bluuebunny/crossref_metadata_2025 huggingface dataset:
Laser Dissimilar Joining of Al7075T6 with Glass-Fiber-Reinforced Polyamide Composite
Authors: Ukar Eneko , Arrizubieta Jon Iñaki , Ferros Mercedes , Andres Maite , Liebana Fernando
Published: January 2020

Dissimilar joining between metal and composite sheets is usually carried out by mechanical or adhesive joining. Laser dissimilar joining between metal and composite sheets could be an alternative to these methods, as it is a cost-effective and versatile joining technique. Previously, textured metallic and composite parts have been held together and heated with a laser beam while pressure is applied to allow the melted polymer to flow into the cavities of the metal part. The main issue of this process relates to reaching the same joint strength repetitively with appropriate process parameters. In this work, both initial texturing and laser joining parameters are studied for Al 7075-T6 and glass-fiber-reinforced PA6 composite. A groove-based geometry was studied in terms of depth-to-width aspect ratio to find an optimal surface using a nanosecond fiber laser for texturing. Laser joining parameters were also studied with different combinations of surface temperature, heating strategy, pressure, and laser feed rate. The results are relatively good for grooves with aspect ratios from 0.94 to 4.15, with the widths of the grooves being the most critical factor. In terms of joining parameters, surface reference temperature was found to be the most influential parameter. Underheating does not allow correct material flow in textured cavities, while overheating also causes high dispersion in the resulting shear strength. When optimal parameters are applied using correct textures, shear strength values over 26 kN are reached, with a contact area of 35 × 45 mm2.
IRI Performance Models for Flexible Pavements in Two-Lane Roads until First Maintenance and/or Rehabilitation Work
Authors: Pérez-Acebo Heriberto , Linares-Unamunzaga Alaitz , Rojí Eduardo , Gonzalo-Orden Hernán
Published: January 2020

Pavement performance models play a vital role in any pavement management system. The Regional Government of Biscay (RGB) (Spain) manages a 1200 km road network and conducts pavement data collections, including the International Roughness Index (IRI) values. The aim of the paper is to develop an IRI performance model for two-lane roads with flexible pavement until the first maintenance and/or rehabilitation activity is performed. Due to the huge amount of available information, a deterministic model was selected. A literature review of deterministic models showed that, apart from age and traffic volumes, the pavement structure is a key factor. Therefore, it was decided to analyze the only road stretches whose entire pavement section was known (surface layer + base + subbase). Various variables related to age, traffic volumes and employed materials were introduced as possible factors. The multiple linear regression model with the highest coefficient of determination and all the variables significant included the real pavement age, the cumulated heavy traffic and the total thickness of bituminous layers. As the material employed in the surface layer could affect roughness progression, a qualitative variable was introduced to consider various surface materials. The model improved its accuracy, indicating that the surface layer material is also an influencing factor on IRI evolution.
A Simple Protocol for Certifying Graph States and Applications in Quantum Networks
Authors: Markham Damian , Krause Alexandra
Published: January 2020

We present a simple protocol for certifying graph states in quantum networks using stabiliser measurements. The certification statements can easily be applied to different protocols using graph states. We see, for example, how it can be used for measurement based verified quantum computation, certified sampling of random unitaries, quantum metrology and sharing quantum secrets over untrusted channels.
Selective Area Epitaxy of GaAs/Ge/Si Nanomembranes: A Morphological Study
Authors: Bollani Monica , Fedorov Alexey , Albani Marco , Bietti Sergio , Bergamaschini Roberto , Montalenti Francesco , Ballabio Andrea , Miglio Leo , Sanguinetti Stefano
Published: January 2020

We demonstrate the feasibility of growing GaAs nanomembranes on a plastically-relaxed Ge layer deposited on Si (111) by exploiting selective area epitaxy in MBE. Our results are compared to the case of the GaAs homoepitaxy to highlight the criticalities arising by switching to heteroepitaxy. We found that the nanomembranes evolution strongly depends on the chosen growth parameters as well as mask pattern. The selectivity of III-V material with respect to the SiO2 mask can be obtained when the lifetime of Ga adatoms on SiO2 is reduced, so that the diffusion length of adsorbed Ga is high enough to drive the Ga adatoms towards the etched slits. The best condition for a heteroepitaxial selective area epitaxy is obtained using a growth rate equal to 0.3 ML/s of GaAs, with a As BEP pressure of about 2.5 × 10−6 torr and a temperature of 600 °C.
Acknowledgement to Reviewers of Energies in 2019
Authors: Energies Editorial Office
Published: January 2020

The editorial team greatly appreciates the reviewers who have dedicated their considerable time and expertise to the journal’s rigorous editorial process over the past 12 months[...]
Exergy Analysis of Gas Switching Chemical Looping IGCC Plants
Authors: Arnaiz del Pozo Carlos , Jiménez Álvaro Ángel , Cloete Jan Hendrik , Cloete Schalk , Amini Shahriar
Published: January 2020

