bluuebunny/crossref_metadata_2025 huggingface dataset:
A Follow-Up Investigation of Mental Health Among Discharged COVID-19 Patients in Wuhan, China
Authors: Li Li , Wu Michael Shengtao , Tao Junxiu , Wang Weijun , He Jing , Liu Ru , Guo Juan , Chen Yun , Li Kejian , Li Shilong , Qi Bo , Han Buxin
Published: April 2021

Objective: To understand the mental health status and its risk factors among discharged COVID-19 patients during the first month of centralized quarantine and the subsequent home isolation.

Methods: The scales of the Insomnia Severity Index (ISI), General Anxiety Disorder (GAD-7), and Patient Health Questionnaire (PHQ-9) were used to measure the symptoms of insomnia, anxiety, and depression in 782 COVID-19 patients during the first month of centralized quarantine (March 16 to 26, 2020) and then during home isolation (April 3 to 10, 2020).

Results: During the centralized quarantine, the prevalence rates of insomnia, anxiety, and depressive symptoms were 44.37, 31.59, and 27.62%, respectively, and those during the home isolation decreased significantly at 27.11, 17.26, and 16.11%, respectively. In both waves, women showed a higher prevalence of symptoms of poor mental health compared to men, and middle-aged (40–59 years old) and elderly (≥60 years old) showed a higher risk of symptoms of poor mental health compared to the younger. In addition, the severity of COVID-19 revealed no significant relationship to symptoms of poor mental health, whereas, the interaction analysis revealed that those with other underlying diseases showed more symptoms of poor mental health during the centralized quarantine and a greater decrease during the follow-up home isolation.

Conclusion: The discharged COVID-19 patients suffered from mental health problems such as, insomnia, depression, and anxiety, and this was especially so for women, the middle-aged and elderly, and those with underlying diseases, but along with the rehabilitation and the environmental change from centralized quarantine to home isolation, all the mental symptoms were significantly alleviated. Based on a follow-up investigation, the current results provide critical evidence for mental health and early rehabilitation upon the discharged COVID-19 patients.
AQuRo: A Cat-like Adaptive Quadruped Robot With Novel Bio-Inspired Capabilities
Authors: Saputra Azhar Aulia , Takesue Naoyuki , Wada Kazuyoshi , Ijspeert Auke Jan , Kubota Naoyuki
Published: April 2021

There are currently many quadruped robots suited to a wide range of applications, but traversing some terrains, such as vertical ladders, remains an open challenge. There is still a need to develop adaptive robots that can walk and climb efficiently. This paper presents an adaptive quadruped robot that, by mimicking feline structure, supports several novel capabilities. We design a novel paw structure and several point-cloud-based sensory structures incorporating a quad-composite time-of-flight sensor and a dual-laser range finder. The proposed robot is equipped with physical and cognitive capabilities which include: 1) a dynamic-density topological map building with attention model, 2) affordance perception using the topological map, and 3) a neural-based locomotion model. The novel capabilities show strong integration between locomotion and internal–external sensory information, enabling short-term adaptations in response to environmental changes. The robot performed well in several situations: walking on natural terrain, walking with a leg malfunction, avoiding a sudden obstacle, climbing a vertical ladder. Further, we consider current problems and future development.
A Flexible Transoral Robot Towards COVID-19 Swab Sampling
Authors: Li Changsheng , Gu Xiaoyi , Xiao Xiao , Lim Chwee Ming , Duan Xingguang , Ren Hongliang
Published: April 2021

There are high risks of infection for surgeons during the face-to-face COVID-19 swab sampling due to the novel coronavirus’s infectivity. To address this issue, we propose a flexible transoral robot with a teleoperated configuration for swab sampling. The robot comprises a flexible manipulator, an endoscope with a monitor, and a master device. A 3-prismatic-universal (3-PU) flexible parallel mechanism with 3 degrees of freedom (DOF) is used to realize the manipulator’s movements. The flexibility of the manipulator improves the safety of testees. Besides, the master device is similar to the manipulator in structure. It is easy to use for operators. Under the guidance of the vision from the endoscope, the surgeon can operate the master device to control the swab’s motion attached to the manipulator for sampling. In this paper, the robotic system, the workspace, and the operation procedure are described in detail. The tongue depressor, which is used to prevent the tongue’s interference during the sampling, is also tested. The accuracy of the manipulator under visual guidance is validated intuitively. Finally, the experiment on a human phantom is conducted to demonstrate the feasibility of the robot preliminarily.
Cognitive Intervention Through Photo-Integrated Conversation Moderated by Robots (PICMOR) Program: A Randomized Controlled Trial
Authors: Otake-Matsuura Mihoko , Tokunaga Seiki , Watanabe Kumi , Abe Masato S. , Sekiguchi Takuya , Sugimoto Hikaru , Kishimoto Taishiro , Kudo Takashi
Published: April 2021

