bluuebunny/crossref_metadata_2025 huggingface dataset:
Social Acceptance of Renewable Energy Technologies in the Post-fukushima Era
Authors: Park Eunil
Published: January 2021

In 2011, the Fukushima nuclear accident occurred, and this had a strong effect on public perceptions of energy facilities and services that relate not only to nuclear energy, but also renewable energy resources. Moreover, the accident has also considerably affected national energy plans in both developing and developed countries. In South Korea, several studies have been conducted since the accident to investigate public perspectives toward particular energy technologies; however, few studies have investigated public perceptions of renewable-energy technologies and tracked the transitions. Therefore, this study examines the trend of South Korean public’s perceptions of renewable-energy technologies. Based on data collected in 2016, we validated the structural connections and determined that trust, benefits, risks, and attitude were key determinants of the public’s desire to adopt these technologies; specifically, public attitude was found to be the greatest determinant of this desire. Based on the results, both implications and limitations are examined.
Working Memory Alterations Plays an Essential Role in Developing Global Neuropsychological Impairment in Duchenne Muscular Dystrophy
Authors: Tyagi Rahul , Arvind Harshita , Goyal Manoj , Anand Akshay , Mohanty Manju
Published: January 2021

Background

Neuropsychological profile of Indian Duchenne muscular dystrophy (DMD) subjects remains unidentified and needs to be evaluated.

Methods

A total of 69 DMD and 66 controls were subjected to detailed intelligence and neuropsychological assessment. The factor indexes were derived from various components of Malin’s Intelligence Scale for Indian Children (MISIC) and Rey Auditory Verbal Learning Test (RAVLT).

Results

Poor verbal and visual memory profiles were demonstrated by DMDs, which include RAVLT-immediate recall (IR) (p = 0.042), RAVLT-delayed recall (DR) (p = 0.009), Rey–Osterrieth complex figure test (RCFT)-IR (p = 0.001), and RCFT-DR (p = 0.001). RAVLT-memory efficiency index demonstrated poor verbal memory efficiency (p = 0.008). Significant differences in the functioning of working memory axis [RAVLT T1 (p = 0.015), recency T1 (p = 0.004), Digit Span Backward (p = 0.103)] were observed along with reduced performance in visuomotor coordination, visuospatial, and visual recognition abilities. Block designing efficiency index and attention fraction showed a normal performance in DMD kids.

Conclusion

Working memory deficits were found to be the crucial element of cognitive functioning in DMD cases. Working memory interventions may be beneficial to improve the neuropsychological profile in DMD.
Livestock Mobility Through Integrated Beef Production-Scapes Supports Rangeland Livestock Production and Conservation
Authors: Barry Sheila
Published: January 2021

Much of the world's rangelands contribute to food production through extensive grazing systems. In these systems, livestock producers, pastoralists, and ranchers move grazing animals to access variable feed and water resources to create value while supporting numerous other ecosystem services. Loss of mobility due to political, social, ecological, and economic factors is documented throughout the world and poses a substantial risk to rangeland livestock production and conservation of rangeland resources. The integration of production-scapes can facilitate livestock mobility through transportation and trade. This paper describes the beef cattle production system in California, where transporting and marketing animals integrate an extensive grazing system with intensive production systems, including feeding operations. Analysis of livestock inspection data quantifies the magnitude of livestock movements in the state and the scope of production-system integration. Over 500,000 head−47 percent of the state's calf crop—leave California rangelands and are moved to new pastures or feedyards seasonally over a 12 week period each year. Most ranchers in California, from small-scale producers (1 to 50 head) to larger producers (more than 5,000), participate in the integrated beef production system. Less than 1% of steers and heifers go from rangeland to meat processing. Like pastoralists, ranchers strategically move cattle around (and off) rangeland to optimize production within a variable climate. Ranchers indicate that their movements result from changes in forage quality and quantity and support their desire to manage for conservation objectives, including reducing fire fuels, controlling weeds, and managing for wildlife habitat. Inspection data, as well as direct observation, interviews, and surveys within the San Francisco Bay area, reveal the extent to which the region's ranchers rely on saleyards to facilitate the movement of cattle and integration of production systems. Saleyards and cattle buyers drive beef production efficiency by sorting, pricing, and moving cattle and matching them to feed resources in more intensive production systems. However, transactions lack traceability to inform policy and consumer choice. New data technologies like blockchain can provide traceability through integrated production-scapes and facilitate market development to support grazing landscapes and consumer choice.
Antimicrobial Usage Factors and Resistance Profiles of Shiga Toxin-Producing Escherichia coli in Backyard Production Systems From Central Chile
Authors: Pavez-Muñoz Erika , González Camilo , Fernández-Sanhueza Bastián , Sánchez Fernando , Escobar Beatriz , Ramos Romina , Fuenzalida Verónica , Galarce Nicolás , Arriagada Gabriel , Neira Víctor , Muñoz-Aguayo Jeannette , Flores-Figueroa Cristian , Johnson Timothy J. , Alegría-Morán Raúl
Published: January 2021

