bluuebunny/crossref_metadata_2025 huggingface dataset:
RNA structural probing of guanine and uracil nucleotides in yeast
Authors: Xiao Kevin , Ghalei Homa , Khoshnevis Sohail
Published: July 2023

RNA structure can be essential for its cellular function. Therefore, methods to investigate the structure of RNA in vivo are of great importance for understanding the role of cellular RNAs. RNA structure probing is an indirect method to asess the three-dimensional structure of RNA by analyzing the reactivity of different nucleotides to chemical modifications. Dimethyl sulfate (DMS) is a well-established compound that reports on base pairing context of adenine (A) and cytidine (C) in-vitro and in-vivo, but is not reactive to guanine (G) or uracil (U). Recently, new compounds were used to modify Gs and Us in plant, bacteria, and human cells. To complement the scope of RNA structural probing by chemical modifications in the model organism yeast, we analyze the effectiveness of guanine modification by the glyoxal family in Saccharomyces cerevisiae and Candida albicans. We show that within glyoxal family of compounds, phenylglyoxal (PGO) is the best guanine probe for structural probing in S. cerevisiae and C. albicans. Further, we show that PGO treatment does not affect the processing of different RNA species in the cell and is not toxic for the cells under the conditions we have established for RNA structural probing. We also explore the effectiveness of uracil modification by Cyclohexyl-3-(2-Morpholinoethyl) Carbodiimide metho-p-Toluenesulfonate (CMCT) in vivo and demonstrate that uracils can be modified by CMCT in S. cerevisiae in vivo. Our results provide the conditions for in vivo probing the reactivity of guanine and uracil nucleotides in RNA structures in yeast and offer a valuable tool for studying RNA structure and function in two widely used yeast model systems.
Improved GWO and its application in parameter optimization of Elman neural network
Authors: Liu Wei , Sun Jiayang , Liu Guangwei , Fu Saiou , Liu Mengyuan , Zhu Yixin , Gao Qi
Published: July 2023

Traditional neural networks used gradient descent methods to train the network structure, which cannot handle complex optimization problems. We proposed an improved grey wolf optimizer (SGWO) to explore a better network structure. GWO was improved by using circle population initialization, information interaction mechanism and adaptive position update to enhance the search performance of the algorithm. SGWO was applied to optimize Elman network structure, and a new prediction method (SGWO-Elman) was proposed. The convergence of SGWO was analyzed by mathematical theory, and the optimization ability of SGWO and the prediction performance of SGWO-Elman were examined using comparative experiments. The results show: (1) the global convergence probability of SGWO was 1, and its process was a finite homogeneous Markov chain with an absorption state; (2) SGWO not only has better optimization performance when solving complex functions of different dimensions, but also when applied to Elman for parameter optimization, SGWO can significantly optimize the network structure and SGWO-Elman has accurate prediction performance.
Altered growth and death in dilution-based viral predation assays
Authors: Knowles Ben , Bonachela Juan A. , Cieslik Nick , Della Penna Alice , Diaz Ben , Baetge Nick , Behrenfeld Micheal J. , Naumovitz Karen , Boss Emmanuel , Graff Jason R. , Halsey Kimberly H. , Haramaty Liti , Karp-Boss Lee , Bidle Kay D.
Published: July 2023

Viral lysis of phytoplankton is one of the most common forms of death on Earth. Building on an assay used extensively to assess rates of phytoplankton loss to predation by grazers, lysis rates are increasingly quantified through dilution-based techniques. In this approach, dilution of viruses and hosts are expected to reduce infection rates and thus increase host net growth rates (i.e., accumulation rates). The difference between diluted and undiluted host growth rates is interpreted as a measurable proxy for the rate of viral lytic death. These assays are usually conducted in volumes ≥ 1 L. To increase throughput, we implemented a miniaturized, high-throughput, high-replication, flow cytometric microplate dilution assay to measure viral lysis in environmental samples sourced from a suburban pond and the North Atlantic Ocean. The most notable outcome we observed was a decline in phytoplankton densities that was exacerbated by dilution, instead of the increased growth rates expected from lowered virus-phytoplankton encounters. We sought to explain this counterintuitive outcome using theoretical, environmental, and experimental analyses. Our study shows that, while die-offs could be partly explained by a ‘plate effect’ due to small incubation volumes and cells adhering to walls, the declines in phytoplankton densities are not volume-dependent. Rather, they are driven by many density- and physiology-dependent effects of dilution on predation pressure, nutrient limitation, and growth, all of which violate the original assumptions of dilution assays. As these effects are volume-independent, these processes likely occur in all dilution assays that our analyses show to be remarkably sensitive to dilution-altered phytoplankton growth and insensitive to actual predation pressure. Incorporating altered growth as well as predation, we present a logical framework that categorizes locations by the relative dominance of these mechanisms, with general applicability to dilution-based assays.
Vaccine hesitancy among healthcare workers in low- and middle-income countries during the COVID-19 pandemic: Results from facility surveys across six countries
Authors: Baral Prativa , Ahmed Tashrik , Amor Fernandez Pablo , Peters Michael A. , Drouard Salome Henriette Paulette , Muhoza Pierre , Mwinnyaa George , Mwansambo Charles , Nzelu Charles , Tassembedo Mahamadi , Uddin Md. Helal , Wesseh Chea Sanford , Yansane Mohamed Lamine , Bergeron Julie Ruel , Karibwami Alain-Desire , Lopez Chicheri Tania Inmaculada Ortiz de Zuniga , Ogunlayi Munirat Iyabode Ayoka , Sieleunou Isidore , Hashemi Tawab , Hansen Peter M. , Shapira Gil
Published: July 2023

Background

Vaccine hesitancy remains a critical barrier in mitigating the effects of the ongoing COVID-19 pandemic. The willingness of health care workers (HCWs) to be vaccinated, and, in turn, recommend the COVID-19 vaccine for their patient population is an important strategy. This study aims to understand the uptake of COVID-19 vaccines and the reasoning for vaccine hesitancy among facility-based health care workers (HCWs) in LMICs.

