bluuebunny/crossref_metadata_2025 huggingface dataset:
The prevalence of gestational diabetes mellitus before and after the implementation of the universal two-child policy in China
Authors: Zhu Hui , Zhao Zhijia , Xu Jin , Chen Yanming , Zhu Qiong , Zhou Liming , Cai Jie , Ji Lindan
Published: August 2022

Background

After the universal two-child policy has been fully implemented, challenges regarding pregnancy complications seemed to be more severe in China. This study aimed to evaluate the prevalence of gestational diabetes mellitus (GDM) and the main risk factors for GDM before and after the implementation of the universal two-child policy in China.

Methods

A retrospective study was performed with 128,270 pregnant women who delivered at Ningbo Women & Children’s Hospital from January 2010 to December 2020. Univariate and multivariate logistic regression analysis was applied to estimate the risk factors associated with GDM prevalence. Segmented regression analyses of interrupted time series (ITS) were conducted to assess the effect of the universal two-child policy on the trends of GDM.

Results

The prevalence of GDM increased remarkably from 4% in 2010 to 21% in 2020. ITS analysis presented that the prevalence of GDM increased by 0.190% (β1) per month from 2010 to 2016 (P<0.05), and by 0.044% (β1+β3) per month after the implementation of the universal two-child policy; the rate of elevation of GDM slowed down significantly (β3=-0.146, P=0.004). Advanced maternal age (>30 years), multigravidity, multiparity, multiple gestation and gestational hypertension were significantly associated with GDM. Advanced age remained an independent risk factor for GDM even after cross stratification with gravidity and parity. The proportion of women with advanced maternal age (>30 years) increased by 0.161% per month before the implementation of the universal two-child policy and increased by 5.25% during the policy took effect month, and gradually increased by 0.124% (β1+β3) per month after then.

Conclusions

The prevalence of GDM has sharply increased in the past decade. The growth rate of GDM slowed down after the implementation of the universal two-child policy in China, but the rate would maintain at a high plateau. The rise in the proportion of older pregnant women could increase the GDM rate. We recommend having children at a relatively optimal reproductive age when encouraging childbearing.
Research progress on the mechanism of beta-cell apoptosis in type 2 diabetes mellitus
Authors: You SuFang , Zheng JingYi , Chen YuPing , Huang HuiBin
Published: August 2022

Type 2 diabetes mellitus(T2DM) is regarded as one of the most severe chronic metabolic diseases worldwide, which poses a great threat to human safety and health. The main feature of T2DM is the deterioration of pancreatic beta-cell function. More and more studies have shown that the decline of pancreatic beta-cell function in T2DM can be attributable to beta-cell apoptosis, but the exact mechanisms of beta-cell apoptosis in T2DM are not yet fully clarified. Therefore, in this review, we will focus on the current status and progress of research on the mechanism of pancreatic beta-cell apoptosis in T2DM, to provide new ideas for T2DM treatment strategies.
C-peptide is a predictor of telomere shortening: A five-year longitudinal study
Authors: Ghoussaini Racha , Tamim Hani , Elbejjani Martine , Makki Maha , Nasreddine Lara , Ismaeel Hussain , Nasrallah Mona P. , Zgheib Nathalie K.
Published: August 2022

Aim

Relative telomere length (RTL) predicts the development of many age-related diseases. Yet, few studies have evaluated their longitudinal effect on RTL. We investigated longitudinally the association between cardiometabolic risk factors and RTL.

Methods

This was a longitudinal study with a 5-year follow-up period, based on data collected in 2014 and 2019. Of 478 participants in 2014, 198 consented to be followed-up in 2019. The associations between RTL and risk factors were analyzed using t-test, ANOVA or simple linear regression as applicable.

Results

RTL was significantly shortened after 5 years (P<0.001). Older age (P=0.018) and gender (P=0.05) were significantly associated with shorter RTL at follow-up. Higher baseline C-peptide correlated with shorter RTL (P=0.04) and shortening of RTL (P=0.03) after 5 years. Multivariate linear regression including both age and gender revealed a significant trend for C-peptide and change in RTL after 5 years (P=0.04). Interestingly, there was a trend of shorter RTL at follow-up with diabetes, though the findings were not statistically significant.

Conclusions

Higher C-peptide level contributes to telomere shortening over time, suggesting that metabolic dysregulation may play a role in early aging. Further understanding of this relationship and addressing high C-peptide levels can be important to prevent premature aging.
A new predictive model for the concurrent risk of diabetic retinopathy in type 2 diabetes patients and the effect of metformin on amino acids
Authors: Song Zicheng , Luo Weiming , Huang Bing , Cao Yunfeng , Jiang Rongzhen
Published: August 2022

Objective

This study established a model to predict the risk of diabetic retinopathy (DR) with amino acids selected by partial least squares (PLS) method, and evaluated the effect of metformin on the effect of amino acids on DR in the model.

Methods

In Jinzhou, Liaoning Province, China, we retrieved 1031 patients with type 2 diabetes (T2D) from the First Affiliated Hospital of Liaoning Medical University. After sorting the amino acids using the PLS method, the top 10 amino acids were included in the model. Multivariate logistic regression was used to analyze the relationship between different amino acids and DR. And then the effects of metformin on amino acids were explored through interaction. Finally, Spearman’s rank correlation analysis was used to analyze the correlation between different amino acids.

