bluuebunny/crossref_metadata_2025 huggingface dataset:
Rice Stomatal Mega-Papillae Restrict Water Loss and Pathogen Entry
Authors: Pitaloka Mutiara K. , Harrison Emily L. , Hepworth Christopher , Wanchana Samart , Toojinda Theerayut , Phetluan Watchara , Brench Robert A. , Narawatthana Supatthra , Vanavichit Apichart , Gray Julie E. , Caine Robert S. , Arikit Siwaret
Published: June 2021

Rice (Oryza sativa) is a water-intensive crop, and like other plants uses stomata to balance CO2 uptake with water-loss. To identify agronomic traits related to rice stomatal complexes, an anatomical screen of 64 Thai and 100 global rice cultivars was undertaken. Epidermal outgrowths called papillae were identified on the stomatal subsidiary cells of all cultivars. These were also detected on eight other species of the Oryza genus but not on the stomata of any other plant species we surveyed. Our rice screen identified two cultivars that had “mega-papillae” that were so large or abundant that their stomatal pores were partially occluded; Kalubala Vee had extra-large papillae, and Dharia had approximately twice the normal number of papillae. These were most accentuated on the flag leaves, but mega-papillae were also detectable on earlier forming leaves. Energy dispersive X-Ray spectrometry revealed that silicon is the major component of stomatal papillae. We studied the potential function(s) of mega-papillae by assessing gas exchange and pathogen infection rates. Under saturating light conditions, mega-papillae bearing cultivars had reduced stomatal conductance and their stomata were slower to close and re-open, but photosynthetic assimilation was not significantly affected. Assessment of an F3 hybrid population treated with Xanthomonas oryzae pv. oryzicola indicated that subsidiary cell mega-papillae may aid in preventing bacterial leaf streak infection. Our results highlight stomatal mega-papillae as a novel rice trait that influences gas exchange, stomatal dynamics, and defense against stomatal pathogens which we propose could benefit the performance of future rice crops.
Larvicidal Activity of Essential Oils From Piper Species Against Strains of Aedes aegypti (Diptera: Culicidae) Resistant to Pyrethroids
Authors: Pereira Filho Adalberto Alves , Pessoa Grasielle C. D‘Ávila , Yamaguchi Lydia F. , Stanton Mariana Alves , Serravite Artur M. , Pereira Rafael H. M. , Neves Welber S. , Kato Massuo Jorge
Published: June 2021

The continuous and indiscriminate use of insecticides has been responsible for the emergence of insecticide resistant vector insect populations, especially in Aedes aegypti. Thus, it is urgent to find natural insecticide compounds with novel mode of action for vector control. The goal of this study was to investigate the larvicidal activity of essential oils (EOs) from Piper species against A. aegypti characterized as resistant and susceptible strains to pyrethroids. The EOs from leaves of 10 Piper species were submitted to the evaluation of larvicidal activity in populations of A. aegypti in agreement with the (World Health Organization, 2005) guidelines. The resistance of the strains characterized by determining the lethal concentrations (LCs) with the insecticide deltamethrin (positive control). The major compounds of the EOs from Piper species was identified by GC-MS. The EOs from Piper aduncum, P. marginatum, P. gaudichaudianum, P. crassinervium, and P. arboreum showed activity of up to 90% lethality at 100 ppm (concentration for screening). The activities of the EOs from these 6 species showed similar LCs in both susceptible strain (Rockefeller) and resistant strains (Pampulha and Venda Nova) to pyrethroids. The major compounds identified in the most active EO were available commercially and included β-Asarone, (E)-Anethole, (E)-β-Caryophyllene, γ-Terpinene, p-Cymene, Limonene, α-Pinene, and β-Pinene. Dillapiole was purified by from EO of P. aduncum. The phenylpropanoids [Dillapiole, (E)-Anethole and β-Asarone] and monoterpenes (γ-Terpinene, p-Cymene, Limonene, α-Pinene, and β-Pinene) showed larvicidal activity with mortality between 90 and 100% and could account for the toxicity of these EOs, but the sesquiterpene (E)-β-Caryophyllene, an abundant component in the EOs of P. hemmendorffii and P. crassinervium, did not show activity on the three populations of A. aegypti larvae at a concentration of 100 ppm. These results indicate that Piper's EOs should be further evaluated as a potential larvicide, against strains resistant to currently used pesticides, and the identification of phenylpropanoids and monoterpenes as the active compounds open the possibility to study their mechanism of action.
Existential Threat: Uncovering Implicit Affect in Response to Terror Reminders in Soldiers
Authors: Quirin Markus , Malekzad Farhood , Kazén Miguel , Luckey Udo , Kehr Hugo
Published: June 2021

Psychological science has a hard time assessing affective processes of the individuals that they may not recognize or do not like to report on. Here, the authors used the Implicit Positive and Negative Affect Test (IPANAT; Quirin et al., 2009) to investigate whether reminders of an existential threat induce unpleasant implicit affect in soldiers waiting for their deployment to a country with high levels of terrorist threat, Afghanistan. As expected, relative to reminding participants of a television evening, implicit negative affect was higher and implicit positive affect was lower after reminding participants of terror acts performed in different cities. No significant effects were found in self-reports of negative or positive affect. Our findings suggest that reminders of existential threat can elicit implicit negative affect that individuals may not report on explicitly and thus, validate the IPANAT as an easily applicable measure in emotional contexts.
Affective Neuroscience: The Suitability of a Web App to Monitor Affective States at Work
Authors: Manfredi Paola , Massardi Elena
Published: June 2021

