bluuebunny/crossref_metadata_2025 huggingface dataset:
New Insights on Unique Features and Role of Nanostructured Materials in Cosmetics
Authors: Bilal Muhammad , Iqbal Hafiz M. N.
Published: April 2020

The cosmetics industry has boomed in recent years as one of the markets that holds enormous growth potential. Among several industrial sectors, the cosmetics industry has considered nanotechnology-based principles and implemented in product management practices. Out of 1000 registered products available on the global market, up to 13% were referred to as products for cosmetic use. A large number of nanoscale materials with unique physicochemical properties are currently being used in the cosmetics formulations or recommended for future use as nano-systems or novel nanocarriers to encapsulate active ingredients for their efficient delivery through the skin barriers. These nano-systems have demonstrated potential in targeted-oriented drug delivery and offered remarkable features such as better stability, site-specificity, excellent encapsulation efficiency, prolonged action, enhanced skin penetration, and high drug-loading capability. Nevertheless, nanotoxicology research has raised concerns over the excessive use of nanomaterials/nanoparticles in cosmetics, as nanoparticles might enter the skin resulting in health problems. This review provides insights on the characteristic physicochemical features and the potential use of various nanostructured materials, including liposomes, noisome, nanoemulsions, nanoparticles, carbon nanomaterials (graphene, fullerenes), carbon nanotubes, dendrimers, and nanospheres in cosmeceuticals. Moreover, the regulatory aspects of nanomaterials in cosmetics, along with concluding remarks and outlook in this field, were also vetted.
Functional Divergence Drives Invasibility of Plant Communities at the Edges of a Resource Availability Gradient
Authors: Tordoni Enrico , Petruzzellis Francesco , Nardini Andrea , Bacaro Giovanni
Published: April 2020

Invasive Alien Species (IAS) are a serious threat to biodiversity, severely affecting natural habitats and species assemblages. However, no consistent empirical evidence emerged on which functional traits or trait combination may foster community invasibility. Novel insights on the functional features promoting community invasibility may arise from the use of mechanistic traits, like those associated with drought resistance, which have been seldom included in trait-based studies. Here, we tested for the functional strategies of native and invasive assemblage (i.e., environmental filtering hypothesis vs. niche divergence), and we assessed how the functional space determined by native species could influence community invasibility at the edges of a resource availability gradient. Our results showed that invasive species pools need to have a certain degree of differentiation in order to persist in highly invaded communities, suggesting that functional niche divergence may foster community invasibility. In addition, resident native communities more susceptible to invasion are those which, on average, have higher resource acquisition capacity, and lower drought resistance coupled with an apparently reduced water-use efficiency. We advocate the use of a mechanistic perspective in future research to comprehensively understand invasion dynamics, providing also new insights on the factors underlying community invasibility in different ecosystems.
Public Key Encryption with Keyword Search in Cloud: A Survey
Authors: Zhou Yunhong , Li Na , Tian Yanmei , An Dezhi , Wang Licheng
Published: April 2020

With the popularization of cloud computing, many business and individuals prefer to outsource their data to cloud in encrypted form to protect data confidentiality. However, how to search over encrypted data becomes a concern for users. To address this issue, searchable encryption is a novel cryptographic primitive that enables user to search queries over encrypted data stored on an untrusted server while guaranteeing the privacy of the data. Public key encryption with keyword search (PEKS) has received a lot of attention as an important branch. In this paper, we focus on the development of PEKS in cloud by providing a comprehensive research survey. From a technological viewpoint, the existing PEKS schemes can be classified into several variants: PEKS based on public key infrastructure, PEKS based on identity-based encryption, PEKS based on attribute-based encryption, PEKS based on predicate encryption, PEKS based on certificateless encryption, and PEKS supporting proxy re-encryption. Moreover, we propose some potential applications and valuable future research directions in PEKS.
Genetic Analysis of Geothermal Resources and Geothermal Geological Characteristics in Datong Basin, Northern China
Authors: Zhou Wenlong , Hu Xiangyun , Yan Shilong , Guo Hongdang , Chen Wei , Liu Sijing , Miao Chunyan
Published: April 2020

Datong Basin is a Cenozoic fault basin located in the central part of the North China Block with strong tectonic activity. The unique geological environment of Datong Basin is believed to have good conditions for the formation of geothermal resources. Based on the research of the classification, genesis and geothermal geological characteristics of geothermal resources, the geological conditions, seismic activity, volcanic activity, geophysical exploration results, terrestrial heat flow and hot springs in Datong Basin are analyzed. The possibility of the occurrence of geothermal resources in Datong Basin is determined, and the genesis and occurrence mechanisms of geothermal resources in Datong Basin are judged. The results show that Datong Basin satisfies the geological geothermal conditions of the formation of geothermal resources and is of great research value. The formation of geothermal resources in the Datong Basin is affected by the uplift of the Qinghai–Tibet Plateau and the destruction of the North China Craton. The geothermal resources in Datong Basin are formed by the combination of modern volcanic activity and strong inner-plate tectonic activities. The geothermal system is a combination of convective hydrothermal systems and partial melt systems. At the same time, it is concluded that the key research areas for the occurrence of geothermal resources are mainly in the northeastern part of the basin. It is recommended to carry out detailed and comprehensive exploration of the northeastern part of Datong Basin.
Editorial on Special Issue “Wind Turbine Power Optimization Technology”
Authors: Castellani Francesco , Astolfi Davide
Published: April 2020