Results

After sorting by PLS, Gly, Pro, Leu, Lyr, Glu, Phe, Tyr, His, Val and Ser were finally included in the DR risk prediction model. The predictive model after adding amino acids was statistically different from the model that only included traditional risk factors (p=0.001). Metformin had a significant effect on the relationship between DR and 7 amino acids (Gly, Glu, Phe, Tyr, His, Val, Ser, p<0.05), and the population who are not using metformin and have high levels of Glu (OR: 0.44, 95%CI: 0.27-0.71) had an additive protection effect for the occurrence of DR. And the similar results can be seen in high levels of Gly (OR: 0.46, 95%CI: 0.29-0.75), Leu (OR: 0.48, 95%CI: 0.29-0.8), His (OR: 0.46, 95%CI: 0.29-0.75), Phe (OR: 0.24, 95%CI: 0.14-0.42) and Tyr (OR: 0.41, 95%CI: 0.24 -0.68) in population who are not using metformin.

Conclusions

We established a prediction model of DR by amino acids and found that the use of metformin reduced the protective effect of amino acids on DR developing, suggesting that amino acids as biomarkers for predicting DR would be affected by metformin use.
Impact of Natural Disaster Shocks on Farm Household Poverty Vulnerability—A Threshold Effect Based on Livelihood Resilience
Authors: Lu Honggang , Zheng Jiaxi , Ou Hanyun , Liu Yinong , Li Xijian
Published: August 2022

Poverty caused by disasters poses a great challenge to consolidate the achievements of poverty alleviation. Livelihood resilience is the key factor for farmers to resist risks and get rid of poverty. Therefore, this study used the China Family Panel Studies (CFPS) database. Firstly, we examined the impact of natural disasters on the poverty vulnerability of farmers. Secondly, taking livelihood resilience and its decomposition dimensions as threshold variables, we examined the mechanism of livelihood resilience between natural disasters and poverty. The results show that natural disaster shocks, natural disaster intensity, and natural disaster frequency all had a significant positive effect on farm households’ vulnerability to poverty. The threshold test shows that natural disasters had larger effects on the poverty vulnerability of the farmers with lower buffer capacity, self-organizing capacity, and learning capacity. When the livelihood resilience value exceeded the third threshold, the impact of natural disasters on the poverty vulnerability of farmers turned from positive to negative. When the buffer capacity exceeded the third threshold, the impact of natural disasters on poverty vulnerability turned from positive to negative; when the self-organizing capacity exceeded the first threshold, the impact of natural disasters on poverty vulnerability turned from positive to negative; when the learning capacity exceeded the third threshold, the impact of natural disasters on poverty vulnerability turned from positive to negative. Therefore, it is suggested that appropriate policies should be needed to support farmers’ livelihood resilience and address disaster-induced poverty by improving farmers’ buffer capacity, self-organizing capacity, and learning capacity. Focusing on farmers’ livelihood resilience, government should establish a policy support system aimed at improving farmers’ buffer capacity, self-organizing capacity, and learning capacity, that will help farmers to escape from disaster-induced poverty.
Ontogeny and individual heterogeneity of the corticosterone stress response in a wild altricial seabird, the snow petrel (Pagodroma nivea)
Authors: Angelier Frédéric , Wingfield John C. , Barbraud Christophe , Parenteau Charline , Trouvé Colette , Chastel Olivier
Published: August 2022

In the current context of global change, there is evidence of a large inter-individual variability in the way animals physiologically respond to anthropogenic changes. In that context, the Hypothalamus-Pituitary-Adrenal (HPA) axis and the corticosterone stress response are of primary importance because they are thought to govern the ability of individuals to adjust to stress. Several studies have reported that this stress response is variable among adults and they have successfully linked this variability with abiotic and biotic factors. However, the inter-individual variability of the glucocorticoid stress response has rarely been examined during the developmental phase in wild vertebrates, and its potential ecological determinants remain unclear. In this study, we examined the ontogeny of the corticosterone stress response in an altricial seabird species (i.e., how corticosterone levels increase in response to a standardized stress protocol), the Snow petrel. We reported a strong heterogeneity of the corticosterone stress response at all ages in snow petrel chicks (11-, 20-, and 37-days old chicks). Although the magnitude of this corticosterone stress response decreases with the age of the chick, we also found that this corticosterone stress response was repeatable throughout the post-hatching developmental period (repeatability: r > 0.50 for stress-induced corticosterone levels after a 30-min restraint). Importantly, this glucocorticoid stress response was negatively associated with the body condition of the chicks (i.e., mass corrected for body size), and previous exposure to sampling was associated with a dampened corticosterone stress response. However, we did not find any link between parental traits (parental condition or parental corticosterone stress response), nest quality, hatching date, and the chick’s corticosterone stress response. Our study suggests that the corticosterone stress response is a consistent individual trait that is affected to some extent by post-natal conditions, and which differs among individuals very early in life.
Tradeoffs and synergies among ecosystem services, biodiversity conservation, and food production in coffee agroforestry
Authors: Mayorga Isabella , Vargas de Mendonça Jose Luiz , Hajian-Forooshani Zachary , Lugo-Perez Javier , Perfecto Ivette
Published: August 2022

