bluuebunny/crossref_metadata_2025 huggingface dataset:
Quasicrystal Tilings in Three Dimensions and Their Empires
Authors: Hammock Dugan , Fang Fang , Irwin Klee
Published: September 2018

The projection method for constructing quasiperiodic tilings from a higher dimensional lattice provides a useful context for computing a quasicrystal’s vertex configurations, frequencies, and empires (forced tiles). We review the projection method within the framework of the dual relationship between the Delaunay and Voronoi cell complexes of the lattice being projected. We describe a new method for calculating empires (forced tiles) which also borrows from the dualisation formalism and which generalizes to tilings generated projections of non-cubic lattices. These techniques were used to compute the vertex configurations, frequencies and empires of icosahedral quasicrystals obtained as a projections of the D 6 and Z 6 lattices to R 3 and we present our analyses. We discuss the implications of this new generalization.
Liquid Biopsy Potential Biomarkers in Prostate Cancer
Authors: Neuhaus Jochen , Yang Bo
Published: September 2018

Prostate cancer (PCa) is the second most common cancer in men worldwide with an incidence of 14.8% and a mortality of 6.6%. Shortcomings in comprehensive medical check-ups in low- and middle-income countries lead to delayed detection of PCa and are causative of high numbers of advanced PCa cases at first diagnosis. The performance of available biomarkers is still insufficient and limited applicability, including logistical and financial burdens, impedes comprehensive implementation into health care systems. There is broad agreement on the need of new biomarkers to improve (i) early detection of PCa, (ii) risk stratification, (iii) prognosis, and (iv) treatment monitoring. This review focuses on liquid biopsy tests distinguishing high-grade significant (Gleason score (GS) ≥ 7) from low-grade indolent PCa. Available biomarkers still lack performance in risk stratification of biopsy naïve patients. However, biomarkers with highly negative predictive values may help to reduce unnecessary biopsies. Risk calculators using integrative scoring systems clearly improve decision-making for invasive prostate biopsy. Emerging biomarkers have the potential to substitute PSA and improve the overall performance of risk calculators. Until then, PSA should be used and may be replaced whenever enough evidence has accumulated for better performance of a new biomarker.
Inter-Worldview Education and the Re-Production of Good Religion
Authors: Moyaert Marianne
Published: September 2018

In this article, I focus on the increasing interest taken by European political and educational policy makers in inter-worldview education. My article has two parts. The first part consists of a document analysis of pivotal European publications on this and related issues. In the second and more critical part of this article, I make explicit my concerns about these European pleas for inter-worldview education. The main criticism that I present below is that the European policy on inter-worldview dialogue views the problem of intolerance too much as an individual problem that can and must be dealt with pedagogically, without recognizing that intolerance is just as much a structural socio-political problem. Important European policy documents, to be examined in the first part of this article, do not discuss how the way our societies are structured results in a sustenance of inequalities and in the marginalization of certain groups of people. At no point does these documents link inter-worldview education to the need “to examine the ideologies and structures of society critically,” as a consequence the plea for dialogue loses some of its critical and transforming potential.
A Distributed Strategy for Target Tracking and Rendezvous Using UAVs Relying on Visual Information Only
Authors: Andreetto Marco , Pacher Matteo , Macii David , Palopoli Luigi , Fontanelli Daniele
Published: September 2018

This paper proposes a distributed target tracking solution using a team of Unmanned Aerial Vehicles (UAVs) equipped with low-cost visual sensors capable of measuring targets bearing information only. The team of UAVs moves along circular orbits and uses consensus–based distributed Kalman Filtering to identify the position of the target. We show that the centre of the orbit eventually converges to the target position using theoretical arguments and extensive simulation data. By using the same approach, we can solve the rendezvous problem: the team first scans an area in search of a target; as soon as one of the UAVs spots, the other components converge on the target position.
Organizational and Systemic Policy Capacity of Government Organizations Involved in Energy-From-Waste (EFW) Development in Thailand
Authors: Chenboonthai Haruthai , Watanabe Tsunemi
Published: September 2018

This article studies the challenges of Thai energy-from-waste (EFW) development from an institutional perspective. Policy capacity, described as conditions for effective policy development and implementation, of the main government organizations involved in EFW development under the Alternative Energy Development Plan (AEDP) 2015 is examined. Adopting the variables used under the institutional analytical and development (IAD) approach, we modified the analytical framework for policy capacity by categorizing factors contributing to policy capacity into elements (skills, resources, and process) that affect the decisions and actions of actors of government organizations. Then, the results from the in-depth interview were interpreted through a modified analytical framework to examine policy capacity at the organizational and systemic level of government organizations involved in EFW development. We believe that a modified analytical framework for policy capacity is compatible with the IAD approach and can facilitate the utilization of policy capacity for further analysis under the IAD approach. Moreover, the modified framework can encourage a better understanding of current policy capacity and its impacts on other organizations, since an organization values its own policy capacity and others’ policy capacity differently. Consequently, this understanding can benefit the improvement of cooperation among Thai government organizations involved in EFW development.
Proactive Caching at the Edge Leveraging Influential User Detection in Cellular D2D Networks
Authors: Said Anwar , Shah Syed Waqas Haider , Farooq Hasan , Mian Adnan Noor , Imran Ali , Crowcroft Jon
Published: September 2018

