bluuebunny/crossref_metadata_2025 huggingface dataset:
A Multi-Task Decomposition-Based Evolutionary Algorithm for Tackling High-Dimensional Bi-Objective Feature Selection
Authors: Xu Hang , Huang Chaohui , Lin Jianbing , Lin Min , Zhang Huahui , Xu Rongbin
Published: April 2024

Evolutionary algorithms have been widely applied for solving multi-objective optimization problems, while the feature selection in classification can also be treated as a discrete bi-objective optimization problem if attempting to minimize both the classification error and the ratio of selected features. However, traditional multi-objective evolutionary algorithms (MOEAs) may have drawbacks for tackling large-scale feature selection, due to the curse of dimensionality in the decision space. Therefore, in this paper, we concentrated on designing an multi-task decomposition-based evolutionary algorithm (abbreviated as MTDEA), especially for handling high-dimensional bi-objective feature selection in classification. To be more specific, multiple subpopulations related to different evolutionary tasks are separately initialized and then adaptively merged into a single integrated population during the evolution. Moreover, the ideal points for these multi-task subpopulations are dynamically adjusted every generation, in order to achieve different search preferences and evolutionary directions. In the experiments, the proposed MTDEA was compared with seven state-of-the-art MOEAs on 20 high-dimensional classification datasets in terms of three performance indicators, along with using comprehensive Wilcoxon and Friedman tests. It was found that the MTDEA performed the best on most datasets, with a significantly better search ability and promising efficiency.
f-Biharmonic Submanifolds in Space Forms and f-Biharmonic Riemannian Submersions from 3-Manifolds
Authors: Wang Ze-Ping , Qin Li-Hua
Published: April 2024

f-biharmonic maps are generalizations of harmonic maps and biharmonic maps. In this paper, we give some descriptions of f-biharmonic curves in a space form. We also obtain a complete classification of proper f-biharmonic isometric immersions of a developable surface in R3 by proving that a proper f-biharmonic developable surface exists only in the case where the surface is a cylinder. Based on this, we show that a proper biharmonic conformal immersion of a developable surface into R3 exists only in the case when the surface is a cylinder. Riemannian submersions can be viewed as a dual notion of isometric immersions (i.e., submanifolds). We also study f-biharmonicity of Riemannian submersions from 3-manifolds by using the integrability data. Examples are given of proper f-biharmonic Riemannian submersions and f-biharmonic surfaces and curves.
McmIQA: Multi-Module Collaborative Model for No-Reference Image Quality Assessment
Authors: Miao Han , Sang Qingbing
Published: April 2024

No reference image quality assessment is a technique that uses computers to simulate the human visual system and automatically evaluate the perceived quality of images. In recent years, with the widespread success of deep learning in the field of computer vision, many end-to-end image quality assessment algorithms based on deep learning have emerged. However, unlike other computer vision tasks that focus on image content, an excellent image quality assessment model should simultaneously consider distortions in the image and comprehensively evaluate their relationships. Motivated by this, we propose a Multi-module Collaborative Model for Image Quality Assessment (McmIQA). The image quality assessment is divided into three subtasks: distortion perception, content recognition, and correlation mapping. And specific modules are constructed for each subtask: the distortion perception module, the content recognition module, and the correlation mapping module. Specifically, we apply two contrastive learning frameworks on two constructed datasets to train the distortion perception module and the content recognition module to extract two types of features from the image. Subsequently, using these extracted features as input, we employ a ranking loss to train the correlation mapping module to predict image quality on image quality assessment datasets. Extensive experiments conducted on seven relevant datasets demonstrated that the proposed method achieves state-of-the-art performance in both synthetic distortion and natural distortion image quality assessment tasks.
Efficacy of Lidocaine Spray for Pain Reduction during Colposcopy-Directed Cervical Biopsies: A Randomized Controlled Trial
Authors: Mongkolmafai Ornwitsanate , Jeerakornpassawat Dhammapoj , Tantipalakorn Charuwan , Charoenkwan Kittipat , Suprasert Prapaporn , Srisomboon Jatupol , Tongsong Theera
Published: April 2024

Objectives: The objective of this study was to evaluate the efficacy of lidocaine spray in reducing the pain during colposcopy-directed cervical biopsy (CDB). Methods: From December 2017 to February 2019, 312 women undergoing CDBs were enrolled. The participants were randomized to three groups: group 1 (lidocaine spray), in which lidocaine spray was applied thoroughly to the cervix; group 2 (placebo), in which normal saline was applied thoroughly to the cervix; and group 3 (control), in which no anesthetic agent was applied to the cervix. Each woman completed a 10 cm visual analog scale to classify the subjective pain experience at three time points: baseline, immediately after biopsy, and 10 min after the procedure. The primary outcome of this study was the biopsy pain score. Results: The 312 enrolled women were randomly assigned to the three groups, amounting to 104 women per group. The clinical and pathological characteristics of the participants in all groups were comparable. The baseline, the biopsy, and the post-procedure pain scores were comparable among the three groups. There was a significant increase in the pain score from baseline to biopsy and from baseline to post-procedure in each group. The pain-score changes from baseline to biopsy in the lidocaine spray group significantly decreased when compared with the normal saline group (<0.001), and tended to decrease, though not significantly (p = 0.06), when compared with the control group. No complication with the intervention was observed. Conclusions: The application of lidocaine spray to the cervix has the benefit of reducing the pain associated with CDBs by a small amount. However, the intervention is safe and may be considered in nulliparous and/or overly anxious women undergoing the procedure.
Systematic Review and Meta-Analysis on Optimal Timing of Surgery for Acute Symptomatic Metastatic Spinal Cord Compression
Authors: Bresolin Nicola , Sartori Luca , Drago Giacomo , Pastorello Giulia , Gallinaro Paolo , Del Verme Jacopo , Zanata Roberto , Giordan Enrico
Published: April 2024

