bluuebunny/crossref_metadata_2025 huggingface dataset:
Retrieval of Chemical Oxygen Demand through Modified Capsule Network Based on Hyperspectral Data
Authors: Deng Chubo , Zhang Lifu , Cen Yi
Published: October 2019

This study focuses on the retrieval of chemical oxygen demand (COD) in the Baiyangdian area in North China, using a modified capsule network. Herein, the capsule model was modified to analyze the regression relationship between 1-D hyperspectral data and COD values. The results indicate there is a statistically significant correlation between COD and the hyperspectral data. The accuracy of the capsule network was compared with the results obtained from using a traditional back-propagation neural network (BP) method. The capsule network achieved superior accuracy with fewer iterations, compared with the BP algorithm. An R2 value of 0.78 was obtained against measured COD values retrieved using the capsule network method, compared with a value of 0.42 for the BP algorithm retrievals. This suggests the capsule network method has great potential to solve regression problems in the field of remote sensing.
Vectored-Bloom Filter for IP Address Lookup: Algorithm and Hardware Architectures
Authors: Byun Hayoung , Li Qingling , Lim Hyesook
Published: October 2019

The Internet Protocol (IP) address lookup is one of the most challenging tasks for Internet routers, since it requires to perform packet forwarding at wire-speed for tens of millions of incomming packets per second. Efficient IP address lookup algorithms have been widely studied to satisfy this requirement. Among them, Bloom filter-based approach is attractive in providing high performance. This paper proposes a high-speed and flexible architecture based on a vectored-Bloom filter (VBF), which is a space-efficient data structure that can be stored in a fast on-chip memory. An off-chip hash table is infrequently accessed, only when the VBF fails to provide address lookup results. The proposed architecture has been evaluated through both a behavior simulation with C language and a timing simulation with Verilog. The hardware implementation result shows that the proposed architecture can achieve the throughput of 5 million packets per second in a field programmable gate array (FPGA) operated at 100 MHz.
Spatiotemporal Changes of Urban Rainstorm-Related Micro-Blogging Activities in Response to Rainstorms: A Case Study in Beijing, China
Authors: Wang Nan , Du Yunyan , Liang Fuyuan , Yi Jiawei , Wang Huimeng
Published: October 2019

Natural disasters cause significant casualties and losses in urban areas every year. Further, the frequency and intensity of natural disasters have increased significantly over the past couple of decades in the context of global climate change. Understanding how urban dwellers learn about and response to a natural hazard is of great significance as more and more people migrate to cities. Social media has become one of the most essential communication platforms in the virtual space for users to share their knowledge, information, and opinions about almost everything in the physical world. Geo-tagged posts published on different social media platforms contain a huge amount of information that can help us to better understand the dynamics of collective geo-tagged human activities. In this study, we investigated the spatiotemporal distribution patterns of the collective geo-tagged human activities in Beijing when it was afflicted by the “6-22” rainstorm. We used a variety of machine learning and statistical methods to examine the correlations between rainstorm-related microblogs and the rainstorm characteristics at a fine spatial and a fine temporal scale across Beijing. We also studied factors that could be used to explain the changes of the rainstorm-related blogging activities. Our results show that the human response to a disaster is very consistent, though with certain time lags, in the virtual and physical spaces at both the grid and city scales. Such a consistency varies significantly across our study area.
Effects of CNT Dosages in Cement Composites on the Mechanical Properties and Hydration Reaction with Low Water-to-Binder Ratio
Authors: Jung Sang-Hwa , Oh Sungwoo , Kim Sung-Wook , Moon Jae-Heum
Published: October 2019

This study aimed to investigate the potential use of carbon nanotubes (CNTs), which are an innovative construction material preferred by many researchers. Long-term microstructure enhancement and on-site application are major reasons to conduct research on CNT-cement composites; thus, a study on mechanical properties as well as the thermal conductivity of CNT-cement composites was carried out. As the CNT content increased, the thermal conductivity of CNT-cement composites was also enhanced. In addition, a couple of microstructure analyses such as isothermal calorimetry, thermal gravimetric analysis and SEM-EDS (Scanning Electron Microscope-Energy Dispersive X-ray Spectroscopy) for observing hydration reaction rate and types of hydration products were conducted to establish the advantage of CNT use in cement composites. Strength development of CNT-cement composites at early ages was slow, although eventually CNTs containing water developed equivalent level of strengths at last as internal curing effects.
Comparative Fatigue Life Assessment of Wind Turbine Blades Operating with Different Regulation Schemes
Authors: Loza Brian , Pacheco-Chérrez Josué , Cárdenas Diego , Minchala Luis I. , Probst Oliver
Published: October 2019

A comparative evaluation of the fatigue damage occurring in the blades of small wind turbines, with different power regulation schemes, has been conducted for the first time. Three representative test cases were built, one based on stall regulation and two using pitch regulation. The power curves were tuned to be identical in all cases, in order to allow for a direct comparison of fatigue damage. A methodology combining a dynamic simulation of a wind turbine forced by stochastic wind speed time series, with the application of the IEC 61400-2 standard, was designed and applied for two levels of turbulence intensity. The effect of the wind regime was studied by considering Weibull-distributed wind speeds with a variety of parameter sets. Not unexpectedly, in typical wind regimes, stall regulation led to a generally higher fatigue damage than pitch regulation, for similar structural blade design, but the practical implications were smaller than thought previously. Given the need for cost-effective designs for small wind turbines, stall regulation may be a viable alternative for off-grid applications.
A Feature Analysis Based Identifying Scheme Using GBDT for DDoS with Multiple Attack Vectors
Authors: Zhang Jian , Liang Qidi , Jiang Rui , Li Xi
Published: October 2019