Caching close to users in a radio access network (RAN) has been identified as a promising method to reduce a backhaul traffic load and minimize latency in 5G and beyond. In this paper, we investigate a novel community detection inspired by a proactive caching scheme for device-to-device (D2D) enabled networks. The proposed scheme builds on the idea that content generated/accessed by influential users is more probable to become popular and thus can be exploited for pro-caching. We use a Clustering Coefficient based Genetic Algorithm (CC-GA) for community detection to discover a group of cellular users present in close vicinity. We then use an Eigenvector Centrality measure to identify the influential users with respect to the community structure, and the content associated to it is then used for pro-active caching using D2D communications. The numerical results show that, compared to reactive caching, where historically popular content is cached, depending on cache size, load and number of requests, up to 30% more users can be satisfied using a proposed scheme while achieving significant reduction in backhaul traffic load.
The Formation of Fullerenes in Planetary Nebulae
Authors: Cami Jan , Peeters Els , Bernard-Salas Jeronimo , Doppmann Greg , De Buizer James
Published: September 2018

In the last decade, fullerenes have been detected in a variety of astrophysical environments, with the majority being found in planetary nebulae. Laboratory experiments have provided us with insights into the conditions and pathways that can lead to fullerene formation, but it is not clear precisely what led to the formation of astrophysical fullerenes in planetary nebulae. We review some of the available evidence, and propose a mechanism where fullerene formation in planetary nebulae is the result of a two-step process where carbonaceous dust is first formed under unusual conditions; then, the fullerenes form when this dust is being destroyed.
B Chromosomes of the Asian Seabass (Lates calcarifer) Contribute to Genome Variations at the Level of Individuals and Populations
Authors: Komissarov Aleksey , Vij Shubha , Yurchenko Andrey , Trifonov Vladimir , Thevasagayam Natascha , Saju Jolly , Sridatta Prakki Sai Rama , Purushothaman Kathiresan , Graphodatsky Alexander , Orbán László , Kuznetsova Inna
Published: September 2018

The Asian seabass (Lates calcarifer) is a bony fish from the Latidae family, which is widely distributed in the tropical Indo-West Pacific region. The karyotype of the Asian seabass contains 24 pairs of A chromosomes and a variable number of AT- and GC-rich B chromosomes (Bchrs or Bs). Dot-like shaped and nucleolus-associated AT-rich Bs were microdissected and sequenced earlier. Here we analyzed DNA fragments from Bs to determine their repeat and gene contents using the Asian seabass genome as a reference. Fragments of 75 genes, including an 18S rRNA gene, were found in the Bs; repeats represented 2% of the Bchr assembly. The 18S rDNA of the standard genome and Bs were similar and enriched with fragments of transposable elements. A higher nuclei DNA content in the male gonad and somatic tissue, compared to the female gonad, was demonstrated by flow cytometry. This variation in DNA content could be associated with the intra-individual variation in the number of Bs. A comparison between the copy number variation among the B-related fragments from whole genome resequencing data of Asian seabass individuals identified similar profiles between those from the South-East Asian/Philippines and Indian region but not the Australian ones. Our results suggest that Bs might cause variations in the genome among the individuals and populations of Asian seabass. A personalized copy number approach for segmental duplication detection offers a suitable tool for population-level analysis across specimens with low coverage genome sequencing.
Molecular Genotyping (SSR) and Agronomic Phenotyping for Utilization of Durum Wheat (Triticum durum Desf.) Ex Situ Collection from Southern Italy: A Combined Approach Including Pedigreed Varieties
Authors: Marzario Stefania , Logozzo Giuseppina , David Jacques L. , Zeuli Pierluigi Spagnoletti , Gioia Tania
Published: September 2018

In South Italy durum wheat (Triticum durum Desf.) has a long-time tradition of growing and breeding. Accessions collected and now preserved ex situ are a valuable genetic resource, but their effective use in agriculture and breeding programs remains very low. In this study, a small number (44) of simple sequence repeats (SSR) molecular markers were used to detect pattern of diversity for 136 accessions collected in South Italy over time, to identify the genepool of origin, and establish similarities with 28 Italian varieties with known pedigree grown in Italy over the same time-period. Phenotyping was conducted for 12 morphophysiological characters of agronomic interest. Based on discriminant analysis of principal components (DAPC) and STRUCTURE analysis six groups were identified, the assignment of varieties reflected the genetic basis and breeding strategies involved in their development. Some “old” varieties grown today are the result of evolution through natural hybridization and conservative pure line selection. A small number of molecular markers and little phenotyping coupled with powerful statistical analysis and comparison to pedigreed varieties can provide enough information on the genetic structure of durum wheat germplasm for a quick screening of the germplasm collection able to identify accessions for breeding or introduction in low input agriculture.
Suitability of Boulder Marl and Marsh Clay as Sealing Substrates for Landfill Capping Systems—A Practical Comparison
Authors: Beck-Broichsitter Steffen , Gerke Horst H. , Horn Rainer
Published: September 2018

