bluuebunny/crossref_metadata_2025 huggingface dataset:
A Review of Green Building Development in China from the Perspective of Energy Saving
Authors: Zhang Ying , Kang Jian , Jin Hong
Published: February 2018

This paper reviews the history of green building development and assessment standards in China, particularly from the perspective of energy saving. It is divided into four parts: (1) the development of policies of green building in China that have been proposed for meeting energy-conservation and emission-reduction targets; (2) the scientific research on green building by the Chinese government, including the promotion of maximum resource sustainability, environmental protection, and the reduction of pollution; (3) the development of assessment standards for green building in China; and (4) the development of green building technologies in China.
Device Performance Improvement of Recycling Double-Pass Cross-Corrugated Solar Air Collectors
Authors: Ho Chii-Dong , Chang Hsuan , Hsiao Ching-Fang , Huang Chien-Chang
Published: February 2018

The device performance of cross-corrugated double-pass solar air heaters under external recycle conditions was investigated experimentally and theoretically, and solved numerically using the Newton method. Comparisons were made and represented graphically among three different configurations of the single-pass, flat-plate double-pass and cross-corrugated double-pass devices. Air flowing simultaneously over the wavelike corrugated absorbing plate and in-between both wavelike cross-corrugated absorbing and transverse bottom plates was conducted under double-pass operations. A considerable heat-transfer efficiency enhancement is obtained employing such a recycling double pass device with welding cross-corrugated absorbing plates, instead of using the flat-plate device. An effective thermal performance was achieved because the heat transfer area is doubled and the turbulent intensity is enhanced as well. The power consumption increment owing to the reduction in the cross-sectional area was taken into account associated with the heat-transfer efficiency enhancement for comparisons in determining the optimal design on an economic consideration for the recycling cross-corrugated double-pass device.
Influence of Reservoir Stimulation on Marine Gas Hydrate Conversion Efficiency in Different Accumulation Conditions
Authors: Yang Lin , Chen Chen , Jia Rui , Sun Youhong , Guo Wei , Pan Dongbin , Li Xitong , Chen Yong
Published: February 2018

In this paper, we used a method of combining reservoir stimulation technique (RST) with depressurization to investigate the conversion efficiency of marine natural gas hydrate (NGH) reservoirs in the Shenhu area, on the northern slope of the South China Sea, which differ in intrinsic permeability and initial NGH saturation conditions. We also analyzed the influence of the variable-stimulation effect on marine NGH conversion efficiency in different accumulation conditions, providing a reference scheme for improving the NGH conversion efficiency in the Shenhu area. In this work, we performed calculations for the variations in CH4 production rate and cumulative volume of CH4 in different initial NGH saturation, intrinsic permeability, and stimulation effect conditions. Variance analysis and range analysis methods were used to analyze the significance of these key factors and their interaction. Furthermore, we investigated the sensitivity of stimulation effect on NGH conversion efficiency. The simulation results showed that the stimulation effect has a significant influence on NGH conversion efficiency, and the influence of interaction between these three factors was not obvious. Possibly most importantly, we clarified that the sparsely fractured networks (N = 3) had a better effect for higher NGH conversion efficiency under higher saturation conditions. For lower permeability cases, the influence between sparsely (N = 3) and densely (N = 5) fractured networks were similar.
Continuous Steering Stability Control Based on an Energy-Saving Torque Distribution Algorithm for a Four in-Wheel-Motor Independent-Drive Electric Vehicle
Authors: Zhai Li , Hou Rufei , Sun Tianmin , Kavuma Steven
Published: February 2018

In this paper, a continuous steering stability controller based on an energy-saving torque distribution algorithm is proposed for a four in-wheel-motor independent-drive electric vehicle (4MIDEV) to improve the energy consumption efficiency while maintaining the stability in steering maneuvers. The controller is designed as a hierarchical structure, including the reference model level, the upper-level controller, and the lower-level controller. The upper-level controller adopts the direct yaw moment control (DYC), which is designed to work continuously during the steering maneuver to better ensure steering stability in extreme situations, rather than working only after the vehicle is judged to be unstable. An adaptive two-hierarchy energy-saving torque distribution algorithm is developed in the lower-level controller with the friction ellipse constraint as a basis for judging whether the algorithm needs to be switched, so as to achieve a more stable and energy-efficient steering operation. The proposed stability controller was validated in a co-simulation of CarSim and Matlab/Simulink. The simulation results under different steering maneuvers indicate that the proposed controller, compared with the conventional servo controller and the ordinary continuous controller, can reduce energy consumption up to 23.68% and improve the vehicle steering stability.
Effect of Nitrogen/Oxygen Substances on the Pyrolysis of Alkane-Rich Gases to Acetylene by Thermal Plasma
Authors: Huang Wei , Jin Junkui , Wen Guangdong , Yang Qiwei , Su Baogen , Ren Qilong
Published: February 2018

