bluuebunny/crossref_metadata_2025 huggingface dataset:
Care and Loyalty in the Closet: A review of scholarship on sexual identity disclosure in queer South Asian women
Authors: Marwaha Pooja
Published: May 2023

This paper highlights a gap in scholarship on personal and family relationships of British queer South Asian women and queer women of colour. It enables fields of study within queer theory, family sociology and intimate relationships to move beyond Western normative frames when looking at queer individuals  and their relationships. Existing research on queer people of colour is limited and, where present, rarely focuses on diasporic British queer South Asian  women. This paper looks at the diasporic context and highlights why the South Asian family unit should not be categorised as homophobic. It looks at how notions of ‘coming out’ and ‘the closet’ are Westernised and Eurocentric. It also looks at the use of ‘the closet’ as a shield emanating from a place of care for the family and self. This paper also presents queer women of colour as individuals who are moving beyond a binary of being queer and ostracised from family or  as pretending to be straight to remain in their family, asking if this can also be the case for queer South Asians. Finally, throughout this paper, the discussion  touches on the concept of ‘chosen families’ and how it may apply to queer South Asian women.
AUDIT COMMITTEE CHARACTERISTICS AND VOLUNTARY DISCLOSURE (STUDY ON MANUFACTURING COMPANIES IN 2017-2019)
Authors: Safitri Nurul Aisyah  , Isna Putri Rahmawati
Published: May 2023

This study centers on how the audit committee's characteristics impact voluntary disclosure. The composition of the audit committee is shown by its size, percentage of independent commissioners, and gender diversity. This study used 255 observations over 3 years, from 2017 to 2019, and samples from industrial firms recorded on the Indonesia Stock Exchange. In this study, a multiple linear analysis is conducted. The results show that the audit committee size has a negative effect on voluntary disclosure. The ratio of independent commissioners and the gender diversity of the audit committee have a positive impact on voluntary disclosure.
THE EFFECT OF GOVERNMENT ACCOUNTING STANDARD APPLICATION ON THE QUALITY OF FINANCIAL STATEMENTS (STUDY ON REGIONAL APPARATUS ORGANIZATIONS OF KARANGANYAR REGENCY)
Authors: Galuh Syarifah Mentari Dahana  , Purnomowati Nasyiah Hasanah
Published: May 2023

This research aims to analyze the effect of the implementation of the government accounting standard (Standar Akuntansi Pemerintahan/SAP) on the quality of the financial statements through the study of perceptions of the financial management staff and to analyze the extent to which the government’s internal control system (Sistem Pengendalian Intern Pemerintah/SPIP) strengthens the relationship between government accounting standard implementation and the quality of the financial statements. The samples were taken using the saturated sampling technique. A total of 171 respondents from 57 regional apparatus organizations (Organisasi Perangkat Daerah/OPD) in Karanganyar Regency were invited to participate in this study, with 117 data used for analysis. The data were analyzed using a simple regression test and interaction test. The results of the study show that the implementation of government accounting standards gives a positive effect on the quality of financial statements.
Division of Labour in Coastal Community: The Equity of Role-Play Between Bugis Women and Men in Kupang
Authors: Syahrul Syahrul , Zahrawati Fawziah , Nursaptini Nursaptini
Published: May 2023

The study aims to know the division of labor between women and men in the Bugis Families at Kupang coastal community. Collecting the data through Focus Group Discussion (FGD) with 20 familial members such as wives and husbands, and gathering in-deep information by conducting interviews and observations. The result of this study shows that: (1) In Bugis families, wife and husband work representing social solidarity because there is no role play that the only wife should cook, clean, and wash, while several husbands stay at home for cooking, washing, and nursing. (2) Gender division of labor approach to highlight the role, benefits, and risks of women and men in the coastal area. Women and men supply seaweed farming as a family business, and both of them work with a division of labor. The wife used the extra money for daily expenses, whereas the husband used their extra money for bigger investments. (3) Both women and men work not only equal but also equity because fishing is dangerous for women, but men can do it, while fishery multi-products are difficult for Bugis men, but it easy for women. 
Work-Life Balance of Regional Midwife During the Covid-19 Pandemic Time
Authors: Muafiah Evi , Sofiana Neng Eri
Published: May 2023

Perempuan pekerja pada masa kini mulai banyak, begitu pula pada profesi dalam bidang seperti seorang bidan yang di Indonesia hanya menilai seorang perempuan saja.Seorang bidan yang bekerja di puskesmas dan membuka praktik sendiri di rumah sangat rentan terhadap virus. Wabah COVID-19 yang telah hidup bersama masyarakat selama hampir tiga tahun ini menjadi ancaman nyata bagi setiap orang, khususnya tenaga kesehatan. Apa saja bidan tersebut juga merupakan bidan wilayah yang memiliki tanggung jawab kesehatan satu wilayah desa atau kelurahan. Selain beban, sebagai seorang perempuan yang selalu dilekatkan dengan pekerjaan domestik menjadikkannya beban ganda. Tulisan ini akan melihat bagaimana seorang bidan wilayah menyeimbangkan dan menganalisis terhadap WLB (Work-Life Balance).Penelitian ini dilakukan dengan metode kualitatif dengan data yang diperoleh dari hasil observasi, wawancara, dan dokumentasi. Hasilnya, seorang bidan wilayah dapat mencapai keseimbangan kehidupan-kerja atau WLB (Work-Life Balance) sehingga mampu memenuhi faktor-faktor WLB seperti adanya keseimbangan waktu, keterlibatan, dan kepuasan. Dalam tulisan, bidan wilayah yang menjadi objek kajian mampu mencapai WLB sebab mampu mengatasi faktor-faktor tersebut.
Environmental Literacy Investigation Biology Education Students at Islamic Colleges Based on Gender and Parents' Occupation in Jambi Province
Authors: Zb Aminah Aminah , Harisah Harisah , Iskandar Iskandar , Sulman Fauzan Fauzan
Published: May 2023