Social interaction might prevent or delay dementia, but little is known about the specific effects of various social activity interventions on cognition. This study conducted a single-site randomized controlled trial (RCT) of Photo-Integrated Conversation Moderated by Robots (PICMOR), a group conversation intervention program for resilience against cognitive decline and dementia. In the RCT, PICMOR was compared to an unstructured group conversation condition. Sixty-five community-living older adults participated in this study. The intervention was provided once a week for 12 weeks. Primary outcome measures were the cognitive functions; process outcome measures included the linguistic characteristics of speech to estimate interaction quality. Baseline and post-intervention data were collected. PICMOR contains two key features: 1) photos taken by the participants are displayed and discussed sequentially; and 2) a robotic moderator manages turn-taking to make sure that participants are allocated the same amount of time. Among the primary outcome measures, one of the subcategories of cognitive functions, verbal fluency significantly improved in the intervention group. Among the process outcome measures, a part of the subcategories of linguistic characteristics of speech, the amount of speech and richness of words, proportion of providing topics, questions, and answers in total utterances were larger for the intervention group. This study demonstrated for the first time the positive effects of a robotic social activity intervention on cognitive function in healthy older adults via RCT. The group conversation generated by PICMOR may improve participants’ verbal fluency since participants have more opportunity to provide their own topics, asking and answering questions which results in exploring larger vocabularies. PICMOR is available and accessible to community-living older adults.

Clinical Trial Registration:UMIN Clinical Trials Registry, identifier UMIN000036667.
Travelling Santa Problem: Optimization of a Million-Households Tour Within One Hour
Authors: Strutz Tilo
Published: April 2021

Finding the shortest tour visiting all given points at least ones belongs to the most famous optimization problems until today [travelling salesman problem (TSP)]. Optimal solutions exist for many problems up to several ten thousand points. The major difficulty in solving larger problems is the required computational complexity. This shifts the research from finding the optimum with no time limitation to approaches that find good but sub-optimal solutions in pre-defined limited time. This paper proposes a new approach for two-dimensional symmetric problems with more than a million coordinates that is able to create good initial tours within few minutes. It is based on a hierarchical clustering strategy and supports parallel processing. In addition, a method is proposed that can correct unfavorable paths with moderate computational complexity. The new approach is superior to state-of-the-art methods when applied to TSP instances with non-uniformly distributed coordinates.
Protecting Our Climate by Turning CO2 Into Stone
Authors: Snæbjörnsdóttir Sandra Ó. , Steinþórsdóttir Katrín , Snorradóttir Selja Ósk , Helgason Kári
Published: April 2021

The biggest task of this century is to reduce the emissions of CO2 into the atmosphere, to slow down climate change. One key technology is to capture the CO2 from power plants and factories to prevent it from affecting the climate. But how can we store this captured CO2 in a safe and permanent manner? In this article, we report on a method that has been demonstrated in Iceland, in which CO2 is injected deep into the ground and turned into stone—a natural way of dealing with CO2 emissions!
“Back to Where They Were”: The Socio-Discursive Representation of Transgender Sex Workers and Urban Space in a Television News Report
Authors: Soich Matías
Published: April 2021

Despite significant advances in recent years, Argentina’s transgender community still faces structural social exclusion. For a vast majority of transvestites and transgender women, early expulsion from the family home and the educational system results in having to resort to prostitution as their only option for surviving. Police edicts and other similar devices are used to penalize prostitution and persecute transgender people in public places, showing that prejudice and violence against their identities also manifest in the control of urban space. Here I present the results of an in-depth qualitative linguistic analysis of a 2018 television news report about the temporary relocation of the transgender sex workers from their usual location in the Bosques de Palermo, the biggest public park in the City of Buenos Aires. The theoretical frame is Critical Discourse Analysis and the methodology is inductive and qualitative. The analysis centers on the linguistic resources that define the socio-discursive representation about the transgender sex workers in relation with urban space and the city’s government. The bases of the analysis are the Synchronic-Diachronic Method for the Linguistic Analysis of Texts and the Method of Converging Linguistic Approaches. These methods revealed, in the first place, that the transvestites and transgender women are represented as mere occupants of public space through their close association with the discursive category of Space. In the second place, they are represented as fundamentally passive in relation to the Government of the City of Buenos Aires; while, at the same time, the government’s responsibility for their displacement is systematically mitigated. Finally, the lack of work alternatives to prostitution for the transgender community is naturalized through the persistent association of the discursive categories connected with transgender people, prostitution and urban space. If we compare these results with those of previous research, we can see that these discursive features—none of which challenge the status quo—remain one of the basic components of the socio-discursive representation of transgender people elaborated by the mainstream media.
High Resolution Soft X-ray Spectroscopy and the Quest for the Hot (5–10 MK) Plasma in Solar Active Regions
Authors: Del Zanna Giulio , Andretta Vincenzo , Cargill Peter J. , Corso Alain J. , Daw Adrian N. , Golub Leon , Klimchuk James A. , Mason Helen E.
Published: April 2021

We discuss the diagnostics available to study the 5–10 MK plasma in the solar corona, which is key to understanding the heating in the cores of solar active regions. We present several simulated spectra, and show that excellent diagnostics are available in the soft X-rays, around 100 Å, as six ionization stages of Fe can simultaneously be observed, and electron densities derived, within a narrow spectral region. As this spectral range is almost unexplored, we present an analysis of available and simulated spectra, to compare the hot emission with the cooler component. We adopt recently designed multilayers to present estimates of count rates in the hot lines, with a baseline spectrometer design. Excellent count rates are found, opening up the exciting opportunity to obtain high-resolution spectroscopy of hot plasma.
Frequency, Stressfulness and Type of Ethically Challenging Situations Encountered by Veterinary Team Members During the COVID-19 Pandemic
Authors: Quain Anne , Mullan Siobhan , McGreevy Paul D. , Ward Michael P.
Published: April 2021

