bluuebunny/crossref_metadata_2025 huggingface dataset:
Fantasías primitivas que regresan. Lo siniestro de La mujer desnuda (1950), de Armonía Somers
Authors: González Carina
Published: March 2022

Comprendida dentro de la zona de lo reprimido, la sexualidad ocupa un territorio tenso entre lo prohibido y la transgresión. Las representaciones que adopta son producto también de esa tensión y generan un desequilibrio que afecta no solo a la construcción de la subjetividad, sino a la sociedad que se estructura sobre esa represión. En este trabajo se abordará la aparición del sexo como disparador de la materia inconsciente, según los primeros textos psicoanalíticos de Freud, y se estudiará la operatividad de lo siniestro en una plataforma estética que vincula la novela de Armonía Somers al surrealismo, desplegando estrategias comunes en torno al automatismo, la desintegración de la identidad y la singularización de la escritura.
Carlos Rojas González (Guayaquil, 1941-2019): teorizar y pensar desde la escritura poética
Authors: Báez Meza Marcelo
Published: March 2022

Recorrido por la producción textual de una de las voces más sólidas de la poesía ecuatoriana. Un vistazo a cada uno de los libros que el guayaquileño Carlos Rojas González publicó desde el año 1990. De los pocos escritores que combinó las ciencias del lenguaje con la praxis poética. Su obra se puede resumir como la semiótica de una doble pasión: la de la mujer y la ciudad amada. La pulcritud de su fraseo y la riqueza conceptual son dos de los tantos aportes de un semiólogo que tensionó al máximo el discurso poético.
Desbordar la escritura: Azulinaciones, deconstrucción de la novela pulsional
Authors: Chamorro Diego
Published: March 2022

La novela Azulinaciones (1990) de Natasha Salguero constituye un importante referente de experimentación con el lenguaje, descentramiento de la literatura canónica y afirmación de una escritura femenina del exceso y el desborde. Esta escritura fragmentaria se presenta desde categorías críticas como différance y diseminación, tomadas de la teoría de la deconstrucción de Jacques Derrida. Con estos elementos se puede aproximar una lectura desde los márgenes del mundo literario, donde las perspectivas toman otros lugares en la interpretación y las maniobras de escritura en la novela de Salguero, configurando una estética de lo cotidiano. Además, se rescata la importancia de la relación entre la lengua y el transitar un espacio, para convertirse el espacio ficcional en un espacio vivo y en movimiento. En definitiva, Azulinaciones se convierte en una muestra de la “novela pulsional” que se desmarca de las jerarquías racionalizantes y caducas de la literatura, más bien propone un juego único en cuanto al arte de la escritura y la deconstrucción de las formas literarias.
The Role of Culture in the Organization
Authors: Sarwari Guldasta
Published: March 2022

The leading aim of this article is an investigation of the role of culture in the organization. Organizational culture represents values, beliefs, hypotheses, fables, norms, and goals, which are admitted in the organization vastly. The organizational culture is the means which facilitates our behavior and actions with organizational tasks. The findings of this study reveal that one of the most influential factors in an organization’s situation is organizational culture. In the current complicated and revolutionary world, the durability of organizations is related to various factors; one of the most important factors is having a fortified organizational culture. The organizational culture is the subordinate outcome of social culture that, by its turn, impacts on values, moralities, attitudes, assumptions, and expectations of any individuals and can be exposed in their behaviors. The organizational culture has become more significant and remarkable because of its high and noticeable effects in organizations. Realizing the organizational culture is one of the causes that impact the real actions of people and their perceptions regarding that. The organizational culture represents the mode of our interpretation of our surrounding incidents. The research method of this study is library-based, and the information and points were collected from books and journals.
The risk of connectivity and finding God in the network: Analyzing Serial Experiments Lain through a critical surveillance lens
Authors: Anzivino Sophie
Published: March 2022

The 1998 anime Serial Experiments Lain presents a philosophical overload as it examines the violent and violating consequences of an overreliance on technology inflicted upon individuals and society. Exploring a society in which communications are structured by the Wired, a far-reaching network that integrates all aspects of communications and entertainment media, characters in the anime turn to technology in various ways to attempt to solve their social and emotional issues. Their issues do not dissipate, but rather their privacy and autonomy. Bringing Serial Experiments Lain into conversation with Foucault’s Birth of the Prison and Ian Hacking’s The Taming of Chance, this paper examines the continuity between the religious worshipping of gods and the fervorous belief in technology as sources of predictability, stability, and connection to something bigger than themselves. As the anime, Foucault, and Hacking warn, the consequences of such attitudes to privacy and autonomy are significant.
Artificial intelligence and global governance: How AI ethics and standards should be addressed at the global level
Authors: Naidoo Mishka
Published: March 2022

There have been huge leaps in artificial intelligence (AI) technologies in recent years, which have led to remarkable advancements from self-driving cars to opening your phone with Face ID. Without algorithms and other AI-based technologies, many sectors in society would not exist—they would still be immersed in sci-fi fantasies. But what is artificial intelligence? Is it how sci-fi media predicted it to be, a world filled with Terminators or a future like the Matrix? Or is it something more romantic and subtle, like the recent AI movie Her? The word itself has many different connotations, and this multiplicity reflects the lack of consensus around how people view AI and its intentions. Regardless of how AI is conceptualized, consideration must be given to its governance. This article works toward establishing a starting point for understanding AI global governance models.
PERGELARAN VICOLMING WAYANG BEBER 4 KOTA SEBAGAI SOLUSI DAMPAK COVID-19
Authors: Sugihartono Ranang Agung , Rianto Jaka , Marwati Sri
Published: March 2022

