bluuebunny/crossref_metadata_2025 huggingface dataset:
The correlation of gait and muscle activation characteristics with locomotion dysfunction grade in elderly individuals
Authors: Liu Wen , Bai Jinzhu
Published: August 2024

Objective

To investigate the differences and regularity of gait and muscle activation characteristics parameters in the Locomotion Dysfunction Grade (LDG) scale assessment in elderly individuals, and analyse the correlation between objective parameters and scale grading. Thus, to propose a novel detection mode for elderly individuals, which combined the LDG scale with objective detection. It can not only provide quantitative data for intelligent evaluation and rehabilitation, but also provided more accurate reference for the classification of care levels in elderly care policies.

Methods

Elderly individuals (n = 159) who underwent gait analysis and sEMG at the Chinese Rehabilitation Research Center from January 2019 to September 2023 were included. According to the LDG scale, the elderly individuals were divided into four groups, namely, the LDG4, LDG5, LDG6 groups and the healthy control group. Four indicators, namely, spatiotemporal, kinematic, dynamic gait parameters and muscle activation characteristics data, were collected. Changes in these characteristics of elderly individuals with lower extremity motor dysfunction were evaluated and analysed statistically.

Results

The spatiotemporal gait parameters were significantly lower in the LDG4, LDG5, LDG6 groups than in the healthy control group. The double support phase was positively correlated with the LDG, while the swing phase, step length and velocity were negatively correlated (P < 0.05). The movement angles of both hips, knees and ankles were significantly limited and negatively correlated with the LDG (P < 0.05). Compared with those in the healthy control group, the centre of pressure (COP) path length were greater, and the average COP velocity was significantly lower (P < 0.05) in the LDG4, LDG5, LDG6 groups. The regularity of muscle activation clearly changed. The root mean square of the gastrocnemius medialis was positively correlated with LDG (P < 0.05), while the tibialis anterior showed no regularity.

Conclusion

As the LDG increased, the differences in spatiotemporal, kinematic and dynamic gait parameters between elderly individuals with motor dysfunction and the healthy individuals gradually increased. The muscle activation characteristics parameters showed an abnormal activation pattern. These parameters were correlated with the LDG, providing a more comprehensive and objective assessment of lower extremity motor function in elderly individuals, improve assessment accuracy, and help accurate rehabilitation.
MARes-Net: multi-scale attention residual network for jaw cyst image segmentation
Authors: Ding Xiaokang , Jiang Xiaoliang , Zheng Huixia , Shi Hualuo , Wang Ban , Chan Sixian
Published: August 2024

Jaw cyst is a fluid-containing cystic lesion that can occur in any part of the jaw and cause facial swelling, dental lesions, jaw fractures, and other associated issues. Due to the diversity and complexity of jaw images, existing deep-learning methods still have challenges in segmentation. To this end, we propose MARes-Net, an innovative multi-scale attentional residual network architecture. Firstly, the residual connection is used to optimize the encoder-decoder process, which effectively solves the gradient disappearance problem and improves the training efficiency and optimization ability. Secondly, the scale-aware feature extraction module (SFEM) significantly enhances the network’s perceptual abilities by extending its receptive field across various scales, spaces, and channel dimensions. Thirdly, the multi-scale compression excitation module (MCEM) compresses and excites the feature map, and combines it with contextual information to obtain better model performance capabilities. Furthermore, the introduction of the attention gate module marks a significant advancement in refining the feature map output. Finally, rigorous experimentation conducted on the original jaw cyst dataset provided by Quzhou People’s Hospital to verify the validity of MARes-Net architecture. The experimental data showed that precision, recall, IoU and F1-score of MARes-Net reached 93.84%, 93.70%, 86.17%, and 93.21%, respectively. Compared with existing models, our MARes-Net shows its unparalleled capabilities in accurately delineating and localizing anatomical structures in the jaw cyst image segmentation.
Comparison of clinical and echocardiographic outcomes between mini-thoracotomy transatrial LuX-Valve transcatheter and surgical tricuspid valve replacement
Authors: Huang Lei , Sun Zhenxing , Cai Yu , Xie Yuji , Zhang Ziming , Sun Wei , Li He , Fang Lingyun , He Lin , Zhang Li , Yang Yali , Wang Jing , Lv Qing , Li Yuman , Xie Mingxing
Published: August 2024

Background and aims

Transcatheter tricuspid valve replacement (TTVR) has recently emerged as a novel therapeutic approach for managing severe tricuspid regurgitation (TR). However, surgical tricuspid valve replacement (STVR) continues to be the predominant treatment modality. There are limited comparative data on both procedures. This study aimed to compare clinical and echocardiographic outcomes between patients who underwent mini-thoracotomy transatrial LuX-Valve TTVR and those who underwent STVR.

Methods

This study prospectively collected patients with severe TR who underwent TTVR (n = 29) or isolated STVR (n = 59) at Wuhan Union Hospital from 2019 to 2022. All TTVR patients received the LuX-Valve via a mini-thoracotomy and transatrial approach. The clinical and echocardiographic outcomes were compared at 30-day and one-year follow-ups.

