bluuebunny/crossref_metadata_2025 huggingface dataset:
Survival Prediction of Patients Treated With Immune Checkpoint Inhibitors via KRAS/TP53/EGFR-Single Gene Mutation
Authors: Liu Shui , Geng Shuai , Shi Ning , Zhang Lili , Xue Wenxin , Li Yiwen , Jiang Kai
Published: March 2022

Background: Immune checkpoint inhibitors (ICIs) have become an effective treatment option for cancer. KRAS, EGFR and TP53 are common mutated oncogenes in cancer whose single gene status may predict the therapeutic effect of clinical ICIs. In this efficacy evaluation, we aimed to clarify whether the single gene mutation status of KRAS, EGFR or TP53 affects the survival benefits of ICIs in cancer patients.

Methods: We used PubMed, Cochrane Library, web of science, and clinical trials Gov database to retrieve qualified documents, the time was up to January 2022. Hazard ratios (HRS) and 95% confidence intervals (CIs) were used to determine the single gene mutation status and no progression of KRAS, EGFR or TP53.

Results: A total of 19 studies included 7029 cancer patients treated with ICIs. The results showed that KRAS, EGFR or TP53 single gene mutation could significantly improve PFS and OS in patients receiving ICIs, but the degree of improvement was different. The risk of prolongation of PFS (HR = 1.48, 95% CI = 1.19-1.85, p = 0.0004) and OS (HR = 1.68, 95% CI = 1.36-2.07, p < 0.00001) caused by TP53 single gene mutation was relatively high, the risk ratio of prolongation of PFS (HR = 1.38, 95% CI = 1.21-1.57, p < 0.00001) and OS (HR = 1.56, 95% CI = 1.20-2.04, p = 0.001) caused by EGFR single gene mutation was the second, the risk ratio of prolongation of PFS (HR = 1.33, 95% CI = 1.12-1.57, p = 0.001) and OS (HR = 1.39, 95% CI = 1.18-1.63, p < 0.00001) caused by KRAS single gene mutation was relatively low, and the results were significantly different.

Conclusion: In cancer patients, KRAS, EGFR or TP53 single gene status is correlated with the benefits of immunotherapy PFS and OS, which suggests that gene sequencing should be carried out in time in the process of clinical treatment to determine the gene mutation of patients and better predict the clinical treatment effect of ICIs.
Ultra Fast Switching of DFLC Based Dynamic Metasurfaces
Authors: Sakhare P. A. , Dontabhaktuni Jayasri
Published: March 2022

Dielectric metasurfaces give rise to very interesting optical and photonic properties such as Huygens lens, absolute transmission and absorption, directional scattering, etc. Liquid crystal based dynamic metasurfaces are being increasingly explored due to their excellent tunability of polarization, phase and amplitude modulations, enabling applications in spatial light modulators (SLM’s), holography, AR and VR and flat optics. We investigate the effect of geometry of dielectric microstructures on electromagnetic response and switching of Dual frequency liquid crystal based metasurfaces in the mid-IR range of frequencies. Scattering response, near-field profiles and far-field radiation show significant dependence on the alignment and geometry of the microstructures. At selected frequencies switching between different polarization directions and variable phase modulations are observed simultaneously. Response times calculated theoretically show switching times of the order of milliseconds paving way for ultrafast multifunctional active metasurfaces.
Effects of Different Ion Erosion on the Hydration Mechanism and Microstructure of Nano-Clay Single-Liquid Grouting Material
Authors: Hong Zi-jie , Zuo Jian-ping
Published: March 2022

The effect of nano-clay (NC) on hydration and microstructure of grouting materials was investigated under different erosion conditions. Six different contents of NC specimens were prepared. This study investigated the effect of NC on the mechanical properties and microstructure of grouting materials under chloride ion erosion and standard curing conditions (SCCs), using ultrasonic pulse velocity (UPV), uniaxial compressive strength (UCS), Fourier transform infrared spectroscopy (FT-IR), and scanning electron microscopy (SEM). The experimental results demonstrated that the nano-core effect can promote the formation of the core reaction of grouting materials and accelerate the hydration process. With the NC increase, the hydration products of ettringite (AFt) and calcium silicate hydrate (C-S-H) also increases. Under chloride ion erosion, it was obtained that when NC was less than 2%, the UCS and UPV were improved. As the NC content is 2%, the hydration reaction is the most appropriate. However, under high humidity conditions, as the NC content is 1%, the amount and density of AFt increased and the formation of C-S-H gel enhanced. The hydration reaction inhibited, and the mechanical properties of grouting materials decreased when NC was further increased. It indicated that the complex underground engineering environment affected the hydration mechanism of nano-grouting materials.
Hemodynamic and Structural Comparison of Human Fetal Heart Development Between Normally Growing and Hypoplastic Left Heart Syndrome-Diagnosed Hearts
Authors: Salman Huseyin Enes , Kamal Reema Yousef , Hijazi Ziyad M. , Yalcin Huseyin Cagatay
Published: March 2022

