bluuebunny/crossref_metadata_2025 huggingface dataset:
Transcriptomic Profiles of CD47 in Breast Tumors Predict Outcome and Are Associated with Immune Activation
Authors: Noblejas-López María del Mar , Baliu-Piqué Mariona , Nieto-Jiménez Cristina , Cimas Francisco J. , Morafraile Esther C. , Pandiella Atanasio , Corbi Ángel L. , Győrffy Balázs , Ocaña Alberto
Published: April 2021

Targeting the innate immune system has attracted attention with the development of anti- CD47 antibodies. Anti-CD47 antibodies block the inhibition of the phagocytic activity of macrophages caused by the up-regulation of CD47 on tumor cells. In this study, public genomic data was used to identify genes highly expressed in breast tumors with elevated CD47 expression and analyzed the association between the presence of tumor immune infiltrates and the expression of the selected genes. We found that 142 genes positively correlated with CD47, of which 83 predicted favorable and 32 detrimental relapse-free survival (RFS). From those associated with favorable RFS, we selected the genes with immunologic biological functions and defined a CD47-immune signature composed of PTPRC, HLA-E, TGFBR2, PTGER4, ETS1, and OPTN. In the basal-like and HER2+ breast cancer subtypes, the expression of the CD47-immune signature predicted favorable outcome, correlated with the presence of tumor immune infiltrates, and with gene expression signatures of T cell activation. Moreover, CD47 up-regulated genes associated with favorable survival correlated with pro-tumoral macrophages. In summary, we described a CD47-immune gene signature composed of 6 genes associated with favorable prognosis, T cell activation, and pro-tumoral macrophages in breast cancer tumors expressing high levels of CD47.
Role of Oxidative DNA Damage and Repair in Atrial Fibrillation and Ischemic Heart Disease
Authors: Hu Liangyu , Wang Zhengkun , Carmone Claudia , Keijer Jaap , Zhang Deli
Published: April 2021

Atrial fibrillation (AF) and ischemic heart disease (IHD) represent the two most common clinical cardiac diseases, characterized by angina, arrhythmia, myocardial damage, and cardiac dysfunction, significantly contributing to cardiovascular morbidity and mortality and posing a heavy socio-economic burden on society worldwide. Current treatments of these two diseases are mainly symptomatic and lack efficacy. There is thus an urgent need to develop novel therapies based on the underlying pathophysiological mechanisms. Emerging evidence indicates that oxidative DNA damage might be a major underlying mechanism that promotes a variety of cardiac diseases, including AF and IHD. Antioxidants, nicotinamide adenine dinucleotide (NAD+) boosters, and enzymes involved in oxidative DNA repair processes have been shown to attenuate oxidative damage to DNA, making them potential therapeutic targets for AF and IHD. In this review, we first summarize the main molecular mechanisms responsible for oxidative DNA damage and repair both in nuclei and mitochondria, then describe the effects of oxidative DNA damage on the development of AF and IHD, and finally discuss potential targets for oxidative DNA repair-based therapeutic approaches for these two cardiac diseases.
Early Postnatal Cardiac Stress Does Not Influence Ventricular Cardiomyocyte Cell-Cycle Withdrawal
Authors: Günthel Marie , van Duijvenboden Karel , Jeremiasse Jorn , van den Hoff Maurice J. B. , Christoffels Vincent M.
Published: April 2021

Congenital heart disease (CHD) is the most common birth defect. After birth, patients with CHD may suffer from cardiac stress resulting from abnormal loading conditions. However, it is not known how this cardiac burden influences postnatal development and adaptation of the ventricles. To study the transcriptional and cell-cycle response of neonatal cardiomyocytes to cardiac stress, we used a genetic mouse model that develops left ventricular volume overload within 2 weeks after birth. The increased volume load caused upregulation of the cardiac stress marker Nppa in the left ventricle and interventricular septum as early as 12 days after birth. Transcriptome analysis revealed that cardiac stress induced the expression of cell-cycle genes. This did not influence postnatal cell-cycle withdrawal of cardiomyocytes and other cell types in the ventricles as measured by Ki-67 immunostaining.
Neuropathology of the Brainstem to Mechanistically Understand and to Treat Alzheimer’s Disease
Authors: Patthy Ágoston , Murai János , Hanics János , Pintér Anna , Zahola Péter , Hökfelt Tomas G. M. , Harkany Tibor , Alpár Alán
Published: April 2021

Alzheimer’s disease (AD) is a devastating neurodegenerative disorder as yet without effective therapy. Symptoms of this disorder typically reflect cortical malfunction with local neurohistopathology, which biased investigators to search for focal triggers and molecular mechanisms. Cortex, however, receives massive afferents from caudal brain structures, which do not only convey specific information but powerfully tune ensemble activity. Moreover, there is evidence that the start of AD is subcortical. The brainstem harbors monoamine systems, which establish a dense innervation in both allo- and neocortex. Monoaminergic synapses can co-release neuropeptides either by precisely terminating on cortical neurons or, when being “en passant”, can instigate local volume transmission. Especially due to its early damage, malfunction of the ascending monoaminergic system emerges as an early sign and possible trigger of AD. This review summarizes the involvement and cascaded impairment of brainstem monoaminergic neurons in AD and discusses cellular mechanisms that lead to their dysfunction. We highlight the significance and therapeutic challenges of transmitter co-release in ascending activating system, describe the role and changes of local connections and distant afferents of brainstem nuclei in AD, and summon the rapidly increasing diagnostic window during the last few years.
Impact of Procedural Sedation on the Clinical Outcome of Microelectrode Recording Guided Deep Brain Stimulation in Patients with Parkinson’s Disease
Authors: Bos Michael J. , de Korte-de Boer Dianne , Alzate Sanchez Ana Maria , Duits Annelien , Ackermans Linda , Temel Yasin , Absalom Anthony R. , Buhre Wolfgang F. , Roberts Mark J. , Janssen Marcus L. F.
Published: April 2021

