bluuebunny/crossref_metadata_2025 huggingface dataset:
Detection of Aseismic Slip and Poroelastic Reservoir Deformation at the North Brawley Geothermal Field From 2009 to 2019
Authors: Materna Kathryn , Barbour Andrew , Jiang Junle , Eneva Mariana
Published: April 2022

The North Brawley Geothermal Field, located within the Brawley Seismic Zone of Southern California, presents a case study for understanding seismic hazards linked to fluid injection and geothermal energy extraction. An earthquake swarm near the geothermal field in 2012 included two earthquakes with magnitudes greater than 5 and was potentially preceded by a years‐long aseismic slip transient. To better understand ground deformation around the geothermal field, including its evolution with time and its physical mechanisms, we analyze deformation before, during, and after the swarm using ground‐ and satellite‐based geodetic techniques between 2009 and 2019. We integrate observations from GNSS, Sentinel‐1, TerraSAR‐X, UAVSAR, and leveling surveys into a single deformation history. Modeling of this new collection of observations at the North Brawley Geothermal Field provides evidence for 80% more pre‐swarm aseismic slip than previously recognized from 2009 to 2012. During the 2012 Brawley swarm, our geodetic slip inversions closely match the results of seismic waveform inversions from the swarm events. After the 2012 swarm, surface deformation is dominated by poroelastic deformation of a shallow fluid reservoir at <1 km depth rather than fault slip. The deformation history and seismicity catalogs at North Brawley suggest a cessation of fault‐related slip during the ∼7 years after the 2012 earthquake swarm.
Building a Young Mountain Range: Insight Into the Growth of the Greater Caucasus Mountains From Detrital Zircon (U‐Th)/He Thermochronology and 10Be Erosion Rates
Authors: Forte Adam M. , Gutterman Kate R. , van Soest Matthijs C. , Gallagher Kerry
Published: April 2022

The Greater Caucasus (GC) Mountains within the central Arabia‐Eurasia collision zone are an archetypal example of a young collisional orogen. However, the mechanisms driving rock uplift and forming the topography of the range are controversial, with recent provocative suggestions that uplift of the western GC is strongly influenced by an isostatic response to slab detachment, whereas the eastern half has grown through shortening and crustal thickening. Testing this hypothesis is challenging because records of exhumation rates mostly come from the western GC, where slab detachment may have occurred. To address this data gap, we report 623 new, paired zircon U‐Pb and (U‐Th)/He ages from seven different modern river sediments, spanning a ∼400 km long gap in bedrock thermochronometer data. We synthesize these with prior bedrock thermochronometer data, recent catchment averaged 10Be cosmogenic exhumation rates, topographic analyses, structural observations, and plate reconstructions to evaluate the mechanisms growing the GC topography. We find no evidence of major differences in rates, timing of onset of cooling, or total amounts of exhumation across the possible slab edge, inconsistent with previous suggestions of heterogeneous drivers for exhumation along‐strike. Comparison of exhumation across timescales highlight a potential acceleration, but one that appears to suggest a consistent northward shift of the locus of more rapid exhumation. Integration of these new datasets with simple models of orogenic growth suggest that the gross topography of the GC is explainable with traditional models of accretion, thickening, and uplift and does not require any additional slab‐related mechanisms.
Geoscience Education Perspectives on Integrated, Coordinated, Open, Networked (ICON) Science
Authors:   Conceptual Team:, Fortner Sarah K. , Manduca Cathryn A. , Ali Hendratta N. , Saup Casey M. ,   DEIJ Writing Leads:, Nyarko Samuel C. , Othus‐Gault Shannon , Perera Viranga , Tong Vincent C. H. ,   Geoscience Education Practice Writing Leads:, Gold Anne U. , Furman Tanya ,   Geoscience Education Research Writing Leads:, Arthurs Leilani , Mulvey Bridget K. , St. John Kristen ,   DEIJ Writing Team:, Singley Joel G. , Johnson Elijah Thomas , Witter Molly ,   Geoscience Education Practice Writing Team:, Batchelor Rebecca L. , Carter Deron T. , Damas M. Chantale , LeMay Lynsey , Layou Karen M. , Low Russanne , Wang Hui Hui , Olson‐Sawyer Kai , Pallant Amy , Ryker Katherine ,   Geoscience Education Research Writing Team:, Lukes Laura , LaDue Nicole , Ryker Katherine , van der Hoeven Kraft Kaatje J.
Published: April 2022

