bluuebunny/crossref_metadata_2025 huggingface dataset:
Development of a Real-Time PCR Method for the Detection of European and Siberian Subtypes of Tick-Borne Encephalitis Virus
Authors: Pedersen Benedikte N. , Jenkins Andrew , Paulsen Katrine M. , Basset Coraline , Andreassen Åshild K.
Published: October 2023

The tick-borne encephalitis virus (TBEV) is transmitted to humans through tick bites. In recent years, the appearance of the Siberian subtype of TBEV in Ixodes ricinus in Finland, together with deaths from the normally mild European subtype in the same country, have raised concerns about a possible spread of virulent variants of TBEV in Western Europe. Thus, there is a need to monitor the spread of strains, particularly of the European and Siberian subtypes. In this study, we develop a new real-time PCR method targeting Siberian and European subtypes of TBEV. The primers amplify a 176 bp fragment of the E gene, which is suitable for subsequent strain identification by Sanger sequencing. This study pioneers a new approach to primer design where the melting temperature (Tm) of primers annealed to representative mismatched target sequences is empirically determined and used to guide improvements in primer sequence. This allowed the range of TBEV strains detected to be extended to cover most European and Siberian strains tested, in addition to a strain of the Far-Eastern subtype. The limit of detection was 10–100 DNA copies per reaction and amplification efficiency varied between 83% and 94%, depending on the TBEV strain. Experimental determination of primer Tm proved to be a fruitful approach and will be a useful tool for future primer design and diagnostics.
The Equilibrium Molecular Structure of Cyclic (Alkyl)(Amino) Carbene Copper(I) Chloride via Gas-Phase Electron Diffraction and Quantum Chemical Calculations
Authors: Belyakov Alexander V. , Altova Ekaterina P. , Rykov Anatoliy N. , Sharanov Pavel Yu. , Shishkov Igor F. , Romanov Alexander S.
Published: October 2023

Copper-centered carbene–metal–halides (CMHs) with cyclic (alkyl)(amino) carbenes (CAACs) are bright phosphorescent emitters and key precursors in the synthesis of the highly promising class of the materials carbene–metal–amides (CMAs) operating via thermally activated delayed fluorescence (TADF). Aiming to reveal the molecular geometry for CMH phosphors in the absence of the intermolecular contacts, we report here the equilibrium molecular structure of the (CAAC)Cu(I)Cl (1) molecule in the gas-phase. We demonstrate that linear geometry around a copper atom shows no distortions in the ground state. The structure of complex 1 has been determined using the electron diffraction method, supported by quantum chemical calculations with RI-MP2/def2-QZVPP level of theory and compared with the crystal structure determined by X-ray diffraction analysis. Mean vibrational amplitudes, uij,h1, and anharmonic vibrational corrections (rij,e • rij,a) were calculated for experimental temperature T = 20 °C, using quadratic and cubic force constants, respectively. The quantum theory of atoms in molecules (QTAIM) and natural bond order (NBO) analysis of wave function at MN15/def2TZVP level of theory revealed two Cu…H, three H…H, and one three-center H…H…H bond paths with bond critical points. NBO analysis also revealed three-center, four-electron hyperbonds, (3c4e), [π(N–C) nπ(Cu) ↔ nπ(N) π(N–Cu)], or [N–C: Cu ↔ N: C–Cu] and nπ(Cu) → π(C–N)* hyperconjugation, that is the delocalization of the lone electron pair of Cu atom into the antibonding orbital of C–N bond.
Suppressing Dendrite Growth with Eco-Friendly Sodium Lignosulfonate Additive in Quasi-Solid-State Li Metal Battery
Authors: Tian Yingkang , Chen Xinyang , Gao Xuejie , Wu Hanyan , Cheng Chen , Cai Shuiping , Ren Wenfeng , Yang Xiaofei , Sun Runcang
Published: October 2023

The application of lithium metal batteries is limited by the drawbacks of safety problems and Li dendrite formation. Quasi-solid-state electrolytes (QSSEs) are the most promising alternatives to commercial liquid electrolytes due to their high safety and great compatibility with electrodes. However, Li dendrite formation and the slow Li+ diffusion in QSSEs severely hinder uniform Li deposition, thus leading to Li dendrite growth and short circuits. Herein, an eco-friendly and low-cost sodium lignosulfonate (LSS)-assisted PVDF-based QSSE is proposed to induce uniform Li deposition and inhibit Li dendrite growth. Li symmetric cells with 5%-LSS QSSE possess a high Li+ transfer number of 0.79, and they exhibit a long cycle life of 1000 h at a current density/areal capacity of 1 mA cm−2/5 mAh cm−2. Moreover, due to the fast electrochemical dynamics endowed by the improved compatibility of the electrodes and fast Li+ diffusion, the LFP/5%-LSS/Li full cells still maintain a high capacity of 110 mAh g−1 after 250 cycles at 6C. This work provides a novel and promising choice that uses eco-friendly LSS as an additive to PVDF-based QSSE in Li metal batteries.
Generation of a Recombinant scFv against Deoxycholic Acid and Its Conversion to a Quenchbody for One-Step Immunoassay
Authors: Ueda Hiroshi , Jeong Hee-Jin
Published: October 2023