In recent years, distributed denial of service (DDoS) attacks have increasingly shown the trend of multiattack vector composites, which has significantly improved the concealment and success rate of DDoS attacks. Therefore, improving the ubiquitous detection capability of DDoS attacks and accurately and quickly identifying DDoS attack traffic play an important role in later attack mitigation. This paper proposes a method to efficiently detect and identify multivector DDoS attacks. The detection algorithm is applicable to known and unknown DDoS attacks.
A Novel Sensing Strategy Based on Energy Detector for Spectrum Sensing
Authors: Huang Hai , Zhu Jia , Mu Junsheng
Published: October 2019

Sensing strategy directly influences the sensing accuracy of a spectrum sensing scheme. As a result, the optimization of a sensing strategy appears to be of great significance for accuracy improvement in spectrum sensing. Motivated by this, a novel sensing strategy is proposed in this paper, where an improved tradeoff among detection probability, false-alarm probability and available throughput is obtained based on the energy detector. We provide the optimal sensing performance and exhibit its superiority in theory compared with the classical scheme. Finally, simulations validate the conclusions drawn in this paper.
Circulating Regalia and Lakȟóta Survivance, c. 1900
Authors: Burns Emily C.
Published: October 2019

This essay offers object biographies of two examples of Lakȟóta beaded regalia that traveled with Wild West performers to France in 1889 and in 1911, respectively, as exemplars of Gerald Vizenor’s concept of survivance. By examining the production of the objects by women artists within the Lakȟóta community and visually analyzing their designs, this article highlights the regalia as an opposition to both settler colonial political suppression and enforced attempts of cultural assimilation. The article stresses that the beadwork’s materiality bears traces of its intended circulation and public display that are enacted when Lakȟóta individuals wore the regalia in the context of Wild West performance in France. Both when rooted in the Lakȟóta community and when circulating through Wild West shows, the objects evince Lakȟóta survivance. When the regalia was acquired by non-Native individuals in France, who projected new meanings onto the objects, the function of the regalia as a public statement of Lakȟóta survivance subtly continued to operate through generated revenue for the community and through the visibility of Lakȟóta culture through continued circulation.
Experience Design, Virtual Reality and Media Hybridization for the Digital Communication Inside Museums
Authors: Pietroni Eva
Published: October 2019

Experience design, both in real and in virtual museums, is very complex to be planned, even more when digital contents are juxtaposed to real collections. Researchers in this field, curators, creatives and software developers must work together in order to evolve towards a more efficient interconnection among visitors, collections and digital applications. This paper deals with such an interconnection, providing a theoretical background and practical guidelines, on the basis of museum studies and of the author’s research experience in this domain, supported by the results of surveys carried out on European museums visitors dealing with digital technologies. Media hybridization has a long and evolving tradition and it can contribute to transmit culture in engaging way to the public, respecting the scientific plausibility of contents. The choice of narrative structures and styles, as well as of interaction paradigms and technologies, is deeply conditioned by a series of factors that will be examined in detail. A general “direction” is needed to arouse in the visitor a feeling of confidence and trust, expectation and discovery that makes him/her feel at the center of an emotional and creative experience, of a progressive appropriation of meaning. Various typologies of experiences will be discussed and compared.
Investigating the Influence of the Implementation of an Energy Development Plan on Air Quality Using WRF-CAMx Modeling Tools: A Case Study of Shandong Province in China
Authors: Luo Ruoting , He Cheng , Yu Qi , He Li , Zhang Yan , Ma Weichun
Published: October 2019

In this paper, the Weather Research Forecast (WRF) Comprehensive Air Quality Model with Extensions (CAMx) modeling system with the particulate source apportionment technology (PSAT) module was used to study and analyze the spatial and temporal distribution of atmospheric pollutant concentrations and the source apportionment of fine particles (PM2.5) under the base year and an emission reduction scenario in the Shandong province, China. Our results show that industry is the largest contributor of PM2.5. In addition, the contribution of key energy-related industries was as high as 29.5%, with the thermal power industry being the largest individual contributor. In January, the largest contribution came from residents, reaching 41.3%. Moreover, loose coal burning in rural areas contributed up to 19.4% in winter. Our results also show that the emission reduction scenario had palpable effects on the reduction of air pollution. The more the emissions of SO2, NOX, PM2.5, and PM10 were reduced, the more the average concentration was decreased. The implementation of energy conservation and emission reduction policies by industry and resident is conducive to improving the quality of the atmospheric environment. In particular, a comprehensive control of loose coal burning in winter could significantly improve heavy pollution by particulate matter in winter.
Impact of Aerosol-Cloud Cycling on Aqueous Secondary Organic Aerosol Formation
Authors: Tsui William G. , Woo Joseph L. , McNeill V. Faye
Published: October 2019

