bluuebunny/crossref_metadata_2025 huggingface dataset:
The southern birch mouse Sicista loriger (Dipodoidea) in the Crimea: distribution, habitats, seasonal ac-tivity, and abundance
Authors:   Crimean Republican Sanitary-Epidemiological Station (Simferopol, Ukraine), Evstafiev Igor
Published: May 2021

The southern birch mouse is a rare and non-abundant species of small mammals of the fauna of the Crimean Peninsula. Its geographic range has gradually reduced during the 20th century. Initially, the southern birch mouse occupied almost the entire territory of the steppe and foothills of the Crimea. As the area of virgin and unploughed lands decreased, the species disappeared from the most part of the peninsula. Whereas the species had been recorded in 11 administrative districts in the middle of the twentieth century, now it is known only in three districts, in two of which it is extremely rare. Currently the southern birch mouse exists in two isolated populations—a western (Tarkhankut) and an eastern (Kerch)—separated by 200 km of anthropogenic landscapes. Census of small mammals has been carried out on trap-lines for the past 40 years. In a total of 667100 traps-nights, 144 birch mice were collected. Additionally, remains of 56 birch mouse specimens were found in 16862 pellets of the long-eared owl. The birch mouse population in the Tarkhankut Peninsula is small (12 specimens were trapped and 39 specimens were identified in pellets), and its range is largely restricted. The species’ population in the Kerch Peninsula is larger (132 birch mice were trapped and 17 specimens were identified in pellets of birds of prey) and its range occupies the entire area of the Kerch Peninsula. Data of long-term epidemiological surveys showed that the ratio of trapped birch mice in the whole of the Crimea is 0.21 %, whereas their ratio in the steppe zone is 0.29 % at a relative abundance of 0.03 specimens per 100 trap-nights. Birch mice are active from mid-April to mid-November. The peak of activity occurs in April, when 49.9 % of animals were trapped. Among natural enemies, the red fox (Vulpes vulpes) can pose a real threat to birch mice, as well as the long-eared owl (Asio otus) to local micropopulations, especially during the breeding season. In our opinion, despite the generally low abundance of birch mice in the Crimea and the fragmentation of its geographic range, extinction does not threatens this species in the peninsula (especially its Kerch population) under the current management system. Conservation of the southern birch mice populations in the Crimea requires a detailed study of the species’ ecology, especially of limiting factors.
Year of the Squirrel 2020: an overview of the topic and events
Authors:   Uzhhorod National University, Zoological Museum (Uzhhorod, Ukraine), Zizda Yulia , Zagorodniuk Igor ,   National Museum of Natural History, NAS of Ukraine (Kyiv, Ukraine), Kharchuk Sergiy ,   Ukrainian Theriological Society, NAS of Ukraine (Kyiv, Ukraine)
Published: May 2021

An overview of information about the squirrel as one of the most notable symbol species is presented. This species is associated with a huge amount of etymological, folklore, historical, symbolic, environmental and research knowledge, initiatives, and research. That is why the squirrel has become one of the symbol species chosen by the Ukrainian Theriological Society in the cycle of annual actions "Years of Mammals". Therefore, this review is largely related to the events devoted to the Year of the Squirrel 2020 in Ukraine. Among the already traditional measures aimed at disseminating knowledge, the cycle "21 most famous facts" is presented: a collection of important information about the squirrel, its biological and behavioural characteristics, and its role in human society. Information on the use of squirrels in symbols—images on coats of arms of different cities, mentions in folklore, the use of images in other areas—is also considered. Information on the colour forms of this rodent is also given, which can be distinguished by acquaintance with squirrels common in Ukraine. Research and projects on squirrels conducted in Ukraine and neighbouring countries, including the preparation of scientific and student works, school projects and photo galleries, are noted separately. Information on the etymology and synonyms of the Ukrainian common name of the squirrel and the areas of their use are presented. The squirrel as a symbol species of 2020 in the activity of the Ukrainian Theriological Society was chosen as a symbol for the logo of the 27th Theriological School organised in Zalishchyky (the school was postponed due to the Covid pandemic). According to the established tradition, a gallery of images of the squirrel sent to the Council of the Society by Ukrainian colleagues was prepared, and the most interesting ones reflecting the plasticity of the species and the variability of its colouration are presented in this review. A photograph of the squirrel is placed on the cover of the current volume of the journal Theriologia Ukrainica (Vol. 20), and the volume itself contains two scientific articles on this species.
The effects of vitamins A, E, D, disorders of their metabolism and the assessment of level of vitamin security in children (literature review)
Authors:   I. Horbachevsky Ternopil National Medical University, Ukraine, Mochulska O.M. , Boyarchuk O.R. ,   I. Horbachevsky Ternopil National Medical University, Ukraine, Kinash M.I. ,   I. Horbachevsky Ternopil National Medical University, Ukraine, Vorontsova T.O. ,   I. Horbachevsky Ternopil National Medical University, Ukraine, Volianska L.A. ,   I. Horbachevsky Ternopil National Medical University, Ukraine
Published: May 2021

