bluuebunny/crossref_metadata_2025 huggingface dataset:
From Acellular Matrices to Smart Polymers: Degradable Scaffolds that are Transforming the Shape of Urethral Tissue Engineering
Authors: Abbas Tariq O. , Yalcin Huseyin C. , Pennisi Cristian P.
Published: April 2019

Several congenital and acquired conditions may result in severe narrowing of the urethra in men, which represent an ongoing surgical challenge and a significant burden on both health and quality of life. In the field of urethral reconstruction, tissue engineering has emerged as a promising alternative to overcome some of the limitations associated with autologous tissue grafts. In this direction, preclinical as well as clinical studies, have shown that degradable scaffolds are able to restore the normal urethral architecture, supporting neo-vascularization and stratification of the tissue. While a wide variety of degradable biomaterials are under scrutiny, such as decellularized matrices, natural, and synthetic polymers, the search for scaffold materials that could fulfill the clinical performance requirements continues. In this article, we discuss the design requirements of the scaffold that appear to be crucial to better resemble the structural, physical, and biological properties of the native urethra and are expected to support an adequate recovery of the urethral function. In this context, we review the biological performance of the degradable polymers currently applied for urethral reconstruction and outline the perspectives on novel functional polymers, which could find application in the design of customized urethral constructs.
ytiB and ythA Genes Reduce the Uranium Removal Capacity of Bacillus atrophaeus
Authors: Wang Li , Xiao Shiqi , Chen Xiaoming , Chen Shilin , Wang Shanqiang , Wang Chao , Tang Yunlai , Dong Faqin
Published: April 2019

Two Bacillus atrophaeus strains, the first being a highly stress-resistant ATCC 9372 strain and the Ua strain identified from a chromium mine by our lab, differ in their abilities to tolerate and remove Uranium (VI) from contaminated water. An increase in U(VI) concentration in growth media led to a decrease in the tolerance and bio-remedial capacity of both strains. However, under high concentrations of U(VI) in the growth media, the ATCC 9372 strain demonstrated a higher tolerance and a higher removal capacity than the Ua strain. Two approaches, transcriptome sequencing and transgenic technology, were used to elucidate the relationship between particular genes within these two strains and their U(VI) removal capacity. Sequencing confirmed the expression of two genes unique to the Ua strain, previously designated ytiB and ythA. They encode putative proteins that show the highest levels of identity to carbonic anhydrase and cytochrome bd terminal oxidase I, respectively. Using the pBE-S DNA vector, ytiB and ythA were transformed into the ATCC 9372 strain of Bacillus atrophaeus. Under a U(VI) concentration of 120 mg/L, the removal rates of the transgenic ATCC 9372-ytiB and ATCC 9372-ythA strains decreased by 7.55% and 7.43%, respectively, compared to the removal rate of the control strain transformed with empty plasmid. The results suggest that both ythA and ytiB genes have a negative influence on the uranium removing capacity of Bacillus atrophaeus. This finding will help to elucidate the molecular mechanisms of uranium removal by bacteria.
Microbes in Cahoots with Plants: MIST to Hit the Jackpot of Agricultural Productivity during Drought
Authors: Kaushal Manoj
Published: April 2019

Drought conditions marked by water deficit impede plant growth thus causing recurrent decline in agricultural productivity. Presently, research efforts are focussed towards harnessing the potential of microbes to enhance crop production during drought. Microbial communities, such as arbuscular mycorrhizal fungi (AMF) and plant growth-promoting rhizobacteria (PGPR) buddy up with plants to boost crop productivity during drought via microbial induced systemic tolerance (MIST). The present review summarizes MIST mechanisms during drought comprised of modulation in phytohormonal profiles, sturdy antioxidant defence, osmotic grapnel, bacterial exopolysaccharides (EPS) or AMF glomalin production, volatile organic compounds (VOCs), expression of fungal aquaporins and stress responsive genes, which alters various physiological processes such as hydraulic conductance, transpiration rate, stomatal conductivity and photosynthesis in host plants. Molecular studies have revealed microbial induced differential expression of various genes such as ERD15 (Early Response to Dehydration 15), RAB18 (ABA-responsive gene) in Arabidopsis, COX1 (regulates energy and carbohydrate metabolism), PKDP (protein kinase), AP2-EREBP (stress responsive pathway), Hsp20, bZIP1 and COC1 (chaperones in ABA signalling) in Pseudomonas fluorescens treated rice, LbKT1, LbSKOR (encoding potassium channels) in Lycium, PtYUC3 and PtYUC8 (IAA biosynthesis) in AMF inoculated Poncirus, ADC, AIH, CPA, SPDS, SPMS and SAMDC (polyamine biosynthesis) in PGPR inoculated Arabidopsis, 14-3-3 genes (TFT1-TFT12 genes in ABA signalling pathways) in AMF treated Solanum, ACO, ACS (ethylene biosynthesis), jasmonate MYC2 gene in chick pea, PR1 (SA regulated gene), pdf1.2 (JA marker genes) and VSP1 (ethylene-response gene) in Pseudomonas treated Arabidopsis plants. Moreover, the key role of miRNAs in MIST has also been recorded in Pseudomonas putida RA treated chick pea plants.
Rhododendrin-Induced RNF146 Expression via Estrogen Receptor β Activation is Cytoprotective Against 6-OHDA-Induced Oxidative Stress
Authors: Kim Hyojung , Park Jisoo , Leem HyunHee , Cho MyoungLae , Yoon Jin-Ha , Maeng Han-Joo , Lee Yunjong
Published: April 2019

