bluuebunny/crossref_metadata_2025 huggingface dataset:
Potencialidades de la agencia Gaviota Tours S.A. en la organización de bodas para al mercado interno
Authors: Manso Almanza Rachel , Abreu Rodríguez Daikel Adriano
Published: July 2022

Los eventos han sido imprescindibles en el desarrollo de las civilizaciones, ya sean para celebrar etapas de la vida, períodos estacionales o celebraciones de carácter religioso. Dentro de todos los fenómenos considerados eventos, los eventos planeados son de gran importancia tanto a nivel colectivo como personal, ya que son componentes fundamentales de la cultura, los negocios y la vida cotidiana. Los eventos planeados comprenden dentro de sus clasificaciones a los eventos privados, los cuales son parte del desarrollo de la vida de las personas y se pueden incluir en sus momentos de ocio o esparcimiento. Las bodas son el evento privado más importante para el turismo, las parejas buscan celebrar el matrimonio en lugares especiales y de una forma original y personalizada. En Cuba existe un mercado real en este sentido, las empresas compiten para satisfacer las demandas del segmento nupcial. El objetivo principal de esta investigación fue determinar las potencialidades de la Agencia de Viajes Gaviota Tours S.A en la organización de bodas para el mercado interno. Se trazaron como objetivos específicos; establecer un marco teórico como sustento del desarrollo de la investigación, caracterizar el contexto actual entorno a la organización de bodas y analizar el estado de la modalidad de evento en la empresa. Para cumplir con los objetivos se partió de un análisis bibliográfico que permitió sentar las bases teóricas de la investigación. Posteriormente, se realizó un análisis estratégico dividido en tres etapas fundamentales: Análisis del entorno general, análisis del entorno competitivo y análisis interno de la entidad objeto de estudio. Se utilizaron fuentes primarias y secundarias de información, la toma de datos en el campo se realizó a través de un muestreo no probabilístico por bola de nieve ya que no se conoce el tamaño de la población; el instrumento utilizado fue la entrevista tanto semi-estructurada como no estandarizada y las informaciones obtenidas fueron procesadas estadísticamente. Dentro de los principales resultados se obtuvo que el sector nupcial se encuentra en proceso de perfeccionamiento y crecimiento. A pesar de las deficiencias detectadas en la agencia, la infraestructura que posee, las estructuras organizativas, los productos de los que dispone, los servicios personalizados que ofrece y la experiencia del personal en materia de eventos constituyen potencialidades claras para penetrar en el mercado de la organización de bodas.
ВИКОРИСТАННЯ ТЕХНОЛОГІЙ МЕТОДОЛОГІЇ НАУКИ У ДОСЛІДЖЕННЯХ МОРСЬКОГО ТА ВНУТРІШНЬОГО ВОДНОГО ТРАНСПОРТУ
Authors: Голіков Володимир Антонович , Голіков  , Онищенко Олег Анатолійович
Published: July 2022

На основі ретроспективного аналізу показано, що у прикладних наукових дослідженнях водного транспорту, морської інфраструктури у цілому, високоефективним є використання формальних прийомів у організації таких досліджень. На прикладі побудови технологічних карт і різноманітних структур наукового дослідження доведена ефективність їх використання на практиці. Показано, що структурна візуалізація мети і проміжних завдань є дієвим засобом представлення загального, "оптимального" за витратами часу і ресурсів, плану наукової праці, обґрунтування її актуальності і практичної значущості, достовірність яких перевіряється за допомогою імітаційного та/або натурного експерименту. Формування загальної структури наукового дослідження дозволяє ефективно вичленувати "головні" і "допоміжні" наукові завдання, представляти загальну оцінку і верифікацію отриманих наукових результатів, узагальнювати і узгоджувати результати дослідження і висновки. Показані додаткові можливості використання "технологічних" прийомів для побудови логічного і однозначного формулювання наукової новизни дослідження. Використання загальних принципів технології у методології наукових досліджень будь-якого типу практично перевірено і апробовано на кафедрі технічної експлуатації флоту Національного університету "Одеська морська академія". Подальшим розвитком використання технологій у процесі наукових досліджень має бути уточнення окремих структурних елементів технологічних карт, що забезпечують гарантоване досягнення наукового результату, "астатизм" у кінцевих рішеннях і висновках.
Ключові слова: метод, науковий результат, технологічна карта, морський транспорт, структурна схема, верифікація
LITERATUR REVIEW TENTANG SIKAP DAN PENGETAHUAN PETUGAS MEDIS TERHADAP KELNGKAPAN PENGISIAN RESUME MEDIS RAWAT INAP
Authors: Handayuni Linda , Yenni Ririn Afrima , Mardiawati Dewi , Yenti Melda
Published: July 2022

