bluuebunny/crossref_metadata_2025 huggingface dataset:
Impacts of Particle Shape, Skeletal Porosity and Density on the Settling Velocity of Gravel‐Size Coral Debris
Authors: Mao Lilei , Li Jiabo , Shimozono Takenori , Tajima Yoshimitsu
Published: May 2023

Knowledge of the hydrodynamic behavior of carbonate bioclasts is essential for solving coastal morphodynamic problems in marine carbonate depositional environments. Marine carbonate grains are produced within the sedimentary system itself and are deformed by subsequent hydrodynamics, resulting in particle densities, sizes and shapes that are distinct from those of terrigenous sediments. In this study, we investigated the shapes and settling characteristics of gravel‐size natural coral debris collected from the coasts of Okinawa Prefecture, Japan. Based on the shape parameters and settling velocities obtained using image analysis techniques and settling tube experiments, respectively, a correlation model for predicting the settling velocity of coral gravel particles was developed by extending the previous formula of Dietrich (1982, https://doi.org/10.1029/wr018i006p01615) over particle Reynolds number values ranging from 384.23 to 4439.76. The model determines the settling velocity as a function of particle nominal diameter, form, roundness, and surface texture parameters that were selected after examining several other shape descriptors. Compared with other models, the proposed model showed better settling velocity predictive skills for coral gravel particles classified into four shape types. The proposed model also determines how the drag coefficient of coral gravel particles should be modified from that for spherical particles, by accounting for the abovementioned coral gravel particle shape parameters. The obtained data set of settling velocity and modification of the drag coefficient can be used for quantitative interpretation of suspension, transport, and sedimentation processes in marine carbonate depositional environments.
The Role of Atmospheric Rivers on Groundwater: Lessons Learned From an Extreme Wet Year
Authors: Siirila‐Woodburn Erica R. , Dennedy‐Frank P. James , Rhoades Alan , Vahmani Pouya , Maina Fadji , Hatchett Benjamin , Zhou Yang , Jones Andrew
Published: May 2023

In the coastal regions of the western United States, atmospheric rivers (ARs) are associated with the largest precipitation generating storms and contribute up to half of annual precipitation, but the impact of ARs on the integrated hydrologic cycle, specifically on groundwater storage and hydrodynamics, is largely unknown. To better explore the hydrologic behavior of AR versus non‐AR event precipitation, we present a novel combination of two water tracking methods (one in the atmosphere and one in the subsurface) to explicitly track the full lifecycle of water parcels generated by ARs. Simulations of northern California's Cosumnes River watershed during the record wet 2017 water year are performed via the coupling of a high‐resolution regional climate model and a land surface‐groundwater model accounting for lateral groundwater flow. Despite ARs contributing more precipitation than non‐AR storms, we find less AR water is preferentially stored in aquifers by year end. Fractionally, ARs result in 300% less snow derived groundwater‐recharged compared to non‐AR precipitation. Rain‐on‐snow (RoS) plays an important role in AR‐driven discharge, where over 50% of total discharge from ARs snow is from RoS events. Finally, despite record‐breaking annual precipitation, simulated groundwater depletion occurs by year end due to estimates of groundwater pumping activities. The results from these simulations serve as a partial analogue of future hydrologic conditions where ARs are expected to intensify and provide a greater fraction of annual precipitation due to climate change.
Photoelectron Boundary: The Top of the Dayside Ionosphere at Mars
Authors: Xu Shaosui , Mitchell David L. , McFadden James P. , Fowler Christopher M. , Hanley Kathleen , Weber Tristan , Brain David A. , Ma Yingjuan , DiBraccio Gina A. , Mazelle Christian , Curry Shannon M.
Published: May 2023

The interaction between Mars and the solar wind results in different plasma regimes separated by several boundaries, among which the separation between the sheath flow and the ionosphere is complicated. Previous studies have provided different and sometimes opposite findings regarding this region. In this study, we utilize observations from the Mars Atmospheric and Volatile EvolutioN (MAVEN) mission to revisit boundaries within this region and perhaps reconcile some differences. More specifically, we start with the photoelectron boundary (PEB), a topological boundary that separates magnetic field lines having access to the dayside ionosphere (open or closed) from those connected to the solar wind on both ends (draped). We find that large gradients in the planetary ion densiti occur across the PEB and that the dominant ion switches from heavy planetary ions to protons near the PEB, indicating that the PEB falls within the ion composition boundary (ICB). Furthermore, our results show that the PEB is not a pressure balance boundary; rather the magnetic pressure dominates both sides of the PEB. Meanwhile, we find that the PEB is located where the shocked solar wind flow stops penetrating deeper into the ionosphere. These findings suggest the PEB marks the top of the Mars dayside ionosphere and also the interface where the sheath plasma flow deflects around the obstacle going downstream.
Cell-cycle dependent nuclear gene delivery enhances the effects of E-cadherin against tumor invasion and metastasis
Authors: Xie Liting , Wang Jieqiong , Song Liming , Jiang Tianan , Yan Fei
Published: May 2023

