bluuebunny/crossref_metadata_2025 huggingface dataset:
The Effects of Sensory Threshold Somatosensory Electrical Stimulation on Users With Different MI-BCI Performance
Authors: Chen Long , Zhang Lei , Wang Zhongpeng , Gu Bin , Zhang Xin , Ming Dong
Published: June 2022

Motor imagery-based brain-computer interface (MI-BCI) has been largely studied to improve motor learning and promote motor recovery. However, the difficulty in performing MI limits the widespread application of MI-BCI. It has been suggested that the usage of sensory threshold somatosensory electrical stimulation (st-SES) is a promising way to guide participants on MI tasks, but it is still unclear whether st-SES is effective for all users. In the present study, we aimed to examine the effects of st-SES on the MI-BCI performance in two BCI groups (High Performers and Low Performers). Twenty healthy participants were recruited to perform MI and resting tasks with EEG recordings. These tasks were modulated with or without st-SES. We demonstrated that st-SES improved the performance of MI-BCI in the Low Performers, but led to a decrease in the accuracy of MI-BCI in the High Performers. Furthermore, for the Low Performers, the combination of st-SES and MI resulted in significantly greater event-related desynchronization (ERD) and sample entropy of sensorimotor rhythm than MI alone. However, the ERD and sample entropy values of MI did not change significantly during the st-SES intervention in the High Performers. Moreover, we found that st-SES had an effect on the functional connectivity of the fronto-parietal network in the alpha band of Low Performers and the beta band of High Performers, respectively. Our results demonstrated that somatosensory input based on st-SES was only beneficial for sensorimotor cortical activation and MI-BCI performance in the Low Performers, but not in the High Performers. These findings help to optimize guidance strategies to adapt to different categories of users in the practical application of MI-BCI.
The Impaired Subcortical Pathway From Superior Colliculus to the Amygdala in Boys With Autism Spectrum Disorder
Authors: Huang Yiting , Vangel Mark , Chen Helen , Eshel Maya , Cheng Ming , Lu Tao , Kong Jian
Published: June 2022

Objective

Increasing evidence suggests that a subcortical pathway from the superior colliculus (SC) through the pulvinar to the amygdala plays a crucial role in mediating non-conscious processing in response to emotional visual stimuli. Given the atypical eye gaze and response patterns to visual affective stimuli in autism, we examined the functional and white matter structural difference of the pathway in boys with autism spectrum disorder (ASD) and typically developing (TD) boys.

Methods

A total of 38 boys with ASD and 38 TD boys were included. We reconstructed the SC-pulvinar-amygdala pathway in boys with ASD and TD using tractography and analyzed tract-specific measurements to compare the white matter difference between the two groups. A region of interest-based functional analysis was also applied among the key nodes of the pathway to explore the functional connectivity network.

Results

Diffusion tensor imaging analysis showed decreased fractional anisotropy (FA) in pathways for boys with ASD compared to TD. The FA change was significantly associated with the atypical communication pattern in boys with ASD. In addition, compared to TD, we found that the ASD group was associated with increased functional connectivity between the right pulvinar and the left SC.

Conclusion

Our results indicated that the functional and white matter microstructure of the subcortical route to the amygdala might be altered in individuals with autism. This atypical structural change of the SC-pulvinar-amygdala pathway may be related to the abnormal communication patterns in boys with ASD.
Molecular Determinants of Mechanical Itch Sensitization in Chronic Itch
Authors: Lee Hankyu , Graham Robert D. , Melikyan Diana , Smith Brennan , Mirzakhalili Ehsan , Lempka Scott F. , Duan Bo
Published: June 2022

Chronic itch is associated with sensitization of the somatosensory nervous system. Recent studies have identified the neural circuits transmitting acute itch; however, the mechanisms by which itch transforms into a pathological state remain largely unknown. We have previously shown that Aβ low-threshold mechanoreceptors, together with spinal urocortin 3-positive (Ucn3+) excitatory interneurons and neuropeptide Y-positive (NPY+) inhibitory interneurons, form a microcircuit that transmits and gates acute mechanical itch. Here, using whole-cell patch-clamp recordings, we observed increased excitability in spinal Ucn3+ neurons under chronic itch conditions. In contrast to Ucn3+ neurons, the excitability of spinal NPY+ neurons was largely reduced under chronic itch conditions. To explore the molecular mechanisms underlying sensitization of this microcircuit, we examined the mRNA expression levels of voltage-gated ion channels in recorded spinal Ucn3+ and NPY+ neurons by single-cell quantitative real-time PCR (qRT-PCR). We found that the expression levels of Nav1.6 and Cav2.3 channels were increased in spinal Ucn3+ neurons in chronic itch mice, while the expression level of SK3 channels was decreased. By contrast, the expression levels of Nav1.6 and BK channels were decreased in spinal NPY+ neurons in chronic itch mice. To determine the contribution of different ion channels in chronic itch sensitization, we then used a Markov Chain Monte Carlo method to parameterize a large number of biophysically distinct multicompartment models of Ucn3+ and NPY+ neurons. These models included explicit representations of the ion channels that we found to be up- or down-regulated under chronic itch conditions. Our models demonstrated that changes in Nav1.6 conductance are predominantly responsible for the changes in excitability of both Ucn3+ and NPY+ neurons during chronic itch pathogenesis. Furthermore, when simulating microcircuits of our Ucn3+ and NPY+ models, we found that reduced Nav1.6 conductance in NPY+ models played a major role in opening the itch gate under chronic itch conditions. However, changing SK, BK, or R-type calcium channel conductance had negligible effects on the sensitization of this circuit. Therefore, our results suggest that Nav1.6 channels may play an essential role in mechanical itch sensitization. The findings presented here may open a new avenue for developing pharmaceutical strategies to treat chronic itch.
Protein and Sport: Alternative Sources and Strategies for Bioactive and Sustainable Sports Nutrition
Authors: López-Martínez Manuel I. , Miguel Marta , Garcés-Rimón Marta
Published: June 2022

