaphle Krishna , Yadav Rakesh Kumar , Ghimire Shristi , Dhakal Santosh
Published: September 2024

Anthrax is a notorious disease of public health importance caused by Bacillus anthracis. The causative agent can also be used as a biological weapon. Spores of these bacteria can sustain extreme environmental conditions and remain viable in soil for decades. Domestic and wild ruminants are highly susceptible to this pathogen, which usually presents as a peracute to acute disease. In humans, cutaneous anthrax is frequent but pulmonary and enteric anthrax are more serious. Humans, animals, and the environment are all involved, making anthrax a perfect target for a One Health approach. The environment plays a key role in disease transmission. At a time when the One Health concept is not mere slogans, collaborative efforts of medical professionals, veterinarians, and environmental scientists will be valuable for the prevention and control of this disease. In this review, we discussed the transmission dynamics of anthrax in the environment, animals, and humans, as well as One Health strategies to control and prevent anthrax.
Innovative Multiplex PCR Assay for Detection of tlh, trh, and tdh Genes in Vibrio parahaemolyticus with Reference to the U.S. FDA’s Bacteriological Analytical Manual (BAM)
Authors: Park Seong Bin , Zhang Yan
Published: September 2024

Vibrio parahaemolyticus is an important foodborne bacterium that causes severe gastroenteritis following the consumption of contaminated seafood. To identify V. parahaemolyticus and determine its pathogenicity, the U.S. Food and Drug Administration (FDA)’s Bacteriological Analytical Manual (BAM) recommends a multiplex polymerase chain reaction (PCR) protocol to simultaneously detect the species-specific thermolabile hemolysin (tlh) gene and the pathogenic thermostable-related hemolysin (trh) and thermostable-direct hemolysin (tdh) genes. However, this assay has shown two limitations: difficulty in separating the amplicons of the trh (486 bp) and tlh (450 bp) genes due to their highly similar sizes, and the weaker band exhibited by the tdh gene amplicon (270 bp). The present study aimed to improve the BAM’s multiplex PCR assay by separating the three amplicons with similar intensity. A new primer set was applied for the tlh gene (369 bp) alongside the existing primers for the trh and tdh genes. The amplicons for the three genes were effectively separated by electrophoresis on a 2% tris-borate-EDTA (TBE) agarose gel within 45 min. Primer concentrations of 0.25 µM for three genes produced a significant amount of amplicons among various combinations of primer concentrations with 35 PCR cycles. This assay exhibited a detection limit of 10 pg of bacterial DNA, demonstrating its high sensitivity. It did not display amplicons from nine Vibrio species known to be human pathogens or from 18 well-documented foodborne pathogens. Therefore, the present multiplex PCR protocol could help overcome the limitations of existing assays and provide a more reliable method for detecting the three genes of V. parahaemolyticus.
Lipid-Based Gels for Delivery of 3-O-Ethyl L-Ascorbic acid in Topical Applications
Authors: Loza-Rodríguez Noèlia , Millán-Sánchez Aina , Mallandrich Mireia , Calpena Ana Cristina , López Olga
Published: September 2024

This study explores the incorporation of 10% 3-O-ethyl L-ascorbic acid (ETVC), a derivative of vitamin C, into two lipid gel systems: a hydrogel (HG) consisting exclusively of lipids and water and a bigel (BG) combining the hydrogel with an oleogel made from olive oil and beeswax. We investigated the ETVC release profiles from both materials using synthetic membranes and measured their permeation through porcine skin in vitro. Additionally, the interaction of these lipid gel systems with the stratum corneum (SC) was determined. Results from the release study indicate that the BG exhibited slower ETVC release compared to the HG. The permeation experiments showed that the presence of lipids in the formulations enhanced ETVC retention in the skin. The HG delivered a higher amount to the SC, while the BG achieved greater retention in the epidermis. This difference is attributed to the different lipophilic nature of each material. The structural analysis of SC lipids revealed that the organization of surface lipids remained unaltered by the application of the gels. Finally, an in vitro efficacy test in porcine skin using methylene blue indicated that our ETVC gels exhibited antioxidant activity. These findings provide valuable insights into the potential of lipid-based gels for topical applications.
Synergistic Chemo-Immunotherapy: Recombinant Fusion Protein-Based Surface Modification of NK Cell for Targeted Cancer Treatment
Authors: Lim Su Yeon , Kim Luna , Kim Hongbin , Park Jeong-Ann , Yun Jina , Lim Kwang Suk
Published: September 2024