Concerns over the capacity of the world’s existing agricultural land to provide food for the global population under climate change and continued biodiversity loss have set the stage for a prevailing narrative of inherent tradeoffs with agricultural production. Coffee, a major export of tropical countries, offers a unique opportunity to examine how different management practices can lead to a variety of outcomes in food security, ecosystem services, and biodiversity conservation. Our study examined this intersection to identify tradeoffs and synergies using compiled data from Puerto Rico. At the island level, we analyzed data on coffee yield and planted area under shade or sun management. At the farm level, we analyzed management variables (percent shade cover, maximum canopy height, ground cover, and food crop richness), non-provisioning ecosystem services variables (total farm carbon storage, soil organic carbon storage, coffee plant carbon biomass, and hurricane resistance and resilience), and biodiversity variables (ant, bird, and lizard richness and abundance). At the island level, we found that planted area was the most significant predictor of total production, suggesting no obvious tradeoff between production and shade management in coffee farms. At the farm level, canopy cover of shade trees was negatively correlated with ground cover and positively correlated with food crop richness, suggesting a synergy between agroforestry and subsistence food production. We detected mostly synergies associated with ecosystem services, biodiversity conservation, and agroforestry management and no tradeoffs among ecosystem service and biodiversity parameters. Shade canopy cover significantly increased total carbon storage, coffee plant biomass, hurricane resistance, and bird species richness. Shade canopy height had a similar positive effect on total farm carbon storage while food crop richness had a positive effect on farm resilience following Hurricane Maria. Ground cover was positively associated with soil carbon storage and pest-controlling lizard abundance. Tradeoffs related to agroforestry management included an inverse relationship between ground cover and hurricane resistance and more dominance of an invasive ant species in farms with higher shade canopies. We discuss the implications of practicing agroforestry principles in this smallholder coffee system and highlight opportunities to contribute to more diversified food production systems that support biodiversity and ecosystem services.
Impacts of climate change on tropical agroforestry systems: A systematic review for identifying future research priorities
Authors: Watts Martin , Hutton Craig , Mata Guel Erik O. , Suckall Natalie , Peh Kelvin S.-H.
Published: August 2022

Climate change is expected to adversely affect the crop yields and food security for many smallholder farmers in the tropics unless adaptive measures are implemented. Agroforestry ecosystem services, such as micro-climate buffering, have received growing attention from the academic and policy communities for alleviating the negative impacts of climate change on smallholders. These benefits imply that agroforestry could offer a suitable measure for adaptation to climate change. However, whether agroforestry systems themselves succumb to the adverse effects of climate change is often less studied in the agroforestry literature. Consequently, less is known about how climate change will impact agroforests. We conducted a systematic review, which included an evidence quality assessment, to examine the impacts of climate change on tropical agroforestry systems (TAFS). Based primarily on studies undertaking biophysical approaches, we found that climate change negatively impacts TAFS by reducing tree growth, intensifying tree-crop resource competition and reducing crop yields. However, the impacts on smallholder farmers are less clear due to limited evidence in the relevant literature. We found that the evidence supporting our findings is mostly “robust”, although “least robust” strength evidence was also commonly found. We conclude that to improve understanding of how climate change could affect the performance of TAFS as a social ecological system, more interdisciplinary studies are required. Furthermore, to improve the quality of evidence in the research field, studies should explore using mountain elevation gradients for climate analog analysis to perform the most robust study designs. We provide an interdisciplinary conceptual model, which considers the interactions and feedbacks between TAFS components noted from our review to predict the response of ecosystem services provisioning and farmers' wellbeing to climate change, to guide interdisciplinary studies using climate analog analysis.
Joint impacts of future climate conditions and invasive species on black ash forested wetlands
Authors: Shannon Joseph , Kolka Randall , Van Grinsven Matthew , Liu Fengjing
Published: August 2022

