bluuebunny/crossref_metadata_2025 huggingface dataset:
Robotic-assisted sleeve gastrectomy with simultaneous Roux-en-Y cystojejunostomy in a patient with sever obesity and a pancreatic pseudocyst: a case report
Authors: Zhang Zheng , Wang Lun , Wei Zhiqiang , E Changyong , Jiang Tao
Published: January 2024

Introduction

We tried to apply a new surgical method to treat obesity combined with pancreatic pseudocyst and achieved satisfactory results.

Case and presentation

We report a case of a severely obese patient with pancreatic pseudocyst who underwent robotic-assisted sleeve gastrectomy, while the pseudocyst was incised and cyst-jejunostomy was performed. The operation was successful, and the patient was discharged on the 8th day after the procedure. There were no complications during the perioperative period. After 12 months of follow-up examinations, the patient's pancreatic pseudocyst disappeared. Additionally, there was a significant decrease in body weight, body mass index, and other indicators. As a result, obesity and related metabolic diseases were completely relieved.

Conclusions

This case summarizes and presents the experience of using robotic bariatric surgery for the treatment of pancreatic pseudocyst. This case report indicates that this surgical procedure is both safe and effective for patients with pancreatic pseudocyst who also have obesity and related metabolic diseases.
Inflammatory biomarker concentrations in dogs with gastric dilatation volvulus with and without 24-h intravenous lidocaine
Authors: Brunner Anna , Lehmann Anna , Hettlich Bianca , Peters Laureen M. , Doras Camille Julie , Adamik Katja-Nicole
Published: January 2024

Background

Canine gastric dilatation volvulus (GDV) is characterized by tissue ischemia, reperfusion, and systemic inflammation. Evidence exists that lidocaine exerts anti-inflammatory properties and potentially improves outcome.

Design and setting

Prospective, randomized observational cohort study in client-owned dogs with GDV.

Objective

The primary objective of the study was the determination of pro- and anti-inflammatory biomarker concentrations in dogs with GDV with and without intravenous (IV) lidocaine therapy. The second objective was the evaluation of side effects of lidocaine.

Methods

Of 35 dogs included in the study, 20 dogs were assigned to receive lidocaine (LIDO) (2 mg/kg initially, followed by a continuous infusion at a rate of 50 μg/kg/min over 24 h) and 15 dogs not to receive lidocaine (NO-LIDO). Plasma concentrations of cytokines interleukin (IL)-6, IL-7, IL-8, IL-10, IL-15, IL-18, interferon gamma, keratinocyte chemotactic-like, monocyte chemotactic protein, and C-reactive protein (CRP) were measured at admission (prior any therapeutic intervention, T0), immediately after surgery (T1), at 24 h (T24), and at 48 h (T48) post-surgery.

Results

No significant differences in concentrations of any cytokines were found between the LIDO- and the NO-LIDO group. Significant lower CRP concentrations (median [range]) were found in dogs with lidocaine compared to dogs without at T24 (97.5 pg/mL [46.3–161.7] vs. 127.9 pg/mL [26.9–182.0];p = 0.046) and T48 (73.7 pg/mL [18.4–169.4] vs. 116.3 pg/mL [71.4–176.8];p = 0.002). Dogs receiving lidocaine exhibited significantly impaired mentation, a prolonged period of anorexia, and longer hospitalization compared to dogs without lidocaine.

Conclusion

Lidocaine administration had no impact on the plasma levels of cytokines during the 48-h study period, but significantly lower CRP concentrations were found at T24 and T48. Lidocaine’s potential side effects require careful decision making regarding its use.
Multimodal Management of Colorectal Liver Metastases: State of the Art
Authors: Filoni Elisabetta , Musci Vittoria , Di Rito Alessia , Inchingolo Riccardo , Memeo Riccardo , Mannavola Francesco
Published: January 2024

Liver is the most common site of colorectal cancer (CRC) metastases. Treatment of CRC liver metastases (CRLM) includes different strategies, prevalently based on the clinical and oncological intent. Valid approaches in liver-limited or liver-prevalent disease include surgery, percutaneous ablative procedures (radiofrequency ablation, microwave ablation), intra-arterial perfusional techniques (chemo-embolization, radio-embolization) as well as stereotactic radiotherapy. Systemic treatments, including chemotherapy, immunotherapy and other biological agents, are the only options for patients with no chance of locoregional approaches. The use of chemotherapy in other settings, such as neoadjuvant, adjuvant or conversion therapy of CRLM, is commonly accepted in the clinical practice, although data from several clinical trials have been mostly inconclusive. The optimal integration of all these strategies, when applicable and clinically indicated, should be ever considered in patients affected by CRLM based on clinical evidence and multidisciplinary experience. Here we revised in detail all the possible therapeutic approaches of CRLM focusing on the current evidences, the studies still in progress and the often contradictory data.
“I Prefer Eating Less Than Eating Healthy”: Drivers of Food Choice in a Sample of Muslim Adolescents
Authors: Nield Lucie
Published: January 2024