Chemical processing of organic material in aqueous atmospheric aerosols and cloudwater is known to form secondary organic aerosols (SOA), although the extent to which each of these processes contributes to total aerosol mass is unclear. In this study, we use GAMMA 5.0, a photochemical box model with coupled gas and aqueous-phase chemistry, to consider the impact of aqueous organic reactions in both aqueous aerosols and clouds on isoprene epoxydiol (IEPOX) SOA over a range of pH for both aqueous phases, including cycling between cloud and aerosol within a single simulation. Low pH aqueous aerosol, in the absence of organic coatings or other morphology which may limit uptake of IEPOX, is found to be an efficient source of IEPOX SOA, consistent with previous work. Cloudwater at pH 4 or lower is also found to be a potentially significant source of IEPOX SOA. This phenomenon is primarily attributed to the relatively high uptake of IEPOX to clouds as a result of higher water content in clouds as compared with aerosol. For more acidic cloudwater, the aqueous organic material is comprised primarily of IEPOX SOA and lower-volatility organic acids. Both cloudwater pH and the time of day or sequence of aerosol-to-cloud or cloud-to-aerosol transitions impacted final aqueous SOA mass and composition in the simulations. The potential significance of cloud processing as a contributor to IEPOX SOA production could account for discrepancies between predicted IEPOX SOA mass from atmospheric models and measured ambient IEPOX SOA mass, or observations of IEPOX SOA in locations where mass transfer limitations are expected in aerosol particles.
Forecasting Particulate Matter Concentration Using Linear and Non-Linear Approaches for Air Quality Decision Support
Authors: Abdullah Samsuri , Ismail Marzuki , Ahmed Ali Najah , Abdullah Ahmad Makmom
Published: October 2019

Air quality status on the East Coast of Peninsular Malaysia is dominated by Particulate Matter (PM10) throughout the years. Studies have affirmed that PM10 influence human health and the environment. Therefore, precise forecasting algorithms are urgently needed to determine the PM10 status for mitigation plan and early warning purposes. This study investigates the forecasting performance of a linear (Multiple Linear Regression) and two non-linear models (Multi-Layer Perceptron and Radial Basis Function) utilizing meteorological and gaseous pollutants variables as input parameters from the year 2000–2014 at four sites with different surrounding activities of urban, sub-urban and rural areas. Non-linear model (Radial Basis Function) outperforms the linear model with the error reduced by 78.9% (urban), 32.1% (sub-urban) and 39.8% (rural). Association between PM10 and its contributing factors are complex and non-linear in nature, best captured by an Artificial Neural Network, which generates more accurate PM10 compared to the linear model. The results are robust enough for precise next day forecasting of PM10 concentration on the East Coast of Peninsular Malaysia.
On the Effect of Red Sea and Topography on Rainfall over Saudi Arabia: Case Study
Authors: Al-Mutairi Motirh , Abdel Basset Heshmat , Morsy Mostafa , Abdeldym Abdallah
Published: October 2019

This paper aimed to investigate the impact of Red Sea topography and water on the development and rainfall of a case of cyclogenesis occurs over Saudi Arabia during the period 16–18 November 2015 using the Weather Research and Forecasting (WRF) model. The WRF Control Run (WRF-CR) experiment was performed with presence of actual topography and surface water of the Red Sea, while the other three sensitivity experiments were carried out without (i) Red Sea Topography (NRST), (ii) Red Sea Water (NRSW), and (iii) Red Sea Topography and Water (NRSTW). The simulated rainfall in the control experiment depicts in well agreement with Tropical Rainfall Measurement Mission (TRMM) rainfall estimates in terms of intensity as well as spatio-temporal distribution. Results demonstrate that rainfall intensity and spatio-temporal distribution significantly changes through each sensitivity experiment compared to the WRF-CR, where the significant variation was found in the NRST experiment. The absence of topography (NRST) leads to formation of strong convergence area over the middle of Red Sea which enhanced uplift motion that further strengthened the low-level jet over Red Sea and the surrounding regions, which enhanced the moisture and temperature gradient and created a conditionally unstable atmosphere that favored the development of the cyclonic system. The absence of Red Sea water (NRSW) changed rainfall spatial distribution and reduced its amount by about 30–40% due to affecting of the dynamics of the upward motion and moisture gradient, suggesting that surface fluxes play an important role in regulating the low-level moist air convergence prior to convection initiation and development.
Autophagy in Cancer Cell Death
Authors: Linder Benedikt , Kögel Donat
Published: October 2019

Autophagy has important functions in maintaining energy metabolism under conditions of starvation and to alleviate stress by removal of damaged and potentially harmful cellular components. Therefore, autophagy represents a pro-survival stress response in the majority of cases. However, the role of autophagy in cell survival and cell death decisions is highly dependent on its extent, duration, and on the respective cellular context. An alternative pro-death function of autophagy has been consistently observed in different settings, in particular, in developmental cell death of lower organisms and in drug-induced cancer cell death. This cell death is referred to as autophagic cell death (ACD) or autophagy-dependent cell death (ADCD), a type of cellular demise that may act as a backup cell death program in apoptosis-deficient tumors. This pro-death function of autophagy may be exerted either via non-selective bulk autophagy or excessive (lethal) removal of mitochondria via selective mitophagy, opening new avenues for the therapeutic exploitation of autophagy/mitophagy in cancer treatment.
Non-Invasive Approach for Evaluation of Pulmonary Hypertension Using Extracellular Vesicle-Associated Small Non-Coding RNA
Authors: Lipps Christoph , Northe Philipp , Figueiredo Ricardo , Rohde Manfred , Brahmer Alexandra , Krämer-Albers Eva-Maria , Liebetrau Christoph , Wiedenroth Christoph B. , Mayer Eckhard , Kriechbaum Steffen D. , Dörr Oliver , Nef Holger , Hamm Christian W. , Keller Till , Troidl Christian
Published: October 2019

