bluuebunny/crossref_metadata_2025 huggingface dataset:
The Influence of Co-Fermentation of Agri-Food Waste with Primary Sludge on Biogas Production and Composition of the Liquid Fraction of Digestate
Authors: Wilinska-Lisowska Anna , Ossowska Monika , Czerwionka Krzysztof
Published: March 2021

Energy self-sufficiency is a current trend in wastewater treatment plants. This effect can be achieved by increasing the production of electricity from biogas and by reducing energy consumption for technological processes. One idea, in line with the circular economy concept, is the use of waste rich in organic matter as co-substrates for the fermentation process. The aim of this study was to determine the effect of waste co-fermentation on biogas production and nitrogen concentration in the reject water. A co-fermentation process with flotate or flotate and vegetables increased biogas production compared to primary sludge by 162 and 180%, respectively. During the tests, there was no inhibition of the fermentation process. Hydrolysis of organic compounds contained in flotate and vegetables resulted in a significant increase in ammonium nitrogen (by 80–100%) and dissolved organic nitrogen concentration (by 170–180%). The biogas and methane production rate as well as the ammonium and total nitrogen release rate were calculated. An energy balance was made, which took into account the variable amount of electric energy production depending on the efficiency of the cogeneration systems and energy consumption for supplying oxygen necessary to remove nitrogen contained in the reject water. A positive energy balance was obtained for all analyses.
Risk Assessment and Management Workflow—An Example of the Southwest Regional Partnership
Authors: Lee Si-Yong , Hnottavange-Telleen Ken , Jia Wei , Xiao Ting , Viswanathan Hari , Chu Shaoping , Dai Zhenxue , Pan Feng , McPherson Brian , Balch Robert
Published: March 2021

This paper summarizes the risk assessment and management workflow developed and applied to the Southwest Regional Partnership on Carbon Sequestration (SWP) Phase III Demonstration Project. The risk assessment and management workflow consists of six primary tasks, including management planning, identification, qualitative analysis, quantitative analysis, response planning, and monitoring. Within the workflow, the SWP assembled and iteratively updated a risk registry that identifies risks for all major activities of the project. Risk elements were ranked with respect to the potential impact to the project and the likelihood of occurrence. Both qualitative and quantitative risk analyses were performed. To graphically depict the interactions among risk elements and help building risk scenarios, process influence diagrams were used to represent the interactions. The SWP employed quantitative methods of risk analysis including Response Surface Method (RSM), Polynomial Chaos Expansion (PCE), and the National Risk Assessment Partnership (NRAP) toolset. The SWP also developed risk response planning and performed risk control and monitoring to prevent the risks from affecting the project and ensure the effectiveness of risk management. As part of risk control and monitoring, existing and new risks have been tracked and the response plan was subsequently evaluated. Findings and lessons learned from the SWP’s risk assessment and management efforts will provide valuable information for other commercial geological CO2 storage projects.
Defensible Spaces
Authors: Moanis Yasmin , Hussein Fatmaelzahraa
Published: March 2021

Defensible space is the concept of creating spaces that include a series of architectural guidelines that are used when designing a new urban residential area to promote both the territorial claim of the residential groups to their surroundings and their ability to conduct natural surveillance of their spaces. Constructing safe environments with safe vocabularies for enhancing well-being is crucial for achieving quality of life in both peace and wartime. Warzone countries that suffer from many changes due to the effect of war (or fourth-generation wars) should be prepared to face those changes with safe spaces as well.
Soil Microbiome Composition along the Natural Norway Spruce Forest Life Cycle
Authors: Choma Michal , Šamonil Pavel , Kaštovská Eva , Bárta Jiří , Tahovská Karolina , Valtera Martin , Šantrůčková Hana
Published: March 2021

Stand-replacing disturbances are a key element of the Norway spruce (Picea abies) forest life cycle. While the effect of a natural disturbance regime on forest physiognomy, spatial structure and pedocomplexity was well described in the literature, its impact on the microbiome, a crucial soil component that mediates nutrient cycling and stand productivity, remains largely unknown. For this purpose, we conducted research on a chronosequence of sites representing the post-disturbance development of a primeval Norway spruce forest in the Calimani Mts., Romania. The sites were selected along a gradient of duration from 16 to 160 years that ranges from ecosystem regeneration phases of recently disturbed open gaps to old-growth forest stands. Based on DNA amplicon sequencing, we followed bacterial and fungal community composition separately in organic, upper mineral and spodic horizons of present Podzol soils. We observed that the canopy opening and subsequent expansion of the grass-dominated understorey increased soil N availability and soil pH, which was reflected in enlarged bacterial abundance and diversity, namely due to the contribution of copiotrophic bacteria that prefer nutrient-richer conditions. The fungal community composition was affected by the disturbance as well but, contrary to our expectations, with no obvious effect on the relative abundance of ectomycorrhizal fungi. Once the mature stand was re-established, the N availability was reduced, the pH gradually decreased and the original old-growth forest microbial community dominated by acidotolerant oligotrophs recovered. The effect of the disturbance and forest regeneration was most evident in organic horizons, while the manifestation of these events was weaker and delayed in deeper soil horizons.
Precommercial Thinning Increases Spruce Yields in Boreal Mixedwoods in Alberta, Canada
Authors: Bjelanovic Ivan , Comeau Phil , Meredith Sharon , Roth Brian
Published: March 2021

