bluuebunny/crossref_metadata_2025 huggingface dataset:
Dynamic Analysis of Ball Screw Feed System with the Effects of Excitation Amplitude and Design Parameters
Authors: Liu Chang , Zhao Chunyu , Liu Zhendong , Wang Shuai
Published: July 2021

In this paper, a nine degree-of-freedom dynamic model of the ball screw feed system considering the contact nonlinearity between balls and raceways is established to analyze the vibration characteristics. The position relationship between raceway centers for the ball screw and bearings is determined by using the homogeneous coordinate transformation, and then the restoring force functions along the axial and lateral directions are derived. The dynamic equations of the feed system are solved by using Newmark method, and the proposed model is verified by the experimental method. Furthermore, the effect of the excitation amplitude on the axial vibration of the feed system is investigated by the frequency-amplitude curve and 3-D frequency spectrum. With the increase of excitation amplitude, the dynamic response of the feed system exits the softening, hardening type nonlinearity and jump phenomenon. Additionally, the effects of the initial contact angle, length of screw shaft and number of loaded balls on the axial vibration of the feed system in the resonance region are discussed. The results show that the dynamic model established in this paper is suitable for improving the machining accuracy and stability of the ball screw feed system.
Insights on the Adaptation of Foeniculum vulgare Mill to Iron Deficiency
Authors: Wasli Hanen , Jelali Nahida , Saada Mariem , Ksouri Riadh , Cardoso Susana M.
Published: July 2021

Iron (Fe) deficiency causes great disturbances to plant growth, productivity and metabolism. This study investigated the effect of bicarbonate-induced Fe deficiency on Foeniculum vulgare (Mill) growth, nutrient uptake, the accumulation of secondary metabolites and the impact on bioactivities. When grown under indirect Fe deficiency conditions (+Fe +Bic), the plants decreased their total mass, an effect that was clearly evident in shoots (−28%). Instead, roots were the main organ affected regarding variations in the phenolic profile and their respective functionalities. Hydromethanolic extracts from bicarbonate-treated roots had a remarkable increase in the levels of phenolic compounds, both of flavonoids (isoquercetin and isorhamnetin) and phenolic acids (gallic acid, chlorogenic acid, syringic acid, ferulic acid, caffeic acid and trans-cinnamic acid), when compared to equivalent extracts from control plants. In addition, they exhibited higher scavenging abilities of DPPH•, NO•, RO2•, as well as inhibitory capacities towards the activity of lipoxygenase (LOX), xanthine oxidase (XO) and acetylcholinesterase (AChE). The overall results suggest that fennel species may modulate secondary metabolites metabolism to fight damages caused by iron deficiency.
Biochemical Composition of Eggplant Fruits: A Review
Authors: Sharma Meenakshi , Kaushik Prashant
Published: July 2021

Eggplant is one of the most important vegetable crops known for its nutritive benefits due to the abundance of various bioactive compounds, which include proteins, vitamins, minerals, carbohydrates, phenolics, and dry matter content. In addition, eggplant has significant pharmaceutical properties that have been recently recognized. Eggplant produces secondary metabolites, including glycoalkaloids, antioxidant compounds, and vitamins, which appear to be the major source of its health benefits. It has been reported that there is a considerable correlation between the regular use of phytochemicals and the defense against diseases. Therefore, researchers must analyze the biochemical composition of eggplants to obtain more information about their nutritional quality and health benefits. In this review, an attempt is made to explain the qualitative and quantitative aspects of different biochemicals present in eggplant, in addition to their beneficial health effects.
TikTok as a New Player in the Contemporary Arts Market: A Study with Special Consideration of Feminist Artists and a New Generation of Art Collectors
Authors: Gerlieb Anne
Published: July 2021

How do social-media platforms such as TikTok function as a neutralising factor in the gatekeeping process in times of COVID-19 restrictions? How does TikTok change the experience culture in arts, and how does this impact how artists frame their working process alongside primary gatekeepers? During the COVID-19 pandemic, TikTok attracted many artists, who used the platform to take their practice, and thereby their self-marketing, into their own hands. At the same time, a new generation of collectors use TikTok to discover art under popular hashtag #feministartists. When artists label their work with #feministartists, they insert themselves into the gatekeeping process, and use opportunities and restrictions bounded to that specific hashtag. The study examines this process of professional self-positioning by using interviews with contemporary artists, curators, and observations on TikTok, artist talks, and secondary interviews with artists on online platforms. The findings suggest a variation in how one trades in or trades on “feminist artist”, accessing resources, and gaining exposure. A focus on “feminist artists” is restrictive for consolidating artists’ efforts to pursue specific professional, social, political, and economic agendas through art.
A CFD Approach for Risk Assessment Based on Airborne Pathogen Transmission
Authors: Motamedi Zoka Hamid , Moshfeghi Mohammad , Bordbar Hadi , Mirzaei Parham A. , Sheikhnejad Yahya
Published: July 2021