Ethically challenging situations (ECS) are common in veterinary settings and can lead to moral stress. However, there is no published information about how a global pandemic affects the frequency and types of ECS encountered by veterinary team members. An online mixed methods survey was developed to determine the frequency, stressfulness and types of ECS experienced by veterinarians, animal health technicians and veterinary nurses since the advent of the global COVID-19 pandemic in March 2020. Responses from 540 veterinary team members from 22 countries were analyzed. With the advent of the COVID-19 pandemic, the median frequency of ECS encountered by respondents increased from several times per month to several times per week (Spearman Rank Correlation 0.619, P < 0.0001). The most common ECS (encountered at least several times per week) were: challenging decisions about how to proceed when clients have limited finances (64.4%), conflict between personal well-being and professional role (64.3%), conflict between the interests of clients and the interests of their animals (59.6%). These were followed by challenging decisions about what counts as an essential veterinary service (48.1%); conflict between well-being of family/household members and professional role (46.3%); and challenging decisions about whether to perform non-contact veterinary visits (46.3%). The most stressful ECS (reported to be very or maximally stressful) were: conflicts between the interests of clients and the interests of their animals (50.2%), other (42.9%), conflicts between the interests of my employer and my own interests (42.5%), challenging decisions about how to proceed when clients have limited finances (39.4%), conflict between personal well-being and professional role (38.0%), and conflict between well-being of family/household members and professional role (33.6%). Thematic analysis of free-text responses revealed biosecurity, client financial limitations, animal welfare, working conditions, and client relations as prominent themes. This is, to the best of our knowledge, the first study to describe the impacts of the pandemic on ECS experienced by veterinary teams globally. It identifies an increase in the frequency of ECS associated with the COVID-19 pandemic, and a number of stressors unique to the pandemic. We identified a number of resources and strategies that may help veterinary team members navigate ethical challenges that may emerge in their daily work, as well as in the context of global crises.
Global Visualization of Compressible Swept Convex-Corner Flow Using Pressure-Sensitive Paint
Authors: Chung Kung-Ming , Huang Yi-Xuan
Published: April 2021

This study used pressure-sensitive paint (PSP) and determined the surface pressure distributions for a compressible swept convex-corner flow. The freestream Mach numbers were 0.64 and 0.83. The convex-corner angle and swept angle were, respectively, 10–17° and 5–15°. Expansion and compression near the corner apex were clearly visualized. For the test case of shock-induced boundary layer separation, there were greater spanwise pressure gradient and curved shocks. The acquired PSP data agree with the experimental data measured using the Kulite pressure transducers for a subsonic expansion flow. For a transonic expansion flow, the discrepancy was significant. The assumption of a constant recovery factor is not valid in the separation region, and temperature correction for PSP measurements is required.
Responses of Soil Abiotic Properties and Microbial Community Structure to 25-Year Cucumber Monoculture in Commercial Greenhouses
Authors: Gao Yu-Han , Lu Xiao-Hong , Guo Rong-Jun , Hao Jian-Jun , Miao Zuo-Qing , Yang Li , Li Shi-Dong
Published: April 2021

Cucumber monoculture could cause soil salinization and acidification, soilborne diseases, and eventually yield loss. However, after a 25-year monoculture in Wafangdian county, Liaoning province, China, cucumber yields have remained satisfactory. In the present study, we investigated how the soil abiotic and biotic properties, in addition to soil rhizosphere microbial community structure in the greenhouses, could still sustain plant growth after such long-term monoculture production. Soil organic carbon, total nitrogen, and available nitrogen were accumulated significantly after monoculture. Such increasing soil fertility was accompanied by increased soil microbial abundance, which reshaped soil microbial community structure. Both bacterial and fungal diversity, including observed and estimated richness, Heip evenness, and Shannon diversity, decreased significantly, while the monoculture period had a greater influence on fungal diversity than on bacterial diversity. Although the high accumulation of soil nutrients increased soil salinity and acidity, the abundance of potential plant pathogenic fungi did not show an obvious increase. These results indicated that maintaining soil abiotic and biotic properties using organic fertilizers and balanced chemical fertilizers, especially improving potassium fertilizer application, could be useful measurements for the sustainable development of greenhouse vegetable production. In addition, appropriate management strategies should be considered to reduce the potential risk of soil salinization.
Is Futurization the Culprit for the Violent Fluctuation in China’s Apple Spot Price?
Authors: Xie Lin , Liao Jiahua , Chen Haiting , Yan Xuefei , Hu Xinyan
Published: April 2021

China aims to utilize the futures market to stabilize agricultural product price fluctuation by quantifying the effects of risk transfer and price discovery. However, the role of futurization has been questioned and even posited as the cause of drastic fluctuations in spot market prices. This research aims to clarify the impact of futurization on the price fluctuation of agricultural products and to provide policy reference for the development of the agricultural futures market through the research. Here, we examine the spot price data for apples and use Interrupted time-series analysis (ITSA) and GARCH models to estimate the impact of apple futures on the volatility of spot prices. Our findings demonstrate that the launch of China’s apple futures did not increase the volatility of apple spot prices; that is, futurization was not the cause of skyrocketing apple spot prices. In the long term, our results suggest that futures will help reduce the volatility of apple spot prices and that the introduction of futures will ultimately reduce the price volatility of agricultural products.
Cover Cropping Impacts Soil Microbial Communities and Functions in Mango Orchards
Authors: Wei Zhiyuan , Zeng Quanchao , Tan Wenfeng
Published: April 2021