A few studies in young mixedwood stands demonstrate that precommercial thinning of aspen at early ages can improve the growth of spruce and increase stand resilience to drought. However, information on tree and stand responses to thinning in older mixedwood stands is lacking. To address this need, a study was initiated in 2008 in Alberta, Canada in 14 boreal mixedwood stands (seven each at ages 17 and 22). This study investigated growth responses following thinning of aspen to five densities (0, 1000, 2500, 5000 stems ha−1 and unthinned (control)). Measurements were collected in the year of establishment, and three and eight years later. Mortality of aspen in the unthinned plots was greater than in the thinned plots which were not significantly different amongst each other. Eight years following treatment, aspen diameter was positively influenced by thinning, while there was no effect on aspen height. The density of aspen had no significant effect on the survival of planted spruce. Spruce height and diameter growth increased with both aspen thinning intensity and time since treatment. Differentiation among treatments in spruce diameter growth was evident three years from treatment, while differentiation in height was not significant until eight years following treatment. Yield projections using two growth models (Mixedwood Growth Model (MGM) and Growth and Yield Projection System (GYPSY)) were initialized using data from the year eight re-measurements. Results indicate that heavy precommercial aspen thinning (to ~1000 aspen crop trees ha−1) can result in an increase in conifer merchantable volume without reducing aspen volume at the time of harvest. However, light to moderate thinning (to ~2500 aspen stems ha−1 or higher), is unlikely to result in gains in either deciduous or conifer merchantable harvest volume over those of unthinned stands.
Genome-Wide Analysis of Poplar SQUAMOSA-Promoter-Binding Protein (SBP) Family under Salt Stress
Authors: Guo Qing , Li Li , Zhao Kai , Yao Wenjing , Cheng Zihan , Zhou Boru , Jiang Tingbo
Published: March 2021

SQUAMOSA promoter binding protein (SBP) is a kind of plant-specific transcription factor, which plays a crucial role in stress responses and plant growth and development by activating and inhibiting the transcription of multiple target genes. In this study, a total of 30 SBP genes were identified from Populus trichocarpa genome and randomly distributed on 16 chromosomes in poplar. According to phylogenetic analysis, the PtSBPs can be divided into six categories, and 14 out of the genes belong to VI. Furthermore, the SBP genes in VI were proved to have a targeting relationship with miR156. The homeopathic element analysis showed that the promoters of poplar SBP genes mainly contain the elements involved in growth and development, abiotic stress and hormone response. In addition, there existed 10 gene segment duplication events in the SBP gene duplication analysis. Furthermore, there were four poplar and Arabidopsis orthologous gene pairs among the poplar SBP members. What is more, poplar SBP gene family has diverse gene expression pattern under salt stress. As many as nine SBP members were responding to high salt stress and six members possibly participated in growth development and abiotic stress. Yeast two-hybrid experiments indicated that PtSBPs can form heterodimers to interact in the transcriptional regulatory networks. The genome-wide analysis of poplar SBP family will contribute to function characterization of SBP genes in woody plants.
Economic Impacts of Forest Storms—Taking Stock of After-Vaia Situation of Local Roundwood Markets in Northeastern Italy
Authors: Udali Alberto , Andrighetto Nicola , Grigolato Stefano , Gatto Paola
Published: March 2021

Large timber availability after storms can lead to a drop in timber prices that can impact local markets. The Vaia storm, which occurred in Northeastern Italy at the end of October 2018, felled large volumes of timber, particularly spruce. To estimate the loss in volume and value connected to Vaia, data of roundwood sales from four local markets (Province of Trento, Province of Bolzano, Veneto Region and Friuli Venezia Giulia Region) were collected before and after Vaia, as well as reports on the status of salvage operations. The results confirm that Vaia had a strong impact on Northeastern Italy. A large area of the forest was hit and massive volumes of wood were affected. The analysis of prices showed a negative trend in the post-Vaia period due to an increase in volume per sale, especially for stumpage sales. It also highlighted the difficulties of storing the salvaged wood and the consequent fast saturation of the market. Although it was not possible to assess long-term effects on the four local markets, the presence and use of local e-commerce platforms proved useful in making roundwood sales more organized and efficient.
Spatial Heterogeneity of Plant Diversity within and between Neighborhoods and Its Implications for a Plant Diversity Survey in Urban Areas
Authors: Su Yuebo , Gong Cheng , Cui Bowen , Guo Peipei , Ouyang Zhiyun , Wang Xiaoke
Published: March 2021

The spatial heterogeneity of plant diversity at the neighborhood scale has less been understood, although it is very important for the planning and management of neighborhood landscape. In this case study of Beijing, we conducted intensive investigations of the plant diversity in different neighborhoods along a rural–urban gradient. The results showed that the mean numbers of plant species per neighborhood were 30.5 for trees, 18.8 for shrubs, and 31.9 for herbs, respectively. There were significant logarithmic relationships between the numbers of species and patch area, indicating that larger patches within neighborhoods could harbor more plant species. Hierarchical linear modeling showed that the variations in plant diversity within neighborhoods were higher than those between neighborhoods. The number of species increased logistically with both the number of patches within neighborhoods and the number of neighborhoods, suggesting that it is important to sample a sufficient number of patches within neighborhoods, as well as a sufficient number of neighborhoods in order to sample 90% of the plant species during the investigation of plant diversity in urban neighborhoods. So the hierarchical design of sampling should be recommended for investigating plant diversity in urban areas.
Research on the Impacts of Generalized Preceding Vehicle Information on Traffic Flow in V2X Environment
Authors: Wang Xiaoyuan , Han Junyan , Bai Chenglin , Shi Huili , Zhang Jinglei , Wang Gang
Published: March 2021

