bluuebunny/crossref_metadata_2025 huggingface dataset:
Unravelling the Link Between Primary Sclerosing Cholangitis and Sepsis: A Mendelian Randomization Study
Authors: Chen Ze-jun , Wu Fan-ye , Li Zheng-ran , Zhang Zi-ran , Sun Yu-xin , Wang Zi-jin , Meng Fan-ke , Xia Xun
Published: January 2024

Background: autoimmune diseases (ADS) are associated with sepsis. This
study aims to investigate the causalities between ADs and sepsis using
Mendelian randomization (MR). Methods: we extracted single-nucleotide
polymorphisms (SNPs) closely associated with 10 ADs, including systemic
lupus erythematosus (SLE), rheumatoid arthritis (RA), type 1 diabetes
(T1D), multiple sclerosis (MS), inflammatory bowel disease (IBD), celiac
disease (CD), psoriasis (PsO), primary sclerosing cholangitis (PSC),
primary biliary cholangitis (PBC), and ulcerative colitis (UC) from the
GWAS. Two-sample MR analysis was conducted using GWAS data from the UK
Biobank to assess the association between the liability of each ad and
sepsis and sepsis-related 28-day mortality. The inverse
variance-weighted (IVW) method was used as the primary analysis. If
sig-nificant causal relationships are found (considering multiple
comparisons) in the univariate MR analysis, multivariate MR (MVMR)
analysis is performed to adjust for body mass index (BMI) and smoking. A
series of sensitivity analyses were conducted to validate the robustness
of the results. Results: after adjusting for multiple testing, MR
analysis revealed that PSC patients are responsible for increased
susceptibility to sepsis using the IVW method (OR:1.033,
95%CI:1.007-1.060, PFDR = 0.020). Further sensitivity analyses
validated the robustness of the above association. Even after adjusting
for BMI and smoking, the MVMR-IVW still displayed a positive
correlation（OR:1.043, 95%CI:1.022-1.064, P-value for IVW = 3.32E-05）
between PSC patients and susceptibility to sepsis. However, no
significant causal relationship was observed between SLE, RA, T1D, MS,
IBD, CD, PsO, PBC, and UC with susceptibility to sepsis or short-term
death risk. Conclusions: our MR analysis revealed a genetic
susceptibility of PSC to sepsis. However, no causal relationship was
observed between SLE, RA, T1D, MS, IBD, CD, PsO, PBC, and UC with
suscep-tibility to sepsis or short-term death risk. Keywords: autoimmune
diseases; sepsis; primary sclerosing cholangitis; Mendelian
randomization
Digital Democracy using Digital Platform in South Korea: Right to Petition and Election Campaign
Authors: Roh Myunghoon , Son Hyeungseob
Published: January 2024

This Article examines how it can be utilized and rationally utilized
using a digital platform. Korea is reviewing the National Assembly Act
and the Petition Act, and this research promotes political opinions and
legislative efficiency through digital platforms. Also, in the election
campaign, it is important the implementation of a new democracy process
using a digital platform and its effects. In particular, this researches
how are digital platforms, YouTube/political applications/metaverse,
used in the 2022 presidential election in Korea and what effect they
have had, how can a digital platform deal with the rational choice of
voters or the side effects using big data combined with AI. This paper
presents what role should digital platforms play in order to reflect
public will, expand voters’ political participation. Therefore, this
paper studied legal ways to provide political opinions and accept
policies through digital platform systems such as of ‘electronic
petitions’, Metaverse, AI.
Electrochemical dearomative spirocyclization of N-acyl sulfona-mides in a continuous-flow cell
Authors: Liu Ting , Shi Zhaojiang , Yuan Yaofeng , Lin Yuqi , Ye Keyin
Published: January 2024

The development of efficient and sustainable methods to obtain
spirocyclic compounds is of significance as these structures are widely
found in pharmaceuticals and agrochemicals. Herein, we disclose an
electrochemical dearomative spirocyclization of N-acyl sulfonamides in a
continuous-flow cell. The reaction is simple and efficient without
external catalysts or supporting electrolytes, in-cluding a
decagram-scale synthesis.
DEVELOPMENT AND VALIDATION OF A METACOGNITIVE ASSESSMENT TOOL FOR TURKISH PRESCHOOL CHILDREN: A TEST FOR 48-66 MONTHS-OLD
Authors: İmir Hatice Merve , Ekici K. Büşra Kaynak , Temel Z. Fulya
Published: January 2024

This study addresses the assessment of metacognitive monitoring in
Turkish preschool children, a critical aspect of their learning and
development. A specialized paired-association task was designed to
create an assessment tool targeting higher-order thinking skills in this
demographic. Out of 190 participants aged 48 to 66 months, gathered from
three preschools in Ankara, Turkey, the data analysis centered on 160
children, excluding those with missing or incomplete responses. The
Metacognitive Thinking Test’s Judgment Part (Jp) underwent factor
analysis, revealing a two-factor structure with 14 items demonstrating
memory-confidence alignment. Notably, children tended to overestimate
inaccurate recollections, aligning with the Dunning-Kruger effect.
Despite limitations, the study offers valuable insights, shedding light
on the intricate relationship between confidence and correctness in
metacognitive development among young learners. This research lays the
groundwork for future investigations in this domain.
A Validated Mathematical Model of FGFR3-Mediated Tumor Growth Reveals Pathways to Harness the Benefits of Combination Targeted Therapy and Immunotherapy in Bladder Cancer
Authors: Okuneye Kamaldeen , Bergman Daniel , Bloodworth Jeffrey , Pearson Alexander , Sweis Randy , Jackson Trachette
Published: January 2024

