bluuebunny/crossref_metadata_2025 huggingface dataset:
Prognostic signature of lung adenocarcinoma based on stem cell-related genes
Authors: Huang Zhanghao , Shi Muqi , Zhou Hao , Wang Jinjie , Zhang Hai-Jian , Shi Jia -Hai
Published: January 2021

Lung adenocarcinoma (LUAD) is characterized by high infiltration and rapid growth. The function of the stem cell population is to control and maintain cell regeneration. Therefore, it is necessary to study the prognostic value of stem cell-related genes in LUAD. Signature genes were screened out from 166 stem cell-related genes according to the least absolute shrinkage operator (LASSO) and subsequently multivariate Cox regression analysis, and then established risk model. Immune infiltration and nomogram model were used to evaluate the clinical efficacy of signature. A signature consisting of 10 genes was used to dichotomize the LUAD patients into two groups (cutoff, 1.314), and then validated in GSE20319 and GSE42127. There was a significant correlation between signature and clinical characteristics. Patients with high-risk had a shorter overall survival. Furthermore, significant differences were found in multiple immune cells between the high-risk group and low-risk group. A high correlation was also reflected between signature and immune infiltration. What’s more, the signature could effectively predict the efficacy of chemotherapy in patients with LUAD, and a nomogram based on signature might accurately predict the prognosis of patients with LUAD. The signature-based of stem cell-related genes might be contributed to predicting prognosis of patients with LUAD.
The effect of taxonomic classification by full-length 16S rRNA sequencing with a synthetic long-read technology
Authors: Jeong Jinuk , Yun Kyeongeui , Mun Seyoung , Chung Won-Hyong , Choi Song-Yi , Nam Young-do , Lim Mi Young , Hong Chang Pyo , Park ChanHyeok , Ahn Yong Ju , Han Kyudong
Published: January 2021

Characterizing the microbial communities inhabiting specimens is one of the primary objectives of microbiome studies. A short-read sequencing platform for reading partial regions of the 16S rRNA gene is most commonly used by reducing the cost burden of next-generation sequencing (NGS), but misclassification at the species level due to its length being too short to consider sequence similarity remains a challenge. Loop Genomics recently proposed a new 16S full-length-based synthetic long-read sequencing technology (sFL16S). We compared a 16S full-length-based synthetic long-read (sFL16S) and V3-V4 short-read (V3V4) methods using 24 human GUT microbiota samples. Our comparison analyses of sFL16S and V3V4 sequencing data showed that they were highly similar at all classification resolutions except the species level. At the species level, we confirmed that sFL16S showed better resolutions than V3V4 in analyses of alpha-diversity, relative abundance frequency and identification accuracy. Furthermore, we demonstrated that sFL16S could overcome the microbial misidentification caused by different sequence similarity in each 16S variable region through comparison the identification accuracy of Bifidobacterium, Bacteroides, and Alistipes strains classified from both methods. Therefore, this study suggests that the new sFL16S method is a suitable tool to overcome the weakness of the V3V4 method.
Deep learning-Based 3D inpainting of brain MR images
Authors: Kang Seung Kwan , Shin Seong A. , Seo Seongho , Byun Min Soo , Lee Dong Young , Kim Yu Kyeong , Lee Dong Soo , Lee Jae Sung
Published: January 2021

The detailed anatomical information of the brain provided by 3D magnetic resonance imaging (MRI) enables various neuroscience research. However, due to the long scan time for 3D MR images, 2D images are mainly obtained in clinical environments. The purpose of this study is to generate 3D images from a sparsely sampled 2D images using an inpainting deep neural network that has a U-net-like structure and DenseNet sub-blocks. To train the network, not only fidelity loss but also perceptual loss based on the VGG network were considered. Various methods were used to assess the overall similarity between the inpainted and original 3D data. In addition, morphological analyzes were performed to investigate whether the inpainted data produced local features similar to the original 3D data. The diagnostic ability using the inpainted data was also evaluated by investigating the pattern of morphological changes in disease groups. Brain anatomy details were efficiently recovered by the proposed neural network. In voxel-based analysis to assess gray matter volume and cortical thickness, differences between the inpainted data and the original 3D data were observed only in small clusters. The proposed method will be useful for utilizing advanced neuroimaging techniques with 2D MRI data.
Phytoplankton consortia as a blueprint for mutually beneficial eukaryote-bacteria ecosystems based on the biocoenosis of Botryococcus consortia
Authors: Blifernez-Klassen Olga , Klassen Viktor , Wibberg Daniel , Cebeci Enis , Henke Christian , Rückert Christian , Chaudhari Swapnil , Rupp Oliver , Blom Jochen , Winkler Anika , Al-Dilaimi Arwa , Goesmann Alexander , Sczyrba Alexander , Kalinowski Jörn , Bräutigam Andrea , Kruse Olaf
Published: January 2021

Bacteria occupy all major ecosystems and maintain an intensive relationship to the eukaryotes, developing together into complex biomes (i.e., phycosphere and rhizosphere). Interactions between eukaryotes and bacteria range from cooperative to competitive, with the associated microorganisms affecting their host`s development, growth and health. Since the advent of non-culture dependent analytical techniques such as metagenome sequencing, consortia have been described at the phylogenetic level but rarely functionally. Multifaceted analysis of the microbial consortium of the ancient phytoplankton Botryococcus as an attractive model food web revealed that its all abundant bacterial members belong to a niche of biotin auxotrophs, essentially depending on the microalga. In addition, hydrocarbonoclastic bacteria without vitamin auxotrophies seem adversely to affect the algal cell morphology. Synthetic rearrangement of a minimal community consisting of an alga, a mutualistic and a parasitic bacteria underpins the model of a eukaryote that maintains its own mutualistic microbial community to control its surrounding biosphere. This model of coexistence, potentially useful for defense against invaders by a eukaryotic host could represent ecologically relevant interactions that cross species boundaries. Metabolic and system reconstruction is an opportunity to unravel the relationships within the consortia and provide a blueprint for the construction of mutually beneficial synthetic ecosystems.
Ultra-broadband axicon transducer for optoacoustic endoscopy
Authors: Ali Zakiullah , Zakian Christian , Ntziachristos Vasilis
Published: January 2021