Congenital heart defects (CHDs) affect a wide range of societies with an incidence rate of 1.0–1.2%. These defects initiate at the early developmental stage and result in critical health disorders. Although genetic factors play a role in the formation of CHDs, the occurrence of cases in families with no history of CHDs suggests that mechanobiological forces may also play a role in the initiation and progression of CHDs. Hypoplastic left heart syndrome (HLHS) is a critical CHD, which is responsible for 25–40% of all prenatal cardiac deaths. The comparison of healthy and HLHS hearts helps in understanding the main hemodynamic differences related to HLHS. Echocardiography is the most common imaging modality utilized for fetal cardiac assessment. In this study, we utilized echocardiographic images to compare healthy and HLHS human fetal hearts for determining the differences in terms of heart chamber dimensions, valvular flow rates, and hemodynamics. The cross-sectional areas of chamber dimensions are determined from 2D b-mode ultrasound images. Valvular flow rates are measured via Doppler echocardiography, and hemodynamic quantifications are performed with the use of computational fluid dynamics (CFD) simulations. The obtained results indicate that cross-sectional areas of the left and right sides of the heart are similar for healthy fetuses during gestational development. The left side of HLHS heart is underdeveloped, and as a result, the hemodynamic parameters such as flow velocity, pressure, and wall shear stress (WSS) are significantly altered compared to those of healthy hearts.
Liquid Anaerobic Digestate as Sole Nutrient Source in Soilless Horticulture—Or Spiked With Mineral Nutrients for Improved Plant Growth
Authors: Weimers Kristina , Bergstrand Karl-Johan , Hultberg Malin , Asp Håkan
Published: March 2022

Digestate from biogas production high in plant-available macro- and micro-nutrients could replace mineral fertilizer in protected (soilless) horticulture. Previous uses of digestate have shown that low concentrations of plant-available phosphorus (P) and sulfur (S) may be limiting factors for growth when using digestate as the sole fertilizer. In this study, digestate collected from a municipal biogas plant in Sweden was nitrified in a moving-bed biofilm reactor prior to its use as fertilizer. A greenhouse pot trial with pak choi grown in peat-based growing medium was established to assess the (i) macro- and micro-nutrient availability in the digestate, with particular focus on P and S and (ii) the effect of amending the digestate solution with nutrients considered to be lacking [P, S, magnesium (Mg), manganese (Mn), boron (B), and molybdenum (Mo)]. The results showed that plants fertilized with raw digestate suffered from S and B deficiency and early P deficiency. Supplementing the digestate with nutrients originating from mineral salts resulted in sufficient plant tissue concentrations of all elements except S. The marketable yield was similar to that achieved using standard mineral fertilizer and the dry matter yield was 17% higher. In the light of the present results, the use of nitrified digestate in soilless plant production seems like a fruitful way forward to recycle organic nutrients from waste streams. In the case where a strict organic protocol is not needed, amendment with inorganic nutrients may be a way to increase the utilization of organically derived nutrients.
Multidimensional Role of Silicon to Activate Resilient Plant Growth and to Mitigate Abiotic Stress
Authors: Mir Rakeeb Ahmad , Bhat Basharat Ahmad , Yousuf Henan , Islam Sheikh Tajamul , Raza Ali , Rizvi Masood Ahmad , Charagh Sidra , Albaqami Mohammed , Sofi Parvaze A. , Zargar Sajad Majeed
Published: March 2022

Sustainable agricultural production is critically antagonistic by fluctuating unfavorable environmental conditions. The introduction of mineral elements emerged as the most exciting and magical aspect, apart from the novel intervention of traditional and applied strategies to defend the abiotic stress conditions. The silicon (Si) has ameliorating impacts by regulating diverse functionalities on enhancing the growth and development of crop plants. Si is categorized as a non-essential element since crop plants accumulate less during normal environmental conditions. Studies on the application of Si in plants highlight the beneficial role of Si during extreme stressful conditions through modulation of several metabolites during abiotic stress conditions. Phytohormones are primary plant metabolites positively regulated by Si during abiotic stress conditions. Phytohormones play a pivotal role in crop plants’ broad-spectrum biochemical and physiological aspects during normal and extreme environmental conditions. Frontline phytohormones include auxin, cytokinin, ethylene, gibberellin, salicylic acid, abscisic acid, brassinosteroids, and jasmonic acid. These phytohormones are internally correlated with Si in regulating abiotic stress tolerance mechanisms. This review explores insights into the role of Si in enhancing the phytohormone metabolism and its role in maintaining the physiological and biochemical well-being of crop plants during diverse abiotic stresses. Moreover, in-depth information about Si’s pivotal role in inducing abiotic stress tolerance in crop plants through metabolic and molecular modulations is elaborated. Furthermore, the potential of various high throughput technologies has also been discussed in improving Si-induced multiple stress tolerance. In addition, a special emphasis is engrossed in the role of Si in achieving sustainable agricultural growth and global food security.
Programmed Cell Death May Be Involved in the Seedless Phenotype Formation of Oil Palm
Authors: Htwe Yin Min , Shi Peng , Zhang Dapeng , Li Zhiying , Xiao Yong , Yang Yaodong , Lei Xintao , Wang Yong
Published: March 2022