Wetlands around the globe are being impacted by changing temperature and precipitation patterns. Simultaneously black ash forested wetlands are expected to lose much of their overstory canopy due to the invasive Emerald Ash Borer (EAB). Field experiments and modeling efforts have provided information on species tolerance of post-EAB conditions and future climate adapted species. No studies have yet examined the interaction of the loss of ash and future climate scenarios on wetland hydrologic conditions. We developed daily wetland hydrology models for three vegetation conditions: black ash forest, alternate non-ash forest, and non-forested. Model simulations were evaluated under current climate conditions and under two future climate scenarios representing warm & dry (T: +1.9°C, P: −2.6 cm) and hot & wet (T: +8.9°C, P: +6.2 cm) scenarios. For each combination of vegetation condition and climate scenario, 10,000 annual synthetic weather sequences were used as inputs to the wetland hydrology models. Simulated wetland hydrology remained highly variable based on seasonal precipitation and evaporative demand. We compared the occurrence probability of stream-network connectivity, surface inundation, and dry conditions. Effects ranged from slightly drier under non-forested and warm & dry conditions to much wetter under alternate-forested and hot & wet conditions. Non-forested conditions resulted in a median increase of 15 and 20% of daily observations of connectivity to stream networks and surface inundation, respectively, and 7% (median) fewer daily observations of dry conditions. Alternate-forested conditions resulted in larger median impacts: 40 and 35% more daily observations of connectivity to stream networks and surface inundation, respectively and 10% fewer daily observations of dry conditions. Projected climate change-induced water deficits resulted in 3–9% fewer days with connectivity and surface inundation, respectively and 0–10% more days with dry conditions (values represent the range of median values for combination of vegetation and future scenario). Our results show vegetation change as an equal or greater individual driver of future hydrologic conditions in black ash wetlands relative to climate change. Non-forested conditions and projected climate change-induced impacts each effectively negated the other. Management decisions around vegetation transition and establishment should consider the interaction with future climate scenarios and the large effect that poorly inundation-adapted plant communities could have on hydrologic conditions.
Contraceptive self-injection through routine service delivery: Experiences of Ugandan women in the public health system
Authors: Cover Jane , Namagembe Allen , Morozoff Chloe , Tumusiime Justine , Nsangi Damalie , Drake Jen Kidwell
Published: August 2022

Contraceptive self-injection (SI) is a new self-care practice with potential to transform women's family planning access by putting a popular method, injectable contraception, directly into the hands of users. Research shows that SI is feasible and acceptable; evidence regarding how to design and implement SI programs under real-world conditions is still needed. This evaluation examined women's experiences when self-injection of subcutaneous depot medroxyprogesterone acetate (DMPA-SC) was introduced in Uganda alongside other contraceptive options in the context of informed choice. We conducted structured survey interviews with 958 randomly selected SI clients trained in three districts in 2019. SI clients demonstrated their injection technique on a model to permit an assessment of injection proficiency. A randomly selected subset of 200 were re-interviewed 10–17 months post-training to understand resupply experiences, waste disposal practices and continuation. Finally, we conducted survey interviews with a random sample of 200 clients who participated in training but declined to self-inject. Data were analyzed using Stata IC/14.2. Differences between groups were measured using chi square and t-tests. Multivariate analyses predicting injection proficiency and SI adoption employed mixed effects logistic regression. Nearly three quarters of SI clients (73%) were able to demonstrate injection proficiency without additional instruction from a provider. Years of education, having received a complete training, practicing, and taking home a job aid were associated with higher odds of proficiency. Self-reported satisfaction and continuation were high, with 93% reinjecting independently 3 months post-training. However, a substantial share of those trained opted not to self-inject. Being single, having a partner supportive of family planning use, training with a job aid, practicing, witnessing a demonstration and exposure to a full training were associated with higher odds of becoming an SI client; conversely, those trained in a group had reduced odds of becoming an SI client. The self-care program was successful for the majority of women who became self-injectors, enabling most women to demonstrate SI proficiency. Nearly all those who opted to self-inject reinjected independently, and the majority continued self-injecting for at least 1 year. Additional research should identify strategies to facilitate adoption by women who wish to self-inject but face challenges.
Biodegradable nanoparticles induce cGAS/STING-dependent reprogramming of myeloid cells to promote tumor immunotherapy
Authors: Podojil Joseph R. , Cogswell Andrew C. , Chiang Ming-Yi , Eaton Valerie , Ifergan Igal , Neef Tobias , Xu Dan , Meghani Khyati A. , Yu Yanni , Orbach Sophia M. , Murthy Tushar , Boyne Michael T. , Elhofy Adam , Shea Lonnie D. , Meeks Joshua J. , Miller Stephen D.
Published: August 2022

Cancer treatment utilizing infusion therapies to enhance the patient’s own immune response against the tumor have shown significant functionality in a small subpopulation of patients. Additionally, advances have been made in the utilization of nanotechnology for the treatment of disease. We have previously reported the potent effects of 3-4 daily intravenous infusions of immune modifying poly(lactic-co-glycolic acid) (PLGA) nanoparticles (IMPs; named ONP-302) for the amelioration of acute inflammatory diseases by targeting myeloid cells. The present studies describe a novel use for ONP-302, employing an altered dosing scheme to reprogram myeloid cells resulting in significant enhancement of tumor immunity. ONP-302 infusion decreased tumor growth via the activation of the cGAS/STING pathway within myeloid cells, and subsequently increased NK cell activation via an IL-15-dependent mechanism. Additionally, ONP-302 treatment increased PD-1/PD-L1 expression in the tumor microenvironment, thereby allowing for functionality of anti-PD-1 for treatment in the B16.F10 melanoma tumor model which is normally unresponsive to monotherapy with anti-PD-1. These findings indicate that ONP-302 allows for tumor control via reprogramming myeloid cells via activation of the STING/IL-15/NK cell mechanism, as well as increasing anti-PD-1 response rates.
Evaluation of safety and immunogenicity of duck-plague virus gC/gE double gene deletion
Authors: Ruan Peilin , Feng Xin , Cheng Anchun , Wang Mingshu , Zhang Wei , Wu Ying , Yang Qiao , Tian Bin , Ou Xuming , Sun Di , Zhang Shaqiu , Mao Sai , Zhu Dekang , Jia Renyong , Chen Shun , Liu Mafeng , Zhao Xin-Xin , Huang Juan , Gao Qun , Yu Yanling , Zhang Ling , Pan Leichang
Published: August 2022

