bluuebunny/crossref_metadata_2025 huggingface dataset:
A Review of Neutrino Decoupling from the Early Universe to the Current Universe
Authors: Akita Kensuke , Yamaguchi Masahide
Published: October 2022

We review the distortions of spectra of relic neutrinos due to the interactions with electrons, positrons, and neutrinos in the early universe. We solve integro-differential kinetic equations for the neutrino density matrix, including vacuum three-flavor neutrino oscillations, oscillations in electron and positron background, a collision term and finite temperature corrections to electron mass and electromagnetic plasma up to the next-to-leading order O(e3). After that, we estimate the effects of the spectral distortions in neutrino decoupling on the number density and energy density of the Cosmic Neutrino Background (CνB) in the current universe, and discuss the implications of these effects on the capture rates in direct detection of the CνB on tritium, with emphasis on the PTOLEMY-type experiment. In addition, we find a precise value of the effective number of neutrinos, Neff=3.044. However, QED corrections to weak interaction rates at order O(e2GF2) and forward scattering of neutrinos via their self-interactions have not been precisely taken into account in the whole literature so far. Recent studies suggest that these neglections might induce uncertainties of ±(10−3–10−4) in Neff.
WKB Approaches to Restore Time in Quantum Cosmology: Predictions and Shortcomings
Authors: Maniccia Giulia , De Angelis Mariaveronica , Montani Giovanni
Published: October 2022

In this review, we analyse different aspects concerning the possibility to separate a gravity-matter system into a part which lives close to a quasi-classical state and a “small” quantum subset. The considered approaches are all relying on a WKB expansion of the dynamics by an order parameter and the natural arena consists of the Bianchi universe minisuperspace. We first discuss how, limiting the WKB expansion to the first order of approximation, it is possible to recover for the quantum subsystem a Schrödinger equation, as written on the classical gravitational background. Then, after having tested the validity of the approximation scheme for the Bianchi I model, we give some applications for the quantum subsystem in the so-called “corner” configuration of the Bianchi IX model. We individualize the quantum variable in the small one of the two anisotropy degrees of freedom. The most surprising result is the possibility to obtain a non-singular Bianchi IX cosmology when the scenario is extrapolated backwards in time. In this respect, we provide some basic hints on the extension of this result to the generic cosmological solution. In the last part of the review, we consider the same scheme to the next order of approximation identifying the quantum subset as made of matter variables only. This way, we are considering the very fundamental problem of non-unitary morphology of the quantum gravity corrections to quantum field theory discussing some proposed reformulations. Instead of constructing the time dependence via that one of the classical gravitational variables on the label time as in previous works, we analyse a recent proposal to construct time by fixing a reference frame. This scheme can be reached both introducing the so-called “kinematical action”, as well as by the well-known Kuchar–Torre formulation. In both cases, the Schrödinger equation, amended for quantum gravity corrections, has the same morphology and we provide a cosmological implementation of the model, to elucidate its possible predictions.
Exploring the Fundamental Mechanism in Driving Highest-Velocity Ionized Outflows in Radio AGNs
Authors: Ayubinia Ashraf , Xue Yongquan , Woo Jong-Hak , Le Huynh Anh Nguyen , He Zhicheng , Miraghaei Halime , Lin Xiaozhi
Published: October 2022

We investigate the ionized gas kinematics relationship with X-ray, radio and accreting properties using a sample of 348 nearby (z<0.4) SDSS-FIRST-X-ray detected AGNs. X-ray properties of our sample are obtained from XMM-Newton, Swift and Chandra observations. We unveil the ionized gas outflows in our sample manifested by the non-gravitational broad component in [O iii]λ5007Å emission line profiles. From the comparison of the correlation of non-parametric outflow velocities (i.e., the velocity width, the maximal velocity of outflow and line dispersion) with X-ray luminosity and radio luminosity, we find that outflow velocities have similarly positive correlations with both X-ray and radio luminosity. After correcting for the gravitational component, we find that the [O iii] velocity dispersion normalized by stellar mass also increases with both X-ray luminosity and radio luminosity. We also find that, for a given X-ray (radio) luminosity, radio (X-ray) luminous AGNs have higher outflow velocities than non-radio (non-X-ray) luminous AGNs. Therefore, we find no clear preference between X-ray luminosity and radio luminosity in driving high-velocity ionized outflows and conclude that both AGN activity and small-scale jets contribute comparably. Moreover, there is no evidence that our obscured AGNs are preferentially associated with higher velocity outflows. Finally, we find a turning point around log(λEdd)≃−1.3 when we explore the dependency of outflow velocity on Eddington ratio. It can be interpreted considering the role of high radiation pressure (log(λEdd)≳−1.3) in causing drastic reduction in the covering factor of the circumnuclear materials.
Association of ScV-LA Virus with Host Protein Metabolism Determined by Proteomics Analysis and Cognate RNA Sequencing
Authors: Lukša Juliana , Celitan Enrika , Servienė Elena , Serva Saulius
Published: October 2022

