bluuebunny/crossref_metadata_2025 huggingface dataset:
Dipeptidyl Peptidase (DPP)-4 Inhibitors and Pituitary Adenylate Cyclase-Activating Polypeptide, a DPP-4 Substrate, Extend Neurite Outgrowth of Mouse Dorsal Root Ganglia Neurons: A Promising Approach in Diabetic Polyneuropathy Treatment
Authors: Yamaguchi Masahiro , Noda-Asano Saeko , Inoue Rieko , Himeno Tatsuhito , Motegi Mikio , Hayami Tomohide , Nakai-Shimoda Hiromi , Kono Ayumi , Sasajima Sachiko , Miura-Yura Emiri , Morishita Yoshiaki , Kondo Masaki , Tsunekawa Shin , Kato Yoshiro , Kato Koichi , Naruse Keiko , Nakamura Jiro , Kamiya Hideki
Published: August 2024

Individuals suffering from diabetic polyneuropathy (DPN) experience debilitating symptoms such as pain, paranesthesia, and sensory disturbances, prompting a quest for effective treatments. Dipeptidyl-peptidase (DPP)-4 inhibitors, recognized for their potential in ameliorating DPN, have sparked interest, yet the precise mechanism underlying their neurotrophic impact on the peripheral nerve system (PNS) remains elusive. Our study delves into the neurotrophic effects of DPP-4 inhibitors, including Diprotin A, linagliptin, and sitagliptin, alongside pituitary adenylate cyclase-activating polypeptide (PACAP), Neuropeptide Y (NPY), and Stromal cell-derived factor (SDF)-1a—known DPP-4 substrates with neurotrophic properties. Utilizing primary culture dorsal root ganglia (DRG) neurons, we meticulously evaluated neurite outgrowth in response to these agents. Remarkably, all DPP-4 inhibitors and PACAP demonstrated a significant elongation of neurite length in DRG neurons (PACAP 0.1 μM: 2221 ± 466 μm, control: 1379 ± 420, p < 0.0001), underscoring their potential in nerve regeneration. Conversely, NPY and SDF-1a failed to induce neurite elongation, accentuating the unique neurotrophic properties of DPP-4 inhibition and PACAP. Our findings suggest that the upregulation of PACAP, facilitated by DPP-4 inhibition, plays a pivotal role in promoting neurite elongation within the PNS, presenting a promising avenue for the development of novel DPN therapies with enhanced neurodegenerative capabilities.
Study on the Function of SlWRKY80 in Tomato Defense against Meloidogyne incognita
Authors: Chen Yinxia , Wang Zhize , Nie Weidan , Zhao Tingjie , Dang Yule , Feng Chenghao , Liu Lili , Wang Chaonan , Du Chong
Published: August 2024

WRKY transcription factors (TFs) can participate in plant biological stress responses and play important roles. SlWRKY80 was found to be differentially expressed in the Mi-1- and Mi-3-resistant tomato lines by RNA-seq and may serve as a key node for disease resistance regulation. This study used RNAi to determine whether SlWRKY80 silencing could influence the sensitivity of ‘M82’ (mi-1/mi-1)-susceptible lines to M. incognita. Further overexpression of this gene revealed a significant increase in tomato disease resistance, ranging from highly susceptible to susceptible, combined with the identification of growth (plant height, stem diameter, and leaf area) and physiological (soluble sugars and proteins; root activity) indicators, clarifying the role of SlWRKY80 as a positive regulatory factor in tomato defense against M. incognita. Based on this phenomenon, a preliminary exploration of its metabolic signals revealed that SlWRKY80 stimulates different degrees of signaling, such as salicylic acid (SA), jasmonic acid (JA), and ethylene (ETH), and may synergistically regulate reactive oxygen species (ROS) accumulation and scavenging enzyme activity, hindering the formation of feeding sites and ultimately leading to the reduction of root gall growth. To our knowledge, SlWRKY80 has an extremely high utilization value for improving tomato resistance to root-knot nematodes and breeding.
A One-Month Advanced Glycation End Products—Restricted Diet Improves CML, RAGE, Metabolic and Inflammatory Profile in Patients with End-Stage Renal Disease Undergoing Haemodialysis
Authors: Aroni Adamantia , Detopoulou Paraskevi , Presvelos Demetrios , Kostopoulou Eirini , Ioannidis Anastasios , Panoutsopoulos George I. , Zyga Sofia , Kosmidis Georgios , Spiliotis Bessie E. , Rojas Gil Andrea Paola
Published: August 2024

Exogenous and endogenous advanced glycation end products (AGEs) contribute to the pathogenesis and progression of renal disease. This is a one-month controlled dietary counseling trial that restricts nutritional AGEs in patients with end-stage renal disease (ESRD) undergoing haemodialysis (n = 22 participants in the intervention and n = 20 participants in the control group). Haematological, biochemical markers, the soluble form of the receptor for AGEs (sRAGE), and carboxymethyl lysine (CML) were measured at baseline and at follow-up. Mononuclear cells were isolated and the protein expression of RAGE and the inflammatory marker COX-2 was measured using Western immunoblotting. The intervention group presented a lower increase in CML compared to the control group (12.39% median change in the intervention vs. 69.34% in the control group, p = 0.013), while RAGE (% mean change −56.54 in the intervention vs. 46.51 in the control group, p < 0.001) and COX-2 (% mean change −37.76 in the intervention vs. 0.27 in the control group, p < 0.001) were reduced compared to the control group. sRAGE was reduced in both groups. In addition, HbA1c (at two months), total cholesterol, and triglycerides were reduced in the intervention versus the control group. The adoption of healthy cooking methods deserves further research as a possible way of modulating inflammatory markers in patients with CKD.
Agrimonia coreana Extract Exerts Its Therapeutic Effect through CRAC Channel Inhibition for Atopic Dermatitis Treatment
Authors: Kim Jintae , Lee Ji Min , Park Su Jin , Nam Yu Ran , Choi Seong Woo , Nam Joo Hyun , Kim Hyun Jong , Kim Woo Kyung
Published: August 2024

