bluuebunny/crossref_metadata_2025 huggingface dataset:
Experimental investigation of enhancement heat dissipation in a novel heat sink under simultaneous impact of impingement jet and magnetic field
Authors: Azadi Shervin , Abjadi Ali , Vahdat Azad Abazar , Ahmadi Danesh Ashtiani Hossein , Afshar Hossein
Published: August 2024

In today’s world, research on cooling systems that can effectively reduce the cooling time of heat sinks and enhance heat transfer efficiency in a shorter duration is extremely crucial. This experimental study analyzed the cooling time by simultaneously utilizing two cooling techniques. This involved the passage of ferrofluid (Fe3O4/water) through three channels of a heat sink under the influence of a magnetic field, as well as the application of an air impingement jet on the heat sink’s surface. A novel plate-fin heat sink with dimensions of 40 mm (L) × 31.32 mm (W) × 23.5 mm (H) consists of three channels with a diameter of 3 mm for crossing ferrofluid flow and 24 channels on the top of the heat sink for crossing the air was designed and built for the study. The heat sink was exposed to an air impingement jet and an external magnetic field simultaneously. Multiple tests were conducted to determine the convective heat transfer coefficients of the heat sink over time under specific conditions, including a magnetic field of 800 G, a volume fraction of 3%, a Reynolds number of 600 for the ferrofluid, and a mass flow rate of 0.1 kg/s. It was discovered that using the combined method of magnetic field and impingement jet (MF-IJ) compared to cooling by air impingement jet with the simultaneous passage of pure water through the ferrofluid channels (Water-IJ), as well as the ferrofluid flow under a magnetic field without the impingement jet (MF) methods for the designed heat sink, can increase the maximum heat transfer coefficient by 27.4% and 46.4%, respectively. The findings of this study suggest that using the MF-IJ cooling method as a flow control mechanism, along with this innovative geometry, can reduce the cooling time of the heat sinks.
Identification and validation of novel engineered AAV capsid variants targeting human glia
Authors: Giacomoni Jessica , Åkerblom Malin , Habekost Mette , Fiorenzano Alessandro , Kajtez Janko , Davidsson Marcus , Parmar Malin , Björklund Tomas
Published: August 2024

Direct neural conversion of endogenous non-neuronal cells, such as resident glia, into therapeutic neurons has emerged as a promising strategy for brain repair, aiming to restore lost or damaged neurons. Proof-of-concept has been obtained from animal studies, yet these models do not efficiently recapitulate the complexity of the human brain, and further refinement is necessary before clinical translation becomes viable. One important aspect is the need to achieve efficient and precise targeting of human glial cells using non-integrating viral vectors that exhibit a high degree of cell type specificity. While various naturally occurring or engineered adeno-associated virus (AAV) serotypes have been utilized to transduce glia, efficient targeting of human glial cell types remains an unsolved challenge. In this study, we employ AAV capsid library engineering to find AAV capsids that selectively target human glia in vitro and in vivo. We have identified two families of AAV capsids that induce efficient targeting of human glia both in glial spheroids and after glial progenitor cell transplantation into the rat forebrain. Furthermore, we show the robustness of this targeting by transferring the capsid peptide from the parent AAV2 serotype onto the AAV9 serotype, which facilitates future scalability for the larger human brain.
The influence of competitive anxiety of Chinese elite swimmers
Authors: Zhou Yuhang , Jin Zhenyu , Wen Yuhong
Published: August 2024

Background

Competitive anxiety is a common stress response in competitive environments, influencing sports performance, particularly among elite swimmers.

Purpose

This study aims to examine how the characteristics of competitive anxiety impact sports performance across different phases of competitive preparation, alongside its correlation with trait anxiety.

Methods

A longitudinal research design, integrating quantitative and qualitative approaches, was employed with 20 swimmers from the Chinese national swimming team participating in both the 2023 Fukuoka World Championships and the Hangzhou Asian Games. The mental readiness form-3 (MRF-3) scale was employed at four time points during the preparation period for longitudinal tracking, complemented by the trait anxiety inventory (T-AI) scale to assess trait anxiety.

Results

The study identified a negative correlation between cognitive anxiety and somatic anxiety among elite swimmers, with confidence demonstrating a positive correlation. Additionally, there was a significant between trait anxiety and competitive anxiety (p < 0.05). Variations in competitive anxiety were found at different stages of the preparation cycle (F = 15.074; 11.627; 19.552, p < 0.05), impacting sport performance.

Conclusion

To optimise performance, tailored psychological intervention programs should be developed and implemented to address the distinct characteristics of competitive anxiety experienced by elite swimmers throughout the preparation phases.
Metabolic profiles associated with exposure to ambient particulate air pollution: findings from the Betula cohort
Authors: Raza Wasif , Öhman Anders , Kanninen Katja M. , Jalava Pasi , Zeng Xiao-wen , de Crom Tosca O. E. , Ikram M. Arfan , Oudin Anna
Published: August 2024

Introduction

Air pollution is a significant contributor to morbidity and mortality globally and has been linked to an increased risk of dementia. Previous studies within the Betula cohort in Northern Sweden have demonstrated associations between air pollution and dementia, as well as distinctive metabolomic profiles in dementia patients compared to controls. This study aimed to investigate whether air pollution is associated with quantitative changes in metabolite levels within this cohort, and whether future dementia status would modify this association.

