bluuebunny/crossref_metadata_2025 huggingface dataset:
Building Integrated Photovoltaics 4.0: Digitization of the Photovoltaic Integration in Buildings for a Resilient Infra at Large Scale
Authors: Singh Digvijay , Akram Shaik Vaseem , Singh Rajesh , Gehlot Anita , Buddhi Dharam , Priyadarshi Neeraj , Sharma Gulshan , Bokoro Pitshou N.
Published: August 2022

Building integrated photovoltaic (BIPV) systems have gained a lot of attention in recent years as they support the United Nations’ sustainable development goals of renewable energy generation and construction of resilient infrastructure. To make the BIPV system infra resilient, there is a need to adopt digital technologies such as the internet of things (IoT), artificial intelligence (AI), edge computing, unmanned aerial vehicles (UAV), and robotics. In this study, the current challenges in the BIPV system, such as the rise in the temperature of the PV modules, the occurrence of various faults, and the accumulation of dust particles over the module surface, have been identified and discussed based on the previous literature. To overcome the challenges, the significance and application of the integration of these digital technologies in the BIPV system are discussed along with the proposed architecture. Finally, the study discusses the vital recommendations for future directions, such as ML and DL for image enhancement and flaws detection in real-time image data; edge computing to implement DL for intelligent BIPV data analytics; fog computing for 6G assisted IoT network in BIPV; edge computing integration in UAV for intelligent automation and detection; augmented reality, virtual reality, and digital twins for virtual BIPV systems with research challenges of real-time implementation in the BIPV.
Neurogenetic and Epigenetic Aspects of Cannabinoids
Authors: Dennen Catherine A. , Blum Kenneth , Bowirrat Abdalla , Khalsa Jag , Thanos Panayotis K. , Baron David , Badgaiyan Rajendra D. , Gupta Ashim , Braverman Eric R. , Gold Mark S.
Published: August 2022

Cannabis is one of the most commonly used and abused illicit drugs in the world today. The United States (US) currently has the highest annual prevalence rate of cannabis consumption in the world, 17.9% in individuals aged 12 or older, and it is on the rise. With increasing cannabis use comes the potential for an increase in abuse, and according to the Substance Abuse and Mental Health Services Administration (SAMHSA), approximately 5.1% of Americans had Cannabis Use Disorder (CUD) in 2020. Research has shown that genetics and epigenetics play a significant role in cannabis use and CUD. In fact, approximately 50–70% of liability to CUD and 40–48% of cannabis use initiation have been found to be the result of genetic factors. Cannabis usage and CUD have also been linked to an increased risk of psychiatric disorders and Reward Deficiency Syndrome (RDS) subsets like schizophrenia, depression, anxiety, and substance use disorder. Comprehension of the genetic and epigenetic aspects of cannabinoids is necessary for future research, treatment plans, and the production of pure cannabinoid compounds, which will be essential for FDA approval. In conclusion, having a better understanding of the epigenetic and genetic underpinnings of cannabis use, CUD, and the endocannabinoid system as a whole will aid in the development of effective FDA-approved treatment therapies and the advancement of personalized medicine.
Soil Microbial Community Responds to Elevation Gradient in an Arid Montane Ecosystem in Northwest China
Authors: Yang Junlong , Yu Jian , Wang Jifei , Li Xiaowei
Published: August 2022

This study sought to clarify the composition of soil fungal and bacterial communities along an elevation gradient in an arid montane ecosystem as well as the influence of environmental factors (soil properties, climate, topography, and plant diversity) upon soil microbial community structures. Four vegetation types—montane desert steppe (mean elevation: 1761 m), montane shrub (mean elevation: 2077 m), subalpine coniferous forest (mean elevation: 2485 m), and subalpine shrub (mean elevation: 2903 m)—were sampled on the western slope of the Helan Mountains. The 16SrRNA gene and ITS1 were performed by single-molecule real-time (SMRT) sequencing with the PacBio sequencing platform. The Chao1 and Shannon–Wiener diversity of soil fungi and bacteria were more diverse in the soil of the lower elevation gradient compared to that of the upper one. Differences in abundance among phyla were found via One-way ANOVA(analysis of variance), yet the dominant soil fungal phyla (Ascomycota, Basidiomycota, and Mortierellomycota) and bacterial phyla (Proteobacteria, Acidobacteria, and Bacteroidetes) were the same across the elevation gradient. Pearson correlations and redundancy analysis (RDA) indicated that plant diversity (Shannon–Wiener diversity [H] and Margalef richness [D]), solar radiation, mean annual temperature, soil organic matter, soil moisture content, slope, mean annual precipitation, and elevation all significantly influenced the community composition of different soil fungal and bacterial phyla. Although plant diversity significantly affects fungal and bacterial diversity, the results imply that the influence of plant functional diversity on soil microbial community variation should not be ignored.
Effects of Fermented Seaweed Fertilizer Treatment on Paddy Amino Acid Content and Rhizosphere Microbiome Community
Authors: Prasedya Eka Sunarwidhi , Kurniawan Nanda Sofian Hadi , Ardiana Nur , Ilhami Bq Tri Khairina , Mulyaningsih Tri , Astuti Sri Puji , Jupri Ahmad , Nikmatullah Aluh , Jaya I Komang Damar , Widyastuti Sri
Published: August 2022