Extracellular vesicles are released by numerous cell types of the human body under physiological but also under pathophysiological conditions. They are important for cell–cell communication and carry specific signatures of peptides and RNAs. In this study, we aimed to determine whether extracellular vesicles isolated from patients with pulmonary hypertension show a disease specific signature of small non-coding RNAs and thus have the potential to serve as diagnostic and prognostic biomarkers. Extracellular vesicles were isolated from the serum of 23 patients with chronic thromboembolic pulmonary hypertension (CTEPH) and 23 controls using two individual methods: a column-based method or by precipitation. Extracellular vesicle- associated RNAs were analyzed by next-generation sequencing applying molecular barcoding, and differentially expressed small non-coding RNAs were validated by quantitative real-time polymerase chain reaction (qRT-PCR). We identified 18 microRNAs and 21 P-element induced wimpy testis (PIWI)-interacting RNAs (piRNAs) or piRNA clusters that were differentially expressed in CTEPH patients compared with controls. Bioinformatic analysis predicted a contribution of these piRNAs to the progression of cardiac and vascular remodeling. Expression levels of DQ593039 correlated with clinically meaningful parameters such as mean pulmonary arterial pressure, pulmonary vascular resistance, right ventricular systolic pressure, and levels of N-terminal pro-brain natriuretic peptide. Thus, we identified the extracellular vesicle- derived piRNA, DQ593039, as a potential biomarker for pulmonary hypertension and right heart disease.
Unravelling the Skin Secretion Peptides of the Gliding Leaf Frog, Agalychnis spurrelli (Hylidae)
Authors: Proaño-Bolaños Carolina , Blasco-Zúñiga Ailín , Almeida José Rafael , Wang Lei , Llumiquinga Miguel Angel , Rivera Miryan , Zhou Mei , Chen Tianbao , Shaw Chris
Published: October 2019

Frog skin secretions contain medically-valuable molecules, which are useful for the discovery of new biopharmaceuticals. The peptide profile of the skin secretion of Agalychnis spurrelli has not been investigated; therefore, the structural and biological characterization of its compounds signify an inestimable opportunity to acquire new biologically-active chemical scaffolds. In this work, skin secretion from this amphibian was analysed by molecular cloning and tandem mass spectrometry. Although the extent of this work was not exhaustive, eleven skin secretion peptides belonging to five peptide families were identified. Among these, we report the occurrence of two phyllokinins, and one medusin-SP which were previously reported in other related species. In addition, eight novel peptides were identified, including four dermaseptins, DRS-SP2 to DRS-SP5, one phylloseptin-SP1, and three orphan peptides. Phylloseptin-SP1 and dermaseptins-SP2 were identified in HPLC fractions based on their molecular masses determined by MALDI-TOF MS. Among the antimicrobial peptides, dermaseptin-SP2 was the most potent, inhibiting Escherichia coli, Staphylococcus aureus, and ORSA with a minimum inhibitory concentration (MIC) of 2.68 μM, and Candida albicans with an MIC of 10.71 μM, without haemolytic effects. The peptides described in this study represent but a superficial glance at the considerable structural diversity of bioactive peptides produced in the skin secretion of A. spurrelli.
FDA- and EMA-Approved Tyrosine Kinase Inhibitors in Advanced EGFR-Mutated Non-Small Cell Lung Cancer: Safety, Tolerability, Plasma Concentration Monitoring, and Management
Authors: Solassol Isabelle , Pinguet Frédéric , Quantin Xavier
Published: October 2019

Non-small-cell lung cancer (NSCLC) is the most common form of primary lung cancer. The discovery of several oncogenic driver mutations in patients with NSCLC has allowed the development of personalized treatments based on these specific molecular alterations, in particular in the tyrosine kinase (TK) domain of the epidermal growth factor receptor (EGFR) gene. Gefitinib, erlotinib, afatinib, and osimertinib are TK inhibitors (TKIs) that specifically target EGFR and are currently approved by the Food and Drug Administration (FDA) and the European Medicines Agency (EMA) as first line treatment for sensitive EGFR-mutant patients. However, these four drugs are associated with severe adverse events (AEs) that can significantly impact patient health-related quality of life and patient monitoring. EGFR-TKIs are commonly used together with other types of medication that can substantially interact. Here, we review approaches used for the management of TKI-AEs in patients with advanced NSCLC to promote the benefits of treatments and minimize the risk of TKI treatment discontinuation. We also consider potential TKI–drug interactions and discuss the usefulness of plasma concentration monitoring TKIs based on chromatographic and mass spectrometry approaches to guide clinical decision-making. Adjusting the most appropriate therapeutic strategies and drug doses may improve the performance therapy and prognosis of patients with advanced EGFR-mutated NSCLC.
Ethanol Impairs NRF2/Antioxidant and Growth Signaling in the Intact Placenta In Vivo and in Human Trophoblasts
Authors: Shanmugam Sambantham , Patel Dhyanesh , Wolpert John M. , Keshvani Caezaan , Liu Xiaobo , Bergeson Susan E. , Kidambi Srivatsan , Mahimainathan Lenin , Henderson George I. , Narasimhan Madhusudhanan
Published: October 2019

