bluuebunny/crossref_metadata_2025 huggingface dataset:
Association Between Adjuvant Sorafenib and the Prognosis of Patients With Hepatocellular Carcinoma at a High Risk of Recurrence After Radical Resection
Authors: Li Qingli , Song Tianqiang
Published: September 2021

Background

The use of sorafenib in the adjuvant management of hepatocellular carcinoma (HCC) is controversial.

Aim

To analyze the effects of adjuvant sorafenib therapy in patients with HCC at high recurrence risk after radical resection.

Methods

This was a retrospective study of patients who underwent radical resection (R0 resection) for HCC at the Cancer Hospital of Tianjin Medical University between August 2009 and August 2017. All patients had microvascular invasion and were evaluated for portal vein tumor thrombus. The outcomes were overall survival (OS), recurrence-free survival (RFS), and survival after recurrence. Propensity score matching (PSM) was used.

Results

Before matching, there were 56 and 167 patients in the sorafenib and non-sorafenib groups. After PSM, there were 42 patients/group, and there were no significant differences in patient characteristics (all P>0.05). After PSM, compared with the non-sorafenib group, the sorafenib group showed longer median OS (34 vs. 26 months, P=0.032) and survival after recurrence (16 vs. 9 months, P=0.002), but no difference in RFS (14 vs. 11 months, P=0.564). Adjuvant sorafenib was the only factor independently associated with OS (HR=0.619, 95% CI: 0377–0.994, P=0.047). No factors were independently associated with RFS (all P>0.05).

Conclusion

Although adjuvant sorafenib therapy for patients with HCC and high recurrence risk does not reduce the recurrence risk of HCC, it might be associated with longer survival and a lower risk of death.
Prediction Models for Complete Resection in Secondary Cytoreductive Surgery of Patients With Recurrent Ovarian Cancer
Authors: Jiang Caixia , Li Zhengyu
Published: September 2021

The most advanced epithelial ovarian cancer develops recurrent disease despite maximal surgical cytoreduction and adjuvant platinum-based chemotherapy. Treatment with secondary cytoreductive surgery (SCS) combined with chemotherapy or with chemotherapy alone for patients with platinum-sensitive recurrent ovarian cancer (ROC) is currently under heated discussion. Encouragingly, the results of the AGO DESKTOP III Study and the SOC1/SGOG-OV2 trial, which have been published recently, showed a striking advantage in terms of overall survival (OS) and progression-free survival (PFS) of ROC patients undergoing SCS compared to chemotherapy alone; moreover, a benefit of SCS exclusively for patients with complete gross resection (CGR) was particularly highlighted. CGR is considered the ultimate goal of SCS, on condition that the balance between maximal survival gain and minimal operative morbidity is maintained. Several models have been proposed to predict the rate of CGR, such as the MSK criteria, the AGO score, and the Tian model, over the last 15 years. This summary is mainly about the several previously published prediction models for CGR in SCS of ROC patients and discusses the effectiveness and limitations of these prediction models.
Case Report: The Monogenic Familial Steroid-Resistant Nephrotic Syndrome Caused by a Novel Missense Mutation of NPHS2 Gene A593C in a Chinese Family
Authors: Bai Ling , Zhuang Jing , Zhang Changrong , Lu Chen , Tian Xuefei , Jiang Hong
Published: September 2021

Background: Pathogenic variants in the NPHS2 gene encoding podocin in kidney podocytes are associated with autosomal recessive steroid-resistant nephrotic syndrome (SRNS) by disrupting podocyte function and the integrity of the glomerular filtration barrier. The outcome is generally poor by progressing into end-stage kidney disease (ESKD). With the help of gene diagnostics, we can further understand the role of podocin of podocytes in the development and progression of SRNS. However, the pathological mutation of NPHS2 and clinical relevance remain further elusive.

Case Presentation: Two siblings, a 15-year-old girl and her 10-year-old younger brother from a consanguineous Chinese family, presented with nephrotic syndrome. Both of them developed progressive proteinuria starting from the 5-year-old of age. The renal pathological lesions for them revealed focal segmental glomerulosclerosis (FSGS). There was no response to the glucocorticoid, calcineurin inhibitors, and rituximab treatment. The female affected patient received the hemodialysis treatment due to ESKD in June 2020; the male patient was still in follow-up presenting with SRNS. The mutational screening of the two patients and their parents using Trio whole-exome sequencing showed the NPHS2 gene de novo missense mutation in exon 5 (A593C), for which the two siblings were homozygous and their parents confirmed heterozygous asymptomatic carriers. No other SRNS-related gene variants with the SRNS were determined.

Conclusion: Pathological gene variants screening in children clinically suspected with SRNS might be helpful in the diagnosis as well as appropriate decisions on treatment strategies and prediction of prognosis.
Mechanistic Coupling of a Novel in silico Cotyledon Perfusion Model and a Physiologically Based Pharmacokinetic Model to Predict Fetal Acetaminophen Pharmacokinetics at Delivery
Authors: Mian Paola , Nolan Bridget , van den Anker John N. , van Calsteren Kristel , Allegaert Karel , Lakhi Nisha , Dallmann André
Published: September 2021

