bluuebunny/crossref_metadata_2025 huggingface dataset:
The Anthrax Toxin Receptor 1 (ANTXR1) Is Enriched in Pancreatic Cancer Stem Cells Derived from Primary Tumor Cultures
Authors: Alcalá Sonia , Martinelli Paola , Hermann Patrick C. , Heeschen Christopher , Sainz Bruno
Published: May 2019

Pancreatic ductal adenocarcinoma (PDAC) is currently the fourth leading cause of cancer-related mortality. Cancer stem cells (CSCs) have been shown to be the drivers of pancreatic tumor growth, metastasis, and chemoresistance, but our understanding of these cells is still limited by our inability to efficiently identify and isolate them. While a number of markers capable of identifying pancreatic CSCs (PaCSCs) have been discovered since 2007, there is no doubt that more markers are still needed. The anthrax toxin receptor 1 (ANTXR1) was identified as a functional biomarker of triple-negative breast CSCs, and PDAC patients stratified based on ANTXR1 expression levels showed increased mortality and enrichment of pathways known to be necessary for CSC biology, including TGF-β, NOTCH, Wnt/β-catenin, and IL-6/JAK/STAT3 signaling and epithelial to mesenchymal transition, suggesting that ANTXR1 may represent a putative PaCSC marker. In this study, we show that ANTXR1+ cells are not only detectable across a panel of 7 PDAC patient-derived xenograft primary cultures but ANTXR1 expression significantly increased in CSC-enriched 3D sphere cultures. Importantly, ANTXR1+ cells also coexpressed other known PaCSC markers such as CD44, CD133, and autofluorescence, and ANTXR1+ cells displayed enhanced CSC functional and molecular properties, including increased self-renewal and expression of pluripotency-associated genes, compared to ANTXR1- cells. Thus, this study validates ANTXR1 as a new PaCSC marker and we propose its use in identifying CSCs in this tumor type and its exploitation in the development of CSC-targeted therapies for PDAC.
Transcriptome Sequencing Reveals Regulatory Mechanisms of Taxol Synthesis in Taxus wallichiana var. Mairei
Authors: Wang Tao , Chen Yiming , Zhuang Weibing , Zhang Fengjiao , Shu Xiaochun , Wang Zhong , Yang Qing
Published: May 2019

Taxol is one of the most potent and effective anticancer drugs and is originally isolated from Taxus species. To investigate the specific regulatory mechanisms of taxol synthesis in Taxus wallichiana var. mairei, RNA-seq was conducted to reveal the differences in transcriptional levels between wild type (WT) and “Jinxishan” (JXS), a cultivar selected from a population of Taxus mairei that shows about 3-fold higher taxol content in the needles than WT. Our results indicated that high expressions of the genes taxadienol acetyltransferase (TAT), taxadiene 5-alpha hydroxylase (T5H), 5-alpha-taxadienol-10-beta-hydroxylase (T10OH), and 2-debenzoyl-7,13-diacetylbaccatin III-2-O-benzoyl-transferase (DBBT), which catalyze a series of key acetylation and hydroxylation steps, are the main cause of high taxol content in JXS. Moreover, in the present study, the activation of jasmonic acid (JA) signal transduction and its crosstalk with gibberellin (GA), auxin, and ethylene (ET) explained the elevation of differentially expressed genes (DEGs) from the taxol biosynthesis pathway. This also indicates that taxol biosynthesis in T. mairei is associated with the balance of cell development and defense. TF-encoding (transcriptional factor) genes, represented by the ethylene-responsive transcription factor (ERF), basic/helix-loop-helix (bHLH), MYB, and WRKY families, were detected as differentially expressed between JXS and WT, further indicating that the regulation of hormone signaling on taxol biosynthesis genes was mediated by transcription factors (TFs). To our knowledge, this is the first study to illustrate the regulatory mechanisms of taxol synthesis in a new cultivar of T. mairei with a high taxol content in its needles. These transcriptome data provide reasonable explanations for the variation of taxol content between WT and JXS.
MicroRNAs: Game Changers in the Regulation ofα-Synuclein in Parkinson’s Disease
Authors: Zhao Liang , Wang Zhiqin
Published: May 2019

Parkinson’s disease (PD) is the second most common neurodegenerative disorder. Its neuropathological hallmarks include neuronal loss in the substantia nigra pars compacta (SNpc) and the presence of Lewy bodies containing aggregates ofα-synuclein (α-syn). An imbalance between the rates ofα-syn synthesis, aggregation, and clearance can result in abnormalα-syn levels and contribute to the pathogenesis of PD. MicroRNAs (miRNAs) are endogenous single-stranded noncoding RNAs (∼22 nucleotides) that have recently emerged as key posttranscriptional regulators of gene expression. In this review, we summarize the functions of miRNAs that directly targetα-syn. We also review miRNAs that indirectly impactα-syn levels or toxicity through different pathways, including those involved in the clearance ofα-syn and neuroinflammation.
A Robust Spline Collocation Method for Pricing American Put Options
Authors: Cen Zhongdi , Le Anbo , Xu Aimin
Published: May 2019

In this paper a robust numerical method is proposed for pricing American put options. The Black-Scholes differential operator in the original form is discretized by using a quadratic spline collocation method on a piecewise uniform mesh for the spatial discretization and the implicit Euler scheme for the time discretization. The position of collocation points is chosen so that the spline difference operator satisfies the discrete maximum principle, which guarantees that the scheme is maximum-norm stable. The error estimation is derived by applying the maximum principle to the discrete linear complementarity problem in two mesh sets. It is proved that the scheme is second-order convergent with respect to the spatial variable and first-order convergent with respect to the time variable. Numerical results demonstrate that the scheme is stable and accurate.
Potentiometric Determination of Chlorate Impurities in Hypochlorite Solutions
Authors: Girenko Dmitry V. , Gyrenko Al’ona A. , Nikolenko Nikolai V.
Published: May 2019