Adolescence is a time of significant change which is experienced differently across sociocultural contexts. Understanding drivers of food practice in adolescence is vital as these impact future health outcomes and can drive health inequality. This study investigates drivers of food choice in predominantly British–Asian, Muslim adolescents living in a deprived urban area of England. It identifies dominant drivers of food choice and their impact on health and wellbeing, and explores how Muslim adolescents from a low socioeconomic, British–Asian group understand and action autonomy in their food practices. PhotoVoice, a focussed ethnographical methodology where participants reflect on lived experience, was used to address the study aim. Participants (n = 21) were secondary school pupils aged 14–15 years, recruited from a school situated in an area of deprivation. Four overarching themes were developed from the qualitative data framework analysis: (1) food preference and other determinants of food choice; (2) concept, understanding and importance of health; (3) developing autonomy, skills, and independence; and (4) role of community, friends, and family in food practices. The adolescents were developing autonomy in relation to their food practices, whilst navigating a complex web of factors which were, in part, determined by their social class location and demographics. Participants understood the constituents of healthy eating. However, there was a perceived “effort” of being healthy, including additional time for preparing healthier food and sacrificing taste preferences. Parents, friends, and schools highly influence food choices, with adolescents preferring a broad palate of takeaway and convenience foods and would prefer to eat less of these “unhealthy” options than eat healthily.
Impact of Agricultural Cooperatives on Farmers’ Collective Action: A Study Based on the Socio-Ecological System Framework
Authors: Zhu Xiaoyan , Wang Guangyao
Published: January 2024

Agricultural cooperatives greatly influence agricultural and rural modernization in China. Based on 381 farmer samples in the arid Tarim River Basin, this empirical study aimed to construct an index system for the exploration of the relationship between cooperatives and farmers’ collective action by using the Socio-Ecological System framework. The results showed that agricultural cooperatives helped to empower farmers to act collectively. Agricultural cooperatives, with the mechanisms of collective decision making, institutional constraints, and internal supervision, could realize the integration of resources required for farmers’ collective action and promote the sharing of risks and benefits. By providing financing support and a platform for resource integration, cooperatives could reduce constrains induced by economic difference among farmers; enhance village leadership, organization, and coordination; and promote the accumulation of social capital and villagers’ sense of identity with the village. Particularly, cooperatives could support farmers to adopt water-saving irrigation technologies and reduce their over-dependence on chemical pesticides and fertilizers, thus promoting farmers’ collective action. Therefore, the development of agricultural cooperatives will help enhance farmers’ collective action, promote the modernization of rural governance, and realize rural revitalization.
Dynamics of Agronomic Characteristics and Plant Diversity in Lemon Verbena (Aloysia citrodora Paláu) Cultivation in Greece
Authors: Molla Aikaterini , Solomou Alexandra D. , Tziouvalekas Miltiadis , Lolas Alexios , Skoufogianni Elpiniki
Published: January 2024

Nowadays, there is an increasing negative environmental effect of using chemical fertilizer. For this reason, the use of biofertilizers is promoted in the agriculture sector. The purpose of this investigation was to carry out an evaluation of the effects of biological fertilizer (biofertilizers are organic materials that can be used to improve soil properties) use on the growth and agronomic characteristics of Aloysia citrodora. To achieve this aim, a two-year randomized complete blocks field experiment was carried out in central Greece. The experiment included four biological fertilizer levels (0, 50, 100 and 150 N kg ha−1) with three replications. Plant height, dry stem, dry leaves, dry total yield and leaf area index (LAI) were measured during the two growing years. The results showed that in the first and second studied years, the maximum plant height and total dry yield were observed in the BF150 treatment. Moreover, LAI was ameliorated by applying the BF100 and BF150 treatments in both studied years. A key finding in this study is that the A. citrodora ecosystem favors herbaceous plant species richness. Also, soil factors (soil organic matter, P and K) promote positive herbaceous plant diversity within the A. citrodora ecosystem. Finally, A. citrodora could be a promising medicinal plant, cultivated under Mediterranean climatic conditions.
A First View on the Competencies and Training Needs of Farmers Working with and Researchers Working on Precision Agriculture Technologies
Authors: Michailidis Anastasios , Charatsari Chrysanthi , Bournaris Thomas , Loizou Efstratios , Paltaki Aikaterini , Lazaridou Dimitra , Lioutas Evagelos D.
Published: January 2024

The penetration of precision agriculture technologies in agrifood systems generates the need for efficient upskilling programs targeted at farmers and other actors. A critical first step in this direction is to uncover the training needs of the actors involved in precision agriculture ecosystems. The present study aimed to identify and assess gaps in competencies related to precision agriculture technologies of Greek livestock farmers and researchers specialized in this field. For farmers, we followed a partially mixed research design. To uncover researchers’ training needs, we chose a qualitative-dominant mixed approach. The results revealed that farmers lack competencies concerning the exploitation of precision agriculture technologies. Depending on their area of expertise, researchers have needs associated with predicting how research affects the future of farming and understanding how precision agriculture artifacts interplay with socio-environmental and economic factors. Despite the limited generalizability of the findings, which represent a limitation associated with the reliance of data on two small sample sizes, our results indicate that, beyond technology-related competencies, it is essential to enhance the capacity of producers and researchers to foresight and shape potential (digital) futures.
National Labelling System of Organic Agriculture and Food Products—How Familiar Are Czech Consumers with the National Organic Agri-Food Brand?
Authors: Zámková Martina , Rojík Stanislav , Prokop Martin , Činčalová Simona , Stolín Radek
Published: January 2024