This Special Issue collects innovative contributions in the field of wind turbine optimization technology. The general motivation of the present Special Issue is given by the fact that there has recently been a considerable boost of the quest for wind turbine efficiency optimization in the academia and in the wind energy practitioners communities. The optimization can be focused on technology and operation of single turbine or a group of machines within a wind farm. This perspective is evidently multi-faced and the seven papers composing this Special Issue provide a representative picture of the most ground-breaking state of the art about the subject. Wind turbine power optimization means scientific research about the design of innovative aerodynamic solutions for wind turbine blades and of wind turbine single or collective control, especially for increasing rotor size and exploitation in offshore environment. It should be noticed that some recently developed aerodynamic and control solutions have become available in the industry practice and therefore an interesting line of development is the assessment of the actual impact of optimization technology for wind turbines operating in field: this calls for non-trivial data analysis and statistical methods. The optimization approach must be 360 degrees; for this reason also offshore resource should be addressed with the most up to date technologies such as floating wind turbines, in particular as regards support structures and platforms to be employed in ocean environment. Finally, wind turbine power optimization means as well improving wind farm efficiency through innovative uses of pre-existent control techniques: this is employed, for example, for active control of wake interactions in order to maximize the energy yield and minimize the fatigue loads.
Parametric Investigation of a Trigeneration System with an Organic Rankine Cycle and Absorption Heat Pump Driven by Parabolic Trough Collectors for the Building Sector
Authors: Bellos Evangelos , Tzivanidis Christos
Published: April 2020

This article presents a simulation study which focuses on the thermodynamic analysis of a solar-driven trigeneration system for heating, cooling, and electricity production. The system uses parabolic trough collectors operating with Therminol VP-1 for feeding an organic Rankine cycle operating with toluene and an absorption heat pump operating with a LiBr–H2O working pair. The collecting area is selected at 100 m2 and the storage tank at 4 m3. The system is studied parametrically in order to examine the impact of various parameters on the system energy efficiency, system exergy efficiency, electricity production, heating production, and cooling production in the simple payback period of the investment. The examined parameters are the following: solar beam irradiation level, solar beam irradiation angle, superheating degree in the turbine inlet, pressure level in the turbine inlet, heat source temperature level, generator temperature level, and the heat input in the generator. For the nominal case of a 15 kW generator input, the electricity production is 6.3 kW, the heating production 11.5 kW, and the cooling production 10.7 kW. The system energy efficiency is 40.7%, while the system exergy efficiency is 12.7%. The financial investigation of the investment proved that it is viable with the simple payback period to be 8.1 years in the nominal case and it can be reduced to 7.8 years with an optimization procedure. Lastly, it has to be said that the examined system is found to be a viable configuration which is an ideal choice for application in the building sector. The analysis was conducted under steady-state conditions with a model developed using Engineering Equation Solver (EES).
Structural Changes to Nuclear Energy Industries and the Economic Effects Resulting from Energy Transition Policies in South Korea
Authors: Kim Hana , Jeon Eui-Chan
Published: April 2020

The world’s aging fleet of nuclear power reactors faces decommissioning. Because decommissioning is implemented through a series of procedures, and this process changes the links between some industries, its economic impact needs to be investigated. This study compared the economic impacts of three nuclear industry sectors—nuclear generation, nuclear power construction, and nuclear decommissioning—using input-output analysis in South Korea, the first Asian country that has declared a nuclear phaseout. The study also traced changes in the structure of the nuclear industry during the Korean nuclear phaseout and the consequent economic impacts. The study found that the implementation of the nuclear phaseout will lead to a decrease in the induced outputs and the value added of nuclear industries in South Korea; however, this would be offset by deploying more renewable energy. In addition, the temporal impacts on individual sectors vary depending on changes in the number of reactors being decommissioned and the expenditure profile of the nuclear decommissioning sector. The findings call for policy measures that support a soft landing for nuclear industry sectors and related stakeholders, timely development of necessary technologies, and transparency in public communication. This study provides a methodological approach and input structure that can be used for studies in other countries.
Optimal Asset Planning for Prosumers Considering Energy Storage and Photovoltaic (PV) Units: A Stochastic Approach
Authors: Achiluzzi Eleonora , Kobikrishna Kirushaanth , Sivabalan Abenayan , Sabillon Carlos , Venkatesh Bala
Published: April 2020

In the distribution system, customers have increasingly use renewable energy sources and battery energy storage systems (BESS), transforming traditional loads into active prosumers. Therefore, methodologies are needed to provide prosumers with tools to optimize their investments and increase business opportunities. In this paper, a stochastic mixed integer linear programming (MILP) formulation is proposed to solve for optimal sizes of prosumer assets, considering the use of a BESS and photovoltaic (PV) units. The objective is to minimize the total cost of the system, which is defined as the combination of a solar PV system investment, BESS investment, maintenance costs of assets, and the cost of electricity supplied by the grid. The developed method defines the optimal size of PV units, the power/energy capacities of the BESS, and the optimal value for initial energy stored in the BESS. Both deterministic and stochastic approaches were explored. For each approach, the proposed model was tested for three cases, providing a varying combination of the use of grid power, PV units, and BESS. The optimal values from each case were compared, showing that there is potential to achieve more economic plans for prosumers when PV and BESS technologies are taken into account.
Effect of Bypass Control and Room Control Modes on Fan Energy Savings in a Heat Recovery Ventilation System
Authors: Cho Kyungjoo , Cho Dongwoo , Kim Taeyeon
Published: April 2020

