bluuebunny/crossref_metadata_2025 huggingface dataset:
Feature-Level Fusion between Gaofen-5 and Sentinel-1A Data for Tea Plantation Mapping
Authors: Chen Yujia , Tian Shufang
Published: December 2020

The accurate mapping of tea plantations is significant for government decision-making and environmental protection of tea-producing regions. Hyperspectral and Synthetic Aperture Radar (SAR) data have recently been widely used in land cover classification, but effective integration of these data for tea plantation mapping requires further study. This study developed a new feature-level image fusion method called LPPSubFus that combines locality preserving projection and subspace fusion (SubFus) to map tea plantations. Based on hyperspectral and SAR data, we first extracted spectral indexes, textures, and backscattering information. Second, this study applied LPPSubFus to tea plantation mapping with different classification algorithms. Finally, we compared the performance of LPPSubFus, SubFus, and pixel-level image fusion in tea plantation mapping. Feature-level image fusion performed better than pixel-level image fusion. An improvement of about 3% was achieved using feature-level image fusion compared to hyperspectral data alone. Regarding feature-level image fusion, LPPSubFus improved the overall accuracy by more than 3% compared to SubFus. In particular, LPPSubFus using neural network algorithms achieved the highest overall accuracy (95%) and over 90% producer and user accuracy for tea plantations and forests. In addition, LPPSubFus was more compatible with different classification algorithms than SubFus. Based on these findings, it is concluded that LPPSubFus has better and more stable performance in tea plantation mapping than pixel-level image fusion and SubFus. This study demonstrates the potential of integrating hyperspectral and SAR data via LPPSubFus for mapping tea plantations. Our work offers a promising tea plantation mapping method and contributes to the understanding of hyperspectral and SAR data fusion.
Stomatal and Leaf Morphology Response of European Beech (Fagus sylvatica L.) Provenances Transferred to Contrasting Climatic Conditions
Authors: Petrík Peter , Petek Anja , Konôpková Alena , Bosela Michal , Fleischer Peter , Frýdl Josef , Kurjak Daniel
Published: December 2020

Climate change-induced elevated temperatures and drought are considered to be serious threats to forest ecosystems worldwide, negatively affecting tree growth and viability. We studied nine European beech (Fagus sylvatica L.) provenances located in two provenance trial plots with contrasting climates in Central Europe. Stomata play a vital role in the water balance of plants by regulating gaseous exchanges between plants and the atmosphere. Therefore, to explain the possible adaptation and acclimation of provenances to climate conditions, stomatal (stomatal density, the length of guard cells, and the potential conductance index) and leaf morphological traits (leaf size, leaf dry weight and specific leaf area) were assessed. The phenotypic plasticity index was calculated from the variability of provenances’ stomatal and leaf traits between the provenance plots. We assessed the impact of various climatic characteristics and derived indices (e.g., ecodistance) on intraspecific differences in stomatal and leaf traits. Provenances transferred to drier and warmer conditions acclimated through a decrease in stomatal density, the length of guard cells, potential conductance index, leaf size and leaf dry weight. The reduction in stomatal density and the potential conductance index was proportional to the degree of aridity difference between the climate of origin and conditions of the new site. Moreover, we found that the climate heterogeneity and latitude of the original provenance sites influence the phenotypic plasticity of provenances. Provenances from lower latitudes and less heterogeneous climates showed higher values of phenotypic plasticity. Furthermore, we observed a positive correlation between phenotypic plasticity and mortality in the arid plot but not in the more humid plot. Based on these impacts of the climate on stomatal and leaf traits of transferred provenances, we can improve the predictions of provenance reactions for future scenarios of global climate change.
Ground Response and Historical Buildings in Avellino (Campania, Southern Italy): Clues from a Retrospective View Concerning the 1980 Irpinia-Basilicata Earthquake
Authors: Nardone Lucia , Gizzi Fabrizio Terenzio , Maresca Rosalba
Published: December 2020

Cultural heritage represents our legacy with the past and our identity. However, to assure heritage can be passed on to future generations, it is required to put into the field knowledge as well as preventive and safeguard actions, especially for heritage located in seismic hazard-prone areas. With this in mind, the article deals with the analysis of ground response in the Avellino town (Campania, Southern Italy) and its correlation with the effects caused by the 23rd November 1980 Irpinia earthquake on the historical buildings. The aim is to get some clues about the earthquake damage cause-effect relationship. To estimate the ground motion response for Avellino, where strong-motion recordings are not available, we made use of the seismic hazard disaggregation. Then, we made extensive use of borehole data to build the lithological model so being able to assess the seismic ground response. Overall, results indicate that the complex subsoil layers influence the ground motion, particularly in the lowest period (0.1–0.5 s). The comparison with the observed damage of the selected historical buildings and the maximum acceleration expected indicates that the damage distribution cannot be explained by the surface geology effects alone.
Bilateral Nephroblastoma with Dilated Cardiomyopathy as an Indication for Off-Protocol Treatment: A Case Report
Authors: Sosnowska-Sienkiewicz Patrycja , Gowin Ewelina , Jończyk-Potoczna Katarzyna , Mańkowski Przemysław , Godziński Jan , Januszkiewicz-Lewandowska Danuta
Published: December 2020