This work describes in detail the use of a new tool, a web-app, based on the conceptual framework of affective neuroscience, in particular on Panksepp’s 7 basic emotional systems. Affective neuroscience has been used effectively in many areas, but there have been very few applications in the workplace, due to the lack of a smart implementation tool. The novelty of this work does not lie in the new information, but in a new “clinical” approach. There is a theoretical framework that allows data to be interpreted rather than simply described. Furthermore, the knowledge of working realities through the web app is specific and longitudinal. Finally, emotions are detected inhic et nunc, so the role of reflexive-cognitive mediation and recall bias are minor. This “more situated” knowledge can then guide specific leadership strategies. This paper presents the results of the tool’s application in a company in Northern Italy. The findings of our project, which recorded basic affective states and the functioning of several working teams, are detailed herein. The project’s 488 web-app records are summarized in this report, alongside our examination of related mood tags. Through this project, our analysis has enabled to determine affective neuroscience profiles of the teams analyzed, allowing the researchers to identify areas of possible interventions. The data appear very encouraging.
Associations Between C-Reactive Protein Levels, Exercise Addiction, and Athlete Burnout in Endurance Athletes
Authors: Becker Linda , Dupke Anna , Rohleder Nicolas
Published: June 2021

Regular physical activity can support long-term health maintenance, e.g., by reducing inflammatory markers such as C-reactive protein (CRP) levels. However, excessive physical activity can promote the development of both mental and physical illness as well. From a psychological perspective, excessive exercise can lead to the development of exercise addiction (EA) and athlete burnout (AB). However, EA and AB have been rarely investigated so far and it is still unknown whether they are associated with risk factors for physical diseases such as increased CRP levels. In our study, we investigated whether EA and AB in endurance athletes are associated with CRP concentrations. Furthermore, sex differences and prevalence rates of EA were investigated. Ninety-five endurance athletes participated (54.7% female, mean age = 31.8 ± 15.02 years). CRP levels were assessed by means of Dried Blood Spots. For EA and AB assessment, the Exercise Addiction Inventory (EAI) and the Athlete Burnout Questionnaire (ABQ) were used. Exercise addiction was negatively associated with CRP in men. No associations were found for women. None of the ABQ subscales (physical and emotional exhaustion, devaluation of sports, and reduced sense of accomplishment) was associated with CRP levels or with EA. Prevalence of EA was 4.2%. More than 80% of the participants were at risk for EA development. Our results suggest that EA is not necessarily associated with a higher risk for physical diseases through inflammatory pathways. However, EA is a serious mental illness that is widespread in athletes, at least at a subclinical level.
Internet Behavior Preferences Predict Pathological Internet Use: A Latent Profile Analysis
Authors: Chen Jiangtao , Liu Jinmeng , Zhao Gai , Kong Fanchang
Published: June 2021

Recent research in the underlying structure of pathological Internet use (PIU) has produced considerable debate among academics, in which a new “person-centered” approach of studying PIU has recently gathered support but produced mixed results. This study used the latent profile analysis (LPA) to estimate the types of PIU in a large sample of college students (n = 1,400, aged 17–25 years). Participants provided information on demographics, PIU, and Internet behavior preferences. The adolescent pathological Internet use (APIU), which served as the basis of LPA, was used for searching subgroups that represent participants with PIU. LPA identified the PIU classes, and regressions identified the psychological predictors of class membership. Participants were classified into pathological users, pathological-tendency users, preferential users, and ordinary Internet users. In comparison with pathological Internet users, lower Internet gaming and communication preference were associated with pathological-tendency users, preferential users, and ordinary Internet users. The distinct types of college students belong to each PIU class, suggesting that individual differences may be incorporated into the prevention efforts.
Aggression and Psychological Distress in Male and Female Anabolic-Androgenic Steroid Users: A Multigroup Latent Class Analysis
Authors: Chegeni Razieh , Notelaers Guy , Pallesen Ståle , Sagoe Dominic
Published: June 2021

The relationship between anabolic-androgenic steroid (AAS) use and aggression and psychological distress requires further elucidation. No previous study has examined whether the latent patterns of aggression and psychological distress are the same in male and female AAS users. Multigroup latent class analysis (MLCA) can be used to classify individuals into groups based on their responses on a set of variables, and to investigate measurement invariance across subgroups. We therefore conducted a MLCA to identify discrete subgroups of aggression and psychological distress in AAS users, and used measurement invariance to examine whether the identified subgroups can be applied to both sexes. We also examined the relationship between sex and subgroup belongingness. The sample comprised 206 AAS users (females = 58.30%) aged 14–56 (mean = 26.86,SD= 7.12) years. They completed questionnaires assessing demographics, AAS use, aggression, and psychological distress. Based on the MLCA, five subgroups were identified: high aggression moderate distress users (HAMoD: 07.63%), moderate aggression distress users (18.64%), moderate direct aggression-mild indirect aggression moderate distress users (22.95%), mild direct aggression-moderate indirect aggression-distress users (11.71%), and low aggression mild distress users (LAMiD: 39.06%). Although a homogenous five-class solution was the best model for both sexes, sex was significantly associated with the probability of subgroup membership. In particular, members of the HAMoD subgroup were more likely to be male whereas members of the LAMiD subgroup were more likely to be female. Our study provides novel empirical evidence of the idiosyncratic patterns of aggression and psychological distress among male and female AAS users.
Recruitment, Training, and Roles of the Bilingual, Bicultural Navegantes: Developing a Specialized Workforce of Community Health Workers to Serve a Low-Income, Spanish-Speaking Population in Rhode Island
Authors: McCarthy Meghan , Barry Katherine , Estrada Cindy , Veliz Brenda , Rosales Damaris , Leonard Morgan , De Groot Anne S.
Published: June 2021

