bluuebunny/crossref_metadata_2025 huggingface dataset:
Tendency to Worry and Fear of Mental Health during Italy’s COVID-19 Lockdown
Authors: Baiano Chiara , Zappullo Isa ,   the LabNPEE Group, Conson Massimiliano
Published: August 2020

Background: We tested whether the tendency to worry could affect psychological responses to quarantine by capitalizing on the opportunity of having collected data before the COVID-19 outbreak on measures of worry, anxiety, and trait mindfulness in a group of university students. Methods: Twenty-five participants completed self-report measures assessing worry (Penn State Worry Questionnaire, PSWQ), anxiety (Anxiety Sensitivity Index, ASI-3), and trait mindfulness (Mindful Attention Awareness Scale, MAAS) at T0 (pre-lockdown, 4 November 2019–17 February 2020) and T1 (at the end of lockdown, 26 April–30 April 2020). We compared assessments at the two time points in the whole sample and in high and low worriers (defined at T0 by scores on PSWQ respectively above and below 1.5 SD from mean of the Italian normative sample). Outcomes: High worriers showed at T1 a significant increase of anxiety sensitivity and fear of mental health in comparison to low worriers. Moreover, in the whole sample, at T1 trait mindfulness was inversely related to worry and fear of mental health. Interpretation: A valuable approach to support individuals experiencing anxiety related to the COVID-19 outbreak could be represented by mindfulness-based interventions improving the ability to focus attention and awareness on the present moment.
Objective Measures to Assess Active Commuting Physical Activity to School in Young People: A Systematic Review Protocol and Practical Considerations
Authors: Campos-Garzón Pablo , Sevil-Serrano Javier , Barranco-Ruíz Yaira , Chillón Palma
Published: August 2020

There are no systematic reviews that have identified the existing studies assessing active commuting physical activity (PA) to and from (to/from) school using objective measures, as well as the contribution of both walking and cycling to/from school to PA levels. To fill this gap in the literature, this systematic review will aim (a) to identify existing studies that assess active commuting PA to/from school with objective measures in young people and to examine the contribution of walking and cycling to/from school to PA levels, and (b) to propose an appropriate methodology and practical considerations to assess active commuting PA to/from school based on the studies identified. The review protocol was registered in PROSPERO (CRD42020162004). We will conduct a systematic search up to 2020 in five databases: PubMed, Web of Science, SPORTdiscuss, Cochrane Library, and National Transportation Library. Both the risk of bias and the quality of the identified studies will be evaluated through different instruments according to the design of each study. This systematic review will help to choose the most appropriate objective measures to assess active commuting PA to/from school and to promote walking and cycling to/from school to increase PA levels.
Diet Quality among Women with Previous Gestational Diabetes Mellitus in Rural Areas of Hunan Province
Authors: Li Mingshu , Shi Jingcheng , Luo Jing , Long Qing , Yang Qiping , OuYang Yufeng , Liu Hanmei , Lin Qian , Guo Jia
Published: August 2020

Diet quality is critical for postpartum type 2 diabetes mellitus (T2DM) progression among women with a history of gestational diabetes mellitus (GDM). The Chinese Healthy Eating Index (CHEI) is a dietary index developed according to the latest Chinese Dietary Guidelines (CDG-2016). Our study aimed to assess the diet quality of women with previous GDM in rural areas of Hunan Province by applying the CHEI. Women with previous GDM in two counties of Hunan were enrolled. Their food intake data, which were used to calculate their CHEI scores, were collected by a 24-h dietary recall. The association of CHEI with sociodemographic and anthropometric variables was evaluated via linear regression models. 404 women were included in the final analysis. The mean score of the total CHEI was 54.9 (SD 7.9). The components of whole grains and mixed beans, seeds and nuts, tubers, dairy, and poultry scored extremely low. Ethnic minority groups and women younger than 30 years had lower CHEI scores. Our study observed an unsatisfactory diet quality among women with previous GDM in rural areas of Hunan Province. Future dietary education focusing on the CDG is needed to improve their diet quality and help in T2DM prevention among this population, especially young and ethnic minority women.
Development of a Novel Framework to Propose New Strategies for Automated External Defibrillators Deployment Targeting Residential Out-Of-Hospital Cardiac Arrests: Application to the City of Milan
Authors: Lorenzo Gianquintieri , Maria Antonia Brovelli , Piero Maria Brambilla , Andrea Pagliosa , Guido Francesco Villa , Enrico Gianluca Caiani
Published: August 2020

Public Access Defibrillation (PAD) is the leading strategy in reducing time to first defibrillation in cases of Out-Of-Hospital Cardiac Arrest (OHCA), but PAD programs are underperforming considering their potentiality. Our aim was to develop an analysis and optimization framework, exploiting georeferenced information processed with Geographic Information Systems (GISs), specifically targeting residential OHCAs. The framework, based on an historical database of OHCAs, location of Automated External Defibrillators (AEDs), topographic and demographic information, proposes new strategies for AED deployment focusing on residential OHCAs, where performance assessment was evaluated using AEDs “catchment area” (area that can be reached within 6 min walk along streets). The proposed framework was applied to the city of Milan, Lombardy (Italy), considering the OHCA database of four years (2015–2018), including 8152 OHCA, of which 7179 (88.06%) occurred in residential locations. The proposed strategy for AEDs deployment resulted more effective compared to the existing distribution, with a significant improvement (from 41.77% to 73.33%) in OHCAs’ spatial coverage. Further improvements were simulated with different cost scenarios, resulting in more cost-efficient solutions. Results suggest that PAD programs, either in brand-new territories or in further improvements, could significantly benefit from a comprehensive planning, based on mathematical models for risk mapping and on geographical tools.
NanoBiT System and Hydrofurimazine for Optimized Detection of Viral Infection in Mice—A Novel in Vivo Imaging Platform
Authors: Gaspar Natasa , Zambito Giorgia , Dautzenberg Iris J. C. , Cramer Steve J. , Hoeben Rob C. , Lowik Clemens , Walker Joel R. , Kirkland Thomas A. , Smith Thomas P. , van Weerden Wytske M. , de Vrij Jeroen , Mezzanotte Laura
Published: August 2020

