bluuebunny/crossref_metadata_2025 huggingface dataset:
Naturally derived Heme-Oxygenase 1 inducers attenuate inflammatory responses in human dendritic cells and T cells: relevance for psoriasis treatment
Authors: Campbell Nicole K. , Fitzgerald Hannah K. , Malara Anna , Hambly Roisin , Sweeney Cheryl M. , Kirby Brian , Fletcher Jean M. , Dunne Aisling
Published: July 2018

Psoriasis is a chronic autoimmune disease mediated by dysregulated immune responses in dendritic cells (DC) and T cells. The stress-response enzyme heme oxygenase-1 (HO-1) has been described as protective in animal models of psoriasis, however, implementation of HO-1-based therapies is hindered by the lack of clinically-suitable HO-1 inducers. The plant-derived polyphenols, carnosol and curcumin, have been identified as candidate HO-1 inducers however there has been little investigation into their effects on human immune cells. We demonstrate that treatment of human DC with these polyphenols limits DC maturation, reduces pro-inflammatory cytokine production, and prevents induction of allospecific T cell responses, in a manner partially dependent on carbon monoxide (CO). We also characterised their effects in ex-vivo psoriasis PBMC and report that curcumin, but not carnosol, strongly reduces T cell proliferation and cytokine poly-functionality, with reduced expression of psoriatic cytokines IFNγ, IL-17, GM-CSF and IL-22. This study therefore supports reports highlighting the therapeutic potential of curcumin in psoriasis by providing insight into its immunological effects on healthy human DC and psoriasis PBMC. We also demonstrate, for the first time, the anti-inflammatory effects of carnosol in human immune cells.
Microstructure characterization of BaSnO3 thin films on LaAlO3 and PrScO3 substrates from transmission electron microscopy
Authors: Yun Hwanhui , Ganguly Koustav , Postiglione William , Jalan Bharat , Leighton Chris , Mkhoyan K. Andre , Jeong Jong Seok
Published: July 2018

Detailed microstructure analysis of epitaxial thin films is a vital step towards understanding essential structure-property relationships. Here, a combination of transmission electron microscopy (TEM) techniques is utilized to determine in detail the microstructure of epitaxial La-doped BaSnO3 films grown on two different perovskite substrates: LaAlO3 and PrScO3. These BaSnO3 films are of high current interest due to outstanding electron mobility at ambient. The rotational disorder of low-angle grain boundaries, namely the in-plane twist and out-of-plane tilt, is visualized by conventional TEM under a two-beam condition, and the degree of twists in grains of such films is quantified by selected-area electron diffraction. The investigation of the atomic arrangement near the film-substrate interfaces, using high-resolution annular dark-field scanning TEM imaging, reveals that edge dislocations with a Burgers vector along [001] result in the out-of-plane tilt. It is shown that such TEM-based analyses provide detailed information about the microstructure of the films, which, when combined with complimentary high-resolution X-ray diffraction, yields a complete structural characterization of the films. In particular, stark differences in out-of-plane tilt on the two substrates are shown to result from differences in misfit dislocation densities at the interface, explaining a puzzling observation from X-ray diffraction.
Geometric compensation applied to image analysis of cell populations with morphological variability: a new role for a classical concept
Authors: Figueiredo Joana , Rodrigues Isabel , Ribeiro João , Fernandes Maria Sofia , Melo Soraia , Sousa Bárbara , Paredes Joana , Seruca Raquel , Sanches João M.
Published: July 2018

Immunofluorescence is the gold standard technique to determine the level and spatial distribution of fluorescent-tagged molecules. However, quantitative analysis of fluorescence microscopy images faces crucial challenges such as morphologic variability within cells. In this work, we developed an analytical strategy to deal with cell shape and size variability that is based on an elastic geometric alignment algorithm. Firstly, synthetic images mimicking cell populations with morphological variability were used to test and optimize the algorithm, under controlled conditions. We have computed expression profiles specifically assessing cell-cell interactions (IN profiles) and profiles focusing on the distribution of a marker throughout the intracellular space of single cells (RD profiles). To experimentally validate our analytical pipeline, we have used real images of cell cultures stained for E-cadherin, tubulin and a mitochondria dye, selected as prototypes of membrane, cytoplasmic and organelle-specific markers. The results demonstrated that our algorithm is able to generate a detailed quantitative report and a faithful representation of a large panel of molecules, distributed in distinct cellular compartments, independently of cell’s morphological features. This is a simple end-user method that can be widely explored in research and diagnostic labs to unravel protein regulation mechanisms or identify protein expression patterns associated with disease.
Nannochloropsis oceanica, a novel natural source of rumen-protected eicosapentaenoic acid (EPA) for ruminants
Authors: Alves Susana P. , Mendonça Sofia H. , Silva Joana L. , Bessa Rui J. B.
Published: July 2018

We hypothesize that whole microalga biomass is a natural rumen-protected source of eicosapentaenoic acid (EPA, 20:5n-3) for ruminants. To test our hypothesis, we studied the ruminal biohydrogenation of EPA from two microalgae, Nannochloropsis oceanica and Phaeodactylum tricornutum using in vitro incubations with rumen fluid. A total mixed ration was incubated with: no EPA (control), EPA as free-fatty acid, N. oceanica spray-dried (SD), N. oceanica freeze-dried (FD), or P. tricornutum FD. The kinetics of EPA disappearance and of products formed during the 24 hours of incubation were evaluated, and complemented by deuterated-EPA incubation. Results showed that EPA metabolism from the N. oceanica was remarkably reduced compared with the P. tricornutum and free-EPA, and this reduction was even more effective with the N. oceanica FD. Our data also indicates that neither feed dry matter disappearance nor rumen microbial markers (branched-chain fatty acids and dimethyl acetals) were affected by EPA-sources. We reported for the first time the kinetics of EPA biohydrogenation class products and the unequivocal formation of 20:0 from EPA. Overall, N. oceanica shows a strong potential to be used as a natural dietary source of EPA to ruminants, nevertheless further studies are needed to verify its protection in vivo.
Synchronisation of Arabidopsis flowering time and whole-plant senescence in seasonal environments
Authors: Miryeganeh Matin , Yamaguchi Masaki , Kudoh Hiroshi
Published: July 2018

