bluuebunny/crossref_metadata_2025 huggingface dataset:
Assessing the impact of wildfires on water quality using satellite remote sensing: the Lake Baikal case study
Authors: Pinardi Monica , Stroppiana Daniela , Caroni Rossana , Parigi Lorenzo , Tellina Giulio , Free Gary , Giardino Claudia , Albergel Clément , Bresciani Mariano
Published: March 2023

Lakes have been observed as sentinels of climate change. In the last decades, global warming and increasing aridity has led to an increase in both the number and severity of wildfires. This has a negative impact on lake catchments by reducing forest cover and triggering cascading effects in freshwater ecosystems. In this work we used satellite remote sensing to analyse potential fire effects on lake water quality of Lake Baikal (Russia), considering the role of runoff and sediment transport, a less studied pathway compared to fire emissions transport. The main objectives of this study were to analyse time series and investigate relationships among fires (i.e., burned area), meteo-climatic parameters and water quality variables (chlorophyll-a, turbidity) for the period 2003–2020. Because Lake Baikal is oligotrophic, we expected detectable changes in water quality variables at selected areas near the three mains tributaries (Upper Angara, Barguzin, Selenga) due to river transport of fire-derived burned material and nutrients. Time series analysis showed seasonal (from April to June) and inter-annual fire occurrence, precipitation patterns (high intensity in summer) and no significant temporal changes for water quality variables during the studied periods. The most severe wildfires occurred in 2003 with the highest burned area detected (36,767 km2). The three lake sub-basins investigated have shown to respond differently according to their morphology, land cover types and meteo-climatic conditions, indicating their importance in determining the response of water variables to the impact of fires. Overall, our finding suggests that Lake Baikal shows resilience in the medium-long term to potential effects of fires and climate change in the region.
The effects of physical activity on glutamate neurotransmission in neuropsychiatric disorders
Authors: Baskerville Richard , McGrath Thomas , Castell Lindy
Published: March 2023

Physical activity (PA) is an effective way of increasing cognitive and emotional health and counteracting many psychiatric conditions. Numerous neurobiological models for depression have emerged in the past 30 years but many struggle to incorporate the effects of exercise. The hippocampus and pre-frontal cortex (PFC) containing predominantly glutamate neurotransmission, are the centres of changes seen in depression. There is therefore increasing interest in glutamatergic systems which offers new paradigms of understanding mechanisms connecting physical activity, stress, inflammation and depression, not explained by the serotonin theories of depression. Similar hippocampal glutamate dysfunction is observed in many other neuropsychiatric conditions. Excitatory glutamate neurones have high functionality, but also high ATP requirements and are therefore vulnerable to glucocorticoid or pro-inflammatory stress that causes mitochondrial dysfunction, with synaptic loss, culminating in depressed mood and cognition. Exercise improves mitochondrial function, angiogenesis and synaptogenesis. Within the glutamate hypothesis of depression, the mechanisms of stress and inflammation have been extensively researched, but PA as a mitigator is less understood. This review examines the glutamatergic mechanisms underlying depression and the evidence of physical activity interventions within this framework. A dynamic glutamate-based homeostatic model is suggested whereby stress, neuroinflammation and PA form counterbalancing influences on hippocampal cell functionality, which manifests as depression and other neuropsychiatric conditions when homeostasis is disrupted.
Emerging new global soil governance structure in agrifood systems: Taking the “4 per 1,000” initiative as an example
Authors: Li Li , Song Xiaojing , Liu Yang , Chai Li
Published: March 2023

Food systems emit 21%-37% of the global greenhouse gases (GHGs). Soil degradation, accelerated by global warming, poses a threat to over 40% of the land surfaces, threatening food security. Keeping soils alive and healthy could not only play a part in food security, but also in sequestrating GHGs for climate mitigation. In 2015, the “4 per 1,000” Initiative was launched in Paris COP21, indicating that a “4‰” annual growth rate of the soil organic carbon sequestration could hold the temperature increase within 1.5°C−2°C. However, major GHG emitting countries haven't signed the 4‰ Initiative at national level. Political willingness need to be encouraged though institutional innovations in the global soil governance (GSG). This article conducts a comprehensive policy review for the 4‰ Initiative and attempts to develop the concept of global soil governance from an aspect of New Common But Differentiated Responsibility. The SOC sink targets reveal that countries like China, India, the UK, the US, and France take more pains than those like Australia, Russia, and Canada. A new “soil carbon rich” and “soil carbon poor” divide is perceived, which needs to be taken into the GSG as a restructuring motivation for setting a more practical and integrated framework. In that sense, some developed countries face similar challenges as the developing countries do, but could contribute more in finance and technology. Bandwagon of applying sustainable agricultural land management (SALM) methodology in carbon markets implies that soil-climate co-benefits get greater practical momentum with quantified trading platforms, which may stimulate potentiality if embodied in Article 6 of the Paris Agreement.
The effectiveness and influencing factors of the “Y” line technique in reducing the leg length discrepancy after total hip arthroplasty
Authors: Jin Wenshu , Sun Huaqiang , Duan Xudong , Gu Yange , Zhao Zhang , Yan Xinfeng
Published: March 2023

Objective

To introduce a surgical technique (the “Y” line technique) that will control leg length discrepancy (LLD) after total hip arthroplasty and to observe its effectiveness and influencing factors.

