bluuebunny/crossref_metadata_2025 huggingface dataset:
Neuroprotective and Anti-Inflammatory Effects of Low–Moderate Dose Ionizing Radiation in Models of Alzheimer’s Disease
Authors: Kim Sujin , Nam Yunkwon , Kim Chanyang , Lee Hyewon , Hong Seojin , Kim Hyeon Soo , Shin Soo Jung , Park Yong Ho , Mai Han Ngoc , Oh Sang-Muk , Kim Kyoung Soo , Yoo Doo-Han , Chung Weon Kuu , Chung Hyunju , Moon Minho
Published: May 2020

Alzheimer’s disease (AD) is the most common cause of dementia. The neuropathological features of AD include amyloid-β (Aβ) deposition and hyperphosphorylated tau accumulation. Although several clinical trials have been conducted to identify a cure for AD, no effective drug or treatment has been identified thus far. Recently, the potential use of non-pharmacological interventions to prevent or treat AD has gained attention. Low-dose ionizing radiation (LDIR) is a non-pharmacological intervention which is currently being evaluated in clinical trials for AD patients. However, the mechanisms underlying the therapeutic effects of LDIR therapy have not yet been established. In this study, we examined the effect of LDIR on Aβ accumulation and Aβ-mediated pathology. To investigate the short-term effects of low–moderate dose ionizing radiation (LMDIR), a total of 9 Gy (1.8 Gy per fraction for five times) were radiated to 4-month-old 5XFAD mice, an Aβ-overexpressing transgenic mouse model of AD, and then sacrificed at 4 days after last exposure to LMDIR. Comparing sham-exposed and LMDIR-exposed 5XFAD mice indicated that short-term exposure to LMDIR did not affect Aβ accumulation in the brain, but significantly ameliorated synaptic degeneration, neuronal loss, and neuroinflammation in the hippocampal formation and cerebral cortex. In addition, a direct neuroprotective effect was confirmed in SH-SY5Y neuronal cells treated with Aβ1–42 (2 μM) after single irradiation (1 Gy). In BV-2 microglial cells exposed to Aβ and/or LMDIR, LMDIR therapy significantly inhibited the production of pro-inflammatory molecules and activation of the nuclear factor-kappa B (NF-κB) pathway. These results indicate that LMDIR directly ameliorated neurodegeneration and neuroinflammation in vivo and in vitro. Collectively, our findings suggest that the therapeutic benefits of LMDIR in AD may be mediated by its neuroprotective and anti-inflammatory effects.
Molecular Basis of Bone Aging
Authors: Corrado Addolorata , Cici Daniela , Rotondo Cinzia , Maruotti Nicola , Cantatore Francesco Paolo
Published: May 2020

A decline in bone mass leading to an increased fracture risk is a common feature of age-related bone changes. The mechanisms underlying bone senescence are very complex and implicate systemic and local factors and are the result of the combination of several changes occurring at the cellular, tissue and structural levels; they include alterations of bone cell differentiation and activity, oxidative stress, genetic damage and the altered responses of bone cells to various biological signals and to mechanical loading. The molecular mechanisms responsible for these changes remain greatly unclear and many data derived from in vitro or animal studies appear to be conflicting and heterogeneous, probably due to the different experimental approaches; nevertheless, understanding the main physio-pathological processes that cause bone senescence is essential for the development of new potential therapeutic options for treating age-related bone loss. This article reviews the current knowledge concerning the molecular mechanisms underlying the pathogenesis of age-related bone changes.
Role of Extracellular Matrix in Gastrointestinal Cancer-Associated Angiogenesis
Authors: Andreuzzi Eva , Capuano Alessandra , Poletto Evelina , Pivetta Eliana , Fejza Albina , Favero Andrea , Doliana Roberto , Cannizzaro Renato , Spessotto Paola , Mongiat Maurizio
Published: May 2020

Gastrointestinal tumors are responsible for more cancer-related fatalities than any other type of tumors, and colorectal and gastric malignancies account for a large part of these diseases. Thus, there is an urgent need to develop new therapeutic approaches to improve the patients’ outcome and the tumor microenvironment is a promising arena for the development of such treatments. In fact, the nature of the microenvironment in the different gastrointestinal tracts may significantly influence not only tumor development but also the therapy response. In particular, an important microenvironmental component and a potential therapeutic target is the vasculature. In this context, the extracellular matrix is a key component exerting an active effect in all the hallmarks of cancer, including angiogenesis. Here, we summarized the current knowledge on the role of extracellular matrix in affecting endothelial cell function and intratumoral vascularization in the context of colorectal and gastric cancer. The extracellular matrix acts both directly on endothelial cells and indirectly through its remodeling and the consequent release of growth factors. We envision that a deeper understanding of the role of extracellular matrix and of its remodeling during cancer progression is of chief importance for the development of new, more efficacious, targeted therapies.
Mitophagy and Mitochondria Biogenesis Are Differentially Induced in Rat Skeletal Muscles during Immobilization and/or Remobilization
Authors: Deval Christiane , Calonne Julie , Coudy-Gandilhon Cécile , Vazeille Emilie , Bechet Daniel , Polge Cécile , Taillandier Daniel , Attaix Didier , Combaret Lydie
Published: May 2020