Atopic dermatitis (AD) is a common allergic inflammatory skin condition marked by severe itching, skin lichenification, and chronic inflammation. AD results from a complex immune response, primarily driven by T lymphocytes and environmental triggers, leading to a disrupted epidermal barrier function. Traditional treatments, such as topical corticosteroids, have limitations due to long-term side effects, highlighting the need for safer alternatives. Here, we aimed to show that Agrimonia coreana extract (ACext) can be used in treating AD-related dermatologic symptoms. ACext could inhibit CRAC (Calcium Release-Activated Calcium) channel activity, reducing Orai1/CRAC currents and decreasing intracellular calcium signaling. This inhibition was further confirmed by the reduced IL-2 levels and T cell proliferation upon ACext treatment. In a mouse model of AD, ACext significantly ameliorates symptoms, improves histological parameters, and enhances skin barrier function, demonstrating its potential for treating AD.
An Insight into the Mechanism of DNA Cleavage by DNA Endonuclease from the Hyperthermophilic Archaeon Pyrococcus furiosus
Authors: Davletgildeeva Anastasiia T. , Kuznetsova Aleksandra A. , Ishchenko Alexander A. , Saparbaev Murat , Kuznetsov Nikita A.
Published: August 2024

Hyperthermophilic archaea such as Pyrococcus furiosus survive under very aggressive environmental conditions by occupying niches inaccessible to representatives of other domains of life. The ability to survive such severe living conditions must be ensured by extraordinarily efficient mechanisms of DNA processing, including repair. Therefore, in this study, we compared kinetics of conformational changes of DNA Endonuclease Q from P. furiosus during its interaction with various DNA substrates containing an analog of an apurinic/apyrimidinic site (F-site), hypoxanthine, uracil, 5,6-dihydrouracil, the α-anomer of adenosine, or 1,N6-ethenoadenosine. Our examination of DNA cleavage activity and fluorescence time courses characterizing conformational changes of the dye-labeled DNA substrates during the interaction with EndoQ revealed that the enzyme induces multiple conformational changes of DNA in the course of binding. Moreover, the obtained data suggested that the formation of the enzyme–substrate complex can proceed through dissimilar kinetic pathways, resulting in different types of DNA conformational changes, which probably allow the enzyme to perform its biological function at an extreme temperature.
Hematopoietic Growth Factors Regulate the Entry of Monocytes into the Adult Brain via Chemokine Receptor CCR5
Authors: Ren Xuefang Sophie , He Junchi , Li Songruo , Hu Heng , Kyle Michele , Kohsaka Shinichi , Zhao Li-Ru
Published: August 2024

Monocytes are circulating macrophage precursors generated from bone marrow hematopoietic stem cells. In adults, monocytes continuously replenish cerebral border-associated macrophages under physiological conditions. Monocytes also rapidly infiltrate the brain in pathological settings. The mechanisms of recruiting monocyte-derived macrophages into the brain under pathological conditions have been extensively studied. However, it remains unclear how monocytes enter the brain to renew border-associated macrophages under physiological conditions. Using both in vitro and in vivo approaches, this study reveals that a combination of two hematopoietic growth factors, stem cell factor (SCF) and granulocyte colony-stimulating factor (G-CSF), complementarily and synergistically enhances the adhesion of monocytes to cerebral endothelial cells in a dose-dependent manner. Cysteine-cysteine chemokine receptor 5 (CCR5) in brain endothelial cells, but not the cell adhesion molecules mediating neuroinflammation-related infiltration of monocyte-derived macrophages, modulates SCF+G-CSF-enhanced monocyte-endothelial cell adhesion. Blocking CCR5 or genetically deleting CCR5 reduces monocyte-endothelial cell adhesion induced by SCF+G-CSF. The SCF+G-CSF-enhanced recruitment of bone marrow-derived monocytes/macrophages into the cerebral perivascular space is also reduced in adult CCR5 knockout mice. This study demonstrates the role of SCF and G-CSF in regulating the entry of monocytes into the adult brain to replenish perivascular macrophages.
Transcriptome Profiles Reveal Key Regulatory Networks during Single and Multifactorial Stresses Coupled with Melatonin Treatment in Pitaya (Selenicereus undatus L.)
Authors: Khokhar Aamir Ali , Hui Liu , Khan Darya , You Zhang , Zaman Qamar U , Usman Babar , Wang Hua-Feng
Published: August 2024