Practitioners and researchers in geoscience education embrace collaboration applying ICON (Integrated, Coordinated, Open science, and Networked) principles and approaches which have been used to create and share large collections of educational resources, to move forward collective priorities, and to foster peer‐learning among educators. These strategies can also support the advancement of coproduction between geoscientists and diverse communities. For this reason, many authors from the geoscience education community have co‐created three commentaries on the use and future of ICON in geoscience education. We envision that sharing our expertise with ICON practice will be useful to other geoscience communities seeking to strengthen collaboration. Geoscience education brings substantial expertise in social science research and its application to building individual and collective capacity to address earth sustainability and equity issues at local to global scales The geoscience education community has expanded its own ICON capacity through access to and use of shared resources and research findings, enhancing data sharing and publication, and leadership development. We prioritize continued use of ICON principles to develop effective and inclusive communities that increase equity in geoscience education and beyond, support leadership and full participation of systemically non‐dominant groups and enable global discussions and collaborations.
Long non-coding RNA LINC00511 facilitates colon cancer development through regulating microRNA-625-5p to target WEE1
Authors: Qian Xiaowu , Jiang Chun , Zhu Zhengtai , Han Gaohua , Xu Ning , Ye Jun , Wang Ruixing
Published: April 2022

The altered part of long non-coding RNA LINC00511 (LINC00511) is extensively discussed in malignancies. Finitely, the mechanism of LINC00511 in colon cancer (CC) development lacks thorough explorations. Hence, this work is started from the LINC00511-mediated microRNA (miR)-625-5p/WEE1 axis in the CC process. LINC00511, miR-625-5p, and WEE1 levels were tested in CC tissues and cells. Subcellular localization of LINC00511 was clarified. CC cells were transfected with oligonucleotides that altered LINC00511, and miR-625-5p expression to define their performance in CC cell progression. The tumorigenic ability of cells was verified in xenografted tumors. CC tissues and cells highly expressed LINC00511 and WEE1 and lowly expressed miR-625-5p. LINC00511 was mainly localized in the cytoplasm. Deleted LINC00511 or restored miR-625-5p delayed cellular growth in CC. LINC00511 sponged miR-625-5p to target WEE1. Silenced miR-625-5p mitigated the role of depleted LINC00511, while inhibited WEE1 rescued the effect of silenced miR-625-5p on the biological functions of CC cells. It is summarized that down-regulated LINC00511 obstructs tumorigenesis of CC through restoring miR-625-5p and silencing WEE1, consolidating a basal reference for CC-oriented therapy.
Foldamers reveal and validate therapeutic targets associated with toxic α-synuclein self-assembly
Authors: Ahmed Jemil , Fitch Tessa C. , Donnelly Courtney M. , Joseph Johnson A. , Ball Tyler D. , Bassil Mikaela M. , Son Ahyun , Zhang Chen , Ledreux Aurélie , Horowitz Scott , Qin Yan , Paredes Daniel , Kumar Sunil
Published: April 2022

Parkinson’s disease (PD) is a progressive neurodegenerative disorder for which there is no successful prevention or intervention. The pathological hallmark for PD involves the self-assembly of functional Alpha-Synuclein (αS) into non-functional amyloid structures. One of the potential therapeutic interventions against PD is the effective inhibition of αS aggregation. However, the bottleneck towards achieving this goal is the identification of αS domains/sequences that are essential for aggregation. Using a protein mimetic approach, we have identified αS sequences-based targets that are essential for aggregation and will have significant therapeutic implications. An extensive array of in vitro, ex vivo, and in vivo assays is utilized to validate αS sequences and their structural characteristics that are essential for aggregation and propagation of PD phenotypes. The study aids in developing significant mechanistic and therapeutic insights into various facets of αS aggregation, which will pave the way for effective treatments for PD.
EWSR1-ATF1 dependent 3D connectivity regulates oncogenic and differentiation programs in Clear Cell Sarcoma
Authors: Möller Emely , Praz Viviane , Rajendran Sanalkumar , Dong Rui , Cauderay Alexandra , Xing Yu-Hang , Lee Lukuo , Fusco Carlo , Broye Liliane C. , Cironi Luisa , Iyer Sowmya , Rengarajan Shruthi , Awad Mary E. , Naigles Beverly , Letovanec Igor , Ormas Nicola , Finzi Giovanna , La Rosa Stefano , Sessa Fausto , Chebib Ivan , Petur Nielsen G. , Digklia Antonia , Spentzos Dimitrios , Cote Gregory M. , Choy Edwin , Aryee Martin , Stamenkovic Ivan , Boulay Gaylor , Rivera Miguel N. , Riggi Nicolò
Published: April 2022