Duck plague caused by duck plague virus (DPV) is a highly contagious disease that can cause serious morbidity and death in waterfowl such as ducks and geese, and bring huge economic losses to the duck industry. In this study, on the basis of the duck plague virus gC gene deletion strain CHv-ΔgC, based on the duck plague virus bacterial artificial chromosome (BAC) platform in our laboratory, the gE gene was knocked out using the traceless deletion technology to obtain gC/gE double gene deletion candidate vaccine strain CHv-ΔgC/gE. The double gene deletion strain (CHv-ΔgC/gE) constructed in this study has greatly weakened virulence, no pathogenicity to ducks, and stable genetic characteristicsin vitroandin vivo. Ducks immunized with CHv-ΔgC/gE can produce neutralizing antibodies and ELISA antibody levels comparable to those of commercial duck plague attenuated vaccine immunization, and can resist 100 LD50CHv challenge of ducks, with good immune protection effect. It has the potential to be further developed into duck plague gC/gE double gene deletion, marked attenuated vaccine.
An integrative pan-cancer analysis revealing the difference in small ring finger family of SCF E3 ubiquitin ligases
Authors: Huang Tingting , Li Jiwei , Liu Xinli , Shi Bingbing , Li Shiqin , An Han-Xiang
Published: August 2022

Background

The SCF (Skp1-cullin-F-box proteins) complex is the largest family of E3 ubiquitin ligases that mediate multiple specific substrate proteins degradation. Two ring-finger family members RBX1/ROC1 and RBX2/RNF7/SAG are small molecular proteins necessary for ubiquitin ligation activity of the multimeric SCF complex. Accumulating evidence indicated the involvement of RBX proteins in the pathogenesis and development of cancers, but no research using pan-cancer analysis for evaluating their difference has been directed previously.

Methods

We investigated RBX1/2 expression patterns and the association with clinicopathological features, and survivals of cancer patients obtained from the TCGA pan-cancer data. The binding energies of RBX1/2-CUL1 complexes were preliminarily calculated by using molecular dynamics simulations. Meanwhile, we assessed their immune infiltration level across numerous databases, including TISIDB and Timer database.

Results

High expression levels of RBX1/2 were observed in most cancer types and correlated with poor prognosis of patients analyzed. Nonetheless, exceptions were observed: RBX2 expression in KICH was higher than normal renal tissues and played a detrimental role in KICH. The expression of RBX1 was not associated with the prognostic risk of KICH. Moreover, the combination of RBX1 and CUL1 expression is more stable than that of RBX2 and CUL1. RBX1/2 expression showed their own specific characteristics in tumor pathological stages and grades, copy number variation and immune components.

Conclusions

These findings not only indicated that the difference of RBX1/2 might result in varying degrees of tumor progression, but also suggested that they might serve as biomarkers for immune infiltration in cancers, shedding new light on therapeutics of cancers.
Tas2R signaling enhances mouse neutrophil migration via a ROCK-dependent pathway
Authors: Kobayashi Daichi , Watarai Tomoya , Ozawa Madoka , Kanda Yasuhiro , Saika Fumihiro , Kiguchi Norikazu , Takeuchi Arata , Ikawa Masahito , Matsuzaki Shinsuke , Katakai Tomoya
Published: August 2022

Type-2 bitter taste receptors (Tas2Rs) are a large family of G protein-coupled receptors that are expressed in the oral cavity and serve to detect substances with bitter tastes in foods and medicines. Recent evidence suggests that Tas2Rs are also expressed extraorally, including in immune cells. However, the role of Tas2Rs in immune cells remains controversial. Here, we demonstrate that Tas2R126, Tas2R135, and Tas2R143 are expressed in mouse neutrophils, but not in other immune cells such as macrophages or T and B lymphocytes. Treatment of bone marrow-derived neutrophils from wild-type mice with the Tas2R126/143 agonists arbutin and d-salicin led to enhanced C-X-C motif chemokine ligand 2 (CXCL2)-stimulated migration in vitro, but this response was not observed in neutrophils from Tas2r126/135/143-deficient mice. Enhancement of CXCL2-stimulated migration by Tas2R agonists was accompanied by increased phosphorylation of myosin light chain 2 (MLC2) and was blocked by pretreatment of neutrophils with inhibitors of Rho-associated coiled-coil-containing protein kinase (ROCK), but not by inhibitors of the small GTPase RhoA. Taken together, these results demonstrate that mouse neutrophils express functional Tas2R126/143 and suggest a role for Tas2R126/143–ROCK–MLC2-dependent signaling in the regulation of neutrophil migration.
The contribution of the intestinal microbiome to immune recovery after HCT
Authors: Wolfe Alex E. , Markey Kate A.
Published: August 2022