Vitamins are a group of biologically highly active, low molecular weight organic compounds of various chemical nature, which are practically not synthesized in the human body or are synthesized in insufficient quantities, mainly income with food, and are vital to ensure for the flow and regulation of metabolic processes in the body. Vitamins are micronutrients that are among the essential (essential) factors of nutrition, their content in food is small, usually in the range from 10 to 100 mg/100 g. Vitamins have an exceptional property — the ability to high biological activity in small doses, without being a source of energy or plastic material, act as biological catalysts for vital body functions. According to physicochemical properties and solubility, vitamins A, E, D belong to fat-soluble, are characterized by thermal stability, resistant to cysts and alkalis, their main characteristic is the ability to promote the assimilation of mаcro- and microelements. Vitamins A, E, D are needed at all stages of growth and development of the child's body, the processes of adaptation. The main types of vitamins metabolism disorders are vitamin deficiency, hypo-, hyper- and dysvitaminosis, polyvitaminosis. It is important to constantly monitor the sufficient content of each vitamins in the daily diet of children. The following methods of assessing vitamin supply are known: clinical, biochemical, calculation of vitamin content. Purpose — to increase knowledgment about the effects of vitamins A, E, D, their importance in metabolism, to analyze the main types of their metabolism disorders, to present possible methods for assessing the supply of vitamins in children. Conclusions. The child's body needs a constant supply of vitamins to maintain their amount at the required level. All vital processes take place in the body during the direct participation of vitamins. Future research in the field of the vitamins A, E, D effects will contribute to a better understanding of their role in ensuring the optimal functioning in a human body. Screening and elimination of vitamin A, E, D deficiency in children can not only improve health, but also help increase the life quality. No conflict of interest was declared by the authors. Key words: vitamins A, E, D, vitamin deficiency, hypovitaminosis, hypervitaminosis, dysvitaminosis, polyvitaminosis, children.
Wykorzystywanie Internetu przez dzieci i młodzież – pozytywne i praktyczne aspekty oraz zagrożenia
Authors:   Mgr, Nauczyciel języka angielskiego, Kłak Ewa
Published: May 2021

The Internet is a tool that enables knowledge and information to spread rapidly, which has a huge impact on the development of person and society. It has a particularly significant impact on the young generation that eagerly use its benefits for the entertainment, communication and information purposes. A mobile phone plays an increasing role here, as it enables continuous, mobile access to the network. However, the effects of using the Internet in everyday life can be positive (e.g. learning) but also negative (e.g. addictions). Research shows that the time spent by young people online is constantly increasing. This causes concern among parents and carers. The use of the Internet by children and adolescents is a current and important topic, that requires constant attention and continuation of research, as well as education of users, parents and teachers on the proper use of the Internet.
Espécies de Meloidogyne em alface na região Agreste do estado de Alagoas
Authors: Assunção Mayara Castro , Noronha Marissônia de Araújo , Muniz Maria de Fátima Silva , Castro José Mauro da Cunha e , Costa João Gomes da
Published: May 2021

RESUMO A meloidoginose da alface interfere negativamente no desenvolvimento da planta, o que reduz a produtividade da cultura. O objetivo desse trabalho foi identificar espécies de Meloidogyne em áreas de cultivo de alface localizadas na região Agreste do estado de Alagoas. Foram realizadas coletas de solo rizosférico e de raízes totalizando 23 amostras, provenientes de sete municípios. As raízes com sintomas de meloidoginose foram cortadas e incorporadas, juntamente com o solo rizosférico, a tomateiros Santa Cruz cv. Kada Gigante, previamente cultivados em vasos contendo solo esterilizado e mantidos sob condições de telado. A identificação das espécies de Meloidogyne foi realizada por meio da análise do perfil da isoenzima esterase. As espécies de M. incognita fenótipos I1 e I2 e de M. javanica fenótipo J3 foram detectadas em 20 e em três áreas, respectivamente.
Modelos basados en variables meteorológicas para estimar el progreso epidémico de la bacteriosis del nogal en variedades de distinto comportamiento
Authors: Seta Silvana Andrea , Moschini Ricardo , Gonzalez Mirian del Pilar
Published: May 2021

RESUMEN La bacteriosis del nogal es causada por Xanthomonas arboricola pv. juglandis. En Zavalla (Santa Fe), la actividad nogalera se centra en la producción de plantas injertadas en vivero. El presente trabajo tuvo por objetivo cuantificar el efecto ambiental sobre el desarrollo epidémico de la bacteriosis del nogal en variedades susceptibles y de mejor comportamiento a la enfermedad y elaborar modelos matemáticos que permitan predecir la severidad de la misma. En la localidad de Zavalla se realizaron observaciones de severidad (Sev%) de la bacteriosis cada siete días en cuatro variedades de nogal (tres susceptibles (sus=1): Chandler, Davis y Tulare y una de mejor comportamiento: Franquette (sus=0)), desde el fin de la primavera hasta los últimos días de febrero (N=76) durante tres campañas (2010/11, 2011/12 y 2012/13). Las variables regresoras meteorológicas se calcularon en los respectivos lapsos de 15 días previos a cada observación de tasa de incremento diario epidémico (Tid%=Sev%t–Sev%t-1/7). Las variables mejor correlacionadas (rk: coeficiente de correlación de Kendall Tau-b) con los niveles de bacteriosis (severo:Tid% >0,1367; moderado a nulo: Tid%<=0,1367, siendo 0,1367 el percentil 40% de los datos observados), para los dos tipos de variedades (sus=1 y sus=0), fueron DPr>9 (días con precipitación>9mm; rk=0,434) y DMojro (días sin lluvia, con mojado atribuible al rocío por ocurrencia de humedad relativa del aire mayor a 82%; rk=0,326). El modelo logístico integrado por DPr>9 y DMojro clasificó correctamente 62 casos de los 76 analizados en las tres campañas (precisión de predicción: PPred=81,5%), permitiendo cuantificar el efecto ambiental en ambos tipos de variedades de nogal analizadas. El modelo integrado por DPr>9 y DMojt (suma los días de mojado por rocío (DMojro) con los originados por precipitación (DMojpr)) logró una precisión de predicción de 80,3%. Ambas variables meteorológicas estarían expresando el requerimiento de agua libre de la bacteria para la infección (DMojt) y de energía en las lluvias (DPr>9) para favorecer la dispersión bacteriana.
Resistência de pimentão ao PepYMV e PepYMV-Lins e obtenção de híbridos resistentes
Authors: Braga Renato , Pavan Marcelo Agenor
Published: May 2021

