bluuebunny/crossref_metadata_2025 huggingface dataset:
Incomplete Pattern of Steroidogenic Protein Expression in Functioning Adrenocortical Carcinomas
Authors: Pereira Sofia S. , Costa Madalena M. , Gomez-Sanchez Celso E. , Monteiro Mariana P. , Pignatelli Duarte
Published: July 2020

Autonomous steroid secretion is a common feature of adrenocortical carcinomas (ACC), although not always clinically evident owing to inefficient steroidogenesis with increased release of steroid precursors. Our study aim was to analyze the expression profile of four key proteins involved in the steroidogenesis cascade, in different adrenocortical tumors. Expression of proteins involved in steroidogenesis, namely steroidogenic acute regulatory protein (StAR), 11β-hydroxylase (CYP11B1), aldosterone synthase (CYP11B2) and 17α-hydroxylase (CYP17A1), were analyzed by immunohistochemistry in ACC (n = 14), adenomas presenting with Cushing’s syndrome (ACAc) (n = 11) and clinically non-functioning adenomas (ACAn) (n = 15). A percentage of the stained area for each protein was analyzed using ImageJ software for computerized morphometric quantification. CYP11B1, StAR and CYP17A1 expression were significantly lower in ACC when compared to ACAc. In addition, ACC presented co-staining cells for CYP11B1 and CYP11B2. CYP11B1 was the steroidogenic enzyme with the most discriminative power to distinguish ACC from ACAc, with a sensitivity of 100%, specificity of 92%, and an expression higher than 4.44%, indicating the presence of a cortisol secreting adenoma. ACC depicts an incomplete pattern of steroidogenic protein expression, with decreased CYP11B1 and CYP17A1, which could explain the predominant secretion of steroid precursors.
The Inflammasome in Chronic Complications of Diabetes and Related Metabolic Disorders
Authors: Menini Stefano , Iacobini Carla , Vitale Martina , Pugliese Giuseppe
Published: July 2020

Diabetes mellitus (DM) ranks seventh as a cause of death worldwide. Chronic complications, including cardiovascular, renal, and eye disease, as well as DM-associated non-alcoholic fatty liver disease (NAFLD) account for most of the morbidity and premature mortality in DM. Despite continuous improvements in the management of late complications of DM, significant gaps remain. Therefore, searching for additional strategies to prevent these serious DM-related conditions is of the utmost importance. DM is characterized by a state of low-grade chronic inflammation, which is critical in the progression of complications. Recent clinical trials indicate that targeting the prototypic pro-inflammatory cytokine interleukin-1β (IL-1 β) improves the outcomes of cardiovascular disease, which is the first cause of death in DM patients. Together with IL-18, IL-1β is processed and secreted by the inflammasomes, a class of multiprotein complexes that coordinate inflammatory responses. Several DM-related metabolic factors, including reactive oxygen species, glyco/lipoxidation end products, and cholesterol crystals, have been involved in the pathogenesis of diabetic kidney disease, and diabetic retinopathy, and in the promoting effect of DM on the onset and progression of atherosclerosis and NAFLD. These metabolic factors are also well-established danger signals capable of regulating inflammasome activity. In addition to presenting the current state of knowledge, this review discusses how the mechanistic understanding of inflammasome regulation by metabolic danger signals may hopefully lead to novel therapeutic strategies targeting inflammation for a more effective treatment of diabetic complications.
RETRACTED: Hong et al. Acetylshikonin Sensitizes Hepatocellular Carcinoma Cells to Apoptosis through ROS-Mediated Caspase Activation. Cells 2019, 8, 1466
Authors:   Cells Editorial Office
Published: July 2020

The Editorial Office and the authors have taken the decision to retract the published article [...]
Effect of Functional Progressive Resistance Exercise on Lower Extremity Structure, Muscle Tone, Dynamic Balance and Functional Ability in Children with Spastic Cerebral Palsy
Authors: Cho Hye-Jin , Lee Byoung-Hee
Published: July 2020

The purpose of this study was to investigate the effects of functional progressive resistance exercise (FPRE) on muscle tone, dynamic balance and functional ability in children with spastic cerebral palsy. Twenty-five subjects were randomized into two groups: the FPRE group (n = 13) and the control group (n = 12). The experimental group participated in an FPRE program for 30 min per day, three times per week for six weeks. Knee extensor strength, rehabilitative ultrasound imaging (RUSI), muscle tone, dynamic balance, and functional ability was evaluated. The results showed statistically significant time × group interaction effects on the dominant side for knee extensor strength and cross-sectional area (CSA) in RUSI (p < 0.05). On both sides for thickness of the quadriceps (TQ) in RUSI, muscle tone and dynamic balance were statistically significant time × group interaction effects (p < 0.05). Additionally, knee extensor strength, CSA, TQ in RUS, muscle tone, dynamic balance and gross motor function measure (GMFM) in functional ability were significantly increased between pre- and post-intervention within the FPRE group (p < 0.05). The results suggest that FPRE is both feasible and beneficial for improving muscle tone, dynamic balance and functional ability in children with spastic cerebral palsy.
Case Study of Chosen Sandwich-Structured Composite Materials for Means of Transport
Authors: Koštial Pavel , Koštialová Jančíková Zora , Ružiak Ivan , Gajtanska Milada
Published: July 2020