Shiga toxin-producing Escherichia coli (STEC) is a zoonotic pathogen and important cause of foodborne disease worldwide. Many animal species in backyard production systems (BPS) harbor STEC, systems characterized by low biosecurity and technification. No information is reported on STEC circulation, antimicrobial resistance (AMR) and potential drivers of antimicrobial usage in Chilean BPS, increasing the risk of maintenance and transmission of zoonotic pathogens and AMR generation. Thus, the aim of this study was to characterize phenotypic and genotypic AMR and to study the epidemiology of STEC isolated in BPS from Metropolitana region, Chile. A total of 85 BPS were sampled. Minimal inhibitory concentration and whole genome sequencing was assessed in 10 STEC strain isolated from BPS. All strains were cephalexin-resistant (100%, n = 10), and five strains were resistant to chloramphenicol (50%). The most frequent serotype was O113:H21 (40%), followed by O76:H19 (40%), O91:H14 (10%), and O130:H11 (10%). The stx1 type was detected in all isolated strains, while stx2 was only detected in two strains. The Stx subtype most frequently detected was stx1c (80%), followed by stx1a (20%), stx2b (10%), and stx2d (10%). All strains harbored chromosomal blaAmpC. Principal component analysis shows that BPS size, number of cattle, pet and horse, and elevation act as driver of antimicrobial usage. Logistic multivariable regression shows that recognition of diseases in animals (p = 0.038; OR = 9.382; 95% CI: 1.138–77.345), neighboring poultry and/or swine BPS (p = 0.006; OR = 10.564; 95% CI: 1.996–55.894), visit of Veterinary Officials (p = 0.010; OR = 76.178; 95% CI: 2.860–2029.315) and close contact between animal species in the BPS (p = 0.021; OR = 9.030; 95% CI: 1.385–58.888) increase significantly the risk of antimicrobial use in BPS. This is the first evidence of STEC strains circulating in BPS in Chile, exhibiting phenotypic AMR, representing a threat for animal and public health. Additionally, we identified factors acting as drivers for antimicrobial usage in BPS, highlighting the importance of integration of these populations into surveillance and education programs to tackle the potential development of antimicrobial resistance and therefore the risk for ecosystemic health.
Dietary Supplementation With Bacillus subtilis Promotes Growth and Gut Health of Weaned Piglets
Authors: Tian Zhilong , Wang Xiaodan , Duan Yehui , Zhao Yue , Zhang Wenming , Azad Md. Abul Kalam , Wang Zhanbin , Blachier Francois , Kong Xiangfeng
Published: January 2021

This study was conducted to investigate the effects of dietary supplementation with different types of Bacillus subtilis (B. subtilis) on the growth and gut health of weaned piglets. A total of 160 piglets were randomly assigned into four groups: control group (a basal diet), BS-A group (a basal diet supplemented with B. subtilis A at 1 × 106 CFU/g feed), BS-B group (a basal diet supplemented with B. subtilis B at 1 × 106 CFU/g feed), and BS-C group (a basal diet supplemented with B. subtilis C at 1 × 106 CFU/g feed). All groups had five replicates with eight piglets per replicate. On days 7, 21, and 42 of the trial, blood plasma and intestinal tissues and digesta samples were collected to determine plasma cytokine concentrations, intestinal morphology, gut microbiota community and metabolic activity, and the expression of genes related to gut physiology and metabolism. The results showed that dietary B. subtilis supplementation improved (P < 0.05) the body weight and average daily gain (in BS-B and BS-C groups) of weaned piglets and decreased (P < 0.05) the diarrhea rates (in BS-A, BS-B, and BS-C groups). In the intestinal morphology analysis, B. subtilis supplementation improved (P < 0.05) the size of villus height and villus height to crypt depth ratio in the ileum of weaned piglets. Firmicutes, Bacteroidetes, and Tenericutes were the most dominant microflora in piglets' colon whatever the trial group and time of analysis. Dietary BS-C supplementation increased (P < 0.05) the relative abundances of Anaerovibrio and Bulleidia and decreased (P < 0.05) the relative abundances of Clostridium and Coprococcus compared with the control group. In addition, dietary B. subtilis supplementation increased (P < 0.05) the indicators of intestinal health, including plasma levels of interleukin (IL)-2 and IL-10, as well as the colonic levels of short-chain fatty acids. Furthermore, dietary B. subtilis supplementation also up-regulated (P < 0.05) the expression of genes involved in metabolic pathways related to intestinal microbiota maturation. In conclusion, these findings suggest that a diet containing BS-B or BS-C can efficiently promote growth performance, decrease diarrhea incidence, and ameliorate several indicators of intestinal health through the modulation of gut microbiota composition and metabolic activity in weaned piglets.
Tackling the Threat of Rabies Reintroduction in Europe
Authors: Vega Santiago , Lorenzo-Rebenaque Laura , Marin Clara , Domingo Rosana , Fariñas Fernando
Published: January 2021

Rabies is one of the oldest, most important zoonoses worldwide due to its extreme and inevitably lethal nature, causing one death every 9 min worldwide. Recent reports have demonstrated that the Lyssavirus continues more alive than ever, despite the control carried out against the virus throughout Europe. In this context, this work reviews the main immunological implications, transmission risk factors and current prevention measures for virus control in Europe, and especially in Spain.
Effectiveness and Economic Viability of Johne's Disease (Paratuberculosis) Control Practices in Dairy Herds
Authors: Rasmussen Philip , Barkema Herman W. , Hall David C.
Published: January 2021