Image performance in optoacoustic endoscopy depends markedly on the design of the transducer employed. Ideally, high-resolution performance is required over an expanded depth of focus. Current optoacoustic focused transducers achieve lateral resolutions in the range of tens of microns in the mesoscopic regime, but their depth of focus is limited to hundreds of microns by the nature of their spherical geometry. We designed an ultra-broadband axicon detector with a 2 mm central aperture and investigated whether the imaging characteristics exceeded those of a spherical detector of similar size. We show a previously undocumented ability to achieve a broadband elongated pencil-beam optoacoustic sensitivity with an axicon detection geometry, providing approximately 40 μm-lateral resolution maintained over a depth of focus of 950 μm—3.8 times that of the reference spherical detector. This performance could potentially lead to optoacoustic endoscopes that can visualize optical absorption deeper and with higher resolution than any other optical endoscope today.
Integrated analysis of lncRNA and mRNA transcriptomes reveals the potential regulatory role of lncRNA in kiwifruit ripening and softening
Authors: Chen Yiting , Cheng Chunzhen , Feng Xin , Lai Ruilian , Gao Minxia , Chen Wenguang , Wu Rujian
Published: January 2021

Kiwifruit has gained increasing attention worldwide for its unique flavor and high nutritional value. Rapid softening after harvest greatly shortens its shelf-life and reduces the commercial value. Therefore, it is imperative and urgent to identify and clarify its softening mechanism. This study aimed to analyze and compare the long noncoding RNA (lncRNA) and mRNA expression patterns in ABA-treated (ABA) and room temperature (RT)-stored fruits with those in freshly harvested fruits (CK) as control. A total of 697 differentially expressed genes (DEGs) and 81 differentially expressed lncRNAs (DELs) were identified while comparing ABA with CK, and 458 DEGs and 143 DELs were detected while comparing RT with CK. The Kyoto Encyclopedia of Genes and Genomes analysis of the identified DEGs and the target genes of DELs revealed that genes involved in starch and sucrose metabolism, brassinosteroid biosynthesis, plant hormone signal transduction, and flavonoid biosynthesis accounted for a large part. The co-localization networks, including 38 DEGs and 31 DELs in ABA vs. CK, and 25 DEGs and 25 DELs in RT vs. CK, were also performed. Genes related to fruit ripening, such as genes encoding β-galactosidase, mannan endo-1,4-β-mannosidase, pectinesterase/pectinesterase inhibitor, and NAC transcription factor, were present in the co-localization network, suggesting that lncRNAs were involved in regulating kiwifruit ripening. Notably, several ethylene biosynthesis- and signaling-related genes, including one 1-aminocyclopropane-1-carboxylic acid oxidase gene and three ethylene response factor genes, were found in the co-localization network of ABA vs. CK, suggesting that the promoting effect of ABA on ethylene biosynthesis and fruit softening might be embodied by increasing the expression of these lncRNAs. These results may help understand the regulatory mechanism of lncRNAs in ripening and ABA-induced fruit softening of kiwifruit.
Graphene oxide loaded with tumor-targeted peptide and anti-cancer drugs for cancer target therapy
Authors: Li Ran , Wang Yimei , Du Jie , Wang Xiangyu , Duan Ailin , Gao Ruifang , Liu Junyu , Li Bing
Published: January 2021

In the present work, we constructed nanoscale graphene oxide (NGO) as a drug nanocarrier to improve the process of tumor-targeted drug releases, promote cellular uptake and accumulation of chemotherapy drugs in tumor tissues, and reduce the toxic effects of chemotherapy drugs on normal cells. Hence, great stability was obtained in the biological solution. Moreover, we designed an effective nanoparticle system for the doxorubicin (DOX) delivery targeting the oral squamous cell carcinoma (OSCC) by mediating the HN-1 (TSPLNIHNGQKL) through hydrogen and π–π bonds. DOX@NGO-PEG-HN-1 showed significantly higher cellular uptakes and cytotoxicity in OSCC cells (CAL-27 and SCC-25), compared to free DOX. Moreover, HN-1 showed considerable tumor-targeting and competition inhibition phenomenon. As we expected, the nanocarrier showed pH-responsive drug release. In total, our study represented a good technique to construct OSCC-targeted delivery of nanoparticles and improve the anticancer medicines’ efficiency.
Bisphenol A, S or F mother’s dermal impregnation impairs offspring immune responses in a dose and sex-specific manner in mice
Authors: Malaisé Yann , Lencina Corinne , Cartier Christel , Olier Maïwenn , Ménard Sandrine , Guzylack-Piriou Laurence
Published: January 2021

