bluuebunny/crossref_metadata_2025 huggingface dataset:
Automated Classification of Mental Arithmetic Tasks Using Recurrent Neural Network and Entropy Features Obtained from Multi-Channel EEG Signals
Authors: Varshney Abhishek , Ghosh Samit Kumar , Padhy Sibasankar , Tripathy Rajesh Kumar , Acharya U. Rajendra
Published: May 2021

The automated classification of cognitive workload tasks based on the analysis of multi-channel EEG signals is vital for human–computer interface (HCI) applications. In this paper, we propose a computerized approach for categorizing mental-arithmetic-based cognitive workload tasks using multi-channel electroencephalogram (EEG) signals. The approach evaluates various entropy features, such as the approximation entropy, sample entropy, permutation entropy, dispersion entropy, and slope entropy, from each channel of the EEG signal. These features were fed to various recurrent neural network (RNN) models, such as long-short term memory (LSTM), bidirectional LSTM (BLSTM), and gated recurrent unit (GRU), for the automated classification of mental-arithmetic-based cognitive workload tasks. Two cognitive workload classification strategies (bad mental arithmetic calculation (BMAC) vs. good mental arithmetic calculation (GMAC); and before mental arithmetic calculation (BFMAC) vs. during mental arithmetic calculation (DMAC)) are considered in this work. The approach was evaluated using the publicly available mental arithmetic task-based EEG database. The results reveal that our proposed approach obtained classification accuracy values of 99.81%, 99.43%, and 99.81%, using the LSTM, BLSTM, and GRU-based RNN classifiers, respectively for the BMAC vs. GMAC cognitive workload classification strategy using all entropy features and a 10-fold cross-validation (CV) technique. The slope entropy features combined with each RNN-based model obtained higher classification accuracy compared with other entropy features for the classification of the BMAC vs. GMAC task. We obtained the average classification accuracy values of 99.39%, 99.44%, and 99.63% for the classification of the BFMAC vs. DMAC tasks, using the LSTM, BLSTM, and GRU classifiers with all entropy features and a hold-out CV scheme. Our developed automated mental arithmetic task system is ready to be tested with more databases for real-world applications.
Hard Rock Mining Using Disk Tools Supported by High-Pressure Water Jets in the Aspect of Reducing Energy Consumption
Authors: Kotwica Krzysztof
Published: May 2021

The mining of hard rocks by mechanical methods, using cutting tools, is associated with problems such as sparking, dust generation and increased wear. The application of disk tools allows limiting these problems but requires constructing a machine with large dimensions and weight. To limit these problems, high-pressure water jets can be applied. The article presents the effects of using this solution in the field and stand tests when mining rocks with a symmetrical disk. The results of the bench tests of high-pressure water jets to support the process of mining with symmetrical disks, carried out at the AGH University of Science and Technology, performed on a unique test stand, which enabled the samples to be mined in a circular trajectory in conditions similar to real ones, are presented. With the use of a dimensional analysis, a theoretical-empirical model was developed to estimate the impact of slots cut by water jets on the tool load and the amount of output as a function of slots spacing and depth. Considering the similarity criteria for the mined rock sample (mechanical properties), it is possible, with the use of this model, to estimate the most advantageous working parameters while hydromechanical mining uses disks on a larger cutting radius.
A Flow Rate Dependent 1D Model for Thermally Stratified Hot-Water Energy Storage
Authors: Rendall Joseph , Karg Bulnes Fernando , Gluesenkamp Kyle , Abu-Heiba Ahmad , Worek William , Nawaz Kashif
Published: May 2021

Stratified tank models are used to simulate thermal storage in applications such as residential or commercial hot-water storage tanks, chilled-water storage tanks, and solar thermal systems. The energy efficiency of these applications relates to the system components and the level of stratification maintained during various flow events in the tank. One-dimensional (1D) models are used in building energy simulations because of the short computation time but often do not include flow-rate dependent mixing. The accuracy of 1D models for plug flow, plug flow with axial conduction, and two convection eddy-diffusivity models were compared with experimental data sets for discharging a 50-gal residential tank and recharging the tank with hot water from an external hot-water source. A minimum and maximum relationship for the eddy diffusivity factor were found at Re <2100 and >10,000 for recirculation of hot water to the top of the tank and vertical tubes inletting cold water at the bottom. The root mean square error decreased from >4 °C to near 2 °C when considering flow-based mixing models during heating, while the exponential decay of the eddy diffusion results in a root mean square error reduction of 1 °C for cone-shaped diffusers that begin to relaminarize flow at the inlet.
An Experimental-Numerical Investigation of the Wake Structure of a Hovering Rotor by PIV Combined with a Γ2 Vortex Detection Criterion
Authors: De Gregorio Fabrizio , Visingardi Antonio , Iuso Gaetano
Published: May 2021