Reporter genes are used to visualize intracellular biological phenomena, including viral infection. Here we demonstrate bioluminescent imaging of viral infection using the NanoBiT system in combination with intraperitoneal injection of a furimazine analogue, hydrofurimazine. This recently developed substrate has enhanced aqueous solubility allowing delivery of higher doses for in vivo imaging. The small high-affinity peptide tag (HiBiT), which is only 11 amino-acids in length, was engineered into a clinically used oncolytic adenovirus, and the complementary large protein (LgBiT) was constitutively expressed in tumor cells. Infection of the LgBiT expressing cells with the HiBiT oncolytic virus will reconstitute NanoLuc in the cytosol of the cell, providing strong bioluminescence upon treatment with substrate. This new bioluminescent system served as an early stage quantitative viral transduction reporter in vitro and also in vivo in mice, for longitudinal monitoring of oncolytic viral persistence in infected tumor cells. This platform provides novel opportunities for studying the biology of viruses in animal models.
Current Understanding of the Role of Cytoskeletal Cross-Linkers in the Onset and Development of Cardiomyopathies
Authors: Pecorari Ilaria , Mestroni Luisa , Sbaizero Orfeo
Published: August 2020

Cardiomyopathies affect individuals worldwide, without regard to age, sex and ethnicity and are associated with significant morbidity and mortality. Inherited cardiomyopathies account for a relevant part of these conditions. Although progresses have been made over the years, early diagnosis and curative therapies are still challenging. Understanding the events occurring in normal and diseased cardiac cells is crucial, as they are important determinants of overall heart function. Besides chemical and molecular events, there are also structural and mechanical phenomena that require to be investigated. Cell structure and mechanics largely depend from the cytoskeleton, which is composed by filamentous proteins that can be cross-linked via accessory proteins. Alpha-actinin 2 (ACTN2), filamin C (FLNC) and dystrophin are three major actin cross-linkers that extensively contribute to the regulation of cell structure and mechanics. Hereby, we review the current understanding of the roles played by ACTN2, FLNC and dystrophin in the onset and progress of inherited cardiomyopathies. With our work, we aim to set the stage for new approaches to study the cardiomyopathies, which might reveal new therapeutic targets and broaden the panel of genes to be screened.
Oxidative Photocyclization of Aromatic Schiff Bases in Synthesis of Phenanthridines and Other Aza-PAHs
Authors: Kos Martin , Žádný Jaroslav , Storch Jan , Církva Vladimír , Cuřínová Petra , Sýkora Jan , Císařová Ivana , Kuriakose Febin , Alabugin Igor. V.
Published: August 2020

The oxidative photocyclization of aromatic Schiff bases was investigated as a potential method for synthesis of phenanthridine derivatives, biologically active compounds with medical applications. Although it is possible to prepare the desired phenanthridines using such an approach, the reaction has to be performed in the presence of acid and TEMPO to increase reaction rate and yield. The reaction kinetics was studied on a series of substituted imines covering the range from electron-withdrawing to electron-donating substituents. It was found that imines with electron-withdrawing substituents react one order of magnitude faster than imines bearing electron-donating groups. The 1H NMR monitoring of the reaction course showed that a significant part of the Z isomer in the reaction is transformed into E isomer which is more prone to photocyclization. The portion of the Z isomer transformed showed a linear correlation to the Hammett substituent constants. The reaction scope was expanded towards synthesis of larger aromatic systems, namely to the synthesis of strained aromatic systems, e.g., helicenes. In this respect, it was found that the scope of oxidative photocyclization of aromatic imines is limited to the formation of no more than five ortho-fused aromatic rings.
N-(2′-Hydroxyphenyl)-2-Propylpentanamide (HO-AAVPA) Inhibits HDAC1 and Increases the Translocation of HMGB1 Levels in Human Cervical Cancer Cells
Authors: Sixto-López Yudibeth , Rosales-Hernández Martha Cecilia , Contis-Montes de Oca Arturo , Fragoso-Morales Leticia Guadalupe , Mendieta-Wejebe Jessica Elena , Correa-Basurto Ana María , Abarca-Rojano Edgar , Correa-Basurto José
Published: August 2020