Nutrition and sport play an important role in achieving a healthy lifestyle. In addition to the intake of nutrients derived from the normal diet, some sport disciplines require the consumption of supplements that contribute positively to improved athletic performance. Protein intake is important for many aspects related to health, and current evidence suggests that some athletes require increased amounts of this nutrient. On the other hand, society's demand for more environmentally friendly products, focus on the search for alternative food sources more sustainable. This review aims to summarize the latest research on novel strategies and sources for greener and functional supplementation in sport nutrition. Alternative protein sources such as insects, plants or mycoproteins have proven to be an interesting substrate due to their high added value in terms of bioactivity and sustainability. Protein hydrolysis has proven to be a very useful technology to revalue by-products, such as collagen, by producing bioactive peptides beneficial on athletes performance and sport-related complications. In addition, it has been observed that certain amino acids from plant sources, as citrulline or theanine, can have an ergogenic effect for this target population. Finally, the future perspectives of protein supplementation in sports nutrition are discussed. In summary, protein supplementation in sports nutrition is a very promising field of research, whose future perspective lies with the search for alternatives with greater bioactive potential and more sustainable than conventional sources.
Microbubbles Ultrasonic Cavitation Regulates Tumor Interstitial Fluid Pressure and Enhances Sonodynamic Therapy
Authors: Xi Fen , Feng Yuyi , Chen Qiaoli , Chen Liping , Liu Jianhua
Published: June 2022

Sonodynamic therapy (SDT) is a promising treatment method for solid tumors. However, the high interstitial fluid pressure (IFP) in tumor tissues limits the accumulation of sonosensitizers. In the present study, microbubbles ultrasonic cavitation was used to regulate the tumor’s IFP and evaluate SDT effects. Rabbit VX2 tumor tissues were treated with microbubbles ultrasonic cavitation. The IFP of different tumor parts before and after cavitation was measured by the WIN method. The accumulation of the sonosensitizers hematoporphyrin monomethyl ether (HMME) in tumor tissues was observed using an ultramicro spectrophotometer and laser confocal microscope. Then, tumor-bearing rabbits were treated with SDT once a week for eight weeks and the therapeutic effect was evaluated. After microbubbles ultrasonic cavitation treatment, the tumor’s IFP decreased and the HMME concentration increased. We concluded that microbubbles ultrasonic cavitation can increase HMME accumulation in rabbit VX2 tumors and increase SDT therapeutic effects.
Adjuvant Treatments of Adult Melanoma: A Systematic Review and Network Meta-Analysis
Authors: Jing Mingyi , Cai Yi , Shi Jing , Zhang Xufan , Zhu Baohua , Yuan Fan , Zhang Jie , Xiao Min , Chen Mingling
Published: June 2022

Multiple treatments of unresectable advanced or metastatic melanoma have been licensed in the adjuvant setting, causing tremendous interest in developing neoadjuvant strategies for melanoma. Eligible studies included those that compared overall survival/progression-free survival/grade 3 or 4 adverse events in patients with unresectable advanced or metastatic melanoma. Seven eligible randomized trials with nine publications were included in this study. Direct and network meta-analysis consistently indicated that nivolumab+ipilimumab, nivolumab, and trametinib could significantly improve overall survival and progression-free survival compared to ipilimumab in advanced melanoma patients. Compared to ipilimumab, nivolumab, dacarbazine, and ipilimumab+gp100 had a reduced risk of grade 3/4 adverse reactions. The nivolumab+ipilimumab combination had the highest risk of adverse events, followed by ipilimumab+dacarbazine and trametinib. Combination therapy was more beneficial to improve overall survival and progression-free survival than monotherapy in advanced melanoma treatment, albeit at the cost of increased toxicity. Regarding the overall survival/progression-free survival, ipilimumab+gp100 ranked below ipilimumab+dacarbazine and nivolumab+ipilimumab, although it had a smaller rate of grade 3 or 4 AEs than other treatments (except nivolumab). Nivolumab is the optimum adjuvant treatment for unresectable advanced or metastatic melanoma with a good risk-benefit profile. In order to choose the best therapy, clinicians must consider the efficacy, adverse events, and physical status.
Small Airway Dysfunction Measured by Impulse Oscillometry and Fractional Exhaled Nitric Oxide Is Associated With Asthma Control in Children
Authors: Lin Liang-Mei , Chang Yu-Jun , Yang Kuender D. , Lin Ching-Hsiung , Chien Jien-Wen , Kao Jun-Kai , Lee Ming-Sheng , Chiang Tsay-I , Lin Ching-Yuang , Tsai Yi-Giien
Published: June 2022

Background

Impulse oscillometry (IOS) and fractional exhaled nitric oxide (FeNO) are sensitive and non-invasive methods to measure airway resistance and inflammation, although there are limited population-based studies using IOS and FeNO to predict asthma control.