Mitochondria alterations are a classical feature of muscle immobilization, and autophagy is required for the elimination of deficient mitochondria (mitophagy) and the maintenance of muscle mass. We focused on the regulation of mitochondrial quality control during immobilization and remobilization in rat gastrocnemius (GA) and tibialis anterior (TA) muscles, which have very different atrophy and recovery kinetics. We studied mitochondrial biogenesis, dynamic, movement along microtubules, and addressing to autophagy. Our data indicated that mitochondria quality control adapted differently to immobilization and remobilization in GA and TA muscles. Data showed i) a disruption of mitochondria dynamic that occurred earlier in the immobilized TA, ii) an overriding role of mitophagy that involved Parkin-dependent and/or independent processes during immobilization in the GA and during remobilization in the TA, and iii) increased mitochondria biogenesis during remobilization in both muscles. This strongly emphasized the need to consider several muscle groups to study the mechanisms involved in muscle atrophy and their ability to recover, in order to provide broad and/or specific clues for the development of strategies to maintain muscle mass and improve the health and quality of life of patients.
Update on Congenital Myopathies in Adulthood
Authors: Papadimas George Konstantinos , Xirou Sophia , Kararizou Evangelia , Papadopoulos Constantinos
Published: May 2020

Congenital myopathies (CMs) constitute a group of heterogenous rare inherited muscle diseases with different incidences. They are traditionally grouped based on characteristic histopathological findings revealed on muscle biopsy. In recent decades, the ever-increasing application of modern genetic technologies has not just improved our understanding of their pathophysiology, but also expanded their phenotypic spectrum and contributed to a more genetically based approach for their classification. Later onset forms of CMs are increasingly recognised. They are often considered milder with slower progression, variable clinical presentations and different modes of inheritance. We reviewed the key features and genetic basis of late onset CMs with a special emphasis on those forms that may first manifest in adulthood.
Intrinsic Disorder in Tetratricopeptide Repeat Proteins
Authors: Van Bibber Nathan W. , Haerle Cornelia , Khalife Roy , Xue Bin , Uversky Vladimir N.
Published: May 2020

Among the realm of repeat containing proteins that commonly serve as “scaffolds” promoting protein-protein interactions, there is a family of proteins containing between 2 and 20 tetratricopeptide repeats (TPRs), which are functional motifs consisting of 34 amino acids. The most distinguishing feature of TPR domains is their ability to stack continuously one upon the other, with these stacked repeats being able to affect interaction with binding partners either sequentially or in combination. It is known that many repeat-containing proteins are characterized by high levels of intrinsic disorder, and that many protein tandem repeats can be intrinsically disordered. Furthermore, it seems that TPR-containing proteins share many characteristics with hybrid proteins containing ordered domains and intrinsically disordered protein regions. However, there has not been a systematic analysis of the intrinsic disorder status of TPR proteins. To fill this gap, we analyzed 166 human TPR proteins to determine the degree to which proteins containing TPR motifs are affected by intrinsic disorder. Our analysis revealed that these proteins are characterized by different levels of intrinsic disorder and contain functional disordered regions that are utilized for protein-protein interactions and often serve as targets of various posttranslational modifications.
Emerging Roles of Estrogen-Regulated Enhancer and Long Non-Coding RNAs
Authors: Sedano Melina , Harrison Alana , Zilaie Mina , Das Chandrima , Choudhari Ramesh , Ramos Enrique , Gadad Shrikanth
Published: May 2020

Genome-wide RNA sequencing has shown that only a small fraction of the human genome is transcribed into protein-coding mRNAs. While once thought to be “junk” DNA, recent findings indicate that the rest of the genome encodes many types of non-coding RNA molecules with a myriad of functions still being determined. Among the non-coding RNAs, long non-coding RNAs (lncRNA) and enhancer RNAs (eRNA) are found to be most copious. While their exact biological functions and mechanisms of action are currently unknown, technologies such as next-generation RNA sequencing (RNA-seq) and global nuclear run-on sequencing (GRO-seq) have begun deciphering their expression patterns and biological significance. In addition to their identification, it has been shown that the expression of long non-coding RNAs and enhancer RNAs can vary due to spatial, temporal, developmental, or hormonal variations. In this review, we explore newly reported information on estrogen-regulated eRNAs and lncRNAs and their associated biological functions to help outline their markedly prominent roles in estrogen-dependent signaling.
Unravelling the Potential of Graphene Quantum Dots in Biomedicine and Neuroscience
Authors: Perini Giordano , Palmieri Valentina , Ciasca Gabriele , De Spirito Marco , Papi Massimiliano
Published: May 2020

