bluuebunny/crossref_metadata_2025 huggingface dataset:
Role of Artificial Intelligence in Shaping Consumer Demand in E-Commerce
Authors: Khrais Laith T.
Published: December 2020

The advent and incorporation of technology in businesses have reformed operations across industries. Notably, major technical shifts in e-commerce aim to influence customer behavior in favor of some products and brands. Artificial intelligence (AI) comes on board as an essential innovative tool for personalization and customizing products to meet specific demands. This research finds that, despite the contribution of AI systems in e-commerce, its ethical soundness is a contentious issue, especially regarding the concept of explainability. The study adopted the use of word cloud analysis, voyance analysis, and concordance analysis to gain a detailed understanding of the idea of explainability as has been utilized by researchers in the context of AI. Motivated by a corpus analysis, this research lays the groundwork for a uniform front, thus contributing to a scientific breakthrough that seeks to formulate Explainable Artificial Intelligence (XAI) models. XAI is a machine learning field that inspects and tries to understand the models and steps involved in how the black box decisions of AI systems are made; it provides insights into the decision points, variables, and data used to make a recommendation. This study suggested that, to deploy explainable XAI systems, ML models should be improved, making them interpretable and comprehensible.
Vertebrae Morphometric Measurement and Ca/P Levels of Different Age European Seabass (Dicentrarchus labrax)
Authors: Nikiforidou Vasiliki , Zaoutsos Stefanos , Vlahos Nikolaos , Berillis Panagiotis
Published: December 2020

The European seabass is one of the most important species of the Mediterranean, specifically Greece. Individuals with different numbers of vertebrae have been reported. This number ranges from 24 to 26 vertebrae. In this study a sample of 73 individual seabass were collected from fish farms and divided into three age groups. The first group included fingerling individuals, the second group, juvenile individuals and the third group, adult individuals. The number and the length of their vertebrae were measured by radiographs. The individuals were divided into subgroups according to their vertebrae number, and from each one the tenth vertebra was taken. Ca and P levels (%) of each tenth vertebra were measured by X-ray spectroscopy (EDS), and the Ca/P ratio was determined. Vertebrae length, Ca and P levels and Ca/P ratio were compared among age groups and among individuals with different numbers of vertebrae. It was shown that the European seabass’s vertebral column can be divided to three sections—cervical, abdominal and caudal—following the striped bass (Morone saxatilis) model.
Deep Ocean Water Concentrate Changes Physicochemical Characteristics, the Profile of Volatile Components and Consumer Acceptance for Taiwanese Rice Shochu
Authors: Shih Ming-Kuei , Hsu Qiao-Yu , Liou Bo-Kang , Peng Yu-Han , Hou Chih-Yao
Published: December 2020

To study the effects of deep-ocean water concentrate (DOWC) on sake quality, Taichung No. 10 indica rice (Oryza sativa subsp. indica) and Tainan No. 11 japonica rice (O. sativa subsp. japonica) were used as raw materials, and basic physicochemical property parameters in shochu were analyzed differentially. Sake fermentation mash analysis results revealed that DOWC addition did not significantly affect the basic physicochemical properties during sake brewing, but it significantly reduced citric acid and malic acid contents in Taichung No. 10 indica rice sake sample by 52–66% and 73–93%, respectively. DOWC addition significantly increased citric acid content in Tainan No. 11 japonica rice sake sample by 32–202%. Rice shochu analysis results revealed that DOWC addition significantly increased isoamyl acetate, ethyl hexanoate, and ethyl octanoate contents in shochu made from japonica rice and indica rice, respectively. The results indicate that rice variety directly affects the types of volatile compounds in rice shochu. Principal component analysis and sensory evaluation results revealed that DOWC addition affected the composition of volatile compounds in the two types of rice shochu and resulted in differences in flavor evaluation. DOWC addition affects yeast metabolites and directly changes the volatile compound composition and flavor of rice shochu.
Network and Evolutionary Analysis of Human Epigenetic Regulators to Unravel Disease Associations
Authors: Ohsawa Shinji , Umemura Toshiaki , Terada Tomoyoshi , Muto Yoshinori
Published: December 2020

We carried out a system-level analysis of epigenetic regulators (ERs) and detailed the protein–protein interaction (PPI) network characteristics of disease-associated ERs. We found that most diseases associated with ERs can be clustered into two large groups, cancer diseases and developmental diseases. ER genes formed a highly interconnected PPI subnetwork, indicating a high tendency to interact and agglomerate with one another. We used the disease module detection (DIAMOnD) algorithm to expand the PPI subnetworks into a comprehensive cancer disease ER network (CDEN) and developmental disease ER network (DDEN). Using the transcriptome from early mouse developmental stages, we identified the gene co-expression modules significantly enriched for the CDEN and DDEN gene sets, which indicated the stage-dependent roles of ER-related disease genes during early embryonic development. The evolutionary rate and phylogenetic age distribution analysis indicated that the evolution of CDEN and DDEN genes was mostly constrained, and these genes exhibited older evolutionary age. Our analysis of human polymorphism data revealed that genes belonging to DDEN and Seed-DDEN were more likely to show signs of recent positive selection in human history. This finding suggests a potential association between positive selection of ERs and risk of developmental diseases through the mechanism of antagonistic pleiotropy.
The Association of Multiple Gene Variants with Ageing Skeletal Muscle Phenotypes in Elderly Women
Authors: Khanal Praval , He Lingxiao , Herbert Adam J. , Stebbings Georgina K. , Onambele-Pearson Gladys L. , Degens Hans , Morse Christopher I. , Thomis Martine , Williams Alun G.
Published: December 2020