While traditional combination anticancer treatments have shown promising results, there remains significant interest in developing innovative methods to enhance and integrate chemotherapy and immunotherapy. This study introduces a recombinant fusion protein-based cell surface modification system that synergistically combines chemotherapy and immunotherapy into a single-targeted chemo-immunotherapy approach. A cell surface-modified protein composed of an antibody-specific binding domain and a cell-penetrating domain rapidly converts immune cells into chemo-immuno therapeutics by binding to antibodies on the surface of immune cells. Utilizing a non-invasive, non-toxic approach free of chemical modifications and binding, our system homogeneously transforms immune cells by transiently introducing targeted cytotoxic drugs into them. The surface-engineered immune cells loaded with antibody–drug conjugates (ADCs) significantly inhibit the growth of target tumors and enhance the targeted elimination of cancer cells. Therefore, NK cells modified by the cell surface-modified protein to incorporate ADCs could be expected to achieve the combined effects of targeted cancer cell recognition, chemotherapy, and immunotherapy, thereby enhancing their therapeutic efficacy against cancer. This strategy allows for the efficient and rapid preparation of advanced chemo-immuno therapeutics to treat various types of cancer and provides significant potential to improve the efficacy of cancer treatment.
Binary and Ternary Inclusion Complexes of Niflumic Acid: Synthesis, Characterization, and Dissolution Profile
Authors: Bouchekhou Zohra , Hadj Ziane-Zafour Amel , Lupascu Florentina Geanina , Profire Bianca-Ștefania , Nicolescu Alina , Bostiog Denisse-Iulia , Doroftei Florica , Dascalu Ioan-Andrei , Varganici Cristian-Dragoș , Pinteala Mariana , Profire Lenuta , Pinteala Tudor , Bouzid Bachir
Published: September 2024

Although niflumic acid (NA) is one of the most used non-steroidal anti-inflammatory drugs, it suffers from poor solubility, low bioavailability, and significant adverse effects. To address these limitations, the complexation of NA with cyclodextrins (CDs) is a promising strategy. However, complexing CDs with low molecular weight drugs like NA can lead to low CE. This study explores the development of inclusion complexes of NA with 2-hydroxypropyl-β-cyclodextrin (2HP-β-CD), including the effect of converting NA to its sodium salt (NAs) and adding hydroxypropyl methylcellulose (HPMC) on complex formation. Inclusion complexes were prepared using co-evaporation solvent and freeze-drying methods, and their CE and Ks were determined through a phase solubility study. The complexes were characterized using physicochemical analyses, including FT-IR, DSC, SEM, XRD, DLS, UV-Vis, 1H-NMR, and 1H-ROESY. The dissolution profiles of the complexes were also evaluated. The analyses confirmed complex formation for all systems, demonstrating drug–cyclodextrin interactions, amorphous drug states, morphological changes, and improved solubility and dissolution profiles. The NAs-2HP-β-CD-HPMC complex exhibited the highest CE and Ks values, a 1:1 host-guest molar ratio, and the best dissolution profile. The results indicate that the NAs-2HP-β-CD-HPMC complex has potential for delivering NA, which might enhance its therapeutic effectiveness and minimize side effects.
Genome-Wide Analysis of the Nramp Gene Family in Kenaf (Hibiscus cannabinus): Identification, Expression Analysis, and Response to Cadmium Stress
Authors: Liu Qin , Li Shaocui , Du Guanghui , An Xia
Published: September 2024

