bluuebunny/crossref_metadata_2025 huggingface dataset:
Targeting the NRF2/HO-1 Antioxidant Pathway in FLT3-ITD-Positive AML Enhances Therapy Efficacy
Authors: Kannan Sankaranarayan , Irwin Mary E. , Herbrich Shelley M. , Cheng Tiewei , Patterson LaNisha L. , Aitken Marisa J. L. , Bhalla Kapil , You M. James , Konopleva Marina , Zweidler-McKay Patrick A. , Chandra Joya
Published: April 2022

Acute myeloid leukemia (AML) is a molecularly heterogenous hematological malignancy, with one of the most common mutations being internal tandem duplication (ITD) of the juxtamembrane domain of the fms-like tyrosine kinase receptor-3 (FLT3). Despite the development of FLT3-directed tyrosine kinase inhibitors (TKI), relapse and resistance are problematic, requiring improved strategies. In both patient samples and cell lines, FLT3-ITD raises levels of reactive oxygen species (ROS) and elicits an antioxidant response which is linked to chemoresistance broadly in AML. NF-E2–related factor 2 (NRF2) is a transcription factor regulating the antioxidant response including heme oxygenase -1 (HO-1), a heat shock protein implicated in AML resistance. Here, we demonstrate that HO-1 is elevated in FLT3-ITD-bearing cells compared to FLT3-wild type (WT). Transient knockdown or inhibitor-based suppression of HO-1 enhances vulnerability to the TKI, quizartinib, in both TKI-resistant and sensitive primary AML and cell line models. NRF2 suppression (genetically or pharmacologically using brusatol) results in decreased HO-1, suggesting that TKI-resistance is dependent on an active NRF2-driven pathway. In AML-patient derived xenograft (PDX) models, brusatol, in combination with daunorubicin, reduces leukemia burden and prolongs survival. Cumulatively, these data encourage further development of brusatol and NRF2 inhibition as components of combination therapy for refractory AML.
Deep Learning-Based Occlusion Handling of Overlapped Plants for Robotic Grasping
Authors: Mohammadzadeh Babr Mohammad , Faghihabdolahi Maryam , Ristić-Durrant Danijela , Michels Kai
Published: April 2022

Instance segmentation of overlapping plants to detect their grasps for possible robotic grasping presents a challenging task due to the need to address the problem of occlusion. We addressed the problem of occlusion using a powerful convolutional neural network for segmenting objects with complex forms and occlusions. The network was trained with a novel dataset named the “occluded plants” dataset, containing real and synthetic images of plant cuttings on flat surfaces with differing degrees of occlusion. The synthetic images were created using the novel framework for synthesizing 2D images by using all plant cutting instances of available real images. In addition to the method for occlusion handling for overlapped plants, we present a novel method for determining the grasps of segmented plant cuttings that is based on conventional image processing. The result of the employed instance segmentation network on our plant dataset shows that it can accurately segment the overlapped plants, and it has a robust performance for different levels of occlusions. The presented plants’ grasp detection method achieved 94% on the rectangle metric which had an angular deviation of 30 degrees and an IoU of 0.50. The achieved results show the viability of our approach on plant species with an irregular shape and provide confidence that the presented method can provide a basis for various applications in the food and agricultural industries.
Laser-Driven Accelerators, Radiations, and Their Applications
Authors: Kim Hyung Taek , Margarone Daniele
Published: April 2022

Particle accelerators and radiation based on radio-frequency (RF) cavities have significantly contributed to the advancement of science and technology in the last century [...]
Investigation of the Power Quality Concerns of Input Current in Single-Phase Frequency Step-Down Converter
Authors: Ashraf Naveed , Abbas Ghulam , Ullah Nasim , Al-Ahmadi Ahmad Aziz , Raza Ali , Farooq Umar , Jamil Mohsin
Published: April 2022

Single-phase direct frequency converters are gaining attraction at the research and academic level as they are rapidly getting space over conventional multistage converters. The converters developed with a rectification and inversion process using a DC-link level are examples of multistage converters with some serious concerns such as an increase in the overall weight, losses, and cost. They also suffer from the low-reliability issue due to the issues involved with DC-link capacitors and problems linked with electromagnetic interference (EMI) caused by high-frequency pulse width modulation (PWM) switching. These problems are addressed with line frequency switching cycloconverters. In these converters, the power quality of the output voltage is improved by governing the amplitude of some selected output pulses or half-cycles. For this purpose, a low-frequency multiple tapping transformer may be used to obtain various voltage levels. However, its use is the main source of increased overall weight, losses, cost, and volume. In transformer eliminated topologies, high-frequency PWM control can be employed to control the magnitude of some selected half cycles of the output voltage. However, this approach may arise some problems related to EMI. In both control techniques, the attention is focused on the power quality of the output voltage only. The concern for the input current is ignored and not yet analyzed. This is one of the critical power quality concerns and requires further investigation. The magnitude control of the output half-cycles causes the variation in the amplitude of some half cycles of the input currents. As a result, all half cycles of the input current become non-symmetric. It generates harmonics that are always of low frequency and cannot be easily filtered out. It results in a high value of the harmonic factor (HF) of the input current. The improvement in the power quality of the output voltages severally degrades the power quality of the input currents. In this research, this problem is investigated with mathematically computed harmonic coefficients with a pulse selective approach. Also, a simple single-phase cycloconverter is introduced to improve the power quality index of the input current. The overall analysis is supported by the results obtained from a Simulink-based environment and a practically constructed prototype.
Analysis of the Diurnal Changes in the Water-Soluble Ion Concentration in Wuhan between 2016 and 2019
Authors: Sun Yingying , Zhao Jinhui , He Chao , Zhang Zhouxiang , Chen Nan , Hu Jiaqi , Liu Huanhuan , Wang Xinlei
Published: April 2022

