bluuebunny/crossref_metadata_2025 huggingface dataset:
A targeted 3D EM and correlative microscopy method using SEM array tomography
Authors: Burel Agnes , Lavault Marie-Thérèse , Chevalier Clément , Gnaegi Helmut , Prigent Sylvain , Mucciolo Antonio , Dutertre Stéphanie , Humbel Bruno M. , Guillaudeux Thierry , Kolotuev Irina
Published: May 2018

Using electron microscopy to localize rare cellular events or structures in complex tissue is challenging. Correlative light and electron microscopy procedures have been developed to link fluorescent protein expression with ultrastructural resolution. Here, we present an optimized scanning electron microscopy (SEM) workflow for volumetric Array Tomography for asymmetric samples and model organisms (Caenorhabditis elegans, Drosophila melanogaster, Danio rerio). We modified a diamond knife to simplify serial section arrays acquisition with minimal artifacts. After array acquisition, we transfer the arrays to a glass coverslip or silicon wafer support. Using light microscopy, we rapidly screen the arrays for initial recognition of global anatomical features (organs or body traits). Then, using SEM, we perform an in-depth study of the cells and/or organs of interest. Our manual and automatic data acquisition strategies make 3D data acquisition and correlation simpler and more precise than do alternative methods. This method can be used to address questions in cell and developmental biology that require the efficient identification of a labeled cell or organelle.
Predictability of Aggregate Consumption in Brazil: habits, Non-Separability between Consumption and Leisure, or Credit Constraint?
Authors: Barros Júnior Fernando Antônio de , Delalibera Bruno Ricardo , Pinho Neto Valdemar Rodrigues de
Published: May 2018

O objetivo deste trabalho é estudar o consumo agregado no Brasil. Realizamos um teste que permite identificar se o processo de consumo apresenta formação de hábitos, não separabilidade entre consumo e lazer ou se existe um grupo de consumidores com restrição ao crédito. Em nosso exercício empírico encontramos evidências de que os consumidores são restritos e rejeitamos as hipóteses de formação de hábitos e não separabilidade. Desse modo, a renda disponível é um fator importante na determinação do consumo agregado. Além disso, fazemos testes que nos permitem realizar inferência robusta mesmo na presença de instrumentos fracos.
Effectiveness of model-based clustering in analyzing Plasmodium falciparum RNA-seq time-course data
Authors: Oyelade Jelili , Isewon Itunuoluwa , Olaniyan Damilare , Rotimi Solomon O , Soyemi Jumoke
Published: May 2018

Background: The genomics and microarray technology played tremendous roles in the amount of biologically useful information on gene expression of thousands of genes to be simultaneously observed. This required various computational methods of analyzing these amounts of data in order to discover information about gene function and regulatory mechanisms.

Methods: In this research, we investigated the usefulness of hidden markov models (HMM) as a method of clustering Plasmodium falciparum genes that show similar expression patterns. The Baum-Welch algorithm was used to train the dataset to determine the maximum likelihood estimate of the Model parameters. Cluster validation was conducted by performing a likelihood ratio test.

Results: The fitted HMM was able to identify 3 clusters from the dataset and sixteen functional enrichment in the cluster set were found. This method efficiently clustered the genes based on their expression pattern while identifying erythrocyte membrane protein 1 as a prominent and diverse protein in P. falciparum.

Conclusion: The ability of HMM to identify 3 clusters with sixteen functional enrichment from the 2000 genes makes this a useful method in functional cluster analysis for P. falciparum
Phenotype profiling of white-nose syndrome pathogen Pseudogymnoascus destructans and closely-related Pseudogymnoascus pannorum reveals metabolic differences underlying fungal lifestyles
Authors: Chaturvedi Vishnu , DeFiglio Holland , Chaturvedi Sudha
Published: May 2018

Background: Pseudogymnoascus destructans, a psychrophile, causes bat white-nose syndrome (WNS). Pseudogymnoascus pannorum, a closely related fungus, causes human and canine diseases rarely. Both pathogens were reported from the same mines and caves in the United States, but only P. destructans caused WNS. Earlier genome comparisons revealed that P. pannorum contained more deduced proteins with ascribed enzymatic functions than P. destructans.

Methods: We performed metabolic profiling with Biolog PM microarray plates to confirm in silico gene predictions.

Results: P. pannorum utilized 78 of 190 carbon sources (41%), and 41 of 91 nitrogen sources (43%) tested. P. destructans used 23 carbon compounds (12%) and 23 nitrogen compounds (24%). P. destructans exhibited more robust growth on the phosphorous sources and nutrient supplements (83% and 15%, respectively) compared to P. pannorum (27% and 1%, respectively.). P. pannorum exhibited higher tolerance to osmolytes, pH extremes, and a variety of chemical compounds than P. destructans.

