bluuebunny/crossref_metadata_2025 huggingface dataset:
Weed Strategy Considering the Weed Control Effect and Weed Control Uniformity with Microsprinkler Irrigation
Authors: Wang Hui , Shi Wenpeng , Zha Qing , Ling Gang , Wang Wene , Hu Xiaotao
Published: March 2023

Improper herbicide application without proper personnel protection (PPE) can be harmful. Herbicide application with microsprinkler irrigation reduces direct contact with herbicides with the benefits of being highly efficient, decreasing water and herbicide use, and using precise irrigation and concentration control during agricultural production. Therefore, to propose a reasonable strategy for applying microsprinkler irrigation, a laboratory test was conducted to study the water distribution characteristics, and different herbicide concentrations (1.5 g/L, 2.0 g/L, and 3.0 g/L) were used in a field irrigation experiment with polyethylene microsprinkler hoses. Wheat was selected as the test crop, and the effects of the different herbicide concentrations were compared and analyzed based on the weed control effect and weed control uniformity. The results showed that in comparison to other herbicide concentrations, a higher herbicide application concentration (3.0 g/L) did not have a better application effect. Application concentration and duration influenced each other and synergistically affected the application effect. The weed control effects of the herbicide concentrations at 1.5 g/L and 2.0 g/L were similar and had better application effects than those of the other concentrations. When using this approach, the specific herbicide concentration should be determined according to the crop and soil environmental conditions, and the application concentration and duration should be adjusted reasonably.
Nitrogen Significantly Affected N Cycling Functional Gene Abundances Compared with Phosphorus and Drought in an Alpine Meadow
Authors: Li Haiyan , Zhang Jiaqi , Tian Dashuan , Liu Yinghui , Dong Jingyi
Published: March 2023

Human activities and global climate change have greatly increased nitrogen (N) and phosphorus (P) inputs and altered precipitation patterns in alpine meadows. Functional genes are important indicators of microorganisms that drive the nitrogen cycling process; however, the functional gene responses of soil nitrogen cycling to soil N and P availability and drought remain unclear. Separate or combined treatments of nitrogen and phosphorus fertilization and drought were conducted on the Zoige Wetland in the Qinghai-Tibet Plateau, and the abundances of nitrification functional genes AOA amoA and AOB amoA and denitrification functional genes nirS, nirK, and nosZ were measured to explore the response of functional genes to these treatments. Seven treatments, including control (CK), N addition (N), P addition (P), 50% reduction in precipitation (D), N and P addition (NP), N addition with drought (ND), and NP addition with drought (NPD), were investigated. The results indicated that N application significantly increased AOB amoA abundance, while P application and drought had no significant effects on the abundance of functional genes. The combined treatment of N and P addition and drought increased AOB amoA abundance but did not significantly affect AOA amoA abundance, suggesting that AOB amoA was more responsive to soil N and P availability and moisture change than AOA amoA. However, the abundance of denitrification functional genes was not affected by these treatments. Denitrification functional genes were less sensitive to soil N and P availability and moisture change than nitrification functional genes. The integrated effects of N addition, P addition, and drought did not affect the abundance of the above N cycling functional genes. These results indicate that AOB amoA may play a more critical role in the process of ammonia oxidation than AOA amoA in alpine meadows, and the denitrification genes (nirK, nirS, and nosZ) were better than ammoxidation genes (AOA and AOB) at adapting to the soil environmental changes caused by increasing N and P deposition and drought in alpine meadows.
RDE-YOLOv7: An Improved Model Based on YOLOv7 for Better Performance in Detecting Dragon Fruits
Authors: Zhou Jialiang , Zhang Yueyue , Wang Jinpeng
Published: March 2023

There is a great demand for dragon fruit in China and Southeast Asia. Manual picking of dragon fruit requires a lot of labor. It is imperative to study the dragon fruit-picking robot. The visual guidance system is an important part of a picking robot. To realize the automatic picking of dragon fruit, this paper proposes a detection method of dragon fruit based on RDE-YOLOv7 to identify and locate dragon fruit more accurately. RepGhost and decoupled head are introduced into YOLOv7 to better extract features and better predict results. In addition, multiple ECA blocks are introduced into various locations of the network to extract effective information from a large amount of information. The experimental results show that the RDE-YOLOv7 improves the precision, recall, and mean average precision by 5.0%, 2.1%, and 1.6%. The RDE-YOLOv7 also has high accuracy for fruit detection under different lighting conditions and different blur degrees. Using the RDE-YOLOv7, we build a dragon fruit picking system and conduct positioning and picking experiments. The spatial positioning error of the system is only 2.51 mm, 2.43 mm, and 1.84 mm. The picking experiments indicate that the RDE-YOLOv7 can accurately detect dragon fruits, theoretically supporting the development of dragon fruit-picking robots.
In Vitro Degradability and Methane Production from By-Products Fed to Ruminants
Authors: Bizzuti Beatriz Elisa , Pérez-Márquez Simón , van Cleef Flavia de Oliveira Scarpino , Ovani Vagner Silva , Costa Wilian Santos , Lima Paulo Mello Tavares , Louvandini Helder , Abdalla Adibe Luiz
Published: March 2023