Kenaf (Hibiscus cannabinu) is a grass bast fiber crop that has the ability to tolerate and accumulate heavy metals, and it has been considered as a potential heavy metal accumulator and remediation plant. Nramp is a natural resistance-related macrophage, which plays an important role in the transport of divalent metal ions, plant growth and development, and abiotic stress. In this study, the Nramp gene family of kenaf was analyzed at the whole genome level. A total of 15 HcNramp genes were identified. They are distributed unevenly on chromosomes. Phylogenetic analysis classified 15 HcNramp proteins into 3 different subfamilies. All proteins share specific motif 4 and motif 6, and the genes belonging to the same subfamily are similar in structure and motif. The promoters are rich in hormone response, meristem expression, and environmental stress response elements. Under different treatments, the expression levels of HcNramp genes vary in different tissues, and most of them are expressed in roots first. These findings can provide a basis for understanding the potential role of the Nramp gene family in kenaf in response to cadmium (Cd) stress, and are of great significance for screening related Cd tolerance genes in kenaf.
Antibacterial and Antioxidant Activities of Hydroalcoholic and Phenolic Extracts from Ternstroemia dentisepala and T. lineata Leaves
Authors: Díaz Alexis Uriel Soto , Villarreal María Luisa , Santos Marcelo Victorio-De los , Cardoso-Taketa Alexandre Toshirrico
Published: September 2024

Traditional Mexican medicine commonly uses infusions of Ternstroemia spp. to treat insomnia, injuries, and infections. The antibacterial activities of Ternstroemia dentisepala and Ternstroemia lineata were evaluated for the first time against a panel of Gram-positive and Gram-negative bacteria that have implications for human health, including Enterococcus faecalis, Streptococcus agalactiae, Streptococcus pyogenes, Salmonella typhi, Pseudomonas aeruginosa, and Vibrio parahaemolyticus. Furthermore, the scavenging potential of the hydroalcoholic (HAEs) and total phenolic extracts (TPEs) from the leaves of both plants by a 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) assay (ABTS•+) was determined. Also, the total phenolic contents of the HAEs using the Folin–Ciocalteu reagent were assayed. T. dentisepala HAE and TPE were active against all bacterial strains tested, with a minimum inhibitory concentration between 1.0 and 6.0 mg/mL, with the last one being the most active. However, the T. lineata extracts only demonstrated effectiveness against S. typhi and P. aeruginosa. The TPEs from T. dentisepala and T. lineata improved the activity by approximately 30% in all bacteria tested in comparison with the HAEs. The T. dentisepala HAE had a higher total phenolic content than the T. lineata extract, which was consistent with its ABTS•+-scavenging activity. The two HAEs had different chemical profiles, mostly because of the types and amounts of phenolic compounds they contained. These profiles were obtained using thin-layer chromatography (TLC), high-performance liquid chromatography (HPLC), and proton nuclear magnetic resonance (1H NMR) experiments.
Aminoethoxyvinylglicine and 1-Methylcyclopropene: Effects on Preharvest Drop, Fruit Maturity, Quality, and Associated Gene Expression of ‘Honeycrisp’ Apples in the US Mid-Atlantic
Authors: Johnson Emily , Farcuh Macarena
Published: September 2024

