bluuebunny/crossref_metadata_2025 huggingface dataset:
A mosaic pathogenic variant in MSH6 causes MSH6-deficient colorectal and endometrial cancer in a patient classified as suspected Lynch syndrome: a case report
Authors: Walker Romy , Clendenning Mark , Joo Jihoon E. , Xue Jessie , Mahmood Khalid , Georgeson Peter , Como Julia , Joseland Sharelle , Preston Susan G. , Chan James M. , Jenkins Mark A. , Rosty Christophe , Macrae Finlay A. , Palma Stephanie Di , Campbell Ainsley , Winship Ingrid M. , Buchanan Daniel D.
Published: April 2023

Germline pathogenic variants in the DNA mismatch (MMR) repair genes (Lynch syndrome) predispose to colorectal (CRC) and endometrial (EC) cancer. However, mosaic variants in the MMR genes have been rarely described. We identified a likely de novo mosaic MSH6:c.1135_1139del p.Arg379* pathogenic variant in a patient diagnosed with suspected Lynch syndrome/Lynch-like syndrome. The patient developed MSH6-deficient EC and CRC at 54 and 58 years of age, respectively, without a detectable germline MMR pathogenic variant. Multigene panel sequencing of tumor and blood-derived DNA identified an MSH6 somatic mutation (MSH6:c.1135_1139del p.Arg379*) common to both the EC and CRC, raising suspicion of mosaicism. A droplet digital polymerase chain reaction (ddPCR) assay detected the MSH6 variant at 5.34% frequency in normal colonic tissue, 3.49% in saliva and 1.64% in blood DNA, demonstrating the presence of the MSH6 variant in all three germ layers. This study highlights the utility of tumor sequencing to guide sensitive ddPCR testing to detect low-level mosaicism in the MMR genes. Further investigation of the prevalence of MMR mosaicism is needed to inform routine diagnostic approaches and genetic counselling.
Oncolytic Alphaherpesvirus and Myeloid-Tropic Cytomegalovirus Cooperatively Enhance Systemic Antitumor Responses
Authors: Jiang Haifei , Nace Rebecca , Ariail Emily , Ma Yejun , McGlinch Erin , Ferguson Coryn , Carrasco Talia Fernandez , Packiriswamy Nandakumar , Zhang Lianwen , Peng Kah Whye , Russell Stephen J.
Published: April 2023

Oncolytic virotherapy aims to activate host antitumor immunity, but the effectiveness of current intratumoral herpes-simplex virus (HSV) based monotherapy is limited. Here, to boost the potency of cancer cell targeting α-herpesviruses derived from HSV or Varicella-Zoster Virus (VZV), we coinjected them with myeloid cell-tropic β-herpesviruses derived from cytomegalovirus (CMV) which can powerfully modulate immune responses. Co-administered CMV was shown to promote intratumoral replication of HSV, increase local release of pro-inflammatory cytokines, inhibit adaptive antiviral immune responses, enhance systemic antitumor responses, and lead to superior control of both injected and contralateral tumors. CMV-encoded myeloid-tropic genes, NK evasins (i.e. the vMHC-I homolog), and the m02-m16 region (involved in downregulating classical MHC-I) were implicated in enhancing antitumor activities. CMV is a promising adjuvant to reshape and enhance the antitumor immune responses following α-herpesvirus therapy.
Three-dimensional volumetric investigation of Onodi cells: a multi-slice computer tomography study
Authors: Dieguez Flávia Limberg , De Rosa Catharina Simioni , Braz-Silva Paulo Henrique , Lopes Sérgio Lucio Pereira Castro , Costa Andre Luiz Ferreira
Published: April 2023

Purpose: This study aimed to investigate and evaluate the volumetric variation of OCs by using multi-slice computer tomography (MSCT) scans.
Methods: A retrospective review of MSCT scans of 79 subjects was performed, in which 40 were males, 39 were females, age range was 18 to 83 years and mean age was 39.6 years. The volumes of OCs on the right and left sides were measured by using ITK-SNAP open-source software with semi-automatic segmentation. The possible relationships among age, gender, contact with optic nerve, extension of the pneumatization of posterior ethmoid cells into the clinoid processes, mucous thickening in the anterior and posterior ethmoid cells and obliteration of the sphenoethmoidal complex were analysed with Pearson's correlation and chi-square tests according to type of compared data and logistic regression models (P < 0.05).
Results: It was observed that an increase of one unit in the volume of OCs also increases the chance of extension of pneumatization into the clinoid processes by 0.15% (P = 0.001). No significant correlations were identified between age, gender and volume of OCs.
Conclusion: The volume of OCs has effects on the extension of pneumatization into the clinoid processes.
Exploring the Impact of Recurrent Neural Networks on Image Classification and Optimizing Neural Configuration
Authors: Uddin Abdul Hasib
Published: April 2023