RESUMO O Pepper yellow mosaic virus (PepYMV) é o mais importante Potyvirus infectando plantas do gênero Capsicum no Brasil. Perdas podem chegar a 100% caso a infecção ocorra no início do cultivo. A resistência genética é a principal forma de controle desta virose. Em 2009 um novo isolado denominado PepYMV-Lins foi detectado quebrando a resistência genética das cultivares comerciais. Este trabalho visou buscar fontes de resistência a este novo isolado, estudar a herança da resistência, incorporar em linhas elites e criar híbridos de pimentões resistentes e com boa performance agronômica. Dentre os acessos do banco de germoplasma da empresa Sakata, foram encontradas seis pimentas e dois pimentões que portavam resistência conjunta aos isolados PepYMV e PepYMV-Lins. Estes dois últimos foram escolhidos para continuar os trabalhos de introdução de resistência. O estudo de herança apontou que a resistência genética nos dois acessos de pimentões é monogênica e recessiva. Eles foram cruzados com as linhagens elites de pimentões com o objetivo de criar híbridos comerciais do tipo cônico. Para acelerar o trabalho de melhoramento foi utilizada a técnica de criação e estabilização de linhagens via duplo haploide. As novas linhagens geradas por esta metodologia foram cruzadas para geração de híbridos. Os novos híbridos mostraram-se resistentes aos isolados PepYMV e PepYMV-Lins. Dois deles (AF23571 e AF23579) foram identificados com estabilidade agronômica e resistência. Testes em larga escala mostraram que o AF23579 foi o que apresentou melhor performance. Este híbrido está disponível comercialmente no mercado brasileiro de sementes de pimentão com o nome de Nocaute.
ASPECTOS HISTÓRICOS DO ENSINO DE LÍNGUA INGLESA NO BRASIL: UMA ANÁLISE DISCURSIVA DO SUJEITO NA PUBLICIDADE AUDIOVISUAL DE CURSOS DE IDIOMAS
Authors: MARQUES Welisson
Published: May 2021

RESUMO Inscrito sob o viés teórico da Análise do Discurso, este artigo apresenta como objetivo geral analisar a constituição do sujeito enunciador no discurso publicitário de cursos de idiomas no Brasil. Nesse sentido, buscaremos analisar como o sujeito significa aprendiz e aprendizagem de língua inglesa nesses espaços enunciativos. Para isso, traremos à discussão, a priori, alguns aspectos históricos que permeiam o ensino de língua inglesa em nosso país. Na sequência, trataremos da base metodológica que constitui a pesquisa ora arrolada nesta reflexão, para enfim, efetuarmos nossas análises. Destacamos, no batimento descrição-interpretação, que os enunciados, de modo regular, evidenciam a importância suprema da habilidade oral. Isso se apresenta na constante mobilização linguística do lexema falar em suas diversas flexões. Outrossim, verifica-se a supremacia do estrangeiro em relação ao brasileiro e, por fim, a exigência que recai sobre o aprendiz brasileiro de língua inglesa em possuir uma pronúncia “perfeita”.
Latent tuberculosis screening before kidney transplantation in the South of Brazil
Authors: Meinerz Gisele , Silva Cynthia Keitel da , Dorsdt Damaris Mikaela Balin , Adames Julia Bertoni , Andrade Julio Pasquali , Ventura Pedro Enrico , Monteiro Alexandre de Almeida , Pasqualotto Alessandro Comarú , Garcia Valter Duro , Keitel Elizete
Published: May 2021

Abstract Background: Tuberculosis (TB) is a prevalent infection after kidney transplantation (KT) in high-burden countries. Latent tuberculosis infection (LTBI) screening includes previous TB history, chest radiograph findings, and tuberculin test (TST) and/or interferon-gamma release assays (IGRAs) results. We aimed to compare our routine LTBI screening of KT candidates and living donors (LD) with their IGRA results, and evaluate if this would improve isoniazid (INH) treatment referral. Methods: We evaluated adult KT candidates and LD with complete routine LTBI screening and QuantiFERON-TB® Gold In-Tube (QFT) testing. Blood samples were collected from April 4th, 2014 to October 31st, 2018, with follow-up until October 31st, 2019. Results: There were 116 KT recipients, with 30% QFT-positive results. Positive QFT was associated with past TB history (p=0.007), positive TST (p<0.0001), residual radiographic lesions (p=0.003), and diabetes (p=0.035). There were 25 LD, 40% had positive QFT. Positive QFT was associated with a positive TST (p=0.002). Positive QFT results increased INH referral in 80%. Post-transplant TB incidence was 2.6% in a median follow-up of 2 (1-33) months. No variables were associated with post-transplant TB. TB patients had inferior, although non-significant, 5-year graft survival (66.7% vs. 76.5%) (p = 0.402). Conclusion: In the present study, the association of QFT to our routine LTBI screening incremented INH treatment referral, but there was still a high incidence of post-transplant TB, possibly related to other forms of infection, such as new exposure and donor transmission.
Autonomia e Práticas Integrativas e Complementares: significados e relações para usuários e profissionais da Atenção Primária à Saúde
Authors: Pereira Léo Fernandes , Rech Cassiano Ricardo , Morini Simone
Published: May 2021

