bluuebunny/crossref_metadata_2025 huggingface dataset:
Hotspot Regions of Quantitative Trait Loci and Candidate Genes for Ear-Related Traits in Maize: A Literature Review
Authors: Zhang Xingjie , Sun Jiachen , Zhang Yudong , Li Jinfeng , Liu Meichen , Li Linzhuo , Li Shaoxiong , Wang Tingzhao , Shaw Ranjan Kumar , Jiang Fuyan , Fan Xingming
Published: December 2023

In this study, hotspot regions, QTL clusters, and candidate genes for eight ear-related traits of maize (ear length, ear diameter, kernel row number, kernel number per row, kernel length, kernel width, kernel thickness, and 100-kernel weight) were summarized and analyzed over the past three decades. This review aims to (1) comprehensively summarize and analyze previous studies on QTLs associated with these eight ear-related traits and identify hotspot bin regions located on maize chromosomes and key candidate genes associated with the ear-related traits and (2) compile major and stable QTLs and QTL clusters from various mapping populations and mapping methods and techniques providing valuable insights for fine mapping, gene cloning, and breeding for high-yield and high-quality maize. Previous research has demonstrated that QTLs for ear-related traits are distributed across all ten chromosomes in maize, and the phenotypic variation explained by a single QTL ranged from 0.40% to 36.76%. In total, 23 QTL hotspot bins for ear-related traits were identified across all ten chromosomes. The most prominent hotspot region is bin 4.08 on chromosome 4 with 15 QTLs related to eight ear-related traits. Additionally, this study identified 48 candidate genes associated with ear-related traits. Out of these, five have been cloned and validated, while twenty-eight candidate genes located in the QTL hotspots were defined by this study. This review offers a deeper understanding of the advancements in QTL mapping and the identification of key candidates associated with eight ear-related traits. These insights will undoubtedly assist maize breeders in formulating strategies to develop higher-yield maize varieties, contributing to global food security.
Musculoskeletal, Functional and Performance Impairment in Female Overhead Athletes with a Previous Shoulder Injury
Authors: Alonso-Muñoz María Belén , Calvache-Mateo Andrés , Martín-Núñez Javier , López-López Laura , Navas-Otero Alba , Heredia-Ciuró Alejandro , Valenza Marie Carmen
Published: December 2023

Background: Shoulder injuries are substantial problems in overhead athletes, and more studies are necessary to deepen the knowledge on this type of injury. The objective of this study was to compare the overall function and performance of female overhead athletes with and without a previous history of shoulder injuries. Methods: In this cross-sectional study, female overhead athletes with and without a previous shoulder injury were included. Muscular impairment, the stability of the shoulder, strength, scapular dyskinesia, functionality and sports performance were evaluated. A total of 50 females were included. Results: There were significant differences in strength (p = 0.046) and stability (p = 0.039) between groups, with a poorer score in the group with a history of shoulder injury. Regarding scapular dyskinesia, significant differences were also observed between groups (p = 0.048), with higher levels of dyskinesia in the group with previous shoulder injury. Also, muscular impairment showed significant differences between groups for the three muscles evaluated (p < 0.005). Additionally, the group without a previous shoulder injury presented with a significantly greater score in functionality (p = 0.046) and sports performance (p = 0.004). Conclusion: In conclusion, previous shoulder injuries are an important factor to take into account in female overhead athletes. Players with a history of shoulder injury present clinical impairments during the game, leading to poorer functional status and performance in sport.
The Relationship between Self-Rated Health and Physical Fitness in Polish Youth
Authors: Kochman Maciej , Kielar Aleksandra , Kasprzak Marta , Maruszczak Krystian , Kasperek Wojciech
Published: December 2023

Self-rated health (SRH) is a tool for assessing a population’s health across the lifetime, and seems to be a dynamic assessment of current health status and a strong predictor of cardiovascular disease and mortality, whereas insufficient levels of physical fitness in adolescence are a significant health problem and may contribute to the development of many disorders in adulthood. In this cross-sectional study, we attempted to assess the relationship between SRH and the physical fitness of adolescents. Two hundred and thirty-five adolescents (eighty-five boys and one hundred and fifty girls) aged 16–17 were recruited for this study. The study procedures included a short author questionnaire and physical fitness assessment (Zuchora’s Physical Fitness Index). Boys declared better health states and reported less frequent morbidity of seasonal diseases (p < 0.05). No differences were found in physical fitness, the incidence of postural disorders, lower limb malalignments, foot deformities, sleep duration, and perception of physical activity on physical condition and well-being in boys and girls (p > 0.05). The comparison of physical fitness levels in adolescents with different SRH, sleep duration, perceptions of physical activity on physical condition and well-being also showed significant differences (p < 0.05). Positive SRH and perception of physical activity on physical condition and well-being, proper sleep duration (7–8 h/night), and a lack of foot deformities are associated with a better physical fitness in adolescents. Physical fitness seems to be a good predictor of SRH only in Polish boys, but not girls and the entire population. The lack of significance in the entire population could be attributed to the substantial number of girls in the study group. Despite that, it is recommended to encourage adolescents to engage in regular exercises, sleep routines and healthy lifestyles. Further research should be based on a more representative group, with a comparable number of girls and boys in the study group and potential confounders, but also they should be focused on SRH predictors to improve SRH in Polish girls.
Electronic Nicotine Delivery Systems Use and Periodontal Health—Findings from the Population Assessment of Tobacco and Health Study
Authors: Mohajeri Amir , Lipsky Martin S. , Hegde Rachana , Chiang Jody , Hung Man
Published: December 2023