Quantum dots (QDs) are semiconducting nanoparticles that have been gaining ground in various applications, including the biomedical field, thanks to their unique optical properties. Recently, graphene quantum dots (GQDs) have earned attention in biomedicine and nanomedicine, thanks to their higher biocompatibility and low cytotoxicity compared to other QDs. GQDs share the optical properties of QD and have proven ability to cross the blood-brain barrier (BBB). For this reason, GQDs are now being employed to deepen our knowledge in neuroscience diagnostics and therapeutics. Their size and surface chemistry that ease the loading of chemotherapeutic drugs, makes them ideal drug delivery systems through the bloodstream, across the BBB, up to the brain. GQDs-based neuroimaging techniques and theranostic applications, such as photothermal and photodynamic therapy alone or in combination with chemotherapy, have been designed. In this review, optical properties and biocompatibility of GQDs will be described. Then, the ability of GQDs to overtake the BBB and reach the brain will be discussed. At last, applications of GQDs in bioimaging, photophysical therapies and drug delivery to the central nervous system will be considered, unraveling their potential in the neuroscientific field.
Studies on the Mechanisms of Anti-Inflammatory Activity of Heparin- and Hyaluronan-Containing Multilayer Coatings—Targeting NF-κB Signalling Pathway
Authors: Alkhoury Hala , Hautmann Adrian , Fuhrmann Bodo , Syrowatka Frank , Erdmann Frank , Zhou Guoying , Stojanović Sanja , Najman Stevo , Groth Thomas
Published: May 2020

The use of implants can be hampered by chronic inflammatory reactions, which may result in failure of the implanted device. To prevent such an outcome, the present study examines the anti-inflammatory properties of surface coatings made of either hyaluronic acid (HA) or heparin (Hep) in combination with chitosan (Chi) prepared as multilayers through the layer-by-layer (LbL) technique. The properties of glycosaminoglycan (GAG)-modified surfaces were characterized in terms of surface topography, thickness and wettability. Results showed a higher thickness and hydrophilicity after multilayer formation compared to poly (ethylene imine) control samples. Moreover, multilayers containing either HA or Hep dampened the inflammatory response visible by reduced adhesion, formation of multinucleated giant cells (MNGCs) and IL-1β release, which was studied using THP-1 derived macrophages. Furthermore, investigations regarding the mechanism of anti-inflammatory activity of GAG were focused on nuclear transcription factor-кB (NF-κB)-related signal transduction. Immunofluorescence staining of the p65 subunit of NF-κB and immunoblotting were performed that showed a significant decrease in NF-κB level in macrophages on GAG-based multilayers. Additionally, the association of FITC-labelled GAG was evaluated by confocal laser scanning microscopy and flow cytometry showing that macrophages were able to associate with and take up HA and Hep. Overall, the Hep-based multilayers demonstrated the most suppressive effect making this system most promising to control macrophage activation after implantation of medical devices. The results provide an insight on the anti-inflammatory effects of GAG not only based on their physicochemical properties, but also related to their mechanism of action toward NF-κB signal transduction.
ImergardTMWP: A Non-Chemical Alternative for an Indoor Residual Spray, Effective against Pyrethroid-Resistant Anopheles gambiae (s.l.) in Africa
Authors: Deguenon Jean M. , Azondekon Roseric , Agossa Fiacre R. , Padonou Gil G. , Anagonou Rodrigue , Ahoga Juniace , N’dombidje Boris , Akinro Bruno , Stewart David A. , Wang Bo , Gittins David , Tihomirov Larissa , Apperson Charles S. , McCord Marian G. , Akogbeto Martin C. , Roe R. Michael
Published: May 2020

Malaria is the deadliest mosquito-borne disease and kills predominantly people in sub-Saharan Africa (SSA). The now widespread mosquito resistance to pyrethroids, with rapidly growing resistance to other insecticide classes recommended by the World Health Organization (WHO), may overturn the successes gained in mosquito control in recent years. It is of utmost importance to search for new, inexpensive, and safe alternatives, with new modes of action, that might improve the efficacy of current insecticides. The efficacy of a novel mechanical insecticidal mineral derived from volcanic rock, ImergardTMWP, was investigated to determine its efficacy as a stand-alone residual wall spray and as a mixture with deltamethrin (K-Othrine® Polyzone) in experimental huts in Cove, Benin. The evaluation was conducted with susceptible (Kisumu) and wild-type Anopheles gambiae (s.l.). Deltamethrin applied alone demonstrated 40–45% mortality (at 72 h post-exposure) during the first four months, which declined to 25% at six months for wild An. gambiae from Cove. ImergardTMWP alone and mixed with deltamethrin, under the same assay conditions, produced 79–82% and 73–81% mortality, respectively, during the same six-month period. ImergardTMWP met the 80% WHO bio-efficacy threshold for residual activity for the first five months with 78% residual activity at six months. ImergardTMWP can be used as a mixture with chemical insecticides or as a stand-alone pesticide for mosquito control in Africa.
Phylogeny of Anopheles (Kerteszia) (Diptera: Culicidae) Using Mitochondrial Genes
Authors: Kirchgatter Karin , de Oliveira Guimarães Lilian , Hugo Yañez Trujillano Henrry , Rafael Arias Fernando , Cáceres Abraham , de Castro Duarte Ana , dos Santos Malafronte Rosely , Tubaki Rosa , Mureb Sallum Maria
Published: May 2020

