bluuebunny/crossref_metadata_2025 huggingface dataset:
Molecular Typing, Antibiogram and PCR-RFLP Based Detection of Aeromonas hydrophila Complex Isolated from Oreochromis niloticus
Authors: Algammal Abdelazeem M. , Mohamed Mohamed Fathi , Tawfiek Basma A. , Hozzein Wael N. , El Kazzaz Waleed M. , Mabrok Mahmoud
Published: March 2020

Motile Aeromonas septicemia is a common bacterial disease that affects Oreochromis niloticus and causes tremendous economic losses globally. In order to investigate the prevalence, molecular typing, antibiogram and the biodiversity of Aeromonas hydrophila complex, a total of 250 tilapia (Oreochromis niloticus) were collected randomly from 10 private tilapia farms (25 fish/farm) at El-Sharkia Governorate, Egypt. The collected fish were subjected to clinical and bacteriological examinations. The majority of infected fish displayed ulcerative necrosis, exophthalmia, and internal signs of hemorrhagic septicemia. The prevalence of A. hydrophia complex was 13.2%, where the liver was the most predominant affected organ (54.1%). Polymerase chain reaction (PCR) was used to verify the identification of A. hydrophila complex using one set of primers targeting gyrB as well as the detection of virulent genes (aerA, alt, and ahp). All isolates were positive for the gyrB-conserved gene and harbored aerA and alt virulence genes. However, none of those isolates were positive for the ahp gene. The antimicrobial sensitivity was carried out, where the recovered strains were completely sensitive to ciprofloxacin and highly resistant to amoxicillin. All retrieved strains showed the same phenotypic characteristics and were identical based on the restriction fragment length polymorphism (RFLP). Experimentally challenged fish presented a high mortality rate (76.67%) and showed typical signs as in naturally infected ones. In conclusion, the synergism of phenotypic and genotypic characterization is a valuable epidemiological tool for the diagnosis of A. hydrophila complex. RFLP is a fundamental tool for monitoring the biodiversity among all retrieved strains of A. hydrophia.
Unveiling Pharmacological Responses and Potential Targets Insights of Identified Bioactive Constituents of Cuscuta reflexa Roxb. Leaves through In Vivo and In Silico Approaches
Authors: Adnan Md. , Chy Md. Nazim Uddin , Kamal A.T.M. Mostafa , Chowdhury Md. Riad , Islam Md. Shariful , Hossain Md. Amzad , Tareq Abu Montakim , Bhuiyan Md. Imam Hossain , Uddin Md Nasim , Tahamina Afroza , Azad Md Obyedul Kalam , Lim Young Seok , Cho Dong Ha
Published: March 2020

Cuscuta reflexa Roxb. is traditionally used by the indigenous communities of Bangladesh to treat different diseases, such as pain, edema, tumor, jaundice, and skin infections. This study tested neuro-pharmacological, anti-nociceptive, and antidiarrheal activities by in vivo and in silico experiments for the metabolites extracted (methanol) from the leaves of Cuscuta reflexa (MECR). During the anxiolytic evaluation analyzed by elevated plus maze and hole board tests, MECR (200 and 400 mg/kg) exhibited a significant dose-dependent reduction of anxiety-like behavior in mice. Similarly, mice treated with MECR demonstrated a dose-dependent decrease in the time of immobility in both forced swimming and tail suspension tests. In addition, anti-nociceptive activity was assessed by the chemical-induced (acetic acid and formalin) pain models. In both cases, 400 mg/kg was found to be most effective and significantly (p < 0.001) inhibited acetic acid stimulated writhing and formalin-induced licking (pain response) in mice. Furthermore, antidiarrheal efficacy determined by the castor-oil induced diarrheal model manifested an evident inhibition of diarrheal stool frequency. In parallel, previously isolated bioactive compounds were documented based on the biological activities and subjected to in silico studies to correlate with the current pharmacological outcomes. The selected isolated compounds (15) displayed favorable binding affinities to potassium channels, human serotonin receptor, COX-1, COX-2, M3 muscarinic acetylcholine receptor, and 5-HT3 receptor proteins. Additionally, the ADME/T and toxicological properties were justified to unveil their drug-like properties and toxicity level. Overall, Cuscuta reflexa is bioactive and could be a potential source for the development of alternative medicine.
Berberine Hampers Influenza A Replication through Inhibition of MAPK/ERK Pathway
Authors: Botwina Paweł , Owczarek Katarzyna , Rajfur Zenon , Ochman Marek , Urlik Maciej , Nowakowska Maria , Szczubiałka Krzysztof , Pyrc Krzysztof
Published: March 2020

Background: Berberine (BBR) is an isoquinoline alkaloid which exhibits a variety of biological and therapeutic properties, and has been reported by some to block replication of the influenza virus. However, contradictory results have also been presented, and the mechanistic explanation is lacking. Methods: A panel of cell lines (Madin–Darby canine kidney (MDCK), adenocarcinoma human alveolar basal epithelial cells (A549), lung epithelial type I (LET1)) and primary human airway epithelial cells (HAE) susceptible to influenza virus infection were infected with a seasonal influenza A virus in the presence or absence of BBR. Cytotoxicity towards cell lines was measured using XTT assay. The yield of the virus was analyzed using RT-qPCR. To study the molecular mechanism of BBR, confocal microscopy and Western blot analyses of cellular fractions were applied. Results and conclusions: Our results show cell-type-dependent anti-influenza properties of BBR in vitro which suggests that the compound acts on the cell and not the virus. Importantly, BBR hampers influenza replication in primary human airway epithelium 3D cultures that mimic the natural replication site of the virus. Studies show that the influenza A virus upregulates the mitogen-activated protein kinase/extracellular signal-related kinase (MAPK/ERK) pathway and hijacks this pathway for nucleolar export of the viral ribonucleoprotein. Our results suggest that BBR interferes with this process and hampers influenza A replication.
Translation-Independent Roles of RNA Secondary Structures within the Replication Protein Coding Region of Turnip Crinkle Virus
Authors: Sun Rong , Zhang Shaoyan , Zheng Limin , Qu Feng
Published: March 2020