Saccharomyces yeasts are highly dispersed in the environment and microbiota of higher organisms. The yeast killing phenotype, encoded by the viral system, was discovered to be a significant property for host survival. Minor alterations in transcription patterns underpin the reciprocal relationship between LA and M viruses and their hosts, suggesting the fine-tuning of the transcriptional landscape. To uncover the principal targets of both viruses, we performed proteomics analysis of virus-enriched subsets of host proteins in virus type-specific manner. The essential pathways of protein metabolism–from biosynthesis and folding to degradation–were found substantially enriched in virus-linked subsets. The fractionation of viruses allowed separation of virus-linked host RNAs, investigated by high-content RNA sequencing. Ribosomal RNA was found to be inherently associated with LA-lus virus, along with other RNAs essential for ribosome biogenesis. This study provides a unique portrayal of yeast virions through the characterization of the associated proteome and cognate RNAs, and offers a background for understanding ScV-LA viral infection persistency.
A Review of Potential Therapeutic Strategies for COVID-19
Authors: Meng Jiajia , Li Ruijiao , Zhang Zhiqi , Wang Jie , Huang Qingwen , Nie Dongxia , Fan Kai , Guo Wenbo , Zhao Zhihui , Han Zheng
Published: October 2022

Coronavirus disease 2019 is a rather heterogeneous disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The ongoing pandemic is a global threat with increasing death tolls worldwide. SARS-CoV-2 belongs to lineage B β-CoV, a subgroup of Sarbecovirus. These enveloped, large, positive-sense single-stranded RNA viruses are easily spread among individuals, mainly via the respiratory system and droplets. Although the disease has been gradually controlled in many countries, once social restrictions are relaxed the virus may rebound, leading to a more severe and uncontrollable situation again, as occurred in Shanghai, China, in 2022. The current global health threat calls for the urgent development of effective therapeutic options for the treatment and prevention of SARS-CoV-2 infection. This systematic overview of possible SARS-CoV-2 therapeutic strategies from 2019 to 2022 indicates three potential targets: virus entry, virus replication, and the immune system. The information provided in this review will aid the development of more potent and specific antiviral compounds.
Sustained Virologic Suppression Reduces HIV-1 DNA Proviral Levels and HIV Antibodies in Perinatally HIV-Infected Children Followed from Birth
Authors: Fuller Trevon , Kerin Tara , Cortado Ruth , Benamor Teixeira Maria de Lourdes , Fragoso da Silveira Gouvêa Maria Isabel Fragoso da Silveira , Moreira Christianne , Santos Cruz Maria Leticia , Pilotto José Henrique , Gomes Ivete , Santos Breno , Rocha Tauí , Soni Priya R. , Joao Esau , Shin-Sim Myung , Bryson Yvonne , Nielsen-Saines Karin
Published: October 2022

The extent to which perinatally HIV-infected children, following cART initiation, develop a low proviral reservoir burden over time, as measured by HIV DNA droplet-digital polymerase chain reaction (ddPCR) and the effect on HIV antibody is not well characterized. We measured proviral HIV DNA and plasma RNA virus load (VL) in 37 perinatally HIV-infected children at 6 months of age who initiated stable cART. At 6–11 years of age, HIV proviral DNA, HIV VL (RNA), and HIV antibody by Western Blot (WB) were assessed. CART was initiated before 6 months of age in 13 children and after 6 months in 24. At school age, the HIV DNA levels did not differ by the timing of cART, and the HIV DNA levels were lower in children with negative/indeterminate WB (p = 0.0256). Children with undetectable HIV RNA VL > 50% of the time since cART initiation had lower median DNA VL than children with undetectable VL < 50% of the time (p = 0.07). Long-term viral suppression in perinatally HIV-infected children is associated with a decrease in HIV antibodies and reduced HIV reservoirs.
The Elevated De Ritis Ratio on Admission Is Independently Associated with Mortality in COVID-19 Patients
Authors: Drácz Bálint , Czompa Diána , Müllner Katalin , Hagymási Krisztina , Miheller Pál , Székely Hajnal , Papp Veronika , Horváth Miklós , Hritz István , Szijártó Attila , Werling Klára
Published: October 2022

Liver damage in COVID-19 patients was documented as increased alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels or an elevated AST/ALT ratio, known as the De Ritis ratio. However, the prognostic value of the elevated De Ritis ratio in COVID-19 patients is still unknown. The aim of our study was to evaluate the prognostic value of the De Ritis ratio compared to other abnormal laboratory parameters and its relation to mortality. We selected 322 COVID-19 patients in this retrospective study conducted between November 2020 and March 2021. The laboratory parameters were measured on admission and followed till patient discharge or death. Of the 322 COVID-19 patients, 57 (17.7%) had gastrointestinal symptoms on admission. The multivariate analysis showed that the De Ritis ratio was an independent risk factor for mortality, with an OR of 29.967 (95% CI 5.266–170.514). In ROC analysis, the AUC value of the the De Ritis ratio was 0.85 (95% CI 0.777–0.923, p < 0.05) with sensitivity and specificity of 80.6% and 75.2%, respectively. A De Ritis ratio ≥1.218 was significantly associated with patient mortality, disease severity, higher AST and IL-6 levels, and a lower ALT level. An elevated De Ritis ratio on admission is independently associated with mortality in COVID-19 patients, indicating liver injury and cytokine release syndrome.
Tick-Borne Encephalitis Virus Prevalence in Sheep, Wild Boar and Ticks in Belgium
Authors: Adjadj Nadjah Radia , Vervaeke Muriel , Sohier Charlotte , Cargnel Mickaël , De Regge Nick
Published: October 2022