Johne's disease (JD or paratuberculosis) control programs have been established in many dairy-producing regions. However, the effectiveness (reduction of within-herd prevalence) and the relative economic impact as measured by, for example, the ratio of benefits to costs (BCR) across a comprehensive selection of regions and potential control practices require further investigation. Within a Markovian framework using region-specific economic variables, it was estimated that vaccination was the most promising type of JD control practice modeled, with dual-effect vaccines (reducing shedding and providing protective immunity) having BCRs between 1.48 and 2.13 in Canada, with a break-even period of between 6.17 and 7.61 years. Dual-effect vaccines were also estimated to yield BCRs greater than one in almost all major dairy-producing regions, with greater ratios in regions characterized by above-average farm-gate prices and annual production per cow. Testing and culling was comparably effective to a dual-effect vaccine at test sensitivities >70% but would remain economically unviable in almost all regions modeled.
Motivación laboral en una empresa de servicios de Lima, Perú
Authors: Nolazco Labajos Fernando Alexis , Bustamante Cieza Elvis Iván , Moreno Rodríguez Rosa Ysabel , Carhuancho Mendoza Irma Milagros
Published: January 2021

El talento humano es el recurso más importante y capaz de generar crecimiento en cualquier entidad. Por ello, compañías de todo tipo, tamaño, industria y ubicación direccionan  sus esfuerzos en satisfacer sus necesidades creando espacios donde superen sus expectativas y logren sus satisfacciones. En ese contexto, en la compañía en estudio, no se evidenció una estructura de reconocimientos, prevalece una falta de capacitación, de oportunidades, de crecimiento y  desarrollo. No existen estrategias para motivar al personal y/o espacios para el desarrollo de sus habilidades entre otras acciones. Por tal razón, el objetivo fue diagnosticar y determinar factores predominantes de la motivación laboral en los trabajadores de una empresa de servicios en Lima. El estudio se basó en un enfoque mixto, sintagma holístico, nivel comprensivo, método inductivo-deductivo,  la técnica fue la encuesta y entrevista apoyada de guía de entrevista, unidades informantes fueron treinta entre trabajadores y extrabajadores y tres entrevistados. Los resultados evidencian desmotivación respecto a reconocimientos, bonos de incentivos, recompensas pero adicionales al salario y al aspecto de autonomía. En tanto manifestaron sentirse motivados con la cooperación entre áreas; la afiliación con los dueños, la marca y la interrelación con los colegas generando un  ambiente de confianza. Se consideran como factores predominantes la flexibilidad de horario, el feedback, integración, el fomento de investigación y apertura a nuevas formas de solución de problemas
Systematic Review: Anesthetic Protocols and Management as Confounders in Rodent Blood Oxygen Level Dependent Functional Magnetic Resonance Imaging (BOLD fMRI)—Part B: Effects of Anesthetic Agents, Doses and Timing
Authors: Steiner Aline R. , Rousseau-Blass Frédérik , Schroeter Aileen , Hartnack Sonja , Bettschart-Wolfensberger Regula
Published: January 2021

In rodent models the use of functional magnetic resonance imaging (fMRI) under anesthesia is common. The anesthetic protocol might influence fMRI readouts either directly or via changes in physiological parameters. As long as those factors cannot be objectively quantified, the scientific validity of fMRI in rodents is impaired. In the present systematic review, literature analyzing in rats and mice the influence of anesthesia regimes and concurrent physiological functions on blood oxygen level dependent (BOLD) fMRI results was investigated. Studies from four databases that were searched were selected following pre-defined criteria. Two separate articles publish the results; the herewith presented article includes the analyses of 83 studies. Most studies found differences in BOLD fMRI readouts with different anesthesia drugs and dose rates, time points of imaging or when awake status was compared to anesthetized animals. To obtain scientifically valid, reproducible results from rodent fMRI studies, stable levels of anesthesia with agents suitable for the model under investigation as well as known and objectively quantifiable effects on readouts are, thus, mandatory. Further studies should establish dose ranges for standardized anesthetic protocols and determine time windows for imaging during which influence of anesthesia on readout is objectively quantifiable.
Functionally Graded AISI 316L and AISI H13 Manufactured by L-DED for Die and Mould Applications
Authors: Ostolaza Marta , Arrizubieta Jon Iñaki , Lamikiz Aitzol , Cortina Magdalena
Published: January 2021

Tooling in the die and mould industry is subjected to high-wear and high-temperature environments, which often leads to the premature failure of this high-added-value tooling. When severe damage occurs, an alternative to replacing the whole component consists of the repair by laser-directed energy deposition (L-DED). For that end, intermediate layers are commonly employed as buffer material, where introducing a functionally graded material (FGM) might be beneficial to avoid material incompatibilities and improve the overall performance of the tooling. In the present work, an FGM composed of gradient AISI 316L to AISI H13 has been manufactured, and its microstructure and hardness analysed. Firstly, cracking owing to the formation of brittle intermediate phases has been detected. Secondly, an increase of the hardness and a decrease of the corrosion resistance has been observed when transitioning from AISI 316L to AISI H13. Thirdly, despite the FGM composition evolving linearly, nonlinear material properties such as hardness and corrosion have been observed, which are conditioned by the microstructure formed during the L‑DED process and the nonlinear influence of the composition of steel on such properties. Consequently, nonlinear compositional gradients are recommended if linear mechanical properties are to be obtained in the case of steel FGMs.
Influence of Seal Cavity Leakage Flow on Compressor Performance Investigated with a Circumferentially Averaged Method
Authors: Liang Dong , Gui Xingmin , Jin Donghai
Published: January 2021