Methods

According to the inclusion and exclusion criteria, a total of 350 patients were selected in this study; 134 patients in whom used the “Y” line technique was used to control lower limb length were included in Group A and 166 patients treated with freehand methods to control lower limb length were included in Group B. A total of 50 patients in whom the standard anteroposterior x-ray of bilateral hips was taken preoperatively and in whom the “Y” line technique was used during the operation were included in Group C.

Results

The postoperative LLD of Group A was 4.74 mm (3.93), that of Group B was 5.85 mm (4.60), and that of Group C was 2 mm (1.00)—the difference was statistically significant (p < 0.001). There were significant statistical differences when comparisons were made between any two groups (p < 0.01). The distribution of postoperative LLD in Group A was better than that in Group B, and this factor was better in Group C than in Group A—the difference was statistically significant (p < 0.001). Severe unequal length rates of the lower extremities (LLD > 10 mm) were 5.97% (8/134) in Group A, 14.3% (24/166) in Group B, and 0% (0/50) in Group C—the difference was statistically significant (p < 0.001). There were significant differences between Group A and Group B and between Group B and Group C (p < 0.05), but there was no significant difference between Group A and Group C (p = 0.078).

Conclusion

The “Y” line technique, which does not increase the operating time and patient cost, can effectively reduce postoperative LLD. Insufficient internal rotation of the healthy lower extremity and the low projection position in the preoperative anteroposterior x-ray of the bilateral hips were important factors affecting the accuracy of the “Y” line technique.
Training to be a spinal endoscopic surgeon: What matters?
Authors: Xie Yizhou , Zhou Qun , Wang Yongtao , Feng Chengzhi , Fan Xiaohong , Yu Yang
Published: March 2023

Objective

Spinal endoscopic surgery has been promoted rapidly in the past decade, attracting an increasing number of young, dedicated surgeons. However, it has long been denounced for its long learning curve as a factor impeding the development of this state-of-the-art technique. The aim of the present study was to discover what really matters in the educational process of becoming a spinal endoscopic surgeon.

Methods

An online survey consisting of 14 compulsory questions was distributed in April and May 2022 through the First Chinese Spinal Endoscopic Surgeons Skills Competition. Reminders were sent to increase response rates.

Results

Of the 893 emails that were sent, we received 637 responses. A total of 375 (76.7%) surgeons most frequently used endoscopic techniques in their practices. Regardless of their different backgrounds, 284 (75.7%) surgeons thought it would be necessary for a young spinal endoscopic surgeon to perform 300 cases independently in order to become proficient, followed by 500 (n=43, 11.5%), 100 (n=40, 10.7%), and 1,000 (n=8, 2.1%) cases. According to the surgeons, the most difficult aspect of mastering the endoscopic technique is a disparate surgical view (n=255, 68%), followed by adaption to new instruments (n=86, 22.9%) and hand-eye coordination (n=34, 9.1%). The most helpful training method for helping the spinal endoscopic surgeons of younger generations improve is operating on simulation models or cadaver courses (n=216, 57.6%), followed by online or offline theoretical courses (n=67, 17.9%), acquiring opportunities during surgeries (n=51, 13.6%), and frequently participating in surgeries as an assistant (n=41, 10.9%).

Conclusion

From the perspective of surgeons, to be skilled in spinal endoscopic surgery means overcoming a steep learning curve. However, training systems should be given more attention to make them more accessible to younger surgeons so they can work on simulation models or take cadaver courses.
Search for the optimized and key nephrometry elements combination in retroperitoneal laparoscopic partial nephrectomy: A retrospective study
Authors: Jin Yanyang , Wang Mingshuai , Xing Nianzeng
Published: March 2023

Background

The nephrometry scoring system plays a key role in the preoperative evaluation of partial nephrectomy, and scoring systems based on anatomical characteristics have high similarity in scoring elements. Currently, there is little research on scoring systems related to retroperitoneal laparoscopic partial nephrectomy, and there is a lack of research on the combination of scoring elements, which requires further investigation.

Methods

We retrospectively analyzed the clinical records of 107 patients who underwent retroperitoneal laparoscopic partial nephrectomy conducted by a single operator at a single center. The score and scoring elements were generated based on imaging. The scoring elements of each scoring system and all combinations of two to five elements were extracted. The predictive ability of different score combinations was evaluated by AUC value, and the key parameters of the score were found by taking the intersection. A nomogram was constructed and evaluated.

Results

We observed that with an increase in scoring elements, the strongest combination of elements did not significantly increase the predictive ability of warm ischemia time (P>0.05), postoperative complications (P>0.05), and trifecta achievement (P>0.05). The combination of the maximum tumor diameter and the distance between tumor and collecting system or renal sinus had a good comprehensive predictive ability, and there is no significant difference with the traditional score (P>0.05). The nomogram generated according to this combination has an excellent prediction ability for predicting whether obtain trifecta of partial nephrectomy.

Conclusions

Within the range of two to five elements, the critical degree of elements is more important than the number of elements. The maximum tumor diameter and the distance between the tumor and the collecting system or renal sinus was the key element of the prediction ability.
Effects of tiletamine-zolazepam vs. propofol on peri-induction intraocular pressure in dogs: A randomized, masked crossover study
Authors: McIver Katharine A. , Boveland Shannon D. , Clark-Price Stuart C. , Hofmeister Erik H.
Published: March 2023

Introduction

Anesthesia induction agents have the potential to cause severe ocular side effects, resulting in lasting damage to the eye.

