bluuebunny/crossref_metadata_2025 huggingface dataset:
Screening NOx Storage Performance—Demonstrating a High Throughput Approach for Evaluating Emission Control Catalysts under Transient Conditions
Authors: Sundermann Andreas , Kögel Markus , Gerlach Olga
Published: September 2019

At hte the high throughput (HT) approach is applied in the field of environmental catalysis on a routine basis. Research programs for automotive applications require validated screening protocols for conditions relevant to engine exhaust as well as experimental measures to ensure quality control using statistical design of experiment. To illustrate the HT approach for a test protocol with dynamic feed switches in a 48-fold reactor, 15 model catalysts for lean NOx traps (LNT) were prepared and screened fresh and after 800 °C hydrothermal aging. In the fresh state, highest NOx efficiency was found at 350–450 °C. A ranking of BaO > SrO > CaO was found as the most active NOx storage components when used as dopants on alumina. 800 °C aging results in a severe performance loss. Using XRD and BET analysis, Pt sintering is identified as most likely cause. These findings agree well with results from conventional tests reported in the literature.
Therapeutic Approaches Targeting Nucleolus in Cancer
Authors: Carotenuto Pietro , Pecoraro Annalisa , Palma Gaetano , Russo Giulia , Russo Annapina
Published: September 2019

The nucleolus is a distinct sub-cellular compartment structure in the nucleus. First observed more than 200 years ago, the nucleolus is detectable by microscopy in eukaryotic cells and visible during the interphase as a sub-nuclear structure immersed in the nucleoplasm, from which it is not separated from any membrane. A huge number of studies, spanning over a century, have identified ribosome biogenesis as the main function of the nucleolus. Recently, novel functions, independent from ribosome biogenesis, have been proposed by several proteomic, genomic, and functional studies. Several works have confirmed the non-canonical role for nucleoli in regulating important cellular processes including genome stability, cell-cycle control, the cellular senescence, stress responses, and biogenesis of ribonucleoprotein particles (RNPs). Many authors have shown that both canonical and non-canonical functions of the nucleolus are associated with several cancer-related processes. The association between the nucleolus and cancer, first proposed by cytological and histopathological studies showing that the number and shape of nucleoli are commonly altered in almost any type of cancer, has been confirmed at the molecular level by several authors who demonstrated that numerous mechanisms occurring in the nucleolus are altered in tumors. Recently, therapeutic approaches targeting the nucleolus in cancer have started to be considered as an emerging “hallmark” of cancer and several therapeutic interventions have been developed. This review proposes an up-to-date overview of available strategies targeting the nucleolus, focusing on novel targeted therapeutic approaches. Finally, a target-based classification of currently available treatment will be proposed.
Single Cell Mass Cytometry of Non-Small Cell Lung Cancer Cells Reveals Complexity of In Vivo and Three-Dimensional Models over the Petri-Dish
Authors: Alföldi Róbert , Balog József Á. , Faragó Nóra , Halmai Miklós , Kotogány Edit , Neuperger Patrícia , Nagy Lajos I. , Fehér Liliána Z. , Szebeni Gábor J. , Puskás László G.
Published: September 2019

Single cell genomics and proteomics with the combination of innovative three-dimensional (3D) cell culture techniques can open new avenues toward the understanding of intra-tumor heterogeneity. Here, we characterize lung cancer markers using single cell mass cytometry to compare different in vitro cell culturing methods: two-dimensional (2D), carrier-free, or bead-based 3D culturing with in vivo xenografts. Proliferation, viability, and cell cycle phase distribution has been investigated. Gene expression analysis enabled the selection of markers that were overexpressed: TMEM45A, SLC16A3, CD66, SLC2A1, CA9, CD24, or repressed: EGFR either in vivo or in long-term 3D cultures. Additionally, TRA-1-60, pan-keratins, CD326, Galectin-3, and CD274, markers with known clinical significance have been investigated at single cell resolution. The described twelve markers convincingly highlighted a unique pattern reflecting intra-tumor heterogeneity of 3D samples and in vivo A549 lung cancer cells. In 3D systems CA9, CD24, and EGFR showed higher expression than in vivo. Multidimensional single cell proteome profiling revealed that 3D cultures represent a transition from 2D to in vivo conditions by intermediate marker expression of TRA-1-60, TMEM45A, pan-keratin, CD326, MCT4, Gal-3, CD66, GLUT1, and CD274. Therefore, 3D cultures of NSCLC cells bearing more putative cancer targets should be used in drug screening as the preferred technique rather than the Petri-dish.
Investigation of the Oxidation Mechanism of Dopamine Functionalization in an AZ31 Magnesium Alloy for Biomedical Applications
Authors: Ghanbari Arezoo , Warchomicka Fernando , Sommitsch Christof , Zamanian Ali
Published: September 2019