Objective

This study aimed to investigate the utility of IOS and FeNO for assessing childhood asthma control in terms of small airway dysfunction and airway inflammation.

Methods

This prospective observational cohort study enrolled 5,018 school children (aged 6–12 years), including 560 asthmatic children and 140 normal participants. FeNO, spirometry, IOS, bronchial dilation test, total IgE, and childhood asthma control test (C-ACT) were measured. FeNO, IOS, spirometry, and C-ACT results were correlated with childhood asthma with and without control.

Results

Uncontrolled asthmatic children had abnormal FeNO, IOS, and spirometric values compared with control subjects (P < 0.05). IOS parameters with R5, R5-R20, X5, Ax, △R5, and FeNO can predict lower C-ACT scales by the areas under receiver operating characteristic curves (AUCs) (0.616, 0.625, 0.609, 0.622, 0.625, and 0.714). A combination of FeNO (>20 ppb) with IOS measure significantly increased the specificity for predicting uncontrolled asthma patients compared with FeNO alone (P < 0.01). A multiple regression model showed that small airway parameter (R5-R20) was the strongest risk factor [OR (95% CI): 87.26 (7.67–993.31)] for uncontrolled asthma patients. Poor control with lower C-ACT scales correlated with high FeNO (r = −0.394), R5 (r = −0.106), and R5-R20 (r = −0.129) in asthmatic children (P < 0.05).

Conclusion

A combined use of FeNO and IOS measurements strongly predicts childhood asthma with or without control.
Case Report: Primary Thoracic Low-Grade Fibromyxoid Sarcoma in a Young Girl Presenting With Mediastinal Mass Syndrome
Authors: Chan Yat Chi , Kan Amanda N. C. , Yuen Liz Y. P. , Wan Innes Y. P. , Fung Kevin K. F. , Cheung Yiu-fai , Leung Karen K. Y. , Ku Dennis T. L. , Liu Anthony P. Y.
Published: June 2022

Low-grade fibromyxoid sarcomas (LGFMSs) are typically adult-onset tumors that arise from the extremities. Here, we report an exceptional case of primary thoracic LGFMS in an 8-year-old girl that resulted in mediastinal syndrome. In reporting this case, we discuss the clinical challenges, role of molecular profiling and review reported cases of pediatric thoracic LGFMSs.
Risk Factors for Physical Function Impairments in Postintensive Care Syndrome: A Scoping Review
Authors: Ding Min , Yang Chunfeng , Li Yumei
Published: June 2022

Objective

Survivors of critical illness may experience short- and long-term physical function impairments. This review aimed to identify the risk factors for physical function impairments from the current literature.

Data Sources

A systematic search of the PubMed, Embase, Web of Science, and Cochrane Library databases following the Preferred Reporting Items for Systematic Reviews and Meta-analyses extension for Scoping Reviews guideline was performed.

Study Selection

The risk factors reported in all human studies reporting physical function impairments in children admitted to the pediatric intensive care unit (PICU) were reviewed and categorized. Two investigators independently screened, evaluated, and selected studies for inclusion. Data from eligible studies were extracted by one investigator, and another investigator reviewed and verified the data. A systematic narrative approach was employed to review and summarize the data.

Results

A total of 264 studies were found to be eligible, with 19 studies meeting the inclusion criteria. Children admitted to the PICU experienced physical function impairments during their stay, which can last for years. The studies varied primarily in the measurement timing and tools used. The most frequently reported risk factors for physical function impairments were age, race or ethnicity, a pre-admission chronic condition, sex, disease severity, duration or the presence of mechanical ventilation, and admission diagnosis.

Conclusions

Physical function impairments may be persistent in PICU survivors. To prevent these impairments in critically ill patients, pediatricians should pay attention to modifiable risk factors, such as the duration of mechanical ventilation. Future studies need to promote a combination of standardized measures for the detection and prevention of physical function impairments.
Case Report: Glycogen Storage Disease Type Ia in a Chinese Child Treated With Growth Hormone
Authors: Wu Shimin , Guo Shusen , Fu Lina , Du Caiqi , Luo Xiaoping
Published: June 2022

Background

Glycogen storage disease type Ia is a rare metabolic disorder that leads to excessive glycogen and fat accumulation in organs, characterized by hepatomegaly, hypoglycemia, lactic acidemia, hyperlipidemia, hyperuricemia, puberty delay, and growth retardation. Here, we report on a patient with glycogen storage disease type Ia treated with growth hormone.

Case Presentation

A 10-year-old boy had growth retardation for 6 years, and was admitted to clarify the cause of his short stature. We found that his bone age was 5.5 years, significantly lower than his physical age, while his serum IGF-1 and IGFBP-3 were 23.30 and 1620.0 ng/mL, respectively, both lower than normal. His medical history revealed that he had suffered from steatohepatitis, hyperlipidemia, and hypoglycemia since he was 11 months of age. Whole exome sequencing (WES) showed compound heterozygous mutations in exons 2 and 5 of the glucose-6-phosphatase (G6PC) gene on chromosome 17: c.G248A (p.R83H) and c.G648T (p.L216L). The patient was finally diagnosed with GSD Ia. After growth hormone (GH) treatment and corn starch therapy for 14 months, his height significantly increased (by 13 cm). The serum IGF-1 level increased to the normal range but his lipid levels and liver function did not significantly increase.

