bluuebunny/crossref_metadata_2025 huggingface dataset:
Influence of Political Guidelines and National Health Strategies on Resource Mobilisation for Integrated Community Care in Burkina Faso
Authors: Ouedraogo Hamed Sidwaya , Kabore Ahmed , Traore Badra Ali , Mano Moumai , Drabo Maxime Koine
Published: December 2024

Background: High infant mortality and inadequate access to care have led Burkina Faso to offer integrated community care for childhood illnesses (iCCM) for many years. Studies show that uptake is low and infant and child mortality remains high. The aim of the study is to analyse the influence of policies and strategies on the implementation of community-based management of childhood illnesses. Methods: A mixed methods cross sectional study was conducted in Burkina Faso in the health districts of Boussouma and Boussé. Data was collected from February to March 2023 using interviews of 29 people and administered questionnaires to 42 health workers. Descriptive statistics and thematic analysis was used respectively for quantitative and qualitative analysis. Nvivo 14 was used for qualitative analysis.Results: Overall, 29 people from various structures and 42 health workers took part in this study. The iCCM is well described and integrated into strategic documents and several activities are planned by stakeholders. Despite this strategic commitment, geographical coverage is still insufficient, according to our respondents. Financial resources and especially health products are not enough to meet needs. Conclusions: There is effective ownership of policy orientations and guidelines, but only partial. Vigorous action is needed on governance and funding alignment. The study identified the level of institutionalisation of iCCM and the efforts needed to improve coverage, particularly in terms of funding for drugs and equipment by government.
MAKING A SIMULATED AGREEMENT DEED BY A NOTARY AS THE CAUSE OF A LAND DISPUTE IN GIANYAR BALI (Case Study of Decision Number 259/Pdt.G/2020/PN. Gin)
Authors: Tama Evan Mandala
Published: December 2024

This study aims to analyze the making of simulated agreement deeds by notaries as the cause of land disputes in Gianyar, Bali. The main focus of this study is to examine the legal impact and potential conflicts that may arise due to the practice of making simulated agreement deeds. This research uses a normative juridical method, which is based on legal research through literature review. The results of the study show that the creation of a simulated agreement deed by a notary has the potential to cause land disputes, especially if it does not meet the applicable legal provisions. Factors such as the validity of the agreement, lack of clarity of information, and potential abuse of position can be a source of dissatisfaction and conflict among the parties involved. This research provides in-depth insight into the complexity of legal and social problems that can arise from the practice of making simulated agreement deeds. The implications of these findings show the need to improve the regulatory system and notary practices to prevent land disputes and increase public trust in the process of making deeds by notaries. The results of the study also stated that the deed of simulated agreement between David John Lock and Anak Agung Gede Oka Yuliartha was declared contrary to the law, so it was invalid, null and void, and did not have binding legal force. As a result of this agreement, the object of sale and purchase in the form of land covering an area of 200M2 and Certificate of Ownership Number: 2725/Desa Mas covering an area of 1,150 M2 became a dispute. The study also highlights the importance of a better understanding among notaries of the legal implications of creating simulated deed of agreement, as well as the need for stricter oversight to ensure that each deed made complies with applicable legal standards. Thus, this research is expected to make an important contribution to the development of notary law and the enforcement of justice in the settlement of land disputes.Keywords: Notary, Gianyar Bali, Preparation of Simulated Agreement Deed
HUBUNGAN ASUPAN MAKAN, PERSEPSI CITRA TUBUH, DAN PERILAKU PENGENDALIAN BERAT BADAN DENGAN STATUS GIZI GURU DI PERGURUAN MUHAMMADIYAH RAWAMANGUN
Authors: Azizah Fina Nailatul , Rahayu Leni Sri
Published: December 2024

Overnutrition is one of the most common nutritional problems in the adult age group. Overnutrition among working adults can harm productivity at work. This is because excess nutritional status can cause various non-communicable diseases such as type 2 diabetes mellitus, coronary heart disease, and cancer.  The purpose of this study was to determine the relationship between food intake, body image perception, and weight control behavior with nutritional status at the Muhammadiyah College of Rawamangun. The research was conducted in August 2023, involving 61 teachers. The research method used is quantitative research with a cross-sectional design and total sampling method for sample selection. Data was analyzed using the Chi-Square statistical test. Dietary intake was measured using a 2x24-hour recall method, body image perception and weight control behavior were assessed using Body Shape Questionnaire (BSQ) Short Version also waist circumference, body weight and height were measured using anthropometric measurements. The results of data analysis indicate a significant relationship between energy intake, protein intake, fat intake, carbohydrate intake, body image perception, and weight control behavior with nutritional status based on BMI and waist circumference (P<0.05). However, no significant relationship was found between body image perception and weight control behavior with nutritional status based on waist circumference (p>0.05). In conclusion, there were significant relationship between energy intake, protein intake, fat intake, carbohydrate intake, body image perception, and weight control behavior with nutritional status based on BMI and waist circumference.
Inter Dialytic Weight Gain and Ultrafiltration Goal of Post Covid-19 among Hemodialysis Patients in Riau Province: A Descriptive Study
Authors: Bayhakki Bayhakki , Huda Nurul , Aziz Ari Rahmat , Devyna Yulia , Lai Claudia. K. Y.
Published: December 2024