This study uses online monitoring data from the Hubei Environmental Monitoring Center’s Atmospheric Compound Pollution Automatic Monitoring Station from 2016 to 2019 to analyze the diurnal changes in the concentration of water-soluble ions in particulate matter in Wuhan. During the study period, the concentrations of SO2, NO3−, and SO42− changed significantly, while those of NH4+, NH3, and Ca2+ exhibited minimal differences. SO2 and NO3− showed an annually increasing trend, while NH4+ and SO42− exhibited an annually decreasing trend. The ion concentration was generally higher in the winter and spring and lower in the summer and autumn. The concentration of water-soluble ions was generally higher during the day than at night. However, the “weekend effect” on the change in ion concentrations was substantial and higher during the day than at night. This effect was the strongest for NO3− and the weakest for NH3. These changes in the weekend effect of water-soluble ions in particulate matter clearly revealed the impact of periodic human activities on atmospheric pollution. Taken together, the results of this novel study reveal the diurnal pollution characteristics and “weekend effect” of water-soluble ions with high concentrations in atmospheric aerosols in Wuhan over a four-year period, thus providing relevant insights for Wuhan’s atmospheric mitigation plan.
Application of Time-Lagged Ensemble Quantitative Precipitation Forecasts for Typhoon Morakot (2009) in Taiwan by a Cloud-Resolving Model
Authors: Wang Chung-Chieh , Chen Shin-Hau , Tsuboki Kazuhisa , Huang Shin-Yi , Chang Chih-Sheng
Published: April 2022

Typhoon Morakot (2009) struck Taiwan during 7–9 August and brought extreme rainfall to the southern mountainous regions of the island, with a peak of 2635 mm in 48 h (and most heavy rainfall observed on 8 August), subsequently causing widespread damage. In this study, the time-lagged ensemble method is applied to this unique case to examine its effectiveness and usefulness for the prediction of heavy rainfall events. A series of hindcasts experiments was carried out using a cloud-resolving model with a grid size of 2.5 km at 6-h intervals from 0000 UTC 1 August to 0000 UTC 9 August 2009, with real-time global model products as initial and boundary conditions. It was found that most of the hindcast members initialized at and after 0600 UTC 6 August in the short range (i.e., within 72 h) could capture the magnitude of the rainfall to a reasonable extent, with a peak 48-h amount near or over 2500 mm, under the condition that the track errors were adequately small. With longer lead times, however, the predictability was limited due to larger track errors, and reliable rainfall predictions were impossible for runs with an initial time before or on 5 August. Thus, the probabilities derived from lagged members for extreme rainfall increased dramatically starting from 6 August, when a >80–90% likelihood was indicated that the southern mountainous regions of Taiwan would receive a 48-h rainfall in excess of 1000 mm, and ≥80% in parts of the area to receive over 1500 mm. Therefore, the time evolution of the probability may be useful in decision making and hazard mitigation. The limitations of such a time-lagged system and the potential to make further improvements are also discussed.
Progress in Assays of HMGB1 Levels in Human Plasma—The Potential Prognostic Value in COVID-19
Authors: Štros Michal , Polanská Eva Volfová , Hlaváčová Tereza , Skládal Petr
Published: April 2022

Extracellular HMGB1 protein is known to induce inflammatory responses leading to an inflammatory storm. The outbreak of the Severe Acute Respiratory Syndrome COVID-19 due to the SARS-CoV-2 virus has resulted in a huge health concern worldwide. Recent data revealed that plasma/serum HMGB1 levels of patients suffering from inflammation-mediated disorders—such as COVID-19, cancer, and autoimmune disorders—correlate positively with disease severity and vice versa. A late release of HMGB1 in sepsis suggests the existence of a wide therapeutic window for treating sepsis. Rapid and accurate methods for the detection of HMGB1 levels in plasma/serum are, therefore, of great importance for monitoring the occurrence, treatment success, and survival prediction of patients with inflammation-mediated diseases. In this review, we briefly explain the role of HMGB1 in the cell, and particularly the involvement of extracellular HMGB1 (released from the cells) in inflammation-mediated diseases, with an emphasis on COVID-19. The current assays to measure HMGB1 levels in human plasma—Western blotting, ELISA, EMSA, and a new approach based on electrochemical immunosensors, including some of our preliminary results—are presented and thoroughly discussed.
Blockade on Lin28a Prevents Cognitive Impairment and Disruption of the Blood-Brain Barrier Induced by Chronic Cerebral Hypoperfusion
Authors: Lee Jae-Min , Lee Joo Hee , Kim Youn-Jung
Published: April 2022