The feasibility of unconventional feed for ruminants must be analyzed for appropriate use as sources of energy or protein that contribute to a sustainable production system addressing concerns about climate change, greenhouse gas emissions, and food security. The objective of this study was to evaluate the nutritive value of by-products including bean residue (BR), bean straw (BS), cassava bagasse (CB), corn straw (CS), orange bagasse (OB), peanut shell (PS), sugarcane bagasse (SB), soybean hull (SH), and sugarcane straw (SS). Chemical composition, in vitro degradability, and fermentative parameters were analyzed and subjected to multivariate and principal component analyses. Crude protein content was highly variable among by-products with BR and SH presenting the highest values (p < 0.001). These values did not differ between BR and SH, while the lowest values were observed in OB, PS, SB, SS, CS, and CB. Neutral detergent fiber content was higher in SB, PS, CS, and SS (p < 0.001), while greater in vitro degradability was found for CB, BR, OB, BS, and SH (p < 0.001). However, the in vitro gas production (p < 0.001) and total short-chain fatty acid concentration (p < 0.001) were higher in CB, BR, BS, and OB. Multivariate cluster analysis showed three distinct groups with the most degradable in group 1 followed by group 2. These results demonstrate that the by-products used in this study did not impair in vitro fermentation, and thus have the potential to be used in the diets of ruminants and to improve the sustainability of the livestock production sector.
Implementation of Feed Efficiency in Iranian Holstein Breeding Program
Authors: Nadri Sara , Sadeghi-Sefidmazgi Ali , Zamani Pouya , Ghorbani Gholam Reza , Toghiani Sajjad
Published: March 2023

This study aimed to evaluate the economic impact of improving feed efficiency on breeding objectives for Iranian Holsteins. Production and economic data from seven dairy herds were used to estimate the economic values of different traits, and a meta-analysis was conducted to analyze the genetic relationships between feed efficiency and other traits. Economic weights were calculated for various traits, with mean values per cow and per year across herds estimated at USD 0.34/kg for milk yield, USD 6.93/kg for fat yield, USD 5.53/kg for protein yield, USD −1.68/kg for dry matter intake, USD −1.70/kg for residual feed intake, USD 0.47/month for productive life, and USD −2.71/day for days open. The Iranian selection index was revised to improve feed efficiency, and the feed efficiency sub-index (FE$) introduced by the Holstein Association of the United States of America was adopted to reflect Iran’s economic and production systems. However, there were discrepancies between Iranian and US genetic coefficients in the sub-index, which could be attributed to differences in genetic and phenotypic parameters, as well as the economic value of each trait. More accurate estimates of economic values for each trait in FE$ could be obtained by collecting dry matter intake from Iranian herds and conducting genetic evaluations for residual feed intake.
Edge-YOLO: Lightweight Infrared Object Detection Method Deployed on Edge Devices
Authors: Li Junqing , Ye Jiongyao
Published: March 2023

Existing target detection algorithms for infrared road scenes are often computationally intensive and require large models, which makes them unsuitable for deployment on edge devices. In this paper, we propose a lightweight infrared target detection method, called Edge-YOLO, to address these challenges. Our approach replaces the backbone network of the YOLOv5m model with a lightweight ShuffleBlock and a strip depthwise convolutional attention module. We also applied CAU-Lite as the up-sampling operator and EX-IoU as the bounding box loss function. Our experiments demonstrate that, compared with YOLOv5m, Edge-YOLO is 70.3% less computationally intensive, 71.6% smaller in model size, and 44.4% faster in detection speed, while maintaining the same level of detection accuracy. As a result, our method is better suited for deployment on embedded platforms, making effective infrared target detection in real-world scenarios possible.
Radiation and Temperature of a Tropical Grassland in Summer Times: Experimental Observations
Authors: Lu Yunan , Wang Tianyu , Huang Chan , Qin Yinghong
Published: March 2023

The surface texture of urbanized regions is altered by the replacement of natural vegetated surfaces with hardened pavement surfaces, which have been described as a heat source for the formation of urban heat islands. Grasslands may store rainfall in their roots and leaves for later cooling, but this has received little attention. This study investigated the radiant flux and temperature of a tropical grassland throughout the summer in order to understand the albedo, long-wave radiation, short-wave radiation, and surface temperature of the grassland over 10 days. The grassland had an albedo of 0.13, which did not fluctuate during the day compared to the albedo of other surfaces in metropolitan areas. Even if the local weather changes considerably, this albedo does not alter significantly. The surface temperature and the air temperature above the grassland increase linearly with the upwelling reflectance, incident solar radiation, and upwelling long-wave radiation. These two temperatures do not correspond with downwelling long-wave radiation, which is influenced by cloud cover in the sky. However, the peaks of these temperatures lag behind the incident shortwave radiation and net radiation that reaches the grassland surface. The finding that the thermal properties of grasslands could be harnessed to reduce the heat absorbed by urban surfaces provides valuable insights into the grasslands’ potential to mitigate the impacts of urbanization on temperature.
Decomposing Fast and Slow Responses of Global Cloud Cover to Quadrupled CO2 Forcing in CMIP6 Models
Authors: Zhou Xixun , Zhang Hua , Wang Qiuyan , Xie Bing
Published: March 2023