Oil palm (Elaeis guineensis Jacq.) is a well-known vegetable oil-yielding crop. Seedlessness is one of the most prominent traits in oil palm due to its low processing costs and high oil content. Nevertheless, an extensive study on molecular mechanisms regulating seedless phenotype formation in oil palm is very limited so far. In this study, stigma, style, and ovary from seedless and seeded (Tenera and Pisifera) oil palm trees were used to investigate the possible mechanism. Results showed that non-pollination resulted in no fruits, and self- and cross-pollinations resulted in seedless fruits, while boron treatment had no effect on seedless phenotype formation, implying that seedless trees have incomplete self and outcrossing incompatibility. Furthermore, the transcriptome data analysis highlighted eight programmed cell death (PCD) genes and three groups of PCD-related genes: 4-coumarate-CoA ligase (4CL), S-RNase, and MADS-box. The majority of these genes were significantly up-regulated in the stigma and style of Seedless palm trees compared to Tenera and Pisifera. In addition, the co-expression network analysis confirmed the significant correlation among these genes. Moreover, two simple sequence repeats (SSR) markers (S41 and S44) were developed to identify the seedless phenotype. The up-regulation of 4CL and MADS-box TFs activated the expression of PCD genes; on the other hand, S-RNase resulted in pollen tube RNA degradation and triggered PCD. While the link between PCD and seedless phenotype formation in oil palm has not been extensively studied to date, these findings suggest a role of PCD in pollen tube lethality, leading to double fertilization failure and the seedless phenotype.
Studies on Viroid Shed Light on the Role of RNA Three-Dimensional Structural Motifs in RNA Trafficking in Plants
Authors: Ma Junfei , Wang Ying
Published: March 2022

RNAs play essential roles in various biological processes. Mounting evidence has demonstrated that RNA subcellular localization and intercellular/systemic trafficking govern their functions in coordinating plant growth at the organismal level. While numerous types of RNAs (i.e., mRNAs, small RNAs, rRNAs, tRNAs, and long noncoding RNAs) have been found to traffic in a non-cell-autonomous fashion within plants, the underlying regulatory mechanism remains unclear. Viroids are single-stranded circular noncoding RNAs, which entirely rely on their RNA motifs to exploit cellular machinery for organelle entry and exit, cell-to-cell movement through plasmodesmata, and systemic trafficking. Viroids represent an excellent model to dissect the role of RNA three-dimensional (3D) structural motifs in regulating RNA movement. Nearly two decades of studies have found multiple RNA 3D motifs responsible for viroid nuclear import as well as trafficking across diverse cellular boundaries in plants. These RNA 3D motifs function as “keys” to unlock cellular and subcellular barriers and guide RNA movement within a cell or between cells. Here, we summarize the key findings along this line of research with implications for future studies on RNA trafficking in plants.
Chemical Composition of Lipophilic Compounds From Rice (Oryza sativa) Straw: An Attractive Feedstock for Obtaining Valuable Phytochemicals
Authors: Rosado Mario J. , Marques Gisela , Rencoret Jorge , Gutiérrez Ana , del Río José C.
Published: March 2022

Rice (Oryza sativa L.) straw is a highly abundant, widely available, and low cost agricultural waste that can be used as a source to extract valuable phytochemicals of industrial interest. Hence, in the present work, the chemical composition of the lipophilic compounds present in rice straw was thoroughly characterized by gas chromatography and mass spectrometry using medium-length high-temperature capillary columns, which allowed the identification of a wide range of lipophilic compounds, from low molecular weight fatty acids to high molecular weight sterols esters, sterol glucosides, or triglycerides in the same chromatogram. The most abundant lipophilic compounds in rice straw were fatty acids, which accounted for up to 6,400 mg/kg (41.0% of all identified compounds), followed by free sterols (1,600 mg/kg; 10.2%), sterol glucosides (1,380 mg/kg; 8.8%), fatty alcohols (1,150 mg/kg; 7.4%), and triglycerides (1,140 mg/kg; 7.3%), along with lower amounts of high molecular weight wax esters (900 mg/kg; 5.8%), steroid ketones (900 mg/kg; 5.8%), monoglycerides (600 mg/kg; 3.8%), alkanes (400 mg/kg; 2.6%), diglycerides (380 mg/kg; 2.4%), sterol esters (380 mg/kg; 2.4%), tocopherols (340 mg/kg; 2.2%), and steroid hydrocarbons (60 mg/kg; 0.4%). This information is of great use for the valorization of rice straw to obtain valuable lipophilic compounds of interest for the nutraceutical, pharmaceutical, cosmetic, and chemical industries. Moreover, this knowledge is also useful for other industrial uses of rice straw, as in pulp and papermaking, since some lipophilic compounds are at the origin of the so-called pitch deposits during pulping.
An Empirical Approach to Analyzing the Effects of Stress on Individual Creativity in Business Problem-Solving: Emphasis on the Electrocardiogram, Electroencephalogram Methodology
Authors: Lee Jungwoo , Kim Cheong , Lee Kun Chang
Published: March 2022

In this study, experiments were conducted on 30 subjects by means of electrocardiogram (ECG) and electroencephalogram (EEG) methodologies as well as a money game to examine the effects of stress on creativity in business problem-solving. The study explained the relationship between creativity and human physiological response using the biopsychosocial model of challenge and threat. The subjects were asked to perform a cognitive mapping task. Based on the brain wave theory, we identified the types of brain waves and locations of brain activities that occurred during the creative problem-solving process in a business environment and studied the effects of stress on creativity. The results of the experiments showed significant differences in creativity in business problem-solving depending on whether or not stress was triggered. Differences were found in the time domain (SDNN, RMSSD) and frequency domain (HF, LF/HF ratio) of heart rates, a physiological stress indicator, between the stress group and the no-stress group. A brain wave analysis confirmed that alpha waves increased in the frontal lobe of the brain during creative business problem-solving but decreased when the subjects were under stress, during which beta waves in the brain increased. This study seeks to examine creativity in business problem-solving by studying the effects of stress on human physiological response and cognitive functions in the hope of providing a new and objective interpretation of existing research results.
Personality Traits and Cyberbullying Perpetration Among Chinese University Students: The Moderating Role of Internet Self-Efficacy and Gender
Authors: Xu Wei , Zheng Shujie
Published: March 2022