The organic market in the Czech Republic has been growing dynamically, mainly in the last two decades. It is increasing the number of organic farms and producers of organic food. It was also identified as growing in popularity with Czech customers in the period before the COVID-19 pandemic. In the Czech Republic, organic products are labelled with a national brand called “bio zebra” under direct control of the Czech Ministry of Agriculture. The dependence of the recognition of this brand on selected socio-demographic indicators (gender, age, education, net monthly household income, number of household members, number of children in the family, size of the municipality) was evaluated in a sample of 1197 respondents in total using logistic regression, and the course of partial dependence on individual sociodemographic indicators was monitored in more detail using contingency table analysis. Significant relationships were found between the recognition of this agri-food brand and gender, education, net monthly household income, number of members and children in the family, and size of the municipality where the consumers live. The proportion of respondents who know the label was higher among women and increased with education level, net monthly household income, number of household members and municipality size.
Dependence of the Pea Grain Yield on Climatic Factors under Semi-Arid Conditions
Authors: Gudko Vasiliy , Usatov Alexander , Minkina Tatiana , Duplii Nadezhda , Azarin Kirill , Tatarinova Tatiana V. , Sushkova Svetlana , Garg Ankit , Denisenko Yuri
Published: January 2024

Field peas are one of the most common crops and are grown in various climatic zones. However, the productivity of this crop can be largely limited by climatic factors. This study investigated the influence of climatic factors on pea grain yield in the semi-arid conditions of the Rostov region of Russia in 2008–2020. To quantify climatic factors, agro-climatic variables were used, such as total temperatures below the minimum temperature, the number of days with temperatures below the minimum temperature, total temperatures above the critical temperature, the number of days with temperatures above the critical temperature, and the Selyaninov hydrothermal coefficient. Agro-climatic variables were calculated using daily climatic variables, such as maximum and minimum temperatures, relative air humidity, and precipitation during pea growing season (April–June). The yield of the pea varied from 90 to 250 kg/ha. In general, the productivity of peas is negatively affected by high temperatures and low humidification level. The yield is negatively correlated with accumulative temperatures above the critical temperature and the number of days with temperatures above the critical temperature and positively correlated with the Selyaninov hydrothermal coefficient and the precipitation in all analyzed areas. The influence of the accumulative temperatures above the critical temperature is the most significant. It explains between 6.6% and 78.9% of the interannual variability of the pea yield. The increase in accumulative temperatures above the critical threshold by every 1 °C will contribute to a decrease in pea grain yield by an average of 0.150 kg/ha. The maximum temperatures in May and June (the period of flowering–grain filling) have the most negative impact on the yield. A 1 °C increase in the average maximum temperature during this period will contribute to a decrease in pea yield by an average of 19.175 kg/ha. The influence of total precipitation during the growing season explains between 12.3% and 50.0% of the variability. The 1 mm decrease in the total precipitation for the growing season will lead to a decrease in pea yields by an average of 0.736 kg/ha. The results of this study can be applied to regional yield forecasting, as well as predicting the impact of climate variability on the grain yield of pea crops in arid areas.
Association between Eosinophil Count and Cortisol Concentrations in Equids Admitted in the Emergency Unit with Abdominal Pain
Authors: Villalba-Orero María , Contreras-Aguilar María Dolores , Cerón Jose Joaquín , Fuentes-Romero Beatriz , Valero-González Marta , Martín-Cuervo María
Published: January 2024

Stress leukogram includes eosinopenia as one of its main markers (neutrophilia, eosinopenia, lymphopenia, and mild monocytosis). Cortisol is the main stress biomarker, which is also strongly correlated with the severity of gastrointestinal diseases. This study aimed to determine the relationship between salivary cortisol and the eosinophil cell count (EC) in equids with abdominal pain. To do this, 39 horses with abdominal pain referred to an emergency service were included. All samples were taken on admission, and several parameters and clinical data were included. Equids were classified according to the outcome as survivors and non-survivors. Non-surviving equids presented higher salivary cortisol concentrations (Non-Survivors: 1.580 ± 0.816 µg/dL; Survivors 0.988 ± 0.653 µg/dL; p < 0.05) and lower EC (Non-Survivors: 0.0000 × 103/µL (0.000/0.0075); Survivors: 0.0450 × 103/µL (0.010/0.1825); p < 0.01). In addition, the relationship between salivary cortisol concentration, EC, and the WBC was determined. Only a strong correlation (negative) was observed between cortisol and EC (r = −0.523, p < 0.01). Since cortisol is not an analyte that can be measured routinely in clinical settings such as emergencies, the EC could be a good alternative. While the results are promising, further studies are needed before EC can be used confidently in routine practice to predict survival in cases of abdominal pain.
The Effects of Composite Alkali-Stored Spent Hypsizygus marmoreus Substrate on Carcass Quality, Rumen Fermentation, and Rumen Microbial Diversity in Goats
Authors: Qiu Shuiling , Li Keyao , He Xiangbo , Gu Mingming , Jiang Xinghui , Lu Jianing , Ma Zhiyi , Liang Xuewu , Gan Qianfu
Published: January 2024