Identification of mosquito species is necessary for determining the entomological components of malaria transmission, but it can be difficult in morphologically similar species. DNA sequences are largely used as an additional tool for species recognition, including those that belong to species complexes. Kerteszia mosquitoes are vectors of human and simian malaria in the Neotropical Region, but there are few DNA sequences of Kerteszia species in public databases. In order to provide relevant information about diversity and improve knowledge in taxonomy of Kerteszia species in Peru, we sequenced part of the mitochondrial genome, including the cytochrome c oxidase I (COI) barcode region. Phylogenetic analyses structured all species of mosquitoes collected in Peru into a single clade, separate from the Brazilian species. The Peruvian clade was composed of two lineages, encompassing sequences from Anopheles (Kerteszia) boliviensis and Anopheles (Kerteszia) pholidotus. An. pholidotus sequences were recorded for the first time in Peru, whereas An. boliviensis sequences were for the first time published in the GenBank database. Sequences generated from specimens morphologically identified as Anopheles (Kerteszia) cruzii clustered into three separate clades according to the collection localities of Serra do Mar, Serra da Mantiqueira, and Serra da Cantareira, confirming An. cruzii as a species complex, composed of at least three putative species.
Association between Periodontitis and High Blood Pressure: Results from the Study of Periodontal Health in Almada-Seixal (SoPHiAS)
Authors: Machado Vanessa , Aguilera Eva Muñoz , Botelho João , Hussain Syed Basit , Leira Yago , Proença Luís , D’Aiuto Francesco , Mendes José João
Published: May 2020

Periodontitis is a common chronic inflammatory disease which could have an important impact on blood pressure (BP). This study aimed to explore (a) the association between periodontal health and BP in a large representative cohort, (b) the predictive value of diagnosis of periodontitis in undiagnosed raised BP and (c) whether age is a mediator of this relationship. In total, 1057 randomly recruited individuals (mean age, 60.9 ± 16.3 years, 57.7% women) underwent periodontal clinical assessment and one-single BP measurement using an automated sphygmomanometer device. Logistic and linear regression models were used to estimate the odds of hypertension based on periodontitis case definitions. Mediation analysis was performed to understand the effect of age on the association of periodontitis with hypertension. Adjusted logistic model for gender, smoking habits and body mass index confirmed the association between high BP and periodontitis (OR = 2.31, 95%CI: 1.75–3.04, p < 0.001). Among 168 participants with undiagnosed high BP (15.9% of the study sample), 62.5% had periodontitis (n = 105). In this study, the association between periodontitis with both systolic blood pressure (SBP) (77.6%, p < 0.001) and diastolic blood pressure (DBP) (66.0%, p < 0.001) was mediated by age. Periodontitis is closely linked to BP in a representative Portuguese population.
Major Lipids, Apolipoproteins, and Alterations of Gut Microbiota
Authors: Yun Kyung Eun , Kim Jimin , Kim Mi-hyun , Park Eunkyo , Kim Hyung-Lae , Chang Yoosoo , Ryu Seungho , Kim Han-Na
Published: May 2020

The gut microbiota has been linked to blood lipids. However, the relationship between the gut microbiome and other lipid markers like apolipoproteins A1 (apoA1) and B (apoB) as well as classical lipid markers in Asians remain unclear. Here, we examined the associations between gut microbial diversity and taxonomic compositions with both apolipoproteins and lipid markers in a large number of Korean patients. The fecal 16S rRNA gene sequencing data from 1141 subjects were analyzed and subjects were categorized into control group (G0) or abnormal group (G1) according to blood lipid measurements. The microbial diversity and several taxa of the gut microbiota were significantly associated with triglyceride, apoA1, and apoB levels, but not with total cholesterol, low-density lipoprotein cholesterol, and high-density lipoprotein cholesterol levels. The alpha diversity of the gut microbiota was inversely associated with high triglyceride level. Interestingly, G1 of apoA1 showed increased microbial richness and distinct microbial community compared with G0 of apoA1. A high abundance of Fusobacteria and low abundance of Oscillospira were found in the hypertriglyceridemia group. In this large-scale study, we identified associations of gut microbiota with apolipoproteins and classical lipid markers, indicating that the gut microbiota may be an important target for regulating blood lipids.
Plasma Exchange or Immunoadsorption in Demyelinating Diseases: A Meta-Analysis
Authors: Lipphardt Mark , Wallbach Manuel , Koziolek Michael J.
Published: May 2020

Multiple sclerosis (MS) is an inflammatory disease mainly affecting the central nervous system. In MS, abnormal immune mechanisms induce acute inflammation, demyelination, axonal loss, and the formation of central nervous system plaques. The long-term treatment involves options to modify the disease progression, whereas the treatment for the acute relapse has its focus in the administration of high-dose intravenous methylprednisolone (up to 1000 mg daily) over a period of three to five days as a first step. If symptoms of the acute relapse persist, it is defined as glucocorticosteroid-unresponsive, and immunomodulation by apheresis is recommended. However, several national and international guidelines have no uniform recommendations on using plasma exchange (PE) nor immunoadsorption (IA) in this case. A systematic review and meta-analysis was conducted, including observational studies or randomized controlled trials that investigated the effect of PE or IA on different courses of MS and neuromyelitis optica (NMO). One thousand, three hundred and eighty-three patients were included in the evaluation. Therapy response in relapsing-remitting MS and clinically isolated syndrome was 76.6% (95%CI 63.7–89.8%) in PE- and 80.6% (95%CI 69.3–91.8%) in IA-treated patients. Based on the recent literature, PE and IA may be considered as equal treatment possibilities in patients suffering from acute, glucocorticosteroid-unresponsive MS relapses.
Propagation and Depolarization of a Short Pulse of Light in Sea Water
Authors: Gorodnichev Evgeniy E. , Kondratiev Kirill A. , Kuzovlev Alexandr I. , Rogozkin Dmitrii B.
Published: May 2020