In this research, we delve into the unexplored potential of Long Short-term Memory (LSTM) and Gated Recurrent Unit (GRU) networks in the field of Computer Vision (CV). With the trend of Deep Learning methods shifting towards complex Neural Network architectures, researchers have acknowledged the efficacy of Dense and Convolutional Neural Networks (CNNs) in CV tasks, while Recurrent Neural Networks (RNNs) have been proven to be effective in Natural Language Processing (NLP) problems. Despite some recent developments in blending RNN with CNN for NLP, there have been very few attempts in applying RNN-based approaches in CV. To address this gap, we take a unique in order to develop LSTM and GRU models by tuning the number of layers and number of neurons in each layer. The models are then applied to multiple benchmark datasets (Fashion-MNIST, MNIST, EMNIST-Letters, EMNIST-Digits, Kuzushiji-MNIST, and Kuzushiji-49). Our experimental results indicate that LSTM and GRU networks significantly outperforms corresponding CNN and Dense networks with optimal number of parameters. Our findings suggest that RNN (LSTM and GRU) can be valuable alternatives to CNN and Dense networks for image classification problems in CV. This study presents a new direction for further research in the field and highlights the potential of LSTM and GRU networks in CV tasks.
Optimal water allocation integrated with water supply, replenishment, and spill in the in-series reservoir based on an improved decomposition and dynamic programming aggregation method
Authors: Xu Zuping , Gong Zhihao , Cheng Haomiao , Cheng Jilin
Published: April 2023

In humid regions with the monsoon climate, seasonal water shortages and water spills occur alternately because of uneven temporal and spatial distributions of water resources. An optimization model for the in-series reservoir (ISR) with replenishment pumping stations was developed to obtain the minimum annual sum of water shortage and systematically considered the reservoir operation rule of water spill and replenishment. This model features multiple dimensions; dynamic programming (DP) may cause a ‘curse of dimensions’, while the decomposition-coordination method has difficulty in judging logic conditions in the reservoir operation rules. So, an improved decomposition and DP aggregation (DDPA) method was proposed. The proposed model and the method were applied to a real case in the humid region of southern China. Compared to a conventional scheduling method, the water supply was increased by 0.8% and replenishment was reduced by 2.5%. Moreover, a comparison between DDPA and six heuristic algorithms was discussed. All heuristic algorithms' objective function values only obtained local optimal solutions, and the water shortage of the system was 0.12–20.5%. The obtained results demonstrated that DDPA was the better choice for highly complex multi-reservoir systems. The proposed optimization algorithm enriched the optimization theory of multi-dimensional and multi-variable complex systems.
Norms and Institutions of International Economic Relations in the Indo-Pacific. An Epitaph for International Economic Law?
Authors: Menkes Jerzy
Published: April 2023

The paper presents the conclusions of an analysis of the challenges globally facing the liberal economy. The author examines the internal (its contestation in “Western” countries) and external (abuse of the law and trust by WTO members not respecting the goals and principles of the organization) challenges. Regarding external challenges, the focus here is on the initiative to build a new order in the Indo-Pacific region. The thesis that as a consequence of the “West’s” assessment that the international economic legal regime is an ineffective tool for regulating the LIEO and its willingness to sustain the LIEO, the West decided to replace the legal regime with another one, and was positively verified. 

The study closes with a forecast of the evolution of the regional economic order and its links to the world economy.
Molecular Mechanism of Caulis Spatholobi in the Treatment of Chronic
Myeloid Leukemia based on Network Pharmacology and Experimental
Verification
Authors: Wu Yanchun , Lian Fangfang , Chen Hongxia , Zhang Chaoyu , Wei Linli , Tian Hui
Published: April 2023

Background:

Caulis Spatholobiis one of the necessary Chinese herbal medicines for
hematologists in the treatment of malignant tumors, but its potential targets and molecular mechanisms
need further exploration.

Objective:

This study aimed to predict the relevant targets of the treatment of chronic myeloid leukemia
(CML) with Caulis Spatholobi by applying the network pharmacology method, and in vitro cell
experiments were conducted to verify the mechanism of Caulis Spatholobi in the treatment of CML.

Methods:

TCMSP, ETCM, Genecards, and GisGeNET databases were used to obtain relevant
targets of Caulis Spatholobi in the treatment of CML. Go and KEGG analyses were performed
using the David database. Using Cytoscape 3.7.2, the "active compounds-targets-pathways" network
was constructed. Further validation was carried out by pharmacological experiments in vitro.
The proliferation and apoptosis of K562 cells were observed by the MTT method and Hoechst
33242 fluorescence staining method. The predicted targets and their related signal pathways were
verified by western blotting.

Results:

In this study, 18 active compounds and 43 potential targets were obtained. The results of
the MTT method showed that compared with the normal control group, 62.5-500 μg/mL alcohol
extract of Caulis Spatholobi had an obvious inhibitory effect on K562 and the IC50 value was less
than 100 μg/mL. The Hoechst 33242 fluorescence staining method showed that the alcohol extract
of Caulis Spatholobi could promote apoptosis. The results of western blotting showed that compared
with the normal control group, the expressions of Bax and Caspase-3 proteins in the 62.5 and
125 μg/mL alcohol extract of Caulis Spatholobi groups were significantly up-regulated (p < 0.05).
The expression of Bcl-2 in the 125 μg/mL alcohol extract of the Caulis Spatholobi group was significantly
down-regulated (p < 0.01), and the expression of Bcl-2 in the 62.5 and 31.25 μg/mL alcohol
extract of Caulis Spatholobi groups was also significantly down-regulated (p < 0.05). It showed that
the ethanol extract of Caulis Spatholobus could promote apoptosis by up-regulating the expression of
Bax and caspase-3 and down-regulating the expression of the Bcl-2 protein.