The objective of this study was to investigate the effects of composite alkali-stored spent Hypsizygus marmoreus substrate (SHMS) on carcass quality, rumen fermentation, and rumen microbial diversity in goats. Twenty-four 6-month-old Chuanzhong black goats with similar body weights (20 ± 5 kg) were selected and randomly divided into four groups (n = 6 per group) and received four treatments: 0% (control group, CG); 20% (low-addition group, LG); 30% (moderate-addition group, MG); and 40% (high-addition group, HG) of SHMS-replaced silage corn and oat hay. The experiment lasted for 74 days (including a 14 d adaptation period and a 60 d treatment period). The results of this study showed that MG and HG significantly improved the marble score of goat meat (p < 0.05). The flesh color score significantly increased in each group (p < 0.05). The fat color scores significantly increased in LG and MG (p < 0.05). There were no significant effects on the pH value or shear force of the longissimus dorsi in each group (p > 0.05). The cooking loss in MG was higher than that in CG (p < 0.05). The histidine and tyrosine contents in each group of muscles significantly increased (p < 0.05), with no significant effect on fatty acids (p > 0.05). The rumen pH of MG significantly decreased (p < 0.05), while the total volatile fatty acids (TVFAs) and ammoniacal nitrogen (NH3-N) increased by 44.63% and 54.50%, respectively. The addition of the SHMS altered both the alpha and beta diversities of the rumen microbiota and significant differences in the composition and structure of the four microbial communities. The dominant bacterial phylum in each group were Firmicutes and Bacteroidetes, with Prevotella 1 as the dominant bacterial genus. Correlation analysis revealed that rumen bacteria are closely related to the animal carcass quality and rumen fermentation. In the PICRUSt prediction, 21 significantly different pathways were found, and the correlation network showed a positive correlation between the Prevotella 1 and 7 metabolic pathways, while the C5-branched dibasic acid metabolism was positively correlated with nine bacteria. In summary, feeding goats with an SHMS diet can improve the carcass quality, promote rumen fermentation, and alter the microbial structure. The research results can provide a scientific reference for the utilization of SHMS as feed in the goat industry.
Integrated 4D Analysis of Intramuscular Fat Deposition: Quantitative Proteomic and Transcriptomic Studies in Wannanhua Pig Longissimus Dorsi Muscle
Authors: Li Xiaojin , Xie Fei , Li Ruidong , Li Lei , Ren Man , Jin Mengmeng , Zhou Ju , Wang Chonglong , Li Shenghe
Published: January 2024

Wannanhua (WH) is a pig breed indigenous to Anhui Province, China. This breed has a high intramuscular fat (IMF) content, making it an ideal model for investigating lipid deposition mechanisms in pigs. IMF content is one of the main indicators of meat quality in pigs and is regulated by multiple genes and metabolic pathways. Building upon our prior transcriptomic investigation, the present study focused on the longissimus dorsi muscle tissue of Wannanhua (WH) pigs in the rapid fat-deposition stages (120 and 240 days of age). Employing 4D label-free quantitative proteomic analysis, we identified 106 differentially expressed proteins (DEPs). Parallel reaction monitoring (PRM) technology was used to verify the DEPs, and the results showed that the 4D label-free results were reliable and valid. Functional enrichment and protein–protein interaction analyses showed that the DEPs were mainly involved in the skeletal-muscle-associated structural proteins, mitochondria, energy metabolism, and fatty acid metabolism. By integrating transcriptomic data, we identified seven candidate genes including ACADL, ACADM, ANKRD2, MYOZ2, TNNI1, UCHL1, and ART3 that play a regulatory role in fat deposition and muscle development. These findings establish a theoretical foundation for future analyses of lipid deposition traits, contributing to potential enhancements in pig meat quality during breeding and advancing the selection process for Chinese indigenous breeds.
Over 300 km Dispersion of Wild Boar during Hot Summer, from Central Poland to Ukraine
Authors: Popczyk Bartłomiej , Klich Daniel , Nasiadka Paweł , Nieszała Angelika , Gadkowski Krzysztof , Sobczuk Maria , Balcerak Marek , Kociuba Piotr , Olech Wanda , Purski Ludwik
Published: January 2024

The movement of wild boars is a complex process influenced by both internal conditions and external factors. Despite their typically sedentary lifestyle, dispersion constitutes an integral element of the wild boar’s behavior. This report documents the longest observed wild boar dispersal, involving a collared two-year-old male near Warsaw, Poland. The aim of this study was to present the characteristics of movement during the “nomadic phase”, drawing comparisons with the “sedentary phase”. The other aim was to evaluate the influence of meteorological factors on the minimum daily travel distance of the wild boar. We collected data from two-year-old males. The first exhibited long-distance dispersal and the second only demonstrated local movements. We calculated the minimum daily distance of both wild boars based on collar locations and calculated basic statistics of movement. We used a generalized linear model with a gamma distribution and log link function to assess the potential impact of weather conditions on the minimum daily distance of wild boars. We tested maximum daily temperature, average daily temperature, and the sum of daily precipitation. The wild boar during a “nomadic phase” covered a total of 922 km with a mean minimum daily movement of 6 km. The dispersion distance was 307 km. The highest value of the minimum daily distance reached 31.8 km/day. The second wild boar (near Warsaw) covered a mean minimum daily distance of 1.4 km; the highest value of the minimum daily distance was 3.9 km. Both wild boars exhibited no dependence of minimum daily distance on weather conditions. However, when intensive and non-intensive dispersion were analyzed separately, it was demonstrated that the maximum daily temperature positively influenced the minimum daily distance. We speculate that the wild boar was forced to search for water sources after dark on hot days, which induced a longer traveling distance in an unfamiliar environment. This study highlights the significant spatial capabilities of wild boar in the transmission of genes or pathogens. We speculate that extended daily distances during the initial “nomadic phase” might suggest a panicked escape from a perceived threat. It is plausible that the wild boar found improved shelter within tall cereal crops in July and August, which resulted in lower daily distances.
Wicked Problems, Novel Solutions: Nepalese Elephant Tourism and Conservation
Authors: Szydlowski Michelle
Published: January 2024

