bluuebunny/crossref_metadata_2025 huggingface dataset:
PENGETAHUAN KESEHATAN REPRODUKSI REMAJA DALAM MENCEGAH PENYIMPANGAN SEKSUAL
Authors: Yarza Husnin Nahry , Maesaroh  , Kartikawati Eka
Published: December 2019

Abstract

This community service aims to direct the community to a healthy lifestyle related to reproductive health. The subjects of service are high school adolescents who are vulnerable to the negative influence of social and environmental media. Reproductive health is very important for both men and women. Reproductive health is defined as a whole physical, mental and social well-being not free from disease or disability in all matters relating to the reproductive system, its functions and processes. This service at the high school level is very necessary to be carried out by using location search methods, approaches to the school and providing workshops to students. With this service can provide students with knowledge and insight into reproductive health and sexual deviations that they must avoid. Adolescent reproductive health is an inseparable part of mental health, mental health and social health. Teenagers must understand and know their sexual reproductive health so as not to produce sexual deviations that will harm themselves and their families.

Abstrak

Pengabdian  masyarakat ini bertujuan untuk mengarahkan masyarakat pada pola hidup sehat terkait dengan kesehatan reproduksi. Subjek pengabdian yaitu remaja tingkat SMA yang rentan terhadap pengaruh negatif media sosial dan lingkungan. Kesehatan reproduksi hal yang sangat penting untuk pria maupun wanita. Kesehatan reproduksi didefenisikan sebagai suatu kesejahteraan fisik, mental dan sosial secara utuh tidak bebas dari penyakit atau kecacatan dalam semua hal yang berkaitan dengan sistem reproduksi, fungsi dan prosesnya. Pengabdian di tingkat sekolah menengah atas ini sangat perlu untuk dilak dengan menggunakan metode pencarian lokasi, pendekatan kepada pihak sekolah dan pemberian workshop kepada peserta didik.. Dengan adanya pengabdian ini dapat memberikan pengetahuan dan wawasan siswa dalam kesehatan reproduksi dan penyimpangan seksual yang harus mereka hindari. Kesehatan reproduksi remaja merupakan bagian yang tidak dapat dipisahkan yakni kesehatan psikis, mental dan kesehatan social. Remaja harus memahami dan mengetahui kesehatan reproduksi seksual mereka agar tidak menghasilkan penyimpangan-penyimpangan seksual yang akan merugikan diri sendiri dan keluarga.
Design Features when Using an Effective Microturbine as a Range Extending Engine
Authors: Nadareishvili G. , Kostyukov A. , Karpukhin K.
Published: December 2019

The rapid development of electric vehicles stimulates the development of structures related to their operation. Including the use of the range extending engine for electric vehicles. The use of gas turbine engines for vehicles has always been of interest. The microturbine in the range extending engine is relevant today. However, the possibility of using a microturbine as part of a range extending engine is possible under several conditions. Microturbine should be successfully mated with a high-speed generator, and the whole structure should have a high efficiency and be economically advantageous. To create such a design, it is necessary to develop a simple microturbine with a design, equipped with a highly efficient heat exchanger, which allows to obtain high fuel and operational efficiency. Microturbine should have low temperatures on the turbine wheel to maintain high environmental parameters. The use of composite materials is necessary. The results of the development and technical characteristics of a single shaft microturbine of this class for use with a high-boring generator are presented, and its applicability as a range extending engine is justified. The main problems solved in the design of microturbines are shown: issues of thermal conditions, optimization of blade machines, flow in gas-air pipes. Power plants, based on the microturbine and high-speed generator, can be widely adopted on the basis of their simple and high operating characteristics, including the range extending engine as a range extending engine.
Electric Propulsion Systems Design Supported by Multi-Objective Optimization Strategies
Authors: Hirz M. , Hofstetter M. , Lechleitner D.
Published: December 2019

Electric drive systems consisting of battery, inverter, electric motor and gearbox are applied in hybridor purely electric vehicles. The layout process of such propulsion systems is performed on system level under consideration of various component properties and their interfering characteristics. In addition, different boundary conditions are taken under account, e. g. performance, efficiency, packaging, costs. In this way, the development process of the power train involves a broad range of influencing parameters and periphery conditions and thus represents a multi-dimensional optimization problem. Stateof-the-art development processes of mechatronic systems are usually executed according to the V-model, which represents a fundamental basis for handling the complex interactions of the different disciplines involved. In addition, stage-gate processes and spiral models are applied to deal with the high level of complexity during conception, design and testing. Involving a large number of technical and economic factors, these sequential, recursive processes may lead to suboptimal solutions since the system design processes do not sufficiently consider the complex relations between the different, partially conflicting domains. In this context, the present publication introduces an integrated multi-objective optimization strategy for the effective conception of electric propulsion systems, which involves a holistic consideration of all components and requirements in a multi-objective manner. The system design synthesis is based on component-specific Pareto-optimal designs to handle performance, efficiency, package and costs for given system requirements. The results are displayed as Pareto-fronts of electric power train system designs variants, from which decision makers are able to choose the best suitable solution. In this way, the presented system design approach for the development of electrically driven axles enables a multi-objective optimization considering efficiency, performance, costs and package. It is capable to reduce development time and to improve overall system quality at the same time.
Validation of an Assistance System for Merging Maneuvers in Highways in Real Driving Conditions
Authors: Sanchez-Mateo S. , Perez-Moreno E. , Jimenez F. , Serradilla F. , Cruz Ruiz A. , De la Fuente Tamayo S.
Published: December 2019

