bluuebunny/crossref_metadata_2025 huggingface dataset:
Feedback Linearization and Reaching Law Based Sliding Mode Control Design for Nonlinear Hydraulic Turbine Governing System
Authors: Guo Bicheng , Guo Jiang
Published: June 2019

Hydropower as renewable energy has continually expanded at a relatively high rate in the last decade. This expansion calls for more accurate scheme design in hydraulic turbine governing system (HTGS) to ensure its high efficiency. Sliding mode control (SMC) as a robust control method which is insensitive to system uncertainties and disturbances raises interest in the application in HTGS. However, the feature of highly coupled state variables reflects the nonlinear essence of HTGS and SMC studies on the related mathematical model under certain fluctuations are not satisfied. In this regard, a novel SMC design with proportional-integral-derivative manifold is firstly applied to a nonlinear HTGS with a complex conduit system. In dealing with certain fluctuations in speed and load around the rated working condition, the proposed SMC is capable of driving the system to the desired state with smooth and light responses in aspects of the key state variables. The exponential reaching law and introduced boundary layer fasten the speed of converging time and suppress chattering. A necessary integral of sliding parameter added to manifold successfully reduces the latency caused by the anti-regulation feature of HTGS. Three operating scenarios are simulated compared with the PSO-PID method, and results imply that the proposed SMC method equips with accurate trajectory tracking ability and smooth responses. Finally, the strong robustness against system uncertainties is tested.
Time Series Irradiance Synthesizer for Optical GEO Satellite Downlinks in 5G Networks
Authors: Kapsis Theodore T. , Lyras Nikolaos K. , Kourogiorgas Charilaos I. , Panagopoulos Athanasios D.
Published: June 2019

Next generation 5G networks generate a need for broadband, low latency and power efficient backhauling and data-relay services. In this paper, optical satellite communications links, as an integrated component of 5G networks, are studied. More specifically, the Geostationary (GEO) satellite-to-ground optical communication link is investigated. Long-term irradiance statistics based on experimental measurements from the ARTEMIS program are presented and a new time series generator related to the received irradiance/power fluctuations due to atmospheric turbulence is reported. The proposed synthesizer takes into consideration the turbulence-induced scintillation effects that deteriorate the laser beam propagation, on the assumption of the Kolmogorov spectrum. The modeling is based on Rytov theory regarding weak turbulence conditions with the incorporation of first order stochastic differential equations. Summing up, the time series synthesizer is validated in terms of first and second order statistics with experimental results from the European Space Agency‘s ARTEMIS experimental optical downlink and simulated received power statistics for various weather conditions are presented using the proposed validated methodology. Some important conclusions are drawn.
Interplay of Sensor Quantity, Placement and System Dimension in POD-Based Sparse Reconstruction of Fluid Flows
Authors: Jayaraman Balaji , Al Mamun S M Abdullah , Lu Chen
Published: June 2019

Sparse linear estimation of fluid flows using data-driven proper orthogonal decomposition (POD) basis is systematically explored in this work. Fluid flows are manifestations of nonlinear multiscale partial differential equations (PDE) dynamical systems with inherent scale separation that impact the system dimensionality. Given that sparse reconstruction is inherently an ill-posed problem, the most successful approaches require the knowledge of the underlying low-dimensional space spanning the manifold in which the system resides. In this paper, we adopt an approach that learns basis from singular value decomposition (SVD) of training data to recover sparse information. This results in a set of four design parameters for sparse recovery, namely, the choice of basis, system dimension required for sufficiently accurate reconstruction, sensor budget and their placement. The choice of design parameters implicitly determines the choice of algorithm as either l 2 minimization reconstruction or sparsity promoting l 1 minimization reconstruction. In this work, we systematically explore the implications of these design parameters on reconstruction accuracy so that practical recommendations can be identified. We observe that greedy-smart sensor placement, particularly interpolation points from the discrete empirical interpolation method (DEIM), provide the best balance of computational complexity and accurate reconstruction.
Single Nucleotide Polymorphisms of NUCB2 and Their Genetic Associations with Milk Production Traits in Dairy Cows
Authors: Han Bo , Yuan Yuwei , Li Yanhua , Liu Lin , Sun Dongxiao
Published: June 2019

We previously used the RNA sequencing technique to detect the hepatic transcriptome of Chinese Holstein cows among the dry period, early lactation, and peak of lactation, and implied that the nucleobindin 2 (NUCB2) gene might be associated with milk production traits due to its expression being significantly increased in early lactation or peak of lactation as compared to dry period (q value < 0.05). Hence, in this study, we detected the single nucleotide polymorphisms (SNPs) of NUCB2 and analyzed their genetic associations with milk yield, fat yield, fat percentage, protein yield, and protein percentage. We re-sequenced the entire coding and 2000 bp of 5′ and 3′ flanking regions of NUCB2 by pooled sequencing, and identified ten SNPs, including one in 5′ flanking region, two in 3′ untranslated region (UTR), and seven in 3′ flanking region. The single-SNP association analysis results showed that the ten SNPs were significantly associated with milk yield, fat yield, fat percentage, protein yield, or protein percentage in the first or second lactation (p values <= 1 × 10−4 and 0.05). In addition, we estimated the linkage disequilibrium (LD) of the ten SNPs by Haploview 4.2, and found that the SNPs were highly linked in one haplotype block (D′ = 0.98–1.00), and the block was also significantly associated with at least one milk traits in the two lactations (p values: 0.0002–0.047). Further, we predicted the changes of transcription factor binding sites (TFBSs) that are caused by the SNPs in the 5′ flanking region of NUCB2, and considered that g.35735477C>T might affect the expression of NUCB2 by changing the TFBSs for ETS transcription factor 3 (ELF3), caudal type homeobox 2 (CDX2), mammalian C-type LTR TATA box (VTATA), nuclear factor of activated T-cells (NFAT), and v-ets erythroblastosis virus E26 oncogene homolog (ERG) (matrix similarity threshold, MST > 0.85). However, the further study should be performed to verify the regulatory mechanisms of NUCB2 and its polymorphisms on milk traits. Our findings first revealed the genetic effects of NUCB2 on the milk traits in dairy cows, and suggested that the significant SNPs could be used in genomic selection to improve the accuracy of selection for dairy cattle breeding.
Significant Cellular Viability Dependence on Time Exposition at ELF-EMF and RF-EMF In Vitro Studies
Authors: García-Minguillán López Olga , Jiménez Valbuena Ana , Maestú Unturbe Ceferino
Published: June 2019