Background: Subthalamic nucleus (STN) deep brain stimulation (DBS) has become a routine treatment of advanced Parkinson’s disease (PD). DBS surgery is commonly performed under local anesthesia (LA) to obtain reliable microelectrode recordings. However, procedural sedation and/or analgesia (PSA) is often desirable to improve patient comfort. The impact of PSA in addition to LA on outcome is largely unknown. Therefore, we performed an observational study to assess the effect of PSA compared to LA alone during STN DBS surgery on outcome in PD patients. Methods: Seventy PD patients (22 under LA, 48 under LA + PSA) scheduled for STN DBS implantation were included. Dexmedetomidine, clonidine or remifentanil were used for PSA. The primary outcome was the change in Movement Disorders Society Unified Parkinson’s Disease Rating Score III (MDS-UPDRS III) and levodopa equivalent daily dosage (LEDD) between baseline, one month before surgery, and twelve months postoperatively. Secondary outcome measures were motor function during activities of daily living (MDS-UPDRS II), cognitive alterations and surgical adverse events. Postoperative assessment was conducted in “on” stimulation and “on” medication conditions. Results: At twelve months follow-up, UPDRS III and UPDRS II scores in “on” medication conditions were similar between the LA and PSA groups. The two groups showed a similar LEDD reduction and an equivalent decline in executive function measured by the Stroop Color-Word Test, Trail Making Test-B, and verbal fluency. The incidence of perioperative and postoperative adverse events was similar between groups. Conclusion: This study demonstrates that PSA during STN DBS implantation surgery in PD patients was not associated with differences in motor and non-motor outcome after twelve months compared with LA only.
Effect of Mycophenolate Mofetil Therapy on Recurrence of Hepatocellular Carcinoma after Liver Transplantation: A Population-Based Cohort Study
Authors: Tsai Yung-Fong , Liu Fu-Chao , Chen Chun-Yu , Lin Jr-Rung , Yu Huang-Ping
Published: April 2021

Hepatocellular carcinoma (HCC) recurrence after liver transplantation is associated with immunosuppressants. However, the appropriate immunosuppressant for HCC recipients is still debated. Data for this nationwide population-based cohort study were extracted from the National Health Insurance Research Database of Taiwan. A total of 1250 liver transplant recipients (LTRs) with HCC were included. We analyzed the risk factors for post-transplant HCC recurrences. Cumulative defined daily dose (cDDD) represented the exposure duration and was calculated as the amount of dispensed defined daily dose (DDD) of mycophenolate mofetil (MMF). The dosage effects of MMF on HCC recurrence and liver graft complication rates were investigated. A total of 155 LTRs, having experienced post-transplant HCC recurrence, exhibited low survival probability at 1-, 3-, 5-, and 10-year observations. Our results demonstrated increased HCC recurrence rate after liver transplantation (p = 0.0316) following MMF administration; however, no significant increase was demonstrated following cyclosporine, tacrolimus, or sirolimus administration. Notably, our data demonstrated significantly increased HCC recurrence rate following MMF administration with cDDD > 0.4893 compared with cDDD ≤ 0.4893 or no administration of MMF (p < 0.0001). MMF administration significantly increases the risk of HCC recurrence. Moreover, a MMF-minimizing strategy (cDDD ≤ 0.4893) is recommended for recurrence-free survival.
The Impact of Riser-Induced Slugs on the Downstream Deoiling Efficiency
Authors: Pedersen Simon , Bram Mads Valentin
Published: April 2021

In Oil and gas productions, the severe slug is an undesired flow regime due to the negative impact on the production rate and facility safety. This study examines the severe riser-induced slugs’ influence on a typical separation process, consisting of a 3-phase gravity separator physically linked to a deoiling hydrocyclone. Four inflow scenarios are compared: Uncontrolled, open-loop, feasible, and infeasible closed-loop anti-slug control, respectively. Three PID controllers’ coefficients are kept constant for all the tests: The separator pressure, water level, and hydrocyclone pressure-drop-ratio (PDR) controllers. The simulation results show that the separation efficiency is significantly larger in the closed-loop configuration, probably due to the larger production rates which provide a preferable operation condition for the hydrocyclone. It is concluded that both slug elimination approaches improve the separation efficiency consistency, but that the closed-loop control provides the best overall separation performance.
Epidemiological Trends of Candidemia and the Impact of Adherence to the Candidemia Guideline: Six-Year Single-Center Experience
Authors: Kim Jong Hun , Suh Jin Woong , Kim Min Ja
Published: April 2021