Cloud changes and their attribution under global warming still remains a challenge in climatic change studies, especially in decomposing the fast and slow cloud responses to anthropogenic forcing. In this study, the responses of global cloud cover to the quadrupled CO2 forcing are investigated quantitatively by decomposing the total response into fast and slow ones using the multi-model data from the Coupled Model Intercomparison Project Phase 6 (CMIP6). During the quasi-equilibrium period after the quadrupling of CO2 forcing, the global mean changes of simulated total cloud cover (TCC) in the total, fast, and slow responses are −2.42%, −0.64%, and −1.78%, respectively. Overall, the slow response dominates the total response in most regions over the globe with similar spatial patterns. TCC decreases at middle and low latitudes but increases at high latitudes in the total and slow responses. Whereas, it mainly decreases in the middle and low latitudes of the southern hemisphere as well as in the middle and high latitudes of the northern hemisphere in the fast response. A change in vertical motion is the major contributor to the cloud cover change at middle and low latitudes, while the decrease in upper atmospheric temperature leads to an increase in high cloud cover at high latitudes. In addition, the anomaly in water vapor convergence/diffusion also contributes to the cloud cover increase/decrease at low latitudes.
Emissions of PAHs, Nitro-PAHs and Quinones (Oxy-PAHs) Associated to PM1.0 and PM2.5 Emitted by a Diesel Engine Fueled with Diesel-Biodiesel-Ethanol Blends
Authors: Paim Joilson Nascimento , Santos Aldenor Gomes , Araujo Rennan G. O. , Nascimento Madson Moreira , De Andrade Jailson Bittencourt , Guarieiro Lilian Lefol Nani
Published: March 2023

Emissions of PAH, nitro-PAHs, and oxy-PAHs from a diesel engine fueled with diesel-biodiesel-ethanol blends need to be controlled and reduced, as they are unregulated emissions harmful to the environment and human health. The objective of this work was to investigate the effect of ethanol concentration on diesel engine emissions when fueled with diesel–biodiesel–ethanol blends. Ethanol was added with biodiesel–diesel blends. Diesel B7 and two ternary blends, B7E3 and B7E10, with 3% and 10% ethanol, were tested and studied in a diesel engine to determine engine performance characteristics and particulate matter emissions and to quantify polycyclic aromatic compounds (PACs) associated with PM1.0 and PM2.5. Under the same engine conditions, 18 PAHs, 27 nitro-PAHs, and 6 quinones (oxy-PAHs) were determined by GC–MS in real samples obtained from the engine. The mean concentrations of PACs found in the B7, B7E3, and B7E10 blends for PM1.0 ranged from 0.1 µg m−3 (coronene) to 118.1 µg m−3 (2-nitrofluorene). The concentrations for PM2.5 ranged from 0.1 µg m−3 (acenaphthylene) to 99.7 µg m−3 (2-nitrofluorene). Potent mutagens benzanthrone (BA) and 3-nitrobenzanthrone (3-NBA) were found at concentrations ranging from 0.10 µg m−3 to 1.9 µg m−3 and 0.3 µg m−3 to 1.6 µg m−3, respectively. Low molecular weight (LMW) PAHs were mainly PACs bounded to the PM1.0 and PM2.5 particles emitted by B7E10. Flow properties were improved by adding 3% and 10% ethanol to biodiesel. B7E3 and B7E10 blends presented low fuel consumption and a reduction in the emission factor (EF) by the engine. B7E10 blending showed a smaller total concentration of ∑PAH (26.8 µg m−3), ∑nitro-PAH (85.4 µg m−3), and ∑oxy-PAH (6.0 µg m−3) associated with PM2.5 particles compared PM1.0.
Multiscale Flood Disaster Risk Assessment in the Lancang-Mekong River Basin: A Focus on Watershed and Community Levels
Authors: Wu Shengnan , Lei Yu
Published: March 2023

Floods are one of the most frequent and costly natural hazards worldwide, causing significant damage to infrastructure, agriculture, and livelihoods. The Lancang-Mekong River is a major river in Southeast Asia, but the basin is prone to flood disasters that may be exacerbated by climate change. Therefore, to better understand disaster risk and tailor disaster risk reduction measures, this study conducted multiscale flood disaster risk assessments at the watershed and community levels using indicator-based and hydrodynamic model-based methods. Both methods adopted open data with the supplement of local survey data. The results of the study showed that the flood risk is generally higher in the lower reach of the river due to high levels of both hazard and vulnerability. However, the community-scale risk assessment revealed that high flood-risk communities exist in low-risk zones, and vice versa, when the flood risk was assessed at the watershed scale. Such phenomena can lead to inadequate community preparedness for flooding or unnecessary allocation of resources for flood mitigation measures. These findings provide valuable insights for the development of disaster risk reduction strategies, policies, and plans based on an understanding of the risks. Furthermore, they offer a basis for prioritizing and targeting resources, particularly in areas with high population density or vulnerable communities.
Physics-Informed Deep Learning for Reconstruction of Spatial Missing Climate Information in the Antarctic
Authors: Yao Ziqiang , Zhang Tao , Wu Li , Wang Xiaoying , Huang Jianqiang
Published: March 2023

