bluuebunny/crossref_metadata_2025 huggingface dataset:
The Combined Impact of Female and Male Body Mass Index on Cumulative Pregnancy Outcomes After the First Ovarian Stimulation
Authors: Zhao Zhonghua , Jiang Xue , Li Jing , Zhang Menghui , Liu Jinhao , Dai Shanjun , Shi Hao , Liang Yuling , Yang Li , Guo Yihong
Published: September 2021

Objectives

To evaluate the combined impact of male and female BMI on cumulative pregnancy outcomes after the first ovarian stimulation.

Design

Retrospective cohort study.

Setting

University-affiliated reproductive medicine center.

Patients

A total of 15,972 couples undergoing their first ovarian stimulations from June 2009 to June 2016 were included. During the follow-up period between June 2009 and June 2018, 14,182 couples underwent a complete ART cycle involving fresh embryo transfer and subsequent frozen embryo transfers (FETs) after their first ovarian stimulations. Patients with a BMI <24 kg/m2 served as the reference group. Patients with a BMI ≥ 24 kg/m2 were considered to be overweight, and those with a BMI ≥28 kg/m2 were considered to be obese.

Intervention(s)

None.

Primary Outcome Measure

The primary outcome was the cumulative live birth rate (CLBR), which defined as the delivery of at least one live birth in the fresh or in the subsequent FET cycles after the first ovarian stimulation.

Results

In the analyses of females and males separately, compared with the reference group, overweight and obese females had a reduced CLBR (aOR 0.83, 95% CI 0.7.92 and aOR 0.76, 95% CI 0.64–0.90). Similarly, overweight males had a reduced CLBR (aOR 0.91, 95% CI 0.83–0.99) compared with that of the reference group. In the analyses of couples, those in which the male was in the reference or overweight group and the female was overweight or obese had a significantly lower CLBR than those in which both the male and female had a BMI <24 kg/m2.

Conclusions

The CLBR is negatively impacted by increased BMI in the female and overweight status in the male, both individually and together.
Efficiency of Air Purifiers at Removing Air Pollutants in Educational Facilities: A Preliminary Study
Authors: Brągoszewska Ewa , Biedroń Izabela
Published: September 2021

Infectious diseases caused by airborne bacteria and viruses are a major problem for both social and economic reasons. The significance of this phenomenon is particularly noticeable during the time of the coronavirus pandemic. One of the consequences is the increased interest in the air purifier (AP) market, which resulted in a significant increase in sales of these devices. In this study, we tested the efficiency of APs in removing bacterial air contamination in the educational context in the Upper Silesia region of Poland during the “cold season” of 2018/2019. During the 6 months of measuring microbiological air quality, an 18% decrease in the concentration of microbiological pollutants as a result of the action of the APs was recorded. Additionally, the results of the particle size distribution of the bacterial aerosols showed a reduction in the share of the respirable fraction (particles with an aerodynamic diameter below 3.3 µm) by an average of 20%. The dominance of gram-positive cocci in the indoor environment indicates that humans are the main source of most of the bacteria present in the building. We conclude that the use of APs may significantly decrease the level of concentration of microbiological air pollutants and reduce the negative health effects of indoor bioaerosols; however, further work that documents this phenomenon is needed.
Modelling the Efficacy of Febrile Heating in Infected Endotherms
Authors: Lewis Gregory , Bonsall Michael B.
Published: September 2021

Fever is a response to infection characterised by an increase in body temperature. The adaptive value of this body temperature increase for endotherms is unclear, given the relatively small absolute temperature increases associated with endotherm fever, its substantial metabolic costs, and the plausibility for pathogens to adapt to higher temperatures. We consider three thermal mechanisms for fever's antimicrobial effect: (1) direct growth inhibition by elevating temperature above the pathogens optimal growth temperature; (2) further differentiating the host body from the wider environment; and (3) through increasing thermal instability of the pathogen environment. We assess these by modelling their effects pathogen on temperature dependent growth, finding thermal effects can vary from highly to minimally effective depending on pathogen species. We also find, depending on the specification of a simple physical model, intermittent heating can inhibit pathogen growth more effectively than continuous heating with an energy constraint.
Effects of Soil Temperature and Moisture on the Development and Survival of Grasshopper Eggs in Inner Mongolian Grasslands
Authors: Wu Tingjuan , Hao Shuguang , Kang Le
Published: September 2021

