bluuebunny/crossref_metadata_2025 huggingface dataset:
Reduced cue-induced reinstatement of cocaine-seeking behavior in Plcb1 +/− mice
Authors: Cabana-Domínguez Judit , Martín-García Elena , Gallego-Roman Ana , Maldonado Rafael , Fernàndez-Castillo Noèlia , Cormand Bru
Published: October 2021

Cocaine addiction causes serious health problems, and no effective treatment is available yet. We previously identified a genetic risk variant for cocaine addiction in the PLCB1 gene and found this gene upregulated in postmortem brains of cocaine abusers and in human dopaminergic neuron-like cells after an acute cocaine exposure. Here, we functionally tested the contribution of the PLCB1 gene to cocaine addictive properties using Plcb1+/− mice. First, we performed a general phenotypic characterization and found that Plcb1+/− mice showed normal behavior, although they had increased anxiety and impaired short-term memory. Subsequently, mice were trained for operant conditioning, self-administered cocaine for 10 days, and were tested for cocaine motivation. After extinction, we found a reduction in the cue-induced reinstatement of cocaine-seeking behavior in Plcb1+/− mice. After reinstatement, we identified transcriptomic alterations in the medial prefrontal cortex of Plcb1+/− mice, mostly related to pathways relevant to addiction like the dopaminergic synapse and long-term potentiation. To conclude, we found that heterozygous deletion of the Plcb1 gene decreases cue-induced reinstatement of cocaine-seeking, pointing at PLCB1 as a possible therapeutic target for preventing relapse and treating cocaine addiction.
Sex-specific effects of polygenic risk for schizophrenia on lifespan cognitive functioning in healthy individuals
Authors: Koch Elise , Nyberg Lars , Lundquist Anders , Pudas Sara , Adolfsson Rolf , Kauppi Karolina
Published: October 2021

﻿Polygenic risk for schizophrenia has been associated with lower cognitive ability and age-related cognitive change in healthy individuals. Despite well-established neuropsychological sex differences in schizophrenia patients, genetic studies on sex differences in schizophrenia in relation to cognitive phenotypes are scarce. Here, we investigated whether the effect of a polygenic risk score (PRS) for schizophrenia on childhood, midlife, and late-life cognitive function in healthy individuals is modified by sex, and if PRS is linked to accelerated cognitive decline. Using a longitudinal data set from healthy individuals aged 25–100 years (N = 1459) spanning a 25-year period, we found that PRS was associated with lower cognitive ability (episodic memory, semantic memory, visuospatial ability), but not with accelerated cognitive decline. A significant interaction effect between sex and PRS was seen on cognitive task performance, and sex-stratified analyses showed that the effect of PRS was male-specific. In a sub-sample, we observed a male-specific effect of the PRS on school performance at age 12 (N = 496). Our findings of sex-specific effects of schizophrenia genetics on cognitive functioning across the lifespan indicate that the effects of underlying disease genetics on cognitive functioning is dependent on biological processes that differ between the sexes.
Familial cleft tongue caused by a unique translation initiation codon variant in TP63
Authors: Schmidt Julia , Schreiber Gudrun , Altmüller Janine , Thiele Holger , Nürnberg Peter , Li Yun , Kaulfuß Silke , Funke Rudolf , Wilken Bernd , Yigit Gökhan , Wollnik Bernd
Published: October 2021

Variants in transcription factor p63 have been linked to several autosomal dominantly inherited malformation syndromes. These disorders show overlapping phenotypic characteristics with various combinations of the following features: ectodermal dysplasia, split-hand/foot malformation/syndactyly, lacrimal duct obstruction, hypoplastic breasts and/or nipples, ankyloblepharon filiforme adnatum, hypospadias and cleft lip/palate. We describe a family with six individuals presenting with a striking novel phenotype characterized by a furrowed or cleft tongue, a narrow face, reddish hair, freckles and various foot deformities. Whole-exome sequencing (WES) identified a novel heterozygous variant, c.3G>T, inTP63affecting the translation initiation codon (p.1Met?). Sanger sequencing confirmed dominant inheritance of this unique variant in all six affected family members. In summary, our findings indicate that heterozygous variants inTP63affecting the first translation initiation codon result in a novel phenotype dominated by a cleft tongue, expanding the complex genotypic and phenotypic spectrum ofTP63-associated disorders.
COVID-19: Impact on linguistic and genetic isolates of India
Authors: Singh Prajjval Pratap , Suravajhala Prashanth , Basu Mallick Chandana , Tamang Rakesh , Rai Ashutosh Kumar , Machha Pratheusa , Singh Royana , Pathak Abhishek , Mishra Vijay Nath , Shrivastava Pankaj , Singh Keshav K. , Thangaraj Kumarasamy , Chaubey Gyaneshwer
Published: October 2021

The rapid expansion of coronavirus SARS-CoV-2 has impacted various ethnic groups all over the world. The burden of infectious diseases including COVID-19 are generally reported to be higher for the Indigenous people. The historical knowledge have also suggested that the indigenous populations suffer more than the general populations in the pandemic. Recently, it has been reported that the indigenous groups of Brazil have been massively affected by COVID-19. Series of studies have shown that many of the indigenous communities reached at the verge of extinction due to this pandemic. Importantly, South Asia also has several indigenous and smaller communities, that are living in isolation. Till date, despite the two consecutive waves in India, there is no report on the impact of COVID-19 for indigenous tribes. Since smaller populations experiencing drift may have greater risk of such pandemic, we have analysed Runs of Homozygosity (ROH) among South Asian populations and identified several populations with longer homozygous segments. The longer runs of homozygosity at certain genomic regions may increases the susceptibility for COVID-19. Thus, we suggest extreme careful management of this pandemic among isolated populations of South Asia.
Truly privacy-preserving federated analytics for precision medicine with multiparty homomorphic encryption
Authors: Froelicher David , Troncoso-Pastoriza Juan R. , Raisaro Jean Louis , Cuendet Michel A. , Sousa Joao Sa , Cho Hyunghoon , Berger Bonnie , Fellay Jacques , Hubaux Jean-Pierre
Published: October 2021