In response to evolving climatic conditions, plants frequently confront multiple abiotic stresses, necessitating robust adaptive mechanisms. This study focuses on the responses of Selenicereus undatus L. to both individual stresses (cadmium; Cd, salt; S, and drought; D) and their combined applications, with an emphasis on evaluating the mitigating effects of (M) melatonin. Through transcriptome analysis, this study identifies significant gene expression changes and regulatory network activations. The results show that stress decreases pitaya growth rates by 30%, reduces stem and cladode development by 40%, and increases Cd uptake under single and combined stresses by 50% and 70%, respectively. Under stress conditions, enhanced activities of H2O2, POD, CAT, APX, and SOD and elevated proline content indicate strong antioxidant defenses. We identified 141 common DEGs related to stress tolerance, most of which were related to AtCBP, ALA, and CBP pathways. Interestingly, the production of genes related to signal transduction and hormones, including abscisic acid and auxin, was also significantly induced. Several calcium-dependent protein kinase genes were regulated during M and stress treatments. Functional enrichment analysis showed that most of the DEGs were enriched during metabolism, MAPK signaling, and photosynthesis. In addition, weighted gene co-expression network analysis (WGCNA) identified critical transcription factors (WRKYs, MYBs, bZIPs, bHLHs, and NACs) associated with antioxidant activities, particularly within the salmon module. This study provides morpho-physiological and transcriptome insights into pitaya’s stress responses and suggests molecular breeding techniques with which to enhance plant resistance.
Assessment in the Age of Education 4.0: Unveiling Primitive and Hidden Parameters for Evaluation
Authors: Verma Anil , Kaur Parampreet , Singh Aman
Published: August 2024

This study delves into the nuanced aspects that influence the quality of education within the Education 4.0 framework. Education 4.0 epitomizes a contemporary educational paradigm leveraging IoT devices, sensors, and actuators to facilitate real-time and continuous assessment, thereby enhancing student evaluation methodologies. Within this context, the study scrutinizes the pivotal role of infrastructure, learning environment, and faculty, acknowledged as fundamental determinants of educational excellence. Identifying five discrete yet crucial hidden parameters, awareness, accessibility, participation, satisfaction, and academic loafing, this paper meticulously examines their ramifications within the Education 4.0 landscape. Employing a comparative analysis encompassing pre- and post-implementation scenarios, the research assesses the transformative impact of Education 4.0 on the educational sector while dissecting the influence of these hidden parameters across these temporal contexts. The findings underscore the substantial enhancements introduced by Education 4.0, including the provision of real-time and continuous assessment mechanisms, heightened accessibility to educational resources, and amplified student engagement levels. Notably, the study advocates for bolstering stakeholders’ accountability as a strategic measure to mitigate academic loafing within an ambient educational milieu. In essence, this paper offers invaluable insights into the intricate interplay between hidden parameters and educational quality, elucidating the pivotal role of Education 4.0 in catalyzing advancements within the education industry.
Genetic Characterization of Two Novel Insect-Infecting Negative-Sense RNA Viruses Identified in a Leaf Beetle, Aulacophora indica
Authors: Chen Meng-Nan , Ye Zhuang-Xin , Feng Ke-Hui , Yuan Jing-Na , Chen Jian-Ping , Zhang Chuan-Xi , Li Jun-Min , Mao Qian-Zhuo
Published: August 2024

Herbivorous insects harbor a variety of insect-specific viruses (ISVs) some of which are considered to be valuable biological agents for potential applications in biological defense and control strategies. Leaf beetles with chewing mouthparts are particularly known for their capacity to disrupt plant tissue while feeding, often creating openings that can act as entry points for plant pathogens. In this study, we have identified two new negative-sense RNA viruses infecting the leaf beetle Aulacophora indica, an important member of the Chrysomelidae family. These recently discovered viruses belong to the viral families Nyamiviridae and Chuviridae and have been preliminarily named Aulacophora indica nyami-like virus 1 (AINlV1) and Aulacophora indica chu-like virus 1 (AIClV1), respectively. The complete genomic sequences of these viruses were obtained using rapid amplification of cDNA ends (RACE) techniques. Detailed analysis of their genomic structures has confirmed their similarity to other members within their respective families. Furthermore, analysis of virus-derived small interfering RNA (vsiRNA) demonstrated a high abundance and typical vsiRNA pattern of AINlV1 and AIClV1, offering substantial evidence to support their classification as ISVs. This research enhances our understanding of viral diversity within insects.
Prenatal Diagnosis of Cleft Lip and Palate: A Retrospective Study
Authors: Brăila Anca Daniela , Damian Constantin Marian , Albu Cristina-Crenguţa , Botoacă Oana , Dȋră Laurențiu Mihai , Albu Ştefan-Dimitrie , Brăila Matei Georgian , Bănățeanu Andreea-Mariana , Poalelungi Cristian-Viorel , Bogdan-Andreescu Claudia Florina
Published: August 2024

Cleft lip and/or palate are prevalent congenital anomalies. Early and accurate diagnosis allows proper case management. The Objective: This retrospective cohort study aimed to investigate the association between cleft lip and palate and other congenital anomalies. Methods: This study analyzed 17 pregnancies prenatally diagnosed with cleft lip and palate. The investigations consisted of ultrasound examination, fetal karyotyping through amniocentesis, and family tree analysis. In the presence of an abnormal fetal karyotype, the parental karyotype was also indicated. Results: Of the 17 cases identified, 9 (52.94%) were syndromic and 8 (47.06%) were non-syndromic. The genetic syndromes identified in association with cleft lip and palate in this study included translocation syndrome (one case), Patau syndrome, trisomy 13 (seven cases), and Edwards syndrome, mosaic trisomy 18 (one case). Conclusions: A comprehensive approach ensures a thorough assessment and accurate diagnosis. Early detection and a multidisciplinary approach allow appropriate case management.
To Shrink or Not to Shrink? An Objective Assessment of Free Gracilis Muscle Volume Change in Lower-Extremity Defect Reconstruction
Authors: Koban Konstantin Christoph , Kuhlmann Constanze , Wachtel Nikolaus , Hirschmann Maximilian , Hellweg Marc , Karcz Konrad Wojcieck , Giunta Riccardo Enzo , Ehrl Denis
Published: August 2024

