bluuebunny/crossref_metadata_2025 huggingface dataset:
Fresh Validation of the Low Carbon Development Hypothesis under the EKC Scheme in Portugal, Italy, Greece and Spain
Authors: Balsalobre-Lorente Daniel , Leitão Nuno Carlos , Bekun Festus Victor
Published: January 2021

The present study is in line with the United Nations Sustainable Development Goals (UN-SDGs) that address pertinent global issues. This study focuses on the need for access to clean and affordable energy consumption, responsible energy consumption, sustainable economic growth, and climate change mitigation. To this end, this paper evaluates the relevance of the renewable energy sector on the environmental Kuznets curve (EKC) framework in Portugal, Italy, Greece, and Spain for the period 1995–2015. As an econometric strategy, we adopt the use of panel data over the highlighted countries. In the first step, we apply the unit root test recommended by Levin, Lin, and Chu in conjunction with ADF-Fisher, and Phillips-Perron for robustness and consistency. We found that the variables used in this study are integrated I (1) in the first difference. In the second step, we apply the Pedroni cointegration test, and Kao Residual cointegration test, and we observe that the variables are cointegrated in the long run. The generalized least squares (GLS), the panel fully modified least squares (FMOLS), ordinary least squares robust (OLS), and panel quantile regression are considered in this research. The econometric results validate the assumption of the environmental Kuznets curve, i.e., and there is a positive correlation between income per capita and a negative effect of squared income per capita on carbon dioxide emissions. In contrast, we observe that renewable energy reduces CO2 emissions. Finally, we also find a direct connection between the urban population and the environmental degradation in the examined blocs. These results show that in Portugal, Italy, Greece, and Spain, more is required to achieve environmental sustainability in the respective countries growth trajectory. Further policy prescriptions are appended in the concluding section of this study.
Combining the Digital, Social and Physical Layer to Create Age-Friendly Cities and Communities
Authors: Pedell Sonja , Borda Ann , Keirnan Alen , Aimers Nicole
Published: January 2021

This qualitative investigation makes suggestions about creating age-friendly cities for older adults focusing on three domains of the World Health Organization (WHO) age-friendly city framework namely “Communication and Information”, “Outdoor Spaces and Buildings” and “Social Participation”. The authors present two case studies, the first one focusing on older adults using activity wearables for health self-management in the neighborhood, and the second one focusing on older adults engaged in social prescribing activities in the community. The authors then reflect on the relationships of the domains and future opportunities for age-friendly cities. These case studies apply a co-design and citizen-based approach focusing within these larger frameworks on emotions, values and motivational goals of older adults. Results suggest how the convergence of the often siloed age-friendly city components based on older adults’ goals and input can lead to better social participation and longer-term health outcomes. The authors propose that the digital, physical and social aspects need to be considered in all domains of age-friendly cities to achieve benefits for older adults. Further work involving older adults in the future shaping of age-friendly neighborhoods and cities, and identifying barriers and opportunities is required.
Prevalence and Antimicrobial Susceptibility Profiles of Microorganisms Associated with Lower Reproductive Tract Infections in Women from Southern Poland—Retrospective Laboratory-Based Study
Authors: Wójkowska-Mach Jadwiga , Pomorska-Wesołowska Monika , Romanik Małgorzata , Romaniszyn Dorota
Published: January 2021

Objective: Female infections affecting the genital tract include sexually transmitted diseases, endogenous infections such as vulvovaginal candidiasis, bacterial vaginosis (BV) or aerobic vaginitis (AV) and healthcare-associated infections. The aim of the study was to analyze the etiological factors of the vaginal dysbacteriosis, and the antimicrobial susceptibility of the dominant bacterial and fungal infections in different age groups of outpatient women from the Silesian Region. Materials and methods: A retrospective laboratory-based multi-center study encompassed 4994 women of different ages in Silesian Voivodeship, in the south of Poland; patients who had vaginal swabs collected as per physicians’ orders during the period from 1 January 2017 until 30 June 2018 were included in the study. The inclusion criteria were: non-hospitalized female, aged ≤80, with suspected vulvovaginal candidiasis or bacterial vaginosis and clinical sings of infections. Results: Gram-positive cocci were the ones most often isolated: Enterococcus faecalis (29.2%) and Streptoccoccus agalactiae (13.1%), followed by bacilli from the Enterobacteriaceae group, including Escherichia coli (26.3%). The presence of Streptococcus agalactiae was confirmed in 13.1%, slightly more often in the 45–80 age group, and Gardnerella vaginalis in 6.4%, most often in women aged 15–24. The prevalence of yeast-like infections was 24.3%, Candida albicans accounted for 78.3%, whereas among C. non-albicans spp.—C. glabrata dominated (14.9%) followed by C. parapsilosis (3.8%). The highest resistance was observed only in Streptococcus agalactiae as the MLSB mechanism (Macrolide-lincosamide-streptogramin B) was identified in 38.6% of strains. The prevalence of vulvovaginal candidiasis was 24.3%, the highest in women aged 15–44. Conclusions: Drug resistance in studied vulvovaginitis was associated only with Streptococcus agalactiae. A high proportion of yeast-like aetiology was found, probably associated with recurrent infections. In the analyzed cases only the Amsel criteria and culture methods were used for diagnosis without preparations and microbiological Nugent criteria.
Fruit and Vegetable Intake and All-Cause Mortality in a Chinese Population: The China Health and Nutrition Survey
Authors: Gu Yuxuan , He Yansu , Ali Shahmir H. , Harper Kaitlyn , Dong Hengjin , Gittelsohn Joel
Published: January 2021

