bluuebunny/crossref_metadata_2025 huggingface dataset:
Identify the Prognostic and Immune Profile of VSIR in the Tumor Microenvironment: A Pan-Cancer Analysis
Authors: Liu Yuanyuan , Zhang Jingwei , Wang Zeyu , Zhang Xun , Dai Ziyu , Wu Wantao , Zhang Nan , Liu Zaoqu , Zhang Jian , Luo Peng , Wen Zhipeng , Yu Jing , Zhang Hao , Yang Tubao , Cheng Quan
Published: April 2022

VSIR is a critical immunomodulatory receptor that inhibits T cell effector function and maintains peripheral tolerance. However, the mechanism by which VSIR participates in tumor immunity in the pan-cancer tumor microenvironment remains unclear. This study systematically explored the prognostic and immune profile of VSIR in the tumor microenvironment of 33 cancers. We compared the expression patterns and molecular features of VSIR in the normal and cancer samples both from the public databases and tumor chips. VSIR level was significantly related to patients’ prognosis and could be a promising predictor in many tumor types, such as GBM, KIRC, SKCM, READ, and PRAD. Elevated VSIR was closely correlated with infiltrated inflammatory cells, neoantigens expression, MSI, TMB, and classical immune checkpoints in the tumor microenvironment. Enrichment signaling pathways analysis indicated VSIR was involved in several immune-related pathways such as activation, proliferation, and migration of fibroblast, T cell, mast cell, macrophages, and foam cell. In addition, VSIR was found to widely express on cancer cells, fibroblasts, macrophages, and T cells in many tumor types based on the single-cell sequencing analysis and co-express with M2 macrophage markers CD68, CD163 based on the immunofluorescence staining. Finally, we predicted the sensitive drugs targeting VSIR and the immunotherapeutic value of VSIR. In sum, VSIR levels strongly correlated with the clinical outcome and tumor immunity in multiple cancer types. Therefore, therapeutic strategies targeting VSIR in the tumor microenvironment may be valuable tools for cancer immunotherapy.
The Role of Syncytin in Placental Angiogenesis and Fetal Growth
Authors: Wang Ya-Nan , Ye Yixin , Zhou Da , Guo Ze-Wen , Xiong Zhelei , Gong Xing-Xing , Jiang Shi-Wen , Chen Haibin
Published: April 2022

Background: Syncytin, a retroviral envelope protein, is specifically expressed on trophoblast cells and mediates formation of the syncytiotrophoblast through fusogenic activity. Decreased expression of Syncytin was found in fetal growth restriction placentas.

Results: By generating an inducible knockout of the syncytin-a gene in mice, we show a specific disruption of placental angiogenesis with abnormal formation of two syncytiotrophoblast layers. Consistent with the defects observed in vivo, conditioned medium collected from trophoblast cells, following Syncytin-1 knockdown, contains lower expression of vascular endothelial growth factor and placental growth factor, and higher levels of soluble fms-like protein kinase-1 in BeWo and HTR-8/SVneo cells which related with suppressed PI3K/Akt/mTOR pathway, and is reduced in ability to induce tube formation by HUVECs.

Conclusion: Syncytin participates in angiogenesis during placental development was first identified both in vivo and in vitro. Here, we give a new sight on understanding syncytin and pathophysiology of placenta related disease such as fetal growth restriction.
Stratification From Heterogeneity of the Cell-Death Signal Enables Prognosis Prediction and Immune Microenvironment Characterization in Esophageal Squamous Cell Carcinoma
Authors: Zhang Yiyuan , Chen Yanxing
Published: April 2022

Esophageal squamous cell carcinoma (ESCC) is the primary subtype of esophageal cancer (EC) characterized by a high incidence rate and extremely poor prognosis worldwide. Previous studies suggested that the specific cell death signal was linked to different immune subtypes in multiple cancers, while a comprehensive investigation on ESCC is to be performed yet. In the current study, we dissected different cell death signals in ESCC tumors and then integrated that functional information to stratify ESCC patients into different immunogenic cell death (ICD) subtypes. By systematically analyzing the transcriptomes of 857 patients and proteomic profile of 124 patients, we found that the signals of necroptosis, pyroptosis, and ferroptosis are positively associated with activated immunity in ESCC. We identified two ICD pattern terms, namely, ICD-high and ICD-low subtypes that positively correlated to both progression-free survival and overall survival. In addition, cell fraction deconvolution analysis revealed that more infiltrated leukocytes were enriched in ICD-high types, especially antigen-presenting cells, such as dendritic cells and macrophages. With the XGBoost algorithm, we further developed a 14-gene signature which can simplify the subtyping for allocating new samples, by which we validated the prognosis value of the signature and proved that the ICD score scheme could serve as a promising biomarker for stratifying patients with immunotherapy in several immune checkpoint blockade treatment cohorts. Collectively, we successfully constructed the ICD scheme, which enables predicting of the prognosis or immunotherapy efficacy in ESCC patients and uncovered the critical interplay between cell death signals and immune status in ESCC.
Nde1 and Ndel1: Outstanding Mysteries in Dynein-Mediated Transport
Authors: Garrott Sharon R. , Gillies John P. , DeSantis Morgan E.
Published: April 2022

