bluuebunny/crossref_metadata_2025 huggingface dataset:
HbA1c Screening for Diabetes in Patients with Acute Coronary Syndrome: A Worthwhile Test or a Pitfall?
Authors: Józwa Robert , Bryśkiewicz Marta , Safranow Krzysztof , Majkowska Liliana
Published: September 2021

Background: Diagnostic concordance between HbA1c and other glucose-based tests is imperfect, and data on this problem in acute coronary syndrome (ACS) are still lacking. The aim of this study was to identify undiagnosed glucose abnormalities in ACS patients, and to compare the effectiveness and consistency of the diagnostic screening based on HbA1c to the oral glucose tolerance test (OGTT). Methods: The study group consisted of 121 ACS patients, mean age 62.3 ± 11.6 years, without known glucose abnormalities. HbA1c, admission and fasting plasma glucose in the first days of hospitalization were assessed and referred to the results of OGTT performed two weeks after discharge. Results: OGTT identified normoglycemia in 45%, pre-diabetes in 39.4%, and diabetes in 15.6%, while HbA1c revealed these categories in 39.7%, 51.2%, and 9.1%, respectively. With an HbA1c cut-off ≥6.5% (48 mmol/mol) diagnostic for diabetes, the sensitivity of the method was 41%, while specificity was 98%, compared to the OGTT. The optimal HbA1c cut-off value at the crossing of sensitivity and specificity curves was 5.9%. The HbA1c value recommended for the diagnosis of pre-diabetes and optimal cut-off point were the same (5.7%). Conclusions: Using HbA1c without OGTT in an early but stable phase of ACS may result in a significant underdiagnosis of diabetes.
First Evidence of Contourite Drifts in the North-Western Sicilian Active Continental Margin (Southern Tyrrhenian Sea)
Authors: Spatola Daniele , Sulli Attilio , Casalbore Daniele , Chiocci Francesco Latino
Published: September 2021

We present the results of an integrated geomorphological and seismo-stratigraphic study based on high resolution marine data acquired in the north-western Sicilian continental margin. We document for the first time five contourite drifts (marked as EM1a, EM2b, EM2, EM3a, and EM3b), located in the continental slope at depths between ca. 400 and 1500 m. EM1a,b have been interpreted as elongated mounded drifts. EM1a,b are ca. 3 km long, 1.3 km wide, and have a maximum thickness of 36 m in their center that thins northwards, while EM1b is smaller with a thickness up to 24 m. They are internally characterized by mounded seismic packages dominated by continuous and parallel reflectors. EM2 is located in the upper slope at a depth of ca. 1470 m, and it is ca. 9.3 km long, more than 3.9 km wide, and has a maximum thickness of ca. 65 m. It consists of an internal aggradational stacking pattern with elongated mounded packages of continuous, moderate to high amplitude seismic reflectors. EM2 is internally composed by a mix of contourite deposits (Holocene) interbedded with turbiditic and/or mass flow deposits. EM1a,b and EM2 are deposited at the top of an erosional truncation aged at 11.5 ka, so they mostly formed during the Holocene. EM3a,b are ca. 16 km long, more than 6.7 km wide, and have a thickness up to 350 m. Both EM2 and EM3a,b have been interpreted as sheeted drift due to their morphology and seismic features. The spatial distribution of the contourite drifts suggests that the drifts are likely generated by the interaction of the LIW, and deep Tyrrhenian water (TDW) on the seafloor, playing an important role in the shaping this continental margin since the late Pleistocene-Holocene. The results may help to understand the deep oceanic processes affecting the north-western Sicilian continental margin.
Analysis of the Formation Mechanism and Evolution of the Perpendicular Cavitation Vortex of Tip Leakage Flow in an Axial-Flow Pump for Off-Design Conditions
Authors: Zhang Hu , Zang Jianbo , Shi Weidong , Zhang Desheng
Published: September 2021

To understand the formation mechanism and evolution process of the perpendicular cavitation vortex (PCV) of an axial flow pump for off-design conditions, turbulent cavitating flows were numerically investigated using the rotation curvature-corrected shear stress transport (SST-CC) turbulence model and the Zwart–Gerber–Belamri cavitation model. In this work, the origin and evolution of a PCV were analyzed through a high-speed photography experiment and numerical simulation. The results showed that the PCV came from a secondary tip leakage vortex (S-TLV) and was aggregated by the action of the re-entrant jet, combined with the cavitation bubbles driven by the radial flow to form the cavitation vortex (CV). With the joint action of leakage jet lifting and TLV entrainment, the PCV was reoriented and gradually became perpendicular to the chord direction. Then, the PCV and TLV collided, mixed, and entrained, which formed a strong pressure pulsation. The PCV was gradually divided into upper and lower parts. One part was combined with the residual part of the TLV and flowed to the next blade, and the other part flowed out of the impeller area along the axial direction. At the same time, the generation, evolution, and dissipation of the PCV formed high pulsation amplitudes and frequencies in the middle and rear above the blade suction.
Soil Fabric and Transitional Behavior in Completely Decomposed Granite: An Example of Well-Graded Soil
Authors: Elkamhawy Elsayed , Wang Huabin , Salem Tarek N. , Vranay František , Zelenakova Martina
Published: September 2021