Soil microbes play critical roles in nutrient cycling, net primary production, food safety, and climate change in terrestrial ecosystems, yet their responses to cover cropping in agroforestry ecosystems remain unknown. Here, we conducted a field experiment to assess how changes in cover cropping with sown grass strips affect the fruit yields and quality, community composition, and diversity of soil microbial taxa in a mango orchard. The results showed that two-year cover cropping increased mango fruit yields and the contents of soluble solids. Cover cropping enhanced soil fungal diversity rather than soil bacterial diversity. Although cover cropping had no significant effects on soil bacterial diversity, it significantly influenced soil bacterial community compositions. These variations in the structures of soil fungal and bacterial communities were largely driven by soil nitrogen, which positively or negatively affected the relative abundance of both bacterial and fungal taxa. Cover cropping also altered fungal guilds, which enhanced the proportion of pathotrophic fungi and decreased saprotrophic fungi. The increase in fungal diversity and alterations in fungal guilds might be the main factors to consider for increasing mango fruit yields and quality. Our results indicate that cover cropping affects mango fruit yields and quality via alterations in soil fungal diversity, which bridges a critical gap in our understanding of the linkages between soil biodiversity and fruit quality in response to cover cropping in orchard ecosystems.
Spatial and Temporal Variation in the Aphid–Parasitoid Interaction under Different Climates
Authors: Sepúlveda Daniela A. , Barrueto Gonzalo , Correa Margarita C. G. , Castañeda Luis E. , Figueroa Christian C.
Published: April 2021

Global warming will increase pest insect population sizes and diminish the effectiveness of biological control. This biological control failure scenario appears to be of particular concern for areas with a significant increase in maximum temperatures, such as the increase experienced in the Central Valley of Chile over the last 40 years. We assessed the impact of different climatic zones and maximum temperatures along the coast and the Chilean Central Valley on the grain aphid (Sitobion avenae) density, parasitism rate, and facultative endosymbionts in wheat fields during the growing season in the springs of 2017 and 2018. A significant effect on aphid density due to zones and maximum temperatures was detected; however, this depended on the zone and year analyzed. Changes between zones and seasons were observed for parasitism rates, while maximum temperatures only significantly affected the parasitism rate in 2017. The main parasitoid wasp found was Aphidius ervi in both zones and seasons. Regiella insecticola infected 95% of the samples in both zones, although it does not seem to have a protective role at the field level. Our findings suggest that, at present, global warming does not significantly affect the grain aphid outbreaks and their biological control in Chile. However, this study points out the importance of pre-emptive monitoring to detect aphids and the synchrony loss of their parasitoid wasps.
Effects on Crop Development, Yields and Chemical Composition of Celeriac (Apium graveolens L. var. rapaceum) Cultivated Underneath an Agrivoltaic System
Authors: Weselek Axel , Bauerle Andrea , Zikeli Sabine , Lewandowski Iris , Högy Petra
Published: April 2021

Agrivoltaic (AV) systems increase land productivity through the combined production of renewable energy and food. Although several studies have addressed their impact on crop production, many aspects remain unexplored. The objective of this study was to determine the effects of AV on the cultivation of celeriac, a common root vegetable in Central Europe. Celeriac was cultivated in 2017 and 2018 as part of an organically managed on-farm experiment, both underneath an AV system and in full-sun conditions. Under AV, photosynthetic active radiation was reduced by about 30%. Monitoring of crop development showed that in both years, plant height increased significantly under AV. Fresh bulb yield decreased by about 19% in 2017 and increased by about 12% in 2018 in AV, but the changes were not significant. Aboveground biomass increased in both years under AV, but only increased significantly in 2018. As aboveground biomass is a determinant of root biomass at harvest in root vegetables, bulb yields may be further increased by a prolonged vegetation period under AV. Compound analysis of celeriac bulbs did not show any clear effects from treatment. As harvestable yields were not significantly reduced, we concluded that celeriac can be considered a suitable crop for cultivation under AV.
Seed Morphology in Key Spanish Grapevine Cultivars
Authors: Cervantes Emilio , Martín-Gómez José Javier , Espinosa-Roldán Francisco Emmanuel , Muñoz-Organero Gregorio , Tocino Ángel , Cabello-Sáenz de Santamaría Félix
Published: April 2021