Implant design and functionalization are under significant investigation for their ability to enhance bone-implant grafting and, thus, to provide mechanical stability for the device during the healing process. In this area, biomimetic functionalizing polymers like dopamine have been proven to be able to improve the biocompatibility of the material. In this work, the dip coating of dopamine on the surface of the magnesium alloy AZ31 is investigated to determine the effects of oxygen on the functionalization of the material. Two different conditions are applied during the dip coating process: (1) The absence of oxygen in the solution and (2) continuous oxygenation of the solution. Energy dispersive spectroscopy (EDS) and Fourier transform infrared spectroscopy (FTIR) are used to analyze the composition of the formed layers, and the deposition rate on the substrate is determined by molecular dynamic simulation. Electrochemical analysis and cell cultivation are performed to determine the corrosion resistance and cell’s behavior, respectively. The high oxygen concentration in the dopamine solution promotes a homogeneous and smooth coating with a drastic increase of the deposition rate. Also, the addition of oxygen into the dip coating process increases the corrosion resistance of the material.
A Mixed Methods Approach to Culture-Sensitive Academic Self-Concept Research
Authors: Rüschenpöhler Lilith , Markic Silvija
Published: September 2019

(1) Background: In self-concept research, Likert scales are still relied upon despite the fact that they pose methodological difficulties for research in culturally diverse societies. This calls the validity of the data into question. In the present study, we develop a mixed methods design for culture-sensitive academic self-concept research. We test it in a study about chemistry self-concept with secondary school students; (2) Methods: Interview (N = 43) and questionnaire (N = 116) data were collected; (3) Results: The mixed methods approach allowed connecting self-concept with culturally shaped narratives: in the quantitative data, we found the well-documented gender gap in favor of boys. However, among the students with a Turkish migration background, the girls showed stronger chemistry self-concepts. The interviews suggested that girls with Turkish migration background find it easier to connect their chemistry learning to their personal life than the boys with Turkish migration background; (4) Conclusion: Based on further literature, we hypothesize that these differences might be due to a less masculine conception of science in the Turkish society. The mixed methods approach allows detecting measurement bias, which increases the validity of science self-concept data in culturally diverse contexts.
Kick Risk Forecasting and Evaluating During Drilling Based on Autoregressive Integrated Moving Average Model
Authors: Yin Hu , Si Menghan , Li Qian , Zhang Jinke , Dai Liming
Published: September 2019

Timely forecasting of the kick risk after a well kick can reduce the waiting time after well shut-in and provide more time for well killing operations. At present, the multiphase flow model is used to simulate and forecast the pit gain and casing pressure. Due to the complexity of downhole conditions, calculation of the multiphase flow model is difficult. In this paper, the time series analysis method is used to excavate the information contained in the time-varying data of pit gain and casing pressure. A forecasting model based on a time series analysis method of pit gain and casing pressure is established to forecast the pit gain and casing pressure after a kick. To divide the kick risk level and achieve the forecasting of the kick risk before and after well shut-in, kick risk analysis plates based on pit gain and casing pressure are established. Three pit gain cases and one casing pressure case are studied, and a comparison between measured data and predicted data shows that the proposed method has high prediction accuracy and repeatability.
Influence of Measurement Aggregation Algorithms on Power Quality Assessment and Correlation Analysis in Electrical Power Network with PV Power Plant
Authors: Jasiński Michał , Sikorski Tomasz , Kostyła Paweł , Kaczorowska Dominika , Leonowicz Zbigniew , Rezmer Jacek , Szymańda Jarosław , Janik Przemysław , Bejmert Daniel , Rybiański Marek , Jasińska Elżbieta
Published: September 2019

Recently a number of changes were introduced in amendment to standard EN 50160 related to power quality (PQ) including 1 min aggregation intervals and the obligation to consider 100% of measured data taken for the assessment of voltage variation in a low voltage (LV) supply terminal. Classical power quality assessment can be extended using a correlation analysis so that relations between power quality parameters and external indices such as weather conditions or power demand can be revealed. This paper presents the results of a comparative investigation of the application of 1 and 10 min aggregation times in power quality assessment as well as in the correlation analysis of power quality parameters and weather conditions and the energy production of a 100 kW photovoltaic (PV) power plant connected to a LV network. The influence of the 1 min aggregation time on the result of the PQ assessment as well as the correlation matrix in comparison with the 10 min aggregation algorithm is presented and discussed.
The Effect of Calcium, Citrate, and Urea on the Stability of Ultra-High Temperature Treated Milk: A Full Factorial Designed Study
Authors: Karlsson Maria A. , Lundh Åse , Innings Fredrik , Höjer Annika , Wikström Malin , Langton Maud
Published: September 2019

The composition of raw milk is important for the stability of dairy products with a long shelf-life. Based on known historical changes in raw milk composition, the aim of this study was to get a better understanding of how possible future variations in milk composition may affect the stability of dairy products. The effects of elevated calcium, citrate, and urea levels on the stability of ultra-high temperature (UHT) treated milk stored for 52 weeks at 4, 20, 30, and 37 °C were investigated by a two-level full factorial designed study with fat separation, fat adhesion, sedimentation, color, pH, ethanol stability, and heat coagulation time as response variables. The results showed that elevated level of calcium lowered the pH, resulting in sedimentation and significantly decreased stability. Elevated level of citrate was associated with color, but the stability was not improved compared to the reference UHT milk. Elevated levels of urea or interaction terms had little effect on the stability of UHT milk. Storage conditions significantly affected the stability. In conclusion, to continue produce dairy products with high stability, the dairy industry should make sure the calcium content of raw milk is not too high and that storage of the final product is appropriate.
Communicating Arsenic’s Risks
Authors: Rogers Shannon H. , Rardin Laurie R. , Lawlor Kathrin , Chen Celia Y. , Borsuk Mark. E.
Published: September 2019

