bluuebunny/crossref_metadata_2025 huggingface dataset:
Serum Interleukins 8, 17, and 33 as Potential Biomarkers of Colon Cancer
Authors: Tâlvan Constantin-Dan , Budișan Liviuța , Tâlvan Elena-Teodora , Grecu Valentin , Zănoagă Oana , Mihalache Cosmin , Cristea Victor , Berindan-Neagoe Ioana , Mohor Călin Ilie
Published: February 2024

This research investigated the serum levels of three interleukins (IL8, IL17A, and IL33) and the possible relationships between them in healthy people and colon cancer patients at different stages. This study involved 82 participants, 42 of whom had colon cancer and 40 were healthy individuals. The cancer patients were classified into four groups according to the TNM staging classification of colon and rectal cancer. Serum levels of the interleukins were measured by the ELISA test. The data were analyzed statistically to compare the demographic characteristics, the interleukin levels across cancer stages, and the correlation between interleukins in both groups. The results showed that women had more early-stage colon cancer diagnoses, while men had more advanced-stage cancer diagnoses. Stage two colon cancer was more common in older people. Younger people, men, and those with early-stage colon cancer had higher levels of interleukins. The levels of IL8 and IL17A were higher in the cancer group, while the level of IL33 was higher in the healthy group. There was a strong correlation between IL8 and IL17A levels in both groups (p = 0.001). IL17A influenced the level of IL33 in the cancer group (p = 0.007). This study suggested that cytokine variation profiles could be useful for detecting colon cancer and predicting its outcome.
Roles of Androgen Receptor Signaling in Urothelial Carcinoma
Authors: Sundi Debasish , Collier Katharine A. , Yang Yuanquan , Diaz Dayssy Alexandra , Pohar Kamal S. , Singer Eric A. , Gupta Sanjay , Carson William E. , Clinton Steven K. , Li Zihai , Messing Edward M.
Published: February 2024

Preclinical and clinical data suggest that androgen receptor signaling strongly contributes to bladder cancer development. The roles of the androgen receptor in bladder carcinogenesis have obvious implications for understanding the strong male sex bias in this disease and for potential therapeutic strategies as well. In this review, we summarize what is known about androgen receptor signaling in urothelial carcinoma as well as in tumor-infiltrating immune cells, reviewing preclinical and clinical data. We also highlight clinical trial efforts in this area.
PathoGraph: An Attention-Based Graph Neural Network Capable of Prognostication Based on CD276 Labelling of Malignant Glioma Cells
Authors: Alzoubi Islam , Zhang Lin , Zheng Yuqi , Loh Christina , Wang Xiuying , Graeber Manuel B.
Published: February 2024

Computerized methods have been developed that allow quantitative morphological analyses of whole slide images (WSIs), e.g., of immunohistochemical stains. The latter are attractive because they can provide high-resolution data on the distribution of proteins in tissue. However, many immunohistochemical results are complex because the protein of interest occurs in multiple locations (in different cells and also extracellularly). We have recently established an artificial intelligence framework, PathoFusion which utilises a bifocal convolutional neural network (BCNN) model for detecting and counting arbitrarily definable morphological structures. We have now complemented this model by adding an attention-based graph neural network (abGCN) for the advanced analysis and automated interpretation of such data. Classical convolutional neural network (CNN) models suffer from limitations when handling global information. In contrast, our abGCN is capable of creating a graph representation of cellular detail from entire WSIs. This abGCN method combines attention learning with visualisation techniques that pinpoint the location of informative cells and highlight cell–cell interactions. We have analysed cellular labelling for CD276, a protein of great interest in cancer immunology and a potential marker of malignant glioma cells/putative glioma stem cells (GSCs). We are especially interested in the relationship between CD276 expression and prognosis. The graphs permit predicting individual patient survival on the basis of GSC community features. Our experiments lay a foundation for the use of the BCNN-abGCN tool chain in automated diagnostic prognostication using immunohistochemically labelled histological slides, but the method is essentially generic and potentially a widely usable tool in medical research and AI based healthcare applications.
The Contemporary Role of Hematopoietic Stem Cell Transplantation in the Management of Chronic Myeloid Leukemia: Is It the Same in All Settings?
Authors: Elmakaty Ibrahim , Saglio Giuseppe , Al-Khabori Murtadha , Elsayed Abdelrahman , Elsayed Basant , Elmarasi Mohamed , Elsabagh Ahmed Adel , Alshurafa Awni , Ali Elrazi , Yassin Mohamed
Published: February 2024

Hematopoietic stem cell transplantation (HSCT) for chronic myeloid leukemia (CML) patients has transitioned from the standard of care to a treatment option limited to those with unsatisfactory tyrosine kinase inhibitor (TKI) responses and advanced disease stages. In recent years, the threshold for undergoing HSCT has increased. Most CML patients now have life expectancies comparable to the general population, and therefore, the goal of therapy is shifting toward achieving treatment-free remission (TFR). While TKI discontinuation trials in CML show potential for achieving TFR, relapse risk is high, affirming allogeneic HSCT as the sole curative treatment. HSCT should be incorporated into treatment algorithms from the time of diagnosis and, in some patients, evaluated as soon as possible. In this review, we will look at some of the recent advances in HSCT, as well as its indication in the era of aiming for TFR in the presence of TKIs in CML.
Interleukin-6 as a Predictive Factor of Pathological Response to FLOT Regimen Systemic Treatment in Locally Advanced Gastroesophageal Junction or Gastric Cancer Patients
Authors: Marcisz-Grzanka Katarzyna , Kotowicz Beata , Nowak Aleksandra , Winiarek Mariola , Fuksiewicz Malgorzata , Kowalska Maria , Tysarowski Andrzej , Olesinski Tomasz , Palucki Jakub , Sulkowska Urszula , Kolasinska-Cwikla Agnieszka , Wyrwicz Lucjan Stanislaw
Published: February 2024

