bluuebunny/crossref_metadata_2025 huggingface dataset:
Equol and Resveratrol Improve Bone Turnover Biomarkers in Postmenopausal Women: A Clinical Trial
Authors: Corbi Graziamaria , Nobile Vincenzo , Conti Valeria , Cannavo Alessandro , Sorrenti Vincenzo , Medoro Alessandro , Scapagnini Giovanni , Davinelli Sergio
Published: July 2023

Estrogen deficiency is a major cause of loss of postmenopausal bone mineral density (BMD). This study aimed to evaluate the effects of equol and resveratrol on bone turnover biomarkers in postmenopausal women. Sixty healthy postmenopausal women were randomly assigned to receive 200 mg fermented soy containing 10 mg equol and 25 mg resveratrol or a placebo for 12 months. Whole-body BMD and bone turnover biomarkers, such as deoxypyridinoline (DPD), tartrate-resistant acid phosphatase 5b (TRACP-5b), osteocalcin, and bone-specific alkaline phosphatase (BAP), were measured at baseline and after 12 months of treatment. At the end of treatment, DPD, osteocalcin, and BAP significantly improved in the active group (p < 0.0001 for all) compared to the placebo group. Conversely, TRACP-5b levels were unaffected by supplementation (p = 0.051). Statistically significant changes in the concentrations of DPD (p < 0.0001), osteocalcin (p = 0.0001), and BAP (p < 0.0001) compared to baseline were also identified. Overall, the intervention significantly increased BMD measured in the whole body (p = 0.0220) compared with the placebo. These data indicate that the combination of equol and resveratrol may positively modulate bone turnover biomarkers and BMD, representing a potential approach to prevent age-related bone loss in postmenopausal women.
Dibenzo[b,f]oxepine Molecules Used in Biological Systems and Medicine
Authors: Krawczyk Hanna
Published: July 2023

In this short review, including 113 references, issues related to dibenzo[b,f]oxepine derivatives are presented. Dibenzo[b,f]oxepine scaffold is an important framework in medicinal chemistry, and its derivatives occur in several medicinally relevant plants. At the same time, the structure, production, and therapeutic effects of dibenzo[b,f]oxepines have not been extensively discussed thus far and are presented in this review. This manuscript addresses the following issues: extracting dibenzo[b,f]oxepines from plants and its significance in medicine, the biosynthesis of dibenzo[b,f]oxepines, the active synthetic dibenzo[b,f]oxepine derivatives, the potential of dibenzo[b,f]oxepines as microtubule inhibitors, and perspective for applications of dibenzo[b,f]oxepine derivatives. In conclusion, this review describes studies on various structural features and pharmacological actions of dibenzo[b,f]oxepine derivatives.
Adaptation of the Th-MYCN Mouse Model of Neuroblastoma for Evaluation of Disseminated Disease
Authors: Rahavi Seyed M. , Aletaha Maryam , Farrokhi Ali , Lorentzian Amanda , Lange Philipp F. , Maxwell Christopher A. , Lim Chinten James , Reid Gregor S. D.
Published: July 2023

High-risk neuroblastoma remains a profound clinical challenge that requires eradication of neuroblastoma cells from a variety of organ sites, including bone marrow, liver, and CNS, to achieve a cure. While preclinical modeling is a powerful tool for the development of novel cancer therapies, the lack of widely available models of metastatic neuroblastoma represents a significant barrier to the development of effective treatment strategies. To address this need, we report a novel luciferase-expressing derivative of the widely used Th-MYCN mouse. While our model recapitulates the non-metastatic neuroblastoma development seen in the parental transgenic strain, transplantation of primary tumor cells from disease-bearing mice enables longitudinal monitoring of neuroblastoma growth at distinct sites in immune-deficient or immune-competent recipients. The transplanted tumors retain GD2 expression through many rounds of serial transplantation and are sensitive to GD2-targeted immune therapy. With more diverse tissue localization than is seen with human cell line-derived xenografts, this novel model for high-risk neuroblastoma could contribute to the optimization of immune-based treatments for this deadly disease.
Predicting the Effect of Single Mutations on Protein Stability and Binding with Respect to Types of Mutations
Authors: Pandey Preeti , Panday Shailesh Kumar , Rimal Prawin , Ancona Nicolas , Alexov Emil
Published: July 2023

The development of methods and algorithms to predict the effect of mutations on protein stability, protein–protein interaction, and protein–DNA/RNA binding is necessitated by the needs of protein engineering and for understanding the molecular mechanism of disease-causing variants. The vast majority of the leading methods require a database of experimentally measured folding and binding free energy changes for training. These databases are collections of experimental data taken from scientific investigations typically aimed at probing the role of particular residues on the above-mentioned thermodynamic characteristics, i.e., the mutations are not introduced at random and do not necessarily represent mutations originating from single nucleotide variants (SNV). Thus, the reported performance of the leading algorithms assessed on these databases or other limited cases may not be applicable for predicting the effect of SNVs seen in the human population. Indeed, we demonstrate that the SNVs and non-SNVs are not equally presented in the corresponding databases, and the distribution of the free energy changes is not the same. It is shown that the Pearson correlation coefficients (PCCs) of folding and binding free energy changes obtained in cases involving SNVs are smaller than for non-SNVs, indicating that caution should be used in applying them to reveal the effect of human SNVs. Furthermore, it is demonstrated that some methods are sensitive to the chemical nature of the mutations, resulting in PCCs that differ by a factor of four across chemically different mutations. All methods are found to underestimate the energy changes by roughly a factor of 2.
Salivary Alterations of Myeloperoxidase in Patients with Systemic Diseases: A Systematic Review
Authors: Nijakowski Kacper , Jankowski Jakub , Gruszczyński Dawid , Surdacka Anna
Published: July 2023