This study aimed to investigate the epidemiology of candidemia and evaluate the impact of adherence to the candidemia guideline defined by the European Confederation of Medical Mycology Quality of Clinical Candidemia Management (EQUAL) Candida score. Adult candidemia patients ≥ 19 years diagnosed at a tertiary care hospital in the Republic of Korea from 2013 to 2018 were enrolled (period 1 2013–2015, period 2 2016–2018). There was a total of 223 patients. The annual incidence of candidemia increased from 0.43 to 1.33 cases per 1000 admissions between 2013 and 2018, p < 0.001. A significant increase of fluconazole-resistant C. parapsilosis candidemia was noted in period 2 (35.3%) when compared to period 1 (0.0%), p = 0.020. The 30-day mortality rate was not different between period 1 and 2 (43.5% vs. 48.1%, p = 0.527). Multivariate analysis revealed that a Charlson comorbidity index score ≥ 4, neutropenia, duration of hospital stay ≥ 21 days before candidemia diagnosis, septic shock, mycological failure, and EQUAL Candida score < 15 were significantly associated with 30-day mortality. An increase in the incidence of candidemia and fluconazole resistance in the non-albicans Candida species over time was observed. Disease severity, comorbidities, and lower adherence to the candidemia guideline were associated with mortality.
Isolation and Molecular Characterization of the Romaine Lettuce Phylloplane Mycobiome
Authors: Haelewaters Danny , Urbina Hector , Brown Samuel , Newerth-Henson Shannon , Aime M. Catherine
Published: April 2021

Romaine lettuce (Lactuca sativa) is an important staple of American agriculture. Unlike many vegetables, romaine lettuce is typically consumed raw. Phylloplane microbes occur naturally on plant leaves; consumption of uncooked leaves includes consumption of phylloplane microbes. Despite this fact, the microbes that naturally occur on produce such as romaine lettuce are for the most part uncharacterized. In this study, we conducted culture-based studies of the fungal romaine lettuce phylloplane community from organic and conventionally grown samples. In addition to an enumeration of all such microbes, we define and provide a discussion of the genera that form the “core” romaine lettuce mycobiome, which represent 85.5% of all obtained isolates: Alternaria, Aureobasidium, Cladosporium, Filobasidium, Naganishia, Papiliotrema, Rhodotorula, Sampaiozyma, Sporobolomyces, Symmetrospora and Vishniacozyma. We highlight the need for additional mycological expertise in that 23% of species in these core genera appear to be new to science and resolve some taxonomic issues we encountered during our work with new combinations for Aureobasidiumbupleuri and Curvibasidium nothofagi. Finally, our work lays the ground for future studies that seek to understand the effect these communities may have on preventing or facilitating establishment of exogenous microbes, such as food spoilage microbes and plant or human pathogens.
Sea-Buckthorn Seed Oil Induces Proliferation of both Normal and Dysplastic Keratinocytes in Basal Conditions and under UVA Irradiation
Authors: Dudau Maria , Vilceanu Alexandra Catalina , Codrici Elena , Mihai Simona , Popescu Ionela Daniela , Albulescu Lucian , Tarcomnicu Isabela , Moise Georgeta , Ceafalan Laura Cristina , Hinescu Mihail E. , Enciu Ana-Maria , Tanase Cristiana
Published: April 2021

Past decades demonstrate an increasing interest in herbal remedies in the public eye, with as many as 80% of people worldwide using these remedies as healthcare products, including those for skin health. Sea buckthorn and its derived products (oil; alcoholic extracts), rich in flavonoids and essential fatty acids, are among these healthcare products. Specifically, sea buckthorn and its derivatives are reported to have antioxidant and antitumor activity in dysplastic skin cells. On the other hand, evidence suggests that the alteration of lipid metabolism is related to increased malignant behavior. Given the paradoxical involvement of lipids in health and disease, we investigated how sea-buckthorn seed oil, rich in long-chain fatty acids, modifies the proliferation of normal and dysplastic skin cells in basal conditions, as well as under ultraviolet A (UVA) radiation. Using real-time analysis of normal and dysplastic human keratinocytes, we showed that sea-buckthorn seed oil stimulated the proliferation of dysplastic cells, while it also impaired the ability of both normal and dysplastic cells to migrate over a denuded area. Furthermore, UVA exposure increased the expression of CD36/SR-B2, a long-chain fatty acid translocator that is related to the metastatic behavior of tumor cells.
MAES_GR: A Web-Based, Spatially Enabled Field Survey Platform for the MAES Implementation in Greece
Authors: Kokkoris Ioannis , Kokkinos Vasileios , Michos Evangelos , Kalogeropoulos Rafail , Charalambides Marios , Kounelis Agisilaos , Iliadou Eleni , Damianidis Christos , Mallinis Giorgos , Bouras Christos , Dimopoulos Panayotis
Published: April 2021

This study presents a standardized approach to collecting, registering, and reporting field-survey data for baseline MAES (Mapping and Assessment of Ecosystems and their Services) information in Greece. This is accomplished through a web-based platform (MAES_GR) exclusively developed under the relevant, nation-wide LIFE-IP 4 NATURA project. Based on the European Commission’s guidance for ecosystem condition (EC) and ecosystem services (ES) MAES studies, we conceptualized and structured an online platform to support EC and ES assessments, integrating all relevant fields of information needed for registering EC and ES parameters. A novel algorithm calculating EC was also developed and it is available as an integral part of the platform. The use of the MAES_GR platform was evaluated during nationwide field surveys efforts, increasing time efficiency and reducing costs. Field recording of EC and ES pinpoint spatial priorities for ecosystem restoration, conservation and sustainable development. This work highlights that MAES implementation can be favored by the use of technology tools such as mobile survey platforms, developed according to scientific needs and policy guidelines. Such tools, apart from the data inventory phase, can be used for data analysis, synthesis and extraction, providing timely, standardized information suitable for reporting at the local, regional, national and European Union scale.
Fit-For-Purpose Applications in Colombia: Defining Land Boundary Conflicts between Indigenous Sikuani and Neighbouring Settler Farmers
Authors: Becerra Laura , Molendijk Mathilde , Porras Nicolas , Spijkers Piet , Reydon Bastiaan , Morales Javier
Published: April 2021