Oncogenic fusion proteins generated by chromosomal translocations play major roles in cancer. Among them, fusions between EWSR1 and transcription factors generate oncogenes with powerful chromatin regulatory activities, capable of establishing complex gene expression programs in permissive precursor cells. Here we define the epigenetic and 3D connectivity landscape of Clear Cell Sarcoma, an aggressive cancer driven by the EWSR1-ATF1 fusion gene. We find that EWSR1-ATF1 displays a distinct DNA binding pattern that requires the EWSR1 domain and promotes ATF1 retargeting to new distal sites, leading to chromatin activation and the establishment of a 3D network that controls oncogenic and differentiation signatures observed in primary CCS tumors. Conversely, EWSR1-ATF1 depletion results in a marked reconfiguration of 3D connectivity, including the emergence of regulatory circuits that promote neural crest-related developmental programs. Taken together, our study elucidates the epigenetic mechanisms utilized by EWSR1-ATF1 to establish regulatory networks in CCS, and points to precursor cells in the neural crest lineage as candidate cells of origin for these tumors.
Leveraging omic features with F3UTER enables identification of unannotated 3’UTRs for synaptic genes
Authors: Sethi Siddharth , Zhang David , Guelfi Sebastian , Chen Zhongbo , Garcia-Ruiz Sonia , Olagbaju Emmanuel O. , Ryten Mina , Saini Harpreet , Botia Juan A.
Published: April 2022

There is growing evidence for the importance of 3’ untranslated region (3’UTR) dependent regulatory processes. However, our current human 3’UTR catalogue is incomplete. Here, we develop a machine learning-based framework, leveraging both genomic and tissue-specific transcriptomic features to predict previously unannotated 3’UTRs. We identify unannotated 3’UTRs associated with 1,563 genes across 39 human tissues, with the greatest abundance found in the brain. These unannotated 3’UTRs are significantly enriched for RNA binding protein (RBP) motifs and exhibit high human lineage-specificity. We find that brain-specific unannotated 3’UTRs are enriched for the binding motifs of important neuronal RBPs such as TARDBP and RBFOX1, and their associated genes are involved in synaptic function. Our data is shared through an online resource F3UTER (https://astx.shinyapps.io/F3UTER/). Overall, our data improves 3’UTR annotation and provides additional insights into the mRNA-RBP interactome in the human brain, with implications for our understanding of neurological and neurodevelopmental diseases.
Machine learning predictions of irradiation embrittlement in reactor pressure vessel steels
Authors: Liu Yu-chen , Wu Henry , Mayeshiba Tam , Afflerbach Benjamin , Jacobs Ryan , Perry Josh , George Jerit , Cordell Josh , Xia Jinyu , Yuan Hao , Lorenson Aren , Wu Haotian , Parker Matthew , Doshi Fenil , Politowicz Alexander , Xiao Linda , Morgan Dane , Wells Peter , Almirall Nathan , Yamamoto Takuya , Odette G. Robert
Published: April 2022

Irradiation increases the yield stress and embrittles light water reactor (LWR) pressure vessel steels. In this study, we demonstrate some of the potential benefits and risks of using machine learning models to predict irradiation hardening extrapolated to low flux, high fluence, extended life conditions. The machine learning training data included the Irradiation Variable for lower flux irradiations up to an intermediate fluence, plus the Belgian Reactor 2 and Advanced Test Reactor 1 for very high flux irradiations, up to very high fluence. Notably, the machine learning model predictions for the high fluence, intermediate flux Advanced Test Reactor 2 irradiations are superior to extrapolations of existing hardening models. The successful extrapolations showed that machine learning models are capable of capturing key intermediate flux effects at high fluence. Similar approaches, applied to expanded databases, could be used to predict hardening in LWRs under life-extension conditions.
A soft and stretchable electronics using laser-induced graphene on polyimide/PDMS composite substrate
Authors: Wang Hao , Zhao Zifen , Liu Panpan , Guo Xiaogang
Published: April 2022

The one-step fabricated laser-induced graphene (LIG) has the advantages of low cost, patterning of various desired geometries, and high sensitivity. However, the robustness of substrates imposes certain constraints on their applications in stretchable devices. In this paper, the substrate composed of polydimethylsiloxane (PDMS) and polyimide (PI) particles is proposed to serve as the platform to manufacture LIG. Ascribing to the inherent soft and stretchable attributes of the PI/PDMS composite substrate, the LIG-based sensors can fit complex 3D configurations or bear a mechanical tension over 15%. Notably, the fluence of the laser is experimentally and theoretically determined as the only principle to characterize the formation of conductive LIG on PI/PDMS composite greatly facilitating the selection of the allowable laser scanning parameters to form the desired LIG-based devices. Three demonstrations are conducted to highlight the superiority and the potential of this soft and stretchable LIG-based system in wearable electronics and soft robots.
Immunomodulatory activity of a water-soluble polysaccharide extracted from mussel on cyclophosphamide-induced immunosuppressive mice models
Authors: Xiang Xingwei , Wang Rui , Chen Lin , Chen Yufeng , Zheng Bin , Deng Shanggui , Liu Shulai , Sun Peilong , Shen Guoxin
Published: April 2022

