bluuebunny/crossref_metadata_2025 huggingface dataset:
Genome-Wide Identification of GATA Family Genes in Potato and Characterization of StGATA12 in Response to Salinity and Osmotic Stress
Authors: Zhu Xi , Duan Huimin , Zhang Ning , Majeed Yasir , Jin Hui , Li Wei , Chen Zhuo , Chen Shu , Tang Jinghua , Zhang Yu , Si Huaijun
Published: November 2024

GATA factors are evolutionarily conserved transcription regulators that are implicated in the regulation of physiological changes under abiotic stress. Unfortunately, there are few studies investigating the potential role of GATA genes in potato plants responding to salt and osmotic stresses. The physicochemical properties, chromosomal distribution, gene duplication, evolutionary relationships and classification, conserved motifs, gene structure, interspecific collinearity relationship, and cis-regulatory elements were analyzed. Potato plants were treated with NaCl and PEG to induce salinity and osmotic stress responses. qRT-PCR was carried out to characterize the expression pattern of StGATA family genes in potato plants subjected to salinity and osmotic stress. StGATA12 loss-of-function and gain-of-function plants were established. Morphological phenotypes and growth were indicated. Photosynthetic gas exchange was suggested by the net photosynthetic rate, transpiration rate, and stomatal conductance. Physiological indicators and the corresponding genes were indicated by enzyme activity and mRNA expression of genes encoding CAT, SOD, POD, and P5CS, and contents of H2O2, MDA, and proline. The expression patterns of StGATA family genes were altered in response to salinity and osmotic stress. StGATA12 protein is located in the nucleus. StGATA12 is involved in the regulation of potato plant growth in response to salinity and osmotic stress. Overexpression of StGATA12 promoted photosynthesis, transpiration, and stomatal conductance under salinity and osmotic stress. StGATA12 overexpression induced biochemical responses of potato plants to salinity and osmotic stress by regulating the levels of H2O2, MDA, and proline and the activity of CAT, SOD, and POD. StGATA12 overexpression induced the up-regulation of StCAT, StSOD, StPOD, and StP5CS against salinity and osmotic stress. StGATA12 could reinforce the ability of potato plants to resist salinity and osmosis-induced damages, which may provide an effective strategy to engineer potato plants for better adaptability to adverse salinity and osmotic conditions.
Impact of Circadian Clock PER2 Gene Overexpression on Rumen Epithelial Cell Dynamics and VFA Transport Protein Expression
Authors: Ali Rahmat , Zhen Yongkang , Zanna Xi , Lin Jiaqi , Zhang Chong , Ma Jianjun , Zhong Yuhong , Husien Hosameldeen Mohamed , Saleh Ahmad A. , Wang Mengzhi
Published: November 2024

The circadian gene PER2 is recognized for its regulatory effects on cell proliferation and lipid metabolism across various non-ruminant cells. This study investigates the influence of PER2 gene overexpression on goat rumen epithelial cells using a constructed pcDNA3.1-PER2 plasmid, assessing its impact on circadian gene expression, cell proliferation, and mRNA levels of short-chain fatty acid (SCFA) transporters, alongside genes related to lipid metabolism, cell proliferation, and apoptosis. Rumen epithelial cells were obtained every four hours from healthy dairy goats (n = 3; aged 1.5 years; average weight 45.34 ± 4.28 kg), cultured for 48 h in vitro, and segregated into control (pcDNA3.1) and overexpressed (pcDNA3.1-PER2) groups, each with four biological replicates. The study examined the potential connection between circadian rhythms and nutrient assimilation in ruminant, including cell proliferation, apoptosis, cell cycle dynamics, and antioxidant activity and the expression of circadian-related genes, VFA transporter genes and regulatory factors. The introduction of the pcDNA3.1-PER2 plasmid drastically elevated PER2 expression levels by 3471.48-fold compared to controls (p < 0.01), confirming effective overexpression. PER2 overexpression resulted in a significant increase in apoptosis rates (p < 0.05) and a notable reduction in cell proliferation at 24 and 48 h post-transfection (p < 0.05), illustrating an inhibitory effect on rumen epithelial cell growth. PER2 elevation significantly boosted the expression of CCND1, WEE1, p21, and p16 (p < 0.05) while diminishing CDK4 expression (p < 0.05). While the general expression of intracellular inflammation genes remained stable, TNF-α expression notably increased. Antioxidant marker levels (SOD, MDA, GSH-Px, CAT, and T-AOC) exhibited no significant change, suggesting no oxidative damage due to PER2 overexpression. Furthermore, PER2 overexpression significantly downregulated AE2, NHE1, MCT1, and MCT4 mRNA expressions while upregulating PAT1 and VH+ ATPase. These results suggest that PER2 overexpression impairs cell proliferation, enhances apoptosis, and modulates VFA transporter-related factors in the rumen epithelium. This study implies that the PER2 gene may regulate VFA absorption through modulation of VFA transporters in rumen epithelial cells, necessitating further research into its specific regulatory mechanisms.
Age of Cafeteria Diet Onset Influences Obesity Phenotype in Mice in a Sex-Specific Manner
Authors: Bazhan Nadezhda , Kazantseva Antonyna , Dubinina Anastasia , Balybina Natalia , Jakovleva Tatiana , Makarova Elena
Published: November 2024