The human concern about the effect of electromagnetic fields (EMFs) has changed over time from the effects produced by EMFs of extremely low frequencies (ELFs) to the effects produced by exposure to a radio frequency (RF), with concerns shifting toward EMFs due to the development of new technologies and forms of communication. Previous studies have analysed the effects produced at different frequencies without considering in detail the effect of the time of exposure. Therefore, in the present study, we analysed in vitro the effect produced by a 100 µT EMF at different ELFs and exposure times in glioblastomas, as well as the effect produced in a fibroblast by an RF-EMF of 2.54 GHz. Our results indicate a significant time dependence in cell viability of fibroblasts exposed to an RF-EMF of 2.54 GHz and a non-time-dependent effect in cell viability of glioblastomas exposed to an ELF-EMF, highlighting the possible relation between frequency and time of exposure.
Personalized Moderate-Intensity Exercise Training Combined with High-Intensity Interval Training Enhances Training Responsiveness
Authors: Byrd Bryant R. , Keith Jamie , Keeling Shawn M. , Weatherwax Ryan M. , Nolan Paul B. , Ramos Joyce S. , Dalleck Lance C.
Published: June 2019

This study sought to determine if personalized moderate-intensity continuous exercise training (MICT) combined with high-intensity interval training (HIIT) was more effective at improving comprehensive training responsiveness than MICT alone. Apparently healthy, but physically inactive men and women (n = 54) were randomized to a non-exercise control group or one of two 13-week exercise training groups: (1) a personalized MICT + HIIT aerobic and resistance training program based on the American Council on Exercise guidelines, or (2) a standardized MICT aerobic and resistance training program designed according to current American College of Sports Medicine guidelines. Mean changes in maximal oxygen uptake (VO2max) and Metabolic (MetS) z-score in the personalized MICT + HIIT group were more favorable (p < 0.05) when compared to both the standardized MICT and control groups. Additionally, on the individual level, there were positive improvements in VO2max (Δ > 4.9%) and MetS z-score (Δ ≤ −0.48) in 100% (16/16) of participants in the personalized MICT + HIIT group. In the present study, a personalized exercise prescription combining MICT + HIIT in conjunction with resistance training elicited greater improvements in VO2max, MetS z-score reductions, and diminished inter-individual variation in VO2max and cardiometabolic training responses when compared to standardized MICT.
The Potential of Remedial Techniques for Hazard Reduction of Steel Process by Products: Impact on Steel Processing, Waste Management, the Environment and Risk to Human Health
Authors: Rodgers Kiri , McLellan Iain , Cuthbert Simon , Masaguer Torres Victoria , Hursthouse Andrew
Published: June 2019

The negative impact from industrial pollution of the environment is still a global occurrence, and as a consequence legislation and subsequent regulation is becoming increasingly stringent in response, in particular, to minimising potential impact on human health. These changes have generated growing pressures for the steel industry to innovate to meet new regulations driving a change to the approach to waste management across the industrial landscape, with increasing focus on the principles of a circular economy. With a knowledge of the compositional profiles of process by-products, we have assessed chemical cleaning to improve environmental performance and minimise disruption to manufacturing processes, demonstrating re-use and recycling capacity. We show that with a knowledge of phase composition, we are able to apply stabilisation methods that can either utilise waste streams directly or allow manipulation, making them suitable for re-use and/or inert disposal. We studied blast furnace slags and Portland cement mixes (50%/50% and 30%/70%) with a variety of other plant wastes (electrostatic precipitator dusts (ESP), blast furnace (BF) sludge and basic oxygen furnace (BOF) sludge) which resulted in up to 90% immobilisation of hazardous constituents. The addition of organic additives i.e., citric acid can liberate or immobilise problematic constituents; in the case of K, both outcomes occurred depending on the waste type; ESP dust BF sludge and BOF fine sludge. Pb and Zn however were liberated with a 50–80% and 50–60% residue reduction respectively, which generates possibilities for alternative uses of materials to reduce environmental and human health impact.
Toward an Improved Air Pollution Warning System in Quebec
Authors: Masselot Pierre , Chebana Fateh , Lavigne Éric , Campagna Céline , Gosselin Pierre , Ouarda Taha B.M.J.
Published: June 2019

