bluuebunny/crossref_metadata_2025 huggingface dataset:
Mapping the Burden of Fungal Diseases in the United Arab Emirates
Authors: Al Dhaheri Fatima , Thomsen Jens , Everett Dean , Denning David W.
Published: May 2024

The United Arab Emirates has very little data on the incidence or prevalence of fungal diseases. Using total and underlying disease risk populations and likely affected proportions, we have modelled the burden of fungal disease for the first time. The most prevalent serious fungal conditions are recurrent vulvovaginitis (~190,000 affected) and fungal asthma (~34,000 affected). Given the UAE’s low prevalence of HIV, we estimate an at-risk population of 204 with respect to serious fungal infections with cryptococcal meningitis estimated at 2 cases annually, 15 cases of Pneumocystis pneumonia (PCP) annually, and 20 cases of esophageal candidiasis in the HIV population. PCP incidence in non-HIV patients is estimated at 150 cases annually. Likewise, with the same low prevalence of tuberculosis in the country, we estimate a total chronic pulmonary aspergillosis prevalence of 1002 cases. The estimated annual incidence of invasive aspergillosis is 505 patients, based on local data on rates of malignancy, solid organ transplantation, and chronic obstructive pulmonary disease (5.9 per 100,000). Based on the 2022 annual report of the UAE’s national surveillance database, candidaemia annual incidence is 1090 (11.8/100,000), of which 49.2% occurs in intensive care. Fungal diseases affect ~228,695 (2.46%) of the population in the UAE.
Comprehensive Review of Tinea Capitis in Adults: Epidemiology, Risk Factors, Clinical Presentations, and Management
Authors: Hill Rachel C. , Gold Jeremy A. W. , Lipner Shari R.
Published: May 2024

Tinea capitis is a fungal infection of the scalp and hair caused by dermatophyte molds, that most often affects children and may also affect adults. Previous estimates suggest that between 3% and 11% of all tinea capitis cases worldwide occur in adults, although updated epidemiological studies are needed to reassess the prevalence of tinea capitis in adult populations specifically. Postmenopausal adult women are most often affected by tinea capitis, with African American or Black women particularly at risk. Adults who experience crowded living conditions, who live in close proximity to animals, who are immunosuppressed, and/or who live in households with affected children are at greatest risk of infection. Tinea capitis can be non-inflammatory or inflammatory in nature, and the subtype affects the extent and severity of clinical symptoms. Fungal culture and potassium hydroxide preparations are the most commonly used diagnostic tools. Trichoscopy, defined as dermoscopic imaging of the scalp and hair, is a useful adjunct to the physical examination. The mainstay of therapy is oral antifungal therapy, and topical therapy alone is not recommended. Since tinea capitis infection is uncommon in adults, there are no widely accepted treatment guidelines. Rather, the same medications used for tinea capitis infection among children are recommended for adults at varying doses, including griseofulvin, and terbinafine, and, less commonly, itraconazole and fluconazole. The prognosis for tinea capitis in adults is typically excellent when prompt and adequate treatment is administered; however, delayed diagnosis or inadequate treatment can result in scarring alopecia. Over the past decade, dermatophyte infections resistant to treatment with topical and oral antifungal agents have emerged. While tinea capitis infections resistant to antifungal therapy have been rarely reported to date, antifungal resistance is rising among superficial fungal infections in general, and antifungal stewardship is necessary to ensure that resistance to treatment does not develop among dermatophytes that cause tinea capitis.
Ethnic and Gender Variations in Ischemic Stroke Patterns among Arab Populations in Northern Israel: A Preliminary Exploration towards Culturally Aware Personalized Stroke Care
Authors: Ryder Chen Hanna , Gal Carmit , Barkay Gili , Raveh Amsalem Shani , Sarusi Ziv , Shahien Radi , Badarny Samih
Published: May 2024

The Galilee region of Israel boasts a rich ethnic diversity within its Arab population, encompassing distinct Muslim, Christian, Druze, and Bedouin communities. This preliminary exploratory study uniquely examined potential ethnic and gender differences in ischemic stroke characteristics across these Arab subgroups, which are seldom investigated separately in Israel and are typically studied as a homogeneous “Arab” sector, despite significant variations in their ethnicity, culture, customs, and genetics. The current study aimed to comparatively evaluate stroke characteristics, including recurrence rates, severity, and subtypes, within and across these distinct ethnic groups and between genders. When examining the differences in stroke characteristics between ethnic groups, notable findings emerged. The Bedouin population exhibited significantly higher rates of recurrent strokes than Muslims (M = 0.55, SD = 0.85 vs. M = 0.25, SD = 0.56; p < 0.05). Large vessel strokes were significantly more prevalent among Christians (30%) than Druze (9.9%; p < 0.05). Regarding gender differences within each ethnic group, several disparities were observed. Druze women were six times more likely to experience moderate to severe strokes than their male counterparts (p < 0.05). Interestingly, Druze women also exhibited a higher representation of cardio-embolic stroke (19.8%) compared with Druze men (4.6%; p < 0.001). These findings on the heterogeneity in stroke characteristics across Arab ethnic subgroups and by gender underscore the need to reconsider the approach that views all ethnic groups comprising the Arab sector in Israel as a homogeneous population; instead, they should be investigated as distinct communities with unique stroke profiles, requiring tailored culturally aware community-based prevention programs and personalized therapeutic models. The identified patterns may guide future research to develop refined, individualized, and preventive treatment approaches targeting the distinct risk factors, healthcare contexts, and prevention needs of these diverse Arab populations.
Minimally Invasive Isolated and Hybrid Surgical Revascularization for Multivessel Coronary Disease: A Single-Center Long-Term Follow-Up
Authors: Torre Tiziano , Pozzoli Alberto , Valgimigli Marco , Leo Laura Anna , Toto Francesca , Muretti Mirko , Birova Sara , Ferrari Enrico , Pedrazzini Giovanni , Demertzis Stefanos
Published: May 2024