Results

At baseline, patients with LuX-Valve TTVR had higher surgical risk scores and a greater proportion of right ventricular dysfunction compared with STVR. In the early postoperative period, the STVR group had a greater decrease in right ventricular function. Hospital length of stay (LOS), intensive care unit LOS, total procedure time, and tracheal intubation time were shorter in the TTVR than in the STVR group. The incidence of postoperative paravalvular leaks was higher among patients who underwent TTVR. Compared to the STVR group, the pacemaker implantation rate was lower in the TTVR group. During follow-up, the peak tricuspid valve velocity and mean gradient in the TTVR group were consistently lower than those in the STVR group. There was similar mortality between TTVR and STVR at 30-day and one-year follow-ups.

Conclusions

The mini-thoracotomy transatria LuX-Valve TTVR has a higher incidence of paravalvular leaks and a lower rate of pacemaker implantation than STVR, with similar 30-day and one-year mortality rates. In some respects, mini-thoracotomy transatrial LuX-Valve TTVR may be a feasible and safe treatment option for specific populations, or it could potentially serve as an alternative therapy to supplement conventional STVR. Further follow-up is required to assess differences in long-term clinical outcomes and valve durability.
“It actually helped”: students’ perceptions of feedback helpfulness prior to and following a teacher professional learning intervention
Authors: Brooks Cameron , Burton Rochelle , Van der Kleij Fabienne , Ablaza Christine , Carroll Annemaree , Hattie John , Salinas Jaime Garcia
Published: August 2024

This study investigated the effects of a teacher professional learning intervention, underpinned by a student-centred model of feedback, on student perceptions of feedback helpfulness. The study was conducted in the context of primary education English writing in Queensland, Australia. No overall differences in feedback perceptions of students in 13 intervention and 9 comparison schools were identified following the intervention. However, more detailed analyses revealed significantly greater increases in perceived helpfulness among intervention group students for six feedback strategies. This suggests the intervention changed teachers’ feedback practices, enhancing student perceptions of feedback helpfulness. Student focus group data provided valuable qualitative insights into student feedback perceptions. Overall findings highlight the interrelatedness between feedback strategies across the feedback cycle for enhancing student learning.
The function of phytogenic mounds in the accumulation and conservation of soil seed banks in semiarid areas with water erosion
Authors: Nie W. J. , Du H. D. , Xie S. S. , Bi Y. L.
Published: August 2024

Background

Phytogenic mounds are a type of microtopography formed under perennial plants canopies in water erosion areas. However, the function of phytogenic mounds in seed assemblages and their ecological consequences remain poorly understood in semiarid areas with water erosion. Thus, understanding the characteristics of seed banks on mounds is crucial for ecosystem conservation and management in water-eroded areas.

Methods

We compared the quantity and composition of soil seed banks on the upslope and downslope parts of mounds and intercanopy surfaces along four slope gradients. We also explored the relationships among the soil seed bank, aboveground vegetation, and environmental factors. Furthermore, the species similarity between the soil seed bank and aboveground vegetation was analyzed to clarify the important ecological consequences of phytogenic mounds for plant community construction in serious soil erosion area.

Results

For slopes with α ≤ 46.6%, the intercanopy surfaces had greater soil seed bank species composition, density, and diversity than did the phytogenic mounds, and these characteristics showed no significant differences between the upslope and downslope parts of the mounds. As the slope increased, the soil seed bank density and species composition increased on the upslope part of the mound, and reached a maximum for slopes with α > 70%, while the downslope part of the mound negatively effected on seed aggregation. The sediment accumulation rate, soil moisture, particle size distribution, pH, organic matter carbon, and hardness were significantly correlated with the soil seed bank density and diversity in the study area. For slopes with 0 < α ≤ 26.8%, the species similarity coefficient between the soil seed bank and aboveground vegetation was the highest for the intercanopy surface. This species similarity on the upslope part of the mound showed an increasing trend with increasing slope gradient, while the downslope part of the mound had the opposite trend. For slopes with α > 70%, the upslope part of the mound did not only have more species in the soil seed bank but also had more species in aboveground vegetation than did the downslope part of the mound and intercanopy surface.

Conclusion

For slopes with α ≤ 46.6%, phytogenic mounds had barely impact soil seed bank accumulation and conservation in semiarid and eroded areas. For slopes with α > 46.6%, the mounds (particularly on the upslope part of the mound) showed seed assemblage functions, which are coupled with improving edaphic conditions and decreasing microhabitat stress; thus, phytogenic mounds, or areas of microtopography, can be used to promote restoration success in semiarid eroded areas.
Native bee habitat restoration: key ecological considerations from recent North American literature
Authors: Payne Helen E. , Mazer Susan J. , Seltmann Katja C.
Published: August 2024

