bluuebunny/crossref_metadata_2025 huggingface dataset:
Invertase Plays a Vital Role in Regulating Wound-Induced Accumulation of Phenolic Compounds in Carrot Tissue
Authors: Ren Xinle , Li Meilin , Zhang Meixue , Zhao Ruping , Han Cong
Published: January 2024

The application of wounding stress can induce the accumulation of phenolic antioxidants in carrots. This study aimed to investigate the possible regulation role of invertase (INV) on the biosynthesis of phenolics in wounded carrots. In this study, carrots were cut into two different wounding intensities of slices and cubes, then stored at 20 °C for 2 days. The results showed that wounding stress caused an obvious increase in phenolic content and antioxidant capacity in carrot tissues, and a positive correlation was observed between the biosynthesis of phenolic compounds and the degradation of sucrose. Simultaneously, wounding activated the sucrose-cleaving enzymes of INVs, including acid INV (AI) and neutral INV (NI), and up-regulated the expressions of most encoding genes of INVs. In addition, treatment with INV activators accelerated the accumulation of phenolic antioxidants, while treatment with INV inhibitors suppressed this process, suggesting that the synthesis of phenolic compounds in wounded carrots is closely related to the availability of sugars. Our findings provide new insights into the regulation role of INV on the wound-induced accumulation of phenolic compounds in carrots, which may be helpful in using wounded plants to produce more phenolic antioxidants.
Ferroptosis, a Regulated Form of Cell Death, as a Target for the Development of Novel Drugs Preventing Ischemia/Reperfusion of Cardiac Injury, Cardiomyopathy and Stress-Induced Cardiac Injury
Authors: Ryabov Vyacheslav V. , Maslov Leonid N. , Vyshlov Evgeniy V. , Mukhomedzyanov Alexander V. , Kilin Mikhail , Gusakova Svetlana V. , Gombozhapova Alexandra E. , Panteleev Oleg O.
Published: January 2024

The hospital mortality in patients with ST-segment elevation myocardial infarction (STEMI) is about 6% and has not decreased in recent years. The leading cause of death of these patients is ischemia/reperfusion (I/R) cardiac injury. It is quite obvious that there is an urgent need to create new drugs for the treatment of STEMI based on knowledge about the pathogenesis of I/R cardiac injury, in particular, based on knowledge about the molecular mechanism of ferroptosis. In this study, it was demonstrated that ferroptosis is involved in the development of I/R cardiac injury, antitumor drug-induced cardiomyopathy, diabetic cardiomyopathy, septic cardiomyopathy, and inflammation. There is indirect evidence that ferroptosis participates in stress-induced cardiac injury. The activation of AMPK, PKC, ERK1/2, PI3K, and Akt prevents myocardial ferroptosis. The inhibition of HO-1 alleviates myocardial ferroptosis. The roles of GSK-3β and NOS in the regulation of ferroptosis require further study. The stimulation of Nrf2, STAT3 prevents ferroptosis. The activation of TLR4 and NF-κB promotes ferroptosis of cardiomyocytes. MiR-450b-5p and miR-210-3p can increase the tolerance of cardiomyocytes to hypoxia/reoxygenation through the inhibition of ferroptosis. Circ_0091761 RNA, miR-214-3p, miR-199a-5p, miR-208a/b, miR-375-3p, miR-26b-5p and miR-15a-5p can aggravate myocardial ferroptosis.
Therapeutic Effect of an Ursolic Acid-Based Nutraceutical on Neuronal Regeneration after Sciatic Nerve Injury
Authors: Iannuzzo Fortuna , Cicatiello Annunziata Gaetana , Sagliocchi Serena , Schiano Elisabetta , Nappi Annarita , Miro Caterina , Stornaiuolo Mariano , Mollica Adriano , Tenore Gian Carlo , Dentice Monica , Novellino Ettore
Published: January 2024

Peripheral nerve injuries lead to severe functional impairments and long recovery times, with limited effectiveness and accessibility of current treatments. This has increased interest in natural bioactive compounds, such as ursolic acid (UA). Our study evaluated the effect of an oleolyte rich in UA from white grape pomace (WGPO) on neuronal regeneration in mice with induced sciatic nerve resection, administered concurrently with the induced damage (the WGPO group) and 10 days prior (the PRE-WGPO group). The experiment was monitored at two-time points (4 and 10 days) after injury. After 10 days, the WGPO group demonstrated a reduction in muscle atrophy, evidenced by an increased number and diameter of muscle fibers and a decreased Atrogin-1 and Murf-1 expression relative to the denervated control. It was also observed that 85.7% of neuromuscular junctions (NMJs) were fully innervated, as indicated by the colocalization of α-bungarotoxin and synaptophysin, along with the significant modulation of Oct-6 and S-100. The PRE-WGPO group showed a more beneficial effect on nerve fiber reformation, with a significant increase in myelin protein zero and 95.2% fully innervated NMJs, and a pro-hypertrophic effect in resting non-denervated muscles. Our findings suggest WGPO as a potential treatment for various conditions that require the repair of nerve and muscle injuries.
Glaucoma Animal Models beyond Chronic IOP Increase
Authors: Tsai Teresa , Reinehr Sabrina , Deppe Leonie , Strubbe Alexandra , Kluge Nils , Dick H. Burkhard , Joachim Stephanie C.
Published: January 2024