Patients with a Wilms tumor are often admitted to the hospital accidentally, with an abdominal mass causing asymmetry of the abdominal wall. Hypertension accompanying a Wilms tumor occurs in about 10–27% of children, but cardiomyopathy associated with a Wilms tumor is very rarely described. This publication presents a case of a 9-month-old girl with a bilateral Wilms tumor accompanied by dilated cardiomyopathy since her initial cancer diagnosis, as well as her off-protocol treatment. The severe condition of the child forced the application of off-protocol treatment, i.e., accelerated resection of a larger tumor, which enabled the improvement of heart performance and made subsequent therapy possible. In the course of the presented treatment, a gradual normalization of cardiac ventricular function and contractility was observed. In conclusion, a massive abdominal tumor associated with abdominal compartment syndrome compromised the functioning of the cardiovascular system in the young child. Therefore, earlier removal of Wilms tumors in patients with heart failure should be considered. This may result in the improvement of cardiovascular function and the possibility of further therapy.
Predicting Length of Stay and Discharge Destination for Surgical Patients: A Cohort Study
Authors: Bert Fabrizio , Kakaa Omar , Corradi Alessio , Mascaro Annamaria , Roggero Stefano , Corsi Daniela , Scarmozzino Antonio , Siliquini Roberta
Published: December 2020

Discharge planning is important to prevent surgical site infections, reduce costs, and improve the hospitalization experience. The identification of early variables that can predict a longer-than-expected length of stay or the need for a discharge with additional needs can improve this process. A cohort study was conducted in the largest hospital of Northern Italy, collecting discharge records from January 2017 to January 2020 and pre-admission visits in the last three months. Socio-demographic and clinical data were collected. Linear and logistic regression models were fitted. The main outcomes were the length of stay (LOS) and discharge destination. The main predictors of a longer LOS were the need for additional care at discharge (+10.76 days), hospitalization from the emergency department (ED) (+5.21 days), and age (+0.04 days per year), accounting for clinical variables (p < 0.001 for all variables). Each year of age and hospitalization from the ED were associated with a higher probability of needing additional care at discharge (OR 1.02 and 1.77, respectively, p < 0.001). No additional findings came from pre-admission forms. Discharge difficulties seem to be related mainly to age and hospitalization procedures: those factors are probably masking underlying social risk factors that do not show up in patients with planned admissions.
Erratum: Jung, S., et al. Cluster-Based Analysis of Infectious Disease Occurrences Using Tensor Decomposition: A Case Study of South Korea. Int. J. Environ. Res. Public Health 2020, 17, 4872
Authors: Jung Seungwon , Moon Jaeuk , Hwang Eenjun
Published: December 2020

The source of funding should be modified in our original article [...]
Evolution of Urban Haze in Greater Bangkok and Association with Local Meteorological and Synoptic Characteristics during Two Recent Haze Episodes
Authors: Aman Nishit , Manomaiphiboon Kasemsan , Pala-En Natchanok , Kokkaew Eakkachai , Boonyoo Tassana , Pattaramunikul Suchart , Devkota Bikash , Chotamonsak Chakrit
Published: December 2020

This present work investigates several local and synoptic meteorological aspects associated with two wintertime haze episodes in Greater Bangkok using observational data, covering synoptic patterns evolution, day-to-day and diurnal variation, dynamic stability, temperature inversion, and back-trajectories. The episodes include an elevated haze event of 16 days (14–29 January 2015) for the first episode and 8 days (19–26 December 2017) for the second episode, together with some days before and after the haze event. Daily PM2.5 was found to be 50 µg m−3 or higher over most of the days during both haze events. These haze events commonly have cold surges as the background synoptic feature to initiate or trigger haze evolution. A cold surge reached the study area before the start of each haze event, causing temperature and relative humidity to drop abruptly initially but then gradually increased as the cold surge weakened or dissipated. Wind speed was relatively high when the cold surge was active. Global radiation was generally modulated by cloud cover, which turns relatively high during each haze event because cold surge induces less cloud. Daytime dynamic stability was generally unstable along the course of each haze event, except being stable at the ending of the second haze event due to a tropical depression. In each haze event, low-level temperature inversion existed, with multiple layers seen in the beginning, effectively suppressing atmospheric dilution. Large-scale subsidence inversion aloft was also persistently present. In both episodes, PM2.5 showed stronger diurnality during the time of elevated haze, as compared to the pre- and post-haze periods. During the first episode, an apparent contrast of PM2.5 diurnality was seen between the first and second parts of the haze event with relatively low afternoon PM2.5 over its first part, but relatively high afternoon PM2.5 over its second part, possibly due to the role of secondary aerosols. PM2.5/PM10 ratio was relatively lower in the first episode because of more impact of biomass burning, which was in general agreement with back-trajectories and active fire hotspots. The second haze event, with little biomass burning in the region, was likely to be caused mainly by local anthropogenic emissions. These findings suggest a need for haze-related policymaking with an integrated approach that accounts for all important emission sectors for both particulate and gaseous precursors of secondary aerosols. Given that cold surges induce an abrupt change in local meteorology, the time window to apply control measures for haze is limited, emphasizing the need for readiness in mitigation responses and early public warning.
An Association between Rainy Days with Clinical Dengue Fever in Dhaka, Bangladesh: Findings from a Hospital Based Study
Authors: Rahman Kazi Mizanur , Sharker Yushuf , Rumi Reza Ali , Khan Mahboob-Ul Islam , Shomik Mohammad Sohel , Rahman Muhammad Waliur , Billah Sk Masum , Rahman Mahmudur , Streatfield Peter Kim , Harley David , Luby Stephen P.
Published: December 2020

