bluuebunny/crossref_metadata_2025 huggingface dataset:
Non-Solvent Induced Phase Separation (NIPS) for Fabricating High Filtration Efficiency (FE) Polymeric Membranes for Face Mask and Air Filtration Applications
Authors: Ogbuoji Ebuka A. , Stephens Lauren , Haycraft Amber , Wooldridge Eric , Escobar Isabel C.
Published: June 2022

Protection against airborne viruses has become very relevant since the outbreak of SARS-CoV-2. Nonwoven face masks along with heating, ventilation, and air conditioning (HVAC) filters have been used extensively to reduce infection rates; however, some of these filter materials provide inadequate protection due to insufficient initial filtration efficiency (FE) and FE decrease with time. Flat sheet porous membranes, which have been used extensively to filter waterborne microbes and particulate matter due to their high FE have the potential to filter air pollutants without compromising its FE over time. Therefore, in this study, single layer polysulfone (PSf) membranes were fabricated via non-solvent induced phase separation (NIPS) and were tested for airflow rate, pressure drop and FE. Polyethylene glycol (PEG) and glycerol were employed as pore-forming agents, and the effect of the primary polymer and pore-forming additive molecular weights (MW) on airflow rate and pressure drop were studied at different concentrations. The thermodynamic stability of dope solutions with different MWs of PSf and PEG in N-methylpyrrolidone (NMP) at different concentrations was determined using cloud-point measurements to construct a ternary phase diagram. Surface composition of the fabricated membranes was characterized using contact angle and X-ray photoelectron spectroscopy (XPS), while membrane morphology was characterized by SEM, and tensile strength experiments were performed to analyze the membrane mechanical strength (MS). It was observed that an increase in PSf and PEG molecular weight and concentration increased airflow and decreased pressure drop. PSf60:PEG20:NMP (15:15:70)% w/w showed the highest air flow rate and lowest pressure drop, but at the expense of the mechanical strength, which was improved significantly by attaching the membrane to a 3D-printed polypropylene support. Lastly, the FE values of the membranes were similar to those of double-layer N95 filters and significantly higher than those of single layer of N95, surgical mask and HVAC (MERV 11) filters.
Wicking in Porous Polymeric Membranes: Determination of an Effective Capillary Radius to Predict the Flow Behavior in Lateral Flow Assays
Authors: Altschuh Patrick , Kunz Willfried , Bremerich Marcel , Reiter Andreas , Selzer Michael , Nestler Britta
Published: June 2022

The working principle of lateral flow assays, such as the widely used COVID-19 rapid tests, is based on the capillary-driven liquid transport of a sample fluid to a test line using porous polymeric membranes as the conductive medium. In order to predict this wicking process by simplified analytical models, it is essential to determine an effective capillary radius for the highly porous and open-pored membranes. In this work, a parametric study is performed with selected simplified structures, representing the complex microstructure of the membrane. For this, a phase-field approach with a special wetting boundary condition to describe the meniscus formation and the corresponding mean surface curvature for each structure setup is used. As a main result, an analytical correlation between geometric structure parameters and an effective capillary radius, based on a correction factor, are obtained. The resulting correlation is verified by applying image analysis methods on reconstructed computer tomography scans of two different porous polymeric membranes and thus determining the geometric structure parameters. Subsequently, a macroscale flow model that includes the correlated effective pore size and geometrical capillary radius is applied, and the results are compared with wicking experiments. Based on the derived correction function, it is shown that the analytical prediction of the wicking process in highly porous polymeric membranes is possible without the fitting of experimental wicking data. Furthermore, it can be seen that the estimated effective pore radius of the two membranes is 8 to 10 times higher than their geometric mean pore radii.
Study on Shear Strength of Partially Connected Steel Plate Shear Wall
Authors: Yang Yuqing , Mu Zaigen , Zhu Boli
Published: June 2022

The paper proposes partially connected steel plate shear walls, in which the infill plates and frames are connected by discretely distributed fish plates at the corners and at the centers. The high lateral resistance of a steel plate shear wall has led to its widespread use in the design of structural shear resistance. In this paper, finite element models of the partially connected steel plate shear walls are established by the finite element method, and the effect of the different partial connections on the shear strength is firstly investigated. Moreover, the variation of the shear strength with the plate-to-frame connectivity ratio is analyzed numerically, and the effect of the connectivity ratio on the development of the tensile field is studied. Based on the numerical analysis results, the effect of the connectivity ratio on shear strength is evident at low levels. When the connectivity ratio is over 80%, the shear strength of the partially connected steel plate shear wall can reach 95% of that of the fully connected steel plate shear wall. When the connection ratio is at a low level, the advantages of the central connection on the shear strength of the structures are higher than those with corner connections. Furthermore, the fitting formula for the partially connected steel plate shear wall is obtained by changing the connectivity ratio and width-to-height ratio of the examples, which can predict the shear capacity of the partially connected steel plate shear wall with different partial connections.
Fatigue Crack Initiation on Semi-Solid Al–7Si–Mg Castings
Authors: Santos Jorge , Zhu Baiwei , Zanella Caterina , Jarfors Anders E. W.
Published: June 2022

