bluuebunny/crossref_metadata_2025 huggingface dataset:
Maternal, Perinatal and Neonatal Outcomes of Triplet Pregnancies According to Chorionicity: Our 15-Year Experience in a Tertiary-Level Center
Authors: Bernal Claverol Mireia , Aracil Moreno Irene , Ruiz Minaya María , Fernández Muñoz María , Reyes Angullo Zurine Raquel , González Navarro Pablo , García-Honduvilla Natalio , Ortega Miguel A. , García Tizón Santiago , Pintado-Recarte María P. , de León-Luis Juan A.
Published: March 2024

Introduction: The goal of this study was to evaluate the effect of chorionicity on maternal, fetal and neonatal morbidity and mortality in triplet pregnancies in our environment. Methods: A retrospective observational study was carried out on triplet pregnancies that were delivered in a tertiary center between 2006 and 2020. A total of 76 pregnant women, 228 fetuses and 226 live newborns were analyzed. Of these triplet pregnancies, half were non-trichorionic. We analyzed maternal characteristics and obstetric, fetal, perinatal and neonatal complications based on their chorionicity, comparing trichorionic vs. non-trichorionic triplet pregnancies. Prematurity was defined as <34 weeks. We measured perinatal and neonatal mortality, composite neonatal morbidity and composite maternal morbidity. Results: Newborns with a monochorionic component had a lower gestational age at birth, presented greater prematurity under 34 weeks, lower birth weight, greater probability of birth weight under 2000 g and an APGAR score below 7 at 5 min after birth, more respiratory distress syndrome and, overall, higher composite neonatal morbidity. The monochorionic component of triple pregnancies may entail the development of complications intrinsic to shared circulation and require premature elective termination. This greater prematurity is also associated with a lower birth weight and to the main neonatal complications observed. These findings are in line with those that were previously published in the meta-analysis by our research group and previous literature. Discussion: Triplet gestations with a monochorionic component present a higher risk of obstetric, fetal and neonatal morbidity and mortality.
Shear Stress Quantification in Tissue Engineering Bioreactor Heart Valves: A Computational Approach
Authors: Dave Raj , Luraghi Giulia , Sierad Leslie , Migliavacca Francesco , Kung Ethan
Published: March 2024

Tissue-engineered heart valves can grow, repair, and remodel after implantation, presenting a more favorable long-term solution compared to mechanical and porcine valves. Achieving functional engineered valve tissue requires the maturation of human cells seeded onto valve scaffolds under favorable growth conditions in bioreactors. The mechanical stress and strain on developing valve tissue caused by different pressure and flow conditions in bioreactors are currently unknown. The aim of this study is to quantify the wall shear stress (WSS) magnitude in heart valve prostheses under different valve geometries and bioreactor flow rates. To achieve this, this study used fluid–structure interaction simulations to obtain the valve’s opening geometries during the systolic phase. These geometries were then used in computational fluid dynamics simulations with refined near-wall mesh elements and ranges of prescribed inlet flow rates. The data obtained included histograms and regression curves that characterized the distribution, peak, and median WSS for various flow rates and valve opening configurations. This study also found that the upper region of the valve near the commissures experienced higher WSS magnitudes than the rest of the valve.
Research on Ship Automatic Berthing Algorithm Based on Flow Matching and Velocity Matching
Authors: Zhang Yi , Zhao Hengchao , Zhang Zheng , Wang Hongbo
Published: March 2024

Addressing the automatic berthing task for vessels, this study introduces the Flow Matching Double Section Bezier Berth Method (FM-DSB) for handling downstream and upstream berthing instructions. By considering the orientation relationship between the direction of water flow and the berth, combined with berthing modes, the algorithm determines the vessel’s entry angle into the berth and plans the berthing path using double-section Bezier curves. Effective control of vessel speed post-path determination is essential. Therefore, based on the response of vessels to propeller inputs, this study introduces the Berthing Path Velocity Matching Method (BPVM). The BPVM ensures speed matching along the berthing path through analysis of vessel acceleration and deceleration capabilities. Subsequently, simulation experiments are conducted to validate the planning algorithm for both long-distance and short-distance berthing. Furthermore, the feasibility and effectiveness of the berthing path are verified using a dual-loop path tracker based on the planned results. Experimental outcomes illustrate the adaptability of the proposed algorithm in planning berthing paths that align with vessel motion characteristics, effectively guiding vessels into berths through the designed dual-loop control system.
Too Hot to Handle: Effects of Water Temperature on the Early Life Stages of Gongolaria barbata (Fucales)
Authors: Lokovšek Ana , Pitacco Valentina , Falace Annalisa , Trkov Domen , Orlando-Bonaca Martina
Published: March 2024

Gongolaria barbata plays a crucial role as a habitat-forming Fucales species in the Mediterranean Sea, thriving in shallow, sheltered coastal regions, where it exhibits optimal growth in a temperature range of 10 to 25 °C. In the northern Adriatic Sea, a semi-enclosed part of the Mediterranean, there has been a remarkable increase in seawater temperatures in recent decades, often exceeding 28 °C in summer. These high temperatures pose a significant threat to the vulnerable early life stages of G. barbata. This study delves into the effects of four temperatures (15, 18, 24, and 28 °C) on the growth of G. barbata over its first 16 days, closely monitoring mortality, deformities, and overall survival. Our experiments reveal that higher temperatures can result in deformities and increased mortality of germlings. Notably, a temperature of 28 °C resulted in the death of all germlings within the first week, whereas those exposed to 24 °C survived until the second week, albeit with significant deformities prior to death. In contrast, germlings cultivated at 15 and 18 °C exhibited normal development with minimal deformities. These results highlight the susceptibility of the early life stages of G. barbata to temperature-induced stress and provide valuable insights into the potential consequences of rising seawater temperatures in the Mediterranean.
Systemic Candida Infection and Pulmonary Aspergillosis in an Alpaca (Vicugna pacos): A Case Report
Authors: Grassi Andrea , Cafarchia Claudia , Decaro Nicola , Rhimi Wafa , De Laurentiis Vittoriana , D’Annunzio Giulia , Luppi Andrea , Prati Paola
Published: March 2024