Modern means of transport increasingly utilize sandwich constructions. Among other things, the reasons for such state of affairs include the reduced weight of means of transport, and through this, better fuel economy as well as price. This work is dedicated to a systematic experimental study of the influence of various materials and sandwich designs on their mechanical properties. In the framework of experiments, sandwich-structured composites were exposed to two types of stressing: static as well as impact stressing. The testing of prepared samples was performed according to ASTM C-393 Standard, dealing specifically with the bending behavior of sandwich composite constructions and impact testing under the scope of ISO 6603-2 Standard test. In this article we deal with static and impact testing of the eight types of core materials, two types of coatings, two types of surface finishes, and two types of resins with a special emphasis on their use in constructions of some exterior or interior components of transport means.
Multi-Slot BLE Raw Database for Accurate Positioning in Mixed Indoor/Outdoor Environments
Authors: Aranda Fernando J. , Parralejo Felipe , Álvarez Fernando J. , Torres-Sospedra Joaquín
Published: July 2020

The technologies and sensors embedded in smartphones have contributed to the spread of disruptive applications built on top of Location Based Services (LBSs). Among them, Bluetooth Low Energy (BLE) has been widely adopted for proximity and localization, as it is a simple but efficient positioning technology. This article presents a database of received signal strength measurements (RSSIs) on BLE signals in a real positioning system. The system was deployed on two buildings belonging to the campus of the University of Extremadura in Badajoz. the database is divided into three different deployments, changing in each of them the number of measurement points and the configuration of the BLE beacons. the beacons used in this work can broadcast up to six emission slots simultaneously. Fingerprinting positioning experiments are presented in this work using multiple slots, improving positioning accuracy when compared with the traditional single slot approach.
Clinical Feasibility of Reduced Field-of-View Diffusion-Weighted Magnetic Resonance Imaging with Computed Diffusion-Weighted Imaging Technique in Breast Cancer Patients
Authors: Cho Eun , Lee Jin Hwa , Baek Hye Jin , Ha Ji Young , Ryu Kyeong Hwa , Park Sung Eun , Moon Jin Il , Gho Sung-Min , Wakayama Tetsuya
Published: July 2020

Background: We evaluated the feasibility of the reduced field-of-view (rFOV) diffusion-weighted imaging (DWI) with computed DWI technique by comparison and analysis of the inter-method agreement among acquired rFOV DWI (rFOVA), rFOV DWI with computed DWI technique (rFOVS), and dynamic contrast-enhanced (DCE) magnetic resonance imaging (MRI) in patients with breast cancer. Methods: A total of 130 patients with biopsy-proven breast cancers who underwent breast MRI from April 2017 to December 2017 were included in this study. The rFOVS were reformatted by calculation of the apparent diffusion coefficient curve obtained from rFOVA b = 0 s/mm2 and b = 500 s/mm2. Visual assessment of the image quality of rFOVA b = 1000 s/mm2, rFOVS, and DCE MRI was performed using a four-point grading system. Morphologic analyses of the index cancer was performed on rFOVA, rFOVS, and DCE MRI. The signal-to-noise ratio (SNR), contrast-to-noise ratio (CNR), and contrast of tumor-to-parenchyma (TPC) were calculated. Results: Image quality scores with rFOVA, rFOVS, and DCE MRI were not significantly different (p = 0.357). Lesion analysis of shape, margin, and size of the index cancer also did not show significant differences among the three sequences (p = 0.858, p = 0.242, and p = 0.858, respectively). SNR, CNR, and TPC of DCE MRI were significantly higher than those of rFOVA and rFOVS (p < 0.001, p = 0.001, and p = 0.016, respectively). Significant differences were not found between the SNR, CNR, and TPC of rFOVA and those of rFOVS (p > 0.999, p > 0.999, and p > 0.999, respectively). Conclusion: The rFOVA and rFOVS showed nearly equivalent levels of image quality required for morphological analysis of the tumors and for lesion conspicuity compared with DCE MRI.
Stochastic Comparisons of Weighted Distributions and Their Mixtures
Authors: Albabtain Abdulhakim A. , Shrahili Mansour , Al-Shehri M. A. , Kayid M.
Published: July 2020

In this paper, various stochastic ordering properties of a parametric family of weighted distributions and the associated mixture model are developed. The effect of stochastic variation of the output random variable with respect to the parameter and/or the underlying random variable is specifically investigated. Special weighted distributions are considered to scrutinize the consistency as well as the usefulness of the results. Stochastic comparisons of coherent systems made of identical but dependent components are made and also a result for comparison of Shannon entropies of weighted distributions is developed.
Postgraduate Studies on Lean Management—A Review of Initiatives
Authors: Bednarek Mariusz , Buczacki Aleksander , Bielakowski Lukasz , Gladysz Bartlomiej , Bryke Mariusz
Published: July 2020

The article elaborates upon a successful model of postgraduate studies on lean manufacturing. The subject of the research was nine editions of the Kaizen Academy postgraduate studies organized by the Warsaw University of Technology and the Kaizen Institute in the years 2009–2018, that still enjoys interest in the market. A detailed qualitative analysis of this case, followed by a comparative analysis with other post-graduate programs of this type on the national, European, and global level should make it possible to find what is necessary to launch and actively operate for a long time in the (considerably competitive) market of qualification raising programs related to Lean Management (critical success factors). The study has also identified the differences between a successful program, analyzed in detail, and other available programs described in the literature and on the Internet. This has been achieved through a structured analysis of thirty-nine postgraduate programs identified by querying scientific databases and the Google Internet search engine. Best practices and models of postgraduate education on Lean Management have been discussed, and the thirty-nine programs comprising the identified sample have been compared. Considering the very general level of the data available in terms of the organization of postgraduate programs, it has also been assessed if they follow constructivism and experiential learning paradigms. This has been achieved by including the industrial days/visit indicator in the comparison. Given its high value, it has been identified as a success factor of the postgraduate curriculum on Lean Management.
A Constrained Non-Linear Model Predictive Controller for the Rotor Flux-Oriented Control of an Induction Motor Drive
Authors: Rosa Fabiano C. , Bim Edson
Published: July 2020

