High‐resolution 7 tesla postmortem MRI for quantitative analysis of structure‐pathology correlations in neurodegenerative diseases
Authors: Khandelwal Pulkit , Duong Michael Tran , Sadaghiani Shokufeh , Lim Sydney A , Denning Amanda E , Chung Eunice , Ravikumar Sadhana , Ravikumar Sadhana , Arezoumandan Sanaz , Peterson Claire , Bedard Madigan , Capp Noah , Ittyerah Ranjit , Migdal Elyse , Choi Grace , Kopp Emily , Patino Bridget Loja , Hasan Eusha , Li Jiacheng , Bahena Alejandra , Prabhakaran Karthik , Mizsei Gabor , Gabrielyan Marianna , Schuck Theresa , Trotman Winifred , Robinson John L. , Ohm Daniel T , Lee Eddie B , Trojanowski John Q , McMillan Corey T , Grossman Murray , Irwin David J , Detre John A. , Tisdall Dylan M , Das Sandhitsu R. , Wisse Laura E.M. , Wolk David A , Yushkevich Paul A. 
Published: January 2025

Background

Postmortem MRI allows brain anatomy to be examined at high‐resolution linking pathology with morphometric measurements. However, automated methods for analyzing postmortem MRI are not well developed. We present a deep learning‐based framework for automated segmentation of cortical mantle, subcortical structures (caudate, putamen, globus pallidus, and thalamus), white matter hyperintensities (WMH), and normal appearing white matter in (n=135) postmortem human brain tissue specimens (Table 1) imaged at 0.3 mm3 T2w 7T spanning Alzheimer’s disease and related dementias. We show generalizing capabilities across unseen images acquired at 0.28 mm3 and 0.16 mm3 T2*w 7T FLASH sequence. We report associations between localized cortical thickness and volumetric measurements across key regions and semi‐quantitative neuropathological ratings.

Method

A deep learning model was trained on manually segmented images to produce automated whole‐brain hemisphere segmentations (Figure 1) with a post‐hoc topological correction step to delineate buried sulcus. We report regional patterns of association between localized cortical thickness at 16 anatomical locations and neuropathology ratings of regional measures of p‐tau, neuronal loss; global amyloid‐β, Braak staging, and CERAD ratings obtained from histology data in a subset (n=82) with AD continuum diagnoses. We correlate subcortical volumetry and regional cortical thickness with WMH burden (Figure 2) for the entire cohort (n=135). All analyses include age, sex, and postmortem interval as covariates.

Result

Tau pathology in Braak regions play an important role in cortical atrophy and cognitive decline in AD. Significant negative correlations (Figure 2) between p‐tau and cortical thickness were found in angular gyrus and midfrontal regions. Cortical thickness showed significant negative correlation with neuronal loss in Brodmann area (BA) 35 and entorhinal cortex (ERC), and with Braak staging in midfrontal, ERC and BA35, regions consistent with high p‐tau uptake in PET imaging with cortical thickness on MRI. High WMH volume disrupts structural and functional connectivity impacting memory. Significant negative correlation of WMH volume with thickness in posterior cingulate and superior temporal regions was observed.

Conclusion

Our automated postmortem MRI framework provides geometrically accurate segmentations of several key brain regions. Our analysis linking morphometry and pathology measurements demonstrated that automated segmentation and analysis of postmortem MRI can complement and inform antemortem neuroimaging studies.