Integrated gasification combined cycles (IGCC) are promising power production systems from solid fuels due to their high efficiency and good environmental performance. Chemical looping combustion (CLC) is an effective route to reduce the energy penalty associated with CO2 capture. This concept comprises a metal oxygen carrier circulated between a reduction reactor, where syngas is combusted, and an oxidation reactor, where O2 is withdrawn from an air stream. Parallel to CLC, oxygen carriers that are capable of releasing free O2 in the reduction reactor, i.e., chemical looping oxygen production (CLOP), have been developed. This offers interesting integration opportunities in IGCC plants, replacing energy demanding air separation units (ASU) with CLOP. Gas switching (GS) reactor cluster technology consists of a set of reactors operating in reduction and oxidation stages alternatively, providing an averaged constant flow rate to the gas turbine and a CO2 stream readily available for purification and compression, and avoiding the transport of solids across reactors, which facilitates the scale up of this technology at pressurized conditions. In this work, exergy analyses of a gas switching combustion (GSC) IGCC plant and a GSOP–GSC IGCC plant are performed and consistently benchmarked against an unabated IGCC and a precombustion CO2 capture IGCC plant. Through the exergy analysis methodology, an accurate assessment of the irreversible loss distribution in the different power plant sections from a second-law perspective is provided, and new improvement pathways to utilize the exergy contained in the GSC reduction gases outlet are identified.
Optimal Phase Arrangement of Distribution Transformers for System Unbalance Improvement and Loss Reduction
Authors: Tu Chia-Sheng , Tsai Ming-Tang
Published: January 2020

This paper presents an efficient strategy for transformer planning to reduce the system losses by means of transformer rearrangement. The customer connected to the distribution transformer are first investigated by the field survey, and the loads of the various customers are collected from the customer information system (CIS) and distribution database system (DAS) to derive their load patterns. The objective function is to minimize the total line loss in the 24 intervals. An improved bacterial foraging algorithm (IBFO) is proposed herein to find the optimal phase combination of distribution transformers to minimize the total line loss by considering operating constraints. A three-phase load flow program with Eeuivalent current injection (ECT) is used to solve the total line loss and system unbalance factor on a Taipower distribution system. The results can help operators not only perform the proper installation phase selection of distribution transformers, but also reduce the system losses, decrease the system unbalance factor, and improve the voltage profiles of the buses.
Modelling and Numerical Simulation for an Innovative Compound Solar Concentrator: Thermal Analysis by FEM Approach
Authors: Carlini Maurizio , McCormack Sarah Josephine , Castellucci Sonia , Ortega Anita , Rotondo Mirko , Mennuni Andrea
Published: January 2020

The work presents a heat transfer analysis carried out with the use of COMSOL Multiphysics software applied to a new solar concentrator, defined as the Compound Parabolic Concentrator (CPC) system. The experimental measures have been conducted for a truncated CPC prototype system with a half-acceptance angle of 60°, parabola coefficient of 4 m−1 and four solar cells in both covered and uncovered configurations. These data are used to validate the numerical scenario, to be able to use the simulations for different future systems and works. The second challenge has been to change the reflector geometry, the half-acceptance angle (60° ÷ 75°) and the parabola coefficient (3 m−1 ÷ 6 m−1) to enhance the concentration of sun rays on the solar cells. The results show that the discrepancy between experimental data and COMSOL Multiphysics (CM) have led to validate the scenarios considering the average temperature on the solar cells. These scenarios are used for the parametric analysis, observing that the optimal geometry for the higher power and efficiency of the whole system is reached with a lower half-acceptance angle and parabola coefficient.
Improved Faulted Phase Selection Algorithm for Distance Protection under High Penetration of Renewable Energies
Authors: Martínez Carrasco Eduardo , Comech Moreno María Paz , Villén Martínez María Teresa , Borroy Vicente Samuel
Published: January 2020

The high penetration of renewable energies will affect the performance of present protection algorithms due to fault current injection from generators based on power electronics. This paper explains the process followed for analyzing this effect on distance protection and the development of a new algorithm that improves its performance in such a scenario. First of all, four commercial protection relays were tested before fault current contribution from photovoltaic system and full converter wind turbines using the hardware in the loop technique. The analysis of results obtained, jointly with a theoretical analysis based on commonly used protection strategy of superimposed quantities, lead to a conclusion about the cause of observed wrong behaviors of present protection algorithms under a high penetration of renewables. According to these conclusions, a new algorithm has been developed to improve the detection of faulted phase selection and directionality on distance protection under a short circuit current fed by renewable energy sources.
Estimation of Carbon Dioxide Emissions from a Diesel Engine Powered by Lignocellulose Derived Fuel for Better Management of Fuel Production
Authors: Tucki Karol , Orynycz Olga , Wasiak Andrzej , Świć Antoni , Mruk Remigiusz , Botwińska Katarzyna
Published: January 2020

