bluuebunny/crossref_metadata_2025 huggingface dataset:
Tissue culture coupled with a gas exchange system offers new perspectives on phenotyping the developmental biology of Solanum lycopersicum L. cv. ‘MicroTom’
Authors: Pepe Marco , Marie Telesphore R. J. G. , Leonardos Evangelos D. , Hesami Mohsen , Rana Naheed , Jones Andrew Maxwell Phineas , Grodzinski Bernard
Published: November 2022

Solanum lycopersicum L. cv. ‘Microtom’ (MicroTom) is a model organism with a relatively rapid life cycle, and wide library of genetic mutants available to study different aspects of plant development. Despite its small stature, conventional MicroTom research often requires expensive growth cabinets and/or expansive greenhouse space, limiting the number of experimental and control replications needed for experiments, and can render plants susceptible to pests and disease. Thus, alternative experimental approaches must be devised to reduce the footprint of experimental units and limit the occurrence problematic confounding variables. Here, tissue culture is presented as a powerful option for MicroTom research that can quell the complications associated with conventional MicroTom research methods. A previously established, non-invasive, analytical tissue culture system is used to compare in vitro and conventionally produced MicroTom by assessing photosynthesis, respiration, diurnal carbon gain, and fruit pigments. To our knowledge, this is the first publication that measures in vitro MicroTom fruit pigments and compares diurnal photosynthetic/respiration responses to abiotic factors between in vitro and ex vitro MicroTom. Comparable trends would validate tissue culture as a new benchmark method in MicroTom research, as it is like Arabidopsis, allowing replicable, statistically valid, high throughput genotyping and selective phenotyping experiments. Combining the model plant MicroTom with advanced tissue culture methods makes it possible to study bonsai-style MicroTom responses to light, temperature, and atmospheric stimuli in the absence of confounding abiotic stress factors that would otherwise be unachievable using conventional methods.
Variations in genetic diversity in cultivated Pistacia chinensis
Authors: Han Biao , Zhang Ming-Jia , Xian Yang , Xu Hui , Cui Cheng-Cheng , Liu Dan , Wang Lei , Li De-Zhu , Li Wen-Qing , Xie Xiao-Man
Published: November 2022

Identification of the evolution history and genetic diversity of a species is important in the utilization of novel genetic variation in this species, as well as for its conservation. Pistacia chinensis is an important biodiesel tree crop in China, due to the high oil content of its fruit. The aim of this study was to uncover the genetic structure of P. chinensis and to investigate the influence of intraspecific gene flow on the process of domestication and the diversification of varieties. We investigated the genetic structure of P. chinensis, as well as evolution and introgression in the subpopulations, through analysis of the plastid and nuclear genomes of 39 P. chinensis individuals from across China. High levels of variation were detected in the P. chinensis plastome, and 460 intraspecific polymorphic sites, 104 indels and three small inversions were identified. Phylogenetic analysis and population structure using the plastome dataset supported five clades of P. chinensis. Population structure analysis based on the nuclear SNPs showed two groups, clearly clustered together, and more than a third of the total individuals were classified as hybrids. Discordance between the plastid and nuclear genomes suggested that hybridization events may have occurred between highly divergent samples in the P. chinensis subclades. Most of the species in the P. chinensis subclade diverged between the late Miocene and the mid-Pliocene. The processes of domestication and cultivation have decreased the genetic diversity of P. chinensis. The extensive variability and structuring of the P. chinensis plastid together with the nuclear genomic variation detected in this study suggests that much unexploited genetic diversity is available for improvement in this recently domesticated species.
Flavonoids and Devosia sp SL43 cell-free supernatant increase early plant growth under salt stress and optimal growth conditions
Authors: Shah Ateeq , Subramanian Sowmyalakshmi , Smith Donald L.
Published: November 2022

Salt stress is a major threat to modern agriculture, significantly affecting plant growth and yield, and causing substantial economic losses. At this crucial time of increasing climate change conditions, soil salinity will continue to develop and become an even more serious challenge to crop agriculture. Hence, there is a pressing need for sustainable techniques in agricultural production that could meet the dual challenges of crop productivity and environmental instability. The use of biostimulants in agricultural production has greatly influenced plant health and global food production. In particular, the application of bioactive materials produced by beneficial microbes is becoming a common practice in agriculture and provides numerous benefits to plant growth and resistance to stressful conditions. In this research two biostimulants; a type of plant secondary metabolite (flavonoids) and a microbe-based material (CFS: Cell-Free Supernatant) containing active compounds secreted by a novel bacterial strain isolated from Amphecarpaea bracteata root nodules (Devosia sp - SL43), have been utilized to improve the growth and stress resistance of two major oil seed crops; canola and soybean, under optimal and salt stress conditions. Our findings suggested significant improvements in crop growth of canola and soybean following the application of both biostimulants. Under optimal growth conditions, soybean growth was significantly affected by foliar spray of flavonoids with increases in shoot fresh and dry weight, and leaf area, by 91, 99.5, and 73%, respectively. However, soybean growth was unaffected by flavonoids under salt stress. In contrast, CFS with a meaningful capacity to mitigate the negative effects of salinity stress improved soybean shoot fresh biomass, dry biomass, and leaf area by 128, 163 and 194%, respectively, under salt stress conditions. Canola was less responsive to both biostimulants, except for canola root variables which were substantially improved by flavonoid spray. Since this was the first assessment of these materials as foliar sprays, we strongly encourage further experimentation to confirm the findings reported here and to determine the full range of applicability of each of these potential technologies.
Green synthesis and application of GO nanoparticles to augment growth parameters and yield in mungbean (Vigna radiata L.)
Authors: Mirza Faisal Shafiq , Aftab Zill-e-Huma , Ali Muhammad Danish , Aftab Arusa , Anjum Tehmina , Rafiq Hamza , Li Guihua
Published: November 2022

