bluuebunny/crossref_metadata_2025 huggingface dataset:
Tryptophan intake, not always the more the better
Authors: Hu Dongmei , Liu Junyi , Yu Wanlin , Li Chuan , Huang Lihua , Mao Wei , Lu Zhaoyu
Published: April 2023

Objectives

To investigate the effects of excessive tryptophan intake on the body and the effects of tryptophan metabolism-related aryl hydrocarbon receptor (AhR) pathway in healthy rats and chronic kidney disease rats, to study the adverse effects of excess tryptophan.

Design

In Part I Experiment, the healthy rats were fed with diet containing 0.6, 1.2 and 1.8% tryptophan for 12 weeks. After the intervention, the blood and kidney tissues were collected. Serum creatinine and blood urea nitrogen were detected. Hematoxylin–eosin (H&E) staining was used to observe renal pathological changes. Enzyme-linked immunosorbent assay was used to detect serum kynurenic acid and AhR levels. The kidney levels of AhR, CyP1A1 and CyP1B1 were detected by western-blot. In Part II Experiment, the chronic kidney disease (CKD) model was induced by intra-gastric gavage with adenine for 4 weeks. Then the CKD rats were given tryptophan at a dose of 100 mg/kg or 500 mg/kg for eight weeks. Rat survival curve, renal function, renal tissue pathology and serum AhR were detected. Tryptophan-targeted ultra-high-performance liquid chromatography coupled with multiple reaction monitoring mass spectrometry (UHPLC-MRM-MS) was employed to quantitatively access the tryptophan-targeted metabolites in two parts experiments.

Results

In part I experiment, high tryptophan diet can increase the level of blood urea nitrogen (BUN) in healthy rats and induce focal renal tubulointerstitial injury. Tryptophan-targeted analyzes showed that high tryptophan diet feeding can significantly increase the concentration of kynurenine and indole metabolites. The serum AhR level and kidney AhR, CyP1A1 and CyP1B1 were also significantly increased in high tryptophan diet rats. In part II experiment, high tryptophan intervention induced a significant increase in mortality, serum creatinine, urea nitrogen levels, and renal pathological damage in CKD rats. The levels of tryptophan-targeted metabolites, kynurenine, xanthurenate, picolinic acid, 5-hydroxyindole-3-acetic acid, indole-3-lactic acid, indoleacetate and indoxyl sulfate, showed an upward trend in the high-dose tryptophan group (Ade + Trp-H) compared with the adenine group. The serum AhR of Ade + Trp-H rats was significantly higher than those of adenine rats.

Conclusion

Moderate tryptophan intake may be beneficial, but excessive tryptophan can lead to accumulation of kynurenine and indole metabolites, activate AhR pathway and induce kidney injury.
A new paradigm for epidermal growth factor receptor expression exists in PTC and NIFTP regulated by microRNAs
Authors: Al-Abdallah Abeer , Jahanbani Iman , Ali Rola H. , Al-Brahim Nabeel , Prasanth Jeena , Al-Shammary Bashayer , Al-Bader Maie
Published: April 2023

Intoduction

Identification of molecular alterations associated with tumor behavior is necessary to guide clinical management. The 2022 WHO classification has organized the thyroid follicular cell-derived neoplasms into benign, low-risk and high-risk neoplasms, and emphasized the value of biomarkers that may provide differential diagnostic and prognostic information to avoid overtreatment of low risk neoplasms. This work aims to study the epidermal growth factor receptor (EGFR) expression, functional and spatial dynamics in relation to specific miRNAs alterations in papillary thyroid cancer (PTC) and in non-invasive follicular thyroid neoplasm with papillary-like nuclear features (NIFTP) considered as models of high-risk and low-risk thyroid tumors respectively.

Methods

Primary thyroid cultured cells were used for miRNA gain/loss of function and luciferase reporter assays. Paraffin embedded tissues were used for real time PCR, immuno-fluorescence stain and confocal microscopy experiments.

Results

Our results showed that in PTC, EGFR mRNA is reduced as an effect of miR-146b-5p upregulation. The EGF expression is low and the ERK pathway is inhibited. The EGFR protein high cytoplasmic expression and colocalization with the endosomal/exosomal markers, ALIX and CD63, suggest the occurrence of stress-induced EGFR internalization, accumulation in endosomal vesicles and secretion via exosomes. In NIFTP EGFR transcription is increased in association with downregulation of miR-7-5p and the EGFR/ERK pathway is active indicating dependence on the canonical EGFR pathway for growth.

Conclusion

Downregulation of transcript level along with cytoplasmic accumulation of undegraded protein is a new pattern of EGFR regulation associated with malignancy in thyroid. Further research is needed to elucidate the intracellular trafficking defects responsible for this specific EGFR dynamic in PTC.
Case report: High-risk acute promyelocytic leukemia and COVID-19-related myocarditis one patient, two cytokine storms
Authors: Ghiaur Alexandra , Doran Cristina , Ionescu Bogdan , Mohorea-Neata Lacramioara –Andreea , Stancioaica Camelia , Hirjan Roxana , Tatic Aurelia , Cirstea Mihaela , Vasilache Didona , Tabac Dana , Lupescu Ioana , Coriu Daniel
Published: April 2023

