bluuebunny/crossref_metadata_2025 huggingface dataset:
Trends in Hospital Admissions for Pertussis Infection: A Nationwide Retrospective Observational Study in Italy, 2002–2016
Authors: Fiasca Fabiana , Gabutti Giovanni , Mattei Antonella
Published: November 2019

Background: Pertussis is a highly contagious infectious disease which continues to be an important public-health issue despite the high immunization coverage rates achieved. However, evidence of increased circulation of pertussis among adolescents and adults due to waning immunity and atypical clinical manifestations seem to be the main reasons for its resurgence. The aim of this study was the analysis of the epidemiological trend for pertussis-related hospitalizations in Italy, in relation with vaccination coverage and information from laboratory confirmed cases of pertussis. Methods: A retrospective observational study investigating hospitalizations for pertussis from 2002 to 2016 in Italy was conducted. Frequencies and rates of hospitalization were analyzed and hospitalization data were compared with a series of already published laboratory confirmed data. Results: This study highlighted a rising trend for pertussis hospitalizations in Italy since 2008. Infants aged <1 year showed the highest frequencies (63.39%) and average rates (74.60 × 100000 infants) of hospitalization despite an extremely high vaccination coverage (95.89%). An increasing trend of hospitalization frequency emerged for the age group with levels of IgG antibodies to pertussis toxin compatible with pertussis infection within the last year (20–29 years old age group). Conclusions: The rising trend for pertussis hospitalizations and the greater involvement of infants aged <1 year require an integrated approach, including the implementation of booster doses administration in adolescence and adulthood, the vaccination of pregnant women and the cocoon strategy.
Prolongation of Tick-Borne Encephalitis Cycles in Warmer Climatic Conditions
Authors: Zeman Petr
Published: November 2019

Tick-borne encephalitis exhibits profound inter-annual fluctuations in incidence. Previous studies showed that three-fifths of the variation can be explained in terms of four superimposed oscillations: a quasi-biennial, triennial, pentennial, and a decadal cycle. This study was conducted to determine how these cycles could be influenced by climate change. Epidemiological data, spanning from the 1970s to the present, and originating from six regions/countries bridging Scandinavia and the Mediterranean, represented a temporal/latitudinal gradient. Spectral analysis of time series was used to determine variation in the cycles’ length/amplitude with respect to these gradients. The analysis showed that—whereas the lengths of the shorter cycles do not vary substantially—cycles in the decadal band tend to be longer southwards. When comparing the disease’s oscillations before- and after the mid-1990s, a shift towards longer oscillations was detected in the pentennial–decadal band, but not in the biennial– triennial band. Simultaneously, oscillations in the latter band increased in intensity whereas the decadal oscillations weakened. In summary, the rhythm of the cycles has been altered by climate change. Lengthened cycles may be explained by prolonged survival of some animal hosts, and consequently greater inertia in herd immunity changes, slowing down a feedback loop between the herd immunity and amount of virus circulating in nature.
Indoor Location Prediction Method for Shopping Malls Based on Location Sequence Similarity
Authors: Wang Peixiao , Wu Sheng , Zhang Hengcai , Lu Feng
Published: November 2019

Fast and accurate indoor location prediction plays an important part in indoor location services. This work proposes an indoor location prediction framework named Indoor-WhereNext. First, a novel algorithm, “indoor spatiotemporal density-based spatial clustering of applications with noise” (Indoor-STDBSCAN), is proposed to detect the stay points in an indoor trajectory and convert them into a location sequence. Then, a spatial-semantic similarity (SSS) method for measuring the similarity between location sequences is defined. SSS comprehensively considers the spatial and semantic similarities between location sequences. Finally, a clustering algorithm is used to obtain similarity user groups based on SSS. These groups are used to train different prediction models to achieve improved results. Extensive experiments were conducted using real indoor Wi-Fi positioning datasets collected in a shopping mall. The results show that the Indoor-WhereNext model markedly outperforms the three existing baseline methods in terms of prediction accuracy and precision.
A Comparative Analysis of Edwardsiella tarda-Induced Transcriptome Profiles in RAW264.7 Cells Reveals New Insights into the Strategy of Bacterial Immune Evasion
Authors: Li Huili , Sun Boguang , Ning Xianhui , Jiang Shuai , Sun Li
Published: November 2019

Edwardsiella tarda is a Gram-negative bacterial pathogen with a broad host range, including fish, reptiles, and mammals. One prominent virulence feature of E. tarda is its ability to survive and replicate in host phagocytes, but the relevant molecular mechanism is largely unknown. In this study, we examined the transcriptome profiles of RAW264.7 cells, a murine macrophage cell line, infected with live E. tarda or stimulated with dead E. tarda for 4 h and 8 h. Eighteen libraries were constructed, and an average of 69 million clean reads per library were obtained, with ~81.63% of the reads being successfully mapped to the reference genome. In total, 208 and 232 differentially expressed genes (DEGs) were identified between live and dead E. tarda-treated cells at 4 h and 8 h post-infection, respectively. The DEGs were markedly enriched in the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways associated with immunity. Live E. tarda differed strikingly from dead E. tarda in the regulation of immune related genes. Compared with dead E. tarda-treated cells, live E. tarda-treated cells exhibited marked and significant suppression in the induction of a large amount of immune genes, including RIG-I-like receptors, cytokines, and interferon-related genes. Furthermore, some of the immune genes highly regulated by live E. tarda formed complicated interaction networks with each other. Together, the results of this study revealed a transcriptome profile specifically induced by the active virulence elements of live E. tarda during the infection process, thus adding new insights into the intracellular infection mechanism of E. tarda. This study also provided a valuable set of target genes for further study of the immune evasion strategy of E. tarda.
An Association of Serotonin with Pain Disorders and Its Modulation by Estrogens
Authors: Paredes Stephania , Cantillo Santiago , Candido Kenneth D. , Knezevic Nebojsa Nick
Published: November 2019