Salivary myeloperoxidase (MPO) is a key mediator of the oral immune system, acting as an enzyme that utilises H2O2 to generate molecules with high bactericidal activity. While MPO determination in plasma is quite common, the use of saliva is still rare. Our systematic review was designed to answer the question “Are salivary levels of myeloperoxidase altered in patients with systemic diseases?”. Following the inclusion and exclusion criteria, we included twenty-six studies. Altered MPO levels in saliva were most commonly found in patients with cardiovascular and gastrointestinal diseases. Most studies concerned unstimulated whole saliva, and only a few of them stimulated, mainly by chewing paraffin. Enzyme-linked immunosorbent assay (ELISA) was the most common method for determination of MPO concentrations in saliva. Increased salivary MPO levels were more often observed for inflammatory diseases, except patients with inflammatory bowel diseases who were eligible for biologic therapy. In conclusion, MPO could be altered in the saliva of patients with systematic diseases, especially cardiovascular or gastrointestinal diseases. However, further investigations are recommended to validate these outcomes.
Biological Activities of Ceratonia siliqua Pod and Seed Extracts: A Comparative Analysis of Two Cretan Cultivars
Authors: Kavvoura Dafni-Alexandra , Stefanakis Michalis K. , Kletsas Dimitris , Katerinopoulos Haralambos E. , Pratsinis Harris
Published: July 2023

Ceratonia siliqua L., commonly known as the carob tree, appears in most Mediterranean countries, often cultivated for the collection of its fruits to be used as food for humans and animals. This study was aimed at the phytochemical characterization of two common Cretan C. siliqua cultivars and the biological evaluation of deseeded pod and seed extracts regarding their putative use in cosmetics. Gas and liquid chromatographic techniques were used to assess their essential oil, fatty acid, and carbohydrate profiles. Cell-free assays, including free-radical scavenging; the inhibition of tyrosinase and collagenase; the blocking of advanced glycation end product (AGE) formation; along with assays in human skin fibroblast cultures, i.e., reactive oxygen species suppression, glutathione stimulation, and protection from oxidative stress and from ultraviolet (UVB) radiation, were also used. Extracts from both cultivars were found to possess antioxidant capacity, tyrosinase- and collagenase-inhibitory activities, an ability to block glucose-induced AGEs, and in certain cases, UVB absorbance and photoprotective activities. Seed extracts were in general more active, while the use of 30% aqueous methanol seemed to be more efficient than n-hexane for extraction. Serial partition of the most active extracts resulted in fractions with enriched biological activities. These properties make Cretan carob extracts and their fractions suitable candidates for use in cosmetics.
Angiotensin Regulation of Vascular Homeostasis: Exploring the Role of ROS and RAS Blockers
Authors: Koumallos Nikolaos , Sigala Evangelia , Milas Theodoros , Baikoussis Nikolaos G. , Aragiannis Dimitrios , Sideris Skevos , Tsioufis Konstantinos
Published: July 2023

Extensive research has been conducted to elucidate and substantiate the crucial role of the Renin-Angiotensin System (RAS) in the pathogenesis of hypertension, cardiovascular disorders, and renal diseases. Furthermore, the role of oxidative stress in maintaining vascular balance has been well established. It has been observed that many of the cellular effects induced by Angiotensin II (Ang II) are facilitated by reactive oxygen species (ROS) produced by nicotinamide adenine dinucleotide phosphate (NADPH) oxidase. In this paper, we present a comprehensive overview of the role of ROS in the physiology of human blood vessels, specifically focusing on its interaction with RAS. Moreover, we delve into the mechanisms by which clinical interventions targeting RAS influence redox signaling in the vascular wall.
Resveratrol-Loaded Attalea funifera Oil Organogel Nanoparticles: A Potential Nanocarrier against A375 Human Melanoma Cells
Authors: Dourado Douglas , Batista Fabiana Pacheco Reis , Philadelpho Biane Oliveira , de Souza Myla Lôbo , de Cerqueira e Silva Mariana Barros , de Grandis Rone Aparecido , Miranda Priscila Anjos , Colauto Nelson Barros , Pereira Daniel T. , Formiga Fabio Rocha , Cilli Eduardo Maffud , Pavan Fernando Rogério , Oliveira de Souza Carolina , Ferreira Ederlan de Souza
Published: July 2023