With the application of vehicles to everything (V2X) technologies, drivers can obtain massive traffic information and adjust their car-following behavior according to the information. The macro-characteristics of traffic flow are essentially the overall expression of the micro-behavior of drivers. There are some shortcomings in the previous researches on traffic flow in the V2X environment, which result in difficulties to employ the related models or methods in exploring the characteristics of traffic flow affected by the information of generalized preceding vehicles (GPV). Aiming at this, a simulation framework based on the car-following model and the cellular automata (CA) is proposed in this work, then the traffic flow affected by the information of GPV is simulated and analyzed utilizing this framework. The research results suggest that the traffic flow, which is affected by the information of GPV in the V2X environment, would operate with a higher value of velocity, volume as well as jamming density and can maintain the free flow state with a much higher density of vehicles. The simulation framework constructed in this work can provide a reference for further research on the characteristics of traffic flow affected by various information in the V2X environment.
DEEPGENTM—A Novel Variant Calling Assay for Low Frequency Variants
Authors: Hermann Bernd Timo , Pfeil Sebastian , Groenke Nicole , Schaible Samuel , Kunze Robert , Ris Frédéric , Hagen Monika Elisabeth , Bhakdi Johannes
Published: March 2021

Detection of genetic variants in clinically relevant genomic hot-spot regions has become a promising application of next-generation sequencing technology in precision oncology. Effective personalized diagnostics requires the detection of variants with often very low frequencies. This can be achieved by targeted, short-read sequencing that provides high sequencing depths. However, rare genetic variants can contain crucial information for early cancer detection and subsequent treatment success, an inevitable level of background noise usually limits the accuracy of low frequency variant calling assays. To address this challenge, we developed DEEPGENTM, a variant calling assay intended for the detection of low frequency variants within liquid biopsy samples. We processed reference samples with validated mutations of known frequencies (0%–0.5%) to determine DEEPGENTM’s performance and minimal input requirements. Our findings confirm DEEPGENTM’s effectiveness in discriminating between signal and noise down to 0.09% variant allele frequency and an LOD(90) at 0.18%. A superior sensitivity was also confirmed by orthogonal comparison to a commercially available liquid biopsy-based assay for cancer detection.
Dynamic Expression of Imprinted Genes in the Developing and Postnatal Pituitary Gland
Authors: Scagliotti Valeria , Esse Ruben , Willis Thea L. , Howard Mark , Carrus Isabella , Lodge Emily , Andoniadou Cynthia L. , Charalambous Marika
Published: March 2021

In mammals, imprinted genes regulate many critical endocrine processes such as growth, the onset of puberty and maternal reproductive behaviour. Human imprinting disorders (IDs) are caused by genetic and epigenetic mechanisms that alter the expression dosage of imprinted genes. Due to improvements in diagnosis, increasing numbers of patients with IDs are now identified and monitored across their lifetimes. Seminal work has revealed that IDs have a strong endocrine component, yet the contribution of imprinted gene products in the development and function of the hypothalamo-pituitary axis are not well defined. Postnatal endocrine processes are dependent upon the production of hormones from the pituitary gland. While the actions of a few imprinted genes in pituitary development and function have been described, to date there has been no attempt to link the expression of these genes as a class to the formation and function of this essential organ. This is important because IDs show considerable overlap, and imprinted genes are known to define a transcriptional network related to organ growth. This knowledge deficit is partly due to technical difficulties in obtaining useful transcriptomic data from the pituitary gland, namely, its small size during development and cellular complexity in maturity. Here we utilise high-sensitivity RNA sequencing at the embryonic stages, and single-cell RNA sequencing data to describe the imprinted transcriptome of the pituitary gland. In concert, we provide a comprehensive literature review of the current knowledge of the role of imprinted genes in pituitary hormonal pathways and how these relate to IDs. We present new data that implicate imprinted gene networks in the development of the gland and in the stem cell compartment. Furthermore, we suggest novel roles for individual imprinted genes in the aetiology of IDs. Finally, we describe the dynamic regulation of imprinted genes in the pituitary gland of the pregnant mother, with implications for the regulation of maternal metabolic adaptations to pregnancy.
The Formation of Biofilm and Bacteriology in Otitis Media with Effusion in Children: A Prospective Cross-Sectional Study
Authors: Niedzielski Artur , Chmielik Lechosław Paweł , Stankiewicz Tomasz
Published: March 2021

