bluuebunny/crossref_metadata_2025 huggingface dataset:
Photochemical Micro-/Nano-Swelling of Silicone Rubber Induced by Long Pulse-Repetition Interval of an ArF Excimer Laser
Authors: Okoshi Masayuki
Published: May 2021

Long pulse-repetition intervals of 100 to 500 ms of a 193 nm ArF excimer laser successfully increased the height of the photochemical micro-/nano-swelling of silicone rubber, observed with a scanning electron microscope. The effect of the interval was seen despite the heating of the silicone rubber to 80 °C during laser irradiation. The height of the micro-/nano-swelling was saturated when the laser pulse number was 300 or greater, although each of the saturated heights of the micro-/nano-swelling formed by several pulse-repetition intervals was different. Thus, a second ArF excimer laser irradiated the growing micro-/nano-swelling before the saturation; the saturated height of the growing micro-/nano-swelling could be controlled by the pulse-repetition interval of the second ArF excimer laser. To examine the process of micro-/nano-swelling, an early stage of the growth was observed using an atomic force microscope; a dent structure of the micro-/nano-swelling was clearly recognized. In addition, a needle-like structure of the micro-/nano-swelling could be formed when silica glass microspheres were sparsely aligned.
Optimal Identification and Metaheuristic PID Control of a Two-Tank System
Authors: Stefanoiu Dan , Culita Janetta
Published: May 2021

In the modern optimization context, this paper introduces an optimal PID-based control strategy for a two-tank installation, namely ASTANK2. The process model was identified by using raw and spline smoothed measured data, respectively. Two PID controller configurations, a standard (regular) one (PID-R) and a non-standard one (PID-N), were considered for each type of model, resulting in four regulators. The optimal tuning parameters of each regulator were obtained by a searching approach relying on a combination of two metaheuristics. Firstly, an improved version of the Hill Climbing algorithm was employed to comprehensively explore the searching space, aiming to find fairly accurate tuning parameters. Secondly, an improved version of the Firefly Algorithm was proposed to intensively refine the search around the previously found optimal parameters. A comparative analysis between the four controllers was achieved in terms of performance and robustness. The simulation results showed that all optimal controllers yielded good performance in the presence of exogenous stochastic noise (bounded error tracking, setpoint tracking, reduced overshoot, short settling time). Robustness analysis is extensive and illustrates that the PID-R controllers are more robust to model uncertainties, whilst PID-N controllers are more robust to tracking staircase type references.
Assessment of Above-Ground Biomass in Pakistan Forest Ecosystem’s Carbon Pool: A Review
Authors: Khan Ishfaq Ahmad , Khan Waseem Razzaq , Ali Anwar , Nazre Mohd
Published: May 2021

Climate change is acknowledged as a global threat to the environment and human well-being. Forest ecosystems are a significant factor in this regard as they act both as a sink and a source of carbon. Forest carbon evaluation has received more attention after the Paris Agreement. Pakistan has 5.1% forest cover of its total land area, which comprises nine forest types. This study covers the studies conducted on above-ground biomass and carbon stock in various forest types of Pakistan. Most of the studies on biomass and carbon stock estimation have been conducted during 2015–2020. The non-destructive method is mostly followed for carbon stock estimation, followed by remote sensing. The destructive method is used only for developing allometric equations and biomass expansion factors. The information available on the carbon stock and biomass of Pakistan forest types is fragmented and sporadic. Coniferous forests are more important in carbon sequestration and can play a vital role in mitigating climate change. Pakistan is a signatory of the Kyoto Protocol and still lacks regional and national level studies on biomass and carbon stock, which are necessary for reporting under the Kyoto Protocol. This study will help researchers and decision-makers to develop policies regarding Reducing Emissions from Deforestation and forest Degradation (REDD+), conservation, sustainable forest management and enhancement of forest carbon stocks
Change Regularity of Taste and the Performance of Endogenous Proteases in Shrimp (Penaens vannamei) Head during Autolysis
Authors: Wu Shujian , Zhao Mouming , Gao Shijue , Xu Yue , Zhao Xiaoying , Liu Mingyuan , Liu Xiaoling
Published: May 2021

This study evaluated the food safety and proximate composition of shrimp head (SH). Potentially toxic elements in SH were below European Union legislation limits. SH had a high content of tasting amino acids (sweet and umami amino acids was 57%) and a high content of functional amino acids (essential amino acids was 37%). Moreover, the changes of flavor and key umami molecules in SH were studied by sensory evaluation, electronic tongue, electronic nose, automated amino acid analyzer, and high performance liquid chromatography (HPLC). The results showed that the significant difference of flavor in SH happened during autolysis. SH with autolysis had the best umami taste at 6 h, which may result from the synergistic work of free amino acids and nucleotide related compounds. Additionally, the performance of endogenous proteases in SH was investigated to efficiently analyze autolysis. The optimum pH and temperature of endogenous proteases in SH were 7.5 and 50 °C, respectively. The autolysis of SH depends on two endogenous proteases (~50 kDa and ~75 kDa). These results suggest that the formation of flavor in SH during autolysis can be controlled, which could provide guidance for SH recycle. SH could consider as one of the food materials for producing condiments.
Preliminary Study on Tracing the Origin and Exploring the Relations between Growing Conditions and Isotopic and Elemental Fingerprints of Organic and Conventional Cavendish Bananas (Musa spp.)
Authors: Wang Zhijun , Erasmus Sara W. , van Ruth Saskia M.
Published: May 2021