N-(2′-hydroxyphenyl)-2-propylpentanamide (HO-AAVPA) is a VPA derivative designed to be a histone deacetylase (HDAC) inhibitor. HO-AAVPA has better antiproliferative effect than VPA in cancer cell lines. Therefore, in this work, the inhibitory effect of HO-AAVPA on HDAC1, HDAC6, and HDAC8 was determined by in silico and in vitro enzymatic assay. Furthermore, its antiproliferative effect on the cervical cancer cell line (SiHa) and the translocation of HMGB1 and ROS production were evaluated. The results showed that HO-AAVPA inhibits HDAC1, which could be related with HMGB1 translocation from the nucleus to the cytoplasm due to HDAC1 being involved in the deacetylation of HMGB1. Furthermore, an increase in ROS production was observed after the treatment with HO-AAVPA, which also could contribute to HMGB1 translocation. Therefore, the results suggest that one of the possible antiproliferative mechanisms of HO-AAVPA is by HDAC1 inhibition which entails HMGB1 translocation and ROS increased levels that could trigger the cell apoptosis.
Mitochondrial Genetic Drift after Nuclear Transfer in Oocytes
Authors: Yamada Mitsutoshi , Akashi Kazuhiro , Ooka Reina , Miyado Kenji , Akutsu Hidenori
Published: August 2020

Mitochondria are energy-producing intracellular organelles containing their own genetic material in the form of mitochondrial DNA (mtDNA), which codes for proteins and RNAs essential for mitochondrial function. Some mtDNA mutations can cause mitochondria-related diseases. Mitochondrial diseases are a heterogeneous group of inherited disorders with no cure, in which mutated mtDNA is passed from mothers to offspring via maternal egg cytoplasm. Mitochondrial replacement (MR) is a genome transfer technology in which mtDNA carrying disease-related mutations is replaced by presumably disease-free mtDNA. This therapy aims at preventing the transmission of known disease-causing mitochondria to the next generation. Here, a proof of concept for the specific removal or editing of mtDNA disease-related mutations by genome editing is introduced. Although the amount of mtDNA carryover introduced into human oocytes during nuclear transfer is low, the safety of mtDNA heteroplasmy remains a concern. This is particularly true regarding donor-recipient mtDNA mismatch (mtDNA–mtDNA), mtDNA-nuclear DNA (nDNA) mismatch caused by mixing recipient nDNA with donor mtDNA, and mtDNA replicative segregation. These conditions can lead to mtDNA genetic drift and reversion to the original genotype. In this review, we address the current state of knowledge regarding nuclear transplantation for preventing the inheritance of mitochondrial diseases.
Heterologous Hydrogenase Overproduction Systems for Biotechnology—An Overview
Authors: Fan Qin , Neubauer Peter , Lenz Oliver , Gimpel Matthias
Published: August 2020

Hydrogenases are complex metalloenzymes, showing tremendous potential as H2-converting redox catalysts for application in light-driven H2 production, enzymatic fuel cells and H2-driven cofactor regeneration. They catalyze the reversible oxidation of hydrogen into protons and electrons. The apo-enzymes are not active unless they are modified by a complicated post-translational maturation process that is responsible for the assembly and incorporation of the complex metal center. The catalytic center is usually easily inactivated by oxidation, and the separation and purification of the active protein is challenging. The understanding of the catalytic mechanisms progresses slowly, since the purification of the enzymes from their native hosts is often difficult, and in some case impossible. Over the past decades, only a limited number of studies report the homologous or heterologous production of high yields of hydrogenase. In this review, we emphasize recent discoveries that have greatly improved our understanding of microbial hydrogenases. We compare various heterologous hydrogenase production systems as well as in vitro hydrogenase maturation systems and discuss their perspectives for enhanced biohydrogen production. Additionally, activities of hydrogenases isolated from either recombinant organisms or in vivo/in vitro maturation approaches were systematically compared, and future perspectives for this research area are discussed.
Regulation of Female Folliculogenesis by Tsp1a in Nile Tilapia (Oreochromis niloticus)
Authors: Jie Mimi , Ma He , Zhou Li , Wu Jiahong , Li Minghui , Liu Xingyong , Wang Deshou
Published: August 2020

TSP1 was reported to be involved in multiple biological processes including the activation of TGF-β signaling pathways and the regulation of angiogenesis during wound repair and tumor growth, while its role in ovarian folliculogenesis remains to be elucidated. In the present study, Tsp1a was found to be expressed in the oogonia and granulosa cells of phase I to phase IV follicles in the ovaries of Nile tilapia by immunofluorescence. tsp1a homozygous mutants were generated by CRISPR/Cas9. Mutation of tsp1a resulted in increased oogonia, reduced secondary growth follicles and delayed ovary development. Expression of the cell proliferation marker PCNA was significantly up-regulated in the oogonia of the mutant ovaries. Furthermore, transcriptomic analysis revealed that expressions of DNA replication related genes were significantly up-regulated, while cAMP and MAPK signaling pathway genes which inhibit cell proliferation and promote cell differentiation were significantly down-regulated. In addition, aromatase (Cyp19a1a) expression and serum 17β-estradiol (E2) concentration were significantly decreased in the mutants. These results indicated that lacking tsp1a resulted in increased proliferation and inhibited differentiation of oogonia, which in turn, resulted in increased oogonia, reduced secondary growth follicles and decreased E2. Taken together, our results indicated that tsp1a was essential for ovarian folliculogenesis in Nile tilapia.
Therapeutic Drug Monitoring Is a Feasible Tool to Personalize Drug Administration in Neonates Using New Techniques: An Overview on the Pharmacokinetics and Pharmacodynamics in Neonatal Age
Authors: De Rose Domenico Umberto , Cairoli Sara , Dionisi Marco , Santisi Alessandra , Massenzi Luca , Goffredo Bianca Maria , Dionisi-Vici Carlo , Dotta Andrea , Auriti Cinzia
Published: August 2020