In the latest study conducted by the National Highway Traffic Safety Administration in 2018, it was published that human error is still considered the major factor in traffic accidents, 94 %, compared with other causes such as vehicles, environment and unknown critical reasons. Some driving scenarios are especially complex, such as highways merging lanes, where the driver obtains information from the environment while making decisions on how to proceed to perform the maneuver smoothly and safely. Ignorance of the intentions of the drivers around him leads to risky situations between them caused by misunderstandings or erroneous assumptions or perceptions. For this reason, Advanced Driver Assistance Systems could provide information to obtain safer maneuvers in these critical environments. In previous works, the behavior of the driver by means of a visual tracking system while merging in a highway was studied, observing a cognitive load in those instants due to the high attentional load that the maneuver requires. For this reason, a driver assistance system for merging situations is proposed. This system uses V2V communications technology and suggests to the driver how to modify his speed in order to perform the merging manoeuver in a safe way considering the available gap and the relative speeds between vehicles. The paper presents the results of the validation of this system for assisting in the merging maneuver. For this purpose, the interface previously designed and validated in terms of usability, has been integrated into an application for a mobile device, located inside the vehicle and tests has been carried out in real driving conditions.
Contexto y resultados del desarrollo infantil temprano en niños y niñas de 12 a 59 meses en México
Authors: De Castro Filipa , Vázquez-Salas Ruth Argelia , Villalobos Aremis , Rubio-Codina Marta , Prado Eva , Sánchez-Ferrer José Carlos , Romero Martín , Shamah-Levy Teresa
Published: December 2019

Objetivo. Evaluar el desarrollo infantil temprano (DIT) y sus determinantes en niños/as de 12 a 59 meses residentes en localidades de menos de 100 000 habitantes. Material y métodos. La Encuesta Nacional de Salud y Nutrición en localidades con menos de 100 000 habitantes (Ensanut 100k) evaluó el nivel de lenguaje, acceso a servicios de atención al DIT e indicadores de calidad del contexto de desarrollo. Se estiman prevalencias de indicadores y puntajes estandarizados de lenguaje según variables de interés. Resultados. 20.7% de los niños/as asistió a ocho consultas del niño sano en su primer año, 13.0% recibió evaluación de DIT, 75.0% recibe apoyo al aprendizaje, 23.4% cuenta con libros y 57.7% sufre disciplina violenta. Mejores niveles de lenguaje se asocian con las capacidades económicas, escolaridad materna, asistencia a preescolar, apoyo al aprendizaje y acceso a libros. Los niños/ as expuestos a más factores protectores presentan nivel de lenguaje 1.5 DE mayor que en niños/as con más factores de riesgo. Conclusión. Se requiere aumentar la cobertura de atención al DIT y mejorar las oportunidades de desarrollo en hogares.
Caracterización de la “cascada de atención” en servicios públicos en México en localidades de menos de 100 000 habitantes
Authors: Colchero M Arantxa , Gómez Rouselinne , Bautista-Arredondo Sergio
Published: December 2019

Objetivo. Caracterizar la demanda de servicios de atención en la población que vive en localidades de menos de 100 000 habitantes. Material y métodos. Se utilizaron los datos de la Encuesta Nacional de Salud y Nutrición (Ensanut) 2018. Se caracterizó la cascada de atención y se estimaron modelos probit para analizar factores asociados con la probabilidad de recibir atención. Resultados. En la población que reportó haber tenido un problema de salud, sólo 32% se atendió en una institución pública. Se encontró que ser mujer, tener más de 50 años, tener seguridad social, percibir que el problema de salud es grave y pertenecer al nivel socioeconómico alto —dentro de este grupo de población— se asocia con mayor probabilidad de recibir atención. Conclusiones. Los hallaz­gos de este estudio abordan algunos retos y oportunidades para otorgar servicios públicos a la población que vive en áreas más marginadas.
Does Polymer's Viscoelasticity Influence Heavy-Oil Sweep Efficiency and Injectivity at 1 ft/D?
Authors: Azad Madhar S. , Trivedi Japan J.
Published: December 2019

For heavy-oil-recovery applications, mobility control is more important than interfacial-tension reduction, and therefore importance should be given to the recovery of remaining mobile oil by enhanced sweep efficiency. Although the relative roles of polymer viscosity and elasticity in capillary-trapped residual light-oil recovery have been studied extensively, their roles in sweeping mobile viscous oil have not been explored. Injectivity is vital for heavy-oil-recovery applications, and polymer selection is performed solely using criteria that is based on shear rheology. In this paper, the influence of viscous (shear) resistance and elastic (extensional) resistance of viscoelastic polymer on mobile-heavy-oil recovery and injectivity is investigated through the combination of bulk shear/extensional rheology and single-phase and multiphase coreflood experiments at a typical reservoir-flooding rate of 1 ft/D.

