bluuebunny/crossref_metadata_2025 huggingface dataset:
Identification, Safety Assessment, and Antimicrobial Characteristics of Cocci Lactic Acid Bacteria Isolated from Traditional Egyptian Dairy Products
Authors: Elsaadany Khaled , EL-Sayed Abeer I. M. , Awad Sameh
Published: September 2024

The main objective of this study is to isolate and identify lactic acid bacteria (LAB) from various Egyptian dairy products, examine their antibacterial and hemolysis potential, and ensure their safety when used as starter cultures in different dairy industries. Egyptian dairy products are often made without the use of commercial starter cultures, using raw milk and artisanal methods. The most popular traditional dairy products are Laban Rayeb and Zabady, as well as the cheese varieties of Ras, Domiati, and Karish. The microbial communities used for fermentation and the diversity of lactic acid bacteria are the most important factors that can affect the quality of these products. In order to investigate the diversity of cocci lactic acid bacteria in Egyptian dairy products, 70 samples of raw or fermented milk and cheeses were collected from traditional cheese-making factories, local markets, and farmhouses located in the Delta area of Egypt. Following this, the LAB were isolated from the samples. One hundred fifty-seven isolates of Gram-positive, catalase-negative, and cocci bacterial species were identified via rep-PCR, and some isolates were confirmed using pheS and 16S rRNA gene sequencing, as follows: Streptococcus infantarius subsp. infantarius (three isolates), Enterococcus hirae (three isolates), Enterococcus faecium (ninety-six isolates), Enterococcus faecalis (forty isolates), Enterococcus durans (six isolates), Lactococcus garvieae (one isolate), Pediococcus acidilactici (seven isolates), and Lactococcus lactis subsp. Lactis (one isolate). These findings validate that five strains have strong antibacterial activity against Escherichia coli, Salmonella typhimurium, and Listeria monocytogenes, and one hundred thirty-four strains were safe for hemolysis. The five strains were selected as protective cultures, including Pediococcus acidilactici, Lactococcus lactis subsp. lactis, E. faecalis, and E. faecium.
Portable NIR Spectroscopy to Simultaneously Trace Honey Botanical and Geographical Origins and Detect Syrup Adulteration
Authors: Caredda Marco , Ciulu Marco , Tilocca Francesca , Langasco Ilaria , Núñez Oscar , Sentellas Sònia , Saurina Javier , Pilo Maria Itria , Spano Nadia , Sanna Gavino , Mara Andrea
Published: September 2024

Fraudulent practices concerning honey are growing fast and involve misrepresentation of origin and adulteration. Simple and feasible methods for honey authentication are needed to ascertain honey compliance and quality. Working on a robust dataset and simultaneously investigating honey traceability and adulterant detection, this study proposed a portable FTNIR fingerprinting approach combined with chemometrics. Multifloral and unifloral honey samples (n = 244) from Spain and Sardinia (Italy) were discriminated by botanical and geographical origin. Qualitative and quantitative methods were developed using linear discriminant analysis (LDA) and partial least squares (PLS) regression to detect adulterated honey with two syrups, consisting of glucose, fructose, and maltose. Botanical and geographical origins were predicted with 90% and 95% accuracy, respectively. LDA models discriminated pure and adulterated honey samples with an accuracy of over 92%, whereas PLS allows for the accurate quantification of over 10% of adulterants in unifloral and 20% in multifloral honey.
Investigation into the Production of Melanin from By-Products of Huangjiu Brewing
Authors: Lin Congyu , Lu Peiqi , Ma Jingqiu , Kan Tao , Han Xiao , Liu Shuangping , Ji Zhongwei , Mao Jian
Published: September 2024

Melanin is a high value bioproduct generated through the fermentation of Aureobasidium pullulans, playing a crucial role in various fields, including food, medicine, environmental protection, and materials science. However, its high production costs and low synthetic yields significantly limit its applications. Therefore, it is essential to identify high-yield strains, reduce production costs, and optimize fermentation strategies. In this study, a high melanin-yielding Aureobasidium pullulans 53LC7 was screened and identified, and the fermentation process was optimized based on melanin yield, color value, and pullulan yield. The results indicated that the melanin yield peaked at an initial pH of 6.0, temperature of 27 °C, fermentation time of 6.5 d, and inoculation quantity of 2.5%, achieving a melanin yield of 16.33 g/L. Subsequently, huangjiu lees, a byproduct of huangjiu production, was incorporated into the fermentation medium, resulting in a melanin yield of 5.91 g/L. This suggests that the Aureobasidium pullulans was not effectively utilizing huangjiu lees. To address this, we employed an adaptive evolution strategy, which increased the melanin yield to 8.72 g/L. The enhanced production was correlated with the expression of key genes, including FKS, PKS, and Cmr1. Finally, cellulase was utilized to convert the crude fibers in huangjiu lees, which were difficult to utilize, into usable substrates, while pullulanase was employed to minimize byproduct formation in the fermentation system, resulting in a melanin yield of 19.07 g/L. This study not only provides promising strains for further research but also offers valuable insights for resource production technologies.
Antioxidant Activity of Bougainvillea spectabilis Bracts as an Alternative to Nitrites in Cooked Pork Ham
Authors: Ferreira T. Alexandra , Rodriguez Jose A. , Sánchez-Ortega Irais , Lorenzo Jose M. , Santos Eva M.
Published: September 2024