This study makes a novel attempt to analyse the effect of the bypass control and room control modes on ventilation energy saving in an 84 m2 housing unit, which is the most frequently constructed unit-type among newly constructed apartment buildings in Korea. A heat recovery ventilation system was installed. The fan power consumption was measured via field experiments and analyses were made for potential energy savings. Experiments to confirm the power-saving effect owing to the application of the room control mode were performed under the heat recovery and bypass modes, using three air flow rates (0.5, 1.0 and 1.5 ACH). Additionally, the annual energy saving based on the application of the mixed mode (both bypass and room control modes) was calculated. The results obtained showed that when the mixed mode was employed, ventilation energy saving up to 10.76%–16.56%, which is greater than that obtained using only the heat recovery mode, was realized. Additionally, compared with all-room-ventilation, 26.69%–61.84% of ventilation energy could be saved if the mixed mode was applied only to the living room.
Analysis of Overvoltages Appearing in One-Sidedly Ungrounded MV Power Cable Screen
Authors: Schött-Szymczak Aleksandra , Walczak Krzysztof
Published: April 2020

Changing the connection type of a medium voltage (MV) cable screen is linked to a decrease in power loss, although it may also lead to the generation of overvoltage in a metallic cable screen, which is hazardous for proper cable line work. In this paper, results of simulation and field researches are presented, showing the range of voltages that occurs in cable screens during single-phase ground fault. There are considered to be three scenarios according to the applied type of cable screen connection. Conducted researches allow the comparison of chosen methods of simulation with an actual outcome. Results obtained during simulation and field studies lead to the conclusion that the range of overvoltage occurred in a cable screen could in fact be dangerous for the cable line’s sheath insulation without proper overvoltage protection.
Is It All about the Price? An Analysis of the Purchase Intention for Organic Food in a Discount Setting by Means of Structural Equation Modeling
Authors: Katt Felix , Meixner Oliver
Published: April 2020

In recent years, discount grocery retailers have expanded their global reach and added to their traditional no-frills offerings to also cater to hedonic consumer needs. In addition to a larger product assortment and a more pleasant shopping experience, they now sell organic food, which commands a price premium compared to non-organic alternatives. To understand organic food in a discount setting, this study sets out to examine the factors that influence discount grocery shoppers’ purchase intention for organic food. To study this relationship, this paper tests several factors in a structural equation model, finding a positive relationship between hedonic shopping values, environmental concern, as well as health consciousness and the purchase intention for organic food. In our model, based on a US consumer survey (n = 394), price consciousness exhibited a direct and negative relationship with the purchase intention for organic food. Furthermore, this study found that that the impact of environmental concern, health consciousness, and hedonic shopping value is greater on the purchase intention of organic food than that of price consciousness, even in this discount setting. This study concludes by discussing these results and attempting to outline potential areas for future research, as well as managerial implications.
Changes in Polyphenolics during Storage of Products Prepared with Freeze-Dried Wild Blueberry Powder
Authors: Lavefve Laura , Brownmiller Cindi , Howard Luke , Reeves Donovon , Adams Sean H. , Chen Jin-Ran , Diaz Eva C. , Mauromoustakos Andy
Published: April 2020

Wild blueberry (WBB) powder can be added to the formulation of foods to encourage consumption of health-promoting polyphenolics, but the stability of polyphenolics throughout storage is important. We determined the stability of polyphenolics in five products (ice pop, oatmeal bar, graham cracker cookie, juice, and gummy product) prepared with WBB powder. Samples stored at 21 °C, 4.4 °C, or −20 °C (ice pops only) were analyzed at 0, 2, 4, 6, and 8 weeks for polyphenolic content and percent polymeric color. Total anthocyanins decreased over storage and storage temperatures in all products. However, the ice pop and the refrigerated juice both retained over 90% of their initial total anthocyanin content. The refrigerated oatmeal bar also showed good retention of anthocyanins (86%), but the gummy product retained only 43% and 51% when stored at 4.4 °C or 21 °C, respectively. The lower amount of polyphenolic compounds recovered in the gummies stored at 4.4 °C compared to 21 °C may be attributed to reduced extraction efficiency as a result of gel hardening at refrigerated temperature. Chlorogenic acid and flavonols were generally more stable than anthocyanins throughout storage.
Integral Field Spectroscopy of Planetary Nebulae with MUSE
Authors: Walsh Jeremy R. , Monreal-Ibero Ana
Published: April 2020