One of the most difficult types of land-related conflict is that between Indigenous peoples and third parties, such as settler farmers or companies looking for new opportunities who are encroaching on Indigenous communal lands. Nearly 30% of Colombia’s territory is legally owned by Indigenous peoples. This article focuses on boundary conflicts between Indigenous peoples and neighbouring settler farmers in the Cumaribo municipality in Colombia. Boundary conflicts here raise fierce tensions: discrimination of the others and perceived unlawful occupation of land. At the request of Colombia’s rural cadastre (Instituto Geográfico Agustín Codazzi (IGAC)), the Dutch cadastre (Kadaster) applied the fit-for-purpose (FFP) land administration approach in three Indigenous Sikuani reserves in Cumaribo to analyse how participatory mapping can provide a trustworthy basis for conflict resolution. The participatory FFP approach was used to map land conflicts between the reserves and the neighbouring settler farmers and to discuss possible solutions of overlapping claims with all parties involved. Both Indigenous leaders and neighbouring settler farmers measured their perceived claims in the field, after a thorough socialisation process and a social cartography session. In a public inspection, field measurements were shown, with the presence of the cadastral authority IGAC. Showing and discussing the results with all stakeholders helped to clarify the conflicts, to reduce the conflict to specific, relatively small, geographical areas, and to define concrete steps towards solutions.
Simulating Agroforestry Adoption in Rural Indonesia: The Potential of Trees on Farms for Livelihoods and Environment
Authors: Nöldeke Beatrice , Winter Etti , Laumonier Yves , Simamora Trifosa
Published: April 2021

In recent years, agroforestry has gained increasing attention as an option to simultaneously alleviate poverty, provide ecological benefits, and mitigate climate change. The present study simulates small-scale farmers’ agroforestry adoption decisions to investigate the consequences for livelihoods and the environment over time. To explore the interdependencies between agroforestry adoption, livelihoods, and the environment, an agent-based model adjusted to a case study area in rural Indonesia was implemented. Thereby, the model compares different scenarios, including a climate change scenario. The agroforestry system under investigation consists of an illipe (Shorea stenoptera) rubber (Hevea brasiliensis) mix, which are both locally valued tree species. The simulations reveal that farmers who adopt agroforestry diversify their livelihood portfolio while increasing income. Additionally, the model predicts environmental benefits: enhanced biodiversity and higher carbon sequestration in the landscape. The benefits of agroforestry for livelihoods and nature gain particular importance in the climate change scenario. The results therefore provide policy-makers and practitioners with insights into the dynamic economic and environmental advantages of promoting agroforestry.
Stand Structure, Biomass and Carbon Storage in Gmelina arborea Plantation at Agricultural Landscape in Foothills of Eastern Himalayas
Authors: Tamang Mendup , Chettri Roman , Vineeta  , Shukla Gopal , Bhat Jahangeer A. , Kumar Amit , Kumar Munesh , Suryawanshi Arpit , Cabral-Pinto Marina , Chakravarty Sumit
Published: April 2021

In the modern era, Gmelina arborea plantations are a hotspot of future research because of their high carbon sequestration potential. The present work was conducted during 2018 to 2020 on a young unmanaged Gmelina farm to understand the ecosystem’s carbon and its dynamics. The study area was categorized into three age classes: ≤5, 5–10, and 10–15 years. In a plantation, Gmelina trees (10%) were randomly selected while other trees (90%) were also taken into the consideration for ecosystem carbon. A stratified random nested quadrate sampling method was adopted for analyzing other vegetation forms under study. Overall, 51 individual species in the studied Gmelina farm were found which includes 23 tree species, 7 shrub species, 16 herbs, 2 climbers, and 3 species of ferns. The estimated quantitative vegetation parameters and diversity indices indicate that the plant assemblages were heterogeneous with native diverse species evenly distributed with fairly higher densities, frequencies, and abundance. Herbs were the most important species followed by shrubs and trees. Consequently, with the increasing age of plantation, the richness of plant species increased. Soil properties were significantly influenced by the age of the plantation but exhibited no discreet trend. Total biomass density and total carbon density increased with increasing plantation age while no drastic variation was found in available soil organic carbon (SOC) because of insignificant variability in litter production. Total carbon, available SOC (up to 60 cm depth) and ecosystem carbon in the three age class plantations fell in the ranges of 54.51–59.91, 48.18–55.73, and 104.81–110.77 Mg ha−1, respectively. The carbon sequestration potential of Gmelina arborea is higher compared to other reported species and highly supportive of converting unutilized agricultural landscapes to reduce the atmospheric carbon dioxide in future.
Utilizing Edible Agar as a Carrier for Dual Functional Doxorubicin-Fe3O4 Nanotherapy Drugs
Authors: Wang Yu-Jyuan , Lin Pei-Ying , Hsieh Shu-Ling , Kirankumar Rajendranath , Lin Hsin-Yi , Li Jia-Huei , Chen Ya-Ting , Wu Hao-Ming , Hsieh Shuchen
Published: April 2021

