bluuebunny/crossref_metadata_2025 huggingface dataset:
Dimensional Analysis Model Predicting the Number of Food Microorganisms
Authors: Li Cuiqin , He Laping , Hu Yuedan , Liu Hanyu , Wang Xiao , Chen Li , Zeng Xuefeng
Published: February 2022

Predicting the number of microorganisms has excellent application in the food industry. It helps in predicting and managing the storage time and food safety. This study aimed to establish a new, simple, and effective model for predicting the number of microorganisms. The dimensional analysis model (DAM) was established based on dimensionless analysis and the Pi theorem. It was then applied to predict the number of Pseudomonas in Niuganba (NGB), a traditional Chinese fermented dry-cured beef, which was prepared and stored at 278 K, 283 K, and 288 K. Finally, the internal and external validation of the DAM was performed using six parameters including R2, R2adj, root mean square error (RMSE), standard error of prediction (%SEP), Af, and Bf. High R2 and R2adj and low RMSE and %SEP values indicated that the DAM had high accuracy in predicting the number of microorganisms and the storage time of NGB samples. Both Af and Bf values were close to 1. The correlation between the observed and predicted numbers of Pseudomonas was high. The study showed that the DAM was a simple, unified and effective model to predict the number of microorganisms and storage time.
The Emergence of Model Systems to Investigate the Link Between Traumatic Brain Injury and Alzheimer’s Disease
Authors: Srinivasan Gayathri , Brafman David A.
Published: February 2022

Numerous epidemiological studies have demonstrated that individuals who have sustained a traumatic brain injury (TBI) have an elevated risk for developing Alzheimer’s disease and Alzheimer’s-related dementias (AD/ADRD). Despite these connections, the underlying mechanisms by which TBI induces AD-related pathology, neuronal dysfunction, and cognitive decline have yet to be elucidated. In this review, we will discuss the various in vivo and in vitro models that are being employed to provide more definite mechanistic relationships between TBI-induced mechanical injury and AD-related phenotypes. In particular, we will highlight the strengths and weaknesses of each of these model systems as it relates to advancing the understanding of the mechanisms that lead to TBI-induced AD onset and progression as well as providing platforms to evaluate potential therapies. Finally, we will discuss how emerging methods including the use of human induced pluripotent stem cell (hiPSC)-derived cultures and genome engineering technologies can be employed to generate better models of TBI-induced AD.
mito-TEMPO Attenuates Oxidative Stress and Mitochondrial Dysfunction in Noise-Induced Hearing Loss via Maintaining TFAM-mtDNA Interaction and Mitochondrial Biogenesis
Authors: Chen Jia-Wei , Ma Peng-Wei , Yuan Hao , Wang Wei-Long , Lu Pei-Heng , Ding Xue-Rui , Lun Yu-Qiang , Yang Qian , Lu Lian-Jun
Published: February 2022

The excessive generation of reactive oxygen species (ROS) and mitochondrial damage have been widely reported in noise-induced hearing loss (NIHL). However, the specific mechanism of noise-induced mitochondrial damage remains largely unclear. In this study, we showed that acoustic trauma caused oxidative damage to mitochondrial DNA (mtDNA), leading to the reduction of mtDNA content, mitochondrial gene expression and ATP level in rat cochleae. The expression level and mtDNA-binding function of mitochondrial transcription factor A (TFAM) were impaired following acoustic trauma without affecting the upstream PGC-1α and NRF-1. The mitochondria-target antioxidant mito-TEMPO (MT) was demonstrated to enter the inner ear after the systemic administration. MT treatment significantly alleviated noise-induced auditory threshold shifts 3d and 14d after noise exposure. Furthermore, MT significantly reduced outer hair cell (OHC) loss, cochlear ribbon synapse loss, and auditory nerve fiber (ANF) degeneration after the noise exposure. In addition, we found that MT treatment effectively attenuated noise-induced cochlear oxidative stress and mtDNA damage, as indicated by DHE, 4-HNE, and 8-OHdG. MT treatment also improved mitochondrial biogenesis, ATP generation, and TFAM-mtDNA interaction in the cochlea. These findings suggest that MT has protective effects against NIHL via maintaining TFAM-mtDNA interaction and mitochondrial biogenesis based on its ROS scavenging capacity.
Multimodal Evoked Potentials as Potential Biomarkers of Disease Activity in Patients With Clinically Isolated Syndrome
Authors: Dziadkowiak Edyta , Wieczorek Małgorzata , Zagrajek Mieszko , Chojdak-Łukasiewicz Justyna , Gruszka Ewa , Budrewicz Sławomir , Pokryszko-Dragan Anna
Published: February 2022

Objective

There is an ongoing search for markers useful in monitoring and predicting disease activity at the early stage of multiple sclerosis (MS). The goals of this study were to prospectively evaluate the changes in parameters of multimodal evoked potentials (EP) and cognition within a 3-year follow-up period in patients with clinically isolated syndrome (CIS), and to assess the prognostic value of baseline findings with regard to the disease outcomes.

Methods

In 29 patients (20 women, nine men, mean age 31.1) multimodal (visual, brainstem auditory, somatosensory, event-related) EP and neuropsychological tests (NT) were performed at baseline (T0) and after 1 (T1) and 3 (T3) years. Their results were compared longitudinally between baseline, T1, and T3. Baseline results confirmed conversion of CIS into multiple sclerosis (MS) and disability level at T1 and T3 using multiple comparisons and a logistic regression model.