Background: Otitis media with effusion (OME) can cause serious complications such as hearing impairment or development delays. The aim of the study was to assess the microbiological profile of organisms responsible for OME and to determine if a biofilm formation can be observed. Methods: Ninety-nine samples from 76 patients aged from 6 months to 12 years were collected for microbiological and molecular studies. Results: In microbiological studies, pathogenic bacteria Haemophilus influenzae (38.89%), Streptococcus pneumoniae (33.33%), and Staphylococcus aureus MSSA (27.78%), as well as opportunistic bacteria Staphylococcus spp. (74.14%), Diphtheroids (20.69%), Streptococcus viridans (3.45%), and Neisseria spp. (1.72%) were found. The average degree of hearing loss in the group of children with positive bacterial culture was 35.9 dB, while in the group with negative bacterial culture it was 25.9 dB (p = 0.0008). The type of cultured bacteria had a significant impact on the degree of hearing impairment in children (p = 0.0192). In total, 37.5% of Staphylococcus spp. strains were able to form biofilm. Conclusions: Staphylococcus spp. in OME may form biofilms, which can explain the chronic character of the disease. Pathogenic and opportunistic bacteria may be involved in the etiopathogenesis of OME. The degree of hearing loss was significantly higher in patients from which the positive bacterial cultures were obtained.
“There Is a Lot of Practice in Not Thinking about That”: Structural, Interpersonal, and Individual-Level Barriers to HIV/STI Prevention among Reservation Based American Indians
Authors: Armenta Richard F , Kellogg Daniel , Montoya Jessica L , Romero Rick , Armao Shandiin , Calac Daniel , Gaines Tommi L
Published: March 2021

American Indians (AI) face significant disparities in HIV/STI morbidity and mortality, and historical, structural, interpersonal, and individual level barriers stymie prevention efforts. The objective of this paper is to examine barriers to HIV/STI prevention among reservation-based AI. We conducted face-to-face qualitative interviews with 17 reservation-based AI community leaders and community members in Southern California on HIV/STI knowledge and attitudes and barriers to prevention. The disruption of traditional coping mechanisms and healing processes were compromised by historical trauma, and this allowed stigmas to exist where they did not exist before. This impacted access to healthcare services and trust in medicine, and is linked to individuals adopting negative coping behaviors that confer risk for HIV/STI transmission (e.g., substance use and sexual behaviors). Most of the participants reported that HIV/STIs were not discussed in their reservation-based communities, and many participants had a misperception of transmission risk. Stigma was also linked to a lack of knowledge and awareness of HIV/STI’s. Limited available services, remoteness of communities, perceived lack of privacy, and low cultural competency among providers further hindered the access and use of HIV/STI prevention services. These findings highlight the need to address the historical, structural, and interpersonal factors impacting individual-level behaviors that can increase HIV/STI transmission among reservation-based AIs. Prevention work should build on community strengths to increase HIV/STI knowledge, reduce stigma, and increase access to preventative care while using culturally grounded methodologies.
Psychological Health Status of Psychiatric Patients Living in Treatment Communities before and during the COVID-19 Lockdown: A Brief Report
Authors: Cordellieri Pierluigi , Barchielli Benedetta , Masci Valeria , Viani Francesca , de Pinto Ivan , Priori Andrea , Torriccelli Felice Damiano , Cosmo Chiara , Ferracuti Stefano , Giannini Anna Maria , Burrai Jessica
Published: March 2021

Many studies investigated the psychological impact of lockdown measures on the general population, while few studies focused on the psychiatric population. This study aimed to investigate the role of therapeutic communities in the management and containment of symptoms of patients with psychosis living in psychiatric residential facilities. Data were collected at two different points: November 2019 (Coronavirus disease 19 had not yet spread) and April 2020 (during the lockdown in Italy). Twenty-two study participants were recruited from three residential accredited psychiatric facilities. During lockdown, the patients showed a small increase in symptomatology in terms of emotional isolation. In addition, it was been observed significant differences in certain functional areas of the behavior, measured as lower inclination towards violent behaviors during lockdown, and higher scores in substance abuse and medical impairment. The lockdown condition could represent a form of containment; daily routines, along with adequate social support, are important aspects of the stability and the level of behavioral functioning of psychiatric patients. Social support and continuity of care offered by psychiatric communities can be an effective safeguard against the psychological impact of the COVID-19 epidemic.
Exercise Intensity in Patients with Cardiovascular Diseases: Systematic Review with Meta-Analysis
Authors: Gonçalves Catarina , Raimundo Armando , Abreu Ana , Bravo Jorge
Published: March 2021

Exercise-induced improvements in the VO2peak of cardiac rehabilitation participants are well documented. However, optimal exercise intensity remains doubtful. This study aimed to identify the optimal exercise intensity and program length to improve VO2peak in patients with cardiovascular diseases (CVDs) following cardiac rehabilitation. Randomized controlled trials (RCTs) included a control group and at least one exercise group. RCTs assessed cardiorespiratory fitness (CRF) changes resulting from exercise interventions and reported exercise intensity, risk ratio, and confidence intervals (CIs). The primary outcome was CRF (VO2peak or VO2 at anaerobic threshold). Two hundred and twenty-one studies were found from the initial search (CENTRAL, MEDLINE, CINAHL and SPORTDiscus). Following inclusion criteria, 16 RCTs were considered. Meta-regression analyses revealed that VO2peak significantly increased in all intensity categories. Moderate-intensity interventions were associated with a moderate increase in relative VO2peak (SMD = 0.71 mL-kg−1-min−1; 95% CI = [0.27–1.15]; p = 0.001) with moderate heterogeneity (I2 = 45%). Moderate-to-vigorous-intensity and vigorous-intensity interventions were associated with a large increase in relative VO2peak (SMD = 1.84 mL-kg−1-min−1; 95% CI = [1.18–2.50], p < 0.001 and SMD = 1.80 mL-kg−1-min−1; 95% CI = [0.82–2.78] p = 0.001, respectively), and were also highly heterogeneous with I2 values of 91% and 95% (p < 0.001), respectively. Moderate-to-vigorous and vigorous-intensity interventions, conducted for 6–12 weeks, were more effective at improving CVD patients’ CRF.
Normative Data of Objectively Measured Physical Activity and Sedentary Time in Community—Dwelling Older Japanese
Authors: Yatsugi Harukaze , Chen Tao , Chen Si , Narazaki Kenji , Nagayoshi Sho , Kumagai Shuzo , Kishimoto Hiro
Published: March 2021