Predictive controllers have been extensively studied and applied to electrical drives, mainly because they provide fast dynamic responses and are suitable for multi-variable control and non-linear systems. Many approaches perform the prediction and optimization process on-line, which requires a high computational capacity for fast dynamics, such as, for example, the control of AC electric motors. Due to the complexity of embedding constraints in controller design, which demands a high computational capacity to solve the optimization problem, off-line approaches are one of the choices to overcome this problem. However, these strategies do not deal with the inherent constraints of the drive system, which significantly simplifies the design of the controller. This paper proposes a non-linear and multi-variable predictive controller to control the speed and rotor flux of an induction motor, where the constraints are treated after the controller design. Besides dealing with the constraints of the electric drive system, our proposal allows increasing the stability of the system when the model does not incorporate disturbances and when parameter incompatibilities occur. Several computer simulations and experimental tests were performed to evaluate the behavior of the proposed controller, showing good performance to track the controlled variables under normal operating conditions, under load disturbances, parametric incompatibility, and at a very low rotor speed.
Numeric Simulation of Acoustic-Logging of Cave Formations
Authors: Xu Fanghui , Wang Zhuwen
Published: July 2020

The finite difference (FD) method of monopole source is used to simulate the response of full-wave acoustic-logging in cave formations. The effect of the cave in the formation of borehole full-waves was studied. The results show that the radius of cave is not only linearly related to the first arrival of the compressional wave (P-wave), but also to the energy of the shear wave (S-wave). The converted S (S–S wave) and P-waves (S–P wave) are formed when the S-wave encounters the cave. If the source distance is small, the S–S and S–P waves are not separated, and the attenuation of the S-wave is not large, due to superposition of the converted waves. The S–P wave has been separated from the S-wave when the source distance is large, so the attenuation of the S-wave increases. The amplitude of the P and S–waves changes most when the distance of the cave to the borehole wall reaches a certain value; this value is related to the excitation frequency. The amplitude of the Stoneley wave (ST wave) varies directly with the radius of cave. If the radius of the cave is large, the energy of ST wave is weak. The scattered wave is determined by the radius and position of the cave. The investigation depth of a monopole source is limited. When the distance of the cave to the borehole wall exceeds the maximum investigation depth, the borehole acoustic wave is little affected by the cave. In actual logging, the development of the cave can be evaluated by using the first arrival of the P-wave and the energy of the S and ST waves.
Fire as a Selection Agent for the Dissemination of Invasive Species: Case Study on the Evolution of Forest Coverage
Authors: Nunes Leonel J. R. , Raposo Mauro A. M. , Meireles Catarina I. R. , Pinto Gomes Carlos J. , Ribeiro Nuno M. C. Almeida
Published: July 2020

Climate change has enhanced the occurrence of rural fires, since changes in the hydrological cycle have led to the occurrence of increasingly long and frequent periods of drought. This recurrence of rural fires in Portugal, in turn, has led to the successive elimination of vast areas traditionally occupied by native species or species of economic interest, which are being successively replaced by new species with invasive behavior. Among these, Acacia dealbata stands out for its dispersion capacity and for the area it has already occupied. In the present work, which reviews the evolution of forest cover over the last 18,000 years in the Serra da Estrela Natural Park, we intend to demonstrate that fire acts as a species selection agent and that it enhances the development of heliophile and pyrophyte species. For this purpose, an area of the municipality of Seia was selected, more specifically Casal do Rei, where the development of Acacia dealbata forests is monitored. In the end, it was concluded that, in fact, by analyzing the ages of the specimens present in these populations, fire acts as a selection agent by freeing up the space previously occupied by other species, opening the way for the growth of heliophiles and pyrophytes invasive species while enhancing their germination.
Epigenomic Remodeling in Huntington’s Disease—Master or Servant?
Authors: Zimmer-Bensch Geraldine
Published: July 2020

In light of our aging population, neurodegenerative disorders are becoming a tremendous challenge, that modern societies have to face. They represent incurable, progressive conditions with diverse and complex pathological features, followed by catastrophic occurrences of massive neuronal loss at the later stages of the diseases. Some of these disorders, like Huntington’s disease (HD), rely on defined genetic factors. HD, as an incurable, fatal hereditary neurodegenerative disorder characterized by its mid-life onset, is caused by the expansion of CAG trinucleotide repeats coding for glutamine (Q) in exon 1 of the huntingtin gene. Apart from the genetic defect, environmental factors are thought to influence the risk, onset and progression of HD. As epigenetic mechanisms are known to readily respond to environmental stimuli, they are proposed to play a key role in HD pathogenesis. Indeed, dynamic epigenomic remodeling is observed in HD patients and in brains of HD animal models. Epigenetic signatures, such as DNA methylation, histone variants and modifications, are known to influence gene expression and to orchestrate various aspects of neuronal physiology. Hence, deciphering their implication in HD pathogenesis might open up new paths for novel therapeutic concepts, which are discussed in this review.
A New Fabric Reinforced Geopolymer Mortar (FRGM) with Mechanical and Energy Benefits
Authors: Longo Fabio , Cascardi Alessio , Lassandro Paola , Aiello Maria Antonietta
Published: July 2020