Background: Perioperative treatment is a gold standard in locally advanced gastric cancer or GEJ cancer in the Western population. Unfortunately, the response rate after neoadjuvant chemotherapy (NAC) remains limited. Moreover, there are currently no biomarkers enabling an individual prediction of therapeutic efficacy. The aim of this study was the identification of serum biomarkers of early response to NAC. Methods: We conducted this prospective study in the MSCNRIO in Warsaw, Poland. A total of 71 patients and 15 healthy volunteers gave informed consent. Complete blood count, carcinoembryonic antigen (CEA), carcinoma antigen 125 (CA125), carcinoma antigen 19.9 (CA19.9), and fibrinogen (F) were measured at baseline and before every cycle. Circulating tumour cells (CTCs) and interleukin-1β (IL-1β), interleukin-6 (IL-6), interleukin-8 (IL-8), and interleukin-10 (IL-10) were measured in a pilot group of 40 patients at baseline and before cycle two (C2) and cycle three (C3). Results: Of all the measured parameters, only the IL-6 serum level was statistically significant. The IL-6 level before C2 of chemotherapy was significantly decreased in the complete pathological response (pCR) vs. the non-pCR group (3.71 pg/mL vs. 7.63 pg/mL, p = 0.004). In all patients with an IL-6 level below 5.0 pg/mL in C2, tumour regression TRG1a/1b according to the Becker classification and ypN0 were detected in postoperative histopathological specimens. The IL-6 level before C1 of chemotherapy was significantly elevated in ypN+ vs. ypN0 (7.69 pg/mL vs. 2.89 pg/mL, p = 0.022). Conclusions: The trial showed that an elevated level of IL-6 prior to treatment and C2 might be a predictor of pathological response to NAC.
Endoscopic Ultrasound-Guided Fine-Needle Biopsy Versus Aspiration for Tissue Sampling Adequacy for Molecular Testing in Pancreatic Ductal Adenocarcinoma
Authors: Mohamed Wael T. , Jahagirdar Vinay , Jaber Fouad , Ahmed Mohamed K. , Fatima Ifrah , Bierman Thomas , Fu Zhuxuan , Jones Philip G. , Hassan Amira F. , Faber Erin , Clarkston Wendell K. , Ghoz Hassan , Tawfik Ossama W. , Jonnalagadda Sreeni
Published: February 2024

Background and Aims: There is limited literature on sample adequacy for molecular testing in pancreatic ductal adenocarcinoma obtained via endoscopic ultrasound (EUS) fine-needle aspiration (FNA) versus EUS fine-needle biopsy (FNB). We aimed to compare these two modalities regarding sample adequacy for molecular and genomic sequencing. Methods: We reviewed all patients with pancreatic ductal adenocarcinoma who underwent EUS at Saint Luke’s Hospital from 2018 to 2021. The patients were categorized based on the method of EUS tissue acquisition, specifically FNA or FNB. A comprehensive evaluation was conducted for all cases by cytotechnologists. Results: Out of 132 patients who underwent EUS-guided biopsies, 76 opted for FNA, 48 opted for FNB, and 8 opted for a combination of both. The average number of passes required for FNB and FNA was 2.58 ± 1.06 and 2.49 ± 1.07, respectively (p = 0.704), indicating no significant difference. Interestingly, 71.4% (35) of FNB-obtained samples were deemed adequate for molecular testing, surpassing the 32.1% (26) adequacy observed with FNA (p < 0.001). Additionally, 46.4% (26) of FNB-obtained samples were considered adequate for genomic testing, a notable improvement over the 23.8% (20) adequacy observed with FNA (p = 0.005). Conclusion: Although the number of passes required for cytologic diagnosis did not differ significantly between EUS-FNB and EUS-FNA, the former demonstrated superiority in obtaining samples adequate for molecular testing. Tumor surface area and cellularity were crucial parameters in determining sample adequacy for molecular testing, irrespective of the chosen tissue acquisition modality.
Regulating the Hydrodeoxygenation Activity of Molybdenum Carbide with Different Diamines as Carbon Sources
Authors: Zhou Linyuan , Yang Huiru , Du Xiangze , Hu Changwei
Published: February 2024

The hydrodeoxygenation (HDO) of renewable fats or fatty acids into alkanes is a powerful measure to address energy and environmental crises. Molybdenum carbide-based catalysts are promising due to their platinum-like noble metal electronic properties. In this paper, Mo2C catalysts were prepared by one-step carbonization of amine molybdenum oxide (AMO) precursors using diamines with different carbon chain lengths as ligands. The physical and chemical properties and the HDO catalytic activity of the catalysts were investigated. The results indicate that as the carbon chain of diamines in the precursor increases, the carbon content of the catalysts in the surface and bulk phase increases. The Mo2C-12 catalyst exhibited excellent catalytic performance, with a palmitic acid conversion rate of 100% and an alkane selectivity of 96.6%, which are attributed to the smallest particle size, largest pore size, and synergistic effect of carbon. This work provides a simple and safe method for regulating the surface properties of Mo2C catalysts.
Influence of Impurities in the Chemical Processing Chain of Biomass on the Catalytic Valorisation of Cellulose towards γ-Valerolactone
Authors: Kashyap Preeti , Brzezińska Magdalena , Keller Nicolas , Ruppert Agnieszka
Published: February 2024