Tick-borne encephalitis virus (TBEV) is the most important tick-borne zoonotic virus in Europe. In Belgium, antibodies to TBEV have already been detected in wildlife and domestic animals, but up-to-date prevalence data for TBEV are lacking, and no studies have assessed its seroprevalence in sheep. Serum samples of 480 sheep from all over Belgium and 831 wild boar hunted in Flanders (northern Belgium) were therefore screened for TBEV antibodies by ELISA and plaque reduction neutralization test (PRNT), respectively. The specificity of positive samples was assessed by PRNTs for TBEV and the Louping Ill, West Nile, and Usutu viruses. TBEV seroprevalence was 0.42% (2/480, CI 95%: 0.11–1.51) in sheep and 9.27% (77/831, CI 95%: 7.48–11.43) in wild boar. TBEV seroprevalence in wild boar from the province of Flemish Brabant was significantly higher (22.38%, 15/67) compared to Limburg (7.74%, 34/439) and Antwerp (8.61%, 28/325). Oud-Heverlee was the hunting area harboring the highest TBEV seroprevalence (33.33%, 11/33). In an attempt to obtain a Belgian TBEV isolate, 1983 ticks collected in areas showing the highest TBEV seroprevalence in wild boars were tested by real-time qPCR. No TBEV-RNA-positive tick was detected. The results of this study suggest an increase in TBEV prevalence over the last decade and highlight the need for One-Health surveillance in Belgium.
SARS-CoV-2: Searching for the Missing Variants
Authors: Caputo Emilia , Mandrich Luigi
Published: October 2022

Structural and phylogenetic analysis of the spike glycoprotein highlighted that the last variants, annotated as omicron, have about 30 mutations compared to the initial version reported in China, while the delta variant, supposed to be the omicron ancestor, shows only 7 mutations. Moreover, the five omicron variants were isolated between November 2021 and January 2022, and the last variant BA.2.75, unofficially named centaurus, was isolated in May 2022. It appears that, since the isolation of the delta variant (October 2020) to the omicron BA.1 (November 2021), there was an interval of one year, whereas the five omicron variants were isolated in three months, and after a successive four months period, the BA.2.75 variant was isolated. So, what is the temporal and phylogenetic correlation among all these variants? The analysis of common mutations among delta and the omicron variants revealed: (i) a phylogenetic correlation among these variants; (ii) the existence of BA.1 and BA.2 omicron variants a few months before being isolated; (iii) at least three possible intermediate variants during the evolution of omicron; (iv) the evolution of the BA.2.12.1, BA.4 and BA.5 variants from omicron BA.2; (v) the centaurus variant evolution from omicron BA.2.12.1.
New Insights into the Estimation of Reproduction Numbers during an Epidemic
Authors: Sebastiani Giovanni , Spassiani Ilaria
Published: October 2022

In this paper, we deal with the problem of estimating the reproduction number Rt during an epidemic, as it represents one of the most used indicators to study and control this phenomenon. In particular, we focus on two issues. First, to estimate Rt, we consider the use of positive test case data as an alternative to the first symptoms data, which are typically used. We both theoretically and empirically study the relationship between the two approaches. Second, we modify a method for estimating Rt during an epidemic that is widely used by public institutions in several countries worldwide. Our procedure is not affected by the problems deriving from the hypothesis of Rt local constancy, which is assumed in the standard approach. We illustrate the results obtained by applying the proposed methodologies to real and simulated SARS-CoV-2 datasets. In both cases, we also apply some specific methods to reduce systematic and random errors affecting the data. Our results show that the Rt during an epidemic can be estimated by using the positive test data, and that our estimator outperforms the standard estimator that makes use of the first symptoms data. It is hoped that the techniques proposed here could help in the study and control of epidemics, particularly the current SARS-CoV-2 pandemic.
The Correlates & Public Health Consequences of Prospective Vaccine Hesitancy among Individuals Who Received COVID-19 Vaccine Boosters in the U.S.
Authors: Motta Matt
Published: October 2022

Supplemental “booster” vaccines may prove vital in combating variant waves of endemic COVID-19. Given relatively low levels of booster vaccine uptake, Americans’ willingness to receive a second booster shot is unclear. In a demographically representative survey of N = 3950 US adults (limited to a “boosted” subsample of N = 1551 who had not yet received a second booster), 49% [95% CI: 47, 51] of Americans report having received an initial booster shot, while just 34% [33, 36] report that they would be “very likely” to do so again. Concerns about missing work to vaccinate (−10%; B = 0.53, p = 0.05) and being unconvinced that additional boosters will be necessary (−47%; B = 2.24, p < 0.01) are significantly and negatively associated with being very likely to receive a second COVID-19 booster. These findings can help inform discussions about policies aimed at increasing vaccine uptake in the U.S., and broaden researchers’ understanding of vaccine reluctance among those who might otherwise hold positive views toward vaccination.
COVID-19 Vaccine-Related Vogt–Koyanagi–Harada Disease Complicated by Central Serous Chorioretinopathy during Treatment Course: Case Report and Literature Review
Authors: Han Ruyi , Xu Gezhi , Ding Xinyi
Published: October 2022

