bluuebunny/crossref_metadata_2025 huggingface dataset:
Psychometric properties of the Patient Health Questionnaire-4 among Hong Kong young adults in 2021: Associations with meaning in life and suicidal ideation
Authors: Fong Ted C. T. , Ho Rainbow T. H. , Yip Paul S. F.
Published: March 2023

Background

Young adults in Hong Kong are subject to elevated psychological distress given the societal stressors such as civil unrest and COVID-19 pandemic and suicide is a leading cause of death among them. The present study aimed to evaluate the psychometric properties and measurement invariance of the 4-item Patient Health Questionnaire-4 (PHQ-4) as a brief measure of psychological distress and its associations with meaning in life and suicidal ideation (SI) in young adults.

Materials and methods

A mobile survey recruited a large and random sample of 1,472 young adults (Mean age = 26.3 years, 51.8% males) in Hong Kong in 2021. The participants completed the PHQ-4 and Meaning in Life Questionnaire–short form (MLQ-SF) for presence of meaning in life (MIL), SI, COVID-19 impact, and exposure to suicide. Confirmatory factor analysis was conducted to examine the factorial validity, reliability, and measurement invariance of PHQ-4 and MLQ-SF across gender, age, and distress subgroups. Multigroup structural equation model evaluated and compared the direct and indirect effects of latent MIL factor on SI via latent PHQ-4 factor across distress groups.

Results

Both MIL and PHQ-4 supported a 1-factor model with good composite reliability (Ω = 0.80–0.86) and strong factor loadings (λ = 0.65–0.88). Both factors showed scalar invariance across gender, age, and distress groups. MIL showed significant and negative indirect effects (αβ = −0.196, 95% CI = −0.254 to −0.144) on SI via PHQ-4. PHQ-4 showed a stronger mediating role between MIL and SI in the distress group (Δ = −0.146, 95% CI = −0.252 to −0.049) than the non-distress group. Higher MIL predicted higher likelihoods of help-seeking (Odds ratios = 1.46, 95% CI = 1.14–1.88).

Conclusion

The present results support adequate psychometric properties in terms of factorial validity, reliability, convergent validity, and measurement invariance for the PHQ-4 in young adults in Hong Kong. The PHQ-4 demonstrated a substantial mediating role in the relationship between meaning in life and SI in the distress group. These findings support clinical relevance for using the PHQ-4 as a brief and valid measure of psychological distress in the Chinese context.
Ketamine study: Protocol for naturalistic prospective multicenter study on subcutaneous ketamine infusion in depressed patients with active suicidal ideation
Authors: Anzolin Ana Paula , Goularte Jeferson Ferraz , Pinto Jairo Vinícius , Belmonte-de-Abreu Paulo , Cruz Luciane Nascimento , Cordova Victor Hugo Schaly , Magalhaes Lucas Sueti , Rosa Adriane R. , Cereser Keila Maria , Kauer-Sant’Anna Márcia
Published: March 2023

Background

Psychiatric disorders are associated with more than 90% of reported suicide attempts worldwide, but few treatments have demonstrated a direct effect in reducing suicide risk. Ketamine, originally an anesthetic, has been shown anti-suicide effects in clinical trials designed to treat depression. However, changes at the biochemical level were assessed only in protocols of ketamine with very limited sample sizes, particularly when the subcutaneous route was considered. In addition, the inflammatory changes associated with ketamine effects and their correlation with response to treatment, dose-effect, and suicide risk warrant further investigation. Therefore, we aimed to assess whether ketamine results in better control of suicidal ideation and/or behavior in patients with depressive episodes and whether ketamine affects psychopathology and inflammatory biomarkers.

Materials and methods

We report here the design of a naturalistic prospective multicenter study protocol of ketamine in depressive episodes carried out at Hospital de Clínicas de Porto Alegre (HCPA) and Hospital Moinhos de Vento (HMV). The study was planned to recruit adult patients with Major depressive disorder (MDD) or Bipolar disorder (BD) types 1 or 2, who are currently in a depressive episode and show symptoms of suicidal ideation and/or behavior according to the Columbia-Suicide Severity Rating Scale (C-SSRS) and have been prescribed ketamine by their assistant psychiatrist. Patients receive ketamine subcutaneously (SC) twice a week for 1 month, but the frequency can be changed or the dose decreased according to the assistant physician’s decision. After the last ketamine session, patients are followed-up via telephone once a month for up to 6 months. The data will be analyzed using repeated measures statistics to evaluate the reduction in suicide risk as a primary outcome, as per C-SSRS.

Discussion

We discuss the need for studies with longer follow-ups designed to measure a direct impact on suicide risk and that additional information about the safety and tolerability of ketamine in particular subset of patients such as those with depression and ideation suicide. In line, the mechanism behind the immunomodulatory effects of ketamine is still poorly understood.

