bluuebunny/crossref_metadata_2025 huggingface dataset:
High-Level Rovibrational Calculations on Ketenimine
Authors: Tschöpe Martin , Schröder Benjamin , Erfort Sebastian , Rauhut Guntram
Published: January 2021

From an astrochemical point of view ketenimine (CH2CNH) is a complex organic molecule (COM) and therefore likely to be a building block for biologically relevant molecules. Since it has been detected in the star-forming region Sagittarius B2(N), it is of high relevance in this field. Although experimental data are available for certain bands, for some energy ranges such as above 1200 cm−1 reliable data virtually do not exist. In addition, high-level ab initio calculations are neither reported for ketenimine nor for one of its deuterated isotopologues. In this paper, we provide for the first time data from accurate quantum chemical calculations and a thorough analysis of the full rovibrational spectrum. Based on high-level potential energy surfaces obtained from explicitly correlated coupled-cluster calculations including up to 4-mode coupling terms, the (ro)vibrational spectrum of ketenimine has been studied in detail by variational calculations relying on rovibrational configuration interaction (RVCI) theory. Strong Fermi resonances were found for all isotopologues. Rovibrational infrared intensities have been obtained from dipole moment surfaces determined from the distinguishable cluster approximation. A comparison of the spectra of the CH2CNH molecule with experimental data validates our results, but also reveals new insight about the system, which shows very strong Coriolis coupling effects.
Relevance of Immune Infiltration and Clinical Outcomes in Pancreatic Ductal Adenocarcinoma Subtypes
Authors: Liu Rong , Liao Ya-Zhou , Zhang Wei , Zhou Hong-Hao
Published: January 2021

Purpose

Pancreatic ductal adenocarcinoma (PDAC) is a lethal cancer with high heterogeneity and dismal survival rates. Tumor immune microenvironment plays a critical role in sensitive to chemotherapy and prognosis. Herein, we determined the relevance of the composition of tumor-infiltrating immune cells to clinical outcomes in PDACs, and we evaluated these effects by molecular subtype.

Experimental Design

Data of 1,274 samples from publically available datasets were collected. Molecular subtypes were predicted with support vector machine. Twenty-two subsets of immune cells were estimated with CIBERSORTx. The associations between each cell subset and overall survival (OS), relapse free survival (RFS), and complete response (CR) to chemotherapy were evaluated, modelling cellular proportions as quartiles.

Results

An immune-related cluster was identified with unsupervised hierarchical clustering of hallmark pathways. Of the immune cells investigated, M0 macrophages emerged as closely associated with worse OS (HR =1.23, 95% CI = 1.15–1.31, p=1.57×10-9) and RFS (HR = 1.14, 95% CI =1.04–1.25, p=2.93×10-3), regardless of molecular subtypes. The CD8+ T cells conferred favorable survival. The neutrophils conferred poor OS overall (HR=1.17, 95% CI=1.10–1.23, p=1.74×10-7) and within the classical subtype. In the basal-like subtype, activated mast cells were associated with worse OS. Consensus clustering revealed six immune subgroups with distinct survival patterns and CR rates. The higher expression of PD1 was associated with better OS.

Conclusions

The immune cellular composition infiltrate in PDAC are likely to have effects on prognosis. Further exploration of the cellular immune response has the potential to identify candidates for immunotherapy.
Cardiac and Inflammatory Necrotizing Enterocolitis in Newborns Are Not the Same Entity
Authors: Klinke Michaela , Wiskemann Hanna , Bay Benjamin , Schäfer Hans-Jörg , Pagerols Raluy Laia , Reinshagen Konrad , Vincent Deirdre , Boettcher Michael
Published: January 2021

Background: Necrotizing enterocolitis (NEC) is an often-fatal neonatal disease involving intestinal hyperinflammation leading to necrosis. Despite ongoing research, (1) conflicting results and (2) comorbidities of NEC patients make early NEC detection challenging and may complicate therapy development. Most research suggests that NEC pathogenesis is multifactorial, involving a combination of (1) gut prematurity; (2) abnormal bacterial colonization; and (3) ischemia-reperfusion (I/R) injury. As neutrophil extracellular traps (NETs) partially mediate I/R injury and drive inflammation in NEC, we hypothesized that NETs contribute to NEC development; particularly in cardiac patients.

Methods: A retrospective analysis of baseline characteristics, clinical signs, laboratory parameters, and imaging was conducted for surgically verified NEC cases over 10 years. Patients were stratified into two groups: (1) prior medically or surgically treated cardiac disease (cardiac NEC) and (2) no cardiac comorbidities (inflammatory NEC). Additionally, histology was reassessed for neutrophil activation and NETs formation.

Results: A total of 110 patients (cNEC 43/110 vs. iNEC 67/110) were included in the study, with cNEC neonates being significantly older than iNEC neonates (p = 0.005). While no significant differences were found regarding clinical signs and imaging, laboratory parameters revealed that cNEC patients have significantly increased leucocyte (p = 0.024) and neutrophil (p < 0.001) counts. Both groups also differed in pH value (p = 0.011). Regarding histology: a non-significant increase in staining of myeloperoxidase within the cNEC group could be found in comparison to iNEC samples. Neutrophil elastase (p = 0.012) and citrullinated histone H3 stained (p = 0.041) slides showed a significant markup for neonates diagnosed with cNEC in comparison to neonates with iNEC.

