bluuebunny/crossref_metadata_2025 huggingface dataset:
Genome-wide DNA methylation profiling of CD4+ T lymphocytes identifies differentially methylated loci associated with adult primary refractory immune thrombocytopenia
Authors: Du Hanzhi , Tang Qinghua , Yang Jian , Yan Bin , Yang Lihong , Wang Mengchang
Published: June 2023

Background

DNA methylation played a crucial role in the pathogenesis of immune thrombocytopenia (ITP). However, genome-wide DNA methylation analysis has not been applied thus far. The present study aimed to provide the first DNA methylation profiling for ITP.

Methods

Peripheral blood CD4+ T lymphocytes samples were collected from 4 primary refractory ITP cases and 4 age-matched healthy controls, and DNA methylome profiling was performed using Infinium MethylationEPIC BeadChip. Differentially methylated CpG sites were further validated in another independent cohort of 10 ITP patients and 10 healthy controls using qRT-PCR.

Results

The DNA methylome profiling identified a total of 260 differentially methylated CpG sites mapping to 72 hypermethylated and 64 hypomethylated genes. These genes were mainly enriched in the actin nucleation of the Arp2/3 complex, vesicle transport, histone H3-K36 demethylation, Th1 and Th2 cell differentiation, and Notch signaling pathway according to the GO and KEGG databases. The mRNA expression of CASP9, C1orf109, and AMD1 were significantly different.

Conclusions

Given the altered DNA methylation profiling of ITP, our study provides new insights into its genetic mechanism and suggests candidate biomarkers for the diagnosis and treatment of ITP.
Protein length distribution is remarkably uniform across the tree of life
Authors: Nevers Yannis , Glover Natasha M. , Dessimoz Christophe , Lecompte Odile
Published: June 2023

Background

In every living species, the function of a protein depends on its organization of structural domains, and the length of a protein is a direct reflection of this. Because every species evolved under different evolutionary pressures, the protein length distribution, much like other genomic features, is expected to vary across species but has so far been scarcely studied.

Results

Here we evaluate this diversity by comparing protein length distribution across 2326 species (1688 bacteria, 153 archaea, and 485 eukaryotes). We find that proteins tend to be on average slightly longer in eukaryotes than in bacteria or archaea, but that the variation of length distribution across species is low, especially compared to the variation of other genomic features (genome size, number of proteins, gene length, GC content, isoelectric points of proteins). Moreover, most cases of atypical protein length distribution appear to be due to artifactual gene annotation, suggesting the actual variation of protein length distribution across species is even smaller.

Conclusions

These results open the way for developing a genome annotation quality metric based on protein length distribution to complement conventional quality measures. Overall, our findings show that protein length distribution between living species is more uniform than previously thought. Furthermore, we also provide evidence for a universal selection on protein length, yet its mechanism and fitness effect remain intriguing open questions.
Fermentation quality and bacterial community of delayed filling stylo silage in response to inoculating lactic acid bacteria strains and inoculating time
Authors: Tian Jing , Huang Liying , Tian Rong , Wu Juanyan , Tang Ruxue , Zhang Jianguo
Published: June 2023

Background

Filling silos generally takes much time in practice, which may negatively affect silage fermentation and feed intake. To clarify the effects of inoculating time, lactic acid bacteria (LAB) strains and filling time on the silage fermentation and microbes of stylo (Stylosanthes guianensis) and its silage, ensiling was quickly performed (quickly filling, QF) with or without LAB (Lactobacillus plantarum SXC48, Lb. plantarum CCZZ1 and Enterococcus faecalis XC124), and was performed after stylo inoculated with or without LAB was placed for 1 day (delayed filling, DF1) and 2 days (DF2).

Results

Delayed filling significantly increased pH, buffering capacity, microbial counts and lactic acid, acetic acid and NH3–N contents of stylo prior to ensiling. Inoculating Lb. plantarum SXC48 and CCZZ1 improved the fermentation quality of QF silage, indicated by more lactic acid, as well as lower pH and butyric acid content. Inoculating time significantly affected the pH and lactic acid content of silages. For the DF2 silages, inoculating SXC48 at filling was better than at chopping, while inoculating CCZZ1 had good fermentation quality, regardless of inoculating time. The results of 16S rRNA sequencing indicated that delayed filling enhanced the bacterial diversity of materials and silage, and inoculating significantly changed the composition of silage microbes. Kosakonia, Pseudomonas and Pantoea jointly dominated the fresh material, while Pantoea and Lelliottia jointly dominated the DF2 material. For the QF silages, inoculating SXC48 and CCZZ1 increased the relative abundance of Lactobacillus from 16.4% in the control silage to 76.5% and 82.0%, respectively. Pantoea and Lelliottia jointly dominated the DF silages. Inoculating SXC48 and CCZZ1 also increased the relative abundance of Lactobacillus in the DF stylo silages.

Conclusions

Under the present research conditions, delayed filling increased the lactic acid content and reduced the acetic acid, propionic acid and NH3–N contents of stylo silage, however, increased the bacterial diversity and relative abundance of undesirable bacteria, such as Pantoea and Lelliottia. The inoculating effectiveness varied with the LAB strains and inoculating time. Inoculating strian SXC48 at filling was better than at chopping, while inoculating strian CCZZ1 at both chopping and filling obtained the similar benefit.

Graphical Abstract
Associations between common mental disorders and menopause: cross-sectional analysis of the 2014 Adult Psychiatric Morbidity Survey
Authors: Adji Amira , Rhead Rebecca , McManus Sally , Shoham Natalie
Published: June 2023

We investigated whether women who participated in a household survey in England were more likely to screen positive for possible generalised anxiety disorder and depression during and after menopause. We used logistic regression in secondary cross-sectional analyses of 1413 participants from the 2014 Adult Psychiatric Morbidity Survey data, adjusting for potential confounders (including age, deprivation score and chronic disease).