The method of iodometric determination of chlorates impurities in sodium hypochlorite solutions for medical and veterinary purposes was developed. This method does not require sophisticated equipment and can be implemented directly where the solutions are used. The method is based on the different rates of interaction of ClO- and ClO3- with iodide ions depending on the acidity of the medium. We have shown that blank titration is advisable to improve the accuracy of the determination of low concentrations of chlorates in the matrix of hypochlorite which is present in excess since in this case possible systematic errors due to the presence of oxidizing impurities in the reagents are prevented. To quantify the low concentrations of chlorates, we proposed to remove 85-95% of hypochlorite ions by means of reducing their excess with sodium sulfite at pH 10.5. The solution of sodium sulfite does not require standardization before each analysis in the proposed procedure. The possibility of quantitative determination of chlorate impurities in the range of 2-50 mg/L in the presence of 50-500–fold excess of sodium hypochlorite with an error of 5% has been proved. The expanded uncertainty of chlorate determination did not exceed 0.6 mg/L.
A Validated HPLC-MS/MS Method for Simultaneous Determination of Militarine and Its Three Metabolites in Rat Plasma: Application to a Pharmacokinetic Study
Authors: Sun Hui-Yuan , Zheng Lin , Gong Zi-Peng , Li Yue-Ting , Yang Chang , Pan Jie , Wang Yong-Lin , Wang Ai-Min , Li Yong-Jun , Huang Yong
Published: May 2019

A rapid, reliable, and sensitive HPLC-electrospray ionization-tandem mass spectrometry (HPLC-MS/MS) method was established and validated for simultaneous determination of militarine and its three metabolites (gastrodin, α-isobutylmalic acid, and gymnoside I) in rat plasma. Plasma was acidified with formic acid, and protein was precipitated with methanol. MS/MS with ESI and multiple reaction monitoring at m/z 725.3→457.3, 457.1→127, 304.3→107.2, 189→129, and 417.1→267.1 was used for determination of militarine, gastrodin, α-isobutylmalic acid, gymnoside I, and puerarin (internal standard), respectively. Chromatographic separation was conducted using an ACE UltraCore SuperC18 (2.1 × 100 mm, 2.5 μm) column with gradient mobile phase (0.1% formic acid in water and acetonitrile). The lower limits of quantitation for militarine, gastrodin, α-isobutylmalic acid, and gymnoside I were 1.02, 2.96, 1.64, and 0.3 ng/mL, respectively. The relative standard deviations of intra- and interday measurements were less than 15%, and the method accuracy ranged from 87.4% to 112.5%. The extraction recovery was 83.52%-105.34%, and no matrix effect was observed. The three metabolites (gastrodin, α-isobutylmalic acid, and gymnoside I) were synchronously detected at 0.83 h, suggesting that militarine was rapidly transformed to gastrodin, α-isobutylmalic acid, and gymnoside I. Moreover, the area under the curve (AUC) and Cmax of militarine were significantly lower than those of gastrodin and α-isobutylmalic acid, showing that militarine was largely metabolized to gastrodin and α-isobutylmalic acid in vivo. The studies on pharmacokinetics of militarine and its three metabolites were of great use for facilitating the clinical application of militarine and were also highly meaningful for the potential development of militarine.
Cell Morphology on Poly(methyl methacrylate) Microstructures as Function of Surface Energy
Authors: Katschnig Matthias , Maroh Boris , Andraschek Natascha , Schlögl Sandra , Zefferer Ulrike , Bock Elisabeth , Leitinger Gerd , Trattnig Christa , Kaufmann Maria , Balika Werner , Holzer Clemens , Schäfer Ute , Patz Silke
Published: May 2019

Whilst the significance of substrate topography as a regulator of cell function is well established, a systematic analysis of the principles underlying this is still unavailable. Here we evaluate the hypothesis that surface energy plays a decisive role in substrate-mediated modulation of cell phenotype by evaluation of cell behaviour on synthetic microstructures exhibiting pronounced differences in surface energy. These microstructures, specifically cubes and walls, were fabricated from a biocompatible base polymer, poly(methyl methacrylate), by variotherm injection molding. The dimensions of the cubes were 1 μm x 1 μm x 1 μm (height x width x length) with a periodicity of 1:1 and 1:5 and the dimensions of the walls 1 μm x 1 μm x 15 mm (height x width x length) with a periodicity of 1:1 and 1:5. Mold inserts were made by lithography and electroplating. The surface energy of the resultant microstructures was determined by static contact angle measurements. Light scanning microscopy of the morphology of NT2/D1 and MC3T3-E1 preosteoblast cells cultured on structured PMMA samples in both cases revealed a profound surface energy dependence. “Walls” appeared to promote significant cell elongation, whilst a lack of cell adhesion was observed on “cubes” with the lowest periodicity. Contact angle measurements on walls revealed enhanced surface energy anisotropy (55 mN/m max., 10 mN/m min.) causing a lengthwise spreading of the test liquid droplet, similar to cell elongation. Surface energy measurements for cubes revealed increased isotropic hydrophobicity (87° max., H2O). A critical water contact angle of ≤ 80° appears to be necessary for adequate cell adhesion. A “switch” for cell adhesion and subsequently cell growth could therefore be applied by, for example, adjusting the periodicity of hydrophobic structures. In summary cell elongation on walls and a critical surface energy level for cell adhesion could be produced for NT2/D1 and MC3T3-E1 cells by symmetrical and asymmetrical energy barrier levels. We, furthermore, propose a water-drop model providing a common physicochemical cause regarding similar cell/droplet geometries and cell adhesion on the investigated microstructures.
Induced Ripening Agents and Their Effect on Fruit Quality of Banana
Authors: Maduwanthi S. D. T. , Marapana R. A. U. J.
Published: May 2019