Introduction: Some evidence suggests that surgical minimally invasive (MIDCAB) and hybrid coronary revascularization (HCR) are safe and potentially effective at short-term follow-up. Data on long-term outcomes are more limited and inconclusive. Methods: Between February 2013 and December 2023, a total of 1997 patients underwent surgical coronary artery revascularization at our institution, of whom, 92 (4.7%) received left anterior mini-thoracotomy access (MIDCAB), either isolated (N = 78) or in combination with percutaneous coronary intervention (N = 14, HCR group). Results: After a median follow-up of 75 months (range 3.1: 149 months), cardiac mortality was 0% while overall mortality was 3%, with one in-hospital mortality and two additional late deaths. Conversion to sternotomy happened in two patients (2.1%), and surgical re-explorations occurred in five patients (4.6%), of whom three for bleeding and two for graft failure. All patients received left internal mammary (LIMA) to left anterior descending artery (LAD) grafting (100%). In the HCR group, 10 patients (72%) showed percutaneous revascularization (PCI) after MIDCAB, showing PCI on a mean of 1.6 ± 0.6 vessels and implanting 2.1 ± 0.9 drug-eluting stents. Conclusions: MIDCAB, in isolation or in association with hybrid coronary revascularization, is associated with encouraging short- and long-term results in selected patients discussed within a dedicated heart-team.
Awareness of Proton Pump Inhibitor Adverse Events and Treatment Pattern Change According to Physician Practice: A National Questionnaire Study in Korea
Authors: Choi Yong Hoon , Seo Seung In , Jung Da Hyun , Kim Joon Sung , Kim Seung Young , Lim Hyun Chul , Youn Young Hoon
Published: May 2024

Although adverse events of proton pump inhibitors (PPIs) have been reported, there are few studies on physicians’ perceptions. We aimed to investigate physicians’ awareness of PPI-related adverse events and changes in treatment patterns according to their practice. We conducted an online survey of physicians using a 15-item questionnaire. The survey queried respondents’ demographic information, PPI prescription patterns, perceptions, and concerns on the reported PPI-related adverse events. Concerns regarding the adverse events of PPI were assessed by dividing them into possibilities and medical causality. Of the 450 respondents, 430 were specialists, and 232 were gastroenterologists. A total of 87.8% of the respondents were generally or well aware of the adverse effects of PPI, 29.1% considered side effects when prescribing PPI, and 14.6% explained them to patients. Specialists were more aware of the side effects of PPI than general practitioners (p = 0.005), and gastroenterologists were more aware of the side effects of PPI than non-gastroenterologists (p < 0.001). However, gastroenterologists explained less to patients (p = 0.001) and preferred to reduce the dose of PPI rather than discontinue it. The adverse events that were recognized as having the highest probability of occurrence and strongest association with PPI use were bone diseases, Clostridium difficile infection, gastrointestinal infection, pneumonia, and interactions with anti-thrombotic drugs. Physicians’ awareness of PPI-related adverse events and treatment patterns differed significantly according to their positions and practice. Although a number of adverse events of PPIs were reported, physicians seem to accept their significance differently according to their specialty and practice patterns.
Prognostic Value of Fibrosis 4 (FIB-4) Index in Sepsis Patients
Authors: Albayrak Tuna , Yuksel Beyza
Published: May 2024

Background: Sepsis remains a major health challenge worldwide, characterized by a dysregulated host response to infection, leading to high mortality and morbidity in intensive care units (ICUs). The Fibrosis 4 (FIB-4) index, originally developed to assess liver fibrosis in hepatitis C patients, has recently been explored for its potential prognostic value in sepsis patients. Method: this study retrospectively analyzed 309 sepsis patients admitted to the Internal Medicine and An-aesthesia ICUs between 12 December 2021 and 15 December 2023 to investigate the relationship between FIB-4 levels, the Acute Physiology and Chronic Health Evaluation (APACHE), the Sequential Organ Failure Assessment (SOFA), and clinical outcomes. Results: This study found that higher FIB-4 measurements were statistically significantly associated with increased 28-day mortality, with a cut-off value of 4.9, providing a sensitivity of 54.92% and specificity of 74.25%. Logistic regression analysis indicated that elevated FIB-4 levels were a significant predictor of early mortality, suggesting that the FIB-4 index could serve as a valuable prognostic tool in assessing the severity and prognosis of sepsis patients. Conclusions: by elucidating the potential role of the FIB-4 index in sepsis prognosis, this study contributes to the ongoing efforts to improve risk stratification and enhance patient care in sepsis management.
The Impact of Stock Price Crash Risk on Bank Dividend Payouts
Authors: Jin Justin Yiqiang , Liu Yi
Published: May 2024