It is important to convert alkane-rich gases, such as coke oven gas, to value-added chemicals rather than direct emission or combustion. Abundant nitrogen/oxygen substances are present in the actual alkane-rich gases. However, the research about how they influence the conversion in the pyrolysis process is missing. In this work, a systematic investigation on the effect of various nitrogen/oxygen-containing substances, including N2, CO, and CO2,on the pyrolysis of CH4 to C2H2 was performed by a self-made 50 kW rotating arc thermal plasma reactor, and the pyrolysis of a simulated coke oven gas as a model of alkane-rich mixing gas was conducted as well. It was found that the presence of N2 and CO2 was not conducive to the main reaction of alkane pyrolysis for C2H2, while CO, as a stable equilibrium product, had little effect on the cracking reaction. Consequently, it is suggested that a pretreatment process of removing N2 and CO2 should be present before pyrolysis. Both input power and feed rate had considerable effect on the pyrolysis of the simulated coke oven gas, and a C2H2 selectivity of 91.2% and a yield of 68.3% could be obtained at an input power of 17.9 kW.
Preferences for Urban Green Space Characteristics: A Comparative Study in Three Portuguese Cities
Authors: Madureira Helena , Nunes Fernando , Oliveira José , Madureira Teresa
Published: February 2018

Many studies have argued that a better understanding of people’s preferences about public urban green spaces may inform urban planners to effectively provide and manage urban green spaces to meet users’ needs. The aim of this study is to examine urban residents preferred public green space characteristics and investigate whether similarities and differences can be highlighted in three different Portuguese cities. Through a web-based questionnaire based on the best-worst scaling (BWS) method, residents of Lisbon, Porto and Évora (n = 750) were asked to select the most and least important public green space characteristic among thirteen attributes. The results suggest a consensus about some green space characteristics across cities but also the existence of some local variations in city residents’ preferences. Overall, this study can support public authorities and urban planners as they strive to effectively design and manage urban green spaces to meet users’ needs.
Analysis of Global LAI/FPAR Products from VIIRS and MODIS Sensors for Spatio-Temporal Consistency and Uncertainty from 2012–2016
Authors: Xu Baodong , Park Taejin , Yan Kai , Chen Chi , Zeng Yelu , Song Wanjuan , Yin Gaofei , Li Jing , Liu Qinhuo , Knyazikhin Yuri , Myneni Ranga
Published: February 2018

The operational Moderate Resolution Imaging Spectroradiometer (MODIS) Leaf Area Index (LAI) and Fraction of Photosynthetically Active Radiation absorbed by vegetation (FPAR) algorithm has been successfully implemented for Visible Infrared Imager Radiometer Suite (VIIRS) observations by optimizing a small set of configurable parameters in Look-Up-Tables (LUTs). Our preliminary evaluation showed reasonable agreement between VIIRS and MODIS LAI/FPAR retrievals. However, there is a need for a more comprehensive investigation to assure continuity of multi-sensor global LAI/FPAR time series, as the preliminary evaluation was spatiotemporally limited. In this study, we use a multi-year (2012–2016) global LAI/FPAR product generated from VIIRS and MODIS to evaluate for spatiotemporal consistency. We also quantify uncertainty of the product by utilizing available ground measurements. For both consistency and uncertainty evaluation, we account for variations in biome type and temporal resolution. Our results indicate that the LAI/FPAR retrievals from VIIRS and MODIS are consistent at different spatial (i.e., global and site) and temporal (i.e., 8-day, seasonal and annual) scales. The estimate of mean discrepancy (−0.006 ± 0.013 for LAI and −0.002 ± 0.002 for FPAR) meets the stability requirement for long-term LAI/FPAR Earth System Data Records (ESDRs) from multi-sensors as suggested by the Global Climate Observing System (GCOS). It is noteworthy that the rate of retrievals from the radiative transfer-based main algorithm is also comparable between two sensors. However, a relatively larger discrepancy over tropical forests was observed due to reflectance saturation and an unexpected interannual variation of main algorithm success was noticed due to instability in input surface reflectances. The uncertainties/relative uncertainties of VIIRS and MODIS LAI (FPAR) products assessed through comparisons to ground measurements are estimated to be 0.60/42.2% (0.10/24.4%) and 0.55/39.3% (0.11/26%), respectively. Note that the validated LAI were only distributed in low domains (~2.5), resulting in large relative uncertainty. Therefore, more ground measurements are needed to achieve a more comprehensive evaluation result of product uncertainty. The results presented here generally imbue confidence in the consistency between VIIRS and MODIS LAI/FPAR products and the feasibility of generating long-term multi-sensor LAI/FPAR ESDRs time series.
Night Light-Adaptation Strategies for Photosynthetic Apparatus in Yellow-Poplar (Liriodendron tulipifera L.) Exposed to Artificial Night Lighting
Authors: Kwak Myeong , Je Sun , Cheng Hyo , Seo Se , Park Jeong , Baek Saeng , Khaine Inkyin , Lee Taeyoon , Jang Jihwi , Li Yang , Kim Haenaem , Lee Jong , Kim Jieun , Woo Su
Published: February 2018