In order to investigate the effect of seal cavity leakage flow on a compressor’s performance and the interaction mechanism between the leakage flow and the main flow, a one-stage compressor with a cavity under the shrouded stator was numerically simulated using an inhouse circumferentially averaged through flow program. The leakage flow from the shrouded stator cavity was calculated simultaneously with main flow in an integrated manner. The results indicate that the seal cavity leakage flow has a significant impact on the overall performance of the compressor. For a leakage of 0.2% of incoming flow, the decrease in the total pressure ratio was 2% and the reduction of efficiency was 1.9 points. Spanwise distribution of the flow field variables of the shrouded stator shows that the leakage flow leads to an increased flow blockage near the hub, resulting in drop of stator performance, as well as a certain destructive effect on the flow field of the main passage.
Improved Delivery of Remedial Agents Using Surface Foam Spraying with Vertical Holes into Unsaturated Diesel-Contaminated Soil for Total Petroleum Hydrocarbon Removal
Authors: Bajagain Rishikesh , Gautam Prakash , Jeong Seung-Woo
Published: January 2021

Surface foam spraying technologies, employing natural infiltration processes, have recently been suggested to not disturb or mix contaminated soils. However, effective delivery of reactive remedial agents to the bottom area of a contaminated region using only natural infiltration processes can be a challenge. This study aimed to improve the delivery of remedial agents such as oxidants, microorganisms, and nutrients to all depths of 30 cm thick unsaturated diesel-contaminated soil using small vertical soil holes. Three vertical holes, occupying 0.8% of the total soil volume and 3% of the soil surface area, were made inside the 17.3 kg soil column. Persulfate oxidation foam and subsequent bioaugmentation foam spraying were applied for remediation of contaminated soil. Foam spraying with vertical soil holes improved the uniformity of distribution of remedial agents throughout the soil, as evidenced by the uniform pH, higher volumetric soil water content, and a microbial population of >107 CFU/g. Therefore, the total petroleum hydrocarbon (TPH) removal efficiency (88–90%) from bottom soils was enhanced compared to soil columns without holes (72–73%) and the control test (5–9%). The kinetic study revealed that relatively similar TPH biodegradation rates (0.054–0.057 d−1) can be obtained for all soil depths by using this new and simple approach.
Numerical Investigation on the Influence of the Streamlined Structures of the High-Speed Train’s Nose on Aerodynamic Performances
Authors: Sun Zhenxu , Yao Shuanbao , Wei Lianyi , Yao Yongfang , Yang Guowei
Published: January 2021

The structural design of the streamlined shape is the basis for high-speed train aerodynamic design. With use of the delayed detached-eddy simulation (DDES) method, the influence of four different structural types of the streamlined shape on aerodynamic performance and flow mechanism was investigated. These four designs were chosen elaborately, including a double-arch ellipsoid shape, a single-arch ellipsoid shape, a spindle shape with a front cowcatcher and a double-arch wide-flat shape. Two different running scenes, trains running in the open air or in crosswind conditions, were considered. Results reveal that when dealing with drag reduction of the whole train running in the open air, it needs to take into account how air resistance is distributed on both noses and then deal with them both rather than adjust only the head or the tail. An asymmetrical design is feasible with the head being a single-arch ellipsoid and the tail being a spindle with a front cowcatcher to achieve the minimum drag reduction. The single-arch ellipsoid design on both noses could aid in moderating the transverse amplitude of the side force on the tail resulting from the asymmetrical vortex structures in the flow field behind the tail. When crosswind is considered, the pressure distribution on the train surface becomes more disturbed, resulting in the increase of the side force and lift. The current study reveals that the double-arch wide-flat streamlined design helps to alleviate the side force and lift on both noses. The magnitude of side force on the head is 10 times as large as that on the tail while the lift on the head is slightly above that on the tail. Change of positions where flow separation takes place on the streamlined part is the main cause that leads to the opposite behaviors of pressure distribution on the head and on the tail. Under the influence of the ambient wind, flow separation occurs about distinct positions on the train surface and intricate vortices are generated at the leeward side, which add to the aerodynamic loads on the train in crosswind conditions. These results could help gain insight on choosing a most suitable streamlined shape under specific running conditions and acquiring a universal optimum nose shape as well.
Simulation of Crack Propagation in Reinforced Concrete Elements
Authors: Cramer Jonas , Javidmehr Sara , Empelmann Martin
Published: January 2021