Habitat loss is a primary driver of global biodiversity decline, negatively impacting many species, including native bees. One approach to counteract the consequences of habitat loss is through restoration, which includes the transformation of degraded or damaged habitats to increase biodiversity. In this review, we survey bee habitat restoration literature over the last 14 years to provide insights into how best to promote bee diversity and abundance through the restoration of natural landscapes in North America. We highlight relevant questions and concepts to consider throughout the various stages of habitat restoration projects, categorizing them into pre-, during-, and post-restoration stages. We emphasize the importance of planning species- and site-specific strategies to support bees, including providing floral and non-floral resources and increasing nest site availability. Lastly, we underscore the significance of conducting evaluations and long-term monitoring following restoration efforts. By identifying effective restoration methods, success indicators, and areas for future research, our review presents a comprehensive framework that can guide land managers during this urgent time for bee habitat restoration.
Inhibition of insulin-like growth factors increases production of CXCL9/10 by macrophages and fibroblasts and facilitates CD8+ cytotoxic T cell recruitment to pancreatic tumours
Authors: Freeman Patrick , Bellomo Gaia , Ireland Lucy , Abudula Maidinaimu , Luckett Teifion , Oberst Michael , Stafferton Ruth , Ghaneh Paula , Halloran Chris , Schmid Michael C. , Mielgo Ainhoa
Published: August 2024

Pancreatic ductal adenocarcinoma (PDAC) is a highly lethal malignancy with an urgent unmet clinical need for new therapies. Using a combination of in vitro assays and in vivo preclinical models we demonstrate that therapeutic inhibition of the IGF signalling axis promotes the accumulation of CD8+ cytotoxic T cells within the tumour microenvironment of PDAC tumours. Mechanistically, we show that IGF blockade promotes macrophage and fibroblast production of the chemokines CXCL9 and CXCL10 to facilitate CD8+ T cell recruitment and trafficking towards the PDAC tumour. Exploring this pathway further, we show that IGF inhibition leads to increased STAT1 transcriptional activity, correlating with a downregulation of the AKT/STAT3 signalling axis, in turn promoting Cxcl9 and Cxcl10 gene transcription. Using patient derived tumour explants, we also demonstrate that our findings translate into the human setting. PDAC tumours are frequently described as “immunologically cold”, therefore bolstering CD8+ T cell recruitment to PDAC tumours through IGF inhibition may serve to improve the efficacy of immune checkpoint inhibitors which rely on the presence of CD8+ T cells in tumours.
Exploring the bone marrow micro environment in thalassemia patients: potential therapeutic alternatives
Authors: Li Zengzheng , Yao Xiangmei , Zhang Jie , Yang Jinghui , Ni Junxue , Wang Yajie
Published: August 2024

Genetic mutations in the β-globin gene lead to a decrease or removal of the β-globin chain, causing the build-up of unstable alpha-hemoglobin. This condition is referred to as beta-thalassemia (BT). The present treatment strategies primarily target the correction of defective erythropoiesis, with a particular emphasis on gene therapy and hematopoietic stem cell transplantation. However, the presence of inefficient erythropoiesis in BT bone marrow (BM) is likely to disturb the previously functioning BM microenvironment. This includes accumulation of various macromolecules, damage to hematopoietic function, destruction of bone cell production and damage to osteoblast(OBs), and so on. In addition, the changes of BT BM microenvironment may have a certain correlation with the occurrence of hematological malignancies. Correction of the microenvironment can be achieved through treatments such as iron chelation, antioxidants, hypoglycemia, and biologics. Hence, This review describes damage in the BT BM microenvironment and some potential remedies.
Mitochondria: a breakthrough in combating rheumatoid arthritis
Authors: Li Shuang , Huo Chenlu , Liu Anting , Zhu Yan
Published: August 2024

As a chronic autoimmune disease with complex aetiology, rheumatoid arthritis (RA) has been demonstrated to be associated with mitochondrial dysfunction since mitochondrial dysfunction can affect the survival, activation, and differentiation of immune and non-immune cells involved in the pathogenesis of RA. Nevertheless, the mechanism behind mitochondrial dysfunction in RA remains uncertain. Accordingly, this review addresses the possible role and mechanisms of mitochondrial dysfunction in RA and discusses the potential and challenges of mitochondria as a potential therapeutic strategy for RA, thereby providing a breakthrough point in the prevention and treatment of RA.
Green control for inhibiting Rhizopus oryzae growth by stress factors in forage grass factory
Authors: Dai Mengdi , Tan Xiangfeng , Chen Xuting , Cai Kangfeng , Zhong Yuanxiang , Ye Ziran , Kong Dedong
Published: August 2024

The forage grass factory could break through the restrictions of land resources, region and climate to achieve efficient production throughout the year by accurate and intelligent management. However, due to its closed environment, mold outbreaks in the forage grass factory were severe, significantly affecting barley production. In this study, 9 contaminated barley tissues were collected and 45 strains were isolated in forage grass factory. After ITS sequencing, 45 strains were all identified as Rhizopus oryzae. Through stress factor assays, R. oryzae growth was seriously hindered by low concentration of sodium nitrate, high pH value and ozone water treatment. High pH and ozone water affected growth mainly by altering membrane integrity of R. oryzae. Sodium nitrate inhibited the growth of R. oryzae mainly by affecting the amount of sporulation. Low concentration of sodium nitrate and ozone water did not affect the growth of barley. High concentrations of sodium nitrate (100 mM) and pH values (8–8.5) inhibited barley growth. Among them, ozone water had the most obvious inhibition effect on R. oryzae. Large-scale ozone water treatment in the forage grass factory had also played a role in restoring barley production. Taken together, the green techonology to control mold disease and maintain the safety of forage through different physicochemical methods was selected, which was of considerable application value in animal husbandry.
Influence of bundled care treatment on functional outcome in patients with intracerebral hemorrhage
Authors: Mrochen Anne , Song Yu , Harders Verena , Sembill Jochen A. , Sprügel Maximilian I. , Hock Stefan , Lang Stefan , Engelhorn Tobias , Kallmünzer Bernd , Volbers Bastian , Kuramatsu Joji B.
Published: August 2024