The outbreak of COVID-19 necessitates developing reliable tools to derive safety measures, including safe social distance and minimum exposure time under different circumstances. Transient Eulerian–Lagrangian computational fluid dynamics (CFD) models have emerged as a viably fast and economical option. Nonetheless, these CFD models resolve the instantaneous distribution of droplets inside a computational domain, making them incapable of directly being used to assess the risk of infection as it depends on the total accumulated dosage of infecting viruses received by a new host within an exposure time. This study proposes a novel risk assessment model (RAM) to predict the temporal and spatial accumulative concentration of infectious exhaled droplets based on the bio-source’s exhalation profile and droplet distribution using the CFD results of respiratory events in various environmental conditions. Unlike the traditional approach in the bulk movement assessment of droplets’ outreach in a domain, every single droplet is traced inside the domain at each time step, and the total number of droplets passing through any arbitrary position of the domain is determined using a computational code. The performance of RAM is investigated for a series of case studies against various respiratory events where the horizontal and the lateral spread of risky zones are shown to temporarily vary rather than being fixed in space. The sensitivity of risky zones to ambient temperature and relative humidity was also addressed for sample cough and sneeze cases. This implies that the RAM provides crucial information required for defining safety measures such as safety distances or minimum exposure times in different environments.
Sequencing and Chromosome-Scale Assembly of Plant Genomes, Brassica rapa as a Use Case
Authors: Istace Benjamin , Belser Caroline , Falentin Cyril , Labadie Karine , Boideau Franz , Deniot Gwenaëlle , Maillet Loeiz , Cruaud Corinne , Bertrand Laurie , Chèvre Anne-Marie , Wincker Patrick , Rousseau-Gueutin Mathieu , Aury Jean-Marc
Published: July 2021

With the rise of long-read sequencers and long-range technologies, delivering high-quality plant genome assemblies is no longer reserved to large consortia. Not only sequencing techniques, but also computer algorithms have reached a point where the reconstruction of assemblies at the chromosome scale is now feasible at the laboratory scale. Current technologies, in particular long-range technologies, are numerous, and selecting the most promising one for the genome of interest is crucial to obtain optimal results. In this study, we resequenced the genome of the yellow sarson, Brassica rapa cv. Z1, using the Oxford Nanopore PromethION sequencer and assembled the sequenced data using current assemblers. To reconstruct complete chromosomes, we used and compared three long-range scaffolding techniques, optical mapping, Omni-C, and Pore-C sequencing libraries, commercialized by Bionano Genomics, Dovetail Genomics, and Oxford Nanopore Technologies, respectively, or a combination of the three, in order to evaluate the capability of each technology.
Early Inflammatory Cytokine Expression in Cerebrospinal Fluid of Patients with Spontaneous Intraventricular Hemorrhage
Authors: Ziai Wendy C. , Parry-Jones Adrian R. , Thompson Carol B. , Sansing Lauren H. , Mullen Michael T. , Murthy Santosh B. , Mould Andrew , Nekoovaght-Tak Saman , Hanley Daniel F.
Published: July 2021

We investigated cerebrospinal fluid (CSF) expression of inflammatory cytokines and their relationship with spontaneous intracerebral and intraventricular hemorrhage (ICH, IVH) and perihematomal edema (PHE) volumes in patients with acute IVH. Twenty-eight adults with IVH requiring external ventricular drainage for obstructive hydrocephalus had cerebrospinal fluid (CSF) collected for up to 10 days and had levels of interleukin-1α (IL-1α), IL-1β, IL-6, IL-8, IL-10, tumor necrosis factor-α (TNFα), and C-C motif chemokine ligand CCL2 measured using enzyme-linked immunosorbent assay. Median [IQR] ICH and IVH volumes at baseline (T0) were 19.8 [5.8–48.8] and 14.3 [5.3–38] mL respectively. Mean levels of IL-1β, IL-6, IL-10, TNF-α, and CCL2 peaked early compared to day 9–10 (p < 0.05) and decreased across subsequent time periods. Levels of IL-1β, IL-6, IL-8, IL-10, and CCL2 had positive correlations with IVH volume at days 3–8 whereas positive correlations with ICH volume occurred earlier at day 1–2. Significant correlations were found with PHE volume for IL-6, IL-10 and CCL2 at day 1–2 and with relative PHE at days 7–8 or 9–10 for IL-1β, IL-6, IL-8, and IL-10. Time trends of CSF cytokines support experimental data suggesting association of cerebral inflammatory responses with ICH/IVH severity. Pro-inflammatory markers are potential targets for injury reduction.
Anthraquinones as Inhibitors of SOS RAS-GEF Activity
Authors: Fernández-Medarde Alberto , Fuentes-Mateos Rocío , García-Navas Rósula , Juan Andrea Olarte-San , Sánchez-López José María , Fernández-Medarde Antonio , Santos Eugenio
Published: July 2021

Recent breakthroughs have reignited interest in RAS GEFs as direct therapeutic targets. To search for new inhibitors of SOS GEF activity, a repository of known/approved compounds (NIH-NACTS) and a library of new marine compounds (Biomar Microbial Technologies) were screened by means of in vitro RAS-GEF assays using purified, bacterially expressed SOS and RAS constructs. Interestingly, all inhibitors identified in our screenings (two per library) shared related chemical structures belonging to the anthraquinone family of compounds. All our anthraquinone SOS inhibitors were active against the three canonical RAS isoforms when tested in our SOS GEF assays, inhibited RAS activation in mouse embryonic fibroblasts, and were also able to inhibit the growth of different cancer cell lines harboring WT or mutant RAS genes. In contrast to the commercially available anthraquinone inhibitors, our new marine anthraquinone inhibitors did not show in vivo cardiotoxicity, thus providing a lead for future discovery of stronger, clinically useful anthraquinone SOS GEF blockers.
Recognition of Oxidized Lipids by Macrophages and Its Role in Atherosclerosis Development
Authors: Mushenkova Nataliya V. , Bezsonov Evgeny E. , Orekhova Varvara A. , Popkova Tatyana V. , Starodubova Antonina V. , Orekhov Alexander N.
Published: July 2021