In this study, the impact of incorporating Bougainvillea spectabilis powder into ham formulation as a potential color replacement for nitrites was evaluated. Three drying methods were proposed to preserve the antioxidant properties of bougainvillea: foam-mat drying, air drying, and oven drying. Antioxidant assays (DPPH, ABTS, and FRAP) assays revealed that the presence of bougainvillea powders enhanced the antioxidant properties and maintained the stability of the ham over 8 weeks of storage at 4 °C. In addition, total polyphenolic content and presence of thiobarbituric acid reactive substances (TBARS) were evaluated and showed higher and lower scores, respectively, in the samples with the incorporation of bougainvillea compared to the control samples, suggesting their potential to replace nitrite salts by providing natural antioxidant protection. Sensorial analysis also revealed no significant differences in sensory attributes in hams with 0.1% bougainvillea powder compared to nitrite samples. The incorporation of the bougainvillea powders in the ham formulation improved the sensorial attributes and consumer overall acceptance even after 8-week cold storage at 4 °C.
Numerical Simulation and Parameter Estimation of the Space-Fractional Magnetohydrodynamic Flow and Heat Transfer Coupled Model
Authors: Liu Yi , Jiang Xiaoyun , Jia Junqing
Published: September 2024

In this paper, a coupled model is built to research the space-fractional magnetohydrodynamic (MHD) flow and heat transfer problem. The fractional coupled model is solved numerically by combining the matrix function vector products method in the temporal direction with the spectral method in the spatial direction. A fast method based on the numerical scheme is established to reduce the computational time. With the help of the Bayesian method, the space-fractional orders of the coupled model are estimated, and the problem of multi-parameter estimation in the coupled model is solved. Finally, a numerical example is carried out to verify the stability of the numerical methods and the effectiveness of the parameter estimation method. Results show that the numerical method is stable, which converges with an accuracy of O(τ2+N−r). The fast method is efficient in reducing the computational time, and the parameter estimation method can effectively estimate parameters in the space-fractional coupled model. The numerical solutions are discussed to describe the effects of several important parameters on the velocity and the temperature. Results indicate that the Lorentz force produced by the MHD flow blocks the movement of the fluid and prolongs the time for the fluid to reach a stable state. But the Hall parameter m weakens this hindrance. The Joule heating effects play a negative role in heat transfer.
Slowly Rotating Peculiar Star BD00°1659 as a Benchmark for Stratification Studies in Ap/Bp Stars
Authors: Romanovskaya Anna , Ryabchikova Tatiana , Pakhomov Yury , Potravnov Ilya , Sitnova Tatyana
Published: September 2024

We present the results of a self-consistent analysis of the magnetic silicon star BD+00°1659, based on its high-resolution spectra taken from the ESPaDOnS archive (R = 68,000). This narrow-lined star shows the typical high Si abundance and Si ii– iii anomaly, making it an ideal prototype for investigating the vertical distribution of Si and Fe in the stellar atmosphere. The derived abundances, ranging from helium to lanthanides, confirm the star’s classification as a silicon Bp spectral type. Silicon and iron are represented by lines of different ionisation stages (Fe i– iii, Si i– iii), indicating an ionisation imbalance interpreted as evidence of atmospheric stratification. Our stratification analysis reveals that there is a jump in iron and silicon abundances of 1.5 dex at atmospheric layers with an optical depth of logτ5000 = −0.85–−1.00. Non-LTE calculations for iron in this stratified atmosphere show minor non-LTE effects. Our results can be applied to studying the impact of stratification on the emergent flux in rapidly rotating Si stars with similar atmospheric parameters and abundance anomalies (for example, MX TrA), where direct stratification analysis is challenging due to line blending.
Analysis of Modular Hub Genes and Therapeutic Targets across Stages of Non-Small Cell Lung Cancer Transcriptome
Authors: Barretto Angeli Joy B. , Orda Marco A. , Tsai Po-wei , Tayo Lemmuel L.
Published: September 2024

Non-small cell lung cancer (NSCLC), representing 85% of lung cancer cases, is characterized by its heterogeneity and progression through distinct stages. This study applied Weighted Gene Co-expression Network Analysis (WGCNA) to explore the molecular mechanisms of NSCLC and identify potential therapeutic targets. Gene expression data from the GEO database were analyzed across four NSCLC stages (NSCLC1, NSCLC2, NSCLC3, and NSCLC4), with the NSCLC2 dataset selected as the reference for module preservation analysis. WGCNA identified eight highly preserved modules—Cyan, Yellow, Red, Dark Turquoise, Turquoise, White, Purple, and Royal Blue—across datasets, which were enriched in key pathways such as “Cell cycle” and “Pathways in cancer”, involving processes like cell division and inflammatory responses. Hub genes, including PLK1, CDK1, and EGFR, emerged as critical regulators of tumor proliferation and immune responses. Estrogen receptor ESR1 was also highlighted, correlating with improved survival outcomes, suggesting its potential as a prognostic marker. Signature-based drug repurposing analysis identified promising therapeutic candidates, including GW-5074, which inhibits RAF and disrupts the EGFR–RAS–RAF–MEK–ERK signaling cascade, and olomoucine, a CDK1 inhibitor. Additional candidates like pinocembrin, which reduces NSCLC cell invasion by modulating epithelial-mesenchymal transition, and citalopram, an SSRI with anti-carcinogenic properties, were also identified. These findings provide valuable insights into the molecular underpinnings of NSCLC and suggest new directions for therapeutic strategies through drug repurposing.
Health Attitudes of Women Living in Religious Communities—A Preliminary Study
Authors: Teodorczyk Paulina , Najechalski Paweł , Walędziak Maciej , Różańska-Walędziak Anna
Published: September 2024