We investigated the influence of sex and the age of obesogenic diet initiation on the obesity phenotypes at a later age. C57Bl mice started the Cafeteria Diet (CafD, with increased fat and carbohydrates, ad libitum, from 7 weeks of age (7CafD, pre-puberty) or 17 weeks of age (7CafD, post-puberty) while control C57Bl mice were fed regular chow. At 27 weeks of age, 7CafD males (n = 9) compared to 17CafD males (n = 7) had lower body weight, white adipose tissue (WAT) relative weight, and plasma cholesterol levels, and a higher expression of thermogenic genes in WAT and brown adipose tissue (BAT), and fatty acid oxidation (FAO) and insulin signalling genes in muscles. The 7CafD females (n = 8), compared to 17CafD females (n = 6), had higher plasma triglyceride levels and hepatic glycogen content, but lower insulin sensitivity and hepatic expression of FAO and insulin signalling genes. The 7CafD females, compared to 7CafD males, had more WAT, and a reduced expression of FAO genes in muscles and thermogenic genes in WAT. The 17CafD females, compared to 17CafD males, had lower plasma leptin and insulin levels, and higher insulin sensitivity and expression of insulin signalling genes in the liver and muscles. Thus, the initiation of the obesogenic diet before puberty led to a more adaptive metabolic phenotypes in males, and after puberty, in females.
Tlx Promotes Stroke-Induced Neurogenesis and Neuronal Repair in Young and Aged Mice
Authors: Khan Dilaware , Bock Dagmar , Liu Hai-Kun , Muhammad Sajjad
Published: November 2024

Stroke is one of the leading causes of chronic disability in humans. It has been proposed that the endogenous neural stem/progenitor cells generate new neurons in the damaged area. Still, the contribution of these cells is negligible because a low number of newborn mature neurons are formed. Tlx conventional knock-out mice, Tlx-CreERT2 mice, and Tlx-overexpressing (Tlx-OE) mice were specifically chosen for their unique genetic characteristics, which were crucial for the experiments. Permanent and transient middle cerebral artery occlusion was used to induce stroke in the mice. Immunostainings for doublecortin and GFP/BrdU/NeuN were performed to study neurogenesis and fate mapping. The rotarod test was performed to assess motor deficits. Here, we show that stroke-induced neurogenesis is dramatically increased with the additional expression of two copies of the nuclear receptor-coding gene tailless (Tlx, also known as Nr2e1), which has been shown to be a master regulator of subventricular zone (SVZ) neural stem cells (NSCs). We show that Tlx expression is upregulated after stroke, and stroke-induced neurogenesis is blocked when Tlx is inactivated. Tlx overexpression in NSCs leads to massive induction of neurogenesis via stroke. More newborn mature neurons are formed in Tlx-overexpressing mice, leading to improved coordination and motor function recovery. Most importantly, we also demonstrate that this process is sustained in aged mice, where stroke-induced neurogenesis is nearly undetectable in wild-type animals. This study provides the first stem cell-specific genetic evidence that endogenous NSCs can be exploited by manipulating their master regulator, Tlx, and thus suggests a novel therapeutic strategy for neuronal repair.
Estimation of Mango Fruit Production Using Image Analysis and Machine Learning Algorithms
Authors: Arcila-Diaz Liliana , Mejia-Cabrera Heber I. , Arcila-Diaz Juan
Published: November 2024

Mango production is fundamental to the agricultural economy, generating income and employment in various communities. Accurate estimation of its production optimizes the planning and logistics of harvesting; traditionally, manual methods are inefficient and prone to errors. Currently, machine learning, by handling large volumes of data, emerges as an innovative solution to enhance the precision of mango production estimation. This study presents an analysis of mango fruit detection using machine learning algorithms, specifically YOLO version 8 and Faster R-CNN. The present study employs a dataset consisting of 212 original images, annotated with a total of 9604 labels, which has been expanded to include 2449 additional images and 116,654 annotations. This significant increase in dataset size notably enhances the robustness and generalization capacity of the model. The YOLO-trained model achieves an accuracy of 96.72%, a recall of 77.4%, and an F1 Score of 86%, compared to the results of Faster R-CNN, which are 98.57%, 63.80%, and 77.46%, respectively. YOLO demonstrates greater efficiency, being faster in training, consuming less memory, and utilizing fewer CPU resources. Furthermore, this study has developed a web application with a user interface that facilitates the uploading of images from mango trees considered samples. The YOLO-trained model detects the fruits of each tree in the representative sample and uses extrapolation techniques to estimate the total number of fruits across the entire population of mango trees.
Glypican-3 and Cytokeratin-19 Expression in Pancreatic Cancer in a Canadian Population
Authors: Bekkers Carley , Ramjeesingh Ravi , Arnason Thomas
Published: November 2024

