bluuebunny/crossref_metadata_2025 huggingface dataset:
Catalytic Hydrogenation Property of Methyl Benzoate to Benzyl Aldehyde over Manganese-Based Catalysts with Appropriate Oxygen Vacancies
Authors: Gao Pengxiang , Liu Xiaoran , Mu Xindong , Zhang Yan
Published: December 2023

The synthesis of benzaldehyde, a compound widely utilized in food, medicine, and cosmetics, was achieved through a one-step catalytic hydrogenation using the cost-effective raw material, methyl benzoate. This process aligns with the principles of atom economy and green production. Despite the development of numerous high-performance catalysts by scholars, the challenge remains in achieving lower reaction temperatures, ideally below 400 °C. In this study, a series of MnOx/γ-Al2O3 catalysts were meticulously prepared using the precipitation-impregnation method. These catalysts featured supports calcined at various temperatures and distinct manganese active components. Characterization techniques such as X-ray diffraction (XRD), N2 physical adsorption, Fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), H2 temperature programmed reduction (H2-TPR), and NH3 temperature-programmed desorption (NH3-TPD) were employed to analyze the structure and surface properties of the catalysts. Notably, the optimized reaction temperature was found to be 360 °C. The catalyst exhibited the most favorable performance when the calcination temperature of the support was 500 °C and the Mn/Al molar ratio reached 0.18. Under these conditions, the catalyst demonstrated the most suitable oxygen vacancy concentration, yielding impressive results: a conversion rate of 87.90% and a benzaldehyde selectivity of 86.1%. These achievements were attained at 360 °C, atmospheric pressure, a hydrogen to methyl benzoate molar ratio of 40:1, and a Gas Hourly Space Velocity (GHSV) of 800 h−1. This research underscores the potential for optimizing catalysts to enhance the efficiency and sustainability of benzaldehyde synthesis.
CREB1 Facilitates GABAergic Neural Differentiation of Human Mesenchymal Stem Cells through BRN2 for Pain Alleviation and Locomotion Recovery after Spinal Cord Injury
Authors: Kao Yanbing , Zhu Hanming , Yang Yu , Shen Wenyuan , Song Wei , Zhang Renjie , Liu Yanchun , Liu Haoyun , Kong Xiaohong
Published: December 2023

The transplantation of GABAergic neuron cells has been reported to alleviate nerve pain and improve motor function after spinal cord injury (SCI). However, human mesenchymal stem cell (hMSC) differentiation into GABAergic neuron cells in a sufficient quantity remains to be accomplished. From a database screening, cAMP-responsive element-binding protein 1 (CREB1) was chosen as a potential modulator due to its critical role in the protein–protein interaction of genes related to GABAergic neural differentiation. Here, CREB1 was overexpressed in transfected hMSCs, where CREB1 could induce differentiation into GABAergic neuron cells with an upregulation of Map2 and GAD1 by 2- and 3.4-fold, respectively. Additionally, GABAergic neural differentiation was enhanced, while Notch signaling was inhibited, and BRN2 transcriptional activation played an important role in neuronal maturation. Moreover, transfected hMSCs injected into immunocompromised mice caused by CsA exhibited the neuronal markers Tuj1 and Map2 via the intraspinal route, suggesting an improvement in survival and neural differentiation. Significantly, improvement in both BMS scores (6.2 ± 1.30 vs. 4 ± 0) and thermal hyperalgesia latency (7.74 ± 2.36 s vs. 4.52 ± 0.39 s) was seen compared with the SCI naïve treatment at 4 weeks post-transplantation. Our study demonstrates that CREB1 is crucial in generating induced GABAergic neuron cells (iGNs) originating from hMSCs. Transplanting iGNs to injured spinal cord provides a promising strategy for alleviating neuropathic pain and locomotion recovery after SCI.
Dysregulation of Lymphatic Endothelial VEGFR3 Signaling in Disease
Authors: Kuonqui Kevin , Campbell Adana-Christine , Sarker Ananta , Roberts Arielle , Pollack Bracha L. , Park Hyeung Ju , Shin Jinyeon , Brown Stav , Mehrara Babak J. , Kataru Raghu P.
Published: December 2023

Vascular endothelial growth factor (VEGF) receptor 3 (VEGFR3), a receptor tyrosine kinase encoded by the FLT4 gene, plays a significant role in the morphogenesis and maintenance of lymphatic vessels. Under both normal and pathologic conditions, VEGF-C and VEGF-D bind VEGFR3 on the surface of lymphatic endothelial cells (LECs) and induce lymphatic proliferation, migration, and survival by activating intracellular PI3K-Akt and MAPK-ERK signaling pathways. Impaired lymphatic function and VEGFR3 signaling has been linked with a myriad of commonly encountered clinical conditions. This review provides a brief overview of intracellular VEGFR3 signaling in LECs and explores examples of dysregulated VEGFR3 signaling in various disease states, including (1) lymphedema, (2) tumor growth and metastasis, (3) obesity and metabolic syndrome, (4) organ transplant rejection, and (5) autoimmune disorders. A more complete understanding of the molecular mechanisms underlying the lymphatic pathology of each disease will allow for the development of novel strategies to treat these chronic and often debilitating illnesses.
Coupling Effect of Disconnected Pores and Grain Morphology on the Corrosion Tolerance of Laser-Clad 316L Coating
Authors: Zhang San-Qi , Dong Hui , Han Yan , Xu Long , Feng Yu-Kun , Li Peng-Yu
Published: December 2023