Conclusion: The study shows that many standardized methods for diagnosing NEC are rather unspecific. However, differing leucocyte and neutrophil concentrations for iNEC and cNEC may indicate a different pathogenesis and may aid in diagnosis. As we propose that iNEC is grounded rather in sepsis and neutropenia, while cNEC primarily involves I/R injuries, which involves neutrophilia and NETs formation, it is plausible that I/R injury due to interventions for cardiac comorbidities results in pronounced neutrophil activation followed by a hyperinflammation reaction and NEC. However, prospective studies are necessary to validate these findings and to determine the accuracy of the potential diagnostic parameters.
Autoregulatory “Multitasking” at Endothelial Cell Junctions by Junction-Associated Intermittent Lamellipodia Controls Barrier Properties
Authors: Seebach Jochen , Klusmeier Nadine , Schnittler Hans
Published: January 2021

Vascular endothelial cell (EC) junctions are key structures controlling tissue homeostasis in physiology. In the last three decades, excellent studies have addressed many aspects of this complex and highly dynamic regulation, including cell signaling, remodeling processes of the proteins of tight junctions, adherens junctions, and gap junctions, the cytoskeleton, and post-transcriptional modifications, transcriptional activation, and gene silencing. In this dynamic process, vascular endothelial cadherin (VE-cadherin) provides the core structure of EC junctions mediating the physical adhesion of cells as well as the control of barrier function and monolayer integrity via remodeling processes, regulation of protein expression and post-translational modifications. In recent years, research teams have documented locally restricted dynamics of EC junctions in which actin-driven protrusions in plasma membranes play a central role. In this regard, our research group showed that the dynamics of VE-cadherin is driven by small (1–5 μm) actin-mediated protrusions in plasma membranes that, due to this specific function, were named “junction-associated intermittent lamellipodia” (JAIL). JAIL form at overlapping, adjacent cells, and exactly at this site new VE-cadherin interactions occur, leading to new VE-cadherin adhesion sites, a process that restores weak or lost VE-cadherin adhesion. Mechanistically, JAIL formation occurs locally restricted (1–5 μm) and underlies autoregulation in which the local VE-cadherin concentration is an important parameter. A decrease in the local concentration of VE-cadherin stimulates JAIL formation, whereas an increase in the concentration of VE-cadherin blocks it. JAIL mediated VE-cadherin remodeling at the subjunctional level have been shown to be of crucial importance in angiogenesis, wound healing, and changes in permeability during inflammation. The concept of subjunctional regulation of EC junctions is strongly supported by permeability assays, which can be employed to quantify actin-driven subjunctional changes. In this brief review, we summarize and discuss the current knowledge and concepts of subjunctional regulation in the endothelium.
The Covid-19 Emergency and the Risk of Social Fragmentation in the Palermo Case
Authors: Siino Antonia Roberta
Published: January 2021

The global Covid-19 crisis has shown its impact on all the aspect of life: health, social, economic, and politic. The attention mainly reserved to the economic and politic aspect musts also take into account the risk of social fragmentation. In which way this emergency has impact on communities where social fragilities were already existing? In which way, social dynamics are changed by the fear of the pandemic and the physical isolation imposed by governments to contain it. In which way local associations and citizen are coping the new challenges imposed by this emergency? In this article, the Sicilian context will be explored with a specific view on the Palermo capital. This Italian region has a peculiar and complex social asset determined by interrelated socio-economic phenomena such as mafia, unemployment and poverty. Fortunately, the existence of problematic and historical social phenomena has to face with the constant activities organized by local associations, civil society and authorities to fight them. There is one element that must to be consider: a global event like a pandemic oblige to be careful to the new ways in which phenomena we are accustomed to can manifest. Inequalities and racial matter can be exacerbated even in a multicultural context like Palermo one if social balance is missing. New form of poverty and the higher number of people exposed to this risk can give to mafia-type organizations more instrument to establish their control on the territory. The same introduction of economic measures aiming to support people can be manipulated and have a distorted effect when concretely applied. It is a great challenge because the lack of a complete comprehension can determine a failure of the adopted measures and a lack of cohesion of the social tissue. Starting from the context definition, the analysis will explore the perspective and the experiences of associations working with people belonging to the weaker part of the community, thanks to semi-structured interviews to representatives of the main local associations as privileged witness. In this way, the analysis will try to highlight how local actors' activities and how dynamics of solidarity are influenced by the global Covid-19 phenomenon.
Walking on Tiptoes: Digital Pads Deserve Increased Attention When Scoring Footpad Dermatitis as an Animal Welfare Indicator in Turkeys
Authors: Stracke Jenny , Volkmann Nina , May Franziska , Döhring Stefanie , Kemper Nicole , Spindler Birgit
Published: January 2021

