bluuebunny/crossref_metadata_2025 huggingface dataset:
Cloning, Characterization and Expression of the Phenylalanine Ammonia-Lyase Gene (PaPAL) from Spruce Picea asperata
Authors: Liu Yufeng , Liu Lijuan , Yang Shuai , Zeng Qian , He Zhiran , Liu Yinggao
Published: July 2019

Phenylalanine ammonia-lyase (PAL) is the crucial enzyme of the phenylpropanoid pathway, which plays an important role in plant disease resistance. To understand the function of PAL in Picea asperata, in this study, the full-length cDNA sequence of the PAL gene from this species was isolated and named PaPAL. The gene contains a 2160-bp open reading frame (ORF) encoding 720 amino acids with a calculated molecular weight of 78.7 kDa and a theoretical isoelectric point of 5.88. The deduced PaPAL protein possesses the specific signature motif (GTITASGDLVPLSYIA) of phenylalanine ammonia-lyases. Multiple alignment analysis revealed that PaPAL has high identity with other plant PALs. The tertiary structure of PaPAL was predicted using PcPAL from Petroselinum crispum as a template, and the results suggested that PaPAL may have a similar function to that of PcPAL. Furthermore, phylogenetic analysis indicated that PaPAL has a close relationship with other PALs from the Pinaceae species. The optimal expression condition of recombinant PaPAL in Escherichia coli BL21 (DE3) was 0.2 mM IPTG (isopropyl β-D-thiogalactoside) at 16 °C for 4 h, and the molecular weight of recombinant PaPAL was found to be approximately 82 kDa. Recombinant PaPAL was purified and exhibited high PAL activity at optimal conditions of pH 8.6 and 60 °C. Quantitative real-time PCR (qRT-PCR) showed the PaPAL gene to be expressed in all tissues of P. asperata tested, with the highest expression level in the needles. The PaPAL gene was induced by the pathogen (Lophodermium piceae), which caused needle cast disease, indicating that it might be involved in defense against needle cast disease. These results provide a basis for understanding the molecular mechanisms of the PAL gene in the process of P. asperata disease resistance.
Enological Repercussions of Non-Saccharomyces Species
Authors: Aranda Agustín
Published: July 2019

The bulk of the sugar fermentation in grape juice, in order to produce wine is carried out by yeasts of the genus Saccharomyces, mainly S [...]
The Development of a Sorghum Bran-Based Biorefining Process to Convert Sorghum Bran into Value Added Products
Authors: Makanjuola Oyenike , Greetham Darren , Zou Xiaoyan , Du Chenyu
Published: July 2019

Sorghum bran, a starch rich food processing waste, was investigated for the production of glucoamylase in submerged fungal fermentation using Aspergillus awamori. The fermentation parameters, such as cultivation time, substrate concentration, pH, temperature, nitrogen source, mineral source and the medium loading ratio were investigated. The glucoamylase activity was improved from 1.90 U/mL in an initial test, to 19.3 U/mL at 10% (w/v) substrate concentration, pH 6.0, medium loading ratio of 200 mL in 500 mL shaking flask, with the addition of 2.5 g/L yeast extract and essential minerals. Fermentation using 2 L bioreactors under the optimum conditions resulted in a glucoamylase activity of 23.5 U/mL at 72 h, while further increase in sorghum bran concentration to 12.5% (w/v) gave an improved gluco-amylase activity of 37.6 U/mL at 115 h. The crude glucoamylase solution was used for the enzymatic hydrolysis of the sorghum bran. A sorghum bran hydrolysis carried out at 200 rpm, 55 °C for 48 h at a substrate loading ratio of 80 g/L resulted in 11.7 g/L glucose, similar to the results obtained using commercial glucoamylase. Large-scale sorghum bran hydrolysis in 2 L bioreactors using crude glucoamylase solution resulted in a glucose concentration of 38.7 g/L from 200 g/L sorghum bran, corresponding to 94.1% of the theoretical hydrolysis yield.
Pasta Enrichment with an Amaranth Hydrolysate Affects the Overall Acceptability while Maintaining Antihypertensive Properties
Authors: Valdez-Meza Eduardo Enrique , Raymundo Anabela , Figueroa-Salcido Oscar Gerardo , Ramírez-Torres Giovanni Isaí , Fradinho Patrícia , Oliveira Sonia , de Sousa Isabel , Suárez-Jiménez Miroslava , Cárdenas-Torres Feliznando Isidro , Islas-Rubio Alma Rosa , Rodríguez-Olibarría Guillermo , Ontiveros Noé , Cabrera-Chávez Francisco
Published: July 2019

Background: Alcalase-treated amaranth proteins generate angiotensin-1-converting enzyme (ACE-1) inhibitory peptides, which could be useful for functional foods development. Our aim was to evaluate the technological, sensory, and antihypertensive properties of pasta enriched with an amaranth hydrolysate. Methods: Pasta with 11% (A; control), 15% (B), and 20% (C) of protein content were formulated. Pastas B and C were supplemented with an alcalase-treated amaranth protein concentrate. Cooking time, cooking lost, color, and texture were assessed. An untrained panel (n = 30) evaluated sensory attributes. The antihypertensive effect was evaluated in hypertensive rats. Results: The hydrolysate IC50 was 0.014 mg/mL. Optimum cooking time and cooking loss decreased in products B and C vs. A (p < 0.05). The L* values decreased in pasta C. Firmness increased in pasta C vs. A (p < 0.05). Adhesiveness was different among groups (p < 0.05). Pasta A had the highest acceptability (p < 0.05). The products B and C, and captopril (positive control) showed antihypertensive properties after 3 h of supplementation (p < 0.05). This effect remained after 7 h, 8 h, or 9 h. Conclusions: The addition of amaranth hydrolysates to pasta negatively impacts on the overall acceptability and, to a lesser extent, on pasta taste. However, it is possible to maintain the antihypertensive properties of the supplemented pasta under physiological conditions.
The Application of Combined Pre-Treatment with Utilization of Sonication and Reduced Pressure to Accelerate the Osmotic Dehydration Process and Modify the Selected Properties of Cranberries
Authors: Nowacka Malgorzata , Wiktor Artur , Dadan Magdalena , Rybak Katarzyna , Anuszewska Aleksandra , Materek Lukasz , Witrowa-Rajchert Dorota
Published: July 2019