Clínica Esperanza/Hope Clinic (CEHC) employs Navegantes, who are specially-trained bilingual Community Health Workers (CHW), as key team members who improve the ability of the clinic to provide care for and improve the health status of a large population of uninsured Spanish-speaking patients in Providence, Rhode Island. Given the growing demand for CHWs at the clinic and in the broader healthcare sector in the state, CEHC developed the Advanced Navegante Training Program (ANTP). The ANTP prepares community members to become certified CHWs who are equipped to provide patient navigation and lifestyle coaching as well as professional medical interpretation services. The ANTP is developed and taught by CEHC Navegantes who themselves are bilingual and bicultural peers of trainees as well as the population that CEHC serves. Upon graduation, ANTP trainees have been able to attain higher-paying and fulfilling careers in a range of healthcare and other community settings. The ANTP offers a low-cost, community-based model for training CHWs who are uniquely prepared to promote health and well-being among medically underserved patients.
Wireless Communication for Flying Cars
Authors: Saeed Nasir , Al-Naffouri Tareq Y. , Alouini Mohamed-Slim
Published: June 2021

Current ground-based transportation systems are subjected to various challenges, including the high cost of infrastructure development, limited land space, and a growing urban population. Therefore, the automotive and aviation industries are collaborating to develop flying cars, also known as electric, vertical, takeoff, and landing aircrafts (eVTOLs). These eVTOLs will allow for rapid and reliable urban and suburban transportation. Safe operation of eVTOLs will require well-developed wireless communication networks; however, existing communication technologies need enhancement in order to provide services to flying cars. We describe several potential innovations that make communication between eVTOLs and the ground feasible. These innovations include three-dimensional cellular networks on-ground, tethered balloons, high-altitude platforms, and satellites.
Virtual, Real or Mixed: How Surrounding Objects Influence the Sense of Embodiment in Optical See-Through Experiences?
Authors: Genay Adélaïde , Lécuyer Anatole , Hachet Martin
Published: June 2021

This paper studies the sense of embodiment of virtual avatars in Mixed Reality (MR) environments visualized with an Optical See-Through display. We investigated whether the content of the surrounding environment could impact the user’s perception of their avatar, when embodied from a first-person perspective. To do so, we conducted a user study comparing the sense of embodiment toward virtual robot hands in three environment contexts which included progressive quantities of virtual content: real content only, mixed virtual/real content, and virtual content only. Taken together, our results suggest that users tend to accept virtual hands as their own more easily when the environment contains both virtual and real objects (mixed context), allowing them to better merge the two “worlds”. We discuss these results and raise research questions for future work to consider.
Off-The-Shelf Stylus: Using XR Devices for Handwriting and Sketching on Physically Aligned Virtual Surfaces
Authors: Kern Florian , Kullmann Peter , Ganal Elisabeth , Korwisi Kristof , Stingl René , Niebling Florian , Latoschik Marc Erich
Published: June 2021

This article introduces the Off-The-Shelf Stylus (OTSS), a framework for 2D interaction (in 3D) as well as for handwriting and sketching with digital pen, ink, and paper on physically aligned virtual surfaces in Virtual, Augmented, and Mixed Reality (VR, AR, MR: XR for short). OTSS supports self-made XR styluses based on consumer-grade six-degrees-of-freedom XR controllers and commercially available styluses. The framework provides separate modules for three basic but vital features: 1) The stylus module provides stylus construction and calibration features. 2) The surface module provides surface calibration and visual feedback features for virtual-physical 2D surface alignment using our so-called 3ViSuAl procedure, and surface interaction features. 3) The evaluation suite provides a comprehensive test bed combining technical measurements for precision, accuracy, and latency with extensive usability evaluations including handwriting and sketching tasks based on established visuomotor, graphomotor, and handwriting research. The framework’s development is accompanied by an extensive open source reference implementation targeting the Unity game engine using an Oculus Rift S headset and Oculus Touch controllers. The development compares three low-cost and low-tech options to equip controllers with a tip and includes a web browser-based surface providing support for interacting, handwriting, and sketching. The evaluation of the reference implementation based on the OTSS framework identified an average stylus precision of 0.98 mm (SD= 0.54 mm) and an average surface accuracy of 0.60 mm (SD= 0.32 mm) in a seated VR environment. The time for displaying the stylus movement as digital ink on the web browser surface in VR was 79.40 ms on average (SD= 23.26 ms), including the physical controller’s motion-to-photon latency visualized by its virtual representation (M= 42.57 ms,SD= 15.70 ms). The usability evaluation (N= 10) revealed a low task load, high usability, and high user experience. Participants successfully reproduced given shapes and created legible handwriting, indicating that the OTSS and it’s reference implementation is ready for everyday use. We provide source code access to our implementation, including stylus and surface calibration and surface interaction features, making it easy to reuse, extend, adapt and/or replicate previous results (https://go.uniwue.de/hci-otss).
Talent Identification in Youth Soccer: Prognosis of U17 Soccer Performance on the Basis of General Athleticism and Talent Promotion Interventions in Second-Grade Children
Authors: Hohmann Andreas , Siener Maximilian
Published: June 2021