Background: Dengue, a febrile illness, is caused by a Flavivirus transmitted by Aedes aegypti and Aedes albopictus mosquitoes. Climate influences the ecology of the vectors. We aimed to identify the influence of climatic variability on the occurrence of clinical dengue requiring hospitalization in Zone-5, a high incidence area of Dhaka City Corporation (DCC), Bangladesh. Methods and Findings: We retrospectively identified clinical dengue cases hospitalized from Zone-5 of DCC between 2005 and 2009. We extracted records of the four major catchment hospitals of the study area. The Bangladesh Meteorological Department (BMD) provided data on temperature, rainfall, and humidity of DCC for the study period. We used autoregressive integrated moving average (ARIMA) models for the number of monthly dengue hospitalizations. We also modeled all the climatic variables using Poisson regression. During our study period, dengue occurred throughout the year in Zone-5 of DCC. The median number of hospitalized dengue cases was 9 per month. Dengue incidence increased sharply from June, and reached its peak in August. One additional rainy day per month increased dengue cases in the succeeding month by 6% (RR = 1.06, 95% CI: 1.04–1.09). Conclusions: Dengue is transmitted throughout the year in Zone-5 of DCC, with seasonal variation in incidence. The number of rainy days per month is significantly associated with dengue incidence in the subsequent month. Our study suggests the initiation of campaigns in DCC for controlling dengue and other Aedes mosquito borne diseases, including Chikunguniya from the month of May each year. BMD rainfall data may be used to determine campaign timing.
Medication-Related Osteonecrosis of the Jaws and CDK4/6 Inhibitors: A Recent Association
Authors: Marcianò Antonia , Guzzo Gian Marco , Peditto Matteo , Picone Antonio , Oteri Giacomo
Published: December 2020

The purpose of the present study was to estimate the prevalence of cyclin-dependent kinase (CDK) 4/6 inhibitors use among cancer patients from the medication-related osteonecrosis of the jaw (MRONJ) cohort of the University of Messina. We retrospectively reviewed the records of all patients with either intravenous bisphosphonates or denosumab-related MRONJ reported in the electronic health records of the Unit of Oral Surgery, School of Dentistry, University of Messina between the first quarter of 2018 and the first quarter 2020 to identify eligible patients. We observed six cases of MRONJ associated with CDK4/6 inhibitors concomitantly with intravenous bisphosphonates and/or denosumab in breast cancer patients. The CDK4/6 inhibitors registered were palbociclib (n = 5) and abemaciclib (n = 1). Data of cancer patients diagnosed with MRONJ in the same period (n = 10) were extracted for comparison. The comparative assessment with this group of patients showed a similar distribution of MRONJ stage ranged and clinical course after treatment. The degree of risk for osteonecrosis in patients taking these new classes of drugs is uncertain but warrants awareness and close monitoring. The role of premedication dental evaluation as a prevention strategy has been acknowledged for cancer patients about to initiate intravenous bisphosphonates and/or denosumab for treatment of bone metastasis, but additional attention should be paid to whom are assuming CDK4/6 inhibitors because of their oral adverse events.
Community-Level Factors Associated with COVID-19 Cases and Testing Equity in King County, Washington
Authors: Seto Edmund , Min Esther , Ingram Carolyn , Cummings BJ , Farquhar Stephanie A.
Published: December 2020

Individual-level Coronavirus Disease 2019 (COVID-19) case data suggest that certain populations may be more impacted by the pandemic. However, few studies have considered the communities from which positive cases are prevalent, and the variations in testing rates between communities. In this study, we assessed community factors that were associated with COVID-19 testing and test positivity at the census tract level for the Seattle, King County, Washington region at the summer peak of infection in July 2020. Multivariate Poisson regression was used to estimate confirmed case counts, adjusted for testing numbers, which were associated with socioeconomic status (SES) indicators such as poverty, educational attainment, transportation cost, as well as with communities with high proportions of people of color. Multivariate models were also used to examine factors associated with testing rates, and found disparities in testing for communities of color and communities with transportation cost barriers. These results demonstrate the ability to identify tract-level indicators of COVID-19 risk and specific communities that are most vulnerable to COVID-19 infection, as well as highlight the ongoing need to ensure access to disease control resources, including information and education, testing, and future vaccination programs in low-SES and highly diverse communities.
Evolution of Protein Structure and Stability in Global Warming
Authors: Barik Sailen
Published: December 2020