Introduction: Symptomatic acute metastatic spinal epidural cord compression (MSCC) is an emergency that requires multimodal attention. However, there is no clear consensus on the appropriate timing for surgery. Therefore, to address this issue, we conducted a systematic review and meta-analysis of the literature to evaluate the outcomes of different surgery timings. Methods: We searched multiple databases for studies involving adult patients suffering from symptomatic MSCC who underwent decompression with or without fixation. We analyzed the data by stratifying them based on timing as emergent (≤24 h vs. >24 h) and urgent (≤48 h vs. >48 h). The analysis also considered adverse postoperative medical and surgical events. The rates of improved outcomes and adverse events were pooled through a random-effects meta-analysis. Results: We analyzed seven studies involving 538 patients and discovered that 83.0% (95% CI 59.0–98.2%) of those who underwent urgent decompression showed an improvement of ≥1 point in strength scores. Adverse events were reported in 21% (95% CI 1.8–51.4%) of cases. Patients who underwent emergent surgery had a 41.3% (95% CI 20.4–63.3%) improvement rate but a complication rate of 25.5% (95% CI 15.9–36.3%). Patients who underwent surgery after 48 h showed 36.8% (95% CI 12.2–65.4%) and 28.6% (95% CI 19.5–38.8%) complication rates, respectively. Conclusion: Our study highlights that a 48 h window may be the safest and most beneficial for patients presenting with acute MSCC and a life expectancy of over three months.
The Increased Dissolution and Oral Absorption of Itraconazole by Nanocrystals with an Endogenous Small-Molecule Surfactant as a Stabilizer
Authors: Chang Sheng , Yang Qiang , Liu Jiahuan , Yin Li , Han Jihong , Zong Lanlan , Pu Xiaohui
Published: April 2024

The aim of this study was to develop cholic-acid-stabilized itraconazole nanosuspensions (ITZ-Nanos) with the objective of enhancing drug dissolution and oral absorption. A laboratory-scale microprecipitation–high-pressure homogenization method was employed for the preparation of the ITZ-Nanos, while dynamic light scattering, transmission electron microscope analysis, X-ray diffraction, differential scanning calorimetry, and high-performance liquid chromatography analysis were utilized to evaluate their physicochemical properties. The absorption and bioavailability of the ITZ-Nanos were assessed using Caco-2 cells and rats, with Sporanox® pellets as a comparison. Prior to lyophilization, the particle size of the ITZ-Nanos measured approximately 225.7 nm. Both X-ray diffraction and differential scanning calorimetry confirmed that the ITZ remained crystalline within the nanocrystals. Compared to the pellets, the ITZ-Nanos exhibited significantly higher levels of supersaturation dissolution and demonstrated enhanced drug uptake by the Caco-2 cells. The AUC(0–t) value for the ITZ-Nanos in rats was 1.33-fold higher than that observed for the pellets. These findings suggest that cholic acid holds promise as a stabilizer for ITZ nanocrystals, as well as potentially other nanocrystals.
Substitution Effects in Aryl Halides and Amides into the Reaction Mechanism of Ullmann-Type Coupling Reactions
Authors: Durán Rocío , Barrales-Martínez César , Santana-Romo Fabián , Rodríguez Diego F. , Zacconi Flavia C. , Herrera Barbara
Published: April 2024

In this article, we present a comprehensive computational investigation into the reaction mechanism of N-arylation of substituted aryl halides through Ullmann-type coupling reactions. Our computational findings, obtained through DFT ωB97X-D/6-311G(d,p) and ωB97X-D/LanL2DZ calculations, reveal a direct relation between the previously reported experimental reaction yields and the activation energy of haloarene activation, which constitutes the rate-limiting step in the overall coupling process. A detailed analysis of the reaction mechanism employing the Activation Strain Model indicates that the strain in the substituted iodoanilines is the primary contributor to the energy barrier, representing an average of 80% of the total strain energy. Additional analysis based on conceptual Density Functional Theory (DFT) suggests that the nucleophilicity of the nitrogen in the lactam is directly linked to the activation energies. These results provide valuable insights into the factors influencing energetic barriers and, consequently, reaction yields. These insights enable the rational modification of reactants to optimize the N-arylation process.
Exploring the Antiproliferative and Modulatory Effects of 1-Methoxyisobrassinin on Ovarian Cancer Cells: Insights into Cell Cycle Regulation, Apoptosis, Autophagy, and Its Interactions with NAC
Authors: Zigová Martina , Miškufová Viktória , Budovská Marianna , Michalková Radka , Mojžiš Ján
Published: April 2024