Several talent identification programs in elementary school have implemented motor diagnostics to introduce children to groups of sports, like game sports, or even to particular sports like soccer. However, as in most other sports, in youth soccer, the predictive value of such early testing is still unclear. This prospective study evaluated the midterm prognostic validity of generic motor performance tests. The sample consisted of male second-grade children, which had received a recommendation to participate in soccer. The talent screening campaign was a basic check comprising two anthropometric parameters, five physical fitness, and three motor competence diagnostics of the German Motor Test 6–18. The test data were collected from the participating elementary school classes of the years 2010 to 2014. The soccer competition performance of those children having completed the age of at least 15 years (n = 502) up to the end of the season 2019/2020 (2020, September 30) was recorded. This group of U17 players was then assigned individually to five different competition levels. The prognostic validity of the physical and physiological tests was determined using ANOVAs, odds ratios, and a regression path analysis. All diagnostic methods exhibited medium-to-high prognostic validity over the 8 year time span from the talent screening to the later soccer competitions in the adolescent age groups. For later success in soccer on the province level, the 6-min run (OR = 4.28), dynamic balance (OR = 4.04), and 20-m sprint (OR = 2.46), as well as the participation in the training center of the German Soccer Federation (OR = 5.67) and the diversity of club sport activities (OR = 3.56), were of particular importance.
Concussion Reporting and Safeguarding Policy Development in British American Football: An Essential Agenda
Authors: Travis Eleanor , Thornton Claire , Scott-Bell Andrea
Published: June 2021

The objective of this study was to examine concussion reporting and safeguarding policy in British American Football (BAF). Data were collected via an online survey tool. The data presented are part of a broader study that examined injury profiles, concussion reporting behaviors, and medical provision in BAF. Concussion-like symptoms were found in over half (58.8%) of the participants. Of those, 36.4% reported they had previously been formally diagnosed with a concussion while playing BAF. Just under half of the participants (44.7%) had suspected they had had a concussion, although it was not formally diagnosed, and 23.5% of the participants had previously hidden concussion symptoms. Fifty-eight percent of the teams reported they did not have a regular game-day medic, with a range of hired medical personnel who attended the games. Prominent barriers to hiring a medic included budget, institutional support shortfall, and lack of medic reliability and game knowledge. BAF is a developing sport with a clear vision for growth of participation. Yet, the current concussion and medical provision policies do not address the sport's welfare needs. Through discussion of these policies in the context of this study's findings, we highlight vital areas of concern in policy and practice that the British American Football Association needs to address in their medical and concussion policies.
Surgical Diagnosis and Treatment of Primary Retroperitoneal Liposarcoma
Authors: Chen Jie , Hang Ying , Gao Qi , Huang Xinyu
Published: June 2021

Background:Primary retroperitoneal liposarcoma (PRPLS) is the most common soft tissue sarcoma of the retroperitoneum with high recurrence rate and short overall survival (OS).

Methods:A retrospective review of 51 patients with PRPLS, treated between September 1, 2009 and November 30, 2020, was conducted to evaluate clinical outcomes of PRPLS resection. Patient demographics, histopathologic subtypes, overall survival (OS), progression-free survival (PFS), disease recurrence rate, and tumor stage were reviewed and analyzed. Univariate analysis was done to identify factors potentially affecting OS and PFS of PRPLS patients. Multivariate Cox proportional hazards analysis was used to evaluate the impact of various clinicopathological factors on OS and PFS of PRPLS patients.

Results:Fifty-one PRPLS patients (28 Males, 23 Females; mean age 56.25 years) were evaluated. There was no significant effect of age, gender, contiguous organ resection, degree of differentiation and tumor size on the OS and PFS of the patients. Univariate analysis showed that negative surgical margin and early tumor stage significantly correlated with OS and PFS (allP< 0.001). Multivariate analysis showed that tumor stage [hazard ratio (HR) = 1.177,P= 0.001] was an independent predictors of poor progression-free survival, and surgical margins [HR = 4.0674P= 0.038] and tumor stage [HR = 1.167P= 0.001] were identified as independent predictors of poor overall survival.

