bluuebunny/crossref_metadata_2025 huggingface dataset:
Các nhân tố ảnh hưởng đến sự hài lòng của khách hàng đối với dịch vụ chuyển tiền trực tuyến trên địa bàn Thành phố Hà Nội
Authors: Công Vũ Hà Mi , Nguyễn Hùng Cường
Published: February 2024

Bài viết khám phá các nhân tố ảnh hưởng đến sự hài lòng của khách hàng đối với dịch vụ chuyển tiền trực tuyến trên địa bàn thành phố Hà Nội thông qua vận dụng lý thuyết mô hình SERVPERF. Bài viết sử dụng phương pháp phân tích nhân tố khám phá (EFA), phân tích nhân tố khẳng định (CFA) và phân tích mô hình cấu trúc tuyến tích (SEM) bằng phần mềm SPSS 22 và AMOS 25. Kết quả nghiên cứu cho thấy sự hài lòng của khách hàng đối với dịch vụ chuyển tiền trực tuyến trên địa bàn thành phố Hà Nội chịu ảnh hưởng bởi hai nhân tố: Giá trị chi phí và Độ tin cậy. Từ kết quả nghiên cứu, nhóm tác giả rút ra một số kết luận và hướng nghiên cứu tiếp theo.
Mối quan hệ giữa trách nhiệm xã hội của doanh nghiệp và cam kết với tổ chức qua phân tích trắc nghiệm thư mục
Authors: Mai Thị Thương , Phan Thanh Hải , Phan Tuấn Anh , Hồ Tất Tuân
Published: February 2024

Nghiên cứu này được thiết kế nhằm mục đích khám phá và xác định các hướng nghiên cứu chủ yếu liên quan đến chủ đề về trách nhiệm xã hội của doanh nghiệp (CSR) và cam kết với tổ chức. Dữ liệu được thu thập dựa vào việc truy xuất hệ thống cơ sở dữ liệu Scopus với 212 tài liệu được công bố từ năm 2006 đến 2023. Phương pháp nghiên cứu được thực hiện đó chính là việc phân tích thống kê mô tả, phân tích trắc nghiệm thư mục qua công cụ hỗ trợ là phần mềm VOSviewer 1.6.20. Kết quả nghiên cứu cho thấy các công bố về chủ đề CSR và cam kết với tổ chức được chia thành 5 nhóm tác giả chính với 4 hướng nghiên cứu chủ yếu có liên quan đến việc phát triển các khía cạnh khác nhau. Kết quả nghiên cứu góp phần hệ thống hóa được lý thuyết về chủ đề và từ đó giúp cho các nhà nghiên cứu xác định và xây dựng mô hình phù hợp cho các nghiên cứu trong tương lai.
OTHER COMPREHENSIVE INCOME IN THE INTERNATIONAL FINANCIAL REPORTING STANDARDS: A LITERATURE REVIEW
Authors:   Universitas Airlangga Indonesia, Nugraha Oldiano Tito
Published: February 2024

Research Purposes. This research aims to examine studies regarding investor and contractual profits from Other Comprehensive Income (OCI), as well as its components can provide more evidence regarding whether there are characteristics that differentiate between OCI. Research Methods. This research is research using literature review.The process of reviewing selected articles focuses on understanding the problem, context, complexity and subjectivity of researchers in studying and interpreting OCI’s conceptions and OCI components (and AOCI) and their various relevance that are beneficial to investors in IFRS. Google search, www.semanticcholar.org and onlinelibrary.wiley.com were used to find journal special issues. Research Results and Findings. OCI information and OCI components in the IFRS standard income statement are little use to investors, especially for assessing risk in making investment decisions. Investors should be careful not to overly consider OCI information and OCI components (and AOCI) when making investment decision, including debt contracts.
THE JOURNEY OF MANAGERIAL ACCOUNTING RESEARCH IN SINTA 4-INDEXED JOURNALS
Authors:   Widya Mandala Surabaya Catholic University Indonesia, Tambunan Sihar Tigor Benjamin , Widyaningdyah Agnes Utari ,   Widya Mandala Surabaya Catholic University Indonesia
Published: February 2024

Research Purposes. This study uses a bibliographical approach to describe managerial accounting research (MAR) intention in Indonesia. The research materials are pertinent publications that appeared in SINTA 4-indexed journals between 2011 and 2022. Research Methods. Relevant articles are filtered based on several selection criteria at the Indonesia National Indexer (SINTA), journals, and articles. The metadata of the articles is tidied via Zotero reference management software. The output is CSV and RIS files. MS Excel and Tableau processed CSV files for visualization and qualitative analysis purposes, and RIS files were analyzed by VOSviewer software to create research network maps based on keyword occurrences. Several reputable international journals are used as a comparison. Research Results and Findings. In 2011-2022, 21 of 441 SINTA 4-indexed journals contained 129 articles (6% of 2,300) on managerial accounting topics. These topics form six research clusters with highly varied occurrences, links, intensities, and publication periods. Sustainability-related issues are in the first cluster. In general, SINTA 4-indexed journals in Indonesia, including accounting-specific journals, have not placed managerial accounting as a particular priority. This research produces a bibliographic map of managerial accounting research in the 2011-2012 period, which can be used as a reference for further research, as well as contextual development of managerial accounting practices.
Antiplatelet drugs use situation for acute myocardial infarction patients in Vietnam
Authors:   Hong Ngu District Medical Center, Hong Ngu District, Dong Thap Province, Vietnam., Nguyen Dang-Hien , Nguyen Ngoc-Van Thi ,   College of Pharmacy, Can Tho University of Medicine and Pharmacy, Ninh Kieu District, Can Tho City, Vietnam, Vo Thanh-Toan ,   College of Pharmacy, Can Tho University of Medicine and Pharmacy, Ninh Kieu District, Can Tho City, Vietnam, Pham , Ngoc-Nga ,   College of Pharmacy, Can Tho University of Medicine and Pharmacy, Ninh Kieu District, Can Tho City, Vietnam, Nguyen Trung-Kien ,   College of Medicine, Can Tho University of Medicine and Pharmacy, Ninh Kieu District, Can Tho City, Vietnam.
Published: February 2024