Plants can undergo external fluctuations in the natural light and dark cycle. The photosynthetic apparatus needs to operate in an appropriate manner to fluctuating environmental factors, especially in light. Yellow-poplar seedlings were exposed to nighttime artificial high-pressure sodium (HPS) lighting to evaluate night light-adaptation strategies for photosynthetic apparatus fitness relative to pigment contents, photosystem II photochemistry, photosynthetic parameters, histochemical analysis of reactive oxygen species, and plant biomass. As a result, seedlings exhibited dynamic changes including the enhancement of accessory pigments, the reduction of photosystem II photochemistry, increased stomatal limitation, downregulation of photosynthesis, and the decreased aboveground and belowground biomass under artificial night lighting. Histochemical analysis with 3,3′-diaminobenzidine (DAB) and nitroblue tetrazolium (NBT) staining indicates the accumulation of in situ superoxide radicals (O2−) and hydrogen peroxide (H2O2) in leaves exposed to the lowest level of artificial night lighting compared to control. Moreover, these leaves exposed to artificial night lighting had a lower nighttime respiration rate. These results indicated that HPS lighting during the night may act as a major factor as repressors of the fitness of photosynthesis and growth patterns, via a modification of the photosynthetic light harvesting apparatus.
Strategies to Extend Bread and GF Bread Shelf-Life: From Sourdough to Antimicrobial Active Packaging and Nanotechnology
Authors: Melini Valentina , Melini Francesca
Published: February 2018

Bread is a staple food worldwide. It commonly undergoes physico-chemical and microbiological changes which impair its quality and shelf-life. Staling determines organoleptic impairment, whereas microbiological spoilage causes visible mould growth and invisible production of mycotoxins. To tackle this economic and safety issue, the bakery industry has been working to identify treatments which allow bread safety and extended shelf-life. Physical methods and chemical preservatives have long been used. However, new frontiers have been recently explored. Sourdough turned out an ancient but novel technology to preserve standard and gluten-free bread. Promising results have also been obtained by application of alternative bio-preservation techniques, including antifungal peptides and plant extracts. Active packaging, with absorbing and/or releasing compounds effective against bread staling and/or with antimicrobials preventing growth of undesirable microorganisms, showed up an emerging area of food technology which can confer many preservation benefits. Nanotechnologies are also opening up a whole universe of new possibilities for the food industry and the consumers. This work thus aims to provide an overview of opportunities and challenges that traditional and innovative anti-staling and anti-spoilage methods can offer to extend bread shelf-life and to provide a basis for driving further research on nanotechnology applications into the bakery industry.
Transcriptome Assembly of the Bast Fiber Crop, Ramie, Boehmeria nivea (L.) Gaud. (Urticaceae)
Authors: Al-Ani Louay , Deyholos Michael
Published: February 2018

Ramie (Boehmeria nivea) is a perennial crop valued for its strong bast fibers. Unlike other major bast fiber crops, ramie fiber processing does not include retting, but does require degumming, suggesting distinctive features in pectin and the development and composition of fibers. A comprehensive transcriptome assembly of ramie has not been made available, to date. We obtained the sequence of RNA transcripts (RNA Seq) from the apical region of developing ramie stems and combined these with reads from public databases for a total of 157,621,051 paired-end reads (30.3 billion base pairs Gbp) used as input for de novo assembly, resulting in 70,721 scaffolds (≥200 base pairs (bp); N50 = 1798 bp). As evidence of the quality of the assembly, 36,535 scaffolds aligned to at least one Arabidopsis protein (BLASTP e-value ≤ 10−10). The resource described here for B. nivea will facilitate an improved understanding of bast fibers, cell wall, and middle lamella development in this and other comparative species.
Effect of Polypropylene Fibers on Self-Healing and Dynamic Modulus of Elasticity Recovery of Fiber Reinforced Concrete
Authors: El-Newihy Adham , Azarsa Pejman , Gupta Rishi , Biparva Alireza
Published: February 2018

This study aims to evaluate self-healing properties and recovered dynamic moduli of engineered polypropylene fiber reinforced concrete using non-destructive resonant frequency testing. Two types of polypropylene fibers (0.3% micro and 0.6% macro) and two curing conditions have been investigated: Water curing (at ~25 Celsius) and air curing. The Impact Resonance Method (IRM) has been conducted in both transverse and longitudinal modes on concrete cylinders prior/post crack induction and post healing of cracks. Specimens were pre-cracked at 14 days, obtaining values of crack width in the range of 0.10–0.50 mm. Addition of polypropylene fibers improved the dynamic response of concrete post-cracking by maintaining a fraction of the original resonant frequency and elastic properties. Macro fibers showed better improvement in crack bridging while micro fiber showed a significant recovery of the elastic properties. The results also indicated that air-cured Polypropylene Fiber Reinforced Concrete (PFRC) cylinders produced ~300 Hz lower resonant frequencies when compared to water-cured cylinders. The analyses showed that those specimens with micro fibers exhibited a higher recovery of dynamic elastic moduli.
Advancing Fire Science with Large Forest Plots and a Long-Term Multidisciplinary Approach
Authors: Lutz James , Larson Andrew , Swanson Mark
Published: February 2018

Large, spatially explicit forest plots have the potential to address currently understudied aspects of fire ecology and management, including the validation of physics-based fire behavior models and next-generation fire effects models. Pre-fire forest structures, fire-mediated mortality, and post-fire forest development can be examined in a spatial context, and value can be added to current multidisciplinary approaches by adding a long-term perspective. Here we propose that the fire science community begin to build a collaborative network of fire-related large forest dynamics plots to examine explicit spatial patterns of surface fuels, tree mortality, and post-fire regeneration throughout ecosystems with frequent-fire forests.
Fluid Dynamics and Mass Transfer in Spacer-Filled Membrane Channels: Effect of Uniform Channel-Gap Reduction Due to Fouling
Authors: Koutsou Chrysafenia , Karabelas Anastasios , Kostoglou Margaritis
Published: February 2018

