bluuebunny/crossref_metadata_2025 huggingface dataset:
Physiological and Agronomic Strategies to Increase Mungbean Yield in Climatically Variable Environments of Northern Australia
Authors: Chauhan Yashvir S. , Williams Rex
Published: May 2018

Mungbean (Vigna radiata (L.) Wilczek) in Australia has been transformed from a niche opportunistic crop into a major summer cropping option for dryland growers in the summer-dominant rainfall regions of Queensland and New South Wales. This transformation followed stepwise genetic improvements in both grain yields and disease resistance. For example, more recent cultivars such as ‘Crystal’, ‘Satin II’, and ‘Jade-AU‘ have provided up to a 20% yield advantage over initial introductions. Improved agronomic management to enable mechanised management and cultivation in narrow (<50 cm) rows has further promised to increase yields. Nevertheless, average yields achieved by growers for their mungbean crops remain less than 1 t/ha, and are much more variable than other broad acre crops. Further increases in yield and crop resilience in mungbean are vital. In this review, opportunities to improve mungbean productivity have been analysed at four key levels including phenology, leaf area development, dry matter accumulation, and its partitioning into grain yield. Improving the prediction of phenology in mungbean may provide further scope for genetic improvements that better match crop duration to the characteristics of target environments. There is also scope to improve grain yields by increasing dry matter production through the development of more efficient leaf canopies. This may introduce additional production risks as dry matter production depends on the amount of available water, which varies considerably within and across growing regions in Australia. Improving crop yields by exploiting G × E × M interactions related to cultivar photo-thermal sensitivities and make better use of available water in these variable environments is likely to be a less risky strategy. Improved characterisation of growing environments using modelling approaches could also better define and identify the risks of major abiotic constraints. This would assist in optimising breeding and management strategies to increase grain yield and crop resilience in mungbean for the benefit of growers and the industry.
Neuromodulatory Treatments for Alcohol Use Disorder: A Review
Authors: Salib Anne-Mary N. , Ho Allen L. , Sussman Eric S. , Pendharkar Arjun V. , Halpern Casey H.
Published: May 2018

Alcohol use disorder (AUD) is a prevalent condition characterized by chronic alcohol-seeking behaviors and has become a significant economic burden with global ramifications on public health. While numerous treatment options are available for AUD, many are unable to sustain long-term sobriety. The nucleus accumbens (NAcc) upholds an integral role in mediating reward behavior and has been implicated as a potential target for deep brain stimulation (DBS) in the context of AUD. DBS is empirically thought to disrupt pathological neuronal synchrony, a hallmark of binge behavior. Pre-clinical animal models and pilot human clinical studies utilizing DBS for the treatment of AUD have shown promise for reducing alcohol-related cravings and prolonging abstinence. In this review, we outline the various interventions available for AUD, and the translational potential DBS has to modulate functionality of the NAcc as a treatment for AUD.
CRISPR-Mediated Reactivation of DKK3 Expression Attenuates TGF-β Signaling in Prostate Cancer
Authors: Kardooni Hoda , Gonzalez-Gualda Estela , Stylianakis Emmanouil , Saffaran Sina , Waxman Jonathan , Kypta Robert M.
Published: May 2018

The DKK3 gene encodes a secreted protein, Dkk-3, that inhibits prostate tumor growth and metastasis. DKK3 is downregulated by promoter methylation in many types of cancer, including prostate cancer. Gene silencing studies have shown that Dkk-3 maintains normal prostate epithelial cell homeostasis by limiting TGF-β/Smad signaling. While ectopic expression of Dkk-3 leads to prostate cancer cell apoptosis, it is unclear if Dkk-3 has a physiological role in cancer cells. Here, we show that treatment of PC3 prostate cancer cells with the DNA methyltransferase (DNMT) inhibitor decitabine demethylates the DKK3 promoter, induces DKK3 expression, and inhibits TGF-β/Smad-dependent transcriptional activity. Direct induction of DKK3 expression using CRISPR-dCas9-VPR also inhibited TGF-β/Smad-dependent transcription and attenuated PC3 cell migration and proliferation. These effects were not observed in C4-2B cells, which do not respond to TGF-β. TGF-β signals can regulate gene expression directly via SMAD proteins and indirectly by increasing DNMT expression, leading to promoter methylation. Analysis of genes downregulated by promoter methylation and predicted to be regulated by TGF-β found that DKK3 induction increased expression of PTGS2, which encodes cyclooxygenase-2. Together, these observations provide support for using CRISPR-mediated induction of DKK3 as a potential therapeutic approach for prostate cancer and highlight complexities in Dkk-3 regulation of TGF-β signaling.
Molecular Orientations Change Reaction Kinetics and Mechanism: A Review on Catalytic Alcohol Oxidation in Gas Phase and Liquid Phase on Size-Controlled Pt Nanoparticles
Authors: Liu Fudong , Wang Hailiang , Sapi Andras , Tatsumi Hironori , Zherebetskyy Danylo , Han Hui-Ling , Carl Lindsay M. , Somorjai Gabor A.
Published: May 2018

