bluuebunny/crossref_metadata_2025 huggingface dataset:
Validação de tecnologia educativa: suporte nutricional na cicatrização de feridas crônicas
Authors: Alfaia Lorenny Santos da Costa de , Pantoja Marla Cavalca , Nascimento Otiniel Moreira do , Pantoja Mauro de Sousa
Published: March 2022

Objetivo: elaborar e validar uma tecnologia educacional de suporte nutricional na cicatrização de feridas crônicas, como estratégia de apoio terapêutico para prática clínica dos profissionais de saúde. Metodologia: o desenvolvimento de pesquisa teve caráter prospectivo, transversal, com abordagem quantitativa analítica, por meio de validação de conteúdo através de uma escala tipo Likert pelo método de Pasquali, com a participação de 7 juízes avaliadores na área de saúde, com titulação mínima de especialistas; ao menos dois anos de experiência na área relacionada ao livro elaborado. Para avaliação de dados, adotou-se o índice de validade de conteúdo (IVC), sendo considerado válido o requisito com nível > 0,78. Resultados: o livro foi validado por uma equipe multiprofissional (nutricionistas, enfermeiros, médico e fisioterapeuta), com IVC global de 0,99 e percentual geral de aprovação de 94,2% classificados pelos especialistas. Considerações finais: os achados do estudo apontam que a tecnologia educativa sobre suporte nutricional na cicatrização de feridas crônicas foi validada pelos juízes especialistas participantes do processo de avaliação, permitindo uma grande ferramenta de aplicação na prática assistencial visando a recuperação do paciente.
POLA PENGELOLAAN WAKTU BELAJAR SAMBIL BEKERJA MAHASISWA FAKULTAS KEGURUAN DAN ILMU PENDIDIKAN UNIVERSITAS RIAU
Authors: Hafizah Nurul , Achmad Said Suhil , Fitrilinda Dafetta
Published: March 2022

Tujuan umum  penelitian ini untuk mendeskripsikan perilaku pengelolaan waktu belajar sambil bekerja pada mahasiswa FKIP Universitas Riau. Sedangkan tujuan khusus  adalah untuk mendeskripsikan profil mahasiswa belajar sambil bekerja. Penelitian ini dilakukan di Universitas Riau dengan subjek mahasiswa yang mengikuti perkuliahan pada Tahun akademis 2021-2022 sebanyak 3 orang, yang dipilih berdasarkan teknik purposive random dari 8 orang, karena memiliki prestasi belajar di atas rata-rata. Penelitian ini menggunakan pendekatan kualitatif deskriptif, yaitu memadukan pendekatan kualitatif dengan pendekatan kuantitatif. Pengumpulan dilakukan dengan teknik wawancara,observasi dan dokumentasi, dan keabsahan data dilakukan dengan triangulasi sumber dan teknik. Instrumen pengumpulan data adalah peneliti sendiri. Data dianalisis secara manual dimulai dari reduksi data, sajian data, penarikan kesimpulan. Kesimpulan penelitian menemukan bahwa pengelolaan waktu dilakukan dengan cara memprioritaskan belajar dari bekerja, hal ini dilakukan apabila ada waktu sudah mendesak, jika kedua sama-sama penting, maka dapat dikerjakan pada waktu yang sama, yaitu secara manual dan online; penyelesaian tugas dilakukan dengan cara mengerjakan lebih awal waktu dan mengantarkan tugas kepada dosen yang bersangkutan; memanfaatkan tempat dan waktu serta menghindari keramaian agar tidak membuang waktu untuk belajar; pengendalian waktu belajar dilakukan dengan cara membuat jadwal tertulis. Temuan lainnya terdapat tekanan dalam mengambil keputusan bila belajar sambil bekerj, serta alasan perekonomian keluarga.
HUBUNGAN KONSEP DIRI DENGAN KECERDASAN EMOSIONAL ANAK USIA 4-5 TAHUN DI TK SEHAT CERIA KECAMATAN MANDAU KABUPATEN BENGKALIS
Authors: Ratnasari Ratnasari , Novianti Ria , N Zulkifli
Published: March 2022

Membangun sebuah konsep diri sangatlah diperlukan oleh anak-anak. Kecerdasan emosional juga harus terbentuk untuk melahirkan control diri pada anak. Artikel ini membahas tentang hubungan antara konsep diri and kecerdasan emosional pada anak usia 4-5 tahun di TK Sehat Ceria Kecamatan Mandau Kabupaten Bengkalis Riau. Adapun sampel dalam penelitian ini adalah seluruh anak usia 4-5 tahun di TK Sehat Ceria Kecamatan Mandau Kabupaten Bengkalis berjumlah 30 orang anak. Metode yang digunakan survey dengan teknik korelasi. Teknik pengumpulan data yang digunakan adalah observasi data. Teknik analisis data adalah teknik statistik dengan menggunakan rumus pearson product moment. Hasil penelitian diketahui tingkat konsep diri anak berada dalam kategori baik dan tingkat kecerdasan emosional anak berada dalam kategori cukup. Hasil uji hipotesis menunjukkan nilai koefisien  korelasi sebesar rxy yaitu 0.6030 lebih besar dari rtabel  yaitu 0.364 (0.6030 > 0.364) dengan taraf signifikansi 0.000 < 0.05. Dengan demikian dapat disimpulkan bahwa terdapat hubungan antara konsep diri dengan kecerdasan emosional. Tingkat hubungan termasuk dalam kategori kuat  dengan nilai koefisien determinan sebesar 36.4%, artinya bahwa konsep diri memberi kontribusi sebesar 36.4% terhadap kecerdasan emosional, sisanya 64% ditentukan oleh variabel lain.
PENERAPAN MODEL QUANTUM TEACHING UNTUK MENINGKATKAN HASIL BELAJAR IPS SISWA KELAS V SD NEGERI 29 PEKANBARU
Authors: Fatimah Oza , Noviana Eddy , Guslinda Guslinda
Published: March 2022