Bisphenol (BP)A is an endocrine disruptor (ED) widely used in thermal papers. Regulatory restrictions have been established to prevent risks for human health, leading to BPA substitution by structural analogues, like BPS and BPF. We previously demonstrated that oral perinatal exposure to BPA had long-term consequences on immune responses later in life. It appears now essential to enhance our understanding on immune impact of different routes of BP exposure. In this study, we aimed at comparing the impact of mother dermal exposure to BPs on offspring immune system at adulthood. Gravid mice were dermally exposed to BPA, BPS or BPF at 5 or 50 μg/kg of body weight (BW)/day (d) from gestation day 15 to weaning of pups at post-natal day (PND)21. In offspring, BPs dermal impregnation of mothers led to adverse effects on immune response at intestinal and systemic levels that was dependent on the BP, the dose and offspring sex. These findings provide, for the first time, results on long-term consequences of dermal perinatal BPs exposure on immune responses in offspring. This work warns that it is mandatory to consider immune markers, dose exposure as well as sex in risk assessment associated with new BPA’s alternatives.
An optimised method for intact nuclei isolation from diatoms
Authors: Annunziata Rossella , Balestra Cecilia , Marotta Pina , Ruggiero Antonella , Manfellotto Francesco , Benvenuto Giovanna , Biffali Elio , Ferrante Maria Immacolata
Published: January 2021

Due to their abundance in the oceans, their extraordinary biodiversity and the increasing use for biotech applications, the study of diatom biology is receiving more and more attention in the recent years. One of the limitations in developing molecular tools for diatoms lies in the peculiar nature of their cell wall, that is made of silica and organic molecules and that hinders the application of standard methods for cell lysis required, for example, to extract organelles. In this study we present a protocol for intact nuclei isolation from diatoms that was successfully applied to three different species: two pennates, Pseudo-nitzschia multistriata and Phaeodactylum tricornutum, and one centric diatom species, Chaetoceros diadema. Intact nuclei were extracted by treatment with acidified NH4F solution combined to low intensity sonication pulses and separated from cell debris via FAC-sorting upon incubation with SYBR Green. Microscopy observations confirmed the integrity of isolated nuclei and high sensitivity DNA electrophoresis showed that genomic DNA extracted from isolated nuclei has low degree of fragmentation. This protocol has proved to be a flexible and versatile method to obtain intact nuclei preparations from different diatom species and it has the potential to speed up applications such as epigenetic explorations as well as single cell (“single nuclei”) genomics, transcriptomics and proteomics in different diatom species.
Radiolysis generates a complex organosynthetic chemical network
Authors: Adam Zachary R. , Fahrenbach Albert C. , Jacobson Sofia M. , Kacar Betul , Zubarev Dmitry Yu.
Published: January 2021

The architectural features of cellular life and its ecologies at larger scales are built upon foundational networks of reactions between molecules that avoid a collapse to equilibrium. The search for life’s origins is, in some respects, a search for biotic network attributes in abiotic chemical systems. Radiation chemistry has long been employed to model prebiotic reaction networks, and here we report network-level analyses carried out on a compiled database of radiolysis reactions, acquired by the scientific community over decades of research. The resulting network shows robust connections between abundant geochemical reservoirs and the production of carboxylic acids, amino acids, and ribonucleotide precursors—the chemistry of which is predominantly dependent on radicals. Moreover, the network exhibits the following measurable attributes associated with biological systems: (1) the species connectivity histogram exhibits a heterogeneous (heavy-tailed) distribution, (2) overlapping families of closed-loop cycles, and (3) a hierarchical arrangement of chemical species with a bottom-heavy energy-size spectrum. The latter attribute is implicated with stability and entropy production in complex systems, notably in ecology where it is known as a trophic pyramid. Radiolysis is implicated as a driver of abiotic chemical organization and could provide insights about the complex and perhaps radical-dependent mechanisms associated with life’s origins.
The interaction of Ag2O nanoparticles with Escherichia coli: inhibition–sterilization process
Authors: Li Danqing , Chen Shuai , Zhang Ke , Gao Nan , Zhang Miao , Albasher Gadah , Shi Jiangfan , Wang Chuanyi
Published: January 2021

Silver-based antibacterial agents have obtained wide attention due to the fact that bacteria in the environment is ubiquitous, which has become one of the most difficult problems for human health. However, the antibacterial mechanism and process are still inconclusive. Here, Ag2O nanoparticles (NPs) with uniform spherical morphology and small size (around 30 nm) were prepared. The as-prepared Ag2O NPs induced high antibacterial activity (100% inhibition ratio) against E. coli. A two-step antibacterial process was proposed and confirmed, which divided into inhibition and sterilization steps. The optical density measurement, malondialdehyde concentration detection, morphologic imaging with electronic microscopy and Fourier transform infrared spectroscopic analysis unveiled the interaction of Ag2O NPs with E. coli, which verified the inhibition–sterilization process we proposed.
Perinatal SSRI exposure affects brain functional activity associated with whisker stimulation in adolescent and adult rats
Authors: Van der Knaap Noortje , Wiedermann Dirk , Schubert Dirk , Hoehn Mathias , Homberg Judith R.
Published: January 2021

Selective serotonin reuptake inhibitors (SSRI), such as fluoxetine, are used as first-line antidepressant medication during pregnancy. Since SSRIs cross the placenta the unborn child is exposed to the maternal SSRI medication, resulting in, amongst others, increased risk for autism in offspring. This likely results from developmental changes in brain function. Studies employing rats lacking the serotonin transporter have shown that elevations in serotonin levels particularly affect the development of the whisker related part of the primary somatosensory (barrel) cortex. Therefore, we hypothesized that serotonin level disturbances during development alter brain activity related to whisker stimulation. We treated female dams with fluoxetine or vehicle from gestational day 11 onwards for 21 days. We investigated offspring’s brain activity during whisker stimulation using functional magnetic resonance imaging (fMRI) at adolescence and adulthood. Our results indicate that adolescent offspring displayed increased activity in hippocampal subareas and the mammillary body in the thalamus. Adult offspring exhibited increased functional activation of areas associated with (higher) sensory processing and memory such as the hippocampus, perirhinal and entorhinal cortex, retrospinal granular cortex, piriform cortex and secondary visual cortex. Our data imply that perinatal SSRI exposure leads to complex alterations in brain networks involved in sensory perception and processing.
Complex networks of marine heatwaves reveal abrupt transitions in the global ocean
Authors: Benedetti-Cecchi Lisandro
Published: January 2021