Little is known about placental drug transfer and fetal pharmacokinetics despite increasing drug use in pregnant women. While physiologically based pharmacokinetic (PBPK) models can help in some cases to shed light on this knowledge gap, adequate parameterization of placental drug transfer remains challenging. A novel in silico model with seven compartments representing the ex vivo cotyledon perfusion assay was developed and used to describe placental transfer and fetal pharmacokinetics of acetaminophen. Unknown parameters were optimized using observed data. Thereafter, values of relevant model parameters were copied to a maternal-fetal PBPK model and acetaminophen pharmacokinetics were predicted at delivery after oral administration of 1,000 mg. Predictions in the umbilical vein were evaluated with data from two clinical studies. Simulations from the in silico cotyledon perfusion model indicated that acetaminophen accumulates in the trophoblasts; simulated steady state concentrations in the trophoblasts were 4.31-fold higher than those in the perfusate. The whole-body PBPK model predicted umbilical vein concentrations with a mean prediction error of 24.7%. Of the 62 concentration values reported in the clinical studies, 50 values (81%) were predicted within a 2-fold error range. In conclusion, this study presents a novel in silico cotyledon perfusion model that is structurally congruent with the placenta implemented in our maternal-fetal PBPK model. This allows transferring parameters from the former model into our PBPK model for mechanistically exploring whole-body pharmacokinetics and concentration-effect relationships in the placental tissue. Further studies should investigate acetaminophen accumulation and metabolism in the placenta as the former might potentially affect placental prostaglandin synthesis and subsequent fetal exposure.
Screening for Susceptibility-Related Biomarkers of Diclofenac-Induced Liver Injury in Rats Using Metabolomics
Authors: Tu Can , Gao Yuan , Song Di , Niu Ming , Ma Run-ran , Zhou Ming-xi , He Xian , Xiao Xiao-he , Wang Jia-bo
Published: September 2021

Early identification of individuals susceptible to idiosyncratic drug-induced liver injury (IDILI) is a challenging unmet demand. Diclofenac, one of the most widely available over-the-counter drugs for pain management worldwide, may induce liver dysfunction, acute liver failure, and death. Herein, we report that diclofenac-related hepatobiliary adverse reactions occurred more frequently in cases with immune activation. Furthermore, experiments with rats demonstrated divergent hepatotoxicity responses in individuals exposed to diclofenac, and modest inflammation potentiated diclofenac-induced liver injury. Susceptible rats had unique plasma metabolomic characteristics, and as such, the metabolomic approach could be used to distinguish susceptible individuals. The 23 identified susceptibility-related metabolites were enriched by several metabolic pathways related to acute-phase reactions of immunocytes and inflammatory responses, including sphingolipid, tyrosine, phenylalanine, tryptophan, and lipid metabolism pathways. This finding implies a mechanistic role of metabolic and immune disturbances affects susceptibility to diclofenac-IDILI. Further nine metabolite biomarkers with potent diagnostic capabilities were identified using receiver operating characteristic curves. These findings elucidated the potential utility of metabolomic biomarkers to identify individuals susceptible to drug hepatotoxicity and the underlying mechanism of metabolic and immune disturbances occurring in IDILI.
Development of Genome-Wide Functional Markers Using Draft Genome Assembly of Guava (Psidium guajava L.) cv. Allahabad Safeda to Expedite Molecular Breeding
Authors: Thakur Sujata , Yadav Inderjit Singh , Jindal Manish , Sharma Parva Kumar , Dhillon Guriqbal Singh , Boora Rajbir Singh , Arora Naresh Kumar , Gill Manav Indra Singh , Chhuneja Parveen , Mittal Amandeep
Published: September 2021

Guava (Psidium guajava L.), a rich source of nutrients, is an important tropical and subtropical fruit of the Myrtaceae family and exhibits magnificent diversity. Genetic diversity analysis is the first step toward the identification of parents for hybridization, genetic mapping, and molecular breeding in any crop species. A diversity analysis based on whole-genome functional markers increases the chances of identifying genetic associations with agronomically important traits. Therefore, here, we sequenced the genome of guava cv. Allahabad Safeda on an Illumina platform and generated a draft assembly of ~304 MB. The assembly of the Allahabad Safeda genome constituted >37.95% repeat sequences, gene prediction with RNA-seq data as evidence identified 14,115 genes, and BLAST n/r, Interproscan, PfamScan, BLAST2GO, and KEGG annotated 13,957 genes. A comparative protein transcript analysis of tree species revealed the close relatedness of guava with Eucalyptus. Comparative transcriptomics-based SSR/InDel/SNP-PCR ready genome-wide markers in greenish-yellow skinned and white fleshed-Allahabad Safeda to four contrasting cultivars viz apple-color-skinned and white-fleshed-Lalima, greenish-yellow-skinned and pink-fleshed-Punjab Pink, purple-black-skinned and purple-fleshed-Purple Local and widely used rootstock-Lucknow-49 were developed. The molecular markers developed here revealed a high level of individual heterozygosity within genotypes in 22 phenotypically diverse guava cultivars. Principal coordinate, STRUCTURE clustering, and neighbor-joining-based genetic diversity analysis identified distinct clusters associated with fruit skin and flesh color. The genome sequencing of guava, functional annotation, comparative transcriptomics-based genome-wide markers, and genetic diversity analysis will expand the knowledge of genomes of climacteric fruits, facilitating trait-based molecular breeding and diversifying the nutritional basket.
Transcriptome Reprogramming of Tomato Orchestrate the Hormone Signaling Network of Systemic Resistance Induced by Chaetomium globosum
Authors: Singh Jagmohan , Aggarwal Rashmi , Bashyal Bishnu Maya , Darshan K. , Parmar Pooja , Saharan M. S. , Hussain Zakir , Solanke Amolkumar U.
Published: September 2021