This review focuses on the molecular signatures of protein structures in relation to evolution and survival in global warming. It is based on the premise that the power of evolutionary selection may lead to thermotolerant organisms that will repopulate the planet and continue life in general, but perhaps with different kinds of flora and fauna. Our focus is on molecular mechanisms, whereby known examples of thermoresistance and their physicochemical characteristics were noted. A comparison of interactions of diverse residues in proteins from thermophilic and mesophilic organisms, as well as reverse genetic studies, revealed a set of imprecise molecular signatures that pointed to major roles of hydrophobicity, solvent accessibility, disulfide bonds, hydrogen bonds, ionic and π-electron interactions, and an overall condensed packing of the higher-order structure, especially in the hydrophobic regions. Regardless of mutations, specialized protein chaperones may play a cardinal role. In evolutionary terms, thermoresistance to global warming will likely occur in stepwise mutational changes, conforming to the molecular signatures, such that each “intermediate” fits a temporary niche through punctuated equilibrium, while maintaining protein functionality. Finally, the population response of different species to global warming may vary substantially, and, as such, some may evolve while others will undergo catastrophic mass extinction.
Improving the Performance of Multiobjective Genetic Algorithms: An Elitism-Based Approach
Authors: Guariso Giorgio , Sangiorgio Matteo
Published: December 2020

Today, many complex multiobjective problems are dealt with using genetic algorithms (GAs). They apply the evolution mechanism of a natural population to a “numerical” population of solutions to optimize a fitness function. GA implementations must find a compromise between the breath of the search (to avoid being trapped into local minima) and its depth (to prevent a rough approximation of the optimal solution). Most algorithms use “elitism”, which allows preserving some of the current best solutions in the successive generations. If the initial population is randomly selected, as in many GA packages, the elite may concentrate in a limited part of the Pareto frontier preventing its complete spanning. A full view of the frontier is possible if one, first, solves the single-objective problems that correspond to the extremes of the Pareto boundary, and then uses such solutions as elite members of the initial population. The paper compares this approach with more conventional initializations by using some classical tests with a variable number of objectives and known analytical solutions. Then we show the results of the proposed algorithm in the optimization of a real-world system, contrasting its performances with those of standard packages.
The Healthy Pregnancy Service to Optimise Excess Gestational Weight Gain for Women with Obesity: A Qualitative Study of Health Professionals’ Perspectives
Authors: Goldstein Rebecca F. , Walker Ruth E. , Teede Helena J. , Harrison Cheryce L. , Boyle Jacqueline A.
Published: December 2020

Maternal obesity is associated with health risks for women and their babies, exacerbated by excess gestational weight gain. We describe health professionals’ perspectives in the provision of a Healthy Pregnancy service designed to optimise healthy lifestyle and support recommended gestational weight gain for women with obesity. Semi-structured interviews were conducted with health professionals. Questions were based on the Theoretical Domains Framework (TDF) and deductive thematic analysis was performed. A total of 14 multidisciplinary staff were interviewed. Six themes were identified: 1. health professionals view themselves as part of a team; 2. health professionals reported having necessary skills; 3. experience generated confidence in discussing gestational weight gain; 4. gestational weight gain is considered of variable importance; 5. health professionals want women to be comfortable; 6. the environmental context and resources presented some barriers. Staff were supportive of the Healthy Pregnancy service and valued developing teamwork with staff and rapport with women. Most felt relatively comfortable discussing weight gain with women. Barriers included ability to navigate sensitive topics with women, limited awareness of the intervention among new staff, communication between teams, and waiting time for women. Barriers and enablers to the delivery of an integrated model of maternity care were identified. These findings should inform and improve implementation of service models integrating healthy lifestyle in the antenatal care of women with obesity.
Imaging of Joints and Bones in Autoinflammation
Authors: Ziegeler Katharina , Eshed Iris , Diekhoff Torsten , Hermann Kay Geert
Published: December 2020

Autoinflammatory disorders are commonly characterized by seemingly unprovoked systemic inflammation mainly driven by cells and cytokines of the innate immune system. In many disorders on this spectrum, joint and bone involvement may be observed and imaging of these manifestations can provide essential diagnostic information. This review aimed to provide a comprehensive overview of the imaging characteristics for major diseases and disease groups on the autoinflammatory spectrum, including familial Mediterranean fever (FMF), Behçet disease (BD), crystal deposition diseases (including gout), adult-onset Still’s disease (AoSD), and syndromatic synovitis, acne, pustulosis, hyperostosis, and osteitis (SAPHO)/chronic recurrent multifocal osteomyelitis (CRMO). Herein, we discuss common and distinguishing imaging characteristics, phenotypical overlaps with related diseases, and promising fields of future research.
Addition of Lymphatic Stimulating Self-Care Practices Reduces Acute Attacks among People Affected by Moderate and Severe Lower-Limb Lymphedema in Ethiopia, a Cluster Randomized Controlled Trial
Authors: Douglass Jan , Hailekiros Fikre , Martindale Sarah , Mableson Hayley , Seife Fikre , Bishaw Tesfahun , Nigussie Mekdes , Meribo Kadu , Tamiru Mossie , Agidew Getnet , Kim Susan , Betts Hannah , Taylor Mark , Kelly-Hope Louise
Published: December 2020