Methods

Both short-term and long-term exposure to air pollution were evaluated using high spatial resolution models and measured data. Air pollution from vehicle exhaust and woodsmoke were analyzed separately. Metabolomic profiling was conducted on 321 participants, including 58 serum samples from dementia patients and a control group matched for age, sex, and education level, using nuclear magnetic resonance spectroscopy.

Results

No statistically significant associations were found between any metabolites and any measures of short-term or long-term exposure to air pollution. However, there were trends potentially suggesting associations between both long-term and short-term exposure to air pollution with lactate and glucose metabolites. Notably, these associations were observed despite the lack of correlation between long-term and short-term air pollution exposure in this cohort. There were also tendencies for associations between air pollution from woodsmoke to be more pronounced in participants that would later develop dementia, suggesting a potential effect depending on urban/rural factors.

Discussion

While no significant associations were found, the trends observed in the data suggest potential links between air pollution exposure and changes in lactate and glucose metabolites. These findings provide some new insights into the link between air pollution and metabolic markers in a low-exposure setting. However, addressing existing limitations is crucial to improve the robustness and applicability of future research in this area. The pronounced associations in participants who later developed dementia may indicate an influence of urban/rural factors, warranting further investigation.
Prevalence of canine distemper in minks, foxes and raccoon dogs from 1983 to 2023 in Asia, North America, South America and Europe
Authors: Liang Jian , Wang Tingting , Wang Qi , Wang Xiaolin , Fan Xinying , Hu Tingting , Leng Xue , Shi Kun , Li Jianming , Gong Qinglong , Du Rui
Published: August 2024

Canine distemper (CD) is a virulent disease caused by the canine distemper virus (CDV) in canines and mustelidaes with high mortality. The incidence of CDV is worldwide distribution and it has caused huge economic losses to multiple industries around the world. There are many studies investigating the prevalence of CD infection, but no comprehensive analysis of CDV infection in minks, foxes and raccoon dogs worldwide has therefore been carried out. The aim of this meta is to provide a comprehensive assessment of the prevalence of CDV infection in minks, foxes and raccoon dogs dogs through a meta-analysis of articles published from around the world. Data from 8,582 small carnivores in 12 countries were used to calculate the combined prevalence of CD. A total of 22.6% (1,937/8,582) of minks, foxes and raccoon dogs tested positive for CD. The prevalence was higher in Asia (13.8, 95% CI: 22.2–45.6), especially in South Korea (65.8, 95% CI: 83.3–95.8). Our study found that the incidence of CD was also associated with geographic climate, population size, health status, and breeding patterns. CD is more commonly transmitted in minks, foxes and raccoon dogs. However, the concentrated breeding as an economic animal has led to an increase in the prevalence rate. The difference analysis study recommended that countries develop appropriate preventive and control measures based on the prevalence in the minks, foxes, and raccoon dogs industries, and that reducing stocking density is important to reduce the incidence of CDV. In addition, CDV is more common in winter, so vaccination in winter should be strengthened and expanded to reduce the incidence of CD in minks, foxes and raccoon dogs.
Phylogenetic-based methods for fine-scale classification of PRRSV-2 ORF5 sequences: a comparison of their robustness and reproducibility
Authors: VanderWaal Kimberly , Pamornchainavakul Nakarin , Kikuti Mariana , Linhares Daniel C. L. , Trevisan Giovani , Zhang Jianqiang , Anderson Tavis K. , Zeller Michael , Rossow Stephanie , Holtkamp Derald J. , Makau Dennis N. , Corzo Cesar A. , Paploski Igor A. D.
Published: August 2024

Disease management and epidemiological investigations of porcine reproductive and respiratory syndrome virus-type 2 (PRRSV-2) often rely on grouping together highly related sequences. In the USA, the last five years have seen a major shift within the swine industry when classifying PRRSV-2, beginning to move away from RFLP (restriction fragment length polymorphisms)-typing and adopting the use of phylogenetic lineage-based classification. However, lineages and sub-lineages are large and genetically diverse, making them insufficient for identifying new and emerging variants. Thus, within the lineage system, a dynamic fine-scale classification scheme is needed to provide better resolution on the relatedness of PRRSV-2 viruses to inform disease management and monitoring efforts and facilitate research and communication surrounding circulating PRRSV viruses. Here, we compare fine-scale systems for classifying PRRSV-2 variants (i.e., genetic clusters of closely related ORF5 sequences at finer scales than sub-lineage) using a database of 28,730 sequences from 2010 to 2021, representing >55% of the U.S. pig population. In total, we compared 140 approaches that differed in their tree-building method, criteria, and thresholds for defining variants within phylogenetic trees. Three approaches resulted in variant classifications that were reproducible and robust even when the input data or input phylogenies were changed. For these approaches, the average genetic distance among sequences belonging to the same variant was 2.1–2.5%, and the genetic divergence between variants was 2.5–2.7%. Machine learning classification algorithms were trained to assign new sequences to an existing variant with >95% accuracy, which shows that newly generated sequences can be assigned to a variant without repeating the phylogenetic and clustering analyses. Finally, we identified 73 sequence-clusters (dated <1 year apart with close phylogenetic relatedness) associated with circulation events on single farms. The percent of farm sequence-clusters with an ID change was 6.5–8.7% for our approaches. In contrast, ~43% of farm sequence-clusters had variation in their RFLP-type, further demonstrating how our proposed fine-scale classification system addresses shortcomings of RFLP-typing. Through identifying robust and reproducible classification approaches for PRRSV-2, this work lays the foundation for a fine-scale system that would more reliably group related field viruses and provide better resolution for decision-making surrounding disease management.
Preparation, solubility, and anti-inflammatory effects of a complex of diphenylcyclopropenone/β-cyclodextrin derivatives as the treatment of alopecia areata
Authors: Inoue Yutaka , Yoshino Kaede , Kudo Suzu , Kodama Nao , Moteki Hajime , Kimura Mitsutoshi
Published: August 2024