This study reports a peculiar case of systemic candidiasis infection associated with pulmonary aspergillosis in an apparently immunocompetent alpaca. A captive 7-year-old female alpaca exhibited respiratory symptoms, underwent treatment with benzylpenicillin and dexamethasone, and succumbed to the infection 40 days later. During the post-mortem examination, subcutaneous emphysema, widespread pneumonia with multiple suppurative foci, scattered necro-suppurative lesions throughout the renal and hepatic parenchyma were evident. Histopathological analysis of the collected tissues revealed multifocal mild lymphoplasmacytic chronic interstitial nephritis, necro-suppurative pneumonia with the presence of fungal hyphae, multifocal foci of mineralization, and fibrosis in the liver. Fungal cultures confirmed the growth of Aspergillus fumigatus from the lungs, and Candida albicans from the liver, kidney, and heart. The only recognizable risk factor for candidiasis and pulmonary aspergillosis in this case was prior corticosteroid and antibiotic therapy. Nevertheless, it is crucial to consider systemic candidosis and pulmonary aspergillosis as potential differential diagnoses in respiratory infections among camelids. Prolonged treatment with glucocorticoids and antibiotics should be avoided as it could represent a risk factor for the onset of pathologies caused by opportunistic fungi such as Candida spp. and Aspergillus spp.
Opioids in Treatment of Refractory Dyspnea in Chronic Obstructive Pulmonary Disease: Yes, No or Maybe
Authors: Rajnoveanu Ruxandra-Mioara , Harangus Antonia , Todea Doina Adina , Man Milena Adina , Budin Corina Eugenia , Rajnoveanu Armand-Gabriel
Published: March 2024

Chronic Obstructive Pulmonary Disease (COPD) is a complex condition with significant impact on prognosis, especially in advanced stages where symptom burden becomes critical. Breathlessness affects patients’ quality of life, and despite various therapeutic strategies, the role of opioids in palliative care for COPD remains under investigation. The acceptance of a therapeutic trial of different types of opioids is increasing not only in end-of-life situations but also for stable COPD patients experiencing intolerable refractory breathlessness despite optimal conventional therapy. Recent clinical trials have raised questions about the overall clinical benefit of opioids in addressing breathlessness in COPD, prompting the need to clarify inconsistencies and identify specific subgroups that may benefit from opioid therapy. In the clinical setting, it is crucial to understand the attributes of patients who exhibit positive responses to opioids and what type of opioids could have a positive impact. This research paper aims to offer an update of the most recent evidence of opioid treatment in managing breathlessness among individuals with COPD with a head-to-head evaluation of the supporting and opposing proof in the medical literature.
Genetic Factors Contributing to the Pathogenesis of Essential Hypertension in Two African Populations
Authors: Kalideen Kusha , Rayner Brian , Ramesar Raj
Published: March 2024

The African continent has the highest prevalence of hypertension globally, with South Africa reporting the highest prevalence in Southern Africa. While the influence of genetic variability in the pathogenesis of hypertension is well described internationally, limited reports are available for African populations. This study aimed to assess the association of genetic variants and essential hypertension in a cohort of two ethnic South African population groups. Two hundred and seventy-seven hypertensive and one hundred and seventy-six normotensive individuals were genotyped for 78 variants. Genotyping was performed using the Illumina GoldenGate Assay and allele-specific polymerase chain reaction. The association of variants was assessed using the Fisher Exact test under the additive and allelic genetic models, while multivariate logistic regression was used to predict the development of hypertension. Five variants (CYP11B2 rs179998, AGT rs5051 and rs699, AGTR1 rs5186, and ACE rs4646994) were significantly associated with essential hypertension in the cohort under study. Furthermore, AGTR1 rs5186 and AGT rs699 were identified as risk factors for the development of hypertension in both ethnic groups. In two ethnic South African populations, an association was observed between renin–angiotensin–aldosterone system (RAAS)-related genes and the development of hypertension.
Extracellular Vesicles as Next-Generation Biomarkers in Lung Cancer Patients: A Case Report on Adenocarcinoma and Squamous Cell Carcinoma
Authors: Ruzycka-Ayoush Monika , Prochorec-Sobieszek Monika , Cieszanowski Andrzej , Glogowski Maciej , Szumera-Cieckiewicz Anna , Podgorska Joanna , Targonska Alicja , Sobczak Kamil , Mosieniak Grazyna , Grudzinski Ireneusz P.
Published: March 2024