The conversion of lignocellulosic biomass to valuable chemicals such as levulinic acid and γ-valerolactone is a promising approach for achieving a sustainable circular economy. However, the presence of impurities during the stepwise chemical processing chain of the biomass feedstock can significantly impact both the hydrolysis and hydrogenation steps implemented to convert the cellulosic feedstock to levulinic acid and further to γ-valerolactone, respectively. This review article explores the effects of those impurities by classifying them into two groups, namely endogenous and exogenous types, based on whether they originate directly from the raw lignocellulosic biomass or arise during its multi-step chemical processing. Endogenous impurities include heavy metals, alkali metals, alkaline earth metals, proteins, and side products from the downstream treatment of cellulose, while exogenous impurities are introduced during physical pre-treatments such as ball milling or during the hydrolysis step, or they might originate from the reactor setup. The specific catalyst deactivation by carbonaceous species such as humins and coke is considered. The mechanisms of impurity-induced catalyst deactivation and by-product formation are thoroughly discussed. Additionally, strategies for minimizing the detrimental effects of impurities on biomass conversion and enhancing catalytic efficiency and stability are also proposed.
Fourier Ptychographic Microscopy 10 Years on: A Review
Authors: Xu Fannuo , Wu Zipei , Tan Chao , Liao Yizheng , Wang Zhiping , Chen Keru , Pan An
Published: February 2024

Fourier ptychographic microscopy (FPM) emerged as a prominent imaging technique in 2013, attracting significant interest due to its remarkable features such as precise phase retrieval, expansive field of view (FOV), and superior resolution. Over the past decade, FPM has become an essential tool in microscopy, with applications in metrology, scientific research, biomedicine, and inspection. This achievement arises from its ability to effectively address the persistent challenge of achieving a trade-off between FOV and resolution in imaging systems. It has a wide range of applications, including label-free imaging, drug screening, and digital pathology. In this comprehensive review, we present a concise overview of the fundamental principles of FPM and compare it with similar imaging techniques. In addition, we present a study on achieving colorization of restored photographs and enhancing the speed of FPM. Subsequently, we showcase several FPM applications utilizing the previously described technologies, with a specific focus on digital pathology, drug screening, and three-dimensional imaging. We thoroughly examine the benefits and challenges associated with integrating deep learning and FPM. To summarize, we express our own viewpoints on the technological progress of FPM and explore prospective avenues for its future developments.
Defining Transcriptomic Heterogeneity between Left and Right Ventricle-Derived Cardiac Fibroblasts
Authors: Dewar Michael Bradley , Ehsan Fahad , Izumi Aliya , Zhang Hangjun , Zhou Yu-Qing , Shah Haisam , Langburt Dylan , Suresh Hamsini , Wang Tao , Hacker Alison , Hinz Boris , Gillis Jesse , Husain Mansoor , Heximer Scott Patrick
Published: February 2024

Cardiac fibrosis is a key aspect of heart failure, leading to reduced ventricular compliance and impaired electrical conduction in the myocardium. Various pathophysiologic conditions can lead to fibrosis in the left ventricle (LV) and/or right ventricle (RV). Despite growing evidence to support the transcriptomic heterogeneity of cardiac fibroblasts (CFs) in healthy and diseased states, there have been no direct comparisons of CFs in the LV and RV. Given the distinct natures of the ventricles, we hypothesized that LV- and RV-derived CFs would display baseline transcriptomic differences that influence their proliferation and differentiation following injury. Bulk RNA sequencing of CFs isolated from healthy murine left and right ventricles indicated that LV-derived CFs may be further along the myofibroblast transdifferentiation trajectory than cells isolated from the RV. Single-cell RNA-sequencing analysis of the two populations confirmed that Postn+ CFs were more enriched in the LV, whereas Igfbp3+ CFs were enriched in the RV at baseline. Notably, following pressure overload injury, the LV developed a larger subpopulation of pro-fibrotic Thbs4+/Cthrc1+ injury-induced CFs, while the RV showed a unique expansion of two less-well-characterized CF subpopulations (Igfbp3+ and Inmt+). These findings demonstrate that LV- and RV-derived CFs display baseline subpopulation differences that may dictate their diverging responses to pressure overload injury. Further study of these subpopulations will elucidate their role in the development of fibrosis and inform on whether LV and RV fibrosis require distinct treatments.
Structure and Function of Dynein’s Non-Catalytic Subunits
Authors: Rao Lu , Gennerich Arne
Published: February 2024

Dynein, an ancient microtubule-based motor protein, performs diverse cellular functions in nearly all eukaryotic cells, with the exception of land plants. It has evolved into three subfamilies—cytoplasmic dynein-1, cytoplasmic dynein-2, and axonemal dyneins—each differentiated by their cellular functions. These megadalton complexes consist of multiple subunits, with the heavy chain being the largest subunit that generates motion and force along microtubules by converting the chemical energy of ATP hydrolysis into mechanical work. Beyond this catalytic core, the functionality of dynein is significantly enhanced by numerous non-catalytic subunits. These subunits are integral to the complex, contributing to its stability, regulating its enzymatic activities, targeting it to specific cellular locations, and mediating its interactions with other cofactors. The diversity of non-catalytic subunits expands dynein’s cellular roles, enabling it to perform critical tasks despite the conservation of its heavy chains. In this review, we discuss recent findings and insights regarding these non-catalytic subunits.
In Vivo Cellular Phosphatidylcholine Kinetics of CD15+ Leucocytes and CD3+ T-Lymphocytes in Adults with Acute Respiratory Distress Syndrome
Authors: Dushianthan Ahilanandan , Cusack Rebecca , Goss Victoria , Koster Grielof , Grocott Michael P. W. , Postle Anthony D.
Published: February 2024