Conclusions: An abundance of carbohydrate degradation pathways combined with robust stress tolerance provided clues for the soil distribution of P. pannorum. The limited metabolic profile of P. destructans validated in silico predictions of far fewer proteins and enzymes. P. destructans ability to catabolize diverse phosphorous and nutrient supplements might be critical in the colonization and invasion of bat tissues. The present study of 1,047 different metabolic activities provides a framework for future gene-function investigations of the unique biology of the psychrophilic fungi.
Conic Programming Reformulations of Two-Stage Distributionally Robust Linear Programs over Wasserstein Balls
Authors: Hanasusanto Grani A. , Kuhn Daniel
Published: May 2018

Adaptive robust optimization problems are usually solved approximately by restricting the adaptive decisions to simple parametric decision rules. However, the corresponding approximation error can be substantial. In this paper we show that two-stage robust and distributionally robust linear programs can often be reformulated exactly as conic programs that scale polynomially with the problem dimensions. Specifically, when the ambiguity set constitutes a 2-Wasserstein ball centered at a discrete distribution, the distributionally robust linear program is equivalent to a copositive program (if the problem has complete recourse) or can be approximated arbitrarily closely by a sequence of copositive programs (if the problem has sufficiently expensive recourse). These results directly extend to the classical robust setting and motivate strong tractable approximations of two-stage problems based on semidefinite approximations of the copositive cone. We also demonstrate that the two-stage distributionally robust optimization problem is equivalent to a tractable linear program when the ambiguity set constitutes a 1-Wasserstein ball centered at a discrete distribution and there are no support constraints.

The online appendix is available at https://doi.org/10.1287/opre.2017.1698 .
Lomatuell Pro contact layer and its role in the wound-healing process
Authors: Chamanga Edwin , Jeffrey Steven
Published: May 2018

There is a plethora of wound contact layer dressings on the market each with its own properties to promote healing, which makes dressing selection complicated. An effective and efficient choice of dressing depends on holistic patient assessment, along with an understanding of the wound-healing process, moist wound healing and wound bed preparation. This paper, supported by clinical case studies, demonstrates the effectiveness of the Lomatuell® Pro dressing (Lohmann & Rauscher) in the management of graft wounds, although it is known to be effective in the management of dermal and deep dermal wounds as well. Lomatuell Pro offers benefits of conformability, open mesh gel-forming wound contact properties and a low risk of adhering to the wound bed. It enables moist wound healing by allowing exudate to be absorbed by a secondary dressing. Lomatuell® Pro demonstrates excellence in maintaining a moist wound environment, allows atraumatic dressing removal and encourages a healthy periwound area.
A service evaluation to examine the experience of patients attending wound healing outpatient clinics in South Wales
Authors: Corrin India
Published: May 2018

Chronic wounds are becoming increasingly prevalent in the UK, with a significant proportion of patients being treated in the community. Given the pressures on out-patient services for individuals with wounds, the provision of nurse-led community clinics is increasing. Evaluation of wound services is essential to develop an awareness of where improvements can be made - particularly, although not exclusively, related to nurse-led clinics compared to doctor-led services.

This article describes the development of a patient satisfaction questionnaire to compare the patient experience in two wound clinics located within in South East Wales using a cross sectional survey approach. 117 patients attended these clinics over a 4-week period, and 58 completed the questionnaire. The data showed that most patients in these clinics were over 65 (n=34, 58.6%), with the majority being treated for leg ulcers (n=41, 70.7%). Overall, the feedback was very positive for both clinics, with the majority of patients reporting a high level of satisfaction for both a doctor-led clinic (n=21) and a nurse-led clinic (n=22). However, while the numbers involved in the study were too small to determine any statistical significance and the results only present a snapshot of the situation, it is still clear that patient satisfaction rates between a nurse-led and a doctor-led service are comparable.
Parenting in stepfamilies: revisiting the stepfather’s role
Authors: Atalaia Susana
Published: May 2018

Based on a symbolic interactionist approach, which focuses on family roles negotiation, this article aims to identify the different ways of constructing stepfathering in stepfamily households. Drawing on 30 in-depth interviews with co-resident Portuguese stepfathers, a diversity of patterns was clearly identified. Some roles, patterns or relationships are more involved, individualised and negotiated, whereas others are more distant, mediated and statutory. The article concludes that the way in which stepfathers build their role in a stepfamily relies mostly on the space granted by the mother as well as the stepfather’s willingness to engage in stepfamily life, taking on (or not taking on) tasks and parental responsibilities generally assigned to biological parents. In this sense, the stepfather is a secondary everyday-life parent, who is present and contributes, alongside the mother, to the education and training of the stepchild.
Gum arabic based composite edible coating on green chillies
Authors: Valiathan Sreejit , Athmaselvi K.A.
Published: May 2018

Green chillies were coated with a composite edible coating composed of gum arabic (5%), glycerol (1%), thyme oil (0.5%) and tween 80 (0.05%) to preserve the freshness and quality of green chillies and thus reduce the cost of preservation. In the present work, the chillies were coated with the composite edible coating using the dipping method with three dipping times (1, 3 and 5 min). The physicochemical parameters of the coated and control chillies stored at room temperature (28±2ºC) were evaluated at regular intervals of storage. There was a significant difference (p≤0.05) in the physicochemical properties between the control chillies and coated chillies with 1, 3 and 5 min dipping times. The coated green chillies showed significantly (p≤0.05) lower weight loss, phenolic acid production, capsaicin production and significantly (p≤0.05) higher retention of ascorbic acid, total chlorophyll content, colour, firmness and better organoleptic properties. The composite edible coating of gum arabic and thyme oil with 3 min dipping was effective in preserving the desirable physico-chemical and organoleptic properties of the green chillies up to 12 days, compared to the uncoated chillies that had a shelf life of 6 days at room temperature.
Forecasting daily meteorological time series using ARIMA and regression models
Authors: Murat Małgorzata , Malinowska Iwona , Gos Magdalena , Krzyszczak Jaromir
Published: May 2018