A medical resume is a summary of all services received by patients during treatment or treatment carried out by health workers. The incompleteness of filling in inpatient medical resumes in hospitals still exists such as lack of attitude and knowledge of health workers, busyness of doctors, absence of SOPs, disagreement with the time limit for filling out medical resumes, which is 1x24 hours. The purpose of this study was to describe the attitudes and knowledge of medical officers to the completeness of filling out an inpatient medical resume. The data used is secondary data, then the data is analyzed by looking for similarities, dissimilarities, views, comparisons and summaries of several research results. The results of the study from 10 journals showed that there were still incomplete medical resumes, due to the lack of knowledge and attitude of officers regarding the importance of completing medical resumes, the unavailability of SOPs for filling out resumes, busy doctors serving patients, resulting in limited time to complete them and the discipline of the doctor in charge. which is still low. From the researcher's analysis, it was concluded that the high number of completeness of completing inpatient medical resumes in hospitals was due to undisciplined officers and the lack of knowledge of officers about filling out resumes. In order to increase the completeness of filling in inpatient medical resumes in hospitals, health workers need to cooperate with each other in filling out medical record documents, especially medical resume sheets in order to create a good, complete and accurate filling.
SANITASI LINGKUNGAN DAN PERILAKU KELUARGA TERHADAP KEJADIAN PENYAKIT DEMAM BERDARAH DENGUE (DBD)
Authors: Cania Arriy Saputra , Dewi Oktavia , Alamsyah Agus
Published: July 2022

Dengue Hemorrhagic Fever (DHF) is an acute febrile disease accompanied by hemorrhagic manifestations with a tendency to cause shock and can cause death, generally attacks children < 15 years, but it is possible to attack adults. In Rokan Hulu there was an increase in dengue cases for three years. the last year starting from 2017-2019. In 2017 there were 61 cases with an incidence rate of 15.95 per 100,000 population, in 2018 there were 77 cases with an incidence rate of 20.13 per 100,000 population, and in 2019 there were 79 cases with an incidence rate of 20.65 per 100,000 population.Objective: to determine the relationship between environmental sanitation and family behavior on the incidence of Dengue Hemorrhagic Fever (DHF) in Ujung Batu District, Rokan Hulu Regency.Methods: This type of research is quantitative and case control design. The population in this study were all households in the Ujung Batu Health Center, with a sample of 105 people, the sampling technique used was simple random sampling. Using primary data, with instruments using observation sheets and questionnaires. Data analysis used univariate, bivariate and multivariate analysis.Results: The type of water reservoir that does not meet the requirements has a risk of 7.183 times the incidence of DHF, the habit of sleeping in the morning and evening has a risk of 2.811 times to experience the incidence of DHF, and the cofounding variable is ventilation.Conclusion: The type of water reservoir that does not meet the requirements and the habit of sleeping in the morning and afternoon have a significant effect on the incidence of dengue fever (DHF).
IMPLEMENTASI PENYALURAN DANA ALOKASI KHUSUS FISIK BIDANG KESEHATAN DALAM MENDUKUNG PEMULIHAN KESEHATAN
Authors: Ramdan Irvanjaya , Malik Syahrizal Suwardana , Purnama Dera Toni
Published: July 2022

The purpose of this study was to determine the implementation, obstacles and efforts made by the Subang District Health Office in the distribution of the physical Special Allocation Fund (DAK) in the healthsector in supporting post-covid-19 health recovery. The theory used by the author is Edward III's theory by analyzing the dimensions of Information Delivery, Resources, Disposition, and Bureaucratic Structure. The research method used is descriptive qualitative research with an inductive approach. The data collection technique used in this research is the technique of Observation, Interview, and Documentation. The results showed that the implementation of Fund Distribution The Special Allocation for Physical Health (DAK) for Physical Health has been running in accordance with the applicable technical guidelines, but in its realization there are still problems such as problems in the development of Labkesda. Obstacles faced such as the lack of trust of the Subang Regency government towards the Subang Regency Health Office which caused the planning program to be delayed, all meetings using the virtual method due to the unfinished Covid-19 condition causing uncertainty, there were still errors in the preparation of letters related to the implementation of the Physical DAK. Health, and the condition of goods that have run out while being procured.
Role of MPV in Early Diagnosis of Vascular Complication in Diabetic Patient
Authors: Khare Manika
Published: July 2022

Diabetes mellitus is a metabolic disease characterized by chronic hyperglycemia resulting from deficiency of insulin. Among platelet indices, Mean Platelet Volume (MPV) demonstrates the changes in rate of platelet production. Increase in MPV is seen in metabolic syndrome, stroke and diabetes. In these 100 diabetics and 100 control subjects were taken and their HbA1c was correlated with the MPV level of these subjects. A significant correlation was found between the MPV and Hba1c levels. Hence raised MPV levels can be used as an early indicator for the complications of diabetes.
Sistem Pendukung Keputusan Diagnosa Gastroenteritis Menggunakan Metode Analytical Hierarchy Process
Authors: Umarsyah Fahmy , Bagaskara Naufal , Jumaryadi Yuwan
Published: July 2022

Penyakit diare masih merupakan masalah kesehatan masyarakat di negara berkembang seperti di Indonesia, berdasarkan survei hasil data dari puskesmas Kembangan selama 2 bulan terakhir mengalami peningkatan. Dimana orang-orang masih mudah terkena penyakit tersebut dikarenakan kurangnya kebersihan serta pola hidup yang kurang sehat. Gastroenteritis merupakan suatu penyakit dimana orang akan merasakan diare serta muntah yang diakibatkan oleh peradangan atau infeksi pada dinding saluran pencernaan, terutama usus dan lambung. Untuk itu kami disini ingin membuat sebuah sistem pendukung keputusan diagnosa Gastroenteritis menggunakan metode Analytical Hierarchy Process (AHP) yang berguna membantu masyarakat dalam mendiagnosa awal penyakit Gastroenteritis. Dalam beberapa penelitian sebelumnya sistem pendukung keputusan tentang Gastroenteritis belum ada. Untuk itu pada penelitian kali ini kami memutuskan menerapkan metode Analytical Hierarchy Process (AHP) karena metode tersebut dinilai lebih akurat dalam mengambil suatu keputusan dalam gejala Gastroenteritis. Hasil dari penelitian ini yaitu berdasarkan hasil perhitungan yang dilakukan bahwa pasien M memiliki nilai dengan indicator tertinggi, dimana pasien tersebut berkemungkinan paling besar terkena Gastroenteritis. Untuk pasien lainnya berkemungkinan kecil terkena Gastroenteritis.
Pengembangan Transformasi Digital Sistem Pemilihan Senat dan Dewan Mahasiswa Menggunakan Metode Research and Developments
Authors: Kamal Muhammad Rikzam
Published: July 2022