The nature of pollutants involved in smog episodes can vary significantly in various cities and contexts and will impact local populations differently due to actual exposure and pre-existing sensitivities for cardiovascular or respiratory diseases. While regulated standards and guidance remain important, it is relevant for cities to have local warning systems related to air pollution. The present paper proposes indicators and thresholds for an air pollution warning system in the metropolitan areas of Montreal and Quebec City (Canada). It takes into account past and current local health impacts to launch its public health warnings for short-term episodes. This warning system considers fine particulate matter (PM2.5) as well as the combined oxidant capacity of ozone and nitrogen dioxide (Ox) as environmental exposures. The methodology used to determine indicators and thresholds consists in identifying extreme excess mortality episodes in the data and then choosing the indicators and thresholds to optimize the detection of these episodes. The thresholds found for the summer were 31 μg/m3 for PM2.5 and 43 ppb for Ox in Montreal, and 32 μg/m3 and 23 ppb in Quebec City. In winter, thresholds found were 25 μg/m3 and 26 ppb in Montreal, and 33 μg/m3 and 21 ppb in Quebec City. These results are in line with different guidelines existing concerning air quality, but more adapted to the cities examined. In addition, a sensitivity analysis is conducted which suggests that Ox is more determinant than PM2.5 in detecting excess mortality episodes.
Chronic Viral Hepatitis Signifies the Association of Premixed Insulin Analogues with Liver Cancer Risks: A Nationwide Population-Based Study
Authors: Chiang Chien-Hsieh , Kuo Chia-Sheng , Lin Wan-Wan , Su Jun-Han , Chen Jin-De , Huang Kuo-Chin
Published: June 2019

This study sought to determine whether chronic hepatitis B or C would modify the association between insulin analogues and hepatocellular carcinoma (HCC) risks. We conducted a nationwide nested case-control study for HCC cases and matched controls from 2003 to 2013 among newly diagnosed type 2 diabetes patients on any antidiabetic agents in Taiwan before and after exclusion of chronic viral hepatitis, respectively. A total of 5832 and 1237 HCC cases were identified before and after exclusion of chronic viral hepatitis, respectively. Incident HCC risks were positively associated with any use of premixed insulin analogues (adjusted odds ratio (OR), 1.27; 95% CI 1.04 to 1.55) among total participants, especially among current users (adjusted OR, 1.45; 95% CI 1.12 to 1.89). However, the association between HCC occurrence and premixed insulin analogues diminished among participants without chronic viral hepatitis (adjusted OR, 1.35; 95% CI 0.92 to 1.98). We also observed a significant multiplicative interaction between chronic viral hepatitis and premixed insulin analogues on HCC risks (P = 0.010). Conclusions: Chronic viral hepatitis signifies the role of premixed insulin analogues in HCC oncogenesis. We recommend a closer liver surveillance among patients prescribed premixed insulin analogues with concomitant chronic viral hepatitis.
Work-Related Exposures and Sickness Absence Trajectories: A Nationally Representative Follow-up Study among Finnish Working-Aged People
Authors: Lallukka Tea , Kaila-Kangas Leena , Mänty Minna , Koskinen Seppo , Haukka Eija , Kausto Johanna , Leino-Arjas Päivi , Kaikkonen Risto , Halonen Jaana I. , Shiri Rahman
Published: June 2019

The contribution of physically demanding work to the developmental trajectories of sickness absence (SA) has seldom been examined. We analyzed the associations of 12 physical work exposures, individually and in combination, with SA trajectories among the occupationally active in the Finnish nationally representative Health 2000 survey. We included 3814 participants aged 30–59 years at baseline, when exposure history to work-related factors was reported. The survey and interview responses were linked with the annual number of medically confirmed SA spells through 2002–2008 from national registries. Trajectory analyses identified three SA subgroups: 1 = low (54.6%), 2 = slowly increasing (33.7%), and 3 = high (11.7%). After adjustments, sitting or use of keyboard >1 year was inversely associated with the high SA trajectory (odds ratio, OR, 0.57; 95% 95% confidence interval, CI, 0.43–0.77). The odds of belonging to the trajectory of high SA increased with an increasing number of risk factors, and was highest for those with ≥4 physical workload factors (OR 2.71; 95% CI 1.99–3.69). In conclusion, these findings highlight the need to find ways to better maintain the work ability of those in physically loading work, particularly when there occurs exposure to several workload factors.
Identification of a Retroelement-Containing Human Transcript Induced in the Nucleus by Vaccination
Authors: Honda Tomoyuki , Takemoto Keiko , Ueda Keiji
Published: June 2019

Endogenous retroelements constitute almost half of the mammalian genome. Given that more than 60% of human genomic bases are transcribed, transcripts containing these retroelements may impact various biological processes. However, the physiological roles of most retroelement-containing transcripts are yet to be revealed. Here, we profiled the expression of retroelement-containing human transcripts during vaccination and found that vaccination upregulated transcripts containing only particular retroelements, such as the MLT-int element of endogenous retroviruses. MLT-int-containing transcripts were distributed mainly in the nucleus, suggesting their unique roles in the nucleus. Furthermore, we demonstrated that MLT-int RNA suppressed interferon promoter activity in the absence of immune stimuli. Based on these lines of evidence, we speculate a model of a role of the previously unnoticed MLT-int element in preventing excess innate immune activation after elimination of immune stimuli. Our results may emphasize the importance of retroelement-containing transcripts in maintaining host immune homeostasis.
Why the Gold Standard Approach by Mammography Demands Extension by Multiomics? Application of Liquid Biopsy miRNA Profiles to Breast Cancer Disease Management
Authors: Zubor Pavol , Kubatka Peter , Kajo Karol , Dankova Zuzana , Polacek Hubert , Bielik Tibor , Kudela Erik , Samec Marek , Liskova Alena , Vlcakova Dominika , Kulkovska Tatiana , Stastny Igor , Holubekova Veronika , Bujnak Jan , Laucekova Zuzana , Büsselberg Dietrich , Adamek Mariusz , Kuhn Walther , Danko Jan , Golubnitschaja Olga
Published: June 2019

