bluuebunny/crossref_metadata_2025 huggingface dataset:
Numerical Simulation and Modeling of Mechano–Electro–Thermal Behavior of Electrical Contact Using COMSOL Multiphysics
Authors: Andras Andrei , Popescu Florin Dumitru , Radu Sorin Mihai , Pasculescu Dragos , Brinas Ildiko , Radu Mirela Ancuta , Peagu Daniela (Furdui)
Published: May 2024

Electrical contacts are important circuit components with diverse industrial applications, and their failure can lead to multiple unwanted effects. Hence, the behavior of electrical contacts is a widely studied topic in the scientific literature based on various approaches, tools, and techniques. The present study proposes a new approach to numerical modeling and simulation based on the Holm contact theory, aiming to study the dependence between the electric potential and the temperature within an electrical contact. Structured in five sections, the research was conducted using COMSOL Multiphysics software (version 5.3) and its solid-state mechanics, electric current, and heat transfer modules in order to highlight contact behavior from mechanical, electrical and thermal points of view: the von Mises stress, contact force, electric field amplitude, variation of the electrical potential along the current path, temperature gradient, and dependence of temperature along the contact elements edges were obtained by simulation, and are graphically represented. The results show that the temperature increase follows a parabolic curve, and that for values higher than 4 mV of voltage drop, the temperature of the contact increases to 79.25 degrees (and up to 123.81 degrees for 5 mV) over the ambient temperature, thus the integrity of insulation can be compromised. These values are close (10–12%) to the analytically calculated ones, and also in line with research assessed in the literature review.
The Effect of Apple Vinegar Addition on the Quality and Shelf Life of Cooked Sausage during Chilling Storage
Authors: Okoń Anna , Zielińska Dorota , Szymański Piotr , Łepecka Anna , Siekierko Urszula , Neffe-Skocińska Katarzyna , Trząskowska Monika , Kajak-Siemaszko Katarzyna , Sionek Barbara , Karbowiak Marcelina , Kołożyn-Krajewska Danuta , Dolatowski Zbigniew J.
Published: May 2024

As more and more consumers are becoming conscious of the safety and taste of meat products, the use of natural additives and innovative processing techniques has gained significant attention. Naturally fermented fruit vinegar is rich in organic acids and antioxidant phenolic compounds. In addition, it contains amino acids, minerals, vitamins, and provitamin beta-carotene, and the presence of acetic acid bacteria may have a positive effect on consumer health. The study aimed to assess the impact of different concentrations of apple vinegar addition on the quality of cooked sausage, focusing on physicochemical parameters, including fatty acid profile and oxidative stability, as well as microbiological quality and sensory changes after production and during chilling storage. Four variants of sausage were prepared: C—sausage without apple vinegar; V1—sausage with 1% of apple vinegar; V3—sausage with 3% of apple vinegar; and V5—sausage with 5% of apple vinegar. All of the tests were carried out after production, as well as after 7 and 14 days of refrigeration storage. The addition of apple vinegar decreased the pH value and increased the oxidation-reduction potential and lipid oxidation in the samples V1, V3, and V5. The sausage with the 5% addition of apple vinegar (V5) was characterized by significantly more intensive brightness (parameter L* = 54.67) in comparison to the C sample (parameter L* = 52.78). The sausages that were tested showed good microbiological quality concerning the total number of microorganisms, lactic acid bacteria, and the absence of pathogenic bacteria. The addition of apple vinegar contributed to the reduction in the intensity of the cured meat flavor and the fatty flavor. Therefore, according to the results presented in this work, it can be concluded that 3% of vinegar is the optimal addition, which may be used in the next step of investigation, taking into account color formation abilities as well as microbiological quality and lipid oxidation processes.
Enhancement of the Vegetation Carbon Uptake by the Synergistic Approach to Air Pollution Control and Carbon Neutrality in China
Authors: Qin Xiao , Shi Guangming , Yang Fumo
Published: May 2024

Carbon sinks provided by land ecosystems play a crucial role in achieving carbon neutrality. However, the future potential of carbon sequestration remains highly uncertain. The impact of pollutant emission reduction (PER) introduced by the proposed synergistic approach to air pollution control and carbon neutrality on carbon sinks in China has not yet been fully evaluated. In this study, we analyzed the effects of regional carbon-neutral PER policies, global climate change, and their coupled effects on China’s terrestrial gross primary productivity (GPP) by conducting numerical experiments using the weather research and forecasting model coupled with chemistry (WRF-Chem) and the moderate resolution imaging spectroradiometer photosynthesis algorithm (MODIS-PSN). We found that carbon-neutral PER policies could promote GPP growth in most regions of China in 2060, particularly during April and October, resulting in a total increase of at least 21.84 TgC compared to that in 2016, which offset the adverse effects of global climate change up to fourfold. The aerosol radiative effects drive GPP growth under carbon-neutral PER policies, primarily through an increase in daily minimum temperature during winter and an increase in shortwave radiation during other seasons. Our research highlights that reducing pollutant emissions enhances future potential for carbon sequestration, revealing positive feedback towards achieving the target of carbon neutrality.
Posterior Semicircular Canal Plugging Relieves Tumarkin’s Crisis in Ménière’s Disease Patients
Authors: Comacchio Francesco , Bordin Anna , Di Pasquale Fiasca Valerio Maria , Bellemo Barbara , Magnavita Paola , Fasanaro Elena , Poletto Elisabetta
Published: May 2024