This study aimed to investigate the protective effect of mussel polysaccharide (MP) on cyclophosphamide (Cy)-induced intestinal mucosal immunosuppression and microbial dysbiosis in mice. MP was shown to stimulate secretion of cytokines (SIgA, IL-2, IF-γ, IL-4, IL-10) and production of transcription factors (occludin, claudin-1, ZO-1, mucin-2, IL-2, IF-γ, IL-4, IL-10). Key proteins (p-IκB-α,p-p65) of the NF-κB pathway were upregulated after MP administration. SCFAs levels, which were decreased after the Cy treatment, were improved after treatment with MP. Furthermore, 16 S rRNA sequencing data of fecal samples revealed, through α-diversity and β-diversity analysis, that MP improved microbial community diversity and modulate the overall composition of gut microbiota. Taxonomic composition analysis showed that MP increased the abundance of probiotics species (Lactobacillus) and decreased the proportion of pathogenic species (Desulfovibrio). These findings suggested that MP has a potential immunomodulatory activity on the immunosuppressive mice.
Real-time segmentation method of billet infrared image based on multi-scale feature fusion
Authors: Zhang Lixin , Nan Qingrong , Bian Shengqin , Liu Tao , Xu Zhengguang
Published: April 2022

Obtaining the surface temperature of billets in heating furnaces has been a hot research in metallurgical industry applications. In order to accurately identify the billet location in infrared images and thus obtain the surface temperature of billets, this paper proposes a real-time segmentation network model based on multi-scale feature fusion to solve the problems of low resolution, low accuracy and slow detection speed of infrared images of traditional target image detection methods. In our method, a dataset with billet infrared images as the experimental object is firstly established, and the proposed network structure adopts multi-scale feature fusion to enhance the information interaction between feature maps at all levels and reduce the information loss during up-sampling by a dense up-sampling strategy. Meanwhile, a lightweight backbone network and deep separable convolution are used to reduce the number of network parameters and speed up the network inference, finally realizing real-time and accurate segmentation of the infrared images of blanks. The highest accuracy of the model in this paper reaches 94.89$$\%$$%. Meanwhile, an inference speed of 80fps is achieved on GTX2080Ti. Compared with the existing mainstream methods, the method in this paper can better meet the real-time and accuracy requirements of industrial production.
Identifying key genes in milk fat metabolism by weighted gene co-expression network analysis
Authors: Mu Tong , Hu Honghong , Ma Yanfen , Wen Huiyu , Yang Chaoyun , Feng Xiaofang , Wen Wan , Zhang Juan , Gu Yaling
Published: April 2022

Milk fat is the most important and energy-rich substance in milk, and its content and composition are important reference elements in the evaluation of milk quality. However, the current identification of valuable candidate genes affecting milk fat is limited. IlluminaPE150 was used to sequence bovine mammary epithelial cells (BMECs) with high and low milk fat rates (MFP), the weighted gene co-expression network (WGCNA) was used to analyze mRNA expression profile data in this study. As a result, a total of 10,310 genes were used to construct WGCNA, and the genes were classified into 18 modules. Among them, violet (r = 0.74), yellow (r = 0.75) and darkolivegreen (r =  − 0.79) modules were significantly associated with MFP, and 39, 181, 75 hub genes were identified, respectively. Combining enrichment analysis and differential genes (DEs), we screened five key candidate DEs related to lipid metabolism, namely PI4K2A, SLC16A1, ATP8A2, VEGFD and ID1, respectively. Relative to the small intestine, liver, kidney, heart, ovary and uterus, the gene expression of PI4K2A is the highest in mammary gland, and is significantly enriched in GO terms and pathways related to milk fat metabolism, such as monocarboxylic acid transport, phospholipid transport, phosphatidylinositol signaling system, inositol phosphate metabolism and MAPK signaling pathway. This study uses WGCNA to form an overall view of MFP, providing a theoretical basis for identifying potential pathways and hub genes that may be involved in milk fat synthesis.
The nano- and meso-scale structure of amorphous calcium carbonate
Authors: Clark Simon M. , Colas Bruno , Jacob Dorrit E. , Neuefeind Joerg C. , Wang Hsiu-Wen , Page Katherine L. , Soper Alan K. , Schodder Philipp I. , Duchstein Patrick , Zubiri Benjamin Apeleo , Yokosawa Tadahiro , Pipich Vitaliy , Zahn Dirk , Spiecker Erdmann , Wolf Stephan E.
Published: April 2022