The rotor wake aerodynamic characterization is a fundamental aspect for the development and optimization of future rotary-wing aircraft. The paper is aimed at experimentally and numerically characterizing the blade tip vortices of a small-scale four-bladed isolated rotor in hover conditions. The investigation of the vortex decay process during the downstream convection of the wake is addressed. Two-component PIV measurements were carried out below the rotor disk down to a distance of one rotor radius. The numerical simulations were aimed at assessing the modelling capabilities and the accuracy of a free-wake Boundary Element Methodology (BEM). The experimental and numerical results were investigated by the Γ2 criterion to detect the vortex location. The rotor wake mean velocity field and the instantaneous vortex characteristics were investigated. The experimental/numerical comparisons show a reasonable agreement in the estimation of the mean velocity inside the rotor wake, whereas the BEM predictions underestimate the diffusion effects. The numerical simulations provide a clear picture of the filament vortex trajectory interested in complex interactions starting at about a distance of z/R = −0.5. The time evolution of the tip vortices was investigated in terms of net circulation and swirl velocity. The PIV tip vortex characteristics show a linear mild decay up to the region interested by vortex pairing and coalescence, where a sudden decrease, characterised by a large data scattering, occurs. The numerical modelling predicts a hyperbolic decay of the swirl velocity down to z/R = −0.4 followed by an almost constant decay. Instead, the calculated net circulation shows a gradual decrease throughout the whole wake development. The comparisons show discrepancies in the region immediately downstream the rotor disk but significant similarities beyond z/R = −0.5.
Twisted Silica Microstructured Optical Fiber with Equiangular Spiral Six-Ray Geometry
Authors: Bourdine Anton V. , Barashkin Alexey Yu. , Burdin Vladimir A. , Dashkov Michael V. , Demidov Vladimir V. , Dukelskii Konstantin V. , Evtushenko Alexander S. , Ismail Yaseera , Khokhlov Alexander V. , Kuznetsov Artem A. , Matrosova Alexandra S. , Morozov Oleg G. , Pchelkin Grigori A. , Petruccione Francesco , Sakhabutdinov Airat Zh. , Singh Ghanshyam , Ter-Nersesyants Egishe V. , Tiwari Manish , Zaitseva Elena S. , Janyani Vijay , Yin Juan
Published: May 2021

This work presents fabricated silica microstructured optical fiber with special equiangular spiral six-ray geometry, an outer diameter of 125 µm (that corresponds to conventional commercially available telecommunication optical fibers of ratified ITU-T recommendations), and induced chirality with twisting of 200 revolutions per minute (or e.g., under a drawing speed of 3 m per minute, 66 revolutions per 1 m). We discuss the fabrication of twisted microstructured optical fibers. Some results of tests, performed with pilot samples of designed and manufactured stellar chiral silica microstructured optical fiber, including basic transmission parameters, as well as measurements of near-field laser beam profile and spectral and pulse responses, are represented.
Impact of Lacticaseibacillus rhamnosus GG on the Emulsion Stability of Raw Milk
Authors: Dos Santos Morais Raphael , Louvet Nicolas , Borges Frederic , Dumas Dominique , Cvetkovska-Ben Mohamed Loubiana , Barrau Sarah , Scher Joël , Gaiani Claire , Burgain Jennifer
Published: May 2021

Lactic acid bacteria (LAB) have been studied for several decades to understand and determine their mechanism and interaction within the matrix into which they are introduced. This study aimed to determine the spatial distribution of Lacticaseibacillus rhamnosus GG (LGG) in a dairy matrix and to decipher its behaviour towards milk components, especially fat globules. Two strains of this widely studied bacterium with expected probiotic effects were used: LGG WT with pili on the cell surface and its pili-depleted mutant—LGG ΔspaCBA—in order to determine the involvement of these filamentous proteins. In this work, it was shown that LGG ΔspaCBA was able to limit creaming with a greater impact than the wild-type counterpart. Moreover, confocal imaging evidenced a preferential microbial distribution as aggregates for LGG WT, while the pili-depleted strain tended to be homogenously distributed and found as individual chains. The observed differences in creaming are attributed to the indirect implication of SpaCBA pili. Indeed, the bacteria-to-bacteria interaction surpassed the bacteria-to-matrix interaction, reducing the bacterial surface exposed to raw milk. Conversely, LGG ΔspaCBA may form a physical barrier responsible for preventing milk fat globules from rising to the surface.
Indicators and Recommendations for Assessing Sustainable Healthy Diets
Authors: Aldaya Maite M. , Ibañez Francisco C. , Domínguez-Lacueva Paula , Murillo-Arbizu María Teresa , Rubio-Varas Mar , Soret Beatriz , Beriain María José
Published: May 2021

Research coupling human nutrition and sustainability concerns is a rapidly developing field, which is essential to guide governments’ policies. This critical and comprehensive review analyzes indicators and approaches to “sustainable healthy diets” published in the literature since this discipline’s emergence a few years ago, identifying robust gauges and highlighting the flaws of the most commonly used models. The reviewed studies largely focus on one or two domains such as greenhouse gas emissions or water use, while overlooking potential impact shifts to other sectors or resources. The present study covers a comprehensive set of indicators from the health, environmental and socio-economic viewpoints. This assessment concludes that in order to identify the best food option in sustainability assessments and nutrition analysis of diets, some aspects such as the classification and disaggregation of food groups, the impacts of the rates of local food consumption and seasonality, preservation methods, agrobiodiversity and organic food and different production systems, together with consequences for low-income countries, require further analysis and consideration.
Volatile Composition of Industrially Fermented Table Olives from Greece
Authors: Mikrou Theano , Kasimati Katerina , Doufexi Ioanna , Kapsokefalou Maria , Gardeli Chrysavgi , Mallouchos Athanasios
Published: May 2021