Methods

We conducted nationally representative phone-based rapid-cycle surveys across facilities in six LMICs to better understand COVID-19 vaccine hesitancy. We gathered data on vaccine uptake among facility managers, their perceptions of vaccine uptake and hesitancy among the HCWs operating in their facilities, and their perception of vaccine hesitancy among the patient population served by the facility.

Results

1,148 unique public health facilities participated in the study, with vaccines being almost universally offered to facility-based respondents across five out of six countries. Among facility respondents who have been offered the vaccine, more than 9 in 10 survey respondents had already been vaccinated at the time of data collection. Vaccine uptake among other HCWs at the facility was similarly high. Over 90% of facilities in Bangladesh, Liberia, Malawi, and Nigeria reported that all or most staff had already received the COVID-19 vaccine when the survey was conducted. Concerns about side effects predominantly drive vaccine hesitancy in both HCWs and the patient population.

Conclusion

Our findings indicate that the opportunity to get vaccinated in participating public facilities is almost universal. We find vaccine hesitancy among facility-based HCWs, as reported by respondents, to be very low. This suggests that a potentially effective effort to increase vaccine uptake equitably would be to channel promotional activities through health facilities and HCWs.However, reasons for hesitancy, even if limited, are far from uniform across countries, highlighting the need for audience-specific messaging.
Persistence and stability of generalized ribosome flow models with time-varying transition rates
Authors: Vághy Mihály A. , Szederkényi Gábor
Published: July 2023

In this paper some important qualitative dynamical properties of generalized ribosome flow models are studied. Ribosome flow models known from the literature are generalized by allowing an arbitrary directed network structure between compartments, and by assuming general time-varying rate functions corresponding to the transitions. Persistence of the dynamics is shown using the chemical reaction network (CRN) representation of the system where the state variables correspond to ribosome density and the amount of free space in the compartments. The L1 contractivity of solutions is also proved in the case of periodic reaction rates having the same period. Further we prove the stability of different compartmental structures including strongly connected ones with entropy-like logarithmic Lyapunov functions through embedding the model into a weakly reversible CRN with time-varying reaction rates in a reduced state space. Moreover, it is shown that different Lyapunov functions may be assigned to the same model depending on the non-unique factorization of the reaction rates. The results are illustrated through several examples with biological meaning including the classical ribosome flow model on a ring.
SUNi mutagenesis: Scalable and uniform nicking for efficient generation of variant libraries
Authors: Mighell Taylor L. , Toledano Ignasi , Lehner Ben
Published: July 2023

Multiplexed assays of variant effects (MAVEs) have made possible the functional assessment of all possible mutations to genes and regulatory sequences. A core pillar of the approach is generation of variant libraries, but current methods are either difficult to scale or not uniform enough to enable MAVEs at the scale of gene families or beyond. We present an improved method called Scalable and Uniform Nicking (SUNi) mutagenesis that combines massive scalability with high uniformity to enable cost-effective MAVEs of gene families and eventually genomes.
Multi-layer laser cutting of electrical steel sheets applied to electric machine laminations
Authors: Dodd Nathan , Ballantyne Erica , Heron Graeme , Goodall Russell
Published: July 2023

With the move away from fossil fuels, the importance of electric machines is increasing. This is particularly the case within major engineering sectors such as the automotive industry. There is therefore a need to further develop processes which will allow for the diverse range of machining operations and large volume manufacture which will be required to overcome the inherent challenges in making this transition. Several critical components of an electric machine, such as the rotor and the stator, are made from electrical grade steel. This is a steel where the composition and processing acts to optimise the magnetic and other properties for the application. The steel is processed as thin sheet laminations and then stacked, to reduce the losses which occur within it due to the generation of eddy currents. The laminations need to be cut to shape, in an operation currently carried out most frequently by stamping from a sheet, but which could be done with greater flexibility by laser cutting (due, for example, to the absence of tooling). In laser cutting the possibility exists to perform cutting operations using what we call here apolystromatamethod, where several sheets are stacked and then cut simultaneously, increasing the efficiency of the operation. To date there have been few reports on this type of laser cutting process, and none that provide detail on the effect that the number of layers in a cutting stack has on critical parameters, such as the edge quality post cutting and the magnetic performance of the sheets. In this work we perform an experimental study of the process and report data in these measures, quantifying the decrease in performance as the stack increases in number of sheets.
Prevalence and factors associated with depression, anxiety, and stress among people with disabilities during COVID-19 pandemic in Bangladesh: A cross-sectional study
Authors: Roy Nitai , Amin Md. Bony , Mamun Mohammed A. , Sarker Bibhuti , Hossain Ekhtear , Aktarujjaman Md.
Published: July 2023

Background

The COVID-19 pandemic has had a profound impact on the mental health of individuals across various populations. People with disabilities (PWDs) are particularly vulnerable to these effects, yet there is a lack of studies investigating the mental health of PWDs in Bangladesh. This study aims to investigate the prevalence of and factors associated with depression, anxiety, and stress among PWDs during the COVID-19 pandemic in Bangladesh.