Ovarian hormones play an important role in pain perception, and are responsible, at least in part, for the pain threshold differences between the sexes. Modulation of pain and its perception are mediated by neurochemical changes in several pathways, affecting both the central and peripheral nervous systems. One of the most studied neurotransmitters related to pain disorders is serotonin. Estrogen can modify serotonin synthesis and metabolism, promoting a general increase in its tonic effects. Studies evaluating the relationship between serotonin and disorders such as irritable bowel syndrome, fibromyalgia, migraine, and other types of headache suggest a clear impact of this neurotransmitter, thereby increasing the interest in serotonin as a possible future therapeutic target. This literature review describes the importance of substances such as serotonin and ovarian hormones in pain perception and illustrates the relationship between those two, and their direct influence on the presentation of the aforementioned pain-related conditions. Additionally, we review the pathways and receptors implicated in each disorder. Finally, the objective was to stimulate future pharmacological research to experimentally evaluate the potential of serotonin modulators and ovarian hormones as therapeutic agents to regulate pain in specific subpopulations.
Adiponectin Reverses the Hypothalamic Microglial Inflammation during Short-Term Exposure to Fat-Rich Diet
Authors: Lee Hannah , Tu Thai , Park Byong , Yang Sunggu , Kim Jae
Published: November 2019

Adiponectin, an adipokine derived from the adipose tissue, manifests anti-inflammatory effects in the metabolically active organs and is, therefore, beneficial in various metabolic diseases associated with inflammation. However, the role of adiponectin in alleviating the hypothalamic inflammation connected to the pathogenesis of obesity has not yet been clearly interrogated. Here, we identified that the systemic administration of adiponectin suppresses the activation of microglia and thereby reverses the hypothalamic inflammation during short-term exposure to a high-fat diet. Additionally, we show that adiponectin induces anti-inflammatory effects in the microglial cell line subjected to an exogenous treatment with a saturated free fatty acid. In conclusion, the current study suggests that adiponectin suppresses the saturated free fatty acid-triggered the hypothalamic inflammation by modulating the microglial activation and thus maintains energy homeostasis.
SiMYB3 in Foxtail Millet (Setaria italica) Confers Tolerance to Low-Nitrogen Stress by Regulating Root Growth in Transgenic Plants
Authors: Ge Linhao , Dou Yining , Li Maomao , Qu Pengju , He Zhang , Liu Y , Xu Zhaoshi , Chen Jun , Chen Ming , Ma Youzhi
Published: November 2019

Foxtail millet (Setaria italica), which originated in China, has a strong tolerance to low nutrition stresses. However, the mechanism of foxtail millet tolerance to low-nitrogen stress is still unknown. In this study, the transcriptome of foxtail millet under low-nitrogen stress was systematically analyzed. Expression of 1891 genes was altered, including 1318 up-regulated genes and 573 down-regulated genes. KEGG (Kyoto Encyclopedia of Genes and Genomes) analysis revealed that 3% of these genes were involved in membrane transport and 5% were involved in redox processes. There were 74 total transcription factor (TF) genes in the DEGs (differentially expressed genes), and MYB-like transcription factors accounted for one-third (25) of the TF genes. We systematically analyzed the characteristics, expression patterns, chromosome locations, and protein structures of 25 MYB-like genes. The analysis of gene function showed that Arabidopsis and rice overexpressing SiMYB3 had better root development than WT under low-nitrogen stress. Moreover, EMSA results showed that SiMYB3 protein could specifically bind MYB elements in the promoter region of TAR2, an auxin synthesis related gene and MYB3-TAR2 regulate pair conserved in rice and foxtail millet. These results suggested that SiMYB3 can regulate root development by regulating plant root auxin synthesis under low-nitrogen conditions.
The Effects of Meldonium on the Renal Acute Ischemia/Reperfusion Injury in Rats
Authors: Đurašević Siniša , Stojković Maja , Bogdanović Ljiljana , Pavlović Slađan , Borković-Mitić Slavica , Grigorov Ilijana , Bogojević Desanka , Jasnić Nebojša , Tosti Tomislav , Đurović Saša , Đorđević Jelena , Todorović Zoran
Published: November 2019