This study aimed to evaluate Attalea funifera seed oil with or without resveratrol entrapped in organogel nanoparticles in vitro against A375 human melanoma tumor cells. Organogel nanoparticles with seed oil (SON) or with resveratrol entrapped in the seed oil (RSON) formed functional organogel nanoparticles that showed a particle size <100 nm, polydispersity index <0.3, negative zeta potential, and maintenance of electrical conductivity. The resveratrol entrapment efficiency in RSON was 99 ± 1%. The seed oil and SON showed no cytotoxicity against human non-tumor cells or tumor cells. Resveratrol at 50 μg/mL was cytotoxic for non-tumor cells, and was cytotoxic for tumor cells at 25 μg/mL. Resveratrol entrapped in RSON showed a decrease in cytotoxicity against non-tumor cells and cytotoxic against tumor cells at 50 μg/mL. Thus, SON is a potential new platform for the delivery of resveratrol with selective cytotoxic activity in the treatment of melanoma.
Ethics and Trustworthiness of AI for Predicting the Risk of Recidivism: A Systematic Literature Review
Authors: Farayola Michael Mayowa , Tal Irina , Connolly Regina , Saber Takfarinas , Bendechache Malika
Published: July 2023

Artificial Intelligence (AI) can be very beneficial in the criminal justice system for predicting the risk of recidivism. AI provides unrivalled high computing power, speed, and accuracy; all harnessed to strengthen the efficiency in predicting convicted individuals who may be on the verge of recommitting a crime. The application of AI models for predicting recidivism has brought positive effects by minimizing the possible re-occurrence of crime. However, the question remains of whether criminal justice system stakeholders can trust AI systems regarding fairness, transparency, privacy and data protection, consistency, societal well-being, and accountability when predicting convicted individuals’ possible risk of recidivism. These are all requirements for a trustworthy AI. This paper conducted a systematic literature review examining trust and the different requirements for trustworthy AI applied to predicting the risks of recidivism. Based on this review, we identified current challenges and future directions regarding applying AI models to predict the risk of recidivism. In addition, this paper provides a comprehensive framework of trustworthy AI for predicting the risk of recidivism.
Gut Bacterial Diversity of Insecticide-Susceptible and Insecticide-Resistant Megalurothrips usitatus (Thysanoptera: Thripidae) and Elucidation of Their Putative Functional Roles
Authors: Zhu Bifeng , Chen Yueyin , Zhou Chenyan , Li Haolong , Ali Shaukat , Wu Jianhui
Published: July 2023

The gut bacterial microbiota of insects plays a crucial role in physiological, metabolic, and innate immune processes. In the current study, the gut bacterial communities of an insecticide-susceptible (IS), and a resistant (IR) population of a major legume pest, Megalurothrips usitatus (Bagnall), were evaluated. The 16S rDNA V3 + V4 regions of M. usitatus infected with Beauveria brongniartii along with the intestinal flora of both populations were sequenced based on a High-throughput sequencing platform. Toxicological bioassays revealed that the IR population exhibited resistance to acetamiprid and B. brongniartii isolate SB010 at levels of 138.0-fold and 55.6-fold higher, respectively, compared to the IS population. Through 16S High-throughput sequencing, the results indicate that both resistant populations, as well as B. brongniartii infestation, reduce the number of species of M. usitatus gut microbes. Using KEGG function prediction, it was found that most intestinal bacteria were involved in various metabolic activities, and the abundance of resistant populations was higher than that of sensitive populations. The bacteria in the gut of M. usitatus are mainly involved in various metabolic activities to achieve the degradation of B. brongniartii. This study provides valuable insights into the interaction between gut bacteria, insecticide resistance, and Beauveria. brongniartii infection in Megalurothrips usitatus, which can help inform future pest control strategies.
Saliva-Mediated Contrasting Effects of Two Citrus Aphid Species on Asian Citrus Psyllid Feeding Behavior and Plant Jasmonic Acid Pathway
Authors: Gao Jing , Tao Tonglai , Arthurs Steven P. , Hussain Mubasher , Ye Fengxian , Mao Runqian
Published: July 2023

While herbivorous insect saliva plays a crucial role in the interaction between plants and insects, its role in the inter-specific interactions between herbivorous insects has received little attention. Pre-infestation of citrus plants with Aphis spiraecola Patch and Aphis (Toxoptera) citricidus (Kirkaldy) exhibited positive and negative effects on the performance (feeding and reproduction) of Diaphorina citri Kuwayama. We explored the role of saliva in this plant-mediated interaction by infiltrating fresh and boiled aphid saliva into plants and detecting D. citri feeding behavior and citrus plant defense response. Leaf infiltration of A. spiraecola saliva disrupted the subsequent feeding of D. citri, indicated by prolonged extracellular stylet pathway duration and decreased phloem sap ingestion duration. By contrast, infiltration of A. citricidus saliva decreased the duration of the extracellular stylet pathway and phloem sap ingestion of D. citri. Furthermore, gene expression analysis showed that several salicylic acid (SA)- and jasmonic acid (JA)-pathway-related genes were activated by A. spiraecola saliva infiltration. However, two SA-pathway-related genes were activated and three JA-pathway-related genes were suppressed following A. citricidus saliva infiltration. Treatment with boiled saliva did not similarly impact D. citri feeding behavior or plant defense response. This study suggests that salivary components (those that can be inactivated by heating) from two citrus aphid species differently affect plant defenses and that they were responsible for the contrasting plant-mediated effects of two citrus aphids on the feeding behavior of D. citri. This study indicates a novel three-way citrus aphid–plant–citrus psyllid interaction.
Intravenous Treprostinil in Severe Inoperable Chronic Thromboembolic Pulmonary Hypertension Using Implantable Pumps—Single-Center Experience over More Than a Decade
Authors: Steringer-Mascherbauer Regina , Huber Charlotte , Fröschl Uwe , Engleder Dagmar , Függer Reinhold , Lummersdorfer Maria , Lenhard Ralf , Martinek Martin
Published: July 2023