Ovarian cancer, a highly lethal malignancy among reproductive organ cancers, poses a significant challenge with its high mortality rate, particularly in advanced-stage cases resistant to platinum-based chemotherapy. This study explores the potential therapeutic efficacy of 1-methoxyisobrassinin (MB-591), a derivative of indole phytoalexins found in Cruciferae family plants, on both cisplatin-sensitive (A2780) and cisplatin-resistant ovarian cancer cells (A2780 cis). The findings reveal that MB-591 exhibits an antiproliferative effect on both cell lines, with significantly increased potency against cisplatin-sensitive cells. The substance induces alterations in the distribution of the cell cycle, particularly in the S and G2/M phases, accompanied by changes in key regulatory proteins. Moreover, MB-591 triggers apoptosis in both cell lines, involving caspase-9 cleavage, PARP cleavage induction, and DNA damage, accompanied by the generation of reactive oxygen species (ROS) and mitochondrial dysfunction. Notably, the substance selectively induces autophagy in cisplatin-resistant cells, suggesting potential targeted therapeutic applications. The study further explores the interplay between MB-591 and antioxidant N-acetylcysteine (NAC), in modulating cellular processes. NAC demonstrates a protective effect against MB-591-induced cytotoxicity, affecting cell cycle distribution and apoptosis-related proteins. Additionally, NAC exhibits inhibitory effects on autophagy initiation in cisplatin-resistant cells, suggesting its potential role in overcoming resistance mechanisms.
The Development of Aptamer-Based Gold Nanoparticle Lateral Flow Test Strips for the Detection of SARS-CoV-2 S Proteins on the Surface of Cold-Chain Food Packaging
Authors: Li Xiaotong , Wang Jiachen , Yang Ge , Fang Xiaona , Zhao Lianhui , Luo Zhaofeng , Dong Yiyang
Published: April 2024

The COVID-19 pandemic over recent years has shown a great need for the rapid, low-cost, and on-site detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). In this study, an aptamer-based colloidal gold nanoparticle lateral flow test strip was well developed to realize the visual detection of wild-type SARS-CoV-2 spike proteins (SPs) and multiple variants. Under the optimal reaction conditions, a low detection limit of SARS-CoV-2 S proteins of 0.68 nM was acquired, and the actual detection recovery was 83.3% to 108.8% for real-world samples. This suggests a potential tool for the prompt detection of SARS-CoV-2 with good sensitivity and accuracy, and a new method for the development of alternative antibody test strips for the detection of other viral targets.
Gas Chromatography–Mass Spectrometry Chemical Profiling of Commiphora myrrha Resin Extracts and Evaluation of Larvicidal, Antioxidant, and Cytotoxic Activities
Authors: Alanazi Naimah Asid H. , Alamri Abdullah A. , Mashlawi Abadi M. , Almuzaini Nujud , Mohamed Gamal , Salama Salama A.
Published: April 2024

Plant extracts and essential oils can be alternative environmentally friendly agents to combat pathogenic microbes and malaria vectors. Myrrh is an aromatic oligum resin that is extracted from the stem of Commiphora spp. It is used in medicine as an insecticide, cytotoxic, and aromatic. The current study assessed the effect of Commiphora myrrha resin extracts on the biological potency of the third larval stage of Aedes aegypti, as well as its antioxidant and cytotoxic properties against two types of tumor cells (HepG-2 and Hela cell lines). It also used GC–MS to determine the chemical composition of the C. myrrha resin extracts. Fifty components from the extracted plant were tentatively identified using the GC–MS method, with curzerene (33.57%) typically listed as the primary ingredient, but other compounds also make up a significant portion of the mixture, including 1-Methoxy-3,4,5,7-tetramethylnaphthalene (15.50%), β-Elemene (5.80%), 2-Methoxyfuranodiene (5.42%), 2-Isopropyl-4,7-Dimethyl-1-Naphthol (4.71%), and germacrene B (4.35%). The resin extracts obtained from C. myrrha exhibited significant efficacy in DPPH antioxidant activity, as evidenced by an IC50 value of 26.86 mg/L and a radical scavenging activity percentage of 75.06%. The 50% methanol extract derived from C. myrrha resins exhibited heightened potential for anticancer activity. It demonstrated substantial cytotoxicity against HepG-2 and Hela cells, with IC50 values of 39.73 and 29.41 µg mL−1, respectively. Notably, the extract showed non-cytotoxic activity against WI-38 normal cells, with an IC50 value exceeding 100 µg mL−1. Moreover, the selectivity index for HepG-2 cancer cells (2.52) was lower compared to Hela cancer cells (3.40). Additionally, MeOH resin extracts were more efficient against the different growth stages of the mosquito A. aegypti, with lower LC50, LC90, and LC95 values of 251.83, 923.76, and 1293.35 mg/L, respectively. In comparison to untreated groups (1454 eggs/10 females), the average daily number of eggs deposited (424 eggs/L) decreases at higher doses (1000 mg/L). Finally, we advise continued study into the possible use of C. myrrha resins against additional pests that have medical and veterinary value, and novel chemicals from this extract should be isolated and purified for use in medicines.
Photoinduced Site-Selective Aryl C-H Borylation with Electron-Donor-Acceptor Complex Derived from B2Pin2 and Isoquinoline
Authors: Li Manhong , Deng Yi-Hui , Chang Qianqian , Li Jinyuan , Wang Chao , Wang Leifeng , Sun Tian-Yu
Published: April 2024