The purpose of this study was to use agar as a multifunctional encapsulating material to allow drug and ferromagnetism to be jointly delivered in one nanoparticle. We successfully encapsulated both Fe3O4 and doxorubicin (DOX) with agar as the drug carrier to obtain DOX-Fe3O4@agar. The iron oxide nanoparticles encapsulated in the carrier maintained good saturation of magnetization (41.9 emu/g) and had superparamagnetism. The heating capacity test showed that the specific absorption rate (SAR) value was 18.9 ± 0.5 W/g, indicating that the ferromagnetic nanoparticles encapsulated in the gel still maintained good heating capacity. Moreover, the magnetocaloric temperature could reach 43 °C in a short period of five minutes. In addition, DOX-Fe3O4@agar reached a maximum release rate of 85% ± 3% in 56 min under a neutral pH 7.0 to simulate the intestinal environment. We found using fluorescent microscopy that DOX entered HT-29 human colon cancer cells and reduced cell viability by 66%. When hyperthermia was induced with an auxiliary external magnetic field, cancer cells could be further killed, with a viability of only 15.4%. These results show that agar is an efficient multiple-drug carrier, and allows controlled drug release. Thus, this synergic treatment has potential application value for biopharmaceutical carrier materials.
Laboratory Investigation and Numerical Modelling of Concrete Reinforced with Recycled Steel Fibers
Authors: Pająk Małgorzata , Krystek Małgorzata , Zakrzewski Mateusz , Domski Jacek
Published: April 2021

In the last decades, fiber reinforced concrete have emerged as the possible key to revolutionize civil engineering. Among different types of fibers employed in concrete technology to date, the application of recycled steel fibers produced from end-of-life car tires appears to be a viable approach towards environmentally friendly construction. In this study, we demonstrate the laboratory research and numerical analysis of concrete reinforced with waste steel fibers recovered during the recycling process of end-of-life car tires. Concrete mixes with the following fiber contents: 0.5%, 0.75%, 1.0%, 1.25%, and 1.5% per volume were prepared and then tested in three-point bending conditions. The laboratory investigation revealed highly boosted properties of concrete under flexure. We further performed the finite element method (FEM) analysis of 2D models using Atena software in order to develop a material model allowing the numerical modelling of recycled steel fibers reinforced concrete (RSFRC) behavior. The parameters of RSFRC material model have been modified using the inverse analysis until matching the experimental performance of the material. The results, being in good agreement with the laboratory investigation, have indicated a high potential of RSFRC for real scale construction applications.
Utilizing Iron Ore Tailing as Cementitious Material for Eco-Friendly Design of Ultra-High Performance Concrete (UHPC)
Authors: Ling Gang , Shui Zhonghe , Gao Xu , Sun Tao , Yu Rui , Li Xiaosheng
Published: April 2021

In this research, iron ore tailing (IOT) is utilized as the cementitious material to develop an eco-friendly ultra-high performance concrete (UHPC). The UHPC mix is obtained according to the modified Andreasen and Andersen (MAA) packing model, and the applied dosage of IOT is 10%, 20%, and 30% (by weight), respectively. The calculated packing density of different mixtures is consistent with each other. Afterwards, the fresh and hardened performance of UHPC mixtures with IOT are evaluated. The results demonstrate that the workability of designed UHPC mixtures is increased with the incorporation of IOT. The heat flow at an early age of designed UHPC with IOT is attenuated, the compressive strength and auto shrinkage at an early age are consequently reduced. The addition of IOT promotes the development of long-term compressive strength and optimization of the pore structure, thus the durability of designed UHPC is still guaranteed. In addition, the ecological estimate results show that the utilization of IOT for the UHPC design can reduce the carbon emission significantly.
Improved Hydrogenation Kinetics of TiMn1.52 Alloy Coated with Palladium through Electroless Deposition
Authors: Somo Thabang R. , Davids Moegamat W. , Lototskyy Mykhaylo V. , Hato Mpitloane J. , Modibane Kwena D.
Published: April 2021

The deterioration of hydrogen charging performances resulting from the surface chemical action of electrophilic gases such as CO2 is one of the prevailing drawbacks of TiMn1.52 materials. In this study, we report the effect of autocatalytic Pd deposition on the morphology, structure, and hydrogenation kinetics of TiMn1.52 alloy. Both the uncoated and Pd-coated materials were characterized using scanning electron microscopy/energy dispersive spectroscopy (SEM/EDS) and X-ray diffraction (XRD). XRD analyses indicated that TiMn1.52 alloy contains C14-type Laves phase without any second phase, while the SEM images, together with a particle size distribution histogram, showed a smooth non-porous surface with irregular-shaped particles ranging in size from 1 to 8 µm. The XRD pattern of Pd-coated alloy revealed that C14-type Laves phase was still maintained upon Pd deposition. This was further supported by calculated crystallite size of 29 nm for both materials. Furthermore, a Sieverts-type apparatus was used to study the kinetics of the alloys after pre-exposure to air and upon vacuum heating at 300 °C. The Pd-coated AB2 alloy exhibited good coating quality as confirmed by EDS with enhanced hydrogen absorption kinetics, even without activation. This is attributed to improved surface tolerance and a hydrogen spillover mechanism, facilitated by Pd nanoparticles. Vacuum heating at 300 °C resulted in removal of surface barriers and showed improved hydrogen absorption performances for both coated and uncoated alloys.
Editorial for the Special Issue on ‘Metal Oxide Thin Film: Synthesis, Characterization, and Application’
Authors: Rauwel Erwan , Rauwel Protima
Published: April 2021

The last two decades have witnessed the development of new technologies for thin-film deposition and coating [...]
Ethical Reasoning in Mathematics: New Directions for Didactics in U.S. Mathematics Education
Authors: Register Jordan , Stephan Michelle , Pugalee David
Published: April 2021