The management of chronic thromboembolic pulmonary hypertension has significantly changed over the last decade with the availability of both specific therapies and interventional treatments. In parallel, implantable pumps for intravenous administration of treprostinil have broadened the spectrum of continuous prostanoid infusion. We evaluated the course of 17 consecutive patients with inoperable chronic thromboembolic pulmonary hypertension treated with treprostinil by means of an implantable infusion pump between 2011 and 2023 at our center. Complications associated with the infusion system were rare, leading to 0.4 unplanned surgical interventions during 17,160 patient days. No additional safety signals were detected, and clinical benefits achieved with subcutaneous treprostinil before pump implantation could be maintained in all patients. No catheter-related infections or thromboembolic events were observed. Implantable infusion pumps offer an attractive alternative to subcutaneous treprostinil for patients intolerant to the subcutaneous route, including those with chronic thromboembolic pulmonary hypertension.
Cardiac Magnetic Resonance—Detected Acute Myocardial Edema as Predictor of Favourable Prognosis: A Comprehensive Review
Authors: Sinigiani Giulio , De Michieli Laura , De Conti Giorgio , Ricci Fabrizio , De Lazzari Manuel , Migliore Federico , Perazzolo Marra Martina , Zorzi Alessandro , Corrado Domenico , Cipriani Alberto
Published: July 2023

Acute myocardial edema (AME) is increased water content in the myocardium and represents the first and transient pathophysiological response to an acute myocardial injury. In-vivo and non-invasive evaluation is feasible with cardiac magnetic resonance (CMR), which is a powerful imaging technique capable of tissue characterization. In the clinical setting, early demonstration of AME has a recognized diagnostic value for acute coronary syndromes and acute myocarditis, although its prognostic value is not well established. This article provides a comprehensive narrative review on the clinical meaning of AME in heart diseases. In particular, the available evidence of a possible favourable prognostic value in several clinical scenarios is addressed.
Biodegradable Magnesium Alloys for Personalised Temporary Implants
Authors: Hendea Radu Emil , Raducanu Doina , Claver Adrián , García José Antonio , Cojocaru Vasile Danut , Nocivin Anna , Stanciu Doina , Serban Nicolae , Ivanescu Steliana , Trisca-Rusu Corneliu , Campian Radu Septimiu
Published: July 2023

The objective of this experimental work was to examine and characterise the route for obtaining demonstrative temporary biodegradable personalised implants from the Mg alloy Mg-10Zn-0.5Zr-0.8Ca (wt.%). This studied Mg alloy was obtained in its powder state using the mechanical alloying method, with shape and size characteristics suitable for ensuing 3D additive manufacturing using the SLM (selective laser melting) procedure. The SLM procedure was applied to various processing parameters. All obtained samples were characterised microstructurally (using XRD—X-ray diffraction, and SEM—scanning electron microscopy); mechanically, by applying a compression test; and, finally, from a corrosion resistance viewpoint. Using the optimal test processing parameters, a few demonstrative temporary implants of small dimensions were made via the SLM method. Our conclusion is that mechanical alloying combined with SLM processing has good potential to manage 3D additive manufacturing for personalised temporary biodegradable implants of magnesium alloys. The compression tests show results closer to those of human bones compared to other potential metallic alloys. The applied corrosion test shows result comparable with that of the commercial magnesium alloy ZK60.
Nighttime Image Dehazing by Render
Authors: Jin Zheyan , Feng Huajun , Xu Zhihai , Chen Yueting
Published: July 2023

Nighttime image dehazing presents unique challenges due to the unevenly distributed haze caused by the color change of artificial light sources. This results in multiple interferences, including atmospheric light, glow, and direct light, which make the complex scattering haze interference difficult to accurately distinguish and remove. Additionally, obtaining pairs of high-definition data for fog removal at night is a difficult task. These challenges make nighttime image dehazing a particularly challenging problem to solve. To address these challenges, we introduced the haze scattering formula to more accurately express the haze in three-dimensional space. We also proposed a novel data synthesis method using the latest CG textures and lumen lighting technology to build scenes where various hazes can be seen clearly through ray tracing. We converted the complex 3D scattering relationship transformation into a 2D image dataset to better learn the mapping from 3D haze to 2D haze. Additionally, we improved the existing neural network and established a night haze intensity evaluation label based on the idea of optical PSF. This allowed us to adjust the haze intensity of the rendered dataset according to the intensity of the real haze image and improve the accuracy of dehazing. Our experiments showed that our data construction and network improvement achieved better visual effects, objective indicators, and calculation speed.
Polymetallic Nodule Resource Assessment of Seabed Photography Based on Denoising Diffusion Probabilistic Models
Authors: Shao Mingyue , Song Wei , Zhao Xiaobing
Published: July 2023