Catalytic oxidation of alcohols is an essential process for energy conversion, production of fine chemicals and pharmaceutical intermediates. Although it has been broadly utilized in industry, the basic understanding for catalytic alcohol oxidations at a molecular level, especially under both gas and liquid phases, is still lacking. In this paper, we systematically summarized our work on catalytic alcohol oxidation over size-controlled Pt nanoparticles. The studied alcohols included methanol, ethanol, 1-propanol, 2-propanol, and 2-butanol. The turnover rates of different alcohols on Pt nanoparticles and also the apparent activation energy in gas and liquid phase reactions were compared. The Pt nanoparticle size dependence of reaction rates and product selectivity was also carefully examined. Water showed very distinct effects for gas and liquid phase alcohol oxidations, either as an inhibitor or as a promoter depending on alcohol type and reaction phase. A deep understanding of different alcohol molecular orientations on Pt surface in gas and liquid phase reactions was established using sum-frequency generation spectroscopy analysis for in situ alcohol oxidations, as well as density functional theory calculation. This approach can not only explain the entirely different behaviors of alcohol oxidations in gas and liquid phases, but can also provide guidance for future catalyst/process design.
Photocatalytic Behavior of Strontium Aluminates Co-Doped with Europium and Dysprosium Synthesized by Hydrothermal Reaction in Degradation of Methylene Blue
Authors: Park Byung-Geon
Published: May 2018

Strontium aluminates co-doped with europium and dysprosium were prepared by a hydrothermal reaction through a sintering process at lower temperatures. The physicochemical properties of the strontium aluminates co-doped with europium and dysprosium were characterized and compared with those of strontium aluminates prepared by a sol–gel method. The photocatalytic properties of the strontium aluminates co-doped with europium and dysprosium were evaluated through the photocatalytic decomposition of methylene blue dye. The strontium aluminates co-doped with europium and dysprosium prepared by the hydrothermal reaction exhibited good phosphorescence and photocatalytic activities that were similar to those prepared by the sol–gel method. The photocatalytic activity of these catalysts for methylene blue degradation was higher than that of the titanium dioxide (TiO2) photocatalyst.
On the Impact of the Preparation Method on the Surface Basicity of Mg–Zr Mixed Oxide Catalysts for Tributyrin Transesterification
Authors: Rabee Abdallah I. M. , Manayil Jinesh C. , Isaacs Mark A. , Parlett Christopher M. A. , Durndell Lee J. , Zaki Mohamed I. , Lee Adam F. , Wilson Karen
Published: May 2018

Mixed metal oxides are promising heterogeneous catalysts for biofuel production from lipids via alcoholysis, however, the impact of solid acidity and/or basicity on reactivity is comparatively poorly understood. Two systematically related families of MgO–ZrO2 mixed oxide catalysts were therefore prepared by different synthetic routes to elucidate the impact of surface acid-base properties on catalytic performance in the transesterification of tributyrin with methanol. The resulting materials were characterized by TGA-MS, ICP-OES, N2 porosimetry, XRD, TEM, XPS, DRIFTS, and CO2-temperature-programmed desorption (TPD). MgO–ZrO2 catalysts prepared by both non-aqueous impregnation and citric acid-mediated sol–gel routes exhibit excellent activity and stability. The citrate routes favor highly dispersed MgO and concomitant Lewis acid-base pair formation at the interface with zirconia. However, for both the citrate and impregnation routes, tributyrin transesterification occurs over a common, strongly basic MgO active site.
Novel Modulators of Proteostasis: RNAi Screen of Chromosome I in a Heat Stress Paradigm in C. elegans
Authors: Kern Andreas , Spang Natalie , Huesmann Heike , Behl Christian
Published: May 2018

Proteostasis is of vital importance for cellular function and it is challenged upon exposure to acute or chronic insults during neurodegeneration and aging. The proteostasis network is relevant for the maintenance of proteome integrity and mainly comprises molecular chaperones and two degradation pathways, namely, autophagy and the ubiquitin proteasome system. This network is characterized by an impressive functional interrelation and complexity, and occasionally novel factors are discovered that modulate proteostasis. Here, we present an RNAi screen in C. elegans, which aimed to identify modulators of proteostasis in a heat stress paradigm. The screen comprised genes that are located on chromosome I of the nematode and has identified 185 genetic modifiers, whose knockdown has enhanced the misfolding of a reporter protein upon temperature increase. Subsequently, we evaluated the effect of a distinct number of the identified candidates in an additional C. elegans model strain, which expresses the aggregation-prone PolyQ35::YFP protein. Moreover, we annotated the human orthologs of the identified proteins and analyzed their enrichment in functional clusters and, as appropriate, their association with human neuropathologies. The achieved data collection includes several factors that have already been functionally associated with the proteostasis network, which highlights the potential of this heat stress-based proteostasis screen in order to detect novel modulators of proteome integrity.
Biodegradable Polymers Grafted onto Multifunctional Mesoporous Silica Nanoparticles for Gene Delivery
Authors: Nhavene Egídio Paulo Francisco , Andrade Gracielle Ferreira , Faria Jerusa Araujo Quintão Arantes , Gomes Dawidson Assis , Sousa Edésia Martins Barros de
Published: May 2018

