bluuebunny/crossref_metadata_2025 huggingface dataset:
When social movements fail or succeed: social psychological consequences of a collective action’s outcome
Authors: Carvacho Héctor , González Roberto , Cheyre Manuel , Rocha Carolina , Cornejo Marcela , Jiménez-Moya Gloria , Manzi Jorge , Álvarez-Dezerega Catalina , Álvarez Belén , Castro Diego , Varela Micaela , Valdenegro Daniel , Drury John , Livingstone Andrew
Published: April 2023

Collective actions occur all around the world and, in the last few years, even more frequently. Previous literature has mainly focused on the antecedents of collective actions, but less attention has been given to the consequences of participating in collective action. Moreover, it is still an open question how the consequences of collective action might differ, depending on whether the actions are perceived to succeed or fail. In two studies we seek to address this gap using innovative experimental studies. In Study 1 (N = 368) we manipulated the perceptions of success and failure of a collective action in the context of a real social movement, the Chilean student movement from last decade. In Study 2 (N = 169), in addition to manipulating the outcome, we manipulated actual participation, using a mock environmental organization aiming to create awareness in authorities, to test the causal effect of both participation and success/failure on empowerment, group efficacy, and intentions of future involvement in normative and non-normative collective actions. Results show that current and past participation predict overall participation in the future, however, in Study 2 the manipulated participation was associated with having less intentions of participating in the future. In both studies, perception of success increases group efficacy. In Study 1, we found that when facing failure, participants increase their willingness to participate more in the future as opposed to non-participants that actually decrease theirs. In Study 2, however, failure increases the perception of efficacy for those with a history of non-normative participation. Altogether these results highlight the moderating role of the outcome of collective action to understand the effect of participation on future participation. We discuss these results in light of the methodological innovation and the real world setting in which our studies were conducted.
Intrinsic drive of medical staff: a survey of employee representatives from 22 hospitals in China
Authors: Zhang Yuqing , Yuan Zheng , Cheng Taozhu , Wang Cunliang , Li Jun
Published: April 2023

Objective

While several initiatives, including monetary rewards and performance system reform, are used to inspire medical staff, none are fully effective. We sought to describe the intrinsic drive of medical staff and identify elements that improve work enthusiasm by increasing internal motivation.

Methods

A cross-sectional study was conducted in which 2,975 employee representatives from 22 municipal hospitals in Beijing, China were interviewed using a self-made intrinsic motivation scale for medical staff which includes the achievement motivation, self-efficacy, conscientiousness, gratitude level and perceived organizational support. The Kruskal-Wallis analysis of variance and multiple linear regression methods were used to investigate the level of intrinsic motivation and identify any influencing factors. The correlation between employee drive and turnover intention was determined using Spearman rank correlation analysis and Kendall’s tau b rank correlation coefficient.

Results

A total of 2,293 valid answers were obtained, with a valid recovery rate of 77.1%. There were statistically significant differences in intrinsic motivation and its five dimensions by marital status, political status, profession, service year, monthly income, number of working hours per week, and turnover intention (p < 0.05). Being divorced, a CPC member, in the nursing profession, and having a higher monthly income had a positive impact on intrinsic motivation while working a high hours per week had a negative effect. Higher work drive was associated with lower turnover intention. The correlation coefficients of intrinsic drive and its five dimensions with turnover intention ranged from 0.265 to 0.522 (p < 0.001).

Conclusion

Sociodemographic factors and work environment influenced the intrinsic motivation of medical staff. There was a correlation between work drive and turnover intention which indicated that stimulating the intrinsic drive of employees may help to increase staff retention.
Norms and T-scores for screeners of alcohol use, depression and anxiety in the population of Suriname
Authors: de Beurs Edwin , Jadnanansing Raj , Etwaroo Kajal , Blankers Matthijs , Bipat Robbert , Peen Jaap , Dekker Jack
Published: April 2023

Background

There is a considerable gap between care provision and the demand for care for common mental disorders in low-and-middle-income countries. Screening for these disorders, e.g., in primary care, will help to close this gap. However, appropriate norms and threshold values for screeners of common mental disorders are lacking.

Methods

In a survey study, we gathered data on frequently used screeners for alcohol use disorders, (AUDIT), depression, (CES-D), and anxiety disorders (GAD-7, ACQ, and BSQ) in a representative sample from Suriname, a non-Latin American Caribbean country. A stratified sampling method was used by random selection of 2,863 respondents from 5 rural and 12 urban resorts. We established descriptive statistics of all scale scores and investigated unidimensionality. Furthermore, we compared scores by gender, age-group, and education level with t-test and Mann–Whitney U tests, using a significance level of p < 0.05.

Results

Norms and crosswalk tables were established for the conversion of raw scores into a common metric: T-scores. Furthermore, recommended cut-off values on the T-score metric for severity levels were compared with international cut-off values for raw scores on these screeners.