Ripening is a genetically programmed highly coordinated irreversible phenomenon which includes many biochemical changes including tissue softening, pigment changes, aroma and flavour volatile production, reduction in astringency, and many others. Banana is one of mostly consumed fruit crops in the world. Since banana is a climactic fruit, induced ripening is essential in commercial scale banana cultivation and distribution to assure good flavour, texture, and uniform peel colour. Ethylene gas, acetylene gas liberated from calcium carbide, and ethephon are some of the commercial ripening agents used successfully in the trade and they have been widely studied for their effectiveness on initiating and accelerating the ripening process and their effect on fruit quality and health related issues. Lauryl alcohol was also shown as a ripening agent for bananas. Most studies suggest that there is no difference in biochemical composition and sensory quality in bananas treated with chemicals that induce ripening from naturally ripened bananas. However volatile profiles of artificially ripened bananas were shown to be considerably different from naturally ripened bananas in some studies. This review discusses induced ripening agents and their effect on fruit quality of bananas.
The Effects of Pitavastatin on Nuclear Factor-Kappa B and ICAM-1 in Human Saphenous Vein Graft Endothelial Culture
Authors: Demir Bulent , Onal Burak , Ozyazgan Sibel , Demir Esra , Bakuy Vedat , Akkan Ahmet Gokhan
Published: May 2019

Objective. To study pitavastatin’s effects on nuclear factor-kappa B (NF-κB ) and adhesion molecules in human saphenous vein graft endothelial culture indicating its pleotropic properties. Materials and Method. Low-dose (0.1 μM/L) and high-dose (1μM/L) pitavastatin calcium were administered as a frontline therapy in human saphenous endothelial cell culture, followed by induction of inflammation by TNF-α and determination of mRNA level alterations of ICAM-1 and NF-κB genes of endothelial cells using the qRT-PCR method. Additionally, immunofluorescence method was used to show the expression of NF-κB and ICAM-1. Finally, LDH levels were determined by the ELISA method to quantify cytotoxicity. Results. ICAM-1 mRNA expression in the low-dose pitavastatin+TNF-α group was significantly higher than that in the TNF-α group and significantly lower than that in the high-dose pitavastatin+TNF-α group (for all comparisons, P = 0.001). The low-dose pitavastatin+TNF-α group had a similar NF-κB mRNA expression with TNF-α and high-dose pitavastatin+TNF-α groups. Conclusion. Pitavastatin increases ICAM-1 mRNA expression in saphenous vein endothelial cells. Furthermore, the effect of pitavastatin on adhesion molecules appears independent of NF-κB. Novel studies are needed in this field.
Distributed Structured Compressive Sensing-Based Time-Frequency Joint Channel Estimation for Massive MIMO-OFDM Systems
Authors: Zhang Wenjie , Li Hui , Jin Rong , Wei Shanlin , Cheng Wei , Kong Weisi , Liu Penglu
Published: May 2019

In massive multi-input multi-output orthogonal frequency division multiplexing (MIMO-OFDM) systems, accurate channel state information (CSI) is essential to realize system performance gains such as high spectrum and energy efficiency. However, high-dimensional CSI acquisition requires prohibitively high pilot overhead, which leads to a significant reduction in spectrum efficiency and energy efficiency. In this paper, we propose a more efficient time-frequency joint channel estimation scheme for massive MIMO-OFDM systems to resolve those problems. First, partial channel common support (PCCS) is obtained by using time-domain training. Second, utilizing the spatiotemporal common sparse property of the MIMO channels and the obtained PCCS information, we propose the priori-information aided distributed structured sparsity adaptive matching pursuit (PA-DS-SAMP) algorithm to achieve accurate channel estimation in frequency domain. Third, through performance analysis of the proposed algorithm, two signal power reference thresholds are given, which can ensure that the signal can be recovered accurately under power-limited noise and accurately recovered according to probability under Gaussian noise. Finally, pilot design, computational complexity, spectrum efficiency, and energy efficiency are discussed as well. Simulation results show that the proposed method achieves higher channel estimation accuracy while requiring lower pilot sequence overhead compared with other methods.
Biosynthesis and Characterization of Iron Nanoparticles for Effective Adsorption of Cr(VI)
Authors: Önal Emel Simla , Yatkın Tolga , Aslanov Tural , Ergüt Memduha , Özer Ayla
Published: May 2019