(1) Background: Electronic nicotine delivery systems (ENDSs) are rapidly increasing in the U.S., however, information about their long-term risks and benefits remains limited. This study examined the relationship between ENDS use and periodontal health among U.S. adults. (2) Methods: Data came from 33,822 adults who participated in the 2016–2018 wave of the Population Assessment of Tobacco and Health (PATH) study. Inclusion criteria were adults without a history of cigarette smoking or diabetes. Logistic regression analysis was performed to estimate the associations between ENDS use and a history of periodontal disease, with multivariable logistic regression adjusting for factors associated with poor oral health. (3) Results: Of the study participants, 2321 were never ENDS users, 38 were regular ENDS users, and 512 were non-regular ENDS users. Compared to never ENDS users, regular ENDS users had higher odds of poor periodontal health including bone loss around teeth. Regular ENDS use was also independently associated with higher odds of poor oral health compared to non-regular ENDS users. (4) Conclusions: This study suggests an association between ENDS use and increased risk of periodontal health issues in the United States. These findings align with previous research linking ENDS use to poor oral health.
Data Augmentation with Cross-Modal Variational Autoencoders (DACMVA) for Cancer Survival Prediction
Authors: Rajaram Sara , Mitchell Cassie S.
Published: December 2023

The ability to translate Generative Adversarial Networks (GANs) and Variational Autoencoders (VAEs) into different modalities and data types is essential to improve Deep Learning (DL) for predictive medicine. This work presents DACMVA, a novel framework to conduct data augmentation in a cross-modal dataset by translating between modalities and oversampling imputations of missing data. DACMVA was inspired by previous work on the alignment of latent spaces in Autoencoders. DACMVA is a DL data augmentation pipeline that improves the performance in a downstream prediction task. The unique DACMVA framework leverages a cross-modal loss to improve the imputation quality and employs training strategies to enable regularized latent spaces. Oversampling of augmented data is integrated into the prediction training. It is empirically demonstrated that the new DACMVA framework is effective in the often-neglected scenario of DL training on tabular data with continuous labels. Specifically, DACMVA is applied towards cancer survival prediction on tabular gene expression data where there is a portion of missing data in a given modality. DACMVA significantly (p << 0.001, one-sided Wilcoxon signed-rank test) outperformed the non-augmented baseline and competing augmentation methods with varying percentages of missing data (4%, 90%, 95% missing). As such, DACMVA provides significant performance improvements, even in very-low-data regimes, over existing state-of-the-art methods, including TDImpute and oversampling alone.
Two Isomers of a Novel Ag(I) Complex with Pyrazole-Type Ligand—Synthesis, Structural, Thermal, and Antioxidative Characterization
Authors: Radnović Nikola D. , Štetin Nađa , Radanović Mirjana M. , Borišev Ivana Đ. , Rodić Marko V. , Jaćimović Željko K. , Barta Holló Berta
Published: December 2023

The synthesis of the first Ag(I) complexes with ethyl-5-amino-1-methyl-1H-pyrazole-4-carboxylate (L) is presented. The reaction of AgClO4 with the ligand in a molar ratio of 1:1 gives a bis(ligand) complex [AgL2]ClO4 (1) in the presence of 4-formylbenzonitrile, monoperiodic polymer {[AgL2]ClO4}n (2). Characterization involved IR spectroscopy, conductometric measurements, thermogravimetric analysis, antioxidant tests, powder, and single crystal X-ray diffraction. Structural analysis revealed ligand coordination in a monodentate manner through the nitrogen atom of the pyrazole ring in both complexes. Complex 1 displayed a linear coordination environment for Ag(I), whereas, in complex 2, square-planar coordination was achieved with the additional involvement of two oxygen atoms from bridging perchlorate anions. Notably, the thermal properties of both isomers are found to be nearly identical. The significant antioxidant activity of the isomer with a reverse-oriented pyrazole-type ligand suggests its potential relevance in biological studies.
An Approach for Using a Tensor-Based Method for Mobility-User Pattern Determining
Authors: Ashaev Ivan P. , Safiullin Ildar A. , Gaysin Artur K. , Nadeev Adel F. , Korobkov Alexey A.
Published: December 2023

Modern mobile networks exhibit a complex heterogeneous structure. To enhance the Quality of Service (QoS) in these networks, intelligent control mechanisms should be implemented. These functions are based on the processing of large amounts of data and feature extraction. One such feature is information about user mobility. However, directly determining user mobility remains challenging. To address this issue, this study proposes an approach based on multi-linear data processing. The user mobility is proposed to determine, using the multi-linear data, about the changing of the Signal-to-Interference-plus-Noise-Ratio (SINR). SINR varies individually for each user over time, relative to the network’s base stations. It is natural to represent these data as a tensor. A tensor-based preprocessing step employing Canonical Polyadic Decomposition (CPD) is proposed to extract user mobility information and reduce the data volume. In the next step, using the DBSCAN algorithm, users are clustered according to their mobility patterns. Subsequently, users are clustered based on their mobility patterns using the DBSCAN algorithm. The proposed approach is evaluated utilizing data from Network Simulator 3 (NS-3), which simulates a portion of the mobile network. The results of processing these data using the proposed method demonstrate superior performance in determining user mobility.
Change over Five Years in Important Measures of Methodological Quality and Reporting in Randomized Cardiovascular Clinical Trials
Authors: Baasan Odgerel , Freihat Omar , Nagy Dávid U. , Lohner Szimonetta
Published: December 2023