NRF2 is a redox-sensitive transcription factor that depending on the duration or magnitude of the stress, either translocates to the nucleus (beneficial) or is degraded in the cytosol (harmful). However, the role of NRF2-based mechanism(s) under ethanol (E)-induced developmental toxicity in the placental context remains unknown. Here, we used a rat prenatal model of maternal alcohol stress consisting of intermittent ethanol vapor (IEV) daily from GD11 to GD20 with a 6 h ON/18 h OFF in a vapor chamber and in vitro placental model consisting of HTR-8 trophoblasts exposed to 86 mM of E for either 24 h or 48 h. The role of NRF2 was evaluated through the NRF2-transactivation reporter assay, qRT-PCR, and Western blotting for NRF2 and cell growth-promoting protein, and cell proliferation assay. In utero and in vitro E decreased the nuclear NRF2 content and diminished its transactivation ability along with dysregulation of the proliferation indices, PCNA, CYCLIN-D1, and p21. This was associated with a ~50% reduction in cell proliferation in vitro in trophoblasts. Interestingly, this was found to be partially rescued by ectopic Nrf2 overexpression. These results indicate that ethanol-induced dysregulation of NRF2 coordinately regulates PCNA/CYCLIN-D1/p21 involving growth network, at least partially to set a stage for placental perturbations.
Elicitors and Pre-Fermentative Cold Maceration: Effects on Polyphenol Concentration in Monastrell Grapes and Wines
Authors: Paladines-Quezada Diego F. , Moreno-Olivares Juan D. , Fernández-Fernández José I. , Bleda-Sánchez Juan A. , Martínez-Moreno Alejandro , Gil-Muñoz Rocío
Published: October 2019

Vitis vinifera L cv Monastrell is the main red grape variety grown for vinification in the Denomination of Origin Jumilla (southeast Spain). Different strategies are still being tested to optimize available resources both in terms of the environment and to achieve wines with better organoleptic and functional characteristics. The objective of this work was to combine two strategies: the application of methyl jasmonate (MeJ), benzothiadiazole (BTH), and methyl jasmonate + benzothiadiazole (MeJ + BTH) elicitors to Monastrell leaves, and pre-fermentative cold maceration. During two seasons, the experiment was carried out to improve the extraction of the phenolic compounds, whose levels may have increased following the application of elicitors in the field, and to assess the effect of both strategies on the wine quality. Discriminant analysis revealed that independently of the meteorological conditions during both years, the pre-harvest response to the application of elicitors MeJ, BTH, and MeJ + BTH, induced increases in total anthocyanin concentration of the treated grapes, allowing the distinction of the treatments. This analysis also allowed the distinction between the types of maceration used, showing greater extraction of phenolic compounds by the application of low temperature, giving wines with a higher index of total phenols, a greater intensity of color, and a lower luminosity.
Characterization of the Ohmyungsamycin Biosynthetic Pathway and Generation of Derivatives with Improved Antituberculosis Activity
Authors: Kim Eunji , Shin Yern-Hyerk , Kim Tae Ho , Byun Woong Sub , Cui Jinsheng , Du Young Eun , Lim Hyung-Ju , Song Myoung Chong , Kwon An Sung , Kang Sang Hyeon , Shin Jongheon , Lee Sang Kook , Jang Jichan , Oh Dong-Chan , Yoon Yeo Joon
Published: October 2019

The cyclic depsipeptides ohmyungsamycin (OMS) A (1) and B (2), isolated from the marine-derived Streptomyces sp. SNJ042, contain two non-proteinogenic amino acid residues, β-hydroxy-l-phenylalanine (β-hydroxy-l-Phe) and 4-methoxy-l-tryptophan (4-methoxy-l-Trp). Draft genome sequencing of Streptomyces sp. SNJ042 revealed the OMS biosynthetic gene cluster consisting of a nonribosomal peptide synthetase (NRPS) gene and three genes for amino acid modification. By gene inactivation and analysis of the accumulated products, we found that OhmL, encoding a P450 gene, is an l-Phe β-hydroxylase. Furthermore, OhmK, encoding a Trp 2,3-dioxygenase homolog, and OhmJ, encoding an O-methyltransferase, are suggested to be involved in hydroxylation and O-methylation reactions, respectively, in the biosynthesis of 4-methoxy-l-Trp. In addition, the antiproliferative and antituberculosis activities of the OMS derivatives dehydroxy-OMS A (4) and demethoxy-OMS A (6) obtained from the mutant strains were evaluated in vitro. Interestingly, dehydroxy-OMS A (4) displayed significantly improved antituberculosis activity and decreased cytotoxicity compared to wild-type OMS A.
Impact of Plant Growth Promoting Rhizobacteria in the Orchestration of Lycopersicon esculentum Mill. Resistance to Plant Parasitic Nematodes: A Metabolomic Approach to Evaluate Defense Responses Under Field Conditions
Authors: Khanna Kanika , Sharma Anket , Ohri Puja , Bhardwaj Renu , Abd_Allah Elsayed F. , Hashem Abeer , Ahmad Parvaiz
Published: October 2019