Understanding how marine heatwaves (MHWs) unfold in space and time under anthropogenic climate change is key to anticipate future impacts on ecosystems and society. Yet, our knowledge of the spatiotemporal dynamics of MHWs is very limited. Here, I combine network theory with topological data analysis and event synchronization to high-resolution satellite data and to a set of Earth System Model simulations to reveal the dynamical organization of complex MHW networks. The analysis reveals that MHWs have already crossed a tipping point separating highly synchronized preindustrial MHWs from the more extreme, but less coherent warming events we experience today. This loose spatiotemporal organization persists under a reduced RCP 2.6 emission scenario, whereas a second abrupt transition towards a permanent state of highly synchronized MHWs is foreseen by 2075 under a business-as-usual RCP 8.5 scenario. These results highlight the risks of abrupt ocean transitions, which may dramatically affect marine life and humanity by eroding valuable time for adaptation to climate change.
Efficacy of therapies and interventions for repeated embryo implantation failure: a systematic review and meta-analysis
Authors: Busnelli Andrea , Somigliana Edgardo , Cirillo Federico , Baggiani Annamaria , Levi-Setti Paolo Emanuele
Published: January 2021

The aim of the present systematic review and meta-analysis was to assess the effect of the different therapeutic options for repeated embryo implantation failure (RIF) on a subsequent IVF cycle outcome. Twenty-two RCTs and nineteen observational studies were included. Pooling of results showed a beneficial effect of intrauterine PBMC infusion on both CPR (RR 2.18; 95% CI 1.58–3.00; p < 0.00001; OR 2.03; 95% CI 1.22–3.36; p = 0.006) and LBR (RR 2.41; 95% CI 1.40–4.16; p = 0.002; OR 3.73; 95% CI 1.13–12.29; p = 0.03), of subcutaneous G-CSF administration on CPR (RR 2.29; 95% CI 1.58–3.31; p < 0.0001) and of intrauterine PRP infusion on CPR (RR 2.45; 95% CI 1.55–3.86; p = 0.0001). Observational studies also demonstrated a positive effect of IVIG and intrauterine hCG infusion on both CPR and LBR and of atosiban on CPR. Studies investigating intrauterine G-CSF infusion, LMWH, intravenous intralipid, hysteroscopy, blastocyst-stage ET, ZIFT, PGT-A and AH failed to observe an impact on IVF outcome. The quality of the evidence that emerged from RCTs focused on intrauterine PBMC infusion and subcutaneous G-CSF administration was moderate. For all other therapies/interventions it varied from low to very low. In conclusion, intrauterine PBMC infusion and subcutaneous G-CSF administration are the most promising therapeutic options for RIF. However, further well conducted RCTs are necessary before their introduction into clinical practice.
Simulated atmospheric nitrogen deposition inhibited the leaf litter decomposition of Cinnamomum migao H. W. Li in Southwest China
Authors: Huang Xiao-Long , Chen Jing-Zhong , Wang Deng , Deng Ming-Ming , Wu Meng-Yao , Tong Bing-Li , Liu Ji-Ming
Published: January 2021

Atmospheric nitrogen (N) deposition could affect various ecological processes in forest ecosystems, including plant litter decomposition and nutrient cycling. However, the mechanism of underlying litter decomposition and nutrient cycling of Cinnamomum migao under N deposition remains unclear. Therefore, we conducted a simulated N deposition experiment including four onsite treatments to assess the effects of N input on C. migao leaf litter decomposition, nutrient release, and soil enzyme activity. The results showed that simulated N deposition significantly increased the amount of total residual mass and lignin and cellulose, decreased the decomposition rate, and suppressed net nutrient release. N input increased C, N, and P ratios as decomposition progressed, and the proportion of mass remaining was positively correlated with the proportions of lignin and cellulose remaining at the later stage of decomposition. The differences in soil enzyme activity were primarily due to enzyme type and sampling time. We conclude that simulated N deposition significantly suppressed the leaf litter decomposition of C. migao by mainly altering the chemical properties and suppressing the decomposition of the organic matter in leaf litter. Lignin might have played an important role in the loss of leaf litter biomass at the later stage of decomposition.
Understanding adherence to reactive treatment of asymptomatic malaria infections in The Gambia
Authors: Jaiteh Fatou , Okebe Joseph , Masunaga Yoriko , D’Alessandro Umberto , Achan Jane , Gryseels Charlotte , de Vries Daniel , Ribera Joan Muela , Grietens Koen Peeters
Published: January 2021