Due to boron’s metalloid properties, aromatic boron reagents are prevalent synthetic intermediates. The direct borylation of aryl C-H bonds for producing aromatic boron compounds offers an appealing, one-step solution. Despite significant advances in this field, achieving regioselective aryl C-H bond borylation using simple and readily available starting materials still remains a challenge. In this work, we attempted to enhance the reactivity of the electron-donor-acceptor (EDA) complex by selecting different bases to replace the organic base (NEt3) used in our previous research. To our delight, when using NH4HCO3 as the base, we have achieved a mild visible-light-mediated aromatic C-H bond borylation reaction with exceptional regioselectivity (rr > 40:1 to single isomers). Compared with our previous borylation methodologies, this protocol provides a more efficient and broader scope for aryl C-H bond borylation through the use of N-Bromosuccinimide. The protocol’s good functional-group tolerance and excellent regioselectivity enable the functionalization of a variety of biologically relevant compounds and novel cascade transformations. Mechanistic experiments and theoretical calculations conducted in this study have indicated that, for certain arenes, the aryl C-H bond borylation might proceed through a new reaction mechanism, which involves the formation of a novel transient EDA complex.
BiMPADR: A Deep Learning Framework for Predicting Adverse Drug Reactions in New Drugs
Authors: Li Shuang , Zhang Liuchao , Wang Liuying , Ji Jianxin , He Jia , Zheng Xiaohan , Cao Lei , Li Kang
Published: April 2024

Detecting the unintended adverse reactions of drugs (ADRs) is a crucial concern in pharmacological research. The experimental validation of drug–ADR associations often entails expensive and time-consuming investigations. Thus, a computational model to predict ADRs from known associations is essential for enhanced efficiency and cost-effectiveness. Here, we propose BiMPADR, a novel model that integrates drug gene expression into adverse reaction features using a message passing neural network on a bipartite graph of drugs and adverse reactions, leveraging publicly available data. By combining the computed adverse reaction features with the structural fingerprints of drugs, we predict the association between drugs and adverse reactions. Our models obtained high AUC (area under the receiver operating characteristic curve) values ranging from 0.861 to 0.907 in an external drug validation dataset under differential experiment conditions. The case study on multiple BET inhibitors also demonstrated the high accuracy of our predictions, and our model’s exploration of potential adverse reactions for HWD-870 has contributed to its research and development for market approval. In summary, our method would provide a promising tool for ADR prediction and drug safety assessment in drug discovery and development.
ZnO-CeO2 Hollow Nanospheres for Selective Determination of Dopamine and Uric Acid
Authors: Zhang Yaru , Yan Xiaoxia , Chen Yifan , Deng Dongmei , He Haibo , Lei Yunyi , Luo Liqiang
Published: April 2024

ZnO-CeO2 hollow nanospheres have been successfully synthesized via the hard templating method, in which CeO2 is used as the support skeleton to avoid ZnO agglomeration. The synthesized ZnO-CeO2 hollow nanospheres possess a large electrochemically active area and high electron transfer owing to the high specific surface area and synergistic effect of ZnO and CeO2. Due to the above advantages, the resulting ZnO-CeO2 hollow spheres display high sensitivities of 1122.86 μA mM−1 cm−2 and 908.53 μA mM−1 cm−2 under a neutral environment for the selective detection of dopamine and uric acid. The constructed electrochemical sensor shows excellent selectivity, stability and recovery for the selective analysis of dopamine and uric acid in actual samples. This study provides a valuable strategy for the synthesis of ZnO-CeO2 hollow nanospheres via the hard templating method as electrocatalysts for the selective detection of dopamine and uric acid.
Financial Toxicity in Renal Patients (FINTORE) Study: A Cross-Sectional Italian Study on Financial Burden in Kidney Disease—A Project Protocol
Authors: Siligato Rossella , Gembillo Guido , Di Simone Emanuele , Di Maria Alessio , Nicoletti Simone , Scichilone Laura Maria , Capone Matteo , Vinci Francesca Maria , Bondanelli Marta , Malaventura Cristina , Storari Alda , Santoro Domenico , Di Muzio Marco , Dionisi Sara , Fabbian Fabio
Published: April 2024

Financial toxicity (FT) refers to the negative impact of health-care costs on clinical conditions. In general, social determinants of health, especially poverty, socioenvironmental stressors, and psychological factors, are increasingly recognized as important determinants of non-communicable diseases, such as chronic kidney disease (CKD), and their consequences. We aim to investigate the prevalence of FT in patients at different stages of CKD treated in our universal health-care system and from pediatric nephrology, hemodialysis, peritoneal dialysis and renal transplantation clinics. FT will be assessed with the Patient-Reported Outcome for Fighting Financial Toxicity (PROFFIT) score, which was first developed by Italian oncologists. Our local ethics committee has approved the study. Our population sample will answer the sixteen questions of the PROFFIT questionnaire, seven of which are related to the outcome and nine the determinants of FT. Data will be analyzed in the pediatric and adult populations and by group stratification. We are confident that this study will raise awareness among health-care professionals of the high risk of adverse health outcomes in patients who have both kidney disease and high levels of FT. Strategies to reduce FT should be implemented to improve the standard of care for people with kidney disease and lead to truly patient-centered care.
miRNAs as Interconnectors between Obesity and Cancer
Authors: González-Sánchez Grecia Denisse , Granados-López Angelica Judith , López-Hernández Yamilé , Robles Mayra Judith García , López Jesús Adrián
Published: April 2024