(1) Background: Patients affected by Ménière’s disease can experience Tumarkin’s syndrome, which is characterized by postural instability, gait abnormalities, and, occasionally, an abrupt loss of balance known as vestibular drop attack or Tumarkin’s crisis. In this study, semicircular canal plugging is proposed as the definitive treatment for this condition. The outcomes of this type of surgery are discussed. (2) Methods: A total of 9 patients with a confirmed diagnosis of Ménière disease suffering from Tumarkin crisis underwent posterior semicircular canal plugging. These patients were assessed with Video Head Impulse Tests, vestibular evoked myogenic potentials, and Pure Tone Audiometry preoperatively and postoperatively. (3) Results: VHIT showed a postoperative decrease in PSC gain median (Preop. 0.86 and postop. 0.52; p < 0.009). No statistically significant differences were described for the anterior semicircular canal and the lateral semicircular canal. No patient experienced new Tumarkin crisis after the surgical treatment. (4) Conclusions: Our ten years of experience with posterior semicircular canal plugging in Ménière disease patients with Tumarkin’s syndrome has shown that this type of surgical procedure is successful in controlling Tumarkin’s crisis, with high patient satisfaction and little worsening in hearing level.
Study of the Six-Compartment Nonlinear COVID-19 Model with the Homotopy Perturbation Method
Authors: Rafiullah Muhammad , Asif Muhammad , Jabeen Dure , Ibrahim Mahmoud A.
Published: May 2024

The current study aims to utilize the homotopy perturbation method (HPM) to solve nonlinear dynamical models, with a particular focus on models related to predicting and controlling pandemics, such as the SIR model. Specifically, we apply this method to solve a six-compartment model for the novel coronavirus (COVID-19), which includes susceptible, exposed, asymptomatic infected, symptomatic infected, and recovered individuals, and the concentration of COVID-19 in the environment is indicated by S(t), E(t), A(t), I(t), R(t), and B(t), respectively. We present the series solution of this model by varying the controlling parameters and representing them graphically. Additionally, we verify the accuracy of the series solution (up to the (n−1)th-degree polynomial) that satisfies both the initial conditions and the model, with all coefficients correct at 18 decimal places. Furthermore, we have compared our results with the Runge–Kutta fourth-order method. Based on our findings, we conclude that the homotopy perturbation method is a promising approach to solve nonlinear dynamical models, particularly those associated with pandemics. This method provides valuable insight into how the control of various parameters can affect the model. We suggest that future studies can expand on our work by exploring additional models and assessing the applicability of other analytical methods.
Canonical Metrics on Twisted Quiver Bundles over a Class of Non-Compact Gauduchon Manifold
Authors: Cai Shi-Fan , Chaubey Sudhakar Kumar , Xu Xin , Zhang Pan , Zhang Zhi-Heng
Published: May 2024

The aim of this paper is to prove a theorem for holomorphic twisted quiver bundles over a special non-compact Gauduchon manifold, connecting the existence of (σ,τ)-Hermite–Yang–Mills metric in differential geometry and the analytic (σ,τ)-stability in algebraic geometry. The proof of the theorem relies on the flow method and the Uhlenbeck–Yau’s continuity method.
Females Present Different Single-Leg Squat Kinematics and Muscle Activation Strategies than Males Even after Hip Abductor Fatigue
Authors: Gaviraghi Pablo , Chaida Sonda Francesca , Fernandes Frigotto Michele , Molinari Talita , Pizarro Chaffe Luiza , Flor José Luis , Rabello Rodrigo , Rodrigues Rodrigo
Published: May 2024

Background: Despite the potential connection between hip and knee muscle control, there is limited research on the effects of hip abductor fatigue on the hip and knee neuromuscular responses in both males and females. This study aimed to investigate the influence of sex on the hip and knee frontal plane kinematics and the EMG responses of the hip abductors and knee extensor muscles during the single-leg squat before and after hip abductor fatigue. Methods: A total of 30 participants (males, n = 15; females, n = 15) performed single-leg squats before and immediately after a hip abductor fatigue protocol (10° hip abduction position while bearing a 20% load of their estimated 1RM until exhaustion). The frontal plane kinematics (hip adduction and knee frontal plane projection angle) and EMG parameters (amplitude and median frequency) of the gluteus medius (GMed), tensor fascia latae (TFL), vastus lateralis (VL) and vastus medialis (VM) were measured during the single-leg squat. Results: We did not find any effects of hip abductor fatigue or interaction between fatigue and sex on the evaluated parameters (p > 0.05). However, compared to males, females had greater values for the hip and knee frontal plane kinematics (p = 0.030), GMed EMG amplitude (+10.2%, p = 0.012) and median frequency (+10.3%; p = 0.042) and lower VL median frequency (−9.80%; p = 0.007). Conclusions: These findings establish sex-related differences in the kinematics and hip and knee EMG parameters during the single-leg squat, which were not influenced by the hip abductor fatigue protocol.
Targeted Formation of Biofilms on the Surface of Graphite Electrodes as an Effective Approach to the Development of Biosensors for Early Warning Systems
Authors: Kharkova Anna , Perchikov Roman , Kurbanalieva Saniyat , Osina Kristina , Popova Nadezhda , Machulin Andrey , Kamanina Olga , Saverina Evgeniya , Saltanov Ivan , Melenkov Sergey , Butusov Denis , Arlyapov Vyacheslav
Published: May 2024