Lymphedema causes disability and exacerbates poverty in many countries. The management of lymphatic filariasis (LF) and podoconiosis-related lymphedema involves daily hygiene to reduce secondary infections, but self-massage and deep-breathing, which have proven beneficial in cancer-related lymphedema, are not included. A cluster randomized trial in northern Ethiopia investigated the effects of lymphatic stimulation for people affected by moderate to severe lymphedema. Participants were allocated to either standard (control n = 59) or enhanced (intervention n = 67) self-care groups. Primary outcomes were lymphedema stage, mid-calf circumference, and tissue compressibility. Secondary outcomes were the frequency and duration of acute attacks. After 24 weeks, fewer patients were assessed as severe (control −37.8%, intervention −42.4%, p = 0.15) and there were clinically relevant changes in mid-calf tissue compressibility but not circumference. There was a significant between-group difference in patients who reported any acute attacks over the study period (control n = 22 (38%), intervention n = 7 (12%), p = 0.014). Daily lymphedema self-care resulted in meaningful benefits for all participants with a greater reduction in acute episodes among people performing lymphatic stimulation. Observations of a change in lymphedema status support earlier findings in Bangladesh and extend the demonstrated benefits of enhanced self-care to people affected by podoconiosis.
Influence of Vertical Loading on Behavior of Laterally Loaded Foundation Piles: A Review
Authors: Li Qiang , Prendergast Luke J. , Askarinejad Amin , Gavin Ken
Published: December 2020

The majority of installed offshore wind turbines are supported on large-diameter, open-ended steel pile foundations, known as monopiles. These piles are subjected to vertical and lateral loads while in service. In current design practice, interaction of vertical and lateral loads are not considered, rather piles are designed to resist vertical and lateral loads independently. Whilst interaction effects are widely studied for shallow foundations, the limited research on this topic for pile foundations often produces conflicting results. This paper reviews the research of the influence of vertical loading on the lateral response of pile foundations under combined loads, from the perspective of analytical research, numerical research, and experimental research from tests performed on 1-g (gravitational acceleration) model, centrifuge, and full-scale piles. The potential reasons for the differences among the results of previous research are discussed. Some guidance for future research on the effect of vertical loads on the lateral response of piles is provided.
Cerebrospinal Fluid Penetration and Combination Therapy of Entrectinib for Disseminated ROS1/NTRK-Fusion Positive Pediatric High-Grade Glioma
Authors: Mayr Lisa , Guntner Armin S. , Madlener Sibylle , Schmook Maria T. , Peyrl Andreas , Azizi Amedeo A. , Dieckmann Karin , Reisinger Dominik , Stepien Natalia M. , Schramm Kathrin , Laemmerer Anna , Jones David T. W. , Ecker Jonas , Sahm Felix , Milde Till , Pajtler Kristian W. , Blattner-Johnson Mirjam , Strbac Miroslav , Dorfer Christian , Czech Thomas , Kirchhofer Dominik , Gabler Lisa , Berger Walter , Haberler Christine , Müllauer Leonhard , Buchberger Wolfgang , Slavc Irene , Lötsch-Gojo Daniela , Gojo Johannes
Published: December 2020

Targeting oncogenic fusion-genes in pediatric high-grade gliomas (pHGG) with entrectinib has emerged as a highly promising therapeutic approach. Despite ongoing clinical studies, to date, no reports on the treatment of cerebrospinal fluid (CSF) disseminated fusion-positive pHGG exist. Moreover, clinically important information of combination with other treatment modalities such as intrathecal therapy, radiotherapy and other targeted agents is missing. We report on our clinical experience of entrectinib therapy in two CSF disseminated ROS1/NTRK-fusion-positive pHGG cases. Combination of entrectinib with radiotherapy or intrathecal chemotherapy appears to be safe and has the potential to act synergistically with entrectinib treatment. In addition, we demonstrate CSF penetrance of entrectinib for the first time in patient samples suggesting target engagement even upon CSF dissemination. Moreover, in vitro analyses of two novel cell models derived from one case with NTRK-fusion revealed that combination therapy with either a MEK (trametinib) or a CDK4/6 (abemaciclib) inhibitor synergistically enhances entrectinib anticancer effects. In summary, our comprehensive study, including clinical experience, CSF penetrance and in vitro data on entrectinib therapy of NTRK/ROS1-fusion-positive pHGG, provides essential clinical and preclinical insights into the multimodal treatment of these highly aggressive tumors. Our data suggest that combined inhibition of NTRK/ROS1 and other therapeutic vulnerabilities enhances the antitumor effect, which should be followed-up in further preclinical and clinical studies.
Agroforestry as Policy Option for Forest-Zone Oil Palm Production in Indonesia
Authors: Purwanto Edi , Santoso Hery , Jelsma Idsert , Widayati Atiek , Nugroho Hunggul Y. S. H. , van Noordwijk Meine
Published: December 2020