Methods

Data was collected through interviews with 391 PWDs between December 2020 and February 2021. Demographic information, clinical characteristics, and scores from the Depression, Anxiety, and Stress Scale (DASS-21) were obtained. Chi-square tests and logistic regression analyses were conducted to examine the relationship between psychological measures and potential risk factors.

Results

The prevalence was found to be 65.7% for depression, 78.5% for anxiety, and 61.4% for stress, respectively. Several factors were identified as associated with these mental health issues, including gender (male), marital status (being married), low education levels, multiple impairments, comorbid medical illnesses, poor sleep quality, rural residency, hearing disability, disability onset later in life, and testing positive for COVID-19.

Conclusions

The prevalence was found to be 65.7% for depression, 78.5% for anxiety, and 61.4% for stress, respectively. Several factors were identified as associated with these mental health issues, including gender (male), marital status (being married), low education levels, multiple impairments, comorbid medical illnesses, poor sleep quality, rural residency, hearing disability, disability onset later in life, and testing positive for COVID-19.
Los actores detrás de la política educacional de la dictadura civil militar en Chile (1973-1990)
Authors: Zurita Garrido Felipe , Pérez Navarro Camila
Published: July 2023

El objetivo de este artículo es analizar las trayectorias de los intelectuales que participaron en las distintas instancias encargadas de legislar en torno a la política educativa implementada durante la Dictadura Civil Militar liderada por Augusto Pinochet en Chile (1973-1990). El grupo de intelectuales analizados se encuentra integrado por los Ministros de Educación del periodo 1973-1978, los Capitanes de Fragata Eduardo Cabezón Contreras y Juan Enrique Fröemel Andrade, como así también los integrantes civiles y militares de Comisiones Legislativas vinculadas al campo educacional. En términos metodológicos, se trabajó desde una perspectiva historiográfica organizada en torno al diálogo con una amplia diversidad de fuentes que permitieron acceder al conjunto de actores vinculados al proceso legislativo, como así también permitieron conocer algunas de sus intervenciones. De esta forma, se analizaron leyes y decretos promulgados por la Dictadura Civil Militar, además de las actas de la Junta Militar de Gobierno que registraron el desarrollo de las reuniones de trabajo legislativo llevadas a cabo por los sujetos aquí analizados. Los resultados muestran, por un lado, la relevancia adquirida por intelectuales militares en la primera etapa del régimen, además de la temprana influencia de los Chicago Boys en la producción ideológica sobre educación de la Dictadura Civil Militar chilena.
Persistent Gastrointestinal Symptoms in Human Mpox
Authors: MacDonald Maria , Barker Kim , Umar Imran , Carpentier Stephanie
Published: July 2023

Mpox is a zoonotic disease caused by the double-stranded DNA Mpox virus (MPXV). Little information has been published regarding the gastrointestinal system and MPXV. This case presents a patient with active ileitis and 60 days of functionally limiting diarrhea after confirmation of MPXV. A diagnosis of postinfectious irritable bowel syndrome was made; however, despite a lack of apparent viral shedding on stool polymerase chain reaction, it remains possible that prolonged diarrhea represented direct sequelae from the MPXV disease. This is important from a public health perspective, suggesting that our ability to recommend removal from isolation may need to be reconsidered.
Lolly Willowes: The Berg Collection Manuscript
Authors: Warner Sylvia Townsend
Published: July 2023

The article gives a brief account of the manuscript of Lolly Willowes in the Berg Collection in the New York Public Library and of Warner’s process of revising it for publication. Two extracts from the manuscript follow, the first from the beginning of the novel, and the second from Laura’s dialogue with Satan near the end.
Davy Crockett, apropriação histórica americana e sua interpretação pelos estúdios Disney
Authors: Pegoraro Celbi
Published: July 2023

Este artigo propõe uma análise da apropriação histórica e interpretação de Davy Crockett pelos estúdios Disney, incluindo a minissérie e a representação do personagem em histórias em quadrinhos. Davy Crockett, o renomado "rei da fronteira selvagem", foi introduzido como um proeminente personagem de ação real por Walt Disney na década de 1950. Fundamentado na história dos Estados Unidos, este estudo compreende a lógica histórica e cultural por trás da produção da Disney e explora a intricada relação histórico-artística na criação da minissérie de Davy Crockett e na presença do personagem nas histórias em quadrinhos. Por meio da análise de fontes bibliográficas e documentais, essa pesquisa adentra as complexas dinâmicas que moldam narrativas históricas dentro da cultura popular e indústria cinematográfica. Essa análise oferece perspectivas sobre a complexa relação entre história e mídia na formação da memória coletiva e identidade cultural.
Terror, memória e trauma em À sombra das torres ausentes, de Art Spiegelman
Authors: Mendes Gruner Clóvis
Published: July 2023