Extracellular vesicles (EVs) released from primary cell lines, originating from resected tissues during biopsies in patients with non-small cell lung cancer (NSCLC) revealing adenocarcinoma and squamous cell carcinoma subtypes, were examined for membrane proteomic fingerprints using a proximity barcoding assay. All the collected EVs expressed canonical tetraspanins (CD9, CD63, and CD81) highly coexpressed with molecules such as lysosome-associated membrane protein-1 (LAMP1–CD107a), sialomucin core protein 24 (CD164), Raph blood group (CD151), and integrins (ITGB1 and ITGA2). This representation of the protein molecules on the EV surface may provide valuable information on NSCLC subtypes and offer new diagnostic opportunities as next-generation biomarkers in personalized oncology.
Biodistribution Assessment of a Novel 68Ga-Labeled Radiopharmaceutical in a Cancer Overexpressing CCK2R Mouse Model: Conventional and Radiomics Methods for Analysis
Authors: Pavone Anna Maria , Benfante Viviana , Giaccone Paolo , Stefano Alessandro , Torrisi Filippo , Russo Vincenzo , Serafini Davide , Richiusa Selene , Pometti Marco , Scopelliti Fabrizio , Ippolito Massimo , Giannone Antonino Giulio , Cabibi Daniela , Asti Mattia , Vettorato Elisa , Morselli Luca , Merone Mario , Lunardon Marcello , Andrighetto Alberto , Tuttolomondo Antonino , Cammarata Francesco Paolo , Verona Marco , Marzaro Giovanni , Mastrotto Francesca , Parenti Rosalba , Russo Giorgio , Comelli Albert
Published: March 2024

The aim of the present study consists of the evaluation of the biodistribution of a novel 68Ga-labeled radiopharmaceutical, [68Ga]Ga-NODAGA-Z360, injected into Balb/c nude mice through histopathological analysis on bioptic samples and radiomics analysis of positron emission tomography/computed tomography (PET/CT) images. The 68Ga-labeled radiopharmaceutical was designed to specifically bind to the cholecystokinin receptor (CCK2R). This receptor, naturally present in healthy tissues such as the stomach, is a biomarker for numerous tumors when overexpressed. In this experiment, Balb/c nude mice were xenografted with a human epidermoid carcinoma A431 cell line (A431 WT) and overexpressing CCK2R (A431 CCK2R+), while controls received a wild-type cell line. PET images were processed, segmented after atlas-based co-registration and, consequently, 112 radiomics features were extracted for each investigated organ / tissue. To confirm the histopathology at the tissue level and correlate it with the degree of PET uptake, the studies were supported by digital pathology. As a result of the analyses, the differences in radiomics features in different body districts confirmed the correct targeting of the radiopharmaceutical. In preclinical imaging, the methodology confirms the importance of a decision-support system based on artificial intelligence algorithms for the assessment of radiopharmaceutical biodistribution.
Aerobic Exercise Modulates Proteomic Profiles in Gastrocnemius Muscle of db/db Mice, Ameliorating Sarcopenia
Authors: Huang Yen-Chun , Sanotra Monika Renuka , Huang Chi-Chang , Hsu Yi-Ju , Liao Chen-Chung
Published: March 2024

Type-2 diabetes mellitus (T2DM)-induced sarcopenia is intertwined with diminished insulin sensitivity and extracellular matrix (ECM) remodeling in skeletal muscle and other organs. Physical activities such as aerobic exercise play a crucial role in regulating blood glucose levels, insulin sensitivity, metabolic pathways, oxidative stress, fibrosis, ECM remodeling, and muscle regeneration by modulating differentially expressed protein (DEP) levels. The objectives of our research were to investigate the effect of six weeks of aerobic exercise on the gastrocnemius and soleus muscle of db/db mice’s DEP levels compared to those of sedentary db/db mice. A total of eight db/db mice were divided into two groups (n = 4 per group), namely sedentary mice (SED) and exercise-trained mice (ET), of which the latter were subjected to six weeks of a moderate-intensity aerobic exercise intervention for five days per week. After the exercise intervention, biochemical tests, including analyses of blood glucose and HbA1c levels, were performed. Histological analysis using H & E staining on tissue was performed to compare morphological characters. Gastrocnemius and soleus muscles were dissected and processed for proteomic analysis. Data were provided and analyzed based on the DEPs using the label-free quantification (LFQ) algorithm. Functional enrichment analysis and Ingenuity Pathway Analysis (IPA) were employed as bioinformatics tools to elucidate the molecular mechanisms involved in the DEPs and disease progression. Significantly reduced blood glucose and HbA1c levels and an increased cross-sectional area (CSA) of gastrocnemius muscle fibers were seen in the ET group after the exercise interventions due to upregulations of metabolic pathways. Using proteomics data analysis, we found a significant decrease in COL1A1, COL4A2, ENG, and LAMA4 protein levels in the ET gastrocnemius, showing a significant improvement in fibrosis recovery, ECM remodeling, and muscle regeneration via the downregulation of the TGF-β signaling pathway. Upregulated metabolic pathways due to ET-regulated DEPs in the gastrocnemius indicated increased glucose metabolism, lipid metabolism, muscle regeneration, and insulin sensitivity, which play a crucial role in muscle regeneration and maintaining blood glucose and lipid levels. No significant changes were observed in the soleus muscle due to the type of exercise and muscle fiber composition. Our research suggests that engaging in six weeks of aerobic exercise may have a positive impact on the recovery of T2DM-induced sarcopenia, which might be a potential candidate for mitigation, prevention, and therapeutic treatment in the future.
Profiling Social Sentiment in Times of Health Emergencies with Information from Social Networks and Official Statistics
Authors: Velasco-López Jorge-Eusebio , Carrasco Ramón-Alberto , Serrano-Guerrero Jesús , Chiclana Francisco
Published: March 2024