Cytoplasmic dynein-1 (dynein) is the primary microtubule minus-end directed molecular motor in most eukaryotes. As such, dynein has a broad array of functions that range from driving retrograde-directed cargo trafficking to forming and focusing the mitotic spindle. Dynein does not function in isolation. Instead, a network of regulatory proteins mediate dynein’s interaction with cargo and modulate dynein’s ability to engage with and move on the microtubule track. A flurry of research over the past decade has revealed the function and mechanism of many of dynein’s regulators, including Lis1, dynactin, and a family of proteins called activating adaptors. However, the mechanistic details of two of dynein’s important binding partners, the paralogs Nde1 and Ndel1, have remained elusive. While genetic studies have firmly established Nde1/Ndel1 as players in the dynein transport pathway, the nature of how they regulate dynein activity is unknown. In this review, we will compare Ndel1 and Nde1 with a focus on discerning if the proteins are functionally redundant, outline the data that places Nde1/Ndel1 in the dynein transport pathway, and explore the literature supporting and opposing the predominant hypothesis about Nde1/Ndel1’s molecular effect on dynein activity.
Tubulin Post-translational Modifications: Potential Therapeutic Approaches to Heart Failure
Authors: Liu Chang , Chen Yuwen , Xie Yao , Xiang Meixiang
Published: April 2022

In recent decades, advancing insights into the mechanisms of cardiac dysfunction have focused on the involvement of microtubule network. A variety of tubulin post-translational modifications have been discovered to fine-tune the microtubules’ properties and functions. Given the limits of therapies based on conserved structures of the skeleton, targeting tubulin modifications appears to be a potentially promising therapeutic strategy. Here we review the current understanding of tubulin post-translational modifications in regulating microtubule functions in the cardiac system. We also discussed how altered modifications may lead to a range of cardiac dysfunctions, many of which are linked to heart failure.
Chlamydia pneumoniae Infection Induces Vascular Smooth Muscle Cell Migration and Atherosclerosis Through Mitochondrial Reactive Oxygen Species-Mediated JunB-Fra-1 Activation
Authors: Zhao Xi , Miao Guolin , Zhang Lijun , Zhang Yuke , Zhao Huanhuan , Xu Zhelong , Wang Beibei , Zhang Lijun
Published: April 2022

Infection is closely related to atherosclerosis, which is a major pathological basis for cardiovascular diseases. Vascular smooth muscle cell (VSMC) migration is an important trigger in development of atherosclerosis that is associated with Chlamydia pneumoniae (C. pneumoniae) infection. However, the mechanism of VSMC migration remains unclear, and whether antioxidant could be a therapeutic target for C. pneumoniae infection-induced atherosclerosis also remains unknown. The results showed that C. pneumoniae infection mainly impaired mitochondrial function and increased the level of mitochondrial reactive oxygen species (mtROS). The expressions of protein JunB, Fra-1 and Matrix metalloproteinase 2 (MMP) evidently increased after C. pneumoniae infection, and the interaction between JunB and Fra-1 was also enhanced. After scavenging mtROS by antioxidant Mito-TEMPO, the increasing expressions of JunB, Fra-1, MMP2 and the capacity of VSMC migration induced by C. pneumoniae infection were all inhibited. In comparison with infected ApoE-/- mice, the level of ROS in atherosclerotic lesion in ApoE-/-TLR2-/- mice with C. pneumoniae infection decreased. Knocking out TLR2 suppressed the expressions of JunB, Fra-1 and MMP2 in VSMCs and the formation of atherosclerotic lesion after C. pneumoniae infection. Furthermore, after using small interfering RNA to inhibit the expression of TLR2, the level of mtROS and the expressions of JunB, Fra-1 and MMP2 apparently decreased. Taken together, C. pneumoniae infection may promote VSMC migration and atherosclerosis development by increasing the level of mtROS through TLR2 to activate the JunB-Fra-1/MMP2 signaling pathway. The data provide the first evidence that antioxidant could reduce C. pneumoniae infection-induced VSMC migration and atherosclerosis.
Flash Synthesis and Continuous Production of C-Arylglycosides in a Flow Electrochemical Reactor
Authors: Takumi Masahiro , Nagaki Aiichiro
Published: April 2022

Electrochemistry provides a green and atom-efficient route to synthesize pharmaceutical and useful functional molecules, as it eliminates the need for the harsh chemical oxidants and reductants commonly used in traditional chemical reactions. To promote the implementation of electrochemical processes in the industry, there is a strong demand for the development of technologies that would allow for scale-up and a shortened reaction process time. Herein, we report that electrolysis was successfully accomplished using a flow-divided-electrochemical reactor within a few seconds, enabling the desired chemical conversion in a short period of time. Moreover, the narrow electrode gap of the flow reactor, which offers greener conditions than the conventional batch reactor, resulted in the continuous flash synthesis of C-arylglycosides.
Targeting Multiresistant Gram-Positive Bacteria by Ruthenium, Osmium, Iridium and Rhodium Half-Sandwich Type Complexes With Bidentate Monosaccharide Ligands
Authors: Balázs Bence , Tóth Zoltán , Kacsir István , Sipos Adrienn , Buglyó Péter , Somsák László , Bokor Éva , Kardos Gábor , Bai Péter
Published: April 2022