This study aims to evaluate antiplatelet drug use and possible interactions that can occur during acute myocardial infarction treatment. We establish a cross-sectional descriptive study with 380 acute myocardial infarction inpatient cases treated with antiplatelet drugs in Vietnam in 2021. In our study, the mean age is 69.3, and most patients are over sixty-five, accounting for 67.6%. Indicated antiplatelet drugs encompass aspirin (100%), clopidogrel (86.0%), and ticagrelor (42.6%). Dual antiplatelet therapy with aspirin-clopidogrel and aspirin-ticagrelor accounts for 86.0% and 42.6%, respectively. According to drugs.com and medscape.com, the results show three clinically significant combinations:aspirin-enalapril (37.4%), clopidogrel-esomeprazole (31.3%), and aspirin-captopril (20%). Of all discovered drug interactions, 28.1% are estimated as major and 38.8% as moderate (monitor closely). The results show that aspirin is considered the first-line drug, and the aspirin-clopidogrel combination is more common than aspirin-ticagrelor in the treatment of acute myocardial infarction patients. Aspirin-enalapril, clopidogrelesomeprazole, and aspirin-captopril are clinically significant interactions avoided in indicating.
Establishment of a standardized ethanolic extract of Avicennia officinalis leaves in Vietnam
Authors:   College of Pharmacy, Can Tho University of Medicine and Pharmacy, Ninh Kieu District, Can Tho City, Vietnam., Duong Ngan-Tuyet , Nguyen Ngoc-Van Thi ,   College of Pharmacy, Can Tho University of Medicine and Pharmacy, Ninh Kieu District, Can Tho City, Vietnam., Le Thuy- Cam Thi ,   Drug, Cosmetic, Food Quality Control Center of Can Tho City, Ninh Kieu District, Can Tho City, Vietnam, Duong Chau- Ngoc ,   Drug, Cosmetic, Food Quality Control Center of Can Tho City, Ninh Kieu District, Can Tho City, Vietnam
Published: February 2024

The mangrove plant Avicennia officinalis is widespread throughout the subtropical coast and is known for its many pharmacological properties, such as antibacterial, antiulcer, anti-inflammatory, anti-cancer, and antioxidant. This study determined the physicochemical parameters of the standardized extract, qualitative by phytochemical screening and thin layer chromatography (TLC), qualitative total phenolic content, and the content of the marker compound by high-pressure liquid chromatography (HPLC). Compounds such as p-coumaric acid and cinnamic acid have been found to be major phenolic acids in the leaves of this plant. These results provided useful information for the evaluation of the quality of Avicennia officinalis raw materials and its commercial products. The parameters of the ethanol extract of Avicennia officinalis leaves were obtained as follows: the loss of dry weight (10.9±2.09%), total ash content (22.31%), microbiological limits achieved to meet the requirements of medicinal standards, and total heavy metal content not detected. The content of total phenolics determined amounted to 81.83 mg GAE/g extract. The quantification method by HPLC was validated according to AOAC, and the content of two markers in the extract was p-coumaric acid (1.19 mg/g) and cinnamic acid (3.82 mg/g). The outputs of this research will be standardized extracts that’s the source of medicinal plants in Vietnam.
Advances in exercise-induced vascular adaptation: mechanisms, models, and methods
Authors: Sun Hualing , Zhang Yanyan , Shi Lijun
Published: February 2024

Insufficient physical activity poses a significant risk factor for cardiovascular diseases. Exercise plays a crucial role in influencing the vascular system and is essential for maintaining vascular health. Hemodynamic stimuli generated by exercise, such as shear stress and circumferential stress, directly impact vascular structure and function, resulting in adaptive changes. In clinical settings, incorporating appropriate exercise interventions has become a powerful supplementary approach for treating and rehabilitating various cardiovascular conditions. However, existing models for studying exercise-induced vascular adaptation primarily rely on in vivo animal and in vitro cellular models, each with its inherent limitations. In contrast, human research faces challenges in conducting mechanistic analyses due to ethics issues. Therefore, it is imperative to develop highly biomimetic in vitro/ex vivo vascular models that can replicate exercise stimuli in human systems. Utilizing various vascular assessment techniques is also crucial to comprehensively evaluate the effects of exercise on the vasculature and uncover the molecular mechanisms that promote vascular health. This article reviews the hemodynamic mechanisms that underlie exercise-induced vascular adaptation. It explores the advancements in current vascular models and measurement techniques, while addressing their future development and challenges. The overarching goal is to unravel the molecular mechanisms that drive the positive effects of exercise on the cardiovascular system. By providing a scientific rationale and offering novel perspectives, the aim is to contribute to the formulation of precise cardiovascular rehabilitation exercise prescriptions.
Transition metal oxide nanocatalysts for the deoxygenation of palm oil to green diesel
Authors: Guerrero-Corona C. E. , Melo-Banda J. A. , Lam-Maldonado M. , Vega-Ibarra L. A. , Diaz-Zavala N. P. , Meraz-Melo M. A.
Published: February 2024