Therapeutic drug monitoring (TDM) should be adopted in all neonatal intensive care units (NICUs), where the most preterm and fragile babies are hospitalized and treated with many drugs, considering that organs and metabolic pathways undergo deep and progressive maturation processes after birth. Different developmental changes are involved in interindividual variability in response to drugs. A crucial point of TDM is the choice of the bioanalytical method and of the sample to use. TDM in neonates is primarily used for antibiotics, antifungals, and antiepileptic drugs in clinical practice. TDM appears to be particularly promising in specific populations: neonates who undergo therapeutic hypothermia or extracorporeal life support, preterm infants, infants who need a tailored dose of anticancer drugs. This review provides an overview of the latest advances in this field, showing options for a personalized therapy in newborns and infants.
Hexobarbital Sleep Test for Predicting the Susceptibility or Resistance to Experimental Posttraumatic Stress Disorder
Authors: Komelkova Maria , Manukhina Eugenia , Downey H. Fred , Sarapultsev Alexey , Cherkasova Olga , Kotomtsev Viacheslav , Platkovskiy Pavel , Fedorov Stanislav , Sarapultsev Petr , Tseilikman Olga , Tseilikman David , Tseilikman Vadim
Published: August 2020

Hexobarbital sleep test (HST) was performed in male Wistar rats (hexobarbital 60 mg/kg, i.p.) 30 days prior to stress exposure. Based on the duration of hexobarbital-induced sleep, rats were divided into two groups, animals with high intensity (fast metabolizers (FM), sleep duration <15 min) or low intensity of hexobarbital metabolism (slow metabolizers (SM), sleep duration ≥15 min). The SM and FM groups were then divided into two subgroups: unstressed and stressed groups. The stressed subgroups were exposed to predator scent stress for 10 days followed by 15 days of rest. SM and FM rats from the unstressed group exhibited different behavioral and endocrinological patterns. SM showed greater anxiety and higher corticosterone levels. In stressed animals, anxiety-like posttraumatic stress disorder (PTSD) behavior was aggravated only in SM. Corticosterone levels in the stressed FM, PTSD-resistant rats, were lower than in unstressed SM. Thus, HST was able to predict the susceptibility or resistance to experimental PTSD, which was consistent with the changes in glucocorticoid metabolism.
Challenges Faced by Maltese Students Studying Advanced Level Physics
Authors: Pullicino Nathan , Bonello Charles
Published: August 2020

One of the aims of the Secondary Education Certificate (SEC) Physics syllabus is “to provide the basis for further study of the subject”. This research determined the extent to which the syllabus is fulfilling this aim. In this study, seven post-secondary Physics teachers participated in semi-structured interviews and 200 students provided feedback to a questionnaire. Areas in which the SEC Physics syllabus is not preparing students well enough to further their studies in the subject were identified and suggestions were given to help improve the situation. This study confirmed that there is an academic disparity between SEC and Advanced Matriculation (AM) Physics. This disparity is highlighted in the problem-solving skills necessary for success at both levels, mathematical physics, language and in concepts which are highly abstract. The study also confirmed that there is a large amount of rote learning involved in SEC level Physics. As a result, students learn superficially and struggle to grasp the complex concepts taught in A-level Physics. In order to prepare students better for post-secondary education, SEC Physics students should be asked to answer questions which involve higher levels of thinking and to solve more complex mathematical problems. Furthermore, more frequent practical sessions, a greater degree of student involvement and a greater emphasis on the link between theoretical ideas and practical work is also recommended. A shift of emphasis is required from teaching content to teaching higher order thinking skills.
Impacts of Dietary Nutritional Composition on Larval Development and Adult Body Composition in the Yellow Fever Mosquito (Aedes aegypti)
Authors: van Schoor Tess , Kelly Erin Taylor , Tam Nicole , Attardo Geoffrey Michael
Published: August 2020

Background: the mosquito Aedes aegypti (Ae. aegypti) is an important vector of arboviruses, including Zika, Dengue, and Chikungunya. The dietary requirements of larval Ae. aegypti are not well understood and likely impact developmental and physiological parameters knowledge of which could be important for vector control. This study examines the effects nutrition has on growth and development of larval Ae. aegypti of laboratory-reared Rockefeller strain mosquitoes. Methods: mosquito larvae were split into five feeding groups with diets providing different ratios of protein and carbohydrates. Each group received autolyzed Brewer’s yeast (AY - high-protein), and/or rice flour (RF—high-carbohydrate). The groups were monitored to record larval developmental times, adult sizes and nutritional stores. Results: the 100% AY group failed to pupate, suggesting the AY alone is either lacking in critical nutrients or is toxic at higher concentrations. The 100% RF group resulted in the smallest adults that took the longest time to reach pupation. Of the remaining groups, the 25% AY/75% RF (Med–low) diet yielded adult mosquitoes with highest average weight, wing length, and lipid stores relative to the other diets. Conclusions: the dietary requirements for development, body size, and nutrient stores of Ae. aegypti mosquitoes appear to be dependent on a relatively low but essential proportion of dietary protein to carbohydrates to achieve optimal developmental outcomes.
Dynamic Expression Profiles of β-Catenin during Murine Cardiac Valve Development
Authors: Guo Lilong , Glover Janiece , Risner Alyssa , Wang Christina , Fulmer Diana , Moore Kelsey , Gensemer Cortney , Rumph Mary Kate , Moore Reece , Beck Tyler , Norris Russell A.
Published: August 2020