In the global context, the epidemic of breast cancer (BC) is evident for the early 21st century. Evidence shows that national mammography screening programs have sufficiently reduced BC related mortality. Therefore, the great utility of the mammography-based screening is not an issue. However, both false positive and false negative BC diagnosis, excessive biopsies, and irradiation linked to mammography application, as well as sub-optimal mammography-based screening, such as in the case of high-dense breast tissue in young females, altogether increase awareness among the experts regarding the limitations of mammography-based screening. Severe concerns regarding the mammography as the “golden standard” approach demanding complementary tools to cover the evident deficits led the authors to present innovative strategies, which would sufficiently improve the quality of the BC management and services to the patient. Contextually, this article provides insights into mammography deficits and current clinical data demonstrating the great potential of non-invasive diagnostic tools utilizing circulating miRNA profiles as an adjunct to conventional mammography for the population screening and personalization of BC management.
Epigenetic Alterations Induced by Photothrombotic Stroke in the Rat Cerebral Cortex: Deacetylation of Histone h3, Upregulation of Histone Deacetylases and Histone Acetyltransferases
Authors: Demyanenko Svetlana , Uzdensky Anatoly
Published: June 2019

Ischemic penumbra that surrounds a stroke-induced infarction core is potentially salvageable; however, mechanisms of its formation are not well known. Covalent modifications of histones control chromatin conformation, gene expression and protein synthesis. To study epigenetic processes in ischemic penumbra, we used photothrombotic stroke (PTS), a stroke model in which laser irradiation of the rat brain cortex photosensitized by Rose Bengal induces local vessel occlusion. Immunoblotting and immunofluorescence microscopy showed decrease in acetylation of lysine 9 in histone H3 in penumbra at 1, 4 or 24 h after PTS. This was associated with upregulation of histone deacetylases HDAC1 and HDAC2, but not HDAC4, which did not localize in the nuclei. HDAC2 was found in cell nuclei, HDAC4 in the cytoplasm and HDAC1 both in nuclei and cytoplasm. Histone acetyltransferases HAT1 and PCAF (p300/CBP associated factor) that acetylated histone H3 synthesis were also upregulated, but lesser and later. PTS increased localization of HDAC2 and HAT1 in astroglia. Thus, the cell fate in PTS-induced penumbra is determined by the balance between opposite tendencies leading either to histone acetylation and stimulation of gene expression, or to deacetylation and suppression of transcriptional processes and protein biosynthesis. These epigenetic proteins may be the potential targets for anti-stroke therapy.
Perspectives for Applying G-Quadruplex Structures in Neurobiology and Neuropharmacology
Authors: Asamitsu Sefan , Takeuchi Masayuki , Ikenoshita Susumu , Imai Yoshiki , Kashiwagi Hirohito , Shioda Norifumi
Published: June 2019

The most common form of DNA is a right-handed helix or the B-form DNA. DNA can also adopt a variety of alternative conformations, non-B-form DNA secondary structures, including the DNA G-quadruplex (DNA-G4). Furthermore, besides stem-loops that yield A-form double-stranded RNA, non-canonical RNA G-quadruplex (RNA-G4) secondary structures are also observed. Recent bioinformatics analysis of the whole-genome and transcriptome obtained using G-quadruplex–specific antibodies and ligands, revealed genomic positions of G-quadruplexes. In addition, accumulating evidence pointed to the existence of these structures under physiologically- and pathologically-relevant conditions, with functional roles in vivo. In this review, we focused on DNA-G4 and RNA-G4, which may have important roles in neuronal function, and reveal mechanisms underlying neurological disorders related to synaptic dysfunction. In addition, we mention the potential of G-quadruplexes as therapeutic targets for neurological diseases.
Expression Analysis Reveals the Association of Several Genes with Pupal Diapause in Bactrocera minax (Diptera: Tephritidae)
Authors: Wang Jia , Fan Huan , Wang Pan , Liu Ying-Hong
Published: June 2019

The Chinese citrus fly, Bactrocera minax, is a devastating pest of citrus, which enters the obligatory diapause in overwintering pupae to resist harsh environmental conditions. However, little is known about the molecular mechanisms underlying pupal diapause. The previous transcriptomic analysis revealed that a large number of genes were regulated throughout the pupal stage. Of these genes, 12 and six ones that are remarkably up- and downregulated, respectively, specifically in intense diapause were manually screened out in present study. To validate the expression of these genes throughout the pupal stage, the quantitative real-time PCR (qRT-PCR) was conducted, and the genes displaying different expression patterns with those of previous study were excluded. Then, the expressions of remaining genes were compared between diapause-destined and non-diapause-destined pupae to reveal their association with diapause using qRT-PCR and semiquantitative PCR. Finally, five genes, TTLL3B, Cyp6a9, MSTA, Fru, and UC2, and two genes, KSPI and LYZ1, were demonstrated to be positively and negatively associated with diapause, respectively. These findings provide a solid foundation for the further investigation of molecular mechanisms underlying B. minax pupal diapause.
Integrated Analysis of Clinical and Microbiome Risk Factors Associated with the Development of Oral Candidiasis during Cancer Chemotherapy
Authors: Diaz Patricia , Hong Bo-Young , Dupuy Amanda , Choquette Linda , Thompson Angela , Salner Andrew , Schauer Peter , Hegde Upendra , Burleson Joseph , Strausbaugh Linda , Peterson Douglas , Dongari-Bagtzoglou Anna
Published: June 2019