Lin28a is an RNA-binding protein involved in the translation and regulation of multiple mRNAs. Lin28a is overexpressed in animal models of brain injury. Similarly, our preliminary study found increased Lin28a expression levels in the animal models four to seven days after chronic cerebral hypoperfusion. Therefore, this current study aimed to evaluate the effects of modulating Lin28a on cognition and brain functions. Vascular dementia (VaD) was induced in 12-week-old male Wistar rats using permanent bilateral common carotid artery occlusion (BCCAO), and these rats were treated with Lin28a siRNA on the fourth and seventh day after BCCAO. From the 42nd day after BCCAO, cognitive behavioral experiments were performed for two weeks. VaD induced by BCCAO resulted in cognitive impairment and microglial activation. Lin28a expression was upregulated after BCCAO. Lin28a siRNA treatment alleviated cognitive impairment and overexpression of GFAP and Iba-1 in the brain. Furthermore, the treatment ameliorated the VaD-induced damage to the blood-brain barrier (BBB) components, including PECAM-1, PDGFRβ, occludin, claudin-9, and ZO-1. CCR6 activation after VaD, associated with BBB disruption, was diminished by treatment with Lin28a siRNA. The treatment inhibited VaD-induced microglial activity and alleviated BBB damage. Thus, blocking Lin28a may alleviate cognitive impairment caused by VaD.
Intrinsic Shapes of Empathy: Functional Brain Network Topology Encodes Intersubjective Experience and Awareness Traits
Authors: Ebisch Sjoerd J. H. , Scalabrini Andrea , Northoff Georg , Mucci Clara , Sergi Maria Rita , Saggino Aristide , Aquino Antonio , Alparone Francesca R. , Perrucci Mauro Gianni , Gallese Vittorio , Di Plinio Simone
Published: April 2022

Trait empathy is an essential personality feature in the intricacy of typical social inclinations of individuals. Empathy is likely supported by multilevel neuronal network functioning, whereas local topological properties determine network integrity. In the present functional MRI study (N = 116), we aimed to trace empathic traits to the intrinsic brain network architecture. Empathy was conceived as composed of two dimensions within the concept of pre-reflective, intersubjective understanding. Vicarious experience consists of the tendency to resonate with the feelings of other individuals, whereas intuitive understanding refers to a natural awareness of others’ emotional states. Analyses of graph theoretical measures of centrality showed a relationship between the fronto-parietal network and psychometric measures of vicarious experience, whereas intuitive understanding was associated with sensorimotor and subcortical networks. Salience network regions could constitute hubs for information processing underlying both dimensions. The network properties related to empathy dimensions mainly concern inter-network information flow. Moreover, interaction effects implied several sex differences in the relationship between functional network organization and trait empathy. These results reveal that distinct intrinsic topological network features explain individual differences in separate dimensions of intersubjective understanding. The findings could help understand the impact of brain damage or stimulation through alterations of empathy-related network integrity.
Aftereffects to Prism Exposure without Adaptation: A Single Case Study
Authors: Albini Federica , Pisoni Alberto , Salvatore Anna , Calzolari Elena , Casati Carlotta , Marzoli Stefania Bianchi , Falini Andrea , Crespi Sofia Allegra , Godi Claudia , Castellano Antonella , Bolognini Nadia , Vallar Giuseppe
Published: April 2022

Visuo-motor adaptation to optical prisms (Prism Adaptation, PA), displacing the visual scene laterally, is a behavioral method used for the experimental investigation of visuomotor plasticity, and, in clinical settings, for temporarily ameliorating and rehabilitating unilateral spatial neglect. This study investigated the building up of PA, and the presence of the typically occurring subsequent Aftereffects (AEs) in a brain-damaged patient (TMA), suffering from apperceptive agnosia and a right visual half-field defect, with bilateral atrophy of the parieto-occipital cortices, regions involved in PA and AEs. Base-Right prisms and control neutral lenses were used. PA was achieved by repeated pointing movements toward three types of stimuli: visual, auditory, and bimodal audio-visual. The presence and the magnitude of AEs were assessed by proprioceptive, visual, visuo-proprioceptive, and auditory-proprioceptive straight-ahead pointing tasks. The patient’s brain connectivity was investigated by Diffusion Tensor Imaging (DTI). Unlike control participants, TMA did not show any adaptation to prism exposure, but her AEs were largely preserved. These findings indicate that AEs may occur even in the absence of PA, as indexed by the reduction of the pointing error, showing a dissociation between the classical measures of PA and AEs. In the PA process, error reduction, and its feedback, may be less central to the building up of AEs, than the sensorimotor pointing activity per se.
Model to Balance an Acceptable Radon Level Indoors
Authors: Rasmussen Torben Valdbjørn , Cornelius Thomas
Published: April 2022

A theoretical model is presented for balancing an acceptable radon concentration in indoor air. The infiltration of radon from the ground to the indoor air can be controlled by barriers or by lowering the air pressure at the lower zone of the ground slab. Indoor air with a radon concentration higher than that of outdoor air can further be controlled through the effective dilution of indoor air with outdoor air. The theory estimates the allowed radon infiltration from the ground to balance radon at an acceptable level indoors for a given ventilation rate, considering the radon contribution to the indoor air from indoor materials, building materials and the interior. A method using this theory is presented, identifying the necessary airtightness required for a radon barrier to balance the acceptable radon concentration for a building. Barriers include commercially used system solutions, such as bitumen-based radon blockers, wet-room membranes, reinforced fixed mortar pastes, and polyethene membranes. An acceptable indoor radon concentration of between 100 and 300 Bq/m3 in indoor air is used. Barriers are evaluated by their ability to prevent soil gas penetration from the ground in combination with their effect on the building durability, as barriers may create a far more vulnerable building.
Functional Voltage-Gated Sodium Channels Are Present in the Human B Cell Membrane
Authors: Feher Adam , Pócsi Marianna , Papp Ferenc , Szanto Tibor G. , Csoti Agota , Fejes Zsolt , Nagy Béla , Nemes Balázs , Varga Zoltan
Published: April 2022