This study investigated the hydrodeoxygenation of palm oil by different oxide nanocatalysts of transition metals α -Fe2O3, NiO, and NiFe2O4, which were synthesized by hot injection. All nanomaterials were characterized by X-ray diffraction, Fourier transform infrared spectroscopy, and dynamic light dispersion. The catalytic evaluation was performed in a Parr-type reactor at 350°C, 3.5 MPa of H2 pressure, and 3 h of reaction. The liquid product obtained was analyzed by ultraviolet-visible light spectroscopy to identify the n-C16 generated during the reaction. The activity in the deoxygenation of fatty acids to produce n-C16 hydrocarbons has the following order: α-Fe2O3 < NiFe2O4 < NiO.
Fresh perspectives on multimodal argument reconstruction
Authors: Stöckl Hartmut
Published: February 2024

The present contribution exemplifies current models for argument reconstruction on an environmental protection print-ad, identifying deficits in the way the models account for multimodal argumentation. Based on this critical review, three general research perspectives are suggested for making argument reconstruction maximally multimodal: the reach and logic of semiotic modes, multimodal coherence, and genre-specific multimodal discourse structure.
Association between systemic inflammation response index and chronic kidney disease: a population-based study
Authors: Li Xiaowan , Cui Lan , Xu Hongyang
Published: February 2024

Introduction

Our objective was to explore the potential link between systemic inflammation response index (SIRI) and chronic kidney disease (CKD).

Methods

The data used in this study came from the National Health and Nutrition Examination Survey (NHANES), which gathers data between 1999 and 2020. CKD was diagnosed based on the low estimated glomerular filtration rate (eGFR) of less than 60 mL/min/1.73 m2 or albuminuria (urinary albumin-to-creatinine ratio (ACR) of more than 30 mg/g). Using generalized additive models and weighted multivariable logistic regression, the independent relationships between SIRI and other inflammatory biomarkers (systemic immune-inflammation index (SII), monocyte/high-density lipoprotein ratio (MHR), neutrophil/high-density lipoprotein ratio (NHR), platelet/high-density lipoprotein ratio (PHR), and lymphocyte/high-density lipoprotein ratio (LHR)) with CKD, albuminuria, and low-eGFR were examined.

Results

Among the recruited 41,089 participants, males accounted for 49.77% of the total. Low-eGFR, albuminuria, and CKD were prevalent in 8.30%, 12.16%, and 17.68% of people, respectively. SIRI and CKD were shown to be positively correlated in the study (OR = 1.24; 95% CI: 1.19, 1.30). Furthermore, a nonlinear correlation was discovered between SIRI and CKD. SIRI and CKD are both positively correlated on the two sides of the breakpoint (SIRI = 2.04). Moreover, increased SIRI levels were associated with greater prevalences of low-eGFR and albuminuria (albuminuria: OR = 1.27; 95% CI: 1.21, 1.32; low-eGFR: OR = 1.11; 95% CI: 1.05, 1.18). ROC analysis demonstrated that, compared to other inflammatory indices (SII, NHR, LHR, MHR, and PHR), SIRI exhibited superior discriminative ability and accuracy in predicting CKD, albuminuria, and low-eGFR.

Discussion

When predicting CKD, albuminuria, and low-eGFR, SIRI may show up as a superior inflammatory biomarker when compared to other inflammatory biomarkers (SII, NHR, LHR, MHR, and PHR). American adults with elevated levels of SIRI, SII, NHR, MHR, and PHR should be attentive to the potential risks to their kidney health.
Nitric oxide-induced lipophagic defects contribute to testosterone deficiency in rats with spinal cord injury
Authors: Zhuang Yuge , Liu Wenyuan , Chen Feilong , Xie Minyu , Zhang Hanbin , Huang Zicong , Zhang Xiaoyuan , Liu Jinsheng , Ma Ke , Feng Hongrui , Ruan Shipeng , He Jing , Zhang Wansong , Zou Feng , Kang Xiangjin , Fan Yong , Zhang Guofei , Chen Zhenguo
Published: February 2024

Introduction

Males with acute spinal cord injury (SCI) frequently exhibit testosterone deficiency and reproductive dysfunction. While such incidence rates are high in chronic patients, the underlying mechanisms remain elusive.

Methods and results

Herein, we generated a rat SCI model, which recapitulated complications in human males, including low testosterone levels and spermatogenic disorders. Proteomics analyses showed that the differentially expressed proteins were mostly enriched in lipid metabolism and steroid metabolism and biosynthesis. In SCI rats, we observed that testicular nitric oxide (NO) levels were elevated and lipid droplet-autophagosome co-localization in testicular interstitial cells was decreased. We hypothesized that NO impaired lipophagy in Leydig cells (LCs) to disrupt testosterone biosynthesis and spermatogenesis. As postulated, exogenous NO donor (S-nitroso-N-acetylpenicillamine (SNAP)) treatment markedly raised NO levels and disturbed lipophagy via the AMPK/mTOR/ULK1 pathway, and ultimately impaired testosterone production in mouse LCs. However, such alterations were not fully observed when cells were treated with an endogenous NO donor (L-arginine), suggesting that mouse LCs were devoid of an endogenous NO-production system. Alternatively, activated (M1) macrophages were predominant NO sources, as inducible NO synthase inhibition attenuated lipophagic defects and testosterone insufficiency in LCs in a macrophage-LC co-culture system. In scavenging NO (2-4-carboxyphenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (CPTIO)) we effectively restored lipophagy and testosterone levels both in vitro and in vivo, and importantly, spermatogenesis in vivo. Autophagy activation by LYN-1604 also promoted lipid degradation and testosterone synthesis.