Chaetomium globosum is a potential biological control agent effective against various plant pathogens. Several reports are available on the mycoparastism and antibiosis mechanisms of C. globosum against plant pathogenic fungi, whereas a few states induced resistance. The potential induced defense component of C. globosum (Cg-2) was evaluated against early blight disease of tomato (Solanum lycopersicum) and further, global RNA sequencing was performed to gain deep insight into its mechanism. The expression of marker genes of hormone signaling pathways, such as PR1, PiII, PS, PAL, Le4, and GluB were analyzed using real-time quantitative reverse transcription PCR (qRT-PCR) to determine the best time point for RNA sequencing. The transcriptome data revealed that 22,473 differentially expressed genes (DEGs) were expressed in tomato at 12 h post Cg-2 inoculation as compared with control plants and among these 922 DEGs had a fold change of −2 to +2 with p < 0.05. The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed that most of the DEGs were belonging to metabolic pathways, biosynthesis of secondary metabolites, plant–pathogen interaction, chlorophyll metabolism, and plant hormone signal transduction. Gene Ontology (GO) analysis revealed that DEGs were enriched mainly related to binding activity (GO:0005488), catalytic activity (GO:0003824), metabolic process (GO:0008152), cellular process (GO:0009987), response to stimulus (GO:0050896), biological regulation (GO:0065007), and transcription regulator activity (GO:0140110). The gene modulations in hormone signaling transduction, phenylpropanoid biosynthesis, and mitogen-activated protein kinases (MPK) signaling indicated the upregulation of genes in these pathways. The results revealed active participation of jasmonic acid (JA) and salicylic acid (SA) signaling transduction pathways which further indicated the involvement of induced systemic resistance (ISR) and systemic acquired resistance (SAR) in the systemic resistance induced by Cg-2 in tomato.
A Novel Banana Mutant “RF 1” (Musa spp. ABB, Pisang Awak Subgroup) for Improved Agronomic Traits and Enhanced Cold Tolerance and Disease Resistance
Authors: Wang Xiaoyi , Wang Anbang , Li Yujia , Xu Yi , Wei Qing , Wang Jiashui , Lin Fei , Gong Deyong , Liu Fei , Wang Yanting , Peng Liangcai , Li Jingyang
Published: September 2021

Banana is a major fruit crop grown in tropical and subtropical regions worldwide. Among cultivars, “FenJiao, FJ” (Musa spp. ABB, Pisang Awak subgroup) is a popular variety of bananas, due to its better sugar-acid blend and relatively small fruit shape. However, because the traditional FJ variety grows relatively high in height, it is vulnerable to lodging and unsuitable for harvesting. In this study, we sought desirable banana mutants by carrying out ethyl methanesulfonate (EMS) mutagenesis with the FJ cultivar. After the FJ shoot tips had been treated with 0.8% (v/v) EMS for 4 h, we obtained a stably inherited mutant, here called “ReFen 1” (RF1), and also observed a semi-dwarfing phenotype. Compared with the wild type (FJ), this RF1 mutant featured consistently improved agronomic traits during 5-year field experiments conducted in three distinct locations in China. Notably, the RF1 plants showed significantly enhanced cold tolerance and Sigatoka disease resistance, mainly due to a substantially increased soluble content of sugar and greater starch accumulation along with reduced cellulose deposition. Therefore, this study not only demonstrated how a powerful genetic strategy can be used in fruit crop breeding but also provided insight into the identification of novel genes for agronomic trait improvement in bananas and beyond.
The Similarities and Distances of Growth Rates Related to COVID-19 Between Different Countries Based on Spectral Analysis
Authors: Chen Ray-Ming
Published: September 2021

The COVID-19 pandemic has taken more than 1.78 million of lives across the globe. Identifying the underlying evolutive patterns between different countries would help us single out the mutated paths and behavior of this virus. I devise an orthonormal basis which would serve as the features to relate the evolution of one country's cases and deaths to others another's via coefficients from the inner product. Then I rank the coefficients measured by the inner product via the featured frequencies. The distances between these ranked vectors are evaluated by Manhattan metric. Afterwards, I associate each country with its nearest neighbor which shares the evolutive pattern via the distance matrix. Our research shows such patterns is are not random at all, i.e., the underlying pattern could be contributed to by some factors. In the end, I perform the typical cosine similarity on the time-series data. The comparison shows our mechanism differs from the typical one, but is also related to each it in some way. These findings reveal the underlying interaction between countries with respect to cases and deaths of COVID-19.
Association of microRNAs With Embryo Development and Fertilization in Women Undergoing Subfertility Treatments: A Pilot Study
Authors: Butler Alexandra E. , Cunningham Thomas Keith , Ramachandran Vimal , Diboun Ilhame , Halama Anna , Sathyapalan Thozhukat , Najafi-Shoushtari S. Hani , Atkin Stephen L.
Published: September 2021

Objective: Small non-coding RNAs, known as microRNAs (miRNAs), have emerging regulatory functions within the ovary that have been related to fertility. This study was undertaken to determine if circulating miRNAs reflect the changes associated with the parameters of embryo development and fertilization.

Methods: In this cross-sectional pilot study. Plasma miRNAs were collected from 48 sequentially presenting women in the follicular phase prior to commencing in vitro fertilization (IVF). Circulating miRNAs were measured using locked nucleic acid (LNA)-based quantitative PCR (qPCR), while an updated miRNA data set was used to determine their level of expression.