The crack widths in reinforced concrete structures are affected by various influencing parameters, such as the tensile strength of the concrete, which is a highly variable parameter. While safe predictions of crack widths are possible with the existing models supplied in the code provisions accompanied by suitable safety factors, there are large discrepancies between the experimentally measured crack widths and the calculated values. Even within a uniaxial tensile test on reinforced concrete components, several crack parameters (spacings and widths) are usually measured. To further investigate the effects of scattering input parameters on the distribution of crack parameters, a crack propagation model (CPM) is developed, which is a powerful tool that can be used to observe sequential cracking of uniaxially loaded reinforced concrete components. In the present paper, the background of the scattering of the concrete tensile strength will be explained and the procedure for the simulation in the CPM, including the investigation of different bond–slip relationships, will be introduced. Finally, the CPM will be used to calculate experimental crack parameters and the distribution of the calculated crack parameters will be discussed.
Analysis of Dynamic Response of a Two Degrees of Freedom (2-DOF) Ball Bearing Nonlinear Model
Authors: Ambrożkiewicz Bartłomiej , Litak Grzegorz , Georgiadis Anthimos , Meier Nicolas , Gassner Alexander
Published: January 2021

Often the input values used in mathematical models for rolling bearings are in a wide range, i.e., very small values of deformation and damping are confronted with big values of stiffness in the governing equations, which leads to miscalculations. This paper presents a two degrees of freedom (2-DOF) dimensionless mathematical model for ball bearings describing a procedure, which helps to scale the problem and reveal the relationships between dimensionless terms and their influence on the system’s response. The derived mathematical model considers nonlinear features as stiffness, damping, and radial internal clearance referring to the Hertzian contact theory. Further, important features are also taken into account including an external load, the eccentricity of the shaft-bearing system, and shape errors on the raceway investigating variable dynamics of the ball bearing. Analysis of obtained responses with Fast Fourier Transform, phase plots, orbit plots, and recurrences provide a rich source of information about the dynamics of the system and it helped to find the transition between the periodic and chaotic response and how it affects the topology of RPs and recurrence quantificators.
Mathematical Model and FPGA Realization of a Multi-Stable Chaotic Dynamical System with a Closed Butterfly-Like Curve of Equilibrium Points
Authors: Sambas Aceng , Vaidyanathan Sundarapandian , Bonny Talal , Zhang Sen , Sukono  , Hidayat Yuyun , Gundara Gugun , Mamat Mustafa
Published: January 2021

This paper starts with a review of three-dimensional chaotic dynamical systems equipped with special curves of balance points. We also propose the mathematical model of a new three-dimensional chaotic system equipped with a closed butterfly-like curve of balance points. By performing a bifurcation study of the new system, we analyze intrinsic properties such as chaoticity, multi-stability, and transient chaos. Finally, we carry out a realization of the new multi-stable chaotic model using Field-Programmable Gate Array (FPGA).
Quaternion Processing Techniques for Color Synthesized NDT Thermography
Authors: Venegas Pablo , Usamentiaga Rubén , Perán Juan , Sáez de Ocáriz Idurre
Published: January 2021

Infrared thermography is a widely used technology that has been successfully applied to many and varied applications. These applications include the use as a non-destructive testing tool to assess the integrity state of materials. The current level of development of this application is high and its effectiveness is widely verified. There are application protocols and methodologies that have demonstrated a high capacity to extract relevant information from the captured thermal signals and guarantee the detection of anomalies in the inspected materials. However, there is still room for improvement in certain aspects, such as the increase of the detection capacity and the definition of a detailed characterization procedure of indications, that must be investigated further to reduce uncertainties and optimize this technology. In this work, an innovative thermographic data analysis methodology is proposed that extracts a greater amount of information from the recorded sequences by applying advanced processing techniques to the results. The extracted information is synthesized into three channels that may be represented through real color images and processed by quaternion algebra techniques to improve the detection level and facilitate the classification of defects. To validate the proposed methodology, synthetic data and actual experimental sequences have been analyzed. Seven different definitions of signal-to-noise ratio (SNR) have been used to assess the increment in the detection capacity, and a generalized application procedure has been proposed to extend their use to color images. The results verify the capacity of this methodology, showing significant increments in the SNR compared to conventional processing techniques in thermographic NDT.
Flatness-Based Aggressive Formation Tracking Control of Quadrotors with Finite-Time Estimated Feedforwards
Authors: Hou Zhicheng , Zhang Gong , Yang Wenlin , Wang Weijun , Han Changsoo
Published: January 2021

In this paper we address a decentralized neighbor-based formation tracking control of multiple quadrotors with leader–follower structure. Different from most of the existing work, the formation tracking controller is given in one loop without distinguishing the motion control and attitude control by means of the theory of flatness. In order to achieve an aggressive formation tracking, the high-order states of the neighbors motion are estimated by using a proposed extended finite-time observer for each quadrotor. Then the estimated motion states are used as feedforwards in the formation controller design. Simulation and experimental results show that the proposed formation controller improves the formation performance, i.e., the formation pattern of the quadrotors is better maintained than that using the formation controller without high-order feedforwards, when tracking an aggressive reference formation trajectory.
Cleaner Design and Production of Lightweight Aggregates (LWAs) to Use in Agronomic Application
Authors: Martínez-García Carmen , Andreola Fernanda , Lancellotti Isabella , Farías Romina D. , Cotes-Palomino Mª Teresa , Barbieri Luisa
Published: January 2021