Managing of wastes rich in lignocellulose creates the opportunity to produce biofuels that are in full compliance with the principles of sustainable development. Biomass, as a suitable base for the production of biofuels, does not have to be standardized, and its only important feature is the appropriate content of lignocellulose, which assures great freedom in the selection of input. Biobutanol, obtained from this type of biomass, can be used as fuel for internal combustion engines, including diesel engines. In the era of strict environmental protection regulations, especially concerning atmospheric air, any new fuel, apart from good energetic properties, should also show beneficial ecological effects. This study investigates the carbon dioxide emissions from biobutanol powered diesel engine by means of use of the simulation model. The parameters of a real passenger car powered by a diesel engine were used for simulation carried out accordingly to the WLTP (Worldwide Harmonized Light Vehicle Test Procedure) approval procedure as the current test for newly manufactured cars. The results obtained for biobutanol were compared with simulated exhaust emissions obtained for conventional diesel and with FAME (fatty acid methyl esters)—the most popular biofuel. Biobutanol, in spite of its higher consumption, showed lower direct carbon dioxide emissions than both: the conventional diesel and FAME. In addition, a LCA (life cycle assessment) was carried out for the fuels and vehicles in question using the SimaPro package. Therefore, the implementation of butyl alcohol as a fuel provides favorable environmental effects. This result gives arguments towards biofuel production management indicating that implementation of biobutanol production technology mitigates carbon dioxide emission, as well as promotes lignocellulosic resources rather than edible parts of the plants.
MODIS-Derived Estimation of Soil Respiration within Five Cold Temperate Coniferous Forest Sites in the Eastern Loess Plateau, China
Authors: Yan Junxia , Zhang Xue , Liu Ju , Li Hongjian , Ding Guangwei
Published: January 2020

Soil respiration (Rs) is seldom analyzed using remotely sensed data because satellite technology has difficulty monitoring various respiratory processes in the soil. We investigated the potential of remote sensing data products to estimate Rs, including land surface temperature (LST) and spectral vegetation indices from the Moderate Resolution Imaging Spectroradiometer (MODIS), using a nine-year (2007–2015) field measurement dataset of Rs and soil temperature (Ts) at five forest sites at the eastern Loess Plateau, China. The results indicate that soil temperature is the primary factor influencing the seasonal variation of Rs at the five sites. The accuracy of the model based on the observed data is not significantly different from the model based on MODIS-derived nighttime LST values. There was a significant difference with the model based on MODIS-derived daytime LST values. Therefore, nighttime LST was the optimum LST for estimation of Rs. The normalized difference vegetation index (NDVI) consistently exhibited a stronger correlation with Rs when compared to the green edge chlorophyll index and enhanced vegetation index. Further analysis showed that adding the NDVI into the model considering only Ts or nighttime LST could significantly improve the simulation accuracy of Rs. The models depending on nighttime LST and NDVI showed comparable accuracy with the models based on the in situ Ts and NDVI. These results suggest that models based entirely on remote sensing data from MODIS have the potential to estimate Rs at the cold temperate coniferous forest sites. The performance of the model in other vegetation types or regions has also been proved. Our conclusions further confirmed that it is feasible for large-scale estimates of Rs by means of MODIS data in temperate coniferous forest ecosystems.
Variation in Foliar ẟ15N Reflects Anthropogenic Nitrogen Absorption Potential of Mangrove Forests
Authors: Tanu Ferdouse Zaman , Asakura Yasuhiro , Takahashi Satoshi , Hinokidani Ko , Nakanishi Yasuhiro
Published: January 2020

Research Highlights: Mangrove forests are absorbing anthropogenically produced excess nitrogen under moderate to intensive human interaction in the study sites, further indicating the degree of deviation from the natural ecosystem condition. Background and Objectives: Mangrove species, when directly connected to anthropogenic activities such as sewage disposal, agricultural inputs, and receiving of animal manure, absorb excess nutrients from the systems and act as ecological indicators of long-term natural changes. However, there is a paucity of examples of how the mangroves respond to a land-use gradient comparing to the non-mangrove plants under indirect anthropogenic impacts. Materials and Methods: In this investigation, foliar total nitrogen (N), carbon to nitrogen (C/N) ratio, and δ15N of mangrove and non-mangrove species collected from 15 watersheds on three islands in Okinawa, Japan, have been compared. The land-use areas in the study watersheds were delineated by ArcGIS software, and the correlation between the foliar traits and the human-affected area ratios were examined. Results: Foliar δ15N of the mangroves, which was significantly different from those of the non-mangroves on each island, showed significantly higher values (5‰ to 14‰) in human-affected forests, whereas the values were up to 3‰ in pristine forests. Furthermore, the significant positive relationship between foliar nitrogenous traits and the human-affected area ratios suggested that the anthropogenic N might be regulating foliar N content and δ15N signature on the sites. Conclusion: Different degrees of foliar isotopic fractionation with the land-use gradient have clarified that mangroves can be a powerful tool for monitoring ecosystem conditions under anthropogenic disturbances.
Factors Associated with Seasonal Food Insecurity among Small-Scale Subsistence Farming Households in Rural Honduras
Authors: Dodd Warren , Gómez Cerna Marvin , Orellena Paola , Humphries Sally , Sadoine Margaux L. , Zombré David , Zinszer Kate , Kipp Amy , Cole Donald C.
Published: January 2020