Four-point bending fatigue tests were performed on semi-solid Al–7Si–Mg castings with varying magnesium contents and heat treatment conditions. Additionally, the effect of anodising on the fatigue resistance of semi-solid Al–7Si–Mg castings was evaluated. Fracture surface and microstructure analysis showed that fatigue crack initiation occurred mainly at the periphery of regions of positive macrosegregation at the casting surface, resulting most likely from exudation. The microstructure of these macrosegregation regions was mostly eutectic and was frequently found surrounded by a layer of oxides. This layer of oxides promoted weak bonding between the macrosegregation region and the surrounding material and acted as a crack initiation site. In this study, primary α-Al globule agglomerates at the casting surface and surrounded by a layer of oxides also promoted fatigue crack initiation. Fatigue resistance of semi-solid Al–7Si–Mg castings in the T5 and T6 conditions increased with the increase in the magnesium content of the alloy from 0.3 to 0.45 wt.% due to the higher precipitation hardening response. However, the increase in the magnesium content from 0.45 to 0.6 wt.% resulted in a slight decrease in the fatigue resistance. The oxide layer formed during anodising had no significant effect on the fatigue resistance of the semi-solid Al–7Si–Mg castings in this study due to the dominant effect of the macrosegregation regions on fatigue crack initiation.
Influence of Partial Rust Layer on the Passivation and Chloride-Induced Corrosion of Q235b Steel in the Carbonated Simulated Concrete Pore Solution
Authors: Li Kaiqiang , Li Xincheng , Zhao Yubin , Wang Kangchen , Song Shenyou , Jin Wenliang , Xia Dahai , Xu Yunze , Huang Yi
Published: June 2022

A partial pre-rusted wire beam electrode (WBE) was designed to study the influence of the rust layer on rebar corrosion in the carbonated simulated concrete pore solution (SCPS). The results show that the passive film generated on the pre-rusted steel area is more fragile than that formed on the fine polished steel area in carbonaceous media. Nevertheless, the pitting corrosion resulting from the presence of chloride ions still tends to occur on the fine polished steel surface due to the local acidification process being hindered by the rust layer. The rust layer could play a more important role than the passive film in inhibiting the initiation of chloride-induced corrosion on rebar. The expansion path of the corrosion product would be blocked by the rust layer, leading to the pit propagating in the fine polished region. Furthermore, the growth of pitting corrosion is greatly accelerated due to the catalytic cathodic reaction of the rust layer.
Study on Wavelet Packet Energy Characteristics on Friction Signal of Lapping with the Fixed Abrasive Pad
Authors: Wang Zhankui , Zhang Zhao , Wang Shiwei , Pang Minghua , Ma Lijie , Su Jianxiu
Published: June 2022

The surface condition of the fixed abrasive pad (FAP) has a significant impact on its machining performance, workpiece material removal rate (MRR), and surface roughness. To clarify the wavelet packet energy characteristics of friction signal under different surface conditions of FAP and its mapping relationship with MRR and workpiece surface quality, FAP samples in different processing stages were obtained through a consolidated abrasive grinding quartz glass experiment. Then, the friction signals in different stages were received by the friction and wear experiment between the FAP and quartz glass workpiece, and the wavelet packet analysis was carried out. The experimental results show that with the increase of lapping time, the surface wear degree of the FAP increased gradually, and the MRR of the workpiece, the surface roughness of the FAP, and the surface roughness of the workpiece decreased slowly. In the wavelet packet energy of friction signal during machining, the energy proportion of frequency band 7 showed an upward trend with the increase of lapping time. The energy proportion of frequency band 8 showed a downward trend with the increase of lapping time. The change characteristics of the two are significantly correlated with the surface condition of the FAP.
The Emergence of Carbapenem-Resistant Gram-Negative Bacteria in Mizoram, Northeast India
Authors: Ralte Vanlalruati S. C. , Loganathan Archana , Manohar Prasanth , Sailo Christine Vanlalbiakdiki , Sanga Zothan , Ralte Lalremruata , Zothanzama John , Leptihn Sebastian , Nachimuthu Ramesh , Kumar Nachimuthu Senthil
Published: June 2022

(Background) Numerous reports on carbapenem resistance in different parts of India have been published, yet there are insufficient studies on the prevalence of antibiotic resistance in the northeast region of the country. This study evaluated the emergence of carbapenem resistance in the clinical isolates collected in Mizoram. (Results) A total of 141 Gram-negative clinical isolates were collected from the two hospitals, including the Civil Hospital in Lunglei and the Synod Hospital in Aizawl. The isolates include Escherichia coli (n = 62, 43.9%), Klebsiella spp. (n = 43, 30.4%), Pseudomonas aeruginosa (n = 9, 6.3%), Serratia marcescens (n = 3, 2.1%), Proteus mirabilis (n = 2, 1.4%), Shigella spp. (n = 4, 2.8%), Enterobacter spp. (n = 6, 4.2%) and Acinetobacter spp. (n = 12, 8.5%). The isolates were found to be resistant to meropenem (11%), colistin (48%), tigecycline (25%) and cefotaxime (50%). A total of four E. coli and one Shigella sonnei encoded the blaOXA-48-like gene. The blaCTX-M-1 gene was detected in 13 isolates, of which eight were E. coli, two Shigella flexneri, and one isolates each of K. pneumoniae, K. oxytoca and Shigella sonnei, respectively. (Conclusion) Carbapenem-resistant Enterobacteriaceae are common among other parts of India, despite limited access to antibiotics, the emergence of resistance in the northeastern region is worrying.
Kingella kingae Reveals Its Secrets
Authors: Yagupsky Pablo
Published: June 2022

