bluuebunny/crossref_metadata_2025 huggingface dataset:
Shear Behavior of Steel-Fiber-Reinforced Recycled Aggregate Concrete Deep Beams
Authors: Kachouh Nancy , El-Maaddawy Tamer , El-Hassan Hilal , El-Ariss Bilal
Published: September 2021

Results of an experimental investigation aimed at studying the effect of steel fibers on the shear behavior of concrete deep beams made with a 100% recycled concrete aggregate (RCA) are presented in this paper. The study comprised testing of seven concrete deep beam specimens with a shear span-to-depth ratio (a/h) of 1.6. Two beams were made of natural aggregates (NAs) without steel fibers, two beams were made of a 100% RCA without steel fibers, and three beams were made of RCA-based concrete with steel fibers at volume fractions (vf) of 1, 2, and 3%. Two of the beams without steel fibers included a minimum shear reinforcement. Test results showed that the beam with a 100% RCA without steel fibers exhibited a lower post-cracking stiffness, reduced shear cracking load, and lower shear capacity than those of the NA-based control beam. The detrimental effect of the RCA on the shear response was less pronounced in the presence of the minimum shear reinforcement. The addition of steel fibers significantly improved the shear response of the RCA-based beams. The post-cracking stiffness of the RCA-based concrete beams with steel fibers coincided with that of a similar beam without fibers containing the minimum shear reinforcement. The use of steel fibers in RCA beams at vf of 1 and 2% restored 80 and 90% of the shear capacity, respectively, of a similar beam with the minimum shear reinforcement. The response of the RCA specimen with vf of 3% outperformed that of the NA-based control beam with the minimum shear reinforcement, indicating that steel fibers can be used in RCA deep beams as a substitution to the minimum shear reinforcement. The shear capacities obtained from the tests were compared with predictions of published analytical models.
Damage Assessment of Reinforced Concrete Elements Due to Corrosion Effect Using Dynamic Parameters: A Review
Authors: Duvnjak Ivan , Klepo Ivan , Serdar Marijana , Damjanović Domagoj
Published: September 2021

Corrosion of reinforcement is one of the main problems related to the durability of reinforced concrete structures. This can cause cracks and a separation of the protective layer, as well as reducing strength and structural stiffness, which can result in numerous human casualties. Visual inspection is a standard method of assessing the condition of reinforced concrete structures whose limitations, such as time, interpretability, accessibility, etc., may affect its effectiveness. Therefore, damage determination methods based on dynamic parameters are becoming more and more prominent in the assessment of damage to reinforced concrete structures. The aim of this paper is to review the literature regarding the determination of corrosion of reinforcement by methods based on dynamic parameters, and to identify future research to develop a method that would detect corrosion problems in time through a continuous system of structural health monitoring.
Adoptive NK Cell Therapy: A Promising Treatment Prospect for Metastatic Melanoma
Authors: van Vliet Amanda A. , Georgoudaki Anna-Maria , Raimo Monica , de Gruijl Tanja D. , Spanholtz Jan
Published: September 2021

Adoptive cell therapy (ACT) represents a promising alternative approach for patients with treatment-resistant metastatic melanoma. Lately, tumor infiltrating lymphocyte (TIL) therapy and chimeric antigen receptor (CAR)-T cell therapy have shown improved clinical outcome, compared to conventional chemotherapy or immunotherapy. Nevertheless, they are limited by immune escape of the tumor, cytokine release syndrome, and manufacturing challenges of autologous therapies. Conversely, the clinical use of Natural Killer (NK) cells has demonstrated a favorable clinical safety profile with minimal toxicities, providing an encouraging treatment alternative. Unlike T cells, NK cells are activated, amongst other mechanisms, by the downregulation of HLA class I molecules, thereby overcoming the hurdle of tumor immune escape. However, impairment of NK cell function has been observed in melanoma patients, resulting in deteriorated natural defense. To overcome this limitation, “activated” autologous or allogeneic NK cells have been infused into melanoma patients in early clinical trials, showing encouraging clinical benefit. Furthermore, as several NK cell-based therapeutics are being developed for different cancers, an emerging variety of approaches to increase migration and infiltration of adoptively transferred NK cells towards solid tumors is under preclinical investigation. These developments point to adoptive NK cell therapy as a highly promising treatment for metastatic melanoma in the future.
Neoadjuvant Treatment Strategies in Resectable Pancreatic Cancer
Authors: Lambert Aurélien , Schwarz Lilian , Ducreux Michel , Conroy Thierry
Published: September 2021

Complete surgical resection is the cornerstone of curative therapy for resectable pancreatic adenocarcinoma. Upfront surgery is the gold standard, but it is rarely curative. Neoadjuvant treatment is a logical option, as it may overcome some of the limitations of adjuvant therapy and has already shown some encouraging results. The main concern regarding neoadjuvant therapy is the risk of disease progression during chemotherapy, meaning the opportunity to undergo the intended curative surgery is missed. We reviewed all recent literature in the following areas: major surveys, retrospective studies, meta-analyses, and randomized trials. We then selected the ongoing trials that we believe are of interest in this field and report here the results of a comprehensive review of the literature. Meta-analyses and randomized trials suggest that neoadjuvant treatment has a positive effect. However, no study to date can be considered practice changing. We considered design, endpoints, inclusion criteria and results of available randomized trials. Neoadjuvant treatment appears to be at least a feasible strategy for patients with resectable pancreatic cancer.
Characteristics of a Novel ATP2B3 K416_F418delinsN Mutation in a Classical Aldosterone-Producing Adenoma
Authors: Liao Hung-Wei , Peng Kang-Yung , Wu Vin-Cent , Lin Yen-Hung , Lin Shuei-Liong , Lin Wei-Chou , Chueh Jeff S. ,   on behalf of (TAIPAI) Study Group
Published: September 2021

