bluuebunny/crossref_metadata_2025 huggingface dataset:
An Online Physical Activity Intervention for Youth With Physical Disabilities: A Pilot Study
Authors: Sharma Ritu , Latimer-Cheung Amy E. , Cairney John , Arbour-Nicitopoulos Kelly P.
Published: April 2021

Background: Physical activity (PA) interventions are limited in number and reach for youth with physical disabilities (YPD) who experience systemic barriers that may preclude their in-person participation. Further, a lack of theory in the development and evaluation of PA interventions impedes our understanding and replication of active components of behavior change. These limitations pose challenges in the effective promotion of PA in YPD. Theory-based and more inclusive methods of PA intervention delivery must be explored in our efforts to promote PA and overall health in YPD.

Methods: A pilot study was conducted to evaluate the feasibility and outcomes of an online, 4-week social cognitive theory-based PA intervention for YPD. Intervention feasibility (implementation fidelity, intervention compliance, and intervention acceptability) was evaluated through manual documentation, weekly feedback questionnaires, and open-ended feedback at 1-month post-intervention. Targeted social cognitive (outcome expectations, self-efficacy [task, self-regulatory, barrier] and self-regulation) and PA behavior outcomes were self-reported at baseline and 1-week and 1-month post-intervention.

Results: Sixteen YPD (Mage = 17.4 ± 2.7 years, 69% female) completed the study. Intervention feasibility was supported by high implementation fidelity (100%), high intervention compliance (>90%), and positive ratings on indicators of acceptability for all weeks of the intervention (weekly feedback questionnaire means ranging from 5.74 to 6.19 out of 7). Through open-ended feedback, participants indicated the intervention was easy to use and understand, favorably shifted their self-awareness and personal meaning of PA, and provided value and potential for future use pertaining to the learned self-regulation skills and strategies. Participants also provided formatting and content recommendations for intervention improvement. Repeated measures ANOVAs showed significant and large effect sizes for changes in participants' task (p = 0.01, n2p = 0.28) and barrier (p = 0.02, n2p = 0.24) self-efficacy, goal-setting and planning and scheduling behaviors (ps < 0.001, n2ps = 0.42), and self-reported PA behavior (p = 0.02, n2p = 0.26).

Conclusions: An online PA intervention for YPD is feasible and may offer potential benefit through the enhancement of self-efficacy, self-regulation, and PA behavior. Continued research is necessary to understand the efficacy and longer-term outcomes of online, theory-based interventions for YPD as a PA promotion strategy.
Taking an Extended Embodied Perspective of Touch: Connection-Disconnection in iVR
Authors: Price Sara , Jewitt Carey , Chubinidze Dimitri , Barker Ned , Yiannoutsou Nikoleta
Published: April 2021

Bringing touch into VR experiences through haptics is considered increasingly important for user engagement and fostering feelings of presence and immersion, yet few qualitative studies have explored users' iVR touch experiences. This paper takes an embodied approach–bringing attention to the tactile-kinaesthetic body–to explore users' wholistic experiences of touch in iVR, moving beyond the cutaneous and tactile elements of “feeling” to elaborate upon themes of movement and kinetics. Our findings show how both touch connections and disconnections emerged though material forms of tactility (the controller, body positioning, tactile expectations) and through “felt proximities” and the tactile-kinaesthetic experience thus shaping the sense of presence. The analysis shows three key factors that influence connection and disconnection, and how connection is re-navigated or sought at moments of experienced disconnection: a sense of control or agency; identity; and bridging between the material and virtual. This extended notion of touch deepens our understanding of its role in feelings of presence by providing insight into a range of factors related to notions of touch – both physical and virtual–that come into play in creating a sense of connection or presence (e.g., histories, expectations), and highlights the potential for iVR interaction to attend to the body beyond the hands in terms of touch.
The Plausibility Paradox for Resized Users in Virtual Environments
Authors: Pouke Matti , Mimnaugh Katherine J. , Chambers Alexis P. , Ojala Timo , LaValle Steven M.
Published: April 2021

This paper identifies and confirms a perceptual phenomenon: when users interact with simulated objects in a virtual environment where the users’ scale deviates greatly from normal, there is a mismatch between the object physics they consider realistic and the object physics that would be correct at that scale. We report the findings of two studies investigating the relationship between perceived realism and a physically accurate approximation of reality in a virtual reality experience in which the user has been scaled by a factor of ten. Study 1 investigated perception of physics when scaled-down by a factor of ten, whereas Study 2 focused on enlargement by a similar amount. Studies were carried out as within-subjects experiments in which a total of 84 subjects performed simple interaction tasks with objects under two different physics simulation conditions. In thetrue physicscondition, the objects, when dropped and thrown, behaved accurately according to the physics that would be correct at that either reduced or enlarged scale in the real world. In themovie physicscondition, the objects behaved in a similar manner as they would if no scaling of the user had occurred. We found that a significant majority of the users considered themovie physicscondition to be the more realistic one. However, at enlarged scale, many users consideredtrue physicsto match their expectations even if they ultimately believedmovie physicsto be the realistic condition. We argue that our findings have implications for many virtual reality and telepresence applications involving operation with simulated or physical objects in abnormal and especially small scales.
Grazing Into the Anthropocene or Back to the Future?
Authors: Behnke Roy H.
Published: April 2021