Preharvest fruit drop is one of the main challenges in apple production as it can lead to extensive crop losses in commercially important cultivars including ‘Honeycrisp’. Plant growth regulators, such as aminoethoxyvinylglicine (AVG) and 1-methylcyclopropene (1-MCP), which hinder ethylene biosynthesis and perception, respectively, can control preharvest fruit drop, but an assessment of their effects in ‘Honeycrisp’ fruit grown under US mid-Atlantic conditions is lacking. In this study, we evaluated the effects of AVG (130 mg a.i. L−1) and 1-MCP (150 mg a.i. L−1) on preharvest fruit drop, ethylene production, fruit physicochemical parameters, skin color, and transcript accumulation of ethylene and anthocyanin-related genes in ‘Honeycrisp’ apples throughout on-the-tree ripening. We showed that both AVG and 1-MCP significantly minimized preharvest fruit drop with respect to the control fruit. Additionally, AVG was the most effective in decreasing ethylene production, downregulating ethylene biosynthesis and perception-related gene expression, and delaying fruit maturity. Nevertheless, AVG negatively impacted apple red skin color and exhibited the lowest expression of anthocyanin-biosynthesis-related genes, only allowing apples to reach the minimum required 50% blush at the last ripening stage. Conversely, 1-MCP-treated fruit displayed an intermediate behavior between AVG-treated and control fruit, decreasing ethylene production rates and the associated gene expression as well as delaying fruit maturity when compared to the control fruit. Remarkably, 1-MCP treatment did not sacrifice red skin color development or anthocyanin-biosynthesis-related gene expression, thus exhibiting > 50% blush one week earlier than AVG.
Microplastic and Nanoplastic in Crops: Possible Adverse Effects to Crop Production and Contaminant Transfer in the Food Chain
Authors: Jadhav Bhakti , Medyńska-Juraszek Agnieszka
Published: September 2024

With the increasing amounts of microplastic (MP) deposited in soil from various agricultural activities, crop plants can become an important source of MP in food products. The last three years of studies gave enough evidence showing that plastic in the form of nanoparticles (<100 nm) can be taken up by the root system and transferred to aboveground plant parts. Furthermore, the presence of microplastic in soil affects plant growth disturbing metabolic processes in plants, thus reducing yields and crop quality. Some of the adverse effects of microplastic on plants have been already described in the meta-analysis; however, this review provides a comprehensive overview of the latest findings about possible adverse effects and risks related to wide microplastic occurrence in soil on crop production safety, including topics related to changes of pesticides behavior and plant pathogen spreading under the presence MP and possibly threaten to human health.
Lobelia Lakes’ Vegetation and Its Photosynthesis Pathways Concerning Water Parameters and the Stable Carbon Isotopic Composition of Plants’ Organic Matter
Authors: Pronin Eugeniusz , Banaś Krzysztof , Chmara Rafał , Ronowski Rafał , Merdalski Marek , Santoni Anne-Lise , Mathieu Olivier
Published: September 2024

Most of the aquatic vegetation produces organic substances via the C3 photosynthetic pathway (mosses, isoetids—Lobelia dortmanna L., Luronium natans (L.) Raf., and vascular plants) or Crassulacean acid metabolism (CAM, e.g., Littorella uniflora (L.) Asch. and Isoëtes lacustris L.) or by their ability to use HCO3− via carbon concentration mechanisms (CCMs—some elodeids and charophytes). Differentiating these predominant photosynthetic pathways in aquatic vegetation based on their organic matter (OM) carbon stable isotopes (δ13CORG) is a complex task, in contrast to terrestrial plants. This study investigates the OM deposition, characterized by δ13CORG values in 10 macrophyte species with different photosynthetic pathways (C3, CAM, and CCM) collected from 14 softwater Lobelia lakes in northern Poland. The higher δ13CORG values distinguish the CCM group, indicating their use of 13C-enriched HCO3¯ in photosynthesis. CAM species show slightly higher δ13CORG values than C3, particularly in lower pH lakes. Principal component analysis of isotopic and environmental data did not yield clear distinctions by the groups, but still, they significantly differ in light of analyzed parameters and isotopic signals (PRMANOVA = 5.08, p < 0.01; K-W H = 27.01, p < 0.001). The first two PCA dimensions showed that the water pH and Ca2+ concentration positively influenced δ13C values. The influence of light conditions on δ13CORG values revealed by third PCA components seems to also be important. In summary, northern Polish Lobelia lakes serve as a key differentiation point between vegetation employing CCMs and those relying on C3/CAM photosynthesis without HCO3− utilization, providing insights into transitions in plant communities within these ecosystems.
Identification of Insertion and Deletion (InDel) Markers for Chickpea (Cicer arietinum L.) Based on Double-Digest Restriction Site-Associated DNA Sequencing
Authors: Sari Duygu
Published: September 2024