Atherosclerosis is a multifactorial chronic disease that has a prominent inflammatory component. Currently, atherosclerosis is regarded as an active autoimmune process that involves both innate and adaptive immune pathways. One of the drivers of this process is the presence of modified low-density lipoprotein (LDL). For instance, lipoprotein oxidation leads to the formation of oxidation-specific epitopes (OSE) that can be recognized by the immune cells. Macrophage response to OSEs is recognized as a key trigger for initiation and a stimulator of progression of the inflammatory process in the arteries. At the same time, the role of oxidized LDL components is not limited to pro-inflammatory stimulation, but includes immunoregulatory effects that can have protective functions. It is, therefore, important to better understand the complexity of oxidized LDL effects in atherosclerosis in order to develop new therapeutic approaches to correct the inflammatory and metabolic imbalance associated with this disorder. In this review, we discuss the process of oxidized LDL formation, mechanisms of OSE recognition by macrophages and the role of these processes in atherosclerosis.
Description of Daily Living Skills and Independence: A Cohort from a Multidisciplinary Down Syndrome Clinic
Authors: Krell Kavita , Haugen Kelsey , Torres Amy , Santoro Stephanie
Published: July 2021

Levels of independence vary in individuals with Down syndrome (DS). We began this study to describe the current life skills in our clinic population of children and adults with DS. We collected and reviewed demographics, living situation, and life skills from an electronic intake form used in clinic procedures. Descriptive statistics for this cohort study included mean, standard deviation, and frequencies. From 2014–2020, 350 pediatric and 196 adult patients (range 0–62 years) with a first visit to the Massachusetts General Hospital Down Syndrome Program are described. Pediatric patients were most often enrolled in school, and in an inclusion setting. Adult patients were most often participating in a day program, living with family, and wanted to continue living with family in the future. Most (87%) of adults with DS communicated verbally, though fewer could use written communication (17%). Life skills of greatest importance to adolescents and adults with DS included: learning about healthy foods (35%), preparing meals (34%), providing personal information when needed (35%), and describing symptoms to a doctor (35%). Life skills for patients with DS are varied; those associated with a medical appointment, such as sharing symptoms with the doctor, could improve for greater independence.
Muscle-Derived Cytokines Reduce Growth, Viability and Migratory Activity of Pancreatic Cancer Cells
Authors: Schwappacher Raphaela , Dieterich Walburga , Reljic Dejan , Pilarsky Christian , Mukhopadhyay Debabrata , Chang David K. , Biankin Andrew V. , Siebler Jürgen , Herrmann Hans J. , Neurath Markus F. , Zopf Yurdagül
Published: July 2021

The evidence that regular physical exercise reduces the risk of developing cancer is well described. However, the interaction between physical exercise and cancer is not fully clarified yet. Several myokines released by skeletal muscle appear to have a direct anti-tumour function. There are few data on myokine secretion after exercise in patients with advanced tumours. Pancreatic cancer (PC) is a very aggressive and usually fatal cancer. To investigate the effects of exercise in PC, the blood of advanced-stage PC patients was analysed after 12 weeks of resistance training using whole-body electromyostimulation. After the 12-week training period, the patient serum inhibited the proliferation and the motility of PC cells and enhanced PC cell apoptosis. The impact of exercise training was also investigated in an exercise-mimicking in vitro model using electric pulse stimulation of human myotubes and revealed similar anti-tumour effects on PC cells, clearly indicating direct cancer-protective properties of activated skeletal muscle. Protein and gene expression analyses in plasma from exercise-trained patients and in myotube cultures after in vitro exercise showed that interleukin 10 (IL10), C-X-C motif ligand 1 (CXCL1) and C-C motif chemokine ligand 4 (CCL4) are myokines released from activated skeletal muscle. In accordance with the effects of serum from exercise-trained patients, the supplementation with recombinant IL10, CXCL1 and CCL4 impaired growth and migration of PC cells. Treatment of PC cells with these myokines upregulated caspase 3/7 expression and the cleavage of poly(ADP-ribose) polymerase, leading to enhanced PC cell death. The identification of myokines with anti-tumour properties in advanced-stage PC patients after exercise opens a new perspective in supportive therapy with sports and exercise for cancer patients.
Expression of HLA-DR in Cytotoxic T Lymphocytes: A Validated Predictive Biomarker and a Potential Therapeutic Strategy in Breast Cancer
Authors: Saraiva Diana P. , Azeredo-Lopes Sofia , Antunes Ana , Salvador Rute , Borralho Paula , Assis Beatriz , Pereira Isabel L. , Seabra Zita , Negreiros Ida , Jacinto António , Braga Sofia , Cabral M. Guadalupe
Published: July 2021

