bluuebunny/crossref_metadata_2025 huggingface dataset:
Animal Slurry Sanitization through pH Adjustment: Process Optimization and Impact on Slurry Characteristics
Authors: Rodrigues Joana , Alvarenga Paula , Silva Ana Carla , Brito Luísa , Tavares Jorge , Fangueiro David
Published: March 2021

Sanitization by pH adjustment of dairy and pig slurries was evaluated for potential use as organic fertilizer in horticulture. This requires absence of Salmonella in 25 g of slurry and less than 1000 Escherichi coli colony-forming unit per gram of fresh slurry (Regulation (EU) 2019/1009). Additives used in the alkalinization and acidification treatments, included hydroxide-salts and nitrogen-based reactants to increase slurry pH to a basic range (9.0–11.0) and concentrated H2SO4 to decrease slurry pH to an acidic range (5.5–3.5). While low-cost urea was unable to increase the slurry pH above 9.5, ammonia efficiently increased slurry pH to the targeted values (but enhanced the emissions risk), whereas the effect of Ca(OH)2 was hindered by its low solubility. Slurry sanitization by alkalinization was achieved at a pH of 9.5 for both slurries, using similar quantities of KOH or NaOH. KOH was selected for further tests since it provides a plant macronutrient. Acidification with concentrated H2SO4 was able to achieve sanitization by lowering the pH to 5.0. After a 60-d storage experiment with raw and treated slurries, the level of E. coli was below the sanitization limit for all samples. Storage had no significant impact on slurry characteristics, except for ammonium-nitrogen content. Acidification treatment minimized ammonia losses.
Effects of Fertilizers and Manures on Temporal Yield Variability of Winter Rye
Authors: Studnicki Marcin , Macholdt Janna , Macdonald Andy , Stępień Wojciech
Published: March 2021

The anticipated increases in environmental variability associated with climate change may lead to enhanced abiotic plant stresses (e.g., heat stress, drought stress, etc.) resulting in greater inter-annual yield fluctuations and higher crop production risk. While there has been increasing attention to adaptation measures, there is little evidence available on how to change agronomic management strategies to maintain stable yields in winter rye production systems in Poland. This study uses rye yields from the unique Skierniewice Long-term experiment (Poland) to examine for the first-time the long-term effects of different nutrient regimes on crop yield stability from 1966 to 2015. Yields from six combinations of mineral fertilizers and lime (CaNPK, NPK, CaPK, CaNK, CaNP, Ca), with and without additional manure, were used to estimate the temporal yield variability of winter rye. A novel statistical approach based on a mixed model approach with REML (restricted maximum likelihood) stability parameter estimation was used. The results showed that the use of additional manure in ‘sub-optimal’ mineral fertilizer treatments, such as Ca and CaPK (without mineral N), reduced the temporal yield variability of rye. In contrast, additional organic input led to more variable rye yields in already ‘optimal’ treatments including mineral N (CaNPK and NPK), compared to those with no additional manure. Winter rye given CaNPK and NPK, without additional organic manure demonstrated high yield and low temporal yield variability. In contrast, yields of treatments with no mineral N (Ca and CaPK) and no additional manure supply were low and unstable. In addition, it was found that increasing soil organic carbon resulted in larger, more stable yields. These findings highlight the importance of ensuring rye crops receive sufficient fertilizer to maintain crop production levels and yield stability, especially in dry years. They also demonstrate the importance of avoiding the excessive use of organic manures when fertilizer inputs are sufficient to meet crop demand. Overall, the study provides novel insights about how to maintain grain yields and minimize temporal yield variation of rye in arable cropping systems, which will become increasingly important in a changing climate in Poland and in other temperate climate areas. This study also highlights the importance of soil organic carbon for improving the climate resilience of winter rye, while simultaneously meeting the demand for more sustainable management of the soil.
Seeds Quality and Quantity of Soybean [Glycine max (L.) Merr.] Cultivars in Response to Cold Stress
Authors: Staniak Mariola , Stępień-Warda Anna , Czopek Katarzyna , Kocira Anna , Baca Edyta
Published: March 2021

The aim of the study was to identify the response to cold stress of 16 soybean cultivars by evaluating their emergence, yield level, and seed chemical composition. Studies were conducted in 2018–2019. A total of sixteen soybean cultivars belonging to three earliness groups (early, medium-early and late) were included. Short-term (3-day) cold stress (12/6 °C day/night) was applied immediately after sowing (A), 3 days (B) and 6 days (C) after sowing seeds, while long (9-day) cold stress (D) was applied immediately after sowing seeds. In the control plot (K), plants were grown under optimum conditions (20/15 °C day/night). The study showed that cold stress, reduced plant emergence by 5–10%, depending on the treatment. Long stress (D) had a beneficial effect on the yield of all soybean cultivars (average yield increase of 21.5%), with statistically significant differences in 12 cultivars and a trend in four cultivars. Short stress also caused a significant increase in yield on treatments B and C (by 6.8 and 11.6%, respectively). Cold stress did not significantly affect the nutrient content of seed yield. Varietal differentiation was found with respect to yield and chemical composition of seeds.
Predicting the Digestive Tract Development and Growth Performance of Goat Kids Using Sigmoidal Models
Authors: Abdelsattar Mahmoud , Zhuang Yimin , Cui Kai , Bi Yanliang , Zhang Naifeng
Published: March 2021