Objectives

The purpose of this study is to determine the effects of tiletamine—zolazepam on IOP compared to propofol when they are used as an induction agent in normal healthy dogs.

Methods

Twenty healthy adult client owned dogs weighing 22.2 ± 7.6 kg were selected for the study. In a randomized order, all dogs received tiletamine-zolazepam 5 mg/kg IV or propofol 8 mg/kg IV titrated to effect without premedication. Washout between each treatment was at least seven days. IOP measurements were obtained at four time points: baseline, post-induction, post-intubation, and after recovery using applanation tonometry. No additional procedures were performed. After normality of the data was determined, a linear mixed model was built with time, eye, treatment and all interactions of those variables as fixed effects and subject as a random effect.

Results

There was no significant difference for age, body weight, drug dose, baseline IOP, and recovery IOP between treatments. Average IOP measurements remained within the normal range of 15-25 mmHg at these time points. However, IOP was significantly less elevated by the tiletamine-zolazepam treatment vs. propofol at the post-induction (mean difference: −4.7 ± 4.6 [95%CI −6.8 to −2.5]) and the post-intubation (mean difference: −4.4 ± 4.6 [95%CI −6.5 to −2.2]) time points.

Clinical significance

Dogs receiving tiletamine-zolazepam for anesthetic induction had a significantly less elevated IOP at induction and intubation compared to dogs receiving propofol.
Clinical review of high-flow nasal oxygen therapy in human and veterinary patients
Authors: Whitney Joanna , Keir Iain
Published: March 2023

Oxygen therapy is the first-line treatment for hypoxemic acute respiratory failure. In veterinary medicine this has traditionally been provided via mask, low-flow nasal oxygen cannulas, oxygen cages and invasive positive pressure ventilation. Traditional non-invasive modalities are limited by the maximum flow rate and fraction of inspired oxygen (FiO2) that can be delivered, variability in oxygen delivery and patient compliance. The invasive techniques are able to provide higher FiO2 in a more predictable manner but are limited by sedation/anesthesia requirements, potential complications and cost. High-flow nasal oxygen therapy (HFNOT) represents an alternative to conventional oxygen therapy. This modality delivers heated and humidified medical gas at adjustable flow rates, up to 60 L/min, and FiO2, up to 100%, via nasal cannulas. It has been proposed that HFNOT improves pulmonary mechanics and reduces respiratory fatigue via reduction of anatomical dead space, provision of low-level positive end-expiratory pressure (PEEP), provision of constant FiO2 at rates corresponding to patient requirements and through improved patient tolerance. Investigations into the use of HFNOT in veterinary patients have increased in frequency since its clinical use was first reported in dogs with acute respiratory failure in 2016. Current indications in dogs include acute respiratory failure associated with pulmonary parenchymal disease, upper airway obstruction and carbon monoxide intoxication. The use of HFNOT has also been advocated in certain conditions in cats and foals. HFNOT is also being used with increasing frequency in the treatment of a widening range of conditions in humans. Although there remains conflict regarding its use and efficacy in some patient groups, overall these reports indicate that HFNOT decreases breathing frequency and work of breathing and reduces the need for escalation of respiratory support. In addition, they provide insight into potential future veterinary applications. Complications of HFNOT have been rarely reported in humans and animals. These are usually self-limiting and typically result in lower morbidity and mortality than those associated with invasive ventilation techniques.
Effects of emamectin benzoate on pharmacokinetic profiles, bioavailability, and serum biochemical indices in crucian carp (Carassius carassius) following oral administration with multiple dosage levels
Authors: Sun RuYu , Liu YongTao , Ai XiaoHui , Du XiangXuan , Zhang XiaoYi
Published: March 2023

The aim of this investigation was to explore the effect of three different dose levels of emamectin benzoate (EMB) (50, 200, and 500 μg/kg bw) on pharmacokinetic characterizations, tissue distribution patterns, absolute bioavailability, and serum biochemical indices in crucian carp following oral administration at 22 ± 2°C, respectively. We further calculated the relevant parameters by detecting the concentration of EMB in the crucian carp by the ultra-HPLC detection method. The results showed that Cmax, AUC, and T1/2z after oral administration showed a dose-dependent increase in plasma. Secondly, EMB has a long mean flow time (MRT) (51.88, 67.04, and 78.00 h, respectively). In conclusion, the elimination of the drug is slow, and the higher dose used, the slower elimination is. The distribution in various tissues of crucian carp was analyzed. The order of EMB levels in tissues of crucian carp was liver > gill > kidney > muscle plus skin > plasma. As for pharmacokinetic parameters in tissues, Cmax, AUC, and T1/2z in tissues had a similar pattern as that in plasma. The absolute oral bioavailability of EMB (F%) in crucian carp was calculated to be approximately 52.70%. The serum biochemical indices including ALT and AST in experimental groups exhibited significant differences (P < 0.05) compared with the control group while ALB, ALP, TP, T-CHO, BUN, GRE, and GLU were not significantly different to the control group (P > 0.05). Briefly, EMB has the characteristic of quick absorption and slow elimination in crucian carp with a high bioavailability by PO route in crucian carp.
Filling gaps in animal welfare assessment through metabolomics
Authors: Fabrile Maria Pia , Ghidini Sergio , Conter Mauro , Varrà Maria Olga , Ianieri Adriana , Zanardi Emanuela
Published: March 2023