The Multi-Unit Spectroscopic Explorer (MUSE) is a large integral field unit mounted on the ESO Very Large Telescope. Its spatial (60 arcsecond field) and wavelength (4800–9300Å) coverage is well suited to detailed imaging spectroscopy of extended planetary nebulae, such as in the Galaxy. An overview of the capabilities of MUSE applied to Planetary Nebulae (PNe) is provided together with the specific advantages and disadvantages. Some examples of archival MUSE observations of PNe are provided. MUSE datacubes for two targets (NGC 3132 and NGC 7009) are analyzed in detail, and they are used to show the advances achievable for planetary nebula studies. Prospects for further MUSE observations of PNe and a broader analysis of existing datasets are outlined.
Evaluation of Pediatric Surgical Site Infections Associated with Colorectal Surgeries at an Academic Children’s Hospital
Authors: Pough Kimberly , Bhakta Rima , Maples Holly , Honeycutt Michele , Vijayan Vini
Published: April 2020

Appropriate use of antibiotic prophylaxis (AP) is a key measure for the prevention of surgical site infections (SSI) in colorectal surgeries; however, despite the presence of national and international guidelines, compliance with AP recommendations remains low. The purpose of this study is to evaluate compliance with recommendations for the use of AP in children undergoing colorectal surgeries and to evaluate the effectiveness of antibiotics in the prevention of SSI. We collected demographic and clinical characteristics of patients who underwent colorectal surgeries, as well as microbiological and antimicrobial susceptibility data for patients who developed SSI. AP data were collected and compared with national guidelines. Antibiotic dosing and duration were most frequently in concordance with national guidelines, while antibiotic timing and selection had the lowest rates of compliance. Twelve of the 192 colorectal procedures evaluated resulted in SSI. Only 2 of the 12 children with SSI received appropriate AP for all four categories evaluated. Eight cases that resulted in SSI were due to organisms not covered by the recommended AP. We identified multiple areas for the improvement of AP in children undergoing colorectal surgery. A multidisciplinary approach to development of standardized protocols, educational interventions, and EHR-based algorithms may facilitate or improve appropriate AP use.
Finding Dr. Kim: Information Sources of Korean Immigrants’ Search for a Doctor in the U.S.
Authors: Jang Sou Hyun , Ko Linda K. , Meischke Hendrika
Published: April 2020

Korean immigrants in the United States (U.S.) are known for their preference for, and dependence on, co-ethnic doctors due to various barriers to the U.S. healthcare system. Recent immigrants tend to face more barriers than their non-recent counterparts. However, there is little information on how they find their doctors in the U.S. This study includes a self-administrated survey of Korean immigrants aged 18 and above who lived in the New York–New Jersey Metropolitan area in 2013–2014 (n = 440). Descriptive analysis was conducted to understand the most common information sources and the number of sources based on the duration of stay in the U.S. More recent Korean immigrants were female, had no family doctor, uninsured, younger, and more educated than their non-recent counterparts. Regardless of the duration of stay in the U.S., family members and friends were the most frequently sought-after sources for Korean immigrants in their search for doctors. In addition to family members and friends, non-recent Korean immigrants also used other methods (e.g., Korean business directories), whereas recent immigrants used both U.S. and Korean websites. More recent Korean immigrants used multiple sources compared to non-recent Korean immigrants, often combined with a Korean website. Our study suggests policy implications to improve recent immigrants’ accessibility to health information in a timely manner.
Electrophysiological Abnormalities in VLCAD Deficient hiPSC-Cardiomyocytes Can Be Improved by Lowering Accumulation of Fatty Acid Oxidation Intermediates
Authors: Knottnerus Suzan J. G. , Mengarelli Isabella , Wüst Rob C. I. , Baartscheer Antonius , Bleeker Jeannette C. , Coronel Ruben , Ferdinandusse Sacha , Guan Kaomei , IJlst Lodewijk , Li Wener , Luo Xiaojing , Portero Vincent M. , Ulbricht Ying , Visser Gepke , Wanders Ronald J. A. , Wijburg Frits A. , Verkerk Arie O. , Houtkooper Riekelt H. , Bezzina Connie R.
Published: April 2020

Patients with very long-chain acyl-CoA dehydrogenase deficiency (VLCADD) can present with life-threatening cardiac arrhythmias. The pathophysiological mechanism is unknown. We reprogrammed fibroblasts from one mildly and one severely affected VLCADD patient, into human induced pluripotent stem cells (hiPSCs) and differentiated these into cardiomyocytes (VLCADD-CMs). VLCADD-CMs displayed shorter action potentials (APs), more delayed afterdepolarizations (DADs) and higher systolic and diastolic intracellular Ca2+ concentration ([Ca2+]i) than control CMs. The mitochondrial booster resveratrol mitigated the biochemical, electrophysiological and [Ca2+]i changes in the mild but not in the severe VLCADD-CMs. Accumulation of potentially toxic intermediates of fatty acid oxidation was blocked by substrate reduction with etomoxir. Incubation with etomoxir led to marked prolongation of AP duration and reduced DADs and [Ca2+]i in both VLCADD-CMs. These results provide compelling evidence that reduced accumulation of fatty acid oxidation intermediates, either by enhanced fatty acid oxidation flux through increased mitochondria biogenesis (resveratrol) or by inhibition of fatty acid transport into the mitochondria (etomoxir), rescues pro-arrhythmia defects in VLCADD-CMs and open doors for new treatments.
Influence of Estrogens on Uterine Vascular Adaptation in Normal and Preeclamptic Pregnancies
Authors: Mandalà Maurizio
Published: April 2020