Objectives: The aim of our current study was to analyze whether the use of important measures of methodological quality and reporting of randomized clinical trials published in the field of cardiovascular disease research haschanged over time. A furtheraim was to investigate whether there was an improvement over time in the ability of these trials to provide a good estimate of the true intervention effect. Methods: We conducted two searches in the Cochrane Central Register of Controlled Trials (CENTAL) database to identify randomized cardiovascular clinical trials published in either 2012 or 2017. Randomized clinical trials (RCTs) trials in cardiovascular disease research with adult participants were eligible to be included. We randomly selected 250 RCTs for publication years 2012 and 2017. Trial characteristics, data on measures of methodological quality, and reporting were extracted and the risk of bias for each trial was assessed. Results: As compared to 2012, in 2017 there were significant improvements in the reporting of the presence of a data monitoring committee (42.0% in 2017 compared to 34.4% in 2012; p < 0.001), and a positive change in registering randomized cardiovascular disease research in clinical trial registries (78.4% in 2017 compared to 68.9% in 2012; p = 0.03). We also observed that significantly more RCTs reported sample size calculation (60.4% in 2017 compared to 49.6% in 2012; p < 0.01) in 2017 as compared to 2012. RCTs in 2017 were more likely to have a low overall risk of bias (RoB) than in 2012 (29.2% in 2017 compared to 21.2% in 2012; p < 0.01). However, fewer 2017 RCTs were rated low (50.8% compared to 65.6%; p < 0.001) risk for blinding of participants and personnel, for blinding of outcome assessors (82.4% compared to 90.8%; p < 0.001), and selective outcome reporting (62.8% compared to 80.0%; <0.001). Conclusions: As compared to 2012, in 2017 there were significant improvements in some, but not all, the important measures of methodological quality. Although more trials in the field of cardiovascular disease research had a lower overall RoB in 2017, the improvement over time was not consistently perceived in all RoB domains.
A Retrospective Study of Trifluridine/Tipiracil with Fruquintinib in Patients with Chemorefractory Metastatic Colorectal Cancer
Authors: Zou Jiayun , Wang Yuanyuan , Xu Jiayu , Li Jinna , Wang Tianzhuo , Zhang Ying , Bai Yibo
Published: December 2023

Introduction: Trifluridine/tipiracil (TAS-102) and fruquintinib are novel antitumor agents for patients with refractory metastatic colorectal cancer (mCRC). We conducted a retrospective study to explore the clinical efficacy and drug toxicities of combination therapy with TAS-102 and fruquintinib in real-life clinical practice. Methods: Between March 2021 and February 2023, patients at two different centers with mCRC who failed two or more lines of prior therapy and received TAS-102 in combination with fruquintinib were recruited. Results: In total, 32 mCRC patients were included in the analysis. The objective response rate (ORR) and the disease control rate (DCR) were 9.4% and 75%. The median progression-free survival (PFS) and overall survival (OS) were 6.3 (95% CI: 5.3–7.3) and 13.5 (95% CI: 9.5–17.5) months, respectively. Patients without liver metastasis or peritoneal metastasis obtained better median PFS (7.1 m vs. 5.6 m, p = 0.03 and 6.3 m vs. 3.4 m, p = 0.04), and OS (15.2 m vs. 10.4 m, p = 0.01 and 13.6 m vs. 7.1 m, p = 0.03), respectively. Other clinicopathological features, including age, tumor site, KRAS status, dosage of fruquintinib, and treatment line, did not affect the clinical efficacy of TAS-102 combined with fruquintinib. The most common grade three–four toxicities were neutropenia (46.9%), anemia (21.9%), diarrhea (15.6%), nausea (12.5%), and hand–foot syndrome rash (12.5%). Conclusions: Our results suggest that TAS-102 combined with fruquintinib has promising clinical efficacy and manageable safety for refractory mCRC patients in a real-world clinical setting. Further prospective trials are warranted to confirm our results.
Prostate Artery Embolization via Distal Transradial Artery Access in a 100-Year-Old Patient
Authors: Yeh Shauh-Der , Weng Yu-Shiou , Lin Chun-Yu
Published: December 2023

Benign prostatic obstruction (BPH) is a common disease in males and surgical treatment is the gold standard for this symptomatic disease. Prostate artery embolization (PAE) is one of the emerging therapies which aims to minimize the lower urinary tract symptoms (LUTS) of BPH and the volume of enlarged prostates. We reported here a case of 100-year-old man with 90 cm3 prostate and severe symptoms secondary to BPH, who underwent a successful PAE through distal transradial access without any complications. The patient was satisfied with this treatment and no symptoms recurred after PAE. This demonstrated that PAE was a safe and effective treatment for BPH and was recommended for elderly/non-surgical candidates.
Two Approaches to Estimate the Shapley Value for Convex Partially Defined Games
Authors: Masuya Satoshi
Published: December 2023

In the classical approach of von Neumann and Morgenstern to cooperative games, it was assumed that the worth of all coalitions must be given. However, in real-world problems, the worth of some coalitions may be unknown. Therefore, in this study, we consider the Shapley value for convex partially defined games using two approaches. Firstly, we introduce a polytope that includes the set of Shapley values that can be obtained from a given convex partially defined game and select one rational value in some sense from the set. The elements of this polytope are said to be the Shapley payoff vectors. Secondly, we obtain the set of Shapley values that can be obtained from a given convex partially defined game and select one rational value in some sense from the set. Moreover, we axiomatize the proposed two values.
Bayesian Estimation of a New Pareto-Type Distribution Based on Mixed Gibbs Sampling Algorithm
Authors: Li Fanqun , Wei Shanran , Zhao Mingtao
Published: December 2023

