bluuebunny/crossref_metadata_2025 huggingface dataset:
Over-Expression of a Melon Y3SK2-Type LEA Gene Confers Drought and Salt Tolerance in Transgenic Tobacco Plants
Authors: Aduse Poku Samuel , Nkachukwu Chukwurah Peter , Aung Htut Htet , Nakamura Ikuo
Published: December 2020

Climate change, with its attendant negative effects, is expected to hamper agricultural production in the coming years. To counteract these negative effects, breeding of environmentally resilient plants via conventional means and genetic engineering is necessary. Stress defense genes are valuable tools by which this can be achieved. Here we report the successful cloning and functional characterization of a melon Y3SK2-type dehydrin gene, designated as CmLEA-S. We generated CmLEA-S overexpressing transgenic tobacco lines and performed in vitro and in vivo drought and salt stress analyses. Seeds of transgenic tobacco plants grown on 10% polyethylene glycol (PEG) showed significantly higher germination rates relative to wild-type seeds. In the same way, transgenic seeds grown on 150 mM sodium chloride (NaCl) recorded significantly higher germination percentages compared with wild-type plants. The fresh weights and root lengths of young transgenic plants subjected to drought stress were significantly higher than that of wild-type plants. Similarly, the fresh weights and root lengths of transgenic seedlings subjected to salt stress treatments were also significantly higher than wild-type plants. Moreover, transgenic plants subjected to drought and salt stresses in vivo showed fewer signs of wilting and chlorosis, respectively. Biochemical assays revealed that transgenic plants accumulated more proline and less malondialdehyde (MDA) compared with wild-type plants under both drought and salt stress conditions. Finally, the enzymatic activities of ascorbate peroxidase (APX) and catalase (CAT) were enhanced in drought- and salt-stressed transgenic lines. These results suggest that the CmLEA-S gene could be used as a potential candidate gene for crop improvement.
Exploring the Mechanism of Gyejibokryeong-hwan against Atherosclerosis Using Network Pharmacology and Molecular Docking
Authors: Lee A Yeong , Lee Joo-Youn , Chun Jin Mi
Published: December 2020

Gyejibokryeong-hwan (GBH) is a traditional formula comprised of five herbal medicines that is frequently used to treat blood stasis and related complex multifactorial disorders such as atherosclerosis. The present study used network pharmacology and molecular docking simulations to clarify the effect and mechanism of the components of GBH. Active compounds were selected using Oriental Medicine Advanced Searching Integrated System (OASIS) and the Traditional Chinese Medicine System Pharmacology Database and Analysis Platform (TCMSP), and target genes linked to the selected components were retrieved using Search Tool for Interacting Chemicals (STITCH) and GeneCards. Functional analysis of potential target genes was performed through the Annotation, Visualization and Integrated Discovery (DAVID) database and the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway, and molecular docking confirmed the correlation between five core compounds (quercetin, kaempferol, baicalein, ellagic acid, and baicalin) and six potential target genes (AKT1, CASP3, MAPK1, MAPK3, NOS2, and PTGS2). Molecular docking studies indicated that quercetin strongly interacted with six potential target proteins. Thus, these potential target proteins were closely related to TNF, HIF-1, FoxO, and PI3K-Akt signal pathways, suggesting that these factors and pathways may mediate the beneficial effects of GBH on atherosclerosis. Our results identify target genes and pathways that may mediate the clinical effects of the compounds contained within GBH on atherosclerosis.
Nutritional Evaluation of Beetroots (Beta vulgaris L.) and Its Potential Application in a Functional Beverage
Authors: Abdo Eman , El-Sohaimy Sobhy , Shaltout Omayma , Abdalla Ahmed , Zeitoun Ahmed
Published: December 2020

Beetroot is a good source of minerals, fibers, and bioactive components. The present research work was conducted to evaluate the nutritional quality of beetroots (juice, peels, leaves and pomace) enhancing the extracted bioactive components, and developing a functional probiotic beverage. Chemical composition and minerals content of beetroot parts were estimated. The bioactive components were extracted by instant extraction method (IEM) and overnight extraction method (at −20 °C) (OEM) to determine total phenolics, flavonoids, and DPPH inhibition ratio. The extracted beetroot juice was mixed with milk for valorization of the beverage nutritional value and fermented with LA-5 and ABT-5 cultures to create a novel functional beverage. Chemical composition, minerals content, and bioactive components of beverages were estimated. The leaves exhibited the highest calcium content (1200 mg/100 g). Juice showed the highest amount of all minerals except for calcium and magnesium. Overnight extraction method (OEM) increased the antioxidant activity in peels and stems. Natural juice exhibited the highest activity compared to extracts. Fermentation of beet-milk beverage with LA-5 and ABT-5 cultures enhanced the beverage taste, flavor, and antioxidant capacity. Beetroot wastes and juice comprise a valuable nutritional source. Fermentation improved the nutritional value of beetroot and the acceptability of the product.
Recovery of Bioactive Compounds from Jaboticaba Peels and Application into Zein Ultrafine Fibers Produced by Electrospinning
Authors: Avila Luisa Bataglin , Fontes Milena Ramos Vaz , Zavareze Elessandra da Rosa , Moraes Caroline Costa , Morais Marcilio Machado , Rosa Gabriela Silveira da
Published: December 2020