During pregnancy, the maternal cardiovascular system undergoes significant changes, including increased heart rate, cardiac output, plasma volume, and uteroplacental blood flow (UPBF) that are required for a successful pregnancy outcome. The increased UPBF is secondary to profound circumferential growth that extends from the downstream small spiral arteries to the upstream conduit main uterine artery. Although some of the mechanisms underlying uterine vascular remodeling are, in part, known, the factors that drive the remodeling are less clear. That higher circulating levels of estrogens are positively correlated with gestational uterine vascular remodeling suggests their involvement in this process. Estrogens binding to the estrogen receptors expressed in cytotrophoblast cells and in the uterine artery wall stimulate an outward hypertrophic remodeling of uterine vasculature. In preeclampsia, generally lower concentrations of estrogens limit the proper uterine remodeling, thereby reducing UPBF increases and restricting the growth of the fetus. This review aims to report estrogenic regulation of the maternal uterine circulatory adaptation in physiological and pathological pregnancy that favors vasodilation, and to consider the underlying molecular mechanisms by which estrogens regulate uteroplacental hemodynamics.
Keratin Dynamics and Spatial Distribution in Wild-Type and K14 R125P Mutant Cells—A Computational Model
Authors: Gouveia Marcos , Zemljič-Jokhadar Špela , Vidak Marko , Stojkovič Biljana , Derganc Jure , Travasso Rui , Liovic Mirjana
Published: April 2020

Keratins are one of the most abundant proteins in epithelial cells. They form a cytoskeletal filament network whose structural organization seriously conditions its function. Dynamic keratin particles and aggregates are often observed at the periphery of mutant keratinocytes related to the hereditary skin disorder epidermolysis bullosa simplex, which is due to mutations in keratins 5 and 14. To account for their emergence in mutant cells, we extended an existing mathematical model of keratin turnover in wild-type cells and developed a novel 2D phase-field model to predict the keratin distribution inside the cell. This model includes the turnover between soluble, particulate and filamentous keratin forms. We assumed that the mutation causes a slowdown in the assembly of an intermediate keratin phase into filaments, and demonstrated that this change is enough to account for the loss of keratin filaments in the cell’s interior and the emergence of keratin particles at its periphery. The developed mathematical model is also particularly tailored to model the spatial distribution of keratins as the cell changes its shape.
The Role of Erythroid Differentiation Regulator 1 (ERDR1) in the Control of Proliferation and Photodynamic Therapy (PDT) Response
Authors: Park Sunyoung , Kim Kyung Eun , Park Hyun Jeong , Cho Daeho
Published: April 2020

Erythroid differentiation regulator 1 (ERDR1) was newly identified as a secreted protein that plays an essential role in maintaining cell growth homeostasis. ERDR1 enhances apoptosis at high cell densities, leading to the inhibition of cell survival. Exogenous ERDR1 treatment decreases cancer cell proliferation and tumor growth as a result of increased apoptosis via the regulation of apoptosis-related gene expression. Moreover, ERDR1 plays a pivotal role in skin diseases; ERDR1 expression in actinic keratosis (AK) is negatively correlated with the increase in apoptosis. Because of its high specificity and efficiency, photodynamic therapy (PDT) is a common therapy for patients with various skin diseases, including cancer. Many studies indicate that apoptosis is mainly induced by PDT treatment. As an apoptosis inducer, the recovery of the ERDR1 expression after PDT is correlated with good therapeutic outcomes. Here, we review recent findings that highlight the function of ERDR1 in the control of apoptosis. Thus, ERDR1 may have a role in the apoptosis regulation of target cells in the lesions, as the recovery of its expression after PDT is correlated with good therapeutic outcomes.
Anti-Inflammatory Drugs as Anticancer Agents
Authors: Zappavigna Silvia , Cossu Alessia Maria , Grimaldi Anna , Bocchetti Marco , Ferraro Giuseppe Andrea , Nicoletti Giovanni Francesco , Filosa Rosanna , Caraglia Michele
Published: April 2020

Inflammation is strictly associated with cancer and plays a key role in tumor development and progression. Several epidemiological studies have demonstrated that inflammation can predispose to tumors, therefore targeting inflammation and the molecules involved in the inflammatory process could represent a good strategy for cancer prevention and therapy. In the past, several clinical studies have demonstrated that many anti-inflammatory agents, including non-steroidal anti-inflammatory drugs (NSAIDs), are able to interfere with the tumor microenvironment by reducing cell migration and increasing apoptosis and chemo-sensitivity. This review focuses on the link between inflammation and cancer by describing the anti-inflammatory agents used in cancer therapy, and their mechanisms of action, emphasizing the use of novel anti-inflammatory agents with significant anticancer activity.
The Epigenetic Profile of Tumor Endothelial Cells. Effects of Combined Therapy with Antiangiogenic and Epigenetic Drugs on Cancer Progression
Authors: Ciesielski Oskar , Biesiekierska Marta , Panthu Baptiste , Vialichka Varvara , Pirola Luciano , Balcerczyk Aneta
Published: April 2020