B cells express various ion channels, but the presence of voltage-gated sodium (NaV) channels has not been confirmed in the plasma membrane yet. In this study, we have identified several NaV channels, which are expressed in the human B cell membrane, by electrophysiological and molecular biology methods. The sensitivity of the detected sodium current to tetrodotoxin was between the values published for TTX-sensitive and TTX-insensitive channels, which suggests the co-existence of multiple NaV1 subtypes in the B cell membrane. This was confirmed by RT-qPCR results, which showed high expression of TTX-sensitive channels along with the lower expression of TTX-insensitive NaV1 channels. The biophysical characteristics of the currents also supported the expression of multiple NaV channels. In addition, we investigated the potential functional role of NaV channels by membrane potential measurements. Removal of Na+ from the extracellular solution caused a reversible hyperpolarization, supporting the role of NaV channels in shaping and maintaining the resting membrane potential. As this study was mainly limited to electrophysiological properties, we cannot exclude the possible non-canonical functions of these channels. This work concludes that the presence of voltage-gated sodium channels in the plasma membrane of human B cells should be recognized and accounted for in the future.
Insulin-Degrading Enzyme, an Under-Estimated Potential Target to Treat Cancer?
Authors: Lesire Laetitia , Leroux Florence , Deprez-Poulain Rebecca , Deprez Benoit
Published: April 2022

Insulin-degrading enzyme (IDE) is a multifunctional protease due to the variety of its substrates, its various cellular locations, its conservation between species and its many non-proteolytic functions. Numerous studies have successfully demonstrated its implication in two main therapeutic areas: metabolic and neuronal diseases. In recent years, several reports have underlined the overexpression of this enzyme in different cancers. Still, the exact role of IDE in the physiopathology of cancer remains to be elucidated. Known as the main enzyme responsible for the degradation of insulin, an essential growth factor for healthy cells and cancer cells, IDE has also been shown to behave like a chaperone and interact with the proteasome. The pharmacological modulation of IDE (siRNA, chemical compounds, etc.) has demonstrated interesting results in cancer models. All these results point towards IDE as a potential target in cancer. In this review, we will discuss evidence of links between IDE and cancer development or resistance, IDE’s functions, catalytic or non-catalytic, in the context of cell proliferation, cancer development and the impact of the pharmacomodulation of IDE via cancer therapeutics.
The Evolution of Complex Muscle Cell In Vitro Models to Study Pathomechanisms and Drug Development of Neuromuscular Disease
Authors: Zschüntzsch Jana , Meyer Stefanie , Shahriyari Mina , Kummer Karsten , Schmidt Matthias , Kummer Susann , Tiburcy Malte
Published: April 2022

Many neuromuscular disease entities possess a significant disease burden and therapeutic options remain limited. Innovative human preclinical models may help to uncover relevant disease mechanisms and enhance the translation of therapeutic findings to strengthen neuromuscular disease precision medicine. By concentrating on idiopathic inflammatory muscle disorders, we summarize the recent evolution of the novel in vitro models to study disease mechanisms and therapeutic strategies. A particular focus is laid on the integration and simulation of multicellular interactions of muscle tissue in disease phenotypes in vitro. Finally, the requirements of a neuromuscular disease drug development workflow are discussed with a particular emphasis on cell sources, co-culture systems (including organoids), functionality, and throughput.
Trophectoderm Transcriptome Analysis in LIN28 Knockdown Ovine Conceptuses Suggests Diverse Roles of the LIN28-let-7 Axis in Placental and Fetal Development
Authors: Ali Asghar , Iqbal Muhammad A. , Abbas Muhammad W. , Bouma Gerrit J. , Anthony Russell V. , Spencer Thomas E. , Winger Quinton A.
Published: April 2022

The proper conceptus elongation in ruminants is critical for the successful placentation and establishment of pregnancy. We have previously shown that the trophectoderm-specific knockdown of LIN28A/B in day 9 ovine blastocysts resulted in increased let-7 miRNAs and reduced conceptus elongation at day 16 of gestation. In this current study, by transcriptome analysis of LIN28A knockdown (AKD) or LIN28B knockdown (BKD) trophectoderm (TE), we explored the downstream target genes of the LIN28-let-7 axis and their roles in the placental and fetal development. We identified 449 differentially expressed genes (DEGs) in AKD TE and 1214 DEGs in BKD TE compared to non-targeting control (NTC). Our analysis further revealed that 210 downregulated genes in AKD TE and 562 downregulated genes in BKD TE were the potential targets of let-7 miRNAs. Moreover, 16 downregulated genes in AKD TE and 57 downregulated and 7 upregulated genes in BKD TE were transcription factors. The DEGs in AKD and BKD TE showed enrichment in the biological processes and pathways critical for placental development and function, and fetal development and growth. The results of this study suggest the potential roles of the LIN28-let-7 axis in placental and fetal development beyond its involvement in trophoblast proliferation and conceptus elongation.
EGFR Targeting TKI-Related Skin Toxicities in a Patient with Darker Skin: A Case Report
Authors: Zereshkian Arman , Thawer Alia , Hwang David M. , Cheng Susanna
Published: April 2022