Background/Objectives: One study of pancreatic ductal adenocarcinoma has found expression of glypican-3 (GPC3) and cytokeratin-19 (CK19) determined by immunohistochemistry to be associated with higher stage and grade disease, with a more adverse prognosis. The reported 44% rate of GPC3 expression in pancreatic cancer raises the important possibility that targeted immunotherapies currently in development for hepatocellular carcinoma may also prove useful for GPC3-expressing pancreatic cancers. The present study aims to determine if a similar expression pattern of these markers and stage/grade/prognostic associations is present in our Canadian patient population. Methods: Patients with a pancreatic surgical resection for adenocarcinoma or neuroendocrine tumor (NET) were identified from pathology records over a 5-year period. Immunohistochemistry for GPC3 and CK19 was performed on archived tumor tissue and the proportion of positive cells and intensity of staining were recorded. Grade, stage, and overall survival were compared in patients with NETs that were CK19-positive versus -negative. Results: All 72 pancreatic adenocarcinomas and 20 NETs tested were negative for GPC3, apart from a single case of pancreatic adenocarcinoma. All 72 adenocarcinomas were positive for CK19 expression. Half of the NETs were positive for CK19. There was no correlation between CK19 expression in NETs and tumor grade, lymph node metastasis, distant metastasis, or overall survival. Conclusions: We are skeptical of the reported prognostic value of GPC3 and CK19 in pancreatic adenocarcinomas. CK19 as a prognostic marker in NETs has potential for further study. The results with our protocol for GPC3 immunohistochemistry suggest that pancreatic cancer may be a less promising target for GPC3-targeted immunotherapies than previously thought.
Quantitative Approach to Quality Review of Prenatal Ultrasound Examinations: Estimated Fetal Weight and Fetal Sex
Authors: Combs C. Andrew , Lee Ryan C. , Lee Sarah Y. , Amara Sushma , Ashimi Balogun Olaide
Published: November 2024

Background/Objectives: Systematic quality review of ultrasound exams is recommended to ensure accurate diagnosis. Our primary objectives were to develop a quantitative method for quality review of estimated fetal weight (EFW) and to assess the accuracy of EFW for an entire practice and for individual personnel. A secondary objective was to evaluate the accuracy of fetal sex determination. Methods: This is a retrospective cohort study. Eligible ultrasound exams included singleton pregnancies with live birth and known birth weight (BW). A published method was used to predict BW from EFW for exams with ultrasound-to-delivery intervals of up to 12 weeks. Mean error and median absolute error (AE) were compared between different personnel. Image audits were performed for exams with AE > 30% and exams with reported fetal sex different than newborn sex. Results: We analyzed 1938 exams from 890 patients. In the last exam before birth, the median AE was 5.9%, and the predicted BW was within ±20% of the actual BW in 97.2% of patients. AE was >30% in 28 exams (1.4%); image audit found correct caliper placement in all 28. Only two patients (0.2%) had AE > 30% on the last exam before birth. One sonographer systematically over-measured head and abdominal circumferences, leading to EFWs that were overestimated. Reported fetal sex differed from newborn sex in seven exams (0.4%) and five patients (0.6%). Images in four of these patients were annotated with the correct fetal sex, but a clerical error was made in the report. In one patient, an unclear image was labeled “probably female”, but the newborn was male. Conclusions: The accuracy of EFW in this practice was similar to literature reports. The quantitative analysis identified a sonographer with outlier measurements. Time-consuming image audits could be focused on a small number of exams with large errors. We suggest some enhancements to ultrasound reporting software that may help to reduce clerical errors. We provide tools to help other practices perform similar quality reviews.
Advancements in 3D Transoesophageal Echocardiography (TOE) and Computed Tomography (CT) for Stroke Prevention in Left Atrial Appendage Occlusion Interventions
Authors: Hajhosseiny Reza , Ariff Ben , Cole Graham , Koa-Wing Michael , Pabari Punam , Sutaria Nilesh , Qureshi Norman , Kanagaratnam Prapa , Rana Bushra
Published: November 2024

Left atrial appendage occlusion (LAAO) has emerged as a highly effective alternative to oral anticoagulation for stroke prevention in patients with non-valvular atrial fibrillation. Precise pre-procedural planning and meticulous post-procedural follow-up are essential for achieving successful LAAO outcomes. This review explores the latest advancements in three-dimensional (3D) transoesophageal echocardiography (TOE) and computed tomography (CT) imaging modalities, which have considerably improved the planning, intra-procedural guidance, and follow-up processes for LAAO interventions. Innovations in 3D TOE and CT imaging have transformed the approach to LAAO by providing a more detailed and accurate assessment of the left atrial appendage, enabling clinicians to acquire comprehensive anatomical and morphological information, crucial for optimising device selection and positioning, thus reducing the risk of complications and enhancing the overall safety and efficacy of the procedure. Post-procedurally, CT and TOE imaging are invaluable in the monitoring of patients, ensuring that the device is correctly positioned and functioning as intended. Early detection of any complications (e.g., device-related thrombus and peri-device leaks) can help to risk-stratify patient at increased risk of stroke and initiate timely interventions, thereby improving long-term outcomes for patients.
Aetiology, Treatment and Outcomes of Pericarditis: Long-Term Data from a Longitudinal Retrospective Single-Centre Cohort
Authors: Giordani Andrea Silvio , Bocaj Iris , Vicenzetto Cristina , Baritussio Anna , Gregori Dario , Scognamiglio Federico , Ocagli Honoria , Marcolongo Renzo , Caforio Alida Linda Patrizia
Published: November 2024