Results

Apart from mean N13/P16 SEP (somatosensory evoked potentials) amplitude (lower at T1 and T3 than at baseline (T0 1.02 ± 0.37 μV, T1 0.90 ± 0.26 μV, T3 0.74 ± 0.32 μV, p < 0.05 for both comparisons), no significant changes of EP or NT parameters were found in longitudinal assessment. Baseline P300 Pz latency was longer for the patients with MS than for those with CIS at T1 (352.69 vs. 325.56 ms). No predictive value was shown for any of the analyzed baseline variables with regard to conversion from CIS into MS.

Significance

Baseline ERP abnormalities were associated with their short-term conversion into MS. ERP are worth considering in multimodal EP evaluation at the early stage of MS.
Nomogram for Predicting Facial Nerve Outcomes After Surgical Resection of Vestibular Schwannoma
Authors: Sun Yang , Yang Jianhua , Li Tang , Gao Kaiming , Tong Xiaoguang
Published: February 2022

Objective

The facial nerve (FN) outcomes after vestibular schwannoma surgery seriously affect the social psychology and quality of life of patients. More and more attention has been paid to the protection of FN function. This study aimed to identify significant prognostic factors for FN outcomes after vestibular schwannoma surgery and create a new nomogram for predicting the rates of poor FN outcomes.

Methods

Data from patients who had undergone operations for vestibular schwannoma between 2015 and 2020 were retrieved retrospectively and patients were divided into good and poor FN outcomes groups according to postoperative nerve function. The nomogram for predicting the risk of poor FN outcomes was constructed from the results of the univariate logistic regression analysis and the multivariate logistic regression analysis of the influencing factors for FN outcomes after surgical resection of vestibular schwannoma.

Results

A total of 392 participants were enrolled. The univariate logistic regression analysis revealed that age, tumor size, cystic features of tumors, cerebrospinal fluid (CSF) cleft sign, tumor adhesion to the nerve, learning curve, and FN position were statistically significant. The multivariate logistic regression analysis showed that age, tumor size, cystic features of tumors, CSF cleft sign, tumor adhesion to the nerve, learning curve, and FN position were independent factors. The nomogram model was constructed according to these indicators. At the last follow-up examination, a good FN outcome was observed in 342 patients (87.2%) and only 50 patients (12.8%) was presented with poor FN function. Application of the nomogram in the validation cohort still gave good discrimination [area under the curve (AUC), 0.806 (95% CI, 0.752–0.861)] and good calibration.

Conclusion

This study has presented a reliable and valuable nomogram that can accurately predict the occurrence of poor FN outcomes after surgery in patients. This tool is easy to use and could assist doctors in establishing clinical decision-making for individual patients.
Effectiveness of Standard-Dose vs. Low-Dose Alteplase for Acute Ischemic Stroke Within 3–4.5 h
Authors: Chen Chih-Hao , Tang Sung-Chun , Chen Yu-Wei , Chen Chih-Hung , Tsai Li-Kai , Sung Sheng-Feng , Lin Huey-Juan , Huang Hung-Yu , Po Helen L. , Sun Yu , Chen Po-Lin , Chan Lung , Wei Cheng-Yu , Lee Jiunn-Tay , Hsieh Cheng-Yang , Lin Yung-Yang , Lien Li-Ming , Jeng Jiann-Shing
Published: February 2022

Background

The efficacy and safety of intravenous alteplase administered 3–4.5 h after acute ischemic stroke have been demonstrated. However, whether responses differ between low-dose and standard-dose alteplase during this time window and whether certain subgroups benefit more remain unknown.

Patients and Methods

The current analysis was based on a multicenter matched-cohort study conducted in Taiwan. The treatment group comprised 378 patients receiving intravenous alteplase 3–4.5 h after stroke onset, and the control group comprised 378 age- and sex-matched patients who did not receive alteplase treatment during the same period. Standard- and low-dose alteplase was administered to patients at the physician's discretion.

Results

Overall, patients receiving alteplase exhibited more favorable outcomes than did controls [34.0 vs. 22.7%; odds ratio (OR): 1.75, 95% confidence interval (CI): 1.27–1.42], and the effectiveness was consistent in all subgroups. Although patients in the standard-dose group (n = 182) were younger than those in the low-dose (n = 192) group, the proportions of patients with favorable outcomes (36.3 vs. 31.8%; OR: 1.22, 95% CI: 0.80–1.88) and symptomatic hemorrhage (2.8 vs 4.2%; OR: 0.65, 95% CI: 0.21–2.02) were consistently comparable in a covariate-adjusted model and an age-matched cohort. In the subgroup analysis, patients with cardioembolism, atrial fibrillation, and hypercholesterolemia were more likely to achieve favorable outcomes after receiving standard-dose than low-dose alteplase.