Arsenic is a naturally occurring toxic metalloid that has many human health implications. Its strong prevalence in the bedrock and thus much of the well water in New England puts many private well owners at risk. It is also found in food products, particularly those that contain rice. Despite the documented health risks, arsenic is not high on the list of concerns for residents of the region. This study will describe two types of environmental communication efforts that have been undertaken by the Dartmouth Toxic Metals Superfund Research Program (DTMSRP)—the development and evaluation of a comprehensive website, Arsenic and You, and a mental models research approach to better understand the disconnect between expert and community perceptions of arsenic risk. We find that there are knowledge gaps between the two, particularly regarding the origin of arsenic in drinking water and food, the necessity of testing well water, and the process for treating water that is above recommended limits. Moreover, the mental models approach provides a structured framework for better understanding these gaps. A website can address some of these disconnects, and it is important to have a “one-stop shop” for vetted information on the risks and steps to reduce exposure.
Dementia Patient Segmentation Using EMR Data Visualization: A Design Study
Authors: Ha Hyoji , Lee Jihye , Han Hyunwoo , Bae Sungyun , Son Sangjoon , Hong Changhyung , Shin Hyunjung , Lee Kyungwon
Published: September 2019

(1) Background: The Electronic Medical Record system, which is a digital medical record management architecture, is critical for reliable medical research. It facilitates the investigation of disease patterns and efficient treatment via collaboration with data scientists. (2) Methods: In this study, we present multidimensional visual tools for the analysis of multidimensional datasets via a combination of 3-dimensional radial coordinate visualization (3D RadVis) and many-objective optimization (e.g., Parallel Coordinates). Also, we propose a user-driven research design to facilitate visualization. We followed a design process to (1) understand the demands of domain experts, (2) define the problems based on relevant works, (3) design visualization, (4) implement visualization, and (5) enable qualitative evaluation by domain experts. (3) Results: This study provides clinical insight into dementia based on EMR data via visual analysis. Results of a case study based on questionnaires surveying daily living activities indicated that daily behaviors influenced the progression of dementia. (4) Conclusions: This study provides a visual analytical tool to support cluster segmentation. Using this tool, we segmented dementia patients into clusters and interpreted the behavioral patterns of each group. This study contributes to biomedical data interpretation based on a visual approach.
Evaluation of Power Production Asymmetry during Cycling in Persons with Multiple Sclerosis
Authors: Farrell John W. , Bemben Debra A. , Black Christopher D. , Larson Daniel J. , Pardo Gabriel , Fjeldstad-Pardo Cecilie , Larson Rebecca D.
Published: September 2019

Lower limb asymmetries have been observed in persons with multiple sclerosis (PwMS), and have been associated with mobility impairment. An incremental cycling test was performed on a cycle ergometer to determine peak power output (PPO) and peak oxygen consumption (VO2peak). Then, participants cycled at 50%, 60%, and 70% of their PPO to assess the contribution of each lower limb to power production. Two-way repeated measures ANOVA was used to detect group × intensity differences in power production asymmetry. Eight PwMS and six healthy individuals (Non-MS) completed the study. No statistically significant (p > 0.05) group × intensity interactions or main effects were present when examining between-limb differences in power production. The current data do not indicate a statistically significant difference in power production asymmetry between groups and exercise intensities. Previous research has established a 10% difference between contralateral limbs as a threshold for asymmetry. The average asymmetry in power production in PwMS exceeded the 10% threshold at all measured outputs, suggesting the presence of asymmetry in power production.
Identifying the Factors Related to Depressive Symptoms Amongst Community-Dwelling Older Adults with Mild Cognitive Impairment
Authors: Song Dan , Yu Doris S.F. , Li Polly W.C. , Sun Qiuhua
Published: September 2019

High-level depressive symptoms have been reported in individuals with mild cognitive impairment (MCI), resulting in increased risk of progression to dementia. However, studies investigating the correlates of depressive symptoms among this population are scarce. This study aimed to investigate the significant socio-demographic, lifestyle-related and disease-related correlates of depressive symptoms among this cohort. Cross-sectional data were obtained from a sample of 154 Chinese community-dwelling older adults with MCI. MCI subjects were screened by the Montreal Cognitive Assessment. Depressive symptoms were measured by the Geriatric Depression Scale. Possible correlates of depressive symptoms in individuals with MCI were explored by multiple linear regressions. The prevalence of depressive symptoms among Chinese older adults with MCI was 31.8%. In multiple regression analysis, poor perceived positive social interaction, small social network, low level of physical activity, poor functional status, subjective memory complaint, and poor health perception were correlated with depressive symptoms. The findings highlight that depressive symptoms are sufficient to warrant evaluation and management in older adults with MCI. Addressing social isolation, assisting this vulnerable group in functional and physical activities, and cultivating a positive perception towards cognitive and physical health are highly prioritized treatment targets among individuals with MCI.
New Eye Lens Dose Limit: Status of Knowledge in Campania Hospitals
Authors: D’Avino Vittoria , Angrisani Leopoldo , La Verde Giuseppe , Pugliese Mariagabriella , Raulo Adelaide , Sabatino Giuseppe , Coppola Fulvio
Published: September 2019