The Covid-19 pandemic changed all the aspects of life, including the arts. Traditional artists were badly affected. They do not have any requests for art performances at all, because the performances will definitely gather the masses, including the beber puppet (wayang beber). For that, it is necessary to find a solution to overcome this condition. Through this artistic research, the researcher created a model for presenting the beber wayang show using multi-platform technology, namely the integration of video conferencing and live streaming, abbreviated as Vicolming. This artistic research uses a type of practice-based research with a ‘Multi-Method’ approach with stages including information gathering, selection, preparation, analysis, evaluation, presentation, and communication. The results show that the wayang beber show performed with multi-platform technology can produce offerings or performance that are not only online but interesting, creative, interactive, collaborative, and can be watched from anywhere live and on demand. The results of this research are expected to become an online performance model that can be applied to other traditional performing arts.Keywords: Vicolming, multi-platform, wayang beber, and Covid-19
PENCIPTAAN KARYA-KARYA SENI CETAK SARING DENGAN OBJEK VISUAL YANG BERBASIS KEARIFAN LOKAL
Authors: Adi Sigit Purnomo , Sukerta Pande Made , Marianto Martinus Dwi , Hadi Sri
Published: March 2022

Sablon or screen printing has many functions and is usually more popular in the community for making t-shirts and banners and even batik cloth. The popularity of screen printing or screen printing, which is used by world-class artists, one of them Andy Warhol and also used by Marida Nasution, causes writers to feel inspired to work using this technique, because the technique is already popular in the community, making it easy to get tools and materials. To visualize this work, the author uses an art creation research methodology with an artistic creation process. The artistic creation process requires several stages to be passed, including experimentation, reflection and finally formation. The selection of these objects is indeed very interesting because these objects are increasingly disappearing in our lives. So that in this work, we try to marry or collaborate between modern art represented by graphic art with visual objects based on local wisdom that represent tradition. The paradigm of modern art with a touch of tradition as an effort to find the roots of the characteristics of Indonesia from year to year has begun to be explored by artists in Indonesia as an effort to find Indonesian cultural identity.Keyword: Sablon, Artistic Creative, Printmaking.
Female erectile tissues and sexual dysfunction after pelvic radiotherapy: A scoping review
Authors: Marshall Deborah C. , Tarras Elizabeth S. , Ali Ayesha , Bloom Julie , Torres Mylin A. , Kahn Jenna M.
Published: March 2022

Sexual function is a vital aspect of human health and is recognized as a critical component of cancer survivorship. Understanding and evaluating the impacts of radiotherapy on female sexual function requires precise knowledge of the organs involved in sexual function and the relationship between radiotherapy exposure and sexual tissue function. Although substantial evidence exists describing the impact of radiotherapy on male erectile tissues and related clinical sexual outcomes, there is very little research in this area in females. The lack of biomedical data in female patients makes it difficult to design studies aimed at optimizing sexual function postradiotherapy for female pelvic malignancies. This scoping review identifies and categorizes current research on the impacts of radiotherapy on normal female erectile tissues, including damage to normal functioning, clinical outcomes of radiation‐related female erectile tissue damage, and techniques to spare erectile tissues or therapies to treat such damage. An evaluation of the evidence was performed, and a summary of findings was generated according to Preferred Reporting Items for Systematic Review and Meta‐Analysis (PRISMA) Extension for Scoping Reviews guidelines. Articles were included in the review that involved normal female erectile tissues and radiotherapy side effects. The results show that little scientific investigation into the impacts of radiotherapy on female erectile tissues has been performed. Collaborative scientific investigations by clinical, basic, and behavioral scientists in oncology and radiotherapy are needed to generate radiobiologic and clinical evidence to advance prospective evaluation, prevention, and mitigation strategies that may improve sexual outcomes in female patients.
Analysis of Skills and Knowledge of Dalmas Personnel Case Study at Polres Cianjur
Authors: Anjar Maulana  , Basir S  , Firman Fadillah
Published: March 2022

Security and public order are important in running the government. The National Police of the Republic of Indonesia (POLRI) is mandated by Law Number 9 of 1998 concerning the freedom to express opinions in public, to organize security demonstrations by ensuring security and public order in accordance with applicable procedures. Demonstration by the Cianjur Police. Based on the results of the West Java Police Propam field examination contained in the Disciplinary Violation Preliminary Examination file number: DP3D-13/K/II/HUK.12.10/2020/Bid-Propam and the police report with the number: LP/6-A/I/HUK. 12.10/2020/Bid-Propam. This study uses a qualitative method with a single case approach, this research approach has a case dissection perspective by looking at aspects of personnel competence when securing demonstrations so that the expected approach will bring the best results. Cianjur Police personnel who have implemented Dikbangspes associated with competency theory have resulted in the view that 6 (six) Cianjur Police personnel do not have the expertise (Dikjur) and skills (short training) in the field of technical functions of the Sabhara Unit police. Then, there are three policy makers (Head of Ops, Kasat Binmas, and KBO Sabhara) during the process of securing demonstrations, have long service experience, namely Head of Ops KOMPOL Warsito has served for 33 years, Head of Binmas AKP Achmad Suprijatna has served for 30 years, and KBO Sabhara has served for 28 years.
The Impact of Multinationals on Labour Relations in Developing Countries: A Literature Review
Authors: Hassan Elsan Mansaray
Published: March 2022

