bluuebunny/crossref_metadata_2025 huggingface dataset:
Inferior Vestibular Neuritis: Diagnostic Criteria, Clinical Features, and Prognosis—A Focused Review
Authors: Musat Gabriela Cornelia , Preda Mihai Alexandru , Tanase Ionut , Anton Adina Zamfir Chiru , Mitroi George G. , Musat Ovidiu , Oancea Alina Lavinia Antoaneta , Mitroi Mihaela Roxana
Published: February 2025

Purpose: This review aims to analyze the diagnostic methods used to evaluate inferior vestibular nerve neuritis. Methods: We performed an electronic search on the PubMed database for clinical studies investigating the diagnostic techniques used for inferior vestibular nerve neuritis. Results: We initially identified 114 records in our search. After applying the inclusion and exclusion criteria, we narrowed it down to 12 studies. These studies collectively examined a total of 642 patients diagnosed with vestibular nerve neuritis, 64 of whom had inferior vestibular neuritis. Conclusions: The inferior vestibular neuritis is an unfrequent form of vestibular neuritis, often misdiagnosed. The diagnosis of inferior vestibular neuritis (IVN) is based on clinical history and vestibular testing, specifically reduced posterior canal gain on video head impulse test (vHIT), absent cervical VEMP (cVEMP), normal caloric responses, and preserved ocular VEMP (oVEMP). This review highlights the gaps in current diagnostic strategies and emphasizes the need for integrating advanced vestibular testing methods to enhance diagnostic accuracy for inferior vestibular nerve neuritis. Future studies should address the standardization of diagnostic protocols to facilitate broader clinical application.
An Optimized PZT-FBG Voltage/Temperature Sensor
Authors: Sun Shangpeng , Ma Feiyue , He Yanxiao , Niu Bo , Wang Cheng , Dai Longcheng , Zhao Zhongyang
Published: February 2025

The piezoelectric grating voltage sensor has garnered significant attention in the realm of intelligent sensing, attributed to its compact size, cost-effectiveness, robust electromagnetic interference (EMI) immunity, and high network integration capabilities. In this paper, we propose a PZT-FBG (piezoelectric ceramic–fiber Bragg grating) voltage–temperature demodulation optical path architecture. This scheme effectively utilizes the originally unused temperature compensation reference grating, repurposing it as a temperature measurement grating. By employing FBGs with identical or similar parameters, we experimentally validate two distinct optical path connection schemes, before and after optimization. The experimental results reveal that, when the input voltage ranges from 250 V to 1800 V at a frequency of 50 Hz, the goodness of fit for the three fundamental waveforms is 0.996, 0.999, and 0.992, respectively. Furthermore, the sensor’s frequency response was tested across a frequency range of 50 Hz to 20 kHz, demonstrating that the measurement system can effectively respond within the sensor’s operational frequency range. Additionally, temperature measurement experiments showed a goodness of fit of 0.997 for the central wavelength of the FBG as the temperature increased. This research indicates that the improved optical path connection method not only accomplishes a synchronous demodulation of both temperature and voltage parameters but also markedly enhances the linearity and resolution of the voltage sensor. This discovery offers novel insights for further refining sensor performance and broadening the applications of optical voltage sensors.
Microbial Community Composition of Explosive-Contaminated Soils: A Metataxonomic Analysis
Authors: Flores Francisco J. , Mena Esteban , Granda Silvana , Duchicela Jéssica
Published: February 2025

Munition disposal practices have significant effects on microbial composition and overall soil health. Explosive soil contamination can disrupt microbial communities, leading to microbial abundance and richness changes. This study investigates the microbial diversity of soils and roots from sites with a history of ammunition disposal, aiming to identify organisms that may play a role in bioremediation. Soil and root samples were collected from two types of ammunition disposal (through open burning and open detonation) and unpolluted sites in Machachi, Ecuador, over two years (2022 and 2023). High-throughput sequencing of the 16S rRNA gene (for bacteria) and the ITS region (for fungi and plants) was conducted to obtain taxonomic profiles. There were significant variations in the composition of bacteria, fungi, and plant communities between polluted and unpolluted sites. Bacterial genera such as Pseudarthrobacter, Pseudomonas, and Rhizobium were more abundant in roots, while Candidatus Udaeobacter dominated unpolluted soils. Fungal classes Dothideomycetes and Sordariomycetes were prevalent across most samples, while Leotiomycetes and Agaricomycetes were also highly abundant in unpolluted samples. Plant-associated reads showed a higher abundance of Poa and Trifolium in root samples, particularly at contaminated sites, and Alchemilla, Vaccinium, and Hypericum were abundant in unpolluted sites. Alpha diversity analysis indicated that bacterial diversity was significantly higher in unpolluted root and soil samples, whereas fungal diversity was not significantly different among sites. Redundancy analysis of beta diversity showed that site, year, and sample type significantly influenced microbial community structure, with the site being the most influential factor. Differentially abundant microbial taxa, including bacteria such as Pseudarthrobacter and fungi such as Paraleptosphaeria and Talaromyces, may contribute to natural attenuation processes in explosive-contaminated soils. This research highlights the potential of certain microbial taxa to restore environments contaminated by explosives.
Vertical Stratification Reduces Microbial Network Complexity and Disrupts Nitrogen Balance in Seasonally Frozen Ground at Qinghai Lake in Tibet
Authors: Zhang Ni , Zhou Zhiyun , Wang Yijun , Zhou Shijia , Ma Jing , Sun Jianqing , Chen Kelong
Published: February 2025