RNA secondary structures play diverse roles in positive-sense (+) RNA virus infections, but those located with the replication protein coding sequence can be difficult to investigate. Structures that regulate the translation of replication proteins pose particular challenges, as their potential involvement in post-translational steps cannot be easily discerned independent of their roles in regulating translation. In the current study, we attempted to overcome these difficulties by providing viral replication proteins in trans. Specifically, we modified the plant-infecting turnip crinkle virus (TCV) into variants that are unable to translate one (p88) or both (p28 and p88) replication proteins, and complemented their replication with the corresponding replication protein(s) produced from separate, non-replicating constructs. This approach permitted us to re-examine the p28/p88 coding region for potential RNA elements needed for TCV replication. We found that, while more than a third of the p88 coding sequence could be deleted without substantially affecting viral RNA levels, two relatively small regions, known as RSE and IRE, were essential for robust accumulation of TCV genomic RNA, but not subgenomic RNAs. In particular, the RSE element, found previously to be required for regulating the translational read-through of p28 stop codon to produce p88, contained sub-elements needed for efficient replication of the TCV genome. Application of this new approach in other viruses could reveal novel RNA secondary structures vital for viral multiplication.
MEDIAÇÃO DE CONFLITOS NA ESCOLA: Pontos e Contrapontos
Authors: Galdino Rita de Cassia Arruda
Published: March 2020

A escola constitui-se em um lócus por excelência, diante de sua heterogeneidade, de uma diversidade de conflitos, principalmente os conflitos de relacionamento. Embora as discussões sobre conflitos escolares não sejam recentes o tema tem sido muito debatido nos últimos anos, envolvendo a sociedade, a escola, professores e alunos, em torno de questões como violência e intolerância sociais, sexuais, de raça e gênero. Nesse artigo salientamos a urgência de discussões e debates sobre a necessidade do desenvolvimento de práticas que possam contribuir para a resolução de conflitos, entendendo a importância de se estabelecer nas unidades escolares uma cultura que valorize e incentive comportamentos e atitudes que contribuam para a resolução dos conflitos nos meios escolares. Para subsidiar a nossa análise recorremos a autores que discutem temáticas como violência, indisciplina e conflitos.
Pelatihan Pembuatan Bata Ringan Kepada Home Industri Batu Bata Konvensional Guna Mendukung Program Pemerintah 1 Juta Rumah Bersubsidi
Authors: Tanjung Denny Akbar , Munte Sirmas
Published: March 2020

Kemajuan teknologi semakin berkembang. Inovasi produk terus dilakukan untuk mendapatkan produk yang efisien, simpel dan terjangkau. Keputusan untuk terus memperbarui keterampilan dengan meninggalkan cara-cara konvensional menuju modern adalah keputusan yang tepat agar usaha terus berjalan dan berkembang. Seperti halnya pengusaha batu bata merah konvensional dimana dari hari ke hari  angka permintaan pasar terus menurun ini diakibatkan oleh keputusan konsumen memilih batu bata ringan dari pada  batu bata merah. Kelebihan-kelebihan yang ditawarkan batu bata ringan ini menjadi alasan konsumen  untuk berpaling. Selain angka penjualan menurun, mahalnya tanah liat karena diperoleh dari luar wilayah desa dan  tingginya biaya produksi karena memakan waktu yang lama dalam  proses memproduksi batu bata merah  membuat pengusaha batu bata merah konvensional semakin menjerit. Keadaan ini mengharuskan pengusaha batu bata merah konvensional beralih memproduksi batu bata ringan. Namun, didalam peralihannya ke batu bata ringan pengusaha menemui  beberapa hambatan dan rintangan. Seperti  belum adanya keterampilan dalam pembuatan batu bata ringan, tidak diperkenalkannya teknologi serta wilayah pemasaran yang masih kecil. Dengan kegiatan pengabdian kepada masyarakat ini kami ingin mendampingi pengusaha batu bata merah konvensional untuk membantu mencari solusi segala masalah yang dihadapi dalam peralihan produknya.  Kegiatan ini dilaksanakan pada home industri Bapak Eka Prayogi dan Bapak Ahmad selama 3 Bulan dan akan  dievaluasi terhadap mutu produk bata ringan yang dihasilkan dan permintaan pasar terhadap batu bata ringan.
Keinginan Berpindah Kerja ditinjau dari Persepsi Dukungan Organisasi Karyawan Toko Matahari Medan Fair
Authors: Lase Menanti , Manurung Yulinda Septiani , Akmal Mukhaira El
Published: March 2020

Tujuan penelitian ini adalah untuk mengetahui hubungan persepsi dukungan organisasi dengan keinginan berpindah kerja. Penelitian dilakukan dengan jumlah responden sebanyak 119 orang karyawan toko Matahari Medan Fair dipilih dengan menggunakan metode purposive sampling. Sebagai pendekatan masalah ini, digunakan acuan teori Azwar dan Arikunto. Data diperoleh dari skala pengukur dukungan organisasi dan keinginan berpindah kerja. Perhitungan dilakukan dengan uji asumsi, terdiri atas uji normalitas dan linieritas hubungan. Data dianalisis dengan korelasi product moment dengan SPSS 19 untuk Windows. Hasil analisis data yang didapat menunjukkan koefisien korelasi -0,509 dengan p 0,000 (p <0,05), hal ini membuktikan adanya hubungan negatif antara persepsi dukungan organisasi dan keinginan berpindah kerja. Persepsi dukungan organisasi adalah salah satu faktor yang dapat mempengaruhi niat berpindah dengan kontribusi (R²) sebesar 25,9 persen dan sisanya sebesar 74,1 persen dipengaruhi oleh faktor lain yang tidak diteliti. Kesimpulannya, hipotesis adanya hubungan yang bersifat negatif antara persepsi dukungan organisasi dan keinginan berpindah kerja diterima.
Perlindungan Hukum dan Hak Waris Istri Kedua dalam Perkawian Poligami Tanpa Izin
Authors: Deni Misda , Asmuni Asmuni , Erwinsyahbana Tengku
Published: March 2020

