bluuebunny/crossref_metadata_2025 huggingface dataset:
Novel cobalt–carbon@silica adsorbent
Authors: Alotaibi Nusaybah , Hammud Hassan H. , Al Otaibi Nasreen , Hussain Syed Ghazanfar , Prakasam Thirumurugan
Published: October 2020

Recently, carbon nanostructures are of high importance due to their unique characteristics and interesting applications. Pyrolysis of anthracene with cobalt complex Co(2,2′-bipy)Cl2(1), where (2,2′-bipy) is 2,2′-bipyridine, in the absence and presence of silica gave in high yield cobalt-carbon nanocomposite CoCNC (2) and CoCNC@SiO2(3) at 600 °C and 850 °C, respectively. They were characterized using SEM, TEM, PXRD, Raman and XPS. (3) and (2) contain core–shell cobalt(0)/cobalt oxide-graphite with or without silica support. PXRD indicates that (2) contains crystalline hexagonal α-Co and cubic β-Co phases while (3) contains only cubic β-Co phase and silica. The structure of (2) is 3D hierarchical carbon architecture wrapping spherical and elliptical cobalt nanoparticles. (3) consists of graphitized structures around cobalt nanoparticles embedded in the silica matrix. XPS reveals that the nanocomposites contain oxygen functional groups that enhance uptake of cationic dyes. CoCNC@SiO2(3) has higher capacity and thus is better adsorbent of Basic Violet 3 than CoCNC (2). The Langmuir adsorption capacity of (3) is 19.4 mg g−1 while column capacity is 12.55 mg g−1 at 25 °C. Freundlich isotherm and pseudo-second-order kinetic models fit well the adsorption data. Thermodynamics indicate that adsorption(3) is exothermic. Column regeneration was tested for three cycles and Yan et al. was found the best kinetic model.
Seismic signature of the deadly snow avalanche of January 18, 2017, at Rigopiano (Italy)
Authors: Braun Thomas , Frigo Barbara , Chiaia Bernardino , Bartelt Perry , Famiani Daniela , Wassermann Joachim
Published: October 2020

Most snow avalanches occur unobserved, which becomes particularly dramatic when human lives are involved. Seismological observations can be helpful to unravel time and dynamics of unseen events, like the deadly avalanche of January 18, 2017, that hit a Resort-hotel at Rigopiano in the Abruzzi (Italy). Particle motion analysis and spectrograms from data recorded by a close seismic broadband station, calculation of synthetic seismograms, as well as simulation of the flow, allowed us to construct the dynamics of the snow avalanche that buried alive 40 people, killing 29. Due to the bad weather conditions, no visual observation was made, thus making it impossible to determine the exact moment of the avalanche and to report necessary observations of the dramatic event. On-site inspections revealed that the hotel was horizontally cut by shear forces and dislocated by 48 m in 70°N direction, once the increasing avalanche pressure exceeded the structural shear strength of the building. Within an eligible 24 min time range of the avalanche, we found three weak seismic transients, starting at 15:42:38 UTC, recorded by the nearest operating station GIGS located in the Gran Sasso underground laboratory approximately 17 km away. Particle motion analysis of the strongest seismic avalanche signal, as well as of the synthetic seismograms match best when assuming a single force seismic source, attacking in direction of 120°N. Simulation of the avalanche dynamics—calculated by using a 2D rapid mass movement simulator—indicates that the seismic signals were rather generated as the avalanche flowed through a narrow and twisting canyon directly above the hotel. Once the avalanche enters the canyon it is travelling at maximum velocity (37 m/s) and is twice strongly deflected by the rock sidewalls. These impacts created a distinct linearly polarized seismic “avalanche transient”s that can be used to time the destruction of the hotel. Our results demonstrate that seismic recordings combined with simulations of mass movements are indispensable to remotely monitor snow avalanches.
Oxidation of ethidium-based probes by biological radicals: mechanism, kinetics and implications for the detection of superoxide
Authors: Michalski Radosław , Thiebaut David , Michałowski Bartosz , Ayhan Mehmet M. , Hardy Micael , Ouari Olivier , Rostkowski Michał , Smulik-Izydorczyk Renata , Artelska Angelika , Marcinek Andrzej , Zielonka Jacek , Kalyanaraman Balaraman , Sikora Adam
Published: October 2020

Hydroethidine (HE) and hydropropidine ($$\hbox {HPr}^{+}$$

HPr
+

) are fluorogenic probes used for the detection of the intra- and extracellular superoxide radical anion ($$\hbox {O}_{ {2}}^{\bullet -}$$

O

2

∙
-

). In this study, we provide evidence that HE and $$\hbox {HPr}^{+}$$

HPr
+

react rapidly with the biologically relevant radicals, including the hydroxyl radical, peroxyl radicals, the trioxidocarbonate radical anion, nitrogen dioxide, and the glutathionyl radical, via one-electron oxidation, forming the corresponding radical cations. At physiological pH, the radical cations of the probes react rapidly with $$\hbox {O}_{ {2}}^{\bullet -}$$

O

2

∙
-

, leading to the specific 2-hydroxylated cationic products. We determined the rate constants of the reaction between $$\hbox {O}_{ {2}}^{\bullet -}$$

O

2

∙
-

and the radical cations of the probes. We also synthesized N-methylated analogs of $$\hbox {HPr}^{+}$$

HPr
+

and HE which were used in mechanistic studies. Methylation of the amine groups was not found to prevent the reaction between the radical cation of the probe and the superoxide, but it significantly increased the lifetime of the radical cation and had a substantial effect on the profiles of the oxidation products by inhibiting the formation of dimeric products. We conclude that the N-methylated analogs of HE and $$\hbox {HPr}^{+}$$