Using real-world evidence in biomedical research, an indispensable complement to clinical trials, requires access to large quantities of patient data that are typically held separately by multiple healthcare institutions. We propose FAMHE, a novel federated analytics system that, based on multiparty homomorphic encryption (MHE), enables privacy-preserving analyses of distributed datasets by yielding highly accurate results without revealing any intermediate data. We demonstrate the applicability of FAMHE to essential biomedical analysis tasks, including Kaplan-Meier survival analysis in oncology and genome-wide association studies in medical genetics. Using our system, we accurately and efficiently reproduce two published centralized studies in a federated setting, enabling biomedical insights that are not possible from individual institutions alone. Our work represents a necessary key step towards overcoming the privacy hurdle in enabling multi-centric scientific collaborations.
Electro-optic spatial light modulator from an engineered organic layer
Authors: Benea-Chelmus Ileana-Cristina , Meretska Maryna L. , Elder Delwin L. , Tamagnone Michele , Dalton Larry R. , Capasso Federico
Published: October 2021

Tailored nanostructures provide at-will control over the properties of light, with applications in imaging and spectroscopy. Active photonics can further open new avenues in remote monitoring, virtual or augmented reality and time-resolved sensing. Nanomaterials with χ(2) nonlinearities achieve highest switching speeds. Current demonstrations typically require a trade-off: they either rely on traditional χ(2) materials, which have low non-linearities, or on application-specific quantum well heterostructures that exhibit a high χ(2) in a narrow band. Here, we show that a thin film of organic electro-optic molecules JRD1 in polymethylmethacrylate combines desired merits for active free-space optics: broadband record-high nonlinearity (10-100 times higher than traditional materials at wavelengths 1100-1600 nm), a custom-tailored nonlinear tensor at the nanoscale, and engineered optical and electronic responses. We demonstrate a tuning of optical resonances by Δλ = 11 nm at DC voltages and a modulation of the transmitted intensity up to 40%, at speeds up to 50 MHz. We realize 2 × 2 single- and 1 × 5 multi-color spatial light modulators. We demonstrate their potential for imaging and remote sensing. The compatibility with compact laser diodes, the achieved millimeter size and the low power consumption are further key features for laser ranging or reconfigurable optics.
Structural mechanism of GTPase-powered ribosome-tRNA movement
Authors: Petrychenko Valentyn , Peng Bee-Zen , de A. P. Schwarzer Ana C. , Peske Frank , Rodnina Marina V. , Fischer Niels
Published: October 2021

GTPases are regulators of cell signaling acting as molecular switches. The translational GTPase EF-G stands out, as it uses GTP hydrolysis to generate force and promote the movement of the ribosome along the mRNA. The key unresolved question is how GTP hydrolysis drives molecular movement. Here, we visualize the GTPase-powered step of ongoing translocation by time-resolved cryo-EM. EF-G in the active GDP–Pi form stabilizes the rotated conformation of ribosomal subunits and induces twisting of the sarcin-ricin loop of the 23 S rRNA. Refolding of the GTPase switch regions upon Pi release initiates a large-scale rigid-body rotation of EF-G pivoting around the sarcin-ricin loop that facilitates back rotation of the ribosomal subunits and forward swiveling of the head domain of the small subunit, ultimately driving tRNA forward movement. The findings demonstrate how a GTPase orchestrates spontaneous thermal fluctuations of a large RNA-protein complex into force-generating molecular movement.
Self-regeneration of supported transition metals by a high entropy-driven principle
Authors: Hou Shengtai , Ma Xuefeng , Shu Yuan , Bao Jiafeng , Zhang Qiuyue , Chen Mingshu , Zhang Pengfei , Dai Sheng
Published: October 2021

The sintering of Supported Transition Metal Catalysts (STMCs) is a core issue during high temperature catalysis. Perovskite oxides as host matrix for STMCs are proven to be sintering-resistance, leading to a family of self-regenerative materials. However, none other design principles for self-regenerative catalysts were put forward since 2002, which cannot satisfy diverse catalytic processes. Herein, inspired by the principle of high entropy-stabilized structure, a concept whether entropy driving force could promote the self-regeneration process is proposed. To verify it, a high entropy cubic Zr0.5(NiFeCuMnCo)0.5Ox is constructed as a host model, and interestingly in situ reversible exsolution-dissolution of supported metallic species are observed in multi redox cycles. Notably, in situ exsolved transition metals from high entropy Zr0.5(NiFeCuMnCo)0.5Ox support, whose entropic contribution (TΔSconfig = T⋆12.7 J mol−1 K−1) is predominant in ∆G, affording ultrahigh thermal stability in long-term CO2 hydrogenation (400 °C, >500 h). Current theory may inspire more STWCs with excellent sintering-resistance performance.
High temperature superconductivity at FeSe/LaFeO3 interface
Authors: Song Yuanhe , Chen Zheng , Zhang Qinghua , Xu Haichao , Lou Xia , Chen Xiaoyang , Xu Xiaofeng , Zhu Xuetao , Tao Ran , Yu Tianlun , Ru Hao , Wang Yihua , Zhang Tong , Guo Jiandong , Gu Lin , Xie Yanwu , Peng Rui , Feng Donglai
Published: October 2021