In this study, we examine whether and how banks employ dividend payout policies in response to the risk of stock price crashes. Using a sample of U.S. banks, we find that banks increase their dividend payouts when faced with a higher risk of stock price crashes. In addition, we find that well-capitalized banks tend to pay more dividends when the risk of a stock price crash is elevated. This aligns with the regulatory pressure theory that banks distribute dividends when they have sufficient capital that meets or exceeds the regulatory standards. This is also in line with the signaling theory that dividend payments reflect a bank’s confidence in its financial health. Furthermore, we find that financially opaque banks tend to make more dividend payments when they are at a higher risk of stock price crashes. This supports the agency cost theory, suggesting that dividends counterbalance the need to monitor bank managers in less transparent reporting environments.
An Integrating Framework for Biomass and Carbon Stock Spatialization and Dynamics Assessment Using Unmanned Aerial Vehicle LiDAR (LiDAR UAV) Data, Landsat Imagery, and Forest Survey Data in the Mediterranean Cork Oak Forest of Maamora
Authors: Fadil Sanaa , Sebari Imane , Ajerame Moulay Mohamed , Ajeddour Rayhana , El Maghraoui Ibtihal , Ait El kadi Kenza , Zefri Yahya , Jabrane Mouad
Published: May 2024

Spatialization of biomass and carbon stocks is essential for a good understanding of the forest stand and its characteristics, especially in degraded Mediterranean cork oak forests. Furthermore, the analysis of biomass and carbon stock changes and dynamics is essential for understanding the carbon cycle, in particular carbon emissions and stocks, in order to make projections, especially in the context of climate change. In this research, we use a multidimensional framework integrating forest survey data, LiDAR UAV data, and extracted vegetation indices from Landsat imagery (NDVI, ARVI, CIG, etc.) to model and spatialize cork oak biomass and carbon stocks on a large scale. For this purpose, we explore the use of univariate and multivariate regression modeling and examine several types of regression, namely, multiple linear regression, stepwise linear regression, random forest regression, simple linear regression, logarithmic regression, and quadratic and cubic regression. The results show that for multivariate regression, stepwise regression gives good results, with R2 equal to 80% and 65% and RMSE equal to 2.59 and 1.52 Mg/ha for biomass and carbon stock, respectively. Random forest regression, chosen as the ML algorithm, gives acceptable results, explaining 80% and 60% of the variation in biomass and carbon stock, respectively, and an RMSE of 2.74 and 1.72 Mg/ha for biomass and carbon stock, respectively. For the univariate regression, the simple linear regression is chosen because it gives satisfactory results, close to those of the quadratic and cubic regressions, but with a simpler equation. The vegetation index chosen is ARVI, which shows good performance indices, close to those of the NDVI and CIG. The assessment of biomass and carbon stock changes in the study area over 35 years (1985–2020) showed a slight increase of less than 10 Mg/ha and a decrease in biomass and carbon stock over a large area.
Impacts of Wildlife Artificial Water Provisioning in an African Savannah Ecosystem: A Spatiotemporal Analysis
Authors: Mpalo Morati , Basupi Lenyeletse Vincent , Tsidu Gizaw
Published: May 2024

The use of artificial water points for wildlife in African savannah ecosystems has been widely criticised for affecting the distribution of wildlife and initiating changes in the heterogeneity of natural landscapes. We examined the spatiotemporal variations in the landscape before and after the installation of an artificial water point by integrating the analysis of vegetation and soil spectral response patterns with a supervised learning random forest model between 2002 and 2022 in Chobe Enclave, Northern Botswana. Our results revealed that the study area is characterised by animal species such as Equus quagga, Aepyceros melampus, and Loxodonta africana. The findings also showed that the main vegetation species in the study area landscape include Combretum elaeagnoides, Vachellia luederitzii, and Combretum hereroense. The artificial water point induced disturbances on a drought-vulnerable landscape which affected vegetation heterogeneity by degrading the historically dominant vegetation cover types such as Colophospermum mopane, Dichrostachys cinerea, and Cynodon dactylon. The immediate years following the artificial water point installation demonstrated the highest spectral response patterns by vegetation and soil features attributed to intense landscape disturbances due to abrupt high-density aggregation of wildlife around the water point. Landscapes were strongly homogenised in later years (2022), as shown by overly overlapping spectral patterns owing to an increase in dead plant-based material and senescent foliage due to vegetation toppling and trampling. The landscape disturbances disproportionately affected mopane-dominated woodlands compared to other vegetation species as indicated by statistically significant land cover change obtained from a random forest classification. The woodlands declined significantly (p < 0.05) within 0–0.5 km, 0.5–1 km, 1–5 km, and 5–10 km distances after the installation of the water point. The results of this study indicate that continuous nonstrategic and uninformed use of artificial water points for wildlife will trigger ecological alterations in savannah ecosystems.
A Geospatial Model of Periurbanization—The Case of Three Intermediate-Sized and Subregional Cities in Chile
Authors: Ubilla-Bravo Gerardo Francisco
Published: May 2024