Synchronisation of flowering phenology has often been observed between individuals within plant species. We expected that a critical role of flowering-time control under natural conditions is a phenological synchronisation. However, no studies have quantified the level of synchronisation of reproductive timing relative to germination timing under natural conditions. In a sequential seeding experiment (SSE) in which we manipulated the germination timing of Arabidopsis thaliana accessions, we developed a quantification index to evaluate reproductive synchrony in annual plants. In the SSE, we identified a novel phenomenon of reproductive synchrony: senescence synchrony. The role of vernalisation in realising flowering synchrony between plants of different ages under natural conditions was demonstrated by synchronisation and de-synchronisation of flowering initiation in vernalisation-sensitive and less-vernalisation-sensitive accessions, respectively. We also observed up-regulation of senescence-related genes at corresponding times. The approach we developed in this study provides a set of concepts and procedures that can be used to study reproductive synchrony experimentally under natural conditions.
Routine hemodialysis induces a decline in plasma magnesium concentration in most patients: a prospective observational cohort study
Authors: Leenders Nicoline H. J. , van Ittersum Frans J. , Hoekstra Tiny , Hoenderop Joost G. J. , Vervloet Marc G.
Published: July 2018

In hemodialysis patients, lower plasma magnesium (Mg) concentrations are associated with a higher overall and cardiovascular mortality. The optimal concentration appears to be above the reference range for the healthy population. Plasma Mg is not routinely measured after hemodialysis. Aim of this study was to determine the effect of routine hemodialysis on plasma Mg. Plasma Mg was measured in duplicate before (Mgpre) and after (Mgpost) dialysis in 6 consecutive hemodialysis sessions of 34 patients using a fixed 0.50 mmol/L dialysate Mg concentration. Mean Mgpre was 0.88 mmol/L (±0.14) and mean Mgpost was statistically significantly lower: mean intra-dialytic decline 0.10 mmol/L (95%-CI 0.06–0.13). A 0.10 mmol/L higher Mgpre was associated with a 0.03 mmol/L higher Mgpost (95%-CI 0.024–0.037). At a Mgpre of 0.74 mmol/L, Mgpost equalled Mgpre. There was an intra-dialytic decline of plasma Mg at higher Mgpre values and an increase at lower Mgpre values. In conclusion, in the majority of the hemodialysis patients, Mgpre concentrations are in the reference range of the healthy population, which may be too low for hemodialysis patients. Routine hemodialysis with the widely used 0.50 mmol/L dialysate Mg concentration, further declines magnesium in the majority of patients. Current dialysate Mg concentrations may be too low.
Irradiation-based design of mechanically resistant microstructures tuned via multiscale phase-field modeling
Authors: Demange Gilles , Dépinoy Sylvain , Lunéville Laurence , Simeone David , Pontikis Vassilis
Published: July 2018

We present a multi-scale phase field modeling of stationary microstructures produced under 1 MeV krypton ion irradiation in a phase separating concentrated solid solution of silver and copper. We show that the mixture reaches ultimately a stationary micro-structural state made of phase domains with composition and size distribution mapped to the values of the incident flux of particles and of the temperature, variables that help defining a non equilibrium phase-diagram for the irradiated alloy. The modeling predicts the formation of diverse microstructures likely connected to spinodal hardening, thus opening the perspective of the on-purpose tuning of mechanically resistant microstructures and the preparation of metastable alloys with mechanical properties improved by comparison to counterparts obtained via classical thermo-mechanical treatments.
Tuning the micromorphology and exposed facets of MnOx promotes methyl ethyl ketone low-temperature abatement: boosting oxygen activation and electron transmission
Authors: Jian Yanfei , Ma Mudi , Chen Changwei , Liu Chao , Yu Yanke , Hao Zhengping , He Chi
Published: July 2018

MnOx nanowires with highly exposed {101} facets of Mn3O4 possess excellent low-temperature activity and stability for methyl ethyl ketone destruction.
Alkyltin clusters: the less symmetric Keggin isomers
Authors: Hutchison Danielle C. , Stern Rebecca D. , Olsen Morgan R. , Zakharov Lev N. , Persson Kristin A. , Nyman May
Published: July 2018

Sn4+ forms the only Keggin ion family that favors the lowest symmetry β and γ isomers.
Enhanced solar cell stability by hygroscopic polymer passivation of metal halide perovskite thin film
Authors: Kim Min , Motti Silvia G. , Sorrentino Roberto , Petrozza Annamaria
Published: July 2018

A hygroscopic polymer thin film successfully encapsulates an organic–inorganic halide perovskite layer, showing enhanced stability of the solar cell operating in a humid atmosphere.
Engineering copper nanoparticles synthesized on the surface of carbon nanotubes for anti-microbial and anti-biofilm applications
Authors: Seo Youngmin , Hwang Jangsun , Lee Eunwon , Kim Young Jin , Lee Kyungwoo , Park Chanhwi , Choi Yonghyun , Jeon Hojeong , Choi Jonghoon
Published: July 2018