Global climate change has accelerated the reduction of permafrost regions across different altitude gradients, shortening the duration of the freezing period to varying extents. However, the response of the soil microorganisms of frozen soils along altitude gradients remains unclear. In this study, we employed 16S rRNA sequencing and LC-MS metabolomics to investigate the response of soil microbial communities and soil metabolites to vertical stratification in the permafrost soils of the Qinghai Lake region. The results indicated that Proteobacteria, Firmicutes, and Actinobacteria were key soil bacterial phyla in the permafrost soils of Qinghai Lake during the freezing period, with Proteobacteria and Firmicutes showing significant sensitivity to vertical stratification (p < 0.05). The majority of the physicochemical factors exhibited a trend of initially increasing and then decreasing with increasing altitude, whereas pH showed the opposite trend. pH and moisture content were identified as the most important environmental factors influencing soil bacterial community structure. Deterministic processes dominated the assembly of bacterial communities of frozen soils in the Qinghai Lake basin. Co-occurrence network analysis showed that increasing altitude gradients led to a higher average degree of the bacterial network, while reducing network complexity and inter-species connectivity. Soil metabolomics analysis revealed that vertical stratification altered the metabolic profiles of 27 metabolites, with the significantly changed metabolites primarily associated with carbohydrate and amino acid metabolism. In conclusion, the characteristics of the Qinghai Lake permafrost were regulated by regional vertical stratification, which further influenced microbial community structure and soil metabolic characteristics, thereby altering carbon and nitrogen stocks. Specifically, higher altitudes were more favorable for the retention of the carbon and nitrogen stocks of frozen soils in the Qinghai Lake basin.
The Impact of Human Salivary Amylase Gene Copy Number and Starch on Oral Biofilms
Authors: Superdock Dorothy K. , Johnson Lynn M. , Ren Jennifer , Khan Alizeh , Eno Megan , Man Shuai , Poole Angela C.
Published: February 2025

The copy number (CN) variant AMY1 encodes the salivary amylase enzyme which promotes starch digestion. Although this gene has been associated with dental caries and periodontal disease susceptibility, the impact of the interaction between AMY1 CN and starch on oral biofilms is unclear. We explored how oral microbiota communities shaped by AMY1 CN respond to starch by employing an in vitro model of biofilm formation. We cultured biofilms using saliva samples from 31 donors with a range of AMY1 CNs (between 2 and 20 copies) and self-reported gum disease states; we used media with and without starch. Many of the most prevalent genera in saliva were also prevalent in the derived biofilms. The presence of starch in the media was associated with lower biofilm alpha diversity. We found a significant interaction between AMY1 CN and the media carbohydrate content that influenced the proportions of Atopobium and Veillonella. Members of these genera have been associated with dental caries and periodontitis. These findings suggest that the effects of carbohydrates on oral microbiome composition depend on AMY1 CN and that human oral bacteria evolved in response to expansion of this host gene locus.
Geochemical Insights and Mineral Resource Potential of Rare Earth Elements (REE) in the Croatian Karst Bauxites
Authors: Kovačević Galović Erli , Ilijanić Nikolina , Gizdavec Nikola , Miko Slobodan , Peh Zoran
Published: February 2025

Karst bauxites are valuable terrestrial records of paleoclimate and tectonic evolution formed under tropical to subtropical conditions during the subaerial exposure of carbonate platforms. This study explores Croatian bauxite deposits within the Adriatic–Dinaric Carbonate Platform (ADCP), with a focus on the distribution and enrichment of rare earth elements (REE) across eight bauxite horizons from the Triassic to Neogene periods. The research applies statistical analyses of geochemical data, as well as developed models, to assess the factors influencing REE distribution and fractionation. The study found that variations in parent material, along with changes in paleogeographical and paleotectonic settings, significantly affected the REE content. The median REE concentrations in the analyzed bauxite horizons range from approximately 250 to 570 mg/kg. Notable REE enrichment was observed in the Late Paleogene, particularly in the Middle and Upper Eocene horizons. The analysis highlights the importance of physicochemical conditions, such as Eh and pH, during the weathering processes that lead to bauxite formation. The results suggest that the presence of REE-bearing minerals, rather than clay minerals, could possibly contribute to elevated concentrations of heavy REE (HREE). These findings indicate that Croatian bauxites, enriched in REE and associated trace elements, are significant not only as geological markers of past climatic and tectonic events but also as potential sources of critical raw materials. This study underscores the potential for the economic exploitation of these deposits in the context of modern technological demands.
Natural Polyamine Spermidine Inhibits the In Vitro Oxidation of LDL
Authors: Rossmann Christine , Darko Azra , Kager Gerd , Ledinski Gerhard , Wonisch Willibald , Wagner Thomas , Hallström Seth , Reibnegger Gilbert , Paar Margret , Cvirn Gerhard
Published: February 2025