Endangered Asian elephants (Elephas maximus) find themselves at the center of debates involving politics, land use, human–wildlife conflict, and environmental justice. The intensity of such debates has led scholars to label conservation challenges as wicked problems with profound implications on local and global practice. In elephant range states such as Nepal, these debates are made more complex through human ‘ownership’ of endangered individuals for use in tourism, worship, or co-work. Human perspectives on the ethics of using animals for tourism are changing, even in areas heavily reliant on the tourism industry for survival. These debates become inflamed when non-residents take on adversarial positions despite an acceptance of the ‘ownership’ or ‘use’ of endangered individuals among local communities. Novel approaches are needed if there is any hope of establishing a common ground upon which to build relationships which may benefit community members, international interests, and endangered individuals.
Transcriptome and Weighted Gene Co-Expression Network Analysis for Feather Follicle Density in a Chinese Indigenous Breed
Authors: Wang Jiangxian , Wei Wei , Xing Chaohui , Wang Hao , Liu Meng , Xu Jinmei , He Xinxin , Liu Yanan , Guo Xing , Jiang Runshen
Published: January 2024

Feather follicle density plays an important role in appealing to consumers’ first impressions when making purchasing decisions. However, the molecular network that contributes to this trait remains largely unknown. The aim of this study was to perform transcriptome and weighted gene co-expression network analyses to determine the candidate genes relating to feather follicle density in Wannan male chickens. In total, five hundred one-day-old Wannan male chickens were kept in a conventional cage system. Feather follicle density was recorded for each bird at 12 weeks of age. At 12 weeks, fifteen skin tissue samples were selected for weighted gene co-expression network analysis, of which six skin tissue samples (three birds in the H group and three birds in the L group) were selected for transcriptome analysis. The results showed that, in total, 95 DEGs were identified, and 56 genes were upregulated and 39 genes were downregulated in the high-feather-follicle-density group when compared with the low-feather-follicle-density group. Thirteen co-expression gene modules were identified. The red module was highly significantly negatively correlated with feather follicle density (p < 0.01), with a significant negative correlation coefficient of −0.72. In total, 103 hub genes from the red module were screened. Upon comparing the 103 hub genes with differentially expressed genes (DEGs), it was observed that 13 genes were common to both sets, including MELK, GTSE1, CDK1, HMMR, and CENPE. From the red module, FOXM1, GTSE1, MELK, CDK1, ECT2, and NEK2 were selected as the most important genes. These genes were enriched in the DNA binding pathway, the heterocyclic compound binding pathway, the cell cycle pathway, and the oocyte meiosis pathway. This study suggests that FOXM1, GTSE1, MELK, CDK1, ECT2, and NEK2 may be involved in regulating the development of feather follicle density in Wannan male chickens. The results of this study reveal the genetic structure and molecular regulatory network of feather follicle density in Wannan male chickens, and provide a basis for further elucidating the genetic regulatory mechanism and identifying molecular markers with breeding value.
Validation of a Classification System for Optimal Application of Debridement, Antibiotics, and Implant Retention in Prosthetic Joint Infections following Total Knee Arthroplasty: A Retrospective Review
Authors: Tseng Joyee , Oladipo Victoria , Dandamudi Siddhartha , Jones Conor M. , Levine Brett R.
Published: January 2024

Introduction: Periprosthetic joint infection (PJI) remains a serious complication after total knee arthroplasty (TKA). While debridement, antibiotics, and implant retention (DAIR) are considered for acute PJI, success rates vary. This study aims to assess a new scoring system’s accuracy in predicting DAIR success. Methods: 119 TKA patients (2008–2019) diagnosed with PJI who underwent DAIR were included for analysis. Data were collected on demographics, laboratory values, and clinical outcomes. This was used for validation of the novel classification system consisting of PJI acuteness, microorganism classification, and host health for DAIR indication. Statistical analysis was carried out using SPSS programming. Results: Mean follow-up was 2.5 years with an average age of 65.5 ± 9.1 years, BMI of 31.9 ± 6.2 kg/m2, and CCI of 3.04 ± 1.8. Successful infection eradication occurred in 75.6% of patients. The classification system demonstrated 61.1% sensitivity, 72.4% specificity, and 87.3% positive predictive value (PPV) when the DAIR cutoff was a score less than 6. For a cutoff of less than 8, sensitivity was 100%, specificity was 37.9%, and PPV was 83.3%. Conclusions: To date, no consensus exists on a classification system predicting DAIR success. This novel scoring system, with high PPV, shows promise. Further refinement is essential for enhanced predictive accuracy.
Improving Clostridioides difficile Infectious Disease Treatment Response via Adherence to Clinical Practice Guidelines
Authors: Adukauskienė Dalia , Mickus Rytis , Dambrauskienė Asta , Vanagas Tomas , Adukauskaitė Agnė
Published: January 2024