This study was to investigate the association of long-term fruit and vegetable (FV) intake with all-cause mortality. We utilized data from the China Health and Nutrition Survey (CHNS), a prospective cohort study conducted in China. The sample population included 19,542 adult respondents with complete mortality data up to 31 December 2011. Cumulative FV intake was assessed by 3 day 24 h dietary recalls. Cox proportional hazards regression was used to estimate hazard ratios (HRs) and 95% confidence intervals (CIs) of all-cause mortality. Covariates included sociodemographic characteristics, lifestyle factors, health-related factors, and urban index. A total of 1409 deaths were observed during follow-up (median: 14 years). In the fully adjusted model, vegetable intake of the fourth quintile (327~408 g/day) had the greatest negative association with death compared to the lowest quintile (HR = 0.63, 95% CI: 0.53–0.76). Fruit intake of the fifth quintile (more than 126 g/day) had the highest negative association (HR = 0.24, 95% CI: 0.15–0.40) and increasing general FV intake were also negatively associated with all-cause mortality which demonstrated the greatest negative association in the amount of fourth quintile (HR = 0.59, 95% CI: 0.49–0.70) compared to the lowest quintile. To conclude, greater FV intake is associated with a reduced risk of total mortality for Chinese adults. High intake of fruit has a stronger negative association with mortality than differences in intake of vegetables. Our findings support recommendations to increase the intake of FV to promote overall longevity.
Role of Peroxisome Proliferator-Activated Receptors (PPARs) in Trophoblast Functions
Authors: Peng Lin , Yang Huixia , Ye Yao , Ma Zhi , Kuhn Christina , Rahmeh Martina , Mahner Sven , Makrigiannakis Antonis , Jeschke Udo , von Schönfeldt Viktoria
Published: January 2021

Peroxisome proliferator-activated receptors (PPARα, PPARβ/δ, and PPARγ) belong to the transcription factor family, and they are highly expressed in all types of trophoblast during pregnancy. The present review discusses currently published papers that are related to the regulation of PPARs via lipid metabolism, glucose metabolism, and amino acid metabolism to affect trophoblast physiological conditions, including differentiation, maturation, secretion, fusion, proliferation, migration, and invasion. Recent pieces of evidence have proven that the dysfunctions of PPARs in trophoblast lead to several related pregnancy diseases such as recurrent miscarriage, preeclampsia, intrauterine growth restriction, and gestational diabetes mellitus. Moreover, the underlying mechanisms of PPARs in the control of these processes have been discussed as well. Finally, this review’s purposes are to provide more knowledge about the role of PPARs in normal and disturbed pregnancy with trophoblast, so as to find PPAR ligands as a potential therapeutic target in the treatment and prevention of adverse pregnancy outcomes.
Immunity as Cornerstone of Non-Alcoholic Fatty Liver Disease: The Contribution of Oxidative Stress in the Disease Progression
Authors: Dallio Marcello , Sangineto Moris , Romeo Mario , Villani Rosanna , Romano Antonino Davide , Loguercio Carmelina , Serviddio Gaetano , Federico Alessandro
Published: January 2021

Non-alcoholic fatty liver disease (NAFLD) is considered the hepatic manifestation of metabolic syndrome and has become the major cause of chronic liver disease, especially in western countries. NAFLD encompasses a wide spectrum of hepatic histological alterations, from simple steatosis to steatohepatitis and cirrhosis with a potential development of hepatocellular carcinoma. Non-alcoholic steatohepatitis (NASH) is characterized by lobular inflammation and fibrosis. Several studies reported that insulin resistance, redox unbalance, inflammation, and lipid metabolism dysregulation are involved in NAFLD progression. However, the mechanisms beyond the evolution of simple steatosis to NASH are not clearly understood yet. Recent findings suggest that different oxidized products, such as lipids, cholesterol, aldehydes and other macromolecules could drive the inflammation onset. On the other hand, new evidence indicates innate and adaptive immunity activation as the driving force in establishing liver inflammation and fibrosis. In this review, we discuss how immunity, triggered by oxidative products and promoting in turn oxidative stress in a vicious cycle, fuels NAFLD progression. Furthermore, we explored the emerging importance of immune cell metabolism in determining inflammation, describing the potential application of trained immune discoveries in the NASH pathological context.
Membrane Sterol Composition in Arabidopsis thaliana Affects Root Elongation via Auxin Biosynthesis
Authors: Wang Meng , Li Panpan , Ma Yao , Nie Xiang , Grebe Markus , Men Shuzhen
Published: January 2021