In this paper, based on the mixed Gibbs sampling algorithm, a Bayesian estimation procedure is proposed for a new Pareto-type distribution in the case of complete and type II censored samples. Simulation studies show that the proposed method is consistently superior to the maximize likelihood estimation in the context of small samples. Also, an analysis of some real data is provided to test the Bayesian estimation.
Algorithmic Approach for a Unique Definition of the Next-Generation Matrix
Authors: Avram Florin , Adenane Rim , Basnarkov Lasko , Johnston Matthew D.
Published: December 2023

The basic reproduction number R0 is a concept which originated in population dynamics, mathematical epidemiology, and ecology and is closely related to the mean number of children in branching processes (reflecting the fact that the phenomena of interest are well approximated via branching processes, at their inception). Despite the very extensive literature around R0 for deterministic epidemic models, we believe there are still aspects which are not fully understood. Foremost is the fact that R0 is not a function of the original ODE model, unless we also include in it a certain (F,V) gradient decomposition, which is not unique. This is related to the specification of the “infected compartments”, which is also not unique. A second interesting question is whether the extinction probabilities of the natural continuous time Markovian chain approximation of an ODE model around boundary points (disease-free equilibrium and invasion points) are also related to the (F,V) gradient decomposition. We offer below several new contributions to the literature: (1) A universal algorithmic definition of a (F,V) gradient decomposition (and hence of the resulting R0). (2) A fixed point equation for the extinction probabilities of a stochastic model associated to a deterministic ODE model, which may be expressed in terms of the (F,V) decomposition. Last but not least, we offer Mathematica scripts and implement them for a large variety of examples, which illustrate that our recipe offers always reasonable results, but that sometimes other reasonable (F,V) decompositions are available as well.
Genome-Wide Association (GWAS) Applied to Carcass and Meat Traits of Nellore Cattle
Authors: Reis Hugo Borges Dos , Carvalho Minos Esperândio , Espigolan Rafael , Poleti Mirele Daiana , Ambrizi Dewison Ricardo , Berton Mariana Piatto , Ferraz José Bento Sterman , de Mattos Oliveira Elisângela Chicaroni , Eler Joanir Pereira
Published: December 2023

The meat market has enormous importance for the world economy, and the quality of the product offered to the consumer is fundamental for the success of the sector. In this study, we analyzed a database which contained information on 2470 animals from a commercial farm in the state of São Paulo, Brazil. Of this total, 2181 animals were genotyped, using 777,962 single-nucleotide polymorphisms (SNPs). After quality control analysis, 468,321 SNPs provided information on the number of genotyped animals. Genome-wide association analyses (GWAS) were performed for the characteristics of the rib eye area (REA), subcutaneous fat thickness (SFT), shear force at 7 days’ ageing (SF7), and intramuscular fat (IMF), with the aid of the single-step genomic best linear unbiased prediction (ssGBLUP) method, with the purpose of identifying possible genomic windows (~1 Mb) responsible for explaining at least 0.5% of the genetic variance of the traits under analysis (≥0.5%). These genomic regions were used in a gene search and enrichment analyses using MeSH terms. The distributed heritability coefficients were 0.14, 0.20, 0.18, and 0.21 for REA, SFT, SF7, and IMF, respectively. The GWAS results indicated significant genomic windows for the traits of interest in a total of 17 chromosomes. Enrichment analyses showed the following significant terms (FDR ≤ 0.05) associated with the characteristics under study: for the REA, heat stress disorders and life cycle stages; for SFT, insulin and nonesterified fatty acids; for SF7, apoptosis and heat shock proteins (HSP27); and for IMF, metalloproteinase 2. In addition, KEGG (Kyoto encyclopedia of genes and genomes) enrichment analysis allowed us to highlight important metabolic pathways related to the studied phenotypes, such as the growth hormone synthesis, insulin-signaling, fatty acid metabolism, and ABC transporter pathways. The results obtained provide a better understanding of the molecular processes involved in the expression of the studied characteristics and may contribute to the design of selection strategies and future studies aimed at improving the productivity of Nellore cattle.
S100B Secretion in Astrocytes, Unlike C6 Glioma Cells, Is Downregulated by Lactate
Authors: Selistre Nicholas Guerini , Rodrigues Leticia , Federhen Barbara Carolina , Gayger-Dias Vitor , Taday Jéssica , Wartchow Krista Mineia , Gonçalves Carlos-Alberto
Published: December 2023

S100B is a calcium-binding protein produced and secreted by astrocytes in response to various extracellular stimuli. C6 glioma cells are a lineage commonly employed for astroglial studies due to the expression of astrocyte specific markers and behavior. However, in high-glucose medium, C6 S100B secretion increases, in contrast to the trend in primary astrocyte cultures. Additionally, S100B secretion decreases due to fluorocitrate (FC), a Krebs cycle inhibitor, highlighting a connection between S100B and metabolism. Herein, we investigate the impact of FC on S100B secretion in primary astrocyte cultures, acute hippocampal slices and C6 glioma cells, as well as lactate mediation. Our results demonstrated that C6 responded similarly to astrocytes in various parameters, despite the decrease in S100B secretion, which was inversely observed in astrocytes and slices. Furthermore, FC inversely altered extracellular lactate in both models, suggesting a role for lactate in S100B secretion. This was reinforced by a decrease in S100B secretion in hippocampal slices treated with lactate and its agonist, but not in C6 cells, despite HCAR1 expression. Our findings indicate that extracellular lactate mediates the decrease in S100B secretion in astrocytes exposed to FC. They also emphasize the differences in C6 glioma cells regarding energetic metabolism. The proposed mechanism via HCAR1 provides further compelling evidence of the relationship between S100B and glucose metabolism.
Metagenomic Insights into the Taxonomic and Functional Features of Traditional Fermented Milk Products from Russia
Authors: Elcheninov Alexander G. , Zayulina Kseniya S. , Klyukina Alexandra A. , Kremneva Mariia K. , Kublanov Ilya V. , Kochetkova Tatiana V.
Published: December 2023