The International Commission on Radiation Protection (ICRP) in 2011 recommended the lowering of the annual eye lens dose limit from 150 mSv/year to 20 mSv/year in order to reduce the risk of X-ray-induced lens opacity in medical staff. The purpose of this study was to assess the status of knowledge of the new eye lens dose limit and of the radioprotection culture among operators. To this end, a questionnaire was administered to physicians, X-ray technicians, and nurses working in five hospitals of the Campania region, Italy. A total of 64 questionnaires were collected in the hospital departments in which procedures involving ionizing radiation were routinely performed. The data analyzed yielded the following results: 12 operators affirmed to know the new eye lens dose limit, 53 operators routinely wore lead aprons, and 23 operators used lead glasses. Four workers performed eye lens dosimetry through specific dosimeters. A significant lack of knowledge of the reduced eye lens dose limit suggests the need to implement radioprotection-training programs aimed at raising awareness about the importance of health care in the workplace and at reducing the risk of radio-induced effects to the eye lens.
Move-It: A Cluster-Randomised Digital Worksite Exercise Intervention in China: Outcome and Process Evaluation
Authors: Blake Holly , Lai Betsy , Coman Emil , Houdmont Jonathan , Griffiths Amanda
Published: September 2019

We evaluate the outcomes and processes of a video and web-based worksite exercise intervention for sedentary office workers in China, in a 2-arm cluster-randomised wait-list control trial (n = 282: intervention (INT) n = 196 and wait-list control (WLC) n = 86). Eligible clusters were two sites of a major organisation in China randomly allocated to each group (INT: Guangzhou; WLC: Beijing); eligible participants were site employees (n = 690). A theoretically informed digital workplace intervention (Move-It) involving a 10 min Qigong exercise session (video demonstration via website) was delivered twice a day at set break times during the working day for 12 consecutive weeks. Individual-level outcomes were assessed. Participants’ physical activity increased significantly from baseline to post-intervention similarly in both the intervention and the control group. There was a significantly smaller increase in weekday sitting hours in intervention than controls (by 4.66 h/week), and work performance increased only in the control group. Process evaluation (including six focus groups) was conducted using the RE-AIM (reach, effectiveness, adoption, implementation and maintenance) framework. The intervention had wide reach and was successfully marketed to all employees with good uptake. The participatory approach increased perceived organisational support and enhanced adoption. The intervention was implemented broadly as planned. Qigong worksite exercise intervention can be successfully delivered to sedentary office workers in China using video and web-based platforms. It may increase physical activity and does not adversely affect perceived work performance. The study highlights the complexity of conducting health promotion research in real-world organisational settings.
Dynamic Changes of NDVI in the Growing Season of the Tibetan Plateau During the Past 17 Years and Its Response to Climate Change
Authors: Huang Xianglin , Zhang Tingbin , Yi Guihua , He Dong , Zhou Xiaobing , Li Jingji , Bie Xiaojuan , Miao Jiaqing
Published: September 2019

The fragile alpine vegetation in the Tibetan Plateau (TP) is very sensitive to environmental changes, making TP one of the hotspots for studying the response of vegetation to climate change. Existing studies lack detailed description of the response of vegetation to different climatic factors using the method of multiple nested time series analysis and the method of grey correlation analysis. In this paper, based on the Normalized Difference Vegetation Index (NDVI) of TP in the growing season calculated from the MOD09A1 data product of Moderate-resolution Imaging Spectroradiometer (MODIS), the method of multiple nested time series analysis is adopted to study the variation trends of NDVI in recent 17 years, and the lag time of NDVI to climate change is analyzed using the method of Grey Relational Analysis (GRA). Finally, the characteristics of temporal and spatial differences of NDVI to different climate factors are summarized. The results indicate that: (1) the spatial distribution of NDVI values in the growing season shows a trend of decreasing from east to west, and from north to south, with a change rate of −0.13/10° E and −0.30/10° N, respectively. (2) From 2001 to 2017, the NDVI in the TP shows a slight trend of increase, with a growth rate of 0.01/10a. (3) The lag time of NDVI to air temperature is not obvious, while the NDVI response lags behind cumulative precipitation by zero to one month, relative humidity by two months, and sunshine duration by three months. (4) The effects of different climatic factors on NDVI are significantly different with the increase of the study period.
Analysis of Epidemiological Characteristics of Scarlet Fever in Zhejiang Province, China, 2004–2018
Authors: Lu Qinbao , Wu Haocheng , Ding Zheyuan , Wu Chen , Lin Junfen
Published: September 2019