Sustainability has become a central issue in Italian livestock systems driving food business operators to adopt high standards of production concerning animal husbandry conditions. Meat sector is largely involved in this ecological transition with the introduction of new label claims concerning the defense of animal welfare (AW). These new guarantees referred to AW provision require new tools for the purpose of authenticity and traceability to assure meat supply chain integrity. Over the years, European Union (EU) Regulations, national, and international initiatives proposed provisions and guidelines for assuring AW introducing requirements to be complied with and providing tools based on scoring systems for a proper animal status assessment. However, the comprehensive and objective assessment of the AW status remains challenging. In this regard, phenotypic insights at molecular level may be investigated by metabolomics, one of the most recent high-throughput omics techniques. Recent advances in analytical and bioinformatic technologies have led to the identification of relevant biomarkers involved in complex clinical phenotypes of diverse biological systems suggesting that metabolomics is a key tool for biomarker discovery. In the present review, the Five Domains model has been employed as a vademecum describing AW. Starting from the individual Domains—nutrition (I), environment (II), health (III), behavior (IV), and mental state (V)—applications and advances of metabolomics related to AW setting aimed at investigating phenotypic outcomes on molecular scale and elucidating the biological routes most perturbed from external solicitations, are reviewed. Strengths and weaknesses of the current state-of-art are highlighted, and new frontiers to be explored for AW assessment throughout the metabolomics approach are argued. Moreover, a detailed description of metabolomics workflow is provided to understand dos and don'ts at experimental level to pursue effective results. Combining the demand for new assessment tools and meat market trends, a new cross-strategy is proposed as the promising combo for the future of AW assessment.
Tendencja do hiperbolizacji w kreowaniu rzeczywistości we współczesnych
tekstach kultury na przykładzie internetowych artykułów informacyjnych
Authors: Galilej Cecylia
Published: March 2023

The subject matter of the analysis in this article is the phenomenon of hyperbolisation in mass media communication, which is often encountered in the public sphere. A hyperbola is a verbal construction (a rhetorical figure as well as a clue) with a special linguistic potential, often used for advertising, expressive, commentary, and ludic purposes. The impression of semanƟtic excess and the process of creating the convention of the aesthetics of overstatement and exaggerayion in the media message are ensured by specific linguistic means, including: appropriate types of lexis,
phraseologisms, diverse metaphors, morphological measures, and a supra-linguistic context. It turns out that hyperbolisation is one of the linguistic determinants of mass culture. It makes the media message more attractive, strengthens its persuasive power, is emotional, fresh, and linguistically original. It also transmits certain values: cognitive, utilitarian, hedonistic, aestheyic, vital, ethical
Asian Affective and Emotional State (A2ES) Dataset of ECG and PPG for Affective Computing Research
Authors: Ab. Aziz Nor Azlina , K. Tawsif , Ismail Sharifah Noor Masidayu Sayed , Hasnul Muhammad Anas , Ab. Aziz Kamarulzaman , Ibrahim Siti Zainab , Abd. Aziz Azlan , Raja J. Emerson
Published: March 2023

Affective computing focuses on instilling emotion awareness in machines. This area has attracted many researchers globally. However, the lack of an affective database based on physiological signals from the Asian continent has been reported. This is an important issue for ensuring inclusiveness and avoiding bias in this field. This paper introduces an emotion recognition database, the Asian Affective and Emotional State (A2ES) dataset, for affective computing research. The database comprises electrocardiogram (ECG) and photoplethysmography (PPG) recordings from 47 Asian participants of various ethnicities. The subjects were exposed to 25 carefully selected audio–visual stimuli to elicit specific targeted emotions. An analysis of the participants’ self-assessment and a list of the 25 stimuli utilised are also presented in this work. Emotion recognition systems are built using ECG and PPG data; five machine learning algorithms: support vector machine (SVM), k-nearest neighbour (KNN), naive Bayes (NB), decision tree (DT), and random forest (RF); and deep learning techniques. The performance of the systems built are presented and compared. The SVM was found to be the best learning algorithm for the ECG data, while RF was the best for the PPG data. The proposed database is available to other researchers.
A Dynamic Programming Approach to Ecosystem Management
Authors: Rosso Alessandra , Venturino Ezio
Published: March 2023

We propose a way of dealing with invasive species or pest control in agriculture. Ecosystems can be modeled via dynamical systems. For their study, it is necessary to establish their possible equilibria. Even a moderately complex system exhibits, in general, multiple steady states. Usually, they are related to each other through transcritical bifurcations, i.e., the system settles to a different equilibrium when some bifurcation parameter crosses a critical threshold. From a situation in which the pest is endemic, it is desirable to move to a pest-free point. The map of the system’s equilibria and their connections via transcritical bifurcations may indicate a path to attain the desired state. However, to force the parameters to cross the critical threshold, some human action is required, and this effort has a cost. The tools of dynamic programming allow the detection of the cheapest path to reach the desired goal. In this paper, an algorithm for the solution to this problem is illustrated.
A Tri-Model Prediction Approach for COVID-19 ICU Bed Occupancy: A Case Study
Authors: Stasinos Nikolaos , Kousis Anestis , Sarlis Vangelis , Mystakidis Aristeidis , Rousidis Dimitris , Koukaras Paraskevas , Kotsiopoulos Ioannis , Tjortjis Christos
Published: March 2023