Understanding the influence of the Antarctic on the global climate is crucial for the prediction of global warming. However, due to very few observation sites, it is difficult to reconstruct the rational spatial pattern by filling in the missing values from the limited site observations. To tackle this challenge, regional spatial gap-filling methods, such as Kriging and inverse distance weighted (IDW), are regularly used in geoscience. Nevertheless, the reconstructing credibility of these methods is undesirable when the spatial structure has massive missing pieces. Inspired by image inpainting, we propose a novel deep learning method that demonstrates a good effect by embedding the physics-aware initialization of deep learning methods for rapid learning and capturing the spatial dependence for the high-fidelity imputation of missing areas. We create the benchmark dataset that artificially masks the Antarctic region with ratios of 30%, 50% and 70%. The reconstructing monthly mean surface temperature using the deep learning image inpainting method RFR (Recurrent Feature Reasoning) exhibits an average of 63% and 71% improvement of accuracy over Kriging and IDW under different missing rates. With regard to wind speed, there are still 36% and 50% improvements. In particular, the achieved improvement is even better for the larger missing ratio, such as under the 70% missing rate, where the accuracy of RFR is 68% and 74% higher than Kriging and IDW for temperature and also 38% and 46% higher for wind speed. In addition, the PI-RFR (Physics-Informed Recurrent Feature Reasoning) method we proposed is initialized using the spatial pattern data simulated by the numerical climate model instead of the unified average. Compared with RFR, PI-RFR has an average accuracy improvement of 10% for temperature and 9% for wind speed. When applied to reconstruct the spatial pattern based on the Antarctic site observations, where the missing rate is over 90%, the proposed method exhibits more spatial characteristics than Kriging and IDW.
Impact of Water-Based Coating Substitution on VOCs Emission Characteristics for the Surface-Coating Industries and Policy Effectiveness: A Case Study in Jiangsu Province, China
Authors: Xia Sijia , Zhao Qiuyue , Ji Junhong , Wu Rongrong , Chen Lingxiao , Yin Yin , Liu Qian
Published: March 2023

As solvent-based coatings are gradually phasing out in China, the volatile organic compounds (VOCs) emission characteristics of surface-coating industries have changed rapidly. Sector-based field measurements were conducted to build VOCs emission factors and source profiles of surface-coating industries in Jiangsu Province. A VOCs emission inventory was developed, and the projections for 2020 to 2030 were set. It was found that VOCs content in water-based coatings is 50.8% of solvent-based coatings on average. VOCs emission factors of solvent-based coatings ranged from 0.40 to 0.51 kg kg−1, while those of water-based coatings ranged from 0.14 to 0.24 kg kg−1. Compared to solvent-based coatings, the proportion of aromatics emitted from water-based coatings was 44.2% lower, while the proportion of oxygenated VOCs (OVOCs) was 11.6% higher. The results showed that VOCs emissions were about 134 Gg in Jiangsu Province in 2020, of which the solvent-based coating sources contributed 79.6% of the total. Aromatics were the main species contributing 52.9% of VOCs emissions and 85.9% of ozone formation potential (OFP). According to emission prediction results of four scenarios, the emission reduction of implementing low-content VOCs coating substitution is 8.7% higher than that of adopting the best available end-of-pipe treatment measures by 2030.
Analysis of the Outdoor Microclimate and the Effects on Greek Cultural Heritage Using the Heritage Microclimate Risk (HMR) and Predicted Risk of Damage (PRD) Indices: Present and Future Simulations
Authors: Tringa Efstathia , Tolika Konstantia
Published: March 2023

This study aims to assess the impacts of climate change on the cultural heritage of two Greek areas for both the present time and the future. As the climate is constantly evolving, cultural heritage is continuously exposed to the risk of damage and deterioration. Therefore, museum directors and boards as well as the national ministries must be informed so that measures and practices are adapted to the new climatic conditions. Greece is a country with immense cultural wealth, both tangible and intangible, which stretches back four and a half thousand years. As a homeland of important historical and cultural resources, Greece is vulnerable to climate change. Two up-to-date indices were applied to two Greek areas with high cultural value: Thessaloniki and Delphi. In order to evaluate the suitability of the microclimate and to assess the damage risk, the Heritage Microclimate Risk (HMR) index and the Predicted Risk of Damage (PRD) index were used for two variables: temperature and relative humidity. The study was carried out for three different time periods, the period 1980–2000 (hereafter, the reference period) and the future periods 2039–2059 and 2079–2099. For the reference period, data from three different sources were incorporated in the study (observed, reanalysis, and model data). The simulated data were derived from the Regional Climate Model RegCM4 with a fine spatial resolution of 10 × 10 km. In addition, the emission scenario RCP4.5 was used for the model’s future simulations. The present and future ideal outdoor climate conditions for the monuments were also estimated and are presented in this work. Overall, the future estimations revealed that the ideal outdoor temperature conditions will be higher compared to the reference period, meaning that the monuments will have to adapt to new warmer climate conditions. In addition to the new temperature conditions to which the monuments will have to adapt, the study showed that the monuments will be exposed to “moderate-maximum” risk more often in the future.
Complex Physical Properties of an Adaptive, Self-Organizing Biological System
Authors: Prechl József
Published: March 2023

Physical modeling of the functioning of the adaptive immune system, which has been thoroughly characterized on genetic and molecular levels, provides a unique opportunity to define an adaptive, self-organizing biological system in its entirety. This paper describes a configuration space model of immune function, where directed chemical potentials of the system constitute a space of interactions. A mathematical approach is used to define the system that couples the variance of Gaussian distributed interaction energies in its interaction space to the exponentially distributed chemical potentials of its effector molecules to maintain its steady state. The model is validated by identifying the thermodynamic and network variables analogous to the mathematical parameters and by applying the model to the humoral immune system. Overall, this statistical thermodynamics model of adaptive immunity describes how adaptive biological self-organization arises from the maintenance of a scale-free, directed molecular interaction network with fractal topology.
A Systematic Review of Intracranial Complications in Adults with Pott Puffy Tumor over Four Decades
Authors: Sideris Giorgos , Davoutis Efstathia , Panagoulis Evangelos , Maragkoudakis Pavlos , Nikolopoulos Thomas , Delides Alexander
Published: March 2023