A large part of the European building Heritage is dated back over centuries. Consequently, its structural and thermal performances are often inadequate. Commonly, different interventions are proposed for solving these issues separately. However, reasonable drawbacks arise when the structural retrofitting requires a direct contact with the target-member while the insulation layer is potentially interposed in between. In this scenario, the present research proposes a novel and unique system able to guarantee both the energetic and the structural retrofitting. Inorganic Matrix Composites (IMCs) are a promising solution in this sense. Among them, the Fabric Reinforced Cementitous Matrix (FRCM) is one of the most used; or rather a composite made of a fabric (open grid or mesh) within an inorganic matrix (lime or cement based). Even if the inorganic matrix has a relevant thickness (if compared with the one of the fabric), its thermal resistance is insufficient. The novelty of this work consists in assessing a new geo-polymeric FRCM-system by combining fly-ash binder (reused material) and expanded glass aggregate (recycled material). Direct tensile tests, for measuring the tensile strength, ultimate strain and elastic modulus, were performed in addition to thermal conductivity tests. The results were compared with those of traditional FRCM (commercially available). The potentiality of the proposal for structural and energy retrofitting is discussed and examples of its possible application are also reported.
Game Related Statistics Discriminate National and Foreign Players According to Playing Position and Team Ability in the Women’s Basketball EuroLeague
Authors: Gasperi Lorenzo , Conte Daniele , Leicht Anthony , Gómez-Ruano Miguel-Ángel
Published: July 2020

The aim of the present study was to examine the differences in game-related statistics between national and foreign female basketball players in the Women’s EuroLeague, according to playing positions and team ability. The official box-scores of 112 games from the 2016–2017 season of the Women’s EuroLeague (FIBA) were examined. Players were categorised based upon country of nationality versus competition (i.e., foreign or national), playing positions (i.e., Guards, Forwards, Centers), and team ability (i.e., four groups using a cluster of k-means analysis according to the winning percentage of each team during the competition). A structural coefficient (SC) above |0.30| was used to identify the variables that best differentiated the national and foreign players. Results showed that foreign players had a better performance according to team ability and playing position for most of the performance indicators, with higher values for minutes played, percentage of successful 2-point field-goals, percentage of successful free-throws, and percentage of assists. Moreover, foreign players performed better in variables associated with offensive situations, while national players were prevailing with indicators associated with defensive actions. These results have highlighted the unique contributions of foreign and national players, based upon playing position and team ability, to team success in the Euroleague. This information will assist the recruitment process of national and foreign athletes for coaches to develop successful elite female basketball teams.
Modelling the Effectiveness of Epidemic Control Measures in Preventing the Transmission of COVID-19 in Malaysia
Authors: Gill Balvinder Singh , Jayaraj Vivek Jason , Singh Sarbhan , Mohd Ghazali Sumarni , Cheong Yoon Ling , Md Iderus Nuur Hafizah , Sundram Bala Murali , Aris Tahir Bin , Mohd Ibrahim Hishamshah , Hong Boon Hao , Labadin Jane
Published: July 2020

Malaysia is currently facing an outbreak of COVID-19. We aim to present the first study in Malaysia to report the reproduction numbers and develop a mathematical model forecasting COVID-19 transmission by including isolation, quarantine, and movement control measures. We utilized a susceptible, exposed, infectious, and recovered (SEIR) model by incorporating isolation, quarantine, and movement control order (MCO) taken in Malaysia. The simulations were fitted into the Malaysian COVID-19 active case numbers, allowing approximation of parameters consisting of probability of transmission per contact (β), average number of contacts per day per case (ζ), and proportion of close-contact traced per day (q). The effective reproduction number (Rt) was also determined through this model. Our model calibration estimated that (β), (ζ), and (q) were 0.052, 25 persons, and 0.23, respectively. The (Rt) was estimated to be 1.68. MCO measures reduce the peak number of active COVID-19 cases by 99.1% and reduce (ζ) from 25 (pre-MCO) to 7 (during MCO). The flattening of the epidemic curve was also observed with the implementation of these control measures. We conclude that isolation, quarantine, and MCO measures are essential to break the transmission of COVID-19 in Malaysia.
Abelmoschus Esculentus (L.) Moench’s Peel Powder Improves High-Fat-Diet-Induced Cognitive Impairment in C57BL/6J Mice
Authors: Prom-in Supattra , Kaewsrichan Jasadee , Wangpradit Nuntika , Kien Hui Chua , Yahaya Mohamad Fairuz , Kamisah Yusof , Kumar Jaya
Published: July 2020