This work focused on the recovery bioactive compounds from jaboticaba peels and to develop ultrafine fibers from zein incorporated with the jaboticaba extract by electrospinning technique. Jaboticaba peel extracts (JPE) were obtained by maceration according a central composite rotational design (CCDR) and characterized with respect to total phenolic content (TP), antioxidant activity (AA) and total anthocyanin (TA). The optimal condition for the extraction was obtained using a desirability function in order to maximize the presence of bioactive compounds. Under these conditions the amount of cyanidin-3-glucoside (Cn-3-Glu) and the antimicrobial inhibition (AI) of E. coli were evaluated. Ultrafine fibers were obtained by electrospinning technique using zein in an aqueous ethanol as solvent and freeze-dried JPE at different concentrations (1.7% and 3.3%) to produce a composite membrane. The apparent viscosity and electrical conductivity of the polymer solutions, as well as the morphology, thermal stability and functional groups of the ultrafine fibers, were evaluated. The optimal conditions for extraction were 88 °C and pH 1. Under these conditions, a high amount of Cn-3-Glu was obtained (718.12 mg 100 g−1), along with 22.2% antimicrobial inhibition against E. coli. The addition of JPE into composite membranes did not affect the morphology of fibers, which presented a homogeneous and continuous format. Therefore, fibers containing JPE showed interesting characteristics for the food packaging industry.
Hydrothermally Assisted Synthesis of Porous Polyaniline@Carbon Nanotubes–Manganese Dioxide Ternary Composite for Potential Application in Supercapattery
Authors: Iqbal Javed , Ansari Mohammad Omaish , Numan Arshid , Wageh S. , Al-Ghamdi Ahmed , Alam Mohd Gulfam , Kumar Pramod , Jafer Rashida , Bashir Shahid , Rajpar A. H.
Published: December 2020

In this study, ternary composites of polyaniline (PANI) with manganese dioxide (MnO2) nanorods and carbon nanotubes (CNTs) were prepared by employing a hydrothermal methodology and in-situ oxidative polymerization of aniline. The morphological analysis by scanning electron microscopy showed that the MnO2 possessed nanorod like structures in its pristine form, while in the ternary PANI@CNT/MnO2 composite, coating of PANI over CNT/MnO2, rods/tubes were evidently seen. The structural analysis by X-ray diffraction and X-ray photoelectron spectroscopy showed peaks corresponding to MnO2, PANI and CNT, which suggested efficacy of the synthesis methodology. The electrochemical performance in contrast to individual components revealed the enhanced performance of PANI@CNT/MnO2 composite due to the synergistic/additional effect of PANI, CNT and MnO2 compared to pure MnO2, PANI and PANI@CNT. The PANI@CNT/MnO2 ternary composite exhibited an excellent specific capacity of 143.26 C g−1 at a scan rate of 3 mV s−1. The cyclic stability of the supercapattery (PANI@CNT/MnO2/activated carbon)—consisting of a battery type electrode—demonstrated a gradual increase in specific capacity with continuous charge–discharge over ~1000 cycles and showed a cyclic stability of 119% compared to its initial value after 3500 cycles.
Self-Assembly of Molecular Brushes with Polyimide Backbone and Amphiphilic Block Copolymer Side Chains in Selective Solvents
Authors: Simonova Maria , Ivanov Ivan , Meleshko Tamara , Kopyshev Alexey , Santer Svetlana , Yakimansky Alexander , Filippov Alexander
Published: December 2020

Three-component molecular brushes with a polyimide backbone and amphiphilic block copolymer side chains with different contents of the “inner” hydrophilic (poly(methacrylic acid)) and “outer” hydrophobic (poly(methyl methacrylate)) blocks were synthesized and characterized by molecular hydrodynamics and optics methods in solutions of chloroform, dimethylformamide, tetrahydrofuran and ethanol. The peculiarity of the studied polymers is the amphiphilic structure of the grafted chains. The molar masses of the molecular brushes were determined by static and dynamic light scattering in chloroform in which polymers form molecularly disperse solutions. Spontaneous self-assembly of macromolecules was detected in dimethylformamide, tetrahydrofuran and ethanol. The aggregates size depended on the thermodynamic quality of the solvent as well as on the macromolecular architectural parameters. In dimethylformamide and tetrahydrofuran, the distribution of hydrodynamic radii of aggregates was bimodal, while in ethanol, it was unimodal. Moreover, in ethanol, an increase in the poly(methyl methacrylate) content caused a decrease in the hydrodynamic radius of aggregates. A significant difference in the nature of the blocks included in the brushes determines the selectivity of the used solvents, since their thermodynamic quality with respect to the blocks is different. The macromolecules of the studied graft copolymers tend to self-organization in selective solvents with formation of a core–shell structure with an insoluble solvophobic core surrounded by the solvophilic shell of side chains.
Numerical Modeling of Ink Widening and Coating Gap in Roll-to-Roll Slot-Die Coating of Solid Oxide Fuel Cell Electrolytic Layer
Authors: Kim Seongyong , Lee Jongsu , Jo Minho , Lee Changwoo
Published: December 2020