Unlike sedimentary soils, limited studies have dealt with completely decomposed granite (CDG) soils, even though they are plentiful and used extensively in several engineering applications. In this paper, a set of triaxial compression tests have been conducted on well-graded intact and disturbed CDG soils to study the impact of the fabric on soil behavior. The soil behavior was robustly affected by the soil fabric and its mineral composition. The intact soil showed multiple parallel compression lines, while a unique isotropic compression line was present in the case of disturbed soil. Both the intact and disturbed soils showed unique critical state lines (CSL) in both the e-log p′ and q-p′ spaces. The intact soil showed behavior unlike other transitional soils that have both distinct isotropic compression lines ICLs and CSLs. The gradient of the unique ICL of the disturbed soil was much more than that of the parallel compression lines of the intact soil. In the intact soil, the slope of the unique CSL (M) in the q-p′ space was higher than that of the disturbed soil. The isotropic response was present for both the intact and disturbed soils after erasing the inherited anisotropy as the stress increased with irrecoverable volumetric change. Soil fabric is considered the dominant factor in the transitional behavior and such a mode of soil behavior is no longer restricted to gap-graded soil as previously thought.
Associations of the SREBF2 Gene and INSIG2 Polymorphisms with Obesity and Dyslipidemia in Thai Psychotic Disorder Patients Treated with Risperidone
Authors: Vanwong Natchaya , Sukasem Chonlaphat , Unaharassamee Weerapon , Jiratjintana Napa , Na Nakorn Chalitpon , Hongkaew Yaowaluck , Puangpetch Apichaya
Published: September 2021

Background: Patients with psychotic disorders who receive atypical antipsychotic drugs often develop metabolic abnormalities. The sterol regulatory element-binding factor 2 (SREBF2) gene and insulin-induced gene (INSIG) have important roles in lipid metabolism. A previous study indicated that risperidone stimulated both lipogenesis and cholesterogenesis through activation of SREBP2 expression and inhibition of INSIG2. The SREBF2 gene and INSIG2 polymorphisms have been reported to be associated with metabolic abnormalities. Objective: To investigate the association of the SREBF2 gene (rs1052717, rs2267439, and rs2267443) and INSIG2 (rs7566605, rs11123469, and rs17587100) polymorphisms and the presence of obesity and dyslipidemia in Thai psychotic disorder patients treated with risperidone. Methods: All 113 psychiatric patients using risperidone were evaluated for their lipid profile and screened for obesity criteria. We genotyped the SREBF2 gene and INSIG2 polymorphisms using TaqMan real-time polymerase chain reaction. Results: None of the studied SREBF2 gene and INSIG2 SNPs were associated with obesity in Thai psychotic disorder patients receiving risperidone. Nonetheless, the SREBF2 rs2267443 (G/A) A-allele carriers were at a higher risk for hypertriglyceridemia, whereas the INSIG2 rs11123469 (T/C) C-allele carriers had a lower risk for hypertriglyceridemia, after being adjusted for clinical characteristics using multiple logistic regression. Conclusions: Our findings suggest that the SREBF2 gene rs2267443 (G/A) and the INSIG2 rs11123469 (T/C) polymorphisms are associated with dyslipidemia in Thai psychotic disorder patients treated with risperidone. Further studies with prospective designs and larger patient groups are needed.
Systematic Search for Novel Circulating Biomarkers Associated with Extracellular Vesicles in Alzheimer’s Disease: Combining Literature Screening and Database Mining Approaches
Authors: Vogrinc David , Goričar Katja , Kunej Tanja , Dolžan Vita
Published: September 2021

miRNAs play an important role in neurodegenerative diseases. Many miRNA-target gene interactions (MTI) have been experimentally confirmed and associated with Alzheimer’s disease (AD). miRNAs may also be contained within extracellular vesicles (EVs), mediators of cellular communication and a potential source of circulating biomarkers in body fluids. Therefore, EV-associated miRNAs (EV-miRNAs) in peripheral blood could support earlier and less invasive AD diagnostics. We aimed to prioritize EV-related miRNA with AD-related genes and to identify the most promising candidates for novel AD biomarkers. A list of unique EV-miRNAs from the literature was combined with a known set of AD risk genes and enriched for MTI. Additionally, miRNAs associated with the AD phenotype were combined with all known target genes in MTI enrichment. Expression in different sample types was analyzed to identify AD-associated miRNAs with the greatest potential as AD circulating biomarkers. Four common MTI were observed between EV-miRNAs and AD-associated miRNAs: hsa-miR-375–APH1B, hsa-miR-107–CDC42SE2, hsa-miR-375–CELF2, and hsa-miR-107–IL6. An additional 61 out of 169 unique miRNAs (36.1%) and seven out of 84 unique MTI (8.3%), observed in the body fluids of AD patients, were proposed as very strong AD-circulating biomarker candidates. Our analysis summarized several potential novel AD biomarkers, but further studies are needed to evaluate their potential in clinical practice.
Low Preoperative Lymphocyte-to-Monocyte Ratio Is Predictive of the 5-Year Recurrence of Bladder Tumor after Transurethral Resection
Authors: Kim Kyungmi , Yu Jihion , Park Jun-Young , Baek Sungwoon , Hwang Jai-Hyun , Choi Woo-Jong , Kim Young-Kug
Published: September 2021