Okra peel exhibits numerous therapeutic effects. This study explores the potential ameliorative effects of okra peel powder on high-fat-diet (HFD)-induced hypercholesterolemia and cognitive deficits. Thirty-six C57BL/6J male mice were randomly divided into six groups (n = 6 per group): (i) control, mice fed with a normal diet; (ii) HFD, mice fed with HFD; (iii) HFD-SIM, mice fed with HFD and given simvastatin (20 mg/kg/day); (iv) HFD-OP1; (v) HFD-OP2; (vi) HFD-OP3, mice fed with HFD and okra peel (200, 400, or 800 mg/kg/day, respectively). Following 10 weeks of treatments, the mice were subjected to the Morris water maze (MWM). Parameters such as weekly average body weight, food intake, and blood lipid profiles were also recorded. The HFD group showed a profound increase in total cholesterol and low-density lipoprotein concentration compared to the control group. All okra-treated and HFD-SIM groups performed better than the HFD group during acquisition trials, whereas only the HFD-OP1 produced a significantly higher number of entries into the platform zone during the probe trial. In sum, all three okra doses improved the learning ability of the mice. However, only the lowest dose of okra significantly improved the spatial reference memory retention.
Symptoms of Posttraumatic Stress, Anxiety, Depression, Levels of Resilience and Burnout in Spanish Health Personnel during the COVID-19 Pandemic
Authors: Luceño-Moreno Lourdes , Talavera-Velasco Beatriz , García-Albuerne Yolanda , Martín-García Jesús
Published: July 2020

The number of health workers infected with COVID-19 in Spain is one of the highest in the world. The aim of this study is to analyse posttraumatic stress, anxiety and depression during the COVID-19 pandemic. Associations between burnout, resilience, demographic, work and COVID-19 variables are analysed. Cross-sectional data on 1422 health workers were analysed. A total of 56.6% of health workers present symptoms of posttraumatic stress disorder, 58.6% anxiety disorder, 46% depressive disorder and 41.1% feel emotionally drained. The profile of a health worker with greater posttraumatic stress symptoms would be a person who works in the Autonomous Community of Madrid, in a hospital, is a woman, is concerned that a person he/she lives with may be infected, and thinks that he/she is very likely to be infected. The risk variables for anxiety and depression would be a person that is a woman, working 12- or 24-h shifts, and being worried that a family member could be infected. High scores on emotional exhaustion and depersonalization are risk factors for mental health, with resilience and personal fulfilment being protective variables. Data are provided to improve preventive measures for occupational health workers.
The Apple of Daddy’s Eye: Parental Overvaluation Links the Narcissistic Traits of Father and Child
Authors: Coppola Gabrielle , Musso Pasquale , Buonanno Carlo , Semeraro Cristina , Iacobellis Barbara , Cassibba Rosalinda , Levantini Valentina , Masi Gabriele , Thomaes Sander , Muratori Pietro
Published: July 2020

This study contributes to the literature on the parental correlates of children’s narcissism. It addresses whether parental overvaluation may drive the putative link between parents’ narcissism and children’s narcissism and self-esteem. The cross-sectional design involved a community sample of 519 school-age children (age ranging from 9 to 11 years old) and their parents from an Italian urban context. Child-reported measures included narcissistic traits and self-esteem, while parent-reported measures included narcissistic traits and overvaluation, as well as parenting styles. A series of structural equation models, run separately for mothers and fathers, showed that both parents’ narcissism was directly and positively related to overvaluation and the children’s narcissistic traits; overvaluation partially mediated the indirect link between the fathers’ and children’s narcissistic traits. None of the parenting-style dimensions were related to the children’s outcomes, with the exception of the mothers’ positive parenting being directly and positively related to children’s self-esteem. These findings shed new light upon the parental correlates of child narcissism by suggesting that mothers and fathers convey their narcissism to their offspring through differential pathways. Our findings may be understood from universal as well as cultural specifics regarding the parenting roles of mothers and fathers. Clinical implications for the treatment of youth narcissism suggest the potential of targeting not only children but also their parents.
The Association of Cooking Fuel Use, Dietary Intake, and Blood Pressure among Rural Women in China
Authors: Bellows Alexandra L. , Spiegelman Donna , Du Shufa , Jaacks Lindsay M.
Published: July 2020

Household air pollution (HAP) from solid cooking fuels continues to affect 600 million people in China and has been associated with high blood pressure. The role of diet in HAP-associated high blood pressure has yet to be evaluated in China. The aim of this study was to estimate the impact of cooking fuel on change in blood pressure and evaluate whether intake of antioxidant- and omega-3 fatty acid-rich foods (fruits, vegetables, and seafood) attenuates any adverse effects of solid fuel use on blood pressure. We analyzed longitudinal data collected between 1991 and 2011 from nonpregnant women aged 18 to 80 years living in rural areas of China. We used linear mixed effects models to estimate the association between cooking fuel (coal or wood versus clean [electric or liquid petroleum gas]) and blood pressure. Possible mediation of the fuel effect by diet was assessed by the difference method. A total of 6671 women were included in this study. Women less than 40 years of age cooking with cleaner fuels over time had lower rates of change in systolic blood pressure compared to women cooking with coal (p = 0.004), and this effect was not mediated by dietary intake. Associations between fuel use and change in diastolic blood pressure were not significant. These findings lend further support for there being a direct effect of reducing HAP on improvements in blood pressure, independent of concurrent dietary intake.
Experiences of Nursing Students during the Abrupt Change from Face-to-Face to e-Learning Education during the First Month of Confinement Due to COVID-19 in Spain
Authors: Ramos-Morcillo Antonio Jesús , Leal-Costa César , Moral-García José Enrique , Ruzafa-Martínez María
Published: July 2020