Trial registration

https://clinicaltrials.gov/, identifier NCT05249309.
Assessing the construct validity of a theory of mind battery adapted to Tunisian school-aged children
Authors: Salhi Imène Soumaya , Lancelot Céline , Marzouki Yousri , Souissi Wided , Besbes Aya Nejiba , Le Gall Didier , Bellaj Tarek
Published: March 2023

Background

Theory of mind (ToM) refers to the ability to understand others’ states of mind, desires, emotions, beliefs, and intentions to predict the content of their mental representations. Two major dimensions within ToM have been studied. The first is the type of inferred mental state, which can be cognitive or affective. The second comprises the types of processes involved according to their degree of complexity (first- and second-order false belief and advanced ToM). ToM acquisition is fundamental—a key component in the development of everyday human social interactions. ToM deficits have been reported in various neurodevelopmental disorders through various tools assessing disparate facets of social cognition. Nevertheless, Tunisian practitioners and researchers lack a linguistically and culturally appropriate psychometric tool for ToM assessment among school-aged children.

Objective

To assess the construct validity of a translated and adapted French ToM Battery for Arabic-speaking Tunisian school-aged children.

Methods

The focal ToM Battery was designed with neuropsychological and neurodevelopmental theory and composed of 10 subtests distributed evenly in three parts: Pre-conceptual, cognitive, and affective ToM. Translated and adapted to the Tunisian sociocultural context, this ToM battery was individually administered to 179 neurotypical Tunisian children (90 girls and 89 boys) aged 7–12 years.

Results

After controlling for the age effect, construct validity was empirically confirmed on two dimensions (cognitive and affective) via structural equation modeling (SEM) analysis, demonstrating that this solution has a good fit. The results confirmed that the age affected differentially the performance obtained on ToM tasks based on the two components of the battery.

Conclusion

Our findings confirm that the Tunisian version of the ToM Battery has robust construct validity for the assessment of cognitive and affective ToM in Tunisian school-aged children; hence, it could be adopted in clinical and research settings.
Association between psychological capital and depressive symptoms during COVID-19: The mediating role of perceived social support and the moderating effect of employment pressure
Authors: Huang Yalian , Lin Xin , Yang Jian , Bai Hefei , Tang Ping , Yuan Guangzhe Frank
Published: March 2023

Introduction

The Coronavirus Disease 2019 (COVID-19) pandemic affects individuals' mental health that can result in fear of getting COVID-19 infection and depression. Prior research has demonstrated that both psychological capital and perceived social support are related to the severity of depression. Yet no study explored the direction of associations between these factors. This undermines the validity of psychological capital as a basis for health interventions.

Methods

This study aimed to explore the association between psychological capital, perceived social support, employment pressure, and depressive symptoms during COVID-19. A cross-sectional design was employed in a sample of 708 Chinese senior medical students who were asked to complete an online questionnaire survey.

Results

Results indicated that psychological capital negatively predicts depressive symptoms (β = −0.55, p < 0.001); perceived social support plays a mediating role in the impact of psychological capital on depressive symptoms (indirect = −0.11, SE = 0.02, p < 0.001, 95%CI [−0.16, −0.07]), and these associations were moderated by employment pressure. Medical students with high employment pressure, the negative impact of psychological capital on depressive symptoms was statistically significant (β = −0.37, SE = 0.05, p < 0.001, 95% CI [−0.046, −0.27]); when the perceived employment pressure was low, the negative effect of psychological capital on depressive symptoms, although significant, was stronger (β = −0.49, SE = 0.04, p < 0.001, 95% CI [−0.57, −0.40]).

Discussion

The current study highlights that it is of great significance to address Chinese medical students' employment pressure and improve their mental health during the COVID-19 epidemic.
Age- and sex-dependent increase in self-harm among adolescents with mental health problems in East China during COVID-19 related society-wide isolation
Authors: Liu Wenjing , Hu Zhishan , Liu Zhen , Zhang Fang , Ding Yue , Shui Ying , Yang Zhi , Cheng Wenhong
Published: March 2023

Objective

The COVID-19 pandemic has raised concerns about child and adolescent mental health issues, such as self-harm. The impact of society-wide isolation on self-harming behaviors among adolescents in China is unclear. In addition, adolescents of different ages and sexes have varying abilities to cope with environmental changes. However, these differences are rarely considered in self-harm studies. We aimed to characterize the age- and sex-dependent effects of COVID-19-related society-wide isolation on self-harm among adolescents in East China.

Methods

We collected 63,877 medical records of children and adolescents aged 8–18 who had an initial visit to Shanghai Mental Health Center in China between 2017 and 2021 and charted annual self-harm rates for each age and sex. Using interrupted time series analysis, we modeled global and seasonal trends and the effect of COVID-19-related society-wide isolation on self-harm rates.

Results

Females aged 10–17 and males aged 13–16 exhibited significantly increasing trends in self-harm rate (pfdr < 0.05) in the past 5 years. Eleven-year-old females in 2020 showed a self-harm rate (37.30%) that exceeded the peak among all ages in 2019 (age 13, 36.38%). The COVID-19-related society-wide isolation elevated self-harm rates in female patients aged 12 [RR 1.45 (95% CI 1.19–1.77); pfdr = 0.0031] and 13 years [RR 1.33 (95% CI 1.15–1.5); pfdr = 0.0031], while males were less affected. Further, females with emotional disorders dominated the increased self-harm rates.