Many studies have investigated the prognostic significance of peripheral blood parameters—including lymphocyte-to-monocyte ratio (LMR)—in several cancers in recent decades. We evaluated the prognostic factors for five-year tumor recurrence after the transurethral resection of a bladder tumor (TURBT). In total, 151 patients with non-muscle invasive bladder tumors who underwent TURBT under spinal anesthesia were selected for this retrospective analysis. The time to tumor recurrence was determined by the number of days from surgery until there was a pathological confirmation of tumor recurrence. The preoperative and postoperative laboratory values were defined as results within one month prior to and one month after TURBT. Univariate and multivariate Cox regression analyses were performed. Seventy-one patients (47.0%) developed recurrent bladder tumors within five years after the first TURBT surgery. The multivariate Cox regression analysis revealed that preoperative LMR (hazard ratio, 0.839; 95% confidence interval, 0.739–0.952; p = 0.006) and multiple tumor sites (hazard ratio, 2.072; 95% confidence interval, 1.243–3.453; p = 0.005) were independent recurrence predictors in patients with recurrent bladder tumors within five years after the TURBT. A low preoperative LMR is an important predictor for the recurrence of a bladder tumor during a five-year follow-up period after surgery.
Customer Value Creation for the Emerging Market Middle Class: Perspectives from Case Studies in India
Authors: Sharmelly Rifat , Klarin Anton
Published: September 2021

This paper examines the customer value creation framework and discusses the design of the key elements for product development in emerging markets. A scientometric/bibliometric scoping literature review identifies a clear gap in the current research in studying prerequisites for customer value creation in emerging market contexts. Observing experiences of Daikin and Renault in the context of India, the purpose of this paper is to identify value creation strategic choices following which comprehensive customer value offerings in products and services can be successfully created by firms across the four facets of the framework in emerging markets. Value creation strategies include having a nuanced understanding of the latent contextual needs to offer localized high-quality products that embody distinct functional attributes that provide a functional value and being responsive to specific emotional needs and epistemic experiences of the target customers in product and service offerings to deliver a greater experiential value. Furthermore, the products should adopt a localized operational excellence strategy throughout the value chain to reduce costs for competitive price offerings in order to deliver superior cost value and develop brand image and equity strategy, thereby allowing for the provision of a greater symbolic value. Experiences of successful firms demonstrate the need for extensive local research into the emerging market followed by localization of production and development of a distribution network to be able to offer customized products at competitive prices whilst maintaining the brand value. We thus extend the customer value creation framework by introducing localization as a necessary condition for successful organizational performance in emerging markets.
One Good Turn Deserves Another: Antecedents of Online Karaoke Paid Gift-Sending from Social Exchange Perspectives
Authors: Jia Susan (Sixue) , Wu Banggang
Published: September 2021

Understanding the dynamics of online karaoke virtual gift sending helps maximize its utility for all participants, including viewers, broadcasters, and platforms. However, extant paid gift-sending studies lack an integrated theoretical explanation of its incentives as well as practical implications that can facilitate the quantifiable implementation of service improvement. This study has successfully uncovered the motivation of paid gift-sending in an online karaoke context from a social exchange perspective using social exchange theory. By observing the activities of 11,640 online karaoke users over one year, it was discovered that their gift-sending behaviors adhere to the patterns of more-follower-more-gift-sending and receive-more-send-more. Moreover, such patterns are more pronounced for collaborative users and are accentuated over time. Theoretically, this study extends the scope of social commerce studies from B2C to C2C scenarios with more complicated interpersonal dynamics. Meanwhile, managers are advised to encourage following, stimulate collaboration, inject additional virtual gifts into the “market”, and retain their customers to generate long-term profits.
Study of Early Onset Schizophrenia: Associations of GRIN2A and GRIN2B Polymorphisms
Authors: Poltavskaya Evgeniya G. , Fedorenko Olga Yu. , Kornetova Elena G. , Loonen Anton J. M. , Kornetov Alexander N. , Bokhan Nikolay A. , Ivanova Svetlana A.
Published: September 2021

Background: Schizophrenia is a complex mental disorder with a high heritability. Dysfunction of the N-methyl-D-aspartate (NMDA)-type glutamate receptors may be involved in the pathogenesis of schizophrenia. In this study, we examined the contribution of GRIN2A and GRIN2B (Glutamate Ionotropic Receptor NMDA Type Subunit 2A/2B) polymorphisms to the clinical features of schizophrenia, such as the leading symptoms, the type of course, and the age of onset. Methods: A population of 402 Russian patients with schizophrenia from the Siberian region was investigated. Genotyping of seventeen single-nucleotide polymorphisms (SNPs) in GRIN2A and GRIN2B was performed using QuantStudio™ 3D Digital PCR System Life Technologies amplifier using TaqMan Validated SNP Genotyping Assay kits (Applied Biosystems). The results were analyzed using Chi-square and the Fisher’s exact tests. Results: We found an association of GRIN2A rs7206256 and rs11644461 and GRIN2B rs7313149 with the early onset (before the age of 18 years old) schizophrenia. We did not reveal any associations of GRIN2A and GRIN2B polymorphisms with leading (positive vs. negative) symptoms or type of course (continuous vs. episodic) of schizophrenia. Conclusions: In the study, we confirmed the involvement of the GRIN2A and GRIN2B genes in the early onset of schizophrenia in a Russian population of the Siberian region.
Decoupled Multi-Loop Robust Control for a Walk-Assistance Robot Employing a Two-Wheeled Inverted Pendulum
Authors: Wang Fu-Cheng , Chen Yu-Hong , Wang Zih-Jia , Liu Chi-Hao , Lin Pei-Chun , Yen Jia-Yush
Published: September 2021