In this study, iron nanoparticles (FeNPs) were synthesized via a green method using loquat (Eriobotrya japonica) leaves aqueous extract as a renewable reducing agent. The synthesized FeNPs were characterized by DLS, XRD, FT-IR, SEM/EDX, and TEM analysis, and then, they were used as an adsorbent for Cr(VI) removal from aqueous solutions. Batch adsorption experiments were carried out to investigate the optimum adsorption parameters such as the initial pH of the solution, temperature, initial Cr(VI) concentration, and adsorbent concentration. The optimum adsorption conditions were determined as initial pH 3.0, temperature 45°C, and adsorbent concentration 1 g/L. Also, a linear increase was observed in adsorbed Cr(VI) amounts with the increasing initial Cr(VI) concentrations. The biosynthesized FeNPs showed the high removal levels higher than 90% for Cr(VI) adsorption at a wide range of initial Cr(VI) concentrations (50–500 mg/L). The experimental equilibrium data were modelled with Langmuir and Freundlich isotherm models, and it was found that experimental equilibrium data could be well described by the Langmuir isotherm model. The maximum monolayer coverage capacity of FeNPs for Cr(VI) adsorption was found to be 312.5 mg/g. The pseudo-first-order and the pseudo-second-order kinetic models were applied to the experimental adsorption data, and it was concluded that the data were defined as the best agreement with the pseudo-second-order kinetic model. Weber–Morris model was used to investigate the effect of mass transfer on the adsorption of Cr(VI) onto FeNPs; it was observed that both the film (boundary layer) and intraparticle diffusion affected the studied adsorption process. The thermodynamic studies suggested that Cr(VI) adsorption onto FeNPs was endothermic and nonspontaneous, and the positive ΔS value indicated increased disorder at the solid-solution interface during the adsorption.
Association between Central Serous Chorioretinopathy and Risk of Depression: A Population-Based Cohort Study
Authors: Chen Yu-Yen , Huang Li-Ying , Liao Wei-Ling , Chou Pesus
Published: May 2019

Purpose. To investigate the association between central serous chorioretinopathy (CSC) and the risk of developing depression. The risk factors associated with depression in CSC patients were also assessed. Methods. A population-based retrospective cohort study using the Taiwan National Health Insurance Research Database was conducted from the beginning of 2001 through the end of 2013. CSC patients and age- and gender-matched (1 : 4 matched) control subjects without CSC were enrolled in the study. Kaplan–Meier curves were generated to compare the cumulative hazard of subsequent depression between the CSC and control groups. A Cox regression analysis estimated the crude and adjusted hazard ratios (HRs) for depression. Risk factors leading to depression were investigated among the CSC patients. Results. 25,939 CSC patients and 103,756 controls were enrolled in the study. The CSC group had a significantly higher cumulative hazard for depression compared to the control group (p value < 0.0001). The Cox regression model indicated that the CSC group had a significantly higher risk for depression (adjusted HR = 1.33). Within the CSC group, significant risk factors for depression included age, female gender, low income, first-onset CSC, peptic ulcer, and smoking. The recent use of steroids prior to CSC, by all routes of administration, also significantly increased the risk for depression. However, treatment of CSC did not significantly reduce the risk for depression. Conclusion. Patients with CSC are at significantly greater risk of developing depression. Among CSC patients, age, female gender, low income, first-onset CSC, peptic ulcer, smoking, and recent use of steroids prior to CSC were significant risk factors for depression.
Application and Analysis of an Ionic Liquid Gel in a Soft Robot
Authors: Zhang Chenghong , He Bin , Wang Zhipeng , Zhou Yanmin , Ming Aiguo
Published: May 2019

Due to their light weight, flexibility, and low energy consumption, ionic electroactive polymers have become a hotspot for bionic soft robotics and are ideal materials for the preparation of soft actuators. Because the traditional ionic electroactive polymers, such as ionic polymer-metal composites (IPMCs), contain water ions, a soft actuator does not work properly upon the evaporation of water ions. An ionic liquid polymer gel is a new type of ionic electroactive polymer that does not contain water ions, and ionic liquids are more thermally and electrochemically stable than water. These liquids, with a low melting point and a high ionic conductivity, can be used in ionic electroactive polymer soft actuators. An ionic liquid gel (ILG), a new type of soft actuator material, was obtained by mixing 1-butyl-3-methylimidazolium tetrafluoroborate (BMIMBF4), hydroxyethyl methacrylate (HEMA), diethoxyacetophenone (DEAP) and ZrO2 and then polymerizing this mixture into a gel state under ultraviolet (UV) light irradiation. An ILG soft actuator was designed, the material preparation principle was expounded, and the design method of the soft robot mechanism was discussed. Based on nonlinear finite element theory, the deformation mechanism of the ILG actuator was deeply analyzed and the deformation of the soft robot when grabbing an object was also analyzed. A soft robot was designed with the soft actuator as the basic module. The experimental results show that the ILG soft robot has good driving performance, and the soft robot can grab a 105 mg object at an input voltage of 3.5 V.
Subparalyzing Doses of Rocuronium Reduce Muscular Endurance without Detectable Effect on Single Twitch Height in Awake Subjects
Authors: Gelberg Jan , Bentzer Peter , Grubb David
Published: May 2019