Discussion

In summary, we showed that NO-disrupted-lipophagy caused testosterone deficiency following SCI, and NO clearance or autophagy activation could be effective in preventing reproductive dysfunction in males with SCI.
The allocation of crop production resources in the southeast of Iran: the application of the water-energy-food nexus approach
Authors: Badih Barzin H. , Hoseini S. M. , Hashemitabar M. , Mardani Najafabadi M.
Published: February 2024

Introduction: Due to the expansion of population, economic progress, urbanization, increasing food demands, and diversification of food systems, resources are being excessively exploited and degraded. This is compounded by the challenges posed by climate change and limited resources, as well as inadequate management practices. The concept of water-energy-food (WEF) nexus management recognizes the interdependencies among various resources, such as water, food, and energy, in order to promote sustainable resource management. By establishing a harmonious balance among different objectives, this approach aims to safeguard the well-being of both human societies and the environment, ensuring the fulfillment of needs and the preservation of benefits for both parties.

Methods: In this study, the water-energy-food (WEF) nexus approach is applied to the Sistan plain, located in the southeastern region of Iran, to effectively redistribute production resources within the agricultural sector. The methodology employed is multi-objective programming, which incorporates various goals. These objectives encompass maximizing farmer revenue and energy derived from food production (measured in calories), while simultaneously minimizing greenhouse gas (GHG) emissions, irrigation water consumption, and overall energy consumption throughout the 2018–2019 crop year.

Results: The findings of this study demonstrate that implementing the water-energy-food (WEF) nexus approach in the Sistan plain yields positive outcomes. Despite a reduction in the cultivation area, there is a notable shift towards growing more nutritious crops. This shift not only contributes to food security but also increases crop calorie production from 457.16 million to 565.19 million. Consequently, there is a decrease in irrigation water consumption from 261.62 million to 260.48 million cubic meters, energy consumption from 1400.13 million to 1396.81 million MJ per hectare, and greenhouse gas (GHG) emissions from 0.014 million to 0.0139 million tons per hectare.

Discussion: Analyzing the physical and economic productivity reveals that GHG emissions had the highest productivity in terms of both physical and economic measures in Zahak County. As the WEF nexus approach aims to preserve and prevent environmental degradation, it is recommended to implement development and bio-balance policies utilizing this approach to ensure environmental conservation.
Differentiation of regulatory myeloid and T-cells from adult human hematopoietic stem cells after allogeneic stimulation
Authors: Mathew James M. , Sanders Jes M. , Cirocco Robert , Miller Joshua , Leventhal Joseph R.
Published: February 2024

Introduction

Donor hematopoietic stem cell (DHSC) infusions are increasingly being studied in transplant patients for tolerance induction.

Methods

To analyze the fate of infused DHSCs in patients, we developed anin vitroculture system utilizing CD34+DHSCs stimulated with irradiated allogeneic cells in cytokine supplemented medium long-term.

Results

Flow cytometric analyses revealed loss of the CD34 marker and an increase in CD33+myeloid and CD3+T-cell proportion by 10.4% and 72.7%, respectively, after 21 days in culture. T-cells primarily expressed TcR-αβ and were of both CD4+and CD8+subsets. Approximately 80% of CD3+T cells lacked expression of the co-stimulatory receptor CD28. The CD4+compartment was predominated by CD4+CD25+CD127-FOXP3+Tregs (>50% CD4+CD127- compartment) with <1% of all leukocytes exhibiting a CD4+CD127+phenotype. Molecular analyses for T-cell receptor excision circles showed recent and increased numbers of TcR rearrangements in generated T cells over time suggestingde novodifferentiation from DHSCs. CD33+myeloid cells mostly expressed HLA-DR, but lacked expression of co-stimulatory receptors CD80 and CD83. When studied as modulators in primary mixed lymphocyte reactions where the cells used to stimulate the DHSC were used as responders, the DHSC-lines and their purified CD8+, CD4+, CD33+and linage negative subsets inhibited the responses in a dose-dependent and non-specific fashion. The CD8+cell-mediated inhibition was due to direct lysis of responder cells.

Discussion

Extrapolation of these results into the clinical situation would suggest that DHSC infusions into transplant recipients may generate multiple subsets of donor “chimeric” cells and promote recipient Treg development that could regulate the anti-donor immune response in the periphery. These studies have also indicated that T cell maturation can occurin vitroin response to allogeneic stimulation without the pre-requisite of a thymic-like environment or NOTCH signaling stimulatory cell line.
Carbonic anhydrases in the cell wall and plasma membrane of Arabidopsis thaliana are required for optimal plant growth on low CO2
Authors: Weerasooriya Hiruni N. , Longstreth David J. , DiMario Robert J. , Rosati Viviana C. , Cassel Brittany A. , Moroney James V.
Published: February 2024

Introduction: Plants have many genes encoding both alpha and beta type carbonic anhydrases. Arabidopsis has eight alpha type and six beta type carbonic anhydrase genes. Individual carbonic anhydrases are localized to specific compartments within the plant cell. In this study, we investigate the roles of αCA2 and βCA4.1 in the growth of the plant Arabidopsis thaliana under different CO2 regimes.