Table olives represent one of the most important fermented products in Greece. Their highly appreciated flavor is directly associated with the volatile composition. However, extensive data on the volatile profile of table olives from Greek cultivars are scarce in the literature. For this reason, the volatile components of industrially fermented table olives from Kalamata, Conservolea and Halkidiki cultivars grown in different geographical areas within Greece were determined using headspace solid-phase microextraction combined with gas chromatography–mass spectrometry. More than 100 volatile compounds were identified and distributed over different chemical classes. All samples were rich in esters, alcohols and acids, whereas the samples of cv. Halkidiki were also characterized by increased levels of volatile phenols. Both qualitative and quantitative differences were observed, which resulted in the discrimination of the table olives according to olive cultivar and growing location. To the best of our knowledge, this is the first systematic study on the volatile profiles of table olives from Greek cultivars that also highlights the pronounced effect of olives’ growing location.
Electroactive Polymeric Composites to Mimic the Electromechanical Properties of Myocardium in Cardiac Tissue Repair
Authors: Meyers Kaylee , Lee Bruce P. , Rajachar Rupak M.
Published: May 2021

Due to the limited regenerative capabilities of cardiomyocytes, incidents of myocardial infarction can cause permanent damage to native myocardium through the formation of acellular, non-conductive scar tissue during wound repair. The generation of scar tissue in the myocardium compromises the biomechanical and electrical properties of the heart which can lead to further cardiac problems including heart failure. Currently, patients suffering from cardiac failure due to scarring undergo transplantation but limited donor availability and complications (i.e., rejection or infectious pathogens) exclude many individuals from successful transplant. Polymeric tissue engineering scaffolds provide an alternative approach to restore normal myocardium structure and function after damage by acting as a provisional matrix to support cell attachment, infiltration and stem cell delivery. However, issues associated with mechanical property mismatch and the limited electrical conductivity of these constructs when compared to native myocardium reduces their clinical applicability. Therefore, composite polymeric scaffolds with conductive reinforcement components (i.e., metal, carbon, or conductive polymers) provide tunable mechanical and electroactive properties to mimic the structure and function of natural myocardium in force transmission and electrical stimulation. This review summarizes recent advancements in the design, synthesis, and implementation of electroactive polymeric composites to better match the biomechanical and electrical properties of myocardial tissue.
Hippocampal Subregion and Gene Detection in Alzheimer’s Disease Based on Genetic Clustering Random Forest
Authors: Li Jin , Liu Wenjie , Cao Luolong , Luo Haoran , Xu Siwen , Bao Peihua , Meng Xianglian , Liang Hong , Fang Shiaofen
Published: May 2021

The distinguishable subregions that compose the hippocampus are differently involved in functions associated with Alzheimer’s disease (AD). Thus, the identification of hippocampal subregions and genes that classify AD and healthy control (HC) groups with high accuracy is meaningful. In this study, by jointly analyzing the multimodal data, we propose a novel method to construct fusion features and a classification method based on the random forest for identifying the important features. Specifically, we construct the fusion features using the gene sequence and subregions correlation to reduce the diversity in same group. Moreover, samples and features are selected randomly to construct a random forest, and genetic algorithm and clustering evolutionary are used to amplify the difference in initial decision trees and evolve the trees. The features in resulting decision trees that reach the peak classification are the important “subregion gene pairs”. The findings verify that our method outperforms well in classification performance and generalization. Particularly, we identified some significant subregions and genes, such as hippocampus amygdala transition area (HATA), fimbria, parasubiculum and genes included RYR3 and PRKCE. These discoveries provide some new candidate genes for AD and demonstrate the contribution of hippocampal subregions and genes to AD.
Genome-Wide Analysis of Sex Disparities in the Genetic Architecture of Lung and Colorectal Cancers
Authors: Nazarian Alireza , Kulminski Alexander M.
Published: May 2021

Almost all complex disorders have manifested epidemiological and clinical sex disparities which might partially arise from sex-specific genetic mechanisms. Addressing such differences can be important from a precision medicine perspective which aims to make medical interventions more personalized and effective. We investigated sex-specific genetic associations with colorectal (CRCa) and lung (LCa) cancers using genome-wide single-nucleotide polymorphisms (SNPs) data from three independent datasets. The genome-wide association analyses revealed that 33 SNPs were associated with CRCa/LCa at P < 5.0 × 10−6 neither males or females. Of these, 26 SNPs had sex-specific effects as their effect sizes were statistically different between the two sexes at a Bonferroni-adjusted significance level of 0.0015. None had proxy SNPs within their ±1 Mb regions and the closest genes to 32 SNPs were not previously associated with the corresponding cancers. The pathway enrichment analyses demonstrated the associations of 35 pathways with CRCa or LCa which were mostly implicated in immune system responses, cell cycle, and chromosome stability. The significant pathways were mostly enriched in either males or females. Our findings provided novel insights into the potential sex-specific genetic heterogeneity of CRCa and LCa at SNP and pathway levels.
Genetic Alterations and Therapeutic Targeting of Philadelphia-Like Acute Lymphoblastic Leukemia
Authors: Iacobucci Ilaria , Roberts Kathryn G.
Published: May 2021

