bluuebunny/crossref_metadata_2025 huggingface dataset:
IDENTIFICATION OF MUTATIONS IN THE PAH GENE IN PKU PATIENTS IN THE STATE OF MATO GROSSO
Authors: Costa Roseli Divino , Galera Bianca Borsatto , Rezende Bianca Costa , Venâncio Amanda Cristina , Galera Marcial Francis
Published: February 2020

ABSTRACT Objective: To identify phenylalanine hydroxylase (PAH) mutations in patients with phenylketonuria (PKU) from the Newborn Screening Service in Mato Grosso, Midwest Brazil. Methods: This is a cross-sectional descriptive study. The sample consisted of 19 PKU patients diagnosed by newborn screening. Molecular analysis: DNA extraction using the “salting-out” method. Detection of IVS10nt-11G>A, V388M, R261Q, R261X, R252W, and R408W mutations by the restriction fragment length polymorphism (RFLP) technique. Results: Two mutant alleles were identified in four patients (21.1%), one allele in five patients (26.2%), and none in the remaining ten patients (52.6%). A total of 13/38 alleles were detected, corresponding to 34.2% of the PAH alleles present. The most prevalent variant was V388M (13.2% of the alleles), followed by R261Q (10.1%) and IVS10nt-11G>A (7.9%). Three variants (R261X, R252W, and R408W) were not found. The most frequent mutation types were: missense mutation in eight alleles (18.4%) and splicing in four alleles (10.5%). The model proposed by Guldberg to determine a genotype/phenotype correlation was applied to four classical PKU patients with two identified mutations. In three of them, the predicted moderate/moderate or moderate PKU phenotype did not coincide with the actual diagnosis. The prediction coincided with the diagnosis of one classic PKU patient. The estimated incidence of PKU for Mato Grosso, Brazil, was 1:33,342 live births from 2003 to 2015. Conclusion: The only mutations found in the analyzed samples were the IVS10nt-11G>A, V388M, and R261Q. The genotype/phenotype correlation only occurred in four (5.3%) patients.
Is Adjuvant Chemotherapy Necessary for Patients with Deficient Mismatch Repair Gastric Cancer?—Autophagy Inhibition Matches the Mismatched
Authors: Tsai Chun-Yi , Lin Tien-An , Huang Shih-Chiang , Hsu Jun-Te , Yeh Chun-Nan , Chen Tse-Ching , Chiu Cheng-Tang , Chen Jen-Shi , Yeh Ta-Sen
Published: February 2020

Purpose

The use of microsatellite instability (MSI) and mismatch repair (MMR) as predictive biomarkers for fluorouracil-based adjuvant chemotherapy in colorectal cancer has been a paradigm shift. However, whether this applies to gastric cancer is questionable. Furthermore, we herein investigated whether and how autophagy plays a role in MSI-relevant chemoresistance.

Materials and Methods

A total of 929 patients with deficient MMR (dMMR) and proficient MMR (pMMR) gastric cancers who underwent curative-intent gastrectomy were enrolled. We compared clinicopathological variables and survival among dMMR and pMMR cohorts and tested the responses of MSI-high and microsatellite stable (MSS) gastric cancer cell lines to 5-fluorouracil (5-FU) with or without chloroquine, an autophagy inhibitor.

Results

We identified an 8.9% prevalence of dMMR cases (83 out of 929) in our cohort. This was associated with old age, tumor site at the distal stomach, an intestinal phenotype, fewer nodal metastasis, and early pathological stages. MMR was an independent prognostic factor after multivariate adjustment. Overall survival (OS) of dMMR patients was better than that of the pMMR patients but was only applicable to stage III patients. There was no difference in OS between dMMR patients treated with or without adjuvant chemotherapy, although the latter showed more medical morbidities. The MSI-high gastric cancer cell lines, versus the MSS counterparts, displayed increased resistance to 5-FU and increased autophagy. Interestingly, autophagy inhibition abrogated the chemoresistance.

Conclusion

Our data show that fluorouracil-based adjuvant chemotherapy does not work for dMMR cases, if not worse. Autophagy inhibition and/or immune checkpoint inhibition might be promising alternative strategies for gastric cancer treatment.

Implications for Practice

The use of microsatellite instability (MSI) and mismatch repair (MMR) as predictive biomarkers for adjuvant chemotherapy in colorectal cancer has caused a paradigm shift in cancer therapy, although its implications in gastric cancer are still questionable. The data obtained in the current study indicate that MSI-MMR is an independent prognostic factor for gastric cancer. Standard fluorouracil-based adjuvant chemotherapy did not work for deficient MMR cases, and was likely worse. Instead, strategies like autophagy inhibition and/or immune checkpoint inhibition should be taken into consideration in the future.
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) real-time RT-PCR N gene 2020 (Wuhan-N; 2019-nCoV-related test) -NOT RECOMMENDED v4
Authors: A Northill Judy , M Mackay Ian
Published: February 2020