Gene delivery is the process by which foreign DNA is transferred to host cells, released from intracellular vesicles, and transported to the nuclei for transcription. This process is frequently inefficient and difficult to control spatiotemporally. We developed a gene delivery strategy that uses ultrasound to directly deliver plasmid DNA into nuclei via gas vesicles (GVs)-based intracellular cavitation. pDNA-binding GVs can be taken up by cells and cause intracellular cavitation when exposed to acoustic irradiation and delivering their pDNA payloads into nuclei. Importantly, GVs can remain stable in the cytoplasm in the absence of acoustic irradiation, allowing for temporally controlled nuclear gene delivery. We were able to achieve spatiotemporal control of E-cadherin nuclear gene delivery in this manner, demonstrating its efficacy in tumor invasion and metastasis inhibition. Interestingly, we discovered that nuclear gene delivery of E-cadherin during the G2/M phase of the cell cycle in C6 tumor cells inhibited tumor invasion and metastasis more effectively than during the G1 and S phases. The gene delivery of E-cadherin at the G2/M phase resulted in significantly lower expression of Fam50a, which reduced Fam50a/Runx2 interaction and led to reduced transactivation of MMP13, an important factor for epithelial-mesenchymal transition, as observed in a molecular mechanism assay. Thus, using remote acoustic control of intracellular cavitation of pDNA-GVs, we developed a high spatiotemporally controllable gene delivery strategy and achieved stronger tumor invasion and metastasis inhibition effects by delivering the E-cadherin gene at the G2/M phase.
KLF12 promotes the proliferation of breast cancer cells by reducing the transcription of p21 in a p53-dependent and p53-independent manner
Authors: Li Yanan , Li Shujing , Shi Xiaoxia , Xin Zhiqiang , Yang Yuxi , Zhao Binggong , Li Yvlin , Lv Linlin , Ren Ping , Wu Huijian
Published: May 2023

Breast cancer is the most common cancer affecting women worldwide. Many genes are involved in the development of breast cancer, including the Kruppel Like Factor 12 (KLF12) gene, which has been implicated in the development and progression of several cancers. However, the comprehensive regulatory network of KLF12 in breast cancer has not yet been fully elucidated. This study examined the role of KLF12 in breast cancer and its associated molecular mechanisms. KLF12 was found to promote the proliferation of breast cancer and inhibit apoptosis in response to genotoxic stress. Subsequent mechanistic studies showed that KLF12 inhibits the activity of the p53/p21 axis, specifically by interacting with p53 and affecting its protein stability via influencing the acetylation and ubiquitination of lysine370/372/373 at the C-terminus of p53. Furthermore, KLF12 disrupted the interaction between p53 and p300, thereby reducing the acetylation of p53 and stability. Meanwhile, KLF12 also inhibited the transcription of p21 independently of p53. These results suggest that KLF12 might have an important role in breast cancer and serve as a potential prognostic marker and therapeutic target.
Venlafaxine antagonizes the noradrenaline-promoted colon cancer progression by inhibiting the norepinephrine transporter
Authors: Zhang Huahua , Han Jiming , Zhang Jing , Miao Jiyu , Li Fang , Tang Kaijie , Zhou Kai , Duan Baojun , Li Wen , Cheng Jing , Sun Ying , Hou Ni , Huang Chen
Published: May 2023

Epidemiological studies have demonstrated that the use of antidepressants is associated with a decreased risk of colorectal cancer (CRC); however, the mechanisms behind this association are yet unknown. Adrenergic system contributes to the stress-related tumor progression, with norepinephrine (NE) mainly secreted from adrenergic nerve fibers. Norepinephrine serotonin reuptake inhibitors are successfully used antidepressants. This study demonstrates that a widely used antidepressant venlafaxine (VEN) antagonizes NE-promoted colon cancer in vivo and in vitro. Bioinformatic analysis suggested that NE transporter (NET, SLC6A2), a target of VEN, was closely associated with the prognosis of clinical patients with CRC. In addition, the knockdown of NET antagonized the effect of NE. The NET-protein phosphatase 2 scaffold subunit alpha/phosphorylated Akt/vascular endothelial growth factor pathway partially mediates the antagonizing effect of VEN on NE’s actions in colon cancer cells. These were also confirmed by in vivo experiments. Our findings revealed for the first time that, in addition to its primary function as a transporter, NET also promotes NE-enhanced colon cancer cell proliferation, tumor angiogenesis, and tumor growth. This provides direct experimental and mechanistic evidence for the use of antidepressant VEN in the treatment of CRC and a therapeutic potential for repurposing existing drugs as an anti-cancer approach to improve the prognosis of patients with CRC.
RNA N6-methyladenosine reader IGF2BP3 interacts with MYCN and facilitates neuroblastoma cell proliferation
Authors: Zhu Kai , Gao Tingting , Wang Zhiru , Zhang Liaoran , Tan Kezhe , Lv Zhibao
Published: May 2023

Neuroblastoma (NB) is a kind of typical life-threatening extracranial tumor in children. N6-methyladenosine (m6A) modification is closely related to multiple cancer pathological processes. Insulin-like growth factor 2 mRNA binding protein 3 (IGF2BP3) is a top-ranked prognostic risk gene in NB; however, its function is uncertain. The expression of m6A-associated enzymes in patients with NB was analyzed using the Gene Expression Omnibus (GEO) and Therapeutically Applicable Research to Generate Effective Treatments (TARGET) database. The IGF2BP3 level in NB cell lines and primary samples was tested using quantitative real-time polymerase chain reaction (qRT-PCR), western blot method, and immunohistochemical analysis. The IGF2BP3 function in cell proliferation was clarified based on many functional in vitro and in vivo experiments. The interaction between IGF2BP3 and N-myc was researched via RNA immunoprecipitation (RIP), m6A RNA immunoprecipitation (MeRIP), and chromatin immunoprecipitation (ChIP) assays. The 16 m6A-regulated enzymes in NB were researched, and the result indicated that IGF2BP3 overexpression was related to cancer progression, COG risk, and survival based on the GEO and TARGET databases. Besides, the IGF2BP3 and MYCN levels were positively correlated. IGF2BP3 expression levels increased in MYCN-amplified NB clinical samples and cells. Knockdown of IGF2BP3 inhibited N-myc expression and NB cell proliferation in vitro and in vivo. IGF2BP3 regulates MYCN RNA stability by modifying m6A. In addition, we demonstrated that N-myc is a transcription factor that directly promotes IGF2BP3 expression in NB cells. IGF2BP3 regulates the proliferation of NB cells via m6A modification of MYCN. N-myc also acts as a transcription factor that regulates IGF2BP3 expression. A positive feedback loop between IGF2BP3 and N-myc facilitates NB cell proliferation.
Optical coherence tomography-based misdiagnosis and morphological distinction in pachychoroid neovasculopathy vs. polypoidal choroidal vasculopathy
Authors: Siedlecki Jakob , Klaas Julian , Keidel Leonie , Asani Ben , Schiefelbein Johannes , Knebel Dominik , Luft Nikolaus , Priglinger Siegfried G. , Schworm Benedikt
Published: May 2023