Artikel ini menjelaskan tentang sebuah studi penelitian tindakan kelas. Artikel ini menunjukkan bagaimana siswa dapat meningkatkan hasil belajar mata pelajaran IPS melalui aplikasi model Quantum Teaching. Latar belakang penelitian dalam artikel ini dilihat dari rendahnya hasil belajar IPS siswa kelas V SD Negeri 29 Pekanbaru. Dari 37 orang siswa hanya 6 orang siswa (16.22%) yang mencapai KKM dan 31 orang siswa (83.78%) yang tidak mencapai KKM dengan nilai rata-rata kelas 55.35. Penelitian ini merupakan Penelitian Tindakan Kelas (PTK) Kolaboratif yang bertujuan untuk meningkatkan hasil belajar IPS melalui penerapan model Quantum Teaching pada siswa kelas V SD Negeri 29 Pekanbaru. Penelitian ini dilaksanakan sebanyak dua siklus dan setiap siklus terdiri dari dua kali pertemuan kegiatan pembelajaran dan satu kali ulangan harian. Hasil penelitian menunjukkan bahwa penerapan model Quantum Teaching dapat meningkatkan hasil belajar IPS. Hal ini terbukti nilai rata-rata yang diperoleh siswa setiap siklus mengalami peningkatan. Dari skor dasar ke ulangan harian I yaitu rata-rata 55.35 menjadi 74.59. Lalu ulangan harian II meningkat menjadi 82.02. Aktivitas guru dalam penerapan model Quantum Teaching mengalami peningkatan pada siklus I pertemuan I 62.5% meningkat pertemuan II menjadi 79.16%, lalu pada siklus II pertemuan I 83.33% meningkat pertemuan II menjadi 91.66%. Aktivitas siswa dalam penerapan model Quantum Teaching mengalami peningkatan pada siklus I pertemuan I 58.33% meningkat pertemuan II menjadi 62.5%, lalu pada siklus II pertemuan I 79.16% meningkat pertemuan II menjadi 87.5%. Hasil penelitian di kelas V SD Negeri 29 Pekanbaru membuktikan bahwa penerapan model Quantum Teaching dapat meningkatkan hasil belajar IPS.
IMPROVING LANGUAGE SKILLS FOR EARLY CHILDHOOD
Authors: Chairilsyah Daviq
Published: March 2022

Language skills are very important in early childhood development. This literature review aims to provide reinforcement to parents and teachers in order to stimulate language skills in children from an early age. It is necessary applied for children so that the opportunity to make the children’s golden age of 0 to 4 can be realized on both aspects of development, especially in the language improvement. Language improvement in early childhood is not only oriented to the ability to speak alone but also a process that successively starts from listening, speaking, reading, and writing. This is important for children to be improved and optimized since kindergarten, subsequently when taking formal education in elementary, junior, senior high school, and college, children's language skills are in a good category. Meanwhile, these skills are also essential for a career in the future. Parents as the child's closest environment must stimulate and adjust children to speak properly so that language development can be optimal. In addition, teachers also have a very big role in stimulating children through storytelling, question and answer methods, dialogue methods, and many more methods that can be used in stimulating the language development of their students at school.
POTRET INTERAKSI SOSIAL DALAM ANTOLOGI CERPEN INSPIRATIF 18 CERITA MENGGUGAH
Authors: Haryawati Siti
Published: March 2022

Interaksi sosial tidak dapat dihindari dalam kehidupan bermasyarakat. Melalui interaksi, manusia dapat bekerjasama, sehingga proses pencapaian tujuan hidup individu atau kelompok lebih mudah terwujud. Interaksi juga mendorong terwujudnya pola kehidupan individu atau kelompok secara integratif, sehingga setiap individu dapat meningkatkan kualitas beragam peran sosial dalam kehidupan kelompok. Antologi Cerpen Inspiratif “18 Cerita Menggugah” merupakan kumpulan cerita yang dapat memberikan inspirasi kepada pembacanya dari berbagai cabang kehidupan. Buku ini sangat relevan dengan kurikulum 2013 yang mengedepankan pembalajaran teks-teks cerita yang menginspirasi yang memuat nilai-nilai sosial dan budaya,  religius, dan nilai moral. Karya sastra memiliki berbagai kelebihan yang tidak dimiliki oleh sumber belajar lainnya. Penelitian ini bertujuan untuk mendeskripsikan interaksi sosial dalam Antologi cerpen inspiratif ”18 Cerita Menggungah” dengan mengkhususkan kajian terhadap dua cerpen yaitu “Perjalanan Hidup”, dan “Kitty, Si Penyuka Boneka ”. Metode yang digunakan dalam penelitian ini adalah kualitatif dengan mengunakan pendekatan sosiologi sastra yang menitik beratkan kajiannya pada karya sastra. Berdasarkan hasil analisis, ditemukan lima interaksi sosial asosiatif;  dua bentuk akomodasi, dua bentuk kerja sama, satu bentuk asimilasi sedangkan analisis dua interaksi sosial disosiatif;dua bentuk pertikaian dan satu bentuk kontravensi. Serta dua kutipan yang menggambarkan adanya nilai kerohanian dalam berinteraksi.

Kata Kunci: Interaksi Sosial, Cerpen Inspiratif, 18 Cerita Menggugah
ANALISIS KEBUTUHAN GURU DALAM PENGEMBANGAN BUKU AJAR BERMUATAN KEARIFAN LOKAL PASCA PANDEMI CIVID-19 DI KABUPATEN KOTABARU
Authors: Ali Muhammad , Mubarak Husni , DP Usman
Published: March 2022

The aims of this research to analysis needs in development of teaching materials containing local of wisdom that the appropriate with characteristic existing of students. The research is descriptive qualitative of research with using design the case study of the research. The research of sample 20 teacher to every school that are in Kabupaten Kotabaru. The technique of collecting data that used of questioner and the analysis needs in development of teaching materials containing local of wisdom and development based on the analysis of indicator. The data obtained and then the analysis using the descriptive based on the result of the total answer that giving the respondent. The result of analysis questioner needs and the result observation development of teaching book show that the teacher and students need textbooks containing post covid-19 local wisdom in Indonesia that are easy to understand, equipped with pictures that support of learning materials, containing the latest findings related to learning materials, and there is a glossary containing terms in the field of learning.