We found that participants who were post-menopausal were more likely to screen positive for possible depression compared with participants who were pre-menopausal (3.9% v. 1.7%; adjusted odds ratio 3.91, 95% CI 1.23–12.46), but there was no association with perimenopause. We found no evidence of an association between menopausal stage and possible generalised anxiety disorder or symptom score. Clinicians should be aware of the association between menopause and depression, to best support women. Future research could focus on to what extent associations are driven by somatic features, and how this might be modified.
Current Therapeutic Targets in Cancer Cachexia: A Pathophysiologic Approach
Authors: Kadakia Kunal C. , Hamilton-Reeves Jill M. , Baracos Vickie E.
Published: June 2023

Significant progress in our understanding of cancer cachexia has occurred in recent years. Despite these advances, no pharmacologic agent has achieved US Food and Drug Administration approval for this common and highly morbid syndrome. Fortunately, improved understanding of the molecular basis of cancer cachexia has led to novel targeted approaches that are in varying stages of drug development. This article reviews two major thematic areas that are driving these pharmacologic strategies, including those targeting signal mediators at the level of the CNS and skeletal muscle. Additionally, pharmacologic strategies are being tested in combination with targeted nutrients, nutrition therapy, and exercise to treat cancer cachexia. To this end, we highlight recently published and ongoing trials evaluating cancer cachexia therapies in these specific areas.
Association of tertiary lymphoid structures with outcomes from PD-1 inhibition in advanced malignant pleural mesothelioma.
Authors: Homicsko Krisztian , Zygoura Giota , Tissot Stephanie , Popat Sanjay , Curioni-Fontecedro Alessandra , Dellaporta Tereza , O'Brien Mary , Pope Anthony , Shah Riyaz N.H. , Kammler Rosita , Finn Stephen P. , Coukos George , Peters Solange , Stahel Rolf A.
Published: June 2023

e20545

Background: Predictive biomarkers of benefit form immunotherapy in malignant mesothelioma patients remain poorly defined. The 9-15 PROMISE-MESO study (NCT02991482) of the European Thoracic Oncology Platform (ETOP) evaluated second-line pembrolizumab against chemotherapy. Pembrolizumab led to an increased response rate compared to chemotherapy, which did not translate to survival benefits. We evaluated if the presence of tertiary lymphoid structures (TLSs) with different compositions or LRRC15+ immune suppressive fibroblasts could predict the clinical benefit from pembrolizumab. Methods: We performed multiplex immune histochemistry analysis on tumor tissues. 144 patients were included in the clinical trial, from whom, 112 had available tumor tissue for analysis: 55 patients in the chemotherapy arm, and 57 patients in the immune therapy arm. We performed the following panel: calretinin or WT1 (mesothelioma cells), CD20, CD23, CD11c, LRRC15, aSMA, and DAPI. We used the Akoya Vectra Polaris platform for analysis. The IFQuant analytical pipeline developed at the Swiss Institute for Bioinformatics (SIB) was used for analyses. Results: We defined three categories of TLSs: general CD20+ TLSs independent of other markers. CD20/CD23 double-positive as mature TLSs (mTLS), and TLSs with dendritic cells (CD11c+, cTLS). Immune suppressive CAFs were defined as LRRC15+ cells/mm2. CD20+ TLSs were identified in 84% of patients' stroma, with a median of 21 TLSs/tumor tissue and 2 TLSs/mm2. mTLSs were rare, only detected in 10 of the 112 patients (9%). cTLSs with dendritic cells were more frequent and present in 45% of all patients (50/112), and roughly half of the TLS+ patients (53%) had CD11c+ cells detected in TLSs. LRRC15+ cancer-associated fibroblasts (CAFs) were detected in 85% of patients, with a median density of 61 cells/mm2. LRRC15- tumors also contained TLSs but had significantly fewer than LRRC15+ tumors (p = 0.0079). We did not find any significant association between the responses or PFS with TLS, mTLS, and LRRC15+ CAFs. We found inverse association between cTLS levels and response to immune therapy (p = 0.037) but not for PFS. Conclusions: Our data shows that advanced malignant mesotheliomas frequently harbor TLSs, but these TLSs are mostly not activated/mature. The absence of antigen-presenting cells in TLSs, like CD11c dendritic cells, is unexpectedly associated with increased response to pembrolizumab. The high prevalence of immune suppressive LRRC15+ CAFs might explain the low response rate to pembrolizumab and could be linked to the absence of maturation of TLSs. Clinical trial information: EudraCT number: 2016-002062-31 .
Maternal high fat diet multigenerationally programs HPA function and behaviors in male rat offspring
Authors: Lin ChengCheng , Lei Qiang , Yu MengNa , Lin YuHang , Lu HuiZhen , Huang YangPing , Wang Hong , Xu HuiQin , Lin YuanShao
Published: June 2023

Maternal environmental factors have been demonstrated to exert significant influences on the health of offspring. The hypothalamic-pituitary-adrenal (HPA) axis is an important neuroendocrine stress system that can be influenced by early-life challenges. Our previous research has revealed that the consumption of a high-fat diet (HFD) by pregnant and lactating rats leads to the programming of HPA axis activity in male offspring of the first generation (referred to as F1HFD/C). The present study aimed to investigate whether the observed remodeling of the HPA axis could be inherited by second-generation male offspring (referred to as F2HFD/C), following maternal HFD exposure. The results showed that F2HFD/C rats exhibited enhanced basal HPA axis activity, similar to their F1HFD/C ancestors. Moreover, F2HFD/C rats displayed exacerbated corticosterone responses to restraint and lipopolysaccharide-induced stress, but not to insulin-induced hypoglycemia stress. Furthermore, maternal HFD exposure significantly aggravated depression-like behavior in the F2 generation subjected to chronic unpredictable mild stress. To investigate the role of central CGRP signaling in maternal diet-induced programming of the HPA axis across generations, we conducted central infusion of αCGRP8-37, a CGRP receptor antagonist, in F2HFD/C rats. The results demonstrated that αCGRP8-37, attenuated depression-like behaviors and reduced the hyperresponsiveness of the HPA axis to restraint stress in these rats. Therefore, central CGRP signaling may contribute to maternal diet-induced programming of HPA axis across generations. In conclusion, our study provides evidence that maternal HFD consumption can lead to multigenerational programming of the HPA axis and behaviors in adult male descendants.
Endothelial MRs mediate Western diet-induced lipid disorders and skeletal muscle insulin resistance in females
Authors: Habibi Javad , Homan Carlton , Naz Huma , Chen Dongqing , Lastra Guido , Whaley-Connell Adam , Sowers James R , Jia Guanghong
Published: June 2023