In the context of climate change, a nutritional transition, and increased pressures to migrate internally and internationally, this study examined the relationship between seasonal food insecurity and demographic, socioeconomic, and agricultural production factors among small-scale subsistence farmers in rural northern Honduras. Anchored by a partnership with the Fundación para la Investigación Participativa con Agricultores de Honduras (FIPAH) and the Yorito Municipal Health Centre, a cross-sectional household survey was administered in Yorito, Honduras, in July 2014. The study population included 1263 individuals from 248 households across 22 rural communities. A multivariate mixed effects negative binomial regression model was built to investigate the relationship between the self-reported number of months without food availability and access from subsistence agriculture in the previous year (August 2013–July 2014) and demographic, socioeconomic, and agricultural production variables. This study found a lengthier ‘lean season’ among surveyed household than previously documented in Honduras. Overall, 62.2% (95% confidence interval (CI): [59.52, 64.87]) of individuals experienced at least four months of insufficient food in the previous year. Individuals from poorer and larger households were more likely to experience insufficient food compared to individuals from wealthier and smaller households. Additionally, individuals from households that produced both maize and beans were less likely to have insufficient food compared to individuals from households that did not grow these staple crops (prevalence ratio (PR) = 0.83; 95% CI: [0.69, 0.99]). Receiving remittances from a migrant family member did not significantly reduce the prevalence of having insufficient food. As unpredictable crop yields linked to climate change and extreme weather events are projected to negatively influence the food security and nutrition outcomes of rural populations, it is important to understand how demographic, socioeconomic, and agricultural production factors may modify the ability of individuals and households engaged in small-scale subsistence agriculture to respond to adverse shocks.
T1ρ-Mapping for Musculoskeletal Pain Diagnosis: Case Series of Variation of Water Bound Glycosaminoglycans Quantification before and after Fascial Manipulation® in Subjects with Elbow Pain
Authors: Menon Rajiv , Oswald Stephen , Raghavan Preeti , Regatte Ravinder , Stecco Antonio
Published: January 2020

Diagnosis and management of musculoskeletal pain is a major clinical challenge. Following this need, the first aim of our study was to provide an innovative magnetic resonance technique called T1ρ to quantify possible alterations in elbow pain, a common musculoskeletal pain syndrome that has not a clear etiology. Five patients were recruited presenting chronic elbow pain (>3 months), with an age between 30 and 70 years old. Patients underwent two T1ρ-mapping evaluations, one before and one after the series of Fascial Manipulation® (FM) treatments. After the first MRI evaluation, a Disability of the Arm, Shoulder and Hand (DASH) questionnaire was administered to quantify the symptoms and pain intensity. Patients then received three sessions of FM, once a week for 40 min each. A statistically significant difference was found between bound and unbound water concentration before and after FM treatment. Our preliminary data suggest that the application of the manual method seems to decrease the concentration of unbound water inside the deep fascia in the most chronic patients. This could explain the change in viscosity perceived by many practitioners as well as the decrease of symptoms due to the restoration of the normal property of the loose connective tissue. Being able to identify an altered deep fascial area may better guide therapies, contributing to a more nuanced view of the mechanisms of pain.
Horticultural Therapy Program for People with Mental Illness: A Mixed-Method Evaluation
Authors: Siu Andrew M.H. , Kam Michael , Mok Ide
Published: January 2020

Background. Horticultural therapy (HT) has long been used in the rehabilitation of people with mental illness, but many HT programs are not standardized, and there have been few evaluation studies. Aims. This study evaluated the process and outcomes of a standardized horticultural program using a mixed methodology, i.e., systematic integration (“mixing”) of quantitative and qualitative data within a study. Methods. Participants who have mental illnesses were assigned to a treatment (HT) and a comparison group (n = 41 for each group). The process and outcomes of the program, including stress and anxiety, engagement and participation, affect changes, mental well-being, and social exchange, were obtained using self-completed questionnaires, observational ratings of participants during the group, as well as through a focus group. Results. The study results supported the proposal HT is effective in increasing mental well-being, engagement, and the sense of meaningfulness and accomplishment of participants. Many participants reported a reduction in stress and anxiety in the focus group, but positive changes in affect were not fully observed during the group process or captured by quantitative measures. The participants also did not report increases in the social exchange over the HT sessions. Conclusion. The evidence supports that HT is effective in increasing mental well-being, engagement in meaningful activities, but did not result in significant affect changes during therapy, or increase social exchanges among people with mental illness.
Reducing Sedentary Time among Older Adults in Assisted Living: Perceptions, Barriers, and Motivators
Authors: Voss M. Lauren , Pope J. Paige , Copeland Jennifer L.
Published: January 2020

Older adults accumulate more sedentary time (ST) than any other age group, especially those in assisted living residences (ALRs). Reducing prolonged ST could help maintain function among older adults. However, to develop effective intervention strategies, it is important to understand the factors that influence sedentary behavior. The purpose of this study was to explore perceptions of ST as well as barriers and motivators to reducing ST among older adults in assisted living, in the context of the Social Ecological Model (SEM). Using a qualitative description approach, we sought to learn about participants’ perceptions of sedentary time in their daily lives. Semi-structured focus groups were held at six ALRs with 31 participants (84% women, 83.5 ± 6.5 years). Data were transcribed and coded using an inductive thematic approach. Themes were categorized based on four levels of the SEM: individual, social, physical environment, and organization. Many reported barriers were at the individual level (e.g., lack of motivation, pain, fatigue) while others were associated with the organization or social environment (e.g., safety concerns, lack of activities outside of business hours, and social norms). These findings suggest that there are unique challenges and opportunities to consider when designing ST interventions for assisted living.
Environmental Exposure of Arsenic in Groundwater Associated to Carcinogenic Risk in Underweight Children Exposed to Fluorides
Authors: Molina-Frechero Nelly , Nevarez-Rascón Martina , Tremillo-Maldonado Omar , Vergara-Onofre Marcela , Gutiérrez-Tolentino Rey , Gaona Enrique , Castañeda Enrique , Jarquin-Yañez Lizet , Bologna-Molina Ronell
Published: January 2020