 

Key words: Need analysis, teaching book, teacher needs.
ANALISIS MOTIVASI DAN HASIL BELAJAR MATEMATIKA TERHADAP PEMBELAJARAN DARING PADA PESERTA DIDIK KELAS VII SMPN 5 KOTABARU
Authors: Aprida Muli , Ali Muhammad
Published: March 2022

This study aims to determine the motivation and learning outcomes of students in class VII SMPN 5 Kotabaru after the application of online learning. The method used in this research is descriptive research with a qualitative approach that utilizes qualitative data and is described descriptively. Descriptive research aims to create a systematic, factual and accurate description, description of the facts, properties and relationship between the phenomena be`ing investigated. The results showed that the learning motivation of class VII students at SMPN 5 Kotabaru was categorized as high with an average score of 77% ant the learning outcomes of grade VII students at SMPN 5 Kotabaru after online learning could be categorized as good with an average 69,17. Motivations to learn is not the main factor in determining learning outcomes, but learning motivation is important for increasing optimal learning outcomes.

 

Keywords: Learning Motivation, Mathematics Learning Outcomes, Online Learning.
Analisis Tekstual Puisi Cinta Itu, Kamu karya Moammar Emka
Authors: Normasunah Normasunah
Published: March 2022

Penelitian ini menggunakan metode kualitatif  deskriptif. pengumpulan data dilakukan teknik pustaka, pengumpulan data berdasarkan sumber-sumber tertulis dan teknik catat, mengadakan pencatatan  terhadap data relevan  sesuai sasaran dan tujuan penelitian. Sumber data adalah buku Cinta Itu, Kamu karya Moammar Emka. Hasil analisis dapat disimpulkan bahwa puisi dalam buku Cinta Itu, Kamu karya Moammar Emka terdapat sarana gramatikal dan sarana leksikal yang membangun teks. (1) Sarana gramatikal terdiri atas : Referensi (pengacuan) yang terbagi menjadi tiga, yaitu referensi persona, referensi demonstratif dan referensi komparatif . Subtitusi  ditandai  adanya pergantian satuan lingual tertentu dengan satuan lingual lain. Elipsis ditandai adanya unsur yang dihilangkan, tetapi telah disebutkan terlebih dahulu. Konjungsi terbagi menjadi konjungsi koodinatif, konjungsi subordinatif, konjungsi antarkalimat, dan konjungsi korelatif. (2) Sarana leksikal terdiri atas : Repetisi terbagi menjadi tiga macam yaitu : repetisi penuh, repetisi dengan perubahan bentuk, dan repetisi parsial. Sinonim yang ditandai dengan adanya persamaan kata antara morfem bebas dengan morfem terikat,  kata dengan kata, kata dengan frasa atau sebaliknya. Antonim yang ditandai dengan adanya antonim mutlak dan antonim hieralkial. Kolokasi ditandai hadirnya kata-kata yang cenderung dipakai secara bersamaan. Hiponim ditandai  kata-kata yang terwakili oleh hipernim yang menjadi superordinat dari penyebutan kata-kata lainnya.

 

Kata Kunci: Analisis tekstual, puisi
MENGGUNAKAN METODE PARTISIPATORI DENGAN TEKNIK ATTL (AMATI, TANYA, TULIS, LAPORKAN) DALAM UPAYA MENINGKATKAN KETERAMPILAN MENULIS TEKS BERITA PADA PESERTA DIDIK KELAS VIIIA MTs NEGERI 1 KOTABARU TAHUN PELAJARAN 2018/2019
Authors: Muin Ibrahim
Published: March 2022

penelitian ini bertujuan untuk (1) Untuk mendeskripsikan peningkatan keterampilan menulis teks berita pada Peserta didik kelas VIIIA MTs Negeri 1 Kotabaru setelah mengikuti pembelajaran dengan metode partisipatori menggunakan teknik ATTL, (2) Untuk mendeskripsikan perubahan perilaku Peserta didik kelas VIIIA MTs Negeri 1 Kotabaru setelah mengikuti pembelajaran dengan metode partisipatori menggunakan teknik ATTL.

Metode yang digunakan yaitu metode pastisipatori dengan teknik ATTL. Penelitian ini dilaksanakan dalam dua tahap, yaitu siklus I dan siklus II. Pengumpulan data pada siklus I dan siklus II menggunakan teknik tes dan nontes. Teknik nontes berupa observasi, dan wawancara. Teknik analisis data dilakukan secara kuantitatif dan kualitatif.

Hasil ketuntasan tes pada siklus I sebesar 6,25% rata-rata sebesar 36,46 kategori kurang. Pada siklus II, ketuntasan Peserta Didik mencapai 100% rata-rata 97,15 kategori sangat baik. Terjadi peningkatan dari siklus I sebesar 93,75%. Pembelajaran menulis teks berita menggunakan metode pastisipatori dengan teknik ATTL mampu mengubah perilaku Peserta Didik kelas VIIIA MTs Negeri 1 Kotabaru ke arah yang positif. Hal tersebut dapat diketahui dari hasil nontes yang meliputi hasil observasi, dan  wawancara pada siklus I dan siklus II.