The effects of compaction on soil shrinkage behavior need to be considered for engineering long-term durable mineral liners of landfill capping systems. For this purpose, a new three-dimensional laser scanning device was coupled with a mathematical-empirical model to simultaneously determine the shrinkage behavior of a boulder marl (bm) and a marsh clay (mc). Therefore, both materials were precompacted in 200 soil cores (100 cm3) on the basis of the Proctor test results with five different degrees of compaction (bm1-bm5; mc1-mc5). Thus, the shrinkage behavior, intensity, and tendency were determined during a standardized drying experiment. The volume shrinkage index was used to describe the pore size dependent shrinkage tendency and was classified as high to very high (11.3–17.7%) for the marsh clay and medium (5.3–9.2%) for the boulder marl. Additionally, only the boulder marl (bm2), compacted up to 88% of Proctor density, could be installed as landfill bottom liner in drier locations if the local matric potentials did not exceed the previously highest observed drying range (i.e. values below −300 hPa), to avoid crack formation and generation.
Using a Multi-Proxy Approach to Detect and Date a Buried part of the Hellenistic City Wall of Ainos (NW Turkey)
Authors: Seeliger Martin , Pint Anna , Frenzel Peter , Weisenseel Paula , Erkul Ercan , Wilken Dennis , Wunderlich Tina , Başaran Sait , Bücherl Heike , Herbrecht Marina , Rabbel Wolfgang , Schmidts Thomas , Szemkus Nina , Brückner Helmut
Published: September 2018

Throughout mankind’s history, the need to secure and protect the home settlement was an essential one. This holds especially true for the city of Ainos (modern Enez) in Turkish Thrace. Due to its continuous settlement history since the 7th/6th century BC, several different types of city walls were built—sometimes even on top of each other—several of which have been preserved over time. To decipher the construction style, the course and the age of a buried city wall segment in the southern part of the former city, a geoscientific multi-proxy approach including magnetic gradiometer (MG) and electrical resistivity tomography (ERT) measurements in combination with granulometrical, sedimentological and microfaunistical investigations on sediment cores was applied. We were able to (1) present reasonable arguments for its Hellenistic age; (2) reveal the course of this wall segment and extrapolate it further north into a less studied area; and (3) demonstrate that in this near-coastal area, the former swampy terrain had been consolidated for constructing the wall. Our multi-proxy approach serves as a valuable example for investigating buried structures in archaeological contexts, avoiding a less-economical, time-consuming, or even forbidden excavation.
Prediction of Holocene Mercury Accumulation Trends by Combining Palynological and Geochemical Records of Lake Sediments (Black Forest, Germany)
Authors: Schütze Martin , Tserendorj Gegeensuvd , Pérez-Rodríguez Marta , Rösch Manfred , Biester Harald
Published: September 2018

Forest vegetation plays a key role in the cycling of mercury (Hg) and organic matter (OM) in terrestrial ecosystems. Litterfall has been indicated as the major transport vector of atmospheric Hg to forest soils, which is eventually transported and stored in the sediments of forest lakes. Hence, it is important to understand how changes in forest vegetation affect Hg in soil and its biogeochemical cycling in lake systems. We investigated the pollen records and the geochemical compositions of sediments from two lakes (Schurmsee and Glaswaldsee) in the Black Forest (Germany) to evaluate whether long-term shifts in forest vegetation induced by climate or land use influenced Hg accumulation in the lakes. We were particularly interested to determine whether coniferous forests were associated with a larger export of Hg to aquatic systems than deciduous forests. Principal components analysis followed by principal component regression enabled us to describe the evolution of the weight of the latent processes determining the accumulation of Hg over time. Our results emphasize that the in-lake uptake of Hg during warm climate periods, soil erosion after deforestation and emissions from mining and other human activities triggered changes in Hg accumulation during the Holocene stronger than the changes caused by forest vegetation alone.
Nordic Modernism for Beginners
Authors: Brantly Susan C.
Published: September 2018

This essay proposes a narrative of the Nordic countries’ relationship to modernism and other major literary trends of the late 19th and 20th centuries, that situates them in conjunction with the rest of Europe. “Masterpieces of Scandinavian Literature: the 20th Century” is a course that has been taught to American college students without expertise in literature or Scandinavia for three decades. This article describes the content and methodologies of the course and how Nordic modernisms are explained to this particular audience of beginners. Simple definitions of modernism and other related literary movements are provided. By focusing on this unified literary historical narrative and highlighting the pioneers of Scandinavian literature, the Nordic countries are presented as solid participants in European literary and cultural history. Further, the social realism of the Modern Breakthrough emerges as one of the Nordic countries distinct contributions to world literature.
Images of the Crowned Buddha along the Silk Road: Iconography and Ideology
Authors: Twist Rebecca L.
Published: September 2018

The interpretation of early Buddha images with a crown has long been a source of debate. Many scholars have concluded that the iconography of the crown is intended to denote Śākyamuni as a cakravartin or universal Buddha. A few have suggested it represents a sambhogakāya Buddha in Mahāyāna Buddhism. This art historical and Buddhological study examines the visual record of early crowned Buddhas along the Silk Road, focusing on the iconographic signifiers of the crown, silk items, and ornaments, and interprets them within a broader framework of Buddhist theoretical principals and practice. Not only is this a visual analysis of iconography, it also considers contemporary Buddhist literary evidence that shows the development of the iconography and ideology of the crowned Buddha. As a result of this examination, I propose that the recurring iconographic evidence and the textual evidence underscore the intention to depict a form of sambhogakāya Buddha as an early esoteric meditational construct. Moreover, many Buddhas perform one of the two mudrās that are particular to the esoteric form of Vairocana Buddha. Therefore, the iconography also signifies the ideology of the archetypal Ādi Buddha as an esoteric conception.
Plant Production in Controlled Environments
Authors: Niu Genhua , Masabni Joseph
Published: September 2018