Spermidine is a natural autophagy-inducer and anti-aging compound. Herein, we investigated a potential autophagy-independent mechanism of spermidine, namely its capability to directly impede LDL oxidation, an early step in atherogenesis. In our in vitro-model, LDL oxidation was induced by the addition of CuCl2 in the presence of increasing concentrations of spermidine, and the degree of oxidation of the lipid, as well as of the protein part of LDL, was measured. We found that spermidine concentration-dependently inhibited the production of lipid hydroperoxides, malondialdehyde, and oxidation-specific immune epitopes in the LDL particle, associated with decreased relative electrophoretic mobilities, respectively. For example, the LPO content was significantly lower when LDL was oxidized in the presence of 500 µg/mL spermidine (26.9 ± 1.6 nmol/mg LDL) than in the absence of spermidine (180.6 ± 7.7 nmol/mg LDL, p < 0.0001). When oxLDL was obtained under increasing spermidine concentrations, its cytotoxicity in EA.hy926 cells concentration-dependently decreased. Quantum chemical calculations show that the reaction between spermidine and hydroxyl radicals is exergonic. We conclude that spermidine is a direct inhibitor of LDL oxidation due to its capability to scavenge hydroxyl radicals. Thus, spermidine supplementation might be a suitable tool to impede atherogenesis and associated (cardio)vascular diseases. Further prospective clinical studies are needed to evaluate the potential atheroprotective/health-promoting effects of spermidine-rich diets.
Revisiting the Mechanistic Pathway of Gas-Phase Reactions in InN MOVPE Through DFT Calculations
Authors: He Xiaokun , Xu Nan , Xue Yuan , Zhang Hong , Zuo Ran , Xu Qian
Published: February 2025

III-nitrides are crucial materials for solar flow batteries due to their versatile properties. In contrast to the well-studied MOVPE reaction mechanism for AlN and GaN, few works report gas-phase mechanistic studies on the growth of InN. To better understand the reaction thermodynamics, this work revisited the gas-phase reactions involved in metal–organic vapor-phase epitaxy (abbreviated as MOVPE) growth of InN. Utilizing the M06-2X function in conjunction with Pople’s triple-ζ split-valence basis set with polarization functions, this work recharacterized all stationary points reported in previous literature and compared the differences between the structures and reaction energies. For the reaction pathways which do not include a transition state, rigorous constrained geometry optimizations were utilized to scan the PES connecting the reactants and products in adduct formation and XMIn (M, D, T) pyrolysis, confirming that there are no TSs in these pathways, which is in agreement with the previous findings. A comprehensive bonding analysis indicates that in TMIn:NH3, the In-N demonstrates strong coordinate bond characteristics, whereas in DMIn:NH3 and MMIn:NH3, the interactions between the Lewis acid and base fragments lean toward electrostatic attraction. Additionally, the NBO computations show that the H radical can facilitate the migration of electrons that are originally distributed between the In-C bonds in XMIn. Based on this finding, novel reaction pathways were also investigated. When the H radical approaches MMInNH2, MMIn:NH3 rather than MMInHNH2 will generate and this is followed by the elimination of CH4 via two parallel paths. Considering the abundance of H2 in the environment, this work also examines the reactions between H2 and XMIn. The Mulliken charge distributions indicated that intermolecular electron transfer mainly occurs between the In atom and N atom whiling forming (DMInNH2)2, whereas it predominately occurs between the In atom and the N atom intramolecularly when generating (DMInNH2)3.
The Puzzle of the Regioselectivity and Molecular Mechanism of the (3+2) Cycloaddition Reaction Between E-2-(Trimethylsilyl)-1-Nitroethene and Arylonitrile N-Oxides: Molecular Electron Density Theory (MEDT) Quantumchemical Study
Authors: Sadowski Mikołaj , Dresler Ewa , Jasiński Radomir
Published: February 2025

The regioselectivity and molecular mechanism of the (3+2) cycloaddition reaction between E-2-(trimethylsilyl)-1-nitroethene and arylonitrile N-oxides were explored on the basis of the ωB97XD/6-311+G(d) (PCM) quantumchemical calculations. It was found that the earlier postulate regarding the regioselectivity of the cycloaddition stage should be undermined. Within our research, several aspects of the title reaction were also examined: interactions between reagents, electronic structures of alkenes and nitrile oxides, the nature of transition states, the influence of the polarity solvent on the reaction selectivity and mechanism, substituent effects, etc. The obtained results offer a general conclusion for all of the important aspects of some groups of cycloaddition processes.
Review of Biomass-Derived Carbon Nanomaterials—From 0D to 3D—For Supercapacitor Applications
Authors: Yan Yihong , Sun Weiqiang , Wei Yuxin , Liu Kuankuan , Ma Jingjing , Hu Guang
Published: February 2025

The transition to sustainable energy storage solutions has driven significant interest in supercapacitors, which offer high power density, rapid charge–discharge capabilities, and exceptional cycle stability. Biomass-derived carbon nanomaterials have emerged as compelling candidates for supercapacitor electrodes due to their renewable origins, environmental compatibility, and cost-effectiveness. This study explores recent advancements in tailoring structural properties, for example in preparation methods and activation, which are essential for efficient charge storage and rapid ion transport. Attention is given to the dimensional configurations—spanning 0D to 3D structures—and their impact on electrochemical behaviors. This review outlines the challenges faced in scaling up and optimizing these materials for practical applications, alongside an outlook on future research directions. By bridging the gap between material design and application demands, this work contributes to advancing sustainable supercapacitor technologies for a greener energy future.
A Comparison Between Ripening Under a Constant Volume and Ripening Under a Constant Surface Area
Authors: Tu King-Ning , Gusak Andriy M. , Sun Qinglei , Yao Yifan
Published: February 2025