Plant membrane sterol composition has been reported to affect growth and gravitropism via polar auxin transport and auxin signaling. However, as to whether sterols influence auxin biosynthesis has received little attention. Here, by using the sterol biosynthesis mutant cyclopropylsterol isomerase1-1 (cpi1-1) and sterol application, we reveal that cycloeucalenol, a CPI1 substrate, and sitosterol, an end-product of sterol biosynthesis, antagonistically affect auxin biosynthesis. The short root phenotype of cpi1-1 was associated with a markedly enhanced auxin response in the root tip. Both were neither suppressed by mutations in polar auxin transport (PAT) proteins nor by treatment with a PAT inhibitor and responded to an auxin signaling inhibitor. However, expression of several auxin biosynthesis genes TRYPTOPHAN AMINOTRANSFERASE OF ARABIDOPSIS1 (TAA1) was upregulated in cpi1-1. Functionally, TAA1 mutation reduced the auxin response in cpi1-1 and partially rescued its short root phenotype. In support of this genetic evidence, application of cycloeucalenol upregulated expression of the auxin responsive reporter DR5:GUS (β-glucuronidase) and of several auxin biosynthesis genes, while sitosterol repressed their expression. Hence, our combined genetic, pharmacological, and sterol application studies reveal a hitherto unexplored sterol-dependent modulation of auxin biosynthesis during Arabidopsis root elongation.
Impairment of IGF-1 Signaling and Antioxidant Response Are Associated with Radiation Sensitivity and Mortality
Authors: Y. Aghdam Saeed , Kenchegowda Doreswamy , Holmes-Hampton Gregory P. , Moroni Maria , P. Ghosh Sanchita
Published: January 2021

Following exposure to high doses of ionizing radiation, diverse strains of vertebrate species will manifest varying levels of radiation sensitivity. To understand the inter-strain cellular and molecular mechanisms of radiation sensitivity, two mouse strains with varying radiosensitivity (C3H/HeN, and CD2F1), were exposed to total body irradiation (TBI). Since Insulin-like Growth Factor-1 (IGF-1) signaling pathway is associated with radiosensitivity, we investigated the link between systemic or tissue-specific IGF-1 signaling and radiosensitivity. Adult male C3H/HeN and CD2F1 mice were irradiated using gamma photons at Lethal Dose-70/30 (LD70/30), 7.8 and 9.35 Gy doses, respectively. Those mice that survived up to 30 days post-irradiation, were termed the survivors. Mice that were euthanized prior to 30 days post-irradiation due to deteriorated health were termed decedents. The analysis of non-irradiated and irradiated survivor and decedent mice showed that inter-strain radiosensitivity and post-irradiation survival outcomes are associated with activation status of tissue and systemic IGF-1 signaling, nuclear factor erythroid 2–related factor 2 (Nrf2) activation, and the gene expression profile of cardiac mitochondrial energy metabolism pathways. Our findings link radiosensitivity with dysregulation of IGF-1 signaling, and highlight the role of antioxidant gene response and mitochondrial function in radiation sensitivity.
Scedosporium and Lomentospora Infections: Contemporary Microbiological Tools for the Diagnosis of Invasive Disease
Authors: Chen Sharon C.-A. , Halliday Catriona L. , Hoenigl Martin , Cornely Oliver A. , Meyer Wieland
Published: January 2021

Scedosporium/Lomentospora fungi are increasingly recognized pathogens. As these fungi are resistant to many antifungal agents, early diagnosis is essential for initiating targeted drug therapy. Here, we review the microbiological tools for the detection and diagnosis of invasive scedosporiosis and lomentosporiosis. Of over 10 species, Lomentospora prolificans, Scedosporium apiospermum, S. boydii and S. aurantiacum cause the majority of infections. Definitive diagnosis relies on one or more of visualization, isolation or detection of the fungus from clinical specimens by microscopy techniques, culture and molecular methods such as panfungal PCR or genus-/species-specific multiplex PCR. For isolation from respiratory tract specimens, selective media have shown improved isolation rates. Species identification is achieved by macroscopic and microscopic examination of colonies, but species should be confirmed by ITS with or without β-tubulin gene sequencing or other molecular methods. Matrix-assisted laser desorption ionization-time of flight mass spectrometry databases are improving but may need supplementation by in-house spectra for species identification. Reference broth microdilution methods is preferred for antifungal susceptibility testing. Next-generation sequencing technologies have good potential for characterization of these pathogens. Diagnosis of Scedosporium/Lomentospora infections relies on multiple approaches encompassing both phenotypic- and molecular-based methods.
Effect of Denture Base Fabrication Technique on Candida albicans Adhesion In Vitro
Authors: Meirowitz Avi , Rahmanov Arkadi , Shlomo Eti , Zelikman Helena , Dolev Eran , Sterer Nir
Published: January 2021

Denture stomatitis is a common manifestation of oral candidiasis affecting some 65% of denture wearers. This condition is initiated by the adherence of Candida albicans to denture base acrylic resin. The present study aimed to test the in vitro effect of traditional and novel fabrication methods on Candida albicans adhesion to denture base samples. Denture based acrylic discs were fabricated using: (i) computerized milling, (ii) 3D printing, (iii) heat curing, and (iv) cold curing. Discs were tested for surface roughness (Ra), hydrophobicity (contact angle), mucin adsorption (Bradford assay), and Candida albicans adhesion. 3D printing significantly increased microbial cell adhesion as compared with heat curing, and computerized milling significantly decreased it. These results were associated with mucin adsorption levels rather than surface roughness. Results suggest that 3D printing may increase the risk for developing denture stomatitis, whereas computerized milling may decrease it as compared with traditional heat curing denture base fabrication.
Quantitative Analysis of Biogenic Amines in Different Cheese Varieties Obtained from the Korean Domestic and Retail Markets
Authors: Kandasamy Sujatha , Yoo Jayeon , Yun Jeonghee , Kang Han Byul , Seol Kuk-Hwan , Ham Jun-Sang
Published: January 2021