Acute promyelocytic leukemia (APL) is a unique, highly curable subtype of acute myeloid leukemia, owing to the therapeutic advances of the last decades which led to high complete remission rates and excellent long-term survival. Nevertheless, it remains associated with high early mortality rates. Early death is the major cause of treatment failure in APL and is mainly attributed to coagulopathy, differentiation syndrome, and less commonly, infectious events. Timely recognition of each complication plays a crucial role in the management of patients diagnosed with APL. Coronavirus Infectious Disease 2019 (COVID-19) has shown great heterogeneity in patient presentation. Clinical manifestations range from asymptomatic disease to severe forms, mainly characterized by a hyperinflammatory syndrome leading to acute respiratory distress and multiorgan failure. Patients with acute leukemia and concomitant COVID-19-related hyperinflammatory syndrome have particularly poor outcomes. We hereby report the case of a 28-year-old male patient who was diagnosed with high-risk APL, with severe associated coagulopathy at presentation. He was treated with chemotherapy according to the AIDA regimen. The first week of induction therapy was complicated by a differentiation syndrome manifesting as fever not attributable to infection and respiratory distress with pulmonary infiltrates, resolved after ATRA discontinuation and corticotherapy. On the fourth week of treatment, he tested positive for acute respiratory syndrome coronavirus 2 (SARS-CoV-2) with minor pulmonary involvement. Clinical manifestations over the following days included tachycardia and hypotension, associated with elevated inflammatory markers and cardiac biomarkers (troponin I x58 upper NV). Cardiovascular magnetic resonance imaging was consistent with myocarditis. COVID-19-associated myocarditis was successfully treated with methylprednisolone, intravenous immunoglobulins and Anakinra. Differentiation syndrome and COVID-19-associated myocarditis are two life-threatening complications that adversely impact survival. However, early recognition and prompt treatment initiation can improve clinical outcomes, as was the case of our patient.
Treatment options for recurrent platinum-resistant ovarian cancer: A systematic review and Bayesian network meta-analysis based on RCTs
Authors: Li Juan , Zou Guorong , Wang Wei , Yin Chen , Yan Haowen , Liu Shengpeng
Published: April 2023

Background

There are a variety of treatment options for recurrent platinum-resistant ovarian cancer, and the optimal specific treatment still remains to be determined. Therefore, this Bayesian network meta-analysis was conducted to investigate the optimal treatment options for recurrent platinum-resistant ovarian cancer.

Methods

Pubmed, Cochrane, Embase, and Web of Science were searched for articles published until 15 June 2022. The outcome measures for this meta-analysis were overall survival (OS), progression-free survival (PFS), and adverse events (AEs) of Grade 3-4. The Cochrane assessment tool for risk of bias was used to evaluate the risk of bias of the included original studies. The Bayesian network meta-analysis was conducted. This study was registered on PROSPERO (CRD42022347273).

Results

Our systematic review included 11 RCTs involving 1871 patients and 11 treatments other than chemotherapy. The results of meta-analysis showed that the overall survival (OS) was the highest in adavosertib + gemcitabine compared with conventional chemotherapy, (HR=0.56,95%CI:0.35-0.91), followed by sorafenib + topotecan (HR=0.65, 95%CI:0.45-0.93). In addition, Adavosertib + Gemcitabine regimen had the highest PFS (HR=0.55,95%CI:0.34-0.88), followed by Bevacizumab + Gemcitabine regimen (HR=0.48,95%CI:0.38-0.60) and the immunotherapy of nivolumab was the safest (HR=0.164,95%CI:0.312-0.871) with least adverse events of Grades 3-4.

Conclusions

The results of this study indicated that Adavosertib (WEE1 kinase-inhibitor) + gemcitabine regimen and Bevacizumab + Gemcitabine regimen would be significantly beneficial to patients with recurrent platinum-resistant ovarian cancer, and could be preferred for recurrent platinum-resistant ovarian cancer. The immunotherapeutic agent, Nivolumab, is of considerable safety, with a low risk for grade-III or IV adverse events. Its safety is comparable to Adavosertib + gemcitabine regimen. Pazopanib + Paclitaxel (weekly regimen), Sorafenib + Topotecan/Nivolumab could be selected if there are contraindications of the above strategies.

Systematic review registration

https://www.crd.york.ac.uk/prospero/, identifier CRD42022347273.
Clinicopathological and survival outcomes of 4L lymph node dissection in left lung adenocarcinoma and squamous cell carcinoma
Authors: Shen Leilei , Guo Juntang , Zhang Weidong , Liang Chaoyang , Chen Han , Liu Yang
Published: April 2023

Background

Whether 4L lymph node dissection (LND) should be performed remains unclear and controversial. Prior studies have found that station 4L metastasis was not rare and that 4L LND may provide survival benefits. The objective of this study was to analyze the clinicopathological and survival outcomes of 4L LND from the perspective of histology.

Methods

This retrospective study included 74 patients with squamous cell carcinoma (SCC) and 84 patients diagnosed with lung adenocarcinoma (ADC) between January 2008 and October 2020. All patients underwent pulmonary resection with station 4L LND and were staged as T1-4N0-2M0. Clinicopathological features and survival outcomes were investigated based on histology. The study endpoints were disease-free survival (DFS) and overall survival (OS).

Results

The incidence rate of station 4L metastasis was 17.1% (27/158) in the entire cohort, with 8.1% in the SCC group, and 25.0% in the ADC group. No statistical differences in the 5-year DFS rates (67.1% vs. 61.7%, P=0.812) and 5-year OS rates (68.6% vs. 59.3%, P=0.100) were observed between the ADC group and the SCC group. Multivariate logistic analysis revealed that histology (SCC vs. ADC: OR, 0.185; 95% CI, 0.049–0.706; P=0.013) was independently associated with 4L metastasis. Multivariate survival analysis showed that the status of 4L metastasis was an independent factor for DFS (HR, 2.563; 95% CI, 1.282–5.123; P=0.008) but not for OS (HR, 1.597; 95% CI, 0.749–3.402; P=0.225).