HPr
+

may be used as a scaffold for the design of a new generation of probes for intra- and extracellular superoxide.
Expansion of US wood pellet industry points to positive trends but the need for continued monitoring
Authors: Aguilar Francisco X. , Mirzaee Ashkan , McGarvey Ronald G. , Shifley Stephen R. , Burtraw Dallas
Published: October 2020

Implementation of the European Union Renewable Energy Directive has triggered exponential growth in trading of pelletized wood fibers. Over 18 million tons of wood pellets were traded by EU member countries in 2018 of which a third were imported from the US. Concerns exist about negative impacts on US forests but systematic assessments are currently lacking. We assessed variability in fundamental attributes for timberland structure and carbon stocks within 123 procurement landscapes of wood pellet mills derived from over 38 thousand forest inventory plots in the eastern US from 2005 to 2017. We found more carbon stocks in live trees, but a fewer number of standing-dead trees, associated with the annual operation of large-scale wood pellet mills. In the US coastal southeast—where US pellet exports to the EU originate—there were fewer live and growing-stock trees and less carbon in soils with every year of milling operation than in the rest of the eastern US—which supplies the domestic market. Greater overlap of mills’ procurement areas exhibited discernible increments across selected carbon stocks. These trends likely reflect more intensive land management practices. Localized forest impacts associated with the wood pellet industry should continue to be monitored.
Snow water scarcity induced by record-breaking warm winter in 2020 in Japan
Authors: Watanabe Satoshi , Kotsuki Shunji , Kanae Shinjiro , Tanaka Kenji , Higuchi Atsushi
Published: October 2020

This study highlights the severity of the low snow water equivalent (SWE) and remarkably high temperatures in 2020 in Japan, where reductions in SWE have significant impacts on society due to its importance for water resources. A continuous 60-year land surface simulation forced by reanalysis data revealed that the low SWE in many river basins in the southern snowy region of mainland Japan are the most severe on record. The impact of the remarkably high temperatures in 2020 on the low SWE was investigated by considering the relationships among SWE, temperature, and precipitation. The main difference between the 2020 case and prior periods of low SWE is the record-breaking high temperatures. Despite the fact that SWE was the lowest in 2020, precipitation was much higher than that in 2019, which was one of the lowest SWE on record pre-2020. The results indicate the possibility that even more serious low-SWE periods will be caused if lower precipitation and higher temperatures occur simultaneously.
A low pKa ligand inhibits cancer-associated pain in mice by activating peripheral mu-opioid receptors
Authors: Baamonde Ana , Menéndez Luis , González-Rodríguez Sara , Lastra Ana , Seitz Viola , Stein Christoph , Machelska Halina
Published: October 2020

The newly designed fentanyl derivative [( ±)-N-(3-fluoro-1-phenethylpiperidine-4-yl)-N-phenyl propionamide] (NFEPP) was recently shown to produce analgesia selectively via peripheral mu-opioid receptors (MOR) at acidic pH in rat inflamed tissues. Here, we examined the pH-dependency of NFEPP binding to brain MOR and its effects on bone cancer-induced pain in mice. The IC50 of NFEPP to displace bound [3H]-DAMGO was significantly higher compared to fentanyl at pH 7.4, but no differences were observed at pH 5.5 or 6.5. Intravenous NFEPP (30–100 nmol/kg) or fentanyl (17–30 nmol/kg) inhibited heat hyperalgesia in mice inoculated with B16-F10 melanoma cells. The peripherally-restricted opioid receptor antagonist naloxone-methiodide reversed the effect of NFEPP (100 nmol/kg), but not of fentanyl (30 nmol/kg). The antihyperalgesic effect of NFEPP was abolished by a selective MOR- (cyprodime), but not delta- (naltrindole) or kappa- (nor-binaltorphimine) receptor antagonists. Ten-fold higher doses of NFEPP than fentanyl induced maximal antinociception in mice without tumors, which was reversed by the non-restricted antagonist naloxone, but not by naloxone-methiodide. NFEPP also reduced heat hyperalgesia produced by fibrosarcoma- (NCTC 2472) or prostate cancer-derived (RM1) cells. These data demonstrate the increased affinity of NFEPP for murine MOR at low pH, and its ability to inhibit bone cancer-induced hyperalgesia through peripheral MOR. In mice, central opioid receptors may be activated by ten-fold higher doses of NFEPP.
A combination of electrochemistry and mass spectrometry to monitor the interaction of reactive species with supported lipid bilayers
Authors: Ravandeh M. , Kahlert H. , Jablonowski H. , Lackmann J.-W. , Striesow J. , Agmo Hernández V. , Wende K.
Published: October 2020