Ring finger protein 146 (RNF146) is an E3 ubiquitin ligase whose activity prevents poly (ADP-ribose) polymerase 1 (PARP1)-dependent neurodegeneration in Parkinson’s disease (PD). Previously, we reported that rhododendrin is a chemical inducer that increases RNF146 expression. However, the molecular mechanism of rhododendrin-induced RNF146 expression is largely unknown and its translational application for the treatment of Parkinson’s disease remains unexplored. Here we found that rhododendrin increased RNF146 expression via estrogen receptor β (ERβ) activation. Rhododendrin stimulated ERβ nuclear translocation and binding to the RNF146 promoter, thereby enhancing its transcription. Rhododendrin is cytoprotective against 6-hydroxydopamine (6-OHDA)-induced cell death, which is largely dependent on ERβ activity and RNF146 expression. Finally, we demonstrated that rhododendrin treatment resulted in RNF146 expression in dopaminergic neurons in mice. Moreover, dopaminergic neuron viability was markedly enhanced by pretreatment with rhododendrin in 6-OHDA-induced mouse models for PD. Our findings indicate that estrogen receptor activation plays a neuroprotective role and that rhododendrin could be a potential therapeutic agent in preventing PARP1-dependent dopaminergic cell loss in PD.
Allelic Diversity of Acetyl Coenzyme A Carboxylase accD/bccp Genes Implicated in Nuclear-Cytoplasmic Conflict in the Wild and Domesticated Pea (Pisum sp.)
Authors: Nováková Eliška , Zablatzká Lenka , Brus Jan , Nesrstová Viktorie , Hanáček Pavel , Kalendar Ruslan , Cvrčková Fatima , Majeský Ľuboš , Smýkal Petr
Published: April 2019

Reproductive isolation is an important component of species differentiation. The plastid accD gene coding for the acetyl-CoA carboxylase subunit and the nuclear bccp gene coding for the biotin carboxyl carrier protein were identified as candidate genes governing nuclear-cytoplasmic incompatibility in peas. We examined the allelic diversity in a set of 195 geographically diverse samples of both cultivated (Pisum sativum, P. abyssinicum) and wild (P. fulvum and P. elatius) peas. Based on deduced protein sequences, we identified 34 accD and 31 bccp alleles that are partially geographically and genetically structured. The accD is highly variable due to insertions of tandem repeats. P. fulvum and P. abyssinicum have unique alleles and combinations of both genes. On the other hand, partial overlap was observed between P. sativum and P. elatius. Mapping of protein sequence polymorphisms to 3D structures revealed that most of the repeat and indel polymorphisms map to sequence regions that could not be modeled, consistent with this part of the protein being less constrained by requirements for precise folding than the enzymatically active domains. The results of this study are important not only from an evolutionary point of view but are also relevant for pea breeding when using more distant wild relatives.
Characterization of the Chloroplast Genome of Trentepohlia odorata (Trentepohliales, Chlorophyta), and Discussion of its Taxonomy
Authors: Zhu Huan , Hu Yuxin , Liu Feng , Hu Zhengyu , Liu Guoxiang
Published: April 2019

Trentepohliales is an aerial order of Chlorophyta with approximately 80 species distributed mainly in tropical and subtropical regions. The taxonomy of this genus is quite difficult and presents a challenge for many phycologists. Although plentiful molecular data is available, most of the sequences are not identified at the species level. In the present study, we described a new specimen with detailed morphological data and identified it as Trentepohlia odorata. A phylogenetic analysis showed T. odorata as a novel lineage in Trentepohliales. T. odorata has the closest relationship with T. annulata, which is expected since sporangia of both species are without stalk cell and with dorsal pore. Species with such morphological characteristics may represent deep lineages in Trentepohliales. Although an increasing number of chloroplast genomes of Ulvophyceae have been reported in recent years, the whole plastome of Trentepohliales has not yet been reported. Thus, the chloroplast genome of Trentepohlia odorata was reported in the present study. The whole plastome was 399,372 bp in length, with 63 predicted protein-coding genes, 31 tRNAs, and 3 rRNAs. Additionally, we annotated 95 free-standing open reading frames, of which seven were annotated with plastid origins, 16 with eukaryotic genome origins, and 33 with bacterial genome origins. Four rpo genes (rpoA, rpoB, rpoC1, and rpoC2) were annotated within ORF clusters. These four genes were fragmented into several (partial) ORFs by in-frame stop codons. Additionally, we detected a frame shift mutation in the rpoB gene. The phylogenetic analysis supported that Trentepohliales clustered with Dasycladales and nested into the BDT clade (Bryopsidales, Dasycladales and Trentepohliales). Our results present the first whole chloroplast genome of a species of Trentepohliales and provided new data for understanding the evolution of the chloroplast genome in Ulvophyceae.
Targeting Chemoresistant Tumors: Could TRIM Proteins-p53 Axis Be a Possible Answer?
Authors: Valletti Alessio , Marzano Flaviana , Pesole Graziano , Sbisà Elisabetta , Tullo Apollonia
Published: April 2019