Fermented milk products (FMPs) contain probiotics that are live bacteria considered to be beneficial to human health due to the production of various bioactive molecules. In this study, nine artisanal FMPs (kefir, ayran, khurunga, shubat, two cottage cheeses, bryndza, khuruud and suluguni-like cheese) from different regions of Russia were characterized using metagenomics. A metagenomic sequencing of ayran, khurunga, shubat, khuruud and suluguni-like cheese was performed for the first time. The taxonomic profiling of metagenomic reads revealed that Lactococcus species, such as Lc. lactis and Lc. cremoris prevailed in khuruud, bryndza, one sample of cottage cheese and khurunga. The latter one together with suluguni-like cheese microbiome was dominated by bacteria, affiliated to Lactobacillus helveticus (32–35%). In addition, a high proportion of sequences belonging to the genera Lactobacillus, Lactococcus and Streptococcus but not classified at the species level were found in the suluguni-like cheese. Lactobacillus delbrueckii, as well as Streptococcus thermophilus constituted the majority in another cottage cheese, kefir and ayran metagenomes. The microbiome of shubat, produced from camel’s milk, was significantly distinctive, and Lentilactobacillus kefiri, Lactobacillus kefiranofaciens and Bifidobacterium mongoliense represented the dominant components (42, 7.4 and 5.6%, respectively). In total, 78 metagenome-assembled genomes with a completeness ≥ 50.2% and a contamination ≤ 8.5% were recovered: 61 genomes were assigned to the Enterococcaceae, Lactobacillaceae and Streptococcaceae families (the Lactobacillales order within Firmicutes), 4 to Bifidobacteriaceae (the Actinobacteriota phylum) and 2 to Acetobacteraceae (the Proteobacteria phylum). A metagenomic analysis revealed numerous genes, from 161 to 1301 in different products, encoding glycoside hydrolases and glycosyltransferases predicted to participate in lactose, alpha-glucans and peptidoglycan hydrolysis as well as exopolysaccharides synthesis. A large number of secondary metabolite biosynthetic gene clusters, such as lanthipeptides, unclassified bacteriocins, nonribosomal peptides and polyketide synthases were also detected. Finally, the genes involved in the synthesis of bioactive compounds like β-lactones, terpenes and furans, nontypical for fermented milk products, were also found. The metagenomes of kefir, ayran and shubat was shown to contain either no or a very low count of antibiotic resistance genes. Altogether, our results show that traditional indigenous fermented products are a promising source of novel probiotic bacteria with beneficial properties for medical and food industries.
Mineral Chemistry of Pyrochlore Supergroup Minerals as Records of Nb Mineralization Processes in NYF-Type Pegmatites: A Case Study of the Emeishan Large Igneous Province, SW China
Authors: Yin Rong , Sun Xiaoming , Wang Shengwei , Wu Bin
Published: December 2023

Alkaline igneous rocks have become a potentially important source of Nb, except for the carbonatites. The metallogenetic mechanism of Nb during the magmatic-hydrothermal evolution of alkaline rocks remains ambiguous. From the perspective of Nb minerals, the mineral chemistry of pyrochlore supergroup minerals provides the mineralogical evidence for indicating the respective contributions of magma and hydrothermal fluids to Nb mineralization. In the Miyi County of the Panzhihua-Xichang (Pan-Xi) area, the central zone of the Permian Emeishan large igneous province (ELIP) in SW China, hundreds of Nb-Y-F mineralized pegmatites (NYF-type) are exposed. This study conducted in situ mineral chemistry analyses on four types of pyrochlores to elucidate the Nb mineralization process. Both Pcl-I and Pcl-II exhibit well-developed oscillatory zoning (OZ), representing magmatic pyrochlore formed through disequilibrium crystallization in an oscillatory environment. Pcl-III, with a homogeneous and less variable composition, is also considered of magmatic origin due to its coherent chemical evolution with Pcl-II. Pcl-IV is considered of hydrothermal origin based on its irregular zoning texture, extremely high Na2O contents, and compositional gap compared with magmatic types. The decrease in TiO2 contents, coupled with the increase in Na2O, F, and Nb2O5 contents from Pcl-I to Pcl-III and from the core to the rim of zoned Pcl-II, indicates that fractional crystallization facilitates the crystallization of albite and the enrichment of volatiles, as well as the precipitation of Nb from the early to late stages. During the magmatic-hydrothermal transition stage, the reductive, Na- and F-enriched fluid transports Nb more effectively, leading to further Nb enrichment in pyrochlore. Consequently, there are two-stage Nb mineralization processes associated with the magmatic-hydrothermal evolution in the Miyi pegmatite, with the primary magmatic ore assemblages being the dominant Nb mineralization, which may be a general model for the mineralization of NYF-type pegmatites.
Zircon U-Pb-Hf Isotopes and Whole-Rock Geochemistry of the “Kulumudi Formation” from the Laofengkou Area (West Junggar): Implications of the Construction of a Juvenile Arc in the Junggar–Balkhash Ocean
Authors: Liu Bo , Hou Lin-Xiao , Xu Yan , Ju Nan , Ma Jing-Xuan , Xie Zhi-Hao , Hong Yang-Bai-He
Published: December 2023