With 15–20% of Indonesian oil palms located, without a legal basis and permits, within the forest zone (‘Kawasan hutan’), international concerns regarding deforestation affect the totality of Indonesian palm oil export. ‘Forest zone oil palm’ (FZ-OP) is a substantive issue that requires analysis and policy change. While spatial details of FZ-OP remain contested, we review literature on (1) the legal basis of the forest zone and its conversion, (2) social stratification in oil palm production (large-scale, plasma and independent growers), and (3) environmental consequences of forest conversion to FZ-OP, before discussing policy options in a range of social and ecological contexts. Policy options range from full regularization (as FZ-OP stands could meet international forest definitions), to conditional acceptance of diversified smallholder plantings in ‘agroforestry concessions’, to gradually phasing out FZ-OP and eviction/destruction. A nuanced and differentiated approach to FZ-OP is needed, as certification of legality along supply chains is vulnerable to illegal levies and corruption. Corporate actors trading internationally can avoid use of uncertified raw materials, effectively shifting blame and depressing farmgate prices for domestic-market palm oil, but this will not return forest conditions or stop further forest conversion. We discuss an agenda for follow-up policy research.
Ultrasound-Assisted Through-Mask Electrochemical Machining of Hole Arrays in ODS Superalloy
Authors: Wang Guoqian , Zhang Yan , Li Hansong , Tang Jian
Published: December 2020

Micro-hole arrays have found wide applications in aerospace, precision instruments, and biomedicine. Among various methods of their production, including mechanical, laser, and electrical discharge, electrochemical machining (ECM) is considered the most lucrative due to its wide processing range, high surface quality, and excellent productivity. In particular, ultrasound-assisted through-mask ECM exhibits an enhanced machining precision due to ultrasonic cavitation, which promotes the removal of the electrolytic products and bubbles. In this study, the equation of cavitation bubble oscillation was derived and numerically solved to study the influence of six different parameters on the ultrasonic cavitation and electrolysis process, and their optimal values were determined. The feasibility of the proposed ultrasound-assisted through-mask ECM technology with the optimized parameters was experimentally corroborated by the fabrication of a high-quality hole array in an oxide dispersion strengthened (ODS) MA956 superalloy.
Selective Laser Melting of Patient Individualized Osteosynthesis Plates—Digital to Physical Process Chain
Authors: Edelmann André , Dubis Monique , Hellmann Ralf
Published: December 2020

We report on the exemplified realization of a digital to physical process chain for a patient individualized osteosynthesis plate for the tarsal bone area. Anonymized patient-specific data of the right feet were captured by computer tomography, which were then digitally processed to generate a surface file format (standard tessellation language, STL) ready for additive manufacturing. Physical realization by selective laser melting in titanium using optimized parameter settings and post-processing by stress relief annealing results in a customized osteosynthesis plate with superior properties fulfilling medical demands. High fitting accuracy was demonstrated by applying the osteosynthesis plate to an equally good 3D printed bone model, which likewise was generated using the patient-specific computer tomography (CT) data employing selective laser sintering and polyamid 12. Proper fixation has been achieved without any further manipulation of the plate using standard screws, proving that based on CT data, individualized implants well adapted to the anatomical conditions can be accomplished without the need for additional steps, such as bending, cutting and shape trimming of precast bone plates during the surgical intervention. Beyond parameter optimization for selective laser melting, this exemplified digital to physical process chain highlights the potential of additive manufacturing for individualized osteosynthesis.
Performance Analysis of Polymer Electrolyte Membrane Water Electrolyzer Using OpenFOAM®: Two-Phase Flow Regime, Electrochemical Model
Authors: Rho Kyu Heon , Na Youngseung , Ha Taewook , Kim Dong Kyu
Published: December 2020

In this study, an electrochemical model was incorporated into a two-phase model using OpenFOAM® (London, United Kingdom) to analyze the two-phase flow and electrochemical behaviors in a polymer electrolyte membrane water electrolyzer. The performances of serpentine and parallel designs are compared. The current density and overpotential distribution are analyzed, and the volume fractions of oxygen and hydrogen velocity are studied to verify their influence on the current density. The current density decreases sharply when oxygen accumulates in the porous transport layer. Therefore, the current density increased sharply by 3000 A/m2 at an operating current density of 10,000 A/m2. Maldistribution of the overpotential is also observed. Second, we analyze the behaviors according to the current density. At a low current density, most of the oxygen flows out of the electrolyzer. Therefore, the decrease in performance is low. However, the current density is maldistributed when it is high, which results in decreased performance. The current density increases abruptly by 12,000 A/m2. Finally, the performances of the parallel and serpentine channels are analyzed. At a high current density, the performance of the serpentine channel is higher than that of the parallel channel by 0.016 V.
A Compact Review of Laser Welding Technologies for Amorphous Alloys
Authors: Qiao Jian , Yu Peng , Wu Yanxiong , Chen Taixi , Du Yixin , Yang Jingwei
Published: December 2020

Amorphous alloys have emerged as important materials for precision machinery, energy conversion, information processing, and aerospace components. This is due to their unique structure and excellent properties, including superior strength, high elasticity, and excellent corrosion resistance, which have attracted the attention of many researchers. However, the size of the amorphous alloy components remains limited, which affects industrial applications. Significant developments in connection with this technology are urgently needed. Laser welding represents an efficient welding method that uses a laser beam with high energy-density for heating. Laser welding has gradually become a research hotspot as a joining method for amorphous alloys due to its fast heating and cooling rates. In this compact review, the current status of research into amorphous-alloy laser welding technology is discussed, the influence of technological parameters and other welding conditions on welding quality is analyzed, and an outlook on future research and development is provided. This paper can serve as a useful reference for both fundamental research and engineering applications in this field.
Cellulose Dissolution in Mixtures of Ionic Liquids and Dimethyl Sulfoxide: A Quantitative Assessment of the Relative Importance of Temperature and Composition of the Binary Solvent
Authors: Dignani Marcella T. , Bioni Thaís A. , Paixão Thiago R. L. C. , El Seoud Omar A.
Published: December 2020