Biofilms based on bacteria Pseudomonas veronii (Ps. veronii) and Escherichia coli (E. coli) and yeast Saccharomyces cerevisiae (S. cerevisiae) were used for novel biosensor creation for rapid biochemical oxygen demand (BOD) monitoring. Based on the electrochemical measurement results, it was shown that the endogenous mediator in the matrix of E. coli and Ps. veronii biofilms and ferrocene form a two-mediator system that improves electron transport in the system. Biofilms based on Ps. veronii and E. coli had a high biotechnological potential for BOD assessment; bioreceptors based on such biofilms had high sensitivity (the lower limits of detectable BOD5 concentrations were 0.61 (Ps. veronii) and 0.87 (E. coli) mg/dm3) and high efficiency of analysis (a measurement time 5–10 min). The maximum biosensor response based on bacterial biofilms has been observed in the pH range of 6.6–7.2. The greatest protective effect was found for biofilms based on E. coli, which has high long-term stability (151 days for Ps. veronii and 163 days for E. coli). The results of the BOD5 analysis of water samples obtained using the developed biosensors had a high correlation with the results of the standard 5-day method (R2 = 0.9820, number of tested samples is 10 for Ps. veronii, and R2 = 0.9862, number of tested samples is 10 for E. coli). Thus, biosensors based on Ps. veronii biofilms and E. coli biofilms could be a novel analytical system to give early warnings of pollution.
Thermal Environment Analysis and Optimization for Large Space Buildings with Radiant Cooling Floors: A Case Study of Xianyang International Airport
Authors: Hu Rong , Wang Haolin , Liang Junqi , Li Xiaoping , Zheng Wenheng , Liu Gang
Published: May 2024

Radiant cooling floors combined with ventilation systems have been widely applied in large space buildings. However, there has been a lack of research on system control strategies for their adaptation to weather changes. This study aimed to find control strategies for radiant cooling floors combined with displacement ventilation systems used in large space buildings in order to achieve energy conservation and environmental improvement. Supply air temperature and cooling surface temperature were determined to be the control variables. It was found that cooling capacity of the combined system and the comfort index, PMV (predicted mean vote), were linear in relation to the supply air temperature and cooling surface temperature. The linear equations regarding cooling capacity and PMV were established separately using environment data, and then the optimal region was determined. A case study on Terminal 3 of Xi’an Xianyang International Airport was conducted. The thermal environment was investigated through on-site measurements, questionnaires, and numerical simulations with CFD (computational fluid dynamics). It was found that supply air temperature and cooling surface temperature had a significant impact on PMV, and less impact on the cooling capacity. Therefore, it was determined that the supply air temperature should be altered first when the indoor temperature exceeds the upper limit, and then the cooling surface temperature should be changed if the indoor environment continues to overheat with the supply air temperature set to 18 °C. Thus, the supply air temperature was kept at 18 °C, and the floor surface temperature was set to be 22 °C on a high-temperature day. The average PMV was 0.87, and the cooling capacity of the combined system was 200 W/(m2·K), according to the CFD simulation. In addition, the surface heat transfer coefficient of the cooling floor was found to be 10.26 W/(m2·K). This research provides important references for the design and operational management of radiant cooling floors in large space buildings.
Prediction of Mismatch Repair Status in Endometrial Cancer from Histological Slide Images Using Various Deep Learning-Based Algorithms
Authors: Umemoto Mina , Mariya Tasuku , Nambu Yuta , Nagata Mai , Horimai Toshihiro , Sugita Shintaro , Kanaseki Takayuki , Takenaka Yuka , Shinkai Shota , Matsuura Motoki , Iwasaki Masahiro , Hirohashi Yoshihiko , Hasegawa Tadashi , Torigoe Toshihiko , Fujino Yuichi , Saito Tsuyoshi
Published: May 2024

The application of deep learning algorithms to predict the molecular profiles of various cancers from digital images of hematoxylin and eosin (H&E)-stained slides has been reported in recent years, mainly for gastric and colon cancers. In this study, we investigated the potential use of H&E-stained endometrial cancer slide images to predict the associated mismatch repair (MMR) status. H&E-stained slide images were collected from 127 cases of the primary lesion of endometrial cancer. After digitization using a Nanozoomer virtual slide scanner (Hamamatsu Photonics), we segmented the scanned images into 5397 tiles of 512 × 512 pixels. The MMR proteins (PMS2, MSH6) were immunohistochemically stained, classified into MMR proficient/deficient, and annotated for each case and tile. We trained several neural networks, including convolutional and attention-based networks, using tiles annotated with the MMR status. Among the tested networks, ResNet50 exhibited the highest area under the receiver operating characteristic curve (AUROC) of 0.91 for predicting the MMR status. The constructed prediction algorithm may be applicable to other molecular profiles and useful for pre-screening before implementing other, more costly genetic profiling tests.
Highly Efficient PtSn/Al2O3 and PtSnZnCa/Al2O3 Catalysts for Ethane Dehydrogenation: Influence of Catalyst Pretreatment Atmosphere
Authors: Podila Seetharamulu , Al-Zahrani Abdulrahim A. , Daous Muhammad A. , Alhumade Hesham
Published: May 2024