Kampus menjadi salah satu unsur terpenting dalam meyakinkan kepada masyarakat berkaitan dengan keamanan data. Oleh karena itu, peneliti melakukan penelitian dan pengembangan e-voting berbasis web dan menerapkannya di IAIN Pekalongan dalam proses pemilihan Senat Mahasiswa (SEMA) dan Dewan Eksekutif Mahasiswa (DEMA) sebagai pilot project yang nantinya bisa diadopsi oleh kampus lain dalam penyelenggaraan pemilu mahasiswa di masa pandemi. Dengan e-voting berbasis web yang dikembangkan dengam menggunakan metode research and development (R&D), mahasiswa IAIN Pekalongan (sebagai pemilih) tidak perlu lagi datang ke kampus untuk menggunakan hak suaranya dalam pemilihan SEMA dan DEMA baik untuk tingkat Institut maupun tingkat fakultas. Hasil dari penelitian menunjukan bahwa e-voting dikembangkan sesuai dengan kondisi di IAIN Pekalongan, bisa menjadi solusi pemilu mahasiswa, dan mudah digunakan sehingga membuat minat pemilih menjadi meningkat.
Woolfian Love in Aggregate: Posthuman – Queer – Feminist
Authors: D. Hagen Benjamin
Published: July 2022

Applying Barbara Herrnstein Smith’s insights into the contingency of value to the contingency of theory’s value, this essay situates Virginia Woolf’s Night and Day (1919) in relation to three critical frameworks. It argues that Woolf’s complication of love in the novel responds to three amorous ‘needs’ articulated, respectively, in the work of Rosi Braidotti, Eve Kosofksy Sedgwick and Sara Ahmed. In bringing Woolf’s novel to the needs voiced by these theorists, the essay neither synthesizes Braidotti, Sedgwick and Ahmed nor privileges one of them above the others. It shows, rather, that Night and Day keeps love unmastered by any single critical paradigm and that its literary/conceptual work is best read in aggregate – read and reread, that is, according to three often antagonistic frameworks between which the essay makes a temporary peace. The posthuman and queer frames bring to life Katharine Hilbery’s powerful attachment to and preference for the study of mathematics and astronomy, her distaste for human beings (and literary studies), and her careful stagecraft in managing the people she loves. The feminist frame links the promises of love to the patriarchal/misogynistic division between the education of men and the training of women.
Wolves Like to Wander Around: Nomadic, Distal, and Unfurling Forces in Maclear and Arsenault's Virginia Wolf
Authors: Rohman Carrie
Published: July 2022

Perhaps where it is least expected in the literary arts – within a book for children about Virginia Woolf and her sister – the linguistic and visual narrative highlights a therapeutic, bioaesthetic power that is fundamentally nonhuman and trans-species. Virginia and Vanessa, the ‘Bloomsberry’ sisters in this text, undertake a shared creative practice and enlivenment that is creatural, intimate, wolfy, and filled with eco-aesthetic intensities. The story’s theme of a re-vitalizing capacity to become artistic highlights the nomadic and distalic, and the sisters engage with shared cosmic, earth, and animal shapes, sounds, colours and forces. The recuperative efforts imagined for these famous modernist sisters dwell in female relationality, but the text also acknowledges the vulnerability of living ‘on the cracks of life’, an acknowledgment Braidotti insists must remain part of the healthy life that recognizes and connects to pain, even as it works toward an affirmative ethics of biopower.
Somatechnics, Styles of Reasoning, and the Making of the Subject of Suicidology
Authors: Tack Saartje
Published: July 2022

Suicidology calls itself and is recognised as the science of suicide and suicide prevention. Findings from suicidological research are widely acknowledged as truth and inform some of our most profound ways of knowing and experiencing suicide today in the west: suicide is the result of mental illness, it is located in the interiority of the individual, and it is a problem that must be prevented. Suicidologists make these claims by using quantitative research methods, which they propose allow them to study suicide objectively and to find its truth. This paper investigates the somatechnics of research at play in suicidology that allow the field to maintain its position of authority in both academic and public discussions of suicide. It thus analyses how suicide has come to be known in suicidology, and how these knowledges are sustained as the truth of suicide. In bringing Hacking’s concept of ‘styles of reasoning’ to bear on suicidology, the paper identifies the ways in which suicidology puts in place narrow parameters for what can be known about suicide and how it can be known. In doing so, the paper argues that what has come to constitute neutral, evidence-based knowledge about suicide both in this field of research and in public understandings of suicide is the result of an elaborate somatechnic machinery aimed at the regulation of knowledge and at the preservation of an image of scientificity and the position of authority of the field, rather than being the truth of suicide. In doing so, the paper argues that the suicidological style of reasoning is a somatechnic that is implicated in in/trans/forming both the body of the suicidal person and that of the expert suicide researcher as particular kinds of subjects in mutually generative ways.
WORKSHOP PARA EDUCADORES PRÉ-ESCOLARES E FUNDAMENTAL I SOBRE NEURODESENVOLVIMENTO INFANTIL E CRIANÇAS COM DIFICULDADES DE LEITURA E ESCRITA
Authors:   Universidade Presbiteriana MACKENZIE, PEDROSO Yara de Castro Ganme
Published: July 2022

