bluuebunny/crossref_metadata_2025 huggingface dataset:
Development and Validation of a Masking System for Mitigation of Low-Frequency Audible Noise from Electrical Substations
Authors: Regazzi Rogerio , Cunha Brunno , de Miranda Hugo Villela , Acosta Juan José Gómez , Barbosa Carlos Roberto Hall , Frota Mauricio Nogueira , Souza João Vitor , Gomes Carlos Augusto Machado
Published: August 2021

Low-frequency audible noise generated by the magnetostriction effect inherent to the operation of power transformers has become a major drawback, especially in cases where the electrical substation is located in urban areas subject to strict environmental regulations that impose noise limits, differing for day and night periods. Such regulations apply a +5 dB penalty if a tonal component of noise is present, which is clearly the case of magnetostriction noise, typically concentrated at twice the industrial frequency (50 Hz or 60 Hz, depending on the country). The strategy used to eliminate the tonal characteristics, therefore contributing to establish compliance with the applicable regulation and to alleviate the discomfort it causes to the human ear, consisted in superimposing to the substation noise a masking sound synthesized from “sounds of nature” with suitable intensities, to flatten the noise spectrum while enhancing the soundscape. The masking system (heavy-duty speakers powered by a microprocessor platform) was validated at an already judicialized urban scenario. Measurement results confirmed that the masking solution was capable of flattening the tonal frequencies, whose beneficial effect yielded the cancellation of the public civil action filed by the neighbors. The proposed solution is ready to be replicated in other scenarios.
An Insightful Overview of the Wiener Filter for System Identification
Authors: Dogariu Laura-Maria , Benesty Jacob , Paleologu Constantin , Ciochină Silviu
Published: August 2021

Efficiently solving a system identification problem represents an important step in numerous important applications. In this framework, some of the most popular solutions rely on the Wiener filter, which is widely used in practice. Moreover, it also represents a benchmark for other related optimization problems. In this paper, new insights into the regularization of the Wiener filter are provided, which is a must in real-world scenarios. A proper regularization technique is of great importance, especially in challenging conditions, e.g., when operating in noisy environments and/or when only a low quantity of data is available for the estimation of the statistics. Different regularization methods are investigated in this paper, including several new solutions that fit very well for the identification of sparse and low-rank systems. Experimental results support the theoretical developments and indicate the efficiency of the proposed techniques.
Enhancement of Desulfurization Capacity with Cu-Based Macro-Porous Sorbents for Hydrogen Production by Gasification of Petroleum Cokes
Authors: Kim Dongjoon , Bae Dasol , Kim Yu Jin , Lee Seung Jong , Lee Jin Wook , Yun Yongseung , Park No-Kuk , Kim Minkyu
Published: August 2021

Macro-porous alumina was used as a support for a pellet-type Cu-based desulfurization sorbent in the gas purification process for producing blue hydrogen by the gasification of petroleum coke. The effects of the macro-porous alumina on the pellet-type sorbents in reducing the gas diffusion resistance into the pores were investigated. The results showed that the macro-porous alumina enhances the diffusion resistance, resulting in an improved sulfur capacity of CuO absorbents. Such effects were more significant on the pellet type CuO absorbents than the powder type. In addition, CO production was observed experimentally during the desulfurization reaction of carbonyl sulfide (COS) at low temperatures (~473 K). Density functional theory calculations were also performed to understand the kinetics of desulfurization and CO production. The simulation results predicted that the kinetics of desulfurization is strongly affected by the local surface environment. The CO generated from C–O bond breaking from COS had a lower adsorption energy than the CO2 formation. These results suggest that the Cu-based desulfurization sorbent has potential catalytic activity for producing CO from COS dissociation.
Effect of Plant Biostimulants on Nutritional and Chemical Profiles of Almond and Hazelnut
Authors: Pascoalino Liege A. , Reis Filipa S. , Barros Lillian , Rodrigues Manuel Ângelo , Correia Carlos M. , Vieira Admilson L. , Ferreira Isabel C. F. R. , Barreira João C. M.
Published: August 2021

The increasing interest in natural foods with functional effects demands progressively higher production levels. Nonetheless, there is an orientation towards practicing more sustainable agriculture, free from environmentally harmful pesticides and fertilizers. Plant biostimulants, a class of bio-based agriculture products designed to improve crop development, represent a feasible alternative to chemical fertilizers, or, at least, an effective way of reducing the employed quantities. Herein, different types of plant biostimulants compatible with organic farming (Phytoalgae, Foliar B, Amino Acids, Soil B, Fitoalgas Green® and Sprint Plus®) were tested in two of the most important nut products worldwide: almonds and hazelnuts, which were tested for nutritional parameters, fatty acids profiles and tocopherols contents. Overall, the most notorious effects in almond samples were obtained with phytoalgae (seaweed Ascophyllum nodosum extracts), particularly reflected in the upraising around 10% of γ-tocopherol and β-tocopherol contents. Likewise, hazelnuts treated with NPK + phytoalgae were also characterized by an increase of almost 18% in tocopherols levels, while treatment with NPK alone induced 15.1% higher percentage of linoleic acid.
Power Losses Minimization for Optimal Operating Maps in Power-Split HEVs: A Case Study on the Chevrolet Volt
Authors: Castellano Antonella , Cammalleri Marco
Published: August 2021