In patients with primary aldosteronism (PA), the prevalence of ATP2B3 mutation is rare. The aim of this study is to report a novel ATP2B3 mutation in a PA patient. Based on our tissue bank of aldosterone-producing adenomas (APA), we identified a novel somatic ATP2B3 K416_F418delinsN mutation. The affected individual was a 53 year-old man with a 4 year history of hypertension. Computed tomography (CT) showed bilateral adrenal masses of 1.6 (left) and 0.5 cm (right) in size. An adrenal venous sampling (AVS) showed a lateralization index (LI) of 2.2 and a contralateral suppression index (CLS) of 0.12; indicating left functional predominance. After a left unilateral adrenalectomy, he achieved partial biochemical and hypertension–remission. This classical adenoma harbored a novel ATP2B3 K416_F418delinsN somatic mutation, which is a deletion from nucleotides 1248 to 1253. The translated amino acid sequence from 416 to 418, reading as lysine-phenylalanine-phenylalanine, was deleted; however, an asparagine was inserted due to merging of residual nucleotide sequences. The CYP11B2 immunohistochemistry staining demonstrated strong immunoreactivity in this classical adenoma. The ATP2B3 K416_F418delinsN mutation is a functional mutation in APA, since HAC15 cells, a human adrenal cell line, transfected with the mutant gene showed increased CYP11B2 expression and aldosterone production.
HPV Status as Prognostic Biomarker in Head and Neck Cancer—Which Method Fits the Best for Outcome Prediction?
Authors: Kühn Jan Philipp , Schmid Wendelin , Körner Sandrina , Bochen Florian , Wemmert Silke , Rimbach Hugo , Smola Sigrun , Radosa Julia Caroline , Wagner Mathias , Morris Luc G.T. , Bozzato Victoria , Bozzato Alessandro , Schick Bernhard , Linxweiler Maximilian
Published: September 2021

The incidence of human papillomavirus (HPV)-related head and neck cancer (HNSCC) is rising globally, presenting challenges for optimized clinical management. To date, it remains unclear which biomarker best reflects HPV-driven carcinogenesis, a process that is associated with better therapeutic response and outcome compared to tobacco/alcohol-induced cancers. Six potential HPV surrogate biomarkers were analyzed using FFPE tissue samples from 153 HNSCC patients (n = 78 oropharyngeal cancer (OPSCC), n = 35 laryngeal cancer, n = 23 hypopharyngeal cancer, n = 17 oral cavity cancer): p16, CyclinD1, pRb, dual immunohistochemical staining of p16 and Ki67, HPV-DNA-PCR, and HPV-DNA-in situ hybridization (ISH). Biomarkers were analyzed for correlation with one another, tumor subsite, and patient survival. P16-IHC alone showed the best performance for discriminating between good (high expression) vs poor outcome (low expression; p = 0.0030) in OPSCC patients. Additionally, HPV-DNA-ISH (p = 0.0039), HPV-DNA-PCR (p = 0.0113), and p16-Ki67 dual stain (p = 0.0047) were significantly associated with prognosis in uni- and multivariable analysis for oropharyngeal cancer. In the non-OPSCC group, however, none of the aforementioned surrogate markers was prognostic. Taken together, P16-IHC as a single biomarker displays the best diagnostic accuracy for prognosis stratification in OPSCC patients with a direct detection of HPV-DNA by PCR or ISH as well as p16-Ki67 dual stain as potential alternatives.
Risk Factors for Radiation Necrosis in Patients Undergoing Cranial Stereotactic Radiosurgery
Authors: Kerschbaumer Johannes , Demetz Matthias , Krigers Aleksandrs , Nevinny-Stickel Meinhard , Thomé Claudius , Freyschlag Christian F.
Published: September 2021

Purpose: single-staged stereotactic radiosurgery (SRS) is an established part of the multimodal treatment in neuro-oncology. Radiation necrosis after high-dose irradiation is a known complication, but there is a lack of evidence about the risk factors. The aim of this study was to evaluate possible risk factors for radiation necrosis in patients undergoing radiosurgery. Methods: patients treated with radiosurgery between January 2004 and November 2020 were retrospectively analyzed. The clinical data, imaging and medication were gathered from electronic patient records. The largest diameter of the tumors was measured using MRI scans in T1 weighted imaging with gadolinium and the edema in T2 weighted sequences. The diagnosis of a radiation necrosis was established analyzing imaging criteria combined with clinical course or pathologically confirmed by subsequent surgical intervention. Patients developing radiation necrosis detected after SRS were compared to patients without evidence of an overshooting irradiation reaction. Results: 388 patients were included retrospectively, 61 (15.7%) of whom developed a radiation necrosis. Median follow-up was 24 (6–62) months with a radiation necrosis after 8 (6–12) months. The most frequent tumors were metastases in 47.2% of the cases, followed by acoustic neuromas in 32.2% and meningiomas in 13.4%. Seventy-three (18.9%) patients already underwent one or more previous radiosurgical procedures for different lesions. The mean largest diameter of the tumors amounted to 16.3 mm (±6.1 mm). The median—80%—isodose administered was 16 (14–25) Gy. Of the radiation necroses, 25 (43.1%) required treatment, in 23 (39.7%) thereof, medical treatment was applied and in 2 (3.4%) cases, debulking surgery was performed. In this study, significantly more radiation necroses arose in patients with higher doses (HR 1.3 [CI 1.2; 1.5], p < 0.001) leading to a risk increment of over 180% between a radiation isodose of 14 and 20 Gy. The maximum diameter was a second significant risk factor (p = 0.028) with an HR of 1065 for every 1 mm increase in multivariate analysis. Conclusion: large diameter and high doses were reliable independent risk factors leading to more frequent radiation necroses, regardless of tumor type in patients undergoing radiosurgery. Alternative therapeutic procedures may be considered in lesions with large volume and an expected high radiation doses due to the increased risk of developing radiation necrosis.
The Landscape of Transmembrane Protein Family Members in Head and Neck Cancers: Their Biological Role and Diagnostic Utility
Authors: Koteluk Oliwia , Bielicka Antonina , Lemańska Żaneta , Jóźwiak Kacper , Klawiter Weronika , Mackiewicz Andrzej , Kazimierczak Urszula , Kolenda Tomasz
Published: September 2021