Eivironmental literacy is an important aspect of individual abilities in maintaining environmental conditions. This study aimed to determine the understanding of environmental literacy of Biology Tadris students with Islamic religious college backgrounds based on Gender and Parents’ Occupation. This study uses a descriptive quantitative approach. The research population is Biology Education students from Islamic Religious College in Jambi Province. The research sample was determined by purposive sampling, which consisted of 118 people. The study results are; students’ environmental literacy in general is quite good which female students are in good criteria with slightly better than boys. Then in terms of the work of parents, the criteria of good enough which the highest average score was obtained by farmers. Environmental knowledge and student cognitive skills obtained from learning and information obtained from the environment. The ability of environmental literacy based on gender has become a must to be observed as a form of consistency and seriousness in understanding the differences and the need to increase the understanding of environmental literacy that students own.
Анализ динамики эпидемического процесса COVID-19 в мире за неделю с 29.04.2023 г. по 05.05.2023 г.
Authors: Зимирова Анастасия , Поспелов Михаил , Иванова Александра , Дмитриева Людмила , Чумачкова Елена , Карнаухов Игорь , Щербакова Светлана , Кутырев Владимир
Published: May 2023

В обзоре представлен анализ эпидемиологической ситуации в странах мира по COVID-19 за неделю с 29.04.2023 г. по 05.05.2023 г.
Financial leverage and ASEAN corporate governance scorecard on accounting conservatism
Authors: Hariyani Diyah Santi , Agustine Afrida Dwi , Wahyuandari Wenni , Minarni Eni
Published: May 2023

This study aims to examine the effect of financial leverage and the ASEAN Corporate Governance Scorecard (ACGS) on accounting conservatism.  This study uses a quantitative descriptive research approach to test hypotheses. The population includes companies that are on the Kompas 100 stock index. The sampling method used is purposive sampling, which is a company that is in the top rank 50 of the ASEAN Corporate Governance Scorecard for the period 2017-20 20. After the data was selected, there were 40 companies selected according to predetermined criteria. The analysis method uses multiple linear regression with SPSS 23. The results of this study show that Financial Leverage has a significant effect on Accounting Conservatism and the ASEAN Corporate Governance Scorecard has a significant effect on Accounting Conservatism. The results of this study are in line with the phenomenon in Indonesia that 40 companies that have been included in the ASEAN Top 50 were awarded the ACGS, this shows that corporate governance in Indonesia is still weak. The results of this study do not support the agency theory and the theory of the firm. The results of this study can be used by the OJK in improving the standards of good corporate governance.
ELM (Elaboration Likelihood Model) paradigm in snack SMEs: Experimental study
Authors: Surjadi Calvin Cliff , Teofilus Teofilus , Gosal Gladys Greselda , Setiobudi Auditia , Mudzakkir Mohammad Fakhruddin
Published: May 2023

Penelitian ini bertujuan untuk mengetahui apakah kualitas informasi, keragaman produk, dan kemasan berpengaruh secara signifikan terhadap minat beli konsumen pada UMKM di Surabaya yang bergerak di sektor makanan ringan. Penelitian ini menggunakan teori Elaboration Likelihod Model untuk membantu menganalisis bagaimana seseorang memproses informasi yang nantinya dapat membantu meningkatkan minat beli. Penelitian ini menggunakan metode penelitian eksperimental, dengan sampel yang berjumlah 270 mahasiswa Universitas Ciputra sebagai responden yang dibagi menjadi 9 kelompok dengan kondisi eksperimen yang berbeda-beda. Teknik sampling menggunakan probability sampling, dan proses analisis data dilakukan dengan menggunakan alat analisis SPSS dan JASP. Hasil peneltian ini menemukan bahwa ketiga variabel tersebut secara individu memiliki pengaruh yang signifikan terhadap minat beli, tetapi penemuan berikutnya menunjukkan bahwa ketiga variabel tersebut secara bersamaan tidak memiliki pengaruh yang signifikan terhadap minat beli, kecuali untuk variabel yang mewakili rute periferal yaitu keragaman produk dan kemasan. Berdasarkan hasil penemuan ini, disimpulkan bahwa saat konsumen berminat untuk membeli suatu produk, mereka akan cenderung untuk memperhatikan satu hal saja, yaitu antara melalui rute sentral atau rute periferal.
Menilai pertumbuhan dan trend pada studi sustainable development goals: Analisis bibliometrik
Authors: Setiyowati Supami Wahyu , Fauzan Fauzan , Indiraswari Susmita Dian
Published: May 2023