This essay examines three central components of extensive livestock production—herd composition, grazing/pasture management, and rangeland tenure. In all of these areas, fenced, and open-range forms of migratory pastoralism face a number of shared problems. Set aside the presumption that either one of these systems is technically or institutionally more advanced than the other, and it turns out that each has lessons for the other. 1. For a variety of reasons, including climate change, we can look forward to a future world with less grass, which presents a challenge for livestock producers reliant on grass feeding livestock. With little delay and minimal scientific support, East African pastoralists are already adjusting to a new woody world by diversifying the species composition of their herds to include more browsers—camels and goats. There is a potential lesson here for commercial ranchers who have traded the stability of mixed herds for the profitability of keeping sheep or cattle alone. 2. Migratory rangeland systems distribute livestock very differently than fenced, rotational systems of livestock, and pasture management. Whereas, migratory herds exploit environmental heterogeneity, fenced ranching attempts to suppress it. Emerging archaeological evidence is demonstrating that pastoralists have amplified rangeland heterogeneity for millennia; ecological research shows that this heterogeneity sustains both plant and wildlife biodiversity at the landscape scale; and new approaches to ranch management are appropriating aspects of migratory herding for use on fenced ranches. A rapprochement between the environmental sciences, ranching, and open-range migratory pastoralism has occurred and merits wider policy recognition. 3. In contemporary Africa, indigenous tenure regimes that sustain open rangelands are eroding under pressure from market penetration and state encapsulation. At the same time in the American West, there are emerging novel land tenure instruments that replicate some of the most important functional characteristics of tenure arrangements in pastoral Africa. After many false starts, it appears that some aspects of American ranching do provide an appropriate model for the preservation of the open-range migratory systems that they were once supposed to supplant. “Development” policy needs to reflect upon this inversion of roles and its implications for accommodating diversity.
Impact of Three Different Serum Sources on Functional Properties of Equine Mesenchymal Stromal Cells
Authors: Pezzanite Lynn , Chow Lyndah , Griffenhagen Gregg , Dow Steven , Goodrich Laurie
Published: April 2021

Culture and expansion of equine mesenchymal stromal cells (MSCs) are routinely performed using fetal bovine serum (FBS) as a source of growth factors, nutrients, and extracellular matrix proteins. However, the desire to minimize introduction of xenogeneic bovine proteins or pathogens and to standardize cellular products intended for clinical application has driven evaluation of alternatives to FBS. Replacement of FBS in culture for several days before administration has been proposed to reduce antigenicity and potentially prolong survival after injection. However, the functional consequences of MSC culture in different serum types have not been fully evaluated. The objective of this study was to compare the immunomodulatory and antibacterial properties of MSCs cultured in three serum sources: FBS or autologous or allogeneic equine serum. We hypothesized that continuous culture in FBS would generate MSCs with improved functionality compared to equine serum and that there would not be important differences between MSCs cultured in autologous vs. allogeneic equine serum. To address these questions, MSCs from three healthy donor horses were expanded in medium with FBS and then switched to culture in FBS or autologous or allogeneic equine serum for 72 h. The impact of this 72-h culture period in different sera on cell viability, cell doubling time, cell morphology, bactericidal capability, chondrogenic differentiation, and production of cytokines and antimicrobial peptides was assessed. Altering serum source did not affect cell viability or morphology. However, cells cultured in FBS had shorter cell doubling times and secreted more interleukin 4 (IL-4), IL-5, IL-17, RANTES, granulocyte–macrophage colony-stimulating factor, fibroblast growth factor 2, eotaxin, and antimicrobial peptide cathelicidin/LL-37 than cells cultured in either source of equine serum. Cells cultured in FBS also exhibited greater spontaneous bactericidal activity. Notably, significant differences in any of these parameters were not observed when autologous vs. allogeneic equine serum was used for cell culture. Chondrogenic differentiation was not different between different serum sources. These results indicate that MSC culture in FBS will generate more functional cells based on a number of parameters and that the theoretical risks of FBS use in MSC culture should be weighed against the loss of MSC function likely to be incurred from culture in equine serum.
Description of the Characteristics of Five Bedding Materials and Association With Bulk Tank Milk Quality on Five New York Dairy Herds
Authors: Alanis Valeria M. , Zurakowski Michael , Pawloski Deb , Tomazi Tiago , Nydam Daryl V. , Ospina Paula A.
Published: April 2021