The properties of ancient magmatic arcs are crucial for understanding the tectonic evolution of the Central Asian Orogenic Belt. The Middle Devonian Kulumudi Formation in the Laofengkou area of West Junggar lacks accurate chronological data constraints, which hampers the knowledge of the nature of the Late Paleozoic magmatic arcs in the West Junggar and circum-Balkhash areas. In this contribution, samples of pyroclastic rocks and sedimentary rocks were collected from the volcano–sedimentary strata of the Kulumudi Formation. Petrography, zircon U-Pb-Hf isotopic analysis and whole-rock geochemistry were carried out to constrain the age and the tectonic setting of the Kulumudi Formation. The zircon U-Pb age of the lithic crystal tuff from the Kulumudi Formation on the northeast side of the Alemale Mountains was 386 ± 2 Ma, accurately indicating that this rock unit formed during the Middle Devonian. However, the fine sandstone near the Huojierte Mongolian Township, originally assigned as the “Kulumudi Formation”, yielded a maximum depositional age of 341 ± 3 Ma. Combined with the stratigraphic contact, this rock unit was redefined to belong to the Lower Carboniferous Jiangbasitao Formation. According to the whole-rock geochemistry study, the lithic crystal tuff of the Kulumudi Formation was characterized as medium potassium–calc–alkaline series rock, which is relatively enriched in light rare earth elements and large ion lithophile elements (i.e., Rb, Ba, K) and depleted in high-field-strength elements (i.e., Nb, Ta, Ti), showing similar geochemical characteristics to the volcanic arc rocks. By contrast, the fine sandstone from the Jiangbasitao Formation had Al2O3/SiO2 (0.25–0.29) and K2O/Na2O (1.29–1.72) ratios close to those derived from the continental arc and active continental margin and was characterized as part of the continental arc field in the La-Th-Sc and Th-Sc-Zr/10 tectonic discrimination diagrams. Zircon Hf isotope analysis showed that the εHf(t) values of the Kulumudi Formation were +5.6–+12.8, and those of the Jiangbasitao Formation were +11.43–+15.48, both of which show highly positive juvenile characteristics. The above data indicate that the Kulumudi Formation and Jiangbasitao Formation both formed in a juvenile arc setting with ocean–continent subduction. Combined with the previous work, it was concluded that the southward subduction of the ocean basin represented by the Darbut–Karamay ophiolitic mélanges beneath the newly accreted arc crustal segments produced a juvenile arc with positive Hf isotope characteristics.
Cobalt Encapsulated in Nitrogen-Doped Graphite-like Shells as Efficient Catalyst for Selective Oxidation of Arylalkanes
Authors: Li Shuo , Ali Shafqat , Zuhra Zareen , Shen Huahuai , Qiu Jiaxiang , Zeng Yanbin , Zheng Ke , Wang Xiaoxia , Xie Guanqun , Ding Shujiang
Published: December 2023

Selective oxidation of ethylbenzene to acetophenne is an important process in both organic synthesis and fine chemicals diligence. The cobalt-based catalysts combined with nitrogen-doped carbon have received great attention in ethylbenzene (EB) oxidation. Here, a series of cobalt catalysts with metallic cobalt nanoparticles (NPs) encapsulated in nitrogen-doped graphite-like carbon shells (Co@NC) have been constructed through the one-pot pyrolysis method in the presence of different nitrogen-containing compounds (urea, dicyandiamide and melamine), and their catalytic performance in solvent-free oxidation of EB with tert-butyl hydrogen peroxide (TBHP) as an oxidant was investigated. Under optimized conditions, the UCo@NC (urea as nitrogen source) could afford 95.2% conversion of EB and 96.0% selectivity to acetophenone, and the substrate scalability was remarkable. Kinetics show that UCo@NC contributes to EB oxidation with an apparent activation energy of 32.3 kJ/mol. The synergistic effect between metallic cobalt NPs and nitrogen-doped graphite-like carbon layers was obviously observed and, especially, the graphitic N species plays a key role during the oxidation reaction. The structure–performance relationship illustrated that EB oxidation was a free radical reaction through 1-phenylethanol as an intermediate, and the possible reaction mechanistic has been proposed.
Synthetic Approaches to Piperazine-Containing Drugs Approved by FDA in the Period of 2011–2023
Authors: Romanelli Maria Novella , Braconi Laura , Gabellini Alessio , Manetti Dina , Marotta Giambattista , Teodori Elisabetta
Published: December 2023

The piperazine moiety is often found in drugs or in bioactive molecules. This widespread presence is due to different possible roles depending on the position in the molecule and on the therapeutic class, but it also depends on the chemical reactivity of piperazine-based synthons, which facilitate its insertion into the molecule. In this paper, we take into consideration the piperazine-containing drugs approved by the Food and Drug Administration between January 2011 and June 2023, and the synthetic methodologies used to prepare the compounds in the discovery and process chemistry are reviewed.
Hexagonal Boron Nitride as Filler for Silica-Based Elastomer Nanocomposites
Authors: Magaletti Federica , Prioglio Gea , Giese Ulrich , Barbera Vincenzina , Galimberti Maurizio
Published: December 2023