Conclusion

Society-wide isolation has had a significant impact on early adolescent females in East China, especially for those with emotional disturbances, and has brought forward the peak in adolescent self-harm rates. This study calls for attention to the risk of self-harm in early adolescents.
Effects of a lifestyle intervention on the biomarkers of oxidative stress in non-communicable diseases: A systematic review
Authors: Husain Sarah , Hillmann Katharina , Hengst Karin , Englert Heike
Published: March 2023

Oxidative stress plays a critical role in the pathogenesis of chronic diseases. Therefore, improvement of oxidative stress status through lifestyle intervention can play a vital role in preventing and treating chronic diseases. This systematic review aims to provide an overview of articles published in the last decade examining the association between lifestyle intervention and oxidative stress biomarkers in the context of non-communicable diseases. The electronic databases PubMed and Web of Science were searched for relevant studies, following the PRISMA (Preferred Reporting of Systematic Reviews and Meta-Analyses) guidelines. This systematic review focused on the four important oxidative stress biomarkers; glutathione (GSH), superoxide dismutase (SOD), catalase, and malondialdehyde. 671 articles were identified, of which nine met the inclusion criteria. A trend emerged, showing that lifestyle modifications that focus on diet and physical health can improve oxidative stress in the form of an increase in superoxide dismutase and CAT levels and a decrease in Malondialdehyde levels in participants with non-communicable diseases (NCDs), GSH levels were not affected. However, the results are difficult to compare because of the heterogeneity of the methods of the biomarkers studied. Our review indicates that oxidative stress can be influenced by lifestyle modifications and may be an effective tool for the prevention and management of non-communicable diseases. This review also elucidated the importance of analyzing multiple oxidative stress biomarkers to evaluate oxidative stress, it further highlights the need to conduct long-term lifestyle intervention studies on oxidative stress biomarkers to understand the connection between oxidative stress biomarkers, NCDs and Lifestyle intervention.
“We are at war”: The military rhetoric of COVID-19 in cross-cultural perspective of discourses
Authors: Giorgis Paola , Semenets Olena , Todorova Bilyana
Published: March 2023

At the outburst of the COVID-19 pandemic and all throughout its continuation in 2020 and 2021, the metaphor of ‘war' has been one of the most pervasive and recurrent globally. As an international, cross-cultural group of scholars and practitioners, we will analyze critically the communicative strategies enacted and the political agenda that they have meant to serve in Italy, Bulgaria, and Ukraine discussing both the cultural differences and the cross-cultural similarities of such a discourse that has been shaping the perception of our factual reality during the pandemic. Expressions like ‘We are at war', ‘Our heroes are fighting at the forefront', ‘We will win this war' and the like contributed to create symbolical cross-cultural responses that, by playing on emotions such as fear, uncertainty and, in some cases, national pride, contributed to the creation of a new state of reality, that of the “new normality”, calling for specific actions and behaviors. However, the war metaphor assumed different hues according to the country in which it was disseminated, up to the actual appointment of generals as governmental spoke-persons or organizers of the vaccine logistics, often combined with the construction and the mediatization of the archetypical hero fighting against the virus/enemy. To analyze how, all throughout 2020 and 2021, the military rhetoric was implemented and disseminated as the dominant discourse, we draw on Media Representations of the Real, on Rhetoric Studies on Manipulation, on Political Discourse, on Critical Discourse Studies, and on Susan Sontag's fundamental essay Illness as Metaphor. We discuss such rhetorical strategies as they originated from a discussion within our collective project in other words, an online dictionary that, besides critically analyzing contextualized keywords that (re)produce different forms of Otherness, offers creative proposals to reverse such narratives, and can be used as a free resource in different social and educational contexts (www.iowdictionary.org).
Policy implications for gaps in traditional plastic waste material flow analysis: Palmerston North, New Zealand
Authors: Farrelly Trisia , Chitaka Takunda Yeukai
Published: March 2023

The Basel Plastic Waste Amendments reflect growing global concern about the illegal plastic waste trade as waste colonialism. Comprehensive analyses of plastic waste material sources, pathways, and fates are needed for effective plastic waste trade policy. Plastics waste flows from Palmerston North, New Zealand to Malaysia highlight potential gaps in plastic waste trade policies. The authors recommend strengthening New Zealand's national waste policy framework and the Basel Convention's Plastics Amendments by basing policy responses on critical transboundary plastic waste material flow analyses, establishing harmonized definitions including “recyclable” and “environmentally sound recycling”; regulating contamination thresholds and container inspections; and waste trade traceability, transparency, compliance, enforcement, and remediation; reclassifying fluorinated polymers and thermosets as “hazardous”; and prioritizing principles of prevention, proximity and precaution over future investments in the management of plastic waste.
“Breast is best”… until they say so
Authors: Quinones Cristina
Published: March 2023