The corrosion resistance of 316L cladding layers was addressed via the electrochemical test, to illustrate the coupling effect of the disconnected pores and grain morphology on the corrosion tolerance of 316L cladding layers. Scanning electron microscopy (SEM), X-ray diffraction (XRD), and electrochemical testing were employed to characterize the microstructure, elemental distribution, phase composition, and corrosion resistance of the cladding layers. The results indicate that the disconnected porosity in the surface of the cladding layer decreased from 0.79% to 0.48% and the grain morphology underwent a transformation from equiaxed crystals to columnar and lath crystals, with the increasing scanning speed. The primary phase in the cladding layer was γ-Fe. Under the dual effect of a low disconnected porosity and grain morphology, the corrosion potential of the cladding layer became more electropositive from −568 mVSCE to −307 mVSCE, and the corrosion current density reduced from 4.664 μA∙cm−2 to 1.645 μA∙cm−2. The pitting potential improved from 0.005 VSCE to 0.575 VSCE as the scanning speed increased. Thus, the non-connected pores in the 316L cladding layer also affected the corrosion resistance, especially the pitting resistance. The corrosion resistance of the cladding layer can be significantly enhanced via the control of the disconnected pores and grain morphology.
Characterization of the Cracking Resistance Gradient of Bitumen Emulsion-Based Cold In-Place Recycling Mixtures over Curing by Semi-Circular Bending Test
Authors: Zhao Zili , Jiang Jiwang , Ni Fujian
Published: December 2023

To better reveal the performance development of bitumen emulsion-based cold in-place recycling (BE-CIR) mixture over curing, a semi-sealed laboratory curing method was proposed in this research to simulate the in situ moisture evaporation process and cracking resistance of the BE-CIR specimen at different depths during a curing time of 28 days, which was also investigated by the semi-circular bending (SCB) test. The influencing factors of cement content (1.5% to 2.5%), initial moisture content (3.5% to 4.5%), curing temperature (25 °C to 45 °C) and relative humidity were investigated, and the significance of different factors affecting the performance development was also analyzed. The results indicate significant variations in cracking performance parameters at different depths, with the top part exhibiting notably higher tensile strength and fracture energy compared to the bottom part, and a gradient index (GI) is proposed to describe the difference. Cement content affected early tensile strength and fracture energy, while the initial moisture content affected the development rate of the performance. The influence of curing temperature was extensive, and as the temperature increased beyond 40 °C, the strength of the effect decreased. High humidity during the early stage of curing inhibited the strength formation and development of fracture energy. The performance development of the BE-CIR mixture is more significantly influenced by the moisture migration process, which is governed by curing temperature and relative humidity, as opposed to the cement content and initial moisture content.
Study on the Microstructure and Properties of FeCoNiCrAl High-Entropy Alloy Coating Prepared by Laser Cladding-Remelting
Authors: Lv Tianyi , Zou Wenkai , He Jiaqi , Ju Xiang , Zheng Chuanbo
Published: December 2023

Laser remelting technology effectively repairs defects such as pores and cracks in the coating. To investigate the impact of laser remelting on high-entropy alloy coatings, this study used Q235 steel as the substrate and employed laser cladding technology to prepare FeCoNiCrAl high-entropy alloy coatings, followed by laser remelting treatment. The phase composition and microstructure of the coatings were extensively characterized using equipment such as optical microscopy, X-ray diffraction (XRD), and scanning electron microscopy (SEM). Additionally, the wear resistance and corrosion resistance of the coatings were tested using a multifunctional material surface performance tester, an electrochemical workstation, and SVET (Scanning Vibrating Electrode Technique). The results indicate that following laser remelting treatment, the atomic proportion of Fe elements on the coating surface decreased from 33.21% to 26.03%, while the atomic proportion of Al elements increased from 12.56% to 20.31%. The phase composition of the coating underwent a marked transformation, shifting from a structure composed of FCC, A2, and B2 phases to a singular BCC structure characterized by the presence of A2 and B2 phases. Concurrently, the grain morphology on the coating surface transitioned from elongated plate-like grains to equiaxed grains. Laser remelting enhanced the wear resistance of the coating. Laser remelting had no significant impact on the corrosion resistance of the non-cracked regions of the coating.
Effects of the Number of Layers and Thickness Ratio on the Impact Fracture Behavior of AA6061/AA7075 Laminated Metal Composites
Authors: Chen Zejun , Lu Guangming , Zhou Dayu , Huang Guangjie , Cao Yu
Published: December 2023