Epidermal growth factor receptor (EGFR) targeting tyrosine kinase inhibitors (TKIs) can result in significant skin toxicities that may impact patients’ quality of life. While these skin reactions are well documented in patients with lighter skin, there is a paucity of literature and images to guide clinicians in their assessment in patients with darker skin tones. Given that dermatological reactions in patients with darker skin are not well represented, this can result in the undertreatment or mistreatment of these otherwise common toxicities. Herein, we present a case of a female patient with a darker skin tone with metastatic non-small cell lung carcinoma (NSCLC) with EGFR-TKI-related skin toxicity and her clinical course.
Mobile Mammography Services and Underserved Women
Authors: Trivedi Usha , Omofoye Toma S. , Marquez Cindy , Sullivan Callie R. , Benson Diane M. , Whitman Gary J.
Published: April 2022

Breast cancer, the second most common cause of cancer in women, affects people across different ages, ethnicities, and incomes. However, while all women have some risk of breast cancer, studies have found that some populations are more vulnerable to poor breast cancer outcomes. Specifically, women with lower socioeconomic status and of Black and Hispanic ethnicity have been found to have more advanced stages of cancer upon diagnosis. These findings correlate with studies that have found decreased use of screening mammography services in these underserved populations. To alleviate these healthcare disparities, mobile mammography units are well positioned to provide convenient screening services to enable earlier detection of breast cancer. Mobile mammography services have been operating since the 1970s, and, in the current pandemic, they may be extremely helpful. The COVID-19 pandemic has significantly disrupted necessary screening services, and reinstatement and implementation of accessible mobile screenings may help to alleviate the impact of missed screenings. This review discusses the history and benefits of mobile mammography, especially for underserved women.
Retained Placenta Percreta with Acquired Uterine Arteriovenous Malformation—Case Report and Short Review of the Literature
Authors: Butureanu Tudor , Balan Raluca Anca , Socolov Razvan , Ioanid Nicolae , Socolov Demetra , Gafitanu Dumitru
Published: April 2022

Placenta accreta spectrum disorder (PAS) has an increased frequency due to the high number of cesarean sections. The abnormal placentation associated with a retained placenta can cause persistent uterine bleeding, with ultrasound Doppler examination being the main choice to assess the uterine hemorrhage. An acquired uterine arteriovenous malformation (AVM) may occur because of uterine trauma, spontaneous abortion, dilation and curettage, endometrial carcinoma or gestational trophoblastic disease. The treatment for abnormal placentation associated with AVM can be conservative, represented by methotrexate therapy, arterial embolization, uterine curettage, hysteroscopic loop resection or radical, which takes into consideration total hysterectomy. Therapeutic management always considers the degree of placental invasion, the patient hemodynamic state and fertility preservation. Considering the aspects described, we present a case of retained placenta percreta associated with acquired uterine AVM, with imagistic and clinical features suggestive of a gestational trophoblastic disease, successfully treated by hysterectomy, along with a small review of the literature, as only a few publications have reported a similar association of diagnostics and therapy.
How Potential Evapotranspiration Regulates the Response of Canopy Transpiration to Soil Moisture and Leaf Area Index of the Boreal Larch Forest in China
Authors: Xu Zhipeng , Man Xiuling , Cai Tijiu , Shang Youxian
Published: April 2022

Transpiration is a critical component of the hydrological cycle in the terrestrial forest ecosystem. However, how potential evapotranspiration regulates the response of canopy transpiration to soil moisture and leaf area index of the boreal larch forest in China has rarely been evaluated. The present study was conducted in the larch (Larix gmelinii (Rupr.) Rupr.) forest, which is a typical boreal forest in China. The canopy transpiration was measured using sap flow techniques from May to September in 2021 and simultaneously observing the meteorological variables, leaf area index (LAI) and soil moisture (SWC). The results showed that there were significant differences in canopy transpiration of Larix gmelinii among the months. The correlation and regression analysis indicated that canopy transpiration was mainly influenced by potential evapotranspiration (PET), while the effect of soil moisture on canopy transpiration was lowest compared with other environmental factors. Furthermore, our results revealed that the effect of PET on canopy transpiration was not regulated by soil moisture when soil moisture exceeded 0.2 cm3 cm−3. More importantly, under the condition of sufficient soil moisture, it was demonstrated that the response of canopy transpiration to leaf area index was limited when PET exceeded 9 mm/day. These results provide valuable implications for supporting forest management and water resource utilization in the boreal forest ecosystem under the context of global warming.
Does Classification-Based Forest Management Promote Forest Restoration? Evidence from China’s Ecological Welfare Forestland Certification Program
Authors: Xu Chang , Lin Fanli , Zhu Chenghao , Li Chaozhu , Cheng Baodong
Published: April 2022