Discussion

The appropriateness of these cut-offs and the value of converting raw scores into T-scores are discussed. Cut-off values help with screening and early detection of those who are likely to have a common mental health disorder and may require treatment. Conversion of raw scores to a common metric in this study facilitates the interpretation of questionnaire results for clinicians and can improve health care provision through measurement-based care.
Randomized controlled pilot study of an app-based intervention for improving social skills, face perception, and eye gaze among youth with autism spectrum disorder
Authors: Chung Kyongmee , Chung Eunsun
Published: April 2023

Introduction

This pilot study aimed to examine the effectiveness of an app-based intervention (Yface) in 53 children with autism spectrum disorder. Yface is a combined program that improves social skills, facial perception, and eye gaze.

Methods

Children were randomly assigned to one of the two training groups or a waitlist control group. One of the training groups completed the Yface training program lasting for 66 days, while the other training group used a similar app called Ycog, which focuses on cognitive rehabilitation. Questionnaires, computerized tasks, and semi-structured interviews were administered to children and their parents at pre- and post-training sessions.

Results

The Yface group showed improvements in face perception and some social skills compared to waitlist controls, and in eye gaze compared to the Ycog group.

Discussion

Our results suggest that this app-based intervention is effective in improving targeted social skills and face perception, although their relative effectiveness differs across skill domains.
Can integrating religiosity and spirituality into postpartum care improve the quality of life in women with preeclampsia
Authors: Rasouli Robab , Maleki Azam , Zenoozian Saeedeh
Published: April 2023

Background

Women with a history of preeclampsia frequently have a lower level of physical well-being and emotional problems.

Objective

This study aimed to determine the effect of integrating religiosity and spirituality into postpartum care can improve the quality of life in women with preeclampsia.

Methods

This study was a randomized controlled clinical trial conducted on 40 women with preeclampsia. All eligible participants were allocated to two control and intervention groups using a random blocking method. Data were collected using Mother-Generated Index (MGI) in pre-intervention and 6 weeks later and analyzed using descriptive statistics, Chi-square test, and independent t-tests. The significance level was p < 0.05.

Results

The mean, Standard deviation (SD) of the total score of MGI before intervention in the intervention group was 5.35 (1.09) which increased to 8.00 (0.50) 6 weeks after intervention. In the control group, the pre-test score of MGI was 5.81 (0.97) which increased to 6.69 (1.37) after 6 weeks of follow-up. The difference between the two groups was statistically significant after the intervention based on an independent t-test (p = 0.001).

The mean (SD) of five subscales included Feelings toward herself, Feelings toward the child, Feelings toward her husband and others, Feelings toward sex, and Physical health status after intervention in the intervention group statistically significantly increased compared to the control group (p < 0.011).

Conclusion

The integration of spiritual counseling with the educational content of postpartum care had a positive impact on improving the postpartum QoL of women with preeclampsia. For better conclusions, a study with a large sample size needed to be conducted in the future.

Clinical Trial Registration

https://en.irct.ir/user/trial/50832/view, identifier IRCT20150731023423N16.
Knowledge, attitude, and adherence to malaria control guidelines and the prevalence of Plasmodium species infection in localities across transmission and ecological zones in Cameroon
Authors: Tangi Livinus N. , Ajonina Marcelus U. , Moyeh Marcel N. , Chi Hanesh F. , Ntui Vincent N. , Kwi Pilate N. , Toussi Eric C. T. , Fung Mary Progress S. , Fah FohTella , Mayaba Joel M. , Formilack Franklin T. , Ntasin Veronica N. , Nji Theobald M. , Yenshu Emmanuel V. , Achidi Eric A. , Amambua-Ngwa Alfred , Apinjoh Tobias O.
Published: April 2023

Background

Despite a scale up of control interventions over the years, malaria remains a major public health and economic concern in Cameroon, contributing considerably to hospitalization and deaths. The effectiveness of control strategies depends on the extent of adherence by the population to national guidelines. This study assessed the influence of human knowledge, attitudes, and practices related to malaria and its control on the prevalence of malaria parasite infection, with implications for the elimination of the disease.

Methodology

This is a cross-sectional community and hospital-based study, covering the five ecological and three malaria transmission zones in Cameroon. A pre-tested semi-structured questionnaire was used to document socio-demographic and clinical parameters as well as knowledge, attitudes, and practices toward malaria control and management. Consenting participants were screened for malaria parasite with rapid diagnostic test (mRDT) of the peripheral blood. Association between qualitative variables was determined using the chi-square test and logistic regression analysis.

Results

A total of 3,360 participants were enrolled, 45.0% (1,513) of whom were mRDT positive, with 14.0% (451/3,216) and 29.6% (951/3,216) having asymptomatic parasitaemia and malaria, respectively. Although most participants knew the cause, symptoms, and control strategies, with 53.6% (1,000/1,867) expertly knowledgeable about malaria overall, only 0.1% (2/1,763) individuals were fully adherent to malaria control measures.