Philadelphia-like (Ph-like) acute lymphoblastic leukemia (ALL) is a subgroup of B-cell precursor ALL which by gene expression analysis clusters with Philadelphia-positive ALL although lacking the pathognomonic BCR-ABL1 oncoprotein. Its prevalence increases with age and similar to BCR-ABL1-positive ALL, Ph-like ALL is characterized by IKZF1 or other B-lymphoid transcription factor gene deletions and by poor outcome to conventional therapeutic approaches. Genetic alterations are highly heterogenous across patients and include gene fusions, sequence mutations, DNA copy number changes and cryptic rearrangements. These lesions drive constitutively active cytokine receptor and kinase signaling pathways which deregulate ABL1 or JAK signaling and more rarely other kinase-driven pathways. The presence of activated kinase alterations and cytokine receptors has led to the incorporation of targeted therapy to the chemotherapy backbone which has improved treatment outcome for this high-risk subtype. More recently, retrospective studies have shown the efficacy of immunotherapies including both antibody drug-conjugates and chimeric antigen receptor T cell therapy and as they are not dependent on a specific genetic alteration, it is likely their use will increase in prospective clinical trials. This review summarizes the genomic landscape, clinical features, diagnostic assays, and novel therapeutic approaches for patients with Ph-like ALL.
Calcerous Tufa as Invaluable Geotopes Endangered by (Over-)Tourism: A Case Study in the UNESCO Global Geopark Swabian Alb, Germany
Authors: Megerle Heidi Elisabeth
Published: May 2021

Calcerous tufa and sinter are among the most impressive natural spectacles in karst landscapes whose scientific and aesthetic value is universally recognized. Being visually often very appealing they attract numerous visitors. At the same time tufa landforms are extremely vulnerable and can be seriously damaged even by minor interference. The challenge is, therefore, to protect the calcerous tufa heritage, to communicate its values, and to enhance it with the help of adequate geotourism offers. Tufa geotopes are an essential part of the geological heritage of the UNESCO Global Geopark Swabian Alb in Southwest Germany. Unfortunately tufa landforms, especially tufa cascades, suffer serious impairments by (over-)tourism, particularly during the Corona pandemic. The article explores where best to strike the balance between valorization and protection, as well as how to ensure that growth in tourism is compatible with nature preservation, especially in the case of the extremely vulnerable tufa geotopes.
The Effect of Trunk Stability Training Based on Visual Feedback on Trunk Stability, Balance, and Upper Limb Function in Stroke Patients: A Randomized Control Trial
Authors: Yang Seok-Hui , Chung Eun-Jung , Lee Jin , Lee Su-Hyun , Lee Byoung-Hee
Published: May 2021

This study aimed to investigate the effects of trunk stability training based on visual feedback on trunk stability, balance, and upper limb function in patients with stroke. Twenty-eight patients with chronic stroke were randomly assigned to either a trunk support group (n = 14) or a trunk restraint group (n = 14) that practiced upper limb training with trunk support and trunk restraint, respectively, based on visual feedback for 30 min per day, three times per week, for 4 weeks. The postural assessment scale for stroke (PASS) was used to assess the stability of patients, and the functional reaching test (FRT) was performed to assess balance. To assess upper extremity function, a range of motion (ROM) test, manual muscle testing (MMT), and Fugl–Meyer assessment-upper limb (FMA-upper limb) were performed. Consequently, both groups showed significant differences before and after training in the PASS, FRT, shoulder flexion ROM, triceps brachii MMT, and FMA-upper limb (p < 0.05), while the trunk support group showed more significant improvements than the trunk restraint group in the PASS, FRT, and FMA-upper limb (p < 0.05). Trunk support-based upper limb training effectively improved trunk stability, balance, and upper limb function and is beneficial as an upper limb training method. Providing trunk support is more effective than restricting the trunk; trunk support-based upper limb training is expected to promote voluntary participation when combined with visual feedback.
Genome-Wide Identification and Expression Analysis of MYB Transcription Factors and Their Responses to Abiotic Stresses in Woodland Strawberry (Fragaria vesca)
Authors: Li Huihui , Zhou Yanwei , Ma Zongxin , Lu Xiaoqing , Li Yunlong , Chen Hong
Published: May 2021