O objeto desse artigo é a história em quadrinhos “À sombra das torres ausentes”, de Art Spiegelman. Publicada em forma de livro em 2004, suas 10 pranchas apareceram entre 2002 e 2003, individualmente ou em conjunto, em jornais e revistas dos Estados Unidos e países da Europa. Na primeira parte, é apresentada a obra e sua trajetória no mercado editorial norte-americano, até a edição definitiva. Na sequência, são discutidas algumas das imagens produzidas por Spiegelman a partir da noção de acontecimento sem precedentes, do filósofo francês Jacques Derrida. Finalmente, na terceira e última parte, são analisadas duas pranchas com o intuito de pensar como a narrativa de Spiegelman produz relações com o tempo histórico e a memória traumática. Recorrendo aos conceitos de montagem e remontagem, de Georges Didi-Huberman, a intenção é mostrar como as experimentações visuais de Spiegelman criam uma singular anacronia temporal em que o presente é atravessado pelas distintas temporalidades passadas que o habitam.
Para Além de Wertham
Authors: Cardoso Polatto Rodrigo
Published: July 2023

Este artigo é parte dos resultados obtidos em minha dissertação de mestrado, “O Furor dos Quadrinhos nos EUA do Pós-Guerra, 1940-1954: EC Comics e crítica social”. Defendo no artigo que a importância atribuída à figura controversa do psiquiatra Fredric Wertham, conhecido por associar revistas em quadrinhos à delinquência juvenil, no processo que levou à implementação do código dos quadrinhos é superdimensionada. Argumento que as campanhas antiquadrinhos que levaram ao código foram o resultado de conjunturas históricas específicas e de três fatores principais: (I) o clima doméstico dos EUA gerado pela Guerra Fria que temia a infiltração de ameaças externas; (II) a eclosão de problemas sociais gerados durante a Segunda Guerra Mundial, mas que eclodiram nos primeiros anos da Guerra Fria; (III) as mudanças e desenvolvimentos da indústria de quadrinhos na década do pós-guerra. Concluo que a figura de Wertham e sua teoria foi instrumentalizada por um processo político maior que buscou legitimar as campanhas antiquadrinhos com um verniz de ciência quando em realidade ela foi levada a cabo por diversos grupos diferentes - políticos, religiosos e associações civis - que se opunham às revistas por razões diversas como moralidade, política e elementos estéticos pautados no debate de alta e baixa cultura.
A figuratividade na semiótica discursiva
Authors: Santos Flavia Karla Ribeiro , Portela Jean Cristtus
Published: July 2023

Este artigo visa apresentar a origem do conceito de figuratividade na semiótica discursiva, partindo do princípio de que foi incorporado à metalinguagem dessa disciplina por meio de empréstimo conceitual da teoria estética. Também é objetivo deste trabalho mostrar como o olhar sobre a figuratividade, cujo papel na construção do sentido envolve a articulação de simulacros do mundo natural com as dimensões tímica e sensível conforme os sujeitos percebem e apreendem os objetos para que o enunciatário seja persuadido a crer no discurso manifestado em textos verbais, não verbais e sincréticos, se transforma ao longo dos anos, periodizando as mudanças de perspectiva sobre a figuratividade à medida que é descrita a sua evolução. O corpus é composto de livros e artigos que tratam do papel desse conceito na construção do sentido, publicados entre 1966 e 2008. Resulta dessa investigação a verificação de que a semiótica se apoia sobretudo nos estudos panofskyanos sobre iconografia e iconologia, mas também em contribuições teóricas de linguistas como Hjelmslev e Jakobson. Por fim, a pesquisa conclui que a figuratividade se mantém operacional na semiótica e contribuindo para a evolução da teoria.
A racionalidade neoliberal-conservadora em práticas de desinformação sobre a educação brasileira
Authors: Peixoto Mariana Rafaela Batista Silva
Published: July 2023

Este artigo busca discutir as representações de gênero, sexualidade, raça e classe social em práticas de desinformação sobre a educação brasileira, considerando seu impacto na construção de um imaginário acerca da ciência e educação no país. A partir do enquadramento teórico-metodológico da análise de discurso franco-brasileira e dos estudos feministas interseccionais, são analisados excertos extraídos de peças de desinformação que foram (re)produzidas por agentes do Estado brasileiro ao longo de um período de quatro anos, conforme verificado por agências de checagem de fatos. Os resultados de análise apontam que, enquanto dispositivo de um projeto transnacional neoliberal-conservador, a desinformação parece entretecer o funcionamento de tecnologias nas quais tal projeto se estabelece: nomeadamente, branquitude e cisheteropatriarcado. Consequentemente, por meio de práticas de desinformação sobre educação, propaga-se um ideal utilitarista de educação e ciência no país, contribuindo, assim, para a intensificação de desigualdades e erosão da democracia.
O autor de livros didáticos e o professor de língua materna em paratextos editoriais (1936-1962)
Authors: Imbruniz Cristian Henrique
Published: July 2023

Neste artigo, investigo os modos que a Companhia Editora Nacional encontrou para participar das discussões sobre a formação de professores entre os anos 1930 e 1960, momento em que, no Brasil, se institucionalizavam tanto o ensino secundário quanto o superior. Parto da hipótese de que, ao apresentar autores de livros didáticos de português por meio de recursos gráfico-linguísticos dotados de força ilocucionária, a editora instaurava, também, representações do professor modelar de língua materna. Meu objetivo é, portanto, analisar e descrever os paratextos editoriais destinados à apresentação dos autores dos livros didáticos e, estabelecer, a partir deles, possíveis representações do professor modelar. O material compreende quatro livros que, no período investigado, tiveram destaque no meio educacional: Compêndio de língua portuguesa e Manual de língua portuguesa, de Artur de Almeida Torres; e Português para o ginásio e Português para o colégio, de José Cretella Júnior. Como arcabouço teórico, recorro a uma abordagem discursiva de linha francesa, que reconhece, na produção de sentido no discurso e na história, o caráter de prática social das semioses e, por essa via, a performatividade generalizada da linguagem. A metodologia consiste na descrição dos paratextos seguida da análise dos atos de linguagem realizados por meio deles no curso do debate sobre a formação docente. Os resultados obtidos mostram que, ao apresentar seus autores, a Nacional ora delimitou um conjunto seleto de professores autodidatas, legitimados por instituições de ensino tradicionais e grupos intelectuais; ora reclamou a necessidade de professores licenciados e concursados, legitimados por instâncias oficiais e burocráticas.
Perscrutação ergonômica de manifestações osteomusculares em repartição de recursos administrativos de infrações de trânsito
Authors: Marques Wagner da Silveira , Ribeiro Walcimar dos Santos , Silva Deborah Vilas Boas Pires da , Cesar Luan Aguiar Pontes , Cosme Wallace Souza Guimaraes
Published: July 2023