Este estudo investigou os significados de autonomia em saúde e de Práticas Integrativas e Complementares (PIC) atribuídos por usuários e profissionais de um Centro de Saúde (CS) e as possíveis contribuições das PIC para a autonomia do usuário. Foi realizada uma pesquisa qualitativa, cujas informações foram obtidas mediante entrevistas semiestruturadas, gravadas em áudio e interpretadas mediante análise de conteúdo. Participaram 18 indivíduos, entre frequentadores de um grupo de yoga, do serviço de acupuntura e profissionais de um CS de Florianópolis, Santa Catarina, Brasil. Os resultados revelaram entendimentos múltiplos sobre autonomia em saúde e diferenças entre as PIC investigadas, yoga e acupuntura, o que pareceu relacionado à primeira prática estar mais inserida na lógica da promoção da saúde que a segunda, mais restrita ao âmbito da clínica.
Corpo-em-fluxo: conexões entre dança, educação e saúde
Authors: Millás Cláudia Regina Garcia
Published: May 2021

Este artigo trata da relação entre Dança, Educação e Saúde por meio da análise e reflexão de situações que emergiram da atividade docente em um curso de graduação em Dança no âmbito do ensino superior público brasileiro. Faz-se um estudo das experiências obtidas, destacando as concepções e estratégias pedagógicas desenvolvidas nas disciplinas de Dança Acrobática. Constatou-se que as atividades educativas corroboraram para a criação de um espaço de cura enquanto potência de transformação, em que os estudantes puderam se emancipar de uma dada condição e se aproximar de outras perspectivas de vida, sentindo-se em conexão consigo mesmos, com os outros e com o meio. Emprega-se o termo “corpo-em-fluxo” como uma qualidade corporal que o praticante, ao se colocar no momento, íntegro e presente na ação, desfaz-se de uma concepção de si para permitir que outras possam ser criadas.
O diagnóstico tardio na perspectiva do itinerário terapêutico: grau 2 de incapacidade física na hanseníase
Authors: Hespanhol Mirella Chaves Laragnoit , Domingues Sidney Marcel , Uchôa-Figueiredo Lúcia da Rocha
Published: May 2021

Destacam-se casos novos de hanseníase com grau de incapacidade física 2 (GIF 2) que demonstram a ineficiente detecção oportuna. O artigo é um relato de casos e propõe analisar o diagnóstico tardio sob a perspectiva do itinerário terapêutico (IT), com base em um estudo qualitativo. O cenário foram dois municípios da Região Metropolitana da Baixada Santista: Praia Grande e São Vicente. Realizou-se análise documental e entrevista em profundidade com quatro participantes. O material foi submetido à análise de conteúdo e definiram-se as categorias temáticas: cuidado em saúde; corpo na hanseníase; incapacidades na hanseníase; e diagnóstico tardio. Revelaram-se intrincados itinerários terapêuticos, marcados pela fragilidade do cuidado com erro e atraso no diagnóstico, que potencializaram os riscos individuais e coletivos e impactaram negativamente o cotidiano dos sujeitos.
Formação dos profissionais de saúde para o atendimento de LGBTQI+
Authors: Paranhos William Roslindo , Willerding Inara Antunes Vieira , Lapolli Édis Mafra
Published: May 2021

A saúde é um direito de todos. As investigações têm considerado, entre outros aspectos, a qualificação de profissionais da saúde voltada ao atendimento de minorias sociais, incluindo as identidades LGBTQI+ (lésbicas, gays, bissexuais, transexuais, queer, intersexuais, +), na tratativa da integralidade do ser humano. Dessa forma, o estudo busca, por meio de uma revisão sistemática de literatura, identificar as lacunas e potencialidades existentes no processo de formação dos profissionais de saúde no atendimento de LGBTQI+’s. Com abordagem qualitativa, descritiva e exploratória, após a análise verificou-se que profissionais da área da Saúde não estão preparados para atender a essas identidades, tornando-se essencial, para a promoção da saúde e do bem-estar no cerne do atendimento de LGBTQI+, a comunicação por meio de materiais informativos, políticas públicas, reestruturação curricular nos cursos de Saúde, e, ainda, uma desconstrução cultural operando em prol da equidade social.
At-home HIV self-testing during COVID: implementing the GetaKit project in Ottawa
Authors: O’Byrne Patrick , Musten Alexandra , Orser Lauren , Inamdar Gauri , Grayson Marie-Odile , Jones Clay , Francoeur Megan , Lachance Sarah , Paulin Vickie
Published: May 2021

Setting

In March 2020, COVID-19 shuttered access to many healthcare settings offering HIV testing and there is no licensed HIV self-test in Canada.

Intervention

A team of nurses at the University of Ottawa and Ottawa Public Health and staff from the Ontario HIV Treatment Network (OHTN) obtained Health Canada’s Special Access approval on April 23, 2020 to distribute bioLytical’s INSTI HIV self-test in Ottawa; we received REB approval on May 15, 2020. As of July 20, 2020, eligible participants (≥18 years old, HIV-negative, not on PrEP, not in an HIV vaccine trial, living in Ottawa, no bleeding disorders) could register viawww.GetaKit.cato order kits.