This paper develops a decoupled multi-loop control for a two-wheeled inverted pendulum (TWIP) robot that can assist user’s with walking. The TWIP robot is equipped with two wheels driven by electrical motors. We derive the system’s transfer function and design a robust loop-shaping controller to balance the system. The simulation and experimental results show that the TWIP system can be balanced but might experience velocity drifts because its balancing point is affected by model variations and disturbances. Therefore, we propose a multi-loop control layout consisting of a velocity loop and a position loop for the TWIP robot. The velocity loop can adjust the balancing point in real-time and regulate the forward velocity, while the position loop can achieve position tracking. For walking assistance, we design a decoupled control structure that transfers the linear and rotational motions of the robot to the commands of two parallel motors. We implement the designed controllers for simulation and experiments and show that the TWIP system employing the proposed decoupled multi-loop control can provide satisfactory responses when assisting with walking.
An Extension of the Brouwer–Zimmermann Algorithm for Calculating the Minimum Weight of a Linear Code
Authors: Bouyuklieva Stefka , Bouyukliev Iliya
Published: September 2021

A modification of the Brouwer–Zimmermann algorithm for calculating the minimum weight of a linear code over a finite field is presented. The aim was to reduce the number of codewords for consideration. The reduction is significant in cases where the length of a code is not divisible by its dimensions. The proposed algorithm can also be used to find all codewords of weight less than a given constant. The algorithm is implemented in the software package QextNewEdition.
Optimal Control Problems Involving Combined Fractional Operators with General Analytic Kernels
Authors: Ndaïrou Faïçal , Torres Delfim F. M.
Published: September 2021

Fractional optimal control problems via a wide class of fractional operators with a general analytic kernel are introduced. Necessary optimality conditions of Pontryagin type for the considered problem are obtained after proving a Gronwall type inequality as well as results on continuity and differentiability of perturbed trajectories. Moreover, a Mangasarian type sufficient global optimality condition for the general analytic kernel fractional optimal control problem is proved. An illustrative example is discussed.
Fatigue Performance of Thin Laser Butt Welds in HSLA Steel
Authors: Riofrío Patricio G. , Antunes Fernando , Ferreira José , Batista António Castanhola , Capela Carlos
Published: September 2021

This work is focused on understanding the significant factors affecting the fatigue strength of laser-welded butt joints in thin high-strength low-alloy (HSLA) steel. The effects of the weld profile, imperfections, hardness, and residual stresses were considered to explain the results found in the S-N curves of four welded series. The results showed acceptable fatigue strength although the welded series presented multiple-imperfections. The analysis of fatigue behavior at low stress levels through the stress-concentrating effect explained the influence of each factor on the S-N curves of the welded series. The fatigue limits of the welded series predicted through the stress-concentrating effect and by the relationship proposed by Murakami showed good agreement with the experimental results.
Ultra-Fine Centrifugal Concentration of Bastnaesite Ore
Authors: Norgren Alex , Anderson Corby
Published: September 2021

Historically, the ability to effectively separate carbonate gangue from bastnaesite via flotation has frequently proven to be challenging without sacrificing significant rare earth oxide (REO) grade or recovery. However, in light of the fact that the rare earth bearing minerals often exhibit higher specific gravities than the carbonate gangue, the possibility exists that the use of gravity separation could be used to achieve such a selective separation. This however is complicated by the fact that, in cases such as this study when the liberation size is finer than 50 µm, most traditional gravity separation methods become increasingly challenging. The purposes of this study is to determine the applicability of gravity concentrators to beneficiate bastnaesite from deleterious calcite bearing flotation feed material. Via the use of a UF Falcon, it was possible to achieve rougher gravity REO recoveries approaching the upper 80% range while rejecting on the order of 30% of the total calcium. In terms of purely REO recovery, this represents a significant improvement over results obtained via a traditional Falcon in previously reported studies.
Evaluating Different Storage Media for Identification of Taenia saginata Proglottids Using MALDI-TOF Mass Spectrometry
Authors: Wendel Tabea P. , Feucherolles Maureen , Rehner Jacqueline , Poppert Sven , Utzinger Jürg , Becker Sören L. , Sy Issa
Published: September 2021