Conclusion

We describe a young patient with a compound heterozygous G6PC variant in a Chinese family; his height increased significantly after growth hormone and corn starch interventions. This case emphasizes that WES is essential for early diagnosis, and that growth hormone treatment may increase the height of patients with GSD Ia safely.
Target Specific Inhibition of Protein Tyrosine Kinase in Conjunction With Cancer and SARS-COV-2 by Olive Nutraceuticals
Authors: Ghosh Arabinda , Mukerjee Nobendu , Sharma Bhavdeep , Pant Anushree , Kishore Mohanta Yugal , Jawarkar Rahul D. , Bakal Ravindrakumar L. , Terefe Ermias Mergia , Batiha Gaber El-Saber , Mostafa-Hedeab Gomaa , Aref Albezrah Nisreen Khalid , Dey Abhijit , Baishya Debabrat
Published: June 2022

The fact that viruses cause human cancer dates back to the early 1980s. By reprogramming cellular signaling pathways, viruses encoded protein that can regulate altered control of cell cycle events. Viruses can interact with a superfamily of membrane bound protein, receptor tyrosine kinase to modulate their activity in order to increase virus entrance into cells and promotion of viral replication within the host. Therefore, our study aimed at screening of inhibitors of tyrosine kinase using natural compounds from olive. Protein tyrosine kinase (PTK) is an important factor for cancer progression and can be linked to coronavirus. It is evident that over expression of Protein tyrosine kinase (PTK) enhance viral endocytosis and proliferation and the use of tyrosine kinase inhibitors reduced the period of infection period. Functional network studies were carried out using two major PTKs viz. Anaplastic lymphoma kinase (ALK) and B-lymphocytic kinase (BTK). They are associated with coronavirus in regulation of cell signaling proteins for cellular processes. We virtually screened for 161 library of natural compounds from olive found overexpressed in ALK and BTK in metastatic as well as virus host cells. We have employed both ligand and target-based approach for drug designing by high throughput screening using Multilinear regression model based QSAR and docking. The QSAR based virtual screening of 161 olive nutraceutical compounds has successfully identified certain new hit; Wedelosin, in which, the descriptor rsa (ratio of molecular surface area to the solvent accessible surface area) plays crucial role in deciding Wedelosin’s inhibitory potency. The best-docked olive nutraceuticals further investigated for the stability and effectivity of the BTK and ALK during in 150 ns molecular dynamics and simulation. Post simulation analysis and binding energy estimation in MMGBSA further revealed the intensive potential of the olive nutraceuticals in PTK inhibition. This study is therefore expected to widen the use of nutraceuticals from olive in cancer as well as SARS-CoV2 alternative therapy.
Investigational Drugs for the Treatment of Depression (Part 2): Glutamatergic, Cholinergic, Sestrin Modulators, and Other Agents
Authors: Vasiliu Octavian
Published: June 2022

Many investigational drugs with antidepressant activity are currently explored in different phases of clinical research, with indications such as major depressive disorder, treatment-resistant major depression, bipolar depression, post-partum depression, and late-life depression. Although the vast majority of the antidepressants in clinical use are based on the monoaminergic hypothesis of depression, recent data supported the launching on the market of two new, non-monoamine-modulating drugs. Esketamine for treatment-resistant major depression and brexanolone for post-partum depression are two exceptions from the monoaminergic model, although their use is still limited by high costs, unique way of administration (only intravenously for brexanolone), physicians’ reluctance to prescribe new drugs, and patients’ reticence to use them. Glutamatergic neurotransmission is explored based on the positive results obtained by intranasal esketamine, with subanesthetic intravenous doses of ketamine, and D-cycloserine, traxoprodil, MK-0657, AXS-05, AVP-786, combinations of cycloserine and lurasidone, or dextromethorphan and quinidine, explored as therapeutic options for mono- or bipolar depression. Sestrin modulators, cholinergic receptor modulators, or onabotulinumtoxinA have also been investigated for potential antidepressant activity. In conclusion, there is hope for new treatments in uni- and bipolar depression, as it became clear, after almost 7 decades of monoamine-modulating antidepressants, that new pathogenetic pathways should be targeted to increase the response rate in this population.
Identification of Linomide Derivatives as Potential Anticancer Therapeutics using Molecular Docking Studies
Authors: Borges e Soares Giselle A. , Bhattacharya Tanima , Mamledesai Shivalingrao , Ai Zhaoquan , Hasan Alexandru Madalin , Cavalu Simona
Published: June 2022