Woodland strawberry (Fragaria vesca) is a diploid strawberry that is widely used as a model of cultivated octoploid strawberry (Fragaria × ananassa). It has also been used as a model for Rosaceae fruits, non-climacteric fruits, and stolons. The MYB superfamily is the largest transcription factor family in plants, and its members play important roles in plant growth and development. However, the complete MYB superfamily in woodland strawberry has not been studied. In this study, a total of 217 MYB genes were identified in woodland strawberry and classified into four groups: one 4R-MYB protein, five 3R-MYB proteins, 113 2R-MYB proteins, and 98 1R-MYB proteins. The phylogenetic relationship of each MYB subgroup was consistent in terms of intron/exon structure and conserved motif composition. The MYB genes in woodland strawberry underwent loss and expansion events during evolution. The transcriptome data revealed that most FveMYB genes are expressed in several organs, whereas 15 FveMYB genes exhibit organ-specific expression, including five genes (FveMYB101, -112, -44, and -8; FveMYB1R81) in roots, two genes (FveMYB62 and -77) in stolon tips, three genes (FveMYB99 and -35; FveMYB1R96) in open flowers, and five genes (FveMYB76 and -100; FveMYB1R4, -5, and -86) in immature fruits. During fruit ripening of woodland strawberry, the expression levels of 84 FveMYB genes were decreased, of which five genes (FveMYB4, -22, -50, and -66; FveMYB1R57) decreased more than 10-fold, whereas those 18 FveMYB genes were increased, especially FveMYB10 and FveMYB74 increased more than 30-fold. In addition, the expression levels of 36, 68, 52, and 62 FveMYB genes were altered by gibberellic acid, abscisic acid, cold, and heat treatments, respectively, and among them, several genes exhibited similar expression patterns for multiple treatments, suggesting possible roles in the crosstalk of multiple signaling pathways. This study provides candidate genes for the study of stolon formation, fruit development and ripening, and abiotic stress responses.
Interventions on Food Security and Water Uses for Improving Nutritional Status of Pregnant Women and Children Younger Than Five Years in Low-Middle Income Countries: A Systematic Review
Authors: Urgell-Lahuerta Cristina , Carrillo-Álvarez Elena , Salinas-Roca Blanca
Published: May 2021

Malnutrition is a global health issue concerning children and pregnant women in low- and middle-income countries (LMICs). The aim of this review was to assess the health-impact outcomes of interventions addressing food security, water quality and hygiene in order to address the improvement of the nutritional status in children below five years and pregnant women in LMICs. Using PRISMA procedures, a systematic review was conducted by searching in biomedical databases clinical trials and interventions for children and pregnant women. Full articles were screened (nf = 252) and critically appraised. The review included 27 randomized and non-randomized trials and interventions. Based on the analysis, three agents concerning nutritional status were identified. First, exclusive breastfeeding and complementary feeding were fundamental elements in preventing malnutrition. Second, provision of sanitation facilities and the promotion of hygienic practices were also essential to prevent infections spread and the consequent deterioration of nutritional status. Finally, seasonality was also seen to be a relevant factor to consider while planning and implementing interventions in the populations under study. In spite of the efforts conducted over last decades, the improvement in food insecurity rates has remained insufficient. Therefore, the development of global health programs is fundamental to guide future actions.
Factors Influencing Sleep Quality among Female Staff Nurses during the Early COVID-19 Pandemic in the United States
Authors: Kim-Godwin Yeoun Soo , Lee Meen Hye , Logan Jeongok G. , Liu Xiaoyue
Published: May 2021

This study aimed to assess the overall level of sleep quality among female staff nurses in the United States during the early COVID-19 pandemic. It also aimed to examine factors associated with sleep quality and its seven subcomponents: subjective sleep quality, sleep latency, sleep duration, habitual sleep efficiency, sleep disturbance, use of sleeping medications, and daytime dysfunction. A descriptive, correlational, and cross-sectional study design was used. We performed descriptive, and regression analyses with a sample of 215 female staff nurses enrolled in post-licensure online nursing programs at a southeastern state university. Data collection was conducted using an online survey from April to May 2020. Sleep quality was measured using the Pittsburgh Sleep Quality Index (PSQI). Nurses working part time (p = 0.02), with lower perceived physical health (p = 0.01), a lower self-care self-regulation score (p < 0.001), and higher work stress (p < 0.05) showed poorer sleep quality. Factors associated with subcomponents of sleep quality varied. Poor sleep quality among nurses during the COVID-19 pandemic was reported. Various factors, including work environmental factors were associated with the sleep quality in this sample. Hospital administrators should consider developing intervention programs for improving the work environment, which would impact sleep quality, health status, and job performance.
Adolescent Use and Perceptions of JUUL and Other Pod-Style e-Cigarettes: A Qualitative Study to Inform Prevention
Authors: Wagoner Kimberly G. , King Jessica L. , Alexander Amir , Tripp Hollie L. , Sutfin Erin L.
Published: May 2021

JUUL, a discrete pod-style e-cigarette, popular among adolescents, delivers high levels of nicotine. Limited research has assessed social and environmental influences that contribute to use of JUUL and other pod-style devices. We examined how these factors, as well as individual characteristics, shape adolescent use. Twenty-nine middle and high school students participated in six focus groups in June 2019 (58.6% female, 65.5% White, 27.6% Hispanic). Groups were stratified by e-cigarette use status and grade to understand perceptions and experiences among groups. Transcripts were coded using thematic analysis for individual, social, and environmental factors contributing to use. Users (n = 13) described their first experience with JUUL as mostly negative, mentioning reactions such as burning in the throat, coughing, wheezing, and headaches. Despite a negative first experience, stress relief and addiction were mentioned as reasons for continued use. Users and non-users identified vaping as a source of disruption to their daily life. Social factors included peer and parental influences, lack of support for quitting, and accessibility. Environmental factors included contrasting messages about long- and short-term health effects of e-cigarettes, as well as a lack of school vaping policy enforcement, health education, medical screenings, and cessation resources. Findings highlight the complex social system that influences adolescent e-cigarette use and have important implications for school and community responses. Strategies to prevent or reduce use may include reviewing existing school tobacco policies, providing counseling and cessation resources, training staff, and increasing knowledge through public education campaigns.
Reducing Loneliness in Stationary Geriatric Care with Robots and Virtual Encounters—A Contribution to the COVID-19 Pandemic
Authors: Follmann Andreas , Schollemann Franziska , Arnolds Andrea , Weismann Pauline , Laurentius Thea , Rossaint Rolf , Czaplik Michael
Published: May 2021