Grasshopper eggs overwinter in soil for almost half a year. Changes in soil temperature and moisture have a substantial effect on grasshopper eggs, especially temperature and moisture extremes. However, the combinatorial effect of temperature and moisture on the development and survival of grasshopper eggs has not been well studied. Here, we examined the effects of different soil moistures (2, 5, 8, 11, 14% water content) at 26°C and combinations of extreme soil moisture and soil temperature on the egg development and survival of three dominant species of grasshopper (Dasyhippus barbipes, Oedaleus asiaticus, and Chorthippus fallax) in Inner Mongolian grasslands. Our data indicated that the egg water content of the three grasshopper species was positively correlated with soil moisture but negatively correlated with hatching time. The relationship between hatching rate and soil moisture was unimodal. Averaged across 2 and 11% soil moisture, a soil temperature of 35oCsignificantly advanced the egg hatching time of D. barbipes, O. asiaticus, and C. fallax by 5.63, 4.75, and 2.63 days and reduced the egg hatching rate of D. barbipes by 18%. Averaged across 26 and 35°C, 2% soil moisture significantly delayed the egg hatching time of D. barbipes, O. asiaticus, and C. fallax by 0.69, 11.01, and 0.31 days, respectively, and decreased the egg hatching rate of D. barbipes by 10%. The hatching time was prolonged as drought exposure duration increased, and the egg hatching rate was negatively correlated with drought exposure duration, except for O. asiaticus. Overall, the combination of high soil temperature and low soil moisture had a significantly negative effect on egg development, survival, and egg hatching. Generally, the response of grasshopper eggs to soil temperature and moisture provides important information on the population dynamics of grasshoppers and their ability to respond to future climate change.
An Environmental Flow Framework for Riverine Macroinvertebrates During Dry and Wet Seasons Through Non-linear Ecological Modeling
Authors: Luo Qingyi , Chiu Ming-Chih , Tan Lu , Cai Qinghua
Published: September 2021

A suitable environmental flow is critical for the functional maintenance of riverine ecosystems. Hydropower plants alter the flow regime by decreasing or even drying up the streamflow downstream of the dams, thereby affecting ecosystem sustainability. In this study, we aimed to develop a robust environmental flow framework that can provide scientific evidence for sustainable water resource management. Using ecological niche modeling based on non-linear responses of species to habitat factors, we assessed the environmental flow in the Xiangxi River Basin of Central China during dry and wet seasons from a multi-year perspective. The most abundant macroinvertebrate taxon (i.e.,Baetis) was selected for model testing. The results showed seasonal differences in the minimum ecological water requirements and optimal environmental flow. These two hydrological metrics were higher during the wet season than during the dry season. During the dry season, the minimum ecological water requirement ofBaetiswas 1.3 m3·s−1, and the optimal environmental flow was 1.6 m3·s−1. During the wet season, the minimum ecological water requirement ofBaetiswas 2.5 m3·s−1, and the optimal environmental flow was 2.6 m3·s−1. This study provides a theoretical basis for the robust management of water resources in river basins.
Common Patterns of Skull Bone Fusion and Their Potential to Discriminate Different Ontogenetic Stages in Extant Birds
Authors: Plateau Olivia , Foth Christian
Published: September 2021

The degree of sutural closure between bones generally allows for the classification of skeleton maturity in tetrapods. In mammals, the sutural closure of skull bones was previously used as proxy to evaluate the ontogenetic stage of single individuals. However, due to temporal variation, this process can be only applied among mammalian subclades, but not for all mammals in general. In contrast, the process of sutural closures in bird skulls could be a more reliable ontogenetic proxy for this clade as adult birds commonly show a generally high degree of bone fusion. To test this, we studied the process of sutural closure in ontogenetic series of 18 extant bird species regarding the presence of an ontogenetic signal and compared the results with changes in skull size and proportions. Univariate analyses indicate that bone fusion happens faster in altricial than in precocial birds. However, the use of PCoA and multivariate regressions reveal that the skull bone fusion follows a common pattern among birds and thus can be used as proxy to identify different ontogenetic stages. In general, the process of sutural closure spreads from posterior to anterior and from ventral to dorsal. In contrast, skull measurements reflect rather interspecific allometry than ontogeny. The used of bone fusion as proxy will help to better identify and compare different stages of maturation in birds, including historical material from osteological collections.
A Genetically Informed Longitudinal Study of Loneliness and Dementia Risk in Older Adults
Authors: Kim Alice J. , Gold Alaina I. , Fenton Laura , Pilgrim Matthew J. D. , Lynch Morgan , Climer Cailin R. , Penichet Eric N. , Kam Alyssa , Beam Christopher R.
Published: September 2021

Although several studies have shown small longitudinal associations between baseline loneliness and subsequent dementia risk, studies rarely test whether change in loneliness predicts dementia risk. Furthermore, as both increase with advancing age, genetic and environmental selection processes may confound the putative causal association between loneliness and dementia risk. We used a sample of 2,476 individual twins from three longitudinal twin studies of aging in the Swedish Twin Registry to test the hypothesis that greater positive change in loneliness predicts greater dementia risk. We then used a sample of 1,632 pairs of twins to evaluate the hypothesis that effects of change in loneliness on dementia risk would remain after adjusting for effects of genetic and environmental variance. Phenotypic model results suggest that mild levels of baseline loneliness predict greater dementia risk. Contrary to our hypothesis, change in loneliness did not correlate with dementia risk, regardless of whether genetic and environmental selection confounds were taken into account. Worsening loneliness with age may not confer greater dementia risk.
Identification of Transcription Factor-Related Gene Signature and Risk Score Model for Colon Adenocarcinoma
Authors: Lin Jianwei , Cao Zichao , Yu Dingye , Cai Wei
Published: September 2021