Purpose

To evaluate the rate of misdiagnosis of aneurysmatic pachychoroid type 1 choroidal neovascularization/polypoidal choroidal vasculopathy (PAT1/PCV) among cases diagnosed as non-aneurysmatic pachychoroid neovasculopathy (PNV) and to define optical coherence tomography (OCT) features facilitating their distinction.

Methods

The database of the Department of Ophthalmology, Ludwig-Maximilians University Munich, was screened for patients diagnosed with PNV. Multimodal imaging was screened for the presence of choroidal neovascularization (CNV) and aneurysms/polyps. Imaging features facilitating the diagnosis of PAT1/PCV were analysed.

Results

In total, 49 eyes of 44 patients with a clinical PNV diagnosis were included, of which 42 (85.7%) had PNV and 7 (14.3%) represented misdiagnosed PAT1/PCV. SFCT was comparable (PNV: 377 ± 92 vs. PAT1/PCV: 400 ± 83 µm; p = 0.39). Whereas no difference was detected in total pigment epithelium detachment (PED) diameter (p = 0.46), maximum PED height was significantly higher in the PAT1/PCV group (199 ± 31 vs. 82 ± 46, p < 0.00001). In a receiver operating characteristic (ROC) analysis, the optimum cutoff for defining “peaking PED” was 158 µm with an area under the curve of 0.969, a sensitivity of 1.0 (95% CI: 0.59–1.0), and a specificity of 0.95 (95% CI: 0.84–0.99). Sub-retinal hyperreflective material (SHRM; p = 0.04), sub-retinal ring-like structures (SRRLS; p < 0.00001), and sub-RPE fluid (p = 0.04) were significantly more frequent in eyes with PAT1/PCV.

Conclusion

A relevant percentage of eyes diagnosed with PNV might instead suffer from PAT1/PCV. The detection of a maximum PED height (“peaking PED”) exceeding approximately 150 µm, SHRM, SRRLS, and sub-RPE fluid might greatly aid in the production of a more accurate diagnosis.
Hierarchical triphase diffusion photoelectrodes for photoelectrochemical gas/liquid flow conversion
Authors: Meng Xiangyu , Zhu Chuntong , Wang Xin , Liu Zehua , Zhu Mengmeng , Yin Kuibo , Long Ran , Gu Liuning , Shao Xinxing , Sun Litao , Sun Yueming , Dai Yunqian , Xiong Yujie
Published: May 2023

Photoelectrochemical device is a versatile platform for achieving various chemical transformations with solar energy. However, a grand challenge, originating from mass and electron transfer of triphase—reagents/products in gas phase, water/electrolyte/products in liquid phase and catalyst/photoelectrode in solid phase, largely limits its practical application. Here, we report the simulation-guided development of hierarchical triphase diffusion photoelectrodes, to improve mass transfer and ensure electron transfer for photoelectrochemical gas/liquid flow conversion. Semiconductor nanocrystals are controllably integrated within electrospun nanofiber-derived mat, overcoming inherent brittleness of semiconductors. The mechanically strong skeleton of free-standing mat, together with satisfactory photon absorption, electrical conductivity and hierarchical pores, enables the design of triphase diffusion photoelectrodes. Such a design allows photoelectrochemical gas/liquid conversion to be performed continuously in a flow cell. As a proof of concept, 16.6- and 4.0-fold enhancements are achieved for the production rate and product selectivity of methane conversion, respectively, with remarkable durability.
Genetics implicates overactive osteogenesis in the development of diffuse idiopathic skeletal hyperostosis
Authors: Sethi Anurag , Ruby J. Graham , Veras Matthew A. , Telis Natalie , Melamud Eugene
Published: May 2023

Diffuse idiopathic skeletal hyperostosis (DISH) is a condition where adjacent vertebrae become fused through formation of osteophytes. The genetic and epidemiological etiology of this condition is not well understood. Here, we implemented a machine learning algorithm to assess the prevalence and severity of the pathology in ~40,000 lateral DXA scans in the UK Biobank Imaging cohort. We find that DISH is highly prevalent, above the age of 45, ~20% of men and ~8% of women having multiple osteophytes. Surprisingly, we find strong phenotypic and genetic association of DISH with increased bone mineral density and content throughout the entire skeletal system. Genetic association analysis identified ten loci associated with DISH, including multiple genes involved in bone remodeling (RUNX2, IL11, GDF5, CCDC91, NOG, and ROR2). Overall, this study describes genetics of DISH and implicates the role of overactive osteogenesis as a key driver of the pathology.
Structural mechanisms of TRPM7 activation and inhibition
Authors: Nadezhdin Kirill D. , Correia Leonor , Narangoda Chamali , Patel Dhilon S. , Neuberger Arthur , Gudermann Thomas , Kurnikova Maria G. , Chubanov Vladimir , Sobolevsky Alexander I.
Published: May 2023