Objective: The aim of this study was to analyze the trends and epidemiological characteristics of scarlet fever in Zhejiang Province in 2004–2018, intending to provide a basis for targeted prevention and control of this disease. Method: We collated the epidemiological data for cases of scarlet fever from the China Information System for Disease Control and Prevention (CISDCP) in Zhejiang province between 1 January 2004 and 31 December 2018. Descriptive statistical analysis was used to analyze epidemiological characteristics of scarlet fever, whereas the Getis-Ord Gi* statistic was used to determine the hotspot incidence of scarlet fever. Results: In 2004–2018, a total of 22,194 cases of scarlet fever were reported in Zhejiang Province, with no death reports. The annual average of scarlet fever incidence was 2.82/100,000 (range,1.12 to 6.34/100,000). The male incidence was higher than that among female (χ2 = 999.834, p < 0.05), and a majority of the cases (86.42%) occurred in children aged 3–9 years. Each year, the incidence of scarlet fever in Zhejiang Province appeared two seasonal peaks: the first peak occurred from March to June (the constituent ratio was 49.06%), the second peak was lower than the first one during November and the following January (the constituent ratio was 28.67%). The two peaks were almost in accordance with the school spring semester and autumn–winter semester, respectively. The incidence in the northern regions of the province was generally higher than that in the southern regions. High-value clusters were detected in the central and northern regions, while low-value clusters occurred in the southern regions via the Getis-Ord Gi* statistical analysis. Conclusions: The prevalence of scarlet fever in Zhejiang Province showed a marked seasonality variation and mainly clustered in the central and northern regions in 2004–2018. Children under 15 years of age were most susceptible to scarlet fever. Kindergartens and primary schools should be the focus of prevention and control, and targeted strategies and measures should be taken to reduce the incidence.
Effect of Same-Sex Marriage Referendums on the Suicidal Ideation Rate among Nonheterosexual People in Taiwan
Authors: Lin I-Hsuan , Ko Nai-Ying , Huang Yu-Te , Chen Mu-Hong , Lu Wei-Hsin , Yen Cheng-Fang
Published: September 2019

Taiwan held voter-initiated referendums to determine same-sex marriage legalization on 24 November 2018. This study aims to compare suicidal ideation rates in heterosexual and nonheterosexual participants of a first-wave survey (Wave 1, 23 months before the same-sex marriage referendums) and a second-wave survey (Wave 2, one week after the same-sex marriage referendums) in Taiwan and to examine the influence of gender, age, and sexual orientation on the change in suicidal ideation rates in nonheterosexual participants. In total, 3286 participants in Wave 1 and 1370 participants in Wave 2 were recruited through a Facebook advertisement. Each participant completed an online questionnaire assessing suicidal ideation. The proportions of heterosexual and nonheterosexual participants with suicidal ideation were compared between the Wave 1 and Wave 2 surveys. Suicidal ideation rates between participants in the Wave 1 and Wave 2 surveys were further compared by stratifying nonheterosexual participants according to gender, age, and sexual orientation. Nonheterosexual participants in the Wave 2 survey had a higher suicidal ideation rate than those in the Wave 1 survey, whereas no difference was observed in suicidal ideation rates between heterosexual participants in Wave 2 and Wave 1. Nonheterosexual participants who were female, younger, gay, lesbian, and bisexual in Wave 2 had a higher suicidal ideation rate than those in Wave 1. The suicidal ideation rate significantly increased in nonheterosexual participants experiencing the same-sex marriage referendums in Taiwan. Whether civil rights of sexual minority individuals can be determined through referendums should be evaluated.
Currency Market Efficiency Revisited: Evidence from Korea
Authors: Kang Min-woo
Published: September 2019

This study aims to test the efficiency of the Korean foreign exchange market and examine its determinants through several well-established methodologies based on the forward rate unbiasedness hypothesis and covered interest rate parity. The empirical findings indicate that the currency market and its related derivatives markets seem to be inefficient during the 2006–2016 period, but have improved considerably after the 2008 global financial crisis. Further, as the main culprits of market inefficiency, we stress the presence of risk premia in the international financial market and the role of central bank intervention.
The miRNA–mRNA Networks Involving Abnormal Energy and Hormone Metabolisms Restrict Tillering in a Wheat Mutant dmc
Authors: An Junhang , Niu Hao , Ni Yongjing , Jiang Yumei , Zheng Yongxing , He Ruishi , Li Junchang , Jiao Zhixin , Zhang Jing , Li Huijuan , Li Qiaoyun , Niu Jishan
Published: September 2019

Tillers not only determine plant architecture but also influence crop yield. To explore the miRNA regulatory network restraining tiller development in a dwarf-monoculm wheat mutant (dmc) derived from Guomai 301 (wild type, WT), we employed miRNome and transcriptome integrative analysis, real-time qRT-PCR, histochemistry, and determinations of the key metabolites and photosynthesis parameters. A total of 91 differentially expressed miRNAs (DEMs) were identified between dmc and WT. Among them, 40 key DEMs targeted 45 differentially expressed genes (DEGs) including the key DEGs encode growth-regulating factors (GRF), auxin response factors (ARF), and other proteins involved in the metabolisms of hormones and carbohydrates, etc. Compared with WT, both the chlorophyll contents and the photosynthesis rate were lower in dmc. The contents of glucose, sucrose, fructose, and maltose were lower in dmc. The contents of auxin (IAA) and zeatin (ZA) were significantly lower, but gibberellin (GA) was significantly higher in the tiller tissues of dmc. This research demonstrated that the DEMs regulating hormone and carbohydrate metabolisms were important causes for dmc to not tiller. A primary miRNA–mRNA regulatory model for dmc tillering was established. The lower photosynthesis rate, insufficient energy, and abnormal hormone metabolisms restrict tillering in dmc.
Protective Effects and Mechanisms of Vaccarin on Vascular Endothelial Dysfunction in Diabetic Angiopathy
Authors: Xu Fei , Liu Yixiao , Zhu Xuexue , Li Shuangshuang , Shi Xuelin , Li Zhongjie , Ai Min , Sun Jiangnan , Hou Bao , Cai Weiwei , Sun Haijian , Ni Lulu , Zhou Yuetao , Qiu Liying
Published: September 2019