Chemosensitivity is a crucial feature for all tumours so that they can be successfully treated, but the huge heterogeneity of these diseases, to be intended both inter- and intra-tumour, makes it a hard-to-win battle. Indeed, this genotypic and phenotypic variety, together with the adaptability of tumours, results in a plethora of chemoresistance acquisition mechanisms strongly affecting the effectiveness of treatments at different levels. Tripartite motif (TRIM) proteins are shown to be involved in some of these mechanisms thanks to their E3-ubiquitin ligase activity, but also to other activities they can exert in several cellular pathways. Undoubtedly, the ability to regulate the stability and activity of the p53 tumour suppressor protein, shared by many of the TRIMs, represents the preeminent link between this protein family and chemoresistance. Indeed, they can modulate p53 degradation, localization and subset of transactivated target genes, shifting the cellular response towards a cytoprotective or cytotoxic reaction to whatever damage induced by therapy, sometimes in a cellular-dependent way. The involvement in other chemoresistance acquisition mechanisms, independent by p53, is known, affecting pivotal processes like PI3K/Akt/NF-κB signalling transduction or Wnt/beta catenin pathway, to name a few. Hence, the inhibition or the enhancement of TRIM proteins functionality could be worth investigating to better understand chemoresistance and as a strategy to increase effectiveness of anticancer therapies.
Extracellular Proteolytic Activity and Amino Acid Production by Lactic Acid Bacteria Isolated from Malaysian Foods
Authors: Toe Cui Jin , Foo Hooi Ling , Loh Teck Chwen , Mohamad Rosfarizan , Abdul Rahim Raha , Idrus Zulkifli
Published: April 2019

Amino acids (AAs) are vital elements for growth, reproduction, and maintenance of organisms. Current technology uses genetically engineered microorganisms for AAs production, which has urged the search for a safer food-grade AA producer strain. The extracellular proteolytic activities of lactic acid bacteria (LAB) can be a vital tool to hydrolyze extracellular protein molecules into free AAs, thereby exhibiting great potential for functional AA production. In this study, eight LAB isolated from Malaysian foods were determined for their extracellular proteolytic activities and their capability of producing AAs. All studied LAB exhibited versatile extracellular proteolytic activities from acidic to alkaline pH conditions. In comparison, Pediococcus pentosaceus UP-2 exhibited the highest ability to produce 15 AAs extracellularly, including aspartate, lysine, methionine, threonine, isoleucine, glutamate, proline, alanine, valine, leucine, tryptophan, tyrosine, serine, glycine, and cystine, followed by Pediococcus pentosaceus UL-2, Pediococcus acidilactici UB-6, and Pediococcus acidilactici UP-1 with 11 to 12 different AAs production detected extracellularly. Pediococcus pentosaceus UL-6 demonstrated the highest increment of proline production at 24 h of incubation. However, Pediococcus acidilactici UL-3 and Lactobacillus plantarum I-UL4 exhibited the greatest requirement for AA. The results of this study showed that different LAB possess different extracellular proteolytic activities and potentials as extracellular AA producers.
Toxic Effects of Low-Level Long-Term Inhalation Exposures of Rats to Nickel Oxide Nanoparticles
Authors: Sutunkova Marina P. , Solovyeva Svetlana N. , Minigalieva Ilzira A. , Gurvich Vladimir B. , Valamina Irene E. , Makeyev Oleg H. , Shur Vladimir Ya. , Shishkina Ekaterina V. , Zubarev Ilya V. , Saatkhudinova Renata R. , Klinova Svetlana V. , Tsaregorodtseva Anastasia E. , Korotkov Artem V. , Shuman Eugene A. , Privalova Larisa I. , Katsnelson Boris A.
Published: April 2019

Rats were exposed to nickel oxide nanoparticles (NiO-NP) inhalation at 0.23 ± 0.01 mg/m3 for 4 h a day 5 times a week for up to 10 months. The rat organism responded to this impact with changes in cytological and some biochemical characteristics of the bronchoalveolar lavage fluid along with a paradoxically little pronounced pulmonary pathology associated with a rather low chronic retention of nanoparticles in the lungs. There were various manifestations of systemic toxicity, including damage to the liver and kidneys; a likely allergic syndrome as indicated by some cytological signs; transient stimulation of erythropoiesis; and penetration of nickel into the brain from the nasal mucous membrane along the olfactory pathway. Against a picture of mild to moderate chronic toxicity of nickel, its in vivo genotoxic effect assessed by the degree of DNA fragmentation in nucleated blood cells (the RAPD test) was pronounced, tending to increasing with the length of the exposure period. When rats were given orally, in parallel with the toxic exposure, a set of innocuous substances with differing mechanisms of expected bioprotective action, the genotoxic effect of NiO-NPs was found to be substantially attenuated.
Human β-Defensin 2 Expression in Oral Epithelium: Potential Therapeutic Targets in Oral Lichen Planus
Authors: Salem Abdelhakim , Almahmoudi Rabeia , Hagström Jaana , Stark Holger , Nordström Dan , Salo Tuula , Eklund Kari K.
Published: April 2019