Key indicators for assessing the adequacy of hemodialysis include Inter-Dialytic Weight Gain (IDWG) and Ultrafiltration Goal (UFG), both of which are closely related to patients' hemodynamic status. However, there is limited information on the specifics of IDWG and UFG, particularly in Riau Province. This quantitative study with a descriptive design aims to describe IDWG and UFG in the aftermath of the COVID-19 pandemic in Riau. The research was conducted across three regional hospitals in Riau Province, involving 138 hemodialysis patients. This study employed univariate statistical analysis, using secondary data as the primary source. The findings indicate that in the first four months following the COVID-19 pandemic among hemodialysis patients in Riau Province, the average UFG and IDWG levels exhibited fluctuations, with IDWG in the three hospitals ranging from 2.28 to 3.12, while UFG was be 2.13 to 3.09. The results suggest that stabilizing of IDWG and UFG is crucial to prevent complications arising from these fluctuations, such as activation of the renin-angiotensin-aldosterone system, stimulation of the sympathetic nervous system, and increased cardiac output.
Determinants of Nutritional Status among Malnourished Children in Agricultural Areas
Authors: Susanto Tantut , Herminiati Ainia , Rohmawati Ninna , Yunanto Rismawan Adi , Rahmawati Ira , Bachtiar Syahroni , Merina Nuning Dwi , Zolkefli Yusrita Binti
Published: December 2024

Stunting in children is a major global issue, especially in rural agricultural areas of Indonesia. Despite ongoing efforts to eliminate stunting, its prevalence remains high, adversely affecting children's cognitive and physical development. This study aimed to determine the factors influencing the nutritional status of stunted children in Indonesia’s agricultural districts. A cross-sectional analytical study was conducted in Jember District, Indonesia, involving 236 malnourished children aged 0 to 59 months. Using stratified random sampling, structured questionnaires were utilized to collect data on family functioning, child feeding attitudes, and practices. The results are Family Function (B=0.038; 95% CI= 0,535 – 1,658, p value= 0.047); Child Feeding Attitude (B=0.030; 95% CI= 0,882 – 1,681, p value= 0.023); and Child Feeding Practice (B=0.120; 95% CI= 0,686 – 1,971, p value= 0.000). There were significant correlations between family function (p value= 0.047), child-feeding attitudes (p value= 0.023), and child-feeding practice (p value= 0.000) with children's nutritional status. This study highlighted a significant relationship among family function, child-feeding attitude, and practices concerning the nutritional status of children experiencing stunting. It emphasizes the importance of improving family function and supporting healthy child-feeding practices. Targeted interventions based on local cultural understanding are critical for effectively reducing stunting.
Activated Charcoal Magnetic Composite from Jengkol Peels as An Efficient Adsorbent for Aquatic Antibiotic Removal: An in Vitro Study
Authors: Wardani Gatut Ari , Nurhidah Mia , Wulandari Winda Trisna , Nofiyanti Estin , Syahputra Ricky Andi , Indra Indra
Published: December 2024

Antibiotic resistance has become a global issue that is quite worrying because it can threaten the survival of living things, especially humans. This resistance can occur due to the inappropriate use of antibiotics by the community. This study aims to determine the ability of activated charcoal magnetic composites (ACMC) to adsorb tetracycline hydrochloride compounds. Samples were obtained from jengkol peel waste which was charred and activated using acid and the addition of magnetic properties. The more PbFe2O4 added to activated charcoal, the greater the magnetic properties, surface area, pore-volume, and pore radius. The results showed that a type of IV adsorption isotherm which is a characteristic of mesoporous materials with a pore size of 2.0892 nm. The addition of magnetic properties to activated charcoal increase 29.33% the amount of tetracycline that is adsorbed by the adsorbent with optimal absorption in alkaline conditions. The adsorption process followed the adsorption kinetics of Ho and the Freundlich adsorption isotherm with an adsorption capacity of 76.3359 mg/g. ACMC material can potentially be one of the adsorbents that can reduce contamination of tetracycline hydrochloride antibiotics in the aquatic environment so that antimicrobial resistance can be minimized.
Keyword: acidity, agricultural waste, carbonization, functional Group, VSM analysis
Scarlet Honeyeater Myzomela sanguinolenta: An example of opportunistic irruption and nomadism heralding climate-facilitated range expansion?
Authors: Palmer Grant
Published: December 2024

Shifts in the traditional range of bird species are being reported globally, and often are associated with changing
climate. Much conservation focus is on species that have undergone range contractions, but range expansions also can have important implications for future scenarios. Using comprehensive databases of species records, many generated through citizen science, shifts in the spatial and temporal distribution of an Australian honeyeater, the Scarlet Honeyeater Myzomela sanguinolenta, are investigated at the southern boundary of its range in south-eastern Australia. Historically, this boundary of the species’ range was restricted to far eastern Victoria, with sporadic irruptions and vagrant records recorded infrequently further west. This study shows a recent significant increase in the spatial and temporal distribution of this species beyond its traditional range, expanding into central and western Victoria. Trends in records through time in the expansion zone display a clear shift, from vagrant and irruptive movements, to now show a pattern of regular, migratory movement behaviour. It was determined that within the expansion zone the Scarlet Honeyeater is now best considered a partial migrant. This range shift shares similarities with broader patterns of species’ range shifts in response to changing climate, warranting further investigation of the climatic niche of this and other species to understand potential changes in distribution into the future.
EKSEKUSI CASH COLLATERAL DALAM HAL DEBITUR WANPRESTASI (Studi Kasus di Bank BPR Intidana Sukses Makmur)
Authors: Surya Agustinus Aditya , . Pranoto
Published: December 2024