We present the results of a theoretical study of underwater pulse propagation. The vector radiative transfer equation (VRTE) underlies our calculations of the main characteristics of the scattered light field in the pulse. Under the assumption of highly forward scattering in seawater, three separate equations for the basic modes are derived from the exact VRTE. These three equations are further solved both within the small-angle approximation and numerically. The equation for the intensity is analyzed for a power-law parametrization of the wings of the sea water phase function. The distribution of early arrival photons in the pulse, including the peak intensity, is calculated. Simple relations are also presented for the variance of the angular distribution of radiation, the effective duration of the signal and other parameters of the pulse. For linearly and circularly polarized pulses, the temporal profile of the degree of polarization is calculated for actual data on the scattering matrix elements. The degree of polarization is shown to be described by the self-similar dependence on some combination of the transport scattering coefficient, the temporal delay and the source-receiver distance. Our results are in agreement with experimental and Monte-Carlo simulation data. The conclusions of the paper offer a theoretical groundwork for application to underwater imaging, communication and remote sensing.
Development of Competitive–Cooperative Relationships among Mediterranean Cruise Ports since 2000
Authors: Lorenčič Vivien , Twrdy Elen , Batista Milan
Published: May 2020

Cruise shipping has been extremely popular in recent years, and one of the fastest-growing areas has been the Mediterranean. In the paper, we examine the evaluation of possible competitive–cooperative relationships among Mediterranean cruise ports for the period 2000–2017. To this end, we use three models: the dynamic shift-share model, the Lotka–Volterra model (LVM), and the logistic model (LM). The evaluation of basic market indices is included for completeness. The analysis shows that cruise traffic is in a saturation phase. The shift-share and LVM models reveal that interaction among ports is not significant for the large ports, but could be essential for the small ones.
Competition, Debt Maturity, and Adjustment Speed in China: A Dynamic Fractional Estimation Approach
Authors: Mirza Sultan Sikandar , Ahsan Tanveer , Safdar Raheel , Rehman Ajid Ur
Published: May 2020

The purpose of this study was to investigate the capital structure adjustment rate in different levels of product market competitions. We classified Chinese non-financial listed firms into highly, moderately, and less competitive firms and applied an unbiased dynamic panel fractional estimator on unbalanced panel data of 10,941 firm-year observations during the period of 1998 to 2015. We find that the adjustment rate of highly and less competitive firms towards long-term target capital structure is higher (28.2–29.1%) as compared to the adjustment rate towards short-term target capital structure (18.8–18.9%). On the other hand, the adjustment rate of moderately competitive firms towards long-term target capital structure is slower (22.3%) as compared to the adjustment rate towards short-term target capital structure (25.3%). Further, the adjustment rate of highly and less competitive firms differs significantly between long-term and short-term target capital structure, while the adjustment rate of moderately competitive firms remains steady. Highly competitive large firms follow the limited liability model to adjust their target capital structure and support trade-off theory, while both small and large firms follow the limited liability and predation models in moderately and less competitive environments, respectively.
Spatio-Temporal Coordination and Conflict of Production-Living-Ecology Land Functions in the Beijing-Tianjin-Hebei Region, China
Authors: Yu Zhuxiao , Xu Erqi , Zhang Hongqi , Shang Erping
Published: May 2020

Assessment of multiple land use functions promotes both utilization efficiency of land and regional coordination. Different personal and public products and services are offered by various land use types, meaning their functionality varies. Lack of judgment on temporal trends, turning points, or consideration of multi-source indicators like the ecological and air quality index leads to uncertainties in urban multifunctionality evaluation and functional orientation. In this study, the production-living-ecology land use function index system and evaluation process was improved using an entropy weight, triangle model, and coupling coordination degree. The production-living-ecology land use function (PLELUF) is defined from land use multi-functions. The Beijing-Tianjin-Hebei urban agglomeration was the representative area. The model was applied to quantify land use functions and measure spatio-temporal coordination and conflict from 1990 to 2015. Results found that the production and living functions displayed an overall upward trend and the growth rate of production function is larger, doubling from 1990 to 1995, while living function increases steadily. Ecology function remained steady from 1990 to 2000 but increased afterward. Land use function stage became balanced in ecology-living-production after 2005. No function-balanced cities existed in 1990; nine function-balanced cities were found in 2015. The coupling coordination degree increased from a slight conflict to a high coordination. Land use multi-functionality was high in the north and low in the south in 2015; Beijing had the most significant multifunctionality. This study can aid land use zoning and sustainable land management.
Old Ways, New Ways—Scaling Up from Customary Use of Plant Products to Commercial Harvest Taking a Multifunctional, Landscape Approach
Authors: Gorman Julian , Pearson Diane , Wurm Penelope
Published: May 2020