Human β-defensin 2 (hBD-2) is a potent antimicrobial peptide that participates in defense against invading bacteria. We recently showed that bacterial components and histamine, through histamine H4 receptor (H4R), are involved in the pathogenesis of the potentially malignant lesion, oral lichen planus (OLP). However, the underlying mechanisms remain unknown. We, therefore, investigated the role of hBD2–histamine crosstalk signaling in promoting OLP pathology. Biopsies from OLP and oral tongue squamous cell carcinoma (OTSCC) patients, and healthy controls were used. Two OTSCC cell lines and normal human oral keratinocytes (HOKs) were used. HBD-2 and other targets were mapped by immunostaining and analyzed by ImageJ2 software. The highly sensitive droplet-digital PCR technology and qRT-PCR were utilized to study the clinically derived and in vitro samples, respectively. H4R was challenged with the specific agonist HST-10 and inverse agonist ST-1007. HBD-2 was highly induced in OLP lesions. In contrast, hBD2 expression was attenuated in OTSCC tissues, while very low levels of hBD-2 messenger RNA (mRNA) were observed in OTSCC cells. Together with tumor necrosis factor-α (TNF-α), histamine upregulated hBD-2 mRNA expression in HOKs. Activation of H4R seems to modulate the expression of epithelial hBD-2. These findings suggest the involvement of hBD-2 in the pathogenesis of OLP and may, thus, be harnessed for therapeutic interventions in OLP.
The Emerging Role of Electrophiles as a Key Regulator for Endoplasmic Reticulum (ER) Stress
Authors: Takasugi Nobumasa , Hiraoka Hideki , Nakahara Kengo , Akiyama Shiori , Fujikawa Kana , Nomura Ryosuke , Furuichi Moeka , Uehara Takashi
Published: April 2019

The unfolded protein response (UPR) is activated by the accumulation of misfolded proteins in the endoplasmic reticulum (ER), which is called ER stress. ER stress sensors PERK, IRE1, and ATF6 play a central role in the initiation and regulation of the UPR; they inhibit novel protein synthesis and upregulate ER chaperones, such as protein disulfide isomerase, to remove unfolded proteins. However, when recovery from ER stress is difficult, the UPR pathway is activated to eliminate unhealthy cells. This signaling transition is the key event of many human diseases. However, the precise mechanisms are largely unknown. Intriguingly, reactive electrophilic species (RES), which exist in the environment or are produced through cellular metabolism, have been identified as a key player of this transition. In this review, we focused on the function of representative RES: nitric oxide (NO) as a gaseous RES, 4-hydroxynonenal (HNE) as a lipid RES, and methylmercury (MeHg) as an environmental organic compound RES, to outline the relationship between ER stress and RES. Modulation by RES might be a target for the development of next-generation therapy for ER stress-associated diseases.
Assessing Lisbon Trees’ Carbon Storage Quantity, Density, and Value Using Open Data and Allometric Equations
Authors: Neto Miguel de Castro , Sarmento Pedro
Published: April 2019

Urban population has grown exponentially in recent years, leading to an increase of CO2 emissions and consequently contributing on a large scale to climate change. Urban trees are fundamental to mitigating CO2 emissions as they incorporate carbon in their biomass. It becomes necessary to understand and measure urban tree carbon storage. In this paper is studied the potential of open data to measure the quantity, density, and value of carbon stored by the seven most represented urban trees in the city of Lisbon. To compute carbon storage, the seven most represented urban tree species were selected from an open database acquired from an open data portal of the city of Lisbon. Through allometric equations, it was possible to compute the trees’ biomass and calculate carbon storage quantity, density, and value. The results showed that the tree species Celtis australis is the species that contributes more to carbon storage. Central parishes of the city of Lisbon present higher-density values of carbon storage when compared with the border parishes despite the first ones presenting low-to-medium values of carbon storage quantity and value. Trees located in streets, present higher values of carbon storage, when compared with trees located in schools and green areas. Finally, the potential usage of this information to build a decision-support dashboard for planning green infrastructures was demonstrated.
Predict Electric Power Demand with Extended Goal Graph and Heterogeneous Mixture Modeling
Authors: Kushiro Noriyuki , Fukuda Ami , Kawatsu Masatada , Mega Toshihiro
Published: April 2019

In this study, methods for predicting energy demand on hourly consumption data are established for realizing an energy management system for buildings. The methods consist of an energy prediction algorithm that automatically separates the datasets to partitions (gate) and creates a linear regression model (local expert) for each partition on the heterogeneous mixture modeling, and an extended goal graph that extracts candidates of variables both for data partitioning and for linear regression for the energy prediction algorithm. These methods were implemented as tools and applied to create the energy prediction model on two years' hourly consumption data for a building. We validated the methods by comparing accuracies with those of different machine learning algorithms applied to the same datasets.
Stability Assessment of Earth Retaining Structures under Static and Seismic Conditions
Authors: Nimbalkar Sanjay , Pain Anindya , Ahmad Syed Mohd , Chen Qingsheng
Published: April 2019

An accurate estimation of static and seismic earth pressures is extremely important in geotechnical design. The conventional Coulomb’s approach and Mononobe-Okabe’s approach have been widely used in engineering practice. However, the latter approach provides the linear distribution of seismic earth pressure behind a retaining wall in an approximate way. Therefore, the pseudo-dynamic method can be used to compute the distribution of seismic active earth pressure in a more realistic manner. The effect of wall and soil inertia must be considered for the design of a retaining wall under seismic conditions. The method proposed considers the propagation of shear and primary waves through the backfill soil and the retaining wall due to seismic excitation. The crude estimate of finding the approximate seismic acceleration makes the pseudo-static approach often unreliable to adopt in the stability assessment of retaining walls. The predictions of the active earth pressure using Coulomb theory are not consistent with the laboratory results to the development of arching in the backfill soil. A new method is proposed to compute the active earth pressure acting on the backface of a rigid retaining wall undergoing horizontal translation. The predictions of the proposed method are verified against results of laboratory tests as well as the results from other methods proposed in the past.
Development of an Oil Free Water-Based Lubricant for Gear Applications
Authors: Sagraloff Nadine , Dobler Andreas , Tobie Thomas , Stahl Karsten , Ostrowski Jens
Published: April 2019