Conclusion

In the 3–4.5 h time window, the effectiveness and safety of standard-dose and low-dose alteplase may be comparable. A standard dose may be selected for patients with cardioembolism, atrial fibrillation, or hypercholesterolemia.
Natural History Studies in NCL and Their Expanding Role in Drug Development: Experiences From CLN2 Disease and Relevance for Clinical Trials
Authors: Nickel Miriam , Schulz Angela
Published: February 2022

Conducting clinical trials in rare diseases is challenging. In trials that aim to use natural history control cohorts for evaluation of efficacy, lack of data on natural history of disease prolongs development of future therapies significantly. Therefore, collection of valid natural history data in clinical settings is needed to advance drug development. These data need to fulfill requirements on type of collection, quantifiable measures on the course of disease, verification and monitoring as well as compliance to strict data protection and sharing policies. Disease registries can be a source for patient data. Late-infantile CLN2 disease is characterized by rapid psychomotor decline and epilepsy. Natural-history data of 140 genotype-confirmed CLN2 patients from two independent, international cohorts were analyzed in a natural history study. Both datasets included quantitative ratings with disease-specific clinical scores. Among 41 patients for whom longitudinal assessments spanning an extended disease course were available within the DEM-CHILD DB (an international NCL disease patient database, NCT04613089), a rapid loss of motor and language abilities was documented in quantitative detail. Data showed that the course of disease in late-infantile CLN2 disease is highly predictable with regard to the loss of language and motor function and that the results were homogeneous across multiple and international sites. These data were accepted by EMA and FDA as valid natural-history controls for the evaluation of efficacy in experimental therapies for CLN2 disease and led to an expedited approval of intracerebroventricular enzyme replacement therapy with cerliponase alpha in May 2017.
The Differentiation of Self-Motion From External Motion Is a Prerequisite for Postural Control: A Narrative Review of Visual-Vestibular Interaction
Authors: Chaudhary Shikha , Saywell Nicola , Taylor Denise
Published: February 2022

The visual system is a source of sensory information that perceives environmental stimuli and interacts with other sensory systems to generate visual and postural responses to maintain postural stability. Although the three sensory systems; the visual, vestibular, and somatosensory systems work concurrently to maintain postural control, the visual and vestibular system interaction is vital to differentiate self-motion from external motion to maintain postural stability. The visual system influences postural control playing a key role in perceiving information required for this differentiation. The visual system’s main afferent information consists of optic flow and retinal slip that lead to the generation of visual and postural responses. Visual fixations generated by the visual system interact with the afferent information and the vestibular system to maintain visual and postural stability. This review synthesizes the roles of the visual system and their interaction with the vestibular system, to maintain postural stability.
Social Disparities of Pain and Pain Intensity Among Women Diagnosed With Early Stage Breast Cancer
Authors: Choi Hyo Young , Graetz Ilana , Shaban-Nejad Arash , Schwartzberg Lee , Vidal Gregory , Davis Robert Lowell , Shin Eun Kyong
Published: February 2022

Background

Breast cancer is one of the most commonly diagnosed cancers among women in the United States and pain is the most common side effect of breast cancer and its treatment. Yet, the relationships between social determinants of pain and pain experience/intensity remain under-investigated. We examined the associations between social determinants of pain both at the individual level and the neighborhood level to understand how social conditions are associated with pain perception among early stage breast cancer patients.

Methods

We conducted integrated statistical analysis of 1,191 women with early stage breast cancer treated at a large cancer center in Memphis, Tennessee. Combining electronic health records, patient-reported data and census data regarding residential address at the time of first diagnosis, we evaluated the relationships between social determinants and pain perception. Pain responses were self-reported by a patient as a numerical rating scale score at the patient’s initial diagnosis and follow-up clinical visits. We implemented two sets of statistical analyses of the zero-inflated Poisson model and estimated the associations between neighborhood poverty prevalence and breast cancer pain intensity. After adjustment for demographic characteristics, cancer stage, and chemotherapy, pain perception was significantly associated with poverty and blight level of the neighborhood.

Results

Among women living in the highest-poverty areas, the odds of reporting pain were 2.48 times higher than those in the lowest-poverty area. Women living in the highest-blight area had 5.43 times higher odds of reporting pain than those in the lowest-blight area. Neighborhood-level social determinants were significantly associated with pain intensity among women diagnosed with early-stage breast cancer.

Conclusions

Distressed neighborhood conditions are significantly associated with higher pain perception. Breast cancer patients living in socio-economically disadvantaged neighborhoods and in poor environmental conditions reported higher pain severity compared to patients from less distressed neighborhoods. Therefore, post-diagnosis pain treatment design needs to be tailored to the social determinants of the breast cancer patients.
What Clonal Hematopoiesis Can Teach Us About MDS
Authors: Chan Irenaeus C. C. , Wiley Brian J. , Bolton Kelly L.
Published: February 2022

Clonal hematopoiesis (CH), defined as the clonal expansion of mutated hematopoietic stem and progenitor cells (HSPCs), is a common aging process. CH is a risk factor for the development of hematologic malignancies, most commonly myeloid neoplasms (MNs) including acute myeloid leukemia (AML), myelodysplastic syndrome (MDS), and myeloproliferative neoplasm (MPN). Recent work has elucidated how the development and cellular fitness of CH is shaped by aging, environmental exposures, and the germline (inherited) genetic background of an individual. This in turn has provided valuable insights into the pathogenesis of MNs including MDS. Here, in this review, we discuss the genetic origins of CH, the environmental stressors that influence CH, and the implications of CH on health outcomes including MDS. Since MNs have shared risk factors and underlying biology, most of our discussion regarding the implications of CH surrounds MN in general rather than focusing specifically on MDS. We conclude with future directions and areas of investigation including how intervention studies of CH might inform future therapeutic approaches to MN including MDS.
Understanding Anthracycline Cardiotoxicity From Mitochondrial Aspect
Authors: Huang Junqi , Wu Rundong , Chen Linyi , Yang Ziqiang , Yan Daoguang , Li Mingchuan
Published: February 2022