The current state of alarm due to the COVID-19 pandemic has led to the urgent change in the education of nursing students from traditional to distance learning. The objective of this study was to discover the learning experiences and the expectations about the changes in education, in light of the abrupt change from face-to-face to e-learning education, of nursing students enrolled in the Bachelor’s and Master’s degree of two public Spanish universities during the first month of confinement due to the COVID-19 pandemic. Qualitative study was conducted during the first month of the state of alarm in Spain (from 25 March–20 April 2020). Semi-structured interviews were given to students enrolled in every academic year of the Nursing Degree, and nurses who were enrolled in the Master’s programs at two public universities. A maximum variation sampling was performed, and an inductive thematic analysis was conducted. The study was reported according with COREQ checklist. Thirty-two students aged from 18 to 50 years old participated in the study. The interviews lasted from 17 to 51 min. Six major themes were defined: (1) practicing care; (2) uncertainty; (3) time; (4) teaching methodologies; (5) context of confinement and added difficulties; (6) face-to-face win. The imposition of e-learning sets limitations for older students, those who live in rural areas, with work and family responsibilities and with limited electronic resources. Online education goes beyond a continuation of the face-to-face classes. Work should be done about this for the next academic year as we face an uncertain future in the short-term control of COVID-19.
A Systematic Review of the Psychosocial Adjustment of Children and Adolescents with Facial Palsy: The Impact of Moebius Syndrome
Authors: Hotton Matthew , Huggons Esme , Hamlet Claire , Bogart Kathleen , Johnson David , Norris Jonathan H. , Kilcoyne Sarah , Dalton Louise
Published: July 2020

Introduction: Facial palsy is often associated with impaired facial function and altered appearance. However, the literature with regards to the psychological adjustment of children and adolescents with facial palsy has not been systematically reviewed to date. This paper aimed to review all published research with regards to psychosocial adjustment for children and adolescents with facial palsy. Methods: MEDLINE, CINAHL, Embase, PsychInfo and AMED databases were searched and data was extracted with regards to participant characteristics, study methodology, outcome measures used, psychosocial adjustment and study quality. Results: Five studies were eligible for inclusion, all of which investigated psychosocial adjustment in participants with Moebius syndrome, a form of congenital facial palsy. Many parents reported their children to have greater social difficulties than general population norms, with difficulties potentially increasing with age. Other areas of psychosocial adjustment, including behaviour, anxiety and depression, were found to be more comparable to the general population. Discussion: Children and adolescents with Moebius syndrome may experience social difficulties. However, they also demonstrate areas of resilience. Further research including individuals with facial palsy of other aetiologies is required in order to determine the psychosocial adjustment of children and adolescents with facial palsy.
Does Scale and Efficiency of Government Health Expenditure Promote Development of the Health Industry?
Authors: Wang Mengying , Gilmour Stuart , Tao Chunhai , Zhuang Kaixuan
Published: July 2020

Macro-economic development of China’s health industry is essential to the sustainable development and growth momentum of the national economy. Strategies to promote the development and rebalancing of the industrial structure need to be improved in order to transform China’s health industry and drive development. Based on panel data of 25 regions in China from 2004 to 2016, this paper analyzes the linear and non-linear relationship between Chinese government health expenditure (GHE), GHE efficiency, and the macro-economic development of the health industry. It uses a novel index of industrial structure to measure the transformation of industrial sectors in China, based on a semi-parametric generalized additive model. The model shows that per capita GHE and its efficiency have a significant positive linear and comprehensive non-linear effect on the development of health industry structure. By analyzing the interaction of GHE and its efficiency, we show that high expenditure with low-efficiency regimes and high expenditure with high-efficiency regimes have a positive impact on the development of industrial structure. Following the empirical results, the paper puts forward corresponding policy suggestions for the role of fiscal policy in promoting the development of the health industry in China.
Application of Coordination Compounds with Transition Metal Ions in the Chemical Industry—A Review
Authors: Malinowski Jacek , Zych Dominika , Jacewicz Dagmara , Gawdzik Barbara , Drzeżdżon Joanna
Published: July 2020

This publication presents the new trends and opportunities for further development of coordination compounds used in the chemical industry. The review describes the influence of various physicochemical factors regarding the coordination relationship (for example, steric hindrance, electron density, complex geometry, ligand), which condition technological processes. Coordination compounds are catalysts in technological processes used during organic synthesis, for example: Oxidation reactions, hydroformylation process, hydrogenation reaction, hydrocyanation process. In this article, we pointed out the possibilities of using complex compounds in catalysis, and we noticed what further research should be undertaken for this purpose.
Tissue-Specific Decellularization Methods: Rationale and Strategies to Achieve Regenerative Compounds
Authors: Mendibil Unai , Ruiz-Hernandez Raquel , Retegi-Carrion Sugoi , Garcia-Urquia Nerea , Olalde-Graells Beatriz , Abarrategi Ander
Published: July 2020

The extracellular matrix (ECM) is a complex network with multiple functions, including specific functions during tissue regeneration. Precisely, the properties of the ECM have been thoroughly used in tissue engineering and regenerative medicine research, aiming to restore the function of damaged or dysfunctional tissues. Tissue decellularization is gaining momentum as a technique to obtain potentially implantable decellularized extracellular matrix (dECM) with well-preserved key components. Interestingly, the tissue-specific dECM is becoming a feasible option to carry out regenerative medicine research, with multiple advantages compared to other approaches. This review provides an overview of the most common methods used to obtain the dECM and summarizes the strategies adopted to decellularize specific tissues, aiming to provide a helpful guide for future research development.
Antimicrobial Activity of Small Synthetic Peptides Based on the Marine Peptide Turgencin A: Prediction of Antimicrobial Peptide Sequences in a Natural Peptide and Strategy for Optimization of Potency
Authors: Hansen Ida K. Ø. , Lövdahl Tomas , Simonovic Danijela , Hansen Kine Ø. , Andersen Aaron J. C. , Devold Hege , Richard Céline S. M. , Andersen Jeanette H. , Strøm Morten B. , Haug Tor
Published: July 2020