Throughout the 20th century and in the first decades of the 21st century, the geospatial dynamic exhibiting the highest rate of change globally corresponds to urban expansion surrounding metropolitan areas and large cities. Around intermediate-sized cities, there have also been rapid changes in their geographical space, but study in these areas has had less academic attention and development. Considering this context, this article intends to analyze the dynamics in the periurbanization of communes with intermediate-sized cities. In this study, three geographical criteria were defined for the definition of the study area and seven geospatial indicators of sociodemographic, socioeconomic and land occupation categories, with the purpose of determining the composition of the periurbanization process. Finally, the discussion presents a perspective on the dynamics of periurbanization, the interpretation of future projections identifying three geospatial phenomena and a proposal for a geospatial chorematic model with the composition of periurbanization, based on three subregional intermediate-sized cities in the Metropolitan Region of Santiago de Chile. This research contributes new reflections to the debate around spatial planning and periurban research in Latin America and the Global South.
Role of Natural Binding Proteins in Therapy and Diagnostics
Authors: Eigenfeld Marco , Lupp Kilian F. M. , Schwaminger Sebastian P.
Published: May 2024

This review systematically investigates the critical role of natural binding proteins (NBPs), encompassing DNA-, RNA-, carbohydrate-, fatty acid-, and chitin-binding proteins, in the realms of oncology and diagnostics. In an era where cancer continues to pose significant challenges to healthcare systems worldwide, the innovative exploration of NBPs offers a promising frontier for advancing both the diagnostic accuracy and therapeutic efficacy of cancer management strategies. This manuscript provides an in-depth examination of the unique mechanisms by which NBPs interact with specific molecular targets, highlighting their potential to revolutionize cancer diagnostics and therapy. Furthermore, it discusses the burgeoning research on aptamers, demonstrating their utility as ‘nucleic acid antibodies’ for targeted therapy and precision diagnostics. Despite the promising applications of NBPs and aptamers in enhancing early cancer detection and developing personalized treatment protocols, this review identifies a critical knowledge gap: the need for comprehensive studies to understand the diverse functionalities and therapeutic potentials of NBPs across different cancer types and diagnostic scenarios. By bridging this gap, this manuscript underscores the importance of NBPs and aptamers in paving the way for next-generation diagnostics and targeted cancer treatments.
What Is New about the Semimembranosus Distal Tendon? Ultrasound, Anatomical, and Histological Study with Clinical and Therapeutic Application
Authors: Iglesias-Chamorro Pere , Pérez-Bellmunt Albert , Ortiz-Miguel Sara , Möller Ingrid , Blasi Juan , Ortiz-Sagristà Juan , Martinoli Carlo , Sanjuan Xavier , Miguel-Pérez Maribel
Published: May 2024

The semimembranosus muscle inserts into several tendons that are associated with some pathologies. Although ultrasound is useful for studying, diagnosing, and managing these pathologies, the correct interpretation of any images requires a clear knowledge of the related anatomical structures and the inter-related functions. We studied 38 cryopreserved non-paired knees from adult anatomical specimens and 4 non-paired knees from 29 to 38-week-old fetuses. The semimembranosus muscle and its tendons were located, observed, and injected under ultrasound guidance. The macroscopic anatomy was studied using dissection and anatomical cuts and the tendons were analyzed histologically. Measurements of muscle were taken 10 cm from the medial epicondyle and just before the tendon divided. The ultrasound facilitated the identification of the different divisions of the tendon of semimembranosus muscle and the rotation of the muscle and tendon from medial to posterior. An anatomical study confirmed this rotation and revealed an average width, thickness, and diameter of 38.29 mm, 14.36 mm, and 112.64 mm, respectively. Important relationships were observed between the divisions of the main tendons and the medial collateral ligament, the posterior side of the knee and popliteus muscle. This information can help to explain knee pathologies and facilitate rehabilitation after surgery.
Myelodysplastic Syndrome: Clinical Characteristics and Significance of Preclinically Detecting Biallelic Mutations in the TET2 Gene
Authors: Danishevich Anastasiia , Chegodar Anzhelika , Bodunova Natalia , Konovalov Fedor , Nefedova Maria , Kremneva Natalya , Kurbanov Nizhat , Bilyalov Airat , Nikolaev Sergey , Khatkov Igor , Dudina Galina
Published: May 2024

Myelodysplastic syndrome (MDS) is a clonal disease derived from hematopoietic stem cells, characterized by ineffective hematopoiesis (resulting in peripheral blood cytopenia) and an increased risk of transformation into acute myeloid leukemia. MDS is caused by a complex combination of genetic mutations resulting in a heterogeneous genotype. Genetic studies have identified a set of aberrations that play a central role in the pathogenesis of MDS. In this article, we present a clinical case of MDS transformation into acute myeloid leukemia in the context of two cell lines exhibiting morphological, immunophenotypic, and dysmyelopoiesis markers and the presence of two heterozygous mutations in the TET2 gene.
Autoimmune Hepatitis Management: Recent Advances and Future Prospects
Authors: Sierra Rebeca , Marenco-Flores Ana , Alsaqa Marwan , Barba Romelia , Cuellar-Lobo Marcela , Barberan Carla , Sierra Leandro
Published: May 2024

Autoimmune hepatitis (AIH) is a varied inflammatory chronic liver disease. AIH’s prevalence varies and has increased recently. Diagnosis involves the discovery of histologic features following liver biopsy and serologic testing. Clinical features vary, and up to 40% of patients may be asymptomatic. Evaluating thiopurine methyltransferase (TMPM) activity before treatment is crucial for an optimal response. The primary treatment goal is biochemical remission, normalized serum IgG, and liver enzymes. Induction therapy typically involves azathioprine and corticosteroids. Close monitoring of liver function tests and serum immunoglobulin levels is essential. Medications can be tapered after achieving biochemical remission. Liver transplantation may be required for refractory disease or cirrhosis. Further therapeutic approaches are needed, particularly for non-responders to first-line treatments.
Test Method for Rapid Prediction of Steady-State Temperature of Outer Rings of Bearings under Grease Lubrication Conditions
Authors: Xia Zhongbing , Yang Fang , Ma Xiqiang , Guo Nan , Wang Xiao , Cui Yunhao , Duan Yuchen
Published: May 2024