Taenia saginata is a helminth that can cause taeniasis in humans and cysticercosis in cattle. A species-specific diagnosis and differentiation from related species (e.g., Taenia solium) is crucial for individual patient management and disease control programs. Diagnostic stool microscopy is limited by low sensitivity and does not allow discrimination between T. saginata and T. solium. Molecular diagnostic approaches are not routinely available outside research laboratories. Recently, matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) was proposed as a potentially suitable technique for species-specific helminth diagnosis. However, standardized protocols and commercial databases for parasite identification are currently unavailable, and pre-analytical factors have not yet been assessed. The purpose of this study was to employ MALDI-TOF MS for the identification of T. saginata proglottids obtained from a human patient, and to assess the effects of different sample storage media on the technique’s diagnostic accuracy. We generated T. saginata-specific main spectral profiles and added them to an in-house database for MALDI-TOF MS-based diagnosis of different helminths. Based on protein spectra, T. saginata proglottids could be successfully differentiated from other helminths, as well as bacteria and fungi. Additionally, we analyzed T. saginata proglottids stored in (i) LC–MS grade water; (ii) 0.45% sodium chloride; (iii) 70% ethanol; and (iv) 37% formalin after 2, 4, 6, 8, 12, and 24 weeks of storage. MALDI-TOF MS correctly identified 97.2–99.7% of samples stored in water, sodium chloride, and ethanol, with log-score values ≥2.5, thus indicating reliable species identification. In contrast, no protein spectra were obtained for samples stored in formalin. We conclude that MALDI-TOF-MS can be successfully employed for the identification of T. saginata, and that water, sodium chloride, and ethanol are equally effective storage solutions for prolonged periods of at least 24 weeks.
Serra da Estrela PDO Cheese Microbiome as Revealed by Next Generation Sequencing
Authors: Rocha Rui , Vaz Velho Manuela , Santos Joana , Fernandes Paulo
Published: September 2021

Serra da Estrela PDO cheese is the oldest traditional cheese manufactured in Portugal. In this work, its microbiome as well as the main raw materials used in cheese production, raw ewes’ milk and thistle flowers (Cynara cardunculus L.), were characterized using next generation sequencing. Samples were accordingly retrieved from a local producer over two consecutive production campaigns and at different time periods within each campaign. The bacterial and fungi communities associated with each matrix were accessed through sequencing of V3–V4 and Internal Transcribed Spacer 2 regions of rRNA gene amplicons, respectively. A high microbial diversity was found associated to each matrix, differing significantly (p < 0.05) from each other. Over 500 taxa were identified in each analyzed matrix, ranging from dominant (relative abundance > 1%), sub-dominant (0.01–1%) and rare taxa (<0.01%). Specifically, in cheese, 30 taxa were present in all analyzed samples (core taxa), including species of Leuconostoc spp. and Lactococcus spp. for bacteria and Candida spp., Debaryomyces spp. and Yarrowia spp. for fungi, that were cumulatively the most prevalent genera in Serra da Estrela PDO cheese (average relative abundance ≥10%). Ultimately, this characterization study may contribute to a better understanding of the microbial dynamics of this traditional PDO product, namely the influence of raw materials on cheese microbiome, and could assist producers interested in preserving the identity, quality and safety of Serra da Estrela PDO cheese.
Growth Mechanism of Micro/Nano Metal Dendrites and Cumulative Strategies for Countering Its Impacts in Metal Ion Batteries: A Review
Authors: Ramasubramanian Brindha , Reddy M. V. , Zaghib Karim , Armand Michel , Ramakrishna Seeram
Published: September 2021

Metal-ion batteries are capable of delivering high energy density with a longer lifespan. However, they are subject to several issues limiting their utilization. One critical impediment is the budding and extension of solid protuberances on the anodic surface, which hinders the cell functionalities. These protuberances expand continuously during the cyclic processes, extending through the separator sheath and leading to electrical shorting. The progression of a protrusion relies on a number of in situ and ex situ factors that can be evaluated theoretically through modeling or via laboratory experimentation. However, it is essential to identify the dynamics and mechanism of protrusion outgrowth. This review article explores recent advances in alleviating metal dendrites in battery systems, specifically alkali metals. In detail, we address the challenges associated with battery breakdown, including the underlying mechanism of dendrite generation and swelling. We discuss the feasible solutions to mitigate the dendrites, as well as their pros and cons, highlighting future research directions. It is of great importance to analyze dendrite suppression within a pragmatic framework with synergy in order to discover a unique solution to ensure the viability of present (Li) and future-generation batteries (Na and K) for commercial use.
Plastome Characterization and Phylogenomic Analysis Yield New Insights into the Evolutionary Relationships among the Species of the Subgenus Bryocles (Hosta; Asparagaceae) in East Asia
Authors: Yang JiYoung , Choi Mi-Jung , Kim Seon-Hee , Choi Hyeok-Jae , Kim Seung-Chul
Published: September 2021

The genus Hosta, which has a native distribution in temperate East Asia and a number of species ranging from 23 to 40, represents a taxonomically important and ornamentally popular plant. Despite its taxonomic and horticultural importance, the genus Hosta has remained taxonomically challenging owing to insufficient diagnostic features, continuous morphological variation, and the process of hybridization and introgression, making species circumscription and phylogenetic inference difficult. In this study, we sequenced 11 accessions of Hosta plastomes, including members of three geographically defined subgenera, Hosta, Bryocles, and Giboshi, determined the characteristics of plastomes, and inferred their phylogenetic relationships. We found highly conserved plastomes among the three subgenera, identified several mutation hotspots that can be used as barcodes, and revealed the patterns of codon usage bias and RNA editing sites. Five positively selected plastome genes (rbcL, rpoB, rpoC2, rpl16, and rpl20) were identified. Phylogenetic analysis suggested (1) the earliest divergence of subg. Hosta, (2) non-monophyly of subg. Bryocles and its two sections (Lamellatae and Stoloniferae), (3) a sister relationship between H. sieboldiana (subg. Giboshi) and H. ventricosa (subg. Bryocles), and (4) reciprocally monophyletic and divergent lineages of H. capitata in Korea and Japan, requiring further studies of their taxonomic distinction.
Understanding a Mechanistic Basis of ABA Involvement in Plant Adaptation to Soil Flooding: The Current Standing
Authors: Zhao Yancui , Zhang Wenying , Abou-Elwafa Salah Fatouh , Shabala Sergey , Xu Le
Published: September 2021