Background: The amounts of moderate-to-vigorous-intensity physical activity (MVPA), light-intensity physical activity (LPA), and sedentary time (ST) by sex, age, and body mass index (BMI) in older Japanese adults have not been known. We conducted this study to determine the actual physical activity (PA) and ST in this population. Subjects and Methods: A total of 3998 community-dwelling Japanese adults aged ≥65 years were investigated. Their levels of PA and ST and number of steps taken daily were assessed for seven consecutive days by a tri-axial accelerometer. Normative values of daily PA and ST were analyzed by age and BMI groups in the men and the women and are presented as mean, median, or decile. Results: The subjects generally adhered to the PA guideline, i.e., ≥10 metabolic equivalents (METs)·hour MVPA per week. Older age was associated with lower adherence to the PA guideline. Conclusions: Normative values (mean, median, or decile) were yielded for MVPA, LPA, and ST based on accelerometer readings in a large sample of older community-dwelling Japanese adults. One-half of the subjects’ waking time was spent being sedentary, and >70% of the subjects met the current PA guideline by engaging in MVPA.
Effect of a Shock Micro-Cycle on Biochemical Markers in University Soccer Players
Authors: García-Cardona Diana , Landázuri Patricia , Sánchez-Muñoz Oscar
Published: March 2021

This study aimed to examine various biochemical biomarkers changes during a shock micro-cycle in soccer players from a university team. The study had 22 players (age: 22 ± 3 years; body mass: 68.6 ± 7.1 kg; height: 1.73 ± 0.07 m). The study measured total cholesterol (TC), triglycerides (TG), cholesterol linked to high-density lipoproteins (HDL), low-density lipoproteins (LDL), very low density lipoproteins (VLDL), arterial index (AI), creatine kinase (CK), glutamate-oxalacetate-transaminase (GOT), glutamate-pyruvate-transaminase (GPT), creatinine (Cr), catalase (CAT), superoxide dismutase (SOD), cytokines IL6 and TNFα, total antioxidant capacity (Cap antiox tot), hemolysis percentage and glomerular filtration rate (GFR); measurements were conducted during a shock micro-cycle. The lipid profile variables had no statistical significance when compared on day 1 with day 14. Except for TNFα, the other biomarkers compared with day one had progressive increments until day seven, with a subsequent reduction on day 14; however, none of the biomarkers returned to baseline values despite this decrease. The data shown herein suggest the need to research these biomarkers in distinct types of mesocycles, exercise, intensity, load, and duration to diminish fatigue and improve athlete performance.
Factors Predicting Uptake of Sexually Transmitted Infections Testing among Men Who Have Sex with Men Who Are “Pre-Exposure Prophylaxis Tourists”—An Observational Prospective Cohort Study
Authors: Wang Zixin , Fang Yuan , Yaemim Natthakhet , Jonas Kai J. , Chidgey Andrew , Ip Mary , Cheng Tommy , Lau Joseph T. F.
Published: March 2021

The term “Pre-exposure prophylaxis (PrEP) tourists” refers to individuals who obtain PrEP in other countries and use it in their home countries. A prospective observational cohort study was conducted among a group of men who have sex with men (MSM) who obtained PrEP in private clinics in Thailand and used it in Hong Kong. Participants completed two web-based self-administered surveys when obtaining PrEP in Thailand and three months afterwards. Out of 110 participants at baseline, 67 completed the follow-up. The prevalence of sexually transmitted infection (STI) testing was 47.8% during the follow-up period. Eleven participants received an STI diagnosis, and seven of them were incident infections in the past three months. Participants who perceived a higher chance for STI infection (adjusted odds ratios (AOR): 1.90, 95% CI: 1.00, 3.75) and reported higher intention to take up STI testing at baseline (AOR: 1.62, 95% CI: 1.05, 2.50) were more likely to receive STI testing during the follow-up period. Baseline perceptions that service providers would think they were having risky behaviors because of PrEP use was negatively associated with the dependent variable (AOR: 0.51, 95% CI: 0.31, 0.86). Service planning and health promotion related to STI testing is needed for MSM “PrEP tourists”.
Association between Psychopathological Dimensions and Sexual Functioning/Sexual Arousal in Young Adults
Authors: Soler Franklin , Granados Reina , Arcos-Romero Ana I. , Calvillo Cristóbal , Álvarez-Muelas Ana , Sánchez-Fuentes María del Mar , Moyano Nieves , Sierra Juan Carlos
Published: March 2021

