bluuebunny/crossref_metadata_2025 huggingface dataset:
G870A Polymorphic Variants of CCND1 Gene and Cyclin D1 Protein Expression as Prognostic Markers in Laryngeal Lesions
Authors: Kowalczyk Magdalena Marianna , Barańska Magda , Fendler Wojciech , Borkowska Edyta M. , Kobos Józef , Borowiec Maciej , Pietruszewska Wioletta
Published: April 2022

CCND1 gene encodes Cyclin D1 protein, the alternations and overexpression of which are commonly observed in human cancers. Cyclin D1 controls G1-S transition in the cell cycle. The aim of the study was to assess utility of the genotyping and protein expression in predicting the susceptibility of transformation from normal tissue to precancerous laryngeal lesions (PLLs) and finally to laryngeal cancer (LC). Four hundred and thirty-five patients (101 with LC, 100 with PLLs and 234 healthy volunteers) were enrolled in the study. Cyclin D1 expression was examined by immunohistochemistry and G870A polymorphism of gene CCND1 by PCR-RFLP technique. We confirmed association between the A allele and risk of developing LC from healthy mucosa (p = 0.006). Significantly higher expression of Cyclin D1 was observed in LC compering with PLLs (p < 0.0001) and we found that it could be a predictive marker of shorter survival time. To sum up, in the study population CCND1 gene polymorphism A870G and Cyclin D1 expression have a significant impact on the risk of developing PLLs and LC, and, therefore, Cyclin D1 could be a useful marker for the prediction of survival time in LC, whereas CCND1 gene polymorphism does not have a direct impact on patients’ outcome.
Lower Macromolecular Content in Tendons of Female Patients with Osteoporosis versus Patients with Osteopenia Detected by Ultrashort Echo Time (UTE) MRI
Authors: Jerban Saeed , Ma Yajun , Afsahi Amir Masoud , Lombardi Alecio , Wei Zhao , Shen Meghan , Wu Mei , Le Nicole , Chang Douglas G. , Chung Christine B. , Du Jiang , Chang Eric Y.
Published: April 2022

Tendons and bones comprise a special interacting unit where mechanical, biochemical, and metabolic interplays are continuously in effect. Bone loss in osteoporosis (OPo) and its earlier stage disease, osteopenia (OPe), may be coupled with a reduction in tendon quality. Noninvasive means for quantitatively evaluating tendon quality during disease progression may be critically important for the improvement of characterization and treatment optimization in patients with bone mineral density disorders. Though clinical magnetic resonance imaging (MRI) sequences are not typically capable of directly visualizing tendons, ultrashort echo time MRI (UTE-MRI) is able to acquire a high signal from tendons. Magnetization transfer (MT) modeling combined with UTE-MRI (i.e., UTE-MT-modeling) can indirectly assess macromolecular proton content in tendons. This study aimed to determine whether UTE-MT-modeling could detect differences in tendon quality across a spectrum of bone health. The lower legs of 14 OPe (72 ± 6 years) and 31 OPo (73 ± 6 years) female patients, as well as 30 female participants with normal bone (Normal-Bone, 36 ± 19 years), are imaged using UTE sequences on a 3T MRI scanner. Institutional review board approval is obtained for the study, and all recruited subjects provided written informed consent. A T1 measurement and UTE-MT-modeling are performed on the anterior tibialis tendon (ATT), posterior tibialis tendon (PTT), and the proximal Achilles tendon (PAT) of all subjects. The macromolecular fraction (MMF) is estimated as the main measure from UTE-MT-modeling. The mean MMF in all the investigated tendons was significantly lower in OPo patients compared with the Normal-Bone cohort (mean difference of 24.2%, p < 0.01), with the largest Normal-Bone vs. OPo difference observed in the ATT (mean difference of 32.1%, p < 0.01). Average MMF values of all the studied tendons are significantly lower in the OPo cohort compared with the OPe cohort (mean difference 16.8%, p = 0.02). Only the PPT shows significantly higher T1 values in OPo patients compared with the Normal-Bone cohort (mean difference 17.6%, p < 0.01). Considering the differences between OPo and OPe groups with similar age ranges, tendon deterioration associated with declining bone health was found to be larger than a priori detected differences caused purely by aging, highlighting UTE-MT MRI techniques as useful methods in assessing tendon quality over the course of progressive bone weakening.
Prognostic Value of Perineural Invasion on Survival and Recurrence in Oral Squamous Cell Carcinoma
Authors: Spoerl Steffen , Spoerl Silvia , Reil Stephanie , Gerken Michael , Ludwig Nils , Taxis Juergen , Fischer René , Ettl Tobias , Reichert Torsten E. , Spanier Gerrit
Published: April 2022