Animal welfare is one of the most challenging issues in modern farm animal husbandry. Animal welfare indicators can be used to monitor welfare on farms or at slaughterhouses, with footpad dermatitis (FPD) being one of the most important indicators used in turkeys. Up to now, the severity of FPD has been measured by evaluating the size of altered lesions on the metatarsal pad of birds. However, such lesions are not only found on the metatarsal pads, but alterations can also occur on the digital pads of the animals, the latter is not included in the European standard scoring systems for turkeys so far. The aim of the present study was to give a detailed outline of alterations on the digital pads of turkeys and associate their occurrence to a standardly used five-point scoring system, which is based on alterations of the metatarsal pad only. Therefore, pictures of 500 feet of turkeys from 16 flocks at the end of the fattening phase were taken, using an automatic camera system. Based on these pictures, alterations on the digits were scored according to different parameters (lesions, swellings, and number of affected digits). Furthermore, detailed measurements were conducted using an imaging software. Results were compared with a standardly used five-point scoring system (standard FPD scoring system), based on the metatarsal pad as reference. Results provide no equivalence in occurrence and severity of alterations on the metatarsal pads compared to those found on the digits. Pathologic alterations on the digits were already present at standard FPD scoring level 0; no differentiation became obvious between the higher scoring levels 2–4. Strong correlations were found when comparing percentage of alterations of the standard FPD scoring system to those of a system including alterations on the digits and the metatarsal pad, using the total foot as a reference (rp = 0.9, p < 0.001). This was the first study conducting a detailed analysis of alterations on the digits of turkeys. In conclusion, results of this study show that including the evaluation of alterations on digits could refine the present FPD scoring system, especially when using FPD as an animal welfare indicator.
Porcine Babesiosis Caused by Babesia sp. Suis in a Pot-Bellied Pig in South Africa
Authors: Avenant Alida , Park Janice Y. , Vorster Ilse , Mitchell Emily P. , Arenas-Gamboa Angela M.
Published: January 2021

Babesiosis is a worldwide, tick-borne disease of economic importance in livestock caused by Babesia spp., which are hemoparasitic piroplasms that target the host erythrocytes. Cattle, dogs, small ruminants, and wild ruminants are the species most commonly affected, while in cats, horses, and pigs, it is less frequently reported. Although babesiosis has been observed worldwide, porcine babesiosis remains an uncommon disease with a very limited number of cases reported. Here, we describe a case in a 12-year old pot-bellied pig from South Africa that died after a history of anorexia and reluctance to rise for 2 days. A complete necropsy, blood smear cytology, reverse line blot (RLB) hybridization and 18S rRNA sequencing were performed. Numerous Babesia spp. hemoparasites and a moderate regenerative anemia were identified on blood smear, and a urine dipstick test yielded 4+ heme. Diffuse icterus and splenomegaly were observed upon gross examination. Histopathology revealed hemoglobin casts within renal tubules and collecting ducts, pulmonary edema, splenic congestion, and intrahepatic cholestasis. BLASTN homology of the 18SrRNA sequence revealed a 100% identity to the published sequence of Babesia sp. Suis isolated from pigs in Italy. This case of babesiosis in a pig highlights the clinical manifestations and gross and pathological findings of porcine babesiosis.
Quasi-3D Aerodynamic Analysis Method for Blended-Wing-Body UAV Configurations
Authors: Panagiotou Pericles , Dimopoulos Thomas , Dimitriou Stylianos , Yakinthos Kyros
Published: January 2021

The current study presents a low-fidelity, quasi-3D aerodynamic analysis method for Blended-Wing-Body (BWB) Unmanned Aerial Vehicle (UAV) configurations. A tactical BWB UAV experimental prototype is used as a reference platform. The method utilizes 2D panel method analyses and theoretical aerodynamic calculations to rapidly compute lift and pitching moment coefficients. The philosophy and the underlying theoretical and semi-empirical equations of the proposed method are extensively described. Corrections related to control surfaces deflection and ground effect are also suggested, so that the BWB pitching stability and trimming calculations can be supported. The method is validated against low-fidelity 3D aerodynamic analysis methods and high-fidelity, Computational Fluid Dynamics (CFD) results for various BWB configurations. The validation procedures show that the proposed method is considerably more accurate than existing low-fidelity ones, can provide predictions for both lift and pitching moment coefficients and requires far less computational resources and time when compared to CFD modeling. Hence, it can serve as a valuable aerodynamics and stability analysis tool for BWB UAV configurations.
Divergent and Convergent Attitudes and Views of General Practitioners and Community Pharmacists to Collaboratively Implement Antimicrobial Stewardship Programs in Australia: A Nationwide Study
Authors: Saha Sajal K. , Kong David C. M. , Thursky Karin , Mazza Danielle
Published: January 2021

Setting up an interprofessional team for antimicrobial stewardship (AMS) to improve the quality and safety of antimicrobial use in primary care is essential but challenging. This study aimed to investigate the convergent and divergent attitudes and views of general practitioners (GPs) and community pharmacists (CPs) about AMS implementation and their perceived challenges of collaboration to design a GP–pharmacist collaborative AMS (GPPAS) model. Nationwide surveys of GPs and CPs across Australia were conducted January-October 2019. Chi square statistics and a theoretical framework were used for comparative analyses of quantitative and qualitative data, respectively. In total, 999 participants responded to the surveys with 15.4% (n = 386) response rates for GPs and 30.7% (n = 613) for CPs. GPs and CPs were aware about AMS however their interprofessional perceptions varied to the benefits of AMS programs. CPs indicated that they would need AMS training; significantly higher than GPs (GP vs. CP; 46.4% vs. 76.5%; p < 0.0001). GPs’ use of the Therapeutic Guideline Antibiotic was much higher than CPs (83.2% vs. 45.5%; p < 0.0001). No interprofessional difference was found in the very-limited use of patient information leaflets (p < 0.1162) and point-of-care tests (p < 0.7848). While CPs were more willing (p < 0.0001) to collaborate with GPs, both groups were convergent in views that policies that support GP–CP collaboration are needed to implement GPPAS strategies. GP–pharmacist collaborative group meetings (54.9% vs. 82.5%) and antimicrobial audit (46.1% vs. 86.5%) models were inter-professionally supported to optimise antimicrobial therapy, but an attitudinal divergence was significant (p < 0.001). The challenges towards GP–CP collaboration in AMS were identified by both at personal, logistical and organisational environment level. There are opportunities for GP–CP collaboration to improve AMS in Australian primary care. However, strengthening GP–pharmacy collaborative system structure and practice agreements is a priority to improve interprofessional trust, competencies, and communications for AMS and to establish a GPPAS model in future.
The Impact of Mitochondrial Fission-Stimulated ROS Production on Pro-Apoptotic Chemotherapy
Authors: Ježek Jan , Cooper Katrina F. , Strich Randy
Published: January 2021

