bluuebunny/crossref_metadata_2025 huggingface dataset:
Electric Vehicles Charging Management System for Optimal Exploitation of Photovoltaic Energy Sources Considering Vehicle-to-Vehicle Mode
Authors: Lo Franco Francesco , Mandrioli Riccardo , Ricco Mattia , Monteiro Vítor , Monteiro Luís F. C. , Afonso João L. , Grandi Gabriele
Published: November 2021

The growing penetration of distributed renewable energy sources (RES) together with the increasing number of new electric vehicle (EV) model registrations is playing a significant role in zero-carbon energy communities’ development. However, the ever-larger share of intermittent renewable power plants, combined with the high and uncontrolled aggregate EV charging demand, requires an evolution toward new planning and management paradigms of energy districts. Thus, in this context, this paper proposes novel smart charging (SC) techniques that aim to integrate as much as possible RES generation and EV charging demand at the local level, synergically acting on power flows and avoiding detrimental effects on the electrical power system. To make this possible, a centralized charging management system (CMS) capable of individually modulating each charging power of plugged EVs is presented in this paper. The CMS aims to maximize the charging self-consumption from local RES, flattening the peak power required to the external grid. Moreover, the CMS guarantees an overall good state of charge (SOC) at departure time for all the vehicles without requiring additional energy from the grid even under low RES power availability conditions. Two methods that differ as a function of the EV power flow direction are proposed. The first SC only involves unidirectional power flow, while the second one also considers bidirectional power flow among vehicles, operating in vehicle-to-vehicle (V2V) mode. Finally, simulations, which are presented considering an actual case study, validate the SC effects on a reference scenario consisting of an industrial area having a photovoltaic (PV) plant, non-modulable electrical loads, and EV charging stations (CS). Results are collected and performance improvements by operating the different SC methods are compared and described in detail in this paper.
LPS Stimulation Induces Small Heterodimer Partner Expression Through the AMPK-NRF2 Pathway in Large Yellow Croaker (Larimichthys crocea)
Authors: Du Jianlong , Xiang Xiaojun , Xu Dan , Cui Kun , Pang Yuning , Xu Wei , Mai Kangsen , Ai Qinghui
Published: November 2021

The mall heterodimer partner (SHP) plays an important regulatory role in mammal inflammation. The main objective of this study was to investigate the response of SHP to inflammatory stimulation and its underlying mechanism. The shp gene from large yellow croakers, was cloned, and this gene is mainly expressed in the liver and intestine. Lipopolysaccharide (LPS) stimulation induced the mRNA expression and protein level of SHP in macrophages of large yellow croakers. Overexpression of SHP significantly decreased mRNA expression of tnfα, il-1β, il-6 and cox2 induced by LPS treatment in macrophages. LPS stimulation increased the phosphorylation level of Adenosine 5’-monophosphate (AMP)-activated protein kinase (AMPK) in macrophages. AMPK inhibitor treatment significantly decreased the expression of SHP induced by LPS while AMPK activator significantly increased the expression of SHP. The nuclear factor-erythroid 2-related factor 2 (NRF2) increased the promoter activity of SHP in large yellow croakers and the level of nuclear NRF2 was increased by LPS stimulation and AMPK activation. NRF2 inhibitor treatment significantly decreased mRNA expression of shp induced by LPS and AMPK activator. In conclusion, LPS can induce SHP expression by activating the AMPK-NRF2 pathway while SHP could negatively regulate LPS-induced inflammation in large yellow croakers. This study may be benefit to the development of immunology of marine fish and provide new ideas for inflammation-related diseases.
Soot Volume Fraction Measurements by Auto-Compensating Laser-Induced Incandescence in Diffusion Flames Generated by Ethylene Pool Fire
Authors: Cruz Juan J. , Verdugo Ignacio , Gutiérrez-Cáceres Nicolás , Escudero Felipe , Demarco Rodrigo , Liu Fengshan , Yon Jérôme , Chen Dongping , Fuentes Andrés
Published: November 2021

The main characteristics of pool fire flames are flame height, air entrainment, pulsation of the flame, formation and properties of soot particles, mass burning rate, radiation feedback to the pool surface, and the amount of pollutants including soot released to the environment. In this type of buoyancy controlled flames, the soot content produced and their subsequent thermal radiation feedback to the pool surface are key to determine the self-sustainability of the flame, their mass burning rate and the heat release rate. The accurate characterization of these flames is an involved task, specially for modelers due to the difficulty of imposing adequate boundary conditions. For this reason, efforts are being made to design experimental campaigns with well-controlled conditions for their reliable repeatability, reproducibility and replicability. In this work, we characterized the production of soot in a surrogate pool fire. This is emulated by a bench-scale porous burner fueled with pure ethylene burning in still air. The flame stability was characterized with high temporal and spatial resolution by using a CMOS camera and a fast photodiode. The results show that the flame exhibit a time-varying propagation behavior with a periodic separation of the reactive zone. Soot volume fraction distributions were measured at nine locations along the flame centerline from 20 to 100 mm above the burner exit using the auto-compensating laser-induced incandescence (AC-LII) technique. The mean, standard deviation and probability density function of soot volume fraction were determined. Soot volume fraction presents an increasing tendency with the height above the burner, in spite of a local decrease at 90 mm which is approximately the position separating the lower and attached portion of the flame from the higher more intermittent one. The results of this work provide a valuable data set for validating soot production models in pool fire configurations.
Acute-on-Chronic Liver Failure: Pathophysiological Mechanisms and Management
Authors: Khanam Arshi , Kottilil Shyam
Published: November 2021