In this autoethnographic article, I discuss the consequences of being exposed to two competing breastfeeding discourses during my first mothering experience—the “self-regulated dyad” and the “externally regulated dyad” discourse. The former represents the ideal scenario and the evidence-based practices recommended by the World Health Organization (i.e., breastfeeding on demand, internally regulated by the dyad). The externally regulated discourse refers to the standardized health interventions that take over when difficulties arise (e.g., weight gain deviations and latching issues). Building on Kugelmann's critique about our blind reliance on “standardized health,” existing evidence, and my breastfeeding journey, I argue that unqualified and unindividualized breastfeeding interventions are highly counterproductive. To illustrate these points, I discuss the implications of the polarized interpretation of pain and the limited dyadically focused support. I then move on to analyze how ambivalent social positioning around breastfeeding impacts our experience. In particular, I found that I was highly regarded as a “good, responsible mum” up till my baby was 6 months, and how breastfeeding became increasingly challenged by others when my daughter was approaching her first birthday. Here, I discuss how performing attachment mothering identity work allowed me to navigate these challenges. Against this backdrop, I reflect upon feminist ambivalent positionings on breastfeeding and the complexity of balancing the promotion of women's hard-earned rights while supporting them to engage in whatever baby-feeding choice they feel appropriate. I conclude that unless we acknowledge the physical and social complexities of the process, and our healthcare systems seriously invest in allocating human resources and training them appropriately, breastfeeding rates may continue to suffer and women continue to interiorize it as their own failure.
Energetic proton acceleration by EMIC waves in Io’s footprint tail
Authors: Clark G. , Szalay J. R. , Sulaiman A. H. , Saur J. , Kollmann P. , Mauk B. H. , Paranicas C. , Hue V. , Greathouse T. , Allegrini F. , Glocer A. , Garcia-Sage K. , Bolton S.
Published: March 2023

In this study, we present a survey of energetic proton observations associated with Io’s footprint tail (FPT) and compare their signatures with in situ measurements of the plasma waves and lower-energy electron environments. We find further supporting evidence that proton acceleration in Io’s FPT is likely a consequence of wave–particle interactions via electromagnetic ion cyclotron waves that are generated by precipitating electrons into Jupiter’s ionosphere. This idea was originally proposed by Clark et al. (2020) and Sulaiman et al. (2020) based on NASA’s Juno mission likely transiting Io’s Main Alfvén Wing (MAW) during its twelfth orbit (i.e., PJ12). Additionally, the analysis of > 50 keV protons presented here highlights important observational details about the Io–Jupiter interaction as follows: 1) proton acceleration in Io’s FPT is a persistent feature and the energy flux carried by the protons is highest at smaller Io-Alfvén tail distances; 2) energetic protons exhibit positive correlations with both plasma waves and <100 keV/Q electrons; 3) during a small number of Io FPT crossings, the protons display finer spatial/temporal structure reminiscent of the electron observations reported by Szalay et al. (2018); and 4) the proton pitch angle distributions are characterized by two types: conic distributions in or near Io’s MAW and isotropic elsewhere.
Assessing the diet quality, environmental impact, and monetary costs of the dietary transition in China (1997–2011): Impact of urbanization
Authors: Chang Zhiyao , Cai Hongyi , Talsma Elise F. , Fan Shenggen , Ni Yuanying , Wen Xin , Veer Pieter van't , Biesbroek Sander
Published: March 2023

Background

Increased urbanization has been linked to transitions in dietary patterns. However, evidence on the impacts of urbanization on diet quality, and environmental impact, and diet cost is limited. The aim of this study was to investigate the time trends of these three dietary sustainability in China over the period 1997–2011 and to examine their associations with urbanization.

Methods

Food consumption of 8,330 participants (18–64y) of the China Health and Nutrition Survey cohort (1997, 2000, 2004, 2006, 2009, and 2011) were examined and diet quality was assessed using the Chinese Healthy Eating Index 2016 (CHEI2016). Dietary related environmental impacts on Greenhouse Gas Emissions (GHGE), Total Water Use (TWU), and Land Use (LU) were estimated using the Chinese Food Life Cycle Assessment Database. Monetary cost of diet was calculated using the community market prices of food items. Multilevel mixed-effects models were used to estimate associations between the time trend of dietary sustainability indicators and degree of urbanization.

Results

From 1997 to 2011, the CHEI2016 score increased by 10.6%, GHGE by 23.8%, LU by 29.1%, and the inflation-corrected cost of diet by 80%. Urbanization was positively associated with these time trends, which remained after adjustment for sociodemographic and lifestyle factors (all P < 0·05).

Conclusion

The rapid urbanization in China over the past two decades has been followed by an improvement in the overall dietary quality, but this has been accompanied by an increase in the environmental impacts and higher cost of the diet, especially in communities with lower urbanization index.
Combining ability estimates for quality and non-quality protein maize inbred lines for grain yield, agronomic, and quality traits
Authors: Amegbor Isaac Kodzo , van Biljon Angeline , Shargie Nemera Geleta , Tarekegne Amsal , Labuschagne Maryke T.
Published: March 2023

Introduction

Biofortified maize varieties could contribute to the fight against hunger and malnutrition of the increasing human population and help meet the high demand of maize for human consumption, industrial use and feed for animal and poultry. The understanding of the genetic mechanisms conditioning the inheritance of grain yield and other agronomic and quality traits is essential in the development of superior maize genotypes. The main objective of this study was to determine the combining ability for grain yield and other agronomic traits of QPM and non-QPM inbred lines crossed with two QPM and two non-QPM testers.

Materials and methods

A total of 130 hybrids were obtained by crossing 10 non-QPM and 23 QPM lines with four elite testers (two QPM and two non-QPM). The 130 single cross hybrids were evaluated at 13 sites in 2018 and 2019.

Results and discussion

The results showed significant general combining ability (GCA) for lines and testers as well as significant specific combining ability (SCA) for hybrids for most of the measured traits. Non-additive gene action controlled the inheritance of grain yield while agronomic and quality traits were controlled by additive gene action. QPM lines 11, 14 and 28 and non-QPM tester CML444 showed desirable GCA effects for grain yield, indicating that these lines in combination with tester CML444 should be considered when targeting development of superior maize genotypes with QPM traits.