The time-varying flow field in spacer-filled channels of spiral-wound membrane (SWM) modules is mainly due to the development of fouling layers on the membranes that modify the channel geometry. The present study is part of an approach to tackling this extremely difficult dynamic problem at a small spatial scale, by uncoupling the fluid dynamics and mass transfer from the fouling-layer growth process. Therefore, fluid dynamics and mass transfer are studied for a spacer-filled channel whose geometry is altered by a uniform deposit thickness h. For this purpose, 3D direct numerical simulations are performed employing the “unit cell” approach with periodic boundary conditions. Specific thickness values are considered in the range 2.5–10% of the spacer-filament diameter D as well as other conditions of practical significance. The qualitative characteristics of the altered flow field are found to be very similar to those of the reference geometry with no gap reduction. For a given flow rate, the pressure drop, time-average wall-shear stresses and mass-transfer coefficients significantly increase with increasing thickness h due to reduced channel-gap, as expected. Correlations are obtained, applicable at the “unit cell” scale, of the friction factor f and Sherwood number Sh, which exhibit similar functional dependence of f and Sh on the Reynolds and Schmidt numbers as in the reference no-fouling case. In these correlations the effect of channel-gap reduction is incorporated, permitting predictions in the studied range of fouling-layer thickness (h/D) = 0–0.10. The usefulness of the new results and correlations is discussed in the context of ongoing research toward improved modeling and dynamic simulation of SWM-module operation.
Fermentability of Novel Type-4 Resistant Starches in In Vitro System
Authors: Erickson Jennifer , Carlson Justin , Stewart Maria , Slavin Joanne
Published: February 2018

Resistant starches are non-digestible starches that are fermented in the colon by microbiota. These carbohydrates are prebiotic and can be beneficial to consumer health. Many types of resistant starch exist with varying physical properties that may result in differences in fermentability. The objective of this research project was to compare potential prebiotic effects and fermentability of four novel resistant starches using an in vitro fermentation system and measuring changes in total gas production, pH, and formation of SCFAs (short chain fatty acids). Fecal donations were collected from seven healthy volunteers. Four novel resistant starches, modified potato starch (MPS), modified tapioca starch (MTS), and modified maize starches (MMS-1 and MMS-2), were analyzed and compared to polydextrose and short chain fructooligosaccharides (FOS) as controls. After twenty-four hours of fermentation, MPS and MTS responded similarly in gas production (74 mL; 70.6 mL respectively), pH (5.93; 5.93 respectively), and SCFA production (Acetate: 115; 124, Propionate: 21; 26, Butyrate: 29; 31 μmol/mL respectively). While MMS-1 had similar gas production and individual SCFA production, the pH was significantly higher (6.06). The fermentation of MMS-2 produced the least amount of gas (22 mL), with a higher pH (6.34), and lower acetate production (78.4 μmol/mL). All analyzed compounds were fermentable and promoted the formation of beneficial SCFAs.
Stimuli-Responsive Cationic Hydrogels in Drug Delivery Applications
Authors: Deen G. , Loh Xian
Published: February 2018

Stimuli-responsive, smart, intelligent, or environmentally sensitive polymers respond to changes in external stimuli such as pH, temperature, ionic strength, surfactants, pressure, light, biomolecules, and magnetic field. These materials are developed in various network architectures such as block copolymers, crosslinked hydrogels, nanogels, inter-penetrating networks, and dendrimers. Stimuli-responsive cationic polymers and hydrogels are an interesting class of “smart” materials that respond reversibly to changes in external pH. These materials have the ability to swell extensively in solutions of acidic pH and de-swell or shrink in solutions of alkaline pH. This reversible swelling-shrinking property brought about by changes in external pH conditions makes these materials useful in a wide range of applications such as drug delivery systems and chemical sensors. This article focuses mainly on the properties of these interesting materials and their applications in drug delivery systems.
Non-Conventional Methods for Gelation of Alginate
Authors: Gurikov Pavel , Smirnova Irina
Published: February 2018

This review presents and critically evaluates recent advances in non-conventional gelation method of native alginate. A special focus is given to the following three methods: cryotropic gelation, non-solvent induced phase separation and carbon dioxide induced gelation. A few other gelation approaches are also briefly reviewed. Results are discussed in the context of subsequent freeze and supercritical drying. The methods are selected so as to provide the readers with a range of novel tools and tactics of pore engineering for alginate and other anionic polysaccharides.
The Role of Motivation to Reduce Obesity among Elderly People: Response to Priming Temptation in Obese Individuals
Authors: Obara-Gołębiowska Małgorzata , Brycz Hanna , Lipowska Małgorzata , Lipowski Mariusz
Published: February 2018