Polygamy marriage must be done with the consent or permission of the wife so that this creates a new legal problem regarding the position of the second wife, both in terms of rights and obligations, children, assets in the marriage as well as problems in the distribution of inheritance. The purpose of this paper is to determine the legal protection of a second wife to their legal status in polygamy marriage and to find out the rights of wife's inheritance in polygamy marriage according to Islamic fiqh and positive law. This research method is normative juridical with descriptive analysis research type. Legal protection for the second wife to get the rights and recognition must legalize marriage (isbat nikah) in the Religious Court. The second inheritance right of the wife to the polygamy marriage without permission is the right to inheritance with her husband since the marriage took place and all wives have the same rights to the inheritance within 1/8 if they have children and ¼ if they do not have children.
Gerakan Partai Keadilan Sejahtera dalam Menumbuhkan Partisipasi Politik Generasi Millenial Kota Kisaran Timur pada Pemilihan Presiden 2019
Authors: Anandhi Mentari , Prayetno Prayetno
Published: March 2020

This study aims to determine the Young Asahan PKS movement in fostering Millennial political participation. This research uses a qualitative descriptive approach in data collection and analysis. Data collection techniques using a series of methods as interviews, observation, and documentation. This data is processed through data reduction and then organized through data displays that are displayed in tabular form and then drawn conclusions. The results of this study indicate that PKS Muda Asahan plays an active role in fostering millennial political participation in the 2019 PILPRES in Kota Kisaran Timur District. The role can be seen from the programs established to influence millennials to actively participate in voting, monitoring, campaigning, joining interest groups, and communicating with public officials. Some programs that have been formed to attract millennial interest are considered through the 4 millennial generation development segments; (1) Pariotics; (2) Buddy; (3) Fighter; and (4) Star. However, PKS Muda Asahan has not succeeded in forming political discussion on the external sphere. This obstacle occurs due to the stigma of the general public about the proximity of the Young Asahan PKS to the PKS party.
Meta Analisis : Variasi Penelitian Tingkat Sarjana Mahasiswa Akuntansi Universitas Medan Area
Authors: Rizki Atika
Published: March 2020

Tren penelitian akuntansi saat ini tidak lagi terbatas pada bidang keuangan saja, namun masih banyak ditemukan kecenderungan penelitian di bidang akuntansi masih menggunakan pendekatan dan bidang kajian mainstream. Penelitian ini bertujuan untuk memetakan penelitian mahasiswa akuntansi tingkat sarjana di Universitas Medan Area. Penelitian ini menggunakan meta analisis dan teknik analisis konten untuk melihat peta variasi penelitian yang dilakukan oleh mahasiswa selama tahun studi 2019. Hasil penelitian menunjukkan bahwa penelitian akuntansi yang dilakukan oleh mahasiswa cukup bervariasi. Namun, variasi metode penelitian yang digunakan masih cukup rendah.
Vokal Klasik pada Anak Usia 8-10 Tahun di Qinia Music Course Jalan Sisingamangaraja Medan Amplas
Authors: Tiolamrenta Felisya Debora , Sihombing Lamhot Basani , Wiflihani Wiflihani
Published: March 2020

Penelitian ini bertujuan untuk mengetahui latar belakang  berdirinya Qinia Music Course sebagai lembaga musik. Proses  pembelajaran vokal klasik pada anak usia 8-10 tahun di Qinia Music Course. Metode yang digunakan dalam pembelajaran vokal klasik pada anak usia 8-10 tahun di Qinia Music Course. Sarana dan prasarana yang disediakan untuk menunjang pembelajaran vokal klasik pada anak usia 8-10 tahun di Qinia Music Course. Kendala pengajar dan siswa dalam proses pembelajaran vokal klasik pada anak usia 8-10 tahun di Qinia Music Course. Cara guru mengevaluasi hasil pembelajaran vokal klasik pada anak usia 8-10 tahun di Qinia Music Course. Penelitian ini menggunakan metode penelitian deskriptif yang bersifat kualitatif yaitu suatu metode penelitian yang bertujuan untuk menggambarkan secara rinci dan jelas tentang suatu fenomena yang menjadi fokus penelitian. Sampel dalam penelitian ini adalah Direktur Qinia Music Curse 1 orang, Pengajar/instruktur vokal klsik 1 orang, dan siswa yang berjumlah 10 orang. Pengumpulan data dilakukan melalui observasi, wawancara, demonstrasi, dokumentasi dan studi kepustakaan. Hasil penelitian ini menunjukkan bahwa Metode ceramah, Metode Ceramah Plus Demonstrasi dan Pelatihan, Metode Demonstrasi, Metode Tanya jawab, dan metode latihan (drill) Cukup berhasil membangun pemahaman siswa tentang teori dasar musik, bagaimana membaca notasi dan menyanyikan lagu dengan vokal klasik yang baik.
HIGH PREVALENCE OF MALNUTRITON AND WORM INFECTION AMONG VAN KIEU AND PAKOH CHILDREN AGED 12-36 MONTHS IN DAKRONG DISTRICT, QUANG TRI PROVINCE
Authors: Tran Thi Lan , Le Thi Huong , Nguyen Xuan Ninh
Published: March 2020