To evaluate the safety and risk assessment of cheese consumption in the Republic of Korea, sixty cheese samples purchased from the farmstead and retails markets (imported) were analyzed for their biogenic amine (BA) contents. The BA profiles and quantities of eight amines (tryptamine, 2-phenylethylamine, putrescine, cadaverine, histamine, tyramine, spermidine, and spermine) were determined using high-performance liquid chromatography (HPLC). Spermine was the only amine detectable in all the samples. The BAs of fresh cheeses from both farmstead and retail markets were mostly undetectable, and comparatively at lower levels (<125 mg/kg) than ripened samples. Putrescine was undetectable in all the domestic ripened cheeses. The sum of BA levels in the imported ripened cheeses of Pecorino Romano (1889.75 mg/kg) and Grana Padano (1237.80 mg/kg) exceeds >1000 mg/kg, of which histamine accounts nearly 86 and 77% of the total levels, respectively. The tolerable limits of the potential toxic amines, histamine and tyramine surpassed in four and three imported ripened samples, respectively. Furthermore, the presence of potentiators (putrescine and cadaverine) together in samples even with a lower level of toxic amines alarms the risk in consumption. Therefore, adoption of strict hygienic practices during the entire chain of cheese production, along with obligatory monitoring and regulation of BA in cheeses seems to be mandatory to ensure the safety of the consumers.
Evaluation of the Storage Conditions and Type of Cork Stopper on the Quality of Bottled White Wines
Authors: Díaz-Maroto María Consuelo , López Viñas Manuel , Marchante Lourdes , Alañón María Elena , Díaz-Maroto Ignacio Javier , Pérez-Coello María Soledad
Published: January 2021

The effects of different storage conditions, light exposure, temperature and different commercially available cork stoppers on the phenolic, volatile and sensorial profile of Verdejo wines were studied. Two natural corks of different visual quality and a microgranulated cork stopper were investigated over one year at two different storage conditions. One simulating light exposure and temperature in retail outlets and the other simulating optimal cellar conditions (darkness and 12 °C). The wines stored under commercial conditions showed greater losses of total and free SO2 and higher levels of brown-yellowish tones, related to the oxidation of flavan-3-ols. Although these wines underwent a decrease in the total content of stilbenes, a significant increase in trans-piceid was observed. In addition, these wines suffered important changes in their volatile and sensory profile. Volatile compounds with fruity and floral aromas decreased significantly, while volatile compounds related to aged-type characters, as linalool oxides, vitispirane, TDN or furan derivatives increased. Wines stored in darkness at 12 °C underwent minor changes and their sensory profiles were similar to wine before bottling. The high-quality natural corks and microgranulated corks better preserved the quality of the white wines from a sensory point of view. These results showed that temperature and light exposure conditions (diffuse white LEDs and 24 ± 2 °C) in retail outlets considerably decrease the quality of bottled white wines and, consequently, their shelf life, due to the premature development of aged-type characters.
Effect of Creatine Supplementation on Functional Capacity and Muscle Oxygen Saturation in Patients with Symptomatic Peripheral Arterial Disease: A Pilot Study of a Randomized, Double-Blind Placebo-Controlled Clinical Trial
Authors: Domingues Wagner Jorge Ribeiro , Ritti-Dias Raphael Mendes , Cucato Gabriel Grizzo , Wolosker Nelson , Zerati Antônio Eduardo , Puech-Leão Pedro , Coelho Daniel Boari , Nunhes Pollyana Mayara , Moliterno André Alberto , Avelar Ademar
Published: January 2021

The aim of the study was to verify the effects of creatine (Cr) supplementation on functional capacity (walking capacity; primary outcome) and calf muscle oxygen saturation (StO2) (secondary outcome) in symptomatic peripheral arterial disease (PAD) patients. Twenty-nine patients, of both sexes, were randomized (1:1) in a double-blind manner for administration of placebo (PLA, n = 15) or creatine monohydrate (Cr, n = 14). The supplementation protocol consisted of 20 g/day for 1 week divided into four equal doses (loading phase), followed by single daily doses of 5 g in the subsequent 7 weeks (maintenance phase). Functional capacity (total walking distance) was assessed by the 6 min walk test, and calf muscle StO2 was assessed through near infrared spectroscopy. The measurements were collected before and after loading and after the maintenance phase. The level of significance was p < 0.05. No significant differences were found for function capacity (total walking distance (PLA: pre 389 ± 123 m vs. post loading 413 ± 131 m vs. post maintenance 382 ± 99 m; Cr: pre 373 ± 149 m vs. post loading 390 ± 115 m vs. post maintenance 369 ± 115 m, p = 0.170) and the calf muscle StO2 parameters (p > 0.05). Short- and long-term Cr supplementation does not influence functional capacity and calf muscle StO2 parameters in patients with symptomatic PAD.
COVID-19 Pandemic Is a Call to Search for Alternative Protein Sources as Food and Feed: A Review of Possibilities
Authors: Rzymski Piotr , Kulus Magdalena , Jankowski Maurycy , Dompe Claudia , Bryl Rut , Petitte James N. , Kempisty Bartosz , Mozdziak Paul
Published: January 2021