Biodegradable polymer possesses significant potential for applications in different fields, since flexibility gives rise to materials with great physical and mechanical property diversity. The poly-caprolactone (PCL) and chitosan derivatives (CS) have the ability to form scaffolds, which adhere to the surface of mesoporous silica nanoparticles (MSNs) and its porous networks. The novel characteristics of the developed PCL/MSNs and CS/MSNs, such as very low in vivo degradation rate, ordered pore network, uniform and tunable size and shape of the particles, high pore volume and surface area, non-toxicity, and biocompatibility, among others, are responsible for its favorable gene delivery device and makes this conjugation a very good biomaterial for this application. In the present study, we investigated the synthesis of silica nanoparticles MCM-41 covalently grafted with PCL and CS and their use as a potential small interfering RNA (siRNA) carrier. The physical–chemical and morphological characterizations, as well as the applicability of functionalized MSNs as platforms for gene delivery, were assessed. Our results confirmed that MSNs that were successfully functionalized with PCL and CS kept their typical morphology and pore arrangement. Furthermore, their surface modification was successfully held. In vitro biocompatibility and cytotoxicity assays suggest the ability of MSNs to support passive uptake and indicated the potential of this material as a gene delivery system for cervical cancer cells (HeLa).
Osteogenesis and Antibacterial Activity of Graphene Oxide and Dexamethasone Coatings on Porous Polyetheretherketone via Polydopamine-Assisted Chemistry
Authors: Ouyang Ling , Qi Meiyao , Wang Shengnan , Tu Shan , Li Bogang , Deng Yi , Yang Weizhong
Published: May 2018

Endowing implants with antibacterial ability and osteogenic ability plays important roles in preventing post-operative bacterial contamination and facilitating integration between implants and osseous tissue, consequently reducing implant failure rates. In this study, we develop a facile and versatile strategy with dopamine as an auxiliary for construction of dexamethasone (Dex)/liposome porous coatings. In detail, the surfaces of sulfonated polyetheretherketone (SP) plates are coated with polydopamine firstly and then modified with graphene oxide (GO) and dexamethasone (Dex)-loaded liposome, which is verified by contact angle, X-ray photoelectron spectroscopy (XPS), attenuated total reflection infrared (ATR), and Raman spectra. The results of our study suggest that the GO and Dex are successfully coated on the samples’ surfaces. In vitro cell attachment, growth, differentiation, and apatite deposition tests all illustrate that the substrate coated with GO and Dex can significantly accelerate the proliferation and osteogenic differentiation of MC3T3 cells compared with the pristine sulfonated polyetheretherketone (PEEK). Additionally, it exhibits acceptable antibacterial activity against E. coli and S. aureus in vitro. Altogether, our results demonstrate that the modified GO- and Dex-loaded substrates are endowed with impressive biocompatibility and certain antibacterial qualities, making it possible for future application as a perspective implant material.
The Income Equalization System among Municipalities in Norway: Strengths and Implications
Authors: Idsø Johannes , Årethun Torbjørn , Bhatta Bharat P.
Published: May 2018

Norway is a leading nation pursuing egalitarian public policies. With an aim to smooth out income differences between municipalities and to stabilize individual municipality’s revenue over time, Norway has implemented a scheme called the income equalization system among municipalities. The scheme, which transfers revenue to poor municipalities, helps to maintain similar welfare services in all municipalities. We present and illustrate the model with data from all municipalities in Norway. We also demonstrate how the scheme helps stabilize tax revenue across municipalities over time. Furthermore, we show how the scheme can cause poor municipalities to have reduced incentives to increase their tax revenue.
Design of High-Security USB Flash Drives Based on Chaos Authentication
Authors: Liao Teh-Lu , Wan Pei-Yen , Chien Pin-Cheng , Liao Yi-Chieh , Wang Liang-Kai , Yan Jun-Juh
Published: May 2018

This paper aims to propose a novel design of high-security USB flash drives with the chaos authentication. A chaos authentication approach with the non-linear encryption and decryption function design is newly proposed and realized based on the controller design of chaos synchronization. To complete the design of high-security USB flash drives, first, we introduce six parameters into the original Henon map to adjust and obtain richer chaotic state responses. Then a discrete sliding mode scheme is proposed to solve the synchronization problem of discrete hyperchaotic Henon maps. The proposed sliding mode controller can ensure the synchronization of the master-slave Henon maps. The selection of the switching surface and the existence of the sliding motion are also addressed. Finally, the obtained results are applied to design a new high-security USB flash drive with chaos authentication. We built discrete hyperchaotic Henon maps in the smartphone (master) and microcontroller (slave), respectively. The Bluetooth module is used to communicate between the master and the slave to achieve chaos synchronization such that the same random and dynamical chaos signal can be simultaneously obtained at both the USB flash drive and smartphone, and pass the chaos authentication. When users need to access data in the flash drive, they can easily enable the encryption APP in the smartphone (master) for chaos authentication. After completing the chaos synchronization and authentication, the ARM-based microcontroller allows the computer to access the data in the high-security USB flash drive.
Magnesium Aminoclay-Fe3O4 (MgAC-Fe3O4) Hybrid Composites for Harvesting of Mixed Microalgae
Authors: Kim Bohwa , Bui Vu Khac Hoang , Farooq Wasif , Jeon Sang Goo , Oh You-Kwan , Lee Young-Chul
Published: May 2018