Background. Pericarditis has a heterogeneous clinical spectrum and rate of relapse. Data on aetiology, real-life treatment strategies, and long-term course from contemporary pericarditis cohorts are lacking. Methods. Pericarditis patients referred to the Cardioimmunology Outpatient Clinic at Padua University Hospital in 2001–2020 were retrospectively included. Kaplan–Meier method was used for recurrence-free survival probability estimation. The appropriateness of treatment was assessed based on the European Society of Cardiology guidelines. Results. One-hundred forty-four patients (57% males, mean age 50 years) followed up for 18 months (IQR 7–45) were included; of those, 52% had acute, 35% recurrent, 8% incessant, and 5% chronic pericarditis; 9% had cardiac tamponade at diagnosis. Time to pericardial effusion resolution was 53 days (IQR 16–124); median medical treatment duration was 87 days (IQR 48–148). Treatment was readjusted following the ESC guidelines for nonsteroidal anti-inflammatory drugs in 29% of the cases, steroids in 12%, and colchicine in 25%. Eleven (8%) patients were treated with anti-IL1 agents. Recurrence-free survival probability was 86% at 1st-year follow-up, and 23 patients (16%) had at least one recurrence, with a mean of two relapses per patient. Compared to patients without recurrences, they had a higher frequency of cardiac tamponade (27% vs. 6%, p = 0.006) and left bundle branch block (14% vs. 1%, p = 0.034). Out of the 144 patients, 5 (3%) were diagnosed as having constrictive pericarditis at first evaluation at our clinic, underwent successful pericardiectomy, and are currently alive and asymptomatic. Conclusions. When treated following a guideline-based approach, pericarditis has a favourable evolution. A relevant quote of cases benefits from the treatment readjustment of previously prescribed medical therapy when not in line with ESC recommendations. Cases relapsing despite treatment readjustment should receive anti-IL1 therapies.
Cervical Open-Door Laminoplasty for Myelopathy Caused by Ossification of the Posterior Longitudinal Ligament: Correlation Between Spinal Canal Expansion and Clinical Outcomes
Authors: Ko Young-Il , Kim Young-Hoon , Barraza Jorge , Ko Myung-Sup , Bang Chungwon , Hwang Byung Jun , Kim Sang-Il , Park Hyung-Youl
Published: November 2024

Background/Objectives: This study investigated the relationship between spinal canal expansion and clinical outcomes in patients with myelopathy due to ossification of the posterior longitudinal ligament (OPLL) who underwent cervical open-door laminoplasty. Methods: A retrospective study was conducted on 36 OPLL patients who underwent open-door laminoplasty between 2009 and 2021. Preoperative and two-year postoperative radiologic parameters, including bony canal area (BCA) and spinal canal area (SCA), were measured. Clinical outcomes were assessed using the Numerical Rating Scale (NRS) for neck pain and radicular pain, the Neck Disability Index (NDI), and Japanese Orthopaedic Association (JOA) scores. Results: The mean expansion of BCA was 112.1 mm2 (47%) and SCA was 100.5 mm2 (64%). All clinical outcomes improved after surgery, although not statistically significant. JOA scores improved significantly in the severe group, while NDI and NRS-neck scores improved in the mild to moderate group. Significant correlations were found between improvements in NRS-neck and expansions of BCA (r = 0.533, p = 0.001) and SCA (r = 0.537, p = 0.001). NDI improvement was also associated with BCA expansion. No significant correlations were found between canal expansion and NRS-R, NRS-L, or JOA scores. Conclusions: Cervical open-door laminoplasty effectively increased the bony and spinal canal areas in patients with OPLL and myelopathy. In addition to improving myelopathy symptoms, this procedure may also improve neck pain and disability. Further research is needed to assess the long-term outcomes and to better understand these clinical improvements.
Anti-OX40 Biological Therapies in the Treatment of Atopic Dermatitis: A Comprehensive Review
Authors: Marfil-Cantón Myriam , Prados-Carmona Alvaro , Cebolla-Verdugo Marta , Husein-ElAhmed Husein , Campos Fernando , Ruiz-Villaverde Ricardo
Published: November 2024

Introduction. Atopic dermatitis (AD) is the most prevalent inflammatory dermatological disorder, affecting a significant percentage of the global population. This chronic disease has a multifactorial and intricate pathogenesis, influenced by genetic predisposition, skin barrier dysfunction, immune dysregulation, neuroimmune mechanisms, and alterations in the skin microbiome, among other factors. Methods. The treatment of AD has faced significant clinical challenges due to the ineffectiveness of conventional therapies. However, recent advances in understanding its pathophysiology have led to the introduction of new therapeutic options. Recently, the OX40 receptor has been identified as a key factor in the development of AD. Recent studies have demonstrated that blocking the OX40 ligand with monoclonal antibodies significantly and sustainably improves the signs and symptoms of moderate to severe AD. Results. A comprehensive review of the available literature on anti-OX40 treatments in atopic dermatitis that evaluates their mechanism of action, their clinical efficacy, and the prospects of this promising therapeutic option for improving AD management is provided. Conclusions. Anti-OX40 and anti-OX40L blockers are a promising therapeutic alternative for the management of moderate–severe atopic dermatitis. Prospective analytical studies are needed to determine whether this new therapeutic target represents a qualitative advance in modifying the progression of the disease.
How Eyelid Changes May Impact on Tears
Authors: Di Zazzo Antonio , Villani Edoardo , Barabino Stefano , Giannaccare Giuseppe
Published: November 2024

This article examines the impact of eyelid margin diseases on tear film composition and associated ocular surface disorders. It highlights the prevalence of blepharitis and meibomian gland dysfunction, discussing risk factors and diagnostic considerations. Various therapeutic approaches, including eyelid hygiene, antibiotics, and innovative treatments, are explored. Emphasizing the chronic nature of these conditions, the article underscores the need for patient compliance. Overall, it provides a concise overview of eyelid-related issues and potential management strategies.
The Association Between Chronic Obstructive Pulmonary Disease (COPD) and Migraine: Systematic Review and Meta-Analysis
Authors: Alshehri Saleem , Zain Al-Abeden Maha Saad , Aldukain Mona , Aldukain Ali , Almuteri Dhai , Hobani Assal , Barakat Abdulmalik , Alzoum Nora
Published: November 2024