Two polymer solutions with different concentrations and salinities are selected such that a polymer with low molecular weight (MW) [hydrolyzed polyacrylamide (HPAM) 3130] provides higher shear resistance than a high-MW polymer (HPAM 3630). Extensional characterization of these two polymer solutions performed using a capillary breakup extensional rheometer revealed that HPAM 3630 provided higher extensional viscosity than HPAM 3130. The results show that the behaviors of polymers in extension and shear are completely different. Two multiphase and two single-phase experiments are conducted at low flux rate to investigate the roles of extensional viscosity on mobile-heavy-oil recovery and high flux rates on injectivity. After 1 pore volume (PV) of polymer injections, higher-concentration and lower-MW HPAM 3130 contributes to approximately 17% higher incremental recovery factor vs. lower-concentration and higher-MW HPAM 3630. The core-scale pressure drop generated by HPAM 3130 is more than twice the pressure drop generated by HPAM 3630. Under low-flux-rate conditions at the core scale, shear forces dominate, and displacing fluid with higher shear viscosity contributes to better sweep. HPAM 3630 exhibits a shear-thickening phenomenon and possesses the apparent viscosity of approximately 90 cp at the flux rate of approximately 90 ft/D. In contrast, HPAM 3130 continued showing shear thinning and has the apparent viscosity of approximately 70 cp at approximately 90 ft/D. This signifies the role of extension rheology on the injectivity at higher flux rates.

Results revealed that while the extensional rheological role toward sweeping the mobile heavy-oil recovery at low flux is lesser compared with the shear role, its negative role on the polymer injectivity is very significant. Polymer-selection criteria for heavy-oil-recovery applications should incorporate extensional rheological parameters.
Preventing acute kidney injury in high-risk patients by temporarily discontinuing medication – an observational study in general practice
Authors: Faber Suzanne J , Scherpbier Nynke , Peters Hans , Uijen Annemarie
Published: December 2019

Background Elderly, patients with chronic kidney disease (CKD) and patients with heart failure who continue using renin-angiotensin-aldosterone-system (RAAS) inhibitors, diuretics, or non-steroidal-anti-inflammatory drugs (NSAIDs) during times of fluid loss have a high risk of developing complications like acute kidney injury (AKI). The aim of this study was to assess how often advice to discontinue high-risk medication was offered to high-risk patients consulting the general practitioner (GP) with increased fluid loss. Furthermore, we assessed the number and nature of the complications that occurred after GP consultation. Methods We performed a cross-sectional study with patients from seven Dutch general practices participating in the Family Medicine Network between 1-6-2013 and 1-7-2018. We included patients who used RAAS-inhibitors, diuretics, or NSAIDs, and had at least one of the following risk factors: age ≥70 years, CKD, or heart failure. From this population, we selected patients with a ‘dehydration-risk’ episode (vomiting, diarrhoea, fever, chills, or gastrointestinal infection). We manually checked their electronic patient files and assessed the percentage of episodes in which advice to discontinue the high-risk medication was offered and whether a complication occurred in three months after the ‘dehydration-risk’ episode.Results We included 3607 high-risk patients from a total of 44.675 patients (8.1%). We found that patients were advised to discontinue the high-risk medication in 38 (4.6%) of 816 ‘dehydration-risk’ episodes. In 59 of 816 episodes (7.1%) complications (mainly AKI) occurred. Conclusions Dutch GPs do not frequently advise high-risk patients to discontinue high-risk medication during ‘dehydration-risk’ episodes. Complications occur frequently. Timely discontinuation of high-risk medication needs attention.
Challenges with Scale-up of GeneXpert MTB/RIF® inUganda: a Health Systems Perspective
Authors: Nalugwa Talemwa , Shete Priya B. , Nantale Mariam , Farr Katherine , Ojok Christopher , Ochom Emma , Mugabe Frank , Joloba Moses , Dowdy David W. , Moore David A.J. , Davis J. Lucian , Cattamanchi Adithya , Katamba Achilles
Published: December 2019

Background: Many high burden countries are scaling-up GeneXpertÒMTB/RIF (Xpert) testing for tuberculosis (TB) using a hub-and-spoke model. However, the effect of scale up on reducing TB has been limited. We sought to characterize variation in implementation of referral-based Xpert TB testing across Uganda, and to identify healthsystem factors that may enhance or prevent high-qualityimplementation of Xpert testing services. Methods: We conducted a cross-sectional study triangulatingquantitative and qualitative data sources at 23 community health centers linked to one of 15 Xpert testing sites between November 2016 and May 2017 to assess health systems infrastructure for hub-and-spoke Xpert testing. Data sources included a standardized site assessment survey, routine TB notification data, and field notes from site visits. Results: Challenges with Xpert implementation occurred at every step of the diagnostic evaluation process, leading to low overall uptake of testing. Of 2,192 patients eligible for TB testing, only 574 (26%) who initiated testing were referred for Xpert testing. Of those, 54 (9.4%)were Xpert confirmed positive just under half initiated treatment within 14 days (n=25, 46%). Gaps in required infrastructure at 23 community health centers to support the hub-and-spoke system included lack of refrigeration (n=14, X%) for sputum testing and lack of telephone/mobile communication (n=21, 91%). Motorcycle riders responsible for transporting sputum to Xpert sites operated variable with trips once a week, 2x/week or 3x/week at 10 (43%), 9 (39%) and 4 (17%) health centers, respectively. Staff recorded Xpert results in the TB laboratory register at only one health center and called patients with positive results at only 2 health centers. Of the 15 Xpert testing sites, 5 (33%) had at least one non-functioning module. The median number of tests per day was 3.57 (IQR 2.06-4.54), and 10 (67%) sites had error/invalid rates >5%. Conclusions: Although Xpert devices are now widely distributed throughout Uganda, health system factors across the continuum from test referral to results reporting and treatment initiation preclude effective implementation of Xpert testing for patients presenting to peripheral health centers. Support for scale up of innovative technologies should include support for communication, coordination and health systems integration.
Comparison of open liver resection and RFA for the treatment of solitary 3-5 cm hepatocellular carcinoma: a retrospective study
Authors: Lei Jianyong , Yan LN , Li DJ , Wang WT
Published: December 2019