The risk of obesity-related disorders is increased among the elderly, so changing eating habits can be an important element of prevention. The main aim of this article is to consider whether looking at pictures that present either fattening food or healthy food may motivate elderly people to change their nutrition habits. Might priming different kinds of food influence the attractiveness of the food for people in late adulthood undergoing obesity therapy? Based on priming theories, we analysed the effects of the conscious processing of stimuli associated with dietary habits in individuals aged with BMI ≥ 30 kg/m2. Our experiments confirmed the influence of a higher-priority goal of “slimming” on the perception and internalization of nutrition-related stimuli. In response to such stimuli, individuals who are actively involved in weight reduction and health-oriented programs use strategies for resisting temptation and to effectively “slim”. We present our findings in the context of their theoretical background and practical application.
Spatio-Temporal Dynamic Analysis of Sustainable Development in China Based on the Footprint Family
Authors: Zhao Jing , Ma Caihong , Zhao Xiangui , Wang Xiaoyu
Published: February 2018

The existing index systems on sustainable evaluation are mostly based on a multi index comprehensive evaluation method. The main disadvantage of this approach is that the selection and assignment of evaluation indexes are greatly influenced by subjective factors, which can result in poor comparability of results. By contrast, the Footprint Family (including ecological footprint, carbon footprint, and water footprint) is not affected by subjective factors. The Footprint Family also covers the basic tenets of sustainable development. This paper proposes use of a sustainable development evaluation index system based on the principle of the Footprint Family, and including the ecological pressure index (EPI), the ecological occupancy index (EOI), the ecological economic coordination index (EECI), the GHG (Greenhouse Gas) emission index (CEI), the water resources stress index (WSI), and the sustainable development index (SDI). Furthermore, a standard for grading the evaluated results based on global benchmarks is formulated. The results of an empirical study in China were the following. The development situation deteriorated from 1990 to 2015. The results showed that the SDI decreased from a medium level (grade 5) to a lower-medium level (grade 4). The results of this empirical study also showed that the method of evaluation can avoid the influence of subjective factors and can be used in the evaluation of sustainable development for various temporal and spatial conditions.
Bank Ownership, Board Characteristics and Performance: Evidence from Commercial Banks in India
Authors: Sarkar Jayati , Sarkar Subrata
Published: February 2018

We study the effect of board governance in state-owned and private banks by undertaking a study of commercial banks in India that has both bank groups. Covering a ten-year period from 2003 to 2012 that witnessed a large number of governance reforms in India, the results of our empirical analysis provide evidence of strong ownership effects with board independence exhibiting a significant positive correlation with the performance of private banks and a significant but negative correlation with the performance of state-owned banks. The effect of CEO duality is negative in state-owned banks where incidence of CEO duality is high. We find that a longer CEO tenure has significant positive effects on bank outcomes with these effects strengthening in the later years of CEO tenure. Our results have governance implications for strengthening the composition of board of directors and CEO tenure especially in state-owned banks.
A Phenotyping Method of Giant Cells from Root-Knot Nematode Feeding Sites by Confocal Microscopy Highlights a Role for CHITINASE-LIKE 1 in Arabidopsis
Authors: Cabrera Javier , Olmo Rocio , Ruiz-Ferrer Virginia , Abreu Isidro , Hermans Christian , Martinez-Argudo Isabel , Fenoll Carmen , Escobar Carolina
Published: February 2018

Most effective nematicides for the control of root-knot nematodes are banned, which demands a better understanding of the plant-nematode interaction. Understanding how gene expression in the nematode-feeding sites relates to morphological features may assist a better characterization of the interaction. However, nematode-induced galls resulting from cell-proliferation and hypertrophy hinders such observation, which would require tissue sectioning or clearing. We demonstrate that a method based on the green auto-fluorescence produced by glutaraldehyde and the tissue-clearing properties of benzyl-alcohol/benzyl-benzoate preserves the structure of the nematode-feeding sites and the plant-nematode interface with unprecedented resolution quality. This allowed us to obtain detailed measurements of the giant cells’ area in an Arabidopsis line overexpressing CHITINASE-LIKE-1 (CTL1) from optical sections by confocal microscopy, assigning a role for CTL1 and adding essential data to the scarce information of the role of gene repression in giant cells. Furthermore, subcellular structures and features of the nematodes body and tissues from thick organs formed after different biotic interactions, i.e., galls, syncytia, and nodules, were clearly distinguished without embedding or sectioning in different plant species (Arabidopsis, cucumber or Medicago). The combination of this method with molecular studies will be valuable for a better understanding of the plant-biotic interactions.
Analysis of Transcriptional Responses of the Inflorescence Meristems in Jatropha curcas Following Gibberellin Treatment
Authors: Hui Wen-Kai , Wang Yi , Chen Xiao-Yang , Zayed Mohamed , Wu Guo-Jiang
Published: February 2018