Bladder cancer is a common malignancy with over 80,000 estimated new
cases and nearly 18,000 deaths per year in the United States alone.
Therapeutic options for metastatic bladder cancer had not evolved much
for nearly four decades, until recently, when five immune checkpoint
inhibitors were approved by the FDA. Despite the activity of these drugs
in some patients, the objective response rate for each is less than
25%. At the same time, fibroblast growth factor receptors (FGFRs) have
been attractive drug targets for a variety of cancers, and in 2019 the
FDA approved the first therapy targeted against FGFR3 for bladder
cancer. Given the excitement around these new receptor tyrosine kinase
and immune checkpoint targeted strategies, and the challenges they each
may face on their own, emerging data suggest that combining these
treatment options could lead to improved therapeutic outcomes. In this
paper, we develop a mathematical model for FGFR3-mediated tumor growth
and use it to investigate the impact of the combined administration of a
small molecule inhibitor of FGFR3 and a monoclonal antibody against the
PD-1/PD-L1 immune checkpoint. The model is carefully calibrated and
validated with experimental data before survival benefits and dosing
schedules are explored. Predictions of the model suggest that FGFR3
mutation reduces the effectiveness of anti-PD-L1 therapy, that there are
regions of parameter space where each monotherapy can outperform the
other, and that pretreatment with anti-PD-L1 therapy always results in
greater tumor reduction even when anti-FGFR3 therapy is the more
effective monotherapy.
Wheat (Triticum aestivum L.) seed priming with calcium chloride enhances resistance to wheat aphid (Schizaphis graminum Rondani, Hemiptera: Aphididae)
Authors: wang Jun , Song Jia , Wu Xiao-bao , Deng Qian-qian , Zhu Zhong-yan , Ren Mingjian , Ye Mao , Zeng Ren-sen
Published: January 2024

Calcium (Ca) is an essential macronutrient for plant growth. Although it
has been demonstrated that exogenous Ca application could increase plant
resistance to abiotic stress, its potential to enhance tolerance to
biotic stress remains unclear. Here, we investigated the development
time, population size, feeding behavior of aphids feeding on plants
grown from CaCl- and water-pretreated seeds, and plant
defenses against aphids. CaCl seed-pretreatment
increased aphid development time and reduced aphid population size and
feeding efficiency. Additionally, CaCl pretreatment
increased the concentrations of Ca in wheat leaves.
Furthermore, TaCaM genes were upregulated in plants by
CaCl pretreatment, and callose synthase genes
(TaGSL2, TaGSL8, TaGSL10, TaGSL12,
TaGSL19, TaGSL22, and TaGSL23) were activated.
Callose concentrations in the leaves of plants grown from
CaCl-pretreated seeds increased significantly after
aphid attack. Furthermore, callose deposition was observed mainly in the
phloem. The results suggest that seed pretreatment with
CaCl primed the plant responses against wheat aphid
attack. The underlying mechanism comprises a comprehensive interaction
among Ca, callose regulation, and the expression of
callose synthesis-related genes, as well as phloem localization of
callose in response to aphid attack.
First detection and genetic characterization of a novel kirkovirus in diarrhoeal piglets in China
Authors: Tong Panpan , Ren Meiling , Wang Zunba , Song Xiaozhen , Zhang Lei , Song Guangwei , Jia Chenyang , Duan Ruli , Palidan Nuerlan , Kuang Ling , Xie Jinxin
Published: January 2024

In this study, a novel kirkovirus (kirV) was detected in anal swabs of
piglets with diarrhoea, the positive rate was 100% (149/149), but this
virus was detected in one of 261 clinically healthy piglets, this result
suggests a strong relationship between the kirV and the diarrhoeal
disease. We obtained the whole-genome sequences of three kirVs (named
Cj-D5, Cj-D32 and Cj-D43), with the length of 3750 nt, shared
86.6%–89.0% nt-identity with equine kirV Equ1 and kirV Cj-7-7.
Furthermore, three viruses share32.1%–93.4%, and 22.2%–27.1% of
the Rep protein sequence identity with available reference strains of
the proposed family Kirkoviridae, and the family Circoviridae,
respectively. Moreover, like kirV Equ1 and Cj-7-7, RCR domain and P-loop
NTPase domains of Rep protein, and nonanucleotide motif (CAATATTAC), of
three viruses, were similar to Circoviruses and Cycloviruses.
Phylogenetic analysis showed that these viruses were grouped together
with members in the proposed family Kirkoviridae. This is the first
report that kirV circulates in piglets with diarrhoea, and next studies
are needed to determine the pathogenesis of this virus.
Peptide OM-LV20 promotes structural and functional recovery of spinal cord injury in rats
Authors: Zhao Jian , Pang Ailang , Yang Meifeng , Zhang Xuemei , Zhang Rong , Liu Jingfei , Gu Yuanqi , Li Shanshan , Yin Saige , Hu Yan , Zhang Yue , Ba Yingchun , Meng Buliang , Yang Xinwang
Published: January 2024

At present, there are no satisfactory therapeutic drugs for the
functional recovery of spinal cord injury (SCI). We previously
identified a novel peptide (OM-LV20) that accelerated the regeneration
of injured skin tissues of mice and exerts neuroprotective effects
against cerebral ischemia/reperfusion injury in rats . Here, the
intraperitoneal injection of OM-LV20 (1 μg/kg) markedly improved motor
function recovery in the hind limbs of rats with traumatic SCI, and
further enhanced spinal cord repair. Administration of OM-LV20 increased
the number of surviving neuron bodies, as well as the expression levels
of brain-derived neurotrophic factor and its receptor tyrosine receptor
kinase B. In the acute stage of SCI, OM-LV20 treatment also increased
superoxide dismutase and glutathione content but decreased the levels of
malonaldehyde and nitric oxide. Thus, OM-LV20 significantly promoted
structural and functional recovery of SCI in adult rats by increasing
neuronal survival and BDNF and TrkB expression, and thereby regulating
the balance of oxidative stress. Based on our knowledge, this research
is the first report on the effects of amphibian-derived peptide on the
recovery of SCI and our results highlight the potential of peptide
OM-LV20 administration in the acceleration of the recovery of SCI.
An Automatic Annotation Model of Teeth and Dental Conditions in Dental Panoramic Radiographs: A Proposal of Segmentation Tool
Authors: Çelik Özer
Published: January 2024