The aim of this study was to investigate the effect of a pretreatment, performed by a combined method based on blanching, ultrasound, and vacuum application, on the kinetics of osmotic dehydration and selected quality properties such as water activity, color, and bioactive compound (polyphenols, flavonoids, and anthocyanins) content. The pretreatment was carried out using blanching, reduced pressure, and ultrasound (20 min, 21 kHz) in various combinations: Blanching at reduced pressure treatment conducted three times for 10 min in osmotic solution; blanching with reduced pressure for 10 min and sonicated for 20 min in osmotic solution; and blanching with 20 min of sonication and 10 min of reduced pressure. The osmotic dehydration was performed in different solutions (61.5% sucrose and 30% sucrose with the addition of 0.1% of steviol glycosides) to ensure the acceptable taste of the final product. The changes caused by the pretreatment affected the osmotic dehydration process by improving the efficiency of the process. The use of combined pretreatment led to an increase of dry matter from 9.3% to 28.4%, and soluble solids content from 21.2% to 41.5%, lightness around 17.3% to 56.9%, as well as to the reduction of bioactive compounds concentration until even 39.2% in comparison to the blanched sample not subjected to combined treatment. The osmotic dehydration caused further changes in all investigated properties.
Changes in the Microbial Community Diversity of Oil Exploitation
Authors: Liu Jingjing , Wu Jing , Lin Jiawei , Zhao Jian , Xu Tianyi , Yang Qichang , Zhao Jing , Zhao Zhongming , Song Xiaofeng
Published: July 2019

To systematically evaluate the ecological changes of an active offshore petroleum production system, the variation of microbial communities at several sites (virgin field, wellhead, storage tank) of an oil production facility in east China was investigated by sequencing the V3 to V4 regions of 16S ribosomal ribonucleic acid (rRNA) of microorganisms. In general, a decrease of microbial community richness and diversity in petroleum mining was observed, as measured by operational taxonomic unit (OTU) numbers, α (Chao1 and Shannon indices), and β (principal coordinate analysis) diversity. Microbial community structure was strongly affected by environmental factors at the phylum and genus levels. At the phylum level, virgin field and wellhead were dominated by Proteobacteria, while the storage tank had higher presence of Firmicutes (29.3–66.9%). Specifically, the wellhead displayed a lower presentence of Proteobacteria (48.6–53.4.0%) and a higher presence of Firmicutes (24.4–29.6%) than the virgin field. At the genus level, the predominant genera were Ochrobactrum and Acinetobacter in the virgin field, Lactococcus and Pseudomonas in the wellhead, and Prauseria and Bacillus in the storage tank. Our study revealed that the microbial community structure was strongly affected by the surrounding environmental factors, such as temperature, oxygen content, salinity, and pH, which could be altered because of the oil production. It was observed that the various microbiomes produced surfactants, transforming the biohazard and degrading hydro-carbon. Altering the microbiome growth condition by appropriate human intervention and taking advantage of natural microbial resources can further enhance oil recovery technology.
Increased Risk of Carotid Atherosclerosis in Male Patients with Chronic Periodontitis: A Nationwide Population-Based Retrospective Cohort Study
Authors: Tong Ching , Wang Yu-Hsun , Chang Yu-Chao
Published: July 2019

Carotid artery stenosis is a narrowing or constriction of any part of the carotid arteries, usually caused by atherosclerosis. However, no studies have specifically evaluated the association between carotid atherosclerosis (CA) and chronic periodontitis (CP). This study was to investigate the role of CP in increasing the subsequent risk of CA in the overall Taiwanese population. We carried out this retrospective cohort study, employing data derived from the National Health Insurance Research Database. A total of 72,630 patients who were newly diagnosed with CP from 2001 to 2012 were selected. For a propensity-matched control group, 72,630 healthy patients without CP were picked at random, matched according to age, sex, and index year from the general population. Cox proportional hazard regression analysis, which included sex, age, and comorbidities, was adopted to assess the hazard ratio (HR) of CA between the CP cohort and non-CP cohort. The average ages of the CP and non-CP groups were 44.02 ± 14.63 years and 44.15 ± 14.41 years, respectively. The follow up durations were 8.65 and 8.59 years for CP and non-CP groups, respectively. The results demonstrated that 305 and 284 patients with newly diagnosed CA were found in the CP cohort and non-CP cohort, respectively. There was no significant difference of developing CA in the CP cohort compared with the non-CP cohort (adjusted HR) 1.01, 95% confidence interval (CI, 0.86–1.19). However, multivariate Cox regression analysis indicated that the male group had significantly higher incidence risk of CA (log rank p = 0.046). In conclusion, this nationwide retrospective cohort study indicated that male patients with CP exhibited a significantly higher risk of CA than those without CP.
The Contribution of Geogenic Particulate Matter to Lung Disease in Indigenous Children
Authors: Shepherd Carrington C. J. , Clifford Holly D. , Mitrou Francis , Melody Shannon M. , Bennett Ellen J. , Johnston Fay H. , Knibbs Luke D. , Pereira Gavin , Pickering Janessa L. , Teo Teck H. , Kirkham Lea-Ann S. , Thornton Ruth B. , Kicic Anthony , Ling Kak-Ming , Alach Zachary , Lester Matthew , Franklin Peter , Reid David , Zosky Graeme R.
Published: July 2019