Slot-die coatings are advantageous when used for coating large-area flexible devices; in particular, the coating width can be controlled and simultaneous multi-layer coatings can be processed. To date, the effects of ink widening and the coating gap on the coating thickness have only been considered in a few studies. To this end, we developed two mathematical models to accurately estimate the coating width and thickness that consider these two effects. We used root mean square deviation (RMSD) to experimentally verify the developed method. When the coating gap was increased, the coating width increased and the coating thickness decreased. Experimental results showed that the estimated performances of the coating width and thickness models were as high as 98.46% and 95.8%, respectively. We think that the developed models can be useful for determining the coating conditions according to the ink properties to coat a functional layer with user-defined widths and thicknesses in both lab- and industrial-scale roll-to-roll slot-die coating processes.
Drug-Eluting Biodegradable Implants for the Sustained Release of Bisphosphonates
Authors: Dharmayanti Cintya , Gillam Todd A. , Williams Desmond B. , Blencowe Anton
Published: December 2020

Despite being one of the first-line treatments for osteoporosis, the bisphosphonate drug class exhibits an extremely low oral bioavailability (<1%) due to poor absorption from the gastrointestinal tract. To overcome this, and to explore the potential for sustained drug release, bioerodible poly(lactic acid) (PLA) and poly(D,L-lactide-co-glycolide) (PLGA) implants loaded with the bisphosphonate alendronate sodium (ALN) were prepared via hot-melt extrusion. The rate of drug release in vitro was modulated by tailoring the ratio of lactide to glycolide in the polymer and by altering the ALN-loading of the implants. All investigated implants exhibited sustained ALN release in vitro between 25 to 130 days, where implants of greater glycolide composition and higher ALN-loadings released ALN more rapidly. All PLGA implants demonstrated a sigmoidal release profile, characterised by an initial surface dissolution phase, followed by a period of zero-order drug diffusion, then relaxation or erosion of the polymer chains that caused accelerated release over the subsequent days. Contrastingly, the PLA implants demonstrated a logarithmic release profile, characterised by a gradual decrease in ALN release over time.
Multifunctional Properties of Binary Polyrhodanine Manganese Ferrite Nanohybrids—From the Energy Converters to Biological Activity
Authors: Zachanowicz Emilia , Kulpa-Greszta Magdalena , Tomaszewska Anna , Gazińska Małgorzata , Marędziak Monika , Marycz Krzysztof , Pązik Robert
Published: December 2020

The PRHD@MnFe2O4 binary hybrids have shown a potential for applications in the biomedical field. The polymer cover/shell provides sufficient surface protection of magnetic nanoparticles against adverse effects on the biological systems, e.g., it protects against Fenton’s reactions and the generation of highly toxic radicals. The heating ability of the PRHD@MnFe2O4 was measured as a laser optical density (LOD) dependence either for powders as well as nanohybrid dispersions. Dry hybrids exposed to the action of NIR radiation (808 nm) can effectively convert energy into heat that led to the enormous temperature increase ΔT 170 °C (>190 °C). High concentrated colloidal suspensions (5 mg/mL) can generate ΔT of 42 °C (65 °C). Further optimization of the nanohybrids amount and laser parameters provides the possibility of temperature control within a biologically relevant range. Biological interactions of PRHD@MnFe2O4 hybrids were tested using three specific cell lines: macrophages (RAW 264.7), osteosarcoma cells line (UMR-106), and stromal progenitor cells of adipose tissue (ASCs). It was shown that the cell response was strongly dependent on hybrid concentration. Antimicrobial activity of the proposed composites against Escherichia coli and Staphylococcus aureus was confirmed, showing potential in the exploitation of the fabricated materials in this field.
A Novel Silicon/Phosphorus Co-Flame Retardant Polymer Electrolyte for High-Safety All-Solid-State Lithium Ion Batteries
Authors: Zeng Li , Jia Lu , Liu Xingang , Zhang Chuhong
Published: December 2020

Developing a solid polymer electrolyte with superior flame retardancy and lithium-ion transportation properties is still a challenge. Herein, an intrinsic silicon/phosphorus co-flame retardant polymer solid electrolyte was prepared by using polyethylene glycol (PEG) co-polymerized with silicon and phosphorus-containing monomers. Due to the synergistic flame-retardant effect of silicon and phosphorus elements, this polymer electrolyte exhibits excellent thermal stability and flame resistance. Moreover, the ionic conductivity of the electrolyte at 25 °C is as high as 2.98 × 10−5 S/cm when the mass ratio of LiN(SO2CF3)2 (LiTFSI) and the prepared polymer electrolyte is 10:1. What is more, the LiFePO4/Li all-solid-state battery assembled with this solid electrolyte can work stably at a high temperature of 60 °C and exhibits a specific capacity of 129.2 mAh/g at 0.2 C after 100 cycles, providing a promising application prospect for high-safety lithium-ion batteries.
Biocompatibility and Physico-Chemical Properties of Highly Porous PLA/HA Scaffolds for Bone Reconstruction
Authors: Zimina Anna , Senatov Fedor , Choudhary Rajan , Kolesnikov Evgeniy , Anisimova Natalya , Kiselevskiy Mikhail , Orlova Polina , Strukova Natalia , Generalova Mariya , Manskikh Vasily , Gromov Alexander , Karyagina Anna
Published: December 2020