Acute renal ischemia/reperfusion (I/R) injury is a clinical condition that is challenging to treat. Meldonium is an anti-ischemic agent that shifts energy production from fatty acid oxidation to less oxygen-consuming glycolysis. Thus, in this study we investigated the effects of a four-week meldonium pre-treatment (300 mg/kg b.m./day) on acute renal I/R in male rats (Wistar strain). Our results showed that meldonium decreased animal body mass gain, food and water intake, and carnitine, glucose, and lactic acid kidney content. In kidneys of animals subjected to I/R, meldonium increased phosphorylation of mitogen-activated protein kinase p38 and protein kinase B, and increased the expression of nuclear factor erythroid 2-related factor 2 and haeme oxygenase 1, causing manganese superoxide dismutase expression and activity to increase, as well as lipid peroxidation, cooper-zinc superoxide dismutase, glutathione peroxidase, and glutathione reductase activities to decrease. By decreasing the kidney Bax/Bcl2 expression ratio and kidney and serum high mobility group box 1 protein content, meldonium reduced apoptotic and necrotic events in I/R, as confirmed by kidney histology. Meldonium increased adrenal noradrenaline content and serum, adrenal, hepatic, and renal ascorbic/dehydroascorbic acid ratio, which caused complex changes in renal lipidomics. Taken together, our results have confirmed that meldonium pre-treatment protects against I/R-induced oxidative stress and apoptosis/necrosis.
Thyroid Hormone Protects from Fasting-Induced Skeletal Muscle Atrophy by Promoting Metabolic Adaptation
Authors: Ucci Sarassunta , Renzini Alessandra , Russi Valentina , Mangialardo Claudia , Cammarata Ilenia , Cavioli Giorgia , Santaguida Maria Giulia , Virili Camilla , Centanni Marco , Adamo Sergio , Moresi Viviana , Verga-Falzacappa Cecilia
Published: November 2019

Thyroid hormones regulate a wide range of cellular responses, via non-genomic and genomic actions, depending on cell-specific thyroid hormone transporters, co-repressors, or co-activators. Skeletal muscle has been identified as a direct target of thyroid hormone T3, where it regulates stem cell proliferation and differentiation, as well as myofiber metabolism. However, the effects of T3 in muscle-wasting conditions have not been yet addressed. Being T3 primarily responsible for the regulation of metabolism, we challenged mice with fasting and found that T3 counteracted starvation-induced muscle atrophy. Interestingly, T3 did not prevent the activation of the main catabolic pathways, i.e., the ubiquitin-proteasome or the autophagy-lysosomal systems, nor did it stimulate de novo muscle synthesis in starved muscles. Transcriptome analyses revealed that T3 mainly affected the metabolic processes in starved muscle. Further analyses of myofiber metabolism revealed that T3 prevented the starvation-mediated metabolic shift, thus preserving skeletal muscle mass. Our study elucidated new T3 functions in regulating skeletal muscle homeostasis and metabolism in pathological conditions, opening to new potential therapeutic approaches for the treatment of skeletal muscle atrophy.
Dense Model for Automatic Image Description Generation with Game Theoretic Optimization
Authors: S R Sreela , Idicula Sumam Mary
Published: November 2019

Due to the rapid growth of deep learning technologies, automatic image description generation is an interesting problem in computer vision and natural language generation. It helps to improve access to photo collections on social media and gives guidance for visually impaired people. Currently, deep neural networks play a vital role in computer vision and natural language processing tasks. The main objective of the work is to generate the grammatically correct description of the image using the semantics of the trained captions. An encoder-decoder framework using the deep neural system is used to implement an image description generation task. The encoder is an image parsing module, and the decoder is a surface realization module. The framework uses Densely connected convolutional neural networks (Densenet) for image encoding and Bidirectional Long Short Term Memory (BLSTM) for language modeling, and the outputs are given to bidirectional LSTM in the caption generator, which is trained to optimize the log-likelihood of the target description of the image. Most of the existing image captioning works use RNN and LSTM for language modeling. RNNs are computationally expensive with limited memory. LSTM checks the inputs in one direction. BLSTM is used in practice, which avoids the problem of RNN and LSTM. In this work, the selection of the best combination of words in caption generation is made using beam search and game theoretic search. The results show the game theoretic search outperforms beam search. The model was evaluated with the standard benchmark dataset Flickr8k. The Bilingual Evaluation Understudy (BLEU) score is taken as the evaluation measure of the system. A new evaluation measure called GCorrectwas used to check the grammatical correctness of the description. The performance of the proposed model achieves greater improvements over previous methods on the Flickr8k dataset. The proposed model produces grammatically correct sentences for images with a GCorrect of 0.040625 and a BLEU score of 69.96%
Low Pre-Treatment Count of Circulating Endothelial Progenitors as a Prognostic Biomarker of the High Risk of Breast Cancer Recurrence
Authors: Rhone Piotr , Bielawski Kornel , Ziołkowska Katarzyna , Rość Danuta , Ruszkowska-Ciastek Barbara
Published: November 2019

Neoangiogenesis is mediated by circulating bone marrow-derived endothelial progenitors (circulating EPCs). The aim of the study was the quantification of circulating EPCs from the peripheral blood mononuclear cells of invasive breast cancer (IBrC) patients by flow cytometry, before and after cancer adjuvant treatment. A total of 88 stage IA-IIB primary IBrC patients were enrolled prospectively. Circulating EPCs with the immune-phenotype CD45−CD34+CD133+CD31+ were assessed. Treatment significantly reduced the number of EPCs/µL in the general IBrC cohort. However, there was a relevant elevation in the number of circulating EPCs after nine months of adjuvant treatment in the group of patients aged ≥ 55 years, of T2 clinical type, with nodal involvement (N1) and Ki67 expression > 15%. Follow-up revealed a significantly higher incidence of disease relapse in breast cancer patients with low pre-treatment circulating EPCs levels compared with those with a high baseline circulating EPCs count. The receiver-operating characteristic curve identified a tumour diameter of 2.1 cm as the best cut-off value to discriminate between disease-relapse subjects and non-relapse disease cases. Our study strongly indicates that, next to tumour diameter and Ki67 expression, circulating bone marrow-derived EPCs may serve as useful markers for predicting therapeutic outcomes as well as a future prognosis.
Rivaroxaban Effects Illustrate the Underestimated Importance of Activated Platelets in Thrombin Generation Assessed by Calibrated Automated Thrombography
Authors: Makhoul Stephanie , Panova-Noeva Marina , Regnault Véronique , Ruf Wolfram , Wenzel Philip , Lagrange Jeremy
Published: November 2019