Indigenous children have much higher rates of ear and lung disease than non-Indigenous children, which may be related to exposure to high levels of geogenic (earth-derived) particulate matter (PM). The aim of this study was to assess the relationship between dust levels and health in Indigenous children in Western Australia (W.A.). Data were from a population-based sample of 1077 Indigenous children living in 66 remote communities of W.A. (>2,000,000 km2), with information on health outcomes derived from carer reports and hospitalisation records. Associations between dust levels and health outcomes were assessed by multivariate logistic regression in a multi-level framework. We assessed the effect of exposure to community sampled PM on epithelial cell (NuLi-1) responses to non-typeable Haemophilus influenzae (NTHi) in vitro. High dust levels were associated with increased odds of hospitalisation for upper (OR 1.77 95% CI [1.02–3.06]) and lower (OR 1.99 95% CI [1.08–3.68]) respiratory tract infections and ear disease (OR 3.06 95% CI [1.20–7.80]). Exposure to PM enhanced NTHi adhesion and invasion of epithelial cells and impaired IL-8 production. Exposure to geogenic PM may be contributing to the poor respiratory health of disadvantaged communities in arid environments where geogenic PM levels are high.
Preparation of Ceramsite Based on Waterworks Sludge and Its Application as Matrix in Constructed Wetlands
Authors: Wang Yaning , Yang Jinhu , Xu Hang , Liu Chenwei , Shen Zhen , Hu Kai
Published: July 2019

The recycling of waterworks sludge has become a trending issue because it not only solves the problem of difficult disposal but also saves land resources. This paper aimed to provide a new idea for the utilization of waterworks sludge to form ceramsite and to purify sewage. The specific surface area, average pore size, and pore volume of the made ceramsite were 8.15 m2/g, 8.53 nm, and 1.88 cm2/g, respectively. The made ceramsite was applied in a vertical-flow constructed wetland, and the removal efficiency of nitrogen, phosphorus and organic matter in sewage were investigated under the conditions of different start-up periods, hydraulic retention times, matrix filling heights and water quality. The removal rates of chemical oxygen demand (COD), ammonia nitrogen (NH3-N), and total phosphorus (TP) in the constructed wetlands were stable at 70%, 60%, and 79%, respectively. This constructed wetland with a ceramic matrix has certain advantages in the total amount of denitrifying microorganisms, with a proportion of 14.92%. The results prove the feasibility of preparing ceramsite from waterworks sludge and applying it as a matrix in a constructed wetland to purify sewage.
A Process-Centered Approach to the Description of Clinical Pathways—Forms and Determinants
Authors: Szelągowski Marek , Berniak-Woźny Justyna
Published: July 2019

The aim of the study is to indicate the need for variability in the presentation of clinical pathways, in various phases of their implementation, and to define the forms of presentation of clinical pathways required by physicians in both the Hospital Information Systems (HIS) and Electronic Medical Records (EMR) Systems, as well as the determinants of the selection of the forms of description, in relation to the performed medical actions. The results of the study are a significant lead-in towards further research on the required form of the user interface in systems supporting dynamic business process management (dynamic BPM). The research is a pilot of a survey study, conducted to ascertain the usefulness and feasibility of the adopted methodology, for a wider project on the determinants of the form of description of clinical pathways. An exploratory pilot survey, in a large multi-specialization hospital in Poland, was conducted. The survey sample consisted of 28 purposely selected heads of all hospital departments, and the medical team of the pediatric ward. Descriptive analysis was carried out on the data collected. The results of the study have unambiguously supported the claim that physicians require the form of presentation of clinical pathways to change, depending on the particular phase of the diagnostic–therapeutic process, as well as establishing the main determinants thereof. This pilot study is one of the first attempts to establish the factors determining the choice of clinical pathway presentation in HIS/EMR systems. While not conclusively decisive in terms of the forms of presentation or the determinants of their choice, it indicates the directions of further research, both from the point of view of ergonomics and the usability of HIS/EMR systems, as well as the management of medical knowledge, as part of the dynamic management of clinical pathways.
WIC Participation and Breastfeeding after the 2009 WIC Revision: A Propensity Score Approach
Authors: Li Kelin , Wen Ming , Reynolds Megan , Zhang Qi
Published: July 2019

In this study, we examined the association between participation in the Special Supplemental Nutrition Program for Women, Infants, and Children (WIC) and breastfeeding outcomes before and after the 2009 revisions. Four-thousand-three-hundred-and-eight WIC-eligible children younger than 60 months were included from the 2005–2014 National Health and Nutrition Examination Survey (NHANES). We compared two birth cohorts with regard to their associations between WIC participation and being ever-breastfed and breastfed at 6 months. We estimated the average effect of the treatment for the treated to assess the causal effect of WIC participation on breastfeeding based on propensity score matching. The results showed that WIC-eligible participating children born between 2000 and 2008 were significantly less likely than WIC-eligible nonparticipating children to ever receive breastfeeding (p < 0.05) or to be breastfed at 6 months (p < 0.05). Among children born between 2009 and 2014, WIC-eligible participating children were no longer less likely to ever receive breastfeeding compared to WIC-eligible nonparticipating children; the gap remained in breastfeeding at 6-months (p < 0.05). The disparities in prevalence of ever breastfed between WIC-eligible participants and nonparticipants have been eliminated since the 2009 WIC revision. More efforts are needed to improve breastfeeding persistence among WIC-participating mother–infant dyads.
Enhanced Simultaneous Nitrogen and Phosphorus Removal in A Denitrifying Biological Filter Using Waterworks Sludge Ceramsite Coupled with Iron-Carbon
Authors: Zheng Xiaoying , Jin Mengqi , Xu Hang , Chen Wei , Zhang Yuan , Yang Mengmeng , Shao Xiaoyao , Xu Zhi , Wang Weihong
Published: July 2019