A diagnosis of perineural invasion (PNI) is widely accepted as an unfavorable prognostic factor in various solid malignancies. Although PNI has been described as a high-risk parameter in oral squamous cell carcinoma (OSCC), its role in the current staging manuals of the American Joint Committee on Cancer (AJCC) is rather subordinate. We analysed the prognostic value of PNI on survival and recurrence in a large, multicenter OSCC cohort and a population-based approach. A total of 493 OSCC patients with primary tumor resection to negative margins and concomitant neck dissection between 2010 and 2017 were enrolled. PNI was evaluated in relation to overall survival (OAS) and recurrence-free survival (RFS) using uni- and multi-variable Cox regression. The median follow-up time was 5.0 years and PNI was diagnosed in 48 patients (9.7%). A pathohistological verification of PNI correlated significantly with a deteriorated OAS in uni- (HR 2.312; 95% CI 2.312–3.493, p = 0.001) and multivariable Cox regression (HR 1.820; 95% CI 1.164–2.847, p = 0.009). Additionally, a diagnosis of PNI correlated with increased cumulative, as well as distant, metastasis 5-year-recurrence rates (p = 0.027 and p = 0.011, respectively). The application of adjuvant radiotherapy (RT) or radiochemotherapy (RCT) in patients with PNI did not alter OAS or RFS in survival analysis when compared to patients without PNI. The results underline the adverse impact of PNI on the survival and recurrence of surgically treated OSCC patients. Based on our findings, we highly recommend an emphasis on PNI in the TNM staging concept.
Electrified Powertrains for a Sustainable Mobility: Topologies, Design and Integrated Energy Management Strategies
Authors: Tribioli Laura , Villani Manfredi
Published: April 2022

The Special Issue “Electrified Powertrains for a Sustainable Mobility: Topologies, Design and Integrated Energy Management Strategies” has been proposed with the main objective of contributing to the sustainable mobility agenda through enhanced scientific and multi-disciplinary topics, aimed at addressing concerns and real possibilities in the achievement of a greener mobility [...]
A Rolling Horizon Optimization Framework for Resilient Restoration of Active Distribution Systems
Authors: Xin Ning , Chen Laijun , Ma Linrui , Si Yang
Published: April 2022

Network reconfiguration is an effective way to avoid severe, large-scale power outages and to improve the resilience of active distribution networks (ADNs). Furthermore, the rapid development of distributed energy resources (DERs) provides new perspectives for network reconfiguration. In this paper, the effect of network reconfiguration and DER collaboration on ADN’s resilient restoration are studied. The applications of DERs are fully explored. In order to achieve a better resilient performance, a detailed multiperiod model considering both reconfiguration and multiple DERs is established. Some linearization techniques are used to simplify the proposed model. Then, we build a rolling horizon optimization framework to solve the model. The framework eliminates the adverse effect of prediction errors and speeds up the calculation. By introducing predictions into strategy determination, the framework achieves a better restoration effect than the traditional greedy method. The proposed framework is tested on a 33-bus system. The simulations verify the efficiency of our work.
Optimization Design of Unequal Amplitude Modulated Poles for the Bearingless PMSM
Authors: Wang Huimin , Lu Yuting , Wu Shuang , Guo Liyan
Published: April 2022

The structural parameters of an equal amplitude modulated magnetic pole is limited by the length of the air-gap. When the modulation ratio or carrier ratio is small, the spacing of permanent magnets is too large, which will lead to a worse cogging effect, and then the torque optimization effect is not satisfying. In order to improve the operation stability of a bearingless permanent magnet synchronous motor (bearingless PMSM), an unequal amplitude modulated magnetic pole structure is proposed according to the principle of magnetic pole modulation. The Taguchi method is used to optimize the structural parameters of the unequal amplitude modulated magnetic pole with the goal of reducing the torque and suspension force fluctuation. Three kinds of magnetic pole structures, named the whole magnetic pole, the equal amplitude modulated magnetic pole and the unequal amplitude modulated magnetic pole are compared and verified. The results show that the proposed structure of the unequal amplitude modulated magnetic pole can effectively further decrease the torque and suspension force fluctuations under the premise of the output capacity of the motor being unchanged, which improves the performance of the bearingless PMSM.
Seed Dispersal Models for Natural Regeneration: A Review and Prospects
Authors: Kim Moonil , Lee Seonghun , Lee Songhee , Yi Koong , Kim Hyung-Sub , Chung Sanghoon , Chung Junmo , Kim Hyun Seop , Yoon Tae Kyung
Published: April 2022

Natural regeneration in forest management, which relies on artificial planting, is considered a desirable alternative to reforestation. However, there are large uncertainties regarding the natural regeneration processes, such as seed production, seed dispersal, and seedling establishment. Among these processes, seed dispersal by wind must be modeled accurately to minimize the risks of natural regeneration. This study aimed to (1) review the main mechanisms of seed dispersal models, their characteristics, and their applications and (2) suggest prospects for seed dispersal models to increase the predictability of natural regeneration. With improving computing and observation systems, the modeling technique for seed dispersal by wind has continued to progress steadily from a simple empirical model to the Eulerian-Lagrangian model. Mechanistic modeling approaches with a dispersal kernel have been widely used and have attempted to be directly incorporated into spatial models. Despite the rapid development of various wind-dispersal models, only a few studies have considered their application in natural regeneration. We identified the potential attributes of seed dispersal modeling that cause high uncertainties and poor simulation results in natural regeneration scenarios: topography, pre-processing of wind data, and various inherent complexities in seed dispersal processes. We suggest that seed dispersal models can be further improved by incorporating (1) seed abscission mechanisms by wind, (2) spatiotemporally complex wind environments, (3) collisions with the canopy or ground during seed flight, and (4) secondary dispersal, long-distance dispersal, and seed predation. Interdisciplinary research linking climatology, biophysics, and forestry would help improve the prediction of seed dispersal and its impact on natural regeneration.
Effects of Sucrose, Glucose and Molasses on Fermentation Quality and Bacterial Community of Stylo Silage
Authors: Zi Xuejuan , Liu Yue , Chen Ting , Li Mao , Zhou Hanlin , Tang Jun
Published: April 2022