Scientific evidence shows that children diagnosed and treated at the beginning of the manifestations of difficulties even in learning may have the effects of this problematic difficulty in education, not being more noticeable in the course of their schooling. For children not to show vision, not diagnosed and treated, the opposite is true, and serious schooling can last or evolve. The expected result, after the training carried out via the workshop, is an increase in the disinformation of educators on the subject, as well as promoting an increase in readiness for the possibility of problems related to children with learning difficulties. Justifies carrying out the intervention, the idea that children often present their school learning and, if they are not treated, they may have probabilities that, many times, they can present learning. The work is still essential, due to the fact that there are flaws in the teaching curricula of professionals who work with literacy, causing the intervention to overcome the teachers' lack of knowledge about neurodevelopment, as well as the early care of children, the which invariably occurs only when the damage has already occurred. The objective of the work, in short, is to propose a model of conduct and early diagnosis for the detection of learning problems that have become chronic in schools. Methodologically, this intervention will take place through workshops on child neurodevelopment, capacity building and teacher training for the perception of educators in relation to their students, so that they can detect early warning signs about possible school deficits in the Preschool phases. School and Elementary I, resulting or not from some cognitive problem.
Electricity Demand Pattern and Supply Availability On Nigeria Grid System
Authors: Ale Thomas , Adeyemi Adeleye
Published: July 2022

This paper presents the analysis of electricity transmitted and demand on Nigeria's electricity grid system from the year 2018 to 2020 to give the present progress of the electricity system in Nigeria. The daily electricity generated and transmitted data, daily distribution companies (DISCOs) electricity demand and consumption data, and data of transmission lines connected to other neighbouring countries (international lines) within the year 2018 and 2020 were used for the analysis. Also, the extrapolation of the monthly energy of each of the data obtained was computed. The analysis was done and graphs and results obtained showed that daily average electricity day-ahead demand by DISCOs varied majorly between 3.5GW to 4GW with a corresponding increase above 4GW and the total daily day-ahead electricity demand by DISCOs varied majorly between 80GW and 90GW from 2018 to 2020. But despite this demand, the study showed that distribution companies did not at any time pick up to their declared load demand despite being the major electricity stakeholder in electricity delivery to consumers. Also, some generating station units were not generating to their capacity due to fault and gas constraints and some generating stations were connected to the grid without using free governor mode (control required for the generating units to respond to the state of electricity demand on the grid in real-time). The study recommends that the government should ensure proper monitoring and impose necessary sanctions if needs be on any electricity stakeholders and participants who violate the Nigeria Electricity Supply Market Rules for effective and the Nigeria grid code created for efficient power delivery. The government should, as a matter of urgency, start the expansion of her generating stations as well as developing new ones considering other sources for power generation such as wind and solar which are predominately abundant in the northern part of the country.
Viscosities, Free Energies of Activation and their Excess Properties in the Binary Mixtures of Some Monoalkanolamines with Acetonitrile between 303.15 and 323.15 K: Experimental and Correlative Approach
Authors: Muhammad A. R. Khan  , M. Mehedi Hasan Rocky  , Md. Ariful Islam  , I Chowdhury Faisal , M. Shamsuddin Ahmed  , Shamim Akhtar
Published: July 2022

Viscosities (h) of three binary non-aqueous systems of ACN + MEA, + MMEA and + MEEA have been measured in the whole range of compositions at temperatures ranging between 303.15 and 323.15 K at an interval of 5 K. At different compositions, deviations in viscosity (Dh), free energy (ΔG‡) of activation for viscous flow along its excess values (ΔG‡E) were calculated from experimental ρ andh data. For all systems, h vs. x2 initially changed very slowly, but with the increment of solute concentration h were found to rise quite rapidly. The values of Dh were largely positive and they formed a sharp maximum invariably at the highly alkanolamine-rich regions. All positive values of Dh followed the increasing order as: ACN + MMEA > ACN + MEA > ACN + MEEA. The order of DG‡E at the maximum point was ACN + MMEA > ACN + MEA > ACN + MEEA. For the correlative model, zero parameter relations: Bingham, Kendall- Munroe, Gambill, and Eyring relations, one parameter relations: Hind, Grunberg-Nissan, Frenkel, Wijk, Katti-Chaudhri, Tamura Kurata and two as well as three parameter-based models: Heric, Ausländer, McAllister (3-body) and McAllister (4-body) Equation and the Jouyban-Acree model (JA) were employed to correlate viscosities. Ausländer equation fit the best for: ACN + MEA.  McAlliester 4-body fit the best for ACN + MMEA and ACN + MEEA. All the above results were attempted to be interpreted in terms of the strength and order of self-association, intra- as well as intermolecular hydrogen bonding via OH···O or OH···N and the effect due to steric hindrance of the concerned alkanolamine molecules and interstitial accommodation of ACN into alkanolamine network.
Peningkatan Ketrampilan Merancang Identitas Visual Pada Produk Umkm Binaan Serabut Nusa Berdaya Kabupaten Semarang-Ungaran
Authors: Vydia Vensy , Putri Astrid Novita , Suntoro Joko
Published: July 2022