 

Kata Kunci: Metode Partisipatori, Teknik ATTL, Katerampilan Menulis Teks Berita.
ANALISIS SEMIOTIKA DALAM KUMPULAN CERPEN PELANGI DI UJUNG SENJA KARYA RATIH AYUNINGRUM
Authors: Juniati Sri
Published: March 2022

The aims of this research are  To describe semiotics in the collection of short stories Pelangi di Ujung Senja by Ratih Ayuningrum. Describe the dominance of semiotics in the collection of short stories Pelangi di Ujung Senja by Ratih Ayuningrum.

The method used is a descriptive qualitative research method that describes semiotics in the collection of short stories Pelangi di Ujung Senja by Ratih Ayungingrum. Researchers as a key instrument. The collection of short stories Pelangi di Ujung Senja by Ratih Ayungrum as primary data and secondary data are books related to semiotic theory, literary theories, research methods and other books as supporting research. 

The results of the research on the collection of short stories Pelangi di Ujung Senja by Ratih Ayuningrum, showed that Semiotics in the collection of short stories Pelangi di Ujung Senja contained four, namely index, sign, icon and gesture. While the type of semiotics that dominates in the collection of short stories Pelangi di Ujung Senja by Ratih Ayuningrum is the index type, with a total of 44 citations.

 

Kata Kunci: Semiotika, Cerpen.
Israel’s ‘Nationality Law’: Reconsidering Settlement, Citizenship and Ethics in the Context of Occupation
Authors: Chowers Eyal
Published: March 2022

In 2018, the State of Israel enacted a new constitutional law: ‘BASIC LAW: ISRAEL-THE NATION STATE OF THE JEWISH PEOPLE’. The Law reflects diverse Zionist ideologies which were nevertheless all ‘land-centered’ rather than state-centered from an early stage; it reformulates that intellectual tradition, however, promoting Jewish settlements in conditions of occupation and celebrates, for the first time, settlement as the prime and exclusive goal of the state. Partly to facilitate this goal, and to further blur borders, the law also contracts the meaning and status of Palestinians’ citizenship in Israel, thus at least symbolically narrowing the (still significant) political-legal gap between these citizens and the Palestinians living in the West Bank. Finally, the Law seems to vacate the state from its ethical dimension and commitments as defined by its Declaration of independence (1948) — including its commitments to the democratic principles of political liberty for all and equality — thus manifesting the influence ruling and subjugating others through military government is having on Israel’s constitutional framework.
Performance Monitoring of Anaerobic Digestion at Various Organic Loading Rates of Commercial Malaysian Food Waste
Authors: Zainal Afifi , Harun Razif , Idrus Syazwani
Published: March 2022

Application of anaerobic digestion (AD) has become common in treating palm oil mill effluent in Malaysia; however, employing AD in treating the organic fraction of municipal solid waste (OFMSW), especially food waste, is still scarce. This study aims to characterize the commercial Malaysian food waste (CMFW) and determine its potential as sustainable bioenergy feedstock through biogas production. The sample was digested via the biomethane potential (BMP) test with the variation of organic loading rates (OLRs), ranging from 0.38 to 3.83 gCOD/L. day, under mesophilic conditions. The digestion process was further evaluated in continuous operation using a 6-L continuous stirred-tank reactor (CSTR). The kinetic properties of the process were also determined. It was found that the CMFW had a significant amount of chemical oxygen demand of 230 g/L and an acidic pH of 4.5 with the carbon to nitrogen (C/N) ratio at 121:1. A maximum methane composition of 81% was obtained at 1.92 gCOD/L in the BMP test with specific methane production (SMP) at 0.952 L. CH4/L.COD fed. The biogas production was well-fitted with the modified Gompertz model with R2 at 0.9983 and the maximum biogas potential production rate at Rm 0.1573 L/day, whereas in the CSTR operation, a maximum methane composition of 85% was produced at OLR 6 gCOD/L. day with the SMP of 1.13 L. CH4/L.COD fed. The CSTR system was in high stability as the pH was maintained in a range of 6.6–6.7, with an alkalinity ratio of 0.28. This study indicates the CMFW is a sustainable feedstock for biogas production in Malaysia. Toward a circular economy approach, the authorities shall introduce commercial scale CMFW AD as part of managing municipal solid waste issues in Malaysia.
Design and Optimization of a New Anti-reflux Biliary Stent With Retractable Bionic Valve Based on Fluid-Structure Interaction Analysis
Authors: Su Yushan , Xiang Zhongxia , Song Xiaofei , Zheng Shuxian , Xu Xinsheng
Published: March 2022

Duodenal biliary reflux has been a challenging common problem which could cause dreadful complications after biliary stent implantation. A novel anti-reflux biliary stent with a retractable bionic valve was proposed according to the concertina motion characteristics of annelids. A 2D equivalent fluid-structure interaction (FSI) model based on the axial section was established to analyze and evaluate the mechanical performances of the anti-reflux biliary stent. Based on this model, four key parameters (initial shear modulus of material, thickness, pitch, and width) were selected to investigate the influence of design parameters on anti-reflux performance via an orthogonal design to optimize the stent. The results of FSI analysis showed that the retrograde closure ratio of the retractable valve primarily depended on initial shear modulus of material (p < 0.05) but not mainly depended on the thickness, pitch, and width of the valve (p > 0.05). The optimal structure of the valve was finally proposed with a high retrograde closing ratio of 95.89%. The finite element model revealed that the optimized anti-reflux stent possessed improved radial mechanical performance and nearly equal flexibility compared with the ordinary stent without a valve. Both the FSI model and experimental measurement indicated that the newly designed stent had superior anti-reflux performance, effectively preventing the duodenobiliary reflux while enabling the bile to pass smoothly. In addition, the developed 2D equivalent FSI model provides tremendous significance for resolving the fluid-structure coupled problem of evolution solid with large deformation and markedly shortens the calculation time.
A Portable Droplet Magnetofluidic Device for Point-of-Care Detection of Multidrug-Resistant Candida auris
Authors: Lee Pei-Wei , Totten Marissa , Chen Liben , Chen Fan-En , Trick Alexander Y. , Shah Kushagra , Ngo Hoan Thanh , Jin Mei , Hsieh Kuangwen , Zhang Sean X. , Wang Tza-Huei
Published: March 2022