Soil flooding severely impairs agricultural crop production. Plants can cope with flooding conditions by embracing an orchestrated set of morphological adaptations and physiological adjustments that are regulated by the elaborated hormonal signaling network. The most prominent of these hormones is ethylene, which has been firmly established as a critical signal in flooding tolerance. ABA (abscisic acid) is also known as a “stress hormone” that modulates various responses to abiotic stresses; however, its role in flooding tolerance remains much less established. Here, we discuss the progress made in the elucidation of morphological adaptations regulated by ABA and its crosstalk with other phytohormones under flooding conditions in model plants and agriculturally important crops.
Investigation of Impacts on Printed Circuit Board Laminated Composites Caused by Surface Finish Application
Authors: Froš Denis , Dušek Karel , Veselý Petr
Published: September 2021

The purpose of this study was to compare the strength of the bond between resin and glass cloth for various composites (laminates) and its dependence on utilized soldering pad surface finishes. Moreover, the impact of surface finish application on the thermomechanical properties of the composites was evaluated. Three different laminates with various thermal endurances were included in the study. Soldering pads were covered with OSP and HASL surface finishes. The strength of the cohesion of the resin upper layer was examined utilizing a newly established method designed for pulling tests. Experiments studying the bond strength were performed at a selection of laminate temperatures. Changes in thermomechanical behavior were observed by thermomechanical and dynamic mechanical analyses. The results confirmed the influence of the type of laminate and used surface finish on bond strength. In particular, permanent polymer degradation caused by thermal shock during HASL application was observed in the least thermally resistant laminate. A response to thermal shock was detected in thermomechanical properties of other laminates as well, but it does not seem to be permanent.
Innovation in Aircraft Cabin Interior Panels Part I: Technical Assessment on Replacing the Honeycomb with Structural Foams and Evaluation of Optimal Curing of Prepreg Fiberglass
Authors: Franco-Urquiza Edgar Adrián , Dollinger Annika , Torres-Arellano Mauricio , Piedra Saúl , Alcántara Llanas Perla Itzel , Rentería-Rodríguez Victoria , Zarate Pérez Cecilia
Published: September 2021

Sandwich composites are widely used in the manufacture of aircraft cabin interior panels for commercial aircraft, mainly due to the light weight of the composites and their high strength-to-weight ratio. Panels are used for floors, ceilings, kitchen walls, cabinets, seats, and cabin dividers. The honeycomb core of the panels is a very light structure that provides high rigidity, which is considerably increased with fiberglass face sheets. The panels are manufactured using the compression molding process, where the honeycomb core is crushed up to the desired thickness. The crushed core breaks fiberglass face sheets and causes other damage, so the panel must be reworked. Some damage is associated with excessive build-up of resin in localized areas, incomplete curing of the pre-impregnated fiberglass during the manufacturing process, and excessive temperature or residence time during the compression molding. This work evaluates the feasibility of using rigid polyurethane foams as a substitute for the honeycomb core. The thermal and viscoelastic behavior of the cured prepreg fiberglass under different manufacturing conditions is studied. The first part of this work presents the influence of the manufacturing parameters and the feasibility of using rigid foams in manufacturing flat panels oriented to non-structural applications. The conclusion of the article describes the focus of future research.
Hyaluronan and Derivatives: An In Vitro Multilevel Assessment of Their Potential in Viscosupplementation
Authors: La Gatta Annalisa , Stellavato Antonietta , Vassallo Valentina , Di Meo Celeste , Toro Giuseppe , Iolascon Giovanni , Schiraldi Chiara
Published: September 2021

In this research work, viscosupplements based on linear, derivatized, crosslinked and complexed HA forms were extensively examined, providing data on the hydrodynamic parameters for the water-soluble-HA-fraction, rheology, sensitivity to enzymatic hydrolysis and capacity to modulate specific biomarkers’ expression in human pathological chondrocytes and synoviocytes. Soluble HA ranged from 0 to 32 mg/mL and from 150 to 1330 kDa MW. The rheological behavior spanned from purely elastic to viscoelastic, suggesting the diversity of the categories that are suitable for restoring specific/different features of the healthy synovial fluid. The rheological parameters were reduced in a diverse manner upon dilution and hyaluronidases action, indicating different durations of the viscosupplementation effect. Bioactivity was found for all the samples, increasing the expression of different matrix markers (e.g., hyaluronan-synthase); however, the hybrid cooperative complexes performed better in most of the experiments. Hybrid cooperative complexes improved COLII mRNA expression (~12-fold increase vs. CTR), proved the most effective at preserving cell phenotype. In addition, in these models, the HA samples reduced inflammation. IL-6 was down-regulated vs. CTR by linear and chemically modified HA, and especially by hybrid complexes. The results represent the first comprehensive panel of data directly comparing the diverse HA forms for intra-articular injections and provide valuable information for tailoring products’ clinical use as well as for designing new, highly performing HA-formulations that can address specific needs.
Relaxation Phenomena in Chitosan-Au Nanoparticle Thin Films
Authors: Strupiechonski Elodie , Moreno-Ríos Marisa , Ávila-Dávila Erika O. , Román-Doval Ramón , Prokhorov Evgeny , Kovalenko Yuriy , Zárate-Triviño Diana G. , Medina Dora I. , Luna-Barcenas Gabriel
Published: September 2021