The major problem in bone tissue engineering is the development of scaffolds which can simultaneously meet the requirements of porous structure, as well as have the ability to guide the regeneration of damaged tissue by biological fixation. Composites containing biodegradable matrix and bioactive filler are the new hope in this research field. Herein we employed a simple and facile solvent casting particulate-leaching method for producing polylactide acid/hydroxyapatite (PLA/HA) composites at room temperature. FT-IR analysis confirmed the existence of necessary functional groups associated with the PLA/HA composite, whereas energy-dispersive X-ray (EDX) spectra indicated the uniform distribution of hydroxyapatite particles in the polymer matrix. The beehive-like surface morphology of the composites revealed the presence of macropores, ranged from 300 to 400 μm, whereas the thickness of the pores was noticed to be 1–2 μm. The total porosity of the scaffolds, calculated by hydrostatic weighing, was found to be 79%. The water contact angle of pure PLA was decreased from 83.6 ± 1.91° to 62.4 ± 4.17° due to the addition of hydroxyapatite in the polymer matrix. Thus, the wettability of the polymeric biomaterial could be increased by preparing their composites with hydroxyapatite. The adhesion of multipotent mesenchymal stromal cells over the surface of PLA/HA scaffolds was 3.2 times (p = 0.03) higher than the pure PLA sample. Subcutaneous implantation in mice demonstrated a good tolerance of all tested porous scaffolds and widespread ingrowth of tissue into the implant pores. HA-containing scaffolds showed a less pronounced inflammatory response after two weeks of implantation compared to pure PLA. These observations suggest that PLA/HA composites have enormous potential for hard tissue engineering and restoring maxillofacial defects.
Preparation and Characterization of a Composite Dust Suppressant for Coal Mines
Authors: Jin Hu , Zhang Yansong , Chen Kun , Niu Kuo , Wu Guangan , Wei Xiangrui , Wang Houwang
Published: December 2020

In an effort to effectively control coal dust pollution and thereby reduce the harm of coal dust to human health, we prepared a highly efficient composite dust suppressant. First, dynamic contact angle and zeta potential measurements were used to select sodium dodecyl sulfonate (SDS) over sodium carboxymethyl cellulose and trisodium methyl silicon as the complementary additive to soy protein isolate for the dust suppressant. We employed viscosity and wind erosion resistance tests to compare the performance of the composite dust suppressant with three common, commercially available suppressants. As the concentration of the composite dust suppressant was increased, the viscosity increased, reaching a maximum value of 22.7 mPa·s at a concentration of 5 wt%. The 5 wt% concentration of the composite dust suppressant provided the lowest wind erosion rate (20.62%) at a wind speed of 12 m/s. The composite dust suppressant also had good bonding performance and wind erosion resistance. Scanning electron microscopy, X-ray diffraction, and thermogravimetric analysis were used to characterize the properties of the dust suppressants. The dust suppressant, which had a crystal-like structure, could easily capture coal dust and form an effective package. In addition, the density of the dust suppressant film increased as its crystallinity increased. The increased density was beneficial in that it enabled the dust suppressant to form a hard, solidified shell on the surface of coal dust, which improved dust suppression. The composite dust suppressant also had good thermal stability.
Quasi-Static Shear Test of Hybrid Adhesive Bonds Based on Treated Cotton-Epoxy Resin Layer
Authors: Tichý Martin , Kolář Viktor , Müller Miroslav , Mishra Rajesh Kumar , Šleger Vladimír , Hromasová Monika
Published: December 2020

This research evaluates the mechanical properties of hybrid adhesive bonds with various 100% cotton fabrics in static and quasi-static conditions and the influence of alkali surface treatment (NaOH) of the cotton fabrics on the mechanical properties. Biological fibers in polymers are characterized by low wettability with the matrix, which decreases mechanical properties. Adhesive bonds usually operate in cyclic stress, which causes irreversible failure before maximal strength. In this paper, a quasi-static test was used to load the adhesive bonds in 5–50% (192–1951 N) and 5–70% (192–2732 N) intervals with 1000 cycles. The results of SEM analysis showed good wettability of alkali treated cotton fabric with NaOH solution in hybrid adhesive bonds. The static test proved the influence of reinforcing cotton fabrics on shear tensile strength against pure resin, i.e., sample Erik up to 19% on 14.90 ± 1.15 MPa and sample Tera up to 21% on 15.28 ± 1.05 MPa. The adhesive bonds with pure resin did not resist either quasi-static tests. Reinforcing cotton fabrics resisted both quasi-static tests, even shear tensile strength increases up to 10% on 16.34 ± 1.24 MPa for the fabric Erik. The results of strain difference of adhesive bonds with Tera and Erik confirmed that a lower value of the difference during cyclic loading positively influenced the ultimate shear tensile strength.
Flexural Strength of Resin Core Build-Up Materials: Correlation to Root Dentin Shear Bond Strength and Pull-Out Force
Authors: Irie Masao , Maruo Yukinori , Nishigawa Goro , Yoshihara Kumiko , Matsumoto Takuya
Published: December 2020