Classification-based forest management (CFM) is generally regarded as an important political means of achieving sustainable forest development. However, in the upsurge of publicly managed forest devolution, the impact of CFM policies on forestland restoration remains uncertain and needs to be explored. This study contributes to the scant literature on this topic in China, where CFM has long been implemented based on the ecological welfare forestland (EWF) certification program. We use provincial data from China to examine the relationship between EWF-certified areas and forest restoration. Based on inter-provincial panel data from the third to the ninth consecutive forest resource inventories in China (1984–2018), we use a dynamic spatial autoregressive model to analyze the impact of forest classification management on forest restoration. The results show that, contrary to appearances, increasing EWF-certified areas promotes forest restoration. However, after controlling for other possible influencing factors, increasing EWF-certified areas plays a minimal role in promoting forest restoration and regrowth by inhibiting investment in forest management and even has a negative impact on forest restoration in the southern collective forest area.
NTH2 1271_1272delTA Gene Disruption Results in Salt Tolerance in Saccharomyces cerevisiae
Authors: Hernández-Soto Alejandro , Delgado-Navarro José Pablo , Benavides-Acevedo Miguel , Paniagua Sergio A. , Gatica-Arias Andres
Published: April 2022

Trehalose is a common energy reservoir, and its accumulation results in osmotic protection. This sugar can accumulate through its synthesis or slow degradation of the reservoir by trehalase enzymes. Saccharomyces cerevisiae contains two neutral trehalases, NTH1 and NTH2, responsible for 75% and 25% of the enzymatic metabolism. We were interested in the loss-of-function of both enzymes with CRISPR/Cas9. The later NTH2 was of great importance since it is responsible for minor metabolic degradation of this sugar. It was believed that losing its functionality results in limited osmotic protection. We constructed an osmotolerant superior yeast capable of growing in 0.85 M NaCl after independent nth21271_1272delTA mutation by CRISPR/Cas9 technology, compared with nth1 893_894insT and wild type. We suggest that this yeast model could give clues to breeding commercial yeast resulting in non-GMO salinity-tolerant strains.
New Solutions of Nonlinear Dispersive Equation in Higher-Dimensional Space with Three Types of Local Derivatives
Authors: Akgül Ali , Hashemi Mir Sajjad , Jarad Fahd
Published: April 2022

The aim of this paper is to use the Nucci’s reduction method to obtain some novel exact solutions to the s-dimensional generalized nonlinear dispersive mK(m,n) equation. To the best of the authors’ knowledge, this paper is the first work on the study of differential equations with local derivatives using the reduction technique. This higher-dimensional equation is considered with three types of local derivatives in the temporal sense. Different types of exact solutions in five cases are reported. Furthermore, with the help of the Maple package, the solutions found in this study are verified. Finally, several interesting 3D, 2D and density plots are demonstrated to visualize the nonlinear wave structures more efficiently.
Modelling Quintessential Inflation in Palatini-Modified Gravity
Authors: Dimopoulos Konstantinos , Karam Alexandros , López Samuel , Tomberg Eemeli
Published: April 2022

We study a model of quintessential inflation constructed in R2-modified gravity with a non-minimally coupled scalar field, in the Palatini formalism. Our non-minimal inflaton field is characterised by a simple exponential potential. We find that successful quintessential inflation can be achieved with no fine-tuning of the model parameters. Predictions of the characteristics of dark energy will be tested by observations in the near future, while contrasting with existing observations provides insights on the modified gravity background, such as the value of the non-minimal coupling and its running.
Study of Ag Nanoparticles in a Polyacrylamide Hydrogel Dosimeters by Optical Technique
Authors: Soliman Yasser S. , Tadros Soad M. , Beshir Wafaa B. , Saad Gamal R. , Gallo Salvatore , Ali Laila I. , Naoum Magdi M.
Published: April 2022

The dosimetric characteristics of hydrogel dosimeters based on polyacrylamide (PAC) as a capping agent incorporating silver nitrate as a radiation-sensitive material are investigated using UV-Vis spectrophotometry within the dose range 0–100 Gy. Glycerol was used in the hydrogel matrix to promote the dosimetric response and increase the radiation sensitivity. Upon exposing the PAC hydrogel to γ-ray, it exhibits a Surface Plasmon Resonance (SPR) band at 453 nm, and its intensity increases linearly with absorbed doses up to 100 Gy. The results are compared with the silver nitrate gel dosimeter. Glycerol of 15% in the hydrogel matrix enhances the radiation sensitivity by about 30%. PAC hydrogel dosimeter can be considered a near water equivalent material in the 400 keV–20 MeV photon energy range. At doses less than 15 Gy, the PAC hydrogel dosimeter retains higher radiation sensitivity than the gel dosimeter. The total uncertainty (2σ) of the dose estimated using this hydrogel is about 4%. These results may support the validity of using this hydrogel as a dosimeter to verify radiotherapy techniques and dose monitoring during blood irradiation.
Identification and Somatic Characterization of the Germline PTEN Promoter Variant rs34149102 in a Family with Gastrointestinal and Breast Tumors
Authors: Disciglio Vittoria , Sanese Paola , Fasano Candida , Lotesoriere Claudio , Valentini Anna , Forte Giovanna , Lepore Signorile Martina , De Marco Katia , Grossi Valentina , Lolli Ivan , Cariola Filomena , Simone Cristiano
Published: April 2022