Globally, the agricultural sector is facing many challenges in response to climate change, unsustainable farming practices and human population growth. Despite advances in technology and innovation in agriculture, governments around the world are recognizing a need for transformative agricultural systems that offer solutions to the interrelated issues of food security, climate change, and conservation of environmental and cultural values. Approaches to production are needed that are holistic and multisectoral. In planning for future agricultural models, it is worth exploring indigenous agricultural heritage systems that have demonstrated success in community food security without major environmental impacts. We demonstrate how indigenous practices of customary harvest, operating in multifunctional landscapes, can be scaled up to service new markets while still maintaining natural and cultural values. We do this through a case analysis of the wild harvest of Kakadu plum fruit by Aboriginal people across the tropical savannas of northern Australia. We conclude that this system would ideally operate at a landscape scale to ensure sustainability of harvest, maintenance of important patterns and processes for landscape health, and incorporate cultural and livelihood objectives. Applied to a variety of similar native products, such a production system has potential to make a substantial contribution to niche areas of global food and livelihood security.
Health-Related Quality of Life and Frailty in Chronic Liver Diseases
Authors: Nishikawa Hiroki , Yoh Kazunori , Enomoto Hirayuki , Iwata Yoshinori , Sakai Yoshiyuki , Kishino Kyohei , Shimono Yoshihiro , Ikeda Naoto , Takashima Tomoyuki , Aizawa Nobuhiro , Takata Ryo , Hasegawa Kunihiro , Koriyama Takashi , Yuri Yukihisa , Nishimura Takashi , Nishiguchi Shuhei , Iijima Hiroko
Published: May 2020

We sought to examine the relationship between frailty and health-related quality of life as evaluated using the 36-item Short-Form Health Survey (SF-36) questionnaire in Japanese chronic liver disease (CLD) patients (n = 341, 122 liver cirrhosis cases, median age = 66 years). Frailty was defined as a clinical syndrome in which three or more of the following criteria were met (frailty score 3, 4, or 5): unintentional body weight loss, self-reported exhaustion, muscle weakness (grip strength: <26 kg in men and <18 kg in women), slow walking speed (<1.0 m/s), and low physical activity. Robust (frailty score 0), prefrail (frailty score 1 or 2), and frailty were found in 108 (31.7%), 187 (54.8%), and 46 (13.5%) patients, respectively. In all eight scales of the SF-36 (physical functioning, role physical, bodily pain, general health perception, vitality, social functioning, role emotion, and mental health), and the physical component summary score and mental component summary score, each score was well stratified according to the frailty status (all p < 0.0001). In the multivariate analysis, age (p = 0.0126), physical functioning (p = 0.0005), and vitality (p = 0.0246) were independent predictors linked to the presence of frailty. In conclusion, Japanese CLD patients with frailty displayed poorer conditions, both physically and mentally.
The Influence of Different Forms of Silver on Selected Pathogenic Bacteria
Authors: Buszewski Bogusław , Rogowska Agnieszka , Railean-Plugaru Viorica , Złoch Michał , Walczak-Skierska Justyna , Pomastowski Paweł
Published: May 2020

The application of silver nanoparticles as an antibacterial agent is becoming more common. Unfortunately, their effect on microorganisms is still not fully understood. Therefore, this paper attempts to investigate the influence of silver ions, biologically synthesized silver nanoparticles and nanoparticles functionalized with antibiotics on molecular bacteria profiles. The initial stage of research was aimed at the mechanism determination involved in antibiotics sorption onto nanoparticles’ surface. For this purpose, the kinetics study was performed. Next, the functionalized formulations were characterized by Fourier transform infrared spectroscopy (FT-IR), dynamic light scattering (DLS) and a zeta potential study. The results reveal that functionalization is a complex process, but does not significantly affect the stability of biocolloids. Furthermore, the antimicrobial assays, in most cases, have shown no increases in antibacterial activity after nanoparticle functionalization, which suggests that the functionalization process does not always generate the improved antimicrobial effect. Finally, the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) technique was employed to characterize the changes in the molecular profile of bacteria treated with various antibacterial agents. The recorded spectra proved many differences in bacterial lipids and proteins profiles compared to untreated cells. In addition, the statistical analysis of recorded spectra revealed the strain-dependent nature of stress factors on the molecular profile of microorganisms.
Current Status and Prospects of Polymer Powder 3D Printing Technologies
Authors: Wang Yue , Xu Zhiyao , Wu Dingdi , Bai Jiaming
Published: May 2020

3D printing technology, which greatly simplifies the manufacturing of complex parts by a two-dimensional layer-upon-layer process, has flourished in recent years. As one of the most advanced technology, polymer powder 3D printing has many advantages such as high materials utilization rate, free of support structure, great design freedom, and large available materials, which has shown great potential and prospects in various industry applications. With the launch of the Multi jet Fusion system from HP, polymer powder 3D printing has been attracting more attention from industries and researchers. In this work, a comprehensive review of the main polymer powder-based 3D printing methods including binder jetting, selective laser sintering, high-speed sintering were carried out. Their forming mechanism, advantages and drawbacks, materials, and developments were presented, compared, and discussed respectively. In addition, this paper also gives suggestions on the process selection by comparing typical equipment parameters and features of each technology.
Numerical Characterization for Electrical Conductivity of Two-Dimensional Nanocomposite Systems with Conducting Fiber Fillers
Authors: Lee Jungmin , Yun Yesol , Lee Sang Hyun , Hwang Jinyoung
Published: May 2020