Reactive oxygen and nitrogen species (RONS), e.g. generated by cold physical plasma (CPP) or photodynamic therapy, interfere with redox signaling pathways of mammalian cells, inducing downstream consequences spanning from migratory impairment to apoptotic cell death. However, the more austere impact of RONS on cancer cells remains yet to be clarified. In the present study, a combination of electrochemistry and high-resolution mass spectrometry was developed to investigate the resilience of solid-supported lipid bilayers towards plasma-derived reactive species in dependence of their composition. A 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) lipid bilayer was undisturbed by 200 µM H2O2 (control) but showed full permeability after CPP treatment and space-occupying oxidation products such as PoxnoPC, PAzePC, and POPC hydroperoxide were found. Electron paramagnetic resonance spectroscopy demonstrated the presence of hydroxyl radicals and superoxide anion/hydroperoxyl radicals during the treatment. In contrast, small amounts of the intramembrane antioxidant coenzyme Q10 protected the bilayer to 50% and LysoPC was the only POPC derivative found, confirming the membrane protective effect of Q10. Such, the lipid membrane composition including the presence of antioxidants determines the impact of pro-oxidant signals. Given the differences in membrane composition of cancer and healthy cells, this supports the application of cold physical plasma for cancer treatment. In addition, the developed model using the combination of electrochemistry and mass spectrometry could be a promising method to study the effect of reactive species or mixes thereof generated by chemical or physical sources.
Three-dimensional surface printing method for interconnecting electrodes on opposite sides of substrates
Authors: Rahman Md. Khalilur , Kim Seong-jun , Phung Thanh Huy , Lee Jin-Sol , Yu Jaeryul , Kwon Kye-Si
Published: October 2020

As the application of the direct printing method becomes diversified, printing on substrates with non-flat surfaces is increasingly required. However, printing on three-dimensional surfaces suffers from a number of difficulties, which include ink flow due to gravity, and the connection of print lines over sharp edges. This study presents an effective way to print a fine pattern (~ 30 μm) on three different faces with sharp edge boundaries. The method uses a deflectable and stretchable jet stream of conductive ink, which is produced by near-field electrospinning (NFES) technique. Due to added polymer in the ink, the jet stream from the nozzle is less likely to be disconnected, even when it is deposited over sharp edges of objects. As a practical industrial application, we demonstrate that the method can be effectively used for recent display applications, which require the connection of electrical signal and power on both sides of the glass. When the total length of printed lines along the ‘Π’ shaped glass surfaces was 1.2 mm, we could achieve the average resistance of 0.84 Ω.
Empirical comparison of analytical approaches for identifying molecular HIV-1 clusters
Authors: Novitsky Vlad , Steingrimsson Jon A. , Howison Mark , Gillani Fizza S. , Li Yuanning , Manne Akarsh , Fulton John , Spence Matthew , Parillo Zoanne , Marak Theodore , Chan Philip A. , Bertrand Thomas , Bandy Utpala , Alexander-Scott Nicole , Dunn Casey W. , Hogan Joseph , Kantor Rami
Published: October 2020

Public health interventions guided by clustering of HIV-1 molecular sequences may be impacted by choices of analytical approaches. We identified commonly-used clustering analytical approaches, applied them to 1886 HIV-1 Rhode Island sequences from 2004–2018, and compared concordance in identifying molecular HIV-1 clusters within and between approaches. We used strict (topological support ≥ 0.95; distance 0.015 substitutions/site) and relaxed (topological support 0.80–0.95; distance 0.030–0.045 substitutions/site) thresholds to reflect different epidemiological scenarios. We found that clustering differed by method and threshold and depended more on distance than topological support thresholds. Clustering concordance analyses demonstrated some differences across analytical approaches, with RAxML having the highest (91%) mean summary percent concordance when strict thresholds were applied, and three (RAxML-, FastTree regular bootstrap- and IQ-Tree regular bootstrap-based) analytical approaches having the highest (86%) mean summary percent concordance when relaxed thresholds were applied. We conclude that different analytical approaches can yield diverse HIV-1 clustering outcomes and may need to be differentially used in diverse public health scenarios. Recognizing the variability and limitations of commonly-used methods in cluster identification is important for guiding clustering-triggered interventions to disrupt new transmissions and end the HIV epidemic.
Color image encryption based on chaotic compressed sensing and two-dimensional fractional Fourier transform
Authors: Wang Xingyuan , Su Yining
Published: October 2020

Combining the advantages of structured random measurement matrix and chaotic structure, this paper introduces a color image encryption algorithm based on a structural chaotic measurement matrix and random phase mask. The Chebyshev chaotic sequence is used in the algorithm to generate the flip permutation matrix, the sampling subset and the chaotic cyclic matrix for constructing the structure perceptual matrix and the random phase mask. The original image is compressed and encrypted simultaneously by compressed sensing, and re-encrypted by two-dimensional fractional Fourier transform. Simulation experiments show the effectiveness and reliability of the algorithm.
Successful post-glacial colonization of Europe by single lineage of freshwater amphipod from its Pannonian Plio-Pleistocene diversification hotspot
Authors: Csapó Hedvig , Krzywoźniak Paula , Grabowski Michał , Wattier Remi , Bącela-Spychalska Karolina , Mamos Tomasz , Jelić Mišel , Rewicz Tomasz
Published: October 2020

Gammarus roeseliiGervais, 1835 is a morphospecies with a wide distribution range in Europe. The Balkan Peninsula is known as an area of pre-Pleistocene cryptic diversification within this taxon, resulting in at least 13 Molecular Operational Taxonomic Units (MOTUs). The morphospecies diversified there during Neogene and has probably invaded other parts of the continent very recently, in postglacial or even historical times. Thus, the detailed goals of our study were to (1) identify which lineage(s) colonized Central-Western Europe (CWE), (2) determine their possible geographical origin, (3) verify, whether the colonisation was associated with demographic changes. In total, 663 individuals were sequenced for the cytochrome oxidase I (COI) barcoding fragment and 137 individuals for the internal transcribed spacer II (ITS2). We identified two MOTUs in the study area with contrasting Barcode Index Number and haplotype diversities. The Pannonian Basin (PB) appeared to be a potential ice age refugium for the species, while CWE was colonised by a single lineage (also present in PB), displaying low genetic diversity. Our results suggest thatG. roeseliiis a relatively recent coloniser in CWE, starting demographic expansion around 10 kya.
Molecular imaging analysis of microvesicular and macrovesicular lipid droplets in non-alcoholic fatty liver disease by Raman microscopy
Authors: Minamikawa Takeo , Ichimura-Shimizu Mayuko , Takanari Hiroki , Morimoto Yuki , Shiomi Ryosuke , Tanioka Hiroki , Hase Eiji , Yasui Takeshi , Tsuneyama Koichi
Published: October 2020