Results: Body mass index and weight were associated with the miRNAs let7b-3p and miR-375, respectively (p < 0.05), with the same relationship being found between endometrium thickness at oocyte retrieval and miR-885-5p and miR-34a-5p (p < 0.05). In contrast, miR-1260a was found to be inversely associated with anti-Mullerian hormone (AMH; p = 0.007), while miR-365a-3p, miR122-5p, and miR-34a-5p correlated with embryo fertilization rates (p < 0.05). However, when omitting cases of male infertility (n = 15), miR122-5p remained significant (p < 0.05), while miR-365a-3p and miR-34a-5p no longer differed; interestingly, however, miR1260a and mir93.3p became significant (p = 0.0087/0.02, respectively). Furthermore, age was negatively associated with miR-335-3p, miR-28-5p, miR-155-5p, miR-501-3p, and miR-497-5p (p < 0.05). Live birth rate was negatively associated with miR-335-3p, miR-100-5p, miR-497-5p, let-7d, and miR-574-3p (p < 0.05), but these were not significant when age was accounted for.However, with the exclusion of male factor infertility, all those miRNAs were no longer significant, though miR.150.5p emerged as significant (p = 0.042). A beta-regression model identified miR-1260a, miR-486-5p, and miR-132-3p (p < 0.03, p = 0.0003, p < 0.00001, respectively) as the most predictive for fertilization rate. Notably, changes in detectable miRNAs were not linked to cleavage rate, top quality embryos (G3D3), and blastocyst or antral follicle count. An ingenuity pathway analysis showed that miRNAs associated with age were also associated with the variables found in reproductive system diseases.

Conclusion: Plasma miRNAs prior to the IVF cycle were associated with differing demographic and IVF parameters, including age, and may be predictive biomarkers of fertilization rate.
Impact of Storm-Enhanced Density (SED) on Ion Upflow Fluxes During Geomagnetic Storm
Authors: Zou Shasha , Ren Jiaen , Wang Zihan , Sun Hu , Chen Yang
Published: September 2021

The impact of the dynamic evolution of the Storm-Enhanced Density (SED) on the upward ion fluxes during the March 06, 2016 geomagnetic storm is studied using comprehensive multi-scale datasets. This storm was powered by a Corotating Interaction Region (CIR), and the minimum Sym-H reached ∼−110 nT. During the ionospheric positive storm phase, the SED formed and the associated plume and polar cap patches occasionally drifted anti-sunward across the polar cap. When these high-density structures encountered positive vertical flows, large ion upward fluxes were produced, with the largest upward flux reaching 3 × 1014 m−2s−1. These upflows were either the type-1 ion upflow associated with fast flow channels, such as the subauroral polarization stream (SAPS) channel, or the type-2 ion upflow due to soft particle precipitations in the cusp region. The total SED-associated upflow flux in the dayside cusp can be comparable to the total upflow flux in the nightside auroral zone despite the much smaller cusp area compared with the auroral zone. During the ionospheric negative storm phase, the ionospheric densities within the SED and plume decreased significantly and thus led to largely reduced upward fluxes. This event analysis demonstrates the critical role of the ionospheric high-density structures in creating large ion upward fluxes. It also suggests that the dynamic processes in the coupled ionosphere-thermosphere system and the resulting state of the ionospheric storm are crucial for understanding the temporal and spatial variations of ion upflow fluxes and thus should be incorporated into coupled geospace models for improving our holistic understanding of the role of ionospheric plasma in the geospace system.
A Survey of Priority Livestock Diseases and Laboratory Diagnostic Needs of Animal Health Professionals and Farmers in Uganda
Authors: Vudriko Patrick , Ekiri Abel B. , Endacott Isabella , Williams Sitira , Gityamwi Nyangi , Byaruhanga Joseph , Alafiatayo Ruth , Mijten Erik , Tweyongyere Robert , Varga Gabriel , Cook Alasdair J. C.
Published: September 2021

Background: Despite the investments made in veterinary diagnostic laboratory service delivery in Uganda, the scope and level of utilization remains low. This study aimed to determine the priority livestock diseases for which farmers and animal health professionals require veterinary diagnostic laboratory services, document the perceptions and opinions of key stakeholders on veterinary diagnostic laboratory services, and determine the factors that influence the delivery and utilization of animal disease diagnostic services in Uganda.

Methods: A qualitative study approach involving a survey and key informant interviews was used to collect relevant data from four stakeholder groups: animal health workers, laboratory technologists and technicians, farmers, and key informants. The survey data were exported to excel, and descriptive statistics performed. The key informant interview recordings were transcribed, and thematic analysis performed.

Results: The most reported diseases and conditions for which diagnostic services were needed were hemoparasites (including East Coast fever, anaplasmosis, babesiosis, and trypanosomosis), viral (including Foot and mouth disease, lumpy skin disease, rift valley fever, and papillomatosis), bacteria (including brucellosis, colibacillosis, anthrax, leptospirosis, and paratuberculosis) and protozoa diseases (coccidiosis), endoparasites (helminths), and mastitis. The most common diagnostic laboratory tests requested by clients, but laboratories were unable to provide included: rapid tests for contagious bovine pleuropneumonia, Foot and mouth disease, Newcastle disease, acaricide analysis, culture and antimicrobial sensitivity test, serology, and complete blood count. The most frequently reported challenges to providing diagnostic laboratory services were poor or lack of relevant equipment, insufficient or lack of supplies and reagents, high cost of reagents, inadequate or lack of laboratory staff to perform tests, and inadequate training of laboratory staff.