The stable isotopic ratios and elemental compositions of 120 banana samples, Musa spp. (AAA Group, Cavendish Subgroup) cultivar Williams, collected from six countries (Colombia, Costa Rica, Dominica Republic, Ecuador, Panama, Peru), were determined by isotope ratio mass spectrometry and inductively coupled plasma mass spectrometry. Growing conditions like altitude, temperature, rainfall and production system (organic or conventional cultivation) were obtained from the sampling farms. Principal component analysis (PCA) revealed separation of the farms based on geographical origin and production system. The results showed a significant difference in the stable isotopic ratios (δ13C, δ15N, δ18O) and elemental compositions (Al, Ba, Cu, Fe, Mn, Mo, Ni, Rb) of the pulp and peel samples. Furthermore, δ15N was found to be a good marker for organically produced bananas. A correlation analysis was conducted to show the linkage of growing conditions and compositional attributes. The δ13C of pulp and peel were mainly negatively correlated with the rainfall, while δ18O was moderately positively (R values ~0.5) correlated with altitude and temperature. A moderate correlation was also found between temperature and elements such as Ba, Fe, Mn, Ni and Sr in the pulp and peel samples. The PCA results and correlation analysis suggested that the differences of banana compositions were combined effects of geographical factors and production systems. Ultimately, the findings contribute towards understanding the compositional differences of bananas due to different growing conditions and production systems linked to a defined origin; thereby offering a tool to support the traceability of commercial fruits.
Private Value Takeover Auctions with Toeholds: An Experimental Study
Authors: Dodonova Anna , Khoroshilov Yuri
Published: May 2021

This paper presents the results of an experimental study of takeover auctions with toeholds. Consistent with the theory, we find a positive effect of toeholds on bidding. Such an effect, however, is of a lower magnitude and the bidding premium function has an opposite slope than the theory predicts, which can be attributed both to risk aversion and subjects’ tendency to think of their bids in relative terms. Consistent with the theory we find no cross-bidder toehold effect, however, such a result is inconsistent with the observed bidding function if people expect their opponents to behave the same way as they do.
Deterrence by Collective Punishment May Work against Criminals but Never against Freedom Fighters
Authors: Bolle Friedel
Published: May 2021

The main goal of collective punishment (CP) is the deterrence of future “wrong-doing” by freedom fighters or terrorists, protesters against an authoritative government, polluters, students playing pranks on their teacher, football teams lacking enthusiasm, or soldiers showing cowardice to the enemy. CP could consist of the lockout of workers, additional training units for football teams, increased control of athletes and firms, up to the shooting of fellow villagers of assassins. I investigate two classes of problems. In one class, resistance against an authority is individually costly, but enough resistance can be successful (the production of a public good, for example, higher wages after a strike). In the other case, “resistance” is individually profitable (a criminal activity as pollution) and enough “resistance” produces a public bad. We find that, in the first situation, the announcement of CP never decreases the level of resistance. In the second situation, CP can be successful.
Sp1-Mediated circRNA circHipk2 Regulates Myogenesis by Targeting Ribosomal Protein Rpl7
Authors: Yan Junyu , Yang Yalan , Fan Xinhao , Tang Yijie , Tang Zhonglin
Published: May 2021

Circular RNAs (circRNAs) represent a class of covalently closed single-stranded RNA molecules that are emerging as essential regulators of various biological processes. The circRNA circHipk2 originates from exon 2 of the Hipk2 gene in mice and was reported to be involved in acute promyelocytic leukemia and myocardial injury. However, the functions and mechanisms of circHipk2 in myogenesis are largely unknown. Here, to deepen our knowledge about the role of circHipk2, we studied the expression and function of circHipk2 during skeletal myogenesis. We found that circHipk2 was mostly distributed in the cytoplasm, and dynamically and differentially expressed in various myogenesis systems in vitro and in vivo. Functionally, overexpression of circHipk2 inhibited myoblast proliferation and promoted myotube formation in C2C12 cells, whereas the opposite effects were observed after circHipk2 knockdown. Mechanistically, circHipk2 could directly bind to ribosomal protein Rpl7, an essential 60S preribosomal assembly factor, to inhibit ribosome translation. In addition, we verified that transcription factor Sp1 directly bound to the promoter of circHipk2 and affected the expression of Hipk2 and circHipk2 in C2C12 myoblasts. Collectively, these findings identify circHipk2 as a candidate circRNA regulating ribosome biogenesis and myogenesis proliferation and differentiation.
Versatile Physiological Functions of Plant GSK3-Like Kinases
Authors: Mao Juan , Li Wenxin , Liu Jing , Li Jianming
Published: May 2021