Seaweed has often been reported on for it potential bioresources for fertilizers to improve crop productivity and reduce the use of chemical fertilizers (CF). However, little is known about the nutritional status of the crop grown with the implementation of seaweed fertilizers (SF). In this study, the amino acid content of rice produced by SF implementation was evaluated. Furthermore, the rhizosphere bacterial community was also investigated. The paddy seedlings were divided into five groups, control (C0), chemical fertilizer (CF), seaweed fertilizer (SF), chemical and seaweed fertilizer combination 25:75 (CFSF1), and chemical and fertilizer combination 50:50 (CFSF2). The CFSF2 group shown significantly better growth characteristics compared to other groups. Based on the concentration of macronutrients (N, P, K) in paddy leaf, CFSF2 also shown the best results. This also correlates with the abundant amino acid composition in CFSF2 in almost all tested amino acids, namely, serine, phenylalanine, isoleucine, valine, glycine, tyrosine, proline, threonine, histidine, and arginine. Interestingly, beneficial bacteria Rhizobiales were significantly higher in CFSF2-treated soil (58%) compared to CF (29%). Another important group, Vicinamibacterales, was also significantly higher in CFSF2 (58%) compared to CF (7%). Hence, these potentially contributed to the high rice amino acid content and yield in the CFSF2-treated paddy. However, further field-scale studies are needed to confirm the bioindustrial application of seaweed in agricultural systems.
Structural Characteristics and the Antioxidant and Hypoglycemic Activities of a Polysaccharide from Lonicera caerulea L. Pomace
Authors: Pei Fangyi , Lv Yuze , Cao Xinbo , Wang Xuemeng , Ren Yanxin , Ge Jingping
Published: August 2022

In this study, a novel polysaccharide, LPP, was obtained from Lonicera caerulea L. pomace by ultrasonic-assisted heating and was purified by Sephadex G-100. The structural characteristics of LPP showed that the molecular weight (Mw) was 8.53 × 104 Da; that it was mainly composed of galacturonic acid, followed by galactose; that it possessed the characteristic functional groups of polysaccharides; and that it had an absence of O-glycosidic bonds and crystalline and triple helix structures. Furthermore, LPP exhibited a favorable thermodynamic stability and antioxidant, hypoglycemic, and hypolipidemic activities in a dose-dependent manner in vitro, demonstrating that LPP can be used as an agent to regulate glycolipid metabolism. Additionally, the relationship between its bio-activities is discussed in this paper. The results revealed that the RP, •OH, and NO2− radicals had synergistic promoting effects, and polysaccharides with a strong antioxidant ability may have excellent hypoglycemic and hypolipidemic effects. Collectively, these results suggest that LPP has a strong bio-activity, and that Lonicera caerulea L. pomace can be used as a potential polysaccharide source.
Design of 5′-UTR to Enhance Keratinase Activity in Bacillus subtilis
Authors: Fang Jun , Zhou Guanyu , Ji Xiaomei , Zhang Guoqiang , Peng Zheng , Zhang Juan
Published: August 2022

Keratinase is an important industrial enzyme, but its application performance is limited by its low activity. A rational design of 5′-UTRs that increases translation efficiency is an important approach to enhance protein expression. Herein, we optimized the 5′-UTR of the recombinant keratinase KerZ1 expression element to enhance its secretory activity in Bacillus subtilis WB600 through Spacer design, RBS screening, and sequence simplification. First, the A/U content in Spacer was increased by the site-directed saturation mutation of G/C bases, and the activity of keratinase secreted by mutant strain B. subtilis WB600-SP was 7.94 times higher than that of KerZ1. Subsequently, the keratinase activity secreted by the mutant strain B. subtilis WB600-SP-R was further increased to 13.45 times that of KerZ1 based on the prediction of RBS translation efficiency and the multi-site saturation mutation screening. Finally, the keratinase activity secreted by the mutant strain B. subtilis WB600-SP-R-D reached 204.44 KU mL−1 by reducing the length of the 5′ end of the 5′-UTR, which was 19.70 times that of KerZ1. In a 5 L fermenter, the keratinase activity secreted by B. subtilis WB600-SP-R-D after 25 h fermentation was 797.05 KU mL−1, which indicated its high production intensity. Overall, the strategy of this study and the obtained keratinase mutants will provide a good reference for the expression regulation of keratinase and other industrial enzymes.
Antimicrobial Activity of Some Plant Extracts and Their Applications in Homemade Tomato Paste and Pasteurized Cow Milk as Natural Preservatives
Authors: Atwaa El Sayed Hassan , Shahein Magdy Ramadan , Radwan Hanan A. , Mohammed Nahed S. , Aloraini Maha A. , Albezrah Nisreen Khalid Aref , Alharbi Maha A. , Sayed Haitham Helmy , Daoud Mamdouh Abdelmegid , Elmahallawy Ehab Kotb
Published: August 2022