Artikel ini mengkaji dan mendeskripsikan tentang bagaimana pengikatan cash collateral sebagai jaminan kredit serta bagaimana eksekusi cash collateral apabila debitur wanprestasi di Bank BPR Intidana Sukses Makmur. Penelitian ini menggunakan metode penelitian hukum empiris dengan sifat penelitian deskriptif. Pendekatan dalam penelitian ini yaitu pendekatan kualitatif. Jenis dan sumber data yang digunakan berupa data primer yaitu hasil wawancara serta data sekunder yang berupa bahan hukum primer yaitu peraturan perundang-undangan dan bahan hukum sekunder berupa buku, jurnal, artikel, karya ilmiah, dan hasil penelitian yang relevan. Hasil penelitian menunjukkan bahwa pelaksanaan eksekusi cash collateral apabila debitur wanprestasi, Bank BPR Intidana Sukses Makmur dapat melakukan eksekusi secara langsung (parate eksekusi) yang didasarkan dengan adanya surat kuasa pencairan yang telah dibuat bersamaan dengan perjanjian kredit.
PENAMBAHAN PERYSARATAN PADA TENDER PEKERJAAN KONSTRUKSI DITINJAU DARI HUKUM PERSAINGAN USAHA
Authors: Permata Bintang Puwan
Published: December 2024

Procurement of goods and services should be carried out with the spirit of creating fair competition and should involve as many business entities as possible with equivalent classifications and types of businesses. Additional requirements in construction job tenders are typically motivated by the client's desire to secure credible and quality suppliers of goods/services. However, these reasons are often exploited by procurement actors to favor suppliers with specific relationships, thereby undermining fair competition. This article aims to examine provisions related to additional requirements in construction job tenders. It is a normative legal research using legislative and theoretical approaches. Secondary data in the form of legal materials will be analyzed using deductive reasoning. The conclusion drawn from this article is that adding requirements such as the provision of material supplier endorsements in construction tenders violates principles of fair competition if such endorsements steer towards only one specific factory/distributor
Analisis Bahaya dan Risiko Banjir Berbasis Multikriteria di Kecamatan Sirimau Kota Ambon
Authors: Lasaiba Mohammad Amin , Leuwol Ferdinand S
Published: December 2024

This study targets the threat and risk of flooding in Sirimau District, Ambon City, from a multi-criteria perspective using a Geographic Information System (GIS). Important flood hazard characteristics include land use, elevation, slope, distance from rivers, soil, and rainfall. Two risk factors, namely population density, land use, and flood hazard characteristics are used in flood risk analysis. Map aggregation procedure for flood risk and hazard analysis uses the Weighted Linear Combination (WLC) approach. The results of the flood hazard at the study site revealed that the flood hazard category was very high and high. Namely, 12.26% and the flood hazard category was very low and low, 87.84% and only. The results of the flood risk in the research location revealed that the flood risk with very high and high-risk categories was around 17.28%, and deficient and low flood risk categories (82.77%). This is because hilly and mountainous areas mostly dominate the Sirimau sub-district
indonesia ANALISIS NILAI KUAT TEKAN BETON AKIBAT SUBTITUSI METAKAOLIN DENGAN VARIASI METODE PERLAKUAN PERAWATAN BETON
Authors: Nadyashafiras  , Hermawan Okky Hendra , Utami Miranti Yulinaningtyas
Published: December 2024

Cuaca panas yang melanda Indonesia telah menjadi perhatian utama masyarakat. Penelitian ini bertujuan untuk membandingkan nilai kuat tekan beton pada umur 14 dan 28 hari, dengan variasi substitusi metakaolin pada semen (0%, 5%, 10%) dan metode perawatan yang berbeda. Metode eksperimen dan analisis statistik SPSS'22 digunakan dalam penelitian untuk mengevaluasi pengaruh perawatan pada suhu kamar dan di lapangan terbuka terhadap beton. Penelitian ini membandingkan metode perawatan perawatan beton pada suhu kamar dan di lapangan terbuka, dengan variasi substitusi metakaolin (0%, 5%, 10%) pada beton dengan mutu F'c 16 MPa. Hasil kuat tekan beton pada umur 14 hari: 16,5 MPa, 16,7 MPa, 16,4 MPa, 16,7 MPa, 15,7 MPa, 15,5 MPa. Hasil kekuatan tekan beton pada umur 28 hari adalah: 16,5 MPa, 16,9 MPa, 15,9 MPa, 17,8 MPa, 17,4 MPa, 16,5 MPa. Pengaruh statistik menggunakan SPSS dengan pengujian regresi linier berganda menunjukkan signifikansi pengaruh variabel X terhadap variabel Y pada sampel beton umur 28 hari.
Kata kunci: Kuat Tekan Beton, Metakaolin, Perawatan Beton,
Exploring the role of interactive games in improving Spanish spelling skills: A Mobile App study
Authors: Vázquez-Cano Esteban , Bernal-Bravo César , Ballesteros-Regaña Cristóbal , López-Meneses Eloy
Published: December 2024