There is a scarcity of studies that have investigated the role of multiple single nucleotide polymorphisms (SNPs) on a range of muscle phenotypes in an elderly population. The present study investigated the possible association of 24 SNPs with skeletal muscle phenotypes in 307 elderly Caucasian women (aged 60–91 years, 66.3 ± 11.3 kg). Skeletal muscle phenotypes included biceps brachii thickness, vastus lateralis cross-sectional areas, maximal hand grip strength, isometric knee extension and elbow flexion torque. Genotyping for 24 SNPs, chosen on their skeletal muscle structural or functional links, was conducted on DNA extracted from blood or saliva. Of the 24 SNPs, 10 were associated with at least one skeletal muscle phenotype. HIF1A rs11549465 was associated with three skeletal muscle phenotypes and PTK2 rs7460 and ACVR1B rs10783485 were each associated with two phenotypes. PTK2 rs7843014, COL1A1 rs1800012, CNTF rs1800169, NOS3 rs1799983, MSTN rs1805086, TRHR rs7832552 and FTO rs9939609 were each associated with one. Elderly women possessing favourable genotypes were 3.6–13.2% stronger and had 4.6–14.7% larger muscle than those with less favourable genotypes. These associations, together with future work involving a broader range of SNPs, may help identify individuals at particular risk of an age-associated loss of independence.
Present and Future Salmonid Cytogenetics
Authors: Gaffaroglu Muhammet , Majtánová Zuzana , Symonová Radka , Pelikánová Šárka , Unal Sevgi , Lajbner Zdeněk , Ráb Petr
Published: December 2020

Salmonids are extremely important economically and scientifically; therefore, dynamic developments in their research have occurred and will continue occurring in the future. At the same time, their complex phylogeny and taxonomy are challenging for traditional approaches in research. Here, we first provide discoveries regarding the hitherto completely unknown cytogenetic characteristics of the Anatolian endemic flathead trout, Salmo platycephalus, and summarize the presently known, albeit highly complicated, situation in the genus Salmo. Secondly, by outlining future directions of salmonid cytogenomics, we have produced a prototypical virtual karyotype of Salmo trutta, the closest relative of S. platycephalus. This production is now possible thanks to the high-quality genome assembled to the chromosome level in S. trutta via soft-masking, including a direct labelling of repetitive sequences along the chromosome sequence. Repetitive sequences were crucial for traditional fish cytogenetics and hence should also be utilized in fish cytogenomics. As such virtual karyotypes become increasingly available in the very near future, it is necessary to integrate both present and future approaches to maximize their respective benefits. Finally, we show how the presumably repetitive sequences in salmonids can change the understanding of the overall relationship between genome size and G+C content, creating another outstanding question in salmonid cytogenomics waiting to be resolved.
Unusual Longevity of Edwards Syndrome: A Case Report
Authors: Alshami Abbas , Douedi Steven , Guida Melissa , Ajam Firas , Desai Dhaval , Zales Vincent , Calderon Dawn M
Published: December 2020

Background: Trisomy 18, also known as Edwards syndrome, was first described in the 1960s and is now defined as the second most common trisomy. While this genetic disease has been attributed to nondisjunction during meiosis, the exact mechanism remains unknown. Trisomy 18 is associated with a significantly increased mortality rate of about 5–10% of patients surviving until 1 year of age. We present a case of a 26-year-old female diagnosed with trisomy 18, well outliving her life expectancy, maintaining a stable state of health. Case Presentation: A 26-year-old female with non-mosaic Edwards syndrome presented to the clinic for follow up after recent hospitalization for aspiration pneumonia. The definitive diagnosis of trisomy 18 was made prenatally utilizing chromosomal analysis and G-banding and fluorescence in situ hybridization (FISH) on cells obtained via amniocentesis. Her past medical history is characterized by severe growth and intellectual limitations; recurrent history of infections, especially respiratory system infections; and a ventricular septal defect (VSD) that was never surgically repaired. She remains in good, stable health and is under close follow-up and monitoring. Conclusions: Despite the fact that Edwards syndrome carries a significantly high mortality rate due to several comorbidities, recent literature including this case report has identified patients surviving into adulthood. Advancements in early detection and parent education have likely allowed for these findings. We aim to present a case of an adult with trisomy 18, living in stable condition, with an importance on medical follow-up.
Children’s Independent Mobility to School in Seven European Countries: A Multinomial Logit Model
Authors: Masoumi Houshmand , Rooijen Martin van , Sierpiński Grzegorz
Published: December 2020