Obesity and cancer are a concern of global interest. It is proven that obesity may trigger the development or progression of some types of cancer; however, the connection by non-coding RNAs has not been totally explored. In the present review, we discuss miRNAs and lncRNAs dysregulation involved in obesity and some cancers, shedding light on how these conditions may exacerbate one another through the dysregulation of ncRNAs. lncRNAs have been reported as regulating microRNAs. An in silico investigation of lncRNA and miRNA interplay is presented. Our investigation revealed 44 upregulated and 49 downregulated lncRNAs in obesity and cancer, respectively. miR-375, miR-494-3p, miR-1908, and miR-196 were found interacting with 1, 4, 4 and 4 lncRNAs, respectively, which are involved in PPARγ cell signaling regulation. Additionally, miR-130 was found to be downregulated in obesity and reported as modulating 5 lncRNAs controlling PPARγ cell signaling. Similarly, miR-128-3p and miR-143 were found to be downregulated in obesity and cancer, interacting with 5 and 4 lncRNAs, respectively, associated with MAPK cell signaling modulation. The delicate balance between miRNA and lncRNA expression emerges as a critical determinant in the development of obesity-associated cancers, presenting these molecules as promising biomarkers. However, additional and deeper studies are needed to reach solid conclusions about obesity and cancer connection by ncRNAs.
Possible Involvement of Long Non-Coding RNAs GNAS-AS1 and MIR205HG in the Modulation of 5-Fluorouracil Chemosensitivity in Colon Cancer Cells through Increased Extracellular Release of Exosomes
Authors: Azwar Shamin , Ng Chin Tat , Zahari Sham Siti Yazmin , Seow Heng Fong , Chai Minhian , Ghazali Mohd Faizal , Jabar Mohd Faisal
Published: April 2024

A growing number of studies have suggested the involvement of long non-coding RNAs as the key players in not just the initiation and progression of the tumor microenvironment, but also in chemotherapy tolerance. In the present study, generated 5-FU-resistant SW480/DR cells were analyzed via cDNA microarray for its aberrant lncRNAs and mRNAs expression in comparison with the 5-FU-susceptible SW480/DS cells. Among the 126 lncRNAs described, lncRNAs GNAS-AS1, MIR205HG, and LOC102723721 have been identified to be significantly upregulated, while lncRNs lnc-RP11-597K23.2.1-2, LOC100507639, and CCDC144NL-AS1 have been found to be significantly downregulated. In the meantime, bioinformatic analysis through gene ontology studies of aberrantly expressed mRNAs revealed “regulated exocytosis”, among others, as the biological process most impacted in SW480/DR cells. To investigate, exosome purification was then carried out and its characterization were validated via transmission electron microscopy and nanoparticle tracking analysis. Interestingly, it was determined that the 5-FU-resistant SW480/DR cells secretes significantly higher concentration of extracellular vesicles, particularly, exosomes when compared to the 5-FU-susceptible SW480/DS cells. Based on the lncRNA-mRNA interaction network analysis generated, lncRNA GNAS-AS1 and MIR205HG have been identified to be potentially involved in the incidence of 5-FU resistance in SW480 colon cancer cells through promoting increased release of exosomes into the intercellular matrix. Our study hopes not only to provide insights on the list of involved candidate lncRNAs, but also to elucidate the role exosomes play in the initiation and development of 5-FU chemotherapy resistance in colon cancer cells.
The Last Mile in Polio Eradication: Program Challenges and Perseverance
Authors: Lopez Cavestany Rocio , Eisenhawer Martin , Diop Ousmane M. , Verma Harish , Quddus Arshad , Mach Ondrej
Published: April 2024

As the Global Polio Eradication Initiative (GPEI) strategizes towards the final steps of eradication, routine immunization schedules evolve, and high-quality vaccination campaigns and surveillance systems remain essential. New tools are consistently being developed, such as the novel oral poliovirus vaccine to combat outbreaks more sustainably, as well as non-infectiously manufactured vaccines such as virus-like particle vaccines to eliminate the risk of resurgence of polio on the eve of a polio-free world. As the GPEI inches towards eradication, re-strategizing in the face of evolving challenges and preparing for unknown risks in the post-certification era are critical.
Delivery of DNA-Based Therapeutics for Treatment of Chronic Diseases
Authors: Sussman Carleigh , Liberatore Rachel A. , Drozdz Marek M.
Published: April 2024

Gene therapy and its role in the medical field have evolved drastically in recent decades. Studies aim to define DNA-based medicine as well as encourage innovation and the further development of novel approaches. Gene therapy has been established as an alternative approach to treat a variety of diseases. Its range of mechanistic applicability is wide; gene therapy has the capacity to address the symptoms of disease, the body’s ability to fight disease, and in some cases has the ability to cure disease, making it a more attractive intervention than some traditional approaches to treatment (i.e., medicine and surgery). Such versatility also suggests gene therapy has the potential to address a greater number of indications than conventional treatments. Many DNA-based therapies have shown promise in clinical trials, and several have been approved for use in humans. Whereas current treatment regimens for chronic disease often require frequent dosing, DNA-based therapies can produce robust and durable expression of therapeutic genes with fewer treatments. This benefit encourages the application of DNA-based gene therapy to manage chronic diseases, an area where improving efficiency of current treatments is urgent. Here, we provide an overview of two DNA-based gene therapies as well as their delivery methods: adeno associated virus (AAV)-based gene therapy and plasmid DNA (pDNA)-based gene therapy. We will focus on how these therapies have already been utilized to improve treatment of chronic disease, as well as how current literature supports the expansion of these therapies to treat additional chronic indications in the future.
A CsPbI3/PCBM Phototransistor with Low Dark Current by Suppressing Ion Migration
Authors: Huang Chenbo , Yang Yichao , Li Yujie , Jiang Shijie , Yang Lurong , Li Ruixiao , She Xiaojian
Published: April 2024