Genetic variants located in non-coding regions can affect processes that regulate protein expression, functionally contributing to human disease. Germline heterozygous mutations in the non-coding region of the PTEN gene have been previously identified in patients with PTEN hamartoma tumor syndrome (PHTS) diagnosed with breast, thyroid, and/or endometrial cancer. In this study, we report a PTEN promoter variant (rs34149102 A allele) that was identified by direct sequencing in an Italian family with a history of gastroesophageal junction (GEJ) adenocarcinoma and breast cancer. In order to investigate the putative functional role of the rs34149102 A allele variant, we evaluated the status of PTEN alterations at the somatic level. We found that PTEN protein expression was absent in the GEJ adenocarcinoma tissue of the index case. Moreover, we detected the occurrence of copy number loss involving the PTEN rs34149102 major C allele in tumor tissue, revealing that the second allele was somatically inactivated. This variant is located within an active regulatory region of the PTEN core promoter, and in silico analysis suggests that it may affect the binding of the nuclear transcription factor MAZ and hence PTEN expression. Overall, these results reveal the functional role of the PTEN promoter rs34149102 A allele variant in the modulation of PTEN protein expression and highlight its contribution to hereditary cancer risk.
Trends in Homicide Hospitalization and Mortality in Taiwan, 1998–2015
Authors: Hsing Shih-Chun , Chen Chu-Chieh , Huang Shi-Hao , Huang Yao-Ching , Wang Bing-Long , Chung Chi-Hsiang , Sun Chien-An , Chien Wu-Chien , Wu Gwo-Jang
Published: April 2022

In Taiwan, the national research on homicide is rare, mostly discussing the issue of child abuse. We sought to better understand the characteristics and risk factors of homicide through a retrospective cohort study from 1998 to 2015. “Child battering and other maltreatment” ranked first for the 0–4 age group and second for the 5–14 age group. The hospital mortality was 511 deaths. We found that the 25–44 age group had the highest risk and accounted for 44.76% of hospitalization. The most common causes were “fight, brawl, and rape” (49.12%), “cutting and piercing instruments,” (13.16%) and “child battering and other forms of maltreatment” (4.72%). Additionally, the percentages of “fight, brawl, and rape,” “firearms and explosives,” and “cutting and piercing instruments” were significantly higher among males than among females. The percentages of “hanging and strangulation,” “corrosive or caustic substance,” “child battering and other maltreatment,” “submersion,” and “poisoning” were significantly higher among females than males. Factors associated with homicide in-hospital mortality included gender, age, low income, catastrophic disease, Charlson comorbidity index score, urbanization level, hospital level, classification of hospitalization, and surgery. Overall, the trend of hospitalization rates due to homicide decreased both by gender and age group, except for the 0–4 age group: cause of homicide first, hanging and strangulation second, firearms and explosives third; type of injury, hospitalized patients with “vascular injuries” first, joint and muscle sprain, and intracranial, chest, and abdominal pelvic injuries second, and “burns” third with a higher risk of death. Homicide reduction requires a comprehensive strategy beyond specific victim groups. Interagency collaboration should be strengthened, especially between law enforcement/criminal justice and public health.
Association between Social Isolation and Total Mortality after the Great East Japan Earthquake in Iwate Prefecture: Findings from the TMM CommCohort Study
Authors: Kotozaki Yuka , Tanno Kozo , Sakata Kiyomi , Otsuka Kotaro , Sasaki Ryohei , Takanashi Nobuyuki , Satoh Mamoru , Shimizu Atsushi , Sasaki Makoto
Published: April 2022

This study aimed to investigate whether social isolation is associated with mortality, together with the effect of the Great East Japan Earthquake on mortality, due to the social isolation of community residents living in the affected areas, using data from the Tohoku Medical Megabank Project Community-Based Cohort Study. A total of 22,933 participants (8059 men and 14,874 women), who were free from cancer and cardiovascular disease, were followed up with death as an endpoint for five years. Social isolation was assessed using the Lubben Social Network Scale (cut-off, 11/12). Using Cox proportional hazards models, hazard ratios (HRs) of total mortality and 95% confidence intervals (CIs) associated with social isolation (no isolation as the reference) were estimated. The latter was significantly associated with an increased risk of total mortality (1.38 (1.04–1.83) in men and 1.49 (1.02–2.19) in women). Moreover, among those with social isolation, the risk of mortality was significantly higher, especially for women with house damage and men who had experienced a death in the family. The disaster may have raised the risk of mortality due to social isolation.
The Portrait of Cyberchondria—A Cross-Sectional Online Study on Factors Related to Health Anxiety and Cyberchondria in Polish Population during SARS-CoV-2 Pandemic
Authors: Ciułkowicz Marta , Misiak Błażej , Szcześniak Dorota , Grzebieluch Jolanta , Maciaszek Julian , Rymaszewska Joanna
Published: April 2022

The SARS-CoV-2 pandemic has served as a magnifying glass for cyberchondria, while the internet emerged as one of the main sources of medical information and support. The core ambition of this study was to estimate the level of cyberchondria and describe the socio-demographic, clinical and pandemic-related factors affecting its severity amid the SARS-CoV-2 pandemic. A cross-sectional study was performed between 16 May 2020 and 29 December 2020 in Poland within a sample of 538 adult internet users. The online survey tool included a Polish adaptation of the Cyberchondria Severity Scale (CSS-PL) and the Short Health Anxiety Inventory (SHAI), complemented with a set of questions covering sociodemographic, clinical and pandemic-related factors. Participants were clustered according to severity of health anxiety and cyberchondria symptoms. The performed binary logistic regression indicated professional inactivity, having a chronic mental disorder and subjectively limited access to healthcare due to COVID-19 to be key determinants of severe health anxiety and cyberchondria. Cyberchondria might be a remarkable public health issue as large proportion of respondents from the analyzed sample population of internet users met the criteria for severe symptoms. Key determinants of intense cyberchondria corresponded with employment stability, mental resilience and accessibility of healthcare services, which could be greatly challenged amid the pandemic.
Dentists’ Competence and Knowledge on Domestic Violence and How to Improve It—A Review
Authors: Bregulla Jana Lauren , Hanisch Marcel , Pfleiderer Bettina
Published: April 2022