Social networks and official statistics have become vital sources of information in times of health emergencies. The ability to monitor and profile social sentiment is essential for understanding public perception and response in the context of public health crises, such as the one resulting from the COVID-19 pandemic. This study will explore how social sentiment monitoring and profiling can be conducted using information from social networks and official statistics, and how this combination of data can offer a more complete picture of social dynamics in times of emergency, providing a valuable tool for understanding public perception and guiding a public health response. To this end, a three-layer architecture based on Big Data and Artificial Intelligence is presented: the first layer focuses mainly on collecting, storing, and governing the necessary data such as social media and official statistics; in the second layer, the representation models and machine learning necessary for knowledge generation are built, and in the third layer the previously generated knowledge is adapted for better understanding by crisis managers through visualization techniques among others. Based on this architecture, a KDD (Knowledge Discovery in Databases) framework is implemented using methodological tools such as sentiment analysis, fuzzy 2-tuple linguistic models and time series prediction with the Prophet model. As a practical demonstration of the proposed model, we use tweets as data source (from the social network X, formerly known as Twitter) generated during the COVID-19 pandemic lockdown period in Spain, which are processed to identify the overall sentiment using sentiment analysis techniques and fuzzy linguistic variables, and combined with official statistical indicators for prediction, visualizing the results through dashboards.
A Divestment Model: Migration to Green Energy Investment Portfolio Concept
Authors: Moagi Gaoganwe Sophie , Doctor Obonye , Lungu Edward
Published: March 2024

In a targeted terminal wealth generated by bond and risky assets, where the proportion of a risky asset is gradually being phased down, we propose a divestment model in a risky asset compensated by growth in a bond (insurance). The model includes the phase-down rate of the risky asset, c(t), the variable proportion, π(t), in a risky asset and the interest rate, r, of the bond. To guide the growth of the total wealth in this study, we compared it to the Øksendal and Sulem (Backward Stochastic Differential Equations and Risk Measures (2019)) total wealth for which c(t)=0, and π(t) is a constant. We employed the Fokker–Planck equation to find the variable moment, π(t), and the associated variance. We proved the existence and uniqueness of the first moment by Feller’s criteria. We have found a pair (c*(t),r*) for each π(t), which guarantees a growing total wealth. We have addressed the question whether this pair can reasonably be achieved to ensure an acceptable phase-down rate at a financially achievable interest rate, r*.
An Untargeted Metabolomics Strategy to Identify Substrates of Known and Orphan E. coli Transporters
Authors: Radi Mohammad S. , Munro Lachlan J. , Rago Daniela , Kell Douglas B.
Published: March 2024

Transport systems play a pivotal role in bacterial physiology and represent potential targets for medical and biotechnological applications. However, even in well-studied organisms like Escherichia coli, a notable proportion of transporters, exceeding as many as 30%, remain classified as orphans due to their lack of known substrates. This study leveraged high-resolution LC-MS-based untargeted metabolomics to identify candidate substrates for these orphan transporters. Human serum, including a diverse array of biologically relevant molecules, served as an unbiased source for substrate exposure. The analysis encompassed 26 paired transporter mutant contrasts (i.e., knockout vs. overexpression), compared with the wild type, revealing distinct patterns of substrate uptake and excretion across various mutants. The convergence of candidate substrates across mutant scenarios provided robust validation, shedding light on novel transporter-substrate relationships, including those involving yeaV, hsrA, ydjE, and yddA. Furthermore, several substrates were contingent upon the specific mutants employed. This investigation underscores the utility of untargeted metabolomics for substrate identification in the absence of prior knowledge and lays the groundwork for subsequent validation experiments, holding significant implications for both medical and biotechnological advancements.
A Study on the Aging Behavior of Nitrided W18Cr4V Steel in High-Temperature Sodium
Authors: Fu Xiaogang , Liang Na , Zhang Wei , Tao Liu , Qin Bo , Ruan Zhangshun , Long Bin , Lv Shasha
Published: March 2024

The loading and unloading elevators are the primary equipment in the refueling system, used for transferring fuel assemblies in the sodium-cooled fast reactors. The guideway friction pairs are the critical components of these elevators in the refueling system. With the excellent hardness and wear resistance in air, nitrided W18Cr4V steel is a promising material for the guideway friction pairs. In order to assess the feasibility of using nitrided W18Cr4V steel, it is essential to understand the aging behavior of nitrided W18Cr4V steel in high-temperature sodium. Aging tests were conducted on nitrided W18Cr4V steel in sodium and in argon environments at various temperatures for different exposure times. The results showed that the nitrogen atoms in the nitrided layer exhibited bidirectional diffusion behavior in the sodium or argon environment at 540 °C. Compared to the argon environment, cracks formed within the nitrided layer and the diffusion of nitrogen into the sodium was accelerated in the nitrided layer. As a significant number of nitrogen atoms had diffused into the sodium, there was little difference in the hardness between nitrided W18Cr4V steel and non-nitrided W18Cr4V steel after long-term exposure to 540 °C sodium.
Morphological Evolution of Single-Core Multi-Strand Wires during Ultrasonic Metal Welding
Authors: Gester Andreas , Ozherelkov Dmitrii , Wagner Guntram
Published: March 2024