Synthetic chemical preservatives are widely used in the food industry to delay the deterioration caused by microbial growth, enzyme activities and oxidation reactions. The last few decades have witnessed marked interest in finding natural food preservatives due to the potential health damage of synthetic preservatives; consumers have become skeptical of consuming foods containing these additives. Polyphenols used as natural preservatives that can be extracted from fruits, vegetables, herbs and spices provide the best alternative for partial or complete replacement of their synthetic analogues. The present study’s emphasis was on employing different plant extracts to be efficiently used as antimicrobial agents for developing replacements for the synthetic chemical additives in food products. The study also investigated the antimicrobial potentialities of five medicinal plants, widely used in Egypt (sumac, tamarind, rosemary, roselle and lemon) against six microbial markers (E. coli, P. aeruginosae, B. subtilis, S. aureus, Penicillium sp. and A. niger.). Sumac extracts showed the best activity against all tested microorganisms, producing the widest inhibition zones ranging from 14 to 45 mm, followed by tamarind and roselle extracts, with inhibition zones ranging from 8–36 and 8–34 mm, respectively. On the other hand, extracts of rosemary and lemon showed variable antimicrobial activity. All extracts from all tested plants were less active against fungal species than bacterial species. In all cases, the organic extracts (80% methanol, 80% ethanol) showed the same or greater activity than the aqueous extracts. In addition, the methanolic extracts showed the strongest and broadest spectrum. The most sensitive strain to plant extracts was B. subtilis, while the most resistant strain was P. aeruginosae. The MIC and MBC or MFC values of methanolic extracts were assayed using the broth dilution method. Sumac extract showed the best activity against all tested microorganisms with the lowest values of MIC and MBC or MFC (from 0.260 to 0.877 and 0.310 to 1.316 mg/mL, respectively, for bacteria, and from 1.975 to 2.5 and 2.5 to 4.444 mg/mL, respectively, for fungi). Interestingly, the tested extracts inhibited microbial growth in tomato paste and pasteurized cow milk for a long storage period (increase shelf life) as compared to the control samples. In conclusion, herbal and spice extracts could be successfully applied as natural antimicrobials for the elimination of food borne microbes and pathogen growth.
Longleaf Pine Seedlings Are Extremely Resilient to the Combined Effects of Experimental Fire and Drought
Authors: Wilson Luke A. , Spencer Robert N. , Aubrey Doug P. , O’Brien Joseph J. , Smith Alistair M. S. , Thomas Ream W. , Johnson Daniel M.
Published: August 2022

The longleaf pine ecosystem is dependent on frequent fire. Climate change is expected to influence moisture availability and it is unclear how drought conditions may interact with prescribed fire to influence management objectives associated with maintaining longleaf pine ecosystems. This study aimed to understand the impacts of drought, fire intensity and their interaction on P. palustris grass-stage seedlings. We used droughted and well-watered P. palustris seedlings burned at two different fire intensity levels at an indoor combustion facility. Needle fuel moisture content of burned seedlings was not different between droughted and well-watered groups. Mortality and resprouting only occurred at fire intensity levels exceeding 3.5 MJ m−2 in combination with drought that resulted in predawn water potentials more negative than −1.7 MPa. Our observations of minimal mortality after exposing P. palustris seedlings to a range of fire intensities in a burn lab contrast the higher mortality observed in field studies for the species. Compared to seedlings and saplings of Western US Pinus species, this study demonstrates that P. palustris is considerably more resistant to the combined effects of high surface fire intensity and drought.
Fatty Acid Profile of Jumbo Squid (Dosidicus gigas) off the Peruvian Exclusive Economic Zone: Revealing the Variability of Feeding Strategies
Authors: Hu Guanyu , Zhao Zhenfang , Liu Bilin , Lin Dongming , Liang Jiawei , Fang Zhou , Chen Xinjun
Published: August 2022

Jumbo squid Dosidicus gigas play a critical role in the marine ecosystems and are an important commercial species in the East Pacific. Generally, three size groups have been distinguished on the basis of the mantle length (ML) of an adult. Here, fatty acid (FA) analyses of muscle tissues were used to explore the feeding strategies of D. gigas off the Peruvian exclusive economic zone in terms of different size groups and sexes. There was no significant difference in fatty acid composition between the small- and medium-sized groups, whereas the large-sized group differed significantly from other groups. The higher content of C20:4n6 and (C18:2n6 + C18:3n3) indicates that the large-sized group may feed more frequently in nearshore and deep waters. Furthermore, the niches of the three size groups were consistent with the results of fatty acid composition, with the large-sized group occupying the widest trophic niche, followed by the medium-sized group and then the small-sized group. In addition, there was evidence of trophic niche overlap between the small and medium groups. In terms of sexual variability of the small and medium groups, the fatty acid composition significantly differed between females and males. In terms of sexual variation of the large group, the fatty acid composition between females and males was similar, indicating that similar feeding strategies may be adopted by them. This study revealed the variability of the feeding strategies of three size groups of D. gigas off the Peruvian exclusive economic zone.
Authenticity in Olive Oils from an Empeltre Clonal Selection in Aragon (Spain): How Environmental, Agronomic, and Genetic Factors Affect Sterol Composition
Authors: Rey-Giménez Raquel , Sánchez-Gimeno Ana Cristina
Published: August 2022

Sterol composition is used as a “fingerprint” to demonstrate the authenticity of olive oils. Our study’s objective was to exhaustively characterize the sterol composition of Empeltre olive oils from clonal selection during the ripening period in 2017, 2018, and 2019. We likewise assessed the influence of crop year, fruit ripening, and clonal selection on the oils’ regulatory compliance in terms of sterol composition. Empeltre olive oils were shown to have medium-range β-sitosterol and Δ5-avenasterol content, along with elevated amounts of campesterol and Δ7-stigmastenol. A total of 26% and 12% of the samples were non-compliant in terms of apparent β-sitosterol and Δ7-stigmastenol, respectively. Crop year was the most influential factor in the case of most sterols. Clone type was the least influential factor, except in the case of campesterol. Olive maturity was only significant for Δ7-sterols. We likewise applied a discriminant analysis, with “crop year” as the grouping variable: 94.9% of the oils were thereby classified correctly.
Simultaneous Detection and Analysis of Free Amino Acids and Glutathione in Different Shrimp
Authors: Jin Yinzhe , Xu Minhua , Jin Yingshan , Deng Shanggui , Tao Ningping , Qiu Weiqiang
Published: August 2022