In this study, waterworks sludge ceramsite (WSC) was combined with 3% iron-carbon matrix in a denitrifying biological filter (ICWSC-DNBF) to enhance the simultaneous removal of carbon, nitrogen and phosphorus in secondary effluent of wastewater treatment plant (SE-WTP). The chemical oxygen demand (COD) and nitrogen removal, as well as phosphorus removal and the adsorbed forms of phosphorus were measured and the removal mechanism of these pollutants by the ICWSC-DNBF system for treating SE-WTP were investigated. The results showed that the ICWSC-DNBF achieved good removals of COD, NH4+-N, NO3−-N, total N and total P; effluent concentrations were 17.23 mg/L, 3.72 mg/L, 14.32 mg/L, 17.38 mg/L and 0.82 mg/L, respectively. WSC enhanced the P removal due to its high specific surface area and the high number of adsorption sites. Fe-P and Al-P were the main forms of P adsorbed by WSC, accounting for 78.53% of the total adsorbed P. WSC coupled with Fe and C improved the biodegradability of SE-WTP and promoted the removal of organic matter. The removal of N was attributed to the abundant denitrifying microorganisms in the system and the electrochemical effect produced by the internal electrolysis of Fe and C.
The RND1 Small GTPase: Main Functions and Emerging Role in Oncogenesis
Authors: Mouly Laetitia , Gilhodes Julia , Lemarié Anthony , Cohen-Jonathan Moyal Elizabeth , Toulas Christine , Favre Gilles , Sordet Olivier , Monferran Sylvie
Published: July 2019

The Rho GTPase family can be classified into classic and atypical members. Classic members cycle between an inactive Guanosine DiPhosphate -bound state and an active Guanosine TriPhosphate-bound state. Atypical Rho GTPases, such as RND1, are predominantly in an active GTP-bound conformation. The role of classic members in oncogenesis has been the subject of numerous studies, while that of atypical members has been less explored. Besides the roles of RND1 in healthy tissues, recent data suggest that RND1 is involved in oncogenesis and response to cancer therapeutics. Here, we present the current knowledge on RND1 expression, subcellular localization, and functions in healthy tissues. Then, we review data showing that RND1 expression is dysregulated in tumors, the molecular mechanisms involved in this deregulation, and the role of RND1 in oncogenesis. For several aggressive tumors, RND1 presents the features of a tumor suppressor gene. In these tumors, low expression of RND1 is associated with a bad prognosis for the patients. Finally, we highlight that RND1 expression is induced by anticancer agents and modulates their response. Of note, RND1 mRNA levels in tumors could be used as a predictive marker of both patient prognosis and response to anticancer agents.
Shape-Controlled Syntheses of Magnetite Microparticles and Their Magnetorheology
Authors: Abe Hiroya , Naka Takashi , Sato Kazuyoshi , Suzuki Yoshikazu , Nakano Masami
Published: July 2019

Magnetic microspheres in a concentrated suspension can be self-assembled to form chain structures under a magnetic field, resulting in an enhanced viscosity and elasticity of the suspension (i.e., the magnetorheological (MR) effect). Recently, interest has been raised about the relationship between nonspherical particles, such as octahedral particles and the MR effect. However, experimental studies have not made much progress toward clarifying this issue due to the difficulty associated with synthesizing microparticles with well-defined shapes and sizes. Here, we presented a method for the shape-controlled synthesis of magnetite (Fe3O4) microparticles and investigated the MR effects of two suspensions prepared from the two shape-controlled samples of Fe3O4 microparticles. Our method, which was based on the polyol method, enabled the preparation of spherical and octahedral Fe3O4 microparticles with similar sizes and magnetic properties, through a reduction of α-FeOOH in a mixed solvent of ethylene glycol (a polyol) and water. The water played an important role in both the phase transition (α-FeOOH to Fe3O4) and the shape control. No substantial difference in the MR effect was observed between an octahedral-particle-based suspension and a spherical-particle-based one. Therefore, in this study, the shape of the microparticles did not strongly influence the MR effect, i.e., the properties of the chain structures.
Pathogenic Variants in STXBP1 and in Genes for GABAa Receptor Subunities Cause Atypical Rett/Rett-like Phenotypes
Authors: Cogliati Francesca , Giorgini Valentina , Masciadri Maura , Bonati Maria Teresa , Marchi Margherita , Cracco Irene , Gentilini Davide , Peron Angela , Savini Miriam Nella , Spaccini Luigina , Scelsa Barbara , Maitz Silvia , Veneselli Edvige , Prato Giulia , Pintaudi Maria , Moroni Isabella , Vignoli Aglaia , Larizza Lidia , Russo Silvia
Published: July 2019