Ultrasonic metal welding (USMW) finds widespread utilization in automotive industries, where it is used for connecting the wire harness of the vehicle, consisting of stranded wires, to the terminals. However, the behavior of the strands during the compaction process is still understudied and often overlooked. Therefore, this work focuses on the investigation of the wire compaction behavior from a morphological point of view. A newly developed method for investigating cross-sections of such joints is introduced, facilitating area quantification of the strands for a microscale examination of compaction variations for every single strand as a function of welding time. It is shown that the deformation in the wire is not homogenous throughout the wire cross-section; instead, the formation of distinct zones is observed. Three distinct regimes dominating the welding process were observed: (i) linear reduction in nugget height with primary compaction of the nugget and sealing of the interstitial spaces between the strands for weld times from 0 s up to 1.3 s; (ii) accelerated loss of nugget height due to strong plastic deformation of the strands for weld times between 1.3 s and 1.7 s; and (iii) comprehensive welding of the individual strands and strong loss of nugget height. Furthermore, it was demonstrated that the deformation of the wire during the USMW process originates in the coupling area of the horn and the wire and not in the interface of the wire and the terminal. Therefore, it can be assumed that the temperature of the interface between the horn and the wire must be significantly higher than that of the interface between the wire and the terminal. The presented approach and new insights into the behavior of ultrasonically welded joints of stranded wires and terminals provide guidance for improving the welding process.
Vaginal Microbiota and HPV in Latin America: A Narrative Review
Authors: Tosado-Rodríguez Eduardo , Alvarado-Vélez Ian , Romaguera Josefina , Godoy-Vitorino Filipa
Published: March 2024

With the expansion of human microbiome studies in the last 15 years, we have realized the immense implications of microbes in human health. The human holobiont is now accepted, given the commensal relationships with bacteria, fungi, parasites, viruses, and human cells. The cervicovaginal microbiota is a specific case within the human microbiome where diversity is lower to maintain a chemical barrier of protection against infections. This narrative review focuses on the vaginal microbiome. It summarizes key findings on how native bacteria protect women from disease or predispose them to damaging inflammatory processes with an emphasis on the role of HPV infections in Latin America, one of the world’s regions with the highest cervical cancer prevalence.
Towards a Circular Economy in the Mining Industry: Possible Solutions for Water Recovery through Advanced Mineral Tailings Dewatering
Authors: Hamraoui Laila , Bergani Abdelilah , Ettoumi Mouna , Aboulaich Abdelmaula , Taha Yassine , Khalil Abdessamad , Neculita Carmen Mihaela , Benzaazoua Mostafa
Published: March 2024

The mining industry is confronted with substantial challenges in achieving environmental sustainability, particularly regarding water usage, waste management, and dam safety. The increasing global demand for minerals has led to increased mining activities, resulting in significant environmental consequences. By 2025, an estimated 19 billion tons of solid tailings are projected to accumulate worldwide, exacerbating concerns over their management. Tailings storage facilities represent the largest water sinks within mining operations. The mismanagement of water content in tailings can compromise their stability, leading to potential dam failures and environmental catastrophes. In response to these pressing challenges, the mining industry is increasingly turning to innovative solutions such as tailings dewatering and water reuse/recycling strategies to promote sustainable development. This review paper aims to (I) redefine the role of mine tailings and explore their physical, chemical, and mineralogical characteristics; (II) discuss environmental concerns associated with conventional disposal methods; (III) explore recent advancements in dewatering techniques, assessing their potential for water recovery, technical and economic constraints, and sustainability considerations; (IV) and present challenges encountered in water treatment and recycling within the mining industry, highlighting areas for future research and potential obstacles in maximizing the value of mine tailings while minimizing their environmental impact.
Diffusiophoresis of Macromolecules within the Framework of Multicomponent Diffusion
Authors: Annunziata Onofrio
Published: March 2024

Diffusiophoresis is the isothermal migration of a colloidal particle through a liquid caused by a cosolute concentration gradient. Although diffusiophoresis was originally introduced using hydrodynamics, it can also be described by employing the framework of multicomponent diffusion. This not only enables the extraction of diffusiophoresis coefficients from measured multicomponent-diffusion coefficients but also their theoretical interpretation using fundamental thermodynamic and transport parameters. This review discusses the connection of diffusiophoresis with the 2 × 2 diffusion-coefficient matrix of ternary liquid mixtures. Specifically, diffusiophoresis is linked to the cross-term diffusion coefficient characterizing diffusion of colloidal particles due to cosolute concentration gradient. The other cross-term, which describes cosolute diffusion due to the concentration gradient of colloidal particles, is denoted as osmotic diffusion. Representative experimental results on diffusiophoresis and osmotic diffusion for polyethylene glycol and lysozyme in the presence of aqueous salts and osmolytes are described. These data were extracted from ternary diffusion coefficients measured using precision Rayleigh interferometry at 25 °C. The preferential-hydration and electrophoretic mechanisms responsible for diffusiophoresis are examined. The connection of diffusiophoresis and osmotic diffusion to preferential-interaction coefficients, Onsager reciprocal relations, Donnan equilibrium and Nernst–Planck equations are also discussed.
Conductometric and Thermodynamic Studies of Selected Imidazolium Chloride Ionic Liquids in N,N-Dimethylformamide at Temperatures from 278.15 to 313.15 K
Authors: Kinart Zdzisław
Published: March 2024