In this paper, we describe the synthesis of magnesium aminoclay-iron oxide (MgAC-Fe3O4) hybrid composites for microalgae-harvesting application. MgAC-templated Fe3O4 nanoparticles (NPs) were synthesized in different ratios of MgAC and Fe3O4 NPs. The uniform distribution of Fe3O4 NPs in the MgAC matrix was confirmed by transmission electron microscopy (TEM). According to obtained X-ray diffraction (XRD) patterns, increased MgAC loading leads to decreased intensity of the composites’ (311) plane of Fe3O4 NPs. For harvesting of Chlorella sp. KR-1, Scenedesmus obliquus and mixed microalgae (Chlorella sp. KR-1/ Scenedesmus obliquus), the optimal pH was 4.0. At higher pHs, the microalgae-harvesting efficiencies fell. Sample #1, which had the highest MgAC concentration, showed the most stability: the harvesting efficiencies for Chlorella sp. KR-1, Scenedesmus obliquus, and mixed microalgae were reduced only to ~50% at pH = 10.0. The electrostatic interaction between MgAC and the Fe3O4 NPs in the hybrid samples by microalgae, as confirmed by zeta potential measurements, were attributed to the harvesting mechanisms. Moreover, the zeta potentials of the MgAC-Fe3O4 hybrid composites were reduced as pH was increased, thus diminishing the microalgae-harvesting efficiencies.
Hybrid Decomposition-Reconfiguration Models for Long-Term Solar Radiation Prediction Only Using Historical Radiation Records
Authors: Wang Si-Ya , Qiu Jun , Li Fang-Fang
Published: May 2018

Solar radiation prediction is significant for solar energy utilization. This paper presents hybrid methods following the decomposition-prediction-reconfiguration paradigm using only historical radiation records with different combination of decomposition methods, Ensemble Empirical Mode Decomposition (EEMD) and Wavelet Analysis (WA), and the reconfiguration methods, regression model (RE) and Artificial Neural Network (ANN). The application in west China indicates that these hybrid decomposition-reconfiguration models perform well for monthly prediction, while the comparisons of the daily prediction show that the hybrid EEMD-RE model has a higher degree of fitting and a better prediction effect in long-term prediction of solar radiation intensity, which verifies (1) decomposition of original solar radiation data results in components with regular characteristics; (2) the relationship between the original solar radiation sequence and the derived intrinsic mode functions (IMFs) is linear; and (3) EEMD has strong adaptivity for non-linear and non-stationary series. The proposed hybrid decomposition-reconfiguration models have great application prospect for monthly long-term prediction of solar radiation intensity, especially in the areas where complex climate data is difficult to obtain, and the EEMD-RE model is recommended for the daily long-term prediction.
Changing the Urban Sound Environment in Greece: A Guide Based on Selected Case Studies of Strategic Noise Maps (SNM) and Noise Action Plans (NAP) in Medium and Large Urban Areas
Authors: Vogiatzis Konstantinos , Rémy Nicolas
Published: May 2018

Within the frame of European directive 2002/49 for Strategic Noise Mapping and the relevant environmental Noise Action Plans, which included preparation for the management of environmental noise and the rehabilitation of the sound environment, Greece had the opportunity to develop an innovative and comprehensive methodology to analyze the sound environments of several urban and semi-urban residential neighborhoods in the large and medium-size cities of the country e.g., Volos, Larissa, Heraklion, Chania, Agrinio, Corfu, and Thessaloniki, between 2012 and 2016. This paper presents the determined multidisciplinary approach, showing how the environmental noise data are cross-analyzed with urban and architectural data and perception descriptors by inhabitants. Furthermore, it shows how these specific results have been implemented in the developed noise actions plans that have been proposed to the authorities for immediate implementation. Finally, the paper discusses the necessary development of this approach to reduce noise exposure problems, as well as assist the cities in their evolution toward the introduction of a sustainable urban sound environment.
Analytical Solutions to Fractional Fluid Flow and Oscillatory Process Models
Authors: Zakariya Yusuf , Afolabi Yusuf , Nuruddeen Rahmatullah , Sarumi Ibrahim
Published: May 2018

In this paper, we provide solutions to the general fractional Caputo-type differential equation models for the dynamics of a sphere immersed in an incompressible viscous fluid and oscillatory process with fractional damping using Laplace transform method. We study the effects of fixing one of the fractional indices while varying the other as particular examples. We conclude this article by explaining the dynamics of the solutions of the models.
Planets, Planetary Nebulae, and Intermediate Luminosity Optical Transients (ILOTs)
Authors: Soker Noam
Published: May 2018

I review some aspects related to the influence of planets on the evolution of stars before and beyond the main sequence. Some processes include the tidal destruction of a planet on to a very young main sequence star, on to a low-mass main sequence star, and on to a brown dwarf. This process releases gravitational energy that might be observed as a faint intermediate luminosity optical transient (ILOT) event. I then summarize the view that some elliptical planetary nebulae are shaped by planets. When the planet interacts with a low-mass, upper asymptotic giant branch (AGB) star, it both enhances the mass-loss rate and shapes the wind to form an elliptical planetary nebula, mainly by spinning up the envelope and by exciting waves in the envelope. If no interaction with a companion, stellar or substellar, takes place beyond the main sequence, the star is termed a Jsolated star, and its mass-loss rates on the giant branches are likely to be much lower than what is traditionally assumed.
Identifying Degenerative Brain Disease Using Rough Set Classifier Based on Wavelet Packet Method
Authors: Cheng Ching-Hsue , Liu Wei-Xiang
Published: May 2018