Background and aims

General guideline recommendations in patients with intracerebral hemorrhage (ICH) include blood pressure-, temperature- and glucose management. The therapeutic effect of such a “care bundle” (blood pressure lowering, glycemic control, and treatment of pyrexia) on clinical outcomes becomes increasingly established. For the present study, we aimed to investigate associations of strict bundled care treatment (BCT) with clinical outcomes and characterize associations with key outcome effectors such as hematoma enlargement (HE) and peak perihemorrhagic edema (PHE).

Methods

We screened consecutive ICH patients (n = 1,322) from the prospective UKER-ICH cohort study. BCT was defined as achieving and maintaining therapeutic ranges for systolic blood pressure (110–160 mmHg), glucose (80–180 mg/dL), and body temperature (35.5–37.5°C) over the first 72 h. The primary outcome was the functional outcome at 12 months (modified Rankin Scale (mRS) 0–3). Secondary outcomes included mortality at 12 months, the occurrence of hematoma enlargement, and the development of peak perihemorrhagic edema. Confounding was addressed by a doubly robust methodology to calculate the absolute treatment effect (ATE) and by calculating e-values.

Results

A total of 681 patients remained for analysis, and 182 patients fulfilled all three BCT criteria and were compared to 499 controls. The ATE of BCT to achieve the primary outcome was 9.3%, 95% CI (1.7 to 16.9), p < 0.001; e-value: 3.1, CI (1.8). Mortality at 12 months was significantly reduced by BCT [ATE: −12.8%, 95% CI (−19.8 to −5.7), p < 0.001; e-value: 3.8, CI (2.2)], and no association was observed for HE or peak PHE. Significant drivers of BCT effect on the primary outcome were systolic blood pressure control (ATE: 15.9%) and maintenance of normothermia (ATE: 10.9%).

Conclusion

Strict adherence to this “care bundle” over the first 72 h during acute hospital care in patients with ICH was independently associated with improved functional long-term outcome, driven by systolic blood pressure control and maintenance of normothermia. Our findings strongly warrant prospective validation to determine the generalizability especially in Western countries.

Clinical trial registration:ClinicalTrials.gov, identifier [ID: NCT03183167].
Bidirectional two-sample Mendelian randomization analysis identifies causal associations between migraine and five psychiatric disorders
Authors: Li Wen-Wei , Zhang Jia-Xin , Wang Jia , Chen Ya-qing , Lai Sha , Qiu Zhi-Kun
Published: August 2024

Background

The question of whether a correlation exists between migraine and five psychiatric disorders, including posttraumatic stress disorder (PTSD), major depressive disorder (MDD), anorexia nervosa (AN), bipolar disorder (BIP), and schizophrenia (SCZ), remains a matter of controversy. Hence, this research aims to investigate whether there is a possible association between migraine and five psychiatric disorders.

Methods

We performed a bidirectional 2-sample Mendelian randomization (MR) analysis to assess the causality between migraine and five psychiatric disorders. Genetic associations of PTSD, MDD, AN, BIP, and SCZ were obtained from the Psychiatric Genomics Consortium (PGC) database and genetic associations of migraine with aura and migraine without aura were obtained from the FinnGen dataset. We used the inverse-variance weighted (IVW), weighted median, weighted mode, MR Pleiotropy RESidual Sum and Outlier (MR-PRESSO), and MR Egger regression methods to evaluate the association of genetically predicted exposure with the risk of outcome.

Results

MR demonstrated that MDD was associated with a high risk of migraine without aura (OR = 1.930578, 95% confidence interview (CI): 1.224510, 3.043550, p < 0.05), but BIP was related to a low risk of migraine without aura (OR = 0.758650, 95%CI: 0.639601, 0.899858, p < 0.05). According to the results of reverse MR, migraine with aura was associated with a high risk of BIP (OR = 1.019100, 95%CI: 1.002538, 1.035935, p < 0.05), and migraine without aura was associated with an increased risk of AN (OR = 1.055634, 95%CI: 1.023859, 1.088394, p < 0.05).

Conclusion

Our results provide evidence of the potential causal association between migraine and some psychiatric disorders. It may contribute to the prevention of migraine and some psychiatric disorders.
Tracing the evolving dynamics and research hotspots of spinal cord injury and surgical decompression from 1975 to 2024: a bibliometric analysis
Authors: Wang Siqiao , Xu Wei , Wang Jianjie , Hu Xiao , Wu Zhourui , Li Chen , Xiao Zhihui , Ma Bei , Cheng Liming
Published: August 2024

Background

Exploration of the benefits and timing of surgical decompression in spinal cord injury (SCI) has been a research hotspot. However, despite the higher volume and increasing emphasis on quality there remains no bibliometric view on SCI and surgical decompression. In this study, we aimed to perform bibliometric analysis to reveal the core countries, affiliations, journals, authors, and developmental trends in SCI and surgical decompression across the past 50 years.