The daily air temperature and precipitation time series recorded between January 1, 1980 and December 31, 2010 in four European sites (Jokioinen, Dikopshof, Lleida and Lublin) from different climatic zones were modeled and forecasted. In our forecasting we used the methods of the Box-Jenkins and Holt- Winters seasonal auto regressive integrated moving-average, the autoregressive integrated moving-average with external regressors in the form of Fourier terms and the time series regression, including trend and seasonality components methodology with R software. It was demonstrated that obtained models are able to capture the dynamics of the time series data and to produce sensible forecasts.
A New Precast Concrete Deck System for Accelerated Bridge Construction
Authors: Morcous George , Hatami Afshin , Jaber Fouad
Published: May 2018

Full-depth precast concrete (PC) deck systems have several advantages over cast-in-place concrete decks in bridge construction, such as improved product quality, reduced construction labor and duration, and less dependence on weather and site conditions. This article presents he development of a new full-depth PC deck system that overcomes the drawbacks of existing systems, which include large numbers of joints and openings that need to be field cast, a requirement for tight tolerances in panel production and erection, a need for an overlay, and a complexity of post-tensioning and grouting operations. The developed deck system consists of full-depth, full-width PC deck panels that are 12 ft (3.66 m) long to minimize the number of transverse joints that need to be field cast. Panels have covered shear pockets at 4-ft (1.22 m) spacing over each girder line. These pockets are designed to minimize the number of shear connectors and deck surface penetrations to eliminate deck overlay. Deck panels are prestressed in both directions: pre-tensioned in the transverse direction and post-tensioned in the longitudinal direction using a new approach that is ductless and easy to install. These unique features are expected to enhance deck constructability, durability, and economy. The article presents the deck system design, construction sequence, and laboratory investigations conducted prior to its implementation in the construction of the Kearney East Bypass bridge in the State of Nebraska, USA.
Generalization of Gough and Pollard Criterion for the Finite Fatigue Life Regime in Proportional Loading Conditions
Authors: Pejkowski Łukasz , Skibicki Dariusz , Kurek Marta
Published: May 2018

In this article, it was shown that for high cycle multiaxial fatigue life prediction, models based only on material parameters in the form of fatigue limits of component loadings should not be used. This is because stress to number of cycles to failure curves of component loadings may be highly nonparallel. The article presents a method of fatigue life prediction including out-of-parallelism of fatigue. In this method, material parameters in the form of fatigue limits were substituted with the α function based on the Basquin equations for tension-compression (or bending) and torsion. The proposal of a new model can be considered as a generalization of the Gough and Pollard criterion. Validation performed using experimental data for ten different materials allowed for fatigue life prediction for multiaxial proportional loading conditions with satisfactory accuracy.
“Why Don't You Speak (Up), Asian/Immigrant/Woman?” Rethinking Silence and Voice through Family Oral History
Authors: Chen Yea-Wen
Published: May 2018

This essay deploys a disruptive moment of giving up tenure to rethink silence and voice in the context of institutional whiteness from the standpoint of a racialized Asian/immigrant/woman faculty. I narrate moments during my first tenure-track years weathering the quiet and invisible storms of whiteness at a historically white institution in the Midwestern United States. In (re)writing my story, I (re)orient my identity as an immigrant Other in US academia, reclaiming my family's oral history to inform my ways of speaking in/with comforting silence. I conclude with a discussion of racialized acts of speaking (up) as an interactive rather than singular moment.
Diversity Thugs
Authors: Hammers Michele , Alexander Bryant Keith
Published: May 2018

This essay explores questions of what it means to participate in publicness—to be both discursively and physically in public spaces and to perform (functionally) and perform (productively) our identities as citizens of local, national, and global communities. Using a multiple/collaborative autoethnographic approach, the authors further theorize on the relationality, emotionality, and affect of both public response and collaborative methodology.
A collaborative and comprehensive approach to women’s musculoskeletal health
Authors: Dualeh Shukri H.A. , Casey Ellen K. , Núñez Ana , Trojan Jeffrey D. , Mulcahey Mary K.
Published: May 2018

This article was migrated. The article was not marked as recommended. Background: The passage of Title IX precipitated a rise in the number of female athletes, an increase in musculoskeletal injuries observed in women, and an understanding that sex disparities in musculoskeletal injuries exist. Women's musculoskeletal health programs have been created to develop sex-specific strategies to prevent and treat musculoskeletal injuries in women. The purpose of this report is to highlight the formative process of a comprehensive Women's Musculoskeletal Health and Sports Medicine Program. Methods: A half-day workshop of research presentations and networking was a preliminary step in the formative process of establishing a Women's Musculoskeletal Health and Sports Medicine Program. This workshop was aimed at facilitating communication and collaboration between subspecialties. Following the workshop, a survey was distributed to assess attendees' impressions of the workshop. Results: Nine of 11 (81.8%) respondents strongly agreed that the workshop was informative and 8 of 11 (72.7%) strongly agreed that they learned something new. Ten of 11 (90.9%) respondents strongly agreed that they would attend a similar workshop in the future. Conclusions: An event focused on defining sex disparity in musculoskeletal injuries, highlighting current research in women's musculoskeletal health, and encouraging networking among health care providers seems to be an effective means of laying the groundwork for the development of a formal comprehensive musculoskeletal health and sports medicine program for women and girls.
We Must Be Dreaming
Authors: Dhert David Bert Joris
Published: May 2018