The classic Lifshitz–Slyozov–Wagner (LSW) theory of ripening assumes a constant volume. In comparison, we present here a model of ripening assuming a constant surface area, which has occurred in the microstructure changes in intermetallic compounds in micro-bump for 3D integrated-circuit (IC) technology in consumer electronic products. However, to keep a constant surface area requires the growth of the volume. Furthermore, in 3D IC technology, the kinetics is affected by electrical charges flowing in and out of the system. Due to Joule heating and electromigration, heat flux and atomic flux can occur together. The kinetic modes of failure changes are given here, as well as the mean-time-to-failure equations based on entropy production.
Dietary Protein Intake Is a Determining Factor for Skeletal Muscle Mass in Japanese Older People with Type 2 Diabetes: A Cross-Sectional Study
Authors: Moyama Shota , Yamazaki Yuji , Takahashi Takuya , Makabe Noboru , Hamamoto Yoshiyuki , Kurose Takeshi , Yamada Yuichiro , Kuwata Hitoshi , Seino Yutaka
Published: February 2025

Background/Objectives: In this study, we investigated the free-living nutritional intake of older people with type 2 diabetes (T2D) and examined the relationship between nutritional intake and skeletal muscle mass. Methods: Subjects aged 65 years or older with T2D who visited the Kansai Electric Power Hospital between 2015 and 2017 and had not yet received nutritional guidance or intervention at our hospital comprised the study group. Nutritional intake (energy, protein, lipid, and carbohydrate intake) was calculated from a 3-day dietary diary by the participants, and the relationship between nutritional intake and the skeletal muscle index (SMI) was retrospectively investigated. Results: In total, 91 subjects were recruited (53 males and 38 females, aged 70.3 ± 5.5 years). The energy and protein intakes were 28.7 ± 6.1 kcal/kg/day and 1.2 ± 0.3 g/kg/day, respectively. A significant positive correlation was found between the SMI and energy and protein intake (p < 0.001). Multiple regression analysis with the SMI as the dependent variable and age, gender, protein intake, lipid intake, and carbohydrate intake as the independent variables revealed protein intake to be an independent determinant of the SMI. Conclusions: In older people with T2D, the energy and protein intakes are likely to be lower than the recommended levels. Appropriate interventions for protein intake and energy intake are recommended to prevent loss of muscle mass in Japanese older people with type 2 diabetes.
Moderate Highland Barley Intake Affects Anti-Fatigue Capacity in Mice via Metabolism, Anti-Oxidative Effects and Gut Microbiota
Authors: Zhao Liangxing , Zhao Qingyu , Sharafeldin Sameh , Sang Luman , Wang Chao , Xue Yong , Shen Qun
Published: February 2025

Objectives: this study aimed to explore the effects of different intake levels (20–80%) of highland barley on the anti-fatigue capacity of ICR mice, focusing on energy metabolism, metabolite accumulation, oxidative stress, and changes in the gut microbiota. Methods: male ICR mice were assigned to five groups: control (normal diet) and four experimental groups with highland barley supplementation at 20%, 40%, 60%, and 80% of total dietary energy. Anti-fatigue performance was assessed by behavioral experiments (rotarod, running, and exhaustive swimming tests), biochemical markers, and gut microbiota analysis. Results: the results showed that moderate supplementation (20%) significantly enhanced exercise endurance and anti-fatigue capacity, as evidenced by increased liver glycogen (134.48%), muscle glycogen (87.75%), ATP content (92.07%), Na+-K+-ATPase activity (48.39%), and antioxidant enzyme activities (superoxide dismutase (103.31%), catalase (87.75%), glutathione peroxidase (81.14%). Post-exercise accumulation of blood lactate, quadriceps muscle lactate, serum urea nitrogen, and the oxidative stress marker malondialdehyde was significantly reduced, with differences of 31.52%, 21.83%, 21.72%, and 33.76%, respectively. Additionally, 20% supplementation promoted the growth of beneficial gut microbiota associated with anti-fatigue effects, including unclassified_f_Lachnospiraceae, g_norank_f_Peptococcaceae, Lachnospiraceae NK4A136, Colidextribacter, and Turicibacter. However, when intake reached 60% or more, anti-fatigue effects diminished, with decreased antioxidant enzyme activity, increased accumulation of metabolic waste, and a rise in potentially harmful microbiota (Allobaculum, Desulfovibrio, and norank_f_norank_o_RF39). Conclusions: moderate highland barley supplementation (20% of total dietary energy) enhances anti-fatigue capacity, while excessive intake (≥60%) may have adverse effects.
Fatal Toxoplasmosis in Red Kangaroos (Macropus rufus) in East China
Authors: Gong Haiyan , Wang Quan , Jin Yinghong , Qiu Suoping , Chen Zhaoguo , Han Xiangan , Chen Zongyan , Jiang Wei
Published: February 2025