Bacterial resistance to antibiotics is an ever-growing problem in heathcare. We have previously identified a set of osmium(II), ruthenium(II), iridium(III) and rhodium(III) half-sandwich type complexes with bidentate monosaccharide ligands possessing cytostatic properties against carcinoma, lymphoma and sarcoma cells with low micromolar or submicromolar IC50 values. Importantly, these complexes were not active on primary, non-transformed cells. These complexes have now been assessed as to their antimicrobial properties and found to be potent inhibitors of the growth of reference strains of Staphylococcus aureus and Enterococcus faecalis (Gram-positive species), though the compounds proved inactive on reference strains of Pseudomonas aerugonisa, Escherichia coli, Candida albicans, Candida auris and Acinetobacter baumannii (Gram-negative species and fungi). Furthermore, clinical isolates of Staphylococcus aureus and Enterococcus sp. (both multiresistant and susceptible strains) were also susceptible to the organometallic complexes in this study with similar MIC values as the reference strains. Taken together, we identified a set of osmium(II), ruthenium(II), iridium(III) and rhodium(III) half-sandwich type antineoplastic organometallic complexes which also have antimicrobial activity among Gram-positive bacteria. These compounds represent a novel class of antimicrobial agents that are not detoxified by multiresistant bacteria suggesting a potential to be used to combat multiresistant infections.
Electron Transfer Strategies to Regulate Carriers’ Separation for Intensive Pyroelectric Dynamic Therapy With Simultaneous Photothermal Therapy
Authors: Sun Bingxia , Meng Yun , Song Tianlin , Shi Jieyun , He Xinhong , Zhao Peiran
Published: April 2022

Endogenic heat shock proteins and uneven local heat distribution are two main problems in traditional tumor hyperthermia therapy strategies. Aiming at solving these problems, we designed Au–SnSe–PVP nanomaterials (ASNPs) by modifying Au nanoparticles (Au-NPs) and biocompatible PVP on SnSe nanorods via a new reactive oxygen species production strategy. The ASNPs with excellent photothermal conversion performance can produce thermoelectric effects in response to temperature differences during photothermal conversion. The modification of Au-NPs can attract free electron (e–) to accumulate and promote the separation of e– and holes (h+) in the thermoelectric process, thereby further promoting e–-rich Au-NPs-induced H2O2 homolysis and h+–H2O half-reaction to generate hydroxyl radicals, realizing the synergistic application of photothermal therapy and pyroelectric dynamic therapy in tumor treatment.
French Sign Language/French Translation Studies in France: A Mini Review
Authors: Burgat Sandrine , Encrevé Florence , Segouat Jérémie
Published: April 2022

In this mini review, we present an overview of existing research in French sign language (LSF)/French translation studies in France. The practice of LSF/French interpreting is ancient, since traces of it can be found in the High Middle Ages, but its professionalization is more recent (it dates from the Deaf Revival of 1975–1995). The profession of French to LSF translator is even more recent: it dates from about 10 years ago. Following this professionalization, translation studies research emerged about 30 years ago. It has been developing particularly over the last 20 years, driven by university programs (MA in LSF/French interpretation and translation). This research is multifaceted and is not confined to a single discipline. Indeed, translation studies are inherently multidisciplinary, and we can find references to translation and interpretation in historic, linguistic, sociologic, or computer science studies, among others. Moreover, translation studies are also part of different schools of thought, which can be explained historically. In this paper, we present an overview of translation research concerning LSF/French interpretation and translation (practiced by both deaf and hearing people) in France. We also address the prospects for further developments, related to the emergence of new practices, and the question of the didactic applications of these different researches in the field of interpreters and translators training within 5 universities in France.
Relationship Between Resting Heart Rate and Microalbuminuria in Adults With Hypertension: National Health and Nutrition Examination Survey 2009–2018
Authors: Peng Xiaodong , Li Yukun , Wang Xuesi , Ruan Yanfei , Liu Nian
Published: April 2022

Background

The impact of elevated resting heart rate on early-stage renal dysfunction, manifesting as microalbuminuria, in hypertension is unclear. This study aimed to analyze the association between resting heart rate and microalbuminuria in patients with hypertension according to their blood pressure status. In addition, the effect of antihypertensive agents on this relationship was evaluated.

Methods and Results

We searched the National Health and Nutrition Examination Survey for eligible participants from 2009 to 2018. Data on key parameters such as age, sex, blood pressure, heart rate, albumin creatinine ratio, and medication were collected for analysis. Subsequently, participants were classified according to the heart rate quartile and blood pressure status for subgroups assessment. A total of 5,692 participants were enrolled in this study. After adjusting the confounding factors, there was a linear association between resting heart rate and microalbuminuria in patients with hypertension (OR 1.184 [per 1 SD]; 95% CI: 1.101, 1.274; P < 0.001). However, the association between elevated resting heart rate and microalbuminuria was not significant in patients with uncontrolled hypertension (OR 1.092 [per 1 SD]; 95% CI: 0.935, 1.275; P = 0.269). The OR of the indirect effect of β-blockers on the risk of microalbuminuria incidence through heart rate was 0.926 (95% CI: 0.895, 0.956), while the direct effect was 1.374 (95% CI: 1.138, 1.662, P = 0.010). Similarly, dihydropyridine calcium channel blockers were associated with a higher prevalence of microalbuminuria (OR 1.300, 95% CI: 1.058, 1.597, P = 0.013), but the association between non-dihydropyridine calcium channel blockers and microalbuminuria was not significant (OR 1.207, 95% CI: 0.737, 1.978, P = 0.454).