Consumption of a Western diet (WD) consisting of excess fat and carbohydrates activates the renin-angiotensin-aldosterone system, which has emerged as an important risk factor for systemic and tissue insulin resistance. We recently discovered that activated mineralocorticoid receptors (MRs) in diet-induced obesity induces CD36 expression, increases ectopic lipid accumulation, and results in systemic and tissue insulin resistance. Here, we further investigated whether endothelial cell (EC) specific MR (ECMR) activation participates in WD-induced ectopic skeletal muscle lipid accumulation, insulin resistance and dysfunction. Six-week-old female ECMR knockout (ECMR-/-) and wild type (ECMR+/+) mice were fed either a WD or a chow diet for 16 weeks. ECMR-/- mice were found to have decreased WD-induced in vivo glucose intolerance and insulin resistance at 16-weeks. Improved insulin sensitivity was accompanied by increased glucose transporter type 4 (Glut4) expression in conjunction with improved soleus insulin metabolic signaling in phosphoinositide 3-kinases/protein kinase B and endothelial nitric oxide synthase activation. Additionally, ECMR-/- also blunted WD–induced increases in CD36 expression and associated elevations in soleus free fatty acid, total intramyocellular lipid content, oxidative stress, and soleus fibrosis. Moreover, in vitro and in vivo activation of ECMR increased EC derived exosomal CD36 that was further taken up by skeletal muscle cells, leading to increased skeletal muscle CD36 levels. These findings indicate that in the context of an obesogenic WD, enhanced ECMR signaling increases EC derived exosomal CD36 resulting in increased uptake and elevated concentrations of CD36 in skeletal muscle cells, contributing to increased lipid metabolic disorders and soleus insulin resistance.
So Typical! Philip Roth's Age Stereotypes
Authors: Wohlmann Anita
Published: June 2023

Stereotypes and clichés about older age typically evoke criticism and resistance. Rightly so, given that they simplify, overgeneralize, distort, and limit. This article treats stereotypes and clichés as forms that can indeed have powerful and harmful meanings; however, it claims that a focus on content may overlook some of the surprising and unpredictable affordances these forms can also have: stereotypes can help order experience, offer guidelines for behavior, describe relationships, and provide solace and comfort. The article uses Philip Roth's novel Everyman as a particularly rich test case in which stereotypes and clichés abound: a grumpy and “dirty” old man sees in aging nothing but decline, tragedy, and an accumulation of illnesses—associations that age scholars have challenged for decades. These stereotypes, however, also have less predictable affordances, especially when they are conspicuously repeated and amplified via literary strategies. Rather than claiming that these strategies undermine and challenge the problematic nature of age stereotypes and clichés, this article aims to broaden the repertoire of critical approaches to these forms.
Ron Silliman's Universe: Aging, Epic Poetry, and Everyday Life
Authors: Guimarães João Paulo
Published: June 2023

This essay explores Ron Silliman's Universe, a serial poetic project which, according to the publisher's description, “were [the author] to live long enough, would take him three centuries to complete.” The article specifically focuses on the significance of the book's length for a general discussion of aging, poetry and temporality. One should bear in mind that Universe is not a retrospective work of synthesis but rather a present and future-oriented project. The point of departure for the article's analysis will be the following passage from Northern Soul, the second book in the series: “Page 43 / you will read / differently if / there are 94 to the book / than if there are just / 45, What about / 523 what then / little hen.” Notorious for his massive tomes of verse, Silliman establishes an analogy between the length of his life and the length of his work that, blows up conventional chronological accounts of life. In this poem, death, instead of looming on the horizon, is internalized as the point of transition from one page to the next, the death of an event being the birth of another. Everyday life thus reveals itself in all its subtle variety, its generative evanescence paradoxically enriching the life of the poet. Silliman's epic poetry is not, that is, a matter of mastery or life extension (it makes no claims to totalization or immortality) but rather one of life enchantment, achieved through a greater bardic attunement to the granularities of everyday life.
Picturing Solidarity: Photography and Cuban Internationalism during the Vietnam War
Authors: Chase Michelle
Published: June 2023

This article studies the use of photography in the construction of solidarity between Cuba and Vietnam during the war years. It shows the way photography helped illustrate and articulate Cuba's evolving understanding of the Vietnam War as a conflict that was analogous to, and in some ways the culmination of, Cuba's own revolution. While Cuba enthusiastically embraced and disseminated photographs from Vietnam, the meanings attached to these photos were also modified to speak to domestic concerns. By focusing on the images used in two specific solidarity campaigns devoted to imprisoned activist Võ Thị Thắng and “martyr” Nguyễn Văn Trỗi, the article explores the way Cuban understandings of acceptable forms of revolutionary violence and its gendered expressions shaped the meanings attributed to these two figures.
Hong Kong in Transition: Photography and Liberation at the End of the Pacific War
Authors: Attewell Nadine
Published: June 2023

This article thinks with and against photographs taken by British military photographers in Hong Kong at the end of World War II, during the transition from Japanese back to British colonial rule. Building on Lisa Yoneyama's account of the “postwar settlements” through which “the war's meaning” was defined and contained, I situate the photographs as part of a broader British effort to reassert the legitimacy of colonial rule at a crisis point for empire by refiguring Asian liberation as an affordance, or synonym, of British (re)occupation. At the same time, I read the photographs for what they can tell us about the liberatory knowledges that Asian colonial subjects had cultivated, or might have, throughout years of war and occupation. In this way, the article meditates on the predicaments of liberation in an Asian place where the horizon of decolonization continues to be difficult to discern, focusing on the care work necessary for survival as a crucial site and practice of liberatory political imagining.
Methods of Memory: Time Travels in the Archives
Authors: Yin Sim Chi
Published: June 2023