Introduction: Members of consecrated life communities exhibit homogeneity with regard to factors that are relevant to health, including diet, daily activities, religiosity, and housing. This may be indicative of the manner in which the community influences the formation of the health-seeking attitudes of its members. Purpose of the study: The objective of this study was to validate a survey about health awareness and health-seeking attitudes among consecrated people and to identify potential issues to be improved. In addition, the study aspires to provide insights into the lifestyles of consecrated persons in Poland, based on the results of the survey and available data. Materials and Methods: The study group included 27 female participants, members of societies of apostolic life and non-habitual apostolic religious congregations of the Roman Catholic Church. The participants were invited to express their comments and suggestions on the survey, with the purpose of validating the survey before using it as a tool for a larger study. Results: All participants agreed that nutrition, rest, and physical activity had a significant impact on health. A total of 89% of participants considered their lifestyle as healthy and a similar proportion indicated that living in a consecrated community had a positive impact on their health, with the most positive influence of factors related to spiritual exercise, rhythm of the day, and sense of interpersonal connection within the community. Approximately 44% of participants indicated that their attitude was based on a healthy diet and adequate sleep quality. However, they identified an existing need to improve the balance between work and rest. Additionally, 4% of respondents admitted irregular eating habits, ignoring quality of alimentary products, non-satisfactory rest time, and insufficient sleep. Conclusions: Even though the purpose of this study was only to validate a survey predestined for a larger study, it already gives an insight into the level of awareness of health behavior and lifestyle of residents of religious communities.
Innovative Chair and System Designs to Enhance Resistance Training Outcomes for the Elderly
Authors: Qi Teng , Iwamoto Miyuki , Choi Dongeun , Panote Siriaraya , Kuwahara Noriaki
Published: September 2024

Introduction: This study aims to provide a safe, effective, and sustainable resistance training environment for the elderly by modifying chairs and movement systems used during training, particularly under unsupervised conditions. Materials and Methods: The research focused on investigating the effect of modified chair designs on enhancing physical stability during resistance training by involving 19 elderly participants (mean 72.1, SD 4.7). The study measured changes in the body’s acceleration during movements to compare the effectiveness of the modified chairs with those commonly used in chair-based exercise (CBE) training in maintaining physical stability. A system was developed based on experimental video data, which leverages MediaPipe to analyze the videos and compute joint angles, identifying whether the actions are executed correctly. Results and Conclusions: Comparisons revealed that modified chairs offered better stability during sitting (p < 0.001) and stand-up (p < 0.001) resistance training. According to the questionnaire survey results, compared to the regular chair without an armrest, the modified chair provided a greater sense of security and a better user experience for the elderly. Video observations indicated that the correct completion rate for most exercises, except stand-up resistance training, was only 59.75%, highlighting the insufficiency of modified chairs alone in ensuring accurate movement execution. Consequently, the introduction of an automatic system to verify proper exercise performance is essential. The model developed in this study for recognizing the correctness of movements achieved an accuracy rate of 97.68%. This study proposes a new chair design that enhances physical stability during resistance training and opens new avenues for utilizing advanced technology to assist the elderly in their training.
Exploring the Relationship between Health Literacy and Depression among Individuals with Chronic Conditions: Insights from the KCHS
Authors: Kim Eungyeong
Published: September 2024

Objectives: This study, utilizing data from the 2021 Korean Community Health Survey (KCHS), aimed to investigate the role of health literacy in mitigating depression among individuals with chronic diseases, particularly focusing on how sociodemographic and subjective factors influence this relationship. Methods: With a sample of 77,133 subjects primarily dealing with hypertension and diabetes, the study employed various analytical tools to identify factors associated with an increased risk of depression. Results: These factors included marital status (OR 1.47, 95% CI 1.40–1.54), residential district (OR 1.43, 95% CI 1.39–1.50), unemployment (OR 1.47, 95% CI 1.41–1.54), enrollment in the national basic living security program (OR 1.50, 95% CI 1.40–1.62), unmet healthcare needs (OR 2.32, 95% CI 2.14–2.51), fair subjective health status (OR 1.74, 95% CI 1.63–1.90), poor subjective health status (OR 4.33, 95% CI 4.05–4.63), and stress (OR 4.56, 95% CI 4.36–4.77). Notably, individuals with higher health literacy showed a significantly lower susceptibility to depression (OR 0.74, 95% CI 0.68–0.75), underscoring the importance of health literacy as a critical factor in mental health outcomes. Conclusions: Such initiatives could serve as effective preventive measures against depression in this vulnerable population.
In the Shadow of the Pandemic: Examining Therapists’ Perceptions of Work-Related Stress in the Late Stages of the COVID-19 Pandemic in Germany
Authors: Johann Smilla , Evans Megan , Böttcher Rike , Muller Nadine , Buchberger Barbara , El Bcheraoui Charbel , Weishaar Heide
Published: September 2024