The determinants of children’s independent school mobility and the contextual discrepancies between these determinants have not been comprehensively investigated in previous studies. It is important to examine these determinants because independent school mobility is associated with children’s physical activity, according to the literature. This paper examined the associations of different groups of variables such as household, mobility, perceptions, and the built environment with independent school mobility of children between 9 and 12 years using a sample of 1304 girls (50.9%) and boys (49.1%) in seven European countries. The sample was analyzed by Multinomial Logistic Regression, Chi-square test of independence, and Proportional Reduction in Error methods. According to the findings, father’s and mother’s commute mode choice, child’s mode choice of commute to school, child’s bike ownership, parent’s perception of safety, parent’s evaluation of bike lane and sidewalk quality, child’s commute distance, number of driving licenses in the household, accessibility of public transport, and population density in the neighborhood and around the school proved to be very strong and significant determinants of children’s independent school mobility in the Europe-wide sample. The comparison of the levels of independent school mobility did not show any significant differences between high-income countries such as Germany, Italy, and the Netherlands, and emerging economies and developing countries like Poland, Greece, Turkey, and Croatia. However, a direct comparison between Poland (emerging economy) (33.6%) and the Netherlands (high-income) (31.7%) revealed significant differences in the level of independent school mobility. This study found the motives for this discrepancy due to the significant difference in bike ownership, the number of household members working outside of the house, household size, commute distances of parents, and driving license possession.
Realtors’ Perceptions of Social and Physical Neighborhood Characteristics Associated with Active Living: A Canadian Perspective
Authors: McCormack Gavin R. , Nesdoly Autumn , Ghoneim Dalia , McHugh Tara-Leigh
Published: December 2020

Realtors match home-seekers with neighborhoods that have built and social characteristics they desire to pursue active lifestyles. Studies have yet to explore realtors’ perspectives on neighborhood design that supports active living. Using qualitative description, our study was to explore the perceptions and understandings of neighborhood design (walkability, healthy, bike-ability, vibrancy, and livability) among urban residential realtors. Nineteen (6 men; 13 women; average age 48 years) self-identified residential realtors from Calgary, Edmonton, and Lethbridge (Canada) completed semi-structured telephone interviews. Content analysis identified themes from the interview data. Specifically, walkability was described as: perceived preferences, destinations and amenities, and connections; a healthy community was described as: encourages outdoor activities, and promotes social homogeneity; bike-ability was described as: bike-ability attributes, and was controversial; vibrancy was described as: community feel, and evidence of life; and livability was described as: subjective, and preferences and necessities. Our findings can inform the refinement of universal definitions and concepts used to in neighborhood urban design.
The Response of Arbuscular Mycorrhizal Fungal Communities to the Soil Environment of Underground Mining Subsidence Area in Northwest China
Authors: Huang Hai , Guo Jing , Zhang Yuxiu
Published: December 2020

Fully mechanized mining technology applied over a very large working face is typically utilized for coal exploitation in Northwest China and triggered two types of land subsidence above the goaf edge and center. However, the effects of mining subsidence on arbuscular mycorrhizal fungal (AMF) communities are still unknown. Here, we investigated the soil physicochemical properties and the response of AMF communities to the soil environment at the margin and center of the subsidence area of the same working face. Our results showed the soil water content, nutrient content and enzyme activity were significantly decreased with land desertification at the margin of the subsidence area but were less affected at the subsidence center. Utilizing high-throughput sequence analysis, six Glomeromycotan genera were detected. The relative abundance of Glomus and Ambispora at the margin of the subsidence area decreased, while Paraglomus and Diversispora increased. The total OTU richness was significantly correlated with moisture. Redundancy analysis showed the main environmental factors driving the changes in AMF community structure were available nitrogen, available potassium and available phosphorus. All these results indicated land cracks need to be repaired in time at subsidence edge to prevent the decline of soil fertility.
Concerted Cultivation and Adolescent Psychopathology over Time-Mediation of Parent-Child Conflict
Authors: Leung Janet T. Y.
Published: December 2020

Background: Concerted cultivation is a parenting strategy that parents nurture their children intensively by involving heavily in their children’s academic sphere as well as offering them different structured “enrichment” activities so that their children can succeed in the future competitive “rug rat race”. While this parenting strategy has been regarded as an effective strategy to promote child and adolescent development, it is deemed to create stress and anxiety for their children. The present study examined the relationship between concerted cultivation and adolescent psychopathology (indexed by depression and anxiety) via parent–child conflict among Chinese adolescents in Hong Kong over time. Method: A sample of 1570 young adolescents (48.5% girls, mean age at time 1 = 12.6, SD = 0.76) were recruited from 19 secondary schools in Hong Kong. Adolescents were invited to fill out a questionnaire that contained measures of concerted cultivation, parent–child conflict, anxiety and depression in two consecutive years. Results: Results from structural equation modeling showed that higher levels of paternal concerted cultivation were associated with higher levels of adolescent psychopathology via increased father–child conflict over time. However, maternal concerted cultivation was linked to greater mother–child conflict but reduced father-child conflict, which was associated with adolescent psychopathology. Discussion: Rather than regarding concerted cultivation as an effective parenting strategy that promotes adolescent development, the findings indicated that concerted cultivation increased adolescent psychopathology via increased parent–child conflict. The study sheds new light for family practitioners and educators in their awareness of the adverse effects of concerted cultivation and designing appropriate parent education programs for parents.
Is the C-C Motif Ligand 2–C-C Chemokine Receptor 2 Axis a Promising Target for Cancer Therapy and Diagnosis?
Authors: Iwamoto Hiroaki , Izumi Kouji , Mizokami Atsushi
Published: December 2020