Acute-on-chronic liver failure (ACLF) is a multifaceted condition with poor treatment options and high short-term mortality. ACLF can develop in patients with or without liver cirrhosis, where patients with decompensated cirrhosis display a higher risk of short-term mortality. Pathophysiological mechanisms include systemic inflammation due to bacterial and fungal infections and acute hepatic insult with drug, alcohol, and viral hepatitis. Cryptogenic factors also contribute to the development of ACLF. The clinical outcome of patients with ACLF gets further complicated by the occurrence of variceal hemorrhage, hepatorenal syndrome, hepatic encephalopathy, and systemic immune dysfunction. Regardless of the better understanding of pathophysiological mechanisms, no specific and definitive treatment is available except for liver transplantation. The recent approach of regenerative medicine using mesenchymal stem cells (MSCs) could be advantageous for the treatment of ACLF as these cells can downregulate inflammatory response by inducing antiinflammatory events and prevent hepatic damage and fibrosis by inhibiting hepatic stellate cell activation and collagen synthesis. Moreover, MSCs are involved in tissue repair by the process of liver regeneration. Considering the broad therapeutic potential of MSCs, it can serve as an alternative treatment to liver transplant in the near future, if promising results are achieved.
Mechanisms and Efficacy of Intravenous Lipid Emulsion Treatment for Systemic Toxicity From Local Anesthetics
Authors: Liu Yang , Zhang Jing , Yu Peng , Niu Jiangfeng , Yu Shuchun
Published: November 2021

Local anesthetics are widely used clinically for perioperative analgesia to achieve comfort in medical treatment. However, when the concentration of local anesthetics in the blood exceeds the tolerance of the body, local anesthetic systemic toxicity (LAST) will occur. With the development and popularization of positioning technology under direct ultrasound, the risks and cases of LAST associated with direct entry of the anesthetic into the blood vessel have been reduced. Clinical occurrence of LAST usually presents as a series of severe toxic reactions such as myocardial depression, which is life-threatening. In addition to basic life support (airway management, advanced cardiac life support, etc.), intravenous lipid emulsion (ILE) has been introduced as a treatment option in recent years and has gradually become the first-line treatment for LAST. This review introduces the mechanisms of LAST and identifies the clinical symptoms displayed by the central nervous system and cardiovascular system. The paper features the multimodal mechanism of LAST reversal by ILE, describes research progress in the field, and identifies other anesthetics involved in the resuscitation process of LAST. Finally, the review presents key issues in lipid therapy. Although ILE has achieved notable success in the treatment of LAST, adverse reactions and contraindications also exist; therefore, ILE requires a high degree of attention during use. More in-depth research on the treatment mechanism of ILE, the resuscitation dosage and method of ILE, and the combined use with other resuscitation measures is needed to improve the efficacy and safety of clinical resuscitation after LAST in the future.
Increased Levels of sCD30 Have No Impact on the Incidence of Early ABMR and Long-Term Outcome in Intermediate-Risk Renal Transplant Patients With Preformed DSA
Authors: Drasch Thomas , Bach Christian , Luber Markus , Spriewald Bernd , Utpatel Kirsten , Büttner-Herold Maike , Banas Bernhard , Zecher Daniel
Published: November 2021

Background: It is still incompletely understood why some patients with preformed donor-specific anti-HLA antibodies (DSA) have reduced kidney allograft survival secondary to antibody-mediated rejection (ABMR), whereas many DSA-positive patients have favorable long-term outcomes. Elevated levels of soluble CD30 (sCD30) have emerged as a promising biomarker indicating deleterious T-cell help in conjunction with DSA in immunologically high-risk patients. We hypothesized that this would also be true in intermediate-risk patients.

Methods: We retrospectively analyzed pre-transplant sera from 287 CDC-crossmatch negative patients treated with basiliximab induction and tacrolimus-based maintenance therapy for the presence of DSA and sCD30. The incidence of ABMR according to the Banff 2019 classification and death-censored allograft survival were determined.

Results: During a median follow-up of 7.4 years, allograft survival was significantly lower in DSA-positive as compared to DSA-negative patients (p < 0.001). In DSA-positive patients, most pronounced in those with strong DSA (MFI > 5,000), increased levels of sCD30 were associated with accelerated graft loss compared to patients with low sCD30 (3-year allograft survival 75 vs. 95%). Long-term survival, however, was comparable in DSA-positive patients irrespective of sCD30 status. Likewise, the incidence of early ABMR and lesion score characteristics were comparable between sCD30-positive and sCD30-negative patients with DSA. Finally, increased sCD30 levels were not predictive for early persistence of DSA.