Understanding the underlying processes of biomineralization is crucial to a range of disciplines allowing us to quantify the effects of climate change on marine organisms, decipher the details of paleoclimate records and advance the development of biomimetic materials. Many biological minerals form via intermediate amorphous phases, which are hard to characterize due to their transient nature and a lack of long-range order. Here, using Monte Carlo simulations constrained by X-ray and neutron scattering data together with model building, we demonstrate a method for determining the structure of these intermediates with a study of amorphous calcium carbonate (ACC) which is a precursor in the bio-formation of crystalline calcium carbonates. We find that ACC consists of highly ordered anhydrous nano-domains of approx. 2 nm that can be described as nanocrystalline. These nano-domains are held together by an interstitial net-like matrix of water molecules which generate, on the mesoscale, a heterogeneous and gel-like structure of ACC. We probed the structural stability and dynamics of our model on the nanosecond timescale by molecular dynamics simulations. These simulations revealed a gel-like and glassy nature of ACC due to the water molecules and carbonate ions in the interstitial matrix featuring pronounced orientational and translational flexibility. This allows for viscous mobility with diffusion constants four to five orders of magnitude lower than those observed in solutions. Small and ultra-small angle neutron scattering indicates a hierarchically-ordered organization of ACC across length scales that allow us, based on our nano-domain model, to build a comprehensive picture of ACC formation by cluster assembly from solution. This contribution provides a new atomic-scale understanding of ACC and provides a framework for the general exploration of biomineralization and biomimetic processes.
Bone marrow transplantation induces changes in the gut microbiota that chronically increase the cytokine response pattern of splenocytes
Authors: Katiraei Saeed , van Diepen Janna A. , Tavares Luciana P. , Hoving Lisa R. , Pronk Amanda , Verschueren Ineke , Rensen Patrick C. N. , Zwaginga Jaap Jan , Kostidis Sarantos , Giera Martin , Teixera Mauro , van Dijk Ko Willems , Netea Mihai G. , Berbée Jimmy F. P. , van Harmelen Vanessa
Published: April 2022

Bone marrow transplantation (BMT) involves conditioning regimens which acutely induce side effects, including systemic inflammation, intestinal damage and shifts in the gut microbial composition, some of which may persist chronically. As the gut microbiota affect systemic immune responses, we aimed to investigate whether, post-BMT, the peripheral immune system is modulated as a direct consequence of alterations in the gut microbiota. We show that 24 weeks post-BMT, splenocytes but not peritoneal macrophages display increased cytokine response patterns upon ex-vivo stimulation with various pathogens as compared to untreated controls. The pattern of BMT-induced cytokine responses was transferred to splenocytes, and not to peritoneal macrophages, of healthy controls via co-housing and transferred to germfree mice via transplantation of cecum content. Thus, BMT induces changes in gut microbiota that in their turn increase cytokine responsiveness of splenocytes. Thus, BMT establishes a dominant microbiota that attenuates normalization of the immune-response.
Establishment and a comparative transcriptomic analysis of a male-specific cell line from the African malaria mosquito Anopheles gambiae
Authors: Krzywinska Elzbieta , Ferretti Luca , Krzywinski Jaroslaw
Published: April 2022

Cell lines allow studying various biological processes that may not be easily tractable in whole organisms. Here, we have established the first male-specific cell line from the African malaria mosquito, Anopheles gambiae. The cells, named AgMM and derived from the sex-sorted neonate larvae, were able to undergo spontaneous contractions for a number of passages following establishment, indicating their myoblast origin. Comparison of their transcriptome to the transcriptome of an A. gambiae-derived Sua5.1 hemocyte cells revealed distinguishing molecular signatures of each cell line, including numerous muscle-related genes that were highly and uniquely expressed in the AgMM cells. Moreover, the AgMM cells express the primary sex determiner gene Yob and support male sex determination and dosage compensation pathways. Therefore, the AgMM cell line represents a valuable tool for molecular and biochemical in vitro studies of these male-specific processes. In a broader context, a rich transcriptomic data set generated in this study contributes to a better understanding of transcribed regions of the A. gambiae genome and sheds light on the biology of both cell types, facilitating their anticipated use for various cell-based assays.
A perspective of scale differences for studying the green total factor productivity of Chinese laying hens
Authors: Zhong Shen , Chen Xi , Li Junwei , Jiang Shubo
Published: April 2022