Conclusion:Negative surgical margin is a prognostic factor of OS, and can prolong the postoperative survival time of PRPLS patients. Tumor stage is a prognostic factor for OS and PFS, and can influence the survival of PRPLS patients. Earlier tumor stages of PRPLS are associated with significantly better outcomes.
Miscanthus Grass as a Novel Functional Fiber Source in Extruded Feline Diets
Authors: Finet Shannon E. , Southey Bruce R. , Rodriguez-Zas Sandra L. , He Fei , de Godoy Maria R. C.
Published: June 2021

Although dietary fiber is not considered an essential nutrient in a complete and balanced diet for felines, it provides a substrate for fermentation by gut microbiota, thus promoting gastrointestinal health through the production of fermentative metabolites, as well as improving laxation. The aim of this research was to evaluate the novel fiber source,Miscanthusgrass (Miscanthus giganteus), in comparison with traditional fiber sources and their effects on fecal quality, apparent total tract digestibility (ATTD), fecal fermentative end products, and microbiota of healthy adult cats. Four dietary treatments were evaluated, differing in dietary fiber source. The diets were formulated to meet or exceed the AAFCO (2018) nutritional profile for adult cats and contained either cellulose (CO),Miscanthusgrass fiber (MF), a blend ofMiscanthusfiber and tomato pomace (MF + TP), or beet pulp (BP). The study was conducted using a completely randomized design with 28 neutered adult, domesticated shorthair cats (19 females and 9 males, mean age 2.2 ± 0.03 years; mean body weight 4.6 ± 0.7 kg, mean body condition score 5.6 ± 0.6). The experimental period comprised 21 days, and a fresh fecal and a total fecal collection were performed during the last 4 days of the trial period. Daily food intake (DM basis) was similar across all groups (P> 0.05). Additionally, treatment did not affect fecal output (as-is or DM basis), fecal score, or fecal pH (P> 0.05). Cats fed BP had significantly higher total dietary fiber ATTD than all the other treatments (P< 0.05) and the highest concentrations of total short-chain fatty acid, acetate, and propionate (P< 0.05), while butyrate concentrations were similar for all treatments (P> 0.05). Inclusion of dietary fibers was effective in modulating gut microbiota. Cats fed diets containingMiscanthusgrass had greater α-diversity than cats fed BP. As no adverse effects on health, fecal quality, or ATTD of macronutrients were observed with the inclusion of 9%Miscanthusgrass fiber or fiber blend, the data suggest thatMiscanthusgrass fiber and fiber blends are viable alternatives to the traditional dietary fiber sources used in commercial extruded feline diets, being most comparable to cellulose.
Pigeons (Columba livia domestica) Are Susceptible to Infectious Bursal Disease: A Comparative Study of Their Hematological and Serum Biochemical Alterations
Authors: Orakpoghenor Ochuko , Oladele Sunday Blessing , Abdu Paul Ayuba , Markus Talatu Patience , Enam Samson James , Andamin Aliyu Danlami , Muhammed Muhammed Shuaib , Usman Suleman Geidam , Esievo King Akpofure Nelson
Published: June 2021

The hematological and serum biochemical alterations following very virulent infectious bursal disease virus (IBDV) infection in pigeons and chickens were evaluated in this study. Sixty IBDV seronegative birds comprising 30 (3–6 weeks old) pigeons and 30 (3 weeks old) chickens were randomly divided as follows: 10 uninoculated pigeons only, 10 inoculated pigeons + 10 sentinel chickens, 10 inoculated chickens + 10 sentinel pigeons, and 10 uninoculated chickens. Inoculated birds were administered 0.20 ml of inoculum containing very virulent IBDV (vvIBDV). Blood was collected postinoculation/exposure (pi/pe) and processed for hematology and biochemistry. The results revealed significantly (P < 0.05) increased packed cell volume, decreased mean corpuscular hemoglobin (MCH), and MCH concentration (MCHC) in inoculated and sentinel chickens. Total leukocyte count (TLC), heterophil, and heterophil/lymphocyte (H/L) ratio were increased from 3 dpi/dpe in inoculated pigeons and from 3 to 14 dpi/dpe in inoculated and sentinel chickens. At 10 and 14 dpi/dpe, there was significantly (P < 0.05) increased serum total protein and globulin concentrations and decreased albumin/globulin ratio in pigeons and chickens. Serum urea concentration showed significant (P < 0.05) increase in inoculated and sentinel chickens at 3 and 4 dpi/dpe. To the authors' knowledge, this is the first report on hematological and serum biochemical alterations due to vvIBDV infection in pigeons.
An Intelligent Actuator of an Indoor Logistics System Based on Multi-Sensor Fusion
Authors: Wang Pangwei , Wang Yunfeng , Wang Xu , Liu Ying , Zhang Juan
Published: June 2021

Integration technologies of artificial intelligence (AI) and autonomous vehicles play important roles in intelligent transportation systems (ITS). In order to achieve better logistics distribution efficiency, this paper proposes an intelligent actuator of an indoor logistics system by fusing multiple involved sensors. Firstly, an actuator based on a four-wheel differential chassis is equipped with sensors, including an RGB camera, a lidar and an indoor inertial navigation system, by which autonomous driving can be realized. Secondly, cross-floor positioning can be realized by multi-node simultaneous localization and mappings (SLAM) based on the Cartographer algorithm Thirdly the actuator can communicate with elevators and take the elevator to the designated delivery floor. Finally, a novel indoor route planning strategy is designed based on an A* algorithm and genetic algorithm (GA) and an actual building is tested as a scenario. The experimental results have shown that the actuator can model the indoor mapping and develop the optimal route effectively. At the same time, the actuator displays its superiority in detecting the dynamic obstacles and actively avoiding the collision in the indoor scenario. Through communicating with indoor elevators, the final delivery task can be completed accurately by autonomous driving.
Design of Software-Defined Radio-Based Adaptable Packet Communication System for Small Satellites
Authors: ABBAS Yasir M. O. , Asami Kenichi
Published: June 2021