Conclusion: Preformed DSA are associated with an increased risk for ABMR and long-term graft loss independent of sCD30 levels in intermediate-risk kidney transplant patients.
Nucleic Acid-Based Treatments Against COVID-19: Potential Efficacy of Aptamers and siRNAs
Authors: Khanali Javad , Azangou-Khyavy Mohammadreza , Asaadi Yasaman , Jamalkhah Monire , Kiani Jafar
Published: November 2021

Despite significant efforts, there are currently no approved treatments for COVID-19. However, biotechnological approaches appear to be promising in the treatment of the disease. Accordingly, nucleic acid-based treatments including aptamers and siRNAs are candidates that might be effective in COVID-19 treatment. Aptamers can hamper entry and replication stages of the SARS-CoV-2 infection, while siRNAs can cleave the viral genomic and subgenomic RNAs to inhibit the viral life cycle and reduce viral loads. As a conjugated molecule, aptamer–siRNA chimeras have proven to be dual-functioning antiviral therapy, acting both as virus-neutralizing and replication-interfering agents as well as being a siRNA targeted delivery approach. Previous successful applications of these compounds against various stages of the pathogenesis of diseases and viral infections, besides their advantages over other alternatives, might provide sufficient rationale for the application of these nucleic acid-based drugs against the SARS-CoV-2. However, none of them are devoid of limitations. Here, the literature was reviewed to assess the plausibility of using aptamers, siRNAs, and aptamer–siRNA chimeras against the SARS-CoV-2 based on their previously established effectiveness, and discussing challenges lie in applying these molecules.
Early Detection and Investigation of Extracellular Vesicles Biomarkers in Breast Cancer
Authors: Bandini Erika , Rossi Tania , Scarpi Emanuela , Gallerani Giulia , Vannini Ivan , Salvi Samanta , Azzali Irene , Melloni Mattia , Salucci Sara , Battistelli Michela , Serra Patrizia , Maltoni Roberta , Cho William C. , Fabbri Francesco
Published: November 2021

Breast cancer (BC) is the most commonly diagnosed malignant tumor in women worldwide, and the leading cause of cancer death in the female population. The percentage of patients experiencing poor prognosis along with the risk of developing metastasis remains high, also affecting the resistance to current main therapies. Cancer progression and metastatic development are no longer due entirely to their intrinsic characteristics, but also regulated by signals derived from cells of the tumor microenvironment. Extracellular vesicles (EVs) packed with DNA, RNA, and proteins, are the most attractive targets for both diagnostic and therapeutic applications, and represent a decisive challenge as liquid biopsy-based markers. Here we performed a study based on a multiplexed phenotyping flow cytometric approach to characterize BC-derived EVs from BC patients and cell lines, through the detection of multiple antigens. Our data reveal the expression of EVs-related biomarkers derived from BC patient plasma and cell line supernatants, suggesting that EVs could be exploited for characterizing and monitoring disease progression.
Prefrontal-Striatal Mechanisms of Behavioral Impulsivity During Consumption of Delayed Real Liquid Rewards
Authors: Misonou Ayaka , Jimura Koji
Published: November 2021

Intertemporal choice involves the evaluation of future rewards and reflects behavioral impulsivity. After choosing a delayed reward in an intertemporal choice, a behavioral agent waits for, receives, and then consumes the reward. The current study focused on the consumption of the delayed reward and examined the neural mechanisms of behavioral impulsivity. In humans consuming delayed real liquid rewards in an intertemporal choice, the ventral striatum (VS) showed differential activity between anterior (aVS) and posterior (pVS) regions depending on the degree of behavioral impulsivity. Additionally, impulsive individuals showed activity in the anterior prefrontal cortex (aPFC). An analysis of task-related effective connectivity based on psychophysiological interaction (PPI) revealed that PPI was robust from the aPFC to pVS, but not in the opposite direction. On the other hand, strong bidirectional PPIs were observed between the aVS and pVS, but PPIs from the pVS to aVS were enhanced in impulsive individuals. These results suggest that behavioral impulsivity is reflected in aPFC-VS mechanisms during the consumption of delayed real liquid rewards.
Transcranial Magnetic Stimulation Alleviates Levodopa-Induced Dyskinesia in Parkinson's Disease and the Related Mechanisms: A Mini-Review
Authors: Wu Yi , Cao Xue-bing , Zeng Wei-qi , Zhai Heng , Zhang Xiao-qian , Yang Xiao-man , Cheng Chi , Wang Jia-ling , Yang Xiao-mei , Xu Yan
Published: November 2021

After long-term use of levodopa, Parkinson's patients almost inevitably develop dyskinesia, a kind of drug side effect manifesting as uncontrollable choreic movements and dystonia, which could be crippling yet have limited therapeutic options. Transcranial magnetic stimulation is the most widely studied non-invasive neuromodulation technology to treat levodopa-induced dyskinesia. Many studies have shown that transcranial magnetic stimulation has beneficial effects on levodopa-induced dyskinesia and is patient-tolerable, barely with reported adverse effects. Changes in brain connectivity, neuroplasticity, neurotransmitter, neurorestoration, and blood flow modulation could play crucial roles in the efficacy of transcranial magnetic stimulation for levodopa-induced dyskinesia. The appearance of new modes and application for emerging targets are possible solutions for transcranial magnetic stimulation to achieve sustained efficacy. Since the sample size in all available studies is small, more randomized double-blind controlled studies are needed to elucidate the specific treatment mechanisms and optimize treatment parameters.
A Pediatric Sepsis Protocol Reduced Mortality and Dysfunctions in a Brazilian Public Hospital
Authors: Medeiros Daniela Nasu Monteiro , Mafra Ana Carolina Cintra Nunes , Carcillo Joseph Anthony , Troster Eduardo Juan
Published: November 2021

Introduction:Few studies in the literature discuss the benefits of compliance with sepsis bundles in hospitals in low- and middle-income countries, where resources are limited and mortality is high.