"One World, One Dream". "For The Game, For The World". "All in One Rhythm." The World Cup and the Olympic Games usually announce themselves in terms of dreams and opportunities for the people of the host country.Along three years of navigating through the daily lives of three Brazilians - one of Indigenous, one of African and one of European descent - the film WE MUST BE DREAMING explores how the 2014 FIFA World Cup and the 2016 Rio Olympic Games have affected the lives of the people of Rio de Janeiro and to what degree the two biggest sport events of the planet have brought the dreams and opportunities they promise.
Immune Checkpoint Inhibitor-Associated Myocarditis
Authors: Ganatra Sarju , Neilan Tomas G.
Published: May 2018

Immune checkpoint inhibitors (ICIs) are approved for a wide range of malignancies. They work by priming the immune system response to cancer and have changed the landscape of available cancer treatments. As anticipated, modulation of the regulatory controls in the immune system with ICIs results in diverse immune-related adverse events, targeting any organ or gland. These toxicities are rarely fatal and generally regress after treatment discontinuation and/or prescription of corticosteroids. Recently, several cases of ICI-related cardiotoxicity have been reported with complications ranging from cardiogenic shock to sudden death. The true incidence of ICI-associated myocarditis is likely underestimated, due to a combination of factors including the lack of specificity in the clinical presentation, the potential of overlap with other cardiovascular and general medical illnesses, the challenges in the diagnosis, and a general lack of awareness of this condition. Currently, there are no clear guidelines for surveillance, diagnosis, or management of this entity. There are multiple unresolved issues including, but not limited to, identifying those at risk of this uncommon toxicity, elucidating the pathophysiology, determining if and what type of surveillance is appropriate, optimal work-up of suspected patients, and methods for resolution of myocarditis. Here we describe a clinical vignette and discuss the salient features and management strategies of ICI-associated myocarditis.

Key Points

The incidence of immune checkpoint inhibitor (ICI)-associated myocarditis is unclear and has been reported to range from 0.06% to 1% of patients prescribed an ICI. Myocarditis may be difficult to diagnose. The risk factors for ICI-associated myocarditis are not well understood but may include underlying autoimmune disease and diabetes mellitus. The prevalence of myocarditis has been reported to be higher with combination immune therapies. Myocarditis with ICI's typically occurs early, with an elevated troponin, may present with an normal left ventricular ejection fraction and may have a fulminant course. The optimal management of myocarditis associated with ICI's is unclear but most cases are treated with high-dose steroids.
The Impact of Tour Guides’ Rapport-building Behavior on Tourists’ Positive Emotions and Subsequent Behaviors
Authors:   The Tourism Sciences Society Of Korea, Park Hae-Mi , Hyun Sung-Hyup
Published: May 2018

This study examined the impact of tour guides' rapport-building behaviors on tourists’ positive emotions and how those emotions influence subsequent behaviors in three specific areas: self-enhancing word-of-mouth, decreased price consciousness, and tolerance to service failure. To do this, 322 online and offline surveys were collected from tourists who had taken guided tours with Korean travel agencies during the past five years. By examining prior studies, the rapport-building behaviors were classified into five factors; uncommonly attentive behavior, common grounding behavior, courteous behavior, connecting behavior, and information-sharing behavior. As a result of the study, uncommonly attentive behavior and courteous behavior positively influenced the tourists’ emotions, while common grounding behavior, connecting behavior, and information-sharing behavior had no effect. This study also confirmed that tourists’ positive emotions had a significant effect on both self-enhancing word-of-mouth, decreased price consciousness of tourists, and tolerance for future service failures.
Positioning of Food Tourism Destinations: Focusing on the Metropolitan Cites
Authors:   The Tourism Sciences Society Of Korea, Chung Eui-Seok , Hwang Jo-Hye
Published: May 2018

As the experience economy through food tourism has increased, local governments have developed various food festivals to encourage local economies through food tourism. However, there is a lack of research to compare the perceptions of food tourism destinations. The purpose of this study is to examine the differences in Seoul residents’ perceptions according to their food involvement and how they perceive the metropolitan cities in Korea as food tourism sites. For this purpose, visualization of the perceptions was accomplished through positioning and surveying the perceptions of the six metropolitan cities. In addition, the differences between the perceptions of the six major metropolitan cities were verified and the perceptions differed among the high and low involvement groups according to food involvement. As a result of the analysis, Busan was found to be higher than the other metropolitan cities in food material recognition, food culture recognition, food recognition, and tourism resource recognition factors. Also, it was found that the group with high food involvement had a higher perception of food tourism sites than the group with low food involvement. This study suggests that government officials and local industries should cooperate to develop tourism products linked to food and to promote local food tourism by encouraging participation and revisitation of people with high food involvement.
The Effects of Self-determination Factors on Customer Participation, Brand Commitment, and Brand Loyalty: Focusing on Customers Using Five-star Hotel Rooms
Authors:   The Tourism Sciences Society Of Korea, Son Young-A , Lee Hyung-Ryong
Published: May 2018