With the promotion of mass COVID-19 vaccination in the elimination of the SARS-CoV-2 pandemic, new side effects, including ocular complications, are emerging. In this study, we report on a 62-year-old Chinese man who developed Vogt–Koyanagi–Harada (VKH) disease six days after his third dose of an inactivated COVID-19 vaccine, with a preceding severe headache and tinnitus. His medical history included tuberculosis 20 years prior and hypertension. Systemic prednisone was administered, resulting in completely relieved inflammation and improved visual acuity. Another three and a half months later, the visual acuity of his right eye slightly decreased due to complicated central serous chorioretinopathy (CSC) disease. By gradually replacing prednisone with cyclosporine within 2 months, the subretinal fluid was completely absorbed at the last visit. Steroid-related CSC during the treatment course of VKH disease after COVID-19 vaccination has never been reported before. By reviewing relative literature, we discuss the mechanism of CSC onset in our case and the potential therapeutic strategies. Complicated CSC may develop in the eyes with vaccine-related VKH after steroid treatment. Ophthalmologists should be aware of this condition, carefully distinguish complicated CSC with inflammation relapse, and adjust the medication in a timely manner.
Factors Influencing the Choice to Advise for or against COVID-19 Vaccination in the Physicians and Dentists of an Italian Region
Authors: Marziali Eleonora , Lontano Alberto , Regazzi Luca , Paladini Andrea , Villani Leonardo , Calabrò Giovanna Elisa , Damiani Gianfranco , Laurenti Patrizia , Ricciardi Walter , Cadeddu Chiara
Published: October 2022

Healthcare workers (HCWs), particularly physicians, are a relevant and trusted source of information for patients, especially when health-related choices such as vaccination are concerned. Between July and November 2022, we administered a web-based survey to physicians and dentists living in the Latio region of Italy to explore whether their background might affect their willingness to recommend the COVID-19 vaccination to their patients (RCVtoPat) and their relatives (RCVtoRel). We performed a multivariable logistic regression to study the association between the two outcomes (RCVtoPat and RCVtoRel) and their potential determinants in our sample (n = 1464). We found that being a dentist, an increasing fear of COVID-19, and having been previously vaccinated against flu are positively associated with both RCVtoPat and RCVtoRel, while a better self-rated knowledge of COVID-19 vaccines is associated only with RCVtoRel. No role was found for age, sex, civil status, education level, information sources, previous SARS-CoV-2 infection, and chronic diseases. A sub-group analysis of physicians alone (n = 1305) demonstrated a positive association with RCVtoRel of being specialized in diagnostic/therapeutic services and a negative effect on RCVtoPat of being trained in general practice. We provide useful insights about the factors that should be addressed to ensure HCWs exert a positive influence on their patients and communities.
An Overview of Rift Valley Fever Vaccine Development Strategies
Authors: Kitandwe Paul Kato , McKay Paul F. , Kaleebu Pontiano , Shattock Robin J.
Published: October 2022

Rift Valley fever (RVF) is a mosquito-borne viral zoonosis that causes high fetal and neonatal mortality in ruminants and a mild to fatal hemorrhagic fever in humans. There are no licensed RVF vaccines for human use while for livestock, commercially available vaccines are all either live attenuated or inactivated and have undesirable characteristics. The live attenuated RVF vaccines are associated with teratogenicity and residual virulence in ruminants while the inactivated ones require multiple immunisations to induce and maintain protective immunity. Additionally, nearly all licensed RVF vaccines lack the differentiating infected from vaccinated animals (DIVA) property making them inappropriate for use in RVF nonendemic countries. To address these limitations, novel DIVA-compatible RVF vaccines with better safety and efficacy than the licensed ones are being developed, aided fundamentally by a better understanding of the molecular biology of the RVF virus and advancements in recombinant DNA technology. For some of these candidate RVF vaccines, sterilizing immunity has been demonstrated in the discovery/feasibility phase with minimal adverse effects. This review highlights the progress made to date in RVF vaccine research and development and discusses the outstanding research gaps.
Access to COVID-19 Vaccines: A New Global Approach
Authors: Bouderhem Rabaï
Published: October 2022

This paper addresses the legal aspects and unprecedented consequences of the Coronavirus disease 2019 (COVID-19) pandemic on the manufacturing and fair access to vaccines. A research literature review allowed us to identify and evaluate the weaknesses of international health law to combat global health crises such as the COVID-19 pandemic. A new paradigm must encourage World Health Organization (WHO) and World Trade Organization (WTO) members to explicitly consider vaccines as global public goods and adopt a new set of legally binding rules for a fair and unrestricted access in times of pandemic. Initiatives and mechanisms such as COVID-19 Vaccine Global Access Facility (COVAX) have been developed to tackle the pandemic and allow developing countries to access vaccines but most were limited and never reached the expected results. The key role played by the WHO in global health policy needs to be strengthened throughout the revision of the International Health Regulations (IHR). Globalization and health are interconnected: WTO members shall revise the Agreement on Trade-Related Aspects of Intellectual Property Rights (TRIPS Agreement) and grant permanent intellectual property (IP) waivers on vaccines in times of pandemic. Our postulation is that vaccines constitute global public goods; their manufacturing and access must be facilitated and guaranteed by specific rules and mechanisms under the supervision of both the WHO and the WTO. It is, therefore, essential to provide the WHO with new powers and attributions to impose coordinated health policies to combat diseases and global crises such as the COVID-19 pandemic.
Evaluation of the Efficacy of COVID-19 Booster Vaccinations in Healthcare Personnel
Authors: Kim Chung-Jong , Bae Ji-Yun , Jun Kang-Il , Kim Jihee , Son Hee-Jung , Chung Hae-Sun , Kim Soo-Kyung , Kim Soohyun , Minn Dohsik , Choi Hee-Jung
Published: October 2022

