bluuebunny/crossref_metadata_2025 huggingface dataset:
Evolving appropriate zinc fertilization strategy for rice-rice (Oryza sativa) cropping system in Cauvery Delta Zone
Authors: Rahale C Sharmila
Published: September 2019

Six field experiments were conducted at Tamil Nadu Rice Research Institute, Aduthurai to evolve suitable zinc fertilization method for rice - rice (Oryza sativa L.) cropping system in Cauvery delta zone. The treatment includes: T1 : Control, T2 : 100 g zinc sulphate (ZnSO4)/cent in nursery alone., T3 : root dipping alone in 2 % zinc oxide (ZnO) solution, T4 : 25 kg ZnSO4 ha-1, T5 : 37.5 kg ZnSO4 ha-1, T6 : 25 kg ZnSO4 ha-1+ Farm Yard Manure (FYM) 12.5 t ha-1, T7 : 25 kg ZnSO4 ha-1 + Green Leaf Manure (GLM) 6.5 t ha-1, T8 : Tamil Nadu Agricultural University Micro Nutrient (TNAU MN) mixture 25 kg ha-1 as Enriched Farm Yard Manure (EFYM), T9 : TNAU MN mixture 37.5 kg ha-1 as EFYM, T10 : Foliar spray of 0.5% ZnSO4 + 1 % urea at tillering and panicle initiation stage, T11 : 100g ZnSO4 /cent in nursery alone + Foliar spray of 0.5 % ZnSO4 + 1 % urea at tillering and panicle initiation stage (T2+ T10), T12 : root dipping alone in 2 % ZnO solution + Foliar spray of 0.5 % ZnSO4 + 1 % urea at tillering and panicle initiation stage (T3 + T10), T13: 100 g ZnSO4 /cent in nursery alone + root dipping alone in 2 % ZnO solution + Foliar spray of 0.5 % ZnSO4 + 1 % urea at tillering and panicle initiation stage (T2 + T3 + T10). The treatments T5, T6, T7 and T9 were skipped in rabi season to know the residual effect of these treatments in the subsequent season. Among the treatment combinations, application of 25 kg ZnSO4 ha-1 + FYM 12.5 t ha-1 recorded higher grain yield in both kharif (6232 kg ha-1) and rabi (6236 kg ha-1) seasons. The same treatment combination recorded higher Zn content and Zn uptake as well. Regarding soil nutrient content, the same treatment recorded higher N, P and K content. This treatment was followed by application of 25 kg ZnSO4 ha-1 + green leaf manure 6.5 t ha-1. The experimental findings suggested that combination of organic and inorganic sources not only increased the yield but also improves soil health in Cauvery delta zone.
Uptake of cadmium by rice crop in sewage irrigated soil
Authors: Kumar Vipin , Prasad RK , Suman SN , Dash BP
Published: September 2019

The results of green house experiment indicated that the yield of root, shoot and total dry matter of rice increased from 0.18 to 0.23 g pot-1, from 1.49 to 1.87 g pot-1 and from 1.67 to 2.09 g pot-1, respectively with increasing levels of Cd-FA (fulvic acid). The dry matter yields, concentrations and uptake of Cd by rice were significantly and positively correlated with organic carbon and DTPA-extractable Cd in soil. Root density and root length of rice exhibited positive and significant correlation with organic carbon, CEC, EC and negative correlation with clay content of the soil. On the other hand, root radius and half distance between roots showed significant and negative correlation with these parameters. DTPA-extractable Cd increased with increasing levels of Cd-FA application in soils. A regression model developed was found to predict the uptake of Cd on the basis of root parameters (root radius, root length, root density, half distance between roots) and diffusion coefficient of Cd in soils with almost 95 % level of confidence.
Pengaruh Promosi dan Harga Terhadap Keputusan Pada Pembelian PT. Shopee International Indonesia Di Kota Manado
Authors: Tulangow Sharen G. , Tumbel Tinneke M. , Walangitan Olivia F. C.
Published: September 2019

This study aims to determine the effect of promotion and price on purchasing decisions at PT. Shopee International Indonesia in the city of Manado, where Shopee consumers in the city of Manado also often shop through this application by looking at the promotions that PT. Shopee International Indonesia often holds and the prices offered so that in purchasing decisions there are several factors that influence in it, and this research the writer takes the promotion and price factors. In this research quantitative associative research is used with data collection techniques by observation and questionnaire. The analysis technique used is the validity test, reliability test, normality test, multiple linear regression analysis, coefficient of determination analysis. In testing the hypothesis using partial test and simultaneous test. Based on the results obtained from this study, it shows that the influence of promotion is 52.9% and the influence of price is 40.3%. and the effect of promotion and price simultaneously that is equal to 56.6% while the remaining 43.4% is explained by other variables not included in this study that include products, places, people, processes, advertising, physical evidence.
Anciano con Diabetes y factores asociados
Authors: Morros-González Elly , Borda Miguel Germán , Reyes-Ortiz Carlos , Chavarro-Carvajal Diego , Cano-Gutierrez Carlos
Published: September 2019

Objetivo: Conocer el Auto-reporte de Diabetes en la población de adultos mayores de la ciudad de Bogotá y su asociación con variables sociodemográficas, del estado de salud y medidas antropométricas.

Diseño: Estudio observacional y poblacional de corte transversal

Marco de referencia: Datos del estudio SABE (Encuesta de Salud, Bienestar y Envejecimiento) Bogotá, Colombia.

Participantes: 1999 personas de 60 años o más de Bogotá, Colombia.

Mediciones: Variables sociodemográficas, enfermedades, hábitos, actividad física, tratamiento, evaluación cognitiva y afectiva, estado funcional y medidas antropométricas. Se realizaron análisis bi-variados y multivariados con el paquete estadístico SAS (versión 9.4).