Environmental mastitis represents a major challenge on dairy farms where contagious pathogens are controlled by improved milking procedures. Therefore, research focused on the environment is important to improve udder health programs. The objectives of this prospective and descriptive study were to (1) describe bedding bacterial counts, pH, and dry matter (DM) of five different bedding types (organic: manure solids, straw, paper fiber; inorganic: sand, recycled sand) and (2) explore the association between bedding bacterial counts with bulk tank milk quality. This study took place within five conveniently selected commercial dairy herds, each with a predominant bedding material in lactating pens. Bedding samples (used n = 237; fresh n = 53) were collected monthly from July 2018 to July 2019 following a standard operating procedure (SOP) to minimize sampling variability. Additionally, a bulk tank (BT) milk sample (n = 40) was collected on the same day unless milk had been picked up prior to arrival. Both BT and bedding samples were submitted to the laboratory for culture and bacterial identification and quantification of Streptococcus spp, coliforms, and non-coliforms as well as detection of several pathogens of mastitis importance. Somatic cell count was evaluated in BT samples. Within bedding type, the correlation between bedding characteristics and bacterial counts in bedding was evaluated using Pearson correlation. Within bedding type, the correlation between bacterial counts in bedding samples and bacterial counts in BT were determined. The Kruskal–Wallis test was used to evaluate the bacterial count by bedding type and to evaluate BT somatic cell count differences based on bedding type. In fresh bedding, bacterial counts were generally higher for manure solids for all bacterial groups compared with other materials. In used samples, organic materials had the highest levels of all bacterial groups. The proportion of samples with detectable organisms of mastitis importance varied within and among herds in both bedding and BT samples throughout the study period. In bedding samples, a higher DM content had the lowest levels of bacterial growth compared with those with lower DM content. Most bedding samples were on the alkaline side within a pH range of 8–11. No relationship between bacterial counts and pH was observed. No associations between BT bacteria counts and bedding bacterial counts were observed. No association between bulk tank somatic cell counts based on bedding type were observed. Despite using an SOP for bedding sampling in an effort to consistently collect samples, we still observed a large amount of variability both within and among bedding samples. This variability may have obscured any potential association between BT milk quality and bedding type.
Evaluation of Chromogenic Culture Media for Rapid Identification of Gram-Positive Bacteria Causing Mastitis
Authors: Garcia Breno Luis Nery , Fidelis Carlos Eduardo , Freu Gustavo , Granja Brunna de Mattos , dos Santos Marcos Veiga
Published: April 2021

The present study aimed to evaluate the diagnostic performance specificity (Sp), sensitivity (Se), positive predictive value (PPV), negative predictive value (NPV), and accuracy (Acc) of two chromogenic culture media for rapid identification of Gram-positive bacteria causing subclinical mastitis (SCM) in dairy cows. For this, the performance of chromogenic culture media Gram-positive (GP) and Staphylococcus (Staph) (CHROMagar ™, Paris—France) was evaluated in milk samples collected from: (1) lactating cows with SCM (n = 504), and (2) cows in the post-partum period (PP) (7 ± 3 days post-partum; n = 536). Rapid identification of Gram-positive bacteria in chromogenic media was performed by visual inspection of colony colors after 24 h of incubation at 37°C. Bacterial identification by MALDI-TOF mass spectrometry was considered the reference methodology for calculating: Acc, Se, Sp, PPV, NPV, and Cohen's Kappa coefficient of agreement (k). The chromogenic media GP showed high Acc for Strep. agalactiae/dysgalactiae identification in both samples of SCM (Se: 89.1%; Sp: 96.3% and Acc: 95.6%) and of cows in PP (Se: 100%; Sp: 99.0% and Acc: 99.1%). Similar results were observed for Strep. uberis/Enterococcus spp. identification (Se: 90.5%; Sp: 92.5% and Acc: 92.3%) in SCM samples and Se: 100%; Sp: 99.6% and Acc: 99.6% in samples of PP cows using the GP media. However, the GP chromogenic media showed low Se (25.0% in SCM samples and 50.0% in samples of cows in PP) for Staph. aureus identification, despite Sp and Acc were high (Sp: 98.3% and Acc: 95.4% in SCM and Sp samples: 99.4% and Acc: 98.9% in PP cow samples). Staph culture media showed high Acc for Staph. aureus identification (Se: 80.0%; Sp: 98.8% and Acc: 98.0% in SCM samples and Se: 66.7%; Sp: 100% and Acc: 99.6% in PP cow samples), although the low prevalence of Staph. epidermidis and Staph. saprophyticus limit inferences about the performance of identifying these pathogens in Staph media. In conclusion, despite the limitation of the GP media for identification of Staph. aureus, GP, and Staph chromogenic media obtained satisfactory diagnostic performance results for the rapid identification of the main Gram-positive pathogens associated with SCM.
Circulating Survivin Protein Levels in Lung Cancer Patients Treated With Platinum-Based Chemotherapy
Authors: Puskas Rita , Bikov Andras , Horvath Peter , Lazar Zsofia , Kunos Laszlo , Nagy Reka , Pinter Gabriella , Galffy Gabriella
Published: April 2021

The survivin protein contributes to the development and progression of tumors. Protein expression and mRNA levels correlate with clinicopathological parameters and survival of cancer patients. Our purpose was to evaluate whether circulating survivin levels have any diagnostic or predictive value in lung cancer. 118 patients with advanced stage lung cancer participated in our study. 53 suffered from adenocarcinoma (ADC), 33 from squamous cell carcinoma (SqCC), and 32 from small cell lung cancer (SCLC). We also enrolled 21 control subjects. Blood samples were collected before and after two cycles of chemotherapy. We measured survivin concentrations with ELISA. Non-parametric tests were used for analysis. We did not find significant difference in survivin levels between patients and control subjects (17.19/0–829.74/vs. 49.13/0–165.92/pg/ml; p = 0.07). We found lower survivin concentrations in patients with SqCC (0/0–171.24/pg/ml) than in those with ADC (24.94/0–626.46 pg/ml) and SCLC (45.51/0–829.74/pg/ml) (ADC vs. SqCC p < 0.0001, ADC vs. SCLC p = 0.0405, SqCC vs. SCLC p < 0.0001). Survivin levels were higher in stage IV patients than in patients without distant metastases (p = 0.0061), and concentrations were progressively higher with increasing number of metastatic organ sites (p = 0.04). We observed a decrease in survivin levels in ADC patients after platinum plus pemetrexed chemotherapy (26.22/0–626.46/pg/ml before vs. 0/0–114.36/pg/ml after; p = 0.01). Neither progression-free nor overall survival correlated with survivin levels at baseline. Our data imply that survivin may be involved in the development of metastases and it might be used as a biomarker of disease progression. However, circulating survivin concentrations do not predict survival of patients with lung cancer.
Effects of Heat Stress on Gut-Microbial Metabolites, Gastrointestinal Peptides, Glycolipid Metabolism, and Performance of Broilers
Authors: Wang Guangju , Li Xiumei , Zhou Ying , Feng Jinghai , Zhang Minhong
Published: April 2021