Background: As a ubiquitous apicomplexan parasite, Toxoplasma gondii causes huge economic losses and poses a great threat to the health of animals, including humans, worldwide. In some kangaroo species, T. gondii can be fatal. To date, little information is available on T. gondii infection in the red kangaroos in east China. At a zoo in east China, thirteen red kangaroos consecutively developed clinical signs from July to November 2016, resulting in the deaths of seven, three of which were analyzed in this study. Methods: In the present study, ascitic fluid, blood and samples from the brain, heart, liver, spleen, lung, kidney, and mesenteric lymph nodes of three dead red kangaroos were collected. The pathogen was explored through microscopic observation, nested PCR, immunofluorescence antibody test (IFAT), hematoxylin–eosin (HE) staining, and immunohistochemistry (IHC) staining, respectively. Meanwhile, the potential source of the infection was also investigated by testing the blood of stray cats in the zoo for T. gondii using nested PCR. Results: Three dead red kangaroos were subjected to a necropsy, and organisms resembling T. gondii were detected in their ascitic fluids under microscope. This infection was further confirmed by a nested PCR assay, which resulted in a successful amplification and sequencing of the 433 bp fragment of the T. gondii 5.8S rRNA gene in all the dissected tissues, including heart, liver, spleen, lung, kidney, lymph nodes, cecum, and brain, as well as in body fluids (blood and ascitic fluid). Furthermore, the tachyzoites were observed in the heart, liver, spleen, lymph nodes, cecum, and brain through IFAT and HE staining. Administration of classic drugs (sulfadiazine and pyrimethamine) against T. gondii significantly alleviated the clinical signs of the sick kangaroos. The possible source of this infection was traced to a native stray cat, as T. gondii DNA was detected in its blood. Conclusions: In the present study, lethal T. gondii infection in red kangaroos has been described for the first time in east China, highlighting the necessity and urgency for close and long-term surveillance of this parasite infection in captive animals. The same strain of T. gondii detected in kangaroos as that found in stray cats wandering in the same area emphasizes the importance of controlling stray cat populations to mitigate the risk of Toxoplasma transmission to other animals.
First Molecular Detection of Rickettsia conorii and Rickettsia helvetica in Ticks from Dogs in Luxembourg
Authors: Moreira Guilherme , Moreira Rafaela S. S. , Neves Floriane André das , Swiontek Vanessa , Barradas Patrícia F. , Gomes-Gonçalves Sara , Mesquita João R.
Published: February 2025

Vector-borne diseases, particularly those caused by Rickettsia species, pose a significant public health threat in Europe. Despite extensive research on tick-borne pathogens in various European countries, Luxembourg has yet not been studied for Rickettsia spp. in ticks infesting domestic animals. This study aimed to fill this gap by investigating the presence of Rickettsia spp. in Ixodes ricinus ticks collected from domestic dogs in Luxembourg between April 2023 and April 2024. A total of 61 ticks were examined using molecular techniques, including PCR amplification of the outer membrane protein B (ompB), outer membrane protein A (ompA), and citrate synthase (gltA) genes. Results revealed the presence of R. helvetica and R. conorii subsp. raoultii, with 4.9% of ticks testing positive for Rickettsia spp. Phylogenetic analysis confirmed the high genetic identity of the sequences obtained with previously described strains from Europe and Asia. This study highlights the potential risk of emerging tick-borne diseases in Luxembourg and emphasizes the need for ongoing surveillance to better understand the spread of Rickettsia spp. in Europe, particularly as climate change may facilitate the expansion of tick populations and their associated pathogens.
Paeoniflorin Attenuates Limb Ischemia by Promoting Angiogenesis Through ERα/ROCK-2 Pathway
Authors: Li Mengyao , Wang Qianyi , Zhu Sinan , Sun Wei , Ren Xiuyun , Xu Zhenkun , Li Xinze , Wang Shaoxia , Liu Qi , Chen Lu , Wang Hong
Published: February 2025

Background: Peripheral artery disease (PAD) is a high-risk vascular condition, and vascular remodeling has become a promising therapeutic approach. Paeoniflorin (PF) is the main bioactive compound in the roots of Paeonia lactiflora Pall, which is commonly used to treat a range of cardiovascular disorders. However, the mechanisms underlying the ameliorating effects of PF on PAD remain unclear. Therefore, the purpose of this study was to explore the therapeutic efficiency of PF on PAD and determine its mechanisms. Methods: The blood flow of mice was detected with a laser Doppler dot scanning imaging system. HE staining was used to observe the morphological changes of ischemic muscle. The changes in the serologic indexes were detected with an automatic biochemical assay, and the capillary density of ischemic gastrocnemius was detected with a Lectin immunofluorescence assay. The expression of angiogenesis-related proteins in ischemic gastrocnemius was detected with Western blotting, and the proportion of macrophages and neutrophils in total cells was detected with flow cytometry. Results: PF significantly increased blood flow, capillary density and protein expressions of vascular endothelial growth factor A (VEGFA), matrix metalloproteinase 2 (MMP2), matrix metalloproteinase 2 (MMP9), and estrogen receptor α (ERα) in mouse ischemic tissue in a PAD model. PF enhances the migration of endothelial cells and promotes the formation of tubular structures, involving the ERα/ROCK2 signaling pathway. Furthermore, PF was found to promote the phenotypic transformation of macrophages and alleviated grave inflammatory responses during vascular remodeling. Conclusions: We determined that PF as a potent compound in promoting angiogenesis and mitigating inflammatory responses during revascularization.
Identification of Furin Protease Small-Molecule Inhibitor with a 1,3-Thiazol-2-ylaminosulfonyl Scaffold
Authors: Kolarič Anja , Ravnik Vid , Štumpf Horvat Sara , Jukič Marko , Bren Urban
Published: February 2025