Ampelography, the botanical discipline dedicated to the identification and classification of grapevine cultivars, was grounded on the description of morphological characters and more recently is based on the application of DNA polymorphisms. New methods of image analysis may help to optimize morphological approaches in ampelography. The objective of this study was the classification of representative cultivars of Vitis vinifera conserved in the Spanish collection of IMIDRA according to seed shape. Thirty eight cultivars representing the diversity of this collection were analyzed. A consensus seed silhouette was defined for each cultivar representing the geometric figure that better adjusted to their seed shape. All the cultivars tested were classified in ten morphological groups, each corresponding to a new model. The models are geometric figures defined by equations and similarity to each model is evaluated by quantification of percent of the area shared by the two figures, the seed and the model (J index). The comparison of seed images with geometric models is a rapid and convenient method to classify cultivars. A large proportion of the collection may be classified according to the new models described and the method permits to find new models according to seed shape in other cultivars.
Remote and Proximal Sensing-Derived Spectral Indices and Biophysical Variables for Spatial Variation Determination in Vineyards
Authors: Darra Nicoleta , Psomiadis Emmanouil , Kasimati Aikaterini , Anastasiou Achilleas , Anastasiou Evangelos , Fountas Spyros
Published: April 2021

Remote-sensing measurements are crucial for smart-farming applications, crop monitoring, and yield forecasting, especially in fields characterized by high heterogeneity. Therefore, in this study, Precision Viticulture (PV) methods using proximal- and remote-sensing technologies were exploited and compared in a table grape vineyard to monitor and evaluate the spatial variation of selected vegetation indices and biophysical variables throughout selected phenological stages (multi-seasonal data), from veraison to harvest. The Normalized Difference Vegetation Index and the Normalized Difference Red-Edge Index were calculated by utilizing satellite imagery (Sentinel-2) and proximal sensing (active crop canopy sensor Crop Circle ACS-470) to assess the correlation between the outputs of the different sensing methods. Moreover, numerous vegetation indices and vegetation biophysical variables (VBVs), such as the Modified Soil Adjusted Vegetation Index, the Normalized Difference Water Index, the Fraction of Vegetation Cover, and the Fraction of Absorbed Photosynthetically Active Radiation, were calculated, using the satellite data. The vegetation indices analysis revealed different degrees of correlation when using diverse sensing methods, various measurement dates, and different parts of the cultivation. The results revealed the usefulness of proximal- and remote-sensing-derived vegetation indices and variables and especially of Normalized Difference Vegetation Index and Fraction of Absorbed Photosynthetically Active Radiation in the monitoring of vineyard condition and yield examining, since they were demonstrated to have a very high degree of correlation (coefficient of determination was 0.87). The adequate correlation of the vegetation indices with the yield during the latter part of the veraison stage provides valuable information for the future estimation of production in broader areas.
Herbaceous Field Crops’ Cultivation
Authors: Lombardo Sara , Mauromicale Giovanni
Published: April 2021

Herbaceous field crops include several hundred plant species spread worldwide for different end-uses, from food to non-food applications [...]
Correlation Analysis of Compounds in Essential Oil of Amomum tsaoko Seed and Fruit Morphological Characteristics, Geographical Conditions, Locality of Growth
Authors: Li Guodong , Lu Qinwei , Wang Jingjian , Hu Qingyu , Liu Pinghui , Yang Yaowen , Li Yongkun , Tang Huiru , Xie Hui
Published: April 2021

Amomum tsaoko is a perennial herb belonging to Zingiberaceae. Its dried ripe fruit is an important food additive, spice and materia medicai in Southeast Asia. For hundreds of years of cultivation, morphological variations have existed. The essential oil is one of the major active products of the A. tsaoko fruit and seed. In this study, we collected 12 populations in Yunnan province, and the correlation analysis of compounds was focused on the essential oil of A. tsaoko seed and its fruit morphological characteristics, geographical conditions, and locality of growth. The results showed that the difference in morphological characteristics between populations is greater than the difference within the population. High altitude areas are beneficial for biomass accumulation. Another interesting finding is that selecting specific functional or odor type materials could reference the morphologies of A. tsaoko fruit and seed. Furthermore, the qualitative and quantitative analysis of compounds in essential oil could be used to distinguish the producing area of the A. tsaoko fruit. These results are crucial in realizing the determination of botanical origin and evaluating the quality of A. tsaoko fruit. Meanwhile, it makes clear that various other studies on this plant deserve more attention.
The Protective Biochemical Properties of Arbuscular Mycorrhiza Extraradical Mycelium in Acidic Soils Are Maintained throughout the Mediterranean Summer Conditions
Authors: Faria Jorge M. S. , Teixeira Dora Martins , Pinto Ana Paula , Brito Isabel , Barrulas Pedro , Carvalho Mário
Published: April 2021

In acidic soils with manganese (Mn) toxicity, arbuscular mycorrhizal fungi (AMF) can improve plant host growth by enhancing nutrition and protecting against environmental stress. The intact extraradical mycelium (ERM) of AMF is able to survive Mediterranean summer conditions and provide an earlier colonization of winter crops. This study evaluated if summer season conditions hindered the beneficial effects of wheat colonization by the intact ERM associated with a native plant, in acidic soil. Wheat was grown in soil with intact or disrupted ERM associated with Ornithopus compressus (ORN), developed for 7 or 24 weeks, to simulate ERM summer survival. The activity of antioxidant enzymes was determined, and the quantitative analysis of Mn and macronutrients was performed by inductively coupled plasma mass spectrometry (ICP-MS), in wheat shoots and respective subcellular fractions. Wheat colonization by intact ERM decreased shoot Mn concentration but increased the proportion of Mn in the apoplast. Overall, antioxidant enzymatic activity decreased but the proportion of Mn-superoxide dismutase activity over the remaining isoforms increased, suggesting its important role in the AMF-mediated mitigation of Mn toxicity. Summer conditions did not substantially reduce the benefits provided by ORN ERM. A no-till strategy allied to the development of native microbiota can contribute to the sustainable optimization of acidic soil use.
Winter Wheat Resistant to Increases in Rain and Snow Intensity in a Semi-Arid System
Authors: Holdrege Martin C. , Beard Karen H. , Kulmatiski Andrew
Published: April 2021