The prognosis of colon adenocarcinoma (COAD) remains poor. However, the specific and sensitive biomarkers for diagnosis and prognosis of COAD are absent. Transcription factors (TFs) are involved in many biological processes in cells. As the molecule of the signal pathway of the terminal effectors, TFs play important roles in tumorigenesis and development. A growing body of research suggests that aberrant TFs contribute to the development of COAD, as well as to its clinicopathological features and prognosis. In consequence, a few studies have investigated the relationship between the TF-related risk model and the prognosis of COAD. Therefore, in this article, we hope to develop a prognostic risk model based on TFs to predict the prognosis of patients with COAD. The mRNA transcription data and corresponding clinical data were downloaded from TCGA and GEO. Then, 141 differentially expressed genes, validated by the GEPIA2 database, were identified by differential expression analysis between normal and tumor samples. Univariate, multivariate and Lasso Cox regression analysis were performed to identify seven prognostic genes (E2F3, ETS2, HLF, HSF4, KLF4, MEIS2, and TCF7L1). The Kaplan–Meier curve and the receiver operating characteristic curve (ROC, 1-year AUC: 0.723, 3-year AUC: 0.775, 5-year AUC: 0.786) showed that our model could be used to predict the prognosis of patients with COAD. Multivariate Cox analysis also reported that the risk model is an independent prognostic factor of COAD. The external cohort (GSE17536 and GSE39582) was used to validate our risk model, which indicated that our risk model may be a reliable predictive model for COAD patients. Finally, based on the model and the clinicopathological factors, we constructed a nomogram with a C-index of 0.802. In conclusion, we emphasize the clinical significance of TFs in COAD and construct a prognostic model of TFs, which could provide a novel and reliable model for the prognosis of COAD.
A Role of Variance in Interferon Genes to Disease Severity in COVID-19 Patients
Authors: Gozman Leonid , Perry Kellie , Nikogosov Dimitri , Klabukov Ilya , Shevlyakov Artem , Baranova Ancha
Published: September 2021

The rapid rise and global consequences of the novel coronavirus disease 19 (COVID-19) have again brought the focus of the scientific community on the possible host factors involved in patient response and outcome to exposure to the virus. The disease severity remains highly unpredictable, and individuals with none of the aforementioned risk factors may still develop severe COVID-19. It was shown that genotype-related factors like an ABO Blood Group affect COVID-19 severity, and the risk of infection with SARS-CoV-2 was higher for patients with blood type A and lower for patients with blood type O. Currently it is not clear which specific genes are associated with COVID-19 severity. The comparative analysis of COVID-19 and other viral infections allows us to predict that the variants within the interferon pathway genes may serve as markers of the magnitude of immune response to specific pathogens. In particular, various members of Class III interferons (lambda) are reviewed in detail.
Recent Advances in Liquid Biopsy of Brain Cancers
Authors: An Yunyun , Fan Fei , Jiang Xiaobing , Sun Kun
Published: September 2021

Brain cancers are among the top causes of death worldwide. Although, the survival rates vary widely depending on the type of the tumor, early diagnosis could generally benefit in better prognosis outcomes of the brain cancer patients. Conventionally, neuroimaging and biopsy are the most widely used approaches in diagnosis, subtyping, and prognosis monitoring of brain cancers, while emerging liquid biopsy assays using peripheral blood or cerebrospinal fluid have demonstrated many favorable characteristics in this task, especially due to their minimally invasive and easiness in sampling nature. Here, we review the recent studies in the liquid biopsy of brain cancers. We discuss the methodologies and performances of various assays on diagnosis, tumor subtyping, relapse prediction as well as prognosis monitoring in brain cancers, which approaches have made a big step toward clinical benefits of brain cancer patients.
Genome-Wide Identification and Analysis of the NF-Y Gene Family in Potato (Solanum tuberosum L.)
Authors: Liu Zhen , Li Yuanming , Zhu Jinyong , Ma Wenjing , Li Zhitao , Bi Zhenzhen , Sun Chao , Bai Jiangping , Zhang Junlian , Liu Yuhui
Published: September 2021

Nuclear factor Y (NF-Y) is a ubiquitous transcription factor in eukaryotes, which is composed of three subunits (NF-YA, NF-YB, and NF-YC). NF-Y has been identified as a key regulator of multiple pathways in plants. Although the NF-Y gene family has been identified in many plants, it has not been reported in potato (Solanum tuberosum). In the present study, a total of 41 NF-Y proteins in potato (StNF-Ys) were identified, including 10 StNF-YA, 22 StNF-YB, and nine StNF-YC subunits, and their distribution on chromosomes, gene structure, and conserved motif was analyzed. A synteny analysis indicated that 14 and 38 pairs of StNF-Y genes were orthologous to Arabidopsis and tomato (Solanum lycopersicum), respectively, and these gene pairs evolved under strong purifying selection. In addition, we analyzed the expression profiles of NF-Y genes in different tissues of double haploid (DM) potato, as well as under abiotic stresses and hormone treatments by RNA-seq downloaded from the Potato Genome Sequencing Consortium (PGSC) database. Furthermore, we performed RNA-seq on white, red, and purple tuber skin and flesh of three potato cultivars at the tuber maturation stage to identify genes that might be involved in anthocyanin biosynthesis. These results provide valuable information for improved understanding of StNF-Y gene family and further functional analysis of StNF-Y genes in fruit development, abiotic stress tolerance, and anthocyanin biosynthesis in potato.
Proteomic Response of Rat Pituitary Under Chronic Mild Stress Reveals Insights Into Vulnerability and Resistance to Anxiety or Depression
Authors: Tian Fenfang , Liu Dan , Chen Jin , Liao Wei , Gong Weibo , Huang Rongzhong , Xie Liang , Yi Faping , Zhou Jian
Published: September 2021