Conclusions

Based on the SCA values, crosses 120, 108, 105, 99, 85, and 41 were identified as the best hybrids across the locations. It is recommended that maize breeding programs targeting the development of high yielding QPM hybrids should exploit QPM lines with high GCA values for heterosis to be realized. In addition, the identified superior hybrids may be further evaluated and consequently promoted for commercial release which could increase incomes of farmers and help to alleviate poverty, hunger and malnutrition in southern Africa and sub-Saharan Africa at large.
Ultrasound-guided endoscopy to improve accuracy of persistent urogenital sinus (PUGS) diagnosis in adult patient: A case report
Authors: Mirandola Mariateresa , Gui Benedetta , Naldini Angelica , Foschi Nazario , Casula Viola , Testa Antonia Carla , Scambia Giovanni , Catena Ursula
Published: March 2023

Introduction

persistent urogenital sinus (PUGS) is a rare condition characterized by abnormal communication between the urethra and vagina, that can frequently be associated with other complex Mullerian malformation (33%). We present a case of PUGS associated with a complex Mullerian malformation diagnosed in adult age after the integration of gynecological ultrasound with hysteroscopy, both performed by expert operators.

Case description

27-year-old women was referred to our clinic because of frequent urinary tract infections and cyclic pelvic pain. She was virgo, with normal menstrual cycles and dysmenorrhea. A didelphys uterus and double vagina with bilateral hematocolpos was firstly diagnosed through transrectal and transabdominal ultrasound. An MRI was then performed and a monorenal and ipsilateral ureteral malformation were diagnosed; in addition, a complete absence of the lower third of the vagina and an abnormal origin of the urethra from the bladder were described. Patient underwent lower genital tract endoscopy: external vaginal orifice was obliterated, a PUGS was diagnosed and both vaginas' ostia were detected on the PUGS's posterior-lateral walls. The procedure was performed under transabdominal ultrasound guidance which confirmed the endoscopic anatomical suspicion, avoiding complications such as perforation and misdiagnosis.

Discussion

ultrasound guided endoscopy plays an essential role in the evaluation of complex anatomic anomalies, such as persistent urogenital sinus (PUGS), leading to a dynamic one-stop diagnosis; it avoids delays and misdiagnosis in preoperative assessment possibly related to the separately application of different radiological and endoscopic techniques.
Molecular Cloning, Screening of Single Nucleotide Polymorphisms, and Analysis of Growth-Associated Traits of igf2 in Spotted Sea Bass (Lateolabrax maculatus)
Authors: Fan Sigang , Wang Pengfei , Zhao Chao , Yan Lulu , Zhang Bo , Qiu Lihua
Published: March 2023

The insulin-like growth factor 2 gene (igf2) is thought to be a key factor that could regulate animal growth. In fish, few researchers have reported on the single nucleotide polymorphisms (SNPs) located in igf2 and their association with growth traits. We screened the SNPs of igf2 from the spotted sea bass (Lateolabrax maculatus) by Sanger sequencing and made an association between these SNPs with growth traits. The full-length complementary (c) DNA of igf2 was 1045 bp, including an open reading frame of 648 bp. The amino acid sequence of Igf2 contained a signal peptide, an IGF domain, and an IGF2_C domain. Multiple sequence alignment showed that the IGF domain and IGF2_C domain were conserved in vertebrates. The genome sequence of igf2 had a length of 6227 bp. Fourteen SNPs (13 in the introns and one in one of the exons) were found in the genome sequence of igf2. Four SNPs located in the intron were significantly associated with growth traits (p < 0.05). These results demonstrated that these SNPs could be candidate molecular markers for breeding programs in L. maculatus.
Isolation of Potential Probiotic Bacillus spp. from the Intestine of Nile Tilapia to Construct Recombinant Probiotic Expressing CC Chemokine and Its Effectiveness on Innate Immune Responses in Nile Tilapia
Authors: Nakharuthai Chatsirin , Boonanuntanasarn Surintorn , Kaewda Jirawadee , Manassila Pimpisut
Published: March 2023

This study aimed to investigate the potential probiotic Bacillus spp. from the intestine of Nile tilapia in order to construct a recombinant probiotic for the enhancement of the Nile tilapia immune response. One hundred bacterial isolates from the intestine of Nile tilapia were characterized for species identification using the 16s ribosomal RNA (rRNA). Only Bacillus isolates with exhibited antagonistic activity were investigated for their biological functions, which included protease-producing capacity, bile salts and pH tolerance, antibiotic susceptibility, and pathogenicity tests. According to the best results, Bacillus isolate B29, as closely related to B. subtilis, was selected to construct a recombinant probiotic for the delivery of CC chemokine protein (pBESOn-CC). The existence of recombinant probiotics was confirmed by Western blotting before the feeding trial. In addition, the CC chemokine mRNA level was quantified in the intestine of fish fed probiotics after 30 days of feeding. Total immunoglobulin, lysozyme activity, alternative complement 50 activity (ACH50), and phagocytic activity of fish fed either wild-type or recombinant probiotics were significantly increased, indicating that probiotics could stimulate the Nile tilapia immune system through different processes. Interestingly, the dietary supplementation of recombinant probiotics has a stronger immune response enhancement than the wild-type strain.
Influence of an Automatic Enrichment Device on Laying Hen Behavior and Plumage Condition
Authors: Riedel Anna , Canci Meryem , Spindler Birgit , Kemper Nicole
Published: March 2023