Background: Transmembrane proteins (TMEM) constitute a large family of proteins spanning the entirety of the lipid bilayer. However, there is still a lack of knowledge about their function or mechanism of action. In this study, we analyzed the expression of selected TMEM genes in patients with head and neck squamous cell carcinoma (HNSCC) to learn their role in tumor formation and metastasis. Materials and Methods: Using TCGA data, we analyzed the expression levels of different TMEMs in both normal and tumor samples and compared those two groups depending on clinical-pathological parameters. We selected four TMEMs whose expression was highly correlated with patient survival status and subjected them to further analysis. The pathway analysis using REACTOME and the gene set enrichment analysis (GSEA) were performed to evaluate the association of those TMEMs with genes involved in hallmarks of cancer as well as in oncogenic and immune-related pathways. In addition, the fractions of different immune cell subpopulations depending on TMEM expression were estimated in analyzed patients. The results for selected TMEMs were validated using GEO data. All analyses were performed using the R package, Statistica, and Graphpad Prism. Results: We demonstrated that 73% of the analyzed TMEMs were dysregulated in HNSCC and depended on tumor localization, smoking, alcohol consumption, or HPV infection. The expression levels of ANO1, TMEM156, TMEM173, and TMEM213 correlated with patient survival. The four TMEMs were also upregulated in HPV-positive patients. The elevated expression of those TMEMs correlated with the enrichment of genes involved in cancer-related processes, including immune response. Specifically, overexpression of TMEM156 and TMEM173 was associated with immune cell mobilization and better survival rates, while the elevated ANO1 expression was linked with metastasis formation and worse survival. Conclusions: In this work, we performed a panel of in silico analyses to discover the role of TMEMs in head and neck squamous cell carcinoma. We found that ANO1, TMEM156, TMEM173, and TMEM213 correlated with clinical status and immune responses in HNSCC patients, pointing them as biomarkers for a better prognosis and treatment. This is the first study describing such the role of TMEMs in HNSCC. Future clinical trials should confirm the potential of those genes as targets for personalized therapy of HNSCC.
Correction: van Bruggen et al. Overcoming the Hurdles of Autologous T-Cell-Based Therapies in B-Cell Non-Hodgkin Lymphoma. Cancers 2020, 12, 3837
Authors: van Bruggen Jaco A. C. , Martens Anne W. J. , Tonino Sanne H. , Kater Arnon P.
Published: September 2021

In the original article [...]
Bevacizumab Combined with Platinum–Taxane Chemotherapy as First-Line Treatment for Advanced Ovarian Cancer: Results of the NOGGO Non-Interventional Study (OTILIA) in 824 Patients
Authors: Sehouli Jalid , Mustea Alexander , Oskay-Özcelik Guelten , Keller Maren , Richter Rolf , Tomé Oliver , Woopen Hannah , Sommer-Joos Ann-Katrin , Grabowski Jacek P. , Armbrust Robert , Wimberger Pauline
Published: September 2021

In the single-arm non-interventional OTILIA study, patients with newly diagnosed International Federation of Gynecology and Obstetrics (FIGO) stage IIIB–IV ovarian cancer received bevacizumab (15 mg/kg every 3 weeks for up to 15 months) and standard carboplatin–paclitaxel. The primary aim was to assess safety and progression-free survival (PFS). Subgroup analyses according to age were prespecified. The analysis population included 824 patients (453 aged <70 years, 371 aged ≥70 years). At data cutoff, the median bevacizumab duration was 13.8 months. Grade ≥3 adverse events (AEs), serious AEs, and AEs leading to bevacizumab discontinuation were more common in older than younger patients, whereas treatment-related AEs were less common. Median PFS was 19.4 months, with no clear difference according to age (20.0 vs. 19.3 months in patients <70 vs. ≥70 years, respectively). One-year OS rates were 92% and 90%, respectively. Mean change from baseline in global health status/quality of life showed a clinically meaningful increase over time. In German routine oncology practice, PFS and safety were similar to reported randomized phase 3 bevacizumab trials in more selected populations. There was no notable reduction in effectiveness and tolerability in patients aged ≥70 years; age alone should not preclude use of bevacizumab-containing therapy. ClinicalTrials.gov: NCT01697488.
Preclinical Evaluation of Podoplanin-Targeted Alpha-Radioimmunotherapy with the Novel Antibody NZ-16 for Malignant Mesothelioma
Authors: Sudo Hitomi , Tsuji Atsushi B. , Sugyo Aya , Kaneko Mika K. , Kato Yukinari , Nagatsu Kotaro , Suzuki Hisashi , Higashi Tatsuya
Published: September 2021