The plant glycogen synthase kinase 3 (GSK3)-like kinases are highly conserved protein serine/threonine kinases that are grouped into four subfamilies. Similar to their mammalian homologs, these kinases are constitutively active under normal growth conditions but become inactivated in response to diverse developmental and environmental signals. Since their initial discoveries in the early 1990s, many biochemical and genetic studies were performed to investigate their physiological functions in various plant species. These studies have demonstrated that the plant GSK3-like kinases are multifunctional kinases involved not only in a wide variety of plant growth and developmental processes but also in diverse plant stress responses. Here we summarize our current understanding of the versatile physiological functions of the plant GSK3-like kinases along with their confirmed and potential substrates.
Changes in Motor Competence after a Brief Physical Education Intervention Program in 4 and 5-Year-Old Preschool Children
Authors: Navarro-Patón Rubén , Brito-Ballester Julien , Villa Silvia Pueyo , Anaya Vanessa , Mecías-Calvo Marcos
Published: May 2021

Low motor competence (MC) can cause low participation in physical activities in preschool children, and together with a high caloric intake, it can lead to obesity. Interventions on motor skills are effective in the short term to improve MC, therefore the objectives of this study were (1) to investigate the effect of a short six-week program on levels of motor competence in preschool children, and (2) to examine the effects of gender-based intervention. A total of 156 preschool children (5.20 ± 0.54 years old) from Lugo (Spain) participated. A quasi-experimental pre–post-test design was used with a control group of 76 students. The Movement Assessment Battery for Children—2nd Edition (MABC-2) was used to collect the data. Significant differences between the control and experimental groups were found after the intervention program in aiming and catching (p < 0.001), balance (p < 0.001), the total score of eight tests (p < 0.001), and total percentile score (p < 0.001). The results regarding gender in the experimental group showed a reduction in differences with respect to the initial results except in aiming and catching, where scores were higher in boys. The data suggest that the application of specific intervention programs in MC could positively influence the improvement of MC in preschool children, thus reducing differences between genders.
Developing and Evaluating a Continuous Education Program for Healthcare Assistants in Macao: A Cluster-Randomized Trial
Authors: Cheong Pak-Leng , Hsu Nanly
Published: May 2021

The shortage of healthcare human resources is an important challenge for coping with the aging society in Macao. Since little attention has been paid to continuous education of healthcare assistants, this study aims to develop and evaluate a continuous education program, supporting the expansion and optimization of the competence of healthcare assistants. It is a cluster-randomized trial study. All healthcare assistants who were employed in nursing homes in Macao were eligible for this study. Six nursing homes were recruited and randomly assigned either an experimental group (3 nursing homes; 45 healthcare assistants) or a control group (3 nursing homes; 40 healthcare assistants). Healthcare assistants were assessed at baseline and after intervention with the Healthcare Assistants Care Knowledge Test and the Healthcare Assistants Care Competence Self-Assessment. The experimental group received a continuous education program with 10 themes during 2017–2018 while the control groups did not. The results of the generalized estimating equation showed that care knowledge in the experimental group was significantly different from that of the control group (Wald Chi Square = 3.848, p < 0.05) as well as care competence (Wald Chi Square = 13.361, p < 0.001). This study developed a continuous program for health assistants and provided evidence that continuous education programs improve and maintain the level of care knowledge and care competency of healthcare assistants.
Alcohol in Psoriasis—From Bench to Bedside
Authors: Szentkereszty-Kovács Zita , Gáspár Krisztián , Szegedi Andrea , Kemény Lajos , Kovács Dóra , Törőcsik Dániel
Published: May 2021

Alcohol affects the symptoms, compliance and comorbidities as well as the safety and efficacy of treatments in psoriatic patients. In this review, we aim to summarize and link clinical observations with a molecular background, such as signaling pathways at the cellular level and genetic variations, and to provide an overview of how this knowledge could influence our treatment selection and patient management.
Border Habitat Effects on Captures of Halyomorpha halys (Hemiptera: Pentatomidae) in Pheromone Traps and Fruit Injury at Harvest in Apple and Peach Orchards in the Mid-Atlantic, USA
Authors: Bergh James Christopher , Morrison William R. , Stallrich Jon W. , Short Brent D. , Cullum John P. , Leskey Tracy C.
Published: May 2021

The invasive Halyomorpha halys invades crop fields from various bordering habitats, and its feeding on crops has caused significant economic losses. Thus, H. halys is considered a perimeter-driven threat, and research on alternative management tactics against it has focused on intervention at crop edges. Woodlands adjacent to crop fields contain many hosts of H. halys and are therefore considered “riskiest” in terms of pest pressure and crop injury. However, tree fruit orchards in the Mid-Atlantic, USA, are often bordered on one or more sides by woodlands and other habitats, including other tree fruit blocks, and field crops. Monitoring H. halys using pheromone traps has most often focused on the crop–woodland interface, but the relative effects of woodlands and other habitats bordering orchards on pest pressure and crop injury have not been examined. A two-year study comparing seasonal captures of H. halys and fruit injury among different habitats bordering commercial apple and peach orchards in the Mid-Atlantic revealed that while woodland borders often posed the greatest risk, other border habitats also contributed significantly to captures and injury in numerous instances. The relevance of these findings to refining and optimizing perimeter-based monitoring and management approaches for H. halys is discussed.
Potential Use of Indoor Living Walls in Canadian Dwellings
Authors: Smolova Daria , Friedman Avi
Published: May 2021