Jatropha curcas L. seeds an oilseed plant with great potential for biodiesel production. However, low seed yield, which was limited by its lower female flowers, was a major drawback for its utilization. Our previous study found that the flower number and female-to-male ratio were increased by gibberellin treatment. Here, we compared the transcriptomic profiles of inflorescence meristem at different time points after gibberellic acid A3 (GA3) treatment. The present study showed that 951 differentially expressed genes were obtained in response to gibberellin treatment, compared with control samples. The 6-h time point was an important phase in the response to exogenous gibberellin. Furthermore, the plant endogenous gibberellin, auxin, ethylene, abscisic acid, and brassinolide-signaling transduction pathways were repressed, whereas the genes associated with cytokinin and jasmonic acid signaling were upregulated for 24-h time point following GA3 treatment. In addition, the floral meristem determinacy genes (JcLFY, JcSOC1) and floral organ identity genes (JcAP3, JcPI, JcSEP1-3) were significantly upregulated, but their negative regulator (JcSVP) was downregulated after GA3 treatment. Moreover, the effects of phytohormone, which was induced by exogenous plant growth regulator, mainly acted on the female floral differentiation process. To the best of our knowledge, this data is the first comprehensive analysis of the underlying transcriptional response mechanism of floral differentiation following GA3 treatment in J. curcas, which helps in engineering high-yielding varieties of Jatropha.
When Immune Cells Turn Bad—Tumor-Associated Microglia/Macrophages in Glioma
Authors: Roesch Saskia , Rapp Carmen , Dettling Steffen , Herold-Mende Christel
Published: February 2018

As a substantial part of the brain tumor microenvironment (TME), glioma-associated microglia/macrophages (GAMs) have an emerging role in tumor progression and in controlling anti-tumor immune responses. We review challenges and improvements of cell models and highlight the contribution of this highly plastic cell population to an immunosuppressive TME, besides their well-known functional role regarding glioma cell invasion and angiogenesis. Finally, we summarize first therapeutic interventions to target GAMs and their effect on the immunobiology of gliomas, focusing on their interaction with T cells.
Indole-3-Acetic Acid Biosynthesis Pathways in the Plant-Beneficial Bacterium Arthrobacter pascens ZZ21
Authors: Li Mengsha , Guo Rui , Yu Fei , Chen Xu , Zhao Haiyan , Li Huixin , Wu Jun
Published: February 2018

Arthrobacter pascens ZZ21 is a plant-beneficial, fluoranthene-degrading bacterial strain found in the rhizosphere. The production of the phytohormone indole-3-aectic acid (IAA) by ZZ21 is thought to contribute to its ability to promote plant growth and remediate fluoranthene-contaminated soil. Using genome-wide analysis combined with metabolomic and high-performance liquid chromatography-mass spectrometry (HPLC-MS) analyses, we characterized the potential IAA biosynthesis pathways in A. pascens ZZ21. IAA production increased 4.5-fold in the presence of 200 mg·L−1 tryptophan in the culture medium. The transcript levels of prr and aldH, genes which were predicted to encode aldehyde dehydrogenases, were significantly upregulated in response to exogenous tryptophan. Additionally, metabolomic analysis identified the intermediates indole-3-acetamide (IAM), indole-3-pyruvic acid (IPyA), and the enzymatic reduction product of the latter, indole-3-lactic acid (ILA), among the metabolites of ZZ21, and subsequently also IAM, ILA, and indole-3-ethanol (TOL), which is the enzymatic reduction product of indole-3-acetaldehyde, by HPLC-MS. These results suggest that the tryptophan-dependent IAM and IPyA pathways function in ZZ21.
Galectin-3: The Impact on the Clinical Management of Patients with Thyroid Nodules and Future Perspectives
Authors: Bartolazzi Armando , Sciacchitano Salvatore , D’Alessandria Calogero
Published: February 2018

Galectins (S-type lectins) are an evolutionarily-conserved family of lectin molecules, which can be expressed intracellularly and in the extracellular matrix, as well. Galectins bind β-galactose-containing glycoconjugates and are functionally active in converting glycan-related information into cell biological programs. Altered glycosylation notably occurring in cancer cells and expression of specific galectins provide, indeed, a fashionable mechanism of molecular interactions able to regulate several tumor relevant functions, among which are cell adhesion and migration, cell differentiation, gene transcription and RNA splicing, cell cycle and apoptosis. Furthermore, several galectin molecules also play a role in regulating the immune response. These functions are strongly dependent on the cell context, in which specific galectins and related glyco-ligands are expressed. Thyroid cancer likely represents the paradigmatic tumor model in which experimental studies on galectins’ glycobiology, in particular on galectin-3 expression and function, contributed greatly to the improvement of cancer diagnosis. The discovery of a restricted expression of galectin-3 in well-differentiated thyroid carcinomas (WDTC), compared to normal and benign thyroid conditions, contributed also to promoting preclinical studies aimed at exploring new strategies for imaging thyroid cancer in vivo based on galectin-3 immuno-targeting. Results derived from these recent experimental studies promise a further improvement of both thyroid cancer diagnosis and therapy in the near future. In this review, the biological role of galectin-3 expression in thyroid cancer, the validation and translation to a clinical setting of a galectin-3 test method for the preoperative characterization of thyroid nodules and a galectin-3-based immuno-positron emission tomography (immuno-PET) imaging of thyroid cancer in vivo are presented and discussed.
Temporomandibular Joint Regenerative Medicine
Authors: Van Bellinghen Xavier , Idoux-Gillet Ysia , Pugliano Marion , Strub Marion , Bornert Fabien , Clauss Francois , Schwinté Pascale , Keller Laetitia , Benkirane-Jessel Nadia , Kuchler-Bopp Sabine , Lutz Jean , Fioretti Florence
Published: February 2018