Psychological-psychiatric factors have a different effect on sexual functioning in men and women. This research aimed to examine the association between psychopathological dimensions and dimensions of sexual functioning in Spanish young adults in two studies. Study 1 examined sexual functioning and psychopathological dimensions in 700 women and 516 men. Study 2 conducted an experimental laboratory task to evaluate subjective sexual arousal and genital sensations when watching visual sexual stimuli in a subsample of participants from Study 1 (143 women and 123 men). As a result, the first study showed that depression and anxiety-related symptoms had a negative effect, both in men and women, and having a partner had a positive influence on the dimensions of sexual functioning. The second study showed that anxiety symptoms were positively associated with subjective sexual arousal in both men and women, and anxiety was associated with the assessment of genital sensations in men. The differences between the results of anxiety may be explained because sexual arousal was evaluated in general terms in Study 1, whereas it was evaluated as a state in Study 2. These findings confirm that the presence of psychopathological symptoms contributes to sexual functioning, as well as the necessity of strengthening mental illness prevention programs that include sexual health components.
Increased Risk of Traumatic Injuries among Parents of Children with Attention Deficit/Hyperactivity Disorder: A Nationwide Population-Based Study
Authors: Li Dian-Jeng , Chen Yi-Lung , Chen Ying-Yeh , Hsiao Ray C. , Lu Wei-Hsin , Yen Cheng-Fang
Published: March 2021

Children with attention deficit/hyperactivity disorder (ADHD) are vulnerable to traumatic injuries. Parents of children with ADHD experience undesirable impacts more frequently than parents of children without ADHD. The aim of this study was to evaluate whether traumatic injuries are more prevalent in parents of children with ADHD than in parents of children without ADHD. We compared the prevalence of traumatic injuries between parents of children with and without ADHD by using data from the Taiwan Maternal and Child Health Database from 2004 to 2017. The Cox proportional-hazards regression model was used to examine differences in burn injury, fracture, and traumatic brain injury between parents of children with and without ADHD after adjustment for age, urbanicity, and income level. In total, 81,401 fathers and 87,549 mothers who had at least one offspring with ADHD and 1,646,100 fathers and 1,730,941 mothers with no offspring with ADHD were included in the analysis. The results indicated that both fathers and mothers of children with ADHD had higher risks of burn injury, fracture, and traumatic brain injury than fathers and mothers of children without ADHD. Mothers of children with ADHD had higher risks for all kinds of traumatic events than fathers of children with ADHD.
Exploring Food Literacy Domains in an Adult Samoan Population
Authors: Kammholz Grace , Craven Dana , Boodoosingh Ramona , Akeli Amaama Safua , Abraham Jyothi , Burkhart Sarah
Published: March 2021

Samoan food systems have undergone a dramatic nutrition transition, with dietary patterns changing concurrently with increased rates of obesity and non-communicable disease. Whilst policy action and environmental interventions play an important role in improving access to and consumption of healthy food, the success of these relies on a greater understanding of individuals’ food knowledge and behaviours. This study aimed to explore these behaviours using the construct of food literacy in an adult Samoan population. A cross-sectional interviewer-administered questionnaire of a convenience sample of 150 adult Samoans (≥20 years) assessed the four domains of food literacy: plan/manage, select, prepare, and eat. Participants generally plan to include healthy food (87%) and budget money for food (87%). The majority know where to find nutrition labels (68%), of which 43% always use them to inform their food choices. Participants were mostly confident with cooking skills, although food storage practices require further investigation. Over 90% agreed or strongly agreed that food impacts health, although understanding of the Pacific Guidelines for Healthy Living was lacking. Understanding the ability of Samoans to plan/manage, select, prepare, and eat food is an important consideration for future interventions aiming to assist this population in navigating the modern-day food system.
Developing a Dynamic Supervision Mechanism to Improve Construction Safety Investment Supervision Efficiency in China: Theoretical Simulation of Evolutionary Game Process
Authors: Gong Shitao , Gao Xin , Li Zhou , Chen Linyan
Published: March 2021

The construction industry suffers from poor safety performance caused by the joint effect of insufficient safety investment by contractors and inefficient safety supervision by the government because of the information gap between the two sides. The present study aims to put forward a new pathway to improve safety investment supervision efficiency and analyze the decision-making interactions of stakeholders under this new pathway. For this purpose, this study establishes a safety investment information system to eliminate the information gap between the government and contractors for construction projects in China and further develops a dynamic safety investment supervision mechanism based on this. Evolutionary game theory is used to describe the decision-making interactions among stakeholders under the current static supervision mechanism and the dynamic supervision mechanism proposed in this research. Moreover, system dynamics is adopted to simulate the evolutionary game process and analyze the supervision effect and equilibrium state of different supervision mechanisms. The results reveal that the proposed safety investment information system could facilitate the transition of the supervision mode from static to dynamic; the evolutionarily stable strategy does not exist in the current static penalty scenario; and the dynamic supervision mechanism that correlates penalties with contractors’ unlawful behavior probability can restrain the fluctuation of the evolutionary game model effectively and the players’ strategy choices gradually stabilize in the equilibrium state. The results validate the effectiveness of the proposed dynamic supervision mechanism in improving supervision efficiency. This study not only contributes to the literature on safety supervision policy-making but also helps to improve supervision efficiency in practice.
Evaluation of Service-Learning in Project WeCan under COVID-19 in a Chinese Context
Authors: Leung Hildie , Shek Daniel T. L. , Dou Diya
Published: March 2021