The initial thickness ratio and number of layers of dissimilar metal components greatly influence the impact performance of laminated metal composites. In this paper, positive and lateral impact tests of 5-layer composite sheets with thickness ratios of 3:1, 1.35:1, and 1:2 and 80-layer composite sheets prepared by ARB (accumulative roll bonding) were conducted to study the influences of the thickness ratio and layer number on the impact fracture behavior of composite sheets. The results showed that the higher the proportion of AA7075, the higher the bending strength of the AA6061/AA7075 laminated composite sheet; compared with the 5-layer composite sheet, the side impact performance of the 80-layer composite sheet is obviously improved, and its side impact strength, energy absorbed in the crack initiation stage, and crack propagation stage are better than those of the 5-layer composite sheet. In addition, the toughening mechanism of the 80-layer composite sheet is mainly that the increase in the number of layers makes the cracks deflect more frequently. Under the rapid impact load, the impact energy absorbed by the sample increases with the increase in the number of layers.
Mechanisms and Potential Clinical Implications of Oral Microbiome in Oral Squamous Cell Carcinoma
Authors: Wang Jingyi , Gao Bo
Published: December 2023

Microorganisms in the oral cavity are abundant in the human body. At present, more than 700 species of oral microorganisms have been identified. Recently, a lot of literature has indicated that the oral microbiota plays an important role in the occurrence, development, and prognosis of oral squamous cell carcinoma (OSCC) through various mechanisms. And researchers are now trying to utilize oral microbiota in cancer diagnosis and treatment. However, few articles systematically summarize the effects of oral microbes in the diagnosis, treatment, and disease outcomes of oral cancer. Herein, we made a summary of the microbial changes at cancerous sites and placed more emphasis on the mechanisms by which the oral microbiome promotes cancerization. Moreover, we aimed to find out the clinical value of the oral microbiome in OSCC.
Unknown Is Not Chosen: University Student Voices on Group Formation for Collaborative Writing
Authors: Putzeys Karen , Van Keer Hilde , De Wever Bram
Published: December 2023

University students are frequently required to collaborate, often in the form of collaborative writing tasks. The process as well as the outcomes of the collaboration depend on choices made during the group formation phase. Studies on why students select partners for collaborative writing tasks are, however, lacking. Therefore, the present study aims to gain insights into (1) university students’ preferences with regard to teacher-assigned and self-selected group formation, (2) which motives they take into account when self-selecting a partner, and (3) the degree to which students select a partner similar to themselves. Sixteen dyads collaboratively wrote a research paper. Prior to the collaboration, 30 students individually completed questionnaires and 28 students were individually interviewed. The findings show that most students have mixed opinions regarding teacher-assigned or self-selected group formation (n = 18), while the others bar one prefer to self-select a partner (n = 9). Students’ main motive for self-selecting is familiarity, and, more in particular, prior collaboration experience with a specific partner. Other motives include friendship, ability, convenience, and attitude. Furthermore, students tend to select a partner with a similar attitude, ability, task approach, and perspective towards the content of the task. Predictability seems to be the most important driver for self-selection.
Enhancing LOCA Breach Size Diagnosis with Fundamental Deep Learning Models and Optimized Dataset Construction
Authors: Xiao Xingyu , Qi Ben , Liang Jingang , Tong Jiejuan , Deng Qing , Chen Peng
Published: December 2023

In nuclear power plants, the loss-of-coolant accident (LOCA) stands out as the most prevalent and consequential incident. Accurate breach size diagnosis is crucial for the mitigation of LOCAs, and identifying the cause of an accident can prevent catastrophic consequences. Traditional methods mostly focus on combining model algorithms and utilize intricate composite model neural network architectures. However, it is crucial to investigate whether greater complexity necessarily leads to better performance. In addition, the consideration of the impact of dataset construction and data preprocessing on model performance is also needed for model building. This paper proposes a framework named DeepLOCA-Lattice to experiment with different preprocessing approaches to fundamental deep learning models for a comprehensive analysis of the diagnosis of LOCA breach size. The DeepLOCA-Lattice involves data preprocessing via the lattice algorithm and equal-interval partitioning and deep-learning-based models, including the multi-layer perceptron (MLP), recurrent neural networks (RNNs), convolutional neural networks (CNNs), and the transformer model in LOCA breach size diagnosis. After conducting rigorous ablation experiments, we have discovered that even rudimentary foundational models can achieve accuracy rates that exceed 90%. This is a significant improvement when compared to the previous models, which yield an accuracy rate of lower than 50%. The results interestingly demonstrate the superior performance and efficacy of the fundamental deep learning model, with an effective dataset construction approach. It elucidates the presence of a complex interplay among diagnostic scales, sliding window size, and sliding stride. Furthermore, our investigation reveals that the model attains its highest accuracy within the discussed range when utilizing a smaller sliding stride size and a longer sliding window length. This study could furnish valuable insights for constructing models for LOCA breach size estimation.
Design, Simulation, and Fabrication of a 500 kV Ultrawideband Coaxial Matched Load and Its Connectors for Fast Transient Pulse Measurement Systems
Authors: Khan Mohammad Saif , Agazar Mohamed , Le Bihan Yann
Published: December 2023