The aims of this study were to investigate the effects of root dentin shear bond strength and pull-out force of resin core build-up materials on flexural strength immediately after setting, after one-day water storage, and after 20,000 thermocycles. Eight core build-up and three luting materials were investigated, using 10 specimens (n = 10) per subgroup. At three time periods—immediately after setting, after one-day water storage, and after 20,000 thermocycles, shear bond strengths to root dentin and pull-out forces were measured. Flexural strengths were measured using a 3-point bending test. For all core build-up and luting materials, the mean data of flexural strength, shear bond strength and pull-out force were the lowest immediately after setting. After one-day storage, almost all the materials yielded their highest results. A weak, but statistically significant, correlation was found between flexural strength and shear bond strength (r = 0.508, p = 0.0026, n = 33). As the pull-out force increased, the flexural strength of core build-up materials also increased (r = 0.398, p = 0.0218, n = 33). Multiple linear regression analyses were conducted using these three independent factors of flexural strength, pull-out force and root dentin shear bond strength, which showed this relationship: Flexural strength = 3.264 × Shear bond strength + 1.533 × Pull out force + 10.870, p = 0.002). For all the 11 core build-up and luting materials investigated immediately after setting, after one-day storage and after 20,000 thermocycles, their shear bond strengths to root dentin and pull-out forces were correlated to the flexural strength in core build-up materials. It was concluded that the flexural strength results of the core build-up material be used in research and quality control for the predictor of the shear bond strength to the root dentin and the retentive force of the post.
Influence of Hydroxyapatite Nanospheres in Dentin Adhesive on the Dentin Bond Integrity and Degree of Conversion: A Scanning Electron Microscopy (SEM), Raman, Fourier Transform-Infrared (FTIR), and Microtensile Study
Authors: Al-Hamdan Rana S , Almutairi Basil , Kattan Hiba F , Alsuwailem Noura A. , Farooq Imran , Vohra Fahim , Abduljabbar Tariq
Published: December 2020

An experimental adhesive incorporated with different nano-hydroxyapatite (n-HA) particle concentrations was synthesized and analyzed for dentin interaction, micro-tensile bond strength (μTBS), and degree of conversion (DC). n-HA powder (5 wt % and 10 wt %) were added in adhesive to yield three groups; gp-1: control experimental adhesive (CEA, 0 wt % HA), gp-2: 5 wt % n-HA (HAA-5%), and gp-3: 10 wt % n-HA (HAA-10%). The morphology of n-HA spheres was evaluated using Scanning Electron Microscopy (SEM). Their interaction in the adhesives was identified with SEM, Energy-Dispersive X-ray (EDX), and Micro-Raman spectroscopy. Teeth were sectioned, divided in study groups, and assessed for μTBS and failure mode. Employing Fourier Transform-Infrared (FTIR) spectroscopy, the DC of the adhesives was assessed. EDX mapping revealed the occurrence of oxygen, calcium, and phosphorus in the HAA-5% and HAA-10% groups. HAA-5% had the greatest μTBS values followed by HAA-10%. The presence of apatite was shown by FTIR spectra and Micro-Raman demonstrated phosphate and carbonate groups for n-HA spheres. The highest DC was observed for the CEA group followed by HAA-5%. n-HA spheres exhibited dentin interaction and formed a hybrid layer with resin tags. HAA-5% demonstrated superior μTBS compared with HAA-10% and control adhesive. The DC for HAA-5% was comparable to control adhesive.
Zhurkov’s Stress-Driven Fracture as a Driving Force of the Microcrystalline Cellulose Formation
Authors: Stovbun Sergey V. , Mikhaleva Mariya G. , Skoblin Aleksey A. , Usachev Sergey V. , Nikolsky Sergey N. , Kharitonov Vasily A. , Kovaleva Kseniya I. , Politenkova Galina G. , Vedenkin Alexander S. , Zlenko Dmitry V.
Published: December 2020

Microcrystalline cellulose (MCC) is a chemically pure product of cellulose mechano-chemical conversion. It is a white powder composed of the short fragments of the plant cells widely used in the modern food industry and pharmaceutics. The acid hydrolysis of the bleached lignin-free cellulose raw is the main and necessary stage of MCC production. For this reason, the acid hydrolysis is generally accepted to be the driving force of the fragmentation of the initial cellulose fibers into MCC particles. However, the low sensibility of the MCC properties to repeating the hydrolysis forces doubting this point of view. The sharp, cleave-looking edges of the MCC particles suggesting the initial cellulose fibers were fractured; hence the hydrolysis made them brittle. Zhurkov showed that mechanical stress decreases the activation energy of the polymer fracture, which correlates with the elevated enthalpy of the MCC thermal destruction compared to the initial cellulose.
Characterization of Electrical Heating Performance of CFDM 3D-Printed Graphene/Polylactic Acid (PLA) Horseshoe Pattern with Different 3D Printing Directions
Authors: Kim Hyelim , Lee Sunhee
Published: December 2020

This study manufactured a horseshoe pattern (HP)-type electrical heating element based on a graphene/polylactic acid (GR/PLA) filament using CFDM (conveyor-fused deposition modeling) 3D printing technology, which is a new manufacturing process technology. CFDM 3D printing HP was fabricated in the different printing directions of 0°, 45°, and 90°. To confirm the effects of different 3D printing directions, the morphology, surface resistivity, and electrical heating properties of the different HPs were analyzed. In addition, the CFDM 3D-printed HPs made using different printing directions were printed on cotton fabric to confirm their applicability as fabric heating elements, and their electrical heating properties were measured. Regarding the morphology of the GR/PLA-HP, each sample was stacked according to the printing direction. It was also confirmed through FE-SEM images that the graphene was arranged according to the printing direction in which the nozzle moved. In the XRD pattern analysis, the GR/PLA-HP samples showed two diffraction peaks of PLA and graphene. The sizes of those peaks were increased in the order of 90° < 45° ≤ 0° according to the printing direction, which also affected the electrical and electric heating properties. The surface resistivities of the GR/PLA-HP samples were shown to be increased in the order of 0° < 45° < 90°, indicating that the electrical properties of GR/PLA HP printed at 0° were improved compared to those of the other samples. When 30 V was applied to three GR/PLA-HP samples according to the printing direction, the surface temperatures were decreased in the order of 0° < 45° < 90°, and the samples were indicated as 83.6, 80.6, and 52.5 °C, respectively; the same result was shown when the samples were printed on cotton fabric. Therefore, it was confirmed that the GR/PLA CFDM 3D-printed HP sample printed at 0° direction showed low surface resistivity and high surface temperature, so that improving the electrical heating properties.
Adhesion and Energy Characteristics of Rigid-Chain Polymer Surface: Polyamidoimides
Authors: Chalykh Anatoly E. , Stepanenko Valentina Yu. , Aliev Ali D.
Published: December 2020