Background: The goal of this study was to compare the postoperative results of liver resection and radiofrequency ablation (RFA) for the treatment of small hepatocellular carcinoma (HCC) (3-5 cm). Patients and methods: We retrospectively collected 122 cases of small solitary HCC treated at our center from Jan 2011 to Dec 2015, with diameters in the range of 3-5 cm. According to the treatment program received at our center, the patients were divided into liver resection (72 patients) and RFA (50 patients) groups. Result: In comparison with the RFA group, the resection group had a longer operative time, greater intraoperative blood loss (P<0.01), more hepatic inflow occlusion, and a longer postoperative hospital stay (P<0.01). The 1-, 3-, and 5-year expected overall survival rates and tumor-free survival rates were comparable between the two groups. Cox regression analysis showed that neither resection nor RFA was a significant risk factor for overall or tumor-free survival in HCC. Conclusions: For solitary HCC of 3-5 cm in diameter, RFA can achieve better in-hospital clinical results and similar long-term outcomes than resection and can be considered for wide application, especially for central-location cases.
Transcriptome profiling of laser-captured germ cells and functional characterization of zbtb40 during 17alpha-methyltestosterone-induced spermatogenesis in orange-spotted grouper (Epinephelus coioides)
Authors: Wu Xi , Yang Yang , Zhong Chaoyue , Guo Yin , Li Shuisheng , Lin Haoran , Liu Xiaochun
Published: December 2019

Background: Spermatogenesis is an intricate process regulated by a finely organized network. The orange-spotted grouper (Epinephelus coioides) is a protogynous hermaphroditic fish, but the regulatory mechanism of its spermatogenesis is not well-understood. In the present study, transcriptome sequencing of the male germ cells isolated from orange-spotted grouper was performed to explore the molecular mechanism underlying spermatogenesis. Results: In this study, the orange-spotted grouper was induced to change sex from female to male by 17alpha-methyltestosterone (MT) implantation. During the spermatogenesis, male germ cells (spermatogonia, spermatocytes, spermatids, and spermatozoa) were isolated by laser capture microdissection. Transcriptomic analysis for the isolated cells was performed. A total of 244,984,338 clean reads were generated from four cDNA libraries. Real-time PCR results of 13 genes related to sex differentiation and hormone metabolism indicated that transcriptome data are reliable. RNA-seq data showed that the female-related genes and genes involved in hormone metabolism were highly expressed in spermatogonia and spermatozoa, suggesting that these genes participate in the spermatogenesis. Interestingly, the expression of zbtb family genes showed significantly changes in the RNA-seq data, and their expression patterns were further examined during spermatogenesis. The analysis of cellular localization of Eczbtb40 and the co-localization of Eczbtb40 and Eccyp17a1 in different gonadal stages suggested that Eczbtb40 might interact with Eccyp17a1 during spermatogenesis. Conclusions: For the first time, our study investigated the transcriptome of the male germ cells from orange-spotted grouper, and identified functional genes, GO terms, and KEGG pathways involved in spermatogenesis. Furthermore, Eczbtb40 was first characterized and predicted the role during spermatogenesis. These data will contribute to future studies on the molecular mechanism of spermatogenesis in teleosts.
Comparative transcriptomics of stem bark reveals genes associated with bast fiber development in Boehmeria nivea L. Gaud (ramie)
Authors: Xie Jiyong , Li Jiaqi , Jie Yucheng , Xie Deyu , Yang Di , Shi Huazhong , Zhong Yingli
Published: December 2019