Produksi ilmiah tentang Sustainable Development Goals telah tumbuh dalam beberapa tahun terakhir. Namun, masih sedikit penelitian yang memberikan gambaran global tentang topik ini. Tujuan penelitian adalah untuk mengidentifikasi tren penelitian serta produksi ilmiah dari waktu ke waktu. Analisis bibliometri digunakan untuk menganalisis sampel dari 3608 artikel yang diterbitkan antara tahun 1996 hingga 2022. Sustainable Development Goals merupakan kueri yang digunakan dalam judul artikel untuk mendapatkan metadata dari database bibliografi Scopus. Microsoft excel digunakan untuk melakukan analisis frekuensi, VOSviewer untuk visualisasi data dan Harzing's Publish or Perish digunakan untuk metrik kutipan. Hasil menunjukkan bahwa ada peningkatan literatur tentang Sustainable Development Goals dari tahun 1996 hingga 2022, dan peningkatan yang signifikan sejak tahun 2016. Ada berbagai penelitian yang melibatkan kolaborasi multi-penulis yang diterbitkan dalam berbagai bahasa, dengan total 32 penulis dari 141 negara yang berbeda dan 32 lembaga. Amerika Serikat adalah kontributor terbesar untuk penelitian ini. Publikasi terbanyak diterbitkan oleh Sustainability Switzerland. Temuan ini dapat membantu penelitian di masa depan terkait dengan ikhtisar penelitian Sustainable Development Goals di seluruh dunia dari waktu ke waktu.
Application of Erythrocyte Indices for Screening Thalassemia Trait in Jiujiang prefecture
Authors: ke maichun , Yang Yanping , Li Wei , Dong Xiaoheng , Wang Qing , Tong Lu
Published: May 2023

Background: The screening efficiency of Erythrocyte indices varies in different regions. This study aimed to analyze the application efficiency of 11 Erythrocyte indices in Jiujiang prefecture.
Methods: A total of 2379 patients with suspected thalassanemia were enrolled in Jiujiang Maternal and Child Health Hospital. Thalassanemia genes were detected by reverse dot blot hybridization. In addition, receiver operating characteristic curve (ROC) was used to analyze and calculate the sensitivity, specificity, Youden's Index(YI), positive likelihood ratio(+LR) , negative likelihood ratio(-LR) and area under curve (AUC) of the Erythrocyte indices.
Results: 1)Thalassanemia carriers in Jiujiang prefecture mainly involved α- thalassanemia and β-thalassanemia, with the detection rate of 11.60%%. Thereinto, --SEA/αα(50.35%%) and -α3.7/αα(39.86%%) predominated in α-Thalassanemia, while the commonest gene mutation types of β-Thalassanemia were βIVS-II-654/βN, βCD41-42/βN, and βCD17/βN, accounting for 64.29%,15.87% and 9.542%, respectively. 2) Of 11 erythrocyte indices, Green and King (G&K) exhibited the highest diagnostic efficiency, with the AUC above 90%, a high sensitivity and a good specificity, which followed by England and Fraser (E&F), Sirdah(SI), Jayabose (RDW index) and Ricerca(RI). while Bessman), Ehsani(EI), Srivastava(Sriv), Shine and Lal (S&L), MentzIer(MI) and RBC count(in million) possessed a lower application value for thalassanemia screening.
Conclusion: 1)Analysis of thalassanemia genotypes can provide a theoretical basis for thalassanemia screening, prenatal diagnosis and genetic counseling. 2)Compared with other erythrocyte indices, Green and King (G&K) showed higher application value for thalassanemia screening, with the largest AUC above 90%. which is considered feasible for thalassanemia screening in Jiujiang prefecture, Jiangxi province.
Effectiveness of Neuromodulation on Abductor Muscles Electrical Activity in Subjects With Low Back Pain: A Randomized, Controlled Crossover Trial
Authors: Benítez-Martínez Josep C. , García-Haba Beatriz , Fernández-Carnero Samuel , Pecos-Martin Daniel , Sanchez-Romero Eleuterio A. , Selva-Sarzo Francisco , Zaldivar Juan Nicolás Cuenca-
Published: May 2023

Introduction
Non-specific chronic low back pain (NSCLBP) is one of the main reasons of loss of function, that can have consequences such as job absenteeism and a decrease in the quality of life. Based in clinical findings and scientific studies, different risks factors have been stated as potential implication, such as muscles weakness and muscle tightness. Electromagnetic fields positively influence human tissue and have several therapeutic effects such as: pain relief, healing bone fracture, myorelaxation, myostimulation and joint mobilization. The aim of this study was to investigate whether the effect of a magnetic particle tape applied to the lumbar area in subjects with NSCLBP influences the strength of abductor muscle contraction and whether an immediate effect is obtained on surface electromyography (sEMG) of the Gluteus Medius and the Tensor of the Fascia Lata.
Methods
It was carried out a double-blind, randomized, controlled, crossover trial and with test retest, with 41 consecutive patients younger than 65 years who previously diagnosed with NSCLBP to assess the effect of a tape with magnetic particles over hip abductor muscles strength and activity. Electromyographic (EMG) and force data were obtained during the Hip Stability Isometric Test (HipSIT). The HipSIT was used to assess the abduction strength using a hand-held dynamometer and electromyography (EMG). The HipSIT uses the maximum voluntary isometric contraction (MVIC). Four trials were recorded and the mean extracted for analysis. The tape was applied with either a magnetic particle tape or a sham magnetic particle tape bilaterally without tension on from L1 to L5 paravertebral muscles.
Results
The significant increase in the recruitment of fibers and the significant increase in the maximum voluntary contraction by applying magnetic particle tape with respect to the placebo tape, correspond to the increases in the Peak Force and the decrease in the time to reach the maximum force (peak time) of both muscles.
Conclusions
Application of a magnetic particle tape in people with low back pain suggest an increase in muscle strength of the Gluteus Medius and Tensor Fascia Lata bilaterally during the HipSIT test. Lumbar metameric neuromodulation with Magnetic Tape improves muscle activation of the hip musculature.
Impact Statement
The findings of this study will provide data on the effectiveness of a tape with magnetic particles for People with NSCLP for health care policy makers, physicians, and insurers. Data from this study will also inform future pragmatic trials for non-pharmacological interventions and chronic musculoskeletal pain conditions.
First Investigation of Thermophilic and Pathogenic Campylobacter Species in Drinking Water Sources in Turkey
Authors: Ö Müjdat , ŞUMNULU Deniz , ŞEN Ece
Published: May 2023