Outcomes

In the first 6 weeks, 637 persons completed our eligibility screener; 43.3% (n= 276) were eligible. Of eligible participants, 203 completed a baseline survey and 182 ordered a test. These 203 participants were an average of 31 years old, 72.3% were white, 60.4% were cis-male, and 55% self-identified as gay. Seventy-one percent (n= 144) belonged to a priority group for HIV testing. We have results for 70.9% (n= 129/182) of participants who ordered a kit: none were positive, 104 were negative, 22 were invalid, and 2 “preferred not to say”; 1 participant reported an unreadiness to test.

Implications

Our results show that HIV self-testing is a pandemic-friendly strategy to help ensure access to sexual health services among persons who are good candidates for HIV testing. It is unsurprising that no one tested positive for HIV thus far, given the 0.08% positivity rate for HIV testing in Ottawa. As such, we advocate for scale-up of HIV self-testing in Canada.
ESTABLISHING RELIABILITY AND VALIDITY OF THE ARABIC VERSION OF THE HYPERACUSIS QUESTIONNAIRE
Authors: Alkharabsheh Ana'am , Alaqrabawi Wala
Published: May 2021

Background

To evaluate the extent of hyperacusis, different validated questionnaires have been published in English, such as the Hyperacusis Questionnaire (HQ) developed by Khalfa. However, there is currently no reliable and validated Arabic version of the HQ for use in clinical settings. This study aimed to translate the Khalfa modified HQ into Modern Standard Arabic (MSA) and to determine its validity and reliability in assessing the severity of hyperacusis in Arabic-speaking countries.

Material and methods

The original English version of the Khalfa modified HQ was translated into MSA. The translated version was applied to 142 patients who were divided into two groups: subjects aware of their hyperacusis and subjects not aware of their hyperacusis condition. Internal consistency reliability and Pearson correlation coefficient were calculated. Pairwise comparison of the total score and the score for each dimension was performed using unpaired t-tests. Validity assessment, consisting of Construct and Content validity, were also assessed.

Results

Results show high internal consistency and reliability coefficient. Results of correlation analysis suggest an overall strong and significant correlation between the average score of each item and the overall average score of all items in the questionnaire. The mean difference between the group reporting hyperacusis and the group reporting no hyperacusis was not statistically significant. Also, the gender effect was not statistically significant.

Conclusions

The Arabic version of the modified HQ shows high reliability and validity, suggesting it is a useful tool for the assessment of the severity of hyperacusis of Arabic-speaking patients with hyperacusis.
AUDITORY STREAM SEGREGATION WITH SINUSOIDALLY AMPLITUDE MODULATED TONAL STIMULI IN INDIVIDUALS WITH SENSORINEURAL HEARING LOSS
Authors: Antony P Jawahar , Barman Animesh
Published: May 2021

Aim

To study auditory stream perception in sensorineural hearing loss using sinusoidally amplitude modulated signals (SAM).

Material and methods

There were 30 participants with normal hearing and 30 participants with SNHL who participated in the study. Two experiments were conducted. In Experiment I, an AB sequence of SAM stimuli was presented having a standard or reference modulation frequency in the A stimuli and a comparison or target modulation frequency in the B stimuli. In Experiment II, only a B stimuli sequence was presented. Both a low carrier frequency of 1 kHz and a high carrier frequency of 4 kHz were used in the experiment. A lower standard modulation frequency of 16 Hz and a higher standard modulation frequency of 256 Hz were considered. The comparison modulation frequencies ranged from 1 octave above to 4 octaves above the standard modulation frequencies. The objective listening task was to pick the irregularity in the rhythmic sequence when different levels of time delays were introduced, and this delay was used as a measure of stream perception.

Results

There was a significant difference between the normal hearing group and the SNHL group in their ability to detect irregularities when higher standard modulation frequencies were used, irrespective of the carrier frequencies. The SNHL group identified the irregularities better than the normal hearing group, indicating they had poorer stream perception.

Conclusions

Poorer stream perception with sinusoidal amplitude modulated tonal stimuli in the SNHL group can be attributed to poorer frequency resolution in the SNHL group.
AUDITORY EVALUATION IN A CASE OF HEARING LOSS IN AN INFANT WITH CONGENITAL ZIKA SYNDROME (CZS)
Authors: Sanfins Milaine , Brandão Lavinia , Skarzynski Piotr , Griz Silvana , Melo Adriana
Published: May 2021

Background

The Zika virus (ZIKV) was detected in Brazil in 2014. At the end of 2015 microcephaly was the first distinctive sign. By 2016, the first two cases of congenital Zika had been described, with a strong correlation between the virus and microcephaly.