Identitas visual yang baik dan kuat merupakan alat di dalam membangun merek karena berfungsi membentuk kesan yang mempengaruhi persepsi konsumen. Semakin lama masyarakat mengenal baik merek tertentu, semakin besar pula kemungkinan mereka bersikap positif terhadap merek itu. Bahkan, dengan mengubah identitas visual sebuah produk, dapat juga mengubah atau memperbaiki citra merek di masyarakat serta menjadikan Identitas. Inilah yang dimaksud dengan konsepsi loyalitas merek, demikian juga sebaliknya apabila konsumen mendapatkan stimulus negatif dari sebuah identitas visual dari sebuah merek maka kemungkinan terbesar adalah konsumen membatalkan keinginan untuk mencoba atau melakukan pembelian berulang. Untuk merepresentasikan sebuah identitas maka dibutuhkan sebuah logo dan sistem visual, sehingga merupakan aset penting dalam sebuah perusahaan, lembaga, maupun produk sebagai identitas. Kebutuhan tersebut sangat diperlukan oleh berbagai lapisan usaha tidak terkecuali UMKM. Program pengabdian masyarakat ini ditekankan pada peningkatan kompetensi pelaku UMKM dalam memaksimalkan potensi jual produk usaha dengan penguatan identitas visual pada logo. Pelaksanaan abdimas ini melibatkan tenaga pengajar (dosen) dari Program Studi Sistem Informasi, Teknik Informatika dan Ilmu Komunikasi serta mahasiswa. Program ini menggandeng sebanyak 15 (Limabelas) peserta UMKM untuk dibuat desain identitas serta media aplikasinya.. Dalam pelaksanaannya, perancangan dimulai dari analisis produk untuk kemudian dibuat output visual, dari mulai ilustrasi sampai warna.
Keywords: Desain, Logo, Identitas, UMKM, Visual
Peningkatan Produktivitas di Era Pandemi Covid 19 dengan Pengolahan Sampah Menjadi Eco Enzyme
Authors: Putri Noventia Karina , Ira Setiawati
Published: July 2022

Pengolahan sampah organik dapat menjadi program pemberdayaan masyarakat khususnya bagi para ibu rumah tangga. Program ini bertujuan untuk memberi wawasan kepada ibu rumah tangga bahwa sampah juga dapat diolah menjadi sesuatu yang lebih bermanfaat dan bernilai ekonomis yang tinggi. Tujuan dari pengabdian adalah meningkatkan produktivitas para ibu rumah tangga di RW 05 Kelurahan Kembangarum, Kecamatan Mranggen, Kabupaten Demak. Pelaksanaan kegiatan pengolahan sampah organik menjadi eco enzyme berjalan sangat baik. Sampah organik yang dipakai dalam pengolahan ini adalah sampah sayur mayur, sampah kulit buah-buahan serta buah yang sudah membusuk. Metode yang dilakukan dalam pengabdian ini adalah presentasi atau ceramah dan demonstrasi.Keyword: Sampah Organik, Eco Enzyme, Pengabdian
ELIZABETH BISHOP’S ONE ART: PHONOLOGICAL ANALYSIS
Authors:   Madridejos Community College, Madridejos Cebu., G. GIDO NATHANIEL , S. CASIÑO MICHAEL VINCENT ,   Tagoloan Community College, Baluarte, Tagoloan, Misamis Oriental, HALINA KISHA L. ,   Tagoloan Community College, Baluarte, Tagoloan, Misamis Oriental
Published: July 2022

Elizabeth Bishop Poem's One Art is utilizing textual analysis employing phonological analysis. The poem reveals rhyming sounds that are evident in poem like alliteration and assonance. It is argued that Bishop's poetry achieves real power just by being honest. In order to connect with her audience and write a poem about a subject that many people, especially women, care about, the poet, a woman draws on her own life experiences. She touches on a sensitive subject for many women when she mentions losing a gold watch or forgetting names: the dread of losing something dear to them, like their mother's watch, or of becoming older and forgetting things or experiencing memory issues. It's important to keep in mind that the poet's own experiences and viewpoints influence the poetry and aid in making the audience believe it. The reader will see a comparison of losses between several items in this poetry. The poet employed rich structural and sardonic structure to create a very straightforward but deep poem. She rejects the statement "Loss is not difficult to maser," so you can tell. The final line's shift to "Loss is not so difficult to learn" may be a sign that the poet is finding it harder and harder to cope with the loss of his loved one. Bishop demonstrates how embracing loss and losing the little things enhances the ironic nature of poetry. She is, after all, a tiny "one art." She thinks she can start a new life and progress if she can get out of pain and let go of her emotions, but she finds that to be too challenging. Bishop turns losing into an art form and investigates how, if we can master it, we may become detached from the hurt of loss. Elizabeth Bishop's father passed away when she was just eight months old, her mother later died of a mental condition, and she eventually lost her lover to suicide. We could therefore consider this poem to be partially autobiographical. In it, the poet lists a number of things we might lose in life in order of increasing importance, with the loss of a loved one serving as the list's ultimate resolution.
Language as a Resource of Identity in Europe: The Anthropological Study
Authors:   Miklouho-Maclay the Russian Academy of Sciences Institute of Ethnology and Anthropology, Martynova Marina
Published: July 2022