Increased demand for ethylene has motivated direct ethane dehydrogenation over Pt-based catalysts. PtSn/γ-Al2O3 and PtSnZnCa/γ-Al2O3 catalysts were investigated with the aim of understanding the effect of the pretreatment environment on the state of dispersed Pt for ethane dehydrogenation. The catalysts were prepared by the impregnation method and pretreated in different environments like static air (SA), flowing air (FA), and nitrogen (N2) atmospheres. A comprehensive characterization of the catalysts was performed using Brunauer–Emmett–Teller (BET), X-ray diffraction (XRD), Temperature-Programmed Reduction (TPR), NH3 Temperature-Programmed Desorption (NH3-TPD), X-ray photoelectron spectroscopy (XPS), and Transmission Electron Microscopy (TEM) techniques. The results reveal that the PtSn on Al2O3 catalyst pretreated in the static air environment (PtSn-SA) exhibits 21% ethylene yield with 95% selectivity at 625 °C. XPS analysis found more platinum and tin on the catalyst surface after static air treatment. The overall acidity of the catalysts decreased after thermal treatment in static air. Elemental mapping demonstrated that Pt agglomeration was pronounced in catalysts calcined under flowing air and nitrogen. These factors are responsible for the enhanced activity of the PtSn-SA catalyst compared to the other catalysts. The addition of Zn and Ca to the PtSn catalysts increases the yield of the catalyst calcined in static air (PtSnZnCa-SA). The PtSnZnCa-SA catalyst showed the highest ethylene yield of 27% with 99% selectivity and highly stable activity at 625 °C for 10 h.
A Systematic Review of Using Deep Learning in Aphasia: Challenges and Future Directions
Authors: Wang Yin , Cheng Weibin , Sufi Fahim , Fang Qiang , Mahmoud Seedahmed S.
Published: May 2024

In this systematic literature review, the intersection of deep learning applications within the aphasia domain is meticulously explored, acknowledging the condition’s complex nature and the nuanced challenges it presents for language comprehension and expression. By harnessing data from primary databases and employing advanced query methodologies, this study synthesizes findings from 28 relevant documents, unveiling a landscape marked by significant advancements and persistent challenges. Through a methodological lens grounded in the PRISMA framework (Version 2020) and Machine Learning-driven tools like VosViewer (Version 1.6.20) and Litmaps (Free Version), the research delineates the high variability in speech patterns, the intricacies of speech recognition, and the hurdles posed by limited and diverse datasets as core obstacles. Innovative solutions such as specialized deep learning models, data augmentation strategies, and the pivotal role of interdisciplinary collaboration in dataset annotation emerge as vital contributions to this field. The analysis culminates in identifying theoretical and practical pathways for surmounting these barriers, highlighting the potential of deep learning technologies to revolutionize aphasia assessment and treatment. This review not only consolidates current knowledge but also charts a course for future research, emphasizing the need for comprehensive datasets, model optimization, and integration into clinical workflows to enhance patient care. Ultimately, this work underscores the transformative power of deep learning in advancing aphasia diagnosis, treatment, and support, heralding a new era of innovation and interdisciplinary collaboration in addressing this challenging disorder.
Genetic Diversity and Population Structure among Arabian Horse Genealogical Lineages in Bulgaria
Authors: Hristov Peter , Radoslavov Georgi , Mehandjyiski Ivan , Salkova Delka , Yordanov Georgi
Published: May 2024

The present research aimed to characterize the genetic diversity and relationships among extant Arabian horse sire lines in Bulgaria, using 15 equine microsatellite markers. The evaluation included 537 Arabian horses representing nine sire lines (SAKLAWI I, LATIF, SEANDERICH, IBRAHIM, SHABAB, DJEBEL MOUSA, KUHAILAN AFAS, BAIRACTAR, and SARHAN). The obtained results indicated that within these lines, the mean number of alleles ranged from 4.15 in SARHAN to 5.54 in SAKLAWI I and LATIF. The mean expected heterozygosity (He) ranged from 0.54 in the SEANDERICH line to 0.67 in SAKLAWI I. The inbreeding coefficient for the entire Arabian populations was rather low: FIS = −0.109, fluctuating from −0.204 in SHABAB to −0.041 in SAKLAWI I. The mean genetic differentiation, FST, was 0.096, demonstrating that nearly 90% of the total genetic variation was due to genetic differentiation within each population. STRUCTURE analysis indicated a genetic similarity between SHABAB and LATIF, between IBRAHIM and KUHAILAN AFAS, as well as between SAKLAWI I, SEANDERICH, and BAIRACTAR. This study of the genetic diversity of Arabian sire lines in Bulgaria can assist in developing a national strategy for the exclusion of non-purebred animals from breeding programs in order to preserve the genetic profile of the original Arabian lines.
Nice for Whom? A Dangerous, Not-So-Nice, Critical Race Love Letter
Authors: Reyes G. T.
Published: May 2024

In this article, I critically analyze and respond to empirical data in the form of racialized discourse—specifically, racist messages sent directly to me as a result of my previously published article entitled, “A Love Letter to Educational Leaders of Color: CREWing UP with Critical Whiteness Studies”. Being informed by a robust racial analysis of acts that reinforce white supremacy, this article will likely be perceived as not nice by those who benefit from and work to protect white supremacy. Likely, I will be the one accused of being hateful, divisive, and even racist. In order to interrogate the weaponization of this conception of “niceness”, my analysis will be driven by Critical Race Hermeneutics with white emotionality and whitelashing used as interpretive lenses. As this article’s engagement with these critical race frameworks poses a threat to those who benefit from racism, this is a dangerous, not-so-nice critical race love letter.
Model Parameters and Degradation Mechanism Analysis of Indium Phosphide Hetero-Junction Bipolar Transistors Exposed to Proton Irradiation
Authors: Zhao Xiaohong , Wang Hongwei , Zhang Yihao , Chen You , Cheng Siyi , Wang Xing , Peng Fang , Yang Yongjian , Tang Guannan , Bai Yurong , Sun Shaowei
Published: May 2024