The impact of COVID-19 and the pressure it exerts on health systems worldwide motivated this study, which focuses on the case of Greece. We aim to assist decision makers as well as health professionals, by estimating the short to medium term needs in Intensive Care Unit (ICU) beds. We analyse time series of confirmed cases, hospitalised patients, ICU bed occupancy, recovered patients and deaths. We employ state-of-the-art forecasting algorithms, such as ARTXP, ARIMA, SARIMAX, and Multivariate Regression models. We combine these into three forecasting models culminating to a tri-model approach in time series analysis and compare them. The results of this study show that the combination of ARIMA with SARIMAX is more accurate for the majority of the investigated regions in short term 1-week ahead predictions, while Multivariate Regression outperforms the other two models for 2-weeks ahead predictions. Finally, for the medium term 3-weeks ahead predictions the Multivariate Regression and ARIMA with SARIMAX show the best results. We report on Mean Absolute Percentage Error (MAPE), Root Mean Squared Error (RMSE), R-squared (R2), and Mean Absolute Error (MAE) values, for one-week, two-week and three-week ahead predictions for ICU bed requirements. Such timely insights offer new capabilities for efficient management of healthcare resources.
An Observer-Driven Distributed Consensus Braking Control Method for Urban Railway Trains with Unknown Disturbances
Authors: Chen Bin , Zhang Rui , Zhou Feng , Du Wei
Published: March 2023

An urban railway train is a braking power-distributed system consisting of multiple carriages, which is becoming a powerful transportation tool to alleviate traffic congestion within cities as well as across cities. It is critical to control an urban railway train synchronously for improving braking performances, but challenging to be achieved due to strong coupling, unknown dynamics, and disturbances. This paper proposes an observer-driven distributed consensus braking control method for an urban railway train. Specifically, according to the data intersection among carriages, a distributed consensus braking controller is designed to make the velocity of each carriage converge to the desired braking curve. A sliding mode disturbance observer is then developed to estimate the non-linear coupling force and disturbances. The estimation value is utilized to compensate for the distributed consensus braking control law. Moreover, the potential fields are introduced to guarantee that the distances between any two neighbouring carriages are stabilized in a safe range. The effectiveness of the developed control strategy is firstly authenticated via the Lyapunov stability theory and then validated via numerical comparative simulations.
A Review on Vibration Control Strategies Using Magnetorheological Materials Actuators: Application Perspective
Authors: Masa’id Aji , Lenggana Bhre Wangsa , Ubaidillah U. , Susilo Didik Djoko , Choi Seung-Bok
Published: March 2023

Magnetorheological (MR) materials are a group of smart materials used in new technologies with controlled reliability. The development of these materials is expanding, starting from MR fluids, elastomers, grease, and gel. This large number of material types further expands the various applications of MR materials as a creative technology to support performance enhancement. For example, MR fluid is used to improve the performance of shock absorbers such as vehicle suspension, the damping of building structures, and polishing of the workpiece. MR elastomers are used for engine mounting, insulation base, and many other applications with intelligent material properties such as stiffness controllability. However, there are still complexities in the practical implementation of the control system beyond reliability. Many previous studies have focused on the performance improvement and reliability of MR materials as smart materials for application devices and systems. In this review article, the specific discussion related to vibration control strategies in MR material-based systems was thoroughly investigated. To discuss this point, many MR applications including transportation system and vibration isolation were adopted using different types of control strategies. Many different control strategies that have been used for MR applications such as fuzzy logic control, optimal control, and skyhook control are discussed in-depth in terms of the inherent control characteristics of merits and demerits.
Design of Device for Optical Luminescent Diagnostic of the Seeds Infected by Fusarium
Authors: Moskovskiy Maksim N. , Belyakov Mikhail V. , Dorokhov Alexey S. , Boyko Andrey A. , Belousov Sergey V. , Noy Oleg V. , Gulyaev Anatoly A. , Akulov Sergey I. , Povolotskaya Anastasia , Efremenkov Igor Yu.
Published: March 2023

The development and application of optical luminescent methods and devices will help obtain information quickly and objectively about the level of Fusarium infection of agricultural plants. For the previously obtained ranges, the spectral characteristics of excitation and luminescence of wheat, barley, and oats of various degrees of infection were measured. The obtained dependences of flows on infection were approximated by linear regression models and relative sensitivities were determined. For wheat and barley, it is advisable to determine the degree of infection by the ratio of flows Φλ1/Φλ2, which makes it possible to calibrate the measuring device in relative units and increase its sensitivity. A method for determining the degree of infected seeds with Fusarium was developed. After the seeds are placed in a light-tight chamber, they are excited by radiation, and photoluminescence is recorded. The electrical signal from the radiation receiver is amplified and processed accounting for previously obtained calibration curves. In the universal device that measures the infection of wheat, barley, and oats seeds, it is necessary to have three radiation sources: 362 nm, 424 nm, and 485 nm. Based on the energy efficiency criteria, optimal LEDs and photodiodes, as well as a microcontroller, switches, operational amplifiers, a display, and other components of the device, were selected.
An Overview of Comet Assay Application for Detecting DNA Damage in Aquatic Animals
Authors: Jiang Nan , Naz Saima , Ma Yulin , Ullah Qudrat , Khan Muhammad Zahoor , Wang Jiaqi , Lu Xuan , Luosang Dun-Zhu , Tabassum Sadia , Chatha Ahmad Manan Mustafa , Basang Wang-Dui
Published: March 2023