Cancer is one of the world’s deadliest afflictions. Despite recent advances in diagnostic and surgical technologies, as well as improved treatments of some individual tumor types, there is currently no universal cure to prevent or impede the uncontrolled proliferation of malignant cells. Targeting tumors by inducing apoptosis is one of the pillars of cancer treatment. Changes in mitochondrial morphology precede intrinsic apoptosis, but mitochondrial dynamics has only recently been recognized as a viable pharmacological target. In many cancers, oncogenic transformation is accompanied by accumulation of elevated cellular levels of ROS leading to redox imbalance. Hence, a common chemotherapeutic strategy against such tumor types involves deploying pro-oxidant agents to increase ROS levels above an apoptotic death-inducing threshold. The aim of this chapter is to investigate the benefit of stimulating mitochondrial fission-dependent production of ROS for enhanced killing of solid tumors. The main question to be addressed is whether a sudden and abrupt change in mitochondrial shape toward the fragmented phenotype can be pharmacologically harnessed to trigger a burst of mitochondrial ROS sufficient to initiate apoptosis specifically in cancer cells but not in non-transformed healthy tissues.
Structural and Biochemical Characterization of a Cold-Active PMGL3 Esterase with Unusual Oligomeric Structure
Authors: Boyko Konstantin M. , Kryukova Mariya V. , Petrovskaya Lada E. , Kryukova Elena A. , Nikolaeva Alena Y. , Korzhenevsky Dmitry A. , Lomakina Galina Yu. , Novototskaya-Vlasova Ksenia A. , Rivkina Elizaveta M. , Dolgikh Dmitry A. , Kirpichnikov Mikhail P. , Popov Vladimir O.
Published: January 2021

The gene coding for a novel cold-active esterase PMGL3 was previously obtained from a Siberian permafrost metagenomic DNA library and expressed in Escherichia coli. We elucidated the 3D structure of the enzyme which belongs to the hormone-sensitive lipase (HSL) family. Similar to other bacterial HSLs, PMGL3 shares a canonical α/β hydrolase fold and is presumably a dimer in solution but, in addition to the dimer, it forms a tetrameric structure in a crystal and upon prolonged incubation at 4 °C. Detailed analysis demonstrated that the crystal tetramer of PMGL3 has a unique architecture compared to other known tetramers of the bacterial HSLs. To study the role of the specific residues comprising the tetramerization interface of PMGL3, several mutant variants were constructed. Size exclusion chromatography (SEC) analysis of D7N, E47Q, and K67A mutants demonstrated that they still contained a portion of tetrameric form after heat treatment, although its amount was significantly lower in D7N and K67A compared to the wild type. Moreover, the D7N and K67A mutants demonstrated a 40 and 60% increase in the half-life at 40 °C in comparison with the wild type protein. Km values of these mutants were similar to that of the wt PMGL3. However, the catalytic constants of the E47Q and K67A mutants were reduced by ~40%.
Future Hydrology of the Cryospheric Driven Lake Como Catchment in Italy under Climate Change Scenarios
Authors: Fuso Flavia , Casale Francesca , Giudici Federico , Bocchiola Daniele
Published: January 2021

We present an assessment of climate change impact on the hydrology of the Lago di Como lake catchment of Italy. On one side, the lake provides water for irrigation of the Po valley during summer, and on the other side its regulation is crucial to prevent flood risk, especially in fall and winter. The dynamics of lake Como are linked to the complex cryospheric hydrology of its Alpine contributing catchment, which is in turn expected to change radically under prospective global warming. The Poli-Hydro model is used here to simulate the cryospheric processes affecting the hydrology of this high-altitude catchment. We demonstrated the model’s accuracy against historical hydrological observations, available during 2002–2018. We then used four Representative Concentration Pathways scenarios, provided by three Global Circulation Models under the AR6 of IPCC, to project potential climate change until 2100. We thereby derived daily series of rainfall and temperature, to be used as inputs for hydrological simulations. The climate projections here highlight a substantial increase in temperature at the end of the century, between +0.61° and +5.96°, which would lead to a decrease in the total ice volume in the catchment, by −50% to −77%. Moreover, there would be a decrease in the contribution of snow melt to the annual lake inflow, and an increase in ice melt under the worst-case scenarios. Overall, the annual Lake inflows would increase during autumn and winter and would decrease in summer. Our study may provide a tool to help policy makers to henceforth evaluate adaptation strategies in the area.
Determining Ultrasound Arrival Time by HHT and Kurtosis in Wind Speed Measurement
Authors: Liu Shiyuan , Li Zhipeng , Wu Tong , Zhang Wei
Published: January 2021