Current social and environmental challenges have led to the rethinking of residential designs. Global warming, food insecurity, and, as a result, costly fresh produce are some of the causes of the reconsideration. Moreover, with obligatory isolation following the global COVID-19 pandemic, some are realizing the importance of nature and air quality in homes. This paper explores the potential integration of indoor living walls (ILWs) in Canadian homes for agricultural and air purification purposes. By reviewing a number of case studies, this paper investigates how the development of such walls can alter the traditional food production chain, while reducing environmental threats. The findings show that current indoor living wall practices can be transformed into a useful source of fresh food, and, to some degree, alter traditional food supply. They can also help in creating inexpensive methods of air purification.
Biological and Clinical Significance of Mosaicism in Human Preimplantation Embryos
Authors: Bouba Ioanna , Hatzi Elissavet , Ladias Paris , Sakaloglou Prodromos , Kostoulas Charilaos , Georgiou Ioannis
Published: May 2021

Applications and indications of assisted reproduction technology are expanding, but every new approach is under scrutiny and thorough consideration. Recently, groups of assisted reproduction experts have presented data that support the clinical use of mosaic preimplantation embryos at the blastocyst stage, previously excluded from transfer. In the light of published contemporary studies, with or without clinical outcomes, there is growing evidence that mosaic embryos have the capacity for further in utero development and live birth. Our in-depth discussion will enable readers to better comprehend current developments. This expansion into the spectrum of ART practices requires further evidence and further theoretical documentation, basic research, and ethical support. Therefore, if strict criteria for selecting competent mosaic preimplantation embryos for further transfer, implantation, fetal growth, and healthy birth are applied, fewer embryos will be excluded, and more live births will be achieved. Our review aims to discuss the recent literature on the transfer of mosaic preimplantation embryos. It also highlights controversies as far as the clinical utilization of preimplantation embryos concerns. Finally, it provides the appropriate background to elucidate and highlight cellular and genetic aspects of this novel direction.
E-Cadherin/HMR-1 Membrane Enrichment Is Polarized by WAVE-Dependent Branched Actin
Authors: Cordova-Burgos Luigy , Patel Falshruti B. , Soto Martha C.
Published: May 2021

Polarized epithelial cells adhere to each other at apical junctions that connect to the apical F-actin belt. Regulated remodeling of apical junctions supports morphogenesis, while dysregulated remodeling promotes diseases such as cancer. We have documented that branched actin regulator, WAVE, and apical junction protein, Cadherin, assemble together in developing C. elegans embryonic junctions. If WAVE is missing in embryonic epithelia, too much Cadherin assembles at apical membranes, and yet apical F-actin is reduced, suggesting the excess Cadherin is not fully functional. We proposed that WAVE supports apical junctions by regulating the dynamic accumulation of Cadherin at membranes. To test this model, here we examine if WAVE is required for Cadherin membrane enrichment and apical–basal polarity in a maturing epithelium, the post-embryonic C. elegans intestine. We find that larval and adult intestines have distinct apicobasal populations of Cadherin, each with distinct dependence on WAVE branched actin. In vivo imaging shows that loss of WAVE components alters post-embryonic E-cadherin membrane enrichment, especially at apicolateral regions, and alters the lateral membrane. Analysis of a biosensor for PI(4,5)P2 suggests loss of WAVE or Cadherin alters the polarity of the epithelial membrane. EM (electron microscopy) illustrates lateral membrane changes including separations. These findings have implications for understanding how mutations in WAVE and Cadherin may alter cell polarity.
Biological Determinants of Track and Field Throwing Performance
Authors: Zaras Nikolaos , Stasinaki Angeliki-Nikoletta , Terzis Gerasimos
Published: May 2021

Track and field throwing performance is determined by a number of biomechanical and biological factors which are affected by long-term training. Although much of the research has focused on the role of biomechanical factors on track and field throwing performance, only a small body of scientific literature has focused on the connection of biological factors with competitive track and field throwing performance. The aim of this review was to accumulate and present the current literature connecting the performance in track and field throwing events with specific biological factors, including the anthropometric characteristics, the body composition, the neural activation, the fiber type composition and the muscle architecture characteristics. While there is little published information to develop statistical results, the results from the current review suggest that major biological determinants of track and field throwing performance are the size of lean body mass, the neural activation of the protagonist muscles during the throw and the percentage of type II muscle fiber cross-sectional area. Long-term training may enhance these biological factors and possibly lead to a higher track and field throwing performance. Consequently, coaches and athletes should aim at monitoring and enhancing these parameters in order to increase track and field throwing performance.
Emotion Recognition from Realistic Dynamic Emotional Expressions Cohere with Established Emotion Recognition Tests: A Proof-of-Concept Validation of the Emotional Accuracy Test
Authors: Israelashvili Jacob , Pauw Lisanne S. , Sauter Disa A. , Fischer Agneta H.
Published: May 2021