Background/Objectives: The previous literature has identified increased work-related stress among healthcare workers (HCWs) during the COVID-19 pandemic. This study analyzes work-related perceived stress experienced by therapists during the COVID-19 pandemic in Germany to identify potential for supporting this crucial group of HCWs in future health crises. Methods: Survey data on stress, measured through the Perceived Stress Scale 4, among HCWs (therapists n = 612, nurses n = 501, and doctors n = 461) were analyzed using descriptive statistics, and data from four semi-structured interviews and seven focus group discussions with therapists were analyzed using thematic content analysis. Data were collected from March to September 2022. Results: Therapists reported similar stress levels to other HCWs, with the reporting of perceived stress differing between work contexts. Eight stressors were identified through the thematic analysis: (1) concerns about maintaining the quality of care, (2) uncertainty about the future, (3) workload, (4) interactions with colleagues and patients, (5) PPE, (6) the risk of infection, (7) insufficient information flow, and (8) the lack of public and political recognition. Conclusions: This study emphasizes the distinct challenges that therapists experienced during the COVID-19 pandemic. By identifying the factors that contributed to the stress experienced, the study can inform targeted support strategies which can enhance therapists’ work, ultimately contributing to sustaining essential healthcare services during public health crises.
Diabetes Distress and Health-Related Quality of Life among Patients with Type 2 Diabetes—Mediating Role of Experiential Avoidance and Moderating Role of Post-Traumatic Growth
Authors: Aliche Chinenye Joseph , Idemudia Erhabor Sunday
Published: September 2024

Objectives: Many people living with type 2 diabetes experience diabetes distress which impacts negatively on their health-related quality of life (HRQoL). However, little is known about the modifiable factors or psychological processes that make this happen. The current study examines the role of experiential avoidance and post-traumatic growth (PTG) in association with diabetes distress and the HRQoL. Methods: Participants included 303 patients with type 2 diabetes conveniently selected from two tertiary healthcare institutions in Nigeria. They completed relevant self-report measures. The Hayes PROCESS macro for SPSS was used for data analysis. Results: The results showed that experiential avoidance mediated the association between diabetes distress and the HRQoL [95% CI: −0.15, −0.07]. PTG significantly moderated the association between diabetes distress and the HRQoL [95% CI: 0.01, 0.02]. Specifically, diabetes distress was associated with a poor HRQoL only among patients with low levels of PTG but not among those with average and high levels of PTG. Conclusion: These findings underscore the importance of Acceptance and Commitment Therapy as it can potentially decrease the experiential avoidance behaviour of patients. Moreover, intervention should also target the facilitation of PTG due to its beneficial effects in reducing the negative effects of diabetes distress on health and recovery.
Correction: Heumann et al. Study Conditions and University Students’ Mental Health during the Pandemic: Results of the COVID-19 German Student Well-Being Study (C19 GSWS). Int. J. Environ. Res. Public Health 2023, 20, 5286
Authors: Heumann Eileen , Trümmler Jannis , Stock Christiane , Helmer Stefanie M. , Busse Heide , Negash Sarah , Pischke Claudia R.
Published: September 2024

There was an error in the original publication [...]
Sharing Your Husband: Adult Attachment Styles and Emotional Responses of Israeli Bedouin-Arab Women to Potential Polygynous Marriage
Authors: Alhuzail Nuzha Allassad , Besser Avi , Zeigler-Hill Virgil
Published: September 2024

Polygynous marriage is prevalent among Israel’s Bedouin-Arab community, despite being explicitly banned by state law. Cultural traditions and customs permit men to take multiple wives, and Islamic teachings do not prohibit the practice. The impact of polygyny is significant, affecting women, children, and society as a whole in various ways. We examined the connections that attachment anxiety and attachment avoidance had with hypothetical responses to the potential threat of polygynous marriages in a community sample of young unmarried Israeli Bedouin-Arab women (n = 306). Participants were randomly assigned to imagine either a high-threat scenario (i.e., finding that their husband decided to take a second wife; n = 151) or a low-threat scenario (i.e., their husband would not decide to take a second wife; n = 155) and report their anticipated responses to these scenarios. Results showed that attachment anxiety was positively associated with anticipated negative emotional responses in the high-threat condition but not in the low-threat condition. However, neither attachment anxiety nor attachment avoidance were associated with the perceived threat of polygyny to their marriage. The findings of this study suggest that attachment styles play a significant role in shaping women’s perceptions and reactions to hypothetical polygynous marriages. Women with anxious attachment styles are more likely to experience intensified negative emotions regarding such marriages, while those with avoidant attachment styles may display greater tolerance toward polygyny. These results highlight the need for tailored interventions and support systems that take into account individual psychological profiles as well as the broader cultural context of Israeli Bedouin-Arab society.
“Private Hospitals Generally Offer Better Treatment and Facilities”: Out-of-Pocket Expenditure on Healthcare and the Preference for Private Healthcare Providers in South India
Authors: Kamath Sagarika , Poojary Mahalakshmi , Shetty Harshith , Umesh Kshithija , Kar Soham , Lakshmi Ramesh Vani , Hajare Gaurav , Thomas Albi , Brand Helmut , Jahangir Selim , Kamath Rajesh
Published: September 2024