An amino acid analyzer method for the simultaneous determination of 20 free amino acids (FAAs) and glutathione (GSH) in Penaeus vannamei (PV), Penaeus vannamei, Penaeus hidulis (PH) and Penaeus japonicus (PJ) were developed. The effects of different concentrations of trichloroacetic acid (TCA) and ethanol on the extraction of free amino acids were investigated, and 120 g·L−1 TCA was found to be ideal. The target analytes were eluted in sodium citrate buffer B1 (pH = 3.3) containing 135 mL·L−1 ethanol and 1 mol·L−1 sodium hydroxide (7 mL) and at the optimizing conversion time of sodium citrate buffer B2 (pH = 3.2) and sodium citrate buffer B3 (pH = 4.0) of 5.6 min, and the effective separation was achieved within 29.5 min. The developed method showed good linearity (R2 ≥ 0.9991) in the range of 1–250 µg·mL−1 with good intra-day and inter-day precision (relative standard deviations ≤ 2.38%) and spike recovery (86.42–103.64%). GSH and cysteine were used to identify marine prawn and freshwater shrimp. Hydroxyproline and serine were used to distinguish PV and Macrobrachium nipponense (MN) from others, respectively. The highest content of the total FAAs was found in PV, and principal component analysis revealed that PV had the highest comprehensive score for FAAs and GSH. Arginine was found to have the greatest influence on shrimp flavor.
Efficacy and Safety of a Novel Therapeutic of Natural Origin (NTN) in Adult Patients with Lactose Intolerance: A Multicenter, Randomized, Crossover, Double-Blind, Placebo-Controlled Study
Authors: Pop Corina , Sporea Ioan , Santos Javier , Tudor Nicolae , Tiuca Nicoleta
Published: August 2022

Background: Film-forming substances, such as natural polysaccharides (NP) and pea proteins (PP), act as a protective barrier for treating various gastrointestinal conditions. We assessed the efficacy and safety of a novel therapeutic of natural origin (NTN) containing NP and PP for symptomatic treatment of lactose intolerance. Methods: In this multicenter, randomized, double-blind, parallel-group study, patients with lactose intolerance received NTN (n = 30) or placebo (n = 30) for 7 days, then the alternate treatment for 7 days. Patients rated their gastrointestinal symptoms using a 7-point Likert scale. The lactose hydrogen breath test was used to assess exhaled hydrogen. Results: NTN as primary or crossover treatment significantly improved patient-reported symptoms of bloating, distension, and abdominal pain. Abdominal pain also improved under primary treatment with placebo. Primary treatment with NTN, but not placebo, normalized mean exhaled hydrogen levels. In the group allocated initially to placebo, crossover to NTN attenuated the increase in hydrogen production. No treatment-related adverse effects were reported in either group. Conclusions: Subjective improvements in bloating, distension, and abdominal pain with NTN were supported by objective evidence of hydrogen production normalization. NTN appears to be a useful alternative to lactose avoidance or enzyme replacement in patients with lactose intolerance.
Association between Breakfast Meal Categories and Timing of Physical Activity of Japanese Workers
Authors: Roshanmehr Farnaz , Hayashi Katsuki , Tahara Yu , Suiko Takahiko , Nagamori Yuki , Iwai Takao , Shibata Shigenobu
Published: August 2022

Background: Breakfast is the most important meal of the day and has been associated with longevity. Regular breakfast consumers often have a healthy lifestyle, including a healthy diet and regular physical activity. Methods: We examined the association between breakfast type, chronotype (morningness-eveningness), and physical activity in 3395 Japanese workers using a cross-sectional web survey. Results: Participants who ate Japanese breakfasts showed an early chronotype, while those who ate breakfast cereal exhibited a later chronotype. Physical activity was positively associated with adopting a Japanese breakfast style. Japanese breakfast eaters performed physical activities from 6:00–9:00 compared with other breakfast eaters. Conclusion: Our findings suggest that eating a Japanese breakfast is associated with an earlier chronotype (morningness) and higher physical activity.
Symmetry Analysis and PT-Symmetric Extension of the Fifth-Order Korteweg-de Vries-Like Equation
Authors: Wang Gangwei , Shen Bo , He Mengyue , Guan Fei , Zhang Lihua
Published: August 2022

In the present paper, PT-symmetric extension of the fifth-order Korteweg-de Vries-like equation are investigated. Several special equations with PT symmetry are obtained by choosing different values, for which their symmetries are obtained simultaneously. In particular, for the particular equation, its conservation laws are obtained, including conservation of momentum and conservation of energy. Reciprocal Ba¨cklund transformations of conservation laws of momentum and energy are presented for the first time. The important thing is that for the special case of ϵ=3, the corresponding time fractional case are studied by Lie group method. And what is interesting is that the symmetry of the time fractional equation is obtained, and based on the symmetry, this equation is reduced to a fractional ordinary differential equation. Finally, for the general case, the symmetry of this equation is obtained, and based on the symmetry, the reduced equation is presented. Through the results obtained in this paper, it can be found that the Lie group method is a very effective method, which can be used to deal with many models in natural phenomena.
A Fractal Entropy-Based Effective Particle Model Used to Deduce Hydraulic Conductivity of Granular Soils
Authors: Zhang Gang , Wang Hongyu , Israr Jahanzaib , Ma Wenguo , Yang Youzhen , Ren Keliang
Published: August 2022