The study discusses the impact of multinationals on labour relations in developing countries with special reference to Malaysia and Nigeria. The study discovered that several studies have been done on MNCs operating in Malaysia and revealed that the Malaysian government had used oppressive methods and strategies to keep trade unions in Malaysia dormant, so as to meet the urges of MNCs operating within the country. Malaysia has a history of no proper organized labour in the MNCs’ industries, because the managements of those industries are anti-union. Moreover, they just wanted to operate without any union interference, so that they can appropriate workers labour and maximized profit. For the case of Nigeria, there is organized and recognized labour but; their effort to protect members are sometimes dashed away by corrupt institutions that are empowered to monitor and evaluate MNCs activities. Bribery by MNCs to officials of these institutions have weakened the labour unions to carry out their mandate effectively. Hence, MNCs use the opportunity to override some labour laws like salary discrimination between expatriates and locals and ineffective human resource development. They usually fix higher salaries for their expatriates and low salaries for the local workers without being checked by the Nigerian authorities.
Pengaruh Bimbingan Kelompok Teknik Diskusi Dalam Meningkatkan Kemampuan Komunikasi Peserta Didik Di SMPN 1 Rasanae Barat Kota Bima
Authors: Khairunnisa Khairunnisa , Nurmaya Alya , Indah Sulistia , Nujumi Nurlailatul
Published: March 2022

Peserta didik yang tidak memiliki kemampuan komunikasi cenderung tidak fokus dan tidak mau menyampaikan pendapatnya karena kurang berani mengeluarkan isi pendapatnya karena takut salah dan ditertawakan oleh teman-temannya khususnya di SMPN 1 Rasanae Barat Kota Bima. Adapun yang menjadi subyek dalam penelitian ini adalah siswa di SMPN 1 Rasanae Barat Kota Bima sebanyak 30 orang peserta didik. Tehnik pengumpulan data pada penelitian ini adalah menggunakan tehnik angket, observasi dan dokumentasi. Adapun analisis data dalam penelitian ini menggunakan analisis t tes untuk mendeskripsikan pendapat responden terhadap bimbingan kelompok teknik diskusi dalam meningkatkan kemampuan komunikasi peserta didik. Berdasarkan paparan data dan hasil temuan ditarik kesimpulan sebagai berikut: 1) Pengaruh bimbingan kelompok teknik diskusi dalam meningkatkan kemampuan komunikasi peserta didik adalah sebesar 33,90 dan nilai I dikonsultasikan dengan nilai t tabel maka diperoleh nilai t tabel dengan taraf signifikansi 5% dan df = 29 maka diperoleh t tabel adalah 2,42. 2). Rata-rata tanggapan positif peserta didik dengan adanya pengaruh bimbingan kelompok teknik diskusi dalam meningkatkan kemampuan komunikasi peserta didik adalah sebesar 11,32 dan rata-rata tanggapan negatif siswa dengan adanya pengaruh bimbingan kelompok teknik diskusi dalam meningkatkan kemampuan komunikasi peserta didik adalah sebesar 3,77. 3) Dari 8 (delapan) aspek bimbingan kelompok yang diobservasi peneliti diperoleh 75% pada kriteria cukup dan 25% pada kriteria baik. Ini menandakan bahwa pengaruh bimbingan kelompok teknik diskusi dalam meningkatkan kemampuan komunikasi peserta didik di SMPN 1 Rasanae Barat Kota Bima dikategorikan cukup baik.
Instantaneous Frequency-Embedded Synchrosqueezing Transform for Signal Separation
Authors: Jiang Qingtang , Prater-Bennette Ashley , Suter Bruce W. , Zeyani Abdelbaset
Published: March 2022

The synchrosqueezing transform (SST) and its variants have been developed recently as an alternative to the empirical mode decomposition scheme to model a non-stationary signal as a superposition of amplitude- and frequency-modulated Fourier-like oscillatory modes. In particular, SST performs very well in estimating instantaneous frequencies (IFs) and separating the components of non-stationary multicomponent signals with slowly changing frequencies. However its performance is not desirable for signals having fast-changing frequencies. Two approaches have been proposed for this issue. One is to use the 2nd-order or high-order SST, and the other is to apply the instantaneous frequency-embedded SST (IFE-SST). For the SST or high order SST approach, one single phase transformation is applied to estimate the IFs of all components of a signal, which may yield not very accurate results in IF estimation and component recovery. IFE-SST uses an estimation of the IF of a targeted component to produce accurate IF estimation. The phase transformation of IFE-SST is associated with the targeted component. Hence the IFE-SST has certain advantages over SST in IF estimation and signal separation. In this article, we provide theoretical study on the instantaneous frequency-embedded short-time Fourier transform (IFE-STFT) and the associated SST, called IFE-FSST. We establish reconstructing properties of IFE-STFT with integrals involving the frequency variable only and provide reconstruction formula for individual components. We also consider the 2nd-order IFE-FSST.
Detecting Ultra- and Circadian Activity Rhythms of Dairy Cows in Automatic Milking Systems Using the Degree of Functional Coupling—A Pilot Study
Authors: Fuchs Patricia , Adrion Felix , Shafiullah Abu Z. M. , Bruckmaier Rupert M. , Umstätter Christina
Published: March 2022