Allogenic hematopoietic stem-cell transplantation (allo-HCT) is a curative-intent immunotherapy for high-risk hematological malignancies and immune deficiencies. Allo-HCT carries a high risk of treatment-related mortality (TRM), largely due to infection or graft-versus-host disease (GVHD). Robust immune recovery is essential for optimal patient outcomes, given the immunologic graft-versus-leukemia effect prevents relapse, and functional innate and adaptive immunity are both needed for the prevention and control of infection. Most simply, we measure immune recovery by enumerating donor lymphocyte subsets in circulation. In functional terms, ideal immune recovery is more difficult to define, and current lab techniques are limited to the measurement of specific vaccine-responses or mitogens ex vivo. Clinically, poor immune function manifests as problematic infection with viral, bacterial and fungal organisms. Furthermore, the ideal recovering immune system is capable of exerting graft-versus-tumor effects to prevent relapse, and does not induce graft-versus-host disease. Large clinical observational studies have linked loss of diversity within the gut microbiome with adverse transplant outcomes including decreased overall survival and increased acute and chronic GVHD. Furthermore, the correlation between intestinal microbial communities and numeric lymphocyte recovery has now been reported using a number of approaches. Large sets of clinically available white blood cell count data, clinical flow cytometry of lymphocyte subsets and bespoke flow cytometry analyses designed to capture microbiota-specific T cells (e.g. Mucosal-associated invariant T cells, subsets of the gd T cells) have all been leveraged in an attempt to understand links between the microbiota and the recovering immune system in HCT patients. Additionally, preclinical studies suggest an immunomodulatory role for bacterial metabolites (including butyrate, secondary bile acids, and indole derivatives from tryptophan metabolism) in transplant outcomes, though further studies are needed to unravel mechanisms relevant to the post-HCT setting. An understanding of mechanistic relationships between the intestinal microbiome and post-transplant outcomes is necessary for reduction of risk associated with transplant, to inform prophylactic procedures, and ensure optimal immune reconstitution without alloreactivity. Here, we summarize the current understanding of the complex relationship between bacterial communities, their individual members, and the metabolites they produce with immune function in both the allo-HCT and steady-state setting.
Case Report: Recognition of Devastating Primary Amoebic Meningoencephalitis (PAM) Caused by Naegleria fowleri: Another Case in South China Detected via Metagenomics Next-Generation Sequencing Combined With Microscopy and a Review
Authors: Chen Shenggen , Che Chunhui , Lin Wanhui , Chen Baojian , Huang Xinming , Liu Changyun , Huang Huapin
Published: August 2022

Introduction

Primary amoebic meningoencephalitis (PAM) caused by Naegleria fowleri is seldom reported in mainland China.

Methods

One case from South China was presented, and the clinical features of the PAM, especially the early CT features, were compared to those in the literatures from PubMed/Web of Science/China National Knowledge internet (CNKI).

Case Presentation and Results

A 47-year-old man with a high fever came to the fever clinic. Twelve hours later, the man lost consciousness and exhibited generalized tonic-clonic seizures and needed ventilator-controlled ventilation. Then, he was admitted to the neurology intensive care unit (NICU). The opening pressure of his cerebrospinal fluid (CSF) was over 500 mm H2O with highly increased leukocyte/protein levels and very low glucose levels. Three days after admission, high copy numbers of Naegleria fowleri amoebae were detected by metagenomics next-generation sequencing (mNGS) and cysts were visible with wet mount microscopy. Four days after admission, the patient experienced brain death. However, the relatives of the patient did not want to give up, and he received amphotericin B (AmB). During hospitalization, he suffered from severe damage to the liver and kidneys and electrolyte disorders that required continuous renal replacement therapy (CRRT).

Review

All 20 included PAM patients suffered from fever. Seventeen of them had headache and neck stiffness. Ten of them showed generalized brain edema. To date, 7 cases of PAM have been reported in China. Only one patient survived. Most of the patients showed generalized brain edema. Only the surviving patient showed focal edema. He died three months later.

Conclusion

Rapidly progressive meningoencephalitis in which the CSF results are similar to those suffered from a bacterial infection should be considered a possible case of PAM. It can be rapidly detected with microscopy in CSF wet mounts but needs further molecular investigation for confirmation, and mNGS should be a new method used for rapid and precise identification. Moreover, CRRT may prolong the survival time of PAM patients with multiple organ failure.
Chemosynthetic and photosynthetic trophic support from cold seeps in Arctic benthic communities
Authors: Åström Emmelie K. L. , Bluhm Bodil A. , Rasmussen Tine L.
Published: August 2022