The bans on visiting nursing homes during the COVID-19 pandemic, while intended to protect residents, also have the risk of increasing the loneliness and social isolation that already existed among the older generations before the pandemic. To combat loneliness and social isolation in nursing homes, this trial presents a study during which social networks of nursing home residents and elderly hospital patients were maintained through virtual encounters and robots, respectively. The observational trial included volunteers who were either residents of nursing homes or patients in a geriatric hospital. Each volunteer was asked to fill in a questionnaire containing three questions to measure loneliness. The questionnaire also documented whether video telephony via the robot, an alternative contact option (for example, a phone call), or no contact with relatives had taken place. The aim was to work out the general acceptance and the benefits of virtual encounters using robots for different roles (users, relatives, nursing staff, facilities). Seventy volunteers with three possible interventions (non-contact, virtual encounters by means of a robot, and any other contact) took part in this trial. The frequency of use of the robot increased steadily over the course of the study, and it was regularly used in all facilities during the weeks of visitor bans (n = 134 times). In the hospital, loneliness decreased significantly among patients for whom the robot was used to provide contact (F(1,25) = 7.783, p = 0.01). In the nursing homes, no demonstrable effect could be achieved in this way, although the subject feedback from the users was consistently positive.
Development of a Digital Lifestyle Modification Intervention for Use after Transient Ischaemic Attack or Minor Stroke: A Person-Based Approach
Authors: Heron Neil , O’Connor Seán R. , Kee Frank , Thompson David R. , Anderson Neil , Cutting David , Cupples Margaret E. , Donnelly Michael
Published: May 2021

This paper describes the development of the ‘Brain-Fit’ app, a digital secondary prevention intervention designed for use in the early phase after transient ischaemic attack (TIA) or minor stroke. The aim of the study was to explore perceptions on usability and relevance of the app in order to maximise user engagement and sustainability. Using the theory- and evidence-informed person-based approach, initial planning included a scoping review of qualitative evidence to identify barriers and facilitators to use of digital interventions in people with cardiovascular conditions and two focus groups exploring experiences and support needs of people (N = 32) with a history of TIA or minor stroke. The scoping review and focus group data were analysed thematically and findings were used to produce guiding principles, a behavioural analysis and explanatory logic model for the intervention. Optimisation included an additional focus group (N = 12) and individual think-aloud interviews (N = 8) to explore perspectives on content and usability of a prototype app. Overall, thematic analysis highlighted uncertainty about increasing physical activity and concerns that fatigue might limit participation. Realistic goals and progressive increases in activity were seen as important to improving self-confidence and personal control. The app was seen as a useful and flexible resource. Participant feedback from the optimisation phase was used to make modifications to the app to maximise engagement, including simplification of the goal setting and daily data entry sections. Further studies are required to examine efficacy and cost-effectiveness of this novel digital intervention.
Spatial Distribution Characteristics of Heavy Metals in Surface Soil of Xilinguole Coal Mining Area Based on Semivariogram
Authors: Chen Guoqing , Yang Yong , Liu Xinyao , Wang Mingjiu
Published: May 2021

Heavy metal pollution is a major environmental problem facing humankind. Locating the source and distribution of heavy metal pollutants around mines can provide a scientific basis for environmental control. The structure effect and random effect of a semivariogram can be used to determine the reason for spatial differences in the heavy metal content in surface soil, and the coefficient of variation and regression analysis can be used to confirm that the verification accuracy meets the geostatistical requirements. According to the maximum difference method, the content of heavy metals in the surface soil of the mining area is higher than that of the surroundings, and Cu and Zn levels are higher than the background values for Inner Mongolia. In the present case, Zn, Mn, Pb, Cr, Ni, and Cu levels exceeded the background values for the surroundings of the study area by 65.10%, 53.72%, 52.17%, 46.24%, 33.08%, and 29.49%, respectively. The results show that human activities play a decisive role in the spatial distribution of heavy metals, leading to their spatial distribution in the form of “core periphery”. This distribution pattern was significantly affected by the slope, NDVI value, and the distance from the mining area, but the spatial distribution of Pb was significantly related to high-grade roads. The research methods and conclusions have reference significance for the sources and spatial distribution characteristics of heavy metal pollution in similar mining areas and provide a target for the prevention and control of environmental pollution in the study area.
The Effects of Random Porosities in Resonant Frequencies of Graphene Based on the Monte Carlo Stochastic Finite Element Model
Authors: Chu Liu , Shi Jiajia , Yu Yue , Souza De Cursi Eduardo
Published: May 2021