C-C motif ligand 2 (CCL2) was originally reported as a chemical mediator attracting mononuclear cells to inflammatory tissue. Many studies have reported that CCL2 can directly activate cancer cells through a variety of mechanisms. CCL2 can also promote cancer progression indirectly through increasing the recruitment of tumor-associated macrophages into the tumor microenvironment. The role of CCL2 in cancer progression has gradually been understood, and various preclinical cancer models elucidate that CCL2 and its receptor C-C chemokine receptor 2 (CCR2) are attractive targets for intervention in cancer development. However, clinically available drugs that regulate the CCL2–CCR2 axis as anticancer agents are not available at this time. The complete elucidation of not only the oncological but also the physiological functions of the CCL2–CCR2 axis is required for achieving a satisfactory effect of the CCL2–CCR2 axis-targeted therapy.
Genomic Instability Is an Early Event in Aluminium-Induced Tumorigenesis
Authors: Mandriota Stefano J. , Tenan Mirna , Nicolle Adeline , Jankowska Julia D. , Ferrari Paolo , Tille Jean-Christophe , Durin Mary-Anne , Green Catherine M. , Tabruyn Sebastien , Moralli Daniela , Sappino André-Pascal
Published: December 2020

Genomic instability is generally considered as a hallmark of tumorigenesis and a prerequisite condition for malignant transformation. Aluminium salts are suspected environmental carcinogens that transform mammary epithelial cells in vitro through unknown mechanisms. We report here that long-term culture in the presence of aluminium chloride (AlCl3) enables HC11 normal mouse mammary epithelial cells to form tumours and metastases when injected into the syngeneic and immunocompetent BALB/cByJ strain. We demonstrate that AlCl3 rapidly increases chromosomal structural abnormalities in mammary epithelial cells, while we failed to detect direct modulation of specific mRNA pathways. Our observations provide evidence that clastogenic activity—a well-recognized inducer of genomic instability—might account in part for the transforming abilities of aluminium in mammary epithelial cells.
Angiotensin-(1-7) Prevents Lipopolysaccharide-Induced Autophagy via the Mas Receptor in Skeletal Muscle
Authors: Rivera Juan Carlos , Abrigo Johanna , Tacchi Franco , Simon Felipe , Brandan Enrique , Santos Robson A. , Bader Michael , Chiong Mario , Cabello-Verrugio Claudio
Published: December 2020

Skeletal muscle atrophy, which occurs in lipopolysaccharide (LPS)-induced sepsis, causes a severe muscle function reduction. The increased autophagy contributes to sepsis-induced skeletal muscle atrophy in a model of LPS injection, increasing LC3II/LC3I ratio, autophagy flux, and autophagosomes. Angiotensin-(1-7) (Ang-(1-7)) has anti-atrophic effects via the Mas receptor in skeletal muscle. However, the impact of Ang-(1-7) on LPS-induced autophagy is unknown. In this study, we determined the effect of Ang-(1-7) on sepsis-induced muscle autophagy. C57BL6 wild-type (WT) mice and mice lacking the Mas receptor (KO Mas) were injected with LPS together with the systemic administration of Ang-(1-7) to determine autophagy in skeletal muscle. We also evaluated autophagy and p38 and c-Jun N-terminal kinase (JNK)activation. Our results show that Ang-(1-7) prevents LPS-induced autophagy in the diaphragm, tibialis anterior, and gastrocnemius of WT mice, which is demonstrated by a decrease in the LC3II/LC3I ratio and mRNA levels of lc3b and ctsl. This effect was lost in KO Mas mice, suggesting the role of the Mas receptor. The results in C2C12 cells show that Ang-(1-7) reduces several LPS-dependent effects, such as autophagy (LC3II/LC3I ratio, autophagic flux, and autophagosomes), activation of p38 and JNK, B-cell lymphoma-2 (BCL2) phosphorylation, and disassembly of the Beclin1/BCL2 complex. In conclusion, Ang-(1-7)/Mas receptor reduces LPS-induced autophagy in skeletal muscle. In vitro assays indicate that Ang-(1-7) prevents LPS-induced autophagy and modifies the MAPK signaling and the disassembly of a complex involved at the beginning of autophagy.
Anti-Stem Cell Property of Pterostilbene in Gastrointestinal Cancer Cells
Authors: Mori Shiori , Kishi Shingo , Honoki Kanya , Fujiwara-Tani Rina , Moriguchi Takuma , Sasaki Takamitsu , Fujii Kiyomu , Tsukamoto Shinji , Fujii Hiromasa , Kido Akira , Tanaka Yasuhito , Luo Yi , Kuniyasu Hiroki
Published: December 2020

Pterostilbene (PTE) is a natural sterbenoid contained in blueberries that has an antioxidant effect. In contrast, PTE also generates oxidative stress in cancer cells and provides an antitumor effect. Here, we examined the potential mechanism of this contrasting effect of PTE using three gastrointestinal cancer cell lines, namely CT26, HT29, and MKN74. PTE showed a dose-dependent inhibition of cell proliferation, sphere-forming ability, and stem cell marker expression in all three cell lines. Furthermore, the cells treated with PTE showed an increase in mitochondrial membrane potential and an increase in mitochondrial oxidative stress and lipid peroxide. Upon concurrent treatment with vitamin E, N-acetyl-L-cysteine, and PTE, the PTE-induced mitochondrial oxidative stress and growth inhibition were suppressed. These findings indicate that PTE induces oxidative stress in cancer cells, suppresses stemness, and inhibits proliferation. These antitumor effects of PTE are considered to be useful in cancer treatment.
Disruption of Cell Adhesion and Cytoskeletal Networks by Thiol-Functionalized Silica-Coated Iron Oxide Nanoparticles
Authors: Královec Karel , Melounková Lucie , Slováková Marcela , Mannová Nikola , Sedlák Miloš , Bartáček Jan , Havelek Radim
Published: December 2020