Baixas por doenças ocupacionais e promoção de mais qualidade de vida aos colaboradores são alvos da ergonomia, que se ocupa das condições de adaptação do trabalho humano, segundo a organização das atividades e função do fim proposto, no intuito de propor normas que visem aprimorar o bem-estar e o desempenho geral. Esta pesquisa teve a proposta de realizar uma análise ergonômica em uma repartição de infrações de trânsito no Estado do Rio de Janeiro, cujo setor em tela foi escolhido pelo fato de nele serem desenvolvidas tarefas que apresentam relevante esforço físico por parte dos servidores. Foi utilizado o questionário nórdico para indicar os membros que acusavam incômodos, dores musculoesqueléticas, e, para análise de postura dos servidores, o método RULA, pois, com a aplicação desse artifício, foi possível identificar a tarefa que tinha deficiência ergonômica. Nessa direção, ações corretivas a serem implementadas foram propostas, as quais proporcionaram melhorias no ambiente de trabalho e apresentaram indícios de diminuição de possíveis doenças ocupacionais.
Análise da eficiência de um instituto federal
Authors: Silveira Nauana Gaivota , Maciel Sula Patricia , Giracca Cesar Nunes , Fries Carlos Ernani
Published: July 2023

A eficiência é um princípio da Constituição Brasileira. Entretanto, mensurá-lo na área da educação superior revela-se um desafio, tendo em vista a subjetividade e complexidade envolvidas. O objetivo desta pesquisa é analisar a eficiência do Instituto Federal de Santa Catarina (IFSC), buscando identificar campus de referência e os principais pontos de ineficiência, proporcionando um processo de melhoria por meio de benchmarking interno.  Para tanto, realizou-se pesquisa predominantemente quantitativa, descritiva, com análise documental de dados secundários da instituição do ano de 2021. Uma vez que há diversos inputs e outputs envolvidos, optou-se pela Análise de Envoltória de Dados (DEA), que tem como base problemas de programação linear (PPL) para determinar a eficiência de unidades produtivas (DMUs) - neste caso, os campi, por meio do Software EMS, associado ao Statistics. Análises de correlação e de componentes principais foram aplicadas aos inputs e outputs disponíveis para identificação das variáveis mais adequadas ao modelo, sendo inputs: Área, Docentes e Matrículas Equivalentes; já os outputs: Concluintes, Projetos de Pesquisa e Projetos de Extensão. Foram identificados como campus de referência os campi Canoinhas, Caçador, São Lourenço do Oeste, Urupema e Xanxerê. Os principais pontos de ineficiência foram Jaraguá do Sul e Itajaí. Identificou também a maior parte dos campi fora da escala ótima. Como medidas gerenciais para elevação do desempenho, sugeriu-se predominantemente a elevação dos outputs projetos de pesquisa e extensão - medida de implementação com maior caráter discricionária da instituição. Destacou-se que não é interesse da instituição e da sociedade reduzir o porte dos campi, sendo importante optar pela elevação dos outputs com a manutenção dos quantitativos de inputs. Esta pesquisa contribui com o conhecimento sobre eficiência nas Instituições de Ensino Superior, mais especificamente nos Institutos Federais, trazendo um estudo de caso que permite análises comparativas em busca da otimização dos recursos e redução das ineficiências.
PERANCANGAN STRATEGI PENGELOLAAN RISIKO PEMANGKU KEPENTINGAN BERBASIS PROSES BISNIS PADA PT X
Authors: Prihartono Budhi , Annasthacia Gracella Audrey Phlycia , Fahlevi Fidruzal
Published: July 2023