Enormous enhancement of superconducting pairing temperature (Tg) to 65 K in FeSe/SrTiO3 has made it a spotlight. Despite the effort of interfacial engineering, FeSe interfaced with TiOx remains the unique case in hosting high Tg, hindering a decisive understanding on the general mechanism and ways to further improving Tg. Here we constructed a new high-Tg interface, single-layer FeSe interfaced with FeOx-terminated LaFeO3. Large superconducting gap and diamagnetic response evidence that the superconducting pairing can emerge near 80 K, highest amongst all-known interfacial superconductors. Combining various techniques, we reveal interfacial charge transfer and strong interfacial electron-phonon coupling (EPC) in FeSe/LaFeO3, showing that the cooperative pairing mechanism works beyond FeSe-TiOx. Intriguingly, the stronger interfacial EPC than that in FeSe/SrTiO3 is likely induced by the stronger interfacial bonding in FeSe/LaFeO3, and can explain the higher Tg according to recent theoretical calculations, pointing out a workable route in designing new interfaces to achieve higher Tg.
FBXL2 counteracts Grp94 to destabilize EGFR and inhibit EGFR-driven NSCLC growth
Authors: Niu Mengmeng , Xu Jing , Liu Yang , Li Yuhuang , He Tao , Ding Liangping , He Yajun , Yi Yong , Li Fengtian , Guo Rongtian , Gao Ya , Li Rui , Li Luping , Fu Mengyuan , Hu Qingyong , Luo Yangkun , Zhang Chunyan , Qin Kewei , Yi Jianqiao , Yu Shuhan , Yang Jian , Chen Hu , Wang Liang , Li Zhonghan , Dong Biao , Qi Shiqian , Ouyang Liang , Zhang Yujun , Cao Yang , Xiao Zhi-Xiong Jim
Published: October 2021

Abnormal activation of epidermal growth factor receptor (EGFR) drives non-small cell lung cancer (NSCLC) development. EGFR mutations-mediated resistance to tyrosine-kinase inhibitors (TKIs) is a major hurdle for NSCLC treatment. Here, we show that F-box protein FBXL2 targets EGFR and EGFR TKI-resistant mutants for proteasome-mediated degradation, resulting in suppression of EGFR-driven NSCLC growth. Reduced FBXL2 expression is associated with poor clinical outcomes of NSCLC patients. Furthermore, we show that glucose-regulated protein 94 (Grp94) protects EGFR from degradation via blockage of FBXL2 binding to EGFR. Moreover, we have identified nebivolol, a clinically used small molecule inhibitor, that can upregulate FBXL2 expression to inhibit EGFR-driven NSCLC growth. Nebivolol in combination with osimertinib or Grp94-inhibitor-1 exhibits strong inhibitory effects on osimertinib-resistant NSCLC. Together, this study demonstrates that the FBXL2-Grp94-EGFR axis plays a critical role in NSCLC development and suggests that targeting FBXL2-Grp94 to destabilize EGFR may represent a putative therapeutic strategy for TKI-resistant NSCLC.
Polyphenolics profile effects upon the antioxidant and antimicrobial activity of propolis extracts
Authors: Nichitoi Mădălina Maria , Josceanu Ana Maria , Isopescu Raluca Daniela , Isopencu Gabriela Olimpia , Geana Elisabeta-Irina , Ciucure Corina Teodora , Lavric Vasile
Published: October 2021

Propolis, a complex bee product, is a source of numerous bioactive principles, beneficial for human health, therefore it is intensively studied. In the present work, extracts of propolis from Bihor Romanian County were studied to identify the relationship between the polyphenolic derivatives profile and their antioxidant and antimicrobial activity. Extracts were obtained using water and 25%, 50%, and 70% ethanolic solutions (w/w), at 2:1, 4:1, and 6:1 liquid: solid ratios (w/w). 21 polyphenolic derivatives were quantified by UHPLC-MS, proving that the extracts composition strongly depends on the solvent. The sum of quantified polyphenolics extracted varied between 1.5 and 91.2 mg/g propolis. The antioxidant capacity was evaluated using the free radicals 2,2’-azino-bis (3-ethylbenzothiazoline-6 sulfonic acid) diammonium salt (ABTS) and 1-diphenyl-2-picryl-hydrazyl (DPPH) scavenging methods. Antimicrobial efficiency was tested against Gram-positive (B. subtilis), Gram-negative bacteria (E. coli), and fungi (C. albicans) by disc-diffusion method. All extracts, even the aqueous ones, demonstrated antibacterial and antifungal activity. Chemometric methods (partial least squares) and a saturation-type model were used to evaluate the contribution of various bioactive principles in building the antioxidant capacity of extracts. Both experimental and modelling results show that 50% ethanolic extracts provide a rich polyphenolics profile and ensure a good antioxidant capacity.
Age constraints for the Trachilos footprints from Crete
Authors: Kirscher Uwe , El Atfy Haytham , Gärtner Andreas , Dallanave Edoardo , Munz Philipp , Niedźwiedzki Grzegorz , Athanassiou Athanassios , Fassoulas Charalampos , Linnemann Ulf , Hofmann Mandy , Bennett Matthew , Ahlberg Per Erik , Böhme Madelaine
Published: October 2021