In this study, a rigorous mathematical approach used to compute an effective diameter based on particle size distribution (PSD) has been presented that can predict the hydraulic conductivity of granular soils with enhanced rigor. The PSD was discretized based on an abstract interval system of fractal entropy, while the effective diameter of soil was computed using the grading entropy theory. The comparisons between current entropy-based effective diameter (DE) and those computed using existing procedures show that the current DE can capture the particle size information of a given soil more accurately than others. Subsequently, the proposed DE was successfully implicated into Kozeny–Carman’s formula to deduce the saturated hydraulic conductivity of soils with enhanced accuracy. The proposed model was tested using current and previously published experimental data from literature. Not surprisingly, the results of the current model and those from previous experimental studies were found to be consistent, which can sufficiently verify the proposed entropy-based effective diameter model.
A Beginner’s Guide to the Characterization of Hydrogel Microarchitecture for Cellular Applications
Authors: Martinez-Garcia Francisco Drusso , Fischer Tony , Hayn Alexander , Mierke Claudia Tanja , Burgess Janette Kay , Harmsen Martin Conrad
Published: August 2022

The extracellular matrix (ECM) is a three-dimensional, acellular scaffold of living tissues. Incorporating the ECM into cell culture models is a goal of cell biology studies and requires biocompatible materials that can mimic the ECM. Among such materials are hydrogels: polymeric networks that derive most of their mass from water. With the tuning of their properties, these polymer networks can resemble living tissues. The microarchitectural properties of hydrogels, such as porosity, pore size, fiber length, and surface topology can determine cell plasticity. The adequate characterization of these parameters requires reliable and reproducible methods. However, most methods were historically standardized using other biological specimens, such as 2D cell cultures, biopsies, or even animal models. Therefore, their translation comes with technical limitations when applied to hydrogel-based cell culture systems. In our current work, we have reviewed the most common techniques employed in the characterization of hydrogel microarchitectures. Our review provides a concise description of the underlying principles of each method and summarizes the collective data obtained from cell-free and cell-loaded hydrogels. The advantages and limitations of each technique are discussed, and comparisons are made. The information presented in our current work will be of interest to researchers who employ hydrogels as platforms for cell culture, 3D bioprinting, and other fields within hydrogel-based research.
Formulation and Evaluation of Chitosan-Gelatin Thermosensitive Hydrogels Containing 5FU-Alginate Nanoparticles for Skin Delivery
Authors: Nawaz Asif , Ullah Shafi , Alnuwaiser Maha Abdallah , Rehman Fazal Ur , Selim Samy , Al Jaouni Soad K. , Farid Arshad
Published: August 2022

(1) Background: Chitosan-gelatin-based thermosensitive hydrogel containing 5FU-alginate nanoparticles was formulated for the effective and sustained delivery of 5FU to the skin. (2) Methods: Alginate, a polysaccharide was used for the formulation of nanoparticles using a spray drying technique. Size, zeta potential, and surface morphology were investigated using a zetasizer and scanning electron microscope. The hydrogel was fabricated using chitosan and gelatin. Several important analyses were used to characterize these prepared topical hydrogels. The pH, visual transparency, rheological behavior, and swelling index of the prepared hydrogels were evaluated. The in vitro release studies were performed at different pH (5.5 and 7.4) and temperature (32 and 37 °C) conditions using a Franz diffusion cell. Ex vivo permeation and in vivo studies were performed using Sprague Dawley rats. (3) Results: Results show that spherical nanoparticles were produced at sizes of 202–254 nm and with zeta potentials of −43 to −38 mV. The prepared nanoparticles were successfully incorporated into chitosan-gelatin-based hydrogels using a glycerol 2-phosphate disodium salt hydrates crosslinker. Drug polymers and excipients compatibility and formulation of hydrogels was confirmed by ATR-FTIR results. The pH of the prepared hydrogels was in accordance with the skin pH. The viscosity of prepared hydrogel increased with temperature increase and phase transition (sol-gel transition) occurred at 34 °C. The release of drug was sustained in case of nanoparticles incorporated hydrogels (5FU-Alg-Np-HG) as compared to nanoparticles (5FU-Alg-Np) and simple hydrogels (5FU-HG) (ANOVA; p < 0.05). The premature and initial burst release of 5FU was prevented using 5FU-Alg-Np-HG. The release mechanism of 5FU from the 5FU-Alg-Np-HG diffusion was followed by swelling and erosion, as suggested by Korsmeyer-Peppas model. The prepared hydrogel proved to be non-irritant. Ex vivo permeation study across rat’s skin suggests that permeability of nanoparticles (5FU-Alg-Np) was higher than the 5FU-Alg-Np-HG (ANOVA; p < 0.05). However, skin-related drug retention of 5FU-Alg-Np-HG was significantly higher than the 5FU solution, 5FU-Alg-Np, and 5FU-HG (ANOVA; p < 0.05). This was due to swelling of hydrogels in the lower layers of skin where the temperature is 37 °C. The higher concentration of 5FU in the skin is helpful for treatment of local skin cancer, such as melanoma, and actinic keratosis. In vivo results also confirmed maximum AUC, t1/2, and skin-related drug retention of 5FU-Alg-Np-HG. (4) Conclusions: Chitosan-gelatin-based hydrogels containing 5FU-Alg-Np possess exceptional properties, and can be used for the sustained delivery of 5FU for the treatment of local skin cancers.
Design and Development of Fluorinated and Biocide-Free Sol–Gel Based Hybrid Functional Coatings for Anti-Biofouling/Foul-Release Activity
Authors: Sfameni Silvia , Rando Giulia , Galletta Maurilio , Ielo Ileana , Brucale Marco , De Leo Filomena , Cardiano Paola , Cappello Simone , Visco Annamaria , Trovato Valentina , Urzì Clara , Plutino Maria Rosaria
Published: August 2022