The coronavirus disease 2019 (COVID-19) pandemic is a global health challenge with substantial adverse effects on the world economy. It is beyond any doubt that it is, again, a call-to-action to minimize the risk of future zoonoses caused by emerging human pathogens. The primary response to contain zoonotic diseases is to call for more strict regulations on wildlife trade and hunting. This is because the origins of coronaviruses such as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), SARS-CoV, Middle East respiratory syndrome coronavirus (MERS-CoV), as well as other viral pathogens (e.g., Ebola, HIV) are traceable to wild animals. Although COVID-19 is not related to livestock animals, the pandemic increased general attention given to zoonotic viral infections—the risk of which can also be associated with livestock. Therefore, this paper discusses the potential transformation of industrial livestock farming and the production of animal products, particularly meat, to decrease the risks for transmission of novel human pathogens. Plant-based diets have a number of advantages, but it is unrealistic to consider them as the only solution offered to the problem. Therefore, a search for alternative protein sources in insect-based foods and cultured meat, important technologies enabling safer meat production. Although both of these strategies offer a number of potential advantages, they are also subject to the number of challenges that are discussed in this paper. Importantly, insect-based foods and cultured meat can provide additional benefits in the context of ecological footprint, an aspect important in light of predicted climate changes. Furthermore, cultured meat can be regarded as ethically superior and supports better food security. There is a need to further support the implementation and expansion of all three approaches discussed in this paper, plant-based diets, insect-based foods, and cultured meat, to decrease the epidemiological risks and ensure a sustainable future. Furthermore, cultured meat also offers a number of additional benefits in the context of environmental impact, ethical issues, and food security.
The Combined Effects of Magnesium Oxide and Inulin on Intestinal Microbiota and Cecal Short-Chain Fatty Acids
Authors: Omori Kanako , Miyakawa Hiroki , Watanabe Aya , Nakayama Yuki , Lyu Yijin , Ichikawa Natsumi , Sasaki Hiroyuki , Shibata Shigenobu
Published: January 2021

Constipation is a common condition that occurs in many people worldwide. While magnesium oxide (MgO) is often used as the first-line drug for chronic constipation in Japan, dietary fiber intake is also recommended. Dietary fiber is fermented by microbiota to produce short-chain fatty acids (SCFAs). SCFAs are involved in regulating systemic physiological functions and circadian rhythm. We examined the effect of combining MgO and the water-soluble dietary fiber, inulin, on cecal SCFA concentration and microbiota in mice. We also examined the MgO administration timing effect on cecal SCFAs. The cecal SCFA concentrations were measured by gas chromatography, and the microbiota was determined using next-generation sequencing. Inulin intake decreased cecal pH and increased cecal SCFA concentrations while combining MgO increased the cecal pH lowered by inulin and decreased the cecal SCFA concentrations elevated by inulin. When inulin and MgO were combined, significant changes in the microbiota composition were observed compared with inulin alone. The MgO effect on the cecal acetic acid concentration was less when administered at ZT12 than at ZT0. In conclusion, this study suggests that MgO affects cecal SCFA and microbiota during inulin feeding, and the effect on acetic acid concentration is time-dependent.
Retinoids in Cutaneous Squamous Cell Carcinoma
Authors: Everts Helen B. , Akuailou Eleonore-Nausica
Published: January 2021

Animal studies as early as the 1920s suggested that vitamin A deficiency leads to squamous cell metaplasia in numerous epithelial tissues including the skin. However, humans usually die from vitamin A deficiency before cancers have time to develop. A recent long-term cohort study found that high dietary vitamin A reduced the risk of cutaneous squamous cell carcinoma (cSCC). cSCC is a form of nonmelanoma skin cancer that primarily occurs from excess exposure to ultraviolet light B (UVB). These cancers are expensive to treat and can lead to metastasis and death. Oral synthetic retinoids prevent the reoccurrence of cSCC, but side effects limit their use in chemoprevention. Several proteins involved in vitamin A metabolism and signaling are altered in cSCC, which may lead to retinoid resistance. The expression of vitamin A metabolism proteins may also have prognostic value. This article reviews what is known about natural and synthetic retinoids and their metabolism in cSCC.
Osteoarthritis In Vitro Models: Applications and Implications in Development of Intra-Articular Drug Delivery Systems
Authors: Salgado Carlota , Jordan Olivier , Allémann Eric
Published: January 2021

Osteoarthritis (OA) is a complex multi-target disease with an unmet medical need for the development of therapies that slow and potentially revert disease progression. Intra-articular (IA) delivery has seen a surge in osteoarthritis research in recent years. As local administration of molecules, this represents a way to circumvent systemic drug delivery struggles. When developing intra-articular formulations, the main goals are a sustained and controlled release of therapeutic drug doses, taking into account carrier choice, drug molecule, and articular joint tissue target. Therefore, the selection of models is critical when developing local administration formulation in terms of accurate outcome assessment, target and off-target effects and relevant translation to in vivo. The current review highlights the applications of OA in vitro models in the development of IA formulation by means of exploring their advantages and disadvantages. In vitro models are essential in studies of OA molecular pathways, understanding drug and target interactions, assessing cytotoxicity of carriers and drug molecules, and predicting in vivo behaviors. However, further understanding of molecular and tissue-specific intricacies of cellular models for 2D and 3D needs improvement to accurately portray in vivo conditions.
Investigation of the Shape-Memory Properties of 3D Printed PLA Structures with Different Infills
Authors: Ehrmann Guido , Ehrmann Andrea
Published: January 2021