Conclusion

The risk of malaria in Cameroon remains high, with the population considerably knowledgeable about the disease but poorly adherent to national malaria control guidelines. Concerted and more effective strategies aimed at improving knowledge about malaria and adherences to control interventions are necessary to ultimately eliminate the disease.
Legal linguistic templates and the tension between legal knowledge representation and reasoning
Authors: Libal Tomer
Published: April 2023

There is an inherent tension between knowledge representation and reasoning. For an optimal representation and validation, an expressive language should be used. For an optimal automated reasoning, a simple one is preferred. Which language should we choose for our legal knowledge representation if our goal is to apply automated legal reasoning? In this paper, we investigate the properties and requirements of each of these two applications. We suggest that by using Legal Linguistic Templates, one can solve the above tension in some practical situations.
Participation, challenges and needs in children with down syndrome during cancer treatment at hospital: a qualitative study of parents' experiences
Authors: Bohnstedt Cathrine , Stenmarker Margaretha , Olersbacken Linn , Schmidt Lone , Larsen Hanne B. , Schmiegelow Kjeld , Hansson Helena
Published: April 2023

Background

Studies report that it can be challenging to assess and treat side-effects and symptoms among children who have impairments and difficulties in expressing their needs. Children with Down syndrome have an increased vulnerability and an increased risk for contracting leukaemia. There is sparse knowledge about the parental experience of how treatment and side-effects affect children with Down syndrome with leukaemia, as well as the role of participation during treatment.

Purpose

This study aimed to explore the perceptions of parents of children with Down syndrome and leukaemia regarding their child's treatment, side effects and participation during hospital care.

Methods

A qualitative study design was used, and interviews were conducted with a semi-structured interview-guide. Fourteen parents of 10 children with Down syndrome and acute lymphoblastic leukaemia from Sweden and Denmark, 1–18 years of age, participated. All children had completed therapy or had a few months left before the end of treatment. Data was analysed according to qualitative content analysis.

Results

Four sub-themes were identified: (1) Continuously dealing with the child's potential susceptibility; (2) Confidence and worries regarding decisions related to treatment regulation; (3) Challenges in communication, interpretation, and participation; and (4) Facilitating participation by adapting to the child's behavioural and cognitive needs. The sub-themes were bound together in an overarching theme, which expressed the core perception “Being the child's spokesperson to facilitate the child's participation during treatment”. The parents expressed this role as self-evident to facilitate communication regarding the needs of the child, but also regarding how the cytotoxic treatment affected the vulnerable child. Parents conveyed the struggle to ensure the child's right to receive optimal treatment.

Conclusion

The study results highlight parental challenges regarding childhood disabilities and severe health conditions, as well as communication and ethical aspects regarding to act in the best interests of the child. Parents played a vital role in interpreting their child with Down syndrome. Involving parents during treatment enables a more accurate interpretation of symptoms and eases communication and participation. Still, the results raise questions regarding issues related to building trust in healthcare professionals in a context where medical, psychosocial and ethical dilemmas are present.
Food justice accompaniment research: theory and social praxis in West Virginia
Authors: Wilson Bradley R. , Lohnes Joshua
Published: April 2023

Over the past three decades West Virginians have experienced a deepening economic crisis. Divestment in coal and manufacturing has resulted in widespread unemployment, state, county and municipal revenue losses, and cascading effects on social services, households, livelihoods and community life. For 10 years, FJL has conducted ethnographic research, coordinated cooperative experiments, and built pedagogical tools to democratize knowledge about West Virginia’s food system amidst this crisis. Working in a so-called red state, we have fostered conversations about food justice with rural, often socially conservative communities, and have worked to raise up human resources for meaningful community-led food justice organizing in Central Appalachia. In this paper, we consider the long accompaniment process with community partners and the effects of this experience on the evolution of research questions and actions.
The global status and hotspots of research in the field of trans-oral endoscopic thyroidectomy (TOET) from 2008 to 2022
Authors: Li Ping , Qin Hao , Jin Rui , Zheng Wuping , Fan Pingming , Lyu Peng-fei
Published: April 2023

Purpose

In this study, the aim was to comprehensively analyze the current status, hotspots, and trends of trans-oral endoscopic thyroidectomy (TOET) through bibliometric analysis and by presenting the field atlas.

Methods

Web of Science Core Collection database was adopted to screen studies regarding TOET published between January 1, 2008 and August 1, 2022. The evaluation covered the criteria total number of studies, keywords, and contributions from countries/regions, institutions, journals, and authors.

Results

A total of 229 studies were covered. SURGICAL ENDOSCOPY AND OTHER INTERVENTIONAL TECHNIQUES is the largest publication in the field of TOET. The three countries that contributed the most studies were Korea, China, and the USA. The most frequently occurring core keywords in the field of TOET are vestibular approach, outcomes, experience, safety, robotic thyroidectomy, scar, video-assisted thyroidectomy and quality-of-life. The seven clusters were generated in this study: intraoperative monitoring of the laryngeal return nerve (# 0), learning curve (# 1), postoperative quality of life (# 2), central lymph node dissection and safety (# 3), complications (# 4), minimally invasive surgery (# 5), and robotic surgery (# 6).

Conclusion

The main research topics in the field of TOET place focuses on learning curves, laryngeal nerve monitoring, carbon dioxide gas bolus, chin nerve injury, surgical complications, and surgical safety. In the future, more academics will focus on the safety of the procedure and reducing complications..
Internal replacement of a vertebral body in pseudarthrosis—Armed kyphoplasty with bone graft-filled stents: Case report
Authors: Moura Diogo Lino , Cavaca Ana Rita
Published: April 2023

Background

Post-traumatic vertebral necrosis and pseudarthrosis represents one of the most concerning and unpredictable challenges in spinal traumatology. The evolution of this disease at the thoracolumbar transition usually courses with progressive bone resorption and necrosis, leading to vertebral collapse, retropulsion of the posterior wall and neurological injury. As such, the therapeutic goal is the interruption of this cascade, seeking to stabilize the vertebral body and avoid the negative consequences of its collapse.