Este estudio tiene como objetivo investigar el impacto de una aplicación móvil especialmente diseñada, GAUBI, en la competencia ortográfica del español por estudiantes de Educación Primaria. Utilizando un diseño cuasi-experimental, se plantea la hipótesis de que GAUBI mejoraría significativamente el rendimiento ortográfico. Además, examinamos la relación entre el tipo y la duración del juego, específicamente los juegos arcade, de investigación y de ficción, y las mejoras en la competencia ortográfica. El estudio involucró a 114 estudiantes españoles de Educacion Primaria, quienes completaron un pretest para establecer su nivel de competencia ortográfica. Tras un período de uso de la aplicación, se administró una prueba posttest para evaluar los cambios en el rendimiento. El contenido de las pruebas pretest y posttest se alinearon con el currículo de Educación Primaria en español. Las pruebas t de muestras emparejadas compararon las puntuaciones de los resultados del pretest y posttest, revelando mejoras estadísticamente significativas en el rendimiento ortográfico, con un tamaño del efecto grande (d de Cohen = 0.80). Los coeficientes de correlación de Pearson exploraron la asociación entre el tipo de juego y los resultados ortográficos, identificando correlaciones positivas significativas para los juegos de "Investigación" y "Ficción", mientras que los juegos "Arcade" no mostraron una relación clara. Estos hallazgos sugieren que los juegos basados en narrativas e investigación son particularmente efectivos para mejorar las habilidades ortográficas. Los resultados apoyan el uso de la gamificación, especialmente a través de juegos colaborativos y ricos en narrativas, como una estrategia beneficiosa para practicar la ortografía en español.
Development of a high-throughput assay for monitoring adipogenesis in vitro
Authors: Giles Rachel , Durandt Chrisna , Ambele Melvin , Pepper Michael
Published: December 2024

The obesity pandemic is listed as a chronic disease by the World Health Organization, and is underpinned by an increase in fat cell formation (adipogenesis). This study aims to develop high-throughput screening assays (flow cytometry, spectrophotometry and RT-qPCR) for cost-effective and rapid monitoring of adipogenesis using a human adipose-derived stem cell differentiation model. Adipogenesis was successfully upscaled from 6- to 96-well plates. We found that the most efficient well size differs between the assays investigated. Adipogenic differentiation could best be assessed in a 48-well plate (flow cytometry and spectrophotometry), while a 12-well plate was more efficient for obtaining reliable RT-qPCR results. The cost associated with hypothetically screening 100 compounds (in a high-throughput setting) using the method with the lowest degree of variability and the highest degree of cost-effectiveness was spectrophotometry (48-well plate), followed by flow cytometry (48-well plate) and RT-qPCR (12-well plate). This study provides valuable information for designing and selecting the most efficient high-throughput adipogenesis assays to screen for potential drug candidates in the continuous search of novel and more effective pharmacological agents to combatting obesity and its related comorbidities.
Global, Regional, and National Burden of Benign Prostatic Hyperplasia: A Comprehensive Analysis from 1990 to 2021
Authors: Chen Xin , Yang Siyuan , He Zhangxin , Chen Zihao , Tang Xinling , Lin Yuxin , Huang Yuhua , Hou Jianquan , Wei Xuedong
Published: December 2024

Benign prostatic hyperplasia (BPH) is a common urological condition affecting elderly men worldwide. In 2021, there were 112,502 (95% UI: 88,131.8–142,634.2) thousand prevalent cases globally, compared to 50,705.8 (95% UI: 38,735.5–65,693.4) thousand cases in 1990, representing a 122% increase. Over the past 30 years, the burden of BPH in low and low-middle socio-demographic index (SDI) regions have shown an upward trend and is projected to continue increasing over the next 15 years. Middle-SDI regions are facing the heaviest absolute burden of BPH. Despite declining prevalence, incidence, and disability-adjusted life years rates in high-middle SDI regions, the absolute BPH burden remains high, ranking second among the five SDI regions. In contrast, high-SDI regions exhibit a relatively low and stable BPH burden, though significant variations exist even among countries within the high-SDI category. Additionally, the global 65–69 age group bears the highest burden, with the 40–44 and 80 + age groups showing increasing trends. The burden of BPH varies significantly across regions, socioeconomic statuses, and countries, yet the absolute burden is generally increasing. The substantial regional differences in BPH burden underscore its widespread impact and potential controllability, indicating the need for more targeted healthcare efforts to address the growing BPH burden.
Ethnobotanical Survey of Cerrado Plants Used as Antihypertensives for the Elderly in Rondonópolis, MT, Brazil.
Authors: Santos Gleyciany Stefany Alves , Piza Paulo Adaias , Toniasso Isabela Monteiro , Lacerda Maryland Sanchez , Melo Laura Valdiane Luz , Silva Marcondes Alves Barbosa da
Published: December 2024

Ethnobotany studies the aspects by plant utilization by societies, encompassing principally the medicinal utility. Consequently, it helps in the development bioactive natural products. This ethnobotanical study was focused medicinal plants to the Cerrado, as used by elderly in Rondonópolis-MT, Brazil for hypertension treatment. Initially, two institutions supporting elderly in Rondonópolis were chosen: Recanto do Idosos and the Associação de Apoio à Terceira Idade. A questionnaire, comprising open and closed questions covering personal data, general plant-use information, and knowledge about species with anti-hypertensive properties, was used. Plants cited as anti-hypertensive and originating from the Cerrado were collected and submitted for taxonomic classification. A total of 41 elderly participated in the study, with 60.08% women, and 39.02% having incomplete elementary education. Notably, mothers played a prominent role, accounting for approximately 38.71% of the influence on plant use. Among the respondents, 58.06% believed that plants exhibit a moderate effect, while 100% claimed that plants do not cause adverse effects, and 93.5% affirmed the transmission of this knowledge to younger generations. In conclusion, 11 medicinal plants with anti-hypertensive activity were identified, with only 4 classified as belonging to the Cerrado post-bibliographic research. Following collection and botanical classification, the scientific names obtained were: Costus arabicus L.; Solanum paniculatum L.; Acrocomia aculeata (Jacq.) Lodd. Ex Mart.; and Bixa orellana L. The limited number of plants mentioned raises concerns about the potential loss of traditional knowledge about plant among elderly. Hence, undertaking new ethnobotanical investigations becomes crucial for documenting this culturally significant knowledge.
A Girl with Immunoglobulin G4-Related Kidney Disease; A case report
Authors: Venugopal Sreeram , Gopalakrishnan Natarajan , Thanigachalam Dinesh Kumar , Abraham Anila
Published: December 2024