Plant growth promotion has long been a challenge for growers all over the world. In this work, we devised a green nanomaterial-assisted approach to boost plant growth. It has been reported that carbon nanomaterials are toxic to plants because they can inhibit the uptake of nutrients if employed in higher concentrations, however this study shows that graphene oxide (GO) can be used as a regulator tool to improve plant growth and stability. Graphene oxide in different concentrations was added to the soil of mungbean. It is proved that when a suitable amount of graphene oxide was applied, it had a good influence on plant growth by enhancing the length of roots and shoots, number of leaves, number of root nodules per plant, number of pods, and seeds per pod. We presume that the use of bio-fabricated graphene oxide as a strategy would make it possible to boost both plant growth and the significant increase in the number of seeds produced by each plant.
SmDXS5, acting as a molecular valve, plays a key regulatory role in the primary and secondary metabolism of tanshinones in Salvia miltiorrhiza
Authors: Zhang Da-chuan , Luo Ling-long , Wang Zhi-rong , Xu Wen-juan , Li Jun-ling , Tan Shu-ting , Wu Jia-hui , Li Yan , Zhang Chi , Liang Chen , Wang Xue-yong
Published: November 2022

Red sage, the dry root and rhizome of the herbaceous plant Salvia miltiorrhiza Bunge, is widely used for treating various diseases. The low content of tanshinones (terpenoids) has always restricted development of the S. miltiorrhiza industry. Here, we found that SmDXS5, a rate-limiting enzyme-coding gene located at the intersection of primary and secondary metabolism, can effectively change the transcription level and secondary metabolome profile of hairy roots of S. miltiorrhiza, and significantly increase the content of tanshinones. Agrobacterium rhizogenes was used to infuse S. miltiorrhiza explants, and hairy roots of S. miltiorrhiza expressing the SmDXS5 gene were obtained successfully. We identified 39 differentially accumulated metabolites (DAMs) by metabolomics based on ultra-high performance liquid chromatography quadrupole exactive mass spectrometry and multivariate statistics. These DAMs might be key metabolites of SmDXS5 gene regulation. RNA sequencing was used to compare gene expression between the hairy roots of the SmDXS5 overexpressing group and the blank control (BC) group. Compared with the BC group, 18,646 differentially expressed genes were obtained: 8994 were upregulated and 9,652 downregulated. The combined transcriptome and metabolome analyses revealed that the mevalonate and methylerythritol phosphate pathways and synthase gene expression levels in the SmDXS5 overexpressing group were upregulated significantly, and the accumulation of tanshinone components was increased significantly, which promoted the process of glycolysis and promoted the transformation of carbohydrates to secondary metabolism. Moreover, the expression of SmPAL, the first rate-limiting enzyme gene of the phenylpropane pathway, decreased, reducing the accumulation of phenolic acid, another secondary metabolite. Therefore, SmDXS5 can be defined as a ‘valve’ gene, mainly responsible for regulating the distribution of primary and secondary metabolic flow of tanshinones in S. miltiorrhiza, and for other secondary metabolic pathways. The discovery of SmDXS5 and its molecular valve function in regulating primary and secondary metabolism will provide a basis for the industrial production of tanshinone components, and cultivation of high quality S. miltiorrhiza.
Regulatory non-coding RNAs: Emerging roles during plant cell reprogramming and in vitro regeneration
Authors: Cordeiro Daniela , Canhoto Jorge , Correia Sandra
Published: November 2022

Plant regeneration is a well-known capacity of plants occurring either in vivo or in vitro. This potential is the basis for plant micropropagation and genetic transformation as well as a useful system to analyse different aspects of plant development. Recent studies have proven that RNA species with no protein-coding capacity are key regulators of cellular function and essential for cell reprogramming. In this review, the current knowledge on the role of several ncRNAs in plant regeneration processes is summarized, with a focus on cell fate reprogramming. Moreover, the involvement/impact of microRNAs (miRNAs), long non-coding RNAs (lncRNAs) and small-interfering RNAs (siRNAs) in the regulatory networks of cell dedifferentiation, proliferation and differentiation is also analysed. A deeper understanding of plant ncRNAs in somatic cell reprogramming will allow a better modulation of in vitro regeneration processes such as organogenesis and somatic embryogenesis.
Nickel toxicity alters growth patterns and induces oxidative stress response in sweetpotato
Authors: Kumar Sunjeet , Wang Mengzhao , Liu Yi , Fahad Shah , Qayyum Abdul , Jadoon Sultan Akbar , Chen Yanli , Zhu Guopeng
Published: November 2022

