bluuebunny/crossref_metadata_2025 huggingface dataset:
Effects of Transcutaneous Electrical Nervous Stimulation (TENS) on Dysphonic Patients: A Systematic Review Study
Authors: Plotas Panagiotis , Papadopoulos Angelos , Tsiamaki Eirini , Apostolou Maria-Dimitra , Chaniotaki Maria-Antonia , Ganiatsou Efthimia , Goutzeri Eleni-Marianthi , Kalogeraki Thalia , Karra Elpida , Malliou Maria , Marinitsi Dimitra , Papoutsaki Chariklia , Vagianou Ilianna-Stamatia , Trimmis Nikolaos
Published: September 2023

Background and Objectives: Transcutaneous electrical nerve stimulation (TENS), a pain-alleviating and muscle-relaxing treatment used in physio-therapeutic clinical practice, has recently appeared to be just as effective in dysphonia. This review aimed at clarifying whether TENS can be an effective practice in dysphonia therapy and/or management on its own or combined with other types of interventions and, hence, whether its practice can be a useful, more widespread establishment to speech and language therapy intervention methods. Materials and Methods: A search was conducted on the PubMed database using specific terms based on the PICO search strategy. Eventually, four randomized controlled studies and four clinical trials were included. The methodological quality of the included studies was evaluated using the physiotherapy evidence-based database (PEDro) assessment tool, and this indicated high-quality research with an average score of 8.43. Results: The studies utilized various TENS devices, predominantly the Dualpex 961 device (frequency of 10 Hz, phase of 200 ms). The assessment methods varied, including auditory perception, vocal therapy, electrostimulation, audio and video perceptual assessments, and laryngeal evaluations. The clinical outcomes of TENS showed a reduction in musculoskeletal pain in various areas, while the acoustic analysis results were significant in only one study. TENS was compared to manual laryngeal therapy (LMT), placebo TENS, and vocal therapy in different studies with mixed results. Conclusions: This review supports the idea that a multidimensional approach, incorporating various therapeutic modalities (TENS, LMT, speech therapy, and vocal training) can yield positive outcomes for patients with voice disorders. Further research is needed to explore the specific mechanisms of action and optimal treatment protocols for TENS in voice therapy.
Application of Ultrafiltration for Recovery of Polyphenols from Rose Petal Byproduct
Authors: Dushkova Mariya , Vardakas Alexios , Shikov Vasil , Mihalev Kiril , Terzyiska Margarita
Published: September 2023

One main objective of this study was to increase the utilization of raw material in the rose (Rosa damascena Mill.) essential oil industry by the application of membrane technologies. In this research, distilled (dearomatized) rose petals, the primary byproduct in essential oil production, were subjected to an enzyme-assisted extraction and subsequent membrane separation for partial concentration at different levels using UF1-PAN and UF10-PAN membranes. The results show that the permeate flux decreased with a rise in volume reduction ratio and increased with a rise in transmembrane pressure and feed flow rate. At the beginning of the process, the highest flux was with the UF1-PAN membrane, but at the end of the process, it was with the UF10-PAN membrane. Total polyphenols of the retentates increased by 27–39% and 26–67% during ultrafiltration with the UF1-PAN and UF10-PAN membranes, respectively, with the highest value obtained for the UF10-PAN membrane at VRR 6. The highest concentration factor and rejection of total solids, total polyphenols, redox-active antioxidants, and radical scavenging antioxidants were obtained at VRR 6 with the UF10-PAN membrane. The use of green technology based on enzyme-assisted extraction and ultrafiltration for recovery and concentration of polyphenols from rose petal byproduct solves practical environmental problems for the treatment and utilization of byproducts from the rose oil industry. The retentate obtained could be used in the food production, cosmetic, and pharmaceutical industries.
Towards Understanding the Relationships between Processing Conditions and Mechanical Performance of the Additive Friction Stir Deposition Process
Authors: Williams Malcolm B. , Zhu Ning , Palya Nick I. , Hoarston Jacob B. , McDonnell Martin M. , Kelly Matthew R. , Lalonde Aaron D. , Brewer Luke N. , Jordon James B. , Allison Paul G.
Published: September 2023

In this research, we explore the preliminary effects of processing conditions using a novel additive manufacturing (AM) process, known as additive friction stir deposition (AFSD), on resulting build direction (BD) mechanical performance. Using the AFSD process, a feasibility study of three AM builds of identical size are created using differentiating processing parameters. A relationship referred to as the deposition pitch, exhibiting similarities to weld pitch, is determined to be a simple but effective predictor of the interlayer bonding in AFSD processing of AA7020. The deposition pitch directly correlates the necessary temperature, time, and pressure required for effective solid-state bonding. Using this correlation, increased mechanical performance in the BD is achieved through an increase in deposition pitch. A reduction in the deposition pitch from 4.46 rev/mm to 1.08 rev/mm resulted in a significant decrease in failure strain from 24.4% to 0.82%, with the failure mechanism shifting from a ductile failure to brittle failure. The inverse relationship between grain refinement and BD failure strain at high deposition pitches suggests deposition pitch and heat input are the dominant factors in the resulting BD mechanical properties.
Exploring the Potential of Methanotrophs for Plant Growth Promotion in Rice Agriculture
Authors: Mohite Jyoti A. , Khatri Kumal , Pardhi Kajal , Manvi Shubha S. , Jadhav Rutuja , Rathod Shilpa , Rahalkar Monali C.
Published: September 2023