Background: Boehmeria nivea L. Gaud (Ramie) produces one of the longest natural fibers in nature. The bark of ramie mainly comprises of the phloem tissue of stem and is the raw material for fiber. Therefore, identifying the molecular regulation of phloem development is important for understanding of bast fiber biosynthesis and improvement of fiber quality in ramie.
Results: In this study, we collected top bud (TB), bark from internode elongating region (ER) and bark from internode fully elongated region (FER) from the ramie variety Zhongzhu No. 1. Histological study indicated that these samples contain phloem tissues at different developmental and maturation stages, with a higher degree of maturation of phloem tissue in FER. RNA sequencing (RNA-seq) was performed to identify differentially expressed genes (DEGs) in these three samples. The de novo transcriptome was assembled and unigenes were identified. The DEGs among TB, ER and FER were analyzed, and Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) studies revealed clear differences in gene expression between ER and FER. Some unigenes for secondary cell wall biosynthesis were up-regulated in both ER and FER, while unigenes for some cell wall components or cell wall modifications showed differential expression between ER and FER. In addition, ethylene activating pathway and gibberellins biosynthesis showed distinct regulation and coincided with the developmental stages of the samples.
Conclusions: Both morphological study and gene expression analysis supported a burst of phloem and vascular developmental processes during the fiber maturation in the ramie stem, and the phytohormon ethylene and gibberellin are likely to be involved in this process. Our findings provide novel insights into the phloem development and fiber maturation in ramie, which could be useful for fiber improvement in ramie and other fiber crops.
MEG3 promotes proliferation and inhibits apoptosis in osteoarthritis chondrocytes by miR-361-5p/FOXO1 axis
Authors: Wang Anying , Hu Naixia , Zhang Yefeng , Chen Yuanzhen , Su Changhui , Lv Yao , Shen Yong
Published: December 2019

Background: This study aimed to investigate the role of long non-coding RNA (lncRNA) maternally expressed 3 (MEG3) and related molecular mechanisms in osteoarthritis (OA). Methods: Patients with OA and patients undergoing thigh amputation were involved in OA group and normal group, respectively. Cartilage tissues of all patients were isolated and cultured. Based on different transfection, MEG3 cells were grouped into Blank, pcDNA3.1-NC, pcDNA3.1-MEG3, si-NC, si-MEG3, pcDNA3.1-NC + mimics NC, pcDNA3.1-MEG3 + mimics NC, pcDNA3.1-NC + miR-361-5p mimics and pcDNA3.1-MEG3 + miR-361-5p mimics group. The RT-qPCR was used to detect the expression of MEG3, miR-361-5p and FOXO1. Moreover, western blot, luciferase reporter assay, RIP, CCK-8 and flow cytometry analysis were performed to reveal the morphology, proliferation and apoptosis in cartilage cells. Finally, the histological analysis and immunostaining were performed on OA rat model. Results: The expression of MEG3 and FOXO1 in OA was significantly decreased while miR-361-5p was increased compared with the normal group. MEG3 might serve as a ceRNA of miR-361-5p in OA chondrocytes. Moreover, the western blot and CCK-8 assay showed that MEG3, targeted miR-361-5p/FOXO1, might elevate cell proliferation and impair cell apoptosis. Finally, rat model analysis showed that MEG3 suppressed the cartilage matrix degradation. Conclusion: Taken together, MEG3 can contribute to cell proliferation, inhibit cell apoptosis and extracellular matrix (ECM) degradation via miR-361-5p/FOXO1 axis in OA chondrocytes.
In vivo model to study the impact of genetic variation on clinical outcome of mastitis in dairy uniparous cows
Authors: Rohmeier Laura , Petzl Wolfram , Koy Mirja , Eickhoff Tordis , Hülsebusch Alina , Jander Sebastian , Macias Laura , Heimes Annika , Engelmann Susanne , Hoedemaker Martina , Seyfert Hans-Martin , Kühn Christa , Schuberth Hans-Joachim , Zerbe Holm , Meyerholz Marie Margarete
Published: December 2019

Title: In vivo model to study the impact of genetic variation on clinical outcome of mastitis in dairy uniparous cows

Background: In dairy herds, mastitis causes detrimental economic losses. Genetic selection offers a sustainable tool to select animals with reduced susceptibility towards postpartum diseases. Studying underlying mechanisms is important to assess the physiological processes that cause differences between selected haplotypes. Therefore, the objective of this study was to establish an in vivo infection model to study the impact of selecting for alternative paternal haplotypes in a particular genomic region on cattle chromosome 18 for mastitis susceptibility under defined conditions in dairy uniparous cows.

Results: At the start of pathogen challenge, no significant differences between the favorable (Q) and unfavorable (q) haplotypes were detected. Intramammary infection (IMI) with Staphylococcus aureus 1027 (S. aureus, n = 24, 96 h) or Escherichia coli 1303 (E. coli, n = 12, 24 h) was successfully induced in all uniparous cows. This finding was confirmed by clinical signs of mastitis and repeated recovery of the respective pathogen from milk samples of challenged quarters in each animal. After S. aureus challenge, Q-uniparous cows showed lower somatic cell counts 24 h and 36 h after challenge (P < 0.05), lower bacterial shedding in milk 12 h after challenge (P < 0.01) and a minor decrease in total milk yield 12 h and 24 h after challenge (P < 0.01) compared to q-uniparous cows.