Domestic violence (DV) is an important public health topic with a high prevalence in society. Dentists are also frontline responders to DV, as they not only treat victims of DV with dental injuries, but they can also screen for the presence of DV because they see patients for regular check-ups. Using the WHO definition, which describes domestic violence as intimate partner violence, 17 papers could be included in our analyses. The results of this review clearly indicated that although dentists, as members of the health care sector, are important frontline responders to DV, they are neither trained adequately at medical school nor do most feel competent enough to ask victims about DV or support them as needed. DV is often not taught at dentistry schools at all. The aims of this review were to provide an overview of existing literature on dentists’ knowledge and beliefs regarding DV, whether and how DV is taught in medical education and to give recommendations on how to improve the education of dentists on this topic. Based on our findings, we recommend that DV education should be mandatory at dentistry schools and in further training for dentists with a focus on communication with victims, how DV can be identified and how to support victims well.
Life Conditions as Mediators of Welfare State Effect on Mental Wellbeing among Oldest Old in Europe
Authors: Cresswell-Smith Johanna , Wahlbeck Kristian , Kalseth Jorid
Published: April 2022

Background: Mental wellbeing is formed by our daily environments, which are, in turn, influenced by public policies, such as the welfare state. This paper looks at how different aspects of life conditions may mediate the welfare state effect on mental wellbeing in oldest old age. Methods: Data were extracted from Round 6 of the European Social Survey (2012). The dataset comprised of 2058 people aged 80 years and older from 24 countries. Mediation analyses determined possible links between the welfare state, including eleven intervening variables representing life conditions and five mental wellbeing dimensions. Results: Our study confirms that the higher the level of welfare state, the better mental wellbeing, irrespective of dimension. Although several life conditions were found to mediate the welfare state effect on mental wellbeing, subjective general health, coping with income and place in society were the most important intervening variables. Conclusions: All three variables centre around supporting autonomy in the oldest old age. By teasing out how the welfare state influences mental wellbeing in the oldest old, we can better understand the many drivers of wellbeing and enable evidence informed age-friendly policy making.
Episodic Memory Performance Modifies the Strength of the Age–Brain Structure Relationship
Authors: Richmond Lauren L. , Brackins Timothy , Rajaram Suparna
Published: April 2022

The bivariate relationships between brain structure, age, and episodic memory performance are well understood. Advancing age and poorer episodic memory performance are each associated with smaller brain volumes and lower cortical thickness measures, respectively. Advancing age is also known to be associated with poorer episodic memory task scores on average. However, the simultaneous interrelationship between all three factors—brain structure, age, and episodic memory—is not as well understood. We tested the hypothesis that the preservation of episodic memory function would modify the typical trajectory of age-related brain volume loss in regions known to support episodic memory function using linear mixed models in a large adult lifespan sample. We found that the model allowing for age and episodic memory scores to interact predicted the hippocampal volume better than simpler models. Furthermore, we found that a model including a fixed effect for age and episodic memory scores (but without the inclusion of the interaction term) predicted the cortical volumes marginally better than a simpler model in the prefrontal regions and significantly better in the posterior parietal regions. Finally, we observed that a model containing only a fixed effect for age (e.g., without the inclusion of memory scores) predicted the cortical thickness estimates and regional volume in a non-memory control region. Together, our findings provide support for the idea that the preservation of memory function in late life can buffer against typical patterns of age-related brain volume loss in regions known to support episodic memory.
Tuberculosis Co-Infection Is Common in Patients Requiring Hospitalization for COVID-19 in Belarus: Mixed-Methods Study
Authors: Sereda Yuliia , Korotych Oleksandr , Klimuk Dzmitry , Zhurkin Dzmitry , Solodovnikova Varvara , Grzemska Malgorzata , Grankov Viatcheslav , Hurevich Hennadz , Yedilbayev Askar , Skrahina Alena
Published: April 2022

A significant drop in tuberculosis (TB) case-finding has been widely reported during the period of the COVID-19 pandemic. To address a decrease in TB notification, Belarus introduced laboratory TB testing in patients with the laboratory-confirmed coronavirus disease 2019 (COVID-19). We conducted a secondary analysis of health records among 844 patients with laboratory-confirmed COVID-19 diagnosis who were admitted to repurposed departments at TB hospitals and who were tested by Xpert MTB/RIF (Cepheid Inc., Sunnyvale, CA, USA) in five Belarus regions between April and October 2021. Quantitative analysis followed by 13 individual interviews with health managers, physicians, and nurses participating in the intervention. Most patients were male (64%) and mean age was 43.5 ± 16 years. One in twenty (n = 47, 5.6%) patients were co-infected with active pulmonary TB, and over one-third of them (n = 18) had rifampicin resistance. In-hospital mortality was comparable in patients with and without TB co-infection (2.1% and 2.3% respectively, p > 0.99). Laboratory TB testing among patients with COVID-19 at repurposed departments of TB hospitals is feasible in Belarus and may improve TB case-finding.