We present an updated time frame for the 30 m thick late Miocene sedimentary Trachilos section from the island of Crete that contains the potentially oldest hominin footprints. The section is characterized by normal magnetic polarity. New and published foraminifera biostratigraphy results suggest an age of the section within the Mediterranean biozone MMi13d, younger than ~ 6.4 Ma. Calcareous nannoplankton data from sediments exposed near Trachilos and belonging to the same sub-basin indicate deposition during calcareous nannofossil biozone CN9bB, between 6.023 and 6.727 Ma. By integrating the magneto- and biostratigraphic data we correlate the Trachilos section with normal polarity Chron C3An.1n, between 6.272 and 6.023 Ma. Using cyclostratigraphic data based on magnetic susceptibility, we constrain the Trachilos footprints age at ~ 6.05 Ma, roughly 0.35 Ma older than previously thought. Some uncertainty remains related to an inaccessible interval of ~ 8 m section and the possibility that the normal polarity might represent the slightly older Chron C3An.2n. Sediment accumulation rate and biostratigraphic arguments, however, stand against these points and favor a deposition during Chron C3An.1n.
Communication processes about predictive genetic testing within high-risk breast cancer families: a two-phase study design
Authors: Blomen Chiara L. , Pott Aliaksandra , Volk Alexander E. , Budäus Lars , Witzel Isabell
Published: October 2021

The detection of a pathogenic variant in the BRCA1 or BRCA2 gene has medical and psychological consequences for both, affected mutation carriers and their relatives. A two-phase study with explanatory sequential mixed methods design examined the psychological impact of genetic testing and associated family communication processes. Analyzing a survey data of 79 carriers of a BRCA1 or BRCA2 mutation, the majority had general psychological distress independent of cancer diagnosis in the patients’ history. The point prevalence of depression was 16.9%. Contrary to their subjective perception, the respondents’ knowledge about those mutations was moderate. Despite the high rate of information transfer to relatives at risk (100%), their reported uptake of genetic testing was low (45.6%). Communication about the mutation detection was more frequent with female than with male relatives. In-depth focus group interviews revealed significant barriers to accessing genetic counseling including anxiety, uncertainty about the benefits of testing and about the own cancer risk, particularly among males. This study suggests that an adequate knowledge of the genetic background and psychological support is required to reduce emotional distress, to support familial communication and to facilitate genetic testing.
Impact of target site mutations and plasmid associated resistance genes acquisition on resistance of Acinetobacter baumannii to fluoroquinolones
Authors: Mohammed Mostafa Ahmed , Salim Mohammed T. A. , Anwer Bahaa E. , Aboshanab Khaled M. , Aboulwafa Mohammad M.
Published: October 2021

Among bacterial species implicated in hospital-acquired infections are the emerging Pan-Drug Resistant (PDR) and Extensively Drug-Resistant (XDR) Acinetobacter (A.) baumannii strains as they are difficult to eradicate. From 1600 clinical specimens, only 100 A. baumannii isolates could be recovered. A high prevalence of ≥ 78% resistant isolates was recorded for the recovered isolates against a total of 19 tested antimicrobial agents. These isolates could be divided into 12 profiles according to the number of antimicrobial agents to which they were resistant. The isolates were assorted as XDR (68; 68%), Multi-Drug Resistant (MDR: 30; 30%), and PDR (2; 2%). Genotypically, the isolates showed three major clusters with similarities ranging from 10.5 to 97.8% as revealed by ERIC-PCR technique. As a resistance mechanism to fluoroquinolones (FQs), target site mutation analyses in gyrA and parC genes amplified from twelve selected A. baumannii isolates and subjected to sequencing showed 12 profiles. The selected isolates included two CIP-susceptible ones, these showed the wild-type profile of being have no mutations. For the ten selected CIP-resistant isolates, 9 of them (9/10; 90%) had 1 gyrA/1 parC mutations (Ser 81 → Leu mutation for gyrA gene and Ser 84 → Leu mutation for parC gene). The remaining CIP-resistant isolate (1/10; 10%) had 0 gyrA/1 parC mutation (Ser 84 → Leu mutation for parC gene). Detection of plasmid-associated resistance genes revealed that the 86 ciprofloxacin-resistant isolates carry qnrA (66.27%; 57/86), qnrS (70.93%; 61/86), aac (6')-Ib-cr (52.32%; 45/86), oqxA (73.25%; 63/86) and oqxB (39.53%; 34/86), while qepA and qnrB were undetected in these isolates. Different isolates were selected from profiles 1, 2, and 3 and qnrS, acc(6,)-ib-cr, oqxA, and oqxB genes harbored by these isolates were amplified and sequenced. The BLAST results revealed that the oqxA and oqxB sequences were not identified previously in A. baumannii but they were identified in Klebsiella aerogenes strain NCTC9793 and Klebsiella pneumoniae, respectively. On the other hand, the sequence of qnrS, and acc(6,)-ib-cr showed homology to those of A. baumannii. MDR, XDR, and PDR A. baumannii isolates are becoming prevalent in certain hospitals. Chromosomal mutations in the sequences of GyrA and ParC encoding genes and acquisition of PAFQR encoding genes (up to five genes per isolate) are demonstrated to be resistance mechanisms exhibited by fluoroquinolones resistant A. baumannii isolates. It is advisable to monitor the antimicrobial resistance profiles of pathogens causing nosocomial infections and properly apply and update antibiotic stewardship in hospitals and outpatients to control infectious diseases and prevent development of the microbial resistance to antimicrobial agents.
Attenuated Salmonella carrying plasmid co-expressing HPV16 L1 and siRNA-E6 for cervical cancer therapy
Authors: Chen Junyu , Zhao Shuhua , Tan Wenxi , Wang Taiwei , Wu Shan , Wang Changshuai , Jiang Yu , Zhou Tuo , Zhang Zhuo , Zhao Lijing
Published: October 2021