Tumors require a constant supply of nutrients to grow which are provided through tumor blood vessels. To metastasize, tumors need a route to enter circulation, that route is also provided by tumor blood vessels. Thus, angiogenesis is necessary for both tumor progression and metastasis. Angiogenesis is tightly regulated by a balance of angiogenic and antiangiogenic factors. Angiogenic factors of the vascular endothelial growth factor (VEGF) family lead to the activation of endothelial cells, proliferation, and neovascularization. Significant VEGF-A upregulation is commonly observed in cancer cells, also due to hypoxic conditions, and activates endothelial cells (ECs) by paracrine signaling stimulating cell migration and proliferation, resulting in tumor-dependent angiogenesis. Conversely, antiangiogenic factors inhibit angiogenesis by suppressing ECs activation. One of the best-known anti-angiogenic factors is thrombospondin-1 (TSP-1). In pathological angiogenesis, the balance shifts towards the proangiogenic factors and an angiogenic switch that promotes tumor angiogenesis. Here, we review the current literature supporting the notion of the existence of two different endothelial lineages: normal endothelial cells (NECs), representing the physiological form of vascular endothelium, and tumor endothelial cells (TECs), which are strongly promoted by the tumor microenvironment and are biologically different from NECs. The angiogenic switch would be also important for the explanation of the differences between NECs and TECs, as angiogenic factors, cytokines and growth factors secreted into the tumor microenvironment may cause genetic instability. In this review, we focus on the epigenetic differences between the two endothelial lineages, which provide a possible window for pharmacological targeting of TECs.
DDX6 Helicase Behavior and Protein Partners in Human Adipose Tissue-Derived Stem Cells during Early Adipogenesis and Osteogenesis
Authors: Marcon Bruna Hilzendeger , Rebelatto Carmen K. , Cofré Axel R. , Dallagiovanna Bruno , Correa Alejandro
Published: April 2020

DDX6 helicase is an RNA-binding protein involved in different aspects of gene expression regulation. The roles played by DDX6 depend on the complexes associated with it. Here, for the first time, we characterize the protein complexes associated with DDX6 in human adipose tissue-derived stem cells (hASCs) and analyze the dynamics of this helicase under different conditions of translational activity and differentiation. The results obtained demonstrated that the DDX6 helicase is associated with proteins involved in the control of mRNA localization, translation and metabolism in hASCs. DDX6 complexes may also assemble into more complex structures, such as RNA-dependent granules, the abundance and composition of which change upon inhibited translational activity. This finding supports the supposition that DDX6 is possibly involved in the regulation of the mRNA life cycle in hASCs. Although there was no significant variation in the protein composition of these complexes during early adipogenic or osteogenic induction, there was a change in the distribution pattern of DDX6: the number of DDX6 granules per cell was reduced during adipogenesis and was enhanced during osteogenesis.
Gut Microbial Catabolites of Tryptophan Are Ligands and Agonists of the Aryl Hydrocarbon Receptor: A Detailed Characterization
Authors: Vyhlídalová Barbora , Krasulová Kristýna , Pečinková Petra , Marcalíková Adéla , Vrzal Radim , Zemánková Lenka , Vančo Jan , Trávníček Zdeněk , Vondráček Jan , Karasová Martina , Mani Sridhar , Dvořák Zdeněk
Published: April 2020

We examined the effects of gut microbial catabolites of tryptophan on the aryl hydrocarbon receptor (AhR). Using a reporter gene assay, we show that all studied catabolites are low-potency agonists of human AhR. The efficacy of catabolites differed substantially, comprising agonists with no or low (i3-propionate, i3-acetate, i3-lactate, i3-aldehyde), medium (i3-ethanol, i3-acrylate, skatole, tryptamine), and high (indole, i3-acetamide, i3-pyruvate) efficacies. We displayed ligand-selective antagonist activities by i3-pyruvate, i3-aldehyde, indole, skatole, and tryptamine. Ligand binding assay identified low affinity (skatole, i3-pyruvate, and i3-acetamide) and very low affinity (i3-acrylate, i3-ethanol, indole) ligands of the murine AhR. Indole, skatole, tryptamine, i3-pyruvate, i3-acrylate, and i3-acetamide induced CYP1A1 mRNA in intestinal LS180 and HT-29 cells, but not in the AhR-knockout HT-29 variant. We observed a similar CYP1A1 induction pattern in primary human hepatocytes. The most AhR-active catabolites (indole, skatole, tryptamine, i3-pyruvate, i3-acrylate, i3-acetamide) elicited nuclear translocation of the AhR, followed by a formation of AhR-ARNT heterodimer and enhanced binding of the AhR to the CYP1A1 gene promoter. Collectively, we comprehensively characterized the interactions of gut microbial tryptophan catabolites with the AhR, which may expand the current understanding of their potential roles in intestinal health and disease.
Synthesis and Cytotoxic Activity Evaluation of New Cu(I) Complexes of Bis(pyrazol-1-yl) Acetate Ligands Functionalized with an NMDA Receptor Antagonist
Authors: Pellei Maura , Bagnarelli Luca , Luciani Lorenzo , Del Bello Fabio , Giorgioni Gianfabio , Piergentili Alessandro , Quaglia Wilma , De Franco Michele , Gandin Valentina , Marzano Cristina , Santini Carlo
Published: April 2020