Rett syndrome (RTT) is a neurodevelopmental disorder, affecting 1 in 10,000 girls. Intellectual disability, loss of speech and hand skills with stereotypies, seizures and ataxia are recurrent features. Stringent diagnostic criteria distinguish classical Rett, caused by a MECP2 pathogenic variant in 95% of cases, from atypical girls, 40–73% carrying MECP2 variants, and rarely CDKL5 and FOXG1 alterations. A large fraction of atypical and RTT-like patients remain without genetic cause. Next Generation Sequencing (NGS) targeted to multigene panels/Whole Exome Sequencing (WES) in 137 girls suspected for RTT led to the identification of a de novo variant in STXBP1 gene in four atypical RTT and two RTT-like girls. De novo pathogenic variants—one in GABRB2 and, for first time, one in GABRG2—were disclosed in classic and atypical RTT patients. Interestingly, the GABRG2 variant occurred at low rate percentage in blood and buccal swabs, reinforcing the relevance of mosaicism in neurological disorders. We confirm the role of STXBP1 in atypical RTT/RTT-like patients if early psychomotor delay and epilepsy before 2 years of age are observed, indicating its inclusion in the RTT diagnostic panel. Lastly, we report pathogenic variants in Gamma-aminobutyric acid-A (GABAa) receptors as a cause of atypical/classic RTT phenotype, in accordance with the deregulation of GABAergic pathway observed in MECP2 defective in vitro and in vivo models.
Intervertebral Disc Nucleus Repair: Hype or Hope?
Authors: Tendulkar Gauri , Chen Tao , Ehnert Sabrina , Kaps Hans-Peter , Nüssler Andreas K
Published: July 2019

Chronic back pain is a common disability, which is often accredited to intervertebral disc degeneration. Gold standard interventions such as spinal fusion, which are mainly designed to mechanically seal the defect, frequently fail to restore the native biomechanics. Moreover, artificial implants have limited success as a repair strategy, as they do not alter the underlying disease and fail to promote tissue integration and subsequent native biomechanics. The reported high rates of spinal fusion and artificial disc implant failure have pushed intervertebral disc degeneration research in recent years towards repair strategies. Intervertebral disc repair utilizing principles of tissue engineering should theoretically be successful, overcoming the inadequacies of artificial implants. For instance, advances in the development of scaffolds aided with cells and growth factors have opened up new possibilities for repair strategies. However, none has reached the stage of clinical trials in humans. In this review, we describe the hitches encountered in the musculoskeletal field and summarize recent advances in designing tissue-engineered constructs for promoting nucleus pulposus repair. Additionally, the review focuses on the effect of biomaterial aided with cells and growth factors on achieving effective functional reparative potency, highlighting the ways to enhance the efficacy of these treatments.
Affinity Immobilization of a Bacterial Prolidase onto Metal-Ion-Chelated Magnetic Nanoparticles for the Hydrolysis of Organophosphorus Compounds
Authors: Wang Tzu-Fan , Lo Huei-Fen , Chi Meng-Chun , Lai Kuan-Ling , Lin Min-Guan , Lin Long-Liu
Published: July 2019

In this study, silica-coated magnetic nanoparticles (SiMNPs) with isocyanatopropyltriethoxysilane as a metal-chelating ligand were prepared for the immobilization of His6-tagged Escherichia coli prolidase (His6-EcPepQ). Under one-hour coupling, the enzyme-loading capacity for the Ni2+-functionalized SiMNPs (NiNTASiMNPs) was 1.5 mg/mg support, corresponding to about 58.6% recovery of the initial activity. Native and enzyme-bound NiNTASiMNPs were subsequently characterized by transmission electron microscopy (TEM), superparamagnetic analysis, X-ray diffraction, and Fourier transform infrared (FTIR) spectroscopy. As compared to free enzyme, His6-EcPepQ@NiNTASiMNPs had significantly higher activity at 70 °C and pH ranges of 5.5 to 10, and exhibited a greater stability during a storage period of 60 days and could be recycled 20 times with approximately 80% retention of the initial activity. The immobilized enzyme was further applied in the hydrolysis of two different organophosphorus compounds, dimethyl p-nitrophenyl phosphate (methyl paraoxon) and diethyl p-nitrophenyl phosphate (ethyl paraoxon). The experimental results showed that methyl paraoxon was a preferred substrate for His6-EcPepQ and the kinetic behavior of free and immobilized enzymes towards this substance was obviously different. Taken together, the immobilization strategy surely provides an efficient means to deposit active enzymes onto NiNTASiMNPs for His6-EcPepQ-mediated biocatalysis.
Battle between Host Immune Cellular Responses and HCMV Immune Evasion
Authors: Manandhar Trishna , Hò Gia-Gia T. , Pump Wiebke C. , Blasczyk Rainer , Bade-Doeding Christina
Published: July 2019

Human cytomegalovirus (HCMV) is ubiquitously prevalent. HCMV infection is typically asymptomatic and controlled by the immune system in healthy individuals, yet HCMV can be severely pathogenic for the fetus during pregnancy and in immunocompromised persons, such as transplant recipients or HIV infected patients. HCMV has co-evolved with the hosts, developed strategies to hide from immune effector cells and to successfully survive in the human organism. One strategy for evading or delaying the immune response is maintenance of the viral genome to establish the phase of latency. Furthermore, HCMV immune evasion involves the downregulation of human leukocyte antigens (HLA)-Ia molecules to hide infected cells from T-cell recognition. HCMV expresses several proteins that are described for downregulation of the HLA class I pathway via various mechanisms. Here, we review the wide range of immune evasion mechanisms of HCMV. Understanding the mechanisms of HCMV immune evasion will contribute to the development of new customized therapeutic strategies against the virus.
Revisiting Alkali Metals As a Tool to Characterize Patterns of Mosquito Dispersal and Oviposition
Authors: Marcantonio Matteo , Winokur Olivia C. , Barker Christopher M.
Published: July 2019