NOT RECOMMENDED FOR SCREENING The sensitivity of the assay has been found to be lower than expected and we no longer recommend it be used. We do recommend the ORF1ab assay (Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) real-time RT-PCR ORF1ab 2020) or the E gene assay by Corman et al. (Protocol v2-1) A real-time RT-PCR to designed to detect SARS-CoV-2 and other related sarbecoviruses. Based on sequence MN908947 made available by Professor Yong-Zhen Zhang, Fudan University, Shanghai, China. The target region encodes the nucleocapsid (N). Not tested on wild-type virus (as of 25Jan2020), it is expected to be capable of detecting Wuhan virus, bat-like SARS and SARS virus (members of the subgenus Sarbecovirus). Limit of detection not yet determined. A single 1 mismatch at probe-binding site identified with the BetaCoV/USA/CA1/2020|EPI_ISL_406034 variant of SARS-CoV-2 (as of 29JAN2020). Probe is in the 3'-5' (reverse complement) direction. Notes: Assay is optimised (as of 24Jan2020). This test has identified a clinical positive case of coronavirus disease (COVID-19)
Sequence-Independent, Single-Primer Amplification of RNA viruses v3
Authors: Moreno Gage , O'connor David
Published: February 2020

This protocol outlines the methods to perform unbiased direct metagenomic sequencing of nucleic acid extracts from cell-free fluids. This protocol can be adapted to be run on Illumina and Nanopore sequencing platforms. The protocol is based off of the work from Kafetzopoulou et al. (PMID: 30563591). Liana has provided the lab with detailed protocols, and has worked with us extensively on optimizing and getting protocols running efficiently. Please note that this protocol has been updated to use SuperScript IV with it’s optimal temperature which has been reflected in Lewandowski et al. (DOI: https://doi.org/10.1128/JCM.00963-19) Notes: * This protocol has been used to sequence influenza direction from respiratory clinical samples (DOI: https://doi.org/10.1128/JCM.00963-19). * A team from China published last week on a 2019-nCoV familial cluster using the SISPA protocol as for coronavirus whole genome sequencing (DOI: https://doi.org/10.1016/S0140-6736(20)30154-9) SISPA-Primer A - 5'-GTT TCC CAC TGG AGG ATA-(N9)-3' SISPA-Primer B - 5′-GTT TCC CAC TGG AGG ATA-3′
Easy Holobiont Virus Extraction (Ez-HoVE) Protocol v1
Authors: Veglia Alex
Published: February 2020

Viruses are known to be both highly abundant and diverse within coral reef holobionts. Unfortunately, our knowledge of virus genomes and functional ecology within host tissue remains limited in part due to challenges associated with isolating and culturing them. To facilitate future investigations into virus genomics and ecology within marine holobionts, we present a novel virus extraction procedure that can be combined with traditional virology methods (e.g. extinction dilution or plaque purification assays) for isolation and culturing of individual virus strains from coral reef holobionts.The protocol, dubbed "Eazy Holobiont Virus Extraction" or "Ez-HoVE" protocol, described here contains five major stages: (1). Rinse and rest, (2). Tissue separation, (3). Physical cell disruption and homogenization, (4). Homogenate cleaning and (5). Virus particle concentration and validation. In short, the outline procedure allows investigators to extract and concentrate viruses from invertebrate tissue without the necessity for harsh chemicals (i.e. chloroform) or ultracentrifugation, both of which can limit investigators lacking access to large lab equipment or reagents (e.g. under budgeted institutions, isolated field stations).
Integrative and instrumental but low investment: The English learning motivation of Indonesian senior high school students
Authors: Muslim Ahmad Bukhori , Hamied Fuad Abdul , Sukyadi Didi
Published: February 2020

Considered important for global competitiveness like ASEAN Economic Society, the English proficiency of Indonesian students is unfortunately not satisfactory yet. This condition is, one of which, due to low teachers’ professionalism and students’ motivation for English learning. The motivation of students may become weak because they have not found ways to identify, drive and sustain it. Of the various studies on motivation and challenges in EFL practice in the Indonesian context, research specifically addressing how learners find motivation triggers synergized into project-based learning for sustainable and continuous English learning is still limited. This survey and interview study investigates the motivation of Senior High School (SHS) students in West Java province of Indonesia on English learning and how to drive their motivation through a collaborative project as mandated by the 2013 National Curriculum. Results of questionnaires, interviews and document analysis show that despite having integrative and instrumental motivation to learn English, the participating SHS students show low investment as indicated by low learning enthusiasm in the classroom and poor English performance. This low motivation is also related to some socioeconomic status such as school geographical location, classroom learning situation, and parental supports. The study recommends students’ involvement in a cooperative learning project which can direct and energize their English learning motivation so that they can have increased motivation, good English proficiency and become global citizens with high competitiveness.
Stress production by Cebuano learners of Arabic: A metrical analysis
Authors: Huneety Anas , Al Mashaqba Bassil , Al-Quran Majed , Mail Jehan Hishma
Published: February 2020