Perovskite-based metal oxide phototransistors have emerged as promising photodetection devices owing to the superior optoelectronic properties of perovskite materials and the high carrier mobility of metal oxides. However, high dark current has been one major problem for this type of device. Here, we studied the dark current behaviors of phototransistors fabricated based on the Indium Gallium Zinc Oxide (IGZO) channel and different perovskite materials. We found that depositing organic–inorganic hybrid perovskites materials (MAPbI3/FAPbI3/FA0.2MA0.8PbI3) on top of IGZO transistor can increase dark current from ~10−6 mA to 1~10 mA. By contrast, we observed depositing an inorganic perovskite material, CsPbI3, incorporated with PCBM additive can suppress the dark current down to ~10−6 mA. Our study of ion migration reveals that ion migration is pronounced in organic–inorganic perovskite films but is suppressed in CsPbI3, particularly in CsPbI3 mixed with PCBM additive. This study shows that ion migration suppression by the exclusion of organic halide and the incorporation of PCBM additive can benefit low dark current in perovskite phototransistors.
Review of High-Power Continuous Wave Yb-Doped Fiber Lasers near 980 nm
Authors: Zhou Shangde , Cao Jianqiu , Chen Maoni , Wang Zefeng , Si Lei , Chen Jinbao
Published: April 2024

In this paper, the development of a high-power continuous wave (CW) fiber laser near 980 nm is reviewed. This review is focused primary on the power evolution resulting from the designation of Yb-doped fibers, which is important in the suppression of the amplified spontaneous emission (ASE) around 1030 nm. Current studies on the in-band ASE as the power limitation of the Yb-doped fiber lasers near 980 nm are also summarized in this review.
A 930 m/180 Gbps*User Underwater Coherent Optical Code-Division Multiple-Access Network Based on Hybrid 256-Differential Pulse Position Modulation and Weighted Modified Prime Code Sequence
Authors: Ismail Morsy Ahmed Morsy , Saleh Khalid
Published: April 2024

Currently, there are three types of optical communication networks based on the communication channel between the transmitter and receiver: the optical fiber channel, visible light channel, and optical wireless channel networks. The last type has several advantages for underwater communication, wireless sensors, and military communication networks. However, this type of optical network suffers from weather conditions in free-space communications and attenuation owing to the scattering and absorption mechanisms for underwater communication. In this study, we present a new transceiver architecture of a coherent optical code-division multiple-access (OCDMA) system based on a hybrid M-ary differential pulse position modulation scheme and a spreading code sequence called weighted modified prime code for underwater communication to minimize channel dispersion, increase the transmission rate per second, enhance the network bit error rate (BER) performance, and improve network security. Using an OCDMA system, we can simultaneously expand the network coverage area and increase the number of users sharing the network over the same channel bandwidth. The simulation results in this study proved that the proposed system can accommodate 1310 active users and a network throughput of 180 Gbps*user over a transmission distance of 930 m without any repeater at a 10−9 BER performance, compared to the 45 Gbps*user network throughput and 100 m transmission distance reported in the literature.
Dissipation-Induced Photon Blockade in the Anti-Jaynes–Cummings Model
Authors: Huang Biao , Li Cuicui , Fan Bixuan , Duan Zhenglu
Published: April 2024

Due to the fundamental differences between the quantum world and the classical world, some phenomena, such as entanglement and wave–particle duality, only exist in the quantum realm. These peculiar phenomena cannot be demonstrated by classical means: Quantum networks, quantum cryptography, and quantum precision measurements all require quantum sources. Photons are particularly well-suited as quantum sources owing to their minimal interaction with the environment, high flight speed, and ease of interaction with current typical quantum systems. Single-photon sources include pulsed excitation of quantum dots, spontaneous parametric down-conversion, and photon blockade. Herein, we propose that the anti-Jaynes–Cummings model can induce a pronounced photon antibunching effect when subjected to intense cavity dissipation. Similar to the photon blockade caused by strong photon–photon interaction, this antibunching effect is referred to as ’dissipation-induced blockade’. Our findings indicate that the minimum decay rate of a qubit, coupled with a high decay rate for photons, is conducive to achieving strong antibunching within the system. Notably, g(2)(0)<g(2)(τ), a characteristic of photon antibunching, is only valid under the optimal condition Δ=0. Conversely, g(2)(0)<1 is satisfied across all parameters, indicating that g(2)(0)<1 is not a prerequisite for antibunching in the anti-Jaynes–Cummings model. Moreover, under the optimal conditions of the antibunching effect, the average photon number attains its peak value. Consequently, the current anti-Jaynes–Cummings model is promising for developing single-photon sources characterized by excellent purity and average photon number.
QTLs and Genes for Salt Stress Tolerance: A Journey from Seed to Seed Continued
Authors: Tiwari Keshav , Tiwari Sushma , Kumar Nivesh , Sinha Shikha , Krishnamurthy Saraswathipura L. , Singh Renu , Kalia Sanjay , Singh Nagendra Kumar , Rai Vandna
Published: April 2024