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Sulfide and Fluoride Mineralization of the NNE Region of Achemmach (Central Morocco): Paragenetic Sequences and Pyrrhotite-Sphalerite Geothermometry Constraints
Authors: Mezougane Hafid , Aissa Mohamed , Muhammad Souiri , Moussaid Azizi , El Basbas Abdelaziz , Essalhi Mourad , Kharis Abdel-ali , El Azmi Mohammed , Touil Ahmed , Bilal Essaid
Published: June 2022

Sulfide and fluoride mineralization in the NNE Achemmach (NNE-A) area is located in the NE of Central Hercynian Morocco. In veins or when disseminated, it is hosted either in Visean sedimentary formations or in the magmatic bodies, described for the first time in this article and corresponding to pillow-lavas, dolerites and olivine-bearing gabbros. The mineralization is multiphase and results from the succession of the following three events: (i) an early high-temperature hydrothermal event (T ≈ 350–420 °C) associated with a simple primary sulfide paragenesis composed of pyrrhotite, pyrite, chalcopyrite, sphalerite and galena with gangue of quartz.(ii) The second event corresponds witha low temperature fluorite hydrothermal one (T≈ 120–160 °C), whereas the (iii) third is marked by, the deposition of a late sulfide paragenesis in a carbonate gangue within a moderate temperature (T≈ 200–250 °C). The temperatures of the paragenetic stages (350–400 °C) are estimated on the basis of the geothermometry constraints of the mineralogical assemblages, particularly the pyrrhotite-sphalerite equilibrium, in which the FeO content varies from 9.23 to 14.42 Wt%, and in the full study of their corresponding fluid phases. They are in perfect agreement with the fluid inclusion data of the first event.
Geochronology and Tectonic Implications of the Nianzigou Granites and Associated Mo Deposit, Inner Mongolia
Authors: Li Yang , Yang Yongqiang , Hua Lei , Leng Yaxing , Xin Jiang
Published: June 2022

Multiple stages of igneous rocks occur in the recently discovered Nianzigou Mo deposit in Chifeng, Inner Mongolia, which can provide insights into the late Mesozoic geodynamic evolution of the southern Da Hinggan Range. The mineralization age is similar to the age of local granites, but there are few detailed studies of the tectonic setting during Cu-Mo mineralization in this area. The Nianzigou Mo deposit is located close to the northern margin of the North China Craton and in the eastern Central Asian Orogenic Belt and is a typical quartz-vein-type Mo deposit in the Xilamulun Mo ore belt. The granite in this deposit has high SiO2, Al2O3, K2O, and Na2O contents, and low MgO, CaO, and Fe2O3t contents. The granite is characterized by enrichments in large-ion lithophile elements and depletions in high-field-strength elements and, in particular, Sr, Ti, and P. The granite has high contents of rare-earth elements, is enriched in light rare-earth elements, and has marked negative Eu anomalies. The granite is an alkaline and calc-alkaline and metaluminous A-type granite. The zircon U-Pb ages of the monzogranite and granite porphyry are 157.2 ± 0.3 and 154.4 ± 0.4 Ma. The model age obtained by Re-Os isotopic dating is 154.3 ± 1.7 Ma, indicating that molybdenite mineralization also occurred during the Late Jurassic period. Given that the molybdenite Re contents are 7.8–24.9 ppm (average = 16.8 ppm), the ore-forming materials of the Nianzigou Mo deposit had a mixed crust–mantle source, but were mainly derived from the lower crust. Based on the geology and geochemistry, we propose that the Nianzigou Mo deposit formed in a postorogenic extensional tectonic setting associated with the southward subduction of the Mongol–Okhotsk oceanic plate.
Determination of the Phenolic Profile by Liquid Chromatography, Evaluation of Antioxidant Activity and Toxicity of Moroccan Erica multiflora, Erica scoparia, and Calluna vulgaris (Ericaceae)
Authors: Bekkai Douaa , Oulad El Majdoub Yassine , Bekkai Hamid , Cacciola Francesco , Miceli Natalizia , Taviano Maria Fernanda , Cavò Emilia , Errabii Tomader , Laganà Vinci Roberto , Mondello Luigi , L’Bachir El Kbiach Mohammed
Published: June 2022

This study aimed to investigate the phenolic profile and selected biological activities of the leaf and aerial extracts of three Ericaceae species, namely Erica multiflora, Erica scoparia, and Calluna vulgaris, collected from three different places in the north of Morocco. The phenolic composition of all extracts was determined by LC coupled with photodiode array and mass spectrometry detection. Among the investigated extracts, that of E. scoparia aerial parts was the richest one, with a total amount of polyphenols of 9528.93 mg/kg. Up to 59 phenolic compounds were detected: 52 were positively identified and 49 quantified—11 in C. vulgaris, 14 in E. multiflora, and 24 in E. scoparia. In terms of chemical classes, nine were phenolic acids and 43 were flavonoids, and among them, the majority belonged to the class of flavonols. The antioxidant activity of all extracts was investigated by three different in vitro methods, namely DPPH, reducing power, and Fe2+ chelating assays; E. scoparia aerial part extract was the most active, with an IC50 of 0.142 ± 0.014 mg/mL (DPPH test) and 1.898 ± 0.056 ASE/mL (reducing power assay). Further, all extracts were non-toxic against Artemia salina, thus indicating their potential safety. The findings attained in this work for such Moroccan Ericaceae species, never investigated so far, bring novelty to the field and show them to be valuable sources of phenolic compounds with interesting primary antioxidant properties.
Graphene Metamaterial Embedded within Bundt Optenna for Ultra-Broadband Infrared Enhanced Absorption
Authors: Awad Ehab
Published: June 2022