The degradation properties of Indium phosphide hetero-junction bipolar transistors (InP HBTs) under proton irradiation are studied and modelled using a compact model for pre-irradiation, post-irradiation, and post-annealing. The variation rates of the model parameters, such as the base–emitter saturation current (ISE) and ideality factor in the ideal region (NE) in the forward Gummel characteristics, the zero-biased capacitance (Cje) and the grading factor (Mjer) in the BE junction capacitance, and the transit time parameter in the base region (Tfb), are analysed to delve into the degradation mechanism induced by proton irradiation. The displacement damage, induced by proton irradiation in the space charge region of the base–emitter junction and in the quasi-neutral bulk base region, is found to be responsible for the decrease in current gain and cut-off frequency. After annealing, the variation rates of the parameters decrease significantly compared to post-irradiation. This suggests that the recombination of unstable defects leads to a slight recovery in the degradation characteristics of InP HBTs after a period of annealing.
A Single-Output-Filter Double Dual Ćuk Converter
Authors: Robles-Campos Hector R. , Rosas-Caro Julio C. , Valderrabano-Gonzalez Antonio , Posada Johnny
Published: May 2024

This study introduces an innovative version of a recently studied converter. A Double Dual Ćuk Converter was recently studied with advantages like the possibility of designing it for achieving a low-input current ripple. The proposed converter, called the Improved Double Dual Ćuk Converter, maintains the advantages of the former one, and it is characterized by requiring one less capacitor and inductor than its predecessor. This allows addressing the challenge of optimizing the topology to reduce component count without compromising the operation; this work proposes an efficient design methodology based on theoretical analysis and experimental validation. Results demonstrate that the improved topology not only retains the advantages of the previous version, including high efficiency and robustness, but also enhances power density by reducing the number of components. These advancements open new possibilities for applications requiring compact and efficient power converters, such as renewable energy systems, electric vehicles, and portable power supply systems. This work underscores the importance of continuous innovation in power converter design and lays the groundwork for future research aimed at optimizing converter topologies. A detailed discussion of the operating principles and modeling of the converter is provided. Furthermore, simulation outcomes highlighting differences in steady-state duration, output voltage, input current ripple, and operational efficiency are shared. The results from an experimental test bench are also presented to corroborate the efficacy of the improved converter.
An Improved Suppression Method of AC Transient Overvoltage for Line Commuted Converter Based High Voltage Direct Current Considering AC-DC System Coupling
Authors: Ouyang Jinxin , Chen Yujie , Pan Xinyu , Diao Yanbo
Published: May 2024

Commutation failures in line commuted converter-based high voltage direct current (LCC-HVDC) transmission systems leads to an increase in the converter bus voltage of the rectifier station, thus resulting in AC transient overvoltage in the sending-end grid. The transient overvoltage could lead to the disconnection of renewable energy generation and threaten the stable operation of the sending-end grid. However, the influences of the coupling between AC and DC systems caused by the interaction between the active and reactive power of the sending-end grid, the AC bus voltage of the rectifier station, and the DC current are ignored. The AC transient overvoltage cannot be accurately suppressed. Therefore, in this study, the transient voltage characteristics of the rectifier station under a commutation failure of the inverter station are analyzed. The influence of LCC-HVDC control on the AC bus voltage of a rectifier station through the active and reactive power of the rectifier station is analyzed. A dynamic model of the AC bus voltage of a rectifier station under an AC-DC system coupling is established. The calculation method of the command value of the DC current of the rectifier station is proposed by a predictive control model, and an improved suppression method for AC transient overvoltage is proposed. The case studies show that the accuracy and effectiveness of the suppression of AC transient overvoltage are improved by considering the coupling between AC and DC systems.
Detecting Smell/Gas-Source Direction Using Output Voltage Characteristics of a CMOS Smell Sensor
Authors: Asada Yoshihiro , Maeno Kenichi , Hashizume Kenichi , Yodo Yusuke , Noda Toshihiko , Sawada Kazuaki , Akiyama Masahiro
Published: May 2024

Various organisms, such as dogs and moths, can locate their prey and mates by sensing their smells. Following this manner, if an engineering device with the capability to detect a smell or gas source is realized, it can have a wide range of potential applications, such as searching for landmines, locating gas leaks, and rapid detection of fire. A previous study on the estimation of smell and gas-flow direction successfully detected the smell/gas-source direction in low-wind-velocity environments using a semiconductor gas sensor array. However, some problems are generally associated with the use of semiconductor gas sensors due to the use of heaters. This study aimed to detect the location of a smell/gas source using an integrated CMOS smell sensor array, which operates at room temperature without a heater. The experiment showed that under ideal conditions, the order of gas responses and concentration gradient of the gas enabled the estimation of the direction of the smell/gas-source location on one side of the sensor.
Value-Guided Adaptive Data Augmentation for Imbalanced Small Object Detection
Authors: Wang Haipeng , Sui Chenhong , Jiang Fuhao , Li Shuai , Liu Hao , Wang Ao
Published: May 2024