Background/Objectives: Migraine and chronic obstructive pulmonary disease (COPD) are both common chronic conditions that may share underlying pathophysiological mechanisms despite presenting with distinct clinical features. Understanding the association between these two conditions could enhance treatment strategies and improve patient outcomes. This review aims to evaluate the relationship between COPD and migraine, highlighting shared risk factors and identifying gaps in the existing literature. Methods: A search was conducted across four electronic databases (PubMed, Ovid Medline, ScienceDirect, and Web of Science) following PRISMA guidelines up to January 2024. The search identified 85 studies, of which five met the inclusion criteria: observational studies, cohort studies, case–control studies, and randomized controlled trials examining the association between migraine and COPD. Studies not published in English and irrelevant study designs were excluded. The risk of bias was assessed using the JBI Critical Appraisal Tool, which evaluated aspects such as study design, participant selection, measurement methods, and the handling of confounding factors. Results: The review included five studies comprising 184,817 patients. All studies identified a significant association between COPD and migraine, with varying methodologies for diagnosing the conditions. Notably, COPD patients had an increased risk of migraine compared to controls, and migraine patients also demonstrated a higher risk of developing COPD. However, the evidence was marked by high heterogeneity and potential confounding factors. Conclusions: The findings suggest a significant association between COPD and migraine, potentially driven by shared mechanisms such as systemic inflammation. However, the predominance of cross-sectional studies limits causal inference. Future research should prioritize longitudinal studies to clarify the directionality and causality of the relationship between COPD and migraine while thoroughly addressing potential confounding factors.
Oral Manifestations in Patients in Treatment with Antidepressants: A Systematic Review
Authors: Alcázar-Hernández Juan Manuel , Pecci-Lloret Miguel Ramón , Guerrero-Gironés Julia
Published: November 2024

Background/Objectives: The rising use of antidepressants is linked to oral health risks, including xerostomia, caries, and periodontal disease. Recognizing these risks is essential for improving patient care. To systematically review oral manifestations in patients undergoing antidepressant treatment. Methods: This review follows the PRISMA guidelines and includes observational studies published in the last 21 years. A PICO-based question was developed to select relevant studies, which were assessed for quality using a modified STROBE checklist. Results: A total of 11 studies were analyzed, revealing a consistent association between antidepressant use and the increased risk of xerostomia, caries, and periodontal disease. Additional findings included taste dysfunction and oral bleeding complications. Among the antidepressants, selective serotonin reuptake inhibitors (SSRIs) and tricyclic antidepressants (TCAs) were most commonly associated with xerostomia and caries. However, no significant impact was observed on the chemical composition of saliva or on hemostasis in invasive dental procedures. Conclusions: Antidepressant use may lead to significant oral health issues, notably xerostomia and caries. Further studies are recommended to clarify the influence of specific antidepressants and confounding factors, such as treatment duration, dosage, and hygiene habits, on oral health outcomes.
The Five Pillars of Acute Right Ventricular Heart Failure Therapy: Can We Keep the Pediment in Balance?
Authors: Petriş Antoniu Octavian , Pop Călin , Cimpoeşu Diana Carmen
Published: November 2024

Acute right ventricular heart failure (aRHF), a long-neglected aspect of heart disease, has recently gained attention due to an improved understanding of its pathophysiology and the development of tailored therapeutic strategies. The therapeutic approach is now built on several pillars that aim to support the stable clinical condition of the patient, starting with the central pillar of etiological or specific therapy and extending to various aspects related to hemodynamic support, ventilation support, fluid optimization, and, when necessary, advanced resources such as right ventricular assist devices (e.g., extracorporeal membrane oxygenation—ECMO, Impella RP, or ProtekDuo). This five-pillar approach summarizes the different facets of contemporary treatment for aRHF, although some aspects related to their use are still being clarified.
Greater Tuberosity Healing Rate and Clinical Results Following RSA Are Similar for Two Fracture-Specific Implant Systems
Authors: Rotman Dani , Avraham Omer , Goldstein Yariv , Kazum Efi , Rojas Lievano Jorge , Chechik Ofir , Maman Eran
Published: November 2024

Background: Various fracture-specific reverse shoulder arthroplasty (RSA) systems exist on the market. We set out to examine whether the type of prosthesis used and the means of fixation (cemented or non-cemented) influenced the rate of tuberosity healing or the functional outcome of the operation. Methods: This retrospective cohort multicenter study included 146 patients who underwent RSA for an acute three- or four-part proximal humerus fracture and had a minimum follow-up of one year. Six fellowship-trained surgeons at two different centers performed all operations. The implants were either Tornier Aequalis or Depuy Delta Xtend, both fracture-specific Grammont-style systems. Results: The mean age ± standard deviation (SD) was 76 ± 7 years, and 83% of patients were female. The mean ± SD follow-up time was 30 ± 31 months. The Aequalis prosthesis was used in 82 patients (56%), and the Delta Xtend in 64 patients (44%). A total of 105 RSAs (72%) were cemented. Tuberosity healing rate was similar for the two implant systems (71% Aequalis vs. 82% Delta Xtend, p = 0.15) and for the cemented or non-cemented, respectively (73% cemented vs. 83% non-cemented, p = 0.22). There was no significant difference in the motion and functional outcomes between the two implant systems in this study. Conclusions: RSA for complex PHF in the elderly has similar short-term results, regardless of the type of fracture-specific implant or the fixation technique (cemented vs. cementless).
Cancer and the 10-Year Incidence of Chronic Low Back Pain in 407,314 Adults Followed in General Practices in Germany
Authors: Kostev Karel , Latourte Augustin , Yon Dong Keon , Haro Josep Maria , Richette Pascal , Beaudreuil Johann , Jacob Louis
Published: November 2024