Development of a rapid detection method for deoxycholic acid (DCA) is crucial for its diagnosis in the early stages of inflammation and cancer. In this study, we expressed a soluble recombinant anti-DCA single-chain variable fragment (scFv) in Escherichia coli. To convert scFv into a Quenchbody (Q-body), we labeled scFv using commercially available maleimide-linked fluorophores. The TAMRA-C5-maleimide-conjugated Q-body showed the highest response within a few minutes of DCA addition, indicating its applicability as a wash-free immunoassay probe for onsite DCA detection.
Diet and Depression during Peri- and Post-Menopause: A Scoping Review Protocol
Authors: Bodnaruc Alexandra M. , Duquet Miryam , Prud’homme Denis , Giroux Isabelle
Published: October 2023

The aim of the proposed scoping review is to describe and summarize studies assessing the associations between diet-related variables and depression in peri- and post-menopausal women. Studies examining the associations between diet-related variables and mental health indicators in women undergoing menopausal transition or in the post-menopausal period will be systematically retrieved via Medline, EMBASE, PsycINFO, Web of Science, and Scopus databases. All articles identified through the database searches will be imported into Covidence. Following the removal of duplicates, two authors will independently perform title and abstract screening, as well as full-text assessment against eligibility criteria. Data will be extracted using tables developed for observational and experimental studies. The methodological quality of randomized trials, cohort and cross-sectional studies, and case–control studies, will be assessed using the Cochrane risk-of-bias (RoB-2) tool, the NHLBI Quality Assessment Tool for Observational Cohort and Cross-Sectional Studies, and the NHLBI Quality Assessment Tool for Case–Control studies, respectively. Data extraction tables will be used to produce two tables summarizing the main characteristics and findings of the studies included in the review. In the proposed review, we will systematically identify and summarize the currently available evidence on the association between diet-related variables and depression in peri- and post-menopausal women. To our knowledge, this is the first review focusing on this subgroup of the population. Protocol registration: osf.io/b89r6.
Identifying Which Relational Cues Users Find Helpful to Allow Tailoring of e-Coach Dialogues
Authors: Salman Sana , Richards Deborah , Dras Mark
Published: October 2023

Relational cues are extracts from actual verbal dialogues that help build the therapist–patient working alliance and stronger bond through the depiction of empathy, respect and openness. ECAs (Embodied conversational agents) are human-like virtual agents that exhibit verbal and non-verbal behaviours. In the digital health space, ECAs act as health coaches or experts. ECA dialogues have previously been designed to include relational cues to motivate patients to change their current behaviours and encourage adherence to a treatment plan. However, there is little understanding of who finds specific relational cues delivered by an ECA helpful or not. Drawing the literature together, we have categorised relational cues into empowering, working alliance, affirmative and social dialogue. In this study, we have embedded the dialogue of Alex, an ECA, to encourage healthy behaviours with all the relational cues (empathic Alex) or with none of the relational cues (neutral Alex). A total of 206 participants were randomly assigned to interact with either empathic or neutral Alex and were also asked to rate the helpfulness of selected relational cues. We explore if the perceived helpfulness of the relational cues is a good predictor of users’ intention to change the recommended health behaviours and/or development of a working alliance. Our models also investigate the impact of individual factors, including gender, age, culture and personality traits of the users. The idea is to establish whether a certain group of individuals having similarities in terms of individual factors found a particular cue or group of cues helpful. This will establish future versions of Alex and allow Alex to tailor its dialogue to specific groups, as well as help in building ECAs with multiple personalities and roles.
Influences of Cu Doping on the Microstructure, Optical and Resistance Switching Properties of Zinc OxideThin Films
Authors: Weng Jun-Hong , Kao Ming-Cheng , Chen Kai-Huang , Li Men-Zhe
Published: October 2023

Copper-doped zinc oxide films (Zn1−xCuxO) (x = 0, 2%, 4%, 6%) were fabricated on conductive substrates using the sol-gel process. The crystal structure, optical and resistive switching properties of Zn1−xCuxO films are studied and discussed. RRAM is made using Zn1−xCuxO as the resistive layer. The results show that the (002) peak intensity and grain size of Zn1−xCuxOfilms increase from 0 to 6%. In addition, PL spectroscopy shows that the oxygen vacancy defect density of Zn1−xCuxO films also increases with the increase in Cu. The improved resistive switching performance of the RRAM device can be attributed to the formation of conductive filaments and the destruction of more oxygen vacancies in the Zn1−xCuxO film. Consequently, the RRAM device exhibits a higher low resistance state to high resistance state ratio and an HRS state of higher resistance value.
Radiolabeling of Micro-/Nanoplastics via In-Diffusion
Authors: Stricker Alexandra , Hilpmann Stephan , Mansel Alexander , Franke Karsten , Schymura Stefan
Published: October 2023

Micro- and nanoplastics are emerging pollutants with a concerning persistence in the environment. Research into their environmental impact requires addressing challenges related to sensitively and selectively detecting them in complex ecological media. One solution with great potential for alleviating these issues is using radiolabeling strategies. Here, we report the successful introduction of a 64Cu radiotracer into common microplastics, namely polyethylene, polyethylene terephthalate, polystyrene, polyamide, and polyvinylidene dichloride, which allows the sensitive detection of mere nanograms of substance. Utilizing a Hansen Solubility Parameter screening, we developed a swelling and in-diffusion process for tetraphenylporphyrin-complexed 64Cu, which permits one-pot labeling of polymer particles.
Unique Properties of Nutrient Channels on Plasmodium-Infected Erythrocytes
Authors: Desai Sanjay Arvind
Published: October 2023