Copper nanoparticles synthesized on carbon nanotubes can not only remove bacteria but also biofilm, which is difficult to be destroyed by conventional methods.
Acoustically-mediated intracellular delivery
Authors: Ramesan Shwathy , Rezk Amgad R. , Dekiwadia Chaitali , Cortez-Jugo Christina , Yeo Leslie Y.
Published: July 2018

High frequency sound waves enable enhanced cytosolic transport of molecular cargo into cells without the formation of pores.
Hierarchically stimuli-responsive nanovectors for improved tumor penetration and programed tumor therapy
Authors: Liu Junjie , Guo Xingming , Luo Zhong , Zhang Jixi , Li Menghuan , Cai Kaiyong
Published: July 2018

Poor drug delivery to solid tumors remains a great challenge for effective antitumor therapy.
Highly efficient luminescent side-chain polymers with short-spacer attached tetraphenylethylene AIEgens via RAFT polymerization capable of naked eye explosive detection
Authors: Li Qian , Li Xiao , Wu Zhongying , Sun Yuhao , Fang Jianglin , Chen Dongzhong
Published: July 2018

The fluorescence quantum yield of side-chain AIE polymers was remarkably promoted just by shortening the linking spacer.
A flexible polymer–nanoparticle hybrid material containing triazole-based Fe(ii) with spin crossover properties for magneto-optical applications
Authors: Voisin Hugo , Aimé Carole , Vallée Anne , Coradin Thibaud , Roux Cécile
Published: July 2018

Centimeter-scale materials exhibiting solid state-like spin-crossover properties and easy handling under ambient conditions are obtained that preserve their thermochromic behavior over months.
Simple, selective and fast detection of acrylamide based on glutathione S-transferase
Authors: Bucur Madalina-Petruta , Bucur Bogdan , Radu Gabriel-Lucian
Published: July 2018

Acrylamide (AA) is a toxic compound formed in thermally prepared foods by Maillard reaction.
Amino acid ionic liquids as catalysts in a solvent-free Morita–Baylis–Hillman reaction
Authors: Pereira Mathias Prado , Souza Martins Rafaela de , de Oliveira Marcone Augusto Leal , Bombonato Fernanda Irene
Published: July 2018

In this work, we describe the preparation of ten amino acid ionic liquids (AAILs). The AAILs were used as catalysts in a Morita–Baylis–Hillman (MBH) reaction. The MBH adducts were obtained from moderate to good yields and in their racemic form.
Solid-state dye-sensitized solar cells based on Zn1−xSnxO nanocomposite photoanodes
Authors: El-Shazly Ayat Nasr , Shalan Ahmed Esmail , Rashad Mohamed Mohamed , Abdel-Aal Elsayed Ali , Ibrahim Ibrahim Ahmed , El-Shahat Mohamed F.
Published: July 2018

Solid-state dye-sensitized solar cells (ss-DSSCs) comprising Sn2+-substituted ZnO nanopowder were purposefully tailored via a co-precipitation method.
Electrodeposition of tin nanowires from a dichloromethane based electrolyte
Authors: Lodge Andrew W. , Hasan Mahboba M. , Bartlett Philip N. , Beanland Richard , Hector Andrew L. , Kashtiban Reza J. , Levason William , Reid Gillian , Sloan Jeremy , Smith David C. , Zhang Wenjian
Published: July 2018

Tin was electrodeposited from a dichloromethane-based electrolyte at ambient temperature into gold coated anodic alumina membranes with nanoscale pores.
A novel near-infrared fluorescent probe for highly selective recognition of hydrogen sulfide and imaging in living cells
Authors: Zhong Keli , Deng Longlong , Zhao Jie , Yan Xiaomei , Sun Tong , Li Jianrong , Tang Lijun
Published: July 2018

A novel “off–on” fluorescent probe (L) for H2S detection with NIR emission and imaging H2S in living cells.
Full-color tunable photoluminescent carbon dots based on oil/water interfacial synthesis and their applications
Authors: Guo Hongchong , You Bo , Zhao Shuyan , Wang Yihao , Sun Gang , Bai Yujie , Shi Lei
Published: July 2018

Full-color tunable photoluminescent (PL) carbon dots (CDs) are fabricated through a one-step catalytic-oxidation process at the oil/water interface.
Two decades of advances in diterpenoid alkaloids with cytotoxicity activities
Authors: Liang Xiaoxia , Gao Yingying , Luan Shangxian
Published: July 2018

The important pharmacological activities and structural complexity of diterpenoid alkaloids have long stimulated strong scientific interest; some of these naturally abundant compounds have been reported to be highly promising for treating cancer.
A porous Co–Ru@C shell as a bifunctional catalyst for lithium–oxygen batteries
Authors: Chen Xiang , Zhang Xiuhui , Chen Chunguang , Huang Tao , Yu Aishui
Published: July 2018

We use SiO2 as a template and dopamine as a carbon source to synthesize a hollow C shell, and we load Co and Ru nanoparticles onto it to obtain a Co–Ru@C shell composite.
Mechanisms of catalytic reduction of CO2 with heme and nonheme metal complexes
Authors: Fukuzumi Shunichi , Lee Yong-Min , Ahn Hyun S. , Nam Wonwoo
Published: July 2018