Human papillomavirus (HPV) infection is the major etiological factor for cervical cancer. HPV prophylactic vaccines based on L1 virus-like particles have been considered as an effective prevention method. However, existing recombination vaccines are too expensive for developing countries. DNA vaccines might be a lower-cost and effective alternative. In this study, a plasmid (pcDNA3.1-HPV16-L1) and a co-expressing plasmid (pcDNA3.1-HPV16-L1-siE6) carried by attenuated Salmonella were constructed and their prevention and treatment effect on cervical cancer were observed, respectively. The results showed that pcDNA3.1-HPV16-L1 carried by attenuated Salmonella could induce the production of HPV16-L1 antibodies, IL-2 and INF-γ in mice serum, which presented its prevention effect on HPV. Subsequently, E6 and E7 gene silencing by pCG-siE6 inhibited the growth of cervical cancer both in vitro and in vivo. Furthermore, L1 up-regulation and E6/E7 down-regulation caused by co-expressing plasmid (pcDNA3.1-HPV16-L1-siE6) contributed to a significant anti-tumor effect on the mice. This study suggests that pcDNA3.1-HPV16-L1-siE6 carried by attenuated Salmonella has a synergistic effect of immune regulation and RNA interference in cervical cancer treatment.
The three muscle layers in the pyloric sphincter and their possible function during antropyloroduodenal motility
Authors: Hur Mi-Sun , Lee Seunggyu , Kang Tong Mook , Oh Chang-Seok
Published: October 2021

This study was conducted to determine the muscular arrangement of the human pyloric sphincter using a comprehensive approach that involved microdissection, histology, and microcomputed tomography (micro‐CT). The stomachs of 80 embalmed Korean adult cadavers were obtained. In all specimens, loose muscular tissue of the innermost aspect of the sphincter wall ran aborally, forming the newly found inner longitudinal muscle bundles, entered the duodenum, and connected with the nearby circular bundles. In all specimens, approximately one-third of the outer longitudinal layer of the sphincter entered its inner circular layer, divided the circular layer into several parts, and finally connected with the circular bundles. Anatomical findings around the sphincter were confirmed in micro-CT images. The sphincter wall comprised three layers: an inner layer of longitudinal bundles, a middle layer of major circular and minor longitudinal bundles, and an outer layer of longitudinal bundles. The stomach outer longitudinal bundles were connected to the sphincter circular bundles. The inner longitudinal bundles of the sphincter were connected to the adjacent circular bundles of the duodenum.
Constraining the axion–photon coupling using radio data of the Bullet cluster
Authors: Chan Man Ho
Published: October 2021

Axion is one of the most popular candidates of the cosmological dark matter. Recent studies considering the misalignment production of axions suggest some benchmark axion mass ranges near $$m_a \sim 20$$

m
a

∼
20

μeV. For such axion mass, the spontaneous decay of axions can give photons in radio band frequency $$\nu \sim 1{-}3$$

ν
∼
1
-
3

GHz, which can be detected by radio telescopes. In this article, we show that using radio data of galaxy clusters would be excellent to constrain axion dark matter. Specifically, by using radio data of the Bullet cluster (1E 0657-55.8), we find that the upper limit of the axion–photon coupling constant can be constrained to $$g_{a \gamma \gamma } \sim 10^{-12}{-}10^{-11}$$

g

a
γ
γ

∼

10

-
12

-

10

-
11

GeV$$^{-1}$$

-
1

for $$m_a \sim 20$$

m
a

∼
20

μeV, which is tighter than the limit obtained by the CERN Axion Solar Telescope (CAST).
The contribution of semantics to the sentence superiority effect
Authors: Massol Stéphanie , Mirault Jonathan , Grainger Jonathan
Published: October 2021

When a sequence of written words is presented briefly and participants are asked to report the identity of one of the words, identification accuracy is higher when the words form a correct sentence. Here we examined the extent to which this sentence superiority effect can be modulated by semantic content. The central hypothesis guiding this study is that the sentence superiority effect is primarily a syntactic effect. We therefore predicted little or no modulation of the effect by semantics. The influence of semantic content was measured by comparing the sentence superiority effect obtained with semantically regular sentences (e.g., son amie danse bien [her friend dances well]) and semantically anomalous but syntactically correct sentences (e.g., votre sac boit gros [your bag drinks big]), with effects being measured against ungrammatical scrambled versions of the same words in both cases. We found sentence superiority effects with both types of sentences, and a significant interaction, such that the effects were greater with semantically regular sentences compared with semantically anomalous sentences. We conclude that sentence-level semantic information can constrain word identities under parallel word processing, albeit with less impact than that exerted by syntax.
Toy model of harmonic and sum frequency generation in 2D dielectric nanostructures
Authors: Xu Jie , Savinov Vassili , Plum Eric
Published: October 2021

Optical nonlinearities of matter are often associated with the response of individual atoms. Here, using a toy oscillator model, we show that in the confined geometry of a two-dimensional dielectric nanoparticle a collective nonlinear response of the atomic array can arise from the Coulomb interactions of the bound optical electrons, even if the individual atoms exhibit no nonlinearity. We determine the multipole contributions to the nonlinear response of nanoparticles and demonstrate that the odd order and even order nonlinear electric dipole moments scale with the area and perimeter of the nanoparticle, respectively.
The profile of microorganisms that associate with acute tonsillitis in children and their antibiotics sensitivity pattern in Nigeria
Authors: Ughasoro Maduka Donatus , Akpeh James Onuorah , Echendu Nneamaka , Mgbachi Nneka Gertrude , Okpala Somkene , Amah Linda , Okolo Onyinye Henrietta , Udem Ngozika
Published: October 2021