The present study deals with biological control of Meloidogyne incognita in 45-days old Lycopersicon esculentum, inoculated with Pseudomonas aeruginosa(M1) and Burkholderia gladioli (M2). The improved plant growth and biomass of nematode infested Plant growth promoting rhizobacteria (PGPR) inoculated plants was observed. Remarkable reduction in the numbers of second stage juvenile (J2s), root galls was recorded after treatment of microbes relative to experimental controls. Moreover, the lowered activities of oxidative stress markers (H2O2 (hydrogen peroxide), O2− (superoxide anion), malondialdehyde (MDA)) was estimated in plants after rhizobacterial supplementation. Higher activities of enzymatic (SOD (Superoxide dismutase), POD (Guaiacol peroxidase), CAT (Catalase), GPOX (Glutathione peroxidase), APOX (Ascorbate peroxidase), GST (Glutathione-S-transferase), GR (Glutathione reductase), DHAR (Dehydroascorbate reductase), PPO (Polyphenol oxidase)) and non-enzymatic (glutathione, ascorbic acid, tocopherol) antioxidants were further determined in nematode infected plants following the addition of bacterial strains. The upregulation of photosynthetic activities were depicted by evaluating plant pigments and gas exchange attributes. An increase in the levels of phenolic compounds (total phenols, flavonoids, anthocyanins), osmoprotectants (total osmolytes, carbohydrates, reducing sugars, trehalose, proline, glycine betaine, free amino acids) and organic acids (fumaric, succinic, citric, malic acid) were reflected in infected plants, showing further enhancement after application of biocontrol agents. The study revealed the understanding of plant metabolism, along with the initiative to commercially exploit the biocontrol agents as an alternative to chemical nematicides in infected fields for sustainable agriculture.
Endothelial-Cell-Derived Human Secretory Leukocyte Protease Inhibitor (SLPI) Protects Cardiomyocytes against Ischemia/Reperfusion Injury
Authors: Kongpol Kantapich , Nernpermpisooth Nitirut , Prompunt Eakkapote , Kumphune Sarawut
Published: October 2019

Vascular endothelial cell (EC)-derived factors play an important role in endothelial–cardiomyocyte crosstalk and could save cardiomyocytes (CMs) from injury. The manipulation of endothelial cells to secrete protective factors could enhance cardioprotection. Secretory leukocyte protease inhibitor (SLPI) has been known to protect the heart. The goal of this study was to evaluate the in vitro paracrine protective effect and mechanisms of EC-derived human SLPI on cardiomyocytes subjected to hypoxia/reoxygenation (H/R) injury. Stable endothelial cells overexpressing human SLPI were generated from an endothelial cell line (EA.hy926). The cytoprotective effect was determined by cell survival assay. The results showed that endothelial-derived recombinant human SLPI (rhSLPI) reduced simulated ischemia/reperfusion (I/R)-(81.75% ± 1.42% vs. 60.27% ± 2.52%, p < 0.05) and hypoxia/reoxygenation (H/R)-induced EC injury (83.57% ± 1.78% vs. 63.07% ± 1.93%, p < 0.05). Moreover, co-culture of ECs overexpressing rhSLPI with CMs at ratios 1:1 and 1:3 or treatment with conditioned medium enhanced cell viability by 10.51–16.7% (co-culture) and 15.25–20.45% (conditioned medium) by reducing intracellular reactive oxygen species (ROS) production, the Bax/Bcl-2 expression ratio, caspase-3, and caspase-8, and in preconditioned CMs by activation of p38 MAPK and Akt survival kinase. In conclusion, this study showed for the first time that EC-derived rhSLPI provided cardio-vasculoprotective effects against I/R injury as a possible alternative therapeutic strategy for cardioprotection.
What Electrochemical Biosensors Can Do for Forensic Science? Unique Features and Applications
Authors: Yáñez-Sedeño Paloma , Agüí Lourdes , Campuzano Susana , Pingarrón José
Published: October 2019

This article critically discusses the latest advances in the use of voltammetric, amperometric, potentiometric, and impedimetric biosensors for forensic analysis. Highlighted examples that show the advantages of these tools to develop methods capable of detecting very small concentrations of analytes and provide selective determinations through analytical responses, without significant interferences from other components of the samples, are presented and discussed, thus stressing the great versatility and utility of electrochemical biosensors in this growing research field. To illustrate this, the determination of substances with forensic relevance by using electrochemical biosensors reported in the last five years (2015–2019) are reviewed. The different configurations of enzyme or affinity biosensors used to solve analytical problems related to forensic practice, with special attention to applications in complex samples, are considered. Main prospects, challenges to focus, such as the fabrication of devices for rapid analysis of target analytes directly on-site at the crime scene, or their widespread use and successful applications to complex samples of interest in forensic analysis, and future efforts, are also briefly discussed.
A Sustainable Biomineralization Approach for the Synthesis of Highly Fluorescent Ultra-Small Pt Nanoclusters
Authors: Balu Rajkamal , Knott Robert , Elvin Christopher M. , Hill Anita J. , R. Choudhury Namita , Dutta Naba K.
Published: October 2019

Herein we report the first example of a facile biomineralization process to produce ultra-small-sized highly fluorescent aqueous dispersions of platinum noble metal quantum clusters (Pt-NMQCs) using a multi-stimulus responsive, biomimetic intrinsically disordered protein (IDP), Rec1-resilin. We demonstrate that Rec1-resilin acts concurrently as the host, reducing agent, and stabilizer of the blue-green fluorescent Pt-NMQCs once they are being formed. The photophysical properties, quantum yield, and fluorescence lifetime measurements of the synthesized Pt-NMQCs were examined using UV-Vis and fluorescence spectroscopy. The oxidation state of the Pt-NMQCs was quantitatively analyzed using X-ray photoelectron spectroscopy. Both a small angle X-ray scattering technique and a modeling approach have been attempted to present a detailed understanding of the structure and conformational dynamics of Rec1-resilin as an IDP during the formation of the Pt-NMQCs. It has been demonstrated that the green fluorescent Pt-NMQCs exhibit a high quantum yield of ~7.0% and a lifetime of ~9.5 ns in aqueous media. The change in photoluminescence properties due to the inter-dot interactions between proximal dots and aggregation of the Pt-NMQCs by evaporation was also measured spectroscopically and discussed.
Myelin Pathology: Involvement of Molecular Chaperones and the Promise of Chaperonotherapy
Authors: Scalia Federica , Marino Gammazza Antonella , Conway de Macario Everly , Macario Alberto J. L. , Cappello Francesco
Published: October 2019