Stress is one of the most neglected components of the Arabic language in classrooms (Lin, 2018; Ryding, 2013).This study is devoted to analyzing stress production in Arabic as produced by Cebuano learners in order to highlight the challenges so that teachers can address them in the best way. The data have been examined within the metrical theory of word stress elaborated in Hayes (1995). A sample of 100 words has been considered, spoken by six non-native speakers of Arabic, three females and three males, whose first language is Cebuano, the national language of the Philippines. Data analysis shows that native Cebuano speakers have an iambic foot, where the foot involves left-to-right parsing, satisfies the End Rule Right Principle by which the main stress lands on the head of the rightmost visible foot, and imposes a weak ban on the degenerate foot. Intriguingly, foot iambicity observed in the produced words is regarded as a reflection of the speakers’ source language (L1) that has an iambic foot. Arabic words spoken by Cebuano non-natives conform to the bimoraic condition for the minimal phonological word that takes the primary stress, and is repaired only through vowel lengthening; whereas gemination, as a main strategy for creating bimoraicity, is totally absent. Similarly, vowel lengthening is seen as a transfer effect of L1, where stress always attracts a long vowel. The results point to the great importance of prosody in teaching Arabic as a foreign language, since prosodic features significantly contribute to the communication intelligibility.
Multimodal literacy: Unfolding reading path in children’s picture book and its potential for EFL classrooms
Authors: Damayanti Ika Lestari , Febrianti Yusnita
Published: February 2020

This paper reports on an investigation into the reading path patterns in a children’s picture book. The primary investigation focused on the semiotic resources involved in the construction of meanings in picture books i.e. language and image elements. These semiotic resources provide insights into the unfolding of the storylines in the observed picture book. In order to detail the roles of these resources, a picture book entitled Tacky the Penguin (Lester, 1988) was selected and examined by employing two methods: Kress and Van Leeuwen’s (1996, 2006) structure of information value and Royce’s (1998) Intersemiotic Complementarity. The results of the analysis suggest that the whole picture book is built from a page-by-page reading path that allows for a linear and non-linear reading path. While each page presents a different layout of information value, the general pattern leads to a linear reading path from the beginning up to the end of the picture book. The mapping of the reading path is reinforced by the interrelated meanings between verbal and visual resources on the page. Drawing on the analysis results, this paper offers the delve into the flow of information from the language and image resources in the picture books that can be used to inform practical use of picture books in EFL classrooms.
KNOWLEDGE CREATION AND INNOVATIVE BEHAVIOR
Authors: Setiawan Yana , Yuniarsih Tjutju , Suwatno Suwatno , Ahman Eeng
Published: February 2020

The current business development is entering the industry era 4.0. To anticipate the development of the industry era 4.0, employee performance aspect in human resource management becomes very important. Problems in this research is the low innovative behavior of employees described by the phenomenon of decline and increase performance of employee performance PT. Perkebunan Nusantara VIII (PTPN VIII) in Industrial era 4.0 in Indonesia. If the human resource problem is not addressed immediately, it will have an impact on the achievement of corporate management objectives which in turn also affect the performance, productivity, competitiveness and sustainability of the company. The purpose of this study is to describe and analyze employee perceptions on (1) the effectiveness of knowledge creation and the level of innovative behavior (2) Effect of the effectiveness of knowledge creation on the level of innovative behavior. The research method used a quantitative approach that is descriptive and verification through descriptive survey and explanatory survey. Respondents and research population are employees of PTPN VIII as many as 3999 people, with sample size of 364 people. The data analysis technique used Structural Equation Modeling (SEM) with SPSS and AMOS 24 software tools. The findings of the research show that (1) Knowledge creation is in effective category and innovative behavior is in high category. (2) Knowledge creation has a positive and significant effect on innovative behavior
Ecological Monitoring Of Small Water Systems: Algorithm, Software Package, The Results Of Application To The Uzh River Basin (Ukraine)
Authors: Коtsiuba I.G. , Skyba G.V. , Skuratovskaya I.A. , Lyko S.M.
Published: February 2020

A systematic approach to environmental monitoring of small water systems and its optimization is proposed. For simplify the storage of large amounts of information, accelerate the obtaining of preliminary conclusions of water resources condition, predict the dynamics of both individual indicators and their system attributes, and develop a user-friendly interface, which is tied to the area. To carry out the research, samples of water were taken near the corresponding sections of industrial enterprises of Korosten region, as well as discharges of a storm water system. Based on the standard approach, with the help of physicochemical methods of analysis practically all the hydrochemical parameters in the studied water body were determined. The obtained results were compared with the regulatory guidelines. For systematization and maintenance of environmental monitoring, a software package has been developed, which has been tested on the results of the instrumental laboratory analysis of surface water quality of the river Uzh. The software package is developed to establish local sources of pollution, monitor potentially dangerous man-made objects, and better manage of water resources. A user-friendly interface has been developed, which is tied to the area and is easily adapted to other monitoring objects. Thanks to the developed software package, the implementation of an integrated approach to the collection and analysis of monitoring data is carried out quickly, efficiently and conveniently. The developed approach allows easy adaptation to the objects of the research and does not require significant capital investment.
The role of nimodipine and magnesium sulfate in the prevention and treatment of vascular spasm in patients in the acute rupture of cerebral aneurysms
Authors: Kalinkin Aleksandr A. , Vinokurov A. G. , Kalinkina O. N. , Yusubalieva G. М. , Chupalenkov S. M.
Published: February 2020

Vascular spasm in patients with hemorrhage from rupture of cerebral aneurysms is the main cause of adverse outcomes of the disease. One way to treat persistent contraction of cerebral arteries is to use nimodipine and magnesium sulfate. This literature review presents studies on the use of nimodipine and magnesium sulfate in the treatment of vascular spasm, and highlights the main links of pathogenesis and drug action mechanisms.
Post-Geleneksellikten Post-Modernizme İki Papanın Hikayesi
Authors: YEL Ali Murat
Published: February 2020