Methods

Articles and reviews were retrieved from web of science core collection between 1975 and 2024. The bibliometrix package in R was used for data analysis and visualizing.

Results

A total of 8,688 documents were investigated, indicating an ascending trend in annual publications. The USA and China played as the leaders in scientific productivity. The University of Toronto led in institutional productions. Core authors, such as Michael G. Fehlings, showed high productivity, and occasional authors showed widespread interests. Core journals like Spine and Spinal Cord served as beacons in this field. The interaction of core authors and international collaboration accentuated the cross-disciplinary feature of the field. Prominent documents emphasized the clinical significance of early decompression in 24 h post SCI.

Conclusion

Based on comprehensive bibliometric analysis and literature review, we identified the hotspots and future directions of this field: (1) further investigation into the molecular and cellular mechanisms to provide pre-clinical evidence for biological effects of early surgical decompression in SCI animal models; (2) further evaluation and validation of the optimal time window of surgical decompression based on large cohort, considering the inherent heterogeneity of subpopulations in complicated immune responses post SCI; (3) further exploration on the benefits of early decompression on the neurological, functional, and clinical outcomes in acute SCI; (4) evaluation of the optimal surgical methods and related outcomes; (5) applications of artificial intelligence-based technologies in spinal surgical decompression.
The use of matrine to inhibit osteosarcoma cell proliferation via the regulation of the MAPK/ERK signaling pathway
Authors: Huang Xincheng , Zeng Jun , Ruan Siyuan , Lei Zhuolin , Zhang Jingyuan , Cao Hong
Published: August 2024

Background

Matrine is an alkaloid extracted from Sophorus beans of the legume family, and it has significant effects and a variety of pharmacological activities. Osteosarcoma(OS) is a common malignant bone tumor that is characterized by high incidence and rapid progression. There have been some preliminary studies on the therapeutic effect of matrine on OS, but the specific mechanism remains unclear.

Objective

The aim of this study was to investigate the antitumor effect of matrine on HOS cells and the underlying molecular mechanism.

Methods

The effects of matrine on the proliferation, apoptosis and cell cycle progression of HOS cells were determined by CCK-8 assay, TUNEL assay and flow cytometry in vitro. Wound healing and Transwell invasion assays were used to observe the effect of matrine on the migration and invasion of HOS cells. The mechanism underlying the antitumor effect of matrine on HOS cells was investigated by Western blotting.

Results

Matrine significantly inhibited HOS cell proliferation, promoted HOS cell apoptosis, and arrested HOS cells in the G1 phase of the cell cycle. Both wound healing and Transwell invasion assays showed that matrine inhibited HOS cell migration and invasion. Western blotting results showed that matrine inhibited the activation of the MAPK/ERK signaling pathway. We found that matrine also downregulated Bcl-2 expression, which may be related to protein synthesis inhibition.

Conclusion

Matrine can inhibit the proliferation of HOS cells, arrest HOS cells in the G1 phase, and promote HOS cell apoptosis through the MAPK/ERK signaling pathway.
Epigenetic regulation of NKG2D ligand and the rise of NK cell-based immunotherapy for cancer treatment
Authors: Kumar Raj , Gupta Romi
Published: August 2024

Epigenetic modifications influence gene expression and effects cancer initiation and progression. Therefore, they serve as diagnostic and prognostic biomarkers and potential therapeutic targets. Natural Killer (NK) cells, integral to the innate immune system, exhibit anti-tumor effect by recognizing and eliminating cancerous cells through the balance of activating and inhibitory ligands. Understanding the epigenetic regulation of NK cell ligands offers insights into enhancing NK cell-mediated tumor eradication. This review explores the epigenetic modifications governing the expression of activating NKG2D ligands and discusses clinical trials investigating NK cell-based immunotherapies, highlighting their potential as effective cancer treatment strategies. Case studies examining the safety and effectiveness of NK cell therapies in different cancer types, such as acute myeloid leukemia (AML) and non-small cell lung cancer (NSCLC), demonstrate promising outcomes with minimal toxicity. These findings underscore the therapeutic prospects of epigenetic modulation of NKG2D ligands and NK cell-based immunotherapies as effective cancer treatment strategies. Future research in the advancement of personalized medicine approaches and novel combination therapies with NK cell will further improve treatment outcomes and provide new therapeutic options for treating patients with various types of cancer.
Risk of low levels of blood group antibodies mediating hemolysis in ABO-incompatible neonates with negative three hemolysis tests
Authors: Lin Hongxing , Luo Pingxiang , Liu Chen , Lin Xiaosong , Que Chengwen , Zhong Wenhui
Published: August 2024

Objective

To explore the risk of low-level blood group antibody-mediated hemolysis in ABO-incompatible newborns with negative three hemolysis tests, aiming to assist in the identification and management of neonatal jaundice.

Methods

A retrospective case-control study was performed in 892 children with jaundice. The patients were divided into three groups: group I, ABO compatible, negative three hemolysis tests; group II, ABO incompatible, negative three hemolysis tests; and group III, ABO incompatible, positive three hemolysis tests. We analyzed the differences in clinical data, blood routine and biochemical laboratory results.