Resultados: Las personas tenían edad promedio de 71.2 ±8.0 y 62% eran mujeres; el 17.5% auto-reportó diagnóstico de Diabetes. En análisis multivariados, Diabetes estuvo asociada con la Hipertensión (OR = 1,62 95% intervalos de confianza IC = 1,20-1,18), Catarata (OR = 1,71 95% IC = 1,24-2,36) y con el aumento de perímetro abdominal (OR = 1,03 95% IC = 1,02-1,04). También estuvo asociada con una menor fuerza de agarre (OR = 0,98 95% IC = 0,96-0,99) y en menor medida con aquellos mayores de 85 años (OR 0.34, 95% IC = 0.16-0.72)

Conclusión: La prevalencia de DM en Bogotá se encuentra presente en casi un quinto de la población encuestada y representa una de las prevalencias más altas dentro de las ciudades Latinoamericanas y del Caribe en donde se llevó a cabo el Estudio SABE.
Signo del calcio
Authors: Oñate Rosado Hermes Luis , Correa Anderson , Costa Filho Francisco Flavio
Published: September 2019

Paciente masculino de 85 años de edad, nacionalidad Japonesa, ingresa al servicio de urgencias del Hospital Dante Pazzanese de Cardiologia con cuadro clínico de 12 horas de evolución consistente en dolor torácico opresivo, inicio gradual, intensidad moderada, duración mayor de 30 minutos, no irradiado, no mejora con el reposo o con el uso de nitratos asociado a disnea de medianos esfuerzos, niega síntomas neurovegetativos. Antecedentes de importancia Hipertensión arterial, Enfermedad renal crónica estadio 4 (TFG 22 ml/min/1.73m2 por CKD EPI), Insuficiencia cardiaca con fracción de eyección reducida (FE 22%). Al examen físico paciente normotenso, bradicárdico, ausculta con soplo sistólico en foco Mitral grado 3/6, sin frémito; sin signos de bajo gasto cardiaco. Realizada impresión diagnostica de Síndrome coronario agudo por lo cual fue solicitado EKG de 12 derivaciones, marcadores de necrosis miocárdicas y Radiografía de tórax. El EKG revela bradicardia sinusal, hemibloqueo anterior izquierdo, sin signos de lesión o isquemia aguda, marcadores negativos y radiografía de tórax muestra ensanchamiento mediastinal y signo del calcio positivo por lo que sospechamos en Síndrome aórtico agudo y es solicitado Eco transesofágico mostrando dilatación discreta de la aorta ascendente 40 mm, arco aórtico de difícil visualización, dilatación moderada de la aorta descendente 44 mm, imagen sugestiva de hematoma intramural aórtico iniciando después del nacimiento de la arteria subclavia izquierda y extendiéndose hasta el tercio proximal de la aorta torácica descendente, midiendo 11 mm de espesor. El paciente posteriormente es sometido a Angiotomografia de tórax con medidas de prevención de nefrotoxicidad inducida por contraste y es evidenciado presencia de hematoma en la pared de la aórta torácica descendente que inicia después de la emergencia de la arteria subclavia izquierda hasta la emergencia de las arterias renales. Fue solicitada valoración por cirugía vascular y optado por tratamiento clínico dada la fase etaria del paciente y la presencia de comorbilidades. Actualmente continúa en seguimiento con Cardiologia, Nefrologia y Cirugía vascular.
Evaluación del desempeño pronóstico de dos puntajes de predicción de mortalidad a siete días en pacientes adultos oncológicos críticamente enfermos admitidos en una unidad de cuidados intensivos
Authors: Rojas Ruiz Ingrid Tatiana , Méndez Toro Arnold , Rincón Bonilla Jose Fernando
Published: September 2019

Introducción

Los sistemas de puntuación para predicción se han desarrollado para medir la severidad de la enfermedad y el pronóstico de los pacientes en la unidad de cuidados intensivos. Son útiles para la toma de decisiones clínicas, la estandarización de la investigación y la comparación de la calidad de la atención.

Materiales y métodos

Estudio observacional analítico de cohorte en el que revisaron las historias clínicas de 283 pacientes oncológicos admitidos a la unidad de cuidados intensivos (UCI) durante enero de 2014 a enero de 2016, a quienes se les estimo la probabilidad de mortalidad con los puntajes pronósticos APACHE IV y MPM II, se realizó regresión logística binaria con las variables de los modelos es sus estudios originales, se determinó calibración, discriminación y se calcularon criterios de información Akaike AIC y Bayesiano BIC.

Resultados

En la evaluación de desempeño de los puntajes pronósticos APACHE IV mostro mayor capacidad de predicción (AUC = 0,95) versus MPM II (AUC = 0,78), los dos modelos mostraron adecuada calibración con estadístico de Hosmer y Lemeshow para APACHE IV (p = 0,39) y para MPM II (p = 0,99). El delta BIC es 2,9 mostrando evidencia positiva en contra de APACHE IV. El estadístico AIC es menor para APACHE IV indicando que es el puntaje con mejor ajuste a los datos.

Conclusiones

APACHE IV tiene un buen desempeño en la predicción de mortalidad de pacientes oncológicos críticamente enfermos. Es una herramienta útil para el clínico en su labor diaria, al permitirle distinguir los pacientes con alta probabilidad de mortalidad.
CORYNEBACTERIUM: FEATURES OF THE STRUCTURE OF THE BACTERIAL CELL
Authors: Kharseeva G. G. , Voronina N. A.
Published: September 2019

In a review of the features of the bacterial cells are Corynebacterium structure: characterized by an upper layer, highly organized cell wall, cytoplasmic membrane, cytoplasm, nucleoid. Described in detail the structure of the upper layer containing pili (fimbriae), microcapsule surface proteins - PS-2, DIP1281, 67-72r protein (hemagglutinin), porins, sialidase (neuraminidase). These components are the ability to initiate a serial of Corynebacterium work with the host cell, followed by colonization. It submitted a detailed description of the structure and functions of cell wall structures - cord factor, which is a second barrier permeability; arabinogalactan, peptidoglycan, lipomannan and lipoarabinomannan. The structure and function of the cytoplasmic membrane as the main diffusion barrier cell cytoplasm and the genome of Corynebacterium. Presented different molecular genetic methods for the identification and differentiation of closely related species of Corynebacterium.
CONSTRUCTION OF A TEST-SYSTEM WITH NANOPARTICLES OF COLLOID SILVER FOR DETECTION OF PSEUDOTUBERCULOSIS AND INTESTINAL YERSINIOSIS FOR CAUSATIVE AGENTS IN DOT-IMMUNOASSAY
Authors: Zagoskina T. Yu. , Chesnokova M. V. , Klimov V. T. , Popova Yu. O. , Markov E. Yu. , Starikova O. A.
Published: September 2019