Glaucoma is a complex and multifactorial disease defined as the loss of retinal ganglion cells (RGCs) and their axons. Besides an elevated intraocular pressure (IOP), other mechanisms play a pivotal role in glaucoma onset and progression. For example, it is known that excitotoxicity, immunological alterations, ischemia, and oxidative stress contribute to the neurodegeneration in glaucoma disease. To study these effects and to discover novel therapeutic approaches, appropriate animal models are needed. In this review, we focus on various glaucoma animal models beyond an elevated IOP. We introduce genetically modified mice, e.g., the optineurin E50K knock-in or the glutamate aspartate transporter (GLAST)-deficient mouse. Excitotoxicity can be mimicked by injecting the glutamate analogue N-methyl-D-aspartate intravitreally, which leads to rapid RGC degeneration. To explore the contribution of the immune system, the experimental autoimmune glaucoma model can serve as a useful tool. Here, immunization with antigens led to glaucoma-like damage. The ischemic mechanism can be mimicked by inducing a high IOP for a certain amount of time in rodents, followed by reperfusion. Thereby, damage to the retina and the optic nerve occurs rapidly after ischemia/reperfusion. Lastly, we discuss the importance of optic nerve crush models as model systems for normal-tension glaucoma. In summary, various glaucoma models beyond IOP increase can be utilized.
Single-Cell Transcriptome Analysis Reveals Development-Specific Networks at Distinct Synchronized Antral Follicle Sizes in Sheep Oocytes
Authors: Song Yukun , Zhang Nan , Zhang Yu , Wang Junlan , Lv Qi , Zhang Jiaxin
Published: January 2024

The development of the ovarian antral follicle is a complex, highly regulated process. Oocytes orchestrate and coordinate the development of mammalian ovarian follicles, and the rate of follicular development is governed by a developmental program intrinsic to the oocyte. Characterizing oocyte signatures during this dynamic process is critical for understanding oocyte maturation and follicular development. Although the transcriptional signature of sheep oocytes matured in vitro and preovulatory oocytes have been previously described, the transcriptional changes of oocytes in antral follicles have not. Here, we used single-cell transcriptomics (SmartSeq2) to characterize sheep oocytes from small, medium, and large antral follicles. We characterized the transcriptomic landscape of sheep oocytes during antral follicle development, identifying unique features in the transcriptional atlas, stage-specific molecular signatures, oocyte-secreted factors, and transcription factor networks. Notably, we identified the specific expression of 222 genes in the LO, 8 and 6 genes that were stage-specific in the MO and SO, respectively. We also elucidated signaling pathways in each antral follicle size that may reflect oocyte quality and in vitro maturation competency. Additionally, we discovered key biological processes that drive the transition from small to large antral follicles, revealing hub genes involved in follicle recruitment and selection. Thus, our work provides a comprehensive characterization of the single-oocyte transcriptome, filling a gap in the mapping of the molecular landscape of sheep oogenesis. We also provide key insights into the transcriptional regulation of the critical sizes of antral follicular development, which is essential for understanding how the oocyte orchestrates follicular development.
Molecular and Systems Biology Approaches for Harnessing the Symbiotic Interaction in Mycorrhizal Symbiosis for Grain and Oil Crop Cultivation
Authors: Slimani Aiman , Ait-El-Mokhtar Mohamed , Ben-Laouane Raja , Boutasknit Abderrahim , Anli Mohamed , Abouraicha El Faiza , Oufdou Khalid , Meddich Abdelilah , Baslam Marouane
Published: January 2024

Mycorrhizal symbiosis, the mutually beneficial association between plants and fungi, has gained significant attention in recent years due to its widespread significance in agricultural productivity. Specifically, arbuscular mycorrhizal fungi (AMF) provide a range of benefits to grain and oil crops, including improved nutrient uptake, growth, and resistance to (a)biotic stressors. Harnessing this symbiotic interaction using molecular and systems biology approaches presents promising opportunities for sustainable and economically-viable agricultural practices. Research in this area aims to identify and manipulate specific genes and pathways involved in the symbiotic interaction, leading to improved cereal and oilseed crop yields and nutrient acquisition. This review provides an overview of the research frontier on utilizing molecular and systems biology approaches for harnessing the symbiotic interaction in mycorrhizal symbiosis for grain and oil crop cultivation. Moreover, we address the mechanistic insights and molecular determinants underpinning this exchange. We conclude with an overview of current efforts to harness mycorrhizal diversity to improve cereal and oilseed health through systems biology.
A Water-Soluble Chitosan Derivative for the Release of Bioactive Deferoxamine
Authors: Michailidou Georgia , Li Yupeng , Zamboulis Alexandra , Karlioti Georgia , Meimaroglou Despoina , Pantopoulos Kostas , Bikiaris Dimitrios N.
Published: January 2024