Intracellular malaria parasites activate an ion and organic solute channel on their host erythrocyte membrane to acquire a broad range of essential nutrients. This plasmodial surface anion channel (PSAC) facilitates the uptake of sugars, amino acids, purines, some vitamins, and organic cations, but remarkably, it must exclude the small Na+ ion to preserve infected erythrocyte osmotic stability in plasma. Although molecular, biochemical, and structural studies have provided fundamental mechanistic insights about PSAC and advanced potent inhibitors as exciting antimalarial leads, important questions remain about how nutrients and ions are transported. Here, I review PSAC’s unusual selectivity and conductance properties, which should guide future research into this important microbial ion channel.
Molecular Characterization of Lyssaviruses Originating from Domestic and Wild Cats Provides an Insight on the Diversity of Lyssaviruses and a Risk of Rabies Transmission to Other Susceptible Mammals and Humans in South Africa
Authors: Tsie Kefentse , Ngoepe Ernest , Phahladira Baby , Khumalo Nelisiwe , Sabeta Claude
Published: October 2023

Rabies is one of the most significant public and veterinary health problems, causing approximately 59,000 human deaths annually in the developing countries of Asia and Africa. The aetiologic agent, a viral species of the Lyssavirus genus, is highly neurotropic and has a wide host range, including terrestrial mammals and several Chiropteran species. The Lyssavirus mokola (MOKV) was first isolated in the late 1960s from organ pools of shrews (Crocidura flavescens manni) in the Mokola forest (Nigeria). To date, at least 30 MOKV isolations have been confirmed, all exclusively from Africa, with 73% from southern Africa. There is limited knowledge about the epidemiology of MOKV, and the reservoir host species is unknown. Here, we report on the molecular characterization of rabies viruses originating from both domestic and African wild cats. A partial region of the lyssavirus genome, encoding the nucleoprotein, was amplified and sequenced. Nucleotide sequence analysis demonstrated that 98% of cats were infected with both the canid and mongoose rabies virus variants, as well as a rare lyssavirus, Lyssavirus mokola, from a domestic cat from Eswatini. Furthermore, the nucleotide sequence divergence between the recently identified MOKV isolate and the historical Lyssavirus mokola isolates ranged from 6.8% to 8.3%. This study further highlights the association between the potential host species of Lyssavirus mokola and the domestic cat as an incidental host, and the important role cats may play in rabies transmission dynamics in the country. Therefore, continuous vaccination of domestic cats against rabies is crucial, even after the elimination of dog-mediated rabies, as spillover related to sylvatic rabies cycles is likely to occur.
Two-Step Performance Optimization of CsPbBr3 Perovskite Nanocrystals for Wide Color Gamut Displays
Authors: Cai Junhu , Chen Xiaogang , Zhang Wenyan , Yang Longwen , Lin Zexi , Zhao Wenxiao , Ye Yun , Xu Sheng , Guo Tailiang , Chen Enguo
Published: October 2023

Owing to their composition-tunable and narrow emissions and high photoluminescence quantum yield (PLQY), inorganic halide perovskite quantum dots (IPQDs) are a promising option for wide color gamut displays. However, their practical applications have been limited by their lattice structure instability and surface defect states. Herein, CsPbBr3:KBF4@SiO2 with improved stability and optical properties is successfully synthesized with a two-step optimization of fluorine (F) anion doping and SiO2 in situ coating. Compared with bromide (Br), higher electronegativity and a smaller radius of F lead to stronger binding energy with Pb2+. Also, F anions can occupy surface Br vacancies. Then, benefiting from the acidic environment provided by BF4− hydrolysis, tetraethyl orthosilicate (TEOS) can be more easily hydrolyzed on the CsPbBr3:KBF4 surface to generate SiO2 coating, thus further passivating lattice defects and improving environmental stability. Importantly, the PLQY of CsPbBr3:KBF4@SiO2 achieves 85%, and the stability has been greatly improved compared with pure CsPbBr3. Finally, CsPbBr3:KBF4@SiO2/PDMS, CsPbI3/PDMS, and CsPbCl3/PDMS composites with narrow emissions are applied to replace traditional phosphors as color converters for direct-view light-emitting diode (LED) displays or liquid crystal display (LCD) backlights. The color gamut reaches 118.22% under the NTSC standard. Concerning the display field, it suggests likely applications in the future.
Quantitative Analysis of Seed Surface Tubercles in Silene Species
Authors: Rodríguez-Lorenzo José Luis , Martín-Gómez José Javier , Juan Ana , Tocino Ángel , Cervantes Emilio
Published: October 2023