Background: The use of free gracilis muscle flaps in reconstructive surgery of the lower leg is common practice to cover defects. However, there is still a lack of understanding of the morphometric changes that occur in the transferred muscle and area of interest over time, particularly the characteristic volume decrease that is observed over the course of the first year. This study aimed to assess volume changes in patients with free gracilis muscle flap reconstruction following infection, trauma, or malignancies of the lower extremity. Methods: Three-dimensional surface imaging was performed intraoperatively after 2 weeks, 6 months, and 12 months with the Vectra H2 system. A total of 31 patients were included in this study and analyzed. Results: There was an average volume increase of 146.67 ± 29.66% 2 weeks after reconstruction. Compared to this volume increase, there was a reduction of 108.44 ± 13.62% after 12 months (p < 0.05). Overall, we found a shrinkage to 85.53 ± 20.14% of the intraoperative baseline volume after 12 months. Conclusions: The use of non-invasive 3D surface imaging is a valuable tool for volume monitoring after free flap reconstruction of the lower extremity. The free gracilis muscle flap undergoes different phases of volume change over the first year, with the greatest influence on overall change being the development and decongestion of edema. Precise initial surgical tailoring is crucial for optimal long-term functional and cosmetic results.
High-Dose Neutrophil-Depleted Platelet-Rich Plasma Therapy for Knee Osteoarthritis: A Retrospective Study
Authors: De Matthaeis Andrea , Bianchi Maria , Putzulu Rossana , Maccauro Giulio
Published: August 2024

Background/Objectives: Encouraging results have been reported for Platelet-Rich Plasma (PRP) treatment for knee osteoarthritis (KOA). This study reports the efficacy and safety of a high dose of neutrophile and red-blood-cell-depleted PRP to treat patients with KOA. Methods: A total of 212 consecutive patients diagnosed with Kellgren–Lawrence (KL) grading 1–3 KOA chronic knee pain for at least 1 year were treated with three injections at 15-day intervals with a high dose of neutrophil-depleted PRP (4 billion platelets). Clinical outcomes were retrospectively recorded as the percentage of responders at 3-, 6-, and 12-month follow-up, following the OMERACT-OARSI criteria. Pain, through the VAS score and WOMAC score, was also been recorded. Results: A total of 4 mL of PRP containing 4 × 109 platelets was obtained by single-spin centrifugation and injected intra-articularly into each patient with no preactivation. The overall responder rate of patients responding to the OMERACT-OARSI criteria at 3, 6, and 12 months was 68.9%, 72.7%, and 70.6%, respectively. A significant improvement in VAS and WOMAC scores at 3-, 6-, and 12-month follow-up compared to the pretreatment value (p < 0.01) was observed. The lowest VAS score was observed at 6 months overall and in all three KL-graded groups. The KL2 groups showed the best results regarding pain reduction and their WOMAC score at 6 months (p < 0.01). Conclusions: For KL1–3 KOA, a high dosage of neutrophil-depleted PRP is a successful treatment. It has long-lasting effects that last up to one year, relieves symptoms, and may slow the advancement of the disease.
Artificial Intelligence for the Automatic Diagnosis of Gastritis: A Systematic Review
Authors: Turtoi Daria Claudia , Brata Vlad Dumitru , Incze Victor , Ismaiel Abdulrahman , Dumitrascu Dinu Iuliu , Militaru Valentin , Munteanu Mihai Alexandru , Botan Alexandru , Toc Dan Alexandru , Duse Traian Adrian , Popa Stefan Lucian
Published: August 2024

Background and Objective: Gastritis represents one of the most prevalent gastrointestinal diseases and has a multifactorial etiology, many forms of manifestation, and various symptoms. Diagnosis of gastritis is made based on clinical, endoscopic, and histological criteria, and although it is a thorough process, many cases are misdiagnosed or overlooked. This systematic review aims to provide an extensive overview of current artificial intelligence (AI) applications in gastritis diagnosis and evaluate the precision of these systems. This evaluation could highlight the role of AI as a helpful and useful tool in facilitating timely and accurate diagnoses, which in turn could improve patient outcomes. Methods: We have conducted an extensive and comprehensive literature search of PubMed, Scopus, and Web of Science, including studies published until July 2024. Results: Despite variations in study design, participant numbers and characteristics, and outcome measures, our observations suggest that implementing an AI automatic diagnostic tool into clinical practice is currently feasible, with the current systems achieving high levels of accuracy, sensitivity, and specificity. Our findings indicate that AI outperformed human experts in most studies, with multiple studies exhibiting an accuracy of over 90% for AI compared to human experts. These results highlight the significant potential of AI to enhance diagnostic accuracy and efficiency in gastroenterology. Conclusions: AI-based technologies can now automatically diagnose using images provided by gastroscopy, digital pathology, and radiology imaging. Deep learning models exhibited high levels of accuracy, sensitivity, and specificity while assessing the diagnosis, staging, and risk of neoplasia for different types of gastritis, results that are superior to those of human experts in most studies.
Explorative Identification of Anatomical Parameters Associated with Successful Pessary Fitting in Pelvic Organ Prolapse Using Dynamic Magnetic Resonance Imaging
Authors: Triepels Charlotte P. R. , Boogaard Lars L. , Fütterer Jurgen J. , van Kuijk Sander M. J. , Spaans Wilbert A. , Kruitwagen Roy F. P. M. , Weemhoff Mirjam , Notten Kim J. B.
Published: August 2024