Hybrid nanotube composite systems with two different types of fillers attract considerable attention in several applications. The incorporation of secondary fillers exhibits conflicting behaviors of the electrical conductivity, which either increases or decreases according to the dimension of secondary fillers. This paper addresses quantitative models to predict the electrical performance in the configuration of two dimensional systems with one-dimensional secondary fillers. To characterize these properties, Monte Carlo simulations are conducted for percolating networks with a realistic model with the consideration of the resistance of conducting NWs, which conventional computational approaches mostly lack from the common assumption of zero-resistance or perfect conducting NWs. The simulation results with nonperfect conductor NWs are compared with the previous results of perfect conductors. The variation of the electrical conductivity reduces with the consideration of the resistance as compared to the cases with perfect conducting fillers, where the overall electrical conductivity solely originates from the contact resistance caused by tunneling effects between NWs. In addition, it is observed that the resistance associated with the case of invariant conductivity with respect to the dimension of the secondary fillers increases, resulting in the need for secondary fillers with the increased scale to achieve the same electrical performance. The results offer useful design guidelines for the use of a two-dimensional percolation network for flexible conducting electrodes.
Properties of Fibrous Concrete Made with Plastic Optical Fibers from E-Waste
Authors: Suchorab Zbigniew , Franus Małgorzata , Barnat-Hunek Danuta
Published: May 2020

This article presents research results relating to the potential for waste utilization in the form of polymer optical fiber (POF) scraps. This material is difficult to recycle due to its diverse construction. Three different volumes of POF were used in concrete in these tests: 1%, 2%, and 3%. The experimental studies investigated the basic properties of the concrete, the elastic and dynamic moduli, as well as deformation and deflection of reinforced beams. The microstructures, including the interfacial transition zones (ITZs), were recorded and analyzed using a scanning electron microscope. It was observed that 180 freezing–thawing cycles reduced the concrete frost resistance containing 3% POFs by half compared to the control concrete. The resistance to salt crystallization of this concrete decreased by about 55%. POFs have significant effects on the splitting tensile and flexural strengths compared to the compressive strength. The control beams were destroyed during the four-point static bending tests at half the force applied to the beams that were reinforced with POFs.
Capacitance Enhancement by Incorporation of Functionalised Carbon Nanotubes into Poly(3,4-Ethylenedioxythiophene)/Graphene Oxide Composites
Authors: Cymann Anita , Sawczak Mirosław , Ryl Jacek , Klugmann-Radziemska Ewa , Wilamowska-Zawłocka Monika
Published: May 2020

This paper reports on the role of oxidised carbon nanotubes (oxMWCNTs) present in poly-3,4-ethylenedioxytiophene (PEDOT)/graphene oxide (GOx) composite. The final ternary composites (pEDOT/GOx/oxMWCNTs) are synthesised by an electrodeposition process from the suspension-containing monomer, oxidised carbon nanotubes and graphene oxide. Dissociated functional groups on the surface of graphene oxide play a role of counter-ions for the polymer chains. Detailed physicochemical and electrochemical characterisation of the ternary composites is presented in the paper. The results prove that the presence of oxMWCNTs in the ternary composites doubles the capacitance values compared to the binary ones (450 vs. 270 F cm−3 for PEDOT/GOx/oxMWCNTs and PEDOT/GOx, respectively). The amount of carbon nanotubes in the synthesis solution is crucial for physicochemical properties of the composites, their adhesion to the electrode substrate and the electrochemical performance.
Functionalized Electrospun Nanofibers as a Versatile Platform for Colorimetric Detection of Heavy Metal Ions in Water: A Review
Authors: Balusamy Brabu , Senthamizhan Anitha , Uyar Tamer
Published: May 2020

The increasing heavy metal pollution in the aquatic ecosystem mainly driven by industrial activities has raised severe concerns over human and environmental health that apparently necessitate the design and development of ideal strategies for the effective monitoring of heavy metals. In this regard, colorimetric detection provides excellent opportunities for the easy monitoring of heavy metal ions, and especially, corresponding solid-state sensors enable potential opportunities for their applicability in real-world monitoring. As a result of the significant interest originating from their simplicity, exceptional characteristics, and applicability, the electrospun nanofiber-based colorimetric detection of heavy metal ions has undergone radical developments in the recent decade. This review illustrates the range of various approaches and functional molecules employed in the fabrication of electrospun nanofibers intended for the colorimetric detection of various metal ions in water. We highlight relevant investigations on the fabrication of functionalized electrospun nanofibers encompassing different approaches and functional molecules along with their sensing performance. Furthermore, we discuss upcoming prospectus and future opportunities in the exploration of designing electrospun nanofiber-based colorimetric sensors for real-world applications.
Progressive Failure Analysis of Laminates with Embedded Wrinkle Defects Based on an Elastoplastic Damage Model
Authors: Ning Zhi Hua , Huo Guan Liang , Liu Ren Huai , Wu Wei Lin , Xie Jia Ming
Published: May 2020