Anthracyclines, such as doxorubicin, represent one group of chemotherapy drugs with the most cardiotoxicity. Despite that anthracyclines are capable of treating assorted solid tumors and hematological malignancies, the side effect of inducing cardiac dysfunction has hampered their clinical use. Currently, the mechanism underlying anthracycline cardiotoxicity remains obscure. Increasing evidence points to mitochondria, the energy factory of cardiomyocytes, as a major target of anthracyclines. In this review, we will summarize recent findings about mitochondrial mechanism during anthracycline cardiotoxicity. In particular, we will focus on the following aspects: 1) the traditional view about anthracycline-induced reactive oxygen species (ROS), which is produced by mitochondria, but in turn causes mitochondrial injury. 2) Mitochondrial iron-overload and ferroptosis during anthracycline cardiotoxicity. 3) Autophagy, mitophagy and mitochondrial dynamics during anthracycline cardiotoxicity. 4) Anthracycline-induced disruption of cardiac metabolism.
Asiatic Acid Attenuates Osteoporotic Bone Loss in Ovariectomized Mice Through Inhibiting NF-kappaB/MAPK/ Protein Kinase B Signaling Pathway
Authors: Dong Mingming , Zeng Jican , Yang Chenyu , Qiu Yisen , Wang Xinjia
Published: February 2022

Osteoporosis is a condition associated with osteolytic bone disease that is primarily characterized by inordinate osteoclast activation. Protein kinase B (Akt) pathways activated by receptor activator of nuclear factor kappa-B ligand (RANKL) are essential for osteoclastogenesis. Asiatic acid (AA) is a natural pentacyclic triterpenoid compound extracted from a traditional Chinese herb that exhibits a wide range of biological activities. AA has been found to alleviate the hypertrophic and fibrotic phenotype of chondrocytes via the Akt signaling pathway. In this study, we investigated whether AA alleviated bone loss by inhibiting the Akt signaling pathway during osteoclastogenesis and its effect on osteoblasts. The effect of AA cytotoxicity on mouse bone marrow-derived macrophages/monocytes (BMMs) was evaluated in vitro using a Cell Counting Kit-8 assay. The effects of AA on osteoclast differentiation and function were detected using tartrate-resistant acid phosphatase (TRAP) staining and a pit formation assay. A Western blot and qRT-PCR were conducted to evaluate the expression of osteoclast-specific genes and protein signaling molecules. In addition, alkaline phosphatase and alizarin red staining were performed to assess osteoblast differentiation and mineralization. The bone protective effect of AA was investigated in vivo using ovariectomized mice. we found that AA could dose-dependently inhibit RANKL-induced osteoclastogenesis. Moreover, the pit formation assay revealed that osteoclast function was suppressed by treatment with AA. Moreover, the expression of osteoclast-specific genes was found to be substantially decreased during osteoclastogenesis. Analysis of the molecular mechanisms showed that AA could inhibit NF-kappaB/MAPK/Akt signaling pathway, as well as the downstream factors of NFATc1 in the osteoclast signaling pathway activated by RANKL. However, AA did not significantly promote osteoblast differentiation and mineralization. The in vivo experiments suggested that AA could alleviate ovariectomy-induced bone loss in ovariectomized mice. Our results demonstrate that AA can inhibit osteoclastogenesis and prevent ovariectomy-induced bone loss by inhibiting the NF-kappaB/MAPK/Akt signaling pathway. The discovery of the new molecular mechanism that AA inhibits osteoclastogenesis provides essential evidence to support the use of AA as a potential drug for the treatment of osteoclast-related diseases.
Impact of Combining Long-Term Subsoiling and Organic Fertilizer on Soil Microbial Biomass Carbon and Nitrogen, Soil Enzyme Activity, and Water Use of Winter Wheat
Authors: Yang Yonghui , Li Minjie , Wu Jicheng , Pan Xiaoying , Gao Cuimin , Tang Darrell W. S.
Published: February 2022

Reductions in soil productivity and soil water retention capacity, and water scarcity during crop growth, may occur due to long-term suboptimal tillage and fertilization practices. Therefore, the application of appropriate tillage (subsoiling) and fertilization (organic fertilizer) practices is important for improving soil structure, water conservation and soil productivity. We hypothesize that subsoiling tillage combined with organic fertilizer has a better effect than subsoiling or organic fertilizer alone. A field experiment in Henan, China, has been conducted since 2011 to explore the effects of subsoiling and organic fertilizer, in combination, on winter wheat (Triticum aestivum L.) farming. We studied the effects of conventional tillage (CT), subsoiling (S), organic fertilizer (OF), and organic fertilizer combined with subsoiling (S+OF) treatments on dry matter accumulation (DM), water consumption (ET), water use efficiency (WUE) at different growth stages, yield, and water production efficiency (WPE) of winter wheat over 3 years (2016–2017, 2017–2018, 2018–2019). We also analyzed the soil structure, soil organic carbon, soil microbial biomass carbon and nitrogen, and soil enzymes in 2019. The results indicate that compared with CT, the S, OF and S+OF treatments increased the proportion of >0.25 mm aggregates, and S+OF especially led to increased soil organic carbon, soil microbial biomass carbon and nitrogen, soil enzyme activity (sucrase, cellulose, and urease). S+OF treatment was most effective in reducing ET, and increasing DM and WUE during the entire growth period of wheat. S+OF treatment also increased the total dry matter accumulation (Total DM) and total water use efficiency (total WUE) by 18.6–32.0% and 36.6–42.7%, respectively, during these 3 years. Wheat yield and WPE under S+OF treatment increased by 11.6–28.6% and 26.8–43.6%, respectively, in these 3 years. Therefore, S+OF in combination was found to be superior to S or OF alone, which in turn yielded better results than the CT.
Quantitative Trait Loci Mapping for Earliness, Fruit, and Seed Related Traits Using High Density Genotyping-by-Sequencing-Based Genetic Map in Bitter Gourd (Momordica charantia L.)
Authors: Kaur Gurpreet , Pathak Mamta , Singla Deepak , Chhabra Gautam , Chhuneja Parveen , Kaur Sarao Navraj
Published: February 2022