The transition from monogastric to rumination stage is crucial in ruminants’ growth to avoid stressors—weaning and neonatal mortalities. Poor growth of the digestive tract could adversely affect the performance of the animal. Modeling informative growth curves is of great importance for a better understanding of the effective development pattern, in order to optimize feeding management system, and to achieve more production efficiency. However, little is known about the digestive tract growth curves. For this reason, one big goat farm of Laiwu black breed was chosen as a basis of this study. Forty-eight kids belonging to eight-time points (1, 7, 14, 28, 42, 56, 70, and 84 d; 6 kids for each) were selected and slaughtered. The body weight, body size indices, rumen pH, and stomach parts were determined and fitted to the polynomial and sigmoidal models. In terms of goodness of fit criteria, the Gompertz model was the best model for body weight, body oblique length, tube, and rumen weight. Moreover, the Logistic model was the best model for carcass weight, body height, and chest circumference. In addition, the Quadratic model showed the best fit for dressing percentage, omasum weight, abomasum weight, and rumen volume. Moreover, the cubic model best fitted the ruminal pH and reticulum percentage. The Weibull model was the best model for the reticulum weight and omasum percentage, while the MMF model was the best model describing the growth of chest depth, rumen percentage, and abomasum percentage. The model parameters, R squared, inflection points, area under curve varied among the different dependent variables. The Pearson correlation showed that the digestive tract development was more correlated with age than body weight, but the other variables were more correlated with body weight than age. The study demonstrated the use of empirical sigmoidal and polynomial models to predict growth rates of the digestive tract at relevant age efficiently.
Effects of Increasing Doses of Condensed Tannins Extract from Cistus ladanifer L. on In Vitro Ruminal Fermentation and Biohydrogenation
Authors: Guerreiro Olinda , Alves Susana P. , Costa Mónica , Duarte Maria F. , Jerónimo Eliana , Bessa Rui J. B.
Published: March 2021

Cistus ladanifer (rockrose) is a perennial shrub quite abundant in the Mediterranean region, and it is a rich source in secondary compounds such as condensed tannins (CTs). Condensed tannins from C. ladanifer were able to change the ruminal biohydrogenation (BH), increasing the t11–18:1 and c9,t11–18:2 production. However, the adequate conditions of the C. ladanifer CTs used to optimize the production of t11–18:1 and c9,t11–18:2 is not yet known. Thus, we tested the effect of increasing the doses of C. ladanifer CT extract (0, 25, 50, 75 and 100 g/kg dry matter (DM)) on in vitro rumen BH. Five in vitro batch incubations replicates were conducted using an oil supplemented high-concentrate substrate, incubated for 24 h with 6 mL of buffered ruminal fluid. Volatile fatty acids (VFAs) and long chain fatty acids (FA) were analyzed at 0 h and 24 h, and BH of c9–18:1, c9, c12–18:2 and c9, c12, c15–18:3, and BH products yield were computed. Increasing doses of C. ladanifer CTs led to a moderate linear decrease (p < 0.001) of the VFA production (a reduction of 27% with the highest dose compared to control). The disappearance of c9–18:1 and c9,c12–18:2 as well as the production of t11–18:1 and c9, t11:18:2 was not affected by increasing doses of C. ladanifer CTs, and only the disappearance of c9, c12, c15–18:3 suffered a mild linear decrease (a reduction of 24% with the highest dose compared to control). Nevertheless, increasing the C. ladanifer CT dose led to a strong depression of microbial odd and branched fatty acids and of dimethyl acetals production (less than 65% with the highest dose compared to control), which indicates that microbial growth was more inhibited than fermentative and biohydrogenation activities, in a possible adaptative response of microbial population to stress induced to CTs and polyunsaturated fatty acids. The ability of C. ladanifer to modulate the ruminal BH was not verified in the current in vitro experimental conditions, emphasizing the inconsistent BH response to CTs and highlighting the need to continue seeking the optimal conditions for using CTs to improve the fatty acid profile of ruminant fat.
The Impact of Polyamine Precursors, Polyamines, and Steroid Hormones on Temporal Messenger RNA Abundance in Bovine Satellite Cells Induced to Differentiate
Authors: Reichhardt Caleb C. , Okamoto Lillian L. , Motsinger Laura A. , Griffin Brian P. , Murdoch Gordon K. , Thornton Kara J.
Published: March 2021

Emerging research suggests that hormones found in anabolic implants interact with polyamine biosynthesis. The objective of this study was to determine the effects of steroidal hormones, polyamines and polyamine precursors on bovine satellite cell (BSC) differentiation and polyamine biosynthesis temporally. Primary BSCs were induced to differentiate in 3% horse serum (CON) and treated with 10 nM trenbolone acetate (TBA), 10 nM estradiol (E2), 10 nM TBA and 10 nM E2, 10 mM methionine, 8 mM ornithine, 2 mM putrescine, 1.5 mM spermidine, or 0.5 mM spermine. Total mRNA was isolated 0, 2, 4, 8, 12, 24, and 48 h post-treatment. Abundance of mRNA for genes associated with induction of BSC differentiation: paired box transcription factor 7, myogenic factor 5, and myogenic differentiation factor 1 and genes in the polyamine biosynthesis pathway: ornithine decarboxylase and S-adenosylmethionine—were analyzed. Overall, steroidal hormones did not impact (p > 0.05) mRNA abundance of genes involved in BSC differentiation, but did alter (p = 0.04) abundance of genes involved in polyamine biosynthesis. Polyamine precursors influenced (p < 0.05) mRNA of genes involved in BSC differentiation. These results indicate that polyamine precursors and polyamines impact BSC differentiation and abundance of mRNA involved in polyamine biosynthesis, while steroidal hormones altered the mRNA involved in polyamine biosynthesis.
Effects of Lard and Vegetable Oils Supplementation Quality and Concentration on Laying Performance, Egg Quality and Liver Antioxidant Genes Expression in Hy-Line Brown
Authors: Zhang Junnan , Chen Jiajing , Yang Jing , Gong Sijia , Zheng Jiangxia , Xu Guiyun
Published: March 2021