β-catenin has been widely studied in many animal and organ systems across evolution, and gain or loss of function has been linked to a number of human diseases. Yet fundamental knowledge regarding its protein expression and localization remains poorly described. Thus, we sought to define whether there was a temporal and cell-specific regulation of β-catenin activities that correlate with distinct cardiac morphological events. Our findings indicate that activated nuclear β-catenin is primarily evident early in gestation. As development proceeds, nuclear β-catenin is down-regulated and becomes restricted to the membrane in a subset of cardiac progenitor cells. After birth, little β-catenin is detected in the heart. The co-expression of β-catenin with its main transcriptional co-factor, Lef1, revealed that Lef1 and β-catenin expression domains do not extensively overlap in the cardiac valves. These data indicate mutually exclusive roles for Lef1 and β-catenin in most cardiac cell types during development. Additionally, these data indicate diverse functions for β-catenin within the nucleus and membrane depending on cell type and gestational timing. Cardiovascular studies should take into careful consideration both nuclear and membrane β-catenin functions and their potential contributions to cardiac development and disease.
Comparison of Drusen Volume Assessed by Two Different OCT Devices
Authors: Beck Marco , Joshi Devika S. , Berger Lieselotte , Klose Gerd , De Zanet Sandro , Mosinska Agata , Apostolopoulos Stefanos , Ebneter Andreas , Zinkernagel Martin S. , Wolf Sebastian , Munk Marion R.
Published: August 2020

To compare drusen volume between Heidelberg Spectral Domain (SD-) and Zeiss Swept-Source (SS) PlexElite Optical Coherence Tomography (OCT) determined by manual and automated segmentation methods. Thirty-two eyes of 24 patients with Age-Related Macular Degeneration (AMD) and drusen maculopathy were included. In the central 1 and 3 mm ETDRS circle drusen volumes were calculated and compared. Drusen segmentation was performed using automated manufacturer algorithms of the two OCT devices. Then, the automated segmentation was manually corrected and compared and finally analyzed using customized software. Though on SD-OCT, there was a significant difference of mean drusen volume prior to and after manual correction (mean difference: 0.0188 ± 0.0269 mm3, p < 0.001, corr. p < 0.001, correlation of r = 0.90), there was no difference found on SS-OCT (mean difference: 0.0001 ± 0.0003 mm3, p = 0.262, corr. p = 0.524, r = 1.0). Heidelberg-acquired mean drusen volume after manual correction was significantly different from Zeiss-acquired drusen volume after manual correction (mean difference: 0.1231 ± 0.0371 mm3, p < 0.001, corr. p < 0.001, r = 0.68). Using customized software, the difference of measurements between both devices decreased and correlation among the measurements improved (mean difference: 0.0547 ± 0.0744 mm3, p = 0.02, corr. p = 0.08, r = 0.937). Heidelberg SD-OCT, the Zeiss PlexElite SS-OCT, and customized software all measured significantly different drusen volumes. Therefore, devices/algorithms may not be interchangeable. Third-party customized software helps to minimize differences, which may allow a pooling of data of different devices, e.g., in multicenter trials.
The “No ARSA” Sign: A Novel Method of Prenatal Screening for Aberrant Right Subclavian Artery
Authors: Kassif Eran , Tsur Abraham , Shust-Barequet Shir , Raviv Oshrat , Kushnir Anya , Abu Snenh Samar , Achiron Reuven , Mazaki-Tovi Shali , Weisz Boaz , Salem Yishay , Weissbach Tal
Published: August 2020

An aberrant right subclavian artery (ARSA) can be overlooked by the conventional method as described by Chaoui et al., due to acoustic shadowing. The aim of this study was to evaluate the feasibility and accuracy of a novel screening method for ARSA by demonstrating the brachiocephalic artery bifurcation, referred to as the “No ARSA” sign. A prospective study conducted at a tertiary care center between 2018 and 2019 included unselected pregnant patients at a median gestational age of 15.1 (14.2–22.1; IQR (inter-quartile range)) weeks, who had been referred for a routine or targeted anomaly scan. All participants were scanned for the presence or absence of ARSA using both the conventional and the novel “No ARSA” methods for validation purposes. A total of 226 unselected patients were enrolled in the study. The “No ARSA” sign was visualized in 218 fetuses (96.5%). In the remaining 8 cases (3.5%), the “No ARSA” sign was not demonstrated. In these fetuses, an ARSA was visualized by the conventional method. The new method exhibited 100% feasibility and was in complete agreement with the conventional method. Intra- and inter-observer agreement was excellent (κ = 1). The results of the study suggest that the “No ARSA” sign is an efficient and reliable screening tool for ARSA.
Frailty and Sarcopenia Assessment upon Hospital
Admission to Internal Medicine Predicts Length of
Hospital Stay and Re-Admission: A Prospective
Study of 980 Patients
Authors: Anani Sapir , Goldhaber Gal , Brom Adi , Lasman Nir , Turpashvili Natia , Shenhav-saltzman Gilat , Avaky Chen , Negru Liat , Agbaria Muhamad , Ariam Sigalit , Portal Doron , Wasserstrum Yishay , Segal Gad
Published: August 2020