Nickel (Ni) contaminated soil is a persistent risk to plant growth and production worldwide. Therefore, to explore the Ni toxicity levels in sweetpotato production areas, we investigated the influence of different Ni treatments (0, 7.5, 15, 30, and 60 mg L-1) for 15 days on phenotype, Ni uptake, relative water content, gas exchange, photosynthetic pigments, oxidative stress, osmolytes, antioxidants, and enzymes of sweetpotato plants. The results presented that Ni at higher levels (30 and 60 mg L-1) substantially reduced growth, biomass, and root morphological traits. The Pearson correlation analysis suggested that Ni toxicity causes oxidative injuries as persistent augmentation of hydrogen peroxide (H2O2) and malonaldehyde (MDA) and reduced RWC, gas exchange, and photosynthetic pigment. Furthermore, this study revealed that sweetpotato could tolerate moderate Ni treatment (up to 15 mg L-1) by reducing oxidative stress. The results also indicated that the increase in the activities of mentioned osmolytes, antioxidants, and enzymes is not sufficient to overcome the higher Ni toxicity. Based on these results, we suggest using low Ni-contaminated soil for better growth of sweetpotato and also could be used as a phytoremediator in moderate Ni-contaminated soil.
Quantitative trait locus mapping and improved resistance to sclerotinia stem rot in a backbone parent of rapeseed (Brassica napus L.)
Authors: Zhang Xiaohui , Li Xiang , Li Huining , Wang Zhuanrong , Xia Rui , Hu Jin , Wang Pengfei , Zhou Xianming , Wan Lili , Hong Dengfeng , Yang Guangsheng
Published: November 2022

There are three main challenges to improving sclerotinia stem rot (SSR) resistance in rapeseed (Brassica napus L.). First, breeding materials such as the backbone parents have not been extensively investigated, making the findings of previous studies difficult to directly implement. Second, SSR resistance and flowering time (FT) loci are typically linked; thus, use of these loci requires sacrifice of the rapeseed growth period. Third, the SSR resistance loci in susceptible materials are often neglected, thereby reducing the richness of resistant resources. This study was conducted to investigate the stem resistance, disease index, and FT of a doubled haploid population consisting of 151 lines constructed from the backbone parent 19514A and conventional rapeseed cultivar ZY50 within multiple environments. Quantitative trait locus (QTL) mapping revealed 13 stem resistance QTLs, 9 disease index QTLs, and 20 FT QTLs. QTL meta-analysis showed that uqA04, uqC03.1, and uqC03.2 were repeatable SSR resistance QTLs derived from different parents but not affected by the FT. Based on these three QTLs, we proposed a strategy for improving the SSR resistance of 19514A and ZY50. This study improves the understanding of the resistance to rapeseed SSR and genetic basis of FT and demonstrates that SSR resistance QTLs can be mined from parents with a minimal resistance level difference, thereby supporting the application of backbone parents in related research and resistance improvement.
Establishment and optimization of a new model organism to study early land plant evolution: Germination, cultivation and oospore variation of Chara braunii Gmelin, 1826
Authors: Holzhausen Anja , Stingl Nora , Rieth Sophie , Kühn Christine , Schubert Hendrik , Rensing Stefan Andreas
Published: November 2022

For studying land plant evolution, the establishment and optimization of model organisms representing streptophytic algae, sister to land plants, is essential. Long-term cultivation experiments withChara brauniiS276 were performed over 8 years, since 4 years (Nov. 2018) under constant conditions. Additionally, short-term experiments for optimization of culture conditions were performed with three strains ofC. braunii(S276, NIES-1604 and Lausiger Teiche, LaT-2708). Germination success after application of sterilization agents, addition of gibberellic acid and under different incubation conditions with respect to pre-treatment, irradiance regime and substrate was investigated in order to develop protocols for generative cultivation of at least unialgal cultures. The resulting cultivation protocols forC. brauniiS276, allowing maintenance of vegetative as well as generative cultures are presented in detail, including protocols for germination induction and growth of sterilized and unsterilized oospores.
Crowds, leaders, and epidemic psychosis: The relationship between crowd psychology and elite theory and its contemporary relevance
Authors: Damele Giovanni
Published: November 2022