In people’s daily life, egg is one of the main animal protein foods, which will produce different emissions on its breeding procedure. Therefore, in order to promote the development of China’s layer industry, it is necessary to reduce pollutant emissions by improving efficiency. This paper uses Minimum distance to weak efficient frontier-Metafrontier Malmquist Luenberger (MinDW-MML) index model, by considering environmental factors and scale heterogeneity, to explore the evolution characteristics of laying hens breeding green total factor productivity (LHG) in China based on the data of 24 major laying provinces (municipalities) from 2004 to 2018. The results show that: (1) From 2004 to 2018, medium-scale LHG in China is the highest, the small-scale is the second, and the large-scale is the lowest. In the light of regional distribution, the western region is the highest, followed by the central region, and the eastern region is the lowest. (2) From 2004 to 2018, the overall China’s LHG showed a positive growth, and the decomposition indicators were characterized by decreased efficiency and technological progress. In general, the layer industry is vulnerable and easily affected by external factors. (3) Results from common frontiers and group frontiers exist some differences. The LHG under the common frontier is lower than the LHG under the group frontier. Finally, according to the above empirical results, this paper puts forward policy suggestions to improve LHG and environmental protection suggestions for laying hens.
Association between intraoperative end-tidal carbon dioxide and postoperative nausea and vomiting in gynecologic laparoscopic surgery
Authors: Dong Li , Takeda Chikashi , Yamazaki Hajime , Hamada Miho , Hirotsu Akiko , Yamamoto Yosuke , Mizota Toshiyuki
Published: April 2022

Gynecologic laparoscopic surgery has a high incidence of postoperative nausea and vomiting (PONV). Studies suggest that low intraoperative end-tidal carbon dioxide (EtCO2) is associated with an increased incidence of PONV, but the results have not been consistent among studies. This study investigated the association between intraoperative EtCO2 and PONV in patients undergoing gynecologic laparoscopic surgeries under general anesthesia. This retrospective cohort study involved patients who underwent gynecologic laparoscopic surgeries under general anesthesia at Kyoto University Hospital. We defined low EtCO2 as a mean EtCO2 of < 35 mmHg. Multivariable modified Poisson regression analysis examined the association between low EtCO2 and PONV during postoperative two days and the postoperative length of hospital stay (PLOS). Of the 739 patients, 120 (16%) had low EtCO2, and 430 (58%) developed PONV during postoperative two days. There was no substantial association between low EtCO2 and increased incidence of PONV (adjusted risk ratio: 0.96; 95% confidence interval [CI] 0.80–1.14; p = 0.658). Furthermore, there was no substantial association between low EtCO2 and prolonged PLOS (adjusted difference in PLOS: 0.13; 95% CI − 1.00 to 1.28; p = 0.816). Intraoperative low EtCO2, specifically a mean intraoperative EtCO2 below 35 mmHg, was not substantially associated with either increased incidence of PONV or prolonged PLOS.
Enhanced biomedical applicability of ZrO2–SiO2 ceramic composites in 3D printed bone scaffolds
Authors: Chang Chih-Hao , Lin Chih-Yang , Chang Chih-Hung , Liu Fwu-Hsing , Huang Yu-Tzu , Liao Yunn-Shiuan
Published: April 2022

Zirconia (ZrO2) has been widely used in clinical applications, such as bone and dental implantation, because of its favorable mechanical properties and resistance to fracture. However, the poor cell affinity of ZrO2 for bone regeneration and tissue binding, as well as its shrinkage due to crystal phase transformation during heat treatment, limits its clinical use and processing plasticity. This study aims to investigate an appropriate ZrO2–SiO2 composite recipe for ceramic 3D printing processes that can strike a balance between the mechanical properties and cell affinity needed in clinical applications. Specimens with different ZrO2–SiO2 composite recipes were fabricated by a selective laser gelling method and sintered at temperatures ranging from 900 to 1500 °C. The S5Z5 composite, which consists of 50 wt% ZrO2, 35 wt% SiO2 and 15 wt% SiO2 sol, showed an appropriate compressive strength and bending strength of 82.56 MPa and 55.98 MPa, respectively, at a sintering temperature of 1300 °C. The shrinkage rate of the S5Z5 composite was approximately 5% when the sintering temperature was increased from 900 to 1500 °C. All composites exhibited no cytotoxicity after 144 h of MG63 cell incubation, and the S5Z5 composite exhibited the most obvious cell affinity among the composite recipes. From these results, compared with other composites, the S5Z5 composite was shown to possess mechanical properties and a cell affinity more comparable to those of natural human bone.
Group-velocity-locked vector solitons and dissipative solitons in a single fiber laser with net-anomalous dispersion
Authors: Xu Shutao , Turnali Ahmet , Sander Michelle Y.
Published: April 2022