The transient receptor potential channel TRPM7 is a master regulator of the organismal balance of divalent cations that plays an essential role in embryonic development, immune responses, cell mobility, proliferation, and differentiation. TRPM7 is implicated in neuronal and cardiovascular disorders, tumor progression and has emerged as a new drug target. Here we use cryo-EM, functional analysis, and molecular dynamics simulations to uncover two distinct structural mechanisms of TRPM7 activation by a gain-of-function mutation and by the agonist naltriben, which show different conformational dynamics and domain involvement. We identify a binding site for highly potent and selective inhibitors and show that they act by stabilizing the TRPM7 closed state. The discovered structural mechanisms provide foundations for understanding the molecular basis of TRPM7 channelopathies and drug development.
4D nanoimaging of early age cement hydration
Authors: Shirani Shiva , Cuesta Ana , Morales-Cantero Alejandro , Santacruz Isabel , Diaz Ana , Trtik Pavel , Holler Mirko , Rack Alexander , Lukic Bratislav , Brun Emmanuel , Salcedo Inés R. , Aranda Miguel A. G.
Published: May 2023

Despite a century of research, our understanding of cement dissolution and precipitation processes at early ages is very limited. This is due to the lack of methods that can image these processes with enough spatial resolution, contrast and field of view. Here, we adapt near-field ptychographic nanotomography to in situ visualise the hydration of commercial Portland cement in a record-thick capillary. At 19 h, porous C-S-H gel shell, thickness of 500 nm, covers every alite grain enclosing a water gap. The spatial dissolution rate of small alite grains in the acceleration period, ∼100 nm/h, is approximately four times faster than that of large alite grains in the deceleration stage, ∼25 nm/h. Etch-pit development has also been mapped out. This work is complemented by laboratory and synchrotron microtomographies, allowing to measure the particle size distributions with time. 4D nanoimaging will allow mechanistically study dissolution-precipitation processes including the roles of accelerators and superplasticizers.
Structure of the actively translating plant 80S ribosome at 2.2 Å resolution
Authors: Smirnova Julia , Loerke Justus , Kleinau Gunnar , Schmidt Andrea , Bürger Jörg , Meyer Etienne H. , Mielke Thorsten , Scheerer Patrick , Bock Ralph , Spahn Christian M. T. , Zoschke Reimo
Published: May 2023

In plant cells, translation occurs in three compartments: the cytosol, the plastids and the mitochondria. While the structures of the (prokaryotic-type) ribosomes in plastids and mitochondria are well characterized, high-resolution structures of the eukaryotic 80S ribosomes in the cytosol have been lacking. Here the structure of translating tobacco (Nicotiana tabacum) 80S ribosomes was solved by cryo-electron microscopy with a global resolution of 2.2 Å. The ribosome structure includes two tRNAs, decoded mRNA and the nascent peptide chain, thus providing insights into the molecular underpinnings of the cytosolic translation process in plants. The map displays conserved and plant-specific rRNA modifications and the positions of numerous ionic cofactors, and it uncovers the role of monovalent ions in the decoding centre. The model of the plant 80S ribosome enables broad phylogenetic comparisons that reveal commonalities and differences in the ribosomes of plants and those of other eukaryotes, thus putting our knowledge about eukaryotic translation on a firmer footing.
Time to workforce exit after a Parkinson’s disease diagnosis
Authors: Timpka Jonathan , Dahlström Örjan , Nilsson Maria H. , Iwarsson Susanne , Odin Per
Published: May 2023

The impact of Parkinson’s disease (PD) on workforce participation has received little attention even though demographic, lifestyle, and political changes together will result in an increased burden of PD on the working-age population. In this study, we investigate workforce survival after a PD diagnosis, as well as what demographic factors that are associated with workforce survival. As an exploratory outcome, we investigate workforce survival in persons with and without device-aided treatment (DAT). This is a nested case-cohort study based on Swedish national data from 2001–2016. Controls were matched on year of birth, sex, and municipality of residence. The used registers contain data on demographics, social insurance, in- and outpatient visits, filled drug prescriptions, and cause of death on the person-level. A total of 4781 persons with PD and 23,905 controls were included. The median survival until all-cause workforce exit was 43 months among persons that were workforce-active at the time of PD diagnosis, compared to 66 months in non-PD controls. Being female, ≥50 years old at diagnosis, or having a lower education were contributing factors to health-related workforce exit. Persons receiving DAT during follow-up exhibited shorter workforce survival than controls. However, this needs further investigation, particularly as patients have generally already left the workforce at the time for start of DAT. It is evident that PD has grave negative effects on workforce participation. Thus, supportive measures need to start at an early stage after diagnosis, and the development of new interventions is urgently needed.
Origin of the exotic electronic states in antiferromagnetic NdSb
Authors: Li Peng , Li Tongrui , Liao Sen , Cao Zhipeng , Xu Rui , Wang Yuzhe , Yao Jianghao , Cui Shengtao , Sun Zhe , Wang Yilin , Wan Xiangang , Jiang Juan , Feng Donglai
Published: May 2023