Introduction
: Immunoglobulin G4-Related Disease (IgG4-RD) is a rare condition, particularly in the pediatric population, and it is an uncommon cause of Acute Tubulointerstitial Nephritis (ATIN).
Case Presentation:
A renal biopsy of a girl with sudden onset renal failure revealed ATIN in the absence of any identifiable infections, medications, or obvious autoimmune disorders. Immunohistochemical staining of the renal biopsy showed IgG-positive plasma cells within the interstitium, along with elevated plasma IgG4 levels. These findings led to a diagnosis of immunoglobulin IgG4-RD of the kidney, a rare finding in this age group. The girl responded well to a combination therapy of steroids and Mycophenolate Mofetil (MMF).
Conclusion
IgG4-RD should be considered in the differential diagnosis of ATIN, especially with interstitial plasma cell infiltration and fibrotic changes in renal biopsy.
Prevalence of Vertebral Fractures in Patients with Acromegaly: A Multicenter Cross-Sectional Study
Authors: Almalki Mussa H. , Almohaya Mohammed , Aziz Faisal , AlDahmani Khaled M. , Alashgar Lolwah , Ekhzaimy Aishah , Mahzari Moeber
Published: December 2024

Introduction: Acromegaly is a chronic disease characterized by excessive growth hormone (GH) secretion, leading to high insulin-like growth factor 1 (IGF-1) levels. It results in systemic complications, including vertebral fractures (VFs), which impair quality of life. Bone mineral density (BMD) may not fully capture fracture risk, warranting further research into alternative predictors and mitigation strategies.
Objectives: This study aimed to assess the prevalence of VFs in patients with acromegaly and identify associated risk factors.
Methods: A multicenter, cross-sectional study was conducted at three centers in Saudi Arabia and one in the UAE. Data for patients with acromegaly evaluated between 2010 and 2024 were reviewed.
Results: The cohort consisted of 101 patients (63% male), with a median age of 45 years and an average disease duration of 7 years. VFs were present in 9.9% of patients, predominantly affecting the lumbar spine and were generally mild. Although not statistically significant, patients with VFs had longer disease durations and higher IGF-1 levels. BMD measurements did not significantly differ between those with and without VFs; however, patients with VFs showed a trend toward lower trabecular bone score (TBS), suggesting compromised bone microarchitecture.
Conclusion: This study underscores the importance of comprehensive bone health assessment in patients with acromegaly, incorporating BMD, TBS, and hormonal evaluations. Continuous long-term monitoring of bone health parameters is necessary in this population. Future research is needed to delineate risk factors associated with VFs.
Evaluation of Panoramic Radiographic Measurements in Subadults Aged According to Age and Sex
Authors: Okkesim Alime , Erhamza Turkan Sezen
Published: December 2024

Background
The study utilized panoramic radiography measurements to evaluate age and sex in individuals aged 9–17 within the Turkish population. The study aimed to address the gaps in the literature by focusing on adolescence and pre-adolescence and investigating the impact of puberty on mandibular parameters in both sexes. Through this inquiry, this study sought to contribute to the refinement of forensic anthropological methodologies, particularly in cases involving subadult mandibular remains of unknown age or sex.
Methods
The sample comprised 631 patients, including 326 females and 305 males. Panoramic radiographs were obtained using a specific model with standardized exposure parameters. Participants were 9–17 years of age. Various mandibular parameters were measured, including the bigonial width, condylar height, ramus breadth, coronoid height, projective ramus height, mandibular cortical index, gonial angle, antegonial angle, and antegonial height. The data were analyzed using descriptive statistics, the Mann-Whitney U test, and Univariate Linear Regression Analysis.
Results
There were significant differences in several mandibular parameters between males and females across different age groups. For instance, females generally exhibited higher values in condylar height, coronoid height, projective height of ramus, mental index, and mandibular cortical index, while males showed higher values in min ramus breadth, antegonial measurements, and bigonial width. However, the gonial angle did not show significant differences between sexes. The regression analysis showed that condylar height and coronoid height were significant predictors of age in both males and females.
Conclusions
Panoramic radiography measurements are reliable tools for age and sex determination in subadult individuals within the Turkish population. The findings underscore the importance of mandibular parameters in forensic anthropology and suggest the potential of panoramic radiography in aiding forensic investigations and identification processes. Further large-scale and multicenter studies are recommended to validate the applicability and accuracy of these parameters across diverse populations.
Dissecting the Role of Cellular Senescence in Meningioma Recurrence: Integrative Bioinformatics and Elastic Network Modeling
Authors: Huang Jian-huang , Chen Yao , Kang Yuan-bao , Lin Cai-hou
Published: December 2024