This critical review discusses the thermal and photocatalytic mechanisms of one-, two-, four-, six- and eight-electron reduction of CO2 with metal complex catalysts.
Anthraquinone thin-film electrodes for reversible CO2 capture and release
Authors: Wielend Dominik , Apaydin Dogukan Hazar , Sariciftci Niyazi Serdar
Published: July 2018

We report reversible electrochemical capture and release of carbon dioxide using the well-known dye precursor and industrial catalyst anthraquinone.
Mesoporous TiN microspheres as an efficient polysulfide barrier for lithium–sulfur batteries
Authors: Qi Bin , Zhao Xiaosen , Wang Shaogang , Chen Ke , Wei Yingjin , Chen Gang , Gao Yu , Zhang Dong , Sun Zhenhua , Li Feng
Published: July 2018

A separator modified with mesoporous titanium nitride (MTN) microspheres acting as a highly effective polysulfide blocking layer for Li–S batteries.
Ultrahigh energy density due to self-growing double dielectric layers at a titanium/sol–gel-derived amorphous aluminium oxide interface
Authors: Su Zhen , Yao Manwen , Yao Xi
Published: July 2018

Pursuing high-energy-density dielectric materials is desirable for the growing demands of energy storage for dielectric capacitors in modern electric power systems.
Deficit in the epidermal barrier induces toxicity and translocation of PEG modified graphene oxide in nematodes
Authors: Zhao Li , Kong Jingting , Krasteva Natalia , Wang Dayong
Published: July 2018

Our data provide the molecular basis for the role of epidermal barrier against toxicity and translocation of nanomaterials in organisms.
Research on hydrogen-induced cracking behavior of normalized pipeline steel
Authors: Zhang Hai , Li Shaopo , Ding Wenhua , Hao Ning
Published: July 2018

Hydrogen sulfide corrosion test was used to test the hydrogen-induced cracking sensitivity of the normalized BNS pipeline steel. The microstructure and morphology of hydrogen induced crack(HIC) of the normalized BNS pipeline steel after hydrogen sulfide corrosion test were observed with optical microscopy(OM), scanning electron microscopy(SEM). Combined with electron probe microanalyzer(EPMA) and hardness test, the hydrogen-induced cracking behavior of BNS pipeline steel was studied from the aspects of microstructure, crack morphology, center segregation and harness. The results showed that the pearlite band with high hardness caused by center segregation of C and Mn was the main crack initiation and propagation path for the long-size and linear shape hydrogen induced crack at the center of plate thickness, and the type of crack propagation was transgranular. Some tiny hydrogen induced crack nucleated from the small calcium-aluminate inclusion and the tiny hydrogen induced crack would not propagate to form long-size cracks with no suitable propagation path existing around the inclusion.
Design and study of distributed photo-voltaic power generation system in sewage treatment plant
Authors: Mingsheng Zhang , Hesong Wu , Wenkui Wan , Peilei Feng
Published: July 2018

With the rapid development of the global economy, solar photovoltaic power generation has attracted wide attention all over the world. With the rapid development of all kinds of new energy in the world, photovoltaic power generation has a huge international market and broad prospects for development. This paper analyzes the feasibility of the distributed photovoltaic power generation system in this city, based on the actual situation of a photovoltaic power generation project in a certain place. Through the type selection of a variety of solar cell groups, component installation, operation mode selection, and inverter selection, Therefore, a more reasonable PV module and electrical part(The design and study of the first part, the two part of the electrical system and the lightning protection measures) are designed. The research shows that the comprehensive value of photovoltaic power is very high, and the distributed photovoltaic power station can be built in the areas where the geographical conditions are suitable and the light is sufficient. After the completion of the above power generation projects, the local solar energy resources will be fundamentally transformed into economic advantages.
Research of Firing Control Model of Multi-stage Synchronous Induction Coil Launcher
Authors: Zhao Keyi , Yuan Xichao , Xiang Hongjun , Zhang Qian , Qingao Lv
Published: July 2018

The problem of firing control is a critical technology of multi-stage synchronous induction coil launcher (MSSICL). Operation with high launch efficiency of MSSICL mainly depends on each stage driving coil energized when the armature moves on the proper location. In order to solve the problem of firing control for MSSICL, the firing control model of MSSICL is built based on the dynamically simulative model and the firing control criterion. The dynamically simulative model includes the voltage equations of driving circuit and the kinematic equations. The solved method is provided. In addition, the factors that influence the solved precision of the firing control model of MSSICL are analyzed.
Construction of Corpus in Artificial Intelligence Age
Authors: Hong Changchun
Published: July 2018

As a kind of revolutionary technology, artificial intelligence marked an explosive transformation in many fields of study. Nowadays, much of the translation work used to be done by human has been undertaken by machines. The construction of corpus is a crucial step leading to successful machine translation. The paper aims to exploring the mode of corpus construction from the perspective of information mining, information retrieval and information processing. The retrieval system uses web crawlers to collect network information and automatic tagging technology to index the collected information, then applies corresponding language processing techniques to achieve correspondence between two languages and form an index database. In the age of artificial intelligence, machines can keep a track of many users’ searches, queries, so as to record, extract as well as to feed back on different translations to build a new corpus. In this way, machine translation is improving in its scope and accuracy in translation with the goal to take up the tedious work of human translation as well as to increase the speed and reduce the cost of it.
A temperature compensation method of measuring frequency for cylindrical vibratory gyroscope in frequency split trimming
Authors: Hu Youwang , Zeng Kai , Wang Haofang , Liang Chang , Zhou Yongping , Sun Xiaoyan
Published: July 2018