Deferoxamine (DFO) is a water-soluble iron chelator used pharmacologically for the management of patients with transfusional iron overload. However, DFO is not cell-permeable and has a short plasma half-life, which necessitates lengthy parenteral administration with an infusion pump. We previously reported the synthesis of chitosan (CS) nanoparticles for sustained slow release of DFO. In the present study, we developed solid dispersions and nanoparticles of a carboxymethyl water-soluble chitosan derivative (CMCS) for improved DFO encapsulation and release. CS dispersions and nanoparticles with DFO have been prepared by ironical gelation using sodium triphosphate (TPP) and were examined for comparison purposes. The successful presence of DFO in CMCS polymeric dispersions and nanoparticles was confirmed through FTIR measurements. Furthermore, the formation of CMCS nanoparticles led to inclusion of DFO in an amorphous state, while dispersion of DFO in the polymeric matrix led to a decrease in its crystallinity according to X-ray diffraction (XRD) and differential scanning calorimetry (DSC) results. An in vitro release assay indicated sustained release of DFO from CS and CMCS nanoparticles over 48 h and 24 h, respectively. Application of CMCS-DFO dispersions to murine RAW 264.7 macrophages or human HeLa cervical carcinoma cells triggered cellular responses to iron deficiency. These were exemplified in the induction of the mRNA encoding transferrin receptor 1, the major iron uptake protein, and the suppression of ferritin, the iron storage protein. Our data indicate that CMCS-DFO nanoparticles release bioactive DFO that causes effective iron chelation in cultured cells.
Posttranslational Acylations of the Rat Brain Transketolase Discriminate the Enzyme Responses to Inhibitors of ThDP-Dependent Enzymes or Thiamine Transport
Authors: Aleshin Vasily A. , Kaehne Thilo , Maslova Maria V. , Graf Anastasia V. , Bunik Victoria I.
Published: January 2024

Transketolase (TKT) is an essential thiamine diphosphate (ThDP)-dependent enzyme of the non-oxidative branch of the pentose phosphate pathway, with the glucose-6P flux through the pathway regulated in various medically important conditions. Here, we characterize the brain TKT regulation by acylation in rats with perturbed thiamine-dependent metabolism, known to occur in neurodegenerative diseases. The perturbations are modeled by the administration of oxythiamine inhibiting ThDP-dependent enzymes in vivo or by reduced thiamine availability in the presence of metformin and amprolium, inhibiting intracellular thiamine transporters. Compared to control rats, chronic administration of oxythiamine does not significantly change the modification level of the two detected TKT acetylation sites (K6 and K102) but doubles malonylation of TKT K499, concomitantly decreasing 1.7-fold the level of demalonylase sirtuin 5. The inhibitors of thiamine transporters do not change average levels of TKT acylation or sirtuin 5. TKT structures indicate that the acylated residues are distant from the active sites. The acylations-perturbed electrostatic interactions may be involved in conformational shifts and/or the formation of TKT complexes with other proteins or nucleic acids. Acetylation of K102 may affect the active site entrance/exit and subunit interactions. Correlation analysis reveals that the action of oxythiamine is characterized by significant negative correlations of K499 malonylation or K6 acetylation with TKT activity, not observed upon the action of the inhibitors of thiamine transport. However, the transport inhibitors induce significant negative correlations between the TKT activity and K102 acetylation or TKT expression, absent in the oxythiamine group. Thus, perturbations in the ThDP-dependent catalysis or thiamine transport manifest in the insult-specific patterns of the brain TKT malonylation and acetylations.
Laboratory Liquid-Jet X-ray Microscopy and X-ray Fluorescence Imaging for Biomedical Applications
Authors: Arsana Komang G. Y. , Saladino Giovanni M. , Brodin Bertha , Toprak Muhammet S. , Hertz Hans M.
Published: January 2024

Diffraction-limited resolution and low penetration depth are fundamental constraints in optical microscopy and in vivo imaging. Recently, liquid-jet X-ray technology has enabled the generation of X-rays with high-power intensities in laboratory settings. By allowing the observation of cellular processes in their natural state, liquid-jet soft X-ray microscopy (SXM) can provide morphological information on living cells without staining. Furthermore, X-ray fluorescence imaging (XFI) permits the tracking of contrast agents in vivo with high elemental specificity, going beyond attenuation contrast. In this study, we established a methodology to investigate nanoparticle (NP) interactions in vitro and in vivo, solely based on X-ray imaging. We employed soft (0.5 keV) and hard (24 keV) X-rays for cellular studies and preclinical evaluations, respectively. Our results demonstrated the possibility of localizing NPs in the intracellular environment via SXM and evaluating their biodistribution with in vivo multiplexed XFI. We envisage that laboratory liquid-jet X-ray technology will significantly contribute to advancing our understanding of biological systems in the field of nanomedical research.
Expression Pattern and Prognostic Significance of the Long Non-Coding RNA Metastasis-Associated Lung Adenocarcinoma Transcript 1 in Chronic Lymphocytic Leukemia
Authors: Tomic Vujovic Kristina , Ugrin Milena , Tosic Natasa , Vukovic Vojin , Marjanovic Irena , Kostic Tatjana , Stankovic Sanja , Otasevic Vladimir , Sarac Sofija , Antic Darko , Pavlovic Sonja , Karan-Djurasevic Teodora
Published: January 2024

Dysregulated expression of the long non-coding RNA MALAT1 has been implicated in the pathogenesis and progression of a variety of cancers, including hematological malignancies, but it has been poorly investigated in chronic lymphocytic leukemia (CLL). In this study, the expression of MALAT1 was measured using a quantitative reverse-transcriptase polymerase chain reaction in the peripheral blood mononuclear cells of 114 unselected, newly diagnosed CLL patients in order to analyze its association with clinical, laboratory, and molecular patients’ characteristics at diagnosis, as well as its prognostic relevance. MALAT1 was found to be upregulated in CLL patients in comparison to healthy controls, and expression levels were not related to age, leukocyte, lymphocyte and platelet count, serum β2-microglobulin, and IGHV somatic hypermutational status. On the other hand, high MALAT1 expression was associated with several favorable prognostic markers (high hemoglobin, low serum lactate dehydrogenase, earlier clinical stages, CD38-negative status), but also with unfavorable cytogenetics. Furthermore, an association between high MALAT1 levels and longer time to first treatment and overall survival in IGHV-unmutated CLL subtype was observed. In summary, our results imply that high MALAT1 expression at diagnosis may be a predictor of better prognosis and point to MALAT1 expression profiling as a candidate biomarker potentially useful in clinical practice.
Fibrous Remodeling in Eosinophilic Esophagitis: Clinical Facts and Pathophysiological Uncertainties
Authors: Arias-González Laura , Rodríguez-Alcolado Leticia , Laserna-Mendieta Emilio J. , Navarro Pilar , Lucendo Alfredo J. , Grueso-Navarro Elena
Published: January 2024