The temporomandibular joint (TMJ) is an articulation formed between the temporal bone and the mandibular condyle which is commonly affected. These affections are often so painful during fundamental oral activities that patients have lower quality of life. Limitations of therapeutics for severe TMJ diseases have led to increased interest in regenerative strategies combining stem cells, implantable scaffolds and well-targeting bioactive molecules. To succeed in functional and structural regeneration of TMJ is very challenging. Innovative strategies and biomaterials are absolutely crucial because TMJ can be considered as one of the most difficult tissues to regenerate due to its limited healing capacity, its unique histological and structural properties and the necessity for long-term prevention of its ossified or fibrous adhesions. The ideal approach for TMJ regeneration is a unique scaffold functionalized with an osteochondral molecular gradient containing a single stem cell population able to undergo osteogenic and chondrogenic differentiation such as BMSCs, ADSCs or DPSCs. The key for this complex regeneration is the functionalization with active molecules such as IGF-1, TGF-β1 or bFGF. This regeneration can be optimized by nano/micro-assisted functionalization and by spatiotemporal drug delivery systems orchestrating the 3D formation of TMJ tissues.
Luteolin Inhibits Tumorigenesis and Induces Apoptosis of Non-Small Cell Lung Cancer Cells via Regulation of MicroRNA-34a-5p
Authors: Jiang Ze-Qun , Li Mu-Han , Qin Yue-Mu , Jiang Hai-Ying , Zhang Xu , Wu Mian-Hua
Published: February 2018

Luteolin (LTL) exerts remarkable tumor suppressive activity on various types of cancers, including non-small cell lung cancer (NSCLC). However, it is not completely understood whether the mechanism of its action against NSCLC is related to microRNAs (miRNAs). In the present study, we investigated the anti-tumor effects of LTL on NSCLC in vitro and in vivo. The results revealed that LTL could inhibit cell proliferation and induce apoptosis in both A549 and H460 cells. In a H460 xenograft tumor model of nude mice, LTL significantly suppressed tumor growth, inhibited cell proliferation, and induced apoptosis. miRNA microarray and quantitative PCR (qPCR) analysis indicated that miR-34a-5p was dramatically upregulated upon LTL treatment in tumor tissues. Furthermore, MDM4 was proved to be a direct target of miR-34a-5p by luciferase reporter gene assay. LTL treatment was associated with increased p53 and p21 protein expressions and decreased MDM4 protein expression in both NSCLC cells and tumor tissues. When miR-34a-5p was inhibited in vitro, the protein expressions of Bcl-2 and MDM4 were recovered, while that of p53, p21, and Bax were attenuated. Moreover, caspase-3 and caspase-9 activation induced by LHL treatment in vitro were also suppressed by miR-34a-5p inhibition. Overall, LTL could inhibit tumorigenesis and induce apoptosis of NSCLC cells by upregulation of miR-34a-5p via targeting MDM4. These findings provide novel insight into the molecular functions of LTL that suggest its potential as a therapeutic agent for human NSCLC.
Common Variable Immunodeficiency and Gastric Malignancies
Authors: Leone Patrizia , Vacca Angelo , Dammacco Franco , Racanelli Vito
Published: February 2018

Common variable immunodeficiency (CVID) is an immunodeficiency disorder with a high incidence of gastrointestinal manifestations and an increased risk of gastric carcinoma and lymphoma. This review discusses the latest advancements into the immunological, clinical and diagnostic aspects of gastric malignancies in patients with CVID. The exact molecular pathways underlying the relationships between CVID and gastric malignancies remain poorly understood. These include genetics, immune dysregulation and chronic infections by Helicobacter pylori. Further studies are needed to better stratify the risk for cancer in these patients, to elaborate surveillance programs aimed at preventing these complications, and to develop new and more effective therapeutic approaches.
Black Border Increases Stomoxys calcitrans Catch on White Sticky Traps
Authors: Murchie Archie , Hall Carol , Gordon Alan , Clawson Sam
Published: February 2018

Stable fly, Stomoxys calcitrans, is a biting fly that can cause severe irritation to livestock resulting in reduced productivity. The most common method of monitoring S. calcitrans is through the use of sticky traps and many designs have been developed using different colours and materials such as alsynite fibreglass and polypropylene sheeting. Laboratory experiments and some field experimentation have demonstrated that colour contrast can attract S. calcitrans. However, this response has not been fully utilised in trap design. To test that simple colour contrast could increase trap efficacy, white sticky traps were mounted on three differently coloured backgrounds (white, yellow, and black) and positioned at five sites on a mixed livestock farm. White sticky traps on a black background caught significantly more S. calcitrans than the yellow or white backgrounds. An incidental result was that Pollenia sp. were caught in greater numbers on the yellow framed traps. The reasons for S. calcitrans attraction to black–white contrast are most likely due to conspicuousness in the environment although the extent to which flies are using this feature as a host-location cue or a perching site are unknown.
Assessing Static and Dynamic Response Variability due to Parametric Uncertainty on Fibre-Reinforced Composites
Authors: Carvalho Alda , Silva Tiago , Loja Maria
Published: February 2018