This paper investigated the effects of heat stress on gut-microbial metabolites, gastrointestinal peptides, glycolipid metabolism, and performance of broilers. Thus, 132 male Arbor Acres broilers, 28-days-old, were randomly distributed to undergo two treatments: thermoneutral control (TC, 21 °C) and high temperature (HT, 31 °C). The results showed that the average daily gain (ADG), average daily feed intake (ADFI), and gastric inhibitory polypeptide (GIP) concentration in the jejunum significantly decreased the core temperature, feed conversion ratio (FCR), and ghrelin of the hypothalamus, and cholecystokinin (CCK) in jejunum, and serum significantly increased in the HT group (p < 0.05). Exploration of the structure of cecal microbes was accomplished by sequencing 16S rRNA genes. The sequencing results showed that the proportion of Christensenellaceae and Lachnospiraceae decreased significantly whereas the proportion of Peptococcaceae increased at the family level (p < 0.05). Ruminococcus and Clostridium abundances significantly increased at the genus level. Furthermore, the content of acetate in the HT group significantly increased. Biochemical parameters showed that the blood glucose concentration of the HT group significantly decreased, and the TG (serum triglycerides), TC (total cholesterol), insulin concentration, and the insulin resistance index significantly increased. Nonesterified fatty acid (NEFA) in the HT group decreased significantly. In conclusion, the results of this paper suggest that the poor production performance of broilers under heat stress may be related to short-chain fatty acids (SCFAs) fermented by intestinal microbiota involved in regulating metabolic disorders.
Evaluation of TiO2 Based Photocatalytic Treatment of Odor and Gaseous Emissions from Swine Manure with UV-A and UV-C
Authors: Lee Myeongseong , Koziel Jacek A. , Murphy Wyatt , Jenks William S. , Chen Baitong , Li Peiyang , Banik Chumki
Published: April 2021

It is essential to mitigate gaseous emissions that result from poultry and livestock production to increase industry sustainability. Odorous volatile organic compounds (VOCs), ammonia (NH3), hydrogen sulfide (H2S), and greenhouse gases (GHGs) have detrimental effects on the quality of life in rural communities, the environment, and climate. This study’s objective was to evaluate the photocatalytic UV treatment of gaseous emissions of odor, odorous VOCs, NH3, and other gases (GHGs, O3—sometimes considered as by-products of UV treatment) from stored swine manure on a pilot-scale. The manure emissions were treated in fast-moving air using a mobile lab equipped with UV-A and UV-C lights and TiO2-based photocatalyst. Treated gas airflow (0.25–0.76 m3∙s−1) simulates output from a small ventilation fan in a barn. Through controlling the light intensity and airflow, UV dose was tested for techno-economic analyses. The treatment effectiveness depended on the UV dose and wavelength. Under UV-A (367 nm) photocatalysis, the percent reduction of targeted gases was up to (i) 63% of odor, (ii) 51%, 51%, 53%, 67%, and 32% of acetic acid, propanoic acid, butanoic acid, p-cresol, and indole, respectively, (iii) 14% of nitrous oxide (N2O), (iv) 100% of O3, and 26% generation of CO2. Under UV-C (185 + 254 nm) photocatalysis, the percent reductions of target gases were up to (i) 54% and 47% for p-cresol and indole, respectively, (ii) 25% of N2O, (iii) 71% of CH4, and 46% and 139% generation of CO2 and O3, respectively. The results proved that the UV technology was sufficiently effective in treating odorous gases, and the mobile lab was ready for farm-scale trials. The UV technology can be considered for the scaled-up treatment of emissions and air quality improvement inside livestock barns. Results from this study are needed to inform the experimental design for future on-farm research with UV-A and UV-C.
Hyperketonemia Predictions Provide an On-Farm Management Tool with Epidemiological Insights
Authors: Pralle Ryan S. , Amdall Joel D. , Fourdraine Robert H. , Oetzel Garrett R. , White Heather M.
Published: April 2021