This study aims to determine for the first time the presence of thermophilic Campylobacter (C. jejuni, C.coli, and C. lari) in drinking water sampled from Edirne city center, districts and rural areas (villages, towns, and private enterprises) located near the Bulgarian and Greece border regions of Western Turkey. We also aimed to determine the variations in the distribution of thermophilic Campylobacter sp. at different seasons and water sources. For this purpose, Escherichia coli, which is a definite indicator of fecal pollution, was investigated and enumerated by using the membrane filtration method according to ISO 9308-1: 2014 Standard. qPCR analyses of thermophilic Campylobacter species were performed by using two different commercial kits in E. coli detected samples. In our study, E. coli was detected in 67 out of 1644 water samples taken from 455 different stations. It was observed that all of the samples contaminated with E. coli were groundwater samples, which were not subjected to any treatment process. Only one E.coli contaminated sample had a low chlorine level of 0.03 ppm and others were free of chlorine. Thermophilic Campylobacter (C. jejuni, C. coli, and C. lari) species were detected in 32 out of 67 water samples by multiplex qPCR analysis by using Mericon Campylobacter Triple Kit. However, since this kit can only reveal the genus and can not determine the species of this bacterium, C. jejuni Standard Kit was used to detect the C. jejuni which is the most likely species to be seen. qPCR analyses by using the C. jejuni Standard Kit demonstrated that 5 of 67 water samples were positive for this bacterium. This study, which was carried out first time in Turkey, showed that thermophilic Campylobacter can be found in drinking water sources in all regions of Edirne, and disruptions in the treatment, storage, and distribution of water may cause possible thermophilic Campylobacter-related cases even possible epidemics in this Western border region.
Future benefits of emission controls in mitigating climate-driven regional transport of PM2.5 in China
Authors: Li Siwei , Wang Shansi , Xing Jia , Ding Yu , Hu Senlin , Liu Shuchang , Qin Yu , Dong Zhaoxin , Dong Jiaxin , Song Ge , Dong Lechao , Fan Chunying , Teng Mengfan , Han Ge , Ma Xin , Lu Jinjun
Published: May 2023

Regional transport is equally significant to local sources in contributing to PM2.5 pollution and associated environmental inequality. In the context of future climate change, the effect of the responses of regional transport to the warming climate and controlling emissions has not been thoroughly investigated. Here we establish cross-province PM2.5 source-receptor matrix in China in 2015 baseline and two climate pathways in 2050s (SSP585 and SSP126), using Community Multi-scale Air Quality model embedded with the Integrated Source Apportionment Method. Results suggest that across-regional transport contributes 36.1 % - 62.3 % of PM2.5 in five severely polluted regions, which is even more important compared to inner transport within the target region (3.8 % - 19.4 %), especially in Chuanyu and Fenwei regions which are polluted by large PM2.5 transport (over 50 %) from outside regions. Such results imply that joint-control policy should not only focus on neighboring provinces. Controlling pollutant emission is undoubtedly reduced the concentration of PM2.5 regional transport (by -5.25 µg/m3 on average), largely exceeding the influence from the meteorological fluctuations (by -0.82 µg/m3 on average) driven by the climate change in 2050s. On the other hand, future controlled pollutant emissions proved that strengthen across-regional transport with an enlarged relative contribution to total PM2.5 concentration (8.46%) in 2050, along with a decreased contribution from local sources (8.54%). Therefore, environmental inequality issue should be pay more attention in the context of the reduction of pollution and carbon emissions.
GeNepher data- and biobank for patients with (suspected) genetic kidney disease: rationale, design and status update
Authors: Claus Laura R. , Zwaag Bert van der , Nguyen Tri Q. , Knoers Nine V.A.M. , de Borst Martin H. , Rookmaker Maarten B. , Lilien Marc R. , Eerde Albertien M. van , Contributors GeNepher Biobank
Published: May 2023

Background Clinical research on genetic kidney disease is thriving and the need for large cohorts, prospective data collection and biobanking is increasing. We aim to create a sustainable large genetic kidney disease biobank with a vast amount of uniformly collected high-quality data that is readily available for future research, with an infrastructure that allows for recontacting participants.Methods The GeNepher data- and biobank is an ongoing data- and sample collection that includes patients and family members with known and/or suspected genetic kidney disease. With a tiered approach participants can give broad consent for including their 1) available medical data (including genetic testing results), 2) inclusion of massively parallel sequencing data for add-on analysis, and 3) additional biobank sampling (e.g. urine for tubuloids, skin biopsy for fibroblasts). Recontacting is possible for additional data collection, novel research opportunities and return of relevant findings.Discussion The GeNepher data- and biobank collects prospective and retrospective data from kidney disease patients and their relatives. The broad consent allows for research that extends beyond one specific research question. Herewith, this biobank aims to 1) increase the scientific knowledge based on disease mechanisms including (novel) monogenic causes, 2) study modifiers, 3) improve care, including reproduction related research questions. Furthermore, it facilitates recontacting for opportunities in treatment development or when diagnose specific trials are started or specific treatment is approved.Conclusion The GeNepher biobank is designed to support a wide range of research projects by providing access to a diverse population of patients with (suspected) genetic kidney disease and has the potential to make a significant contribution to the field of rare kidney disease research.
Thrombin Worsens Extravascular Lung Water and Outcomes of Septic patients with Acute Respiratory Distress Syndrome
Authors: Chung Fu-Tsai , Kuo Chih-Hsi , Wang Chun-Hua , Lin Shu-Min
Published: May 2023