Conclusion

Elevated resting heart rate is associated with a high risk of microalbuminuria in untreated patients and patients with controlled hypertension. Although there is a linear association between heart rate and microalbuminuria, the use of β-blockers exhibits a significantly increase in the prevalence of microalbuminuria in hypertension. Likewise, dihydropyridine calcium channel blockers may increase the risk of microalbuminuria in hypertension.
Outcomes With Multidisciplinary Cardiac Rehabilitation in Post-acute Systolic Heart Failure Patients—A Retrospective Propensity Score-Matched Study
Authors: Chen Shyh-Ming , Wang Lin-Yi , Liaw Mei-Yun , Wu Ming-Kung , Wu Po-Jui , Wei Chin-Ling , Chen An-Ni , Su Tsui-Ling , Chang Jui-Kun , Yang Tsung-Hsun , Chen Ching , Cheng Cheng-I , Chen Po-Cheng , Chen Yung-Lung
Published: April 2022

Background

Cardiac rehabilitation (CR) is recommended for patients with acute heart failure (HF). However, the results of outcome studies and meta-analyses on CR in post-acute care are varied. We aimed to assess the medium- to long-term impact of CR and ascertain the predictors of successful CR.

Methods

In this propensity score-matched retrospective cohort study, records of consecutive patients who survived acute HF (left ventricular ejection fraction <40) and participated in a multidisciplinary HF rehabilitation program post-discharge between May 2014 and July 2019 were reviewed. Patients in the CR group had at least one exercise session within 3 months of discharge; the others were in the non-CR group. After propensity score matching, the primary (all-cause mortality) and secondary (HF readmission and life quality assessment) outcomes were analyzed.

Results

Among 792 patients, 142 attended at least one session of phase II CR. After propensity score matching for covariates related to HF prognosis, 518 patients were included in the study (CR group, 137 patients). The all-cause mortality rate was 24.9% and the HF rehospitalization rate was 34.6% in the median 3.04-year follow-up. Cox proportional hazard analysis revealed that the CR group had a significant reduction in all-cause mortality compared to the non-CR group (hazard ratio [HR]: 0.490, 95% confidence interval [CI]: 0.308–0.778). A lower risk of the primary outcome with CR was observed in patients on renin-angiotensin-aldosterone system (RAAS) inhibitors, but was not seen in patients who were not prescribed this class of medications (interaction p = 0.014).

Conclusions

Cardiac rehabilitation participation was associated with reduced all-cause mortality after acute systolic heart failure hospital discharge. Our finding that the benefit of CR was decreased in patients not prescribed RAAS inhibitors warrants further evaluation.
Non-fasting Changes in Blood Lipids After Three Daily Meals Within a Day in Chinese Inpatients With Cardiovascular Diseases
Authors: Tan Yangrong , Lin Qiuzhen , Xu Jin , Zhu Liyuan , Guo Liling , Xie Yingying , Du Xiao , Zhang Shilan , Wen Tie , Liu Ling
Published: April 2022

Background

Non-fasting (i.e., postprandial) lipid detection is recommended in clinical practice. However, the change in blood lipids in Chinese patients with cardiovascular diseases after three daily meals has never been reported yet.

Methods

Serum levels of blood lipids were measured or calculated in 77 inpatients (48 men and 29 women) at high or very high risk of atherosclerotic cardiovascular disease (ASCVD) in the fasting state and at 4 h after three meals within a day according to their diet habits.

Results

Female patients showed significantly higher level of high-density lipoprotein cholesterol (HDL-C) than male patients, and the gender difference in other lipid parameters did not reach statistical significance at any time-point. Levels of triglyceride (TG) and remnant cholesterol (RC) increased, while that of low-density lipoprotein cholesterol (LDL-C) decreased significantly after three meals (p < 0.05). Levels of HDL-C, total cholesterol (TC), and non-high-density lipoprotein cholesterol (non-HDL-C) showed smaller changes after three meals. Percent reductions in the non-fasting LDL-C levels after lunch and supper were around 20%, which were greater than that after breakfast. The percent reductions in the non-fasting non-HDL-C levels after three meals were smaller than those in the non-fasting LDL-C levels. Patients with TG level ≥ 2.0 mmol/L (177 mg/dL) after lunch had significantly greater absolute reduction of LDL-C level than those with TG level < 2.0 mmol/L (177 mg/dL) after lunch [–0.69 mmol/L (–27 mg/dL) vs. –0.36 mmol/L (–14 mg/dL), p<0.01]. There was a significant and negative correlation between absolute change in LDL-C level and that in TG level (r = −0.32) or RC level (r = −0.67) after lunch (both p<0.01).

Conclusion

LDL-C level decreased significantly after three daily meals in Chinese patients at high or very high risk of ASCVD, especially when TG level reached its peak after lunch. Relatively, non-HDL-C level was more stable than LDL-C level postprandially. Therefore, when LDL-C level was measured in the non-fasting state, non-HDL-C level could be evaluated simultaneously to reduce the interference of related factors, such as postprandial hypertriglyceridemia, on detection.
Transfemoral Occlusion of Doubly Committed Subarterial Ventricular Septal Defect Using the Amplatzer Duct Occluder-II in Children
Authors: Tang Changqing , Zhou Kaiyu , Shao Shuran , Liu Xiaoliang , Li Yifei , Hua Yimin , Wang Chuan
Published: April 2022

Backgrounds

The traditional treatment of doubly committed subarterial ventricular septal defect (dcVSD) is open-heart surgery. This study aimed to evaluate the feasibility, safety, and outcome of transcatheter closure of small dcVSD using Amplatzer duct occluder-II (ADO-II) in children.