Background: The purpose of this study was to determine the concentration of inorganic arsenic (As) in the potable water available to the population to be able to estimate the non-carcinogenic risks for underweight children and the carcinogenic risk for adults exposed to As intake who live in the Mezquital municipality, Durango, Mexico. Methods: The As content was quantifed in the water supply sources for human use and its intake was estimated in Mezquital population, southern Durango. With the data obtained, the hazard quotient (HQ) was calculated to determine the non-carcinogenic risk to develop chronic systemic effects in underweight children. The Environmental Protection Agency (EPA) reference health values estimating As exposure risk are from 0.0003 mg/kg/day (non-carcinogenic) to 1.5 mg/kg/day (carcinogenic risk). Results: The analyzed waters presented as concentrations that varied from 0.3 to 10.2 µg/L, with a mean of 7.35 µg/L (CI 95% 6.27–8.38). The exposure dose was 0.4 to 1.36, and the HQ was 1.90 to 6.48 mg/kg/day, the estimated carcinogenic risk from adults varied from 1.28 to 4.37E−4, with values of 3.74–4.37E−4 mg/kg/day in central area. Conclusions: The children are at risk to develop chronic systemic effects due to ingestion of As from water.
Maturity Related Differences in Body Composition Assessed by Classic and Specific Bioimpedance Vector Analysis among Male Elite Youth Soccer Players
Authors: Toselli Stefania , Marini Elisabetta , Maietta Latessa Pasqualino , Benedetti Luca , Campa Francesco
Published: January 2020

The aim of this study was to analyze the efficiency of classic and specific bioelectrical impedance vector analysis (BIVA) in the assessment of maturity related differences in body composition among male elite youth soccer players, and to provide bioelectrical impedance reference data for this category. A group of 178 players (aged 12.1 ± 1.6 years) were registered in a professional Italian soccer team participating in the first division (Serie A). They were divided into three groups according to their maturity status while bioelectrical resistance and reactance were obtained. The classic and specific BIVA procedures were applied, which correct bioelectrical values for body height and body geometry, respectively. Percentage of fat mass (FM%) and total body water (TBW (L)) were estimated from bioelectrical values. Age-specific z-scores of the predicted age at peak height velocity identified 29 players as earlier-, 126 as on time-, and 23 as later-maturing. TBW was higher (p < 0.01) in adolescents classified as “early” maturity status compared to the other two groups and classic BIVA confirmed these results. Conversely, no differences in FM% were found among the groups. Specific vector length showed a higher correlation (r = 0.748) with FM% compared with the classic approach (r = 0.493). Classic vector length showed a stronger association (r = −0.955) with TBW compared with specific (r = −0.263). Specific BIVA turns out to be accurate for the analysis of FM% in athletes, while classic BIVA shows to be a valid approach to evaluate TBW. An original data set of bioelectric impedance reference values of male elite youth soccer players was provided.
Netizens’ Food Safety Knowledge, Attitude, Behaviors, and Demand for Science Popularization by WeMedia
Authors: Zhao Yong , Yu Xinyang , Xiao Yangxue , Cai Zhengjie , Luo Xinmiao , Zhang Fan
Published: January 2020

This study aimed to investigate netizens’ food safety knowledge, attitudes and behavior, status, and their demand for science popularization by WeMedia. Firstly, participants were recruited by WeMedia, including WeChat, Microblog, and QQ. Then, a web-based survey was conducted using a self-designed questionnaire, which comprised 29 items about the knowledge, attitude, and practice (KAP) status of netizens’ food safety and the demand for science popularization by WeMedia. A correct answer on knowledge-related items was assigned a value of 1 point, and the high, medium, and low levels of knowledge had a total of 6–8, 3–5, and 0–2 points, respectively. A correct answer on attitude-related items was given a value of 1 to 5 points, and the high, medium, and low levels of attitude had a total of 16–20, 8–16, and l4–8 points, respectively. A correct answer on practice-related items was given a value of 5 to 1 point, and the high, medium, and low levels of attitude had a total of 22–30, 14–22, and 6–14 points, respectively. Results showed that the distribution of the different levels of the KAP scores were as follows: high (79.0%), medium (20.2%), low (0.8%); high (65.6%), medium (34.1%), low (0.3%); high (70.1%), medium (29.4%), low (0.5%). Approximately 86% of the subjects desired to obtain food safety knowledge from WeMedia. In conclusion, the netizens’ KAP in food safety are relatively optimistic. A large demand for science popularization on food safety knowledge by WeMedia is warranted. The WeMedia has a potentially important role in science popularization and health promotion related to food safety and health behaviors.
Advanced Technology Use by Care Professionals
Authors: Brandsma Tom , Stoffers Jol , Schrijver Ilse
Published: January 2020