Two-dimensional hexagonal boron nitride (hBN) has attracted tremendous attention over the last few years, thanks to its stable structure and its outstanding properties, such as mechanical strength, thermal conductivity, electrical insulation, and lubricant behavior. This work demonstrates that hBN can also improve the rheological and mechanical properties of elastomer composites when used to partially replace silica. In this work, commercially available pristine hBN (hBN-p) was exfoliated and ball-mill treated in air for different durations (2.5, 5, and 10 h milling). Functionalization occurred with the -NH and -OH groups (hBN-OH). The functional groups were detected using Fourier-Transform Infrared pectroscopy (FT-IR) and were estimated to be up to about 7% through thermogravimetric analysis. The presence of an increased amount of oxygen in hBN-OH was confirmed using Scanning Electron Microscopy coupled with Energy-Dispersive X-ray Spectroscopy. (SEM-EDS). The number of stacked layers, estimated using WAXD analysis, decreased to 8–9 in hBN-OH (10 h milling) from about 130 in hBN-p. High-resolution transmission electron microscopy (HR-TEM) and SEM-EDS revealed the increase in disorder in hBN-OH. hBN-p and hBN-OH were used to partially replace silica by 15% and 30%, respectively, by volume, in elastomer composites based on poly(styrene-co-butadiene) from solution anionic polymerization (S-SBR) and poly(1,4-cis-isoprene) from Hevea Brasiliensis (natural rubber, NR) as the elastomers (volume (mm3) of composites released by the instrument). The use of both hBNs in substitution of 30% of silica led to a lower Payne effect, a higher dynamic rigidity, and an increase in E′ of up to about 15% at 70 °C, with similar/lower hysteresis. Indeed, the composites with hBN-OH revealed a better balance of tan delta (higher at low temperatures and lower at high temperatures) and better ultimate properties. The functional groups reasonably promote the interaction of hBN with silica and with the silica’s coupling agent, sulfur-based silane, and thus promoted the interaction with the elastomer chains. The volume of the composite, measured using a high-pressure capillary viscometer, increased by about 500% and 400% after one week of storage in the presence of hBN-p and hBN-OH. Hence, both hBNs improved the processability and the shelf life of the composites. Composites obtained using hBN-OH had even filler dispersion without the detachments of the filler from the elastomer matrix, as shown through TEM micrographs. These results pave the way for substantial improvements in the important properties of silica-based composites for tire compounds, used to reduce rolling resistance and thus the improve environmental impacts.
Influence of 2 Weeks of Mango Ingestion on Inflammation Resolution after Vigorous Exercise
Authors: Sakaguchi Camila A. , Nieman David C. , Omar Ashraf M. , Strauch Renee C. , Williams James C. , Lila Mary Ann , Zhang Qibin
Published: December 2023

Mangoes have a unique nutrient profile (carotenoids, polyphenols, sugars, and vitamins) that we hypothesized would mitigate post-exercise inflammation. This study examined the effects of mango ingestion on moderating exercise-induced inflammation in a randomized crossover trial with 22 cyclists. In random order with trials separated by a 2-week washout period, the cyclists ingested 330 g mango/day with 0.5 L water or 0.5 L of water alone for 2 weeks, followed by a 2.25 h cycling bout challenge. Blood and urine samples were collected pre- and post-2 weeks of supplementation, with additional blood samples collected immediately post-exercise and 1.5-h, 3-h, and 24 h post-exercise. Urine samples were analyzed for targeted mango-related metabolites. The blood samples were analyzed for 67 oxylipins, which are upstream regulators of inflammation and other physiological processes. After 2 weeks of mango ingestion, three targeted urine mango-related phenolic metabolites were significantly elevated compared to water alone (interaction effects, p ≤ 0.003). Significant post-exercise increases were measured for 49 oxylipins, but various subgroup analyses showed no differences in the pattern of change between trials (all interaction effects, p > 0.150). The 2.25 h cycling bouts induced significant inflammation, but no countermeasure effect was found after 2 weeks of mango ingestion despite the elevation of mango gut-derived phenolic metabolites.
GPER/PKA-Dependent Enhancement of Hormone-Sensitive Lipase Phosphorylation in 3T3-L1 Adipocytes by Piceatannol
Authors: Arisawa Kotoko , Matsuoka Ayumi , Ozawa Natsuki , Ishikawa Tomoko , Ichi Ikuyo , Fujiwara Yoko
Published: December 2023

We previously reported that piceatannol (PIC) had an anti-obesity effect only in ovariectomized (OVX) postmenopausal obesity mice. PIC was found to induce the phosphorylation of hormone-sensitive lipase (pHSL) in OVX mice. To elucidate the mechanism by which PIC activates HSL, we investigated the effect of PIC using 3T3-L1 adipocytes. PIC induced HSL phosphorylation at Ser563 in 3T3-L1 cells, as in vivo experiments showed. pHSL (Ser563) is believed to be activated through the β-adrenergic receptor (β-AR) and protein kinase A (PKA) pathways; however, the addition of a selective inhibitor of β-AR did not inhibit the effect of PIC. The addition of a PKA inhibitor with PIC blocked pHSL (Ser563), suggesting that the effects are mediated by PKA in a different pathway than β-AR. The addition of G15, a selective inhibitor of the G protein-coupled estrogen receptor (GPER), reduced the activation of HSL by PIC. Furthermore, PIC inhibited insulin signaling and did not induce pHSL (Ser565), which represents its inactive form. These results suggest that PIC acts as a phytoestrogen and phosphorylates HSL through a novel pathway that activates GPER and its downstream PKA, which may be one of the inhibitory actions of PIC on fat accumulation in estrogen deficiency.
Anti-Inflammatory Activity of Pequi Oil (Caryocar brasiliense): A Systematic Review
Authors: Silva Vitória R. P. , Pinheiro Andréia C. , Ombredane Alicia S. , Martins Natália Ornelas , Luz Glécia V. S. , Carneiro Marcella L. B. , Joanitti Graziella A.
Published: December 2023