This research focused on the obtainment of sustainable lightweight aggregates (LWAs) for agronomic application. The cleaner production is based on saving matter through the valorization of waste available in industry as a substitute of clays into the formulation of the lightweight aggregates (LWAs). Three different types of clays (white, black, and red) and alternative raw materials were blended. Cattle bone flour ash (CBA) and a fertilizer glass (FG) were used to introduce K and P into the mixture in amounts suitable for fertilizer application, and a sewage sludge from a brewery wastewater treatment plant was used as pore forming agent. For the production of the LWAs, we mixed different percentage of waste in two different clay mixtures, which were thermally treated at 1000 °C for 1 h. Technological parameters such as loose bulk and oven dry density, total porosity, water absorption capacity, pH, and electrical conductivity were determined to evaluate the potential use of LWAs as a growing media. Moreover, scanning electron microscopy (SEM) and mercury intrusion porosimetry (MIP) techniques were used, and leaching tests were performed to complete the samples’ characterization. The results indicated the potential for manufacturing high-quality LWAs for the agronomic field by using energy-saving and matter-processing involving low temperatures with respect to the conventional process.
Eicosanoid Content in Fetal Calf Serum Accounts for Reproducibility Challenges in Cell Culture
Authors: Niederstaetter Laura , Neuditschko Benjamin , Brunmair Julia , Janker Lukas , Bileck Andrea , Del Favero Giorgia , Gerner Christopher
Published: January 2021

Reproducibility issues regarding in vitro cell culture experiments are related to genetic fluctuations and batch-wise variations of biological materials such as fetal calf serum (FCS). Genome sequencing may control the former, while the latter may remain unrecognized. Using a U937 macrophage model for cell differentiation and inflammation, we investigated whether the formation of effector molecules was dependent on the FCS batch used for cultivation. High resolution mass spectrometry (HRMS) was used to identify FCS constituents and to explore their effects on cultured cells evaluating secreted cytokines, eicosanoids, and other inflammatory mediators. Remarkably, the FCS eicosanoid composition showed more batch-dependent variations than the protein composition. Efficient uptake of fatty acids from the medium by U937 macrophages and inflammation-induced release thereof was evidenced using C13-labelled arachidonic acid, highlighting rapid lipid metabolism. For functional testing, FCS batch-dependent nanomolar concentration differences of two selected eicosanoids, 5-HETE and 15-HETE, were balanced out by spiking. Culturing U937 cells at these defined conditions indeed resulted in significant proteome alterations indicating HETE-induced PPARγ activation, independently corroborated by HETE-induced formation of peroxisomes observed by high-resolution microscopy. In conclusion, the present data demonstrate that FCS-contained eicosanoids, subject to substantial batch-wise variation, may modulate cellular effector functions in cell culture experiments.
A Critical Review of Computational Creativity in Built Environment Design
Authors: Gu Ning , Amini Behbahani Peiman
Published: January 2021

Computational creativity in built environment (BE) design has been a subject of research interest in the discipline. This paper presents a critical review of various ways computational creativity has been and can be defined and approached in BE design. The paper examines a comprehensive body of contemporary literature on the topics of creativity, computational creativity, and their assessment to identify levels of computational creativity. The paper then proceeds to a further review of the implications of these levels specifically in BE design. The paper identifies four areas in BE design where computational creativity is relevant. In two areas—synthesis (generation) and analysis—there is considerable literature on lower levels of computational creativity. However, in two other areas—interfacing and communication—even the definition of computational creativity is not as defined and clear for the discipline, and most works only consider the role of computers as a supporting tool or medium. These open up future research opportunities for the discipline.
Prognostic and Predictive Values of Mismatch Repair Deficiency in Non-Metastatic Colorectal Cancer
Authors: Jin Zhaohui , Sinicrope Frank A.
Published: January 2021

Colorectal cancer (CRC) is the third most commonly diagnosed cancer worldwide. Universal MMR/MSI testing is standard of care for all patients with newly diagnosed CRC based on multi-society guidelines in the United States. Such testing is intended to identify patients with Lynch Syndrome due to a germline mutation in an MMR gene, but also detects those with sporadic dMMR/MSI-high CRCs. The prognostic utility of MMR/MSI status in non-metastatic colorectal cancer has been studied extensively, yet more limited data are available for its predictive utility. Results have not been entirely consistent due to potential stage-related differences and limited numbers of dMMR/MSI-H patients included in the studies. In this review, we summarize the current evidence for the prognostic and predictive value of dMMR/MSI-H in non-metastatic CRC, and discuss the use of this biomarker for patient management and treatment decisions in clinical practice.
Lipid Metabolism as a Targetable Metabolic Vulnerability in Colorectal Cancer
Authors: Zaytseva Yekaterina
Published: January 2021

Colorectal cancer (CRC), the second leading cause of cancer-related deaths according to the World Health Organization, remains a substantial public health problem worldwide [...]
Oncogenic Effects of HIV-1 Proteins, Mechanisms Behind
Authors: Isaguliants Maria , Bayurova Ekaterina , Avdoshina Darya , Kondrashova Alla , Chiodi Francesca , Palefsky Joel
Published: January 2021