In the past few decades, Pulsed Power (PP) has been one of the fastest growing technologies, with more and more systems frequently emerging in domains such as civil, medical and military. These systems are based on high-voltage pulses, up to several hundreds of kilovolts, with temporal parameters ranging from microsecond levels to sub-nanosecond levels. One of the biggest challenges in this technology is the accurate and precise measurement of the generated PP. The PP measurement systems must possess high-voltage and wideband properties simultaneously, which is often conflicting. The central elements of a PP measurement system are a voltage divider and a termination load. The work presented in this article is dedicated to the second element of the PP measurement system. This paper describes the development of a 50 Ω coaxial termination load and its connectors for a high power ultrawideband (UWB) pulse measurement systems. The principle roles of these devices are to serve as a dummy matched load for the former and to facilitate the connections between different components of the pulse measurement system for the latter. These devices are designed to withstand pulse voltage amplitudes at least up to 500 kV with temporal parameters, such as rise time and pulse duration, varying from nanosecond to sub-nanosecond ranges. The main challenge in the development of a high-voltage UWB termination load is the tradeoff between the high-voltage and wideband characteristics, both of them requiring opposite dimensional aspects for the load device. This challenge is overcame by the special exponential geometry of the load device. The design employs a 30 cm long low-inductance tubular ceramic 50 Ω resistor, enclosed in a critically dimensioned shielding conductor of an exponential inner profile. This shrinking coaxial structure makes it possible to maintain a good level of matching all along the 50 Ω load. The results obtained through 3D electromagnetic modeling and vector network analyzer measurements show good agreement and confirm the reflection coefficient below −27 dB up to at least 2.5 GHz for the load device. Moreover, calculations demonstrate that the load device is very well adapted for nanosecond and sub-nanosecond pulses with voltage peaks as high as 500 kV. These results demonstrate the high-voltage and UWB properties of the developed load device and prove the utilization of this device in the measurement systems for the accurate and precise measurements of the PP.
Reuse of Oil Wells in Geothermal District Heating Networks: A Sustainable Opportunity for Cities of the Future
Authors: Alimonti Claudio , Vitali Fabio , Scrocca Davide
Published: December 2023

Climate change and the energy crisis forced industrialized countries to contain CO2 emissions and use indigenous renewable energy sources. Geothermal energy undoubtedly has great potential, particularly thermal energy, given that 48% of the final energy consumption in the EU20 countries in 2021 was related to heating and cooling systems. The present study verifies and compares the feasibility of realizing district heating systems in two different contexts: (i) depleted hydrocarbon fields with the repurposing of existing hydrocarbon wells into geothermal wells and (ii) areas with documented geothermal resources. The two selected case studies are located, respectively, near Romentino (Northern Italy, province of Novara) and Tuscania (Central Italy, province of Viterbo). Following an assessment of the geothermal resources in the two selected case studies, specific methodological tools have been developed to evaluate the energy demand in the municipalities and determine the projects’ economics. Both case studies show positive economic indices assuming heat tariffs aligned with the values recorded in the 2020–2021 period. However, our results show how reusing hydrocarbon wells in geothermal wells constitutes an excellent opportunity to access geothermal resources, significantly reducing the necessary investment and the mining risk and strongly improving the economics of the projects.
Recursive Neural Network as a Multiple Input–Multiple Output Speed Controller for Electrical Drive of Three-Mass System
Authors: Zawirski Krzysztof , Brock Stefan , Nowopolski Krzysztof
Published: December 2023

Electrical drive systems are commonly applied for the mechanisms of precise movement, where having a high-quality position and high-quality speed control is especially valuable. Very often, the mechanical part of these systems reveals resonant properties that are related to the limited stiffness of the interconnection between subsequent parts of the mechanism. In most cases, this sort of system may be described as a model of several linked masses. If only the structure of the mechanical part is known and the corresponding parameters are constant and identified, the demanded control quality may be obtained using a properly tuned ADRC or PID controller equipped with appropriate anti-resonance filtration. However, if the parameters of the mechanical part are variant, adaptive control may be considered as a solution. In this paper, artificial neural network (ANN) is considered to be a speed controller and its training method assures adaptation to the unknown mechanical parameters. The paper is particularly focused on a three-mass system, which possesses, due to its structure, two resonant frequencies. The unique property of the analyzed system is the application of drive units at both ends of the system, so that the controller has the ability to influence the resonant system from both sides. The coordination of the drive unit is performed by the aforementioned ANN, from which two outputs affect the drive units independently. The derivation of the mathematical model is followed by its implementation in a computer simulation and finally the evaluation in a dedicated laboratory setup, the construction of which is also presented in the paper.
Universal Input Single-Stage High-Power-Factor LED Driver with Active Low-Frequency Current Ripple Suppressed
Authors: Hwu Kuo-Ing , Shieh Jenn-Jong , Lin Chien-Ting
Published: December 2023

In this paper, a light-emitting diode (LED) driver with a high power factor (PF) and low-frequency current ripple suppression over a wide input range is presented, and a flyback converter is designed to operate in the discontinuous conduction mode (DCM), with a digital controller used to keep the duty cycle constant for half of the utility cycle under a fixed load and input voltage. This method ensures that the input current is in phase with the universal input voltage, thus achieving a high power factor without utilizing feedforward control. Furthermore, on the secondary side, the time of the zero point of the utility voltage can be attained so that the duty cycle can be updated at this time. In addition, a simple auxiliary circuit is connected parallel to the output side to absorb the excess output current of the flyback converter or to release the current to the load to make up for the shortage of the output current of the flyback converter so that the low-frequency ripple of the output current can be inhibited. There are only two current-detecting resistors used in this study: one is the output current-sensing resistor of the flyback converter, and the other is the output current-sensing resistor of the auxiliary circuit.
Vegetation History Characteristics of Planted Forests in Japan: Analysis of the 1960 World Census of Agriculture and Forestry
Authors: Ueno Ryudai , Iwano Junna , Fujiwara Takahiro , Sato Noriko
Published: December 2023