To better understand the mechanism underlying water-soluble carbohydrates (WSC)-regulated silage fermentation, the bacterial community and fermentation quality of stylo (Stylosanthes guianensis) ensiled without (sterile distilled water) (CK) or with 10 g/kg sucrose (S), 10 g/kg glucose (G), and 10 g/kg molasses (M) were investigated. Each treatment was ensiled in three plastic bag silos for 30 days. The DM of stylo was 301.0 g/kg (fresh matter basis), while the contents of CP, WSC, NDF and ADF were 104.9, 12.3, 510.4 and 407.6 g/kg (dry matter basis), respectively. The bacterial community was characterized by using 16Sr DNA sequencing technology. The addition of WSC significantly altered both fermentation quality and the bacterial community of stylo silage. The additive treatment resulted in higher lactic acid (17.2 vs. 67.0 g/kgDM), lower pH (4.68 vs. 4.46), and lower acetic acid (37.0 vs. 28.9 g/kgDM) compared with the control. In addition, no propionic acid and butyric acid were detected in the additive treatment groups. These results indicated that WSC additives helped to produce greater quality stylo silage. Meanwhile, the Shannon index was higher, and the Simpson index was lower in the WSC additive-treated groups compared with the control, indicating that WSC improved microbial diversity. Furthermore, WSC treatments increased the abundance of acid-producing bacteria Megamonas and Bacteroides, decreased the abundance of Weissella, and inhibited the growth of the undesirable Enterobacter. Our results confirmed that sucrose, glucose and molasses have similar beneficial effects on both bacterial community and silage fermentation of stylo. Molasses was recommended to be used in stylo silage for economic benefit and resource utilization.
In Vitro Anti-Obesity Effect of Shenheling Extract (SHLE) Fermented with Lactobacillus fermentum grx08
Authors: Yan Xian-Tao , Zhang Wenmiao , Zhang Yanyan , Zhang Ziqi , Chen Dawei , Wang Wenqiong , Ma Wenlong , Qu Hengxian , Qian Jian-Ya , Gu Ruixia
Published: April 2022

Obesity is a common global problem. There are many fat-reducing herbal prescriptions in traditional Chinese medicine that have been proven to be safe and functional during long-term application. Microbial fermentation can improve the efficacy of herbal medicine and improve the unsavory flavor. In this study, Shenheling extract (SHLE) composed of six medicine food homology materials was used as the research object. The purpose of this study was to evaluate the effects of Lactobacillusfermentum grx08 fermentation on the antiobesity efficacy and flavor of SHLE. We found that L. fermentum grx08 grew well in SHLE. After 72 h of fermentation, the total polysaccharides, total flavonoids, total polyphenols and total saponins of SHLE decreased, but the lipase inhibitory activity and total antioxidant capacity (FRAP) were significantly increased (p < 0.01). There were no significant differences in the α-glucosidase inhibition rate and DPPH· clearance rate before or after fermentation (p > 0.05). In addition, the fermentation reduces the unpleasant flavors of SHLE such as bitterness and grassy and cassia flavors. This study demonstrates that SHLE fermented by L. fermentum grx08 improved some anti-obesity functions and improved the unpleasant flavor.
Progressive Unspecified Motor Speech Disorder: A Longitudinal Single Case Study of an Older Subject
Authors: Basagni Benedetta , Martelli Sonia , Ruffini Livia , Mazzucchi Anna , Cecchi Francesca
Published: April 2022

Introduction: In a few cases, neurodegenerative diseases debut with a speech disorder whose differential diagnosis can be difficult. Case Report: We describe the case of a right-handed woman with a progressive speech impairment, which debuted when she was 80 years old. We report the results of neurological, neuropsychological, and imaging assessments with positron emission tomography (PET) over a period of nine years. Metabolic PET with 18F-FDG was performed at the age of 81 and repeated two years later due to the worsening of symptoms; amyloid PET with 18F-flutemetamol was performed at the age of 86. All PET results were quantitatively analyzed. A speech impairment remained the isolated neurological symptom for a long time, together with a mood disorder. Early FDG-PET showed hypometabolism in the left superior and inferior frontal areas, in the left superior temporal area, and in the right superior frontal area. Two years later, the hypometabolic area was more extensive. Amyloid PET was qualitatively and quantitatively normal. Nine years after the first symptoms, the speech production progressively worsened until complete anarthria, in association with writing impairment onset and signs of behavioral impairments. No signs of motor involvement were found. Conclusions: A progressive articulatory disorder without an evolution of motor disorders may be a distinct neurological degenerative entity, mainly affecting speech production for very a long time and with a specific early metabolic pattern in brain FDG-PET in the language production area. Monitoring patients with FDG-PET could predict the disease evolution years before a clinical deterioration.
Stateful-Service-Based Pupil Recognition in Natural Light Environments
Authors: Ke Rih-Shen , Horng Gwo-Jiun , Chen Kuo-Tai , Lee Kuo-Chang
Published: April 2022