As the atmosphere warms, precipitation events have been predicted and observed to become fewer and larger. Changes in precipitation patterns can have large effects on dryland agricultural production, but experimental tests on the effects of changing precipitation intensity are limited. Over 3 years, we tested the effects of increased precipitation intensity on winter wheat (Triticum aestivum L.; Promontory variety) in a temperate dryland agricultural system that was on a rotation of crop and fallow years. We used 11 (2.1 × 2.5 m) shelters to collect and redeposit rain and snow as larger, more intense events. Total precipitation was the same in all plots, but event sizes in each plot varied from 1 to 18 mm. Treatments increased soil water availability, but winter wheat biomass and grain yield did not differ among treatments. Similarly, other measured plant growth responses, including vegetation greenness, leaf area index, canopy temperature, photochemical efficiency, root area, and new root growth, did not differ among treatments. Results indicate that at least in the semiarid climate and silt loam soils studied here, anticipated increases in precipitation intensity are unlikely to affect winter wheat production negatively. Further, increased precipitation intensity may mitigate water stress caused by increasing temperatures and encourage the use of wheat varieties that utilize deeper, later season soil water.
The Effect of Different Feeding Systems on Salivary Cortisol Levels during Gestation in Sows on Herd Level
Authors: Bahnsen Ida , Riddersholm Kristina V. , de Knegt Leonardo V. , Bruun Thomas S. , Amdi Charlotte
Published: April 2021

The aim of this study was to investigate herd cortisol levels as an indicator of stress during gestation in three different feeding systems. Twelve commercial Danish herds with 800 to 3050 sows were included, with either free-access feeding stall (Stall), floor feeding (Floor), or electronic sow feeding (ESF; n = 4 herds per system). Saliva samples were collected from 30 sows/herd in the gestation unit for cortisol analysis with an average of 67.2 gestation days for ESF, 72.4 days for Floor, and 68.6 days for Stall. Data on piglet birth weight (PBW) and the percentage of intrauterine growth restricted (IUGR) piglets from 452 litters (9652 piglets, 8677 liveborn) from all 12 herds were obtained on the saliva collection days. The cortisol levels in saliva increased throughout gestation (p < 0.01), and lower concentrations were observed among sows belonging to Stall (4.80 nmol/L), compared to Floor (7.03 nmol/L) and ESF (7.87 nmol/L), and that difference was significant as an independent effect in the case of ESF (p < 0.01). There was no difference between Floor and ESF or Stall and Floor (p > 0.05). An interaction was observed between parity and feeding system, with parities 4–5 in ESF herds having lower levels than other parities within the ESF system (p = 0.02).
Pain at the Slaughterhouse in Ruminants with a Focus on the Neurobiology of Sensitisation
Authors: Mota-Rojas Daniel , Napolitano Fabio , Strappini Ana , Orihuela Agustín , Ghezzi Marcelo Daniel , Hernández-Ávalos Ismael , Mora-Medina Patricia , Whittaker Alexandra L.
Published: April 2021

We pose, based on a neurobiological examination, that events that occur around the time of slaughter have the potential to intensify the pain response, through the processes of sensitisation and enhanced transmission. Sensitisation, or an enhanced response to painful stimuli, is a well-discussed phenomenon in the human medical literature, which can arise from previous injury to an area, inflammatory reactions, or previous overstimulation of the stress axes. A number of events that occur prior to arrival at, or in the slaughterhouse, may lead to presence of these factors. This includes previous on-farm pathology, injuries arising from transport and handling and lack of habituation to humans. Whilst there is limited evidence of a direct effect of these on the processes of sensitisation in animals at slaughter, by analogy with the human neurobiology literature the connection seems plausible. In this review a neurobiological approach is taken to discuss this hypothesis in the light of basic science, and extrapolations from existing literature on the slaughter of ruminants. To confirm the postulated link between events at slaughter, and processes of hypersensitisation, further dedicated study is required.
Effect of Broilers Chicken Diet Supplementation with Natural and Acidified Humic Substances on Quality of Produced Breast Meat
Authors: Hudák Marek , Semjon Boris , Marcinčáková Dana , Bujňák Lukáš , Naď Pavel , Koréneková Beáta , Nagy Jozef , Bartkovský Martin , Marcinčák Slavomír
Published: April 2021