Background: Pelvic organ prolapse (POP) affects many women and is often managed with pessary treatment, yet predicting the success of fitting remains challenging. This study aims to identify anatomical parameters associated with successful and unsuccessful pessary treatment using dynamic magnetic resonance imaging (dMRI). Methods: A cross-sectional study in Maastricht University Medical Centre (MUMC+), the Netherlands. Sixteen women with a cystocele and/or descensus uteri minimal POP-Q stage 2, using pessary treatment, were included. All women underwent a dynamic MRI of the pelvic floor at rest, during contraction and on Valsalva. The anatomical parameters evaluated included various lengths and angles. The association between the anatomical parameters and pessary fitted is assessed using partial least squares regression. The predictive accuracy was tested using cross-validation based on the partial least squares model with the most important variables. Results: Seven of the sixteen women (43.8%) were in the non-fitting group (due to movement, rotation or expulsion of the pessary), and nine women (56.3%) were in the fitting group. Participants in the non-fitting group had a significantly lower body mass index (BMI). Variables such as total vaginal length (TVL) and certain angles were highly predictive of pessary fitting success, with variable importance of projection (VIP) scores indicating their importance. The prediction models showed accuracies ranging from 53.3% to 80.0%. Conclusions: In this explorative study, TVL, cervical length (CL), sacrococcygeal angle and pubococcygeal angle were key variables associated with pessary fitting success. These findings offer valuable insights for optimizing pessary fitting procedures and the development of new pessaries.
Major Causes of Conflicting Interpretations of Variant Pathogenicity in Rare Disease: A Systematic Analysis
Authors: Lazareva Tatyana E. , Barbitoff Yury A. , Nasykhova Yulia A. , Glotov Andrey S.
Published: August 2024

The identification of the genetic causes of inherited disorders from next-generation sequencing (NGS) data remains a complicated process, in particular due to challenges in interpretation of the vast amount of generated data and hundreds of candidate variants identified. Inconsistencies in variant classification, where genetic centers classify the same variant differently, can hinder accurate diagnoses for rare diseases. Publicly available databases that collect data on human genetic variations and their association with diseases provide ample opportunities to discover conflicts in variant interpretation worldwide. In this study, we explored patterns of variant classification discrepancies using data from ClinVar, a public archive of variant interpretations. We found that 5.7% of variants have conflicting interpretations (COIs) reported, and the vast majority of interpretation conflicts arise for variants of uncertain significance (VUS). As many as 78% of clinically relevant genes harbor variants with COIs, and genes with high COI rates tended to have more exons and longer transcripts, with a greater proportion of genes linked to several distinct conditions. The enrichment analysis of COI-enriched genes revealed that the products of these genes are involved in cardiac disorders, muscle development, and function. To improve diagnoses, we believe that specific variant interpretation rules could be developed for such genes. Additionally, our findings underscore the need for the publication of variant pathogenicity evidence and the importance of considering every variant as VUS unless proven otherwise.
Sustainable Utilization and Conservation through Compensation for Ecosystem Services: Experiences from the Koga Watershed in Northern Ethiopia
Authors: Senbeta Feyera , Abi Meskerem , Tolossa Degefa , Abate Esubalew , Terefe Getachew
Published: August 2024

Payments for ecosystem services have recently been introduced into the ecosystem management landscape to counter ecosystem degradation. This study examines the experiences of sustainable use and management of watersheds using a compensation for ecosystem service model in the Koga watershed, Northern Ethiopia. The study primarily employed qualitative methods. Thirteen informants, chosen from a group of project implementers, were contacted for telephone interviews, and a document analysis was also conducted. Thematic approaches were employed to analysis the data. The study findings revealed that a project had been introduced to implement an innovative payment/compensation for ecosystem services (CES) scheme to manage watersheds and to alleviate poverty in the watershed. The scheme connected upstream and downstream communities and cooperatives through CES, earning praise for its effectiveness in combating dam siltation and watershed degradation. The initiative also established various enabling conditions, such as capacity building, bylaw formulation, and payment procedures for beneficiary communities. However, the CES scheme still has a long way to go to deliver the anticipated positive impacts on local livelihoods and watershed management. It is believed that a comprehensive legal framework, a stable political and social environment, and sustainable financing mechanisms are crucial for the success of any CES project. Therefore, Ethiopia should strive to meet these requirements to ensure that any future CES projects are designed and implemented sustainably.
Long-Term Effect of Tillage Systems on Planosol Physical Properties, CO2 Emissions and Spring Barley Productivity
Authors: Sinkevičienė Aušra , Romaneckas Kęstutis , Jackevičienė Karolina , Petrikaitė Toma , Balandaitė Jovita , Kimbirauskienė Rasa
Published: August 2024

As the population grows, more food is needed to keep the food supply chain running smoothly. For many years, intensive farming systems have been used to meet this need. Currently, due to intense climate change and other global natural problems, there is a shift towards sustainable use of natural resources and simplified methods of tillage. Soil tillage intensity influences the distribution of nutrients, and soil’s physical and mechanical properties, as well as gas flows. The impact of reduced tillage on these indices in spring barley cultivation is still insufficient and requires more analysis on a global scale. This study was carried out at Vytautas Magnus University, Agriculture Academy (Lithuania) in 2022–2023. The aim of the investigation was to determine the effect of the tillage systems on the soil temperature, moisture content, CO2 respiration and concentration in spring barley cultivation. Based on a long-term tillage experiment, five tillage systems were tested: deep and shallow moldboard ploughing, deep cultivation-chiseling, shallow cultivation-chiseling, and no tillage Shallow plowing technology has been found to better conserve soil moisture and maintain higher temperatures in most cases. During almost the entire study period, the spring barley crop with deep cultivation had lower moisture content and lower soil temperature. Shallow cultivation fields in most cases increased CO2 emissions and CO2 concentration. When applying direct sowing to the uncultivated soil (10–20 cm), the concentration of CO2 decreased from 0.01 to 0.148 percent. pcs. The results show that in direct sowing fields, most cases had a positive effect on crop density. Direct sowing fields resulted in significantly lower, from 7.9 to 26.5%, grain yields of spring barley in the years studied.
Assessing the Impact of Land Conversion on Carbon Stocks and GHG Emissions
Authors: Ituen Ima , Hu Baoxin
Published: August 2024