Purpose: The study aimed to design and propose an automatic annotation
model of segmentation teeth in dental panoramic radiographs using
deep-learning artificial intelligence (AI) algorithms. Methods: A total
of 1183 anonymized panoramic radiographs were used to develop the AI
model (CranioCatch, Eskisehir, Turkey) for an automatic annotation model
of teeth and dental conditions including implant, crown, pontic, and
filling in dental panoramic radiographs. U-Net implemented with PyTorch
library was used for developing an automatic annotation model. The
confusion matrix was used as a metric of the success of the AI model.
Results: The sensitivity, precision, and F1-score values for teeth
annotation at 70% intersection over union (IoU) value were 1, 0.9894,
and 0.9947, respectively. At 50% IoU value, the sensitivity, precision,
and F1-score values were found as 1, 1, 1, respectively. The
sensitivity, precision, and F1-score values for dental condition
annotation at 70% IoU value were 0.9963, 0.84, and 0.9115,
respectively. At 50% IoU value, the sensitivity, precision, and
F1-score values were found as 0.9963, 0.9446, 0.9698, respectively.
Conclusion: To develop an artificial intelligence model for dental image
classification, image segmentation, and object detection generally needs
lots of labeled images. To overcome the struggles of manual annotation,
the proposed annotation tool can be used to make it easier to increase
the number of labeled data to further enhance the success of AI studies.
Predictive Performance of sFlt-1, PlGF and the sFlt-1/PlGF Ratio for Preeclampsia: A Systematic Review and Meta-Analysis
Authors: Ding Guifeng
Published: January 2024

Background: It is difficult to evaluate whether monitoring serum sFlt-1,
PlGF, or sFlt-1/PlGF in pregnant women who are suspected of having PE
can significantly shorten the PE diagnosis time. Objectives: To estimate
the accuracy of sFlt-1, PlGF and sFlt-1/PlGF in preeclampsia prediction.
Search Strategy: Databases including PubMed, Web of Science, Medline,
CNKI, SinoMed, VIP Journal, and Wanfang Data were searched for eligible
studies published until October 7, 2020. Selection Criteria: The
research subjects were pregnant women with or without PE. The research
types were case-control studies and cohort studies. This was an original
study involving the detection of at least one of the following in the
blood, serum or plasma: sFlt-1, PlGF, and sFlt-1/PlGF. Data Collection
and Analysis: Meta-Disc 1.4 was employed, using the Sen, Spe, PLR, NLR,
and DOR to plot SROC, and subgroup analysis and meta-regression were
conducted. Main Results: Meta-analysis showed that for sFlt, PlGF and
sFlt-1/PlGF, the Sen was 0.811 (95% CI: 0.783-0.837), 0.735 (95% CI:
0.713–0.757), and 0.779 (95% CI: 0.763–0.795), respectively; the Spe
was 0.786 (95% CI: 0.769-0.802), 0.731 (95% CI: 0.721-0.741),and 0.885
(95% CI: 0.881-0.889), respectively. Conclusions: The sFlt-1/PlGF ratio
showed better predictive performance for preeclampsia than sFlt-1 or
PlGF alone. However, the predictive value of the latter two cannot be
ignored. Funding: Xinjiang Uygur Autonomous Region “Tianshan Innovation
Team Plan”(2020D14010) Keywords: gestational hypertension;
hypertension; meta-analysis; preeclampsia; predictive; PlGF; sFlt-1;
sFlt-1/ PlGF, systematic review
Farrerol ameliorated renal fibrosis mediated by mitophagy deficiency in cisplatin-induced chronic kidney disease via Nrf2/PINK1
Authors: Ma Ning , HU Jianqiang , Gu Wenjing , Ci Xinxin
Published: January 2024

Background and Purpose: Mitochondrial dysfunction is essential in renal
tubular damage, and mitophagy, a selective form of autophagy,
specifically eliminates damaged mitochondria. Mitophagy reportedly
protects against diabetic kidney disease, cisplatin-induced acute kidney
injury (AKI) and other related kidney diseases, but the specific
mechanism by which mitophagy protects against cisplatin-induced chronic
kidney disease (CKD) remains unclear. Experimental Approach: The effects
of farrerol on cisplatin-induced AKI in mice were investigated. C57BL/6
wild-type and Nrf2 knockout mice were used to evaluate the protective
effect of farrerol on cisplatin-induced CKD. Key Results: we confirmed
that Nrf2- and PINK1/Parkin-mediated mitophagy was significantly
increased on the 3rd day of cisplatin stimulation but was reduced on the
38th day of cisplatin stimulation. Similar to previous results,
farrerol, a natural compound, also activated Nrf2 on the 38th day of
cisplatin administration, subsequently stimulating the Nrf2-targeted
antioxidant enzymes HO-1 and NQO1. In addition, farrerol triggered
PINK1/Parkin-mediated mitophagy by recruiting the receptor proteins LC3
and p62/SQSTM1, thereby eliminating damaged mitochondria. Furthermore,
genetic deletion of Nrf2 reduced PINK1/Parkin-mediated mitophagy
activation and led to increased renal tubular necrosis and renal
fibrosis. We also found that farrerol alleviated inflammation and renal
fibrosis by inhibiting p-NF-κB/NLRP3 and TGF-β/Smad signaling.
Conclusions: These data indicated that farrerol effectively inhibited
cisplatin-induced inflammation and renal fibrosis by activating Nrf2 and
PINK1/Parkin-mediated mitophagy, which provides a potential novel
therapeutic target for CKD.
Performance of the Cloud DX connected HealthKit Pulsewave in home blood pressure monitoring in a pregnant population: a clinical evaluation and user experience study
Authors: Hofstede Aniek , Lomme Mayke , Gosselink Stefanie , Drongelen Joris van
Published: January 2024