Methods

Between January 2016 and April 2021, 24 children (17 male and 7 female patients) with small dcVSD who received transfemoral closure with ADO-II were enrolled retrospectively. All of their available clinical and follow-up data were evaluated.

Results

The patients' median age was 3.2 years (1.6–12.6 years, 4.2 ± 3.1 years) and body weight was 13.3 kg (10.0–38.5 kg, 16.5 ± 7.7 kg). Left ventricular angiography showed that the median dcVSD size was 2.0 mm (1.5–3.5 mm, 2.1 ± 0.6 mm). The device was successfully implanted in 23 patients (95.8%), and one patient failed to be closed because of the underestimation of defect size due to preoperative aortic valve prolapse, with 16 patients by the antegrade approach and eight patients by retrograde approach. The diameters of the device used were 3/4, 4/4, and 5/4 mm. The median operative time was 40.0 min (20.0–75.0 min, 41.7 ± 13.7 min), and the median fluoroscopic time was 5.0 min (3.0–25.0 min, 6.8 ± 5.0 min). With a follow-up duration of 1+ to 45+ months, only 1 patient presented with new-onset mild aortic regurgitation (AR).

Conclusion

Transfemoral closure of small dcVSD with ADO-II is technically feasible and safe in the selected children. However, the development or worsening of AR requires long-term follow-up.
Dermal Lymphatic Capillaries Do Not Obey Murray's Law
Authors: Talkington Anne M. , Davis Reema B. , Datto Nicholas C. , Goodwin Emma R. , Miller Laura A. , Caron Kathleen M.
Published: April 2022

Lymphatic vessels serve as a major conduit for the transport of interstitial fluid, immune cells, lipids and drugs. Therefore, increased knowledge about their development and function is relevant to clinical issues ranging from chronic inflammation and edema, to cancer metastasis to targeted drug delivery. Murray's Law is a widely-applied branching rule upheld in diverse circulatory systems including leaf venation, sponge canals, and various human organs for optimal fluid transport. Considering the unique and diverse functions of lymphatic fluid transport, we specifically address the branching of developing lymphatic capillaries, and the flow of lymph through these vessels. Using an empirically-generated dataset from wild type and genetic lymphatic insufficiency mouse models we confirmed that branching blood capillaries consistently follow Murray's Law. However surprisingly, we found that the optimization law for lymphatic vessels follows a different pattern, namely a Murray's Law exponent of ~1.45. In this case, the daughter vessels are smaller relative to the parent than would be predicted by the hypothesized radius-cubed law for impermeable vessels. By implementing a computational fluid dynamics model, we further examined the extent to which the assumptions of Murray's Law were violated. We found that the flow profiles were predominantly parabolic and reasonably followed the assumptions of Murray's Law. These data suggest an alternate hypothesis for optimization of the branching structure of the lymphatic system, which may have bearing on the unique physiological functions of lymphatics compared to the blood vascular system. Thus, it may be the case that the lymphatic branching structure is optimized to enhance lymph mixing, particle exchange, or immune cell transport, which are particularly germane to the use of lymphatics as drug delivery routes.
Mapping and Ablation of Isolated Frequent Symptomatic Premature Atrial Contractions in Patients With Structurally Normal Heart
Authors: He Bo , Li Yi , Huang Weiping , Yu Wenxi , Zhao Fang , Wu Xiaoyan , Yao Shuyuan , Po Sunny S. , Lu Zhibing
Published: April 2022

Background

The present study investigated the safety and efficacy of mapping and ablating isolated premature atrial contractions (PACs) in patients with a structurally normal heart, as well as whether the elimination of PACs by radiofrequency catheter ablation (RFCA) improved symptoms and the quality of life.

Methods

Forty-three consecutive patients with frequent, symptomatic, and drug-refractory PACs, but without atrial tachyarrhythmias (≥5 beats), were enrolled. In all patients, we performed physical, laboratory, and imaging examinations to exclude structural heart disease. The quality of life questionnaire SF-36 before and 3 months after RFCA was performed in each patient.

Results

Twenty-three men and 20 women with an average age of 52.6 ± 17.6 years were finally enrolled. The mean number of PACs was 21,685 ± 9,596 per 24 h, and the mean PACs' burden was 28.9 ± 13.7%. Short runs of tachycardia (<5 atrial beats) were observed in 32 patients (74.4%). All patients underwent successful RFCA without complications. The activation time at the successful ablation sites preceded the onset of the P-wave by 36 ± 7.6 ms. During 15 ± 8 months of follow-up, the recurrence of PACs was observed in 2 patients. The 24-h PAC burden was significantly reduced 3 months after RFCA (mean 0.5%, p < 0.05). The quality of life scores were significantly increased 3 months after RFCA (all p < 0.05).