Bitter gourd (Momordica charantia L.) is an important vegetable crop having numerous medicinal properties. Earliness and yield related traits are main aims of bitter gourd breeding program. High resolution quantitative trait loci (QTLs) mapping can help in understanding the molecular basis of phenotypic variation of these traits and thus facilitate marker-assisted breeding. The aim of present study was to identify genetic loci controlling earliness, fruit, and seed related traits. To achieve this, genotyping-by-sequencing (GBS) approach was used to genotype 101 individuals of F4 population derived from a cross between an elite cultivar Punjab-14 and PAUBG-6. This population was phenotyped under net-house conditions for three years 2018, 2019, and 2021. The linkage map consisting of 15 linkage groups comprising 3,144 single nucleotide polymorphism (SNP) markers was used to detect the QTLs for nine traits. A total of 50 QTLs for these traits were detected which were distributed on 11 chromosomes. The QTLs explained 5.09–29.82% of the phenotypic variance. The highest logarithm of the odds (LOD) score for a single QTL was 8.68 and the lowest was 2.50. For the earliness related traits, a total of 22 QTLs were detected. For the fruit related traits, a total of 16 QTLs and for seed related traits, a total of 12 QTLs were detected. Out of 50 QTLs, 20 QTLs were considered as frequent QTLs (FQ-QTLs). The information generated in this study is very useful in the future for fine-mapping and marker-assisted selection for these traits in bitter gourd improvement program.
Socioemotional Exchanges Between Men and Women in Heterosexual Relationships
Authors: Gaines Stanley O. , Sedikides Constantine
Published: February 2022

We examined affection-giving, affection-denying, respect-giving, and respect-denying behaviors among men and women in heterosexual relationships. In a pilot study (N = 106 couples), although we had expected the latent variables of affectionate and respectful behaviors to emerge from exploratory factor analyses, we obtained the latent variables of socioemotional rewards and costs instead. In the main study (initial N = 182 couples), we replicated the factor patterns of socioemotional rewards and costs in confirmatory factor analyses. Moreover, we entered (final N = 177 couples) men’s and women’s self-reported narcissism alongside men’s and women’s socioemotional rewards and costs, as reported by partners, into a dyadic model that we tested via covariance structure analyses. Results revealed that, although men and women reciprocated rewards as well as costs (and correlations between individuals’ rewards and costs were negative), narcissism was not reflected in the patterns of reciprocity (men’s and women’s narcissism were positively related.) We discuss implications for studies of relationship processes as two-person group dynamics.
Effect of Digital Marketing Capabilities and Blockchain Technology on Organizational Performance and Psychology
Authors: Liu Ying
Published: February 2022

Digitalization plays an integral role in the transformation of the Omni structure. This study aims to investigate the effect of digital marketing capabilities (DMCs) and blockchain technology on customer-linking capabilities (CLCs), market-sensing capabilities (MSCs), consumer-brand engagement (CBE), and firm performance in China. The study was quantitative, and a simple random sampling technique was adopted for data collection. Data were collected using a structured questionnaire, 311 questionnaires were distributed, and a 5-point Likert scale was used to collect the data from the respondents who were employed in the Omni structure industries. The research hypothesis was tested using the structural equation modeling (SEM) technique. The results have identified a significant correlation and direct effect between DMCs, CLCs, MSCs, and firm performance. Remarkably, the effect of DMCs on CBE is significant. The mediating effect of MSCs and CLCs is significant between the relationship of DMCs and firm performance. The organization performance in the Omni structure depends on how well the DMCs have been employed. The DMCs influence MSCs, CLCs, and CBE. Henceforth, this study contributes by analyzing the role of DMCs in blockchain technology.
The Compulsory Care Act: Early Observations and Expectations of In- or Outpatient Involuntary Treatment
Authors: Gemsa Stephan , Noorthoorn Eric O. , Lepping Peter , de Haan Hein A. , Wierdsma Andre I. , Hutschemaekers Giel J. M.
Published: February 2022

Background

On January 1, 2020, the Dutch Compulsory Care Act (WvGGZ) replaced the Special Admissions Act (BOPZ). While the old law only allowed compulsory treatment in hospitals, the new law allows it both inside and outside the hospital. Moreover, the new law prioritizes the patient's own opinion on coercive measures. By following patients' own choices, the Compulsory Care Act is hoped to lead to fewer admission days and less inpatient compulsory treatment in involuntarily admitted patients.

Methods

We studied the seclusion and enforced-medication events before and after January 1, 2020, using coercive measures monitoring data in a Mental Health Trust. Trends in hours of seclusion and the number of enforced-medication events per month from 2012 to 2019 were compared with 2020. We used generalized linear models to perform time series analysis. Logistic regression analyses and generalized linear models were performed to investigate whether patient compilation determined some of the observed changes in seclusion use or enforced-medication events.