Oral candidiasis is a common side effect of cancer chemotherapy. To better understand predisposing factors, we followed forty-five subjects who received 5-fluorouracil- or doxorubicin-based treatment, during one chemotherapy cycle. Subjects were evaluated at baseline, prior to the first infusion, and at three additional visits within a two-week window. We assessed the demographic, medical and oral health parameters, neutrophil surveillance, and characterized the salivary bacteriome and mycobiome communities through amplicon high throughput sequencing. Twenty percent of all subjects developed oral candidiasis. Using multivariate statistics, we identified smoking, amount of dental plaque, low bacteriome and mycobiome alpha-diversity, and the proportions of specific bacterial and fungal taxa as baseline predictors of oral candidiasis development during the treatment cycle. All subjects who developed oral candidiasis had baseline microbiome communities dominated by Candida and enriched in aciduric bacteria. Longitudinally, oral candidiasis was associated with a decrease in salivary flow prior to lesion development, and occurred simultaneously or before oral mucositis. Candidiasis was also longitudinally associated with a decrease in peripheral neutrophils but increased the neutrophil killing capacity of Candida albicans. Oral candidiasis was not found to be associated with mycobiome structure shifts during the cycle but was the result of an increase in Candida load, with C. albicans and Candida dubliniensis being the most abundant species comprising the salivary mycobiome of the affected subjects. In conclusion, we identified a set of clinical and microbiome baseline factors associated with susceptibility to oral candidiasis, which might be useful tools in identifying at risk individuals, prior to chemotherapy.
N-Propargylation of Indolo-Triterpenoids and Their Application in Mannich Reaction
Authors: Khusnutdinova Elmira F. , Petrova Anastasiya V. , Bashirova Gulnaz M. , Kazakova Oxana B.
Published: June 2019

The introduction of the alkynyl moiety to the triterpenic core through a linkage to the indole nitrogen is described. The reaction of N-propargylindoles with N-methylpiperazine using Mannich reaction led to propargylaminoalkynyl-triterpenoids, whose structures were established by NMR spectroscopy.
Effect of Inhibitor on Adsorption Behavior and Mechanism of Micro-Zone Corrosion on Carbon Steel
Authors: Wang Fengjuan , Zhang Zhifeng , Wu Shengping , Jiang Jinyang , Chu Hongyan
Published: June 2019

A new type of inhibitor is studied in this paper. Inhibition efficiency and adsorption behavior of an inhibitor film on the steel surface is tested via the electrochemical method and theoretical calculation to establish the adsorption model. Test results confirm that inhibition efficiency is improved with the addition of an inhibitor, and the inhibitor film is formed firmly by comparing the characteristic peaks of S and N. Moreover, the micro-zone corrosion progress of Fe in 3.5% invasive NaCl-simulated seawater environment is studied. The results further show that corrosion is initiated under the zone without the inhibitor film, while it is prevented under the protection of the film. By the experiments, it is shown that inhibitor can be adsorbed on the surface of steel stably and has excellent protection performance for reinforced rebar, which can be widely used in concrete structure.
The Use of Cashew Nut Shell Liquid (CNSL) in PP/HIPS Blends: Morphological, Thermal, Mechanical and Rheological Properties
Authors: Araújo Mirna Nunes , Visconte Leila Lea Yuan , Barreto Daniel Weingart , Escócio Viviane Alves , Silva Ana Lucia Nazareth da , Sousa Ana Maria Furtado de , Pacheco Elen Beatriz Acordi Vasques
Published: June 2019

Polypropylene (PP) and high impact polystyrene (HIPS) are two polymers that are frequently found in disposable waste. Both of these polymers are restricted from being separated in several ways. An easier way to reuse them in new applications, without the need for separation, would require them to be less immiscible. In this work, cashew nut shell liquid (CNSL), a sub-product of the cashew agroindustry, was added as a third component to PP-HIPS mixtures and its effect as a compatibilizing agent was investigated. Morphological results showed that CNSL acted as an emulsifier by promoting reduction in the domains of the dispersive phase, HIPS, thus stabilizing the blends morphology. Differential scanning calorimetry (DSC) analysis suggests that CNSL is preferably incorporated in the HIPS phase. Its plasticizing effect leads to more flexible materials, but no significant effect could be detected on impact resistance or elongation at break.
Shear Performance Assessment of Sand-Coated GFRP Perforated Connectors Embedded in Concrete
Authors: Xiong Zhihua , Liu Yuqing , Zuo Yize , Xin Haohui
Published: June 2019