The impact of different types of reactive case detection and/or treatment strategies for malaria elimination depends on high coverage and participants’ adherence. However, strategies to optimise adherence are limited, particularly for people with asymptomatic or no infections. As part of a cluster-randomized trial to evaluate the effect of reactive treatment in The Gambia, all residents in the compound of a diagnosed clinical malaria patient received dihydro-artemisinin–piperaquine (DP). Using a mixed method approach, we assessed which factors contribute to adherence among the contacts of malaria cases that showed no symptoms. Adherence was defined as the proportion of compound members that (1) returned all medicine bags empty and (2) self-reported (3-day) treatment completion. Among the 273 individuals from 14 compounds who received DP, 227 (83.1%) were available for and willing to participate in the survey; 85.3% (233/273) returned empty medicine bags and 91.6% (208/227) self-reported treatment completion. Although clinical malaria was not considered a major health problem, reported adherence was high. The drivers of adherence were the strong sense of responsibility towards protecting the individual, compound and the village. Adherence can be optimised through a transdisciplinary implementation research process of engaging communities to bridge the gap between research goals and social realities.
Genome analysis of the monoclonal marbled crayfish reveals genetic separation over a short evolutionary timescale
Authors: Maiakovska Olena , Andriantsoa Ranja , Tönges Sina , Legrand Carine , Gutekunst Julian , Hanna Katharina , Pârvulescu Lucian , Novitsky Roman , Weiperth András , Sciberras Arnold , Deidun Alan , Ercoli Fabio , Kouba Antonin , Lyko Frank
Published: January 2021

The marbled crayfish (Procambarus virginalis) represents a very recently evolved parthenogenetic freshwater crayfish species that has invaded diverse habitats in Europe and in Madagascar. However, population genetic analyses have been hindered by the homogeneous genetic structure of the population and the lack of suitable tools for data analysis. We have used whole-genome sequencing to characterize reference specimens from various known wild populations. In parallel, we established a whole-genome sequencing data analysis pipeline for the population genetic analysis of nearly monoclonal genomes. Our results provide evidence for systematic genetic differences between geographically separated populations and illustrate the emerging differentiation of the marbled crayfish genome. We also used mark-recapture population size estimation in combination with genetic data to model the growth pattern of marbled crayfish populations. Our findings uncover evolutionary dynamics in the marbled crayfish genome over a very short evolutionary timescale and identify the rapid growth of marbled crayfish populations as an important factor for ecological monitoring.
A chicken DNA methylation clock for the prediction of broiler health
Authors: Raddatz Günter , Arsenault Ryan J. , Aylward Bridget , Whelan Rose , Böhl Florian , Lyko Frank
Published: January 2021

The domestic chicken (Gallus gallus domesticus) is the globally most important source of commercially produced meat. While genetic approaches have played an important role in the development of chicken stocks, little is known about chicken epigenetics. We have systematically analyzed the chicken DNA methylation machinery and DNA methylation landscape. While overall DNA methylation distribution was similar to mammals, sperm DNA appeared hypomethylated, which correlates with the absence of the DNMT3L cofactor in the chicken genome. Additional analysis revealed the presence of low-methylated regions, which are conserved gene regulatory elements that show tissue-specific methylation patterns. We also used whole-genome bisulfite sequencing to generate 56 single-base resolution methylomes from various tissues and developmental time points to establish an LMR-based DNA methylation clock for broiler chicken age prediction. This clock was used to demonstrate epigenetic age acceleration in animals with experimentally induced inflammation. Our study provides detailed insights into the chicken methylome and suggests a novel application of the DNA methylation clock as a marker for livestock health.
Simultaneous determination of six glycosidic aroma precursors in pomelo by ultra-high performance liquid chromatography-tandem mass spectrometry
Authors: Su Desen , Zheng Yunyun , Chen Ziqiang , Chi Yuwu
Published: January 2021

A rapid, accurate and efficient UPLC-MS/MS method was established for the simultaneous determination of six glycosidic aroma precursors in pomelo.
Self-powered liquid chemical sensors based on solid–liquid contact electrification
Authors: Ying Zhihua , Long Yin , Yang Fan , Dong Yutao , Li Jun , Zhang Ziyi , Wang Xudong
Published: January 2021

The liquid-solid contact electrification was used as a sensing mechanism to analyze liquid chemicals, which may eventually become a new molecule analytic principle.
Correction: Aptamer-based strategies for recognizing adenine, adenosine, ATP and related compounds
Authors: Li Yuqing , Liu Juewen
Published: January 2021

Correction for ‘Aptamer-based strategies for recognizing adenine, adenosine, ATP and related compounds’ by Yuqing Li et al., Analyst, 2020, 145, 6753–6768, DOI: 10.1039/D0AN00886A.
Deep eutectic solvent based ultrasound assisted emulsification microextraction for preconcentration and voltammetric determination of aflatoxin B1 in cereal samples
Authors: Zohdijamil Zeynab , Reza Ahmadi Afshar Seyed Ahmad , Khazalpour Sadegh , Hashemi Mahdi
Published: January 2021

A new and simple deep eutectic solvent based ultrasound-assisted emulsification microextraction (DES-UAEME) procedure has been developed for preconcentration and voltammetric determination of aflatoxin B1 (AFB1) in cereal products.
amTCO, a newtrans-cyclooctene derivative to study drug-target interactions in cells
Authors: Echalier Cécile , Rutkowska Anna , Kojic Ana , Thomson Douglas W. , Edwards Lee J. , McKay Blandine S. J. , Mülbaier Marcel , Schultz Carsten , Bergamini Giovanna
Published: January 2021

We provide a new tagging entity for click chemistry to perform improved drug binding assays in intact cells.
Visible-light promoted α-alkylation of glycine derivatives with alkyl boronic acids
Authors: Wang Jiayuan , Su Yingpeng , Quan Zhengjun , Li Jun , Yang Jie , Yuan Yong , Huo Congde
Published: January 2021

A visible-light-mediated aerobic α-alkylation reaction of glycine derivatives with alkyl boronic acids has been established in the presence of a Ru/Cu catalyst system, giving the desired radical coupling products efficiently.
3-Thiolated pyrroles/pyrrolines: controllable synthesis and usage for the construction of thiolated fluorophores
Authors: Tian Jun , Yuan Kang-Ning , Liu Wen , Chang Hong-Hong , Li Xing , Gao Wen-Chao
Published: January 2021