Eosinophilic esophagitis (EoE) is a chronic, progressive, type 2 inflammatory disease with increasing global prevalence. An eosinophil-predominant inflammation that permeates the epithelium and deeper esophageal layers characterizes the disease. Several cytokines, mainly derived from inflammatory T-helper 2 (Th2) cells and epithelial cells, are involved in perpetuating inflammatory responses by increasing surface permeability and promoting tissue remodeling characterized by epithelial–mesenchymal transition (EMT) and collagen deposition. This leads to esophageal strictures and narrow caliber esophagi, which are proportional a patient’s age and untreated disease length. Pathophysiological mechanisms leading to EoE have been described in recent years, and transforming growth factor beta (TGF)-beta have been involved in fibrotic phenomena in EoE. However, evidence on the dependence of these phenomena on TGF-beta is scarce and contradictory. This review provides state-of-the art knowledge on intimate mechanisms of esophageal fibrosis in EoE and its clinical consequences.
Transcriptomic Evidence of a Link between Cell Wall Biogenesis, Pathogenesis, and Vigor in Walnut Root and Trunk Diseases
Authors: Saxe Houston J. , Walawage Sriema L. , Balan Bipin , Leslie Charles A. , Brown Patrick J. , Browne Gregory T. , Kluepfel Daniel A. , Westphal Andreas , Dandekar Abhaya M.
Published: January 2024

Crown gall disease (Agrobacterium tumefaciens), crown/root rot disease (Phytophthora spp.), root lesion disease (Pratylenchus vulnus) and tree vigor are key traits affecting the productivity and quality of walnuts in California. Unchallenged hybrid rootstocks were analyzed by RNA-seq to examine pre-formed factors affecting these traits. Enrichment analysis of the differentially expressed genes revealed that the increased expression of cell wall biogenesis-related genes plays a key role in susceptibility to A. tumefaciens, susceptibility to Phytophthora spp. and increased vigor. Analysis of the predicted subcellular loci of the encoded proteins revealed that many gene products associated with vigor and susceptibility were targeted to the plasma membrane and extracellular space, connecting these traits to sustaining barrier function. We observed that RNA processing and splicing, along with predicted nuclear targeting, were associated with resistance to A. tumefaciens, resistance to Phytophthora spp. and low vigor. Four genes within the J. microcarpa QTL region for resistance to A. tumefaciens and Phytophthora spp. were represented among our transcripts, with two of the genes being differentially expressed in association with resistance to A. tumefaciens and decreased vigor. No differential expression related to Phytophthora spp. or P. vulnus resistance was observed in this region. Additionally, the J. microcarpa haplotype expressed more transcripts associated with resistance to A. tumefaciens, Phytophthora spp. and low vigor, but not P. vulnus, than the J. regia haplotype. We also report unique and shared hormone and defense responses associated with each trait. This research suggests a link between cell wall biogenesis, vigor and critical root diseases of walnut.
Inconsistencies in the Classification of the Family Cydnidae (Hemiptera: Heteroptera: Pentatomoidea) Revealed by Molecular Apomorphies in the Secondary and Tertiary Structures of 18S rRNA Length-Variable Region L (LVR L)
Authors: Lis Jerzy A. , Domagała Paweł J.
Published: January 2024

The SSU nuclear rDNA (encoding 18S ribosomal RNA) is one of the most frequently sequenced genes in the molecular analysis of insects. Molecular apomorphies in the secondary and tertiary structures of several 18S rRNA length-variable regions (LVRs) located within the V2, V4, and V7 hypervariable regions can be good indicators for recovering monophyletic groups within some heteropteran families. Among the LVRs that have been analysed, the LVR L in the V4 hypervariable region is the longest and most crucial for such assessments. We analysed the 18S rRNA V4 hypervariable region sequences of 45 species from the family Cydnidae, including all 6 subfamilies (Amaurocorinae, Amnestinae, Cephalocteinae, Cydninae, Garsauriinae, and Sehirinae) and three pentatomoid families (Parastrachiidae, Thaumastellidae, and Thyreocoridae), which have often been included in the broadly defined Cydnidae family. This is the first time that representatives of all Cydnidae subfamilies have been included in a molecular analysis. Only taxa from two subfamilies, Sehirinae and Cydninae, have been used in previous molecular studies. The secondary and tertiary structures of the LVR L were predicted for each species using the two-step procedure already accepted for such analyses to recover any molecular apomorphy essential for determining monophyly. The results of our comparative studies contradict the current understanding of the relationships among burrowing bugs and the current family classification.
Oregano (Origanum vulgare) Essential Oil and Its Constituents Prevent Rat Kidney Tissue Injury and Inflammation Induced by a High Dose of L-Arginine
Authors: Stojanović Nikola M. , Mitić Katarina V. , Nešić Milica , Stanković Milica , Petrović Vladimir , Baralić Marko , Randjelović Pavle J. , Sokolović Dušan , Radulović Niko
Published: January 2024