Neoadjuvant chemotherapy (NACT) is common in breast cancer (BC) treatment, though more than half of the patients lack an effective response. Therefore, new predictive biomarkers and alternative therapies are crucial. Previously, we proposed HLA-DR-expressing cytotoxic T lymphocytes (CTLs) as a potential biomarker of the response to NACT. To validate this observation and further investigate these cells, 202 BC patients were enrolled. Flow cytometry analyses were performed in 61 biopsies and 41 blood samples pre-NACT and 100 non-NACT tumor samples. All the patients were followed up for 34 months. Blood-isolated immune cells were cultured with BC cell lines in a 3D system. We confirmed that HLA-DR level in CTLs is a highly sensitive, specific, and independent biomarker to predict response to NACT and developed a predictive probability model. This biomarker was also associated with progression-free survival, regardless of the treatment. The clinical observations are substantiated by the anti-tumor properties of HLA-DR-expressing CTLs. Intriguingly, HLA-DR level in CTLs can be modulated ex vivo, boosting their capacity to kill tumor cells synergistically with doxorubicin. Thus, HLA-DR expression in CTLs is a validated tool to select patients that will actually benefit from NACT, and its stimulation might be a novel therapeutic approach for BC.
Association of Mitochondrial DNA Copy Number and Telomere Length with Prevalent and Incident Cancer and Cancer Mortality in Women: A Prospective Swedish Population-Based Study
Authors: Li Yanni , Sundquist Kristina , Wang Xiao , Zhang Naiqi , Hedelius Anna , Sundquist Jan , Memon Ashfaque
Published: July 2021

Changes in mitochondrial DNA copy number (mtDNA-CN) and telomere length have, separately, been proposed as risk factors for various cancer types. However, those results are conflicting. Here, mtDNA-CN and relative telomere length were measured in 3225 middle-aged women included in a large population-based prospective cohort. The baseline mtDNA-CN in patients with prevalent breast cancer was significantly higher (12.39 copies/µL) than cancer-free individuals. During an average of 15.2 years of follow-up, 520 patients were diagnosed with cancer. Lower mtDNA-CN was associated with decreased risk of genital organ cancer (hazard ratio (HR), 0.84), and shorter telomere length was associated with increased risk of urinary system cancer (HR, 1.79). Furthermore, mtDNA-CN was inversely associated with all-cause (HR, 1.20) and cancer-specific mortality (HR, 1.21) when considering all cancer types. Surprisingly, shorter telomere length was associated with decreased risk of cancer-specific mortality when considering all cancer types (HR, 0.85). Finally, lower mtDNA-CN and shorter telomere length were associated with increased risk of both all-cause and cancer-specific mortality in genital organ cancer patients. In this study population, we found that mtDNA-CN and telomere length were significantly associated with prevalent and incident cancer and cancer mortality. However, these associations were cancer type specific and need further investigation.
Early-Phase Interventional Trials in Oral Cancer Prevention
Authors: McCarthy Caroline , Fedele Stefano , Ottensmeier Christian , Shaw Richard J.
Published: July 2021

The increasing breadth of molecular targets, promise of immune-targeted therapies and repurposed agents have heightened interest in cancer prevention. While, to date, testing of oral cancer chemoprevention strategies has failed to deliver therapeutic agents for routine clinical practice, there remains an urgent need for further clinical research to overcome this hurdle. Patients at the greatest risk of disease stand to benefit the most from inclusion in clinical trials; therefore, there is a need to carefully define this population using validated clinical and molecular markers. Safety, tolerability and the efficacy of interventions is assessed through carefully selected endpoints. These endpoints may include pharmacodynamic, clinical, histological and on-target molecular modifications as an individual or as a composite endpoint. Early-phase trials provide an area of opportunity to explore novel and repurposed agents in the setting of oral cancer chemoprevention, eventually leading to phase III trials with clinical endpoints such as transformation and clinical outcome; these studies are large, lengthy and expensive and should be reserved for the most promising of agents. This paper will explore current evidence in oral cancer chemoprevention, drug repurposing, selection of appropriate endpoints for early-phase trials and novel therapeutic angles in oral cancer chemoprevention.
Rapid Spectroscopic Liquid Biopsy for the Universal Detection of Brain Tumours
Authors: Theakstone Ashton G. , Brennan Paul M. , Jenkinson Michael D. , Mills Samantha J. , Syed Khaja , Rinaldi Christopher , Xu Yun , Goodacre Royston , Butler Holly J. , Palmer David S. , Smith Benjamin R. , Baker Matthew J.
Published: July 2021

Background: To support the early detection and diagnosis of brain tumours we have developed a rapid, cost-effective and easy to use spectroscopic liquid biopsy based on the absorbance of infrared radiation. We have previously reported highly sensitive results of our approach which can discriminate patients with a recent brain tumour diagnosis and asymptomatic controls. Other liquid biopsy approaches (e.g., based on tumour genetic material) report a lower classification accuracy for early-stage tumours. In this manuscript we present an investigation into the link between brain tumour volume and liquid biopsy test performance. Methods: In a cohort of 177 patients (90 patients with high-grade glioma (glioblastoma (GBM) or anaplastic astrocytoma), or low-grade glioma (astrocytoma, oligoastrocytoma and oligodendroglioma)) tumour volumes were calculated from magnetic resonance imaging (MRI) investigations and patients were split into two groups depending on MRI parameters (T1 with contrast enhancement or T2/FLAIR (fluid-attenuated inversion recovery)). Using attenuated total reflection (ATR)-Fourier transform infrared (FTIR) spectroscopy coupled with supervised learning methods and machine learning algorithms, 90 tumour patients were stratified against 87 control patients who displayed no symptomatic indications of cancer, and were classified as either glioma or non-glioma. Results: Sensitivities, specificities and balanced accuracies were all greater than 88%, the area under the curve (AUC) was 0.98, and cancer patients with tumour volumes as small as 0.2 cm3 were correctly identified. Conclusions: Our spectroscopic liquid biopsy approach can identify gliomas that are both small and low-grade showing great promise for deployment of this technique for early detection and diagnosis.
Disruption of RING and PHD Domains of TRIM28 Evokes Differentiation in Human iPSCs
Authors: Mazurek Sylwia , Oleksiewicz Urszula , Czerwińska Patrycja , Wróblewska Joanna , Klimczak Marta , Wiznerowicz Maciej
Published: July 2021