Background: The direct oral anticoagulant rivaroxaban inhibiting specifically activated factor X (FXa) causes delayed thrombin generation (TG) as measured by calibrated automated thrombography (CAT). The implications of these changes for assessing bleeding or residual prothrombotic risks of patients are unclear in the absence of a better understanding of the underlying mechanism. Methods: We compared platelet rich plasma (PRP) without or with prior collagen-induced platelet aggregation (agPRP) in the CAT assay to better characterize TG in the presence of rivaroxaban. Results: In the presence of rivaroxaban, TG curves in agPRP showed a distinct profile with a rapidly ascending phase followed with a protracted phase. Inhibition of tissue factor pathway inhibitor amplified the first phase of the curve which was also modulated by procoagulant phospholipids. Inhibition of FXIIa-dependent FXI activation revealed that aggregated platelets influenced the first phase by a combination of extrinsic and intrinsic coagulation pathway initiations. Thrombin-dependent amplification of TG (even prior collagen activation) was responsible for the second phase of the TG curve. Conclusions: AgPRP fully includes platelet ability to support TG and reveal distinct TG phases in the presence of direct FXa inhibitors highlighting its potential use in an anticoagulated setting.
Interleukin-18 Is a Prognostic Biomarker Correlated with CD8+ T Cell and Natural Killer Cell Infiltration in Skin Cutaneous Melanoma
Authors: Gil Minchan , Kim Kyung Eun
Published: November 2019

Interleukin-18 (IL-18) is a cytokine that enhances innate and adaptive immune responses. Although there are conflicting reports about the roles of IL-18 in melanoma progression, the clinical relevance of IL-18 expression has not been comprehensively studied. In this study, we investigated IL-18 expression and its correlation with patient survival and immune cell infiltration in melanoma using cancer gene expression data publicly available through various databases. IL18 mRNA expression was found to be significantly lower in melanoma tissues than normal tissues. Kaplan–Meier survival analysis showed that IL18 expression was positively correlated with patient survival. To investigate the possible mechanisms by which IL18 expression increased patient survival, we then assessed the correlation between IL18 expression and immune cell infiltration levels. Infiltration of various immune cells, especially CD8+ T and natural killer (NK) cells, which are cytolytic effector cells, was significantly increased by IL18 expression. Additionally, the expression levels of two cytolytic molecules including perforin and granzyme B were significantly positively correlated with IL18 expression. Collectively, this study provides the first evidence that IL18 expression has prognostic value for melanoma patient survival and is strongly correlated with CD8+ T and NK cell infiltration, suggesting the role of IL-18 as a biomarker for predicting melanoma prognosis.
Physical Health and Psychological Outcomes in Adult Patients with Long-Bone Fracture Non-Unions: Evidence Today
Authors: Johnson Louise , Igoe Emily , Kleftouris George , Papachristos Ioannis V. , Papakostidis Costas , Giannoudis Peter V.
Published: November 2019

Background: Research has suggested that bone fractures can hinder the health status of patients’ life. However, limited research has examined the impact that the healing process of a fracture has on the physical health and psychological state of individuals, particularly in considering the short- and long-term impact of having a fracture that fails to heal and drops into a non-union. The aim of this systematic review is to better understand the impact of fracture non-union to physical health and to respective psychological outcomes. Methods: Electronic databases ‘PubMed’, ‘Cochrane’, ‘PsycInfo’, ‘Medline’, ‘Embase’, ‘Web of Science’, and ‘CINAHL’ were used. Search terms used were nonunion OR non-union OR “non union” OR “long bone” OR “delayed union” AND “quality of life” OR qol OR depression OR anxiety OR psycholog* OR PTSD OR “post-traumatic stress disorder”. Studies published in the years 1995 to 2018 were included. Two independent reviewers carried out screening and data extraction. Studies were included if (1) participants were adult (human) patients with a traumatic non-union secondary to fracture/s; (2) outcomes measured included physical health and psychological wellbeing (e.g., PTSD, psychological trauma, depression, anxiety, etc.). Studies received emphasis if they compared those outcomes between: (1) The “non-union” group to a normative, matched population and (2) the “non-union group” to the same group after union was achieved. However, studies that did not use comparison groups were also included. Results: Out of the 1896 papers identified from our thorough literature search, 13 met the inclusion criteria. Quality assessment was done by the Methodological Index for Non-Randomized Studies (MINORS). Findings suggested that non-unions had a detrimental impact on physical health, and psychological difficulties often after recovery. Conclusions: Patients who experience a long bone non-union are at risk of greater psychological distress and lower physical health status. There is a need for early identification of psychological distress in patients with fracture non-unions and psychological provision should become part of the available treatment.
Effects of Volatile versus Total Intravenous Anesthesia on Occurrence of Myocardial Injury after Non-Cardiac Surgery
Authors: Kwon Ji-Hye , Park Jungchan , Lee Seung-Hwa , Oh Ah-ran , Lee Jong-Hwan , Min Jeong Jin
Published: November 2019