This study aimed to evaluate the protective action of oregano (Origanum vulgare) essential oil and its monoterpene constituents (thymol and carvacrol) in L-arginine-induced kidney damage by studying inflammatory and tissue damage parameters. The determination of biochemical markers that reflect kidney function, i.e., serum levels of urea and creatinine, tissue levels of neutrophil-gelatinase-associated lipocalin (NGAL), and kidney injury molecule-1 (KIM-1), as well as a panel of oxidative-stress-related and inflammatory biomarkers, was performed. Furthermore, histopathological and immunohistochemical analyses of kidneys obtained from different experimental groups were conducted. Pre-treatment with the investigated compounds prevented an L-arginine-induced increase in serum and tissue kidney damage markers and, additionally, decreased the levels of inflammation-related parameters (TNF-α and nitric oxide concentrations and myeloperoxidase activity). Micromorphological kidney tissue changes correlate with the alterations observed in the biochemical parameters, as well as the expression of CD95 in tubule cells and CD68 in inflammatory infiltrate cells. The present results revealed that oregano essential oil, thymol, and carvacrol exert nephroprotective activity, which could be, to a great extent, associated with their anti-inflammatory, antiradical scavenging, and antiapoptotic action and, above all, due to their ability to lessen the disturbances arising from acute pancreatic damage. Further in-depth studies are needed in order to provide more detailed explanations of the observed activities.
Effects of Aminomethylphosphonic Acid on the Transcriptome and Metabolome of Red Swamp Crayfish, Procambarus clarkii
Authors: Mao Tao , Gan Jinhua , Yuan Keping , He Li , Yu Yali , Liu Ziduo , Zhou Yuntao , Wu Gaobing
Published: January 2024

Red swamp crayfish, Procambarus clarkii (P. clarkii), is an important model crustacean organism used in many types of research. However, the effects of different doses of aminomethylphosphonic acid (AMAP) on the transcriptome and metabolites of P. clarkii have not been explored. Thus, this study investigated the molecular and metabolic mechanisms activated at the different exposure dosages of AMAP in P. clarkii to provide new insights into the strategies of P. clarkii in response to the high concentrations of AMAP in the environment. In the present study, the P. clarkii were divided into three groups (control group; low-dosage AMAP exposure; high-dosage AMAP exposure), and hepatopancreatic tissue samples were dependently taken from the three groups. The response mechanisms at the different dosages of AMAP were investigated based on the transcriptome and metabolome data of P. clarkii. Differentially expressed genes and differentially abundant metabolites were identified in the distinct AMAP dosage exposure groups. The genes related to ribosome cell components were significantly up-regulated, suggesting that ribosomes play an essential role in responding to AMAP stress. The metabolite taurine, involved in the taurine and hypotaurine metabolism pathway, was significantly down-regulated. P. clarkii may provide feedback to counteract different dosages of AMAP via the upregulation of ribosome-related genes and multiple metabolic pathways. These key genes and metabolites play an important role in the response to AMAP stress to better prepare for survival in high AMAP concentrations.
From SuperTIGER to TIGERISS
Authors: Rauch B. F. , Zober W. V. , Abarr Q. , Akaike Y. , Binns W. R. , Borda R. F. , Bose R. G. , Brandt T. J. , Braun D. L. , Buckley J. H. , Cannady N. W. , Coutu S. , Crabill R. M. , Dowkontt P. F. , Israel M. H. , Kandula M. , Krizmanic J. F. , Labrador A. W. , Labrador W. , Lisalda L. , Martins J. V. , McPherson M. P. , Mewaldt R. A. , Mitchell J. G. , Mitchell J. W. , Mognet S. A. I. , Murphy R. P. , de Nolfo G. A. , Nutter S. , Olevitch M. A. , Osborn N. E. , Pastrana I. M. , Sakai K. , Sasaki M. , Smith S. , Tolentino H. A. , Walsh N. E. , Ward J. E. , Washington D. , West A. T. , Williams L. P.
Published: January 2024

The Trans-Iron Galactic Element Recorder (TIGER) family of instruments is optimized to measure the relative abundances of the rare, ultra-heavy galactic cosmic rays (UHGCRs) with atomic number (Z) Z ≥ 30. Observing the UHGCRs places a premium on exposure that the balloon-borne SuperTIGER achieved with a large area detector (5.6 m2) and two Antarctic flights totaling 87 days, while the smaller (∼1 m2) TIGER for the International Space Station (TIGERISS) aims to achieve this with a longer observation time from one to several years. SuperTIGER uses a combination of scintillator and Cherenkov detectors to determine charge and energy. TIGERISS will use silicon strip detectors (SSDs) instead of scintillators, with improved charge resolution, signal linearity, and dynamic range. Extended single-element resolution UHGCR measurements through 82Pb will cover elements produced in s-process and r-process neutron capture nucleosynthesis, adding to the multi-messenger effort to determine the relative contributions of supernovae (SNe) and Neutron Star Merger (NSM) events to the r-process nucleosynthesis product content of the galaxy.
Exploring QRS Area beyond Patient Selection in CRT—Can It Guide Left Ventricular Lead Placement?
Authors: Eerenberg Frederieke , Luermans Justin , Lumens Joost , Nguyên Uyên Châu , Vernooy Kevin , van Stipdonk Antonius
Published: January 2024