The power-split architecture is the most promising hybrid electric powertrain. However, a real advantage in energy saving while maintaining high performance can be achieved only by the implementation of a proper energy management strategy. This requires an optimized functional design before and a comprehensive analysis of the powertrain losses after, which could be rather challenging owing to the constructive complexity of the power-split transmission, especially for multi-mode architecture with multiple planetary gearing. This difficulty was overcome by a dimensionless model, already available in the literature, that enables the analysis of any power-split transmission, even in full electric operation. This paper relies on this approach to find the operating points of the internal combustion engine and both electric machines which minimize the total power losses. This optimization is carried out for given vehicle speed and demanded torque, by supposing different scenarios in respect of the battery capability of providing or gathering power. The efficiency of the thermal engine and the electric machines is considered, as well as the transmission mechanical power losses. The aim is to provide a global efficiency map that can be exploited to extract data for the implementation of the most suitable real-time control strategy. As a case study, the procedure is applied to the multi-mode power-split system of the Chevrolet Volt.
Development and Application of MAGIC-f Gel in Cancer Research and Medical Imaging
Authors: Dhakal Rabin , Yosofvand Mohammad , Moussa Hanna
Published: August 2021

Much of the complex medical physics work requires radiation dose delivery, which requires dosimeters to accurately measure complex three-dimensional dose distribution with good spatial resolution. MAGIC-f polymer gel is one of the emerging new dosimeters widely used in medical physics research. The purpose of this study was to present an overview of polymer gel dosimetry, using MAGIC-f gel, including its composition, manufacture, imaging, calibration, and application to medical physics research. In this review, the history of polymer gel development is presented, along with the applications so far. Moreover, the most important experiments/applications of MAGIC-f polymer gel are discussed to illustrate the behavior of gel on different conditions of irradiation, imaging, and manufacturing techniques. Finally, various future works are suggested based on the past and present works on MAGIC-f gel and polymer gel in general, with the hope that these bits of knowledge can provide important clues for future research on MAGIC-f gel as a dosimeter.
Optimization of Multi-Blade Centrifugal Fan Blade Design for Ventilation and Air-Conditioning System Based on Disturbance CST Function
Authors: Zhou Shuiqing , Yang Ke , Zhang Weitao , Zhang Kai , Wang Chihu , Jin Weiya
Published: August 2021

The multi-blade centrifugal fan is commonly used in modern building ventilation and air-conditioning system. However, it does not readily satisfy the increasing demand for energy saving, high efficiency or noise reduction. Its performance is inherently limited by the geometrical structure of single circular arc blades. Q35-type multi-blade centrifugal fan studied as an example by combining the disturbance CST function to parameterize the blades. The optimization parameter change range is confirmed, and test samples are extracted before establishing an RBF proxy model. The NSGA-II algorithm is incorporated, and multi-objective optimization is performed with flow rate and total pressure efficiency as optimization goals. The results show that the fan performance is effectively improved. At the design working point, the air volume of the multi-blade centrifugal fan increases by 1.4 m3/min; at the same time, the total pressure efficiency increases by 3.1%, and the noise is reduced by 1.12 dB, applying the proposed design. The obtained higher fan efficiency can effectively improve performance of the whole ventilation and air-conditioning system. This novel optimization method also has relatively few parameters, which makes it potentially valuable for designing multi-wing centrifugal and other types of fans, providing a new idea for energy saving and emission reduction design of fan.
Design of an IMCPID Optimized Neural Network for Stepless Flow Control of Reciprocating Mechinery
Authors: Hong Huaibin , Jiang Zhinong , Ma Wensheng , Xiong Wei , Zhang Jinjie , Liu Wenhua , Wang Yao
Published: August 2021

It is usually difficult to design a controller for a nonlinear multiple-input and multiple-output (MIMO) system. The methodological approach taken in this study is a mixed methodology based on a PID-type internal model control (IMC) method and neural network (NN) optimization algorithm. The NN controller is designed for adjusting the sole parameter in IMCPID and compensating the characteristic changes and non-linearity in stepless flow control. In this study, a simulation of a nonlinear MIMO system with strong coupling is carried out. The simulation results indicate that the proposed control method has a better performance in settle time, overshoot, robustness and set-point tracking accuracy compared with other considered methods.
Three-Dimensional Imaging of Metallic Grain by Stacking the Microscopic Images
Authors: Lee Jinyi , Berkache Azouaou , Wang Dabin , Hwang Young-Ha
Published: August 2021

Three-dimensional observation of metal grains (MG) has a wide potential application serving the interdisciplinary community. It can be used for industrial applications and basic research to overcome the limitations of non-destructive testing methods, such as ultrasonic testing, magnetic particle testing, and eddy current testing. This study proposes a method and its implementation algorithm to observe (MG) metal grains in three dimensions in a general laboratory environment equipped with a polishing machine and a metal microscope. An image was taken by a metal microscope while polishing the mounted object to be measured. Then, the metal grains (MGs) were reconstructed into three dimensions through local positioning, binarization, boundary extraction, (MG) selection, and stacking. The goal is to reconstruct the 3D MG in a virtual form that reflects the real shape of the MG. The usefulness of the proposed method was verified using the carbon steel (SA106) specimen.
Experimental Study with Cement Mortars Made with Recycled Concrete Aggregate and Reinforced with Aramid Fibers
Authors: Morón Alberto , Ferrández Daniel , Saiz Pablo , Morón Carlos
Published: August 2021