The purpose of this study is to investigate the risk factors of intracranial complications in adult patients with Pott Puffy Tumor (PPT). A systematic review was conducted of clinical studies from January 1983 to December 2022 that reported on PPT adult patients. The full-text articles were reviewed for the patients’ ages, sex, cultured organisms, surgical procedures, clinical sequalae, and underlying diseases that may affect the onset of intracranial complications in PPT adult patients. A total of 106 studies were included. Medical data were reviewed for 125 patients (94 males, 31 females). The median age was 45 years. A total of 52% had comorbidities, mostly head trauma (24.5%), sinus/neurosurgical operations (22.4%), immunosuppression conditions (13.3%), diabetes mellitus (9.1%), cocaine use (7.1%), or dental infections (6.1%). A total of 28 cultures revealed Streptococcus (22.4%), 24 contained staphylococci (19.2%), and 22 cultures contained other pathogens (17.6%). An amount of 30.4% developed intracranial complications, with the most common being epidural abscesses or empyemas (55.3%), as well as subdural (15.7%) and extradural lesions (13.2%). Age, DM, and immunosuppression conditions are significantly associated with intracranial complications (p < 0.001, p = 0.018 and p = 0.022, respectively). Streptococcus infection is associated with intracranial complications (p = 0.001), although Staphylococcus and other microorganisms are not. Surgical intervention, mainly ESS, and broad-spectrum antibiotics remain the cornerstones of treatment.
Effects of Propofol on Cortical Electroencephalograms in the Operation of Glioma-Related Epilepsy
Authors: Li Xin , Wei Yu , Xie Yanfeng , Shi Quanhong , Zhan Yan , Dan Wei , Jiang Li
Published: March 2023

Background: A cortical electroencephalogram (ECoG) is often used for the intraoperative monitoring of epilepsy surgery, and propofol is an important intravenous anesthetic, but its effect on EEGs is unclear. Objectives: To further clarify the effect of propofol on cortical ECoGs during glioma-related epilepsy surgery and to clarify the possible clinical value. Methods: A total of 306 patients with glioma were included in the study. Two hundred thirty-nine with glioma-related epilepsy were included in the epilepsy group, and 67 without glioma-related epilepsy were included in the control group. All patients experienced continuous, real-time ECoG monitoring and long-term follow-up after surgery. Results: After injection of low-dose propofol, the rate of activated ECoGs in the epilepsy group (74%) was significantly higher than in the control group (9%). Furthermore, compared with patients in the untreated group, patients in the treated group had lower rates of early and long-term postoperative seizure frequencies and fewer interictal epileptiform discharges (IEDs). Conclusions: Low-dose infusion of propofol can specifically activate ECoGs in epilepsy patients. Therefore, activated ECoGs might provide an accurate and reliable method for identifying potential epileptic zones during glioma-related epilepsy surgery, resulting in better early and long-term prognoses after epilepsy surgery.
CircRNA RNA hsa_circ_0008234 Promotes Colon Cancer Progression by Regulating the miR-338-3p/ETS1 Axis and PI3K/AKT/mTOR Signaling
Authors: Wu Dejun , Li Yuqin , Xu Anjun , Tang Wenqing , Yu Bo
Published: March 2023

Circular RNAs (circRNAs) have been shown to play a crucial role in cancer occurrence and progression. This present work investigated the link between hsa_circ_0008234 and colon cancer. Data retrieved from GSE172229 was used to compare the circRNA profiles of colon cancer and surrounding non-tumorous tissues. The amount of RNA and protein in the molecules was determined using quantitative real-time PCR (qRT-PCR) and Western blot analysis, respectively. The cell proliferation ability was assessed using CCK8, EdU, colon formation, and nude mice tumorigenesis tests. Cell invasion and migration abilities were evaluated using transwell wound healing and mice lung metastasis model. Hsa_circ_0008234 piqued our interest because bioinformatics and qRT-PCR analyses revealed that it is upregulated in colon cancer tissue. Cell phenotypic studies suggest that hsa_circ_0008234 may significantly increase colon cancer cell aggressiveness. Mice experiments revealed that inhibiting hsa_circ_0008234 significantly reduced tumor growth and metastasis. Moreover, the fluorescence in situ hybridization experiment demonstrated that hsa_circ_0008234 is primarily found in the cytoplasm, implying that it potentially functions via a competitive endogenous RNA pathway. These findings indicated that hsa_circ_0008234 may act as a “molecular sponge” for miR-338-3p, increasing the expression of miR-338-target 3p’s ETS1. In addition, the traditional oncogenic pathway PI3K/AKT/mTOR signaling was found to be the potential downstream pathway of the hsa_circ_0008234/miR-338-3p/ETS1 axis. In conclusion, hsa_circ_0008234 increases colon cancer proliferation, infiltration, and migration via the miR-338-3p/ETS1/PI3K/AKT axis; therefore, it could serve as a target and a focus for colon cancer therapy.
KRAS Mutations Are Associated with Shortened Survival in Patients with Epithelioid Malignant Pleural Mesothelioma
Authors: Vannucchi Margherita , Pennati Veronica , Mencaroni Clelia , Defraia Chiara , Bardhi Ledi , Castiglione Francesca , Bellan Cristiana , Comin Camilla Eva
Published: March 2023