Purpose

To investigate the preparation of inclusion complexes of diphenylcyclopropenone (DPCP)/β-cyclodextrin (β-CD) derivatives using a three-dimensional (3D) ball mill, and verify the inclusion behavior of the solid dispersion. Additionally, we aimed to investigate the effect of DPCP/β-CDs complex formation on the spleens of male C57BL/6 mice in terms of anti-inflammatory effects.

Methods

The inclusion complexes of DPCP with β-CD and hydroxypropyl-β-cyclodextrin (HPβCD) were prepared using a 3D ball mill. Powder X-ray diffraction (PXRD) and Fourier-transform infrared spectroscopy (FT-IR) were used to evaluate the solid-state properties. The solubility of the prepared DPCP/β-CD and HPβCD complexes and the intermolecular interaction between DPCP and β-CD derivatives in solution were assessed using 1H nuclear magnetic resonance (NMR). Furthermore, the anti-inflammatory effects of DPCPs in the prepared DPCP/CD complexes were investigated using spleens from male C57BL/6 mice, with measurement of interferon gamma (IFN-γ) secretion as an endpoint. Additionally, the protective effects of each drug on NIH-3T3 cells exposed to ultraviolet (UV) irradiation were examined.

Results

Solid-state characterization confirmed the formation of inclusion complexes in the 3D ground mixture (3DGM) (DPCP/β-CD = 1/1) and 3DGM (DPCP/HPβCD = 1/1) complexes through PXRD and IR analysis. The solubility of 3DGM (DPCP/β-CD = 1/1) and 3DGM (DPCP/HPβCD = 1/1) was 17.5 μg/mL and 58.4 μg/mL, respectively, indicating higher solubility than that of DPCP alone. NMR analysis of 3DGM samples suggested that DPCP/β-CD and DPCP/HPβCD form inclusion complexes at a molar ratio of 1/1 but with different inclusion modes. Regarding the anti-inflammatory activity of DPCP, 3DGM (DPCP/HPβ-CD) showed anti-inflammatory effects at lower doses compared to 3DGM (DPCP/β-CD) in terms of IFN-γ and NIH-3T3 cells injured by UV irradiation.

Conclusion

We successfully formed inclusion complexes of DPCP/β-CD and DPCP/HPβCD using the 3D ground mixture method. NMR analysis suggested that DPCP/β-CD and DPCP/HPβCD form inclusion complexes at a molar ratio of 1/1 but with different inclusion modes. The anti-inflammatory activity of DPCP was more pronounced in 3DGM (DPCP/HPβCD) at lower doses compared to that in 3DGM (DPCP/β-CD), indicating that the HPβCD derivatives were more effective in enhancing the anti-inflammatory properties of DPCP.
Propeller Effects and Elasticity in Aerodynamic Analysis of Small Propeller-Driven Aircraft and UAVs
Authors: Rostami Mohsen
Published: August 2024

The importance of propeller effects and power contribution to the aerodynamics of small aircraft and unmanned aerial vehicles (UAVs) is indispensable. The aerodynamic analysis of wings in flight varies from rigid wing analysis due to wing deflection caused by transferred aerodynamic loads. This paper investigates the intertwined influence of propeller effects and elasticity on the aerodynamics of small propeller-driven aircraft and UAVs. Through a detailed methodology, a twin-engine propeller-driven aircraft is analyzed as a case study, providing insights into the proposed approach. Two critical analyses are presented: an examination of propeller effects in rigid aircraft and the incorporation of elastic wing properties. The former establishes a foundational understanding of aerodynamic behavior, while the latter explores the impact of wing elasticity on performance. Validation is achieved through comparative analysis with wind tunnel test results from a similar rigid structure aircraft. Utilizing NASTRAN software V2010.1, aerodynamic analysis of the elastic aircraft is conducted, complemented by semi-empirical insights. The results highlight the importance of these factors across different angles of attack. Furthermore, deviations from the rigid aircraft configuration emphasize the considerable influence of static aeroelasticity analysis, notably increasing longitudinal characteristics by approximately 20%, while showing a lower impact of 5% in lateral-directional characteristics. This study contributes to enhanced design and operational considerations for small propeller-driven aircraft, with implications for future research and innovation, particularly for the purpose of efficient concepts in advanced air mobility.
MicroRNAomic Analysis of Spent Media from Slow- and Fast-Growing Bovine Embryos Reveal Distinct Differences
Authors: Rio Paul Del , DiMarco Sierra , Madan Pavneesh
Published: August 2024