Advanced technology is a primary solution for the shortage of care professionals and increasing demand for care, and thus acceptance of such technology is paramount. This study investigates factors that increase use of advanced technology during elderly care, focusing on current use of advanced technology, factors that influence its use, and care professionals’ experiences with the use. This study uses a mixed-method design. Logfiles were used (longitudinal design) to determine current use of advanced technology, questionnaires assessed which factors increase such use, and in-depth interviews were administered to retrieve care professionals’ experiences. Findings suggest that 73% of care professionals use advanced technology, such as camera monitoring, and consult clients’ records electronically. Six of nine hypotheses tested in this study were supported, with correlations strongest between performance expectancy and attitudes toward use, attitudes toward use and satisfaction, and effort expectancy and performance expectancy. Suggested improvements for advanced technology include expanding client information, adding report functionality, solving log-in problems, and increasing speed. Moreover, the quickest way to increase acceptance is by improving performance expectancy. Care professionals scored performance expectancy of advanced technology lowest, though it had the strongest effect on attitudes toward the technology.
High Mobility Group A (HMGA): Chromatin Nodes Controlled by a Knotty miRNA Network
Authors: Sgarra Riccardo , Pegoraro Silvia , D’Angelo Daniela , Ros Gloria , Zanin Rossella , Sgubin Michela , Petrosino Sara , Battista Sabrina , Manfioletti Guidalberto
Published: January 2020

High mobility group A (HMGA) proteins are oncofoetal chromatin architectural factors that are widely involved in regulating gene expression. These proteins are unique, because they are highly expressed in embryonic and cancer cells, where they play a relevant role in cell proliferation, stemness, and the acquisition of aggressive tumour traits, i.e., motility, invasiveness, and metastatic properties. The HMGA protein expression levels and activities are controlled by a connected set of events at the transcriptional, post-transcriptional, and post-translational levels. In fact, microRNA (miRNA)-mediated RNA stability is the most-studied mechanism of HMGA protein expression modulation. In this review, we contribute to a comprehensive overview of HMGA-targeting miRNAs; we provide detailed information regarding HMGA gene structural organization and a comprehensive evaluation and description of HMGA-targeting miRNAs, while focusing on those that are widely involved in HMGA regulation; and, we aim to offer insights into HMGA-miRNA mutual cross-talk from a functional and cancer-related perspective, highlighting possible clinical implications.
In-silico Analysis of NF1 Missense Variants in ClinVar: Translating Variant Predictions into Variant Interpretation and Classification
Authors: Accetturo Matteo , Bartolomeo Nicola , Stella Alessandro
Published: January 2020

Background: With the advent of next-generation sequencing in genetic testing, predicting the pathogenicity of missense variants represents a major challenge potentially leading to misdiagnoses in the clinical setting. In neurofibromatosis type 1 (NF1), where clinical criteria for diagnosis may not be fully present until late infancy, correct assessment of variant pathogenicity is fundamental for appropriate patients’ management. Methods: Here, we analyzed three different computational methods, VEST3, REVEL and ClinPred, and after extracting predictions scores for 1585 NF1 missense variants listed in ClinVar, evaluated their performances and the score distribution throughout the neurofibromin protein. Results: For all the three methods, no significant differences were present between the scores of “likely benign”, “benign”, and “likely pathogenic”, “pathogenic” variants that were consequently collapsed into a single category. The cutoff values for pathogenicity were significantly different for the three methods and among benign and pathogenic variants for all methods. After training five different models with a subset of benign and pathogenic variants, we could reclassify variants in three sharply separated categories. Conclusions: The recently developed metapredictors, which integrate information from multiple components, after gene-specific fine-tuning, could represent useful tools for variant interpretation, particularly in genetic diseases where a clinical diagnosis can be difficult.
Phytocannabinoids in Neurological Diseases: Could They Restore a Physiological GABAergic Transmission?
Authors: Cifelli Pierangelo , Ruffolo Gabriele , De Felice Eleonora , Alfano Veronica , van Vliet Erwin Alexander , Aronica Eleonora , Palma Eleonora
Published: January 2020

γ-Aminobutyric acid type A receptors (GABAARs) are the main inhibitory mediators in the central nervous system (CNS). GABAARs are pentameric ligand gated ion channels, and the main subunit composition is usually 2α2βγ, with various isotypes assembled within a set of 19 different subunits. The inhibitory function is mediated by chloride ion movement across the GABAARs, activated by synaptic GABA release, reducing neuronal excitability in the adult CNS. Several studies highlighted the importance of GABA-mediated transmission during neuro-development, and its involvement in different neurological and neurodevelopmental diseases, from anxiety to epilepsy. However, while it is well known how different classes of drugs are able to modulate the GABAARs function (benzodiazepines, barbiturates, neurosteroids, alcohol), up to now little is known about GABAARs and cannabinoids interaction in the CNS. Endocannabinoids and phytocannabinoids are lately emerging as a new class of promising drugs for a wide range of neurological conditions, but their safety as medication, and their mechanisms of action are still to be fully elucidated. In this review, we will focus our attention on two of the most promising molecules (Δ9-tetrahydrocannabinol; Δ9-THC and cannabidiol; CBD) of this new class of drugs and their possible mechanism of action on GABAARs.
The Influence of Inflammation on Anemia in CKD Patients
Authors: Gluba-Brzózka Anna , Franczyk Beata , Olszewski Robert , Rysz Jacek
Published: January 2020