Aim. Construction of an immunologic test-system for detection of causative agents of entero-pathogenic yersinia {Yersinia pseudotuberculosis and Y. enterocolitica) by dot-immunoassay. Materials and methods. Nanoparticles of colloid silver sized 5 - 9 nm were used as a marker of specific antibodies. IgG fraction was isolated from commercial antisera to Y. pseudotuberculosis (0:1) and Y. enterocolitica (0:3 and 0:9). Testing of the obtained test-system was carried out on 20 strains of Y. pseudotuberculosis and Y. enterocolitica (10 of each species). Results. Dot-analysis had a specific character and detected enteropathogenic yersinia at a level of 5Т05 - 8T06 CFU/ml (100 - 1000 CFU in sample). Wherein cross-reaction with heterologic studied microorganisms - Escherichia coli, Salmonella typhimurium, Shigella flexneri, Yersiniapestis EV - was not observed. A possibility of simultaneous detection and serotyping of Y. enterocolitica is shown, that is necessary for confirmation of their epidemic significance. Conclusion. The developed test-systems allow to study micro volumes of the samples under study (1 pi), are express (1.5 - 2 h), highly sensitive and specific, technically simple and do not require the use of high-cost equipment, special training of the staff, may be successfully used in practical healthcare in laboratories with varying equipment levels.
ANTIBIOTIC SUSCEPTIBILITY AND MOLECULAR PROPERTIES OF STRAIN STREPTOCOCCUS PYOGENES FROM PATIENTS WITH SOFT TISSUE INFECTIONS AND ANGINA
Authors: Briko S. I. , Glushkova E. V. , Kleymenov D. A. , Dmitrieva N. F. , Lipatov K. V. , Devyatkin A. V. , Malikov V. E.
Published: September 2019

Aim. To compare the molecular properties and antibiotic susceptibility of GAS isolates in patients with respiratory and soft tissue infections. Materials and methods. 86 GAS isolates from patients with respiratory infections and 91 isolates with soft tissue infections were studied. The antimicrobial susceptibility profile of six antibiotics (clindamycin, erythromycin, azithromycin, clarithromycin, tetracycline, levofloxacin) was performed by the microdilution method. PCR and sequencing were used in emm-typing and detection SpeA, SpeB and SpeC genes. Results. Emm-types: 49, 66, 88 and 169 were the most prevalent in patients with soft tissue infections, and 1, 3, 12, 28, 75, 89 - in patients with respiratory infections. One strain was new. Isolates were representatives of the three patterns (A-C, D, E). 116 strains of both groups comprised to pattern E. 15 strains (21%) exclusively from soft tissue infections comprised to pattern D. More than half of isolates from patients with soft tissue infections had resistant to tetracycline. Resistance to macrolides was determined in both groups. In each of the studied groups were isolated strains with multidrug resistant. Eryphrogenic toxins gene A and C was more frequently in respiratory isolates. Conclusion. The group of respiratory GAS was less heterogenic in emm-types composition. Pattern D was not contain any pharyngeal GAS strains. Eryphrogenic toxin gene speA was identified twice as likely in respiratory isolates. The use of tetracycline and macrolides would be ineffective in approximately half of the cases among the patients with soft tissue infections.
POSSIBILITIES OF USE OF IMMUNE MODULATORS IN COMPLEX THERAPY OF COMMUNITY-ACQUIRED PNEUMONIAS
Authors: Korovkina E. S. , Kostinov M. P. , Kazharova S. V.
Published: September 2019

Community-acquired pneumonia (CAP) has been one the most pressing issues of healthcare due to high morbidity and mortality that are stable. Analysis of literature shows similar trends in the character of immune response of patients with CAP, precisely - formation of immune response via humoral route (Th2) with suppression of cell immune response (Thl), whereas lack of cellular and humoral mechanisms of immunity and dysbalanced cytokine portion determine severity of the infection course. Inclusion of immune modulators into traditional therapy allows to enhance the response to the therapy already being administered. One of the national immune modulators is azoximer bromide (polyoxidonium), its administration allows to reduce the length and severity of the disease, as well as reduce the frequency of development of prolonged forms. Also, immune modulators of bacterial origin have received special interest as of late, and antibacterial therapeutic vaccines are being constructed based on them. Immunovac VP-4 polycomponent vaccine is distinguishable among the national preparations. Clinical-immunological effect of the vaccine has been studied in recent years on different pathologies and methods of administration in adults and children. Features of Immunovac VP-4 effect confirm the primary character of alterations in the immune system, that is the background of a certain pathology and is the reason for inclusion of immune modulating preparations into basic therapy of the diseases.
YOUNG AGE CHILDREN WITH INTESTINE DYSBIOSIS AS CARRIERS OF ENTEROAGGREGATIVE ESCHERICHIA COLI
Authors: Makarova M. A. , Suzhaeva L. V. , Kaftyreva L. A.
Published: September 2019