Objective: Clinical evaluation and user experience of the Cloud DX
connected HealthKit Pulsewave wrist cuff blood pressure monitor (CDXP)
for home blood pressure monitoring during pregnancy (HBPM). Methods: In
the first phase, an adjusted version of the European Society of
Hypertension International Protocol Revision 2010 (ESH-IP 2010) was used
to compare the CDXP and Omron M6 Comfort to the aneroid manometer. In
the second phase, blood pressure measurement at home was compared to
standard office blood pressure measurement (OBPM). Patients filled out a
questionnaire regarding user experience. Results: In 34 pregnant women
the blood pressure measured by the aneroid manometer did not differ from
the CDXP (systolic blood pressure (SBP) difference: -0.7±6.5 mmHg
(p=0.38), diastolic blood pressure (DBP) difference: 0.4±5.7 mmHg
(p=0.55)), while the aneroid manometer measured SBP slightly higher
(1.5±5.8 mmHg (p=0.04)) and DBP slightly lower (-2.8±5.8 mmHg
(p<0.01)) as compared to the Omron M6 Comfort. In 32 patients
the SBP of the office hospital measurement was significantly higher,
while DBP was comparable. The mean user experience score for the CDXP
was 85%, corresponding with an ‘excellent’ evaluation. Of all patients,
97% reported they liked the idea of home monitoring and would like to
use it in the future. Conclusion: The CDXP is as reliable as standard
office blood pressure monitors during pregnancy and gives a better
representation of the blood pressure than standard hospital measurement.
Pregnant women are positive and confident about HBPM and find the CDXP
easy to use.
TOXICITY PROFILE OF PARENTERAL ARTESUNATE FOLLOWING SUBACUTE TREATMENT IN RATS AND DOGS
Authors: Xiong Yanjie , Lang Johanna , Thun-Battersby Susanne , Huang Qin
Published: January 2024

OBJECTIVES The objectives of these studies were to investigate the
toxicity, safety and toxicokinetics of single and multiple doses of
artesunate for injection in rats and dogs. METHODS Sprague-Dawley rats
and Beagle dogs were treated intravenously or intramuscularly for 28
consecutive days with doses of up to 30 mg/kg artesunate, evaluating
toxicity, kinetics, genotoxicity, and cardiovascular and central nervous
safety parameters after single and 4-week repeated administrations.
Furthermore, respiratory parameters were evaluated after a single
intravenous administration in rats. RESULTS Artesunate was well
tolerated with no mortality and only minor effects on clinical pathology
parameters. Following repeated intramuscular administration, local
reactions at the injection site became evident. Signs of regenerative
anaemia were evident in both rats and dogs and are attributed to the
pharmacological effect of artesunate (effective against blood stages of
malaria parasites). No severe toxicity or any effects on safety measures
were noticed. CONCLUSIONS The results obtained in these studies support
the safe use of intravenous and intramuscular artesunate for a period
beyond the commonly used three (to maximum seven) days in humans.
Cardiovascular, central nervous and respiratory safety measures indicate
no risk at clinically used doses.
A novel approach to Hayman suture technique in the management of severe postpartum hemorrhage: Three vertical uterine compression sutures
Authors: Ozdemir Ismail
Published: January 2024

To reduce maternal morbidity and mortality due to bleeding caused by
uterine atony, a simple and effective procedure that can be easily
applied even junior physicians is required. Two vertical sutures are
transfixed, one on each side of the lower uterine segment, from anterior
to posterior over the bladder reflection avoiding the endometrial
cavity, and tied over the fundus. Similarly, a third is placed between
the two vertical sutures at the same level and tied in the middle of the
fundus. We report a novel approach of using three vertical compression
sutures in 25 women without requiring hysterectomy except one.
Strength of clinical evidence leading to approval of novel cancer medicines in Europe: a systematic review and data synthesis.
Authors: farina alberto , Moro Federico , Fasslrinner Frederick , Sedghi Annahita , Bromley Miluska , Siepmann Timo
Published: January 2024

Aim We aimed to evaluate the quality of clinical evidence that
substantiated approval of cancer medicines by European Medicines Agency
(EMA) in the last decade. Methods We performed a systematic review and
data synthesis of EMA documents in agreement with PRISMA guidelines. We
included European Public Assessment Reports, Summaries of Product
Characteristics, and published randomized controlled trials (RCTs) on
anti-cancer drugs approved by EMA from 2010 to 2019, and excluded drugs
not indicated for targeting solid or hematological tumors and
non-innovative treatments. We synthesized frequencies of approvals
differentiating between unblinded and blinded RCTs with and without
overall survival (OS) as predefined primary outcome measure. We assessed
frequency of post-approval RCTs for indications without at least one RCT
at the time of approval. Results Of 199 approvals, 159 (80%) were
supported by at least one RCT, 63 (32%) by at least one RCT having OS
as the primary or co-primary endpoint, 74 (37%) by at least one blinded
RCT, and 30 (15%) by at least one blinded RCT having OS as the primary
or co-primary endpoint. Whereas 40 approvals (20%) were not supported
by any RCT and, of those, 9 (22%) were followed by a post-approval RCT.
Discussion While the majority of approvals of cancer medicines approved
by EMA was supported by at least one RCT, we noted substantial
methodological heterogeneity of the studies.
THE EFFECTS OF ONLINE EXERCISE PROGRAM ON FATIGUE, BENEFITS AND QUALITY OF LIFE IN WOMENS DURING COVID-19 LOCKDOWN
Authors: Senbursa Gamze , Canarslan Busra , Yakut Yavuz , Baltaci Gul
Published: January 2024