Case report

A male infant was born at 32 weeks gestation, weight 1,750 grams, cranial perimeter 26 cm. He remained in intensive care for 5 days for neonatal seizures, required oxygen therapy, and had early sepsis. After 11 days in hospital a diagnosis of microcephaly was made. At 12 months, hydrocephalus was identified by computed tomography, with calcification foci in the subcortical region, basal nuclei, cerebellum, and brainstem. These characteristics are typical of congenital zika syndrome (CZS). Audiological tests were done: immittanciometry (tympanometry and acoustic reflex), transient evoked otoacoustic emission (TEOAE), distortion product otoacoustic emission (DPOAE), click ABR, and frequency following response (FFR). In patients with ZIKV, radiological findings show distinctive calcifications at the gray–white matter junction and degrees of delayed cortical development. The findings here of no acoustic reflex and a reduction in otoacoustic emissions in both ears is a preliminary indication of outer hair cell involvement that could later lead to communication deficits. ABR responses showed hearing pathway impairment in both ears up to the brainstem, and diminished FFR responses point to possible difficulties in speech perception. The present case study adds to our knowledge of the effect of CZS on the auditory system and strengthens the recommendation that ZIKV be included as an indicator for risk of hearing loss.
Histone extraction for mass spectrometry-based analysis of post-translational modifications in the fungal genus Aspergillus v1
Authors: Zhang Xin , Noberini Roberta , Bonaldi Tiziana , F. Seidl Michael , Collemare Jérȏme
Published: May 2021

Mass spectrometry is a powerful technique to uncover histone post-translational modifications in an unbiased and quantitative manner (Noberini,et al.,The FEBS Journal, 2021).Yet, such analyses require good quality protein samples, a challenge when working with fungi. Here, wepresenta protocol based on the work by Noberini and colleagues (Noberini, et al., Methods, 2020) toextract histoneproteinsfrom filamentous fungifor applications like mass spectrometry-based analyses of post-translational modifications. Several modifications were made: 1) additional cell disruption and homogenization stepsusingfreeze-drying and TissueLyser II; 2) increased Triton X-100 concentration in the lysis buffer; and 3) prolonged incubation time in lysis buffer. This protocol is reproducible, and the proteins extracted from threeAspergillusspecies (A. niger,A. fumigatus,andA. nidulans)wereof good quality for mass spectrometry.
Human intestinal cell dissociation suitable for multi-omics single-cell assays v1
Authors: Kosters Astrid , Yang Junkai , Dodd Ann , L White Mackenzie , Gibson Greg , Kugathasan Subra , Qiu Peng , Ghosn Eliver
Published: May 2021

We developed a protocol for the preparation of a single-cell suspension of human intestinal (ileal) biopsies, optimized for contemporary multi-omics single-cell assays including high-dimensional flow cytometry and single-cell RNA sequencing (scRNA-seq) assays. The protocol provides a rapid and efficient method of digesting human intestinal biopsies into a single-cell suspension resulting in a high viable cell yield and preservation of cell-surface markers. Notably, the protocol utilizes a Collagenase-I/Benzonase-based 37°C dissociation method, which collects already digested cells at several steps during the process to prevent over-digestion (fractioning technique). We obtained a total cell yield of ~5.8E6 +/- 1.9E6 cells, with a viability of 71% +/- 6% (n=9) (Figures 1A and 1B) from biopsy weights ranging 10-14 mg. By flow cytometry, all major immune cell lineages were detected, as well as a CD45-/EpCAM+ epithelial cell population (20%-60% of total viable singlets; Figure 1C). scRNA-seq analysis (10X Genomics; 5’/VDJ with feature barcoding) showed high-quality viable cells with low mitochondrial gene counts, indicating this protocol exerts minimal stress on the digested cells (Figure 1D).
Biodiversity as a complex clockwork/A biodiversidade como uma engrenagem complexa/La biodiversidad como un engranaje complejo
Authors: Brandão Reuber Albuquerque , Zanatta Maria Rosa Vargas , Souza Estevão do Nascimento Fernandes de
Published: May 2021

We discuss some ideas about the concepts of diversity and biodiversity, explaining the title of this text at the end, and how Heringeriana understands its role as a vehicle for the dissemination of new knowledge in these topics.
Discutimos algumas ideias sobre os conceitos de diversidade e biodiversidade, explicando ao final o tí­tulo desse texto e como a Heringeriana entende seu papel de veí­culo para a disseminação de novos conhecimentos nesses tópicos.
Discutimos algunas ideas sobre los conceptos de diversidad y biodiversidad, explicando al final el tí­tulo de este texto y cómo Heringeriana entiende su papel como vehí­culo para la difusión de nuevos conocimientos sobre estos temas.
PRAGMATIC POTENTIAL OF AUTHOR’S AND TRANSFORMED PHRASEOLOGIES IN THE WORK OF CARLOS RUIZ ZAFÓN «THE PRISONER OF HEAVEN»
Authors: Bovsunivska A. Yu. A. Yu.
Published: May 2021

The article is devoted to the study of pragmatic aspects of the use of phraseology in the textual space of Carlos Ruiz Safón’s novel «Prisoner of Heaven». One of the defining features of the individual style of this well-known modern Spanish writer is the metaphoricity and figuration of aristic expression, the saturation of the text with phraseological units that play a significant role in creating a pragmatic charge of the work of art.
Along with general linguistic phraseological units, which include commonly-used vocabulary, the author uses dialectal and authorial phraseological units, which is a feature of his individual style. All three designated groups of phraseological units mostly reflect the negative psychophysical and emotional state of the characters.
The author uses dialectal, individually-authorial and modified phraseological units, which is a feature of his individual style. It is determined that transformation is one of the most productive and most effective ways to update linguistic means in works of art. Author’s modification of FU leads to a change in the semantics and structure of expression, gives it a more expressive or emotional coloring. Transformed phraseology is limited to individual usage and is subject to the context of the work. Modified FUs in the Zafón’s artistic space acquire certain aesthetic and artistic qualities. Their modification is mainly to create the desired stylistic effect – to achieve emotional or expressive expression, which increases the reader’s interest, focuses on the content, issues of the work, as well as reveals the potential expressive potential of the Spanish language. In the transformed FUs, not just a new meaning is traced, but a combination of the well-known and the occasional. The unique combination of different types of phraseological units in the novel is considered a manifestation of individual style and makes a representation of the individually-authorial linguistic picture of the world more expressive.
Cataloging Of Unit Replacement Based On Long Run Repair Average Cost Rate (Acr) Based On Weibull Distribution Model
Authors: Et. al. Dr. K. Uma Maheswari,
Published: May 2021