With the recent thrust to convert forests in Ontario’s Clay Belt to agricultural land, a vital need arises to assess the attendant effects on carbon and greenhouse gas (GHG) emissions. This paper examines the possible effect of land conversion on soil organic carbon and GHG emissions within a study area in Northern Ontario, Canada, during the next two decades under different land management schemes. The study established a framework to conduct simulations with the DNDC model for agricultural lands and the CBM for forested areas. The methodology involves a unique change detection method for models’ land cover and disturbance inputs. The work highlights the improvement in carbon simulation accuracy from better inputs to carbon models. Furthermore, it addresses modalities to ensure fewer uncertainties are introduced while merging data from multiple geospatial data sources. The simulations demonstrated that the carbon sequestration potential in the forests was almost double the soil organic carbon accumulation in the agricultural lands. Validations done for the estimation of carbon sequestered included comparisons of the carbon model outputs from field survey data from 2018–2021. In most sites, the carbon amounts from the computer models compared to those from the field survey, within limits of error. The average uncertainties in GHG emissions ranged from ~0.5% to 12.8%.
Duloxetine-Induced Antidiuresis in Rats with Lithium-Induced Nephrogenic Diabetes Insipidus
Authors: Kim Sua , Jo Chor Ho , Kim Gheun-Ho
Published: August 2024

Antidepressants, including duloxetine, are a significant cause of drug-induced hyponatremia, which can disrupt the continuation of medication. Tolvaptan is beneficial for correcting hyponatremia caused by the syndrome of inappropriate antidiuresis, but its impact on duloxetine-induced hyponatremia remains unknown. We used male Sprague-Dawley rats to examine the impact of duloxetine treatment on lithium-induced nephrogenic diabetes insipidus (Li-NDI) and to evaluate whether the results were reversed by co-treatment with tolvaptan. To induce Li-NDI, lithium chloride (40 mmol lithium/kg dry food) was administered for 2 weeks. Duloxetine (50 mg/kg/day) and tolvaptan (10 mg/kg/day) were also administered in food to assess their individual effects over the same period. At the end of each animal experiment, kidneys were harvested to measure levels of cAMP, vasopressin-2 receptor (V2R), cAMP-responsive element binding protein 1 (CREB-1), aquaporin-2 (AQP2), and prostaglandin E2 (PGE2). Water diuresis was induced in the Li-NDI rats, and duloxetine treatment reduced polyuria while increasing urine osmolality. Duloxetine treatment prevented the decrease in total AQP2, AQP2 phosphorylation at serine 256, and CREB-1 phosphorylation in Li-NDI rats. The V2R mRNA level was also reduced in Li-NDI rats and restored by duloxetine treatment. In the subsequent experiment, the decreased water diuresis in Li-NDI rats treated with duloxetine was reversed by co-treatment with tolvaptan. Tolvaptan co-treatment also reversed the changes in AQP2 protein and CREB-1 phosphorylation in the renal cortex and medulla. The decreased cAMP levels in Li-NDI rat kidneys were elevated by duloxetine treatment, and this elevation was reversed by co-treatment with tolvaptan. However, the elevated PGE2 levels in Li-NDI rat kidneys were not affected by either duloxetine alone or tolvaptan co-treatment. In conclusion, antidiuresis was induced by duloxetine in Li-NDI and reversed by tolvaptan co-treatment through alterations in the V2R-cAMP-AQP2 pathway. These findings could underlie the mechanism of duloxetine-induced hyponatremia and suggest the potential usefulness of tolvaptan in treating drug-induced hyponatremia.
Bovine Mineral Grafting Affects the Hydrophilicity of Dental Implant Surfaces: An In Vitro Study
Authors: Newman Allyson , Anderson Nina K. , Romanos Georgios E.
Published: August 2024

Wettability is recognized as an important property of implant surfaces for ensuring improved biological responses. However, limited information exists on how bone grafting procedures including materials influence the hydrophilic behavior of implant surfaces. This in vitro study aimed to investigate the influence of two bovine grafting materials after hydration on the wettability of four different disk surfaces: commercially pure titanium (CP-Ti), titanium–zirconium dioxide (TiZrO2-Cerid®), zirconia (SDS®), and niobium. Wettability tests were performed on each of the four implant surfaces with a solution of 0.9% sodium chloride after mixture with W-boneTM (Group A) or Bio-Oss® (Group B) or 0.9% sodium chloride alone (Group C). In total, 360 contact angle measurements were completed with n = 30 per group. Statistical analysis was performed using a one-way analysis with variance (ANOVA) test with a significant mean difference at the 0.05 level. For pure titanium, Group A demonstrated increased hydrophilicity compared to Group B. Both TiZrO2 and zirconia showed significant differences for Groups A, B and C, exhibiting a decrease in hydrophilicity after the use of bovine grafting materials compared to titanium surfaces. Niobium remained consistently hydrophobic. In summary, this study revealed that bovine grafting materials may diminish the hydrophilicity of zirconia surfaces and exert varied effects on titanium and niobium. These findings contribute to the understanding of implant surface interactions with grafting materials, offering insights for optimizing biological responses in implantology.
Long-Term Evaluation of a Ternary Mixture of Molten Salts in Solar Thermal Storage Systems: Impact on Thermophysical Properties and Corrosion
Authors: Henríquez Mauro , Reinoso-Burrows Juan Carlos , Pastén Raúl , Soto Carlos , Duran Carlos , Olivares Douglas , Guerreiro Luis , Cardemil José Miguel , Galleguillos Madrid Felipe M. , Fuentealba Edward
Published: August 2024