Out-of-pocket expenditure (OOPE) directly reflects households’ financial burden for healthcare. Despite efforts to enhance accessibility and affordability through government initiatives and insurance schemes, OOPE remains problematic, especially in rural areas with inadequate public healthcare infrastructure. This study examines factors influencing OOPE in Karnataka’s Dakshina Kannada, Udupi, and Shimoga districts, investigating socioeconomic characteristics, healthcare infrastructure, and accessibility to inform policies for equitable healthcare access and reduced household financial strain. Using purposive sampling, 61 semi-structured interviews were conducted in rural and urban South Karnataka, recorded in Kannada after obtaining consent, and thematically analyzed. Results revealed mixed perceptions of healthcare quality, cost, and accessibility between government and private hospitals. Government facilities were lauded for improved infrastructure and affordability, while private hospitals were preferred for quality and personalized care despite higher costs. Health insurance significantly impacted OOPE reduction. Participants emphasized the need for increased awareness of government insurance programs and improved quality in public hospitals. The study concludes that private hospitals are favored for superior care despite expenses, while government hospitals are valued for affordability. Expanding insurance coverage and improving public awareness are crucial for enhancing healthcare accessibility and affordability.
Pre-Dam Vltava River Valley—A Case Study of 3D Visualization of Large-Scale GIS Datasets in Unreal Engine
Authors: Janovský Michal
Published: September 2024

This article explores the potential of the Unreal Engine as a tool for creating realistic 3D visualizations of large-scale areas (1000 km2 and more) using GIS datasets. Unlike small-scale visualizations, large-scale visualizations are rare and often not public, which presents significant problems since they present different challenges and require different approaches. This article presents several relevant scientific studies and projects that have successfully used game engines for similar purposes. This case study focuses on the computational techniques used in Unreal Engine for the 3D visualization of GIS data and the potential application of Unreal Engine in large-scale geo-visualizations. It explores the potential for using GIS data within a game engine, including plug-ins that provide additional functionality for working with GIS data, such as the Vitruvio plug-in to implement procedural modeling of buildings. The case study is applied to GIS datasets of the historical Vltava Valley covering an area of 1670 km2 to demonstrate the unique challenges of using Unreal Engine to create realistic visualizations of large-scale historical landscapes. The resulting visualizations are presented. The practical application of this research provides insights into the potential of the Unreal Engine as a tool for creating realistic 3D visualizations of large-scale historical areas.
Sky-Scanning for Energy: Unveiling Rural Electricity Consumption Patterns through Satellite Imagery’s Convolutional Features
Authors: Huang Yaofu , Xu Weipan , Chen Dongsheng , Li Qiumeng , Deng Weihuan , Li Xun
Published: September 2024

The pursuit of the Sustainable Development Goals has highlighted rural electricity consumption patterns, necessitating innovative analytical approaches. This paper introduces a novel method for predicting rural electricity consumption by leveraging deep convolutional features extracted from satellite imagery. The study employs a pretrained remote sensing interpretation model for feature extraction, streamlining the training process and enhancing the prediction efficiency. A random forest model is then used for electricity consumption prediction, while the SHapley Additive exPlanations (SHAP) model assesses the feature importance. To explain the human geography implications of feature maps, this research develops a feature visualization method grounded in expert knowledge. By selecting feature maps with higher interpretability, the “black-box” model based on remote sensing images is further analyzed and reveals the geographical features that affect electricity consumption. The methodology is applied to villages in Xinxing County, Guangdong Province, China, achieving high prediction accuracy with a correlation coefficient of 0.797. The study reveals a significant positive correlations between the characteristics and spatial distribution of houses and roads in the rural built environment and electricity demand. Conversely, natural landscape elements, such as farmland and forests, exhibit significant negative correlations with electricity demand predictions. These findings offer new insights into rural electricity consumption patterns and provide theoretical support for electricity planning and decision making in line with the Sustainable Development Goals.
Evaluation of Volatile Organic Compounds from Spotted Lanternfly (Lycorma delicatula) Eggs Using Headspace Odor Sampling Methods
Authors: Cantu Ariela , Aviles-Rosa Edgar O. , Hall Nathaniel J. , Prada-Tiedemann Paola A.
Published: September 2024

The spotted lanternfly (SLF) is an invasive species native to China. It was first discovered in the United States in Pennsylvania in 2014. It is known to cause great economic damage by destroying various crops, specifically grape vines, and therefore, several efforts have been made to control and mitigate its spread from the Northeast. Canine detection is a useful detection tool; however, it is crucial to understand the volatile organic compounds emitting by this pest to better direct canine training paradigms to prevent false alerts and to understand potential volatile markers of importance indicative of this species. The purpose of this study is to address the gap in research regarding the volatile organic compound (VOC) profile of SLF to better inform pest control mitigation strategies. Instrumental analysis was performed utilizing SPME-GC/MS on cold-killed SLF eggs, dried crickets, and tree bark. Differences in detected VOCs within each sample set depicted distinctive odor profiles for each matrix tested. Storage of these samples also depicted VOC accumulation variation as a function of time, thereby providing implications for long-term storage and sample handling for these types of training aids in canine applications.
RNAseq-Based Carboxylesterase Nl-EST1 Gene Expression Plasticity Identification and Its Potential Involvement in Fenobucarb Resistance in the Brown Planthopper Nilaparvata lugens
Authors: Khan Murtaza , Han Changhee , Choi Nakjung , Kim Juil
Published: September 2024