Feather pecking and cannibalism are prominent problems in modern laying hen husbandry. Among the various approaches to address this issue, environmental enrichment plays a crucial role. In this on-farm study, four winter gardens of an organic farm henhouse were equipped with an automatic enrichment device. Different quantities of downpipes dosing grain on rough-coated pecking plates (PPs) were tested. One group served as a control (CG) without an automatic enrichment device, while the others were offered different numbers of PPs, with one equipped with a doubled amount of PPs (DEG) compared to the other two groups (SEG). Video analyses of the hens’ duration of stay and behaviors in the recorded winter garden area and around the PPs were performed, and regular assessments of the plumage condition were conducted. By the end of the production cycle, no hens with intact plumage were found, with hens in the CG and DEG showing worse scores and earlier deterioration in the plumage condition than in the SEG. The offer of PPs showed a significant influence on the duration of stay in the filmed area. Hens in the DEG stayed significantly longer (mean: 129 s, SD: 126 s) than those in the CG (mean: 79 s, SD: 91 s; p < 0.05) and SEG (mean: 75 s, SD: 83 s; p < 0.005). On the performed behavioral bouts per hen and minute (CG mean bouts/minute (SD): 5.47 (2.92); SEG mean (SD): 5.33 (2.76); SEG mean (SD): 5.81 (3.24)), no significant influences were detected. Environmental pecking was the behavior most frequently observed in all winter gardens, where, particularly around the PPs, pecking at the device was observed. Therefore, the enrichment device can be assessed as well accepted by the hens in winter gardens. The effect of the device on the plumage condition remains unclear, with external factors probably showing a greater influence than the enrichment.
Fluorescence Microscopy and Flow-Cytometry Assessment of Substructures in European Red Deer Epididymal Spermatozoa after Cryopreservation
Authors: Dziekońska Anna , Lecewicz Marek , Partyka Agnieszka , Niżański Wojciech
Published: March 2023

Thawed spermatozoa, sampled post mortem from the fresh epididymides of European red deer and epididymides stored for up to 12 h at 2–4 °C, were evaluated by fluorescence microscopy (FM) and flow cytometry (FC). The sperm samples were extended and cryopreserved. The sperm motility (CASA), sperm viability (SYBR+/PI-), acrosome integrity, mitochondrial activity, apoptotic changes, and chromatin stability were assessed. Sperm were analyzed by FM before cryopreservation, and by FM and FC after thawing. Epididymal storage time (for 12 h) had no significant effect (p > 0.05) on the examined variables before cryopreservation. After thawing, the storage variants differed (p ˂ 0.05) in the percentage of apoptotic sperm (FM and FC) and DNA integrity (FC). The results of FM and FC differed (p ˂ 0.05) in all the analyzed parameters, excluding SYBR+/PI. Significant correlations (p ˂ 0.01) were observed between the sperm viability, acrosome integrity, and the percentage of non-apoptotic spermatozoa, regardless of the applied technique. In FM, the above parameters were also significantly correlated with mitochondrial activity. The study demonstrated that European red deer spermatozoa stored in the epididymides at 2–4 °C for 12 h can be used for cryopreservation. Both techniques were equally reliable, but FM was better suited for evaluating mitochondrial activity whereas FC was more useful in the evaluation of DNA fragmentation.
Reproductive Performance of Tunisian Arabian Stallions: A Study on the Variance and Estimation of Heritability
Authors: Jlassi Mariem , Jemmali Bayrem , Ouzari Hadda Imen , Lasfer Faten , Aoun Belgacem Ben , Ben Gara Abderrahmane
Published: March 2023

A consistently high level of stallion fertility plays an economically important role in modern horse breeding. To better understand the factors affecting variation in stallion fertility, we have performed a statistical analysis study on some breeding factors: year of breeding, stud farm, age of the stallion, number of covered mares per stallion, reproduction methods, and age of the mare. This work was conducted on 94 purebred Arabian stallions in four different regions of Tunisia. The results showed an increase in the number of stallions during the study period, ranging from 11.33% in 2011 to 13.29% in 2018. Sidi Thabet’s stud farm contained the highest number of purebred Arabian stallions. The majority of stallions were between 15 and 21 years old and had covered 1 to 20 mares; 95.19% of stallions were used in natural mating (Nat); 50.36% had low fertility, 17.69% had medium fertility, and 32.3% had excellent fertility according to fertility standards. Depending on the year and stud, there was a variation in fertility per cycle (FERPCE) and end-of-season fertility (FERPSE) of the stallions. The highest average FERPCE and FERPSE values were obtained using artificial insemination with fresh semen (AIF). Analysis of FERPCE and FERPSE showed that the model used in our study explained 40.21% of total variability observations for FERPCE and 42.1% for FERPSE. The used statistical model showed that the breeding year, the stud, the age of the stallion, the number of covered mares by stallions and the method of reproduction significantly affected both FERPCE and FERPSE (with p = 0.001). Low to moderate heritability estimations for FERPCE (hs2 = 0.08) and FERPSE (hes2 = 0.36) were obtained.
Identification of Candidate Genes and Functional Pathways Associated with Body Size Traits in Chinese Holstein Cattle Based on GWAS Analysis
Authors: Abdalla Ismail Mohamed , Hui Jiang , Nazar Mudasir , Arbab Abdelaziz Adam Idriss , Xu Tianle , Abdu Shaima Mohamed Nasr , Mao Yongjiang , Yang Zhangping , Lu Xubin
Published: March 2023