This study aimed to clarify the vegetation history of planted forests that are still in use today by comparing the types of land afforested in 1960 with the post-war afforestation period in Japan’s Hokkaido, Kyushu, and Kinki regions. It was during that time that the majority of the planted forest in these regions was established. We calculated the afforestation ratios (afforested area of each type of land/total afforested area) for the smallest survey unit, the 1950 administrative sections, using data from the 1960 World Agriculture and Forestry Census on the afforested areas of various types of land (i.e., deforested areas resulting from natural forests, deforested areas resulting from planted forests, and areas other than forests). The afforestation ratios were then sorted into ten categories for each municipality in the three regions and mapped using GIS. The analysis results showed that the vegetation history in Kyushu, even within the same municipality, was frequently mixed in 1960; in Hokkaido, the afforestation ratio for deforested areas resulting from natural forests was extremely high, while the afforestation ratio for deforested areas resulting from planted forests was low. In contrast, the Kinki region has a low afforestation ratio for non-forest regions. This study provides useful information for ecosystem management.
Carbon Sequestration in the Aboveground Living Biomass of Windbreaks—Climate Change Mitigation by Means of Agroforestry in Hungary
Authors: Király Éva , Keserű Zsolt , Molnár Tamás , Szabó Orsolya , Borovics Attila
Published: December 2023

The land use sector is a crucial pillar in achieving the EU climate goals set for 2050. A significant part of the climate change mitigation potential of the land use sector is inherent to agroforestry. Windbreaks are important agroforestry elements of Hungarian agricultural landscapes. The new and improved agroforestry subsidy system may positively affect the extension of windbreaks in Hungary, making it relevant to assess their carbon sequestration potential. In our study, we examined the carbon sequestration of windbreaks at the country level and in two sample areas of 24,000 hectares based on National Forestry Database volume stock data, as well as information collected from the Hungarian Forest Cover Map using orthophoto interpretation. We estimated the total annual carbon sequestration realized in the aboveground biomass pool of Hungarian windbreaks to be −33.1 ktCO2/year, which is 0.67% of the total annual carbon sequestration of the aboveground biomass pool of all Hungarian forests, as reported by the Hungarian Greenhouse Gas Inventory. On the other hand, according to our estimate, the weighted mean annual carbon sequestration in the aboveground biomass of windbreaks was −2.4 tCO2/ha/year in the 2010–2020 period. This value is very close to the average mean annual carbon sequestration per hectare value of all forests, as reported by the Hungarian Greenhouse Gas Inventory. This means that planting a given area of windbreaks in between agricultural fields can have similar climate change mitigation effects as planting forests in the same given area.
Seasonal Dynamics in Mycorrhizal Colonization and Fine Root Features of the White Poplar (Populus alba L.) in Natural Temperate Riverside Forests with Two Contrasting Soils
Authors: Frymark-Szymkowiak Anna , Kulczyk-Skrzeszewska Magdalena , Tyburska-Woś Jolanta
Published: December 2023

Fine roots are the most dynamic and physiologically active components of belowground tree organs. However, much remains unknown regarding the changes in fine root morphological characteristics during mycorrhizal colonization, especially in natural sites. The aim of this study was to analyze seasonal heterogeneity in fine roots and the mycorrhizal colonization of mature white poplar (Populus alba L.) trees under different soil conditions. Two floodplain forests were selected in Central Europe (Poland), which differed in soil moisture and structure. Fine roots were sampled during one growing season from the upper soil layer. Poplars were characterized by dual mycorrhizal colonization on one root system. It was, therefore, possible to investigate the contribution of two mycorrhizal types (arbuscular mycorrhiza—AM; and ectomycorrhiza—ECM) in response to different habitat conditions. The season was shown to be significant for all fine root features, as well as the degree of mycorrhizal colonization. Roots were better adapted to a drier habitat with a greater proportion of sand, mainly due to a reduction in the fine root diameter (FRD), while other root characteristics did not differ significantly. The degree of mycorrhizal colonization (RLC) and the proportion of arbuscular mycorrhizal structures (AM) were significantly and negatively correlated with the soil water content. A mutual competition between arbuscular mycorrhizas and ectomycorrhizas for poplar roots was also observed, particularly with respect to the season, site, and soil moisture. Changing environmental conditions (especially soil moisture) contribute not only to the morphological and functional changes of fine roots but also to changes in the proportion of arbuscular mycorrhiza and ectomycorrhiza. Understanding the mechanisms of adaptation of tree roots to changing environmental conditions is especially important in the context of climate change.
Carpathian Forests: Past and Recent Developments
Authors: Kholiavchuk Dariia , Gurgiser Wolfgang , Mayr Stefan
Published: December 2023