Controllable synthesis of 3-thiolated pyrroles or pyrrolines was achieved by tuning the reaction solvents, allowing the efficient construction of bisthiolated BODIPY.
Character angle effects on dissociated dislocation core energy in aluminum
Authors: Zhou X. W. , Foster M. E.
Published: January 2021

Dislocation core energy is an important property in materials mechanics but can only be obtained from atomistic simulations.
Heterogeneity in hydrophobic deep eutectic solvents: SAXS prepeak and local environments
Authors: Malik Akshay , Kashyap Hemant K.
Published: January 2021

The observation of the prepeak in the simulated total X-ray scattering structure function (S(q)) reveals the presence of intermediate-range structural heterogeneity in hydrophobic deep eutectic solvents.
Structural property-induced different phonon-twin-boundary scattering in diamond
Authors: Dong Huicong , Yu Shuaichao , Feng Zhihao , Wen Bin
Published: January 2021

Twin boundary thermal resistances vary significantly with their structures, and they are strongly correlated with twin boundary energy and bond difference parameter.
Selective saturation of step-edges as a tool to control the growth of molecular fibres
Authors: Dreher Maximilian , Witte Gregor
Published: January 2021

The formation of molecular fibres is often hampered by defects such as step edges, which act as nucleation sites. Here, we present a concept of how exposure of the support to oxygen or even air disables the formation of such defect-driven fibres.
Reactive intermediates for interactome mapping
Authors: Seath Ciaran P. , Trowbridge Aaron D. , Muir Tom W. , MacMillan David W. C.
Published: January 2021

This tutorial review describes enabling methods for determining biomolecular interactions in live cells through the use of in situ generated reactive intermediates.
CO2hydrogenation to methanol and hydrocarbons over bifunctional Zn-doped ZrO2/zeolite catalysts
Authors: Ticali Pierfrancesco , Salusso Davide , Ahmad Rafia , Ahoba-Sam Christian , Ramirez Adrian , Shterk Genrikh , Lomachenko Kirill A. , Borfecchia Elisa , Morandi Sara , Cavallo Luigi , Gascon Jorge , Bordiga Silvia , Olsbye Unni
Published: January 2021

The tandem process of carbon dioxide hydrogenation to methanol and its conversion to hydrocarbons over mixed metal/metal oxide-zeotype catalysts is a promising path to CO2valorization.
Effects of thermal processing on the allergenicity, structure, and critical epitope amino acids of crab tropomyosin
Authors: Liu Meng , Han Tian-Jiao , Huan Fei , Li Meng-Si , Xia Fei , Yang Yang , Wu Yun-Hui , Chen Gui-Xia , Cao Min-Jie , Liu Guang-Ming
Published: January 2021

Food processing can change the structure and immunoreactivity of purified allergens, but the effect of food processing on the immunoreactivity of the processed and purified allergen is still poorly understood.
Tannic acid–thioctic acid hydrogel: a novel injectable supramolecular adhesive gel for wound healing
Authors: Chen Chen , Yang Xiao , Li Shu-jing , Zhang Chao , Ma Yu-ning , Ma Yu-xia , Gao Peng , Gao Shu-zhong , Huang Xiao-jun
Published: January 2021

Thiyl radical–polyphenol Michael addition was utilized to prepare tannic acid–thioctic acid (TATA) hydrogel under mild condition. It was also found that this hydrogel could be used as wound adhesive with good biocompatibility.
Efficient electrochemical synthesis of a manganese-based metal–organic framework for H2 and CO2 uptake
Authors: Asghar Aisha , Iqbal Naseem , Noor Tayyaba , Kariuki Benson M. , Kidwell Luke , Easun Timothy L.
Published: January 2021

Electrochemical synthesis, from manganese strips and dissolved linker, of a new amine-containing manganese-based metal–organic framework with enhanced CO2 uptake.
Total reflection X-ray fluorescence analysis of aerosol particles with direct dissolution of the collection filter on a substrate
Authors: Matsuyama Tsugufumi , Yamaguchi Hiroki , Tsuji Kouichi
Published: January 2021

In this study, a novel method based on total reflection X-ray analysis was developed for determining the trace elements in aerosol particles.
Multi-element analysis of minerals using laser ablation inductively coupled plasma time of flight mass spectrometry and geochemical data visualization using t-distributed stochastic neighbor embedding: case study on emeralds
Authors: Wang Hao A. O. , Krzemnicki Michael S.
Published: January 2021

A concept of “first measure, then determine” which elements are of interest is introduced for multi-element analysis using LA-ICP-TOF-MS. Geochemical data visualization using t-SNE is advantageous over PCA for determining the provenances of emerald.
Healthy and diseasedin vitromodels of vascular systems
Authors: Hosseini Vahid , Mallone Anna , Nasrollahi Fatemeh , Ostrovidov Serge , Nasiri Rohollah , Mahmoodi Mahboobeh , Haghniaz Reihaneh , Baidya Avijit , Salek M. Mehdi , Darabi Mohammad Ali , Orive Gorka , Shamloo Amir , Dokmeci Mehmet R. , Ahadian Samad , Khademhosseini Ali
Published: January 2021

A critical review of healthy and diseasedin vitromodels of the vascular system and in particular for atherosclerosis, aneurysm, and thrombosis.
Optimization of aptamer selection on an automated microfluidic system with cancer tissues
Authors: Lin Cheng-Sheng , Tsai Yi-Cheng , Hsu Keng-Fu , Lee Gwo-Bin
Published: January 2021