Predominant evidence of non-alcoholic fatty liver disease (NAFLD) is the accumulation of excess lipids in the liver. A small group with NAFLD may have a more serious condition named non-alcoholic steatohepatitis (NASH). However, there is a lack of investigation of the accumulated lipids with spatial and molecular information. Raman microscopy has the potential to characterise molecular species and structures of lipids based on molecular vibration and can achieve high spatial resolution at the organelle level. In this study, we aim to demonstrate the feasibility of Raman microscopy for the investigation of NAFLD based on the molecular features of accumulated lipids. By applying the Raman microscopy to the liver of the NASH model mice, we succeeded in visualising the distribution of lipid droplets (LDs) in hepatocytes. The detailed analysis of Raman spectra revealed the difference of molecular structural features of the LDs, such as the degree of saturation of lipids in the LDs. We also found that the inhomogeneous distribution of cholesterol in the LDs depending on the histology of lipid accumulation. We visualised and characterised the lipids of NASH model mice by Raman microscopy at organelle level. Our findings demonstrated that the Raman imaging analysis was feasible to characterise the NAFLD in terms of the molecular species and structures of lipids.
Metabolomic profiles associated with a mouse model of antipsychotic-induced food intake and weight gain
Authors: Zapata Rizaldy C. , Rosenthal Sara Brin , Fisch Kathleen , Dao Khoi , Jain Mohit , Osborn Olivia
Published: October 2020

Antipsychotic drugs (AP) are used to treat a multitude of psychiatric conditions including schizophrenia and bipolar disorder. However, APs also have metabolic side effects including increased food intake and body weight, but the underlying mechanisms remain unknown. We previously reported that minocycline (MINO) co-treatment abrogates olanzapine (OLZ)-induced hyperphagia and weight gain in mice. Using this model, we investigated the changes in the pharmacometabolome in the plasma and hypothalamus associated with OLZ-induced hyperphagia and weight gain. Female C57BL/6 mice were divided into groups and fed either i) control, CON (45% fat diet) ii) CON + MINO, iii) OLZ (45% fat diet with OLZ), iv) OLZ + MINO. We identified one hypothalamic metabolite indoxylsulfuric acid and 389 plasma metabolites (including 19 known metabolites) that were specifically associated with AP-induced hyperphagia and weight gain in mice. We found that plasma citrulline, tricosenoic acid, docosadienoic acid and palmitoleic acid were increased while serine, asparagine and arachidonic acid and its derivatives were decreased in response to OLZ. These changes were specifically blocked by co-treatment with MINO. These pharmacometabolomic profiles associated with AP-induced hyperphagia and weight gain provide candidate biomarkers and mechanistic insights related to the metabolic side effects of these widely used drugs.
Tailored flavoproteins acting as light-driven spin machines pump nuclear hyperpolarization
Authors: Ding Yonghong , Kiryutin Alexey S. , Zhao Ziyue , Xu Qian-Zhao , Zhao Kai-Hong , Kurle Patrick , Bannister Saskia , Kottke Tilman , Sagdeev Renad Z. , Ivanov Konstantin L. , Yurkovskaya Alexandra V. , Matysik Jörg
Published: October 2020

The solid-state photo-chemically induced dynamic nuclear polarization (photo-CIDNP) effect generates non-Boltzmann nuclear spin magnetization, referred to as hyperpolarization, allowing for high gain of sensitivity in nuclear magnetic resonance (NMR). Well known to occur in photosynthetic reaction centers, the effect was also observed in a light-oxygen-voltage (LOV) domain of the blue-light receptor phototropin, in which the functional cysteine was removed to prevent photo-chemical reactions with the cofactor, a flavin mononucleotide (FMN). Upon illumination, the FMN abstracts an electron from a tryptophan to form a transient spin-correlated radical pair (SCRP) generating the photo-CIDNP effect. Here, we report on designed molecular spin-machines producing nuclear hyperpolarization upon illumination: a LOV domain of aureochrome1a from Phaeodactylum tricornutum, and a LOV domain named 4511 from Methylobacterium radiotolerans (Mr4511) which lacks an otherwise conserved tryptophan in its wild-type form. Insertion of the tryptophan at canonical and novel positions in Mr4511 yields photo-CIDNP effects observed by 15N and 1H liquid-state high-resolution NMR with a characteristic magnetic-field dependence indicating an involvement of anisotropic magnetic interactions and a slow-motion regime in the transient paramagnetic state. The heuristic biomimetic design opens new categories of experiments to analyze and apply the photo-CIDNP effect.
Kinetics of SARS-CoV-2 positivity of infected and recovered patients from a single center
Authors: Huang Jia , Zheng Le , Li Zhen , Hao Shiying , Ye Fangfan , Chen Jun , Gans Hayley A. , Yao Xiaoming , Liao Jiayu , Wang Song , Zeng Manfei , Qiu Liping , Li Chunyang , Whitin John C. , Tian Lu , Chubb Henry , Hwa Kuo-Yuan , Ceresnak Scott R. , Zhang Wei , Lu Ying , Maldonado Yvonne A. , McElhinney Doff B. , Sylvester Karl G. , Cohen Harvey J. , Liu Lei , Ling Xuefeng B.
Published: October 2020