This review discusses several research studies that employed comet assay to evaluate the environmental impact of genotoxins in aquatic environments. It focuses on in vivo and in situ studies of aquatic animals. New chemicals are being added each year to the existing burden of toxic substances in the environment. Excessive agricultural and industrial activities adversely affect biodiversity, threatening the survival of species in a particular habitat, as well as posing disease risks to humans. Some of the chemicals, e.g., pesticides and heavy metals, may be genotoxic to the sentinel species and/or to non-target species, causing deleterious effects in somatic or germ cells. Comet assay is a quick, sensitive, and low-cost technique for detecting DNA strand breakage. However, the comet assay has much more to offer than being an assay for testing DNA strand breaks in animal organs. The use of repair enzymes increases the range of DNA lesions that can be detected with the assay. Comparing data from studies that employed different approaches, such as empirical scoring or comet tail lengths, comet assay is one of the challenging techniques to be utilized in environmental studies. The relative amount of DNA in the comet tail indicates DNA break intensity. The assay has been modified to detect various base alterations by including the digestion of nucleoids with a lesion-specific endonuclease. The determination of DNA damage in these indicator species using the comet test would thus offer information on the genotoxic potential of their habitat at an early stage. This would enable intervention techniques to prevent or mitigate adverse health impacts in sentinel animals and humans.
The Use of Plant Growth Promoting Rhizobacteria to Reduce Greenhouse Gases in Strawberry Cultivation under Different Soil Moisture Conditions
Authors: Paliwoda Dominika , Mikiciuk Grzegorz , Mikiciuk Małgorzata , Miller Tymoteusz , Kisiel Anna , Sas-Paszt Lidia , Kozioł Agnieszka , Brysiewicz Adam
Published: March 2023

One of the main causes of climate change is the emission of GHGs, and one of the sources for the generation of such gasses is agriculture via plant production. Considering the foregoing, a study was conducted to assess PGPRs in strawberry cultivation which were able to limit GHG emissions. The first experimental factor was the inoculation of plant roots with the Bacillus sp. strains DLGB3, DKB26, DKB58, and DKB 84; the Pantoea sp. strains DKB63, DKB64, DKB65, and DKB68; Azotobacter sp. AJ 1.2; and Pseudomonas sp. PJ 1.1. The second experimental factor constituted the different moisture levels of the growth substrate. In the experiment, emissions of NH3, CO2, N2O, and CH4 were measured. In light of the conducted research, five strains were selected (Azotobacter sp. AJ 1.2; Pantoea sp. DKB64, DKB63, and DKB68; and Pseudomonas sp. strain PJ 1.1) that showed the greatest potential for reducing GHG emissions depending on the prevailing environmental conditions. The application of the tested bacterial strains under different moisture conditions in the substrate either reduced or did not affect GWP. This research on PGPR, which was conducted to select strains of rhizosphere bacteria that would be able to reduce GHG emissions, may form the basis for creating an inoculum and can be employed as an effective strategy for mitigating certain abiotic stresses.
Effects of Training for First Milking Involving Positive Tactile Stimulation on Post-Calving Maternal Behaviors in Primiparous Gyr Dairy Cows
Authors: Vicentini Rogério Ribeiro , El Faro Lenira , Ujita Aska , Ceballos Maria Camila , Negrão João Alberto , Sant’Anna Aline Cristina
Published: March 2023

In dairy systems with Zebu breeds, calves are not immediately separated from their dams after calving; consequently, maternal care and protective behavior are important, influencing both productive performance and stockpeople’s safety. Our objectives were to: (1) investigate the effects of a training protocol involving pre-calving positive stimulation, delivered prior to calving, on the maternal care of primiparous Gyr cows; and (2) evaluate the effects of this training protocol on maternal protective behavior towards handlers during the first calf handling. Primiparous dairy Gyr cows (n = 37) were allocated into two groups: training (n = 16) and control (n = 21). Animal behaviors were recorded in three periods: post-calving, first calf handling, and post-handling. Maternal protective behavior during calf handling was assessed from measures of aggressiveness, attention, displacement, and agitation. Calf latency to stand up (p < 0.01) and sex (p < 0.01) differed between the training and control groups. The training group had less touching (p = 0.03), more time not interacting with the calf (p = 0.03), tended to be less protective (p = 0.056), and moved less (p < 0.01) during the first handling of their calves. In conclusion, the primiparous dairy Gyr cows subjected to pre-calving training protocol displayed less maternal care and displacement during the first handling of their calves and tended to be less protective.
Egg Removal via Cloacoscopy in Three Dystocic Leopard Geckos (Eublepharis macularius)
Authors: Vetere Alessandro , Bigliardi Enrico , Masi Marco , Rizzi Matteo , Leandrin Elisa , Di Ianni Francesco
Published: March 2023