Optimization of aptamer selection using tissue samples has been carried out on an automated microfluidic system and one screened aptamer exhibited high specificity and affinity towards ovarian cancer tissue.
Plasmonic Cu2−xS nanoparticles: a brief introduction of optical properties and applications
Authors: Chen Lihui , Hu Haifeng , Chen Yuzhou , Gao Jing , Li Guohua
Published: January 2021

LSPR of Cu2−xS and the resulting plasmonic applications are summarized. Remaining open questions and further research directions are proposed.
Polarizability is a key parameter for molecular electronics
Authors: Gillet Angélique , Cher Sébastien , Tassé Marine , Blon Thomas , Alves Sandra , Izzet Guillaume , Chaudret Bruno , Proust Anna , Demont Phillipe , Volatron Florence , Tricard Simon
Published: January 2021

Conducting AFM measurements on self-assemblies of platinum nanoparticles including polyoxometalates show that the polarizability of the molecules is a key parameter to consider electron transport.
Radiative heat transfer at the nanoscale: experimental trends and challenges
Authors: Lucchesi Christophe , Vaillon Rodolphe , Chapuis Pierre-Olivier
Published: January 2021

Beyond the usual surface-to-surface Planck's law of thermal radiation, nanoscale radiative heat transfer is experiencing a revolution.
Theoretical insight into the CdS/FAPbI3 heterostructure: a promising visible-light absorber
Authors: Chang Junli , Jiang Liping , Wang Guangzhao , Huang Yuhong , Chen Hong
Published: January 2021

The optical absorption performance of the perovskite FAPbI3 in the visible-light range is significantly improved by constructing a CdS/FAPbI3 heterostructure.
Multiple stimulus-responsive behavior of a triphenylamine-substituted acylhydrazone derivative
Authors: Qi Hongyang , Bai Binglian , Wang Haitao , Wei Jue , Li Min , Xin Hong
Published: January 2021

A triphenylamine-based acylhydrazone derivative (TBHM) was synthesized, and its emission properties either in solution or in the solid state were studied.
The influence of triphenylamine as a donor group on Zn–porphyrin for dye sensitized solar cell applications
Authors: Kotteswaran S. , Ramasamy P.
Published: January 2021

Novel donor–π–acceptor based Zn–porphyrins (Ko-1 and Ko-2) have been designed and synthesized. Triphenylamine (TPA) was introduced instead of hydrogen in the porphyrin core. A solar cell efficiency was achieved that was almost equal to N719 dye.
Catalytic synthesis of benzannelated macrocyclic di- and triperoxides based on phenols
Authors: Makhmudiyarova Nataliya N. , Ishmukhametova Irina R. , Shangaraev Kamil R. , Dzhemileva Lilya U. , D'yakonov Vladimir A. , Ibragimov Askhat G. , Dzhemilev Usein M.
Published: January 2021

An efficient method for the synthesis of benzannelated macrocyclic di- and triperoxides by cyclocondensation of aromatic compounds with bis-hydroperoxides and formaldehyde in the presence of lanthanide catalysts has been developed.
Defect engineering and characterization of active sites for efficient electrocatalysis
Authors: Yan Xuecheng , Zhuang Linzhou , Zhu Zhonghua , Yao Xiangdong
Published: January 2021

This review highlights recent advancements in defect engineering and characterization of both metal-free carbons and transition metal-based electrocatalysts.
Living cationic polymerization of vinyl ethers initiated by electrophilic selenium reagents under ambient conditions
Authors: Lin Xia , Li Jiajia , Zhang Jiandong , Liu Shaoxiang , Lin Xiaofang , Pan Xiangqiang , Zhu Jian , Zhu Xiulin
Published: January 2021

We present a living cationic polymerization of vinyl ethers utilizing electrophilic selenium reagents as initiators and pentacarbonylbromomanganese (Mn(CO)5Br) as the catalyst.
Recent advances in understanding oxygen evolution reaction mechanisms over iridium oxide
Authors: Naito Takahiro , Shinagawa Tatsuya , Nishimoto Takeshi , Takanabe Kazuhiro
Published: January 2021

Recent spectroscopic and computational studies concerning the oxygen evolution reaction over iridium oxides are reviewed to provide the state-of-the-art understanding of its reaction mechanism.
Highly efficient In2S3/WO3 photocatalysts: Z-scheme photocatalytic mechanism for enhanced photocatalytic water pollutant degradation under visible light irradiation
Authors: Qiu Qingqing , Zhu Peng , Liu Yao , Liang Tongxiang , Xie Tengfeng , Lin Yanhong
Published: January 2021

A Z-scheme system In2S3/WO3 heterojunction was fabricated via a mild hydrothermal method and further applied for photocatalytic degradation of tetracycline (TCH) and Rhodamine B (Rh B) under visible light irradiation.
Building with graphene oxide: effect of graphite nature and oxidation methods on the graphene assembly
Authors: Kim Ji Hoon , Shim Gyu Hyeon , Vo Thi To Nguyen , Kweon Boyeon , Kim Koung Moon , Ahn Ho Seon
Published: January 2021

In this study, the article investigated the effect of starting graphite sources, and oxidation methods on graphene oxide (GO) synthesis and the porous structure of building assembly into thermally expanded graphene oxide (TEGO).
Efficient synthesis of 5-ethoxymethylfurfural from biomass-derived 5-hydroxymethylfurfural over sulfonated organic polymer catalyst
Authors: Xiang Yanping , Wen Sha , Tian Yi , Zhao Kangyu , Guo Dongwen , Cheng Feng , Xu Qiong , Liu Xianxiang , Yin Dulin
Published: January 2021