The aim of the current research is to develop a gear transmission fluid based on water and plant extract. Up to now, mineral or synthetic oils are used as lubricants in most gear drive applications. These oils are made of fossil raw materials and are non-biodegradable. Lately, there have been some efforts made to develop lubricants as an alternative to conventional lubrication systems such as triglycerides from native oils or synthetic esters. These lubricants are more biodegradable than mineral oils but also show some difficulties during performance like saponification. Within a former research project, the company Carl Bechem GmbH and the Fraunhofer Institute for Process Engineering and Packaging IVV developed a cutting fluid based on water and plant extract. With a model sample of this fluid, which also contained gear typical additives, preliminary experimental investigations for a current research study were conducted. The results confirmed the general suitability of this water-based lubricant for gear drives under certain operating conditions. Using water as lubricants can lead to some improved characteristics due to the very high thermal conductivity and the reduced friction. This paper aims to point out the benefits of using water-based lubricants, share the results of some preliminary experimental investigations on a fluid sample based on water and plant extract, and discuss the challenges, which one has to overcome during the development of such new lubricants.
Influence of New Technologies on Post-Stroke Rehabilitation: A Comparison of Armeo Spring to the Kinect System
Authors: Adomavičienė Aušra , Daunoravičienė Kristina , Kubilius Raimondas , Varžaitytė Lina , Raistenskis Juozas
Published: April 2019

Background: New technologies to improve post-stroke rehabilitation outcomes are of great interest and have a positive impact on functional, motor, and cognitive recovery. Identifying the most effective rehabilitation intervention is a recognized priority for stroke research and provides an opportunity to achieve a more desirable effect. Objective: The objective is to verify the effect of new technologies on motor outcomes of the upper limbs, functional state, and cognitive functions in post-stroke rehabilitation. Methods: Forty two post-stroke patients (8.69 ± 4.27 weeks after stroke onset) were involved in the experimental study during inpatient rehabilitation. Patients were randomly divided into two groups: conventional programs were combined with the Armeo Spring robot-assisted trainer (Armeo group; n = 17) and the Kinect-based system (Kinect group; n = 25). The duration of sessions with the new technological devices was 45 min/day (10 sessions in total). Functional recovery was compared among groups using the Functional Independence Measure (FIM), and upper limbs’ motor function recovery was compared using the Fugl–Meyer Assessment Upper Extremity (FMA-UE), Modified Ashworth Scale (MAS), Hand grip strength (dynamometry), Hand Tapping test (HTT), Box and Block Test (BBT), and kinematic measures (active Range Of Motion (ROM)), while cognitive functions were assessed by the MMSE (Mini-Mental State Examination), ACE-R (Addenbrooke’s Cognitive Examination-Revised), and HAD (Hospital Anxiety and Depression Scale) scores. Results: Functional independence did not show meaningful differences in scores between technologies (p > 0.05), though abilities of self-care were significantly higher after Kinect-based training (p < 0.05). The upper limbs’ kinematics demonstrated higher functional recovery after robot training: decreased muscle tone, improved shoulder and elbow ROMs, hand dexterity, and grip strength (p < 0.05). Besides, virtual reality games involve more arm rotation and performing wider movements. Both new technologies caused an increase in overall global cognitive changes, but visual constructive abilities (attention, memory, visuospatial abilities, and complex commands) were statistically higher after robotic therapy. Furthermore, decreased anxiety level was observed after virtual reality therapy (p < 0.05). Conclusions: Our study displays that even a short-term, two-week training program with new technologies had a positive effect and significantly recovered post-strokes functional level in self-care, upper limb motor ability (dexterity and movements, grip strength, kinematic data), visual constructive abilities (attention, memory, visuospatial abilities, and complex commands) and decreased anxiety level.
Protonic Conduction of Partially-Substituted CsH2PO4 and the Applicability in Electrochemical Devices
Authors: Navarrete Laura , Andrio Andreu , Escolástico Sonia , Moya Sergio , Compañ Vicente , Serra José M.
Published: April 2019

CsH2PO4 is a proton conductor pertaining to the acid salts group and shows a phase transition from monoclinic to cubic phase at 232 ± 2 °C under high-steam atmospheres (>30%). This cubic phase gives rise to the so-called superprotonic conductivity. In this work, the influence of the partial substitution of Cs by Ba and Rb, as well as the partial substitution of P by W, Mo, and S in CsH2PO4 on the phase transition temperature and electrochemical properties is studied. Among the tested materials, the partial substitution by Rb led to the highest conductivity at high temperature. Furthermore, Ba and S-substituted salts exhibited the highest conductivity at low temperatures. CsH2PO4 was used as electrolyte in a fully-assembled fuel cell demonstrating the applicability of the material at high pressures and the possibility to use other materials (Cu and ZnO) instead of Pt as electrode electrocatalyst. Finally, an electrolyzer cell composed of CsH2PO4 as electrolyte, Cu and ZnO as cathode and Pt and Ag as anode was evaluated, obtaining a stable production of H2 at 250 °C.
Dynamic Uniform Deformation for Electromagnetic Uniaxial Tension
Authors: Cui Xiaohui , Zhang Zhiwu , Yu Hailiang , Cheng Yongqi , Xiao Xiaoting
Published: April 2019