Introduction: Endothelial cell (EC) activation may increase systemic vascular permeability caused an extravasation of water (EVLW) in sepsis with acute respiratory distress syndrome (ARDS). The correlation of thrombin and EVLW in sepsis and ARDS hasn’t addressed.Methods Septic patients with ARDS were prospectively enrolled during 2014 and 2016, and EVLW and serum thrombin on day 1 and 3 were measured and compared between those with survival and non-survival. Additionally, morphologic change of human umbilical vein endothelial cells (HUVECs) with serum from patients with high and low EVLW was conducted.Results Twenty-seven patients were enrolled and the baseline characters including age, gender, APACHE II, prior 24hrs fluid balance, BMI, and shock status were similar between survivors and non-survivors. But day 1 EVLW was higher in non-survivor (27.5 ± 8.4 vs. 22 ± 6.5 ml/kg, p = 0.049). EVLW of survivor was improved from day 1 to day 3 (22 ± 6.5 vs. 11 ± 3.8 ml/kg, p = 0.0001), but it did not improve in non-survivor (27.5 ± 8.4 vs. 28 ± 6.7 ml/kg, p = 0.89). Thrombin level of survivor significantly improved (1.03 ± 0.55 vs. 0.87 ± 0.25 U/ml, p = 0.04) but it did not improve in non-survivor (1.97 ± 0.75 vs. 2.2 ± 0.75 U/ml, p = 0.07) from day 1 to day 3. EVLW and thrombin revealed a positive correlation (r2 = 0.71, p < 0.0001). In vitro, the morphology and junction of HUVECs were changed when serum of patients with high EVLW was added, but those did not change when serum of patients with low EVLW was added.Conclusion The level of EVLW and thrombin correlated positively and may impact the survival of these patients. Thrombin inhibitor may act as a novel valuable approach for preventing endothelial hyper-permeability and provides the rational basis for the use of this kind of drug in patients with severe sepsis and ARDS.
Reinforcing decellularized small intestine submucosa with cellulose acetate nanofibrous and silver nanoparticles as a scaffold for wound healing applications
Authors: Zavareh Zahra Khorasani , Asbagh Reza Akbari , Hajikhani Kiana , Tabasi Asieh Heirani , Nazari Hojjatollah , Abbasi Maryam , Moghaddam Mohammadamir Ghasemian , Behboudi Behanm , Kazemeini Alireza , Tafti Seyed Mohsen Ahmadi
Published: May 2023

Purpose The formation of chronic wounds accounts for considerable costs in health care systems. Despite the several benefits of decellularized small intestinal submucosa (SIS) as an appropriate scaffold for different tissue regeneration, it has shortcomings such as lack of antibacterial features and inappropriate mechanical properties for skin tissue regeneration. We aimed to examine the efficacy and safety of decellularized SIS scaffold enhanced with cellulose acetate (CA) and silver (Ag) nanoparticles (NPs) for healing full-thickness wounds.Methods The scaffolds were prepared by decellularizing bovine SIS and electrospinning CA/Ag nanoparticles and characterized using a transmission electron microscope (TEM), scanning electron microscope (SEM), tensile testing, and X-ray diffraction. In vivo evaluations were performed using full-thickness excisions covered with sterile gauze as the control group, SIS, SIS/CA, and SIS/CA/Ag scaffolds on the dorsum of twenty male Wistar rats divided into four groups randomly with 21-days follow-up. All in vivo specimens underwent Masson's trichrome (MT) staining for evaluation of collagen deposition, transforming growth factor-β (TGF-β) immunohistochemistry (IHC), and Haematoxylin Eosin(H&E) staining. The IHC and MT data were analyzed with the ImageJ tool by measuring the stained area.Results The TEM results revealed that Ag nanoparticles are successfully incorporated into CA nanofibers. Assessment of scaffolds hydrophilicity demonstrated that the contact angle of SIS/CA/Ag scaffold was the lowest. The in vivo results indicated that the SIS/CA/Ag scaffold had the most significant wound closure. H&E staining of the in vivo specimens showed the formation of epidermal layers in the SIS/CA/Ag group on day 21. The percentage of the stained area of MT and TGF-β IHC staining’s was highest in the SIS/CA/Ag group.Conclusion The decellularized SIS/CA/Ag scaffolds provided the most significant wound closure compared to other groups and caused the formation of epidermal layers and can be considered a novel method for enhancement of healing full-thickness wounds. Additionally, the collagen deposition and expression of TGF-β increased significantly in SIS/CA/Ag group.
Association Between Past Hepatitis B Infection and abdominal aorta calcification: National Health and Nutrition Examination Survey
Authors: Zhang kai , Cao Xiaoxiao , Gu Fangming , Gu Zhaoxuan , Han Yu , Hou Zhengyan , Yu Xiaoqi , Cai Tianyi , Gao Yafang , Xie Jinyu , Chen Jianguo , Chen Bowen , Liu Tianzhou
Published: May 2023