Ultra- and circadian activity rhythms of animals can provide important insights into animal welfare. The consistency of behavioral patterns is characteristic of healthy organisms, while changes in the regularity of behavioral rhythms may indicate health and stress-related challenges. This pilot study aimed to examine whether dairy cows in free-stall barns with an automatic milking system (AMS) and free cow traffic can develop ultra- and circadian activity rhythms. On 4 dairy farms, pedometers recorded the activity of 10 cows each over 28 days. Based on time series calculation, the Degree of Functional Coupling (DFC) was used to determine the cows' activity rhythms. The DFC identified significant rhythmic patterns in sliding 7-day periods and indicated the percentage of activity (0–100%) that was synchronized with the 24-h day-night rhythm. As light is the main factor influencing the sleep-wake cycle of organisms, light intensity was recorded in the AMS, at the feed alley and in the barn of each farm. In addition, feeding and milking management were considered as part of the environmental context. Saliva samples of each cow were taken every 3 h for 1 day to determine the melatonin concentration. The DFC approach was successfully used to detect activity rhythms of dairy cows in commercial housing systems. However, large inter- and intra-individual variations were observed. Due to a high frequency of 0 and 100%, a median split was used to dichotomize into “low” (<72.34%) and “high” (≥72.34%) DFC. Forty percent of the sliding 7-day periods corresponded to a low DFC and 50% to a high DFC. No DFC could be calculated for 10% of the periods, as the cows' activity was not synchronized to 24 h. A generalized linear mixed-effects model revealed that the DFC levels were positively associated with a longer milking interval and a higher amount of daytime activity and negatively associated with higher number of lactations. The DFC is a novel approach to animal behavior monitoring. Due to its automation capability, it represents a promising tool in its further development for the purpose of longitudinal monitoring of animal welfare.
LEVERSC: Cross-Platform Scriptable Multichannel 3-D Visualization for Fluorescence Microscopy Images
Authors: Winter Mark , Cohen Andrew R.
Published: March 2022

We describe a new open-source program called LEVERSC to address the challenges of visualizing the multi-channel 3-D images prevalent in biological microscopy. LEVERSC uses a custom WebGL hardware-accelerated raycasting engine unique in its combination of rendering quality and performance, particularly for multi-channel data. Key features include platform independence, quantitative visualization through interactive voxel localization, and reproducible dynamic visualization via the scripting interface. LEVERSC is fully scriptable and interactive, and works with MATLAB, Python and Java/ImageJ.
Encapsulating IM7-Displaying Yeast Cells in Calcium Alginate Beads for One-Step Protein Purification and Multienzyme Biocatalysis
Authors: Yin Wenhao , Wang Xinping , Liao Ying , Ma Lixin , Qiao Jie , Liu Hui , Song Xin , Liu Yi
Published: March 2022

There are several commercial chromatographic systems for protein purification; however, development of cost-effective 3H-grade (high yield, high purity, and high activity) purification approaches is highly demanded. Here, we establish a methodology for encapsulating the IM7-displaying yeast cells in calcium alginate beads. Taking advantage of this biomaterial-based affinity chromatography, rapid and cost-effective purification of proteins with over 90% purity in a single step is achieved. Moreover, our system enables coating the multienzyme complex to produce reusable immobilized cells for efficient cascade biotransformation. Together, the present method has great application potentials not only in the laboratory but also in the industry for production of protein products as well as biocatalysis.
Identification of the Immune Signatures for Ovarian Cancer Based on the Tumor Immune Microenvironment Genes
Authors: Shen Xiaoyan , Gu Xiao , Ma Ruiqiong , Li Xiaoping , Wang Jianliu
Published: March 2022

Ovarian cancer (OV) is a deadly gynecological cancer. The tumor immune microenvironment (TIME) plays a pivotal role in OV development. However, the TIME of OV is not fully known. Therefore, we aimed to provide a comprehensive network of the TIME in OV. Gene expression data and clinical information from OV patients were obtained from the Cancer Genome Atlas Program (TCGA) database. Non-negative Matrix Factorization, NMFConsensus, and nearest template prediction algorithms were used to perform molecular clustering. The biological functions of differentially expressed genes (DEGs) were identified using Metascape, gene set enrichment analysis (GSEA), gene ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis. The copy number variations (CNVs), single nucleotide polymorphisms (SNPs) and tumor mutation burden were analyzed using Gistic 2.0, R package maftools, and TCGA mutations, respectively. Estimation of STromal and Immune cells in MAlignant Tumor tissues using Expression data and CIBERSORT were utilized to elucidate the TIME. Moreover, external data from the International Cancer Genome Consortium (ICGC) and ArrayExpress databases were used to validate the signature. All 361 samples from the TCGA OV dataset were classified into Immune Class and non-Immune Class with immune signatures. By comparing the two classes, we identified 740 DEGs that accumulated in immune-related, cancer-related, inflammation-related biological functions and pathways. There were significant differences in the CNVs between the Immune and non-Immune Classes. The Immune Class was further divided into immune-activated and immune-suppressed subtypes. There was no significant difference in the top 20 genes in somatic SNPs among the three groups. In addition, the immune-activated subtype had significantly increased proportions of CD4 memory resting T cells, T cells, M1 macrophages, and M2 macrophages than the other two groups. The qRT-PCR results indicated that the mRNA expression levels of RYR2, FAT3, MDN1 and RYR1 were significantly down-regulated in OV compared with normal tissues. Moreover, the signatures of the TIME were validated using ICGC cohort and the ArrayExpress cohort. Our study clustered the OV patients into an immune-activated subtype, immune-suppressed subtype, and non-Immune Class and provided potential clues for further research on the molecular mechanisms and immunotherapy strategies of OV.
Long Non-Coding RNAs in Cryptococcus neoformans: Insights Into Fungal Pathogenesis
Authors: Kalem Murat C. , Panepinto John C.
Published: March 2022