To compare with quasi-static uniaxial tensioning, researchers designed an electromagnetic uniaxial tension method using a runway coil. However, the requirements to obtain a uniformly deformed sample and the ways the stress changes on the sample using a runway coil have not been studied in the past. In this study, a three-dimensional (3D) sequential coupling method was developed to analyze the factors affecting on-sheet deformation inhomogeneity under electromagnetic uniaxial tension. Two main process parameters, comprising the die type and the relative position of the coil and sheet, were evaluated. Under the optimal parameters, the experiment and simulation both obtained uniformly deformed samples with different discharge conditions, and the simulation method had a high accuracy in modeling the deformation process. The stress state of the sample is approximately unidirectional tensile stress before 240 μs. After 240 μs, the three main stresses showed significant oscillations.
Swell–Shrink Behavior of Rubberized Expansive Clays during Alternate Wetting and Drying
Authors: Soltani Amin , Deng An , Taheri Abbas , Mirzababaei Mehdi , Vanapalli Sai K.
Published: April 2019

The present study examines rubber’s capacity of improving the swell–shrink potential of expansive clays. Two rubber types of fine and coarse categories with different geometrical features were considered. The test program consisted of standard Proctor compaction and cyclic wetting–drying tests. Scanning electron microscopy (SEM) analysis was also performed to identify the soil–rubber amending mechanisms, and to observe the evolution of fabric in response to alternate wetting and drying. Cyclic wetting–drying led to the reconstruction of the soil/soil–rubber microstructure by way of inducing aggregation and cementation of the soil grains. The greater the number of applied cycles, the lower the swell–shrink features, following a monotonically decreasing trend, with the rubberized blends holding a notable advantage over the virgin soil. The tendency for reduction, however, was in favor of a larger rubber size, and more importantly the rubber’s elongated form factor; thus, predicating a rubber size/shape-dependent amending mechanism. The soil–rubber amending mechanisms were discussed in three aspects—increase in non-expansive content, frictional resistance generated as a result of soil–rubber contact, and mechanical interlocking of rubber particles and soil grains. The swell–shrink patterns/paths indicated an expansive accumulated deformation for the virgin soil, whereas the rubberized blends manifested a relatively neutral deformational state, thereby corroborating the rubber’s capacity to counteract the heave and/or settlement incurred by alternate wetting and drying.
Coprecipitation of Co2+, Ni2+ and Zn2+ with Mn(III/IV) Oxides Formed in Metal-Rich Mine Waters
Authors: Sánchez-España Javier , Yusta Iñaki
Published: April 2019

Manganese oxides are widespread in soils and natural waters, and their capacity to adsorb different trace metals such as Co, Ni, or Zn is well known. In this study, we aimed to compare the extent of trace metal coprecipitation in different Mn oxides formed during Mn(II) oxidation in highly concentrated, metal-rich mine waters. For this purpose, mine water samples collected from the deepest part of several acidic pit lakes in Spain (pH 2.7–4.2), with very high concentration of manganese (358–892 mg/L Mn) and trace metals (e.g., 795–10,394 µg/L Ni, 678–11,081 µg/L Co, 259–624 mg/L Zn), were neutralized to pH 8.0 in the laboratory and later used for Mn(II) oxidation experiments. These waters were subsequently allowed to oxidize at room temperature and pH = 8.5–9.0 over several weeks until Mn(II) was totally oxidized and a dense layer of manganese precipitates had been formed. These solids were characterized by different techniques for investigating the mineral phases formed and the amount of coprecipitated trace metals. All Mn oxides were fine-grained and poorly crystalline. Evidence from X-Ray Diffraction (XRD) and Scanning Electron Microscopy coupled to Energy Dispersive X-Ray Spectroscopy (SEM–EDX) suggests the formation of different manganese oxides with varying oxidation state ranging from Mn(III) (e.g., manganite) and Mn(III/IV) (e.g., birnessite, todorokite) to Mn(IV) (e.g., asbolane). Whole-precipitate analyses by Inductively Coupled Plasma-Mass Spectrometry (ICP-MS), Inductively Coupled Plasma-Atomic Emission Spectrometry (ICP-AES), and/or Atomic Absorption Spectrometry (AAS), provided important concentrations of trace metals in birnessite (e.g., up to 1424 ppm Co, 814 ppm Ni, and 2713 ppm Zn), while Co and Ni concentrations at weight percent units were detected in asbolane by SEM-EDX. This trace metal retention capacity is lower than that observed in natural Mn oxides (e.g., birnessite) formed in the water column in a circum-neutral pit lake (pH 7.0–8.0), or in desautelsite obtained in previous neutralization experiments (pH 9.0–10.0). However, given the very high amount of Mn sorbent material formed in the solutions (2.8–4.6 g/L Mn oxide), the formation of these Mn(III/IV) oxides invariably led to the virtually total removal of Co, Ni, and Zn from the aqueous phase. We evaluate these data in the context of mine water pollution treatment and recovery of critical metals.
Curcumin: Total-Scale Analysis of the Scientific Literature
Authors: Yeung Andy Wai Kan , Horbańczuk Michal , Tzvetkov Nikolay T. , Mocan Andrei , Carradori Simone , Maggi Filippo , Marchewka Joanna , Sut Stefania , Dall’Acqua Stefano , Gan Ren-You , Tancheva Lyubka P. , Polgar Timea , Berindan-Neagoe Ioana , Pirgozliev Vasil , Šmejkal Karel , Atanasov Atanas G.
Published: April 2019