The cardioprotective effects of volatile anesthetics versus total intravenous anesthesia (TIVA) are controversial, especially in patients undergoing non-cardiac surgery. Using current generation high-sensitivity cardiac troponin (hs-cTn), we aimed to evaluate the effect of anesthetics on the occurrence of myocardial injury after non-cardiac surgery (MINS). From February 2010 to December 2016, 3555 patients without preoperative hs-cTn elevation underwent non-cardiac surgery under general anesthesia. Patients were grouped according to anesthetic agent; 659 patients were classified into a propofol-remifentanil total intravenous anesthesia (TIVA) group, and 2896 patients were classified into a volatile group. To balance the use of remifentanil between groups, a balanced group (n = 1622) was generated with patients who received remifentanil infusion in the volatile group, and two separate comparisons were performed (TIVA vs. volatile and TIVA vs. balanced). The primary outcome was occurrence of MINS, defined as rise of hs-cTn I ≥ 0.04 ng/mL within postoperative 48 hours. The secondary outcomes were 30-day mortality, postoperative acute kidney injury (AKI), and adverse events during hospital stay (mortality, type I myocardial infarction (MI), and new-onset arrhythmia). In propensity-matched analyses, the occurrence of MINS was lower in the TIVA group compared to the volatile group (OR 0.642; 95% CI 0.450–0.914; p = 0.014). However, after balancing the use of remifentanil, there was no difference between groups in the risk of MINS (OR 0.832; 95% CI 0.554–1.251; p-value = 0.377). There were no significant associations between the two groups in type 1 MI, new-onset atrial fibrillation, in-hospital and 30-day mortality before and after balancing the use of remifentanil. However, the incidence of postoperative AKI was lower in the TIVA group (OR 0.362; 95% CI 0.194–0.675; p-value = 0.001). After balancing the use of remifentanil, volatile anesthesia and TIVA showed comparable effects on MINS in patients undergoing non-cardiac surgery without preoperative myocardial injury. Further studies are needed on the benefit of remifentanil infusion.
A Perspective on Microneedle-Based Drug Delivery and Diagnostics in Paediatrics
Authors: Pires Liliana R , Vinayakumar KB , Turos Maria , Miguel Verónica , Gaspar João
Published: November 2019

Microneedles (MNs) have been extensively explored in the literature as a means to deliver drugs in the skin, surpassing the stratum corneum permeability barrier. MNs are potentially easy to produce and may allow the self-administration of drugs without causing pain or bleeding. More recently, MNs have been investigated to collect/assess the interstitial fluid in order to monitor or detect specific biomarkers. The integration of these two concepts in closed-loop devices holds the promise of automated and minimally invasive disease detection/monitoring and therapy. These assure low invasiveness and, importantly, open a window of opportunity for the application of population-specific and personalised therapies.
The Influence of WEDM Parameters Setup on the Occurrence of Defects When Machining Hardox 400 Steel
Authors: Mouralova Katerina , Prokes Tomas , Benes Libor , Bednar Josef
Published: November 2019

The unconventional technology wire electrical discharge machining is a highly used technology for producing precise and indented shaped parts of all materials that are at least electrically conductive. Its wide use makes this technology applicable in almost all branches of industry, even in the automotive industry, where the abrasion resistant material under investigation Hardox 400 steel is widely used for the manufacturing of truck bodies. The aim of this study was a comprehensive analysis of the machinability of this material using WEDM employing a 33−round experiment. Based on the change in machine parameters (pulse off time, gap voltage, discharge current, pulse on time, and wire feed), the cutting speed, the topography of machined surfaces, and the chemical composition of the workpiece surface, the morphology and condition of the subsurface layer including lamella production and a subsequent determination of the distribution of individual elements in the given area were analyzed. It has been found that during the machining of this steel, many defects occur in the subsurface layer of the material in the form of cracks with a depth of up to 22 µm and burned cavities. However, by appropriately adjusting the machine parameters, it was possible to completely remove these cracks.
Fluoride Release from Two High-Viscosity Glass Ionomers after Exposure to Fluoride Slurry and Varnish
Authors: Nassar Hani M. , Platt Jeffrey A.
Published: November 2019

The effect of brushing with different fluoride slurries on the fluoride release (FR) of different high-viscosity glass ionomer cements (GICs) was investigated. Fifty-eight discs were fabricated from two high-viscosity GICs (GC Fuji IX (F9) and 3M ESPE Ketac-fil (KF)). Five specimens from each brand were used to measure Vickers microhardness and the remaining were randomly assigned to one of four groups (n = 6) based on two-factor combinations: (1) fluoride concentration in the abrasive slurry (275 or 1250 ppm fluoride as NaF) and (2) immersion in a 22,500 ppm fluoride-containing solution. Specimens were brushed for a total of 20,000 strokes over 4 days with daily FR measurement. Data were analyzed using analysis of variance and Bonferroni tests (α = 0.05). Baseline FR and microhardness values were different between the two tested material brands. Exposure to a 22,500 ppm solution was associated with higher FR but not the exposure to 1250 ppm slurries. Brushing and immersion of glass ionomer cements in a 22,500 ppm F solution led to higher FR that was more sustained for KF. Type of the glass ionomer, progressive brushing, and fluoride varnish affected FR but not the fluoride content in the abrasive slurry.
Optical Studies of Nanodiamond-Tissue Interaction: Skin Penetration and Localization
Authors: Perevedentseva Elena , Ali Nsrein , Karmenyan Artashes , Skovorodkin Ilya , Prunskaite-Hyyryläinen Renata , Vainio Seppo , Cheng Chia-Liang , Kinnunen Matti
Published: November 2019