Rice (Oryza sativa L.) is a crucial crop contributing to global food security; however, its production is susceptible to salinity, a significant abiotic stressor that negatively impacts plant germination, vigour, and yield, degrading crop production. Due to the presence of exchangeable sodium ions (Na+), the affected plants sustain two-way damage resulting in initial osmotic stress and subsequent ion toxicity in the plants, which alters the cell’s ionic homeostasis and physiological status. To adapt to salt stress, plants sense and transfer osmotic and ionic signals into their respective cells, which results in alterations of their cellular properties. No specific Na+ sensor or receptor has been identified in plants for salt stress other than the SOS pathway. Increasing productivity under salt-affected soils necessitates conventional breeding supplemented with biotechnological interventions. However, knowledge of the genetic basis of salinity stress tolerance in the breeding pool is somewhat limited because of the complicated architecture of salinity stress tolerance, which needs to be expanded to create salt-tolerant variants with better adaptability. A comprehensive study that emphasizes the QTLs, genes and governing mechanisms for salt stress tolerance is discussed in the present study for future research in crop improvement.
Stability Analysis and Identification of Superior Hybrids in Pearl Millet [Pennisetum glaucum (L.) R. Br.] Using the Multi Trait Stability Index
Authors: Khandelwal Vikas , Patel Rumit , Choudhary Khushwant B. , Pawar S. B. , Patel M. S. , Iyanar K. , Mungra K. D. , Kumar Sushil , Satyavathi C. Tara
Published: April 2024

Pearl millet stands as an important staple food and feed for arid and semi-arid regions of India and South Africa. It is also a quick supplier of important micronutrients like Fe and Zn via grain to combat micronutrient deficiencies among people in developing countries. India has notably spearheaded advancements in pearl millet production and productivity through the All India Coordinated Pearl Millet Improvement Project. There were 21 hybrids evaluated over arid and semi-arid ecologies of the western and southern regions of India. AMMI and GGE biplot models were adopted to recommend a specific hybrid for the particular locality. A joint analysis of variation indicated a significant genotype–environment interaction for most of the agronomical and grain micronutrient parameters. Pearson’s correlation values dissected the significant and positive correlation among agronomic traits and the negative correlation with grain micronutrient traits. GGE biplot analysis recommended the SHT 106 as a dual-purpose hybrid and SHT 115 as a biofortified hybrid for the grain’s Fe and Zn content. SHT 110 and SHT 108 were selected as stable and high grain yield-producing hybrids across all environments and specifically for E1, E2, and E4 as per the Which-Won-Where and What biplot. SHT 109 and SHT 103 hybrids were stable and high dry fodder yield-producing hybrids across all environments. In this study, the Multi-Trait Stability Index (MTSI) was employed to select the most stable and high-performing hybrids for all traits. It selected SHT 120, SHT 106, and SHT 104 for stability and great performance across all environments. These findings underscored the significance of tailored hybrid recommendations and the potential of pearl millet in addressing both food security and malnutrition challenges in various agro-ecological regions.
Complex Microlandscape as a Structural Unit of the Study of Spatiotemporal Development of an Ombrotrophic Suboceanic Bog
Authors: Ponomareva Tamara , Zubov Ivan , Shtang Anastasiya , Orlov Alexander , Selyanina Svetlana
Published: April 2024

Ombrotrophic suboceanic bogs are distinguished by a high diversity of complex microlandscapes within the bog massif. Each complex microlandscape is a separate intrabog ecosystem with a specific set of parameters and relationships. This study aims to assess the specifics of the characteristics and parameters of the complex microlandscapes as part of an ombrotrophic suboceanic Sphagnum bog and as stages of bog morphogenesis, and to establish the internal relationships and their relationship with external environmental factors. A comprehensive multidisciplinary approach was used to assess the functioning of the complex microlandscapes. It was found that the relationship with the air temperature is closer than with the bog water table dynamics. It was shown that the morphometric parameters of perennial dwarf shrubs can serve as indicators of the stages of development of bogs. The processes of the self-regulation of complex microlandscapes are weakened with the age of the complex microlandscape, as evidenced by an increase in the amplitude of temperature fluctuations and the level of bog waters, as well as the key physicochemical parameters of the peat deposit. This leads to a gradual, slow reorientation of the physicochemical processes occurring in the deposit, from the deposition of organic matter to the decomposition of peat biomass.
A Compact Handheld Sensor Package with Sensor Fusion for Comprehensive and Robust 3D Mapping
Authors: Wei Peng , Fu Kaiming , Villacres Juan , Ke Thomas , Krachenfels Kay , Stofer Curtis Ryan , Bayati Nima , Gao Qikai , Zhang Bill , Vanacker Eric , Kong Zhaodan
Published: April 2024

This paper introduces an innovative approach to 3D environmental mapping through the integration of a compact, handheld sensor package with a two-stage sensor fusion pipeline. The sensor package, incorporating LiDAR, IMU, RGB, and thermal cameras, enables comprehensive and robust 3D mapping of various environments. By leveraging Simultaneous Localization and Mapping (SLAM) and thermal imaging, our solution offers good performance in conditions where global positioning is unavailable and in visually degraded environments. The sensor package runs a real-time LiDAR-Inertial SLAM algorithm, generating a dense point cloud map that accurately reconstructs the geometric features of the environment. Following the acquisition of that point cloud, we post-process these data by fusing them with images from the RGB and thermal cameras and produce a detailed, color-enriched 3D map that is useful and adaptable to different mission requirements. We demonstrated our system in a variety of scenarios, from indoor to outdoor conditions, and the results showcased the effectiveness and applicability of our sensor package and fusion pipeline. This system can be applied in a wide range of applications, ranging from autonomous navigation to smart agriculture, and has the potential to make a substantial benefit across diverse fields.
MEMS Resonant Beam with Outstanding Uniformity of Sensitivity and Temperature Distribution for Accurate Gas Sensing and On-Chip TGA
Authors: Lu Zheng , Jia Hao , Wang Ding , Yu Haitao
Published: April 2024