The adhesion characteristics and surface energies of two series of polyamidoimides (PAI) with different molecular weights, monomer unit structures, hinge groups in the main chain of the macromolecules, and thermal prehistory were determined via delamination at 180° and test fluids contact angles. We found that PAI are high-energy polymers, the surface energy of which varies in the range from 32 to 45 mJ/m2. In contrast to flexible-chain polymers, the exponent in the McLeod equation is two, which is due to the flat parallel orientation of the macromolecular chains in the surface layers. The main contribution to the change in surface characteristics of these polymers is the change in the packing density of PAI macromolecules, which is reflected mainly in the change in the polymers’ dispersion component. We found that the adhesion properties of PAI with respect to high- and low-energy substrates are determined mainly by the macromolecules packing density in the surface layers with their conformation state unchanged.
Renewable Energy Sources in Transport on the Example of Methyl Esters and Bioethanol
Authors: Kupczyk Adam , Mączyńska-Sęczek Joanna , Golisz Ewa , Borowski Piotr F.
Published: December 2020

The aim of this article is to show how the situation of domestic biofuels in Poland and the alternative fuels sector is changing. On the basis of our own research and the available literature, changes in the production potential of Poland in the field of alternative fuels/biofuels are examined. Moreover, the reasons for changes in the production structure are analyzed. The flexibility of production potential to changes in legal conditions is assessed, and the value of sectors and their future are determined. This paper discusses legal and market aspects related to selected renewable energy sources used in Polish transport. Based on a review of literature and statistical data, the production and use of methyl esters and bioethanol are characterized in detail. Analysis of legal regulations enables the presentation of targets regarding renewable energy source (RES) use in transport by 2030. The results of studies conducted in 2020 are discussed with regard to the value (attractiveness) of methyl esters, bioethanol, electromobility and liquid bio-hydrocarbons, and these are compared and contrasted with the result from earlier years since 2010.
Modeling and Design of the Automatic Pressure Regulating Valve in the Foam Firefighting System
Authors: Yang Zhijun , Chen Hongjun , He Lifang , Wang Xikun
Published: December 2020

This paper presents a novel design of the automatic pressure balancing valve, used in the in-line balanced pressure (ILBP) proportioner for the foam firefighting system, at a required percentage of solution. Featured in a four-chamber configuration with a double-acting diaphragm actuator, it can automatically maintain the foam concentrate pressure with the pressure in the supply water pipeline, within a precision level of 0.02 MPa (or 1.3%), under the design operating condition. The static characteristics at the equilibrium state have been discussed in terms of poppet displacement with reference to the geometrical dimensions and operating pressures of the valve. The dynamic response of the valve during the startup has been examined through building the mathematical model of the forces on the valve and solving it numerically using MATLAB. The results show that the response time of the valve is always less than 0.01 s, which fully satisfies the stability and hysteresis requirement. The prototype has been tested in the laboratory, which agrees well with the numerical results. It was then successfully put into production, forming the first series of ILBP foam pump firefighting system in China.
Improved Statistical Pattern Analysis Monitoring for Complex Multivariate Processes Using Empirical Likelihood
Authors: Shao Jianwen , Zhang Xin , Chen Wenhua , Shen Xiaomin
Published: December 2020

This article developed an improved statistical pattern analysis (SPA) monitoring strategy for fault detection of complex multivariate processes using empirical likelihood. The technique based on statistical pattern analysis performs fault detection by inspecting change in the statistics of process variables (e.g., mean value, correlation coefficient, variance, kurtosis, etc.). It is capable of monitoring non-Gaussian or even nonlinear processes. However, the original SPA framework explicitly computes all the high-order statistics, which significantly increases the scale and dimensionality of the problem, especially in the case of complex multivariate processes. To alleviate this difficulty, we propose monitoring changes in the statistics with the same order using empirical likelihood, which is a widely used estimation method to construct confidence limits or regions for parameters with similar properties. As a result, changes in statistics of the same order can be translated into a single index; hence more information on the faulty conditions can be observed. Furthermore, by considering statistics of the same order, the scale of the problem is reduced significantly. The improved statistical pattern analysis monitoring strategy is suitable for monitoring complex multivariate processes. The performance of the improved method is illustrated by an application study to fault detection of the Tennessee Eastman (TE) process.
Antimicrobial Resistance of Lactobacillus johnsonii and Lactobacillus zeae in Raw Milk
Authors: Výrostková Jana , Regecová Ivana , Kováčová Mariana , Marcinčák Slavomír , Dudriková Eva , Maľová Jana
Published: December 2020