Long non-coding RNAs (lncRNAs) are highly expressed and can modulate multiple cellular processes including transcription, splicing, translation, and many diverse signaling events. LncRNAs can act as sponges for miRNAs, RNA and DNA binding proteins, functioning as competitive endogenous RNAs. The contribution of lncRNAs to microbial pathogenesis is largely neglected in eukaryotic pathogens despite the abundance of RNA sequencing datasets encompassing conditions of stress, gene deletions and conditions that mimic the host environment. The human fungal pathogen Cryptococcus neoformans encodes 6975 (84%) protein-coding and 1359 (16%) non-protein-coding RNAs, of which 1182 (14.2%) are lncRNAs defined by a threshold of greater than 200 nucleotides in length. Here, we discuss the current state of knowledge in C. neoformans lncRNA biology. Utilizing existing RNA seq datasets, we examine trends in lncRNA expression and discuss potential implications for pathogenesis.
Pathophysiologic Basis and Diagnostic Approaches for Ischemia With Non-obstructive Coronary Arteries: A Literature Review
Authors: Fu Bingqi , Wei Xuebiao , Lin Yingwen , Chen Jiyan , Yu Danqing
Published: March 2022

Ischemia with non-obstructive coronary arteries (INOCA) has gained increasing attention due to its high prevalence, atypical clinical presentations, difficult diagnostic procedures, and poor prognosis. There are two endotypes of INOCA—one is coronary microvascular dysfunction and the other is vasospastic angina. Diagnosis of INOCA lies in evaluating coronary flow reserve, microcirculatory resistance, and vasoreactivity, which is usually obtained via invasive coronary interventional techniques. Non-invasive diagnostic approaches such as echocardiography, single-photon emission computed tomography, cardiac positron emission tomography, and cardiac magnetic resonance imaging are also valuable for assessing coronary blood flow. Some new techniques (e.g., continuous thermodilution and angiography-derived quantitative flow reserve) have been investigated to assist the diagnosis of INOCA. In this review, we aimed to discuss the pathophysiologic basis and contemporary and novel diagnostic approaches for INOCA, to construct a better understanding of INOCA evaluation.
MRI Feature Tracking Strain in Pulmonary Hypertension: Utility of Combined Left Atrial Volumetric and Deformation Assessment in Distinguishing Post- From Pre-capillary Physiology
Authors: Leong Kai'En , Howard Luke , Lo Giudice Francesco , Pavey Holly , Davies Rachel , Haji Gulammehdi , Gibbs Simon , Gopalan Deepa
Published: March 2022

Aims

Pulmonary hypertension (PH) is dichotomized into pre- and post-capillary physiology by invasive catheterization. Imaging, particularly strain assessment, may aid in classification and be helpful with ambiguous hemodynamics. We sought to define cardiac MRI (CMR) feature tracking biatrial peak reservoir and biventricular peak systolic strain in pre- and post-capillary PH and examine the performance of peak left atrial strain in distinguishing the 2 groups compared to TTE.

Methods and Results

Retrospective cross-sectional study from 1 Jan 2015 to 31 Dec 2020; 48 patients (22 pre- and 26 post-capillary) were included with contemporaneous TTE, CMR and catheterization. Mean pulmonary artery pressures were higher in the pre-capillary cohort (55 ± 14 vs. 42 ± 9 mmHg; p < 0.001) as was pulmonary vascular resistance (median 11.7 vs. 3.7 WU; p < 0.001). Post-capillary patients had significantly larger left atria (60 ± 22 vs. 25 ± 9 ml/m2; p < 0.001). There was no difference in right atrial volumes between groups (60 ± 21 vs. 61 ± 29 ml/m2; p = 0.694), however peak RA strain was lower in post-capillary PH patients (8.9 ± 5.5 vs. 18.8 ± 7.0%; p < 0.001). In the post-capillary group, there was commensurately severe peak strain impairment in both atria (LA strain 9.0 ± 5.8%, RA strain 8.9 ± 5.5%). CMR LAVi and peak LA strain had a multivariate AUC of 0.98 (95% CI 0.89–1.00; p < 0.001) for post-capillary PH diagnosis which was superior to TTE.

Conclusion

CMR volumetric and deformation assessment of the left atrium can highly accurately distinguish post- from pre-capillary PH.
Identification of Potential Diagnostic Biomarkers From Circulating Cells During the Course of Sleep Deprivation-Related Myocardial Infarction Based on Bioinformatics Analyses
Authors: Chen Xiang , Li Qian , Zhang Zhong , Yang Minjing , Wang E.
Published: March 2022

Background

Myocardial infarction (MI) is the leading cause of death from non-infectious diseases worldwide and results in rapid deterioration due to the sudden rupture of plaques associated with atherosclerosis, a chronic inflammatory disease. Sleep is a key factor that regulates immune homeostasis of the body. The imbalance in circulating immune cells caused by sleep deprivation (SD) may represent a risk factor leading to the rapid deterioration of plaques and MI. Therefore, it is of profound significance to identify diagnostic biomarkers for preventing SD-related MI.