Composite structures are known for their ability to be tailored according to specific operating requisites. Therefore, when modelling these types of structures or components, it is important to account for their response variability, which is mainly due to significant parametric uncertainty compared to traditional materials. The possibility of manufacturing a material according to certain needs provides greater flexibility in design but it also introduces additional sources of uncertainty. Regardless of the origin of the material and/or geometrical variabilities, they will influence the structural responses. Therefore, it is important to anticipate and quantify these uncertainties as much as possible. With the present work, we intend to assess the influence of uncertain material and geometrical parameters on the responses of composite structures. Behind this characterization, linear static and free vibration analyses are performed considering that several material properties, the thickness of each layer and the fibre orientation angles are deemed to be uncertain. In this study, multivariable linear regression models are used to model the maximum transverse deflection and fundamental frequency for a given set of plates, aiming at characterizing the contribution of each modelling parameter to the explanation of the response variability. A set of simulations and numerical results are presented and discussed.
Towards Additive Manufacture of Functional, Spline-Based Morphometric Models of Healthy and Diseased Coronary Arteries: In Vitro Proof-of-Concept Using a Porcine Template
Authors: Jewkes Rachel , Burton Hanna , Espino Daniel
Published: February 2018

The aim of this study is to assess the additive manufacture of morphometric models of healthy and diseased coronary arteries. Using a dissected porcine coronary artery, a model was developed with the use of computer aided engineering, with splines used to design arteries in health and disease. The model was altered to demonstrate four cases of stenosis displaying varying severity, based on published morphometric data available. Both an Objet Eden 250 printer and a Solidscape 3Z Pro printer were used in this analysis. A wax printed model was set into a flexible thermoplastic and was valuable for experimental testing with helical flow patterns observed in healthy models, dominating the distal LAD (left anterior descending) and left circumflex arteries. Recirculation zones were detected in all models, but were visibly larger in the stenosed cases. Resin models provide useful analytical tools for understanding the spatial relationships of blood vessels, and could be applied to preoperative planning techniques, but were not suitable for physical testing. In conclusion, it is feasible to develop blood vessel models enabling experimental work; further, through additive manufacture of bio-compatible materials, there is the possibility of manufacturing customized replacement arteries.
Partition and Inclusion Hierarchies of Images: A Comprehensive Survey
Authors: Bosilj Petra , Kijak Ewa , Lefèvre Sébastien
Published: February 2018

The theory of hierarchical image representations has been well-established in Mathematical Morphology, and provides a suitable framework to handle images through objects or regions taking into account their scale. Such approaches have increased in popularity and been favourably compared to treating individual image elements in various domains and applications. This survey paper presents the development of hierarchical image representations over the last 20 years using the framework of component trees. We introduce two classes of component trees, partitioning and inclusion trees, and describe their general characteristics and differences. Examples of hierarchies for each of the classes are compared, with the resulting study aiming to serve as a guideline when choosing a hierarchical image representation for any application and image domain.
How Well Do AR5 Sea Surface-Height Model Projections Match Observational Rates of Sea-Level Rise at the Regional Scale?
Authors: Watson Phil
Published: February 2018

The reliance upon and importance of climate models continues to grow in line with strengthening evidence of a changing climate system and the necessity to provide credible projections for risk assessment to guide policy development, mitigation and adaptation responses. The utility of the models to project regional rates of sea-level rise over the course of the 21st century is reliant on evaluating model outputs against global observational data (principally altimetry products). This study compares rates of sea-level rise from observational data records (tide gauges) against the ensemble mean of the model-projection products used in AR5 at 19 sites around the world over the decade of common data coverage (2007–2016) using enhanced time-series analysis techniques. Although it could be concluded that the observational and model-projected average velocity agree (95% confidence level (CL)), error margins are comparatively wide, masking the fact that the mean velocity for the model-projection products exceed observational records for nearly all stations and Representative Concentration Pathway (RCP) experiments, and are likely in the range of 1.6–2.5 mm/year. The analysis might provide an early warning sign that the evaluation of ocean model components with respect to projected mean sea level could be relevantly improved.
Current Knowledge and Recent Advances in Marine Dinoflagellate Transcriptomic Research
Authors: Akbar Muhamad , Ahmad Asmat , Usup Gires , Bunawan Hamidun
Published: February 2018

Dinoflagellates are essential components in marine ecosystems, and they possess two dissimilar flagella to facilitate movement. Dinoflagellates are major components of marine food webs and of extreme importance in balancing the ecosystem energy flux in oceans. They have been reported to be the primary cause of harmful algae bloom (HABs) events around the world, causing seafood poisoning and therefore having a direct impact on human health. Interestingly, dinoflagellates in the genus Symbiodinium are major components of coral reef foundations. Knowledge regarding their genes and genome organization is currently limited due to their large genome size and other genetic and cytological characteristics that hinder whole genome sequencing of dinoflagellates. Transcriptomic approaches and genetic analyses have been employed to unravel the physiological and metabolic characteristics of dinoflagellates and their complexity. In this review, we summarize the current knowledge and findings from transcriptomic studies to understand the cell growth, effects on environmental stress, toxin biosynthesis, dynamic of HABs, phylogeny and endosymbiosis of dinoflagellates. With the advancement of high throughput sequencing technologies and lower cost of sequencing, transcriptomic approaches will likely deepen our understanding in other aspects of dinoflagellates’ molecular biology such as gene functional analysis, systems biology and development of model organisms.