Polylactic acid (PLA) belongs to the few thermoplastic polymers that are derived from renewable resources such as corn starch or sugar cane. PLA is often used in 3D printing by fused deposition modeling (FDM) as it is relatively easy to print, does not show warping and can be printed without a closed building chamber. On the other hand, PLA has interesting mechanical properties which are influenced by the printing parameters and geometries. Here we present shape-memory properties of PLA cubes with different infill patterns and percentages, extending the research reported before in a conference paper. We investigate the material response under defined quasi-static load as well as the possibility to restore the original 3D printed shape. The quasi-static flexural properties are linked to the porosity and the infill structure of the samples under investigation as well as to the numbers of closed top layers, examined optically and by simulations. Our results underline the importance of designing the infill patterns carefully to develop samples with desired mechanical properties.
Multi-Temporal Speckle Filtering of Polarimetric P-Band SAR Data over Dense Tropical Forests: Study Case in French Guiana for the BIOMASS Mission
Authors: Gelas Colette , Villard Ludovic , Ferro-Famil Laurent , Polidori Laurent , Koleck Thierry , Daniel Sandrine
Published: January 2021

The purpose of this paper is twofold, considering first the generalization of a multichannel speckle filter in order to handle temporal stacks of polarimetric SLC SAR data, and secondly the development of an ad hoc performance indicator based on the Polarimetric Orientation Angle (POA) in order to better estimate the resulting speckle reduction than the standard Equivalent Number of Looks (ENL) over densely vegetated regions, like tropical forests. Being based on the ability of PolSAR measurements to retrieve ground slopes through dense vegetation, this performance indicator requires the use of low frequencies such as P-band, as well as fully polarimetric data. This study has thereby a particular interest in the context of the upcoming BIOMASS spaceborne mission whose launch is scheduled in 2023, and makes use of data from the TropiSAR airborne campaign initiated in the early stage of the mission developments. Conducted over several test sites of tropical dense forests in French Guiana, this campaign gives us the opportunity herein to exploit P-band temporal stacks with repeated time intervals transposable to BIOMASS in terms of signal decorrelation. The application of the generalized multichannel speckle filter to the Paracou test site dataset reveals the limitations of the standard ENL analytical formula to assess speckle reduction in the case of spatially correlated media like dense forests, and for this purpose the interest of the correlation between POA and azimuthal slopes computed from an independent Digital Surface Model.
The Influence of Camera Calibration on Nearshore Bathymetry Estimation from UAV Videos
Authors: Simarro Gonzalo , Calvete Daniel , Plomaritis Theocharis A. , Moreno-Noguer Francesc , Giannoukakou-Leontsini Ifigeneia , Montes Juan , Durán Ruth
Published: January 2021

Measuring the nearshore bathymetry is critical in coastal management and morphodynamic studies. The recent advent of Unmanned Aerial Vehicles (UAVs), in combination with coastal video monitoring techniques, allows for an alternative and low cost evaluation of the nearshore bathymetry. Camera calibration and stabilization is a critical issue in bathymetry estimation from video systems. This work introduces a new methodology in order to obtain such bathymetries, and it compares the results to echo-sounder ground truth data. The goal is to gain a better understanding on the influence of the camera calibration and stabilization on the inferred bathymetry. The results show how the proposed methodology allows for accurate evaluations of the bathymetry, with overall root mean square errors in the order of 40 cm. It is shown that the intrinsic calibration of the camera, related to the lens distortion, is the most critical aspect. Here, the intrinsic calibration that was obtained directly during the flight yields the best results.
Autonomous Navigation of a Team of Unmanned Surface Vehicles for Intercepting Intruders on a Region Boundary
Authors: Marzoughi Ali , Savkin Andrey V.
Published: January 2021

We study problems of intercepting single and multiple invasive intruders on a boundary of a planar region by employing a team of autonomous unmanned surface vehicles. First, the problem of intercepting a single intruder has been studied and then the proposed strategy has been applied to intercepting multiple intruders on the region boundary. Based on the proposed decentralised motion control algorithm and decision making strategy, each autonomous vehicle intercepts any intruder, which tends to leave the region by detecting the most vulnerable point of the boundary. An efficient and simple mathematical rules based control algorithm for navigating the autonomous vehicles on the boundary of the see region is developed. The proposed algorithm is computationally simple and easily implementable in real life intruder interception applications. In this paper, we obtain necessary and sufficient conditions for the existence of a real-time solution to the considered problem of intruder interception. The effectiveness of the proposed method is confirmed by computer simulations with both single and multiple intruders.
Hybrid Blockchain for IoT—Energy Analysis and Reward Plan
Authors: Hu Jiejun , Reed Martin J. , Al-Naday Mays , Thomos Nikolaos
Published: January 2021