Individual differences in understanding other people’s emotions have typically been studied with recognition tests using prototypical emotional expressions. These tests have been criticized for the use of posed, prototypical displays, raising the question of whether such tests tell us anything about the ability to understand spontaneous, non-prototypical emotional expressions. Here, we employ the Emotional Accuracy Test (EAT), which uses natural emotional expressions and defines the recognition as the match between the emotion ratings of a target and a perceiver. In two preregistered studies (Ntotal = 231), we compared the performance on the EAT with two well-established tests of emotion recognition ability: the Geneva Emotion Recognition Test (GERT) and the Reading the Mind in the Eyes Test (RMET). We found significant overlap (r > 0.20) between individuals’ performance in recognizing spontaneous emotions in naturalistic settings (EAT) and posed (or enacted) non-verbal measures of emotion recognition (GERT, RMET), even when controlling for individual differences in verbal IQ. On average, however, participants reported enjoying the EAT more than the other tasks. Thus, the current research provides a proof-of-concept validation of the EAT as a useful measure for testing the understanding of others’ emotions, a crucial feature of emotional intelligence. Further, our findings indicate that emotion recognition tests using prototypical expressions are valid proxies for measuring the understanding of others’ emotions in more realistic everyday contexts.
Potential of Intestinal Current Measurement for Personalized Treatment of Patients with Cystic Fibrosis
Authors: Graeber Simon Y. , Vitzthum Constanze , Mall Marcus A.
Published: May 2021

Refinement of personalized treatment of cystic fibrosis (CF) with emerging medicines targeting the CF basic defect will likely benefit from biomarkers sensitive to detect improvement of cystic fibrosis transmembrane conductance regulator (CFTR) function in individual patients. Intestinal current measurement (ICM) is a technique that enables quantitative assessment of CFTR chloride channel function in rectal tissues or other intestinal epithelia. ICM was originally developed to study the CF ion transport defect in the intestine and has been established as a sensitive biomarker of CFTR function and diagnostic test for CF. With the emergence of CFTR-directed therapeutics, ICM has become an important tool to estimate the level of rescue of CFTR function achieved by approved CFTR modulators, both at the level of CFTR genotype groups, as well as individual patients with CF. In combination with preclinical patient-derived cell culture models, ICM may aid the development of targeted therapies for patients with rare CFTR mutations. Here, we review the principles of ICM and examine how this CFTR biomarker may be used to support diagnostic testing and enhance personalized medicine for individual patients with common as well as rare CFTR mutations in the new era of medicines targeting the underlying cause of CF.
Effect of Cathodic Protection on Reinforced Concrete with Fly Ash Using Electrochemical Noise
Authors: García-Contreras Jorge , Gaona-Tiburcio Citlalli , López-Cazares Irene , Sanchéz-Díaz Guillermo , Ibarra Castillo Juan Carlos , Jáquez-Muñoz Jesús , Nieves-Mendoza Demetrio , Maldonado-Bandala Erick , Olguín-Coca Javier , López-León Luis Daimir , Almeraya-Calderón Facundo
Published: May 2021

Corrosion of steel reinforcement is the major factor that limits the durability and serviceability performance of reinforced concrete structures. Impressed current cathodic protection (ICCP) is a widely used method to protect steel reinforcements against corrosion. This research aimed to study the effect of cathodic protection on reinforced concrete with fly ash using electrochemical noise (EN). Two types of reinforced concrete mixtures were manufactured; 100% Ordinary Portland Cement (OCP) and replacing 15% of cement using fly ash (OCPFA). The specimens were under-designed protected conditions (−1000 ≤ E ≤ −850 mV vs. Ag/AgCl) and cathodic overprotection (E < −1000 mV vs. Ag/AgCl) by impressed current, and specimens concrete were immersed in a 3.5 wt.% sodium chloride (NaCl) Solution. The analysis of electrochemical noise-time series showed that the mixtures microstructure influenced the corrosion process. Transients of uniform corrosion were observed in the specimens elaborated with (OPC), unlike those elaborated with (OPCFA). This phenomenon marked the difference in the concrete matrix’s hydration products, preventing Cl− ions flow and showing passive current and potential transients in most specimens.
A Review: Halogenated Compounds from Marine Actinomycetes
Authors: Wang Cong , Du Weisheng , Lu Huanyun , Lan Jianzhou , Liang Kailin , Cao Shugeng
Published: May 2021

Marine actinomycetes, Streptomyces species, produce a variety of halogenated compounds with diverse structures and a range of biological activities owing to their unique metabolic pathways. These halogenated compounds could be classified as polyketides, alkaloids (nitrogen-containing compounds) and terpenoids. Halogenated compounds from marine actinomycetes possess important biological properties such as antibacterial and anticancer activities. This review reports the sources, chemical structures and biological activities of 127 new halogenated compounds originated mainly from Streptomyces reported from 1992 to 2020.
Recommended Tests for the Self-Disproportionation of Enantiomers (SDE) to Ensure Accurate Reporting of the Stereochemical Outcome of Enantioselective Reactions
Authors: Han Jianlin , Wzorek Alicja , Klika Karel D. , Soloshonok Vadim A.
Published: May 2021