Rice fields are one of the important anthropogenic sources of methane emissions. Methanotrophs dwelling near the rice roots and at the oxic–anoxic interface of paddy fields can oxidize a large fraction of the generated methane and are therefore considered to be important. Nitrogen fixation in rice root-associated methanotrophs is well known. Our aim in this study was to explore the potential of methanotrophs as bio-inoculants for rice and the studies were performed in pot experiments in monsoon. Ten indigenously isolated methanotrophs were used belonging to eight diverse genera of Type Ia, Type Ib, and Type II methanotrophs, including the newly described genera and/or species, Methylocucumis oryzae and Methylolobus aquaticus, as well as Ca. Methylobacter oryzae and Ca. Methylobacter coli. Additionally, two consortia (Methylomonas strains and Methylocystis-Methylosinus strains) were used. Nitrogen fixation pathways or nifH genes were detected in all of the used methanotrophs. Plant growth promotion (PGPR) was seen in terms of increased plant height and grain yield. Nine out of twelve (seven single strains and two consortia) showed positive effects on grain yield (6–38%). The highest increase in grain yield was seen after inoculation with Ca. Methylobacter coli (38%) followed by Methylomonas consortium (35%) and Methylocucumis oryzae (31%). Methylomagnum ishizawai inoculated plants showed the highest plant height. Methylocucumis oryzae inoculated plants showed early flowering, grain formation, and grain maturation (~17–18 days earlier). In all the pot experiments, minimal quantities of nitrogen fertilizer were used with no additional organic fertilizer inputs. The present study demonstrated the possibility of developing methanotrophs as bio-inoculants for rice agriculture, which would promote plant growth under low inputs of nitrogenous fertilizers. Although the effect of methanotrophs on methane mitigation is still under investigation, their application to reduce methane emissions from rice fields could be an added advantage.
Integration of Inkjet Printed Graphene as a Hole Transport Layer in Organic Solar Cells
Authors: Kastner Julia , Tomarchio Flavia , Decorde Nicolas , Kehrer Matthias , Hesser Günter , Fuchsbauer Anita
Published: September 2023

This work demonstrates the green production of a graphene ink for inkjet printing and its use as a hole transport layer (HTL) in an organic solar cell. Graphene as an HTL improves the selective hole extraction at the anode and prevents charge recombination at the electronic interface and metal diffusion into the photoactive layer. Graphite was exfoliated in water, concentrated by iterative centrifugation, and characterized by Raman. The concentrated graphene ink was incorporated into inverted organic solar cells by inkjet printing on the active polymer in an ambient atmosphere. Argon plasma was used to enhance wetting of the polymer with the graphene ink during printing. The argon plasma treatment of the active polymer P3HT:PCBM was investigated by XPS, AFM and contact angle measurements. Efficiency and lifetime studies undertaken show that the device with graphene as HTL is fully functional and has good potential for an inkjet printable and flexible alternative to PEDOT:PSS.
Positive and Negative Photoconductivity in Ir Nanofilm-Coated MoO3 Bias-Switching Photodetector
Authors: Basyooni-M. Kabatas Mohamed A. , En-nadir Redouane , Rahmani Khalid , Eker Yasin Ramazan
Published: September 2023

In this study, we delved into the influence of Ir nanofilm coating thickness on the optical and optoelectronic behavior of ultrathin MoO3 wafer-scale devices. Notably, the 4 nm Ir coating showed a negative Hall voltage and high carrier concentration of 1.524 × 1019 cm−3 with 0.19 nm roughness. Using the Kubelka–Munk model, we found that the bandgap decreased with increasing Ir thickness, consistent with Urbach tail energy suggesting a lower level of disorder. Regarding transient photocurrent behavior, all samples exhibited high stability under both dark and UV conditions. We also observed a positive photoconductivity at bias voltages of >0.5 V, while at 0 V bias voltage, the samples displayed a negative photoconductivity behavior. This unique aspect allowed us to explore self-powered negative photodetectors, showcasing fast response and recovery times of 0.36/0.42 s at 0 V. The intriguing negative photoresponse that we observed is linked to hole self-trapping/charge exciton and Joule heating effects.
Evaluation of Biocontrol Potential of Bacillus spp. and Pseudomonas fluorescens UM270 against Postharvest Fungal Pathogens
Authors: Morales-Cedeño Luzmaria R. , Barajas-Barrera Ignacio A. , Parra-Cota Fannie I. , Valenzuela-Ruiz Valeria , de los Santos-Villalobos Sergio , Loeza-Lara Pedro D. , Herrera-Pérez Alejandra , del Carmen Orozco-Mosqueda Ma. , Santoyo Gustavo
Published: September 2023

Fungal pathogens are the main causal agents of postharvest diseases of fruits and vegetables. To prevent this problem and avoid the use of harmful chemical fungicides, safer and greener alternatives have been sought. One of these alternatives is the use of plant-growth-promoting bacteria (PGPB). In this study, we evaluated in vitro four well-known PGPB strains (Pseudomonas fluorescens UM270, Bacillus toyonensis COPE52, Bacillus sp. E25, and Bacillus thuringiensis CR71) for their biocontrol potential against nineteen postharvest fungal pathogens. In vivo assays were also performed, and bacterial cells were inoculated on harvested strawberries and grapes with the pathogens Botrytis cinerea, Alternaria alternata, and Fusarium brachygibbosum to evaluate loss of firmness and disease incidence. Our results show that the four strains antagonized fungi in direct and indirect confrontation assays. Stronger antagonism was observed by the action of diffusible metabolites (DMs) compared to volatile organic compound (VOC) activity. All PGPB significantly improved the fruit firmness and reduced disease incidence caused by the fungal pathogens tested. However, strain UM270 showed excellent biocontrol activity, reducing the disease incidence of Fusarium brachygibbosum, Botrytis cinerea, and Alternaria alternata on strawberry fruits by 60%, 55%, and 65%, respectively. Diffusible antifungals and VOCs such as 2,4-diacetyl phloroglucinol, siderophores, auxins, fengycins, and N, N-dimethyl-hexadecyl amine, among others, might be responsible for the beneficial activities observed. These results suggest excellent biocontrol activities to inhibit postharvest pathogenic fungi and improve harvested fruit quality.
Comparative De Novo and Pan-Genome Analysis of MDR Nosocomial Bacteria Isolated from Hospitals in Jeddah, Saudi Arabia
Authors: Alghamdi Molook , Al-Judaibi Effat , Al-Rashede Mohammed , Al-Judaibi Awatif
Published: September 2023