The process of axon myelination involves various proteins including molecular chaperones. Myelin alteration is a common feature in neurological diseases due to structural and functional abnormalities of one or more myelin proteins. Genetic proteinopathies may occur either in the presence of a normal chaperoning system, which is unable to assist the defective myelin protein in its folding and migration, or due to mutations in chaperone genes, leading to functional defects in assisting myelin maturation/migration. The latter are a subgroup of genetic chaperonopathies causing demyelination. In this brief review, we describe some paradigmatic examples pertaining to the chaperonins Hsp60 (HSPD1, or HSP60, or Cpn60) and CCT (chaperonin-containing TCP-1). Our aim is to make scientists and physicians aware of the possibility and advantages of classifying patients depending on the presence or absence of a chaperonopathy. In turn, this subclassification will allow the development of novel therapeutic strategies (chaperonotherapy) by using molecular chaperones as agents or targets for treatment.
Do EEG and Startle Reflex Modulation Vary with Self-Reported Aggression in Response to Violent Images?
Authors: Kunaharan Sajeev , Halpin Sean , Sitharthan Thiagarajan , Walla Peter
Published: October 2019

Increased violence and aggressive tendencies are a problem in much of the world and are often symptomatic of many other neurological and psychiatric conditions. Among clinicians, current methods of diagnosis of problem aggressive behaviour rely heavily on the use of self-report measures as described by the Diagnostic and Statistical Manual of Mental Disorders 5th Edition (DSM-5) and International Classification of Diseases 10th revision (ICD-10). This approach does not place adequate emphasis on objective measures that are potentially sensitive to processes not feeding into subjective self-report. Numerous studies provide evidence that attitudes and affective content can be processed without leading to verbalised output. This exploratory study aimed to determine whether individuals in the normal population, grouped by self-reported aggression, differed in subjective versus objective affective processing. Participants (N = 52) were grouped based on their responses to the Buss–Durkee Hostility Inventory. They were then presented with affect-inducing images while brain event-related potentials (ERPs) and startle reflex modulation (SRM) were recorded to determine non-language-based processes. Explicit valence and arousal ratings for each image were taken to determine subjective affective effects. Results indicated no significant group differences for explicit ratings and SRM. However, ERP results demonstrated significant group differences between the ‘pleasant’ and ‘violent’ emotion condition in the frontal, central and parietal areas across both hemispheres. These findings suggest that parts of the brain process affective stimuli different to what conscious appraisal comes up with in participants varying in self-reported aggression.
Earthquake Response of Cold-Formed Steel-Based Building Systems: An Overview of the Current State of the Art
Authors: Di Lorenzo Gianmaria , De Martino Attilio
Published: October 2019

Building systems fabricated with cold-formed steel (CFS) profiles and members made of wood, gypsum, or other materials allow solving a range of issues arising in common constructional elements thanks to their advantages, such as lightness, strength, durability, physical stability, sustainability, and cost-effectiveness. As a result of this inherent competitiveness of CFS based buildings, their use has been gradually increasing in recent years both in the field of structural systems as non-structural architectural components and, above all, in the area of earthquake resistant buildings, where lightness play a key role. After a general introduction, the paper gives an overview of the current codification and ongoing research on CFS non-structural architectural and structural systems. Finally, the main conclusions are summarised, and possible future developments are outlined.
From Newspaper Substrate to Nanotubes—Analysis of Carbonized Soot Grown on Kaolin Sized Newsprint
Authors: Brinson Bruce E. , Gangoli Varun Shenoy , Kumar Anjli , Hauge Robert H. , Adams W. Wade , Barron Andrew R.
Published: October 2019

Herein, we report the successful use of newspaper as a substrate for the growth of single-walled carbon nanotubes (SWCNTs) by chemical vapor deposition (CVD) with intriguing results demonstrating that (a) the large surface area of newspaper stock allows for SWCNT growth and (b) only newspaper produced with kaolin clay sizing allowed for SWCNT growth. Kaolin newsprint was impregnated with Al2O3 and Fe(NO3)3·9H2O (as precursors to FexOy nanoparticles), and calcined (30 min at 400 °C). The subsequent char residue was loaded into a CVD chamber and used as a substrate for SWCNT growth at 750 °C, using H2, C2H2, and water vapor as the growth gas. Samples of raw carbon soot exhibiting fluorescence spectra, indicative of SWCNTs, were further evaluated by resonant Raman spectroscopy, and by transmission electron microscopy (TEM). The calcinated substrate remnants were evaluated by scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDS). Experiments utilizing paper substrates produced with kaolin filler resulted in hybridized sp2–sp3 bonded carbon species. The soot was found to consist primarily of carbon nanotubes and bi-layer graphene in the form of collapsed nanotubes, also known as graphene nanoribbons (GNR).
Role of Focal Adhesion Kinase in Small-Cell Lung Cancer and Its Potential as a Therapeutic Target
Authors: Aboubakar Nana Frank , Vanderputten Marie , Ocak Sebahat
Published: October 2019