Clostridioides difficile (C. difficile) is a predominant nosocomial infection, and guidelines for improving diagnosis and treatment were published in 2017. We conducted a single-center, retrospective 10-year cohort study of patients with primary C. difficile infectious disease (CDID) at the largest referral Lithuanian university hospital, aiming to evaluate the clinical and laboratory characteristics of CDID and their association with the outcomes, as well as implication of concordance with current Clinical Practice Guidelines. The study enrolled a total of 370 patients. Cases with non-concordant CDID treatment resulted in more CDID-related Intensive Care Unit (ICU) admissions (7.5 vs. 1.8%) and higher CDID-related mortality (13.0 vs. 1.8%) as well as 30-day all-cause mortality (61.0 vs. 36.1%) and a lower 30-day survival compared with CDID cases with concordant treatment (p < 0.05). Among cases defined by two criteria for severe CDID, only patients with non-concordant metronidazole treatment had refractory CDID (68.8 vs. 0.0%) compared with concordant vancomycin treatment. In the presence of non-concordant metronidazole treatment for severe CDID, only cases defined by two severity criteria had more CDID-related ICU admissions (18.8 vs. 0.0%) and higher CDID-related mortality (25.0 vs. 2.0%, p < 0.05) compared with cases defined by one criterion. Severe comorbidities and the continuation of concomitant antibiotics administered at CDID onset reduced (p < 0.05) the 30-day survival and increased (p = 0.053) 30-day all-cause mortality, with 57.6 vs. 10.7% and 52.0 vs. 25.0%, respectively. Conclusions: CDID treatment non-concordant with the guidelines was associated with various adverse outcomes. In CDID with leukocytes ≥ 15 × 109/L and serum creatinine level > 133 µmol/L (>1.5 mg/dL), enteral vancomycin should be used to avoid refractory response, as metronidazole use was associated with CDID-related ICU admission and CDID-related mortality. Severe comorbidities worsened the outcomes as they were associated with reduced 30-day survival. The continuation of concomitant antibiotic therapy increased 30-day all-cause mortality; thus, it needs to be reasonably justified, deescalated or stopped.
Community Antibiotic Consumption in Cyprus for the Period 2015 to 2022
Authors: Mitsoura Eirini , Kopsidas Ioannis , Charalambous Pambos , Papazisis Georgios , Raikos Nikolaos , Pana Zoi Dorothea
Published: January 2024

Background: Cyprus currently reports to ESAC-Net the total consumption of antimicrobials, without distinguishing between hospital and community-based antibiotic use. As a result, these data can only provide generalized insights into antimicrobial trends in the country. Aim: This study is a first attempt to retrospectively analyze community antibiotic consumption in Cyprus for the period of 2015 to 2022. Material and Methods: Data on community antimicrobial consumption between 2015 and 2022 were extracted from Pharmatrack’s database. Orally administered dispensed antibiotics were categorized under the J01 group of the WHO Anatomical Therapeutic Chemical (ATC) classification and by the WHO’s AWaRe classification of antibiotics. Antibiotic consumption was calculated in both packages consumed and per 1000 inhabitants, overall, by year of consumption and districts. Results: During the period of 2015–2022, there was variability in the mean outpatient antibiotic consumption per 1000 inhabitants among the five districts in Cyprus. Community consumption increased by 38% throughout the study period. Additionally, a decrease of 3% in the consumption of WHO ‘Access’ antibiotics was observed, accompanied with a concurrent increase of 3% in the ‘Watch’ group. Specifically, in 2022 the WHO ‘Access’ group consumption in the Cypriot community was 48%, significantly lower than the WHO’s goal of 60% and the EU’s goal of 70% for ‘Access’ antibiotic consumption. Conclusions: Antibiotic consumption in the community of Cyprus between 2015 and 2022 demonstrated substantial variability among districts, with higher consumption in less populated areas. There was an increasing trend in community consumption over the years and a decreasing trend in the percentage of ‘Access’ antibiotics prescribed.
Infective Endocarditis by Lactobacillus Species—A Narrative Review
Authors: Ioannou Petros , Ziogou Afroditi , Giannakodimos Ilias , Giannakodimos Alexios , Baliou Stella , Samonis George
Published: January 2024