Biofouling has destructive effects on shipping and leisure vessels, thus producing severe problems for marine and naval sectors due to corrosion with consequent elevated fuel consumption and higher maintenance costs. The development of anti-fouling or fouling release coatings creates deterrent surfaces that prevent the initial settlement of microorganisms. In this regard, new silica-based materials were prepared using two alkoxysilane cross-linkers containing epoxy and amine groups (i.e., 3-Glycidyloxypropyltrimethoxysilane and 3-aminopropyltriethoxysilane, respectively), in combination with two functional fluoro-silane (i.e., 3,3,3-trifluoropropyl-trimethoxysilane and glycidyl-2,2,3,3,4,4,5,5,6,6,7,7,8,8,9,9-hexadecafluorononylether) featuring well-known hydro repellent and anti-corrosion properties. As a matter of fact, the co-condensation of alkoxysilane featuring epoxide and amine ends, also mixed with two opportune long chain and short chain perfluorosilane precursors, allows getting stable amphiphilic, non-toxic, fouling release coatings. The sol–gel mixtures on coated glass slides were fully characterized by FT-IR spectroscopy, while the morphology was studied by scanning electron microscopy (SEM), and atomic force microscopy (AFM). The fouling release properties were evaluated through tests on treated glass slides in different microbial suspensions in seawater-based mediums and in seawater natural microcosms. The developed fluorinated coatings show suitable antimicrobial activities and low adhesive properties; no biocidal effects were observed for the microorganisms (bacteria).
Circular RNAs: New Players in Cardiomyopathy
Authors: Bagheri Moghaddam Maedeh , Maleki Majid , Oveisee Maziar , Bagheri Moghaddam Mahrokh , Arabian Maedeh , Malakootian Mahshid
Published: August 2022

Cardiomyopathies comprise a heterogeneous group of cardiac diseases identified by myocardium disorders and diminished cardiac function. They often lead to heart failure or heart transplantation and constitute one of the principal causes of morbidity and mortality worldwide. Circular RNAs (circRNAs) are a novel type of noncoding RNAs. They are covalently closed and single-stranded and derived from the exons and introns of genes by alternative splicing. This specific structure renders them resistant to exonuclease digestion. Many recent studies have demonstrated that circRNAs are highly abundant and conserved and can play central roles in biological functions such as microRNA (miRNA) sponging, splicing, and transcription regulation. Emerging evidence indicates that circRNAs can play significant roles in cardiovascular diseases, including cardiomyopathies. In this review, we briefly describe the current understanding regarding the classification, nomenclature, characteristics, and function of circRNAs and report recent significant findings concerning the roles of circRNAs in cardiomyopathies. Furthermore, we discuss the clinical application potential of circRNAs as the therapeutic targets and diagnostic biomarkers of cardiomyopathies.
Whole-Genome Sequencing Reveals the Genomic Characteristics and Selection Signatures of Hainan Black Goat
Authors: Chen Qiaoling , Chai Yuan , Zhang Wencan , Cheng Yiwen , Zhang Zhenxing , An Qi , Chen Si , Man Churiga , Du Li , Zhang Wenguang , Wang Fengyang
Published: August 2022

Goats have become one of the most adaptive and important livestock species distributed in developing countries in recent years. The Hainan Black goat is a native goat breed of the Hainan region that is generally well-liked by the local population and is thus raised in large numbers. However, the genomic diversity and selective signals of the Hainan Black goat have not been clearly elucidated yet. Therefore, in this study, we performed whole-genome resequencing of 16 Hainan Black goats and compared the results with those of 71 goats of 6 other breeds from different geographic regions. Principal component analysis (PCA) and phylogenetic analysis identified seven lineages for all goats. Hainan Black goats showed the most similarity with Leizhou goats and the least similarity with Boer goats. Selective sweep analysis identified candidate genes associated with various functions, including immune resistance to disease (TNFAIP2 (TNF alpha induced protein 2) and EXOC3L4 (exocyst complex component 3 like 4)), melanin biosynthetic process (CDH15 (cadherin 15), ASIP (agouti signaling protein), and PARD3 (par-3 family cell polarity regulator)), and light sensitivity (CNGB3 (cyclic nucleotide gated channel subunit beta 3) and CNBD1 (cyclic nucleotide binding domain containing 1)), underlying strong selection signatures in Hainan Black goats. The melanin biosynthetic process, circadian entrainment, regulation of cyclic adenosine 3,5-monophosphate (cAMP)-mediated signaling, and the Rap-1 signaling pathway were significantly enriched in Hainan Black and Alashan Cashmere goats. This result may be important for understanding each trait. Selection signature analysis revealed candidate single nucleotide polymorphisms (SNPs) and genes correlated with the traits of Hainan Black goats. Collectively, our results provide valuable insights into the genetic basis of specific traits correlated with the Hainan island climate, artificial selection in certain local goat breeds, and the importance of protecting breed resources.
Genome-Wide Identification and Expression Analysis of WRKY Transcription Factors in Akebiatrifoliata: A Bioinformatics Study
Authors: Zhu Jun , Zhong Shengfu , Guan Ju , Chen Wei , Yang Hao , Yang Huai , Chen Chen , Tan Feiquan , Ren Tianheng , Li Zhi , Li Qing , Luo Peigao
Published: August 2022