Prediction of hyperketonemia (HYK), a postpartum metabolic disorder in dairy cows, through use of cow and milk data has allowed for high-throughput detection and monitoring during monthly milk sampling. The objective of this study was to determine associations between predicted HYK (pHYK) and production parameters in a dataset generated from routine milk analysis samples. Data from 240,714 lactations across 335 farms were analyzed with multiple linear regression models to determine HYK status. Data on HYK or disease treatment was not solicited. Consistent with past research, pHYK cows had greater previous lactation dry period length, somatic cell count, and dystocia. Cows identified as pHYK had lower milk yield and protein percent but greater milk fat, specifically greater mixed and preformed fatty acids (FA), and greater somatic cell count (SCC). Differential somatic cell count was greater in second and fourth parity pHYK cows. Culling (60d), days open, and number of artificial inseminations were greater in pHYK cows. Hyperketonemia prevalence decreased linearly in herds with greater rolling herd average milk yield. This research confirms previously identified risk factors and negative outcomes associated with pHYK and highlights novel associations with differential SCC, mixed FA, and preformed FA across farm sizes and production levels.
Economic Analysis of the Use of VCS2000 for Pork Carcass Meat Yield Grading in Korea
Authors: Kim Juntae , Han Hyo-Dong , Lee Wang Yeol , Wakholi Collins , Lee Jayoung , Jeong Youn-Bok , Bae Jeong Hwan , Cho Byoung-Kwan
Published: April 2021

Currently, the pork industry is incorporating in-line automation with the aim of increasing the slaughtered pork carcass throughput while monitoring quality and safety. In Korea, 21 parameters (such as back-fat thickness and carcass weight) are used for quality grading of pork carcasses. Recently, the VCS2000 system—an automatic meat yield grading machine system—was introduced to enhance grading efficiency and therefore increase pork carcass production. The VCS2000 system is able to predict pork carcass yield based on image analysis. This study also conducted an economic analysis of the system using a cost—benefit analysis. The subsection items of the cost-benefit analysis considered were net present value (NPV), internal rate of return (IRR), and benefit/cost ratio (BC ratio), and each method was verified through sensitivity analysis. For our analysis, the benefits were grouped into three categories: the benefits of reducing labor costs, the benefits of improving meat yield production, and the benefits of reducing pig feed consumption through optimization. The cost-benefit analysis of the system resulted in an NPV of approximately 615.6 million Korean won, an IRR of 13.52%, and a B/C ratio of 1.65.
An Investigation into the Impact of Pre-Adolescent Training on Canine Behavior
Authors: Dinwoodie Ian R. , Zottola Vivian , Dodman Nicholas H.
Published: April 2021

An online survey about puppy training was sent to members of the Center for Canine Behavior Studies and posted on our social media platforms. Six hundred forty-one (641) qualifying owners provided information on 1023 dogs. About half (48%) of the dogs involved in the study attended puppy training and the balance (52%) did not. The goal of the study was to find out whether puppy training at various ages (1–3 months, 4 months, 5–6 months) helped prevent behavior problems later in life (≥1 year). Attending training at 6 months of age or younger resulted in 0.71 the odds of developing aggressive behavior (95% CI: 0.53–0.97; p = 0.030), 0.64 the odds of having a compulsive behavior (95% CI: 0.45–0.92; p = 0.015), 0.60 the odds of exhibiting destructive behavior (95% CI: 0.37–0.96; p = 0.035), 0.68 the odds of excessive barking (95% CI: 0.47–0.99; p = 0.043), and 1.56 the odds of house soiling (95% CI: 1.08–2.27; p = 0.019). Ancillary findings about the entire study population were that dogs acquired at 12 weeks of age or younger were found to have 0.65 the odds of fear/anxiety (95% CI: 0.46–0.92; p = 0.016) and 0.50 the odds of exhibiting destructive behavior (95% CI: 0.31–0.79; p = 0.003). In addition, male dogs were found to have 0.68 the odds of developing aggressive behavior (95% CI: 0.53–0.88; p = 0.003), 0.66 the odds of developing compulsive behavior (95% CI: 0.49–0.88; p = 0.006), 0.37 the odds of mounting/humping (95% CI: 0.26–0.52; p < 0.001), and 1.53 the odds of rolling in repulsive materials (95% CI: 1.18–1.97; p = 0.001). Neutered dogs of either sex were found to have 3.10 the odds of fear/anxiety (95% CI: 2.05–4.72; p < 0.001), 1.97 the odds of escaping/running away (95% CI: 1.12–3.69; p = 0.025), 2.01 the odds of exhibiting coprophagia (95% CI 1.30–3.19; p = 0.002), and 1.72 the odds of rolling in repulsive materials (95% CI: 1.12–2.66; p = 0.014). The odds of problematic jumping deceased by 0.84 for each 1-year increase in age (95% CI: 0.80–0.88; p < 0.001).
ESBL-Producing Escherichia coli Carrying CTX-M Genes Circulating among Livestock, Dogs, and Wild Mammals in Small-Scale Farms of Central Chile
Authors: Benavides Julio A. , Salgado-Caxito Marília , Opazo-Capurro Andrés , González Muñoz Paulina , Piñeiro Ana , Otto Medina Macarena , Rivas Lina , Munita Jose , Millán Javier
Published: April 2021