Results

The mean number of hours of seclusion between 2012 and 2019 was 27,124 per year, decreasing from 48,542 in 2012 to 21,133 in 2019 to 3,844 h in 2020. The mean incidence of enforced-medication events between 2012 and 2019 was 167, increasing from 90 in 2012 to 361 in 2019 and then fell to 294 in 2020. In 2020, we observed 3,844 h of seclusion and 294 enforced-medication events. Near to no outpatient coercion was reported, even though it was warranted. The time series analysis showed a significant effect of the year 2020 on seclusion hours (β = −1.867; Exp(β) = 0.155, Wald = 27.22, p = 0.001), but not on enforced-medication events [β = 0.48; Exp(β) = 1.616, Wald = 2.33, p = 0.13].

Discussion

There was a reduction in the number of seclusion hours after the introduction of the Compulsory Care Act. The number of enforced-medication events also increased from a very low baseline, but from 2017 onwards. To see whether these findings are consistent over time, they need to be replicated in the near future.

Conclusion

We observed a significant increase in enforced-medication use and a decrease in seclusion hours. The year 2020 predicted seclusion hours, but not enforced-medication events.
The Relationship Between Generalised Joint Hypermobility and Autism Spectrum Disorder in Adults: A Large, Cross-Sectional, Case Control Comparison
Authors: Glans Martin R. , Thelin Nils , Humble Mats B. , Elwin Marie , Bejerot Susanne
Published: February 2022

Autism spectrum disorder (ASD) and generalised joint hypermobility (GJH) share a number of clinical manifestations including proprioceptive impairment, motor difficulties, sensory hypersensitivity, and autonomic dysfunction. Clinical observations suggest that GJH is overrepresented in ASD. However, there are currently few systematic studies available. Knowledge about comorbidities may unfold common aetiopathological pathways underlying the association and improve the clinical management. The aim of this large, cross-sectional comparative study is to evaluate the relationship between ASD and GJH in adults. Data on joint hypermobility, symptoms associated with both hypermobility spectrum disorders (HSD) and hypermobile Ehlers-Danlos syndrome (hEDS), lifetime psychiatric diagnoses, psychiatric rating scales for ASD and attention deficit hyperactivity disorder (ADHD), and socio-demographics was collected for 199 individuals with ASD and 419 non-ASD community controls. Logistic regression models adjusting for covariates (age, sex, ethnicity) revealed a significant relationship between ASD and GJH and between ASD and symptomatic GJH, with adjusted odds ratios of 3.1 (95% CI: 1.9, 5.2;p< 0.001) and 4.9 (95% CI: 2.6, 9.0;p< 0.001), respectively. However, the high prevalence of comorbid ADHD in the study sample reduces the generalizability of the results among individuals with ASD without comorbid ADHD. Possibly, an additional ADHD phenotype is the primary driver of the association between ASD and GJH. Furthermore, GJH with additional self-reported symptoms, suggestive of HSD/hEDS, showed a stronger association with ASD than did non-specified GJH, indicating that symptomatic GJH plays a greater role in the relationship than non-specified GJH does. Therefore, the current study underscores the need of careful sample subclassifications. ASD with GJH may represent a novel subgroup of ASD in terms of aetiopathology and clinical presentation. Future research should elucidate the aetiological factors behind the association between ASD and GJH and evaluate how the comorbidity of GJH affects ASD outcomes.
Improving Healthcare Workers' Adherence to Surgical Safety Checklist: The Impact of a Short Training
Authors: Ferorelli Davide , Benevento Marcello , Vimercati Luigi , Spagnolo Lorenzo , De Maria Luigi , Caputi Antonio , Zotti Fiorenza , Mandarelli Gabriele , Dell'Erba Alessandro , Solarino Biagio
Published: February 2022

Background

Although surgery is essential in healthcare, a significant number of patients suffer unfair harm while undergoing surgery. Many of these originate from failures in non-technical aspects, especially communication among operators. A surgical safety checklist is a simple tool that helps to reduce surgical adverse events, but even if it is fast to fill out, its compilation is often neglected by the healthcare workers because of unprepared cultural background. The present study aims to value the efficacy of a free intervention, such as a short training about risk management and safety checklist, to improve checklist adherence.

Methods

In March 2019, the medical and nursing staff of the General Surgical Unit attended a two-lesson theoretical training concerning surgical safety and risk management tools such as the surgical safety checklist. The authors compared the completeness of the surgical checklists after and before the training, considering the same period (2 months) for both groups.

Result

The surgical safety checklists were present in 198 cases (70.97%) before the intervention and 231 cases (96.25%) after that. After the training, the compilation adherence increased for every different type of healthcare worker of the unit (surgeons, nurses, anesthetists, and scrab nurses). Furthermore, a longer hospitalization was associated with a higher surgical checklist adherence by the operators.