Population aging has become a worldwide phenomenon, which causes many serious problems. The medical issues related to degenerative brain disease have gradually become a concern. Magnetic Resonance Imaging is one of the most advanced methods for medical imaging and is especially suitable for brain scans. From the literature, although the automatic segmentation method is less laborious and time-consuming, it is restricted in several specific types of images. In addition, hybrid techniques segmentation improves the shortcomings of the single segmentation method. Therefore, this study proposed a hybrid segmentation combined with rough set classifier and wavelet packet method to identify degenerative brain disease. The proposed method is a three-stage image process method to enhance accuracy of brain disease classification. In the first stage, this study used the proposed hybrid segmentation algorithms to segment the brain ROI (region of interest). In the second stage, wavelet packet was used to conduct the image decomposition and calculate the feature values. In the final stage, the rough set classifier was utilized to identify the degenerative brain disease. In verification and comparison, two experiments were employed to verify the effectiveness of the proposed method and compare with the TV-seg (total variation segmentation) algorithm, Discrete Cosine Transform, and the listing classifiers. Overall, the results indicated that the proposed method outperforms the listing methods.
Surgical and Rehabilitative Treatment of Misdiagnosed Posterior Dislocation of the Shoulder: Case Series
Authors: Pavone Vito , Caruso Vincenzo Fabrizio , Chisari Emanuele , Mangano Sebastiano , Costa Danilo , Sessa Giuseppe , Testa Gianluca
Published: May 2018

Background: Posterior gleno-humeral joint (GHJ) dislocation is a rare injury accounting for 2.7–3% of all shoulder dislocations. Early detection is the key for an effective treatment. Poor clinical outcomes or prosthetic solutions depend on the severity of the lesions at the time of trauma and worsening of the McLaughlin lesion during the months when the dislocation remains unrecognized or untreated. The aim of the study is to report our experience in treating, and clinically and radiographically evaluate the outcomes of, four neglected posterior GHJ dislocations. Methods: Four patients with inveterate posterior dislocation of the shoulder were evaluated. In three cases, the technique of McLaughlin was performed, and in one case, plication of the subscapularis tendon was performed. During the follow up, patients were clinically evaluated using the Constant shoulder score; an imaging investigation, using X-ray integrated with computed tomography(CT) and magnetic resonance imaging (MRI), was assessed. Results: Following the Constant criteria, treated shoulders reported an excellent outcome with an average score of 91. The mean score of untreated shoulders was 92.5. The humeral appearance that resulted was radiographically well-structured. Surgical treatment with McLaughlin lesion or transposition of the subscapularis were satisfactory from a subjective and objective point of view. To perform surgery soon as possible after the traumatic episode is considered a positive prognostic factor.
Ethyl 5-methyl-7-(4-morpholinophenyl)-4,7-dihydrotetrazolo[1,5-a]pyrimidine-6-carboxylate
Authors: Suwito Hery , Kurnyawaty Noorma , Ul Haq Kautsar , Abdulloh Abdulloh , Indriani Indriani
Published: May 2018

A new compound belonging to a dihydrotetrazolopyrimidine derivative, that is, ethyl 5-methyl-7-(4-morpholinophenyl)-4,7-dihydrotetrazolo[1,5-a]pyrimidine-6-carboxylate, was successfully synthesized using a Biginelli reaction between 4-morpholinobenzaldehyde, ethyl acetoacetate, and 5-aminotetrazole with p-toluenesulfonic acid (pTSA) as a catalyst in ethanol under reflux. The molecular structure of the title compound was characterized on the basis of spectroscopic evidence, using FTIR, HRESI-MS, 1H- and 13C-NMR, and 2D NMR.
Mechanistic Potential and Therapeutic Implications of Cannabinoids in Nonalcoholic Fatty Liver Disease
Authors: Dibba Pratima , Li Andrew , Cholankeril George , Iqbal Umair , Gadiparthi Chiranjeevi , Khan Muhammad Ali , Kim Donghee , Ahmed Aijaz
Published: May 2018

Nonalcoholic fatty liver disease (NAFLD) is comprised of nonalcoholic fatty liver (NAFL) and nonalcoholic steatohepatitis (NASH). It is defined by histologic or radiographic evidence of steatosis in the absence of alternative etiologies, including significant alcohol consumption, steatogenic medication use, or hereditary disorders. NAFLD is now the most common liver disease, and when NASH is present it can progress to fibrosis and hepatocellular carcinoma. Different mechanisms have been identified as contributors to the physiology of NAFLD; insulin resistance and related metabolic derangements have been the hallmark of physiology associated with NAFLD. The mainstay of treatment has classically involved lifestyle modifications focused on the reduction of insulin resistance. However, emerging evidence suggests that the endocannabinoid system and its associated cannabinoid receptors and ligands have mechanistic and therapeutic implications in metabolic derangements and specifically in NAFLD. Cannabinoid receptor 1 antagonism has demonstrated promising effects with increased resistance to hepatic steatosis, reversal of hepatic steatosis, and improvements in glycemic control, insulin resistance, and dyslipidemia. Literature regarding the role of cannabinoid receptor 2 in NAFLD is controversial. Exocannabinoids and endocannabinoids have demonstrated some therapeutic impact on metabolic derangements associated with NAFLD, although literature regarding direct therapeutic use in NAFLD is limited. Nonetheless, the properties of the endocannabinoid system, its receptors, substrates, and ligands remain a significant arena warranting further research, with potential for a pharmacologic intervention for a disease with an anticipated increase in economic and clinical burden.
ENABLE 2017, the First EUROPEAN PhD and Post-Doc Symposium. Session 4: From Discovery to Cure: The Future of Therapeutics
Authors: Di Mauro Gianmarco , Dondi Ambra , Giangreco Giovanni , Hogrebe Alexander , Louer Elja , Magistrati Elisa , Mullari Meeli , Turon Gemma , Verdurmen Wouter , Xicoy Cortada Helena , Zivanovic Sanja
Published: May 2018