Multidrug-resistant (MDR) bacteria are one of the most serious threats to public health, and one of the most important types of MDR bacteria are those that are acquired in a hospital, known as nosocomial. This study aimed to isolate and identify MDR bacteria from selected hospitals in Jeddah and analyze their antibiotic-resistant genes. Bacteria were collected from different sources and wards of hospitals in Jeddah City. Phoenix BD was used to identify the strains and perform susceptibility testing. Identification of selected isolates showing MDR to more than three classes on antibiotics was based on 16S rRNA gene and whole genome sequencing. Genes conferring resistance were characterized using de novo and pan-genome analyses. In total, we isolated 108 bacterial strains, of which 75 (69.44%) were found to be MDR. Taxonomic identification revealed that 24 (32%) isolates were identified as Escherichia coli, 19 (25.3%) corresponded to Klebsiella pneumoniae, and 17 (22.67%) were methicillin-resistant Staphylococcus aureus (MRSA). Among the Gram-negative bacteria, K. pneumoniae isolates showed the highest resistance levels to most antibiotics. Of the Gram-positive bacteria, S. aureus (MRSA) strains were noticed to exhibit the uppermost degree of resistance to the tested antibiotics, which is higher than that observed for K. pneumoniae isolates. Taken together, our results illustrated that MDR Gram-negative bacteria are the most common cause of nosocomial infections, while MDR Gram-positive bacteria are characterized by a wider antibiotic resistance spectrum. Whole genome sequencing found the appearance of antibiotic resistance genes, including SHV, OXA, CTX-M, TEM-1, NDM-1, VIM-1, ere(A), ermA, ermB, ermC, msrA, qacA, qacB, and qacC.
Prevalence and Characteristics of Invasive Staphylococcus argenteus among Patients with Bacteremia in Hong Kong
Authors: Chen Jonathan H. K. , Leung Hoi-Yi , Wong Charles M. C. , Yuen Kwok-Yung , Cheng Vincent C. C.
Published: September 2023

Staphylococcus argenteus is a novel Staphylococcus species derived from Staphylococcus aureus. Information on the prevalence and genetic characteristics of invasive S. argenteus in Asia is limited. In this study, 275 invasive S. aureus complex strains were retrieved from blood culture specimens in Hong Kong and re-analyzed using MALDI-TOF mass spectrometry and an in-house multiplex real-time PCR for S. argenteus. The prevalence of invasive S. argenteus in Hong Kong was found to be 4.0% (11/275). These strains were primarily susceptible to commonly used antibiotics, except penicillin. Whole-genome sequencing revealed the circulation of three S. argenteus genotypes (ST-2250, ST-1223, and ST-2854) in Hong Kong, with ST-2250 and ST-1223 being the predominant genotypes. The local ST-2250 and ST-1223 strains showed close phylogenetic relationships with isolates from mainland China. Antimicrobial-resistant genes (fosB, tet-38, mepA, blaI, blaZ) could be found in nearly all local S. argenteus strains. The ST-1223 and ST-2250 genotypes carried multiple staphylococcal enterotoxin genes that could cause food poisoning and toxic shock syndrome. The CRISPR/Cas locus was observed only in the ST-2250 strains. This study provides the first report on the molecular epidemiology of invasive S. argenteus in Hong Kong, and further analysis is needed to understand its transmission reservoir.
Changes in Physicochemical Properties of Coal and Their Mechanism Due to Supercritical CO2–H2O Treatment
Authors: Chen Run , Zhang Yajun , Hu Kunpeng , Tu Guanglong , Dou Tianzheng
Published: September 2023

The dissolution of supercritical carbon dioxide (ScCO2) in water forms a ScCO2–H2O system, which exerts a transformative influence on the physicochemical characteristics of coal and significantly impacts the CO2-driven enhanced coalbed methane (CO2-ECBM) recovery process. Herein, the effect of ScCO2–H2O treatment on the physicochemical properties of coal was simulated in a high-pressure reactor. The migration of major elements, change in the pore structure, and change in the CH4 adsorption capacity of coal after the ScCO2–H2O treatment were detected using plasma emission spectroscopy, the low-temperature liquid nitrogen adsorption method, and the CH4 adsorption method, respectively. The results show that (1) the ScCO2–H2O treatment led to mineral reactions causing a significant migration of constant elements in the coal. The migration of Ca ions was the most significant, with an increase in their concentration in treated water from 0 to 16–970 mg·L−1, followed by Na, Mg, and K. Al migrated the least, from 0 to 0.004–2.555 mg·L−1. (2) The ScCO2–H2O treatment increased the pore volume and pore-specific surface area (SSA) of the coal via the dissolution and precipitation of minerals in the coal pores. The total pore volume increased from 0.000795–0.011543 to 0.001274–0.014644 cm3·g−1, and the total pore SSA increased from 0.084–3.332 to 0.400–6.061 m2·g−1. (3) Changes in the CH4 adsorption capacity were affected by the combined effects of a mineral reaction and pore structure change. The dissolved precipitates of the minerals in the coal pores after the ScCO2–H2O treatment caused elemental migration, which not only decreased the mineral content in the coal pores but also increased the total pore volume and total pore SSA, thus improving the CH4 adsorption capacity of the coal. This study provides theoretical support for CO2 sequestration and ECBM recovery.
Mineral Prospectivity Mapping for Orogenic Gold Mineralization in the Rainy River Area, Wabigoon Subprovince
Authors: Behnia Pouran , Harris Jeff , Sherlock Ross , Naghizadeh Mostafa , Vayavur Rajesh
Published: September 2023