Methods:This is a retrospective cohort study conducted at a public hospital in a low-income region in Brazil. We evaluated whether completion of a sepsis bundle is associated with reduced in-hospital mortality for sepsis, severe sepsis, and septic shock, as well as prevention of septic shock and organ dysfunction. Bundle compliance required the completion of three items: (1) obtaining blood count and culture, arterial or venous blood gases, and arterial or venous lactate levels; (2) antibiotic infusion within the first hour of diagnosis; and (3) infusion of 10–20 ml/kg saline solution within the first hour of diagnosis.

Results:A total of 548 children with sepsis, severe sepsis, or septic shock who were treated at the emergency room from February 2008 to August of 2016 were included in the study. Of those, 371 patients were included in the protocol group and had a lower median length of stay (3 days vs. 11 days;p< 0.001), fewer organ dysfunctions during hospitalization (0 vs. 2,p< 0.001), and a lower probability of developing septic shock. According to a propensity score analysis, mortality was lower during the post-implementation period [2.75 vs. 15.4% (RR 95%IC 0.13 (0.06, 0.27);p< 0.001)].

Conclusions:A simple and low-cost protocol was feasible and yielded good results at a general hospital in a low-income region in Brazil. Protocol use resulted in decreased mortality and progression of dysfunctions and was associated with a reduced probability of developing septic shock.
Intermittent Bi-Daily Sub-cutaneous Teriparatide Administration in Children With Hypoparathyroidism: A Single-Center Experience
Authors: Bernardor Julie , Flammier Sacha , Cabet Sara , Lemoine Sandrine , Chapurlat Roland , Molin Arnaud , Bertholet-Thomas Aurélia , Bacchetta Justine
Published: November 2021

Introduction: The use of teriparatide has been reported in children with hypoparathyroidism as an investigational physiologic replacement therapy.

Methods: We aimed to retrospectively report our pediatric experience of bi-daily sub-cutaneous teriparatide. Results are presented as median (25th−75th quartile). As part of the routine follow-up of these patients with hypoparathyroidism, total calcium at H0 (i.e., just before injection) and H4 (i.e., 4 h after teriparatide injection) and other biomarker parameters were regularly assessed.

Results: At a median age of 10.7 (8.1–12.6) years, an estimated glomerular filtration rate (eGFR) of 110 (95–118) mL/min/1.73 m2, calcium levels of 1.87 (1.81–1.96) mmol/L and an age-standardized phosphate of 3.8 (2.5–4.9) SDS, teriparatide therapy was introduced in 10 patients at the dose of 1.1 (0.7–1.5) μg/kg/day (20 μg twice daily), with further adjustment depending on calcium levels. Six patients already displayed nephrocalcinosis. Severe side effects were reported in one child: two episodes of symptomatic hypocalcemia and one of iatrogenic hypercalcemia; one teenager displayed dysgueusia. Calcium levels at H0 did not significantly increase whilst calcium at H4 and phosphate levels significantly increased and decreased, respectively. After 12 months, eGFR, calcium and age-standardized phosphate levels were 108 (90–122) mL/min/1.73 m2, 2.36 (2.23–2.48) mmol/L, 0.5 (−0.1 to 1.5), and 68 (63–74) nmol/L, respectively, with a significant decrease in phosphate levels (p = 0.01). Urinary calcium and calcium/creatinine ratio remained stable; no nephrolithiasis was observed but two moderate nephrocalcinosis appeared.

Conclusion: Intermittent teriparatide therapy significantly improves calcium and phosphate control, without increasing calciuria. It appears to be safe and well-tolerated in children.
Oracle Recognition of Oracle Network Based on Ant Colony Algorithm
Authors: Shi Xianjin , Shen Xiajiong
Published: November 2021

Recent studies have shown that compared with traditional social networks, networks in which users socialize through interest recommendation have obvious homogeneity characteristics. Recommending topics of interest to users has become one of the main objectives of recommendation systems in such social networks, and the widespread data sparsity in such social networks has become the main problem faced by such recommendation systems. Particularly, in the oracle interest network, this problem is more difficult to solve because there are very few people who read and understand the Oracle. To address this problem, we propose an ant colony algorithm based recognition algorithm that can greatly expand the data in the oracle interest network and thus improve the efficiency of oracle interest network recommendation in this paper. Using the one-to-one correspondence between characters and translation in Oracle rubbings, the Oracle recognition problem is transformed into character matching problem, which can skip manual feature engineering experts, so as to realize efficient Oracle recognition. First, the coordinates of each character in the oracle bones are extracted. Then, the matching degree value of each oracle character corresponding to the translation of the oracle rubbings is assigned according to the coordinates. Finally, the maximum matching degree value of each character is searched using the improved ant colony algorithm, and the search result is the Chinese character corresponding to the oracle rubbings. In this paper, through experimental simulation, it is proved that this method is very effective when applied to the field of oracle recognition, and the recognition rate can approach 100% in some special oracle rubbings.
Multimodal Art Pose Recognition and Interaction With Human Intelligence Enhancement
Authors: Ma Chengming , Liu Qian , Dang Yaqi
Published: November 2021