Conclusions

RFCA was feasible, safe, and effective to eliminate isolated frequent, symptomatic, and drug-refractory PACs in patients with a structurally normal heart. The elimination of PACs by RFCA significantly improved symptoms and the quality of life.
Applications and Techniques for Fast Machine Learning in Science
Authors: Deiana Allison McCarn , Tran Nhan , Agar Joshua , Blott Michaela , Di Guglielmo Giuseppe , Duarte Javier , Harris Philip , Hauck Scott , Liu Mia , Neubauer Mark S. , Ngadiuba Jennifer , Ogrenci-Memik Seda , Pierini Maurizio , Aarrestad Thea , Bähr Steffen , Becker Jürgen , Berthold Anne-Sophie , Bonventre Richard J. , Müller Bravo Tomás E. , Diefenthaler Markus , Dong Zhen , Fritzsche Nick , Gholami Amir , Govorkova Ekaterina , Guo Dongning , Hazelwood Kyle J. , Herwig Christian , Khan Babar , Kim Sehoon , Klijnsma Thomas , Liu Yaling , Lo Kin Ho , Nguyen Tri , Pezzullo Gianantonio , Rasoulinezhad Seyedramin , Rivera Ryan A. , Scholberg Kate , Selig Justin , Sen Sougata , Strukov Dmitri , Tang William , Thais Savannah , Unger Kai Lukas , Vilalta Ricardo , von Krosigk Belina , Wang Shen , Warburton Thomas K.
Published: April 2022

In this community review report, we discuss applications and techniques for fast machine learning (ML) in science—the concept of integrating powerful ML methods into the real-time experimental data processing loop to accelerate scientific discovery. The material for the report builds on two workshops held by the Fast ML for Science community and covers three main areas: applications for fast ML across a number of scientific domains; techniques for training and implementing performant and resource-efficient ML algorithms; and computing architectures, platforms, and technologies for deploying these algorithms. We also present overlapping challenges across the multiple scientific domains where common solutions can be found. This community report is intended to give plenty of examples and inspiration for scientific discovery through integrated and accelerated ML solutions. This is followed by a high-level overview and organization of technical advances, including an abundance of pointers to source material, which can enable these breakthroughs.
Ice Dynamics and Morphological Changes During Proglacial Lake Development at Exploradores Glacier, Patagonia
Authors: Irarrazaval Inigo , Dussaillant Alejandro , Vivero Sebastián , Iribarren-Anacona Pablo , Mariethoz Gregoire
Published: April 2022

Proglacial lakes are ubiquitous features formed during deglaciarization and are currently increasing in number in Patagonia and elsewhere. Proglacial lakes can affect glacier dynamics, catchment hydrology and have the potential to cause glacial lake outburst floods. Therefore, monitoring the onset and development of proglacial lake formation is relevant to understand glacial processes and anticipate glacier response to climate change. In this study, we integrate geomorphological and ice-dynamic information to assess proglacial lake development in Exploradores Glacier, Chilean Patagonia. We monitor recent spatial and temporal changes in the lower trunk of Exploradores Glacier (10 km2) to provide a 20-year observation record by combining eight uncrewed aerial vehicles (UAV) surveys between 2019 and 2020, with high-medium resolution satellite imagery (Rapid Eye and Landsat) between 2000 and 2018. We use feature tracking techniques, digital surface elevation model analysis and field data to create a multi-temporal scale (inter-annual and seasonal) and a multi-spatial (cm to km) data set. Our analysis shows that surface velocity overall trend has not changed over the last 20 years and that surface velocity near the terminus is significant (>10 m a−1). Moreover, an exceptional advance over moraine deposits was detected. We also found low downwasting rates (<0.5 m a−1) close to the glacier terminus which are attributed to sufficient ice flux and the insulation effect of the debris-covered surface. However, hundreds of supraglacial ponds were observed and are currently coalescing and expanding by ice-cliff backwasting favoring glacier disintegration. Lastly, it was found that calving losses at the east marginal lake equaled ice-flux input into the lake for the UAV monitored period. This study contributes to a better understanding of glacial lake dynamics during proglacial lake development, and our results may help ice modelling efforts to predict glacier response to future climate scenarios.
Climate Variations vs. Human Activities: Distinguishing the Relative Roles on Vegetation Dynamics in the Three Karst Provinces of Southwest China
Authors: Yang Hao , Hu Jing , Zhang Shuo , Xiong Ling , Xu Yong
Published: April 2022

Vegetation in karst areas is crucial for maintaining fragile local ecosystems, driven by climate change and human activities. Southwest China contains the largest continuous karst zone in the world and its vegetation dynamics are more sensitive to climate changes and human activities. However, previous studies rarely studied the driving roles of vegetation dynamics in karst areas during the last 20 years, and whether climate change or human disturbance factors have dominated the vegetation dynamics are still uncertain. The objective of this work is to study vegetation dynamics and its responses to climate change and human activities from 2001 to 2019 using the normalized difference vegetation index (NDVI). Taking the three karst provinces of southwest China as study area. Vegetation variation characteristics under the influence of climate variations and human activities were distinguished through a residual analysis. The results indicated general greening trends with about 90.31% of the study area experiencing an increase in NDVI and about 9.69% of the area showing a decrease. Within the combination of climate change and human activities, human activities became the dominant factor in the process of vegetation cover improvement and degradation in the study area during 2001–2019, with average relative roles of 62 and 59%, respectively. Temperature made the greatest positive contribution among the climatic factors, followed by precipitation and relative humidity. In contrast, sunshine duration had a negative effect on NDVI in the study area. Human activities have had different effects on the vegetation dynamics of the three karst provinces in southwest China, including destruction of vegetation around some cities.
Mechanism of Overlying Strata Migration and Failure During Underground Mining in the Mountainous Carbonate Areas in Southwestern China
Authors: Li Jun , Li Bin , Gao Yang , Cui Fangpeng , He Kai , Li Jiangshan , Li Haowen
Published: April 2022