Purpose. To test the hypothesis that a low-dose rocuronium acts mainly by means of reducing muscular endurance rather than by reducing momentary force. Methods. In a randomized placebo-controlled double-blinded study, eight healthy volunteers were studied in two sets of experiments. In the first set, the subjects made a sustained maximum effort with the dominant hand for 80 seconds while squeezing an electronic handgrip dynamometer at three minutes after intravenous administration of placebo, 0.04 or 0.08 mg/kg rocuronium. Handgrip force at initiation of testing (maximum handgrip force) and after 60 seconds was evaluated. In the second set, the ulnar nerve of the subjects was electrically stimulated every tenth second for at least 10 and a maximum of 30 minutes following the administration of placebo and 0.08 mg/kg rocuronium. Single twitch height of the adductor pollicis muscle was recorded. Results. There was no significant difference in the effect on maximum handgrip force at time 0 between the three different doses of rocuronium. As compared with placebo, handgrip force after 0.08 mg/kg rocuronium was reduced to approximately a third at 60 seconds (214 N (120–278) vs. 69 (30–166); p=0.008), whereas only a slight reduction was seen after 0.04 mg/kg (187 (124–256); p=0.016). Based on these results, the sustained handgrip force after 0.2 mg/kg at 60 seconds was calculated to be 1.27% (95% CI [0.40, 4.03]) of the maximum force of placebo. No effect on single twitch height after 0.08 mg/kg rocuronium at four minutes after drug administration could be detected. Conclusions. Subparalyzing doses of rocuronium show a distinct effect on muscular endurance as opposed to momentary force. The findings support the hypothesis that low doses of rocuronium act mainly by reducing muscular endurance, thereby facilitating, for example, tracheal intubation.
Chitosan-Based Polyion Multilayer Coating on NPK Fertilizer as Controlled Released Fertilizer
Authors: Kusumastuti Yuni , Istiani Alit , Rochmadi  , Purnomo Chandra Wahyu
Published: May 2019

Low efficiency of conventional fertilizer (quick release fertilizer) application in agricultural sectors has caused environmental pollution and health problems. A method to overcome the drawback of the conventional fertilizer is by controlled release fertilizer (CRF) preparation. CRF is expected to be able to fulfil the nutrient demand of targeted plants. The objective of this research is to prepare CRF by coating NPK fertilizer with multilayer chitosan-polyanion using alginate, pectin, and sodium tripolyphosphate (TPP). In addition, the effect of the layer arrangement modification of material on the rate of nitrogen release was also studied. The mechanical strength of coated fertilizer was analysed by compressive stress test and the properties of the fertilizer coating was observed by scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR). The nitrogen release study shows that multilayer of chitosan-alginate (CA)5, chitosan-pectin (CP)5, and chitosan-TPP (CT)5 as coating material was able to increase the compressive stress and decrease the nitrogen release of coated fertilizer. These results are supported by the FTIR analysis which exhibits the formation of ionic interaction between amine group of chitosan and carboxyl group of alginate in chitosan-alginate (CA)5 layer, carboxyl group of pectin in chitosan-pectin (CP)5 layer, and phosphate of TPP in chitosan-TPP (CT)5 layer. On the other hand, the modification of the arrangement of chitosan-alginate layers showed that the fertilizer with the alternating layer arrangement (CA)5 was able to optimally increase the compressive strength. The mathematical model for the nitrogen release of coated fertilizer is also prepared and simulated with the MATLAB software. The simulation results showed that the nitrogen release of coated fertilizer followed the proposed diffusion mechanism. The obtained diffusivity coefficient value in the layer of chitosan-alginate (CA)5 is 2.0933 × 10−6 cm2/s, 2.5606 × 10−6 cm2/s in chitosan-TPP (CT)5 layer, and 3.7256 × 10−6 cm2/s in chitosan-pectin (CP)5 layer.
Effects of Breed, Age, Body Condition Score, and Nutritional Status on Follicular Population, Oocyte Yield, and Quality in Three Cameroonian Zebus CattleBos indicus
Authors: Dorice Azafack Kana , Ferdinand Ngoula , Justin Kouamo , Augustave Kenfack , Linda Kenne Kaze
Published: May 2019

This study was aimed at investigating the effects of breed, age, body condition score, and nutritional status on follicular dynamics, quality, and oocyte yield in Cameroonian zebus cattleBos indicus(Gudali and Red and White Fulani). A total of 95 cows were investigated for breed, age, body condition score, and nutritional status. Blood samples and ovaries of these cows were collected during slaughtering. Follicles on each ovary were counted and measured using electronic digital calipers and then classified into three categories: small (<3 mm), medium (3 – 8 mm), and large (> 8 mm) diameter. The oocytes were retrieved by slicing each ovary into a Petri dish containing collected medium. The oocytes recovered were examined under a stereoscope (x 10) and classified into four groups based on the morphology of cumulus oophorus cells and cytoplasmic changes. From these ovaries, 3888 follicles were counted with an average population of 27.81 ± 6.88 follicles per cow. The average numbers of small, medium, and large follicles per cow were 17.26 ± 1.36 (62.06%), 9.64 ± 1.00 (34.66%), and 0.52 ± 0.32 (1.86%), respectively. A total of 1822 oocytes were harvested with an average of 19.11 ± 0.89 oocytes per cow. Oocytes graded I, II, III, and IV were 7.03 ± 2.30 (36.65%), 4.53± 2.01 (23.61%), 3.19 ± 0.85 (16.63%), and 4.43 ±1.62 (23.09%), respectively. This study also showed that ovarian weight, number of follicles, and oocytes’ yield of right ovary were higher than left ones (P < 0.05). Younger and normal cows (BCS = 3) presented higher (P < 0.05) number of follicles and oocytes. Urea and cholesterol levels were higher in Gudali and White Fulani cows (P < 0.05). This study indicated that ovaries of zebus harvested in slaughterhouse are an important source of follicular population, oocytes yield, and quality. Right ovaries are heavy and offer more numbers of follicles and oocytes than the left ones. BCS is the best indicator of nutritional status of cows. Important follicle populations are obtained from cows aging 6-9 years with BCS = 3.
Visceral Fat Accumulation Is Associated with Asthma in Patients with Type 2 Diabetes
Authors: Murakami Daisuke , Anan Futoshi , Masaki Takayuki , Umeno Yoshikazu , Shigenaga Takehiko , Eshima Nobuoki , Nakagawa Takashi
Published: May 2019