This paper provides an in-depth study and analysis of human artistic poses through intelligently enhanced multimodal artistic pose recognition. A complementary network model architecture of multimodal information based on motion energy proposed. The network exploits both the rich information of appearance features provided by RGB data and the depth information provided by depth data as well as the characteristics of robustness to luminance and observation angle. The multimodal fusion is accomplished by the complementary information characteristics of the two modalities. Moreover, to better model the long-range temporal structure while considering action classes with sub-action sharing phenomena, an energy-guided video segmentation method is employed. And in the feature fusion stage, a cross-modal cross-fusion approach is proposed, which enables the convolutional network to share local features of two modalities not only in the shallow layer but also to obtain the fusion of global features in the deep convolutional layer by connecting the feature maps of multiple convolutional layers. Firstly, the Kinect camera is used to acquire the color image data of the human body, the depth image data, and the 3D coordinate data of the skeletal points using the Open pose open-source framework. Then, the action automatically extracted from keyframes based on the distance between the hand and the head, and the relative distance features are extracted from the keyframes to describe the action, the local occupancy pattern features and HSV color space features are extracted to describe the object, and finally, the feature fusion is performed and the complex action recognition task is completed. To solve the consistency problem of virtual-reality fusion, the mapping relationship between hand joint point coordinates and the virtual scene is determined in the augmented reality scene, and the coordinate consistency model of natural hand and virtual model is established; finally, the real-time interaction between hand gesture and virtual model is realized, and the average correct rate of its hand gesture reaches 99.04%, which improves the robustness and real-time interaction of hand gesture recognition.
Exploring the Experience of Healthcare Workers Who Returned to Work After Recovering From COVID-19: A Qualitative Study
Authors: Zhang Hui , Chen Dandan , Zou Ping , Cui Nianqi , Shao Jing , Qiu Ruoling , Wang Xiyi , Wu Man , Zhao Yi
Published: November 2021

Background: To date, a large body of literature focuses on the experience of healthcare providers who cared for COVID-19 patients. Qualitative studies exploring the experience of healthcare workers in the workplace after recovering from COVID-19 are limited. This study aimed to describe the experience of healthcare workers who returned to work after recovering from COVID-19.

Methods: This study employed a qualitative descriptive approach with a constructionist epistemology. Data were collected through semi-structured in-depth interviews with 20 nurses and physicians, and thematic analysis was used to identify themes from the interview transcripts.

Results: Three major themes about the psychological experiences of healthcare workers who had recovered from COVID-19 and returned to work were identified: (1) holding multi-faceted attitudes toward the career (sub-themes: increased professional identity, changing relationships between nurses, patients, and physicians, and drawing new boundaries between work and family), (2) struggling at work (sub-themes: poor interpersonal relationships due to COVID-19 stigma, emotional symptom burden, physical symptom burden, and workplace accommodations), (3) striving to return to normality (sub-themes: deliberate detachment, different forms of social support in the workplace, and long-term care from organizations).

Conclusions: The findings have highlighted opportunities and the necessity to promote health for this population. Programs centered around support, care, and stress management should be developed by policymakers and organizations. By doing this, healthcare workers would be better equipped to face ongoing crises as COVID-19 continues.
Phytoecdysteroids Accelerate Recovery of Skeletal Muscle Function Following in vivo Eccentric Contraction-Induced Injury in Adult and Old Mice
Authors: Zwetsloot Kevin A. , Shanely R. Andrew , Godwin Joshua S. , Hodgman Charles F.
Published: November 2021

Background: Eccentric muscle contractions are commonly used in exercise regimens, as well as in rehabilitation as a treatment against muscle atrophy and weakness. If repeated multiple times, eccentric contractions may result in skeletal muscle injury and loss of function. Skeletal muscle possesses the remarkable ability to repair and regenerate after an injury or damage; however, this ability is impaired with aging. Phytoecdysteroids are natural plant steroids that possess medicinal, pharmacological, and biological properties, with no adverse side effects in mammals. Previous research has demonstrated that administration of phytoecdysteroids, such as 20-hydroxyecdysone (20E), leads to an increase in protein synthesis signaling and skeletal muscle strength.

Methods: To investigate whether 20E enhances skeletal muscle recovery from eccentric contraction-induced damage, adult (7–8 mo) and old (26–27 mo) mice were subjected to injurious eccentric contractions (EC), followed by 20E or placebo (PLA) supplementation for 7 days. Contractile function via torque-frequency relationships (TF) was measured three times in each mouse: pre- and post-EC, as well as after the 7-day recovery period. Mice were anesthetized with isoflurane and then electrically-stimulated isometric contractions were performed to obtain in vivo muscle function of the anterior crural muscle group before injury (pre), followed by 150 EC, and then again post-injury (post). Following recovery from anesthesia, mice received either 20E (50 mg•kg−1 BW) or PLA by oral gavage. Mice were gavaged daily for 6 days and on day 7, the TF relationship was reassessed (7-day).

Results: EC resulted in significant reductions of muscle function post-injury, regardless of age or treatment condition (p < 0.001). 20E supplementation completely recovered muscle function after 7 days in both adult and old mice (pre vs. 7-day; p > 0.05), while PLA muscle function remained reduced (pre vs. 7-day; p < 0.01). In addition, histological markers of muscle damage appear lower in damaged muscle from 20E-treated mice after the 7-day recovery period, compared to PLA.