Mammalian cell membranes composed of a mixture of glycerophospholipids, the relative composition of individual phospholipids and the dynamic flux vary between cells. In addition to their structural role, membrane phospholipids are involved in cellular signalling and immunomodulatory functions. In this study, we investigate the molecular membrane composition and dynamic flux of phosphatidylcholines in CD15+ leucocytes and CD3+ lymphocytes extracted from patients with acute respiratory distress syndrome (ARDS). We identified compositional variations between these cell types, where CD15+ cells had relatively higher quantities of alkyl-acyl PC species and CD3+ cells contained more arachidonoyl-PC species. There was a significant loss of arachidonoyl-PC in CD3+ cells in ARDS patients. Moreover, there were significant changes in PC composition and the methyl-D9 enrichment of individual molecular species in CD15+ cells from ARDS patients. This is the first study to perform an in vivo assessment of membrane composition and dynamic changes in immunological cells from ARDS patients.
Fall and Rise: Disentangling Cycle Life Trends in Atmospheric Plasma-Synthesized FeOOH/PANI Composite for Conversion Anodes in Lithium-Ion Batteries
Authors: Beletskii Evgenii V. , Volkov Alexey I. , Kharisova Ksenia A. , Glumov Oleg V. , Kamarou Maksim A. , Lukyanov Daniil A. , Levin Oleg V.
Published: February 2024

Various iron oxides have been proven to be promising anode materials for metal-ion batteries due to their natural abundance, high theoretical capacity, ease of preparation, and environmental friendliness. However, the synthesis of iron oxide-based composites requires complex approaches, especially when it comes to composites with intrinsically conductive polymers. In this work, we propose a one-step microplasma synthesis of polyaniline-coated urchin-like FeOOH nanoparticles (FeOOH/PANI) for applications as anodes in lithium-ion batteries. The material shows excellent electrochemical properties, providing an initial capacity of ca. 1600 mA∙h∙g−1 at 0.05 A∙g−1 and 900 mA∙g−1 at 1.2 A∙g−1. Further cycling led to a capacity decrease to 150 mA∙h∙g−1 by the 60th cycle, followed by a recovery that maintained the capacity at 767 mA∙h∙g−1 after 2000 cycles at 1.2 A∙g−1 and restored the full initial capacity of 1600 mA∙h∙g−1 at a low current density of 0.05 A∙g−1. Electrochemical milling—the phenomenon we confirmed via a combination of physico-chemical and electrochemical techniques—caused the material to exhibit interesting behavior. The anodes also exhibited high performance in a full cell with NMC532, which provided an energy density of 224 Wh∙kg−1, comparable to the reference cell with a graphite anode (264 Wh∙kg−1).
The Effect of Oral Iron Supplementation/Fortification on the Gut Microbiota in Infancy: A Systematic Review and Meta-Analysis
Authors: Karamantziani Theoni , Pouliakis Abraham , Xanthos Theodoros , Ekmektzoglou Konstantinos , Paliatsiou Styliani , Sokou Rozeta , Iacovidou Nicoletta
Published: February 2024

(1) Background: Iron is an essential metal for the proper growth and neurodevelopment of infants. To prevent and treat iron deficiency, iron supplementation or fortification is often required. It has been shown, though, that it affects the synthesis of gut microbiota. (2) Methods: This paper is a systematic review and meta-analysis of the effect of oral iron supplementation/fortification on the gut microbiota in infancy. Studies in healthy neonates and infants who received per os iron with existing data on gut microbiota were included. Three databases were searched: PUBMED, Scopus, and Google Scholar. Randomized controlled trials (RCTs) were included. Quality appraisal was assessed using the ROB2Tool. (3) Results: A total of six RCTs met inclusion criteria for a systematic review, and four of them were included in the meta-analysis using both the fixed and random effects methods. Our results showed that there is very good heterogeneity in the iron group (I2 = 62%), and excellent heterogeneity in the non-iron group (I2 = 98%). According to the meta-analysis outcomes, there is a 10.3% (95% CI: −15.0–−5.55%) reduction in the bifidobacteria population in the iron group and a −2.96% reduction for the non-iron group. There is a confirmed difference (p = 0.02) in the aggregated outcomes between iron and non-iron supplement, indicative that the bifidobacteria population is reduced when iron supplementation is given (total reduction 6.37%, 95%CI: 10.16–25.8%). (4) Conclusions: The abundance of bifidobacteria decreases when iron supplementation or fortification is given to infants.
Annual Solar Geoengineering: Mitigating Yearly Global Warming Increases
Authors: Feinberg Alec
Published: February 2024