Using angle resolved photoemission spectroscopy measurements and first principle calculations, we report that the possible unconventional 2q antiferromagnetic (AFM) order in NdSb can induce unusual modulation on its electronic structure. The obvious extra bands observed in the AFM phase of NdSb are well reproduced by theoretical calculations, in which the Fermi-arc-like structures and sharp extra bands are originated from the in-gap surface states. However, they are demonstrated to be topological trivial. By tuning the chemical potential, the AFM phase of NdSb would go through a topological phase transition, realizing a magnetic topological insulator phase. Hence, our study sheds new light on the rare earth monopnictides for searching unusual AFM structure and the potential of intrinsic magnetic topological materials.
Antiferromagnetic half-skyrmions electrically generated and controlled at room temperature
Authors: Amin O. J. , Poole S. F. , Reimers S. , Barton L. X. , Dal Din A. , Maccherozzi F. , Dhesi S. S. , Novák V. , Krizek F. , Chauhan J. S. , Campion R. P. , Rushforth A. W. , Jungwirth T. , Tretiakov O. A. , Edmonds K. W. , Wadley P.
Published: May 2023

Topologically protected magnetic textures are promising candidates for information carriers in future memory devices, as they can be efficiently propelled at very high velocities using current-induced spin torques. These textures—nanoscale whirls in the magnetic order—include skyrmions, half-skyrmions (merons) and their antiparticles. Antiferromagnets have been shown to host versions of these textures that have high potential for terahertz dynamics, deflection-free motion and improved size scaling due to the absence of stray field. Here we show that topological spin textures, merons and antimerons, can be generated at room temperature and reversibly moved using electrical pulses in thin-film CuMnAs, a semimetallic antiferromagnet that is a testbed system for spintronic applications. The merons and antimerons are localized on 180° domain walls, and move in the direction of the current pulses. The electrical generation and manipulation of antiferromagnetic merons is a crucial step towards realizing the full potential of antiferromagnetic thin films as active components in high-density, high-speed magnetic memory devices.
Stable perovskite single-crystal X-ray imaging detectors with single-photon sensitivity
Authors: Sakhatskyi Kostiantyn , Turedi Bekir , Matt Gebhard J. , Wu Erfu , Sakhatska Anastasiia , Bartosh Vitalii , Lintangpradipto Muhammad Naufal , Naphade Rounak , Shorubalko Ivan , Mohammed Omar F. , Yakunin Sergii , Bakr Osman M. , Kovalenko Maksym V.
Published: May 2023

A major thrust of medical X-ray imaging is to minimize the X-ray dose acquired by the patient, down to single-photon sensitivity. Such characteristics have been demonstrated with only a few direct-detection semiconductor materials such as CdTe and Si; nonetheless, their industrial deployment in medical diagnostics is still impeded by elaborate and costly fabrication processes. Hybrid lead halide perovskites can be a viable alternative owing to their facile solution growth. However, hybrid perovskites are unstable under high-field biasing in X-ray detectors, owing to structural lability and mixed electronic–ionic conductivity. Here we show that both single-photon-counting and long-term stable performance of perovskite X-ray detectors are attained in the photovoltaic mode of operation at zero-voltage bias, employing thick and uniform methylammonium lead iodide single-crystal films (up to 300 µm) and solution directly grown on hole-transporting electrodes. The operational device stability exceeded one year. Detection efficiency of 88% and noise-equivalent dose of 90 pGyair are obtained with 18 keV X-rays, allowing single-photon-sensitive, low-dose and energy-resolved X-ray imaging. Array detectors demonstrate high spatial resolution up to 11 lp mm−1. These findings pave the path for the implementation of hybrid perovskites in low-cost, low-dose commercial detector arrays for X-ray imaging.
Structural basis of metabolite transport by the chloroplast outer envelope channel OEP21
Authors: Günsel Umut , Klöpfer Kai , Häusler Elisabeth , Hitzenberger Manuel , Bölter Bettina , Sperl Laura E. , Zacharias Martin , Soll Jürgen , Hagn Franz
Published: May 2023

Triose phosphates (TPs) are the primary products of photosynthetic CO2fixation in chloroplasts, which need to be exported into the cytosol across the chloroplast inner envelope (IE) and outer envelope (OE) membranes to sustain plant growth. While transport across the IE is well understood, the mode of action of the transporters in the OE remains unclear. Here we present the high-resolution nuclear magnetic resonance (NMR) structure of the outer envelope protein 21 (OEP21) from garden pea, the main exit pore for TPs in C3plants. OEP21 is a cone-shaped β-barrel pore with a highly positively charged interior that enables binding and translocation of negatively charged metabolites in a competitive manner, up to a size of ~1 kDa. ATP stabilizes the channel and keeps it in an open state. Despite the broad substrate selectivity of OEP21, these results suggest that control of metabolite transport across the OE might be possible.
Coastal survey data for Perranporth Beach and Start Bay in southwest England (2006–2021)
Authors: McCarroll R. J. , Valiente N. G. , Wiggins M. , Scott T. , Masselink G.
Published: May 2023

Records of beach morphologic change and concurrent hydrodynamic forcing are needed to understand how coastlines in different environments change over time. This submission contains data for the period 2006 to 2021, for two contrasting macrotidal environments in southwest England: (i) cross-shore dominated, dissipative, sandy Perranporth Beach, Cornwall; and (ii) longshore-dominated, reflective gravel beaches within Start Bay, Devon. Data comprise monthly to annual beach profile surveys, annual merged topo-bathymetries, in addition to observed and numerically modelled wave and water levels. These data provide a valuable resource for modelling the behaviour of coastal types not covered by other currently available datasets.
Unique inverse association between allergic rhinitis and periodontitis: a nationwide population-based study
Authors: Kim Dae-Yeob , Lee Jae-Kwan , Pang Eun-Kyoung , Choi Seong-Ho , Lee Jong-Bin
Published: May 2023