In this article, the author critically reflects on her long project One Day We'll Understand, a multichapter research and art work that complicates the historiography of the “Malayan Emergency,” the anticolonial war in the former British colony of Malaya (1948–60)—made in the context of a growing number of art works globally dealing with the ghosts of empire. In charting the project's various parts, the author discusses how she uses art and multiple modes of archiving to conjure the specters of colonialism and traces of resistance and generate a different form of knowledge and remembrance, and how that offers us possible paths of restitution and repair.
Back to the Future: Time Traveling to 1942
Authors: Creef Elena Tajima
Published: June 2023

Japanese new media artist Masaki Fujihata's exhibition and groundbreaking accompanying public augmented reality (AR) installation “BeHere/1942: A New Lens on the Japanese American Incarceration” remind us that even after eighty years, the ghost life of World War II Japanese American internment continues to haunt us. “BeHere/1942” revisits the War Relocation Authority's photographic history of the Japanese American incarceration experience using digital technology, including AR, which allows contemporary spectators to immerse themselves in the events of “relocation day” in the spring of 1942. Fujihata provides a new way of seeing, remembering, and experiencing photographs of the Japanese American wartime past in the present—empowered with contemporary media tools that are distinctly from what he calls “our own time”: AR.
Gluing yeast peroxisomes – composition and function of membrane contact sites
Authors: Wu Fei , de Boer Rinse , van der Klei Ida J.
Published: June 2023

Membrane contact sites are defined as regions of close proximity between two membranes; this association is mediated by protein–protein and/or protein–lipid interactions. Contact sites are often involved in lipid transport, but also can perform other functions. Peroxisomal membrane contact sites have obtained little attention compared to those of other cell organelles. However, recent studies resulted in a big leap in our knowledge of the occurrence, composition and function of peroxisomal contact sites. Studies in yeast strongly contributed to this progress. In this Review, we present an overview of our current knowledge on peroxisomal membrane contact sites in various yeast species, including Hansenula polymorpha, Saccharomyces cerevisiae, Pichia pastoris and Yarrowia lipolytica. Yeast peroxisomes form contacts with almost all other cellular organelles and with the plasma membrane. The absence of a component of a yeast peroxisomal contact site complex results in a range of peroxisomal phenotypes, including metabolic and biogenesis defects and alterations in organelle number, size or position.
On Sphærozoum, Meyen. (Thalassicolla, Huxley.) Nocti-luca, and the Polycystinæ
Authors: Müller Prof.
Published: June 2023

In the ‘Annals of Nat. Hist.,’ 2 ser., vol. 8, p. 433, Mr. Huxley describes what he regarded as a new genus of zoophytes, under the name of Thalassicolla. This production, whether animal or vegetable, is found in transparent, colourless, gelatinous masses of very various forms and size ; showing no evidence of contractility nor any power of locomotion.
Notes of a microscopical examination of “ measled “and other pork
Authors: Smith William
Published: June 2023

The subject of the present paper has of late excited much attention in this locality, the trade of the port of Cork and the industry of the neighbouring counties being immediately connected with the produce and export of provisions, a main portion of which consists of cured pork.
A Structural Refinement Technique for Protein-RNA Complexes Based on a Combination of AI-based Modeling and Flexible Docking: A Study of Musashi-1 Protein
Authors: Darai Nitchakan , Hengphasatporn Kowit , Wolschann Peter , Wolfinger Michael T , Shigeta Yasuteru , Rungrotmongkol Thanyada , Harada Ryuhei
Published: June 2023

An efficient structural refinement technique for protein-RNA complexes is proposed based on a combination of AI-based modeling and flexible docking. Specifically, an enhanced sampling method called parallel cascade selection molecular dynamics (PaCS-MD) was extended to include flexible docking to construct protein-RNA complexes from those obtained by AI-based modeling (AlphaFold2). With the present technique, the conformational sampling of flexible RNA regions is accelerated by PaCS-MD, enabling one to construct plausible models for protein-RNA complexes. For demonstration, PaCS-MD constructed several protein-RNA complexes of the RNA-binding Musashi-1 (MSI1) family of proteins, which were validated by comparing a group of crucial residues for RNA-binding with experimental complexes. Our analyses suggest that PaCS-MD improves the quality of complex modeling compared to the standard protocol based on template-based modeling (Phyre2). Furthermore, PaCS-MD could also be a beneficial technique for constructing complexes of non-native RNA-binding to proteins.
Selective Conversions of CO2 into Value-Added Chemicals via Cooperative Catalysis Using Multifunctional Catalysts
Authors: Ema Tadashi
Published: June 2023

Carbon dioxide (CO2) is not only a greenhouse gas but also a renewable carbon source for organic synthesis. Here we have summarized our studies on the conversions of CO2 into value-added chemicals via cooperative catalysis using multifunctional catalysts. For example, bifunctional metalloporphyrin catalysts with quaternary ammonium halides were developed for the selective synthesis of cyclic carbonates or polycarbonates from epoxides and CO2. Bifunctional MgII and ZnII porphyrins showed high catalytic activity for the synthesis of cyclic carbonates, while bifunctional AlIII porphyrins catalyzed the copolymerization of cyclohexene oxide and CO2 to give poly(cyclohexene carbonate). The high catalytic activities resulted from the cooperative action of the central metal ion and the quaternary ammonium salts. Chiral catalysts for the kinetic resolution of terminal or internal epoxides with CO2 were also developed. In addition, macrocyclic multinuclear metal complex Zn5L3, which was synthesized by the self-assembly of Zn(OAc)2 and a BINOL derivative (L), catalyzed the reduction of CO2 with phenylsilane to produce various useful synthetic intermediates and organic compounds.
Generation of a newAdar1p150−/−mouse demonstrates isoform-specific roles in embryonic development and adult homeostasis
Authors: Liang Zhen , Goradia Ankita , Walkley Carl R. , Heraud-Farlow Jacki E.
Published: June 2023