İşte çok kısa bir şekilde özetlenebilecek yenilik, modernlik, gelenek, güncelleme ve çağa ayak uydurma gibi bilinen tartışmaların gölgesinde bu zıt gibi görünen iki tavrın aynı zaman diliminde iki ayrı otorite tarafından nasıl savunulacağı ve “uzlaşma” sağlanıp sağlanamayacağı sorusuna cevap arayan bir sinema yapımı gösterime girdi. Aralık ayının sonlarında Netflix platformu üzerinden vizyona giren The Two Popes/İki Papa (Fernando Meirelles, 2019) filmi de Katolik Kilisesi’nin asırlardır süren gelenek, reform veya günümüz dünyasına ayak uydurabilme tartışmalarının odağındaki iki papanın seçim-istifa-seçim yoluyla birbirlerinin yerine nasıl geçtiklerini anlatmaktadır.
Гiсторыка-культурны аспект намiнацыi урбанонiмаў сучаснага горада Брэста
Authors: Famielec Monika
Published: February 2020

Miasto Brześć, którego historia liczy tysiąc lat, wchodziło w skład Rusi Kijowskiej, Wielkiego Księstwa Litewskiego, Rzeczypospolitej, a od roku 1795 – Imperium Rosyjskiego. W XX w. miasto było częścią Polski (1919–1939), wchodziło w skład Związku Radzieckiego (1939–1941) jako centrum nowo utworzonego obwodu brzeskiego Białoruskiej Sowieckiej Socjalistycznej Republiki (BSSR), znajdowało się pod niemiecką okupacją (1941–1944), ponownie znalazło się w granicach ZSSR (1944–1991, od roku 1945 – BSSR), a od 1991 roku należy do suwerennej Republiki Białoruskiej. Niniejszy artykuł poświęcony jest historyczno-kulturowemu aspektowi nazewnictwa ulic współczesnego miasta Brześcia. Analizie semantycznej poddanych zostało 51 jednostek powstałych w XXI w. Zaprezentowany materiał pokazuje, że w procesie nominacji kontynuowany jest, często stosowany w XX w., model nadawania ulicom nazw pamiątkowych, służący upamiętnieniu postaci zasłużonych dla miasta Brześcia i dla obwodu brzeskiego w czasie działań wojennych w latach 1941–1944 (J. M. Winnik, W. P. Puganow, M. J. Krisztofowicz), jak również upamiętnieniu znaczących dla miasta wydarzenie historycznych, jakim była Bitwa pod Grunwaldem w roku 1410. Najnowsze ulice zostały też nazwane na cześć osób, których losy związane były z miastem przez miejsce zamieszkania bądź też inną działalność na rzecz rozwoju powojennego Brześcia (W. D. Machnowicz, W. I. Goździeckij). Jest też grupa nazw ulic upamiętniających nazwiska mistrzów pióra: białoruskich (N. Zasim, W. A. Kolesnik, I. P. Mieleż), rosyjskich (S. A. Jesienin, A. Płatonow, A. S. Gribojedow) i polskiego pisarza, historyka i działacza społecznego (J. U. Niemcewicza). Nazwy ulic nadane w pierwszym dwudziestoleciu XXI wieku, utrwalają ponadto nazwy obiektów sakralnych (ulica Iljinskaja, Irininskaja, Pokrowskaja) bądź bezpośrednio nawiązują do usytuowanej na ulicy budowli kultu religijnego (ulica Gieorgijewskaja, Rożdiestwienskaja, Sierafima Sarowskogo). Po raz pierwszy na mapie miasta pojawiły się nazwy motywowane imionami świętych Cerkwi prawosławnej (Borys, Włodzimierz, Tatiana). Próba odrodzenia języka białoruskiego w roku 1991 doprowadziła do tego, że nazwy urzędowe były zapisywane w języku białoruskim i rosyjskim. Najnowszy spis brzeskich ulic wzbogacił się o jedną nazwę pochodzenia białoruskiego – Starożytnaja ulica (biał. старажытны – ros. древний, pol. starożytny). Oddaje to charakter miasta, świadka skomplikowanej historii na przestrzeni wieków.
Paz y democracia: una aproximación al concepto de paz y refrendación democrática
Authors: Jiménez Ramírez Milton César , Arboleda Ramírez Paulo Bernardo
Published: February 2020

This study presents an analysis of the scope of the concept of peace, especiallyas a political process and about who definitively should decide on the approval of a peaceprocess, for which, the Colombian case is taken as reference. The article is developed froma legal approach in two parts. The first part, examines the main elements of the concept ofpeace within the framework of a social rule of law; the second explores the role of judicialreview and democracy (popular or legislative) in approving or not a peace agreement, as wellas postulating who is entitled to adopt these decisions. Finally, a brief conclusion is givenabout the need for a primacy of popular democratic practice. To this end, a documentarybibliographical method is used, as well as a descriptive analytic, with a qualitative andhermeneutical approach.
Guía para la caracterización de la víctima policial en Colombia: visibilización del policía y su familia, víctimas del conflicto armado en Colombia
Authors: Suárez Mendoza Angélica del Pilar
Published: February 2020