Enhancing the marker repository and the development of breeder-friendly markers in chickpeas is important in relation to chickpea genomics-assisted breeding applications. Insertion–deletion (InDel) markers are widely distributed across genomes and easily observed with specifically designed primers, leading to less time, cost, and labor requirements. In light of this, the present study focused on the identification and development of InDel markers through the use of double-digest restriction site-associated DNA sequencing (ddRADSeq) data from 20 chickpea accessions. Bioinformatic analysis identified 20,700 InDel sites, including 15,031 (72.61%) deletions and 5669 (27.39%) insertions, among the chickpea accessions. The InDel markers ranged from 1 to 25 bp in length, while single-nucleotide-length InDel markers were found to represent the majority of the InDel sites and account for 79% of the total InDel markers. However, we focused on InDel markers wherein the length was greater than a single nucleotide to avoid any read or alignment errors. Among all of the InDel markers, 96.1% were less than 10 bp, 3.6% were between 10 and 20 bp, and 0.3% were more than 20 bp in length. We examined the InDel markers that were 10 bp and longer for the development of InDel markers based on a consideration of the genomic distribution and low-cost genotyping with agarose gels. A total of 29 InDel regions were selected, and primers were successfully designed to evaluate their efficiency. Annotation analysis of the InDel markers revealed them to be found with the highest frequency in the intergenic regions (82.76%), followed by the introns (6.90%), coding sequences (6.90%), and exons (3.45%). Genetic diversity analysis demonstrated that the polymorphic information content of the markers varied from 0.09 to 0.37, with an average of 0.20. Taken together, these results showed the efficiency of InDel marker development for chickpea genetic and genomic studies using the ddRADSeq method. The identified markers might prove valuable for chickpea breeders.
Generic Workflow of a Highly Effective and Easy Anther Culture Method for Both Japonica and Indica Rice
Authors: Guo Guimei , Liu Shisen , Zhang Shuwei , Yang Linian , Zong Yingjie , Halford Nigel G. , He Ting , Gao Runhong , Guo Zhenzhu , Zhou Longhua , Liu Chenghong , Wu Shujun , Chen Zhiwei
Published: September 2024

As one of the most important staple crops in the world, rice plays a pivotal role in world food security. The creation of doubled haploids based on anther culture is an important technology for rice breeding. However, at present, rice anther culture technology still faces many problems, such as genotype dependency, especially genotypes of indica rice. In this study, fifteen rice genotypes, including twelve japonica rice genotypes and three indica rice genotypes, were randomly selected and used to study anther culture by using a modified M8 medium. The results showed that the total callus induction rates of these different rice genotypes ranged from 0.81 to 13.95%, with an average of 6.64%, while the callus induction rates calculated for the top ten highest callus inductions for each rice genotype ranged from 2.75 to 17.00%, with an average of 10.56%. There were varying gaps between the total callus induction rates and the callus induction rates in these different rice genotypes. The fact that the gaps for some rice genotypes were relatively large indicated that standard tiller or anther collection was not applicable to all rice genotypes and that there was still a lot of room for improvement in the callus induction rate of some rice genotypes through optimization of the sampling method. The plantlet regeneration rates ranged from 12.55 to 456.54%, with an average of 200.10%. Although there were many albinos from anther culture for some rice genotypes, these would still meet the requirement if the rice genotypes had higher callus induction rates or regeneration rates. The percentages of seed setting of regenerated green seedlings ranged from 14% to 84%, with an average of 48.73%. Genetic diversity analysis showed that the genetic background of these different rice genotypes was representative, and the phylogenetic tree and Principal Component Analysis (PCA) divided them into indica and japonica types. Therefore, in this study, an anther culture method suitable for both indica and japonica rice genotypes was established, which could improve doubled haploid breeding in rice.
Dentatacid A: An Unprecedented 2, 3-Seco-arbor-2, 3-dioic Triterpenoid from the Invasive Plant Euphorbia dentata, with Cytotoxicity Effect on Colon Cancer
Authors: Xu Chen-Sen , Shao Yuan-Ling , Li Qing , Zhang Yu , Wu Hong-Wei , Yu Hao-Lin , Su Yun-Yun , Zhang Jing , Wang Chao , Liao Zhi-Xin
Published: September 2024