Solar thermal plants typically undergo trough operational cycles spanning between 20 and 25 years, highlighting the critical need for accurate assessments of long-term component evolution. Among these components, the heat storage media (molten salt) is crucial in plant design, as it significantly influences both the thermophysical properties of the working fluid and the corrosion of the steel components in thermal storage systems. Our research focused on evaluating the long-term effects of operating a low-melting-point ternary mixture consisting of 30 wt% LiNO3, 57 wt% KNO3, and 13 wt% NaNO3. The ternary mixture exhibited a melting point of 129 °C and thermal stability above 550 °C. Over 15,000 h, the heat capacity decreased from 1.794 to 1.409 J/g °C. Additionally, saline components such as CaCO3 and MgCO3, as well as lithium oxides (LiO and LiO2), were detected due to the separation of the ternary mixture. A 30,000 h exposure resulted in the formation of Fe2O3 and the presence of Cl, indicating prolonged interaction with the marine environment. This investigation highlights the necessity of analyzing properties under actual operating conditions to accurately predict the lifespan and select the appropriate materials for molten salt-based thermal storage systems.
Multifunctional Design of Catalysts for Seawater Electrolysis for Hydrogen Production
Authors: Cui Chenmeng , Zhang Haonan , Wang Dan , Song Jihuan , Yang Ying
Published: August 2024

Direct seawater electrolysis is a promising technology within the carbon-neutral energy framework, leveraging renewable resources such as solar, tidal, and wind energy to generate hydrogen and oxygen without competing with the demand for pure water. High-selectivity, high-efficiency, and corrosion-resistant multifunctional electrocatalysts are essential for practical applications, yet producing stable and efficient catalysts under harsh conditions remains a significant challenge. This review systematically summarizes recent advancements in advanced electrocatalysts for seawater splitting, focusing on their multifunctional designs for selectivity and chlorine corrosion resistance. We analyze the fundamental principles and mechanisms of seawater electrocatalytic reactions, discuss the challenges, and provide a detailed overview of the progress in nanostructures, alloys, multi-metallic systems, atomic dispersion, interface engineering, and functional modifications. Continuous research and innovation aim to develop efficient, eco-friendly seawater electrolysis systems, promoting hydrogen energy application, addressing efficiency and stability challenges, reducing costs, and achieving commercial viability.
Recent Progress of Iron-Based Magnetic Absorbers and Its Applications in Elastomers: A Review
Authors: Xu Wanting , Liu Na , Lu Zhongchen
Published: August 2024

As a result of continuing scientific and technological progress, electromagnetic waves have become increasingly pervasive across a variety of domains, particularly within the microwave frequency range. These waves have found extensive applications in wireless communications, high-frequency electronic circuits, and several related fields. As a result, absorptive materials have become indispensable for dual-use applications across both the military and civilian domains because of their exceptional electromagnetic wave absorption properties. This paper, beginning with the operating mechanisms of absorptive materials, aims to provide an overview of the strategies that have been used to enhance the absorption performance of iron-based magnetic absorbers (IBMAs) and discuss the current research status of absorptive material components. The fabrication of a ferromagnetic absorber in terms of morphology, heterointerface coupling, and macrostructural enhancements and the effect of powder characteristics on their electromagnetic properties are discussed. Additionally, the application of IBMAs in elastomers is summarized. Finally, this paper summarizes the limitations of existing ferromagnetic absorber materials and offers a perspective on their potential future developments. The objective of the ongoing research is to fabricate absorptive components that have thin profiles, lightweight construction, wide absorption frequency ranges, and strong absorption capabilities.
Evaluation of Passive Films on 17-7PH and 410 Stainless Steel Exposed to NaCl Solution
Authors: Martínez-Aparicio Brisa , Gaona-Tiburcio Citlalli , Almeraya-Calderon Facundo , Goldsberry Reece , Castaneda Homero
Published: August 2024

This work covers the formation of a passive state for two different alloys used in the aeronautical industry. The aim of this study is to investigate the effectiveness of passivation treatments on 17-7PH and 410 SS (stainless steel) samples, specifically when performed with citric and nitric acid solutions at 49 °C using an immersion time of 90 min and subsequent exposure in 3.5 wt.% NaCl solution. Employing the cyclic potentiodynamic polarization (CPP) technique, the corrosion properties of the passivated material were evaluated according to the ASTM G65-11 standard. A microstructural analysis was performed using scanning electron microscopy (SEM). The passivated layer was characterized via X-ray photoelectron spectroscopy. In the results, the CPP curves showed positive hysteresis, indicating pitting localized corrosion, and 17-7PH steel passivated at 49 °C for 90 min in citric acid exhibited lower corrosion rate values equivalent to ×10−3 mm/year.
A Velocity-Adaptive MPC-Based Path Tracking Method for Heavy-Duty Forklift AGVs
Authors: Wang Yajun , Sun Kezheng , Zhang Wei , Jin Xiaojun
Published: August 2024