Acute tonsillitis remains one of the common childhood diseases in developing countries. Prompt and appropriate treatment based on the knowledge of the causative microbiota and their antimicrobial susceptibility pattern will improve the treatment outcome and reduce time and resources spent on treatment. This study aims to determine the pattern of microbiota isolates and their susceptibility pattern. The study was a combination of the retrospective and cross-sectional method. The medical records of children treated for tonsillitis were retrieved, as well as those of children who presented with acute tonsillitis for the study. Interviewer-administered questionnaire was used to collect data, as well as document information retrieved from their medical record: presenting symptoms, treatments received. Swab sample was taken for culture and antibiotic susceptibility test. Out of the 72 swab cultures, 29 (40.3%) yielded insignificant growth of gram positive cocci. A total of nine (9) different isolates were obtained from all the significant swab cultures. Streptococcus species (13, 18.6%) and staphylococcus species (11, 15.3%) were the commonest isolates. Imipenem and levofloxacin revealed high level of susceptibility, while Ampicillin and Cefexine recorded high resistance rates. The proportion of the cultures that were positive of significant growth, the proportion of these positive isolates that were Streptococcus spp. and varied sensitivity pattern obtained underpinned the importance to advocate for culture isolates and susceptibility pattern guided treatment. These will not only an efficient approach to management of acute tonsillitis, but also a strong approach towards effective implementation of antibiotic stewardship.
Global processing provides malignancy evidence complementary to the information captured by humans or machines following detailed mammogram inspection
Authors: Gandomkar Ziba , Siviengphanom Somphone , Ekpo Ernest U. , Suleiman Mo’ayyad , Taba‬ Seyedamir Tavakoli , Li Tong , Xu Dong , Evans Karla K. , Lewis Sarah J. , Wolfe Jeremy M. , Brennan Patrick C.
Published: October 2021

The information captured by the gist signal, which refers to radiologists’ first impression arising from an initial global image processing, is poorly understood. We examined whether the gist signal can provide complementary information to data captured by radiologists (experiment 1), or computer algorithms (experiment 2) based on detailed mammogram inspection. In the first experiment, 19 radiologists assessed a case set twice, once based on a half-second image presentation (i.e., gist signal) and once in the usual viewing condition. Their performances in two viewing conditions were compared using repeated measure correlation (rm-corr). The cancer cases (19 cases × 19 readers) exhibited non-significant trend with rm-corr = 0.012 (p = 0.82, CI: −0.09, 0.12). For normal cases (41 cases × 19 readers), a weak correlation of rm-corr = 0.238 (p < 0.001, CI: 0.17, 0.30) was found. In the second experiment, we combined the abnormality score from a state-of-the-art deep learning-based tool (DL) with the radiological gist signal using a support vector machine (SVM). To obtain the gist signal, 53 radiologists assessed images based on half-second image presentation. The SVM performance for each radiologist and an average reader, whose gist responses were the mean abnormality scores given by all 53 readers to each image was assessed using leave-one-out cross-validation. For the average reader, the AUC for gist, DL, and the SVM, were 0.76 (CI: 0.62–0.86), 0.79 (CI: 0.63–0.89), and 0.88 (CI: 0.79–0.94). For all readers with a gist AUC significantly better than chance-level, the SVM outperformed DL. The gist signal provided malignancy evidence with no or weak associations with the information captured by humans in normal radiologic reporting, which involves detailed mammogram inspection. Adding gist signal to a state-of-the-art deep learning-based tool improved its performance for the breast cancer detection.
Unraveling the complexity of human behavior and urbanization on community vulnerability to floods
Authors: Hemmati Mona , Mahmoud Hussam N. , Ellingwood Bruce R. , Crooks Andrew T.
Published: October 2021

Floods are among the costliest natural hazards and their consequences are expected to increase further in the future due to urbanization in flood-prone areas. It is essential that policymakers understand the factors governing the dynamics of urbanization to adopt proper disaster risk reduction techniques. Peoples’ relocation preferences and their perception of flood risk (collectively called human behavior) are among the most important factors that influence urbanization in flood-prone areas. Current studies focusing on flood risk assessment do not consider the effect of human behavior on urbanization and how it may change the nature of the risk. Moreover, flood mitigation policies are implemented without considering the role of human behavior and how the community will cope with measures such as buyout, land acquisition, and relocation that are often adopted to minimize development in flood-prone regions. Therefore, such policies may either be resisted by the community or result in severe socioeconomic consequences. In this study, we present a new Agent-Based Model (ABM) to investigate the complex interaction between human behavior and urbanization and its role in creating future communities vulnerable to flood events. We identify critical factors in the decisions of households to locate or relocate and adopt policies compatible with human behavior. The results show that when people are informed about the flood risk and proper incentives are provided, the demand for housing within 500-year floodplain may be reduced as much as 15% by 2040 for the case study considered. On the contrary, if people are not informed of the risk, 29% of the housing choices will reside in floodplains. The analyses also demonstrate that neighborhood quality—influenced by accessibility to highways, education facilities, the city center, water bodies, and green spaces, respectively—is the most influential factor in peoples’ decisions on where to locate. These results provide new insights that may be used to assist city planners and stakeholders in examining tradeoffs between costs and benefits of future land development in achieving sustainable and resilient cities.
Assessment of the relationship between serum uric acid levels and liver enzymes activity in Bangladeshi adults
Authors: Molla Noyan Hossain , Kathak Rahanuma Raihanu , Sumon Abu Hasan , Barman Zitu , Mou Ananya Dutta , Hasan Akibul , Mahmud Firoz , Fariha Khandaker Atkia , Ali Nurshad
Published: October 2021