Chronic stress as one of the most significant risk factor can trigger overactivity of hypothalamic-pituitary-adrenal (HPA) axis in depression as well as anxiety. Yet, the shared and unique neurobiological underpinnings underlying the pituitary abnormality in these two disorders have not been made clear. We previously have established depression-susceptible, anxiety-susceptible and insusceptible groups using a valid chronic mild stress (CMS) model. In this work, the possible protein expression changes in the rat pituitary of these three groups were continuously investigated through the use of the comparative quantitative proteomics and bioinformatics approaches. The pituitary-proteome analysis identified totally 197 differential proteins as a CMS response. These deregulated proteins were involved in diverse biological functions and significant pathways potentially connected with the three different behavioral phenotypes, likely serving as new investigative protein targets. Afterwards, parallel reaction monitoring-based independent analysis found out that expression alterations in Oxct1, Sec24c, Ppp1cb, Dock1, and Coq3; Lama1, Glb1, Gapdh, Sccpdh, and Renbp; Sephs1, Nup188, Spp1, Prodh1, and Srm were specifically linked to depression-susceptible, anxiety-susceptible and insusceptible groups, respectively, suggesting that the same CMS had different impacts on the pituitary protein regulatory system. Collectively, the current proteomics research elucidated an important molecular basis and furnished new valuable insights into neurochemical commonalities and specificities of the pituitary dysfunctional mechanisms in HPA axis underlying vulnerability and resistance to stress-induced anxiety or depression.
Long-Term Elevated Inflammatory Protein Levels in Asymptomatic SARS-CoV-2 Infected Individuals
Authors: Tserel Liina , Jõgi Piia , Naaber Paul , Maslovskaja Julia , Häling Annika , Salumets Ahto , Zusinaite Eva , Soeorg Hiie , Lättekivi Freddy , Ingerainen Diana , Soots Mari , Toompere Karolin , Kaarna Katrin , Kisand Kai , Lutsar Irja , Peterson Pärt
Published: September 2021

The clinical features of SARS-CoV-2 infection range from asymptomatic to severe disease with life-threatening complications. Understanding the persistence of immune responses in asymptomatic individuals merit special attention because of their importance in controlling the spread of the infections. We here studied the antibody and T cell responses, and a wide range of inflammation markers, in 56 SARS-CoV-2 antibody-positive individuals, identified by a population screen after the first wave of SARS-CoV-2 infection. These, mostly asymptomatic individuals, were reanalyzed 7-8 months after their infection together with 115 age-matched seronegative controls. We found that 7-8 months after the infection their antibodies to SARS-CoV-2 Nucleocapsid (N) protein declined whereas we found no decrease in the antibodies to Spike receptor-binding domain (S-RBD) when compared to the findings at seropositivity identification. In contrast to antibodies to N protein, the antibodies to S-RBD correlated with the viral neutralization capacity and with CD4+ T cell responses as measured by antigen-specific upregulation of CD137 and CD69 markers. Unexpectedly we found the asymptomatic antibody-positive individuals to have increased serum levels of S100A12, TGF-alpha, IL18, and OSM, the markers of activated macrophages-monocytes, suggesting long-term persistent inflammatory effect associated with the viral infection in asymptomatic individuals. Our results support the evidence for the long-term persistence of the inflammation process and the need for post-infection clinical monitoring of SARS-CoV-2 infected asymptomatic individuals.
Formyl Peptide Receptors and Annexin A1: Complementary Mechanisms to Infliximab in Murine Experimental Colitis and Crohn’s Disease
Authors: de Paula-Silva Marina , da Rocha Gustavo Henrique Oliveira , Broering Milena Fronza , Queiroz Maria Luíza , Sandri Silvana , Loiola Rodrigo Azevedo , Oliani Sonia Maria , Vieira Andrea , Perretti Mauro , Farsky Sandra Helena Poliselli
Published: September 2021

Non-responsiveness to anti-TNF-α therapies presents relevant rates in inflammatory bowel disease patients, presenting the need to find biomarkers involved in therapeutic efficacy. Herein, we demonstrate that higher levels of colonic formyl peptide receptor 1 and annexin A1 correlate with histological recovery in Crohn’s disease patients under remission. Using the dextran sulfate sodium colitis model in mice, we suggest that infliximab induces annexin A1 expression and secretion in activated intestinal leukocytes. Conversely, this mechanism might stimulate epithelial formyl peptide receptors, inducing wound healing and consequent histological remission. Our data indicate that assessing intestinal expressions of formyl peptide receptors and annexin A1 might provide precious information on the disease activity and responsiveness to infliximab in inflammatory bowel disease patients.
Construction of a Ferroptosis-Related Nine-lncRNA Signature for Predicting Prognosis and Immune Response in Hepatocellular Carcinoma
Authors: Xu Zhijie , Peng Bi , Liang Qiuju , Chen Xi , Cai Yuan , Zeng Shuangshuang , Gao Kewa , Wang Xiang , Yi Qiaoli , Gong Zhicheng , Yan Yuanliang
Published: September 2021