In the process of frequency split trimming, the fluctuation of environmental temperature causes a deviation in measuring the natural frequency, which is bigger than the request trimming accuracy. Therefore, a measuring method with temperature compensation is proposed to reduce this impact. Firstly, the relationship between natural frequency of resonator and environmental temperature are studied by experiments. Furthermore, the corresponding model of temperature compensation is proposed, which is written into the measuring unit to calculate the compensated frequency. The frequencies before and after temperature compensation are measured to prove the correctness of this method. At last, a real time measuring experiment is accomplished to verify the feasibility in frequency split trimming. Results show that the effect of temperature on the natural frequency reduced significantly with the temperature compensation measuring method. The proposed method is applicable for other types of vibratory gyroscope in precision frequency measurement.
Kinematic Simulation and Analysis of Globoidal Indexing Cam
Authors: Xinzhe Hu , Huize Feng , Huiying Tang , Xueming Hong , Jiankai Fan
Published: July 2018

As an important mechanism with intermittent motion, the globoidal indexing cam is always a research hot in the mechanical fields. The working profile of globoidal indexing cam is extremely complicated and undevelopable, which make it quite difficult to be protracted by the conventional drafting method. Aiming at this problem, the working curvilinear equation of the intermittent motion of an indexing cam is derived based on the RPY (Roll-Pitch-Yaw) coordinate transformation method. The 3D model based on the curvilinear equation is built by the Creo2.0 modeling software. The virtual prototype is established based on the ADAMS software, while the kinematics simulation is implemented. The success of virtual simulation verifies the correctness of curvilinear equation. The numerical results, presented and discussed in the paper, indicate that the proposed model is feasible to foresee the kinematic behaviour of an actual system.
Study on Stability of Round Tunnel Surrounding Rock
Authors: Yan NIU , Yafei Ji , Zhao Wang
Published: July 2018

Tunnel excavation will lead to the immediate surrounding rock unloading caused by the surrounding rock stress release, the stability of the surrounding rock have a certain impact. In this paper, finite element software ANSYS and finite difference software FLAC3D are used to simulate the excavation and lining process of circular tunnel. The influence of excavation on the rock stability around circular tunnel is analyzed, and the effect of applying lining on the stability of surrounding rock is analyzed. Evaluation criteria selection hole displacement, stress and plastic area of three factors.
Application of Irregular Flowing Water Construction in Architectural Garden Project Management
Authors: Xiuyun Li
Published: July 2018

Irregular flowing water construction is the common way of the construction of the garden project. On the basis of elaborating the form of flow water construction, the paper focuses on the general method of irregular flowing water construction, especially in the calculation of activity-on-arrow network, the garden project also needs to be introduced in the form of virtual work, in order to achieve the clear logic of the construction process and continuous construction.
Study on the Potential of Hyperaccumulator Leersia hexandra swartz to Repair Chromium Contamination of Heavy Metals
Authors: Lin Zhiyi , Lin Hua , Wang Yian , Yuan Yuhang
Published: July 2018

In order to control the environmental pollution caused by chromium better, this paper summaries the study about the accumulation charac-teristics, tolerance mechanism, detoxification mechanism and enrichment efficiency of chromium in Leersia hexandra swartz in recent years. It concluded that Leersia hexandra swartz has considerable potential for remediation of polluted environment, especially large-area, low-concentration polluted water, and prospects of its future research direction.
Seismic Performance Analysis of Continuous Rigid Frame Bridges in Expressway under Non-linear Interactions of Soil-Piles
Authors: Yongbing FENG
Published: July 2018

Taking the three-span pre-stressed concrete continuous rigid frame bridge as an engineering example, MIDAS Civil was utilized to establish a spatial finite element model and the interaction between pile foundation and the soil was simulated by equivalent soil spring. In addition to analyzing shearing force, bending moment and stress of the primary beam's characteristic section under different loads, a response spectrum method and time history analysis were adopted to conduct seismic response analysis respectively. In this case, performance of the bridge could be comprehensively evaluated. Relevant analysis results indicate that internal force of the large-span pre-stressed concrete continuous rigid frame bridge is mainly induced by gravity and pre-stress of the structure; section stresses of the primary beam satisfy the corresponding specification and structural safety can be achieved in a state of operation. Moreover, computed results obtained by the response spectrum method is more conservative than those of the time history analysis. In terms of continuous rigid frame bridge, different seismic directions should be taken into consideration during structural seismic analysis at different construction stages.
Design and Implementation of Training Management Software for General Simulation Training System
Authors: Xiang Wei , Chunsheng Sun , Yanhong Dong
Published: July 2018

The common simulation training system realizes the common design of hardware and software, and also increases the difficulty of group training management. In order to solve the problem of flexible group training of multiple training modes for general simulation training system of a certain type of naval combat system, this paper puts forward a formal description method of group training resources and group training programs based on an in-depth analysis of training management requirements. Design and implementation of a general simulation training system training management software.
Reconstruction Method of Electrical Capacitance Tomography Based on Wavelet Fusion
Authors: Lei Zhou , Jiangbao Yan , Feng Zhu , Xiangyu Tan , Lifeng Zhang
Published: July 2018

The accuracy of reconstructed images of Electrical Capacitance Tomography (ECT) is a bottleneck concerning the successful application of ECT. An image data fusion algorithm based on wavelet transform was proposed in this paper to improve the accuracy of reconstructed images. First, reconstructed images were obtained using conjugate gradient least square algorithm and Landweber iterative algorithm, respectively. Secondly, reconstructed images were decomposed by wavelet. After that, the approximate component was processed according to the weighted average fusion rule. The detail component was processed according to the maximum fusion rule of absolute value. Finally, the new reconstructed images were obtained by wavelet reconstruction. Simulation and static experimental results showed that the reconstructed images with higher accuracy can be obtained after fusion and the artefacts were decreased obviously.
Knowledge Evolution of Complex Agent Networks
Authors: Yeqing Zhao
Published: July 2018