Micromechanical resonators have aroused growing interest as biological and chemical sensors, and microcantilever beams are the main research focus. Recently, a resonant microcantilever with an integrated heater has been applied in on-chip thermogravimetric analysis (TGA). However, there is a strong relationship between the mass sensitivity of a resonant microcantilever and the location of adsorbed masses. Different sampling positions will cause sensitivity differences, which will result in an inaccurate calculation of mass change. Herein, an integrated H-shaped resonant beam with uniform mass sensitivity and temperature distribution is proposed and developed to improve the accuracy of bio/chemical sensing and TGA applications. Experiments verified that the presented resonant beam possesses much better uniformity of sensitivity and temperature distribution compared with resonant microcantilevers. Gas-sensing and TGA experiments utilizing the integrated resonant beam were also carried out and exhibited good measurement accuracy.
Full-Section Deformation Monitoring of High-Altitude Fault Tunnels Based on Three-Dimensional Laser Scanning Technology
Authors: Tan Dongmei , Tao Yu , Ji Baifeng , Gan Qinlin , Guo Tai
Published: April 2024

In traditional tunnel monitoring, the characteristic points of an object within a tunnel are measured to obtain information about the object. Considering the limitations of the traditional method in measuring the complex surface structure of tunnels, such as limited monitoring points, a long measurement period, and low precision, this study introduces an approach that uses three-dimensional (3D) laser scanning for monitoring tunnel cross-section deformation. Using this approach, the soft surrounding rock of a high-altitude ultralong tunnel was taken as the monitoring object. The test tunnel was first scanned using a 3D laser scanner, and the collected data were processed. The internal structural data of the tunnel were subsequently compared with its actual contour lines and the data of its primary branch and secondary lining on different dates. The results indicate that the arch roof of the tunnel tended to be stable within a certain time range when the positions of the primary branch and secondary lining were at different measuring points with different pile numbers. The deformation of the pile number on the left and right sides did not generally exceed 0.02 m, except at a few measuring points. A comparison between the actual cross section of the initial branch and that of the designed section showed that the actual elevation of the arch of the initial branch of the tunnel was greater than its designed elevation by no more than 0.3 m. Hence, through this study, a convenient and practical method is presented for monitoring deformation in complex curved tunnel structures.
Improving Aerosol Characterization Using an Optical Particle Counter Coupled with a Quartz Crystal Microbalance with an Integrated Microheater
Authors: Zampetti Emiliano , Mancuso Maria Aurora , Capocecera Alessandro , Papa Paolo , Macagnano Antonella
Published: April 2024

Aerosols, as well as suspended particulate matter, impact atmospheric pollution, the climate, and human health, directly or indirectly. Particle size, chemical composition, and other aerosol characteristics are determinant factors for atmospheric pollution dynamics and more. In the last decade, low-cost devices have been widely used in instrumentation to measure aerosols. However, they present some issues, such as the problem of discriminating whether the aerosol is composed of liquid particles or solid. This issue could lead to errors in the estimation of mass concentration in monitoring environments where there is fog. In this study, we investigate the use of an optical particle counter (OPC) coupled to a quartz crystal microbalance with an integrated microheater (H-QCM) to enhance measurement performances. The H-QCM was used not only to measure the collected mass on its surface but also, by using the integrated microheater, it was able to heat the collected mass by performing heating cycles. In particular, we tested the developed system with aerosolized saline solutions of sodium chloride (NaCl), with three decreasing concentrations of salt and three electronic cigarette solutions (e-liquid), with different concentrations of propylene glycol and glycerin mixtures. The results showed that the OPC coherently counted the salt dilution effects, and the H-QCM output confirmed the presence of liquid and solid particles in the aerosols. In the case of e-liquid aerosols, the OPC counted the particles, and the HQCM output highlighted that in the aerosol, there were no solid particles but a liquid phase only. These findings contribute to the refinement of aerosol measurement methodologies by low-cost sensors, fostering a more comprehensive understanding.
A Synchrosqueezed Transform Method Based on Fast Kurtogram and Demodulation and Piecewise Aggregate Approximation for Bearing Fault Diagnosis
Authors: Chen Yanlu , Hu Lei , Hu Niaoqing , Zeng Jiyu
Published: April 2024

Synchrosqueezed transform (SST) is a time–frequency analysis method that can improve energy aggregation and reconstruct signals, which has been applied in the fields of medical treatment, fault diagnosis, and seismic wave processing. However, when dealing with time-varying signals, SST suffers from poor time–frequency resolution and is unable to deal with long signals. In order to accurately extract the characteristic frequency of variable speed rolling bearing faults, this paper proposes a synchrosqueezed transform method based on fast kurtogram and demodulation and piecewise aggregate approximation (PAA). The method firstly filters and demodulates the original signal using fast kurtogram and Hilbert transform to reduce the influence of background noise and improve the time–frequency resolution. Then, it compresses the signal by using piecewise aggregate approximation, so that the SST can deal with long signals and, thus, extract the fault characteristic frequency. The experimental data verification results indicate that the method can effectively identify the fault characteristic frequency of variable-speed rolling bearings.