This study was conducted to examine the effect of two humic substances (HS) supplemented in broilers’ diet on the breast meat quality of broiler chickens. In this experiment, 120 pieces of one-day-old COBB500 broiler chickens were used. Broilers were divided into three groups, each containing 40 birds with three replications (13, 13, and 14 per one pen). Fattening lasted 38 days. The first experimental diet was supplemented with 0.7% of HS (HS0.7) and the second was enriched with 0.7% of acidified HS (HSA0.7). The control group of broilers (C) was fed a basal diet without the addition of any supplements. HS0.7 samples had the highest total protein content and the lowest content of fat (p < 0.01). The effects of broiler diet and storage had a significant impact on the pH of breast samples, p < 0.001 and p < 0.05, respectively. Supplementation of HS in broiler diet positively affected the lipid oxidation of breast meat samples, as indicated by the lower malondialdehyde content (p < 0.05). HSA0.7 samples had significantly better juiciness after the storage period (p < 0.001). The quality of meat produced with supplementation of HS in broilers’ feed can be considered as valuable in human nutrition due to improved protein and fat content with decreased lipid oxidation.
The Physiological Roles of Vitamin E and Hypovitaminosis E in the Transition Period of High-Yielding Dairy Cows
Authors: Haga Satoshi , Ishizaki Hiroshi , Roh Sanggun
Published: April 2021

Levels of alpha-tocopherol (α-Toc) decline gradually in blood throughout prepartum, reaching lowest levels (hypovitaminosis E) around calving. Despite numerous reports about the disease risk in hypovitaminosis E and the effect of α-Toc supplementation on the health of transition dairy cows, its risk and supplemental effects are controversial. Here, we present some novel data about the disease risk of hypovitaminosis E and the effects of α-Toc supplementation in transition dairy cows. These data strongly demonstrate that hypovitaminosis E is a risk factor for the occurrence of peripartum disease. Furthermore, a study on the effectiveness of using serum vitamin levels as biomarkers to predict disease in dairy cows was reported, and a rapid field test for measuring vitamin levels was developed. By contrast, evidence for how hypovitaminosis E occurred during the transition period was scarce until the 2010s. Pioneering studies conducted with humans and rodents have identified and characterised some α-Toc-related proteins, molecular players involved in α-Toc regulation followed by a study in ruminants from the 2010s. Based on recent literature, the six physiological factors: (1) the decline in α-Toc intake from the close-up period; (2) changes in the digestive and absorptive functions of α-Toc; (3) the decline in plasma high-density lipoprotein as an α-Toc carrier; (4) increasing oxidative stress and consumption of α-Toc; (5) decreasing hepatic α-Toc transfer to circulation; and (6) increasing mammary α-Toc transfer from blood to colostrum, may be involved in α-Toc deficiency during the transition period. However, the mechanisms and pathways are poorly understood, and further studies are needed to understand the physiological role of α-Toc-related molecules in cattle. Understanding the molecular mechanisms underlying hypovitaminosis E will contribute to the prevention of peripartum disease and high performance in dairy cows.
Performance Evaluation of Two Slow-Medium Growing Chicken Strains Maintained under Organic Production System during Different Seasons
Authors: Sarmiento-García Ainhoa , Revilla Isabel , Abecia José-Alfonso , Palacios Carlos
Published: April 2021

A total of 160 1-day-old medium-growing male chicks (Gallus gallus domesticus) were raised for 120 days in a certified organic farming system. A total of two strains were studied (Coloryield, CY; RedBro, RB). Overall, two weather periods were considered based on the outdoor temperature, being S1 colder than S2. In total, 40 chicks per strain were assigned to each period (n = 80). Chickens were fed ad libitum with the same organic feeds. In the first month, chickens were kept indoors and, from day 30, they had access to the pasture. Slaughter live weight (LW), average daily gains, (ADG), the feed conversion ratio (FCR), and mortality rates did not differ between the two strains. LW was (p < 0.05) higher in the S1 and a trend (p = 0.084) was observed for ADG, which was higher in S1. No differences were found for feed intake, FCR, and mortality rates between weather periods. There were no differences for coefficient of variation (CV) between the strains studied, nevertheless, CV for LW in S2 was increased. Differences in the productive performance between these strains raised in organic production systems were slight. However, chickens raised in S1 had a better performance. It would be preferable to raise chickens in these weather conditions whenever possible.
EZ-DripLoss Assessment in Chicken Breast Meat Using Different Sample Areas, Fiber Orientation, and Measurement Intervals
Authors: Kaić Ana , Janječić Zlatko , Žanetić Andrija , Kelava Ugarković Nikolina , Potočnik Klemen
Published: April 2021

Although the EZ-DripLoss method has been performed in numerous studies, there is a deficiency in our knowledge of the EZ-DripLoss method’s suitability for poultry meat analysis. This study aimed to research the effect of different sample areas (10, 20, and 30 mm; n = 240), and fiber orientations (vertical vs. horizontal) on the EZ-DripLoss in chicken breast meat measured across a period of five days. The influence of sample area on the EZ-DripLoss of chicken breast meat with respect to the fiber orientation and across the five-day measurement interval was significant between 10 and 30 mm samples, and between 20 and 30 mm samples (p < 0.001). The estimated regression coefficient showed that EZ-DripLoss for the samples with horizontal and vertical fiber direction of 10 and 20 mm, significantly increased by 0.04% per hour, while for the samples with vertical fiber direction whose diameter was 30 mm, it significantly increased by 0.06% per hour, and for the horizontal fiber direction, it significantly increased by 0.07% per hour. The samples with vertical fiber orientation had 0.50% greater EZ-DripLoss compared to the samples with horizontal fiber orientation. EZ-DripLoss evaluation in chicken breast should be performed with a sample core diameter of 20 mm, a vertical fiber orientation, and over the course of longer measurement intervals.
Comparative Phenotypic and Functional Analyses of the Effects of IL-10 or TGF-β on Porcine Macrophages
Authors: Carta Tania , Razzuoli Elisabetta , Fruscione Floriana , Zinellu Susanna , Meloni Dionigia , Anfossi Antonio , Chessa Bernardo , Dei Giudici Silvia , Graham Simon P. , Oggiano Annalisa , Franzoni Giulia
Published: April 2021