Objective. The number of patients with type 2 diabetes has increased in Japan, and type 2 diabetes has attracted attention as a risk factor for asthma. However, the risk factors for the development of asthma in patients with type 2 diabetes have not been determined. This study was performed to clarify whether visceral fat accumulation (VFA) and insulin resistance are risk factors for the development of asthma in patients with type 2 diabetes. Materials and Methods. A cross-sectional study was conducted. The study group comprised 15 patients with type 2 diabetes with asthma, and the control group comprised 145 patients with type 2 diabetes without asthma. Their fat distribution was evaluated by measuring the VFA by abdominal computed tomography at the umbilical level. Their glucose status was assessed by measuring the fasting plasma glucose (FPG) concentration, fasting immunoreactive insulin concentration, homeostasis model assessment (HOMA) index, and hemoglobin A1c concentration. Results. Among patients with type 2 diabetes, VFA was significantly greater in patients with asthma than those without asthma (P<0.0001). The FPG concentration, fasting immunoreactive insulin concentration, and HOMA index were higher in patients with asthma than those without asthma (P<0.05, P<0.0001, and P<0.0001, respectively). Multiple logistic regression analysis showed that VFA and the HOMA index were significantly associated with asthma in patients with type 2 diabetes (odds ratio, 1.78; 95% confidence interval, 1.31–3.89; P=0.0115 and odds ratio, 3.65; 95% confidence interval, 1.37–7.85; P=0.0078, respectively). Conclusions. Our data suggest that VFA and insulin resistance are associated with the development of asthma in patients with type 2 diabetes.
Study of the Fracture Law of Overlying Strata under Water Based on the Flow-Stress-Damage Model
Authors: Wen Zhijie , Jing Suolin , Jiang Yujing , Tian Lei , Wen Jinhao , Cao Zhiguo , Shi Shaoshuai , Zuo Yujun
Published: May 2019

To accurately detect the development height of the water flowing fractured zone (WFFZ) in the overlying strata of the working face after mining under water and to ensure the safety and reliability of coal mining, the coal seam located under Weishanhu Lake in the Jisan coal mine was used as the experimental system. A similar laboratory simulation and water injection-based fracturing test system were used with the working face before and after mining activity to calculate, quantitatively detect, and qualitatively analyze the development height of the WFFZ in the overlying strata. Meanwhile, a flow-stress-damage model and its criterion of fracture expansion were established based on the Mohr-Coulomb criterion, and the FLAC 3D software was used to simulate the deformation and failure of the overlying strata and the evolution of WFFZ during the mining process. The results showed that the height ranges of the WFFZ beneath Weishanhu Lake of the Jisan coal mine as established by the above three methods are 30-45 m, 30-48 m, and 30-50 m. In the process of mining, the caving zone and fractured zone are, respectively, subjected to tensile failure and shear failure. The development height of the water flowing through the fractured zone in the overlying strata is basically consistent with the range of the “breaking arch.” The flow-stress-damage model and its criterion of fracture expansion can be applied to the fracture law of overlying strata under water under similar geological conditions.
Hypertension in Jordan: Prevalence, Awareness, Control, and Its Associated Factors
Authors: Khader Yousef , Batieha Anwar , Jaddou Hashem , Rawashdeh Sukaina I. , El-Khateeb Mohammed , Hyassat Dana , Khader Albaraa , Ajlouni Kamel
Published: May 2019

Objectives. Determine the prevalence, awareness, and control rates of hypertension and their associated factors among Jordanian adults. Methods. A multistage sampling technique was used to select a nationally representative sample of adults from the population of Jordan. Trained interviewers collected data using a comprehensive structured questionnaire, measured anthropometric parameters, and collected blood samples. Results. This study included a total of 1193 men and 2863 women aged ranged from 18 to 90 year with a mean (SD) of 43.8 (14.2) year. The age-standardized prevalence was 33.8% among men and 29.4% among women. Of those with hypertnsion, 57.7% of men and 62.5% of women were aware of hypertension. Only 30.7% of men and 35.1% of women who were on antihypertensive medications had their blood pressure controlled. From 2009 to 2017, there was nonsignificant decrease in hypertension prevalence of 2.7% among men and 1.1% among women. However, the rate of hypertension awareness increased significantly among men and among women. Discussion. Almost one-third of Jordanian adults had hypertension. Interventions that target modifiable risk factors of hypertension, might decrease blood pressure, and even prevent the development of hypertension should be implemnted.
Service Life Prediction of Precast Concrete Structures Exposed to Chloride Environment
Authors: Zhang Dawei , Zeng Yu , Fang Mingshan , Jin Weiliang
Published: May 2019

Chloride‐induced corrosion is widely accepted as one of the primary causes of premature deterioration for concrete structures in marine or deicing salt environment. For precast concrete (PC) structures, such durability problems may even be severer because defects in joint areas, e.g., cracks caused by grout shrinkage and improper construction, can accelerate chloride ion transportation process and may cause the interface shear failure when subjected to seismic load. By applying the path probability model (PPM) and reliability theory, a probabilistic framework was proposed to predict three limit states of PC structures, including corrosion initiation, serviceability limit state, and ultimate limit state. Using Monte Carlo simulation, a beam‐to‐column joint was further analyzed to illustrate the differences between PC structures and those cast in situ. The analysis indicates that corrosion initiation and serviceability limit state are sensitive to chloride diffusivity at connection area, and the higher pitting factor can significantly influence the bearing capacities of PC structures.
Thermomechanical Optimization and Comparison of a Low Thermal Inertia Mold with Rectangular Heating Channels and a Conventional Mold
Authors: Collomb Jean , Balland Pascale , Francescato Pascal , Gardet Yves , Leh David , Saffré Philippe
Published: May 2019