This study examined the effects of various types, quality, and levels of dietary oils on laying performance and the expression patterns of antioxidant-related genes in Hy-line brown laying hens. A total of 720 40-week-old Hy-line brown laying hens were fed the same corn-soybean basal meals but containing 0.5 or 1.5% normal or oxidized soybean oil or lard, a total of 8 treatments. The results showed that laying rate (LR) and fatty acids of raw yolk were significantly correlated dietary type of oil (p < 0.05). With the increasing concentration of normal oil, it significantly increased LR and decreased feed conversion ratio (FCR, feed/egg) and albumen height of laying hens. The oxidized oil significant decreased the production performance of laying hens; and adding 1.5% of oxidized lard into feeds could destroy the integrity of yolk spheres of cooked yolk. mRNA expression of liver antioxidant-related genes increased when dietary oxidized oils were added into feeds. By comparing different qualities oil effect on antioxidant-related genes, the expression of Glutathione S-Transferase Theta 1 (GSTT1), Glutathione S-Transferase Alpha 3 (GSTA3), Glutathione S-Transferase Omega 2 (GSTO2), and Superoxide Dismutase 2 (SOD2) were increased when dietary oils were oxidized, in which change of the GSTO2 expression was the most with 1.5% of oxidized soybean oil. In conclusion, the ideal type of oil for Hy-line brown layer hens is soybean comparing with lard in a corn-soybean diet, avoiding using of oxidized oil.
Molecular Analysis of Polymyxin Resistance among Carbapenemase-Producing Klebsiella pneumoniae in Colombia
Authors: De La Cadena Elsa , Mojica María Fernanda , García-Betancur Juan Carlos , Appel Tobías Manuel , Porras Jessica , Pallares Christian José , Solano-Gutiérrez Juan Sebastián , Rojas Laura J. , Villegas María Virginia
Published: March 2021

Polymyxin resistance in Klebsiella pneumoniae has been attributed to mutations in mgrB, phoPQ, pmrAB, and crrAB and to the presence of mcr plasmid-mediated genes. Herein, we describe the molecular characteristics of 24 polymyxin- and carbapenem-resistant K. pneumoniae isolates recovered from six Colombian cities between 2009 and 2019. Minimum inhibitory concentrations (MICs) to polymyxin were confirmed by broth microdilution, and whole-genome sequencing was performed to determine sequence type, resistome, and mutations in the genes related to polymyxin resistance, as well the presence of mcr. The results showed high-level resistance to polymyxin (MICs ≥ 4 μg/mL). blaKPC-3 was present in the majority of isolates (17/24; 71%), followed by blaKPC-2 (6/24; 25%) and blaNDM-1 (1/24; 4%). Most isolates belonged to the CG258 (17/24; 71%) and presented amino acid substitutions in PmrB (22/24; 92%) and CrrB (15/24; 63%); mutations in mgrB occurred in only five isolates (21%). Additional mutations in pmrA, crrA, and phoPQ nor any of the mcr resistance genes were identified. In conclusion, we found clonal dissemination of polymyxin and carbapenem-resistant K. pneumoniae isolates in Colombia, mainly associated with CG258 and blaKPC-3. Surveillance of this multidrug-resistant clone is warranted due to the limited therapeutic options for the treatment of carbapenem-resistant K. pneumoniae infections.
Antimicrobial Effects of Minocycline, Tigecycline, Ciprofloxacin, and Levofloxacin against Elizabethkingia anophelis Using In Vitro Time-Kill Assays and In Vivo Zebrafish Animal Models
Authors: Lin Jiun-Nong , Lai Chung-Hsu , Huang Yi-Han , Yang Chih-Hui
Published: March 2021

Elizabethkingia anophelis is a multidrug-resistant pathogen. This study evaluated the antimicrobial activity of minocycline, tigecycline, ciprofloxacin, and levofloxacin using in vitro time-kill assays and in vivo zebrafish animal models. The E. anophelis strain ED853-49 was arbitrarily selected from a bacterial collection which was concomitantly susceptible to minocycline, tigecycline, ciprofloxacin, and levofloxacin. The antibacterial activities of single agents at 0.5–4 × minimum inhibitory concentration (MIC) and dual-agent combinations at 2 × MIC using time-kill assays were investigated. The therapeutic effects of antibiotics in E. anophelis-infected zebrafish were examined. Both minocycline and tigecycline demonstrated bacteriostatic effects but no bactericidal effect. Minocycline at concentrations ≥2 × MIC and tigecycline at concentrations ≥3 × MIC exhibited a long-standing inhibitory effect for 48 h. Bactericidal effects were observed at ciprofloxacin and levofloxacin concentrations of ≥3 × MIC within 24 h of initial inoculation. Rapid regrowth of E. anophelis occurred after the initial killing phase when ciprofloxacin was used, regardless of the concentration. Levofloxacin treatment at the concentration of ≥2 × MIC consistently resulted in the long-lasting and sustainable inhibition of bacterial growth for 48 h. The addition of minocycline or tigecycline weakened the killing effect of fluoroquinolones during the first 10 h. The minocycline-ciprofloxacin or minocycline–levofloxacin combinations achieved the lowest colony-forming unit counts at 48 h. Zebrafish treated with minocycline or a combination of minocycline and levofloxacin had the highest survival rate (70%). The results of these in vitro and in vivo studies suggest that the combination of minocycline and levofloxacin is the most effective therapy approach for E. anophelis infection.
Synthesis of Short-Chain-Fatty-Acid Resveratrol Esters and Their Antioxidant Properties
Authors: Tain You-Lin , Chang Sam K. C. , Liao Jin-Xian , Chen Yu-Wei , Huang Hung-Tse , Li Yu-Lun , Hou Chih-Yao
Published: March 2021