Background: Proteolytic cleavage of inactive pathogen proteins by furin is critical for their entry into human cells, and thus furin cleavage of the SARS-CoV-2 spike protein was identified as a prerequisite for virus binding and the subsequent infection of human cells in the recent COVID-19 pandemic. We report a water-aware structure-based protease inhibitor design study. Methods: Our efforts focused on the biological evaluation of small molecule inhibitors that emerged from a conserved water-aware virtual screening campaign of a library of compounds that shared structural or physicochemical properties with known furin inhibitors exhibiting newly recognized binding modes. Results: We identified a novel small-molecule furin protease inhibitor with a 1,3-thiazol-2-ylaminosulfonyl scaffold. Namely, the compound N-[4-(1,3-thiazol-2-ylaminosulfonyl)phenyl]-3-{(E)-5-[(2-methoxyphenyl)methylene]-4-oxo-2-thioxo-1,3-thiazolidin-3-yl}propionamide showed an IC50 value of 17.58 μM, comparable to other published inhibitors. Conclusions: This compound could represent a starting point for the further design and development of non-peptidic, small-molecule furin inhibitors that could assist in furin cleavage studies and coronaviral pathogenesis.
Aframomum melegueta Seed Extract’s Effects on Anxiety, Stress, Mood, and Sleep: A Randomized, Double-Blind, Pilot Clinical Trial
Authors: Pérez-Machín Rubén , Vega-Morales Tanausú , Elvira-Aranda Carlos , Lledó-Rico Loreto , Gomis-Gomis María José , López-Ríos Laura
Published: February 2025

Background and aims: Aframomum melegueta (A. melegueta) from the ginger family is appreciated for its pungent seeds widely used in African ethno-medicine. Among the several biological activities associated with the seed’s preparations, some preclinical studies suggest a set of neuroactive properties that have not been tested in humans to date. We performed a clinical trial to investigate the effects of A. melegueta seed extracts on anxiety, stress, mood, and sleep in healthy subjects with moderate anxiety levels. In vitro pharmacological assays targeting the endocannabinoid, serotoninergic, and GABAergic systems were conducted to elucidate the underlying mechanism of action. Methods: A. melegueta standardized to 10% total vanilloids (primarily 6-gingerol, 6-shogaol, and 6-paradol) was obtained after hydroalcoholic extraction and the spray-drying microencapsulation process. Subjects consumed 50, 100, or 150 mg of the extract daily for two days. A set of validated psychometric test questionnaires was collected before and 48 h after the first intake. A. melegueta extract interaction with canonical endocannabinoid receptors (hCB1R and hCB2R), the serotonin receptor (5HT1AR) and gamma-aminobutyric acid receptor (GABAA1R) was evaluated by the radioligand binding assay. Additionally, receptor functional assays and enzyme inhibition assays were conducted to test the extract’s functional activity on the non-canonical endocannabinoid receptor (TRPV1) and the cannabinoid fatty-acid amide hydrolase enzyme (FAAH), respectively. Results: In vitro pharmacological tests showed that the A. melegueta extract activated TRPV1, modulated both hCB2R and 5HT1AR and inhibited FAAH, which is the enzyme primarily responsible for hydrolyzing endogenous anandamide. After a 48 h intake period, the extract significantly reduced anxiety and tension related to stress, improved overall mood, and enhanced sleep quality in the participants at doses ranging from 50 to 150 mg, with no reported side effects. Conclusions: This study supports the potential of the A. melegueta extract for anxiety reduction, mood improvement, stress mitigation, and sleep enhancement. The in vitro tests suggest that the extract’s primary mechanism of action may involve the inhibition of FAAH, which is a key target in anxiety management.
Peanut Genotypes with Reduced Content of Immunogenic Proteins by Breeding, Biotechnology, and Management: Prospects and Challenges
Authors: Alam Tariq , Rustgi Sachin
Published: February 2025

Peanut allergies affect millions of people worldwide, often causing life-threatening reactions and necessitating strict avoidance. Recent advancements in oral immunotherapy, such as Palforzia™, and IgE-mediated treatments (e.g., Xolair), have improved care options; however, their high costs limit accessibility and widespread utility. To address these challenges, researchers are employing conventional breeding and advanced molecular tools, such as CRISPR editing, to develop peanut lines with reduced levels of major allergenic proteins (Ara h1, Ara h2, Ara h3, and Ara h6). These reduced-immunogenicity genotypes retain their agronomic viability, flavor, and nutritional quality to some extent, offering the potential for cost-effective oral immunotherapy and safe food options for use in public spaces by non-allergic individuals. Rigorous evaluation, including immunological assays and human feeding trials, is essential to confirm their effectiveness in reducing allergic reactions. Adoption will depend on the establishment of clear regulatory guidelines, stakeholder education, and transparent communication of the benefits and risks. With sustained research, public trust, and supportive policies, reduced-immunogenicity peanuts could substantially lower the global burden of peanut allergies. This communication examined the impact of peanut allergies worldwide and explored strategies to develop peanut genotypes with reduced allergen content, including conventional breeding and advanced genetic engineering. It also addressed the challenges associated with these approaches, such as policy and regulatory hurdles, and outlined key requirements for their successful adoption by farmers and consumers.
Advanced Nanobiocomposite Hydrogels Incorporating Organofunctionalized LDH for Soft Tissue Engineering Applications
Authors: Radu Ionut-Cristian , Tanasa Eugenia , Dinescu Sorina , Vlasceanu George , Zaharia Catalin
Published: February 2025