Forests of the Carpathians are of increasing research interest, as they cover a large area (>9 Mha) within European forests and are influenced by diverse environmental conditions and contrasting historical developments. We reviewed 251 papers dealing with Carpathian forests, their history, and future perspectives. Over 70% of articles and reviews appeared in the last ten years, and 80% refer to the Western and Eastern Carpathians, while the Serbian Carpathians remain a gap in this research field. Forest expansion and species changes have occurred since Holocene deglaciation, influenced by timber use, settlements, cropland development, and, since the Bronze Age, pasture activities. At higher elevations, early conifer successors have been increasingly replaced by Norway spruce (Picea abies), silver fir (Abies alba), European beech (Fagus sylvatica), and hornbeam (Carpinus betulus), while oaks have been present in the Carpathian foothills throughout the whole of history. In the 19th and 20th centuries, Norway spruce afforestation was favored, and timber use peaked. Recent transitions from agriculture to forest land use have led to a further increase in forest cover (+1 to +14% in different countries), though past forest management practices and recent environmental changes have impaired forest vitality in many regions; climate warming already causes shifts in treelines and species distributions, and it triggers pest outbreaks and diseases and affects tree–water relations. The risk of forest damage is the highest in monodominant Norway spruce forests, which often experience dieback after cascade disturbances. European beech forests are more resilient unless affected by summer droughts. In the future, increasing dominance of broadleaves within Carpathian forests and forest management based on a mix of intensive management and ecological silviculture are expected. Maintenance and promotion of silver fir and mixed European beech forests should be encouraged with respect to forest stability, biodiversity, and economic sustainability. As supported by the Carpathian Convention and related institutions and initiatives, connectivity, management, and stakeholder cooperation across administrative borders will be crucial for the future adaptive potential of Carpathian forests.
Using ERIC to Assess Implementation Science in Drowning Prevention Interventions in High-Income Countries: A Systematic Review
Authors: Della Bona Malena , Crawford Gemma , Royce Brooklyn , Jancey Jonine , Leavy Justine E.
Published: December 2023

This systematic review identifies and describes the use of the Expert Recommendation for Implementing Change (ERIC) concepts and strategies using public health approaches to drowning prevention interventions as a case study. International calls for action have identified the need to better understand the implementation of drowning prevention interventions so that intervention design and implementation is improved. In high-income countries (HICs), interventions are sophisticated but still little is known or written about their implementation. The review was registered on PROSPERO (number CRD42022347789) and followed the PRISMA guidelines. Eight databases were searched. Articles were assessed using the Public Health Ontario Meta-tool for quality appraisal of public health evidence. Forty-nine articles were included. Where ERIC strategies were reported, the focus was on evaluative and iterative strategies, developing partnerships and engaging the target group. The review identified few articles that discussed intervention development and implementation sufficiently for strategies to be replicated. Findings will inform further research into the use and measurement of implementation strategies by practitioners and researchers undertaking work in drowning prevention in HICs and supports a call to action for better documentation of implementation in public health interventions.
Copy Number Variations in Pancreatic Cancer: From Biological Significance to Clinical Utility
Authors: Oketch Daisy J. A. , Giulietti Matteo , Piva Francesco
Published: December 2023

Pancreatic ductal adenocarcinoma (PDAC) is the most common type of pancreatic cancer, characterized by high tumor heterogeneity and a poor prognosis. Inter- and intra-tumoral heterogeneity in PDAC is a major obstacle to effective PDAC treatment; therefore, it is highly desirable to explore the tumor heterogeneity and underlying mechanisms for the improvement of PDAC prognosis. Gene copy number variations (CNVs) are increasingly recognized as a common and heritable source of inter-individual variation in genomic sequence. In this review, we outline the origin, main characteristics, and pathological aspects of CNVs. We then describe the occurrence of CNVs in PDAC, including those that have been clearly shown to have a pathogenic role, and further highlight some key examples of their involvement in tumor development and progression. The ability to efficiently identify and analyze CNVs in tumor samples is important to support translational research and foster precision oncology, as copy number variants can be utilized to guide clinical decisions. We provide insights into understanding the CNV landscapes and the role of both somatic and germline CNVs in PDAC, which could lead to significant advances in diagnosis, prognosis, and treatment. Although there has been significant progress in this field, understanding the full contribution of CNVs to the genetic basis of PDAC will require further research, with more accurate CNV assays such as single-cell techniques and larger cohorts than have been performed to date.
Synovial Fluid from Patients with Osteoarthritis Shows Different Inflammatory Features Depending on the Presence of Calcium Pyrophosphate Crystals
Authors: Oliviero Francesca , Baggio Chiara , Favero Marta , Damasco Amelia Carmela , Boscaro Carlotta , Tietto Davide , Albiero Mattia , Doria Andrea , Ramonda Roberta
Published: December 2023

The role of calcium pyrophosphate (CPP) crystals in osteoarthritis (OA) is still a matter of debate. With this study we aimed to investigate the inflammatory features of synovial fluid (SF) collected from patients with OA with CPP crystals compared with those without crystals. We also explored the effect of OA SF on monocytes response. SFs were collected from adult patients with OA and subdivided according to the presence of crystals. Local cellular and humoral inflammatory mediators were analysed in the SF samples. The expression levels of IL-1β, IL-18, CASP-1, NLRP3, and GAPDH were measured by RT-PCR in the cells obtained by pelleting the SF samples. For the in vitro study, a monocytic cell line was treated with selected SF samples. SF with CPP crystals showed a significant increase in inflammatory cellular indices and higher levels of IL-1β, IL-8, and caspase-1 transcript with respect to SF without crystals. Higher concentrations of VEGF were also observed in the early stages of the whole OA patients. THP-1 cells stimulated with OA SF released a significant amount of IL-1 β in culture supernatants. This study demonstrated that SF collected from patients with OA shows different inflammatory features depending on the presence of CPP crystals.
Conserved Residues Lys64 and Glu78 at the Subunit Surface of Tau Glutathione Transferase in Rice Affect Structure and Enzymatic Properties
Authors: Yang Xue , Zhang Zhe , Wu Lei , Yang Meiying , Li Siyuan , Gao Jie
Published: December 2023