Software-defined radio (SDR) devices have made a massive contribution to communication systems by reducing the cost and development time for radio frequency (RF) designs. SDRs opened the gate to programmers and enabled them to increase the capabilities of these easily manipulated systems. The next step is to upgrade the reconfigurability into adaptability, which is the focus of this paper. This research contributes to improving SDR-based systems by designing an adaptable packet communication transmitter and receiver that can utilize the communication window of CubeSats and small satellites. According to the feedback from the receiver, the transmitter modifies the characteristics of the signal. Theoretically, the system can adopt many modes, but for simplicity and to prove the concept, here, the changes are limited to three data rates of the Gaussian minimum shift keying (GMSK) modulation scheme, i.e., 2400 bps GMSK, 4800 bps GMSK and 9600 bps GMSK, which are the most popular in amateur small satellites. The system program was developed using GNU Radio Companion (GRC) software and Python scripts. With the help of GRC software, the design was simulated and its behavior in simulated conditions observed. The transmitter packetizes the data into AX.25 packets and transmits them in patches. Between these patches, it sends signaling packets. The patch size is preselected. Alternatively, the receiver extracts the data and saves it in a dedicated file. It directly replies with a feedback message whenever it gets the signaling packets. Based on the content of the feedback message, the characteristics of the transmitted signal are altered. The packet rate and the actual useful data rate are measured and compared with the selected data rate, and the packet success rate of the system operating at a fixed data rate is also measured while simulating channel noise to achieve the desired Signal-to-Noise Ratio (SNR).
Winter Wheat Cultivar Recommendation Based on Expected Environment Productivity
Authors: Iwańska Marzena , Paderewski Jakub , Stępień Michał , Rodrigues Paulo Canas
Published: June 2021

We used 5 years of data from multi-environmental trials conducted in Poland to assess average winter wheat yield based on selected environmental factors to recommend cultivars depending on their performance in environments of different productivity. Average expected yields in particular environments were calculated using a model based on analysis of covariance (ANCOVA), which describes the relationship between winter wheat yield and environmental factors of soil suitability and pH, drought length and Selyaninov’s Hydrothermal Coefficient (HTC) in 10-day periods. The cultivar performance was evaluated using linear regression. The cultivar yield estimated by the mixed model was considered the dependent variable, whereas the environmental mean yields, estimated by ANCOVA, were considered independent variables. The cultivars were ranked according to the estimated yield in environments of determined average wheat productivity. Higher yielding cultivars were divided into two groups: widely and narrowly adapted cultivars, which were then recommended. The novelty of this study stems from the consideration of the environmental productivity in the recommendation process, the indication of widely adapted cultivars to be grown in a broad range of productivity sites and the selection of cultivars with narrow adaptation, which may outperform cultivars of wide adaptation in homogeneous fields. This study confirmed the importance of soil suitability and HTC for winter wheat yield. Direct application of our results is possible in Poland and in other countries with similar conditions.
Genome-Wide SNP Analysis for Milk Performance Traits in Indigenous Sheep: A Case Study in the Egyptian Barki Sheep
Authors: Abousoliman Ibrahim , Reyer Henry , Oster Michael , Murani Eduard , Mohamed Ismail , Wimmers Klaus
Published: June 2021

Sheep milk yield and milk composition traits play an important role in supplying newborn lambs with essential components such as amino acids, energy, vitamins and immune antibodies and are also of interest in terms of the nutritional value of the milk for human consumption. The aim of this study was to identify genomic regions and candidate genes for milk yield and milk composition traits through genome-wide SNP analyses between high and low performing ewes of the Egyptian Barki sheep breed, which is well adapted to the harsh conditions of North-East Africa. Therefore, out of a herd of 111 ewes of the Egyptian Barki sheep breed (IBD = 0.08), ewes representing extremes in milk yield and milk quality traits (n = 25 for each group of animals) were genotyped using the Illumina OvineSNP50 V2 BeadChip. The fixation index (FST) for each SNP was calculated between the diversified groups. FST values were Z-transformed and used to identify putative SNPs for further analysis (Z(FST) > 10). Genome-wide SNP analysis revealed genomic regions covering promising candidate genes related to milk performance traits such as SLC5A8, NUB1, TBC1D1, KLF3 and ABHD5 for milk yield and PPARA and FBLN1 genes for milk quality trait. The results of this study may contribute to the genetic improvement of milk performance traits in Barki sheep breed and to the general understanding of the genetic contribution to variability in milk yield and quality traits.
The Capacity of Holstein-Friesian and Simmental Cows to Correct a Negative Energy Balance in Relation to Their Performance Parameters, Course of Lactation, and Selected Milk Components
Authors: Strączek Ilona , Młynek Krzysztof , Danielewicz Agata
Published: June 2021