In order to study the interaction between structure change of social network and knowledge propagation, this paper proposes a complex agent network model to discover the inner rule and restraining factors of the knowledge diffusion in the network system. The agents take advantage of different social radius to form acquaintance networks based on the theory of social circles in the knowledge propagation network model, and the dynamic evolution process of knowledge network is realized according the defined rules of knowledge communication. Simulation results show that this model based on social circle theory can better realize the characteristics of the actual social network than the traditional network model established before, at the same time the social radius of knowledge agent for knowledge dissemination in knowledge network has the obvious effect. It can narrow the knowledge gap for the knowledge agents in social network and good social relation network can be developed.
Optimization Acceleration Integral Method Based on Power Spectrum Estimation
Authors: Guo Ruijie , Ye Shengbo , Ji Yicai
Published: July 2018

Due to the excellent performance of the frequency domain integration method, it is widely used for acceleration integral calculations. However, the frequency-domain integration needs to select the effective integration frequency band to achieve its optimal integration performance. This paper proposes the method with power spectrum density (PSD) estimation to realize the optimization integral of the acceleration signal. By analysing the power spectrum density of the acceleration signal, the optimal low frequency cut-off frequency is obtained. Combined with frequency domain integration algorithm, it can effectively remove low-frequency noise and improve integral accuracy. Then, the novel algorithm tested by an experiment platform with a vibration bench. Experiment results show that this algorithm can adaptively select the low-frequency cut-off frequency and realize frequency domain integration optimization and the integration error is controlled within ±0.2mm.
Rehabilitation mit einem demenzkranken Angehörigen: Ergebnisse einer längsschnittlichen Beobachtungstudie
Authors: Deck Ruth , Bussmann Marie-Luise , Simons Janine , Al-Hashimy Synan , Stark Michael
Published: July 2018

Hintergrund Pflegende Angehörige von Demenzerkrankten sind erheblichen Belastungen ausgesetzt. Ein multimodales Rehabilitationskonzept, welches die stationäre Behandlung von pflegerisch tätigen Angehörigen zusammen mit den Demenzkranken ermöglicht, wurde evaluiert.

Methodik Schriftliche Befragung von 121 pflegenden Angehörigen zu 3 Messzeitpunkten. Erfasst wurden Parameter der subjektiven Gesundheit sowie die Inanspruchnahme von pflegerischen und psychosozialen Unterstützungsangeboten im Verlauf.

Ergebnisse Es zeigen sich deutliche gesundheitliche Verbesserungen am Ende der Rehabilitation. Sechs Monate nach der Rehabilitation gehen die Effekte zurück, erreichen jedoch bei keinem der erfassten Merkmale den Ausgangswert vor der Reha.

Schlussfolgerungen Das multimodale Rehabilitationskonzept zeigt zumindest in Teilbereichen nachhaltige Effekte. Diese sowie die hohe Zufriedenheit der Teilnehmer sprechen dafür, dass sich das Rehabilitationskonzept bewährt und mit einem hohen gesundheitlichen Gewinn verbunden ist.
Dissociation of Platelet Activation and Spontaneous Myocardial Ischemia in Unstable Angina
Authors: Vejar Margarita , Fragasso Gabriele , Hackett David , Lipkin David P , Maseri Attilio , Born Gustav V R , Ciabattoni Giovanni , Patrono Carlo
Published: July 2018

A dynamic thrombotic process, coronary spasm or both can be responsible for recurrent episodes of transient reduction of coronary blood flow in unstable angina. We have investigated the temporal relationship between episodic platelet activation, as detected by increased urinary excretion of 11-dehydro-TXB2, and spontaneous myocardial ischemia, assessed by continuous electrocardiographic monitoring and recording in 21 patients with unstable angina pectoris. In order to validate measurements of metabolite excretion as a reflection of intracoronary platelet activation, we have also performed repeated urine sampling from 8 patients undergoing PTCA and from 6 patients with peripheral vascular disease. The latter showed a 16% coefficient of variation in 3 consecutive 8-h urine samples. 11-dehydro-TXB2 increased significantly, by up to 15-fold, in the 2.5- to 5.0-h urine collection encompassing PTCA and decreased by > 50% during the following 2-h period. Patients with unstable angina were characterized by episodic increases (>2 SD of controls) in metabolite excretion, in successive 6-8 h specimens. Paired measurements of 11-dehydro-TXB2 and 2, 3-dinor-TXB2 in 15 urine samples did not reveal evidence of altered metabolic disposition of endogenously released TXB2. A total of 125 ECG ischemic episodes were recorded, of which 64% asymptomatic. We have compared these biochemical and ECG changes in patients randomized to i. v. low-dose aspirin or i.v. isosorbide dinitrate and oral diltiazem. Twenty-five of 56 (i.e. 45%) urine samples obtained in aspirin-free periods showed increased metabolite excretion as compared to 15 of 88 (i.e. 17%) samples collected during aspirin. Of the former, only 3 episodes of enhanced 11-dehydro-TXB2 excretion were associated with ST-segment changes, 7 with chest pain, and 15 with no ECG or clinical changes. Metabolite excretion was approximately 70% lower during aspirin administration than during coronary dilators. However, despite > 95% suppression of platelet cyclooxygenase activity, as monitored ex vivo, incomplete suppression of in vivo TXB2 biosynthesis was occasionally seen during low-dose aspirin therapy. We conclude that in unstable angina, episodic platelet activation is infrequently associated with spontaneous myocardial ischemia. Although the two events may represent functional expressions of the same coronary lesion, they are likely to be triggered by independent mechanisms through different mediators.
Development of MDL 28,050, a Small Stable Antithrombin Agent Based on a Functional Domain of the Leech Protein, Hirudin
Authors: Krstenansky John L , Broersma Robert J , Owen Thomas J , Payne Marguerite H , Yates Mark T , Mao Simon J T
Published: July 2018