In this work, several optical-spectroscopic methods have been used to visualize and investigate the penetration of diamond nanoparticles (NPs) of various sizes (3–150 nm), surface structures and fluorescence properties into the animal skin in vitro. Murine skin samples have been treated with nanodiamond (ND) water suspensions and studied using optical coherence tomography (OCT), confocal and two-photon fluorescence microscopy and fluorescence lifetime imaging (FLIM). An analysis of the optical properties of the used nanodiamonds (NDs) enables the selection of optimal optical methods or their combination for the study of nanodiamond–skin interaction. Among studied NDs, particles of 100 nm in nominal size were shown to be appropriate for multimodal imaging using all three methods. All the applied NDs were able to cross the skin barrier and penetrate the different layers of the epidermis to finally arrive in the hair follicle niches. The results suggest that NDs have the potential for multifunctional applications utilizing multimodal imaging.
Nonlinear Impulsive Multi-Order Caputo-Type Generalized Fractional Differential Equations with Infinite Delay
Authors: Ahmad Bashir , Alghanmi Madeaha , Alsaedi Ahmed , Agarwal Ravi P.
Published: November 2019

We establish sufficient conditions for the existence of solutions for a nonlinear impulsive multi-order Caputo-type generalized fractional differential equation with infinite delay and nonlocal generalized integro-initial value conditions. The existence result is proved by means of Krasnoselskii’s fixed point theorem, while the contraction mapping principle is employed to obtain the uniqueness of solutions for the problem at hand. The paper concludes with illustrative examples.
Removing Twins in Graphs to Break Symmetries
Authors: González Antonio , Puertas María Luz
Published: November 2019

Determining vertex subsets are known tools to provide information about automorphism groups of graphs and, consequently about symmetries of graphs. In this paper, we provide both lower and upper bounds of the minimum size of such vertex subsets, called the determining number of the graph. These bounds, which are performed for arbitrary graphs, allow us to compute the determining number in two different graph families such are cographs and unit interval graphs.
Reliability Evaluation for a Stochastic Flow Network
Based on Upper and Lower Boundary Vectors
Authors: Huang Ding-Hsiang , Huang Cheng-Fu , Lin Yi-Kuei
Published: November 2019

For stochastic flow network (SFN), given all the lower (or upper) boundary points, the classic problem is to calculate the probability that the capacity vectors are greater than or equal to the lower boundary points (less than or equal to the upper boundary points). However, in some practical cases, SFN reliability would be evaluated between the lower and upper boundary points at the same time. The evaluation of SFN reliability with upper and lower boundary points at the same time is the focus of this paper. Because of intricate relationships among upper and lower boundary points, a decomposition approach is developed to obtain several simplified subsets. SFN reliability is calculated according to these subsets by means of the inclusion-exclusion principle. Two heuristic options are then established in order to calculate SFN reliability in an efficient direction based on the lower and upper boundary points.
Calcium-Bearing Minerals Transformation during Underground Coal Gasification
Authors: Liu Shuqin , Ma Weiping
Published: November 2019

Calcium-bearing minerals are one of the main typical minerals in coal and coal ash. In the process of coal thermal conversion, calcium-bearing minerals undergo different morphological transformation in which the reaction temperature, pressure, and atmosphere are important factors affecting their transformation. The reaction process of underground coal gasification (UCG) could be clearly divided into pyrolysis, reduction, and oxidation and the typical calcium-bearing minerals are expected to indicate the actual reaction conditions of UCG. A high-calcium coal, Zhundong coal, was used in this research. The products of UCG were prepared and the minerals were identified by X-ray diffraction (XRD) and a scanning electron microscope coupled with an energy-dispersive spectrometer (SEM-EDS). The thermodynamic calculation was used to assist in understanding the transformation behaviors of calcium-bearing minerals. The experimental results show that the calcium-bearing mineral is gradually converted from gypsum (CaSO4·2H2O) in the raw coal into anhydrite (CaSO4) during the pyrolysis process. In the reduction stage, anhydrite reacts with the reducing gas (CO) to produce oldhamite (CaS), and the oldhamite is stably present in the reduction ash. During the oxidation process, oldhamite is first transformed into CaSO4, and then CaSO4 is converted into CaO. Finally, CaO reacts with Al2O3 and SiO2 to produce gehlenite (Ca2Al2SiO7) at 1100 °C. As the oxidation temperature rises to 1400 °C, gehlenite is transformed into the thermodynamically stable anorthite (CaAl2Si2O8). With the further progress of the reaction, anorthite will co-melt with iron-bearing minerals above 1500 °C. The ternary phase diagram of SiO2–Al2O3–CaO system proves that anorthite and gehlenite are the typical high-temperature calcium-bearing minerals when the mole fraction of SiO2 is higher than 0.6. Moreover, the gehlenite is converted to anorthite with the temperature rise, which is consistent with experimental results. This study provides a scientific basis for understanding the UCG reaction conditions.
A Method to Reuse Archived H&E Stained Histology Slides for a Multiplex Protein Biomarker Analysis
Authors: Hinton James P. , Dvorak Katerina , Roberts Esteban , French Wendy J. , Grubbs Jon C. , Cress Anne E. , Tiwari Hina A. , Nagle Raymond B.
Published: November 2019