Recurrence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) positive detection in infected but recovered individuals has been reported. Patients who have recovered from coronavirus disease 2019 (COVID-19) could profoundly impact the health care system. We sought to define the kinetics and relevance of PCR-positive recurrence during recovery from acute COVID-19 to better understand risks for prolonged infectivity and reinfection. A series of 414 patients with confirmed SARS-Cov-2 infection, at The Second Affiliated Hospital of Southern University of Science and Technology in Shenzhen, China from January 11 to April 23, 2020. Statistical analyses were performed of the clinical, laboratory, radiologic image, medical treatment, and clinical course of admission/quarantine/readmission data, and a recurrence predictive algorithm was developed. 16.7% recovered patients with PCR positive recurring one to three times, despite being in strict quarantine. Younger patients with mild pulmonary respiratory syndrome had higher risk of PCR positivity recurrence. The recurrence prediction model had an area under the ROC curve of 0.786. This case series provides characteristics of patients with recurrent SARS-CoV-2 positivity. Use of a prediction algorithm may identify patients at high risk of recurrent SARS-CoV-2 positivity and help to establish protocols for health policy.
Trimethylamine N-oxide and the reverse cholesterol transport in cardiovascular disease: a cross-sectional study
Authors: Bordoni Laura , Samulak Joanna J. , Sawicka Angelika K. , Pelikant-Malecka Iwona , Radulska Adrianna , Lewicki Lukasz , Kalinowski Leszek , Gabbianelli Rosita , Olek Robert A.
Published: October 2020

The early atherosclerotic lesions develop by the accumulation of arterial foam cells derived mainly from cholesterol-loaded macrophages. Therefore, cholesterol and cholesteryl ester transfer protein (CETP) have been considered as causative in atherosclerosis. Moreover, recent studies indicate the role of trimethylamine N-oxide (TMAO) in development of cardiovascular disease (CVD). The current study aimed to investigate the association between TMAO and CETP polymorphisms (rs12720922 and rs247616), previously identified as a genetic determinant of circulating CETP, in a population of coronary artery disease (CAD) patients (n = 394) and control subjects (n = 153). We also considered age, sex, trimethylamine (TMA) levels and glomerular filtration rate (GFR) as other factors that can potentially play a role in this complex picture. We found no association of TMAO with genetically determined CETP in a population of CAD patients and control subjects. Moreover, we noticed no differences between CAD patients and control subjects in plasma TMAO levels. On the contrary, lower levels of TMA in CAD patients respect to controls were observed. Our results indicated a significant correlation between GFR and TMAO, but not TMA. The debate whether TMAO can be a harmful, diagnostic or protective marker in CVD needs to be continued.
Cost–benefit of limited isolation and testing in COVID-19 mitigation
Authors: Eilersen Andreas , Sneppen Kim
Published: October 2020

The international community has been put in an unprecedented situation by the COVID-19 pandemic. Creating models to describe and quantify alternative mitigation strategies becomes increasingly urgent. In this study, we propose an agent-based model of disease transmission in a society divided into closely connected families, workplaces, and social groups. This allows us to discuss mitigation strategies, including targeted quarantine measures. We find that workplace and more diffuse social contacts are roughly equally important to disease spread, and that an effective lockdown must target both. We examine the cost–benefit of replacing a lockdown with tracing and quarantining contacts of the infected. Quarantine can contribute substantially to mitigation, even if it has short duration and is done within households. When reopening society, testing and quarantining is a strategy that is much cheaper in terms of lost workdays than a long lockdown. A targeted quarantine strategy is quite efficient with only 5 days of quarantine, and its effect increases when testing is more widespread.
Author Correction: Inter-ocular and inter-visit differences in ocular biometry and refractive outcomes after cataract surgery
Authors: Choi Hyun Sup , Yoo Hyo Soon , An Yerim , Yoon Sam Young , Park Sung Pyo , Kim Yong-Kyu
Published: October 2020

An amendment to this paper has been published and can be accessed via a link at the top of the paper.
Accurate detection of cerebellar smooth pursuit eye movement abnormalities via mobile phone video and machine learning
Authors: Chang Zhuoqing , Chen Ziyu , Stephen Christopher D. , Schmahmann Jeremy D. , Wu Hau-Tieng , Sapiro Guillermo , Gupta Anoopum S.
Published: October 2020

Eye movements are disrupted in many neurodegenerative diseases and are frequent and early features in conditions affecting the cerebellum. Characterizing eye movements is important for diagnosis and may be useful for tracking disease progression and response to therapies. Assessments are limited as they require an in-person evaluation by a neurology subspecialist or specialized and expensive equipment. We tested the hypothesis that important eye movement abnormalities in cerebellar disorders (i.e., ataxias) could be captured from iPhone video. Videos of the face were collected from individuals with ataxia (n = 102) and from a comparative population (Parkinson’s disease or healthy participants, n = 61). Computer vision algorithms were used to track the position of the eye which was transformed into high temporal resolution spectral features. Machine learning models trained on eye movement features were able to identify abnormalities in smooth pursuit (a key eye behavior) and accurately distinguish individuals with abnormal pursuit from controls (sensitivity = 0.84, specificity = 0.77). A novel machine learning approach generated severity estimates that correlated well with the clinician scores. We demonstrate the feasibility of capturing eye movement information using an inexpensive and widely accessible technology. This may be a useful approach for disease screening and for measuring severity in clinical trials.
Waist-height ratio and waist are the best estimators of visceral fat in type 1 diabetes
Authors: Parente Erika B. , Mutter Stefan , Harjutsalo Valma , Ahola Aila J. , Forsblom Carol , Groop Per-Henrik
Published: October 2020