In this article we analyze the current state of the didactics of mathematics in the U.S. as it relates to research on equity. The sociopolitical turn in U.S. mathematics education resulted in a push for critical mathematics pedagogies (CMPs) in which predominantly marginalized populations of students explore social injustices through mathematics. We argue that mathematics education for equity must access broader populations of students and develop students’ ethical reasoning skills to build an ethical and equitable future. To address didactical limitations in U.S. mathematics education, we introduce the Ethical Reasoning in Mathematics Framework (ERiM) as a tool to analyze high school students’ ethical reasoning in real-world mathematics tasks. Using data from the initial phase of a Design Research project with students of privilege, we show how the ERiM Framework can be used to design lessons that elicit students’ critical math consciousness (CMC). We end the article with design implications and recommendations for future research on the didactics of equitable mathematics.
Accelerated Solvent Extraction and Pulsed Electric Fields for Valorization of Rainbow Trout (Oncorhynchus mykiss) and Sole (Dover sole) By-Products: Protein Content, Molecular Weight Distribution and Antioxidant Potential of the Extracts
Authors: Wang Min , Zhou Jianjun , Collado Maria Carmen , Barba Francisco J.
Published: April 2021

Fishery by-products are rich in biologically active substances and the use of green and efficient extraction methods to recover these high-added-value compounds is of particular importance. In this study, head, skin and viscera of rainbow trout and sole were used as the target matrices and accelerated solvent extraction (ASE) (45–55 °C, 15 min, pH 5.2–6.8, 103.4 bars) and pulsed electric fields (PEF) (1–3 kV/cm, 123–300 kJ/kg, 15–24 h) were applied as extraction technologies. The results showed that ASE and PEF significantly increased the protein extract efficiency of the fish by-products (p < 0.05) by up to 80%. SDS-PAGE results showed that ASE and PEF treatments changed the molecular size distribution of the protein in the extracts, which was specifically expressed as the change in the area or number of bands between 5 and 250 kDa. The antioxidant capacity of the extracts was evaluated by oxygen radical absorbance capacity (ORAC) and total antioxidant capacity (ABTS) assays. The results showed that both ASE and PEF treatments significantly increased the antioxidant capacity of rainbow trout and sole skin and head extracts (p < 0.05). ASE and PEF extraction processes can be used as new technologies to extract high-added-value compounds from fish by-products.
Analysis of Valorization Process of Aluminum Breakage Scraps to Obtain Green Hydrogen
Authors: Salueña Berna Xavier , Marín-Genescà Marc , Dagà-Monmany José María
Published: April 2021

In this article, it is proposed to use aluminum breakage scraps to obtain green hydrogen through the aluminum–water reaction with caustic soda as a catalyst with experimental research. From this exothermic reaction, both hydrogen and the heat generated can be used. Due to the low price of aluminum chips, this allows us to produce green hydrogen below the current price that is obtained using renewable energy sources and electrolyzers. We have also developed a process that is sustainable since it is obtained as alumina and caustic soda waste that can be reused. This alumina obtained, once filtered, has high purity which allows us to produce high-quality primary aluminum without the need to use bauxite and the production of red sludge is also reduced. A comparative study-analysis was carried out between two of the forms in which the most common aluminum is presented in industry to analyze which one performs better by studying key factors such as the hydrogen produced, and the waste generated during the process. Finally, the mathematical model has been defined to be able to control the flow based on different key parameters such as temperature, molarity, and geometry. Undoubtedly, the study that we present represents a milestone for the recovery of metallic aluminum waste and may be of great interest to industries that use aluminum in their processes, such as recuperators, as well as the vehicle and aerospace industries.
The Effects of HLAW Parameters for One Side T-Joints in 15 mm Thickness Naval Steel
Authors: Churiaque Cristina , Sánchez-Amaya Jose Maria , Porrúa-Lara Manuel , Gumenyuk Andrey , Rethmeier Michael
Published: April 2021

The present contribution is the first research reporting full penetration HLAW joints in 15 mm thick EH36 steel butt T-welds with square grooves on 2F welding position by single-sided welding. The effects of welding parameters were investigated to increase the quality of the joints. Conditions leading to defect-free full penetration welds fulfilling naval regulations includes a laser power of 12.5 kW, a welding speed of 1.6 m/min and the vertical laser offset distance from the flange of 1 mm. Advanced characterization of selected welds included a microstructural identification by optical microscopy, SEM, and XRD, revealing the presence of acicular, polygonal and Widmanstätten ferrite, lath martensite, and some retained austenite at FZ. Hardness and microhardness mapping tests showed values of 155 HV at base metal and 200 to 380 HV at the fusion zone connecting the web to the flange.
Beneficial Metabolic Effect of a Nutraceuticals Combination (Monacolin K, Yeasted Red Rice, Polyphenolic Extract of Annurca Apple and Berberine) on Acquired Hypercholesterolemia: A Prospective Analysis
Authors: D’Assante Roberta , De Luca Mariarosaria , Ferraro Sergio , Ferraro Andrea , Ruvolo Antonio , Natale Francesco , Sotgiu Pietro , Petitto Maurizio , Rizzo Romolo , De Maria Umberto , Liguori Luigi , Gentile Gianluigi , Ragucci Paola , Donadio Vittorio , Valente Valeria , Cittadini Antonio
Published: April 2021