The increase in fine dust levels in the atmosphere has been associated with a growth in the incidence of environmental diseases, including allergic rhinitis (AR). Nasal obstruction caused by AR can impact the conditions in the oral cavity. The aim of this study was to determine the association between AR and periodontitis in the Republic of Korea. This study was based on data from the Seventh Korea National Health and Nutrition Examination Survey (KNHANES VII-1, 2016), which was conducted by the Korea Centers for Disease Control and Prevention. The study included 6129 adults older than 19 years. Sociodemographic information and medical variables including history of treatment of periodontitis (HTP) reflecting diagnosis of periodontitis and diagnosis of diseases such as AR were extracted from the data. HTP and AR were reported for 22.81 ± 0.84% (weighted percentage ± standard error) and 15.32 ± 0.63% of the studied population, respectively. A diagnosis of AR was reported for 11.07 ± 1.28% of those with HTP and for 17.55 ± 1.84% of those without HTP. From these, it was inferred that the prevalence of HTP was 1.536-fold higher in the non-AR group than in their counterparts with AR. Significant association was found between AR and HTP among those aged ≤ 64 years and the odds ratio (OR) of AR group for HTP was 0.62 (95% confidence interval:0.44–0.87; P = 0.0057). From this result, it can be inferred that patients diagnosed AR have lower risk of periodontitis.
Assessing the application of the revised Remane Model to fish species in a fluvially dominated cool-temperate southern African coastal system
Authors: Nashima Festus P. , Strydom Nadine A. , Lamberth Stephen J.
Published: May 2023

Estuaries are ecologically important areas which support a variety of aquatic species, particularly marine and estuarine fish species. This study represents a case study of the Orange River and Estuary (ORE) to understand patterns in fish assemblages and diversity trends that were compared to the revised Remane Model predictions in an estuary with poor marine fish species richness. A total of 30 species were recorded in the River continuum that comprised 14 freshwater, 10 marine and six estuarine species. Species diversity revealed seasonal variation in fish assemblages during the high-flow and low-flow seasons but not inter-annually. The results revealed that species diversity is lower in higher salinity areas when compared to low salinity areas. These patterns are consistent with the biogeographical trend of decreasing species richness along the South African coast from east to west, however, inconsistent with Remane predictions. The extremely low marine fish richness at its lower end and the extremely large freshwater influx at its upper end are the primary causes of the inconsistency. This may reflects the unsuitability of the Remane model for the Orange Estuary. In comparison to similar river-dominated South African estuaries, the ORE has a low marine species richness. When compared to more conventional South African estuaries, the ORE has a unique biotic environment with low fish species richness of estuarine-associated marine species adjacent to the Benguela upwelling zone and therefore the region does not offer suitable habitat for such species. As a result, the ORE is not a good candidate to test the Remane Model. The data does, however, confirm the left-hand part of the Remane model, which shows a decline in the fish species richness of freshwater fish species as salinity rises towards mesohaline and polyhaline levels.
The value of EYA1/3/4 in clear cell renal cell carcinoma: a study from multiple databases
Authors: Liu Taobin , Nie Jianqiang , Zhang Xiaoming , Deng Xinxi , Fu Bin
Published: May 2023

There is evidence from multiple studies that dysregulation of the Eyes Absent (EYA) protein plays multiple roles in many cancers. Despite this, little is known about the prognostic significance of the EYAs family in clear cell renal cell carcinoma (ccRCC). We systematically analyzed the value of EYAs in Clear Cell Renal Cell Carcinoma. Our analysis included examining transcriptional levels, mutations, methylated modifications, co-expression, protein–protein interactions (PPIs), immune infiltration, single-cell sequencing, drug sensitivity, and prognostic values. We based our analysis on data from several databases, including the Cancer Genome Atlas database (TCGA), the Gene Expression Omnibus database (GEO), UALCAN, TIMER, Gene Expression Profiling Interactive Analysis (GEPIA), STRING, cBioPortal and GSCALite. In patients with ccRCC, the EYA1 gene was significantly highly expressed, while the expression of EYA2/3/4 genes showed the opposite trend. The level of expression of the EYA1/3/4 gene was significantly correlated with the prognosis and clinicopathological parameters of ccRCC patients. Univariate and multifactorial Cox regression analyses revealed EYA1/3 as an independent prognostic factor for ccRCC, establishing nomogram line plots with good predictive power. Meanwhile, the number of mutations in EYAs was also significantly correlated with poor overall survival (OS) and progression-free survival (PFS) of patients with ccRCC. Mechanistically, EYAs genes play an essential role in a wide range of biological processes such as DNA metabolism and double-strand break repair in ccRCC. The majority of EYAs members were related to the infiltration of immune cells, drug sensitivity, and methylation levels. Furthermore, our experiment confirmed that EYA1 gene expression was upregulated, and EYA2/3/4 showed low expression in ccRCC. The increased expression of EYA1 might play an important role in ccRCC oncogenesis, and the decreased expression of EYA3/4 could function as a tumor suppressor, suggesting EYA1/3/4 might serve as valuable prognostic markers and potential new therapeutic targets for ccRCC.
CoVnita, an end-to-end privacy-preserving framework for SARS-CoV-2 classification
Authors: Sim Jun Jie , Zhou Weizhuang , Chan Fook Mun , Annamalai Meenatchi Sundaram Muthu Selva , Deng Xiaoxia , Tan Benjamin Hong Meng , Aung Khin Mi Mi
Published: May 2023