In order to evaluate the shear performance of sand-coated glass fiber-reinforced polymer (GFRP) perforated connectors (SCGPC) embedded in concrete, 8 pull-out tests were conducted. Finite element (FE) analysis considering GFRP failure and cohesion between GFRP and concrete of SCGPC were conducted for parametric analysis. Effects of surface treatment, hole’s radius, embedment length, and multi holes were examined. The test and theoretical analysis revealed that the strength of SCGPC is considerably larger than GFRP Perforated Connector (GPC). The stiffness of SCGPC is determined by the adhesion between concrete and GFRP. When GFRP plate’s thickness is less than the critical thickness, the embedment length plays a major role in the strength of SCGPC. When embedment length is less than the effective bond length, the shear strength of SCGPC is governed by both the adhesion and GPC’s shear capacity; otherwise, the strength of SCGPC is governed by the adhesion strength. Furthermore, an empirical equation was suggested to predict the shear strength of SCGPC. The equation involves the failure mechanism of both bond and GPC and deals the strength of SCGPC into two ranges according to the embedment length. Good agreement was achieved between the strength prediction by the suggested equation and the parametric analysis result.
A Statistical Damage Constitutive Model Based on the Weibull Distribution for Alkali-Resistant Glass Fiber Reinforced Concrete
Authors: Zhu Zhende , Zhang Cong , Meng Songsong , Shi Zhenyue , Tao Shanzhi , Zhu Duan
Published: June 2019

The addition of alkali-resistant glass fiber to concrete effectively suppresses the damage evolution such as microcrack initiation, expansion, and nucleation and inhibits the development and penetration of microcracks, which is very important for the long-term stability and safety of concrete structures. We conducted indoor flat tensile tests to determine the occurrence and development of cracks in alkali-resistant glass fiber reinforced concrete (AR-GFRC). The composite material theory and Krajcinovic vector damage theory were used to correct the quantitative expressions of the fiber discontinuity and the elastic modulus of the concrete. The Weibull distribution function was used and an equation describing the damage evolution of the AR-GFRC was derived. The constitutive equation was validated using numerical parameter calculations based on the elastic modulus, the fiber content, and a performance test of polypropylene fiber. The results showed that the tensile strength and peak strength of the specimen were highest at a concrete fiber content of 1%. The changes in the macroscopic stress–strain curve of the AR-GFRC were determined and characterized by the model. The results of this study provide theoretical support and reference data to ensure safety and reliability for practical concrete engineering.
The Preparation and Characterization of MnFe2O4-Decorated Expanded Graphite for Removal of Heavy Oils from Water
Authors: Tuan Nguyen Hoang Doan , Nguyen Hoang Tung , Nguyen Thuong Thi , Le Thi Ai Kha , Nguyen Thanh Duy , Phuong Bui Quynh Thi , Bach Long Giang
Published: June 2019

Recently, many methods have been developed to efficiently eliminate oil spills due to its long-term harmful effects on marine life and human health. Expanded graphite (EG) has been considered as an excellent platform to remove contaminated oil from aqueous solution through a facile adsorption route. As an innovative approach, the decoration of magnetic components, namely, MnFe2O4, into graphite layers was taken into account for facilitating phase separation under magnetic field which resulted into an easy collection of the used adsorbents in a large scale. The expanded graphite/manganese ferrite composites were prepared from Vietnamese graphite flakes via a two-stage process. Characterization was performed using Scanning Electron Microscope (SEM), Fourier-Transform Infrared Spectroscopy (FTIR), X-Ray Powder Diffraction (XRD), Vibrating Sample Magnetometer (VSM), Energy-Dispersive X-ray (EDS), and nitrogen adsorption/desorption analysis. The adsorption behavior of EG-MnFe2O4 for widespread used heavy oils, including diesel oil and crude oil, was investigated under the effects of adsorption conditions, i.e., contact time, loaded oil dosage, and salinity of mixing oil and water. The obtained results showed successful incorporation of MnFe2O4 into graphite sheets and no considerable change on the worm-like structure of EG. The results also showed that incorporated manganese ferrites enhanced the magnetism EG up to 16 emu/g, which made the recovery of used adsorbent conveniently. The EG-MnFe2O4 adsorbents exhibited the strong adsorption ability toward diesel oil (32.20 ± 0.46 g DO/g EG) and crude oil (33.07 ± 0.33 g CO/g EG). In brief, EG-MnFe2O4 material provides a potential and promising platform with high performance for oil spill removal.
Improving the Magnetic Properties of Non-Oriented Electrical Steels by Secondary Recrystallization Using Dynamic Heating Conditions
Authors: Petryshynets Ivan , Kováč František , Petrov Branislav , Falat Ladislav , Puchý Viktor
Published: June 2019

In the present work, we have used unconventional short-term secondary recrystallization heat treatment employing extraordinary high heating rate to develop coarse-grained microstructure with enhanced intensity of rotating cube texture {100}<011> in semi-finish vacuum degassed non-oriented electrical steels. The soft magnetic properties were improved through the increase of grains size with favourable cube crystallographic orientation. The appropriate final textural state of the treated experimental steels was achieved by strain-induced grain boundary migration mechanism, activated by gradient of accumulated stored deformation energy between neighbouring grains after the application of soft cold work, combined with steep temperature gradient during subsequent heat treatment under dynamic heating conditions. The materials in our experimentally prepared material states were mounted on the stator and rotor segments of electrical motors and examined for their efficiency in real operational conditions. Moreover, conventionally long-term heat treated materials, prepared in industrial conditions, were also tested for reference. The results show that the electrical motor containing the segments treated by our innovative approach, exhibits more than 1.2% higher efficiency, compared to the motor containing conventionally heat treated materials. The obtained efficiency enhancement can be directly related to the improved microstructural and textural characteristics of our unconventionally heat treated materials, specifically the homogenous coarse grained microstructure and the high intensity of cube and Goss crystallographic texture.
Effect of High Pressure on the Reducibility and Dispersion of the Active Phase of Fischer–Tropsch Catalysts
Authors: Yunes Simón , Vicente Miguel Ángel , Korili Sophia A. , Gil Antonio
Published: June 2019