Euphorbia dentata Michx. is an invasive plant species in China, known for its toxicity and potential to reduce crop yields, posing numerous threats. To gain a deeper understanding of this invasive plant, phytochemical methods were employed to isolate 13 terpenoids (1–11, 19, 20) and 7 sterols (12–18) from the ethanol extract of E. dentata, identifying one new compound and 19 known compounds. Within spectroscopic methods such as NMR, HR-ESI-MS, and ECD, the structures and absolute configurations of these compounds were established. Among them, dentatacid A (11) possesses an unprecedented 2, 3-seco-arbor-2, 3-dioic skeleton within the potential biosynthetic pathway proposed. Dentatacid A also exhibited excellent anti-proliferative activity against the HT-29 (human colorectal adenocarcinoma) cell line, with an IC50 value of 2.64 ± 0.78 μM, which was further confirmed through network pharmacology and molecular docking. This study significantly expands the chemical diversity of E. dentata and offers new insights into the resource utilization and management of this invasive plant from the perspective of natural product discovery.
A Novel Method for the Enhancement of Sunflower Growth from Animal Bones and Chicken Feathers
Authors: Laila Ume , ul Huda Mishkat , Shakoor Isha , Nazir Aisha , Shafiq Muhammad , e Bareen Firdaus , Shaukat Kamran , Alam Talha Mahboob
Published: September 2024

The present study aimed at converting meat industry waste, particularly waste bones and chicken feathers, into biochar to recycle valuable nutrients present in it, which ultimately become part of the municipal waste. The bone biochar (BB) and feathers biochar (FB) were prepared at 550 °C, and their potential was evaluated as an organic amendment for the growth of sunflower. The ash content (AC) and fixed carbon (FC) improved significantly in prepared biochars as compared to raw feedstock. Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDX) analyses signaled the occurrence of various functional groups viz. amide group and hydroxyapatite, porosity, and multiple nutrients. Application of BB and FB in potted soil alone as well as in composites (1:1, 1:2, 2:1) at 1%, 3%, and 5% (w/w) and synthetic fertilizer significantly increased soil pH, electrical conductivity (ECe), organic matter (OM) and water holding capacity (WHC), while reducing the bulk density (BD). The growth of plants grown in soil treated with a 2:1 composite of feathers and bone biochar at 5% application rate showed significantly greater differences in plant height, total chlorophyll content, and plant dry weight than the control but was comparable to growth with chemical fertilizer, rendering it a potential alternative to chemical-based synthetic fertilizer.
Prediction Accuracy of Hyperelastic Material Models for Rubber Bumper under Compressive Load
Authors: Huri Dávid
Published: September 2024

Different hyperelastic material models (Mooney-Rivlin, Yeoh, Gent, Arruda-Boyce and Ogden) are able to estimate Treloar’s test data series containing uniaxial and biaxial tension and pure shear stress-strain characteristics of rubber. If the rubber behaviour is only determined for the specific load of the product, which, in the case of rubber bumpers, is the compression, the time needed for the laboratory test can be significantly decreased. The stress-strain characteristics of the uniaxial compression test of rubber samples were used to fit hyperelastic material models. Laboratory and numerical tests of a rubber bumper with a given compound and complex geometry were used to determine the accuracy of the material models. Designing rubber products requires special consideration of the numerical discretization process due to the nonlinear behaviours (material nonlinearity, large deformation, connections, etc.). Modelling considerations were presented for the finite element analysis of the rubber bumper. The results showed that if only uniaxial compression test data are available for the curve fitting of the material model, the Yeoh model performs the best in predicting the rubber product material response under compressive load and complex strain state.
From Waste to Styrene–Butadiene (SBR) Reuse: Developing PP/SBR/SEP Mixtures with Carbon Nanotubes for Antistatic Application
Authors: Sobrinho Edson Duarte de Melo , Ferreira Eduardo da Silva Barbosa , da Silva Flávio Urbano , Bezerra Elieber Barros , Wellen Renate Maria Ramos , Araújo Edcleide Maria , Luna Carlos Bruno Barreto
Published: September 2024