This scientific article presents research on the electrical conductivity of imidazole-derived ionic liquids (1-methylimidazolium chloride, 1-ethyl-3-methylimidazolium chloride, 1-butyl-3-methylimidazolium chloride, 1-hexyl-3-methylimidazolium chloride and 1-methyl-3-octylimidazolium chloride) in the temperature range of 278.15–313.15 K in N,N-Dimethylformamide. The measurement methods employed relied mainly on conductometric measurements, enabling precise monitoring of the conductivity changes as a function of temperature. Experiments were conducted at various temperature values, which provided a comprehensive picture of the conducting properties of the investigated ionic liquids. The focus of the study was the analysis of the conductometric results, which were used to determine the conductivity function as a function of temperature. Based on the obtained data, a detailed analysis of association constants (KA) and thermodynamic parameters such as enthalpy (∆H0), entropy (∆S0), Gibbs free energy (∆G0), Eyring activation enthalpy for charge transport (ΔHλ‡) and diffusion processes (D0) was carried out. The conductometric method proved to be an extremely effective tool for accurately determining these parameters, significantly contributing to the understanding of the properties of imidazole-derived ionic liquids in the investigated temperature range. As a result, the obtained results not only provide new insights into the electrical conductivity of the studied ionic liquids but also broaden our knowledge of their thermodynamic behavior under different temperature conditions. These studies may have significant implications for the field of ionic liquid chemistry and may be applied in the design of modern materials with desired conducting properties.
Ethylene-alt-α-Olefin Copolymers by Hydrogenation of Highly Stereoregular cis-1,4 Polydienes: Synthesis and Structural Characterization
Authors: Ricci Giovanni , Boccia Antonella Caterina , Pierro Ivana , De Rosa Claudio , Scoti Miriam
Published: March 2024

The homogeneous non-catalytic hydrogenation of several types of iso- and syndiotactic cis-1,4 poly(1,3-diene)s with diimide, formed by thermal decomposition of p-toluene-sulfonyl-hydrazide, was examined. Perfectly alternating ethylene/1-alkene copolymers having different tacticity (i.e., isotactic and syndiotactic), which in some cases are difficult to synthesize by simple stereospecific co-polymerization of the corresponding monomers, were obtained. All the copolymers synthesized were fully characterized from a structural, morphological, and rheological point of view through different analytical techniques (FT-IR, NMR, GPC, DSC, RX).
Autoantibodies-Abzymes with Phosphatase Activity in Experimental Autoimmune Encephalomyelitis Mice
Authors: Urusov Andrey E. , Aulova Kseniya S. , Nevinsky Georgy A.
Published: March 2024

The exact mechanisms of MS (multiple sclerosis) evolution are still unknown. However, the development of EAE (experimental autoimmune encephalomyelitis simulating human MS) in C57BL/6 mice occurs due to the violation of bone marrow hematopoietic stem cell differentiation profiles, leading to the production of toxic for human autoantibody splitting MBP (myelin basic protein), MOG (mouse oligodendrocyte glycoprotein), five histones, DNA, and RNA. Here, we first analyzed the changes in the relative phosphatase activity of IgGs from C57BL/6 mice blood over time, corresponding to three stages of EAE: onset, acute, and remission. Antibodies have been shown to catalyze the hydrolysis of p-nitrophenyl phosphate at several optimal pH values, mainly in the range of 6.5–7.0 and 8.5–9.5. During the spontaneous development of EAE, the most optimal value is pH 6.5. At 50 days after the birth of mice, the phosphatase activity of IgGs at pH 8.8 is 1.6-fold higher than at pH 6.5. During spontaneous development of EAE from 50 to 100 days, an increase in phosphatase activity is observed at pH 6.5 but a decrease at pH 8.8. After mice were immunized with DNA–histone complex by 20 and 60 days, phosphatase activity increased respectively by 65.3 and 109.5 fold (pH 6.5) and 128.4 and 233.6 fold (pH 8.8). Treatment of mice with MOG at the acute phase of EAE development (20 days) leads to a maximal increase in the phosphatase activity of 117.6 fold (pH 6.5) and 494.7 fold (pH 8.8). The acceleration of EAE development after mice treatment with MOG and DNA–histone complex results in increased production of lymphocytes synthesizing antibodies with phosphatase activity. All data show that IgG phosphatase activity could be essential in EAE pathogenesis.
The Essential Oil Compositions of Ambrosia acanthicarpa Hook., Artemisia ludoviciana Nutt., and Gutierrezia sarothrae (Pursh) Britton & Rusby (Asteraceae) from the Owyhee Mountains of Idaho
Authors: Swor Kathy , Poudel Ambika , Satyal Prabodh , Setzer William N.
Published: March 2024

As part of our interest in the volatile phytoconstituents of aromatic plants of the Great Basin, we have obtained essential oils of Ambrosia acanthicarpa (three samples), Artemisia ludoviciana (12 samples), and Gutierrezia sarothrae (six samples) from the Owyhee Mountains of southwestern Idaho. Gas chromatographic analyses (GC-MS, GC-FID, and chiral GC-MS) were carried out on each essential oil sample. The essential oils of A. acanthicarpa were dominated by monoterpene hydrocarbons, including α-pinene (36.7–45.1%), myrcene (21.6–25.5%), and β-phellandrene (4.9–7.0%). Monoterpene hydrocarbons also dominated the essential oils of G. sarothrae, with β-pinene (0.5–18.4%), α-phellandrene (2.2–11.8%), limonene (1.4–25.4%), and (Z)-β-ocimene (18.8–39.4%) as major components. The essential oils of A. ludoviciana showed wide variation in composition, but the relatively abundant compounds were camphor (0.1–61.9%, average 14.1%), 1,8-cineole (0.1–50.8%, average 11.1%), (E)-nerolidol (0.0–41.0%, average 6.8%), and artemisia ketone (0.0–46.1%, average 5.1%). This is the first report on the essential oil composition of A. acanthicarpa and the first report on the enantiomeric distribution in an Ambrosia species. The essential oil compositions of A. ludoviciana and G. sarothrae showed wide variation in composition in this study and compared with previous studies, likely due to subspecies variation.
Protective Effect of Que Zui Tea on d-Galactose-Induced Oxidative Stress Damage in Mice via Regulating SIRT1/Nrf2 Signaling Pathway
Authors: Wang Yongchao , Wang Yongpeng , Zhao Tianrui , Li Mengcheng , Wang Yudan , Cao Jianxin , Liu Yaping , Wang Zhengxuan , Cheng Guiguang
Published: March 2024