In this study, theoretical analysis and numerical simulation methods were used to investigate the mechanical mechanism of the migration failure of the overlying strata and the development characteristics of the water-conducting fractured zone (WCFZ) in the mountainous carbonate areas in southwestern China. Due to the block-shaped rock mass structure characteristics of carbonate rocks, this paper considered the rock mass structure of the overlying strata. For the three-hinged arch structure of the block-shaped rock mass, the theory of damage mechanics was used to deduct the recursive calculation formula for the ultimate subsidence of the three-hinged arch structure of the overlying strata. Then, a method for determining the height of the WCFZ (HWCFZ) in the overlying strata under mining conditions was developed. Numerical simulations were carried out to study the stress field, plastic zone, and displacement field of the overlying strata and the dynamic evolution of the WCFZ during the mining process, and it was revealed that there was a positive feedback effect between them. After the mine was mined, due to the change of the stress field of the overlying strata, the overlying strata were mainly subjected to three types of plastic deformation during the fracturing process: tension, shearing, and tensile-shearing. There was a plastic partitioning phenomenon in the overlying strata. Among them, the tensile-slip failure zone was the most severely damaged. And the boundary of the WCFZ and the bending subsidence zone were determined based on the change characteristics of the displacement field. The HWCFZ obtained from the numerical simulations was consistent with the theoretical calculated value (93 vs. 92.5 m), validating the reliability and accuracy of the theoretical calculation method. Underground mining activities are active in the mountainous carbonate areas in southwestern China, and there are many landslide disasters due to overlying strata collapse, resulting in serious casualties. Therefore, prediction of HWCFZ and stability analysis of mountain need to be carried out for different mines in order to effectively carry out geological disaster prevention and mitigation research.
Study on Aerodynamic Performance of a Straight-Bladed VAWT Using a Wind-Gathering Device With Polyline Hexagonal Pyramid Shape
Authors: Li Yan , Tong Guoqiang , Zhao Bin , Feng Fang , Tagawa Kotaro
Published: April 2022

The self-starting performance and aerodynamic characteristics at low wind speeds are the two main problems for the straight-bladed vertical Axis wind turbine (SB-VAWT). In this study, a new kind of wind-gathering device (WGD), which can be installed up and down the rotor, with a polyline hexagonal pyramid shape, was proposed. Both the numerical simulation and the wind tunnel test were performed on this WGD. Based on the method of quadratic rotary orthogonal combination design, the effect of the main structural parameters of the WGD on the self-starting performance of the SB-VAWT was researched by 3D numerical simulation. The optimal combination of the main structural parameters for the WGD under the condition of this study was obtained. Furthermore, wind tunnel tests were carried out on the SB-VAWT with and without the WGD having the optimal structural parameters, including the starting performance, power performance, and rotational revolution performance. The results showed that both the static performance and dynamic performance of the SB-VAWT were increased to some degree by installing this kind of WGD. The improvement is more obvious under the condition of low wind speed. This research can be a useful reference for the in-depth study on the optimal WGDs for the SB-VAWTs.
Multivariate Time Series Prediction for Loss of Coolant Accidents With a Zigmoid-Based LSTM
Authors: Gong Shanshan , Yang Suyuan , She Jingke , Li Weiqi , Lu Shaofei
Published: April 2022

Post-LOCA prediction is of safety significance to NPP, but requires a processing coverage of non-linearity, both short and long-term memory, and multiple system parameters. To enable an ability promotion of previous LOCA prediction models, a new gate function called zigmoid is introduced and embedded to the traditional long short-term memory (LSTM) model. The newly constructed zigmoid-based LSTM (zLSTM) amplifies the gradient at the far end of the time series, which enhances the long-term memory without weakening the short-term one. Multiple system parameters are integrated into a 12-dimension input vector to the zLSTM for a comprehensive consideration based on which the LOCA prediction can be accurately generated. Experimental results show both accuracy evaluations and LOCA progression produced by the proposed zLSTM, and two baseline methods demonstrating the superiority of applying zLSTM to LCOA predictions.
Assessing Sources and Distribution of Heavy Metals in Environmental Media of the Tibetan Plateau: A Critical Review
Authors: Wang Wenjuan , Ji Xiaowen , Abakumov Evgeny , Polyakov Vyacheslav , Li Gensheng , Wang Dong
Published: April 2022

With a unique multi-sphere environmental system, the Tibetan Plateau (TP) plays an essential role in the ecological sheltering function for China and other parts of Asia. However, black carbon, persistent organic pollutants, and heavy metals (HMs) have been increased dramatically since the 1950s, reflecting rising emissions in Asia. In this context, the sources and distribution of HMs were summarized in the environment media of the TP. The results showed that 1) HMs in the TP may be generated from geogenic/pedogenic associations (Cu, Cr, Ni, As, and Co) and anthropogenic activities of local or long-distance atmospheric transmission (Cd, Pb, Zn, and Hg). 2) The atmospheric transport emission sources of HMs are mainly from the surrounding heavily-polluted regions by the Indian and East Asian monsoons and the southern branch of westerly winds. 3) Soil, water, snow, glacier, sediment, and vegetation act as vital sinks of atmospheric deposits of HMs; 4) Significant bioaccumulation of arsenic (As), lead (Pb), and methylmercury (MeHg) have been found in terrestrial and aquatic biota chains in the TP; 5) The enhancement of anthropogenic activities, climate change, glacial retreat and permafrost degradation had potential impacts on the behaviors and fates of HMs in the TP. Therefore, the ecological risk of HMs is of particular concern, and feasible and effective environmental safety strategies are required to reduce the adverse effects of inorganic pollutants in the TP. Our review will provide a reference for researchers to further study regional HMs pollution around the TP.
Interaction Between Construction Land Expansion and Cropland Expansion and Its Socioeconomic Determinants: Evidence From Urban Agglomeration in the Middle Reaches of the Yangtze River, China
Authors: Wang Liye , Zhang Siyu , Liu Yanfang , Liu Yaolin
Published: April 2022