Candida auris is an emerging multidrug-resistant fungal pathogen that can cause severe and deadly infections. To date, C. auris has spurred outbreaks in healthcare settings in thirty-three countries across five continents. To control and potentially prevent its spread, there is an urgent need for point-of-care (POC) diagnostics that can rapidly screen patients, close patient contacts, and surveil environmental sources. Droplet magnetofluidics (DM), which leverages nucleic acid-binding magnetic beads for realizing POC-amenable nucleic acid detection platforms, offers a promising solution. Herein, we report the first DM device—coined POC.auris—for POC detection of C. auris. As part of POC.auris, we have incorporated a handheld cell lysis module that lyses C. auris cells with 2 min hands-on time. Subsequently, within the palm-sized and automated DM device, C. auris and control DNA are magnetically extracted and purified by a motorized magnetic arm and finally amplified via a duplex real-time quantitative PCR assay by a miniaturized rapid PCR module and a miniaturized fluorescence detector—all in ≤30 min. For demonstration, we use POC.auris to detect C. auris isolates from 3 major clades, with no cross reactivity against other Candida species and a limit of detection of ∼300 colony forming units per mL. Taken together, POC.auris presents a potentially useful tool for combating C. auris.
Extracellular Vesicles From Microalgae: Uptake Studies in Human Cells and Caenorhabditis elegans
Authors: Picciotto Sabrina , Santonicola Pamela , Paterna Angela , Rao Estella , Raccosta Samuele , Romancino Daniele Paolo , Noto Rosina , Touzet Nicolas , Manno Mauro , Di Schiavi Elia , Bongiovanni Antonella , Adamo Giorgia
Published: March 2022

Extracellular vesicles (EVs) are lipid membrane nano-sized vesicles secreted by various cell types for intercellular communication, found in all kingdoms of life. Nanoalgosomes are a subtype of EVs derived from microalgae with a sustainable biotechnological potential. To explore the uptake, distribution and persistence of nanoalgosomes in cells and living organisms, we separated them from a culture of the chlorophyte Tetraselmis chuii cells by tangential flow filtration (TFF), labelled them with different lipophilic dyes and characterized their biophysical attributes. Then we studied the cellular uptake of labelled nanoalgosomes in human cells and in C. elegans, demonstrating that they enter the cells through an energy dependent mechanism and are localized in the cytoplasm of specific cells, where they persist for days. Our data confirm that nanoalgosomes are actively uptaken in vitro by human cells and in vivo by C. elegans cells, supporting their exploitation as potential nanocarriers of bioactive compounds for theranostic applications.
Development and Validation of a New Anisotropic Visco-Hyperelastic Human Head Finite Element Model Capable of Predicting Multiple Brain Injuries
Authors: Lyu Ding , Zhou Runzhou , Lin Chin-hsu , Prasad Priya , Zhang Liying
Published: March 2022

This paper reports on the latest refinement of the Finite Element Global Human Body Models Consortium 50th percentile (GHBMC M50) adult male head model by the development and incorporation of a new material model into the white matter tissue of the brain. The white matter is represented by an anisotropic visco-hyperelastic material model capable of simulating direction-dependent response of the brain tissue to further improve the bio-fidelity and injury predictive capability of the model. The parameters representing the material were optimized by comparing model responses to seven experimentally reported strain responses of brains of postmortem human subjects (PMHS) subjected to head impact. The head model was subjected to rigorous validation against experimental data on force–deflection responses in the skull and face, intracranial pressure, and brain strain responses from over 34 PMHS head impact experiments. Crash-induced injury indices (CIIs) for facial bone fracture, skull fracture, cerebral contusion, acute subdural hematomas (ASDHs), and diffuse brain injury were developed by reconstructing 32 PMHS and real-world injury cases with the model. Model predicted maximum principal strain (MPS) and stress were determined as fracture CIIs for compact bone and spongy bones, respectively, in the skull and face. Brain responses in terms of MPS, MPS rates, and pressure distribution in injury producing experimental impacts were determined using the model and analyzed with logistic regression and survival analysis to develop CIIs for brain contusions, diffuse brain injuries, and ASDH. The statistical models using logistic regression and survival analysis showed high accuracy with area under the receiver operating curve greater than 0.8. Because of lack of sufficient moderate diffuse brain injury data, a statistical model was not created, but all indications are that the MPS rate is an essential brain response that discriminates between moderate and severe brain injuries. The authors stated that the current GHBMC M50 v.6.0 is an advanced tool for injury prediction and mitigation of injuries in automotive crashes, sports, recreational, and military environments.
Evaluation of Computational Methodologies for Accurate Prediction of Wall Shear Stress and Turbulence Parameters in a Patient-Specific Aorta
Authors: Manchester Emily Louise , Pirola Selene , Salmasi Mohammad Yousuf , O’Regan Declan P. , Athanasiou Thanos , Xu Xiao Yun
Published: March 2022

Background: Recent studies suggest that blood flow in main arteries is intrinsically disturbed, even under healthy conditions. Despite this, many computational fluid dynamics (CFD) analyses of aortic haemodynamics make the assumption of laminar flow, and best practices surrounding appropriate modelling choices are lacking. This study aims to address this gap by evaluating different modelling and post-processing approaches in simulations of a patient-specific aorta.

Methods: Magnetic resonance imaging (MRI) and 4D flow MRI from a patient with aortic valve stenosis were used to reconstruct the aortic geometry and derive patient-specific inlet and outlet boundary conditions. Three different computational approaches were considered based on assumed laminar or assumed disturbed flow states including low-resolution laminar (LR-Laminar), high-resolution laminar (HR-Laminar) and large-eddy simulation (LES). Each simulation was ran for 30 cardiac cycles and post-processing was conducted on either the final cardiac cycle, or using a phase-averaged approach which utilised all 30 simulated cycles. Model capabilities were evaluated in terms of mean and turbulence-based parameters.