Conclusion:

The treatment of Caulis Spatholobi for CML has the characteristics of multi-targets
and multi-pathways. The results of in vitro pharmacological experiments demonstrated that its
mechanism of action might be based on the expression of key target proteins, such as Caspase-3,
Bcl-2, and Bax, thereby inhibiting cell proliferation and promoting cell apoptosis, which provides
a scientific basis for the treatment of CML.
Self-nano-emulsifying Drug Delivery Systems of Atorvastatin Calcium
Liquid Filled in Hard Shell Capsules for Improved Oral Bioavailability in
Rabbits
Authors: Yadav Khushwant S. , Arora Shivani , Yasaswi P. Soma , Nirale Prabhuti , Solanki Anita , Bhat Jnanadeva
Published: April 2023

Aims and Objectives:

Atorvastatin calcium (ATR) is a BCS class II drug showing poor
bioavailability due to limited aqueous solubility. In the present study, a self-nano-emulsifying drug
delivery system (SNEDDS) was developed and formulated as a liquid filled in a hard shell capsule
to improve the bioavailability of ATR.

Methods:

Different oils were screened through the saturated stability method, and the amount of
ATR solubilized in the respective oils was analysed through HPLC at 245nm. A ternary phase diagram
was plotted to obtain the optimized ratio of oil, surfactant, and co-surfactant to formulate
SNEDDS. The prepared ATR SNEDDS was filled into hard shell capsules, band sealed, and subjected
to various evaluations like disintegration time, self-emulsification time, precipitation time
assessment, globule size analysis and zeta potential. Then the in vitro dissolution studies were carried
out. The optimized SNEDDS formulation was filled in a hard shell capsule, and in vivo studies
were performed on rabbits to compare the pharmacokinetic parameters with the marketed formulation
and pure ATR.

Results:

Capmul MCM as the oil component showed five-fold solubility of ATR and was selected
for the preparation of ATR-SNEDDS. The SNEDDS formulation showed an entrapment efficiency
of 89.76±4.1% ATR with a globule size of 385±1.9 nm and an emulsification time of 5 seconds. It
was established from the study that liquid ATR-SNEDDS had relative bioavailability enhanced by
1.7 times in comparison to the marketed formulations (Lipvas) and 4.8 times with respect to pure
ATR.

Conclusion:

From the study, it was concluded that the bioavailability of ATR was enhanced by
formulating ATR as Liquid SNEDDS filled in hard shell capsules.
Transcriptional Factors Mediated Reprogramming to Pluripotency
Authors: Fatima Nazira , Saif Ur Rahman Muhammad , Qasim Muhammad , Ali Ashfaq Usman , Ahmed Uzair , Masoud Muhammad Shareef
Published: April 2023

A unique kind of pluripotent cell, i.e., Induced pluripotent stem cells (iPSCs), now being targeted
for iPSC synthesis, are produced by reprogramming animal and human differentiated cells (with no
change in genetic makeup for the sake of high efficacy iPSCs formation). The conversion of specific cells
to iPSCs has revolutionized stem cell research by making pluripotent cells more controllable for regenerative
therapy. For the past 15 years, somatic cell reprogramming to pluripotency with force expression of
specified factors has been a fascinating field of biomedical study. For that technological primary viewpoint
reprogramming method, a cocktail of four transcription factors (TF) has required: Kruppel-like factor
4 (KLF4), four-octamer binding protein 34 (OCT3/4), MYC and SOX2 (together referred to as
OSKM) and host cells. IPS cells have great potential for future tissue replacement treatments because of
their ability to self-renew and specialize in all adult cell types, although factor-mediated reprogramming
mechanisms are still poorly understood medically. This technique has dramatically improved performance
and efficiency, making it more useful in drug discovery, disease remodeling, and regenerative medicine.
Moreover, in these four TF cocktails, more than 30 reprogramming combinations were proposed, but for
reprogramming effectiveness, only a few numbers have been demonstrated for the somatic cells of humans
and mice. Stoichiometry, a combination of reprogramming agents and chromatin remodeling compounds,
impacts kinetics, quality, and efficiency in stem cell research.
Menstrual Blood-Derived Mesenchymal Stem Cell Therapy for Severe
COVID-19 Patients
Authors: Heidari Fezzeh , Heidari Reza , Sabet Mehrdad Nasrollahzadeh , Hamidieh Amir Ali , Saltanatpour Zohreh
Published: April 2023

The coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome
coronavirus (SARS-CoV-2), was declared a global pandemic in March 2020 and resulted in more than 6
million deaths worldwide to date. Although several vaccines were produced against COVID-19 and many
therapeutic protocols were developed for the management of this respiratory infection, COVID-19 pandemic
has still remained an unresolved problem with the emergence of new variants of SARS-CoV-2,
especially vaccine-resistant variants. Probably, end of the COVID-19 needs effective and certain treatments
which were undiscovered to date. According to immunomodulatory and regenerative properties,
mesenchymal stem cells (MSCs) have been considered a therapeutic approach to suppressing cytokine
storm caused by SARS-CoV-2 and the treatmet of severe COVID-19. Following intravenous (IV) infusion
of MSCs, cells entrap in the lung, guard alveolar epithelial cells, suppress pulmonary fibrosis and
improve lung dysfunction. The human menstrual blood-derived stem cells (hMenSCs) as a novel source
of MSCs are collected by noninvasive, painless, and easy way without ethical issues. MenScs are an
abundant and cheap source with a high proliferation rate and differentiation ability into multiple cell lineages.
Regarding immunomodulatory and anti-inflammatory properties, regenerative ability and low immunogenicity,
these cells exhibit great potential in the treatment of various diseases. Some clinical trial
studies have begun using MenSCs to treat severe COVID-19. According to these trials, MenSC therapy
showed promising and encouraging results in treating severe COVID-19. We reviewed published clinical
trials and summarized the effects of MenSC therapy on severe COVID-19 with a focus on clinical and
laboratory data, immune and inflammatory factors and concluded the advantages and possible risks of this
procedure.
Role of Lipocalin-2 in N1/N2 Neutrophil Polarization After Stroke
Authors: Guo Zhiliang , Xu Guoli , Xu Jiaping , Huang Yaqian , Liu Chunfeng , Cao Yongjun
Published: April 2023