Methods

In the present study, we identified coexpressed differentially expressed genes (co-DEGs) between peripheral blood mononuclear cells from MI and SD samples (compared to controls) from a public database. LASSO regression analysis was applied to identify significant diagnostic biomarkers from co-DEGs. Moreover, receiver operating characteristic (ROC) curve analysis was performed to test biomarker accuracy and diagnostic ability. We further analyzed immune cell enrichment in MI and SD samples using the CIBERSORT algorithm, and the correlation between biomarkers and immune cell composition was assessed. We also investigated whether diagnostic biomarkers are involved in immune cell signaling pathways in SD-related MI processes.

Results

A total of 10 downregulated co-DEGs from the sets of MI-DEGs and SD-DEGs were overlapped. After applying LASSO regression analysis, SYTL2, KLRD1, and C12orf75 were selected and validated as diagnostic biomarkers using ROC analysis. Next, we found that resting NK cells were downregulated in both the MI samples and SD samples, which is similar to the changes noted for SYTL2. Importantly, SYTL2 was strongly positively correlated not only with resting NK cells but also with most genes related to NK cell markers in the MI and SD datasets. Moreover, SYTL2 was highly associated with genes in NK cell signaling pathways, including the MAPK signaling pathway, cytotoxic granule movement and exocytosis, and NK cell activation. Furthermore, GSEA and KEGG analyses provided evidence that the DEGs identified from MI samples with low vs. high SYTL2 expression exhibited a strong association with the regulation of the immune response and NK cell-mediated cytotoxicity.

Conclusion

In conclusion, SYTL2, KLRD1, and C12orf75 represent potential diagnostic biomarkers of MI. The association between SYTL2 and resting NK cells may be critically involved in SD-related MI development and occurrence.
The Prognostic Value of Right Atrial and Right Ventricular Functional Parameters in Systemic Sclerosis
Authors: Vos Jacqueline L. , Butcher Steele C. , Fortuni Federico , Galloo Xavier , Rodwell Laura , Vonk Madelon C. , Bax Jeroen J. , van Leuven Sander I. , de Vries-Bouwstra Jeska K. , Snoeren Miranda , El Messaoudi Saloua , Marsan Nina A. , Nijveldt Robin
Published: March 2022

Introduction

Right ventricular (RV) function is of particular importance in systemic sclerosis (SSc), since common SSc complications, such as interstitial lung disease and pulmonary hypertension may affect RV afterload. Cardiovascular magnetic resonance (CMR) is the gold standard for measuring RV function. CMR-derived RV and right atrial (RA) strain is a promising tool to detect subtle changes in RV function, and might have incremental value, however, prognostic data is lacking. Therefore, the aim of this study was to evaluate the prognostic value of RA and RV strain in SSc.

Methods

In this retrospective study, performed at two Dutch hospitals, consecutive SSc patients who underwent CMR were included. RV longitudinal strain (LS) and RA strain were measured. Unadjusted cox proportional hazard regression analysis and likelihood ratio tests were used to evaluate the association and incremental value of strain parameters with all-cause mortality.

Results

A total of 100 patients (median age 54 [46–64] years, 42% male) were included. Twenty-four patients (24%) died during a follow-up of 3.1 [1.8–5.2] years. RA reservoir [Hazard Ratio (HR) = 0.95, 95% CI 0.91–0.99, p = 0.009] and conduit strain (HR = 0.93, 95% CI 0.88–0.98, p = 0.008) were univariable predictors of all-cause mortality, while RV LS and RA booster strain were not. RA conduit strain proved to be of incremental value to sex, atrial fibrillation, NYHA class, RA maximum volume indexed, and late gadolinium enhancement (p < 0.05 for all).

Conclusion

RA reservoir and conduit strain are predictors of all-cause mortality in SSc patients, whereas RV LS is not. In addition, RA conduit strain showed incremental prognostic value to all evaluated clinical and imaging parameters. Therefore, RA conduit strain may be a useful prognostic marker in SSc patients.
A Review of Digital Health Strategies in 10 Countries With Young Populations: Do They Serve the Health and Wellbeing of Children and Youth in a Digital Age?
Authors: Holly Louise , Smith Robert Dean , Ndili Njide , Franz Christian , Stevens Enow Awah Georges
Published: March 2022

Children and youth merit special attention from digital health policymakers and practitioners because of the great potential for digital transformations to both enhance and undermine their health and wellbeing. However, an analysis of digital health strategies from 10 African countries with young populations suggest that national approaches to digital health are overlooking young people's specific health needs and unique risks in relation to digital technologies and data. To better serve the needs of children and youth in a digital age, future digital health strategies—and the global guidance that many strategies are based upon—should consider the ways in which digital transformations can positively or negatively impact the health and wellbeing of different populations, and the forms of cross-sectoral and multi-stakeholder collaboration required to amplify or mitigate them. Future strategies should be developed through inclusive processes that support young people's right to participate in decision-making that affects their lives.
Mechanism of Faster CH4 Bubble Growth Under Surface Waves in Muddy Aquatic Sediments: Effects of Wave Amplitude, Period, and Water Depth
Authors: Painuly Abhishek , Katsman Regina
Published: March 2022