Introduction:
With mounting evidence, there is a postulation that Hepatitis B Infection may be a risk factor for atherosclerotic diseases. Abdominal aortic calcification (AAC) has been found to be significantly associated with subclinical atherosclerotic diseases. Our objective was to investigate the relationship between Hepatitis B core antibody and abdominal aortic calcification.
Methods:
In this cross-sectional observational study, data from the National Health and Nutrition Examination Survey (NHANES) conducted during 2013–2014 were utilized to investigate the relationship between Hepatitis B core antibody and AAC. The study used dual-energy X-ray absorptiometry to measure AAC and evaluated it using the Kauppila score system. The variable LBXHBC of the NHANES dataset was reviewed to identify patients with positive Hepatitis B core antibody. To assess the association between Hepatitis B core antibody and AAC, multivariate logistic regression mixed models and subgroup analyses were conducted.
Results:
The 2013–2014 data showed documentation of 288 patients with positive Hepatitis B core antibody. After adjusting for multiple variables, the multivariable odds ratios (95% CI) revealed that severe AAC was correlated with Hepatitis B infection (OR = 1.52, 95%CI [1 ~ 2.3]; p = 0.05), as opposed to the no Hepatitis B group. However, this association was not observed among individuals with mild AAC (OR = 1.22, 95%CI [0.9 ~ 1.65]; p = 0.205). Results were consistent in multiple subgroup and sensitivity analyses.
Conclusions:
Individuals who tested positive for Hepatitis B core antibody had a higher likelihood of severe AAC compared to those who tested negative, while no significant association was observed among individuals with mild AAC.
Universal adaptive optics for microscopy through embedded neural network control
Authors: Booth Martin , Hu Qi , Hailstone Martin , Wang Jingyu , Wincott Matthew , Stoychev Danail , Atilgan Huriye , Gala Dalia , Chaiamarit Tai , Parton Richard , Antonello Jacopo , Packer Adam , Davis Ilan
Published: May 2023

The resolution and contrast of microscope imaging is often affected by aberrations introduced by imperfect optical systems and inhomogeneous refractive structures in specimens. Adaptive optics (AO) compensates these aberrations and restores diffraction limited performance. A wide range of AO solutions have been introduced, often tailored to a specific microscope type or application. Until now, a universal AO solution – one that can be readily transferred between microscope modalities – has not been deployed. We propose versatile and fast aberration correction using a physics-based machine learning assisted wavefront-sensorless AO control (MLAO) method. Unlike previous ML methods, we used a bespoke neural network (NN) architecture, designed using physical understanding of image formation, that was embedded in the control loop of the microscope. The approach means that not only is the resulting NN orders of magnitude simpler than previous NN methods, but the concept is translatable across microscope modalities. We demonstrated the method on a two-photon, a three-photon and a widefield three-dimensional (3D) structured illumination microscope. Results showed that the method outperformed commonly-used model-based sensorless AO methods. We also showed that our ML-based method was robust in a range of challenging imaging conditions, such as extended 3D sample structures, specimen motion, low signal to noise ratio and activity-induced fluorescence fluctuations. Moreover, as the bespoke architecture encapsulated physical understanding of the imaging process, the internal NN configuration was no-longer a “black box”, but provided physical insights on internal workings, which could influence future designs.
Extensive studies on the Electrochemical, Photocatalytic and Antimicrobial Applications of Bio assisted Nano Titania
Authors: N Saikumari , S Sudhakhar K
Published: May 2023

In recent days synthesis and structuring of intelligent nano materials investigated and reported has developed critical scientific ideas to the greater extent. The excellent thermal, optical and electrical properties along with its resistant to corrosion, wear, oxidation and erosion made it unique for sustainable environmental applications. Here nano structured Titanium di oxide particles synthesized from its precursor via template assisted sol-gel technique have been verified as corrosion inhibitor of brass alloy in acid medium along with its photo catalytic and anti-microbial applications. The physico-chemical parameters of the synthesized nano materials were studied using XRD, FT-IR, UV-DRS, SEM, TEM and BET analytical techniques and revealed the impact of tea leaf extract as a template in producing a nano catalyst NTG about 14 nm in size with tailored structural, optical and morphological characteristics. The rate of corrosion of a brass strip in acid medium is verified by weight loss method and the inhibitor efficiency increased with the increase in concentration of the nano catalyst. The catalytic activity is proved against the photo degradation of a toxic melamine, a trimer of cyanamide. The synthesized nano catalyst showed excellent antimicrobial properties proven against the growth of K. pneumonia and H. influenza.
Use of Medication for Preterm Labor: Retrospective Cohort Study in a Single Tertiary Center in Wuhan, China
Authors: Lv Dan , Zhang Yanling , Zhang Xiaolei , Li Xufang , Jallow Fatoumata , Li Fei , Xie Yin , Li Fanfan , He Mengzhou , Fan Yao , Li Wei , Feng Ling , Lin Xingguang , Deng Dongrui
Published: May 2023

Background Prevention and treatment in preterm birth are still under intensive investigation. A gap exists between evidence-based recommendations and clinical practice. A deeper understanding of the prevalence of medication use is an essential step toward improving the care of pregnant women. This study aimed to address this issue.Methods A retrospective cohort was conducted between December 2018 and November 2019 in Tongji Hospital (Wuhan). In total, 878 pregnant women were enrolled. Information on maternal characteristics, medication prescription, maternal outcomes (including post-partum hemorrhage), and neonatal outcomes (including APGAR score, birthweight, neonatal intensive care unit (NICU) admission, cardiopulmonary and neurological diseases) in the linking system were retrieved.Results The overall coverage of the common medications, including antenatal corticosteroids (ACS), magnesium sulfate, tocolytics, and progesterone, was 84.5%, 52.8%, 24.9%, and 13.3%, respectively. The treatment plan varied widely regarding the regimen, dosing, duration, and combination of these drugs. Specifically, tocolytic maintenance, which means the administration duration of more than 48 hours in this study, increased the risk of post-partum hemorrhage; it also significantly increased birthweight, but was not a risk factor of large-for-gestational-age (LGA).Conclusions This retrospective study in a single tertiary center disclosed that obstetric medications for preterm labor are highly individualized and do not strictly conform to current guidelines. This phenomenon is not uncommon in other countries. Clinical practice and evidence-based recommendations need to be carefully balanced; more studies are warranted to provide high-quality evidence to aid clinical decisions.
Behavior diversity in Odonata larvae is increased by water turbidity
Authors: Palacino-Rodríguez Fredy , Silva-Brito Joas , Juen Leandro , Palacino-Penagos Diego Andrés
Published: May 2023