We studied the dissolution of microcrystalline cellulose (MCC) in binary mixtures of dimethyl sulfoxide (DMSO) and the ionic liquids: allylbenzyldimethylammonium acetate; 1-(2-methoxyethyl)-3-methylimidazolium acetate; 1,8-diazabicyclo [5.4.0]undec-7-ene-8-ium acetate; tetramethylguanidinium acetate. Using chemometrics, we determined the dependence of the mass fraction (in %) of dissolved cellulose (MCC-m%) on the temperature, T = 40, 60, and 80 °C, and the mole fraction of DMSO, χDMSO = 0.4, 0.6, and 0.8. We derived equations that quantified the dependence of MCC-m% on T and χDMSO. Cellulose dissolution increased as a function of increasing both variables; the contribution of χDMSO was larger than that of T in some cases. Solvent empirical polarity was qualitatively employed to rationalize the cellulose dissolution efficiency of the solvent. Using the solvatochromic probe 2,6-dichloro-4-(2,4,6-triphenylpyridinium-1-yl)phenolate (WB), we calculated the empirical polarity ET(WB) of cellobiose (a model for MCC) in ionic liquid (IL)–DMSO mixtures. The ET(WB) correlated perfectly with T (fixed χDMSO) and with χDMSO (fixed T). These results show that there is ground for using medium empirical polarity to assess cellulose dissolution efficiency. We calculated values of MCC-m% under conditions other than those employed to generate the statistical model and determined the corresponding MCC-m% experimentally. The excellent agreement between both values shows the robustness of the statistical model and the usefulness of our approach to predict cellulose dissolution, thus saving time, labor, and material.
Sea Anemone Heteractis crispa Actinoporin Demonstrates In Vitro Anticancer Activities and Prevents HT-29 Colorectal Cancer Cell Migration
Authors: Kvetkina Aleksandra , Malyarenko Olesya , Pavlenko Aleksandra , Dyshlovoy Sergey , von Amsberg Gunhild , Ermakova Svetlana , Leychenko Elena
Published: December 2020

Actinoporins are the most abundant group of sea anemone cytolytic toxins. Their membranolytic activity is of high interest for the development of novel anticancer drugs. However, to date the activity of actinoporins in malignant cells has been poorly studied. Here, we report on recombinant analog of Hct-S3 (rHct-S3), belonging to the combinatory library of Heteractis crispa actinoporins. rHct-S3 exhibited cytotoxic activity against breast MDA-MB-231 (IC50 = 7.3 µM), colorectal HT-29 (IC50 = 6.8 µM), and melanoma SK-MEL-28 (IC50 = 8.3 µM) cancer cells. The actinoporin effectively prevented epidermal growth factor -induced neoplastic transformation of JB6 Cl41 cells by 34% ± 0.2 and decreased colony formation of HT-29 cells by 47% ± 0.9, MDA-MB-231 cells by 37% ± 1.2, and SK-MEL-28 cells by 34% ± 3.6. Moreover, rHct-S3 decreased proliferation and suppressed migration of colorectal carcinoma cells by 31% ± 5.0 and 99% ± 6.4, respectively. The potent anti-migratory activity was proposed to mediate by decreased matrix metalloproteinases-2 and -9 expression. In addition, rHct-S3 induced programmed cell death by cleavage of caspase-3 and poly (ADP-ribose) polymerase, as well as regulation of Bax and Bcl-2. Our results indicate rHct-S3 to be a promising anticancer drug with a high anti-migratory potential.
GPCR Activation States Induced by Nanobodies and Mini-G Proteins Compared by NMR Spectroscopy
Authors: Rößler Philip , Mayer Daniel , Tsai Ching-Ju , Veprintsev Dmitry B. , Schertler Gebhard F. X. , Gossert Alvar D.
Published: December 2020

In this work, we examine methyl nuclear magnetic resonance (NMR) spectra of the methionine ε-[13CH3] labelled thermostabilized β1 adrenergic receptor from turkey in association with a variety of different effectors, including mini-Gs and nanobody 60 (Nb60), which have not been previously studied in complex with β1 adrenergic receptor (β1AR) by NMR. Complexes with pindolol and Nb60 induce highly similar inactive states of the receptor, closely resembling the resting state conformational ensemble. We show that, upon binding of mini-Gs or nanobody 80 (Nb80), large allosteric changes throughout the receptor take place. The conformation of tβ1AR stabilized by the native-like mini-Gs protein is highly similar to the conformation induced by the currently used surrogate Nb80. Interestingly, in both cases residual dynamics are present, which were not observed in the resting states. Finally, we reproduce a pharmaceutically relevant situation, where an antagonist abolishes the interaction of the receptor with the mini-G protein in a competitive manner, validating the functional integrity of our preparation. The presented system is therefore well suited for reproducing the individual steps of the activation cycle of a G protein-coupled receptor (GPCR) in vitro and serves as a basis for functional and pharmacological characterizations of more native-like systems in the future.
Efficient Synthesis of New Fluorinated β-Amino Acid Enantiomers through Lipase-Catalyzed Hydrolysis
Authors: Shahmohammadi Sayeh , Fülöp Ferenc , Forró Enikő
Published: December 2020