The determination of ultrasonic echo signal onset time is the core of performing the time difference method to calculate wind speed. However, in practical cases, background noise makes precise determination extremely difficult. This paper carries out research on the accurate determination of onset time, exploring the advantages of an improved method based on the combination of Hilbert-Huang Transform (HHT) and high-order statistics (kurtosis). Performing Hilbert-Huang Transform to the received wave is aimed at determining a rough arrival time, around which a fixed size of data is extracted as initial sample to avoid a false pick. Then the fourth-order kurtosis of a smaller sample, extracted successively by a moving window from the initial sample, is calculated. The minimum point corresponds to the initial onset time. This approach was tested on a real ultrasonic echo signal dataset, acquired in a wind tunnel with an ultrasonic anemometer. The proposed method showed satisfying results in both ideal cases and low signal-to-noise ratio (SNR) environment, compared with traditional onset time determination approaches, including Akaike Information Criterion (AIC-picker), Short-term Average over Long-term Average (STA/LTA), and Teager-Kaiser energy operator (TKEO). The experimental results acquired by the HHT-kurtosis method demonstrated that the proposed method possesses a high accuracy.
Real-Time Hair Segmentation Using Mobile-Unet
Authors: Yoon Ho-Sub , Park Seong-Woo , Yoo Jang-Hee
Published: January 2021

We described a real-time hair segmentation method based on a fully convolutional network with the basic structure of an encoder–decoder. In one of the traditional computer vision techniques for hair segmentation, the mean shift and watershed methodologies suffer from inaccuracy and slow execution due to multi-step, complex image processing. It is also difficult to execute the process in real-time unless an optimization technique is applied to the partition. To solve this problem, we exploited Mobile-Unet using the U-Net segmentation model, which incorporates the optimization techniques of MobileNetV2. In experiments, hair segmentation accuracy was evaluated by different genders and races, and the average accuracy was 89.9%. By comparing the accuracy and execution speed of our model with those of other models in related studies, we confirmed that the proposed model achieved the same or better performance. As such, the results of hair segmentation can obtain hair information (style, color, length), which has a significant impact on human-robot interaction with people.
Advances in Meta-Heuristic Optimization Algorithms in Big Data Text Clustering
Authors: Abualigah Laith , Gandomi Amir H. , Elaziz Mohamed Abd , Hamad Husam Al , Omari Mahmoud , Alshinwan Mohammad , Khasawneh Ahmad M.
Published: January 2021

This paper presents a comprehensive survey of the meta-heuristic optimization algorithms on the text clustering applications and highlights its main procedures. These Artificial Intelligence (AI) algorithms are recognized as promising swarm intelligence methods due to their successful ability to solve machine learning problems, especially text clustering problems. This paper reviews all of the relevant literature on meta-heuristic-based text clustering applications, including many variants, such as basic, modified, hybridized, and multi-objective methods. As well, the main procedures of text clustering and critical discussions are given. Hence, this review reports its advantages and disadvantages and recommends potential future research paths. The main keywords that have been considered in this paper are text, clustering, meta-heuristic, optimization, and algorithm.
Mechanical and Dielectric Properties of Aligned Electrospun Fibers
Authors: Isaac Blesson , Taylor Robert M. , Reifsnider Kenneth
Published: January 2021

This review paper examines the current state-of-the-art in fabrication of aligned fibers via electrospinning techniques and the effects of these techniques on the mechanical and dielectric properties of electrospun fibers. Molecular orientation, system configuration to align fibers, and post-drawing treatment, like hot/cold drawing process, contribute to better specific strength and specific stiffness properties of nanofibers. The authors suggest that these improved, aligned nanofibers, when applied in composites, have better mechanical and dielectric properties for many structural and multifunctional applications, including advanced aerospace applications and energy storage devices. For these applications, most fiber alignment electrospinning research has focused on either mechanical property improvement or dielectric property improvement alone, but not both simultaneously. Relative to many other nanofiber formation techniques, the electrospinning technique exhibits superior nanofiber formation when considering cost and manufacturing complexity for many situations. Even though the dielectric property of pure nanofiber mat may not be of general interest, the analysis of the combined effect of mechanical and dielectric properties is relevant to the present analysis of improved and aligned nanofibers. A plethora of nanofibers, in particular, polyacrylonitrile (PAN) electrospun nanofibers, are discussed for their mechanical and dielectric properties. In addition, other types of electrospun nanofibers are explored for their mechanical and dielectric properties. An exploratory study by the author demonstrates the relationship between mechanical and dielectric properties for specimens obtained from a rotating mandrel horizontal setup.
Cellulose Nanofibrils/Xyloglucan Bio-Based Aerogels with Shape Recovery
Authors: Mandin Samuel , Moreau Samuel , Talantikite Malika , Novalès Bruno , Maigret Jean-Eudes , Cathala Bernard , Moreau Céline
Published: January 2021