The influence of the authors of the so-called “crowd psychology” on the conventional interpretation of the “irrationality” of the masses in political life has been widely recognized. More recently, Ernesto Laclau has underlined the influence of this tradition on the liberal-democratic views on direct, mass democracy. This conventional interpretation may be reconstructed starting from different intellectual traditions: crowd psychology, properly speaking, its complementary, the “classical” elite theory, and the influence of the positivist analysis of the “criminal” and “psychopathological” behaviors of the masses. However, this influence, far from being confined to the liberal distaste for mass democracy, has been relevant not only for the fascist regimes of the 20th century but also for the contemporary right-wing populist interpretation of the role of the masses in political life, in spite of its purported antielitism.
Attitudes and perceptions of teachers toward mental health literacy: A case of Odzi High School, Mutare District, Zimbabwe
Authors: Sibanda Theresi , Sifelani Innocent , Kwembeya Maurice , Matsikure Memory , Songo Sifikile
Published: November 2022

In dealing with adolescent mental health, teachers and school institutions have a critical role in minimizing the challenges of mental health. However, the success of such a measure is dependent on teachers’ attitudes and perceptions of mental health literacy. Adolescent mental health disorders have become a major global concern with heightened increases and prevalence in secondary schools. In Zimbabwe, a dearth of knowledge exists in understanding the perceptions and attitudes of teachers in maximizing student mental health and positive educational outcomes. The aim of the research was to understand the attitudes and perceptions of teachers toward mental health literacy. Using a qualitative approach to understanding attitudes and perceptions, a case study was carried out at Odzi High School. A total of 12 teachers were purposively sampled for data generation on knowledge, attitudes, perceptions, and strategies that can be used to inform adolescent mental health. Data were collected using semi-structured interview(s). Emergent themes of the study were knowledge, stigma, training, and development. Using thematic analysis, the data showed that there was generalized knowledge of mental health and there were disparities in effective and efficient responses to gender sensitivity issues. Additionally, this knowledge was not put into practice because of attitudes and perceptions toward mental health. Recommendations from the study include developing of mental health training for teachers, equipping teachers with skills in psychosocial support in schools’ mental health, and enabling teachers to be able to link adolescents to appropriate care within the school and community-based settings. Teachers have the responsibility of promoting the wellbeing of the students, thus minimizing the adverse effects. Outcomes for positive mental health may be the result of teachers’ informed perceptions and positive attitudes on mental health literacy.
“If it weren’t for COVID-19…”: Counterfactual arguments influence support for climate change policies via cross-domain moral licensing or moral consistency effects
Authors: Bertolotti Mauro , Valla Luca Guido , Catellani Patrizia
Published: November 2022

In two studies, we investigated whether counterfactual messages (i.e., “If… then…”) on the economic costs of past public policies influence support for future climate change policies. In Study 1, we tested whether the effect of upward counterfactual messages depended on their referring (or not) to the COVID-19 pandemic. Results showed lower support for a future climate change policy when the past expenses evoked by the upward counterfactual messages were attributed to COVID-19. In Study 2, we combined upward counterfactuals with downward counterfactuals presenting past economic efforts to deal with the COVID-19 pandemic as a moral credit. Results showed that exposure to downward counterfactuals decreased support for climate change policies among participants with low endorsement of anti-COVID-19 measures, whereas it increased support among participants with high endorsement. Discussion focuses on the conditions under which counterfactual communication may activate cross-dimensional moral licensing or moral consistency effects, influencing support for climate change policies.
Social media use, social identification and cross-cultural adaptation of international students: A longitudinal examination
Authors: Gaitán-Aguilar Leonor , Hofhuis Joep , Bierwiaczonek Kinga , Carmona Carmen
Published: November 2022

The mobility experience is an important life event for international students, and achieving successful psychological and sociocultural adaptation is crucial for this experience to be positive. Through a three-wave longitudinal study among international students enrolled at universities in Spain, Portugal, and Poland (n = 233), we examined the relationships between social media use, social identification, and (sociocultural and psychological) adaptation across time. Results of cross lagged panel modeling (CLPM) showed that social media contact with home nationals predicted greater identification with this group. Social media contact with host country nationals predicted poorer adaptation. Social media contact with other international students did not show any effects, while identification with this group predicted better adaptation. Our results point to the dynamic nature of the adaptation process, showing that the role of social media use and identification targeted at different social groups may play different roles than was previously found in cross-sectional research.
Processing stage flexibility of the SNARC effect: Task relevance or magnitude relevance?
Authors: Xiang Xinrui , Yan Lizhu , Fu Shimin , Nan Weizhi
Published: November 2022

Previous studies have shown that the processing stage of the spatial-numerical association of response codes (SNARC) effect is flexible. Two recent studies used the same experimental paradigm to check whether the SNARC effect occurred in the semantic-representation stage but reached contradictory conclusions, showing that the SNARC effect was influenced by a magnitude Stroop effect in a magnitude comparison task but not by a parity Stroop effect in a parity judgment task. Those two studies had two distinct operational factors: the task type (magnitude comparison task or parity judgment task, with the numerical magnitude information task-relevant or task-irrelevant) and the semantic representation stage-related interference information (magnitude or parity Stroop effect, with the interference information magnitude-relevant or magnitude-irrelevant). To determine which factor influenced the SNARC effect, in the present study, the Stroop effect was switched in the two tasks based on the previous studies. The findings of four experiments consistently showed that the SNARC effect was not influenced by the parity Stroop effect in the magnitude comparison task but was influenced by the magnitude Stroop effect in the parity judgment task. Combined with the results of those two contradictory studies, the findings indicated that regardless of the task type or the task relevance of numerical magnitude information, magnitude-relevant interference information was the primary factor to affect the SNARC effect. Furthermore, a two-stage processing model that explained the observed flexibility of the SNARC effect was proposed and discussed.
Research on the dissemination of celebrities’ opinions based on speech act theory and potential category analysis
Authors: Li Xinhe , Ju Chunhua , Li Ke , Wang Chenyu , Li Chuang , Yu Jie
Published: November 2022