Ferroptosis is an iron-dependent cell death process that plays important regulatory roles in the occurrence and development of cancers, including hepatocellular carcinoma (HCC). Moreover, the molecular events surrounding aberrantly expressed long non-coding RNAs (lncRNAs) that drive HCC initiation and progression have attracted increasing attention. However, research on ferroptosis-related lncRNA prognostic signature in patients with HCC is still lacking. In this study, the association between differentially expressed lncRNAs and ferroptosis-related genes, in 374 HCC and 50 normal hepatic samples obtained from The Cancer Genome Atlas (TCGA), was evaluated using Pearson’s test, thereby identifying 24 ferroptosis-related differentially expressed lncRNAs. The least absolute shrinkage and selection operator (LASSO) algorithm and Cox regression model were used to construct and validate a prognostic risk score model from both TCGA training dataset and GEO testing dataset (GSE40144). A nine-lncRNA-based signature (CTD-2033A16.3, CTD-2116N20.1, CTD-2510F5.4, DDX11-AS1, LINC00942, LINC01224, LINC01231, LINC01508, and ZFPM2-AS1) was identified as the ferroptosis-related prognostic model for HCC, independent of multiple clinicopathological parameters. In addition, the HCC patients were divided into high-risk and low-risk groups according to the nine-lncRNA prognostic signature. The gene set enrichment analysis enrichment analysis revealed that the lncRNA-based signature might regulate the HCC immune microenvironment by interfering with tumor necrosis factor α/nuclear factor kappa-B, interleukin 2/signal transducers and activators of transcription 5, and cytokine/cytokine receptor signaling pathways. The infiltrating immune cell subtypes, such as resting memory CD4(+) T cells, follicular helper T cells, regulatory T cells, and M0 macrophages, were all significantly different between the high-risk group and the low-risk group as indicated in Spearman’s correlation analysis. Moreover, a substantial increase in the expression of B7H3 immune checkpoint molecule was found in the high-risk group. Our findings provided a promising insight into ferroptosis-related lncRNAs in HCC and a personalized prediction tool for prognosis and immune responses in patients.
Trained Immunity Confers Prolonged Protection From Listeriosis
Authors: Théroude Charlotte , Reverte Marta , Heinonen Tytti , Ciarlo Eleonora , Schrijver Irene T. , Antonakos Nikolaos , Maillard Nicolas , Pralong Florian , Le Roy Didier , Roger Thierry
Published: September 2021

Trained immunity refers to the ability of the innate immune system exposed to a first challenge to provide an enhanced response to a secondary homologous or heterologous challenge. We reported that training induced with β-glucan one week before infection confers protection against a broad-spectrum of lethal bacterial infections. Whether this protection persists over time is unknown. To tackle this question, we analyzed the immune status and the response to Listeria monocytogenes (L. monocytogenes) of mice trained 9 weeks before analysis. The induction of trained immunity increased bone marrow myelopoiesis and blood counts of Ly6Chigh inflammatory monocytes and polymorphonuclear neutrophils (PMNs). Ex vivo, whole blood, PMNs and monocytes from trained mice produced increased levels of cytokines in response to microbial products and limited the growth of L. monocytogenes. In vivo, following challenge with L. monocytogenes, peripheral blood leukocytes were massively depleted in control mice but largely preserved in trained mice. PMNs were reduced also in the spleen from control mice, and increased in the spleen of trained mice. In transwell experiments, PMNs from trained mice showed increased spontaneous migration and CXCL2/MIP2α-induced chemotaxis, suggesting that training promotes the migration of PMNs in peripheral organs targeted by L. monocytogenes. Trained PMNs and monocytes had higher glycolytic activity and mitochondrial respiration than control cells when exposed to L. monocytogenes. Bacterial burden and dissemination in blood, spleen and liver as well as systemic cytokines and inflammation (multiplex bead assay and bioluminescence imaging) were reduced in trained mice. In full agreement with these results, mice trained 9 weeks before infection were powerfully protected from lethal listeriosis. Altogether, these data suggest that training increases the generation and the antimicrobial activity of PMNs and monocytes, which may confer prolonged protection from lethal bacterial infection.
The Impact of Ageing on the CNS Immune Response in Alzheimer’s Disease
Authors: Chee Stephan En Jie , Solito Egle
Published: September 2021

Alzheimer’s Disease (AD) is a progressive neurodegenerative disease strongly associated with increasing age. Neuroinflammation and the accumulation of amyloid protein are amongst the hallmarks of this disease and most translational research to date has focused on targeting these two processes. However, the exact etiology of AD remains to be fully elucidated. When compared alongside, the immune response in AD closely resembles the central nervous system (CNS) immune changes seen in elderly individuals. It is possible that AD is a pathological consequence of an aged immune system secondary to chronic stimulation by a previous or ongoing insult. Pathological changes like amyloid accumulation and neuronal cell death may reflect this process of immunosenescence as the CNS immune system fails to maintain homeostasis in the CNS. It is likely that future treatments designed to modulate the aged immune system may prove beneficial in altering the disease course. The development of new tests for appropriate biomarkers would also be essential in screening for patients most likely to benefit from such treatments.
Single Immunization of a Vaccine Vectored by a Novel Recombinant Vaccinia Virus Affords Effective Protection Against Respiratory Syncytial Virus Infection in Cotton Rats
Authors: Russell Marsha S. , Thulasi Raman Sathya N. , Gravel Caroline , Zhang Wanyue , Pfeifle Annabelle , Chen Wangxue , Van Domselaar Gary , Safronetz David , Johnston Michael , Sauve Simon , Wang Lisheng , Rosu-Myles Michael , Cao Jingxin , Li Xuguang
Published: September 2021