In warehouses with vast quantities of heavy goods, heavy-duty forklift Automated Guided Vehicles (AGVs) play a key role in facilitating efficient warehouse automation. Due to their large load capacity and high inertia, heavy-duty forklift AGVs struggle to automatically navigate optimized routes. Additionally, rapid acceleration and deceleration can pose safety hazards. This paper proposes a velocity-adaptive model predictive control (MPC)-based path tracking method for heavy-duty forklift AGVs. The movement of heavy-duty forklift-type AGVs is categorized into straight-line and curve-turning motions, corresponding to the straight and curved sections of the reference path, respectively. These sections are segmented based on their curvature. The best driving speeds for straight and curved sections were 1.5 m/s and 0.3 m/s, respectively, while the optimal acceleration rates were 0.2 m/s2 for acceleration and −0.2 m/s2 for deceleration in straight paths and 0.3 m/s2 for acceleration with −0.15 m/s2 for deceleration in curves. Moreover, preferred sampling times, prediction domain, and control domain were determined through simulations at various speeds. Four path tracking methods, including pure tracking, Linear Quadratic Regulator (LQR), MPC, and the velocity-adaptive MPC, were simulated and evaluated under straight-line, turning, and complex double lane change conditions. Field experiments conducted in a warehouse environment demonstrated the effectiveness of the proposed path tracking method. Findings have implications for advancing path tracking control in narrow aisles.
Influence of Atomizing Gas Pressure on Microstructure and Properties of Nickel Silicide Intended for Additive Manufacturing
Authors: Ibrahim Mohammad , Gobber Federico Simone , Hulme Christopher , Grasmo Geir , Aune Ragnhild E.
Published: August 2024

Nickel silicides are crucial in advanced technology applications ranging from semiconductor devices to high-temperature materials. Gas atomization is a process that involves the formation of fine liquid droplets and their rapid cooling and solidification to make powder particles. The final microstructure and the properties of the particles are highly sensitive to the gas atomization process parameters. In the present study, gas atomization of NiSi12-wt% was performed at three different pressures (35, 40, and 45 bars) to optimize the particle size distribution for additive manufacturing applications. A comprehensive range of characterization techniques, including scanning electron microscopy, X-ray diffraction, particle size distribution measurements, light optical microscopy, and density measurements, was used to evaluate the microstructural features, phase composition, and density of the produced NiSi12-wt% powders. Higher atomizing gas pressures resulted in a finer particle size distribution due to improved molten droplet breakup, increased satellite formation, and a well-suited particle size distribution for additive manufacturing applications.
Multi-Channel Microscale Nerve Cuffs for Spatially Selective Neuromodulation
Authors: Riley Morgan , Tala FNU , Johnson Katherine J. , Johnson Benjamin C.
Published: August 2024

Peripheral nerve modulation via electrical stimulation shows promise for treating several diseases, but current approaches lack selectivity, leading to side effects. Exploring selective neuromodulation with commercially available nerve cuffs is impractical due to their high cost and limited spatial resolution. While custom cuffs reported in the literature achieve high spatial resolutions, they require specialized microfabrication equipment and significant effort to produce even a single design. This inability to rapidly and cost-effectively prototype novel cuff designs impedes research into selective neuromodulation therapies in acute studies. To address this, we developed a reproducible method to easily create multi-channel epineural nerve cuffs for selective fascicular neuromodulation. Leveraging commercial flexible printed circuit (FPC) technology, we created cuffs with high spatial resolution (50 μm) and customizable parameters like electrode size, channel count, and cuff diameter. We designed cuffs to accommodate adult mouse or rat sciatic nerves (300–1500 μm diameter). We coated the electrodes with PEDOT:PSS to improve the charge injection capacity. We demonstrated selective neuromodulation in both rats and mice, achieving preferential activation of the tibialis anterior (TA) and lateral gastrocnemius (LG) muscles. Selectivity was confirmed through micro-computed tomography (μCT) and quantified through a selectivity index. These results demonstrate the potential of this fabrication method for enabling selective neuromodulation studies while significantly reducing production time and costs compared to traditional approaches.
Self-Assembly Regulated Photocatalysis of Porphyrin-TiO2 Nanocomposites
Authors: Liu Yisheng , Lv Xinpeng , Zhong Yong , Wang Gaoyang , Liu Shuanghong , Chen Sudi , Qi Cai , He Mu , Shangguan Ping , Luo Zhengqun , Li Xi , Guo Jincheng , Sun Jiajie , Bai Feng , Wang Jiefei
Published: August 2024

Photoactive artificial nanocatalysts that mimic natural photoenergy systems can yield clean and renewable energy. However, their poor photoabsorption capability and disfavored photogenic electron–hole recombination hinder their production. Herein, we designed two nanocatalysts with various microstructures by combining the tailored self-assembly of the meso-tetra(p-hydroxyphenyl) porphine photosensitizer with the growth of titanium dioxide (TiO2). The porphyrin photoabsorption antenna efficiently extended the absorption range of TiO2 in the visible region, while anatase TiO2 promoted the efficient electron–hole separation of porphyrin. The photo-induced electrons were transferred to the surface of the Pt co-catalyst for the generation of hydrogen via water splitting, and the hole was utilized for the decomposition of methyl orange dye. The hybrid structure showed greatly increased photocatalytic performance compared to the core@shell structure due to massive active sites and increased photo-generated electron output. This controlled assembly regulation provides a new approach for the fabrication of advanced, structure-dependent photocatalysts.