Objectives: Assess the nutritional status, worm infection status and some related factors among children aged 12-36 months of Dakrong district, Quang Tri province. Subject and method: A cross sectional study was carried out in 2010, in 680 children aged 12-36 months in 4 communes of Dakrong district, Quang Tri province. Results: The malnutrition rate was 55.0% for underweight, 66.5% for stunting and 16.2% for wasting. The prevalence of malnutrition increases by age group. The prevalence of worm infection was 31.6%, the highest prevalence was belong to Ascaris infection (24.6%), followed by Hookworm and Trichuris (6.5% and 6.2%, respectively). The prevalence of worm infection among children under two is very high (27.0%). The prevalence of worm infection was distributed quite equally between the malnutrition children group and normal children group. Recommendation: Early deworming forchildren from 12 months should be considered as important strategy against the malnutrition of children in Dakrong district, Quang Tri province
QUALITY OF LIFE IN END-STAGE RENAL DISEASE PATIENTS
Authors: Hoang Bui Bao , Le Huu Loi
Published: March 2020

Background: ESRD is severe result from chronic renal diseases. Treatment of ESRDs is still difficult and expensive. Quality of life of these patients is low. Aims: 1. Study quality of life in patients with ESRD by SF-36 questionaire. 2. Estimate the relationship between SF-36 score and clinical, paraclinical features, Kt/V in the patients with ESRD. Patients-Method: cross-sectional study design. 157 ESRD patients in Department of Nephrology and Rheumatology and Department of Hemodialysis – Hue Central Hospital devided into three groups: conservative treatment (CT), hemodialysis (HD) and CAPD. Study quality of life by using SF-36 questionnaire, version 2.0, Vietnamese. Results: 1. Patients with ESRD have average SF-36 score (physical health: 46.75 ± 15.34; mental health: 47.5 ± 14.66; general health: 49.06 ± 14.61). Conservative group has low SF-36 score (physical health: 32.5 ± 15.9; mental health: 29.67 ± 15.29; general health: 32.35 ± 15.52); CAPD group has average SF-36 score (physical health: 59.75 ± 10.91; mental health: 54.43 ± 7.97; general health: 59.21 ± 8.82); HD group has average SF-36 score (physical health: 51.62 ± 11.94; mental health: 54.9 ± 10.31; general health: 55.2 ± 10.49). 2. In conservative health: SF-36 score has negative correlation with serum BUN, creatininemia, dose of erythropoietin, has positive correlation with Hb, HCO3-. In HD group, there are negative correlations between SF-36 score and blood pressure, BUN, creatininemia, positive correlation with Hb, Albuminemia and Kt/V. In CAPD group, negative correlation between SF-36 score and BUN, creatininemia, positive correlation with Hb. Conclusion: Quality of life by SF-36 score in patients with ESRD is at average level and there is negative correlation between SF-36 score and BUN, creatininemia.
NANO-SIZED SOLID DISPERSION OF A POORLY WATER-SOLUBLE DRUG
Authors: Tran Truong Dinh Thao , Tran Ha Lien Phuong , Tran Nghia Khanh , Vo Van Toi
Published: March 2020

Purposes: Aims of this study are dissolution enhancement of a poorly water-soluble drug by nano-sized solid dispersion and investigation of machenism of drug release from the solid dispersion. A drug for osteoporosis treatment was used as the model drug in the study. Methods: melting method was used to prepare the solid dispersion. Drug dissolution rate was investigated at pH 1.2 and pH 6.8. Drug crystallinity was studied using differential scanning calorimetric and powder X-ray diffraction. In addition, droplet size and contact angle of drug were determined to elucidate mechanism of drug release. Results: Drug dissolution from the solid dispersion was significantly increased at pH 1.2 and pH 6.8 as compared to pure drug. Drug crystallinity was changed to partially amorphous. Also dissolution enhancement of drug was due to the improved wettability. The droplet size of drug was in the scale of nano-size when solid dispersion was dispersed in dissolution media. Conclusions: nano-sized solid dispersion in this research was a successful preparation to enhance bioavailability of a poorly water-soluble drug by mechanisms of crystal changes, particle size reduction and increase of wet property.
EVALUATION OF LONGO’S HEMORRHOIDECTOMY AT HUE UNIVERSITY HOSPITAL
Authors: Nguyen Van Lieu , Nguyen Doan Van Phu , Nguyen Thanh Phuc
Published: March 2020

Introduction: Since Longo First described it in 1998, Stapled Hemorrhoidectomy has been emerging as the procedure of choice for symtomatic hemorrhoid. Several studies have shown it to be a safe, effective and relative complication free procedure. The aim of this study was to determine the suitability of (SH) as a day cas procedure at Hue University Hospital.
Methods: From Decembre 2009 to April 2012, 384 patients with third- degree and fourth-degree hemorrhoids who underwent Stapled Hemorrhoidectomy were included in this study. Parameters recorded included postoperative complications, analegic requirements, duration of hospital stay and patient satisfaction. Follow-up was performed at 1 month and 3 months post-operative.
Results: Of the 384 patients that underwent a Stapled Hemorrhoidectomy 252 (65,7%) were male and 132 (34,3%) were female. The mean age was 47,5 years (range 17-76 years. Duration of hospital stay: The mean day was 2,82 ± 1,15 days (range 1-6 days). There were no perioperative complications. There was one case postoperative complication: hemorrhage; Follow-up after surgery: 286 (74,4%) patients had less anal pain, 78 (20,3%) patients had moderate anal pain, 3 (0,8%) patients had urinary retention; Follow-up after one month: good for 325 (84,6%) patients, average for 59 (15,4%) patients; Follow-up after three months: good for 362 (94,3%) patients, average for 22 (5,7%) patients.
Conclusion: Our present study shows that Stapled Hemorrhoidectomy is a safe, reduced postoperative pain, shorter hospital stay and a faster return to unrestricted daily activity
RESEARCH ON NEEDS FOR HIV - INFECTED PEOPLE CARE AT THUA THIEN HUE PROVINCE IN 2011
Authors: Tran Thi Ngoc , Vo Van Thang
Published: March 2020