A significant factor in improving the performance of dairy cows is their physiological ability to correct a negative energy balance (NEB). This study, using Simmental (SIM) and Holstein-Friesian (HF) cows, aimed to assess changes in NEB (non-esterified fatty acid; body condition score; and C16:0, C18:0, and C18:1) and its effect on the metabolic efficiency of the liver (β-hydroxybutyrate and urea). The effects of NEB on daily yield, production at peak lactation and its duration, and changes in selected milk components were assessed during complete lactation. Up to peak lactation, the loss of the body condition score was similar in both breeds. Subsequently, SIM cows more efficiently restored their BCS. HF cows reached peak lactation faster and with a higher milk yield, but they were less able to correct NEB. During lactation, their non-esterified fatty acid, β-hydroxybutyrate, C16:0, C18:0, C18:1, and urea levels were persistently higher, which may indicate less efficient liver function during NEB. The dynamics of NEB were linked to levels of leptin, which has anorectic effects. Its content was usually higher in HF cows and during intensive lactogenesis. An effective response to NEB may be exploited to improve the production and nutritional properties of milk. In the long term, it may extend dairy cows’ productive life and increase lifetime yield.
Training Pigs for Oral Glucose Tolerance Test—Six Years’ Experience of a Refined Model
Authors: Manell Elin , Hedenqvist Patricia , Jensen-Waern Marianne
Published: June 2021

Animal models of human diseases are important in biomedical research. When using animals for scientific purposes, the 3Rs (replace, reduce, refine) should be considered. Refinement of animal models is essential to ensure best use of animals, which is important for ethical reasons and to retrieve reliable research data. The present publication describes improvements to an oral glucose tolerance test (OGTT) model for pigs published in 2016. Historical data from 42 pigs were used to describe improvements in the training technique over six years. Pigs of various breeds and ages can be trained to bottle-feed glucose dissolved in water to undergo OGTT. This publication describes different tips and techniques to apply for successful training and will help researchers to minimize exclusions of pigs due to unsuccessful training. The improvements are an important contribution to the 3Rs.
Advances in Bacteriophage Therapy against Relevant MultiDrug-Resistant Pathogens
Authors: Broncano-Lavado Antonio , Santamaría-Corral Guillermo , Esteban Jaime , García-Quintanilla Meritxell
Published: June 2021

The increase of multiresistance in bacteria and the shortage of new antibiotics in the market is becoming a major public health concern. The World Health Organization (WHO) has declared critical priority to develop new antimicrobials against three types of bacteria: carbapenem-resistant A. baumannii, carbapenem-resistant P. aeruginosa and carbapenem-resistant and ESBL-producing Enterobacteriaceae. Phage therapy is a promising alternative therapy with renewed research in Western countries. This field includes studies in vitro, in vivo, clinical trials and clinical cases of patients receiving phages as the last resource after failure of standard treatments due to multidrug resistance. Importantly, this alternative treatment has been shown to be more effective when administered in combination with antibiotics, including infections with biofilm formation. This review summarizes the most recent studies of this strategy in animal models, case reports and clinical trials to deal with infections caused by resistant A. baumannii, K. pneumoniae, E. coli, and P. aeruginosa strains, as well as discusses the main limitations of phage therapy.
Association of Antimicrobial Resistance in Campylobacter spp. in Broilers and Turkeys with Antimicrobial Use
Authors: Tenhagen Bernd-Alois , Flor Matthias , Alt Katja , Knüver Marie-Theres , Buhler Christiane , Käsbohrer Annemarie , Stingl Kerstin
Published: June 2021

We investigated trends in antimicrobial resistance (AMR) in Campylobacter jejuni and Campylobacter coli in poultry between 2010 and 2016 in Germany and their association with antimicrobial use. Campylobacter had been isolated from the caeca of broilers and turkeys at slaughter by regional laboratories according to current ISO methods in the framework of a national monitoring program. Isolates were submitted to the National Reference Laboratory for Campylobacter and tested for AMR using broth microdilution methods. Minimum inhibitory concentrations were evaluated according to epidemiological cut-off values. Antimicrobial use (AMU) data from 2014 to 2016 were taken from a government report. AMR was higher in C. coli than in C. jejuni and higher in turkeys than in broilers. AMR was highest to tetracycline and the tested (fluoro)quinolones while it was rare to gentamicin in both bacterial species, infrequent to erythromycin in C. jejuni, and moderate in C. coli. AMR to tetracycline and erythromycin decreased over time while it increased to (fluoro)quinolones. An association of AMU and AMR was observed for resistance to tetracycline and erythromycin, while it was not observed for the aminoglycosides. Resistance to nalidixic acid and ciprofloxacin increased despite a decrease of fluoroquinolone use between 2014 and 2016, indicating that other factors have a strong influence on resistance to (fluoro)quinolones in Campylobacter.
Accumulation of Seminolipid in Sertoli Cells Is Associated with Increased Levels of Reactive Oxygen Species and Male Subfertility: Studies in Aging Arsa Null Male Mice
Authors: Kongmanas Kessiri , Saewu Arpornrad , Kiattiburut Wongsakorn , Baker Mark A , Faull Kym F , Burger Dylan , Tanphaichitr Nongnuj
Published: June 2021