Smartphones are currently extensively used worldwide, and advances in hardware quality have engendered improvements in smartphone image quality, which is occasionally comparable to the quality of medical imaging systems. This paper proposes two algorithms for pupil recognition: a stateful-service-based pupil recognition mechanism and color component low-pass filtering algorithm. The PRSSM algorithm can determine pupil diameters in images captured in indoor natural light environments, and the CCLPF algorithm can determine pupil diameters in those captured outdoors under sunlight. The PRSSM algorithm converts RGB colors into the hue saturation value color space and performs adaptive thresholding, morphological operations, and contour detection for effectively analyzing the diameter of the pupil. The CCLPF algorithm derives the average matrix for the red components of eye images captured in outdoor environments. It also performs low-pass filtering, morphological and contour detection operations, and rule-of-thumb correction. This algorithm can effectively analyze pupil diameter in outdoor natural light. Traditional ruler-based measurements of pupil diameter were used as the reference to verify the accuracy of the PRSSM and CCLPF algorithms and to compare their accuracy with that of the other algorithm. The errors in pupil diameter data were smaller for the PRSSM and CCLPF algorithms than for the other algorithm.
Anxiety, Stress, and Social Support in Pregnant Women in the Province of Leon during COVID-19 Disease
Authors: García-Fernández Rubén , Liébana-Presa Cristina , Marqués-Sánchez Pilar , Martínez-Fernández María Cristina , Calvo-Ayuso Natalia , Hidalgo-Lopezosa Pedro
Published: April 2022

The COVID-19 pandemic has posed a high risk to the mental health of the entire population. Pregnant women are strongly affected by the consequences of COVID-19, resulting in increased anxiety and stress. Social support can be a protective factor when it comes to mental health disturbances such as anxiety, fear, or stress in pregnant women. This research aims to describe the anxiety and stress of women in the first trimester of pregnancy in times of pandemic and its relationship with social support. A quantitative, descriptive, cross-sectional study was conducted. A total of 115 women in the first trimester of pregnancy participated. Anxiety was found in 78.3% of the women. Self-perceived stress correlated significantly with the dimensions “concern for changes in oneself”, “feelings about oneself”, “concern about the future”, and very weakly with social support. In addition, a negative correlation was observed between “feelings about oneself” and social support. During the COVID-19 pandemic, anxiety levels of women in our population are elevated. Pregnant women during the first trimester of pregnancy showed higher levels of fear of childbirth and concern about the future than multiparous women. Increased social support and decreased stress seem to influence “feeling about oneself”.
Use of Piriformospora indica to Promote Growth of Strawberry Daughter Plants
Authors: Liu Wei , Tan Min , Qu Pengyan , Huo Chensi , Liang Wenjie , Li Runlin , Jia Yan , Fan Xinping , Cheng Chunzhen
Published: April 2022

As an endophytic fungus, the growth-promoting effects of Piriformospora indica have been widely confirmed in many of its host plants. In this study, we investigated the influences of P. indica colonization on the growth of the daughter plants of two strawberry cultivars, ‘Benihoppe’ and ‘Sweet Charlie.’ The results showed that the fungus colonization significantly promoted the growth of the daughter plants of both of the two strawberry varieties. Its colonization greatly improved almost all of the growth parameters of the ‘Benihoppe’ daughter plants, including the above-ground fresh weight, above-ground dry weight, root fresh weight, root dry weight, plant height, petiole length, leaf area, number of roots and chlorophyll content. However, the fungus colonization showed significant improving effects on only the above-ground fresh weight, root fresh weight and root dry weight of ‘Sweet Charlie.’ Surprisingly, the average root length of ‘Benihoppe’ and ‘Sweet Charlie’ was suppressed by about 14.3% and 24.6%, respectively, by P. indica. Moreover, after P. indica colonization, the leaf nitrate reductase activity and root activity upregulated by 30.12% and 12.74%, and 21.85% and 21.16%, respectively, for the ‘Benihoppe’ and ‘Sweet Charlie’ daughter plants. Our study indicated that P. indica could promote the growth of strawberry daughter plants by improving rooting, strengthening photosynthetic pigments production and nutrient absorption and accelerating biomass accumulation. The fungus shows great potential to be used in the strawberry industry, especially in the breeding of daughter plants.
Regional Ombrian Curves: Design Rainfall Estimation for a Spatially Diverse Rainfall Regime
Authors: Iliopoulou Theano , Malamos Nikolaos , Koutsoyiannis Demetris
Published: April 2022

Ombrian curves, i.e., curves linking rainfall intensity to return period and time scale, are well-established engineering tools crucial to the design against stormwaters and floods. Though the at-site construction of such curves is considered a standard hydrological task, it is a rather challenging one when large regions are of interest. Regional modeling of ombrian curves is particularly complex due to the need to account for spatial dependence together with the increased variability of rainfall extremes in space. We develop a framework for the parsimonious modeling of the extreme rainfall properties at any point in a given area. This is achieved by assuming a common ombrian model structure, except for a spatially varying scale parameter which is itself modeled by a spatial smoothing model for the 24 h average annual rainfall maxima that employs elevation as an additional explanatory variable. The fitting is performed on the pooled all-stations data using an advanced estimation procedure (K-moments) that allows both for reliable high-order moment estimation and simultaneous handling of space-dependence bias. The methodology is applied in the Thessaly region, a 13,700 km2 water district of Greece characterized by varying topography and hydrometeorological properties.
Dynamic Threshold Effect of Directed Technical Change Suppress on Urban Carbon Footprint in China
Authors: Lyu Xiaojun , Ke Haiqian
Published: April 2022