Que Zui tea (QT) is an important herbal tea in the diet of the ‘Yi’ people, an ethnic group in China, and it has shown significant antioxidant, anti-inflammatory, and hepatoprotective effects in vitro. This study aims to explore the protective effects of the aqueous-ethanol extract (QE) taken from QT against ᴅ-galactose (ᴅ-gal)-induced oxidative stress damage in mice and its potential mechanisms. QE was identified as UHPLC-HRMS/MS for its chemical composition and possible bioactive substances. Thus, QE is rich in phenolic and flavonoid compounds. Twelve compounds were identified, the main components of which were chlorogenic acid, quinic acid, and 6′-O-caffeoylarbutin. Histopathological and biochemical analysis revealed that QE significantly alleviated brain, liver, and kidney damage in ᴅ-gal-treated mice. Moreover, QE remarkably attenuated oxidative stress by activating the Nrf2/HO-1 pathway to increase the expression of antioxidant indexes, including GSH, GSH-Px, CAT, SOD, and T-AOC. In addition, QE administration could inhibit the IL-1β and IL-6 levels, which suppress the inflammatory response. QE could noticeably alleviate apoptosis by inhibiting the expressions of Caspase-3 and Bax proteins in the brains, livers, and kidneys of mice. The anti-apoptosis mechanism may be related to the upregulation of the SIRT1 protein and the downregulation of the p53 protein induced by QE in the brain, liver, and kidney tissues of mice. Molecular docking analysis demonstrated that the main components of QE, 6′-O-caffeoylarbutin, chlorogenic acid, quinic acid, and robustaside A, had good binding ability with Nrf2 and SIRT1 proteins. The present study indicated that QE could alleviate ᴅ-gal-induced brain, liver and kidney damage in mice by inhibiting the oxidative stress and cell apoptosis; additionally, the potential mechanism may be associated with the SIRT1/Nrf2 signaling pathway.
Phytochemical Evaluation of Terminalia canescens DC. Radlk. Extracts with Antibacterial and Antibiotic Potentiation Activities against Selected β-Lactam Drug-Resistant Bacteria
Authors: Zai Muhammad Jawad , Cheesman Matthew James , Cock Ian Edwin
Published: March 2024

Terminalia canescens DC. Radlk. (family: Combretaceae) is native to northern Australia. Species of the genus Terminalia are widely used as traditional medicines to treat diverse ailments, including bacterial infections. However, we were unable to find any studies that had examined the antimicrobial activity of T. canescens. In this study, T. canescens was screened against a panel of bacterial pathogens, including multi-antibiotic-resistant strains. Solvents with different polarities were used to extract different complements of phytochemicals from T. canescens leaves. Methanolic and aqueous extracts exhibited substantial antimicrobial activity against various pathogens, including those that are multidrug-resistant strains. When combined with some selected clinical antibiotics, some extracts potentiated the antibacterial inhibitory activity. This study identified two synergistic, eleven additive, eleven non-interactive and eight antagonistic interactions. The toxicities of the plant extracts were examined in the Artemia franciscana nauplii assay and were found to be non-toxic, except the aqueous extract, which showed toxicity. Metabolomic liquid chromatography–mass spectrometry (LC-MS) analyses highlighted and identified several flavonoids, including vitexin, quercetin, orientin and kaempferol, as well as the tannins ellagic acid and pyrogallol, which may contribute to the antibacterial activities observed herein. The possible mechanism of action of these extracts was further explored in this study.
Insights into the Synthesis of Spiral Beta Zeolite with Enhanced Catalytic Performance in VOC Abatement
Authors: Bian Chaoqun , Luo Xiaohui , Chen Xiao
Published: March 2024

The rational synthesis of zeolites with designed morphology is a highly challenging task. In this study, we propose 1,5-bis(methylpiperidine)pentylammonium hydroxide (BMPPAOH) as an organic structure-directing agent (OSDA) based on theoretical calculations. The morphology of zeolite samples is characterized by XRD, SEM, TEM, N2 sorption isotherms, and UV Raman spectroscopy. This simple bis-quaternary ammonium salt favored the formation of spiral morphology in Beta zeolite spheres (S-Beta). The crystallization of zeolite in the presence of BMMPAOH is a two-stage process, where nanoparticles agglomerate into spheres in the early stages followed by the emergence of S-Beta crystals with spiral morphology. The synthesized Pt-S-Beta catalysts show higher catalytic activity in VOC abatement compared with other Pt-Beta samples.
Comprehensive Utilization Technology of Aronia melanocarpa
Authors: Shi Dongfang , Xu Jing , Sheng Li , Song Kai
Published: March 2024

Aronia melanocarpa fruit contains a variety of active ingredients, such as phenolic acids, anthocyanins, proanthocyanidins, etc. Relevant in vivo and in vitro studies have concluded that it has beneficial effects in terms of treating dyslipidemia, hypertension, glucose metabolism disorders, etc. This article discusses the nutritional value and food processing of Aronia melanocarpa and reviews the chemical components of Aronia melanocarpa and the pharmacological activities of related substances in order to summarize the chemical characteristics of the fruit and its development prospects. The process optimization of juice production, the impact of antioxidant capacity, and the comprehensive utilization of pomace in feed are discussed. This article provides a reference for future comprehensive application research and product development of Aronia melanocarpa.
Unified Synthesis and Biological Evaluation of Makaluvamine J and Its Analogs
Authors: Kiichi Yo , Fukuoka Koshiro , Kitano Anna , Ishino Koya , Kotoku Naoyuki
Published: March 2024