Data augmentation is considered a promising technique to resolve the imbalance of large and small objects. Unfortunately, most existing methods augment all small objects indiscriminately, regardless of their learnability and proportion. This tends to result in wasteful enlargement for many weak, low-information objects but under-augmentation for rare and learnable objects. To this end, we propose a value-guided adaptive data augmentation for scale- and proportion-imbalanced small object detection (ValCopy-Paste). Specifically, we first develop a non-learning object value criteria to determine whether one object should be expanded. Both scale-based learnability and quantity-based necessity are involved in this criteria. Then, the value distribution of objects in the dataset can be further constructed on the basis of the relevant object values. This helps to ensure that those uncommon, learnable objects that deserve enhancement are more likely to be enhanced. Additionally, we propose to enhance the data by pasting the sampled objects into relatively smooth portions of fresh background images, rather than arbitrary areas of any background images. This helps to boost data diversity while reducing the interference from complicated backgrounds. Evidently, our method does not require sophisticated training and just depends on the size and distribution of the objects in the dataset. Extensive experiments on MS COCO 2017 and PASCAL VOC 2012 demonstrate that our method achieves better performance than state-of-the-art methods.
Which Diabetes Patients Will Benefit the Most from Once-Weekly Basal Insulin Analogs? A Review with a Special Focus on Type 1 Diabetes Patients
Authors: Kosmeri Chrysoula , Baltogianni Maria , Giapros Vasileios , Siomou Ekaterini , Tsinopoulou Vasiliki-Regina , Balomenou Foteini , Serbis Anastasios
Published: May 2024

Basal insulin analogs, typically administered once or twice daily, have been one of the two pillars of the multiple daily injection (MDI) insulin therapy of patients with type 1 diabetes (T1D) for the last twenty years. Recently, once-weekly basal insulin analogs have been developed and are in late-phase clinical trials. One of these analogs is insulin icodec (icodec), appropriately developed to bind reversibly to albumin and to be gradually released into the patient’s circulation. Icodec has been tried mostly in clinical trials of adult patients with type 2 diabetes. A recent phase 3a clinical trial comprising adult patients with T1D was designed to evaluate icodec’s efficacy and safety compared with a daily basal insulin analog (degludec) after a 26-week main phase plus a safety extension of another 26 weeks. Icodec showed non-inferiority to once-daily degludec in glycated hemoglobin (HbA1c) reduction at week 26, and no significant differences in time in range (TIR) (70–180 mg/dL) and in time above range (TAR) (>180 mg/dL). On the other hand, it was associated with increased rates of clinically significant hypoglycemia (blood glucose < 54 mg/dL) and severe hypoglycemia (external assistance need for recovery), remaining either below or close to the internationally recommended targets for hypoglycemia. Another once-weekly insulin analog, basal insulin Fc (BIF), has been investigated in a phase 2 clinical trial comprising adult patients with T1D, with equally promising results. These preliminary data suggest that once-weekly insulin analogs could be of use for some patients with T1D, for example, patients not taking insulin regularly or those who are on MDI and wish for fewer injections. In addition, due to its prolonged mode of action, it could decrease the risk of diabetic ketoacidosis and the need for hospitalization. Additionally, patients with T1D that struggle with wearing diabetes mellitus devices/closed-loop insulin pumps either due to the cost or due to skin issues may also benefit from long-acting insulin. There is increasing evidence of the benefits of adjunctive therapies to insulin in T1D patients, but these therapies are not FDA-approved due to a possible higher risk of diabetic ketoacidosis. These long-acting insulin analogues could be used with adjunctive therapies in selected patients. This review aims to present available data on the mode of action, clinical trial results, and possible benefits of once-weekly insulin analogs for patients with T1D. In addition, it intends to suggest a future research framework for important clinical questions, such as once-weekly insulin analog use and exercise, sick days, or surgery, that will enhance our knowledge regarding this indisputable innovation in insulin management.
PlantMine: A Machine-Learning Framework to Detect Core SNPs in Rice Genomics
Authors: Tong Kai , Chen Xiaojing , Yan Shen , Dai Liangli , Liao Yuxue , Li Zhaoling , Wang Ting
Published: May 2024

As a fundamental global staple crop, rice plays a pivotal role in human nutrition and agricultural production systems. However, its complex genetic architecture and extensive trait variability pose challenges for breeders and researchers in optimizing yield and quality. Particularly to expedite breeding methods like genomic selection, isolating core SNPs related to target traits from genome-wide data reduces irrelevant mutation noise, enhancing computational precision and efficiency. Thus, exploring efficient computational approaches to mine core SNPs is of great importance. This study introduces PlantMine, an innovative computational framework that integrates feature selection and machine learning techniques to effectively identify core SNPs critical for the improvement of rice traits. Utilizing the dataset from the 3000 Rice Genomes Project, we applied different algorithms for analysis. The findings underscore the effectiveness of combining feature selection with machine learning in accurately identifying core SNPs, offering a promising avenue to expedite rice breeding efforts and improve crop productivity and resilience to stress.
Pentoxifylline and Norcantharidin Modify p62 Expression in 2D and 3D Cultures of B16F1 Cells
Authors: González-Quiroz José Luis , Ocampo-Godínez Juan Moisés , Hernández-González Victoria Noemi , Lezama Ruth Angélica , Reyes-Maldonado Elba , Vega-López Armando , Domínguez-López María Lilia
Published: May 2024