Turgencin A, a potent antimicrobial peptide isolated from the Arctic sea squirt Synoicum turgens, consists of 36 amino acid residues and three disulfide bridges, making it challenging to synthesize. The aim of the present study was to develop a truncated peptide with an antimicrobial drug lead potential based on turgencin A. The experiments consisted of: (1) sequence analysis and prediction of antimicrobial potential of truncated 10-mer sequences; (2) synthesis and antimicrobial screening of a lead peptide devoid of the cysteine residues; (3) optimization of in vitro antimicrobial activity of the lead peptide using an amino acid replacement strategy; and (4) screening the synthesized peptides for cytotoxic activities. In silico analysis of turgencin A using various prediction software indicated an internal, cationic 10-mer sequence to be putatively antimicrobial. The synthesized truncated lead peptide displayed weak antimicrobial activity. However, by following a systematic amino acid replacement strategy, a modified peptide was developed that retained the potency of the original peptide. The optimized peptide StAMP-9 displayed bactericidal activity, with minimal inhibitory concentrations of 7.8 µg/mL against Staphylococcus aureus and 3.9 µg/mL against Escherichia coli, and no cytotoxic effects against mammalian cells. Preliminary experiments indicate the bacterial membranes as immediate and primary targets.
Bioinformatics and Molecular Insights to Anti-Metastasis Activity of Triethylene Glycol Derivatives
Authors: Malik Vidhi , Garg Sukant , Afzal Sajal , Dhanjal Jaspreet Kaur , Yun Chae-Ok , Kaul Sunil C. , Sundar Durai , Wadhwa Renu
Published: July 2020

The anti-metastatic and anti-angiogenic activities of triethylene glycol derivatives have been reported. In this study, we investigated their molecular mechanism(s) using bioinformatics and experimental tools. By molecular dynamics analysis, we found that (i) triethylene glycol dimethacrylate (TD-10) and tetraethylene glycol dimethacrylate (TD-11) can act as inhibitors of the catalytic domain of matrix metalloproteinases (MMP-2, MMP-7 and MMP-9) by binding to the S1’ pocket of MMP-2 and MMP-9 and the catalytic Zn ion binding site of MMP-7, and that (ii) TD-11 can cause local disruption of the secondary structure of vascular endothelial growth factor A (VEGFA) dimer and exhibit stable interaction at the binding interface of VEGFA receptor R1 complex. Cell-culture-based in vitro experiments showed anti-metastatic phenotypes as seen in migration and invasion assays in cancer cells by both TD-10 and TD-11. Underlying biochemical evidence revealed downregulation of VEGF and MMPs at the protein level; MMP-9 was also downregulated at the transcriptional level. By molecular analyses, we demonstrate that TD-10 and TD-11 target stress chaperone mortalin at the transcription and translational level, yielding decreased expression of vimentin, fibronectin and hnRNP-K, and increase in extracellular matrix (ECM) proteins (collagen IV and E-cadherin) endorsing reversal of epithelial–mesenchymal transition (EMT) signaling.
Aquilariae Lignum Methylene Chloride Fraction Attenuates IL-1β-Driven Neuroinflammation in BV2 Microglial Cells
Authors: Lee Jin-Seok , Jeon Yoo-Jin , Kang Ji-Yun , Lee Sam-Keun , Lee Hwa-Dong , Son Chang-Gue
Published: July 2020

Microglial hyperactivation and neuroinflammation are known to induce neuronal death, which is one of the main causes of neurodegenerative disorders. We previously found that Aquilariae Lignum extract attenuated both neuronal excitotoxicity and neuroinflammation in vivo and in vitro. For further analysis, we extracted the methylene chloride fraction of Aquilariae Lignum to determine the bioactive compounds. In this study, we investigated the anti-neuroinflammatory effects and underlying mechanisms of the Aquilariae Lignum fraction (ALF) using lipopolysaccharide (LPS)-stimulated BV2 microglial cells. BV2 cells were pretreated with ALF (0.5, 1, and 2.5 μg/mL) before treatment with LPS (1 μg/mL). Pretreatment with ALF significantly attenuated the LPS-induced overproductions of nitric oxide (NO), cyclooxygenase-2 (COX-2), prostaglandin E2 (PGE2), and interleukin (IL)-1β. These anti-inflammatory effects were supported by ALF-mediated modulation of the nuclear factor-kappa B (NF-κB) pathway. Furthermore, ALF exerted strong anti-inflammasome effects, as shown by IL-1β-specific inhibitory activity, but not activity against tumor necrosis factor (TNF)-α, along with inhibition of caspase-1 activity and NACHT, LRR, and PYD domain-containing protein 3 (NLRP3)-related molecules. These results indicate the potent anti-neuroinflammatory activity of ALF and that its underlying mechanism may involve the regulation of NLRP3 inflammasome-derived neuroinflammation in microglial cells.
Cold Press Pomegranate Seed Oil Attenuates Dietary-Obesity Induced Hepatic Steatosis and Fibrosis through Antioxidant and Mitochondrial Pathways in Obese Mice
Authors: Raffaele Marco , Licari Maria , Amin Sherif , Alex Ragin , Shen Hsin-hsueh , Singh Shailendra P. , Vanella Luca , Rezzani Rita , Bonomini Francesca , Peterson Stephen J. , Stec David E. , Abraham Nader G.
Published: July 2020