Cardiovascular complications are a major leading cause of mortality in patients suffering from type 2 diabetes mellitus (T2DM). Vascular endothelial dysfunction is a core pathophysiological event in the early stage of T2DM and eventually leads to cardiovascular disease. Vaccarin (VAC), an active flavonoid glycoside extracted from vaccariae semen, exhibits extensive biological activities including vascular endothelial cell protection effects. However, little is known about whether VAC is involved in endothelial dysfunction regulation under high glucose (HG) or hyperglycemia conditions. Here, in an in vivo study, we found that VAC attenuated increased blood glucose, increased glucose and insulin tolerance, relieved the disorder of lipid metabolism and oxidative stress, and improved endothelium-dependent vasorelaxation in STZ/HFD-induced T2DM mice. Furthermore, in cultured human microvascular endothelial cell-1 (HMEC-1) cells, we showed that pretreatment with VAC dose-dependently increased nitric oxide (NO) generation and the phosphorylation of eNOS under HG conditions. Mechanistically, VAC-treated HMEC-1 cells exhibited higher AMPK phosphorylation, which was attenuated by HG stimulation. Moreover, HG-triggered miRNA-34a upregulation was inhibited by VAC pretreatment, which is in accordance with pretreatment with AMPK inhibitor compound C (CC). In addition, both reactive oxygen species (ROS) scavenger N-acetyl-L-cysteine (NAC) and VAC abolished HG-evoked dephosphorylation of AMPK and eNOS, increased miRNA-34a expression, and decreased NO production. These results suggest that VAC impedes HG-induced endothelial dysfunction via inhibition of the ROS/AMPK/miRNA-34a/eNOS signaling cascade.
Roles of the INO80 and SWR1 Chromatin Remodeling Complexes in Plants
Authors: Wang Jianhao , Gao Sujuan , Peng Xiuling , Wu Keqiang , Yang Songguang
Published: September 2019

Eukaryotic genes are packed into a dynamic but stable nucleoprotein structure called chromatin. Chromatin-remodeling and modifying complexes generate a dynamic chromatin environment that ensures appropriate DNA processing and metabolism in various processes such as gene expression, as well as DNA replication, repair, and recombination. The INO80 and SWR1 chromatin remodeling complexes (INO80-c and SWR1-c) are ATP-dependent complexes that modulate the incorporation of the histone variant H2A.Z into nucleosomes, which is a critical step in eukaryotic gene regulation. Although SWR1-c has been identified in plants, plant INO80-c has not been successfully isolated and characterized. In this review, we will focus on the functions of the SWR1-c and putative INO80-c (SWR1/INO80-c) multi-subunits and multifunctional complexes in Arabidopsis thaliana. We will describe the subunit compositions of the SWR1/INO80-c and the recent findings from the standpoint of each subunit and discuss their involvement in regulating development and environmental responses in Arabidopsis.
Identification and Expression Analysis of GRAS Transcription Factors to Elucidate Candidate Genes Related to Stolons, Fruit Ripening and Abiotic Stresses in Woodland Strawberry (Fragaria vesca)
Authors: Chen Hong , Li Huihui , Lu Xiaoqing , Chen Longzheng , Liu Jing , Wu Han
Published: September 2019

The cultivated strawberry (Fragaria × ananassa), an allo-octoploid with non-climacteric fleshy fruits, is a popular Rosaceae horticultural crop worldwide that is mainly propagated via stolons during cultivation. Woodland strawberry (Fragaria vesca), one of the four diploid progenitor species of cultivated strawberry, is widely used as a model plant in the study of Rosaceae fruit trees, non-climacteric fruits and stolons. One GRAS transcription factor has been shown to regulate stolon formation; the other GRAS proteins in woodland strawberry remain unknown. In this study, we identified 54 FveGRAS proteins in woodland strawberry, and divided them into 14 subfamilies. Conserved motif analysis revealed that the motif composition of FveGRAS proteins was conserved within each subfamily, but diverged widely among subfamilies. We found 56 orthologous pairs of GRAS proteins between woodland strawberry and Arabidopsis thaliana, 47 orthologous pairs between woodland strawberry and rice and 92 paralogous pairs within woodland strawberry. The expression patterns of FveGRAS genes in various organs and tissues, and changes therein under cold, heat and GA3 treatments, were characterized using transcriptomic analysis. The results showed that 34 FveGRAS genes were expressed with different degrees in at least four organs, including stolons; only a few genes displayed organ-specific expression. The expression levels of 16 genes decreased, while that of four genes increased during fruit ripening; FveGRAS54 showed the largest increase in expression. Under cold, heat and GA3 treatments, around half of the FveGRAS genes displayed increased or decreased expression to some extent, suggesting differing functions of these FveGRAS genes in the responses to cold, heat and GAs. This study provides insight into the potential functions of FveGRAS genes in woodland strawberry. A few FveGRAS genes were identified as candidate genes for further study, in terms of their functions in stolon formation, fruit ripening and abiotic stresses.
Kinetics of Flavoenzyme-Catalyzed Reduction of Tirapazamine Derivatives: Implications for Their Prooxidant Cytotoxicity
Authors: Nemeikaitė-Čėnienė Aušra , Šarlauskas Jonas , Jonušienė Violeta , Marozienė Audronė , Misevičienė Lina , Yantsevich Aliaksei V. , Čėnas Narimantas
Published: September 2019