Chitosan–gold nanoparticle (CS/AuNP) thin films were synthesized through the chemical reduction of HAuCl4 in sodium citrate/chitosan solutions. The dielectric and dynamic mechanical behaviors of CS/AuNP films have been investigated as a function of moisture and HAuCl4 content. Two relaxation processes in the nanocomposites have been observed. The α-relaxation process is related to a glass transition in wet CS/AuNP films. However, in dry composites (with 0.2 wt% of moisture content), the glass transition vanished. A second relaxation process was observed from 70 °C to the onset of thermal degradation (160 °C) in wet films and from 33 °C to the onset of degradation in dry films. This relaxation is identified as the σ-relaxation and may be related to the local diffusion process of ions between high potential barriers in disordered systems. The α- and σ-relaxation processes are affected by the HAuCl4 content of the solutions from which films were obtained because of the interaction between CS, sodium succinate, and gold nanoparticles. With about 0.6 mM of HAuCl4, the conductivity of both wet and dry films sharply increased by six orders, corresponding to the percolation effect, which may be related to the appearance of a conductivity pathway between AuNPs, HAuCl4, and NaCl.
Bioactive Peptides from Liquid Milk Protein Concentrate by Sequential Tryptic and Microbial Hydrolysis
Authors: Nath Arijit , Csighy Attila , Eren Burak Attila , Tjandra Nugraha David , Pásztorné-Huszár Klára , Tóth Attila , Takács Krisztina , Szerdahelyi Emőke , Kiskó Gabriella , Kovács Zoltán , Koris András , Vatai Gyula
Published: September 2021

Recently, bioactive peptides as a health-promoting agent have come to the forefront of health research; however, industrial production is limited, possibly due to the lack of the required technological knowledge. The objective of the investigation was to prepare bioactive peptides with hypoallergenic properties from liquid milk protein concentrate (LMPC), through sequential enzymatic and microbial hydrolysis. LMPC was produced from ultra-heat-treated (UHT) skimmed cow’s milk using a nanofiltration membrane. The effect of the concentration of trypsin (0.008–0.032 g·L−1) on the hydrolysis of LMPC was studied. Subsequently, the hydrolysis of tryptic-hydrolyzed LMPC (LMPC-T) with lactic acid bacteria was performed, and the effect of glucose in microbial hydrolysis was studied. Aquaphotomic analysis of the hydrolysis of LMPC was performed using the spectral range of 1300–1600 nm (near-infrared spectra). Changes in antioxidant capacity, anti-angiotensin-converting enzyme activity, and antibacterial activity against Bacillus cereus, Staphylococcus aureus and Listeria monocytogenes were noted after the sequential tryptic and microbial hydrolysis of LMPC. Allergenicity in LMPC was reduced, due to sequential hydrolysis with 0.016 g·L−1 of trypsin and lacteal acid bacteria. According to the aquaphotomic analysis result, there was a dissociation of hydrogen bonds in compounds during the initial period of fermentation and, subsequently, the formation of compounds with hydrogen bonds. The formation of compounds with a hydrogen bond was more noticeable when microbial hydrolysis was performed with glucose. This may support the belief that the results of the present investigation will be useful to scale up the process in the food and biopharmaceutical industries.
Smooth Stitching Method for the Texture Seams of Remote Sensing Images Based on Gradient Structure Information
Authors: Deng Danjun
Published: September 2021

Traditional smooth stitching method for the texture seams of remote sensing images is affected by gradient structure information, leading to poor stitching effect. Therefore, a smooth stitching method for the texture seams of remote sensing images based on gradient structure information is proposed in this research. By matching the feature points of remote sensing images and introducing a block link constraint and shape distortion constraint, the modified stitching image is obtained. By using remote sensing image fusion, the smooth stitching image of texture seams is obtained, and the local overlapping area of the texture is optimized. The main direction of texture seams is determined by calculating the gradient structure information of texture seams in horizontal and vertical directions. By selecting the initial point, the optimal stitching line is extracted by using the minimum mean value of the cumulative error of the smooth stitching line. By using the method of boundary correlation constraints, matching the feature points of the texture seams of remote sensing images and selecting the best matching pair, a smooth stitching algorithm for the texture seams of remote sensing image is designed, which realizes the smooth stitching of the texture seams of remote sensing images. Experimental results show that the design method has good performance in stitching accuracy and efficiency in the smooth stitching of the texture seams of remote sensing images. Specifically, the Liu et al. and the Zhang et al. methods that are the benchmark studies in the literature are introduced as a comparison, and the stitching experiment is carried out. The test is carried out according to accuracy and time and the proposed method achieves better results by almost 25%.
Sustainable Application of Biosorption and Bioaccumulation of Persistent Pollutants in Wastewater Treatment: Current Practice
Authors: Filote Cătălina , Roșca Mihaela , Hlihor Raluca , Cozma Petronela , Simion Isabela , Apostol Maria , Gavrilescu Maria
Published: September 2021