The prognosis of advanced mesothelioma is poor. Podoplanin (PDPN) is highly expressed in most malignant mesothelioma. This study aimed to evaluate the potential alpha-radioimmunotherapy (RIT) with a newly developed anti-PDPN antibody, NZ-16, compared with a previous antibody, NZ-12. Methods: The in vitro properties of radiolabeled antibodies were evaluated by cell binding and competitive inhibition assays using PDPN-expressing H226 mesothelioma cells. The biodistribution of 111In-labeled antibodies was studied in tumor-bearing mice. The absorbed doses were estimated based on biodistribution data. Tumor volumes and body weights of mice treated with 90Y- and 225Ac-labeled NZ-16 were measured for 56 days. Histologic analysis was conducted. Results: The radiolabeled NZ-16 specifically bound to H226 cells with higher affinity than NZ-12. The biodistribution studies showed higher tumor uptake of radiolabeled NZ-16 compared with NZ-12, providing higher absorbed doses to tumors. RIT with 225Ac- and 90Y-labeled NZ-16 had a significantly higher antitumor effect than RIT with 90Y-labeled NZ-12. 225Ac-labeled NZ-16 induced a larger amount of necrotic change and showed a tendency to suppress tumor volumes and prolonged survival than 90Y-labeled NZ-16. There is no obvious adverse effect. Conclusions: Alpha-RIT with the newly developed NZ-16 is a promising therapeutic option for malignant mesothelioma.
A Candidate RNAi Screen Reveals Diverse RNA-Binding Protein Phenotypes in Drosophila Flight Muscle
Authors: Kao Shao-Yen , Nikonova Elena , Chaabane Sabrina , Sabani Albiona , Martitz Alexandra , Wittner Anja , Heemken Jakob , Straub Tobias , Spletter Maria L.
Published: September 2021

The proper regulation of RNA processing is critical for muscle development and the fine-tuning of contractile ability among muscle fiber-types. RNA binding proteins (RBPs) regulate the diverse steps in RNA processing, including alternative splicing, which generates fiber-type specific isoforms of structural proteins that confer contractile sarcomeres with distinct biomechanical properties. Alternative splicing is disrupted in muscle diseases such as myotonic dystrophy and dilated cardiomyopathy and is altered after intense exercise as well as with aging. It is therefore important to understand splicing and RBP function, but currently, only a small fraction of the hundreds of annotated RBPs expressed in muscle have been characterized. Here, we demonstrate the utility of Drosophila as a genetic model system to investigate basic developmental mechanisms of RBP function in myogenesis. We find that RBPs exhibit dynamic temporal and fiber-type specific expression patterns in mRNA-Seq data and display muscle-specific phenotypes. We performed knockdown with 105 RNAi hairpins targeting 35 RBPs and report associated lethality, flight, myofiber and sarcomere defects, including flight muscle phenotypes for Doa, Rm62, mub, mbl, sbr, and clu. Knockdown phenotypes of spliceosome components, as highlighted by phenotypes for A-complex components SF1 and Hrb87F (hnRNPA1), revealed level- and temporal-dependent myofibril defects. We further show that splicing mediated by SF1 and Hrb87F is necessary for Z-disc stability and proper myofibril development, and strong knockdown of either gene results in impaired localization of kettin to the Z-disc. Our results expand the number of RBPs with a described phenotype in muscle and underscore the diversity in myofibril and transcriptomic phenotypes associated with splicing defects. Drosophila is thus a powerful model to gain disease-relevant insight into cellular and molecular phenotypes observed when expression levels of splicing factors, spliceosome components and splicing dynamics are altered.
Absorption and Isomerization of Azobenzene Guest Molecules in Polymeric Nanoporous Crystalline Phases
Authors: Coscia Nicola , Cozzolino Antonietta , Golla Manohar , Rizzo Paola
Published: September 2021

PPO co-crystalline (CC) films including azobenzene guest molecules have been prepared and characterized by WAXD, FTIR and UV-Visible measurements. Isomerization reactions of azobenzene (photo-induced trans to cis and spontaneous cis to trans) included in α and β nanoporous-crystalline (NC) phases leading to CC phases, or simply absorbed in amorphous phase have been studied on thick and thin films. Spectroscopic analysis shows that photo-isomerization of azobenzene occurs without expulsion of azobenzene guest molecules from crystalline phases. Sorption studies of α and β NC films immersed into photo-isomerized azobenzene solution reveal a higher selectivity of the β NC phase toward cis azobenzene isomer than the α NC phase, inducing us to propose the β NC phase as particularly suitable for absorbing spherically bulky guest molecules.
Antioxidant and Anti-Melanogenic Activities of Heat-Treated Licorice (Wongam, Glycyrrhiza glabra × G. uralensis) Extract
Authors: Kang Min Hye , Jang Gwi Yeong , Ji Yun-Jeong , Lee Jeong Hoon , Choi Su Ji , Hyun Tae Kyung , Kim Hyung Don
Published: September 2021