Aim. Study the prevalence of diarrhea-genic E. coli of the enteroaggregative group in children with intestine dysbiosis. Materials and methods. PCR method was used to study virulence factors in 511 strains of E. coli isolated during bacteriologic study of feces samples from 393 children aged less than 2 years. Sensitivity to antibiotics was determined by disc-diffusion method, results interpretation - according to clinical recommendations Determination of sensitivity of microorganisms to antimicrobial preparations, 2015. Results. 23 enteroaggregative E. coli strains were identified (EAggEC). All the strains had aaf gene coding aggregative-adhesion fimbriae and 4 other genes (aggR, ast, aap, aatA) in various combinations coding virulence factors EAggEC. 19 strains (87%) were not sensitive to antimicrobial preparations. Resistance to extended spectrum cephalosporins was determined by the production of extended spectrum beta-lactamase (ESBL) of CTX-M genetic family and AmpC cephalosporinase. Conclusion. Results of the study have shown that 6% of children with intestine dysbiosis are EAggEC carriers, that gives evidence on the necessity of detection of EAggEC strains - a novel group of diarrhea-genic E. coli not only in patients with diarrhea syndrome, but also using intestine dysbiosis.
DETECTION OF MARKERS OF HERPES VIRUS INFECTION AND PNEUMOCYSTOSIS IN CHILDREN FROM HIV-INFECTED MOTHERS
Authors: Savinkov P. A. , Rybalkina T. N. , Karazhas N. V. , Boshyan R. E. , Kalugina M. Yu. , Kornienko M. N. , Rusakova E. V. , Burmistrov E. M. , Soldatova I. A.
Published: September 2019

Aim. Study the role of herpes viruses and pneumocystis in infectious complications in children from HIV-infected mothers. Materials and methods. Sera and blood cells from 59 children from HIV-infected mothers were studied for the presence of various markers of herpes virus infections and pneumocystosis by a complex of methods of modem laboratory diagnostics. Results. Frequency of detection of markers of herpes virus infection was from 10% for chicken pox in children with non-final HIV test to 93% for herpes simplex virus in HIV-infected children from closed organized groups. Signs of active infection in children with laboratory confirmed HIV infection were diagnosed 2.5 times more frequently for HSV infection and chicken pox and 1.8 times more frequently for HHV-6 and pneumocystis than in children with non-final HIV test. Markers of various disease stages with opportunistic infections (01) were detected in children with confirmed HIV-infection: primary acute and latent forms of the infection, reactivation, reconvalescence, whereas in children with non-final HIV test maternal antibodies against herpes virus and pneumocystis predominated. Markers of active infections excluding HSV and HHV-6 were more frequently detected in children from families than in children from closed organized groups. Conclusion. The feature detected - a lower percentage of detection of markers of active forms of 01 in HI V-infected children from social institutions - is determined by the fact that observation of these children is carried out by medical personnel that have the knowledge and experience of prophylaxis of infectious complications in HIV-infected children, whereas quality anti-epidemic regimen is frequently not maintained regarding home children with HIV infection. Another factor facilitating spread of opportunistic infections is the asocial lifestyle of most of the examined families. These data dictate the necessity of enhancement of anti-epidemic regimen and prophylaxis of opportunistic infections in family loci. Not only HIV-infected children, but also all the family members should be examined for markers of herpes virus infection and pneumocystosis in order to detect sources of the infection and timely execution of the prophylaxis measures.
PROBLEMS OF FINAL PHASE POLIOMYELITIS ERADICATION PROGRAMME
Authors: Chernyavskaya O. P. , Briko N. I.
Published: September 2019

Never before has the world community were not so close to the goal - poliomyelitis eradication. In 2016, the world recorded only 37 cases in the three endemic countries: Pakistan, Afghanistan and Nigeria. Despite the great progress in the final stages of poliomyelitis eradication have problems: the emergence and circulation of vaccine-derived poliovirus and related diseases, vaccine-associated paralytic poliomyelitis, social and political factors affecting of vaccination.
MOLECULAR AND GENETIC FEATURES OF THE METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS (MRSA) - CAUSATIVE AGENTS OF PURULENT DISEASES AT CANCER PATIENTS
Authors: Khokhlova O. E. , Peryanova O. V. , Bobrova O. P. , Sergeeva V. K. , Modestov A. A. , Eremeeva O. G. , Potkina N. K. , Kapshuk D. N. , Alabusheva A. V. , Yamamoto T. ..
Published: September 2019

Aim. Study of the molecular and genetic features of the MRSA, causative agents of purulent diseases at cancer patients. Materials and methods. In the work were used - bacteriological method, molecular genetic methods (PCR, M-PCR, sequencing). Results. MRSA share among staphylococcal infections at cancer patients in 2003 - 2007 has made 37,6%, and in 2010 - 2015 has increased up to 51,3%. At a research of molecular and genetic features of the allocated MRS A ] strains, have established, existence of two main clones: ST239/spa3(t037)/SCCmecIIIA and ST8/ . spal(t008)/SCCmecIVc. Representatives of clones differed in resistance level to antibiotics. Conclusion. In cancer patients in Krasnoyarsk, the distribution of two variants of MRSA-ST239Kra$ and ST8Kras, corresponding to the variants common in the Krasnoyarsk region, was revealed. i
FLOW-CYTOFLUORIMETRIC ANALYSIS OF CYTOTOXICITY OF YERSINIA PESTIS STRAINS
Authors: Sazanova E. V. , Shmelkova T. P. , Kravtsov A. L. , Malyukova T. A. , Popov Yu. A.
Published: September 2019

Aim. Study cytotoxic effect of Yersinia pestis strains with various degree of virulence on leukocytes of whole defibrinated human blood. Materials and methods. 15 strains of Y. pestis were used in the study. DNA histograms were registered using flow cytofluorimeter. Results. Possibility of using cytotoxicity to differentiate Y. pestis into virulent and avirulent wfas experimentally proven. The data obtained was comparable with LD5o for mice and did not contradict molecular-genetic characteristics of the strains. Conclusion. The approach proposed allows to obtain reliable results and is perspective for evaluation of virulence for Y. pestis strains.
EFFECT OF METHODS OF IMMOBILIZATION OF ERYTHROPOIETIN ON THE SENSITIVITY FOR THE DETECTION OF SPECIFIC IgG TO EPO IN EXPERIMENTAL ANIMALS SERA
Authors: Kudryashova A. M. , Borisova O. V. , Mikhailova N. A. , Lonshakov D. V. , Katlinsky A. V.
Published: September 2019