Molds used to manufacture high-performance composites currently do not meet the demand of manufacturers in terms of production rate due to massive mold designs, using straight-through heating channels, that are not thermally reactive. In this paper, using a thermal finite element model, the thermomechanical responses of an existing massive and conventional mold is observed; then, thermomechanical optimizations are carried out on a circular heating channel mold and on a rectangular heating channel mold. The objective of this paper is two-fold: (i) confirm the need to change design rules for molds considering technological aspects (e.g., pressure drop and fluid nature) and (ii) validate the advantages of an innovative concept of a low thermal inertia mold with rectangular heating channels. Results of this study confirm the need to reduce the mass of structures to increase heating rates and the importance of taking into account technological data (heat transfer fluid, pressure drop) to ensure the optimal convective exchange. After optimization, a decrease greater than 75% in heating time for the circular channel model and up to 88% for the rectangular channel model was observed. Moreover, the antagonistic nature between heating rate and thermal homogeneity of the molding surface and between heating rate and mechanical strength is confirmed.
Altered CSF Proteomic Profiling of Paediatric Acute Lymphocytic Leukemia Patients with CNS Infiltration
Authors: Mo Fei , Ma Xuelei , Liu Xiaobei , Zhou Ruofan , Zhao Yunuo , Zhou Hui
Published: May 2019

Background. For childhood acute lymphocytic leukemia (ALL), central nervous system leukemia (CNSL) is still the main reason of treatment failure. Changes of cerebrospinal fluid (CSF) proteome are deemed to occur after intrathecal chemotherapy. Objective. To find critical CSF biomarkers, which could be utilized to increase diagnostic and prognostic accuracy of CNSL. Methods. We performed proteomic profiling of CSF before and after the treatment of six sporadic paediatric patients diagnosed as ALL with central nervous system (CNS) involvement. CSF samples were properly processed and analyzed through the use of label-free liquid chromatography-tandem mass spectrometry (LC-MS/MS). Results. Among identified 428 unique proteins in all CSF samples, we quantified 10 altered proteins with diverse biological functions after induction chemotherapy. Conclusions. The levels of those 10 proteins change during the treatment of CNSL. Some of the proteins are likely to play a vital biological role as biomarkers for the development of ALL. In addition, our results indicated the feasible and reproducible utility of CSF for diagnosis and prognosis of patients with CNSL.
Rockburst Prediction Model Based on Entropy Weight Integrated with Grey Relational BP Neural Network
Authors: Zheng Yuchao , Zhong Heng , Fang Yong , Zhang Wensheng , Liu Kai , Fang Jing
Published: May 2019

A rockburst prediction model of the entropy weight grey relational backpropagation (BP) neural network is developed. The model needs to select the evaluation factors according to the engineering practice and establish the sample library. The entropy weight method is used to calculate the objective weight of the characteristic factors, and the similarity between the samples is calculated by the combination of grey relational theory and the entropy method. The training sample of the BP neural network is selected by threshold determination. Finally, we use the trained neural network to estimate the rockburst intensity grade of samples to be tested. This model is applied to the rockburst prediction of Qamchiq tunnel project, and the prediction results are in good agreement with the actual conditions of the subsequent construction, thus verifying the feasibility and effectiveness of the model in the rockburst prediction.
A Truncation Method for Solving the Time-Fractional Benjamin-Ono Equation
Authors: Ali Mohamed R.
Published: May 2019

We deem the time-fractional Benjamin-Ono (BO) equation out of the Riemann–Liouville (RL) derivative by applying the Lie symmetry analysis (LSA). By first using prolongation theorem to investigate its similarity vectors and then using these generators to transform the time-fractional BO equation to a nonlinear ordinary differential equation (NLODE) of fractional order, we complete the solutions by utilizing the power series method (PSM).
Unquenching the Quark Model in a Nonperturbative Scheme
Authors: Ortega Pablo G. , Entem David R. , Fernández Francisco
Published: May 2019

In recent years, the discovery in quarkonium spectrum of several states not predicted by the naive quark model has awakened a lot of interest. A possible description of such states requires the enlargement of the quark model by introducing quark-antiquark pair creation or continuum coupling effects. The unquenching of the quark models is a way to take these new components into account. In the spirit of the Cornell Model, this is usually done by coupling perturbatively a quark-antiquark state with definite quantum numbers to the meson-meson channel with the closest threshold. In this work we present a method to coupled quark-antiquark states with meson-meson channels, taking into account effectively the nonperturbative coupling to all quark-antiquark states with the same quantum numbers. The method will be applied to the study of the X(3872) resonance and a comparison with the perturbative calculation will be performed.
High Voltage Ride through Control of PMSG-Based Wind Turbine Generation System Using Supercapacitor
Authors: Liu Guangchen , Hu Jianwen , Tian Guizhen , Xu Lie , Wang Shengtie
Published: May 2019

Regarding PMSG-based wind turbine generation system, this paper proposes a supercapacitor energy storage unit (SCESU) which is connected in parallel with the DC-link of the back-to-back converter to enhance its high voltage ride through performance. The analysis of the operation and control for the grid-side converter and SCESU are conducted. Based on real time digital simulators (RTDS), a model and a Hardware-in-the-Loop (HiL) platform of PMSG-based wind turbine with SCESU is developed, and the simulation results show that the SCESU absorbs the imbalanced energy and the grid-side converter absorbs inductive reactive power during the period of voltage swell and verify the correctness and feasibility of the high voltage ride through control strategy.
Motion Reliability Analysis of the Delta Parallel Robot considering Mechanism Errors
Authors: Li Yu , Shang Deyong , Fan Xun , Liu Yue
Published: May 2019