Graphene is well-known for its extraordinary physical properties such as broadband optical absorption, high electron mobility, and electrical conductivity. All of these make it an excellent candidate for several infrared applications such as photodetection, optical modulation, and optical sensing. However, a standalone monolayer graphene still suffers from a weak infrared absorption, which is ≅2.3%. In this work, a novel configuration of graphene metamaterial embedded inside Bundt optical-antenna (optenna) is demonstrated. It can leverage the graphene absorption up to 57.7% over an ultra-wide wavelength range from 1.26 to 1.68 µm (i.e., Bandwidth ≅ 420 nm). This range covers the entire optical communication bands of O, E, S, C, L, and U. The configuration mainly consists of a Bundt-shaped plasmonic antenna with a graphene metamaterial stack embedded within its nano-wide waveguide that has a 1.5 µm length. The gold average plasmonic loss is ≅25%. This configuration can enhance graphene ultra-broadband absorption through multiple mechanisms. It can nano-focus the infrared radiation down to a 50 nm spot on the graphene metamaterial, thus yielding an 11.5 gain in optical intensity (i.e., 10.6 dB). The metamaterial itself has seven concentric cylindrical graphene layers separated by silicon dioxide thin films, thus each layer contributes to the overall absorption. The focused infrared propagates tangential to the graphene metamaterial layers (i.e., grazing propagation), and thus maximizes the light–graphene interaction length. In addition, each graphene layer experiences a double-face exposure to the nano-focused propagating spot, which increases each layer’s absorption. This configuration is compact and polarization-insensitive. The estimated maximum absorption enhancement compared to the standalone monolayer graphene was 25.1 times (i.e., ≅4 dB). The estimated maximum absorption coefficient of the graphene stack was 5700 cm−1, which is considered as one of the record-high reported coefficients up to date.
Towards Perfect Ultra-Broadband Absorbers, Ultra-Narrow Waveguides, and Ultra-Small Cavities at Optical Frequencies
Authors: Goudarzi Kiyanoush , Lee Moonjoo
Published: June 2022

In this study, we design ultra-broadband optical absorbers, ultra-narrow optical waveguides, and ultra-small optical cavities comprising two-dimensional metallic photonic crystals that tolerate fabrication imperfections such as position and radius disorderings. The absorbers containing gold rods show an absorption amplitude of more than 90% under 54% position disordering at 200<λ<530 nm. The absorbers containing silver rods show an absorptance of more than 90% under 54% position disordering at 200<λ<400 nm. B-type straight waveguides that contain four rows of silver rods exposed to air reveal normalized transmittances of 75% and 76% under 32% position and 60% radius disorderings, respectively. B-type L-shaped waveguides containing four rows of silver rods show 76% and 90% normalized transmittances under 32% position and 40% radius disorderings, respectively. B-type cavities containing two rings of silver rods reveal 70% and 80% normalized quality factors under 32% position and 60% radius disorderings, respectively.
An Ultrathin Tunable Metamaterial Absorber for Lower Microwave Band Based on Magnetic Nanomaterial
Authors: Ning Jing , Chen Ke , Zhao Wenbo , Zhao Junming , Jiang Tian , Feng Yijun
Published: June 2022

At frequencies below 1 GHz, conventional microwave absorbers are limited by their large thickness or narrow absorption bandwidth; therefore, new techniques for efficient absorption for the lower microwave band are highly demanded. Here, we propose and fabricate an ultrathin tunable metamaterial absorber combining magnetic nanomaterials and metamaterial resonant structures for use in the lower microwave band (P band). The proposed absorber utilizes electrically controlled varactors to enable frequency tunability and magnetic nanomaterials as dielectric slabs for thickness reduction and bandwidth expansion at low frequencies. By adjusting the bias voltages of varactors, the resonant behavior of the absorbing structure can be dynamically tuned that covers a continuously tunable absorbing band from 0.41 to 1.02 GHz (85.3% in fractional bandwidth) with at least 10 dB reflection reduction. The total thickness of this absorber is 5 mm, which is only about 1/146 the wavelength of the lowest frequency. The agreement between the simulated and measured results validates the proposed design, and the structure has good angular stability that may be used as complex targets for low-RCS applications.
Silicon-Based Metastructure Optical Scattering Multiply–Accumulate Computation Chip
Authors: Liu Xu , Zhu Xudong , Wang Chunqing , Cao Yifan , Wang Baihang , Ou Hanwen , Wu Yizheng , Mei Qixun , Zhang Jialong , Cong Zhe , Liu Rentao
Published: June 2022