The RNA editing enzyme adenosine deaminase acting on RNA 1 (ADAR1) is an essential regulator of the innate immune response to both cellular and viral double-stranded RNA (dsRNA). Adenosine-to-inosine (A-to-I) editing by ADAR1 modifies the sequence and structure of endogenous dsRNA and masks it from the cytoplasmic dsRNA sensor melanoma differentiation-associated protein 5 (MDA5), preventing innate immune activation. Loss-of-function mutations inADARare associated with rare autoinflammatory disorders including Aicardi–Goutières syndrome (AGS), defined by a constitutive systemic up-regulation of type I interferon (IFN). The murineAdargene encodes two protein isoforms with distinct functions: ADAR1p110 is constitutively expressed and localizes to the nucleus, whereas ADAR1p150 is primarily cytoplasmic and is inducible by IFN. Recent studies have demonstrated the critical requirement for ADAR1p150 to suppress innate immune activation by self dsRNAs. However, detailed in vivo characterization of the role of ADAR1p150 during development and in adult mice is lacking. We identified a new ADAR1p150-specific knockout mouse mutant based on a single nucleotide deletion that resulted in the loss of the ADAR1p150 protein without affecting ADAR1p110 expression. TheAdar1p150−/−died embryonically at E11.5–E12.5 accompanied by cell death in the fetal liver and an activated IFN response. Somatic loss of ADAR1p150 in adults was lethal and caused rapid hematopoietic failure, demonstrating an ongoing requirement for ADAR1p150 in vivo. The generation and characterization of this mouse model demonstrates the essential role of ADAR1p150 in vivo and provides a new tool for dissecting the functional differences between ADAR1 isoforms and their physiological contributions.
Η μετρική της ποίησης του Καβάφη
Authors: Ραζάκη Δήμητρα
Published: June 2023

Οι στόχοι της παρούσας διδακτορικής διατριβής είναι η μελέτη της μετρικής οργάνωσης του συνόλου των ποιητικών κειμένων του Κ. Π. Καβάφη (των αναγνωρισμένων από τον ίδιο τον ποιητή, δηλαδή το σώμα της επίσημης καβαφικής ποίησης, αλλά και των άλλων κατηγοριών ποιημάτων, δηλαδή των αποκηρυγμένων, των ανέκδοτων ή κρυμμένων και των ατελών ποιημάτων) και η εξέταση της εξέλιξης της στιχουργίας του με την πάροδο του χρόνου. Η ερευνητική μέθοδος που ακολουθείται περιλαμβάνει την αναλυτική περιγραφή των ποιοτικών γνωρισμάτων των διαφόρων μορφών και των μετρικορυθμικών μέσων –με βάση την τυπολογική τους κατάταξη σε ευρύτερες και ειδικότερες κατηγορίες– και την εξαγωγή αριθμητικών αποτελεσμάτων βάσει στατιστικών μετρήσεων. Η διατριβή διαρθρώνεται σε τρία μέρη. Στο πρώτο μέρος επιχειρείται μια ιστορική ανασκόπηση της πρόσληψης της μορφής των καβαφικών ποιημάτων από την εμφάνιση του ποιητή μέχρι σήμερα, γίνεται αναφορά σε θέσεις που έχει διατυπώσει ο ίδιος ο Καβάφης σε κείμενά του σχετικά με ζητήματα στιχουργικής και, με δεδομένο το γεγονός ότι στην πλειονότητα των καβαφικών ποιητικών κειμένων (και ιδίως στη μεγάλη πλειονότητα των ποιημάτων που εντάσσονται στην ώριμη φάση της ποιητικής παραγωγής του Καβάφη) ακολουθείται η μορφή του ελευθερωμένου στίχου, καταγράφονται οι βασικοί σταθμοί της πορείας της ευρωπαϊκής και της ελληνικής ποίησης από τη χρήση του αυστηρά έμμετρου στίχου έως την ελευθέρωση της ποιητικής μορφής. Στη συνέχεια, παρουσιάζονται τα βασικά γνωρίσματα της στιχουργίας τεσσάρων ποιητών που κυριάρχησαν την περίοδο ανάπτυξης του ελευθερωμένου στίχου στην Ελλάδα, δηλαδή του Κωστή Παλαμά, του Άγγελου Σικελιανού, του Κώστα Βάρναλη και του Κ. Γ. Καρυωτάκη. Επίσης, γίνεται μετρική ανάλυση συγκεκριμένων, συντεθειμένων σε ελευθερωμένο στίχο, ποιημάτων ή αποσπασμάτων από εκτενέστερες συνθέσεις των ποιητών αυτών. Στο δεύτερο μέρος παρουσιάζονται τα ευρήματα της μετρικής ανάλυσης των καβαφικών ποιημάτων. Λεπτομερέστερα, η εξέταση εστιάζεται στη μορφική οργάνωση των ποιητικών κειμένων, στα γνωρίσματα των στίχων, στην ομοιοκαταληξία, στις φωνηεντικές συναντήσεις και στους διασκελισμούς. Το τρίτο μέρος της διατριβής αποτελεί προϊόν της έρευνας που πραγματοποιήθηκε στα χειρόγραφα του αρχείου Καβάφη, προκειμένου να μελετηθούν τα αρχικά στάδια γραφής των ποιημάτων και να διερευνηθεί η ενδεχόμενη διαφοροποίηση της μετρικής κατασκευής των ποιημάτων από το αρχικό σχεδίασμά τους έως τη στιγμή που δημοσιεύτηκαν.
Quantitative modeling for the assessment of organizations' sustainable development with the aim of formulating appropriate decision - making policies
Authors: Μπούρας Δημήτριος
Published: June 2023