The EUROPEAN ACADEMY FOR BIOMEDICAL SCIENCE (ENABLE) is an initiative funded by the European Union Horizon 2020 program involving four renowned European Research Institutes (Institute for Research in Biomedicine—IRB Barcelona, Spain; Radboud Institute for Molecular Life Sciences—RIMLS, the Netherlands; Novo Nordisk Foundation Center for Protein Research—NNF CPR, Denmark; European School of Molecular Medicine—SEMM, Italy) and an innovative science communication agency (Scienseed). With the aim of promoting biomedical science of excellence in Europe, ENABLE organizes an annual three-day international event. This gathering includes a top-level scientific symposium bringing together leading scientists, PhD students, and post-doctoral fellows; career development activities supporting the progression of young researchers and fostering discussion about opportunities beyond the bench; and outreach activities stimulating the interaction between science and society. The first European PhD and Postdoc Symposium, entitled “Breaking Down Complexity: Innovative Models and Techniques in Biomedicine”, was hosted by the vibrant city of Barcelona. The scientific program of the conference was focused on the most recent advances and applications of modern techniques and models in biomedical research and covered a wide range of topics, from synthetic biology to translational medicine. Overall, the event was a great success, with more than 200 attendees from all over Europe actively participating in the symposium by presenting their research and exchanging ideas with their peers and world-renowned scientists.
Low-Energy Membrane Process for Concentration of Stick Water
Authors: Zhou Jinxiang , Husson Scott M.
Published: May 2018

This communication describes the application of forward osmosis (FO) to concentrate stick water, a nutrient-rich water byproduct of meat rendering operations. The objectives of the study were to carry out a set of batch FO runs in concentration mode to determine the maximum achievable stick water concentration and to perform a preliminary cost analysis for operating a FO/reverse osmosis membrane separation process for comparison to an evaporative concentration process. The study examined the roles of feed and draw solution stir rates, temperature, feed concentration, and draw solution ionic strength on flux using commercial cellulose triacetate membranes. Results show that FO could concentrate the stick water up to 45 wt %; however, concentrations above about 30 wt % would be difficult to process through conventional membrane configurations. Preliminary operating cost estimations show that the energy cost of the FO process is about 5.3% of the energy costs for a single-effect thermal evaporation process; and, assuming a 2-year membrane lifetime, the total operating cost using FO membranes was estimated to be about 23.1% of the operating cost using such a thermal evaporation process.
Research on the Bonding Interface of High Speed Steel/Ductile Cast Iron Composite Roll Manufactured by an Improved Electroslag Cladding Method
Authors: Cao Yulong , Jiang Zhouhua , Dong Yanwu , Deng Xin , Medovar Lev , Stovpchenko Ganna
Published: May 2018

In the present study, a new electroslag cladding method by using of the advanced current supplying mold technology was used for manufacturing the high speed steel (HSS)/ductile cast iron (DCI) composite roll. The graphite morphology, matrix microstructure, elements distribution, carbides morphology, and carbides composition have been investigated by means of optical microscope (OM), scanning electron microscope (SEM), and energy dispersive spectroscopy (EDS). With increasing distance from the HSS side, a transition of graphite morphology from naught to existence and from small and dispersed to large and nonuniform was obtained at the interface. It was closely related to the fact that graphite in DCI participated in the phase change and the roll core surface and its nearby positions was heated to a high temperature by the liquid slag during the whole electroslag cladding process. Due to the combined effects of melting and elements diffusion, a significant migration of the alloying elements have occurred through the line scan analysis. Based on this, different types of carbides with the morphology and composition were found at the bonding interface. In addition, no obvious slag inclusions, porosity, shrinkage and other defects at the bonding interface were found. Results of the tensile test also illustrated that the bonding interface had a good quality and it could fully meet the requirements of the roll.
Research on PF-SLAM Indoor Pedestrian Localization Algorithm Based on Feature Point Map
Authors: Shi Jingjing , Ren Mingrong , Wang Pu , Meng Juan
Published: May 2018

Recently, the map matching-assisted positioning method based on micro-electromechanical systems (MEMS) inertial devices has become a research hotspot for indoor pedestrian positioning; however, these are based on existing indoor electronic maps. In this paper, without prior knowledge of the map and through building an indoor main path feature point map combined with the simultaneous localization and map building (SLAM) particle filter (PF-SLAM) algorithm idea, a PF-SLAM indoor pedestrian location algorithm based on a feature point map was proposed through the inertial measurement unit to improve indoor pedestrian positioning accuracy. Aiming at the problem of inaccurate heading angle estimation in the pedestrian dead reckoning (PDR) algorithm, a turn-straight-state threshold detection method was proposed that corrected the difference of the heading angles during the straight-line walking of pedestrians to suppress the error accumulation of the heading angle. Aiming at the particles that are severely divergent at the corners, a feature point matching algorithm was proposed to correct the pedestrian position error. Furthermore, the turning point extracted the main path that failed to match the current feature point map as a new feature point was added to update the map. Through the mutual modification of SLAM and an inertial navigation system (INS) the long-time, high-precision, and low-cost positioning functions of indoor pedestrians were realized.
One-Step Synthesis of Au-Ag Nanowires through Microorganism-Mediated, CTAB-Directed Approach
Authors: Xu Luhang , Huang Dengpo , Chen Huimei , Jing Xiaoling , Huang Jiale , Odoom-Wubah Tareque , Li Qingbiao
Published: May 2018