Methane (CH4) transport from organic-rich fine-grained (muddy) shallow aquatic sediments to water column is mediated dominantly by discrete bubbles, which is an important natural source of greenhouse CH4. The lifespan of these bubbles within the sediment comprises two successive stages: growth from nucleation up to a mature size and then buoyant ascent toward the sediment–water interface. Bubbles often experience an oscillating overburden load due to the passage of winds and/or storm-induced short period surface waves or long-period tides, which can potentially affect both stages of the bubble’s lifespan. However, little is known about the wave effects over bubble growth phase. In the present work, this subject is investigated using a numerical single-bubble mechanical/solute transport model, which quantifies the effects of different parameters (amplitude and period) of the wave loading and of the water depth, over the bubble growth pattern in sediments and its specific characteristics. It was found that bubbles induce early sediment fracturing in the presence of waves, attributed to the low overburden load appearing at wave troughs. Bubbles at shallow depth rapidly grow at wave troughs by inducing multiple intense fracturing events. However, this ability decreases with an increasing water depth because of a slower solute influx. In the presence of waves, bubbles mature in shorter time, whose contrast to the no wave case is controlled by the ratio of wave amplitude to equilibrium water depth. Due to the higher frequency of occurrence of wave troughs for shorter-period waves, bubble growth is accelerated compared with the case of longer-period waves. Overall, our modeling suggests that the fastest bubble growth can be predicted for higher amplitude, short-period waves traveling in shallow water. We further infer that accelerated bubble growth, along with subsequent wave-induced ascent can sufficiently shorten the bubble’s total lifespan in sediment, which explains the observed episodic in situ ebullitions correlated with wind- or storm-induced waves.
Water-Rich Soft Soil Freezing Method Combined With Steel Sleeve Receiving-Shield Technology Field Test Research
Authors: Mei Y. , Liu Y. Y. , Yan X. , Liang C. , Zhu J. T.
Published: March 2022

The initiation and reception of shields are major risk events for shield construction in water-rich and weak strata. Although the freezing method and the steel sleeve method receiving-shield tunneling technologies both have engineering applications, the environmental safety control effect cannot meet the construction requirements of water-rich soft soil. Considering the shield construction of a typical soft soil layer in Suzhou, China, as a research target, the applicability and safety of the freezing method combined with steel sleeve receiving-shield technology in water-rich soft soil were evaluated based on a field test system. The test results show that, during soil freezing, the temperature change trend of each measuring point in the temperature measuring hole is roughly the same. The freezing process can be divided into five typical stages. The closer the active freezing period of the water-rich soft soil is to the inside of the frozen-soil curtain, the faster the development rate of the frozen wall. The soil cooling gradient increased with an increase in the radial depth. After freezing the curtain circle, the soil frost heave significantly accelerated until the frost heave amount peaked. During the construction process, special attention should be paid to the change in the value of the soil settlement during each stage change to prevent sudden changes in soil displacement. The freezing method, combined with steel sleeve receiving-shield technology, can effectively reduce the environmental disturbance caused by shield construction in water-rich soft soil.
Variability of Marine Methane Bubble Emissions on the Clayoquot Slope, Offshore Vancouver Island, Between 2017 and 2021
Authors: Marcon Yann , Römer Miriam , Scherwath Martin , Riedel Michael , Dølven Knut Ola , Heesemann Martin
Published: March 2022

Seabed methane gas emissions occur worldwide at cold seeps located along most continental margins. Fluxes of methane gas released from the seabed in the form of bubbles can be extremely variable even over short time intervals. Some factors controlling the variability are still poorly understood. Here, we report on the results of continuous long-term sonar monitoring of bubble emissions at a depth of 1,260 m on the Clayoquot Slope, northern Cascadia margin. With a total monitoring duration of 4 years and a sampling period of 1 h, this is by far the longest high temporal resolution monitoring of seabed methane gas release ever conducted. Our results provide evidence that the diurnal and semi-diurnal tides influence the timing of the onset and cessation of bubble emissions. However, gas emissions within the monitoring area are active more than 84% of the time, indicating that tides alone are not sufficient to make venting pause. We hypothesize that the gas fluxes are transient but generally sufficiently high to maintain ebullition independently of the tidally-induced bottom pressure variations. Results also show that the tides do not seem to modulate the vigor of active gas emissions.
Reinvigorating the Desire to Teach: Teacher Professional Development for Creativity, Agency, Stress Reduction, and Wellbeing
Authors: Anderson Ross C. , Katz-Buonincontro Jen , Livie Mari , Land Jessica , Beard Nathan , Bousselot Tracy , Schuhe Gabriella
Published: March 2022

Research suggests that teachers’ creative development may materialize in more resilience and joy and less stress, but these connections have received little attention. This mixed methods study analyzes the effectiveness of a hybrid professional development model focused on teachers’ creative agency during the COVID-19 pandemic, a period of intensified stress, anxiety, and disconnect. Results indicated the PD experience supported (a) an increase in teachers’ creative agency, empathy, joy, buoyancy, and support in teaching during the pandemic and (b) a reduction in their secondary traumatic stress. Qualitative analyses illustrated a variety of personalized pathways for this development. The evidence suggests teachers’ creative agency and wellbeing can develop through a complementary process, rooted in creativity and the arts.
Case Report: Neratinib Therapy Improves Glycemic Control in a Patient With Type 2 Diabetes and Breast Cancer
Authors: Angelis Vasileios , Johnston Stephen R. D. , Ardestani Amin , Maedler Kathrin
Published: March 2022