TRIM28, a multi-domain protein, is crucial in the development of mouse embryos and the maintenance of embryonic stem cells’ (ESC) self-renewal potential. As the epigenetic factor modulating chromatin structure, TRIM28 regulates the expression of numerous genes and is associated with progression and poor prognosis in many types of cancer. Because of many similarities between highly dedifferentiated cancer cells and normal pluripotent stem cells, we applied human induced pluripotent stem cells (hiPSC) as a model for stemness studies. For the first time in hiPSC, we analyzed the function of individual TRIM28 domains. Here we demonstrate the essential role of a really interesting new gene (RING) domain and plant homeodomain (PHD) in regulating pluripotency maintenance and self-renewal capacity of hiPSC. Our data indicate that mutation within the RING or PHD domain leads to the loss of stem cell phenotypes and downregulation of the FGF signaling. Moreover, impairment of RING or PHD domain results in decreased proliferation and impedes embryoid body formation. In opposition to previous data indicating the impact of phosphorylation on TRIM28 function, our data suggest that TRIM28 phosphorylation does not significantly affect the pluripotency and self-renewal maintenance of hiPSC. Of note, iPSC with disrupted RING and PHD functions display downregulation of genes associated with tumor metastasis, which are considered important targets in cancer treatment. Our data suggest the potential use of RING and PHD domains of TRIM28 as targets in cancer therapy.
Kinetics of Anti-SARS-CoV-2 Antibody Responses 3 Months Post Complete Vaccination with BNT162b2; A Prospective Study in 283 Health Workers
Authors: Terpos Evangelos , Trougakos Ioannis P. , Karalis Vangelis , Ntanasis-Stathopoulos Ioannis , Gumeni Sentiljana , Apostolakou Filia , Sklirou Aimilia D. , Gavriatopoulou Maria , Skourti Stamatia , Kastritis Efstathios , Korompoki Eleni , Papassotiriou Ioannis , Dimopoulos Meletios A.
Published: July 2021

The aim of this study was to investigate the kinetics of neutralizing antibodies (NAbs) and anti-SARS-CoV-2 anti-S-RBD IgGs up to three months after the second vaccination dose with the BNT162b2 mRNA vaccine. NAbs and anti-S-RBD levels were measured on days 1 (before the first vaccine shot), 8, 22 (before the second shot), 36, 50, and three months after the second vaccination (D111) (NCT04743388). 283 health workers were included in this study. NAbs showed a rapid increase from D8 to D36 at a constant rate of about 3% per day and reached a median (SD) of 97.2% (4.7) at D36. From D36 to D50, a slight decrease in NAbs values was detected and it became more prominent between D50 and D111 when the rate of decline was determined at −0.11 per day. The median (SD) NAbs value at D111 was 92.7% (11.8). A similar pattern was also observed for anti-S-RBD antibodies. Anti-S-RBDs showed a steeper increase during D22–D36 and a lower decline rate during D36–D111. Prior COVID-19 infection and younger age were associated with superior antibody responses over time. In conclusion, we found a persistent but declining anti-SARS-CoV-2 humoral immunity at 3 months following full vaccination with BNT162b2 in healthy individuals.
Activation of Neuroprotective Microglia and Astrocytes at the Lesion Site and in the Adjacent Segments Is Crucial for Spontaneous Locomotor Recovery after Spinal Cord Injury
Authors: Kisucká Alexandra , Bimbová Katarína , Bačová Mária , Gálik Ján , Lukáčová Nadežda
Published: July 2021

Microglia and astrocytes play an important role in the regulation of immune responses under various pathological conditions. To detect environmental cues associated with the transformation of reactive microglia (M1) and astrocytes (A1) into their polarization states (anti-inflammatory M2 and A2 phenotypes), we studied time-dependent gene expression in naive and injured spinal cord. The relationship between astrocytes and microglia and their polarization states were studied in a rat model after Th9 compression (40 g/15 min) in acute and subacute stages at the lesion site, and both cranially and caudally. The gene expression of microglia/macrophages and M1 microglia was strongly up-regulated at the lesion site and caudally one week after SCI, and attenuated after two weeks post-SCI. GFAP and S100B, and A1 astrocytes were profoundly expressed predominantly two weeks post-SCI at lesion site and cranially. Gene expression of anti-inflammatory M2a microglia (CD206, CHICHI, IL1rn, Arg-1), M2c microglia (TGF-β, SOCS3, IL4R α) and A2 astrocytes (Tgm1, Ptx3, CD109) was greatly activated at the lesion site one week post-SCI. In addition, we observed positive correlation between neurological outcome and expression of M2a, M2c, and A2 markers. Our findings indicate that the first week post-injury is critical for modulation of reactive microglia/astrocytes into their neuroprotective phenotypes.
Physical Therapy to Prevent Osteopenia in Preterm Infants: A Systematic Review
Authors: Torró-Ferrero Galaad , Fernández-Rego Francisco Javier , Gómez-Conesa Antonia
Published: July 2021