Visceral fat is associated with cardiovascular and kidney disease. However, the relationship between body composition and anthropometric measures in type 1 diabetes is unknown. Using z-statistics, we ranked the ability of body mass index (BMI), waist circumference (WC), waist-hip ratio (WHR), waist-height ratio (WHtR) and a body shape index (ABSI) to capture measures of body composition from 603 Dual-energy-X-Ray-Absorptiometry scans of adults with type 1 diabetes. Albuminuria was defined as urinary albumin excretion rate of at least 30 mg/24 h. Women with albuminuria had higher visceral fat mass % (VFM%) (0.9 vs. 0.5%, p = 0.0017) and lower appendicular lean mass % (AppLM%) (25.4 vs 26.4%, p = 0.03) than those without. Men with albuminuria had higher VFM% (1.5 vs. 1.0%, p = 0.0013) and lower AppLM% (30.0 vs 32.3, p < 0.0001) than those without. In men, WHtR estimated VFM% best (z-statistics = 21.1), followed by WC (z = 19.6), BMI (z = 15.1), WHR (z = 14.6) and ABSI (z = 10.1). In women, the ranking was WC (z = 28.9), WHtR (z = 27.3), BMI (z = 20.5), WHR (z = 12.7) and ABSI (z = 10.5). Overall, the ranking was independent of albuminuria. Adults with type 1 diabetes and albuminuria have greater VFM% and lower AppLM% than those without. WHtR and WC best estimate the VFM% in this population, independently of albuminuria and sex.
Transformation of non-water sorbing fly ash to a water sorbing material for drought management
Authors: Saha Abhisekh , Sekharan Sreedeep , Manna Uttam , Sahoo Lingaraj
Published: October 2020

Securing water in the soil through suitable amendments is one of the methods for drought management in arid regions. In this study, a poor water sorbing fly ash was transformed into a high water-absorbing material for improving soil water retention during the drought period. The fly ash water absorbent (FAWA) exhibited high water-absorbing capacity (WAC) of 310 g/g at par with commercially available superabsorbent hydrogel (SAH). The FAWA showed excellent re-swelling behavior for more than eight alternate wetting–drying cycles. The WAC of FAWA was sensitive to salt type, pH, and ionic strength of the solution. At maximum salinity level permitted for plant growth, the WAC of FAWA was 80 g/g indicating its suitability for drought management. There was only a marginal WAC variation in the range of pH (5.5–7.5) considered most suitable for plant growth. The drying characteristics of FAWA amended soil exhibited an increase in desaturation time by 3.3, 2.2, and 1.5 times for fine sand, silt loam, and clay loam, respectively. The study demonstrates the success of using a low rate of FAWA for drought management with the advantage of offering a non-toxic and eco-friendly solution to mass utilization of industrial solid waste for agricultural applications.
Interspecific grafting between Gossypium hirsutum, G. barbadense and G. herbaceum lines
Authors: Karaca Mehmet , Ince Ayse Gul , Reddy Umesh K.
Published: October 2020

Seedling grafting could provide additional crop improvement strategies for cotton. However, there existed limited studies on interspecific grafting and approaches. Four different grafting approaches were developed and compared between lines representing three of the four cultivated cotton species G. hirsutum, G. barbadense and G. herbaceum. Grafting approaches of this study focused on the cotyledon node and cotyledon leaves retained on scions, rootstocks, without cotyledon node and cotyledon leaves on scions and rootstocks or halved cotyledon node and single cotyledon leaf on scions and rootstocks. Evaluations of the grafting approaches were made by comparing survival and growth rate during the second and fifth weeks after transplantation, respectively. The formation of any lateral shoots at the grafted sites were studied in two of four grafting approaches in the first and the second year during flowering stage. DNA alterations due to grafting were investigated using microsatellite markers. There were no statistically significant differences between grafts and their control in survival rate and locus specific DNA alteration. Growth rate and lateral shoot formation, on the other hand, were different among grafting types and grafts. We concluded that grafting without cotyledon node and cotyledon leaves on rootstocks, and with cotyledon node but without cotyledon leaves on scions were easy to perform and suitable for interspecific cotton grafting. Results suggested that grafting seedlings and allowing time to heal graft wounds prior to spring transplanting or double cropping is suitable for wheat–cotton intercropping to prevent late or early chilling damage associated with seed sowing or conventional transplanting of susceptible seedlings. Furthermore, the rapid and consistent wound healing in seedling grafts along with lateral shoot formation occurring in two of four grafting approaches make them a suitable approach to investigate possible genetic and epigenetic movement between scions and rootstocks, especially across species.
Analysis of open chromatin regions in bladder cancer links β-catenin mutations and Wnt signaling with neuronal subtype of bladder cancer
Authors: Eray Aleyna , Güneri Perihan Yağmur , Yılmaz Gülden Özden , Karakülah Gökhan , Erkek-Ozhan Serap
Published: October 2020