One of the major obstacles that limits the use of magnetic nanoparticles in biomedical applications is their potential toxicity. In the present study, we evaluated the cytotoxic effects of thiol-functionalized silica-coated iron oxide (Fe3O4@SiO2-SH) nanoparticles using human lung epithelial cells A549. We investigated the effect of Fe3O4@SiO2-SH nanoparticles on the cell viability, proliferation, cell cycle distribution, adhesion, apoptosis, and the orientation of the cytoskeletal networks, as well as on expression of proteins involved in cell death, cell survival, and cell adhesion. We demonstrated that exposure of A549 cells to Fe3O4@SiO2-SH nanoparticles resulted in severe disruption of the actin microfilaments and microtubule cytoskeleton and reduced the size of focal adhesions. Furthermore, cell adhesion was significantly affected as well as the phosphorylation of focal adhesion kinase (FAK), extracellular-signal-regulated kinase (ERK), and p38. Our findings highlight the need for in-depth cytotoxic evaluation of nanoparticles supporting their safer use, especially in biomedical applications.
The Inhibitory Role of Rab11b in Osteoclastogenesis through Triggering Lysosome-Induced Degradation of c-Fms and RANK Surface Receptors
Authors: Tran Manh Tien , Okusha Yuka , Feng Yunxia , Morimatsu Masatoshi , Wei Penggong , Sogawa Chiharu , Eguchi Takanori , Kadowaki Tomoko , Sakai Eiko , Okamura Hirohiko , Naruse Keiji , Tsukuba Takayuki , Okamoto Kuniaki
Published: December 2020

Rab11b, abundantly enriched in endocytic recycling compartments, is required for the establishment of the machinery of vesicle trafficking. Yet, no report has so far characterized the biological function of Rab11b in osteoclastogenesis. Using in vitro model of osteoclasts differentiated from murine macrophages like RAW-D cells or bone marrow-derived macrophages, we elucidated that Rab11b served as an inhibitory regulator of osteoclast differentiation sequentially via (i) abolishing surface abundance of RANK and c-Fms receptors; and (ii) attenuating nuclear factor of activated T-cells c1 (NFATc-1) upstream signaling cascades, following RANKL stimulation. Rab11b was localized in early and late endosomes, Golgi complex, and endoplasmic reticulum; moreover, its overexpression enlarged early and late endosomes. Upon inhibition of lysosomal function by a specific blocker, chloroquine (CLQ), we comprehensively clarified a novel function of lysosomes on mediating proteolytic degradation of c-Fms and RANK surface receptors, drastically ameliorated by Rab11b overexpression in RAW-D cell-derived osteoclasts. These findings highlight the key role of Rab11b as an inhibitor of osteoclastogenesis by directing the transport of c-Fms and RANK surface receptors to lysosomes for degradation via the axis of early endosomes-late endosomes-lysosomes, thereby contributing towards the systemic equilibrium of the bone resorption phase.
Establishment of a p53 Null Murine Oral Carcinoma Cell Line and the Identification of Genetic Alterations Associated with This Carcinoma
Authors: Chang Kuo-Wei , Lin Chia-En , Tu Hsi-Feng , Chung Hsin-Yao , Chen Yi-Fen , Lin Shu-Chun
Published: December 2020

Head and neck squamous cell carcinoma (HNSCC), including oral squamous cell carcinoma (OSCC), ranks sixth in cancer incidence worldwide. To generate OSCC cells lines from human or murine tumors, greatly facilitates investigations into OSCC. This study describes the establishing of a mouse palatal carcinoma cell line (designated MPC-1) from a spontaneous tumor present in a heterozygous p53 gene loss C57BL/6 mouse. A MPC-1-GFP cell subclone was then generated by lentivirus infection resulting in stable expression of green fluorescent protein. Assays indicated that MPC-1 was a p53 null polygonal cell that was positive for keratinocyte markers; it also expressed vimentin and showed a loss of E-cadherin expression. Despite that MPC-1 having strong proliferation and colony formation capabilities, the potential for anchorage independent growth and tumorigenesis was almost absent. Like other murine MOC-L and MTCQ cell line series we have previously established, MPC-1 also expresses a range of stemness markers, various oncogenic proteins, and a number of immune checkpoint proteins at high levels. However, the synergistic effects of the CDK4/6 inhibitor palbociclib on other therapeutic drugs were not observed with MPC-1. Whole exon sequencing revealed that there were high rates of non-synonymous mutations in MPC-1 affecting various genes, including Akap9, Arap2, Cdh11, Hjurp, Mroh2a, Muc4, Muc6, Sp110, and Sp140, which are similar to that the mutations present in a panel of chemical carcinogenesis-related murine tongue carcinoma cell lines. Analysis has highlighted the dis-regulation of Akap9, Cdh11, Muc4, Sp110, and Sp140 in human HNSCC as indicated by the TCGA and GEO OSCC databases. Sp140 expression has also been associated with patient survival. This study describes the establishment and characterization of the MPC-1 cell line and this new cell model should help to advance genetic research into oral cancer.
Nonlinear Destructive Interaction between Wind and Wave Loads Acting on the Substructure of the Offshore Wind Energy Converter: A Numerical Study
Authors: Cho Yong Jun
Published: December 2020