Conclusion: An in vivo infection model to study the impact of genetic selection for mastitis susceptibility under defined conditions in dairy uniparous cows was successfully established and revealed significant differences between the two genetically selected haplotype groups. This result might explain their differences in susceptibility towards IMI. These clinical findings form the basis for further in-depth molecular analysis to clarify the underlying genetic mechanisms for mastitis resistance.
MicroRNA Expression Profiles Analysis in Sperm Reveals hsa-mir-191 is an Auspicious Omen of in Vitro Fertilization
Authors: Xu Hua , Wang Xin , Wang Zhikai , Li Jianhui , Xu Zhiming , Miao Maohua , Chen Guowu , Lei Xiangdong , Wu Jun , Shi Huijuan , Wang Ke , Zhang Tiancheng , Sun Xiaoxi
Published: December 2019

Background: MicroRNAs (miRNAs) are a class of noncoding small RNAs that play important roles in many physiological processes by regulating gene expression. Previous studies have shown that the expression levels of total miRNAs increase during mouse embryonic development, and some miRNAs control the regulatory network in development progression. However, few studies have focused on the effects of miRNAs on early human embryonic development. The relationship between miRNAs and early human embryogenesis is still unknown. Results:In this study, RNA-seq data collected from sperm samples from 102 patients with a normal sperm index but treated with assisted reproductive technology (ART) were analyzed for the relationships between differentially expressed small RNAs and the fertilization rate (FR), blastocyst rate and high-quality embryo rate (HQER). The sperm samples with high hsa-mir-191 expression had a higher FR, effective embryo rate (EER) and HQER. hsa-mir-191 was used as a single indicator to predict the HQER. The receiver operating characteristic (ROC) curve had an area under the ROC curve (AUC) of 0.686. We also found that hsa-mir-191 expression is correlated with an abnormal sperm rate (cor = 0.29, p< 0.01). We also evaluated the relationship between hsa-mir-34c and early human embryo development in these 102 sperm samples and obtained negative results. Conclusions: These findings suggest that high hsa-mir-191-5p expression in sperm is associated with early human embryonic quality and that hsa-mir-191-5p could be used as a potential marker to screen high-quality sperm to improve the success rates of in vitro fertilization (IVF).
Clinical analysis of Kimura’s disease in 24 cases from China
Authors: Zhang Guoliang , Li Xumao , Sun Guangbin , Cao Yitan , Gao Nan , Qi Weidong
Published: December 2019

Background: We reviewed details of Chinese Kimura’s disease (KD) cases. A full clinical analysis was subsequently performed to improve the accuracy of clinical diagnosis and treatment of KD.
Methods: A total of 24 patients with pathologically confirmed KD treated between March 2008 and March 2018 were reviewed retrospectively for clinical and histopathological analysis.
Results: In the 24 KD cases, 20 were male and 4 were female with the age of onset ranging from 5 to 65 years. Lesion diameter ranged from 0.6 cm to 7 cm with unilateral involvement being more popular (79%). Imaging examination had a high detection rate for KD involving the parotid gland and subcutaneous but had low specificity. Microscopic analysis indicated that KD mainly involved subcutaneous soft tissue and lymph nodes. The prominent feature of lymphoid tissue was germinal center hyperplasia surrounded by several lobules associated with hyperplastic vascular structures. Out of the 24 patients, 11 experienced recurrence of disease after treatment (surgical resection: 46.2%, surgical resection followed by oral corticosteroids: 71.4% and surgical resection combined with radiotherapy: 0%).
Conclusions: Our analysis revealed clinical, imaging, and histological characteristics of KD. A better understanding of the disease will help clinicians reduce misdiagnosis and improve the diagnostic rate upon patient first clinical visit.
The effectiveness of workplace nutrition and physical activity interventions in improving productivity, work performance and workability: A Systematic Review
Authors: Grimani Aikaterini , Aboagye Emmanuel , Kwak Lydia
Published: December 2019

Background: Healthy lifestyles play an important role in the prevention of premature death, chronic diseases, productivity loss and other social and economic concerns. However, workplace interventions to address issues of fitness and nutrition which include work-related outcomes are complex and thus challenging to implement and appropriately measure the effectiveness of. This systematic review investigated the impact of workplace nutrition and physical activity interventions, which include components aimed at workplace’s physical environment and organizational structure, on employees’ productivity, work performance and workability.
Methods: A systematic review that included randomized controlled trials and or non-randomized controlled studies was conducted. Medline, EMBASE.com, Cochrane Library and Scopus were searched until September 2016. Productivity, absenteeism, presenteeism, work performance and workability were the primary outcomes of our interest, while sedentary behavior and changes in other health-related behaviors were considered as secondary outcomes. Two reviewers independently screened abstracts and full-texts for study eligibility, extracted the data and performed a quality assessment using the Cochrane Collaboration Risk-of-Bias Tool for randomized trials and the Risk-of-Bias in non-randomized studies of interventions. Findings were narratively synthesized.
Results: Thirty-nine randomized control trials and non-randomized controlled studies were included. Nearly 28% of the included studies were of high quality, while 56% were of medium quality. The studies covered a broad range of multi-level and environmental-level interventions. Fourteen workplace nutrition and physical activity intervention studies yielded statistically significant changes on absenteeism (n=7), work performance (n=2), workability (n=3), productivity (n=1) and on both workability and productivity (n=1). Two studies showed effects on absenteeism only between subgroups.
Conclusions: The scientific evidence shows that it is possible to influence work-related outcomes, especially absenteeism, positively through health promotion efforts that include components aimed at the workplace’s physical work environment and organizational structure. In order to draw further conclusions regarding work-related outcomes in controlled high-quality studies, long-term follow-up using objective outcomes and/or quality assured questionnaires are required.
Registration number: PROSPERO CRD42017081837
Genome-wide association mapping revealed syntenic loci QFhb-4AL and QFhb-5DL for Fusarium head blight resistance in common wheat (Triticum aestivum L.)
Authors: Hu Wenjing , Gao Derong , Wu Hongya , Liu Jian , Zhang Chunmei , Wang Junchan , Jiang Zhengning , Liu Yeyu , Li Dongsheng , Zhang Yong , Lu Chengbin
Published: December 2019