Hypercholesterolemia represents a serious public health problem as it significantly increases the risk of developing cardiovascular diseases. Its treatment with statin is limited by costs, side effects, and drugs interactions. Nutraceuticals appear to have an important metabolic effect on cholesterol reduction as well as on body weight and glycemia. The aim of this study was to evaluate the effect of a nutraceutical combination (Melasterol) in eighty-seven patients with acquired hypercholesterolemia. Clinically relevant parameters were collected at baseline and after three and six months of Melasterol treatment, one tablet per day. The primary endpoint was the change in cholesterol and triglyceride levels. Six months of treatment resulted in a 19.2% decrease in total cholesterol, accompanied by a 19.8% decrease in low-density lipoprotein (LDL) and a 23% reduction in triglycerides (p < 0.001) but not in high-density lipoprotein (HDL) levels (p > 0.05). These results were paralleled by a significative blood glucose (108.3 ± 21.3 vs. 98.4 ± 18.6 mg/dL p < 0.001) and body mass index (BMI) reduction (27.8 ± 4.4 vs. 27.0 ± 4.2 mg/dL, p < 0.001). A subgroup of 12 patients performed flow-mediated dilation, with values increasing by 1.8% (p < 0.05). No significant side effects were reported. Besides its cholesterol-lowering effect, Melasterol was associated with a significant improvement in other relevant metabolic parameters such as BMI and glycemia.
Metabolic Markers Demonstrate the Heterogeneity of Myosteatosis in Community-Dwelling Older Black Men from the Health ABC Study
Authors: Farsijani Samaneh , Marron Megan M. , Miljkovic Iva , Baugh Mary Elizabeth , Kritchevsky Stephen B. , Newman Anne B.
Published: April 2021

Myosteatosis is a complex condition, associated with aging and diverse pathological conditions (e.g., diabetes), that contributes to mobility disability. Improved characterization of myosteatosis is required to develop targeted interventions to maintain muscle health in aging. We first determined the associations between plasma metabolites and intermuscular fat (IMF) in a cross-sectional analysis of 313 older Black men from Health ABC Study. Using partial correlation analysis, 34/350 metabolites were associated with IMF, the majority of which were lipids and organic acids. Next, we used Homeostasis Model Assessment of Insulin Resistance (HOMA-IR), as an indicator of metabolic health to delineate the anthropometric, functional, and metabolic heterogeneity of myosteatosis in a case-control matching analysis. We categorized participants based on their IMF and HOMA-IR levels into: Low-IMF with Low- versus High-HOMA, as well as High-IMF with Low- versus High-HOMA. Among participants with similar levels of IMF, those who were metabolically unhealthy, i.e., with High HOMA-IR, had higher fat and lean mass, muscle strength, and had hyperglycemia, hypertriglyceridemia, hyperinsulinemia, and higher levels of plasma metabolites belonging to diacylglycerols, triacylglycerols, fatty acid and aminoacyl-tRNA biosynthesis pathways versus those with Low HOMA-IR. In summary, HOMA-IR delineates the heterogeneity of myosteatosis by distinguishing metabolically healthy versus unhealthy individuals.
Synthesis and Biological Evaluation of Coumarin-Linked 4-Anilinomethyl-1,2,3-Triazoles as Potent Inhibitors of Carbonic Anhydrases IX and XIII Involved in Tumorigenesis
Authors: Thacker Pavitra S. , Tiwari Prerna L. , Angeli Andrea , Srikanth Danaboina , Swain Baijayantimala , Arifuddin Mohammed , Supuran Claudiu T.
Published: April 2021

A series of coumarin-linked 4-anilinomethyl-1,2,3-triazoles (6a–t) was synthesized via a molecular hybridization approach, through carbon C-6 of the coumarin moiety. The synthesized compounds were evaluated for their inhibition of carbonic anhydrase (CA) isoforms I, II, IX and XIII. CAs IX and XIII were selectively inhibited over the off-target isoforms I and II. The best inhibitory profiles against CA IX were shown by compounds 6a, 6e and 6f (Ki < 50 nM), with compound 6e displaying the best inhibition with a Ki value of 36.3 nM. Compounds 6a, 6b, 6j, 6o and 6q exhibited the best inhibitory profiles against CA XIII (Ki < 100 nM). These compounds can be further explored for the discovery of potent and effective CA IX and CA XIII inhibitors.
Magnetic Driven Two-Finger Micro-Hand with Soft Magnetic End-Effector for Force-Controlled Stable Manipulation in Microscale
Authors: Liu Dan , Liu Xiaoming , Li Pengyun , Tang Xiaoqing , Kojima Masaru , Huang Qiang , Arai Tatsuo
Published: April 2021

In recent years, micromanipulators have provided the ability to interact with micro-objects in industrial and biomedical fields. However, traditional manipulators still encounter challenges in gaining the force feedback at the micro-scale. In this paper, we present a micronewton force-controlled two-finger microhand with a soft magnetic end-effector for stable grasping. In this system, a homemade electromagnet was used as the driving device to execute micro-objects manipulation. There were two soft end-effectors with diameters of 300 μm. One was a fixed end-effector that was only made of hydrogel, and the other one was a magnetic end-effector that contained a uniform mixture of polydimethylsiloxane (PDMS) and paramagnetic particles. The magnetic force on the soft magnetic end-effector was calibrated using an atomic force microscopy (AFM) probe. The performance tests demonstrated that the magnetically driven soft microhand had a grasping range of 0–260 μm, which allowed a clamping force with a resolution of 0.48 μN. The stable grasping capability of the magnetically driven soft microhand was validated by grasping different sized microbeads, transport under different velocities, and assembly of microbeads. The proposed system enables force-controlled manipulation, and we believe it has great potential in biological and industrial micromanipulation.
Regulation of Plant Mineral Nutrition by Signal Molecules
Authors: Kalia Vipin Chandra , Gong Chunjie , Patel Sanjay K. S. , Lee Jung-Kul
Published: April 2021