Disorders in the inflammatory process underlie the pathogenesis of numerous diseases. The utilization of natural products as anti-inflammatory agents is a well-established approach in both traditional medicine and scientific research, with studies consistently demonstrating their efficacy in managing inflammatory conditions. Pequi oil, derived from Caryocar brasiliense, is a rich source of bioactive compounds including fatty acids and carotenoids, which exhibit immunomodulatory potential. This systematic review aims to comprehensively summarize the scientific evidence regarding the anti-inflammatory activity of pequi oil. Extensive literature searches were conducted across prominent databases (Scopus, BVS, CINAHL, Cochrane, LILACS, Embase, MEDLINE, ProQuest, PubMed, FSTA, ScienceDirect, and Web of Science). Studies evaluating the immunomodulatory activity of crude pequi oil using in vitro, in vivo models, or clinical trials were included. Out of the 438 articles identified, 10 met the stringent inclusion criteria. These studies collectively elucidate the potential of pequi oil to modulate gene expression, regulate circulating levels of pro- and anti-inflammatory mediators, and mitigate oxidative stress, immune cell migration, and cardinal signs of inflammation. Moreover, negligible to no toxicity of pequi oil was observed across the diverse evaluated models. Notably, variations in the chemical profile of the oil were noted, depending on the extraction methodology and geographical origin. This systematic review strongly supports the utility of pequi oil in controlling the inflammatory process. However, further comparative studies involving oils obtained via different methods and sourced from various regions are warranted to reinforce our understanding of its effectiveness and safety.
Effect of Disinfection Method and Testing Methodology on the Performance of a Breath-Enhanced Jet Nebulizer
Authors: Agoramurthi Kanjanamala , Berlinski Ariel
Published: December 2023

National guidelines for cystic fibrosis recommend cleaning and disinfecting nebulizers after each use. We tested two groups of five reusable breath-enhanced nebulizers after 0, 5, 10, 15, 20, 30, 60, 90, 120, 150, and 180 sterilization (baby bottle sterilizer) or cleaning cycles. The nebulizers were operated for 7 min (6 L/min) after loading albuterol (2.5 mg/3 mL), and they were evaluated with and without breathing simulation after cleaning/sterilization (0–180 and 0–60 cycles, respectively). Over the course of 180 cleaning/sterilization cycles, the mean (SD) solution output was 1.33 mL (0.12 mL)/1.29 mL (0.08 mL); the nebulizer mass remaining in the nebulizer was 61.5% (5.2%)/63% (4%); sputtering time was 4.7 min (0.8 min)/4.8 s (0.6 min); inspiratory filter was 19% (3%)/18.5% (2.4%); expiratory filter was 6.7% (1.1%)/6.7% (0.8%); and difference in drug output calculated using the solution output and nebulizer mass was 6.8% (4%)/5.2% (2.9%). Thermal disinfection with a baby-bottle sterilizer did not alter the performance of a reusable breath-enhanced nebulizer. The nebulizer test performed without breathing simulation underestimated its performance. The calculation of the drug output based on the solution output resulted in its overestimation.
A Parabolic Waveform Generator Based on the Chirp Characteristics of a Directly Modulated Laser
Authors: Chen Na , Jiang Yang , Lan Xiaohong , Zi Yuejiao , Xu Jing , Li Jiahui , Zhang Qiong , Feng Jinjian
Published: December 2023

Due to carrier dynamics, the modulated light field from a directly modulated laser (DML) has an intensity envelope with a certain frequency chirp. When the chirp is linearly mapped into intensity by a frequency discriminator such as an optical filter with a linear edge, the optical field presents a new signal determined by the multiplication operation between the envelope function and the chirp function. Under a triangular drive signal, this process can contribute dark, bright and frequency-doubled bright parabolic waveforms by properly adjusting the filter window. This method is verified by both a theoretical analysis and experimental demonstrations. It not only provides a low-cost and simple scheme to generate parabola signals, but also a new method for arbitrary waveform generation.
Towards Implementation of 3D Amplitude Shaping at 515 nm and First Pulseshaping Experiments at PITZ
Authors: Hoffmann Andreas , Good James , Gross Matthias , Krasilnikov Mikhail , Stephan Frank
Published: December 2023

A key issue of X-ray free-electron lasers is the quality of the photoelectron beams generated from a photocathode by laser pulses in the high-gradient RF gun. Controlling the shape of these laser pulses can strongly reduce the transverse emittance of the generated electron bunch. For this purpose, a laser pulseshaper at 515 nm is presented that can be used directly with alkali antimonide photocathodes for photoemission. The first results regarding generation and measurement of flattop and parabolic pulses as well as introduction of modulations for THz generation are presented and show the potential for emittance optimization with 3D ellipsoidal pulses with the proposed pulseshaper. The experiments are carried out for Cs2Te photocathodes, which require second harmonic generation of the shaped pulses and thus allow investigation of pulseshape preservation in nonlinear frequency conversion processes.