Bacteria of the genus Lactobacillus are microaerophilic or aerotolerant anaerobic Gram-positive non-spore-forming rods. They are considered essential members of the human gut microbiome; however, recent studies have revealed that these microorganisms are less predominant in the gut microbiome than initially thought. Lactobacillus spp. is mainly known for its use as a probiotic in foods and supplements to prevent and treat specific issues such as infectious diseases, irritable bowel syndrome, and diabetes mellitus. However, Lactobacillus spp. may occasionally cause infections such as bacteremia or infective endocarditis (IE). The present study aimed to review all cases of IE by Lactobacillus spp. and describe the epidemiology, microbiology, clinical characteristics, treatment, and outcomes of this infection by collecting relevant data from studies existing in Pubmed and Scopus until 28 September 2023. A total of 77 studies containing data for 82 patients were included. The median age was 56 years, and 69.6% were male. A prosthetic valve was present in 16% of patients, and 17.3% had previously been on probiotics. The aortic valve was the most commonly involved intracardiac site, followed by the mitral valve. Fever, embolic phenomena, sepsis, and heart failure were the most common clinical presentations. Aminoglycosides and penicillin were the most commonly used antimicrobials for definitive treatment. Surgery was performed in 53.7% of patients. Overall mortality was 17.1%. IE in prosthetic valves and presentation with shock were independently associated with overall mortality.
Finsformer: A Novel Approach to Detecting Financial Attacks Using Transformer and Cluster-Attention
Authors: An Hao , Ma Ruotong , Yan Yuhan , Chen Tailai , Zhao Yuchen , Li Pan , Li Jifeng , Wang Xinyue , Fan Dongchen , Lv Chunli
Published: January 2024

This paper aims to address the increasingly severe security threats in financial systems by proposing a novel financial attack detection model, Finsformer. This model integrates the advanced Transformer architecture with the innovative cluster-attention mechanism, dedicated to enhancing the accuracy of financial attack behavior detection to counter complex and varied attack strategies. A key innovation of the Finsformer model lies in its effective capture of key information and patterns within financial transaction data. Comparative experiments with traditional deep learning models such as RNN, LSTM, Transformer, and BERT have demonstrated that Finsformer excels in key metrics such as precision, recall, and accuracy, achieving scores of 0.97, 0.94, and 0.95, respectively. Moreover, ablation studies on different feature extractors further confirm the effectiveness of the Transformer feature extractor in processing complex financial data. Additionally, it was found that the model’s performance heavily depends on the quality and scale of data and may face challenges in computational resources and efficiency in practical applications. Future research will focus on optimizing the Finsformer model, including enhancing computational efficiency, expanding application scenarios, and exploring its application on larger and more diversified datasets.
Quantum Key Distribution with Post-Processing Driven by Physical Unclonable Functions
Authors: Nikolopoulos Georgios M. , Fischlin Marc
Published: January 2024

Quantum key distribution protocols allow two honest distant parties to establish a common truly random secret key in the presence of powerful adversaries, provided that the two users share a short secret key beforehand. This pre-shared secret key is used mainly for authentication purposes in the post-processing of classical data that have been obtained during the quantum communication stage, and it prevents a man-in-the-middle attack. The necessity of a pre-shared key is usually considered to be the main drawback of quantum key distribution protocols, and it becomes even stronger for large networks involving more than two users. Here, we discuss the conditions under which physical unclonable functions can be integrated in currently available quantum key distribution systems in order to facilitate the generation and the distribution of the necessary pre-shared key with the smallest possible cost in the security of the systems. Moreover, the integration of physical unclonable functions in quantum key distribution networks allows for real-time authentication of the devices that are connected to the network.
Dialogue between the Concept of the Object in the Theater of Tadeusz Kantor and the Theatrical Praxis of the Periférico de Objetos
Authors: Cytlak Katarzyna
Published: January 2024

Tadeusz Kantor was a Polish artist and theater director who directly influenced the conceptual understanding of theater, especially in Argentina following two visits to Buenos Aires with his troupe Cricot 2 in the 1980s. He exerted a particularly strong influence on the Periférico de Objetos [The Periphery of Objects], a troupe founded in Buenos Aires in 1989 by Daniel Veronese, Ana Alvarado and Emilio García Wehbi, labelled by critics as “the Argentine theatre of the image”. Despite radically different socio-cultural contexts, elements arising from Kantor’s theater practices (especially his idea of the “poor object” and his concept of “reality of the lowest rank”) acquired distinctly different meanings in Latin America from those coined by Kantor. A nuanced examination of the Periférico de Objetos indicates that Kantor’s concepts, which in their original context resisted politicization, played an important role in the creation of a socially and politically engaged theatre. His concepts, adapted to local realities by the Periférico de Objetos, were reflected in debates surrounding the recent Argentinian past, most notably, the post-dictatorship period.
Effect of Topical Programmed Death-Ligand1 on Corneal Epithelium in Dry Eye Mouse
Authors: Lee Ko Eun , Oh Seheon , Bhujel Basanta , Kim Chang Min , Lee Hun , Park Jin Hyoung , Kim Jae Yong
Published: January 2024