In bovine embryos, the microRNA (miRNA) expression has been profiled at each stage of early development in vitro. The miRNAomic analysis of spent media has the potential to reveal characteristics of embryo health; however, applications are limited without categorizing miRNA profiles by embryo quality. Time-lapse imaging has shown the timing of embryo development in vitro may be indicative of their developmental potential. The study aimed to characterize miRNAs in the spent media of bovine embryos with different growth rates during the pre-implantation phase. Bovine cumulus–oocyte complexes were aspirated from ovaries, fertilized, and cultured to blastocyst stage of development. At the 2-cell, 8-cell, and blastocyst stage, each microdrop of 30 presumptive zygotes were classified as slow- or fast-growing based on the percentage of embryos that had reached the desired morphological stage. A comparative analysis was performed on the spent media of slow- and fast-growing embryos using the results of a GeneChip miRNA 4.0 array hybridization. In total, 34 differentially expressed miRNAs were identified between the comparison groups: 14 miRNAs were found in the 2-cell samples, 7 in the 8-cell samples, and 12 in the blastocyst samples. The results demonstrate distinct miRNAs populations can be identified between slow- and fast-growing embryos, highlighting the novel biomarkers of developmental potential at each stage of pre-implantation development.
Structural Analysis of the Historical Sungurlu Clock Tower
Authors: Gökdemir Ahmet , Baki Zülküf
Published: August 2024

Background: The strength of historical buildings built in different centuries with various materials and construction techniques and harboring many structural problems depends on the structural system, geometrical condition, and material properties. Sungurlu clock tower, whose system and geometry are in good condition, has been damaged under environmental and climatic effects, earthquakes, and other loads, and has survived to the present day by preserving its structural integrity to a great extent with the repairs it has undergone. Methods: In addition to static analysis, the robustness and durability of the design of the tower were tested by dynamic analysis with the SAP2000 program. In the model that will represent the actual system behavior of the tower, the lengths of the elements; nodal points; bearings; joints; shapes such as bars, shells, and plates; characteristic values of the materials to be used; as well as the system, element sections, and all loads and combinations of masses or dynamic forces acting on the system are defined. Results: In the reports presented visually, the moment, shear force, axial forces, and other forces to which the tower was exposed after the architectural and structural problems were eliminated were seen in a diagram. Since the effects of the damage could not be predicted, in this study, to measure the reaction of the building against earthquakes and other loads, the numerical model representing its original condition was prepared and analyzed according to the theoretical method and assumptions made by the restitution, survey, and static observation reports. Conclusions: With this program, which allows for the preparation of this model, it was concluded that the loads coming to the structure according to the principles of ductility, rigidity, and strength could be safely transferred to the ground without causing damage to the structural system and its elements. From the deformation, stress, velocity, acceleration, and reaction force graphs obtained, it was understood that the tower exhibited the expected structural behavior under its own weight and live loads. The stress and reaction force graphs showed that the structural materials are adequate for the resistance of the structure and system against the existing loads and possible earthquakes.
Generalization of Fixed-Point Results in Complex-Valued Bipolar Metric Space with Applications
Authors: Shammaky Amnah Essa , Ahmad Jamshaid
Published: August 2024

We undertake this study with the objective of introducing certain control functions in the contractive condition to prove fixed-point theorems in the framework of complex-valued bipolar metric spaces. The incorporation of control functions broadens the applicability of the contractive condition. This approach yields key results consistent with previous studies. In support of our results, we offer two insightful examples that demonstrate the concepts discussed. Additionally, we present the notion of interpolative contraction in this new and generalized metric space and prove fixed-point theorems for non-self mappings. To demonstrate the application of our approach, we reproduce key findings from several established studies in the field.
Extraintestinal Manifestations in Inflammatory Bowel Disease: From Pathophysiology to Treatment
Authors: Faggiani Ilaria , Fanizza Jacopo , D’Amico Ferdinando , Allocca Mariangela , Zilli Alessandra , Parigi Tommaso Lorenzo , Barchi Alberto , Danese Silvio , Furfaro Federica
Published: August 2024

The inflammatory bowel diseases (IBDs) are systemic conditions that affect not only the gastrointestinal tract but also other parts of the body. The presence of extraintestinal manifestations can significantly impact the quality of life in IBD patients. Peripheral arthritis, episcleritis, and erythema nodosum are frequently associated with active intestinal inflammation and often improve with standard treatment targeting intestinal inflammation. In contrast, anterior uveitis, ankylosing spondylitis, and primary sclerosing cholangitis typically occur independently of disease flares. The incidence of these conditions in individuals with IBD can reach up to 50% of patients over the course of their lifetime. In addition, some advanced therapies utilized for the treatment of IBD potentially result in side effects that may resemble extraintestinal manifestations. This review provides a thorough analysis of the pathophysiology and treatment of extraintestinal manifestations associated with Crohn’s disease and ulcerative colitis.
Bioinformatics Identification and Experimental Verification of Disulfidptosis-Related Genes in the Progression of Osteoarthritis
Authors: Cao Siyang , Wei Yihao , Yue Yaohang , Wang Deli , Xiong Ao , Yang Jun , Zeng Hui
Published: August 2024