Carbamate insecticides have been used for over four decades to control brown planthopper, Nilaparvata lugens, but resistance has been reported in many countries, including the Republic of Korea. The bioassay results on resistance to fenobucarb showed that the LC50 values were 3.08 for the susceptible strain, 10.06 for the 2015 strain, and 73.98 mg/L for the 2019 strain. Compared to the susceptible strain, the 2015 and 2019 strains exhibited resistance levels 3.27 and 24.02 times higher, respectively. To elucidate the reason for the varying levels of resistance to fenobucarb in these strains, mutations in the acetylcholinesterase 1 (ACE1) gene, the target gene of carbamate, were investigated, but no previously reported mutations were confirmed. Through RNA-seq analysis focusing on the expression of detoxification enzyme genes as an alternative resistance mechanism, it was found that the carboxylesterase gene Nl-EST1 was overexpressed 2.4 times in the 2015 strain and 4.7 times in the 2019 strain compared to the susceptible strain. This indicates a strong correlation between the level of resistance development in each strain and the expression level of Nl-EST1. Previously, Nl-EST1 was reported in an organophosphorus insecticide-resistant strain of Sri Lanka 2000. Thus, Nl-EST1 is crucial for developing resistance to organophosphorus and carbamate insecticides. Resistance-related genes such as Nl-EST1 could serve as expression markers for resistance diagnosis, and can apply to integrated resistance management of N. lugens.
Mechanical versus Biological Valve Prostheses for Infective Endocarditis Presenting with Stroke
Authors: Kahrovic Amila , Angleitner Philipp , Herkner Harald , Werner Paul , Andreeva Alexandra , Poschner Thomas , Laengle Severin , Kocher Alfred , Laufer Guenther , Andreas Martin
Published: September 2024

Objectives: This study aimed to compare the clinical outcomes of mechanical and biological valve prostheses in patients with infective endocarditis presenting with stroke. Methods: Ninety-five adults with infective endocarditis complicated by stroke at baseline who underwent aortic and/or mitral valve replacement were analyzed retrospectively. The primary outcome was a composite outcome of all-cause mortality, ischemic stroke, hemorrhagic stroke, and re-endocarditis. Secondary outcomes included the individual components of the composite outcome and modified Rankin scale deterioration during follow-up. Results: Among the study cohort, 34 patients (35.8%) received mechanical valve prostheses and 61 (64.2%) received biological valve prostheses. Implantation of a mechanical valve prosthesis seems to be associated with a decreased risk of attaining the composite outcome (adjusted HR 0.46, 95% CI 0.22–0.96, and p = 0.037). Analyses of the individual components of the composite outcome showed that implantation of a mechanical valve prosthesis might not be associated with an increased risk of ischemic stroke, hemorrhagic stroke, and all-cause mortality during the follow-up period. Further, the risk of re-endocarditis was significantly lower in recipients of a mechanical valve prosthesis (adjusted HR 0.15, 95% CI 0.06–0.77, p = 0.026). Notably, a trend toward decreased risk of modified Rankin scale deterioration throughout the follow-up period was observed in this group (adjusted odds ratio 0.22, 95% CI 0.05–1.02, p = 0.053). Conclusions: Implantation of mechanical valve prostheses in patients presenting with infective endocarditis complicated by stroke seems to be beneficial in terms of a reduced risk of experiencing a composite outcome. Analyses of larger cohorts are required to validate our findings.
Peritubular and Tubulointerstitial Inflammation as Predictors of Impaired Viral Clearance in Polyomavirus Nephropathy
Authors: Omić Haris , Eder Michael , Schrag Tarek A. , Kozakowski Nicolas , Kläger Johannes , Bond Gregor , Kikić Željko
Published: September 2024

Introduction: Polyomavirus-associated nephropathy (BKPyVAN) is a common complication in kidney transplant recipients. The histological changes in the context of BKPyVAN and their association with the viral load and outcomes are still being investigated. Methods: This retrospective study involved 100 adult patients transplanted between 2000 and 2021, with available archived biopsy slides, aiming to analyze associations between viral load clearance in the blood (reduction in BKPyVAN-DNAemia below detection level) and histological features in biopsy-proven BKPyVAN. A kidney pathologist blinded to the clinical data reassessed the BANFF 2019 lesion scores in the BKPyVAN index biopsy. The primary endpoint was viral clearance three months after the diagnosis. Results: The presence of tubulointerstitial inflammation, peritubular capillaritis, and higher PVN Class at the diagnosis was linked to a reduced likelihood of viral clearance three months later (interstitial inflammation OR = 0.2, 95% CI [0.07–0.55], tubulitis OR = 0.39, 95% CI [0.21–0.73], peritubular capillaritis OR = 0.25, 95% CI [0.08–0.82], PVN Score OR = 0.1, 95% CI [0.03–0.4]), independently of other covariates. Combining the four lesions using the ROC analysis enhanced their capability to predict persistent BK viremia after 3 months with an AUC of 0.94. Conclusions: The presence of interstitial inflammation, tubulitis, and peritubular capillaritis, as well as the higher PVN Score, was associated with an up to 90% lower likelihood of viral load clearance three months post-diagnosis. These findings underscore the importance of histological evaluation as a surrogate of subsequent viral clearance and offer valuable insights for the management of BKPyVAN.
Clinical Assessment and Management of Acute Spinal Cord Injury
Authors: Quinones Christian , Wilson John Preston , Kumbhare Deepak , Guthikonda Bharat , Hoang Stanley
Published: September 2024