12 analogs bearing a structural similarity to Linomide, a bonafide anticancer agent were synthesized wherein cyclization of substituted dianilides rendered 4-hydroxyquinolin-2(1H)-ones that were subjected to a Mannich reaction to yield 4-hydroxy-3-(substituted-1-ylmethyl) quinolin-2(1H)-one analogs. Characterization was performed using IR, 1H nuclear magnetic resonance and 13C NMR spectral analysis. Subsequently, in vitro anticancer studies revealed that Compound 4b showed maximum cytotoxicity with IC50 values of 1.539 μM/ml and 1.732 μM/ml against A549 and K562 cell lines respectively. This, however, is lower in comparison with standard Paclitaxel (IC50 values of 0.3 μM/ml for both cell lines). Surprisingly, docking studies at the active site of EGFRK revealed Compound 4b possessed a MolDock Score of -110.2253 that is highly comparable to the standard 4-anilinoquinazoline (MolDock Score of -112.04). Our computational and biological data thus provides an insight on the cytotoxicity of these derivatives and warrants future research that can possibly lead to the development of potent anticancer therapeutics.
The Correlation Between Busulfan Exposure and Clinical Outcomes in Chinese Pediatric Patients: A Population Pharmacokinetic Study
Authors: Du Xiaohuan , Huang Chenrong , Xue Ling , Jiao Zheng , Zhu Min , Li Jie , Lu Jun , Xiao Peifang , Zhou Xuemei , Mao Chenmei , Zhu Zengyan , Dong Ji , Liu Xiaoxue , Chen Zhiyao , Zhang Shichao , Ding Yiduo , Hu Shaoyan , Miao Liyan
Published: June 2022

Aims: The aims of the study were to 1) establish a population pharmacokinetic (Pop-PK) model for busulfan in Chinese pediatric patients undergoing hematopoietic stem cell transplantation (HSCT) and then estimate busulfan exposure and 2) explore the association between busulfan exposure and clinical outcomes.

Methods: A total of 128 patients with 467 busulfan concentrations were obtained for Pop-PK modeling using nonlinear mixed effect model (NONMEM) software. Sixty-three patients who received the 16-dose busulfan conditioning regimen were enrolled to explore the correlations between clinical outcomes and the busulfan area under the concentration–time curve (AUC) using the Cox proportional hazards regression model, Kaplan–Meier method and logistic regression.

Results: The typical values for clearance (CL) and distribution volume (V) of busulfan were 7.71 L h−1 and 42.4 L, respectively. The allometric normal fat mass (NFM) and maturation function (Fmat) can be used to describe the variability in CL, and the fat-free mass (FFM) can be used to describe the variability in V. Patients with AUCs of 950–1,600 µM × min had 83.7% (95% CI: 73.3–95.5) event-free survival (EFS) compared with 55.0% (95% CI: 37.0–81.8) for patients with low or high exposure (p = 0.024). The logistic regression analysis results showed no association between transplant-related toxicities and the busulfan AUC (p > 0.05).

Conclusions: The variability in busulfan CL was related to the NFM and Fmat, while busulfan V was related to the FFM. Preliminary analysis results suggested that a busulfan AUC of 950–1,600 µM × min was associated with better EFS in children receiving the 16-dose busulfan regimen.
Berberine Suppresses Lung Metastasis of Cancer via Inhibiting Endothelial Transforming Growth Factor Beta Receptor 1
Authors: Tian Wenjia , Hao Huifeng , Chu Ming , Gong Jingjing , Li Wenzhe , Fang Yuan , Zhang Jindong , Zhang Cunzheng , Huang Yonghui , Pei Fei , Duan Liping
Published: June 2022

This study investigated the effects of berberine (BBR) on pancreatic cancer (PC) lung metastasis and explored the underlying mechanisms, using a BALB/C-nu/nu nude mouse model injected with PC cells (AsPC-1). Intragastric administration of BBR dose-dependently improves survival of mice intravenously injected with AsPC-1 cells, and reduces lung metastasis. Especially, BBR significantly reduces lung infiltration of circulating tumor cells (CTCs) 24 h after AsPC-1 cells injection. In vitro, tumor cells (TCs) trigger endothelial barrier disruption and promote trans-endothelial migration of CFSE-labeled TCs. BBR treatment effectively ameliorates TC-induced endothelial disruption, an effect that is diminished by inhibiting transforming growth factor-β receptor 1 (TGFBR1). Blocking TGFBR1 blunts the anti-metastatic effect of BBR in vivo. Mechanistically, BBR binds to the intercellular portion of TGFBR1, suppresses its enzyme activities, and protects endothelial barrier disruption by TCs which express higher levels of TGF-β1. Hence, BBR might be a promising drug for reducing PC lung metastasis in clinical practice.
Multimodal Handheld Probe for Characterizing Otitis Media — Integrating Raman Spectroscopy and Optical Coherence Tomography
Authors: Monroy Guillermo L. , Fitzgerald Sean T. , Locke Andrea , Won Jungeun , Spillman, Jr. Darold R. , Ho Alexander , Zaki Farzana R. , Choi Honggu , Chaney Eric J. , Werkhaven Jay A. , Mason Kevin M. , Mahadevan-Jansen Anita , Boppart Stephen A.
Published: June 2022