MDL 28,050 is a decapeptide antithrombin agent that inhibits a-thrombin-induced fibrin clot formation by binding to a non-catalytic site on α-thromhin. It is the result of chemical and structural optimization of a functional domain of the leech anticoagulant, hirudin. In contrast to the contention that the polyanionic nature of this C-terminal functional domain governs its interaction with α-thrombin, systematic study of this region has shown the importance of the lipophilic residues for providing the functionality necessary foi potent binding to a-thrombin. The development of MDL 28,050 and other effective antithrombin agents are outlined through the description of the structure-activity relationships (SAR) for these peptides. These peptides are effective in a variety of in vitro and in vivo models of thrombosis.
Observations on the Cell Biology of Tissue Factor in Endothelial Cells
Authors: Andoh Kiyoshi , Pettersen Kjell Sverre , Filion -Myklebust Christiane , Prydz Hans
Published: July 2018

Human umbilical vein endothelial cells (HUVEC) are inducible for tissue factor (TF) activity in culture. Based on experiments using ECGF (4–20 µg/ml) with heparin (90 µg/ml), we obtained the followingresults: 1) In confluent HUVEC cultures, ECGF had essentially no influence on the levels of inducible TF. 2) In growing HUVEC cultures, ECGF reduced the TF response shortly after seeding but full response was regained when cells were kept confluent for 2–3 days. 3) Although secondary cultures responded best to TF induction in the absence of ECGF, the response was essentially equal over at least 8 passages in the prcgcncc of ECGF 1) Of total cellular TF induced in HUVEC, about 25% was available on the surface, and less than 4% was released with theshed plasma membrane vesicles. The proportion of total TF a>ctivity available on the surface of mtact cells wasnot influenced by the presence of ECGF 5) T½, for the decay of TF activity induced was 8.3—9.5 h, whereas in HUVEC when protein synthesis was blocked afterIF induction a T½ of about 30 h was found.
Immunoelectrophoretic Analysis of Membrane Glycoproteins in Cultured Human Endothelial Cells
Authors: Børsum Tone
Published: July 2018

Human endothelial cells isolated from umbilical cordswere solubilized in Triton X-100 and examined by crossedimmunoelec-trophoresis using rabbit antiserum against endothelial cells. Endogenous labelling of the endothelialcell proteins with 14Cmannose followed by crossed immunoelectrophoresis and autoradiography revealed about 10 immunoprecipitates. Four of these endothelial cell glycoproteins were labelled by lactoperoxidase catalyzed iodination and thus were surface located. Three of the surface located glycoproteins showed reduced electrophoretic mobility after incubation of the endothelial cells with neuraminidase and were therefore sialoglycoproteins. Amphiphilicity of endothelial cell glycoproteins was studied by crossed hydrophobic interaction immunoelectrophoresis with phenyl-Sepharose in the intermediate gel. Amphiphilic proteins also show increasing electrophoretic migration velocity with decreasing concentration of Triton X-100 in the first dimension gels. Five of the endothelial cell glycoproteins were shown to be amphiphilic using these two techniques.

Two monoclonal antibodies against the platelet glycoprotein complex Ilb-IIIa and glycoprotein IlIa, respectively, reacted with the same precipitate of endothelial cells. When a polyclonal antibody against the platelet glycoprotein complex Ilb-IIIa was incorporated into the intermediate gel the position of two endothelial cell precipitates were lowered. One of these was a sialoglycoprotein.
Monitoring Heparin Therapy: Relationships between the Activated Partial Thromboplastin Time and Heparin Assays Based on Ex-Vivo Heparin Samples
Authors: Bessekaar A M H P van den , Meeuwisse-Braun J , Bertina R M
Published: July 2018

Five different APTT reagents, two amidolytic anti-ITa assays, one amidoiytic anti-Xa assay, and one coagulometric anti-Xa/ anti-IIa assay were used to assess the effect of heparin in patients treated for venous thromboembolic disease. Good correlations were observed between lug-transformed APYE> determined with the various reagents (correlation coefficients: 0.92-0.96).

Nevertheless there were important differences in the slopes of the lines of relationship between the APTT reagents.

Good correlations were observed between the anti-Xa and anti-IIa assay results (correlation coefficients: 0.92-0.97). However, the amidolytic anti-Xa activity was significantly higher (p <0.001) than the two amidolytic anti-IIa activities.

Less good correlations were observed between the log-transformed APTTs and the anti-Xa or anti-IIa activities (correlation coefficients: 0.64-0.78). The correlations were improved by transforming the APTT into APTT-ratio, i.e. the ratio of the patient’s APTT to the same patient’s APTT after removal of heparin from the plasma sample by means of ECTEOLA-cellulose treatment. The correlation coefficients of log (AFTT-ratio) with anti-Xa or anti-IIa ranged from 0.76 to 0.87.

For both APTT and amidolytic heparin assay, the response to in vitro heparin was different from the response to ex vivo heparin.