Case description

We present a clinical case of a pseudarthrosis of T12 vertebral body with severe posterior wall collapse, treated with removal of intravertebral pseudarthrosis focus by transpedicular access, T12 armed kyphoplasty with VBS® stents filled with cancellous bone autograft, laminectomy and stabilization with T10-T11-L1-L2 pedicle screws. We present clinical and imaging detailed results at 2-year follow-up and discuss our option for this biological minimally invasive treatment for vertebral pseudarthrosis that mimics the general principles of atrophic pseudarthrosis therapeutic and allows to perform an internal replacement of the necrotic vertebral body, avoiding the aggression of a total corpectomy.

Conclusions

This clinical case demonstrates a successful outcome of the surgical treatment of pseudarthrosis of vertebral body (mobile nonunion vertebral body) in which expandable intravertebral stents allow to perform an internal replacement of the necrotic vertebral body by creating intrasomatic cavities and filling them with bone graft, obtaining a totally bony vertebra with a metallic endoskeleton, which is biomechanically and physiologically more similar to the original one. This biological internal replacement of the necrotic vertebral body technique can be a safe and effective alternative over cementoplasty procedures or total vertebral body corpectomy and replacement for vertebral pseudarthrosis and may have several advantages over them, however long-term prospective studies are needed in order to prove the effectiveness and advantages of this surgical option in this rare and difficult pathological entity.
Modelling Proper and Improper Sitting Posture of Computer Users Using Machine Vision for a Human–Computer Intelligent Interactive System during COVID-19
Authors: Estrada Jheanel E. , Vea Larry A. , Devaraj Madhavi
Published: April 2023

Human posture recognition is one of the most challenging tasks due to the variation in human appearance, changes in the background and illumination, additional noise in the frame, and diverse characteristics and amount of data generated. Aside from these, generating a high configuration for recognition of human body parts, occlusion, nearly identical parts of the body, variations of colors due to clothing, and other various factors make this task one of the hardest in computer vision. Therefore, these studies require high-computing devices and machines that could handle the computational load of this task. This study used a small-scale convolutional neural network and a smartphone built-in camera to recognize proper and improper sitting posture in a work-from-home setup. Aside from the recognition of body points, this study also utilized points’ distances and angles to help in recognition. Overall, the study was able to develop two objective datasets capturing the left and right side of the participants with the supervision and guidance of licensed physical therapists. The study shows accuracies of 85.18% and 92.07%, and kappas of 0.691 and 0.838, respectively. The system was developed, implemented, and tested in a work-from-home environment.
Effect of Water-Soluble CMC/SBR Binder Ratios on Si-rGO Composites Using µm- and nm-Sized Silicon as Anode Materials for Lithium-Ion Batteries
Authors: Müllner Sebastian , Michlik Tobias , Reichel Michael , Held Tilo , Moos Ralf , Roth Christina
Published: April 2023

Silicon-containing materials are still the most promising alternatives to graphite as the negative electrodes of lithium-ion batteries. However, the different Li+ storage mechanism combined with the high capacity result in new requirements for the passive electrode components, such as the binder. To ensure sufficient cycling stability, silicon must be embedded in a suitable carbonaceous matrix. For this purpose, we used a simple ball milling process with reduced graphene oxide (rGO) to produce Si-rGO composites with µm- and nm-sized silicon particles. The rGO was synthesized previously from a two-step thermal synthesis method developed in-house. Subsequently, electrodes with varying CMC/SBR ratios (3:1, 1:1, and 1:3) were prepared from the composites containing the different Si particle sizes. It was found that the optimal binder ratio depends on the size of the Si particles. For the nm-Si-rGO composite, a CMC/SBR ratio of 3:1 results in a total capacity over 51 cycles of 20.6 Ah g−1, which means an improvement of 20% compared to CMC/SBR = 1:3 (17.1 Ah g−1). In contrast, we demonstrate that for µm-Si-rGO composites with an optimal CMC/SBR ratio of 1:1 (13.0 Ah g−1), compared to nm-Si-rGO, a higher SBR content is beneficial for the cycling behavior. Moreover, a comparison with graphite from the literature indicates that a rGO-matrix reduces the need for SBR.
Switching between Originators and Biosimilars in Dermatology: A Systematic Review of Real-World Clinical Studies
Authors: Nicoletti Maria Maddalena , Crisci Erminia , Pentella Ciro , Cantone Andrea , Ruggiero Donatella , Anatriello Antonietta , Scavone Cristina
Published: April 2023