To expand the applications and enhance the stability and bioactivity of resveratrol (RE), and to simultaneously include the potential health benefits of short chain fatty acids (SCFA) esters of RE were prepared by Steglich reactions with acetic, propionic, and butyric acids, respectively. RE and the esterified RE-SCFA products (including RAE, RPE, and RBE) were analyzed using nuclear magnetic resonance (NMR), Fourier-transform infrared (FTIR) spectroscopy, thermogravimetric analysis (TGA), differential thermal analysis (DTA), and liquid chromatography–mass spectrometry (LC–MS). The FTIR and 13C NMR spectra of the esterified products included ester-characteristic peaks at 1751 cm−1 and 171 ppm, respectively. Moreover, the peaks in the range of 1700 to 1600 cm−1 in the FTIR spectra of the esterified products indicated that the esterification of RE-SCFA was successful. The TGA results revealed that the RE-SCFA esters decomposed at lower temperatures than RE. The peaks in the LC–MS profiles of the esterified products indicated the formation of mono- and diesters, and the calculated monoester synthesis rates ranged between 45.81 and 49.64%. The RE esters inhibited the Cu2+-induced low-density lipoprotein oxidation reaction, exhibited antioxidant activity in bulk oil, and effectively inhibited the hydroxyl radical-induced DNA scission. Moreover, the RE-SCFA esters had better hydrogen peroxide scavenging activity than RE. Our results are the first in the literature to successfully including short chain fatty acids in the esters of resveratrol, and the products could be used as a functional food ingredient in processed foods or can be used as dietary supplements to promote health.
IL17A Depletion Affects the Metabolism of Macrophages Treated with Gemcitabine
Authors: Roux Cecilia , Mucciolo Gianluca , Kopecka Joanna , Novelli Francesco , Riganti Chiara , Cappello Paola
Published: March 2021

Background: Interleukin (IL)17A is a member of the IL17 cytokine family, which is released by both immune and non-immune cells such as tumor and stromal cells into the tumor microenvironment. IL17 receptors are also widely expressed in different type of cells. Among all the members, IL17A is the most controversial in regulating tumor immunity. Here, we investigated how IL17A inhibition modulated macrophage differentiation and metabolism in the presence or absence of gemcitabine. Gemcitabine is the gold standard drug for treating pancreatic cancer and can increase macrophage antitumoral activities. Results: We observed some unique features of macrophages polarized in the absence of IL17A, in terms of RNA and protein expression of typical phenotypic markers, and we demonstrated that this paralleled specific changes in their metabolism and functions, such as the induction of an antitumor response. Interestingly, these features were almost maintained or enhanced when macrophages were treated with gemcitabine. We also demonstrated that the anti-IL17A antibody effectively reproduced features of macrophages derived from IL17A knock-out mice. Conclusion: Overall, we provide a proof-of-concept that combining an anti-IL17A antibody with gemcitabine may represent an effective strategy to modulate macrophages and enhance the anti-tumor response, especially in pancreatic cancer where gemcitabine is widely used.
Spicy Herb Extracts as a Potential Improver of the Antioxidant Properties and Inhibitor of Enzymatic Browning and Endogenous Microbiota Growth in Stored Mung Bean Sprouts
Authors: Sikora Małgorzata , Złotek Urszula , Kordowska-Wiater Monika , Świeca Michał
Published: March 2021

The quality and shelf life of sprouts can be improved by postharvest application of water herb extracts. The effect of water infusions of marjoram, oregano, basil, and thyme on the phenolic content, antioxidant potential, and the microbiological and consumer quality of stored mung bean sprouts was studied. Compared to the control, the treatments increased total phenolic content. The highest amounts were determined in sprouts soaked in the thyme extract (6.8 mg/g d.m.). The infusions also inhibited the activity of enzymes utilizing phenolics, and marjoram and oregano were found to be the most effective. The increase in the level of phenolics was reflected in enhanced antioxidant properties (ability to quench cation radical ABTS•+, reducing and chelating power). Both total phenolics and flavonoids, as well as antioxidant capacities, were highly bioaccessible in vitro. All the natural extracts effectively reduced the growth of total mesophilic bacteria, coliforms, and molds (they were more effective than ascorbic and kojic acids). The treatments did not exert a negative influence on the sensory properties or nutritional value of the sprouts, and even improved starch and protein digestibility. These results are very promising and may suggest a wider used of natural extracts as preservatives of minimally processed food.
An Active Irradiation System with Automatic Beam Positioning and Focusing for a Medical Cyclotron
Authors: Häffner Philipp Daniel , Belver-Aguilar Carolina , Casolaro Pierluigi , Dellepiane Gaia , Scampoli Paola , Braccini Saverio
Published: March 2021