With the distinguished properties in electronics, thermal conductivity, optical transparence and mechanics, graphene has a powerful potential in nanosensors, nano-resonators, supercapacitors, batteries, etc. The resonant frequency of graphene is an important factor in its application and working environment. However, the random dispersed porosities in graphene evidently change the lattice structure and destroy the integrity and geometrical periodicity. This paper focuses on the effects of random porosities in resonant frequencies of graphene. Monte Carlo simulation is applied to propagate the porosities in the finite element model of pristine graphene. The statistical results and probability density distribution of porous graphene with atomic vacancy defects are computed based on the Monte Carlo finite element model. The results of porous graphene with atomic vacancy defects are compared and discussed with the results of graphene with bond vacancy defects. The enhancement effects of atomic vacancy defects are confirmed in porous graphene. The influences of atomic vacancy defects on displacement and rotation vector sums of porous graphene are more concentrated in local places.
Evaluation of the PSMA-Binding Ligand 212Pb-NG001 in Multicellular Tumour Spheroid and Mouse Models of Prostate Cancer
Authors: Stenberg Vilde Yuli , Larsen Roy Hartvig , Ma Li-Wei , Peng Qian , Juzenas Petras , Bruland Øyvind Sverre , Juzeniene Asta
Published: May 2021

Radioligand therapy targeting the prostate-specific membrane antigen (PSMA) is rapidly evolving as a promising treatment for metastatic castration-resistant prostate cancer. The PSMA-targeting ligand p-SCN-Bn-TCMC-PSMA (NG001) labelled with 212Pb efficiently targets PSMA-positive cells in vitro and in vivo. The aim of this preclinical study was to evaluate the therapeutic potential of 212Pb-NG001 in multicellular tumour spheroid and mouse models of prostate cancer. The cytotoxic effect of 212Pb-NG001 was tested in human prostate C4-2 spheroids. Biodistribution at various time points and therapeutic effects of different activities of the radioligand were investigated in male athymic nude mice bearing C4-2 tumours, while long-term toxicity was studied in immunocompetent BALB/c mice. The radioligand induced a selective cytotoxic effect in spheroids at activity concentrations of 3–10 kBq/mL. In mice, the radioligand accumulated rapidly in tumours and was retained over 24 h, while it rapidly cleared from nontargeted tissues. Treatment with 0.25, 0.30 or 0.40 MBq of 212Pb-NG001 significantly inhibited tumour growth and improved median survival with therapeutic indexes of 1.5, 2.3 and 2.7, respectively. In BALB/c mice, no signs of long-term radiation toxicity were observed at activities of 0.05 and 0.33 MBq. The obtained results warrant clinical studies to evaluate the biodistribution, therapeutic efficacy and toxicity of 212Pb-NG001.
In Vivo Analysis of the Biocompatibility and Bone Healing Capacity of a Novel Bone Grafting Material Combined with Hyaluronic Acid
Authors: Pröhl Annica , Batinic Milijana , Alkildani Said , Hahn Michael , Radenkovic Milena , Najman Stevo , Jung Ole , Barbeck Mike
Published: May 2021

The present in vivo study analyses both the inflammatory tissue reactions and the bone healing capacity of a newly developed bone substitute material (BSM) based on xenogeneic bone substitute granules combined with hyaluronate (HY) as a water-binding molecule. The results of the hyaluronate containing bone substitute material (BSM) were compared to a control xenogeneic BSM of the same chemical composition and a sham operation group up to 16 weeks post implantationem. A major focus of the study was to analyze the residual hyaluronate and its effects on the material-dependent healing behavior and the inflammatory tissue responses. The study included 63 male Wistar rats using the calvaria implantation model for 2, 8, and 16 weeks post implantationem. Established and Good Laboratory Practice (GLP)-conforming histological, histopathological, and histomorphometrical analysis methods were conducted. The results showed that the new hyaluronate containing BSM was gradually integrated within newly formed bone up to the end of the study that ended in a condition of complete bone defect healing. Thereby, no differences to the healing capacity of the control BSM were found. However, the bone formation in both groups was continuously significantly higher compared to the sham operation group. Additionally, no differences in the (inflammatory) tissue response that was analyzed via qualitative and (semi-) quantitative methods were found. Interestingly, no differences were found between the numbers of pro- and anti-inflammatory macrophages between the three study groups over the entire course of the study. No signs of the HY as a water-binding part of the BSM were histologically detectable at any of the study time points, altogether the results of the present study show that HY allows for an optimal material-associated bone tissue healing comparable to the control xenogeneic BSM. The added HY seems to be degraded within a very short time period of less than 2 weeks so that the remaining BSM granules allow for a gradual osteoconductive bone regeneration. Additionally, no differences between the inflammatory tissue reactions in both material groups and the sham operation group were found. Thus, the new hyaluronate containing xenogeneic BSM and also the control BSM have been shown to be fully biocompatible without any differences regarding bone regeneration.
Cell Shape and Matrix Stiffness Impact Schwann Cell Plasticity via YAP/TAZ and Rho GTPases
Authors: Xu Zhenyuan , Orkwis Jacob A. , Harris Greg M.
Published: May 2021