Large amounts of money are lost each year in the real-estate industry because of poor schedule and cost control, In Industry the investigated failure and repair pattern, reliabilities of generators, compressors, turbines, using simple statistical tools and simulation techniques. The repair duration is divided into the 1)Major repair 2)Minor repair ,In major repair having(repair hour greater than a threshold valve)and Minor repair having(repair hour less than (or)equal to threshold valve).This approach is mainly for Weibull distribution method. In Weibull analysis is a common method for failure analysis and reliability engineering used in a wide range of applications. In this  paper, the applicability of Weibull analysis for evaluating and comparing the reliability of the schedule performance of multiple projects is presented, while the successive performance of multiple projects is presented ,while the successive repair times are increasing and are exposing  to Weibull distribution ,under these assumptions ,an optimal replacement policy ‘T’ in which we replace the system ,when the repair time reaches T. It can be determined that an optimal repair replacement policy T* such that long run average cost and the corresponding optimal replacement policy T* can be determined analytically.
Implementation of Boundary Alert system for Fishermen
Authors: Et. al. S. Niveda,
Published: May 2021

Fishing is one of the main occupations of the people who live near the coastal areas. In order to satisfy their daily requirements, fishermen need to get more fish. Once fishermen are not satisfied at a certain place, they move to further places to acquire more fish. Sometimes, without their knowledge they cross the border. Due to this, the navy of the other country arrests him and sentence them to prison. This happens because there is no awareness of boundary for the fishermen. In order to create an awareness for the fishermen, we proposed a system. Our proposed systemwill be an alternate source for avoiding such kind of accidents andalert the fishermen about the border area. In addition to that, in our project we implemented automatic turning of the boat in desired direction even when the alarm system fails to alert the fishermen by using RFtechnology. This is done byfixing the receiver in the boat and the transmitter has to be placed before 100 meters from the IMBL (International Marine time Boundary Line). This can be used to avoid crossing the boundary.
Comparative characteristics of the activities of a forensic specialist and a forensic investigator
Authors: Kostyuchenko O. G. , Volokhova O. V.
Published: May 2021

The article is devoted to the use of special knowledge in the investigation of crimes, the activities of a specialist criminalist, a forensic investigator, and a comparative description of their activities in the investigation of crimes. In the context of criminalization of society, which leads to the emergence of new types of crimes, more sophisticated ways to commit them and conceal them using sophisticated technical means, insufficient attention is obviously paid to the use of special knowledge, including forensic knowledge, carried out in both procedural and non-procedural forms, the tasks of using scientific and technical means and methods for the prevention, detection and investigation of crimes. Proper use of all forms of specialized knowledge will make more effective evidence that will serve to resolve the case in the future. The article is aimed at a forensic study of the features of the use of forensic knowledge in the detection and investigation of crimes. The article is based on the activities of the preliminary investigation bodies aimed at using special knowledge in the course of criminal proceedings, criminalistic patterns of typical situations involving knowledgeable persons in the investigation of crimes. The purpose of the research in the article is the criminalistic activity of a specialist criminalist and a forensic investigator in the detection and investigation of crimes.
The role of criminalistics in protecting assisted reproductive technologies from criminal risks
Authors: Kruchinina N. V.
Published: May 2021

The situation is analyzed in the article associated with an increase in the number of infertile couples, with the problems of determining reproductive human rights, with their protection and regulation. It is concluded that assisted reproductive technologies (in vitro fertilization, surrogacy, etc.) are effective methods of treating infertility. In the article the different points of view on human rights of reproduction are analyzed. It justifies the need to protect reproductive health. The number of cases of the use of assisted reproductive technologies in Russia is increasing every year. Legislation governing legal relations in this area needs further development. The role of criminalistics in this process is determined. A review of foreign legislation, regulating the field of assisted reproductive technologies is represented. An attempt is made to determine the list of crimes against human rights of reproduction and to consider them as an object of criminalistic research. The task of criminalistics is the development of effective technical, tactical and methodical recommendations for the detection and investigation crimes against human rights. This study is carried out with the financial support of the Russian Foundation for Fundamental Research in the framework of the research project № 18-29-14084.
USAGE OF CRIMINALISTIC TECHNICAL MEANS, METHODS AND DEVICES IN DETECTING AND INVESTIGATION OF VIOLATION OF THE ANTIMONOPOLY LEGISLATION, WHICH CONCERNED WITH CEASING OF COMPETITION
Authors: Milovanova M. M.
Published: May 2021

During detection and investigation of violations, the proper collection of appropriate evidences plays very important role in the detecting of criminal facts. That’s why the obtaining of relevant criminalistic information about the violation of Legislation, such as Cartel Collusions and Ceasing of Competition seems impossible without of using criminalistic technical means, methods and devices. This article reveals the opportunities of using criminalistic technologies which allows to detect and collect criminalistic information about Cartel Collusions. According to the complication of proofing the ceasing of competition facts, it’s very necessary to use certain effective criminalistic facilities in these cases.
On the issue of new forensic methods for establishing the limitation period for the execution of document details
Authors: Pichugin S. A.
Published: May 2021