A critical decline of functional insulin-producing pancreatic β-cells is the central pathologic element of both type 1 and type 2 diabetes. Mammalian Sterile 20-like kinase 1 (MST1) is a key mediator of β-cell failure and the identification of neratinib as MST1 inhibitor with potent effects on β-cell survival represents a promising approach for causative diabetes therapy. Here we report a case of robust glycemia and HbA1c normalization in a patient with breast cancer-T2D comorbidity under neratinib, a potent triple kinase inhibitor of HER2/EGFR and MST1. The patient, aged 62 years, was enrolled in the plasmaMATCH clinical trial and received 240 mg neratinib once daily. Neratinib therapy correlated with great improvement in glucose and HbA1c both to physiological levels during the whole treatment period (average reduction of random glucose from 13.6 ± 0.4 to 6.3 ± 0.5 mmol/l and of HbA1c from 82.2 ± 3.9 to 45.6 ± 4.2 mmol/mol before and during neratinib). 18 months later, when neratinib was withdrawn, random glucose rapidly raised together with high blood glucose fluctuations, which reflected in elevated HbA1c levels. This clinical case reports the combination of HER2/EGFR/MST1-inhibition by neratinib for the pharmacological intervention to effectively restore normoglycemia in a patient with poorly controlled T2D and suggests neratinib as potent therapeutic regimen for the cancer-diabetes comorbidity.
Effect of Vitamin D Supplementation on In Vitro Fertilization Outcomes: A Trial Sequential Meta-Analysis of 5 Randomized Controlled Trials
Authors: Zhou Xiaoting , Wu Xiaomei , Luo Xi , Shao Jingyi , Guo Dongqun , Deng Bo , Wu Ze
Published: March 2022

Despite numerous studies indicating an imperative role of vitamin D for reproduction, the importance of vitamin D supplementation on in vitro fertilization (IVF) outcomes remains controversial. We therefore performed this meta-analysis to investigate the IVF outcomes of vitamin D supplementation in infertile women with vitamin D deficiency. We systematically searched PubMed, Embase and the Cochrane library for identifying all relevant studies published before August 2021. Pregnancy rate was defined as the primary outcome while good quality embryo, fertilization rate, ongoing pregnancy, and miscarriage were secondary outcomes. We used Review Manager 5.3 (RevMan) to conduct meta-analysis and examined the robustness of the primary outcome by trial sequential analysis. Five studies were included in the final analysis and it suggested that vitamin D supplementation was associated with improved chemical pregnancy rate (risk ratio [RR] = 1.53, 95% confidence interval [CI] = 1.06 to 2.20, p = 0.02) but not benefited in improving clinical pregnancy rate (RR = 1.34, 95% CI = 0.81 to 2.24, p = 0.25) and all secondary outcomes. Trial sequential analysis suggested further studies are needed to confirm this conclusion. We concluded that vitamin D supplementation should be prescribed to improve chemical pregnancy in infertile women with vitamin D deficiency and more studies are required to further confirm this finding.
The Changes of Irisin and Inflammatory Cytokines in the Age-Related Macular Degeneration and Retinal Vein Occlusion
Authors: Li Xiaochun , Cao Xiaoguang , Zhao Mingwei , Bao Yongzhen
Published: March 2022

Purpose

Age-related macular degeneration (AMD) and retinal vein occlusion (RVO) are irreversible chorioretinal diseases, which might induce severe damage in visual function. The metabolic factor and inflammatory factors might play important roles in the pathogenesis of AMD and RVO. The levels of irisin and 14 cytokines were analyzed in aqueous humor of AMD and RVO eyes to evaluate the roles of irisin and inflammatory factors.

Methods

We collected aqueous humor samples from patients with AMD (n = 27), RVO (n = 30), and cataract (as control, n = 23) eyes. Samples were assayed using ELISA kit for irisin and a multiplex immunoassay kit for 14 cytokines. The macular thickness (MT) was measured with OCT in all included eyes.

Results

MT in the RVO group is significantly higher than that in the AMD or control group. Irisin levels in the aqueous samples of AMD and RVO eyes were both significantly lower than that in the control. Furthermore, a positive correlation was found between irisin and MT in the RVO. Compared with the controls, AMD eyes had significantly higher levels of BDNF, VEGF-A, VEGF-R1, VEGF-R2, IL-10, TNF-α, VCAM-1, IP-10, and MCP-1. Similarly, RVO eyes had significantly higher levels of BDNF, VEGF-A, VEGF-R1, VEGF-R2, IL-6, IL-8, IL-10, TNF-α, ICAM-1, VCAM-1, IP-10, and MCP-1. However, there was no significant difference between the levels of PDGF-BB or TNF-β in these three groups. A negative correlation was found between VEGF-A and MT in AMD, as well as in control. Furthermore, a positive correlation was found between IL-6 and MT in the 80 included eyes, as well as in RVO. A positive correlation was found between ICAM-1 and MT in the 80 included eyes, as well as in RVO.

Conclusions

The metabolic factor, irisin levels in the aqueous humor are decreased in AMD and RVO eyes and show a positive correlation between irisin and MT in RVO eyes, prompting researchers to explore the relationship between irisin and macular edema. We also identified the higher expression of vascular growth factors (VEGF-A, VEGF-R1, and PDGF-BB), inflammatory cytokines (IL-6, IL-8, IL-10, and TNF-α), and chemokines (ICAM-1, VCAM-1, IP-10, and MCP-1) in AMD and RVO eyes.