Conclusion

Station 4L metastasis is not rare in left lung cancer. Patients with ADC have a greater predilection for station 4L metastasis and may benefit more from performing 4L LND.
Diagnostic value of combination of biomarkers for malignant pleural mesothelioma: a systematic review and meta-analysis
Authors: Zhu Mucheng , Lu Zhenhua , Guo Hao , Gu Xiaoting , Wei Defang , Zhang Zhengyi
Published: April 2023

Introduction

Early-stage accurate diagnosis of malignant pleural mesothelioma (MPM) has always been a formidable challenge. DNA and protein as biomarkers for the diagnosis of MPM have received considerable attention, and yet the outcomes are inconsistent.

Methods

In this study, a systematic search employing PubMed, EMBASE, and Cochrane Library to identify relevant studies from the first day of databases to October 2021. Moreover, we adopt the QUADAS-2 to evaluate the quality of eligible studies and Stata 15.0 and Review Manager 5.4 software programs to perform the meta-analysis. Additionally, bioinformatics analysis was performed at GEPIA for the purpose of exploring relationship between related genes and the survival time of MPM patients.

Results

We included 15 studies at the DNA level and 31studies at the protein level in this meta-analysis. All results demonstrated that the diagnostic accuracy of the combination of MTAP + Fibulin-3 was the highest with the SEN 0.81 (95% CI: 0.67, 0.89) and the SPE 0.95 (95% CI: 0.90, 0.97). And the bioinformatics analysis indicated that the higher MTAP gene expression level was beneficial to enhance the survival time of MPM patients.

Discussion

Nonetheless, as a result of the limitations of the included samples, it may be necessary to conduct additional research before drawing conclusions.

Systematic review registration

https://inplasy.com/inplasy-2022-10-0043/, identifier INPLASY2022100043.
Primary ectopic meningioma in the thoracic cavity: A rare case report and review of the literature
Authors: Liu Xu , Liu Jiao , Nai Ting , Yang Yuxia , Hu Yuchang
Published: April 2023

Background

Meningioma is a common type of intracranial tumor in adults. It rarely arises in the chest, with only a few case reports in the English literature. Here, we report the case of a patient with a primary ectopic meningioma (PEM) located in the thoracic cavity.

Case presentation

A 55-year-old woman presented with exercise-induced asthma, chest tightness, intermittent dry cough and fatigue for several months. Computed tomography revealed the presence of a huge mass in the thoracic cavity, with no connection to the spinal canal. Lung cancer and mesothelioma were suspected, and surgery was performed. Grossly, the mass was a grayish-white solid 9.5 cm × 8.4 cm × 5.3 cm in size. The microscopic morphology of the lesion was consistent with that of typical central nervous system meningioma. The pathological subtype was transitional meningioma. The tumor cells were arranged in a fascicular, whorled, storiform and meningithelial pattern, with occasional intranuclear pseudo-inclusions and psammoma bodies. In focal areas tumor cells were considerably dense, and the cells were round or irregular in shape, with less cytoplasm, uniform nuclear chromatin, and visible nucleoli and mitoses (2/10 HPF). By immunohistochemistry, the neoplastic cells showed strong and diffuse staining with vimentin, epithelial membrane antigen and SSTR2 with variable expression of PR, ALK and S100 protein. However, the cells were negative for GFAP, SOX-10, inhibin, CD34, STAT6, smooth muscle actin, desmin, CKpan, D2-40, WT-1, CK5/6 and CD45. The highest proliferation index by Ki-67 was 15%. The abnormal expression of ALK led to the initial misdiagnosis of an inflammatory myofibroblastic tumor. After 12 months of follow-up, no disease progression was observed.

Conclusion

The presence of primary ectopic meningiomas in the thoracic cavity is extremely rare, and this tumor is easily misdiagnosed clinically. Imaging is suggested to determine the location and possible differential diagnosis, while the final diagnosis should be via pathological examination. Immunohistochemistry is crucial for disease diagnosis. Owing to our limited knowledge of PEM, its pathogenesis and tissue of origin remain unclear. Clinicians should pay close attention to such potential patients. The present case report may provide insights into the diagnosis and therapy of patients with this tumor.
Joint statement for assessing and managing high blood pressure in children and adolescents: Chapter 1. How to correctly measure blood pressure in children and adolescents
Authors: Lurbe Empar , Mancia Giuseppe , Calpe Javier , Drożdż Dorota , Erdine Serap , Fernandez-Aranda Fernando , Hadjipanayis Adamos , Hoyer Peter F. , Jankauskiene Augustina , Jiménez-Murcia Susana , Litwin Mieczysław , Mazur Artur , Pall Denes , Seeman Tomas , Sinha Manish D. , Simonetti Giacomo , Stabouli Stella , Wühl Elke
Published: April 2023