A novel active focusing system was developed for enhancing the irradiation performance of the 18 MeV medical cyclotron in operation at the Bern University Hospital in view of the production of non-conventional medical radioisotopes using solid targets. In several cases, such as the production of 43Sc and 44Sc, the beam has to be kept stable within a very small target of about 5 mm diameter. For this purpose, we conceived and realized an apparatus based on a compact focusing and steering magnet system followed by a two-dimensional beam monitoring detector and a specific feedback software that drives the magnet to optimize the beam for a given irradiation set-up. We report on the design, realization and validation beam tests performed using the research beam transfer line of the Bern cyclotron. We demonstrated that the beam spot can be kept on target thanks to the fact that the system automatically reacts to perturbations. Compactness is one of the key features of this system, allowing its use in accelerator facilities with limited space, such as medical cyclotrons for radioisotope production.
Compressive Behavior of Pieces of Wood Reinforced with Fabrics Composed of Carbon Fiber and Basalt Fiber
Authors: de la Rosa Pilar , González María de las Nieves , Prieto María Isabel , Gómez Enrique
Published: March 2021

Given the importance of wood as a building material for hundreds of years, it is often necessary to increase the resilience of existing wood elements. Due to their notable adaptability and high mechanical capacities, materials composed of polymers that have been reinforced with fibers represent a good alternative to traditional reinforcement systems. This study looks at the compressive behavior of prismatic wild pinewood test pieces confined with three types of fabric—one of carbon fiber reinforced polymer (CFRP) and two of basalt fiber reinforced polymer (BFRP) featuring two very different grammages. The reinforced pieces attain at least 96% greater resistance than the unreinforced pieces (FC300), reaching as much as 133% more resistance in the case of the pieces reinforced with FB600; however, the ductility of the reinforced pieces is much lower than that of the unreinforced, by approximately one-sixth and one-quarter, respectively. The study also allowed us to conclude that there is no relationship between the mechanical capacity of the reinforcement and the ultimate strength of the test piece tested, but there is a strong relationship between the rigidity of the reinforcement and modulus of elasticity and the ultimate strain of the reinforced pieces.
Numerical Phase-Field Model Validation for Dissolution of Minerals
Authors: Yang Sha , Ukrainczyk Neven , Caggiano Antonio , Koenders Eddie
Published: March 2021

Modelling of a mineral dissolution front propagation is of interest in a wide range of scientific and engineering fields. The dissolution of minerals often involves complex physico-chemical processes at the solid–liquid interface (at nano-scale), which at the micro-to-meso-scale can be simplified to the problem of continuously moving boundaries. In this work, we studied the diffusion-controlled congruent dissolution of minerals from a meso-scale phase transition perspective. The dynamic evolution of the solid–liquid interface, during the dissolution process, is numerically simulated by employing the Finite Element Method (FEM) and using the phase–field (PF) approach, the latter implemented in the open-source Multiphysics Object Oriented Simulation Environment (MOOSE). The parameterization of the PF numerical approach is discussed in detail and validated against the experimental results for a congruent dissolution case of NaCl (taken from literature) as well as on analytical models for simple geometries. In addition, the effect of the shape of a dissolving mineral particle was analysed, thus demonstrating that the PF approach is suitable for simulating the mesoscopic morphological evolution of arbitrary geometries. Finally, the comparison of the PF method with experimental results demonstrated the importance of the dissolution rate mechanisms, which can be controlled by the interface reaction rate or by the diffusive transport mechanism.
Safety and Efficacy of a Modified Technique of Holmium Laser Enucleation of the Prostate (HoLEP) for Benign Prostatic Hyperplasia
Authors: Cochetti Giovanni , Del Zingaro Michele , Panciarola Mattia , Paladini Alessio , Guiggi Paolo , Ciarletti Sara , Nogara Andrea , Turco Morena , Marsico Matteo , Felici Graziano , Maiolino Giuseppe , Gaudio Gianluca , Mearini Ettore
Published: March 2021

Holmium laser enucleation of the prostate (HoLEP) is a valid alternative to transurethral resection of the prostate and open simple prostatectomy for the treatment of a larger prostate, demonstrating comparable efficacy and lower morbidity. One of the most bothersome symptoms after HoLEP is urinary incontinence (UI), which is present in almost 20% of patients, with a recovery rate of over 80% at 3 months. A relevant risk factor linked to UI is the damage of the external sphincter during the enucleation of adenoma tissue close to it. In our modified HoLEP technique named Cap HoLEP, we preserve the anterior prostate portion proximal to the external sphincter. This cap of adenoma could reduce mechanical stress and laser energy widespread on the sphincter, acting as a protective barrier. The aim of this study was to describe the Cap HoLEP technique and to evaluate its safety and efficacy by assessing peri-operative and functional outcomes. We enrolled all patients who consecutively underwent Cap HoLEP from December 2017 to October 2019 in our hospital. Baseline characteristics; the International Prostate Symptom Score; uroflow findings; intraoperative data, intraoperative, and postoperative complications; and UI were all assessed. The median operative time was 122 min with 138 kJ of laser energy delivered. Median ∆Hb was 0.8 gr/dL. Seven low-grade complications were recorded. At 1 month, 34.8% of patients presented UI, 16.7% urge incontinence, 13.6% stress incontinence, and 4.5% mixed incontinence. At 3 months, UI showed a significant improvement, decreasing to 12.1%. At 6 and 12 months, UI was 7.6% and 3%, respectively. Our modified HoLEP technique is safe and effective, allowing significant improvement in the postoperative UI rate.
Silver Nanoparticles Functionalized by Fluorescein Isothiocyanate or Rhodamine B Isothiocyanate: Fluorescent and Plasmonic Materials
Authors: Fratoddi Ilaria , Battocchio Chiara , Iucci Giovanna , Catone Daniele , Cartoni Antonella , Paladini Alessandra , O’Keeffe Patrick , Nappini Silvia , Cerra Sara , Venditti Iole
Published: March 2021