Background: During the last trimester of pregnancy, about 80% of the infant’s calcium is incorporated, and for this reason, preterm infants have less bone mineralization compared to those born at term. The aim of the present systematic review was to identify, evaluate and summarize the studies that deal with the effect of physiotherapy modalities in the prevention and treatment of osteopenia in preterm infants. Methods: A comprehensive search (09/2019–02/2021) using PubMed, Web of Science, SCOPUS, ProQuest, SciELO, Latindex, ScienceDirect, PEDro and ClinicalTrials.gov was carried out. The following data were extracted: The number of participants, characteristics of the participants, design, characteristics of the intervention, outcome measures, time of evaluation and results. A non-quantitative synthesis of the extracted data was performed. The methodological quality and risk of bias were assessed using a PEDro scale and ROB-2 scale, respectively. Results: A total of 16 studies were analyzed, presenting a methodological quality that ranged from 3 to 8 points, and all showed some concerns regarding their risk of bias. Almost all studies (15/16) used passive mobilizations with joint pressure to prevent osteopenia, but they differed in the intensity and frequency of application. Conclusions: A daily exercise program of passive mobilizations with joint pressure, improves bone mineralization in preterm infants admitted to neonatal units.
Dense Matrix Multiplication Algorithms and Performance Evaluation of HPCC in 81 Nodes IBM Power 8 Architecture
Authors: Estévez Ruiz Eduardo Patricio , Chicaiza Giovanny Eduardo Caluña , Patiño Fabian Rodolfo Jiménez , López Lago Joaquín Cayetano , Thirumuruganandham Saravana Prakash
Published: July 2021

Optimizing HPC systems based on performance factors and bottlenecks is essential for designing an HPC infrastructure with the best characteristics and at a reasonable cost. Such insight can only be achieved through a detailed analysis of existing HPC systems and the execution of their workloads. The “Quinde I” is the only and most powerful supercomputer in Ecuador and is currently listed third on the South America. It was built with the IBM Power 8 servers. In this work, we measured its performance using different parameters from High-Performance Computing (HPC) to compare it with theoretical values and values obtained from tests on similar models. To measure its performance, we compiled and ran different benchmarks with the specific optimization flags for Power 8 to get the maximum performance with the current configuration in the hardware installed by the vendor. The inputs of the benchmarks were varied to analyze their impact on the system performance. In addition, we compile and compare the performance of two algorithms for dense matrix multiplication SRUMMA and DGEMM.
Study on the Internal Mechanism of APD Photocurrent Characteristics Caused by the ms Pulsed Infrared Laser Irradiation
Authors: Chen Liang , Wang Di , Jin Guang-Yong , Wei Zhi
Published: July 2021

In this paper, the sampling current characteristics of the external circuit and the internal mechanism of the current generation in APD irradiated by a millisecond pulse laser were studied. The photocurrent of APD irradiated by a millisecond pulse laser with different energy densities was obtained by the sampling resistance of the external circuit. The photocurrent can be divided into a photocurrent stage, conduction stage and recovery stage in the time domain. This is mainly due to the carrier flow in APD, which leads to the lowering of the barrier between the PN junction. The research results of this paper can be extended to the response of the detector to the high-power infrared pulse laser and provide a certain experimental basis for the design of a millisecond pulse infrared laser detection circuit.
Ultra-Fast Laser Fabrication of Alumina Micro-Sample Array and High-Throughput Characterization of Microstructure and Hardness
Authors: Geng Xiao , Tang Jianan , Sheridan Bridget , Sarkar Siddhartha , Tong Jianhua , Xiao Hai , Li Dongsheng , Bordia Rajendra K. , Peng Fei
Published: July 2021

In the light of recent advances in material informatics, there is a great demand for high-throughput approaches of sample fabrication and property characterization. Currently, no high-throughput approach has been demonstrated for the fast sampling of the microstructure and the correlated properties. In this paper, we demonstrate the ultra-fast fabrication of an alumina sample array and the high-throughput hardness characterization of these sample units. The alumina sample array was fabricated using picosecond (PS) laser micromachining and CO2 laser sintering within a short time (i.e., less than a few minutes). After laser sintering, the hardness of these sample units was characterized using micro-indentation, and the microstructure was observed using scanning electron microscopy (SEM). In each sample unit, the microstructure was uniform for the entire top surface and within about 20 µm depth from the top surface. The relative density (RD) and corresponding micro-hardness of the sample units was found to continuously vary over a wide range from 89% RD with 600 kgf/mm2 hardness to 99% RD with 1609 kgf/mm2 hardness. For these laser-sintered samples, the correlation of hardness and relative density of the alumina matched well with the literature reports on sintered alumina obtained using conventional low-throughput furnace sintering experiments.
Dynamic and Photonic Properties of Field-Induced Gratings in Flexoelectric LC Layers
Authors: Palto Serguei P.
Published: July 2021