Conclusions: This study highlighted the need to improve provision of laboratory diagnostic services to meet the prioritized diagnostic needs of farmers and animal health professionals. Increased intersectoral engagement and funding support from the private, industry, and government sectors is necessary to help address the observed challenges to provision of diagnostic laboratory services, including equipping of the laboratories, provision of supplies, and hiring and training of laboratory staff. Finally, the findings also suggest that the education of farmers and animal health workers on the value and benefits of laboratory diagnostic services may contribute to increase in sample submission and subsequent demand for diagnostic laboratory services.
Animal Welfare Control—Inspection Findings and the Threshold for Requesting a Police Investigation
Authors: Valtonen Elli , Koskela Tarja , Valros Anna , Hänninen Laura
Published: September 2021

An increasing proportion of animal welfare violations in Finland are related to companion animals. However, only a small number of these issues are investigated or prosecuted. The aims of this study were (i) to describe the inspection findings and the resulting actions of the official municipal veterinarians in the Finnish Capital Region and (ii) to identify the factors that predict their submissions of investigation requests to the police. Our data consisted of animal welfare complaints and official veterinarians' inspection reports and decisions from 811 cases of animal welfare control in the Finnish Capital Region. The data covered the period from March 2019 to April 2020. We performed logistic regression analyses to identify the factors that best predict when official veterinarians detect non-compliances and report the cases for police investigation. In 86% (696/811) of the cases, the veterinarians performed at least one animal welfare inspection, and/or received information from the police, or otherwise investigated the complaint. The most common forms of non-compliance were lack of basic maintenance and care (42%, 295/696) and insufficient veterinary care (27%, 185/696). The veterinarians requested for a police investigation in 9.6% (44/460) of all cases with detected non-compliances. The best predictors for detecting non-compliances with the animal welfare legislation were complaints of insufficient veterinary care (OR 1.9, CI 1.1–3.4), the cases assessed by the information from the police and/or an animal shelter (OR 15.2, CI 7.9–29.2), at least one inspection in an animal's premises with prior warning (OR 11.2, CI 5.5–22.6), and without prior warning (OR 17.0, CI 9.7–29.5). Complaints of violence against animals were negatively associated with detecting non-compliances (OR 0.5, CI 0.3–0.9). However, the detection of violence against animals predicted requests for police investigations (OR 9.3, CI 3.1–27.9), as did the execution of permanent urgent measures by official veterinarians (OR 4.9, CI 1.9–12.9). To improve the animal welfare control system and the investigation of crimes against animals, cooperation between officials should be developed. Further studies are needed to improve the understanding of the prevalence of violence against animals, and to advance methods used by animal welfare control to identify cases of violence.
Halotolerant-Koccuria rhizophila (14asp)-Induced Amendment of Salt Stress in Pea Plants by Limiting Na+ Uptake and Elevating Production of Antioxidants
Authors: Khan Amir , Wang Tongtong , Hussain Tayyaba , Amna  , Ali Fayaz , Shi Fuchen , Latef Arafat , Ali Omar , Hayat Kashif , Mehmood Shehzad , Zainab Nida , Muneer Muhammad , Munis Muhammad , Soliman Mona , Chaudhary Hassan
Published: September 2021

Endophytic bacteria are useful for their safe services in plant growth improvement and for ameliorating abiotic and biotic stresses. Salt-tolerant plant-growth-promoting Kocuria rhizophila 14asp (accession number KF 875448) was investigated for its role in pea plants under a saline environment. Salt stress (75 mM and 150 mM NaCl) was subjected to two pea varieties, peas2009 and 9800-10, in a greenhouse under a complete randomized design. Different parameters such as plant growth promotion, relative water content, chlorophyll, antioxidants, and mineral contents were analyzed to elucidate the extent of tolerance persuaded by PGPB (plant-growth-promoting bacteria). Exhibition of adverse effects was noticed in uninoculated varieties. However, inoculation of K. rhizophila improved the morphological parameters, antioxidant enzymes, and minimized the uptake of Na+ in plants under various saline regimes. Pea variety 9800-10 exhibited more tolerance than peas2009 in all traits, such as root and shoot length, fresh and dry biomass, chlorophyll contents, and antioxidant enzymes. Our results showed that halotolerant K. rhizophila inoculation plays a vital role in enhancing plant growth by interacting ingeniously with plants through antioxidant systems, enduring saline conditions.
Reducing Dog Relinquishment to Rescue Centres Due to Behaviour Problems: Identifying Cases to Target with an Advice Intervention at the Point of Relinquishment Request
Authors: Powdrill-Wells Natalie , Taylor Sienna , Melfi Vicky
Published: September 2021

Behaviour problems are a leading reason for dogs being relinquished to rescue centres across the world every year. The aim of this study was to investigate whether free behavioural advice would be accepted at the point of an owner requesting to relinquish their dog for behavioural reasons. The call records of 1131 relinquishment requests were reviewed and analysed to establish if the offer of free behaviour advice was accepted. The results showed that advice was accepted in 24.4% of relinquishment requests and behavioural problem was a significant predictor of whether advice was accepted (p < 0.001). The odds of advice being accepted were 5.755 times (95% CI: 2.835–11.681; p < 0.001) greater for a relinquishment request due to problems with general management behaviours compared to aggression between dogs in the home, representing 4.2% and 20.2% of overall relinquishment requests. These data suggest that owners are prepared to accept behaviour advice at the point of relinquishment request, so advice interventions could have potential to impact the levels of dog relinquishment to rescue centres. The impact of an intervention offering behaviour advice may be limited by overall levels of advice acceptance by owners and therefore complimentary proactive solutions to reduce behavioural relinquishments should also be considered.
Analysis of mRNA and Long Non-Coding RNA Expression Profiles in Developing Yorkshire Pig Spleens
Authors: Li Xinjian , Han Xuelei , Sun Caixia , Li Gaiying , Wang Kejun , Li Xiuling , Qiao Ruimin
Published: September 2021