Vectorcardiographic QRS area is a promising tool for patient selection and implantation guidance in cardiac resynchronization therapy (CRT). Research has mainly focused on the role of QRS area in patient selection for CRT. Recently, QRS area has been proposed as a tool to guide left ventricular lead placement in CRT. Theoretically, vector-based electrical information of ventricular fusion pacing, calculated from the basic 12-lead ECG, can give real-time insight into the extent of resynchronization at any LV lead position, as well as any selected electrode on the LV lead. The objective of this review is to provide an overview of the background of vectorcardiographic QRS area and its potential in optimizing LV lead location in order to optimize the benefits of CRT.
Kaolin–Polyvinyl Alcohol–Potato Starch Composite Films for Environmentally Friendly Packaging: Optimization and Characterization
Authors: Tabassum Noshabah , Rafique Uzaira , Qayyum Maria , Mohammed Abdallah A. A. , Asif Saira , Bokhari Awais
Published: January 2024

This research paper introduces an innovative methodology to produce biodegradable composite films by combining kaolin, polyvinyl alcohol (PVA), and potato starch (PS) using a solvent casting technique. The novelty of this study resides in the identification and implementation of optimal synthesis conditions, which were achieved by utilizing the Response Surface Methodology—Central Composite Design. The study defines starch, polyvinyl alcohol (PVA), and kaolin as independent variables and examines their influence on important mechanical qualities, water absorption capacity, moisture content, and degradability as primary outcomes. The study establishes the ideal parameters as 5.5 weight percent Kaolin, 2.5 g of starch, and 3.5 g of PVA. These settings yield notable outcomes, including a tensile strength of 26.5 MPa, an elongation at break of 96%, a water absorption capacity of 21%, a moisture content of 3%, and a remarkable degradability of 48%. The study emphasizes that the augmentation of kaolin content has a substantial impact on many properties, including degradability, tensile strength, and elongation at break. Simultaneously, it leads to a reduction in the water absorption capacity and moisture content. The study’s novelty is reinforced by conducting an additional examination on the ideal composite film, which includes investigations using FTIR, TGA, and SEM-EDX techniques. The consistency between the predicted and experimental results is noteworthy, as it provides further validation for the prediction accuracy of Design Expert software’s quadratic equations. These equations effectively capture the complex interactions that exist between process parameters and selected responses. This study presents novel opportunities for the extensive utilization of PVA/PS composite films, including kaolin in various packaging scenarios, thereby significantly advancing sustainable packaging alternatives. The statistical analysis provides strong evidence supporting the relevance of the models, hence increasing our level of trust in the software’s prediction skills. This conclusion is based on a 95% confidence level and p-values that are below a threshold of 0.05.
Effects of Self-Myofascial Release on Athletes’ Physical Performance: A Systematic Review
Authors: Martínez-Aranda Luis Manuel , Sanz-Matesanz Manuel , García-Mantilla Ezequiel David , González-Fernández Francisco Tomás
Published: January 2024

Therapists and strength and conditioning specialists use self-myofascial release (SMR) as an intervention tool through foam rollers or massage rollers for soft tissue massage, with the purpose of improving mobility in the muscular fascia. Moreover, the use of SMR by professional and amateur athletes during warm-ups, cool downs, and workouts can have significant effects on their physical performance attributes, such as range of motion (ROM) and strength. The purpose of this study was to analyse the literature pertaining to these types of interventions and their effects found in different physical performance attributes for athletes. A systematic search was carried out using the following databases: PUBMED, ISI Web of Science, ScienceDirect, and Cochrane, including articles up to September 2023. A total of 25 articles with 517 athletes were studied in depth. SMR seems to have acute positive effects on flexibility and range of motion, without affecting muscle performance during maximal strength and power actions, but favouring recovery perception and decreasing delayed-onset muscle soreness. Some positive effects on agility and very short-range high-speed actions were identified, as well. In conclusion, although there is little evidence of its method of application due to the heterogeneity in that regard, according to our findings, SMR could be used as an intervention to improve athletes’ perceptual recovery parameters, in addition to flexibility and range of motion, without negatively affecting muscle performance.
Russia–Ukraine Conflict, Commodities and Stock Market: A Quantile VAR Analysis
Authors: Manelli Alberto , Pace Roberta , Leone Maria
Published: January 2024

The Russia–Ukrainian war, which began in 2014 and exploded with the invasion of the Russian army on 24 February 2022, has profoundly destabilized the political, economic and financial balance of Europe and beyond. To the humanitarian emergency associated with every war has been added the deep crisis generated by the strong energy and food dependence that many European countries, and not only European, have developed over decades on Ukraine (especially for wheat) and Russia (especially for natural gas). The aim of this article is to verify the existence of a link between the performance of the Eurostoxx index and the price of wheat futures and TTF natural gas, from 25 February 2019 to 28 September 2023. Through a quantile VAR analysis, a link is sought between the Eurostoxx 50 index, and wheat and TTF gas futures prices. Furthermore, the analysis intends to understand whether the presence of such relationship only manifested itself following the war events, or whether it was already present in the market. The analysis carried out also shows that the relationship between the stock market and raw material prices was present even before the conflict.
A Comparative Multi-Zoo Survey Investigating the Housing and Husbandry of Callimico goeldii
Authors: Bartlett Amanda , Brereton James Edward , Freeman Marianne Sarah
Published: January 2024