Abstract Objective: Live-based exercise programs offer great potential
for the implementation of evidence-based interventions focused on
promoting healthy habits. The outbreak of COVID-19 provided an
opportunity to undertake an online survey to study the changes in
exercise barriers/benefits, fatigue level, and quality of life after
online live exercise program during lockdown, or quarantine. Methods:
The sample was composed of a total of 143 women (age Mean =
32.48±6.6yrs) from a convenience sample of participants was recruited
through social media (e.g. Facebook, Twitter, WhatsApp). The
questionnaire asked for demographic information, body weight, body
height, physical activity, and lifestyle factors before and during the
quarantine. One-hundred and forty-three women volunteers were received
an online live exercise program including strengthening, HIIT and
stretching 6 sessions in a week during COVID19 pandemic and were
assessed the measures of recruitment, exercise benefits/barriers,
fatigue severity level and general quality of life at baseline, 3rd-wk
and 6th-wk of the exercise program. Results: Significant differences
were observed in fatigue severity scale and health status between
baseline and at 3rd-wk (p < 0.05) in women. No difference in
the level of fatigue, barriers, benefits of exercise, quality of life
and health status between baseline and at 3rd-wk, and 3rd-wk and 6th-wk
or baseline and 6th-wk (p>0.05). Conclusion: This study
demonstrates that the internet-based exercise program is a viable
alternative for the delivery and dissemination of interventions focused
on promoting healthy habits, and a totally self-administered
intervention can also produce short-term positive results. This program
supports during lockdown reduce the risk of developing persistent
fatigue related chronic fatigue syndrome.
A novel sustainable honeytrap device for removal of Pseudomonas from water
Authors: Ranade Hemangi , Paliwal Priya , Rathore Deepshikha , Datta Manali
Published: January 2024

This paper introduces a novel prototype for removal of Pseudomonas from
water samples. Bacterial cells have the tendency to get attracted
towards species specific chemicals justifying a phenomenon known as
chemotaxis. We designed a strip integrating a combination of serine, a
pseudomonas specific chemo-attractant and honey to attract and inhibit
the bacteria in situ. Upon dipping the serine side of the strip,
pseudomonal cells were attracted towards it. The porous property of the
strip facilitates the ‘trapping’ and subsequent diffusion of the
bacterial cells towards honey-adsorbed end of the strip. Honey, a
natural antimicrobial agent, has garnered attention in its effective
inhibitory role in Pseudomonal biofilms and wound infections. This
‘honeytrap’ reportedly leads to targeted elimination of Pseudomonas,
hence facilitating its removal from water. The percentage removal
efficacy of this ‘honeytrap’ device is 96% with a log reduction
equivalent to 1.6 within a time frame of 2 hours. Pseudomonas
aeruginosa, although, not a natural contaminant of the potable water,
enters circulation due to improperly maintained plumbing fixtures and
storage facilities. P. aeruginosa has been implicated as a causative
agent in gastroenteritis, keratitis, endocarditis, urinary tract
infections and septicemia amongst many, thus, making it a potent health
risk. Honeytrap strip is an easy to use, biodegradable and
cost-effective sustainable solution, which can passively neutralize
pseudomonas from water. The scaled-up version of honeytrap device may
help us in achieving better outcomes in terms of potable water with
substantial improvement in quality of life.
Changes in Therapeutic Profile Overtime of Women Diagnosed with Breast Cancer in a Tertiary Hospital
Authors: Merchán-Galvis Angela , Gama Karla Salas , Requeijo Carolina , Jofra Laura Samsó , Canovas Esther , Quintana M. Jesús , Xavier Bonfill Cosp
Published: January 2024

Changes in Therapeutic Profile Overtime of Women Diagnosed with Breast
Cancer in a Tertiary Hospital
Hearing performance after myringoplasty with full-thickness cartilage: A propensity score-matched cohort study
Authors: Huang Yi-bo , Xu Jiang-Hong , Pan Yu-Cheng , Lv Ji-Han , Yuan Ya-sheng , han zhao , Ren Dong-dong
Published: January 2024

Objective: Hearing performance after myringoplasty with full-thickness
tragal cartilage (FTTC) has been questioned because a considerable
difference exists between the graft and natural tympanum. This study
aimed to analyze the air-bone gap (ABG) trends before and after
myringoplasty and its interaction with risk factors in the FTTC group;
further, it compared postoperative ABG in the FTTC group to that in the
temporalis fascia (TF) and partial-thickness tragal cartilage (PTTC)
groups. Design: A retrospective cohort study Setting: Tertiary care
University Hospital Methods: The general linear model repeated measures
was used to analyze ABG trends in the FTTC group and its influencing
factors. Non-parametric tests were used to compare ABGs in the FTTC
group and the other two groups. Propensity score matching was done to
balance baseline characteristics between the TF and FTTC group. Results:
The mean postoperative ABG in the FTTC group was 12.17±6.58 dB, and
90.9% of the patients had a mean ABG within 20 dB, which improved
significantly after surgery (F(1,108)=38.707, P<.001). The
tympanic perforation size and malleus handle exposure status, which
significantly affected the preoperative ABG, did not affect
postoperative hearing (P>.05). In the FTTC group, the mean
ABG was comparable to that in the other two groups (P>.05).
For all except 250 Hz, a similar audiological performance was observed
in the FTTC and PTTC groups; FTTC had a better performance at 250 Hz
(Z=-2.277, P=.023). Conclusions: Good hearing performance was achieved
after myringoplasty with FTTC, irrespective of the preoperative
perforation size and malleus handle exposure status.
Huge Left Atrial Appendage Aneurysm: A Case Report and Review of the Literature
Authors: Li Daowei , Ding Mingyan , Zhao Hanzhang , Zhu Fang
Published: January 2024