Therefore, equivalent therapeutic ranges should be assessed by using ex vivo samples rather than in vitro heparin. Because of the response differences between the APTT reagents, it is not adequate to define a therapeutic range for heparin therapy without specification of the reagent.
The Effect of Combined Factor XI Deficiency with von Willebrand Factor Abnormalities on Haemorrhagic Diathesis
Authors: Tavori Shulamith , Brenner Benjamin , Tatarsky llana
Published: July 2018

To account for the lack of correlation between the level of factor XI (FXI) in deficient patients and haemorrhagic manifestations, we correlated the prevalence of combined FXI and von Willebrand’s factor (vWF) deficiency in 212 FXI-deficient patients. Fifty-four patients had a combined FXI and vWF deficiency: 16 patients had severe and 38 patients had mild FXT deficiency. In a group of 28 patients with comparably mild FXI deficiency, 14 bleeders had significantly lower mean vWF, Ag, ristocetin cofactor and ristocetin induced platelet aggregation than 14 non-bleeders selected on the basis of comparable FXI levels. These findings suggest that the combination of FXI and vWF deficiency is common and may affect the bleeding tendency in mild FXI deficiency.
Platelet Modulation of Neutrophil Superoxide Anion Production
Authors: Moon Dudley G , Zee Hoyte van Der , Weston Lisa K , Gudewicz Paul W , Fenton II John W , Kaplan John E
Published: July 2018

The effect of platelets on polymorphonuclear leukocytes (PMN) O−
2 production was examined using autologous sheep and human cell systems. Coincubation of sheep platelets with sheep PMNs in the absence of thrombin resulted in a significant inhibition in basal PMN O−
2 production. The platelet-derived inhibitory activity was released into the medium and could be destroyed by adenosine deaminase suggesting that the inhibitor was adenosine. Addition of alpha-thrombin or platelet activating factor (PAF) enhanced PMN O−
2 production but only when platelets were present. The enhancement of O−
2 production in response to thrombin was dependent upon the thrombin concentration and the platelet-PMN ratio. With a platelet:PMN ratio of 30:1, addition of 10 nM thrombin to sheep cells resulted in a 5-fold increase in O−
2 production, whereas addition of 10 nM PAF caused a 2-fold increase in O−
2. Addition of thrombin or PAF to either PMNs or platelets by themselves did not initiate an increase in O−
2 generation. The response of human cells was similar except that both thrombin and PAF triggered a 2-fold increase in PMN O−
2 production in the presence of platelets. The platelet-derived enhancement activity was not released into the medium and was not blocked by WEB 2086, NDGA, ETYA, aspirin or adenosine deaminase. The enhancement effect appeared to be localized to the platelet membrane and we believe requires platelet-PMN contact.
The Effect of Different Anaesthetic Techniques on the Incidence of Thrombosis following Total Hip Replacement
Authors: Hoek Jaap A , Henny Ch Pieter , Knipscheer Haye C , ten Cate Hugo , Nurmohamed Michael T , ten Cate Jan W
Published: July 2018

We performed a retrospective analysis on the influence of three types of anaesthesia on the incidence of deep vein thrombosis (DVT) following total hip replacement (THR) in consecutive patients randomized to either the low molecular weight heparinoid Otg 10172 (97 patients), of placebo (99 patients). Ninety patients were operated under epidural anaesthesia, 77 patients under psoas compartment block with additional inhalation anaesthesia, and 29 patients under general anaesthesia. DVT assessment was performed by bilateral venography between days 8 and 12 postoperatively. The overall incidence of DVT in the 196 patients was 37% in the epidural anaesthasia group, 35% in the psoas compartment block group, and 36% in the general anaesthesia group. Although the incidence of DVT in patients randomuedto placebo was similar in the two anaesthesia groups (53%), there was an important reduction of the occurrence of proximal DVT by the heparinoid in the psoas compartment block group (from 20 to 0%), compared to the epidural anaesthesia group (from 27 to l8%) (p <0.0061). Significant y more minor wound hematomas occurred in the psoas compartment block group as compared to the epidural anaesthesia group (p <0.05). Synergrsm of thrombin generation inhibition by the heparinoid and inhibition of plateletlggregation at the damaged vessel wall, by high local concentrations of bupivacaine in the psoas compartment block technique, is proposed as a possible mechanism behind this observation.
Further Evidence that Glycoprotein IIb-IIIa Mediates Platelet Spreading on Subendothelium
Authors: Weiss Harvey J , Turitto Vincet T , Baumgartner Hans R
Published: July 2018

In order to explore further the mechanism by which glycoprotein GPIIb-IIIa promotes platelet vessel wall interaction, platelet adhesion to subendothelium was studied in an annular chamber in which subendothelium from rabbit aorta was exposed at a shear rate of 2,600 s−1 to blood from patients with thrombasthenia. Perfusions were conducted for each of 5 exposure times (1 ,2,3, 5 and 10 min), and the percent surface coverage of the vessel segment with platelets in the contact (C) and spread (S) stage was determined. Increased values of platelet contact (C) were obtained in thrombasthenia at all exposure times; this finding is consistent with a defect in platelet spreadirg, based on a previously described kinetic model of platelet attachment to subendothelium. According to this model of attachment, increased values of platelet contact (C) at a single exposure time may be indicative of either a defect in spreading (S) or initial contact (C), but multiple exposures will result in increased contact only for defects which are related to defectiye platelet spreading (s).

The results obtained over a broad range of exposure times provide more conclusive evidence that GPIIb-IIIa mediates platelet spreading than those previously obtained at single exposure times.