In the Caryophyllaceae, seed surfaces contain cell protrusions, of varying sizes and shapes, called tubercles. Tubercles have long been described in many species, but quantitative analyses with measurements of size and shape are lacking in the literature. Based on optical photography, the seeds of Silene were classified into four types: smooth, rugose, echinate and papillose. Seeds in each of these groups have characteristic geometrical properties: smooth seeds lack tubercles and have the highest values of circularity and solidity in their lateral views, while papillose seeds have the largest tubercles and lowest values of circularity and solidity both in lateral and dorsal views. Here, tubercle width, height and slope, maximum and mean curvature values and maximum to mean curvature ratio were obtained for representative seeds of a total of 31 species, 12 belonging to Silene subg. Behenantha and 19 to S. subg. Silene. The seeds of the rugose type had lower values of curvature. Additionally, lower values of curvature were found in species of S. subg. Silene in comparison with S. subg. Behenantha. The seeds of S. subg. Behenantha had higher values of tubercle height and slope and higher values of maximum and average curvature and maximum to mean curvature ratio.
Ratoon Rice System of Production: A Rapid Growth Pattern of Multiple Cropping in China: A Review
Authors: Wu Wenge , Li Zhong , Xi Min , Tu Debao , Xu Youzun , Zhou Yongjin , Zhang Zhixing
Published: October 2023

In this review, the significance of ratoon rice was introduced, and the research status and development trends of ratoon rice were also summarized. It is pointed out that mechanically harvested ratoon rice is the developing direction of future ratoon rice. On this basis, we analyzed the relationship between the yield of ratoon rice and many factors, such as variety characteristics, sowing date, water control, fertilizer, and many others. It is important to construct a comprehensive and practical evaluation system for rice regeneration that can provide a basis for high-yield cultivation of machine-harvested ratoon rice. At the same time, it is suggested that combining high-yield cultivation with the green ecological efficiency of rice can achieve better production and improve the quality of rice. Finally, some problems with ratoon rice development were put forward. An in-depth study on the rhizosphere biology and regulation techniques of ratoon rice and the effective ecological compensation mechanism increased the capacity and quality of ratoon rice. Further, the functioning of such research can enhance the planting area for ratoon rice and improve food security.
Aridity Gradients Shape Intraspecific Variability of Morphological Traits in Native Ceratonia siliqua L. of Morocco
Authors: Kassout Jalal , Hmimsa Younes , Fatehi Salama El , Kadaoui Khalil , Houssni Mhammad , Chakkour Soufian , Sahli Abdelouahab , El Chami Mohamad Ali , Ariza-Mateos David , Palacios-Rodríguez Guillermo , Navarro-Cerrillo Rafael M. , Ater Mohamed
Published: October 2023

The carob tree (Ceratonia siliqua L.) is a significant fruit tree in the Mediterranean region with cultural, biological, and ecological importance. Despite its importance, intraspecific trait variability (ITV) in carob trees has been largely overlooked in previous studies. Understanding ITV and its relationship with environmental conditions is crucial for conservation and breeding programs. In this study, we investigated the variability of carob pod and seed-related traits across different ecological scales in 25 studied populations in Morocco. Significant differences in morphological traits were observed between carob populations at various ecological levels, and pod-related traits exhibited greater variability than seed traits. Correlation analysis revealed strong associations between carob morphological traits and environmental conditions, with altitude and aridity index playing an influential role. The aridity gradient was strongly related to changes in pod size, seed number, and size, as well as seed yield. Our findings highlight an important ITV reaching 45% at the intra-population level, 36.5% at the inter-geographic level, and 30% at the inter-population level. Overall, this study contributes valuable insights into the ecology and adaptation of carob trees, emphasizing the importance of considering intraspecific variability when studying this remarkable species. This knowledge is critical for addressing the challenges posed by climate change and human activities on the long-term survival and ecological functioning of carob populations.
Resprouting Response among Savanna Tree Species in Relation to Stem Size, Woody Removal Intensity and Herbicide Application
Authors: Monegi Piet , Mkhize Ntuthuko Raphael , Tjelele Julius Tlou , Ward David , Tsvuura Zivanai
Published: October 2023

Mechanical and chemical methods are widely used to control woody plant encroachment in many African countries. However, very little is known about the effectiveness of these control methods among woody species of different ages. We conducted a field experiment to determine the effects of different tree removal treatments (10%, 20%, 50%, 75% and 100%) and herbicide application (Picloram; 6 mL L−1) on the resprouting ability and vigour of 12 woody plant species. We examined 20 plots (30 m × 30 m) that were each subjected to tree removal, followed by herbicide application on half of the stems for each plot. All the tree species in this study resprouted after cutting. The applied concentration of herbicide significantly reduced the shoot production for Ehretia rigida, Vachellia robusta and Ziziphus mucronata, with a marginal effect for Dichrostachys cinerea. The diameter of stems was an important factor in determining resprouting ability, with shoot production decreasing with increasing stem diameter. However, stem diameter did not affect shoot length and diameter for all species. We found that woody plants are more likely to resprout and survive as juveniles than as adults after cutting and that herbicide only affected four of the twelve species at a concentration of 6 mL L−1. Thus, testing the amount of Picloram needed to kill certain woody species may be of importance for land users in southern African savannas.
Vascular Plant Species Inventory of Mexico’s Revillagigedo National Park: Awareness of Alien Invaders as a Sine Qua Non Prerequisite for Island Conservation
Authors: Domínguez-Meneses Alejandra , Martínez-Gómez Juan Esteban , Mejía-Saulés Teresa , Acosta-Rosado Israel , Stadler Stefan
Published: October 2023