Solar geoengineering (SG) solutions have many advantages compared to the difficulty of carbon dioxide removal (CDR): SG produces fast results, is shown here to have much higher efficiency than CDR, is not related to fossil fuel legislation, reduces the GHG effect including water vapor, and is something we all can participate in by brightening the Earth with cool roofs and roads. SG requirements detailed previously to mitigate global warming (GW) have been concerning primarily because of overwhelming goals and climate circulation issues. In this paper, annual solar geoengineering (ASG) equations and estimated requirements for yearly solar radiation modification (SRM) of areas are provided along with the advantages of annual solar geoengineering (ASG) to mitigate yearly global warming temperature increases. The ASG albedo area modification requirements found here are generally 50 to potentially more than 150 times less compared to the challenge of full SG GW albedo mitigation, reducing circulation concerns and increasing feasibility. These reductions are applied to L1 space sunshading, Earth brightening, and stratosphere aerosol injection (SAI) SRM annual area requirements. However, SAI coverage compared to other methods will have higher yearly increasing maintenance costs in the annual approach. Results also show that because ASG Earth albedo brightening area requirements are much smaller than those needed for full mitigation, there are concerns that worldwide negative SG would interfere with making positive advances for several reasons. That is, negative SG currently dominates yearly practices with the application of dark asphalt roads, roofs, and building sides. This issue is discussed.
The Impact of Education Level on Individual Lifestyle Behaviors among Dietetics Students and Professionals
Authors: Popiolek-Kalisz Joanna , Cakici Cansu , Szczygiel Karolina , Przytula Agata
Published: February 2024

Lifestyle and habits are acquired in the family environment and then shaped by the potential influence of the environment and received education. In recent years, there has been growing interest in understanding the relationship between sleep and dietary behaviors in various health professionals, including medical and dietetics professionals and students, as well as their self-perceived knowledge and attitudes. Despite the importance of this topic, there is a lack of research on the assessment of individual behaviors in dietetics students and professionals. The aim of this study was to assess the impact of education level on individual behaviors regarding nutrition, sleep, and physical activity in dietetics students and professionals. 71 dietetics students and professionals were enrolled in this study. Their overall knowledge, sleep, and nutritional behavior were assessed with a validated Questionnaire of Eating Behaviors at the beginning of their dietetics university education and then prospectively after a year. It was also compared to dieticians who already graduated. The analysis showed that the educational level did not correlate with sleep length or the physical activity level. However, the educational level was correlated with dietary knowledge and properly self-assessed by the participants. Significant differences were observed in both the prospective and comparative analyses. The educational level and knowledge were not correlated with eating behaviors. The self-assessment of nutritional behaviors also did not correlate with the objective assessment. Sleep length did not correlate with BMI, but it was inversely correlated with overall and healthy diet scores and knowledge levels. On the other hand, physical activity levels were positively correlated with healthy diet scores. Dietary education results in better nutritional knowledge; however, it does not significantly impact individual nutritional behaviors among dietetics students and professionals. Moreover, the inverse relationship between sleep length and nutritional knowledge and behaviors, as well as the positive relationship between physical activity level and dietary behaviors, shows that nutritional aspects of lifestyle are probably prioritized among dietetic students and professionals, with an acknowledgment of the role of physical activity and a neglect of sleep hygiene importance. Dietetics students should be advised to use their theoretical knowledge not only to guide their patients but also to implement it in their own lives.
Albumin–Rutin Nanoparticles: Design, Characterization, and Biophysical Evaluation
Authors: Chilom Claudia G. , Balan Adriana Elena , Enache Teodor Adrian , Oprea Daniela , Enculescu Monica , Florescu Monica , David Melinda
Published: February 2024

The bioavailability of the administered drugs that reach the systemic circulation is the first point in resolving the pathology of patients. Albumin-based nanoparticles represent an increasingly used strategy to deliver cancer drugs into cells that otherwise cannot overcome biological barriers. In this work, rutin (Ru), a flavonoid with anticancer and antioxidant potential, was incorporated into bovine serum albumin nanoparticles (BSA-Ru NPs), developed using the desolvation method, and the entire system was characterized and evaluated by scanning electron microscopy (SEM), atomic force microscopy (AFM), and UV–Vis absorption spectroscopy. The results showed that BSA and BSA–Ru NPs are uniformly distributed, have relatively large sizes, and have a time stability of more than 60%. Furthermore, the effect of these nanohybrids on the thermal stability of liposomal membranes was evaluated by surface plasmon resonance (SPR), cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS). The viability evaluation was assessed by the tetrazolium salt (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) (MTS) protocol in the fibroblast L929 line and a high level of biocompatibility, confirmed by SEM results, was found.
Microstructure and Dry-Sliding Wear Resistance of CoCrFeNiMoTix High Entropy Alloy Coatings Produced by Laser Cladding
Authors: Liang Hui , Hou Jinxin , Jiang Li , Cao Zhiqiang
Published: February 2024

The new-type CoCrFeNiMoTix high-entropy alloy coatings were successfully devised and prepared on Q235 steel using laser cladding. Influence of Ti content on their microstructure and wear-resistance was studied systematically; the relevant mechanisms were deeply revealed. The CoCrFeNiMoTix coatings consisted of NiTi, FCC, and BCC phases, and with the increasing of Ti content, contents of BCC phase and FCC phase gradually increased and decreased, respectively. The CoCrFeNiMoTi0.75 coating had the highest hardness (950 HV), which was about 6.5 times higher than the substrate (Q235 steel, 150 HV). According to Archard law, metal materials’ wear resistance is generally proportional to hardness; thus, the CoCrFeNiMoTi0.75 high entropy alloy coating with the highest hardness showed the best wear resistance, exhibiting a wear mechanism of slight abrasive wear.
Dynamic Compression and Constitutive Model in Fe-27Mn-10Al-1C Duplex Lightweight Steel
Authors: Cao Pengfei , Li Dazhao , Bai Shaobin , Chen Yongan , Lu Haitao
Published: February 2024