Cyberbullying is a serious global problem that affects many teenagers and university students. Recent studies have explored the relationship between personality traits and cyberbullying, but the mechanism needs further research. This paper examines the impact of personality traits on cyberbullying perpetration of Chinese university students and the moderating role of Internet self-efficacy (ISE) and gender. By random cluster sampling, 549 university students (45.7% boys) participated in filling out the self-report questionnaires. The results revealed: (1) conscientiousness, agreeableness, and openness were significantly negatively correlated with cyberbullying perpetration. (2) The stratified regression showed that ISE moderated the relation between agreeableness and cyberbullying. Gender moderated the relationship between agreeableness and cyberbullying, and openness and cyberbullying.
Dispositional Mindfulness and Post-traumatic Stress Symptoms in Emergency Nurses: Multiple Mediating Roles of Coping Styles and Emotional Exhaustion
Authors: Yuan Yuan , Wang Zonghua , Shao Yanxia , Xu Xia , Lu Fang , Xie Fei , Sun Wei
Published: March 2022

Objective

To explore the relationships between dispositional mindfulness (DM) and their post-traumatic stress symptoms (PTSS) of emergency nurses, and the mediating effects of coping styles and emotional exhaustion (EE).

Methods

A cross-sectional survey study was conducted to collect data on DM, coping styles, EE, and PTSS among 571 emergency nurses from 20 hospitals in Chongqing, China. Correlation and structural equation models (SEMs) were used to evaluate the relationship among variables.

Results

Emergency nurses with lower dispositional mindfulness, higher emotional exhaustion and preference for negative coping (NC) revealed more PTSS. The effect of NC on PTSS was partially mediated by emotional exhaustion. Negative coping and emotional exhaustion played concurrent and sequential mediating roles between dispositional mindfulness and PTSS.

Conclusion

This study has made a significant contribution to existing literature. It was suggested to develop interventions aimed at enhancing mindfulness, reducing negative coping strategies, and alleviating emotional exhaustion, which may be effective at reducing or alleviating post-traumatic stress symptoms of emergency nurses.
How Classy Servant Leader at Workplace? Linking Servant Leadership and Task Performance During the COVID-19 Crisis: A Moderation and Mediation Approach
Authors: Zada Muhammad , Zada Shagufta , Ali Mudassar , Jun Zhang Yong , Contreras-Barraza Nicolás , Castillo Dante
Published: March 2022

The COVID-19 pandemic has caused a record global crisis, particularly and extremely, for the service sectors. Due to extensive security measures, many service sector employees have to work remotely to maintain services. Drawing upon the conservation of resources theory, this research investigates the impact of servant leadership on the task performance of employees in virtual working environments during the COVID-19 crisis. Our theoretical model was tested using data collected from 335 individual employees in the education sector of Pakistan. SPSS version 26.0 was applied to find the hypothesized relationship between the study variables. To find the indirect mediating effect, we applied Model 4; for moderation, we applied Model 1; and for the moderation and mediation effect, we applied Model 7 of the Process Macro model of Hayes. The results of the study revealed that servant leadership is positively related to task performance in a virtual environment during crises. Furthermore, psychological empowerment partially mediates the relationship between servant leadership and task performance. Perceived supervisor support positively moderates the relationship between servant leadership and task performance. Moreover, the indirect effect of servant leadership on task performance via psychological empowerment is moderated by perceived supervisor support. The results provided guidance to the educational sector on how to lead effectively in times of crisis when service sector employees work predominantly in virtual environments. The theoretical and practical implications of these findings are discussed.
Affective Valence and Enjoyment in High- and Moderate-High Intensity Interval Exercise. The Tromsø Exercise Enjoyment Study
Authors: Hammer Tord Markussen , Pedersen Sigurd , Pettersen Svein Arne , Rognmo Kamilla , Sagelv Edvard H.
Published: March 2022

Introduction

Exercise at high intensity may cause lower affective responses toward exercise compared with moderate intensity exercise. We aimed to elucidate affective valence and enjoyment in high- and moderate-high interval exercise.

Methods

Twenty recreationally active participants (9 females, 11 males, age range: 20–51 years) underwent three different treadmill running exercise sessions per week over a 3-week period, in randomized order; (1) CE70: 45 min continuous exercise at 70% of heart rate maximum (HRmax), (2) INT80: 4 × 4 min intervals at 80% of HRmax, (3) INT90: 4 × 4 min intervals at 90% of HRmax. Pre-tests included graded submaximal steady state intensities and a test to exhaustion for determining peak oxygen uptake and HRmax. Affective valence (pleasure/displeasure) was measured before, during and after the sessions using the Feeling Scale (FS). Enjoyment was assessed before and after the sessions applying the Physical Activity Enjoyment Scale (PACES) and during the sessions using the Exercise Enjoyment Scale (EES).