Schwann cells (SCs) are a highly plastic cell type capable of undergoing phenotypic changes following injury or disease. SCs are able to upregulate genes associated with nerve regeneration and ultimately achieve functional recovery. During the regeneration process, the extracellular matrix (ECM) and cell morphology play a cooperative, critical role in regulating SCs, and therefore highly impact nerve regeneration outcomes. However, the roles of the ECM and mechanotransduction relating to SC phenotype are largely unknown. Here, we describe the role that matrix stiffness and cell morphology play in SC phenotype specification via known mechanotransducers YAP/TAZ and RhoA. Using engineered microenvironments to precisely control ECM stiffness, cell shape, and cell spreading, we show that ECM stiffness and SC spreading downregulated SC regenerative associated proteins by the activation of RhoA and YAP/TAZ. Additionally, cell elongation promoted a distinct SC regenerative capacity by the upregulation of Rac1/MKK7/JNK, both necessary for the ECM and morphology changes found during nerve regeneration. These results confirm the role of ECM signaling in peripheral nerve regeneration as well as provide insight to the design of future biomaterials and cellular therapies for peripheral nerve regeneration.
Effects of Multi-Component Backgrounds of Volatile Plant Compounds on Moth Pheromone Perception
Authors: Conchou Lucie , Lucas Philippe , Deisig Nina , Demondion Elodie , Renou Michel
Published: May 2021

The volatile plant compounds (VPC) alter pheromone perception by insects but mixture effects inside insect olfactory landscapes are poorly understood. We measured the activity of receptor neurons tuned to Z7-12Ac (Z7-ORN), a pheromone component, in the antenna and central neurons in male Agrotis ipsilon while exposed to simple or composite backgrounds of a panel of VPCs representative of the odorant variety encountered by a moth. Maps of activities were built using calcium imaging to visualize which areas in antennal lobes (AL) were affected by VPCs. We compared the VPC activity and their impact as backgrounds at antenna and AL levels, individually or in blends. At periphery, VPCs showed differences in their capacity to elicit Z7-ORN firing response that cannot be explained by differences in stimulus intensities because we adjusted concentrations according to vapor pressures. The AL neuronal network, which reformats the ORN input, did not improve pheromone salience. We postulate that the AL network evolved to increase sensitivity and to encode for fast changes of pheromone at some cost for signal extraction. Comparing blends to single compounds indicated that a blend shows the activity of its most active component. VPC salience seems to be more important than background complexity.
Clinical Features and Diagnosis of Cardiac Sarcoidosis
Authors: Tana Claudio , Mantini Cesare , Donatiello Iginio , Mucci Luciano , Tana Marco , Ricci Fabrizio , Cipollone Francesco , Giamberardino Maria Adele
Published: May 2021

Cardiac sarcoidosis (CS) is an unusual, but potentially harmful, manifestation of systemic sarcoidosis (SA), a chronic disease characterized by organ involvement from noncaseating and nonnecrotizing granulomas. Lungs and intrathoracic lymph nodes are usually the sites that are most frequently affected, but no organ is spared and CS can affect a variable portion of SA patients, up to 25% from post-mortem studies. The cardiovascular involvement is usually associated with a bad prognosis and is responsible for the major cause of death and complications, particularly in African American patients. Furthermore, the diagnosis is often complicated by the occurrence of non-specific clinical manifestations, which can mimic the effect of more common heart disorders, and imaging and biopsies are the most valid approach to avoid misdiagnosis. This narrative review summarizes the main clinical features of CS and imaging findings, particularly of CMR and 18-Fluorodeoxyglucose Positron Emission Tomography (18F-FDG PET) that can give the best cost/benefit ratio in terms of the diagnostic approach. Imaging can be very useful in replacing the endomyocardial biopsy in selected cases, to avoid unnecessary, and potentially dangerous, invasive maneuvers.
Virtual Therapeutic Garden: A Promising Method Supporting the Treatment of Depressive Symptoms in Late-Life: A Randomized Pilot Study
Authors: Szczepańska-Gieracha Joanna , Cieślik Błażej , Serweta Anna , Klajs Krzysztof
Published: May 2021

The multifactorial genesis of old-age depression requires multi-professional therapy combining physical activity and psychosocial interventions; however, there is still a percentage of older people who do not exhibit satisfactory improvements. The aim of this study was to evaluate the effectiveness of virtual therapy in the elderly for whom the previous multimodal, biopsychosocial therapeutic programme had not brought the expected results. Twenty-five elderly women with depressive symptoms were randomly divided into a virtual reality group (VR, n = 13) and a control group (Control, n = 12). The average age was 70.73 and the average intensity of depression symptoms amounted to 12.26 in the Geriatric Depression Scale (GDS-30). As a virtual reality source, the VRTierOne (Stolgraf®) device was used. The therapeutic cycle consisted of eight virtual therapy sessions, twice a week for four weeks. As primary and secondary outcome measures, the GDS-30 was performed at three time points. In the VR group, the GDS-30 score was reduced by 36%, and the result persisted in the follow-up tests. Immersive virtual therapy significantly lowered the intensity of depressive symptoms, as well as stress and anxiety levels in older women taking part in the group-based multimodal therapeutic programme, whose earlier therapy had not brought the expected results.