Derivatives of tirapazamine and other heteroaromatic N-oxides (ArN→O) exhibit promising antibacterial, antiprotozoal, and tumoricidal activities. Their action is typically attributed to bioreductive activation and free radical generation. In this work, we aimed to clarify the mechanism(s) of aerobic mammalian cell cytotoxicity of ArN→O performing the parallel studies of their reactions with NADPH:cytochrome P-450 reductase (P-450R), adrenodoxin reductase/adrenodoxin (ADR/ADX), and NAD(P)H:quinone oxidoreductase (NQO1); we found that in P-450R and ADR/ADX-catalyzed single-electron reduction, the reactivity of ArN→O (n = 9) increased with their single-electron reduction midpoint potential (E17), and correlated with the reactivity of quinones. NQO1 reduced ArN→O at low rates with concomitant superoxide production. The cytotoxicity of ArN→O in murine hepatoma MH22a and human colon adenocarcinoma HCT-116 cells increased with their E17, being systematically higher than that of quinones. The cytotoxicity of both groups of compounds was prooxidant. Inhibitor of NQO1, dicoumarol, and inhibitors of cytochromes P-450 α-naphthoflavone, isoniazid and miconazole statistically significantly (p < 0.02) decreased the toxicity of ArN→O, and potentiated the cytotoxicity of quinones. One may conclude that in spite of similar enzymatic redox cycling rates, the cytotoxicity of ArN→O is higher than that of quinones. This is partly attributed to ArN→O activation by NQO1 and cytochromes P-450. A possible additional factor in the aerobic cytotoxicity of ArN→O is their reductive activation in oxygen-poor cell compartments, leading to the formation of DNA-damaging species similar to those forming under hypoxia.
Copy-Move Forgery Detection and Localization Using a Generative Adversarial Network and Convolutional Neural-Network
Authors: Abdalla Younis , Iqbal M. Tariq , Shehata Mohamed
Published: September 2019

The problem of forged images has become a global phenomenon that is spreading mainly through social media. New technologies have provided both the means and the support for this phenomenon, but they are also enabling a targeted response to overcome it. Deep convolution learning algorithms are one such solution. These have been shown to be highly effective in dealing with image forgery derived from generative adversarial networks (GANs). In this type of algorithm, the image is altered such that it appears identical to the original image and is nearly undetectable to the unaided human eye as a forgery. The present paper investigates copy-move forgery detection using a fusion processing model comprising a deep convolutional model and an adversarial model. Four datasets are used. Our results indicate a significantly high detection accuracy performance (~95%) exhibited by the deep learning CNN and discriminator forgery detectors. Consequently, an end-to-end trainable deep neural network approach to forgery detection appears to be the optimal strategy. The network is developed based on two-branch architecture and a fusion module. The two branches are used to localize and identify copy-move forgery regions through CNN and GAN.
Translation Quality and Error Recognition in Professional Neural Machine Translation Post-Editing
Authors: Vardaro Jennifer , Schaeffer Moritz , Hansen-Schirra Silvia
Published: September 2019

This study aims to analyse how translation experts from the German department of the European Commission’s Directorate-General for Translation (DGT) identify and correct different error categories in neural machine translated texts (NMT) and their post-edited versions (NMTPE). The term translation expert encompasses translator, post-editor as well as revisor. Even though we focus on neural machine-translated segments, translator and post-editor are used synonymously because of the combined workflow using CAT-Tools as well as machine translation. Only the distinction between post-editor, which refers to a DGT translation expert correcting the neural machine translation output, and revisor, which refers to a DGT translation expert correcting the post-edited version of the neural machine translation output, is important and made clear whenever relevant. Using an automatic error annotation tool and the more fine-grained manual error annotation framework to identify characteristic error categories in the DGT texts, a corpus analysis revealed that quality assurance measures by post-editors and revisors of the DGT are most often necessary for lexical errors. More specifically, the corpus analysis showed that, if post-editors correct mistranslations, terminology or stylistic errors in an NMT sentence, revisors are likely to correct the same error type in the same post-edited sentence, suggesting that the DGT experts were being primed by the NMT output. Subsequently, we designed a controlled eye-tracking and key-logging experiment to compare participants’ eye movements for test sentences containing the three identified error categories (mistranslations, terminology or stylistic errors) and for control sentences without errors. We examined the three error types’ effect on early (first fixation durations, first pass durations) and late eye movement measures (e.g., total reading time and regression path durations). Linear mixed-effects regression models predict what kind of behaviour of the DGT experts is associated with the correction of different error types during the post-editing process.
Therapy of Patients with Neuroendocrine Neoplasia—Evidence-Based Approaches and New Horizons
Authors: Bundschuh Ralph A. , Habacha Bilel , Lütje Susanne , Essler Markus
Published: September 2019