People living with human immunodeficiency virus (HIV-1) are at increased risk of developing cancer, such as Kaposi sarcoma (KS), non-Hodgkin lymphoma (NHL), cervical cancer, and other cancers associated with chronic viral infections. Traditionally, this is linked to HIV-1-induced immune suppression with depletion of CD4+ T-helper cells, exhaustion of lymphopoiesis and lymphocyte dysfunction. However, the long-term successful implementation of antiretroviral therapy (ART) with an early start did not preclude the oncological complications, implying that HIV-1 and its antigens are directly involved in carcinogenesis and may exert their effects on the background of restored immune system even when present at extremely low levels. Experimental data indicate that HIV-1 virions and single viral antigens can enter a wide variety of cells, including epithelial. This review is focused on the effects of five viral proteins: envelope protein gp120, accessory protein negative factor Nef, matrix protein p17, transactivator of transcription Tat and reverse transcriptase RT. Gp120, Nef, p17, Tat, and RT cause oxidative stress, can be released from HIV-1-infected cells and are oncogenic. All five are in a position to affect “innocent” bystander cells, specifically, to cause the propagation of (pre)existing malignant and malignant transformation of normal epithelial cells, giving grounds to the direct carcinogenic effects of HIV-1.
Co-Promoted Ni Nanocatalysts Derived from NiCoAl-LDHs for Low Temperature CO2 Methanation
Authors: Zhang Fanying , Lu Bin , Sun Peiqin
Published: January 2021

Ni-based catalysts are prone to agglomeration and carbon deposition at high temperatures. Therefore, the development of Ni-based catalysts with high activities at low temperatures is a very urgent and challenging research topic. Herein, Ni-based nanocatalysts containing Co promoter with mosaic structure were prepared by reduction of NiCoAl-LDHs, and used for CO2 methanation. When the reaction temperature is 250 °C (0.1 MPa, GHSV = 30,000 mL·g−1·h−1), the conversion of CO2 on the NiCo0.5Al-R catalyst reaches 81%. However, under the same test conditions, the conversion of CO2 on the NiAl-R catalyst is only 26%. The low-temperature activity is significantly improved due to Co which can effectively control the size of the Ni particles, so that the catalyst contains more active sites. The CO2-TPD results show that the Co can also regulate the number of moderately basic sites in the catalyst, which is beneficial to increase the amount of CO2 adsorbed. More importantly, the NiCo0.5Al-R catalyst still maintains high catalytic performance after 92 h of continuous reaction. This is due to the confinement effect of the AlOx substrate inhibiting the agglomeration of Ni nanoparticles. The Ni-based catalysts with high performance at low temperature and high stability prepared by the method used have broad industrial application prospects.
Efficient Photocatalytic Degradation of Gaseous Benzene and Toluene over Novel Hybrid PIL@TiO2/m-GO Composites
Authors: Shajari Shayeste , Kowsari Elaheh , Seifvand Naemeh , Boorboor Ajdari Farshad , Chinnappan Amutha , Ramakrishna Seeram , Saianand Gopalan , Dashti Najafi Mohammad , Haddadi-Asl Vahid , Abdpour Soheil
Published: January 2021

In this work, the PIL (poly ionic liquid)@TiO2 composite was designed with two polymerized ionic liquid concentrations (low and high) and evaluated for pollutant degradation activity for benzene and toluene. The results showed that PIL (low)@TiO2 composite was more active than PIL (high)@TiO2 composites. The photodegradation rate of benzene and toluene pollutants by PIL (low)@TiO2 and PIL (high)@TiO2 composites was obtained as 86% and 74%, and 59% and 46%, respectively, under optimized conditions. The bandgap of TiO2 was markedly lowered (3.2 eV to 2.2 eV) due to the formation of PIL (low)@TiO2 composite. Besides, graphene oxide (GO) was used to grow the nano-photocatalysts’ specific surface area. The as-synthesized PIL (low)@TiO2@GO composite showed higher efficiency for benzene and toluene degradation which corresponds to 91% and 83%, respectively. The resultant novel hybrid photocatalyst (PIL@TiO2/m-GO) was prepared and appropriately characterized for their microstructural, morphology, and catalytic properties. Among the studied photocatalysts, the PIL (low)@TiO2@m-GO composite exhibits the highest activity in the degradation of benzene (97%) and toluene (97%). The ultimate bandgap of the composite reached 2.1 eV. Our results showed that the as-prepared composites hold an essential role for future considerations over organic pollutants.
MRGPRX2 Activation by Rocuronium: Insights from Studies with Human Skin Mast Cells and Missense Variants
Authors: Chompunud Na Ayudhya Chalatip , Amponnawarat Aetas , Roy Saptarshi , Oskeritzian Carole A. , Ali Hydar
Published: January 2021