Out-of-plane wrinkling has a significant influence on the mechanical performance of composite laminates. Numerical simulations were conducted to investigate the progressive failure behavior of fiber-reinforced composite laminates with out-of-plane wrinkle defects subjected to axial compression. To describe the material degradation, a three-dimensional elastoplastic damage model with four damage modes (i.e., fiber tensile failure, matrix failure, fiber kinking/splitting, and delamination) was developed based on the LaRC05 criterion. To improve the computational efficiency in searching for the fracture angle in the matrix failure analysis, a high-efficiency and robust modified algorithm that combines the golden section search method with an inverse interpolation based on an existing study is proposed. The elastoplastic damage model was implemented in the finite-element code Abaqus using a user-defined material subroutine in Abaqus/Explicit. The model was applied to the progressive failure analysis of IM7/8552 composite laminates with out-of-plane wrinkles subjected to axial compressive loading. The numerical results showed that the compressive strength prediction obtained by the elastoplastic damage model is more accurate than that derived with an elastic damage model. The present model can describe the nonlinearity of the laminate during the damage evolution and determine the correct damage locations, which are in good agreement with experimental observations. Furthermore, it was discovered that the plasticity effects should not be neglected in laminates with low wrinkle levels.
Local Motion Planner for Autonomous Navigation in Vineyards with a RGB-D Camera-Based Algorithm and Deep Learning Synergy
Authors: Aghi Diego , Mazzia Vittorio , Chiaberge Marcello
Published: May 2020

With the advent of agriculture 3.0 and 4.0, in view of efficient and sustainable use of resources, researchers are increasingly focusing on the development of innovative smart farming and precision agriculture technologies by introducing automation and robotics into the agricultural processes. Autonomous agricultural field machines have been gaining significant attention from farmers and industries to reduce costs, human workload, and required resources. Nevertheless, achieving sufficient autonomous navigation capabilities requires the simultaneous cooperation of different processes; localization, mapping, and path planning are just some of the steps that aim at providing to the machine the right set of skills to operate in semi-structured and unstructured environments. In this context, this study presents a low-cost, power-efficient local motion planner for autonomous navigation in vineyards based only on an RGB-D camera, low range hardware, and a dual layer control algorithm. The first algorithm makes use of the disparity map and its depth representation to generate a proportional control for the robotic platform. Concurrently, a second back-up algorithm, based on representations learning and resilient to illumination variations, can take control of the machine in case of a momentaneous failure of the first block generating high-level motion primitives. Moreover, due to the double nature of the system, after initial training of the deep learning model with an initial dataset, the strict synergy between the two algorithms opens the possibility of exploiting new automatically labeled data, coming from the field, to extend the existing model’s knowledge. The machine learning algorithm has been trained and tested, using transfer learning, with acquired images during different field surveys in the North region of Italy and then optimized for on-device inference with model pruning and quantization. Finally, the overall system has been validated with a customized robot platform in the appropriate environment.
Synthesis and Characterization of Novel Nanoporous Gl-POSS-Branched Polymeric Gas Separation Membranes
Authors: Zaripov Ilnaz , Davletbaeva Ilsiya , Faizulina Zulfiya , Davletbaev Ruslan , Gubaidullin Aidar , Atlaskin Artem , Vorotyntsev Ilya
Published: May 2020

Novel nanoporous Gl-POSS-branched polymers based on the macroinitiator of anionic nature, 2,4-toluene diisocyanate, and octaglycidyl polyhedral oligomeric silsesquioxane (Gl-POSS) were obtained as gas separation membranes. The synthesis of polymers was carried out using various loads of Gl-POSS. It was found that the main reaction proceeding with 2,4-toluene diisocyanate is the polyaddition, accompanied by the isocyanate groups opening of the carbonyl part. This unusual opening of isocyanate groups leads to the formation of coplanar acetal nature polyisocyanates (O-polyisocyanate). The terminal O-polyisocyanate links initiate the subsequent opening of the epoxide rings in Gl-POSS. As a result, Gl-POSS serves as a hard and bulky branching agent and creates the specific framing supramolecular structure, which leads to the formation of nanopores in the polymer, where the flexible polyether components are located inside the cavities. Thermal, mechanical, physical, and chemical properties of the obtained polymers were studied at various Gl-POSS contents in the polymer matrix. It was found that these polymers show high selectivity of gas transport properties for pure ammonia relative to nitrogen and hydrogen at ambient temperature. Measurements showed that the gas permeability coefficients and the values of ideal selectivity were in a non-additive dependence to the Gl-POSS content.
Numerical Analysis of Stress Distribution in Offshore Wind Turbine M72 Bolted Connections
Authors: Redondo Raquel , Mehmanparast Ali
Published: May 2020

The use of bolted joints to connect the transition piece and monopile is nowadays widely applied in the offshore wind industry. Traditionally, grouted connections were used in the early generation of offshore wind turbines, but the experienced failures in such connections led to an increased tendency towards bolted flange connections to join the transition piece and monopile in the new generation of offshore wind turbines. The bolts used for this purpose have high strength and huge sizes, and are subjected to a preload that is applied during the tightening process. The present study is focused on the analysis of preload effects on stress distribution in M72 bolted connections by considering different friction coefficients between the bolt and nut threads. The bolt is considered to be made of grade 10.9 steel, whereas the nut is assumed to be made of grade 8.8 steel, which is a softer material. Using the finite element commercial software package Abaqus, numerical models were developed and analysed to establish trends for stress distribution and plastic strains during the bolt tightening process, and to quantify stress concentration factors in individual engaged threads.