Random Forest classification was applied to create mineral prospectivity maps (MPM) for orogenic gold in the Rainy River area of Ontario, Canada. Geological and geophysical data were used to create 36 predictive maps as RF algorithm input. Eighty-three (83) orogenic gold prospects/occurrences were used to train the classifier, and 33 occurrences were used to validate the model. The non-Au (negative) points were randomly selected with or without spatial restriction. The prospectivity mapping results show high performance for the training and test data in area-frequency curves. The F1 accuracy is high and moderate when assessed with the training and test data, respectively. The mean decrease accuracy was applied to calculate the variable importance. Density, proximity to lithological contacts, mafic to intermediate volcanics, analytic signal, and proximity to the Cameron-Pipestone deformation zone exhibit the highest variable importance in both models. The main difference between the models is in the uncertainty maps, in which the high-potential areas show lower uncertainty in the maps created with spatial restriction when selecting the negative points.
Benzophenanthridine Alkaloid Chelerythrine Elicits Necroptosis of Gastric Cancer Cells via Selective Conjugation at the Redox Hyperreactive C-Terminal Sec498 Residue of Cytosolic Selenoprotein Thioredoxin Reductase
Authors: Liu Minghui , Sun Shibo , Meng Yao , Wang Ling , Liu Haowen , Shi Wuyang , Zhang Qiuyu , Xu Weiping , Sun Bingbing , Xu Jianqiang
Published: September 2023

Targeting thioredoxin reductase (TXNRD) with low-weight molecules is emerging as a high-efficacy anti-cancer strategy in chemotherapy. Sanguinarine has been reported to inhibit the activity of TXNRD1, indicating that benzophenanthridine alkaloid is a fascinating chemical entity in the field of TXNRD1 inhibitors. In this study, the inhibition of three benzophenanthridine alkaloids, including chelerythrine, sanguinarine, and nitidine, on recombinant TXNRD1 was investigated, and their anti-cancer mechanisms were revealed using three gastric cancer cell lines. Chelerythrine and sanguinarine are more potent inhibitors of TXNRD1 than nitidine, and the inhibitory effects take place in a dose- and time-dependent manner. Site-directed mutagenesis of TXNRD1 and in vitro inhibition analysis proved that chelerythrine or sanguinarine is primarily bound to the Sec498 residue of the enzyme, but the neighboring Cys497 and remaining N-terminal redox-active cysteines could also be modified after the conjugation of Sec498. With high similarity to sanguinarine, chelerythrine exhibited cytotoxic effects on multiple gastric cancer cell lines and suppressed the proliferation of tumor spheroids derived from NCI-N87 cells. Chelerythrine elevated cellular levels of reactive oxygen species (ROS) and induced endoplasmic reticulum (ER) stress. Moreover, the ROS induced by chelerythrine could be completely suppressed by the addition of N-acetyl-L-cysteine (NAC), and the same is true for sanguinarine. Notably, Nec-1, an RIPK1 inhibitor, rescued the chelerythrine-induced rapid cell death, indicating that chelerythrine triggers necroptosis in gastric cancer cells. Taken together, this study demonstrates that chelerythrine is a novel inhibitor of TXNRD1 by targeting Sec498 and possessing high anti-tumor properties on multiple gastric cancer cell lines by eliciting necroptosis.
Sea Conch Peptides Hydrolysate Alleviates DSS-Induced Colitis in Mice through Immune Modulation and Gut Microbiota Restoration
Authors: Ullah Hidayat , Deng Ting , Ali Muhsin , Farooqui Nabeel Ahmed , Alsholi Duaa M. , Siddiqui Nimra Zafar , Rehman Ata Ur , Ali Sharafat , Ilyas Muhammad , Wang Liang , Xin Yi
Published: September 2023

Inflammatory bowel disease (IBD) is a persistent, lifelong inflammation of the digestive system. Dextran sulfate sodium is commonly used to induce colitis in experimental animal models, which causes epithelial damage, intestinal inflammation, mucin depletion, and dysbiosis of the gut microbiota. Various prebiotics, polysaccharides, and polypeptides are used for IBD treatment. In this study, we used a murine model utilizing BALB/c mice, with 10 mice per group, to investigate the treatment effect of sea conch peptide hydrolysate (CPH) on DSS-induced colitis mice. Colitis was induced through the administration of 2.5% DSS in drinking water over a seven-days period. Furthermore, on the eighth day of the experiment, sea conch peptide hydrolysate (CPH) at low (100 mg/kg), medium (200 mg/kg), and high (400 mg/kg) doses, which were continued for 14 days, were assessed for medicinal purposes in DSS-induced colitis mice. Our results showed that CPH treatment significantly alleviated the severity and symptoms of colitis. The epithelial integrity and histological damage were improved. Intestinal inflammation and inflammatory cell infiltration were improved. Furthermore, the expression of pro-inflammatory cytokines was reduced, and intestinal barrier integrity was restored by elevating the tight junction proteins. Moreover, 16s RNA sequencing revealed dysbiosis of the gut microbiota was observed upon DSS treatment, which was reinstated after CPH treatment. An increased level of Firmicutes and Lactobacillus was observed in the treatment groups. Finally, our results suggest that CPH would be recommended as a functional food source and also have the potential to be used as a medicinal product for different gastrointestinal disorders.
The Controllable Mechanical Properties of Coiled Carbon Nanotubes with Stone–Wales and Vacancy Defects
Authors: Bie Zhiwu , Deng Yajie , Liu Xuefeng , Zhu Jiaqi , Tao Jixiao , Shi Xian , He Xiaoqiao
Published: September 2023