This study was carried out to find out the autonomy, competence, and relatedness, which are self-determined psychological needs, on customer participation, brand commitment, and brand loyalty for customers using five-star hotel rooms in Korea. The survey was conducted from February 1 to February 10, 2018. A total of 353 copies were distributed online, and 301 copies were used for the empirical analysis. For the research analysis, SPSS 22.0 and AMOS 22.0 were used to carry out frequency analysis, descriptive statistical analysis, confirmatory factor analysis, and structural model verification. According to the analyses results, self-determination factors consisted of three factors of autonomy, competence, and relatedness, and they were found to have a positive (+) effect in relation to customer participation. Also, customer participation was found to have a positive (+) effect in relation to consumers' brand commitment and brand loyalty. Finally, brand commitment of customers using five-star hotel rooms was also found to have a positive (+) significant influence relationship with brand loyalty. With new marketing plans to increase the profits of hotel companies, this study provides meaningful implications for the industry and academia.
Barriers Faced in Effective Training Transfer: A Research on Foreign Students
Authors: AYDOĞAN Enver , KHALFAN Khalid Mutasim
Published: May 2018

Abstract 

Training is one of

the frequently used strategy for introducing change, developing human capital

and improving organizational performances. However, the concern over training

initiatives has been and still is the transfer and use of training content to

the work environment as substantiated by literature from disciplines such as

psychology, education and management. The training transfer (TT) environment

where training is to be used is considered one of the most important

determinant of TT as it either facilitate TT or inhibits it. Therefore, the

trainee’s positive perception of the TT climate has the potential to positively

facilitate their transfer of training content to the work environment and vice

versa. The purpose of this research study was to investigate the trainees’

perception of the factors at the TT environment that facilitated or inhibited

(barriers faced) the transfer of Turkish language learnt from Gazi TÖMER to the

relevant environment. In the process, establishing the support that was

received from the various Human Resource actors at the TT environment in

enhancing the general TT outcomes. This was done with the believe that, the

experience gained from this study would be used in bettering TT initiatives in

underdeveloped African countries where training projects for poverty

alleviation and sensitization of communities on development related concerns

have in most cases failed to bear fruits partly due to TT environmental issues.

In a semi-structured interview process, a total of forty former Gazi TÖMER

foreign students who undertook a Turkish language proficiency training between

the year 2014 and 2015 were individually interviewed. The findings suggested

that, the trainees positively perceived the TT environment leading to the

transfer of Turkish language mainly as a result of the support provided by the

various Human Resource actors at the TT environment. Basing on the aims of the

TÖMER training, there was a reported low transfer of academic Turkish mainly

due to training design problem, trainees’ characteristics and language transfer

requiring time to fully be transferred to the environment.
Değişen Devlet Algısı: Toplumsallıktan Bireyselliğe
Authors: ÇELİK Nuriye
Published: May 2018

Post-modernizmle birlikte anlam
kaymasına uğrayan kavramlardan biri devlettir. Eğer bireyler, toplumların
sürekli bir gelişim içerisinde olacağı ve hep daha iyiye gideceği fikrinden
vazgeçmişse, devletin rolü nasıl değişecektir? Kapitalizmin kalesi olan
Amerika’daki gibi bir ekonomik kriz anında, bir baba figürü olarak zorda kalan
tüm ekonomik aktörlere el mi uzatacaktır? Devlet kavramı her gün ve yaşanan her
toplumsal eylem sonrasında tekrar tanımlanmakta ve sorgulanmaktadır. Bu
çalışma, hem ülkemizde hem de dünya genelinde devletin kriz ve korku anlarında
güçlenen ve otoriterleşen; rutin dönemlerde liberalleşip küçülen devinimlerini
açıklamayı hedefler.
The (Re)production of the Industrial Labor Force in Early Republican Turkey: The Case of Sümerbank and Etibank
Authors: DİNGİLOĞLU Selin
Published: May 2018

One of the major problems of the
statist industrialization policies in 1930s and 1940s in Turkey was both the
quantitative and qualitative deficiencies in terms of industrial labor force.
State-run enterprises of Sümerbank and Etibank, which were established in that
period, did not only lead the drive for industrialization, but also for
handling the labor force problems including high rates of worker rotation, lack
of skilled workers, or the issue of peasant-workers, and initiated several
programs and facilities which had crucial consequences as far as the formation
of an industrial working class and the reproduction of industrial labor force
were concerned. The article focuses on three of them -housing, nutrition and
health programs- by examining the evaluations of the bureaucrats and factory
managements as well as the experiences and reactions of the Etibank and
Sümerbank workers.
How to Setup and Perform a qPCR Experiment. v1
Authors: not provided Promega , Wagner Trevor
Published: May 2018