Promoting technical change is an important driving force for promoting the sustainable development of urban economy and ecology; however, the technical change is not always neutral and technical change may has a certain direction. This paper uses the DEA-Malmquist index to measure the directed technical change of 280 cities in China from 2009 to 2019, and uses the DMSP/OLS night light data to characterize the urban economic development level. It uses the dynamic threshold regression model to analyze the impact of directed technical change on urban carbon footprint under different economic development levels. The results show that: (1) during the study period, the carbon footprint of Chinese cities has a positive spatial correlation, and the direction of technical change is towards capital-saving overall. (2) The impact of capital-saving technical change on urban carbon footprint presents a negative double-threshold characteristic in China, and the inhibition of capital-saving technical change on the urban carbon footprint becomes stronger with the increasing economic development level. (3) The inhibitory effect of capital-saving technical change on carbon footprint has regional heterogeneity, and the inhibitory effect of capital-saving technical change on carbon footprint is stronger in eastern China than other regions. (4) Industrial structure, energy structure and innovation efficiency are mediating variables of the inhibitory effect of capital-saving technical change on carbon footprint except for population density.
Smartphones, the Epidemic of the 21st Century: A Possible Source of Addictions and Neuropsychiatric Consequences
Authors: Adamczewska-Chmiel Klaudia , Dudzic Katarzyna , Chmiela Tomasz , Gorzkowska Agnieszka
Published: April 2022

Background and Objectives: Phonoholism is the excessive and harmful use of a smartphone. We are now observing this phenomenon among adults more often. Using a smartphone for several hours may lead to somatic and psychological symptoms, such as headaches and depression. The aim of this study is to assess the prevalence of phonoholism and to assess the association between smartphone overuse and neuropsychiatric disorders. Materials and Methods: A total of 368 people (70.1% were woman), aged between 19 and 82 years (average age 26.1), took part in an anonymous questionnaire consisting of the following elements: Hospital Anxiety and Depression Scale (HADS), Mobile Phone Problem Use Scale (MPPUS-9), and original questions regarding headaches and sleep quality, along with a subjective assessment of the use of smartphones and an objective evaluation based on data from the applications “Stay Free” and “Screen Time”. Results: A total of 61 respondents (16.6%) obtained a score on the MPPUS-9 scale, which revealed their problematic use of mobile devices. Patients with phonoholism had significantly more headaches (85% vs. 58.7%, p = 0.027). Subjects with phonoholism had significantly shorter mean sleep duration (7.14 h vs. 7.42 h, p = 0.0475) and were less likely to feel sleepy during the day (43.33% vs. 59.73%, p = 0.0271). The group with phonoholism had significantly higher scores on the HADS-A anxiety scale (8.29 vs. 10.9, p = 0.015), but a statistical significance was not confirmed for depressive symptoms. Conclusions: The excessive use of the telephone negatively affects both somatic and mental health and can pose a significant clinical problem.
Volunteering among Chinese College Students during the COVID-19 Pandemic
Authors: Geng Yun , Cheung Shannon P. , Huang Chien-Chung , Liao Jinyu
Published: April 2022

Volunteering has been found to be not only beneficial to the well-being of recipients but also to the volunteers themselves, particularly from the life course perspective. Although previous studies have identified key factors of volunteering motivation, the literature is less focused on the interplay of public interest and private gains in volunteering motivation. This study used 1871 college students across China to examine how the interplay between public interest and private gains affects general and Coronavirus Disease 2019 (COVID-19)-specific volunteering during the pandemic. The results show that the interplay of these two factors constitutes a dynamic process, depending on the volunteering and time-specific context. Overall, undergraduate students with greater concern for public interest and less preference in private gains had the highest rate of overall volunteering, followed by students with high concern for both public interest and private gains. It is crucial to take both public interest and private gains into account when discussing volunteering opportunities among Chinese college students, which may increase the well-being of students in the long run.
Theranostic Agent Combining Fullerene Nanocrystals and Gold Nanoparticles for Photoacoustic Imaging and Photothermal Therapy
Authors: Kawasaki Riku , Kondo Kosuke , Miura Risako , Yamana Keita , Isozaki Hinata , Shimada Risako , Kawamura Shogo , Hirano Hidetoshi , Nishimura Tomoki , Tarutani Naoki , Katagiri Kiyofumi , Stubelius Alexandra , Sawada Shin-ichi , Sasaki Yoshihiro , Akiyoshi Kazunari , Ikeda Atsushi
Published: April 2022

Developing photoactivatable theranostic platforms with integrated functionalities of biocompatibility, targeting, imaging contrast, and therapy is a promising approach for cancer diagnosis and therapy. Here, we report a theranostic agent based on a hybrid nanoparticle comprising fullerene nanocrystals and gold nanoparticles (FGNPs) for photoacoustic imaging and photothermal therapy. Compared to gold nanoparticles and fullerene crystals, FGNPs exhibited stronger photoacoustic signals and photothermal heating characteristics by irradiating light with an optimal wavelength. Our studies demonstrated that FGNPs could kill cancer cells due to their photothermal heating characteristics in vitro. Moreover, FGNPs that are accumulated in tumor tissue via the enhanced permeation and retention effect can visualize tumor tissue due to their photoacoustic signal in tumor xenograft model mice. The theranostic agent with FGNPs shows promise for cancer therapy.
Protein Phosphatase 2A Improves Cardiac Functional Response to Ischemia and Sepsis
Authors: Gergs Ulrich , Jahn Tina , Schulz Nico , Großmann Claudia , Rueckschloss Uwe , Demus Uta , Buchwalow Igor B. , Neumann Joachim
Published: April 2022