The collective monograph publishes the results of research carried out in accordance with the theme of the scientific work "Culturally Complex Societies: Analysis of World Experience". The monitoring of the linguistic situation, ethnic and cultural diversity of the European states was carried out by the staffs of the Center for European Studies of the Miklouho-Maclay the Russian Academy of Sciences Institute of Ethnology and Anthropology and the Department of European Studies of the Peter the Great Museum of Anthropology and Ethnography (Kunstkamera). The researchers did not set themselves the task of studying linguistic problems, but attempted to consider the social aspect of the language functioning. The book should be usefull for specialists in the field of interethnic relations, linguistic, educational and migration policy, scholars of the humanities and representatives of public organizations.
Bacteriophage Genetic Edition Using LSTM
Authors: Ataee Shabnam , Brochet Xavier , Peña-Reyes Carlos Andrés
Published: July 2022

Bacteriophages are gaining increasing interest as antimicrobial tools, largely due to the emergence of multi-antibiotic–resistant bacteria. Although their huge diversity and virulence make them particularly attractive for targeting a wide range of bacterial pathogens, it is difficult to select suitable phages due to their high specificity which limits their host range. In addition, other challenges remain such as structural fragility under certain environmental conditions, immunogenicity of phage therapy, or development of bacterial resistance. The use of genetically engineered phages may reduce characteristics that hinder prophylactic and therapeutic applications of phages. Nowadays, there is no systematic method to modify a given phage genome conferring its sought characteristics. We explore the use of artificial intelligence for this purpose as it has the potential to both guide and accelerate genome modification to generate phage variants with unique properties that overcome the limitations of natural phages. We propose an original architecture composed of two deep learning–driven components: a phage–bacterium interaction predictor and a phage genome-sequence generator. The former is a multi-branch 1-D convolutional neural network (1D-CNN) that analyses phage and bacterial genomes to predict interactions. The latter is a recurrent neural network, more particularly a long short-term memory (LSTM), that performs genomic modifications to a phage to offer substantial host range improvement. For this component, we developed two different architectures composed of one or two stacked LSTM layers with 256 neurons each. These generators are used to modify, more precisely to rewrite, the genome sequence of 42 selected phages, while the predictor is used to estimate the host range of the modified bacteriophages across 46 strains of Pseudomonas aeruginosa. The proposed generators, trained with an average accuracy of 96.1%, are able to improve the host range for an average of 18 phages among the 42 under study, increasing both their average host range, by 73.0 and 103.7%, and the maximum host ranges from 21 to 24 and 29, respectively. These promising results showed that the use of deep learning methodologies allows genetic modification of phages to extend, for instance, their host range, confirming the potential of these approaches to guide bacteriophage engineering.
MLP-Based Regression Prediction Model For Compound Bioactivity
Authors: Qin Yongfei , Li Chao , Shi Xia , Wang Weigang
Published: July 2022

The development of breast cancer is closely linked to the estrogen receptor ERα, which is also considered to be an important target for the treatment of breast cancer. Therefore, compounds that can antagonize ERα activity may be drug candidates for the treatment of breast cancer. In drug development, to save manpower and resources, potential active compounds are often screened by establishing compound activity prediction model. For the 1974 compounds collected, the top 20 molecular descriptors that significantly affected the biological activity were screened using LASSO regression models combined with 10-fold cross-validation method. Further, a regression prediction model based on the MLP fully connected neural network was constructed to predict the bioactivity values of 50 new compounds. To measure the validity of the model, the model loss term was specified as the mean squared error (MSE). The results showed that the MLP-based regression prediction model had a loss value of 0.0146 on the validation set. This model is therefore well trained and the prediction strategy used is valid. The methods developed by this paper may provide a reference for the development of anti-breast cancer drugs.
Cathepsin K+ Non-Osteoclast Cells in the Skeletal System: Function, Models, Identity, and Therapeutic Implications
Authors: Zou Nanyu , Liu Ran , Li Changjun
Published: July 2022

Cathepsin K (Ctsk) is a cysteine protease of the papain superfamily initially identified in differentiated osteoclasts; it plays a critical role in degrading the bone matrix. However, subsequent in vivo and in vitro studies based on animal models elucidate novel subpopulations of Ctsk-expressing cells, which display markers and properties of mesenchymal stem/progenitor cells. This review introduces the function, identity, and role of Ctsk+ cells and their therapeutic implications in related preclinical osseous disorder models. It also summarizes the available in vivo models for studying Ctsk+ cells and their progeny. Further investigations of detailed properties and mechanisms of Ctsk+ cells in transgenic models are required to guide potential therapeutic targets in multiple diseases in the future.
Disease-Associated Regulation of Non-Coding RNAs by Resveratrol: Molecular Insights and Therapeutic Applications
Authors: Giordo Roberta , Wehbe Zena , Posadino Anna Maria , Erre Gian Luca , Eid Ali H. , Mangoni Arduino A. , Pintus Gianfranco
Published: July 2022