Background:

Neutrophils and Lipocalin-2 (LCN2) play pivotal roles in cerebral ischemiareperfusion
(I/R) injury. However, their contribution is not fully clarified.

Objective:

This study aimed to explore the role of LCN2 and its association with neutrophil polarization
in I/R injury.

Methods:

A mouse model of middle cerebral artery occlusion (MCAO) was used to induce cerebral
ischemia. LCN2mAb was administered 1 h and Anti-Ly6G was administered for 3d before MCAO.
The role of LCN2 in the polarity transition of neutrophils was explored using an in vitro HL-60 cell
model.

Results:

LCN2mAb pretreatment had neuroprotective effects in mice. The expression of Ly6G was
not significantly different, but the expression of N2 neutrophils was increased. In the in vitro study,
LCN2mAb-treated N1-HL-60 cells induced N2-HL-60 polarization.

Conclusion:

LCN2 may affect the prognosis of ischemic stroke by mediating neutrophil polarization.
LINC01176 Hinders Thyroid Cancer Progression by Sponging miR-146b-5p
to Enhance SGIP1
Authors: Yan Zhaodan , Yan Chong
Published: April 2023

Background:

Long non-coding RNA (lncRNAs) plays a crucial role in tumor pathogen-esis. However, the function of most of these genes remains unclear.

Aims:

In the present study, we aimed to unveil LINC01176’s role in thyroid cancer.

Methods:

Western blotting and qRT-PCR were applied for the analysis of the expressions of LINC01176, miR-146b-5p, and SH3GL interacting endocytic adaptor 1 (SGIP1). Proliferative and migratory capabilities were assessed using the CCK-8 assay and wound-healing experiments, re-spectively. Apoptosis of the cells was studied by quantifying the apoptosis-related markers Bcl-2 and Bax by western blotting. Animal models were established using nude mice to determine the role of LINC01176 in tumorigenesis. MiR-146b-5p’s putative binding to LINC01176 and SGIP1 was validated using dual-luciferase reporter and RIP analyses.

Results:

LINC01176 expression was downregulated in the thyroid cancer cell lines and tissues. LINC01176 overexpression represses cancer cell proliferation and migration but induces apoptosis. Elevated LINC01176 expression hampers tumorigenesis in animal models. LINC01176 targeted miR-146b-5p and negatively regulated its expression. Enrichment of miR-146b-5p counteracted the functional effects of LINC01176 overexpression. Additionally, miR-146b-5p interacted with SGIP1 and negatively regulated its expression. Thus, miR-146b-5p attenuates the anti-cancer ef-fects of SGIP1.

Conclusion:

LINC01176 negatively regulates the expression miR-146b-5p and upregulates SGIP1 expression. Hence, LINC01176 blocks the malignant progression of thyroid cancer.
Dysregulation of miR-551b-5p and SETD2 Predicts Poor Prognosis and
Promotes Migration and Invasion of Thyroid Cancers
Authors: Dong Anbing , Gao Ming , Zheng Xiangqian , Ruan Xianhui
Published: April 2023

Objective::

This study was to investigate the clinical significance of miR-551b-5p and SETD2 in thyroid cancers (TC) and their effects on the biological function of TC cells

Methods::

The expression level of miR-551b-5p and SETD2 in tumor/nontumor tissues and TC cell lines was measured by quantitative real-time polymerase chain reaction (RT-qPCR). Subsequently, the relationship between miR-551b-5p or SETD2 expression and the clinicopathological feature was detected by Chi-square analysis. Kaplan-Meier and multivariate Cox regression analyses were used to assess their prognostic values. Finally, the regulatory effects of miR-551b-5p and SETD2 on the proliferation, migration and invasion ability of TC cells were detected by CCK-8 and Transwell assays.

Results::

Compared with non-tumor groups, the expression of miR-551b-5p was significantly increased in patients' tissues and TC cell lines, while SETD2 mRNA expression was decreased. Patients with up-regulated miR-551b-5p or downregulated SETD2 mRNA in TC showed more positive lymph node metastasis and advanced TNM stage. High miR-551b-5p expression level and low SETD2 mRNA level were related to poor survival rate. miR-551b-5p and SETD2 might be potential prognostic biomarkers for TC. miR-551b-5p knockdown can inhibit cell proliferation, migration and invasion by targeting SETD2

Conclusion::

miR-551b-5p and SETD2 may be valuable prognostic biomarkers and new therapeutic targets for TC.
Degree of Concordance Between Cone Beam Computerized Tomography (Cbct) And 2d Digital Radiography System Used for Caries Diagnosis: An Observational Study
Authors: Pulido Gisela , Arango Maria , Adriana Herrera , Gaviria Ángela , Ocampo Juan , Gutiérrez Bruno , Martínez Carlos , Sinisterra Gustavo , Valencia Carlos
Published: April 2023

Background:

Caries is a disease with a high prevalence, which may be due to the lack of adequate methods for its early diagnosis, becomes difficult to detect initial caries lesions in posterior teeth due to the occlusal anatomy of these and because on many occasions they start in the interproximal zone below the contact areas.