Background: Left atrial appendage aneurysm (LAAA) is a rare cardiac
disease with potentially life-threatening complications such as systemic
thromboembolism, atrial tachyarrhythmia and cardiac dysfunction.
Currently, early surgical intervention is recommended as the most
suitable treatment to prevent these complications. Case introduction: In
this case report, we describe a 58-year-old female patient admitted to
the hospital with the main symptoms of active dyspnea and recurrent
palpitations for 3 years, which had worsened for a week prior to
presentation. The relevant literature was also reviewed to summarize the
clinical features of LAAA and physical examination and diagnostic
work-ups for patients, including those with no apparent symptoms. These
findings will provide valuable information for further treatment options
and facilitate necessary intervention measures to prevent serious
complications.
Short-term exposure to high temperature and salinity altered dead pericarp properties and diminished yield of the crop plant Brassica juncea
Authors: Swetha Bupur , Singiri Jeevan , Novoplansky Nurit , Grandhi Rohith , Srinivasan Jansirani , Khadka Janardan , Sikron-Persi Noga , Galis Ivan , Grafi Gideon
Published: January 2024

Climate change is expected to increase the frequency and severity of
abiotic stresses (e.g., hot spell, salinity, drought), which might lead
to loss of crop yield. We investigated the effect of salinity (S), short
episodes of high temperature (HS) and combination of S+HS at the
reproductive phase on seeds and dead pericarps properties of the crop
plant Brassica juncea. Three interval exposures to HS resulted in
massive seed abortion, and seeds from salt-treated plants germinated
poorly. HS significantly reduced metabolites accumulated in dead
pericarps, except for upregulation of isomaltose and cellobiose. Salt
induced alteration in metabolite levels including increase in proline,
reduction in TCA intermediates and changes in phytohormone levels.
Proteome analysis revealed hundreds of proteins stored in dead pericarps
whose levels and composition were altered under salt stress. The
integration of metabolic and proteomic data showed that changes in
metabolites were highly correlated with changes in proteins involved in
their biosynthetic pathways. Thus, dead pericarps store beneficial
substances whose level and composition are altered under stress
conditions. The results highlighted the detrimental effect of short
episodes of HS during the reproductive phase on crop production, which
might have implications for global food security in the face of climate
change.
Investigation of the effect of PEG detoxification on diphtheria vaccine
Authors: Hesari Tahere , Tahoori Fatemeh , Nazari Ali , Najafabadi Zahra Salehi , Samianifard Maedeh , Faramarzi Ardeshir , Soleimani Majid
Published: January 2024

Immunization has been considered as a successful global health program
that saves life of many persons each year. The vaccines reduce the risk
of getting the disease by providing immunity in the body. Therefore, the
constant availability of essential vaccines is an important factor in
the health of the community. One of the most important vaccines
worldwide is the diphtheria vaccine, which is usually given multivalent
with tetanus and pertussis. It takes about 45 days to produce this
vaccine from the time of initial culture to the production of the
toxoid. If it is possible to carry out many stages of production under
normal conditions and maintain the chemical intermediate, it will be a
great help to produce this vaccine. In this study, the method of
purification (by washing and chromatography) and storage of toxins for
several months is presented. In this method, by concentrating the
sample, it can be stored in a smaller space and there will be no problem
in providing a suitable space. Another problem with the diphtheria
vaccine is that it is reversible after detoxification of the toxin using
formaldehyde. In order to solve this problem, it is suggested to use
mPEG for detoxification, which will produce more stable covalent bonds
between PEG and the first type of amine groups (-NH2) in the toxin
chain. Therefore, we will have a stable product that the results of
animal tests show that this product caused no wound or necrosis in the
tested animals.
Combining QTL mapping and transcriptomics to decipher the genetic architecture of phenolic compounds metabolism in the conifer white spruce
Authors: Laoué Justine , Depardieu Claire , Gérardi Sébastien , Lamothe Manuel , Bomal Claude , Azaiez Aida , Gros-Louis Marie-Claude , Boyle Brian , Hammerbacher Almuth , Isabel Nathalie , Bousquet Jean
Published: January 2024

Conifer forests worldwide are becoming increasingly vulnerable to the
effects of climate change. Although phenolic compounds (PCs) have been
shown to be modulated by biotic and abiotic stresses, the genetic basis
underlying the variation in their basal composition remains poorly
documented in conifers. We used QTL mapping and RNA-Seq to explore the
complex polygenic network underlying the constitutive production of PCs
in white spruce (Picea glauca) progeny for two years. QTL detection was
performed for nine PCs and differentially expressed genes (DEGs) were
identified between individuals with high and low PC contents for five
PCs exhibiting stable QTLs across time. A total of 17 QTLs were detected
for eight metabolites, including one major QTL explaining up to 91.3%
of the neolignan-2 variance. The RNA-Seq analysis highlighted 50 DEGs
associated with phenylpropanoid biosynthesis, several key transcription
factors, and a subset of 137 genes showing opposite expression patterns
in individuals with high levels of the flavonoids gallocatechin and
taxifolin glucoside. A total of 19 DEGs co-localised with QTLs. Our
findings represent a promising step towards resolving the genomic
architecture of PC production in spruce and facilitate the functional
characterization of genes and transcriptional networks responsible for
differences in constitutive production of PCs in conifers.
Graph-theoretic method on topology identification of stochastic multi-weighted complex networks with time-varying delayed coupling and non-delayed coupling
Authors: Chen Huiling , Zhang Chunmei , Xu Qin , Feng Yuli
Published: January 2024