Background: Fusarium head blight (FHB), primarily caused by Fusarium graminearum, is a major threat to wheat production and food security worldwide. Breeding stably and durably resistant cultivars is the most effective approach for managing and controlling the disease. The success of FHB resistance breeding relies on identification of an effective resistant germplasm. We conducted a genome-wide association study (GWAS) using the high-density wheat 90K single nucleotide polymorphism (SNP) assays to better understand the genetic basis of FHB resistance in natural population and identify associated molecular markers.
Results: The resistance to FHB fungal spread along the rachis (Type II resistance) was evaluated on 171 wheat cultivars in the 2016-2017 (abbr. as 2017) and 2017-2018 (abbr. as 2018) growing seasons. Using Illumina Infinum iSelect 90K SNP genotyping data, a genome-wide association study (GWAS) identified 26 loci (88 marker-trait associations), which explained 6.65-14.18% of the phenotypic variances. The associated loci distributed across all chromosomes except 2D, 6A, 6D and 7D, with those on chromosomes 1B, 4A, 5D and 7A being detected in both years. New loci for Type II resistance were found on syntenic genomic regions of chromsome 4AL (QFhb-4AL, 621.85-622.24 Mb) and chromosome 5DL (QFhb-5DL, 546.09-547.27 Mb) which showed high collinearity in gene content and order. SNP markers wsnp_JD_c4438_5568170 and wsnp_CAP11_c209_198467 of 5D, reported previously linked to a soil-borne wheat mosaic virus (SBWMV) resistance gene, were also associated with FHB resistance in this study.
Conclusion: The syntenic FHB resistant loci and associated SNP markers identified in this study are valuable for FHB resistance breeding via marker-assisted selection.
INSTITUTIONAL STRATEGIES AS A MECHANISM TO RATIONALIZE THE NEGATIVE EFFECTS OF JUDICIALIZATION OF ACCESS TO MEDICINE IN BRAZIL
Authors: Chagas Virginia Oliveira , Provin Mércia Pandolfo , Mota Pedro Augusto Prado , Guimarães Rafael Alves , Amaral Rita Goreti
Published: December 2019

Background: Recently, the Executive Branch and Judiciary in Brazil increased spending due to larger numbers of lawsuits that forced the State to provide health goods and services. This phenomenon, known as health judicialization, has created challenges and required the Executive Branch and Judiciary to create institutional strategies such as technical chambers and departments to reduce the social, economic and political distortions caused by this phenomenon. This study aims to evaluate the effects of two institutional strategies deployed by a Brazilian municipality in order to cope with the economic, social and political distortions caused by the phenomenon of health judicialization regarding access to medicines. Methods: A longitudinal study was carried out in a capital in the Central-West Region of Brazil. A sample of 511 lawsuits was analyzed. The variables were placed into three groups: the sociodemographic characteristics and the plaintiffs’ disease, the characteristics of the claimed medical products and the institutional strategies. To analyze the effect of the interventions on the total cost of the medicines in the lawsuits, bivariate and multivariate linear regressions with variance were performed. For the categorical outcomes, Poisson regressions were performed with robust variance, using a significance level of 5%. Results: A reduction in the costs of medicines in the lawsuits and of the requests for medicines within the SUS formulary was verified after the deployment of the Department of Assessment of Non-Standardized Medicines (DAMNP) and the Technical Chamber of Health Assessment (CATS); an increase in processed prescriptions from the Brazilian Universal Health System was observed after the deployment of the CATS; and an increase in medicines outside the SUS formulary without a therapeutic alternative was verified after the CATS. Conclusion: The institutional strategies deployed were important tools to reduce the high costs of the medicines in the lawsuits. In addition, they represented a step forward for the State, provided a benefit to society and indicated a potential path for the health and justice systems of other countries that also face problems caused by the judicialization of health.
Genome-wide analysis of wheat DNA-binding with one finger (Dof) transcription factor genes: evolutionary characteristics and diverse abiotic stress responses
Authors: Liu Yue , Liu Nannan , Deng Xiong , Liu Dongmiao , Li Mengfei , Cui Dada , Hu Yingkao , Yan Yueming
Published: December 2019