This study aimed to investigate the efficacy of different COVID-19 booster vaccines by measuring the serum antibody titer. SARS-CoV-2 anti-nucleocapsid protein antibody (N-Ab), anti-spike protein antibody (S-Ab), and neutralizing antibody (Neut.Ab) were measured before and 4–6 weeks after booster vaccinations in healthcare personnel with a previous vaccination within 3–6 months. Personnel who previously received two doses of ChAdOx1 vaccine or two doses of BNT162b2 vaccine received the BNT162b2 vaccine (AAP and PPP groups, respectively). Personnel who previously received two doses of mRNA-1273 received the same vaccine as a booster dose (MMM group). Of the 917 participants, the AAP, MMM, and PPP groups comprised 837 (91.3%), 27 (2.9%), and 53 (5.8%) participants, respectively. The pre-booster S-Ab and Neut.Ab titer were significantly lower in the AAP group. After the booster vaccination, all participants were positive for S-Ab and Neut.Ab; furthermore, the S-Ab and Neut.Ab titer significantly increased in all three groups, although the post-booster S-Ab was lower in the AAP group than in the other groups. The post-booster Neut.Ab titer showed no significant difference among the groups. Our study’s results suggest that booster vaccination, after two prior vaccinations, shows a significant effect regardless of the type of vaccine administered.
Can a Two-Dose Influenza Vaccine Regimen Better Protect Older Adults? An Agent-Based Modeling Study
Authors: Williams Katherine V. , Krauland Mary G. , Harrison Lee H. , Williams John V. , Roberts Mark S. , Zimmerman Richard K.
Published: October 2022

Older adults (age ≥ 65) are at high risk of influenza morbidity and mortality. This study evaluated the impact of a hypothetical two-dose influenza vaccine regimen per season to reduce symptomatic flu cases by providing preseason (first dose) and mid-season (second dose) protection to offset waning vaccine effectiveness (VE). The Framework for Reconstructing Epidemiological Dynamics (FRED), an agent-based modeling platform, was used to compare typical one-dose vaccination to a two-dose vaccination strategy. Primary models incorporated waning VE of 10% per month and varied influenza season timing (December through March) to estimate cases and hospitalizations in older adults. Additional scenarios modeled reductions in uptake and VE of the second dose, and overall waning. In seasons with later peaks, two vaccine doses had the largest potential to reduce cases (14.4% with February peak, 18.7% with March peak) and hospitalizations (13.1% with February peak, 16.8% with March peak). Reductions in cases and hospitalizations still resulted but decreased when 30% of individuals failed to receive a second dose, second dose VE was reduced, or overall waning was reduced to 7% per month. Agent-based modeling indicates that two influenza vaccine doses could decrease cases and hospitalizations in older individuals. The highest impact occurred in the more frequently observed late-peak seasons. The beneficial impact of the two-dose regimen persisted despite model scenarios of reduced uptake of the second dose, decreased VE of the second dose, or overall VE waning.
Comparison of the Safety and Immunogenicity of FAKHRAVAC and BBIBP-CorV Vaccines when Administrated as Booster Dose: A Parallel Two Arms, Randomized, Double Blind Clinical Trial
Authors: Ahi Mohammadreza , Hamidi Farahani Ramin , Basiri Pouria , Karimi Rahjerdi Ahmad , Sheidaei Ali , Gohari Kimiya , Rahimi Zahra , Gholami Fatemeh , Moradi Milad , Ghafoori Naeeni Farzad , Saffar Kosar Naderi , Ghasemi Soheil , Barati Babak , Moradi Sohrab , Monazah Arina , Pouranvari Fatemeh , Forooghizadeh Mohsen
Published: October 2022

Purpose: This study was completed to assess the immunogenicity and safety of the FAKHRAVAC and BBIBP-CorV vaccines as a booster dose in the population with a history of receiving two doses of BBIBP-CorV vaccine. Methods: In this double-blind, parallel clinical trial, we randomly assigned healthy adults with a history of receiving two doses of the BBIBP-CorV vaccine, who then received either the FAKHRAVAC or BBIBP-CorV vaccine as a booster dose. The trial is registered in the Iranian Registry of Clinical Trial document depository (Code: IRCT20210206050259N4). Results: The outcomes that were monitored in this study were serum neutralizing antibody (Nab) activity, immunoglobulin G (IgG) level, local and systemic adverse reactions, serious adverse events, suspected unexpected serious adverse reactions, and medically attended adverse events. After administering vaccines to 435 participants, the most frequent local and systemic adverse reactions were tenderness and nausea in 23.7% and 1.4% of cases, respectively. All adverse events were mild, occurred at a similar incidence in the two groups, and were resolved within a few days. Conclusions: On the 14th day after the booster dose injection, the seroconversion rate (i.e., four-fold increase) of Nabs for seronegative participants were 87% and 84.6% in the FAKHRAVAC® and BBIBP-CorV groups, respectively. This study shows that the FAKHRAVAC® vaccine, as a booster dose, has a similar function to the BBIBP-CorV vaccine in terms of increasing the titer of virus-neutralizing antibodies, the amount of specific antibodies, and safety.
The Anti-Tumor and Immunomodulatory Effects of PLGA-Based Docetaxel Nanoparticles in Lung Cancer: The Potential Involvement of Necroptotic Cell Death through Reactive Oxygen Species and Calcium Build-Up
Authors: Gupta Parul , Singh Arpita , Verma Ajay , Kant Surya , Pandey Anuj , Khare Puneet , Prakash Ved
Published: October 2022