The purpose of this review is to highlight the necessity of conducting tests to gauge the magnitude of the self-disproportionation of enantiomers (SDE) phenomenon to ensure the veracity of reported enantiomeric excess (ee) values for scalemic samples obtained from enantioselective reactions, natural products isolation, etc. The SDE always occurs to some degree whenever any scalemic sample is subjected to physicochemical processes concomitant with the fractionation of the sample, thus leading to erroneous reporting of the true ee of the sample if due care is not taken to either preclude the effects of the SDE by measurement of the ee prior to the application of physicochemical processes, suppressing the SDE, or evaluating all obtained fractions of the sample. Or even avoiding fractionation altogether if possible. There is a clear necessity to conduct tests to assess the magnitude of the SDE for the processes applied to samples and the updated and improved recommendations described herein cover chromatography and processes involving gas-phase transformations such as evaporation or sublimation.
Onion (Allium cepa L.)-Derived Nanoparticles Inhibited LPS-Induced Nitrate Production, However, Their Intracellular Incorporation by Endocytosis Was Not Involved in This Effect on RAW264 Cells
Authors: Yamasaki Masao , Yamasaki Yumi , Furusho Rina , Kimura Hayaka , Kamei Ichiro , Sonoda Hiroko , Ikeda Masahiro , Oshima Tatsuya , Ogawa Kenjiro , Nishiyama Kazuo
Published: May 2021

The aim of this study was to evaluate the involvement of nanoparticles prepared from Allium cepa L. as anti-inflammatory agents. In the present study, we identified nanoparticles from Allium cepa L. using the ultracentrifugation exosome purification method. The nanoparticles were referred to as 17,000× g and 200,000× g precipitates, and they contained quercetins, proteins, lipids, and small-sized RNA. The nanoparticles inhibited nitric oxide production from lipopolysaccharide (LPS)-stimulated RAW264 cells without cytotoxic properties. Cellular incorporation was confirmed by laser microscopic observation after PKH26 staining. The inhibition of caveolae-dependent endocytosis and macropinocytosis significantly prevented the incorporation of the nanoparticles but had no effect on the inhibition of nitric oxide in RAW264 cells. Collectively, the identified nanoparticles were capable of inhibiting the LPS response via extracellular mechanisms. Taken together, the way of consuming Allium cepa L. without collapsing the nanoparticles is expected to provide an efficient anti-inflammatory effect.
Control of Structural and Magnetic Properties of Polycrystalline Co2FeGe Films via Deposition and Annealing Temperatures
Authors: Vovk Andrii , Bunyaev Sergey A. , Štrichovanec Pavel , Vovk Nikolay R. , Postolnyi Bogdan , Apolinario Arlete , Pardo José Ángel , Algarabel Pedro Antonio , Kakazei Gleb N. , Araujo João Pedro
Published: May 2021

Thin polycrystalline Co2FeGe films with composition close to stoichiometry have been fabricated using magnetron co-sputtering technique. Effects of substrate temperature (TS) and post-deposition annealing (Ta) on structure, static and dynamic magnetic properties were systematically studied. It is shown that elevated TS (Ta) promote formation of ordered L21 crystal structure. Variation of TS (Ta) allow modification of magnetic properties in a broad range. Saturation magnetization ~920 emu/cm3 and low magnetization damping parameter α ~ 0.004 were achieved for TS = 573 K. This in combination with soft ferromagnetic properties (coercivity below 6 Oe) makes the films attractive candidates for spin-transfer torque and magnonic devices.
Optical Constants of Chemical Vapor Deposited Graphene for Photonic Applications
Authors: El-Sayed Marwa A. , Ermolaev Georgy A. , Voronin Kirill V. , Romanov Roman I. , Tselikov Gleb I. , Yakubovsky Dmitry I. , Doroshina Natalia V. , Nemtsov Anton B. , Solovey Valentin R. , Voronov Artem A. , Novikov Sergey M. , Vyshnevyy Andrey A. , Markeev Andrey M. , Arsenin Aleksey V. , Volkov Valentyn S.
Published: May 2021

Graphene is a promising building block material for developing novel photonic and optoelectronic devices. Here, we report a comprehensive experimental study of chemical-vapor deposited (CVD) monolayer graphene’s optical properties on three different substrates for ultraviolet, visible, and near-infrared spectral ranges (from 240 to 1000 nm). Importantly, our ellipsometric measurements are free from the assumptions of additional nanometer-thick layers of water or other media. This issue is critical for practical applications since otherwise, these additional layers must be included in the design models of various graphene photonic, plasmonic, and optoelectronic devices. We observe a slight difference (not exceeding 5%) in the optical constants of graphene on different substrates. Further, the optical constants reported here are very close to those of graphite, which hints on their applicability to multilayer graphene structures. This work provides reliable data on monolayer graphene’s optical properties, which should be useful for modeling and designing photonic devices with graphene.
Enhanced Light Absorption by Facile Patterning of Nano-Grating on Mesoporous TiO2 Photoelectrode for Cesium Lead Halide Perovskite Solar Cells
Authors: Kim Kang-Pil , Kim Wook , Kwon Soo , Kim Jun , Do Yun , Woo Sungho
Published: May 2021