Otitis media (OM) is a common disease of the middle ear, affecting 80% of children before the age of three. The otoscope, a simple illuminated magnifier, is the standard clinical diagnostic tool to observe the middle ear. However, it has limited contrast to detect signs of infection, such as clearly identifying and characterizing middle ear fluid or biofilms that accumulate within the middle ear. Likewise, invasive sampling of every subject is not clinically indicated nor practical. Thus, collecting accurate noninvasive diagnostic factors is vital for clinicians to deliver a precise diagnosis and effective treatment regimen. To address this need, a combined benchtop Raman spectroscopy (RS) and optical coherence tomography (OCT) system was developed. Together, RS-OCT can non-invasively interrogate the structural and biochemical signatures of the middle ear under normal and infected conditions.In this paper, in vivo RS scans from pediatric clinical human subjects presenting with OM were evaluated in parallel with RS-OCT data of physiologically relevant in vitro ear models. Component-level characterization of a healthy tympanic membrane and malleus bone, as well as OM-related middle ear fluid, identified the optimal position within the ear for RS-OCT data collection. To address the design challenges in developing a system specific to clinical use, a prototype non-contact multimodal handheld probe was built and successfully tested in vitro. Design criteria have been developed to successfully address imaging constraints imposed by physiological characteristics of the ear and optical safety limits. Here, we present the pathway for translation of RS-OCT for non-invasive detection of OM.
Construction of Chromosome Segment Substitution Lines and Inheritance of Seed-Pod Characteristics in Wild Soybean
Authors: Zheng Haiyang , Hou Lilong , Xie Jianguo , Cao Fubin , Wei Ruru , Yang Mingliang , Qi Zhaoming , Zhu Rongsheng , Zhang Zhanguo , Xin Dawei , Li Candong , Liu Chunyan , Jiang Hongwei , Chen Qingshan
Published: June 2022

Genetic populations provide the basis for genetic and genomic research, and chromosome segment substitution lines (CSSLs) are a powerful tool for the fine mapping of quantitative traits, new gene mining, and marker-assisted breeding. In this study, 213 CSSLs were obtained by self-crossing, backcrossing, and marker-assisted selection between cultivated soybean (Glycine max [L.] Merr.) variety Suinong14 (SN14) and wild soybean (Glycine soja Sieb. et Zucc.) ZYD00006. The genomes of these 213 CSSLs were resequenced and 580,524 single-nucleotide polymorphism markers were obtained, which were divided into 3,780 bin markers. The seed-pod-related traits were analyzed by quantitative trait locus (QTL) mapping using CSSLs. A total of 170 QTLs were detected, and 32 QTLs were detected stably for more than 2 years. Through epistasis analysis, 955 pairs of epistasis QTLs related to seed-pod traits were obtained. Furthermore, the hundred-seed weight QTL was finely mapped to the region of 64.4 Kb on chromosome 12, and Glyma.12G088900 was identified as a candidate gene. Taken together, a set of wild soybean CSSLs was constructed and upgraded by a resequencing technique. The seed-pod-related traits were studied by bin markers, and a candidate gene for the hundred-seed weight was finely mapped. Our results have revealed the CSSLs can be an effective tool for QTL mapping, epistatic effect analysis, and gene cloning.
Prediction of Liquid Magnetization Series Data in Agriculture Based on Enhanced CGAN
Authors: Nie Jing , Wang Nianyi , Li Jingbin , Wang Yi , Wang Kang
Published: June 2022

The magnetized water and fertilizer liquid can produce biological effect of magnetic field on crops, but its residual magnetic field strength is difficult to be expressed quantitatively in real time, and accurate prediction of it is helpful to define the scope of action of liquid magnetization. In this paper, a prediction model for liquid magnetization series data is presented. It consists of conditional generative adversarial network (CGAN) and projected gradient descent (PGD) algorithm. First, the real training dataset is used as the input of PGD attack algorithm to generate antagonistic samples. These samples are added to the training of CGAN as true samples for data enhancement. Second, the training dataset is used as both the generator and discriminator input of CGAN to constrain the model, capture distribution of the real data. Third, a network model with three layers of CNN is built and trained inside CGAN. The input model is constructed by using the structure of two-dimensional convolution model to predict data. Lastly, the performance of the model is evaluated by the error between the final generated predicted value and the real value, and the model is compared with other prediction models. The experimental results show that, with limited data samples, by combining PGD attack with CGAN, the distribution of the real data can be more accurately captured and the data can be generated to meet the actual needs.
Post-flowering Soil Waterlogging Curtails Grain Yield Formation by Restricting Assimilates Supplies to Developing Grains
Authors: Ma Shangyu , Hou Junyou , Wang Yanyan , Wang Mengchang , Zhang Wenjing , Fan Yonghui , Huang Zhenglai
Published: June 2022

Soil waterlogging is among the major factors limiting the grain yield of winter wheat crops in many parts of the world, including the middle and lower reaches of the Yangtze River China. In a field study, we investigated the relationship between leaf physiology and grain development under a varying duration of post-flowering waterlogging. A winter wheat cultivar Ningmai 13 was exposed to soil waterlogging for 0 (W0), 3 (W3), 6 (W6), and 9 d (W9) at anthesis. Increasing waterlogging duration significantly reduced flag leaf SPAD (soil plant analysis development) values and net photosynthetic rate (Pn). There was a linear reduction in flag leaf Pn and SPAD as plant growth progressed under all treatments; however, the speed of damage was greater in the waterlogged leaves. For example, compared with their respective control (W0), flag leaves of W9 treatment have experienced 46% more reduction in Pn at 21 d after anthesis (DAA) than at 7 DAA. Increasing waterlogging duration also induced oxidative damage in flag leaves, measured as malondialdehyde (MDA) contents. The capacity to overcome this oxidative damage was limited by the poor performance of antioxidant enzymes in wheat leaves. Inhibited leaf Pn and capacity to sustain assimilate synthesis under waterlogged environments reduced grain development. Compared with W0, W6 and W9 plants experienced a 20 and 22% reduction in thousand grain weight (TGW) in response to W6 and W9, respectively at 7 DAA and 11 and 19%, respectively at 28 DAA. Sustained waterlogging also significantly reduced grain number per spike and final grain yield. Averaged across two years of study, W9 plants produced 28% lesser final grain yield than W0 plants. Our study suggested that wheat crops are highly sensitive to soil waterlogging during reproductive and grain filling phases due to their poor capacity to recover from oxidative injury to photosynthesis. Management strategies such as planting time, fertilization and genotype selection should be considered for the areas experiencing frequent waterlogging problems.
Academic Management in Uncertain Times: Shifting and Expanding the Focus of Cognitive Load Theory During COVID-19 Pandemic Education
Authors: Gould Douglas J. , Sawarynski Kara , Mohiyeddini Changiz
Published: June 2022