Dystocia is a multifactorial, life-threatening condition commonly affecting pet reptiles. Treatment for dystocia can be either medical or surgical. Medical treatment usually involves the administration of oxytocin, but in some species or, in some cases, this treatment does not work as expected. Surgical treatments such as ovariectomy or ovariosalpingectomy are resolutive, but invasive in small-sized reptiles. In this paper, we describe three cases of post ovulatory egg retention in three leopard geckos (Eublepharis macularius) successfully treated through a cloacoscopic removal of the retained eggs, after a non resolutive medical treatment. The intervention was fast, non-invasive, and no procedure-related adverse effects were noted. The problem relapsed six months later in one animal, and a successful bilateral ovariosalpingectomy was performed. Cloacoscopy should be considered a valuable, non-invasive tool for egg removal in dystocic leopard geckos when the egg is accessible to manipulation. Recrudescence or complications such as adhesions, oviductal rupture, or the presence of ectopic eggs should recommend surgical intervention.
Alterations in Fecal Microbiota Linked to Environment and Sex in Red Deer (Cervus elaphus)
Authors: Sun Yue , Yu Yanze , Guo Jinhao , Zhong Linqiang , Zhang Minghai
Published: March 2023

Gut microbiota play an important role in impacting the host’s metabolism, immunity, speciation, and many other functions. How sex and environment affect the structure and function of fecal microbiota in red deer (Cervus elaphus) is still unclear, particularly with regard to the intake of different diets. In this study, non-invasive molecular sexing techniques were used to determine the sex of fecal samples from both wild and captive red deer during the overwintering period. Fecal microbiota composition and diversity analyses were performed using amplicons from the V4–V5 region of the 16S rRNA gene sequenced on the Illumina HiSeq platform. Based on Picrust2 prediction software, potential function distribution information was evaluated by comparing the Kyoto Encyclopedia of Genes and Genome (KEGG). The results showed that the fecal microbiota of the wild deer (WF, n = 10; WM, n = 12) was significantly enriched in Firmicutes and decreased in Bacteroidetes, while the captive deer (CF, n = 8; CM, n = 3) had a significantly higher number of Bacteroidetes. The dominant species of fecal microbiota in the wild and captive red deer were similar at the genus level. The alpha diversity index shows significant difference in fecal microbiota diversity between the males and females in wild deer (p < 0.05). Beta diversity shows significant inter-group differences between wild and captive deer (p < 0.05) but no significant differences between female and male in wild or captive deer. The metabolism was the most important pathway at the first level of KEGG pathway analysis. In the secondary pathway of metabolism, glycan biosynthesis and metabolism, energy metabolism, and the metabolism of other amino acids were significantly different. In summary, these compositional and functional variations in the fecal microbiota of red deer may be helpful for guiding conservation management and policy decision-making, providing important information for future applications of population management and conservation.
Effects of Schisandra chinensis Polysaccharide-Conjugated Selenium Nanoparticles on Intestinal Injury in Mice
Authors: Du Hongxu , Tan Xiaoyan , Li Zhangxun , Dong Hong , Su Lijuan , He Zhengke , Ma Qi , Dong Shiqi , Ramachandran Mythili , Liu Juan , Cao Liting
Published: March 2023

Schisandra chinensis polysaccharide (SCP) is an experimental therapeutic for the treatment of intestinal injury. Selenium nanoparticle modification can improve the bioactivity of polysaccharides. In this study, SCP was firstly extracted and purified by a DEAE-52 column, then SCP-Selenium nanoparticles (SCP-Se NPs) were prepared, and the procedure was optimized. Thereafter, the obtained SCP-Se NPs were characterized by transmission electron microscope, X-ray diffraction, energy-dispersive X-ray spectroscopy, and Fourier transform infrared spectroscopy. The influence of different storage environments on the stability of colloidal SCP-Se NPs was also investigated. Finally, the therapeutic effects of SCP-Se NPs on LPS-induced intestinal inflammatory injuries in mice were evaluated. Results showed that the optimized SCP-Se NPs were amorphous, uniform, spherical particles with a diameter of 121 nm, and the colloidal solution was stable at 4 °C for at least 14 d. Moreover, SCP-Se NPs could more effectively alleviate LPS-induced diarrhea, intestinal tissue injury, and tight junction destruction and decrease the elevated expression levels of TNF-α, IL-1β, and IL-6 compared with SCP. These results demonstrate that SCP-Se NPs may alleviate LPS-induced enteritis through their anti-inflammatory effects, indicating that SCP-Se NPs can serve as a good candidate for preventing and treating enteritis in the livestock and poultry industry.
Morphology, Histology, and Histochemistry of the Digestive Tract of the Marbled Flounder Pseudopleuronectes yokohamae
Authors: Cho Jeong-Hyeon , Park Jin Woo , Ryu Yong-Woon , Kim Kang-Woong , Hur Sang-Woo
Published: March 2023