Lactobacillus johnsonii and Lactobacillus zeae are among the lactobacilli with probiotic properties, which occur in sour milk products, cheeses, and to a lesser extent in raw milk. Recently, resistant strains have been detected in various species of lactobacilli. The aim of the study was to determine the incidence of resistant Lactobacillus johnsonii and Lactobacillus zeae strains in various types of raw milk. A total of 245 isolates were identified by matrix-assisted laser desorption/ionization mass spectrometry and polymerase chain reaction methods as Lactobacillus sp., of which 23 isolates of Lactobacillus johnsonii and 18 isolates of Lactobacillus zeae were confirmed. Determination of susceptibility to selected antibiotics was performed using the E-test and broth dilution method, where 7.3% of lactobacilli strains were evaluated as ampicillin-resistant, 14.7% of isolates as erythromycin-resistant, and 4.9% of isolates as clindamycin-resistant. The genus Lactobacillus johnsonii had the highest resistance to erythromycin (34.8%), similar to Lactobacillus zeae (33.3%). Of the 41 isolates, the presence of the gene was confirmed in five Lactobacillus johnsonii strains and in two strains of Lactobacillus zeae. The presence of resistant strains of Lactobacillus johnsonii and Lactobacillus zeae is a potential risk in terms of spreading antimicrobial resistance through the food chain.
Biochemical Composition and Phycoerythrin Extraction from Red Microalgae: A Comparative Study Using Green Extraction Technologies
Authors: Ardiles Paula , Cerezal-Mezquita Pedro , Salinas-Fuentes Francisca , Órdenes Daniel , Renato Gabriel , Ruiz-Domínguez Mari Carmen
Published: December 2020

Porphyridium spp. is a debated family that produces phycoerythrin (PE) for use in multiple industrial applications. We compared the differences in the biochemical composition and phycoerythrin yield of P. cruentum and P. purpureum by conventional and green extraction technologies. The protein content in P. cruentum was 42.90 ±1.84% w/w. The omega-3 fatty acid (FA) was highlighted by eicosapentaenoic acid (EPA, C20:5, ω-3, ~9.74 ± 0.27% FA) and arachidonic acid (ARA, C20:4, ω-6, ~18.02 ± 0.81% FA) represented the major omega-6 fatty acid. Conversely, P. purpureum demonstrated a higher lipid content (17.34 ± 1.35% w/w) and an FA profile more saturated in palmitic (C16:0, 29.01 ± 0.94% FA) and stearic acids (C18:0, 50.02 ± 1.72% FA). Maceration and freeze/thaw were the conventional methods, whereas microwave (MW) and ultrasound (US) served as green procedures for PE extraction under the factorial-design methodology. Aqueous solvents, extraction-time and power were the main factors in the statistical extraction designs based on Response-Surface Methodology (RSM). Overall, the PE extraction yield was higher (2-to 6-fold) in P. cruentum than in P. purpureum. Moreover, green technologies (US > MW) improved the PE recovery in comparison with the conventional methods for both of the microalgae. The maximum PE yield (33.85 mg/g) was obtained under optimal US conditions (15 min and buffer solvent (PBS)) for P. cruentum. Finally, we proved the biochemical differences between the red microalgae and ratified the advantages of using green extraction for PE because it reduced the processing times and costs and increased the economic and functional-applications of bioactive compounds in the industry.
Detecting Vegetation Change in the Pearl River Delta Region Based on Time Series Segmentation and Residual Trend Analysis (TSS-RESTREND) and MODIS NDVI
Authors: Ruan Zhu , Kuang Yaoqiu , He Yeyu , Zhen Wei , Ding Song
Published: December 2020

Time Series Segmentation and Residual Trend analysis (TSS-RESTREND) can detect an abrupt change that was undetected by Residual Trend analysis (RESTREND), but it is usually combined with the Global Inventory for Mapping and Modeling Studies (GIMMS) Normalized Difference Vegetation Index (NDVI), which cannot detect detailed vegetation changes in small areas. Hence, we used Time Series Segmentation and Residual Trend analysis (TSS-RESTREND) and Moderate Resolution Imaging Spectroradiometer (MODIS) NDVI (MOD-TR) to analyze the vegetation dynamic of the Pearl River Delta region (PRD) in this study. To choose the most suitable MODIS NDVI from MOD13Q1 (250 m), MOD13A1 (500 m), and MOD13A2 (1 km), whole and local comparison of results of the break year and MOD-TR were used. Meanwhile, a comparison of vegetation change at the city-scale was also implemented. Moreover, to reduce insignificant trend pixels in TSS-RESTREND, a combination method of TSS-RESTREND and RESTREND (CTSS-RESTREND) was proposed. We found that: (1) MOD13Q1 and MOD13A1 two NDVI were suitable for combination with TSS-RESTREND to detect vegetation change in PRD, but MOD13Q1 was a better choice when considering the accuracy of local detailed vegetation change; (2) CTSS-RESTREND could detect more pixels with a significant change (i.e., significant increase and significant decrease) than those of TSS-RESTREND and RESTREND. Also, its effectiveness could be verified by Landsat data; (3) at the city-scale, the CTSS-RESTREND detected that only vegetation decreases in Shenzhen, Foshan, Dongguan, and Zhongshan were higher than vegetation increases, but, significant vegetation changes (i.e., decreases and increases) were mainly concentrated in Huizhou, Jiangmen, Zhaoqing, and Guangzhou.
Correction: Xu, M., et al. A Modified Geometrical Optical Model of Row Crops Considering Multiple Scattering Frame. Remote Sensing 2020, 12, 3600
Authors: Ma Xu , Liu Yong
Published: December 2020