Mark-recapture methods constitute a set of classical ecological tools that are used to collect information on species dispersal and population size. These methods have advanced knowledge in disparate scientific fields, from conservation biology to pest control. Gathering information on the dispersal of mosquito species, such as Aedes aegypti, has become critical since the recognition of their role as vectors of pathogens. Here, we evaluate a method to mark mosquitoes that exploits the rare alkali metals rubidium (Rb) and caesium (Cs), which have been used previously to mark adult insects through feeding. We revised this method by adding Rb and Cs directly to water in which the immature stages of Ae. aegypti were allowed to develop. We then assessed the effect of Rb- and Cs-enriched water on fitness, survival and bioaccumulation in both adult females and their eggs. Results indicated that Cs had adverse effects on Ae. aegypti, even at low concentrations, whereas Rb at low concentrations had no measured effects on exposed individuals and accumulated at detectable levels in adult females. The method described here relies on passive uptake of Rb during immature stages, which has the benefit of avoiding handling or manipulation of the dispersive adults, which enables purer measurement of movement. Moreover, we demonstrated that Rb was transferred efficiently from the marked females to their eggs. To our knowledge, Rb is the only marker used for mosquitoes that has been shown to transfer vertically from females to eggs. The application of Rb rather than more traditional markers may therefore increase the quality (no impact on released individuals) and quantity (both adults and eggs are marked) of data collected during MR studies. The method we propose here can be used in combination with other markers, such as stable isotopes, in order to maximize the information collected during MR experiments.
Addiction as Learned Behavior Patterns
Authors: Heinz Andreas , Beck Anne , Halil Melissa Gül , Pilhatsch Maximilian , Smolka Michael N. , Liu Shuyan
Published: July 2019

Individuals with substance use disorders (SUDs) have to cope with drug-related cues and contexts which can affect instrumental drug seeking, as shown with Pavlovian-to-instrumental transfer (PIT) tasks among humans and animals. Our review addresses two potential mechanisms that may contribute to habitual or even compulsive drug seeking and taking. One mechanism is represented by Pavlovian and PIT effects on drug intake. The other is a shift from goal-directed to habitual drug intake, which can be accessed via model-based versus model-free decision-making in respective learning tasks. We discuss the impact of these learning mechanisms on drug consumption. First, we describe how Pavlovian and instrumental learning mechanisms interact in drug addiction. Secondly, we address the effects of acute and chronic stress exposure on behavioral and neural PIT effects in alcohol use disorder (AUD). Thirdly, we discuss how these learning mechanisms and their respective neurobiological correlates can contribute to losing versus regaining control over drug intake. Utilizing mobile technology (mobile applications on smartphones including games that measure learning mechanisms, activity bracelets), computational models, and real-world data may help to better identify patients with a high relapse risk and to offer targeted behavioral and pharmacotherapeutic interventions for vulnerable patients.
Non-CYP2D6 Variants Selected by a GWAS Improve the Prediction of Impaired Tamoxifen Metabolism in Patients with Breast Cancer
Authors: Hennig Ewa E. , Piątkowska Magdalena , Goryca Krzysztof , Pośpiech Ewelina , Paziewska Agnieszka , Karczmarski Jakub , Kluska Anna , Brewczyńska Elżbieta , Ostrowski Jerzy
Published: July 2019

A certain minimum plasma concentration of (Z)-endoxifen is presumably required for breast cancer patients to benefit from tamoxifen therapy. In this study, we searched for DNA variants that could aid in the prediction of risk for insufficient (Z)-endoxifen exposure. A metabolic ratio (MR) corresponding to the (Z)-endoxifen efficacy threshold level was adopted as a cutoff value for a genome-wide association study comprised of 287 breast cancer patients. Multivariate regression was used to preselect variables exhibiting an independent impact on the MR and develop models to predict below-threshold MR values. In total, 15 single-nucleotide polymorphisms (SNPs) were significantly associated with below-threshold MR values. The strongest association was with rs8138080 (WBP2NL). Two alternative models for MR prediction were developed. The predictive accuracy of Model 1, including rs7245, rs6950784, rs1320308, and the CYP2D6 genotype, was considerably higher than that of the CYP2D6 genotype alone (AUC 0.879 vs 0.758). Model 2, which was developed using the same three SNPs as for Model 1 plus rs8138080, appeared as an interesting alternative to the full CYP2D6 genotype testing. In conclusion, the four novel SNPs, tested alone or in combination with the CYP2D6 genotype, improved the prediction of impaired tamoxifen-to-endoxifen metabolism, potentially allowing for treatment optimization.
Clusters of Glycemic Response to Oral Glucose Tolerance Tests Explain Multivariate Metabolic and Anthropometric Outcomes of Bariatric Surgery in Obese Patients
Authors: Szczerbinski Lukasz , Taylor Mark A. , Citko Anna , Gorska Maria , Larsen Steen , Hady Hady Razak , Kretowski Adam
Published: July 2019