Background. Although biosimilars have been increasingly used over recent years, some concerns about a potential loss of efficacy and altered safety profile when switching from an originator to a biosimilar still exist. Interchangeability can be a challenge for dermatologists too. An extensive systematic review of published switching studies among originators and biosimilars was carried out in order to provide evidence regarding the effects derived from the switch in terms of efficacy and safety outcomes in real-life contexts. Results. Thirty-seven articles were included in this systematic review (14 studies related to adalimumab, 10 to etanercept, 12 to infliximab, and 1 each to adalimumab, etanercept, and infliximab). Studies were mainly carried out among European countries. Most of them were observational studies or register-based studies. The majority of studies enrolled patients diagnosed with psoriasis or psoriatic arthritis who underwent a single switch from the originator to the biosimilar. Overall, the studies’ results demonstrated that switching between adalimumab, etanercept, and infliximab originators and biosimilars is safe and effective in a real-life setting of patients with dermatological conditions. Only a few studies highlighted an increase in the risk of loss of efficacy as well as an increased rate of AEs, both of which were identified as the main causes of biosimilar discontinuation, probably associated with the well-known phenomenon of the nocebo effect. Conclusion. Switching from a biologic originator to its biosimilar is safe and effective. Only a few studies have evaluated the switch among biosimilars; thus, no firm conclusion can be drawn for this type of switch in terms of the efficacy and safety outcomes. Based on our results, we believe that biosimilars can be considered interchangeable with their reference products and that no additional switch studies are necessary to support switching among originators and biosimilars in clinical practice. However, the continuous monitoring of all biologics (both originators and biosimilars) in routine clinical practice is strongly needed given their peculiar safety profile.
A 512-Ch Dual-Mode Microchip for Simultaneous Measurements of Electrophysiological and Neurochemical Activities
Authors: Mulberry Geoffrey , White Kevin , Crocker Matthew , Kim Brian
Published: April 2023

In the study of the brain, large and high-density microelectrode arrays have been widely used to study the behavior of neurotransmission. CMOS technology has facilitated these devices by enabling the integration of high-performance amplifiers directly on-chip. Usually, these large arrays measure only the voltage spikes resulting from action potentials traveling along firing neuronal cells. However, at synapses, communication between neurons occurs by the release of neurotransmitters, which cannot be measured on typical CMOS electrophysiology devices. Development of electrochemical amplifiers has resulted in the measurement of neurotransmitter exocytosis down to the level of a single vesicle. To effectively monitor the complete picture of neurotransmission, measurement of both action potentials and neurotransmitter activity is needed. Current efforts have not resulted in a device that is capable of the simultaneous measurement of action potential and neurotransmitter release at the same spatiotemporal resolution needed for a comprehensive study of neurotransmission. In this paper, we present a true dual-mode CMOS device that fully integrates 256-ch electrophysiology amplifiers and 256-ch electrochemical amplifiers, along with an on-chip 512 electrode microelectrode array capable of simultaneous measurement from all 512 channels.
Quantum Deep Learning for Fast Switching of Full-Bridge Power Converters
Authors: Gheisarnejad Meysam , Khooban Mohammad-Hassan
Published: April 2023

With the qualitative development of DC microgrids, the usage of different loads with unique conditions and features is now possible in electric power grids. Due to the negative impedance features of some loads, which are called constant power loads (CPLs), the control of DC power converters faces huge challenges from a stability point of view. Despite the significant advances in semiconductors, there is no upgrade in the control of gate drivers to exploit all potential of power electronic systems. In this paper, quantum computations are incorporated into artificial intelligence (AI) to stabilize a full-bridge (FB) DC-DC boost converter feeding CPL. Aiming to improve the bus voltage stabilization of the FB DC-DC boost converter, a quantum deep reinforcement learning (QDRL) control methodology is developed. By defining a reward function according to the specification of the FB power converter, the desired performance and control objectives are fulfilled. The main task of QDRL is to adjust the control coefficients embedded in the feedback controller to suppress the negative impedance effect resulting from deploying the CPLs. By deploying the potential advantages of quantum fundamentals, the deep reinforcement learning improved by quantum specifications will not only enhance the performance of the DRL algorithm on conventional processes but also advance related research areas such as quantum computing and AI. Unlike the basic quantum theory, which requires real quantum hardware, QDRL can be executed on classic computers. To examine the feasibility of the QDRL scheme, hardware-in-the-loop (HiL) examinations are conducted using the OPAL-RT. The comparison of the proposed controller with the classic state-of-the-art methodologies reveals the superiority and feasibility of QDRL-based control schemes in both the transient and steady-state conditions to other schemes. Analysis using various performance criteria, including the integral absolute error (IAE), integral time absolute error (ITAE), mean absolute error (MAE), and root mean square error (RMSE), demonstrates the dynamic improvement of the proposed scheme over sliding mode control (approximately 50%) and proportional integral control (approximately 100%).
An Experimental EMC Investigation of On-Board Interleaved Buck Converters
Authors: Nagy Lajos , Kőnig Tamás
Published: April 2023

On board a satellite, there are different devices that operate from a common energy source called a power bus. Since there are many devices connected to the same power bus and they are in the close vicinity of each other, it is important that they are able to function adequately in the electromagnetic environment of the satellite without introducing intolerable electromagnetic interference to other devices. On-board devices often require voltage levels different from the bus voltage to function, and this is often produced by switching-mode power supplies (SMPS). Their switching-mode operation guarantees that there is less power loss during conversion; however, they also emit conducted and radiated noises into the direction of the power bus and the payloads. In the case of multiple SMPS powered by the same power bus, we can interleave their switching periods. Thus, their noises will cancel each other out on their common input. In this study, we are going to demonstrate the noise reduction of the interleaving method with a setup consisting of two DC-DC converters connected to a simulated power bus.
An Overview of Extreme Years in Quercus sp. Tree Ring Records from the Northern Moldavian Plateau
Authors: Nagavciuc Viorica , Mursa Andrei , Ionita Monica , Sfeclă Victor , Popa Ionel , Roibu Cătălin-Constantin
Published: April 2023