The joint statement is a synergistic action between HyperChildNET and the European Academy of Pediatrics about the diagnosis and management of hypertension in youth, based on the European Society of Hypertension Guidelines published in 2016 with the aim to improve its implementation. The first and most important requirement for the diagnosis and management of hypertension is an accurate measurement of office blood pressure that is currently recommended for screening, diagnosis, and management of high blood pressure in children and adolescents. Blood pressure levels should be screened in all children starting from the age of 3 years. In those children with risk factors for high blood pressure, it should be measured at each medical visit and may start before the age of 3 years. Twenty-four-hour ambulatory blood pressure monitoring is increasingly recognized as an important source of information as it can detect alterations in circadian and short-term blood pressure variations and identify specific phenotypes such as nocturnal hypertension or non-dipping pattern, morning blood pressure surge, white coat and masked hypertension with prognostic significance. At present, home BP measurements are generally regarded as useful and complementary to office and 24-h ambulatory blood pressure for the evaluation of the effectiveness and safety of antihypertensive treatment and furthermore remains more accessible in primary care than 24-h ambulatory blood pressure. A grading system of the clinical evidence is included.
Error Analysis for Repeatability Enhancement of a Dual-Rotation Mueller Matrix Ellipsometer
Authors: Jiang Zhou , Zhang Song , Liu Jiaming , Li Qi , Jiang Hao , Liu Shiyuan
Published: April 2023

Since the Mueller matrix ellipsometer has been used as a highly accurate tool for thin film measurement, the error analysis and repeatability enhancement of such a tool are of great importance. The existence of the Poisson–Gaussian mixed noise and the random bias of the trigger signal in the optical measurement system may reduce the repeatability and accuracy of a measurement. Utilizing the probabilistic analysis, the random errors in the Mueller matrix measurements are quantified. A quantitative analysis on the instrument matrix has been carried out to assess the individual effects for different error sources. We proposed a general optimal instrument matrix which is capable of minimizing the estimation variance for both Gaussian additive noise and Poisson shot noise. Besides, a peak-matching algorithm is proposed to compress the repeatability errors due to the bias of the trigger signal and the limited sampling frequency. The effectiveness of the proposed methods is shown using both virtual simulations and experiments carried out on our self-developed instrument, which potentially paves a way to reduce the requirements on motor performance, acquisition card resolution, and trigger accuracy, which are critical to cost reduction.
Fractional solitons: New phenomena and exact solutions
Authors: Zeng Huajun , Wang Yuxia , Xiao Min , Wang Ying
Published: April 2023

The fractional solitons have demonstrated many new phenomena, which cannot be explained by the traditional solitary wave theory. This paper studies some famous fractional wave equations including the fractional KdV–Burgers equation and the fractional approximate long water wave equation by a modified tanh-function method. The solving process is given in details, and new solitons can be rigorously explained by the obtained exact solutions. This paper offers a new window for studying fractional solitons.
Dietary ketone ester attenuates the accretion of adiposity and liver steatosis in mice fed a high-fat, high-sugar diet
Authors: Rushing Kelsey A. , Bolyard Mickey L. , Kelty Taylor , Wieschhaus Nicole , Pavela Gregory , Rector R. Scott , Plaisance Eric P.
Published: April 2023

Objective: The ketone diester, R,S-1,3-butanediol diacetoacetate (BD-AcAc2), attenuates the accretion of adiposity and reduces hepatic steatosis in high-fat diet-induced obese mice when carbohydrate energy is removed from the diet to accommodate energy from the ester. Reducing carbohydrate energy is a potential confounder due to the well-known effects of carbohydrate restriction on components of energy balance and metabolism. Therefore, the current investigation was designed to determine whether the addition of BD-AcAc2 to a high-fat, high-sugar diet (with no reduction in carbohydrate energy) would attenuate the accretion of adiposity and markers of hepatic steatosis and inflammation.

Methods: Sixteen 11-week-old male C57BL/6J mice were randomized to one of two groups for 9 weeks (n = 8 per group): 1) Control (CON, HFHS diet) or 2) Ketone ester (KE, HFHS diet + BD-AcAc2, 25% by kcals).

Results: Body weight increased by 56% in CON (27.8 ± 2.5 to 43.4 ± 3.7 g, p < 0.001) and by 13% in KE (28.0 ± 0.8 to 31.7 ± 3.1 g, p = 0.001). Non-alcoholic fatty liver disease activity scores (NAS) for hepatic steatosis, inflammation, and ballooning were lower in the KE group compared to CON (p < 0.001 for all). Markers of hepatic inflammation [Tnfα (p = 0.036); Mcp1 (p < 0.001)], macrophage content [(Cd68 (p = 0.012)], and collagen deposition and hepatic stellate cell activation [(αSma (p = 0.004); Col1A1 (p < 0.001)] were significantly lower in the KE group compared to CON.

Conclusion: These findings extend those of our previous work and show that BD-AcAc2 attenuates the accretion of adiposity and reduces markers of liver steatosis, inflammation, ballooning, and fibrosis in lean mice placed on a HFHS diet where carbohydrate energy was not removed to accommodate energy from addition of the diester.
A novel transfer learning framework for sorghum biomass prediction using UAV-based remote sensing data and genetic markers
Authors: Wang Taojun , Crawford Melba M. , Tuinstra Mitchell R.
Published: April 2023