Conclusions: Taken together, these findings demonstrate that 20E fully recovers skeletal muscle function in both adult and old mice just 7 days after eccentric contraction-induced damage. However, the underlying mechanics by which 20E contributes to the accelerated recovery from muscle damage warrant further investigation.
The GoodHope Exercise and Rehabilitation (GEAR) Program for People With Ehlers-Danlos Syndromes and Generalized Hypermobility Spectrum Disorders
Authors: Mittal Nimish , Santa Mina Daniel , Buryk-Iggers Stephanie , Lopez-Hernandez Laura , Hussey Laura , Franzese Alyssa , Katz Joel , Laflamme Camille , McGillis Laura , McLean Lianne , Rachinsky Maxim , Rozenberg Dmitry , Slepian Maxwell , Weinrib Aliza , Clarke Hance
Published: November 2021

Introduction: The Ehlers-Danlos Syndromes (EDS) and Generalized Hypermobility Spectrum Disorders (G-HSD) comprise a heterogeneous group of genetic disorders of abnormal synthesis and/or maturation of collagen and other matricellular proteins. EDS is commonly characterized by manifestations such as multi joint hypermobility that can lead to musculoskeletal pains, subluxations and dislocations, fragile skin, organ dysfunction, and chronic significant diffuse pain with fatigue, deconditioning eventuating to poor quality of life. Evidence suggests exercise and rehabilitation interventions may ameliorate symptoms of unstable joints, recurrent subluxations/dislocations, and chronic widespread musculoskeletal pain. To date, there have only been a few reports describing exercise and rehabilitation care strategies for people with EDS.

Methods: In this manuscript, we describe the GoodHope Exercise and Rehabilitation (GEAR) program, its overarching principles, as well as the program development and delivery model. The GEAR program aims to decrease functional impairment, reduce pain, increase confidence in symptom self-management, and provide a community of support for people with EDS/G-HSD. To achieve these goals, we detail the model of care that includes exercise and rehabilitation therapy, education for self-management, and support accessing relevant community resources.

Strengths and Limitations of the Study: GEAR represents a novel exercise and rehabilitation care model for people with G-HSD and various clinical EDS subtypes, beyond the commonly included hEDS subtype. Systematic collection of data via validated measurements is ongoing and will guide the refinement of GEAR and support the development of emerging exercise and rehabilitation programs for people with EDS.
Analysis of Ionizing Radiation Induced DNA Damage by Superresolution dSTORM Microscopy
Authors: Brunner Szilvia , Varga Dániel , Bozó Renáta , Polanek Róbert , Tőkés Tünde , Szabó Emília Rita , Molnár Réka , Gémes Nikolett , Szebeni Gábor J. , Puskás László G. , Erdélyi Miklós , Hideghéty Katalin
Published: November 2021

The quantitative detection of radiation caused DNA double-strand breaks (DSB) by immunostained γ-H2AX foci using direct stochastic optical reconstruction microscopy (dSTORM) provides a deeper insight into the DNA repair process at nanoscale in a time-dependent manner. Glioblastoma (U251) cells were irradiated with 250 keV X-ray at 0, 2, 5, 8 Gy dose levels. Cell cycle phase distribution and apoptosis of U251 cells upon irradiation was assayed by flow cytometry. We studied the density, topology and volume of the γ-H2AX foci with 3D confocal microscopy and the dSTORM superresolution method. A pronounced increase in γ-H2AX foci and cluster density was detected by 3D confocal microscopy after 2 Gy, at 30 min postirradiation, but both returned to the control level at 24 h. Meanwhile, at 24 h a considerable amount of residual foci could be measured from 5 Gy, which returned to the normal level 48 h later. The dSTORM based γ-H2AX analysis revealed that the micron-sized γ-H2AX foci are composed of distinct smaller units with a few tens of nanometers. The density of these clusters, the epitope number and the dynamics of γ-H2AX foci loss could be analyzed. Our findings suggest a discrete level of repair enzyme capacity and the restart of the repair process for the residual DSBs, even beyond 24 h. The dSTORM superresolution technique provides a higher precision over 3D confocal microscopy to study radiation induced γ-H2AX foci and molecular rearrangements during the repair process, opening a novel perspective for radiation research.
Short-Term Decomposition and Nutrient-Supplying Ability of Sewage Sludge Digestate, Digestate Compost, and Vermicompost on Acidic Sandy and Calcareous Loamy Soils
Authors: Uzinger Nikolett , Szécsy Orsolya , Szűcs-Vásárhelyi Nóra , Padra István , Sándor Dániel Benjámin , Lončarić Zdenko , Draskovits Eszter , Rékási Márk
Published: November 2021

Organic waste and the compost and vermicompost derived from it may have different agronomic values, but little work is available on this aspect of sewage sludge. A 75-day pot experiment with perennial ryegrass (Lolium perenne) as the test plant aimed to investigate the fertiliser value and organic matter replenishment capacity of digested sewage sludge (DS) and the compost (COM) and vermicompost (VC) made from it, applied in 1% and 3% doses on acidic sand and calcareous loam. The NPK content and availability, changes in organic carbon content and plant biomass, and the efficiency of the amendments as nitrogen fertilisers were investigated. The final average residual carbon content for DS, COM, and VC was 35 ± 34, 85 ± 46, and 55 ± 46%, respectively. The organic carbon mineralisation rate depended on the soil type. The additives induced significant N mineralisation in both soils: the average increment in mineral N content was 1.7 times the total added N on acidic sand and 4.2 times it on calcareous loam for the 1% dose. The agronomic efficiency of COM and VC as fertilisers was lower than that of DS. In the short term, DS proved to be the best fertiliser, while COM was the best for organic matter replenishment.
Effect of Oxygen and Carbon Dioxide Concentration on the Quality of Minikiwi Fruits after Storage
Authors: Krupa Tomasz , Tomala Kazimierz
Published: November 2021