Results

The participants felt lower pleasure (between-sessions effect:p= 0.02,pη2: 0.13) during INT90 sessions (FS: 1.08, 95% CI: 0.35–1.92) compared with INT80 (FS: 2.35, 95% CI: 1.62–3.08,p= 0.052) and CE70 sessions (FS: 2.45, 95% CI: 1.72–3.18,p= 0.03), with no differences between INT80 and CE70 sessions (p= 1.00). There were higher enjoyment after INT80 sessions (PACES: 101.5, 95% CI: 95.7–107.3) versus CE70 sessions (PACES: 91.3 95% CI: 85.5–97.1,p= 0.046), and no differences between INT90 (PACES: 98.2, 95% CI: 92.4–103.4) and CE70 (p= 0.29) or INT80 (p= 1.00). For enjoyment during exercise, CE70 were perceived more enjoyable, and INT80 and INT90 less enjoyable in week 2 (EES: week x session:p= 0.01,pη2: 0.11; CE70: 4.3, 95% CI: 3.6–4.9, INT80: 4.6, 95% CI: 3.9–5.2, INT90: 4.0, 95% CI: 3.4–4.7) and 3 (EES: CE70: 4.2, 95% CI: 3.7–4.8, INT80: 4.8, 95% CI: 4.2–5.3, INT90: 4.3, 95% CI: 3.8–4.9) than in week 1 (EES: CE70: 3.5, 95% CI: 3.0–4.0, INT80: 5.0, 95% CI: 4.5–5.5, INT90: 4.5, 95% CI: 4.0–5.0).

Conclusion

The negative affective consequences associated with high intensity interval exercise can be alleviated by keeping the intensity at or around 80% of HRmaxwhile preserving the beneficial enjoyment responses associated with interval exercise.
Empirical Study on the Relationship Between Vacation Schedule and Seafarers’ Fatigue in Chinese Seafarer Population
Authors: An Ji , Gao Wenting , Liu Runze , Liu Ziqi
Published: March 2022

Background

Fatigue is an important factor for the safety of ships. In order to alleviate fatigue of the seafarers, the STCW Convention (International Convention on Standards of Training, Certification, and Watchkeeping for Seafarers) has made many regulations on the working time of seafarers. At present, if a crew member takes only one day off at home before returning to work on the ship, the working time on the ship must be re-calculated again. If the time spent at home is not sufficient to allow the crew to recover, the regulations of only stipulating the working time, not stipulating the home vacation time, cannot guarantee the crew’s fatigue been well controlled. The aim of present study is to explore the relationship between vacation schedule and fatigue of the seafarers.

Methods

In present study, a simplified stress scale developed by the Ministry of Labor of Japan has been used as a measurement tool. The method of stratified sampling was adopted. Data collection mainly came from domestic ocean-going seafarers (n = 165). Analysis was conducted using the Cross (chi-square) analysis and hierarchical multiple regression analysis methods.

Results

We found that there was no difference between crew members of different positions in terms of average vacation time and on-board service time (p > 0.05). The length of last vacation time and this service time for seafarers of different positions showed obvious differences (p < 0.01). The rank has a significant effect on the length of the last vacation (χ2 = 101.560, p = 0.000 < 0.01) and the length of this service time (χ2 = 75.624, p = 0.000 < 0.01). Also, the results showed that there was a significant negative correlation between the duration of vacation and overall fatigue (t = –7.160, p = 0.000 < 0.01), while there was a significant positive correlation between the length of service time on board and overall fatigue (t = 3.474, p = 0.001 < 0.01).

Conclusion

The results indicated that a reasonable vacation schedule was crucial for the relief of the seafarers’ fatigue, and also played a positive role in the state of working on the ship again.
A Multi-level Remedial Teaching Design Based on Cognitive Diagnostic Assessment: Taking the Electromagnetic Induction as an Example
Authors: Huang Rui , Liu Zengze , Zi Defu , Huang Qinmei , Pan Sudong
Published: March 2022

Multi-level teaching has been proven to be more effective than a one-size-fits-all learning approach. This study aimed to develop and implement a multi-level remedial teaching scheme in various high school classes containing students of a wide range of learning levels and to determine its effect of their learning. The deterministic inputs noisy and gate model of cognitive diagnosis theory was used to classify students at multiple levels according to their knowledge and desired learning outcomes. A total of 680 senior high school students from central provinces in China participated in the initial cognitive diagnostic test, and 1,615 high school sophomores from seven high schools in China participated in a formal cognitive diagnosis test. Thirty-six high school students from Southwestern China participated in the think-aloud protocols, and 258 seniors from three high schools in southwest China participated in the remedial teaching experiment. Through an analysis of students’ think-aloud protocols, cognitive errors of students at all levels were determined, and multi-level remedial teaching programs were designed to address these common cognitive errors. The remedial teaching programs were then implemented in three schools and compared with a control group. The results indicated that the students in the experimental group showed a more significant improvement. In this study, the steps of designing multi-level remedial teaching include assessment, classification, and preparing a teaching scheme, which are feasible and can have remarkable teaching effects. This process can be used for reference by teachers of various subjects.
Factors Affecting Middle School Teachers’ Attitudes Toward the Inclusion of Students With Disabilities
Authors: Aldosari Mubarak S.
Published: March 2022

Teachers’ positive attitudes are an essential element for the successful inclusion of students who have disabilities in schools with their peers who do not have disabilities. The current quantitative study examines middle school teachers’ attitudes toward the inclusion of students with disabilities in regular schools in Saudi Arabia and the factors that affect their attitudes. Middle school teachers (N = 613) from schools in Riyadh responded to a questionnaire regarding their opinions relative to the integration of students with disabilities. The results indicate that middle school teachers have a neutral attitude toward the inclusion of students with disabilities in regular schools. Moreover, teacher-related factors such as gender, position, and experience do not affect teacher attitudes toward inclusion. In contrast, training in inclusion plays a significant role in middle school teachers’ attitudes toward inclusion of students with disabilities. The implications of the results and suggestions for further research are discussed in the study.
Prevalence and Associated Risk Factors of Suicidal Ideation Among Brazilian Pregnant Women: A Population-Based Study
Authors: Faisal-Cury Alexandre , Oliveira Rodrigues Daniel Maurício , Matijasevich Alicia , Tarpinian Fernanda , Tabb Karen
Published: March 2022

Background

Suicide is a leading cause of death during the perinatal period in high-income countries (HIC). There remains a lack of population-based studies about suicidal ideation (SI) during pregnancy among low and middle income countries (LMIC).