Reversible protein phosphorylation is a posttranslational modification of regulatory proteins involved in cardiac signaling pathways. Here, we focus on the role of protein phosphatase 2A (PP2A) for cardiac gene expression and stress response using a transgenic mouse model with cardiac myocyte-specific overexpression of the catalytic subunit of PP2A (PP2A-TG). Gene and protein expression were assessed under basal conditions by gene chip analysis and Western blotting. Some cardiac genes related to the cell metabolism and to protein phosphorylation such as kinases and phosphatases were altered in PP2A-TG compared to wild type mice (WT). As cardiac stressors, a lipopolysaccharide (LPS)-induced sepsis in vivo and a global cardiac ischemia in vitro (stop-flow isolated perfused heart model) were examined. Whereas the basal cardiac function was reduced in PP2A-TG as studied by echocardiography or as studied in the isolated work-performing heart, the acute LPS- or ischemia-induced cardiac dysfunction deteriorated less in PP2A-TG compared to WT. From the data, we conclude that increased PP2A activity may influence the acute stress tolerance of cardiac myocytes.
Drought-Stress Induced Physiological and Molecular Changes in Plants
Authors: Hura Tomasz , Hura Katarzyna , Ostrowska Agnieszka
Published: April 2022

Soil drought is one of the major abiotic stresses that inhibits the growth, development, and yield of crops all over the world [...]
A Contrastive Learning Pre-Training Method for Motif Occupancy Identification
Authors: Lin Ken , Quan Xiongwen , Yin Wenya , Zhang Han
Published: April 2022

Motif occupancy identification is a binary classification task predicting the binding of DNA motif instances to transcription factors, for which several sequence-based methods have been proposed. However, through direct training, these end-to-end methods are lack of biological interpretability within their sequence representations. In this work, we propose a contrastive learning method to pre-train interpretable and robust DNA encoding for motif occupancy identification. We construct two alternative models to pre-train DNA sequential encoder, respectively: a self-supervised model and a supervised model. We augment the original sequences for contrastive learning with edit operations defined in edit distance. Specifically, we propose a sequence similarity criterion based on the Needleman–Wunsch algorithm to discriminate positive and negative sample pairs in self-supervised learning. Finally, a DNN classifier is fine-tuned along with the pre-trained encoder to predict the results of motif occupancy identification. Both proposed contrastive learning models outperform the baseline end-to-end CNN model and SimCLR method, reaching AUC of 0.811 and 0.823, respectively. Compared with the baseline method, our models show better robustness for small samples. Specifically, the self-supervised model is proved to be practicable in transfer learning.
Studies on Red Mud Material to Use for Combustion of Vietnam Pulverized Coal
Authors: Nguyen Thi Thuc Phuong , Nguyen Van Tung , Hoang Nhuan , Hoang Van Duc , Luu Xuan Dinh , Le Thi Mai Huong , Nguyen Trong Hung , Hoang Ngoc Bich , Tran Thi Kim Ngan , Chu Manh Nhuong
Published: April 2022

The catalytic effect of red mud on Vietnam anthracite’s combustion characteristics was investigated. The mineralogical composition of the red mud includes CaCO3, Fe2O3, FeO(OH), FeTiO3, and Al(OH)3. This red mud is rich in Na, Ca, Al, Fe, and Ti. The combustion characteristics were analyzed by the thermogravimetry method. The combustion effectiveness was assessed by thermogravimetric analysis. The results were derived from a combination of several parameters, such as the ignition temperature, the burnout efficiency, and the amount of heat release. The combustion characteristics of pulverized coal were improved by the introduction of red mud, and the greatest catalytic performance was achieved when the content reached 6%. With the optimal addition, the ignition temperature of anthracite was reduced by 12 °C, and the burnout efficiencies were increased by 2.59% compared to raw anthracite. The amount of heat released by anthracite was increased to 6.93 kJ/g by adding red mud.
Genome-Wide Identification and Analysis of Lipases in Fig Wasps (Chalcidoidea, Hymenoptera)
Authors: Wei Xianqin , Li Jiaxing , Wang Tao , Xiao Jinhua , Huang Dawei
Published: April 2022

Lipases are the main enzymes involved in lipid metabolism. However, the characteristics of lipases in insects were scarcely investigated. Here, we screened the recently sequenced genomes of 12 fig wasp species consisting of seven pollinator fig wasps (PFWs) and five non-pollinating fig wasps (NPFWs) for the six major lipase gene families. In total, 481 lipase genes were identified, and the two most numerous families were the neutral and acid lipases. Tandem duplication accounted for the expansion of the gene family. NPFWs had significantly more lipases than PFWs. A significant gene family contraction occurred in the clade of PFWs. The difference of lipases between NPFWs and PFWs might contribute to their distinction in life histories and feeding regimes. Phylogenetic analysis showed that the lipase genes of each fig wasp species was almost equally distributed in each clade, indicating that the lipase genes were conserved. The gene structures were similar within each clade, while they were different among clades. Most of the neutral and acid lipases were signal peptides and located extracellularly. The pathways of lipases involved were predicted. This genome-wide study provides a systematic analysis of lipase gene families in 12 hymenopteran insects and further insights towards understanding the potential functions of lipases.
The Perivascular Fat Attenuation Index Improves the Diagnostic Performance for Functional Coronary Stenosis
Authors: Yan Hankun , Zhao Na , Geng Wenlei , Hou Zhihui , Gao Yang , Lu Bin
Published: April 2022