Yield for biofuel crops is measured in terms of biomass, so measurements throughout the growing season are crucial in breeding programs, yet traditionally time- and labor-consuming since they involve destructive sampling. Modern remote sensing platforms, such as unmanned aerial vehicles (UAVs), can carry multiple sensors and collect numerous phenotypic traits with efficient, non-invasive field surveys. However, modeling the complex relationships between the observed phenotypic traits and biomass remains a challenging task, as the ground reference data are very limited for each genotype in the breeding experiment. In this study, a Long Short-Term Memory (LSTM) based Recurrent Neural Network (RNN) model is proposed for sorghum biomass prediction. The architecture is designed to exploit the time series remote sensing and weather data, as well as static genotypic information. As a large number of features have been derived from the remote sensing data, feature importance analysis is conducted to identify and remove redundant features. A strategy to extract representative information from high-dimensional genetic markers is proposed. To enhance generalization and minimize the need for ground reference data, transfer learning strategies are proposed for selecting the most informative training samples from the target domain. Consequently, a pre-trained model can be refined with limited training samples. Field experiments were conducted over a sorghum breeding trial planted in multiple years with more than 600 testcross hybrids. The results show that the proposed LSTM-based RNN model can achieve high accuracies for single year prediction. Further, with the proposed transfer learning strategies, a pre-trained model can be refined with limited training samples from the target domain and predict biomass with an accuracy comparable to that from a trained-from-scratch model for both multiple experiments within a given year and across multiple years.
Functional divergence of Heat Shock Factors (Hsfs) during heat stress and recovery at the tissue and developmental scales in C4 grain amaranth (Amaranthus hypochondriacus)
Authors: Goel Komal , Kundu Pravesh , Gahlaut Vijay , Sharma Paras , Kumar Ayush , Thakur Shiwali , Verma Vipasha , Bhargava Bhavya , Chandora Rahul , Zinta Gaurav
Published: April 2023

Two major future challenges are an increase in global earth temperature and a growing world population, which threaten agricultural productivity and nutritional food security. Underutilized crops have the potential to become future climate crops due to their high climate-resilience and nutritional quality. In this context, C4 pseudocereals such as grain amaranths are very important as C4 crops are more heat tolerant than C3 crops. However, the thermal sensitivity of grain amaranths remains unexplored. Here, Amaranthus hypochondriacus was exposed to heat stress at the vegetative and reproductive stages to capture heat stress and recovery responses. Heat Shock Factors (Hsfs) form the central module to impart heat tolerance, thus we sought to identify and characterize Hsf genes. Chlorophyll content and chlorophyll fluorescence (Fv/Fm) reduced significantly during heat stress, while malondialdehyde (MDA) content increased, suggesting that heat exposure caused stress in the plants. The genome-wide analysis led to the identification of thirteen AhHsfs, which were classified into A, B and C classes. Gene expression profiling at the tissue and developmental scales resolution under heat stress revealed the transient upregulation of most of the Hsfs in the leaf and inflorescence tissues, which reverted back to control levels at the recovery time point. However, a few Hsfs somewhat sustained their upregulation during recovery phase. The study reported the identification, physical location, gene/motif structure, promoter analysis and phylogenetic relationships of Hsfs in Amaranthus hypochondriacus. Also, the genes identified may be crucial for future gene functional studies and develop thermotolerant cultivars.
Hostage negotiator resilience: A phenomenological study of awe
Authors: Thompson Jeff , Jensen Elizabeth
Published: April 2023

Law enforcement crisis and hostage negotiators (CHNs) are tasked with resolving incidents that are stressful, unpredictable, and often dangerous. These negotiators must work as a team and be able to successfully utilize a variety of skills in order to gain the subject’s voluntary compliance and peaceful surrender. It is necessary for negotiators to continually practice these skills while also, and importantly, care for their own wellbeing. This study examines how a phenomenon, awe, when viewed as a resilience practice, can support law enforcement crisis hostage negotiators with their crisis work and personal wellness. Utilizing phenomenological methodologies, the findings demonstrate that reflecting on awe experiences had an overall positive impact on the negotiators in both their professional and personal lives. Based on the results, it is suggested that awe practices could be incorporated into future negotiator trainings in order to increase resilience and assist negotiators personally and professionally.
Developing the Metacognitive Awareness of Grit Scale for a better understanding of learners of English as a foreign language
Authors: Wang Mingzhe , Zhang Lawrence Jun , Hamilton Richard
Published: April 2023

The prominent impact of metacognition on learners’ academic achievement is widely discussed. Learners armed with appropriate metacognitive strategies should witness enhancement in learning performance. Similarly, the concept of grit is also valued as a crucial factor contributing to the improvement of academic achievement. Nevertheless, discussion of the relationship between metacognition and grit or their collective influence on other educational and psychological variables is limited, not to mention that an instrument measuring learners’ metacognitive awareness of grit is a desideratum. Hence, by incorporating the constructs of metacognition and grit, the present research developed a measurement scale to address this need, named the Metacognitive Awareness of Grit Scale (MCAGS). The MCAGS consists of four components and initially included 48 items. It was later distributed to 859 participants for the purpose of scale validation. Confirmatory factor analysis was applied to evaluate the scale’s validity and explore the factor-item relationship. A final model containing 17 items was retained. Implications and future directions were discussed.
Inequality and barriers in psychosis prevention: A systematic review on clinical high-risk for psychosis studies from developing countries
Authors: Loch Alexandre Andrade , Lopes-Rocha Ana Caroline , Fekih-Romdhane Feten , van de Bilt Martinus Theodorus , Salazar de Pablo Gonzalo , Fusar-Poli Paolo
Published: April 2023

Background

The clinical high-risk for psychosis (CHR) paradigm is one of the best studied preventive paradigms in psychiatry. However, most studies have been conducted in high-income countries. It is unclear if knowledge from such countries applies to low and middle-income countries (LAMIC), and if there are specific limitations hindering CHR research there. Our aim is to systematically review studies on CHR from LAMIC.