With the increasingly prominent role of social media in the timeliness and sharing of information dissemination, more and more research has focused on how to further improve user stickiness through social media. However, there is little consideration of the impact of celebrities’ views on user behavior in social media. The main goal of this paper is to study the influence of celebrity language style on user communication and opinions dissemination. First, it analyzes the language style characteristics of celebrities’ opinions and conducts cross-influence analysis between celebrity language style characteristics and user communication characteristics. Based on speech act theory, this study studies the influence of different language styles of celebrity Microblog on users’ communication behavior and then builds a potential category analysis model to subdivide the views of celebrities. The results show that (1) Positive expression is the most common language style element combination of celebrities, and it also shows the most effective communication effect. This shows that users like to see celebrities show an active and positive side to the outside world, can analyze external things, and express their own opinions on these contents; (2) The combination of positive emotion, external attention, and analysis can produce the best communication effect; (3) The emotion of celebrities’ opinions will affect the communication emotion of users to a certain extent, and the communication of users will have the development trend of reducing positive emotions and increasing negative emotions. Therefore, positive guidance and the dissemination of positive energy are more needed on public social platforms to minimize or avoid the dissemination of negative emotions.
Using strengths to attack weaknesses – The effect of comparative advertising on purchasing intention of green products
Authors: Ni Kuocheng , Lin Yanfeng , Ye Shenghong , Lin Zhiheng , Liu Yunxiao
Published: November 2022

Firms increasingly use comparative advertising in green marketing to convey information of green products to consumers, but there is still a lack of research on the effect and mechanism of comparative advertising in the green products field. Across four experimental studies, we show that comparative advertising facilitates consumers’ purchase intention of green products (PIGP), because comparative advertising lead to higher perceived diagnosticity of Information. Yet, comparative advertising does not always bring high intention to buy green products. When using egoistic appeals, the perceived diagnosticity of information and purchase intention of green products were higher in comparative advertising than in non-comparative advertising. When utilizing altruistic appeals, there was no significant difference between the two kinds of advertising. In addition, individual differences of consumers also affect the effect of comparative advertising. The positive effect of comparative advertising on the purchase intention of green products is weakened for consumers with high green involvement. Our findings advance existing knowledge about the use of comparative advertising in green marketing and provide enlightening suggestions for how firms can promote consumers to buy green products.
Values and attitude certainty: The case for attitude clarity and correctness
Authors: Blankenship Kevin L. , Kane Kelly A. , Machacek Marielle G.
Published: November 2022

Three studies examined how the perception that one’s attitudes are based in values affects attitude clarity and correctness. Specifically, perceiving that one’s attitude is based in important values increases attitude clarity (the subjective sense that one knows one’s attitude) but not attitude correctness (the subjective sense that the attitude is correct). To test this, participants read a counterattitudinal message and were given feedback about the basis of their attitude. Relative to participants who learned that their attitudes were weakly based in values, participants who were told that their attitudes were strongly based in values reported greater attitude clarity than correctness (Study 1). Similarly, increases in attitude clarity from having an attitude based in values increased the perception that participants effortfully processed the message (Studies 2 and 3), the belief that participants more successfully resisted the message, and participants’ intentions to act on the attitude.
Abusive leadership: A moderated-mediation through leader-member exchange and by organizational politics
Authors: Drory Amos , Shkoler Or , Tziner Aharon
Published: November 2022

Abusive leadership has been shown to have adverse consequences for both the employees and the organization. In the current paper, the impacts of such a leadership style on workers’ turnover intentions (TIs), counterproductive work behaviors (CWBs) and organizational citizenship behaviors (OCBs) are investigated through a dyadic lens—the mediation of leader-member exchange (LMX). Furthermore, when the workplace atmosphere is also tainted by high level of perceived organizational politics (POP) (as a moderator), these relationships deepen and and/or change (for the worse). To test the moderated-mediation research model, an online sample of 619 participants was obtained. The results support an interesting moderated-mediation of LMX by POP. Theoretical and practical implications, limitations and future research suggestions are discussed.
Child temperament and child-teacher relationship quality: Implications for children’s emotional functioning during preschool period
Authors: Susa-Erdogan Georgiana , Benga Oana , Albu-Răduleț Mihaela , Macovei Teodora
Published: November 2022