Objective: Describe the characteristics of people living with HIV / AIDS and survey the needs on take care of physical, mental and society of people infected and the factors related.
Research methods: A cross-sectional survey was conducted among 150 HIV- infected people in Thua Thien Hue Province in 2011.
Results: 80.0% HIV- infected people had need for physical support. Among these cases, there were 89,3% having ARVs treatment need, 86.7% having need for opportunistic infections treatment and 84.0% opportunistic infections prevention treatment respectively. 69.3% of HIV-infected people had mental care need, in which, there were 69.3% asking for participating into regular club activities, 76.0% for being consulted respectively. There were 42% having need for social support in which, there were 36% had need for loaning and 20.7% for vocational training respectively.
Conclusion: 80.0% HIV-infected people had need for physical support, 69.3% of HIV-infected people had mental care need, 42% having need for social support. Keywords: HIV-infected people, HIV, AIDS, physical, mental and social care needs.
PREVALENCE AND THE EXTENT OF TOOTH WEAR AMONG STAFF-WORKERS OF BINH TRI THIEN RAILWAY MANAGEMENT COMPANY IN 2011
Authors: Nguyen Hoang Chung , Vo Van Thang
Published: March 2020

Objective: To determine the prevalence and the extent of tooth wear of Binh Tri Thien Railway Management Company in 2011.
Methodology: This quantitative research, cross-sectional study based on the data from 776 staff-workers whose age range from 18 to 55 years in Binh Tri Thien Railway Management Company, 2011. The severity of tooth wear was clinically evaluated directly according to Tooth Wear Index (TWI) described previously by Smith and Knight (1984). The materials which got at least one tooth surface wear from score-2 is known as tooth wear. The extent of tooth wear is determined by the average number of pathological tooth wear (≥ score-2) on each surface of the tooth. This research examined the relationship between the extent of tooth wear with age, gender, diet, oral hygiene habits and subfunctional activity.
Results: The mean age of the sample was 39.88 ± 10.16. The mean age of men was 40.33 ± 10.06. The mean age of women was 37.51 ± 10.42. Prevalence of tooth wear in this study was 77.7%. Extent of tooth wear: the average number of pathological wear buccal surface: 0.36 ± 1.5, the average number of pathological wear lingual surface: 0.76 ± 2.25, the average number of pathological wear occlusal surfaces/ incisal edges: 8.22 ± 7.99, the average number of pathological wear teeth cervical: 1.98 ± 3.61. Research shows that gender, age, geography, habit of eating sour food, drinking alcohol, drinking carbonated beverage, brushing teeth immediately after eating sour, teeth grinding disability related statistical significantly with extent of tooth wear.
Conclusion: The prevalence of tooth wear in this study was 77.7%. Gender, age, eating sour food habits, drink alcohol, drink carbonated beverages, teeth grinding disability statistically significant relation with the extent of tooth wear.
STUDY ON CLINICAL, PARACLINICAL CHARACTERISTICS AND AEROBIC BACTERIA OF CHRONIC TONSILLITIS AT HUE CENTRAL HOSPITAL AND HUE UNIVERSITY HOSPITAL
Authors: Truong Kim Tri , Nguyen Tu The , Vo Lam Phuoc
Published: March 2020

Objectives: Researching on paraclinical and clinical characteristic, aerobic bacterial and antibiotic graph of chronic tonsilitis.
Materials and methods: 61 tonsilitis patients were studied by crossing, descriptive- statistical and clinic intervention method.
Results: The tonsils appear small sized in 15 and more years old group 83.3%, hypertrophy tonsilitis in 31 and younger years old group 83.6%. Treatment with antibiotics outside hospital 4.9%. White blood cells £10.109/l (88.5%). Positive bacterial rate 65.6%. Bacterial races are often found is hemophilus influenza 27.7%, staphylococcus aureus 17.0%. Antibiotic graph: bacterial sensitize to gentamycin 94.1%, cefalexin 93.8%, vancomycin 91.7%, ciprofloxacin 90.5%, ofloxacin 90.0%, ceftriaxone 89.3%, cefuroxim 83.3%, resist to tetracyclin 46.2%, ampicillin 38.1%, erythromycin 27.3%.
Consclusions: Depending on frequencies bacterial races are often found in chronic tonsilitis afterwards considering carefully designation using antibiotic and bacterial are found in to choose suitable antibiotic.
POSTOPERATIVE PERICARDIAL EFFUSION SYNDROME
Authors: Le Quang Thuu
Published: March 2020

Objective: Today, despite many recent improvements in intraoperative management and postoperative care, late pericardial effusions remain an important cause of morbidity after cardiac surgery. Because of widespread use of chronic anticoagulation and increased complexity of operations, the incidence of effusion may be higher. Thus we need to update the information on the symptoms, risk factors, diagnostic methods and treatment of Postoperative pericardial effusion syndrome.
Patients and methods: A cross-sectional and prospective study of all patients admitted to hospital because of pericardial effusion after open heart surgery from 1/2010 to 9/2012. Study the clinical characteristics, paraclinicals, evaluate the results of treatment of pericardial effusion after open heart surgery.
Results: Symptoms of pericardial effusion are nonspecific. Some patients with pericardial effusion report minimal problems. In the present study, few patients have the classic presentation of tamponade. Echocardiography is the diagnostic accuracy pericardial effusion after open heart surgery. This treatment mainly is pericardial drainage with 100%.
Conclusion: Pericardial effusion is a common complication after open-heart surgery, symptoms of pericardial effusion are nonspecific to diagnostic method is echocardiographic surveillance.patients can be treated with internal medicine if has no tamponade and less fliuds. Pericardial drainage is absolute only in patients with pericardial effusion with signs of cardiac tamponade or pericardial many of effusion.
CLINICAL AND PARACLINICAL CHARACTERISTICS OF PATIENTS INFECTED WITH FASCIOLIASIS
Authors: Dao Trinh Khanh Ly , Tran Van Huy
Published: March 2020