Optical neural networks (ONN) have become the most promising solution to replacing electronic neural networks, which have the advantages of large bandwidth, low energy consumption, strong parallel processing ability, and super high speed. Silicon-based micro-nano integrated photonic platforms have demonstrated good compatibility with complementary metal oxide semiconductor (CMOS) processing. Therefore, without completely changing the existing silicon-based fabrication technology, optoelectronic hybrid devices or all-optical devices of better performance can be achieved on such platforms. To meet the requirements of smaller size and higher integration for silicon photonic computing, the topology of a four-channel coarse wavelength division multiplexer (CWDM) and an optical scattering unit (OSU) are inversely designed and optimized by Lumerical software. Due to the random optical power splitting ratio and incoherency, the intensities of different input signals from CWDM can be weighted and summed directly by the subsequent OSU to accomplish arbitrary multiply–accumulate (MAC) operations, therefore supplying the core foundation for scattering ONN architecture.
Vitamin D and Risk of Obesity-Related Cancers: Results from the SUN (‘Seguimiento Universidad de Navarra’) Project
Authors: Sánchez-Bayona Rodrigo , Bes-Rastrollo Maira , Fernández-Lázaro Cesar I. , Bastyr Maite , Madariaga Ainhoa , Pons Juan J. , Martínez-González Miguel A. , Toledo Estefanía
Published: June 2022

Obesity is associated with a higher risk of several types of cancer, grouped as obesity-related cancers (ORC). Vitamin D deficiency is more prevalent in obese subjects, and it has been suggested to play a role in the association between obesity and cancer risk. The aim of the study was to analyze the association between vitamin D intake and the subsequent risk of ORC in a prospective Spanish cohort of university graduates. The SUN Project, initiated in 1999, is a prospective dynamic multipurpose cohort. Participants answered a 556-item lifestyle baseline questionnaire that included a validated food-frequency questionnaire. We performed Cox regression models to estimate the hazard ratios (HRs) of ORC according to quartiles of energy-adjusted vitamin D intake (diet and supplements). We included 18,017 participants (mean age = 38 years, SD = 12 years), with a median follow-up of 12 years. Among 206,783 person-years of follow-up, we identified 225 cases of ORC. We found no significant associations between vitamin D intake and ORC risk after adjusting for potential confounders: HRQ2vsQ1 = 1.19 (95% CI 0.81–1.75), HRQ3vsQ1 = 1.20 (95% CI 0.81–1.78), and HRQ4vsQ1 = 1.02 (95% CI 0.69–1.51). Dietary and supplemented vitamin D do not seem to be associated with ORC prevention in the middle-aged Spanish population.
The Prevalence and Risk Factors Associated with Iodine Deficiency in Canadian Adults
Authors: Mathiaparanam Stellena , Nori de Macedo Adriana , Mente Andrew , Poirier Paul , Lear Scott A. , Wielgosz Andreas , Teo Koon K. , Yusuf Salim , Britz-Mckibbin Philip
Published: June 2022

Iodine is a trace micronutrient that is critical for normal thyroid function and human health. Inadequate dietary intake is associated with cognitive impairment, infertility, growth retardation and iodine deficiency disorders in affected populations. Herein, we examined the prevalence of iodine deficiency in adults (median age of 61 years) based on the analysis of 24 h urine samples collected from 800 participants in four clinical sites across Canada in the Prospective Urban and Rural Epidemiological (PURE) study. Urinary iodide together with thiocyanate and nitrate were measured using a validated capillary electrophoresis assay. Protective/risk factors associated with iodine deficiency were identified using a binary logistic regression model, whereas daily urinary iodine concentration (24 h UIC, μg/L) and urinary iodine excretion (24 h UIE, μg/day) were compared using complementary statistical methods with covariate adjustments. Overall, our Canadian adult cohort had adequate iodine status as reflected by a median UIC of 111 μg/L with 11.9% of the population <50 μg/L categorized as having moderate to severe iodine deficiency. Iodine adequacy was also evident with a median 24 h UIE of 226 μg/day as a more robust metric of iodine status with an estimated average requirement (EAR) of 7.1% (< 95 μg/day) and a tolerable upper level (UL) of 1.8% (≥1100 μg/day) based on Canadian dietary reference intake values. Participants taking iodine supplements (OR = 0.18; p = 6.35 × 10−5), had greater 24 h urine volume (OR = 0.69; p = 4.07 × 10−4), excreted higher daily urinary sodium (OR = 0.71; p = 3.03 × 10−5), and/or were prescribed thyroxine (OR = 0.33; p = 1.20 × 10−2) had lower risk for iodine deficiency. Self-reported intake of dairy products was most strongly associated with iodine status (r = 0.24; p = 2.38 × 10−9) after excluding for iodine supplementation and T4 use. Participants residing in Quebec City (OR = 2.58; p = 1.74 × 10−4) and Vancouver (OR = 2.54; p = 3.57 × 10−4) were more susceptible to iodine deficiency than Hamilton or Ottawa. Also, greater exposure to abundant iodine uptake inhibitors from tobacco smoking and intake of specific goitrogenic foods corresponded to elevated urinary thiocyanate and nitrate, which were found for residents from Quebec City as compared to other clinical sites. Recent public health policies that advocate for salt restriction and lower dairy intake may inadvertently reduce iodine nutrition of Canadians, and further exacerbate regional variations in iodine deficiency risk.
Review on Bovine Tuberculosis: An Emerging Disease Associated with Multidrug-Resistant Mycobacterium Species
Authors: Borham Mohamed , Oreiby Atef , El-Gedawy Attia , Hegazy Yamen , Khalifa Hazim O. , Al-Gaabary Magdy , Matsumoto Tetsuya
Published: June 2022