Benthic communities below the photic zone are largely reliant on the export of surface-water primary production and the flux of partially degraded organic matter to the seabed, i.e. pelagic−benthic coupling. Over the past decades, however, the role of chemosynthetically produced carbon in food webs has been recognized in various habitats. Cold seeps are now known to be widespread across circumpolar Arctic shelves where natural release of hydrocarbons occurs at the seabed. Here, we investigated to what extent chemosynthesis-based carbon (CBC) enters the food web in a high latitude shelf-system. Specifically, we estimated the contributions of chemosynthesis-based carbon to primarily benthic invertebrate taxa from seeps at both shallow and deeper shelves and comparative non-seep areas in the Svalbard-Barents Sea region using bulk stable isotope-analysis of carbon and nitrogen. Our results show low δ13C values (-51.3 to -32.7 ‰) in chemosymbiotic siboglinids and several species of benthic, higher-trophic level, invertebrates (mainly polychaetes and echinoderms; -35.0 to -26.1‰) collected at cold seeps, consistent with assimilation of chemosynthesis-based carbon into the Arctic benthic food web. Using a two-component mixing equation, we demonstrate that certain species could derive more than 50% of their carbon from chemosynthesis-based carbon. These findings show that autochthonous chemosynthetic energy sources can contribute to supporting distinct groups of ‘background’ benthic taxa at these Arctic seep-habitats beyond microbial associations and chemosymbiotic species. Furthermore, we found a higher degree of chemosynthesis-based carbon in benthos at the deeper Barents Sea shelf seeps (>330 m) compared to seeps at the Western Svalbard shelf (<150 m water-depth), and we suggest this result reflects the differences in depth range, surface production and pelagic-benthic coupling. We detected large intra-species variations in carbon signatures within and across geographical locations and, combined with isotopic niche-analysis, our results show that certain taxa that inhabits seeps, have wider trophic niches in comparison to taxa inhabiting non-seeps. The increasing number of discovered natural seeps in the Arctic suggests that chemosynthetic production from seeps could play a more critical role in Arctic trophic structure than previously thought. Seep-derived carbon should, hence, be accounted for as an additional carbon source and included in food-web and energy-flow models in future work.
Combined effect of salinity and temperature on copepod reproduction and oxidative stress in brackish-water environment
Authors: von Weissenberg Ella , Mottola Giovanna , Uurasmaa Tytti-Maria , Anttila Katja , Engström-Öst Jonna
Published: August 2022

Climate-induced warming and increased river inflows are forcing the Baltic Sea to radical changes in the near future; organisms living in this brackish-water ecosystem are already experiencing osmotic stress, which, together with thermal stress, may have severe consequences on the ecosystem. The aim of this work was to study the combined effect of decreasing salinity and increasing temperature on reproductive success and oxidative stress in zooplankton by using a calanoid copepod Acartia sp. as a model organism. The field study was conducted during summer 2020 in the western Gulf of Finland, using three sampling sites with naturally differing salinity levels. Additionally, the copepods from these sites were experimentally exposed to ambient or 3°C elevated temperature for 72 h. The copepods derived from the deepest and the most saline sampling site suffered less oxidative damage and exhibited relatively high reproduction, while the temperature treatment itself had little effect. On the other hand, the field-based monitoring data showed otherwise; temperature increased lipid peroxidation, glutathione-s-transferase activity, or both in all three sampling sites. Meanwhile, egg production rate was negatively associated with temperature in the area with the lowest salinity. Moreover, egg production rate decreased from June to September along with increasing temperatures in the mid-salinity sampling site, while similar change occurred also in the highest-salinity site between August and September. The combined effect of salinity and sampling date on reproduction indicates the importance of even subtle salinity changes on copepods. Moreover, the data suggest that the unusually strong heatwave was responsible for increased oxidative stress during the sampling season and possibly forced a trade-off between antioxidant activity and reproductive effort.
Effects of tidal variations on total nitrogen concentration, speciation, and exchange flux in the Shuidong Bay coastal water, South China Sea
Authors: Zhang Peng , Luo Weisheng , Fu Miaojian , Zhang Jibiao , Cheng Mingyue , Xie Jiale
Published: August 2022

Nitrogen (N) plays an important role in marine ecosystems as a biogenic element for phytoplankton. The tidal cycle had major influence on various biogeochemical parameters of the bay and changed nutrients input with the ebb and flow of the tide. In this study, we collected samples from Shuidong Bay (SDB) in China, in August 2021, to explore the periodic variations in coastal waters during spring and neap tides. The effects of spring and neap tides on different speciation of nitrogen in the SDB and the exchange fluxes between the SDB and the South China Sea (SCS) were investigated. The results indicated that the concentrations of particulate nitrogen (PN) and N-NO2- were significantly different between the spring and neap tides (P < 0.05). The total nitrogen (TN) concentrations in SDB during the spring and neap tides were 258.12 ± 89.49 μmol/L and 231.77 ± 56.86 μmol/L. During the spring and neap tides, total dissolved nitrogen (TDN) accounted for 54.1% and 52.2% of TN, respectively. In addition, dissolved organic nitrogen (DON) accounted for 81.4% and 69.9% of the TDN during the spring and neap tides, respectively. Furthermore, the net exchange fluxes of different speciation of nitrogen showed that the net exchange fluxes of TN were transported from SDB to SCS during the spring and neap tide, with the net exchange fluxes of 37.7 t and 8.8 t, respectively. The net exchange flux of TN during spring tide was 4.3 times higher than that of neap tide. In addition, a significant negative correlation was observed between dissolved inorganic nitrogen (DIN) and salinity in SDB during the spring and neap tides (P < 0.001), indicated that DIN was mainly influenced by terrestrial sources inputs. A significant positive correlation (P < 0.01) was observed between PN and Chlorophyll-a in SDB during the spring and neap tides, implied that the assimilation of a large number of planktonic organisms promoted PN formation. The present results revealed that tidal variation played an important role in regulating N speciation and exchange flux in coastal waters, which had great implications for N biogeochemistry and water quality improvement in SDB.
Plasticity in the morphometrics and movements of an Antarctic apex predator, the leopard seal
Authors: Kienle Sarah S. , Goebel Michael E. , LaBrecque Erin , Borras-Chavez Renato , Trumble Stephen J. , Kanatous Shane B. , Crocker Daniel E. , Costa Daniel P.
Published: August 2022