Service-learning is a widely adopted educational pedagogy and philosophy. With the support from the Wharf (Holdings) Limited (Group), service-learning was conducted in the “Project WeCan” in Hong Kong. Prior to COVID-19, traditional service-learning was implemented with students learning in the classroom and applying their knowledge and skills to the community through providing direct face-to-face service. With the COVID-19 outbreak in the 2019–20 academic year, school lockdown measures appeared. Students had to learn online and to design and implement service offsite. As the impacts of this rapid shift in paradigm remain unknown, this study examined changes in university students using a pretest–posttest design (n = 124) and perceptions of service-learning experience via the subjective outcome evaluation design (n = 192) under COVID-19. The authors also investigated service recipients’ (n = 56) satisfaction with service activities they participated in during the pandemic. Both objective outcome evaluation and subjective outcome evaluation findings revealed that service providers (university students) and recipients (secondary school students) experienced benefits from the Project. Findings support the benefits of online service-learning in “Project WeCan” even during unprecedented times such as COVID-19.
Effects of Land-Use Change on the Community Structure of the Dung Beetle (Scarabaeinae) in an Altered Ecosystem in Southern Ecuador
Authors: Carrión-Paladines Vinicio , Fries Andreas , Muñoz Andrés , Castillo Eddy , García-Ruiz Roberto , Marín-Armijos Diego
Published: March 2021

This study evaluated the effects of land-use change (L-UCH) on dung beetle community structure (Scarabaeinae) in a disturbed dry ecosystem in southern Ecuador. Five different L-UCH classes were analyzed by capturing the dung beetle species at each site using 120 pitfall traps in total. To determine dung beetle abundance and diversity at each L-UCH, a general linear model (GLM) and a redundancy analysis (RDA) were applied, which correlated environmental and edaphic conditions to the community structure. Furthermore, changes in dung-producing vertebrate fauna were examined, which varied significantly between the different L-UCH classes due to the specific anthropogenic use or level of ecosystem disturbance. The results indicated that soil organic matter, pH, potassium, and phosphorus (RDA: component 1), as well as temperature and altitude (RDA: component 2) significantly affect the abundance of beetles (GLM: p value < 0.001), besides the food availability (dung). The highest abundance and diversity (Simpson’s index > 0.4, Shannon-Wiener index > 1.10) was found in highly disturbed sites, where soils were generally more compacted, but with a greater food supply due to the introduced farm animals. At highly disturbed sites, the species Canthon balteatus, Dichotomius problematicus, and Onthphagus confuses were found specifically, which makes them useful as bio-indicators for disturbed dry forest ecosystems in southern Ecuador.
Characteristics of Adult Patients with Idiopathic Retroperitoneal Fibrosis and Assessment of Risk of Relapse at Diagnosis
Authors: Razanamahery Jerome , Bouldoires Bastien , Humbert Sebastien , Bielefeld Philip , Fournier Veronique , Bonnotte Bernard , Blaison Gilles , Magy-Bertrand Nadine
Published: March 2021

Objectives: To compare adult patients’ characteristics suffering from idiopathic retroperitoneal fibrosis between “relapse-free” and relapsing patients at the diagnosis and identify factors associated with relapse at initial presentation. Methods: We conducted a retrospective multicentric study in four hospitals in Eastern France, from 1993 to 2020, of adult patients suffering from idiopathic retroperitoneal fibrosis. We analyzed clinical, biological, and radiological features at diagnosis and during a forty-month follow-up. Results: Of 47 patients suffering from retroperitoneal fibrosis, 21 patients had idiopathic retroperitoneal fibrosis. Among them, 13 experienced one or more relapses during follow-up. At diagnosis, clinical characteristics, relevant comorbidities, biological and radiological features were similar between groups. Smoking cessation seems associated with decreased relapse risk (p: 0.0624). A total of 8 patients developed chronic renal failure during follow-up. Ureteral infiltration at diagnosis was associated with evolution to chronic renal failure (p: 0.0091). Conclusion: No clinical, biological, or radiological features could predict relapse at retroperitoneal fibrosis diagnosis, but smoking cessation may prevent relapse.
Modulation of the Dental Pulp Stem Cell Secretory Profile by Hypoxia Induction Using Cobalt Chloride
Authors: Bhandi Shilpa , Al Kahtani Ahmed , Mashyakhy Mohammed , Alsofi Loai , Maganur Prabhadevi C. , Vishwanathaiah Satish , Testarelli Luca , Del Giudice Andrea , Mehta Deepak , Vyas Nishant , Patil Vikrant R. , Raj A. Thirumal , Patil Shankargouda
Published: March 2021