Background
Cellular senescence is intimately tied to tumorigenesis and progression, yet its exploration in meningiomas remains inadequate. In this study, we aim to unravel the role of cellular senescence-associated genes (CSA-genes) in meningioma recurrence and identify potential diagnostic markers and therapeutic targets.
Methods
We analyzed GSE136661 and GSE173825 datasets to identify CSA-signature genes through differential expression analysis, weighted gene co-expression network analysis, protein-protein interaction network construction, and elastic net regression modeling. Functional enrichment, immune cell infiltration using CIBERSORT, and transcription factor prediction were performed. Potential drugs were screened using Enrichr database.
Results
CDK1, FOXM1, MYBL2, and BIRC5 emerged as key CSA-genes related to cell cycle and DNA damage. Recurrent meningiomas showed immune heterogeneity, with CSA-genes correlating with immune infiltration and checkpoint molecules. E2F1 was predicted as a regulator. Dasatinib and Rapamycin showed promising anti-meningioma potential.
Conclusion
Our findings highlight crucial genes and pathways in meningioma recurrence, introducing novel therapeutic candidates. These findings pave new avenues for further elucidating meningioma recurrence mechanisms and developing innovative treatments.
A risk prediction model for nausea and vomiting after TACE : a cross-sectional study
Authors: Dai Yali , Zeng Mengting , He Hong , Cai Miao
Published: December 2024

Purpose
It was found that 52.5% of patients experienced nausea and vomiting after Transcatheter Arterial Chemoembolization (TACE), but there are no models that predict this risk. In this study, we investigated the factors associated with nausea and vomiting after TACE and developed a predictive model to predict these adverse events.
Method
The study will include 401 patients who will be randomly assigned to the training group and validation group in a 7:3 ratio. An analysis of logistic regression was used to identify predictors and build a risk prediction model. Model performance was evaluated using the Area Under Curve (AUC), Calibration Curve, and Decision Curve Analysis (DCA).
Results
This study ultimately included 401 patients for TACE, of whom 132(32.92%) patients experienced nausea and vomiting. There were independent risk prediction models for the history of vomiting, the preventive use of antiemetic, pain, and platinum and anthracene. Testing and training groups had AUCs of 0.839 and 0.742, respectively. They had calibration curves with P = 0.208 and P = 0.482, respectively. The DCA curves showed that the model had good clinical benefit at threshold probabilities greater than 20%.
Conclusion
The prediction model has good discriminatory ability, accuracy evaluation and clinical efficacy and is presented by nomogram with strong operability, contributing to for clinical medical staff to improve the management of TACE.
Recombinant expression of a new antimicrobial peptide composed of hBD-3 and hBD-4 in Escherichia coli and investigation of its activity against multidrug-resistant bacteria
Authors: Yan Han , Ning Nianzhi , Yu Wenjing , Zhang Liangyan , Cao Binwang , Li Deyu , Zhang Xingxiao , Wang Hui
Published: December 2024

Human β-defensin(HBD), a short cationic peptide found in humans, exhibits broad-spectrum antimicrobial activity, making it a significant candidate for antimicrobial drug development. In previous studies, we fused human β-defensin 3 and human β-defensin 4 to create a chimeric human β-defensin, named H4, with enhanced antimicrobial activity and improved salt stability. However, due to the relatively large number of amino acids in H4, the cost of its chemical synthesis is significant. The objective of this study is to explore a method for producing H4 using a prokaryotic expression system to reduce its manufacturing costs. We first optimized the codons of H4 to make it suitable for expression in prokaryotic systems. Subsequently, the optimized Open Reading Frame of H4 was cloned into the pET32a vector and combined with thioredoxin and enterokinase cleavage sites to reduce its toxicity to prokaryotic cells. The resulting plasmid was introduced into E. coli BL21, and then the expression was induced using IPTG to produce a fusion protein (TrxA-EK-H4). TrxA-EK-H4 was isolated using a nickel column, and then EK cleavage was performed to generate unlabeled H4. Our results indicated that TrxA-EK-H4 can be cleaved by EK when using a nickel column, but it does not undergo precise cleavage in Tris-HCl buffer. The antimicrobial assays showed that the recombinant H4 exhibited similar bactericidal activity against multidrug-resistant Acinetobacter baumannii as the chemically synthesized H4. In summary, this study has developed a prokaryotic expression system for H4. Our approach offers a novel experimental concept for the future purification of similar proteins that are difficult to cleave effectively.
Cerebrospinal fluid biomarkers of efficacy in patients affected by Spinal Muscular Atrophy type 1 treated with nusinersen.
Authors: Sframeli Maria , Polito Francesca , Vita Gian Luca , Macaione Vincenzo , Gitto Eloisa , Vita Giuseppe , Aguennouz M’hammed , Messina Sonia
Published: December 2024

Background/aim.
The advent of new therapies, such as the antisense oligonucleotide nusinersen, has significantly improved the natural course of spinal muscular atrophy (SMA). Tau proteins and neurofilaments are well known markers of axonal degeneration. The neurofilament light protein (NfL) has been proposed as a possible biomarker in SMA. This study aimed to investigate the role of total-tau (ttau), phosphorylated tau (ptau), NfL, and phosphorylated neurofilament heavy chain (pNfH) proteins as potential cerebrospinal fluid (CSF) biomarkers of axonal degeneration and response to nusinersen treatment in 14 SMA type 1 patients with a wide age range (2-156 months).
Methods and results
Motor functions was assessed using the “Children's Hospital of Philadelphia Infant Test of Neuromuscular Disorders” (CHOP-INTEND) scale at baseline, six months, and ten months after treatment. Eight out 14 patients showed motor improvement. At baseline CSF ttau and ptau concentration showed a significant negative correlation with age (p = 0.0002 and p = 0.0054 respectively) and a positive correlation with the CHOP-INTEND score (p = 0.0075 and p = 0.0342, respectively). After treatment the tau biomarkers did not show any change, whereas NfL and pNfH concentration significantly decreased (p = 0.0001). The NfL concentration decline related to age at baseline (p < 0.05). There was also a significant correlation between the decrease of NfL and the improvement of the CHOP-INTEND motor score, but only in the subgroup of patients with a functional improvement above 3 points (p < 0.05).
Conclusions
CSF NfL may be a powerful biomarker for monitoring treatment response to nusinersen both in younger and older patients with severe SMA.
Pathological Features and Immunohistochemical Characteristics of Clear Cell (Glycogen-Rich) Urothelial Carcinoma: A Case Report and Systematic Review of the Literature
Authors: wang weiming , Zhang Baogang , Wu Zhangli , Hou Chuanling , li mengyao , Zhang tianyi
Published: December 2024