Styrene–butadiene rubber (SBR) waste from the shoe industry was repurposed to produce polypropylene (PP)-based compounds, with the aim of evaluating their antistatic potential. Styrene–ethylene–propylene (SEP) was added as a compatibilizing agent, while carbon nanotubes (MWCNT) were incorporated as a conductive nanofiller. The polymer compounds were processed in an internal mixer, and injection molded. The properties evaluated included torque rheometry, melt flow index (MFI), impact strength, tensile strength, Shore D hardness, electrical conductivity, heat deflection temperature (HDT), and differential scanning calorimetry (DSC), along with scanning electron microscopy (SEM) for morphology analysis. The production of the PP/SBR/SEP (60/30/10 wt%) compound resulted in a ductile material, enhancing impact strength and elongation at break to 161.2% and 165.2%, respectively, compared to pure PP. The addition of SEP improved the compatibility of the PP/SBR system, leading to an increase in the torque curve and a reduction in the MFI. Furthermore, the SBR/SEP combination in PP accelerated the crystallization process and increased the degree of crystallinity, suggesting a nucleating effect. Carbon nanotubes, in concentrations ranging from 0.5 to 2 phr (parts per hundred resin), were added to the PP/SBR/SEP system. Only the PP/SBR/SEP/MWCNT compound with 2 phr of MWCNT was suitable for antistatic applications, exhibiting an electrical conductivity of 4.52 × 10−07 S/cm. This was due to the greater distribution of MWCNT in the PP matrix, as demonstrated by SEM. In addition, remains tough at room temperature, with a 166% increase in impact strength compared to PP. However, there was a reduction in elastic modulus, tensile strength, Shore D hardness, and HDT due to increased flexibility. SBR waste can be reintegrated into the production chain to produce antistatic polymeric compounds, obtaining a tough material at room temperature.
Impact of Inverse Manganese Promotion on Silica-Supported Cobalt Catalysts for Long-Chain Hydrocarbons via Fischer–Tropsch Synthesis
Authors: Thibanyane Ntebogang , Gorimbo Joshua , Yao Yali
Published: September 2024

One of the challenges in Fischer–Tropsch synthesis (FTS) is the high reduction temperatures, which cause sintering and the formation of silicates. These lead to pore blockages and the coverage of active metals, particularly in conventional catalyst promotion. To address the challenge, this article investigates the effects of the preparation method, specifically the inverse promotion of SiO2-supported Co catalysts with manganese (Mn), and their reduction in H2 for FTS. The catalysts were prepared using stepwise incipient wetness impregnation of a cobalt nitrate precursor into a promoted silica support. The properties of the catalysts were characterized using XRD, XPS, TPR, and BET techniques. The structure–performance relationship of the inversely promoted catalysts in FTS was studied using a fixed-bed reactor to obtain the best performing catalysts for heavy hydrocarbons (C5+). XRD and XPS results indicated that Co3O4 is the dominant cobalt phase in oxidized catalysts. It was found that with increase in Mn loading, the reduction temperature increased in the following sequence 10%Co/SiO2 < 10%Co/0.25%Mn-SiO2 < 10%Co/0.5%Mn-SiO2 < 10%Co/3.0%Mn-SiO2. The catalyst with the lowest Mn loading, 10%Co/0.25%Mn-SiO2, exhibited higher C5+ selectivity, which can be attributed to less MSI and higher reducibility. This catalyst showed the lowest CH4 selectivity possibly due to lower H2 uptake and higher CO chemisorption.
Interpreting Visuality in the Middle Ages: The Iconographic Paradigm of the Refectory of the Monastery of San Salvador de Oña
Authors: Cuesta Sánchez Ana Maria
Published: September 2024