Polymetallic nodules, found abundantly in deep-ocean deposits, possess significant economic value and represent a valuable resource due to their high metal enrichment, crucial for the high-tech industry. However, accurately evaluating these valuable mineral resources presents challenges for traditional image segmentation methods due to issues like color distortion, uneven illumination, and the diverse distribution of nodules in seabed images. Moreover, the scarcity of annotated images further compounds these challenges, impeding resource assessment efforts. To overcome these limitations, we propose a novel two-stage diffusion-based model for nodule image segmentation, along with a linear regression model for predicting nodule abundance based on the coverage obtained through nodule segmentation. In the first stage, we leverage a diffusion model trained on predominantly unlabeled mineral images to extract multiscale semantic features. Subsequently, we introduce an efficient segmentation network designed specifically for nodule segmentation. Experimental evaluations conducted on a comprehensive seabed nodule dataset demonstrate the exceptional performance of our approach compared to other deep learning methods, particularly in addressing challenging conditions like uneven illumination and dense nodule distributions. Our proposed model not only extends the application of diffusion models but also exhibits superior performance in seabed nodule segmentation. Additionally, we establish a linear regression model that accurately predicts nodule abundance by utilizing the coverage calculated through seabed nodule image segmentation. The results highlight the model’s capacity to accurately assess nodule coverage and abundance, even in regions beyond the sampled sites, thereby providing valuable insights for seabed resource evaluation.
Study of the Hull Structural Deformation Calculation Using the Matrix Displacement Method and Its Influence on the Shaft Alignment
Authors: Zhou Weixin , Zhao Yao , Yuan Hua , Wang Xiaoqiang
Published: July 2023

The analysis of the influence of hull deformation on shaft alignment is predominately conducted using the finite element method (FEM), which is time-consuming, labor-intensive, and challenging to use for iterative hull design optimization. In this paper, hull deformation is separated into two parts—global deformation and local deformation, simplified to a single-span beam model and a grillage beam model, respectively—then solved using the matrix displacement method (MDM). Compared to FEM, the proposed method has a small calculation error, proving its correctness, while the calculation time is greatly reduced. The proposed method has been used to calculate the hull deformation of a ship under various conditions and evaluate its influence on shaft alignment. The results indicate that under certain conditions, the bearing reaction forces are constant, whereas the bearing pressure changes as a consequence of the change in shaft-to-bearing angle. The comparison between local rotation and shaft-to-bearing angle reveals that bearings in various positions follow distinct laws. We suggest that the shaft-to-bearing angle be used as an additional parameter in the evaluation of shaft alignment calculations. Moreover, when optimizing bearing pressure, bearings in different positions are affected differently by global and local deformation, and their optimization priorities are distinct.
Investigation of Multi-Timescale Sea Level Variability near Jamaica in the Caribbean Using Satellite Altimetry Records
Authors: Maitland Deron O. , Taylor Michael A. , Stephenson Tannecia S.
Published: July 2023

There is a dearth of studies characterizing historical sea level variability at the local scale for the islands in the Caribbean. This is due to the lack of reliable long term tide gauge data. There is, however, a significant need for such studies given that small islands are under increasing threat from rising sea levels, storm surges, and coastal flooding due to global warming. The growing length of satellite altimetry records provides a useful alternative to undertake sea level analyses. Altimetry data, spanning 1993–2019, are used herein to explore multi-timescale sea level variability near the south coast of Jamaica, in the northwest Caribbean. Caribbean basin dynamics and largescale forcing mechanisms, which could account for the variability, are also investigated. The results show that the average annual amplitude off the south coast of Jamaica is approximately 10 cm with a seasonal peak during the summer (July–August). The highest annual sea levels occur within the Caribbean storm season, adding to the annual risk. The annual trend over the 27 years is 3.3 ± 0.4 mm/yr when adjusted for Glacial Isostatic Adjustment (GIA), instrumental drift, and accounting for uncertainties. This is comparable to mean global sea level rise, but almost twice the prior estimates for the Caribbean which used altimetry data up to 2010. This suggests an accelerated rate of rise in the Caribbean over the last decade. Empirical Orthogonal Function (EOF) and correlation analyses show the long-term trend to be a basin-wide characteristic and linked to warming Caribbean sea surface temperatures (SSTs) over the period. When the altimetry data are detrended and deseasoned, the leading EOF mode has maximum loadings over the northwest Caribbean, including Jamaica, and exhibits interannual variability which correlates significantly with a tropical Pacific-tropical Atlantic SST gradient index, local wind strength, and the Caribbean Low Level Jet (CLLJ). Correlations with the El Niño Southern Oscillation (ENSO) in summer, seen in this and other studies, likely arise through the contribution of the ENSO to the SST gradient index and the ENSO’s modulation of the CLLJ peak strength in July. The results demonstrate the usefulness of altimetry data for characterizing sea level risk on various timescales for small islands. They also suggest the potential for developing predictive models geared towards reducing those risks.
The Current and Future States of Diagnostic Tests for Histoplasmosis with a Focus on People with HIV and Disseminated Histoplasmosis
Authors: Villareal Kenneth , Price Austin , Pasqualotto Alessandro C. , Bahr Nathan C.
Published: July 2023