Background: Coronary computed tomography angiography (CCTA) is an established first-line test in the investigation of patients with suspected coronary artery disease (CAD), while the perivascular fat attenuation index (FAI) derived from CT seems to be a feasible and efficient tool for the identification of ischemia. The association between the FAI and lesion-specific ischemia as assessed by fractional flow reserve (FFR) remains unclear. Methods: In a total of 261 patients, 294 vessels were assessed for CCTA stenosis, vessel-specific FAI, lesion-specific FAI, and plaque characteristics. The diagnostic accuracies of each parameter and the combined approach were analyzed via the receiver operating characteristic curve (ROC) with FFR as the reference standard. The determinants of FAI were statistically analyzed. Results: The cutoff values of vessel-specific FAI and lesion-specific FAI scores calculated according to the Youden index were −70.97 and −73.95 HU, respectively. No significant differences were noted between them; however, they exhibited a strong correlation. No significant differences were noted between the area under the curve (AUC) scores of vessel-specific FAI (0.677), lesion-specific FAI (0.665), and CCTA (0.607) (p > 0.05 for all) results. The addition of two FAI measures to the CCTA showed improvements in the discrimination (AUC) and reclassification ability (relative integrated discrimination improvement (IDI) and category-free net reclassification index (NRI)), vessel-specific FAI (AUC, 0.696; NRI, 49.6%; IDI, 5.9%), and lesion-specific FAI scores (AUC, 0.676; NRI, 43.3%; IDI, 5.4%); (p < 0.01 for all). Multivariate analysis revealed that low-attenuation plaque (LAP) volume was an independent predictor of two FAI measures. Conclusion: The combined approach of adding vessel-specific FAI or lesion-specific FAI scores could improve the identification of ischemia compared with CCTA alone. The LAP volume was the independent risk factor for both tools.
Quality of Life and Excessive Daytime Sleepiness in Adults with Obstructive Sleep Apnea Who Are Treated with Multilevel Surgery or Adherent to Continuous Positive Airway Pressure
Authors: Iannella Giannicola , Magliulo Giuseppe , Lo Iacono Cristina Anna Maria , Visconti Irene Claudia , Lechien Jerome R. , Perrone Tiziano , Cammaroto Giovanni , Meccariello Giuseppe , Maniaci Antonino , Cocuzza Salvatore , Di Luca Milena , De Vito Andrea , Martone Chiara , Polimeni Antonella , Greco Antonio , de Vincentiis Marco , Ralli Massimo , Pace Annalisa , Gulotta Giampiero , Pelucchi Stefano , Eplite Angelo , Vicini Claudio
Published: April 2022

Obstructive Sleep Apnea (OSA) syndrome is a respiratory sleep disorder characterized by a reduction (hypopnea) in or a complete cessation (apnea) of airflow in the upper airways at night, in the presence of breathing effort. The gold standard treatment for OSA is ventilation through continuous positive airway pressure (CPAP), although this often shows poor patient compliance. In recent years, transoral robotic surgery (TORS) has been proposed as a valid surgical treatment for patients suffering from OSA in a multilevel surgical setting. The aim of this study is to analyze the effects on QoL and daytime sleepiness of multilevel surgery for OSA (barbed pharyngoplasty + transoral robotic surgery). Furthermore, we compared the impact on QoL and daytime sleepiness of two different treatments for patients with moderate to severe OSA, such as CPAP and TORS. Sixty-seven OSA patients who underwent multilevel robotic surgery and sixty-seven OSA patients treated with CPAP were enrolled, defined as Group 1 and Group 2, respectively. The Glasgow Benefit Inventory (GBI) questionnaire was administrated to evaluate the changes in the QoL. Respiratory outcomes were evaluated and compared. Group 1 showed a GBI total average value of +30.4, whereas Group 2, a value of +33.2 (p = 0.4). General benefit score showed no difference between groups (p = 0.1). Better values of social status benefit (p = 0.0006) emerged in the CPAP Group, whereas greater physical status benefit (p = 0.04) was shown in the TORS Group. Delta-AHI (−23.7 ± 14.3 vs. −31.7 ± 15.6; p = 0.001) and Delta-ODI (−24.5 ± 9.5 vs. −29.4 ± 10.5; p = 0.001) showed better values in the CPAP group. Therapeutic success rate of the Multilevel TORS Group was 73.1% and 91% in the CPAP group (p = 0.01), respectively. Multilevel TORS and CPAP have a positive effect on the quality of life of OSA patients. Greater social support has been reported in the CPAP group and better physical health status in the TORS group. No statistical difference emerged in the reduction in daytime sleepiness between both groups.
Predictors of Submaximal Exercise Test Attainment in Adults Reporting Long COVID Symptoms
Authors: Romero-Ortuno Roman , Jennings Glenn , Xue Feng , Duggan Eoin , Gormley John , Monaghan Ann
Published: April 2022