Crop production in open fields is increasingly limited by weather extremes and water shortages, in addition to pests and soil-borne diseases. In order to increase crop yield, quality, and productivity, controlled environment agriculture (CEA) can play an important role as an alternative and supplemental production system to conventional open field production. CEA is any agricultural technology that enables growers to manipulate the growing environment for improved yield and quality. CEA production systems include high tunnels, greenhouses, and indoor vertical farming, as well as hydroponics and aquaponics. Currently, ‘low-tech’ CEA techniques such as high tunnels (plastic greenhouses with minimum or no cooling and heating) are primarily utilized in developing countries where labor costs are relatively low, and China has by far the largest area covered by high tunnels or ‘Chinese-style’ solar greenhouses. The most control-intensive ‘high-tech’ CEA approach, namely indoor vertical farming, has gained tremendous attention in the past decade by researchers and entrepreneurs around the world, owing to advancements in lighting technology, including use of light emitting diodes (LEDs), and increasing urbanization with new market opportunities. This special issue covers some of the CEA topics such as LED lighting, substrate, and hydroponics.
Perceptions about the Federally Mandated Smoke-Free Housing Policy among Residents Living in Public Housing in New York City
Authors: Jiang Nan , Thorpe Lorna , Kaplan Sue , Shelley Donna
Published: September 2018

Background: To assess residents’ attitudes towards the United States (U.S.) Department of Housing and Urban Development’s new smoke-free public housing policy, perceptions about barriers to policy implementation, and suggestions for optimizing implementation. Methods: In 2017, we conducted 10 focus groups among 91 residents (smokers and nonsmokers) living in New York City public housing. Results: Smokers and nonsmokers expressed skepticism about the public housing authority’s capacity to enforce the policy due to widespread violations of the current smoke-free policy in common areas and pervasive use of marijuana in buildings. Most believed that resident engagement in the roll-out and providing smoking cessation services was important for compliance. Resident expressed concerns about evictions and worried that other building priorities (i.e., repairs, drug use) would be ignored with the focus now on smoke-free housing. Conclusions: Resident-endorsed strategies to optimize implementation effectiveness include improving the access to cessation services, ongoing resident engagement, education and communication to address misconceptions and concerns about enforcement, and placing smoke-free homes in a larger public housing authority healthy housing agenda.
Effects of a 36-h Survival Training with Sleep Deprivation on Oxidative Stress and Muscle Damage Biomarkers in Young Healthy Men
Authors: Jówko Ewa , Różański Paweł , Tomczak Andrzej
Published: September 2018

The aim of this study was to analyze changes in oxidative stress and muscle damage markers during a 36-h survival training combined with sleep deprivation. The study included 23 male students of physical education (specialty: Physical Education for Uniformed Services), randomly divided into the survival or control group. The students in the survival group completed a 36-h survival training with moderate to low physical activity, without the possibility to sleep. The students in the control group performed only physical activity included in daily routines and had a normal sleep pattern. No significant changes in measured parameters were seen in the control group throughout the study period. In the survival group, plasma lipid hydroperoxides (LHs) and creatine kinase (CK) activity increased at 24 h and remained elevated up to 36 h (main effects for LHs: time, p = 0.006 and group × time, p = 0.00008; main effects for CK: time, p = 0.000001, group, p = 0.005, and group × time, p = 0.000001). A 12-h recovery was sufficient to normalize both LHs and CK to the pre-training level; in fact, the post-recovery LHs and CK levels were even lower than at baseline. Residual total antioxidant capacity (TAC) of plasma (without the major constituents: uric acid and albumin) was elevated at both 24 h and 36 h of survival training, but not following a 12-h recovery (main effects: group, p = 0.001 and group × time, p = 0.04). In turn, the activity of glutathione peroxidase (GPx) in whole blood and superoxide dismutase (SOD) in erythrocytes decreased between 24 h and 36 h of survival training (main group effect for GPx, p = 0.038 and SOD, p = 0.045). In conclusion, these findings imply that a 36-h survival training with sleep deprivation impairs enzymatic antioxidant defense, increases lipid peroxidation, and induces muscle damage. Our findings also indicate that at least in the case of young physically active men, a 12-h recovery after the 36-h period of physical activity with sleep deprivation may be sufficient for the normalization of oxidative and muscle damage markers and restoration of blood prooxidant-antioxidant homeostasis.
Family Patterns and Suicidal and Violent Behavior among Adolescent Girls—Genogram Analysis
Authors: Sitnik-Warchulska Katarzyna , Izydorczyk Bernadetta
Published: September 2018