Objective: There is a scarcity of data on the long-term relationship between cancer and chronic low back pain (CLBP). Therefore, this retrospective cohort study investigated the association between cancer and the 10-year incidence of CLBP in Germany. Methods: Data collected in 1293 German general practices between 2005 and 2022 were used for the study. Patients diagnosed with cancer were matched to those without cancer (1:1) using a propensity score based on age, sex, the mean number of consultations per year during the follow-up, index year, and several chronic conditions. The index date was the consultation corresponding to cancer diagnosis in the cancer group and a random visit date in the noncancer group. The analyses included Kaplan–Meier curves with the log-rank test and Cox regression models adjusted for other frequent conditions. Results: There were 203,657 adults in the cancer group and 203,657 adults in the noncancer group. The mean (SD) age was 66.2 (14.6) and 66.0 (13.8) years in patients with and without cancer, respectively, with a proportion of women of 51.3–51.8%. Within 10 years of the index date, 16.1% of people with cancer and 18.8% of those without cancer were diagnosed with CLBP (p-value < 0.001). The Cox regression analysis corroborated this finding, as there was a negative and significant association between cancer and CLBP (HR = 0.87, 95% CI = 0.86–0.89). Conclusions: Cancer was not associated with increased odds of CLBP in the decade following its diagnosis in Germany. Due to limitations inherent to the data, caution should be taken when interpreting the study results.
Blood Pressure Patterns and Hepatosteatosis: Cardiometabolic Risk Assessment in Dipper and Non-Dipper Phenotypes
Authors: Astan Ramazan , Patoulias Dimitrios , Ninić Ana , Dayanan Ramazan , Karakasis Paschalis , Mercantepe Tolga , Mercantepe Filiz , Klisic Aleksandra
Published: November 2024

Background/Objectives: Non-dipper hypertension (HT), a condition in which blood pressure does not drop sufficiently at night compared to daytime, is considered a serious condition that increases the risk of cardiovascular disease, stroke, and organ damage. This study aimed to examine the relationship between dipper and non-dipper blood pressure patterns, hepatosteatosis, and biochemical markers in hypertensive and normotensive individuals. Methods: Demographic, biochemical, and hepatic ultrasonography data from 142 patients who underwent 24 h ambulatory blood pressure measurement (ABPM) were evaluated retrospectively and cross-sectionally in this study. Patients were categorized into four groups based on ABPM results: non-dipper normotensive (NDN), dipper normotensive (DN), non-dipper hypertensive (NDH), and dipper hypertensive (DH). Results: The study results indicate that NDH individuals had markedly elevated levels of hepatosteatosis and uric acid compared with DH and normotensive persons (p < 0.001). The grade of hepatosteatosis showed significant discriminatory capacity in differentiating between dipper and non-dipper hypertensive patients, with an AUC of 0.861, specificity of 94%, and sensitivity of 66%. Individuals with hypertension exhibiting a non-dipper pattern demonstrate a greater prevalence of hepatosteatosis and elevated uric acid levels. Conclusions: The study findings show non-dipper patterns have a higher risk for cardiometabolic diseases. This indicates that not only blood pressure, but also metabolic disorders should be closely monitored and treated in the management of non-dipper HT.
Vessel Traffic Flow Prediction in Port Waterways Based on POA-CNN-BiGRU Model
Authors: Chang Yumiao , Ma Jianwen , Sun Long , Ma Zeqiu , Zhou Yue
Published: November 2024

Vessel traffic flow forecasting in port waterways is critical to improving safety and efficiency of port navigation. Aiming at the stage characteristics of vessel traffic in port waterways in time sequence, which leads to complexity of data in the prediction process and difficulty in adjusting the model parameters, a convolutional neural network (CNN) based on the optimization of the pelican algorithm (POA) and the combination of bi-directional gated recurrent units (BiGRUs) is proposed as a prediction model, and the POA algorithm is used to search for optimized hyper-parameters, and then the iterative optimization of the optimal parameter combinations is input into the best combination of iteratively found parameters, which is input into the CNN-BiGRU model structure for training and prediction. The results indicate that the POA algorithm has better global search capability and faster convergence than other optimization algorithms in the experiment. Meanwhile, the BiGRU model is introduced and compared with the CNN-BiGRU model prediction; the POA-CNN-BiGRU combined model has higher prediction accuracy and stability; the prediction effect is significantly improved; and it can provide more accurate prediction information and cycle characteristics, which can serve as a reference for the planning of ships’ routes in and out of ports and optimizing the management of ships’ organizations.
Detection of Marine Oil Spill from PlanetScope Images Using CNN and Transformer Models
Authors: Kang Jonggu , Yang Chansu , Yi Jonghyuk , Lee Yangwon
Published: November 2024