The Revillagigedo Archipelago, located in the Eastern Pacific Ocean, stands out for its unique biological richness and endemism. These islands remained uninhabited until the second half of the twentieth century, allowing a better conservation status than on other oceanic islands. However, the continuous introduction of potentially invasive alien plant species, and the lack of adequate control or eradication actions, jeopardize the conservation and restoration of these islands’ fragile ecosystems. We present the most complete vascular plant species inventory and an updated list of alien plant species of the Revillagigedo Archipelago, which was compiled through an extensive review of national and international plant collections and other sources. Our 272 species list includes 106 alien plant species (39.3%; 104 in Socorro, and 16 in Clarion): 67 (24.8%) are naturalized, 14 (5.2%) are casual aliens, and 25 (9.3%) subsist under cultivation. The documented alien species belong to 73 families. Annual and perennial herbs are the prevailing life forms in the alien flora, while naturalized species are primarily native to North America. The number of introduced species has increased significantly since the islands became inhabited. Many of the recently introduced species pose a major invasion risk like on other islands of the world.
Phytochemical and Agronomic Characterization of High-Flavonoid Lettuce Lines Grown under Field Conditions
Authors: Cho Eunjin , Gurdon Csanad , Zhao Rebecca , Peng Hui , Poulev Alexander , Raskin Ilya , Simko Ivan
Published: October 2023

Flavonoids are antioxidant phytochemicals that confer a beneficial effect on human health. We have previously developed and characterized eight lettuce (Latuca sativa L.) lines that accumulated high levels of diverse flavonoids and their precursors in controlled environment conditions. Three Rutgers Scarlet lettuce (RSL) lines selected in tissue culture for deep-red color (RSL-NAR, RSL-NBR, RSL-NFR) accumulate anthocyanins and quercetin, three lines identified in a chemically mutagenized red lettuce population accumulate kaempferol (KfoA and KfoB) or naringenin chalcone (Nco), and two lines that were spontaneous green mutants derived from the red line RSL-NAR (GSL, GSL-DG) accumulate quercetin. These eight lines were field-grown in the Salinas Valley of California for four years together with seven control accessions of varying colors (light green, dark green, red, and dark red). At market maturity, a substantial variation in plant composition was observed, but the three RSL lines consistently accumulated high levels of cyanidin, GSL and GSL-DG accumulated the highest levels of quercetin, KfoA and KfoB accumulated kaempferol, and Nco amassed naringenin chalcone, confirming that these mutant lines produce high levels of beneficial phytochemicals under field conditions. Mutant lines and control accessions were also assessed for their biomass production (plant weight, height, and width), overall content of pigments (leaf chlorophyll and anthocyanins), resistance to diseases (downy mildew, lettuce drop, and Impatiens necrotic spot virus), postharvest quality of processed tissue (deterioration and enzymatic discoloration), and composition of 23 mineral elements. All but one mutant line had a fresh plant weight at harvest comparable to commercial leaf cultivars; only Nco plants were significantly (p < 0.05) smaller. Therefore, except for Nco, the new, flavonoid hyperaccumulating lines can be considered for field cultivation.
Suitability of Mycelium-Reinforced Nanofiber Mats for Filtration of Different Dyes
Authors: Heide Angela , Wiebe Philip , Sabantina Lilia , Ehrmann Andrea
Published: October 2023

Electrospun nanofiber mats have a high specific surface area and very small pores which can be tailored by the spinning process. They are thus highly suitable as filters for small particles and molecules, such as organic dyes. On the other hand, they are usually very thin and thus have low mechanical properties. As a potential reinforcement, mycelium of Pleurotus ostreatus was grown on poly(acrylonitrile) nanofiber mats and thermally solidified after fully covering the nanofiber mats. This study investigates whether the filtration efficiency of the nanofiber mats is altered by the mycelium growing through it and whether the mechanical properties of the nanofibrous filters can be improved in this way. The study shows fast and reliable growth of the mycelium on the nanofiber mats and high filtration efficiency for astra blue and chlorophyll, while indigo carmine showed only very low filtration efficiency of up to 20%. For chlorophyll and safranin, membranes with mycelium showed higher filtration than pure nanofiber mats. In diffusion cell tests, especially astra blue was strongly adsorbed on the membranes with mycelium.
Persistence Length of PEGMA Bottle Brushes Determined by Pyrene Excimer Fluorescence
Authors: Thoma Janine L. , Little Hunter , Duhamel Jean , Zhang Lei , Leung Kam Tong
Published: October 2023