Fe-Mn-Al-C lightweight steels have been of significant interest due to their excellent mechanical properties and unique microstructures. However, there has been limited focus on the dynamic deformation. Here, we systematically investigate the mechanical responses over various strain rates and corresponding microstructure evolution in quasi-static and dynamic compression to reveal the transition of deformation mechanisms. The present lightweight steel exhibits a significant strain rate effect, with the yield strength increasing from 735.8 to 1149.5 MPa when the strain rate increases from 10−3 to 3144 s−1. The deformation in ferrite under high-strain-rate loading is dominated by wave slip, forming a cellular structure (cell block). Meanwhile, the deformation in austenite is dominated by planar slip, forming dislocation substructures such as high-density dislocation walls and microbands. In addition, the deformation twinning (including secondary twinning)- and microband-induced plasticity effects are responsible for the excellent dynamic compression properties. This alloy delays damage location while maintaining high strength, making it ideal for shock loading and high-strain-rate applications. The Johnson–Cook (J–C) constitutive model is used to predict the deformation behavior of lightweight steel under dynamic conditions, and the J–C model agrees well with the experimental results.
Insights into the Electronic, Optical, and Anti-Corrosion Properties of Two-Dimensional ZnO: First-Principles Study
Authors: Elwahab Fatma Abd , Teleb Nahed H. , Abdelsalam Hazem , Abd-Elkader Omar H. , Zhang Qinfang
Published: February 2024

The electronic, optical, and anticorrosion properties of planer ZnO crystal and quantum dots are explored using density functional theory calculations. The calculations for the finite ZnO quantum dots were performed in Gaussian 16 using the B3LYP/6-31g level of theory. The periodic calculations were carried out using VASP with the plane wave basis set and the PBE functional. The subsequent band structure calculations were performed using the hybrid B3LYP functional that shows accurate results and is also consistent with the finite calculations. The considered ZnO nanodots have planer hexagonal shapes with zigzag and armchair terminations. The binding energy calculations show that both structures are stable with negligible deformation at the edges. The ZnO nanodots are semiconductors with a moderate energy gap that decreases when increasing the size, making them potential materials for anticorrosion applications. The values of the electronic energy gaps of ZnO nanodots are confirmed by their UV-Vis spectra, with a wide optical energy gap for the small structures. Additionally, the calculated positive fraction of transferred electrons implies that electron transfer occurs from the inhibitor (ZnO) to the metal surface to passivate their vacant d-orbitals, and eventually prevent corrosion. The best anti-corrosion performance was observed in the periodic ZnO crystal with a suitable energy gap, electronegativity, and fraction of electron transfer. The effects of size and periodicity on the electronic and anticorrosion properties are also here investigated. The findings show that the anticorrosion properties were significantly enhanced by increasing the size of the quantum dot. Periodic ZnO crystals with an appropriate energy gap, electronegativity, and fraction of electron transfer exhibited the optimum anticorrosion performance. Thus, the preferable energy gap in addition to the most promising anticorrosion parameters imply that the monolayer ZnO is a potential candidate for coating and corrosion inhibitors.
A Special Issue on the Diversity, Ecology and Evolution of Dragonflies and Damselflies (Insecta: Odonata)
Authors: Lorenzo-Carballa M. Olalla , Koroiva Ricardo
Published: February 2024

The Odonata is an order of insects commonly known as dragonflies and damselflies, with a worldwide distribution except in Antarctica [...]
Draft Genome Sequencing of the Bacillus thuringiensis var. Thuringiensis Highly Insecticidal Strain 800/15
Authors: Shikov Anton E. , Savina Iuliia A. , Romanenko Maria N. , Nizhnikov Anton A. , Antonets Kirill S.
Published: February 2024

The Bacillus thuringiensis serovar thuringiensis strain 800/15 has been actively used as an agent in biopreparations with high insecticidal activity against the larvae of the Colorado potato beetle Leptinotarsa decemlineata and gypsy moth Lymantria dispar. In the current study, we present the first draft genome of the 800/15 strain coupled with a comparative genomic analysis of its closest reference strains. The raw sequence data were obtained by Illumina technology on the HiSeq X platform and de novo assembled with the SPAdes v3.15.4 software. The genome reached 6,524,663 bp. in size and carried 6771 coding sequences, 3 of which represented loci encoding insecticidal toxins, namely, Spp1Aa1, Cry1Ab9, and Cry1Ba8 active against the orders Lepidoptera, Blattodea, Hemiptera, Diptera, and Coleoptera. We also revealed the biosynthetic gene clusters responsible for the synthesis of secondary metabolites, including fengycin, bacillibactin, and petrobactin with predicted antibacterial, fungicidal, and growth-promoting properties. Further comparative genomics suggested the strain is not enriched with genes linked with biological activities implying that agriculturally important properties rely more on the composition of loci rather than their abundance. The obtained genomic sequence of the strain with the experimental metadata could facilitate the computational prediction of bacterial isolates’ potency from genomic data.
A Comparative Study of Transcranial Color-Coded Doppler (TCCD) and Transcranial Doppler (TCD) Ultrasonography Techniques in Assessing the Intracranial Cerebral Arteries Haemodynamics
Authors: Gunda Simon Takadiyi , Ng Tsam Kit Veronica , Liu Tsz-Ying , Chen Ziman , Han Xinyang , Chen Xiangyan , Pang Marco Yiu-Chung , Ying Michael Tin-Cheung
Published: February 2024