Clear cell (glycogen-rich) urothelial carcinoma is an exceedingly rare variant of invasive urothelial carcinoma, distinguished by the presence of abundant cytoplasmic glycogen, which imparts a clear appearance to the tumor cells under histological examination. In this case report, the diagnosis was established through histopathological evaluation with hematoxylin and eosin (HE) staining, immunohistochemical analysis, and the identification of significant cytoplasmic glycogen accumulation. The patient, an 89-year-old male, was admitted on July 24, 2024, presenting with painless gross hematuria persisting for one week. Abdominal ultrasound and CT urography revealed a soft tissue mass on the right side of the bladder wall, measuring 30×30 mm, with a broad base connected to the bladder wall. The mass exhibited significant enhancement on contrast-enhanced scans, raising suspicion for malignancy. Microscopic examination revealed two distinct tumor cell morphologies: the conventional urothelial carcinoma pattern and a clear nest-like morphology, with the latter comprising over 70% of the tumor. Immunohistochemical staining for GATA-3, CK7, PAS, PAS-D, CAIX,PAX8,and RCC confirmed the diagnosis of clear cell (glycogen-rich) urothelial carcinoma. This rare variant of invasive urothelial carcinoma underscores the need for detailed diagnostic analysis to inform prognosis and treatment strategies.
Impact of Vitamin D3 Supplementation on Motor Functionality and the Immune Response in Parkinson's Disease Patients with Vitamin D Deficiency
Authors: LI Danfeng , Ma Xibo , Zhang Wentao , Zhong Ping , Zhu Xiaowei , Chen Yan , Liu Shihua
Published: December 2024

The purpose of this study was to investigate the levels of vitamin D, regulatory T (Treg) cells, and T helper 17 (Th17) cells in the peripheral blood of patients with Parkinson's disease(PD), explore their potential relationships with PD, and further examine the effects of vitamin D intervention on these biomarkers and clinical outcomes. PD patients with low vitamin D3 levels were randomly assigned to two groups: those supplemented with vitamin D3 (VitD3, n=15) and those treated with vegetable oil (PL, n=15). Treatment was administered continuously for 3 months. Vitamin D3, Th17 and Treg levels and related clinical scales were continuously monitored and evaluated. The results revealed that the level of 25(OH) D3 in the peripheral blood increased in the VitD3 group, and the Th17 and Treg levels tended toward those of the normal population. Compared with those of the preintervention groups and the PL group after the 3-month follow-up, motor function improved in the VitD3 group. Additionally, vitamin D was negatively correlated with Th17 cells and positively correlated with Treg cells. In PD patients, motor function was positively correlated with vitamin D and Treg levels but negatively correlated with Th17 levels. We believe that it is necessary for PD patients with low vitamin D levels to supplement vitamin D to normal levels.
Interfacial Coupling effects of Chitosan integrated ZrO 2 /Bi 2 O 3 /CeO 2 quaternary composite for efficient wastewater treatment and antimicrobial activity
Authors: Nisha U. Mary , Venkatesh D. , Vasanthan S. , Rajeswaran P. , Balaji J. , Karthik P. Siva
Published: December 2024

The quaternary phase of chitosan incorporated (CeO2/ZrO2/Bi2O3) composites synthesized by a simple co-precipitation technique. XRD and XPS analysis can study the phase identification and chemical states. SEM analysis revealed that the composites exist as nanoflakes morphology with agglomerated nanoparticles. Thermogravimetric analysis observed the decomposition temperature of the composites ranges between 680–760°C. Bruner-Emmett-Teller analysis showed 27.77 m2/g surface area and 0.003cc/g pore volume of the composite. The quaternary phase of the composite showed 92% degradation efficiency for malachite green at 180 min time intervals and 93% degradation efficiency for Acid Blue 113 at 240 min time intervals under solar light illumination. The photodegradation of MG and AB 113 was carried out using an optimal amount of catalyst dosage (9 mg), pH (7 & 9), dye concentration (10 ppm), and electrolytes (Na2CO3, NaHCO3). The ESI mass analysis of each degraded product of the dye molecule was recorded, and the structural information was provided. The reusability test exposed the stability of composites for up to three cycles, and consistent results were obtained each cycle. The microbial studies were investigated for the composites, and results showed efficient anti-bacterial activity on Escherichia coli at 100µl/mL concentration. The anti-fungal studies revealed the efficient activity of Candida albicans and Aspergillus niger for the composites.
Photocatalytic removal of synthetic dyes using Bi2O3–TiO2 nanocomposites obtained by simple hydrothermal route
Authors: Ruiz-Santoyo Victor , García-Carvajal Sarahí , Arenas-Arrocena M. Concepción
Published: December 2024