Glutathione transferases (GSTs) are a superfamily of dimeric proteins associated with the detoxification of various reactive electrophiles and responsive to a multitude of stressors. We individually substituted Lys64 and Glu78 with Ala using site-directed mutagenesis to understand the role of subunit interactions in the structure and enzymatic properties of a rice GST (OsGSTU17). The wild-type OsGSTU17 lost the conserved hydrogen bond between subunits in tau class GSTs due to conserved Tyr92 replaced with Phe92, but still exhibited high substrate activities, and thermal stability remained in its dimeric structure. The significant decrease in thermal stability and obvious changes in the structure of mutant K64A implied that conserved Lys64 might play an essential role in the structural stability of tau class GSTs. The mutant E78A, supposed to be deprived of hydrogen and salt bonds between subunits, appeared in the soluble form of dimers, even though its tertiary structure altered and stability declined dramatically. These results suggest that the hydrogen and ionic bonds provided by conserved residues are not as important for OsGSTU17 dimerization and enzymatic properties. These results further supplement our understanding of the relationship between the structure and function of GSTs and provide a theoretical basis for improving crop resistance through targeted modification of GSTs.
Unique tRNA Fragment Upregulation with SARS-CoV-2 but Not with SARS-CoV Infection
Authors: Imirowicz Isabella , Saifee Azeem , Henry Leanne , Tunkle Leo , Popescu Alexander , Huang Philip , Jakpor Jibiana , Barbano Ava , Goru Rohit , Gunawan Audrey , Sicilia Maria , Ono Mori , Bao Xiaoyong , Lee Inhan
Published: December 2023

Unlike other coronaviruses, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has rapidly infected the global population, with some suffering long-term effects. Thanks to extensive data on SARS-CoV-2 made available through global, multi-level collaborative research, investigators are getting closer to understanding the mechanisms of SARS-CoV-2 infection. Here, using publicly available total and small RNAseq data of Calu3 cell lines, we conducted a comparative analysis of the changes in tRNA fragments (tRFs; regulatory small noncoding RNAs) in the context of severe acute respiratory syndrome coronavirus (SARS-CoV) and SARS-CoV-2 infections. We found extensive upregulation of multiple tRFs in SARS-CoV-2 infection that was not present in SARS-CoV or other virus infections our group has studied. By comparing the total RNA changes in matching samples, we identified significant downregulation of TRDMT1 (tRNA methyltransferase), only in SARS-CoV-2 infection, a potential upstream event. We further found enriched neural functions among downregulated genes with SARS-CoV-2 infection. Interestingly, theoretically predicted targets of the upregulated tRFs without considering mRNA expression data are also enriched in neural functions such as axon guidance. Based on a combination of expression data and theoretical calculations, we propose potential targets for tRFs. For example, among the mRNAs downregulated with SARS-CoV-2 infection (but not with SARS-CoV infection), SEMA3C is a theoretically calculated target of multiple upregulated tRFs and a ligand of NRP1, a SARS-CoV-2 receptor. Our analysis suggests that tRFs contribute to distinct neurological features seen in SARS-CoV-2.
Synthesis and Future Electronic Applications of Topological Nanomaterials
Authors: Jin Gangtae , Kim Seo-Hyun , Han Hyeuk-Jin
Published: December 2023

Over the last ten years, the discovery of topological materials has opened up new areas in condensed matter physics. These materials are noted for their distinctive electronic properties, unlike conventional insulators and metals. This discovery has not only spurred new research areas but also offered innovative approaches to electronic device design. A key aspect of these materials is now that transforming them into nanostructures enhances the presence of surface or edge states, which are the key components for their unique electronic properties. In this review, we focus on recent synthesis methods, including vapor–liquid–solid (VLS) growth, chemical vapor deposition (CVD), and chemical conversion techniques. Moreover, the scaling down of topological nanomaterials has revealed new electronic and magnetic properties due to quantum confinement. This review covers their synthesis methods and the outcomes of topological nanomaterials and applications, including quantum computing, spintronics, and interconnects. Finally, we address the materials and synthesis challenges that need to be resolved prior to the practical application of topological nanomaterials in advanced electronic devices.
The Transcription Factor StuA Regulates the Glyoxylate Cycle in the Dermatophyte Trichophyton rubrum under Carbon Starvation
Authors: Petrucelli Monise Fazolin , Martins-Santana Leonardo , Sanches Pablo R. , Oliveira Vanderci M. , Rossi Antonio , Martinez-Rossi Nilce M.
Published: December 2023