An increase in extreme self-destructive and aggressive behaviors in adolescents has been observed in recent years. Therapeutic effectiveness seems to depend on an adequate recognition of factors that might increase the risk of extreme destructive behaviors. The aim of this study was to identify the family patterns that should draw therapeutic attention due to their importance for suicidal or violent behaviors in adolescent girls. The study involved 140 participants, aged 13–17, including 40 girls after suicide attempts, 40 girls using violence, and 60 girls without destructive behavior at all. The Genograms were used to assess the family structure, relationships between family members, and family projections. The data was analyzed by polynomial logistic regression, analysis of variance, and discriminant analysis. Emotional distance and hidden projections (related to diseases) were the most significant predictors of suicidal behaviors in the studied adolescent girls. Alcohol abuse by significant family members, especially by male family members, and a lesser role of hidden projections, were the most significant predictors of violent behaviors. Interventions designed to reduce risk of destructive behaviors among adolescents need to take account of the specific family patterns.
Perfluorooctanoic Acid (PFOA) Exposure in Early Life Increases Risk of Childhood Adiposity: A Meta-Analysis of Prospective Cohort Studies
Authors: Liu Pingping , Yang Fang , Wang Yongbo , Yuan Zhanpeng
Published: September 2018

Some articles have examined perfluorooctanoic acid (PFOA) exposure in early life in relation to risk of childhood adiposity. Nevertheless, the results from epidemiological studies exploring the associations remain inconsistent and contradictory. We thus conducted an analysis of data currently available to examine the association between PFOA exposure in early life and risk of childhood adiposity. The PubMed, EMBASE, and Web of Science databases were searched to identify studies that examined the impact of PFOA exposure in early life on childhood adiposity. A random-effects meta-analysis model was used to pool the statistical estimates. We identified ten prospective cohort studies comprising 6076 participants with PFOA exposure. The overall effect size (relative risk or odds ratio) for childhood overweight was 1.25 (95% confidence interval (CI): 1.04, 1.50; I2 = 40.5%). In addition, exposure to PFOA in early life increased the z-score of childhood body mass index (β = 0.10, 95% CI: 0.03, 0.17; I2 = 27.9%). Accordingly, exposure to PFOA in early life is associated with an increased risk for childhood adiposity. Further research is needed to verify these findings and to shed light on the molecular mechanism of PFOA in adiposity.
Associations Between the Dopamine D4 Receptor and DAT1 Dopamine Transporter Genes Polymorphisms and Personality Traits in Addicted Patients
Authors: Chmielowiec Jolanta , Chmielowiec Krzysztof , Suchanecka Aleksandra , Trybek Grzegorz , Mroczek Bożena , Małecka Iwona , Grzywacz Anna
Published: September 2018

Many factors are involved in addiction. The dopaminergic system is thought to be the key element in this process. The mesolimbic dopamine system is a crucial element in the reward system. Changes in this system are thought to be leading to substance use disorders and dependence. Therefore, for our study we chose an analysis of two polymorphisms in genes (Variable Number of Tandem Repeats in DRD4 and DAT1) responsible for dopaminergic transmission, which might be implicated in the scores of personality traits measured by the NEO-FFI test. The study group consisted of 600 male volunteers—299 addicted subjects and 301 controls. Both groups were recruited by psychiatrists; in the case group addiction was diagnosed; in the controls a mental illness was excluded. In both groups the same psychometric test and genotyping by the PCR VNTR method were performed. The results were investigated by a multivariate analysis of the main effects ANOVA. In the presented study no DRD4 main effects were found for any of the analyzed traits but the DRD4 main effects approximated to the statistical significance for the extraversion scale. However, no DAT1 main effects were found for any of the analyzed traits but the DAT1 main effects approximated to the statistical significance for the agreeability scale.These associations open new possibilities for addiction research.
Erratum: Assari, S.; Mistry, R. Educational Attainment and Smoking Status in a National Sample of American Adults; Evidence for the Blacks’ Diminished Return. Int. J. Environ. Res. Public Health 2018, 15, 763
Authors: Assari Shervin , Mistry Ritesh
Published: September 2018

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Global Signaling Profiling in a Human Model of Tumorigenic Progression Indicates a Role for Alternative RNA Splicing in Cellular Reprogramming
Authors: Caruso Joseph A. , Carruthers Nicholas J. , Thibodeau Bryan , Geddes Timothy J. , Dombkowski Alan A. , Stemmer Paul M.
Published: September 2018

Intracellular signaling is controlled to a large extent by the phosphorylation status of proteins. To determine how human breast cells can be reprogrammed during tumorigenic progression, we profiled cell lines in the MCF10A lineage by phosphoproteomic analyses. A large cluster of proteins involved in RNA splicing were hypophosphorylated as cells progressed to a hyperplastic state, and then hyperphosphorylated after progression to a fully metastatic phenotype. A comprehensive transcriptomic approach was used to determine whether alterations in splicing factor phosphorylation status would be reflected in changes in mRNA splicing. Results indicated that the degree of mRNA splicing trended with the degree of tumorigenicity of the 4 cell lines tested. That is, highly metastatic cell cultures had the greatest number of genes with splice variants, and these genes had greater fluctuations in expression intensities. Genes with high splicing indices were mapped against gene ontology terms to determine whether they have known roles in cancer. This group showed highly significant associations for angiogenesis, cytokine-mediated signaling, cell migration, programmed cell death and epithelial cell differentiation. In summary, data from global profiling of a human model of breast cancer development suggest that therapeutics should be developed which target signaling pathways that regulate RNA splicing.
ZKSCAN3 Upregulation and Its Poor Clinical Outcome in Uterine Cervical Cancer
Authors: Lee Sun , Cho Young-Eun , Kim Joo-Young , Park Jae-Hoon
Published: September 2018