CsPbIBr2, a cesium-based all-inorganic halide perovskite (CsPe), is a very promising alternative material to mainstream organic–inorganic hybrid halide perovskite (HPe) materials owing to its exceptional moisture stability, thermal stability, and light stability. However, because of the wide band gap (2.05 eV) of CsPbIBr2, it has a low power conversion efficiency (PCE), which hinders its application in highly efficient solar cells. In this study, a facile nanoimprinted one-dimensional grating nanopattern (1D GNP) formation on mesoporous TiO2 (mp-TiO2) photoelectrodes was introduced to improve the effective light utilization and enhance the performance of CsPbIBr2 perovskite solar cells (PSCs). The 1D GNP structure on the mp-TiO2 layer increases the light absorption efficiency by diffracting the unabsorbed light into the active mp-TiO2 and CsPbIBr2 layers as well as increasing the charge separation and collection due to the extended interfacial contact area between the mp-TiO2 and CsPbIBr2 layers. Consequently, both the current density (JSC) and the fill factor (FF) of the fabricated cells improved, leading to over a 20% enhancement in the solar cell’s PCE. Thus, this periodic grating structure, fabricated by simple nanoimprinting, could play an important role in the large-scale production of highly efficient and cost-effective Cs-based PSCs.
Biocompatibility of Bacterial Magnetosomes as MRI Contrast Agent: A Long-Term In Vivo Follow-Up Study
Authors: Nan Xiaohui , Lai Wenjia , Li Dan , Tian Jiesheng , Hu Zhiyuan , Fang Qiaojun
Published: May 2021

Derived from magnetotactic bacteria (MTB), magnetosomes consist of magnetite crystals enclosed within a lipid bilayer membrane and are known to possess advantages over artificially synthesized nanoparticles because of the narrow size distribution, uniform morphology, high purity and crystallinity, single magnetic domain, good biocompatibility, and easy surface modification. These unique properties have increasingly attracted researchers to apply bacterial magnetosomes (BMs) in the fields of biology and medicine as MRI imaging contrast agents. Due to the concern of biosafety, a long-term follow-up of the distribution and clearance of BMs after entering the body is necessary. In this study, we tracked changes of BMs in major organs of mice up to 135 days after intravenous injection using a combination of several techniques. We not only confirmed the liver as the well-known targeted organs of BMs, but also found that BMs accumulated in the spleen. Besides, two major elimination paths, as well as the approximate length of time for BMs to be cleared from the mice, were revealed. Together, the results not only confirm that BMs have high biocompatibility, but also provide a long-term in-vivo assessment which may further help to forward the clinical applications of BMs as an MRI contrast agent.
Characteristics and Electronic Band Alignment of a Transparent p-CuI/n-SiZnSnO Heterojunction Diode with a High Rectification Ratio
Authors: Lee Jeong Hyuk , Lee Byeong Hyeon , Kang Jeonghun , Diware Mangesh , Jeon Kiseok , Jeong Chaehwan , Lee Sang Yeol , Kim Kee Hoon
Published: May 2021

Transparent p-CuI/n-SiZnSnO (SZTO) heterojunction diodes are successfully fabricated by thermal evaporation of a (111) oriented p-CuI polycrystalline film on top of an amorphous n-SZTO film grown by the RF magnetron sputtering method. A nitrogen annealing process reduces ionized impurity scattering dominantly incurred by Cu vacancy and structural defects at the grain boundaries in the CuI film to result in improved diode performance; the current rectification ratio estimated at ±2 V is enhanced from ≈106 to ≈107. Various diode parameters, including ideality factor, reverse saturation current, offset current, series resistance, and parallel resistance, are estimated based on the Shockley diode equation. An energy band diagram exhibiting the type-II band alignment is proposed to explain the diode characteristics. The present p-CuI/n-SZTO diode can be a promising building block for constructing useful optoelectronic components such as a light-emitting diode and a UV photodetector.
Emerging Data on the Diversity of Molecular Mechanisms Involving C/D snoRNAs
Authors: Baldini Laeya , Charpentier Bruno , Labialle Stéphane
Published: May 2021

Box C/D small nucleolar RNAs (C/D snoRNAs) represent an ancient family of small non-coding RNAs that are classically viewed as housekeeping guides for the 2′-O-methylation of ribosomal RNA in Archaea and Eukaryotes. However, an extensive set of studies now argues that they are involved in mechanisms that go well beyond this function. Here, we present these pieces of evidence in light of the current comprehension of the molecular mechanisms that control C/D snoRNA expression and function. From this inventory emerges that an accurate description of these activities at a molecular level is required to let the snoRNA field enter in a second age of maturity.
The TOTUM-63 Supplement and High-Intensity Interval Training Combination Limits Weight Gain, Improves Glycemic Control, and Influences the Composition of Gut Mucosa-Associated Bacteria in Rats on a High Fat Diet
Authors: Dupuit Marine , Chavanelle Vivien , Chassaing Benoit , Perriere Fanny , Etienne Monique , Plissonneau Claire , Boscaro Audrey , Barnich Nicolas , Pialoux Vincent , Maugard Thierry , Le Joubioux Florian , Peltier Sébastien , Sirvent Pascal , Otero Yolanda F. , Boisseau Nathalie
Published: May 2021