The effect of high pressure on the reducibility and dispersion of oxides of Co and Fe supported on γ-Al2O3, SiO2, and TiO2 has been studied. The catalysts, having a nominal metal content of 10 wt.%, were prepared by incipient wetness impregnation of previously calcined supports. After drying at 60 °C for 6 h and calcination at 500 °C for 4 h, the catalysts were reduced by hydrogen at two pressures, 1 and 25 bar. The metal reduction was studied by temperature-programmed reduction up to 750 °C at the two pressures, and the metal dispersion was measured by CO chemisorption at 25 °C, obtaining values between 1% and 8%. The physicochemical characterization of these materials was completed by means of chemical analysis, X-ray diffraction, N2 adsorption-desorption at −196 °C and scanning electron microscopy. The high pressure lowered the reduction temperature of the metal oxides, improving their reducibility and dispersion. The metal reducibility increased from 42%, in the case of Fe/Al2O3 (1 bar), to 100%, in the case of Fe/TiO2 (25 bar).
Kabirimine, a New Cyclic Imine from an Okinawan Dinoflagellate
Authors: Hermawan Idam , Higa Mikako , Hutabarat Philipus Uli Basa , Fujiwara Takeshi , Akiyama Kiyotaka , Kanamoto Akihiko , Haruyama Takahiro , Kobayashi Nobuyuki , Higashi Masahiro , Suda Shoichiro , Tanaka Junichi
Published: June 2019

On our quest for new bioactive molecules from marine sources, two cyclic imines (1, 2) were isolated from a dinoflagellate extract, inhibiting the growth of the respiratory syncytial virus (RSV). Compound 1 was identified as a known molecule portimine, while 2 was elucidated to be a new cyclic imine, named kabirimine. The absolute stereochemistry of 1 was determined by crystallographic work and chiral derivatization, whereas the structure of 2 was elucidated by means of spectroscopic analysis and computational study on all the possible isomers. Compound 1 showed potent cytotoxicity (CC50 < 0.097 µM) against HEp2 cells, while 2 exhibited moderate antiviral activity against RSV with IC50 = 4.20 µM (95% CI 3.31–5.33).
Thermodynamic Modelling of Iron Ore Sintering Reactions
Authors: Chen Chunlin , Lu Liming , Jiao Kexin
Published: June 2019

Silico-ferrite of calcium and aluminum (SFCA) is one of the most commonly-produced phases in fluxed iron-ore sintering, and has long been regarded as an important bonding phase in industrial sinters. It is thus considered to have a significant effect on sinter quality. In this study, a solid solution model and database has been developed for the SFCA phase, and has been incorporated into the thermodynamic software, Multi-Phase Equilibrium (MPE). MPE calculations were compared with the in situ X-ray powder diffraction (XRD) observations of the formation of SFCA phase during sintering. The effects of the raw material composition, temperature and the oxygen partial pressure on the formation of mineral phases in the sinter, as well as the viscosity of the melt formed during sintering under equilibrium conditions, were modelled using MPE. The results show that the formation of SFCA phase can be promoted by increasing oxygen partial pressure and basicity of the raw material. Increases of Al2O3 and MgO content have no significant effect on the SFCA formation under equilibrium condition. The increase of oxygen partial pressure (10−3 atm or above) and basicity also leads to a decrease in melt viscosity, which enhances the fluidity of the melt, and hence, the assimilation of the sinter. However, increases of Al2O3 and MgO result in the increase of melt viscosity.
Current Aspects of siRNA Bioconjugate for In Vitro and In Vivo Delivery
Authors: Tai Wanyi
Published: June 2019

Studies on siRNA delivery have seen intense growth in the past decades since siRNA has emerged as a new class of gene therapeutics for the treatment of various diseases. siRNA bioconjugate, as one of the major delivery strategies, offers the potential to enhance and broaden pharmacological properties of siRNA, while minimizing the heterogeneity and stability-correlated toxicology. This review summarizes the recent developments of siRNA bioconjugate, including the conjugation with antibody, peptide, aptamer, small chemical, lipidoid, cell-penetrating peptide polymer, and nanoparticle. These siRNA bioconjugate, either administrated alone or formulated with other agents, could significantly improve pharmacokinetic behavior, enhance the biological half-life, and increase the targetability while maintaining sufficient gene silencing activity, with a concomitant improvement of the therapeutic outcomes and diminishment of adverse effects. This review emphasizes the delivery application of these siRNA bioconjugates, especially the conjugation strategy that control the integrity, stability and release of siRNA bioconjugates. The limitations conferred by these conjugation strategies have also been covered.
Antiplatelet Activity of Acylphloroglucinol Derivatives Isolated from Dryopteris crassirhizoma
Authors: Yim Nam-Hui , Lee Jung-Jin , Lee BoHyoung , Li Wei , Ma Jin Yeul
Published: June 2019