The current study aimed to provide a comprehensive bibliometric overview of the literature on curcumin, complementing the previous reviews and meta-analyses on its potential health benefits. Bibliometric data for the current analysis were extracted from the Web of Science Core Collection database, using the search string TOPIC=(“curcumin*”), and analyzed by the VOSviewer software. The search yielded 18,036 manuscripts. The ratio of original articles to reviews was 10.4:1. More than half of the papers have been published since 2014. The major contributing countries were the United States, China, India, Japan, and South Korea. These publications were mainly published in journals representing the following scientific disciplines: biochemistry, chemistry, oncology, and pharmacology. There was a significant positive correlation between the total publication count and averaged citations per manuscript for affiliations, but not for countries/regions and journals. Chemicals that were frequently mentioned in the keywords of evaluated curcumin publications included curcuminoids, resveratrol, chitosan, flavonoids, quercetin, and polyphenols. The literature mainly focused on curcumin’s effects against cancer, inflammation, and oxidative stress. Cancer types most frequently investigated were breast, colon, colorectal, pancreatic, and prostate cancers.
Correlation between Chemical Composition and Antifungal Activity of Clausena lansium Essential Oil against Candida spp.
Authors: He Xiaowen , Zhang Lantong , Chen Jinping , Sui Jinlei , Yi Guohui , Wu Jinyan , Ma Yinzheng
Published: April 2019

Essential oils (EOs) have been shown to have a diversity of beneficial human health effects. Clausena is a large and highly diverse genus of plants with medicinal and cosmetic significance. The aim of this study was to analyze the composition of Clausena lansium EOs and to investigate their potential antifungal effects. The chemical compositions of Clausena lansium EOs obtained by hydrodistillation were analyzed by gas chromatography-mass spectrometry (GC-MS). A total of 101 compounds were identified among the diverse extracts of C. lansium. EOs of leaves and pericarps from different cultivars (Hainan local wampee and chicken heart wampee) collected in Hainan (China) were classified into four clusters based on their compositions. These clusters showed different antifungal activities against five Candida species (C. albicans, C. tropicalis, C. glabrata, C. krusei and C. parapsilosis) using the disc diffusion method. Clausena lansium EOs of pericarps displayed noteworthy antifungal activitives against all the tested Candida strains with inhibition zone diameters in the range of 11.1–23.1 mm. EOs of leaves showed relatively low antifungal activities with inhibition zone diameters in the range of 6.5–22.2 mm. The rank order of antifungal activities among the four EO clusters was as follows: Cluster IV> Cluster III > Cluster I ≥ Cluster II. These results represent the first report about the correlation between chemical composition of C. lansium EOs and antifungal activity. Higher contents of β-phellandrene, β-sesquiphellandrene and β-bisabolene in EOs of pericarps were likely responsible for the high antifungal activity of Cluster IV EOs. Taken together, our results demonstrate the chemical diversity of Clausena lansium EOs and their potential as novel antifungal agents for candidiasis caused by Candida spp. Furthermore, the obtained results showing a wide spectrum of antifungal activities provide scientific evidence for the traditional use of these plants.
Spermidine Enhances Heat Tolerance of Rice Seeds by Modulating Endogenous Starch and Polyamine Metabolism
Authors: Fu Yuying , Gu Qingqing , Dong Qian , Zhang Zhihao , Lin Cheng , Hu Weimin , Pan Ronghui , Guan Yajing , Hu Jin
Published: April 2019

Polyamines have been reported to be involved in grain filling and they might contribute to the construction of heat resistance of some cereals. In this study, the hybrid rice ‘YLY 689’ was used to explore the possible effects of exogenous spermidine (Spd) on seed quality under high temperature during the filling stage. Rice spikes were treated with Spd or its synthesis inhibitor cyclohexylamine (CHA) after pollination, and then the rice plants were transferred to 40 °C for 5-day heat treatment. The results showed that, compared with the control under high temperature, Spd pretreatment significantly improved the germination percentage, germination index, vigor index, seedling shoot height, and dry weight of seeds harvested at 35 days after pollination, while the CHA significantly decreased the seed germination and seedling growth. Meanwhile, Spd significantly increased the peroxidase (POD) activity and decreased the malondialdehyde (MDA) content in seeds. In addition, after spraying with Spd, the endogenous content of spermidine and spermine and the expression of their synthetic genes, spermidine synthase (SPDSYN) and spermine synthase (SPMS1 and SPMS2), significantly increased, whereas the accumulation of amylose and total starch and the expression of their related synthase genes, soluble starch synthase II-3 (SS II-3) and granules bound starch synthase I (GBSSI), also increased to some extent. The data suggests that exogenous Spd pretreatment could alleviate the negative impacts of high temperature stress on rice seed grain filling and improve the rice seed quality to some extent, which might be partly caused by up-regulating endogenous polyamines and starch metabolism.
Introduction to the Molecules Special Edition Entitled ‘Heparan Sulfate and Heparin: Challenges and Controversies’: Some Outstanding Questions in Heparan Sulfate and Heparin Research
Authors: Yates Edwin A , Gallagher John T , Guerrini Marco
Published: April 2019

The scope of this article is to provide a brief general introduction to heparan sulfate (HS) and heparin, and attempt to identify some of the central challenges regarding research into the chemistry and biology of glycosaminoglycans (GAGs), some of which are the subject of contributions to the special issue of Molecules (published in volume 23, 2018) entitled ‘Heparan Sulfate and Heparin: Challenges and Controversies’ [...]
Compositional Consequences of Partial Dealcoholization of Red Wine by Reverse Osmosis-Evaporative Perstraction
Authors: Pham Duc-Truc , Stockdale Vanessa J. , Wollan David , Jeffery David W. , Wilkinson Kerry L.
Published: April 2019