The rapid increase in the production of hardy kiwi fruit (A. arguta) since the beginning of the 21st century has required the development of new cultivation technologies and postharvest handling procedures in order to extend the supply and transport of the fruit to distant markets. Fruit storage focuses on the inhibition of ripening processes regulated by ethylene activity or respiration. Both of these are effectively regulated by appropriate concentrations of O2 and CO2 in the atmosphere surrounding the fruit. In this study, the effect of the concentration of both gases in the cold room on the physico-chemical indices of fruit quality, i.e., mass loss, firmness, soluble solids and monosaccharides content, titratable acidity and acid content, and color of the peel was evaluated. Studies have shown that high CO2 concentrations inhibit ripening processes more effectively than low O2 concentrations. Softening of berries as well as an increase in soluble solid contents was recorded during the first 4 weeks of storage in the fruit. However, the increase in monosaccharides was fairly stable throughout the study period. The increase in soluble solids content as well as the loss of acidity were more strongly determined by CO2 than O2, although the acid content in a 10% CO2 atmosphere did not change. Additionally, the fruits were greener after storage in 10% CO2, but the weakness was skin dulling and darkening. The results indicate that the use of high CO2 concentrations (5–10%) effectively inhibits ripening processes in fruit. After 12 weeks of storage, the fruit was still not suitable for direct consumption, which suggests that the storage period can be extended further.
The Emerging Role of Metabolism in Brain-Heart Axis: New Challenge for the Therapy and Prevention of Alzheimer Disease. May Thioredoxin Interacting Protein (TXNIP) Play a Role?
Authors: Perrone Lorena , Valente Mariarosaria
Published: November 2021

Alzheimer disease (AD) is the most frequent cause of dementia and up to now there is not an effective therapy to cure AD. In addition, AD onset occurs decades before the diagnosis, affecting the possibility to set up appropriate therapeutic strategies. For this reason, it is necessary to investigate the effects of risk factors, such as cardiovascular diseases, in promoting AD. AD shows not only brain dysfunction, but also alterations in peripheral tissues/organs. Indeed, it exists a reciprocal connection between brain and heart, where cardiovascular alterations participate to AD as well as AD seem to promote cardiovascular dysfunction. In addition, metabolic dysfunction promotes both cardiovascular diseases and AD. In this review, we summarize the pathways involved in the regulation of the brain-heart axis and the effect of metabolism on these pathways. We also present the studies showing the role of the gut microbiota on the brain-heart axis. Herein, we propose recent evidences of the function of Thioredoxin Interacting protein (TXNIP) in mediating the role of metabolism on the brain-heart axis. TXNIP is a key regulator of metabolism at both cellular and body level and it exerts also a pathological function in several cardiovascular diseases as well as in AD.
Integrated N- and O-Glycomics of Acute Myeloid Leukemia (AML) Cell Lines
Authors: Blöchl Constantin , Wang Di , Madunić Katarina , Lageveen-Kammeijer Guinevere S. M. , Huber Christian G. , Wuhrer Manfred , Zhang Tao
Published: November 2021

Acute myeloid leukemia (AML) is characterized by a dysregulated expansion of poorly differentiated myeloid cells. Although patients are usually treated effectively by chemotherapy, a high rate of relapsed or refractory disease poses a major hurdle in its treatment. Recently, several studies have proposed implications of protein glycosylation in the pathobiology of AML including chemoresistance. Accordingly, associations have been found between specific glycan epitopes and the outcome of the disease. To advance this poorly studied field, we performed an exploratory glycomics study characterizing 21 widely used AML cell lines. Exploiting the benefits of porous graphitized carbon chromatography coupled to tandem mass spectrometry (PGC nano-LC-MS2), we qualitatively and quantitatively profiled N- and O-linked glycans. AML cell lines exhibited distinct glycan fingerprints differing in relevant glycan traits correlating with their cellular phenotype as classified by the FAB system. By implementing transcriptomics data, specific glycosyltransferases and hematopoietic transcription factors were identified, which are candidate drivers of the glycan phenotype of these cells. In conclusion, we report the varying expression of glycan structures across a high number of AML cell lines, including those associated with poor prognosis, identified underlying glycosyltransferases and transcription factors, and provide insights into the regulation of the AML glycan repertoire.
PI3K Pathway Inhibition with NVP-BEZ235 Hinders Glycolytic Metabolism in Glioblastoma Multiforme Cells
Authors: Udawant Shreya , Litif Carl , Lopez Alma , Gunn Bonnie , Schuenzel Erin , Keniry Megan
Published: November 2021