Cerebrovascular disease (CVD) poses a major public health and socio-economic burden worldwide due to its high morbidity and mortality rates. Accurate assessment of cerebral arteries’ haemodynamic plays a crucial role in the diagnosis and treatment management of CVD. The study compared a non-imaging transcranial Doppler ultrasound (TCD) and transcranial color-coded Doppler ultrasound (with (cTCCD) and without (ncTCCD)) angle correction in quantifying middle cerebral arteries (MCAs) haemodynamic parameters. A cross-sectional study involving 50 healthy adults aged ≥ 18 years was conducted. The bilateral MCAs were insonated via three trans-temporal windows (TTWs—anterior, middle, and posterior) using TCD, cTCCD, and ncTCCD techniques. The MCA peak systolic velocity (PSV) and mean flow velocity (MFV) were recorded at proximal and distal imaging depths that could be visualised on TCCD with a detectable spectral waveform. A total of 152 measurements were recorded in 41 (82%) subjects with at least one-sided open TTW across the three techniques. The mean PSVs measured using TCD, ncTCCD, and cTCCD were 83 ± 18 cm/s, 81 ± 19 cm/s, and 93 ± 21 cm/s, respectively. There was no significant difference in PSV between TCD and ncTCCD (bias = 2 cm/s, p = 1.000), whereas cTCCD yielded a significantly higher PSV than TCD and ncTCCD (bias = −10 cm/s, p < 0.001; bias = −12 cm/s, p ≤ 0.001, respectively). The bias in MFV between TCD and ncTCCD techniques was (bias = −0.5 cm/s; p = 1.000), whereas cTCCD demonstrated a higher MFV compared to TCD and ncTCCD (bias = −8 cm/s, p < 0.001; bias = −8 cm/s, p ≤ 0.001, respectively). TCCD is a practically applicable imaging technique in assessing MCA blood flow velocities. cTCCD is more accurate and tends to give higher MCA blood flow velocities than non-imaging TCD and ncTCCD techniques. ncTCCD is comparable to non-imaging TCD and should be considered in clinical cases where using both TCD and TCCD measurements is needed.
The Role of Ultrasonography in the Process of Weaning from Mechanical Ventilation in Critically Ill Patients
Authors: Al-Husinat Lou’i , Jouryyeh Basil , Rawashdeh Ahlam , Robba Chiara , Silva Pedro , Rocco Patricia , Battaglini Denise
Published: February 2024

Weaning patients from mechanical ventilation (MV) is a complex process that may result in either success or failure. The use of ultrasound at the bedside to assess organs may help to identify the underlying mechanisms that could lead to weaning failure and enable proactive measures to minimize extubation failure. Moreover, ultrasound could be used to accurately identify pulmonary diseases, which may be responsive to respiratory physiotherapy, as well as monitor the effectiveness of physiotherapists’ interventions. This article provides a comprehensive review of the role of ultrasonography during the weaning process in critically ill patients.
Understanding Loss to Follow-Up in AMD Patients Receiving VEGF Inhibitor Therapy: Associated Factors and Underlying Reasons
Authors: Kusenda Pavol , Caprnda Martin , Gabrielova Zuzana , Kukova Natalia , Pavlovic Samuel , Stefanickova Jana
Published: February 2024

Background: In patients with wet age-related macular degeneration (AMD), loss to follow-up (LTFU) leads to unplanned interruptions in therapy and the risk of visual loss. Methods: This retrospective and prospective case–control cohort study compared AMD patients with (LTFU YES) and without (LTFU NO) LTFU during anti-VEGF treatment over 12 years. LTFU was defined as missing any treatment or monitoring visits, or not scheduling follow-ups for six months. Results: Significant differences between LTFU NO (n = 298) and LTFU YES (n = 174) groups were age, treatment phase, baseline and final best-corrected visual acuity (BCVA), type of anti-VEGF drug, treatment switch, commuting distance, and escort during commuting. A multivariate logistic regression analysis identified the need for an escort during the commuting and treatment phase as the only significant difference. The four most common reasons for LTFU were general health worsening (21.8%), patient-missed appointments (16.7%), COVID-19-related issues (14.9%), and treatment dissatisfaction (8.6%). Conclusions: The factors associated with increased LTFU rates were older age, inactive treatment phase, lower baseline and final BCVA, bevacizumab treatment, monotherapy, longer travelling distance, and commuting with an escort. According to the multivariate logistic regression analysis, only the escort during the commuting and treatment phases was significant. These findings could direct research to explore social support in treatment adherence and highlight the importance of treatment phases in practice.
Ultrasound-Guided Interventions in the Biliary System
Authors: Müller Thomas , Braden Barbara
Published: February 2024

Ultrasound guidance in biliary interventions has become the standard tool to facilitate percutaneous biliary drainage as well as percutaneous gall bladder drainage. Monitoring of the needle tip whilst penetrating the tissue in real time using ultrasound allows precise manoeuvres and exact targeting without radiation exposure. Without the need for fluoroscopy, ultrasound-guided drainage procedures can be performed bedside as a sometimes life-saving procedure in patients with severe cholangitis/cholecystitis when they are critically ill in intensive care units and cannot be transported to a fluoroscopy suite. This article describes the current data background and guidelines and focuses on specific sonographic aspects of both the procedures of percutaneous biliary drainage and gallbladder drainage.
Path Planning for Fixed-Wing Unmanned Aerial Vehicles: An Integrated Approach with Theta* and Clothoids
Authors: Bassolillo Salvatore Rosario , Raspaolo Gennaro , Blasi Luciano , D’Amato Egidio , Notaro Immacolata
Published: February 2024