Temperature has a great influence on the stability of bearing performance. For the study of bearing steady-state temperature, this paper proposes a test method to quickly predict the steady-state temperature of the outer ring of a bearing, which solves the problems in traditional theoretical calculations and simulation analysis methods such as the large number of calculations, complex models, and large errors. Firstly, a mathematical prediction model is established according to the bearing temperature-rise law; then, a bearing steady-state temperature detection device is designed; and finally, the prediction model parameters are solved according to the experimental results, and experimental verification is carried out. It is shown that the prediction model has high accuracy under different load and speed conditions, and the error between the predicted steady-state temperature and the tested steady-state temperature is less than 0.7 °C. This prediction method reduces the single test time of the same speed to 60 min, which greatly improves the efficiency of the temperature detection test. The steady-state temperature model has important theoretical significance in guiding the study of the limiting speed of bearings.
Electromagnetic Characterization of Permanent Magnet Eddy Current Structures Based on Backplane Distance Adjustment
Authors: Wu Yipeng , Wang Teng , Song Tao , Guo Wenxiao
Published: May 2024

To address the problem of problematic spray design inside mining anchor-digging equipment, a switching seal using a permanent magnet eddy current drive is initially presented here. The layer model of the permanent magnet eddy current structure is established, the subdomain analysis model is introduced, the permanent magnet eddy current structure is divided into six regions along the axial direction, and the boundary equations are established at the interfaces of each region. The vector magnetic potential equations in each region are deduced, along with the electromagnetic torque and axial force equations. The computational results are compared and analyzed with the results of finite element simulation, verifying the accuracy of the theoretical model. The design of experiments is used to verify the feasibility of the switching seal using the permanent magnet eddy current structure.
Determination of Mechanical Power Loss of the Output Mechanisms with Serially Arranged Rollers in Cycloidal Gears While Taking into Account Manufacturing Tolerances
Authors: Antoniak Piotr , Bednarczyk Sławomir
Published: May 2024

Despite their complex design, cycloidal gearboxes are characterized by high efficiency. Nevertheless, due to friction, some power is lost during gearbox operation. Basically, these losses occur in two structural nodes: the cycloid gearing and the output mechanism. Since the first of these nodes has been well discussed in the literature, the output mechanism will be discussed in this article. The design of the output mechanism has a significant impact on mechanical power losses. There are several mechanism design solutions. One of them is a mechanism with serially arranged rollers. Three solutions that are different in design but work identically will be discussed. Due to this affinity, a single, common mathematical model will be used to determine the value of losses. As will be shown, the value of losses is directly affected by the backlash, number, and diameter of the rollers used in the output mechanism and indirectly by the ratio and eccentricity of the cycloidal gearbox. Sample calculations were carried out using the developed model of mechanical power losses in the output mechanism. This made it possible to analyze the distribution of backlash created by manufacturing tolerances. It was also shown that the backlash has a significant effect on the number of rollers involved in torque transmission, as well as on the distribution of loads, contact pressures, and mechanical power losses.
Tuning Structure and Properties of a Ferromagnetic Organic Semiconductor via a Magnetic Field-Modified Reduction Process
Authors: Zhou Han , Cheng Zaitian , Ai Zhiqiang , Li Xinyao , Hu Lin , Zhang Fapei
Published: May 2024

The development of novel synthesis and assembly strategies is critical to achieving a ferromagnetic organic semiconductor with high Curie temperature. In this study, we report a high magnetic field (HMF)-modified solvothermal approach for the reduction in neutral perylene diimide (PDI) into the dianion species to prepare the PDI magnets comprising radical anions after subsequent oxidation processes. The PDI materials, assembled from the dianion solution by an HMF-modified reduction, exhibit a smaller crystallite size and an enlarged distance of the π-π stacking in the PDI aggregates. Furthermore, the PDI magnets obtained from the process under a 9T field reveal weakened ferromagnetism and the rapid degradation of electrical conductivity compared to those prepared without a magnetic field. Based on spectral and structural characterizations, such performance deterioration originates from the enhanced instability of the radical anions exposed to air, as well as the decreased crystallinity for the radical PDIs synthesized from the HMF-modified reduction process. This work demonstrates that magnetic fields offer an effective way in the material synthesis process to manipulate the structure and magnetic properties of the radical-based organic magnets.
Model Recalibration for Regional Bias Reduction in Dynamic Microsimulations
Authors: Weymeirsch Jan , Ernst Julian , Münnich Ralf
Published: May 2024