Trichophyton rubrum is the primary causative agent of dermatophytosis worldwide. This fungus colonizes keratinized tissues and uses keratin as a nutritional source during infection. In T. rubrum–host interactions, sensing a hostile environment triggers the adaptation of its metabolic machinery to ensure its survival. The glyoxylate cycle has emerged as an alternative metabolic pathway when glucose availability is limited; this enables the conversion of simple carbon compounds into glucose via gluconeogenesis. In this study, we investigated the impact of stuA deletion on the response of glyoxylate cycle enzymes during fungal growth under varying culture conditions in conjunction with post-transcriptional regulation through alternative splicing of the genes encoding these enzymes. We revealed that the ΔstuA mutant downregulated the malate synthase and isocitrate lyase genes in a keratin-containing medium or when co-cultured with human keratinocytes. Alternative splicing of an isocitrate lyase gene yielded a new isoform. Enzymatic activity assays showed specific instances where isocitrate lyase and malate synthase activities were affected in the mutant strain compared to the wild type strain. Taken together, our results indicate a relevant balance in transcriptional regulation that has distinct effects on the enzymatic activities of malate synthase and isocitrate lyase.
Long COVID: Molecular Mechanisms and Detection Techniques
Authors: Constantinescu-Bercu Adela , Lobiuc Andrei , Căliman-Sturdza Olga Adriana , Oiţă Radu Cristian , Iavorschi Monica , Pavăl Naomi-Eunicia , Șoldănescu Iuliana , Dimian Mihai , Covasa Mihai
Published: December 2023

Long COVID, also known as post-acute sequelae of SARS-CoV-2 infection (PASC), has emerged as a significant health concern following the COVID-19 pandemic. Molecular mechanisms underlying the occurrence and progression of long COVID include viral persistence, immune dysregulation, endothelial dysfunction, and neurological involvement, and highlight the need for further research to develop targeted therapies for this condition. While a clearer picture of the clinical symptomatology is shaping, many molecular mechanisms are yet to be unraveled, given their complexity and high level of interaction with other metabolic pathways. This review summarizes some of the most important symptoms and associated molecular mechanisms that occur in long COVID, as well as the most relevant molecular techniques that can be used in understanding the viral pathogen, its affinity towards the host, and the possible outcomes of host-pathogen interaction.
Advantages of Induced Circular Dichroism Spectroscopy for Qualitative and Quantitative Analysis of Solution-Phase Cyclodextrin Host–Guest Complexes
Authors: Kraszni Márta , Balogh Balázs , Mándity István , Horváth Péter
Published: December 2023

The presence of a chiral or chirally perturbed chromophore in the molecule under investigation is a fundamental requirement for the appearance of a circular dichroism (CD) spectrum. For native and for most of the substituted cyclodextrins, this condition is not applicable, because although chiral, cyclodextrins lack a chromophore group and therefore have no characteristic CD spectra over 220 nm. The reason this method can be used is that if the guest molecule has a chromophore group and this is in the right proximity to the cyclodextrin, it becomes chirally perturbed. As a result, the complex will now provide a CD signal, and this phenomenon is called induced circular dichroism (ICD). The appearance of the ICD spectrum is clear evidence of the formation of the complex, and the spectral sign and intensity is a good predictor of the structure of the complex. By varying the concentration of cyclodextrin, the ICD signal changes, resulting in a saturation curve, and from these data, the stability constant can be calculated for a 1:1 complex. This article compares ICD and NMR spectroscopic and molecular modeling results of cyclodextrin complexes of four model compounds: nimesulide, fenbufen, fenoprofen, and bifonazole. The results obtained by the different methods show good agreement, and the structures estimated from the ICD spectra are supported by NMR data and molecular modeling.
SMC5 Plays Independent Roles in Congenital Heart Disease and Neurodevelopmental Disability
Authors: O’Brien Matthew P. , Pryzhkova Marina V. , Lake Evelyn M. R. , Mandino Francesca , Shen Xilin , Karnik Ruchika , Atkins Alisa , Xu Michelle J. , Ji Weizhen , Konstantino Monica , Brueckner Martina , Ment Laura R. , Khokha Mustafa K. , Jordan Philip W.
Published: December 2023

Up to 50% of patients with severe congenital heart disease (CHD) develop life-altering neurodevelopmental disability (NDD). It has been presumed that NDD arises in CHD cases because of hypoxia before, during, or after cardiac surgery. Recent studies detected an enrichment in de novo mutations in CHD and NDD, as well as significant overlap between CHD and NDD candidate genes. However, there is limited evidence demonstrating that genes causing CHD can produce NDD independent of hypoxia. A patient with hypoplastic left heart syndrome and gross motor delay presented with a de novo mutation in SMC5. Modeling mutation of smc5 in Xenopus tropicalis embryos resulted in reduced heart size, decreased brain length, and disrupted pax6 patterning. To evaluate the cardiac development, we induced the conditional knockout (cKO) of Smc5 in mouse cardiomyocytes, which led to the depletion of mature cardiomyocytes and abnormal contractility. To test a role for Smc5 specifically in the brain, we induced cKO in the mouse central nervous system, which resulted in decreased brain volume, and diminished connectivity between areas related to motor function but did not affect vascular or brain ventricular volume. We propose that genetic factors, rather than hypoxia alone, can contribute when NDD and CHD cases occur concurrently.