In this study, we made use of a regional oak tree-ring network from six stands that cover the northern Moldavian Plateau (eastern Europe) to analyze how different tree ring parameters (i.e., early wood tree-ring width, late wood tree-ring width, and total tree-ring width) of Quercus sp. are influenced by the occurrence of extreme climatic events (e.g., long-lasting drought events). In order to explore the influence of extreme hydroclimatic events on tree ring width, we have selected each of the six most extreme positive and negative years of tree growth and addressed the seasonal cycle of tree growth in comparison with the main climatic parameters, then evaluated both the current and lagged consequences of extreme hydroclimatic events on tree ring width and the capacity of trees to recover. Our results indicate that the variability of oak tree ring width from the Moldavian Plateau is mainly influenced by the availability of water resources, and that an important limiting growth factor for Quercus sp. is the occurrence of long-lasting drought events, e.g., at least two years in a row with severe drought conditions.
Fast Linear Canonical Transform for Nonequispaced Data
Authors: Sun Yannan , Qian Wenchao
Published: April 2023

The investigations of the discrete and fast linear canonical transform (LCT) are becoming one of the hottest research topics in modern signal processing and optics. Among them, the fast calculation of LCT for non-uniform data is one of key problems. Focus on this problem, a new fast algorithm of the LCT has been proposed in this paper firstly by interpolation and approximation theory. The proposed algorithms can calculate quickly the LCT of the data, whether the input or output data is uniform. Secondly, the complexity and precision of derived algorithms have been analyzed for different situations. Finally, the experimental results are presented to verify the correctness of the obtained results.
2D Numerical Simulation of Floods in Ebro River and Analysis of Boundary Conditions to Model the Mequinenza Reservoir Dam
Authors: Vallés Pablo , Echeverribar Isabel , Mairal Juan , Martínez-Aranda Sergio , Fernández-Pato Javier , García-Navarro Pilar
Published: April 2023

The computational simulation of rivers is a useful tool that can be applied in a wide range of situations from providing real time alerts to the design of future mitigation plans. However, for all the applications, there are two important requirements when modeling river behavior: accuracy and reasonable computational times. This target has led to recent developments in numerical models based on the full two-dimensional (2D) shallow water equations (SWE). This work presents a GPU accelerated 2D SW model for the simulation of flood events in real time. It is based on a well-balanced explicit first-order finite volume scheme able to run over dry beds without the numerical instabilities that are likely to occur when used in complex topography. The model is applied to reproduce a real event in the reach of the Ebro River (Spain) with a downstream reservoir, in which a study of the most appropriate boundary condition (BC) for modeling of the dam is assessed (time-dependent level condition and weir condition). The whole creation of the model is detailed in terms of mesh optimization and validation. The simulation results are compared with field data over the flood duration (up to 20 days), allowing an analysis of the performance and time saved by different GPU devices and with the different BCs. The high values of fit between observed and simulated results, as well as the computational times achieved, are encouraging to propose the use of the model as a forecasting system.
Static and Dynamical Quantum Studies of CX3-AlX2 and CSiX3-BX2 (X = F, Cl, Br) Complexes with Hydrocyanic Acid: Unusual Behavior of Strong π-Hole at Triel Center
Authors: Michalczyk Mariusz , Wojtkowiak Kamil , Panek Jarosław J. , Jezierska Aneta , Zierkiewicz Wiktor
Published: April 2023

The set of TX3-TrX2 (T = C, Si, Ge; Tr = B, Al, Ga; X = F, Cl, Br) molecules offers a rather unique opportunity to study both σ-hole and π-hole dimerization on the tetrel and triel ends, respectively. According to the molecular electrostatic potential (MEP) distribution, the π-hole extrema (acidic sites) were more intense than their σ-hole counterparts. The molecules owning the most (CX3-AlX2) and least (SiX3-BX2) intense π-holes were chosen to evaluate their capacities to attract one and two HCN molecules (Lewis bases). We discovered that the energetic characteristics of π-hole dimers severely conflict with the monomers MEP pattern since the weakest π-hole monomer forms a dimer characterized by interaction energy compared to those created by the monomers with noticeably greater power in the π-hole region. This outcome is due to the deformation of the weakest π-hole donor. Furthermore, the MEP analysis for monomers in the geometry of respective dimers revealed a “residual π-hole” site that was able to drive second ligand attachment, giving rise to the two “unusual trimers” examined further by the NCI and QTAIM analyses. Apart from them, the π-hole/π-hole and σ-hole/π-hole trimers have also been obtained throughout this study and described using energetic and geometric parameters. The SAPT approach revealed details of the bonding in one of the “unusual trimers”. Finally, Born-Oppenheimer Molecular Dynamics (BOMD) simulations were carried out to investigate the time evolution of the interatomic distances of the studied complexes as well as their stability.
SAMHD1 Attenuates Acute Inflammation by Maintaining Mitochondrial Function in Macrophages via Interaction with VDAC1
Authors: Xu Bowen , Sui Qianyi , Hu Han , Hu Xiangjia , Zhou Xuchang , Qian Cheng , Li Nan
Published: April 2023