Body size is one of the most economically important traits of dairy cattle, as it is significantly associated with cow longevity, production, health, fertility, and environmental adaptation. The identification and application of genetic variants using a novel genetic approach, such as genome-wide association studies (GWASs), may give more insights into the genetic architecture of complex traits. The identification of genes, single nucleotide polymorphisms (SNPs), and pathways associated with the body size traits may offer a contribution to genomic selection and long-term planning for selection in dairy cows. In this study, we performed GWAS analysis to identify the genetic markers and genes associated with four body size traits (body height, body depth, chest width, and angularity) in 1000 Chinese Holstein cows. We performed SNPs genotyping in 1000 individuals, based on the GeneSeek Genomic Profiler Bovine 100 K. In total, we identified 11 significant SNPs in association with body size traits at the threshold of Bonferroni correction (5.90 × 10−7) using the fixed and random model circulating probability unification (FarmCPU) model. Several genes within 200 kb distances (upstream or downstream) of the significant SNPs were identified as candidate genes, including MYH15, KHDRBS3, AIP, DCC, SQOR, and UBAP1L. Moreover, genes within 200 kb of the identified SNPs were significantly enriched (p ≤ 0.05) in 25 Gene Ontology terms and five Kyoto Encyclopedia of Genes and Genomes pathways. We anticipate that these results provide a foundation for understanding the genetic architecture of body size traits. They will also contribute to breeding programs and genomic selection work on Chinese Holstein cattle.
A Scientometric Study to a Critical Review on Promising Anticancer and Neuroprotective Compounds: Citrus Flavonoids
Authors: Qiu Mingyang , Wei Wenlong , Zhang Jianqing , Wang Hanze , Bai Yuxin , Guo De-an
Published: March 2023

Flavonoids derived from citrus plants are favored by phytomedicinal researchers due to their wide range of biological activities, and relevant studies have been sustained for 67 years (since the first paper published in 1955). In terms of a scientometric and critical review, the scientometrics of related papers, chemical structures, and pharmacological action of citrus flavonoids were comprehensively summarized. The modern pharmacological effects of citrus flavonoids are primarily focused on their anticancer activities (such as breast cancer, gastric cancer, lung cancer, and liver cancer), neuroprotective effects (such as anti-Alzheimer’s disease, Parkinson’s disease), and metabolic diseases. Furthermore, the therapeutic mechanism of cancers (including inducing apoptosis, inhibiting cell proliferation, and inhibiting cancer metastasis), neuroprotective effects (including antioxidant and anti-inflammatory), and metabolic diseases (such as non-alcoholic fatty liver disease, type 2 diabetes mellitus) were summarized and discussed. We anticipate that this review could provide an essential reference for anti-cancer and neuroprotective research of citrus flavonoids and provide researchers with a comprehensive understanding of citrus flavonoids.
Research on Recognition and Analysis of Teacher–Student Behavior Based on a Blended Synchronous Classroom
Authors: Xu Taojie , Deng Wei , Zhang Si , Wei Yantao , Liu Qingtang
Published: March 2023

Due to the impact of the COVID-19 pandemic, many students are unable to attend face-to-face courses, Therefore, in this case, distance education should be promoted to replace face-to-face education. However, because of the imbalance of education in different regions, such as the imbalance of education resources between rural and urban areas, the quality of distance education may not be guaranteed. Therefore, in China and some regions, there have been efforts made to carry out blended synchronous classroom attempts. In hybrid synchronous classroom situations, teachers’ workloads have increased, and it is difficult to fully understand students’ learning efficiency and class participation. We use deep learning to identify the behaviors of teachers and students in a blended synchronous classroom-based situation, aiming to automate the analysis of classroom videos, which can help teachers in classroom reflection and summary in a blended synchronous classroom or face-to-face classroom. In the behavior recognition of students and teachers, we combine the head, hand, and body posture information of teachers and students and add the feature pyramid (FPN) and convolutional block attention module (CBAM) for comparative experiments. Finally, S–T (student–teacher) analysis and engagement analysis were carried out on the identification results.
Extraction and Analysis of Soil Salinization Information in an Alar Reclamation Area Based on Spectral Index Modeling
Authors: Hong Guojun , Bai Tiecheng , Wang Xingpeng , Li Mingzhe , Liu Chengcheng , Cong Lianjie , Qu Xinyi , Li Xu
Published: March 2023