Methods: Here, we identified the intracellular location of αCA2 and βCA4.1 by linking the coding region of each gene to a fluorescent tag. Tissue expression was determined by investigating GUS expression driven by the αCA2 and βCA4.1 promoters. Finally, the role of these proteins in plant growth and photosynthesis was tested in plants with T-DNA insertions in the αCA2 and βCA4 genes.

Results: Fluorescently tagged proteins showed that αCA2 is localized to the cell wall and βCA4.1 to the plasma membrane in plant leaves. Both proteins were expressed in roots and shoots. Plants missing either αCA2 or βCA4 did not show any growth defects under the conditions tested in this study. However, if both αCA2 and βCA4 were disrupted, plants had a significantly smaller above- ground fresh weight and rosette area than Wild Type (WT) plants when grown at 200 μL L−1 CO2 but not at 400 and 1,000 μL L−1 CO2. Growth of the double mutant plants at 200 μL L−1 CO2 was restoredif either αCA2 or βCA4.1 was transformed back into the double mutant plants.

Discussion: Both the cell wall and plasma membrane CAs, αCA2 and βCA4.1 had to be knocked down to produce an effect on Arabidopsis growth and only when grown in a CO2 concentration that was significantly below ambient. This indicates that αCA2 and βCA4.1 have overlapping functions since the growth of lines where only one of these CAs was knocked down was indistinguishable from WT growth. The growth results and cellular locations of the two CAs suggest that together, αCA2 and βCA4.1 play an important role in the delivery of CO2 and HCO3− to the plant cell.
Exosomal microRNAs in breast cancer: towards theranostic applications
Authors: Gulati Richa , Mitra Tridip , Rajiv Rohan , Rajan Emilda Judith Ezhil , Pierret Chris , Enninga Elizabeth Ann L. , Janardhanan Rajiv
Published: February 2024

Breast cancer is one of the top two reproductive cancers responsible for high rates of morbidity and mortality among women globally. Despite the advancements in the treatment of breast cancer, its early diagnosis remains a challenge. Recent evidence indicates that despite the adroit use of numerous strategies to facilitate rapid and precision-oriented screening of breast cancer at the community level through the use of mammograms, Fine-needle aspiration cytology (FNAC) and biomarker tracking, no strategy has been unequivocally accepted as a gold standard for facilitating rapid screening for disease. This necessitates the need to identify novel strategies for the detection and triage of breast cancer lesions at higher rates of specificity, and sensitivity, whilst taking into account the epidemiologic and social-demographic features of the patients. Recent shreds of evidence indicate that exosomes could be a robust source of biomaterial for the rapid screening of breast cancer due to their high stability and their presence in body fluids. Increasing evidence indicates that the Exosomal microRNAs- play a significant role in modifying the tumour microenvironment of breast cancers, thereby potentially aiding in the proliferation, invasion and metastasis of breast cancer. In this review, we summarize the role of ExomiRs in the tumour microenvironment in breast cancer. These ExomiRs can also be used as candidate biomarkers for facilitating rapid screening and triaging of breast cancer patients for clinical intervention.
Association of immune cell traits with Parkinson’s disease: a Mendelian randomization study
Authors: Song Zhiwei , Li Wangyu , Han Yupeng , Xu Yiya , Ding Haiqi , Wang Yinzhou
Published: February 2024

Background

Immunity and neuroinflammation play crucial roles in the pathogenesis of Parkinson’s disease (PD). Nonetheless, prior investigations into the correlation between immune inflammation and PD have produced varying results. Identifying specific immune cell phenotypes that are truly associated with PD is challenging, and the causal relationship between immune cells and PD remains elusive.

Methods

This study conducted a comprehensive two-sample Mendelian randomization (MR) analysis, employing five distinct analytical approaches, to clarify the causal connection between immune cell characteristics and the risk of PD. Utilizing GWAS data, we investigated the causal relationship between 731 immune cell traits and PD. These immune cell phenotypes encompass absolute cell (AC) counts, median fluorescence intensity (MFI), and relative cell (RC) counts for B cells, cDCs, mature stage T cells, monocytes, myeloid cells, TBNK (T cells, B cells, and natural killer cells), and Tregs, as well as the logistic parameter (MP) for cDCs and TBNK.

Results

The inverse variance weighted (IVW) analysis indicated that Myeloid DCs (p = 0.004), HVEM expression on CD45RA− CD4+ T cells (p = 0.007), CD62L− CD86+ Myeloid DCs (p = 0.015), and HLA DR expression on monocytes (p = 0.019) were associated with a reduced risk of PD. CD14+ CD16+ monocytes (p = 0.005), HLA DR+ NK cells within CD3− lymphocytes (p = 0.023), and CD28 expression on activated & secreting Tregs (p = 0.032) were associated with an increased risk of PD.

Conclusion

This study establishes a causal link between immune cell phenotype and the pathogenesis of PD, identifying several specific immune cell characteristics associated with PD. This could inspire researchers to delve into the pathogenesis of PD at the cellular subtype level, and aid in the identification of potential pharmacological protein targets for PD.
A novel fusion framework of deep bottleneck residual convolutional neural network for breast cancer classification from mammogram images
Authors: Jabeen Kiran , Khan Muhammad Attique , Hameed Mohamed Abdel , Alqahtani Omar , Alouane M. Turki-Hadj , Masood Anum
Published: February 2024