Respiratory syncytial virus (RSV) is a leading cause of respiratory infections worldwide and disease management measures are hampered by the lack of a safe and effective vaccine against the infection. We constructed a novel recombinant RSV vaccine candidate based on a deletion mutant vaccinia virus platform, in that the host range genes E3L and K3L were deleted (designated as VACVΔE3LΔK3L) and a poxvirus K3L ortholog gene was used as a marker for the rapid and efficient selection of recombinant viruses. The safety of the modified vaccinia virus was investigated by intranasal administration of BALB/c mice with the modified vaccinia vector using a dose known to be lethal in the wild-type Western Reserve. Only a minor loss of body weight by less than 5% and mild pulmonary inflammation were observed, both of which were transient in nature following nasal administration of the high-dose modified vaccinia virus. In addition, the viruses were cleared from the lung in 2 days with no viral invasions of the brain and other vital organs. These results suggest that the virulence of the virus has been essentially abolished. We then investigated the efficiency of the vector for the delivery of vaccines against RSV through comparison with another RSV vaccine delivered by the widely used Modified Vaccinia virus Ankara (MVA) backbone. In the cotton rats, we found a single intramuscular administration of VACVΔE3LΔK3L-vectored vaccine elicited immune responses and protection at a level comparable to the MVA-vectored vaccine against RSV infection. The distinct features of this novel VACV vector, such as an E3L deletion for attenuation and a K3L ortholog for positive selection and high efficiency for vaccine delivery, could provide unique advantages to the application of VACV as a platform for vaccine development.
Consumption of Butylated Starch Alleviates the Chronic Restraint Stress-Induced Neurobehavioral and Gut Barrier Deficits Through Reshaping the Gut Microbiota
Authors: Tian Peijun , Zhu Huiyue , Qian Xin , Chen Ying , Wang Zheng , Zhao Jianxin , Zhang Hao , Wang Gang , Chen Wei
Published: September 2021

The beneficial effect of short-chain fatty acids (SCFAs) on host health has been well recognized based on the booming knowledge from gut microbiome research. The role of SCFA in influencing psychological function is highlighted in recent years but has not been fully elucidated. In this study, the SCFA-acylated starches were used to accomplish a sizeable intestine-targeted release of the SCFAs, and the neurobehavioral, immunological, and microbial effects were further investigated. Acetylated-, butylated-, and isobutylated-starch could attenuate the depression-like behaviors and excessive corticosterone production in chronically stressed mice. Butylated- starch significantly reduced the colonic permeability via increasing the tight junction proteins (including ZO-1, Claudin, and Occludin) gene expression and reduced the level of the inflammatory cytokines (including IL-1β and IL-6). The butylated starch’s neurological and immunological benefits may be derived from the gut microbiome modifications, including normalizing the abundance of certain beneficial microbes (Odoribacter and Oscillibacter) and metabolomic pathways (Tryptophan synthesis and Inositol degradation). The present findings further validate the brain-beneficial effect of butyrate and offer novel guidance for developing novel food or dietary supplements for improving mental health.
Monitoring Plastic Beach Litter by Number or by Weight: The Implications of Fragmentation
Authors: Smith Lauren , Turrell William Richard
Published: September 2021

Eighty surveys of ten Scottish beaches recorded litter sizes and weights. A simple model of fragmentation explains the distribution of plastic beach litter weights, producing a logarithmic cascade in weight-frequencies having a power law exponent of 1.6. Implications of fragmentation are numerous. Heavy litter is rare, light fragments are common. Monitoring by number is sensitive to minimum observable fragment size, age of the litter, and energy of the foreshore. Mean litter item weights should be used to calculate beach plastic loadings. Presence/absence of mega litter can distort monitoring by weight. Multiple surveys are needed to estimate mega litter statistics. Monitoring by weight can change the perception of the importance of litter sources (e.g., in our surveys, contribution from fishing was 6% by number, 41% by weight). In order to introduce consistency between beach surveys using visual methods by number, a standard minimum plastic fragment size should be introduced.
Overexploitation, Recovery, and Warming of the Barents Sea Ecosystem During 1950–2013
Authors: Pedersen Torstein , Mikkelsen Nina , Lindstrøm Ulf , Renaud Paul E. , Nascimento Marcela C. , Blanchet Marie-Anne , Ellingsen Ingrid H. , Jørgensen Lis L. , Blanchet Hugues
Published: September 2021