Objective(s)

Using the case of Brazil, we aim to estimate the prevalence of SI during pregnancy and its association with antenatal depression (AD) and sociodemographic factors in a LMIC.

Method

We used data from the Brazilian National Survey (PNS-2019), a population-based study, with a complex and probabilistic sampling method. Of the 27,136 women of reproductive age (15 to 49 years old) who participated in the PNS, a total of 769 women reported being pregnant at the time of the interview. All PNS participants answered the Patient Health Questionnaire-9 (PHQ-9) and a questionnaire with sociodemographic data. SI was defined as any answer to the PHQ-9 item 9 other than 0 (not at all). Logistic regression models were performed to obtain crude and adjusted odds ratios (aOR) and 95% confidence intervals (95% CI) for the association between explanatory variables and SI during pregnancy.

Results

Among 769 women, 33 (3.9%, 95% CI: 3.0–5.1%) reported SI during pregnancy. In the adjusted analysis, higher odds of SI were associated with being 20 to 34 years old (aOR:0.24, 95% CI: 0.08–0.74) or 35 to 49 years old (aOR:0.15; 95% CI: 0.04–0.50), having 9 to 11 years of education (aOR 0.23, 95% CI: 0.61–0.86), acheiving the highest family income category (aOR:0.08, 95% CI: 0.01–0.58), not living in the South/Southeast regions of Brazil (aOR:5.52, 95% CI: 2.36–12.9), and having probable mild AD (aOR:10.5 95% CI: 2.3–47.9) or moderate AD (aOR:241.3, 95% CI: 58.4–996.7).

Conclusion(s)

In Brazil, SI affects almost 4% of pregnant women and is associated with sociodemographic vulnerability. Clinically, women with mild symptoms of depression may also experience SI during pregnancy. These findings are important for designing effective perinatal mental health interventions in LMICs.
Label-Free Determination of PSA and Free PSA Using MA-SERS
Authors: Chaloupková Zuzana , Medříková Zdenka , Král Milan , Šedajová Veronika , Ranc Václav
Published: March 2022

Prostate cancer is one of the compelling types of cancer diagnosed in men. Development of screening analytical methods, which provide fast and reliable results is, thus, demanding. Currently applied methods are usually based on the determination of serum prostate-specific antigen (PSA), where several limitations were identified. However, scientific reports have shown a direct correlation between the percentage of free PSA and prostate volume, and indirect correlation between the unfavorable course of the disease of prostate cancer and the percentage of free PSA in men with elevated PSA levels. Parallel analysis of PSA and free PSA presents an interesting alternative. Here, we present a new analytical method for a parallel analysis of PSA and free PSA in a whole human blood based on MA-SERS. The method is based on magnetic Fe3O4@Ag nanocomposite functionalized using anti-PSA. The method can distinguish between levels of PSA and free PSA within a single analytical run with limits of detection of 0.62 ng/ml for PSA and 0.49 ng/ml for free PSA, respectively.
Mobility Disability and Exercise: Health Outcomes of an Accessible Community-Based Center
Authors: Morgan Kerri A. , Taylor Kelly L. , Walker Carla Wilson , Tucker Susan , Dashner Jessica L. , Hollingsworth Holly
Published: March 2022

Objective

The purpose of this study was to determine how support and guidance provided by trained professionals during a 12-week, community-based transition exercise program, impact health outcomes and continued engagement in physical activity for persons with a mobility disability (PwMD).

Design

A single arm pre-post design was used.

Setting

Accessible community-based health and wellness center.

Participants

The study included 244 PwMD using a mobility device.

Interventions

Participants completed a 12-week transition exercise program provided through an accessible community facility that provided education and support to complete endurance and strength related exercises as well as programming to encourage transition to self-directed engagement in exercise.

Main Outcome Measures

Bodyweight, BMI, pain, perceived exertion, speed, and distance during cardiovascular fitness testing, and strength were measured pre and post exercise program. The number of participants that signed up for a monthly membership after the program was also monitored.

Results

For the total group, average pain reported over previous 30 days decreased significantly (p < 0.01), current daily pain decreased significantly (p < 0.05), perceived exertion at the end of the 9-min endurance test decreased significantly (p < 0.05), and the four upper extremity strength exercises showed large, significant strength gains (p < 0.01) after the program. There was no significant change in bodyweight, BMI, or speed and distance completed during endurance testing. At the completion of the program, 76% of participants enrolled in a monthly membership at the facility with the intentions to continue to exercise regularly.