Delta parallel robots are widely used in assembly detection, packaging sorting, precision positioning, and other fields. With the widespread use of robots, people have increasing requirements for motion accuracy and reliability. This paper considers the influence of various mechanism errors on the motion accuracy and analyzes the motion reliability of the mechanism. Firstly, we establish a kinematic model of the robot and obtain the relationship between the position of the end effector and the structural parameters based on the improved D–H transform rule. Secondly, an error model considering the dimension error, the error of revolute joint clearance, driving error, and the error of spherical joint clearance is established. Finally, taking an actual robot as an example, the comprehensive influence of mechanism errors on motion accuracy and reliability in different directions is quantitatively analyzed. It is shown that the driving error is a key factor determining the motion accuracy and reliability. The influence of mechanism errors on motion reliability is different in different directions. The influence of mechanism errors on reliability is small in the vertical direction, while it is great in the horizontal direction. Therefore, we should strictly control the mechanism errors, especially the driving angle, to ensure the motion accuracy and reliability. This research has significance for error compensation, motion reliability analysis, and reliability prediction in robots, and the conclusions can be extended to similar mechanisms.
Investigating the Protein Signature of Adamantinomatous Craniopharyngioma Pediatric Brain Tumor Tissue: Towards the Comprehension of Its Aggressive Behavior
Authors: Martelli Claudia , Serra Riccardo , Inserra Ilaria , Rossetti Diana Valeria , Iavarone Federica , Vincenzoni Federica , Castagnola Massimo , Urbani Andrea , Tamburrini Gianpiero , Caldarelli Massimo , Massimi Luca , Desiderio Claudia
Published: May 2019

Although histologically benign, adamantinomatous craniopharyngioma (AC) pediatric brain tumor is a locally aggressive disease that frequently determines symptoms and hormonal dysfunctions related to the mass effect on the surrounding structures. Another typical feature of this benign neoplasm is the presence of voluminous liquid cysts frequently associated with the solid component. Even if studies have been devoted to the proteomic characterization of the tumor intracystic fluid, poor explorations have been performed on its solid part, principally investigated by transcriptomics technologies. In the present study, seven specimens of AC whole tumor tissue have been analyzed by LC-MS for a preliminary assessment of the proteomic profile by a top-down/bottom-up integrated approach. Thymosin beta 4, ubiquitin, calmodulin, S100 proteins, prothymosin α isoform 2, alpha-defensins 1-4, and fragments largely belonging to vimentin, hemoglobin, and glial fibrillary acidic protein characterized the intact proteome. The identification of alpha-defensins, formerly characterized in AC intracystic fluid, reinforces the hypothesis of a role for inflammation in tumor pathogenesis. A total number of 1798 unique elements were identified by a bottom-up approach with a special focus on the 433 proteins commonly characterized in the 85.7% of the samples analyzed. Their gene ontology classification evidenced the involvement of the adherence system, intermediate filaments, and actin cytoskeleton in tumor pathogenesis and of elements part of the Wnt, FGF, and EGFR signaling pathways. In addition, proteins involved in calcium modulation, innate immunity, inflammation, CCKR and integrin signaling, and gonadotropin-releasing hormone receptor pathways were also outlined. Further than confirming proteomic data previously obtained on AC intracystic fluid, these results offer a preliminary overview of the AC whole tissue protein phenotype, adding new hints towards the comprehension of this still obscure pediatric brain tumor.
Data-Driven and Precursor-Group Uncertainty Propagation of Lattice Kinetic Parameters in UAM Benchmark
Authors: Radaideh Majdi I. , Kozlowski Tomasz , Wieselquist William A. , Jessee Matthew A.
Published: May 2019

A new data-driven sampling-based framework was developed for uncertainty quantification (UQ) of the homogenized kinetic parameters calculated by lattice physics codes such as TRITON and Polaris. In this study, extension of the database for the delayed neutron data (DND) is performed by exploring more delayed neutron experiments and adding additional isotopes/actinides to the data libraries. Afterwards, the framework is utilized to obtain a deeper knowledge of the kinetic parameters’ sensitivity and uncertainty. The kinetic parameters include precursor-group-wise delayed neutron fraction (DNF) and decay constant. Input uncertainties include nuclear data (i.e., cross-sections) and DND (i.e., precursor group parameters and fractional delayed neutron yield). It is found that kinetic parameters, especially DNFs, have large uncertainties. The DNF uncertainty is driven by the cross-section uncertainties for LWR designs, while decay constant uncertainty is dominated by the DND uncertainties. The usage of correlated U-235 thermal DND in the UQ process significantly reduces the DND uncertainty contribution on the kinetic parameters. Large void fraction and presence of neutron absorber (e.g., control rod) increase the DNF uncertainty due to the hardening of neutron spectrum. High correlation between the DNF groups (β1,..,β6) is observed, while the decay constant groups (λ1,..,λ6) show weak correlation to each other and also to DNF groups. The DNF uncertainties of the dominant precursor group 4 for PWR, BWR, and VVER are about 7.5%, 9.4%, and 7.6%, respectively. The DNF uncertainty grows to larger values after fuel burnup. Kinetic parameters’ values and uncertainties provided here can be efficiently used in subsequent core calculations, point reactor kinetics, and other applications.