Aim: Obesity is associated with metabolic syndrome, hypertension, dyslipidemia, nonalcoholic fatty liver disease (NAFLD), and type 2 diabetes. In this study, we investigated whether the dietary supplementation of pomegranate seed oil (PSO) exerted a protective effect on liver lipid uptake, fibrosis, and mitochondrial function in a mouse model of obesity and insulin resistance. Method: In this in vivo study, eight-week-old C57BL/6J male mice were fed with a high fat diet (HFD) for 24 weeks and then were divided into three groups as follows: group (1) Lean; group (n = 6) (2) HF diet; group (n = 6) (3) HF diet treated with PSO (40 mL/kg food) (n = 6) for eight additional weeks starting at 24 weeks. Physiological parameters, lipid droplet accumulation, inflammatory biomarkers, antioxidant biomarkers, mitochondrial biogenesis, insulin sensitivity, and hepatic fibrosis were determined to examine whether PSO intervention prevents obesity-associated metabolic syndrome. Results: The PSO group displayed an increase in oxygen consumption, as well as a decrease in fasting glucose and blood pressure (p < 0.05) when compared to the HFD-fed mice group. PSO increased both the activity and expression of hepatic HO-1, downregulated inflammatory adipokines, and decreased hepatic fibrosis. PSO increased the levels of thermogenic genes, mitochondrial signaling, and lipid metabolism through increases in Mfn2, OPA-1, PRDM 16, and PGC1α. Furthermore, PSO upregulated obesity-mediated hepatic insulin receptor phosphorylation Tyr-972, p-IRB tyr1146, and pAMPK, thereby decreasing insulin resistance. Conclusions: These results indicated that PSO decreased obesity-mediated insulin resistance and the progression of hepatic fibrosis through an improved liver signaling, as manifested by increased insulin receptor phosphorylation and thermogenic genes. Furthermore, our findings indicate a potential therapeutic role for PSO in the prevention of obesity-associated NAFLD, NASH, and other metabolic disorders.
Identification and Validation of Circulating Micrornas as Prognostic Biomarkers in Pancreatic Ductal Adenocarcinoma Patients Undergoing Surgical Resection
Authors: Gablo Natalia , Trachtova Karolina , Prochazka Vladimir , Hlavsa Jan , Grolich Tomas , Kiss Igor , Srovnal Josef , Rehulkova Alona , Lovecek Martin , Skalicky Pavel , Berindan-Neagoe Ioana , Kala Zdenek , Slaby Ondrej
Published: July 2020

Pancreatic ductal adenocarcinoma (PDAC) is one of the most lethal and aggressive cancers with a less than 6% five-year survival rate. Circulating microRNAs (miRNAs) are emerging as a useful tool for non-invasive diagnosis and prognosis estimation in the various cancer types, including PDAC. Our study aimed to evaluate whether miRNAs in the pre-operative blood plasma specimen have the potential to predict the prognosis of PDAC patients. In total, 112 PDAC patients planned for surgical resection were enrolled in our prospective study. To identify prognostic miRNAs, we used small RNA sequencing in 24 plasma samples of PDAC patients with poor prognosis (overall survival (OS) < 16 months) and 24 plasma samples of PDAC patients with a good prognosis (OS > 20 months). qPCR validation of selected miRNA candidates was performed in the independent cohort of PDAC patients (n = 64). In the discovery phase of the study, we identified 44 miRNAs with significantly different levels in the plasma samples of the group of good and poor prognosis patients. Among these miRNAs, 23 showed lower levels, and 21 showed higher levels in plasma specimens from PDAC patients with poor prognosis. Eleven miRNAs were selected for the validation, but only miR-99a-5p and miR-365a-3p were confirmed to have significantly lower levels and miR-200c-3p higher levels in plasma samples of poor prognosis cases. Using the combination of these 3-miRNA levels, we were able to identify the patients with poor prognosis with sensitivity 85% and specificity 80% (Area Under the Curve = 0.890). Overall, 3-miRNA prognostic score associated with OS was identified in the pre-operative blood plasma samples of PDAC patients undergoing surgical resection. Following further independent validations, the detection of these miRNA may enable identification of PDAC patients who have no survival benefit from the surgical treatment, which is associated with the high morbidity rates.
Models Contribution to the Understanding of Sarcoidosis Pathogenesis: “Are There Good Models of Sarcoidosis?”
Authors: Besnard Valérie , Jeny Florence
Published: July 2020

Sarcoidosis is a systemic, granulomatous, and noninfectious disease of unknown etiology. The clinical heterogeneity of the disease (targeted tissue(s), course of the disease, and therapy response) supports the idea that a multiplicity of trigger antigens may be involved. The pathogenesis of sarcoidosis is not yet completely understood, although in recent years, considerable efforts were put to develop novel experimental research models of sarcoidosis. In particular, sarcoidosis patient cells were used within in vitro 3D models to study their characteristics compared to control patients. Likewise, a series of transgenic mouse models were developed to highlight the role of particular signaling pathways in granuloma formation and persistence. The purpose of this review is to put in perspective the contributions of the most recent models in the understanding of sarcoidosis.