Platelets are an important component of the initial response to vascular endothelial injury; however, platelet dysfunction induces the acute clinical symptoms of thrombotic disorders, which trigger severe cardiovascular diseases such as myocardial infarction, ischemia, and stroke. In this study, we investigated the Dryopteris crassirhizoma’s antiplatelet activity. A water extract of D. crassirhizoma (WDC) was partitioned into dichloromethane (DCM), ethyl acetate, n-butyl alcohol, and water. Among these four fractions, the DCM fraction potently inhibited the collagen-stimulated platelet aggregation in a concentration-dependent manner. From this fraction, five different acylphloroglucinol compounds and one flavonoid were isolated by activity-guided column chromatography. They were identified by comparing their mass, 1H-, and 13C-NMR spectral data with those reported in the literature. Quantifying the six compounds in WDC and its DCM fraction by high-performance liquid chromatography (HPLC) revealed that butyryl-3-methylphloroglucinol (compound 4) was the most abundant in these samples. Additionally, butyryl-3-methylphloroglucinol showed the strongest inhibitory activity in the collagen- and arachidonic acid (AA)-induced platelet aggregation, with inhibition ratios of 92.36% and 89.51% in the collagen and AA-induced platelet aggregation, respectively, without cytotoxicity. On the active concentrations, butyryl-3-methylphloroglucinol significantly suppressed the convulxin-induced platelet activation. Regarding the structure–activity relationships for the five acylphloroglucinol compounds, our results demonstrated that the functional butanonyl, methoxy, and hydroxy groups in butyryl-3-methylphloroglucinol play important roles in antiplatelet activity. The findings indicate that acylphloroglucinols, including butyryl-3-methylphloroglucinol from D. crassirhizom, possess an antiplatelet activity, supporting the use of this species for antiplatelet remedies.
Putative Roles of Plant-Derived Tannins in Neurodegenerative and Neuropsychiatry Disorders: An Updated Review
Authors: Hussain Ghulam , Huang Jia , Rasul Azhar , Anwar Haseeb , Imran Ali , Maqbool Javeria , Razzaq Aroona , Aziz Nimra , Makhdoom Ehtisham ul Haq , Konuk Muhsin , Sun Tao
Published: June 2019

Neurodegenerative and neuropsychiatric diseases are characterized by the structural and functional abnormalities of neurons in certain regions of the brain. These abnormalities, which can result in progressive neuronal degeneration and functional disability, are incurable to date. Although comprehensive efforts have been made to figure out effective therapies against these diseases, partial success has been achieved and complete functional recovery is still not a reality. At present, plants and plant-derived compounds are getting more attention because of a plethora of pharmacological properties, and they are proving to be a better and safer target as therapeutic interventions. This review aims to highlight the roles of tannins, ‘the polyphenol phytochemicals’, in tackling neurodegenerative diseases including Alzheimer’s and Parkinson’s diseases as well as neuropsychiatric disorders like depression. Among the multifarious pharmacological properties of tannins, anti-oxidative, anti-inflammatory, and anti-cholinesterase activities are emphasized more in terms of neuroprotection. The current review also throws light on mechanistic pathways by which various classes of tannins execute neuroprotective effects. Despite their beneficial properties, some harmful effects of tannins have also been elaborated.
Porous Silicon-Based Aptasensors: The Next Generation of Label-Free Devices for Health Monitoring
Authors: Terracciano Monica , Rea Ilaria , Borbone Nicola , Moretta Rosalba , Oliviero Giorgia , Piccialli Gennaro , De Stefano Luca
Published: June 2019

Aptamers are artificial nucleic acid ligands identified and obtained from combinatorial libraries of synthetic nucleic acids through the in vitro process SELEX (systematic evolution of ligands by exponential enrichment). Aptamers are able to bind an ample range of non-nucleic acid targets with great specificity and affinity. Devices based on aptamers as bio-recognition elements open up a new generation of biosensors called aptasensors. This review focuses on some recent achievements in the design of advanced label-free optical aptasensors using porous silicon (PSi) as a transducer surface for the detection of pathogenic microorganisms and diagnostic molecules with high sensitivity, reliability and low limit of detection (LoD).
Serum N-Glycosylation in Parkinson’s Disease: A Novel Approach for Potential Alterations
Authors: Váradi Csaba , Nehéz Károly , Hornyák Olivér , Viskolcz Béla , Bones Jonathan
Published: June 2019

In this study, we present the application of a novel capillary electrophoresis (CE) method in combination with label-free quantitation and support vector machine-based feature selection (support vector machine-estimated recursive feature elimination or SVM-RFE) to identify potential glycan alterations in Parkinson’s disease. Specific focus was placed on the use of neutral coated capillaries, by a dynamic capillary coating strategy, to ensure stable and repeatable separations without the need of non-mass spectrometry (MS) friendly additives within the separation electrolyte. The developed online dynamic coating strategy was applied to identify serum N-glycosylation by CE-MS/MS in combination with exoglycosidase sequencing. The annotated structures were quantified in 15 controls and 15 Parkinson’s disease patients by label-free quantitation. Lower sialylation and increased fucosylation were found in Parkinson’s disease patients on tri-antennary glycans with 2 and 3 terminal sialic acids. The set of potential glycan alterations was narrowed by a recursive feature elimination algorithm resulting in the efficient classification of male patients.