Blockchain technology has brought significant advantages for security and trustworthiness, in particular for Internet of Things (IoT) applications where there are multiple organisations that need to verify data and ensure security of shared smart contracts. Blockchain technology offers security features by means of consensus mechanisms; two key consensus mechanisms are, Proof of Work (PoW) and Practical Byzantine Fault Tolerance (PBFT). While the PoW based mechanism is computationally intensive, due to the puzzle solving, the PBFT consensus mechanism is communication intensive due to the all-to-all messages; thereby, both may result in high energy consumption and, hence, there is a trade-off between the computation and the communication energy costs. In this paper, we propose a hybrid-blockchain (H-chain) framework appropriate for scenarios where multiple organizations exist and where the framework enables private transaction verification and public transaction sharing and audit, according to application needs. In particular, we study the energy consumption of the hybrid consensus mechanisms in H-chain. Moreover, this paper proposes a reward plan to incentivize the blockchain agents so that they make contributions to the H-chain while also considering the energy consumption. While the work is generally applicable to IoT applications, the paper illustrates the framework in a scenario which secures an IoT application connected using a software defined network (SDN). The evaluation results first provide a method to balance the public and private parts of the H-chain deployment according to network conditions, computation capability, verification complexity, among other parameters. The simulation results demonstrate that the reward plan can incentivize the blockchain agents to contribute to the H-chain considering the energy consumption of the hybrid consensus mechanism, this enables the proposed H-chain to achieve optimal social welfare.
Assessing the Security of Campus Networks: The Case of Seven Universities
Authors: Zheng Rui , Ma Hao , Wang Qiuyun , Fu Jianming , Jiang Zhengwei
Published: January 2021

The network security situation of campus networks on CERNET (China Education and Research Network) has received great concern. However, most network managers have no complete picture of the network security because of its special management and the rapid growth of network assets. In this investigation, the security of campus networks belonging to seven universities in Wuhan was investigated. A tool called “WebHunt” was designed for campus networks, and with its help, the network security risks were found. Differently from existing tools for network probing, WebHunt can adopt the network scale and special rules of the campus network. According to the characteristics of campus websites, a series of functions were integrated into WebHunt, including reverse resolution of domain names, active network detection and fingerprint identification for software assets. Besides, WebHunt builds its vulnerability intelligence database with a knowledge graph structure and locates the vulnerabilities through matching knowledge graph information. Security assessments of seven universities presents WebHunt’s applicability for campus networks. Besides, it also shows that many security risks are concealed in campus networks, such as non-compliance IP addresses and domain names, system vulnerabilities and so on. The security reports containing risks have been sent to the relevant universities, and positive feedback was received.
A Low-Resources TDC for Multi-Channel Direct ToF Readout Based on a 28-nm FPGA
Authors: Parsakordasiabi Mojtaba , Vornicu Ion , Rodríguez-Vázquez Ángel , Carmona-Galán Ricardo
Published: January 2021

In this paper, we present a proposed field programmable gate array (FPGA)-based time-to-digital converter (TDC) architecture to achieve high performance with low usage of resources. This TDC can be employed for multi-channel direct Time-of-Flight (ToF) applications. The proposed architecture consists of a synchronizing input stage, a tuned tapped delay line (TDL), a combinatory encoder of ones and zeros counters, and an online calibration stage. The experimental results of the TDC in an Artix-7 FPGA show a differential non-linearity (DNL) in the range of [−0.953, 1.185] LSB, and an integral non-linearity (INL) within [−2.750, 1.238] LSB. The measured LSB size and precision are 22.2 ps and 26.04 ps, respectively. Moreover, the proposed architecture requires low FPGA resources.
Narcissistic Personality Traits and Sexual Satisfaction in Men: The Role of Sexual Self-Esteem
Authors: Anzani Annalisa , Di Sarno Marco , Di Pierro Rossella , Prunas Antonio
Published: January 2021

Research on pathological narcissistic traits and sexuality are mainly focused on the dysfunctional aspects of sexuality. The present study aims to explore the relationship between narcissistic traits and sexual satisfaction in men, testing whether sexual self-esteem mediates this association. Participants included 212 men who completed measures of grandiose and vulnerable narcissistic traits, sexual self-esteem, and sexual satisfaction. Results highlight how vulnerable narcissistic traits are negatively associated with sexual satisfaction. This association is fully mediated by sexual self-esteem. On the contrary, grandiose narcissistic traits are not directly associated with sexual satisfaction, but with sexual self-esteem only, which explains the indirect effect of grandiose traits on sexual satisfaction. In conclusion, sexual self-esteem in personality configurations with high pathological narcissistic traits accounts for the relationship between narcissistic traits and sexual satisfaction.
Entrepreneurial Behavior of SMEs and Characteristics of the Managers of Northwest Mexico
Authors: Ochoa Jiménez Sergio , García García Alma Rocío , Leyva Osuna Beatriz Alicia , Valdez del Río Sacnicté
Published: January 2021

The objective of this study is to determine the difference in the entrepreneurial behavior of companies based on the demographic characteristics of their manager or leader. To comply with the above, a quantitative, transversal, and non-experimental research study was carried out, which consisted in applying an instrument to 262 managers of small and medium enterprises (SMEs) in a northern city in Mexico. The collected information was analyzed in the software SPSS, version 26, with statistical testing by the Mann-Whitney and Kruskal-Wallis tests. The main findings show that these companies have differences in their entrepreneurial behavior based on the age and educational level of their managers, while gender and seniority at work are not differentiating elements in relation to the above. This research generates different possibilities of studies to be carried out in large companies from other sectors, and suggests the inclusion of behavioral characteristics as study variables.
Sustainability Assessment of Electricity Generation in Niger Using a Weighted Multi-Criteria Decision Approach
Authors: Bhandari Ramchandra , Arce Benjamin Eduardo , Sessa Vittorio , Adamou Rabani
Published: January 2021