Over-activation of Toll-like receptor 4 (TLR4) is the key mechanism in Gram-negative bacterial infection-induced sepsis. SAM and HD domain-containing deoxynucleoside triphosphate triphosphohydrolase 1 (SAMHD1) inhibits multiple viruses, but whether it plays a role during bacterial invasion remains unelucidated. Monocyte-macrophage specific Samhd1 knockout (Samhd1−/−) mice and Samhd1−/− macrophage cell line RAW264.7 were constructed and used as research models to evaluate the role of SAMHD1 in TLR4-activated inflammation. In vivo, LPS-challenged Samhd1−/− mice showed higher serum inflammatory factors, accompanied with more severe inflammation infiltration and lower survival rate. In vitro, Samhd1−/− peritoneal macrophages had more activated TLR4 pathway upon LPS-stimulation, accompanied with mitochondrial depolarization and dysfunction and a higher tendency to be M1-polarized. These results could be rescued by overexpressing full-length wild-type SAMHD1 or its phospho-mimetic T634D mutant into Samhd1−/− RAW264.7 cells, whereas the mutants, dNTP hydrolase-function-deprived H238A and phospho-ablative T634A, did not exert the same effect. Lastly, co-IP and immunofluorescence assays confirmed that SAMHD1 interacted with an outer mitochondrial membrane-localized protein, voltage-dependent anion channel-1 (VDAC1). SAMHD1 inhibits TLR4-induced acute inflammation and M1 polarization of macrophages by interacting with VDAC1 and maintaining mitochondria function, which outlines a novel regulatory mechanism of TLR signaling upon LPS stimulation.
Honey’s Yeast—New Source of Valuable Species for Industrial Applications
Authors: Ziuzia Patrycja , Janiec Zuzanna , Wróbel-Kwiatkowska Magdalena , Lazar Zbigniew , Rakicka-Pustułka Magdalena
Published: April 2023

Honey is a rich source of compounds with biological activity; moreover, it is a valuable source of various microorganisms. The aim of this study was to isolate and identify yeast from a sample of lime honey from Poland as well as to assess its ability to biosynthesize value-added chemicals such as kynurenic acid, erythritol, mannitol, and citric acid on common carbon sources. Fifteen yeast strains belonging to the species Yarrowia lipolytica, Candida magnolia, and Starmerella magnoliae were isolated. In shake-flask screening, the best value-added compound producers were chosen. In the last step, scaling up of the culture in the bioreactor was performed. A newly isolated strain of Y. lipolytica No. 12 produced 3.9 mg/L of kynurenic acid growing on fructose. Strain Y. lipolytica No. 9 synthesized 32.6 g/L of erythritol on technical glycerol with a low concentration of byproducts. Strain Y. lipolytica No. 5 produced 15.1 g/L of mannitol on technical glycerol, and strain No. 3 produced a very high amount of citric acid (76.6 g/L) on technical glycerol. In conclusion, to the best of our knowledge this is the first study to report the use of yeast isolates from honey to produce valuable chemicals. This study proves that natural products such as lime honey can be an excellent source of wild-type yeasts with valuable production properties.
Trametinib-Resistant Melanoma Cells Displaying MITFhigh/NGFRlow/IL-8low Phenotype Are Highly Responsive to Alternating Periods of Drug Withdrawal and Drug Rechallenge
Authors: Koziej Paulina , Kluszczynska Katarzyna , Hartman Mariusz L. , Czyz Malgorzata
Published: April 2023

Despite significant advances in targeted therapies against the hyperactivated BRAFV600/MEK pathway for patients with unresectable metastatic melanoma, acquired resistance remains an unsolved clinical problem. In this study, we focused on melanoma cells resistant to trametinib, an agent broadly used in combination therapies. Molecular and cellular changes were assessed during alternating periods of trametinib withdrawal and rechallenge in trametinib-resistant cell lines displaying either a differentiation phenotype (MITFhigh/NGFRlow) or neural crest stem-like dedifferentiation phenotype (NGFRhigh/MITFlow). Neither drug withdrawal nor drug rechallenge induced cell death, and instead of loss of fitness, trametinib-resistant melanoma cells adapted to altered conditions by phenotype switching. In resistant cells displaying a differentiation phenotype, trametinib withdrawal markedly decreased MITF level and activity, which was associated with reduced cell proliferation capacity, and induced stemness assessed as NGFR-positive cells and senescence features, including IL-8 expression and secretion. All these changes could be reversed by trametinib re-exposure, which emphasizes melanoma cell plasticity. Trametinib-resistant cells displaying a dedifferentiation phenotype were less responsive presumably due to the already low level of MITF, a master regulator of the melanoma phenotype. Considering new directions of the development of anti-melanoma treatment, our study suggests that the phenotype of melanomas resistant to targeted therapy might be a crucial determinant of the selection of second-line therapy for melanoma patients.
Combined Structural and Computational Study of the mRubyFT Fluorescent Timer Locked in Its Blue Form
Authors: Boyko Konstantin M. , Khrenova Maria G. , Nikolaeva Alena Y. , Dorovatovskii Pavel V. , Vlaskina Anna V. , Subach Oksana M. , Popov Vladimir O. , Subach Fedor V.
Published: April 2023