Seven pyrene-labeled poly(oligo(ethylene glycol) methyl ether methacrylate)s (PyEG5-PEGnMAs) were prepared with n = 0, 3, 4, 5, 7, 9, and 19 ethylene glycol units by copolymerizing a small amount of penta(ethylene glycol) 1-pyrenemethyl ether methacrylate with an EGnMA monomer. The conformation of the PyEG5-PEGnMA polymers evolved from a random coil for PyEG5-PEG0MA or poly(methyl methacrylate) to a polymeric bottle brush (PBB) architecture with increasing side chain length. The fluorescence decays of the PyEG5-PEGnMA samples were fitted according to the fluorescence blob model (FBM) whose parameters were used, in combination with the Kratky–Porod equation, to calculate the persistence length of these polymers. The persistence lengths obtained from the PEF experiments were found to increase with the square of the number (NS) of non-hydrogen atoms in the side chain as expected theoretically. The persistence lengths found with the PyEG5-PEGnMA samples in DMF also matched those found earlier for another series of PEGnMA samples labeled with 1-pyrenebutanol. The good agreement found between the persistence lengths obtained with the PEGnMA samples labeled with two different pyrene derivatives illustrates the robustness of the method and its applicability for measuring the unknown persistence length of polydisperse polymer samples.
Influence of Bentonite Particles on the Mechanical Properties of Polyester–Sisal Fiber Composites
Authors: Valin Rivera José Luis , Valenzuela Reyes Cristian Rodolfo , Quinteros Wachtendorff Arturo Andrés , Rodríguez Soto Angel , Valin Fernández Meylí , Iquilio Abarzúa Roberto , González Ortega Alvaro , García del Pino Gilberto , Valenzuela Diaz Francisco Rolando
Published: October 2023

As a part of the mission to create materials that are more environmentally friendly, we present the following proposal, in which a study of the mechanical properties of composite materials comprising a polyester resin with sisal fiber and bentonite particles was conducted. Sisal fiber was added to a matrix in percentages ranging from 5% to 45% in relation to the polyester resin weight, while bentonite remained fixed at 7% in relation to the polyester resin weight. The specimens were manufactured by compression molding. The mechanical properties were analyzed by tensile, bending, impact, stepped creep, and relaxation tests. In addition, energy-dispersive X-ray spectroscopy and scanning electron microscopy analyses were carried out to analyze the composition and heterogeneity of the structure of the composite material. The results obtained showed that 7% of bentonite added to the matrix affects the tensile strength. Flexural strength increased by up to 21% in the specimens with a 20% addition of sisal fiber, while the elastic modulus increased by up to 43% in the case of a 20% addition of sisal fiber. The viscoelastic behavior was improved, while the relaxation stress was affected.
A Brief Overview on Epoxies in Electronics: Properties, Applications, and Modifications
Authors: Dallaev Rashid , Pisarenko Tatiana , Papež Nikola , Sadovský Petr , Holcman Vladimír
Published: October 2023

This paper offers a short overview of epoxy resins, encompassing their diverse characteristics, variants, chemical modifications, curing processes, and intriguing electrical properties. Epoxies, valued for their multifunctional attributes, serve as fundamental materials across industries. In the realm of dielectric strength, epoxy resins play a crucial role in electrical insulation. This paper discusses the mechanisms governing dielectric breakdown, strategies to enhance dielectric strength, and the impact of various fillers and additives on insulation performance. Through an exploration of recent research and advancements, this paper delves into the spectrum of epoxy properties, the array of subspecies and variants, their chemical adaptability, and the intricacies of curing. The examination of electrical resistance and conductivity, with a focus on their frequency-dependent behavior, forms a pivotal aspect of the discussion. By shedding light on these dimensions, this review provides a concise yet holistic understanding of epoxies and their role in shaping modern materials science.
Biodegradable Nanofiber/Metal–Organic Framework/Cotton Air Filtration Membranes Enabling Simultaneous Removal of Toxic Gases and Particulate Matter
Authors: Ryu Sujin , Kim Doyeon , Lee Hyewon , Kim Yoonjin , Lee Youngbok , Kim Myungwoong , Lee Heedong , Lee Hoik
Published: October 2023

The typical filters that protect us from harmful components, such as toxic gases and particulate matter (PM), are made from petroleum-based materials, which need to be replaced with other environmentally friendly materials. Herein, we demonstrate a route to fabricate biodegradable and dual-functional filtration membranes that effectively remove PM and toxic gases. The membrane was integrated using two layers: (i) cellulose-based nanofibers for PM filtration and (ii) metal–organic framework (MOF)-coated cotton fabric for removal of toxic gases. Zeolitic imidazolate framework (ZIF-8) was grown from the surface of the cotton fabric by the treatment of cotton fabric with an organic precursor solution and subsequent immersion in an inorganic precursor solution. Cellulose acetate nanofibers (NFs) were deposited on the MOF-coated cotton fabric via electrospinning. At the optimal thickness of the NF layer, the quality factor of 18.8 × 10−2 Pa−1 was achieved with a filtration efficiency of 93.1%, air permeability of 19.0 cm3/cm2/s, and pressure drop of 14.2 Pa. The membrane exhibits outstanding gas adsorption efficiencies (>99%) for H2S, formaldehyde, and NH3. The resulting membrane was highly biodegradable, with a weight loss of 62.5% after 45 days under standard test conditions. The proposed strategy should provide highly sustainable material platforms for practical multifunctional membranes in personal protective equipment.
MC-Injection Molding with Liquid Silicone Rubber (LSR) and Acrylonitrile Butadiene Styrene (ABS) for Medical Technology
Authors: Nikousaleh Mohammad Ali , Giesen Ralf-Urs , Heim Hans-Peter , Hartung Michael
Published: October 2023