Results: All simulation types, regardless of post-processing approach could correctly predict velocity values and flow patterns throughout the aorta. Lower resolution simulations could not accurately predict gradient-derived parameters including wall shear stress and viscous energy loss (largest differences up to 44.6% and 130.3%, respectively), although phase-averaging these parameters improved predictions. The HR-Laminar simulation produced more comparable results to LES with largest differences in wall shear stress and viscous energy loss parameters up to 5.1% and 11.6%, respectively. Laminar-based parameters were better estimated than turbulence-based parameters.

Conclusion: Our findings suggest that well-resolved laminar simulations can accurately predict many laminar-based parameters in disturbed flows, but there is no clear benefit to running a HR-Laminar simulation over an LES simulation based on their comparable computational cost. Additionally, post-processing “typical” laminar simulation results with a phase-averaged approach is a simple and cost-effective way to improve accuracy of lower-resolution simulation results.
Is Training With Gym Machines Safe After Hip Arthroplasty?—An In Vivo Load Investigation
Authors: Haffer Henryk , Bender Alwina , Krump Alexander , Hardt Sebastian , Winkler Tobias , Damm Philipp
Published: March 2022

Background: Training with gym machines is one of the most popular physical activities after total hip arthroplasty (THA). However, to date, there are no evidence-based recommendations for physical activity after THA, worldwide. The aim of the study is to evaluate the in vivo hip joint loads during exercises on four widely used gym machines in order to provide a source for an evidence-based patient counselling for arthroplasty surgeons.

Methods: The in vivo hip joint loads in seven patients (59.6 ± 6.4 years, 28.6 ± 2.1 kg/m2) with instrumented hip implants were assessed. The resulting force (Fres), bending moment (Mbend), and torsional moment (Mtors) were evaluated during the training on leg curl/leg extension machines (loads: 20, 30, and 40 kg), leg press machine [backrest: 10°, 30°, and 60°; load: 50, 75, and 100%BW (bodyweight)], and a rope pull machine (abduction/adduction/flexion/extension; each ipsi- and contralateral; load 10 kg). These loads were compared with the loads during walking on treadmill at 4 km/h (median peak values: Fres 303%BW, Mbend 4.25%BWm, and Mtors 2.70%BWm).

Results: In each of the four performed exercises with a total of 23 different load conditions or exercise modes analyzed, a significantly lower or not different load was detected with respect to Fres, Mbend, and Mtors measured while walking with 4 km/h. Nevertheless, Fres and Mbend demonstrated a trend to increased loading during the ipsilateral monopod standing rope pull exercises hip flexion, extension, and abduction.

Conclusion: Based on our investigation, we assume that the investigated gym machines and external loads can be considered mainly as low-impact sports (with some exceptions) and thus as safe physical activity after THA. Due to the fact that the examinations were conducted in the mean 17.4 months after THA, the applicability of the results to the immediate postoperative period is limited.
High Sensitivity, Rapid Detection of Virus in High Traffic Environments
Authors: Waller Lauren , Guo Zhilin , Tang Rui , Zhang Zunming , Wang Edward , Yasuhara-Bell Jarred , Laurent Louise , Lo Yu-Hwa
Published: March 2022

The global pandemic caused by the SARS-CoV-2 virus has underscored the need for rapid, simple, scalable, and high-throughput multiplex diagnostics in non-laboratory settings. Here we demonstrate a multiplex reverse-transcription loop-mediated isothermal amplification (RT-LAMP) coupled with a gold nanoparticle-based lateral flow immunoassay (LFIA) capable of detecting up to three unique viral gene targets in 15 min. RT-LAMP primers associated with three separate gene targets from the SARS-CoV-2 virus (Orf1ab, Envelope, and Nucleocapsid) were added to a one-pot mix. A colorimetric change from red to yellow occurs in the presence of a positive sample. Positive samples are run through a LFIA to achieve specificity on a multiplex three-test line paper assay. Positive results are indicated by a characteristic crimson line. The device is almost fully automated and is deployable in any community setting with a power source.
Vimentin Suppresses Inflammation and Tumorigenesis in the Mouse Intestine
Authors: Wang Linglu , Mohanasundaram Ponnuswamy , Lindström Michelle , Asghar Muhammad Nadeem , Sultana Giulia , Misiorek Julia O. , Jiu Yaming , Chen Hongbo , Chen Zhi , Toivola Diana M. , Cheng Fang , Eriksson John E.
Published: March 2022

Vimentin has been implicated in wound healing, inflammation, and cancer, but its functional contribution to intestinal diseases is poorly understood. To study how vimentin is involved during tissue injury and repair of simple epithelium, we induced colonic epithelial cell damage in the vimentin null (Vim−/−) mouse model. Vim−/− mice challenged with dextran sodium sulfate (DSS) had worse colitis manifestations than wild-type (WT) mice. Vim−/− colons also produced more reactive oxygen and nitrogen species, possibly contributing to the pathogenesis of gut inflammation and tumorigenesis than in WT mice. We subsequently describe that CD11b+ macrophages served as the mainly cellular source of reactive oxygen species (ROS) production via vimentin-ROS-pSTAT3–interleukin-6 inflammatory pathways. Further, we demonstrated that Vim−/− mice did not develop colitis-associated cancer model upon DSS treatment spontaneously but increased tumor numbers and size in the distal colon in the azoxymethane/DSS model comparing with WT mice. Thus, vimentin has a crucial role in protection from colitis induction and tumorigenesis of the colon.
Trypanosoma cruzi Genomic Variability: Array Comparative Genomic Hybridization Analysis of Clone and Parental Strain
Authors: Cortez Danielle Rodrigues , Lima Fabio Mitsuo , Reis-Cunha João Luís , Bartholomeu Daniella Castanheira , Villacis Rolando Andre Rios , Rogatto Silvia Regina , Costa-Martins André Guilherme , Marchiano Fernanda Sycko , do Carmo Rafaela Andrade , da Silveira Jose Franco , Marini Marjorie Mendes
Published: March 2022