Animals that display plasticity in behavioral, ecological, and morphological traits are better poised to cope with environmental disturbances. Here, we examined individual plasticity and intraspecific variation in the morphometrics, movement patterns, and dive behavior of an enigmatic apex predator, the leopard seal (Hydrurga leptonyx). Satellite/GPS tags and time-depth recorders were deployed on 22 leopard seals off the Western Antarctic Peninsula. Adult female leopard seals were significantly larger (454±59 kg) and longer (302±11 cm) than adult males (302±22 kg, 276±11 cm). As females were 50% larger than their male counterparts, leopard seals are therefore one of the most extreme examples of female-biased sexual size dimorphism in marine mammals. Female leopard seals also spent more time hauled-out on land and ice than males. In the austral spring/summer, three adult female leopard seals hauled-out on ice for 10+ days, which likely represent the first satellite tracks of parturition and lactation for the species. While we found sex-based differences in morphometrics and haul-out durations, other variables, including maximum distance traveled and dive parameters, did not vary by sex. Regardless of sex, some leopard seals remained in near-shore habitats, traveling less than 50 kilometers, while other leopard seals traveled up to 1,700 kilometers away from the tagging location. Overall, leopard seals were short (3.0±0.7 min) and shallow (29±8 m) divers. However, within this general pattern, some individual leopard seals primarily used short, shallow dives, while others switched between short, shallow dives and long, deep dives. We also recorded the single deepest and longest dive made by any leopard seal—1, 256 meters for 25 minutes. Together, our results showcased high plasticity among leopard seals tagged in a single location. These flexible behaviors and traits may offer leopard seals, an ice-associated apex predator, resilience to the rapidly changing Southern Ocean.
A simple method for estimating macroalgae area under clouds on MODIS imagery
Authors: An Deyu , Xing Qianguo , Yu Dingfeng , Pan Shunqi
Published: August 2022

The presence of clouds interferes with optical remote sensing monitoring of macroalgae blooms. To solve this problem, we propose a simple method for estimating macroalgae area under clouds (Area_cloud_GT) on MODIS imagery using the principle behind the lowpass filter. The method is based on a rectangle with clouds and eight identical adjacent rectangles surrounding it that contain macroalgae. The cloud rectangle is a central ‘pixel’ (Cloud) and the eight adjacent rectangles are ‘pixels’ GT1–GT8. The core operation is to calculate the central ‘pixel’ value, i.e., the macroalgae coverage rate in the Cloud rectangle. The macroalgae area detected by semi-simultaneous fine resolution images in the same region was taken as the ‘real’ value. A comparison of the estimation results and the ‘real’ value shown that the mean relative difference between them (MRD) was 30.09% when the time interval of the images within 10 minutes. When the time interval was over 3 hours, the MRD was more than 60%. The MRD increased significantly with increasing time interval because of the constant movement of the macroalgae and the limitations of the remote sensing image. The results indicate that this simple method is effective to a certain extent. These results can provide a reference for the quantitative analysis of green tide.
Computed tomography enterography increases the ability of endoscopy to differentiate Crohn's disease from intestinal Behçet's disease
Authors: Yang Hong , Zhang Huimin , Liu Wei , Han Wei , Guo Tao , Lai Yamin , Tan Bei , Wang Congling , Chen Minhu , Gao Xiang , Ran Zhihua , Liu Zhanju , Wu Kaichun , Cao Qian , Qian Jiaming
Published: August 2022

Background

Distinguishing Crohn's disease (CD) and intestinal Behçet's disease (BD) is difficult in clinical practice.

Aim

To evaluate the ability of CT enterography (CTE) to enhance the diagnostic value of endoscopy in differentiating CD from intestinal BD and to establish differential diagnosis models.

Methods

A total of 113 patients with CD and 70 patients with intestinal BD from seven tertiary inflammatory bowel disease centers were enrolled. The univariate and multivariate analyses were used by SAS software version 9.2. Three differential scoring models based on the multivariate analysis of endoscopic features alone (model 1), endoscopic features combined with clinical symptoms (model 2), and endoscopic features combined with clinical symptoms and CTE (model 3) were established.

Results

The results showed that model 2 increased the efficacy of model 1 in differential diagnosis and model 3 had the highest accuracy of 84.15% at a cutoff value of two points. The scoring of model 3 was as follows: genital ulcer (−3 points), skin lesions (−3 points), oval ulcer (-2 points), longitudinal ulcer (1 point), number of ulcers > 5 (3 points), inflammatory polyps (2 points), mucosal severe enhancement (2 points), and fibrofatty proliferation (1 point).

Conclusion

Clinical symptoms and CTE increased the ability of endoscopy to differentiate CD from intestinal BD.