Globally, the COVID-19 pandemic has forced medical education toward more “online education” approaches, causing specific implications to arise for medical educators and learners. Considering an unprecedented and highly threatening, constrained, and confusing social and educational environment caused by the COVID-19 pandemic, we decided to shift the traditional focus of the Cognitive Load Theory (CLT) from students to instructors. In this process, we considered recent suggestions to acknowledge the psychological environment in which learning happens. According to this fundamental fact, “Learning and instructional procedures do not occur in a situational vacuum.” Following this assertion, we adapted and implemented principles of CLT to reduce the extraneous load for our faculty to facilitate continued scholarly activity and support the overall wellbeing of our faculty during these trying times. The adoption of these principles enabled our team to cultivate attitudes and skills across multiple domains, such as online presentation technologies, implementing and maintaining a “classroom atmosphere” in a virtual environment, encouraging discussion among large online groups of students, facilitating group work, providing virtual office hours, and proactively planning for subsequent sessions.
“You Don’t Know Me so Don’t Try to Judge Me”: Gender and Identity Performance on Social Media Among Young Indian Users
Authors: Majumdar Sramana , Tewatia Maanya , Jamkhedkar Devika , Bhatia Khushi
Published: June 2022

Social media is the preferred communication platform for today’s youth, yet little is known of how online intergender communication is shaped by social identity norms. Drawing from the Social Identity and Deindividuation Effects (SIDE) approach, we argue that through depersonalization, online interactions are marked by the salience of social identities and identity performance conforming to perceived norms of behavior (traditional as well as developing). We specifically look at discursive terms and their meaning-making as a strategic performance of gender in uncontrolled social media interactions. We examined a corpus of 442 comments from selected public Indian Facebook pages in two phases over a span of 1 year (2020–2021). Thematic discourse analysis revealed established (#mansplaining, pseudofeminism) and emerging (choice feminism, MGTOW, #fuckboi etc.) discursive strategies within the major themes on feminism and antifeminism, men’s rights, intersectional feminism, and sexual behavior. These meaningful terms are used to modulate identity performance in a heavily contested space, reflecting both consolidation as well as mobilization functions, as proposed by SIDE. The findings highlight that intergender communication on social media is both dependent on existing offline norms, while challenging the same to create new discourses of gender.
Influence of Tactical and Situational Variables on Offensive Sequences During Elite European Handball Matches
Authors: Ferrari Willian , Sarmento Hugo , Marques Adilson , Dias Gonçalo , Sousa Tiago , Sánchez-Miguel Pedro Antonio , Gama José , Vaz Vasco
Published: June 2022

The main objective of this study was to analyze the influence of tactical and situational variables on offensive sequences during elite European handball matches. A sample of 55 games and 5.857 offensive sequences from the European Handball Federation Champions League, the selected teams were classified as the top eight teams in the league, were analyzed usingX2and logistic regression analyses. Results indicated that positional attacks [odds ratio (OR) = 0.34; 95% CI: 0.28–0.42;p < 0.001] and fast attacks (OR = 0.46; 95% CI: 0.36–0.57;p < 0.001) decreased the probability of success for an offensive sequence by 66% and 54% when compared with counterattacks. Offensive sequences that start in the attacking zone seem to be less effective (~78%) than those that start from a situation of “ball in center.” Additionally, offensive sequences that finished in the defensive zone of the observed team were 3.19 times more effective than those that ended before the 9 m zone. We concluded that compared with offensive sequences where the shot is performed from the 9 m zone, the chances of an offensive sequence ending successfully are 3.65, 3.60, and 2.21 times higher, for offensive sequences where the shot is performed from 9 m, 6 m, and the defensive zone, respectively. On the other hand, many variables seem to impact the performance of handball teams. Nevertheless, a significant challenge remains, and more research needs to be conducted to analyze the obstacles that teams need to overcome while attacking in the most effective way possible.
Influence of Social Media on Corporate Communication Social Responsibility Under Entrepreneurial Psychology
Authors: Cai Mufei
Published: June 2022