Η «Ατζέντα του Οργανισμού των Ηνωμένων Εθνών (ΟΗΕ) για τη Βιώσιμη Ανάπτυξη 2030» καλεί τις επιχειρήσεις να εφαρμόσουν πρωτοβουλίες, δημιουργικότητα και καινοτομίες για την αντιμετώπιση των προκλήσεων της βιώσιμης ανάπτυξης. Υπό αυτή την έννοια, η παρούσα διατριβή προτείνει ένα μοντέλο βιωσιμότητας για επιχειρηματική χρήση. Το προτεινόμενο μοντέλο θα διευκολύνει τις επιχειρήσεις στην επιλογή των κατάλληλων δεικτών Βιώσιμης Ανάπτυξης (SDG indicators), τονίζοντας πιθανά «κενά» βιωσιμότητας και ενθαρρύνοντας σχετικές βέλτιστες πρακτικές. Για την ανάπτυξη του μοντέλου βιωσιμότητας, χρησιμοποιήθηκαν βασικές οικονομικές αρχές (όπως για παράδειγμα η ελαχιστοποίηση του κόστους και η θεωρία κινδύνου), μια έρευνα βιωσιμότητας καθώς και μια στατιστική μελέτη βασισμένη σε δεδομένα της Ευρωπαϊκής Ένωσης. Ως αποτέλεσμα, διαμορφώθηκε ένα ποσοτικό μοντέλο για την αξιολόγηση της βιώσιμης ανάπτυξης στους Οργανισμούς, που αποσκοπεί στην λήψη των κατάλληλων επιχειρησιακών αποφάσεων για τη διαμόρφωση μιας ορθής βιώσιμης πολιτικής. Το μοντέλο δοκιμάστηκε στον πραγματικό κόσμο, υπό τη μορφή μελέτης περίπτωσης (case study) που πραγματοποιήθηκε στην Ελληνική Επιτροπή Ατομικής Ενέργειας.
e-Securing the EU Borders: AI in European Integrated Border Management
Authors: Andreou Angelanna
Published: June 2023

In the European Union, the external borders were not always conceived as European ones. However, after experiencing a range of threats, the European policymakers identified external borders' importance to European security. Not forgetting that borders are connected with national authority, the EU through Frontex has made progressive steps towards a coordinated or even common border management. The main tools to achieve that are the shared European IT systems. Their enhanced capabilities, mostly based on Artificial Intelligence, will further establish the European Smart Borders. There is a complex "network” of IT border systems already operating or prospect automated ones that are necessary to be resilient and align with core fundamental rights.
Η εξέλιξη της ομαδικής και ατομικής επιχειρηματολογίας, καθώς και της γνώσης περιεχομένου των φοιτητών/τριών του Π.Τ.Δ.Ε. (ΕΚΠΑ) σε προβλήματα Νευτώνειας Μηχανικής
Authors: Τσιφτσής Γεώργιος , Χαλκιά Κρυσταλλία
Published: June 2023

Η παρούσα έρευνα εξετάζει την εξέλιξη της ατομικής και ομαδικής επιχειρηματολογίας των φοιτητών/τριών του Π.Τ.Δ.Ε. (ΕΚΠΑ). Το δείγμα αποτελούνταν από δεκαεπτά προπτυχιακούς φοιτητές/τριες, κατανεμημένους/ες σε τέσσερις ομάδες. Η έρευνα βασίστηκε σε ομαδικές συζητήσεις των φοιτητών/τριών σε πέντε προβλήματα Νευτώνειας Μηχανικής εναλλασσόμενες με τις παρεμβάσεις του ερευνητή και πραγματοποιήθηκε μέσω Skype λόγω της πανδημίας Covid-19. Οι συζητήσεις βιντεοσκοπήθηκαν και η προφορική επιχειρηματολογία αναλύθηκε με βάση το μοντέλο των Clark και Sampson. Tα γραπτά επιχειρήματα πριν και μετά την παρέμβαση (pre-και post-tests) αξιολογήθηκαν με βάση το μοντέλο των McNeill και Krajcik. Τα αποτελέσματα της ομαδικής επιχειρηματολογίας έδειξαν συνεχή βελτίωση στη μία ομάδα και αυξομειώσεις στις άλλες τρεις ομάδες, ενώ της ατομικής επιχειρηματολογίας βελτίωση των περισσότερων φοιτητών/τριών μεταξύ πρώτου και πέμπτου προβλήματος, αλλά με διακυμάνσεις μεταξύ των ενδιάμεσων προβλημάτων. Τα αποτελέσματα των pre-και post-tests έδειξαν στατιστικά σημαντική βελτίωση στις δεξιότητες επιχειρηματολογίας των φοιτητών/τριών και στην εννοιολογική κατανόηση των τριών από τα πέντε προβλήματα.
Implementing Carrier Aggregation on 4G Long Term Evolution-Advanced Network in a Dense Urban Area: A Techno-Economic Assessment
Authors: Imam Nashiruddin Muhammad , Aulia Fadhila Pinasthika , Mufti Adriansyah Nachwan , Adam Nugraha Muhammad , Rahmawati Putri
Published: June 2023