Results

(1) Patients in group II had higher levels of mean corpuscular volume (MCV), standard deviation of red blood cell volume distribution width (RDW-SD), alanine aminotransferase (ALT), lactate dehydrogenase (LDH), alkaline phosphatase (ALP), and bile acid (BA) than those in group I (P < 0.05). However, there were no statistically significant differences in the MCV, ALT, ALP and BA levels between groups II and III (P > 0.05). (2) Mean corpuscular hemoglobin concentration (MCHC) >359.5 g/L, cell volume distribution width (RDW-CV) >15.95%, and reticulocyte count (RET) >4.235% were identified as independent predictors of positive hemolysis test results (P < 0.001). The combination of MCHC, RDW-CV, and RET% yielded an AUC of 0.841.

Conclusion

Low-level blood group antibody-mediated hemolysis may occur in ABO-incompatible neonates even when three hemolysis tests are negative. Changes in liver function parameters must be monitored. The combination of MCHC, RDW-CV, and RET% can be used to improve the detection rate of HDN.
Chitosan-based biomaterial delivery strategies for hepatocellular carcinoma
Authors: Wang Xianling , Yang Yan , Zhao Shuang , Wu Di , Li Le , Zhao Zhifeng
Published: August 2024

Background

Hepatocellular carcinoma accounts for 80% of primary liver cancers, is the most common primary liver malignancy. Hepatocellular carcinoma is the third leading cause of tumor-related deaths worldwide, with a 5-year survival rate of approximately 18%. Chemotherapy, although commonly used for hepatocellular carcinoma treatment, is limited by systemic toxicity and drug resistance. Improving targeted delivery of chemotherapy drugs to tumor cells without causing systemic side effects is a current research focus. Chitosan, a biopolymer derived from chitin, possesses good biocompatibility and biodegradability, making it suitable for drug delivery. Enhanced chitosan formulations retain the anti-tumor properties while improving stability. Chitosan-based biomaterials promote hepatocellular carcinoma apoptosis, exhibit antioxidant and anti-inflammatory effects, inhibit tumor angiogenesis, and improve extracellular matrix remodeling for enhanced anti-tumor therapy.

Methods

We summarized published experimental papers by querying them.

Results and Conclusions

This review discusses the physicochemical properties of chitosan, its application in hepatocellular carcinoma treatment, and the challenges faced by chitosan-based biomaterials.
Lensless multi-spectral holographic interferometry for optical inspection
Authors: Agour Mostafa , Thiemicke Fabian , Müller André F. , Bergmann Ralf B. , Falldorf Claas
Published: August 2024

We explore the principles, implementation details, and performance characteristics of a lensless multi-spectral digital holographic sensor and demonstrate its potential for quality assurance in semiconductor manufacturing. The method is based on capturing multi-spectral digital holograms, which are subsequently utilized to evaluate the shape of a reflective test object. It allows for a compact setup satisfying high demands regarding robustness against mechanical vibrations and thus overcomes limitations associated with conventional optical inspection setups associated with lens-based white light interferometry. Additionally, the tunable laser source enhances the versatility of the system and enables adaptation to various sample characteristics. Experimental results based on a wafer test specimen demonstrate the effectiveness of the method. The axial resolution of the sensor is ±2.5 nm, corresponding to 1σ.
Artificial light at night decreases leaf herbivory in typical urban areas
Authors: Cao Yu , Zhang Shuang , Ma Ke-Ming
Published: August 2024

Artificial light at night (ALAN) is exerting growing pressure on natural ecosystems, but its impact on biological interactions remains unclear. This study aimed to assess how ALAN influences leaf functional traits and herbivory in two prevalent street tree species (Styphnolobium japonicum (L.) Schott and Fraxinus pennsylvanica) through field surveys and paired experiments in the urban areas of Beijing, China. We found that ALAN led to increased leaf toughness and decreased levels of leaf herbivory. Additionally, ALAN showed species-specific effects on leaf nutrients, size as well as defense substances. The findings illustrate that ALAN can significantly alter some key functional traits and ecological processes (nutrient cycling, energy flow). In general, we suggest that high ALAN intensity will be detrimental to the energy flow from urban plants to higher trophic levels, posing a potential threat to the maintenance of biodiversity (e.g., arthropod diversity, bird diversity) in urban ecosystems.
Ectopic overexpression of ShCBF1 and SlCBF1 in tomato suggests an alternative view of fruit responses to chilling stress postharvest
Authors: Albornoz Karin , Zhou Jiaqi , Zakharov Florence , Grove Jonas , Wang Minmin , Beckles Diane M.
Published: August 2024

