Potensi Lecanicillium lecanii untuk Pengendalian Helopeltis antonii pada Tanaman Teh
Authors: Indriati Gusti , Samsudin . , Amaria Widi 
Published: September 2016

<p><em>Lecanicillium lecanii </em>merupakan jamur entomopatogen yang berpotensi untuk pengendali hama pengisap daun teh <em>Helopeltis antonii</em>. Penelitian bertujuan mengetahui potensi <em>L. lecanii </em>dalam mengendalikan <em>H. antonii</em> pada tanaman teh. Penelitian dilakukan di Laboratorium Proteksi Tanaman Balai Penelitian Tanaman Industri dan Penyegar (Balittri), mulai Januari sampai Oktober 2014. Pengujian terdiri atas uji infektivitas dan antifedansi <em>L. lecanii</em> terhadap nimfa <em>H. antonii</em> serta uji kompatibilitas dengan insektisida nabati secara <em>in vitro</em>, menggunakan rancangan acak lengkap (RAL). Uji infektivitas dan antifedansi dilakukan pada empat kerapatan, yaitu 10<sup>6</sup>, 10<sup>7</sup>, 10<sup>8</sup>, 10<sup>9 </sup>konidia/ml dan kontrol. Setiap perlakuan uji infektivitas diulang empat kali dengan variabel pengamatan adalah mortalitas dan perkembangan nimfa, sedangkan uji antifedansi diulang sepuluh kali dengan variabel pengamatan adalah persentase pengurangan makan. Uji kompatibilitas dilakukan secara <em>in vitro</em> dengan serbuk <em>Piper retrofractum</em> (PR), <em>Tephrosia vogelii</em> (TV), dan <em>Annona squamosa</em> (AS), masing-masing 2,5%; 5,0%; 7,5%; dan kontrol, dan diulang empat kali. Hasil penelitian menunjukkan jamur <em>L. lecanii</em> mampu menginfeksi dan mematikan nimfa <em>H. antonii</em> sebesar 65% di laboratorium dan mengurangi makan sebesar 83,04%. Penambahan serbuk <em>A. squamosa</em> dan <em>T. vogelii</em> masing-masing 2,5%–7,5% tidak mempengaruhi pertumbuhan koloni <em>L. lecanii</em> pada media PDA, tetapi  serbuk <em>P. retrofractum</em> dengan konsentrasi yang sama secara nyata menghambat pertumbuhan <em>L. lecanii</em>. Serbuk <em>A. squamosa </em>dan<em> T. vogelii </em>kompatibel dengan<em> L. lecanii</em>.</p><p><strong>Kata kunci: </strong>Teh,<em> Helopeltis antonii, Lecanicillium lecanii,</em><em> </em>entomopatogen, insektisida nabati</p><p> </p><p>Lecanicillium lecanii<em> is an entomopathogenic fungus that potential to control tea mosquito bug </em>Helopeltis antonii<em>. The research aimed to determine the </em><em>potential of </em>L. lecanii<em> in controlling H. antonii in tea plant.</em><em> The research was conducted at the Laboratory of Plant Protection, Indonesian Industrial and Beverages Crops Research Institute (IIBCRI) Sukabumi, from January to October 2014. The study consisted of infectivity and antifeedancy of </em>L. lecanii<em> on </em>H. antonii<em> nymph</em><em>, as well as its in vitro compatibility with botanical insecticide, using completely randomized design (CRD). The infectivity and antifeedancy tests of </em>L. lecanii<em> on </em>H. antonii<em> nymph were carried out using</em><em> 4 densities: 10<sup>6</sup>, 10<sup>7</sup>, 10<sup>8</sup>, 10<sup>9</sup> conidia/ml, and control. Each treatment of infectivity test was repeated 4 times with observed variables were mortality and development of nymphs. Meanwhile, antifeedancy test was repeated 10 times with the observed variable was percentage of feeding reduction. The in vitro compatibility test of </em>L. lecanii<em> </em><em>was done by using </em>Piper retrofractum<em> (PR), </em>Tephrosia vogelii<em> (TV) and </em>Annona squamosa<em> (</em><em>AS) powder with the concentration of 2.5%, 5.0%, 7.5%, respectively and control, which repeated 4 times. The results showed that </em>L. lecanii<em> capable </em><em>to infect and kill </em>H. antonii<em> nymph </em><em>at about 65% in laboratory and reduce feeding about 83.04%. Addition of </em>A. squamosa<em> and </em>T. vogelii<em> powder </em><em>at 2.5%–7.5% concentration were not significantly affecting the growth of </em>L. lecanii<em> colon</em><em>ies on PDA media. On the other hand, addition of </em>P. retrofractum<em> powder with the same concentration was considerably inhibiting</em><em> the growth of </em>L. lecanii<em>. This result demonstrates that </em>A. squamosa<em> and </em>T. vogelii<em> powder are compatible with L. lecanii.</em></p>