Anemia is frequently observed in the course of chronic kidney disease (CKD) and it is associated with diminishing the quality of a patient’s life. It also enhances morbidity and mortality and hastens the CKD progression rate. Patients with CKD frequently suffer from a chronic inflammatory state which is related to a vast range of underlying factors. The results of studies have demonstrated that persistent inflammation may contribute to the variability in Hb levels and hyporesponsiveness to erythropoietin stimulating agents (ESA), which are frequently observed in CKD patients. The understanding of the impact of inflammatory cytokines on erythropoietin production and hepcidin synthesis will enable one to unravel the net of interactions of multiple factors involved in the pathogenesis of the anemia of chronic disease. It seems that anti-cytokine and anti-oxidative treatment strategies may be the future of pharmacological interventions aiming at the treatment of inflammation-associated hyporesponsiveness to ESA. The discovery of new therapeutic approaches towards the treatment of anemia in CKD patients has become highly awaited. The treatment of anemia with erythropoietin (EPO) was associated with great benefits for some patients but not all.
Effects of Dietary Anaplerotic and Ketogenic Energy Sources on Renal Fatty Acid Oxidation Induced by Clofibrate in Suckling Neonatal Pigs
Authors: Lin Xi , Pike Brandon , Zhao Jinan , Fan Yu , Zhu Yongwen , Zhang Yong , Wang Feng , Odle Jack
Published: January 2020

Maintaining an active fatty acid metabolism is important for renal growth, development, and health. We evaluated the effects of anaplerotic and ketogenic energy sources on fatty acid oxidation during stimulation with clofibrate, a pharmacologic peroxisome proliferator-activated receptor α (PPARα) agonist. Suckling newborn pigs (n = 72) were assigned into 8 dietary treatments following a 2 × 4 factorial design: ± clofibrate (0.35%) and diets containing 5% of either (1) glycerol-succinate (GlySuc), (2) tri-valerate (TriC5), (3) tri-hexanoate (TriC6), or (4) tri-2-methylpentanoate (Tri2MPA). Pigs were housed individually and fed the iso-caloric milk replacer diets for 5 d. Renal fatty acid oxidation was measured in vitro in fresh tissue homogenates using [1-14C]-labeled palmitic acid. The oxidation was 30% greater in pig received clofibrate and 25% greater (p < 0.05) in pigs fed the TriC6 diet compared to those fed diets with GlySuc, TriC5, and Tri2MPA. Addition of carnitine also stimulated the oxidation by twofold (p < 0.05). The effects of TriC6 and carnitine on palmitic acid oxidation were not altered by clofibrate stimulation. However, renal fatty acid composition was altered by clofibrate and Tri2MPA. In conclusion, modification of anaplerosis or ketogenesis via dietary substrates had no influence on in vitro renal palmitic acid oxidation induced by PPARα activation.
Elucidating the H+ Coupled Zn2+ Transport Mechanism of ZIP4; Implications in Acrodermatitis Enteropathica
Authors: Hoch Eitan , Levy Moshe , Hershfinkel Michal , Sekler Israel
Published: January 2020

Cellular Zn2+ homeostasis is tightly regulated and primarily mediated by designated Zn2+ transport proteins, namely zinc transporters (ZnTs; SLC30) that shuttle Zn2+ efflux, and ZRT-IRT-like proteins (ZIPs; SLC39) that mediate Zn2+ influx. While the functional determinants of ZnT-mediated Zn2+ efflux are elucidated, those of ZIP transporters are lesser understood. Previous work has suggested three distinct molecular mechanisms: (I) HCO3− or (II) H+ coupled Zn2+ transport, or (III) a pH regulated electrodiffusional mode of transport. Here, using live-cell fluorescent imaging of Zn2+ and H+, in cells expressing ZIP4, we set out to interrogate its function. Intracellular pH changes or the presence of HCO3− failed to induce Zn2+ influx. In contrast, extracellular acidification stimulated ZIP4 dependent Zn2+ uptake. Furthermore, Zn2+ uptake was coupled to enhanced H+ influx in cells expressing ZIP4, thus indicating that ZIP4 is not acting as a pH regulated channel but rather as an H+ powered Zn2+ co-transporter. We further illustrate how this functional mechanism is affected by genetic variants in SLC39A4 that in turn lead to Acrodermatitis enteropathica, a rare condition of Zn2+ deficiency.
NTRK Fusions in Central Nervous System Tumors: A Rare, but Worthy Target
Authors: Gambella Alessandro , Senetta Rebecca , Collemi Giammarco , Vallero Stefano Gabriele , Monticelli Matteo , Cofano Fabio , Zeppa Pietro , Garbossa Diego , Pellerino Alessia , Rudà Roberta , Soffietti Riccardo , Fagioli Franca , Papotti Mauro , Cassoni Paola , Bertero Luca
Published: January 2020