The information contained in this article is suitable for clinicians practicing in the United States desiring a general overview of the assessment and management of spinal cord injury (SCI), focusing on initial care, assessment, acute management, complications, prognostication, and future research directions. SCI presents significant challenges, affecting patients physically, emotionally, and financially, with variable recovery outcomes ranging from full functionality to lifelong dependence on caregivers. Initial care aims to minimize secondary injury through thorough neurological evaluations and imaging studies to assess the severity of the injury. Acute management prioritizes stabilizing respiratory and cardiovascular functions and maintaining proper spinal cord perfusion. Patients with unstable or progressive neurological decline benefit from timely surgical intervention to optimize neurological recovery. Subacute management focuses on addressing common complications affecting the respiratory, gastrointestinal, and genitourinary systems, emphasizing a holistic, multidisciplinary approach. Prognostication is currently based on neurological assessments and imaging findings, but emerging biomarkers offer the potential to refine outcome predictions further. Additionally, novel therapeutic interventions, such as hypothermia therapy and neuroprotective medications are being explored to mitigate secondary damage and enhance recovery. This paper serves as a high-yield refresher for clinicians for the assessment and management of acute spinal cord injury during index admission.
Novel Immune Checkpoint Inhibitor Targets in Advanced or Metastatic Renal Cell Carcinoma: State of the Art and Future Perspectives
Authors: Compagno Samuele , Casadio Chiara , Galvani Linda , Rosellini Matteo , Marchetti Andrea , Tassinari Elisa , Piazza Pietro , Mottaran Angelo , Santoni Matteo , Schiavina Riccardo , Massari Francesco , Mollica Veronica
Published: September 2024

Immune checkpoint inhibitors (ICI) have become the cornerstone of treatment in renal cell carcinoma (RCC), for both metastatic disease and in an adjuvant setting. However, an adaptive resistance from cancer cells may arise during ICI treatment, therefore many studies are focusing on additional immune checkpoint inhibitor pathways. Promising targets of immunotherapeutic agents under investigation include T cell immunoglobulin and ITIM domain (TIGIT), immunoglobulin-like transcript 4 (ILT4), lymphocyte activation gene-3 (LAG-3), vaccines, T cell immunoglobulin and mucin domain-containing protein 3 (TIM-3), and chimeric antigen receptor (CAR) T cells. In this review of the literature, we recollect the current knowledge of the novel treatment strategies in the field of immunotherapy that are being investigated in RCC and analyze their mechanism of action, their activity and the clinical studies that are currently underway.
Cyclic Fatigue Resistance of Four Heat-Treated Nickel-Titanium Files in Severely Curved Simulated Canals: An In Vitro Study
Authors: Greco Katia , Paolone Gaetano , Cicero Giuseppe , Tetè Giulia , Cantile Nicola , Sberna Maria Teresa , Saladino Teresa , Gherlone Enrico Felice , Cantatore Giuseppe
Published: September 2024

Background: Rotary Ni-Ti files are susceptible to sudden intra-canal separation due to cyclic fatigue stress, particularly in curved canals. To increase resistance to cyclic fatigue, new heat-treated files have been introduced. This study aimed to compare the performance of four heat-treated Ni-Ti files in two simulated curved root canals by evaluating the effect of the alloy, rotation speed, and diameter of the files on their resistance to cyclic fatigue. Methods: The Ni-Ti files included in the study were the ProTaper Gold® (Dentsply Sirona) F2, ProTaper Ultimate® (Dentsply Sirona) F2, FQ® (Komet) 25.06, and Blueshaper® (Zarc4Endo) Z4 25.06. Two groups of 30 files were selected for each system and were tested in two simulated canals milled in a specific metal template. One group was tested in a 60° curved canal and the other in a 90° curved canal. Results: In the 60° simulated canal, there were no instrument fractures within the 15 min time limit. In the 90° simulated canal, the Blueshaper Z4 demonstrated a lower resistance to cyclic fatigue, while FQ 25.06 showed statistically higher fatigue resistance based on both the Kruskal–Wallis and Games–Howell tests (p < 0.05). Conclusions: No differences were found between files when tested in a 60° curved canal for up to 15 min. However, in a 90° canal, the FQ® files showed significantly higher resistance to cyclic fatigue, especially compared to the Blueshaper® Z4. The ProTaper Ultimate and ProTaper Gold produced intermediate results, with the ProTaper Ultimate F2 slightly outperforming the ProTaper Gold F2.
Microfilm Coatings: A Biomaterial-Based Strategy for Modulating Femoral Deflection
Authors: Olivares-Hernandez Ana Elisabeth , Olivares-Robles Miguel Angel , Méndez-Méndez Juan Vicente , Gutiérrez-Camacho Claudia
Published: September 2024