The reuse of construction and demolition waste is a necessary way to achieve greater sustainability in building, introducing the criteria of the so-called circular economy in the design of the production process of new construction materials. This research focuses on analyzing the properties of mortars made with recycled aggregates from concrete waste and reinforced with aramid fibers. For this purpose, an experimental campaign was carried out, including chemical, physical, mechanical and durability tests, performing a statistical analysis to discuss the different properties analyzed. The results show how the incorporation of aramid fibers in the matrix of cement mortars made with recycled concrete aggregate improves their technical performance and mechanical resistance, thus increasing their application possibilities and achieving similar results in some properties to those obtained with traditional mortars made with natural aggregate.
Leakage Position Estimation of Cooling Water Using a Stereo Camera for Fukushima Daiichi Nuclear Power Plant
Authors: Xu Hao , Komatsu Ren , Woo Hanwool , Yamashita Atsushi , Asama Hajime
Published: August 2021

In this study, a new method of estimating the leakage positions of cooling water using a stereo camera for the Fukushima Daiichi Nuclear Power Plant (FDNPP) is proposed. A stereo camera mounted on an inspection system with a rotating base was inserted into the pedestal below the reactor pressure vessel (RPV), and the waterdrops from the leakage position were captured using a stereo camera. We estimated the leakage positions by triangulating the waterdrop trajectory lines in the stereo image. The main contribution of this study is the extraction and matching of the waterdrop trajectories in a stereo image in the FDNPP. The radiation noise is intense inside the pedestal because of the presence of fuel debris. Therefore, we propose a method that is robust against radiation noise. We assume that the waterdrops drip vertically in indoor environments without wind, such as in the FDNPP. Hence, the orientation of the stereo camera can be adjusted by the rotating base such that the vertical lines in the three-dimensional space are also projected as vertical lines in the image planes. Thereafter, the columns of pixels in the images are treated as image features and used to extract and match the waterdrop trajectories. We demonstrated the effectiveness of our leakage position estimation method in a simulated environment of the FDNPP with gamma-ray image noise.
Failure Mechanism of Rock Specimens with a Notched Hole under Compression—A Numerical Study
Authors: Manouchehrian Amin , Kulatilake Pinnaduwa H. S. W. , Wu Rui
Published: August 2021

Discontinuities are natural structures that exist in rocks and can affect the stability of rock structures. In this article, the influence of notch presence on the strength and failure evolution around a hole in compressed rock specimens is investigated numerically. Firstly, the uniaxial compressive test on a rock specimen with a circular hole is modeled, and the failure evolution in the specimen is simulated. In a separate model, notches are created at the surface of the hole. Results show that, when the notches are created in the model, a failure zone around the hole is transferred to a distance away from the surface of the hole. In addition, a parametric study is carried out to investigate the influence of the notch length and the confining pressure on the fracturing behavior of the specimen. Numerical results presented in this article indicate that the presence of notches at the surface of the hole and their dimensions can affect the fracturing mechanism of the specimen. In some cases, the failure at the boundary of the hole is prevented when the notches of certain dimensions are added to the hole. The insights gained from this numerical study may be helpful to control the failure around underground excavations.
A Comparative Test on the Sensitivity of Freshwater and Marine Microalgae to Benzo-Sulfonamides, -Thiazoles and -Triazoles
Authors: Canova Luca , Sturini Michela , Maraschi Federica , Sangiorgi Stefano , Ferri Elida Nora
Published: August 2021

The evaluation of the ecotoxicological effects of water pollutants is performed by using different aquatic organisms. The effects of seven compounds belonging to a class of widespread contaminants, the benzo-fused nitrogen heterocycles, on a group of simple organisms employed in reference ISO tests on water quality (unicellular algae and luminescent bacteria) have been assessed to ascertain their suitability in revealing different contamination levels in the water, wastewater, and sediments samples. Representative compounds of benzotriazoles, benzothiazoles, and benzenesulfonamides, were tested at a concentration ranging from 0.01 to 100 mg L−1. In particular, our work was focused on the long-term effects, for which little information is up to now available. Species-specific sensitivity for any whole family of pollutants was not observed. On average, the strongest growth rate inhibition values were expressed by the freshwater Raphidocelis subcapitata and the marine Phaeodactylum tricornutum algae. R. subcapitata was the only organism for which growth was affected by most of the compounds at the lowest concentrations. The tests on the bioluminescent bacterium Vibrio fisheri gave completely different results, further underlining the need for an appropriate selection of the best biosensors to be employed in biotoxicological studies.
Phosphate Coatings: EIS and SEM Applied to Evaluate the Corrosion Behavior of Steel in Fire Extinguishing Solution
Authors: Burduhos-Nergis Diana-Petronela , Vasilescu Gabriel Dragos , Burduhos-Nergis Dumitru-Doru , Cimpoesu Ramona , Bejinariu Costica
Published: August 2021