Trypanosoma cruzi, the etiological agent of Chagas disease, exhibits extensive inter- and intrastrain genetic diversity. As we have previously described, there are some genetic differences between the parental G strain and its clone D11, which was isolated by the limiting dilution method and infection of cultured mammalian cells. Electrophoretic karyotyping and Southern blot hybridization of chromosomal bands with specific markers revealed chromosome length polymorphisms of small size with additional chromosomal bands in clone D11 and the maintenance of large syntenic groups. Both G strain and clone D11 belong to the T. cruzi lineage TcI. Here, we designed intraspecific array-based comparative genomic hybridization (aCGH) to identify chromosomal regions harboring copy-number variations between clone D11 and the G strain. DNA losses were more extensive than DNA gains in clone D11. Most alterations were flanked by repeated sequences from multigene families that could be involved in the duplication and deletion events. Several rearrangements were detected by chromoblot hybridization and confirmed by aCGH. We have integrated the information of genomic sequence data obtained by aCGH to the electrophoretic karyotype, allowing the reconstruction of possible recombination events that could have generated the karyotype of clone D11. These rearrangements may be explained by unequal crossing over between sister or homologous chromatids mediated by flanking repeated sequences and unequal homologous recombination via break-induced replication. The genomic changes detected by aCGH suggest the presence of a dynamic genome that responds to environmental stress by varying the number of gene copies and generating segmental aneuploidy.
Mixed Vaginitis in the Third Trimester of Pregnancy Is Associated With Adverse Pregnancy Outcomes: A Cross-Sectional Study
Authors: Li Huanrong , Dong Mengting , Xie Wenjuan , Qi Wenhui , Teng Fei , Li Huiyang , Yan Ye , Wang Chen , Han Cha , Xue Fengxia
Published: March 2022

Mixed vaginitis is a complex vaginal dysbiosis that differs from single vaginitis. Vaginitis in the third trimester may lead to adverse maternal and neonatal outcomes. The clinical characteristics, microbiological characteristics, and adverse pregnancy outcomes of mixed vaginitis in late pregnancy are worth studying. Therefore, this study investigated the clinical and microbiological characteristics of vaginitis and adverse pregnancy outcomes of patients with mixed vaginitis. We studied 1,674 women in late pregnancy who attended the Tianjin Medical University General Hospital from November, 2019 to October, 2021. We administered standardized questionnaires, performed vaginal examination and sampling plus microscope examinations, and assessed follow-up pregnancy outcomes. We cultured the vaginal discharge of the patients with mixed vaginitis to isolate pathogens and performed antimicrobial susceptibility tests of the isolated pathogens. For the patients with peripartum infection, we collected a sample to isolate pathogens. Among the 1,674 women, 66 (3.9%) had mixed vaginitis. The independent risk factor for mixed vaginitis in late pregnancy was a history of vaginitis during early and middle pregnancy (OR = 5.637, 95% CI: 3.314–9.580). The signs of vaginal erythema (63.6% vs. 42.0%), yellow discharge (81.8% vs. 59.6%), and malodor (31.8% vs. 18.8%) (P <0.05) were significantly higher in patients with mixed vaginitis than in patients with single vaginitis. Bacterial isolates of the vaginal secretions of patients with mixed bacterial vaginitis were mainly the pathogens of aerobic vaginitis and bacterial vaginosis, such as Gardnerella vaginalis, Streptococcus anginosus, and Staphylococcus epidermidis. Pathogen isolation of the vaginal secretions of patients with mixed fungus and bacteria vaginitis mainly included Candida albicans, followed by S. anginosus, Enterococcus faecalis, Staphylococcus hemolyticus, Staphylococcus aureus, Streptococcus agalactiae and Staphylococcus simulans. Women with mixed vaginitis had an increased incidence and risk of peripartum infections (6.1% vs. 1.4%, P <0.05; OR = 3.985, 95% CI:1.214–13.079). Escherichia coli is the main pathogen that causes peripartum infection. Mixed vaginitis in late pregnancy is characterized by a severe and complex phenotype, complex vaginal dysbiosis, and a long course of vaginal dysbiosis. This can lead to an increased incidence and risk of peripartum infection. Therefore, more attention should be paid to patients with mixed vaginitis in the third trimester of pregnancy.
Probiotic Species in the Management of Periodontal Diseases: An Overview
Authors: Zhang Yuwei , Ding Yi , Guo Qiang
Published: March 2022

Periodontal diseases are one of the most common chronic inflammatory diseases of the oral cavity, which are initiated and sustained by pathogenic plaque biofilms. Central to modern periodontology is the idea that dysbiosis of periodontal microecology and disorder of host inflammatory response gives rise to degradation of periodontal tissues together, which eventually leads to tooth loss, seriously affecting the life quality of patients. Probiotics were originally used to treat intestinal diseases, while in recent years, extensive studies have been exploring the utilization of probiotics in oral disease treatment and oral healthcare. Probiotic bacteria derived from the genera Lactobacillus, Bifidobacterium, Streptococcus, and Weissella are found to play an effective role in the prevention and treatment of periodontal diseases via regulating periodontal microbiota or host immune responses. Here, we review the research status of periodontal health-promoting probiotic species and their regulatory effects. The current issues on the effectiveness and safety of probiotics in the management of periodontal diseases are also discussed at last. Taken together, the use of probiotics is a promising approach to prevent and treat periodontal diseases. Nevertheless, their practical use for periodontal health needs further research and exploration.
Elucidation of the Reinforcing Spleen Effect of Jujube Fruits Based on Metabolomics and Intestinal Flora Analysis
Authors: Yi Yan-ling , Li Yao , Guo Sheng , Yan Hui , Ma Xin-fei , Tao Wei-wei , Shang Er-xin , Niu Yang , Qian Da-wei , Duan Jin-ao
Published: March 2022