Urothelial carcinoma of the bladder is the most frequent bladder cancer affecting more than 400,000 people each year. Histopathologically, it is mainly characterized as muscle invasive bladder cancer (MIBC) and non-muscle invasive bladder cancer (NMIBC). Recently, the studies largely driven by consortiums such as TCGA identified the mutational landscape of both MIBC and NMIBC and determined the molecular subtypes of bladder cancer. Because of the exceptionally high rate of mutations in chromatin proteins, bladder cancer is thought to be a disease of chromatin, pointing out to the importance of studying epigenetic deregulation and the regulatory landscape of this cancer. In this study, we have analyzed ATAC-seq data generated for MIBC and integrated our findings with gene expression and DNA methylation data to identify subgroup specific regulatory patterns for MIBC. Our computational analysis revealed three MIBC regulatory clusters, which we named as neuronal, non-neuronal and luminal outlier. We have identified target genes of neuronal regulatory elements to be involved in WNT signaling, while target genes of non-neuronal and luminal outlier regulatory regions were enriched in epithelial differentiation and drug metabolism, respectively. Neuronal regulatory elements were determined to be ß-catenin targets (p value = 3.59e−08) consisting of genes involved in neurogenesis such as FGF9, and PROX1, and significantly enriched for TCF/LEF binding sites (p value = 1e−584). Our results showed upregulation of ß-catenin targets regulated by neuronal regulatory elements in three different cohorts, implicating ß-catenin signature in neuronal bladder cancer. Further, integration with mutation data revealed significantly higher oncogenic exon 3 ß-catenin mutations in neuronal bladder cancer compared to non-neuronal (odds ratio = 31.33, p value = 1.786e−05). Our results for the first time identify regulatory elements characterizing neuronal bladder cancer and links these neuronal regulatory elements with WNT signaling via mutations in β-catenin and its destruction complex components.
Fine-scale tracking of wild waterfowl and their impact on highly pathogenic avian influenza outbreaks in the Republic of Korea, 2014–2015
Authors: Lee Kyuyoung , Yu Daesung , Martínez-López Beatriz , Yoon Hachung , Kang Sung-Il , Hong Seong-Keun , Lee Ilseob , Kang Yongmyung , Jeong Wooseg , Lee Eunesub
Published: October 2020

Wild migratory waterfowl are considered one of the most important reservoirs and long-distance carriers of highly pathogenic avian influenza (HPAI). Our study aimed to explore the spatial and temporal characteristics of wild migratory waterfowl’s wintering habitat in the Republic of Korea (ROK) and to evaluate the impact of these habitats on the risk of HPAI outbreaks in commercial poultry farms. The habitat use of 344 wild migratory waterfowl over four migration cycles was estimated based on tracking records. The association of habitat use with HPAI H5N8 outbreaks in poultry farms was evaluated using a multilevel logistic regression model. We found that a poultry farm within a wild waterfowl habitat had a 3–8 times higher risk of HPAI outbreak than poultry farms located outside of the habitat. The range of wild waterfowl habitats increased during autumn migration, and was associated with the epidemic peak of HPAI outbreaks on domestic poultry farms in the ROK. Our findings provide a better understanding of the dynamics of HPAI infection in the wildlife–domestic poultry interface and may help to establish early detection, and cost-effective preventive measures.
Broadband high-resolution molecular spectroscopy with interleaved mid-infrared frequency combs
Authors: Muraviev A. V. , Konnov D. , Vodopyanov K. L.
Published: October 2020

Traditionally, there has been a trade-off in spectroscopic measurements between high resolution, broadband coverage, and acquisition time. Originally envisioned for precision spectroscopy of the hydrogen atom in the ultraviolet, optical frequency combs are now commonly used for probing molecular ro-vibrational transitions throughout broad spectral bands in the mid-infrared providing superior resolution, speed, and the capability of referencing to the primary frequency standards. Here we demonstrate the acquisition of 2.5 million spectral data points over the continuous wavelength range of 3.17–5.13 µm (frequency span 1200 cm−1, sampling point spacing 13–21 MHz), via interleaving comb-tooth-resolved spectra acquired with a highly-coherent broadband dual-frequency-comb system based on optical subharmonic generation. With the original comb-line spacing of 115 MHz, overlaying eight spectra with gradually shifted comb lines we fully resolve the amplitude and phase spectra of molecules with narrow Doppler lines, such as carbon disulfide (CS2) and its three isotopologues.
Genetical control of 2D pattern and depth of the primordial furrow that prefigures 3D shape of the rhinoceros beetle horn
Authors: Adachi Haruhiko , Matsuda Keisuke , Niimi Teruyuki , Kondo Shigeru , Gotoh Hiroki
Published: October 2020

The head horn of the Asian rhinoceros beetle develops as an extensively folded primordium before unfurling into its final 3D shape at the pupal molt. The information of the final 3D structure of the beetle horn is prefigured in the folding pattern of the developing primordium. However, the developmental mechanism underlying epithelial folding of the primordium is unknown. In this study, we addressed this gap in our understanding of the developmental patterning of the 3D horn shape of beetles by focusing on the formation of furrows at the surface of the primordium that become the bifurcated 3D shape of the horn. By gene knockdown analysis via RNAi, we found that knockdown of the gene Notch disturbed overall horn primordial furrow depth without affecting the 2D furrow pattern. In contrast, knockdown of CyclinE altered 2D horn primordial furrow pattern without affecting furrow depth. Our results show how the depth and 2D pattern of primordial surface furrows are regulated at least partially independently during beetle horn development, and how both can alter the final 3D shape of the horn.
Positive attitudes and negative expectations in lonely individuals
Authors: Bellucci Gabriele
Published: October 2020