Different techniques have been used for the diagnosis of caries, such as visual-tactile inspection, radiography, tomography, and transillumination, but none of them seems to have complete sensitivity or specificity.

Aim:

This study aims to establish the degree of agreement between radiographic diagnosis with 2D intraoral digital radiography in relation to cone beam computed tomography in extracted carious and healthy posterior teeth, practicing the CariesCare International ™ practical guide as a reference.

Materials and Methods:

A total number of 117 teeth with or without carious lesions were selected. Digital images of all teeth were obtained from 2D digital radiographs (instrumentarium ™ films) obtained with Kavo focus equipment and CBCT scanner I-CAT ™ model 1719, ISI (imaging science international), Pennsylvania (blue sky plan 4 software).

Observers evaluated the images for the detection of carious lesions. The sensitivity and specificity ratio between the systems was determined using the area under the curve statistical tool ROC (Receiver Operating Characteristic).

For the statistical analysis, the statistical program STATA IC/16 was used, in which the calculation of absolute and relative frequencies for each category of CariesCare international in CBCT and radiography was performed, in addition to the Friedman and post-hoc Wilcoxon test by pairs; the distribution according to the classification of CariesCare international was contrasted between the CBCT slices. The calculation of weighted Kappa and dichotomous Kappa and the calculation of validity (sensitivity and specificity) and prediction (predictive values and predictive ratios) were performed.

The distribution according to CariesCare international classification was contrasted between the CBCT slices.

Results:

The results of the present study confirm that diagnosis of caries with the 2D digital radiographic method, initial caries lesions tend to go undetected relative to CBCT.

Conclusion:

2D digital radiographs showed low sensitivity for caries detection and high specificity relative to CBCT for caries lesions in vitro. Diagnosis with 2D digital Rx is considered non-discriminative in identifying initial caries lesions.
Obtaining TiO2 Nanostructures by Electrospinning and Analysis of Absorbance in the UVA Spectrum for Photocatalytic Application
Authors: Soares Luana Góes , Alves Annelise Kopp
Published: April 2023

The synthesis of fibers by electrospinning allows for obtaining nanostructures of one-dimensional materials with good flexibility, optical and catalytic properties, high surface area and porosity. They can be used in applications such as: catalysts, solar cells, fuel cells, membranes, and hydrogen batteries, among others. Here we emphasize that few reports in the literature describe the existing synergism between the optical and photocatalytic properties of TiO2, and the influence of this synergism on the formation of oxygen vacancies, which are responsible for the increase of the photocatalytic activity of this photocatalyst. Therefore, in this work we synthesize TiO2 nanofibers by electrospinning. These nanofibers were thermally treated between 650°C and 800°C, and characterized according to: morphology by scanning electron microscopy (SEM), crystalline phases by X-ray diffraction (XRD), photoactivity through degradation tests of 125 mL of a sample of 20 ppm methyl orange dye solution and by colorimetry. The results point to the TiO2 sample heat-treated at 650°C being more effective in the dye degradation and the reflection of different colors, possibly due to the combination of the chemical and physical properties of TiO2, which at the same time degrade the dye and express variations between different colors.
Use of Artificial Intelligence in Skin Aging
Authors: Jiminez Victoria S. , Chung Minh , Saleem Mohammad , Yusuf Nabiha
Published: April 2023

Skin aging is a complex process that involves several extrinsic and intrinsic factors and skin health is an indicator of the well-being of an individual. In recent years, there have been numerous developments using computerized systems to aid in finding solutions and treatments to skin aging processes. Tools like artificial intelligence (AI) can aid in finding solutions and treatments for skin aging. AI can also help in monitoring or identifying early signs of skin aging. Within the field of skin aging, several innovations utilize AI to provide better patient care. There is a gap in knowledge within this field concerning current and future directions concerning skin aging and AI. In this review, we aim to highlight current and prospective applications of AI in skin aging and provide insights into future modalities in this field. Models for AI can serve to increase patient participation in skin-care decisions and eventually enhance the patient-provider experience.
Risk Assessment for Logistics Applications in Cloud Migration
Authors: Maniah Maniah , Soewito Benfano , Gaol Ford Lumban , Abdurachman Edi
Published: April 2023

The increase in the number of cloud data centers is due to an increase in the number of companies migrating to cloud computing. There are many advantages that companies get when migrating to the cloud, but there are also many disadvantages. Multitenancy security and privacy are important challenges for cloud migration users. This study proposes a way to assess the risks that may arise in the cloud migration process for logistics business applications. The research method used is semi-quantitative with a 3-phase approach, namely before migration, during migration, and after migration by considering the criteria for risk aspects and environmental aspects that will have an impact on the company, so that companies can make risk mitigation plans. The results of this study identified 11 (eleven) threats in the cloud that occupy the top ranking and identify as many as 17 (seventeen) indicators obtained from the identification of indicators in the previous model or framework used to assess risks in logistics business applications that will be implemented. migrated to the cloud. Based on the experimental results in this study, the application risk value during migration and after migration has a higher value than before migration, and the risk value during migration are higher than the risk value after migration.
Perancangan dan Pengukuran Topi COVID-19 Reminder dengan Sensor Ultrasonik HC-SR04
Authors: Setyanto Barry Nur , Pravitasari Filly , Budiastuti Pramudita , Ghozali Fanani Arief
Published: April 2023