Background and aim: Fascioliasis, which is caused by the large liver fluke species Fasciola hepatica and Fasciola gigantica, is arising and increasing and impacts on the health of people in endemic, specialy in Central and Highland regions of Viet Nam. This study is aimed at assessing the clinical, biological and ultrasound characteristics of human fascioliasis.
Patients and methods: Between 12/2010 and 6/2012, study was carried out on 147 patients infected with fascioliasis at the clinic of IMPE Quy Nhơn. The clinical, biological and ultrasound characteristics were described.
Results: the main clinical manifestations include: abdominal pain accounted for 100% including epigastric pain 84.4%), gastrointestinal disorders accounted for 92,5%, 100% of patients have positive reaction with Fasciola spp. antibody and 100% patients have hepatic lesion on ultrasound with size >30mm accounted on 95,2%. Patients with high eosinophil accounted for 63,9%. Fecal examination to find Fasciola spp. eggs only seen four cases.
Conclusion: the clinical and paraclinical of human fascioliasis has remarkly changed compared with the medical literature.
THE COLOUR VISION DEFICIENCIES AMONG STUDENTS OF HUE SECONDARY SCHOOL OF TRANSPORTATION
Authors: Nguyen Thi Anh Thu , Nguyen Thi Mai Dung
Published: March 2020

Background: Colorblind disability causes difficulties for people in daily life. Derived from the critical requirement to detect the patients in order to help prevent inappropriate careers, especially careers related to transportation, this research aim to determine the situations and the distributions of different types of visual disabilities.
Materials: 1174 students (787 boys and 387 girls) including 2 groups: occupation group and transportation group were tested with ISHIHARA chromatic plates for colour vision deficiencies (CVD) (CVD iclude the total colour blindness, red- green blindness, red-blindness, green- blindness).
The results are showed as follow:
(i) Frequency of CVD boys among boy group is 4.70%;
(ii) Frequency of CVD girls among girls group is 2.58%;
(iii) In boy group, among 3 types of red- green blindness, the green-blindness has the higher frequency (3.18%) comparision with these ones of the red- green blindness and red-blindness. The total colour defiency was hardly;
(iv) Frequency of CVD students among occupation group is 4.15%;
(v) Frequency of CVD students among transportation group is 3.83%.
STUDY OF ANTIOXIDANT EFFECT OF LEAVES XUAN HOA (PSEUDERANTHEMUM PALATIFERUM (NEES) RADLK) ON WHITE MICE (SWISS)
Authors: Doan Suy Nghi
Published: March 2020

Objectives: Researching of antioxidant effects of Pseuderanthemum palatiferum (Nees) Radlk on white swiss.
Subjects and Methods: Pseuderanthemum palatiferum in Hue city and neighbouring (Kim Long, Thuy Xuan, Phu Vang, An Hoa).
Methods: Collection of extracts from Pseuderanthemum palatiferum.
Results:
(i) Research results demonstrate the antioxidant effects of leaves Xuan hoa by experimental research;
(ii) Evaluating the possibility of regulating antioxidant enzyme, catalase, peroxydase, ascobatoxydase of white mice's blood.
Conclusion: Results of research confirmed that the leaf Xuan hoa Pseuderanthemum palatiferum (Nees) Radlk showed antioxidant effect, and regulate the activity of enzymes
SEMEN’S CHARECTERISTICS OF MALES WITH OLYGOSPERMIA AND AZOOSPERMIA
Authors: Tran Duc Phan , Tran Van Sac
Published: March 2020

Objective: There are two reasons could cause male infertility: the first is lack of sperm (including olygospermia and azoospermia), the second is the abnormality in sperm quality or semen quality. The question is: which are the quality of sperm and semen of olygospermia and azoospermia person.
Material and Method: Using case - control method, we analysis 68 semen samples of olygospermia, azoospermia person and 51 semen samples from control person.
Results:
(i) In olygospermia and azoospermia person: the percentage of sperm with normal volume (> 2 ml) was 37.93%, normal pH was 89,6%, normal viscosity was 46.55%. These indicators in control group as follow: 78.43%, 100%, and 84.31%;
(ii) 100% olygospermia person have low rate of sperm mobility and normal sperm morphology.
Discussion: Most of cases with olygospermia and azoospermia have quality abnormaly of semen.
Conclusion: patients with numberal abnormality showed usually quality abnormaly of semen.
HYPERGLYCEMIA IN PATIENTS WITH CRITICAL ILLNESS
Authors: Tran Huu Dang
Published: March 2020

Blood glucose is normally stabled at the level of 70 – 130 mg/dL. Blood glucose is usually changed in case of illness, whether or not having previous diabetes. Hyperglycemia was usually seen in the critical illness patients. Blood glucose is increased in critical illnesses as the increasing in hormone release that make the increase of glucagon, epinephrine, norepinephrine, cortisol, growth hormone and insulin-like growth factor which help to increase blood glucose as well as the increasing in producing inflammatory intermediate factors and cytokines such as TNF-α, IL-1, IL-6. Hyperglycemia result in bad prognostic, nevertheless strict glycemia control causing high risk hypoglycemia. Resonable control of blood glucose in critical illness patients should be less than 8 - 9 mmol/L.

Key words: Blood glucose, critical illness, hyperglycemia due to stress
STUDY ABOUT THE PROGNOSIS VALUE OF HS-CRP, FIBRINOGEN, WHITE BLOOD CELL, BLOOD SEDIMENTATION RATE IN ACUTE STROKE PATIENTS
Authors: Le Chuyen , Le Thi Hang
Published: March 2020

Background: The relationship between the concentration of hs-CRP, fibrinogen, white blood cells, blood sedimentation rate with prognosis in acute stroke patients.
Subjects and Methods: Case-control study, cross-sectional description, over 110 cases including 66 stroke patients (44 cerebral infarction, 22 cerebral hemorrhage) and 44 cases of asthma at Hue University Hospital. Data were processing by conventional statistics methods and SPSS 15.0.
Results: (i) The concentration of hs-CRP, fibrinogen in stroke patients during the acute phase was 6.74±6.61 mg/l and 4.47±0.98 g/l, higher than the control group (p<0.001). WBC was 7729±1549x109/l and VSS (K) 2455±1412 is not different from the control group with p>0.05. There was no difference between 2 groups, cerebral infarction and cerebral hemorrhage; (ii) There was a correlation between inflammatory factors on the severity of stroke patients: Positive correlation between hs-CRP levels with size of brain lesion (p=0.002; r=0.370). Inverse correlation between hs-CRP levels with glasgow scale (p=0.001; r=-0.423), inverse correlation between fibrinogen with size of brain lesion (p=0.043; r=0.250). Inverse correlation between fibrinogen concentration with glasgow scale (p=0.043; r=-0.250).
Conclusion: Serum concentration of hs-CRP, fibrinogen has correlation with the severity in stroke patients during the acute phase.