Methods

A multistep PRISMA-compliant literature search was performed in PubMed and Web of Science for articles published until 1/03/2022, conducted in LAMIC, addressing the concept and correlates of CHR. Study characteristics as well as limitations were reported. Corresponding authors of the included studies were invited to answer an online poll. Quality assessment was done with the MMAT.

Results

A total of 109 studies were included in the review: none from low-income countries, 8 from lower middle-income countries, and 101 from upper middle-income countries. The most frequent limitations were small sample size (47.9%), cross-sectional design (27.1%), and follow-up issues (20.8%). Mean quality of included studies was of 4.4. Out of the 43 corresponding authors, 12 (27.9%) completed the online poll. They cited further limitations as few financial resources (66.7%), no involvement of population (58.2%) and cultural barriers (41.7%). Seventy five percent researchers reported that CHR research should be conducted differently in LAMIC compared to high-income countries, due to structural and cultural issues. Stigma was mentioned in three out of five sections of the poll.

Discussion

Results show the discrepancy of available evidence on CHR in LAMIC, given the shortage of resources in such countries. Future directions should aim to increase the knowledge on individuals at CHR in such settings, and to address stigma and cultural factors that may play a role in the pathways toward care in psychosis.

Systematic Review Registration

https://www.crd.york.ac.uk/prospero/display_record.php?RecordID=316816, CRD42022316816.
Dismantling weight stigma in eating disorder treatment: Next steps for the field
Authors: McEntee Mindy L. , Philip Samantha R. , Phelan Sean M.
Published: April 2023

The authors posit current guidelines and treatment for eating disorders (EDs) fail to adequately address, and often perpetuate, weight stigma. The social devaluation and denigration of higher-weight individuals cuts across nearly every life domain and is associated with negative physiological and psychosocial outcomes, mirroring the harms attributed to weight itself. Maintaining focus on weight in ED treatment can intensify weight stigma among patients and providers, leading to increased internalization, shame, and poorer health outcomes. Stigma has been recognized as a fundamental cause of health inequities. With no clear evidence that the proposed mechanisms of ED treatment effectively address internalized weight bias and its association with disordered eating behavior, it is not hard to imagine that providers’ perpetuation of weight bias, however unintentional, may be a key contributor to the suboptimal response to ED treatment. Several reported examples of weight stigma in ED treatment are discussed to illustrate the pervasiveness and insidiousness of this problem. The authors contend weight management inherently perpetuates weight stigma and outline steps for researchers and providers to promote weight-inclusive care (targeting health behavior change rather than weight itself) as an alternative approach capable of addressing some of the many social injustices in the history of this field.
Type D personality to insomnia: Sleep reactivity, sleep effort, and sleep hygiene as mediators
Authors: Uygur Omer Faruk , Ahmed Oli , Uygur Hilal , Bahar Aynur , Hursitoglu Onur , Chung Seockhoon , Drake Christopher L.
Published: April 2023

Background

Insomniacs are heterogenous group with very diverse personalities. We aimed to investigate the mediating role of sleep reactivity (SR), sleep hygiene (SH), and sleep effort (SE) in the relationship between Type D personality and insomnia.

Materials and methods

We conducted a cross-sectional survey among 474 participants. The survey comprised the sociodemographic data form, Insomnia Severity Index (ISI), D Type Personality Scale (DS-14), Ford Insomnia Response to Stress Test (FIRST), Glasgow Sleep Effort Scale (GSES), and Sleep Hygiene Index (SHI). We conducted hierarchical multiple regression analysis to identify the associations between age, sex, SR, Type D personality traits, SE, SH, and insomnia severity. We subsequently conducted mediation analyses to examine whether SR, SH, and SE mediated the relationship between Type D personality and insomnia.

Results

ISI, DS-14, FIRST, SHI, and GSES scores were significantly higher in individuals with Type D personality. Female sex, SR, Type D personality traits, SE, and SH explained 45% of the variance in insomnia severity. When age, sex, insomnia response to stress, and Type D personality traits were controlled, SE and SH significantly explained 25% of the variance in insomnia severity (R2 = 0.45, R2 change = 0.25, F (6.474) = 65.58, p < 0.001). SR, SE, and SH each played a partial mediating role between Type D personality and insomnia.

Conclusion

The findings showed that individuals with Type D personality had high SR and that individuals with a higher number of these personality traits exhibited more severe insomnia symptoms through high SR, greater SE, and worse SH.
Replacing Animal Testing: How and When?
Authors: Hartung Thomas
Published: April 2023

An important discussion in today’s society is whether we should make animals suffer for the sake of science and product development. In this article, I present four examples of animal tests that were introduced in the past to protect patients and consumers, and I discuss attempts to replace those animal tests with other methods. When we started using small animals such as mice and rats for testing more than 100 years ago, there were not many alternatives. Today, we have more knowledge and a greater number of options. Scientists can now create tiny functioning organs in the laboratory, and even combine multiple mini-organs, to help us understand how the human body works when it is healthy or sick. This increased understanding will allow scientists to move beyond the use of animals in many cases, which will improve both the accuracy of the scientific tests and the welfare of animals.
Empowering rural service providers to improve nutrition in mountain regions
Authors: Sabir Ghezal , Bernet Thomas , Riar Amritbir
Published: April 2023