Zinc finger with KRAB and SCAN domain 3 (ZKSCAN3) upregulates genes encoding proteins involved in cell differentiation, proliferation and apoptosis. ZKSCAN3 has been reported to be overexpressed in several human cancers such as colorectal cancer and prostate cancer and is proposed as a candidate oncoprotein. However, the molecular mechanism by which ZKSCAN3 participates in carcinogenesis is largely unknown. Here, we evaluated ZKSCAN3 expression in uterine cervical cancers (CC) by immunohistochemistry using formalin-fixed, paraffin-embedded tissues from 126 biopsy samples from 126 patients. The clinicopathological findings were analyzed and compared with ZKSCAN3 expression levels. ZKSCAN3 was strongly overexpressed in CCs compared to adjacent non-neoplastic cervical mucosa tissues. Moreover, a gene copy number assay showed amplified ZKSCAN3 in CC samples. ZKSCAN3 overexpression was also significantly associated with poor overall survival of the patients. Overall, our findings indicate that ZKSCAN3 overexpression is a frequent event in uterine CC and is correlated with a poor clinical outcome. ZKSCAN3 could be developed as a molecular marker for prognostic prediction and early detection.
Thioredoxin Confers Intrinsic Resistance to Cytostatic Drugs in Human Glioma Cells
Authors: Haas Bodo , Schütte Lena , Wos-Maganga Maria , Weickhardt Sandra , Timmer Marco , Eckstein Niels
Published: September 2018

Thioredoxin (Trx) overexpression is known to be a cause of chemotherapy resistance in various tumor entities. However, Trx effects on resistance are complex and depend strictly on tissue type. In the present study, we analyzed the impact of the Trx system on intrinsic chemoresistance of human glioblastoma multiforme (GBM) cells to cytostatic drugs. Resistance of GBM cell lines and primary cells to drugs and signaling inhibitors was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays. Impact of Trx inhibition on apoptosis was investigated by proteome profiling of a subset of proteins and annexin V apoptosis assays. Trx-interacting protein (TXNIP) was overexpressed by transfection and protein expression was determined by immunoblotting. Pharmacological inhibition of Trx by 1-methyl-2-imidazolyl-disulfide (PX-12) reduced viability of three GBM cell lines, induced expression of active caspase-3, and reduced phosphorylation of AKT-kinase and expression of β-catenin. Sensitivity to cisplatin could be restored by both PX-12 and recombinant expression of the upstream Trx inhibitor TXNIP, respectively. In addition, PX-12 also sensitized primary human GBM cells to temozolomide. Combined inhibition of Trx and the phosphatidylinositide 3-kinase (PI3K) pathway resulted in massive cell death. We conclude that the Trx system and the PI3K pathway act as a sequential cascade and could potentially present a new drug target.
Blood-Based Cancer Biomarkers in Liquid Biopsy: A Promising Non-Invasive Alternative to Tissue Biopsy
Authors: Marrugo-Ramírez José , Mir Mònica , Samitier Josep
Published: September 2018

Cancer is one of the greatest threats facing our society, being the second leading cause of death globally. Currents strategies for cancer diagnosis consist of the extraction of a solid tissue from the affected area. This sample enables the study of specific biomarkers and the genetic nature of the tumor. However, the tissue extraction is risky and painful for the patient and in some cases is unavailable in inaccessible tumors. Moreover, a solid biopsy is expensive and time consuming and cannot be applied repeatedly. New alternatives that overcome these drawbacks are rising up nowadays, such as liquid biopsy. A liquid biopsy is the analysis of biomarkers in a non-solid biological tissue, mainly blood, which has remarkable advantages over the traditional method; it has no risk, it is non-invasive and painless, it does not require surgery and reduces cost and diagnosis time. The most studied cancer non-invasive biomarkers are circulating tumor cells (CTCs), circulating tumor DNA (ctDNA), and exosomes. These circulating biomarkers play a key role in the understanding of metastasis and tumorigenesis, which could provide a better insight into the evolution of the tumor dynamics during treatment and disease progression. Improvements in isolation technologies, based on a higher grade of purification of CTCs, exosomes, and ctDNA, will provide a better characterization of biomarkers and give rise to a wide range of clinical applications, such as early detection of diseases, and the prediction of treatment responses due to the discovery of personalized tumor-related biomarkers.
Emerging Roles of Cardiotrophin-1 in the Pathogenesis and Biomarker of Atherosclerosis
Authors: Watanabe Takuya , Konii Hanae , Sato Kengo
Published: September 2018