Taxanes, microtubule stabilizing agents, are extensively used in the treatment of non-small cell lung cancer (NSCLC). However, their clinical effectiveness remains restricted owing to significant adverse effects and drug resistance. Nanotechnology may guide chemotherapeutic drugs directly and selectively to malignant cells, improving their therapeutic efficiency. In the present study, we synthesized polylactic-co-glycolic acid (PLGA) based nanoparticles encapsulating docetaxel and evaluated their efficacy in non-small cell lung carcinoma (A549) cells and primary immune cells derived from humans. Docetaxel–PLGA nanoparticles (PLGA-Dtx) were synthesized and characterized using distinct methods. Moreover, the cytotoxicity of free docetaxel (Dtx) and Dtx-conjugated nanoparticles (PLGA-Dtx) was studied in A549 cells and peripheral blood mononuclear cells derived from humans. Furthermore, annexin V-FITC/PI staining was used to assess the mode of cell death. Additionally, human peripheral blood mononuclear cells (PBMCs) were used for assessing the associated immune response and cytokine profile following PLGA-Dtx treatment. Spherical PLGA-Dtx nanoparticles with a 150 ± 10 nm diameter and 70% encapsulation efficiency (EE) were synthesized. The MTT assay showed that the IC50 of PLGA-Dtx nanoparticles was significantly lower than free docetaxel in A549 cells. Cytotoxicity data also revealed the selective nature of PLGA-Dtx with no significant effects in normal human bronchial epithelial cells (BEAS-2B) and PBMCs derived from healthy donors. Interestingly, PLGA-Dtx exerted an improved effect and tempted both apoptosis and necroptosis, as evidenced by annexin V and propidium iodide–positive cells. Further, PLGA-Dtx-exposed A549 cells showed increased Cas-3, Cas-9, RIP-1, and RIP-3, indicating apoptosis and necroptosis. An increased pro-inflammatory response manifested from the enhancement of IFN-γ and TNF-α in PLGA-Dtx-exposed PBMCs, posed by the occurrence of necroptosis and the immune stimulatory effect of PLGA-Dtx. In conclusion, PLGA-Dtx has a selective anticancer potential and better immunostimulatory potential. Therefore, PLGA-Dtx may be useful for the treatment of non-small cell lung carcinoma.
Predictors of Influenza Vaccination among Chinese Middle School Students Based on the Health Belief Model: A Mixed-Methods Study
Authors: Asihaer Yeerlin , Sun Mengyang , Li Miao , Xiao Huidi , Amaerjiang Nubiya , Guan Mengying , Thapa Bipin , Hu Yifei
Published: October 2022

Influenza vaccination rates among Chinese middle school students are low. This study aims to explore the influencing factors of vaccination among middle school students and promote vaccination. We conducted a mixed-methods study, integrating a questionnaire survey among 9145 middle school students in four cities in China and semi-structured interviews with 35 middle school students to understand their attitudes and perceptions toward vaccination based on the Health Belief Model. We found the overall vaccination rate was 38.2% (3493/9145), with students in Beijing, boarding at school, or senior high school showing higher values than their counterparts (p < 0.05). Multiple logistic regression results showed that non-boarding (OR = 0.46, 95%CI: 0.42–0.51) and perceived barriers (OR = 0.97, 95%CI: 0.96–0.98) were unfavorable factors for influenza vaccination, whereas perceived susceptibility (OR = 1.07, 95%CI: 1.05–1.08), perceived benefits (OR = 1.02, 95%CI: 1.01–1.04), cues to action (OR = 1.08, 95%CI: 1.05–1.11), and self-efficacy (OR = 1.04, 95%CI: 1.02–1.07) were facilitators. Qualitative results indicated that positive health beliefs, school, and the home environment contribute to vaccination. In conclusion, the influenza vaccination rate among middle school students remains low. The concerns about the safety and potential side effects of vaccines are the main barriers to vaccination, underscoring the need for strengthening communication, education, and information among students and their teachers/parents.
Sputnik Light and Sputnik V Vaccination Is Effective at Protecting Medical Personnel from COVID-19 during the Period of Delta Variant Dominance
Authors: Sukhikh Gennady T. , Priputnevich Tatiana V. , Ogarkova Darya A. , Pochtovyi Andrei A. , Kustova Daria D. , Zlobin Vladimir I. , Logunov Denis Y. , Gushchin Vladimir A. , Gintsburg Alexander L.
Published: October 2022

Medical personnel are a group of people that often encounter infectious agents, leading to greater risk of contracting infectious diseases. Specific prevention of diseases in this group is a priority. The epidemiological effectiveness of COVID-19 prevention in the group of medical workers due to the emergence of new variants of concern of the SARS-CoV-2 virus has not been studied in sufficient depth. We conducted a study of the effectiveness of vaccine use to protect medical workers at a large medical center for obstetrics and gynecology in Moscow. Sputnik V and Sputnik Light were the main vaccines used for the prevention of COVID-19. The vaccines are based on a variant of the S-protein of the SARS-CoV-2 virus, with adenovirus serotypes 5 and 26 as the vector for delivery. Vaccination of employees occurred during the period in which the Delta variant was spreading. The overall epidemiological effectiveness was 81.7% (73.1–87.6%) during the period in which the Delta variant was dominant. During the period from the beginning of vaccination (26 November 2020) until 8 February 2022, the overall effectiveness was 89.1% (86.9–91.0%). As expected, the highest effectiveness during this period was obtained in the group that received the third and fourth doses—96.5% (75.0–99.5%). The severity of COVID-19 in the vaccinated group was significantly lower than in the unvaccinated group.
Development of Bivalent mRNA Vaccines against SARS-CoV-2 Variants
Authors: Li Jianglong , Liu Qi , Liu Jun , Fang Zihui , Luo Liping , Li Shuang , Lei Yixin , Li Zhi , Jin Jing , Xie Ronglin , Peng Yucai
Published: October 2022