PT X merupakan perusahaan tambang pasir di daerah Cimangkok, Sukabumi yang sedang mengalami kesulitan dalam mencapai target produksi. PT X memiliki target produksi pasir sebesar 600-700 m3 per bulan namun rata-rata hasil produksi hanya mencapai 400-500 m3per bulan. Penelitian ini bertujuan untuk mengelola risiko pemangku kepentingan yang menghambat perusahaan dalam mencapai target produksinya. Untuk memperoleh hasil analisis risiko yang lebih holistik, penelitian akan meninjau analisis interaksi risiko dengan integrasi metode Interpretive structure modelling dan matriks MICMAC. Hasil analisis risiko pemangku kepentingan menunjukkan bahwa terdapat empat risiko utama yang masuk ke dalam kategori risiko influent dan perlu dikelola oleh perusahaan, yaitu komunikasi yang kurang efektif antar pekerja, hambatan operasional akibat human error, pekerja tidak melakukan operasi sesuai dengan prosedur, dan kurangnya pekerja dengan kapabilitas dan pengetahuan yang memadai. Risiko-risiko ini berasal dari proses bisnis inti yang berkaitan dengan proses bisnis penambangan pasir. Untuk mengelola risiko pemangku kepentingan PT X, hasil studi mengusulkan 4 strategi, yaitu perbaikan struktur organisasi perusahaan, penyusunan job description, penyusunan standard operating procedure, dan pelatihan serta sertifikasi keterampilan teknis pekerja. Abstract[Title: Strategy Design for Pemangku kepentingan-Risk Management Based on Business Process at PT X] PT X is a sand mining company located in Cimangkok area, Sukabumi, which is experiencing difficulties in achieving production targets. With a target of 600-700 m3, PT X can only manage to reach an average of 400-500 m3 sand production. This study aims to manage pemangku kepentingan risk at PT X. To obtain a more holistic risk analysis result, this study involves risk interaction analysis with the integration of the ISM and MICMAC methods. The result shows that there are four main risks from the influential category that need to be managed by the company: ineffective communication among workers, operational problems caused by human error, workers not performing their works according to procedures, and lack of workers with adequate capabilities and knowledge. This risk comes from core business processes that are related to sand mining. To manage the risk of PT X's stakeholders, the results of the study propose 4 strategies: improvement of the company's organizational structure, development of job description, development of standard operating procedures, and training as well as technical certification for workers.Keywords: core business process; stakeholder risk; risk interaction; risk mitigation; ISM-MICMAC
PENGARUH KUALITAS WEBSITE TERHADAP TINGKAT KEPUASAN PELANGGAN MENGGUNAKAN METODE LINIER REGRESI BERGANDA
Authors: Purwaningsih Ratna , Shobrina Salsa Fadlila , Rumita Rani , Nurlaila Diah , Winata Pidi
Published: July 2023

Penelitian ini bertujuan untuk menguji hubungan antara dimensi-dimensi kualitas website terhadap tingkat kepuasan pelanggan terhadap website Tiket.com. Data diperoleh dengan kuesioner terhadap 141 responden pengguna situs Tiket.com. Uji validitas dan reliabilitas dilakukan untuk menguji konstruk penelitian, uji asumsi klasik dilakukan untuk memastikan persamaan regresi yang didapatkan konsisten dan tidak bias. Pengujian model dilakukan dengan metode analisis regresi linier berganda. Hasil analisis menunjukkan bahwa: desain website, keamanan dan privasi, serta empati memiliki pengaruh yang positif dan signifikan terhadap kepuasan pelanggan, sedangkan, interaktivitas, konten dan informasi, reliabilitas, serta daya tanggap tidak secara signifikan mempengaruhi kepuasan pelanggan. Rekomendasi untuk situs Tiket.com adalah pengembangan website untuk meningkatkan kepuasan pelanggan. Abstract[Analysis the Impact of Websitesite Quality on Customer Satisfaction Level using Multiple Linear Regression Method (Case Study: Tiket.com)] This study examines the relationship between the dimensions of website site quality to customer satisfaction level on the Tiket.com website. Data was collected from 141 respondents who were users of the Tiket.com website. A validity and reliability test was conducted to test the research constructs, and a classic assumption test was also undertaken to ensure that the regression equation obtained was correct, unbiased, and consistent. Multiple linear regression analysis was then carried out to test the model. The analysis of this study indicated that website site design, security, privacy, and empathy have a positive and significant influence on customer satisfaction. While interactivity, content and information, reliability, and responsiveness do not significantly affect customer satisfaction. The recommendation for the website site is website site development to increase customer satisfaction.Keywords: website quality; customer satisfaction; multiple linear regression
Pamięć elementarna i jej granice. Żywioł i szczegóły w prozie Zygmunta Haupta
Authors: Zehnder Christian
Published: July 2023

Rekapitulując główne tendencje hauptologii, autor artykułu pyta ponownie o model pamięci w twórczości Zygmunta Haupta i proponuje rozszerzenie ustalonej „epifanicznej” lektury jego prozy o perspektywę geopoetyki. Na materiale licznych opowiadań autora Pierścienia z papieru udowadnia, jak wybujałe szczegóły utraconego świata galicyjskiego wyrastają za każdym razem z jedynego żywiołu pozornie zapamiętanego jako monolityczny i absolutny (deszcz, śnieg, piasek, trawa, powietrze, kurz itd.). W tym wyłanianiu się detali krystalizuje się rodzaj epifanii, która jest skoncentrowana nie tyle na podmiocie, ile na otoczeniu. Poprzez to uzewnętrznienie pamięci – za sprawą żywiołu – szczegóły uzyskują swój specyficzny status niekonieczności, wyrażającej się w typowych dla tego pisarza enumeracjach. Autor artykułu pyta także o granice tego modelu i znajduje je w „polskim kulturalizmie” (Andrzej Mencwel), który w przyrodzie widzi przede wszystkim „świat pozaludzki” i który Haupt w umiarkowanej, apolitycznej formie zdaje się podzielać. Kulturalistyczna postawa Haupta zostaje dookreślona poprzez porównanie niektórych jego opowiadań amerykańskich z negatywnymi opisami krajobrazu Kalifornii przez Czesława Miłosza i Theodora W. Adorno.
Jak Bolesław Prus skorzystałby z ChatuGPT? Opowieść multimedialna stworzona przy wsparciu sztucznej inteligencji
Authors: Michalak Jonasz , Tonak Karolina , Wojcieszak Daria
Published: July 2023