Archived Hematoxylin and Eosin (H&E) stained pathology slides are routinely stored to index formalin-fixed paraffin-embedded (FFPE) sample tissue blocks. FFPE blocks are clinically annotated human tumor specimens that can be valuable in studies decades after the tissue is collected. If stored properly, they have the potential to yield a valuable number of serial sectioned slides for diagnostic or research purposes. However, some retrospective studies are limited in scope because the tissue samples have been depleted or not enough material is available in stored blocks for serial sections. The goal of these studies was to determine if archived H&E-stained slides can be directly reutilized by optimizing methods to de-stain and then re-stain the H&E stained slides to allow the detection of several biomarkers of interest using a conjugated antibody with chromogen multiplex immunohistochemistry procedure. This simple but innovative procedure, combined with image analysis techniques, demonstrates the ability to perform precise detection of relevant markers correlated to disease progression in initially identified tumor regions in tissue. This may add clinical value in retaining H&E slides for further use.
Synergistical Use of Electrostatic and Hydrophobic Interactions for the Synthesis of a New Class of Multifunctional Nanohybrids: Plasmonic Magneto-Liposomes
Authors: Știufiuc Gabriela Fabiola , Nițică Ștefan , Toma Valentin , Iacoviță Cristian , Zahn Dietrich , Tetean Romulus , Burzo Emil , Lucaciu Constantin Mihai , Știufiuc Rareș Ionuț
Published: November 2019

By carefully controlling the electrostatic interactions between cationic liposomes, which already incorporate magnetic nanoparticles in the bilayers, and anionic gold nanoparticles, a new class of versatile multifunctional nanohybrids (plasmonic magneto-liposomes) that could have a major impact in drug delivery and controlled release applications has been synthesized. The experimental results confirmed the successful synthesis of hydrophobic superparamagnetic iron oxide nanoparticles (SPIONs) and polyethylene glycol functionalized (PEGylated) gold nanoparticles (AuNPs). The SPIONs were incorporated in the liposomal lipidic bilayers, thus promoting the formation of cationic magnetoliposomes. Different concentrations of SPIONs were loaded in the membrane. The cationic magnetoliposomes were decorated with anionic PEGylated gold nanoparticles using electrostatic interactions. The successful incorporation of SPIONs together with the modifications they generate in the bilayer were analyzed using Raman spectroscopy. The plasmonic properties of the multifunctional nanohybrids were investigated using UV-Vis absorption and (surface-enhanced) Raman spectroscopy. Their hyperthermic properties were recorded at different frequencies and magnetic field intensities. After the synthesis, the nanosystems were extensively characterized in order to properly evaluate their potential use in drug delivery applications and controlled release as a result of the interaction with an external stimulus, such as an NIR laser or alternating magnetic field.
A Facile, One-Step Synthesis of Silicon/Silicon Carbide/Carbon Nanotube Nanocomposite as a Cycling-Stable Anode for Lithium Ion Batteries
Authors: Zhang Yi , Hu Kai , Zhou Yunlei , Xia Yingbin , Yu Nengfei , Wu Guanglei , Zhu Yusong , Wu Yuping , Huang Haibo
Published: November 2019

Silicon/carbon nanotube (Si/CNTs) nanocomposite is a promising anode material for lithium ion batteries (LIBs). Challenges related to the tricky synthesis process, as well as the weak interaction between Si and CNTs, hinder practical applications. To address these issues, a facile, one-step method to synthesize Si/CNTs nanocomposite by using silica (SiO2) as a reactant via a magnesium reduction process was developed. In this synthesis, the heat released enables the as-obtained Si to react with CNTs in the interfacial region to form silicon carbide (SiC). By virtue of the unique structure composed of Si nanoparticles strongly anchored to conductive CNTs network with stable Si–C chemical bonding, the Si/SiC/CNT nanocomposite delivers a stable capacity of ~1100 mAh g−1 and a capacity retention of about 83.8% after 200 cycles at a current density of 100 mA g−1. Our studies may provide a convenient strategy for the preparation of the Si/C anode of LIBs.
Body Fluid-Independent Effects of Dietary Salt Consumption in Chronic Kidney Disease
Authors: Oppelaar Jetta J. , Vogt Liffert
Published: November 2019

The average dietary salt (i.e., sodium chloride) intake in Western society is about 10 g per day. This greatly exceeds the lifestyle recommendations by the WHO to limit dietary salt intake to 5 g. There is robust evidence that excess salt intake is associated with deleterious effects including hypertension, kidney damage and adverse cardiovascular health. In patients with chronic kidney disease, moderate reduction of dietary salt intake has important renoprotective effects and positively influences the efficacy of common pharmacological treatment regimens. During the past several years, it has become clear that besides influencing body fluid volume high salt also induces tissue remodelling and activates immune cell homeostasis. The exact pathophysiological pathway in which these salt-induced fluid-independent effects contribute to CKD is not fully elucidated, nonetheless it is clear that inflammation and the development of fibrosis play a major role in the pathogenic mechanisms of renal diseases. This review focuses on body fluid-independent effects of salt contributing to CKD pathogenesis and cardiovascular health. Additionally, the question whether better understanding of these pathophysiological pathways, related to high salt consumption, might identify new potential treatment options will be discussed.
PipeNig®-FL, a Fluid Extract of Black Pepper (Piper Nigrum L.) with a High Standardized Content of Trans-β-Caryophyllene, Reduces Lipid Accumulation in 3T3-L1 Preadipocytes and Improves Glucose Uptake in C2C12 Myotubes
Authors: Geddo Federica , Scandiffio Rosaria , Antoniotti Susanna , Cottone Erika , Querio Giulia , Maffei Massimo E. , Bovolin Patrizia , Gallo Maria Pia
Published: November 2019