The present document materializes in a simple way an approach to thecharacterization activity for the policemen and their families as victims of the armedconflict, and integrates the definition of victim of the armed conflict in people conformingthe National Police.Likewise, all victims without exception and with special emphasis on this differentialapproach require truth, justice (whether judicial or extrajudicial), integral reparation andguarantees of non-repetition. The reparation of the victims of the armed conflict in Colombiamust be comprehensive in the sense indicated by the Constitutional Court in explaining theprinciple of equality from a differential approach, recognized in Law 1448 of 2011, all seenfrom a clear concept of the armed conflict, which can be recognized as political, legal andsociological that give rise to different forms of explanation through different professionsand their points of view (Mejia J. C., 2013).
Problematización y eventualización como métodos para el análisis del miedo y sus usos políticos
Authors: Agudelo López Alexandra
Published: February 2020

The article resulting from the doctoral research Fobopolítica, rubrics of acontemporary governmentality developed in Mexico and Colombia (2012-2016), proposesthe analytical tools of eventualization and problematization proposed by Michel Foucault, asa method for the analysis of the political use of fear. This method allows to understand thetransformations of the contemporary State towards extremely punitive and control models,while illustrating the ways in which other actors can use fear for purposes of sovereignty,territorial appropriation and domination over the population. In the field of law, thesemethods are a possibility to understand the reformist tendency of the legal and normativespheres and the effects on the protection and guarantee of civil and political rights
¿Las mujeres son más sensibles frente al maltrato animal? Experiencias entorno a la aplicación de la Ley 1774 desde 2016 hasta 2018
Authors: Pérez Rodríguez Adriana Marcela , García Flórez Cristian Matheo , Pérez Rodríguez Jose Fernando
Published: February 2020

The present investigation tries to give a justification to the apparent greaterinterest that women have for the animal abuse cases in Colombia. By means of the statisticalanalysis of the information obtained from the users of the Legal Office for the Protection ofNon-Human Animals -Abogato-, and based on the theoretical reflections they make in thecontext of ‘moral sensitivity’.All this job done by by Peter Strawson and Ernst Tugendhat
Análisis ético y político criminal de la Ley 1774 de 2016
Authors: Sanguino Cuellar Kenny Dave , Peláez Mejía José María
Published: February 2020

Taking into account the Law 1774 of 2016 on the protection of animals, it ispertinent to analyze the ethical foundations that support this regulation, what are theimplications in the field of criminal law and dogmatics when assuming animals as a legalright and the consequences in political-criminal matters when creating a new crime forthose who seriously mistreat these beings.
El carácter histórico de la Ley 1774 de 2016
Authors: Pacheco Niño Diego Javier
Published: February 2020

The present article focuses on the historical evolution of animal protection fromthe cultural and juridical level showing its eventual importance in a social and democraticstate of law through criminal law. This research might eventually lead to position the lawas an articulation of the political action of animal movements. Therefore, this work aimsto denote the impact that a legal provision can generate in the perception and awarenessof an other such as the non-human animal.
La objeción de conciencia frente a la interrupción voluntaria del embarazo en Colombia
Authors: Salazar Medina William Javier , Medina Rico Ricardo Hernán
Published: February 2020

This article analyzes the regulations and jurisprudence regarding conscientiousobjection, the legal authorization for the voluntary termination of pregnancy and theirrelationship. It focuses on the development and evolution that these rules have had asguarantors of the rights to freedom of conscience and sexual freedom, as well as thereproductive self-determination of women and, as a result, possible solutions are shownin the face of conflict events or conflict between the rights, when the medical and sanitarypersonnel refuse to carry out the porcedures protecting their own religious beliefs or theirmoral principles.As a methodology, a qualitative research was developed based on information obtainedfrom the jurisprudential, doctrinal analysis and interviews. This allowed a description,classification, explanation and interpretation of data, as well as interviews. It was possibleto determine the current situation of the causes under which the practice of voluntaryinterruption of pregnancy is legal and the possible events of moral objection by medicalpersonnel. Likewise, it was concluded that whenever the medical procedure is requestedwithin the legal parameters, the provision of the service must be ensured. Likewise, theright to object to the practice of the voluntary interruption of pregnancy is personal andcan never be claimed as an institutional policy.
Marco jurídico para la autonomía económica de las mujeres en Colombia
Authors: Goyes Moreno Isabel
Published: February 2020

This article is one of the products of the research “Economic Autonomy of Womenin Nariño”, developed by the Nariño Gender Observatory. This document refers to one ofits results: The international and national legal basis on the autonomy and economic rightsof women. Of all the violence (sexual, physical, psychological) that affect their lives (whosecauses are remote and perhaps invisible), economic violence is one of the most relevantfor its effects both in the family, as in the construction of identity and female citizenship.This problem has been a matter of Declarations, agreements and universal Conventions;however, women’s lives are very distant from such mandates. For this reason, the Stateshave committed themselves to the realization of these rights closely linked to the principlesof equality, non-discrimination and human dignity.From the CEDAW to the SDGs, gender equality was incorporated into official agendas,admitting that without this condition it is not possible to overcome poverty or boost theeconomy. Colombia, on the other hand, acquired commitments with the achievement ofinternational standards on the economic autonomy of women. Contributing to promotethese achievements is the purpose of this writing.
La pensión de sobrevivientes para parejas del mismo sexo en Colombia
Authors: Patiño Jiménez Jessica Alejandra , García Juan Laura
Published: February 2020