Although, in the last years several studies have moved beyond analyzing the role of mother–child relationship in the association between child temperament and child emotional functioning, our knowledge is still limited about which fine-grained temperamental components of child reactivity and self-regulation are associated with child-teacher relationship quality. Also, fewer studies have looked at the moderating role of child-teacher relationship in the association between child temperament and child internalizing/externalizing problems during early childhood. The present study examined the relation between components of child temperamental Negative Affectivity, Surgency, and Effortful Control and child-teacher relationship quality (i.e., closeness, conflict) in preschool children. In addition, our aim was to test the moderating effect of the child-teacher relationship on the association between temperament and internalizing and externalizing problems. One hundred Romanian preschoolers (55 boys, mean age = 4.04 years) participated in this study. Mothers assessed their child’s temperament by completing the Children’s Behavior Questionnaire and externalizing and internalizing problems with the Child Behavior Checklist. Child-teacher relationship quality was evaluated by children’s teachers using the Student-Teacher Relationship Scale. Our results revealed that teachers rated their relationship as less conflictual with children who were assessed by their mothers as better in shifting and focusing attention, enjoying situations involving low stimulus intensity and displaying higher levels of Shyness, Sadness and Activity Level. Moreover, higher levels of Discomfort were associated with more conflict and less closeness while emotional reactivity such as Sadness, Fearfulness, and Activity Level were positively associated with closeness. Teacher-child closeness was associated with three temperamental self-regulation factors in the expected direction, except inhibitory control. Furthermore, results revealed a statistically significant interaction between child temperamental Shyness and child-teacher closeness in the prediction of child internalizing problems. Thus, when child-teacher closeness was low, there was a significant and positive relationship between child temperamental Shyness and child internalizing problems. Results highlight the importance of child-teacher relationship quality in relation to child temperament and social–emotional development during preschool period.
The double-edged sword effect of argumentative scaffolding on group discussion in an adaptive discussion system
Authors: Gao Hongli , Xu Sheng , Yang Lei , Hu Xiangen
Published: November 2022

Group discussion is a common and important form of learning. The effectiveness of group discussion could be facilitated by the adaptive support of virtual agent. Argumentative knowledge construction is beneficial to learners’ acquisition of knowledge, but the effectiveness of argumentative scaffolding is not consistent in existing studies. In this study, a total of 64 college students (32 groups, two participants and one computer agent in each group) participated in the experiment and they were assigned to the experimental condition (16 groups) and the control condition (16 groups). In the control condition, the computer agent would give an idea from semantically different categories according to the automatic categorization of the current discussion. In the experimental condition, the computer agent provided argumentative scaffolding after giving diverse ideas to support participants’ deep processing. The argumentative scaffolding included two prompt questions, “do you agree with me?” and “could you give the reasons to support your viewpoint?.” The dependent variables were the interaction quality, network centrality, the breadth and depth of discussion, the self-reported of discussion effectiveness and the degree of change before and after the discussion. Findings revealed that compared with the control condition, the participants were more likely to discuss the keywords provided by the virtual agent and reported more comprehensive understanding of the discussion topic, but surveyed less ideas and interactions during the discussion under the argumentative condition. This study suggests that the argumentative scaffolding may have both positive and negative effect on the group discussion and it’s necessary to make a choice.
Research trends in social media addiction and problematic social media use: A bibliometric analysis
Authors: Pellegrino Alfonso , Stasi Alessandro , Bhatiasevi Veera
Published: November 2022

Despite their increasing ubiquity in people's lives and incredible advantages in instantly interacting with others, social media's impact on subjective well-being is a source of concern worldwide and calls for up-to-date investigations of the role social media plays in mental health. Much research has discovered how habitual social media use may lead to addiction and negatively affect adolescents' school performance, social behavior, and interpersonal relationships. The present study was conducted to review the extant literature in the domain of social media and analyze global research productivity during 2013–2022. Bibliometric analysis was conducted on 501 articles that were extracted from the Scopus database using the keywords social media addiction and problematic social media use. The data were then uploaded to VOSviewer software to analyze citations, co-citations, and keyword co-occurrences. Volume, growth trajectory, geographic distribution of the literature, influential authors, intellectual structure of the literature, and the most prolific publishing sources were analyzed. The bibliometric analysis presented in this paper shows that the US, the UK, and Turkey accounted for 47% of the publications in this field. Most of the studies used quantitative methods in analyzing data and therefore aimed at testing relationships between variables. In addition, the findings in this study show that most analysis were cross-sectional. Studies were performed on undergraduate students between the ages of 19–25 on the use of two social media platforms: Facebook and Instagram. Limitations as well as research directions for future studies are also discussed.
Understanding patient characteristics and medication prescriptions in children with mental health and neurodevelopmental disorders referred to a sleep clinic—A quality improvement/quality assurance analysis
Authors: Ipsiroglu Osman S. , Bhathella Juhi , Boldut Renee Paula , Elbe Dean , Hill Olivia , Keys Elizabeth , McWilliams Scout , Silvestri Rosalia , Wensley David F.
Published: November 2022

Introduction

Motivated by challenges faced in outpatient sleep services for mental health and neurodevelopmental disorders (MHNDD) during the COVID-19 clinical shutdown, a pan-Canadian/international working group of clinicians and social scientists developed a concept for capturing challenging sleep and wake behaviours already at the referral stage in the community setting.