Synthesis and applications of one dimensional (1D) metal nanostructures have attracted much attention. However, one-step synthesis of bimetallic nanowires (NWs) has remained challenging. In this work, we developed a microorganism-mediated, hexadecyltrimethylammonium bromide (CTAB)-directed (MCD) approach to synthesize closely packed and long Au-Ag NWs with the assistance of a continuous injection pump. Characterization results confirmed that the branched Au-Ag alloy NWs was polycrystalline. And the Au-Ag NWs exhibited a strong absorbance at around 1950 nm in the near-infrared (NIR) region, which can find potential application in NIR absorption. In addition, the Au-Ag NWs showed excellent surface-enhanced Raman scattering (SERS) enhancement when 4-mercaptobenzoic acid (MBA) and rhodamine 6G (R6G) were used as probe molecules.
Functional Role of Non-Coding RNAs during Epithelial-To-Mesenchymal Transition
Authors: Expósito-Villén Almudena , E. Aránega Amelia , Franco Diego
Published: May 2018

Epithelial-to-mesenchymal transition (EMT) is a key biological process involved in a multitude of developmental and pathological events. It is characterized by the progressive loss of cell-to-cell contacts and actin cytoskeletal rearrangements, leading to filopodia formation and the progressive up-regulation of a mesenchymal gene expression pattern enabling cell migration. Epithelial-to-mesenchymal transition is already observed in early embryonic stages such as gastrulation, when the epiblast undergoes an EMT process and therefore leads to the formation of the third embryonic layer, the mesoderm. Epithelial-to-mesenchymal transition is pivotal in multiple embryonic processes, such as for example during cardiovascular system development, as valve primordia are formed and the cardiac jelly is progressively invaded by endocardium-derived mesenchyme or as the external cardiac cell layer is established, i.e., the epicardium and cells detached migrate into the embryonic myocardial to form the cardiac fibrous skeleton and the coronary vasculature. Strikingly, the most important biological event in which EMT is pivotal is cancer development and metastasis. Over the last years, understanding of the transcriptional regulatory networks involved in EMT has greatly advanced. Several transcriptional factors such as Snail, Slug, Twist, Zeb1 and Zeb2 have been reported to play fundamental roles in EMT, leading in most cases to transcriptional repression of cell–cell interacting proteins such as ZO-1 and cadherins and activation of cytoskeletal markers such as vimentin. In recent years, a fundamental role for non-coding RNAs, particularly microRNAs and more recently long non-coding RNAs, has been identified in normal tissue development and homeostasis as well as in several oncogenic processes. In this study, we will provide a state-of-the-art review of the functional roles of non-coding RNAs, particularly microRNAs, in epithelial-to-mesenchymal transition in both developmental and pathological EMT.
Longitudinal Study of the Role of Epidermal Growth Factor on the Fractional Excretion of Magnesium in Children: Effect of Calcineurin Inhibitors
Authors: Ledeganck Kristien J. , Anné Charlotte , De Monie Amandine , Meybosch Sarang , Verpooten Gert A. , Vinckx Marleen , Van Hoeck Koen , Van Eyck Annelies , De Winter Benedicte Y. , Trouet Dominique
Published: May 2018

Background: It was shown in animal models and adults that the epidermal growth factor (EGF) is involved in the pathophysiology of calcineurin inhibitor (CNI) induced renal magnesium loss. In children, however, the exact mechanism remains unclear, which was set as the purpose of the present study. Methods: Children with nephrotic syndrome and renal transplant children treated with CNI (n = 50) and non-CNI treated children (n = 46) were included in this study. Urine and serum samples were collected at three time points to determine magnesium, creatinine, and EGF. The magnesium intake was calculated from a food frequency questionnaire. Results: Serum Mg2+ and urinary EGF/creatinine were significantly lower in the CNI treated children, with significantly more CNI-treated children developing hypomagnesaemia. In the latter patients, the fractional excretion of magnesium (FE Mg2+) was significantly higher. Urinary EGF, age, renal function, and serum magnesium were independent predictors of the FE Mg2+. Only 29% of the children reached the recommended daily intake of magnesium. The magnesium intake did not differ between hypomagnesemic and normomagnesemic patients and was not a predictor of the FE Mg2+. Conclusions: In CNI-treated children who developed hypomagnesemia, the FE Mg2+ was increased. The urinary EGF concentration, age, and renal function are independent predictors of the FE Mg2+.
Small Intestinal Permeability and Gut-Transit Time Determined with Low and High Molecular Weight Fluorescein Isothiocyanate-Dextrans in C3H Mice
Authors: Woting Anni , Blaut Michael
Published: May 2018