Conclusions

This study provides evidence that an accessible community-based exercise program, with a transitional component supported by trained professionals, can support the exercise goals of PwMD and improve strength, decrease pain, and may promote regular exercise adoption for PwMD.
Opportunities and Challenges of Virtual Reality in Healthcare – A Domain Experts Inquiry
Authors: Halbig Andreas , Babu Sooraj K. , Gatter Shirin , Latoschik Marc Erich , Brukamp Kirsten , von Mammen Sebastian
Published: March 2022

In recent years, the applications and accessibility of Virtual Reality (VR) for the healthcare sector have continued to grow. However, so far, most VR applications are only relevant in research settings. Information about what healthcare professionals would need to independently integrate VR applications into their daily working routines is missing. The actual needs and concerns of the people who work in the healthcare sector are often disregarded in the development of VR applications, even though they are the ones who are supposed to use them in practice. By means of this study, we systematically involve health professionals in the development process of VR applications. In particular, we conducted an online survey with 102 healthcare professionals based on a video prototype which demonstrates a software platform that allows them to create and utilise VR experiences on their own. For this study, we adapted and extended the Technology Acceptance Model (TAM). The survey focused on the perceived usefulness and the ease of use of such a platform, as well as the attitude and ethical concerns the users might have. The results show a generally positive attitude toward such a software platform. The users can imagine various use cases in different health domains. However, the perceived usefulness is tied to the actual ease of use of the platform and sufficient support for learning and working with the platform. In the discussion, we explain how these results can be generalized to facilitate the integration of VR in healthcare practice.
Storm-Time Evolution of the Equatorial Electron Pitch Angle Distributions in Earth’s Outer Radiation Belt
Authors: Smirnov Artem , Shprits Yuri , Allison Hayley , Aseev Nikita , Drozdov Alexander , Kollmann Peter , Wang Dedong , Saikin Anthony A.
Published: March 2022

In this study we analyze the storm-time evolution of equatorial electron pitch angle distributions (PADs) in the outer radiation belt region using observations from the Magnetic Electron Ion Spectrometer (MagEIS) instrument aboard the Van Allen Probes in 2012–2019. The PADs are approximated using a sum of the first, third and fifth sine harmonics. Different combinations of the respective coefficients refer to the main PAD shapes within the outer radiation belt, namely the pancake, flat-top, butterfly and cap PADs. We conduct a superposed epoch analysis of 129 geomagnetic storms and analyze the PAD evolution for day and night MLT sectors. PAD shapes exhibit a strong energy-dependent response. At energies of tens of keV, the PADs exhibit little variation throughout geomagnetic storms. Cap PADs are mainly observed at energies<300 keV, and their extent in L shrinks with increasing energy. The cap distributions transform into the pancake PADs around the main phase of the storm on the nightside, and then come back to their original shapes during the recovery phase. At higher energies on the dayside, the PADs are mainly pancake during pre-storm conditions and become more anisotropic during the main phase. The quiet-time butterfly PADs can be observed on the nightside at L>5.6. During the main phase, butterfly PADs have stronger 90°-minima and can be observed at lower L-shells (down to L = 5), then transitioning into flat-top PADs at L ∼ 4.5 – 5 and pancake PADs at L<4.5. The resulting PAD coefficients for different energies, locations and storm epochs can be used to test the wave models and physics-based radiation belt codes in terms of pitch angle distributions.
Calf Diarrhea Is Associated With a Shift From Obligated to Facultative Anaerobes and Expansion of Lactate-Producing Bacteria
Authors: Gomez Diego E. , Li Lynna , Goetz Hanne , MacNicol Jennifer , Gamsjaeger Lisa , Renaud David L.
Published: March 2022

Diarrhea is the leading cause of morbidity, mortality and antimicrobial drug use in calves during the first month of age. Alteration in the bacterial communities of the gastrointestinal tract occurs during diarrhea. Diarrheic calves often develop anion gap (AG) acidosis associated with increased concentrations of unmeasured anions including D- and L-lactate. However, studies investigating the association between gut microbiota alterations and the development of acid-base disorders in diarrheic calves are lacking. We investigated the fecal bacterial alterations of calves with diarrhea and its association with changes in blood pH, and AG. Blood and fecal samples from healthy and diarrheic veal calves were taken 7 days after arrival to the farm. The fecal microbiota of healthy and diarrheic calves was assessed by sequencing of 16S ribosomal RNA gene amplicons. Blood gas analysis was completed using an i-Stat analyzer. In healthy calves, higher richness, evenness, and diversity were observed compared to diarrheic calves.Phocaeicola, Bacteroides, Prevotella, Faecalibacterium, Butyricicoccus, Ruminococcaceae and Lachnospiraceae were enriched in healthy compared with diarrheic calves.Enterococcus, Ligilactobacillus, Lactobacilus, Gallibacterium Streptococcus, andEscherichia/Shigellawere enriched in diarrheic calves. In diarrheic calves, an increased abundance of lactate-producing bacteria includingLactobacillus, Streptococcus, Veillonella, LigilactobacillusandOlsenellawas detected. Diarrheic calves had a lower pH and bicarbonate concentration and a higher AG concentration than healthy calves. Together, these results indicate that calf diarrhea is associated with a shift from obligated to facultative anaerobes and expansion of lactate-producing bacteria which are related to acidemia, low bicarbonate and increase AG. Our results highlight the importance of the gastrointestinal microbiota on the clinicopathological changes observed in diarrheic calves.
The Effects of Inorganic Phosphorus Levels on Phosphorus Utilization, Local Bone-Derived Regulators, and BMP/MAPK Pathway in Primary Cultured Osteoblasts of Broiler Chicks
Authors: Li Tingting , Cao Sumei , Liao Xiudong , Shao Yuxin , Zhang Liyang , Lu Lin , Liu Zongping , Luo Xugang
Published: March 2022