Aim. The study of the effect of the erythropoietin coating procedure on sensitivity using the same secondary detection methods to quantify anti-EPO IgG positive animal sera. Materials and methods. Sera from experimental animals - rabbits and guinea pigs - after rhEPO injection were used. The methods includes directly coated ELISA and two types of immunochemical immobilization: capturing biotinylated rhEPO on streptavidin coated microtiier plates and capturing rhEPO via a specific antibody Results. Immunochemical rhEPO immobilization results in a sensitivity from 2 to 10 of magnitude higher than direct coating of rhEPO. Conclusion. Our findings show that the method of rhEPO immobilization to microtiter plates is a critical determinant for the sensitivity of ELISA used for measuring anti-EPO antibodies. Assays in which rhEPO was captured via a specific mAb, or in which biotinylated rhEPO was captured via streptavidin, are preferred to detect serum antibodies to native structural state.
BIOLOGICAL SAFETY OF PUBLIC EVENTS: FEATURES OF LABORATORY DIAGNOSTICS
Authors: Efremenko D. V.
Published: September 2019

To scientifically prove the nomenclature of the priority pathogenic biological agents (PBA) and the infectious diseases caused by them for laboratory diagnostics when ensuring biological safety of public events. PBA assessment (viruses, bacteria, biological toxins I - IV of groups of pathogenicity) was carried out, using the criteria allowing to predict their potential negative impact at the qualitative level. The evidence-based nomenclature of PBA posing the greatest threat of biological safety regardless oftheir confmedness to the territory of risk and time of risk is developed. The list included the bacterial (plague, anthrax, cholera) and viral agents (orthopoxviruses, filoviruses, arenaviruses, coronaviruses, orthomyxoviruses) of the I - II groups of the pathogenicity. The nomenclature of the widespread natural and focal (tularemia, leptospirosis, the Q fever, hemorrhagic fever with a renal syndrome, ornithosis) and the ubiquitous (brucellosis, hemorrhagic colibacillosis, a hemolytic-uremic syndrome, intestinal yersiniosis, pseudotuberculosis, Legionnaires' disease) infections capable to cause the serious complication of the sanitary and epidemiologic situation is prepared. On the basis of the carried-out assessment of dangerous biological factors the universal list of PBA for priority ensuring readiness for laboratory diagnostics during public events was created.
BACTERICIDAL EFFECT OF HUMAN SERUM ON BORRELIA MIYAMOTOI, CAUSATIVE AGENT OF IXODES TICK-BORNE BORRELIOSIS (ITBB-BM)
Authors: Platonov A. E. , Koetsveld J. .. , Stukolova O. A. , Dolgova A. S. , Kolyasnikova N. M. , Toporkova M. G. , Sarksyan D. S.
Published: September 2019

Aim. Our aim was to study the bactericidal effect of human serum on Borrelia miyamotoi in vitro. Materials and methods. B. miyamotoi spirochetes (strains HT31 and LB-2001) were incubated in non-immune serum of healthy donors (SHD) and in heat inactivated complement-depleted SHD, as well as in serum samples of the patients recovered from ITBB-BM. The viability, that is motility, of borrelia after incubation was investigated by dark-field microscopy. The levels ofserum antibody to B.miyamofoi-specificproteins (GlpQ enzyme and four variable major proteins Vlpl5/16, Vlpl8, Vspl, and Vlp5) were measured by specially designed plane protein microarray. Results. Borrelia fully retain their viability in non-immune SHD, but their motility is partially or completely suppressed by the addition of serum from ITBB-BM convalescents or rabbit antibodies to Д. miyamotoi. The immobilizing effect of the immune serum is substantially inhibited by its heat-inactivation, which indicates that immobilizing effect is mediated by the complement system. Conclusion. Antibody-dependent complement-mediated bactericidal action ofhuman blood serum is probably not the only and 100% effective mechanism for human defense against B. miyamotoi infection, but requires support from cellular immunity.
ANALYSIS OF LOWER RESPIRATORY TRACT INFECTIONS WITH A RESEARCH OF A MICROBIAL LANDSCAPE OF MATERIAL AT HIV-POSITIVE PATIENTS
Authors: Puzyreva L. V. , Rodkina L. A. , Mordyk A. V. , Konchenko V. D. , Dalabaeva L. M.
Published: September 2019

Aim. Study of frequency, the characteristic of lower respiratory tract infections, and results of microbiological researches of biomaterial at HIV-positive patients. Materials and methods. Materials of the Infectious hospital No. 1 of Omsk in 5 years (2012 - 2016) were used. Results. From the treated 1926 HIV-positive patients, lower respiratory tract infections occurred in 538 patients. 45,2% at which lethality was 18,1% fell to the share of bacteriemic pneumonia. The analysis of results of microbiological researches of biomaterials at HIV-positive patients was carried out. In a sputum Staphylococcus aureus, Klebsiella pneumoniae, Enterococcus faecalis and other microorganisms was most often allocated. Streptococcus viridans and mushrooms of the sort Candida were a frequent find in biomaterial. Conclusion. The received results demand further researches for comparison of clinicoradiological implications and result of a microbiological research of biomaterials of HIV-positive patients.
THE MECHANISMS OF BACTERICIDAL ACTION IMPACT IN COMMON ANTIBACTERIAL EFFECTS OF METAL CATIONS IN CULTURE OF STREPTOCOCCUS PYOGENES
Authors: Cheknev S. B. , Vostrova E. I. , Kisil S. V. , Sarycheva M. A. , Vostrov A. V.
Published: September 2019