Laser cavities which can generate different types of ultrashort pulses are attractive for practical applications and the study of pulse dynamics. Here, we report the first experimental observation of both conventional solitons (CS) and dissipative solitons (DS) generated from a single all-fiber laser with net-anomalous dispersion. A birefringence-related intracavity Lyot filter with an adjustable extinction ratio enables the switching between the two types of ultrashort pulses. Depending on the polarization controller settings and the pump power, either chirp-free CS with a pulse energy of 406 pJ and a spectral bandwidth of 5.1 nm or up-chirped DS with a pulse energy of 5.1 nJ and an optical bandwidth of 9.6 nm can be generated. Similar polarization features are observed when the laser switches between different soliton operations as both CS and DS are group-velocity-locked vector solitons. Our work paves a novel way to generate dissipative solitons with a relatively high pulse energy (one order of magnitude larger than for CS) and a large chirp directly from an all-fiber net-anomalous-dispersion cavity through birefringent filter management.
Impact of disabled circadian clock on yellow fever mosquito Aedes aegypti fitness and behaviors
Authors: Shetty Vinaya , Meyers Jacob I. , Zhang Ying , Merlin Christine , Slotman Michel A.
Published: April 2022

Like other insects, Aedes aegypti displays strong daily patterns in host seeking and mating. Much of these behaviors are believed to be under the control of a circadian clock, an endogenous timekeeping mechanism relying on transcriptional/translational negative feedback loops that drive rhythmic physiology and behavior. To examine the connection between the circadian clock and various Ae. aegypti behaviors, we knocked out the core clock gene cycle using CRISPR/Cas9. We found that the rhythmic pattern and intensity of mRNA expression of seven circadian genes, including AeCyc−/−, were altered across the day/night cycle as well as in constant darkness conditions. We further show that the mutant CYC protein is incapable of forming a dimer with CLK to stimulate per expression and that the endogenous clock is disabled in AeCyc−/− mosquitoes. AeCyc−/− do not display the bimodal locomotor activity pattern of wild type, have a significantly reduced response to host odor, reduced egg hatching rates, delayed embryonic development and reduced adult survival and mating success. Surprisingly however, the propensity to blood feed in AeCyc−/− females is significantly higher than in wildtype females. Together with other recent work on the circadian clock control of key aspects of mosquito biology, our data on how cycle KO affects mosquito behavior and fitness provides a basis for further work into the pathways that connect the mosquito endogenous clock to its vector competence.
Beam width and arm position but not cognitive task affect walking balance in older adults
Authors: da Silva Costa Andréia Abud , Hortobágyi Tibor , Otter Rob den , Sawers Andrew , Moraes Renato
Published: April 2022

Detection of changes in dynamic balance could help identify older adults at fall risk. Walking on a narrow beam with its width, cognitive load, and arm position manipulated could be an alternative to current tests. Therefore, we examined additive and interactive effects of beam width, cognitive task (CT), and arm position on dynamic balance during beam walking in older adults. Twenty older adults (69 ± 4y) walked on 6, 8, and 10-cm wide beams (2-cm high, 4-m-long), with and without CT, with three arm positions (free, crossed, akimbo). We determined cognitive errors, distance walked, step speed, root mean square (RMS) of center of mass (COM) displacement and trunk acceleration in the frontal plane. Beam width decrease progressively reduced distance walked and increased trunk acceleration RMS. Step speed decreased on the narrowest beam and with CT. Arm crossing decreased distance walked and step speed. COM displacement RMS and cognitive errors were not affected by any manipulation. In conclusion, distance walked indicated that beam width and arm position, but less so CT, affected dynamic balance, implying that beam walking has the potential to become a test of fall risk. Stability measurements suggested effective trunk adjustments to control COM position and keep dynamic balance during the task.
Endothelial inflammation and neutrophil transmigration are modulated by extracellular matrix composition in an inflammation-on-a-chip model
Authors: Riddle Rebecca B. , Jennbacken Karin , Hansson Kenny M. , Harper Matthew T.
Published: April 2022

Inflammatory diseases are often characterised by excessive neutrophil infiltration from the blood stream to the site of inflammation, which damages healthy tissue and prevents resolution of inflammation. Development of anti-inflammatory drugs is hindered by lack of in vitro and in vivo models which accurately represent the disease microenvironment. In this study, we used the OrganoPlate to develop a humanized 3D in vitro inflammation-on-a-chip model to recapitulate neutrophil transmigration across the endothelium and subsequent migration through the extracellular matrix (ECM). Human umbilical vein endothelial cells formed confluent vessels against collagen I and geltrex mix, a mix of basement membrane extract and collagen I. TNF-α-stimulation of vessels upregulated inflammatory cytokine expression and promoted neutrophil transmigration. Intriguingly, major differences were found depending on the composition of the ECM. Neutrophils transmigrated in higher number and further in geltrex mix than collagen I, and did not require an N-formyl-methionyl-leucyl-phenylalanine (fMLP) gradient for transmigration. Inhibition of neutrophil proteases inhibited neutrophil transmigration on geltrex mix, but not collagen I. These findings highlight the important role of the ECM in determining cell phenotype and response to inhibitors. Future work could adapt the ECM composition for individual diseases, producing accurate models for drug development.
Self-connected CuO–ZnO radial core–shell heterojunction nanowire arrays grown on interdigitated electrodes for visible-light photodetectors
Authors: Costas Andreea , Florica Camelia , Preda Nicoleta , Besleaga Cristina , Kuncser Andrei , Enculescu Ionut
Published: April 2022