Antibiotic-resistant bacteria of critical importance for global health such as extended-spectrum beta-lactamases-producing (ESBL)-Escherichia coli have been detected in livestock, dogs, and wildlife worldwide. However, the dynamics of ESBL-E. coli between these animals remains poorly understood, particularly in small-scale farms of low and middle-income countries where contact between species can be frequent. We compared the prevalence of fecal carriage of ESBL-E. coli among 332 livestock (207 cows, 15 pigs, 60 horses, 40 sheep, 6 goats, 4 chickens), 82 dogs, and wildlife including 131 European rabbits, 30 rodents, and 12 Andean foxes sharing territory in peri-urban localities of central Chile. The prevalence was lower in livestock (3.0%) and wildlife (0.5%) compared to dogs (24%). Among 47 ESBL-E. coli isolates recovered, CTX-M-group 1 was the main ESBL genotype identified, followed by CTX-M-groups 2, 9, 8, and 25. ERIC-PCR showed no cluster of E. coli clones by either host species nor locality. To our knowledge, this is the first report of ESBL-E. coli among sheep, cattle, dogs, and rodents of Chile, confirming their fecal carriage among domestic and wild animals in small-scale farms. The high prevalence of ESBL-E. coli in dogs encourages further investigation on their role as potential reservoirs of this bacteria in agricultural settings.
Catalase Protects Biofilm of Staphylococcus aureus against Daptomycin Activity
Authors: El Haj Cristina , Lichtenberg Mads , Nielsen Karen Leth , Bjarnsholt Thomas , Jensen Peter Østrup
Published: April 2021

Daptomycin is recommended for the treatment of Staphylococcus aureus infections due to its bactericidal activity. However, its mechanism of action is poorly understood. The involvement of reactive oxygen species (ROS) in the bactericidal activity of daptomycin has been proved against planktonic S. aureus, but not against the biofilm of S. aureus. Therefore, we evaluated if ROS contributes to the effect of daptomycin against biofilm of S. aureus. Biofilms of wild type, catalase deficient and daptomycin-resistant S. aureus strains were grown in microtiter-plates. After three days, the biofilms were exposed to daptomycin with or without thiourea in the presence of a ROS indicator. After overnight incubation, the amount of ROS and the percentage of surviving bacteria were determined. The bacterial survival was higher and the amount of ROS was lower in the wild type than in the catalase deficient biofilm, demonstrating a protective effect of catalase against daptomycin. The induction of cytotoxic ROS formation by daptomycin was verified by the addition of thiourea, which reduced the amount of ROS and protected the wild type biofilm against high concentrations of daptomycin. Accordingly, only the highest concentration of daptomycin reduced the bacterial survival and increased the ROS formation in the resistant biofilm. In conclusion, daptomycin induced the production of cytotoxic levels of endogenous ROS in S. aureus biofilm and the presence of catalase protected the biofilm against the lethality of the induced ROS.
Recent Advances on Nanoparticle Based Strategies for Improving Carotenoid Stability and Biological Activity
Authors: Sridhar Kandi , Inbaraj Baskaran Stephen , Chen Bing-Huei
Published: April 2021

Carotenoids are natural pigments widely used in food industries due to their health-promoting properties. However, the presence of long-chain conjugated double bonds are responsible for chemical instability, poor water solubility, low bioavailability and high susceptibility to oxidation. The application of a nanoencapsulation technique has thus become a vital means to enhance stability of carotenoids under physiological conditions due to their small particle size, high aqueous solubility and improved bioavailability. This review intends to overview the advances in preparation, characterization, biocompatibility and application of nanocarotenoids reported in research/review papers published in peer-reviewed journals over the last five years. More specifically, nanocarotenoids were prepared from both carotenoid extracts and standards by employing various preparation techniques to yield different nanostructures including nanoemulsions, nanoliposomes, polymeric/biopolymeric nanoparticles, solid lipid nanoparticles, nanostructured lipid nanoparticles, supercritical fluid-based nanoparticles and metal/metal oxide nanoparticles. Stability studies involved evaluation of physical stability and/or chemical stability under different storage conditions and heating temperatures for varied lengths of time, while the release behavior and bioaccessibility were determined by various in vitro digestion and absorption models as well as bioavailability through elucidating pharmacokinetics in an animal model. Moreover, application of nanocarotenoids for various biological applications including antioxidant, anticancer, antibacterial, antiaging, cosmetics, diabetic wound healing and hepatic steatosis were summarized.
Effects of Vehicle Type and Inter-Vehicle Distance on Aerodynamic Characteristics during Vehicle Platooning
Authors: Kim Wootaek , Noh Jongchan , Lee Jinwook
Published: April 2021

Considering the future development in vehicle platooning technology and the multiple models pertaining to complex road environments involving freight cars and general vehicles, the speed and distance of a vehicle model were set as variables in this study. This study aimed at analyzing the effect of currents acting differently using SolidWorks Flow Simulation tool for the vehicle platooning between different models of trucks that are currently being studied actively and sports utility vehicle (SUV) whose market share has been increasing, in order to evaluate the changes in the drag coefficient and their causes. Additionally, purpose-based vehicle (PBV) presented by Hyundai Motor (Ulsan, Korea) during the CES 2020 was considered. In this study, we found that the shape of the rear side of the leading vehicle reduces the drag coefficient of the following vehicle by washing the wake, similar to a spoiler at the rear. The rear side area of the leading vehicle forms a wide range of low pressures, which increases the drag coefficient effect of the following vehicle. The overall height of the leading vehicle also generates a distribution of low pressures above the rear of the vehicle. This reduces the impact of low pressures on the overall height of the following vehicle. The shape of the front of the following vehicle enables the wake of the leading vehicle, which involves low pressures, to inhibit the Bernoulli effect of the following vehicle. Furthermore, the front of the following vehicle continues to be affected by the wake of the leading vehicle, resulting in an increase in the drag coefficient reduction.
Modification of a Solar Thermal Collector to Promote Heat Transfer inside an Evacuated Tube Solar Thermal Absorber
Authors: Supankanok Rasa , Sriwong Sukanpirom , Ponpo Phisan , Wu Wei , Chandra-ambhorn Walairat , Anantpinijwatna Amata
Published: April 2021