For LCs with a non-zero flexoelectric coefficient difference (e1–e3) and low dielectric anisotropy, electric fields exceeding certain threshold values result in transitions from the homogeneous planarly aligned state to the spatially periodic one. Field-induced grating is characterized by rotation of the LC director about the alignment axis with the wavevector of the grating oriented perpendicular to the initial alignment direction. The rotation sign is defined by both the electric field vector and the sign of the (e1–e3) difference. The wavenumber characterizing the field-induced periodicity is increased linearly with the applied voltage starting from a threshold value of about π/d, where d is the thickness of the layer. Two sets of properties of the field-induced gratings are studied in this paper using numerical simulations: (i) the dynamics of the grating appearance and relaxation; (ii) the transmittance and reflectance spectra, showing photonic stop bands in the waveguide mode. It is shown that under ideal conditions, the characteristic time of formation for a spatially limited grating is determined by the amplitude of the electric voltage and the size of the grating itself in the direction of the wave vector. For large gratings, this time can be drastically reduced via spatial modulation of the LC anchoring on one of the alignment surfaces. In the last case, the time is defined not by the grating size, but the period of the spatial modulation of the anchoring. The spectral structure of the field-induced stop bands and their use in LC photonics are also discussed.
Smart Monitoring Pad for Prediction of Pressure Ulcers with an Automatically Activated Integrated Electro-Therapy System
Authors: Saleh Zeena Sh. , Al-Neami Auns Qusai , Raad Haider K.
Published: July 2021

Pressure ulcers (PU) are deep scars on the skin that cause pain, infections and severe health complications. Most movement-impaired subjects are vulnerable to PU, leading to permanent and irreversible skin damage. The system proposed in this paper aims to prevent PU formation with the design and implementation of a wirelessly controlled device that predicts PUs before their occurrence and attempts to prevent it using therapeutic feedback. A flexible pad that consists of multiple types of sensors is used, theses sensors continuously and non-invasively monitor ulcer-related vital signs in vulnerable areas, and uses these data to predict PU with a decision-making process. When PU is detected an electrical stimulation (ES) unit is automatically activated. Stimulation prevents PU formation by increasing local blood flow to the simulated area and eliminating the main factor that leads to PU formation. The system successfully monitored and predicted PU; tests were performed on three healthy volunteers and one volunteer with sacral ulcers. Results including readings of blood oxygenation, force, humidity and temperature were recorded as graphs to monitor decay/increase in values more efficiently.
Evaluation of 16S rDNA Heart Tissue PCR as a Complement to Blood Cultures for the Routine Etiological Diagnosis of Infective Endocarditis
Authors: Rodríguez-García Raquel , Rodríguez-Esteban María Ángeles , Fernández-Suárez Jonathan , Morilla Ana , García-Carús Enrique , Telenti Mauricio , Morales Carlos , Albaiceta Guillermo Muñiz , Fernández Javier
Published: July 2021

Identification of the causative pathogen is required to optimize the effective therapy in infective endocarditis (IE). The aim of this study was to assess a 16S rDNA PCR to identify bacteria from heart valve tissues and to evaluate its usefulness as a complement to blood and removed valves cultures. A total of 266 patients diagnosed with IE from January 2015 to December 2019 were evaluated. Results between 16S rDNA PCR from heart valve tissues were compared with microbiological cultures. Blood cultures were positive in 83.5% of patients diagnosed with IE, while 39.6% and 71.8% of the evaluated heart valve samples were positive by culture and 16S rDNA PCR, respectively. For 32 (12%) patients, 16S rDNA tissue PCR provided valuable information supporting the results of blood cultures in the case of bacteria characteristic from the skin microbiota. Additionally, a microorganism was identified by using 16S rDNA PCR in 36% of blood culture-negative cases. The present study reveals that molecular diagnosis using 16S rDNA tissue PCR provides complementary information for the diagnosis of IE, and it should be recommended in surgical endocarditis, especially when blood cultures are negative.
Thyroid Nodule Characterization: How to Assess the Malignancy Risk. Update of the Literature
Authors: Fresilli Daniele , David Emanuele , Pacini Patrizia , Del Gaudio Giovanni , Dolcetti Vincenzo , Lucarelli Giuseppe Tiziano , Di Leo Nicola , Bellini Maria Irene , D'Andrea Vito , Sorrenti Salvatore , Mascagni Domenico , Biffoni Marco , Durante Cosimo , Grani Giorgio , De Vincentis Giuseppe , Cantisani Vito
Published: July 2021

Ultrasound (US) is the first imaging modality for thyroid parenchyma evaluation. In the last decades, the role of ultrasound has been improved with the introduction of new US software, such as contrast-enhanced ultrasound (CEUS) and US-elastography (USE). USE is nowadays recognized as an essential part of the multiparametric ultrasound (MPUS) examination, in particular for the indeterminate thyroid nodule with possible fine-needle aspiration cytology (FNAC) number reduction; even if further and larger studies are needed to validate it. More controversial is the role of CEUS in thyroid evaluation, due to its high variability in sensitivity and specificity. Semi-automatic US systems based on the computer-aided diagnosis (CAD) system are producing interesting results, especially as an aid to less experienced operators. New knowledge on the molecular mechanisms involved in thyroid cancer is allowing practitioners to identify new genomic thyroid markers that could reduce the number of “diagnostic” thyroidectomies. We have therefore drawn up an updated representation of the current evidence in the literature for thyroid nodule multiparametric ultrasound (MPUS) evaluation with particular regard to USE, the US CAD system and CEUS.
Lattice-Based Logarithmic-Size Non-Interactive Deniable Ring Signatures
Authors: Jia Huiwen , Tang Chunming , Zhang Yanhua
Published: July 2021

Deniable ring signature can be regarded as group signature without group manager, in which a singer is capable of singing a message anonymously, but, if necessary, each ring member is allowed to confirm or disavowal its involvement in the signature via an interactive mechanism between the ring member and the verifier. This attractive feature makes the deniable ring signature find many applications in the real world. In this work, we propose an efficient scheme with signature size logarithmic to the cardinality of the ring. From a high level, we adapt Libert et al.’s zero-knowledge argument system (Eurocrypt 2016) to allow the prover to convince the verifier that its witness satisfies an additional condition. Then, using the Fait-Shamir transformation, we get a non-interactive deniable ring signature scheme that satisfies the anonymity, traceability, and non-frameability under the small integer solution assumption in the random oracle model.
Formulation of Time-Fractional Electrodynamics Based on Riemann-Silberstein Vector
Authors: Stefański Tomasz P. , Gulgowski Jacek
Published: July 2021