Seminolipid (also known as sulfogalactosylglycerolipid-SGG), present selectively in male germ cells, plays important roles in spermatogenesis and sperm–egg interaction. The proper degradation of SGG in apoptotic germ cells is also as important. Sertoli cells first phagocytose apoptotic germ cells, then Sertoli lysosomal arylsulfatase A (ARSA) desulfates SGG, the first step of SGG degradation. We have reported that aging male Arsa−/− mice become subfertile with SGG accumulation in Sertoli cell lysosomes, typical of a lysosomal storage disorder (LSD). Since reactive oxygen species (ROS) levels are increased in other glycolipid-accumulated LSDs, we quantified ROS in Arsa−/− Sertoli cells. Our analyses indicated increases in superoxide and H2O2 in Arsa−/− Sertoli cells with elevated apoptosis rates, relative to WT counterparts. Excess H2O2 from Arsa−/− Sertoli cells could travel into testicular germ cells (TGCs) to induce ROS production. Our results indeed indicated higher superoxide levels in Arsa−/− TGCs, compared with WT TGCs. Increased ROS levels in Arsa−/− Sertoli cells and TGCs likely caused the decrease in spermatogenesis and increased the abnormal sperm population in aging Arsa−/− mice, including the 50% decrease in sperm SGG with egg binding ability. In summary, our study indicated that increased ROS production was the mechanism through which subfertility manifested following SGG accumulation in Sertoli cells.
Review of Propulsion System Design Strategies for Unmanned Aerial Vehicles
Authors: Amici Cinzia , Ceresoli Federico , Pasetti Marco , Saponi Matteo , Tiboni Monica , Zanoni Simone
Published: June 2021

The design of the propulsion system for Unmanned Aerial Vehicles (UAVs) demands an inclusive multidisciplinary approach from the earliest design phases, since every design choice strictly affects and is affected by the overall working conditions. This paper presents a review of the scientific literature focused on the design methods applied in defining and sizing the propulsion system of drones. The analysis, performed with a systematic approach, evaluated 123 papers according to two custom classification taxonomies, which investigated respectively the primary aim and specific content of the works. Finally, literature indications and hints were combined into an integrated framework for the functional design of the propulsion system of UAVs. The procedure aimed to support the designer in the preliminary selection of the propulsion candidates and the quick sizing of the supply system, during the first phases of the design process. According to the literature, design methods dramatically change depending on the expected applications and working conditions of UAVs, so that the detailed design of specific drone elements and propulsion components represents the focus of most of the papers in this field.
Decision Making with STPA through Markov Decision Process, a Theoretic Framework for Safe Human-Robot Collaboration
Authors: Zacharaki Angeliki , Kostavelis Ioannis , Dokas Ioannis
Published: June 2021

During the last decades, collaborative robots capable of operating out of their cages are widely used in industry to assist humans in mundane and harsh manufacturing tasks. Although such robots are inherently safe by design, they are commonly accompanied by external sensors and other cyber-physical systems, to facilitate close cooperation with humans, which frequently render the collaborative ecosystem unsafe and prone to hazards. We introduce a method that capitalizes on partially observable Markov decision processes (POMDP) to amalgamate nominal actions of the system along with unsafe control actions posed by the System Theoretic Process Analysis (STPA). A decision-making mechanism that constantly prompts the system into a safer state is realized by providing situation awareness about the safety levels of the collaborative ecosystem by associating the system safety awareness with specific groups of selected actions. POMDP compensates the partial observability and uncertainty of the current state of the collaborative environment and creates safety screening policies that tend to make decisions that balance the system from unsafe to safe states in real time during the operational phase. The theoretical framework is assessed on a simulated human–robot collaborative scenario and proved capable of identifying loss and success scenarios.
Detection of Unknown DDoS Attacks with Deep Learning and Gaussian Mixture Model
Authors: Shieh Chin-Shiuh , Lin Wan-Wei , Nguyen Thanh-Tuan , Chen Chi-Hong , Horng Mong-Fong , Miu Denis
Published: June 2021

DDoS (Distributed Denial of Service) attacks have become a pressing threat to the security and integrity of computer networks and information systems, which are indispensable infrastructures of modern times. The detection of DDoS attacks is a challenging issue before any mitigation measures can be taken. ML/DL (Machine Learning/Deep Learning) has been applied to the detection of DDoS attacks with satisfactory achievement. However, full-scale success is still beyond reach due to an inherent problem with ML/DL-based systems—the so-called Open Set Recognition (OSR) problem. This is a problem where an ML/DL-based system fails to deal with new instances not drawn from the distribution model of the training data. This problem is particularly profound in detecting DDoS attacks since DDoS attacks’ technology keeps evolving and has changing traffic characteristics. This study investigates the impact of the OSR problem on the detection of DDoS attacks. In response to this problem, we propose a new DDoS detection framework featuring Bi-Directional Long Short-Term Memory (BI-LSTM), a Gaussian Mixture Model (GMM), and incremental learning. Unknown traffic captured by the GMM are subject to discrimination and labeling by traffic engineers, and then fed back to the framework as additional training samples. Using the data sets CIC-IDS2017 and CIC-DDoS2019 for training, testing, and evaluation, experiment results show that the proposed BI-LSTM-GMM can achieve recall, precision, and accuracy up to 94%. Experiments reveal that the proposed framework can be a promising solution to the detection of unknown DDoS attacks.