Wear on the surface of the femoral head increases the risk of hip and femur fractures. Biomechanical experiments conducted on the femur are based on its bending and torsional rigidities. Studies regarding the deflection of the femur bone when the femoral head is coated with microfilms composed of durable and compatible biomaterials are poor. This study aimed to investigate the effects of different biomaterial microfilm coatings over the femoral head on the deflection of the human femur. We utilized 2023 R1 finite element analysis (FEA) software to model the directional deformation on the femoral head and examine the femur’s deflection with varying microfilm thicknesses. The deflection of the femur bone was reported when the femoral head was uncoated and coated with titanium, stainless steel, and pure gold microfilms of different thicknesses (namely, 50, 75, and 100 μm). Our results show that the femur’s minimum and maximum deflection occurred for stainless steel and gold, respectively. The deformation of the femur was lower when the femoral head was coated with a 50-micrometer microfilm of stainless steel, compared to the deformation obtained with gold and titanium. When the thickness of the microfilm for each of the materials was increased, the deformation continued to decrease. The minimum deformation of the femur occurred for a thickness of 100 μm with stainless steel, followed by titanium and gold. The difference in the directional deformation of the femur between the materials was more significant when the coating was 100 μm, compared to the thicknesses of 50 and 75 μm. The findings of this study are expected to significantly contribute to the development of advanced medical techniques to enhance the quality of life for patients with femur bone-related issues. This information can be used to develop more resilient coatings that can withstand wear and tear.
Evaluation of Focus Measures for Hyperspectral Imaging Microscopy Using Principal Component Analysis
Authors: Nasibov Humbat
Published: September 2024

An automatic focusing system is a crucial component of automated microscopes, adjusting the lens-to-object distance to find the optimal focus by maximizing the focus measure (FM) value. This study develops reliable autofocus methods for hyperspectral imaging microscope systems, essential for extracting accurate chemical and spatial information from hyperspectral datacubes. Since FMs are domain- and application-specific, commonly, their performance is evaluated using verified focus positions. For example, in optical microscopy, the sharpness/contrast of visual peculiarities of a sample under testing typically guides as an anchor to determine the best focus position, but this approach is challenging in hyperspectral imaging systems (HSISs), where instant two-dimensional hyperspectral images do not always possess human-comprehensible visual information. To address this, a principal component analysis (PCA) was used to define the optimal (“ideal”) optical focus position in HSIS, providing a benchmark for assessing 22 FMs commonly used in other imaging fields. Evaluations utilized hyperspectral images from visible (400–1100 nm) and near-infrared (900–1700 nm) bands across four different HSIS setups with varying magnifications. Results indicate that gradient-based FMs are the fastest and most reliable operators in this context.
Process Developments in Electron-Beam Powder Bed Fusion Enabled by Near-Infrared Radiation
Authors: Sjöström William , Rännar Lars-Erik , Botero Carlos , Membrado Laia Ortiz
Published: September 2024

The use of an electron beam (EB) as a heating source in EB-based powder bed fusion (PBF-EB) has several limitations, such as reduced powder recyclability, short machine service intervals, difficulties with heating large areas and the limited processability of charge-sensitive powders. Near-infrared (NIR) heating was recently introduced as a feasible replacement and/or complement to EB heating in PBF-EB. This work further investigates the feasibility of using NIR to eliminate the need for a build platform as well as to enable easier repairing of parts in PBF-EB. NIR-assisted Ti-6Al-4V builds were successfully carried out by starting from a loose powder bed without using a build platform. The results do not only confirm that it is possible to eliminate the build platform by the aid of NIR, but also that it can be beneficial for the process cleanliness and improve the surface quality of built parts. Furthermore, a 430 stainless-steel (SS) component could be repaired by positioning it in a loose 316L SS powder bed using a fully NIR-heated PBF-EB process.
Do Differences in Modes of Production Affect the Ability of Ecological Restoration Projects to Improve Local Livelihoods?
Authors: Xiao Bei , Zhang Dongying , Li Renjun
Published: September 2024

Large ecological restoration projects have been widely implemented across the world since the 20th century, yielding complex ecological, economic, and social results. Today, balancing ecological restoration with local people’s livelihoods is a key issue. Based on the existing literature, this study proposes a “shock adaptation” mechanism to describe the response of rural residents’ livelihoods to the impact of ecological restoration projects. We hypothesize that adaptability varies across the modes of production. To verify our hypothesis, we used the machine-learning-based local projection (LP) method to analyze China’s Three-North Shelter Forest Program (TNSFP), with data for 596 counties from 2001 to 2020. After the TNSFP started, rural residents’ income dropped, rose, and then exceeded the starting point over 8 years. Moreover, significant heterogeneity exists between agricultural and pastoral areas. Agricultural areas recover faster and improve livelihoods, while pastoral areas take longer to bounce back. The results confirmed the “shock adaptation” mechanism and suggested the importance of the mode of production. Policymakers should add more social–ecological indicators to their evaluation systems, allow local communities more self-management, and offer extra help to those struggling to recover from shocks.
4-(10-Phenyl-9-Anthracenyl)-1,2-Benzenediol
Authors: Edwards Nicola , Harris Kelsey
Published: September 2024

The title compound, 4-(10-phenyl-9-anthracenyl)-1,2-benzenediol, was synthesized using a two-step protocol. In the first step, 9-phenyl,10-bromoanthracene was coupled to 3,4-dimethoyphenylboronic acid by employing Pd(PPh3)4 as the catalyst and potassium carbonate as the base. Methoxy group removal was effected using HBr in the presence of acetic acid in the second step. Overall, two novel 9,10-diphenylanthracence-based compounds were synthesized in this work; standard spectroscopic techniques and high-resolution mass spectrometry were employed in their characterization. The photophysical properties of these compounds are also reported. These compounds are potentially useful as sensors, catalysts and building blocks for larger architectures.