Dry eye disease (DED) is a growing health concern that impacts millions of individuals every year, and is associated with corneal injury, excessive oxidative stress and inflammation. Current therapeutic strategies, including artificial tears and anti-inflammatory agents, are unable to achieve a permanent clinical cure due to their temporary nature or adverse side effects. Therefore, here, we investigated the effectiveness of the topical administration of programmed death-ligand 1 (PD-L1) in the mouse model of DED. The model was generated in C57BL/6 mice by excising the extra orbital lacrimal gland and causing desiccation stress with scopolamine injections. Subsequently, either phosphate-buffered saline (3 µL/eye) or PD-L1 (0.5 µg/mL) was topically administered for 10 days. Tear volume was evaluated with phenol red thread, and corneal fluorescein staining was observed to quantify the corneal epithelial defect. Corneas were collected for histological analysis, and the expression levels of inflammatory signaling proteins such as CD4, CD3e, IL-17, IL-1β, pIkB-α, pNF-kB and pERK1/2 were assessed through immunofluorescence and Western blot techniques. Our results demonstrate that desiccating stress-induced corneal epithelial defect and tear secretion were significantly improved by topical PD-L1 and could reduce corneal CD4+ T cell infiltration, inflammation and apoptosis in a DED mouse model by downregulating IL-17 production and ERK1/2-NFkB pathways.
The Plasma Neurofilament Light Chain, Brain-Derived Neurotrophic Factor, and Risk of Depression in Chronic Hemodialysis Patients
Authors: Stanisławska Martyna , Roman Maja , Nowicki Michał
Published: January 2024

Introduction: Depression is highly prevalent among hemodialysis patients. Understanding the relationship between the plasma neurofilament light chain (NfL) and brain-derived neurotrophic factor (BDNF) may help us to better understand the mechanisms of depression. This study determined their impact, alongside that of other factors, on the risk of depression in hemodialysis patients. Methods: The study enrolled 82 patients undergoing chronic hemodialysis. Serum NfL, BDNF, uric acid, urea, potassium, calcium, phosphorus, intact parathyroid hormone, and C-reactive protein (CRP) levels were measured. The patients completed the Beck Depression Inventory (BDI). Blood pressure values, body mass before and after hemodialysis, and weekly duration of hemodialysis in hours were assessed. For 19-month survival analysis, the patients were stratified according to baseline BDI scores. Results: Based on the BDI score, 18.3% of the patients had an increased risk of depression. Lower scores were associated with significantly longer duration of hemodialysis treatment (37.5 (25–57) 24 (14–37) months, p = 0.01). Within the 19-month survival analysis, 31.7% of patients died. The patients with BDI scores above the median had significantly lower survival than those below the median (log-rank test p = 0.02). No significant differences in serum BDNF levels (192.7 [125.2–278.2]; 207.7 [142.8–265.8] pg/mL, p = 0.40), or NfL concentrations (1431.5 [1182.6–1625.7]; 1494.6 [1335.7–1667] kDa, p = 0.52) were found between patients with lower and higher risk of depression. Patients with BDI scores above the median had significantly higher levels of CRP (9.6 [4.4–14]) than those with scores below the median (3.6 [2.2–7.5], p = 0.01). A significant positive correlation was found between the BDI score and serum CRP level (r = 0.38, p = 0.01). A significant negative correlation was observed between the BDI score and URR% value (r = −0.36, p = 0.02). Conclusions: Patients with lower BDI scores had a longer dialysis duration, indicating a potential negative association between depression risk and length of dialysis treatment. Neither serum NfL nor BDNF levels can serve as markers of depression risk in the dialysis population.
Left Ventricular-Arterial Coupling as an Independent Predictor of Adverse Events in Young Patients with ST Elevation Myocardial Infarction—A 3D Echocardiographic Study
Authors: Scarlatescu Alina Ioana , Micheu Miruna Mihaela , Petre Ioana Gabriela , Oprescu Nicoleta , Mihail Ana Maria , Cojocaru Ioana Denise , Vatasescu Radu Gabriel
Published: January 2024

Left ventricular-arterial coupling (VAC) is a key determinant of global cardiovascular performance, calculated as the ratio between arterial elastance (EA) and left ventricular end-systolic elastance (EES). Over the years, acute myocardial infarction (STEMI) has remained an important cause of morbidity and mortality worldwide. Although, until recently, it was considered a disease occurring mostly in older patients, its prevalence in the young population is continuously rising. In this study, we aimed to investigate the role of 3D VAC and its derived indices in predicting adverse outcomes in young patients with STEMI. We prospectively enrolled 84 young patients (18–51 years) with STEMI who underwent primary PCI and 28 healthy age and sex-matched controls. A 3D echocardiography was used for non-invasive measurements of end-systolic elastance (EES), arterial elastance (EA), and VAC (EA/EES). The occurrence of major adverse cardiac events (MACE) was assessed one year after the index STEMI. Out of 84 patients, 15.4% had adverse events at 12 months follow-up. Patients were divided into two groups according to the presence or absence of MACE. There were no significant differences in arterial elastance between the two groups. EA was higher in the MACE group but without statistical significance (2.65 vs. 2.33; p = 0.09). EES was significantly lower in the MACE group (1.25 ± 0.34 vs. 1.91 ± 0.56. p < 0.0001) and VAC was higher (2.2 ± 0.62 vs. 1.24 ± 0.29, p < 0.0001). ROC analysis showed that VAC has a better predictive value for MACE (AUC 0.927) compared with EA or EEA but also compared with a classical determinant of LV function (LVEF and LVGLS). A VAC value over 1.71 predicts unfavourable outcome with 83.3% sensitivity and 97.1% specificity. In both univariate and multivariate COX regression analysis, VAC remained an independent predictor for MACE and demonstrated incremental prognostic value over LVEF and LVGLS in the proposed statistical models. In conclusion, 3D VAC is an independent predictor of adverse events in young patients with STEMI at a 12 month follow-ups and could be used for a more accurate risk stratification in the acute phase.