Neuroendocrine tumors (NETs) show low but increasing incidence and originate in multiple organs, including the pancreas, midgut, caecum, rectum, appendix, colon, and lungs. Due to their stunning genetic, histological, and clinical variability, diagnosis and treatment of NETs are challenging. In addition, low incidence and high variability hamper the implementation of high evidence trials. Therefore, guidelines do not cover the complexity of NETs and, frequently, treatment decisions are taken by interdisciplinary tumor conferences at comprehensive cancer centers. Treatment aims are (i) control of tumor growth, (ii) symptom control, as well as (iii) the improvement of progression-free survival (PFS) and overall survival (OS). Here, we discuss high evidence trials facilitating the achievement of these treatment aims. The majority of the evidence exists for treatment with somatostatin analogue, everolimus, peptide receptor radionuclide therapy (PRRT) with 177Lu-DOTATATE, sunitinib, and telotristat. Among those, PRRT is the only treatment option that has the potential to control symptoms, stop tumor growth, and to improve PFS and OS. In contrast, only a low level of evidence exists for treatment with cytotoxic drugs such as streptozotocin and doxorubicine. Finally, we discuss novel treatment options by a combination of cytotoxic drugs, 177Lu-DOTATATE, and tyrosine kinase inhibitors to be tested in randomized prospective trials in the future. In addition, the application of innovative isotopes, such as 225Ac, for PRRT is discussed.
Virus-Induced Asthma/Wheeze in Preschool Children: Longitudinal Assessment of Airflow Limitation Using Impulse Oscillometry
Authors: Konstantinou George , Papadopoulos Nikolaos , Manousakis Emmanouel , Xepapadaki Paraskevi
Published: September 2019

Several researchers have assessed the utility of Impulse Oscillometry System (IOS) in diagnosing and evaluating the severity of respiratory diseases in childhood, but none has investigated the impact of the fluctuations of IOS parameters in an individualized manner. In this two-year prospective study, we aimed to longitudinally evaluate changes in airflow limitation and bronchodilator responsiveness in steroid-naïve four- to six-year-old children during a virus-induced wheezing episode, with IOS pulmonary resistance parameters set at 5 (R5) and 20 (R20) Hz. Moreover, feasibility and reproducibility, in addition to the diagnostic properties of these parameters were examined. Lung function was assessed every six weeks (baseline), within the first 48 h following an acute wheezing episode (Day 0), after 10, and after 30 days. Forty-three out of 93 recruited children (4.5 ± 0.4 years old) experienced a wheezing episode during the study period. All children were able to perform the IOS effort in an acceptable and highly reproducible manner. R5 and R20 fluctuated independently of atopy, age, height, and weight. On Day 0, R5 values were significantly lower than the respective baseline values and returned to individual baseline levels within 10 days. Post-bronchodilation R5 values were similar to the baseline ones, reflecting a reversible airway obstruction on Day 0. Response to bronchodilation (ΔR5) was significantly more pronounced on Day 0. ΔR5 values lower than −20.5% had a sensitivity of 70% and a specificity of 76% and could accurately identify up to 75% of the examined preschoolers. This study provides evidence in favor of the objective utility of IOS as an easy, highly reproducible, and sensitive technique to assess clinically significant fluctuations and bronchodilation responses suggestive of airflow limitation. Reference values although necessary are suboptimal, utilizing the personal best values as personal reference is useful and reliable.
The Naked Mole-Rat: An Unusual Organism with an Unexpected Latent Potential for Increased Intelligence?
Authors: Schulze-Makuch Dirk
Published: September 2019

Naked mole-rats are eusocial, hairless mammals that are uniquely adapted to their harsh, low-oxygen subsurface habitat. Although their encephalization quotient, a controversial marker of intelligence, is low, they exhibit many features considered tell-tale signs of highly intelligent species on our planet including longevity, plasticity, social cohesion and interaction, rudimentary language, sustainable farming abilities, and maintaining sanitary conditions in their self-built complex housing structures. It is difficult to envision how naked mole-rats would reach even higher levels of intelligence in their natural sensory-challenged habitat, but such an evolutionary path cannot be excluded if they would expand their range onto the earth’s surface.
Study of the Structure and Antimicrobial Activity of Ca-Deficient Ceramics on Chlorhexidine Nanoclay Substrate
Authors: Pazourková Lenka , Reli Magda , Hundáková Marianna , Pazdziora Erich , Predoi Daniela , Simha Martynková Gražyna , Lafdi Khalid
Published: September 2019

Novel biomedical composites, based on organically modified vermiculite and montmorillonite with deposited Ca-deficient hydroxyapatite (CDH), were prepared. The monoionic sodium forms of vermiculite and montmorillonite were intercalated with chlorhexidine diacetate (CA). The surfaces of organoclays were used for the precipitation of Ca-deficient hydroxyapatite. The composites with Ca-deficient hydroxyapatite showed very good antibacterial effects, similar to the antimicrobial activity of pure organoclay samples. Better antibacterial activity was shown in the organically modified montmorillonite sample with Ca-deficient hydroxyapatite compared with the vermiculite composite, but, in the case of Staphylococcus aureus, both composites showed the same minimum inhibitory concentration (MIC) value. The antimicrobial effect of composites against bacteria and fungi increased with the time of exposure. The structural characterization of all the prepared materials, performed using X-ray diffraction and FT infrared spectroscopy analysis, detected no changes in the original clay or CDH during the intercalation or precipitation process, therefore we expect the strength of the compounds to be in the original power.