Background: Frailty and sarcopenia are associated with frequent hospitalizations and poor clinical outcomes in geriatric patients. Ascertaining this association for younger patients hospitalized in internal medicine departments could help better prognosticate patients in the realm of internal medicine. Methods: During a 1-year prospective study in an internal medicine department, we evaluated patients upon admission for sarcopenia and frailty. We used the FRAIL questionnaire, blood alanine-amino transferase (ALT) activity, and mid-arm muscle circumference (MAMC) measurements. Results: We recruited 980 consecutive patients upon hospital admission (median age 72 years (IQR 65–79); 56.8% males). According to the FRAIL questionnaire, 106 (10.8%) patients were robust, 368 (37.5%) pre-frail, and 506 (51.7%) were frail. The median ALT value was 19IU/L (IQR 14–28). The median MAMC value was 27.8 (IQR 25.7–30.2). Patients with low ALT activity level (<17IU/L) were frailer according to their FRAIL score (3 (IQR 2–4) vs. 2 (IQR 1–3); p < 0.001). Higher MAMC values were associated with higher ALT activity, both representing robustness. The rate of 30 days readmission in the whole cohort was 17.4%. Frail patients, according to the FRAIL score (FS), had a higher risk for 30 days readmission (for FS > 2, HR = 1.99; 95CI = 1.29–3.08; p = 0.002). Frail patients, according to low ALT activity, also had a significantly higher risk for 30 days readmission (HR = 2.22; 95CI = 1.26–3.91; p = 0.006). After excluding patients whose length of stay (LOS) was ≥10 days, 252 (27.5%) stayed in-hospital for 4 days or longer. Frail patients according to FS had a higher risk for LOS ≥4 days (for FS > 2, HR = 1.87; 95CI = 1.39–2.52; p < 0.001). Frail patients, according to low ALT activity, were also at higher risk for LOS ≥4 days (HR = 1.87; 95CI = 1.39–2.52; p < 0.001). MAMC values were not correlated with patients’ LOS or risk for re-admission. Conclusion: Frailty and sarcopenia upon admission to internal medicine departments are associated with longer hospitalization and increased risk for re-admission.
Torsional Vibration Stress and Fatigue Strength Analysis of Marine Propulsion Shafting System Based on Engine Operation Patterns
Authors: Song Myeong-Ho , Pham Xuan Duong , Vuong Quang Dao
Published: August 2020

Modern merchant ships use marine propulsion systems equipped with an ultra-long-stroke diesel engine that directly drives a large slow-turning propeller. Such systems use fewer cylinders and generate greater power at slower shaft speeds, which affords improved propulsion performance as well as low repair and maintenance costs. However, this also results in higher torsional vibrations, which can lead to the fatigue of the shafting system. Tests performed on various marine propulsion systems with 5- to 7-cylinder engines have shown that engines with fewer cylinders exhibit a correspondingly wider barred speed range (BSR) and higher torsional vibration stresses. Thus, it is necessary to investigate the optimal engine operation patterns required to quickly pass the BSR with smaller torsional vibration. In this study, we carried out a series of BSR passage experiments during actual sea trials to evaluate the intermediate shaft performance under different engine operation patterns. The fractional damage accumulations due to transient torsional vibration stresses were calculated to estimate the fatigue lifetime of the shafting system. Our analysis results show that the torsional fatigue damage during BSR decelerations are small and negligible; however, the fractional damage during accelerations is a matter of concern. Our study determines the optimal main engine operation pattern for quick passage through the BSR with the smallest torsional vibration amplitudes and the least fractional damage accumulation, which can therefore extend the fatigue lifetime of the entire propulsion shafting system. Based on this analysis, we also suggest the optimum engine pattern for safe BSR passage.
Seasonal Predictions of Shoreline Change, Informed by Climate Indices
Authors: Hilton Dan , Davidson Mark , Scott Tim
Published: August 2020

With sea level rise accelerating and coastal populations increasing, the requirement of coastal managers and scientists to produce accurate predictions of shoreline change is becoming ever more urgent. Waves are the primary driver of coastal evolution, and much of the interannual variability of the wave conditions in the Northeast Atlantic can be explained by broadscale patterns in atmospheric circulation. Two of the dominant climate indices that capture the wave climate in western Europe’s coastal regions are the ‘Western Europe Pressure Anomaly’ (WEPA) and ‘North Atlantic Oscillation’ (NAO). This study utilises a shoreline prediction model (ShoreFor) which is forced by synthetic waves to investigate whether forecasts can be improved when the synthetic wave generation algorithm is informed by relevant climate indices. The climate index-informed predictions were tested against a baseline case where no climate indices were considered over eight winter periods at Perranporth, UK. A simple adaption to the synthetic wave-generating process has allowed for monthly climate index values to be considered before producing the 103 random waves used to force the model. The results show that improved seasonal predictions of shoreline change are possible if climate indices are known a priori. For NAO, modest gains were made over the uninformed ShoreFor model, with a reduction in average root mean square error (RMSE) of 7% but an unchanged skill score. For WEPA, the gains were more significant, with the average RMSE 12% lower and skill score 5% higher. Highlighted is the importance of selecting an appropriate index for the site location. This work suggests that better forecasts of shoreline change could be gained from consideration of a priori knowledge of climatic indices in the generation of synthetic waves.
The Impact of Tides on the Bay of Biscay Dynamics
Authors: Karagiorgos John , Vervatis Vassilios , Sofianos Sarantis
Published: August 2020