Postharvest chilling injury (PCI) is a physiological disorder that often impairs tomato fruit ripening; this reduces fruit quality and shelf-life, and even accelerates spoilage at low temperatures. The CBF gene family confers cold tolerance in Arabidopsis thaliana, and constitutive overexpression of CBF in tomato increases vegetative chilling tolerance, in part by retarding growth, but, whether CBF increases PCI tolerance in fruit is unknown. We hypothesized that CBF1 overexpression (OE) would be induced in the cold and increase resistance to PCI. We induced high levels of CBF1 in fruit undergoing postharvest chilling by cloning it from S. lycopersicum and S. habrochaites, using the stress-inducible RD29A promoter. Harvested fruit were cold-stored (2.5°C) for up to three weeks, then rewarmed at 20°C for three days. Transgene upregulation was triggered during cold storage from 8.6- to 28.6-fold in SlCBF1-OE, and between 3.1- to 8.3-fold in ShCBF1-OE fruit, but developmental abnormalities in the absence of cold induction were visible. Remarkably, transgenic fruit displayed worsening of PCI symptoms, i.e., failure to ripen after rewarming, comparatively higher susceptibility to decay relative to wild-type (WT) fruit, lower total soluble solids, and the accumulation of volatile compounds responsible for off-odors. These symptoms correlated with CBF1 overexpression levels. Transcriptomic analysis revealed that the ripening and biotic and abiotic stress responses were altered in the cold-stored transgenic fruit. Seedlings grown from ‘chilled’ and ‘non-chilled’ WT fruit, in addition to ‘non-chilled’ transgenic fruit were also exposed to 0°C to test their photosynthetic response to chilling injury. Chilled WT seedlings adjusted their photosynthetic rates to reduce oxidative damage; ‘non-chilled’ WT seedlings did not. Photosynthetic parameters between transgenic seedlings were similar at 0°C, but SlCBF1-OE showed more severe photoinhibition than ShCBF1-OE, mirroring phenotypic observations. These results suggest that 1) CBF1 overexpression accelerated fruit deterioration in response to cold storage, and 2) Chilling acclimation in fructus can increase chilling tolerance in seedling progeny of WT tomato.
Guessing errors made by children with dyslexia in word and text reading
Authors: De Rom Margot , Van Reybroeck Marie
Published: August 2024

Introduction

Children with dyslexia face persistent difficulties in reading acquisition, which results in poor reading accuracy. In addition to the commonly studied reading errors such as omissions, additions, substitutions, and letter reversals, they also make guessing errors characterized by replacing a word with an orthographic neighbor. These errors, which occur in the context of isolated words and sentence or text reading, might be linked to the inhibition issues that have already been demonstrated in connection to dyslexia. However, to the best of our knowledge, there is no experimental evidence that children with dyslexia make more guessing errors than typically developing children, as is the case for sequential errors. The current study aimed to evaluate whether children with dyslexia made more guessing errors than typically developing children and whether these errors were more frequent in an isolated word or sentence context.

Methods

Twenty-eight children with dyslexia from Grade 4 were matched with typically developing children by either chronological age or reading level. Reading was assessed through word and text reading tasks. Error types were classified into seven categories and analyzed.

Results and conclusion

A repeated-measure ANOVA showed that children with dyslexia made more guessing errors, particularly visual and morphemic errors, than typically developing children. Moreover, these errors were found at both the single word and sentence levels. These findings suggest that children with dyslexia tend to use a global treatment of words, relying on incomplete information to compensate for their difficulties. The findings have practical implications for pedagogical and therapeutic approaches.
Assessing family relationships through drawing: the Family Life Space
Authors: Gennari Marialuisa , Gozzoli Caterina F. , Tamanza Giancarlo
Published: August 2024

The Family Life Space (FLS) is a graphic instrument that may aid the relational assessment of families. This interactive instrument involves all members of the family in a joint task, that of collectively making a drawing of their own family system and it allows the gathering of information related to the overall family organization. The FLS was originally conceived by Danuta Mostwin in the early 70s and used as an instrument for clinical intervention. After having been applied to several contexts, the present contribution aims at presenting the key indicators to use the instrument as a tool for assessing family relations. Specifically, the characteristics of the instrument that allow the gathering of relevant information at the individual, relational, and family level will be outlined. For each of these levels, the data regarding the quantity and quality of the elements in the drawing that define the family space (i.e., the number and graphic quality of the actual elements in the drawing) as well the quantity and quality of the relationships among family members and with their community at large (i.e., the number and type of lines connecting the various elements in the drawing) will be presented. The instrument can therefore provide useful insights on the following constructs: quality of life, power dynamics within the family, feelings of belonging, closeness and/or distance as well as conflict or acknowledgement between family members and the overall attitude family members have toward their context and the critical events they had to face. The application and complete potential of the instrument are further elaborated upon through the presentation of a clinical case. This case not only aids in comprehending the tool’s usage but also enables the collection of psychological information about the family and provides a clinical interpretation of family relationships.
Consciousness and education: contributions by Piaget, Vygotsky and Steiner
Authors: Stoltz Tania , Weger Ulrich , da Veiga Marcelo
Published: August 2024

The objective of this study is to gain an understanding of the development of consciousness and its relationship with education based on different theoretical models—namely those by Piaget, Vygotsky and Steiner. All three of them focus on different subcomponents of the educational process and there is hence a need for integrative discussion. Piaget and Vygotsky are fundamental references in the understanding of developmental and learning processes. Steiner was a pioneer in proposing a pedagogy that progresses by integrating feeling and wanting alongside thinking in the educational process. Their theories have important similarities but also differences and these will be essential for broadening the understanding of the construction of consciousness and its relationships with education.
Systemic immune inflammation index is a valuable marker for predicting hemodialysis patients with depression: a cross-sectional study
Authors: Han Xi-xi , Zhang Hui-ying , Kong Jing-wen , Liu Yu-xin , Zhang Ke-ren , Ren Wen-ying
Published: August 2024

Objective

Maintenance hemodialysis (MHD) patients suffer from enormous physical, mental stress and poor quality of life, so an increasing number of patients are in a long-term state of depression. A prominent feature of MHD patients is chronic persistent inflammation, which is also an important mechanism for the onset of depression. Therefore, finding economically convenient inflammatory markers to predict and diagnose the onset of depression in MHD patients is of great value. As a novel inflammatory marker, systemic immune inflammation index (SII) can more comprehensively reflect the inflammation and immunity level of patients. This study aims to explore the relationship between SII and depressive symptoms in MHD patients.