The neurotrophic tropomyosin receptor kinase (NTRK) genes (NTRK1, NTRK2, and NTRK3) code for three transmembrane high-affinity tyrosine-kinase receptors for nerve growth factors (TRK-A, TRK-B, and TRK-C) which are mainly involved in nervous system development. Loss of function alterations in these genes can lead to nervous system development problems; conversely, activating alterations harbor oncogenic potential, promoting cell proliferation/survival and tumorigenesis. Chromosomal rearrangements are the most clinically relevant alterations of pathological NTRK activation, leading to constitutionally active chimeric receptors. NTRK fusions have been detected with extremely variable frequencies in many pediatric and adult cancer types, including central nervous system (CNS) tumors. These alterations can be detected by different laboratory assays (e.g., immunohistochemistry, FISH, sequencing), but each of these approaches has specific advantages and limitations which must be taken into account for an appropriate use in diagnostics or research. Moreover, therapeutic targeting of this molecular marker recently showed extreme efficacy. Considering the overall lack of effective treatments for brain neoplasms, it is expected that detection of NTRK fusions will soon become a mainstay in the diagnostic assessment of CNS tumors, and thus in-depth knowledge regarding this topic is warranted.
Inter-Individual Nectar Chemistry Changes of Field Scabious, Knautia arvensis
Authors: Venjakob Christine , Leonhardt Sara , Klein Alexandra-Maria
Published: January 2020

Nectar is crucial to maintain plant-pollinator mutualism. Nectar quality (nutritional composition) can vary strongly between individuals of the same plant species. The factors driving such inter-individual variation have however not been investigated closer. We investigated nectar quality of field scabious, Knautia arvensis in different grassland plant communities varying in species composition and richness to assess whether nectar quality can be affected by the surrounding plant community. We analyzed (with high performance liquid chromatography) the content of carbohydrates, overall amino acids, and essential amino acids. Amino acid and carbohydrate concentrations and proportions varied among plant individuals and with the surrounding plant community but were not related to the surrounding plant species richness. Total and individual carbohydrate concentrations were lowest, while proportions of the essential amino acids, valine, isoleucine, leucine (all phagostimulatory), and lysine were highest in plant species communities of the highest diversity. Our results show that K. arvensis nectar chemistry varies with the composition of the surrounding plant community, which may alter the taste and nutritional value and thus affect the plant’s visitor spectrum and visitation rate. However, the strong inter-individual variation in nectar quality requires additional studies (e.g., in semi-field studies) to disentangle different biotic and abiotic factors contributing to inter-individual nectar chemistry in a plant-community context.
Larval Host Preference and Suitability for the Sawfly Mesoneura rufonota among Five Cinnamomun Tree Species
Authors: Zhou Jiaying , Zhang Jiangtao , Tregenza Tom , Pan Youliang , Wang Qinzhao , Shi Haoni , Liu Xingping
Published: January 2020

The camphor sawfly, Mesoneura rufonota Rohwer, is an economically important leaf-chewing pest of the genus Cinnamomum Trew. However, little is known about the range of species that it can infest within this genus or whether larvae show preferences for particular species. We conducted preference and performance experiments under laboratory conditions to assess larval host preference and suitability as a host plant of five congeneric trees species: C. camphora (Linn) Presl, C. bodinieri Levl., C. burmanni (Nees et T. Nees) Blume, C. pauciflorum Nees, and C. micranthum (Hay.) Hay. In no-choice, two-choice and multiple-choice feeding trials, significantly higher feeding rates were found on C. camphora, followed by C. bodinieri, compared to those on the other three tree species. In two-choice behavior trials, larvae moved to occupy leaves of C. camphora faster and more frequently, followed by C. bodinieri, than when offered the other three tree species. In no-choice fitness trials, the survival of larval and pupal stage was the highest, the developmental duration of larval and pupal stage was the shortest, the pupal body weight was the heaviest and adults lived the longest on C. camphora followed by C. bodinieri, while M. rufonota did not complete development on C. burmanni, C. pauciflorum or C. micranthum. Our results demonstrate that larvae consistently prefer and perform better on C. camphora leaves, that they can utilize C. bodinieri, but it is less preferred, and that C. burmanni, C. pauciflorum, and C. micranthum appear to be unsuitable as a host for M. rufonota.
Novel Metformin-Based Schiff Bases: Synthesis, Characterization, and Antibacterial Evaluation
Authors: Al-Qadsy Inas , Saeed Waseem Sharaf , Al-Odayni Abdel-Basit , Ahmed Saleh Al-Faqeeh Lena , Alghamdi Abdulaziz Ali , Farooqui Mazahar
Published: January 2020

Novel Schiff bases of metformin hydrochloride and (ortho)para-nitrobenzaldehyde were synthesized by employing two efficient environmentally friendly methods, namely, stirring and microwave-assisted methods using water as the solvent. The advantage of microwave irradiation over the other methods was represented in the reduction of reaction time and wastes, and good yields; however, water in both methods plays the role of eco-friendly solvent. The structural properties of the (ortho)para-isomer products were analyzed by elemental analysis, Fourier transform infrared (FTIR) spectroscopy, UV-Visible (UV-Vis) spectroscopy, 1H nuclear magnetic resonance (NMR) spectroscopy, 13C NMR spectroscopy, mass spectroscopy, and differential scanning calorimetry (DSC). The newly synthesized compounds were screened for their antibacterial activity against selected Gram-positive (ATCC 25923, ATCC 43300, and ATCC 29212) and Gram-negative (ATCC 25922, ATCC 27853, and ATCC 700603) bacteria using the agar well diffusion method. Compared with the standard drug streptomycin, both Schiff bases exhibited moderate bactericidal activity against the tested bacteria with higher values of ortho-nitro compared with the para-nitro isomer; however, no effect on ATCC 43300 and ATCC 27853 was observed under the experimental conditions employed.