Melanin is a brown or black pigment that protects skin from ultraviolet radiation and reactive oxygen species (ROS). However, overproduction of melanin is associated with lentigines, melasma, freckles and skin cancer. Licorice has shown antioxidant, anti-tumor, anti-platelet, anti-inflammatory and immunomodulatory activities and is used as a natural treatment for skin whitening. We aimed to confirm the potential of Wongam, a new cultivar of licorice developed by the Rural Development Administration (RDA), as a whitening agent in cosmetics. In addition, we verified the effect of heat treatment on the bioactivity of licorice by comparing antioxidant and anti-melanogenic activities of licorice extract before and after heating (130 °C). The heat-treated licorice extract (WH-130) showed higher radical-scavenging activities in the ABTS+ (2,2′-azino-bis-(3-ethylbenzothiazolin-6-sulfonic acid) diammonium salt) and DPPH (2,2-diphenyl-1-picrylhydrazyl) assays. In addition, WH-130 inhibited melanogenesis more effectively due to downregulation of tyrosinase in B16F10 melanoma cells than non-heated licorice extract. Moreover, heat treatment increased total phenolic content. In particular, isoliquiritigenin, an antioxidant and anti-melanogenic compound of licorice, was produced by heat treatment. In conclusion, WH-130, with increased levels of bioactive phenolics such as isoliquiritigenin, has potential for development into a novel skin whitening material with applications in cosmetics.
Hyperlipidemia and Obesity’s Role in Immune Dysregulation Underlying the Severity of COVID-19 Infection
Authors: Khatchadourian Christopher , Sisliyan Christina , Nguyen Kevin , Poladian Nicole , Tian Qi , Tamjidi Faraaz , Luong Bao , Singh Manpreet , Robison Jeremiah , Venketaraman Vishwanath
Published: September 2021

Obesity and hyperlipidemia are known to be risk factors for various pathological disorders, including various forms of infectious respiratory disease, including the current Coronavirus outbreak termed Coronavirus Disease 19 (COVID-19). This review studies the effects of hyperlipidemia and obesity on enhancing the inflammatory response seen in COVID-19 and potential therapeutic pathways related to these processes. In order to better understand the underlying processes of cytokine and chemokine-induced inflammation, we must further investigate the immunomodulatory effects of agents such as Vitamin D and the reduced form of glutathione as adjunctive therapies for COVID-19 disease.
Nightmares in Children with Foetal Alcohol Spectrum Disorders, Autism Spectrum Disorders, and Their Typically Developing Peers
Authors: Mughal Rabya , Wong Siu Sing , Dimitriou Dagmara , Halstead Elizabeth
Published: September 2021

Children with Foetal Alcohol Spectrum Disorders (FASD) and Autism Spectrum Disorders (ASD) experience significantly higher rates of sleep disturbances than their typically developing (TD) peers. Pre-sleep anxiety and waking emotional content is known to affect the content and frequency of nightmares, which can be distressing to children and caregivers. This is the first study to analyse nightmare frequency and content in FASD, and to assess its association with psychometric outcomes. Using online caregiver questionnaires, we assessed reports from 277 caregivers of children with ASD (n = 61), FASD (n = 112), and TD children (n = 104) using the Children’s Sleep Habits Questionnaire (CSHQ), the Child Behaviour Checklist (CBCL), the Spence Children’s Anxiety Scale (SCAS), and the Behaviour Rating Inventory for Executive Functioning (BRIEF). Within the ASD group, 40.3% of caregivers reported their children had nightmares. Within the FASD group, 73.62% of caregivers reported their children had nightmares, and within the TD group, 21.36% of caregivers reported their children had nightmares. Correlation analysis revealed significant associations between anxiety and nightmares, maladaptive behaviour and nightmares, and executive functioning and nightmares in the TD and FASD groups, but not ASD group. This paper adds to the emerging body of work supporting the need for sleep interventions as part of clinical practice with regard to children with ASD and FASD. As a relatively niche but important area of study, this warrants much needed further research.
Publisher’s Note to Launch Colorants—A Scientific Journal of Dyes, Pigments and Color Chemistry
Authors: Lin Shu-Kun
Published: September 2021

On 11 May 2010, the code ISSN 2079-6447 was obtained for Colorants following an application to the ISSN Center in Paris [...]
A Novel Simulation Platform for Underwater Data Muling Communications Using Autonomous Underwater Vehicles
Authors: Teixeira Filipe B. , Ferreira Bruno M. , Moreira Nuno , Abreu Nuno , Villa Murillo , Loureiro João P. , Cruz Nuno A. , Alves José C. , Ricardo Manuel , Campos Rui
Published: September 2021

Autonomous Underwater Vehicles (AUVs) are seen as a safe and cost-effective platforms for performing a myriad of underwater missions. These vehicles are equipped with multiple sensors which, combined with their long endurance, can produce large amounts of data, especially when used for video capturing. These data need to be transferred to the surface to be processed and analyzed. When considering deep sea operations, where surfacing before the end of the mission may be unpractical, the communication is limited to low bitrate acoustic communications, which make unfeasible the timely transmission of large amounts of data unfeasible. The usage of AUVs as data mules is an alternative communications solution. Data mules can be used to establish a broadband data link by combining short-range, high bitrate communications (e.g., RF and wireless optical) with a Delay Tolerant Network approach. This paper presents an enhanced version of UDMSim, a novel simulation platform for data muling communications. UDMSim is built upon a new realistic AUV Motion and Localization (AML) simulator and Network Simulator 3 (ns-3). It can simulate the position of the data mules, including localization errors, realistic position control adjustments, the received signal, the realistic throughput adjustments, and connection losses due to the fast SNR change observed underwater. The enhanced version includes a more realistic AML simulator and the antenna radiation patterns to help evaluating the design and relative placement of underwater antennas. The results obtained using UDMSim show a good match with the experimental results achieved using an underwater testbed. UDMSim is made available to the community to support easy and faster evaluation of underwater data muling oriented communications solutions and to enable offline replication of real world experiments.
Seasonal Activity of Fruit Bats in a Monoculture Rubber and Oil Palm Plantation in the Southern Philippines
Authors: Tanalgo Krizler C. , Sritongchuay Tuanjit , Hughes Alice C.
Published: September 2021