Methods

A cross-sectional study was conducted on 206 MHD patients from three dialysis centers. Based on the Hospital Anxiety and Depression Scale (HADS) scores, patients were divided into non-depression and depression groups. Inter group comparison and multivariate logistic regression analysis were performed to determine whether SII is an independent risk factor for depression in MHD patients. Receiver operating characteristic (ROC) curve was used to evaluate the predictive value of SII on depression symptoms in MHD patients.

Results

According to the HADS scale score, 38.83% of the included patients were in a state of depression. After adjusting for all confounding factors, MHD patients with SII>963.93 had a 4.709 times higher risk of depression than those with SII ≤ 478.32 (OR=4.709, 95% CI 1.821–12.178, P<0.01). ROC analysis showed that SII>685.11 was the best cutoff value for MHD depression patients, and the area under the curve (AUC) was 0.681.

Conclusions

High SII is an independent risk factor for depressed MHD patients and an ideal inflammatory marker for predicting and identifying depression in MHD patients as assessed by the HADS scale.
The bench to community initiative: community-based participatory research model for translating research discoveries into community solutions
Authors: Tapia Jazma L. , Lopez Abigail , Turner D. Bing , Fairley Tonya , Tomlin-Harris Tiah , Hawkins Maggie , Holbert Pastor Rhonda , Treviño Lindsey S. , Teteh-Brooks Dede K.
Published: August 2024

Community-based participatory research (CBPR) is an effective methodology for translating research findings from academia to community interventions. The Bench to Community Initiative (BCI), a CBPR program, builds on prior research to engage stakeholders across multiple disciplines with the goal of disseminating interventions to reduce breast cancer disparities and improve quality of life of Black communities.

Methods

The BCI program was established to understand sociocultural determinants of personal care product use, evaluate the biological impact of endocrine disrupting chemicals, and develop community interventions. The three pillars of the program include research, outreach and engagement as well as advocacy activities. The research pillar of the BCI includes development of multidisciplinary partnerships to understand the sociocultural and biological determinants of harmful chemical (e.g., endocrine disrupting chemicals) exposures from personal care products and to implement community interventions. The outreach and engagement pillar includes education and translation of research into behavioral practice. The research conducted through the initiative provides the foundation for advocacy engagement with applicable community-based organizations. Essential to the mission of the BCI is the participation of community members and trainees from underrepresented backgrounds who are affected by breast cancer disparities.

Results

Two behavioral interventions will be developed building on prior research on environmental exposures with the focus on personal care products including findings from the BCI. In person and virtual education activities include tabling at community events with do-it-yourself product demonstrations, Salon Conversations—a virtual platform used to bring awareness, education, and pilot behavior change interventions, biennial symposiums, and social media engagement. BCI’s community advisory board members support activities across the three pillars, while trainees participate in personal and professional activities that enhance their skills in research translation.

Discussion

This paper highlights the three pillars of the BCI, lessons learned, testimonies from community advisory board members and trainees on the impact of the initiative, as well as BCI’s mission driven approaches to achieving health equity.
Understanding the cataract treatment disparities among older adults in India
Authors: Singh Rajeev Ranjan , Mohanty Sanjay K.
Published: August 2024

Background

Cataract is a leading cause of global blindness, affecting around 33% of blind individuals worldwide. It significantly impacts individuals’ well-being, independence, and quality of life, posing a substantial economic burden. India’s rapidly ageing population necessitates an examination of cataract prevalence and treatment disparities. No attempts have been made to address socioeconomic variation in treatment disparities of effective cataract treatment coverage among older adults in India.

Data and method

This study utilises data from the Longitudinal Ageing Study of India (LASI) conducted in 2017–18, that covered, 73,396 individuals aged 45 and above. Logistic regression, univariate, and bivariate analyses were employed to understand the variation of cataract and their associations with various demographic factors. Visual acuity tests and self-reported cataract data were used.

Results

The prevalence of cataract among older adults in India was 14.25%, with higher rates among females and the older adult. Socioeconomic disparities werelarge, with lower prevalence among those with higher education and urban residence. Despite the effectiveness of cataract surgery, disparities in treatment access and effective coverage persisted. Approximately 27.52% of older adults did not receive cataract treatment, and those who received out of them 28% did not receive effective treatment. The effective treatment was lower among female, less educated, and poor.

Conclusion

Cataract remains a significant public health concern in India, particularly among older adults. The study highlights the importance of addressing socioeconomic disparities in cataract treatment access and quality of care. Targeted interventions are needed to bridge these gaps, ultimately improving visual health outcomes and well-being among older adults in India.