Makaluvamine J, a pyrroloiminoquinone alkaloid of marine sponge origin, and its analogs were synthesized and assessed for their potential to develop as a novel and selective growth inhibitor targeting human pancreatic cancer PANC-1 cells. Ts-damirone B, a common precursor featuring a pyrroloiminoquinone core structure, was synthesized through Bartoli indole synthesis and IBX-mediated oxidation. Late-stage diversification at N-5 and N-9 yielded makaluvamine J and several analogs. A structure–activity relationship (SAR) analysis highlighted the significance of the lipophilic side chain at N-9 for the growth inhibitory activity of PANC-1 cells. The modest alkyl group at N-5 was found to improve selectivity against other cancer cells. Among the prepared analogs, the tryptamine analog 24 showed potent and selective cytotoxicity (IC50 = 0.029 µM, selective index = 13.1), exceeding those of natural products.
Graphene Materials from Coke-like Wastes as Proactive Support of Nickel–Iron Electro-Catalysts for Water Splitting
Authors: González-Ingelmo María , Rocha Victoria G. , González Zoraida , Sierra Uriel , Barriga Enrique Diaz , Álvarez Patricia
Published: March 2024

Graphene materials, used as electrocatalyst support in green hydrogen production, contribute to increasing the efficiency and robustness of various systems. However, the preparation of a hybrid catalyst containing graphene materials from industrial wastes is still a challenge due to the heterogeneity of the waste. We report the synthesis of 3D electrodes using graphene oxides (GOs) from industrial waste (IW) prepared by immersion onto Toray carbon paper as a 3D support onto GO suspensions and electrodepositing NiFe layered double hydroxides (LDHs). Standard graphite was also used as the reference. The morphology of the two hybrid electrodes was determined by SEM, HRTEM, XPS. Although very similar in both, the sample containing graphene from IW (higher Csp3 hybridization in the graphene layer) has a NiFe phase with less crystallinity and larger presence of Fe2+ ions. These electrodes exhibited similar activity and stability as electrocatalysts of the oxygen evolution reaction (OER), demonstrating the proactive effect of the graphene into the 3D electrode even when this is prepared from heterogeneous industrial waste. Moreover, the defective graphenic structure of the waste GO enhances the reaction kinetics and improves the electron transfer rate, possibly due to the small differences in the electrodeposited NiFe LDH structure.
The Influence of Machining Conditions on the Orientation of Nanocrystallites and Anisotropy of Physical and Mechanical Properties of Flexible Graphite Foils
Authors: Shulyak Vladimir A. , Morozov Nikolai S. , Ivanov Andrei V. , Gracheva Alexandra V. , Chebotarev Sergei N. , Avdeev Viktor V.
Published: March 2024

The physical and mechanical properties and structural condition of flexible graphite foils produced by processing natural graphite with nitric acid, hydrolysis, thermal expansion of graphite and subsequent rolling were studied. The processes of obtaining materials and changing their characteristics has been thoroughly described and demonstrated. The structural transformations of graphite in the manufacture of foils were studied by X-ray diffraction analysis (XRD) and transmission electron microscopy (TEM). A decrease in the average size of the coherent scattering regions (CSR) of nanocrystallites was revealed during the transition from natural graphite to thermally expanded graphite from 57.3 nm to 20.5 nm at a temperature of 900 °C. The rolling pressure ranged from 0.05 MPa to 72.5 MPa. The thickness of the flexible graphite foils varied from 0.11 mm to 0.75 mm, the density—from 0.70 to 1.75 g/cm3. It was shown that with an increase in density within these limits, the compressibility of the graphite foil decreased from 65% to 9%, the recoverability increased from 5% to 60%, and the resiliency decreased from 10% to 6%, which is explained by the structural features of nanocrystallites. The properties’ anisotropy of graphite foils was studied. The tensile strength increased with increasing density from 3.0 MPa (ρ = 0.7 g/cm3) to 14.0 MPa (ρ = 1.75 g/cm3) both in the rolling direction L and across T. At the same time, the anisotropy of physical and mechanical properties increased with an increase in density along L and T to 12% with absolute values of 14.0 MPa against 12.5 MPa at a thickness of 200 μm. Expressed anisotropy was observed along L and T when studying the misorientation angles of nanocrystallites: at ρ = 0.7 g/cm3, it was from 13.4° to 14.4° (up to 5% at the same thickness); at ρ = 1.3 g/cm3—from 11.0° to 12.8° (up to 7%); at ρ = 1.75 g/cm3—from 10.9° to 12.4° (up to 11%). It was found that in graphite foils, there was an increase in the coherent scattering regions in nanocrystallites with an increase in density from 24.8 nm to 49.6 nm. The observed effect can be explained by the coagulation of nanocrystallites by enhancing the Van der Waals interaction between the surface planes of coaxial nanocrystallites, which is accompanied by an increase in microstrains. The results obtained can help discover the mechanism of deformation of porous graphite foils. The obtained results can help discover the deformation mechanism of porous graphite foils. We assume that this will help predict the material behavior under industrial operating conditions of products based flexible graphite foils.