In this paper, the aim is to identify uncertain topological structures
of stochastic multi-weighted complex networks (SMWCN), in which both
time-varying delayed coupling and non-delayed coupling are considered.
Distinguished from the previous work, graph theory and Lyapunov method
are combined to research topology identification of SMWCN. Combining
LaSalle-type invariance principle for stochastic differential equations
and some strategies of inequalities, theoretical results about topology
identification and partial topology identification of SMWCN are obtained
under adaptive control and adaptive pinning control, respectively.
Furthermore, by using the drive-response concept, response network can
reach synchronization with drive network. In the end, two numerical
simulations are provided to demonstrate the effectiveness and
correctness of theoretical results, in which typical
L\”{u} chaotic system is used to describe the vertex’s
dynamical behavior.
NUMERICAL SOLVING OF A 3-D STIFFNESS MATRICES OF THE WOOD 1 BEAM USING THE FINITE ELEMENT METHOD
Authors: Bikanda louis , LISSOUCK René OUM , MASSAGA Junior Morel ANGOUAH , OHANDJA Louis Max AYINA , NGA Hippolyte NTEGE
Published: January 2024

The interest of this article is to show the relevance of the non-linear
geometric model to estimate and to simulate numerically the critical
wooden beams buckling load. Base on the kinematic hypotheses of the
Vlassov’s theory, the consideration of the kinematic relationships
associated with warping are presented in a coherent and unified
geometric form. The virtual work principal in updated lagrangian
description allowed us to determine the incremental equations of
equilibrium. Polynomial interpolation helps us to reconstruct the main
matrices of the problem of nonlinear geometry. The size of the rigidity
matrices obtained takes into account the warping phenomenon. In a
numerical plan, the resolution of the equations established allows us to
appreciate relevance of the seventh degree of freedom on predicting the
critical buckling load.
Zoo-sanitary situation assessment, a preliminary step for country disease prioritization approach? Systematic review and meta-analysis from 2000 to 2020 in Cameroon.
Authors: Mouiche-Mouliom Mohamed-Moctar , Wafo Eugenie Elvire Nguemou , Mpouam Serge Eugene , Moffo Frédéric , Feussom Jean Marc Kameni , Ngapagna Arouna Njayou , Mfopit Youssouf , Abdoulmoumini Mamoudou
Published: January 2024

Infectious diseases of livestock are a significant burden on animal and
human health, particularly in Low and Middle-Income Countries. To
prevent and control these diseases, an appropriate surveillance system
based on suitable up-to-date epidemiological data is required. This
systematic review looks at the available data on infectious diseases of
livestock in Cameroon from 2000-2020 gives an epidemiological
contribution for effective control strategies and initial step for
disease prioritization approach. The systematic review protocol was
designed according to the PRISMA statement. Data was searched through
online databases. Grey literature comprised of dissertations and thesis
from veterinary higher education institutions in Cameroon. A
random-effects model was used to calculate pooled prevalence using
Comprehensive Meta-analysis software. Major infectious diseases of
livestock in Cameroon based on prevalence were gastrointestinal
parasitosis (57.4% in cattle, 67.2% in poultry, 88% in pigs),
hemoparasites (21.6% in small ruminants, 19.7% in cattle), bovine
pasteurellosis (55.5%), fowl salmonellosis (48.2%), SRP (39.7%), FMD
(39.4% in cattle) and ASF (18.9%). Furthermore, other important
endemic zoonoses in the country included: bovine tuberculosis (3.3% in
cattle), brucellosis (7% in cattle, 8% in pigs), bovine leptospirosis
(2.5%), RVF (10.9% in cattle, 3.7% in small ruminants) and hepatitis
E virus (8.4% in pigs). Most of the retrieved research were carried out
in the Adamawa, North-West and West regions. The high-pooled prevalence
of various livestock infectious diseases and evidence for the endemicity
of major zoonotic infections in Cameroon warrants for enhanced public
health interventions strategically targeting prioritized animal diseases
for surveillance and control by making sure all diseases that result in
production losses, negative economic impacts and public health threats
are epidemiologicaly appropriately characterised in all production
systems. The evaluation of existing data, albeit publication-specific,
is an important step in the process of prioritising animal diseases,
including zoonosis.
Pregnancies in women with pulmonary hypertension remain challengeable: a single-center experience from East China
Authors: Miao Huixian , Chen Yunyan , Wang Chuan , Huang Taotao , Lin Jianhua
Published: January 2024

In this study, we aim to find out maternal and fetal outcomes in women
with PH resulting from different causes and try to determine the risk
factors related to adverse maternal and fetal outcomes. 684 women were
included in the final analysis, who were mainly had pulmonary
hypertension secondary to congenital heart disease (65.4%). Patients
with idiopathic PH have the highest mortality (35.3%) and the lowest
live birth rate (82.4%). Mortality is similar between moderate and
severe PH women(5.3% vs 9.7%, P=0.17), but live birth rate is much
lower in severe PH women (78.6% vs 89.9%, P＜0.01). The overall
mortality was 2.8%, decreasing from 6.6% (4/61) in
2001~2005 to 1.7% (4/235) in 2016~2020
(P=0.10). Hypoxemia and vaginal birth are risk factors of maternal death
both in the univariate analysis [OR 13.49(5.15-35.31), OR
4.16(1.52-11.42), respectively] and multivariate analysis [35.28
(1.42-878.75), 850.86 (5.67-127606.74), respectively]. General
anesthesia is a risk factor in the univariate analysis [OR
12.07(3.72-39.15)] but with no significance in the multivariate
analysis (P=0.16). We demonstrate a lower mortality rate than previously
reported, although of no statistical difference among years in our
center. In PH secondary to connective tissue disease and idiopathic PH,
maternal mortality remains high. Maternal and fetal outcome are related
to the etiology and the severity of PASP. Hypoxemia is a risk factor of
maternal death while cesarean section seems to be a protective factor.
The ideal choice of anesthesia remains uncertain. PH-targeted drugs
cannot effectively decrease mortality rate. Thus, pregnancies remain
challengeable in PH women.
Inhibition of c-Fos expression attenuates IgE-mediated mast cell activation and allergic inflammation by counteracting an inhibitory AP1/Egr1/IL-4 axis
Authors: Wang Hui-Na , Ji Kunmei , Zhang Li-Na , Xie Chu-Chu , Li Wei-Yong , Zhao Zhen-Fu , Chen Jia-Jie
Published: January 2024