Callimico (Callimico goeldii) are members of the callitrichid family, and a species of conservation concern managed within the European Association of Zoos and Aquaria (EAZA) ex situ program. They benefit from extensive ‘Best Practice’ guidelines developed by the Callitrichid Taxon Advisory Group (TAG), but there are gaps in species-specific knowledge and captive management research. A survey was created to understand and evaluate current housing and husbandry within EAZA collections holding callimico. The questionnaire allowed collation of details including enclosure size along with prevalence of mixed species exhibits, use of UV-B lighting, enclosure complexity and enrichment routines. Responding collections represented 44% of the current callimico holders, with the results allowing comparative analysis of current practice against the guidelines and considering previous research. Significant positive differences were discovered between minimum recommended enclosure dimensions, including total enclosure volume, 32 m3 (p < 0.001, median = 100) and height, 2.5 m (p < 0.001, median = 3) versus EAZA’s minimum recommended dimensions. Encouragingly, no significant difference in complexity was found between on and off exhibit housing, but the results suggest that being housed in a mixed species exhibit offers a more complex environment for callimico (W = 405.5, p < 0.005). The responses revealed overall good practice, although a disparity was noted in the provision of UV-B lighting and the prevalence of enrichment. Observational research focusing on spatial use, preference and behavior is recommended to complement husbandry guidelines. Reference of the survey findings to welfare indicators could determine the effect of current housing and husbandry on callimico welfare.
Determining Compressed Concrete Element Limit States Based on the Widths and Depths of Cracks Caused by Transverse Deformations
Authors: Iskhakov Iakov , Frolov Ilya , Ribakov Yuri
Published: January 2024

In the modern theory of compressed concrete elements, the most attention is paid to longitudinal deformations, whereas transverse ones are rarely considered and just within Poisson’s coefficient limits (i.e., elastic concrete behavior in the transverse direction). However, transverse deformations significantly develop beyond the limits corresponding to Poisson’s coefficient, where they lead to longitudinal crack initiation and development. In-depth experimental and numerical investigations of transverse deformations in the inelastic stage showed that it is necessary to consider crack propagation. The present study proposes simultaneous consideration of longitudinal and transverse deformations, as well as the appearance of cracks and their widths and depths. This allowed us to obtain a complete compressed concrete element behavior pattern at all performance stages in two types of limit states (based on longitudinal and transverse deformations). Consequently, new ultimate limit states by the depth and width of cracks caused by transverse deformations are proposed to be included in modern design practices and codes.
Effect of Cold Deformation and Heat Treatment on the Microstructures and Mechanical Properties of Au-15Ag-12Cu-6Ni Alloy Sheets
Authors: Chen Haodong , Cui Xinyue , Hui Songxiao , Li Changheng , Ye Wenjun , Yu Yang
Published: January 2024

The evolution of the microstructure and hardness changes in the Au-15Ag-12Cu-6Ni alloy during the processes of cold rolling and annealing were investigated and the heat treatment regimen for the alloy was optimized in this article. The hardness of the alloy continuously increases with the cold rolling reductions, leading to continuous deformation of the grains during the cold rolling process, ultimately resulting in smaller grain sizes. Subsequent annealing induces recovery and recrystallization, achieving complete recrystallization at 700 °C. An intriguing softening effect is observed after annealing at 700 °C, manifesting in a significant reduction in hardness to 238 (Hv0.5). The cold deformation texture of the alloy aligns with the recrystallization texture type, exhibiting only a certain degree of angular deviation. This is primarily characterized by <111>//RD texture and a texture deviating 60° from RD towards TD. The performance of the finished sheet improves with the precipitation of ordered phases AuCu after a 300 °C heat treatment for 0.5 h, resulting in a remarkable hardness of 380 (Hv0.5).
Petrophysical Analyses of Rock Construction Materials from a Roman Rural Settlement in Podšilo Bay on Rab Island (North-East Adriatic, Croatia)
Authors: Trzciński Jerzy , Wójcik Emilia , Kiełbasiński Kamil , Łukaszewski Paweł , Zaremba Małgorzata , Kaczmarek Łukasz , Dziedziczak Robert , Kotowski Jakub , Konestra Ana , Welc Fabian , Wejrzanowski Tomasz , Jaroszewicz Jakub
Published: January 2024

This article presents the results of petrophysical analyses of limestones and sandstones used for the construction of the wall structures of a Roman rural settlement located in Podšilo Bay on Rab Island (Croatia). An on-site analysis of the walls indicated the use of different lithotypes, which is an uncommon case in the area. So far, no petrophysical properties of the applied materials have been tested, and their provenance has not been specified. The aim of this research was to determine their usability as construction materials in an attempt to determine the possible reasons behind the usage of multiple lithotypes and their suitability as building materials. The following procedure was used to address these issues: (1) determination of the petrographic characteristics of the rocks, (2) performance of tests to characterise the mechanical properties in a complex stress state of uniaxial tension followed by uniaxial and triaxial compression, and, finally, (3) determination of the internal structure of the rocks using methods based on X-ray imaging. Multi-proxy characteristics of the materials including numerous observations and methods were performed: optical microscopy used to characterise rock petrography and mineralogy, scanning electron microscopy (SEM) coupled with EDS, as well as grinding tests; furthermore, mechanical properties were determined on cylindrical samples in accordance with European standards. X-ray microtomography using the XμCT method enabled microscopic observations and determination of the orientation of discontinuities and the rock structure. The performed analyses allowed us to distinguish three lithological types of sandstone and two types of limestone among the examined stone blocks. Stone blocks of fine- and medium-grained sandstone with carbonate binders, as well as sparitic limestone and mudstone with calcite veins, were used to build the studied structures. The analysed blocks showed traces of partial edge processing. Despite the defects in the material structure identified using XμCT, all the types of rock were characterised by high or very high strength. High values of longitudinal wave velocity confirmed the good quality of the material. These results contribute to a better understanding of the construction process and the related technological choices, and they provide the first dataset which can be used for the reconstruction of the building’s original appearance in the future.
Synthesis of Metallic Nanostructures Using Ionizing Radiation and Their Applications
Authors: Remita Hynd , Lampre Isabelle
Published: January 2024