Understanding the underlying mechanisms that regulate the bone phosphorus (P) utilization would be helpful for developing feasible strategies to improve utilization efficiency of P in poultry. We aimed to investigate the effects of inorganic P levels on P utilization, local bone-derived regulators and bone morphogenetic protein/mitogen-activated protein kinase (BMP/MAPK) pathway in primary cultured osteoblasts of broiler chicks in order to address whether local bone-derived regulators or BMP/MAPK pathway was involved in regulating the bone P utilization of broilers using an in vitro model. The primary cultured tibial osteoblasts of broiler chicks were randomly divided into one of five treatments with six replicates for each treatment. Then, cells were respectively incubated with 0.0, 0.5, 1.0, 1.5, or 2.0 mmol/L of added P as NaH2PO4 for 24 days. The results showed that as added P levels increased, tibial osteoblastic P retention rate, number and area of mineralized nodules, the mRNA expressions of endopeptidases on the X chromosome (PHEX), dentin matrix protein 1 (DMP1), bone morphogenetic protein 2 (BMP2), and the mRNA and protein expressions of matrix extracellular phosphoglycoprotein (MEPE) increased linearly (p < 0.001) or quadratically (p < 0.04), while extracellular signal-regulated kinase 1 (ERK1) mRNA expression and c-Jun N-terminal kinase 1 (JNK1) phosphorylated level decreased linearly (p < 0.02) or quadratically (p < 0.01). Correlation analyses showed that tibial osteoblastic P retention rate was positively correlated (r = 0.452–0.564, p < 0.03) with MEPE and BMP2 mRNA expressions. Furthermore, both number and area of mineralized nodules were positively correlated (r = 0.414–0.612, p < 0.03) with PHEX, DMP1, MEPE, and BMP2 mRNA expressions but negatively correlated (r = −0.566 to −0.414, p < 0.04) with the ERK1 mRNA expression and JNK1 phosphorylated level. These results suggested that P utilization in primary cultured tibial osteoblasts of broiler chicks might be partly regulated by PHEX, DMP1, MEPE, BMP2, ERK1, and JNK1.
Parametrization of Horizontal and Vertical Transfers for the Street-Network Model MUNICH Using the CFD Model Code_Saturne
Authors: Maison Alice , Flageul Cédric , Carissimo Bertrand , Tuzet Andrée , Sartelet Karine
Published: March 2022

Cities are heterogeneous environments, and pollutant concentrations are often higher in streets compared with in the upper roughness sublayer (urban background) and cannot be represented using chemical-transport models that have a spatial resolution on the order of kilometers. Computational Fluid Dynamics (CFD) models coupled to chemistry/aerosol models may be used to compute the pollutant concentrations at high resolution over limited areas of cities; however, they are too expensive to use over a whole city. Hence, simplified street-network models, such as the Model of Urban Network of Intersecting Canyons and Highways (MUNICH), have been developed. These include the main physico-chemical processes that influence pollutant concentrations: emissions, transport, deposition, chemistry and aerosol dynamics. However, the streets are not discretized precisely, and concentrations are assumed to be homogeneous in each street segment. The complex street micro-meteorology is simplified by considering only the vertical transfer between the street and the upper roughness sublayer as well as the horizontal transfer between the streets. This study presents a new parametrization of a horizontal wind profile and vertical/horizontal transfer coefficients. This was developed based on a flow parametrization in a sparse vegetated canopy and adapted to street canyons using local-scale simulations performed with the CFD model Code_Saturne. CFD simulations were performed in a 2D infinite street canyon, and three streets of various aspect ratios ranging from 0.3 to 1.0 were studied with different incoming wind directions. The quantities of interest (wind speed in the street direction and passive tracer concentration) were spatially averaged in the street to compare with MUNICH. The developed parametrization depends on the street characteristics and wind direction. This effectively represents the average wind profile in a street canyon and the vertical transfer between the street and the urban roughness sublayer for a wide range of street aspect ratios while maintaining a simple formulation.
An Eco-Friendly Synthetic Approach for Copper Nanoclusters and Their Potential in Lead Ions Sensing and Biological Applications
Authors: Saleh Sayed M. , El-Sayed Wael A. , El-Manawaty May A. , Gassoumi Malek , Ali Reham
Published: March 2022

A new preparation route for high-luminescent blue-emission pepsin copper nanoclusters (Pep-CuNCs) is introduced in this work. The synthesized nanoclusters are based on a pepsin molecule, which is a stomach enzyme that works to digest proteins that exist in undigested food. Here, we have developed an eco-friendly technique through microwave-assisted fast synthesis. The resulting copper nanoclusters (CuNCs) exhibit significant selectivity towards Pb(II) ions. The pepsin molecule was utilized as a stabilizer and reducing agent in the production procedure of Pep-CuNCs. The characteristics of the resulting Pep-CuNCs were studied in terms of size, surface modification, and composition using various sophisticated techniques. The CuNCs responded to Pb(II) ions through the fluorescence quenching mechanism of the CuNCs’ fluorescence. Thus, great selectivity of Pep-CuNCs towards Pb(II) ions was observed, allowing sensitive determination of this metal ion at lab-scale and in the environment. The CuNCs have detection limits for Pb(II) in very tenuous concentration at a nanomalar scale (11.54 nM). The resulting Pep-CuNCs were utilized significantly to detect Pb(II) ions in environmental samples. Additionally, the activity of Pep-CuNCs on different human tumor cell lines was investigated. The data for the observed behavior indicate that the Pep-CuNCs displayed their activity against cancer cells in a dose dependent manner against most utilized cancer cell lines.