With over 2.1 million new cases of breast cancer diagnosed annually, the incidence and mortality rate of this disease pose severe global health issues for women. Identifying the disease’s influence is the only practical way to lessen it immediately. Numerous research works have developed automated methods using different medical imaging to identify BC. Still, the precision of each strategy differs based on the available resources, the issue’s nature, and the dataset being used. We proposed a novel deep bottleneck convolutional neural network with a quantum optimization algorithm for breast cancer classification and diagnosis from mammogram images. Two novel deep architectures named three-residual blocks bottleneck and four-residual blocks bottle have been proposed with parallel and single paths. Bayesian Optimization (BO) has been employed to initialize hyperparameter values and train the architectures on the selected dataset. Deep features are extracted from the global average pool layer of both models. After that, a kernel-based canonical correlation analysis and entropy technique is proposed for the extracted deep features fusion. The fused feature set is further refined using an optimization technique named quantum generalized normal distribution optimization. The selected features are finally classified using several neural network classifiers, such as bi-layered and wide-neural networks. The experimental process was conducted on a publicly available mammogram imaging dataset named INbreast, and a maximum accuracy of 96.5% was obtained. Moreover, for the proposed method, the sensitivity rate is 96.45, the precision rate is 96.5, the F1 score value is 96.64, the MCC value is 92.97%, and the Kappa value is 92.97%, respectively. The proposed architectures are further utilized for the diagnosis process of infected regions. In addition, a detailed comparison has been conducted with a few recent techniques showing the proposed framework’s higher accuracy and precision rate.
Perioperative immunotherapy for stage II-III non-small cell lung cancer: a meta-analysis base on randomized controlled trials
Authors: Yu Anping , Fu Feng , Li Xiongying , Wu Mengxin , Yu Meijian , Zhang Wenxiong
Published: February 2024

Background

In recent years, we have observed the pivotal role of immunotherapy in improving survival for patients with non-small cell lung cancer (NSCLC). However, the effectiveness of immunotherapy in the perioperative (neoadjuvant + adjuvant) treatment of resectable NSCLC remains uncertain. We conducted a comprehensive analysis of its antitumor efficacy and adverse effects (AEs) by pooling data from the KEYNOTE-671, NADIM II, and AEGEAN clinical trials.

Methods

For eligible studies, we searched seven databases. The randomized controlled trials (RCTs) pertaining to the comparative analysis of combination neoadjuvant platinum-based chemotherapy plus perioperative immunotherapy (PIO) versus perioperative placebo (PP) were included. Primary endpoints were overall survival (OS) and event-free survival (EFS). Secondary endpoints encompassed drug responses, AEs, and surgical outcomes.

Results

Three RCTs (KEYNOTE-671, NADIM II, and AEGEAN) were included in the final analysis. PIO group (neoadjuvant platinum-based chemotherapy plus perioperative immunotherapy) exhibited superior efficacy in OS (hazard ratio [HR]: 0.63 [0.49-0.81]), EFS (HR: 0.61 [0.52, 0.72]), objective response rate (risk ratio [RR]: 2.21 [1.91, 2.54]), pathological complete response (RR: 4.36 [3.04, 6.25]), major pathological response (RR: 2.79 [2.25, 3.46]), R0 resection rate (RR: 1.13 [1.00, 1.26]) and rate of adjuvant treatment (RR: 1.08 [1.01, 1.15]) compared with PP group (neoadjuvant platinum-based chemotherapy plus perioperative placebo). In the subgroup analysis, EFS tended to favor the PIO group in almost all subgroups. BMI (>25), T stage (IV), N stage (N1-N2) and pathological response (with pathological complete response) were favorable factors in the PIO group. In the safety assessment, the PIO group exhibited higher rates of serious AEs (28.96% vs. 23.51%) and AEs leading to treatment discontinuation (12.84% vs. 5.81%). Meanwhile, although total adverse events, grade 3-5 adverse events, and fatal adverse events tended to favor the PP group, the differences were not statistically significant.

Conclusion

PIO appears to be superior to PP for resectable stage II-III NSCLC, demonstrating enhanced survival and pathological responses. However, its elevated adverse event (AE) rate warrants careful consideration.

Systematic review registration

https://www.crd.york.ac.uk/PROSPERO/#recordDetails, identifier CRD42023487475.
DIAPH3 predicts survival of patients with MGMT-methylated glioblastoma
Authors: Chehade Georges , El Hajj Nady , Aittaleb Mohamed , Alkailani Maisa I. , Bejaoui Yosra , Mahdi Asma , Aldaalis Arwa A. H. , Verbiest Michael , Lelotte Julie , Ruiz-Reig Nuria , Durá Irene , Raftopoulos Christian , Tajeddine Nicolas , Tissir Fadel
Published: February 2024

Background

Glioblastoma is one of the most aggressive primary brain tumors, with a poor outcome despite multimodal treatment. Methylation of the MGMT promoter, which predicts the response to temozolomide, is a well-established prognostic marker for glioblastoma. However, a difference in survival can still be detected within the MGMT methylated group, with some patients exhibiting a shorter survival than others, emphasizing the need for additional predictive factors.

Methods

We analyzed DIAPH3 expression in glioblastoma samples from the cancer genome atlas (TCGA). We also retrospectively analyzed one hundred seventeen histological glioblastomas from patients operated on at Saint-Luc University Hospital between May 2013 and August 2019. We analyzed the DIAPH3 expression, explored the relationship between mRNA levels and Patient’s survival after the surgical resection. Finally, we assessed the methylation pattern of the DIAPH3 promoter using a targeted deep bisulfite sequencing approach.