Adults with long COVID often report intolerance to exercise. Cardiopulmonary exercise testing (CPET) has been used in many settings to measure exercise ability but has been conducted in a few long COVID cohorts. We conducted CPET in a sample of adults reporting long COVID symptoms using a submaximal cycle ergometer protocol. We studied pre-exercise predictors of achieving 85% of the age-predicted maximum heart rate (85%HRmax) using logistic regression. Eighty participants were included (mean age 46 years, range 25–78, 71% women). Forty participants (50%) did not reach 85%HRmax. On average, non-achievers reached 84% of their predicted 85%HRmax. No adverse events occurred. Participants who did not achieve 85%HRmax were older (p < 0.001), had more recent COVID-19 illness (p = 0.012) with higher frequency of hospitalization (p = 0.025), and had been more affected by dizziness (p = 0.041) and joint pain (p = 0.028). In the logistic regression model including age, body mass index, time since COVID-19, COVID-19-related hospitalization, dizziness, joint pain, pre-existing cardiopulmonary disease, and use of beta blockers, independent predictors of achieving 85%HRmax were younger age (p = 0.001) and longer time since COVID-19 (p = 0.008). Our cross-sectional findings suggest that exercise tolerance in adults with long COVID has potential to improve over time. Longitudinal research should assess the extent to which this may occur and its mechanisms. ClinicalTrials.gov identifier: NCT05027724 (TROPIC Study).
Effects of Type 2 Diabetes Mellitus on Osteoclast Differentiation, Activity, and Cortical Bone Formation in POSTmenopausal MRONJ Patients
Authors: Park Sung-Min , Lee Jae-Hoon
Published: April 2022

Osteoporosis is a common metabolic bone disease in patients with diabetes, which can develop simultaneously with type 2 diabetes (T2D) in postmenopausal women. Bisphosphonate (BP) is administered to patients with both conditions and may cause medication-related osteonecrosis of the jaw (MRONJ). It affects the differentiation and function of osteoclasts as well as the thickness of the cortical bone through bone mineralization. Therefore, this study aimed to investigate the effects of T2D on osteoclast differentiation and activity as well as cortical bone formation in postmenopausal patients with MRONJ. Tissue samples were collected from 10 patients diagnosed with T2D and stage III MRONJ in the experimental group and from 10 patients without T2D in the control group. A histological examination was conducted, and the expression of dendritic cell-specific transmembrane protein (DC-STAMP) and tartrate-resistant acid phosphatase (TRAP) was assessed. Cortical bone formation was analyzed using CBCT images. The number of TRAP-positive osteoclasts and DC-STAMP-positive mononuclear cells was significantly less in the experimental group (p < 0.05). Furthermore, the thickness and ratio of cortical bone were significantly greater in the experimental group (p < 0.05). In conclusion, T2D decreased the differentiation and function of osteoclasts and increased cortical bone formation in postmenopausal patients with MRONJ.
Dermatochalasis Aggravates Meibomian Gland Dysfunction Related Dry Eyes
Authors: Wu Wan-Lin , Chang Shu-Wen
Published: April 2022

This study aimed to investigate the relationships between subjective symptoms, objective signs, and dermatochalasis severity in dry-eye patients and the effects of lid hygiene on dry-eye parameters. We retrospectively enrolled 2328 patients who underwent dry-eye examinations and classified them into four groups by dermatochalasis severity. The SPEED and OSDI questionnaires were used to evaluate subjective symptoms. LipiView® II interferometry was used to measure lipid-layer thickness (LLT) and blink/incomplete blink rates and perform meibography. A slit-lamp–aided standardized evaluator measured meibomian gland expressibility (MGE). A meiboscale was used to grade meibomian gland dropout. Fluorescein tear-film break-up time (FTBUT) and superficial punctate keratitis (SPK) were recorded. The Schirmer test II with anesthetics was used to evaluate aqueous tear secretion. The effects of lid hygiene were evaluated in 644 patients who underwent second comprehensive examinations. The median age of patients was 55.3 [46.0–66.0] years (76.0% female). Patients with more severe dermatochalasis were less symptomatic and had less MGE, higher meiboscale grades and average LLT. Dermatochalasis severity was significantly associated with MGE and meiboscale grade in the upper lid. There were no significant differences in the Schirmer test, FTBUT, and SPK among the severity groups. Females were older and had higher LLT and less severe dermatochalasis. Lid hygiene significantly decreased subjective symptoms, LLT, and Schirmer results, increased FTBUT, but did not change MGE or meiboscale grades. Dermatochalasis severity participated in the pathophysiology of dry eyes. Lid hygiene significantly improved subjective symptoms and reduced LLT, more significantly in patients with less severe dermatochalasis.
Optimizing the Outcomes of Percutaneous Coronary Intervention in Patients with Chronic Kidney Disease
Authors: Caracciolo Alessandro , Scalise Renato Francesco Maria , Ceresa Fabrizio , Bagnato Gianluca , Versace Antonio Giovanni , Licordari Roberto , Perfetti Silvia , Lofrumento Francesca , Irrera Natasha , Santoro Domenico , Patanè Francesco , Di Bella Gianluca , Costa Francesco , Micari Antonio
Published: April 2022

Percutaneous coronary intervention (PCI) is one of the most common procedures performed in medicine. However, its net benefit among patients with chronic kidney disease (CKD) is less well established than in the general population. The prevalence of patients suffering from both CAD and CKD is high, and is likely to increase in the coming years. Planning the adequate management of this group of patients is crucial to improve their outcome after PCI. This starts with proper preparation before the procedure, the use of all available means to reduce contrast during the procedure, and the implementation of modern strategies such as radial access and drug-eluting stents. At the end of the procedure, personalized antithrombotic therapy for the patient’s specific characteristics is advisable to account for the elevated ischemic and bleeding risk of these patients.