The author critically examines the original forensic technique of “copying fragments of signature strokes (handwritten records) moistened with a system of solvents”. The provisions of this method contain the statement that the color of the coloring substance can be reliably determined when the studied document details were performed. The expert determines the categories of color shades visually (by eye). The comparative stage of the expert study is based on comparing the color of the dye used to make the document under study with the color scheme of the dye details of a similar method of execution, acting as comparison samples. As a result of the comparison of the colors of the dyes that were used to perform the studied details, and possibly the sample details, the expert has a personal opinion about the prescription of the studied document details. It is assumed that such a “technique” allows you to reliably establish the prescription of the details of the studied documents. The relevance of theoretical and practical analysis of the provisions of this “methodology” is associated with its active and unjustified use in solving expert problems in arbitration, civil and criminal proceedings.
Internet governance, borders and jurisdiction in the context of crime investigation
Authors: Zavialova D. V.
Published: May 2021

The article focuses on the technical features of Internet operation, causing legal and forensic consequences when investigating cybercrimes. Decentralized nature and the specificity of Internet governance resulting from its technical architecture lead to difficulties in identifying the jurisdiction during cybercrime investigations. In the article, the author also analyses IT crimes’ particular features, which make them difficult to investigate, as well as some contemporary ideas on the possibility of delineating borders on the Net, the consequences of such measures, and the ways to identify jurisdiction when looking into cybercrimes. Apart from that, the article examines the Internet governance system, managing organizations as well as their hierarchy, and accountability. The technical mechanism of Internet operation is analyzed. Some factors threatening the operation are reviewed. Following the research results, the author proposes some methods to enhance international cooperation regarding cybercrime investigations and resolving jurisdictional issues. The need to increase informational and technical knowledge at all levels and to support interdepartmental cooperation is noted.
The method of combined use of enzyme therapy and carboxytherapy in the correction of complications after injection of polylactic acid in cosmetology
Authors: Aksenenko Irina P.
Published: May 2021

Objective. To study the effectiveness of the combined method, including the course of colladizine diadynamophoresis 1000 KE (collagenase units) in combination with injection carboxytherapy in the correction of complications that occurred when superficial administration of polylactic acid into the skin.
Material and methods. Under observation were 38 women of 32 to 39 years old with a complication after the introduction of a filler based on polylactic acid with localization in the abdomen. Three groups were identified that received a course of carboxytherapy, a course of collagenase diadynamophoresis, and their combination.
Results. The use of the combined course of Collizin diadynamophoresis with injection carboxytherapy provides a pronounced analgesic effect, improves microcirculation, stabilizes the psycho-emotional state and gives a pronounced effect of reducing the thickness of the dermis and increasing the density coefficient of the dermis, which is probably associated with a decrease in intradermal edema, which occurs as a reaction to superficially introduced filler. In addition, this combined technique significantly accelerates the biodegradation of the surface-injected filler and affects pathological fibrosis around polylactic acid.
Conclusions. The combined use of collizine diadynamophoresis and injection carboxytherapy locally on the lesion sites with complications such as surface administration of a filler based on polylactic acid is a highly effective treatment method, which is confirmed by the relief of clinical symptoms and an increase in the quality of life of patients.
Justification of the complex application of physical therapy methods in the treatment of visual analyzer diseases
Authors: Dragon Alina K. , Korchazhkina Natalia B. , Katsnelson Victoria V.
Published: May 2021

The article presents the data of clinical and experimental scientific studies of Russian scientists on the use of physiotherapy methods in clinical practice in various metabolic-dystrophic and degenerative diseases of the eye and the auxiliary apparatus and modern approaches to the formation of programs for the complex and combined use of natural and preformed factors for the treatment and prevention of diseases of the visual analyzer.
Magnetotherapy in clinical practice
Authors: Maksimov Aleksandr V. , Kiryanova Vera V.
Published: May 2021

This article discusses mechanisms of physiological and therapeutic effects of magnetic fields, characteristics of magnetic fields effect on main functional and regulatory systems of the human body, as well as therapeutic techniques, indications and contraindications for therapeutic use, fundamentals of physics, and biophysics of magnetism. It also presents modern magnetic therapy devices, equipment, and methods of performing medical procedures.
Evaluation of shock index and modified shock index in estimation of MACE parameters in patients with ST elevated myocardial infarction
Authors: AKYOL Pınar Yeşim , KARAKAYA Zeynep , TOPAL Fatih , URNAL Rıfat , ACAR Murat , PAYZA Umut , BİLGİN Serkan
Published: May 2021

Purpose: Shock index and modified shock index were used to evaluate the hemodynamic status of patients with trauma, pulmonary embolism and aortic dissection. In this study, we aimed to evaluate the effectiveness of shock index and modified shock index as an indicator of major adverse cardiac event parameters in patients with ST elevated myocardial infarction.

Materials and Methods: A total of 194 patients with ST elevated myocardial infarction were included in the study. Shock index and modified shock index were evaluated regarded to predicting major adverse cardiac event and major adverse cardiac event parameters separately.

Results: A total of 194 patients were included in the study. The 7-day mortality was 2.4% in the shock index