Phosphate coatings are one of the most important methods in preventing metal corrosion, especially iron alloys. The high interest in this method for industrial applications is mainly due to the low costs associated with the phosphating solution component and technological process. Considering this aspect and the advantages offered to the materials (corrosion resistance, good adhesion, wear resistance), this study evaluates the potential of using the phosphate coating method in health and safety applications. Therefore, the deposition of a layer of zinc phosphate on the steel surface used in the manufacture of components for personal protective equipment was approached. Firefighters, during rescue/evacuation operations, use protective equipment for fall arrest, whose accessories (hooks, carabiners) are made of steel. Due to the low corrosion resistance property of carbon steel, these accessories must be replaced frequently. This paper aims to analyze the possibility of improving the corrosion resistance of carbon steel in a fire extinguishing solution. Accordingly, the electrochemical behavior of two different types of coatings was studied by electrochemical impedance spectroscopy (EIS) and scanning electron microscope (SEM). In the literature, different corrosion evaluating methods or systems have been considered, and up until now, there has been no previous study to have approached the corrosive behavior of C45, phosphate C45, and painted phosphate C45 immersed in fire extinguishing solution.
On Self-Aggregations of Min-Subgroups
Authors: Bejines Carlos , Ardanza-Trevijano Sergio , Elorza Jorge
Published: August 2021

Preservation of structures under aggregation functions is an active area of research with applications in many fields. Among such structures, min-subgroups play an important role, for instance, in mathematical morphology, where they can be used to model translation invariance. Aggregation of min-subgroups has only been studied for binary aggregation functions. However, results concerning preservation of the min-subgroup structure under binary aggregations do not generalize to aggregation functions with arbitrary input size since they are not associative. In this article, we prove that arbitrary self-aggregation functions preserve the min-subgroup structure. Moreover, we show that whenever the aggregation function is strictly increasing on its diagonal, a min-subgroup and its self-aggregation have the same level sets.
Comparison of Behavior and Space Use of the European Bullhead Cottus gobio and the Round Goby Neogobius melanostomus in a Simulated Natural Habitat
Authors: Roje Sara , Drozd Bořek , Richter Luise , Kubec Jan , Polívka Zdeněk , Worischka Susanne , Buřič Miloš
Published: August 2021

The round goby is an invasive fish in Europe and North America that threatens native species by predation and competition. Its habitat preferences are similar to those of the European bullhead, which it displaces from shelters and out-competes for available resources. We assessed the microhabitat preferences, shelter use, and activity of the round goby and European bullhead in single-species experiments in habitat simulator systems to investigate their behavior in a novel environment. Fish were video-recorded for 28 h in the presence of shelter and feed with water velocity ranging from 0.00 to 0.96 m s−1. The two species showed similar behavior under given conditions. A primary difference was in stress-induced behavior in the initial phases of observation. The round goby spent more time in movement when outside the shelter and a longer time in the escape zone in the exploration period during light. Our results confirmed a significant preference of round goby for low velocity areas and a preference for higher velocities in the European bullhead. Both species were able to cope with velocities > 0.7 m s−1. Therefore, the reported invasion success of round goby is probably not driven by space use or activity patterns, but rather by higher adaptability.
The Evolution of Trait Disparity during the Radiation of the Plant Genus Macrocarpaea (Gentianaceae) in the Tropical Andes
Authors: Vieu Julien C. , Koubínová Darina , Grant Jason R.
Published: August 2021

The evolutionary processes responsible for the extraordinary diversity in the middle elevation montane forests of the Tropical Andes (MMF; 1000–3500 m) remain poorly understood. It is not clear whether adaptive divergence, niche conservatism or geographical processes were the main contributors to the radiation of the respective lineages occurring there. We investigated the evolutionary history of plant lineages in the MMF. We used the vascular plant genus Macrocarpaea (Gentianaceae) as a model, as it consists of 118 morphologically diverse species, a majority of which are endemic to the MMF. We used a time-calibrated molecular phylogeny and morphological and climatic data to compare a set of evolutionary scenarios of various levels of complexity in a phylogenetic comparative framework. In this paper, we show that the hypothesis of adaptive radiation for Macrocarpaea in the MMF is unlikely. The genus remained confined to the upper montane forests (UMF > 1800 m) during more than a half of its evolutionary history, possibly due to evolutionary constraints. Later, coinciding with the beginning of the Pleistocene (around 2.58 Ma), a phylogenetically derived (recently branching) clade, here referred to as the M. micrantha clade (25 species), successfully colonized and radiated in the lower montane forests (LMF < 1800 m). This colonization was accompanied by the evolution of a new leaf phenotype that is unique to the species of the M. micrantha clade that likely represents an adaptation to life in this new environment (adaptive zone). Therefore, our results suggest that niche conservatism and geographical processes have dominated most of the diversification history of Macrocarpaea, but that a rare adaptive divergence event allowed a transition into a new adaptive zone and enabled progressive radiation in this zone through geographical processes.
Involvement of Tricarboxylic Acid Cycle Metabolites in Kidney Diseases
Authors: Jiménez-Uribe Alexis Paulina , Hernández-Cruz Estefani Yaquelin , Ramírez-Magaña Karla Jaqueline , Pedraza-Chaverri José
Published: August 2021