Background: Osteoarthritis (OA) is a disabling and highly prevalent condition affecting millions worldwide. Recently discovered, disulfidptosis represents a novel form of cell death induced by the excessive accumulation of cystine. Despite its significance, a systematic exploration of disulfidptosis-related genes (DRGs) in OA is lacking. Methods: This study utilized three OA-related datasets and DRGs. Differentially expressed (DE)-DRGs were derived by intersecting the differentially expressed genes (DEGs) from GSE114007 with DRGs. Feature genes underwent screening through three machine learning algorithms. High diagnostic value genes were identified using the receiver operating characteristic curve. Hub genes were confirmed through expression validation. These hub genes were then employed to construct a nomogram and conduct enrichment, immune, and correlation analyses. An additional validation of hub genes was performed through in vitro cell experiments. Results: SLC3A2 and PDLIM1 were designated as hub genes, displaying excellent diagnostic performance. PDLIM1 exhibited low expression in early chondrocyte differentiation, rising significantly in the late stage, while SLC3A2 showed high overall expression, declining in the late differentiation stage. Cellular experiments corroborated the correlation of SLC3A2 and PDLIM1 with chondrocyte inflammation. Conclusions: Two hub genes, SLC3A2 and PDLIM1, were identified in relation to disulfidptosis, providing potential directions for diagnosing and treating OA.
Upholding or Breaking the Law of Superposition in Pharmacokinetics
Authors: Yousef Malaz , Yáñez Jaime A. , Löbenberg Raimar , Davies Neal M.
Published: August 2024

The law of superposition underpins first-order linear pharmacokinetic relationships. Most drugs, therefore, after a single dose can be described by first-order or linear processes, which can be superposed to understand multiple-dose regimen behavior. However, there are a number of situations where drugs could display behaviors after multiple dosing that leads to capacity-limited or saturation non-linear kinetics and the law of superposition is overruled. This review presents a practical guide to understand the equations and calculations for single and multiple-dosing regimens after intravenous and oral administration. It also provides the pharmaceutical basis for saturation in ADME processes and the consequent changes in the area under the concentration–time curve, which represents drug exposure that can lead to the modulation of efficacy and/or toxic effects. The pharmacokineticist must implicitly understand the principles of superposition, which are a central tenet of drug behavior and disposition during drug development.
Impact of the Anticholinergic Burden on Disease-Specific Symptoms in Parkinsonian Syndromes
Authors: Mahmoudi Romina , Greten Stephan , Veith Sanches Linda , Krey Lea , Ulaganathan Sarana , Höglinger Günter U. , Heck Johannes , Wegner Florian , Klietz Martin
Published: August 2024

Background: Anticholinergic adverse effects pose a relevant threat to patients, in particular elderly and cognitively impaired patients. Patients with Parkinsonian syndromes are especially at risk from anticholinergic adverse effects due to the often-required complex drug therapy. Aims: The aim of this study was to evaluate the potential effect of the anticholinergic burden on motor and non-motor symptoms in Parkinson’s disease and atypical Parkinsonian syndromes. Methods: This cross-sectional, monocentric retrospective data analysis included 151 patients with Parkinson’s disease (PD), 63 with progressive supranuclear palsy (PSP), and 36 with multiple system atrophy (MSA). The anticholinergic burden of patients’ medications was determined using two established scores: the Anticholinergic Drug Scale (ADS) and the German Anticholinergic Burden Scale (GABS). These scores were compared between the different diseases and correlated with several disease-specific scores. Results: Anticholinergic burden was higher in patients with PD, in particular, compared to PSP. In the PD group, anticholinergic burden showed a weak correlation with almost all analyzed clinical scores and the number of administered drugs. The UMSARS I and II showed a significant correlation with the anticholinergic burden in MSA patients. In general, the GABS-measured anticholinergic burden was significantly higher compared to the ADS-measured. Conclusions: The calculated anticholinergic burden affected motor and non-motor symptoms in patients with various Parkinsonian syndromes poorly. Since the GABS also contains basic anti-parkinsonian drugs, this score tended to overestimate the anticholinergic burden in patients with Parkinsonian syndromes and, therefore, seemed less appropriate for this application.
Antispasmodic Agents in Magnetic Resonance Imaging of the Urinary Bladder—A Narrative Review
Authors: Sklinda Katarzyna , Rajca Martyna , Mruk Bartosz , Walecki Jerzy
Published: August 2024

Accurate assessment of muscular layer infiltration of the urinary bladder wall is crucial for diagnostic precision and is significantly influenced, among other factors, by the elimination of motion artifacts. This review explores the potential benefits of using spasmolytic agents to achieve improved imaging results. Specifically, it examines two commonly available pharmaceutical preparations: butylscopolamine (buscolysin) and glucagon. The review highlights the similarities and differences between these agents and discusses the optimal methods of administration to enhance urinary bladder imaging. By addressing these factors, the article aims to provide insights into improving diagnostic accuracy in clinical practice.
Value of Spinal Cord Diffusion Imaging and Tractography in Providing Predictive Factors for Tumor Resection in Patients with Intramedullary Tumors: A Pilot Study
Authors: Dauleac Corentin , Jacquesson Timothée , Frindel Carole , André-Obadia Nathalie , Ducray François , Mertens Patrick , Cotton François
Published: August 2024