Key words: acute stroke, hs-CRP, fibrinogen
SUBTYPE A/H7N9 INFLUENZA: PATHOGENICITY AND PREVENTIVE MEASURES
Authors: Tran Dinh Binh
Published: March 2020

H7N9 is a serotype of the species Influenzavirus A. H7 virus normally circulates amongst avian populations with some variants known to occasionally infect humans. A H7N9 virus was first reported to have infected humans in 2013. The people with H7N9 virus are respiratory tract infections leading to pneumonia and can be death. To cope with H7N9 virus should strengthen supervision, strengthening management and treatment, epidemiological investigation and observe who has been exposed to the fatal cases. To limit the risk of disease, WHO recommends that people should be clean, safe eating, wash the hands often, especially before and after eating, after using the toilet, after contact with animals, after contact with sick people, use a mask when in contact with human or environmental exposure to the high-risk disease. Symptoms of influenza H7N9 infection are fever and cough then switch to pneumonia. Therefore, if having the symptoms like this, and then have the cough and shortness of breath, chest pain that should be early come the health facilities to diagnose and treat.
DETERMINATION OF HELICOBACTER PYLORI CAGA GENE AND VACA GENOTYPES IN PATIENTS WITH GASTRIC CANCER
Authors: Le Quy Hung , Ha Thi Minh Thi
Published: March 2020

Background: H. pylori is the first cause of gastric cancer (GC). However, the role of cagA gene and vacA gene in GC is still controversial. This study is aimed at determining the rates of H. pylori infection, cagA gene, vacA genotypes in patients with GC; and evaluating the relationship between cagA gene, vacA genotypes and endoscopic and histopathological features of gastric cancer.
Patients and methods: Fifty eight GC patients and one hundred and sixteen non-GC patients (controls) were enrolled. Infection of H. pylori was determined by PCR. cagA gene and vacA genotypes were determined by Multiplex PCR.
Results: The rate of H. pylori was found in 55.2% in GC group. The rate of cagA gene and vacA gene in GC patients H. pylori positive were found in 78.1% and 100%, respectively. vac A genotypes s1/m1, s1/m2 and s1/m1m2 were found in 34.4%; 50% and 15.6%, respectively. The risk of GC of cagA positive group was higher than cagA negative group, with OR = 4,5; 95%CI = 1.6-12.2. The risk of GC of vacA s1/m1, cagA positive group was higher than vacA s1/m1, cagA negative group, OR = 7.1; 95%CI = 1.4-36. A statistically significative difference of rate of cagA positive was found between Borrmann III/IV group (100%) and Borrmann I/II group (46.2%). A statistically significative difference of rate of cagA positive was found between the tubular adenocarcinoma group (100%) and signet-ring cell carcinoma (44.4%, p = 0,002), and mucinous adenocarcinoma (50%, p =0,024).
Conclusion: Gene cagA and vacA s1/m1 genotype were both risk factors in GC. A significative differences of rate of cagA positive were found between Borrmann groups, and between groups of WHO histopathological classification.

Key words: cagA gene and vacA genotype, Helicobacter pylori, gastric cancer
UPDATE ON THE MANAGEMENT OF IRRITABLE BOWEL SYNDROME
Authors: Tran Van Huy
Published: March 2020

Irritable Bowel syndrome (IBS) is one of the most common diseases in gastrointestinal practice which affect seriously on the quality of life of the patients. A radical and specific therapy is not available until now. Management of IBS needs a complete approach including symptomatic, psychological and physiopathological treatment. Some new agents affecting on the receptors 5-HT3, 5-HT4 and Guanylate Cyclase have recently received approve from FDA and contributed to improve the quality of therapy of IBS. This article aims to review the recent progress in the researches and management of the IBS. Key word: Irritable Bowel syndrome (IBS)
STUDY ON ANTIBIOTIC RESISTANCE OF COMMON PATHOGENIC BACTERIA AT BINH AN HOSPITAL IN KIEN GIANG IN 2010
Authors: Mai Nguyen Ngoc Trac
Published: March 2020

Objective: Study on the distribution of common pathogens at Binh An hospital in 2010 and their antibiotic resistance.
Methods: Retrospective, descriptive and cross-sectional methods were used. Data of bacterial identification and antibiogram results were collected at Binh An hospital from January to December 2010.
Results: The top 5 bacterias were E.coli (33.93%), Streptococcus spp. (23.21%), Staphylococcus aureus (14.29%), Klebsiella pneumoniae (8.93%) and Pseudomonas aeruginosa (7.14%). E.coli strains were high resistant to Ampicillin (100%), Ticarcillin (100%), Trimethoprim/Sulfamethoxazol (85%) and highly sensitive to Imipenem (94%), Cefoperazone/Sulbactam (93%) and Piperacillin/Tazobactam (83%). Resistant rates for Streptococcus spp. were as follows: Oxacillin (100%), Gentamicin (77%), Amikacin (77%), Trimethoprim/Sulfamethoxazol (62%). Streptococcus spp. were sensitive to Vancomycin (100%), Imipenem (100%), Piperacillin and Cefoperazone/Sulbactam (100%). Staphylococcus aureus were high sensitive to Vancomycin (100%) and combinations of Betalactam/Beta-lactamase inhibitor (100%). Carbapenems and combinations of Betalactam/Beta-lactamase inhibitor were effective to Klebsiella spp. Imipenem is still a realistic selection for Pseudomonas aeruginosa
Conclusion: Continuous surveillance of antibiotic resistance as well as reasonable antibiotic use are required to mitigate the progression of antibiotic resistance.