Improved agroecological practices tend to lead to production diversity but often fall short of achieving optimal dietary diversity in rural contexts. Thus, multi-prong interventions involving knowledge and skill development for production and consumption are needed. This study assesses nutrition and production-related interventions implemented in the East African Highlands, Pamir-Tian Shan, Hindukush and the Andes. Overall, 601 rural service providers were trained and supported to implement various nutrition-sensitive agroecological interventions. Study results show that these interventions significantly increased the intake of flesh food, eggs, vitamin A-rich leafy greens, and vitamin-A-rich vegetables and fruits. The increase in production diversity was correlated with the increase in dietary diversity. Thus this study highlights the crucial role of rural service providers in catalyzing nutrition-related changes in mountain contexts.
Management strategy of a carotid-esophageal fistula with a right common carotid artery pseudoaneurysm due to chicken bone ingestion: A case report and literature review
Authors: Xu Dongming , Qian Haichao , Lian Lishan , Xie Miaorong , Zhang Hanyu
Published: April 2023

Common carotid artery (CCA) pseudoaneurysm is a rare clinical disorder. CCA pseudoaneurysm that occurs with a carotid-esophageal fistula and causes massive upper gastrointestinal bleeding is especially uncommon but can be life-threatening. Accurate diagnosis and prompt managements are essential to save lives. Here, we report a case of a 58-year-old female who presented with dysphagia and throat pain after accidental ingestion of a chicken bone. The patient presented with active upper gastrointestinal bleeding which quickly developed into hemorrhage shock. Imaging studies confirmed a diagnosis of right CCA pseudoaneurysm and carotid-esophageal fistula. The patient had a satisfactory recovery after a right CCA balloon occlusion, right CCA pseudoaneurysm excision, and right CCA and esophageal repairs. We present and discuss this case here to remind physicians to rule out rare causes of upper gastrointestinal bleeding. A multidisciplinary approach is commonly required to achieve satisfactory outcomes in these cases.
Effect of replacing whole wheat with broken rye as a sustainable grain in diets of fattening turkeys on growth performance, litter quality, and foot pad health
Authors: Lingens Jan Berend , Visscher Christian , Sürie Christian , Grone Richard , von Felde Andreas , Wilke Volker , Abd El-Wahab Amr
Published: April 2023

Introduction

Rye is one of the most important cereal crops in Central Europe, thus attempts have been made to include it in the diets of birds to reduce production costs, since the cost of feed accounts for as much as 50 %−70 % thereof. Nevertheless, the use of rye has been limited to date, particularly in turkeys. This study aimed to test the effects of rye inclusion up to 10 % on growth, excreta, and/or litter dry matter, and foot pad health.

Methods

Four trials were performed with a total of 4,322, 4,307, 4,256, and 4,280 female turkeys (BUT BIG 6, Aviagen) for trials 1, 2, 3, and 4, respectively. All birds were fed commercial starter diets for the dietary phases 1 and 2 (up to d 35 of life). Thereafter, at the start of the study, the control group received commercial supplementary feed with 5 % or 10 % wheat until the end of the fattening period. The experimental group was offered supplementary feed to which instead of wheat increasing levels of rye were added stepwise from 5 % to 10 %.

Results

Using supplementary feed with rye showed no significant differences in the final body weight between the control and experimental groups (10.9 vs. 10.8 kg). The dry matter content of fresh excreta for turkeys during the experimental period did not show significant differences between both groups, except at weeks 10 and 14 of life. The feed type (either control diet or experimental diet) did not significantly affect litter dry matter content between the groups throughout the experimental period. No significant differences were noted in food pad dermatitis scoring between both groups throughout the experimental period, except at weeks 11 and 16 of life. Overall, this study showed that including proportions of rye up to 10% could replace conventional ingredients and may increase sustainability in poultry production regardless of the addition of supplementary feed.
Strigolactones in Sugarcane Growth and Development
Authors: Zan Fenggang , Wu Zhuandi , Wang Wenzhi , Hu Xin , Feng Lu , Liu Xinlong , Liu Jiayong , Zhao Liping , Wu Caiwen , Zhang Shuzhen , Guo Jiawen
Published: April 2023

Sugarcane is a complex polyploid aneuploid cash crop, and transgenic varieties are important for molecular genetic and traditional breeding approaches. Herein, the sugarcane variety ROC22 served as the receptor, the Bar gene served as a screening marker, and positive and negative fragments of the ScD27.2 gene, upstream of strigolactones (SLs) biosynthesis genes driven by the 35S promoter, were introduced by Agrobacterium tumefaciens-mediated transformation. Regenerated plants were obtained by co-culture, screening culture, and differentiation induction, and 27 sense and antisense ScD27.2 transgenic seedlings were obtained by herbicide screening. PCR detection and 1% Basta (Glufosinate) application on leaves revealed Bar in all lines, with all testing positive for herbicide application and 23 containing the target gene (positive resistance screening rate = 87.5%). q-PCR and phenotypic analyses showed that ScD27.2 expression, plant height, tiller number, root length, stem diameter, and fresh weight were decreased in transgenic (ScD27.2R-9) compared with non-transgenic (NT and ScD27.2F-2) lines. ScD27.2 expression was downregulated, and growth potential was inhibited. Under 20% PEG treatment, malondialdehyde (MDA) content in ScD27.2R-9 was higher than in NT, while proline content was lower. Under drought stress, ScD27.2 expression, MDA levels, and proline content in ScD27.2F-2 and NT were higher than in non-treated controls, ScD27.2 expression increased with time, and MDA and proline levels also increased. ScD27.2 expression in ScD27.2R-9 decreased under 20% PEG treatment, MDA and proline increased (but not to NT levels), and growth was lower than NT. The 20% PEG treatment also increased the levels of (±)-2′-epi-5-deoxystrigol and (+)-abscisic acid in the rooting culture media of ScD27.2F-2, ScD27.2R-9, and NT lines, but the levels of (+)-abscisic acid content in ScD27.2R-9 was lower than in NT. Thus, interfering with ScD27.2 expression decreased resistance to 20% PEG treatment. ScD27.2 encodes a β-carotene isomerase involved in SLs biosynthesis that might function in sugarcane resistance to drought stress. It explains the role of SLs in sugarcane growth and development and responses to drought stress.
Control of Resistant False Cleavers (Galium spurium L.) Population to ALS-Inhibiting Herbicides and Its Impact on the Growth and Yield of Durum Wheat
Authors: Sparangis Panagiotis , Efthimiadou Aspasia , Katsenios Nikolaos , Karkanis Anestis
Published: April 2023