This study investigated the morphological, histological, and histochemical characteristics of the digestive tract of the marbled flounder (Pseudopleuronectes yokohamae). The relative length of the gut of the marbled flounder digestive tract was 1.54 ± 0.10 (n = 20), and it had a simple stomach and 6–9 pyloric caeca. The mucosal folds of the marbled flounder digestive tract exhibited a general branched morphology. The thickness and mucosal fold length of the intestinal muscularis externa showed similar aspects in all areas. The thickness of the intestinal muscularis externa was the thickest in the posterior intestine portion, and the length of mucosal folds was the longest in the anterior intestine portion. It was indicated that food digested by gastric acid in the stomach moves to the anterior portion (including pyloric caeca) and mid portion of the intestine, ensuring effective stimulation of cholecystokinin (CCK)-producing cells. In addition, the distribution pattern of CCK-producing cells in the intestine was very similar to that of mucus-secreting goblet cells. The CCK-producing cells and goblet cells in the marbled flounder were well-adapted to promote optimal control of the digestive process. Based on the morphological and histochemical studies, it was concluded that the marbled flounder displays a digestive tract comparable to that of fish species with carnivorous habits.
Effect of Maternal Gradient Nutritional Restriction during Pregnancy on Mammary Gland Development in Offspring
Authors: Dong Xusheng , Lin Xueyan , Hou Qiuling , Hu Zhiyong , Wang Yun , Wang Zhonghua
Published: March 2023

We aimed to investigate the effect of different levels of nutritional restriction on mammary gland development during the embryonic period by gradient nutritional restriction in pregnant female mice. We started the nutritional restriction of 60 female CD-1(ICR) mice from day 9 of gestation based on 100%, 90%, 80%, 70% and 60% of ad libitum intake. After delivery, the weight and body fat of the offspring and the mother were recorded (n = 12). Offspring mammary development and gene expression were explored by whole mount and qPCR. Mammary development patterns of in offspring were constructed using Sholl analysis, principal component analysis (PCA) and regression analysis. We found that: (1) Mild maternal nutritional restriction (90–70% of ad libitum intake) did not affect offspring weight, while body fat percentage was more sensitive to nutritional restriction (lower at 80% ad libitum feeding). (2) A precipitous drop in mammary development and altered developmental patterns occurred when nutritional restriction ranged from 80% to 70% of ad libitum intake. (3) Mild maternal nutritional restriction (90% of ad libitum intake) promoted mammary-development-related gene expression. In conclusion, our results suggest that mild maternal nutritional restriction during gestation contributes to increased embryonic mammary gland development. When maternal nutritional restriction reaches 70% of ad libitum intake, the mammary glands of the offspring show noticeable maldevelopment. Our results help provide a theoretical basis for the effect of maternal nutritional restriction during gestation on offspring mammary development and a reference for the amount of maternal nutritional restriction.
Monosodium Glutamate Perturbs Human Trophoblast Invasion and Differentiation through a Reactive Oxygen Species-Mediated Pathway: An In-Vitro Assessment
Authors: Mukherjee Indrani , Biswas Subhrajit , Singh Sunil , Talukdar Joyeeta , Alqahtani Mohammed S. , Abbas Mohamed , Nag Tapas Chandra , Mridha Asit Ranjan , Gupta Surabhi , Sharma Jai Bhagwan , Kumari Supriya , Dhar Ruby , Karmakar Subhradip
Published: March 2023

The overproduction of reactive oxygen species (ROS) has been associated with various human diseases. ROS exert a multitude of biological effects with both physiological and pathological consequences. Monosodium glutamate (MSG), a sodium salt of the natural amino acid glutamate, is a flavor-enhancing food additive, which is widely used in Asian cuisine and is an ingredient that brings out the “umami” meat flavor. MSG consumption in rats is associated with ROS generation. Owing to its consumption as part of the fast-food culture and concerns about its possible effects on pregnancy, we aimed to study the impact of MSG on placental trophoblast cells. MSG exposure influenced trophoblast invasion and differentiation, two of the most critical functions during placentation through enhanced production of ROS. Similar findings were also observed on MSG-treated placental explants, as confirmed by elevated Nrf2 levels. Ultrastructural studies revealed signs of subcellular injury by MSG exposure. Mechanistically, MSG-induced oxidative stress with endoplasmic reticulum stress pathways involving Xbp1s and IRE1α was observed. The effect of MSG through an increased ROS production indicates that its long-term exposure might have adverse health effect by compromising key trophoblast functions.
Molecular Hydrogen: From Molecular Effects to Stem Cells Management and Tissue Regeneration
Authors: Artamonov Mikhail Yu. , Martusevich Andrew K. , Pyatakovich Felix A. , Minenko Inessa A. , Dlin Sergei V. , LeBaron Tyler W.
Published: March 2023

It is known that molecular hydrogen is a relatively stable, ubiquitous gas that is a minor component of the atmosphere. At the same time, in recent decades molecular hydrogen has been shown to have diverse biological effects. By the end of 2022, more than 2000 articles have been published in the field of hydrogen medicine, many of which are original studies. Despite the existence of several review articles on the biology of molecular hydrogen, many aspects of the research direction remain unsystematic. Therefore, the purpose of this review was to systematize ideas about the nature, characteristics, and mechanisms of the influence of molecular hydrogen on various types of cells, including stem cells. The historical aspects of the discovery of the biological activity of molecular hydrogen are presented. The ways of administering molecular hydrogen into the body are described. The molecular, cellular, tissue, and systemic effects of hydrogen are also reviewed. Specifically, the effect of hydrogen on various types of cells, including stem cells, is addressed. The existing literature indicates that the molecular and cellular effects of hydrogen qualify it to be a potentially effective agent in regenerative medicine.