Cardiotrophin-1 (CT-1), an interleukin-6 family cytokine, is known as an active inducer capable of cardiac hypertrophy and vascular stiffness in hypertensive heart disease. CT-1 is expressed at high levels in the heart, vascular endothelial cells (ECs), and adipocytes. CT-1 stimulates inflammatory and proatherogenic molecule expression in human monocytes and ECs, as well as monocyte-EC adhesion. CT-1 enhances oxidized low-density lipoprotein-induced foam-cell formation in human monocyte-derived macrophages. CT-1 stimulates the migration, proliferation, and colloagen-1 production in human vascular smooth muscle cells. Chronic CT-1 infusion into Apoe−/− mice accelerates the development of aortic atherosclerotic lesions. CT-1 is expressed at high levels in ECs and macrophage foam cells within atheromatous plaques in Apoe−/− mice. A blockade of CT-1 using anti-CT-1 neutralizing antibody results in the prevention of atherogenesis in Apoe−/− mice. Plasma CT-1 concentrations are elevated in patients with hypertensive heart disease, ischemic heart disease, and metabolic syndrome, and are positively associated with the severity of cardiac hypertrophy, heart failure, and atherosclerosis. Increased plasma concentration of CT-1 is a predictor of death and heart failure following acute myocardial infarction. Therefore, CT-1 serves a novel therapeutic target for atherosclerosis and related diseases. Plasma CT-1 may be a reliable biomarker for atherosclerotic cardiovascular diseases.
Effects of Progressive Resistance Training on Post-Surgery Incontinence in Men with Prostate Cancer
Authors: Park Juhyun , Yoon Dong , Yoo Sangjun , Cho Sung , Cho Min , Han Ga-Young , Song Wook , Jeong Hyeon
Published: September 2018

We evaluated the efficacy of progressive resistance training of the pelvic floor muscle for post-prostatectomy incontinence. In this prospective study, 59 patients who underwent radical prostatectomy were evaluated preoperatively. Continence was sequentially assessed within 2 weeks postoperatively, and an exercise regimen was initiated at 6- and 12-weeks. The primary outcome was continent status and the secondary outcome was changes in muscle strength and endurance after the exercise intervention. Continence was defined as no urine loss in a 1h pad test. A total of 59 patients participated in this study. Six patients dropped out of the study because of non-compliance and orthopedic problems. Of the remaining 53 patients, 31 (58.5%) achieved pad-free continence at 12 weeks postoperatively. The patients were divided into two groups based on their continence status, and no statistically significant difference was observed in age, body mass index, prostate volume, prostate-specific antigen, pathological Gleason score sum, and pathological T stage. Meanwhile, preoperative maximal urethral closure pressure and change in hip extensor muscle strength and endurance during the 12-week exercise program were significantly higher in the continent group. In multivariate analysis, change in hip extensor muscle strength was the only significant parameter predicting achievement of continence status (Odds ratio, 1.039; p = 0.045). The changes in hip extensor muscle strength in the current exercise program was an independent predictor of continence status after radical prostatectomy. A large-scale prospective study on the relationship between extensor muscle strength and urinary incontinence should be explored in future.
Dehydroepiandrosterone Ameliorates Abnormal Mitochondrial Dynamics and Mitophagy of Cumulus Cells in Poor Ovarian Responders
Authors: Li Chia-Jung , Chen San-Nung , Lin Li-Te , Chern Chyi-Uei , Wang Peng-Hui , Wen Zhi-Hong , Tsui Kuan-Hao
Published: September 2018

Mitochondrial dysfunction is related to reproductive decline in humans, with consequences for in vitro fertilization (IVF). We assessed whether dehydroepiandrosterone (DHEA) could regulate mitochondrial homeostasis and mitophagy of cumulus cells (CCs) in poor ovarian responders (PORs). A total of 66 women who underwent IVF treatment at the Reproductive Medicine Center of Kaohsiung Veterans General Hospital were included in this study. Twenty-eight normal ovarian responders (NOR) and 38 PORs were enrolled. PORs were assigned to receive DHEA supplementation (n = 19) or not (n = 19) before IVF cycles. DHEA prevents mitochondrial dysfunction by decreasing the activation of DNM1L and MFF, and increasing MFN1 expression. Downregulation of PINK1 and PRKN occurred after DHEA treatment, along with increased lysosome formation. DHEA not only promoted mitochondrial mass but also improved mitochondrial homeostasis and dynamics in the CCs of POR. We also observed effects of alterations in mRNAs known to regulate mitochondrial dynamics and mitophagy in the CCs of POR. DHEA may prevent mitochondrial dysfunction through regulating mitochondrial homeostasis and mitophagy.
Phenomic Impact of Genetically-Determined Euthyroid Function and Molecular Differences between Thyroid Disorders
Authors: Ravera Silvia , Carrasco Nancy , Gelernter Joel , Polimanti Renato
Published: September 2018