GoTaq® qPCR Master Mix(a,b) is a reagent system for quantitative PCR (qPCR). The system contains a new fluorescent DNA-binding dye that often exhibits greater fluorescence enhancement upon binding to double-stranded DNA (dsDNA) than SYBR. Green I. GoTaq® qPCR Master Mix is provided as a simple-to-use, stabilized 2X formulation that includes all components for qPCR except sample DNA, primers and water. This formulation, which includes a proprietary dsDNA-binding dye, a low level of carboxy-X-rhodamine (CXR) reference dye (identical to ROX™ dye), GoTaq. Hot Start Polymerase, MgCl2, dNTPs and a proprietary reaction buffer, produces optimal results in qPCR experiments. A separate tube of CXR Reference Dye is included for use with instruments that require a higher level of reference dye than that in the GoTaq® qPCR Master Mix. Advantages of the GoTaq® qPCR Master Mix Dye: The proprietary dye provides brighter dsDNA-dependent fluorescence than SYBR. Green I, with less PCR inhibition than SYBR® Green. The dye enables efficient amplification, resulting in earlier quantification cycle (Cq) values and an expanded linear range using the same filters and settings as SYBR® Green I. The CXR reference dye can be detected using the same filters and settings as those used for ROX™ dye. Quantification cycle is formerly known as cycle threshold (Ct). Polymerase/Buffer Formulation: GoTaq® Hot Start Polymerase contains full-length Taq DNA polymerase bound to a proprietary antibody that prevents polymerase activity at room temperature. Thermal activation is achieved by incubating the assembled reaction at 95°C for 2 minutes. The proprietary polymerase/buffer formulation accommodates extended cycle numbers (45–50 cycles) and is compatible with thermal cycling programs that require extended activation (95°C for 10 minutes). Performance: You can expect reliable performance with minimal lot-to-lot variation: efficient, sensitive and linear qPCR amplification over a wide dynamic range. GoTaq® qPCR Master Mix Protocol If you are currently performing dye-based qPCR, the GoTaq® qPCR Master Mix can simply be substituted for your current master mix. For consistency within an experimental set, prepare a sufficient volume of reaction mix without template DNA for the DNA standard reactions and experimental sample reactions. The protocol for a 50μl reaction is outlined below. Component volumes may be scaled as appropriate. This protocol assumes that 20% of the reaction volume is DNA template (e.g., 10μl of DNA template added to 40μl of reaction mix). If the volume of DNA template is more or less than 10μl, adjust the volume of Nuclease-Free Water accordingly so that the final reaction volume is 50μl.
BACRB - Parallel Sequencing of Barcoded BAC Clones by random tagging PCR v1
Authors: Hilario Elena , Davy Marcus , Storey Roy , Newcomb Richard , N Crowhurst Ross , P Hellens Roger
Published: May 2018

A genome is assembled by connecting large scaffolds via a collection of thousands of mate-paired ends of various sizes (3-20 kbp). Terminal ends of long DNA fragments can be obtained by sequencing large insert clones by Sanger sequencing, a labour-intensive and expensive approach. Here we describe a high-throughput protocol to tag 11,520 BAC plasmids randomly with barcoded oligonucleotides at the end of a random hexamer by PCR.This method assisted the kiwifruit (Actinidia chinensis) genome assembly in a similar way as mate paired end datasets. Although not fully sequenced, each randomly tagged BAC clone provides a collection of short sequence tags mapped to a genome across one or multiple scaffolds under three different scenarios: validate a single scaffold, join two scaffolds, or group several scaffolds by proximity only.Our method provides a simple way of cataloging BAC libraries to assist genome assembly projects.
Introduction to Prokaryotic gene prediction (CDS and rRNA) v2
Authors: O Aylward Frank
Published: May 2018

This is a short tutorial on how to get started with gene prediciton in Prokaryotic genomes via the command line. Code is intended for use on an Ubuntu 16.04 LTS OS. The tools we will use here are: Prodigal. https://github.com/hyattpd/Prodigal Barrnap. https://github.com/tseemann/barrnap This tutorial is intended for teaching purposes, but if you use any of the tools in a scientific paper do not forget to cite the appropriate publications! Prodigal: Prodigal: prokaryotic gene recognition and translation initiation site identification. BMC Bioinformatics, 2010, Volume 11, Number 1, Page 1 Doug Hyatt, Gwo-Liang Chen, Philip F LoCascio, Miriam L Land, Frank W Larimer, Loren J Hauser Barrnap: Seemann T (2013), barrnap 0.8 : rap. id ribosomal RNA predictionhttps://github.com/tseemann/barrnap Bedtools BEDTools: a flexible suite of utilities for comparing genomic features. Bioinformatics, Volume 26, Issue 6, 15 March 2010, Pages 841–842, https://doi.org/10.1093/bioinformatics/btq033
CEM (Circular Estimate Method) – Step-by-step Protocol v1
Authors: Josende Marcelo , Manske Nunes Silvana , Müller Larissa , Ferreira Cravo Marlize , Marìa Monserrat José , Ventura Lima Juliane
Published: May 2018

This protocol is part of the entitled paper 'Circular estimate method (CEM) - A simple method to estimate Caenorhabditis elegans culture densities in liquid medium.' DOI: (xxxxxxx), and describes the step-by-step procedures for the achievement of accurate and reliable estimates with the extremely close relationship to the real nematode counting. It can be used on different nematode species and adapted to different laboratory conditions.
Formation of inclusive culture in the implementation of inclusive education: challenges and achievements
Authors: Shemanov A.Y. , Ekushevskaya A.S.
Published: May 2018