Key words: antibiotic, common pathogens
INTRATHECAL OPIOIDS IN THE INTRA - AND POSTOPERATIVE PAIN MANAGEMENT
Authors: Nguyen Van Minh
Published: March 2020

Opioid analgesics have long been considered as among the most effective treatments for pain, but these medications was used without a fully known mechanism of action until finding its receptor. The discovery of opioid receptors in the dorsal horn has opened a new pain treatment. Intrathecal opioids are widely used as useful adjuncts to anesthesia during regional anesthesia, in the treatment of postoperative acute pain and chronic pain treatment. This article summarizes the historical development and examines the current use of intrathecal opioids in the perioperative period. Pharmacology and clinical use were also reviewed in detail, including dosage in different surgeries, side effects and the treatment.

Key words: opioid, intrathecal analgesia, regional anesthesia
CLINICAL AND PARACLINICAL CHARACTERISTITICS OF ROTAVIRUS DIARRHEA IN CHILDREN LESS THAN 5 YEARS OLD IN PEDIATRIC DEPARTMENT OF BINH DINH GENERAL HOSPITAL
Authors: Nguyen Quoc Tinh , Nguyen Thi Cu
Published: March 2020

Background: Diarrhea is a leading cause of morbidity and mortality in children in developing countries. Rotavirus is the most important factor cause of severe diarrhea and mortality in children, especially under 2 years. Objective: To study Clinical and paraclinical characteristitics of rotavirus diarrhea in children less than 5 years old in the Pediatric Department of Binh Dinh General Hospital. Methods: Cross observational study, 417 children from 2 months to 5 years old who were hospitalized for acute diarrhea without blood in stool in Pediatric Department of Binh Dinh general hospital, from 15/3/2012 to 14/3/2013. There were 228 patients with rotavirus - positive stool specimens and 189 patients with rotavirus - negative stool specimens. Tested for rotavirus A classification by agglutination techniques. Results: Percentage of Rotavirus diarrhea in children <5 years was 54.7%. The mean age of rotavirus diarrhea was 14.83 ± 9.08 tháng months. Ages with the highest cases of rotavirus diarrhea are 2 - 12 months: 48.2%. Rotavirrus diarrhea is experienced in every month but, the highest prevalence is experienced in the spring - summer) with 71,5%. Clinical characteristitics of rotavirus diarrhea: Vomit presents in 91,7% of cases. 32.9% of the initial symptoms of the disease is vomiting. Mean number of vomiting episodes per day: 7 ± 3.57 times, significantly higher compared with non-rotavirus acute diarrhea (p <0,01). Mean number of bowel movement per day: 10.61±4.18 times, mean duration of diarrhea: 6.66 ± 2.52 days. Younger children have higher frequency and duration of diarrhea compared to older children. Paraclinical characteristitics of rotavirus diarrhea: the mean number leukocyte: 9.74 ±3.61 (x 109/l). 2.2% with low blood sodium status; 8.8% with reduction in serum potassium concentration. 26.3% with low blood calcium and glucose status. Laboratory characteristics of stool: 8.8% with white blood cells in stool, 41.7% with carbohydrate in stool. Conclusion: Rotavirus accounting for 54.7% of the causes of diarrhea in children. Rotavirus diarrhea is experienced throughout the year but the highest prevalence is in the spring-summer. Prominent symptoms of the disease are vomiting and watery stool. Children <24 months experience higher frequency and duration of diarrhea compared to older age groups.

Key words: Diarrhea, Rotavirus.
CARDIOVASCULAR RISKS IN OBSTRUCTIVE SLEEP APNEA SYNDROME WITH IMPROVED SASD07 COMPARE TO STARDUST II
Authors: Hoang Anh Tien , Nguyen Thi Y Nhi , Nguyen Luu Trinh , Phan Thi Hong Diep , Nguyen Huu Cat , Nguyen Minh Tam , Doan Phuoc Thuoc , Hoang Khanh , Huynh Van Minh , Cao Ngoc Thanh
Published: March 2020

Background : Sleep Apnea Syndrome (SAS) is a cause of hypertension, increasingcardiovascular risk and cardiovascular disease such as stroke, coronary artery disease, myocardial infarction, arrhythmias, heart failure, increasing the risk of death in patients with heart disease, independent of other causative factors. So far, in Vietnam there are very few studies on Obstructive Sleep Apnea Syndrome (OSAS) and cardiovascular risk factors . Self-making SASD07 is trustly for detecting OSAS with statistical significiant in comparision with StarDustII (gold criteria). Subjects and Methods: Cross sectional study, comparision with control group: 136 peoples (68 in disease group and 68 in control group). Patients were parallelly measured with StarDustII and SASD07 to detect OSAS and find the corellation with cardiovascular risk factors. Results: There is a positive correlation between SBP with the severity of OSAS (r = 0.459, p < 0.001), positive correlation between DBP with the severity of OSAS (r = 0.352, p < 0.003). No statistically significant differences between severe OSAS and fasting blood glucose, cholesterol, HDL Cholesterol, Non - HDL Cholesterol, LDL Cholesterol and TG median (p > 0.05). There is a positive correlation between AHI and neck circumference (r = 0.511, p < 0.001), waist circumference (r = 0.585, p < 0.001), BMI (r = 0.380, p < 0.01). SASD07 diagnostic value of detecting OSAS compared with StarDustII have Kappa = 0.72, (standard error 0.06, p <0.001). Conclusion: The risk factors related to OSAS in our study is neck circumference, waist circumference, systolic blood pressure, diastolic blood pressure. SASD07 have a good value in diagnosing of OSAS compared with polysomnography StarDustII.

Key words: Sleep Apnea Syndrome, cardiovascular risk factor, SASD07.