Jujube (Ziziphus jujuba Mill.) fruit (JF) is widely consumed as food in Asian countries due to its potential effects for human health. As a traditional Chinese medicine, JF is often used to treat anorexia, fatigue and loose stools caused by spleen deficiency syndromes in China, but the mechanism underlying this effect has not been thoroughly elucidated. In this study, a rat model of spleen deficiency syndromes was adopted to investigate the therapeutic effect of JF extract and its possible mechanism by metabolomics analyses of plasma and urine as well as the intestinal flora analysis. The results showed that the changes in plasma and urine metabolites caused by spleen deficiency were reversed after administration of JF, and these changed endogenous metabolites were mainly involved in retinol metabolism, pentose and glucuronate interconversions, nicotinate and niacinamide metabolism pathways. The 16S rDNA sequencing results showed that JF could regulate intestinal flora imbalance caused by spleen deficiency. The covariance analysis of intestinal flora structure and metabolome indicated that Aerococcus may be a candidate strain for predicting and treating the metabolic pathways of spleen deficiency and related disorders. In summary, it can be revealed that spleen deficiency, which alters metabolic profiles and the intestinal flora, could be alleviated effectively by JF extract.
The Influence of Blue Light and the BlsA Photoreceptor on the Oxidative Stress Resistance Mechanisms of Acinetobacter baumannii
Authors: Squire Mariah S. , Townsend Hope A. , Actis Luis A.
Published: March 2022

Acinetobacter baumannii is a catalase-positive Gram-negative bacterial pathogen that causes severe infections among compromised patients. Among its noteworthy regulatory mechanisms, this microorganism regulates its lifestyle through the blue light using flavin (BLUF) protein BlsA. This protein regulates a diverse set of cellular processes that include, but are not limited to, motility, biofilm formation, phenylacetic acid metabolism, iron uptake, and catalase activity. We set out to determine how A. baumannii regulates catalase activity and other related oxidative stress phenotypes in response to light. Notably, because A. baumannii ATCC 17978 encodes four catalase homologs – which we refer to as KatA, KatE, KatE2, and KatG – we also aimed to show which of these enzymes exhibit light- and BlsA-dependent activity. Our work not only provides insight into the general function of all four catalase homologs and the impact of light on these functions, but also directly identifies KatE as a BlsA-regulated enzyme. We further demonstrate that the regulation of KatE by BlsA is dependent on a lysine residue that we previously demonstrated to be necessary for the regulation of surface motility. Furthermore, we show that BlsA’s five most-C-terminal residues – previously considered dispensable for BlsA’s overall function – are necessary for the light-independent and light-dependent regulation of catalase and superoxide dismutase activities, respectively. We hypothesize that these identified critical residues are necessary for BlsA’s interaction with protein partners including the transcriptional regulators Fur and BfmR. Together these data expand the understanding regarding how A. baumannii uses light as a signal to control oxidative stress resistance mechanisms that are critical for its pathophysiology.
Research Progress on the Correlation Between Gardnerella Typing and Bacterial Vaginosis
Authors: Qin Hanyu , Xiao Bingbing
Published: March 2022

Bacterial vaginosis (BV) is the most common infectious disease of the reproductive tract in women of childbearing age. It often manifests as an imbalance in the vaginal microbiome, including a decrease in Lactobacillus and an increase in anaerobic bacteria. While Gardnerella spp. are considered a major cause of BV, they are also detected in the vaginal microbiome of healthy women. G. vaginalis was the only recognized species of Gardnerella until a recent study characterized three new species, G. leopoldii, G. piotii, and G. swidsinskii. This review describes the different types and genetic diversity of Gardnerella, as well as new findings on the correlation between different Gardnerella spp. and BV.
Evaluation of Simpler Criteria for Diagnosing Allergic Bronchopulmonary Aspergillosis Complicating Asthma
Authors: Agarwal Ritesh , Saxena Puneet , Muthu Valliappan , Sehgal Inderpaul Singh , Dhooria Sahajal , Prasad Kuruswamy Thurai , Aggarwal Ashutosh Nath , Chakrabarti Arunaloke
Published: March 2022

Background

The modified International Society for Human and Animal Mycology (ISHAM) allergic bronchopulmonary aspergillosis (ABPA) working group (AWG) criteria lists up to five components for diagnosing ABPA in asthmatics. Whether eliminating specific components of the existing criteria would have the same diagnostic utility as the original remains unknown.

Objective

To evaluate the performance of several simplified criteria for diagnosing ABPA.

Methods

We compared the performance of seven new criteria (after excluding or modifying one or more of the components of the ISHAM-AWG criteria) with the modified ISHAM-AWG criteria in asthmatic subjects using latent class analysis (LCA). We also tested the performance of the newer criteria using accuracy measures against a multidisciplinary team (MDT) diagnosis of ABPA. We considered the diagnostic accuracy of the newer criteria to be acceptable if the correct classification and false-negative rates were >95% and <5%, respectively, on an MDT evaluation.

Results

We analyzed data from 543 asthmatic subjects (58.8% women; mean age, 36.8 years). Using LCA, the sensitivity of the A.fumigatus-specific IgE-based criteria ranged from 92-99%, while the specificity varied between 92% and 100%. The MDT diagnosed ABPA in 106 (19.5%) subjects. Using MDT as the reference standard, the correct classification and false-negative rates were >95% for three of the seven and <5% for four of the seven newer criteria.

Conclusions

We found several of the newly developed criteria to perform, like the modified ISHAM-AWG criteria, for diagnosing ABPA complicating asthma. A prospective study in current clinical algorithms is required for validating our observations.