Loneliness is a central predictor of depression and major factor of all-cause mortality. Loneliness is supposed to be a warning signal prompting individuals to seek out social connections. However, lonely individuals seem to be less likely to engage in prosocial activities and are overall more socially withdrawn. Hence, it is yet unclear whether and how loneliness affects an individual’s social motivations. Prosocial attitudes and expectations about social interactions of lonely individuals might shed light on whether lonely individuals are more prone to connect or withdraw from social activities. Here, results from a large dataset (~ 15,500 individuals) provide evidence for both. In particular, lonely individuals indicate stronger altruistic attitudes, suggesting a positive tendency to build and maintain social bonds. However, they also report more negative expectations about others, as they believe their social partners be less fair and trustworthy, suggesting less favorable evaluations of social interactions. By highlighting an important link between loneliness, prosocial attitudes and social expectations, this work stresses the role of loneliness in social motivations, points to potential consequences for social behaviors, and proposes a mechanism for the paradoxical effects of loneliness on an individual’s social attitudes and expectations, with important implications for future basic and clinical research, as well as education, economics and public policy.
Contralateral spreading of substances following intratympanic nanoparticle-conjugated gentamicin injection in a rat model
Authors: Lee Sang-Yeon , Kim Jeonghyo , Oh Sangjin , Jung Gaon , Jeong Ki-Jae , Tan Tran Van , Hwang Dajeong , Kim SungIl , Song Jae-Jin , Suh Myung-Whan , Lee Jaebeom , Koo Ja-Won
Published: October 2020

This study was performed to investigate the Eustachian tube as a potential route for contralateral spreading following intratympanic nanoparticle (NP)-conjugated gentamicin injection in a rat model. Sprague–Dawley rats were divided into three groups and substances were injected in the right ear: group 1 (fluorescent magnetic nanoparticles [F-MNPs], n = 4), group 2 (F-MNP-conjugated gentamicin [F-MNP@GM], n = 2), and control group (no injections, n = 2). T2-weighted sequences corresponding to the regions of interest at 1, 2, and 3 h after intratympanic injection were evaluated, along with immunostaining fluorescence of both side cochlea. The heterogeneous signal intensity of F-MNPs and F-MNP@GM on T2-weighted images, observed in the ipsilateral tympanum, was also detected in the contralateral tympanum in 4 out of 6 rats, recapitulating fluorescent nanoparticles in the contralateral cochlear hair cells. Computational simulations demonstrate the contralateral spreading of particles by gravity force following intratympanic injection in a rat model. The diffusion rate of the contralateral spreading relies on the sizes and surface charges of particles. Collectively, the Eustachian tube could be a route for contralateral spreading following intratympanic injection. Caution should be taken when using the contralateral ear as a control study investigating inner-ear drug delivery through the transtympanic approach.
Computational modelling of patient specific spring assisted lambdoid craniosynostosis correction
Authors: Bozkurt Selim , Borghi Alessandro , van de Lande Lara S. , Jeelani N. U. Owase , Dunaway David J. , Schievano Silvia
Published: October 2020

Lambdoid craniosynostosis (LC) is a rare non-syndromic craniosynostosis characterised by fusion of the lambdoid sutures at the back of the head. Surgical correction including the spring assisted cranioplasty is the only option to correct the asymmetry at the skull in LC. However, the aesthetic outcome from spring assisted cranioplasty may remain suboptimal. The aim of this study is to develop a parametric finite element (FE) model of the LC skulls that could be used in the future to optimise spring surgery. The skull geometries from three different LC patients who underwent spring correction were reconstructed from the pre-operative computed tomography (CT) in Simpleware ScanIP. Initially, the skull growth between the pre-operative CT imaging and surgical intervention was simulated using MSC Marc. The osteotomies and spring implantation were performed to simulate the skull expansion due to the spring forces and skull growth between surgery and post-operative CT imaging in MSC Marc. Surface deviation between the FE models and post-operative skull models reconstructed from CT images changed between ± 5 mm over the skull geometries. Replicating spring assisted cranioplasty in LC patients allow to tune the parameters for surgical planning, which may help to improve outcomes in LC surgeries in the future.
Delineating colorectal cancer distribution, interaction, and risk prediction by environmental risk factors and serum trace elements
Authors: Nawi Azmawati Mohammed , Chin Siok Fong , Mazlan Luqman , Jamal Rahman
Published: October 2020

The burden of colorectal cancer (CRC) is increasing worldwide especially in developing countries. This phenomenon may be attributable to lifestyle, dietary and environmental risk factors. We aimed to determine the level of 25 trace elements, their interaction with environmental risk factors, and subsequently develop a risk prediction model for CRC (RPM CRC). For the discovery phase, we used a hospital-based case–control study (CRC and non-CRC patients) and in the validation phase we analysed pre-symptomatic samples of CRC patients from The Malaysian Cohort Biobank. Information on the environmental risk factors were obtained and level of 25 trace elements measured using the ICP-MS method. CRC patients had lower Zn and Se levels but higher Li, Be, Al, Co, Cu, As, Cd, Rb, Ba, Hg, Tl, and Pb levels compared to non-CRC patients. The positive interaction between red meat intake ≥ 50 g/day and Co ≥ 4.77 µg/L (AP 0.97; 95% CI 0.91, 1.03) doubled the risk of CRC. A panel of 24 trace elements can predict simultaneously and accurate of high, moderate, and low risk of CRC (accuracy 100%, AUC 1.00). This study provides a new input on possible roles for various trace elements in CRC as well as using a panel of trace elements as a screening approach to CRC.