Trans-β-caryophyllene (BCP) is a natural sesquiterpene hydrocarbon with several important pharmacological activities, including antioxidant, anti-inflammatory, anticancer, and cardioprotective functions. These properties are mainly due to its selective interaction with the peripherally expressed cannabinoid receptor 2. In addition, BCP activates peroxisome proliferated activator receptors α and γ and inhibits the Toll-like receptor signaling pathway. Given the growing scientific interest in BCP, the aim of our study was to investigate the metabolic effects of a black pepper extract (PipeNig®-FL), containing a high standardized content of BCP. In particular our interest was focused on its potential activity on lipid accumulation and glucose uptake. The extract PipeNig®-FL was chemically characterized by gas chromatography–mass spectrometry (GC–MS) and gas chromatography with flame-ionization detection (GC–FID), confirming a high content (814 mg/g) of BCP. Experiments were performed on 3T3-L1 preadipocytes and on C2C12 myotubes. Lipid content following 3T3-L1 adipogenic differentiation was quantified with AdipoRed fluorescence staining. Glucose uptake and GLUT4 membrane translocation were studied in C2C12 myotubes with the fluorescent glucose analog 2-NBDG and by immunofluorescence analysis. Here we show that PipeNig®-FL reduces 3T3-L1 adipocyte differentiation and lipid accumulation. Moreover, acute exposure of C2C12 myotubes to PipeNig®-FL improves glucose uptake activity and GLUT4 migration. Taken together, these results reveal interesting and novel properties of BCP, suggesting potential applications in the prevention of lipid accumulation and in the improvement of glucose uptake.
Punicalagin Prevents Inflammation in LPS- Induced RAW264.7 Macrophages by Inhibiting FoxO3a/Autophagy Signaling Pathway
Authors: Cao Yuan , Chen Jihua , Ren Guofeng , Zhang Yahui , Tan Xiuying , Yang Lina
Published: November 2019

Punicalagin, a hydrolysable tannin of pomegranate juice, exhibits multiple biological effects, including inhibiting production of pro-inflammatory cytokines in macrophages. Autophagy, an intracellular self-digestion process, has been recently shown to regulate inflammatory responses. In this study, we investigated the anti-inflammatory potential of punicalagin in lipopolysaccharide (LPS) induced RAW264.7 macrophages and uncovered the underlying mechanisms. Punicalagin significantly attenuated, in a concentration-dependent manner, LPS-induced release of NO and decreased pro-inflammatory cytokines TNF-α and IL-6 release at the highest concentration. We found that punicalagin inhibited NF-κB and MAPK activation in LPS-induced RAW264.7 macrophages. Western blot analysis revealed that punicalagin pre-treatment enhanced LC3II, p62 expression, and decreased Beclin1 expression in LPS-induced macrophages. MDC assays were used to determine the autophagic process and the results worked in concert with Western blot analysis. In addition, our observations indicated that LPS-induced releases of NO, TNF-α, and IL-6 were attenuated by treatment with autophagy inhibitor chloroquine, suggesting that autophagy inhibition participated in anti-inflammatory effect. We also found that punicalagin downregulated FoxO3a expression, resulting in autophagy inhibition. Overall these results suggested that punicalagin played an important role in the attenuation of LPS-induced inflammatory responses in RAW264.7 macrophages and that the mechanisms involved downregulation of the FoxO3a/autophagy signaling pathway.
Qualitative and Quantitative Differences in Osmolytes Accumulation and Antioxidant Activities in Response to Water Deficit in Four Mediterranean Limonium Species
Authors: González-Orenga Sara , Al Hassan Mohamad , Llinares Josep V. , Lisón Purificación , López-Gresa M. Pilar , Verdeguer Mercedes , Vicente Oscar , Boscaiu Monica
Published: November 2019

Limonium is a genus represented in the Iberian Peninsula by numerous halophytic species that are affected in nature by salinity, and often by prolonged drought episodes. Responses to water deficit have been studied in four Mediterranean Limonium species, previously investigated regarding salt tolerance mechanisms. The levels of biochemical markers, associated with specific responses—photosynthetic pigments, mono- and divalent ions, osmolytes, antioxidant compounds and enzymes—were determined in the control and water-stressed plants, and correlated with their relative degree of stress-induced growth inhibition. All the tested Limonium taxa are relatively resistant to drought on the basis of both the constitutive presence of high leaf ion levels that contribute to osmotic adjustment, and the stress-induced accumulation of osmolytes and increased activity of antioxidant enzymes, albeit with different qualitative and quantitative induction patterns. Limonium santapolense activated the strongest responses and clearly differed from Limonium virgatum, Limonium girardianum, and Limonium narbonense, as indicated by cluster and principal component analysis (PCA) analyses in agreement with its drier natural habitat, and compared to that of the other plants. Somewhat surprisingly, however, L. santapolense was the species most affected by water deficit in growth inhibition terms, which suggests the existence of additional mechanisms of defense operating in the field that cannot be mimicked in greenhouses.