Epidemic diseases cause great economic loss in pig farms each year; some of these diseases are characterized mainly in the spleen, but mRNA and lncRNA (long non-coding RNA) expression networks in developing Yorkshire pig spleens remain obscure. Here, we profiled the systematic characters of mRNA and lncRNA repertoires in three groups of spleens from nine Yorkshire pigs, each three aged at seven days, 90 days, and 180 days. By using a precise mRNA and lncRNA identification pipeline, we identified 19,647 genes and 219 known and 3219 putative lncRNA transcripts; 1729 genes and 64 lncRNAs therein were found to express differentially. The gene expression characteristics of genes and lncRNAs were found to be basically fixed before 90 days after birth. Three large gene expression modules were detected. The enrichment analyses of differentially expressed genes and the potential target genes of differentially expressed lncRNAs both displayed the crucial roles of up-regulation in immune activation and hematopoiesis, and down-regulation in cell replication and division in 90 days and 180 days compared to seven days. ENSSSCT00000001325 was the only lncRNA transcript that existed in the three groups. CDK1, PCNA, and PLK were detected to be node genes that varied with age. This study contributes to a further understanding of mRNA and lncRNA expression in different developmental pig spleens.
A Disposable Electrochemical Biosensor Based on Screen-Printed Carbon Electrodes Modified with Silver Nanowires/HPMC/Chitosan/Urease for the Detection of Mercury (II) in Water
Authors: Saenchoopa Apichart , Klangphukhiew Supannika , Somsub Rachata , Talodthaisong Chanon , Patramanon Rina , Daduang Jureerut , Daduang Sakda , Kulchat Sirinan
Published: September 2021

This work describes the facile preparation of a disposable electrochemical biosensor for the detection of Hg(II) in water by modifying the surface of a screen-printed carbon electrode (SPCE). The surface modification consists of the immobilization of a composite layer of silver nanowires, hydroxymethyl propyl cellulose, chitosan, and urease (AgNWs/HPMC/CS/Urease). The presence of the composite was confirmed by scanning electron microscopy (SEM) and its excellent conductivity, due chiefly to the electrical properties of silver nanowires, enhanced the sensitivity of the biosensor. Under optimum conditions, the modified SPCE biosensor showed excellent performance for the detection of Hg(II) ions, with an incubation time of 10 min and a linear sensitivity range of 5–25 µM. The limit of detection (LOD) and limit of quantitation (LOQ) were observed to be 3.94 µM and 6.50 µM, respectively. In addition, the disposable and portable biosensor exhibited excellent recoveries for the detection of Hg(II) ions in commercial drinking water samples (101.62–105.26%). The results are correlated with those obtained from inductively coupled plasma optical emission spectrometry (ICP-OES), indicating that our developed sensor is a reliable method for detection of Hg(II) in real water samples. The developed sensor device is a simple, effective, portable, low cost, and user-friendly platform for real-time detection of heavy metal ions in field measurements with potential for other biomedical applications in the future.
The Genetics of Sleep Disorders in Children: A Narrative Review
Authors: Mainieri Greta , Montini Angelica , Nicotera Antonio , Di Rosa Gabriella , Provini Federica , Loddo Giuseppe
Published: September 2021

Sleep is a universal, highly preserved process, essential for human and animal life, whose complete functions are yet to be unravelled. Familial recurrence is acknowledged for some sleep disorders, but definite data are lacking for many of them. Genetic studies on sleep disorders have progressed from twin and family studies to candidate gene approaches to culminate in genome-wide association studies (GWAS). Several works disclosed that sleep-wake characteristics, in addition to electroencephalographic (EEG) sleep patterns, have a certain degree of heritability. Notwithstanding, it is rare for sleep disorders to be attributed to single gene defects because of the complexity of the brain network/pathways involved. Besides, the advancing insights in epigenetic gene-environment interactions add further complexity to understanding the genetic control of sleep and its disorders. This narrative review explores the current genetic knowledge in sleep disorders in children, following the International Classification of Sleep Disorders—Third Edition (ICSD-3) categorisation.
Three Needs and Information Anxiety on Knowledge Purchase Intentions across Online Knowledge Platforms
Authors: Lin Shinyi , Cheng Kohang , Chuang Shu-Hui
Published: September 2021