Malignant pleural mesothelioma (MPM) is an aggressive malignancy of the pleural surface that includes three major histologic subtypes, epitheliod, sarcomatoid and biphasic. Epithelioid mesothelioma is usually associated with better prognosis. The genetic mechanisms driving MPM, the possible target mutations and the correlation with overall survival remain largely unsettled. We performed target exome sequencing in 29 cases of MPM aimed at identifying somatic mutations and, eventually, their correlation with phenotypic traits and prognostic significance. We found that KRAS mutations, occurring in 13.7% of cases, were associated with shortened median survival (7.6 versus 32.6 months in KRAS wild-type; p = 0.005), as it was the occurrence of any ≥3 mutations (7.6 versus 37.6 months; p = 0.049). Conversely, the presence of KDR single nucleotide polymorphism p.V297I (rs2305948) resulted in a favorable variable for survival (NR versus 23.4 months; p = 0.026). With the intrinsic limitations of a small number of cases and patient heterogeneity, results of this study contribute to the characterization of the mutation profile of MPM and the impact of selected somatic mutations, and possibly KDR polymorphism, on prognosis.
Lung Cancer Screening Eligibility and Referral Practices in Texas Organizations Serving People with Substance Use Disorders
Authors: Britton Maggie , Chen Tzuan A. , Martinez Leal Isabel , Rogova Anastasia , Kyburz Bryce , Williams Teresa , Patel Mayuri , El-Zein Randa , Bernicker Eric H. , Lowenstein Lisa M. , Reitzel Lorraine R.
Published: March 2023

For people at elevated risk for lung cancer, lung cancer screening (LCS) reduces lung cancer mortality. People with non-nicotine substance use disorders (SUDs) have elevated rates of smoking compared with the general population, highlighting them as a priority population for LCS consideration. Although research has shown LCS is underutilized, there is little literature to inform whether organizations that serve individuals with SUDs have existing clinical protocols surrounding LCS. In the current study, we examine the LCS eligibility and referral practices among these organizations. We conducted a statewide needs assessment survey in 2021 to discern how tobacco use was being addressed at Texas organizations that provide treatment or services to individuals with SUDs. Respondents were asked to report on their center’s LCS eligibility and referral practices. The analytic sample consists of 125 respondents who represented 23 federally qualified health centers, 29 global local mental health authorities (LMHAs), 12 substance use treatment programs in LMHAs, and 61 standalone substance use treatment centers. Very few respondents indicated that healthcare providers at their center made referrals to LCS for patients (8.8%); a few respondents indicated that their healthcare providers assessed patients’ eligibility for LCS but did not make referrals (3.2%). Intervention and implementation efforts are needed in these and other SUD healthcare settings to bolster organizational capacity and ensure that patients are being navigated to lung cancer screening at multiple touch points across the care continuum.
Survival Differences by Comorbidity Burden among Patients with Stage I/II Non-Small-Cell Lung Cancer after Thoracoscopic Resection
Authors: Wheeler Meghann , Karanth Shama D. , Mehta Hiren J. , Yang Danting , Aduse-Poku Livingstone , Washington Caretia , Hong Young-Rock , Zhang Dongyu , Gould Michael K. , Braithwaite Dejana
Published: March 2023

We sought to compare overall survival (OS) by comorbidity burden among patients with stage I/II non-small cell lung cancer (NSCLC) who received thoracoscopic resection. Utilizing data from the National Cancer Database, we conducted a survival analysis among patients aged 50+ with stage I/II NSCLC who received thoracoscopic resection between 2010 and 2017. The comorbidity burden was measured by the Charlson comorbidity index (CCI, 0, 1, 2+). Multivariable Cox proportional hazard models were used to compare overall survival relative to the CCI (CCI of 0 as the referent). Subgroup analyses were conducted considering sex, age groups, days from diagnosis to surgery, facility type, laterality, and type of surgery. For this study, 61,760 patients were included, with a mean age of 69.1 years (SD: 8.5). Notably, 51.2% had a CCI of 0, 31.8% had a CCI of 1, and 17.0% had a CCI of 2+. Most participants were non-Hispanic White (87.5%), and 56.9% were female. We found that an increase in the CCI was associated with a higher risk of all-cause mortality (CCI 1 vs. 0 aHR: 1.24, 95% CI: 1.20–1.28; CCI 2+ vs. 0 aHR: 1.51, 95% CI: 1.45–1.57; p-trend < 0.01). Our subgroup analysis according to sex suggested that the association between CCI and risk of death was stronger in women.
Lymph Node Log-Odds Ratio Accurately Defines Prognosis in Resectable Non-Small Cell Lung Cancer
Authors: Benej Michal , Klikovits Thomas , Krajc Tibor , Bohanes Tomas , Schulte Lisa , Hochmair Maximilian Johannes , Watzka Stefan , Mosleh Berta , Hoetzenecker Konrad , Aigner Clemens , Hoda Mir Alireza , Mueller Michael Rolf
Published: March 2023

Objectives: The ratio of positive and resected lymph nodes (LN ratio) has been shown to be prognostic in non-small cell lung cancer (NSCLC). Contrary to the LN ratio, calculating the LN log-odds ratio (LN-LOR) additionally considers the total number of resected lymph nodes. We aim to evaluate LN-LOR between positive and resected lymph nodes as a prognostic factor in operable NSCLC. Methods: Patients with NSCLC who underwent curative intent lobectomy treated at two high-volume centers were retrospectively studied. LN-LOR was dichotomized according to impact on OS and further combined with N descriptors and correlated with clinical variables and survival. Results: 944 patients were included. Cut-off analysis revealed that an LN-LOR of −0.34 significantly discriminated patients according to OS (p < 0.001, chi-squared test 41.26). When combined with N1 and N2 descriptors, LN-LOR low risk (median OS not reached and 83 months) and LN-LOR high-risk patients (median OS 50 and 59 months) had similar survival irrespective of the anatomical location of the positive lymph nodes. Multivariable Cox regression analysis revealed that age (HR 1.02, 95% CI 1.001–1.032), sex (male, HR 1.65, 95% CI 1.25–2.19), histological subtype (HR 2.11, 95% CI 1.35–3.29), pathological stage (HR 1.23, 95% CI 1.01–1.45) and LN-LOR risk groups (low risk, HR 0.48, 95% CI 0.32–0.72) were independent prognostic factors for OS. Conclusions: This retrospective two-center analysis shows that LN-LOR is significantly associated with OS in resectable NSCLC and might better reflect the biological behavior of the disease, regardless of anatomical lymph node locations. This finding may additionally support the value of extensive LN dissection.
Iron and Cancer: A Special Issue
Authors: Okazaki Yasumasa , Hino Keisuke
Published: March 2023