The implementation of mobile cellular networks of the fourth generation, known as 4G Long Term Evolution-Advanced (4G LTE-A), is accelerating throughout the globe. Thus, the number of users in any given area is bound to rise with technological advancements. However, internet connec-tivity has become challenging in Indonesia, particularly in the dense urban area of Central Jakarta, due to network conges-tion caused by the region’s enormous population of users and limited existing frequency. Therefore, this research aims to utilize the 2100 MHz frequency band as an additional fre-quency for the 4G LTE-A network in the dense urban Cen-tral Jakarta region. Furthermore, this research employs a techno-economic assessment of the deployment of 4G LTE-A with and without carrier aggregation (CA). The purpose is to see if expanding the 4G LTE-A network in the study area by adding the 2100 MHz frequency and utilizing the CA method is feasible and beneficial. According to the results, using the CA technique leads to fewer eNodeB requirements in the dense urban area of Central Jakarta than without the CA technique. Furthermore, through network simulation soft-ware, Forsk Atoll, good quality of the Key Performance Indi-cators (KPI) is obtained. In addition, based on the business perspective, this research acquired good Net Present Value (NPV), and the Internal Rate of Return (IRR) values are ob-tained, with returns on capital in the range of two to three years. Overall, these findings indicate that deploying 4G LTE-A technology in the planning region using 2100 MHz as an additional frequency with the CA technique is feasible and beneficial for cellular operators.
generic approach to evaluating territorial attractiveness in a bipolar cities system: the case of north-eastern Algeria
Authors: Aibeche Roufeida , Bousmaha Ahmed , Arce Ruiz Rosa Maria
Published: June 2023

Evaluating territorial attractiveness can result in several interpretations due to the multidimensional nature of the concept, starting from economy and expanding into many fi elds such as geography and social studies, in this paper, we attempt to analyze the existing economic-urban dilemma in the north-east region of Algeria by evaluating the attractiveness of its two national metropolises as separate territorial units. Th e main objective of the study was to explore the possibility of evaluating the three facets of attractiveness simultaneously in a bipolar cities system. Our method is not in itself new; it has been used as a model for assessment in many other regions of the developing world suff ering from lack of data and economic-urban issues, but this is the fi rst time it has been used to assess attractiveness. Th is multi-factorial model with synthetic indicators will help us to have a general view of the issue and to defi ne strengths and weaknesses of both territorial units. However, the limits of this methodology will be the percentage uncertainty that lies in the subjectivity of the weighting step performed by experts.
Wprowadzenie
Authors: Majewska-Kafarowska Agnieszka
Published: June 2023

W prezentowanym numerze 2/2022 Edukacji Dorosłych proponujemy Czytelnikom/Czytelniczkom teksty Autorów/Autorek z różnych ośrodków w kraju i za granicą, którzy swoje narracje andragogiczne poświęcili zagadnieniom rozmaicie wpisującym się w aktualia problemów doświadczanych przez współczesnych do- rosłych. Tematyka prezentowanych artykułów – choć nie zawsze bezpośrednio – dotyczy specyfiki ponowoczesnej rzeczywistości naznaczonej nie tylko globalizacją, technologizacją życia codziennego, ale wciąż jeszcze postpandemicznymi problemami determinującymi gospodarki wielu krajów, a zatem życie ludzi. Niestety ta rzeczywistość już niemal od roku ma jeszcze jedną odsłonę, niechlubną, trudną, obnażającą niemoc politycznych mechanizmów wpływu i dosadnie ukazującą specyfikę ludzkiego losu, zawirowań życia. Co prawda wojna nie jest obecna jako główny wątek w prezentowanych artykułach, pojawiają się natomiast takie tematy jak samotność, kształcenie całożyciowe, wypalenie akademickie, kształcenie opiekuna osoby starszej, społeczeństwo wiedzy czy edukacja seniorów.
SpołeczeńStwo wiedzy – nieobecna kategoria andragogiki
Authors: Malewski Mieczysław
Published: June 2023

W artykule wskazuje się, że wraz z cywilizacyjnym rozwojem spo- łeczeństwa zmieniały się jego epistemologiczne fundamenty. Tworzyły je kolejno: mądrość, wiedza i informacja. Autor analizuje proces przejścia od społeczeństwa wiedzy do społeczeństwa informacyjnego i zastanawia się nad skutkami, jakie zwrot ten ma dla edukacji dorosłych.
Kształcenie całożyciowe w perspektywie Europejskiego programu na rzecz umiejętności
Authors: Kula Ewa , Pękowska Marzena
Published: June 2023

W ostatnich latach znaczenia nabiera idea kształcenia cało- życiowego. Dynamicznie zmieniający się rynek pracy, wyzwania związane z rosnącym wykorzystaniem technologii i automatyzacją pracy mają wpływ na zatrudnienie oraz sektory gospodarki. Dlatego jednym z priorytetów edukacji ustawicznej jest zwiększenie udziału osób dorosłych w kształceniu i szkoleniu. Niniejsze opracowanie prezentuje nowy program na rzecz umiejętności ogłoszony przez Komisję Europejską w 2020 roku. Europejski program na rzecz umiejętności, służący zrównoważonej konkurencyjności, sprawiedliwości społecznej i odporności jest odpowiedzią na potrzebę rozwijania przez dorosłych nowych umiejętności potrzebnych w świecie coraz szybciej zachodzących zmian. W artykule dokonano analizy problematyki dotyczącej dynamiki ewolucji znaczenia idei uczenia się przez całe życie. Podjęto także rozważania na temat polityki edukacyjnej w kontekście realizowania jej koncepcji w praktyce oświatowej.
Determinación social del consumo de frutas y verduras en Florida (Valle del Cauca, Colombia, 2018)
Authors: Loaiza-Buitrago Diana Fernanda , Tombé-Reyes Shirley , Estrada-Trujillo Victoria Eugenia
Published: June 2023

Introducción: el consumo de frutas y verduras como proceso protector de la salud se ha analizado principalmente desde la perspectiva de los estilos de vida individuales; sin embargo, procesos de tipo estructural como los modelos de producción de alimentos y las políticas de alimentación determinan su producción, disponibilidad y acceso. Se planteó como objetivo analizar los procesos de determinación social del consumo de frutas y verduras en el municipio de Florida (Valle del Cauca, Colombia). Materiales y métodos: estudio cualitativo, tipo estudio de caso, con enfoque de determinación social. Incluyó revisión documental de políticas alimentarias y grupos de discusión con actores clave; el análisis de contenido permitió explicitar mensajes y discursos. Resultados: en la dimensión general, resaltan como procesos críticos de determinación, que limitan la producción y el acceso de frutas y verduras, el uso y tenencia inequitativa de la tierra y la explotación de las fuentes hídricas, y unas políticas que desde la lógica de libre mercado reducen cada vez más las estrategias locales de autosostenimiento y autoconsumo. En la dimensión particular, el conflicto armado (como proceso crítico adverso) y los ejercicios de soberanía alimentaria (como proceso crítico favorable al consumo de frutas y verduras). Conclusiones: la política alimentaria nacional y local reconoce, de manera discursiva, las determinaciones sociales de la seguridad alimentaria, pero en la práctica privilegia estrategias focalizadas y contingentes que no apuntan a la solución de condiciones estructurales que limitan la producción, disponibilidad, acceso y, por ende, el consumo de frutas y verduras.
Taking a novel approach to educating staff on infection prevention
Authors: Griffiths Amy , Fitzgibbon Pasqua
Published: June 2023