Dynamic microsimulations are tools to stochastically project (synthetic) microdata into the future. In spatial microsimulations, regional discrepancies are of particular interest and must be considered accordingly. In practice, the probabilities for state changes are unknown and must be estimated, usually from survey data. However, estimating such models on the regional level is often not feasible due to limited sample size and lack of geographic information. Simply applying the model estimated at the national level to all geographies leads to biased state transitions due to regional differences in level and distribution. In this paper, we introduce a model-based alignment method to adapt predicted probabilities obtained from a nationally estimated model to subregions by integrating known marginal distributions to re-introduce regional heterogeneity and create more realistic trajectories, particularly in small areas. We show that the model-adjusted transition probabilities can capture region-specific patterns and lead to improved projections. Our findings are useful to researchers who want to harmonise model outputs with external information, in particular for the field of microsimulation.
Circulating Anti-Endothelial Cell Antibodies in Patients with Geographic Atrophy Related to Dry Age-Related Macular Degeneration
Authors: Żuber-Łaskawiec Katarzyna , Wilańska Joanna , Karska-Basta Izabella , Pociej-Marciak Weronika , Romanowska-Dixon Bożena , Sanak Marek , Kubicka-Trząska Agnieszka
Published: May 2024

Background and Objectives: Age-related macular degeneration (AMD) is one of the leading causes of central vision loss among elderly patients, and its dry form accounts for the majority of cases. Although several causes and mechanisms for the development and progression of AMD have previously been identified, the pathogenesis of this complex disease is still not entirely understood. As inflammation and immune system involvement are strongly suggested to play a central role in promoting the degenerative process and stimulating the onset of complications, we aimed to analyze the frequency of serum anti-retinal (ARAs) and anti-endothelial cell antibodies (AECAs) in patients with dry AMD and to determine their relationship with the clinical features of the disease, notably the area of geographic atrophy (GA). Materials and Methods: This study included 41 patients with advanced-stage dry AMD and 50 healthy controls without AMD, matched for gender and age. ARAs were detected by indirect immunofluorescence using monkey retina as an antigen substrate, and the presence of AECAs was determined using cultivated human umbilical vein endothelial cells and primate skeletal muscle. Results: ARAs were detected in 36 (87.8%) AMD patients (titers ranged from 1:20 to 1:320) and in 16 (39.0%) (titers ranged from 1:10 to 1:40) controls (p = 0.0000). Twenty of the forty-one patients (48.8%) were positive for AECAs, while in the control group, AECAs were present only in five sera (10.0%). The titers of AECAs in AMD patients ranged from 1:100 to 1:1000, and in the control group, the AECA titers were 1:100 (p = 0.0001). There were no significant correlations between the presence of AECAs and disease activity. Conclusions: This study demonstrates a higher prevalence of circulating AECAs in patients with dry AMD; however, no correlation was found between the serum levels of these autoantibodies and the area of GA.
Major Bleeding Events in Hospitalized COVID-19 Patients: A Retrospective Observational Study
Authors: Poloni Andrea , Casalini Giacomo , Pozza Giacomo , Giacomelli Andrea , Colaneri Marta , Carrozzo Giorgia , Caloni Beatrice , Ciubotariu Cosmin Lucian , Zacheo Martina , Rabbione Andrea , Pieruzzi Margherita , Barone Federico , Passerini Matteo , Ridolfo Anna Lisa , Rizzardini Giuliano , Gori Andrea , Antinori Spinello
Published: May 2024

Thromboprophylaxis/anticoagulation treatment is often required in hospitalized COVID-19 patients. We aimed to estimate the prevalence of major bleeding events in hospitalized COVID-19 patients. This was a retrospective observational study including all COVID-19 hospitalized patients ≥18 years of age at one reference center in northern Italy. The crude prevalence (between February 2020–2022) of major bleeding events was estimated as the number of major bleeding episodes divided by patients at risk. Uni- and multivariable Cox models were built to assess factors potentially associated with major bleeding events. Twenty-nine (0.98%) out of 2,945 COVID-19 patients experienced a major bleeding event [prevalence of 0.55% (95%CI 0.37–0.79)], of which five were fatal. Patients who experienced a major bleeding event were older [78 years (72–84 IQR) vs. 67 years (55–78 IQR), p-value < 0.001] and more frequently exposed to anti-aggregating therapy (44.8% vs. 20.0%, p-value 0.002) when compared to those who did not. In the multivariable Cox model, age [per 1 year more AHR 1.05 (CI95% 1.02–1.09)] was independently associated with an increased risk of major bleeding events. A strict monitoring of older hospitalized COVID-19 patients is warranted due to the risk of major bleeding events.
Laser Forced Dehydration of Benign Vascular Lesions of the Oral Cavity: A Valid Alternative to Surgical Techniques
Authors: Gobbo Margherita , Guarda-Nardini Luca
Published: May 2024