Mitochondria are complex organelles that orchestrate several functions in the cell. The primary function recognized is energy production; however, other functions involve the communication with the rest of the cell through reactive oxygen species (ROS), calcium influx, mitochondrial DNA (mtDNA), adenosine triphosphate (ATP) levels, cytochrome c release, and also through tricarboxylic acid (TCA) metabolites. Kidney function highly depends on mitochondria; hence mitochondrial dysfunction is associated with kidney diseases. In addition to oxidative phosphorylation impairment, other mitochondrial abnormalities have been described in kidney diseases, such as induction of mitophagy, intrinsic pathway of apoptosis, and releasing molecules to communicate to the rest of the cell. The TCA cycle is a metabolic pathway whose primary function is to generate electrons to feed the electron transport system (ETS) to drives energy production. However, TCA cycle metabolites can also release from mitochondria or produced in the cytosol to exert different functions and modify cell behavior. Here we review the involvement of some of the functions of TCA metabolites in kidney diseases.
Thromboelastometric Analysis of Anticancer Cerrena unicolor Subfractions Reveal Their Potential as Fibrin Glue Drug Carrier Enhancers
Authors: Stefaniuk Dawid , Misztal Tomasz , Pięt Mateusz , Zając Adrian , Kopycińska Magdalena , Matuszewska Anna , Ruminowicz-Stefaniuk Marta , Matuszewski Łukasz , Marcińczyk Natalia , Belcarz Anna , Żuchowski Jerzy , Skrabalak Ilona , Grąz Marcin , Ciołek Beata , Paduch Roman , Jaszek Magdalena
Published: August 2021

In this study, the influence of two subfractions (with previously proven anti-cancer properties) isolated from wood rot fungus Cerrena unicolor on the formation of a fibrin clot was investigated in the context of potential use as fibrin glue and sealant enhancers and potential wound healing agents. With the use of ROTEM thromboelastometry, we demonstrated that, in the presence of fibrinogen and thrombin, the S6 fraction accelerated the formation of a fibrin clot, had a positive effect on its elasticity modulus, and enhanced the degree of fibrin cross-linking. The S5 fraction alone showed no influence on the fibrin coagulation process; however, in the presence of fibrin, it exhibited a decrease in anti-proliferative properties against the HT-29 line, while it increased the proliferation of cells in general at a concentration of 100 µg/mL. Both fractions retained their proapoptotic properties to a lesser degree. In combination with the S6 fraction in the ratio of 1:1 and 1:3, the fractions contributed to increased inhibition of the activity of matrix metalloproteinases (MMPs). This may suggest anti-metastatic activity of the combined fractions. In conclusion, the potential of the fractions isolated from the C. unicolor secretome to be used as a means of improving the wound healing process was presented. The potential for delivering agents with cytostatic properties introduced far from the site of action or exerting a pro-proliferative effect at the wound site with the aid of a fibrin sealant was demonstrated.
Attenuation of PITPNM1 Signaling Cascade Can Inhibit Breast Cancer Progression
Authors: Liu Zihao , Shi Yu , Lin Qun , Yang Wenqian , Luo Qing , Cen Yinghuan , Li Juanmei , Fang Xiaolin , Jiang Wen G. , Gong Chang
Published: August 2021

Phosphatidylinositol transfer protein membrane-associated 1 (PITPNM1) contains a highly conserved phosphatidylinositol transfer domain which is involved in phosphoinositide trafficking and signaling transduction under physiological conditions. However, the functional role of PITPNM1 in cancer progression remains unknown. Here, by integrating datasets of The Cancer Genome Atlas (TCGA) and Molecular Taxonomy of Breast Cancer (METABRIC), we found that the expression of PITPNM1 is much higher in breast cancer tissues than in normal breast tissues, and a high expression of PITPNM1 predicts a poor prognosis for breast cancer patients. Through gene set variation analysis (GSEA) and gene ontology (GO) analysis, we found PITPNM1 is mainly associated with carcinogenesis and cell-to-cell signaling ontology. Silencing of PITPNM1, in vitro, significantly abrogates proliferation and colony formation of breast cancer cells. Collectively, PITPNM1 is an important prognostic indicator and a potential therapeutic target for breast cancer.
Colorimetric Point-of-Care Detection of Clostridium tyrobutyricum Spores in Milk Samples
Authors: Cecere Paola , Gatto Francesca , Cortimiglia Claudia , Bassi Daniela , Lucchini Franco , Cocconcelli Pier Sandro , Pompa Pier Paolo
Published: August 2021

Clostridium tyrobutyricum represents the main spoiling agent responsible for late blowing defects (LBD) in hard and semi-hard cheeses. Its spores are resistant to manufacturing procedures and can germinate during the long ripening process, causing the burst of the cheese paste with a consequent undesirable taste. The lower quality of blown cheeses leads to considerable financial losses for the producers. The early identification of spore contaminations in raw milk samples thus assumes a pivotal role in industrial quality control. Herein, we developed a point of care (POC) testing method for the sensitive detection of C. tyrobutyricum in milk samples, combining fast DNA extraction (with no purification steps) with a robust colorimetric loop-mediated isothermal amplification (LAMP) technique. Our approach allows for the sensitive and specific detection of C. tyrobutyricum spores (limit of detection, LoD: ~2 spores/mL), with the advantage of a clear naked-eye visualization of the results and a potential semi-quantitative discrimination of the contamination level. In addition, we demonstrated the feasibility of this strategy using a portable battery-operated device that allowed both DNA extraction and amplification steps, proving its potential for on-site quality control applications without the requirement of sophisticated instrumentation and trained personnel.
The Effect of Forward Testing as a Function of Test Occasions and Study Material
Authors: Sohlberg Robin , Olsson Fredrik , Gander Pierre
Published: August 2021