Aim. The work was performed with the purpose to study an inhibitory action of millimolar concentrations of divalent metal ions, which differ by primary mechanisms of their toxicity, on the culture of S.pyogenes. Materials and methods. Suspensions of S.pyogenes bacteria which contained 108 CFU/ml were sown by the lawns into the standard Petri dishes coated with the supplemented Nutrient Agar. 30 min later the salt solutions of divalent metals were added by the 5 pl drops on the surfaces of the lawns with use of 36-channel stamp replicator. The salt solutions contained the metals tested at the concentrations ranged between 5x10-3 M to 5x10-1 M. Then the dishes with bacterial cultures were incubated for 24 hrs at 37°C followed by measuring diameter of the area of culture growth inhibition. The probes of material obtained from the centers of the stunting areas were passed into the centrifuge tubes with the supplemented Nutrient Broth, incubated for up to five days at 37°C and tested for the Broth clarity. Results. In presence of the metal concentrations ranged between 50 to 500 mM inhibitory action towards S.pyogenes bacteria was registered as relatively low due to the effects of copper or ferrous ions, as intermediate due to the presence of cobalt, nickel or manganese, and as high due to the effects of zinc ions. At the same time ferrous or copper ions demonstrated high bactericidal activity, zinc ions showed relatively low one, whereas manganese, nickel or cobalt were characterized by the lack of bactericidal action registered. Conclusion. Inhibitory action of heavy metal divalent ions on the lawns of S.pyogenes cultures probably includes bacteriostatic and bactericidal components which impact is determined by primary mechanisms of the ions toxicity.
THE EFFECT OF N-ACETYL-L-CYSTEINE ON BIOFILM OF VIBRIO CHOLERAE
Authors: Duvanova O. V. , Mishankin B. N. , Titova S. V. , Korneeva L. A.
Published: September 2019

Aim. To study the effect of N-acetyl-L-cysteine on biofilm of V. cholerae of different sero-groups isolated from various sources and with different epidemiological significance (the presence/ absence of the ctx AB genes and tcpA). Materials and methods. Bacterial eiiltiire of Vibrio cholerae El Tor O1 and O139 serogroups were grown as biolms. We have estimated the influence of the drug N-acetyl-L-cysteine at a concentration of 0.5 - 4 mg/ml on the formation, of the formed biofilm and in planktonic form. Results. Discovered antibacterial activity of N-acetyl-L-cysteine. Noted that it was influenced as in the formation, of the already formed biofilm and the planktonic form, the representatives of the Vibrio cholerae El Tor O1 and O139 serogroups in concentrations of 2 - 4 mg/ml, showing an antibacterial effect regardless of the presence/absence of genes ctx and tcpA AB. Conclusion. Identified the antibacterial action of the drug N-acetyl-L-cysteine against biofilm of V. cholerae indicates the desirability of considering the possibility of using drug therapy in cases variety of diseases caused by causative agents II - IV groups pathogenicity.
EPIDEMIOLOGICAL FEATURES OF IXODES TICK-BORNE BORELIOSES IN THE KRASNOYARSK TERRITORY IN THE CONTEXT OF SEARCHING FOR THE CASES OF INFECTION CAUSED BY BORRELIA MIYAMOTOI
Authors: Titkov A. V. , Platonov A. E. , Stukolova O. A. , Mironov K. O. , Dmitrieva G. M. , Kostrykina T. V. , Sorokina O. V. , Chernykh V. I. , Minoranskaya N. S.
Published: September 2019

Aim. Estimation of the prevalence of Ixodes tick-borne borreliosis (ITBB) without erythema migrans in the Krasnoyarsk Territory in comparison with the prevalence of ITBB with erythema, that is Lyme disease (LD). Detection of cases of «new» infection caused by Borrelia miyamotoi (ITBB-BM) among patients with suspected tick-borne infection, primarily among patients with ITBB without erythema (ITBB-W/O-E). Materials and methods. We analyzed the database of Rospotrebnadzor containing individualized information about cases of ITBB officially registered in the Krasnoyarsk Territory in 2011 — 2016. Blood samples from 18 patients with suspected of tick-borne infection hospitalized in the Krasnoyarsk Emergency Hospital in 2017 were collected and studied. ITBB-BM was diagnosed if B. miyamotoi DNA was found in patient’s blood and/or the antibodies to specific antigens of B. miyamotoi were detected. Results. With an average annual incidence rate of ITBB of about 10 cases per 100,000 population, the proportion of ITBB-W/O-E among all ITBB in the Krasnoyarsk Territory is about 45%. Epidemiological characteristics of ITBB-W/O-E and LD are similar, but these diseases are very different clinically. All cases of ITBBW/O-E and some cases of tick-borne infections of unknown etiology, subjected to our enhanced laboratory diagnostics, were caused by B. miyamotoi. Conclusion. ITBB-W/O-E is a widespread disease in the Krasnoyarsk Territory, caused mainly by B. miyamotoi. The results of the study significantly expand the known geographic area of the ITBB-BM.
IN VITRO DIAGNOSIS FOR EBOLA VIRUS DISEASE. A COMPARISON OF CURRENT TECHNIQUES AND DIAGNOSTIC ASSAYS
Authors: Sementsova A. O. , Dedkov V. G. , Ternovoy V. A. , Chub E. V. , Pyankov S. A. , Agafonov A. P. , Maksyutov R. A. , Maleev V. V. , Popova A. Yu.
Published: September 2019

Ebola virus disease is dangerous viral infection, occurring in the form of hemorrhagic fever, characterized by acute clinical symptoms and high mortality rate due to multiple organ failure. Ebola virus natural foci are located in forested areas of the central and western parts of Africa. It was believed for many years, the incidence of Ebola virus disease has been sporadic and the burden of it is true only in endemic areas. However, the unprecedented Ebola epidemic caused by Zaire virus in 2013 — 2016, has significantly changed our understanding of this disease and the patterns of its distribution. We have also identified weaknesses in the organization of anti-epidemic measures, the effectiveness of which was not very effective at the onset of the epidemic, in particular due to weak development of in vitro diagnostics (IVD). However, during the elimination of the epidemic in West Africa, anti-epidemic system has been modified substantially, largely due to quickly developed IVD kits. This review is devoted to analysis of trends in IVD for Ebola virus disease based on the experience obtained in the course of the West-African epidemic in 2013 — 2016.
SCIENTIFIC AND METHODOLOGICAL SUPPORT FOR DEVELOPMENT OF PATHOGENIC MICROORGANISM COLLECTION OF VOLGOGRAD RESEARCH INSTITUTE FOR PLAGUE CONTROL
Authors: Molchanova E. V. , Ageeva N. P.
Published: September 2019