Results

We found that 36% and 1% of the TCGA glioblastoma samples exhibit copy number alterations and mutations in DIAPH3, respectively. We scrutinized the expression of DIAPH3 at single cell level and detected an overlap with MKI67 expression in glioblastoma proliferating cells, including neural progenitor-like, oligodendrocyte progenitor-like and astrocyte-like states. We quantitatively analyzed DIAPH3 expression in our cohort and uncovered a positive correlation between DIAPH3 mRNA level and patient’s survival. The effect of DIAPH3 was prominent in MGMT-methylated glioblastoma. Finally, we report that the expression of DIAPH3 is at least partially regulated by the methylation of three CpG sites in the promoter region.

Conclusion

We propose that combining the DIAPH3 expression with MGMT methylation could offer a better prediction of survival and more adapted postsurgical treatment for patients with MGMT-methylated glioblastoma.
Bone health in children with primary hyperoxaluria type 1 following liver and kidney transplantation
Authors: Büscher Rainer , Pape Lars , Büscher Anja K.
Published: February 2024

Background

Primary hyperoxaluria type 1 is characterized by hepatic oxalate overproduction, leading to nephrocalcinosis, kidney stones, kidney failure and systemic oxalosis, including oxalate osteopathy. Combined liver-kidney transplantation (CLKT) and kidney after liver transplantation (KALT) were established therapeutic options to stop the devastating consequences of oxalate bone disease.

Methods

We describe a retrospective cohort of 10 children with PH1who were referred to our hospital from different countries for combined transplantation. Demographic and clinical data were collected and symptoms of bone disease, conventional radiological examinations, plasma oxalate levels and other determinants of calcium-phosphate metabolism were compared pre and post transplantation.

Results

Ten patients (7 male, median age 5.8 years, median follow-up time 8.1 years) were included in this study. Seven patients were diagnosed with infantile oxalosis and 9 patients received an intensified dialysis regime prior to transplantation. In one patient the transplanted kidney never achieved primary function and the boy remained on HD. All other patients remained without graft failure and retained stable kidney and liver function. Prior to transplantation, seven patients suffered from severe skeletal pain and three children presented with 1–3 series of pathological fractures. Pathological fractures did no longer occur in children who underwent successful CLKT or KALT. Plasma oxalate levels dropped within 6 months following Tx. Determinants of calcium-phosphorus metabolism did not differ significantly in comparison to other HD children. Seven of ten children showed a restricted growth at the time of transplantation and presented a moderate catch-up-growth at the time of last follow-up.

Conclusions

Patients with PH1 suffer from severe consequences of a disturbed bone metabolism. However, bone health and growth can partially improve following CLKT/KALT.
β3-Adrenoceptor as a new player in the sympathetic regulation of the renal acid–base homeostasis
Authors: Milano Serena , Saponara Ilenia , Gerbino Andrea , Lapi Dominga , Lela Ludovica , Carmosino Monica , Dal Monte Massimo , Bagnoli Paola , Svelto Maria , Procino Giuseppe
Published: February 2024

Efferent sympathetic nerve fibers regulate several renal functions activating norepinephrine receptors on tubular epithelial cells. Of the beta-adrenoceptors (β-ARs), we previously demonstrated the renal expression of β3-AR in the thick ascending limb (TAL), the distal convoluted tubule (DCT), and the collecting duct (CD), where it participates in salt and water reabsorption. Here, for the first time, we reported β3-AR expression in the CD intercalated cells (ICCs), where it regulates acid–base homeostasis. Co-localization of β3-AR with either proton pump H+-ATPase or Cl−/HCO3− exchanger pendrin revealed β3-AR expression in type A, type B, non-A, and non-B ICCs in the mouse kidney. We aimed to unveil the possible regulatory role of β3-AR in renal acid–base homeostasis, in particular in modulating the expression, subcellular localization, and activity of the renal H+-ATPase, a key player in this process. The abundance of H+-ATPase was significantly decreased in the kidneys of β3-AR−/− compared with those of β3-AR+/+ mice. In particular, H+-ATPase reduction was observed not only in the CD but also in the TAL and DCT, which contribute to acid–base transport in the kidney. Interestingly, we found that in in vivo, the absence of β3-AR reduced the kidneys’ ability to excrete excess proton in the urine during an acid challenge. Using ex vivo stimulation of mouse kidney slices, we proved that the β3-AR activation promoted H+-ATPase apical expression in the epithelial cells of β3-AR-expressing nephron segments, and this was prevented by β3-AR antagonism or PKA inhibition. Moreover, we assessed the effect of β3-AR stimulation on H+-ATPase activity by measuring the intracellular pH recovery after an acid load in β3-AR-expressing mouse renal cells. Importantly, β3-AR agonism induced a 2.5-fold increase in H+-ATPase activity, and this effect was effectively prevented by β3-AR antagonism or by inhibiting either H+-ATPase or PKA. Of note, in urine samples from patients treated with a β3-AR agonist, we found that β3-AR stimulation increased the urinary excretion of H+-ATPase, likely indicating its apical accumulation in tubular cells. These findings demonstrate that β3-AR activity positively regulates the expression, plasma membrane localization, and activity of H+-ATPase, elucidating a novel physiological role of β3-AR in the sympathetic control of renal acid–base homeostasis.
Towards a practical threat assessment methodology for crop landraces
Authors: Almeida Maria João , Barata Ana Maria , De Haan Stef , Joshi Bal Krishna , Brehm Joana Magos , Yazbek Mariana , Maxted Nigel
Published: February 2024