Classification of viral strains is essential in monitoring and managing the COVID-19 pandemic, but patient privacy and data security concerns often limit the extent of the open sharing of full viral genome sequencing data. We propose a framework called CoVnita, that supports private training of a classification model and secure inference with the same model. Using genomic sequences from eight common SARS-CoV-2 strains, we simulated scenarios where the data was distributed across multiple data providers. Our framework produces a private federated model, over 8 parties, with a classification AUROC of 0.99, given a privacy budget of $$\varepsilon =1$$

ε
=
1

. The roundtrip time, from encryption to decryption, took a total of 0.298 s, with an amortized time of 74.5 ms per sample.
Deep fake detection and classification using error-level analysis and deep learning
Authors: Rafique Rimsha , Gantassi Rahma , Amin Rashid , Frnda Jaroslav , Mustapha Aida , Alshehri Asma Hassan
Published: May 2023

Due to the wide availability of easy-to-access content on social media, along with the advanced tools and inexpensive computing infrastructure, has made it very easy for people to produce deep fakes that can cause to spread disinformation and hoaxes. This rapid advancement can cause panic and chaos as anyone can easily create propaganda using these technologies. Hence, a robust system to differentiate between real and fake content has become crucial in this age of social media. This paper proposes an automated method to classify deep fake images by employing Deep Learning and Machine Learning based methodologies. Traditional Machine Learning (ML) based systems employing handcrafted feature extraction fail to capture more complex patterns that are poorly understood or easily represented using simple features. These systems cannot generalize well to unseen data. Moreover, these systems are sensitive to noise or variations in the data, which can reduce their performance. Hence, these problems can limit their usefulness in real-world applications where the data constantly evolves. The proposed framework initially performs an Error Level Analysis of the image to determine if the image has been modified. This image is then supplied to Convolutional Neural Networks for deep feature extraction. The resultant feature vectors are then classified via Support Vector Machines and K-Nearest Neighbors by performing hyper-parameter optimization. The proposed method achieved the highest accuracy of 89.5% via Residual Network and K-Nearest Neighbor. The results prove the efficiency and robustness of the proposed technique; hence, it can be used to detect deep fake images and reduce the potential threat of slander and propaganda.
Melatonin influences methyl jasmonate-induced protection of photosynthetic activity in wheat plants against heat stress by regulating ethylene-synthesis genes and antioxidant metabolism
Authors: Sehar Zebus , Fatma Mehar , Khan Sheen , Mir Iqbal R. , Abdi Gholamreza , Khan Nafees A.
Published: May 2023

Melatonin (MT) and methyl jasmonate (MeJA) play important roles in the adaptation of plants to different stress factors by modulating stress tolerance mechanisms. The present study reports the involvement of MT (100 µM) in MeJA (10 µM)-induced photosynthetic performance and heat stress acclimation through regulation of the antioxidant metabolism and ethylene production in wheat (Triticum aestivum L.) plants. Plants exposed to 40 °C for 6 h per day for 15 days and allowed to retrieve at 28 °C showed enhanced oxidative stress and antioxidant metabolism, increased 1-aminocyclopropane-1-carboxylic acid (ACC) synthase (ACS) activity and ethylene production, and decreased photosynthetic performance. In contrast, the exogenously applied MT and MeJA reduced oxidative stress through improved S-assimilation (+ 73.6% S content), antioxidant defense system (+ 70.9% SOD, + 115.8% APX and + 104.2% GR, and + 49.5% GSH), optimized ethylene level to 58.4% resulting in improved photosynthesis by 75%. The use of p-chlorophenyl alanine, a MT biosynthesis inhibitor along with MeJA in the presence of heat stress reduced the photosynthetic performance, ATP-S activity and GSH content, substantiated the requirement of MT in the MeJA-induced photosynthetic response of plants under heat stress. These findings suggest that MeJA evoked the plant’s ability to withstand heat stress by regulating the S-assimilation, antioxidant defense system, and ethylene production, and improving photosynthetic performance was dependent on MT.
Short hydrophobic loop motifs in BRICHOS domains determine chaperone activity against amorphous protein aggregation but not against amyloid formation
Authors: Chen Gefei , Leppert Axel , Poska Helen , Nilsson Harriet E. , Alvira Carlos Piedrafita , Zhong Xueying , Koeck Philip , Jegerschöld Caroline , Abelein Axel , Hebert Hans , Johansson Jan
Published: May 2023

ATP-independent molecular chaperones are important for maintaining cellular fitness but the molecular determinants for preventing aggregation of partly unfolded protein substrates remain unclear, particularly regarding assembly state and basis for substrate recognition. The BRICHOS domain can perform small heat shock (sHSP)-like chaperone functions to widely different degrees depending on its assembly state and sequence. Here, we observed three hydrophobic sequence motifs in chaperone-active domains, and found that they get surface-exposed when the BRICHOS domain assembles into larger oligomers. Studies of loop-swap variants and site-specific mutants further revealed that the biological hydrophobicities of the three short motifs linearly correlate with the efficiency to prevent amorphous protein aggregation. At the same time, they do not at all correlate with the ability to prevent ordered amyloid fibril formation. The linear correlations also accurately predict activities of chimeras containing short hydrophobic sequence motifs from a sHSP that is unrelated to BRICHOS. Our data indicate that short, exposed hydrophobic motifs brought together by oligomerisation are sufficient and necessary for efficient chaperone activity against amorphous protein aggregation.
Mercury isotope evidence for marine photic zone euxinia across the end-Permian mass extinction
Authors: Sun Ruoyu , Liu Yi , Sonke Jeroen E. , Feifei Zhang , Zhao Yaqiu , Zhang Yonggen , Chen Jiubin , Liu Cong-Qiang , Shen Shuzhong , Anbar Ariel D. , Zheng Wang
Published: May 2023