Histoplasmosis is caused by Histoplasma capsulatum and, although endemic in large parts of the world, is often underrecognized in many locations. In addition to underrecognition, inadequate availability of diagnostic tests is a major contributor to poor outcomes in disseminated disease in people with HIV. For those with advanced HIV and disseminated disease, antibody testing is less useful. Culture and histopathology can be useful in this situation, but each has limitations, including variable sensitivity by site and, in the case of culture, the need for a biosafety level three laboratory and a long period of growth. Antigen testing has proven useful for disseminated histoplasmosis due to the excellent sensitivity of urine. Yet, turnaround is slower than ideal due to use in a limited number of centers. The development of lateral flow assays has the potential to make for true rapid point-of-care assays for histoplasmosis, but in order to meet that promise, the tests must be widely available and affordable.
The Effect of Ustilago maydis and Delayed Harvesting on A- and B-Type Trichothecene Concentrations in Maize Grain
Authors: Venslovas Eimantas , Mankevičienė Audronė , Kochiieru Yuliia , Janavičienė Sigita , Dabkevičius Zenonas , Bartkevičs Vadims , Bērziņa Zane , Pavlenko Romans
Published: July 2023

The aim of this study was to investigate whether, in the context of a higher incidence of Ustilago maydis and Fusarium spp. at optimal and delayed harvest times, a higher incidence of mycotoxin contamination in maize grains could be expected. The field experiment was carried out at the Lithuanian Research Centre for Agriculture and Forestry experimental fields over three consecutive years (2020–2022). Two maize hybrids (Duxxbury and Lapriora) with different FAO numbers were used. The experimental design in the field was a randomized complete block design. Harvesting took place at three different times: first at physiological maturity, and then 10 (±2) and 20 (±2) days after the first harvest. Each hybrid had four repetitions at different harvest times. The U. maydis infection was only detected in 2021 and after the first harvest cobs were further divided into four different groups with four repetitions: healthy cobs, cobs visually infected with Fusarium spp., cobs visually infected with common smut, and cobs visually infected with both pathogens. No U. maydis-damaged maize cobs were found in 2020 and 2022. The levels of Fusarium microscopic fungi in maize grains were also from 4 to 16 times higher in 2021 than in 2020 and 2022. Harvest delays in 2020 led to a significant deoxynivalenol concentration increase in the Duxxbury hybrid and an HT-2 concentration increase in the Lapriora hybrid. In 2021, deoxynivalenol, 3-acetyl-deoxynivalenol, 15-acetyl-deoxynivalenol, and HT-2 concentrations significantly rose in both hybrids, but the T-2 concentration significantly increased only in the Lapriora hybrid. Deoxynivalenol concentrations were, respectively, 110 and 14.6 times higher than in cobs only infected with Fusarium spp. or U. maydis. Concentrations of 15-acetyl-deoxynivalenol were, respectively, 60, 67, and 43 times higher than in asymptomatic cobs and cobs only infected with Fusarium spp. or U. maydis. Cobs contaminated with both pathogens also had higher concentrations of 3-acetyl-deoxynivalenol. T-2 and HT-2 were detected in maize grains harvested from cobs infected only with Fusarium spp. The presence of U. maydis and Fusarium fungi in maize cobs, along with harvest delays, led to significant increases in mycotoxin concentrations, highlighting the importance of timely harvesting and pathogen management to mitigate mycotoxin contamination in maize grains.
Genotypic and Phenotypic Diversity of Kluyveromyces marxianus Isolates Obtained from the Elaboration Process of Two Traditional Mexican Alcoholic Beverages Derived from Agave: Pulque and Henequen (Agave fourcroydes) Mezcal
Authors: Lappe-Oliveras Patricia , Avitia Morena , Sánchez-Robledo Sara Darinka , Castillo-Plata Ana Karina , Pedraza Lorena , Baquerizo Guillermo , Le Borgne Sylvie
Published: July 2023

Seven Kluyveromyces marxianus isolates from the elaboration process of pulque and henequen mezcal were characterized. The isolates were identified based on the sequences of the D1/D2 domain of the 26S rRNA gene and the internal transcribed spacer (ITS-5.8S) region. Genetic differences were found between pulque and henequen mezcal isolates and within henequen mezcal isolates, as shown by different branching patterns in the ITS-5.8S phylogenetic tree and (GTG)5 microsatellite profiles, suggesting that the substrate and process selective conditions may give rise to different K. marxianus populations. All the isolates fermented and assimilated inulin and lactose and some henequen isolates could also assimilate xylose and cellobiose. Henequen isolates were more thermotolerant than pulque ones, which, in contrast, presented more tolerance to the cell wall-disturbing agent calcofluor white (CFW), suggesting that they had different cell wall structures. Additionally, depending on their origin, the isolates presented different maximum specific growth rate (µmax) patterns at different temperatures. Concerning tolerance to stress factors relevant for lignocellulosic hydrolysates fermentation, their tolerance limits were lower at 42 than 30 °C, except for glucose and furfural. Pulque isolates were less tolerant to ethanol, NaCl, and Cd. Finally, all the isolates could produce ethanol by simultaneous saccharification and fermentation (SSF) of a corncob hydrolysate under laboratory conditions at 42 °C.
Craniofacial Phenotype in Obstructive Sleep Apnea and Its Impact on Positive Airway Pressure (PAP) Adherence
Authors: Park Jae-Seon , Kwon Bin , Kang Hyun-Seok , Yun Seong-Jin , Han Sung-Jun , Choi Yeso , Kang Sung-Hun , Lee Mi-Yeon , Lee Kyung-Chul , Hong Seok-Jin
Published: July 2023