False cleavers (Galium spurium L.) is an annual broadleaf weed, commonly found in cereal crops around the world. It reduces crop yields mainly by the competition for nutrients and plant lodging, which decreases the photosynthetic rate of cultivated plants. Two field experiments were carried out in different locations to examine the efficacy of post-emergence herbicides against false cleavers in durum wheat (Triticum durum Desf.). Herbicides that belong to sulfonylureas, triazolopyrimidines, and other groups (e.g., nitriles and synthetic auxins) were applied. The results revealed that the herbicide florasulam + aminopyralid provided low efficacy (57%) against this weed species, while the most effective herbicides were bromoxynil + 2,4-D and halauxifen-methyl + florasulam. Bromoxynil + 2,4-D efficacy against false cleavers was 73% and 97% at Domokos and Velestino, respectively, while halauxifen-methyl + florasulam efficacy ranged between 89% and 97%. Moreover, the herbicides metsulfuron-methyl + bensulfuron-methyl and pyroxsulam/florasulam + 2,4-D provided low efficacy (<11%) against false cleavers, revealing resistance to ALS-inhibiting herbicides. Regarding the impact of false cleavers and other weed species on the growth of durum wheat, the results showed that the greatest dry biomass (8508.3–8922.7 kg ha−1 and 13,041.4–13,523 kg ha−1 at Domokos and Velestino, respectively) was found in the bromoxynil + 2,4-D, halauxifen-methyl + florasulam, and weed-free treatments. Similar results were also recorded for 1000-seed weights and crop yields, while there were no statistically significant differences among the treatments for spike length. Finally, our results revealed that the herbicides bromoxynil + 2,4-D and halauxifen-methyl + florasulam effectively controlled the resistant false cleavers population. However, it is important to note that halauxifen-methyl + florasulam was recently registered in Greece and other countries and, thus, should be used sensibly by farmers in a rotation with other herbicides to prevent the development of resistant populations.
Fine-Scale Interactions between Leopard Cats and Their Potential Prey with Contrasting Diel Activities in a Livestock-Dominated Nature Reserve
Authors: Ji Chengpeng , Li Hai-Dong , Xiao Wenhong , Xu Kai , Ren Yingfeng , Li Hongyun , Wang Pengcheng , Fan Mingliang , Huang Xiaoqun , Xiao Zhishu
Published: April 2023

Habitat use and the temporal activities of wildlife can be largely modified by livestock encroachment. Therefore, identifying the potential impacts of livestock on the predator–prey interactions could provide essential information for wildlife conservation and management. From May to October 2017, we used camera trapping technology to investigate fine-scale spatiotemporal interactions in a predator–prey system with the leopard cat (Prionailurus bengalensis) as a common mesopredator, and its prey with contrasting activity patterns (i.e., nocturnal rats and diurnal squirrels) in a livestock-dominated nature reserve in Northern China. We found that the prey species showed different habitat preferences with the leopard cats. The nocturnal rats had strong positive effects on the site-use of the leopard cats, while the influence of livestock on the diurnal squirrels’ site-use changed from strong positive effects to weak effects as the livestock disturbance increased. The temporal overlap between the leopard cats and the nocturnal rats was almost four times that of the leopard cats and the diurnal squirrels, regardless of the livestock disturbance. Our study demonstrated that the fine-scale spatiotemporal use patterns of the leopard cats were consistent and highly correlated with the nocturnal rats under livestock disturbance. We suggest that appropriate restrictions on livestock disturbance should be implemented by reserve managers to reduce the threat to wildlife and achieve multi-species coexistence.
Characterization of Bacillus pumilus Strains Isolated from Bovine Uteri
Authors: Ballas Panagiotis , Gabler Christoph , Wagener Karen , Drillich Marc , Ehling-Schulz Monika
Published: April 2023

Uterine infections are a major source of economic losses to dairy farmers. The uterine microbiota as well as opportunistic uterine contaminants can contribute to the development of endometritis in dairy cows during the postpartum period. Therefore, it is important to characterize potential pathogens and to further elucidate their role in the disease. In this study, we aimed to characterize Bacillus pumilus field isolates to obtain more details regarding their effect on uterine cells by using an in vitro endometrial epithelial primary cells model. We found that B. pumilus isolates possessed the keratinase genes ker1 and ker2 and therefore may produce keratinases. When primary endometrial epithelial cells were infected with 4 different B. pumilus strains, an effect on cellular viability was observed over the course of 72 h. The effect was dose-dependent and time-dependent. Nevertheless, significant differences between the strains were not observed. All tested strains reduced the viability of the primary cells after 72 h of incubation, indicating that B. pumilus potentially has a pathogenic effect on endometrial epithelial cells.