Microbes operate their metabolic activities at a unicellular level. However, it has been revealed that a few metabolic activities only prove beneficial to microbes if operated at high cell densities. These cell density-dependent activities termed quorum sensing (QS) operate through specific chemical signals. In Gram-negative bacteria, the most widely reported QS signals are acylhomoserine lactones. In contrast, a novel QS-like system has been elucidated, regulating communication between microbes and plants through strigolactones. These systems regulate bioprocesses, which affect the health of plants, animals, and human beings. This mini-review presents recent developments in the QS and QS-like signal molecules in promoting plant health.
Ratiometric Electrochemistry: Improving the Robustness, Reproducibility and Reliability of Biosensors
Authors: Spring Sam A. , Goggins Sean , Frost Christopher G.
Published: April 2021

Electrochemical biosensors are an increasingly attractive option for the development of a novel analyte detection method, especially when integration within a point-of-use device is the overall objective. In this context, accuracy and sensitivity are not compromised when working with opaque samples as the electrical readout signal can be directly read by a device without the need for any signal transduction. However, electrochemical detection can be susceptible to substantial signal drift and increased signal error. This is most apparent when analysing complex mixtures and when using small, single-use, screen-printed electrodes. Over recent years, analytical scientists have taken inspiration from self-referencing ratiometric fluorescence methods to counteract these problems and have begun to develop ratiometric electrochemical protocols to improve sensor accuracy and reliability. This review will provide coverage of key developments in ratiometric electrochemical (bio)sensors, highlighting innovative assay design, and the experiments performed that challenge assay robustness and reliability.
Effects of a Community-Based Behavioral Intervention with a Traditional Atlantic Diet on Cardiometabolic Risk Markers: A Cluster Randomized Controlled Trial (“The GALIAT Study”)
Authors: Calvo-Malvar Mar , Benítez-Estévez Alfonso J. , Sánchez-Castro Juan , Leis Rosaura , Gude Francisco
Published: April 2021

The Atlantic diet, the traditional dietary pattern in northern Portugal and northwest Spain, has been related to metabolic health and low ischemic heart disease mortality. The Galiat Study is a randomized controlled trial aimed to assess the effects of the Atlantic diet on anthropometric variables, metabolic profile, and nutritional habits. The dietary intervention was conducted in 250 families (720 adults and children) and performed at a primary care center. Over six months, families randomized to the intervention group received educational sessions, cooking classes, written supporting material, and foods that form part of the Atlantic diet, whereas those randomized to the control group followed their habitual lifestyle. 213 families (92.4%) completed the trial. Adults in the intervention group lost weight as opposed to controls who gained weight (adjusted mean difference −1.1 kg, p < 0.001) and total serum cholesterol (adjusted mean difference −5.2 mg/dL, p = 0.004). Significant differences in favor of the intervention were found in other anthropometric variables and low-density lipoprotein cholesterol, but changes in triglycerides, high-density lipoprotein cholesterol, inflammation markers, blood pressure, and glucose metabolism were not observed. A family-based nutritional intervention based on the Atlantic diet showed beneficial effects on adiposity and the lipid profile.
Maternal Obesity and Risk of Low Birth Weight, Fetal Growth Restriction, and Macrosomia: Multiple Analyses
Authors: Lewandowska Małgorzata
Published: April 2021

The associations between maternal pre-pregnancy obesity and low birth weight (LBW, <2500 g) remain inconclusive. Therefore, birth weight in a Polish prospective cohort of 912 mothers was investigated depending on the pre-pregnancy body mass index (BMI). The whole cohort and the subgroup of gestational weight gain (GWG) in the range of the Institute of Medicine (IOM) recommendations, as well as ‘healthy’ women (who did not develop diabetes or hypertension in this pregnancy) were investigated. Adjusted odds ratios (AOR) of the newborn outcomes (with 95% confidence intervals, CI) for obesity (BMI ≥ 30 kg/m2) vs. normal BMI (18.5−24.9 kg/m2) were calculated using multiple logistic regression. Risk profiles (in the Lowess method) were presented for BMI values (kg/m2) and threshold BMI values were calculated. (1) In the cohort, LBW affected 6.6% of pregnancies, fetal growth restriction (FGR) 2.3%, and macrosomia 10.6%. (2) The adjusted risk of macrosomia was more than three-fold higher for obesity compared to normal BMI in the whole cohort (AOR = 3.21 (1.69−6.1), p < 0.001) and the result was maintained in the subgroups. A 17-fold higher adjusted LBW risk for obesity was found (AOR = 17.42 (1.5−202.6), p = 0.022), but only in the normal GWG subgroup. The FGR risk profile was U-shaped: in the entire cohort, the risk was more than three times higher for obesity (AOR = 3.12 (1.02−9.54), p = 0.045) and underweight (AOR = 3.84 (1.13−13.0), p = 0.031). (3) The risk profiles showed that the highest BMI values were found to be associated with a higher risk of these three newborn outcomes and the threshold BMI was 23.7 kg/m2 for macrosomia, 26.2 kg/m2 for LBW, and 31.8 kg/m2 for FGR. These results confirm the multidirectional effects of obesity on fetal growth (low birth weight, fetal growth restriction, and macrosomia). The results for LBW were heavily masked by the effects of abnormal gestational weight gain.