Positive airway pressure (PAP) is an important treatment tool for patients with moderate and severe obstructive sleep apnea (OSA), and adherence to PAP significantly affects treatment outcomes. Disease severity, adverse effects, and psychosocial factors are known to predict medication adherence. Cephalometric parameters have been reported to positively correlate with upper airway collapse. However, research on the correlation between these cephalometric parameters and PAP adherence remains insufficient. This study aimed to identify this relationship. This study included 185 patients with OSA who were prescribed PAP. Polysomnography (PSG) was performed to diagnose OSA, and paranasal sinus computed tomography (PNS CT) was performed to check for comorbidities of the upper airway. In addition, cephalometric parameters such as the hyoid–posterior nasal spine (H-PNS), posterior nasal spine–mandibular plane (PNS-MP), and hyoid–mandibular plane (H-MP) were measured in the midsagittal and axial CT views. Adherence was evaluated 3–12 months after the PAP prescription. A total of 136 patients were PAP-adherent, and 49 were nonadherent. There were more males in the adherent group and a higher average height in the adherent group. The PSG results showed that the apnea–hypopnea index (AHI), respiratory disturbance index (RDI), oxygen desaturation index (ODI), arousal index (AI), rapid eye movement (REM) AHI, and supine AHI were significantly higher, and the lowest oxygen saturation was lower in the adherent group. In the analysis of covariance (ANCOVA) model adjusted for sex and height, among the cephalometric parameters, H-MP was significantly longer in the adherent group (p = 0.027), and H-PNS showed a longer tendency (p = 0.074). In the logistic regression analysis model, the odds ratio (OR) and 95% confidence intervals (95% CI) of adherence and severe OSA in the third tertile compared to the first tertile of H-MP were 2.93 (1.25–6.86) and 4.00 (1.87–8.56). In the case of H-PNS, they were 2.58 (1.14–5.81) and 4.86 (2.24–10.54), respectively. This study concluded that an inferiorly placed hyoid bone in adult patients is associated with PAP adherence and disease severity.
Prescription Trends of Biologic DMARDs in Treating Rheumatologic Diseases: Changes of Medication Availability in COVID-19
Authors: Radić Mislav , Đogaš Hana , Vrkić Karla , Gelemanović Andrea , Marinović Ivanka , Perković Dijana , Nazlić Jurica , Radić Josipa , Krstulović Daniela Marasović , Meštrović Julije
Published: July 2023

Biologic disease-modifying antirheumatic drugs (DMARDs) are very effective in treating rheumatic diseases with a good patient tolerance. However, high cost and individualistic approach requires dedication of the physician. Therefore, the aim of this study was to determine how the COVID-19 pandemic has affected the prescription of biologic DMARDs in rheumatology at the University Hospital of Split. The data collection was conducted through an archive search in the Outpatient Clinic for Rheumatology in the University Hospital of Split, Split, Croatia. The search included the period before and after the start of the COVID-19 pandemic in Croatia (31 March 2020). Collected data included age, sex, ICD-10 code of diagnosis, generic and brand name of the prescribed drug, date of therapy initiation, and medication administration route. In the pre-COVID-19 period, 209 patients were processed, while in the COVID-19 period, 185 patients were processed (11.5% fewer). During pre-COVID-19, 231 biologic medications were prescribed, while during COVID-19, 204. During COVID-19, IL-6 inhibitors were less prescribed (48 (21%) vs. 21 (10%) prescriptions, p = 0.003), while IL-17A inhibitors were more prescribed (39 (17%) vs. 61 (30%) prescriptions, p = 0.001). In ankylosing spondylitis (AS), adalimumab was prescribed more during pre-COVID-19 (25 vs. 15 patients, p = 0.010), while ixekizumab was prescribed less (1 vs. 10 patients, p = 0.009). In rheumatoid arthritis, tocilizumab was prescribed more in the pre-COVID-19 period (34 vs. 10 patients, p = 0.012). Overall, the prescription trends of biologic DMARDs for rheumatologic diseases did not vary significantly in the University Hospital of Split, Croatia. Tocilizumab was prescribed less during COVID-19 due to shortages, while ixekizumab was more prescribed during COVID-19 due to an increase in psoriatic arthritis patients processed and due to being approved for treating AS.
The Six Decades of the Capital Asset Pricing Model: A Research Agenda
Authors: Kumar Santosh , Kumar Ankit , Singh Kamred Udham , Patra Sujit Kumar
Published: July 2023

This paper re-examines the presence of the Sharpe–Treynor–Lintner–Mossin capital asset pricing model (CAPM) in the finance literature and is accompanied by a bibliometric summary analysis. The popular model is in its sixth decade; we summarized the relevance of the CAPM using publication and citation trends, as well as identifying its most prolific and impactful contributors. This paper is based on a systematic review of the literature and was completed with the help of various bibliometric techniques. During the study process, we presented a map of various themes and areas of the CAPM and its evolution. Our findings indicate that the extant literature on this topic (the cost of capital, asset pricing, portfolio, risk management, beta, systematic risk, and value premium) is based on the principles and assumptions of the CAPM. We are considering suggestions on the future use, trend, and direction of the CAPM, based on our summary of thematically developed clusters.
International Borrowing and Lending in the Presence of Oligopolistic Competition
Authors: Kumar Ronald Ravinesh , Stauvermann Peter J.
Published: July 2023