Conclusions

The results showed that a free and simple intervention, such as a two-lesson training, significantly stimulated the correct use of the surgical safety checklist. Moreover, the checklist adherence increased even for the operators who did not attend the training, maybe because of the positive influence of the colleagues' positive behaviors. As the results were promising with only two theoretical lessons, much more can be done to build a new safety culture in healthcare.
Development and Application of a Triplex TaqMan Quantitative Real-Time PCR Assay for Simultaneous Detection of Feline Calicivirus, Feline Parvovirus, and Feline Herpesvirus 1
Authors: Cao Nan , Tang Zhihui , Zhang Xiyu , Li Wanyan , Li Bingxin , Tian Yunbo , Xu Danning
Published: February 2022

As companion animals, felines play an important role in human's family life, and their healthcare has attracted great attention. Viruses such as feline calicivirus (FCV), feline herpesvirus 1 (FHV-1), and feline parvovirus virus (FPV) are the most common pathogens that cause severe infectious disease in baby cats. Thus, preclinical detection and intervention of these three viruses is an effective means to prevent diseases and minimize their danger condition. In this study, a triplex TaqMan quantitative real-time polymerase chain reaction (qRT-PCR) assay was developed to detect these three viruses simultaneously. The detection limit of FPV, FCV, and FHV-1 was 5 × 101copies/assay, which exhibited higher sensitivity (about 10- to100-fold) than conventional PCR. The coefficients of variation (CVs) of the intra-assay variability were lower than 1.86%, and that of inter-assay variability were lower than 3.19%, indicating the excellent repeatability and reproducibility of the triplex assay. Additionally, the assay showed good specificity. Finally, samples from 48 cats were analyzed using the established assay and commercial kits. As a result, the total positive rates for these viruses were 70.83 or 62.5%, respectively, which demonstrated that the developed qRT-PCR assay was more accurate than the commercial kits and could be used in clinical diagnosis.
Producer Perceptions Toward Prevention and Control of Lameness in Dairy Cows in Alberta Canada: A Thematic Analysis
Authors: Knauss Marlena , Adams Cindy L. , Orsel Karin
Published: February 2022

Lameness in dairy cattle poses both an animal welfare and economic threat to dairy farms. Although the Canadian dairy industry has identified lameness as the most important health issue, lameness prevalence in the province of Alberta has not decreased over the last decade. Factors related to lameness have been reported, but the prevalence remains high. Therefore, this study was conducted to investigate dairy producers' perceptions on lameness and how these perceptions influence lameness prevalence in their cows. Qualitative interviews with open-ended questions were conducted with nine dairy producers in Alberta, Canada presenting farms with a wide variety of lameness prevalence. Thematic analysis of these interviews revealed five major themes, as well as five distinct types of producers regarding their perceptions. All nine producers mentioned similar challenges with lameness prevention and control. Identifying lameness, taking action, delays in achieving success, various approaches to prevention and control strategies, and differences between farms were the challenges encountered. However, producers' attitudes when dealing with these challenges varied. We concluded that understanding producers' perceptions is essential as no “one size fits all”, when advising them regarding how to address lameness, as guidance and support will be most successful when it is aligned with their viewpoint.
Integrative Analysis of the lncRNA-Associated ceRNA Regulatory Network Response to Hypoxia in Alveolar Type II Epithelial Cells of Tibetan Pigs
Authors: Yang Yanan , Li Yongqing , Yuan Haonan , Liu Xuanbo , Ren Yue , Gao Caixia , Jiao Ting , Cai Yuan , Zhao Shengguo
Published: February 2022

The function of alveolar type II epithelial (ATII) cells is severely hampered by oxygen deficiency, and understanding the regulatory mechanisms controlling responses to hypoxia may assist in relieving injury induced by hypoxia. In this study, we cultured ATII cells from Tibetan pigs and Landrace pigs under hypoxic and normoxic environments to screen for differentially expressed (DE) lncRNAs, DEmiRNAs, and construct their associated ceRNA regulatory networks in response to hypoxia. Enrichment analysis revealed that target genes of DElncRNAs of Tibetan pigs and Landrace pig between the normoxic (TN, LN) and hypoxic (TL, LL) groups significantly enriched in the proteoglycans in cancer, renal cell carcinoma, and erbB signaling pathways, while the target genes of DEmiRNAs were significantly enriched in the axon guidance, focal adhesion, and mitogen-activated protein kinase (MAPK) signaling pathways. Hypoxia induction was shown to potentially promote apoptosis by activating the focal adhesion/PI3K-Akt/glycolysis pathway. The ssc-miR-20b/MSTRG.57127.1/ssc-miR-7-5p axis potentially played a vital role in alleviating hypoxic injury by regulating ATII cell autophagy under normoxic and hypoxic conditions. MSTRG.14861.4-miR-11971-z-CCDC12, the most affected axis, regulated numerous RNAs and may thus regulate ATII cell growth in Tibetan pigs under hypoxic conditions. The ACTA1/ssc-miR-30c-3p/MSTRG.23871.1 axis is key for limiting ATII cell injury and improving dysfunction and fibrosis mediated by oxidative stress in Landrace pigs. Our findings provide a deeper understanding of the lncRNA/miRNA/mRNA regulatory mechanisms of Tibetan pigs under hypoxic conditions.
Effect of a Probiotic Mixture in Captive Cheetahs (Acinonyx Jubatus) with Gastrointestinal Symptoms—A Pilot Study
Authors: Mangiaterra Sara , Schmidt-Küntzel Anne , Marker Laurie , Di Cerbo Alessandro , Piccinini Renato , Guadagnini Davide , Turba Maria Elena , Berardi Sara , Galosi Livio , Preziuso Silvia , Cerquetella Matteo , Rossi Giacomo
Published: February 2022