Three-dimensional cell cultures have improved the evaluation of drugs for cancer therapy, due to their high similarity to solid tumors. In melanoma, autophagy appears to show a dual role depending on the progression of the disease. p62 protein has been proposed for the evaluation of autophagic flux since its expression is an indicator of the state of autophagy. Pentoxifylline (PTX) and Norcantharidin (NCTD) are drugs that have been shown to possess anticancer effects. In this work, we used B16F1 mouse melanoma cells in two-dimensional (2D) monolayer cultures and three-dimensional (3D) spheroids to test the effect of PTX and NCTD over the p62 expression. We analyzed the effect on p62 expression through Western blot and immunofluorescence assays. Our results indicate that PTX decreases p62 expression in both cell culture models, while Norcantharidin increases its expression in 3D cultures at 24 h. Therefore, these drugs could have a potential therapeutic use for the regulation of autophagy in melanoma, depending on the state of evolution of the disease.
Evaluation of PAC and FASP Performance: DIA-Based Quantitative Proteomic Analysis
Authors: Murfuni Maria Stella , Prestagiacomo Licia E. , Giuliano Annarita , Gabriele Caterina , Signoretti Sara , Cuda Giovanni , Gaspari Marco
Published: May 2024

The aim of this study was to compare filter-aided sample preparation (FASP) and protein aggregation capture (PAC) starting from a three-species protein mix (Human, Soybean and Pisum sativum) and two different starting amounts (1 and 10 µg). Peptide mixtures were analyzed by data-independent acquisition (DIA) and raw files were processed by three commonly used software: Spectronaut, MaxDIA and DIA-NN. Overall, the highest number of proteins (mean value of 5491) were identified by PAC (10 µg), while the lowest number (4855) was identified by FASP (1 µg). The latter experiment displayed the worst performance in terms of both specificity (0.73) and precision (0.24). Other tested conditions showed better diagnostic accuracy, with specificity values of 0.95–0.99 and precision values between 0.61 and 0.86. In order to provide guidance on the data analysis pipeline, the accuracy diagnostic of three software was investigated: (i) the highest sensitivity was obtained with Spectronaut (median of 0.67) highlighting the ability of Spectronaut to quantify low-abundance proteins, (ii) the best precision value was obtained by MaxDIA (median of 0.84), but with a reduced number of identifications compared to Spectronaut and DIA-NN data, and (iii) the specificity values were similar (between 0.93 and 0.99). The data are available on ProteomeXchange with the identifier PXD044349.
Nanoscale Interaction of Endonuclease APE1 with DNA
Authors: Vemulapalli Sridhar , Hashemi Mohtadin , Chen Yingling , Pramanik Suravi , Bhakat Kishor K. , Lyubchenko Yuri L.
Published: May 2024

Apurinic/apyrimidinic endonuclease 1 (APE1) is involved in DNA repair and transcriptional regulation mechanisms. This multifunctional activity of APE1 should be supported by specific structural properties of APE1 that have not yet been elucidated. Herein, we applied atomic force microscopy (AFM) to characterize the interactions of APE1 with DNA containing two well-separated G-rich segments. Complexes of APE1 with DNA containing G-rich segments were visualized, and analysis of the complexes revealed the affinity of APE1 to G-rich DNA sequences, and their yield was as high as 53%. Furthermore, APE1 is capable of binding two DNA segments leading to the formation of loops in the DNA–APE1 complexes. The analysis of looped APE1-DNA complexes revealed that APE1 can bridge G-rich segments of DNA. The yield of loops bridging two G-rich DNA segments was 41%. Analysis of protein size in various complexes was performed, and these data showed that loops are formed by APE1 monomer, suggesting that APE1 has two DNA binding sites. The data led us to a model for the interaction of APE1 with DNA and the search for the specific sites. The implication of these new APE1 properties in organizing DNA, by bringing two distant sites together, for facilitating the scanning for damage and coordinating repair and transcription is discussed.
Quercetin Induces Mitochondrial Apoptosis and Downregulates Ganglioside GD3 Expression in Melanoma Cells
Authors: Seo Sang Young , Ju Won Seok , Kim Kyongtae , Kim Juhwan , Yu Jin Ok , Ryu Jae-Sung , Kim Ji-Su , Lee Hyun-A , Koo Deog-Bon , Choo Young-Kug
Published: May 2024

Malignant melanoma represents a form of skin cancer characterized by a bleak prognosis and heightened resistance to traditional therapies. Quercetin has demonstrated notable anti-carcinogenic, anti-inflammatory, anti-oxidant, and pharmacological effects across various cancer types. However, the intricate relationship between quercetin’s anti-cancer properties and ganglioside expression in melanoma remains incompletely understood. In this study, quercetin manifests specific anti-proliferative, anti-migratory, and cell-cycle arrest effects, inducing mitochondrial dysfunction and apoptosis in two melanoma cancer cell lines. This positions quercetin as a promising candidate for treating malignant melanoma. Moreover, our investigation indicates that quercetin significantly reduces the expression levels of ganglioside GD3 and its synthetic enzyme. Notably, this reduction is achieved through the inhibition of the FAK/paxillin/Akt signaling pathway, which plays a crucial role in cancer development. Taken together, our findings suggest that quercetin may be a potent anti-cancer drug candidate for the treatment of malignant melanoma.
Porous Hydrogels for Immunomodulatory Applications
Authors: Wu Cuifang , Zhang Honghong , Guo Yangyang , Sun Xiaomin , Hu Zuquan , Teng Lijing , Zeng Zhu
Published: May 2024