The mRubyFT is a monomeric genetically encoded fluorescent timer based on the mRuby2 fluorescent protein, which is characterized by the complete maturation of the blue form with the subsequent conversion to the red one. It has higher brightness in mammalian cells and higher photostability compared with other fluorescent timers. A high-resolution structure is a known characteristic of the mRubyFT with the red form chromophore, but structural details of its blue form remain obscure. In order to obtain insight into this, we obtained an S148I variant of the mRubyFT (mRubyFTS148I) with the blocked over time blue form of the chromophore. X-ray data at a 1.8 Å resolution allowed us to propose a chromophore conformation and its interactions with the neighboring residues. The imidazolidinone moiety of the chromophore is completely matured, being a conjugated π-system. The methine bridge is not oxidized in the blue form bringing flexibility to the phenolic moiety that manifests itself in poor electron density. Integration of these data with the results of molecular dynamic simulation disclosed that the OH group of the phenolic moiety forms a hydrogen bond with the side chain of the T163 residue. A detailed comparison of mRubyFTS148I with other available structures of the blue form of fluorescent proteins, Blue102 and mTagBFP, revealed a number of characteristic differences. Molecular dynamic simulations with the combined quantum mechanic/molecular mechanic potentials demonstrated that the blue form exists in two protonation states, anion and zwitterion, both sharing enolate tautomeric forms of the C=C–O− fragment. These two forms have similar excitation energies, as evaluated by calculations. Finally, excited state molecular dynamic simulations showed that excitation of the chromophore in both protonation states leads to the same anionic fluorescent state. The data obtained shed light on the structural features and spectral properties of the blue form of the mRubyFT timer.
Bact-to-Batch: A Microbiota-Based Tool to Determine Optimal Animal Allocation in Experimental Designs
Authors: Even Gaël , Mouray Anthony , Vandenabeele Nicolas , Martel Sophie , Merlin Sophie , Lebrun-Ruer Ségolène , Chabé Magali , Audebert Christophe
Published: April 2023

The basis of any animal experimentation begins with the housing of animals that should take into account the need for splitting animals into similar groups. Even if it is generally recommended to use the minimum number of animals necessary to obtain reliable and statistically significant results (3Rs rule), the allocation of animals is currently mostly based on randomness. Since variability in gut microbiota is an important confounding factor in animal experiments, the main objective of this study was to develop a new approach based on 16S rRNA gene sequencing analysis of the gut microbiota of animals participating in an experiment, in order to correctly assign the animals across batches. For this purpose, a pilot study was performed on 20 mouse faecal samples with the aim of establishing two groups of 10 mice as similar as possible in terms of their faecal microbiota fingerprinting assuming that this approach limits future analytical bias and ensures reproducibility. The suggested approach was challenged with previously published data from a third-party study. This new method allows to embrace the unavoidable microbiota variability between animals in order to limit artefacts and to provide an additional assurance for the reproducibility of animal experiments.
Amyloid β and Alzheimer’s Disease: Molecular Updates from Physiology to Pathology
Authors: Giuffrida Maria Laura
Published: April 2023

Alzheimer’s disease (AD) represents one of the most challenging disorders, and despite having been widely studied since its first identification, resolutive treatments are still far out of reach [...]
Proteomics Analysis of R-Ras Deficiency in Oxygen Induced Retinopathy
Authors: Vähätupa Maria , Nättinen Janika , Aapola Ulla , Uusitalo-Järvinen Hannele , Uusitalo Hannu , Järvinen Tero A. H.
Published: April 2023

Small GTPase R-Ras regulates vascular permeability in angiogenesis. In the eye, abnormal angiogenesis and hyperpermeability are the leading causes of vision loss in several ischemic retinal diseases such as proliferative diabetic retinopathy (PDR), retinal vein occlusion (RVO), and retinopathy of prematurity (ROP). Oxygen-induced retinopathy (OIR) is the most widely used experimental model for these ischemic retinopathies. To shed more light on how the R-Ras regulates vascular permeability in pathological angiogenesis, we performed a comprehensive (>2900 proteins) characterization of OIR in R-Ras knockout (KO) and wild-type (WT) mice by sequential window acquisition of all theoretical mass spectra (SWATH-MS) proteomics. OIR and age-matched normoxic control retinas were collected at P13, P17, and P42 from R-Ras KO and WT mice and were subjected to SWATH-MS and data analysis. The most significant difference between the R-Ras KO and WT retinas was an accumulation of plasma proteins. The pathological vascular hyperpermeability during OIR in the R-Ras KO retina took place very early, P13. This led to simultaneous hypoxic cell injury/death (ferroptosis), glycolytic metabolism as well compensatory mechanisms to counter the pathological leakage from angiogenic blood vessels in the OIR retina of R-Ras deficient mice.