Background and Objectives: Low-flow vascular lesions are commonly encountered in the oral cavity and may require removal due to aesthetic concerns, repeated bleeding or a cluttering sensation. Laser devices represent an excellent aid due to their affinity with blood and to their biostimulating properties and have been substituting traditional excision in selected cases. Materials and Methods: In this study, 30 patients presenting with low-flow oral vascular lesions were included. The lesions were clinically evaluated as follows: lesion’s site, reason for treatment, lesion’s dimensions, confirmation of positive diascopy via compression with a glass slide and photograph. The lesions were treated with laser forced dehydration (LFD) and then followed-up after 3 weeks, 6 months and 1 year. The laser source was a K-Laser Blu Derma (Eltech, K-Laser S.r.l., Via Castagnole, 20/H, Treviso, Italy). In the case of incomplete healing, a further protocol was performed at the three-week follow-up, and a further follow-up was scheduled for three weeks after. The following aspects were evaluated at each appointment: pain, using a Numeric Rating Scale (NRS) from 0 to 10 (0 = no pain, 10 = worst pain ever); the need to take painkillers (day of intervention and during follow-up); bleeding (yes/no); scar formation. Results: Complete regression was obtained in all patients, with no side effects. Only one patient required a second LFD protocol. NRS was 0 for all patients for the whole duration of the follow-up. None of the patients took painkillers on the day of the intervention and during the follow-up. One patient declared slight bleeding the day of the intervention, which she easily managed at home. One patient showed a small non-retracting and non-painful scar at the three-week follow-up. No recurrences were found after six months and one year. Conclusions: LFD targets endogenous chromophores, minimizing damage to adjacent tissue and limiting side effects. LFD is effective and could be considered a conservative alternative to traditional excision in low-flow lesions.
Colliding Challenges: An Analysis of SARS-CoV-2 Infection in Patients with Pulmonary Tuberculosis versus SARS-CoV-2 Infection Alone
Authors: Mihuta Camil , Socaci Adriana , Hogea Patricia , Tudorache Emanuela , Mihuta Monica Simina , Oancea Cristian
Published: May 2024

Background and Objectives: The concurrent occurrence of tuberculosis and COVID-19 coinfection poses significant clinical complexities, warranting a nuanced approach to diagnosis, management, and patient care. Materials and Methods: A retrospective, cross-sectional study was conducted on two groups: one comprising 32 patients with pulmonary TB (PTB) and COVID-19 co-infection, and one including 100 patients with COVID-19 alone. Data was collected from medical records, including patient history, clinical parameters, laboratory, imaging results, and patient outcome. Results: A lower BMI emerges as a significant marker suggesting underlying PTB in patients with SARS-CoV-2 co-infection. Type 2 diabetes mellitus increases the risk of death in PTB-SARS-CoV-2 co-infection. Co-infected patients show lymphocytopenia and higher neutrophil levels, CRP, transaminases, and D-dimer levels. Elevated CRP and ALT levels are linked to increased co-infection likelihood. Certain parameters like SpO2, CRP, ALT, AST, and D-dimer effectively differentiate between co-infected and COVID-19 patients. Platelet-to-lymphocyte ratio is notably higher in co-infected individuals. Lesion severity on imaging is significantly associated with co-infection, highlighting imaging’s diagnostic importance. Longer hospital stays are linked to co-infection but not significantly to death risk. Conclusions: Certain clinical and biological factors may serve as potential indicators of PTB co-infection in patients with SARS-CoV-2.
Effects of Strain Rate on the GND Characteristics of Deformed Polycrystalline Pure Copper
Authors: Ma Yidan , Liu Guisen , Yang Shuqing , Chen Ran , Xu Shuopeng , Shen Yao
Published: May 2024

Geometrically necessary dislocations (GNDs) play a pivotal role in polycrystalline plastic deformation, with their characteristics notably affected by strain rate and other factors, but the underlying mechanisms are not well understood yet. We investigate GND characteristics in pure copper polycrystals subjected to tensile deformation at varying strain rates (0.001 s−1, 800 s−1, 1500 s−1, 2500 s−1). EBSD analysis reveals a non-linear increase in global GND density with the strain rate rising, and a similar trend is also observed for local GND densities near the grain boundaries and that in the grain interiors. Furthermore, GND density decreases from the grain boundaries towards the grain interiors and this decline slows down at high strain rates. The origin of these trends is revealed by the connections between the GND characteristics and the behaviors of relevant microstructural components. The increase in grain boundary misorientations at higher strain rates promotes the increase of GND density near the grain boundaries. The denser distribution of dislocation cells, observed previously at high strain rates, is presumed to increase the GND density in the grain interiors and may also contribute to the slower decline in GND density near the grain boundaries. Additionally, grain refinement by higher strain rates also promotes the increase in total GND density. Further, the non-linear variation with respect to the strain rate, as well as the saturation at high strain rates, for grain boundary misorientations and grain sizes align well with the non-linear trend of GND density, consolidating the intimate connections between the characteristics of GNDs and the behaviors of these microstructure components.
Predictive Modelling and Optimization of the Mechanical Properties of Laser-Coated NB/SiC/Ni Welds Using an ANFIS
Authors: Zou Zhe , Chen Juan , Jean Ming-Der
Published: May 2024

In the present work, predictive modelling and optimization with the adaptive network based fuzzy inference system (ANFIS) modelling of the mechanical properties of laser-coated NB/SiC/Ni welds was studied based on the Taguchi design by laser cladding. An ANFIS model based on a Sugeno type fuzzy inference system was developed for predicting the hardness properties of SiC/BN/Ni welds by laser cladding with experimental data required for network training and prediction. Based on analysis of variance, three important factors were taken as inputs for the fuzzy logic inferences, while the hardness properties were taken as the output of the ANFIS. The microstructure of welds was analysed using scanning electron microscopy with an energy-dispersive X-Ray spectrometer. Highly developed leaf-like dendrites and eutectic crystals were found in some areas of the melting zone for the BN/SiC/Ni weld, which was significantly hardened. The ANFIS model based on Taguchi’s design provides a better pattern of response because the predicted and experimental values were highly similar. As a result, a satisfactory result was achieved between the predicted and experimental values of hardness in laser-coated NB/SiC/Ni welds, whereby the success and validity of the method was verified.
Endophytic Fungal Diversity in Hardwickia binata: Bridging the Gap between Traditional and Modern Techniques
Authors: Joe Xavier Sneha Michael , Thangavel Myithili , Mani Israel , Rajapriya Pandy , Ponnuraj Nagendraprabhu , Pandi Mohan
Published: May 2024