This research approaches the concept of family in the Colombian legislationand its treatment in the Constitutional Court jurisprudence, focusing on the study of therights of homosexual couples. The work focuses on the survivor’s pension and other issuesrelated to Social Security, and analyzes the regulations and jurisprudential backgroundcomparing them with the current legislative framework in Colombia. Taking into accountthat International Law of Human Rights plays an essential role in the legal formulationsand judicial decisions in this matter, the investigation reviews the ruling of the case Duquevs. Colombian in 2016, solved by the Inter-American Court of Human Rights. One of theconclusions is that this country continues to violate its international commitments andignoring the jurisprudence of the High Courts when the government and justice continueto deny the right to a survivor’s pension claimed by homosexuals.
Presunción de inmuebles baldíos carentes de antecedentes registrales y de titulares de derechos reales
Authors: Gonzalez Flórez Flor Margoth
Published: February 2020

The theories of the Constitutional Court and the Supreme Court of Justice are explained to determine the legal nature of real estate that does not have a record or real rights, in respect of which the declaration of belonging is requested. The work concludes that the legal regime of vacant property in Colombia, with Law 160 of 1994, corresponds to the model of “adjudication title”, and consequently, contrary to what is established in Law 200 of 1936, the presumption was stipulated of domain of the State. Therefore, when the property does not have a record, the judge is obliged to link and request the National Land Agency to carry out the process of clarification of the property.
Consumo de drogas en Colombia: análisis del enfoque de salud pública para su abordaje
Authors: Gómez Acosta Andrés , Sierra Barón Willian , Rincón Perdomo Julieth Milena
Published: February 2020

Although the Congress of the Republic of Colombia for several years now recognizes theneed to address the problem of drug use as a disease that requires comprehensive care,that is why Law 1566 came in 2012, it is still necessary to establisha national policy thatguarantees its approach from a public health perspective, which not only highlights thecontrols related to production and marketing (supply reduction), but also strengthens boththe efforts associated with specialized clinical care, as those interventions emphasized toreduce those factors that underlie the beginning, maintenance and relapse to consumption(demand reduction). This document aims to synthesize the recent evidence referred byexperts and institutions related to the conception and approach of the drug issue, whichwill lead to establish actions to reduce demand and supply within the framework of thepublic health approach.
La consolidación de la reparación integral en virtud de la aplicación del control de convencionalidad por el juez contencioso administrativo
Authors: Camacho Vinueza Daniela , Cárdenas Caycedo Omar Alfonso
Published: February 2020

Human rights have been conceived as values, principles and real guaranteesfor individuals, thereby marking different guidelines in the internal legal systems ofthe States and being configured as a strict parameter for national judges. In this way,the materialization of the provisions contained in the American Convention on HumanRights, has occurred in the decisions of the internal judges of the different States Partiesto this international treaty, deriving this activity to the application of the so-calledcontrol of conventionality. In this scenario, it is interesting to analyze the behavior thatthe contentious administrative judge has had regarding the application of the diffusecontrol of conventionality for the recognition of the integral reparation of the victims ofthe Colombian armed conflict, understood this repair as the proportional recognition ofpecuniary measures and non-pecuniary reparation, in order to guarantee the fundamentaland human rights of the victims who have been violated through actions or omissions ofthe Colombian State.
La naturaleza pública del contratista como excepción a las reglas de publicidad y de competencia en un contrato público: Una mirada al derecho francés y al derecho colombiano
Authors: Rincón Jaimes Andrea Esther
Published: February 2020

Both in France and in Colombia, the contractual action of the Administration is directed by a series of rules and principles to select the contractor with the most favorable offer in the case of Colombia, or the economically most advantageous offer in the French case. Contracts subject to these types of rules are called state contracts in Colombia, and contracts of the commande publish in France.The legal system of each country admits the participation of subjects of public law as possible contractors of a state contract or of a contract of the commande publique; However, it is possible to observe how the Colombian legal system, unlike French law, grants these subjects of public law the possibility of being selected under the direct contracting procedure, a situation that is viable thanks to its public nature and compliance of certain requirements established by law. This idea is inconceivable in the French legal system, in which the subjects of public law who claim to be holders of a contract of the commande publique are qualified as economic operators, so allowing the conclusion of the contract without competition and publicity rules.IT constitutes a preferential treatment that would violate European principles such as transparency and non-discrimination
Los títulos-valores electrónicos en el Ordenamiento Jurídico Colombiano
Authors: Mayorga Penna Pablo Andrés
Published: February 2020

With the promulgation of Law 599 of 1999, the foundations of electronic commerce were established and, giving efficiency, validity and probative force to data messages, the discussion of electronic securities emerged under the principle of functional equivalence; and although the previous regulations allowed to comply with the essential requirements of securities in the digital scenario, it became insufficient to guarantee, under unique criteria, the circulation of these intangible exchange documents. Nowadays, in response to the gaps in the previous regulations, a legislative proposal that expressly and specially intends to transplant the centralized method of the stock market to these immaterial documents has been enforced, requiring that electronic securities be guarded and / or managed by centralized securities deposits, who will be responsible for issuing a certificate for the purposes of exercising the rights incorporated. Without prejudice to the benefits that the previous proposal for the circulation of the titles registered in a centralized database may report, also guaranteeing the traceability of the operations and encumbrances on the intangible document, it will be concluded that the above breaks with the essential principles of the securities and the consequences derived from it will be exposed. Despite the dogmatic analysis contained in this article, the adequate conceptualization of the intangible titles that use the centralized mechanism exposed will be of great importance for purposes of establishing a regulation more appropriate to their own characteristics, being respectful of the scientific method that should reign in law.
Las Técnicas de Reproducción Humana Asistida. Propuesta de lege ferenda en el ordenamiento jurídico cubano
Authors: González Cruz Claudia , Morffi Collado Claudia Lorena
Published: February 2020