Evacuated-tube solar collector (ETSC) is developed to achieve high heating medium temperature. Heat transfer fluid contained inside a copper heat pipe directly affects the heating medium temperature. A 10 mol% of ethylene-glycol in water is the heat transfer fluid in this system. The purpose of this study is to modify inner structure of the evacuated tube for promoting heat transfer through aluminum fin to the copper heat pipe by inserting stainless-steel scrubbers in the evacuated tube to increase heat conduction surface area. The experiment is set up to measure the temperature of heat transfer fluid at a heat pipe tip which is a heat exchange area between heat transfer fluid and heating medium. The vapor/ liquid equilibrium (VLE) theory is applied to investigate phase change behavior of the heat transfer fluid. Mathematical model validated with 6 experimental results is set up to investigate the performance of ETSC system and evaluate the feasibility of applying the modified ETSC in small-scale industries. The results indicate that the average temperature of heat transfer fluid in a modified tube increased to 160.32 °C which is higher than a standard tube by approximately 22 °C leading to the increase in its efficiency by 34.96%.
The Evolution of Fabrication Methods in Human Retina Regeneration
Authors: Belgio Beatrice , Salvetti Anna Paola , Mantero Sara , Boschetti Federica
Published: April 2021

Optic nerve and retinal diseases such as age-related macular degeneration and inherited retinal dystrophies (IRDs) often cause permanent sight loss. Currently, a limited number of retinal diseases can be treated. Hence, new strategies are needed. Regenerative medicine and especially tissue engineering have recently emerged as promising alternatives to repair retinal degeneration and recover vision. Here, we provide an overview of retinal anatomy and diseases and a comprehensive review of retinal regeneration approaches. In the first part of the review, we present scaffold-free approaches such as gene therapy and cell sheet technology while in the second part, we focus on fabrication techniques to produce a retinal scaffold with a particular emphasis on recent trends and advances in fabrication techniques. To this end, the use of electrospinning, 3D bioprinting and lithography in retinal regeneration was explored.
A BMI Based on Motor Imagery and Attention for Commanding a Lower-Limb Robotic Exoskeleton: A Case Study
Authors: Ferrero Laura , Quiles Vicente , Ortiz Mario , Iáñez Eduardo , Azorín José M.
Published: April 2021

Lower-limb robotic exoskeletons are wearable devices that can be beneficial for people with lower-extremity motor impairment because they can be valuable in rehabilitation or assistance. These devices can be controlled mentally by means of brain–machine interfaces (BMI). The aim of the present study was the design of a BMI based on motor imagery (MI) to control the gait of a lower-limb exoskeleton. The evaluation is carried out with able-bodied subjects as a preliminary study since potential users are people with motor limitations. The proposed control works as a state machine, i.e., the decoding algorithm is different to start (standing still) and to stop (walking). The BMI combines two different paradigms for reducing the false triggering rate (when the BMI identifies irrelevant brain tasks as MI), one based on motor imagery and another one based on the attention to the gait of the user. Research was divided into two parts. First, during the training phase, results showed an average accuracy of 68.44 ± 8.46% for the MI paradigm and 65.45 ± 5.53% for the attention paradigm. Then, during the test phase, the exoskeleton was controlled by the BMI and the average performance was 64.50 ± 10.66%, with very few false positives. Participants completed various sessions and there was a significant improvement over time. These results indicate that, after several sessions, the developed system may be employed for controlling a lower-limb exoskeleton, which could benefit people with motor impairment as an assistance device and/or as a therapeutic approach with very limited false activations.
Health Indicators Construction and Remaining Useful Life Estimation for Concrete Structures Using Deep Neural Networks
Authors: Tra Viet , Nguyen Tuan-Khai , Kim Cheol-Hong , Kim Jong-Myon
Published: April 2021

Remaining useful life (RUL) prognosis is one of the most important techniques in concrete structure health management. This technique evaluates the concrete structure strength through determining the advent of failure, which is very helpful to reduce maintenance costs and extend structure life. Degradation information with the capability of reflecting structure health can be considered as a principal factor to achieve better prognosis performance. In traditional data-driven RUL prognosis, there are drawbacks in which features are manually extracted and threshold is defined to mark the specimen’s breakdown. To overcome these limitations, this paper presents an innovative SAE-DNN structure capable of automatic health indicator (HI) construction from raw signals. HI curves constructed by SAE-DNN have much better fitness metrics than HI curves constructed from statistical parameters such as RMS, Kurtosis, Sknewness, etc. In the next stage, HI curves constructed from training degradation data are then used to train a long short-term memory recurrent neural network (LSTM-RNN). The LSTM-RNN is utilized as a RUL predictor since its special gates allow it to learn long-term dependencies even when the training data is limited. Model construction, verification, and comparison are performed on experimental reinforced concrete (RC) beam data. Experimental results indicates that LSTM-RNN generally estimates more accurate RULs of concrete beams than GRU-RNN and simple RNN with the average prediction error cycles was less than half compared to those of the simple RNN.
Intermolar Mandibular Distraction Osteogenesis—A Preliminary Report
Authors: Lie Suen A. N. , Engelen Britt H. B. T. , Timmer Veronique C. M. L. , Vrijens Nico M. P. , Asperio Paolo , Kessler Peter A. W. H.
Published: April 2021