The multicomponent injection molding of liquid silicone rubbers (LSR) with thermoplastics, such as polybutylene terephthalate (PBT) or polyamide (PA), is a state-of-the-art technique and is used in the manufacturing process for many components in the automotive industry and in the field of sanitary engineering. Standard thermoplastics, such as acrylonitrile butadiene styrene (ABS), cannot be bonded with silicone rubbers in injection molding because of their low heat deflection temperature. In this study, we investigated ABS grades approved for medical applications to show how dynamic mold heating and various pretreatment methods for thermoplastic surfaces can be used to produce ABS-LSR test specimens. In addition, such components’ sterilization effect on the adhesive bond will be shown.
Controlling Eutrophication of Aquaculture Production Water Using Biochar: Correlation of Molecular Composition with Adsorption Characteristics as Revealed by FT-ICR Mass Spectrometry
Authors: Bare William F. Rance , Struhs Ethan , Mirkouei Amin , Overturf Kenneth , Chacón-Patiño Martha L. , McKenna Amy M. , Chen Huan , Raja Krishnan S.
Published: October 2023

This study aims to construct a novel and sustainable approach for remediating aquaculture-generated water contamination using various engineered biochars. Particularly, this study focuses on capturing nitrogen and phosphorus from downstream water of commercial fish farms in Magic Valley, Idaho, containing approximately 2.26 mg/L of nitrogen and 0.15 mg/L of phosphorous. The results indicate that the proposed approach can improve downstream waters by adsorbing micronutrients (e.g., nitrogen-ammonia, nitrate-n + nitrite-n, and total phosphorus). Water treatment time and biochar pH are two key parameters strongly associated with adsorbed compounds. Molecular-level characterization of solvent-extracted organics from biochar materials (before and after water treatment) suggests increased levels of highly oxygenated molecules as a function of increasing water treatment time. Also, the results show the enrichment in organic species with higher molecular weight and increased double bond equivalents, with a compositional range similar to that of dissolved organic matter. Upon water treatment, extracted organics revealed higher abundances of compounds with higher H/C and O/C ratios. The engineered biochars, after water treatment, can be reused as nutrient-rich fertilizers. This study concluded that the engineered biochars could sequester more nitrogen and phosphorous over time. Also, the proposed approach can simultaneously increase fish production capacity and support the aquaculture industry in different regions by improving water quality and enabling aquaculture expansion.
Preparation and Characterization of Corn Straw-Based Graphitized Carbon with Ferric Acetylacetonate as Catalyst
Authors: Mu Li , He Peng , Liu Pengfei
Published: October 2023

Graphitized carbon exhibits exceptional thermal stability, electrical conductivity, corrosion resistance, and various intricate physical and chemical properties. Consequently, it has found extensive applications in diverse fields, such as electrodes, refractory materials, nuclear reactors, and supercapacitors. However, natural graphite is a limited nonrenewable resource, so finding other materials, exploring reliable graphitization methods, and achieving efficient green graphite production as an essential trend in the future is essential. In this paper, with corn straw liquefied product (CSLP) as raw material, ferric acetone catalyst, using carbonization, catalytic graphitization preparation of corn straw based graphitic carbon (CSBGC). When the graphitization temperature was 850 °C and the amount of ferric acetylpropionate (Fe(acac)3) was 7.0 mmol/g, the graphitized carbon showed better graphitization, micro fragmentation structure, and more minor defects, which effectively reduced the graphitization temperature, and the graphitic carbon rate of corn straw (CS) reached 25.2%. This study not only presents a highly efficient approach for synthesizing superior biomass-derived graphite carbon but also introduces usable perspectives on using corn straws.
A Time-Saving Approach to Parameter Studies in Microwave-Assisted Freeze Drying
Authors: Sickert Till , Bergmann Richy , Christoph Jana , Gaukel Volker
Published: October 2023

Microwave-assisted freeze drying (MFD) is particularly suited for drying heat-sensitive materials. However, optimizing process parameters is time-consuming due to lengthy individual experiments. This study investigates the feasibility of partial MFD for parameter studies, with the process being terminated after removing 20 wt% of the water contained. The proposed approach reduces the time required for parameter evaluation by 92.0% compared to complete MFD. It also enables the evaluation of the samples’ drying homogeneity. A subsequent parameter study based on partial MFD was then conducted to evaluate the effects of chamber pressure, microwave power, and microwave frequencies on the drying kinetics and drying homogeneity. Lowering the average chamber pressure from 0.87 mbar to 0.19 mbar reduced the process duration by 18.8%. An increase in the dissipated specific microwave power from 0.048 W/g to 0.143 W/g reduced the duration by 46.7%. The targeted application of frequencies increased the average energy efficiency to as high as 92.4%, contributing to a shortened process duration of up to 51.2%. Only the application of multiple frequencies caused a notable increase in drying homogeneity. In summary, this study demonstrates the feasibility and time-saving benefits of partial drying for parameter studies in MFD and potentially different types of drying processes.
Investigating Erosion of String in Underground Hydrogen Storage under High Flow Velocity
Authors: Zhu Lixia , Li Lifeng , Luo Jinheng , Han Ziyue , Xie Shuyi , Yu Tao , Liu Qing
Published: October 2023