It has long been known that one of the most effective study techniques is to be tested on the to-be-remembered material, a phenomenon known as the testing effect. Recent research has also shown that testing of previous materials promotes the learning of new materials, a phenomenon known as the forward testing effect. In this paper, as of yet unexplored aspects of the forward testing effect related to face-name learning are examined; continuous and initial testing are compared to restudying, the effects of an initial test on subsequent learning, and whether an initial change of domain (change from one topic to another) regarding study material affects the robustness of the effect. An experiment (N = 94) was performed according to a 2 (Material: word pairs/face-name pairs in Block 1) × 3 (Test occasions: Blocks 1–4/Blocks 1 and 4/Block 4) complex between-groups design. The results showed that no difference between testing and repetition could be observed regarding the recall of faces and names. The restudy groups incorrectly recalled more names from previous lists in the last interim test compared to the tested groups, which supports the theory that interim tests reduce proactive interference. The results also suggest that the number of test occasions correlates with the number of incorrect recalls from previous lists. These results, in contrast to previous studies, highlight a potential uncertainty about the forward testing effect linked to the robustness of the phenomenon, the specificity in execution, and generalizability.
Using Appreciative Inquiry to Explore Effective Medical Interviews
Authors: Khawaja Masud
Published: August 2021

The objective of this study was to uncover the elements of successful medical interviews so that they can be easily shared with health educators, learners, and practitioners. The medical interview is still considered the most effective diagnostic tool available to physicians today, despite decades of rapid advancements in medical technology. When the physician-patient interaction is successful, outcomes are improved. Semi-structured interviews were conducted using an Appreciative Inquiry approach, which seeks to uncover strengths from positive experiences. The inquiry sought to identify the elements that comprise the participating physicians’ most successful patient interviews. Subsequent qualitative analysis revealed eight themes: social support, mutual respect, trust, active listening, relationships, nonverbal cues, empathy, and confidentiality. These themes do not each exist separately or in a vacuum from one another; they are in fact strongly interconnected and equally important. For instance, if a physician and a patient cannot at least maintain mutual respect, then building a relationship, or even trust, is impossible. Given the qualitative nature of this study, future quantitative research should seek to validate the results. As patients assume a more participatory role in modern medical encounters, communication and other soft skills will be key in satisfying patients and improving their medical outcomes.
Insights into Public Perceptions of Earthship Buildings as Alternative Homes
Authors: Booth Colin A. , Rasheed Sona , Mahamadu Abdul-Majeed , Horry Rosemary , Manu Patrick , Awuah Kwasi Gyau Baffour , Aboagye-Nimo Emmanuel , Georgakis Panagiotis
Published: August 2021

Sustainable futures necessitate a concomitant requirement for both sustainable buildings and sustainable behaviours under one roof. The defining principles behind Earthship buildings are to promote the use of local, recycled, waste, natural and renewable materials in their construction, for the adoption of a passive solar design for internal heating/cooling, collection of rainwater as a potable water supply, and encourage the onsite recycling of used water for plants to aid food production. However, despite growth in Earthship buildings constructed across many countries of the world, their appeal has not yet made a noticeable contribution to mainstream housing. Therefore, this study is the first to attempt to explore public perceptions towards the benefits and barriers of Earthship buildings as a means of understanding their demand by potential home builders/owners. Opinions were sought through questionnaire surveys completed by visitors to the Brighton Earthship building. Results reveal that the public believe that the reclamation of rainwater and greywater, renewable energy consumption and use of recycled materials included in the design/build are the major benefits of Earthship buildings, whilst the opportunity for a modern living style in a conservative lifestyle/setting, having a building that is cheaper than an ordinary home and the possibility of living totally off grid are considered the least beneficial reasons for building Earthship homes. Results also reveal that the public believe acquiring necessary permits/permissions to build may be more complicated, securing financial support (mortgage/loan) may be more challenging, and identifying/attaining suitable building plots are major barriers of Earthship buildings, whilst the futuristic/alternative building design, being built from waste materials and being entirely dependent on renewable resources (rainfall/wind/sunshine) are considered the least important barriers to building Earthship homes. Notwithstanding the participants included in this study already having an interest in Earthship buildings/lifestyles, it is concluded that the general public deem the general principles of Earthships as an acceptable choice of building/living but it is the formal means of building or buying an Earthship home that is the greatest hurdle against the uptake of Earthship buildings. Therefore, if sustainable futures are to be realized, it is proposed that a shift away from traditional house building towards Earthship building will require the involvement of all stakeholders immersed in the building process (architects, planners, builders, investors, lawyers) to path an easier journey for Earthship buildings and sustainable living.
Ultrasound Assisted Synthesis of Gadolinium Oxide-Zeolitic Imidazolate Framework-8 Nanocomposites and Their Optimization for Photocatalytic Degradation of Methyl Orange Using Response Surface Methodology
Authors: Jeon Su-Gyeong , Ko Jeong-Won , Ko Weon-Bae
Published: August 2021