The increasing expansion of monoculture plantations poses a major threat to Asian tropical biodiversity. Yet, in many countries such as the Philippines, the ability of species to persist within plantations has never been explored. We studied the seasonal activity and response of fruit bats in two types of monocultural plantations (rubber and oil palm) in the Southern Philippines from 2016–17 for 12 months. Our mist-netting and monitoring data showed that both plantations can support cosmopolitan species of fruit bats (Cynopterus brachyotis, Eonycteris spelaea, Macroglossus minimus, Ptenochirus jagori, and Rousettus amplexicaudatus), yet a significant variation in the abundance and guild distribution between plantations was observed. Rubber hosted a higher bat abundance than oil palm, which may be influenced by better habitat structure of the matrix (e.g., presence of orchard and fruit plantations) and practices occurring in the rubber plantation. We find that, among seasonal climatic variables, temperature showed significant negative effects on fruit bat abundance. Our results suggest that although monoculture plantations host low diversity (i.e., richness and endemism) they still support generalists which are still ecologically important species. Furthermore, wildlife-friendly commercial plantation practices could both enhance economic growth and biodiversity conservation in the Philippines. Our data both provide the potential for long-term monitoring in the Philippines and highlight the need for more comprehensive monitoring of other bat functional groups and their ability to transverse plantations to provide a more in-depth understanding of the roles and impacts of plantations and other land-use changes.
Short-Term Prediction Methodology of COVID-19 Infection in South Korea
Authors: Ko Grace S. , Yoon Taeseon
Published: September 2021

The purpose of this study is to predict the short-term trend of the COVID-19 pandemic and give insights into effective response strategies. Based on the basic SIR model, a compartment method for modeling the course of an epidemic, the short-term infection change ratio md, is derived. The number of infected people can be predicted using this ratio. We calculated different md values on a weekly basis. As we tested different combinations of md, the prediction from the combination of md based on a week and md based on 4 weeks was found to be statistically reliable. According to our regression analysis, our approach has an explanatory power of 96%. However, this method could only predict 1 week ahead of current data. Thus, we use LSTM, a deep learning method applied for time series data, to forecast the trend 4 weeks ahead. The forecasted trends show that the number of infected people in South Korea will reach its peak a week after the writing of this work and start to gradually decline after that.
Diversity and Distribution of Culturable Thermus Species in Terrestrial Hot Springs of Southwestern Yunnan Province in China
Authors: Wang Yongxia , Xu Canhai , Han Long , Li Chengpeng , Xiao Wei , Cui Xiaolong
Published: September 2021

The Yunnan geothermal area has many neutral and alkalescent thermal springs. Members of the genus Thermus have been found in thermal environments. In this study, we attempted to cultivate numerically abundant Thermus species using a variety of different strategies. A total of 223 strains of Thermus-like bacteria were isolated from seventeen hot spring samples of four geothermal regions (Baoshan, Dali, Lincang and Dehong). These strains were classified into two genera, Thermus and Meiothermus, based on 16S rDNA. The optimal isolation temperature of the Thermus genus was 63–70 °C. The highest Thermus diversity was found at 63 °C. Thermus brockianus was a universal culturable bacterium in the four geothermal regions. The principal component analysis (PCA) showed that T. oshimai preferred to inhabit the hot springs of the Baoshan (Bs) geothermal region, and T. amyloliquefaciens dominated the Dali (Dl) geothermal region, whereas T. tengchongensis accumulated in the Lincang (Lc) and Dehong (Dh) geothermal regions. The results suggested that Thermus species had habitat-preferable characteristics among the four geothermal regions. The findings may help identify the niche from which Thermus strains can likely be isolated.
Farm Silage Facilities and Their Management for the Prevention of Anaerobic Bacteria Spore Contamination in Raw Milk
Authors: Abeni Fabio , Marino Rosanna , Petrera Francesca , Segati Giulia , Galli Andrea , Carminati Domenico
Published: September 2021

At feed-out, aerobic spoilage of silage enables an increase in anaerobic spore-forming bacteria (ANSB) that may enter the total mixed ration (TMR). The aim of our study was to understand whether in hot summers the silage structures and management may affect the level of ANSB in milk for long-ripening cheese production. A survey of silage facilities, management, and their relationships with silage, TMR, feces, and milk ANSB most probable number (MPN) content was conducted in the Po Valley during summer months. Silo type did not affect the mean ANSB, but only the wideness of their value distributions, with a narrow range for bags and a wider range for bunkers. The unloading equipment affected the ANSB count; the front-end loader with cutter was associated with a lower ANSB count—probably as a result of the reduced surface left after daily silage removal. Silo length and daily removed face width were the main factors affecting contamination of silage by spore-forming bacteria during summer, with longer silos and wider surface removal reducing ANSB contamination—probably as a consequence of reduced aerobic spoilage at the silage surface. The silage contamination by spore-forming bacteria within a log10 2 MPN g−1 allowed a low concentration of spore-forming bacteria at the farm bulk milk tank level. Fecal ANSB levels did not factor into the regression that explains the ANSB in farm milk. It has been found that silage facilities’ features and their management are an important first step to reduce the extent of ANSB contamination at the farm level.
Recent Updates and Advances in Winiwarter-Buerger Disease (Thromboangiitis Obliterans): Biomolecular Mechanisms, Diagnostics and Clinical Consequences
Authors: Fazeli Bahare , Ligi Daniela , Keramat Shayan , Maniscalco Rosanna , Sharebiani Hiva , Mannello Ferdinando
Published: September 2021