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Solidarność zwierząt - solidarność ze zwierzętami. Na przykładzie literatury pięknej i nie tylko
Authors: Waksmund Ryszard
Published: July 2023

Więzy solidarności łączą nie tylko ludzi, ale również zwierzęta. Przykładem może być włączony do lektur szkolnych Folwark zwierzęcy Orwella, wyrastający z tradycji bajki Ezopowej. Nie jest to jednak tylko zjawisko znamienne dla fantastyki literackiej, gdyż znajduje poświadczenie w badaniach przyrodników i socjologów (zwierzę jako Inny). Artykuł stanowi przegląd utworów ilustrujących postawę solidarności w świecie zwierzęcym oraz zwierząt z ludźmi, zarówno fantastycznych, jak i realistycznych, dla dzieci i dla dorosłych, które bądź figurują na listach lektur szkolnych, bądź mogłyby się tam znaleźć. Nie tylko pisarzy z kręgu literatury obcojęzycznej, np. bracia Grimm, Kipling, Lagerlȍf, Lofting, Czukowski, Bonsels, Adams, Atkinson, ale również polskiej, jak Dygasiński, Reymont, Brzechwa, Żukrowski. Dotyczy to także wspomnieniowej prozy o dzieciństwie, której wartość dydaktyczna jest nie mniejsza.
Wobec inności, słabości, niższości. Lektura prozy wiejskich przestrzeni
Authors: Czyżak Agnieszka
Published: July 2023

Głównym celem artykułu są rozważania związane ze współczesnymi sposobami odczytywania prozy wiejskich przestrzeni. Tekst zawiera rozpoznania zbudowane na obserwacji powszechnych praktyk dostrzegalnych w dzisiejszych strategiach pisania, czytania i interpretowania kulturowych obrazów o tematyce ludowej. Ważne w opisie tych zjawisk okazują się także kategorie spacjalne wypracowane w ramach geopoetyki. Takie perspektywy oglądu kulturowych rozpoznań i przybliżeń chłopskiej kondycji określane jako „zwrot ludowy” lub „zwrot plebejski” pozwalają na odnajdywanie odmiennych strategii czytania utworów eksplorujących wspólne dziedzictwo oraz odkrywanie nowych form solidarności z wykluczonymi. Interpretacje utworów pisanych przez autorów różnych pokoleń, m.in.: Wiesława Myśliwskiego, Mariana Pilota, Annę Janko, Andrzeja Stasiuka, Wiolettę Grzegorzewską, Macieja Płazę mogą posłużyć jako przykłady przemian zachodzących w tradycyjnych sposobach dookreślania podstaw polskiej zbiorowej tożsamości.
Virtual high-throughput screening: Potential inhibitors targeting aminopeptidase N (CD13) and PIKfyve for SARS-CoV-2
Authors: Ruan Zijing , Tang Jiaxi , Zeng Mingtang , Fan Ping
Published: July 2023

Since the outbreak of the novel coronavirus nearly 3 years ago, the world’s public health has been under constant threat. At the same time, people’s travel and social interaction have also been greatly affected. The study focused on the potential host targets of SARS-CoV-2, CD13, and PIKfyve, which may be involved in viral infection and the viral/cell membrane fusion stage of SARS-CoV-2 in humans. In this study, electronic virtual high-throughput screening for CD13 and PIKfyve was conducted using Food and Drug Administration-approved compounds in ZINC database. The results showed that dihydroergotamine, Saquinavir, Olysio, Raltegravir, and Ecteinascidin had inhibitory effects on CD13. Dihydroergotamine, Sitagliptin, Olysio, Grazoprevir, and Saquinavir could inhibit PIKfyve. After 50 ns of molecular dynamics simulation, seven compounds showed stability at the active site of the target protein. Hydrogen bonds and van der Waals forces were formed with target proteins. At the same time, the seven compounds showed good binding free energy after binding to the target proteins, providing potential drug candidates for the treatment and prevention of SARS-CoV-2 and SARS-CoV-2 variants.
Clown doctors virtualized: hospital professionals’ perception regarding online visits during confinement in Portuguese public hospitals
Authors: Peceguina Inês , Alcântara Iêda , Santos Anabela C. , Diab Flávia , Carvalho Sílvia
Published: July 2023

Clown doctors’ artistic interventions directed to hospitalized children in the Portuguese public hospitals have been performed over the last 20 years, uninterruptedly. During the Covid-19 pandemic, the doctor clowns’ visits were interrupted, but soon after resumed in some hospitals, by means of visits online. The present study evaluates hospital professionals’ perception (n = 152) regarding clowns’ online visits. Participants were mostly nurses (n = 76), and child educators/teachers (n = 39) working in pediatrics (n = 139). Six months after the beginning of the visits, an online questionnaire was used to collect the data. A mixed-methods approach was used to analyse the data. Results indicate that most hospital professionals have a positive perception of online visits, considering that the quality of performance was preserved, and that children were engaged and benefited from the interactions. Moreover, participants acknowledge the organizations’ ability and promptness in developing an alternative way of visiting the children, even though some stressed that in-person visits are the most desirable format.
Using social robots for language learning: are we there yet?
Authors: Huang Guanyu , Moore Roger K.
Published: July 2023