Bio-based aerogels containing cellulose nanofibrils (CNFs) are promising materials due to the inherent physical properties of CNF. The high affinity of cellulose to plant hemicelluloses (xyloglucan, xylan, pectin) is also an opportunity to develop biomaterials with new properties. Here, we prepared aerogels from gelled dispersions of CNFs and xyloglucan (XG) at different ratios by using a freeze-casting procedure in unidirectional (UD) and non-directional (ND) manners. As showed by rheology analysis, CNF and CNF/XG dispersions behave as true gels. We investigated the impact of the freezing procedure and the gel’s composition on the microstructure and the water absorption properties. The introduction of XG greatly affects the microstructure of the aerogel from lamellar to cellular morphology. Bio-based aerogels showed high water absorption capacity with shape recovery after compression. The relation between morphology and aerogel compositions is discussed.
Structure and Validity of Questionnaire for Oral Frail Screening
Authors: Nomura Yoshiaki , Ishii Yoshimasa , Chiba Yota , Suzuki Shunsuke , Suzuki Akira , Suzuki Senichi , Morita Kenji , Tanabe Joji , Yamakawa Koji , Ishiwata Yasuo , Ishikawa Meu , Sogabe Kaoru , Kakuta Erika , Okada Ayako , Otsuka Ryoko , Hanada Nobuhiro
Published: January 2021

Oral frailty is defined as the mild decline in oral function and located at the early and reversible stage of frailty. Therefore, early detection and early treatment of oral frailty is very useful. Simple and easy questionnaires, such as an oral frailty checklist, have been widely used for the screening and enlightenment of oral frailty of the Japanese people. We evaluate the structure and validity of the oral frailty checklist. The questionnaire of oral frailty was distributed for the citizens more than 50 years old from December 2018 to January 2019. The structural validity of the questionnaire is analyzed by structural equation modeling (SEM). The characteristics of the items are analyzed by Item Response Theory (IRT). The data of 725 subjects (360 men, 359 women, 6 no answer, mean age 71.3 ± 9.05) are analyzed. The questionnaire consisted of three latent variables. Items of “Brushing teeth at least twice a day”, “Regular attendance of dental clinic”, and “Using denture”, had low discrimination ability. The questionnaire used in this study is a useful tool for the screening of oral frailty. However, its scoring system needs to be improved.
Construction of a Medical Resource Sharing Mechanism Based on Blockchain Technology: Evidence from the Medical Resource Imbalance of China
Authors: Liu Hu , Liu Yuxuan
Published: January 2021

Health equity is a very important part of social equity. The outbreak of the novel coronavirus pneumonia (COVID-19) in a short period of time exposed the problems existing in the allocation of medical resources and the response to major public health emergencies in China. By using Kernel density estimation and Data envelopment analysis (DEA), it is found that the allocation and imbalance of medical resources in China are greatly different among regions, and the polarization phenomenon is obvious. As an important part of the information technology system, blockchain technology is characterized by decentralization and non-tampering. It can realize sharing of medical resources through a mechanism of resource storage, circulation, supervision, and protection. The construction of a medical resource sharing mechanism under the condition of blockchain technology will greatly improve the degree of medical resource sharing, will narrow the differences in resource allocation between regions, and can effectively respond to an outbreak of major public health emergencies.
Female Sex and Outcomes after Endovascular Aneurysm Repair for Abdominal Aortic Aneurysm: A Propensity Score Matched Cohort Analysis
Authors: Behrendt Christian-Alexander , Kreutzburg Thea , Kuchenbecker Jenny , Panuccio Giuseppe , Dankhoff Mark , Spanos Konstantinos , Kouvelos George , Debus Sebastian , Peters Frederik , Kölbel Tilo
Published: January 2021

Objective: Previous studies have showed a potential disadvantage of female patients who underwent abdominal aortic aneurysm (AAA) repair. The current study aims to determine sex-specific perioperative and long-term outcomes using propensity score matched unselected nationwide health insurance claims data. Methods: Insurance claims from a large German fund were used, covering around 8% of the insured German population. Patients who underwent endovascular aortic repair (EVAR) for intact AAA from 1 January 2011 to 30 April 2017 were included in the cohort. A 1:2 female to male propensity score matching was applied to adjust for confounding variables. Perioperative and long-term outcomes after 5 years were determined using matching and regression methods. Results: Among a total of 3736 patients (19.3% females, mean 75 years) undergoing EVAR for intact AAA, we identified 1863 matched patients. Before matching, females were more likely to be previously diagnosed with hypothyroidism, electrolyte disorders, rheumatoid disorders, and depression, while males were more often diabetics. In the matched sample, 23.4% of the females and 25.8% of the males died during a median follow-up of 776 and 792 days, respectively. Perioperatively, females were more likely to exhibit acute limb ischemia (5.3% vs. 3.2%, p = 0.031) and major bleeding (22.0% vs. 15.9%, p = 0.001) before they were discharged to rehabilitation (5.5% vs. 1.5%, p < 0.001) when compared to males. No statistically significant difference in perioperative (odds ratio 1.12, 95% CI 0.54–2.16) or long-term mortality (hazard ratio 0.91, 95% CI 0.76–1.08) was observed between sexes. This was also true regarding aortic reintervention rates after 1 year (2.0% vs. 2.9%) and 5 years (10.9% vs. 8.1%). Conclusion: The current retrospective matched analysis of insurance claims revealed high early access-related morbidity in females when compared to their male counterparts. Short-term or long-term survival and reintervention outcomes were similar between sexes.
Asthma and Obesity: Two Diseases on the Rise and Bridged by Inflammation
Authors: Bantulà Marina , Roca-Ferrer Jordi , Arismendi Ebymar , Picado César
Published: January 2021