This paper presents the synthesis of silver nanoparticles (AgNPs) functionalized with fluorescent molecules, in particular with xanthene-based dyes, i.e., fluorescein isothiocyanate (FITC, λmax = 485 nm) and rhodamine B isothiocyanate (RITC, λmax = 555 nm). An in-depth characterization of the particle–dye systems, i.e., AgNPs–RITC and AgNPs–FITC, is presented to evaluate their chemical structure and optical properties due to the interaction between their plasmonic and absorption properties. UV–Vis spectroscopy and the dynamic light scattering (DLS) measurements confirmed the nanosize of the AgNPs–RITC and AgNPs–FITC. Synchrotron radiation X-ray photoelectron spectroscopy (SR-XPS) was used to study the chemical surface functionalization by structural characterization, confirming/examining the isothiocyanate–metal interaction. For AgNPs–RITC, in which the plasmonic and fluorescence peak are not superimposed, the transient dynamics of the dye fluorescence were also studied. Transient absorption measurements showed that by exciting the AgNPs–RITC sample at a wavelength corresponding to the AgNP plasmon resonance, it was possible to preferentially excite the RITC dye molecules attached to the surface of the NPs with respect to the free dye molecules in the solution. These results demonstrate how, by combining plasmonics and fluorescence, these AgNPs can be used as promising systems in biosensing and imaging applications.
Mathematical Modeling for the Growth of Salmonella spp. and Staphylococcus aureus in Cake at Fluctuating Temperatures
Authors: Lee Heeyoung , Park Jin Hwa , Park Yu Kyoung , Kim Hyun Jung
Published: March 2021

This study aimed to develop dynamic mathematical models to predict the growth of Salmonella spp. and Staphylococcus aureus in a cake under fluctuating temperatures. Among the nine different types of cakes frequently served during school meals, one type of cake was selected based on bacterial growth and water activity. Cocktails of Salmonella spp. and S. aureus were inoculated in the samples and stored at 4–35 °C for up to 336 h. The growth of Salmonella spp. and S. aureus was observed above 20 and 15 °C, respectively. The bacterial cell counts were fitted in the Baranyi model, and the maximum specific growth rate (μmax; log CFU/g/h) and lag phase duration (LPD; h) were analyzed using a polynomial model as a function of temperature (R2 = 0.968–0.988), and the performance of the developed models was appropriate. Furthermore, dynamic models were developed, and the predictions were acceptable in changing the temperature, indicating that the developed dynamic models can successfully predict the outcomes of Salmonella spp. and S. aureus in cake. These results provide useful information for assessing and managing microbial risk in foods by predicting the behavior of Salmonella spp. and S. aureus in cake, especially in changing temperature.
Virtual Reality Environment with Haptic Feedback Thimble for Post Spinal Cord Injury Upper-Limb Rehabilitation
Authors: Gutiérrez Álvaro , Farella Nicola , Gil-Agudo Ángel , de los Reyes Guzmán Ana
Published: March 2021

Cervical spinal cord injury is damage to the spinal cord that causes temporary or permanent changes in body functions below the site of the injury. In particular, the impairment of the upper limbs limits the patient’s autonomy in the execution of activities of daily living. This paper illustrates the use of a low-cost robot with a virtual reality platform for upper limb rehabilitation of cervical spinal cord injury patients. Vibration and pressure haptic feedback sensations are provided thanks to a custom-made thimble feedback device. The virtual reality platform consists of three different virtual rehabilitation games developed in Unity. They provide the user with the opportunity to interact with the virtual scene using free hands thanks to the data collected by a hand tracking system. During the therapy session, quantitative data about the motor performance are collected. Each virtual reality environment can be modified in settings according to the patients’ needs. A proof of concept was performed with both healthy subjects and spinal cord injured patients to evaluate the platform and its usability. The data saved during the sessions are analyzed to validate the importance of haptic feedback and stored both for patients and therapists to control the performance and the recovery process.
Experimental Study on Strengthening Effect Analysis of a Deteriorated Bridge Using External Prestressing Method
Authors: Kim Sang-Hyun , Park Jong-Sup , Jung Woo-Tai , Kim Tae-Kyun , Park Hee-Beom
Published: March 2021