This pilot study aimed to investigate the interest of high angular resolution diffusion imaging (HARDI) and tractography of the spinal cord (SC) in the management of patients with intramedullary tumors by providing predictive elements for tumor resection. Eight patients were included in a prospective study. HARDI images of the SC were acquired using a 3T MRI scanner with a reduced field of view. Opposed phase-encoding directions allowed distortion corrections. SC fiber tracking was performed using a deterministic approach, with extraction of tensor metrics. Then, regions of interest were drawn to track the spinal pathways of interest. HARDI and tractography added value by providing characteristics about the microstructural organization of the spinal white fibers. In patients with SC tumors, tensor metrics demonstrated significant changes in microstructural architecture, axonal density, and myelinated fibers (all, p < 0.0001) of the spinal white matter. Tractography aided in the differentiation of tumor histological types (SC-invaded vs. pushed back by the tumor), and differentiation of the spinal tracts enabled the determination of precise anatomical relationships between the tumor and the SC, defining the tumor resectability. This study underlines the value of using HARDI and tractography in patients with intramedullary tumors, to show alterations in SC microarchitecture and to differentiate spinal tracts to establish predictive factors for tumor resectability.
Potentiation by Protein Synthesis Inducers of Translational Readthrough of Pathogenic Premature Termination Codons in PTEN Isoforms
Authors: Torices Leire , Nunes-Xavier Caroline E. , Pulido Rafael
Published: August 2024

The PTEN tumor suppressor is frequently targeted in tumors and patients with PTEN hamartoma tumor syndrome (PHTS) through nonsense mutations generating premature termination codons (PTC) that may cause the translation of truncated non-functional PTEN proteins. We have previously described a global analysis of the readthrough reconstitution of the protein translation and function of the human canonical PTEN isoform by aminoglycosides. Here, we report the efficient functional readthrough reconstitution of the PTEN translational isoform PTEN-L, which displays a minimal number of PTC in its specific N-terminal extension in association with disease. We illustrate the importance of the specific PTC and its nucleotide proximal sequence for optimal readthrough and show that the more frequent human PTEN PTC variants and their mouse PTEN PTC equivalents display similar patterns of readthrough efficiency. The heterogeneous readthrough response of the different PTEN PTC variants was independent of the length of the PTEN protein being reconstituted, and we found a correlation between the amount of PTEN protein being synthesized and the PTEN readthrough efficiency. Furthermore, combination of aminoglycosides and protein synthesis inducers increased the readthrough response of specific PTEN PTC. Our results provide insights with which to improve the functional reconstitution of human-disease-related PTC pathogenic variants from PTEN isoforms by increasing protein synthesis coupled to translational readthrough.
The Case for Bisphosphonate Use in Astronauts Flying Long-Duration Missions
Authors: Rosenthal Reece , Schneider Victor S. , Jones Jeffrey A. , Sibonga Jean D.
Published: August 2024

Changes in the structure of bone can occur in space as an adaptive response to microgravity and on Earth due to the adaptive effects to exercise, to the aging of bone cells, or to prolonged disuse. Knowledge of cell-mediated bone remodeling on Earth informs our understanding of bone tissue changes in space and whether these skeletal changes might increase the risk for fractures or premature osteoporosis in astronauts. Comparisons of skeletal health between astronauts and aging humans, however, may be both informative and misleading. Astronauts are screened for a high level of physical fitness and health, are launched with high bone mineral densities, and perform exercise daily in space to combat skeletal atrophy as an adaptive response to reduced weight-bearing function, while the elderly display cellular and tissue pathology as a response to senescence and disuse. Current clinical testing for age-related bone change, applied to astronauts, may not be sufficient for fully understanding risks associated with rare and uniquely induced bone changes. This review aims to (i) highlight cellular analogies between spaceflight-induced and age-related bone loss, which could aid in predicting fractures, (ii) discuss why overreliance on terrestrial clinical approaches may miss potentially irreversible disruptions in trabecular bone microarchitecture induced by spaceflight, and (iii) detail how the cellular effects of the bisphosphonate class of drugs offer a prophylactic countermeasure for suppressing the elevated bone resorption characteristically observed during long-duration spaceflights. Thus the use of the bisphosphonate will help protect the bone from structural changes while in microgravity either along with exercise or alone when exercise is not performed, e.g. after an injury or illness.
Extraction of Anthocyanin Dye from Staghorn Sumac Fruit in Various Solvents and Use for Pigment Printing
Authors: Klančnik Maja , Koradin Elena
Published: August 2024