Background: Dental Class II is the most common indication for combined orthodontic-orthognathic treatment. Intermolar mandibular distraction osteogenesis (IMDO) treatment was performed during the growth spurt, to avoid surgery at a later age. The aim of this study is to present our first experience with IMDO. Methods: This is a retrospective case series of patients who underwent an IMDO. All patients showed mandibular retrognathism, and orthodontic treatment with functional appliances was not successful. Results: In total, 20 patients (mean age of 14.8 years (SD = 0.9 ys) were included. All patients achieved a Class I occlusion. An average length gain of 9.6 mm (SD = 3.7 mm) was reached. In one patient an abscess occurred. Nine patients presented with root fractures of the second molar; three were lost, one treated endodontically. The average time between insertion and removal of the distractors was 4.6 months (SD = 1.5 mths). In one case a premature consolidation was seen. Conclusion: We achieved satisfactory results with IMDO, although undesirable effects occurred. An advantage is the manageable overall treatment time. Open questions concern the occurrence of root fractures. Furthermore, the question of long-term stability is open. The question of dynamic distraction treatment in relation to temporomandibular joint changes can only be answered in the long term.
Using a Process Approach to Pandemic Planning: A Case Study
Authors: Tomaskova Hana , Tirkolaee Erfan Babaee
Published: April 2021

The purpose of this article was to demonstrate the difference between a pandemic plan’s textual prescription and its effective processing using graphical notation. Before creating a case study of the Business Process Model and Notation (BPMN) of the Czech Republic’s pandemic plan, we conducted a systematic review of the process approach in pandemic planning and a document analysis of relevant public documents. The authors emphasized the opacity of hundreds of pages of text records in an explanatory case study and demonstrated the effectiveness of the process approach in reengineering and improving the response to such a critical situation. A potential extension to the automation and involvement of SMART technologies or process optimization through process mining techniques is presented as a future research topic.
Determination of the Vertical Load on the Carrying Structure of a Flat Wagon with the 18–100 and Y25 Bogies
Authors: Fomin Oleksij , Lovska Alyona , Píštěk Václav , Kučera Pavel
Published: April 2021

The study deals with determination of the vertical load on the carrying structure of a flat wagon on the 18–100 and Y25 bogies using mathematic modelling. The study was made for an empty wagon passing over a joint irregularity. The authors calculated the carrying structure of a flat wagon with the designed parameters and the actual features recorded during field tests. The mathematical model was solved in MathCad software. The study found that application of the Y25 bogie for a flat wagon with the designed parameters can decrease the dynamic load by 41.1% in comparison to that with the 18–100 bogie. Therefore, application of the Y25 bogie under a flat wagon with the actual parameters allows decreasing the dynamic loading by 41.4% in comparison to that with the 18–100 bogie. The study also looks at the service life of the supporting structure of a flat wagon with the Y25 bogie, which can be more than twice as long as the 18–100 bogie. The research can be of interest for specialists concerned with improvements in the dynamic characteristics and the fatigue strength of freight cars, safe rail operation, freight security, and the results of the research can be used for development of innovative wagon structures.
Emotion Recognition: An Evaluation of ERP Features Acquired from Frontal EEG Electrodes
Authors: Singh Moon Inder , Singh Mandeep
Published: April 2021

The challenge to develop an affective Brain Computer Interface requires the understanding of emotions psychologically, physiologically as well as analytically. To make the analysis and classification of emotions possible, emotions have been represented in a two-dimensional or three-dimensional space represented by arousal and valence domains or arousal, valence and dominance domains, respectively. This paper presents the classification of emotions into four classes in an arousal–valence plane using the orthogonal nature of emotions. The average Event Related Potential (ERP) attributes and differential of average ERPs acquired from the frontal region of 24 subjects have been used to classify emotions into four classes. The attributes acquired from the frontal electrodes, viz., Fp1, Fp2, F3, F4, F8 and Fz, have been used for developing a classifier. The four-class subject-independent emotion classification results in the range of 67–83% have been obtained. Using three classifiers, a mid-range accuracy of 85% has been obtained, which is considerably better than existing studies on ERPs.
Evaluation of Changes in Structure of Modified Cement Composite Using Fractal Analysis
Authors: Yakovlev Grigory , Polyanskikh Irina , Belykh V. , Stepanov Vladimir , Smirnova Olga
Published: April 2021

The need for research using modern methods of physical and chemical analysis in combination with methods for processing the graphical results arises with an increase of developments aimed at changing the structure and properties of hydration-hardening materials. Currently, the method of scanning electron microscopy is widely used for the qualitative and quantitative analysis of materials. Additional information, including fractal analysis of the image, determines the information-entropy levels and the state of the information system in the form of the microstructure based on the generalized structural entropy that increases the volume and quality of analytical information extracted from the obtained images. Images with a magnification of 20,000 times were used to analyze the microstructure of a control cement composite and a carbon black modified composite. The fractal analysis of the images revealed a change in the relative organization of the system, which justifiedthe nature of the change in the physical and technical properties of the modified cement composite relative to the additive-free cement composite from the perspective of self-organization.