Glioblastoma (GBM) is the most lethal primary brain cancer that lacks effective molecular targeted therapies. The PI3K/AKT/mTOR pathway is activated in 90% of all Glioblastoma multiforme (GBM) tumors. To gain insight into the impact of the PI3K pathway on GBM metabolism, we treated U87MG GBM cells with NVP-BEZ235 (PI3K and mTOR a dual inhibitor) and identified differentially expressed genes with RNA-seq analysis. RNA-seq identified 7803 differentially regulated genes in response to NVP-BEZ235. Gene Set Enrichment Analysis (GSEA) identified two glycolysis-related gene sets that were significantly enriched (p < 0.05) in control samples compared to NVP-BEZ235-treated samples. We validated the inhibition of glycolytic genes by NVP-BEZ235 and examined the impact of the FOXO1 inhibitor (AS1842856) on these genes in a set of GBM cell lines. FOXO1 inhibition alone was associated with reduced LDHA expression, but not ENO1 or PKM2. Bioinformatics analyses revealed that PI3K-impacted glycolytic genes were over-expressed and co-expressed in GBM clinical samples. The elevated expression of PI3K-impacted glycolytic genes was associated with poor prognosis in GBM based on Kaplan–Meier survival analyses. Our results suggest novel insights into hallmark metabolic reprogramming associated with the PI3K-mTOR dual inhibition.
Probing Electron Properties in ECR Plasmas Using X-ray Bremsstrahlung and Fluorescence Emission
Authors: Mishra Bharat , Pidatella Angelo , Galatà Alessio , Biri Sandor , Rácz Richard , Naselli Eugenia , Mazzaglia Maria , Torrisi Giuseppe , Mascali David
Published: November 2021

A quantitative analysis of X-ray emission from an electron cyclotron resonance (ECR) plasma was performed to probe the spatial properties of electrons having energy for effective ionisation. A series of measurements were taken by INFN-LNS and ATOMKI, capturing spatially and spectrally resolved X-ray maps as well as volumetric emissions from argon plasma. Comparing the former with model generated maps (involving space-resolved phenomenological electron energy distribution function and geometrical efficiency calculated using ray-tracing Monte Carlo (MC) routine) furnished information on structural aspects of the plasma. Similarly, fitting a model composed of bremsstrahlung and fluorescence to the volumetric X-ray spectrum provided valuable insight into the density and temperature of confined and lost electrons. The latter can be fed back to existing electron kinetics models for simulating more relevant energies, consequently improving theoretical X-ray maps and establishing the method as an excellent indirect diagnostic tool for warm electrons, required for both fundamental and applied research in ECR plasmas.
Joint UAVs’ Load Balancing and UEs’ Data Rate Fairness Optimization by Diffusion UAV Deployment Algorithm in Multi-UAV Networks
Authors: Luan Zhirong , Jia Hongtao , Wang Ping , Jia Rong , Chen Badong
Published: November 2021

Unmanned aerial vehicles (UAVs) can be deployed as base stations (BSs) for emergency communications of user equipments (UEs) in 5G/6G networks. In multi-UAV communication networks, UAVs’ load balancing and UEs’ data rate fairness are two challenging problems and can be optimized by UAV deployment strategies. In this work, we found that these two problems are related by the same performance metric, which makes it possible to optimize the two problems simultaneously. To solve this joint optimization problem, we propose a UAV diffusion deployment algorithm based on the virtual force field method. Firstly, according to the unique performance metric, we define two new virtual forces, which are the UAV-UAV force and UE-UAV force defined by FU and FV, respectively. FV is the main contributor to load balancing and UEs’ data rate fairness, and FU contributes to fine tuning the UEs’ data rate fairness performance. Secondly, we propose a diffusion control stratedy to the update UAV-UAV force, which optimizes FV in a distributed manner. In this diffusion strategy, each UAV optimizes the local parameter by exchanging information with neighbor UAVs, which achieve global load balancing in a distributed manner. Thirdly, we adopt the successive convex optimization method to update FU, which is a non-convex problem. The resultant force of FV and FU is used to control the UAVs’ motion. Simulation results show that the proposed algorithm outperforms the baseline algorithm on UAVs’ load balancing and UEs’ data rate fairness.
Lifecycle Assessment of a Non-Phase-Transition Drying Pyrolysis and Mass Conversion Technology
Authors: Gao Yulei , Jiang Jikang , Shen Zheng , Zhang Xu , Zeng Lingjie , Shao Xiaolu
Published: November 2021

A lifecycle model was established to explore the efficiency, economy, and greenhouse gas emissions of a non-phase-transition drying pyrolysis and mass conversion technology, based on the principle of lifecycle assessment. The evaluation scope included straw collection and transportation, drying and crushing, biomass pyrolysis, charcoal processing, and waste heat utilization. The results show that the energy output/input ratio for non-phase-transition drying pyrolysis was 20.43, and the energy efficiency was high. The pure profit from treating wet straw was USD 45.32 per ton, the profit margin of sales was 52.11%, and the economic benefit was high. The equivalent emission of CO2 was 34.10 g·MJ−1, demonstrating high environmental benefits. Therefore, non-phase-transition drying pyrolysis and mass conversion technology is a potential biomass utilization technology with energy, economic, and ecological benefits.
The Influence of Investors’ Mood on the Stock Prices: Evidence from Energy Firms in Warsaw Stock Exchange, Poland
Authors: Tarczyński Waldemar , Mentel Urszula , Mentel Grzegorz , Shahzad Umer
Published: November 2021

The subject of this publication is an analysis of the sentiment of stock exchange investors in terms of making investment decisions in the energy sector of the Polish stock exchange. The investment mood is considered in the context of the possible impact of weather factors on investment decisions. Possible effects are verified in relation to the rates of return and the volume of trading of energy sector entities. The analysis is carried out both in terms of co-integration analyses as well as in econometric terms, in the cross-section of classic OLS models or causality analysis using VAR vector autoregression models. The main purpose of the issues discussed is the problem of indicating (illustrating) the presence or absence of mutual relations between weather factors and the stock market in terms of the methods considered.