Cancer immunotherapy relies on the insight that the immune system can be used to defend against malignant cells. The aim of cancer immunotherapy is to utilize, modulate, activate, and train the immune system to amplify antitumor T-cell immunity. In parallel, the immune system response to damaged tissue is also crucial in determining the success or failure of an implant. Due to their extracellular matrix mimetics and tunable chemical or physical performance, hydrogels are promising platforms for building immunomodulatory microenvironments for realizing cancer therapy and tissue regeneration. However, submicron or nanosized pore structures within hydrogels are not favorable for modulating immune cell function, such as cell invasion, migration, and immunophenotype. In contrast, hydrogels with a porous structure not only allow for nutrient transportation and metabolite discharge but also offer more space for realizing cell function. In this review, the design strategies and influencing factors of porous hydrogels for cancer therapy and tissue regeneration are first discussed. Second, the immunomodulatory effects and therapeutic outcomes of different porous hydrogels for cancer immunotherapy and tissue regeneration are highlighted. Beyond that, this review highlights the effects of pore size on immune function and potential signal transduction. Finally, the remaining challenges and perspectives of immunomodulatory porous hydrogels are discussed.
Cardiac Left Ventricular miRNA-26a Is Downregulated in Ovariectomized Mice, Upregulated upon 17-Beta Estradiol Replacement, and Inversely Correlated with Collagen Type 1 Gene Expression
Authors: Assayag Elishai , Gurt Irina , Cohen-Kfir Einav , Stokar Joshua , Zwas Donna R. , Dresner-Pollak Rivka
Published: May 2024

Heart failure with preserved ejection fraction (HFpEF) is more prevalent in post- compared to pre-menopausal women. The underlying mechanisms are not fully understood. Data in humans is confounded by age and co-morbidities. We investigated the effects of ovariectomy and estrogen replacement on the left ventricular (LV) gene expression of pro-inflammatory and pro-fibrotic factors involved in HFpEF and putative regulating miRNAs. Nine-week-old C57BL/6 female mice were subjected to ovariectomy (OVX) or SHAM operation. OVX and SHAM groups were sacrificed 1-, 6-, and 12-weeks post-surgery (T1/SHAM; T1/OVX; T6/SHAM; T6/OVX, T12/SHAM). 17β-estradiol (E2) or vehicle (VEH) was then administered to the OVX groups for 6 weeks (T12/OVX/E2; T12/OVX/VEH). Another SHAM group was sacrificed 12-weeks post-surgery. RNA and miRNAs were extracted from the LV apex. An early 3-fold increase in the gene expression of IL-1α, IL-6, Mmp9, Mmp12, Col1α1, and Col3α1 was observed one-week post-surgery in T1/OVX vs. T1/SHAM, but not at later time points. miRNA-26a was lower in T1/OVX vs. T1/SHAM and was inversely correlated with Col1α1 and Col3α1 expression 1-week post-surgery (r = −0.79 p < 0.001; r = −0.6 p = 0.007). miRNAs-26a, 29b, and 133a were significantly higher, while Col1α1, Col3α1, IL-1α, IL-6, Tnfα, Mmp12, and FasL gene expression was significantly lower in E2- compared to vehicle-treated OVX mice. miRNA-26a was inversely correlated with Col3α1 in T12/OVX/ E2 (r = −0.56 p = 0.02). OVX triggered an early increase in the gene expression of pro-inflammatory and pro-fibrotic factors, highlighting the importance of the early phase post-cessation of ovarian function. E2 replacement therapy, even if it was not immediately initiated after OVX, reversed these unfavorable changes and upregulated cardiac miRNA-26a, previously unknown to be affected by menopausal status.
Diagnosis of Fabry Disease Using Alpha-Galactosidase A Activity or LysoGb3 in Blood Fails to Identify Up to Two Thirds of Female Patients
Authors: Duro Giovanni , Anania Monia , Zizzo Carmela , Francofonte Daniele , Giacalone Irene , D’Errico Annalisa , Marsana Emanuela Maria , Colomba Paolo
Published: May 2024

Anderson–Fabry disease is a lysosomal storage disorder caused by mutations in the GLA gene, which encodes the enzyme α-galactosidase A. The GLA gene is located on the X-chromosome, causing an X-linked pathology: due to lyonization, female patients usually manifest a variable symptomatology, ranging from asymptomatic to severe phenotypes. The confirmation of the clinical diagnosis of Fabry disease, achieved by measuring α-galactosidase A activity, which is usually the first test used, shows differences between male and female patients. This assay is reliable in male patients with causative mutations in the GLA gene, in whom the enzymatic activity is lower than normal values; on the other hand, in female Fabry patients, the enzymatic activity is extremely variable between normal and pathological values. These fluctuations are also found in female patients’ blood levels of globotriaosylsphingosine (LysoGb3) for the same reason. In this paper, we present a retrospective study conducted in our laboratories on 827 Fabry patients with causative mutations in the GLA gene. Our results show that 100% of male patients had α-galactosidase A activity below the reference value, while more than 70% of female patients had normal values. It can also be observed that almost half of the female patients with pathogenic mutations in the GLA gene showed normal values of LysoGb3 in blood. Furthermore, in women, blood LysoGb3 values can vary over time, as we show in a clinical case presented in this paper. Both these tests could lead to missed diagnoses of Fabry disease in female patients, so the analysis of the GLA gene represents the main diagnostic test for Fabry disease in women to date.