Nanocomposite hydrogels are gaining significant attention for biomedical applications in soft tissue engineering due to the increasing demand for highly flexible and durable soft polymer materials. This research paper focused on investigating and optimizing a procedure for the development of novel nanocomposite hydrogels based on poly(2-hydroxyethyl methacrylate)-co-(2-acrylamido-2-methylpropane sulfonic acid) (HEMA/AMPSA) copolymers. These hydrogels were synthesized through a grafting-through process, where the polymer network was formed using a modified clay crosslinker. The layered double hydroxide (LDH) clay modified with 3-(trimethoxysilyl)propyl methacrylate (ATPM) was synthesized using a novel recipe through a two-step procedure. The nanocomposite hydrogel compositions were optimized to achieve soft hydrogels with high flexibility. The developed materials were analyzed for their mechanical and morphological properties using tensile and compressive tests, transmission electron microscopy (TEM), scanning electron microscopy (SEM), and micro-computed tomography (micro-CT). The swelling behavior, network density, and kinetic diffusion mechanism demonstrated the specific characteristics of the materials. The modified LDH-ATPM was further characterized using Thermogravimetry (TGA), FTIR-ATR and X-ray diffraction (XRD). Biological assessments on human adipose-derived stem cells (hASCs) were essential to evaluate the biocompatibility of the nanocomposite hydrogels and their potential for soft tissue applications.
Study on the Impact of Diluent Dosages on the Epoxy–Polythiol Self-Healing System
Authors: Sheng Jiajia , Guo Yang , Pang Xin , Ma Wenjing , Yang Hailu , Liu Yalin , Wang Linbing , Song Shanglin
Published: February 2025

Self-healing technology is an effective method for enhancing the crack resistance of cement-based composites. This study focuses on the impact of the environmentally friendly diluent C12-14 alkyl glycidyl ether (AGE) on the performance of the epoxy resin–polythiol (rimethylolpropane tris (3-mercaptopropionate), TMPMP) self-healing system, examining core fluidity, microcapsule properties, molecular dynamics, and the mechanical properties of cured products. The results show that as the AGE dosage increases, the particle size distribution of microcapsules becomes more concentrated, and the dispersion of particles is improved. Fourier-transform infrared spectroscopy confirms the successful encapsulation of E-51 and AGE. Microcapsules maintain structural integrity at high temperatures of 423.15 K. The onset thermal degradation temperature of the mixture shows an increasing trend with reduced AGE dosage. Specifically, TMPMP35% exhibits an onset degradation temperature of 370.95 K, while that of TMPMP20% is increased by 57.57% compared to TMPMP35%. Conversely, the initial and peak temperatures of the curing reaction decrease with less AGE incorporation. Thermodynamic analysis reveals that activation energy (E) initially increases and then decreases with increasing AGE. The frequency factor (A) correlates positively with the heating rate, indicating that the curing reaction’s reactivity is closely linked to heating rate. Minor variations in the reaction rate constant (k) indicate that the self-healing system maintains stable reactive activity at low temperatures. Notably, the AGE dosage significantly affects the rigidity of the self-healing system; the average Young’s modulus inversely correlates with AGE dosage, with the most substantial decrease of 5.88% occurring when AGE increases from 30% to 35%. This study offers insights into optimizing diluent ratios to balance self-healing and mechanical properties, essential for developing high-performance self-healing cement materials.
Research on the Phenomenological Constitutive Relationship Model of Silicone Structural Adhesives for Glass Curtain Walls
Authors: Jin Nan , Zhao Jianchao , Liang Yuzhu , Zhao Xiaoqing
Published: February 2025

Silicone structural adhesives (SSAs) play a critical role in load transfer within glass curtain wall systems. With the increasing service life of existing glass curtain walls in recent years, their structural safety has become a significant concern across various societal sectors. Accurate characterization of the stress–strain relationship of SSAs is fundamental for evaluating the safety and performance of curtain wall structures. While most existing studies rely on hyperelastic constitutive models derived from rubber materials, employing parameter fitting to describe the constitutive behavior of SSAs, there remains a notable lack of research directly addressing constitutive models tailored specifically for SSAs. Although SSAs share similar chemical compositions with rubber after curing, their mechanical properties exhibit substantial differences. As a result, conventional hyperelastic constitutive models often fail to adequately capture the diverse mechanical responses of SSAs. To address this gap, this study begins with a comprehensive market survey to identify and select representative SSAs. Tensile and shear mechanical experiments are then conducted, with the stress–strain relationships during loading processes accurately captured using advanced techniques, such as Digital Image Correlation (DIC). Building on the Acoustic Emission (AE) algorithm, an intelligent algorithm is developed to optimize the fitting of hyperelastic constitutive parameters, enabling a critical evaluation of the applicability of existing phenomenological hyperelastic models to SSAs. Furthermore, in response to the limitations of current models, a novel and universally applicable constitutive model for SSAs is proposed. The robustness of this model is rigorously validated through comparative analysis with experimental data. The findings of this study provide a universal phenomenological constitutive model for SSAs, significantly enhancing the efficiency and accuracy of constitutive relationship fitting for these materials. This advancement contributes to the improved design, assessment, and maintenance of glass curtain wall systems.
Fabrication and Characterization of Buforin I-Loaded Electrospun Chitosan/Polyethylene Oxide Nanofibrous Membranes with Antimicrobial Activity for Food Packing Applications
Authors: Roshanak Sahar , Yarabbi Hanieh , Movaffagh Jebraeil , Shahidi Fakhri
Published: February 2025