Amy Griffiths and Pasqua Fitzgibbon, Harm Free Care Nurse Specialists, Newcastle upon Tyne Hospitals NHS Foundation Trust ( amy.griffiths15@nhs.net ), achieved a Bronze award in the Infection Prevention Nurse of the Year category of the BJN Awards 2023
Body modification and the limits to consent
Authors: Griffith Richard
Published: June 2023

Richard Griffith, Senior Lecturer in Health Law at Swansea University, considers the limits to personal autonomy using body modification to illustrate the legal principles of consent
Reseña de 'Feminismo anticarcelario: el cuerpo como resistencia'
Authors: Stroppa Rachele
Published: June 2023

Reseña del libro Feminismo anticarcelario: el cuerpo como resistencia, Alicia Alonso Merino, Editorial Zambra Baladre 2023, 192 pp
Sobre la teoría materialista del castigo: Una réplica a la 'tesis de la heterogeneidad interpretativa'
Authors: Gatti Carlo , Jiménez Franco Daniel
Published: June 2023

El objetivo del presente trabajo es someter a un juicio crítico una tendencia recurrente – aquí denominada ‘tesis de la multiplicidad interpretativa’ – acerca de las teorías del castigo surgidas en el marco de la tradición marxista. Tomando a David Garland como figura más influyente de dicha postura, la confutación aquí propuesta no apunta a articular una argumentación tan abarcadora como aquella criticada. Menos ambiciosamente, lo que busca es cuestionar la presunta contraposición epistémica entre enfoques marxistas sobre el castigo, ejemplificada por Garland en la yuxtaposición entre la teoría de Pašukanis, por un lado, y aquella de Rusche y Kirchheimer, por el otro. Llevando a cabo una relectura de estos autores, y dejando de lado disputas teóricas que exceden el alcance de este trabajo, se quiere aquí plantear que cualquier aserción sobre una multiplicidad de teorías marxistas del castigo no debería basarse en datos puramente exteriores, sino en la demostrada imposibilidad de recomposiciones a través de denominadores comunes y constitutivos de la tradición teórica de referencia. La conclusión de la relectura aquí propuesta es, más bien, la existencia de una complementariedad epistemológica entre las dos teorías, no sólo por su mera compatibilidad, sino por una coincidencia de categorías de análisis y paralelismos argumentativos.
Nota Editorial, Nº 24 (2023)
Authors: Rivera Beiras Iñaki
Published: June 2023

Nota Editorial número 24 (2023). Contenidos del número y agradecimientos
García Avilés, Alejandro, Imágenes encantadas. Los poderes de la imagen en la Edad Media, Vitoria-Gasteiz, Ediciones Sans Soleil, 2021
Authors: Ferrer Ventosa Roger
Published: June 2023

Ressenya del llibre: García Avilés, Alejandro, Imágenes encantadas. Los poderes de la imagen en la Edad Media, Vitoria-Gasteiz, Ediciones Sans Soleil, 2021.
Maria Josep Ragué-Arias entre els personatges femenins de la tragèdia grega
Authors: Molins Casaña Manuel
Published: June 2023

Homenatge a Maria Josep Ragué-Arias.
Actuacions renaixentistes i neogòtiques a la façana de l'església gòtica de Santa Maria de Vilafranca del Penedès
Authors: Dorado Ladera Esther
Published: June 2023

L’objecte d’aquest article és l’estudi de la façana de l’església de Santa Maria de Vilafranca del Penedès, des de l’obra gòtica fins als nostres dies. El temple va començar a construir-se a finals del segle XIII i es va consagrar l’any 1484 amb una portalada gòtica inacabada. Al segle XVI la façana es va completar amb una galeria renaixentista que tres-cents anys després es percebia com un afegit greco-romà pagà i va suscitar crítiques importants des de tots els sectors de la societat. Aquesta situació va concloure amb l’aixecament d’una façana neogòtica projectada per August Font, que va haver de restaurar-se a mitjans de segle i que encara pot contemplar-se a l’actualitat.
De la vida escénica a la vida pública, una genealogía de la experiencia social en el siglo XVIII
Authors: Espejo Paredes José Dionisio
Published: June 2023

La cultura ilustrada, donde la división estamental muta en una sociedad de clases, va a desarrollar nuevos mecanismos de clasificación y jerarquización. El fundamento de esa nueva organización clásica procede del paradigma barroco del Theatrum Mundi, convertido, en los discursos sobre arte y teatro de los enciclopedistas, en el modelo de la esfera pública. Así lo encontramos en Diderot: el comediante es el hombre público regido por ideas y no por sentimientos. Por otra parte, la vida privada se somete, en los márgenes que permite la individualidad burguesa, a una nueva moralidad centrada en la exportación del modelo económico a lo político. Se anuncia en el modelo escénico una nueva moralidad y una nueva clasificación social resumida en “finjo luego soy”, lo real es la representación.
Sobre arte, imagen y cultura en la Edad Media
Authors: Alcoy i Pedrós Rosa
Published: June 2023

Reseña del libro: Lucía Lahoz, La imagen y su contexto cultural. La iconografía medieval, Madrid, Editorial Síntesis, 2022.