This study investigates the potential of the dye extracted from the fruits of the alien invasive plant staghorn sumac (lat. Rhus typhina) as a sustainable and environmentally friendly colorant. By using a range of solvents, including distilled water, methanol, ethanol, propanol, acetonitrile, acetone, and dichloromethane, this study aims to determine the optimum solvent for the extraction of anthocyanin dyes from the fruit of staghorn sumac for the formulation of printing inks and for screen printing on paper and cotton fabric. The colors of the prints made with different dye extracts varied between more or less intense brownish-yellow hues, with the exception of the dye extracts in methanol and ethanol, which gave more brownish-orange hues. All prints showed excellent resistance to rubbing on cotton fabrics as well as to wet ironing. The light fastness of prints made with inks containing dyes extracted from all organic solvents was very good. Good wash fastness of prints on cotton fabrics was only achieved with inks made with dyes extracted in propanol and dichloromethane. The ink made from the dye extracted in propanol proved to be the best choice for printing on cotton fabric due to its uniform, intense, and resilient prints, while the inks made from the dyes extracted in distilled water and ethanol were also a good choice for printing on paper.
Ni-MoO2 Composite Coatings Electrodeposited at Porous Ni Substrate as Efficient Alkaline Water Splitting Cathodes
Authors: Petričević Aleksandar , Gojgić Jelena , Bernäcker Christian I. , Rauscher Thomas , Bele Marjan , Smiljanić Milutin , Hodnik Nejc , Elezović Nevenka , Jović Vladimir D. , Krstajić Pajić Mila N.
Published: August 2024

To obtain highly efficient yet easily produced water-splitting cathodes, Ni-MoO2 composite coatings were electrodeposited at a Ni foam substrate with an open-pore structure, pore size of 450 µm, in a Watts-type bath. The concentration of MoO2 particles (about 100 nm) was varied, while the intensive mixing of the solution was provided by air bubbling with 0.5 L min−1. Electrodeposition was performed at different constant current densities at room temperature. The morphology and composition of the coatings were investigated by SEM and EDS. The hydrogen evolution reaction (HER) was tested in KOH of different concentrations, at several temperatures, in a three-electrode H-cell by recording polarization curves and EIS measurements. The lowest achieved HER overpotential was −158 mV at −0.5 A cm−2. Up-scaled samples, 3 × 3.3 cm2, were tested in a single zero-gap cell showing decreasing cell voltage (from 2.18 V to 2.11 V) at 0.5 A cm−2 over 5 h in 30% KOH at 70 °C with electrolyte flow rate of 58 mL min−1. Compared to pure Ni foams used as both cathode and anode under the same conditions, the cell voltage is decreased by 200 mV, showing improved electrode performance.
Extended Caking Method for Strain Analysis of Polycrystalline Diffraction Debye–Scherrer Rings
Authors: Uzun Fatih , Daisenberger Dominik , Liogas Konstantinos , Wang Zifan Ivan , Chen Jingwei , Besnard Cyril , Korsunsky Alexander M.
Published: August 2024

Polycrystalline diffraction is a robust methodology employed to assess elastic strain within crystalline components. The Extended Caking (exCaking) method represents a progression of this methodology beyond the conventional azimuthal segmentation (Caking) method for the quantification of elastic strains using Debye–Scherrer 2D X-ray diffraction rings. The proposed method is based on the premise that each complete diffraction ring contains comprehensive information about the complete elastic strain variation in the plane normal to the incident beam, which allows for the introduction of a novel algorithm that analyses Debye–Scherrer rings with complete angular variation using ellipse geometry, ensuring accuracy even for small eccentricity values and offering greater accuracy overall. The console application of the exCaking method allows for the accurate analysis of polycrystalline X-ray diffraction data according to the up-to-date rules presented in the project repository. This study presents both numerical and empirical examinations and error analysis to substantiate the method’s reliability and accuracy. A specific validation case study is also presented to analyze the distribution of residual elastic strains in terms of force balance in a Ti-6Al-4V titanium alloy bar plastically deformed by four-point bending.
Spatio-Temporal Change and Drivers of the Vegetation Trends in Central Asia
Authors: Li Moyan , Yao Junqiang , Zheng Jianghua
Published: August 2024

The impact of changing climate on vegetation in dryland is a prominent focus of global research. As a typical arid region in the world, Central Asia is an ideal area for studying the associations between climate and arid-area vegetation. Utilizing data from the European Centre for Medium-Range Weather Forecasts fifth-generation reanalysis (ECMWF ERA-5) and normalized difference vegetation index (NDVI) datasets, this study investigates the spatio-temporal variation characteristics of the NDVI in Central Asia. It quantitatively assesses the contribution rates of climatic factors to vegetation changes and elucidates the impact of an increased vapor pressure deficit (VPD) on vegetation changes in Central Asia. The results indicate that the growing seasons’ NDVI exhibited a substantial increase in Central Asia during 1982–2015. Specifically, there was a pronounced “greening” process (0.012/10 yr, p < 0.05) from 1982 to 1998. However, an insignificant “browning” trend was observed after 1998. Spatially, the vegetation NDVI in the growing seasons exhibited a pattern of “greening in the east and browning in the west” of Central Asia. During spring, the dominant theme was the “greening” of vegetation NDVI, although there was noticeable “browning” observed in southwest region of Central Asia. During summer, the “browning” of vegetation NDVI further expanded eastward and impacted the entire western Central Asia in autumn. According to the estimated results computed via the partial differential equation method, the “browning” trend of vegetation NDVI during the growing seasons was guided by increased VPD and decreased rainfall in western Central Asia. Specifically, the increased VPD contributed 52.3% to the observed vegetation NDVI. Atmospheric drought depicted by the increase in VPD significantly lowers the “greening” trend of vegetation NDVI in arid regions, which further aggravates the “browning” trend of vegetation NDVI.
Evaluation of the Morphological and Physicochemical Diversity of Carob (Ceratonia siliqua, Fabaceae) Germplasm from Algeria
Authors: Smaili Oussama , Chebouti-Meziou Nadjiba , Scollo Francesco , La Malfa Stefano , Gentile Alessandra , Distefano Gaetano , Luca Leonardo Paul , Catalano Chiara
Published: August 2024