Background: The thyroid plays a key role in development and homeostasis, but it has been difficult to establish causality with diseases and phenotypic traits because of several potential confounders. Methods: To determine the causal effect of euthyroid function, we conducted a two-sample Mendelian randomization study of euthyroid thyrotropin (TSH) and free thyroxine (FT4) levels with respect to 2419 traits assessed in 337,199 individuals from UK Biobank. Additionally, we investigated the molecular differences between hypothyroidism and hyperthyroidism using genome-wide data. Results: After multiple testing correction, sixteen traits appear to be affected by genetically-determined euthyroid TSH, including multiple thyroid-related traits, e.g., hypothyroidism (p = 2.39 × 10−17), height (p = 2.76 × 10−10), body fat distribution (impedance of whole body, p = 4.43 × 10−8), pulse rate (p = 2.84 × 10−8), female infertility (p = 4.91 × 10−6), and hearing aid use (p = 7.10 × 10−5). Moreover, we found a consistent genetic correlation between hypothyroidism and hyperthyroidism (rg = 0.45, p = 5.45 × 10−6) with several immune pathways shared between these diseases. Two molecular pathways survived multiple testing correction for specificity to hyperthyroidism, JAK/STAT signaling (p = 1.02 × 10−6) and Rac guanyl-nucleotide exchange factor activity (p = 4.39 × 10−6). Conclusion: Our data shed new light on the inter-individual variability of euthyroid function and the molecular mechanisms of the two thyroid disorders investigated.
Hyperspectral Imaging Using Laser Excitation for Fast Raman and Fluorescence Hyperspectral Imaging for Sorting and Quality Control Applications
Authors: Gruber Florian , Wollmann Philipp , Grählert Wulf , Kaskel Stefan
Published: September 2018

A hyperspectral measurement system for the fast and large area measurement of Raman and fluorescence signals was developed, characterized and tested. This laser hyperspectral imaging system (Laser-HSI) can be used for sorting tasks and for continuous quality monitoring. The system uses a 532 nm Nd:YAG laser and a standard pushbroom HSI camera. Depending on the lens selected, it is possible to cover large areas (e.g., field of view (FOV) = 386 mm) or to achieve high spatial resolutions (e.g., 0.02 mm). The developed Laser-HSI was used for four exemplary experiments: (a) the measurement and classification of a mixture of sulphur and naphthalene; (b) the measurement of carotenoid distribution in a carrot slice; (c) the classification of black polymer particles; and, (d) the localization of impurities on a lead zirconate titanate (PZT) piezoelectric actuator. It could be shown that the measurement data obtained were in good agreement with reference measurements taken with a high-resolution Raman microscope. Furthermore, the suitability of the measurements for classification using machine learning algorithms was also demonstrated. The developed Laser-HSI could be used in the future for complex quality control or sorting tasks where conventional HSI systems fail.
Support Structures for Additive Manufacturing: A Review
Authors: Jiang Jingchao , Xu Xun , Stringer Jonathan
Published: September 2018

Additive manufacturing (AM) has developed rapidly since its inception in the 1980s. AM is perceived as an environmentally friendly and sustainable technology and has already gained a lot of attention globally. The potential freedom of design offered by AM is, however, often limited when printing complex geometries due to an inability to support the stresses inherent within the manufacturing process. Additional support structures are often needed, which leads to material, time and energy waste. Research in support structures is, therefore, of great importance for the future and further improvement of additive manufacturing. This paper aims to review the varied research that has been performed in the area of support structures. Fifty-seven publications regarding support structure optimization are selected and categorized into six groups for discussion. A framework is established in which future research into support structures can be pursued and standardized. By providing a comprehensive review and discussion on support structures, AM can be further improved and developed in terms of support waste in the future, thus, making AM a more sustainable technology.
Personalised Medicine: The Odyssey from Hope to Practice
Authors: Visvikis-Siest Sophie , Gorenjak Vesna , Stathopoulou Maria G.
Published: September 2018

In this endeavour, inspired by the Odyssey, we aim to embark with the reader on a journey on a ship from Troy to Ithaca, coursing through the history of the momentous events and achievements that paved the way for personalised medicine. We will set sail amidst important genetic discoveries, beginning with the discovery of the first human genome, and voyage through the projects that contributed to the progress of pharmacogenomic studies. Concurrently, we will propose methods to overcome the obstacles that are slowing the potential full implementation of accumulated knowledge into everyday practice. This journey aims to reflect on the frontiers of current genetic knowledge and the practical use of this knowledge in preventive, diagnostic and pharmacogenomic approaches to directly impact the socio-economic aspects of public health.
Development and Experimental Evaluation of a Low-Cost Cooperative UAV Localization Network Prototype
Authors: Goel Salil , Kealy Allison , Lohani Bharat
Published: September 2018

Precise localization is one of the key requirements in the deployment of UAVs (Unmanned Aerial Vehicles) for any application including precision mapping, surveillance, assisted navigation, search and rescue. The need for precise positioning is even more relevant with the increasing automation in UAVs and growing interest in commercial UAV applications such as transport and delivery. In the near future, the airspace is expected to be occupied with a large number of unmanned as well as manned aircraft, a majority of which are expected to be operating autonomously. This paper develops a new cooperative localization prototype that utilizes information sharing among UAVs and static anchor nodes for precise positioning of the UAVs. The UAVs are retrofitted with low-cost sensors including a camera, GPS receiver, UWB (Ultra Wide Band) radio and low-cost inertial sensors. The performance of the low-cost prototype is evaluated in real-world conditions in partially and obscured GNSS (Global Navigation Satellite Systems) environments. The performance is analyzed for both centralized and distributed cooperative network designs. It is demonstrated that the developed system is capable of achieving navigation grade (2–4 m) accuracy in partially GNSS denied environments, provided a consistent communication in the cooperative network is available. Furthermore, this paper provides experimental validation that information sharing is beneficial to improve positioning performance even in ideal GNSS environments. The experiments demonstrate that the major challenges for low-cost cooperative networks are consistent connectivity among UAV platforms and sensor synchronization.