Thromboangiitis obliterans (TAO) or Buerger’s disease is a segmental inflammatory, thrombotic occlusive peripheral vascular disease with unknown aetiology that usually involves the medium and small-sized vessels of young male smokers. Due to its unknown aetiology and similarities with atherosclerosis and vasculitis, TAO diagnosis is still challenging. We aimed to review the status of biomolecular and laboratory para-clinical markers in TAO compared to atherosclerosis and vasculitis. We reported that, although some biomarkers might be common in TAO, atherosclerosis, and vasculitis, each disease occurs through a different pathway and, to our knowledge, there is no specific and definitive marker for differentiating TAO from atherosclerosis or vasculitis. Our review highlighted that pro-inflammatory and cell-mediated immunity cytokines, IL-33, HMGB1, neopterin, MMPs, ICAM1, complement components, fibrinogen, oxidative stress, NO levels, eNOS polymorphism, adrenalin and noradrenalin, lead, cadmium, and homocysteine are common markers. Nitric oxide, MPV, TLRs, MDA, ox-LDL, sST2, antioxidant system, autoantibodies, and type of infection are differential markers, whereas platelet and leukocyte count, haemoglobin, lipid profile, CRP, ESR, FBS, creatinine, d-dimer, hypercoagulation activity, as well as protein C and S are controversial markers. Finally, our study proposed diagnostic panels for laboratory differential diagnosis to be considered at first and in more advanced stages.
Impact of Three Different Algorithms for the Screening of SSc-PAH and Comparison with the Decisions of a Multidisciplinary Team
Authors: Coirier Valentin , Chabanne Céline , Jouneau Stéphane , Belhomme Nicolas , Ballerie Alice , Cazalets Claire , Sobanski Vincent , Hachulla Éric , Jégo Patrick , Lescoat Alain
Published: September 2021

Background: to compare three existing screening algorithms of pulmonary arterial hypertension (PAH) in systemic sclerosis (SSc) with the results of a multidisciplinary team (MDT) meeting from a tertiary center. Methods: we conducted a monocentric longitudinal study from 2015 to 2018. All patients with SSc according to LeRoy’s classification were eligible. Patients were excluded in the case of missing data required by any of the three screening algorithms. The algorithms were applied for each patient at inclusion. Right heart catheterization (RHC) was performed based on the MDT decision. MDT members were all blinded from the results of the three algorithms regarding RHC recommendations. The RHC recommendations of each algorithm were compared with the MDT decision, and the impact on diagnosis and management was evaluated. Results: 117 SSc patients were consecutively included in the study, and 99 had follow-up data over the three-year duration of the study (10 deaths). Among the 117 patients, the MDT suggested RHC for 16 patients (14%), DETECT algorithm for 28 (24%), ASIG for 48 (41%) and ESC/ERS 2015 for 20 (17%). Among the 16 patients who had RHC, SSc-PAH was diagnosed in seven. Among patients with an initial recommendation of RHC based on at least one algorithm but not according to the MDT meeting, no SSc-PAH was diagnosed during the three-year follow-up. Results were unchanged when the new 2018 definition of PAH was applied instead of the previous definition. Conclusion: a MDT approach appears interesting for the screening of SSc-PAH, with a significant reduction of RHC performed in comparison with dedicated algorithms. The specific relevance of a MDT for the management and follow-up of patients with RHC recommended by existing algorithms but with no PAH warrants further studies.
Whole-Body Radioiodine Effective Half-Life in Patients with Differentiated Thyroid Cancer
Authors: Klain Michele , Nappi Carmela , De Risi Marina , Piscopo Leandra , Volpe Fabio , Manganelli Mariarosaria , Caiazzo Elisa , Bianco Davide , Schlumberger Martin , Cuocolo Alberto
Published: September 2021

Background: Radioactive 131I (RAI) therapy is used in patients with differentiated thyroid cancer (DTC) after total thyroidectomy for remnant ablation, adjuvant treatment or treatment of persistent disease. 131I retention data, which are used to indicate the time at which a 131I treated DTC patient can be released from the hospital, may bring some insights regarding clinical factors that prolong the length of hospitalization. The aim of this study was to investigate the 131I whole-body retention in DTC patients during 131I therapy. Methods: We monitored 166 DTC patients to follow the 131I whole-body retention during 131I therapy with a radioactivity detector fixed on the ceiling of each protected room. A linear regression fit permitted us to estimate the whole-body 131I effective half-life in each patient, and a relationship was sought between patients’ clinical characteristics and whole-body effective 131I half-life. Results: The effective 131I half-life ranged from 4.08 to 56.4 h. At multivariable analysis, longer effective 131I half-life was related to older age and extensive extra-thyroid disease. Conclusions: 131I effective half-life during 131I treatment in DTC patients is highly variable among patients and is significantly longer in older and in patients with RAI uptake in large thyroid remnants or in extrathyroidal disease that significantly prolongs the whole-body retention of 131I.
A Systematic Review of Single-Case Research on Science-Teaching Interventions to Students with Intellectual Disability or Autism Spectrum Disorder
Authors: Iatraki Georgia , Soulis Spyridon-Georgios
Published: September 2021