The action of stem cells is mediated by their paracrine secretions which comprise the secretory profile. Various approaches can be used to modify the secretory profile of stem cells. Creating a hypoxic environment is one method. The present study aims to demonstrate the influence of CoCl2 in generating hypoxic conditions in a dental pulp stem cell (DPSCs) culture, and the effect of this environment on their secretory profile. DPSCs that were isolated from human permanent teeth were characterized and treated with different concentrations of CoCl2 to assess their viability by an 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and proliferation by a cell counting kit (CCK)-8 assay. The gene expression level of hypoxia-inducible factor 1-alpha (HIF-1α) was analyzed by quantitative real time polymerase chain reaction (qRT-PCR) to demonstrate a hypoxic environment. Comparative evaluation of the growth factors and cytokines were done by cytometric bead array. Gene expression levels of transcription factors OCT4 and SOX2 were analyzed by qRT-PCR to understand the effect of CoCl2 on stemness in DPSCs. DPSCs were positive for MSC-specific markers. Doses of CoCl2, up to 20 µM, did not negatively affect cell viability; in low doses (5 µM), it promoted cell survival. Treatment with 10 µM of CoCl2 significantly augmented the genetic expression of HIF-1α. Cells treated with 10 µM of CoCl2 showed changes in the levels of growth factors and cytokines produced. It was very evident that CoCl2 also increased the expression of OCT4 and SOX2, which is the modulation of stemness of DPSCs. A CoCl2 treatment-induced hypoxic environment modulates the secretory profile of DPSCs.
The Effects of Terrain Factors and Cultural Landscapes on Plateau Forest Distribution in Yushu Tibetan Autonomous Prefecture, China
Authors: Cui Naixin , Zou Huiting , Zhang Moshi , Guo Luo
Published: March 2021

The Yushu Tibetan Autonomous Prefecture is a typical Tibetan plateau area, and its ecological environment is very fragile. It is necessary to explore the terrain and cultural factors for the protection of the local ecological environment. We mainly investigated and quantified the effect of terrain factors and two typical plateau cultural landscapes (temples and villages) on the spatiotemporal variation characteristics of four types of forest landscape in the Yushu Tibetan Autonomous Prefecture from 1990 to 2015 using remote sensing (RS) and geographic information system (GIS) technology. The results showed that, under the influence of terrain factors, forest landscapes were only distributed in places with an altitude of 5055 meters above sea level (masl) to 6300 masl, with a slope of 0–27°, and the largest distribution area was shrubbery. The area of the forest decreased with the increase in altitude, and it first rose and then decreased with the increase in slope. Regression analysis results showed that the influence of altitude on closed forest land and open forest land followed a polynomial function, while that on shrubbery followed a logarithmic function, and the impact of slope on the three forest landscapes followed the amplitude version of a Gaussian peak function. Considering cultural factors, temples and villages did not determine the forest distribution in the same way as natural factors do, but they motivated the amount of forest over spatiotemporal scales. Temples had a greater influence on forest protection than villages, and this positive impact was stronger within 6 km. The area of forest distributed around the temple accounts for more than 45.67% of the total forest area, and this area has not changed significantly in 25 years. In summary, altitude and slope affect the natural distribution of the forest, and temples affect the scale of forest distribution. These results reveal the impact of terrain factors and cultural landscapes on forest distribution and could motivate an even more effective management for sustainable forest development.
Isolated Arthroscopic Lateral Retinacular Release for Lateral Patellar Compression Syndrome
Authors: Migliorini Filippo , Lüring Christian , Eschweiler Jörg , Baroncini Alice , Driessen Arne , Spiezia Filippo , Tingart Markus , Maffulli Nicola
Published: March 2021

Introduction: Evidence concerning the role of isolated lateral retinacular release (LRR) for lateral patellar compression syndrome (LPCS) dates back at least three decades. Appropriate indications, execution and outcomes still remain unclear and controversial. The present investigation analyzed the midterm result of isolated and arthroscopic LRR for LPCS in a cohort of patients who underwent such procedure at our institution. Material and methods: Patients undergoing isolated arthroscopic LRR for LPCS were identified retrospectively from our electronic database. All procedures were performed by two experienced surgeons. Patients with bony and/or soft tissues abnormalities, patellofemoral instability, moderate to severe chondral damage were not included. Patients with previous surgeries were not included, as were those who underwent combined interventions. Clinical scores and complications were recorded. Results: 31 patients were recruited in the present investigation. The mean follow-up was 86.0 ± 22.8 months. The mean age of the patients at the index operation was 34.2 ± 13.1 years. A total 55% (17 of 31) were women, and 58% (18 of 31) had involved the right knee. The mean hospitalization length was 3.5 ± 1.4 days. At a mean follow-up of 86.0 ± 22.8 months, the numeric rating scale (NRS) was 1.2 ± 0.8, the Kujala score was 91.3 ± 11.3, the Lysholm score was 93.1 ± 15.0, and the Tegner score was 5.0 ± 1.8. At the latest follow-up, 9 of 31 (29.0%) of patients experienced compilations. One patient (3.2%) had a post-operative hemarthrosis which was managed conservatively. Six patients (19.4%) reported a persistent sensation of instability, without signs of patellar dislocation or subluxation. One patient underwent an arthroscopic meniscectomy, and another patient an anterior cruciate ligament (ACL) reconstruction. Conclusion: isolated arthroscopic lateral retinacular release for lateral patellar compression syndrome is feasible and effective, achieving satisfying results at more than seven years following the procedure.