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has infected billions of individuals and is the cause of the current global coronavirus disease 2019 (COVID-19) pandemic. We previously developed an mRNA vaccine (LVRNA009) based on the S protein of the Wuhan-Hu-1 strain; the phases I and II clinical trials showed that LVRNA009 has a promising safety and immunogenicity profile. In order to counteract the immune escape by SARS-CoV-2 variants of concern, a panel of mRNA vaccines was developed based on the S proteins of the Wuhan-Hu-1, Delta, Omicron BA.1, BA.2, and BA.5 strains, and each vaccine’s protective potency against the virus variants was evaluated. Furthermore, to achieve excellent neutralization against SARS-CoV-2 variants, bivalent vaccines were developed and tested against the variants. We found that the monovalent Wuhan-Hu-1 or the Delta vaccines could induce high level of neutralization antibody and protect animals from the infection of the SARS-CoV-2 Wuhan-Hu-1 or Delta strains, respectively. However, serum samples from mice immunized with monovalent Delta vaccine showed relatively low virus neutralization titers (VNTs) against the pseudotyped virus of the Omicron strains. Serum samples from mice immunized with bivalent Delta/BA.1 vaccine had high VNTs against the pseudotyped Wuhan-Hu-1, Delta, and BA.1 strains but low VNTs against BA.2 and BA.5 (p < 0.05). Serum samples from mice immunized with Delta/BA.2 vaccine had high VNTs against the pseudotyped Wuhan-Hu-1, Delta, BA.1 and BA.2 strains but low VNTs against BA.5. Finally, serum samples from mice immunized with Delta/BA.5 vaccine had high VNTs against all the tested pseudotyped SARS-CoV-2 strains including the Wuhan-Hu-1, Delta, and Omicron variants (p > 0.05). Therefore, a bivalent mRNA vaccine with Delta/BA.5 combination is promising to provide broad spectrum immunity against all VOCs.
Effects of a Multimodal Approach Using Buprenorphine with/without Meloxicam on Food Intake, Body Weight, Nest Consolidating Behavior, Burrowing Behavior, and Gastrointestinal Tissues in Postoperative Male Mice
Authors: Furumoto Kayo , Sasaki Yuka , Nohara Masakatsu , Takenaka Nagisa , Maeta Noritaka , Kanda Teppei
Published: October 2022

Distress affects animal welfare and scientific data validity. There is a lack of reports on the effects of multimodal analgesic approaches in mice. In this study, under the hypothesis that a multimodal analgesic protocol using buprenorphine with meloxicam has analgesic effects, we evaluated the effects of a multimodal analgesic protocol using buprenorphine with meloxicam on the well-being of mice during analgesic administration by changing the dosage of meloxicam. A total of 42 Slc:ICR male mice were categorized into nonsurgical and surgical groups (7 mice per group) and treated with an anesthetic (isoflurane) and analgesics (buprenorphine ± meloxicam). Analgesics were administered for 48 h after treatment. Buprenorphine (subcutaneous; 0.1 mg/kg/8 h) and meloxicam (subcutaneous; 0, 2.5, or 5 mg/kg/24 h) were administered twice. Body weight, food intake, nest consolidation score, and latency to burrow were evaluated. A significant decrease in food intake was observed 24 h after treatment, while a significant increase was observed 48 h post-treatment in all groups. Body weight showed a decreasing trend but was not significantly reduced. Furthermore, stomach, duodenum, and jejunum tissues showed no morphological abnormalities. Significant differences in burrow diving scores and the latency to burrow were observed between some groups, but these were not regarded as a consequence of the surgery and/or the meloxicam dose. When buprenorphine and meloxicam were combined, administering up to 5 mg/kg/day of meloxicam for 48 h to male mice after abdominal surgery had no significant negative effects on any tested parameters. In conclusion, a multimodal analgesic protocol of buprenorphine with meloxicam is among the options for increasing well-being in mice following abdominal surgery.
Research on the Sustainable Development of the Bistrita Ardeleana River through the Resizing of Weirs
Authors: Moldovan Aurelian Cosmin , Micle Valer , Hrăniciuc Tomi Alexandrel , Marcoie Nicolae
Published: October 2022

The layout of water resources and the complex and rational use of them have an important role in the socioeconomic activities of an urban settlement. Transversal hydrotechnical constructions such as weirs reduce the longitudinal connectivity of rivers and streams, affecting river biodiversity as well as water quality. This paper presents an alternative method that will help restore connectivity. In order to choose the best solution, topographical measurements were taken with a total topographical station, and a professional drone was used to obtain an updated orthophoto plan. After processing the data obtained in the field, engineering software capable of simulating flow, sediment transport, and water quality in rivers was used. With the help of the software, two hypotheses were realized: hypothesis A, the case with only one weir in place, and hypothesis B, when we have the proposed case with the lowering of the height of the weir’s crest and the construction of three new control weirs downstream of it. In conclusion, the lowering of the current weir’s crest and the construction downstream of it of three new weirs of low height would have a favorable effect from an ecological and a morphological point of view, without very big consequences on the disturbance of the flow from a hydraulic point of view.
Assessing the Performance of SHETRAN Simulating a Geologically Complex Catchment
Authors: Vázquez Raúl F. , Brito Josué E. , Hampel Henrietta , Birkinshaw Stephen
Published: October 2022