Asthma and obesity are two epidemics affecting the developed world. The relationship between obesity and both asthma and severe asthma appears to be weight-dependent, causal, partly genetic, and probably bidirectional. There are two distinct phenotypes: 1. Allergic asthma in children with obesity, which worsens a pre-existing asthma, and 2. An often non allergic, late-onset asthma developing as a consequence of obesity. In obesity, infiltration of adipose tissue by macrophages M1, together with an increased expression of multiple mediators that amplify and propagate inflammation, is considered as the culprit of obesity-related inflammation. Adipose tissue is an important source of adipokines, such as pro-inflammatory leptin, produced in excess in obesity, and adiponectin with anti-inflammatory effects with reduced synthesis. The inflammatory process also involves the synthesis of pro-inflammatory cytokines such as IL-1β, IL-6, TNFα, and TGFβ, which also contribute to asthma pathogenesis. In contrast, asthma pro-inflammatory cytokines such as IL-4, IL-5, IL-13, and IL-33 contribute to maintain the lean state. The resulting regulatory effects of the immunomodulatory pathways underlying both diseases have been hypothesized to be one of the mechanisms by which obesity increases asthma risk and severity. Reduction of weight by diet, exercise, or bariatric surgery reduces inflammatory activity and improves asthma and lung function.
Dynapenia and Low Cognition: A Cross-Sectional Association in Postmenopausal Women
Authors: Pasco Julie A. , Stuart Amanda L. , Sui Sophia X. , Holloway-Kew Kara L. , Hyde Natalie K. , Tembo Monica C. , Rufus-Membere Pamela , Kotowicz Mark A. , Williams Lana J.
Published: January 2021

Dynapenia is a key contributor to physical frailty. Cognitive impairment and dementia accompany frailty, yet links between skeletal muscle and neurocognition are poorly understood. We examined the cross-sectional relationship between lower limb muscle strength and global cognitive function. Participants were 127 women aged 51–87 years, from the Geelong Osteoporosis Study. Peak eccentric strength of the hip-flexors and hip abductors was determined using a hand-held dynamometer, and dynapenia identified as muscle strength t-scores < −1. Cognition was assessed using the Mini-Mental State Examination (MMSE), and MMSE scores below the median were rated as low. Associations between dynapenia and low cognition were examined using logistic regression models. Hip-flexor dynapenia was detected in 38 (71.7%) women with low cognition and 36 (48.7%) with good cognition (p = 0.009); for hip abductor dynapenia, the pattern was similar (21 (39.6%) vs. 9 (12.2%); p < 0.001). While the observed difference for hip-flexor strength was attenuated after adjusting for age and height (adjusted Odds Ratio (OR) 1.95, 95%CI 0.86–4.41), low cognition was nearly 4-fold more likely in association with hip abductor dynapenia (adjusted OR 3.76, 95%CI 1.44–9.83). No other confounders were identified. Our data suggest that low strength of the hip abductors and low cognition are associated and this could be a consequence of poor muscle function contributing to cognitive decline or vice versa. As muscle weakness is responsive to physical interventions, this warrants further investigation.
Microneedle Arrays Combined with Nanomedicine Approaches for Transdermal Delivery of Therapeutics
Authors: Alimardani Vahid , Abolmaali Samira Sadat , Yousefi Gholamhossein , Rahiminezhad Zahra , Abedi Mehdi , Tamaddon Alimohammad , Ahadian Samad
Published: January 2021

Organic and inorganic nanoparticles (NPs) have shown promising outcomes in transdermal drug delivery. NPs can not only enhance the skin penetration of small/biomacromolecule therapeutic agents but can also impart control over drug release or target impaired tissue. Thanks to their unique optical, photothermal, and superparamagnetic features, NPs have been also utilized for the treatment of skin disorders, imaging, and biosensing applications. Despite the widespread transdermal applications of NPs, their delivery across the stratum corneum, which is the main skin barrier, has remained challenging. Microneedle array (MN) technology has recently revealed promising outcomes in the delivery of various formulations, especially NPs to deliver both hydrophilic and hydrophobic therapeutic agents. The present work reviews the advancements in the application of MNs and NPs for an effective transdermal delivery of a wide range of therapeutics in cancer chemotherapy and immunotherapy, photothermal and photodynamic therapy, peptide/protein vaccination, and the gene therapy of various diseases. In addition, this paper provides an overall insight on MNs’ challenges and summarizes the recent achievements in clinical trials with future outlooks on the transdermal delivery of a wide range of nanomedicines.
Dental Treatments under General Anesthesia on Children with Special Health Care Needs Enrolled in the Spanish Dental Care Program
Authors: Pecci-Lloret María Pilar , Guerrero-Gironés Julia , López-González Belén , Rodríguez-Lozano Francisco Javier , Oñate-Cabrerizo Daniel , Oñate-Sánchez Ricardo E. , Pecci-Lloret Miguel R.
Published: January 2021