The review reflects the current state of the working in collection and presents the directions of its improvement within the framework of the carried out certification of the collection of pathogenic Burkholderia spp. of Volgograd Research Institute for Plague Control. The ways of modernization of existing methods of conservation, optimization of modern methods of phenotypic and molecular-genetic characteristics of strains of pathogenic microorganisms are considered, and an information cataloging system with the formation of a universal database is proposed.
DETERMINATION OF THE RABIES VIRUS-INFECTED VERO LINE CELL PORTION BY FLOW CYTOMETRY
Authors: Kravtsov A. L. , Generalov S. V. , Kozhevnikov V. A. , Gavrilova Yu. K. , Abramova E. G. , Kochkin A. V. , Nikiphorov A. K.
Published: September 2019

Aim. Experimental substantiation of possibility to determine the rabies virus-infected Vero cell line portion in culture by flow cytometry (FC) and FITC labeled diagnostic anti-rabies immunoglobulin (DAI), manufactured in Russia. Materials and methods. Fixation and permeabilization of Vero cells, infected by rabies virus strain «Moscow 3253», was carried out by means of Суtofix/Cytoperm reagent (BD Biosciences, USA) according the Vengatesan D. et al. method (2006) and then intracellular rabies antigen was stained by DAI. Percentage of infected cells was determined by FC in 24, 48 and 72 h and as well in 48 h when the cell cultures were infected with tenfold dilutions of virus-containing fluid from 10-1 to 10-8. Results. There was a significant increase in the percentage of infected cells on average from 30 to 70% in time interval from 24 to 48 h. With 1000-fold dilution of viral-containing fluid the FC method detected the 6,9±0,21% of infected cells in Vero cultures (P0,001, n=3). Conclusion. FC has proved to be a fast, sensitive and reliable method for determining the relative number of virus- infected Vero cells in cultures. The drug DAI had a sufficient activity for its effective use in the automated version of MFA based on the FC method. The use of FC is possible at various stages of anti-rabies drug production and control, and is also promising in terms of further improving of the rabies diagnosis.
IDENTIFICATION AND PHYSIOLOGICAL CHARACTERIZATION OF A CONSORTIUM OF HYDROCARBON-OXIDIZING BACTERIA OF OIL AND OIL PRODUCTS
Authors: Shapiro T. N. , Dolnikova G. A. , Nemtseva N. V. , Sandzhieva D. A. , Lobakova E. S.
Published: September 2019

A consortium of microorganisms were isolated from TC-1 fuel form, each member of which is capable of consistently degrade hydrocarbons’ different fractions. The 5 strains of hydrocarbon-oxidizing bacteria (PSB) were identified and isolated from TS-1 jet fuel. Their physiological and biochemical features are defined. All strains exhibit positive catalase activity. It is determined that all UOB strains, producing exogenous and endogenous surfactants, are capable to growth on media with different fraction of hydrocarbons. The study of these associations allows to create effective preparations for bioremediation in the elimination of accidental spills of oil and petroleum products.
GENETIC CHARACTERISTICS OF THE ADAPTIVE POTENTIAL OF BIFIDOBACTERIA IN THE BIOTOPE OF DISTAL HUMAN INTESTINE
Authors: Andryuschenko S. V. , Ivanova E. V. , Perunova N. B. , Bukharin O. V. , Bekpergenova A. V.
Published: September 2019

Aim. Determination of distinctive parameters of the B. bifidum and B. longum genomes, which characterizes their adaptive potential applied to distal intestine biotope of the human gut. Materials and methods. 5 strains of bifidobacteria have been used: B. bifidum ICIS-310, B. bifidum ICIS-643, B. bifidum ICIS-791, B. longum ICIS-505 (clinical isolates) и B. longum MC-42. Whole Genome Sequencing (WGS) has been performed by «MiSeq» DNA sequencer and «Nextera» DNA library preparation kit (Illumiina). Annotation and the primary analysis of known homologues gene content has been performed by RAST service (NMPDR). Results. B. bifidum ICIS-310 has not revealed lactose and galactose permease genes, that present in two other sequenced B. bifidum strains, but two exo-alph-sialidase genes has remained, as well as additional gene of DNA-methyltransferases family. Clinical isolates of B. longum has demonstrated a slightly more differences between each other: B. longum ICIS-505 strain contains more than 200 genes more than B. longum MC-42 reference strain, where are 29 genes - homologoues with known function. These genes are distributed uniformly by functional groups. Conclusion. Obtained data of genome analysis of the bifidobacteria reflect their specialization in occupied biotope and mutu-alistic reliability, determining dominance role of bifidoflora in human gut microsymbiocoenosis. Genome size, stability of signal census and predictability of reactions of the bifidobacteria allow to use them as a general model suitable for studying of symbiotic relations of human and his/her microbiota as well as construction of the experimental systems of intermicrobial interactions.
CERVICAL MUCUS NEUTROPHILS FUNCTIONAL STATUS INTERACTION WITH THE SPECIES COMPOSITION OF VAGINAL LACTOBACILLI IN PREGNANT WOMEN
Authors: Shishkova Yu. S. , Dolgushina V. F. , Grafova E. D. , Zavyalova S. A. , Kurnosenko I. V. , Evstigneeva N. P. , Gromakova K. G. , Kolesnikov O. L. , Chukichev A. V. , Dolgushin I. I.
Published: September 2019