The development of ultrasonic sensor technology used for suitable equipment is widely carried out, such as distance sticks for the blind, floor cleaning robots, automatic garbage boxes, height measuring instruments, water volume detectors, automatic dispensers, automatic hand sanitisers, etc. During the COVID-19 pandemic, a product that can be used to limit the distance between humans is needed, so this research is focused on products for measuring distances with ultrasonic sensors. The research design method used is research and development, using the Borg and Gall approach, with step information collecting, planning, development, testing, final product and implementation. The results of this research product are designed using Arduino nano v3 hardware, battery, buzzer, and ultrasonic sensor HC-SR04. Product design was registered with Industrial Design Intellectual Property Rights with registration number A00202102408. The results of measuring the quality of the ultrasonic sensor for measuring distances on flat objects show an average error percentage of 2.94% from 10 cm to 300 cm with a distance range of 10 cm. Tests on obstacle objects (humans) at an angle of zero degrees and 15 degrees showed the best farthest measuring distance at a distance of 150 cm. So 150 cm is the best distance to apply to the hat COVID-19 reminder. The development results show that the product is ready to continue in production and sales.
Identification of Sea Surface Temperature Anomaly during Earthquake in Southern Java Island using Google Earth Engine Datasets
Authors: Rusydi Alfi Nur , Masitoh Ferryati
Published: April 2023

The certainty of Sea Surface Temperature (SST) anomaly during earthquakes in Java Island was still not clear identified. This research tried to identify any possibilities of SST anomaly during earthquake in Southern Java Island. It used Google Earth Engine datasets including daily NOAA Climate Data Record (CDR) - Woods Hole Oceanographic Institution (WHOI): Sea Surface Temperature Version 2 which has resolution of 0.25 arc degrees for 20 years and processed them to depict SST trends in pre-earthquake and post-earthquake situation. The statistic test of t-Test Paired Two Sample was also applied to certain the significant difference of SST in both situations. SST fluctuation during the earthquake was still inconsistent and unclear to consider as an anomaly. There were other complicated factors excluding earthquake to influence SST fluctuation. The earthquake did not trigger directly to fluctuate SST, even not to make it being an anomaly.
Informal Space Utilization Shifting post-Urban Revitalizationin the Semarang Old City
Authors: Dewi Santy Paulla , Susanti Retno , Wungo Grandy Lorenessa
Published: April 2023

The Semarang Old City revitalization has changed its physical appearance significantly, from a slum and crowded area to a more organized, pleasant, and attractive one. The revitalization is one of the Mayor's missions after being reelected in 2015 to enhance the Old City’s image as a prominent historical tourist destination. The government established a regulation prohibiting informal activities in the Old City. Informality is considered an interference in urban areas, including spaces that accommodate informal activities through illegal acquisition. However, informal activities persist in the Old City, shifting the physical setting and transforming the spatial utilization. Therefore, this study aimed to examine the transformation of informal spaces in the Old City after revitalization. Quantitative methods were used by distributing questionnaires to informal activity workers and interviews with the Old City manager. The results showed spatial utilization and relation transformations, a close relationship between informal and formal activities, and no conflict regarding spatial utilization. However, the relationship between formal and informal spaces changed after the revitalization. Formal activities prefer clear spatial boundaries, making informal space utilization and pattern more dynamic.
Spatial Distribution Pattern of Hypertension: Case of Jakarta, Indonesia
Authors: Makful Martya Rahmaniati , Isabel Yohana Septianty , Adrian Verry
Published: April 2023

Hypertension is one type of Non-communicable Disease (NCD) that is a burden on the government in disease control every year. Hypertension is caused by various risk factors. Most of the risk factors for hypertension are lifestyles that can be changed. This study aims to determine the pattern of distribution of hypertension cases based on risk factors, social factors, health care facilities. The spatial approach was used to determine the spatial relationship between hypertension risk factors and hypertension cases in the Jakarta province. The spatial approach was used to determine the spatial relationship between hypertension risk factors and hypertension cases in the Jakarta province. The results showed that the screening program variable had a spreading pattern with a negative spatial relationship and there was a spatial interaction between the screening program variables and hypertension cases. Improving the quality and quantity of Non-communicable Disease Integrated Assistance Post activities of local health centers, which are the front line in preventive and promotive activities is expected to be the key to successful control of hypertension cases in the Jakarta.
Spatial and Ecological Approach on Marble Mining Land in Tulungagung Regency-Indonesia: Is it Suitable as an Assessment of Disaster Mitigation Efforts?
Authors: Khusna Nur Isroatul , Sumarmi Sumarmi , Bachri Syamsul , Astina I Komang , Aristin Nevy Farista
Published: April 2023