Persistent toxic substances including persistent organic pollutants and heavy metals have been released in high quantities in surface waters by industrial activities. Their presence in environmental compartments is causing harmful effects both on the environment and human health. It was shown that their removal from wastewaters using conventional methods and adsorbents is not always a sustainable process. In this circumstance, the use of microorganisms for pollutants uptake can be seen as being an environmentally-friendly and cost-effective strategy for the treatment of industrial effluents. However, in spite of their confirmed potential in the remediation of persistent pollutants, microorganisms are not yet applied at industrial scale. Thus, the current paper aims to synthesize and analyze the available data from literature to support the upscaling of microbial-based biosorption and bioaccumulation processes. The industrial sources of persistent pollutants, the microbial mechanisms for pollutant uptake and the significant results revealed so far in the scientific literature are identified and covered in this review. Moreover, the influence of different parameters affecting the performance of the discussed systems and also very important in designing of treatment processes are highly considered. The analysis performed in the paper offers an important perspective in making decisions for scaling-up and efficient operation, from the life cycle assessment point of view of wastewater microbial bioremediation. This is significant since the sustainability of the microbial-based remediation processes through standardized methodologies such as life cycle analysis (LCA), hasn’t been analyzed yet in the scientific literature.
Implant Fracture: A Narrative Literature Review
Authors: Tallarico Marco , Meloni Silvio Mario , Park Chang-Joo , Zadrożny Łukasz , Scrascia Roberto , Cicciù Marco
Published: September 2021

Implants fracture is a rare but possible complication that leads to implants failure after prostheses delivery. Mechanical properties play a key role in the failure of dental implant systems. The aim of this narrative review was to evaluate the existing evidence in identifying etiology risk factors for implants fracture. The focused question was to evaluate whether there was any possible factors influencing the fracture of dental implants. A literature search of papers written in English, published from 1967 to July 2021, and reporting incidence of implants fracture in human with at least 15 participants (and one year of follow-up) was conducted using PubMed database including MeSH and free text terms and filters. Selected manuscripts were analyzed and discussed. The outcomes were the incidence of implants failure due to a fracture and the associated risk factors. A total of 96 articles were initially selected, but only eight articles were included according to the search criteria (two systematic reviews and six retrospective evaluation). Incidence of implants fracture ranged from 0.2 to 2.3%, with a mean value of 0.52%. Poor implant planning, including implant design and diameter, and occlusal overloading, were the most common variables associated with implants fracture. Implant removal is the only possible treatment and hence prevention, including stability of the marginal bone loss, is mandatory.
Global Contexts: How Countries Shape the COVID-19 Experience of Amish and Mennonite Missionaries Abroad
Authors: Corcoran Katie E. , Stein Rachel E. , Colyer Corey J. , Mackay Annette M. , Guthrie Sara K.
Published: September 2021

Across the globe, governments restricted social life to slow the spread of COVID-19. Several conservative Protestant sects resisted these policies in the United States. We do not yet know if theology shaped the resistance or if it was more a product of a polarized national political context. We argue that the country context likely shapes how conservative Protestants’ moral worldview affects their perceptions of the pandemic and government restrictions. Countries implementing more regulations, those with limited access to healthcare, food, and other essential services, and those with past histories of epidemics may all shape residents’ perceptions. Drawing on the case of American Amish and Mennonite missionaries stationed abroad, we content-analyzed accounts of the pandemic from an international Amish and Mennonite correspondence newspaper. We found that the missionaries’ perceptions of the pandemic and governmental restrictions differ from those of their U.S. counterparts, which suggests that context likely shapes how religious moral worldviews express themselves concerning public health interventions.
Evaluation of Three Gridded Precipitation Products to Quantify Water Inputs over Complex Mountainous Terrain of Western China
Authors: Zhang Liping , Lan Ping , Qin Guanghua , Mello Carlos R. , Boyer Elizabeth W. , Luo Pingping , Guo Li
Published: September 2021

This study evaluates the capacity of three gridded precipitation products (MSWEP V2.2, TRMM-3B42 V7, and GPM-IMERG V6) to detect precipitation in the Min Jiang watershed, a data-scarce and mountainous region in western China. A set of statistical and contingency indices is calculated for the precipitation products and compared with rain gauge observations at 23 ground stations from July 2000 to May 2016. Consistency between gridded and ground precipitation datasets is examined at different temporal (i.e., daily, monthly, seasonally, and annually) and spatial (i.e., site level, sub-regional level, and watershed level) resolutions. We identify possible reasons for discrepancies among precipitation datasets. Our results indicate that: (1) the MSWEP product is best suited for the study of long-term mesoscale rainfall, rather than short-term light or extreme rainfall; (2) the IMERG product represents stable performance for the simulation of rainfall spatial variability and detection capability; and (3) Composition of the datasets, climatic systems, and regional topography are key factors influencing the consistency between gridded and ground precipitation datasets. Therefore, we suggest using MSWEP V2.2 and GPM-IMERG V6 as potential precipitation data sources for hydrometeorological studies over the Min Jiang watershed. The findings of this study inform future hydrometeorological and climate applications in data-scarce regions with complex terrain.