WRKY transcription factors have been found in most plants and play an important role in regulating organ growth and disease response. Outlining the profile of WRKY genes is a very useful project for studying morphogenesis and resistance formation. In the present study, a total of 63 WRKY genes consisting of 13 class I, 41 class II, and 9 class III genes were identified from the newly published A. trifoliata genome, of which 62 were physically distributed on all 16 chromosomes. Structurally, two AkWRKY genes (AkWRKY6 and AkWRKY52) contained four domains, and AkWRKY17 lacked the typical heptapeptide structure. Evolutionarily, 42, 16, and 5 AkWRKY genes experienced whole genome duplication (WGD) or fragmentation, dispersed duplication, and tandem duplication, respectively; 28 Ka/Ks values of 30 pairs of homologous genes were far lower than 1, while those of orthologous gene pairs between AkWRKY41 and AkWRKY52 reached up to 2.07. Transcriptome analysis showed that many of the genes were generally expressed at a low level in 12 fruit samples consisting of three tissues, including rind, flesh, and seeds, at four developmental stages, and interaction analysis between AkWRKY and AkNBS genes containing W-boxes suggested that AkWRKY24 could play a role in plant disease resistance by positively regulating AkNBS18. In summary, the WRKY gene family of A. trifoliata was systemically characterized for the first time, and the data and information obtained regarding AkWRKY could be very useful in further theoretically elucidating the molecular mechanisms of plant development and response to pathogens and practically improving favorable traits such as disease resistance.
Development and Characterization of Microsatellite Markers Based on the Chloroplast Genome of Tree Peony
Authors: Guo Qi , Guo Lili , Li Yuying , Yang Haijing , Hu Xiaoliang , Song Chengwei , Hou Xiaogai
Published: August 2022

Tree peony (Paeonia suffruticosa Andr.) is a famous ornamental and medicinal flowering species. However, few high-efficiency chloroplast microsatellite markers have been developed for it to be employed in taxonomic identifications and evaluation of germplasm resources to date. In the present study, a total of 139 cpSSR loci were identified across eleven tree peony plastomes. Dinucleotide repeat SSRs (97.12%) were most abundantly repeated for the AT motif (58.27%), followed by the TA motif (30.94%) and the TC motif (7.91%). Twenty-one primer pairs were developed, and amplification tests were conducted for nine tree peony individuals. Furthermore, 19 cpSSR markers were amplified on 60 tree peony accessions by a capillary electrophoresis test. Of 19 cpSSR markers, 12 showed polymorphism with different alleles ranging from 1.333 to 3.000. The Shannon’s information index and polymorphism information content values ranged from 0.038 to 0.887 (mean 0.432) and 0.032 to 0.589 (mean 0.268), respectively. The diversity levels for twelve loci ranged from 0.016 (at loci cpSSR-8 and cpSSR-26) to 0.543 (at locus cpSSR-15), averaging 0.268 for all loci. A total of 14 haplotypes (23.33%) were detected in the three populations. The haplotypic richness ranged from 0.949 to 1.751, with a mean of 1.233 per population. The genetic relationship suggested by the neighbor-joining-based dendrogram divided the genotypes into two clusters. The Jiangnan population was allotted to Cluster II, and the other two populations were distributed into both branches. These newly developed cpSSRs can be utilized for future breeding programs, population genetics investigations, unraveling the genetic relationships between related species, and germplasm management.
In-Silico Characterization of Estrogen Reactivating β-Glucuronidase Enzyme in GIT Associated Microbiota of Normal Human and Breast Cancer Patients
Authors: Muccee Fatima , Ghazanfar Shakira , Ajmal Wajya , Al-Zahrani Majid
Published: August 2022

Estrogen circulating in blood has been proved to be a strong biomarker for breast cancer. A β-glucuronidase enzyme (GUS) from human gastrointestinal tract (GIT) microbiota including probiotics has significant involvement in enhancing the estrogen concentration in blood through deconjugation of glucuronidated estrogens. The present project has been designed to explore GIT microbiome-encoded GUS enzymes (GUSOME) repertoire in normal human and breast cancer patients. For this purpose, a total of nineteen GUS enzymes from human GIT microbes, i.e., seven from healthy and twelve from breast cancer patients have been focused on. Protein sequences of enzymes retrieved from UniProt database were subjected to ProtParam, CELLO2GO, SOPMA (secondary structure prediction method), PDBsum (Protein Database summaries), PHYRE2 (Protein Homology/AnalogY Recognition Engine), SAVES v6.0 (Structure Validation Server), MEME version 5.4.1 (Multiple Em for Motif Elicitation), Caver Web server v 1.1, Interproscan and Predicted Antigenic Peptides tool. Analysis revealed the number of amino acids, isoelectric point, extinction coefficient, instability index and aliphatic index of GUS enzymes in the range of 586–795, 4.91–8.92, 89,980–155,075, 25.88–40.93 and 71.01–88.10, respectively. Sub-cellular localization of enzyme was restricted to cytoplasm and inner-membrane in case of breast cancer patients’ bacteria as compared to periplasmic space, outer membrane and extracellular space in normal GIT bacteria. The 2-D structure analysis showed α helix, extended strand, β turn and random coil in the range of 27.42–22.66%, 22.04–25.91%, 5.39–8.30% and 41.75–47.70%, respectively. The druggability score was found to be 0.05–0.45 and 0.06–0.80 in normal and breast cancer patients GIT, respectively. The radius, length and curvature of catalytic sites were observed to be 1.1–2.8 Å, 1.4–15.9 Å and 0.65–1.4, respectively. Ten conserved protein motifs with p < 0.05 and width 25–50 were found. Antigenic propensity-associated sequences were 20–29. Present study findings hint about the use of the bacterial GUS enzymes against breast cancer tumors after modifications via site-directed mutagenesis of catalytic sites involved in the activation of estrogens and through destabilization of these enzymes.
Coupled Large Deformation Finite Element Formulations for the Dynamics of Unsaturated Soil and Their Application
Authors: Ravichandran Nadarajah , Vickneswaran Tharshikka
Published: August 2022