Protocols for intestinal permeability measurements in mice using 4-kDa fluorescein isothiocyanate-conjugated (FITC) dextran differ considerably among laboratories on the blood-sampling time. To find the optimal point in time for blood sampling, we administered 4-kDa FITC dextran to C3H mice and monitored the marker in plasma over 8 h. We also determined gut-transit time using 70-kDa FITC dextran, which does not cross the intestinal epithelium. The 4-kDa FITC dextran concentration in plasma reached its maximum 45 min after administration. The 70-kDa FITC dextran reached the jejunum after 15 min and passed the entire small intestine within 1 h after its administration, demonstrating that 4-kDa FITC dextran measured in plasma 1 h after its oral application is a marker of small intestinal permeability.
Effect of Fucoidan on Anterior Cruciate Ligament Transection and Medial Meniscectomy Induced Osteoarthritis in High-Fat Diet-Induced Obese Rats
Authors: Sudirman Sabri , Ong Alan Darmasaputra , Chang Heng-Wei , Kong Zwe-Ling
Published: May 2018

Osteoarthritis (OA) has become one of the most common disabilities among elders, especially in females. Obesity and mechanical injuries caused by OA are attributed to joint loading, cartilage disintegration, and bone loss, as well as inflammation. Pharmacological and non-pharmacological treatments can be used for OA. Fucoidan possesses several bioactivities such as antitumor, antiviral, anticoagulation, anti-obesity, and immunomodulation. This study aims to investigate the effect of fucoidan in surgery-induced OA on rats with diet-induced obesity. OA was induced by an anterior cruciate ligament transection and a partial medial meniscectomy (ACLT + MMx). The male SD rats were fed with a high-fat diet (HFD) for 4 weeks to induce obesity before causing ACLT + MMx to induce OA. The OA rats were administered with intragastric water or fucoidan in three different concentrations (32 mg/kg, 64 mg/kg, and 320 mg/kg) after the surgeries for 40 days with an HFD. We observed that the swelling in the knee joint was alleviated and the hind paw weight distribution was rectified after feeding them with fucoidan and that there was no significant effect on the weight gain and feed intake. Fucoidan administration indicated no significant variation on the high-density lipoprotein (HDL)-Cholesterol level, but it did indicate reduced plasma triglycerides and low-density lipoprotein (LDL)-Cholesterol levels. In addition, the weight-bearing tests showed an improvement in the fucoidan-treated group. Our results suggested that fucoidan may improve meniscal/ligamentous injury and obesity-induced OA.
Maternal Circulating Vitamin Status and Colostrum Vitamin Composition in Healthy Lactating Women—A Systematic Approach
Authors: Y. de Vries Jasmijn , Pundir Shikha , Mckenzie Elizabeth , Keijer Jaap , Kussmann Martin
Published: May 2018

Colostrum is the first ingested sole nutritional source for the newborn infant. The vitamin profile of colostrum depends on the maternal vitamin status, which in turn is influenced by diet and lifestyle. Yet, the relationship between maternal vitamin status and colostrum vitamin composition has not been systematically reviewed. This review was conducted with the aim to generate a comprehensive overview on the relationship between maternal serum (plasma) vitamin concentration and corresponding colostrum composition. Three electronic databases, Embase (Ovid), Medline (Ovid), and Cochrane, were systematically searched based on predefined inclusion and exclusion criteria. Finally, a total of 11 eligible publications were included that examined the vitamins A, C, D, E, and K in both biological fluids. Maternal vitamin A, D, E, and K blood levels were unrelated to colostrum content of the respective vitamins, and serum vitamin A was inversely correlated with colostrum vitamin E. Colostrum versus maternal serum vitamins were higher for vitamins A, C, and K, lower for vitamin D, and divergent results were reported for vitamin E levels. Colostrum appears typically enriched in vitamin A, C, and K compared to maternal serum, possibly indicative of active mammary gland transport mechanisms. Inter-individual and inter-study high variability in colostrum’s vitamin content endorses its sensitivity to external factors.
Are Front-of-Package Warning Labels More Effective at Communicating Nutrition Information than Traffic-Light Labels? A Randomized Controlled Experiment in a Brazilian Sample
Authors: Khandpur Neha , Sato Priscila de Morais , Mais Laís Amaral , Martins Ana Paula Bortoletto , Spinillo Carla Galvão , Garcia Mariana Tarricone , Rojas Carlos Felipe Urquizar , Jaime Patrícia Constante
Published: May 2018

Background: Brazil is currently debating the implementation of front-of-package labels. This study tested if Warning labels (WLs) improved consumer understanding, perceptions, and purchase intentions compared to Traffic-Light labels (TLLs) in 1607 Brazilian adults. Methods: In this online, randomized controlled experiment participants saw images of 10 products and answered questions twice—once in a no-label, control condition and then again in a randomly assigned label condition. The relative differences in responses between WLs and TLLs between control and label conditions were estimated using one-way ANOVAs or Chi-square tests. Results: Presenting WLs on products compared to TLLs helped participants: (i) improve their understanding of excess nutrient content (27.0% versus 8.2%, p < 0.001); (ii) improve their ability to identify the healthier product (24.6% versus 3.3%, p < 0.001); (iii) decrease perceptions of product healthfulness; and (iv) correctly identify healthier products (14.0% versus 6.9%, p < 0.001), relative to the control condition. With WLs, there was also an increase in the percentage of people: (v) expressing an intention to purchase the relatively healthier option (16.1% versus 9.8%, p < 0.001); and (vi) choosing not to buy either product (13.0% versus 2.9%, p < 0.001), relative to the control condition. The participants in the WL condition had significantly more favorable opinions of the labels compared to those in the TLL group. Conclusions: WLs would be more effective, compared to the TLL, at improving consumer food choices.