This study investigated compositional changes in red wines resulting from wine alcohol removal by reverse osmosis-vaporative perstraction (RO-EP) and provides insight into the physical and chemical changes in reduced alcohol wine (RAW). Trial 1 involved RO-EP treatment of three wines that were analyzed pre-treatment, post-treatment, and post-treatment with alcohol adjustment (i.e., addition of ethanol to achieve the original alcohol content). Trial 2 involved partial dealcoholization of two wines and analysis of samples collected during RO-EP treatment, i.e., wine in, wine out, retentate, permeate (pre- and post-EP treatment) and strip water. Wine color was analyzed by spectrophotometric methods, while other compositional changes were determined by WineScan, high performance liquid chromatography (HPLC) and gas chromatography–mass spectrometry (GC–MS) analyses. In general, RAWs were slightly more concentrated than pre-treatment wines, which resulted in greater color intensity and increased phenolics and organic acids. However, partial dealcoholization resulted in lower concentrations of some fermentation volatiles, particularly ethyl esters, which may reflect ester hydrolysis following ethanol removal.
Effect of Sonication Duration in the Performance of Polyvinyl Alcohol/Chitosan Bilayer Films and Their Effect on Strawberry Preservation
Authors: Ding Jie , Zhang Rong , Ahmed Saeed , Liu Yaowen , Qin Wen
Published: April 2019

In this study, we fabricated polyvinyl alcohol (PVA)/chitosan (CS) bilayer films by casting and investigated the effects of preparation conditions and CS content (2, 2.5, or 3 wt.%) on the ability of these films to preserve packaged strawberries. The best performance was achieved at a CS loading of 2.5 wt.% (ultrasound time, 25 min); the strain and stress values were 143.15 ± 6.43% and 70.67 ± 0.85 MPa, respectively, oxygen permeability was 0.16 ± 0.08 cm2·m2·day−1·MPa−1, water vapor permeability was 14.93 ± 4.09 g·cm−1·s−1·Pa−1, and the shelf life of fresh strawberries packaged in the PVA/CS 2.5 wt.% bilayer film was determined to be 21 days at 5 ± 2 °C and a relative humidity of 60 ± 5%. Treatment with PVA/CS bilayer films prevented the decrease in the firmness of strawberries during storage (21 days). The evaluated physicochemical parameters (weight loss, decay, firmness, titratable acidity, soluble solid content, ascorbic acid content, and color) indicated that treatment with PVA/CS bilayer films led to better maintenance of the fruit quality. We believe that our study makes a significant contribution to literature because it paves the way to the fabrication of smart packaging materials and facilitates the commercialization of fresh strawberries as an important health food.
Enzymatic Weight Update Algorithm for DNA-Based Molecular Learning
Authors: Baek Christina , Lee Sang-Woo , Lee Beom-Jin , Kwak Dong-Hyun , Zhang Byoung-Tak
Published: April 2019

Recent research in DNA nanotechnology has demonstrated that biological substrates can be used for computing at a molecular level. However, in vitro demonstrations of DNA computations use preprogrammed, rule-based methods which lack the adaptability that may be essential in developing molecular systems that function in dynamic environments. Here, we introduce an in vitro molecular algorithm that ‘learns’ molecular models from training data, opening the possibility of ‘machine learning’ in wet molecular systems. Our algorithm enables enzymatic weight update by targeting internal loop structures in DNA and ensemble learning, based on the hypernetwork model. This novel approach allows massively parallel processing of DNA with enzymes for specific structural selection for learning in an iterative manner. We also introduce an intuitive method of DNA data construction to dramatically reduce the number of unique DNA sequences needed to cover the large search space of feature sets. By combining molecular computing and machine learning the proposed algorithm makes a step closer to developing molecular computing technologies for future access to more intelligent molecular systems.
MicroRNA-181c Inhibits Interleukin-6-mediated Beta Cell Apoptosis by Targeting TNF-α Expression
Authors: Oh Yoon Sin , Bae Gong Deuk , Park Eun-Young , Jun Hee-Sook
Published: April 2019

We have previously reported that long-term treatment of beta cells with interleukin-6 (IL-6) is pro-apoptotic. However, little is known about the regulatory mechanisms that are involved. Therefore, we investigated pro-apoptotic changes in mRNA expression in beta cells in response to IL-6 treatment. We analyzed a microarray with RNA from INS-1 beta cells treated with IL-6, and found that TNF-α mRNA was significantly upregulated. Inhibition of TNF-α expression by neutralizing antibodies significantly decreased annexin V staining in cells compared with those treated with a control antibody. We identified three microRNAs that were differentially expressed in INS-1 cells incubated with IL-6. In particular, miR-181c was significantly downregulated in IL-6-treated cells compared with control cells and the decrease of miR-181c was attenuated by STAT-3 signaling inhibition. TNF-α mRNA was a direct target of miR-181c and upregulation of miR-181c by mimics, inhibited IL-6-induced increase in TNF-α mRNA expression. Consequently, reduction of TNF-α mRNA caused by miR-181c mimics enhanced cell viability in IL-6 treated INS-1 cells. These results demonstrated that miR-181c regulation of TNF-α expression plays a role in IL-6-induced beta cell apoptosis.