Underground hydrogen storage represents an innovative approach to energy storage. To ensure the secure operation of subterranean hydrogen storage strings, a computational fluid dynamics (CFD) methodology was employed to devise an erosion assessment model tailored for high-velocity conditions. The research delved into the erosion and abrasion dynamics of these storage strings when subjected to high-speed gas flows. This study further examined the impacts of gas velocity, particle size, pipe material, and pipe wall corrosion imperfections on flow patterns and erosion wear rates across the column. The outcomes revealed several noteworthy trends. As fluid velocity increased, the flow field’s maximum pressure augmented, while it decreased alongside enlarging pipe diameter and particle size. P110 pipe material exhibited higher maximum pressure in comparison to N80. The effect of centrifugal force induced pressure to surge from the inner to the outer portion of the column. In the curved pipe section’s outer wall, the frequent occurrence of high-angle collisions engendered elevated rates of erosion wear over time. Particularly noteworthy was the observation of the highest erosion rate in curved pipes showcasing three corrosion defects, attributed to the backflow effects of erosion pits.
Python in Chemistry: Physicochemical Tools
Authors: Ryzhkov Fedor V. , Ryzhkova Yuliya E. , Elinson Michail N.
Published: October 2023

The popularity of the Python programming language in chemistry is growing every year. Python provides versatility, simplicity, and a rich ecosystem of libraries, making it the preferred choice for solving chemical problems. It is widely used for kinetic and thermodynamic calculations, as well as in quantum chemistry and molecular mechanics. Python is used extensively for laboratory automation and software development. Data analysis and visualization in chemistry have also become easier with the libraries available in Python. The evolution of theoretical and computational chemistry is expected in the future, especially at intersections with other fields such as machine learning. This review presents tools developed for applications in kinetic, thermodynamic, and quantum chemistry, instruments for molecular mechanics, and laboratory equipment. Online courses that help scientists without programming experience adapt Python to their chemical problems are also listed.
Performance Evaluation and MOORA Based Optimization of Pulse Width Control on Leather Specimens in Diode Laser Beam Cutting Process
Authors: Khalaf Tamer , Thangaraj Muthuramalingam , Moiduddin Khaja
Published: October 2023

Due to the variety of benefits over traditional cutting techniques, the usage of laser cutting technology has risen substantially in recent years. The attributes of laser technology for leather cutting include adaptability, mass production, capability of cutting complicated patterns, ease of producing tailored components, and reduction in leather waste. In the present study, vegetable chrome-tanned buffalo leather specimens were cut using a 20 W laser diode with conventional and pulse width control in a photodiode-assisted laser cutting process. Emission rate, kerf width, carbonization, and material removal rate were considered as quality indicators. The higher power density associated with the pulse width approach reduces the interaction with the specimen, which results in a better emission rate and material removal rate, along with a lesser kerf width and carbonization. Using the MOORA approach, the optimal parameters of the present study were found to be a stand-off distance of 22 mm, a feed rate of 200 mm/min, a duty cycle of 75%, and a frequency of 20 kHz. The duty cycle can effectively control the pulse width at which the energy has been dissipated across the cutting zone.
Hydraulic Characterization of Variable-Speed Pump Turbine under Typical Pumping Modes
Authors: Shang Linmin , Cao Jingwei , Wang Zhengwei , Liu Xiaobing
Published: October 2023

The pump turbine is a crucial component of pumped storage hydropower plants. When operated at a constant speed, it does not respond well to variations in the grid frequency. To improve the hydraulic efficiency of pumped storage units, variable-speed units have been introduced. However, the mechanism of variable-speed pump turbines has not been extensively studied numerically. In this study, the flow characteristics of a variable-speed pump turbine were computed under two typical pumping modes, the maximum head and minimum flow rate condition, as well as the minimum head and maximum flow rate condition. The computed results aligned with experimental results, and the changing trends of hydraulic thrust under these two pumping modes were discussed. The error for the Hmax, Qmin condition was 1.3%, and the error for the Hmin, Qmax condition was −1.9%. These error values fell within a reasonable range. The research findings indicate that in the Hmax, Qmin condition, the flow within the flow passage exhibited higher velocity, which was 84.87 m/s, increased flow turbulence, larger pressure fluctuations, and poorer unit stability. On the other hand, in the Hmin, Qmax condition, both the axial hydraulic thrust and radial forces were greater, and there were sudden changes in the extreme values of pressure fluctuations over a certain period of time. It is recommended to avoid operating the variable-speed pump turbine under these two conditions during pumping operations.
Studies on a Novel Jet Mixer in the Extraction Process
Authors: Piotrowski Wojciech , Kubica Robert , Gądek Maksymilian
Published: October 2023

This paper presents the original results of research on an inline jet mixer being an alternative to other, conventional mixing apparatuses used for extraction processes. In particular, researched novel geometry of a jet mixer was subjected to testing of either hydraulic performance or a liquid–liquid extraction process. Inline jet mixers are well suited for mixing gases and liquids and can be used in such processes as extraction, heat exchange, and reaction. In such an apparatus, mixing of liquids takes place by high-velocity injection of one stream into another through a series of small holes placed peripherally to a concentrically mounted inner tube. The literature lacks the data to allow for the design of these types of mixers. Extraction experiments were performed for the ethyl acetate–ethanol–water system. The research results presented in this paper enable the calculation of mixing power and the selection of optimal mixer operating parameters. Equations describing the flow resistance for both streams were developed. The mixing power was calculated and compared with other types of contactors. The data on overall volumetric mass transfer coefficients obtained by this study showed that the considered extractor is competitive with other conventional contactors at almost identical or even lower energy consumption.