Iron is an essential element for all organisms, and iron-containing proteins play critical roles in cellular functions [...]
The Role of the Gut Microbiome in Cancer Immunotherapy: Current Knowledge and Future Directions
Authors: Kiousi Despoina E. , Kouroutzidou Antonia Z. , Neanidis Konstantinos , Karavanis Emmanuel , Matthaios Dimitrios , Pappa Aglaia , Galanis Alex
Published: March 2023

Cancer immunotherapy is a treatment modality that aims to stimulate the anti-tumor immunity of the host to elicit favorable clinical outcomes. Immune checkpoint inhibitors (ICIs) gained traction due to the lasting effects and better tolerance in patients carrying solid tumors in comparison to conventional treatment. However, a significant portion of patients may present primary or acquired resistance (non-responders), and thus, they may have limited therapeutic outcomes. Resistance to ICIs can be derived from host-related, tumor-intrinsic, or environmental factors. Recent studies suggest a correlation of gut microbiota with resistance and response to immunotherapy as well as with the incidence of adverse events. Currently, preclinical and clinical studies aim to elucidate the unique microbial signatures related to ICI response and anti-tumor immunity, employing metagenomics and/or multi-omics. Decoding this complex relationship can provide the basis for manipulating the malleable structure of the gut microbiota to enhance therapeutic success. Here, we delve into the factors affecting resistance to ICIs, focusing on the intricate gut microbiome–immunity interplay. Additionally, we review clinical studies and discuss future trends and directions in this promising field.
p53 and p63 Proteoforms Derived from Alternative Splicing Possess Differential Seroreactivity in Colorectal Cancer with Distinct Diagnostic Ability from the Canonical Proteins
Authors: Montero-Calle Ana , Garranzo-Asensio María , Torrente-Rodríguez Rebeca M. , Ruiz-Valdepeñas Montiel Víctor , Poves Carmen , Dziaková Jana , Sanz Rodrigo , Díaz del Arco Cristina , Pingarrón José Manuel , Fernández-Aceñero María Jesús , Campuzano Susana , Barderas Rodrigo
Published: March 2023

Colorectal cancer (CRC) is the third most common cancer and the second most frequent cause of cancer-related death worldwide. The detection in plasma samples of autoantibodies against specific tumor-associated antigens has been demonstrated to be useful for the early diagnosis of CRC by liquid biopsy. However, new studies related to the humoral immune response in cancer are needed to enable blood-based diagnosis of the disease. Here, our aim was to characterize the humoral immune response associated with the different p53 and p63 proteoforms derived from alternative splicing and previously described as aberrantly expressed in CRC. Thus, here we investigated the diagnostic ability of the twelve p53 proteoforms and the eight p63 proteoforms described to date, and their specific N-terminal and C-terminal end peptides, by means of luminescence HaloTag beads immunoassays. Full-length proteoforms or specific peptides were cloned as HaloTag fusion proteins and their seroreactivity analyzed using plasma from CRC patients at stages I-IV (n = 31), individuals with premalignant lesions (n = 31), and healthy individuals (n = 48). p53γ, Δ40p53β, Δ40p53γ, Δ133p53γ, Δ160p53γ, TAp63α, TAp63δ, ΔNp63α, and ΔNp63δ, together with the specific C-terminal end α and δ p63 peptides, were found to be more seroreactive against plasma from CRC patients and/or individuals with premalignant lesions than from healthy individuals. In addition, ROC (receiver operating characteristic) curves revealed a high diagnostic ability of those p53 and p63 proteoforms to detect CRC and premalignant individuals (AUC higher than 85%). Finally, electrochemical biosensing platforms were employed in POC-like devices to investigate their usefulness for CRC detection using selected p53 and p63 proteoforms. Our results demonstrate not only the potential of these biosensors for the simultaneous analysis of proteoforms’ seroreactivity, but also their convenience and versatility for the clinical detection of CRC by liquid biopsy. In conclusion, we here show that p53 and p63 proteoforms possess differential seroreactivity in CRC patients in comparison to controls, distinctive from canonical proteins, which should improve the diagnostic panels for obtaining a blood-based biomarker signature for CRC detection.
Wellbeing and Complementary Therapies in Breast Cancer Peripheral Neuropathy Care: A Scoping Review Focused on Foot Health
Authors: Veiga-Seijo Raquel , Perez-Lopez Maria Eva , Fernandez-Lopez Uxia , Mosquera-Fernandez Abian , Seijo-Bestilleiro Rocio , Gonzalez-Martin Cristina
Published: March 2023