Perioperative hypersensitivity (POH) to the neuromuscular blocking drug (NMBD) rocuronium was previously thought to be IgE and mast cell (MC)-mediated. However, the recent seminal observation that rocuronium induces degranulation in murine peritoneal MCs (PMCs) via Mas-related G protein-coupled receptor B2 (MrgprB2) led to the idea that POH to this drug involves the activation of MRGPRX2 (human ortholog of MrgprB2). Furthermore, based on the demonstration that a patient with POH to rocuronium displayed three missense mutations (M196I, L226P and L237P) in MRGPRX2’s transmembrane domains, it was proposed that this hypersensitivity reaction resulted from aberrant activation of this receptor. We found that rocuronium at 20 µg/mL caused degranulation in mouse PMCs via MrgprB2 but required at least 500 µg/mL to induce degranulation in human MCs via MRGPRX2. Furthermore, RBL-2H3 cells transiently expressing M196I, L226P and L237P variants did not display enhanced degranulation in response to rocuronium when compared to the wild-type receptor. These findings provide the first demonstration that rocuronium induces degranulation in human MCs via MRGPRX2. Furthermore, the important differences between MrgprB2 and MRGPRX2 and the inability of rocuronium to induce enhanced response in cells expressing MRGPRX2 variants suggest that the mechanism of its POH is more complex than previously thought.
PE_PGRS33, an Important Virulence Factor of Mycobacterium tuberculosis and Potential Target of Host Humoral Immune Response
Authors: Kramarska Eliza , Squeglia Flavia , De Maio Flavio , Delogu Giovanni , Berisio Rita
Published: January 2021

PE_PGRS proteins are surface antigens of Mycobacterium tuberculosis (Mtb) and a few other pathogenic mycobacteria. The PE_PGRS33 protein is among the most studied PE_PGRSs. It is known that the PE domain of PE_PGRS33 is required for the protein translocation through the mycobacterial cell wall, where the PGRS domain remains available for interaction with host receptors. Interaction with Toll like receptor 2 (TLR2) promotes secretion of inflammatory chemokines and cytokines, which are key in the immunopathogenesis of tuberculosis (TB). In this review, we briefly address some key challenges in the development of a TB vaccine and attempt to provide a rationale for the development of new vaccines aimed at fostering a humoral response against Mtb. Using PE_PGRS33 as a model for a surface-exposed antigen, we exploit the availability of current structural data using homology modeling to gather insights on the PGRS domain features. Our study suggests that the PGRS domain of PE_PGRS33 exposes four PGII sandwiches on the outer surface, which, we propose, are directly involved through their loops in the interactions with the host receptors and, as such, are promising targets for a vaccination strategy aimed at inducing a humoral response.
What Can You Learn about Apparent Surface Free Energy from the Hysteresis Approach?
Authors: Terpiłowski Konrad , Hołysz Lucyna , Chodkowski Michał , Clemente Guinarte David
Published: January 2021

The apparent surface free energy is one of the most important quantities in determining the surface properties of solids. So far, no method of measuring this energy has been found. The essence of contact angle measurements is problematic. Contact angles should be measured as proposed by Young, i.e., in equilibrium with the liquid vapors. This type of measurement is not possible because within a short time, the droplet in the closed chamber reaches equilibrium not only with vapors but also with the liquid film adsorbed on the tested surface. In this study, the surface free energy was determined for the plasma-activated polyoxymethylene (POM) polymer. Activation of the polymer with plasma leads to an increase in the value of the total apparent surface free energy. When using the energy calculations from the hysteresis based approach (CAH), it should be noted that the energy changes significantly when it is calculated from the contact angles of a polar liquid, whereas being calculated from the angles of a non-polar liquid, the surface activation with plasma changes its value slightly.
Diversity and Origin of the Central Mexican Alpine Flora
Authors: Steinmann Victor W. , Arredondo-Amezcua Libertad , Hernández-Cárdenas Rodrigo Alejandro , Ramírez-Amezcua Yocupitzia
Published: January 2021

Alpine vegetation is scarce in central Mexico (≈150 km2) and occurs on the 11 highest peaks of the Trans-Mexican Volcanic Belt (TMVB). Timberline occurs at (3700) 3900 m, and at 4750 m vascular plants cease to exist. The alpine vascular flora comprises 237 species from 46 families and 130 genera. Asteraceae (44), Poaceae (42), and Caryophyllaceae (21) possess 45% of the species; none of the remaining families have more than 10 species. Four species are strict endemics, and eight others are near endemics. Thirteen species are restricted to alpine vegetation but also occur outside the study area. Seventy-seven species are endemic to Mexico, 35 of which are endemic to the TMVB. In terms of biogeography, the strongest affinities are with Central or South America. Fifteen species are also native to the Old World. Size of the alpine area seems to not be the determining factor for its floristic diversity. Instead, the time since and extent of the last volcanic activity, in addition to the distance from other alpine islands, appear to be important factors affecting diversity. There is evidence for upward vegetational shifts having occurred during the last century.
The Hierarchical Classifier for COVID-19 Resistance Evaluation
Authors: Shakhovska Nataliya , Izonin Ivan , Melnykova Nataliia
Published: January 2021

Finding dependencies in the data requires the analysis of relations between dozens of parameters of the studied process and hundreds of possible sources of influence on this process. Dependencies are nondeterministic and therefore modeling requires the use of statistical methods for analyzing random processes. Part of the information is often hidden from observation or not monitored. That is why many difficulties have arisen in the process of analyzing the collected information. The paper aims to find frequent patterns and parameters affected by COVID-19. The novelty of the paper is hierarchical architecture comprises supervised and unsupervised methods. It allows the development of an ensemble of the methods based on k-means clustering and classification. The best classifiers from the ensemble are random forest with 500 trees and XGBoost. Classification for separated clusters gives us higher accuracy on 4% in comparison with dataset analysis. The proposed approach can be used also for personalized medicine decision support in other domains. The features selection allows us to analyze the following features with the highest impact on COVID-19: age, sex, blood group, had influenza.