Despite recent progress in terms of cheap computing power, the application of physically-based distributed (PBD) hydrological codes still remains limited, particularly, because some commercial-license codes are expensive, even under academic terms. Thus, there is a need for testing the performance of free-license PBD codes simulating complex catchments, so that cheap and reliable mechanistic modelling alternatives might be identified. The hydrology of a geologically complex catchment (586 km2) was modelled using the free-license PBD code SHETRAN. The SHETRAN evaluation took place by comparing its predictions with (i) discharge and piezometric time series observed at different locations within the catchment, some of which were not taken into account during model calibration (i.e., multi-site test); and (ii) predictions from a comparable commercial-license code, MIKE SHE. In general, the discharge and piezometric predictions of both codes were comparable, which encourages the use of the free-license SHETRAN code for the distributed modelling of geologically complex systems.
Performance Management of Natural Resources: A Systematic Review and Conceptual Framework for China
Authors: Zhang Zhengyu , Jin Gui , Hu Yihang , He Nianci , Niu Jinye
Published: October 2022

In recent decades, the issue of “Performance management of natural resources” has received increasing attention. To explore the optimization of performance management of natural resources is of great significance to the sustainable development of a country’s society and economy. Based on the relevant literature of “Performance management of natural resources” and “performance management and evaluation of nature resources” in Web of Science from 1990 to 2021, this study reviews the research progress of performance management of natural resources (including water resources) with the help of the CiteSpace V. Through literature review and inductive analysis, the authors found that the pursuit of sustainable utilization and management of natural resources has become the frontier direction of research. However, performance management of natural resources still lacks a general conceptual interpretation and analysis framework, and its evaluation system and methods still need to be further improved. The existing research on influencing factors of natural resources management performance still lacks depth, and the application of quantitative models needs to be strengthened in the future. The combination of research and quantitative models also needs to be further strengthened. Based on the existing literature and the practical experience of countries all over the world, this study constructs the research framework of performance management of natural resources for China. On the basis of multiple evaluation objectives, subjects and means, the authors describes the process and mechanism of performance management of natural resources, and gives some feasible evaluation methods for the performance management of natural resources, in order to provide decision support for the sustainable utilization of natural resources for China.
Rapid Response of Runoff Carrying Nitrogen Loss to Extreme Rainfall in Gentle Slope Farmland in the Karst Area of SW China
Authors: Wang Yong , Dai Quanhou , Ding Pengwei , Li Kuaifen , Yi Xingsong , He Jie , Peng Xudong , Yan Youjin , Zhao Min , Yang Yingchong
Published: October 2022

Nitrogen loss is the main reason for land quality degradation and productivity decline and an important factor in groundwater pollution. Extreme rainfall has occurred frequently in Karst areas of southwest China in recent years. It is of great significance to study the response of soil nitrogen loss to extreme rainfall in Karst areas to prevent and treat land quality degradation and non-point source pollution. In this study, field monitoring and indoor artificial rainfall simulation were used to study the loss characteristics of total soil nitrogen (TN), ammonium (NH4+-N) nitrogen, and nitrate-nitrogen (NO3−-N) in Karst bare slope farmland (slope angles of 5° and 10°) under extreme rainfall conditions. The results showed that: (1) Extreme rainfall (90 mm/h) increased the surface runoff, middle soil runoff, and underground runoff by 1.68 times, 1.16 times, and 1.43 times, respectively, compared with moderate rainfall (60 mm/h), so that nitrogen loss increased with runoff. (2) The loss of nitrate-nitrogen in surface, soil, and underground under extreme rainfall conditions was 223.99, 147.93, and 174.02% higher than that under moderate rainfall conditions, respectively; the nitrate losses were 203.78, 160.18, and 195.39% higher, respectively. Total nitrogen losses were 187.33, 115.45, and 138.68% higher, respectively. (3) The influencing factors of total soil nitrogen and nitrate-nitrogen loss in Karst slope farmland were slope > rainfall duration > rainfall intensity, while the influencing factors of ammonium nitrogen loss were rainfall duration > slope > rainfall intensity. Therefore, in controlling nitrogen loss in Karst slope farmland, changing slope degree and increasing farmland coverage may be useful measures to slow the nitrogen loss caused by extreme rainfall.
Systems Thinking for Planning Sustainable Desert Agriculture Systems with Saline Groundwater Irrigation: A Review
Authors: Shin Sangmin , Aziz Danyal , El-sayed Mohamed E. A. , Hazman Mohamed , Almas Lal , McFarland Mike , El Din Ali Shams , Burian Steven J.
Published: October 2022

Agricultural land expansion is a solution to address global food security challenges in the context of climate change. However, the sustainability of expansion in arid countries is difficult because of scarce surface water resources, groundwater salinity, and the health of salt-affected soil. Developing expansion and sustainability plans for agriculture requires systems thinking, considering the complex feedback interactions between saline groundwater, salt-affected soil, plant growth, freshwater mixing with saline groundwater, irrigation systems, and the application of soil amendments to alleviate the salinity impacts. This study presents an extensive literature review on the effects of salinity on soil and plant health, the constraints and opportunities for sustainable agriculture in Egypt, and a systems thinking approach to the feedback interactions between saline water, salt-affected soil, and the application of soil amendments to achieve required crop yields. Insights and strategies are discussed, including a system-dynamics-based decision model, irrigation systems with diversified and decentralized water sources, urban water demand management, energy availability, smart irrigation systems, and active participation of stakeholders to achieve sustainable agriculture under climate and socioeconomic changes. The insights are expected to encourage stakeholders and academic communities in the water, agriculture, and related food security sectors to develop a quantitative and systematic decision-making framework for sustainable agriculture systems in arid regions.