The article analyzes the concept of inclusive culture and the methodology of its definition and formation, attempts to apply this methodology in different countries (USA, UK, Spain, New Zealand, Australia). In international practice, educational inclusion is created on the basis of educational organization inclusive culture which is considered as the ground of inclusive policy and practice. In the framework of organizational approach, inclusive culture is defined as a set of shared values which favour creating of inclusive community in organization. Changes of shared values accepting diversity of learners and their educational needs have a key significance in this process. These changes should concern not only declared values, but basic assumptions of educational organization members. The understanding of social justice and equity which lays in the ground of inclusive values influences inclusive policy and practice. In the modern discourse of inclusion, this understanding is based mainly on social-constructionist approach, which conditions preferences in interpretation of social justice and equity in access to education and associated with these preferences risks. In conclusion, the need for the balance between tasks of education and inclusion is suggested in order to minimize risks associated with different understandings of equity in the access to education
Intellectual Disability and Autism Spectrum Disorders
Authors: Sorokin A.B.
Published: May 2018

The article introduces the modern understanding of intellectual disability as a diagnostic category. It is based on the description of the structure, recommended for professional use in the USA. The necessity of intellect testing in individuals with autism spectrum disorders is discussed alongside with its place among other diagnostic measures
A review of psychological studies on social interactions of students in inclusive classrooms
Authors: Konokotin A.V.
Published: May 2018

The article presents a review of current foreign publications on the social interactions of children in inclusive classrooms. It analyzes the studies carried out during the period from 2008 to 2017, i.e. over the past decade. The results show that social interactions of students in inclusive classrooms are often studied in small groups where students with special educational needs and their normally developing peers are gathered together, but researchers ignore other factors that may have an impact on the quality of their interactions. Thus interactions of students are not equal in nature and are not purposefully organized so that researchers are not able to fully identify the role of each student
Niña con síndrome de Down e hipertensión pulmonar
Authors: Corona Villalobos Carlos , López Corella Eduardo
Published: May 2018

La niña llegó a nuestro Instituto a los 11 meses de edad, referida por dificultad respiratoria y con diagnósticos de síndrome de Down, comunicación interatrial (CIA) tipo ostium secundum y estenosis subglótica. Producto del segundo embarazo, de padres de 25 (madre) y 27 (padre) años. En la historia familiar se refiere síndrome de Down en una tía materna. La paciente nació por cesárea a las 38 semanas por oligohidramnios y sufrimiento fetal, pesó 1560 g, con talla de 41 cm, Apgar de 7/8, sin esfuerzo respiratorio al nacer, hospitalizada 45 días, de los cuales 7 requirió ventilación mecánica. Exhibía un fenotipo Down con facies característica, macroglosia y pliegue palmar único, bilateral, con polidactilia en la mano izquierda. Se auscultó un soplo cardiaco y en el ecocardiograma se detectó una comunicación interatrial de 8 mm, con flujo de izquierda a derecha. Egresó con diagnósticos de síndrome de Down, comunicación interatrial, estenosis pulmonar leve,hipertensión arterial pulmonar moderada y displasia broncopulmonardependiente de oxígeno.
Higiene respiratoria. Etiqueta de tos
Authors: Castañeda Narváez José Luis , Hernández Orozco Hilda , Arias de la Garza Eduardo , Ortega Martínez Esther
Published: May 2018

Las enfermedades respiratorias son causa importante de atención en los centros hospitalarios y en la consulta externa. En México, el Sistema Nacional de Vigilancia Epidemiológica de la Secretaría de Salud reportó 1,724,742 casos de infección de vías respiratorias en enero de 2017 y 1,866,660 en enero de 2018.De estas enfermedades, la influenza fue la que dio importancia al uso de la estrategia “Etiqueta de tos”, que consiste en dar a conocer y aplicar las medidas generales que deben tomar familiares, acompañantes, visitantes y todo el personal de salud de la institución para evitar la trasmisión de infecciones respiratorias.
Guia Cabedelo: visualização de dados para o mapeamento social e colaborativo das informações culturais e turísticas da cidade de Cabedelo
Authors: Medeiros Rodrigo Pessoa , De Souza Brito Sanyo Damião , Patrício Rayane Do Nascimento , Almeida de Macedo Júnior Fernando Antônio , De Morais Júnior Elson Ferreira
Published: May 2018

This article aims to present the process of creation of Cabedelo Guide project, a visualization of data that is premised upon the idea of generating of a social mapping in a collaborative way based on tourist and cultural information of the city of Cabedelo. Data visualization tools were used as a visual representation to find patterns in large volumes of data. This type of collaborative project has become popular because it gives the user the opportunity to be a creator and participant of the work. As a methodological process in design, we try to apply the user-centered design process to the creation of data visualization. The process was successful in this project and it indicates that this methodology may be used in other similar projects
ARTE E CONTRASSEXUALIDADE: a performance sadomasoquista sob as interpelações queer e (pós) feminista
Authors: Gadelha José Juliano Barbosa
Published: May 2018

O artigo discorre sobre os sadismo e masoquismo típicos das culturas BDSM (Bondage, Disciplina/Dominação, Submissão/Sadismo, Masoquismo) praticado como performance art em espaços que vão além dos territórios de arte (museus, galerias etc). O autor tem por interpeladores epistemológicos a Teoria Queer, os Performance Studies e algumas vertentes do (Pós)Feminismo, buscando compreender como os atuais agenciamentos entre arte e contrassexualidade nos desempenhos de alguns agentes performers, brasileiros e norte-americanos, fabricam uma arte queer.