An efficient and novel enzymatic method has been developed for the synthesis of β-fluorophenyl-substituted β-amino acid enantiomers through lipase PSIM (Burkholderia cepasia) catalyzed hydrolysis of racemic β-amino carboxylic ester hydrochloride salts 3a–e in iPr2O at 45 °C in the presence of Et3N and H2O. Adequate analytical methods were developed for the enantio-separation of racemic β-amino carboxylic ester hydrochlorides 3a–e and β-amino acids 2a–e. Preparative-scale resolutions furnished unreacted amino esters (R)-4a–e and product amino acids (S)-5a–e with excellent ee values (≥99%) and good chemical yields (>48%).
Towards Iron(II) Complexes with Octahedral Geometry: Synthesis, Structure and Photophysical Properties
Authors: Darari Mohamed , Francés-Monerris Antonio , Marekha Bogdan , Doudouh Abdelatif , Wenger Emmanuel , Monari Antonio , Haacke Stefan , Gros Philippe C.
Published: December 2020

The control of ligand-field splitting in iron (II) complexes is critical to slow down the metal-to-ligand charge transfer (MLCT)-excited states deactivation pathways. The gap between the metal-centered states is maximal when the coordination sphere of the complex approaches an ideal octahedral geometry. Two new iron(II) complexes (C1 and C2), prepared from pyridylNHC and pyridylquinoline type ligands, respectively, have a near-perfect octahedral coordination of the metal. The photophysics of the complexes have been further investigated by means of ultrafast spectroscopy and TD-DFT modeling. For C1, it is shown that—despite the geometrical improvement—the excited state deactivation is faster than for the parent pseudo-octahedral C0 complex. This unexpected result is due to the increased ligand flexibility in C1 that lowers the energetic barrier for the relaxation of 3MLCT into the 3MC state. For C2, the effect of the increased ligand field is not strong enough to close the prominent deactivation channel into the metal-centered quintet state, as for other Fe-polypyridine complexes.
Eco-Friendly 1,3-Dipolar Cycloaddition Reactions on Graphene Quantum Dots in Natural Deep Eutectic Solvent
Authors: Giofrè Salvatore , Tiecco Matteo , Celesti Consuelo , Patanè Salvatore , Triolo Claudia , Gulino Antonino , Spitaleri Luca , Scalese Silvia , Scuderi Mario , Iannazzo Daniela
Published: December 2020

Due to their outstanding physicochemical properties, the next generation of the graphene family—graphene quantum dots (GQDs)—are at the cutting edge of nanotechnology development. GQDs generally possess many hydrophilic functionalities which allow their dispersibility in water but, on the other hand, could interfere with reactions that are mainly performed in organic solvents, as for cycloaddition reactions. We investigated the 1,3-dipolar cycloaddition (1,3-DCA) reactions of the C-ethoxycarbonyl N-methyl nitrone 1a and the newly synthesized C-diethoxyphosphorylpropilidene N-benzyl nitrone 1b with the surface of GQDs, affording the isoxazolidine cycloadducts isox-GQDs 2a and isox-GQDs 2b. Reactions were performed in mild and eco-friendly conditions, through the use of a natural deep eutectic solvent (NADES), free of chloride or any metal ions in its composition, and formed by the zwitterionic trimethylglycine as the -bond acceptor, and glycolic acid as the hydrogen-bond donor. The results reported in this study have for the first time proved the possibility of performing cycloaddition reactions directly to the p-cloud of the GQDs surface. The use of DES for the cycloaddition reactions on GQDs, other than to improve the solubility of reactants, has been shown to bring additional advantages because of the great affinity of these green solvents with aromatic systems.
Biofilm Formation as a Complex Result of Virulence and Adaptive Responses of Helicobacter pylori
Authors: Krzyżek Paweł , Grande Rossella , Migdał Paweł , Paluch Emil , Gościniak Grażyna
Published: December 2020

Helicobacter pylori is a bacterium that is capable of colonizing a host for many years, often for a lifetime. The survival in the gastric environment is enabled by the production of numerous virulence factors conditioning adhesion to the mucosa surface, acquisition of nutrients, and neutralization of the immune system activity. It is increasingly recognized, however, that the adaptive mechanisms of H. pylori in the stomach may also be linked to the ability of this pathogen to form biofilms. Initially, biofilms produced by H. pylori were strongly associated by scientists with water distribution systems and considered as a survival mechanism outside the host and a source of fecal-oral infections. In the course of the last 20 years, however, this trend has changed and now the most attention is focused on the biomedical aspect of this structure and its potential contribution to the therapeutic difficulties of H. pylori. Taking into account this fact, the aim of the current review is to discuss the phenomenon of H. pylori biofilm formation and present this mechanism as a resultant of the virulence and adaptive responses of H. pylori, including morphological transformation, membrane vesicles secretion, matrix production, efflux pump activity, and intermicrobial communication. These mechanisms will be considered in the context of transcriptomic and proteomic changes in H. pylori biofilms and their modulating effect on the development of this complex structure.