Aim. Of the study is to assess cervical mucus neutrophils functional status depend on dominant lactobacillus strain in I trimester of pregnancy women. Material and methods. We definded 40 lactobacillus strains obtained from the genital tract of the pregnant women. We used mass spectrometry analysis to identify vaginal lactobacilli and evaluated biofilm formation. Also, we studied cervical mucus neutrophils viability, lysosomal activity, spontaneous and induced NBT test (Nitroblue Tetrazolium test), ability to absorb the latex particles. Isolated strains were divided into 3 groups depending on presence or absence of vaginal or cervical channel infectious diseases. Results. We identified 5 lactobacilli types: L. jensenii, L. mspatus, L. аcidophilus, L. gasseri, L. delbrueckii among 40 definded strains. We determined different intensity of phagocytosis depending on dominant strain in normocenosis. With the L. crispatus prevalence demonstrated the least intensity of phagocytosis compared with L. acidophilus and L.jensenii. Lactobacilli able to intensively produce biomatrix in condition of genital infection. Thus blocking antigenic potential and result in absence of NBT-reducing power of neutrophilic granulocytes and reduction antimicrobial protection of pregnant women genital tract mucosa. Conclusion. The indicators of neutrophils functional status depend on dominant lactobacilli strain in I trimester of pregnancy women.
MOLECULAR MECHANISMS OF BRUCELLA PERSISTENCE
Authors: Kulakov Yu. K.
Published: September 2019

Brucellosis is an infectious, especially dangerous zoonotic disease of agricultural and wild animals, from which it is transmitted to humans and characterized by a chronic course with disability of working-age patients. Bacteria of the genus Brucella are facultative intracellular pathogens capable of multiplying and persisting in the host’s immune cells with the development of chronic infection. The host-specific evolutionary mechanisms allow Brucella to hide and manipulate the systems of innate and acquired cellular immunity to achieve intracellular persistence. The review describes the molecular mechanisms that ensure the persistence of the causative agent of brucellosis. The evolution of Brucella species is associated with the adaptation of intracellular preservation and persistence in the formed granulomatous structures. Understanding the molecular mechanisms of Brucella persistence should be considered in programs for its control and elimination, and also allows the development of new effective tools for the prevention and treatment of brucellosis.
DISBIOSIS CORRECTION - THE BASIS OF REGENERATIVE MEDICINE
Authors: Mikhailova N. A. , Voevodin D. A. , Poddubikov A. V.
Published: September 2019

The effectiveness of regeneration is provided by normal over exchange and regulatory responses, so the regenerative therapy should be directed at identifying and addressing the causes of dismetabolk influences. Mikrobiocenosis is an integral part of holistic human organism takes part in realization of all metabolic reactions of the media. Correction of disbiosis helps restore regenerative processes, for the development of this direction is necessary to develop a new generation of probiotic preparations.
EPIDEMIOLOGICAL ASPECTS OF SEPSIS
Authors: Noskova O. A. , Anganova E. V. , Gvak G. V. , Savilov E. D.
Published: September 2019

Last decades a sepsis problem attracts the increased interest in the world community. In spite of definite achievements of modern fundamental and clinical medicine, sepsis as before is characterized by significant dissemination and high lethality. The problems of sepsis spread in various countries are discussed. It is shown that annually million cases of generalized purulent-septic infections are diagnosed. Steady sepsis increase is registered in industrially developed countries. Share of severe sepsis in pathology structure varies from 2 to 43% in different territories. Sepsis still remains among leading causes of human death being characterized by essential hospital lethality (from 30,6 to 80,4%). The patient categories belonging to high risk groups of sepsis development are shown. Special attention is directed to epidemiological manifestations of this pathological syndrome in pediatrics. Features of etiologic spectrum of the sepsis causative agents, increasing etiological importance of multi-resistant bacteria (Pseudomonas aeruginosa, Klebsiella pneumoniae, Acinetobacter spp., MRSA, VRE, etc.) are demonstrated. In consideration of clinical-epidemiological, social and economic significance of sepsis, studying of its epidemiological aspects is the major direction of activities for Public Health services.
REACTOGENICITY, SAFETY AND IMMUNOGENICITY OF DOMESTIC FLU-M INACTIVATED SPLIT INFLUENZA VACCINE FOR THE IMMUNIZATION OF ADULTS AGED BETWEEN 18 AND 60
Authors: Feldblyum I. V. , Subbotina K. A. , Novgorodova S. D. , Ignatev G. M. , Alyeva M. Kh. , Erofeeva M. K. , Orlovsky V. G. , Leneva I. A. , Melnikov S. Ya. , Kazakova E. V. , Nacharova E. P. , Trukhin V. P.
Published: September 2019

Aim. The research was aimed at evaluating the reactogenicity, safety and immunogenicity of Flu-M inactivated split influenza vaccine. Materials and methods. The reactogenicity, safety and immunogenicity of the drug were studied in the course of a multicenter, double blind, comparative, randomized clinical trial of immunized volunteers aged between 18 and 60 (comparator - Vaxigrip inactivated split vaccine for influenza prevention). Results. Domestic Flu-M inactivated split influenza vaccine manufactured by St. Petersburg Research Institute of Vaccines and Sera features favorable tolerability, high safety profile and adequate immunogenicity which is consistent with that of Vaxigrip influenza vaccine. Conclusion. Flu-M inactivated split influenza vaccine manufactured by St. Petersburg Research Institute of Vaccines and Sera can be recommended for authorization in the Russian Federation for the purpose of specific prophylaxis of influenza for adults aged between 18 and 60.
MELIOIDOSIS AND GLANDERS: CURRENT STATE AND ACTUAL ISSUES OF EPIDEMIOLOGICAL SURVEILLANCE
Authors: Zakharova I. B. , Toporkov A. V. , Viktorov D. V.
Published: September 2019

Burkholderia pseudomallei and Burkholderia mallei are etiologic agents of glanders and melioidosis, the particularly dangerous infections of animals and humans, and are attributed to potential agents of bioterrorism. The manifestation of diseases ranges from acute septicemia to chronic infection, any organs and tissues are affected, andtreatment requires long intravenous and oral antibiotic courses. The endemic zone of glanders and melioidosis covers spacious regions in the world, and the number of imported cases to temperate regions is constantly increasing. For the Russian Federation, glanders and melioidosis are «forgotten» and «unknown» infections, and this review presents current data on their distribution in the world, epidemiological aspects, and laboratory diagnosis features.