Cheetahs (Acinonyx jubatus) are classified as “vulnerable” species due to the low numbers persisting in the wild. Gastrointestinal diseases are very common in this species when they are kept in captivity, in particular gastritis. Clinical signs are predominantly characterized by vomiting, diarrhea, weight loss and anorexia. In this study, we evaluated the efficacy of a multi-strain probiotic in two groups of cheetahs: Group A (n = 4)—rescued cheetahs housed at the Cheetah Conservation Fund (Otjiwarongo, Namibia); Group B (n = 9)—captive cheetahs housed in Italian zoos. Animals showed gastrointestinal signs of different severity, and were positive for Helicobacter spp., detected by PCR in stool samples. Two sachets of probiotic formulation were administered to all cheetahs once a day for 21 consecutive days. Clinical conditions (appetite loss, vomiting, stool consistency and Body Condition Score) before (T0) and after 21 days of probiotic administration (T1) were then compared using a simplified Feline Chronic Enteropathy Activity Index (FCEAI) score. A slight but not significant improvement in the scores was observed in Group A, which had mild intestinal symptoms, while a significant decrease in vomiting and stool consistency (**p < 0.01) scores was observed in Group B, which had more pronounced symptoms. Results suggest that high concentrations of live probiotics can be of help in managing gastrointestinal signs in cheetahs.
Effects of COVID-19-Epidemic-Related Changes in Human Behaviors on Air Quality and Human Health in Metropolitan Parks
Authors: Sung Wen-Pei , Liu Chun-Hao
Published: February 2022

The outbreak of the new coronavirus pneumonia (Coronavirus disease 2019, COVID-19) created a serious impact on the lives of people around the world. Humans, affected by the COVID-19 virus, must reduce related activities to suppress the spread of this disease. However, the pandemic had a positive impact on the environment due to reduced outdoor activities. The correlation between reduced human outdoor activities and health effects was investigated in this study through two Metropolitan parks in Taichung, Taiwan. The developed low-cost air quality sensors were installed in these two parks to detect the variances in PM2.5 concentrations during the epidemic outbreak. Experimental results indicated that PM2.5 concentrations in these two parks were reduced from about 23.25 and 22.96 μg/m3 to 8.19 and 8.48 μg/m3, respectively, the median absolute deviations (MAD) decreased from 4.21 and 4.57 to 1.71 and 1.35, respectively after the epidemic outbreak, and the calculated standard deviation of all normal-to-normal interval (SDNN) and the ratio of low-frequency power to high-frequency (LF/HF) indicated that the drops of PM2.5 concentrations caused the increased health-related benefits by 73.53% with the variances being low. These results showed that the PM2.5 concentrations displayed high correlations with human activities, which also played important roles in human health effects.
Assessment of Modulus Attenuation of Cement and Lime-Fly Ash Semi-Rigid Road Base Materials
Authors: Chen Luchuan , Yu Sixin , Zhu Ying , Zhang Xiaomeng , Wu Wenjuan , Sun Qiang , Chen Tingting , Wang Xiaoyan , Wei Jincheng
Published: February 2022

For asphalt pavement structures, semi-rigid road base course has to sustain repeated high-axle load during its service life and the performance of semi-rigid road base materials directly influences the durability of pavement structures. The dynamic compressive resilience modulus of two commonly used semi-rigid road base materials, cement stabilized aggregates (CSG) and lime-fly ash stabilized aggregates (LFSG) were evaluated at different frequencies using a Universal Testing Machine (UTM). The results showed that LFSG had higher dynamic modulus than that of CSG and the load frequency had less influence on the dynamic modulus of these two semi-rigid road base materials. The four-point bending test was applied to measure the flexural-bending strength and the fatigue life of these two semi-rigid materials. A higher flexural-bending strength of LFSG indicated its better bearing capacity than that of CSG. The fatigue life of LFSG and CSG decreased with the increase of stress–strength ratio and the LFSG performed better in terms of fatigue resistance. The fatigue damage models of CSG and LFSG based on Stress-Life (S-N) curve are established. As per incremental-recursive mechanics, a general modulus degradation model was established and verified by the results of full-scale accelerate loading test. This model cannot only be used to predict the fatigue deterioration of semi-rigid road base materials under different stress levels, but is also able to calculate the current bending elastic modulus based on its initial modulus value.
Spatiotemporal Dynamics and Factors Driving the Distributions of Pine Wilt Disease-Damaged Forests in China
Authors: Wang Wei , Peng Wanting , Liu Xiuyu , He Geng , Cai Yongli
Published: February 2022

Many forests have suffered serious economic losses and ecological consequences of pine wilt disease (PWD) outbreaks. Climate change and human activities could accelerate the distribution of PWD, causing the exponential expansion of damaged forest areas in China. However, few studies have analyzed the spatiotemporal dynamics and the factors driving the distribution of PWD-damaged forests using continuous records of long-term damage, focusing on short-term environmental factors that influence multiple PWD outbreaks. We used a maximum entropy (MaxEnt) model that incorporated annual meteorological and human activity factors, as well as temporal dependence (the PWD distribution in the previous year), to determine the contributions of environmental factors to the annual distribution of PWD-damaged forests in the period 1982–2020. Overall, the MaxEnt showed good performance in modeling the PWD-damaged forest distributions between 1982 and 2020. Our results indicate that (i) the temporal lag dependence term for the presence/absence of PWD was the best predictor of the distribution of PWD-damaged forests; and (ii) Bio14 (precipitation in the driest month) was the most important meteorological factor for affecting the PWD-damaged forests. These results are essential to understanding the factors governing the distribution of PWD-damaged forests, which is important for forest management and pest control worldwide.