The Barents Sea (BS) is a high-latitude shelf ecosystem with important fisheries, high and historically variable harvesting pressure, and ongoing high variability in climatic conditions. To quantify carbon flow pathways and assess if changes in harvesting intensity and climate variability have affected the BS ecosystem, we modeled the ecosystem for the period 1950–2013 using a highly trophically resolved mass-balanced food web model (Ecopath with Ecosim). Ecosim models were fitted to time series of biomasses and catches, and were forced by environmental variables and fisheries mortality. The effects on ecosystem dynamics by the drivers fishing mortality, primary production proxies related to open-water area and capelin-larvae mortality proxy, were evaluated. During the period 1970–1990, the ecosystem was in a phase of overexploitation with low top-predators’ biomasses and some trophic cascade effects and increases in prey stocks. Despite heavy exploitation of some groups, the basic ecosystem structure seems to have been preserved. After 1990, when the harvesting pressure was relaxed, most exploited boreal groups recovered with increased biomass, well-captured by the fitted Ecosim model. These biomass increases were likely driven by an increase in primary production resulting from warming and a decrease in ice-coverage. During the warm period that started about 1995, some unexploited Arctic groups decreased whereas krill and jellyfish groups increased. Only the latter trend was successfully predicted by the Ecosim model. The krill flow pathway was identified as especially important as it supplied both medium and high trophic level compartments, and this pathway became even more important after ca. 2000. The modeling results revealed complex interplay between fishery and variability of lower trophic level groups that differs between the boreal and arctic functional groups and has importance for ecosystem management.
Hot Tensile Deformation Behavior of Mg-4Li-1Al-0.5Y Alloy
Authors: Yang Ting , Li Zhuohua , Wang Jinhui , Jin Peipeng
Published: September 2021

The microstructure evolution and deformation mechanism of the as-extruded-annealed Mg-4Li-1Al-0.5Y alloy (denoted as LAY410) were investigated during the hot tensile deformation at the temperatures between 150°C and 300°C with strains from 8 × 10−5 s−1 to 1.6 × 10−3 s−1. The results show that when the strain rate decreases and/or the deformation temperature increases, the peak stress of the alloy gradually decreases, and the elongations to fracture gradually increases. The true stress–strain curves show typical dynamic recrystallization (DRX) softening characteristics. It is observed that the microstructure in the magnesium (Mg) alloy deformed at 150°C is mainly composed of the deformed grains and a few recrystallized grains. The microstructures in the Mg alloy deformed at 200°C consisted of substructures and a slightly increasing number of dynamic recrystallized grains. When the deformation temperature reaches 250°C, the number of recrystallized grains increases significantly, and the microstructures are dominated by recrystallized grains. Moreover, through theoretical calculation and result analysis, the activation energy was about 99.3 kJ/mol, and the hot tensile deformation mechanism was the alternate coordinated deformation mechanism among grain boundary slip (GBS), intragranular slip, and DRX.
Acoustic Wave Reflection Control Based on Broadband Differential Phase Shifters
Authors: Qin Zifu , Liu Xiaohan , Ma Chu
Published: September 2021

Controlling the flow of acoustic waves has broad applications in acoustic imaging, communication, energy harvesting, audio systems, etc. Metasurfaces have been developed for wave control. In this work we propose the design of broadband differential phase shifters for acoustic reflected waves, which can achieve nearly constant phase shift values over a broad frequency ranges. We further demonstrate the design of a broadband differential π phase shifter that works in the frequency range of (10 kHz, 16 KHz) and its applications in acoustic metasurfaces for steering and focusing of reflected acoustic waves. The metasurfaces we designed have the following advantages: 1) The metasurface is formed with binary patterns instead of more complex phase discretization steps, thus the metasurface realization is less complex for fabrication and assembly. 2) The phase shift values of the two basic phase shifter elements are broadband, thus we do not need to fabricate new elements for each frequency. Rearranging the same elements into different location patterns would allow us to tune the working frequency of the metasurfaces.
Patterns of Comorbidity and In-Hospital Mortality in Older Patients With COVID-19 Infection
Authors: Mahmoud Mona , Carmisciano Luca , Tagliafico Luca , Muzyka Mariya , Rosa Gianmarco , Signori Alessio , Bassetti Matteo , Nencioni Alessio , Monacelli Fiammetta ,   The GECOVID Study Group
Published: September 2021

Introduction: Older adults are more susceptible to severe COVID-19, with increased all-cause mortality. This has been attributed to their multimorbidity and disability. However, it remains to be established which clinical features of older adults are associated with severe COVID-19 and mortality. This information would aid in an accurate prognosis and appropriate care planning. Here, we aimed to identify the chronic clinical conditions and the comorbidity clusters associated with in-hospital mortality in a cohort of older COVID-19 patients who were admitted to the IRCCS Policlinico San Martino Hospital, Genoa, Italy, between January and April 2020.

Methods: This was a retrospective cohort study including 219 consecutive patients aged 70 years or older and is part of the GECOVID-19 study group. During the study period, upon hospital admission, demographic information (age, sex) and underlying chronic medical conditions (multimorbidity) were recorded from the medical records at the time of COVID-19 diagnosis before any antiviral or antibiotic treatment was administered. The primary outcome measure was in-hospital mortality.