The research area of the interaction between religion, politics, and medieval Hispanic art has been pioneering and at the cutting edge of contemporary historiography in recent years. The deployment of iconographic and calligraphic motifs in medieval religious spaces has been identified as a key means of propagating legitimating messages of power and disseminating Christian doctrines. This interdisciplinary study proposes an analysis of the artistic vestiges present in the refectory of the Monastery of San Salvador de Oña (Burgos), with a view to establishing their decorative evolution during the 14th century and their symbolic significance. In order to establish a chronological proposal for the remains, the historical, artistic, and architectural contexts of the monastery are analyzed. This is complemented by a study of materials and techniques and a symbolic and iconographic analysis of the signs of power and evangelical concepts from the time of Pedro I until the transition to the Trastámara dynasty. In this way, a cross-disciplinary investigation is proposed, with the aim of elucidating how a sacred artistic work situated within a secular context can serve multiple purposes of political and religious legitimation through the adaptation of its iconography over time.
An Overlooked Jewish Community: The Jews of Rodoscuk in the 17th Century
Authors: Ekin Umit , Kerim Mehmet
Published: September 2024

This article addresses the intercommunal relations between the 17th century Jewish community of Rodoscuk and other social groups. It examines the community’s social structure, housing distribution and economic activities, placing particular emphasis on its interactions with Muslims in areas like trade, religious practices and social dynamics. By drawing on local court records, particularly the Qadi Registers, this article reveals how these interactions were shaped by the legal and social frameworks of their day, highlighting the complexities of coexistence in those contexts. In doing so, it evidences not only that Rodoscuk’s Jewish community took part in vibrant economic exchanges with its Muslim neighbors but also that it engaged with common social and religious contexts. Via a detailed analysis of these records, this study offers new insights into the nature of intercommunal relations in Rodoscuk. It challenges the often-assumed narrative of segregation and conflict in Ottoman society by testing existing claims in the literature through the case of the Jews of Rodoscuk. It concludes that Jews in this region in fact lived in relative harmony with Muslims and other groups in their vicinity.
Vibration Propulsion in Untethered Insect-Scale Robots with Piezoelectric Bimorphs and 3D-Printed Legs
Authors: Ramírez-Palma Mario Rodolfo , Ruiz-Díez Víctor , Corsino Víctor , Sánchez-Rojas José Luis
Published: September 2024

This research presents the development and evaluation of a miniature autonomous robot inspired by insect locomotion, capable of bidirectional movement. The robot incorporates two piezoelectric bimorph resonators, 3D-printed legs, an electronic power circuit, and a battery-operated microcontroller. Each piezoelectric motor features ceramic plates measuring 15 × 1.5 × 0.6 mm3 and weighing 0.1 g, with an optimized electrode layout. The bimorphs vibrate at two flexural modes with resonant frequencies of approximately 70 and 100 kHz. The strategic placement of the 3D-printed legs converts out-of-plane motion into effective forward or backward propulsion, depending on the vibration mode. A differential drive configuration, using the two parallel piezoelectric motors and calibrated excitation signals from the microcontroller, allows for arbitrary path navigation. The fully assembled robot measures 29 × 17 × 18 mm3 and weighs 7.4 g. The robot was tested on a glass surface, reaching a maximum speed of 70 mm/s and a rotational speed of up to 190 deg./s, with power consumption of 50 mW, a cost of transport of 10, and an estimated continuous operation time of approximately 6.7 h. The robot successfully followed pre-programmed paths, demonstrating its precise control and agility in navigating complex environments, marking a significant advancement in insect-scale autonomous robotics.