Small-cell lung cancer (SCLC) represents 15% of all lung cancers and it is clinically the most aggressive type, being characterized by a tendency for early metastasis, with two-thirds of the patients diagnosed with an extensive stage (ES) disease and a five-year overall survival (OS) as low as 5%. There are still no effective targeted therapies in SCLC despite improved understanding of the molecular steps leading to SCLC development and progression these last years. After four decades, the only modest improvement in OS of patients suffering from ES-SCLC has recently been shown in a trial combining atezolizumab, an anti-PD-L1 immune checkpoint inhibitor, with carboplatin and etoposide, chemotherapy agents. This highlights the need to pursue research efforts in this field. Focal adhesion kinase (FAK) is a non-receptor protein tyrosine kinase that is overexpressed and activated in several cancers, including SCLC, and contributing to cancer progression and metastasis through its important role in cell proliferation, survival, adhesion, spreading, migration, and invasion. FAK also plays a role in tumor immune evasion, epithelial-mesenchymal transition, DNA damage repair, radioresistance, and regulation of cancer stem cells. FAK is of particular interest in SCLC, being known for its aggressiveness. The inhibition of FAK in SCLC cell lines demonstrated significative decrease in cell proliferation, invasion, and migration, and induced cell cycle arrest and apoptosis. In this review, we will focus on the role of FAK in cancer cells and their microenvironment, and its potential as a therapeutic target in SCLC.
Automated Workflow for Somatic and Germline Next Generation Sequencing Analysis in Routine Clinical Cancer Diagnostics
Authors: Muscarella Lucia Anna , Fabrizio Federico Pio , De Bonis Maria , Mancini Maria Teresa , Balsamo Teresa , Graziano Paolo , Centra Flavia , Sparaneo Angelo , Trombetta Domenico , Bonfitto Antonio , Scagliusi Vito , Larizza Pietro , Capoluongo Ettore Domenico , Fazio Vito Michele
Published: October 2019

Thanks to personalized medicine trends and collaborations between industry, clinical research groups and regulatory agencies, next generation sequencing (NGS) is turning into a common practice faster than one could have originally expected. When considering clinical applications of NGS in oncology, a rapid workflow for DNA extraction from formalin-fixed paraffin-embedded (FFPE) tissue samples, as well as producing high quality library preparation, can be real challenges. Here we consider these targets and how applying effective automation technology to NGS workflows may help improve yield, timing and quality-control. We firstly evaluated DNA recovery from archived FFPE blocks from three different manual extraction methods and two automated extraction workstations. The workflow was then implemented to somatic (lung/colon panel) and germline (BRCA1/2) library preparation for NGS analysis exploiting two automated workstations. All commercial kits gave good results in terms of DNA yield and quality. On the other hand, the automated workstation workflow has been proven to be a valid automatic extraction system to obtain high quality DNA suitable for NGS analysis (lung/colon Ampli-seq panel). Moreover, it can be efficiently integrated with an open liquid handling platform to provide high-quality libraries from germline DNA with more reproducibility and high coverage for targeted sequences in less time (BRCA1/2). The introduction of automation in routine workflow leads to an improvement of NGS standardization and increased scale up of sample preparations, reducing labor and timing, with optimization of reagents and management.
Role of the Outer Metal of Double Metal Cyanides on the Catalytic Efficiency in Styrene Oxidation
Authors: Molina-Maldonado Paulina , Ruíz-Guerrero Rosario , Hernández-Fuentes Carlos
Published: October 2019

The catalytic efficiency of double metal cyanide (DMC) has been shown to be very effective in heterogeneous catalysis. The catalytic activity of the outer divalent cations (Mn, Co, Ni, and Cu) of a family of hexacyanocobaltates was examined in the oxidation reaction of styrene, as a model molecule, using tert Butyl Hydroperoxide (TBHP, Luperox®) as an oxidizing agent. The most electronegative outer cations showed the best conversions, with 95% for copper, followed by nickel with 85% conversion of the monomer at atmospheric pressure and temperature of 75 °C. The evidence showed that the catalytic activity and selectivity towards oxidized products are strongly linked to the accurate choice of the outer cation in the DMC together with the oxidizing agent.
Acceleration of Persulfate Activation by MIL-101(Fe) with Vacuum Thermal Activation: Effect of FeII/FeIII Mixed-Valence Center
Authors: Yang Jieyang , Zeng Zequan , Huang Zhanggen , Cui Yan
Published: October 2019

In this work, the activation effect of vacuum thermal treatment on MIL-101(Fe) (MIL: Materials of Institute Lavoisier) was investigated for the first time. It demonstrated that vacuum thermal activation could accelerate the activation of persulfate (PS) by MIL-101(Fe), and the enhancement of the catalytic capacity of MIL-101(Fe) was mainly attributed to the change in the FeII/FeIII mixed-valence center. The results of the SEM and XRD showed that vacuum thermal activation had a negligible effect on the crystal structure and particle morphology of MIL-101(Fe). Meanwhile, the higher temperature of vacuum thermal activation caused a higher relative content ratio of FeII/FeIII. A widely used azo dye, X-3B, was chosen as the probe molecule to investigate the catalytic performance of all samples. The results showed that the activated samples could remove X-3B more effectively, and the sample activated at 150 °C without regeneration could effectively activate PS to remove X-3B for at least 5 runs and approximately 900 min. This work highlights the often-overlooked activation effect of vacuum thermal treatment and provides a simple way to improve the catalytic capacity and reusability of MIL-101(Fe) which is beneficial for the application of MIL-101(Fe)/PS systems in azo dye wastewater treatment.