The contamination of marine ecosystems by oil spills poses a significant threat to the marine environment, necessitating the prompt and effective implementation of measures to mitigate the associated damage. Satellites offer a spatial and temporal advantage over aircraft and unmanned aerial vehicles (UAVs) in oil spill detection due to their wide-area monitoring capabilities. While oil spill detection has traditionally relied on synthetic aperture radar (SAR) images, the combined use of optical satellite sensors alongside SAR can significantly enhance monitoring capabilities, providing improved spatial and temporal coverage. The advent of deep learning methodologies, particularly convolutional neural networks (CNNs) and Transformer models, has generated considerable interest in their potential for oil spill detection. In this study, we conducted a comprehensive and objective comparison to evaluate the suitability of CNN and Transformer models for marine oil spill detection. High-resolution optical satellite images were used to optimize DeepLabV3+, a widely utilized CNN model; Swin-UPerNet, a representative Transformer model; and Mask2Former, which employs a Transformer-based architecture for both encoding and decoding. The results of cross-validation demonstrate a mean Intersection over Union (mIoU) of 0.740, 0.840 and 0.804 for all the models, respectively, indicating their potential for detecting oil spills in the ocean. Additionally, we performed a histogram analysis on the predicted oil spill pixels, which allowed us to classify the types of oil. These findings highlight the considerable promise of the Swin Transformer models for oil spill detection in the context of future marine disaster monitoring.
Automatic Reading and Reporting Weather Information from Surface Fax Charts for Ships Sailing in Actual Northern Pacific and Atlantic Oceans
Authors: Jian Jun , Zhang Yingxiang , Xu Ke , Webster Peter J.
Published: November 2024

This study is aimed to improve the intelligence level, efficiency, and accuracy of ship safety and security systems by contributing to the development of marine weather forecasting. The accurate and prompt recognition of weather fax charts is very important for navigation safety. This study employed many artificial intelligent (AI) methods including a vectorization approach and target recognition algorithm to automatically detect the severe weather information from Japanese and US weather charts. This enabled the expansion of an existing auto-response marine forecasting system’s applications toward north Pacific and Atlantic Oceans, thus enhancing decision-making capabilities and response measures for sailing ships at actual sea. The OpenCV image processing method and YOLOv5s/YOLO8vn algorithm were utilized to make template matches and locate warning symbols and weather reports from surface weather charts. After these improvements, the average accuracy of the model significantly increased from 0.920 to 0.928, and the detection rate of a single image reached a maximum of 1.2 ms. Additionally, OCR technology was applied to retract texts from weather reports and highlighted the marine areas where dense fog and great wind conditions are likely to occur. Finally, the field tests confirmed that this auto and intelligent system could assist the navigator within 2–3 min and thus greatly enhance the navigation safety in specific areas in the sailing routes with minor text-based communication costs.
Typhoon Storm Surge Simulation Study Based on Reconstructed ERA5 Wind Fields—A Case Study of Typhoon “Muifa”, the 12th Typhoon of 2022
Authors: Zhang Xu , Zuo Changsheng , Wang Zhizu , Tao Chengchen , Han Yaoyao , Zuo Juncheng
Published: November 2024

A storm surge, classified as an extreme natural disaster, refers to unusual sea level fluctuations induced by severe atmospheric disturbances such as typhoons. Existing reanalysis data, such as ERA5, significantly underestimates the location and maximum wind speed of typhoons. Therefore, this study initially assesses the accuracy of tropical cyclone positions and peak wind speeds in the ERA5 reanalysis dataset. These results are compared against tropical cyclone parameters from the IBTrACS (International Best Track Archive for Climate Stewardship). The position deviation of tropical cyclones in ERA5 is mainly within the range of 10 to 60 km. While the correlation of maximum wind speed is significant, there is still considerable underestimation. A wind field reconstruction model, incorporating tropical cyclone characteristics and a distance correction factor, was employed. This model considers the effects of the surrounding environment during the movement of the tropical cyclone by introducing a decay coefficient. The reconstructed wind field significantly improved the representation of the typhoon eyewall and high-wind-speed regions, showing a closer match with wind speeds observed by the HY-2B scatterometer. Through simulations using the FVCOM (Finite Volume Community Ocean Model) storm surge model, the reconstructed wind field demonstrated higher accuracy in reproducing water level changes at Tanxu, Gaoqiao, and Zhangjiabang stations. During the typhoon’s landfall in Shanghai, the area with the greatest water level increase was primarily located in the coastal waters of Pudong New Area, Shanghai, where the highest total water level reached 5.2 m and the storm surge reached 4 m. The methods and results of this study provide robust technical support and a valuable reference for further storm surge forecasting, marine disaster risk assessment, and coastal disaster prevention and mitigation efforts.
Update on Genitourinary Syndrome of Menopause: A Scoping Review of a Tailored Treatment-Based Approach
Authors: Cuccu Ilaria , Golia D’Augè Tullio , Firulli Ilaria , De Angelis Emanuele , Buzzaccarini Giovanni , D’Oria Ottavia , Besharat Aris Raad , Caserta Donatella , Bogani Giorgio , Muzii Ludovico , Di Donato Violante , Giannini Andrea
Published: November 2024