Methods

In a quality improvement/quality assurance (QIQA) project, a visual logic model was the framework for identifying the multiple causes and possible interventions for sleep disturbances. Intake forms informed clinicians about situational experiences, goals/concerns, in addition to the questions from the Sleep Disturbances Scale for Children (SDSC), the ADHD Rating Scale-IV and medication history. Descriptive statistics were used to describe the sample.

Results

66% of the pilot study patients (n = 41) scored in the SDSC red domains (highest scoring) with highest sub-scores for insomnia (falling asleep 73%; staying asleep: 51%) and daytime somnolence (27%). A total of 90% of patients were taking at least one medication; 59% sleep initiation/sleep medications, 41% in combination with further non-stimulant medications, 9% with stimulants, 27% with antidepressants and 18% with antipsychotics. Polypharmacy was observed in 62% of all patients and in 73% of the ones medicated for sleep disturbances. Qualitative information supported individualisation of assessments.

Conclusion

Our intake process enabled a comprehensive understanding of patients’ sleep and wake profiles prior to assessment, at the referral stage. The high prevalence of insomnia in patients, combined with polypharmacy, requires special attention in the triaging process at the community level.
A history of childhood maltreatment is associated with altered DNA methylation levels of DNA methyltransferase 1 in maternal but not neonatal mononuclear immune cells
Authors: Mavioglu Rezan Nehir , Ramo-Fernandez Laura , Gumpp Anja M. , Kolassa Iris-Tatjana , Karabatsiakis Alexander
Published: November 2022

Childhood maltreatment (CM) is associated with alterations in DNA methylation (DNAm) especially in stress response genes. Due to the higher risk of overall health complications of individuals with a parental history of CM, intergenerational transmission of CM-associated DNAm changes has been investigated but remains unclear. In this study, we investigated if different severities of CM have any influence on the DNAm of DNA methyltransferase 1 (DNMT1), an important enzyme of the DNAm machinery, in immune and buccal cells of mother-newborn dyads. DNAm was assessed by mass spectrometry using immune cell DNA from mothers (N = 117) and their newborns (N = 113), and buccal cell DNA of mother-newborn dyads (N = 68 each). Mothers with a history of CM had lower mean methylation of DNMT1 in immune cells compared to the mothers without a CM history. CM status only influenced maternal DNMT1 gene expression when at least moderate CM was reported. Buccal cell DNAm was not associated with CM status. Maternal history of CM was not linked to any alterations in DNMT1 mean DNAm in any of the cell types studied in newborns. We conclude that the CM-associated alterations in DNMT1 DNAm might point to allostatic load and can be physiologically relevant, especially in individuals with more severe CM experiences, resulting in an activated DNA methylation machinery that might influence stress response genes. Our lack of significant findings in buccal cells shows the tissue-specific effects of CM on DNAm. In our sample with low to moderate maternal CM history, there was no intergenerational transmission of DNMT1 DNAm in newborns.
Psychopathology of psychiatric patients presenting autoantibodies against neuroglial antigens
Authors: Grenzer Insa Maria , Juhl Aaron Levin , Teegen Bianca , Fitzner Dirk , Wiltfang Jens , Hansen Niels
Published: November 2022

Background

Autoantibody-mediated psychiatric disorder is often difficult to diagnose as the clinical features of psychiatric disorder associated with neural autoantibodies are often similar. Thus, it is of major relevance to investigate whether psychopathology can differentiate between both disease entities as a biomarker and help us in searching for specific autoantibodies associated with psychiatric symptoms.

Methods

We enrolled 154 patients of the Department of Psychiatry and Psychotherapy of the University Medical Center Göttingen with psychopathology data and retrospectively evaluated their patient records using the classification systems AMDP (Arbeitsgemeinschaft für Methodik und Dokumentation in der Psychiatrie) and HiTOP (Hierarchical Taxonomy of Psychopathology).

Results

We identified 35 psychiatric patients revealing autoantibodies in their serum and/or cerebrospinal fluid (CSF) and 119 with no autoantibodies. Relying on the AMDP system, many more psychiatric patients with serum autoantibodies (51%) had problems with orientation than those without autoantibodies (32%) (p < 0.05). Furthermore, fewer psychiatric patients with serum autoantibodies exhibited a blunted affect (11.4 vs. 32.8%, p < 0.01) and affective rigidity (20 vs. 45%, p < 0.01). In particular, psychiatric patients presenting CSF autoantibodies (indicating an autoimmune symptomatic basis) experience more loss of vitality (5%) than those without autoantibodies (0%) (p < 0.05). Another interesting finding is that according to the AMDP classification, a manic syndrome is much more frequent in autoantibody-positive (8.6%) than autoantibody-negative psychiatric patients (0.8%) (p < 0.05). Another aspect is the more frequent occurrence of attention and memory deficits in patients with autoantibodies against intracellular targets compared with targets on the membrane surface.