In this paper, the formulation of time-fractional (TF) electrodynamics is derived based on the Riemann-Silberstein (RS) vector. With the use of this vector and fractional-order derivatives, one can write TF Maxwell’s equations in a compact form, which allows for modelling of energy dissipation and dynamics of electromagnetic systems with memory. Therefore, we formulate TF Maxwell’s equations using the RS vector and analyse their properties from the point of view of classical electrodynamics, i.e., energy and momentum conservation, reciprocity, causality. Afterwards, we derive classical solutions for wave-propagation problems, assuming helical, spherical, and cylindrical symmetries of solutions. The results are supported by numerical simulations and their analysis. Discussion of relations between the TF Schrödinger equation and TF electrodynamics is included as well.
A Stochastic Model for Block Segmentation of Images Based on the Quadtree and the Bayes Code for It
Authors: Nakahara Yuta , Matsushima Toshiyasu
Published: July 2021

In information theory, lossless compression of general data is based on an explicit assumption of a stochastic generative model on target data. However, in lossless image compression, researchers have mainly focused on the coding procedure that outputs the coded sequence from the input image, and the assumption of the stochastic generative model is implicit. In these studies, there is a difficulty in discussing the difference between the expected code length and the entropy of the stochastic generative model. We solve this difficulty for a class of images, in which they have non-stationarity among segments. In this paper, we propose a novel stochastic generative model of images by redefining the implicit stochastic generative model in a previous coding procedure. Our model is based on the quadtree so that it effectively represents the variable block size segmentation of images. Then, we construct the Bayes code optimal for the proposed stochastic generative model. It requires the summation of all possible quadtrees weighted by their posterior. In general, its computational cost increases exponentially for the image size. However, we introduce an efficient algorithm to calculate it in the polynomial order of the image size without loss of optimality. As a result, the derived algorithm has a better average coding rate than that of JBIG.
Individually Switchable InGaN/GaN Nano-LED Arrays as Highly Resolved Illumination Engines
Authors: Kluczyk-Korch Katarzyna , Moreno Sergio , Canals Joan , Diéguez Angel , Gülink Jan , Hartmann Jana , Waag Andreas , Di Carlo Aldo , Auf der Maur Matthias
Published: July 2021

GaN-based light emitting diodes (LEDs) have been shown to effectively operate down to nanoscale dimensions, which allows further downscaling the chip-based LED display technology from micro- to nanoscale. This brings up the question of what resolution limit of the illumination pattern can be obtained. We show two different approaches to achieve individually switchable nano-LED arrays. We evaluated both designs in terms of near-field spot size and optical crosstalk between neighboring pixels by using finite difference time domain (FDTD) simulations. The numerical results were compared with the performance data from a fabricated nano-LED array. The outcome underlines the influence of geometry of the LED array and materials used in contact lines on the final illumination spot size and shape.
RRAM Random Number Generator Based on Train of Pulses
Authors: Yang Binbin , Arumí Daniel , Manich Salvador , Gómez-Pau Álvaro , Rodríguez-Montañés Rosa , González Mireia Bargalló , Campabadal Francesca , Fang Liang
Published: July 2021

In this paper, the modulation of the conductance levels of resistive random access memory (RRAM) devices is used for the generation of random numbers by applying a train of RESET pulses. The influence of the pulse amplitude and width on the device resistance is also analyzed. For each pulse characteristic, the number of pulses required to drive the device to a particular resistance threshold is variable, and it is exploited to extract random numbers. Based on this behavior, a random number generator (RNG) circuit is proposed. To assess the performance of the circuit, the National Institute of Standards and Technology (NIST) randomness tests are applied to evaluate the randomness of the bitstreams obtained. The experimental results show that four random bits are simultaneously obtained, passing all the applied tests without the need for post-processing. The presented method provides a new strategy to generate random numbers based on RRAMs for hardware security applications.
Efficient Holographic Focusing Metasurface
Authors: Gowda Vinay R. , Imani Mohammadreza F. , Sleasman Timothy , Smith David R.
Published: July 2021

We present the design and experimental demonstration of an efficient holographic metasurface aperture that focuses microwaves in the Fresnel zone. The proposed circular structure consists of two stacked plates with their periphery terminated in a conductive layer. Microwaves are injected into the bottom plate, which forms the feed layer, and are coupled to the top holographic metasurface layer via an annular ring. This coupling results in an inward traveling cylindrical wave in the top layer, which serves as the reference wave for a hologram. The radiating elements consist of a slot pair with their orientations designed to couple efficiently with the cylindrical reference wave while maintaining a linearly polarized focused beam. A general condition on the slot pairs radiated power is proposed to ensure low sidelobe level (SLL) and is validated with full-wave simulation. An aperture that is 20 cm in diameter, operates at 20 GHz in the K-band frequency, and forms a diffraction-limited focal spot at a distance of 10 cm is experimentally demonstrated. The proposed near-field focusing metasurface has high antenna efficiency and can find application as a compact source for Fresnel-zone wireless power transfer and remote sensing schemes.