The driving forces, kill and recovery mechanisms for the end-Permian mass extinction (EPME), the largest Phanerozoic biological crisis, are under debate. Sedimentary records of mercury enrichment and mercury isotopes have suggested the impact of volcanism on the EPME, yet the causes of mercury enrichment and isotope variations remain controversial. Here, we model mercury isotope variations across the EPME to quantitatively assess the effects of volcanism, terrestrial erosion and photic zone euxinia (PZE, toxic, sulfide-rich conditions). Our numerical model shows that while large-scale volcanism remains the main driver of widespread mercury enrichment, the negative shifts of Δ199Hg isotope signature across the EPME cannot be fully explained by volcanism or terrestrial erosion as proposed before, but require additional fractionation by marine mercury photoreduction under enhanced PZE conditions. Thus our model provides further evidence for widespread and prolonged PZE as a key kill mechanism for both the EPME and the impeded recovery afterward.
Impact of regiochemistry in energetic materials science: a case of (nitratomethyl-1,2,3-triazolyl)furazans
Authors: Epishina Margarita A. , Kulikov Alexander S. , Ananyev Ivan V. , Anisimov Aleksei A. , Monogarov Konstantin A. , Fershtat Leonid L.
Published: May 2023

Regiodivergent assembly of novel high-performance energetic melt-castable substances or plasticizers incorporating a (1,2,3-triazolyl)furazan scaffold was performed.
Surface crystallinity enhancement in organic solar cells induced by spinodal demixing of acceptors and additives
Authors: Shen Zichao , Yu Jinde , Lu Guanyu , Wu Keming , Wang Qingyu , Bu Laju , Liu Xinfeng , Zhu Yuanwei , Lu Guanghao
Published: May 2023

Surface spinodal demixing of DIO:ITIC leads to poor electron mobility, while the CN:Y6 blend undergoes a binodal demixing process which is similar to that occurs in the bulk liquid layer, contributing to uniform vertical crystallinity distribution.
Remarkable 980 nm circularly polarized luminescence from dinuclear Yb(iii) helicates with aD4symmetry
Authors: Wang Lingli , Yao Zhiwei , Huang Wenru , Gao Ting , Yan Pengfei , Zhou Yanyan , Li Hongfeng
Published: May 2023

Chiral BINOL-modified dinuclear helicates Yb2(R/S-BTHP)4show the excellent NIR-CPL at 980 nm with theglumreaching up to 0.81 andBCPLbeing up to 821 M−1cm−1.
Word Learning With Orthographic Support in Nonspeaking and Minimally Speaking School-Age Autistic Children
Authors: Clark Grace T. , Reuterskiöld Christina
Published: May 2023

Purpose:

Previous research has demonstrated that typically developing children, verbal children with a diagnosis of autism, children with Down syndrome, children with developmental language disorder, and children with dyslexia can all benefit from orthographic support during word learning tasks. This study sought to determine if minimally speaking or nonspeaking children with a diagnosis of autism would also demonstrate an orthographic facilitation effect during a computer-based remote word learning task.

Method:

Twenty-two school-age children with a diagnosis of autism and little to no spoken language learned four novel words by contrasting the words with known objects. Two novel words were taught with orthographic support present, and two were taught without orthographic support. Participants were exposed to the words a total of 12 times and then given an immediate posttest to assess identification. Parent report measures of receptive vocabulary, expressive vocabulary, autism symptomatology, and reading skills were also collected.

Results:

During learning tasks, participants performed equally well whether orthographic support was given or not. For the posttest, however, participants performed significantly better for words that were taught with orthographic support. The presence of orthography improved accuracy and supported a greater number of participants to reach the passing criterion compared to the absence of orthography. Orthographic representations aided the word learning of those with lower expressive language significantly more than those with higher expressive language.

Conclusions:

Minimally speaking or nonspeaking children with a diagnosis of autism benefit from orthographic support when learning new words. Further investigation is warranted to determine if this effect holds during face-to-face interactions using augmentative and alternative communication systems.

Supplemental Material:

https://doi.org/10.23641/asha.22465492
Design, fabrication, and realisation of a robotic fish actuated by dielectric elastomer with a passive fin
Authors: Wang Zekai , Lou Junqiang , Xiao Xingdong , Li Guoping , Deng Yimin
Published: May 2023

Robotic fish actuated by smart materials has attracted extensive attention and has been widely used in many applications. In this study, a robotic fish actuated by dielectric elastomer (DE) films is proposed. The tensile behaviours of DE film VHB4905 are studied, and the Ogden constitutive equation is employed to describe the stress‐strain behaviour of the DE film. The fabrication processes of the robotic fish, including pre‐stretching treatment of the DE films, electrode coating with carbon paste, and waterproof treatment, are illustrated in detail. The dynamic response of the fabricated DE actuators under different excitation voltages is tested based on the experimental setup. Experimental results show that the first‐order natural frequencies of the obtained DE actuator in air is 4.05 Hz. Finally, the swimming performances of the proposed robotic fish at different driving levels are demonstrated, and it achieves an average swimming speed of 20.38 mm/s, with a driving voltage of 5kV at 0.8 Hz.