The impact of tides on the Bay of Biscay dynamics is investigated by means of an ocean model twin-experiment, consisted of two simulations with and without tidal forcing. The study is based on a high-resolution (1/36∘) regional configuration of NEMO (Nucleus for European Modelling of the Ocean) performing one-year simulations. The results highlight the imprint of tides on the thermohaline properties and circulation patterns in three distinct dynamical areas in the model domain: the abyssal plain, the Armorican shelf and the English Channel. When tides are activated, the bottom stress is increased in the shelf areas by about two orders of magnitude with respect to the open ocean, subsequently enhancing vertical mixing and weakening stratification in the bottom boundary layer. The most prominent feature reproduced only when tides are modelled, is the Ushant front near the entrance of the English Channel. Tides appear also to constrain the freshwater transport of rivers from the continental shelf to the open ocean. The spectral analysis revealed that the tidal forcing contributes to the SSH variance at high frequencies near the semidiurnal band and to the open ocean mesoscale and small-scale features in the presence of summer stratification pattern.
Land-Use Dynamics in Transport-Impacted Urban Fabric: A Case Study of Martin–Vrútky, Slovakia
Authors: Nozdrovická Jana , Dostál Ivo , Petrovič František , Jakab Imrich , Havlíček Marek , Skokanová Hana , Falťan Vladimír , Mederly Peter
Published: August 2020

The paper evaluates landscape development, land-use changes, and transport infrastructure variations in the city of Martin and the town of Vrútky, Slovakia, over the past 70 years. It focuses on analyses of the landscape structures characterizing the study area in several time periods (1949, 1970, 1993, 2003); the past conditions are then compared with the relevant current structure (2018). Special attention is paid to the evolution of the landscape elements forming the transport infrastructure. The development and progressive changes in traffic intensities are presented in view of the resulting impact on the formation of the landscape structure. The research data confirm the importance of transport as a force determining landscape changes, and they indicate that while railroad accessibility embodied a crucial factor up to the 1970s, the more recent decades were characterized by a gradual shift to road transport.
Optimizing Long-Lasting Insecticidal Nets Campaign in Ivory Coast
Authors: de Brito Irineu , Uneddu Silvia , Maspero Emma , Gonçalves Paulo
Published: August 2020

This research supports the United Nations Children’s Fund’s (UNICEF) conceptualization, planning and implementation of a campaign for distribution of more than 12 million mosquito nets in Ivory Coast. Procured from four different suppliers in Asia, the nets were transported to the two ports in Ivory Coast before being pre-positioned at 71 Health Districts across the country, a mixed integer network flow model identifies optimal transport options. The process of modeling and the model developed in this paper brought a significant understanding of the problem and, consequently, a reduction in the overall procurement and logistics costs. The implications of using mathematical modeling by practitioners as a tool which contributes to solve humanitarian logistics problems are significant. Mathematical models, like linear programming, can greatly support overall decision-making within humanitarian organizations by helping to ensure that limited resources are used in the most cost-effective and efficient manner. However, it is important to ensure consultations with and involvement by on the ground practitioners to ensure developed solutions assessed to fit the operating context before being implemented.
The Effect of Poly(Vinyl Chloride) Powder Addition on the Thermomechanical Properties of Epoxy Composites Reinforced with Basalt Fiber
Authors: Matykiewicz Danuta , Sałasińska Kamila , Barczewski Mateusz
Published: August 2020

The aim of the article was to determine the effect of the poly(vinyl chloride) additive (PVC) on the thermomechanical and fire properties of epoxy composites reinforced with basalt fabric. Ten-layered composites with 2.5, 5 and 10 wt.% of PVC powder were fabricated using hand lay-up. The following features were evaluated for composites: structure (by scanning electron microscopy, SEM), thermomechanical properties (by dynamical thermomechanical analysis, DMTA), mechanical properties (in bending, tensile and interlaminar shear strength tests), hardness (using the Barcol method), thermal stability (by thermogravimetry, TGA) and fire behavior (using a cone calorimeter). It was found that the introduction of micron PVC powder into the epoxy matrix improved the thermomechanical properties of composites, such as storage module, and mechanical properties, such as flexural strength and modulus, as well as hardness.
Spatial Distribution Evolution of Residual Stress and Microstructure in Laser-Peen-Formed Plates
Authors: Zhang Zheng , Huang Wen , Lu Guoxin , Zhang Yongkang
Published: August 2020

Residual stress in structural components is crucial as it affects both service performance and safety. To investigate the evolution of residual stress in a laser-peen-formed panel, this study adopted two plate samples of thickness 3 and 9 mm instead of the conventional Almen strip. The two plates were peened with an identical energy density of 10.99 GW/cm2. The residual stress across the entire section was determined using a slitting method, and near-surface stress was then verified by X-ray diffraction. Furthermore, cross-sectional variation in hardness and microstructure were characterized to understand the residual stress evolution. The experimental results showed that different thicknesses resulted in distinct spatial distributions of residual stress. The 3-mm plate demonstrated a shallow (0.5 mm) and lower compressive stress magnitude (−270 MPa) compared with a deeper (1 mm) and higher compressive stress (−490 MPa) in the 9-mm plate. Further analysis revealed that the deformation compatibility during the forming process inevitably leads to a stress compensation effect on the peened side. The decrease in the depth and magnitude of the compressive residual stress in the thin plate was mainly attributed to low stiffness and large deflection.
Cyclodextrin Complexation Improves the Solubility and Caco-2 Permeability of Chrysin
Authors: Fenyvesi Ferenc , Nguyen Thi Le Phuong , Haimhoffer Ádám , Rusznyák Ágnes , Vasvári Gábor , Bácskay Ildikó , Vecsernyés Miklós , Ignat Simona-Rebeca , Dinescu Sorina , Costache Marieta , Ciceu Alina , Hermenean Anca , Váradi Judit
Published: August 2020