This paper reviews the radiation-induced synthesis of metallic nanostructures and their applications. Radiolysis is a powerful method for synthesizing metallic nanoparticles in solution and heterogeneous media, and it is a clean alternative to other existing physical, chemical, and physicochemical methods. By varying parameters such as the absorbed dose, dose rate, concentrations of metallic precursors, and nature of stabilizing agents, it is possible to control the size, shape, and morphology (alloy, core-shell, etc.) of the nanostructures and, consequently, their properties. Therefore, the as-synthesized nanoparticles have many potential applications in biology, medicine, (photo)catalysis, or energy conversion.
The Effect of Restoration Thickness on the Fracture Resistance of 5 mol% Yttria-Containing Zirconia Crowns
Authors: Chen Po-Hsu , Elamin Esra , Sayed Ahmed Akram , Givan Daniel A. , Fu Chin-Chuan , Lawson Nathaniel C.
Published: January 2024

Background: To determine what thickness of 5 mol% yttria zirconia (5Y-Z) translucent crowns cemented with different cements and surface treatments would have equivalent fracture resistance as 3 mol% yttria (3Y-Z) crowns. Methods: The study included 0.8 mm, 1.0 mm, and 1.2 mm thickness 5Y-Z (Katana UTML) crowns and 0.5 and 1.0 mm thickness 3Y-Z (Katana HT) crowns as controls. The 5Y-Z crowns were divided among three treatment subgroups (n = 10/subgroup): (1) cemented using RMGIC (Rely X Luting Cement), (2) alumina particle-abraded then luted with the same cement, (3) alumina particle-abraded and cemented using a resin cement (Panavia SA Cement Universal). The 3Y-Z controls were alumina particle-abraded then cemented with RMGIC. The specimens were then loaded in compression at 30° until failure. Results: All 5Y-Z crowns (regardless of thickness or surface treatment) had a similar to or higher fracture force than the 0.5 mm 3Y-Z crowns. Only the 1.2 mm 5Y-Z crowns with resin cement showed significantly similar fracture force to the 1 mm 3Y-Z crowns. Conclusion: In order to achieve a similar fracture resistance to 0.5 mm 3Y-Z crowns cemented with RMGIC, 5Y-Z crowns may be as thin as 0.8 mm. To achieve a similar fracture resistance to 1.0 mm 3Y-Z crowns cemented with RMGIC, 5Y-Z crowns must be 1.2 mm and bonded with resin cement.
The Two-Domain Model Utilizing the Effective Pinning Energy for Modeling the Strain-Dependent Magnetic Permeability in Anisotropic Grain-Oriented Electrical Steels
Authors: Szumiata Tadeusz , Rekas Paweł , Gzik-Szumiata Małgorzata , Nowicki Michał , Szewczyk Roman
Published: January 2024

This paper presents a newly proposed domain wall energy-based model of the 2D strain dependence of relative magnetic permeability in highly grain-oriented anisotropic electrical steels. The model was verified utilizing grain-oriented M120-27s electrical steel sheet samples with magnetic characteristics measured by an automated experimental setup with a magnetic yoke. The model’s parameters, identified in the differential evolution-based optimization process, enable a better understanding of the interaction between stress-induced anisotropy and magnetocrystalline anisotropy in electrical steels. Moreover, the consequences of the simplified description of grain-oriented magnetocrystalline anisotropy are clearly visible, which opens up the possibility for further research to improve this description.
Temperature Field Characterization of Iron Tailings Based on Microwave Maintenance Technology
Authors: Xue Jun , Liu Shengjie , Xu Meng , Ling Meng , Sun Jinbao , Li Hui , Kang Xianzhang
Published: January 2024

Microwave maintenance technology, as a new development trend, can realize the environmentally noninvasive and rapid repair of asphalt pavement and gradually replace traditional maintenance methods. Iron tailings were used as a self-healing material in this study to investigate the temperature response matching of microwave maintenance technology. Firstly, the physical properties and the mechanism of iron tailings were elaborated through macroscopic physical index testing and microscopic X-ray diffraction (XRD) analysis. Secondly, the applicability of aggregates to microwave heating was demonstrated by analyzing the temperature rise characteristics of the granules using infrared imaging. Then, the temperature field variation rules of the iron tailing asphalt mixture were summarized by microwave heating Marshall specimens. Finally, the road performance was assessed by conducting high-temperature dynamic stability, low-temperature tensile, water immersion Marshall, and freeze-thaw splitting tests. The experimental results showed that the iron tailings can be used as an aggregate for high-grade asphalt pavement and as the preferred aggregate for microwave maintenance technology. The iron tailings temperature field was radial from the inside out to provide different temperature response states for different pavement diseases, so the asphalt was dissolved and precipitated in a short time. The particle size of iron tailings was inversely proportional to the wave-absorbing heating rate, and the heating efficiency of the small particle size (0–4.75 mm) was the highest. The specimens doped with 4.75–13.2 mm iron tailings showed the best heating performance and road performance, with the average surface temperature of the specimens reaching 126.0 °C within 2 min. In summary, according to different disease types and construction needs, iron tailings can be used as an aggregate for asphalt pavement, providing an appropriate temperature field and improving the efficiency of the microwave maintenance of asphalt pavements.