In the present article, copper(I) complexes of bis(pyrazol-1-yl) carboxylic acid (LH), bis(3,5-dimethylpyrazol-1-yl) carboxylic acid (L2H), and bis(pyrazol-1-yl) acetates conjugated with an N-methyl-d-aspartate (NMDA) receptor antagonist (LNMDA or L2NMDA) and phosphane ligands (triphenylphosphine or 1,3,5-triaza-7-phosphaadamantane) were synthesized. The selection of an NMDA antagonist for the coupling with LH and L2H was suggested by the observation that NMDA receptors are expressed and play a role in different types of cancer models. All the new complexes showed a significant antitumor activity on a panel of human tumor cell lines of different histology, with cisplatin-sensitive, cisplatin-resistant, or multi-drug-resistant phenotype. Their half maximal inhibitory concentration (IC50) values were in the low- and sub-micromolar range and, in general, significantly lower than that of cisplatin. Interestingly, the fact that all the complexes proved to be significantly more active than cisplatin even in three-dimensional (3D) spheroids of H157 and BxPC3 cancer cells increased the relevance of the in vitro results. Finally, morphological analysis revealed that the most representative complex 8 induced a massive swelling of the endoplasmic reticulum (ER) membrane, which is a clear sign of ER stress.
mtDNA and Mitochondrial Stress Signaling in Human Diseases: A Special Issue
Authors: Pesce Vito , Lezza Angela Maria Serena
Published: April 2020

The completion of the Special Issue dedicated to “mtDNA and mitochondrial stress signaling in human diseases” requests a final overall look to highlight the most valuable findings among the many presented data [...]
Progestins Related to Progesterone and Testosterone Elicit Divergent Human Endometrial Transcriptomes and Biofunctions
Authors: Houshdaran Sahar , Chen Joseph C. , Vallvé-Juanico Júlia , Balayan Shayna , Vo Kim Chi , Smith-McCune Karen , Greenblatt Ruth M. , Irwin Juan C. , Giudice Linda C.
Published: April 2020

Progestins are widely used for the treatment of gynecologic disorders and alone, or combined with an estrogen, are used as contraceptives. While their potencies, efficacies and side effects vary due to differences in structures, doses and routes of administration, little is known about their effects on the endometrial transcriptome in the presence or absence of estrogen. Herein, we assessed the transcriptome and pathways induced by progesterone (P4) and the three most commonly used synthetic progestins, medroxyprogesterone acetate (MPA), levonorgestrel (LNG), and norethindrone acetate (NETA), on human endometrial stromal fibroblasts (eSF), key players in endometrial physiology and reproductive success. While there were similar transcriptional responses, each progestin induced unique genes and biofunctions, consistent with their structural similarities to progesterone (P4 and MPA) or testosterone (LNG and NETA), involving cellular proliferation, migration and invasion. Addition of estradiol (E2) to each progestin influenced the number of differentially expressed genes and biofunctions in P4 and MPA, while LNG and NETA signatures were more independent of E2. Together, these data suggest different mechanisms of action for different progestins, with progestin-specific altered signatures when combined with E2. Further investigation is warranted for a personalized approach in different gynecologic disorders, for contraception, and minimizing side effects associated with their use.
Right Ventricular Function in Chronic Heart Failure: From the Diagnosis to the Therapeutic Approach
Authors: Monitillo Francesco , Di Terlizzi Vito , Gioia Margherita Ilaria , Barone Roberta , Grande Dario , Parisi Giuseppe , Brunetti Natale Daniele , Iacoviello Massimo
Published: April 2020

There is growing attention for the study of the right ventricle in cardiovascular disease and in particular in heart failure. In this clinical setting, right ventricle dysfunction is a significant marker of poor prognosis, regardless of the degree of left ventricular dysfunction. Novel echocardiographic methods allow for obtaining a more complete evaluation of the right ventricle anatomy and function as well as of the related abnormalities in filling pressures. Specific and effective therapies for the right ventricle dysfunction are still not well defined and this represents the most difficult and important challenge. This article focuses on available diagnostic techniques for studying right ventricle dysfunction as well as on the therapies for right ventricle dysfunction.
On the Genesis of Artifacts in Neutron Transmission Imaging of Hydrogenous Steel Specimens
Authors: Pfretzschner Beate , Schaupp Thomas , Hannemann Andreas , Schulz Michael , Griesche Axel
Published: April 2020

Hydrogen-charged supermartensitic steel samples were used to systematically investigate imaging artifacts in neutron radiography. Cadmium stencils were placed around the samples to shield the scintillator from excessive neutron radiation and to investigate the influence of the backlight effect. The contribution of scattered neutrons to the total detected intensity was investigated by additionally varying the sample-detector distance and applying a functional correlation between distance and intensity. Furthermore, the influence of the surface roughness on the edge effect due to refraction was investigated.
A Comparison of Beach Nourishment Methodology and Performance at Two Fringing Reef Beaches in Waikiki (Hawaii, USA) and Cadiz (SW Spain)
Authors: Muñoz-Perez Juan J. , Gallop Shari L. , Moreno Luis J.
Published: April 2020