Background: Activator protein-1 (AP1), a c-Fos–JUN transcription factor
complex, mediates many cytobiological processes. c-Fos has been
implicated in immunoglobulin (Ig)E activation of mast cells (MCs) via
high-affinity IgE Fc receptor (FcεRI) binding. This study examined c-Fos
involvement in MC activation and tested the effects of the c-Fos/AP1
inhibitor T-5224 on MCs activation and allergic responses. Methods: In
vitro studies were conducted with two MC model systems: rat basophilic
leukemia cells (RBLs) and mouse bone marrow derived mast cells (BMMCs).
MC degranulation and effector functions were examined with
β-hexosaminidase release and cytokine secretion assays. c-Fos/AP1 was
inhibited with T-5224. c-Fos activity was suppressed with short hairpin
RNA targeting c-Fos (shFos). In vivo immune responses were evaluated in
passive cutaneous anaphylaxis (PCA) and ovalbumin-induced active
systemic anaphylaxis (ASA) models, as well as in an oxazolone
(OXA)-induced model of atopic dermatitis, a common allergic disease.
Results: c-Fos expression was elevated transcriptionally and
translationally in IgE-stimulated MCs. c-Fos binding of the Egr1 (early
growth response 1) promoter upregulated Egr1 transcription, leading to
production of interleukin (IL)4. T-5224 reduced FcεRI-mediated MC
degranulation (evidenced by β-hexosaminidase activity and histamine
levels) and diminished EGR1 and IL4 expression. T-5224 attenuated
IgE-mediated allergic responses in PCA and ASA models, and it suppressed
MC-mediated atopic dermatitis in mice. Conclusion: IgE binding can
activate MCs via a c-Fos/Egr1/IL-4 axis. T-5224 suppresses MC activation
in vitro and in vivo and thus represents a promising potential strategy
for targeting MC activation to treat allergic diseases.
Telegraph model with fractional differential operators: Nonsingular Kernels
Authors: Mishra Jyoti
Published: January 2024

Very recently, Atangana and Seda made use of the Newton polynomial
interpolation to suggest new numerical scheme to solve linear and
non-linear problems. In this paper, we applied such numerical method to
solve the well-known telegraph equations with different differential
operators. The stability analysis of each case is presented in detail
and numerical simulations are presented for different values of
fractional orders and fractal dimensions.
The study of laccase immobilization optimization and stability improvement on CTAB-KOH modified biochar
Authors: Wang Zhaobo , Ren Dajun , Jiang Shan , Yu Hongyan , Cheng Yaohui , Zhang Shuqin , Zhang Xiaoqing , Chen Wangsheng
Published: January 2024

In this paper, cetyltrimethylammonium bromide (CTAB)-KOH modified
biochar (CKMB) was used to immobilize laccase by adsorption method
(laccase@CKMB). Based on the results of the single-factor experiments,
the optimal loading conditions of laccase@CKMB were studied with the
assistance of Design-Expert 12 and response surface methods. The
predicted optimal experimental conditions were laccase dosage 1.78
mg/mL, pH 3.1 and 312 K. Under these conditions, the activity recovery
of laccase@CKMB was the highest, reaching 61.78 %. Then, the CKMB and
laccase@CKMB were characterized by TGA, FT-IR, XRD, BET and SEM, and the
results showed that laccase could be well immobilized on CKMB, the
maximum enzyme loading could reach 57.5 mg/g. Compared to free laccase,
the storage and pH stability of laccase@CKMB was improved greatly. The
laccase@CKMB retained about 40 % of relative activity (4 ℃, 30 days)
and more than 50 % of relative activity at pH 2-6. In addition, the
laccase@CKMB indicated the reusability up to 6 reaction cycles while
retaining 45.1 % of relative activity. Moreover, the thermal
deactivation kinetic studies of laccase@CKMB showed a lower k value
(0.00275 min-1) and higher t1/2 values (252.0 min) than the k value
(0.00573 min-1) and t1/2 values (121.0 min) of free laccase. In summary,
we explored scientific and reasonable immobilization conditions of
laccase@CKMB, and the laccase@CKMB possessed relatively better
stabilities, which gave the immobilization of laccase on this cheap and
easily available carrier material the possibility of industrial
applications.
Synthesis, crystal structure, vibrational properties and DFT studies of 1-(4-((6-bromopyrido [2, 3-d]pyrimidin-4-yl)oxy)phenyl)-3-(2,4- difluorophenyl)urea
Authors: Sun Hong , Deng liyuan , Hu weiyin , Zhou zhixu , Chai Huifang , Zhao chunshen
Published: January 2024

In this present work, the title compound
1-(4-((6-bromopyrido[2,3-d]pyrimidin-4-yl)oxy)
phenyl)-3-(2,4-difluorophenyl)urea (1) was synthesized and the structure
was characterized by spectroscopy (FT-IR, 1H NMR, 13C NMR and MS). The
single crystal of the title compound was confirmed by X-ray diffraction,
and the optimized molecular crystal structure was determined based on
DFT calculations using B3LYP/6-311G (2d, p) basis set, which was
compared with the X-ray diffraction. The results of the conformation
analysis indicated that the molecular structure optimized by DFT was
consistent with the crystal structure determined by X-ray single crystal
diffraction.