The current study reports the preparation of TiO₂-modified Bi₂O₃ photocatalysts with different TiO₂ contents, synthesized via an in-situ hydrothermal method. The powder samples were characterized by XRD, SEM, TEM, FTIR–ATR, UV-vis, XPS, and N₂ physisorption analysis. The photocatalytic activity of the TiO2-modified Bi2O3 was studied for the removal of methyl orange (MO) and methylene blue (MB) under different reaction conditions. Our study showed that the TiO2-modified Bi2O3 photocatalysts exhibited superior photocatalytic activity compared to the pristine samples of TiO2 and Bi2O3. The BiT16 sample achieved degradation rates of approximately 93.9% and 98.2% for MO and MB, respectively, within 120 minutes of reaction at 30 ppm. These results are closely related to the band gap values, differences in textural features, TiO2 content, and the reduction in the recombination process of e⁻/h⁺ pairs in the Bi2O3–TiO2 composites. Reaction kinetics were determined using the Langmuir–Hinshelwood mechanism, and during the third photoreaction cycle, the TiO2-modified Bi2O3 (BiT16) achieved photocatalytic degradation rates of 65.6% for MO and 70.5% for MB.
Enhancing aquaculture sustainability and profitability: Effects of black soldier fly larval meal on the performance of African Catfish (Clarias gariepinus) in Cameroon
Authors: K. Hervé Mube , D. Calice Mekeu , Dzepe Daniel , F. Rousseau Djouaka , Chia Shaphan Y. , Efole Thomas , Ndindeng Sali A. , Tendonkeng Fernand
Published: December 2024

High cost and scarcity of feed ingredients have continued to challenge the aquaculture. This present study assessed the potential of black soldier fly (BSF, Hermetia illucens L.) larvae meal (BSFLM) as a sustainable alternative source of animal protein in the diet of African catfish (Clarias gariepinus), a species widely farmed in Cameroon for its breeding potential and economic. Five diet formulations: a control diet with 100% fishmeal (FM, BSFLM0), and diets where FM was substituted at 50% (BSFLM50), 75% (BSFLM75), and 100% (BSFLM100) levels, were compared. Larvae of BSF were processed using three different methods: toasting, sand toasting, and boiling. Parameters assessed included the chemical and microbial properties of BSF larvae, catfish growth performance, health, consumer acceptance and economic viability of substituting FM with BSFLM. Our results demonstrate that boiling larvae significantly increased the dry and organic matter contents but reduced crude protein (CP) and fat levels, whereas toasting increased CP. Mineral content varied across processing methods: sand toasting had the highest calcium, boiling the highest iron, and toasting the highest potassium, sodium, and phosphorus levels. Sand-toasted larvae had the highest Escherichia coli levels, Lactobacillus was present only in boiled larvae, and there were no Enterobacteria or Salmonellain toasted or sand-toasted samples. At higher levels (75-100%), BSFLM improved catfish growth rates, feed conversion ratio, and survival compared to the control. Replacing FM with BSFLM also reduced production costs, increased profitability, and enhanced sustainability, as indicated by a lower fish-in-fish-out ratio. Inclusion of BSFLM significantly enhances the sustainability potential of aquaculture practices by reducing reliance on wild-caught fish for feed. Overall, BSFLM is a suitable alternative protein source that can support economic viability and environmental sustainability of aquaculture, promoting resilient fish farming practices.
Glass consumption and production at the turn of first millennium CE: chemical analyses of late antique and medieval glass from Koper (Capodistria, SI)
Authors: Šmit Žiga , Milavec Tina
Published: December 2024

A series of glasses from the site Kapucinski vrt (garden of the Capuchin monastery) in Koper (Capodistria) in northern Adriatic was measured by a combined PIXE and PIGE method. Koper has been continuously populated since late Roman period till the present with a rich medieval history, so the measurements were expected to show the trends in glass production and consumption from Late Antiquity until the Middle Ages, notably the transition between the natron to plant ash glass and supply of the fresh glass. Among the set 22 glass finds, both natron and plant ash glass were identified. Natron glass was of the types Foy 2.1 (with a noticeable component of Roman Mn) and HIMTa, all with the signs of recycling, while four samples (about 20% of the whole set) were unrecycled: they involve 2 examples of Levantine glass, an example of probably Mesopotamian origin and a lamp of mixed glass between the natron and plant-ash glass; this may indicate a modest supply of fresh glass during the period. Plant ash glass may be attributed to the Early or High Middle Ages, exploiting purified alkalis of the Levantine coasts (known as alume catino in Venetian glassmaking), while the admixture of impurities in the siliceous sands suggest circulation and consumption of glass that was produced and traded in the eastern Mediterranean since the 10th centuries onwards.
The Self-synthesis Function on the Construction of Anxiety: A Qualitative Analysis Based on Grounded Theory
Authors: Huang Ruizhi , Shen Huiqing , Qiu Huilin , Zha Shuyi , Jiang Ke
Published: December 2024

This study utilized a qualitative research approach known as the grounded theory method to explore the relationship between self-synthesis function and anxiety. Nineteen participants underwent semi-structured interviews, and MAXQDA2022 software was employed for coding assistance. The results revealed 125 open codes encompassing 2139 reference points. Eight axial coding codes emerged through dichotomous comparisons in three dimensions. Additionally, three destructive categories—"core factor," "sub-core factor," and "peripheral factor"—and two protective categories—"object-support" and "subject-effort"—were formed by clustering the number of reference points. In conclusion, (1) core factors contributing to anxiety primarily originate from the subjective aspect, while anxiety reduction mainly stems from the objective aspect. (2) Temporally, cognitive model disparities resulting from subject-object transformation are linked to anxiety. (3) The self-synthesis function mitigates anxiety by reducing disparities. (4) Adaptive anxiety signifies effective compensation by the self-synthesis function, whereas pathological anxiety results from its failure to compensate effectively.