Even though the offshore wind industry’s growth potential is immense, the offshore wind industry is still suffering from problems, such as the large initial capital requirements. Many factors are involved, and among these, the extra costs incurred by the conservative design of offshore wind energy converters can be quickly addressed at the design stage by accounting for the nonlinear destructive interaction between wind and wave loads. Even when waves approach offshore wind energy converters collinearly with the wind, waves and wind do not always make the offshore wind energy converter’s substructure deformed. These environmental loads can intermittently exert a force of resistance against deformation due to the nonlinear destructive interaction between wind and wave loads. Hence, the nonlinear destructive interaction between wave and wind loads deserves much more attention. Otherwise, a very conservative design of offshore wind energy converters will hamper the offshore wind energy industry’s development, which is already suffering from enormous initial capital expenditures. In this rationale, this study numerically simulates a 5 MW offshore wind energy converter’s structural behavior subject to wind and random waves using the dynamic structural model developed to examine the nonlinear destructive interaction between wind and wave loads. Numerical results show that the randomly fluctuating water surface as the wind blows would restrict the offshore wind energy converter’s substructure’s deflection. Nonuniform growth of the atmospheric boundary layer due to the wavy motions at the water surface as the wind blows results in a series of hairpin vortices, which lead to the development of a large eddy out of hairpin vortices swirling in the direction opposite to the incoming wind near the atmospheric boundary layer. As a result, the vertical profile of the longitudinal wind velocity is modified; the subsequent energy loss drastically weakens the wind velocity, which consequently leads to the smaller deflection of the substructure of the offshore wind energy converter by 50% when compared with that in the case of wind with gusts over a calm sea.
Glucosylceramide Plays a Role in Fungal Germination, Lipid Raft Organization and Biofilm Adhesion of the Pathogenic Fungus Scedosporium aurantiacum
Authors: Rochetti Victor Pereira , Rollin-Pinheiro Rodrigo , de Oliveira Evely Bertulino , Xisto Mariana Ingrid Dutra da Silva , Barreto-Bergter Eliana
Published: December 2020

Infections caused by Scedosporium species present a wide range of clinical manifestations, from superficial to disseminated, especially in immunocompromised patients. Glucosylceramides (GlcCer) are glycosphingolipids found on the fungal cell surface and play an important role in growth and pathogenicity processes in different fungi. The present study aimed to evaluate the structure of GlcCer and its role during growth in two S. aurantiacum isolates. Purified GlcCer from both isolates were obtained and its chemical structure identified by mass spectrometry. Using ELISA and immunofluorescence techniques it was observed that germination and NaOH-treatment of conidia favor GlcCer exposure. Monoclonal anti-GlcCer antibody reduced germination when cultivated with the inhibitor of melanin synthesis tricyclazole and also reduced germ tube length of conidia, both cultivated or not with tricyclazole. It was also demonstrated that anti-GlcCer altered lipid rafts organization, as shown by using the fluorescent stain filipin, but did not affect the susceptibility of the cell surface to damaging agents. Anti-GlcCer reduced total biomass and viability in biofilms formed on polystyrene plates. In the presence of anti-GlcCer, germinated S. aurantiacum conidia and biofilms could not adhere to polystyrene with the same efficacy as control cells. These results highlight the relevance of GlcCer in growth processes of S. aurantiacum.
Human Pathogenic Candida Species Respond Distinctively to Lactic Acid Stress
Authors: Zangl Isabella , Beyer Reinhard , Pap Ildiko-Julia , Strauss Joseph , Aspöck Christoph , Willinger Birgit , Schüller Christoph
Published: December 2020

Several Candida species are opportunistic human fungal pathogens and thrive in various environmental niches in and on the human body. In this study we focus on the conditions of the vaginal tract, which is acidic, hypoxic, glucose-deprived, and contains lactic acid. We quantitatively analyze the lactic acid tolerance in glucose-rich and glucose-deprived environment of five Candida species: Candidaalbicans, Candida glabrata, Candida parapsilosis, Candida krusei and Candida tropicalis. To characterize the phenotypic space, we analyzed 40–100 clinical isolates of each species. Each Candida species had a very distinct response pattern to lactic acid stress and characteristic phenotypic variability. C. glabrata and C. parapsilosis were best to withstand high concentrations of lactic acid with glucose as carbon source. A glucose-deprived environment induced lactic acid stress tolerance in all species. With lactate as carbon source the growth rate of C. krusei is even higher compared to glucose, whereas the other species grow slower. C. krusei may use lactic acid as carbon source in the vaginal tract. Stress resistance variability was highest among C. parapsilosis strains. In conclusion, each Candida spp. is adapted differently to cope with lactic acid stress and resistant to physiological concentrations.
Models for Expected Returns with Statistical Factors
Authors: Cueto José Manuel , Grané Aurea , Cascos Ignacio
Published: December 2020

In this paper, we propose multifactor models for the pan-European Equity Market using a block-bootstrap method and compare the results with those of traditional inferential techniques. The new factors are built from statistical measurements on stock prices—in particular, coefficient of variation, skewness, and kurtosis. Data come from Reuters, correspond to nearly 2000 EU companies, and span from January 2008 to February 2018. Regarding methodology, we propose a non-parametric resampling procedure that accounts for time dependency in order to test the validity of the model and the significance of the parameters involved. We compare our bootstrap-based inferential results with classical proposals (based on F-statistics). Methods under assessment are time-series regression, cross-sectional regression, and the Fama–MacBeth procedure. The main findings indicate that the two factors that better improve the Capital Asset Pricing Model with regard to the adjusted R2 in the time-series regressions are the skewness and the coefficient of variation. For this reason, a model including those two factors together with the market is thoroughly studied. We also observe that our block-bootstrap methodology seems to be more conservative with the null of the GRS test than classical procedures.
Orientation Identification of the Black Phosphorus with Different Thickness Based on B2g Mode Using a Micro-Raman Spectroscope under a Nonanalyzer Configuration
Authors: Li Rubing , Shang Yongchao , Xing Huadan , Wang Xiaojie , Sun Mingyuan , Qiu Wei
Published: December 2020