Given the recent advances in technology, knowledge-based products have become increasingly prevalent. Many companies offer interdisciplinary resources for incumbent learners to break through chronological and geographical constraints. Therefore, it is important to investigate the factors that motivate learners to pay for knowledge-based products. The purpose of this research was to identify the relevant factors that contribute to purchasing intentions and to clarify the reasons why people purchase knowledge-based products. This study involved 406 valid participants over 20 years of age with knowledge purchase experience. The results demonstrated that incumbent learners’ need for affiliation has a positive effect on involvement, and that involvement has a significant positive impact on knowledge purchase intentions. The key factor influencing learners to pay for knowledge-based products is their involvement in learning. Information anxiety interferes with the relationship between involvement and knowledge purchase intentions. However, no linear relationship was found between cognitive styles and involvement. Field-dependent learners show greater involvement and also information anxiety than field-independent learners. The research offers suggestions for practical use and future research from the perspective of knowledge-product marketing.
Overcoming Immunotherapy Resistance by Targeting the Tumor-Intrinsic NLRP3-HSP70 Signaling Axis
Authors: Theivanthiran Balamayooran , Haykal Tarek , Cao Linda , Holtzhausen Alisha , Plebanek Michael , DeVito Nicholas C. , Hanks Brent A.
Published: September 2021

The tumor-intrinsic NOD-like receptor family, pyrin-domain-containing-3 (NLRP3) inflammasome, plays an important role in regulating immunosuppressive myeloid cell populations in the tumor microenvironment (TME). While prior studies have described the activation of this inflammasome in driving pro-tumorigenic mechanisms, emerging data is now revealing the tumor NLRP3 inflammasome and the downstream release of heat shock protein-70 (HSP70) to regulate anti-tumor immunity and contribute to the development of adaptive resistance to anti-PD-1 immunotherapy. Genetic alterations that influence the activity of the NLRP3 signaling axis are likely to impact T cell-mediated tumor cell killing and may indicate which tumors rely on this pathway for immune escape. These studies suggest that the NLRP3 inflammasome and its secreted product, HSP70, represent promising pharmacologic targets for manipulating innate immune cell populations in the TME while enhancing responses to anti-PD-1 immunotherapy. Additional studies are needed to better understand tumor-specific regulatory mechanisms of NLRP3 to enable the development of tumor-selective pharmacologic strategies capable of augmenting responses to checkpoint inhibitor immunotherapy while minimizing unwanted off-target effects. The execution of upcoming clinical trials investigating this strategy to overcome anti-PD-1 resistance promises to provide novel insight into the role of this pathway in immuno-oncology.
Neurobiological Basis of Increased Risk for Suicidal Behaviour
Authors: Wisłowska-Stanek Aleksandra , Kołosowska Karolina , Maciejak Piotr
Published: September 2021

According to the World Health Organization (WHO), more than 700,000 people die per year due to suicide. Suicide risk factors include a previous suicide attempt and psychiatric disorders. The highest mortality rate in suicide worldwide is due to depression. Current evidence suggests that suicide etiopathogenesis is associated with neuroinflammation that activates the kynurenine pathway and causes subsequent serotonin depletion and stimulation of glutamate neurotransmission. These changes are accompanied by decreased BDNF (brain-derived neurotrophic factor) levels in the brain, which is often linked to impaired neuroplasticity and cognitive deficits. Most suicidal patients have a hyperactive hypothalamus–pituitary–adrenal (HPA) axis. Epigenetic mechanisms control the above-mentioned neurobiological changes associated with suicidal behaviour. Suicide risk could be attenuated by appropriate psychological treatment, electroconvulsive treatment, and drugs: lithium, ketamine, esketamine, clozapine. In this review, we present the etiopathogenesis of suicide behaviour and explore the mechanisms of action of anti-suicidal treatments, pinpointing similarities among them.
Characteristics of Clinical Trial Participants with Duchenne Muscular Dystrophy: Data from the Muscular Dystrophy Surveillance, Tracking, and Research Network (MD STARnet)
Authors: Mathews Katherine D. , Conway Kristin M. , Gedlinske Amber M. , Johnson Nicholas , Street Natalie , Butterfield Russell J. , Hung Man , Ciafaloni Emma , Romitti Paul A.
Published: September 2021

Background: Therapeutic trials are critical to improving outcomes for individuals diagnosed with Duchenne muscular dystrophy (DMD). Understanding predictors of clinical trial participation could maximize enrollment. Methods: Data from six sites (Colorado, Iowa, Piedmont region North Carolina, South Carolina, Utah, and western New York) of the Muscular Dystrophy Surveillance, Tracking, and Research Network (MD STARnet) were analyzed. Clinical trial participation and individual-level clinical and sociodemographic characteristics were obtained from medical records for the 2000–2015 calendar years. County-level characteristics were determined from linkage of the most recent county of residence identified from medical records and publicly available federal datasets. Fisher’s exact and Wilcoxon two-sample tests were used with statistical significance set at one-sided p-value (<0.05) based on the hypothesis that nonparticipants had fewer resources. Results: Clinical trial participation was identified among 17.9% (MD STARnet site: 3.7–27.3%) of 358 individuals with DMD. Corticosteroids, tadalafil, and ataluren (PTC124) were the most common trial medications recorded. Fewer non-Hispanic blacks or Hispanics than non-Hispanic whites participated in clinical trials. Trial participants tended to reside in counties with lower percentages of non-Hispanic blacks. Conclusion: Understanding characteristics associated with clinical trial participation is critical for identifying participation barriers and generalizability of trial results. MD STARnet is uniquely able to track clinical trial participation through surveillance and describe patterns of participation.
Novel Compound Heterozygous Variants of the ABCC8 Gene Warrant Identification of Pancreatic Histology in Infant with Diazoxide-unresponsive Congenital Hyperinsulinism
Authors: Al Balwi Rana , Bubshait Dalal , Al Nefily Raed , Al Ghamdi Omar
Published: September 2021