The purpose is to analyze the medical characteristics of children with special health care needs (CSHCN) recommended for dental treatment under general anesthesia (GA), postoperative complications, and dental treatment outcomes under the regulation of the Spanish Dental Care Program (PADI). 111 clinical records were selected. The study population was divided into three age groups. The quantitative data was specified as the mean ± SD. For the qualitative variables, the Chi-Square test was used. One-way ANOVA and Bonferroni tests were used to examine the effect of the “age group” and the number of treatment procedures. A total of 1473 treatment procedures were performed, of which 110 (7.5%) were cleanings, 898 (61%) were restorative procedures, 332 (21.7%) were extractions, 22 (1.6%) were endodontic treatments, 62 (4.2%) were pulpotomies, and 59 (4%) were stainless steel crowns. Regarding the mean number of incisor root canal treatments (RCT), age group 3 received a significantly higher mean number of incisor RCTs than age group 1 (p = 0.02). Age group 1 received a higher average of pulpotomies and stainless-steel crowns (p = 0.00) compared to groups 2 and 3. GA is a safe procedure for the dental treatment of CSHCN, with minimal postoperative complications, which should be included among dental public programs.
Effects of Ferulic Acid Esterase-Producing Lactic Acid Bacteria and Storage Temperature on the Fermentation Quality, In Vitro Digestibility and Phenolic Acid Extraction Yields of Sorghum (Sorghum bicolor L.) Silage
Authors: Xie Yixiao , Guo Jingui , Li Wenqi , Wu Zhe , Yu Zhu
Published: January 2021

Two lactic acid bacteria (LAB) strains with different ferulic acid esterase (FAE) activities were isolated: Lactobacillus farciminis (LF18) and Lactobacillus plantarum (LP23). The effects of these strains on the fermentation quality, in vitro digestibility and phenolic acid extraction yields of sorghum (Sorghum bicolor L.) silage were studied at 20, 30 and 40 °C. Sorghum was ensiled with no additive (control), LF18 or LP23 for 45 days. At 40 °C, the lactic acid content decreased, whereas the ammonia nitrogen (NH3-N) content significantly increased (p < 0.05). At all three temperatures, the inoculants significantly improved the lactic acid contents and reduced the NH3-N contents (p < 0.05). Neither LP23 nor LF18 significantly improved the digestibility of sorghum silages (p > 0.05). The LP23 group exhibited higher phenolic acid extraction yields at 30 °C (p < 0.05), and the corresponding yields of the LF18 and control groups were improved at 40 °C (p < 0.05). FAE-producing LABs might partially ameliorate the negative effects of high temperature and improve the fermentation quality of sorghum silage. The screened FAE-producing LABs could be candidate strains for preserving sorghum silage at high temperature, and some further insights into the relationship between FAE-producing LABs and ensiling temperatures were obtained.
First Detection of Meloidogyne luci (Nematoda: Meloidogynidae) Parasitizing Potato in the Azores, Portugal
Authors: Rusinque Leidy , Nóbrega Filomena , Cordeiro Laura , Serra Clara , Inácio Maria L.
Published: January 2021

Potato is the third most important crop in the world after rice and wheat, with a great social and economic importance in Portugal as it is grown throughout the country, including the archipelagos of Madeira and the Azores. The tropical root-knot nematode (RKN) Meloidogyne luci is a polyphagous species with many of its host plants having economic importance and the ability to survive in temperate regions, which pose a risk to agricultural production. In 2019, M. luci was detected from soil samples collected from the council of Santo António in Pico Island (Azores). Bioassays were carried out to obtain females, egg masses, and second-stage juveniles to characterize this isolate morphologically, biochemically, and molecularly. The observed morphological features and morphometrics showed high similarity and consistency with previous descriptions. Concerning the biochemical characterization, the esterase (EST) phenotype displayed a pattern with three bands similar to the one previously described for M. luci and distinct from M. ethiopica. Regarding the molecular analysis, an 1800 bp region of the mitochondrial DNA between cytochrome oxidase subunit II (COII) and 16S rRNA genes was analyzed and the phylogenetic tree revealed that the isolate grouped with M. luci isolates (99.17%). This is the first report of M. luci parasitizing potato in the Azores islands, contributing additional information on the distribution of this plant-parasitic nematode.
Optimal Production and Inventory Policy in a Multiproduct Bakery Unit
Authors: Duarte Belmiro P. M. , Gonçalves André M. M. , Santos Lino O.
Published: January 2021

The problem of finding optimal production and inventory policies is crucial for companies of the food industry, especially those processing multiple products. Since companies are required to adopt the most efficient solutions to prosper, the operation at these optimal conditions can have an extensive impact on profit, resource allocation and product quality. We address the problem of finding the optimal production and inventory policy in a multiproduct bakery unit for two contexts: (i) deterministic consumption without inventory control; and (ii) stochastic consumption combined with delayed inventory control. A formulation is proposed for each of these two setups. The restrictions considered in the model framework are related to workforce availability, and the cost structure includes four components: (i) production cost; (ii) inventory cost; (iii) setup cost; and (iv) the cost due to the degradation of perceived quality. The problem is formulated as a Mixed Integer Linear Programming one and solved with a branch and cut algorithm-based solver. The formulation is applied to a real bakery unit producing a mix of eight products. Distinct demand and inventory lower levels are used for building scenarios to test both models and characterize the economic performance of the multiproduct bakery unit.
The relationship between corporate profitability and ESG performance with GMM-IV method
Authors: Fain Máté
Published: January 2021

This study aims to find the answer to the question how the ESG performance of the companies in the MSCI ACWI Index affects short-term profitability. The dependent variables of the analysis are the return on sales and the ROC/WACC ratio measuring added value. The ESG scores are the S&P Global SAM ratings. As the goal of the study is to assess the short-term effects, the 2019 profitability-ESG relation is examined along with several control variables. In studies analysing sustainability, measurement, specification errors and endogeneity are frequent which we manage by applying GMM procedure with robust instrumental variables. Based on our calculations, the relationship between financial and ESG performance is mostly neutral in the specific industries, which may help the achievement of the sustainable development goals of the United Nations in the long run.