Crop landraces (LR), the traditional varieties of crops that have been maintained for millennia by repeated cycles of planting, harvesting, and selection, are genetically diverse compared to more modern varieties and provide one of the key components for crop improvement due to the ease of trait transfer within the crop species. However, LR diversity is increasingly threatened with genetic erosion and extinction by replacement with improved cultivars, lack of incentives for farmers to maintain traditional agricultural systems, and rising threats from climate change. Their active conservation is necessary to maintain this critical resource. However, as there are hundreds of thousands of LR and millions of LR populations for crops globally, active conservation is complex and resource-intensive. To assist in implementation, it is useful to be able to prioritise LR for conservation action and an obvious means of prioritisation is based on relative threat assessment. There have been several attempts to propose LR threat assessment methods, but none thus far has been widely accepted or applied. The aim of this paper is to present a novel, practical, standardised, and objective methodology for LR threat assessment derived from the widely applied IUCN Red Listing for wild species, involving the collation of time series information for LR population range, LR population trend, market, and farmer characteristics and LR context information. The collated information is compared to a set of threat criteria and an appropriate threat category is assigned to the LR when a threshold level is reached. The proposed methodology can be applied at national, regional, or global levels and any crop group.
Variations in species diversity patterns and community assembly rules among vegetation types in the karst landscape
Authors: Meng Longchenxi , Li Yong , Chen Luyao , Sui Mingzhen , Zhang Guangqi , Liu Qingfu , Chen Danmei , Wu Yuhang , Yang Zeyu , Chen Shiren , Yang Rui , Zang Lipeng
Published: February 2024

The various vegetation types in the karst landscape have been considered the results of heterogeneous habitats. However, the lack of a comprehensive understanding of regional biodiversity patterns and the underlying ecological processes limits further research on ecological management. This study established forest dynamic plots (FDPs) of the dominant vegetation types (shrubland, SL; mixed tree and shrub forest, MTSF; coniferous forest, CF; coniferous broadleaf mixed forest, CBMF; and broadleaf forest, BF) in the karst landscape and quantified the species diversity patterns and potential ecological processes. The results showed that in terms of diversity patterns, the evenness and species richness of the CF community were significantly lower than other vegetation types, while the BF community had the highest species richness. The other three vegetation types showed no significant variation in species richness and evenness. However, when controlling the number of individuals of FDPs, the rarefied species richness showed significant differences and ranked as BF > SL > MTSF > CBMF > CF, highlighting the importance of considering the impacts of abundance. Additionally, the community assembly of climax communities (CF or BF) was dominated by stochastic processes such as species dispersal or species formation, whereas deterministic processes (habitat filtering) dominated the secondary forests (SL, MTSF, and CBMF). These findings proved that community assembly differs mainly between the climax community and other communities. Hence, it is crucial to consider the biodiversity and of the potential underlying ecological processes together when studying regional ecology and management, particularly in heterogeneous ecosystems.
The Pro-197-Thr mutation in the ALS gene confers novel resistance patterns to ALS-inhibiting herbicides in Bromus japonicus in China
Authors: Liu Leicheng , Wu Lamei , Li Zongfang , Fang Yuhang , Ju Boming , Zhang Sisi , Bai Lianyang , Pan Lang
Published: February 2024

Introduction

Bromus japonicus is one of the most notorious agricultural weeds in China. The long-term use of ALS-inhibiting herbicides has led to rapid evolution of herbicide resistance in B. japonicus. B. japonicus population (BJ-R) surviving mesosulfuron-methyl treatment was collected from wheatland. Here, we aimed to confirm the resistance mechanisms in this putative resistant population.

Methods

The dose-reponse tests were used to test the resistance level of the B. japonicus to ALS-inhibiting herbicides. Pretreatment with P450 and GST inhibitors and GST activity assays were used to determine whether P450 or GST was involved in the resistance of the BJ-R population. Sanger sequencing was used to analyse the ALS mutation of the BJ-R population. RT-qPCR was used to confirm the the expression levels of the ALS gene in mesosulfuron-methyl -resistant (BJ-R) and-susceptible (BJ-S) B. japonicus. An in vitro ALS activity assay was used to determine the ALS activity of the BJ-R and BJ-S populations. Homology modelling and docking were used to determine the binding energy of the BJ-R and BJ-S populations with ALS-inhibiting herbicides.

Results

B. japonicus population (BJ-R) was confirmed to be 454- and 2.7-fold resistant to the SU herbicides mesosulfuron-methyl and nicosulfuron, and 7.3-, 2.3-, 1.1- and 10.8-fold resistant to the IMI herbicide imazamox, the TP herbicide penoxsulam, the PTB herbicide pyribenzoxim and the SCT herbicide flucarbazone-sodium, respectively, compared with its susceptible counterpart (BJ-S). Neither a P450 inhibitor nor a GST inhibitor could reverse the level of resistance to mesosulfuron-methyl in BJ-R. In addition, no significant differences in GST activity were found between the BJ-R and BJ-S. ALS gene sequencing revealed a Pro-197-Thr mutation in BJ-R, and the gene expression had no significant differences between the BJ-R and BJ-S. The ALS activity of BJ-R was 106-fold more tolerant to mesosulfuron-methyl than that of BJ-S. Molecular docking showed that the binding energy of the ALS active site and mesosulfuron-methyl was changed from -6.67 to -4.57 kcal mol-1 due to the mutation at position 197.

Discussion

These results suggested that the Pro-197-Thr mutation was the main reason for the high resistance level of BJ-R to mesosulfuron-methyl. Unlike previous reports of the cross-resistance pattern conferred by this mutation, we firstly documented that the Pro-197-Thr mutation confers broad cross-resistance spectrums to ALS-inhibiting herbicides in B. japonicus.