In order to explore the optimal remote sensing salinity monitoring index model for the inversion of soil salinization in the Alar reclamation area, based on the Sentinel-2 images and field measured data, the salinity index 1 (SI1), the normalized difference vegetation index in a green–red band (GRNDVI), the normalized vegetation index of greenness (GNDVI), and the normalized difference vegetation index (NDVI) were selected to construct the remote sensing-based salinization 1 detection index (S1DI) model. Next, the cotton field soil salinization information in the Alar reclamation area was extracted, and the accuracy of the model was verified to obtain the optimal remote sensing salinity monitoring index model. The results show that the overall classification accuracy of the S1DI1 (SI1-GRNDVI), S1DI2 (SI1-GNDVI), S1DI3 (SI1-NDVI), and S1DI4 (SI1-DVI) models for salinity monitoring is 83.35%, 83.10%, 82.96%, and 80.25%, respectively. The S1DI1 model is most suitable for retrieving the degree of soil salinization in the cotton field in the Alar reclamation area, and the S1DI2, S1DI3, and S1DI4 models are also very useful for monitoring soil salinization in the Alar reclamation area. Using the S1DI1 model to invert the soil salinization level of the cotton fields in the Alar reclamation area, it was found that the cotton field in the reclamation area is dominated by non-saline soil, and the light saline soil and moderate saline soil are mainly distributed in the 9th and 12th clusters of the reclamation area. As the S1DI1 model possesses the highest accuracy in extracting the soil salinization information of the cotton fields in the Alar reclamation area, it can be used as a remote sensing salinity 1 monitoring index model for the inversion of the soil salinization of the cotton fields in the reclamation area, which is expected to provide an effective reference value for soil salinization monitoring.
Hygrothermal Behavior of Cultural Heritage Buildings and Climate Change: Status and Main Challenges
Authors: Dias Pereira Luisa , Saraiva Nuno Baía , Soares Nelson
Published: March 2023

This paper follows a bibliometric assessment methodology to provide a systematic review of studies devoted to cultural heritage (CH) buildings that simultaneously refer to hygrothermal conditions and/or behavior and climate change (CC) scenarios. Moreover, this paper aims at identifying the main research trend and research gaps in this combined research field, and to provide a critical discussion of previous works relating to CH, hygrothermal behavior, and CC. Scopus database was used for data gathering and export, followed by VOSviewer for network visualization. The advanced search on the database showed that only 36 documents have considered the study of these three subjects together, as of 2022. The majority of research has been carried out in Italy (27% of the relevant documents). This paper further describes some funded research projects on the topic of CH, hygrothermal behavior, and CC. It was concluded that the investigation on this combined research topic is very recent and scarcely unexplored, and further research is required to assess how future CC scenarios will affect the hygrothermal behavior of CH buildings. These new understandings are fundamental for all stakeholders involved in topics related to heritage conservation and assessment of the hygrothermal behavior of buildings.
The Travel Time of Floatable Litter of Different Densities Influenced by River Flow Velocity
Authors: Nabilah Adzhar Nur Fatin , Abd Manaf Latifah , Azmar Noor Azwani , Nadhirah Roslan Aimi , Bagheri Milad , Yusuf Kpalo Sunday , Asma Wan Talaat Wan Izatul , Beiranvand Pour Amin
Published: March 2023

Rivers are a source of life. However, these past years, the sustainability of rivers around the world has been threatened by urbanization, industrialization, and rapid development. These activities put pressure on waterway systems and rivers, leading to the emergence of riverine litter. The lack of understanding of the migration of litter in rivers can affect the decision-making efficiency of policymakers when it comes to riverine litter management. Therefore, the primary objective of this study is to evaluate the travel time for different densities of Floatable Litter (FL) in rivers influenced by river flow velocity. The density of the selected FL was manually determined by assessing litter characteristics of mass, volume, and size. The Sg. Berkelah river in Pahang, Malaysia was used as the simulation medium for the sampling of FL travel time utilizing the extrapolation of the Time of Travel (ToT) approach. The ToT technique utilizes a 30 m distance. The sampling was done under three different river flow velocity conditions. In this study, a correlation analysis between these two variables was carried out and evaluated. The results revealed that magazine papers possess the fastest FL travel time, with T = 43.93 s when v = 0.230 m/s, while cloth possesses the slowest FL travel time, with T = 204.90 s when v = 0.167 m/s. The travel time of FL is not influenced by low-class density, p < 1000 kg/m3, but by other factors that have a stronger influence on the travel time of horizontally migrated litter. This study provides a basic understanding and overview of FL migrating characteristics in rivers for further reference by local authorities for litter monitoring and also future riverine litter studies.
Leveraging Adversarial Samples for Enhanced Classification of Malicious and Evasive PDF Files
Authors: Trad Fouad , Hussein Ali , Chehab Ali
Published: March 2023

The Portable Document Format (PDF) is considered one of the most popular formats due to its flexibility and portability across platforms. Although people have used machine learning techniques to detect malware in PDF files, the problem with these models is their weak resistance against evasion attacks, which constitutes a major security threat. The goal of this study is to introduce three machine learning-based systems that enhance malware detection in the presence of evasion attacks by substantially relying on evasive data to train malware and evasion detection models. To evaluate the robustness of the proposed systems, we used two testing datasets, a real dataset containing around 100,000 PDF samples and an evasive dataset containing 500,000 samples that we generated. We compared the results of the proposed systems to a baseline model that was not adversarially trained. When tested against the evasive dataset, the proposed systems provided an increase of around 80% in the f1-score compared to the baseline. This proves the value of the proposed approaches towards the ability to deal with evasive attacks.