There have been significant advances, particularly over the last 20 years, in the identification of non-coding RNAs (ncRNAs) and their pathophysiological role in a wide range of disease states, particularly cancer and other chronic conditions characterized by excess inflammation and oxidative stress such as atherosclerosis, diabetes, obesity, multiple sclerosis, osteoporosis, liver and lung fibrosis. Such discoveries have potential therapeutic implications as a better understanding of the molecular mechanisms underpinning the effects of ncRNAs on critical homeostatic control mechanisms and biochemical pathways might lead to the identification of novel druggable targets. In this context, increasing evidence suggests that several natural compounds can target ncRNAs at different levels and, consequently, influence processes involved in the onset and progression of disease states. The natural phenol resveratrol has been extensively studied for therapeutic purposes in view of its established anti-inflammatory and antioxidant effects, particularly in disease states such as cancer and cardiovascular disease that are associated with human aging. However, increasing in vitro and in vivo evidence also suggests that resveratrol can directly target various ncRNAs and that this mediates, at least in part, its potential therapeutic effects. This review critically appraises the available evidence regarding the resveratrol-mediated modulation of different ncRNAs in a wide range of disease states characterized by a pro-inflammatory state and oxidative stress, the potential therapeutic applications, and future research directions.
Identification of an m6A-Related Long Noncoding RNA Risk Model for Predicting Prognosis and Directing Treatments in Patients With Colon Adenocarcinoma
Authors: Liao Wanying , Long Junyu , Li Yiran , Xie Fucun , Xun Ziyu , Wang Yanyu , Yang Xu , Wang Yunchao , Zhou Kang , Sang Xinting , Zhao Haitao
Published: July 2022

N6-methyladenosine (m6A) and lncRNAs have been implicated in the development of colon cancer, including tumorigenesis, migration, and invasion. However, the specific effect of m6A regulators on lncRNAs is not clear, and m6A-related lncRNAs may be new prognostic biomarkers and may help direct treatment and medication. We identified 29 prognostic m6A-related lncRNAs and constructed a risk model using 12 lncRNAs. The model was an independent prognostic factor and could accurately predict the prognosis. A stable and robust nomogram that combined the model and pathologic stage was constructed. A total of 2,424 differentially expressed genes (DEGs) were identified based on the model. Functional analysis of the DEGs showed that they were associated with tumor progression, helping investigate the underlying biological functions and signaling pathways of the risk model. In addition, the low-risk group based on the risk model had more sensitivity to afatinib, metformin, and GW.441756, and patients with low risk would more likely respond to immunotherapy. Moreover, patients with higher risk were more sensitive to olaparib, bexarotene, and doxorubicin.
A Novel Role of PP2A Methylation in the Regulation of Tight Junction Assembly and Integrity
Authors: Schuhmacher Diana , Sontag Jean-Marie , Sontag Estelle
Published: July 2022

Tight junctions (TJs) are multiprotein complexes essential for cell polarity and the barrier function of epithelia. The major signaling molecule, protein serine/threonine phosphatase 2A (PP2A), interacts with the TJ and modulates the phosphorylation state of TJ proteins. An important PP2A regulatory mechanism involves leucine carboxyl methyltransferase-1 (LCMT1)-dependent methylation and protein phosphatase methylesterase-1 (PME1)-mediated demethylation of its catalytic subunit on Leu309. Here, using MDCK cells, we show that overexpression of LCMT1, which enhances cellular PP2A methylation, inhibits TJ formation, induces TJ ruffling, and decreases TJ barrier function. Conversely, overexpression of PME1 accelerates TJ assembly and enhances TJ barrier function. PME1-dependent PP2A demethylation increases during early Ca2+-dependent junctional assembly. Inhibition of endogenous PME1 delays the initial Ca2+-mediated redistribution of TJ proteins to cell-cell contacts and affects TJ morphology and barrier function. Manipulating one-carbon metabolism modulates TJ assembly, at least in part by affecting PP2A methylation state. The integrity of PP2A methylation is critical for proper targeting of PP2A to the TJ. It is necessary for PP2A complex formation with the TJ proteins, occludin and ZO-1, and proteins of the PAR complex, Par3 and atypical protein kinase C ζ (aPKCζ), which play a key role in development of cell polarity. Expression of a methylation incompetent PP2A mutant induces defects in TJ assembly and barrier function. aPKCζ-mediated Par3 phosphorylation is also required for targeting of the PP2A ABαC holoenzyme to the TJ. Our findings provide the first evidence for a role of LCMT1, PME1 and PP2A methylation/demethylation processes in modulating TJ assembly and functional integrity. They also position PP2A at the interface of one-carbon metabolism and the regulation of key TJ and polarity proteins that become deregulated in many human diseases.
Targeting of the Lipid Metabolism Impairs Resistance to BRAF Kinase Inhibitor in Melanoma
Authors: Vergani Elisabetta , Beretta Giovanni L. , Aloisi Mariachiara , Costantino Matteo , Corno Cristina , Frigerio Simona , Tinelli Stella , Dugo Matteo , Accattatis Felice Maria , Granata Agnese , Arnaboldi Lorenzo , Rodolfo Monica , Perego Paola , Gatti Laura
Published: July 2022