The research intends to improve the optimization of social media’s nature of corporate social responsibility (CSR) and standardize the influence mechanism of social media. First, the research analyzes the concepts of social media, CSR, and corporate reputation from the perspective of entrepreneurial psychology and expounds on the influencing factors of CSR scores from a macro perspective. Second, the mechanism of social media’s role in CSR is briefly discussed. On this basis, it is found that the most intuitive manifestation of social media platforms affecting the communication of CSR is the impact on the score of CSR. Three hypotheses are proposed, namely, (1) social media platforms, such as “WeChat,” have a greater impact on the communication of CSR; (2) social media is positively correlated with the increase in CSR scores; and (3) user reading and liking are positively correlated with the increase in CSR scores. Finally, 78 listed companies related to the mobile communication industry are selected as samples for the questionnaire survey and statistical analysis, and the hypothesis is demonstrated. The results demonstrate that the hypothesis that the social media platform “WeChat” has an impact on the communication of CSR is valid, and the hypothesis that the open state of “social media” is positively correlated with the increase in CSR scores is not valid. It is assumed that the number of user readings and likes is positively correlated with the increase in CSR score, which is valid under a limited sample. It is concluded that the WeChat platform has the best effect on the communication of CSR and can provide the impetus for the improvement of corporate reputation. The opening of “social media” is not directly related to the improvement of CSR scores. The correct operation of “social media” will have an impact on the communication of CSR. The correlation between the number of users reading and liking on “social media” and the increase in CSR score is not significant, as only the number of “likes” on social media related to shareholders is significantly associated with an increase in CSR score. The linear regression coefficient between the number of likes and the increase in CSR score is less than 0.05. There is a positive correlation between user reads, likes, and increases in CSR scores. This research helps enterprises to effectively fulfill their social responsibilities and improve the efficiency of CSR. This makes up for the lack of social media’s influence mechanism on the nature of CSR. It innovatively explores the impact of social media on CSR from the perspective of entrepreneurial psychology and provides some ideas for entrepreneurs and enterprises to create CSR and CSR value.
The Correlation Between Quality of Life and Positive Psychological Resources in Cancer Patients: A Meta-Analysis
Authors: Zhao Xinxin , Tong Siqi , Yang Ye
Published: June 2022

Purpose

This study aimed to assess the evidence of the association between positive psychological resources and quality of life in patients with cancer.

Methods

Electronic searching was performed to retrieve articles from PubMed, Web of Science, Embase, CNKI, and CBM (from inception to 7 April 2022). Summary correlation coefficient (r) values were extracted from each study, and 95% CIs were calculated by the random-effect model. Subgroup and sensitivity analyses were performed to investigate potential heterogeneity.

Results

In total, sixty-six articles were included in the present study. The pooled r for resilience was 0.71 (95%CI: 0.55, 0.87), hope 0.50 (95%CI: 0.43, 0.56), self-efficacy 0.53 (95%CI: 0.46, 0.61), self-esteem 0.46 (95%CI: 0.28, 0.63), and optimism 0.30 (95%CI: 0.19, 0.40). For subgroup analysis, no significant differences were found between minors and adults.

Conclusion

This study indicated that resilience, hope, optimism, self-esteem, and self-efficacy were positively correlated with quality of life in patients with cancers. Therefore, intervention programs should be focused on increasing state-like positive psychological resources to improve the quality of life in patients with cancer.
Future Objectivity Requires Perspective and Forward Combinatorial Meta-Analyses
Authors: Hanfstingl Barbara
Published: June 2022

This manuscript contributes to a future definition of objectivity by bringing together recent statements in epistemology and methodology. It outlines how improved objectivity can be achieved by systematically incorporating multiple perspectives, thereby improving the validity of science. The more result-biasing perspectives are known, the more a phenomenon of interest can be disentangled from these perspectives. Approaches that call for the integration of perspective into objectivity at the epistemological level or that systematically incorporate different perspectives at the statistical level already exist and are brought together in the manuscript. Recent developments in research methodology, such as transparency, reproducibility of research processes, pre-registration of studies, or free access to raw data, analysis strategies, and syntax, promote the explication of perspectives because they make the entire research process visible. How the explication of perspectives can be done practically is outlined in the manuscript. As a result, future research programs can be organized in such a way that meta-analyses and meta-meta-analyses can be conducted not only backward but forward and prospectively as a regular and thus well-prepared part of objectification and validation processes.
An Exploratory Study of Strategy Use on Elicited Imitation Tasks
Authors: Lei Yuyun , Yan Xun
Published: June 2022

Elicited imitation (EI) has gained popularity with recent interests in the quest for efficient and flexible measures of second language (L2) proficiency. Despite the surge of interests, questions remain as to what specific linguistic knowledge, skills, and strategies EI measures. To contribute to this line of inquiry, this study explored the nature of strategy use and its effect on EI performance to elucidate the constructs of EI. Twenty-four L2 learners and eight native speakers of Chinese completed an EI test of Chinese and a strategy use questionnaire after the test. Qualitative analyses of the questionnaire responses revealed that participants mainly employed five types of strategies, including approach strategies, cognitive strategies, metacognitive strategies, communication strategies, and test-wiseness strategies. While native speakers reported the least number of strategies, higher-proficiency L2 learners reported more strategies than lower-proficiency L2 learners. We further subjected strategy use, along with participant proficiency level, item length, and item complexity level, to linear mixed-effects regression analyses. The results showed that participant proficiency level, item length, and item complexity level explained the largest test score variance; in contrast, strategy use of different types only accounted for a smaller proportion. The total number of cognitive strategies had a significant, positive effect on EI performance whereas the total number of metacognitive strategies had a significant, negative effect. These findings offer some insights into the nature of speech comprehension and production on EI and provide validity evidence for the use of EI as a language proficiency measure.