Indonesia has a karst region abundant in high-quality marble mining products but is also prone to disasters. Over the years, this area has experienced numerous natural disasters, thereby leading to the need for a land suitability assessment. This is a descriptive qualitative and quantitative research with data collected from field observations, laboratory tests, interviews, and documents. Four places in the center and adjacent areas of marble producers were selected for the research sample. The data collected were analyzed using the spatial and ecological approach, fishbone, tabulation, percentage, and SWOT analysis. The result showed that numerous land mismatches contribute to the occurrence of natural catastrophes, such as improper vegetation kinds, population settlement patterns, excessive exploitation, and unfriendly human activities. This study recommends a variety of measures to improve the mining area.
Spatial and Temporal Analysis of 2019 Novel Coronavirus (2019-Ncov) Cases in Selangor, Malaysia
Authors: Abdul Gani Norezuan Abdul , Che Dom Nazri , Dapari Rahmat , Precha Nopadol
Published: April 2023

The COVID-19 pandemic continues to wreak devastation on public health systems worldwide, particularly in Selangor, Malaysia, COVID-19 was reported from October 2020 to October 2021 at prevalent rate. In order to control and prevent the spread of this pandemic, which is already underway, there is need to comprehend the spatial dimension of this disease. Therefore, the purpose of this study was to describe the patterns of COVID-19 virus transmission in the state of Selangor. Methods: Using a Geographic Information System (GIS), and the Moran’s Index (MI), spatial distribution of COVID-19 across the entire mukim was mapped and spatial statistical analysis was carried out with indications of local spatial correlations. Results: The finding revealed that the clusters were concentrated in the western and southern regions (Global Moran's I = 0.468, p = 0.05, Z = 7.01) of the state of Selangor, thus, this research provides important information on the regional distribution and temporal dynamics of COVID-19. Conclusion: An assessment of COVID-19's geographic spread can help enhance health care programs and resource allocation in Malaysia, specifically Selangor where the COVID-19 is pandemic.
The Temporal and Spatial Analysis of Corona Pandemic in Jordan using the Geographic Information System: An Applied Geographical Study
Authors: Taran Ayed , Alfanatseh AbedAlhameed , Rawashdeh Shatha , Almayouf Faisal
Published: April 2023

The coronavirus disease which results from severe acute respiratory syndrome (SARS-COV-2), is considered a global challenge affecting millions of people and leading to a global increase in mortality, including in Jordan. Therefore, this study aims to analyze the temporal and spatial patterns of the prevalence and outbreak of coronavirus in Jordan during six periods, from 1, October 2020 until 31, March 2021 by applying geographical information systems. The Moran coefficient was applied in addition to the G* test and location quotient (LQ). The results showed the overall pattern for the distribution of cases affected by the virus was random since most governorates' experience increased the focus and prevalence of the pandemic. Furthermore, four hot spots were revealed, namely Amman, Irbid, Zarqa, and Balqa'. This study introduced new insights into the statistical analysis of the distribution and prevalence of coronavirus in Jordan using geographical information systems. This will help planners and decision-makers to predict the dynamics of the temporal and spatial transfer of the virus in the future. It will also explain the current situation to set the appropriate policies or measures to face the pandemic, as well as reduce its prevalence. Therefore, monitoring, evaluating, and planning the usage of geospatial analysis are essential for controlling the spread of COVID-19 in the country.
Kasus Stunting sebagai Salah Satu Tantangan Pembangunan Berkelanjutan di Kota Salatiga
Authors: Safina Shafa Widad , Nugraha Adithya Thafari , Nuraini Afifah Neneng , Taradipa Farrade Dizna , Setiadi Irza Nadila Alifia , Rindika Lisa , Chairani Maulidia Savira , Aditya Muhammad Yuda , Dhafita Nadya Anindya , Pratama Rizqi Putra , Utami Tri Bekti , Fajarwati Alia , Hadiwijoyo Suryo Sakti
Published: April 2023

Abstrak Stunting merupakan salah satu fokus pembangunan di Kota Salatiga sebagai salah satu upaya untuk mewujudkan insan yang sehat dan cerdas menuju Salatiga yang bersih dan sehat. Tujuan dari studi ini adalah untuk mengidentifikasi kondisi stunting, menjelaskan faktor penyebab stunting, serta menganalisis strategi dan implementasi penanggulangan kasus stunting di Kota Salatiga melalui studi literatur, observasi lapangan, indepth interview dengan stakeholders, serta pengisian angket oleh keluarga terdampak stunting. Pengolahan data dilakukan dengan menggunakan analisis deskriptif kualitatif dan deskriptif kuantitatif serta dilakukan triangulasi menggunakan data-data sekunder yang telah dikumpulkan. Hasil kajian menunjukkan bahwa stunting disebabkan oleh berbagai faktor, seperti faktor kesehatan, faktor sosial budaya, dan faktor ekonomi yang tidak terlalu dominan. Isu stunting yang disebabkan oleh faktor dari berbagai dimensi sebisa mungkin diselesaikan dengan koordinasi berbagai pihak terkait, seperti Badan Perencanaan Pembangunan Daerah, Dinas Pemberdayaan Perempuan, Perlindungan anak, Pengendalian Penduduk dan Keluarga Berencana (DP3APP-KB), Kantor Urusan Agama, Puskesmas, dan tentu saja dengan dukungan masyarakat. Abstract Stunting is a development focus in Salatiga City to achieve healthy and intelligent human for the clean and health of Salatiga.. Aim of this study is to identify the condition of stunting, explain the cause, and analyze the strategy and implementation in Salatiga city by using literature review, observation, in-depth interviews with all stakeholders and also filling a questionnaire for the family who have stunting kids. Data processing was carried out using descriptive qualitative and quantitative analysis and its triangulated by secondary data that had been collected. Results found that stunting is caused by many factors like health conditions, culture and society, and economy but not dominant. Therefore, stunting is caused by many factors should be done by coordination and teamwork of all stakeholders: Development Planning Agency at Sub-National Level, Department of Women's Empowerment, Child Protection, Population Control, and Family Planning (DP3APP-KB), Department of Religious Affairs, public health center, and the most is support by citizens.
Karakteristik Tingkat Kematangan Buah Kopi Robusta (Coffea canephora A. Froehner) dan Buah Kopi Arabika (Coffea arabica Linnaelus) Terhadap Mutu dan Cita Rasa Seduhan Kopi
Authors: Alam Indra , Warkoyo Warkoyo , Siskawardani Devi Dwi
Published: April 2023