Unsaturated soil is a three-phase medium with three interfaces, and the mathematical equations that represent its behavior must be developed in a fully coupled manner for accurately predicting its hydromechanical behavior. In this paper, a set of fully coupled governing equations was developed for the dynamics of unsaturated soil, considering the interaction among the bulk phases and interfaces. In addition to implementing the complete governing equations, a simplified formulation was developed for practical applications. The derivation of the finite element formulation considering all the terms in the partial differential equations resulted in a formulation called complete formulation and was solved for the first time in this paper. Another formulation called reduced formulation was derived by neglecting the relative accelerations and velocities of water and air in the governing equations. In addition, small and large deformation theories were developed and implemented for both formulations. To show the applicability of the proposed models, the dynamic behavior of an unsaturated soil embankment was simulated using both small and large deformation formulations by applying minor and severe earthquakes. The reduced formulation was found to be computationally efficient and numerically stable. The smaller displacements predicted by large deformation theories show that the results are consistent with the expected behavior. Large deformation theories are considered suitable when the geotechnical system undergoes large deformation and may lead to accurate prediction.
The Impact of Transoral Robotic Surgery on Erectile Dysfunction and Lower Urinary Tract Symptoms in Male Patients with Moderate-to-Severe Obstructive Sleep Apnea
Authors: Peng Chih-Kai , Tsao Chien-Han , Sung Wen-Wei , Wang Shao-Chuan , Chen Wen-Jung , Hsieh Tzuo-Yi , Yang Min-Hsin , Lee Tsung-Hsien , Chen Sung-Lang
Published: August 2022

Obstructive sleep apnea (OSA), lower urinary tract symptoms (LUTS), and erectile dysfunction (ED) are chronic conditions that seriously affect middle-aged men. This study aimed to evaluate the changes in the presence of these conditions after transoral robotic surgery (TORS) for OSA. This prospective observational study recruited 48 men with moderate-to-severe OSA (mean age 40.6 ± 8.1 years) who underwent TORS from October 2019 to November 2021 at a tertiary center. Baseline polysomnographic parameters, Epworth Sleepiness Scale (ESS), and demographic characteristics were measured. The evaluations of LUTS and ED were based on self-administered International Prostate Symptom Score (IPSS) and International Index of Erectile Function (IIEF-5) questionnaires, respectively, before TORS. The treatment outcomes were assessed three months postoperatively in the patients undergoing TORS due to moderate-to-severe OSA. There was significant Apnea-Hypopnea Index (AHI) reduction from 53.10 ± 25.77 to 31.66 ± 20.34 three months after undergoing TORS (p < 0.001). There was also a significant decrease in the total IPSS score (5.06 ± 5.42 at baseline to 2.98 ± 2.71 at three months postoperatively, p = 0.001), the storage domain, and the voiding domain (p < 0.05). The ED also improved significantly, as seen in the IIEF score (20.98 ± 3.32 to 22.17± 3.60, p = 0.007). The reduction of AHI was associated with changes in body weight and the lowest oxygen saturation (SpO2) levels during sleep (rho = 0.395, p = 0.005; rho = 0.526, p < 0.001, respectively). However, the reduction in AHI was not significantly associated with improvement in IPSS or IIEF scores (p > 0.05). For men with moderate-to-severe OSA, TORS can significantly improve the polysomnography parameters, sleep-related questionnaire scores, and quality of life, and alleviate ED and LUTS. AHI reduction is not a crucial factor for ED and LUTS improvement after TORS for OSA, especially in ED.
Interventions within the Scope of Occupational Therapy in the Hospital Discharge Process Post-Stroke: A Systematic Review
Authors: García-Pérez Patricia , Lara José Pablo , Rodríguez-Martínez María del Carmen , de la Cruz-Cosme Carlos
Published: August 2022

Stroke is one of the main causes of disability in adulthood. Its rehabilitation is a complex process that requires a multidisciplinary team of specialised professionals. The main goal of this review was to determine the impact of occupational therapy intervention post-stroke on the home discharge process. A systematic search was carried out of the following databases: Pubmed, Web of Science, PsycINFO, Scopus, Otseeker, and Dialnet. A screening was performed taking into account the type of article, including exclusively RCT, and type of intervention, only including interventions within the scope of occupational therapy that take place during the process of hospital discharge post-stroke. A total of 2285 citations were identified in the search from which 13 articles met the inclusion criteria. Comparisons of the groups indicated that early occupational therapy intervention during the process of hospital discharge can be effective in terms of functional recovery and can lead to the caregiver seeing improvements in self-efficacy and fatigue. In addition, the inclusion of a caregiver in the intervention influences the patient’s adherence to treatment, leading to a reduction in the cost of treatment and rehabilitation.