The authors wish to make the following correction to this paper [...]
A Neural Network-Based Sustainable Data Dissemination through Public Transportation for Smart Cities
Authors: Munjal Rashmi , Liu William , Li Xue Jun , Gutierrez Jairo
Published: December 2020

In recent years, there has been a big data revolution in smart cities dues to multiple disciplines such as smart healthcare, smart transportation, and smart community. However, most services in these areas of smart cities have become data-driven, thus generating big data that require sharing, storing, processing, and analysis, which ultimately consumes massive amounts of energy. The accumulation process of these data from different areas of a smart city is a challenging issue. Therefore, researchers have started aiming at the Internet of vehicles (IoV), in which smart vehicles are equipped with computing and storage capabilities to communicate with surrounding infrastructure. In this paper, we propose a subcategory of IoV as the Internet of buses (IoB), where public buses enable a service as a data carrier in a smart city by introducing a neural network-based sustainable data dissemination system (NESUDA), where opportunistic sensing comprises delay-tolerant data collection, processing and disseminating from one place to another place around the city. The objective was to use public transport to carry data from one place to another and to reduce the traffic from traditional networks and energy consumption. An advanced neural network (NN) algorithm was applied to locate the realistic arrival time of public buses for data allocation. We used the Auckland transport (AT) buses data set from the transport agency to validate our model for the level of accuracy in predicted bus arrival time and scheduled arrival time to disseminate data using bus services. Data were uploaded onto buses as per their dwelling time at each stop and terminals within the coverage area of deployed RSU. The offloading capacity of our proposed data dissemination system showed that it could be utilized to effectively complement traditional data networks. Moreover, the maximum offloading capacity at each parent stop could reach up to 360 GB with a huge saving of energy consumption.
Cloud-BIM Enabled Cyber-Physical Data and Service Platforms for Building Component Reuse
Authors: Xing Ke , Kim Ki Pyung , Ness David
Published: December 2020

While the Circular Economy in the built environment is often viewed in terms of recycling, more value can be obtained from buildings and physical components by their reuse, aided by stewardship and remanufacture, to ensure optimum performance capability. The use of cyber-physical information for online identification, examination and exchange of reusable components may improve their life-cycle management and circularity. To this end, a bi-directional data exchange system is established between physical building components and their virtual Building Information Modeling (BIM) counterparts, so that their life-cycle information—including history of ownership, maintenance record, technical specifications and physical condition—can be tracked, monitored and managed. The resultant prototype Cloud-based BIM platform is then adapted to support an ongoing product-service relationship between suppliers/providers and users/clients. A case study from a major new hospital, focusing upon an example of internal framed glazed systems, is presented for ”proof of concept” and to demonstrate the application of the proposed method. The result of the case study shows that, informed by the life-cycle data from the Cloud-BIM platform, a “lease with reuse” service option is able to deliver a lower total cost and less carbon intensity for each unit of frame-glazed module. This leads to a higher level of eco-efficiency, coupled with decreased consumption of material resources and reduced generation of waste. The research is expected to serve as a step forward in the era of Industry 4.0 and illuminate a more sophisticated way to manage building assets.
An Acceptance Approach for Novel Technologies in Car Insurance
Authors: Milanović Nemanja , Milosavljević Miloš , Benković Slađana , Starčević Dušan , Spasenić Željko
Published: December 2020

Background: Unlike other financial services, technology-driven changes in the insurance industry have not been a vastly explored topic in scholarly literature. Incumbent insurance companies have hitherto been holding their positions using the complexity of the product, heavy regulation, and gigantic balance sheets as paramount factors for a relatively slow digitalization and technological transformation. However, new technologies such as car telematic devices have been creating a new insurance ecosystem. The aim of this study is to assess the telematics technology acceptance for insurance purposes. Methods: The study is based on the Unified Theory of Acceptance and Use of Technology (UTAUT). By interviewing 502 new car buyers, we tested the factors that affect the potential usage of telematic devices for insurance purposes. Results: The results indicate that facilitating conditions are the main predictor of telematics use. Moreover, privacy concerns related to the potential abuse of driving behavior data play an important role in technology acceptance. Conclusions: Although novel insurance technologies are mainly presented as user-driven, users (drivers and insurance buyers) are often neglected as an active party in the development of such technologies.
Is Twitter Indicating a Change in MP’s Views on Climate Change?
Authors: Ebrey Rhian , Hall Stephen , Willis Rebecca
Published: December 2020

Following the release of the Intergovernmental Panel on Climate Change (IPCC) 1.5 °C Special Report in October 2018, there has been a surge in public concern about climate change and demands for greater government action. We analyse the discourse of Members of Parliament (MPs) on climate change on Twitter to examine the extent to which these recent public climate-related events have influenced political agenda-setting. We argue that these events have had two, linked, effects: increased political discourse on climate change, and an increasing use of ‘urgent’ climate language. However, the language style used between political parties differs. Additionally, while the youth strikes and Greta Thunberg, who initiated these strikes, appear to have the greatest influence on MPs’ discourse, the overall relative impact is low, with responses predominately from left- and centrist-political parties. This indicates a clear difference between parties. However, Twitter may not be a suitable platform for investigating Conservative discourse. Further work to explore agenda-setting on Conservative policymaking is required.