As an anisotropic material, the unique optoelectronic properties of black phosphorus are obviously anisotropic. Therefore, non-destructive and fast identification of its crystalline orientation is an important condition for its application in optoelectronics research field. Identifying the crystalline orientation of black phosphorus through Ag1 and Ag2 modes under the parallel polarization has high requirements on the Raman system, while in the nonanalyzer configuration, the crystalline orientation of the thick black phosphorus may not be identified through Ag1 and Ag2 modes. This work proposes a new method to identify the crystalline orientation of black phosphorus of different thicknesses. This method is conducted under the nonanalyzer configuration by B2g mode. The results show that B2g mode has a good consistency in the identification of crystalline orientations. In this paper, a theoretical model is established to study the angle-resolved Raman results of B2g mode. The new method can accurately identify the crystalline orientation with different layers of black phosphorus without misidentification.
Using Silane Coupling Agent Coating on Acidic Aggregate Surfaces to Enhance the Adhesion between Asphalt and Aggregate: A Molecular Dynamics Simulation
Authors: Ding Gongying , Yu Xin , Dong Fuqiang , Ji Zezhong , Wang Junyan
Published: December 2020

Acidic aggregates have the merits of high strength and good abrasion resistance capacity. However, its poor adhesion with asphalt binder constrains its application in pavement construction. Among these, the granite aggregate is the typical one. Therefore, this study modified granite aggregates’ surface to improve their adhesion property with the asphalt binder. Specifically, the silane coupling agent (SCA) KH-560 was adopted to achieve the modification purpose. Subsequently, asphalt mixtures with modified and unmodified granite, basalt, and limestone were subjected to the boiling test, immersion test, and freeze-thaw splitting test to estimate the asphalt adhesion property. Moreover, a molecular dynamic simulation was employed to characterize the asphalt-aggregate interface from the molecular scale. The radius distribution function (RDF) and interaction energy were used as the primary indicators. The results showed that the SCA could efficiently improve the adhesion between asphalt and granite aggregates, comparable with the alkaline aggregates. In terms of the molecular scale, the incorporation of SCA could significantly increase the concentration distribution of asphalt molecules on the aggregate surface. Meanwhile, the interaction energy was correspondingly increased due to the considerable growth of non-bond interaction.
Analysis of Deformation and Prediction of Cracks in the Cogging Process for Die Steel at Elevated Temperatures
Authors: Kukuryk Marcin
Published: December 2020

In this paper, an analysis of a three-dimensional state of strain and stress in the case of the hot cogging process of X32CrMoV12-28 die steel with the application of the finite element method is presented. The results of the investigations connected with the simulation of the kinematics of metal flow and thermal phenomena are presented, accompanied by prognosing the formation of ductile fractures in the course of the hot cogging process conducted with the application of three different shape tools and of a proposed deformation criterion of the loss of cohesion. The applied anvils were found to be highly effective in the aspects of distribution of effective strains and stresses, absence of tensile stresses in the axial zones of a forging, and also of a significant thermal stability in the internal layers of a deformed material. The developed course of changes in the deformation of the damage factor in the case of forging in the investigated anvils renders it possible to predict the situation and the phase of deformation in which the loss of cohesion by a deformed material will occur. The comparison between the predicted and the experimental results showed a good agreement.
Utilization of Jujube Biomass to Prepare Biochar by Pyrolysis and Activation: Characterization, Adsorption Characteristics, and Mechanisms for Nitrogen
Authors: Zhang Di , Wang Tongtong , Zhi Jinhu , Zheng Qiangqing , Chen Qiling , Zhang Cong , Li Yalong
Published: December 2020

The rapid advancement of jujube industry has produced a large amount of jujube biomass waste, requiring the development of new methods for utilization of jujube resources. Herein, medium-temperature pyrolysis is employed to produce carbon materials from jujube waste in an oxygen-free environment. Ten types of jujube biochar (JB) are prepared by modifying different pyrolysis parameters, followed by physical activation. The physicochemical properties of JB are systematically characterized, and the adsorption characteristics of JB for NO3− and NH4+ are evaluated via batch adsorption experiments. Furthermore, the pyrolysis and adsorption mechanisms are discussed. The results indicate that the C content, pH, and specific surface area of JB increase with an increase in the pyrolysis temperature from 300 °C to 700 °C, whereas the O and N contents, yield, zeta potential, and total functional groups of JB decrease gradually. The pyrolysis temperature more significantly effects the biochar properties than pyrolysis time. JB affords the highest adsorption capacity for NO3− (21.17 mg·g−1) and NH4+ (30.57 mg·g−1) at 600 °C in 2 h. The Langmuir and pseudo-second-order models suitably describe the isothermal and kinetic adsorption processes, respectively. The NO3− and NH4+ adsorption mechanisms of JB may include surface adsorption, intraparticle diffusion, electrostatic interaction, and ion exchange. In addition, π–π interaction and surface complexation may also be involved in NH4+ adsorption. The pyrolysis mechanism comprises the combination of hemicellulose, cellulose, and lignin decomposition involving three stages. This study is expected to provide a theoretical and practical basis for the efficient utilization of jujube biomass to develop eco-friendly biochar and nitrogenous wastewater pollution prevention.
Analysis of Instantaneous Feedback Queue with Heterogeneous Servers
Authors: Melikov Agassi , Aliyeva Sevinj , Sztrik János
Published: December 2020