Glycemic responses to bariatric surgery are highly heterogeneous among patients and defining response types remains challenging. Recently developed data-driven clustering methods have uncovered subtle pathophysiologically informative patterns among patients without diabetes. This study aimed to explain responses among patients with and without diabetes to bariatric surgery with clusters of glucose concentration during oral glucose tolerance tests (OGTTs). We assessed 30 parameters at baseline and at four subsequent follow-up visits over one year on 154 participants in the Bialystok Bariatric Surgery Study. We applied latent trajectory classification to OGTTs and multinomial regression and generalized linear mixed models to explain differential responses among clusters. OGTT trajectories created four clusters representing increasing dysglycemias that were discordant from standard diabetes diagnosis criteria. The baseline OGTT cluster increased the predictive power of regression models by over 31% and aided in correctly predicting more than 83% of diabetes remissions. Principal component analysis showed that the glucose homeostasis response primarily occurred as improved insulin sensitivity concomitant with improved the OGTT cluster. In sum, OGTT clustering explained multiple, correlated responses to metabolic surgery. The OGTT is an intuitive and easy-to-implement index of improvement that stratifies patients into response types, a vital first step in personalizing diabetic care in obese subjects.
Relationships between Multimorbidity and Suicidal Thoughts and Plans among Korean Adults
Authors: Huh Youn , Nam Ga Eun , Kim Yang-Hyun , Lee Jun Hyung
Published: July 2019

Multimorbidity and suicide rates are rising simultaneously among Korean adults. To address this issue, we assessed the association between multimorbidity and suicidal behavior among adults aged ≥19 years in Korea. We analyzed the data from the 2017 Korea National Health and Nutrition Examination Survey. Multimorbidity was defined as experiencing two or more chronic diseases. We compared the presence of suicidal thoughts and plans according to multimorbidity using chi-square test, and examined the associations between multimorbidity and suicidal thoughts and plans using multiple binary logistic regression analyses. Multimorbidity was found in 30.8% of total participants. As the number of chronic diseases increased, the percentage of thoughts and plans tended to increase (p < 0.001 and p = 0.002). Among participants with multimorbidities, 8.5% had suicidal thoughts, whereas only 3.4% without multimorbidity had such thoughts (p < 0.001). Participants with multimorbidity had significantly higher odds of suicidal thoughts (OR = 2.14; 95% CI = 1.54–2.97) and suicidal plans (OR = 2.01; 95% CI = 1.08–3.73) compared to those without multimorbidity after adjusting confounding variables. Conclusion: People with multimorbidity had a higher prevalence of suicidal thoughts and plans. Early detection of and intervention for suicidal thoughts and plans are critical for suicide prevention among people with multimorbidity.
Possibility of Fabricating Anisotropic Conductive Film with a Line-and-Space-Like Pattern by Stick-Slip Accompanying Abrasion
Authors: Naito Keishi , Kataoka Yuji , Yashiro Kisaragi
Published: July 2019

The development of an anisotropic conductive film was attempted using original microfabrication (stick-slip (SS) processing) involving SS accompanying abrasion. During SS processing, the film surface was periodically scraped with a razor. For a metal-deposited polyethylene terephthalate (PET) film (metal/PET film), fine periodic structures (line and space (L/S)-like patterns) were formed on its surface because the metal layer was periodically scraped. This result proved that SS processing can be applied not only to polymers but also to metals. Moreover, the line interval of an SS-processed metal/PET film shortened as the contact force decreased, and the depth of the line became shallower in proportion to the line interval. In addition, SS-processed copper/PET (Cu/PET) film did not conduct electricity in the direction perpendicular to the Cu line; however, it conducted electricity in the direction parallel. Thus, this study shows the possibility of using SS processing for fabricating an anisotropic conductive film.
Sustainability Assessment during Machining Ti-6Al-4V with Nano-Additives-Based Minimum Quantity Lubrication
Authors: Kishawy Hossam A. , Hegab Hussien , Deiab Ibrahim , Eltaggaz Abdelkrem
Published: July 2019

The implementation of sustainable machining process can be accomplished by different strategies including process optimization and selection of the proper lubrication techniques and cutting conditions. The present study is carried out from the perspective of a sustainability assessment of turning Ti-6Al-4V by employing minimum quantity lubrication (MQL) and MQL-nanofluid with consideration of the surface quality, tool wear, and power consumption. A sustainability assessment algorithm was used to assess the cutting processes of Ti-6Al-4V alloy under a minimum quantity of lubrication–nanofluid to estimate the levels of sustainable design variables. The assessment included the sustainable indicators as well as the machining responses in a single integrated model. The sustainable aspects included in this study were; environmental impact, management of waste, and safety and health issues of operators. The novelty here lies in employing a comprehensive sustainability assessment model to discuss and understand the machining process with MQL-nanofluid, by not only considering the machining quality characteristics, but also taking into account different sustainability indicators. In order to validate the effectiveness of the sustainability results, a comparison between the optimal and predicted responses was conducted and a good agreement was noticed. It should be stated that MQL-nanofluid showed better results compared to the cutting tests conducted under using classical MQL.
Numerical Analysis on the Effect of Artificial Ventilated Pipe Diameter on Hydrodynamic Performance of a Surface-Piercing Propeller
Authors: Gao Zeyang , Yang Dongmei , Li Ping , Dong Yue
Published: July 2019

Under the condition of large water immersion, surface-piercing propellers are inclined to be heavy loaded. In order to improve the hydrodynamic performance of the surface-piercing propeller, the installation of a vent pipe in front of a propeller disc is more widely used in the propulsion device of high speed planning crafts. Based on computational fluid dynamics (CFD) method, this paper studied the influence of diverse vent pipe diameters on hydrodynamic performance of the surface-piercing propeller under full water immersion conditions. The numerical results show that, with the increase of vent pipe diameters, the thrust and torque of the surface-piercing propeller decrease after ventilation, and the efficiency of the propeller increases rapidly; the low pressure area near the back root of the blade becomes smaller and smaller gradually; and the peak of periodic vibration of thrust and torque can be effectively reduced. The numerical results demonstrate that the installation of artificial vent pipe effectively improves the hydrodynamic performance of surface piercing propeller in the field of high speed crafts, and the increase of artificial vent pipe diameter plays an active role in the propulsion efficiency of the surface-piercing propeller.
Periorbital Reconstruction by “Periorbital Patch” Technique Using a Pericardium-Based Collagen Membrane and Titanium Mesh
Authors: Tanaskovic Nenad , Trajkovski Branko , Perić Kačarević Željka , Rider Patrick M. , Houshmand Alireza , Xiong Xin , Jung Ole , Barbeck Mike
Published: July 2019