The external prestressing method was developed to improve the load carrying capacity of concrete bridges damaged by ageing or other external environmental factors. Therefore, it is necessary to examine the performance of the structure before and after strengthening, and verification and confirmation of the actual strengthening effect should be preceded. However, for reasons such as securing deteriorated bridges and the absence of design data, the strengthening effect of an actual bridge is rarely analyzed. Hence, in this study, the strengthening effect was verified by conducting a set of material property tests and four-point loading tests before and after strengthening of an actual bridge, which had been utilized for over 45 years. As a result, it was determined that confirming the strengthening effect before cracking is challenging due to the nature of the external prestressing method, which has an insignificant effect on the stiffness; however, the effect of increasing the crack load of the external prestressing method was experimentally verified. The result of the study is expected to contribute to the determination of the load carrying capacity and required strengthening amount when strengthening existing prestressed concrete girder bridges.
The Functional Properties of Lactobacillus casei HY2782 Are Affected by the Fermentation Time
Authors: Jung Seung Hee , Hong Dong Ki , Bang So-Jung , Heo Keon , Sim Jae-Jung , Lee Jung-Lyoul
Published: March 2021

Maintaining probiotic effectiveness represents the most important task for the development of functional foods. Gastrointestinal stability and intestinal adhesion properties comprise one criterion for probiotic selection. Here, we investigated the benefits of milk fermented with Lactobacillus casei HY2782 at different fermentation times. The probiotic strain used was L. casei HY2782 and the reference strain was L. casei ATCC393 for comparisons. The samples were fermented for 7 days at 30 °C. We determined the pH, CFU/mL, survival rate during simulated gastrointestinal digestion, adhesion ability to HT-29 cells, and gene expression of tight-junction proteins known to regulate intestinal permeability in Caco-2 cells. L. casei HY2782 exhibited significantly higher survival rates in simulated gastrointestinal digestion during long-term fermentation than L. casei ATCC393. The adhesion ability to HT-29 cells was significantly increased with L. casei HY2782 (3.3% to 8.7%) after 7 days of fermentation; however, only a slight increase was observed for L. casei ATCC393 (3.1% to 4.7%). In addition, L. casei HY2782 can significantly increase the expression of genes encoding tight-junction proteins during long-term fermentation of milk. In conclusion, we confirmed that long-term fermentation could be a novel manufacturing process for fermented milk containing L. casei HY2782 and showed the beneficial effects.
The Influence of Bone Bruises on Bone Tunnel Enlargement Regarding ACL Rupture
Authors: Rajca Jolanta , Gzik Marek , Ficek Krzysztof
Published: March 2021

Purpose: The purpose was to determine the impact of the size of bone bruises (BBs) on bone tunnel enlargement (BTE) occurrence. Materials and methods: Twenty-four (24) patients who underwent anterior cruciate ligament reconstruction (ACLR) were included in this retrospective study. The measurements of BBs based on the initial MRI scan, bone tunnel size based on the control MRI scan, and the spatial determination of BB in relation to the bone tunnel location were evaluated. To analyze the relationship between BBs and BTE in homogeneous groups regarding the time from injury to ACLR (t(I-S)), the largest subgroup B (n = 15), in which t(I-S) was 31 to 60 days, was isolated for further investigation. Results: Based on subgroup B, a weak correlation (r = 0.33) existed between the BB volume and BTE size in the femur and tibia. Considering the relationship between the distance from the BB to the bone tunnel in the femur (f-l) and its enlargement (Δfd), there was a moderate and statistically significant (p < 0.05) negative correlation (r = −0.64). The correlation between those parameters was even stronger (r = −0.77) in subgroup B (time interval between injury and surgery ranged from 31 to 60 days). Conclusions: A retrospective analysis of MRI data in patients after ACL reconstruction surgery showed a relevant association between the distance from the BB to the bone tunnel and BTE in the femur. The relationship was not confirmed in the tibia.
On the Feasibility of Using Industrial Urea to Mitigate Thermal and Shrinkage Cracking in Concrete
Authors: Kim Hak-Young
Published: March 2021

Industrial urea is an organic compound that is widely used for various physiological processes and for producing nitrogen fertilizers. This study hypothesized that mixing urea with concrete would decrease the temperature and expansion of the cement microstructure abruptly due to the reaction with water and a cement hydration product. This study tested the hypothesis by experiments. The performance of concrete was evaluated after setting the mixing ratio of industrial urea was to 0%, 5%, 10%, and 15%. The results showed that temperature decreased drastically immediately after mixing and the decrease in temperature increased with a higher mixing ratio. Consequently, condensation was delayed, and heat of hydration decreased. It was found that when considering workability and compressive strength development performance, the proper mixing ratio of urea was around 10%. This study proposed the mixing design technique of concrete mixed with urea. Additionally, this study reported better carbonation resistance and mitigated shrinkage strain of concrete mixed with urea compared to existing low-heat mixing ratios.
Locating Image Sources from Multiple Spatial Room Impulse Responses
Authors: Puomio Otto , Meyer-Kahlen Nils , Lokki Tapio
Published: March 2021

Measured spatial room impulse responses have been used to compare acoustic spaces. One way to analyze and render such responses is to apply parametric methods, yet those methods have been bound to single measurement locations. This paper introduces a method that locates image sources from spatial room impulse responses measured at multiple source and receiver positions. The method aligns the measurements to a common coordinate frame and groups stable direction-of-arrival estimates to find image source positions. The performance of the method is validated with three case studies—one small room and two concert halls. The studies show that the method is able to locate the most prominent image sources even in complex spaces, providing new insights into available Spatial Room Impulse Response (SRIR) data and a starting point for six degrees of freedom (6DoF) acoustic rendering.
Seasonal Estimates and Uncertainties of Snow Accumulation from CloudSat Precipitation Retrievals
Authors: Duffy George , King Fraser , Bennartz Ralf , Fletcher Christopher G.
Published: March 2021