The sulfonic acid grafted porous organic nitrogen-containing polymer (PDVTA-SO3H) exhibited excellent catalytic activity in the synthesis of 5-ethoxymethylfurfural (EMF) from 5-hydroxymethylfurfural (HMF).
Visible light-driven Giese reaction with alkyl tosylates catalysed by nucleophilic cobalt
Authors: Komeyama Kimihiro , Michiyuki Takuya , Teshima Yoshikazu , Osaka Itaru
Published: January 2021

We report an efficient approach for the Giese reaction with abundant alkyl tosylates as alkyl radical sources using a nucleophilic cobalt catalyst under visible-light irradiation.
Deterministic control of adhesive crack propagation through jamming based switchable adhesives
Authors: Haverkamp Cole B. , Hwang Dohgyu , Lee Chanhong , Bartlett Michael D.
Published: January 2021

We show deterministic control of adhesive crack initiation, propagation, and arresting by integrating a granular jamming layer into adhesive films.
Au integrated AgPt nanorods for the oxygen reduction reaction in proton exchange membrane fuel cells
Authors: Fidiani Elok , Thirunavukkarasu Gnanavel , Li Yang , Chiu Yu-Lung , Du Shangfeng
Published: January 2021

The development of new electrode fabrication approaches from highly active electrocatalysts to replace the state-of-the-art Pt/C is most desirable for enhancing power performance and durability in proton exchange membrane fuel cells.
H-bond/ionic coordination switching for fabrication of highly oriented cellulose hydrogels
Authors: Zhou Shuai , Guo Kechun , Bukhvalov Danil , Zhu Wenzhuo , Wang Jian , Sun Wen , He Ming
Published: January 2021

The H2O/Ca2+ exchange led to a facile switch between dominant H-bond and ionic coordination and achieved a continuous regulation for higher orientation. This unique strategy can be used for programmable design of various anisotropic hydrogels.
Zinc-methacrylate passivation enables an efficient and stable perovskite nanocrystal-polymer composite for LED applications
Authors: Li Dan , Liu Yixuan , Shi Shuangshuang , Li Yanjun , Geng Chong , Xu Shu
Published: January 2021

Zinc methacrylate passivation and crosslinking provide efficient and stable perovskite nanocrystal–PMMA nanocomposite films for light down-conversion applications.
Effect of commensurate lithium doping on the scintillation of two-dimensional perovskite crystals
Authors: Maddalena Francesco , Xie Aozhen , Arramel  , Witkowski Marcin E. , Makowski Michal , Mahler Benoit , Drozdowski Winicjusz , Mariyappan Thambidurai , Springham Stuart Victor , Coquet Philippe , Dujardin Christophe , Birowosuto Muhammad Danang , Dang Cuong
Published: January 2021

Commensurate Lithium doping of two-dimensional lead halide perovskites leads to improved scintillation properties, with enhanced light yield, narrower energy resolution, higher radiation hardness and faster scintillation decay.
First-principles prediction of ferroelasticity tuned anisotropic auxeticity and carrier mobility in two-dimensional AgO
Authors: Zhang Lei , Tang Cheng , Zhang Chunmei , Gu Yuantong , Du Aijun
Published: January 2021

A novel AgO monolayer is highlighted with ferroelasticity tuned anisotropic mechanical and electronic properties.
Opposite actions of two dsRNA-binding proteins PACT and TRBP on RIG-I mediated signaling
Authors: Vaughn Lauren S. , Chukwurah Evelyn , Patel Rekha C.
Published: January 2021

An integral aspect of innate immunity is the ability to detect foreign molecules of viral origin to initiate antiviral signaling via pattern recognition receptors (PRRs). One such receptor is the RNA helicase retinoic acid inducible gene 1 (RIG-I), which detects and is activated by 5′triphosphate uncapped double stranded RNA (dsRNA) as well as the cytoplasmic viral mimic dsRNA polyI:C. Once activated, RIG-I's CARD domains oligomerize and initiate downstream signaling via mitochondrial antiviral signaling protein (MAVS), ultimately inducing interferon (IFN) production. Another dsRNA binding protein PACT, originally identified as the cellular protein activator of dsRNA-activated protein kinase (PKR), is known to enhance RIG-I signaling in response to polyI:C treatment, in part by stimulating RIG-I's ATPase and helicase activities. TAR-RNA-binding protein (TRBP), which is ∼45% homologous to PACT, inhibits PKR signaling by binding to PKR as well as by sequestration of its’ activators, dsRNA and PACT. Despite the extensive homology and similar structure of PACT and TRBP, the role of TRBP has not been explored much in RIG-I signaling. This work focuses on the effect of TRBP on RIG-I signaling and IFN production. Our results indicate that TRBP acts as an inhibitor of RIG-I signaling in a PACT- and PKR-independent manner. Surprisingly, this inhibition is independent of TRBP's post-translational modifications that are important for other signaling functions of TRBP, but TRBP's dsRNA-binding ability is essential. Our work has major implications on viral susceptibility, disease progression, and antiviral immunity as it demonstrates the regulatory interplay between PACT and TRBP IFN production.
WITHDRAWN: Epithelial to Mesenchymal Transition (EMT) Regulated by MicroRNA-429 in Pancreatic Ductal Adenocarcinoma (PDAC)
Authors: Zheng Gang , Wang Zhigang , Tang Jie , Sun Yin , Hu Zhou , Chen Huiping
Published: January 2021

The Accepted Manuscript version of this article (published on 18 January 2021) was withdrawn on 29 January 2021 due to concerns over authorship.