Fringing reefs have significant impacts on beach dynamics, yet there is little research on how they should be considered in beach nourishment design, monitoring, and conservation works. Thus, the behavior and characteristics of nourishment projects at two reef protected beaches, Royal Hawaiian Beach (RHB) in Hawaii, USA, and Victoria Beach (VB) in Cadiz, Spain, are compared to provide transferable information for future nourishment projects and monitoring in fringing reef environments. The nourishment cost at RHB was nine times higher than VB. This is partly due to lower total volume and a more complex placement and spreading method at RHB, despite the much closer borrow site at RHB. There was a significant difference in post-nourishment monitoring frequency and assessment of accuracy. RHB elevation was monitored quarterly for 2.7 years at 30 m-spaced profiles, compared to 5 years of biannual surveys of 50 m-spacing at VB. An additional problem related to the presence of reefs at both RHB and VB was estimating the beach volume increase after nourishment, due to variable definitions of the ‘beach’ area and high alongshore variability in reef topography. At sites where non-native sediment is used, it is imperative to understand how wave and current energy changes due to reefs will influence nourishment longevity. Thus, differences in erosion and accretion mechanisms at both beaches have been detected, though are still little understood. Moreover, discrepancies in sediment porosity between the two sites (which should be surveyed in future nourishments) have been found, probably due to differences in the nourishment sand transportation and distribution methods. In summary, more dialogue is needed to explicitly consider the influence of fringing reefs on coastal processes and beach nourishment projects.
Optimal Phase Load Balancing in Low Voltage Distribution Networks Using a Smart Meter Data-Based Algorithm
Authors: Grigoraș Gheorghe , Neagu Bogdan-Constantin , Gavrilaș Mihai , Triștiu Ion , Bulac Constantin
Published: April 2020

In the electric distribution systems, the “Smart Grid” concept is implemented to encourage energy savings and integration of the innovative technologies, helping the distribution network operators (DNOs) in choosing the investment plans which lead to the optimal operation of the networks and increasing the energy efficiency. In this context, a new phase load balancing algorithm was proposed to be implemented in the low voltage distribution networks with hybrid structures of the consumption points (switchable and non-switchable consumers). It can work in both operation modes (real-time and off-line), uploading information from different databases of the DNO which contain: The consumers’ characteristics, the real loads of the consumers integrated into the smart metering system (SMS), and the typical load profiles for the consumers non-integrated in the SMS. The algorithm was tested in a real network, having a hybrid structure of the consumption points, on a by 24-h interval. The obtained results were analyzed and compared with other algorithms from the heuristic (minimum count of loads adjustment algorithm) and the metaheuristic (particle swarm optimization and genetic algorithms) categories. The best performances were provided by the proposed algorithm, such that the unbalance coefficient had the smallest value (1.0017). The phase load balancing led to the following technical effects: decrease of the average current in the neutral conductor and the energy losses with 94%, respectively 61.75%, and increase of the minimum value of the phase voltage at the farthest pillar with 7.14%, compared to the unbalanced case.
Genome Mining as New Challenge in Natural Products Discovery
Authors: Albarano Luisa , Esposito Roberta , Ruocco Nadia , Costantini Maria
Published: April 2020

Drug discovery is based on bioactivity screening of natural sources, traditionally represented by bacteria fungi and plants. Bioactive natural products and their secondary metabolites have represented the main source for new therapeutic agents, used as drug leads for new antibiotics and anticancer agents. After the discovery of the first biosynthetic genes in the last decades, the researchers had in their hands the tool to understand the biosynthetic logic and genetic basis leading to the production of these compounds. Furthermore, in the genomic era, in which the number of available genomes is increasing, genome mining joined to synthetic biology are offering a significant help in drug discovery. In the present review we discuss the importance of genome mining and synthetic biology approaches to identify new natural products, also underlining considering the possible advantages and disadvantages of this technique. Moreover, we debate the associated techniques that can be applied following to genome mining for validation of data. Finally, we review on the literature describing all novel natural drugs isolated from bacteria, fungi, and other living organisms, not only from the marine environment, by a genome-mining approach, focusing on the literature available in the last ten years.
The Phylum Bryozoa: From Biology to Biomedical Potential
Authors: Ciavatta Maria Letizia , Lefranc Florence , Vieira Leandro M. , Kiss Robert , Carbone Marianna , van Otterlo Willem A. L. , Lopanik Nicole B. , Waeschenbach Andrea
Published: April 2020

Less than one percent of marine natural products characterized since 1963 have been obtained from the phylum Bryozoa which, therefore, still represents a huge reservoir for the discovery of bioactive metabolites with its ~6000 described species. The current review is designed to highlight how bryozoans use sophisticated chemical defenses against their numerous predators and competitors, and which can be harbored for medicinal uses. This review collates all currently available chemoecological data about bryozoans and lists potential applications/benefits for human health. The core of the current review relates to the potential of bryozoan metabolites in human diseases with particular attention to viral, brain, and parasitic diseases. It additionally weighs the pros and cons of total syntheses of some bryozoan metabolites versus the synthesis of non-natural analogues, and explores the hopes put into the development of biotechnological approaches to provide sustainable amounts of bryozoan metabolites without harming the natural environment.