This scoping review explores the therapeutic strategies available for managing genitourinary syndrome of menopause (GSM), a condition often underdiagnosed and undertreated despite significantly affecting women’s quality of life. GSM results from decreased estrogen levels during menopause, leading to a range of symptoms including vulvovaginal atrophy and urinary tract issues. Material and Methods: we screened the literature for original studies with “menopause”, “hormonal therapy”, “vulvovaginal atrophy”, “urinary incontinence”, “urinary infections”, “genitourinary syndrome”. Results: A total of 451 relevant articles were retrieved. After screening, 19 articles were included in this scoping review. Discussion: First-line treatments typically include lubricants and moisturizers for short-term symptom relief, while unresolved or severe cases may warrant hormonal treatment. Topical hormonal treatments often have fewer side effects than systemic alternatives. Special attention is given to selective estrogen receptor modulators like ospemifene and steroid hormones like dehydroepiandrosterone (DHEA), which have shown beneficial effects on GSM symptoms. Moreover, innovative therapeutic approaches, such as laser treatment, are discussed in the context of their efficacy and accessibility. The safety of GSM hormonal therapy in women with a history or risk of cancer is also addressed, noting the need for more definitive research in this area. While there is a growing demand for tailored therapy, this scoping review emphasizes the importance of effective communication and counseling to allow women to make informed decisions about their treatment. Overall, this review underscores the need for increased awareness and further research into effective treatment options for GSM.
Comparison of the Mitophagy and Apoptosis Related Gene Expressions in Waste Embryo Culture Medium of Female Infertility Types
Authors: Kütük Duygu , Öner Çağrı , Başar Murat , Akcay Berkay , Olcay İbrahim Orçun , Çolak Ertuğrul , Selam Belgin , Cincik Mehmet
Published: November 2024

Mitochondria is an important organelle for the oocyte-to-embryo transition in the early embryonic development period. The oocyte uses mitochondria functionally and its mitochondrial DNA (mtDNA) content as the main energy source in the embryo development at the preimplantation stage. The aim of this study is to compare mitophagic, apoptotic and humanin gene expressions from the culture medium fluid in which embryos are developed and monitored among normoresponder (NOR), polycystic ovary syndrome (PCOS), young and older patients with poor ovarian reserve (POR). The study groups consisted of infertile patients who applied to the Bahçeci Umut IVF Center as NOR (Control), PCOS, POR-Advanced (POR-A) and POR-Young (POR-Y). After the isolation of total RNA from the collected samples, MFN1, MFN2, PINK1, PARKIN, SMN1, SMN2, p53 and Humanin gene expressions were determined by Real Time-PCR. The average age of only the POR-A was determined to be higher than the NOR (p < 0.001). The MFN1, SMN2 (p < 0.05), Humanin and p53 gene expressions (p < 0.001) increased, while PINK1 gene expression decreased (p < 0.05), in the POR-Y compared to the NOR. A decrease in MFN2, PARKIN (p < 0.05) and PINK1 gene expressions was determined in the PCOS compared to the NOR (p < 0.001). Furthermore, a decrease was observed in MFN2, PINK1 (p < 0.001) and Humanin gene expressions compared to the NOR (p < 0.05). The current data are the first in the literature determining the apoptotic and mitophagic status of the oocyte. The current results prove that waste embryo culture fluid may provide a non-invasive profile for important cellular parameters such as mitochondrial dysfunction in female infertility. The evaluation of significant cellular parameters can be performed much earlier without any intervention into the embryo.
Analysis of the Influencing Factors of Aerostatic Bearings on Pneumatic Hammering
Authors: Li Yifei , Huang Weiping , Sang Ran
Published: November 2024

In this study, in order to reveal the influence mechanism of bearing parameters on pneumatic hammering, an aerostatic bearing with a multi-orifice-type restrictor is analyzed. Firstly, the flow field is investigated, and the vortex-induced excitation is discussed in both the frequency and time domains. Then, the frequency-related displacement impedance is analyzed, and the effects of vortex-induced excitation on pneumatic hammering are discussed. Experiments are also conducted for verification. Moreover, the influence of damping on pneumatic hammering is identified. The results show that with larger damping, the risk of pneumatic hammering can be reduced. Finally, the impacts of design parameters on the damping are discussed in detail using an approximate model. Design optimization is considered to achieve the maximum damping, i.e., the minimum risk of pneumatic hammering. The results show that both the air supply pressure and the pocket volume should be minimized. The analysis process provides a reference for the design of bearings to reduce pneumatic hammering.
The Effect of Bi on the Kinetics of Growths, Microstructure and Corrosion Resistance of Hot-Dip Galvanized Coatings
Authors: Kania Henryk , Otmačić Ćurković Helena , Kudláček Jan , Kapitanović Angela , Nackiewicz Joanna , Černý Daniel , Konopkin Grzegorz
Published: November 2024

This paper presents the results of studies on the growth kinetics, microstructure (SEM/EDS) and corrosion behavior of coatings obtained by hot-dip galvanizing process in baths containing Bi additive. The coatings for testing were produced on low-silicon steel in a Zn bath containing 0.04, 0.12 and 0.4 wt.% Bi. The corrosion resistance of the coatings was determined comparatively in standard Neutral Salt Spray Tests (NSST) (ISO 9227) and sulfur dioxide test (SDT) in a humid atmosphere (ISO 22479). Potentiodynamic tests and electrochemical impedance spectroscopy measurements were conducted. It was found that the addition of 0.04 and 0.12 wt.% Bi reduces the total thickness of the coatings and the thickness of intermetallic layers, while the content of 0.4 wt.% Bi in the bath increases the thickness of the layers forming the coating. Direct corrosion tests (NSST and SDT) and electrochemical tests showed that the addition of Bi to the zinc bath reduces the corrosion resistance of the coatings. The corrosion resistance of the coatings decreases with increasing Bi concentration in the zinc bath. In the microstructure of the coatings, it was found that Bi precipitates mainly on the surface of the coating, but also on the cross-section of the outer layer and ζ intermetallic layer. Bi precipitates, due to their cathodic nature, affect the reduction of the corrosion resistance of the coatings with the increase of their content in the bath.