Nowadays, both urbanization and cropland expansion are hot issues. However, research related to the spatiotemporal interaction between urbanization and cropland expansion and their socioeconomic determinants remains scarce. Accordingly, this research takes the urban agglomeration in the middle reaches of the Yangtze River (MRUA) as the research area by combining spatial analysis, sensitivity analysis, and the spatial gravity model. To achieve this goal, we identified the area of the construction land expansion and cropland expansion, the sensitivity of cropland expansion to construction land expansion, and the shifting trajectory of gravity centers of construction land expansion and cropland expansion and their interaction during 2000–2020, respectively. Additionally, the geographically weighted regression model was utilized to explore the spatiotemporal heterogeneity of four socioeconomic determinants of the interaction between construction land expansion and cropland expansion. The results are as follows: 1) the area of the expanded construction land and the expanded cropland and the sensitivity of cropland expansion to construction land expansion show an overall increasing pattern; 2) the gravity center of the expanded construction land shifted toward the northeast, whereas that of the expanded cropland moved to the southeast but with similar moving distances (17.83 and 15.37 km, respectively); 3) the GDP has an increasing positive effect on the interaction of the construction land expansion and cropland expansion, whereas the investment in fixed assets shows an increasing negative effect during 2000–2020. The GDP in the agricultural sector and population displays a stable influence. This article offers a solution for decision makers to promote the interaction between construction land and cropland.
Colonization During Colonialism: Developing a Framework to Assess the Rapid Ecological Transformation of Mauritius’s Pristine Ecosystem
Authors: Seetah Krish , Manfio Stefania , Balbo Andrea , Farr R. Helen , Florens F. B. Vincent
Published: April 2022

The colonization of Mauritius exemplifies the role played by humans in altering the ecosystems of remote oceanic islands. This paper focuses on how we study those islands first colonized under the global mantle of colonialism. Here we aim to provide a framework for historical ecological investigations to disentangle the processes, impacts, and outcomes of colonization during colonialism, considering local, regional, and global drivers. The paper provides a review of existing literature, outlines a proposed research program encompassing paleoecology, paleoclimatology, archeology, and history, and offers details of potential research sites. We present “historical ecology” as a framework to aid future work, and argue that a refined understanding of the impact of human colonization can help create a nuanced chronology of environmental degradation that typifies Mauritius. Such detailed assessment is necessary to inform contemporary ecological conservation efforts. Finally, we argue that narratives of changing ecosystems and practice can help construct “usable pasts,” often missing from historical records, for the multicultural populace of the island.
Introgressing cry1Ac for Pod Borer Resistance in Chickpea Through Marker-Assisted Backcross Breeding
Authors: Kaur Ajinder , Sharma Urvashi , Singh Sarvjeet , Singh Ravinder , Vikal Yogesh , Singh Satnam , Malik Palvi , Kaur Khushpreet , Singh Inderjit , Bindra Shayla , Sarmah Bidyut Kumar , Sandhu Jagdeep Singh
Published: April 2022

The gram pod borer Helicoverpa armigera is a major constraint to chickpea (Cicer arietinum L.) production worldwide, reducing crop yield by up to 90%. The constraint is difficult to overcome as chickpea germplasm including wild species either lacks pod borer resistance or if possessing resistance is cross-incompatible. This study describes conversion of elite but pod borer-susceptible commercial chickpea cultivars into resistant cultivars through introgression of cry1Ac using marker-assisted backcross breeding. The chickpea cultivars (PBG7 and L552) were crossed with pod borer-resistant transgenic lines (BS 100B and BS 100E) carrying cry1Ac that led to the development of BC1F1, BC1F2, BC1F3, BC2F1, BC2F2, and BC2F3 populations from three cross combinations. The foreground selection revealed that 35.38% BC1F1 and 8.4% BC1F2 plants obtained from Cross A (PBG7 × BS 100B), 50% BC1F1 and 76.5% BC1F2 plants from Cross B (L552 × BS 100E), and 12.05% BC2F2 and 82.81% (average) BC2F3 plants derived from Cross C (PBG7 × BS 100E) carried the cry1Ac gene. The bioassay of backcross populations for toxicity to H. armigera displayed up to 100% larval mortality. BC1F1 and BC1F2 populations derived from Cross B and BC2F3 population from Cross C segregated in the Mendelian ratio for cry1Ac confirmed inheritance of a single copy of transgene, whereas BC1F1 and BC1F2 populations obtained from Cross A and BC2F2 population from Cross C exhibited distorted segregation ratios. BC1F1 plants of Cross A and Cross B accumulated Cry1Ac protein ranging from 11.03 to 11.71 µgg−1 in leaf tissue. Cry1Ac-positive BC2F2 plants from Cross C demonstrated high recurrent parent genome recovery (91.3%) through background selection using SSR markers and phenome recovery of 90.94%, amongst these 30% plants, were homozygous for transgene. The performance of BC2F3 progenies derived from homozygous plants was similar to that of the recurrent parent for main agronomic traits, such as number of pods and seed yield per plant. These progenies are a valuable source for H. armigera resistance in chickpea breeding programs.