The present study records and evaluates science-teaching interventions for students with intellectual disability (ID) or Autism Spectrum Disorder (ASD) at all education levels, for better access to the general curriculum. We conducted a systematic review of the primary research literature published from 2008 to 2018. A total of 24 single-case designs were identified through determined inclusion criteria. The articles were coded based on specific variables, and they were analyzed as a descriptive synthesis. A synthesis was then approached and the PND effect size was calculated. The study concludes that using systematic instruction with supplemental practices and technology support in teaching science to students with ID or ASD will allow them to make more meaningful natural-world connections through their learning. In addition, designing postsecondary programs for this population will contribute to building a society with equal participation and progress. The necessity for continuous research in teaching science to students with ID or ASD is also emphasized.
Melatonin as an Add-On Treatment of COVID-19 Infection: Current Status
Authors: Brown Gregory M. , Pandi-Perumal Seithikurippu R. , Pupko Harold , Kennedy James L. , Cardinali Daniel P.
Published: September 2021

This brief review was written to provide a perspective on the flurry of reports suggesting that melatonin can be an important add-on therapy for COVID-19. Despite the passage of more than 60 years since its discovery and much evidence representing the contrary, there has been great reluctance to conceive melatonin as anything other than a hormone. Many other body chemicals are known to have multiple roles. Melatonin was first shown to be a hormone derived from the pineal gland, to be actively synthesized there only at night, and to act on targets directly or via the G-protein-coupled receptors (GPCRs) superfamily. It is of note that over 40 years ago, it was also established that melatonin is present, synthesized locally, and acts within the gastrointestinal tract. A wider distribution was then found, including the retina and multiple body tissues. In addition, melatonin is now known to have non-hormonal actions, acting as a free radical scavenger, an antioxidant, and as modulating immunity, dampening down innate tissue responses to invaders while boosting the production of antibodies against them. These actions make it a potentially excellent weapon against infection by the SARS-CoV-2 virus. Early published results support that thesis. Recently, a randomized controlled study reported that low doses of melatonin significantly improved symptoms in hospitalized COVID-19 patients, leading to more rapid discharge with no side effects, while significantly decreasing levels of CRP, proinflammatory cytokines, and modulating dysregulated genes governing cellular and humoral immunity. It is now critical that these trials be repeated, with dose-response studies conducted and safety proven. Numerous randomized controlled trials are ongoing, which should complete those objectives while also allowing for a more thorough evaluation of the mechanisms of action and possible applications to other severe diseases.
Analysis of Korean Peninsula Earthquake Network Based on Event Shuffling and Network Shuffling
Authors: Min Seungsik , Lim Gyuchang
Published: September 2021

In this work, a Korean peninsula earthquake network, constructed via event-sequential linking known as the Abe–Suzuki method, was investigated in terms of network properties. A significance test for these network properties was performed via comparisons with those of two random networks, constructed from two approaches, that is, EVENT (SEQUENCE) SHUFFLING and NETWORK (MATRIX) SHUFFLING. The Abe–Suzuki earthquake network has a clear difference from the two random networks. However, the two shuffled networks exhibited completely different functions, and even some network properties for one shuffled datum are significantly high and those of the other shuffled data are low compared to actual data. For most cases, the event-shuffled network showed a functional similarity to the real network, but with different exponents/parameters. This result strongly claims that the Korean peninsula earthquake network has a spatiotemporal causal relation. Additionally, the Korean peninsula network properties are mostly similar to those found in previous studies on the US and Japan. Further, the Korean earthquake network showed strong linearity in a specific range of spatial resolution, that is, 0.20°~0.80°, implying that macroscopic properties of the Korean earthquake network are highly regular in this range of resolution.
Modeling the Price Volatility of Cassava Chips in Thailand: Evidence from Bayesian GARCH-X Estimates
Authors: Singvejsakul Jittima , Chaovanapoonphol Yaovarate , Limnirankul Budsara
Published: September 2021

Thailand is a significant global exporter of cassava, of which cassava chips are the main export products. Moreover, China was the most important export market for Thailand from 2000 to 2020. However, during that period, Thailand confronted fluctuations in the cassava product price, and cassava chips were a product with significant price volatility, adapting to changes in export volumes. This study aims to analyze the volatility of the price of cassava chips in Thailand from 2010 to 2020. The data were collected monthly from 2010 to 2020, including the price of cassava chips in Thailand (Y), the volume of cassava China imported from Thailand (X1), the price of the cassava chips that China imported from Thailand (X2), the price of the cassava starch that China imported from Thailand (X3), the substitute crop price for maize (X4), the substitute crop price for wheat (X5), and Thailand’s cassava product export volume (X6). The volatility and the factors affecting the volatility in the price of cassava chips were calculated using Bayesian GARCH-X. The results indicate that the increase in X1, X2, X3, X4, and X6 led to an increase in the rate of change in cassava chip price volatility. On the other hand, if the substitute crop price for wheat (X5) increases, then the rate of change in the volatility of the cassava chip price decreases. Therefore, the government’s formulation of an appropriate cassava policy should take volatility and the factors affecting price volatility into account. Additionally, the government’s formulation of agricultural policy needs to consider Thailand’s macro-environmental factors and its key trading partners, especially when these environmental factors signal changes in the price volatility of cassava.