Despite the economic and ecological importance of the carob tree, few studies are available on the morphological characterization and chemical composition of carob in Algeria. This has resulted in the lack of selection of commercial cultivars of high seed and sugar content. The present study aims to assess the diversity of 11 wild and 1 cultivated carob populations in various regions of Algeria, characterized by diverse geographical and climatic conditions. The final objective is to assess the diversity of neglected carob plants that exhibit superior fruit quality traits and/or are well adapted to different pedoclimatic conditions. The current study focuses on analyzing pod and seed diversity by investigating 12 morphological and 5 physiochemical traits. The analysis of variance revealed significant differences between populations (p < 0.001) for all traits studied, indicating high morphological and chemical variability among these carob populations. Principal component analysis (PCA) was employed to study the relationship between these traits and the geographical origin. High seed yields characterized wild populations, whereas cultivated populations were rich in sugar. Based on morphological traits of pods, seeds and chemical compositions, a hierarchical clustering grouped the carob populations into four groups. The findings of this study represent a significant advancement towards establishing effective cultivar breeding programs in Algeria. They demonstrate that the germplasm examined in this research exhibits an optimal level of morphological and chemical diversity, which is instrumental in the identification of specific traits of both commercial and environmental significance.
Different Nitrogen Consumption Patterns in Low Temperature Fermentations in the Wine Yeast Saccharomyces cerevisiae
Authors: García-Ríos Estéfani , Pardo Judit , Su Ying , Guillamón José Manuel
Published: August 2024

Nowadays, the wine industry carries out fermentations at low temperatures because this oenological practice clearly improves the aromatic complexity of the final wines. In addition, nitrogen content of the must also influences the quality of the wine. In this study, we carried out a phenotypic and fermentative analysis of two industrial wine Saccharomyces cerevisiae strains (P5 and P24) at 15 and 28 °C and three nitrogen concentrations (60, 140 and 300 mg N/L) in synthetic must. Our results show that both parameters, temperature and nitrogen, are interrelated and clearly determine the competitiveness of the wine strains and their ability to adapt at low temperatures. The best adapted strain at low temperatures decreased its competitiveness at lower nitrogen concentrations. In addition, our results show that it is not only the quantity of nitrogen transported that is important but also the quality of the nitrogen source used for wine yeast adaptation at low temperatures. The presence of some amino acids, such as arginine, branched chain amino acids, and some aromatic amino acids can improve the growth and fermentation activity of wine yeasts at low temperatures. These results allow us to better understand the basis of wine yeast adaptation to fermentation conditions, providing important information for winemakers to help them select the most appropriate yeast strain, thus reducing the economic costs associated with long and sluggish fermentations. The correlation between some amino acids and better yeast fermentation performance could be used in the future to design inactive dry yeast enriched in some of these amino acids, which could be added as a nutritional supplement during low temperature fermentations.
Effects of Inoculation with Koji and Strain Exiguobacterium profundum FELA1 on the Taste, Flavor, and Bacterial Community of Rapidly Fermented Shrimp Paste
Authors: Liu Huanming , Huang Ailian , Yi Jiawen , Luo Meiyan , Jiang Guili , Guan Jingjing , Liu Shucheng , Deng Chujin , Luo Donghui
Published: August 2024

This study was conducted to investigate the effect of inoculation with Exiguobacterium profundum FELA1 isolated from traditional shrimp paste and koji on the taste, flavor characteristics, and bacterial community of rapidly fermented shrimp paste. E-nose and e-tongue results showed higher levels of alcohols, aldehydes, and ketones, enhanced umami and richness, and reduced bitterness and astringency in samples of shrimp paste inoculated with fermentation (p < 0.05). Eighty-two volatile compounds were determined using headspace solid-phase microextraction and gas chromatography–mass spectrometry (HS-SPEM-GC-MS). The contents of 3-methyl-1-butanol, phenylethanol, isovaleraldehyde, and 2-nonanone in the inoculated samples were significantly increased (p < 0.05), resulting in pleasant odors such as almond, floral, and fruity. High-throughput sequencing results showed that the addition of koji and FELA1 changed the composition and abundance of bacteria and reduced the abundance of harmful bacteria. Spearman’s correlation coefficient indicated that the alcohols, aldehydes, and ketones of the inoculated fermented samples showed a strong correlation (|ρ| > 0.6) with Virgibacillus and Exiguobacterium, which contributed to the formation of good flavor in the fast fermented shrimp paste. This study may offer new insights into the production of rapidly fermented shrimp paste with better taste and flavor.
Correction: Martel-Rodríguez et al. Long-Term Performance of a Hybrid-Flow Constructed Wetlands System for Urban Wastewater Treatment in Caldera de Tirajana (Santa Lucía, Gran Canaria, Spain). Int. J. Environ. Res. Public Health 2022, 19, 14871
Authors: Martel-Rodríguez Gilberto M. , Millán-Gabet Vanessa , Mendieta-Pino Carlos A. , García-Romero Eva , Sánchez-Ramírez José R.
Published: August 2024

There was an error in the original publication [...]