Bovine tuberculosis is a serious infectious disease affecting a wide range of domesticated and wild animals, representing a worldwide economic and public health burden. The disease is caused by Mycobacteriumbovis and infrequently by other pathogenic mycobacteria. The problem of bovine tuberculosis is complicated when the infection is associated with multidrug and extensively drug resistant M. bovis. Many techniques are used for early diagnosis of bovine tuberculosis, either being antemortem or postmortem, each with its diagnostic merits as well as limitations. Antemortem techniques depend either on cellular or on humoral immune responses, while postmortem diagnosis depends on adequate visual inspection, palpation, and subsequent diagnostic procedures such as bacterial isolation, characteristic histopathology, and PCR to reach the final diagnosis. Recently, sequencing and bioinformatics tools have gained increasing importance for the diagnosis of bovine tuberculosis, including, but not limited to typing, detection of mutations, phylogenetic analysis, molecular epidemiology, and interactions occurring within the causative mycobacteria. Consequently, the current review includes consideration of bovine tuberculosis as a disease, conventional and recent diagnostic methods, and the emergence of MDR-Mycobacterium species.
Synthesis of Prussian Blue Nanoparticles and Their Antibacterial, Antiinflammation and Antitumor Applications
Authors: Li Danyang , Liu Meng , Li Wenyao , Fu Qiang , Wang Liyang , Lai Enping , Zhao Weixin , Zhang Kaile
Published: June 2022

In recent years, Prussian blue nanoparticles (PBNPs), also named Prussian blue nano-enzymes, have been shown to demonstrate excellent multi-enzyme simulation activity and anti-inflammatory properties, and can be used as reactive oxygen scavengers. Their good biocompatibility and biodegradability mean that they are ideal candidates for in vivo use. PBNPs are highly efficient electron transporters with oxidation and reduction activities. PBNPs also show considerable promise as nano-drug carriers and biological detection sensors owing to their huge specific surface area, good chemical characteristics, and changeable qualities, which might considerably increase the therapeutic impact. More crucially, PBNPs, as therapeutic and diagnostic agents, have made significant advances in biological nanomedicine. This review begins with a brief description of the synthesis methods of PBNPs, then focuses on the applications of PBNPs in tissue regeneration and inflammation according to the different properties of PBNPs. This article will provide a timely reference for further study of PBNPs as therapeutic agents.
Genetic Diversity and Pedigree Analysis of Red Currant Germplasm
Authors: Pikunova Anna , Goryunova Svetlana , Goryunov Denis , Golyaeva Olga , Dolzhikova Maria , Pavlenko Anna
Published: June 2022

This represents the first report on the genetic diversity of red currant germplasm collections based on genotyping-by-sequencing (GBS) data. Genotypes of 75 individuals of different origin were assessed in more than 7.5K genome positions. Multidimensional scaling (MDS) analysis has been performed. There are five accessions that are significantly isolated from each other and from the rest of the analyzed cultivars. F1 offspring of R. petraeum Wulf (Rote Hollandische) and Gondouin, as well as Rote Spatlese (F2 of R. petraeum and F2 of R. multiflorum Kit.), are the most genetically isolated on the MDS plot. Ribes multiflorum is closer to the rest of cultivars than the three abovementioned accessions. Purpurnaya cultivar (F1 of Rote Spatlese) is located between Rote Hollandische and R. multiflorum. Other genotypes, mostly represented by varieties having several species in a pedigree, occupied the rest of MDS plot relatively evenly. Descendants of R. multiflorum have been placed in the left part of MDS plot, which underlines their genetic diversity from other accessions. White- and pink-fruited cultivars were clustered together, underlining genetic relatedness. Admixture analysis of GBS data reveals six clusters (K = 6). Presumably, clustering reflects relatedness to R. petraeum, R. rubrum, R. vulgare var macrocarpum, R. multiflorum, R. vulgare, and Jonker van Tets. Based on genotyping data, F1 offspring of R. warscewiczs Jancz (cultivar Viksne), R. altissimum Turcz (Cirald), and R. palczewskii (Jancz.) Pojark (Skorospelaya) have not exhibited strict separation and were placed in a pool with other varieties. This supports modern taxonomic classifications that do not consider R. altissimum and R. palczewskii as independent species.
Assessment of Vegetative Growth and Genetic Integrity of Vanilla planifolia Regenerants after Cryopreservation
Authors: González-Arnao María Teresa , Cruz-Cruz Carlos A. , Hernández-Ramírez Fabiola , Alejandre-Rosas Jorge A. , Hernández-Romero Ana Constanza
Published: June 2022

Vanilla planifolia Jacks. ex Andrews is the vanilla species with the most commercial and greatest economic importance. It has been used as a case study in different cryopreservation studies that involve three vitrification-based approaches: droplet-vitrification (D-V), V-cryoplate (V-Cp) and D-cryoplate (D-Cp). The aim of this study was to compare the impact of these cryogenic techniques on vegetative growth (survival, stem length and leaf number) between cryo-derived plants and in vitro-derived controls during 12 months of greenhouse growth. Genetic stability was also assessed using the inter-simple sequence repeat (ISSR) markers. There were no significant differences found in the survival and stem lengths of the in vitro-derived regenerants and cryo-derived plants. A significant increase in the number of leaves was only detected in cryo-derived plants when using the V-Cp method. The electrophoretic profiles, based on seven ISSR primers, detected low variability: 81 total bands and 27% polymorphism. This is the first report on the assessment of vegetative growth and genetic integrity in cryo-derived V. planifolia plants recovered under greenhouse conditions. Of the three cryogenic approaches, D-Cp appears to yield V. planifolia regenerants plants with more vigorous vegetative growth and a lower level of polymorphism. Future research should focus on the reproductive growth of vanilla regenerants.
Genome-Wide Identification and Structural Characterization of Growth-Regulating Factors (GRFs) in Actinida eriantha and Actinidia chinensis
Authors: Abid Muhammad , Wang Zupeng , Feng Chen , Luo Juan , Zhang Yi , Tu Jing , Cai Xinxia , Gao Puxin
Published: June 2022