Coiled carbon nanotubes (CCNTs) as a promising nanometer scale spring are investigated for the effect of the defects on the tensile mechanical properties of CCNTs by using molecular dynamics (MD) simulations. Six samples of defective CCNTs are constructed by introducing the defects in the different positions. The results show an obvious decrease in the spring constant and elastic limit of defective CCNTs, which results in the lower energy storage ability during the elastic range compared with the perfect CCNTs. However, the defected CCNTs exhibit better ductility (138.9%) and higher energy absorbing ability (1539.93 J/g) during the fracture process since introduced defects change the deformation pattern. Furthermore, among the defected CCNTs, the stiffness (1.48~1.93 nN/nm), elastic limit (75.2~88.7%), ductility (108.5~138.9%), and deformation pattern can be adjusted by changing the position or the type of defects. This study firstly provides insight into the effects of Stone–Wales (SW) and vacancy defects on the mechanical properties of CCNTs, and the obtained results are meaningful for designing CCNTs with specified properties by introducing defects.
Instability of Liquid Film with Odd Viscosity over a Non-Uniformly Heated and Corrugated Substrate
Authors: Xue Danting , Zhang Ruigang , Liu Quansheng , Ding Zhaodong
Published: September 2023

The effect of odd viscosity on the instability of liquid film along a wavy inclined bottom with linear temperature variation is investigated. By utilizing the long-wave approximation, the non-linear evolution equation of the free surface is derived. By applying the normal mode method, the linear instability of thin film flow is investigated. With the help of multi-scale analysis methods, the weakly non-linear instability of thin film flow is also investigated. The results reveal that the Marangoni effect caused by non-uniform temperature distribution promotes the instability of the liquid film, while the odd viscosity has a stabilizing effect. In addition, for a positive local inclination angle θ, an increase in bottom steepness ζ inhibits the instability of the liquid film flow. In contrast, with a negative local inclination angle θ, increased bottom steepness ζ promotes the instability of the liquid film flow. The results of the temporal linear instability analysis and the weakly non-linear instability analysis have been substantiated through numerical simulations of the non-linear evolution equations.
Fabrication of Hierarchical MOF-Derived NiCo2S4@Mo-Doped Co-LDH Arrays for High-Energy-Density Asymmetric Supercapacitors
Authors: Cheng Siyi , Du Kang , Wang Xiaowu , Han Yufei , Li Longxiao , Wen Guojun
Published: September 2023

The rational fabrication of composite structures made of mixed components has shown great potential for boosting the energy density of supercapacitors. Herein, an elaborate hierarchical MOF-derived NiCo2S4@Mo-doped Co-LDH arrays hybrid electrode was fabricated through a step-wise method. By leveraging the synergistic effects of a uniform array of NiCo2S4 nanowires as the core and an MOF-derived porous shell, the NiCo2S4@Mo-doped Co-LDH hybrid electrode demonstrates an exceptional specific capacitance of 3049.3 F g−1 at 1 A g−1. Even at a higher current density of 20 A g−1, the capacitance remains high at 2458.8 F g−1. Moreover, the electrode exhibits remarkable cycling stability, with 91% of the initial capacitance maintained after 10,000 cycles at 10 A g−1. Additionally, the as-fabricated asymmetric supercapacitor (ASC) based on the NiCo2S4@Mo-doped Co-LDH electrode achieves an impressive energy density of 97.5 Wh kg−1 at a power density of 835.6 W kg−1. These findings provide a promising approach for the development of hybrid-structured electrodes, enabling the realization of high-energy-density asymmetric supercapacitors.
Fabrication and Characterization of a Poly(3,4-ethylenedioxythiophene)@Tungsten Trioxide–Graphene Oxide Hybrid Electrode Nanocomposite for Supercapacitor Applications
Authors: Memou Cherifa Hakima , Bekhti Mohamed Amine , Kiari Mohamed , Benyoucef Abdelghani , Alelyani Magbool , Alqahtani Mohammed S. , Alshihri Abdulaziz A. , Bakkour Youssef
Published: September 2023