This paper examines the implications of imperfect competition in a two-country framework where a single good is produced. Using an overlapping generation model, we analyze the effects of market structures. Specifically, one country is assumed to operate under a perfectly competitive market structure, while the other country operates under an oligopolistic market structure. Our analysis reveals that the differences in factor prices between the two countries when they are in autarky lead to intergenerational trade once their capital markets are integrated. A key finding is that the country with an oligopolistic market structure becomes a lending country, while the country with a competitive market structure becomes a borrowing country. Furthermore, we find that the country with an oligopolistic market structure, serving as a lender, experiences a current account surplus, while the country with a perfectly competitive market structure, acting as a debtor, incurs a current account deficit.
Fertilizer Application in Contract Farming: A Risk Analysis
Authors: Zhang Yiming , Yang Rui , Zhao Kai , Kong Xiangzhi
Published: July 2023

To our knowledge, this is the first study in the world to incorporate risk into the contract-farming participation decision process and to examine empirical evidence on the effect of contract farming on fertilizer application, filling the research gaps in the relevant literature and providing perspectives on both chemical fertilizer reduction and organic fertilizer application behavior. To estimate the impact of contract farming on farmers’ application of chemical fertilizer and organic fertilizer, we used data on tea farmers from the Fujian and Hubei provinces in China and the recursive binary probit model. The 2SLS (two-stage least-squares) model was used to estimate the impact of the contract-farming participation rate on farmers’ organic and chemical fertilizer application intensity. The empirical results show that farmers’ risk-prevention ability had a significant negative impact on farmers’ contract-farming participation decision and rates. Contract-farming participation had a significant, positive impact on farmers’ organic and chemical fertilizer application intensity, while contract-farming participation rates had a significant, negative impact on the intensity of chemical fertilizer application by farmers. However, contract-farming participation rates did not have a significant impact on organic and chemical fertilizer application intensity. To promote fertilizer reduction and organic fertilizer incremental application, an effective strategy could be to promote farmers’ participation in contract agriculture.
Transport Task Models with Variable Supplier Availabilities
Authors: Vasilev Julian , Nikolaev Rosen , Milkova Tanka
Published: July 2023

Background: With regard to the definition of an optimal transport plan for some material flow in the logistics system in literature and practice, the classical transport task model is developed. The minimization of total transport costs is usually considered for optimality. Some modifications to the classical transport task have also been developed. Methods: The article uses the methods of linear optimization. Based on these methods, two modified transport task models have been constructed, which consider the possibility of planning in advance the quantities available from the suppliers of the transported cargo. These models are applicable in SCM for pharmaceuticals with a national logistics hub. Furthermore, a solver in MS Excel is used to determine the optimal solution of optimization models. Results: Two new (modified, extended) models of the transport task have been constructed, in which a preliminary planning of the available quantities of the transported cargo at the suppliers is made. These quantities shall be planned in such a way as to ensure a minimum total transport cost. Conclusions: By applying the proposed new transport task models, lower total transport costs for carrying out imported pharmaceuticals can be ensured compared to an application of the classical transport task model.
Experimental Investigation on a Novel Temperature-Controlled Phase Change Aggregate Concrete: Thermo-Mechanical Properties and Hydration Heat Control
Authors: Wang Yejia , Wang Chengjin , Luo Aibo , Dong Minqi , Su Qian , Zhou Chenling , Zhang Zongyu , Pei Yanfei
Published: July 2023

To reduce the structural deterioration of mass concrete structures from temperature cracks, and lower energy consumption caused by the traditional mass concrete hydration heat cooling process, this paper reports the preparation of concrete temperature-controlled phase change aggregate (PCA) by a vacuum compaction method using light and high-strength black ceramite and No. 58 fully refined paraffin wax as phase change material (PCM), and the encapsulation technology of the aggregate by using superfine cement and epoxy resin. Further, through laboratory tests, the cylinder compressive strength, thermal stability and mixing breakage rate of the encapsulated PCA were tested, and the differences in mechanical properties such as compressive strength, flexural strength and splitting tensile strength between phase change aggregate concrete (PCAC) and ordinary concrete were studied. A test method was designed to test the heat storage effect of PCA, and the temperature control effect of PCAC was analyzed based on the law of conservation of energy. The research conclusions are as follows: (1) Both superfine cement and epoxy resin shells increase the strength of the aggregate, with the epoxy resin increasing it more than the superfine cement. The thermal stabilization of the PCA is good after encapsulation of superfine cement and epoxy resin. However, PCA encapsulated in superfine cement is more easily crushed than that encapsulated in epoxy resin. (2) Under the condition of water bath heating and semi-insulation, when the water bath temperature reaches 85 °C, the temperature difference between the PCA and the common stone aggregate can be up to 6 °C. Based on the law of energy conservation, the test results will be converted to mass concrete with the same volume of aggregate mixture;, the difference of PCAC and ordinary concrete temperature can be up to 10 °C, so the temperature control effect is significant. (3) The mechanical properties of PCAC with 100% aggregate replacement rate compared to ordinary concrete are reduced to varying degrees, and the performance decline of the epoxy-encapsulated PCA is smaller than that encapsulated with superfine cement; in an actual project, it is possible to improve the concrete grade to make up for this defect.