Objective: Titanium mesh is a commonly used material for the reconstruction of orbital floor fractures. However, in some instances, a subsequent inflammatory reaction can occur that causes the adhesion of orbital tissue to the titanium mesh. The adhesion of the orbital soft tissue to the mesh causes diplopia, lid rigidity and extraocular movements restriction. This study was performed to determine if the placement of a collagen membrane over a titanium mesh can prevent the adhesion of orbital soft tissue for an improved clinical outcome. Clinical considerations: A case study was performed investigating 106 patients undergoing a periorbital restoration. Seventy-two patients received a titanium mesh without a barrier membrane, 12 patients received a barrier membrane composed of autologous auricular cartilage to provide a barrier function and 22 patients received a pericardium collagen membrane and titanium mesh. Conclusions: Titanium has been shown to generate an intense inflammatory reaction in host tissues, which can cause fibrosis to adjacent structures. Fibrosis is an essential factor in the repair of fracture sites, however this can lead to adverse effects in the orbital socket. Fibrosis can cause cicatrization and lower eyelid retraction when induced along the lower orbital rim. An improved outcome can be achieved by using a barrier between the titanium mesh and the soft tissue, such as autogenous auricular cartilage, however, only patients treated with a resorbable collagen membrane to act as a soft tissue barricade during site regeneration, prevented the fibrosis reaction and related problems from occurring.
Exploiting Suspension Plasma Spraying to Deposit Wear-Resistant Carbide Coatings
Authors: Mahade Satyapal , Narayan Karthik , Govindarajan Sivakumar , Björklund Stefan , Curry Nicholas , Joshi Shrikant
Published: July 2019

Titanium- and chromium-based carbides are attractive coating materials to impart wear resistance. Suspension plasma spraying (SPS) is a relatively new thermal spray process which has shown a facile ability to use sub-micron and nano-sized feedstock to deposit high-performance coatings. The specific novelty of this work lies in the processing of fine-sized titanium and chromium carbides (TiC and Cr3C2) in the form of aqueous suspensions to fabricate wear-resistant coatings by SPS. The resulting coatings were characterized by surface morphology, microstructure, phase constitution, and micro-hardness. The abrasive, erosive, and sliding wear performance of the SPS-processed TiC and Cr3C2 coatings was also evaluated. The results amply demonstrate that SPS is a promising route to manufacture superior wear-resistant carbide-based coatings with minimal in situ oxidation during their processing.
Photopatternable Epoxy-Based Thermosets
Authors: Giebler Michael , Radl Simone , Ules Thomas , Griesser Thomas , Schlögl Sandra
Published: July 2019

The present work provides a comparative study on the photopatterning of epoxy-based thermosets as a function of network structure and network mobility. Local switching of solubility properties by light of a defined wavelength is achieved by exploiting versatile o-nitrobenzyl ester (o-NBE) chemistry. o-NBE derivatives with terminal epoxy groups are synthetized and thermally cured with different types of cycloaliphatic anhydrides via nucleophilic ring opening reaction. By varying the structure of the anhydride, glass transition temperature (Tg) and surface hardness are adjusted over a broad range. Once the network has been formed, the photolysis of the o-NBE groups enables a well-defined degradation of the 3D network. Fourier transform infrared (FT-IR) spectroscopy studies demonstrate that cleavage rate and cleavage yield increase with rising mobility of the network, which is either facilitated by inherent network properties (Tg below room temperature) or a simultaneous heating of the thermosets above their Tg. The formation of soluble species is evidenced by sol-gel analysis, revealing that low-Tg networks are prone to secondary photoreactions at higher exposure doses, which lead to a re-crosslinking of the cleaved polymer chains. The change in solubility properties is exploited to inscribe positive tone micropatterns within the thermosets by photolithographic techniques. Contrast curves show that the resist performance of rigid networks is superior to flexible ones, with a contrast of 1.17 and a resolution of 8 µm.
Xenon Diffusion Mechanism and Xenon Bubble Nucleation and Growth Behaviors in Molybdenum via Molecular Dynamics Simulations
Authors: Zhang Wenhua , Yun Di , Liu Wenbo
Published: July 2019

The behaviors of xenon in molybdenum were studied using molecular statics and molecular dynamics simulations. The diffusion mechanism of xenon atoms was studied combining molecular dynamics, nudged elastic band, and temperature-accelerated dynamics methods. The vacancy-assisted diffusion mechanism was analyzed and the corresponding energy barriers were calculated. The clustering process of scattered xenon atoms was studied at an elevated temperature. Xenon bubbles were observed to form when the concentration of xenon atoms exceeded a threshold concentration value. Meanwhile, the interaction of xenon bubble and vacancies was investigated via the nudged elastic band method. The results showed that there exists a region around the xenon bubble where the migration energy of vacancy is significantly influenced. This work provides useful insights towards a better understanding of the behaviors of xenon in molybdenum.
Survey on the Figà–Talamanca Herz algebra
Authors: Derighetti Antoine
Published: July 2019

This paper presents a self contained approach to the theory of convolution operators on locally compact groups (both commutative and non commutative) based on the use of the Figà–Talamanca Herz algebras. The case of finite groups is also considered.