An ultrasound-assisted method was used to prepare gadolinium oxide (Gd2O3)-zeolitic imidazolate framework (ZIF)-8 nanocomposites. The surface morphology, particle size, and properties of the Gd2O3-ZIF-8 nanocomposites were examined using scanning electron microscopy (SEM), X-ray diffraction (XRD), Raman spectroscopy, and ultraviolet-visible (UV-vis) spectroscopy. The synthesized Gd2O3-ZIF-8 nanocomposites were used as a catalyst to degrade methyl orange (MO) under UV light irradiation at 254 nm. The color of the aqueous MO dye solution during photocatalytic degradation was examined using color spectroscopy. Response surface methodology (RSM) using a four-factor Box-Behnken design (BBD) was used to design the experiments and optimize the photocatalytic degradation of MO. The significance of the experimental factors and their interactions were determined using analysis of variance (ANOVA). The efficiency of Gd2O3-ZIF-8 nanocomposites for the photocatalytic degradation of MO reached 98.05% within 40 min under UV irradiation at 254 nm under the experimental conditions of pH 3.3, 0.4 g/L catalyst dose, 0.0630 mM MO concentration, and 431.79 mg/L H2O2 concentration. The kinetics study showed that the MO photocatalytic degradation followed a pseudo-first-order reaction rate law.
Enhanced Photocatalytic Activity of Hybrid rGO@TiO2/CN Nanocomposite for Organic Pollutant Degradation under Solar Light Irradiation
Authors: Kocijan Martina , Ćurković Lidija , Radošević Tina , Podlogar Matejka
Published: August 2021

The three-component hybrid (rGO/TiO2/CN) nanocomposite was prepared in order to enhance the photocatalytic properties of anatase TiO2 nanoparticles (NPs) under solar-like irradiation. The rGO/TiO2/CN was prepared in a mixture of the reduced graphene oxide (rGO, 8 wt%), anatase TiO2 nanoparticles (NPs), and graphitic carbon nitride (g-C3N4, 16 wt%). It was self-assembled through the one-step hydrothermal method, followed by an annealing process. The photocatalyst was thoroughly characterized by means of X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), transmission electron microscopy (TEM), the Brunauer-Emmett-Teller (BET) nitrogen adsorption/desorption technique and UV-Vis diffuse reflectance spectroscopy (DRS). The photocatalytic activity of the TiO2, TiO2/rGO, TiO2/CN and hybrid rGO/TiO2/CN nanocomposite was studied through the degradation of a rhodamine B (RhB) aqueous solution under solar-like irradiation. The results showed that the highest photocatalytic activity was achieved by the rGO/TiO2/CN mixture, which can be attributed to the synergistic effect of the incorporation of both rGO and g-C3N4 with TiO2. Further, the influence of the pH value of the RhB dye aqueous solution and different water matrix (Milli-Q, tap, and alkaline water) on the photocatalytic efficiency of the rGO/TiO2/CN nanocomposite was examined. In addition, a recycle test was performed for hybrid rGO@TiO2/CN to investigate the effectiveness of the photodegradation of RhB dye in three successive cycles. The conducted results indicate that the pH value of RhB dye aqueous solution and water matrices play an important role in the photocatalytic degradation rate.
Sphingolipids: Effectors and Achilles Heals in Viral Infections?
Authors: Schneider-Schaulies Sibylle , Schumacher Fabian , Wigger Dominik , Schöl Marie , Waghmare Trushnal , Schlegel Jan , Seibel Jürgen , Kleuser Burkhard
Published: August 2021

As viruses are obligatory intracellular parasites, any step during their life cycle strictly depends on successful interaction with their particular host cells. In particular, their interaction with cellular membranes is of crucial importance for most steps in the viral replication cycle. Such interactions are initiated by uptake of viral particles and subsequent trafficking to intracellular compartments to access their replication compartments which provide a spatially confined environment concentrating viral and cellular components, and subsequently, employ cellular membranes for assembly and exit of viral progeny. The ability of viruses to actively modulate lipid composition such as sphingolipids (SLs) is essential for successful completion of the viral life cycle. In addition to their structural and biophysical properties of cellular membranes, some sphingolipid (SL) species are bioactive and as such, take part in cellular signaling processes involved in regulating viral replication. It is especially due to the progress made in tools to study accumulation and dynamics of SLs, which visualize their compartmentalization and identify interaction partners at a cellular level, as well as the availability of genetic knockout systems, that the role of particular SL species in the viral replication process can be analyzed and, most importantly, be explored as targets for therapeutic intervention.
A Universal Approach to Analyzing Transmission Electron Microscopy with ImageJ
Authors: Lam Jacob , Katti Prasanna , Biete Michelle , Mungai Margaret , AshShareef Salma , Neikirk Kit , Garza Lopez Edgar , Vue Zer , Christensen Trace A. , Beasley Heather K. , Rodman Taylor A. , Murray Sandra A. , Salisbury Jeffrey L. , Glancy Brian , Shao Jianqiang , Pereira Renata O. , Abel E. Dale , Hinton Antentor
Published: August 2021