Conclusion

Our findings indicate that neural autoantibodies in psychiatric patients could indicate a phenotype more often characterized by a manic syndrome, orientation disturbances within the cognitive spectrum, and fewer affect disturbances characterized by less blunted affect and not as seriously impaired feelings of vitality compared to controls. The novelty of our approach is the extensive autoantibody tests for various psychiatric syndromes in combination with a profound psychometric measurement with two different scales.
Internet behavior patterns of adolescents before, during, and after COVID-19 pandemic
Authors: Wu Qianying , Ren Qihuan , Zhong Na , Bao Juwang , Zhao Yan , Du Jiang , Chen Tianzhen , Zhao Min
Published: November 2022

Background

The outbreak of COVID-19 has affected the mental health of adolescents. To describe the Internet behavior-changing patterns of adolescents and to understand the impact of clinical features on changing patterns during the COVID-19 pandemic.

Materials and methods

We conducted a cross-sectional cohort study using data collected through online investigation in China. A total of 625 adolescents completed the online survey from May 15 to June 7, 2020. The adolescents were asked to retrospect to the Internet behaviors and game behaviors of three time periods as follows: before the COVID-19 outbreak in China, during the COVID-19 outbreak in China, and back to school. The clinical variables of the demographic data, family functionality, and emotional and behavioral symptoms were also collected. According to the Internet behaviors and game behaviors patterns across the three time periods, the subjects will be sub-grouped.

Results

Four Internet behavior-changing patterns during the COVID-19 was identified: (1) Continuous Normal Group (55.52%); (2) Normal to Internet Addiction Group (5.28%); (3) Internet Addiction to Normal Group (14.56%); and (4) Continuous Internet Addiction Group (24.64%). Years of education, academic score ranking, family functionality, and emotional and behavioral symptoms were different across the four groups. Proportions of game behaviors, scores of Strengths and Difficulties Questionnaire (SDQ), and SDQ subscale during the period before the COVID-19 outbreak were significant in predicting changing patterns.

Conclusion

The Internet behavior patterns of adolescents during the COVID-19 period were various. Clinical features before the COVID-19 pandemic may predict changing patterns. The heterogeneity in characteristics between different changing patterns should be considered when intervening in adolescents’ problematic Internet behavior.
The Inventory of Personality Organization: A valid instrument to detect the severity of personality dysfunction
Authors: Unoka Zsolt , Csáky-Pallavicini Krisztina , Horváth Zsolt , Demetrovics Zsolt , Maraz Aniko
Published: November 2022

Background and aims

In the eleventh revision of the International Classification of Diseases (ICD-11), the severity of personality dysfunction became the central dimension of personality disorder’s (PDs) definition, besides the trait domain qualifiers. Personality functioning, also known as personality organization (PO), is becoming an increasingly important concept in administering, predicting, and measuring severity and nature of personality disturbance. Otto Kernberg and his team developed several tools to measure personality impairment. The Inventory of Personality Organization (IPO) is a self-report rating scale for the measurement of PO. Aim of this study was to identify severity groups according to the level of PO and to explore their validity.

Materials and methods

A clinical sample of 118 patients was recruited from a 4-weeks in-patient cognitive psychotherapy program. Beside the IPO, Structured Clinical Interview for the fourth edition of the Diagnostic and Statistical Manual of Mental Disorders, (DSM-IV.) Axis I and II, Symptom Check List-90 (SCL-90), State-Trait Anger Expression Inventory and Dissociative Experience scale (DES). Two types of analyses were conducted: a person-centered (latent profile) analysis and various variable-centered tests to confirm the factor structure of IPO and calculate group differences.

Results

The three-factor (CFI = 0.990, TLI = 0.990, RMSEA = 0.022, SRMR = 0.089) and the five-factor (CFI = 0.995, TLI = 0.995, RMSEA = 0.014, SRMR = 0.090) models of the IPO was supported. Latent class analysis identified three subgroups of PO: “Well-integrated,” “Moderately integrated,” and “Disintegrated” classes. There were no significant differences between the three classes in the number of Axis 1 diagnoses (p = 0.354; η2 = 0.01). Group differences in the number of PDs, the number of PD symptoms as well as in the presence of borderline and depressive PD were significant (all p < 0.001; V = 0.35–0.42; η2 = 0.15–0.26). Persons with more severe PO problem level had higher rates of psychopathological symptoms, state and trait anger, and dissociative characteristics (all p < 0.001; η2 = 0.13–0.36).

Conclusion

The IPO can be an appropriate instrument to measure the severity of personality disorganization and to classify participants along a continuum of severity in this regard. Our results present further evidence that the severity of personality dysfunction, the central dimension of the ICD-11 and the Alternative Model for PDs is detectable with an instrument, the IPO, that was initially developed to detect the disturbances in PO.