Growth-regulating factors (GRFs) encode plant-specific transcription factors that play a vital role in regulation of plant growth, development, and stress response. Although GRFs have been identified in various plants, there is no reported work available in Actinidia (commonly known as kiwifruit) so far. In the present study, we identified 22 GRF genes on A. chinensis (hereafter A. chinensis is referred to as Ac, and GRF genes in A. chinensis are referred to as AcGRF) distributed on 17 chromosomes and one contig, and 26 GRF genes in A. eriantha (hereafter A. eriantha is referred to as Ae, and GRF genes in A. eriantha are referred to as AeGRF) distributed on 21 chromosomes. Phylogenetic analysis showed that kiwifruit GRF proteins were clustered into five distinct groups. Additionally, kiwifruit GRFs showed motif composition and gene structure similarities within the same group. Synteny analysis showed that whole-genome duplication played a key role in the expansion of the GRF family in kiwifruit. The higher expression levels of kiwifruit GRFs in young tissues and under stress conditions indicated their regulatory role in kiwifruit growth and development. We observed two genes in Ae (AeGRF6.1, AeGRF 6.2) and two genes in Ac (AcGRF 6.1, AeGRF 6.2) significantly upregulated in different RNA-seq datasets. The presence of conserved protein structures and cis-regulatory elements caused functional divergence in duplicated gene pairs. The subcellular localization indicated the presence of kiwifruit GRFs in the nucleus of the plant cell. Protein-protein interaction analysis predicted AtGIF protein orthologs for AcGRFs and AeGRFs. Taken together, we systematically analyzed the characterization of kiwifruit GRF family members for their potential role in kiwifruit development and Pseudomonas syringae pv. actinidiae (Psa.) invasion response. Further functional studies of kiwifruit GRFs in plant growth, development, and stress response will provide valuable insights for kiwifruit breeders.
Comparative Transcriptome Analysis of Two Kalanchoë Species during Plantlet Formation
Authors: Jácome-Blásquez Francisco , Ooi Joo Phin , Zeef Leo , Kim Minsung
Published: June 2022

Few species in the Kalanchoë genus form plantlets on their leaf margins as an asexual reproduction strategy. The limited molecular studies on plantlet formation show that an organogenesis ortholog, SHOOTMERISTEMLESS (STM) and embryogenesis genes, such as LEAFY COTYLEDON1 (LEC1) and FUSCA3 are recruited during plantlet formation. To understand the mechanisms of two Kalanchoë plantlet-forming species with different modes of plantlet formation, RNA-sequencing analysis was performed. Differentially expressed genes between the developmental stages were clustered in K. daigremontiana (Raym.-Hamet and H. Perrier) and K. pinnata (Lam. Pers.), respectively. Of these gene clusters, GO terms that may be involved in plantlet formation of both species, such as signaling, response to wounding, reproduction, regulation of hormone level, and response to karrikin were overrepresented. Compared with the common GO terms, there were more unique GO terms overrepresented during the plantlet formation of each species. A more in-depth investigation is required to understand how these pathways are participating in plantlet formation. Nonetheless, this transcriptome analysis is presented as a reliable basis for future studies on plantlet formation and development in two Kalanchoë plantlet-forming species.
Characteristics of Small-Molecule Migration of Silicone Rubber Insulator in Electrical Power Systems
Authors: Meng Xiaobo , Peng Gongmao , Niu Kang , Wang Xiaogang , Mei Hongwei , Wang Liming
Published: June 2022

The migration of low-molecular-weight components of polysiloxane (small molecules) to the surface of high-temperature-vulcanizing silicone rubber (HTV-SR) ensures its hydrophobicity and tends to coat the surface of pollutants, which would otherwise lower hydrophobicity. The transferability of hydrophobicity will ensure the insulator maintains its higher hydrophobicity after being coated with surface pollutants, thus providing the insulator with higher pollution flashover voltage. This migration process takes a certain time, and in this paper, the time characteristics of hydrophobicity transfer from HTV-SR coated with ten different inert materials were investigated. Ten different inert materials have different migration times and static contact angles, possibly due to the influence of pollution material characteristics on the characteristics of small-molecule migration. Thermogravimetric analysis (TG), Fourier transform infrared spectroscopy (FTIR), and gas chromatography–mass spectrometry (GC–MS) were used to analyze the migration of small molecules to the polluted surface. The evidence of small molecules migrating to the surface of the polluted material over time was found. Furthermore, the influence of pollution material characteristics on small-molecule migration was analyzed via SEM, specific surface area, and porosity. On that basis, the hydrophobicity migration characteristics of mixtures of kaolin and kieselguhr were also studied and compared to determine how best to simulate the behavior of natural pollution using artificial pollutants and their mixtures.
Star Polymers vs. Dendrimers: Studies of the Synthesis Based on Computer Simulations
Authors: Polanowski Piotr , Hałagan Krzysztof , Sikorski Andrzej
Published: June 2022