Aquatic insects’ behavior changes due to physiological constraints, trophic interactions, habitat selection, and biotic interactions. Addressing these topics can help to potentiate our understanding of ecosystem services and community structure. Here, the larval behavior of seven Odonata species was studied in the laboratory to evaluate variations in frequency associated with sex and three types of water: drinking water, dechlorinated tap water, and water from the collection habitat. Larvae were maintained into containers at ambient temperature, 12h light, and food ad libitum. Larval behavior included motionless, swimming, feeding, body movements, walking, grooming, changes in the body orientation, perching, molting, suspending, and sitting and waiting to capture prey. Larvae showed the highest number of behaviors in higher turbidity water (Habitat Water treatment). Males and females showed similar frequency in behaviors. The more active were of the Sympetrum gilvumand Rhionaeschna cornigera species. Species showed higher frequencies in behaviors such as resting, eating, prey capture (Anisoptera), perching and walking (Zygoptera). The frequency of larval behaviors in higher turbidity is modulated by the capability to obtain food and simultaneously, avoid predators. Larvae show higher diversity of behaviors in increased turbidity because darker habitats could be more secure. Our study calls attention to the importance of addressing the effect of abiotic conditions on behaviors of aquatic insects, and how it can influence their ecological fitness. Finally, although we have achieved crucial advancements on molecular tools and sophisticated statistical routines, the basic information about behaviors facets it is still a need in ecological studies.
Alfuzosin vs. Silodosin in the Improvement of Ureteral Stent-Related Symptoms after Endoscopic Surgical Treatment of Urolithiasis: A Retrospective Comparison
Authors: Sirin Mehmet Emin , Yilmaz Mehmet , Polat Muhammed Emin , Olcucuoglu Erkan , Karaaslan Mustafa
Published: May 2023

Purpose In this study, we aimed to compare the efficacy of two α-blockers, alfuzosin and silodosin, which have different pharmacological properties despite being uroselective, in the treatment of double-J stent (DJS)-related symptoms.Material and methods Fifty male patients who underwent DJS implantation after ureterorenoscopy/retrograde intrarenal surgery were included in the study. The patients’ demographic and characteristic data were analyzed. All patients were asked to complete the validated Turkish version of the Ureteral Stent Symptom Questionnaire (USSQ), international prostate symptom score (IPSS), and international index of erectile function-5 (IIEF-5) the day before stent removal and again one month after stent removal. Statistical analysis of these variables was performed in the alfuzosin and silodosin groups, and a value of p < 0.05 was considered statistically significant.Results Whereas no side effects were observed in the alfuzosin group (Group A), retrograde ejaculation was observed in 8 (25.2%) patients in the silodosin group (Group S) (p = 0.006). Significant decreases in IPSS, IPSS-quality of life, and USSQ scores were observed in both Group A and Group S one month after stent removal (i.e., without stent) compared with before stent removal (i.e., with stent) (p < 0.05). Furthermore, a statistically significant increase in IIEF-5 score was observed in both groups after stent removal compared with IIEF-5 score with stenting (p < 0.05).Conclusion The DJS has a significant impact on patients’ quality of life and should only be used when indicated, not routinely after every endoscopic stone procedure. Considering that silodosin may cause more ejaculation problems than alfuzosin, we believe that both drugs can be used in male patients to resolve DJS-related symptoms.
The DNA damage response to radiological imaging: from ROS and γH2AX foci induction to gene expression responses in vivo
Authors: López-Riego Milagrosa , Płódowska Magdalena , Lis-Zajęcka Milena , Jeziorska Kamila , Tetela Sylwia , Węgierek-Ciuk Aneta , Sobota Daniel , Braziewicz Janusz , Lundholm Lovisa , Lisowska Halina , Wojcik Andrzej
Published: May 2023

Candidate ionising radiation exposure biomarkers must be validated in humans exposed in vivo. Blood from patients undergoing positron emission tomography - computed tomography scan (PET-CT) and skeletal scintigraphy (scintigraphy) was drawn before (0 h) and after (2 h) the procedure for correlation analyses of response of selected biomarkers with radiation dose and other available patient information. FDXR, CDKN1A, BBC3, GADD45A, XPCand MDM2 expression was determined by qRT-PCR, DNA damage (γH2AX) by flow cytometry, and reactive oxygen species (ROS) levels by flow cytometry using the 2', 7' – Dichlorofluorescin diacetate test in peripheral blood mononuclear cells (PBMC). For ROS experiments, 0- and 2 h samples were additionally exposed to UVA to determine whether diagnostic irradiation conditioned the response to further oxidative insult.
With some exceptions, radiological imaging induced weak γH2AX foci, ROS and gene expression fold changes, the latter with good coherence across genes within a patient. Diagnostic imaging did not influence oxidative stress in PBMC successively exposed to UVA. Correlation analyses with patient characteristics led to low correlation coefficient values. γH2AX fold change, which correlated positively with gene expression, presented a weak positive correlation with injected activity, indicating a radiation-induced subtle increase in DNA damage and subsequent activation of the DNA damage response pathway. The exposure discrimination potential of these biomarkers in the absence of control samples, as frequently demanded in radiological emergencies, was assessed using raw data. These results suggest that the variability of the response in heterogeneous populations might complicate identifying individuals exposed to low radiation doses.
Regulation of CagA-Helicobacter on Gastric PIM2 Expression in Gastric Cancer
Authors: Wang Haiyan , Cao Wenting , Lijuan Hong , Zhang Huijuan , Ni Peng , Chen Shuaiyin , Duan Guangcai , Liang Wenjuan , Zhang Rongguang
Published: May 2023