Along with the development of speech and language technologies and growing market interest, social robots have attracted more academic and commercial attention in recent decades. Their multimodal embodiment offers a broad range of possibilities, which have gained importance in the education sector. It has also led to a new technology-based field of language education: robot-assisted language learning (RALL). RALL has developed rapidly in second language learning, especially driven by the need to compensate for the shortage of first-language tutors. There are many implementation cases and studies of social robots, from early government-led attempts in Japan and South Korea to increasing research interests in Europe and worldwide. Compared with RALL used for English as a foreign language (EFL), however, there are fewer studies on applying RALL for teaching Chinese as a foreign language (CFL). One potential reason is that RALL is not well-known in the CFL field. This scope review paper attempts to fill this gap by addressing the balance between classroom implementation and research frontiers of social robots. The review first introduces the technical tool used in RALL, namely the social robot, at a high level. It then presents a historical overview of the real-life implementation of social robots in language classrooms in East Asia and Europe. It then provides a summary of the evaluation of RALL from the perspectives of L2 learners, teachers and technology developers. The overall goal of this paper is to gain insights into RALL’s potential and challenges and identify a rich set of open research questions for applying RALL to CFL. It is hoped that the review may inform interdisciplinary analysis and practice for scientific research and front-line teaching in future.
Uncovering enzymatic structural adaptations from energy dissipation
Authors: Arango-Restrepo Andrés , Barragán Daniel , Rubi J. Miguel
Published: July 2023

While genetic mutations, natural selection and environmental pressures are well-known drivers of enzyme evolution, we show that their structural adaptations are significantly influenced by energy dissipation. Enzymes use chemical energy to do work, which results in a loss of free energy due to the irreversible nature of the process. By assuming that the catalytic process occurs along a potential barrier, we describe the kinetics of the conversion of enzyme-substrate complexes to enzyme-product complexes and calculate the energy dissipation. We show that the behaviour of the dissipated energy is a non-monotonic function of the energy of the intermediate state. This finding supports our main result that enzyme configurations evolve to minimise energy dissipation and simultaneously improve kinetic and thermodynamic efficiencies. Our study provides a novel insight into the complex process of enzyme evolution and highlights the crucial role of energy dissipation in shaping structural adaptations.
Utilising commercial farm grass growth data to evaluate long-term dry matter production of perennial ryegrass varieties
Authors: Hearn C. , Egan M. , Lynch M.B. , Tubritt T. , O’Leary M. , Geoghegan A. , O’Donovan M.
Published: July 2023

Little data are currently available on either the dry matter (DM) production of perennial ryegrass (PRG) swards as they age beyond 5 yr (i.e. permanent pasture) or the performance of PRG varieties on commercial grassland farms. Previous work has generally assumed a connection between DM production and ground score (GS) and this link has been used as a proxy for variety persistence. The evolution of technology in the form of PastureBase Ireland has led to agronomic data of individual paddocks being made available for analysis over multiple years which has allowed the long-term assessment of varieties sown as monocultures on commercial farms. This technology allowed for the inclusion of eight PRG varieties sown in 649 paddocks across 101 farms in Ireland in the current analysis. The results show little association between GS and variety DM production as varieties age to 7 yr. Dry matter production of 1- to 4-yr-old swards appeared to provide a strong indication of variety DM production in years 5–7 post-sowing (r = 0.72, P < 0.05). The interaction of variety and sward age was not associated with DM production. Generally, varieties which produced the most DM in younger swards also produced the most DM in permanent pasture swards. Over longer-term periods these variety differences can manifest into large differences in DM produced and consumed on farm. The current analysis suggests that the long-term production benefits of utilising improved PRG varieties in pasture reseeding may be underestimated.
Regulation of the Ca2+Channel CaV1.2 Supports Spatial Memory and Its Flexibility and LTD
Authors: Ireton Kyle E. , Xing Xiaoming , Kim Karam , Weiner Justin C. , Jacobi Ariel A. , Grover Aarushi , Foote Molly , Ota Yusuke , Berman Robert , Hanks Timothy , Hell Johannes W.
Published: July 2023

Widespread release of norepinephrine (NE) throughout the forebrain fosters learning and memory via adrenergic receptor (AR) signaling, but the molecular mechanisms are largely unknown. The β2AR and its downstream effectors, the trimeric stimulatory Gs-protein, adenylyl cyclase (AC), and the cAMP-dependent protein kinase A (PKA), form a unique signaling complex with the L-type Ca2+channel (LTCC) CaV1.2. Phosphorylation of CaV1.2 by PKA on Ser1928is required for the upregulation of Ca2+influx on β2AR stimulation and long-term potentiation induced by prolonged theta-tetanus (PTT-LTP) but not LTP induced by two 1-s-long 100-Hz tetani. However, the function of Ser1928phosphorylationin vivois unknown. Here, we show that S1928A knock-in (KI) mice of both sexes, which lack PTT-LTP, express deficiencies during initial consolidation of spatial memory. Especially striking is the effect of this mutation on cognitive flexibility as tested by reversal learning. Mechanistically, long-term depression (LTD) has been implicated in reversal learning. It is abrogated in male and female S1928A knock-in mice and by β2AR antagonists and peptides that displace β2AR from CaV1.2. This work identifies CaV1.2 as a critical molecular locus that regulates synaptic plasticity, spatial memory and its reversal, and LTD.

SIGNIFICANCE STATEMENTWe show that phosphorylation of the Ca2+channel CaV1.2 on Ser1928is important for consolidation of spatial memory and especially its reversal, and long-term depression (LTD). Identification of Ser1928as critical for LTD and reversal learning supports the model that LTD underlies flexibility of reference memory.