Background: DNA binding with one finger (Dof) transcription factors play important roles in plant growth and abiotic stress responses. Although genome-wide identification and analysis of the DOF transcription factor family has been reported in other species, no relevant studies have emerged in wheat. The aim of this study was to investigate the evolutionary and functional characteristics associated with plant growth and abiotic stress responses by genome-wide analysis of the wheat Dof transcription factor gene family.
Results: Using the recently released wheat genome database (IWGSC RefSeq v1.1), we identified 96 wheat Dof gene family members, which were phylogenetically clustered into five distinct subfamilies. Gene duplication analysis revealed a broad and heterogeneous distribution of TaDofs on the chromosome groups 1 to 7, possibly related to segmental duplication and tandem duplication. Members of the same gene subfamily had similar exon-intron structures, while members of different subfamilies had obvious differences. Functional divergence analysis indicated that type-II functional divergence played a major role in the differentiation of the TaDof gene family. Positive selection analysis revealed that the Dof gene family experienced different degrees of positive selection pressure during the process of evolution, and five significant positive selection sites (30A, 31T, 33A, 102G and 104S) were identified. Additionally, nine groups of coevolving amino acid sites, which may play a key role in maintaining the structural and functional stability of Dof proteins, were identified. The results from the RNA-seq data and qRT-PCR analysis revealed that TaDof genes exhibited obvious expression preference or specificity in different organs and developmental stages, as well as in diverse abiotic stress responses. Most TaDof genes were significantly upregulated by heat, PEG and heavy metal stresses.
Conclusions: The genome-wide analysis and identification of wheat DOF transcription factor family and the discovery of important amino acid sites are expected to provide new insights into the structure, evolution and function of the plant Dof gene family.
Immune-related miRNA-mRNA regulation network in the livers of DHAV-3-infected ducklings
Authors: Wu Fengyao , Lu Fengying , Fan Xin , Chao Jin , Liu Chuanmin , Pan Qunxing , Sun Huawei , Zhang Xiaofei
Published: December 2019

Background: Duck hepatitis A virus type 3 (DHAV-3) is one of the most harmful pathogens in the duck industry. However, the molecular mechanism underlying DHAV-3 infection in ducklings is remain poorly understood. To elucidate the genetic regulatory network for miRNA-mRNA and the signaling pathways involved in DHAV-3 infection in ducklings, we conducted global miRNA and mRNA expression profiling of duckling liver tissues infected with lethal DHAV-3 using high-throughput sequencing. Results: We found 156 differentially expressed miRNAs (DEMs) and 7717 differentially expressed miRNAs (DEGs) between mock-infected and DHAV-3-infected duckling livers. A total of 19,606 miRNA-mRNA pairs with negatively correlated expression patterns were identified in miRNA-mRNA networks constructed on the basis of these DEMs and DEGs. Moreover, immune-related pathways including the cytokine-cytokine receptor interaction, apoptosis, Toll-like receptor, Jak-STAT, and RIG-I-like receptor signaling pathway were significantly enriched through analyzing functions of mRNAs in the network in response to DHAV-3 infection. Besides, apl-miR-32-5p, apl-miR-125-5p, apl-miR-128-3p, apl-miR-460-5p, and novel-m0012-3p were identified as potential regulators in the immune-related signaling pathways during the DHAV-3 infection. Conclusions: To our knowledge, this is the first report on integrated analysis of miRNA-seq and mRNA-seq in DHAV-3-infected ducklings. The results indicated the important roles of miRNAs in regulating immune response genes and revealed the immune related miRNA-mRNA regulation network in the DHAV-3-infected duckling liver. Our findings may provide valuable information to further investigate the roles of miRNAs and their target genes in DHAV-3 replication and pathogenesis; additionally, they may offer clues for further understanding host-virus interactions.
Are countries’ self-reported assessments of their capacity for infectious disease control reliable? Associations among countries’ self-reported International Health Regulation 2005 capacity assessments and infectious disease control outcomes
Authors: Tsai feng-jen , Tipayamongkholgul Mathuros
Published: December 2019

Background This study aimed to evaluate associations among countries’ self-reported International Health Regulation 2005 (IHR 2005) capacity assessments and infectious disease control outcomes. Methods Countries’ self-reported assessments implemented by percentages as IHR Monitoring Tools (IHRMT) in 2016 and 2017 were used to represent national capacity regarding infectious disease control. WHO Disease Outbreak News and matched diseases reports on ProMED-mail were collected in 2016 to represent disease control outcomes of countries. Disease control outcomes were divided in good, normal and bad groups based on the development of outbreaks listed in the reports. The Human Development Index (HDI), density of physicians and nurses, health expenditure, number of arrivals of international tourists were also collected for control. Chi-square test and logistic regression were applied for analysis. Results A total of 907 cases occurred in 92 countries. For all diseases, cases occurring in high international travel volume countries presented twice the risk of having a bad disease control outcomes than cases occurring in low international travel volume countries (OR = 2.19 for IHR 2016, OR =2.97 for IHR 2017). Cases occurring in low IHR average score countries had significant higher risk (OR = 7.83 for IHR 2016 and OR = 2.23 for IHR 2017) of having a bad disease control outcomes than countries with high IHR average scores. For only human diseases, cases occurring in high international travel volume countries presented twice the risk of having a bad disease control outcomes than cases occurring in low international travel volume countries for IHR 2017 (OR =2.79). Cases occurring in low IHR average score countries had significant higher risk (OR = 11.16 for IHR 2016 and OR = 3.45 for IHR 2017) of having a bad disease control outcomes than countries with high IHR average scores. The HDI, health workforce density and total health expenditure were all positively associated with disease control outcomes. Conclusions Countries’ self-reported infectious disease control capacities positively correlated with their disease control outcomes. While the self-reported IHR scores were accountable to some degree, this approach was useful for understanding global capacity in infectious disease control and in allocating resources for future preparedness.