Results: The vast majority of the patients (90%) were >80 years; the mean patient age was 83 ± 6.2 years, and 57.5% were men. Hypertension and cardiovascular disease, along with dementia, cerebrovascular diseases, and vascular diseases were the most prevalent clinical conditions. Multimorbidity was assessed with the Cumulative Illness Rating Scale. The risk of in-hospital mortality due to COVID-19 was higher for males, for older patients, and for patients with dementia or cerebral-vascular disease. We clustered patients into three groups based on their comorbidity pattern: the Metabolic-renal-cancer cluster, the Neurocognitive cluster and the Unspecified cluster. The Neurocognitive and Metabolic-renal-cancer clusters had a higher mortality compared with the Unspecified cluster, independent of age and sex.

Conclusion: We defined patterns of comorbidity that accurately identified older adults who are at higher risk of death from COVID-19. These associations were independent of chronological age, and we suggest that the identification of comorbidity clusters that have a common pathophysiology may aid in the early assessment of COVID-19 patients with frailty to promote timely interventions that, in turn, may result in a significantly improved prognosis.
Antibacterial Activity of Pediocin and Pediocin-Producing Bacteria Against Listeria monocytogenes in Meat Products
Authors: Khorshidian Nasim , Khanniri Elham , Mohammadi Mehrdad , Mortazavian Amir M. , Yousefi Mojtaba
Published: September 2021

One of the most important challenges in the food industry is to produce healthy and safe food products, and this could be achieved through various processes as well as the use of different additives, especially chemical preservatives. However, consumer awareness and concern about chemical preservatives have led researchers to focus on the use of natural antimicrobial compounds such as bacteriocins. Pediocins, which belong to subclass IIa of bacteriocin characterized as small unmodified peptides with a low molecular weight (2.7–17 kDa), are produced by some of the Pediococcus bacteria. Pediocin and pediocin-like bacteriocins exert a broad spectrum of antimicrobial activity against Gram-positive bacteria, especially against pathogenic bacteria, such as Listeria monocytogenes through formation of pores in the cytoplasmic membrane and cell membrane dysfunction. Pediocins are sensitive to most protease enzymes such as papain, pepsin, and trypsin; however, they keep their antimicrobial activity during heat treatment, at low temperatures even at −80°C, and after treatment with lipase, lysozyme, phospholipase C, DNase, or RNase. Due to the anti-listeria activity of pediocin on the one hand and the potential health hazards associated with consumption of meat products on the other hand, this review aimed to investigate the possible application of pediocin in preservation of meat and meat products against L. monocytogenes.
Prokaryotic and Eukaryotic Fecal Microbiota in Irritable Bowel Syndrome Patients and Healthy Individuals Colonized With Blastocystis
Authors: Nourrisson Céline , Scanzi Julien , Brunet Julie , Delbac Frédéric , Dapoigny Michel , Poirier Philippe
Published: September 2021

Blastocystis is the most frequently isolated protozoan from human stool. Its role in human health is still debated, and a high prevalence was reported in irritable bowel syndrome (IBS) subjects, suggesting a potential link with microbiota. In the present study, we aimed to investigate prokaryotic and eukaryotic microbiota in both IBS-C (constipated) and healthy individuals. We recruited 35 IBS-C patients and 23 healthy subjects, from which 12 and 11 carried Blastocystis, respectively. We performed 16S and 18S rRNA high-throughput sequencing on feces. Whereas we did not observe differences between infected and non-infected controls, several phyla were significantly modified in IBS-C patients according to the presence of Blastocystis. Tenericutes phylum and Ruminococcaceae family were especially increased in Blastocystis carriers. Furthermore, colonization with Blastocystis was associated with discrete changes in the microbial eukaryome, particularly among the Fungi taxa. Depending on the group of patients considered, the mycobiota changes do not go in the same direction and seem more deleterious in the IBS-C group. These results encourage further in vivo and in vitro investigations concerning the role of Blastocystis in the gut environment.
Combined Untargeted and Targeted Metabolomics Approaches Reveal Urinary Changes of Amino Acids and Energy Metabolism in Canine Babesiosis With Different Levels of Kidney Function
Authors: Kuleš Josipa , Rubić Ivana , Beer Ljubić Blanka , Bilić Petra , Barić Rafaj Renata , Brkljačić Mirna , Burchmore Richard , Eckersall David , Mrljak Vladimir
Published: September 2021

Canine babesiosis is a tick-borne disease with a worldwide distribution, caused by the haemoprotozoan parasites of the genus Babesia. One of the most prevalent complication is acute kidney injury, and an early diagnosis of altered kidney function remains a challenge for veterinary practice. The aim of this study was to assess the urine metabolic profile from dogs with babesiosis and different degree of kidney function using untargeted and targeted MS-based metabolomics approaches. In this study, 22 dogs naturally infected with Babesia canis and 12 healthy dogs were included. Untargeted metabolomics approach identified 601 features with a differential abundance between the healthy group and groups of dogs with babesiosis and different level of kidney function, with 27 of them identified as a match to known standards; while targeted approach identified 17 metabolites with significantly different concentrations between the groups. A pattern of significantly altered metabolites referring to the inflammatory host response, oxidative stress, and energy metabolism modulation in babesiosis was presented. Our findings have demonstrated that kidney dysfunction accompanying canine babesiosis was associated with changes in amino acid metabolism, energy metabolism, fatty acid metabolism, and biochemical pathways such as urea cycle and ammonia detoxication. These findings will enable the inclusion of urinary markers for the detection and monitoring of renal damage in babesiosis, as well as in other similar diseases.