Transmission electron microscopy (TEM) is widely used as an imaging modality to provide high-resolution details of subcellular components within cells and tissues. Mitochondria and endoplasmic reticulum (ER) are organelles of particular interest to those investigating metabolic disorders. A straightforward method for quantifying and characterizing particular aspects of these organelles would be a useful tool. In this protocol, we outline how to accurately assess the morphology of these important subcellular structures using open source software ImageJ, originally developed by the National Institutes of Health (NIH). Specifically, we detail how to obtain mitochondrial length, width, area, and circularity, in addition to assessing cristae morphology and measuring mito/endoplasmic reticulum (ER) interactions. These procedures provide useful tools for quantifying and characterizing key features of sub-cellular morphology, leading to accurate and reproducible measurements and visualizations of mitochondria and ER.
The Multifaceted Regulation of SnRK2 Kinases
Authors: Maszkowska Justyna , Szymańska Katarzyna Patrycja , Kasztelan Adrian , Krzywińska Ewa , Sztatelman Olga , Dobrowolska Grażyna
Published: August 2021

SNF1-related kinases 2 (SnRK2s) are central regulators of plant responses to environmental cues simultaneously playing a pivotal role in the plant development and growth in favorable conditions. They are activated in response to osmotic stress and some of them also to abscisic acid (ABA), the latter being key in ABA signaling. The SnRK2s can be viewed as molecular switches between growth and stress response; therefore, their activity is tightly regulated; needed only for a short time to trigger the response, it has to be induced transiently and otherwise kept at a very low level. This implies a strict and multifaceted control of SnRK2s in plant cells. Despite emerging new information concerning the regulation of SnRK2s, especially those involved in ABA signaling, a lot remains to be uncovered, the regulation of SnRK2s in an ABA-independent manner being particularly understudied. Here, we present an overview of available data, discuss some controversial issues, and provide our perspective on SnRK2 regulation.
Proteomic Analysis of Estrogen-Mediated Enhancement of Mesenchymal Stem Cell-Induced Angiogenesis In Vivo
Authors: Mihai Maria Cristina , Popa Mirel Adrian , Șuică Viorel Iulian , Antohe Felicia , Jackson Edwin K. , Leeners Brigitte , Simionescu Maya , Dubey Raghvendra K.
Published: August 2021

Therapeutic use of mesenchymal stem cells (MSCs) for tissue repair has great potential. MSCs from multiple sources, including those derived from human umbilical matrix, namely Wharton’s jelly, may serve as a resource for obtaining MSCs. However, low in vivo engraftment efficacy of MSCs remains a challenging limitation. To improve clinical outcomes using MSCs, an in-depth understanding of the mechanisms and factors involved in successful engraftment is required. We recently demonstrated that 17β-estradiol (E2) improves MSCs in vitro proliferation, directed migration and engraftment in murine heart slices. Here, using a proteomics approach, we investigated the angiogenic potential of MSCs in vivo and the modulatory actions of E2 on mechanisms involved in tissue repair. Specifically, using a Matrigel® plug assay, we evaluated the effects of E2 on MSCs-induced angiogenesis in ovariectomized (OVX) mice. Moreover, using proteomics we investigated the potential pro-repair processes, pathways, and co-mechanisms possibly modified by the treatment of MSCs with E2. Using RT-qPCR, we evaluated mRNA expression of pro-angiogenic molecules, including endoglin, Tie-2, ANG, and VEGF. Hemoglobin levels, a marker for blood vessel formation, were increased in plugs treated with E2 + MSCs, suggesting increased capillary formation. This conclusion was confirmed by the histological analysis of capillary numbers in the Matrigel® plugs treated with E2 + MSC. The LC-MS screening of proteins obtained from the excised Matrigel® plugs revealed 71 proteins that were significantly altered following E2 exposure, 57 up-regulated proteins and 14 down-regulated proteins. A major result was the association of over 100 microRNA molecules (miRNAs) involved in cellular communication, vesicle transport, and metabolic and energy processes, and the high percentage of approximately 25% of genes involved in unknown biological processes. Together, these data provide evidence for increased angiogenesis by MSCs treated with the sex hormone E2. In conclusion, E2 treatment may increase the engraftment and repair potential of MSCs into tissue, and may promote MSC-induced angiogenesis after tissue injury.
Ultrasonic Dyeing of Polyester Fabric with Azo Disperse Dyes Clubbed with Pyridonones and Its UV Protection Performance
Authors: Al-Etaibi Alya M. , El-Apasery Morsy Ahmed
Published: August 2021

The textile sector is closely linked to environmental pollution as a result of the use of toxic chemicals and their disposal in liquid waste, which negatively affects for the environment. Moreover, textile industries, especially wet processing, consume a large amount of energy, water, and chemical auxiliaries. Therefore, there is an urgent need to find a solution that takes the problem of environmental pollution into account. Considering ultrasound as an environmentally safe alternative for dyeing polyester fabrics with the disperse dyes that we have prepared before, the comparison between the ultrasonic dyeing method and conventional dyeing at low temperatures was investigated. Dye exhaustion on polyester fabrics and fastness properties such as the washing, rubbing, light, and perspiration of all of the dyed fabrics were performed by two dyeing methods. Additionally, the ultraviolet protection factors (UPF) for dyed polyester fabrics were evaluated.