With the rapid development of nanotechnology, the study of nanocomposites as electrode materials has significantly enhanced the scope of research towards energy storage applications. Exploring electrode materials with superior electrochemical properties is still a challenge for high-performance supercapacitors. In the present research article, we prepared a novel nanocomposite of tungsten trioxide nanoparticles grown over supported graphene oxide sheets and embedded with a poly(3,4-ethylenedioxythiophene) matrix to maximize its electrical double layer capacitance. The extensive characterization shows that the poly(3,4-ethylenedioxythiophene) matrix was homogeneously dispersed throughout the surface of the tungsten trioxide–graphene oxide. The poly(3,4-ethylenedioxythiophene)@tungsten trioxide–graphene oxide exhibits a higher specific capacitance of 478.3 F·g−1 at 10 mV·s−1 as compared to tungsten trioxide–graphene oxide (345.3 F·g−1). The retention capacity of 92.1% up to 5000 cycles at 0.1 A·g−1 shows that this ternary nanocomposite electrode also exhibits good cycling stability. The poly(3,4-ethylenedioxythiophene)@tungsten trioxide–graphene oxide energy density and power densities are observed to be 54.2 Wh·kg−1 and 971 W·kg−1. The poly(3,4-ethylenedioxythiophene)@tungsten trioxide–graphene oxide has been shown to be a superior anode material in supercapacitors because of the synergistic interaction of the poly(3,4-ethylenedioxythiophene) matrix and the tungsten trioxide–graphene oxide surface. These advantages reveal that the poly(3,4-ethylenedioxythiophene)@tungsten trioxide–graphene oxide electrode can be a promising electroactive material for supercapacitor applications.
Probing the Nano-Assembly Leading to Periodic Gratings in Poly(p-dioxanone)
Authors: Hao Min-Han , Nagarajan Selvaraj , Woo Eamor M.
Published: September 2023

This study used scanning electron microscopy via 3D dissection coupled with synchrotron radiation with microfocal beams of both small-angle X-ray scattering and wide-angle X-ray diffraction to analyze the periodic crystal aggregates of unusual poly(p-dioxanone) (PPDO) dendritic cactus-arm-like ring bands upon crystallization with a diluent poly(vinyl alcohol) (PVA) that is capable of hydrogen bonding interactions with PPDO. Three-dimensional microscopy interior dissection clearly expounds that the banded periodic architectures are packed by alternately normal-oriented flat-on crystals underneath the valley, periodically interfaced/branched with horizontal-oriented edge-on fibrils underneath the ridge. The oblique angles between the valley’s flat-on crystals with the branches are ca. 25–45° (depending on gradient inclines and bending), which is also proved by the azimuthal angle in microbeam X-ray diffraction. The grating-like strut-rib assembly in the PPDO cactus-arm-like ring bands is further proved by novel iridescence tests.
Current Nursing Education Considering Southern Europe’s Reality and Legal Framework: A Two-Phased Research Approach
Authors: Antão Celeste , Antolí-Forner Aloma , Fernandes Hélder , Alves Sara Brás , Pimentel Helena
Published: September 2023

Nursing education and formation is still heterogenous in spite of The Bologna Declaration in 1999. Apart from the existence of basic curriculum standards, universities have flexibility regarding its design. The objective of this study is to provide an overview of contemporary nursing education and contrast it with the legal frameworks in place in four Southern European countries: Portugal, Spain, Italy, and Greece. A scoping review was conducted in order to revise and obtain an up-to-date review of current nursing education and quality. Then, a content evaluation of the legal public framework was conducted. A total of nine articles were included in the review. Data analysis evoked three main themes from the results: nursing education heterogeneity owing to ECTS increased from 180 to 240 for the diversity of clinical practice hours; the nursing framework lacked a definition; and the evolution of nursing education, alongside quality improvement and more accurate guidelines were required. Regarding their legal framework, the main directives and legislation standards were assessed and compared with the current curriculum. To conclude, nursing heterogeneity education evokes competence inequality among students and future professionals as larger curricular programs (240 ECTS) offer more clinical practice. Nursing education uniformity could enhance international mobility and promote knowledge exchange and nursing competence framework definitions. These are facts that certainly bring nursing empowerment. This study was not registered.
The Cytokine Profile in Different Stages of Schistosomiasis Japonica
Authors: Wang Xi , Tang Qi , Bergquist Robert , Zhou Xiaorong , Qin Zhiqiang
Published: September 2023

To explore and profile the level of cytokines in the sera of patients infected with Schistosoma japonicum to explore the helper T-cell response of patients either at the chronic or advanced stage of the disease. We randomly selected 58 subjects from several areas endemic for schistosomiasis japonica in China and collected serum samples to be tested for 18 different cytokines secreted by (1) Th1/Th2 cells (GM-CSF, IFN-γ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-12p70, IL-10, IL-13, IL-18 and TNF-α) and (2) Th9/Th17/Th22/Treg cells (IL-9, IL-17A, IL-21, IL-22, IL-23 and IL-27). The Th1/Th2 cytokines in chronic patients were not significantly different from those in healthy people, while patients with advanced schistosomiasis had higher levels of IL-2, IL-23 and IL-27 and lower levels of IL-18 and IFN-γ. With respect to the Th9/Th17/Th22/Treg cell cytokines, there were higher levels of IL-23. Thus, a limited variation of the cytokine response between the three patient groups was evident, but only in those with advanced infection, while there was no difference between chronic schistosomiasis infection and healthy subjects in this respect. The cytokine expression should be followed in patients with advanced schistosomiasis who show a cytokine pattern of a weakened Th1 cell response and an increased Th17 response.
Controlling Plasmon Resonance of Gold and Silver Nanoparticle Arrays with Help of Liquid Crystal
Authors: Yakovkin Ivan , Reshetnyak Victor
Published: September 2023

The tunability of plasmonic resonances in gold and silver nanosphere arrays on a glass substrate, embedded in a liquid crystal matrix, was explored. The calculations involving the finite element method revealed that the optical properties of these arrays can be modulated by reorienting the liquid crystal. When the liquid crystal director was reoriented between planar and homeotropic configurations in the plane containing the incident wave polarization vector, the plasmonic resonance wavelength shifted within an approximately 100 nm range. A reduced shift of about 40 nm was observed when the reorientation occurred in the plane perpendicular to the polarization. Both metal nanosphere arrays showed notable near-field amplification. Gold achieved up to 18 times the amplification of the incident wave electric field, while silver reached 16 times but showed a remarkable 40 times amplification at the inter-band transition resonance wavelength. This research underscores the potential of using liquid crystal reorientation for controlling the plasmonic lattice resonance in metal nanosphere arrays, opening up new possibilities for adaptable plasmonic devices.
In-Situ Detection for Atomic Density in the K-Rb-21Ne Co-Magnetometer via an Optical Heterodyne Interferometry
Authors: Liu Sixun , Wang Zhuo , Zhai Yueyang
Published: September 2023