Macrophages are phagocytic cells involved in maintaining tissue homeostasis and defense against pathogens. Macrophages may be polarized into different functionally specialized subsets. M2c macrophages arise following stimulation with IL-10 or TGF-β and mediate anti-inflammatory and tissue repair functions. M2c macrophages remain poorly characterized in the pig, thus we investigated the impact of these regulatory cytokines on porcine monocyte-derived macrophages (moMΦ). The phenotype and functionality of these cells was characterized though confocal microscopy, flow cytometry, ELISA, and RT-qPCR. Both cytokines induced CD14 and MHC II DR down-regulation and reduced IL-6, TNF-α, and CD14 expression, suggestive of an anti-inflammatory phenotype. Interestingly, neither IL-10 or TGF-β were able to trigger IL-10 induction or release by moMΦ. Differences between these cytokines were observed: stimulation with IL-10, but not TGF-β, induced up-regulation of both CD16 and CD163 on moMΦ. In addition, IL-10 down-regulated expression of IL-1β and IL-12p40 4h post-stimulation and induced a stronger impairment of moMΦ ability to respond to either TLR2 or TLR4 agonists. Overall, our results provide an overview of porcine macrophage polarization by two immunosuppressive cytokines, revealing differences between IL-10 and TGF-β, and reporting some peculiarity of swine, which should be considered in translational studies.
Prevalence and Characterization of Quinolone-Resistance Determinants in Escherichia coli Isolated from Food-Producing Animals and Animal-Derived Food in the Philippines
Authors: Belotindos Lawrence , Villanueva Marvin , Miguel Joel , Bwalya Precious , Harada Tetsuya , Kawahara Ryuji , Nakajima Chie , Mingala Claro , Suzuki Yasuhiko
Published: April 2021

Antimicrobial resistance to quinolones, which constitutes a threat to public health, has been increasing worldwide. In this study, we investigated the prevalence of quinolone-resistant determinants in Escherichia coli not susceptible to quinolones and isolated from food-producing animals and food derived from them, in the Philippines. A total of 791 E. coli strains were isolated in 56.4% of 601 beef, chicken, pork, egg, and milk samples, as well as environmental, cloacal, and rectal swab-collected samples from supermarkets, open markets, abattoirs, and poultry, swine, and buffalo farms. Using the disc diffusion method, it was determined that 78.6% and 55.4% of the isolates were resistant to at least one antimicrobial and multiple drugs, respectively. In 141 isolates not susceptible to quinolones, 115 (81.6%) harbored quinolone-resistant determinants and had mutations predominantly in the quinolone-resistance determining regions (QRDRs) of gyrA and parC. Plasmid-mediated, quinolone resistance (PMQR) and Qnr family (qnrA1, qnrB4, and qnrS1) genes were detected in all isolates. Forty-eight sequence types were identified in isolates harboring mutations in QRDR and/or PMQR genes by multilocus sequence typing analysis. Moreover, 26 isolates harboring mutations in QRDR and/or PMQR genes belonged mostly to phylogroup B1 and Enteroaggregative E. coli. In conclusion, a high prevalence of E. coli was found in food-producing animals and products derived from them, which could potentially spread high-risk clones harboring quinolone-resistance determinants.
Essential Oils: A Natural Weapon against Antibiotic-Resistant Bacteria Responsible for Nosocomial Infections
Authors: Iseppi Ramona , Mariani Martina , Condò Carla , Sabia Carla , Messi Patrizia
Published: April 2021

The emergence of antibiotic-resistant bacteria has become a major concern worldwide. This trend indicates the need for alternative agents to antibiotics, such as natural compounds of plant origin. Using agar disc diffusion and minimum inhibitory concentration (MIC) assays, we investigated the antimicrobial activity of Citrus aurantium (AEO), Citrus x limon (LEO), Eucalyptus globulus (EEO), Melaleuca alternifolia (TTO), and Cupressus sempervirens (CEO) essential oils (EOs) against three representatives of antibiotic-resistant pathogens and respective biofilms: vancomycin-resistant enterococci (VRE), methicillin-resistant Staphylococcus aureus (MRSA), and extended-spectrum β-lactamase (ESBL)-producing Escherichia coli. Using the checkerboard method, the efficacy of the EOs alone, in an association with each other, or in combination with the reference antibiotics was quantified by calculating fractional inhibitory concentrations (FICs). All the EOs displayed antibacterial activity against all strains to different extents, and TTO was the most effective. The results of the EO–EO associations and EO–antibiotic combinations clearly showed a synergistic outcome in most tests. Lastly, the effectiveness of EOs both alone and in association or combination against biofilm formed by the antibiotic-resistant strains was comparable to, and sometimes better than, that of the reference antibiotics. In conclusion, the combination of EOs and antibiotics represents a promising therapeutic strategy against antibiotic-resistant bacteria, even protected inside biofilms, which can allow decreasing the concentrations of antibiotics used.