The present work entitled “Assisted Human Reproduction Techniques. Proposal of lege ferenda in the Cuban legal system”, brings together some legal reflections regarding the application and development of assisted human reproduction techniques and their repercussion for the Law. Assisted human reproduction techniques consist of the manipulation of gametes and embryos through various procedures, the most common being artificial insemination, in vitro fertilization and intracytoplasmic microinjection. Its application displays ethical, moral, psychological and legal repercussions, establishing differences in its treatment. Law as a social creation is not immutable or removable, but temporary, variable and dynamic since it must adapt to all social, cultural, political, economic and scientific changes; However, over time it is perceived that the Law does not run at the same pace of science. Due to the results implemented, adjusting the legal system to the development achieved by the application of assisted human reproduction techniques, through the study of the dissimilar legal problems that it causes, such as the determination of maternal and / or paternal affiliation, constitutes a pressing need in the current Cuban law system. In Cuba, like several countries in South America, there is no legislation in this regard, so the formulation of a special law designed to regulate the application and development of techniques is a pending task in the legislative improvement of Cuban law. The general objective is to base the need for legal regulation of assisted human reproduction techniques in the Cuban legal system.
Quantification of C-reactive protein, differential count and blood glucose in acute coronary syndrome
Authors: Padmanaban U. B. , . Lenin , Kumar G. Sathish
Published: February 2020

Background: The study was done to investigate whether the raised levels of C-reactive protein (CRP), differential count and random blood glucose, besides echocardiogram, enhances the assessment process of acute coronary syndrome (ACS).Methods: This prospective study was done on 100 patients with typical chest pain attending to the department of medicine at K.A.P.V Medical College and Hospital, Trichy during the period from 2015 to 2017. The serum was assayed on admission for CRP, differential count and random blood glucose. Correlation of these parameters with incidence of ACS was calculated.Results: Male preponderance was seen in the study. Out of 100 patients, majority of about 60% of ACS patients had raised JVP. Elevated level of CRP was seen in 73% patients, 70% had elevated level of ejection fraction percentage. 71% had elevated level of WBC and 58% had elevated level of RBS. Statistically significant correlation was observed with the level of CRP (p=0.044), differential WBC count (p=0.037) and random blood glucose levels (p=0.001).Conclusions: Our study indicates that elevated CRP levels, increased random blood sugars and leucocytosis in ACS patient are positively correlated with decreased ejection fraction. Hence, measuring the levels of these parameters will helps in identifying incidence of acute coronary syndrome without echocardiogram.
Comparison of clinicopathological characteristics of endogenous and exogenous cervical cancer with its clinical significance
Authors: Choudhry Fariha , Muhammad Tahir , You Xuewu , Liu Lu , Abdulaziz Asrar M. A. , Sun Yu , Cui Baoxia , Han Sai , Zhang Youzhong
Published: February 2020

Background: Cervical cancer is the third leading cause of cancer death in women of less developed countries due to poor screening and decreased diagnostic approaches. We aimed to investigate and differentiate the distinct clinicopathological characteristics and prevalence of endogenous and exogenous cervical in hospitalized patients.

Methods: A study was performed contained 663 patients that were enrolled and underwent for screening of endogenous and exogenous cervical cancer in Qilu Hospital of Shandong University, from January 2010 to March 2015.  

Results: Our results indicated that combined examination of thin-prep liquid-based cytology test and human papillomavirus (HPV)-DNA are effective for diagnosis of endogenous and exogenous cervical cancer. The clinicopathological characteristics based on tumor growth pattern, and high risk HPV-DNA incidence had no significant difference (p>0.05) in endogenous and exogenous cervical cancer patients. The higher ratio of lymph node metastasis in endogenous cervical cancer and exogenous cervical cancer during IB1-stage (24.3% vs 12.9%), and in IIA2-stage (36.4% vs 25%) was observed respectively. In addition, our data provide compelling evidence that the level of deep interstitial infiltration, and lymphatic vascular infiltration in endogenous cervical cancer was collectively higher (82.7% and 33%) compared to exogenous cervical cancer (62.4% and 18.3%) respectively during all stages.

Conclusions: The higher percentage of lymph node metastasis, deep interstitial infiltration, and lymphatic vascular infiltration was observed in endogenous cervical cancer which might be the biomarker and differential key points for the diagnosis of endogenous cervical cancer. Taken together, our study provides clinicopathological features to diagnose, and differentiate the endogenous and exogenous type cervical cancer with its prevalence.
Quadriceps-splitting midline approach in the treatment of distal femur infected nonuinon with stiff knee and severely scarred soft tissues
Authors: Bliss Jeremy , Inja Dan Barnabas , Nithyananth Manasseh , Cherian Vinoo Mathew
Published: February 2020

Background: Treatment of infected distal femur non-union with a stiff knee and severely scarred soft tissues is a challenging problem. We describe a method of addressing the non-union using quadriceps splitting approach to the distal femur.