The low-frequency fluctuations of the atomic density within the cell can induce the longterm drift of the K-Rb-21Ne spin-exchange relaxation-free (SERF) co-magnetometer output, such that the accurate measurement of in situ atomic density is of great significance for improving the performance of co-magnetometer. In this paper, the complex refractive index model of the spin ensembles under the hybrid optical pumping condition is established first, according to which the relation between atomic density and its complex refractive index is revealed and an optical heterodyne-based scheme for atomic density detection is proposed. The dependence of the atomic density on the demodulated phase signal from the optical heterodyne-based scheme is provided by numerical simulations. After that, a dual acousto-optics frequency shifter (AOFS)-based optical heterodyne interferometry is constructed with a noise level below 1 mrad/Hz for frequencies > 1 Hz, and a compact SERF co-magnetometer is implemented as the testing medium, by which the atomic density detection with resolution of 0.40 K @ 473 K is reached and the experimental results agree well with theoretical simulations. Moreover, the detection scheme proposed in this paper has the properties of high detection sensitivity and immunity to laser power fluctuation, which are also proved experimentally.
The Hawking Effect in the Particles–Partners Correlations
Authors: Balbinot Roberto , Fabbri Alessandro
Published: September 2023

We analyze the correlations functions across the horizon in Hawking black hole radiation to reveal the correlations between Hawking particles and their partners. The effects of the underlying space–time on this are shown in various examples ranging from acoustic black holes to regular black holes.
Development of Pinaceae and Cupressaceae Essential Oils from Forest Waste in South Korea
Authors: Park Chanjoo , Woo Heesung , Park Mi-Jin
Published: September 2023

The growing awareness of environmental issues has garnered increasing interest in the use of waste material in a wide variety of applications. From this viewpoint, developing essential oils from forest waste can bring new cost opportunities for the effective and sustainable management of unused forestry biomass. However, better knowledge of the production, chemical constituents, and application of essential oils is necessary. Among the species considered to be of interest from the point of view of their essential oils and forest biomass, Pinaceae and Cupressaceae were selected in this study as potential candidates for commercial essential oils based on previous studies. This current study focuses on investigating Pinaceae (Larix kaempferi, Pinus densiflora, and Pinus koraiensis) and Cupressaceae (Chamaecyparis obtusa and Chamaecyparis pisifera) essential oils extracted from various parts from the perspective of their bioactive compounds and potential applications. This is followed by an overview of the essential oils industry in South Korea, with particular attention being paid to utilising unused forest biomass. Therefore, this is a comprehensive review suggesting that Pinaceae and Cupressaceae essential oils extracted from various parts of forest waste could be utilised in various industries, adding value to the aspect of sustainable industry. Furthermore, our study contributes towards capturing the value of forest resources through the utilisation of native essential oils in South Korea.
Moroccan Citrus clementina Peels: Optimization of Pectin Extraction and Determination of Chemical and Functional Properties
Authors: Azzouzi Hanane , Elhajji Loubna , Achchoub Mouad , Salmaoui Souad , Ammadi Abdelillah , Harrak Hasnaa , Touzani Rachid , Noutfia Younes , Elfazazi Kaoutar
Published: September 2023

Citrus peels are considered a rich source of valuable biomolecules. Pectin is a polymer of polysaccharide acid and is composed of galacturonic acid monosaccharides. In this study, response surface methodology was used to optimize pectin extraction from Citrus × clementina Hort. ex Tan. (Rutaceae) peels using citric acid as an extraction solvent. The effect of the parameters conditioning the extraction process and pectin yield (pH level, temperature, extraction time, solid/liquid ratio, and raw material particle size) was investigated using a Box–Behnken design. The quality of the extracted pectin was assessed both chemically (moisture, ash, protein, and carbohydrate content) and functionally (gelling power and emulsifying activity). According to the screening experiment, the pH level, temperature, and particle size were the main factors influencing the pectin yield. The adjusted mathematical model enabled us to plot response surfaces in order to determine the optimal extraction conditions. The highest production yield of pectin (26.6%) was obtained at the optimal conditions of pH = 1.5, temperature = 100 °C, and particle size = 0.1 mm for an extraction time of 30 min. Compared to the predicted value of 26.6%, the experimental extraction yield of C. clementina was about 21.4% of pectin. Concerning the functional properties, the extracted pectin had a high gelling power of 164 ° SAG and an emulsifying activity of 38.5%.
The Potential of Essential Oils from Active Packaging to Reduce Ethylene Biosynthesis in Plant Products. Part 2: Fruits (Blueberries and Blackberries)
Authors: López-Gómez Antonio , Navarro-Martínez Alejandra , Garre Alberto , Iguaz Asunción , Martínez-Hernández Ginés Benito
Published: September 2023