Salah satu komoditas yang paling menonjol dibidang perkebunan yaitu kopi. Faktor tingkat setiap kematangan buah kopi memiliki sifat dan karakteristik yang berbeda sehingga sangat mempengaruhi kualitas dan rasa dari hasil pengolahan buah kopi menjadi bubuk dan mempengaruhi mutu produk yang dihasilkan. Tujuan dari penelitian ini untuk mengetahui pengaruh antara tingkat kematangan buah kopi robusta dan arabika terhadap mutu dan cita rasa seduhan kopi, mengetahui nilai terbaik buah kopi robusta dan arabika terhadap mutu dan cita rasa seduhan kopi. Penelitian terdiri atas 2 tahap. Tahap pertama penentuan sifat buah sampai bubuk kopi, sedangkan tahap kedua penyeduhan kopi. Rancangan penelitian yang digunakan adalah Rancangan Acak Kelompok (RAK) Sederhana dengan dua faktor yaitu faktor I perbedaan varietas kopi dan faktor II adalah jenis tingkat kematangan buah kopi dan terdiri dari tiga ulangan. Hasil penelitian menunjukan bahwa tingkat kematangan kopi robusta dan arabika berpengaruh nyata terhadap sifat fisik kerapatan massa buah dan biji kopi, perbedaan ukuran kebulatan (sphericity) buah dan biji kopi, kadar air, pH, kecerahan, rendemen, dan berat endapan seduhan kopi serta hedonik aroma, rasa, keasaman, dan after taste. Nilai terbaik pada parameter kadar air adalah kopi robusta dengan tingkat kematangan merah (ripe), pH seduhan dan rendemen bubuk kopi adalah kopi arabika dengan tingkat kematangan merah (ripe), berat endapan seduhan adalah kopi robusta dengan tingkat kematangan merah (ripe), nilai terbaik hedonik aroma, after taste, dan kepekatan adalah kopi arabika dengan tingkat kematangan merah (ripe) sedangkan keasaman dan rasa adalah kopi robusta dengan tingkat kematangan merah (ripe).
La percepción de la situación pandémica por parte de la población española
Authors: Docampo García Manuel
Published: April 2023

Los objetivos que guían este texto son cuatro: 1) Reconstruir el marco teórico de la sociología de las actitudes; 2) Identificar las actitudes relevantes que la ciudadanía española mantenía ante la pandemia; 3) Medir el peso o porcentaje de población en cada una de ellas y, finalmente, 4) Caracterizar el perfil sociológico de cada tipo actitudinal. A estos, añadimos una reflexión u objetivo complementario, referido a la validez y utilidad de estas categorías, más allá del contexto de la pandemia de covid-19 entre la población española.
Harmony between the autonomy in private law and controls in public law: Focusing on provisional contract for real estate sales contract
Authors:   Wonkwang University Legal Research Institute has, Yang Mi-Suk , Park Shin-Uk
Published: April 2023

In principle, everyone is guaranteed freedom of contract. In principle, the contents of a real estate sales contract can also be freely determined by agreement between the seller and the buyer without state control. However, even if the contract is based on the civil law to which private autonomy is applied, there are some cases which could be also a subject to public law. For example, in accordance with ACT on report on real estate transaction (ARRET), the sales price of a real estate sales contract must be reported to the authority within 30 days from the date of conclusion of the sales contract. This is a kind of reporting obligation. Recently, it is common to conclude a provisional contract before concluding a real estate sales contract in Korea. Regarding this situation, namely provisional contract, there is a controversial case, in which a licensed real estate agent was fined for violating the reporting obligation. This is understood to be due to differences in perception of how to understand provisional contracts. In the controversial case, the authority judged the starting point of transaction in a report as the date of deposit of the provisional contract, not the date of conclusion of the main contract. The ARRET does not define the date of conclusion of a sales contract or provides a guideline for interpretation. The date of conclusion of the contract means the date on which the contract is actually concluded, not the date of written contract or the date on which the contract is drafted. Based on this date, the report must be made within the period. Regarding the date, there are several problems in the disposition of fine by the authority. First, fine for negligence are imposed for delay of reporting obligation, and the amount of fine actually imposed is due to false reports. Second, various contracts called provisional contracts cannot be identified as the same contract as the main contract. Third, there are cases in which reporting is impossible at the time an agreement on the sales contract is established. Imposing a fine for negligence is a kind of an intrusive administrative disposition that imposes certain obligations. Therefore, the legal basis for disposition must be clear, and extended or inferential interpretations are not permitted. Furthermore, the authority should judge more carefully whether the fundamental rights of the constitution are not violated or whether the legal basis for the disposition is clear when taking an intrusive administrative disposition against a private person.