Obesity and prediabetes are the two strongest risk factors of type 2 diabetes. It has been reported that TOTUM-63, a polyphenol-rich plant extract, has beneficial effects on body weight (BW) and insulin resistance in mice fed a high fat diet (HFD). The study aim was to determine whether high-intensity interval training (HIIT) and/or TOTUM-63 supplementation improved body composition and glycemic control and gut microbiota composition in a Western diet-induced obesity rat model. Wistar rats received a standard diet (CTRL; control; n = 12) or HFD (HFD; n = 48) for 16 weeks. Then, HFD rats were divided in four groups: HFD, HFD + TOTUM-63 (T63), HFD + HIIT (HIIT), and HFD + HIIT +T63 (HIIT + T63). Training was performed 4 days/week for 12 weeks. TOTUM-63 was included in diet composition (2%). The HIIT + T63 combination significantly limited BW gain, without any energy intake modulation, and improved glycemic control. BW variation was correlated with increased α-diversity of the colon mucosa microbiota in the HIIT + T63 group. Moreover, the relative abundance of Anaeroplasma, Christensenellaceae and Oscillospira was higher in the HIIT + T63 group. Altogether, these results suggest that the HIIT and TOTUM-63 combination could be proposed for the management of obesity and prediabetes.
Fasting: How to Guide
Authors: Attinà Alda , Leggeri Claudia , Paroni Rita , Pivari Francesca , Dei Cas Michele , Mingione Alessandra , Dri Maria , Marchetti Marco , Di Renzo Laura
Published: May 2021

Fasting potentials are the most interesting topics in the Nutritional Era. Fasting consists of the catabolism of lipids, proteins, and carbohydrates to maintain blood glucose levels in a normal range. The action mechanisms of fasting were firstly understood in minor organisms and later in humans. Nutritional interventions of caloric restriction could attenuate age-associated epigenetic alterations and could have a protective effect against cellular alterations, promoting longevity and health span. While most fasting studies point out the weight and fat mass decreases, it is important to define specific guidelines for fasting and non-fasting days to enhance adherence, minimize the dropout rates of the interventions, and maximize body composition improvement. Although the panorama of evidence on fasting and caloric restriction is wide, there is a lack of a safe fasting protocol to guide physicians in its prescription. The main goal is to identify a how to use guide, a major posology of fasting, inserted within a huge dietetic personalized strategy leading to an optimal and healthy nutritional status.
The Promotions of Sustainable Lunch Meals in School Feeding Programs: The Case of Italy
Authors: Rossi Laura , Ferrari Marika , Martone Deborah , Benvenuti Luca , De Santis Alberto
Published: May 2021

School is considered a privileged environment for health education and school feeding represents an opportunity for promoting sustainable foods to young generations. The objective of this paper is to demonstrate that is possible to select, from existing school menus, recipes that combine healthy foods with low environmental impact. A national sample of Italian school menus was collected and a total number of 194 recipes were included on a database containing 70 first courses, 83 s courses, 39 side dishes, 1 portion of fruit, and 1 portion of bread. A mathematical model was conceived to combine nutritional adequacy and acceptability criteria while minimizing GHGs emissions. The result is a four-week menu characterized by large vegetable components that were used not only as side dishes but also as ingredients in the first and second courses. Legumes and pasta are often included, and white meat is selected instead of red meat. The findings presented in this paper demonstrated that it is possible to design environmental-friendly meals from existing school menus. The mathematical model developed in this work has the potentiality of being completely scalable, easily updatable, and widely utilizable in different settings either for design or monitoring purposes as well as for research data collection.
Variation of Glucosinolate Contents in Clubroot-Resistant and -Susceptible Brassica napus Cultivars in Response to Virulence of Plasmodiophora brassicae
Authors: Zamani-Noor Nazanin , Hornbacher Johann , Comel Christel Joy , Papenbrock Jutta
Published: May 2021

The present study investigated the changes in total and individual glucosinolates (GSLs) in roots and leaves of different clubroot-resistant and -susceptible oilseed rape cultivars following artificial inoculation with Plasmodiophora brassicae isolates with different virulence. The results showed significant differences in clubroot incidence and severity as well as in the amount of total and individual glucosinolates between oilseed rape cultivars in response to virulence of the pathogen. Single among with total aliphatic and total indolic glucosinolate contents were significantly lower in leaves of susceptible cultivars compared to resistant ones due to the infection. Similarly, single and total aliphatic as well as indolic glucosinolate contents in roots were lower in susceptible cultivars compared to resistant cultivars analyzed. The different isolates of P. brassicae seem to differ in their ability to reduce gluconasturtiin contents in the host. The more aggressive isolate P1 (+) might be able to suppress gluconasturtiin synthesis of the host in a more pronounced manner compared to the isolate P1. A possible interaction of breakdown products of glucobrassicin with the auxin receptor transport inhibitor response 1 (TIR1) is hypothesized and its possible effects on auxin signaling in roots and leaves of resistant and susceptible cultivars is discussed. A potential interplay between aliphatic and indolic glucosinolates that might be involved in water homeostasis in resistant cultivars is explained.