Plant essential oils (EOs) have an important ability to inhibit ethylene biosynthesis. Nevertheless, the effects of EOs on the key components of ethylene biosynthesis (l-aminocyclopropane-1-carboxylic (ACC) oxidase activity, ACC synthase activity, and ACC content) have not yet been thoroughly studied. Accordingly, this study focused on the effects of emitted EOs from active packaging (EO doses from 100 to 1000 mg m−2) on the key components of ethylene biosynthesis of blueberries and blackberries under several storage temperatures. Anise EO and lemon EO active packaging induced the greatest inhibitory effects (60–76%) on the ethylene production of blueberries and blackberries, respectively, even at high storage temperatures (22 °C). In terms of EO doses, active packaging with 1000 mg m−2 of anise EO or lemon EO led to the highest reduction of ethylene production, respectively. At 22 °C, the investigated EO active packing reduced the activities of ACC synthase and ACC oxidase up to 50%. In order to minimise ethylene biosynthesis in blueberries and blackberries when they are stored even under improper temperature scenarios at high temperatures, this EO active packaging is a natural and efficient technological solution.
Is Hydrogen Peroxide Generated in Infusions of Medicinal Herbs?
Authors: Tama Anna , Pieńkowska Natalia , Stefaniuk Ireneusz , Bartosz Grzegorz , Kapusta Ireneusz , Sadowska-Bartosz Izabela
Published: September 2023

Hydrogen peroxide is an important intra- and inter-cellular messenger. It has been reported that beverages such as tea and coffee generate H2O2. The aim of this study was to examine whether H2O2 is formed in infusions of medicinal herbs and to explain the mechanism of its formation. Infusions of sixteen herbs were studied and the presence of H2O2 was found in all cases. The highest concentrations of H2O2, exceeding 50 µM, were found in the infusions of leaves of Betula and Polygonum, as well as of the inflorescence of Tilia. Considerable amounts of diverse phenolic compounds were detected in the infusions. Two successive one-electron oxidations of phenolics are the main source of H2O2 in these infusions; as expected, the generation of semiquinone and superoxide radicals was confirmed. The infusions of medicinal herbs were also able to scavenge H2O2. The herbal infusions were cytotoxic to human ovary cancer SKOV-3 and PEO1 cells; this cytotoxicity was compromised by catalase added to the growth medium, demonstrating the contribution of H2O2 to the cytotoxic action of herbal extracts.
In Vitro and In Silico Studies of Antimicrobial Saponins: A Review
Authors: Li Jinhui , Monje-Galvan Viviana
Published: September 2023

Antibiotics are important drugs for the treatment of microbial infections and related diseases. However, due to the abuse of antibiotics, drug resistance has become a serious and urgent problem. The development of new antibiotics is a crucial area of research, and natural products are one of the main sources of novel antibiotics. Among various potential natural antimicrobial products, saponins attracted much attention due to their excellent and broad-spectrum antimicrobial properties. Although there are several reviews on antibacterial saponins, this review is the first to highlight the potential antibacterial mechanisms of saponins from both experimental and molecular simulation perspectives to provide a comprehensive panorama of the field. This review presents the current progress in the development and repurposing of natural-product antibiotics. The focus is centered on antimicrobial saponins discovered in recent years as well as the synergistic effect of some saponins with traditional antibiotics. This review presents experimental and simulation studies in this field to provide a multiscale overview of the antimicrobial mechanisms of saponins and potential directions for future research.
Nutraceutical and Functional Properties of Lupin Protein Extracts Obtained via a Combined Ultrasonication and Microwave-Assisted Process
Authors: Rababah Taha , Al Udatt Muhammad , Angor Malak , Gammoh Sana , Almahasneh Majdi , Magableh Ghazi , Abu Kayed Aseel , Almajwal Ali , AL-Rayyan Numan
Published: September 2023

Lupin is an agriculturally economical plant seed. It is considered a rich source of protein for both humans and animals in several regions of the world. Lupin proteins also could act as an encapsulation agent for vitamins and other nutritional factors after extraction. This research evaluated the effects of microwave and ultrasound extraction methods on sweet and bitter lupin proteins intended to act as encapsulation agents. Two distinct sizes of nanoparticles were detected using different types of treatments. Bitter lupin was optimal for generating large nanoparticles, while sweet lupin was optimum for generating small nanoparticles. Ultrasound treatment of bitter lupin after microwave treatment hydrolyzed some of the β-conglutin, which suggested a method to reduce the potential allergic reactions caused by lupin products. The total phenolic content and alpha-amylase inhibitory effect of sweet and bitter lupin proteins was high. Furthermore, the microwave extraction method showed the benefits of reduced solvent consumption and less time. The current results showed a positive effect of the combined application of ultrasound-assisted and microwave-assisted processes on the nutraceutical and functional properties of lupin protein extracts.
Computational Assessment of ZrO2-Al2O3/EG and ZrO2-Al2O3-Cu/EG Nanomaterial on Blasius-Rayleigh-Stokes Flow Influenced by an Aligned Magnetic Field
Authors: Alfannakh Huda , Souayeh Basma
Published: September 2023

In this work, the flow of a modified nanofluid is analysed as it passes over a moving surface to investigate the influence of nonlinear radiative heat transfer and the effects of magnetic fields that are aligned. In addition, ethylene glycol is used as the solvent while zirconium oxide and alumina are combined to generate a hybrid nanomaterial. Ternary nanomaterials consist of zirconium oxide, alumina, and copper dissolved in the ethylene glycol. For this mathematical model, Navier–Stokes equations were used to represent the assumed flow. The Navier–Stokes equations were approximated using the boundary layer method under the flow assumptions, yielding the PDE’s. Similarity transformations are used to translate this system into ODE’s. The bvp4c method is used to explain a dimensionless system. The impacts of the relevant physical parameters are elucidated quantitatively and visually. A greater temperature ratio parameter is observed to increase the temperature profile. In addition to this, when the magnetic field parameter is increased, the momentum layer becomes thicker.