Methods: We retrospectively reviewed 5 patients with distal femur infected nonunion and knee stiffness, who, after infection control, required distal femur bone grafting. All patients had autogenous iliac crest bone grafting of the distal femur using the quadriceps splitting approach. The parameters assessed were the time to surgical wound healing, wound infection, time to bony union, and if any additional procedures were needed.

Results: 5 patients were referred with distal femur infected non-union in addition to knee stiffness, with or without an implant in situ. All patients underwent debridement, implant exit, and external fixation of the femur spanning the knee as the primary surgery here, followed later on by iliac crest bone grafting of the distal femur using the above approach. All patients united well within 12 to 16 weeks, without the need for additional procedures.

Conclusions: In the presence of pre-existing knee stiffness with severely scarred and contracted soft tissues the quadriceps-splitting approach is a useful method to address bony problems in the distal femur, without the need for a separate procedure for soft tissue or flap cover.
Clinical and functional outcomes of pre-contoured plate fixation in displaced intraarticular calcaneum fracture
Authors: Kumar Babu B. L. S. , Bhavanasi Bhaskar Amarnath , Reddy Narreddy Jayasomeswar
Published: February 2020

Background: We evaluated the clinical and functional outcome of intra-articular calcaneal fractures treated with pre-contoured plating. The functional outcome was measured by the American Orthopaedic Foot and Ankle Society (AOFAS) scoring system.

Methods: Twenty-five adult patients with displaced intra-articular calcaneus fractures, treated with by open reduction and internal fixation using pre-contoured plates, and those who were available for follow up were evaluated for the outcomes such as operative procedure, anatomical reduction, postoperative hospital stays early mobilization and functional outcome postoperatively. Follow-up was at three, six and twelve months radiological and clinical union were also considered.

Results: Twenty-five adult patients presented with calcaneal fractures were operated during the study period. The mean time for union of fracture was 12.5±1.8 weeks. When we assessed the functional outcome in those patients who had radiological and clinical union using AOFAS score, we found that 16 out of 23 (64%) had good results with mean AOFAS score of 83.5±2.0 and 7/25 (28%) had fair results with mean score of 73.3±1.3, and 2/25 (8%) had poor results with mean score of 54.0±8.5. In 2 out of 16 (12.5%) patients, with good AOFAS scores, implants were removed after one and a half year from the day of surgery after confirming complete union of the fracture.

Conclusions: We believe that pre-contoured reconstruction plate fixation for displaced intraarticular calcaneus fracture is excellent in terms of the union of the fracture, attaining anatomical reduction. It gives better outcomes concerning the function and range of movements.
Reflections on my role as a mental health service user co-applicant in a randomized control trial
Authors: Lea Laura , Byford Sarah , Coney Yve , Crane Rebecca , Fagabemi Natalia , Gurney Tony , Leigh-Phippard Helen , Rosten Claire , Simms Kate , Strauss Clara
Published: February 2020

This is not a research paper but a personal and collective reflection of patient and public involvement (PPI) for the LIGHTMind 2 randomized control trial (www. isrctn. com/ISRCTN13495752). This trial compares two guided self-help psychological interventions for depression, and is delivered
in the UK NHS Improving Access to Psychological Therapy services. The paper is the result of my reviewing our PPI 18 months into the trial. The PPI includes myself as a research team member and co-applicant, with lived experience of depression, mindfulness and cognitive behaviour therapy.
There is a Lived Experience Advisory Panel of six people with lived experience of depression or mindfulness, who advise the researchers. Two people with lived experience of mental health difficulties and knowledge of PPI attend the Trial Steering Committee. This paper includes comments from
some of the other people with lived experience and from researchers involved in the trial, included as co-authors. I offer the Johari window (Luft, 1970) and the 4Pi National Involvement Standards (NSUN, 2018) as a way of positioning the value of PPI. Developing relationships within PPI is
identified as a way of moderating the fear that some people experience as they work with researchers. I describe the importance of principles that incorporate explicit statements about the value of PPI.
Diseño y cultura. Huellas japonesas en la Argentina
Authors: Veneziani Marcia
Published: February 2020

Dado el creciente interés por la cultura japonesa en la Argentina, en el presente ensayo se intenta analizar el vínculo entre los conceptos de instante, vacío, ambigüedad y vulnerabilidad, para relacionarlos con el diseño, el arte, la estética, la filosofía Zen y la naturaleza. El kimono, la puerta característica denominada shōji, sus marcos de madera y papel washi, la poesía Haiku, el blanco y las sombras, son algunos de los ejemplos que se abordan para explicarlo. Resulta esencial además, comprender que también el lenguaje japonés, está indisolublemente ligado al diseño y a su filosofía. Para un japonés hablar y escuchar es un verdadero arte. Es por ello que el escrito aborda la cuestión a partir, no solo de los textos de Kenya Hara, Junichirō Tanizaki y de Jorge Luis Borges, sino que además incluye -necesariamente- un diálogo entre la autora del presente artículo y la filósofa nipona Mami Goda, residente en la Argentina desde hace casi una década.