Congenital hyperinsulinism (CHI) is characterized by dysregulated insulin secretion, resulting in severe hypoglycemia. Mutations in the ABCC8 and KCNJ11 genes encoding KATP channels in beta cells of the pancreas are common among patients with CHI. Autosomal recessive CHI with diffuse involvement is the most common type of CHI among Saudi patients. It is relatively common for patients with autosomal recessive CHI to be medically unresponsive and undergo pancreatectomy. In this case report, we describe novel compound heterozygous variants in the ABCC8 gene in a Saudi infant that caused diazoxide-unresponsive CHI. The variants included a monoallelic paternally inherited variant that has been previously reported to cause a focal form of CHI and a maternally inherited variant of unknown significance (VUS). The severity of CHI in this patient was mild over the one-year follow-up period, with a near-optimal glycemic response on a low dose of octreotide. We suspected an atypical subtype of histological involvement in the patient. In this report, we highlight the phenotypic spectrum of novel compound heterozygous variants in a patient with CHI and consider that the report can help establish the pathogenicity of the VUS.
Fabrication of a 3.5-GHz Solidly Mounted Resonator by Using an AlScN Piezoelectric Thin Film
Authors: Chung Chan-Yu , Chen Ying-Chung , Chen Yu-Cheng , Kao Kuo-Sheng , Chang Yu-Chen
Published: September 2021

In this study, a 3.5-GHz solidly mounted resonator (SMR) was developed by doping scandium in aluminum nitride to form AlScN as the piezoelectric thin film. Molybdenum (Mo) of 449 nm thickness and silicon dioxide (SiO2) of 371 nm thickness were used as the high and low acoustic impedance films, respectively, which were alternately stacked on a silicon substrate to form a Bragg reflector. Then, an alloy target with atomic ratio of 15% Sc was adopted to deposit the piezoelectric AlScN thin film on the Bragg reflector, using a radio frequency magnetron sputtering system. The characteristics of the c-axis orientation of the AlScN thin films were optimized by adjusting sputtering parameters as sputtering power of 250 W, sputtering pressure of 20 mTorr, nitrogen gas ratio of 20%, and substrate temperature of 300 °C. Finally, a metal top electrode was coated to form a resonator. The X-ray diffraction (XRD) analysis showed that the diffraction peak angles of the AlScN film shifted towards lower angles in each crystal phase, compared to those of AlN film. The energy dispersive X-ray spectrometer (EDX) analysis showed that the percentage of scandium atom in the film is about 4.5%, regardless of the sputtering conditions. The fabricated resonator exhibited a resonance frequency of 3.46 GHz, which was a small deviation from the preset resonance frequency of 3.5 GHz. The insertion loss of −10.92 dB and the electromechanical coupling coefficient of 2.24% were obtained. As compared to the AlN-based device, the AlScN-based resonator exhibited an improved electromechanical coupling coefficient by about two times.
Patterns of Leaf Morphological Traits of Beech (Fagus sylvatica L.) along an Altitudinal Gradient
Authors: Adamidis George C. , Varsamis Georgios , Tsiripidis Ioannis , Dimitrakopoulos Panayiotis G. , Papageorgiou Aristotelis C.
Published: September 2021

Broadleaved tree species in mountainous populations usually demonstrate high levels of diversity in leaf morphology among individuals, as a response to a variety of environmental conditions associated with changes in altitude. We investigated the parameters shaping leaf morphological diversity in 80 beech individuals (Fagus sylvatica L.), in light and shade leaves, growing along an elevational gradient and under different habitat types on Mt. Paggeo in northeastern Greece. A clear altitudinal pattern was observed in the morphological leaf traits expressing lamina size and shape; with increasing altitude, trees had leaves with smaller laminas, less elongated outlines, and fewer pairs of secondary veins. However, this altitudinal trend in leaf morphology was varied in different habitat types. Furthermore, the shade leaves and light leaves showed differences in their altitudinal trend. Traits expressing lamina shape in shade leaves were more related to altitude, while leaf size appeared to be more influenced by habitat type. While the altitudinal trend in leaf morphology has been well documented for numerous broadleaved tree species, in a small spatial scale, different patterns emerged across different habitat types. This morphological variability among trees growing in a mountainous population indicates a high potential for adaptation to environmental extremes.
Impacts of COVID-19 on the Green Industry
Authors: Marwah Pulkit , Zhang Yu Yvette , Gu Mengmeng
Published: September 2021

The COVID-19 pandemic has forced many horticultural businesses to alter the way they operate. This includes, but may not be limited to, changing hours of operation, working with limited staff, and restricting customer access indoors. This could result in several challenges for businesses. In this study, we evaluate the impacts of COVID-19 on the green industry in the U.S. and identify the challenges for businesses. Based on our research findings, the major challenges faced by businesses were not having enough employees and inventory to keep up with consumer demand during COVID-19. We also evaluate the effect of the pandemic on the sales of different plants, gardening products/services, and the overall revenues of businesses. For different types of plants included in the survey, landscape herbaceous flowers, landscape shrubs, and landscape trees showed the most significant increase in sales. In addition, for different gardening products/services, container plants, small plants, and soil and compost showed the most significant increase in sales. A total of 64% of the businesses reported higher overall sales compared to the same season’s previous year, while less than 24% of the businesses reported decreased sales. Moreover, over 46% of the businesses indicated that COVID-19 had positively affected their sales in 2020, while 31% thought that it had negatively impacted their sales. These findings imply a greater demand potential for plants and gardening products/services from consumers, during this pandemic.