A system with heterogeneous servers, Markov Modulated Poisson flow and instantaneous feedback is studied. The primary call is serviced on a high-speed server, and after it is serviced, each call, according to the Bernoulli scheme, either leaves the system or requires re-servicing. After the completion of servicing of a call in a slow server, according to the Bernoulli scheme, it also either leaves the system or requires re-servicing. If upon arrival of a primary call the queue length of such calls exceeds a certain threshold value and the slow server is free, then the incoming primary call, according to the Bernoulli scheme, is either sent to the slow server or joins its own queue. A mathematical model of the studied system is constructed in the form of a three-dimensional Markov chain. Approximate algorithms for calculating the steady-state probabilities of the models with finite and infinite queues are proposed and their high accuracy is shown. The results of numerical experiments are presented.
Physiological Response of Corynebacterium glutamicum to Indole
Authors: Walter Tatjana , Veldmann Kareen H. , Götker Susanne , Busche Tobias , Rückert Christian , Kashkooli Arman Beyraghdar , Paulus Jannik , Cankar Katarina , Wendisch Volker F.
Published: December 2020

The aromatic heterocyclic compound indole is widely spread in nature. Due to its floral odor indole finds application in dairy, flavor, and fragrance products. Indole is an inter- and intracellular signaling molecule influencing cell division, sporulation, or virulence in some bacteria that synthesize it from tryptophan by tryptophanase. Corynebacterium glutamicum that is used for the industrial production of amino acids including tryptophan lacks tryptophanase. To test if indole is metabolized by C. glutamicum or has a regulatory role, the physiological response to indole by this bacterium was studied. As shown by RNAseq analysis, indole, which inhibited growth at low concentrations, increased expression of genes involved in the metabolism of iron, copper, and aromatic compounds. In part, this may be due to iron reduction as indole was shown to reduce Fe3+ to Fe2+ in the culture medium. Mutants with improved tolerance to indole were selected by adaptive laboratory evolution. Among the mutations identified by genome sequencing, mutations in three transcriptional regulator genes were demonstrated to be causal for increased indole tolerance. These code for the regulator of iron homeostasis DtxR, the regulator of oxidative stress response RosR, and the hitherto uncharacterized Cg3388. Gel mobility shift analysis revealed that Cg3388 binds to the intergenic region between its own gene and the iolT2-rhcM2D2 operon encoding inositol uptake system IolT2, maleylacetate reductase, and catechol 1,2-dioxygenase. Increased RNA levels of rhcM2 in a cg3388 deletion strain indicated that Cg3388 acts as repressor. Indole, hydroquinone, and 1,2,4-trihydroxybenzene may function as inducers of the iolT2-rhcM2D2 operon in vivo as they interfered with DNA binding of Cg3388 at physiological concentrations in vitro. Cg3388 was named IhtR.
Short-Term Sea Level Changes of the Upper Cretaceous Carbonates: Calibration between Palynomorphs Composition, Inorganic Geochemistry, and Stable Isotopes
Authors: Mansour Ahmed , Gentzis Thomas , Wagreich Michael , Tahoun Sameh , Elewa Ashraf
Published: December 2020

Widespread deposition of pelagic-hemipelagic sediments provide an archive for the Late Cretaceous greenhouse that triggered sea level oscillations. Global distribution of dinoflagellate cysts (dinocysts) exhibited a comparable pattern to the eustatic sea level, and thus, considered reliable indicators for sea level and sequence stratigraphic reconstructions. Highly diverse assemblage of marine palynomorphs along with elemental proxies that relate to carbonates and siliciclastics and bulk carbonate δ13C and δ18O from the Upper Cretaceous Abu Roash A Member were used to reconstruct short-term sea level oscillations in the Abu Gharadig Basin, southern Tethys. Additionally, we investigated the relationship between various palynological, elemental, and isotope geochemistry parameters and their response to sea level changes and examined the link between these sea level changes and Late Cretaceous climate. This multiproxy approach revealed that a long-term sea-level rise, interrupted by minor short-term fall, was prevalent during the Coniacian-earliest Campanian in the southern Tethys, which allowed to divide the studied succession into four complete and two incomplete 3rd order transgressive-regressive sequences. Carbon and oxygen isotopes of bulk hemipelagic carbonates were calibrated with gonyaulacoids and freshwater algae (FWA)-pteridophyte spores and results showed that positive δ13Ccarb trends were consistent, in part, with excess gonyaulacoid dinocysts and reduced FWA-spores, reinforcing a rising sea level and vice versa. A reverse pattern was shown between the δ18Ocarb and gonyaulacoid dinocysts, where negative δ18Ocarb trends were slightly consistent with enhanced gonyaulacoid content, indicating a rising sea level and vice versa. However, stable isotope trends were not in agreement with palynological calibrations at some intervals. Therefore, the isotope records can be used as reliable indicators for reconstructing changes in long-term sea level rather than short-term oscillations.