Palavras-chave: Arte, Performance, Sadomasoquismo.

                                                                                    
Evaluation of plasma catestatin levels in patient with coronary slow flow
Authors: TOPAL Dursun , BOYRAZ Bedrettin , AKGÜMÜŞ Alkame , PEKER Tezcan , YILMAZ Mustafa , VATANSEVER AĞCA Fahriye , KANAT Selçuk , AYDIN Ömür , ASLAN Burhan
Published: May 2018

﻿Objectives: Coronary slow flow (CSF) is characterized by delayed opasification of the
coronary arteries in the absence of obstructive coronary disease. Catestatin
has several cardiovasculer actions, in addition to diminihed sympato-adrenal
flow. The study was to investigated associations between CSF and plasma
catestatin levels. Method:. This study included 45
CSF patients (37 male, mean age 48 ± 9.5 years) and 30 control individuals (24
male, mean age 48.4 ± 9 years). Coronary
flow was quantified according to the TIMI (Thrombolysis in Myocardial Infarction)
frame count method for coronary arteries. Serum
catestatin levels taken from blood samples were measured by ELISA method. These parameters were compared between the groups. Results: When
compared with to the control group the serum catestatin levels
was found higher in the CSF group. In addition to this, mean platelet volume
was also significantly higher in patients with
coronary slow flow. Conclusions: Our study revealed that catestatin levels are increased in
patients with CSF. Coronary slow flow
that increased catecholaminergic sympathetic system activities seem to be among
the reasons of endothelial dysfunction.
Research on Relationship between Financial Inclusion and Economic Growth of Rwanda: Evidence from Commercial Banks with ARDL Approach
Authors: Bigirimana Moïse , Hongyi Xu
Published: May 2018

This study examines the relationship between financial inclusion and economic growth of Rwanda using annual data from 2004 to 2016. We used ARDL as it is a new approach to the problem of testing the existence of a level relationship between a dependent variable and a set of regressors, when it is not known with certainty whether the underlying regressors are trend- or first-difference stationary as developed by Pesaran. The results of our study revealed that there is long-run relationship between financial inclusion and economic growth of Rwanda.
Análise da ansiedade traço competitiva em jovens atletas
Authors: Interdonato Giovanna Carla , Oliveira Arli Ramos de , Luiz Júnior Clovis Correa , Greguol Márcia
Published: May 2018

A ansiedade no contexto esportivo é um dos fatores psicológicos de muita relevância para os atletas, pois suas diferentes formas de manifestações poderão ou não influenciá-los no momento da competição. Assim, este estudo teve o objetivo de verificar os níveis de ansiedade-traço em atletas infanto-juvenis de diferentes modalidades esportivas. Para tanto foram analisados 73 atletas do sexo masculino, com média de idade de 13,2 anos (±1,88) e tempo de prática de 3,43 anos (±1,79), adeptos de basquetebol, futebol, judô, natação e voleibol. O instrumento de avaliação utilizado foi o “Sport CompetitionAnxiety Test (SCAT), desenvolvido por (MARTENS,1977), o qual avalia os níveis de ansiedade-traço competitiva. Os resultados demonstraram que os atletas, apesar de serem jovens, não apresentaram escores elevados de ansiedade-traço. As análises dos dados revelaram ainda que houve diferença estatisticamente significante nos níveis de ansiedade entre as modalidades individuais e coletivas.
Advancements in Annular-Pressure-Buildup Mitigation for Thunder Horse Wells
Authors: Miller Richard A. , Coy Alejandro , Frank Gustavo , Dondale Aaron
Published: May 2018

A wide variety of annular-pressure-buildup (APB) -mitigation techniques has been deployed in the past 2 decades. In the early 2000s, BP focused efforts on the development and implementation of rupture disks, nitrified foam spacers, syntactic-foam modules, and vacuum-insulated tubing (VIT). Initiatives to simplify operations while maintaining well integrity have led to innovative techniques that expand the APB-mitigation toolkit.

BP's Gulf of Mexico (GOM) Thunder Horse drilling team recently pursued three APB-mitigation techniques. One method is to fully cement the annulus, thereby removing the fluid that is subject to thermal expansion in a trapped annulus. A second method uses a qualified port collar to equalize pressure across a casing string. The third method focuses on better acquisition and use of mud pressure/volume/temperature (PVT) data for a more precise prediction of the APB-design loads.

These methods and techniques have led to the removal of syntactic foam from some wells in the Thunder Horse field. The design change reduces installation time and operational complexity during well construction and abandonment.

This paper provides a description and technical details concerning the planning and job execution for the fully cemented annulus and the use of port collars for pressure equalization. It also discusses the motivation behind acquiring PVT data specific to a particular mud system, and provides interpretation of laboratory data. The work may be useful for other operators as they plan and execute wells subject to the potential for APB.