Infection with cagA-positiveH. pylori has been linked to an increased risk of gastric cancer. However, the precise mechanism by whichcagAregulates PIM2 expression in gastric cancer is currently unknown. To address this issue, a mutant NCTC11637ΔcagA strain ofH. pyloriwas constructed, and the effects of H. pylori/cagA on PIM2 expression in gastric cancer cells (HGC27, SGC7901, and AG) were examined. The results showed that co-culturing gastric cancer cells with NCTC11637 significantly increased PIM2 expression levels (P < 0.001) compared to the control group. Additionally, the expression of PIM2 in cells co-cultured with NCTC11637 was higher than that co-cultured with NCTC11637ΔcagA (P < 0.001). Furthermore, the eukaryotic expression vector pcDNA-cagA was successfully constructed, and its transfection into gastric cancer cells resulted in a significant increase in PIM2 mRNA expression levels compared to the control group after 48 hours. These findings suggest that H.pylori/CagA may play a crucial role in regulating PIM2 expression and therefore may contribute to the pathogenesis ofH. pyloriinfection.
CTSS is associated with immunity and affects prognosis in pan-cancer
Authors: Zhang De-yun , Liu Yu , Chen Zhongjun , Zheng Jinyu , Zhao Zongren
Published: May 2023

Background: CTSS(Cathepsisn S) is a lysosomal cysteine protease which is found in many tissues and plays multifarious roles. However, the role of CTSS in tumorigenesis is poorly defined. We investigated the association between CTSS levels and prognosis of cancer patients to determine prognostic value in this study.
Methods: In this study, the RNA-sequencing (RNA-Seq) gene expression profile and clinical data were downloaded from the TCGA database and the UCSC database. Immunohistochemical images of CTSS and its expression in normal tissues were obtained from HPA database.Statistical analysis and data visualization were performed on the relationship between CTSS expression and prognosis, TMB,MSI, immune score, immune checkpoint and enrichment pathway through R language version 4.1.1 and its additional package.
Results:Overall, CTSS is differentially expressed in most cancers and adjacent normal tissues.In addition, multiple survival analyses showed that CTSS significantly affected patient prognoses.CTSS was strongly associated with TMB in 8 cancers and MSI in 12 cancers. In addition, CTSS expression level is positively correlated with immune score in many cancers. Finally, GSEA analysis showed that CTSS was closely related to cell cycle, immune function, JAK-STAT and other biological functions and signaling pathways
Conclusion:In some tumor patients, the high expression of CTSS can be used as a biomarker with better prognosis.In contrast, high CTSS expression indicated poor prognosis in some tumors, especially in LGG. The relationship between CTSS and immune checkpoint and GSEA analysis of CTSS may provide some ideas for potential immunotherapy and related pathways of some tumors.
Endoscopic-assisted Laparoscopic Anterior Resection of Intussuscepting Primary Colonic Liposarcoma Shortened version: Endolap-assisted Anterior Resection of a Liposarcoma
Authors: Yeo Delphina Boon Xue , Lie Darius Aw Kang , Koh Frederick Xiang Hong , Foo Fung Joon , Ng Jia Lin
Published: May 2023

Background: Liposarcomas are uncommon malignant mesenchymal tumours arising in the extremities and sometimes in the retroperitoneum.
Methods: We discuss a case of a rare sigmoid colon liposarcoma in a lady who presented with constipation and tenesmus.
Results: In the case of a large liposarcoma, it is difficult to maneuver the mass without the risk of serosal tears. As we make an attempt in minimally-invasive surgery for this patient, we decided that it would be useful to utilize endoscopy to help reduce the mass and attempt to resect the mass laparoscopically without extensive serosal tears.
Conclusion: A hybrid endoscopic-laparoscopic high anterior resection can help to manipulate the large tumour and avoid a low anastomosis, thereby reducing the risk of an anastomotic leak when compared to a higher anastomosis.
Isolation And Molecular Characterization of Infectious Laryngotrachitis virus from Poultry in and Bishoftu town and the bordering District Liban Cuqala
Authors: Marga Galana Biratu
Published: May 2023

Infectious Laryngotrachitis is an important respiratory disease of chicken caused by gallid herpes virus-І belonged to family Herpesviridae, subfamily Alphaherpesvirinae, genus Iltovirus The disease has little or no previous documented data in the country. The study was conducted from November 2022 to June 2022 by a cross-sectional study design with purposive sampling strategy. In this study, a molecular detection of ILTV were conducted generally in 12 pooled samples out of the total 40 poultries sampled from peasant Associations (PAs) of in and around Bishoftu town and the bordering district Liban Cuqala. Swab samples from upper trachea and tracheal tissue samples were collected from the selected PAs in the study area. From the total 12 pooled samples, 3 samples were positively detected for the presence of Infectious Laryngotrachitis.The study revealed an overall PCR detection of 25%. Three strains of ILT namely ICP4, TCO low and TCO high were detected with the general master mix that can bind with all of the ILT strains. Generally, ILTV were one of the serious avian respiratory pathogen challenging the study areas resulted in high economic losses. Control and prevention measures through vaccination programmed should schedule within the viral strains.