Background: Chemotherapy-induced peripheral neuropathy is a multidimensional health problem. Up to now, little evidence has been found concerning its impact on quality of life and foot health. Evaluation tools and prevention and treatment strategies must be reported. This study aimed to map the literature on the impact of this side effect on the wellbeing and foot health of people with breast cancer and to describe their main assessment strategies and complementary therapies. Methods: A scoping review was carried out while following the PRISMA-ScR and Arksey and O’Malley guidelines. Different databases (Cochrane Plus, Scopus, Web of Science, and Pubmed) were used. A total of 221 results were identified. Sixteen articles were included. Results: The thematic analysis obtained the following categories: the impact of peripheral neuropathy on quality of life and foot health, complementary therapies as a path for new strategies, and the need for clinicians and researchers to get involved in researching this side effect. Conclusions: Peripheral neuropathy has a negative impact on people’s quality of life. Implications for foot health and maintaining an active and healthy lifestyle have not been previously reported. Complementary therapies are recommended by scientific evidence, highlighting exercise. However, there is a need to develop more research that will help to incorporate them into evidence-based practice.
Spontaneous Sepsis in Adult Horses: From Veterinary to Human Medicine Perspectives
Authors: Blangy-Letheule Angélique , Vergnaud Amandine , Dupas Thomas , Rozec Bertrand , Lauzier Benjamin , Leroux Aurélia A.
Published: March 2023

Sepsis is a life-threatening disease defined as an organ dysfunction caused by a dysregulated host response to an infection. Early diagnosis and prognosis of sepsis are necessary for specific and timely treatment. However, no predictive biomarkers or therapeutic targets are available yet, mainly due to the lack of a pertinent model. A better understanding of the pathophysiological mechanisms associated with sepsis will allow for earlier and more appropriate management. For this purpose, experimental models of sepsis have been set up to decipher the progression and pathophysiology of human sepsis but also to identify new biomarkers or therapeutic targets. These experimental models, although imperfect, have mostly been performed on a murine model. However, due to the different pathophysiology of the species, the results obtained in these studies are difficult to transpose to humans. This underlines the importance of identifying pertinent situations to improve patient care. As humans, horses have the predisposition to develop sepsis spontaneously and may be a promising model for spontaneous sepsis. This review proposes to give first an overview of the different animal species used to model human sepsis, and, secondly, to focus on adult equine sepsis as a spontaneous model of sepsis and its potential implications for human and veterinary medicine.
ABCB1 and ABCG2 Overexpression Mediates Resistance to the Phosphatidylinositol 3-Kinase Inhibitor HS-173 in Cancer Cell Lines
Authors: Wu Chung-Pu , Hung Cheng-Yu , Hsieh Ya-Ju , Murakami Megumi , Huang Yang-Hui , Su Tsung-Yao , Hung Tai-Ho , Yu Jau-Song , Wu Yu-Shan , Ambudkar Suresh V.
Published: March 2023

Constitutive activation of the phosphoinositide-3-kinase (PI3K)/Akt signaling pathway is crucial for tumor growth and progression. As such, this pathway has been an enticing target for drug discovery. Although HS-173 is a potent PI3K inhibitor that halts cancer cell proliferation via G2/M cell cycle arrest, the resistance mechanisms to HS-173 have not been investigated. In this study, we investigated the susceptibility of HS-173 to efflux mediated by the multidrug efflux transporters ABCB1 and ABCG2, which are two of the most well-known ATP-binding cassette (ABC) transporters associated with the development of cancer multidrug resistance (MDR). We found that the overexpression of ABCB1 or ABCG2 significantly reduced the efficacy of HS-173 in human cancer cells. Our data show that the intracellular accumulation of HS-173 was substantially reduced by ABCB1 and ABCG2, affecting G2/M arrest and apoptosis induced by HS-173. More importantly, the efficacy of HS-173 in multidrug-resistant cancer cells could be recovered by inhibiting the drug-efflux function of ABCB1 and ABCG2. Taken together, our study has demonstrated that HS-173 is a substrate for both ABCB1 and ABCG2, resulting in decreased intracellular concentration of this drug, which may have implications for its clinical use.
Cracking the Code of Neuronal Cell Fate
Authors: Morello Giovanna , La Cognata Valentina , Guarnaccia Maria , D'Agata Velia , Cavallaro Sebastiano
Published: March 2023

Transcriptional regulation is fundamental to most biological processes and reverse-engineering programs can be used to decipher the underlying programs. In this review, we describe how genomics is offering a systems biology-based perspective of the intricate and temporally coordinated transcriptional programs that control neuronal apoptosis and survival. In addition to providing a new standpoint in human pathology focused on the regulatory program, cracking the code of neuronal cell fate may offer innovative therapeutic approaches focused on downstream targets and regulatory networks. Similar to computers, where faults often arise from a software bug, neuronal fate may critically depend on its transcription program. Thus, cracking the code of neuronal life or death may help finding a patch for neurodegeneration and cancer.
Approximate Content-Addressable Memories: A Review
Authors: Garzón Esteban , Yavits Leonid , Teman Adam , Lanuzza Marco
Published: March 2023

Content-addressable memory (CAM) has been part of the memory market for more than five decades. CAM can carry out a single clock cycle lookup based on the content rather than an address. Thanks to this attractive feature, CAM is utilized in memory systems where a high-speed content lookup technique is required. However, typical CAM applications only support exact matching, as opposed to approximate matching, where a certain Hamming distance (several mismatching characters between a query pattern and the dataset stored in CAM) needs to be tolerated. Recent interest in approximate search has led to the development of new CAM-based alternatives, accelerating the processing of large data workloads in the realm of big data, genomics, and other data-intensive applications. In this review, we provide an overview of approximate CAM and describe its current and potential applications that would benefit from approximate search computing.