Unmanned Aerial Vehicles (UAVs) have emerged as a compelling alternative to manned operations, offering the capability to navigate hazardous environments without risks for human operators. Despite their potential, optimizing UAV missions in complex and unstructured environments remains a pivotal challenge. Path planning becomes a crucial aspect to increase mission efficiency, although it is inherently complex due to various factors such as obstacles, no-fly zones, non-cooperative aircraft, and flight mechanics limitations. This paper presents a path-planning technique for fixed-wing unmanned aerial vehicles (UAVs) based on the Theta* algorithm. The approach introduces innovative features, such as the use of Euler spiral, or clothoids, to serve as connection arcs between nodes, mitigating trajectory discontinuities. The design of clothoids can be linked to the aircraft performance model, establishing a connection between curvature constraints and the specific characteristics of the vehicle. Furthermore, to lower the computational burden, the implementation of an adaptive exploration distance and a vision cone was considered, reducing the number of explored solutions. This methodology ensures a seamless and optimized flight path for fixed-wing UAVs operating in static environments, showcasing a noteworthy improvement in trajectory smoothness. The proposed methodology has been numerically evaluated in several complex test cases as well as in a real urban scenario to prove its effectiveness.
A Hybrid Cryptosystem Incorporating a New Algorithm for Improved Entropy
Authors: Silva-García Víctor Manuel , Flores-Carapia Rolando , Cardona-López Manuel Alejandro
Published: February 2024

Today, safeguarding sensitive content through encryption is crucial. This work presents a hybrid cryptosystem for images that employs both asymmetric and symmetric encryption. The asymmetric component involves applying the Diffie–Hellman protocol and the ElGamal cryptosystem to securely transmit two constants. These constants are necessary for the symmetrical aspect to generate dynamic permutations, substitution boxes, and round keys. Following an encryption process with fourteen rounds, the encrypted images are processed by an algorithm proposed to enhance entropy, a critical metric for assessing encryption quality. It increases the frequencies of the basic colors to achieve a histogram closely resembling a uniform distribution, but it increases the image size by approximately 8%. This improves the entropy values achieved by the hybrid cryptosystem, bringing them remarkably close to the ideal value of 8.0. In specific instances, the entropy values were elevated from 7.99926 to 8.0. The proposed method exhibits resilience against various attacks, including differential, linear, brute force, and algebraic attacks, as evaluated through the entropy, correlation, goodness of fit, Discrete Fourier Transform (DFT), Number of Pixels Change Rate (NPCR), Unified Average Changing Intensity (UACI), Avalanche Criteria (AC), contrast, energy, and homogeneity. Further, encrypted images are subjected to noise attacks ranging from 20% to 50% noise, including additive, multiplicative, occlusion noise, as well as the newly introduced χ2 noise. The noise damage is quantified using the proposed Similarity Parameter (SP), and a 3 × 3 median filter is employed to enhance the visual quality.
Entropy-Based Node Importance Identification Method for Public Transportation Infrastructure Coupled Networks: A Case Study of Chengdu
Authors: Zeng Ziqiang , Sun Yupeng , Zhang Xinru
Published: February 2024

Public transportation infrastructure is a typical, complex, coupled network that is usually composed of connected bus lines and subway networks. This study proposes an entropy-based node importance identification method for this type of coupled network that is helpful for the integrated planning of urban public transport and traffic flows, as well as enhancing network information dissemination and maintaining network resilience. The proposed method develops a systematic entropy-based metric based on five centrality metrics, namely the degree centrality (DC), betweenness centrality (BC), closeness centrality (CC), eigenvector centrality (EC), and clustering coefficient (CCO). It then identifies the most important nodes in the coupled networks by considering the information entropy of the nodes and their neighboring ones. To evaluate the performance of the proposed method, a bus–subway coupled network in Chengdu, containing 10,652 nodes and 15,476 edges, is employed as a case study. Four network resilience assessment metrics, namely the maximum connectivity coefficient (MCC), network efficiency (NE), susceptibility (S), and natural connectivity (NC), were used to conduct group experiments. The experimental results demonstrate the following: (1) the multi-functional fitting analysis improves the analytical accuracy by 30% as compared to fitting with power law functions only; (2) for both CC and CCO, the improved metric’s performance in important node identification is greatly improved, and it demonstrates good network resilience.
The Effects of Teacher-Centered and Student-Centered Approaches in TOEIC Reading Instruction
Authors: Busa Ji , Chung Sun-Joo
Published: February 2024

Teacher-centered instruction is prevalent in preparation courses for large-scale standardized language tests. Student-centered instruction, while known to be effective in enhancing communicative competence, has been considered ineffective and time-consuming to implement in preparation courses for large-scale standardized language tests. To investigate this assumption, a quasi-experimental study was conducted to investigate the effectiveness of two teaching approaches— teacher-centered and Jigsaw—on students’ Test of English for International Communication (TOEIC) Reading Comprehension Part Seven scores. The results showed that both groups experienced significant improvements from the pre-test to the post-test in their overall performance; however, there was no statistically significant difference between them. Furthermore, students in the Jigsaw group showed statistically significant improvements in all three passage types (single, double, and triple), while students in the teacher-centered group showed insignificant improvement in one of the three passage types (single passages). These findings suggest that both teaching approaches can effectively improve students’ TOEIC Reading Comprehension scores. The study adds to the body of research on teaching approaches in EFL TOEIC Reading Comprehension university classrooms. The findings suggest that teacher-centered and student-centered approaches can effectively improve students’ TOEIC Reading Comprehension scores.