Serum uric acid (SUA) level has been suggested to be associated with cardiovascular disease, diabetes and metabolic syndrome. However, little is known about the relationship between SUA and liver enzymes activity in the general population. The present study aimed to assess the relationship between SUA and serum liver enzymes in an adult population in Bangladesh. In this cross-sectional study, a total of 410 blood samples were collected from apparently healthy adults aged > 18 years. SUA, liver enzymes, lipid profile and other biochemical markers were measured in the collected samples by using standard methods. Multinomial logistic regression model was used to assess the relationship between SUA and elevated levels of liver enzymes among the participants. Overall, the prevalence of hyperuricemia was 30.1% with 32.2% in male and 18.6% in female participants. About 33% of the participants had at least one or more elevated levels of liver enzymes. The mean level of SUA was significantly higher in males (389.3 ± 96.9 µmol/L) than in the female (290.4 ± 89.8 µmol/L) subjects (p < 0.001). There was a significant difference in the mean levels of serum ALT and GGT between the male (34.5 ± 16.0 U/L and 26.7 ± 19.5 U/L, respectively) and female (25.0 ± 13.0 U/L and 19.5 ± 13.2 U/L, respectively) participants (p < 0.001 and p < 0.01, respectively). An increasing trend was observed in the mean levels of serum ALT and GGT across the SUA quartile groups (p < 0.001 and p < 0.01, respectively). SUA showed a positive and significant correlation with serum ALT (p < 0.001) and GGT (p < 0.01). In further statistical analysis after adjustment for potential confounders, SUA showed an independent and significant association with serum ALT and GGT in all regression models. In conclusion, SUA was strongly associated with serum levels of ALT and GGT after adjustment for potential confounders. More prospective studies are needed to clarify the complex relationship between SUA and liver enzymes in the general population.
Real world experience of the treatment outcome between photodynamic therapy combined with ranibizumab and aflibercept monotherapy in polypoidal choroidal vasculopathy
Authors: Ma I-Hsin , Hsia Yun , Hsieh Yi-Ting , Ho Tzyy-Chang , Lai Tso-Ting , Yang Chung-May , Yang Chang-Hao
Published: October 2021

To provide real-world experiences of treating polypoidal choroidal vasculopathy (PCV) patients with photodynamic therapy (PDT) plus intravitreal injection of ranibizumab or intravitreal injection of aflibercept alone. Retrospective chart review of patients with PCV in a single tertiary referral center in Taiwan. Chart review of PCV patients treated with PDT and injection of ranibizumab or injection of aflibercept. A total of 101 eyes of 101 patients (38 females and 63 males) were reviewed. Of those, 48 and 53 eyes received primary/adjunctive PDT along with injections of ranibizumab or intravitreal injections of aflibercept only, respectively. Initial visual acuity (VA) and central subfield choroidal thickness were similar between the two groups (p > 0.05). In addition, changes in VA at 3, 6, and 12 months post treatment were similar. The central retinal thickness decreased with either treatment (p < 0.01); however, this change did not translate into VA performance (p > 0.05). In the subgroup analysis of pachychoroid and non-pachychoroid patients, better initial VA and post-treatment VA at 3 months and 6 months was noted in the latter group of patients treated with anti-vascular endothelial growth factor monotherapy (p < 0.05). Aflibercept monotherapy is comparable with PDT plus ranibizumab in PCV patients with PCV (pachychoroid and non-pachychoroid patients). In addition, better prognosis regarding VA was observed in non-pachychoroid patients treated with aflibercept monotherapy.
Extracellular matrix and cellular senescence in venous leg ulcers
Authors: Lim Debbie X. E. , Richards Toby , Kanapathy Muholan , Sudhaharan Thankiah , Wright Graham D. , Phillips Anthony R. J. , Becker David L.
Published: October 2021

High prevalence of non-healing chronic wounds contributes to a huge healthcare burden across the world. Early treatment interventions for non-healing wounds are vital. It was previously shown that accumulation of 15% or more of senescent cells in a chronic wound edge is an indicator that the wound is unlikely to heal. However, determining the presence of senescent cells would require invasive procedures such as tissue biopsies to be taken. In this study, we found a strong correlation between decreased collagen area and presence of senescent cells in human chronic wounds i.e. venous leg ulcer (VLU), diabetic foot ulcer (DFU) and pressure ulcer (PRU). We also report that the lowest collagen levels were found in VLU patients less than 60 years of age, with a persistent wound of > 24 months. Elevated levels of senescent cells were also found in VLU of males. Second harmonic imaging of collagen at the edge of chronic wounds with a handheld multiphoton device could be used to predict the number of senescent cells, indicating if the wound is on a healing trajectory or not. Our data support the use of collagen imaging in cutaneous wound assessment for a faster and non-invasive method to predict cellular senescence and determining wound trajectory of healing.
A comparison of the clinical, laboratory and epidemiological features of two divergent subpopulations of Plasmodium knowlesi
Authors: Hu Ting Huey , Rosli Nawal , Mohamad Dayang S. A. , Kadir Khamisah A. , Ching Zhen Hao , Chai Yaw Hung , Ideris Nur Naqibah , Ting Linda S. C. , Dihom Adeline A. , Kong Sing Ling , Wong Edmund K. Y. , Sia Jenny E. H. , Ti Tiana , Chai Irene P. F. , Tang Wei Yieng , Hii King Ching , Divis Paul C. S. , Davis Timothy M. E. , Daneshvar Cyrus , Singh Balbir
Published: October 2021