The majority of Niger’s population faces a widespread lack of access to electricity. Although the country lies in the Sahara belt, exploitation of solar energy is so far minimal. Due to ongoing fossil fuel exploration in the country, this fuel might dominate the future electricity supply. Today, Niger imports the most of its electricity from Nigeria. There is a need to expand electricity generation and supply infrastructures in Niger. When doing so, it is important to choose a proper set of electricity generation resource/technology that fulfils sustainability criteria. Thus, the objective of this work is to analyze a methodology in order to assess different energy technologies for Niger. A multi-criteria decision approach was selected to assess the most accessible energy system for the country. For this purpose, indicators were developed and weighted for ranking electricity generation options. Altogether 40 indicators are selected under six dimensions (availability, risk, technology, economics, environment and social) to assess eight different alternatives, considering the aggregated results and corresponding scores under each dimension. A merit list of technology and resources for electricity generation presented in this work could support the stakeholders in their decision-making for further projects implementation in the country.
An Evaluation on Wind Energy Potential Using Multi-Objective Optimization Based Non-Dominated Sorting Genetic Algorithm III
Authors: Subramanian Senthilkumar , Sankaralingam Chandramohan , Elavarasan Rajvikram Madurai , Vijayaraghavan Raghavendra Rajan , Raju Kannadasan , Mihet-Popa Lucian
Published: January 2021

Wind energy is an abundant renewable energy resource that has been extensively used worldwide in recent years. The present work proposes a new Multi-Objective Optimization (MOO) based genetic algorithm (GA) model for a wind energy system. The proposed algorithm consists of non-dominated sorting which focuses to maximize the power extraction of the wind turbine, minimize the cost of generating energy, and the lifetime of the battery. Additionally, the performance characteristics of the wind turbine and battery energy storage system (BESS) are analyzed specifically torque, current, voltage, state of charge (SOC), and internal resistance. The complete analysis is carried out in the MATLAB/Simulink platform. The simulated results are compared with existing optimization techniques such as single-objective, multi-objective, and non-dominating sorting GA II (Genetic Algorithm-II). From the observed results, the non-dominated sorting genetic algorithm (NSGA III) optimization algorithm offers superior performance notably higher turbine power output with higher torque rate, lower speed variation, reduced energy cost, and lesser degradation rate of the battery. This result attested to the fact that the proposed optimization tool can extract a higher rate of power from a self-excited induction generator (SEIG) when compared with a conventional optimization tool.
Evaluation of Mechanical Characteristics of Cement Mortar with Fine Recycled Concrete Aggregates (FRCA)
Authors: Martínez-García Rebeca , Rojas María Isabel Sánchez de , Pozo Julia Mª. Morán-del , Fraile-Fernández Fernando J. , Juan-Valdés Andrés
Published: January 2021

One of the growing demands in concrete manufacture is the availability of natural fine aggregates, which account for 35% to 45% of the total concrete. An alternative method of disposal of fine recycled concrete aggregates (FRCA) generated from demolition and construction waste (C&DW) is their usage in mortar and the development of recycled mortar. The main aim of this research work is to evaluate the viability of incorporating FRCA from urban C&DW for the manufacture of cement-based mortars. Simple processing techniques like washing and sieving are adopted to improve the FRCA quality. Physical and chemical characterization of ingredients is carried out. In total four mixes of 1:3 (cement: sand) mortar with partial replacement of normalized sand with FRCA (0%, 25%, 50%, and 100%) are evaluated for mechanical properties. Water to cement ratio for all four mortar mixes are determined by fixed consistency. Mechanical and physical properties like density, compressive strength, and flexural strength are studied for various curing periods, and the result is that the optimum usage of FRCA is 25% based on a 90-day curing period.
Influence of the Shortening of the Winter Fertilization Prohibition Period in Hungary Assessed by Spatial Crop Simulation Analysis
Authors: Koós Sándor , Pirkó Béla , Szatmári Gábor , Csathó Péter , Magyar Marianna , Szabó József , Fodor Nándor , Pásztor László , Laborczi Annamária , Pokovai Klára , Szabó Anita
Published: January 2021

The Nitrates Directive aims (a) to protect water quality across Europe from nitrates originating from agricultural sources that pollute ground and surface water, and (b) to promote good farming practices. One of the most controversial measures of the directive is the winter prohibition period of fertilization, which has been extended by a month in two steps in recent years. According to the regulation, it is forbidden to apply nitrogen fertilization in Hungary between 31st October and 15th February, even though the winter climate is gradually becoming milder. Using the fertilization data of nearly half a million parcels of land in the Hungarian Nitrate Database, a crop model-based spatial analysis was carried out. Our aim was to test if a shift in the prohibition period starting date from 31st October to 30th November caused any differences in the nitrate amount leached at a 90 cm depth. Detailed nitrate inputs and soil and weather databases were coupled with the 4M crop model. The yield, plant nitrogen uptake, and nitrate leaching under five major crops were simulated, covering a considerable portion of arable land. Shifting the prohibition period starting date did not result in significant changes in the nitrate leaching. Further runs of the 4M model with different weather scenarios are needed to decide whether the modification of the prohibition period significantly affects the amount of nitrate leached.