Chrysin is a bioflavonoid that can be found in natural products such as honey and propolis, and it possesses several biological effects such as antioxidant, anti-inflammatory, and anti-cancer activity. However, it is poorly soluble in water, and its bioavailability is limited. The aim of this research is to investigate the chrysin solubilization capacity of different β-cylcodextrin derivatives and compare their biological activities. Chrysin was complexed with β-cyclodextrin (βCD), hydroxypropyl-β-, (HPBCD) sulfobutylether-β-, (SBECD), and randomly-methylated-β-cyclodextrin (RAMEB) by the lyophilization method in 1:1 and 1:2 molar ratios. The solubilities of the chrysin–cyclodextrin complexes were tested, and the solubilization abilities of cyclodextrins were studied by phase solubility experiments. The cytotoxicity of the complexes was measured by the MTT method, and the permeability enhancement was tested on Caco-2 monolayers. The solubility study showed that the complexes formed with RAMEB had the highest solubility in water. The phase solubility experiments confirmed the strongest interaction between RAMEB and chrysin. In the viability test, none of the complexes showed cytotoxicity up to 100 µM concentration. The permeability study revealed that both at 1:1 and 1:2 ratios, the RAMEB complexes were the most effective to enhance chrysin permeability through the Caco-2 monolayers. In conclusion, cyclodextrins, especially RAMEB, are suitable for improving chrysin solubility and absorption.
High-Temperature Deformation Behavior and Microstructural Characterization of Ti-35421 Titanium Alloy
Authors: Zhou Danying , Gao Hua , Guo Yanhua , Wang Ying , Dong Yuecheng , Dan Zhenhua , Chang Hui
Published: August 2020

A self-designed Ti-35421 (Ti-3Al-5Mo-4Cr-2Zr-1Fe wt%) titanium alloy is a new type of low-cost high strength titanium alloy. In order to understand the hot deformation behavior of Ti-35421 alloy, isothermal compression tests were carried out under a deformation temperature range of 750–930 °C with a strain rate range of 0.01–10 s−1 in this study. Electron backscatter diffraction (EBSD) was used to characterize the microstructure prior to and post hot deformation. The results show that the stress–strain curves have obvious yielding behavior at a high strain rate (>0.1 s−1). As the deformation temperature increases and the strain rate decreases, the α phase content gradually decreases in the α + β phase region. Meanwhile, spheroidization and precipitation of α phase are prone to occur in the α + β phase region. From the EBSD analysis, the volume fraction of recrystallized grains was very low, so dynamic recovery (DRV) is the dominant deformation mechanism of Ti-35421 alloy. In addition to DRV, Ti-35421 alloy is more likely to occur in continuous dynamic recrystallization (CDRX) than discontinuous dynamic recrystallization (DDRX).
Impact of Uncertainty in the Input Variables and Model Parameters on Predictions of a Long Short Term Memory (LSTM) Based Sales Forecasting Model
Authors: Goel Shakti , Bajpai Rahul
Published: August 2020

A Long Short Term Memory (LSTM) based sales model has been developed to forecast the global sales of hotel business of Travel Boutique Online Holidays (TBO Holidays). The LSTM model is a multivariate model; input to the model includes several independent variables in addition to a dependent variable, viz., sales from the previous step. One of the input variables, “number of active bookers per day”, is estimated for the same day as sales. This need for estimation requires the development of another LSTM model to predict the number of active bookers per day. The number of active bookers is variable, so the predicted is used as an input to the sales forecasting model. The use of a predicted variable as an input variable to another model increases the chance of uncertainty entering the system. This paper discusses the quantum of variability observed in sales predictions for various uncertainties or noise due to the estimation of the number of active bookers. For the purposes of this study, different noise distributions such as normalized, uniform, and logistic distributions are used, among others. Analyses of predictions demonstrate that the addition of uncertainty to the number of active bookers via dropouts as well as to the lagged sales variables leads to model predictions that are close to the observations. The least squared error between observations and predictions is higher for uncertainties modeled using other distributions (without dropouts) with the worst predictions being for Gumbel noise distribution. Gaussian noise added directly to the weights matrix yields the best results (minimum prediction errors). One possibility of this uncertainty could be that the global minimum of the least squared objective function with respect to the model weight matrix is not reached, and therefore, model parameters are not optimal. The two LSTM models used in series are also used to study the impact of corona virus on global sales. By introducing a new variable called the corona virus impact variable, the LSTM models can predict corona-affected sales within five percent (5%) of the actuals. The research discussed in the paper finds LSTM models to be effective tools that can be used in the travel industry as they are able to successfully model the trends in sales. These tools can be reliably used to simulate various hypothetical scenarios also.