Plasmodium knowlesi, a simian malaria parasite responsible for all recent indigenous cases of malaria in Malaysia, infects humans throughout Southeast Asia. There are two genetically distinct subpopulations of Plasmodium knowlesi in Malaysian Borneo, one associated with long-tailed macaques (termed cluster 1) and the other with pig-tailed macaques (cluster 2). A prospective study was conducted to determine whether there were any between-subpopulation differences in clinical and laboratory features, as well as in epidemiological characteristics. Over 2 years, 420 adults admitted to Kapit Hospital, Malaysian Borneo with knowlesi malaria were studied. Infections with each subpopulation resulted in mostly uncomplicated malaria. Severe disease was observed in 35/298 (11.7%) of single cluster 1 and 8/115 (7.0%) of single cluster 2 infections (p = 0.208). There was no clinically significant difference in outcome between the two subpopulations. Cluster 1 infections were more likely to be associated with peri-domestic activities while cluster 2 were associated with interior forest activities consistent with the preferred habitats of the respective macaque hosts. Infections with both P. knowlesi subpopulations cause a wide spectrum of disease including potentially life-threatening complications, with no implications for differential patient management.
Hysteresis of heavy metals uptake induced in Taraxacum officinale by thiuram
Authors: Adamczyk-Szabela Dorota , Lisowska Katarzyna , Wolf Wojciech M.
Published: October 2021

Dandelion (Taraxacum officinale) yields active substances frequently used in herbal medicinal preparations. Its plantations are exposed to fungal plagues which pose a threat to herbal crops. The aim of this study was to evaluate the long time effects of a fungicide thiuram on dandelion growth and photosynthesis. Additionally, the manganese, iron, copper, zinc, cadmium, and lead uptake and transport were also investigated. Plants were cultivated under greenhouse conditions by the pot method in a universal flowering soil. The elements content in soil and plants were determined by the HR-CS FAAS spectrometer. Thiuram concentrations were established by the HPLC. Those analyses showed that almost 80% of thiuram decomposed within two weeks of its application. The photosynthesis indicators suggested, that plants were in good conditions and the fungicide supplementation facilitated plant growth. The latter could be prompted by thiuram acting as a sulfur rich chemical micro fertilizer. The hypothesis, that thiuram significantly affects heavy metals interactions in dandelion was proved by the one-way analysis of variance. Notable, metals uptake did not completely recover after fungicide decomposition for all investigated elements except iron We suggest to define this chemically induced, time-dependent heavy metals migrations in the soil–plant system as hysteresis of heavy metals uptake.
Enhanced anomalous magnetization in carbonyl iron by Ni+ ion beam irradiation
Authors: Park Jun Kue , Jang Hye Min , Cho Won-Je , Kim Chorong , Suk Jaekwon , Kim Dong-Seok , Lee Jae Sang
Published: October 2021

We investigate the magnetic properties in carbonyl iron (CI) particles before and after Ni$$^{+}$$

+

and H$$^{+}$$

+

ion beam irradiation. Upon increasing temperatures, the saturation magnetization ($$M_{\text {s}}$$

M
s

) in hysteresis loops exhibits an anomalous increase at a high temperature for the unirradiated and the Ni$$^{+}$$

+

-beam-irradiated samples, unlike in H$$^{+}$$

+

-beam-irradiated sample. Moreover, the magnetization values at low and high temperatures are more intense after Ni$$^{+}$$

+

beam irradiation, whereas after H$$^{+}$$

+

beam irradiation those are remarkably suppressed. Hematite ($$\alpha $$
α
-Fe$$_{2}$$

2

O$$_{3}$$

3

) phase introduced on the surface of our CI particles undergoes the Morin transition that was observed in our magnetization-temperature curves. The Morin transition causing canted antiferromagnetism above the Morin temperature was found in the unirradiated and Ni$$^{+}$$

+

-beam-irradiated samples, but not in H$$^{+}$$

+

-beam-irradiated sample. It is thus revealed that the CI particles undergoing the Morin transition cause the anomalous increase in $$M_{\text {s}}$$

M
s

. We may suggest that Ni$$^{+}$$

+

ion beam increases uncompensated surface spins on the CI particles resulting in a more steep Morin transition and the intensified $$M_{\text {s}}$$

M
s

. Ion-beam irradiation may thus be a good tool for controlling the magnetic properties of CI particles, tailoring our work for future applications.
Cardiorespiratory function of patients undergoing surgical correction of Scheuermann's hyperkyphosis
Authors: Vera Pablo , Lorente Alejandro , Burgos Jesús , Palacios Pablo , Antón-Rodrigálvarez Luis M. , Tamariz Rocio , Barrios Carlos , Lorente Rafael
Published: October 2021

The aim of this study was to analyze the impact of surgical correction of the thoracic deformity on the cardiorespiratory function of patients with moderate-severe Scheuermann’s hyperkyphosis (SK). A series of 23 adolescents with SK who underwent surgery through an only posterior approach using all pedicle screw constructs were included in the study. Cardiorespiratory parameters were measured during a maximal exercise tolerance test before and 2 years after surgery. Heart rate, oxygen saturation (SatO2), maximum oxygen uptake (VO2 max), ventilatory capacity at maximal exercise (VEmax), and energy costs were recorded. There were statistically significant differences in the forced vital capacity (FVC) (P < 0.05), total VO2max (ml/min) (P < 0.01), maximum expired volume (VEmax) per minute (P < 0.01) and cardiovascular efficiency (HR/VO2 ratio) (P < 0.05). None of these changes were clinically relevant. There were no changes in the VO2max per kg of body mass. The magnitude of the kyphosis correction did not correlate with the change in normalized VO2max or VEmax. In conclusion, patients with moderate-severe SK improve their baseline respiratory limitations and the tolerance to maximum exercise 2 years after surgery. However, the slight cardiorespiratory functional improvements should not necessarily be attributed to the surgery, and could also be caused solely by the residual growth of the lungs and thorax. Furthermore, respiratory functional changes are under thresholds considered as clinically relevant.