The rising resistance of bacteria to antibiotics has driven the search for new antimicrobial agents. This study focused on encapsulating Buforin I, an antimicrobial peptide, in chitosan/polyethylene oxide (CS-PEO) nanofibers. Buforin I was loaded at a minimum bactericidal concentration (MBC), 10× MBC, and 20× MBC, with assessments on morphology, thermal properties, chemical bonds, crystalline structure, mechanical strength, antimicrobial activity, and cell toxicity. Techniques like differential scanning calorimetry and Fourier-transform infrared spectroscopy confirmed the effective loading of Buforin I in the nanofibers. Scanning electron microscopy showed that Buforin incorporation increased nanofiber diameters. The tensile strength peaked at 20× MBC. Microbial tests indicated that the inhibition zone for nanofibers at 20× MBC surpassed that of commercial antibiotics. Beef coated with CS-PEO nanofibers containing Buforin I demonstrated reduced pH and water activity, alongside lower weight loss during storage. Texture and color analyses revealed that the Buforin I nanofibers helped maintain beef hardness and slowed color degradation compared to control samples. Moreover, thiobarbituric acid levels and total microbial counts in the coated beef were significantly lower than controls (below 3 log CFU/g after 9 days at 4 °C). Thus, these nanofibers may serve as effective antimicrobial packaging agents to delay food spoilage.
Design and Performance Testing Analysis of Underground Electromagnetic Coupling Electro-Hydraulic Signal Wet Joint Scheme
Authors: Wen Min , Xie Renjun , Qiu Hao , Cao Yanfeng , Hou Zening , Qi Zhiyuan , Pan Hao , Huang Hui , Bi Gang
Published: February 2025

The electro-hydraulic composite intelligent completion technology is one of the most effective ways to solve the efficient development of oil and gas. The development of an electro-hydraulic composite wet joint tool that is compatible with the electro-hydraulic composite intelligent completion system can achieve intelligent control between the upper and lower pipe columns of deepwater oil and gas wells and the pluggable transmission of monitoring signals. This article proposes a new type of electromagnetic coupling electro-hydraulic composite wet joint designed to address the defects of friction damage and poor contact in current wet joint direct contact power transmission. The joint uses claw docking and wireless energy transmission to achieve the composite transmission of hydraulic and electric power. Firstly, we independently designed a DC power supply inverter circuit, rectification circuit, and wireless power transmission coil assembly to form a wireless power transmission system. We also conducted testing and analysis on the wireless power transmission efficiency, which exceeded 60%. When the input voltage was above 80 V, the output power was greater than 60 W, meeting the design requirements. Secondly, the mechanical structure of the new electro-hydraulic signal wet joint tool was optimized and its strength was verified. The simulation results showed that the maximum stress was 891.8 MPa, and the maximum deformation of the wet joint docking overall structure was 0.123 mm. The strength and deformation met the design requirements. The hydraulic and electrical connectivity indoor tests were conducted on the electromagnetic coupling wet joint, and all aspects of transmission were normal, thus forming a design scheme for the underground electromagnetic coupling electro-hydraulic signal wet joint. The wireless transmission type electro-hydraulic signal wet joint designed in this article is of great significance for accelerating the promotion and application process of deepwater intelligent completion systems.
Two Competing Religious Traditions Underlying the Façade-Type Architectural Models from Ancient Western Asia
Authors: Sugimoto David T.
Published: February 2025

This study clarifies the religious traditions underlying the façade-type clay architectural models unearthed from ancient Western Asia by analyzing their iconography apropos each period. The façade-type models considered in this study are one-storied, with a distinct façade, one large opening, and the cella crafted in the niche, three-dimensional, or jar style. The analysis reveals (a) the distribution areas of the most common niche style shifted from the third millennium BC Mesopotamia, through the Middle and Late Bronze Ages and the early Iron Age Levant area, to the Iron Age IIB–C Phoenician areas; (b) the jar style is known only from the second millennium BC Levant and eventually merged with the niche style; and (c) the three-dimensional style is found sporadically. The earlier examples have either a male or a female figure inside the gate, although some have neither. The Levantine examples are without figural representations or with a bird, lions, and female figurines. It may be concluded that two competing religious traditions continued for three millennia, wherein the models with a male figure or without figures represent the tradition of the sovereign god originated in Sumer, whereas those with female figures and decorative motifs reflect the Inana-Ištar cult.
A Satellite Full-Waveform Laser Decomposition Method for Forested Areas Based on Hidden Peak Detection and Adaptive Genetic Optimization
Authors: Zhang Fangxv , Wang Xiao , Wang Leiguang , Mo Fan , Zhao Liping , Yang Xiaomeng , Lv Xin , Xie Junfeng
Published: February 2025

Laser waveform data that contain rich three-dimensional structural object information hold significant value in forest resource monitoring. However, traditional waveform decomposition algorithms are often constrained by complex waveform structures and depend on the initial parameter selections, which affect the accuracy and robustness of the results. To address the issues of the strong dependence on initial parameters, susceptibility to local optima, and difficulty in detecting hidden peaks during waveform overlap in the traditional satellite laser waveform decomposition algorithms, this study proposes a waveform decomposition method that combines hidden peak detection and an adaptive genetic algorithm (HAGA). This method uses hidden peak detection algorithms to improve the accurate extraction of the Gaussian components from the original waveform and provides the initial parameters. The high-precision extraction of waveform parameters is achieved through the adaptive genetic algorithm (AGA) combined with Levenberg–Marquardt (LM) optimization. In the experimental validation, the proposed method outperformed the traditional methods in both waveform decomposition fitting accuracy and tree height extraction. The average waveform decomposition accuracy Rmean2 for more than 2000 laser spots reaches 0.955, whereas the RMSE of the tree height extractions is better than 2 m, demonstrating strong robustness and applicability.