Endophytic fungus is crucial for maintaining plant health and defense mechanisms, acting as protective barriers against pathogens, and producing medicinally beneficial bioactive compounds. Genome sequencing and metagenomics have significantly enhanced the understanding of fungal diversity and metabolic capabilities, enabling the identification of new genes and substances. Traditional culture-dependent methods have been complemented by culture-independent techniques, offering a more comprehensive view of fungal diversity. Using both culture-dependent and culture-independent techniques, the present research investigation explored the diversity of endophytic fungi encountered in the foliage of Hardwickia binata. The study examined the topographical characteristics and nutritional content of soil samples collected from the locality of the selected plant sample, H. binata, to better comprehend the effects on the plant’s growth. The balanced nutrient constituted approximately a pH of 7.2, which suggested an alkaline nature and promoted plant development. The ratio of nitrogen, phosphorous, and potassium remained 3:1:1. A total of 25 fungal isolates, categorized into 17 morphotypes, were obtained using the culture-dependent approach; Curvularia and Nigrospora emerged as the most common genera. Furthermore, the prediction of the ITS2 secondary structure supports the identification of species, highlighting a wide variety of fungal species present in H. binata. The culture-independent approach generated 69,570 high-quality sequences, identifying 269 Operational Taxonomic Units (OTUs). The dominant Ascomycota phylum, along with various genera, indicated a rich fungal community associated with H. binata. This study advances the understanding of the endophytic fungus communities that are associated with H. binata and the nature of soil ecology. The findings emphasize the significance of holistic techniques in the study of microbial dynamics within plant systems as well as their implications for ecosystem management and plant health.
Bioaerosol Sampling Devices and Pretreatment for Bacterial Characterization: Theoretical Differences and a Field Experience in a Wastewater Treatment Plant
Authors: Gaetano Anastasia Serena , Semeraro Sabrina , Greco Samuele , Greco Enrico , Cain Andrea , Perrone Maria Grazia , Pallavicini Alberto , Licen Sabina , Fornasaro Stefano , Barbieri Pierluigi
Published: May 2024

Studies on bioaerosol bacterial biodiversity have relevance in both ecological and health contexts, and molecular methods, such as 16S rRNA gene-based barcoded sequencing, provide efficient tools for the analysis of airborne bacterial communities. Standardized methods for sampling and analysis of bioaerosol DNA are lacking, thus hampering the comparison of results from studies implementing different devices and procedures. Three samplers that use gelatin filtration, swirling aerosol collection, and condensation growth tubes for collecting bioaerosol at an aeration tank of a wastewater treatment plant in Trieste (Italy) were used to determine the bacterial biodiversity. Wastewater samples were collected directly from the untreated sewage to obtain a true representation of the microbiological community present in the plant. Different samplers and collection media provide an indication of the different grades of biodiversity, with condensation growth tubes and DNA/RNA shieldTM capturing the richer bacterial genera. Overall, in terms of relative abundance, the air samples have a lower number of bacterial genera (64 OTUs) than the wastewater ones (75 OTUs). Using the metabarcoding approach to aerosol samples, we provide the first preliminary step toward the understanding of a significant diversity between different air sampling systems, enabling the scientific community to orient research towards the most informative sampling strategy.
The Effect of Low HBV-DNA Viral Load on Recurrence in Hepatocellular Carcinoma Patients Who Underwent Primary Locoregional Treatment and the Development of a Nomogram Prediction Model
Authors: Xiong Yiqi , Wang Ziling , Liu Jiajun , Li Kang , Zhang Yonghong
Published: May 2024

(1) Background: HBV-DNA is an essential clinical indicator of primary hepatocellular carcinoma (HCC) prognosis. Our study aimed to investigate the prognostic implication of a low load of HBV-DNA in HCC patients who underwent local treatment. Additionally, we developed and validated a nomogram to predict the recurrence of patients with low (20–100 IU/mL) viral loads (L-VL). (2) Methods: A total of 475 HBV-HCC patients were enrolled, including 403 L-VL patients and 72 patients with very low (<20 IU/mL) viral loads (VL-VL). L-VL HCC patients were randomly divided into a training set (N = 282) and a validation set (N = 121) at a ratio of 7:3. Utilizing the Lasso–Cox regression analysis, we identified independent risk factors for constructing a nomogram. (3) Results: L-VL patients had significantly shorter RFS than VL-VL patients (38.2 m vs. 23.4 m, p = 0.024). The content of the nomogram included gender, BCLC stage, Glob, and MLR. The C-index (0.682 vs. 0.609); 1-, 3-, and 5-year AUCs (0.729, 0.784, and 0.783, vs. 0.631, 0.634, the 0.665); calibration curves; and decision curve analysis (DCA) curves of the training and validation cohorts proved the excellent predictive performance of the nomogram. There was a statistically significant difference in RFS between the low-, immediate-, and high-risk groups both in the training and validation cohorts (p < 0.001); (4) Conclusions: Patients with L-VL had a worse prognosis. The nomogram developed and validated in this study has the advantage of predicting patients with L-VL.