A generic model was developed for studies of the polymerization process of regular branched macromolecules. Monte Carlo simulations were performed employing the Dynamic Lattice Liquid algorithm to study this process. A core-first methodology was used in a living polymerization of stars with up to 32 arms, and dendrimers consisted of 4-functional segments. The kinetics of the synthesis process for stars with different numbers of branches and dendrimers was compared. The size and structure of star-branched polymers and dendrimers during the synthesis were studied. The influence of the functionality of well-defined cores on the structure and on the dispersity of the system was also examined. The differences in the kinetics in the formation of both architectures, as well as changes to their structures, were described and discussed.
Simulation of Binodal and Spinodal Curves of Phase State Diagrams for Binary Polymer Systems
Authors: Chalykh Anatoly E. , Nikulova Uliana V. , Gerasimov Vladimir K.
Published: June 2022

A new approach is proposed for simulating binodal and spinodal curves of phase diagrams for binary polymer systems. It is shown that the Flory–Huggins theory makes it possible to predict phase behavior in a wide range of temperatures and concentrations based on limited data on the components’ solubility. The approbation data of the technique are presented in the example of PS–PB and PS–PMMA systems, for which generalized phase diagrams are constructed.
Textile Strain Sensor Enhancement by Coating Metal Yarns with Carbon-Filled Silicone
Authors: Brendgen Rike , Nolden Ramona , Simon Jasmin , Junge Theresa , Zöll Kerstin , Schwarz-Pfeiffer Anne
Published: June 2022

Flexible and stretchable strain sensors are an important development for measuring various movements and forces and are increasingly used in a wide range of smart textiles. For example, strain sensors can be used to measure the movements of arms, legs or individual joints. Thereby, most strain sensors are capable of detecting large movements with a high sensitivity. Very few are able to measure small movements, i.e., strains of less than 5%, with a high sensitivity, which is necessary to carry out important health measurements, such as breathing, bending, heartbeat, and vibrations. This research deals with the development of strain sensors capable of detecting strain of 1% with a high sensitivity. For this purpose, a total of six commercially available metallic yarns were coated with a carbon-containing silicone coating. The process is based on a vertical dip-coating technology with a self-printed 3D coating bath. Afterwards, the finished yarns were interlooped and stretched by 1% while electrical resistance measurements were carried out. It was shown that, although the coating reduced the overall conductivity of the yarns, it also improved their sensitivity to stress. Conclusively, highly sensitive strain sensors, designed specially for small loads, were produced by a simple coating set-up and interlooping structure of the sensory yarns, which could easily be embedded in greater textile structures for wearable electronics.
Shape Memory Polymer-Based Endovascular Devices: Design Criteria and Future Perspective
Authors: Pineda-Castillo Sergio A. , Stiles Aryn M. , Bohnstedt Bradley N. , Lee Hyowon , Liu Yingtao , Lee Chung-Hao
Published: June 2022

Devices for the endovascular embolization of intracranial aneurysms (ICAs) face limitations related to suboptimal rates of lasting complete occlusion. Incomplete occlusion frequently leads to residual flow within the aneurysm sac, which subsequently causes aneurysm recurrence needing surgical re-operation. An emerging method for improving the rates of complete occlusion both immediately after implant and in the longer run can be the fabrication of patient-specific materials for ICA embolization. Shape memory polymers (SMPs) are materials with great potential for this application, owing to their versatile and tunable shape memory properties that can be tailored to a patient’s aneurysm geometry and flow condition. In this review, we first present the state-of-the-art endovascular devices and their limitations in providing long-term complete occlusion. Then, we present methods for the fabrication of SMPs, the most prominent actuation methods for their shape recovery, and the potential of SMPs as endovascular devices for ICA embolization. Although SMPs are a promising alternative for the patient-specific treatment of ICAs, there are still limitations that need to be addressed for their application as an effective coil-free endovascular therapy.
X-ray Ptychographic Imaging and Spectroscopic Studies of Plasma-Treated Plastic Films
Authors: Ravandeh Mehdi , Mehrjoo Masoud , Kharitonov Konstantin , Schäfer Jan , Quade Antje , Honnorat Bruno , Ruiz-Lopez Mabel , Keitel Barbara , Kreis Svea , Pan Rui , Gang Seung-gi , Wende Kristian , Plönjes Elke
Published: June 2022

Polyethylene terephthalate (PET) is a thermoplastic polyester with numerous applications in industry. However, it requires surface modification on an industrial scale for printing and coating processes and plasma treatment is one of the most commonly used techniques to increase the hydrophilicity of the PET films. Systematic improvement of the surface modification by adaption of the plasma process can be aided by a comprehensive understanding of the surface morphology and chemistry. However, imaging large surface areas (tens of microns) with a resolution that allows understanding the surface quality and modification is challenging. As a proof-of-principle, plasma-treated PET films were used to demonstrate the capabilities of X-ray ptychography, currently under development at the soft X-ray free-electron laser FLASH at DESY, for imaging macroscopic samples. In combination with scanning electron microscopy (SEM), this new technique was used to study the effects of different plasma treatment processes on PET plastic films. The studies on the surface morphology were complemented by investigations of the surface chemistry using X-ray photoelectron spectroscopy (XPS) and Fourier transform infrared spectroscopy (FT-IR). While both imaging techniques consistently showed an increase in roughness and change in morphology of the PET films after plasma treatment, X-ray ptychography can provide additional information on the three-dimensional morphology of the surface. At the same time, the chemical analysis shows an increase in the oxygen content and polarity of the surface without significant damage to the polymer, which is important for printing and coating processes.