Drug resistance limits the achievement of persistent cures for the treatment of melanoma, in spite of the efficacy of the available drugs. The aim of the present study was to explore the involvement of lipid metabolism in melanoma resistance and assess the effects of its targeting in cellular models of melanoma with acquired resistance to the BRAF-inhibitor PLX4032/Vemurafenib. Since transcriptional profiles pointed to decreased cholesterol and fatty acids synthesis in resistant cells as compared to their parental counterparts, we examined lipid composition profiles of resistant cells, studied cell growth dependence on extracellular lipids, assessed the modulation of enzymes controlling the main nodes in lipid biosynthesis, and evaluated the effects of targeting Acetyl-CoA Acetyltransferase 2 (ACAT2), the first enzyme in the cholesterol synthesis pathway, and Acyl-CoA Cholesterol Acyl Transferase (ACAT/SOAT), which catalyzes the intracellular esterification of cholesterol and the formation of cholesteryl esters. We found a different lipid composition in the resistant cells, which displayed reduced saturated fatty acids (SFA), increased monounsaturated (MUFA) and polyunsaturated (PUFA), and reduced cholesteryl esters (CE) and triglycerides (TG), along with modulated expression of enzymes regulating biosynthetic nodes of the lipid metabolism. The effect of tackling lipid metabolism pathways in resistant cells was evidenced by lipid starvation, which reduced cell growth, increased sensitivity to the BRAF-inhibitor PLX4032, and induced the expression of enzymes involved in fatty acid and cholesterol metabolism. Molecular targeting of ACAT2 or pharmacological inhibition of SOAT by avasimibe showed antiproliferative effects in melanoma cell lines and a synergistic drug interaction with PLX4032, an effect associated to increased ferroptosis. Overall, our findings reveal that lipid metabolism affects melanoma sensitivity to BRAF inhibitors and that extracellular lipid availability may influence tumor cell response to treatment, a relevant finding in the frame of personalized therapy. In addition, our results indicate new candidate targets for drug combination treatments.
Taking the Path Into the Dark Woods – Initial Industry Impact of the COVID-19 Pandemic
Authors: Thornberg Adam
Published: July 2022

During the initial onset of the COVID-19 pandemic, each industry experienced hardships. One area that has not been explored in great detail was how the diagnostic industry managed while bringing SARS-CoV-2 tests to the market. This perspective piece provides a sample view of what went on behind the walls of a diagnostic manufacturer that released one of the initial SARS-CoV-2 testing options, some of the barriers that were encountered, and how they could be overcome.
The Innate Immune Protein Calprotectin Interacts With and Encases Biofilm Communities of Pseudomonas aeruginosa and Staphylococcus aureus
Authors: Baishya Jiwasmika , Everett Jake A. , Chazin Walter J. , Rumbaugh Kendra P. , Wakeman Catherine A.
Published: July 2022

Calprotectin is a transition metal chelating protein of the innate immune response known to exert nutritional immunity upon microbial infection. It is abundantly released during inflammation and is therefore found at sites occupied by pathogens such as Pseudomonas aeruginosa and Staphylococcus aureus. The metal limitation induced by this protein has previously been shown to mediate P. aeruginosa and S. aureus co-culture. In addition to the transition metal sequestration role of calprotectin, it has also been shown to have metal-independent antimicrobial activity via direct cell contact. Therefore, we sought to assess the impact of this protein on the biofilm architecture of P. aeruginosa and S. aureus in monomicrobial and polymicrobial culture. The experiments described in this report reveal novel aspects of calprotectin’s interaction with biofilm communities of P. aeruginosa and S. aureus discovered using scanning electron microscopy and confocal laser scanning microscopy. Our results indicate that calprotectin can interact with microbial cells by stimulating encapsulation in mesh-like structures. This physical interaction leads to compositional changes in the biofilm extracellular polymeric substance (EPS) in both P. aeruginosa and S. aureus.
Effects of the Sex Steroid Hormone Estradiol on Biofilm Growth of Cystic Fibrosis Pseudomonas aeruginosa Isolates
Authors: Al-Zawity Jiwar , Afzal Faria , Awan Aysha , Nordhoff Daniela , Kleimann Alexander , Wesner Daniel , Montier Tristan , Le Gall Tony , Müller Mareike
Published: July 2022

Women with cystic fibrosis (CF) have a significantly lower life expectancy compared to men, which is indicated by an earlier impairment of lung function due to chronic colonization with biofilm formed by Pseudomonas aeruginosa. There is growing evidence that blood serum concentrations of the steroid sex hormone estradiol (E2) correlate with the occurrence of pulmonary exacerbations in CF but also play a role in the mucoid switch of P. aeruginosa. This study aims to shed light on possible microbiological reasons for sexual dimorphism in CF by investigating the influence of E2 on biofilm formation of P. aeruginosa CF isolates. For this purpose, 10 CF isolates of the respiratory tract derived from different CF patients have been treated with E2 in a microtiter plate biofilm model. Biofilms have been examined by crystal violet assays, field emission scanning electron microscopy (FE-SEM), 3D laser scanning microscopy (LSM), and quorum sensing (QS) reporter assays of the supernatants taken from biofilms. This allowed us to simultaneously investigate the effects of E2 on attached biofilm mass, biofilm ultrastructure, and QS activity. Upon E2 treatment, six out of 10 investigated CF isolates showed an increase of attached biofilm mass, whereas biofilms from two tested non-CF laboratory strains (PAO1 and ATCC19660) did not. Moreover, FE-SEM and 3D LSM analyses of the E2 responsive CF biofilms revealed ultrastructural remodeling of biofilm structure at different scales with increased formation of prominent biofilm spots, enhanced coverage with extracellular polymeric substance (EPS), and extended average surface roughness. QS activity measurements performed in biofilm supernatants via luminescence acyl homoserine lactone (AHL) reporter assays further showed that E2 treatment may also modulate QS signaling, as shown in an E2 sensitive CF isolate. Together, our results suggest the biofilm modulating effects of E2 on various clinical CF isolates that are documented by both biomass and ultrastructural changes of biofilms. The gained new insight into the influence of steroid hormones on P. aeruginosa biofilm phenotypes might pave the way for novel future approaches in personalized medicine based on the patients’ sex and hormonal status.