CloudSat is often the only measurement of snowfall rate available at high latitudes, making it a valuable tool for understanding snow climatology. The capability of CloudSat to provide information on seasonal and subseasonal time scales, however, has yet to be explored. In this study, we use subsampled reanalysis estimates to predict the uncertainties of CloudSat snow water equivalent (SWE) accumulation measurements at various space and time resolutions. An idealized/simulated subsampling model predicts that CloudSat may provide seasonal SWE estimates with median percent errors below 50% at spatial scales as small as 2° × 2°. By converting these predictions to percent differences, we can evaluate CloudSat snowfall accumulations against a blend of gridded SWE measurements during frozen time periods. Our predictions are in good agreement with results. The 25th, 50th, and 75th percentiles of the percent differences between the two measurements all match predicted values within eight percentage points. We interpret these results to suggest that CloudSat snowfall estimates are in sufficient agreement with other, thoroughly vetted, gridded SWE products. This implies that CloudSat may provide useful estimates of snow accumulation over remote regions within seasonal time scales.
Prevalence of Prostate Cancer at Different Clinical Stages in Italy: Estimated Burden of Disease Based on a Modelling Study
Authors: Spandonaro Federico , D’Angela Daniela , Polistena Barbara , Bruzzi Paolo , Iacovelli Roberto , Luccarini Irene , Stagni Paola , Brigido Alessia
Published: March 2021

Understanding the distribution of prostate cancer (PC) at various clinical stages of disease is of utmost importance to quantify the cancer care needs of patients and to adequately plan health services. The aim of this analysis is thus to provide a model-based estimation of the number of prevalent PC patients at different clinical stages in the Italian setting. A simulation model of patient transitions was constructed on a yearly basis using data obtained through a literature review on the incidence, prevalence, progression and mortality of PC, with specific focus on disease stage. A total of 462,570 prevalent PC patients were estimated at 1 January 2019. According to the model, 94.8% of them had non-metastatic PC and 5.2% had metastatic disease. Among the non-metastatic patients, most had T1/T2 PC (85.6%), followed by T3/T4 (10.9%) and T0/Tx PC (3.6%). About 20% of the T3/T4 patients had biochemically recurrent PC. Among the metastatic PC patients, 66.1% had castration-resistant PC and 33.9% had hormone-sensitive PC. This study provided original information on the distribution of PC according to different clinical stages that may be useful to define strategies, understand the PC disease pathway, estimate treatment-related needs and, possibly, plan targeted interventions for public health management of prostate cancer in Italy.
Bio-Inspired Design of a Porous Resorbable Scaffold for Bone Reconstruction: A Preliminary Study
Authors: Scerrato Daria , Bersani Alberto Maria , Giorgio Ivan
Published: March 2021

The study and imitation of the biological and mechanical systems present in nature and living beings always have been sources of inspiration for improving existent technologies and establishing new ones. Pursuing this line of thought, we consider an artificial graft typical in the bone reconstruction surgery with the same microstructure of the bone living tissue and examine the interaction between these two phases, namely bone and the graft material. Specifically, a visco-poroelastic second gradient model is adopted for the bone-graft composite system to describe it at a macroscopic level of observation. The second gradient formulation is employed to consider possibly size effects and as a macroscopic source of interstitial fluid flow, which is usually regarded as a key factor in bone remodeling. With the help of the proposed formulation and via a simple example, we show that the model can be used as a graft design tool. As a matter of fact, an optimization of the characteristics of the implant can be carried out by numerical investigations. In this paper, we observe that the size of the graft considerably influences the interaction between bone tissue and artificial bio-resorbable material and the possibility that the bone tissue might substitute more or less partially the foreign graft for better bone healing.
Is Lateropulsion Really Related with a Specific Lesion of the Brain?
Authors: Lee Kyoung Bo , Yoo Sang Won , Ji Eun Kyu , Hwang Woo Seop , Yoo Yeun Jie , Yoon Mi-Jeong , Hong Bo Young , Lim Seong Hoon
Published: March 2021

Lateropulsion (pusher syndrome) is an important barrier to standing and gait after stroke. Although several studies have attempted to elucidate the relationship between brain lesions and lateropulsion, the effects of specific brain lesions on the development of lateropulsion remain unclear. Thus, the present study investigated the effects of stroke lesion location and size on lateropulsion in right hemisphere stroke patients. The present retrospective cross-sectional observational study assessed 50 right hemisphere stroke patients. Lateropulsion was diagnosed and evaluated using the Scale for Contraversive Pushing (SCP). Voxel-based lesion symptom mapping (VLSM) analysis with 3T-MRI was used to identify the culprit lesion for SCP. We also performed VLSM controlling for lesion volume as a nuisance covariate, in a multivariate model that also controlled for other factors contributing to pusher behavior. VLSM, combined with statistical non-parametric mapping (SnPM), identified the specific region with SCP. Lesion size was associated with lateropulsion. The precentral gyrus, postcentral gyrus, inferior frontal gyrus, insula and subgyral parietal lobe of the right hemisphere seemed to be associated with the lateropulsion; however, after adjusting for lesion volume as a nuisance covariate, no lesion areas were associated with the SCP scores. The size of the right hemisphere lesion was the only factor most strongly associated with lateropulsion in patients with stroke. These results may be useful for planning rehabilitation strategies of restoring vertical posture and understanding the pathophysiology of lateropulsion in stroke patients.