An original photodetector system based on self-connected CuO–ZnO radial core–shell heterojunction nanowire arrays grown on metallic interdigitated electrodes, operating as visible-light photodetector was developed by combining simple preparation approaches. Metallic interdigitated electrodes were fabricated on Si/SiO2 substrates using a conventional photolithography process. Subsequently, a Cu layer was electrodeposited on top of the metallic interdigitated electrodes. The CuO nanowire arrays (core) were obtained by thermal oxidation in air of the Cu layer. Afterwards, a ZnO thin film (shell) was deposited by RF magnetron sputtering covering the surface of the CuO nanowires. The morphological, structural, compositional, optical, electrical and photoelectrical properties of the CuO nanowire arrays and CuO–ZnO core–shell nanowire arrays grown on metallic interdigitated electrodes were investigated. The performances of the devices were evaluated by assessing the figures of merit of the photodetectors based on self-connected CuO–ZnO core–shell heterojunction nanowire arrays grown on the metallic interdigitated electrodes. The radial p–n heterojunction formed between CuO and ZnO generates a type II band alignment that favors an efficient charge separation of photogenerated electron–hole pairs at the CuO–ZnO interface, suppressing their recombination and consequently enhancing the photoresponse and the photoresponsivity of the photodetectors. The electrical connections in the fabricated photodetector devices are made without any additional complex and time-consuming lithographic step through a self-connecting approach for CuO–ZnO core–shell heterojunction nanowire arrays grown directly onto the Ti/Pt metallic interdigitated electrodes. Therefore, the present study provides an accessible path for employing low dimensional complex structures in functional optoelectronic devices such as photodetectors.
Clinical significance of baseline Pan-Immune-Inflammation Value and its dynamics in metastatic colorectal cancer patients under first-line chemotherapy
Authors: Pérez-Martelo Martín , González-García Alejandro , Vidal-Ínsua Yolanda , Blanco-Freire Cristina , Brozos-Vázquez Elena María , Abdulkader-Nallib Ihab , Álvarez-Fernández Javier , Lázare-Iglesias Héctor , García-Martínez Carolina , Betancor Yoel Z. , Sánchez-Ares María , Tubío Jose M. C. , Vázquez-Rivera Francisca , Candamio-Folgar Sonia , López-López Rafael , Ruiz-Bañobre Juan
Published: April 2022

Pan-Immune-Inflammation Value (PIV) has been recently proposed as a new blood-based prognostic biomarker in metastatic colorectal cancer (mCRC). Herein we aimed to validate its prognostic significance and to evaluate its utility for disease monitoring in patients with mCRC receiving first-line chemotherapy. We conducted a single-centre retrospective study involving 130 previously untreated mCRC patients under first-line standard chemotherapy in a real-world scenario. PIV was calculated as (neutrophil count × platelet count × monocyte count)/lymphocyte count at three different time-points: baseline, week 4 after therapy initiation, and at disease progression. We analyzed the influence of baseline PIV on overall survival (OS), progression-free survival (PFS), disease control rate (DCR), and overall response rate (ORR). We also explored the utility of PIV dynamics for disease monitoring. Baseline PIV high was significantly associated with worse OS in univariate [hazard ratio (HR) = 2.10, 95% CI, 1.41–3.15; p = 0.000299] and multivariate (HR = 1.82, 95% CI, 1.15–2.90; p = 0.011) analyses. Baseline PIV was also associated with worse PFS in univariate (HR = 2.04, 95% CI, 1.40–2.97; p = 0.000187) and multivariate (HR = 1.56, 95% CI, 1.05–2.31; p = 0.026) analyses. Baseline PIV was not correlated either with DCR or ORR. Regarding PIV dynamics, there was a statistically significant increase from week 4 to disease progression (p = 0.0003), which was at the expense of cases with disease control as best response (p < 0.0001). In conclusion, this study validates the prognostic significance of baseline PIV in patients with mCRC receiving first-line standard chemotherapy in a real-world scenario. Moreover, it suggests the potential utility of PIV monitoring to anticipate the disease progression among those patients who achieve initial disease control.
Universality of political corruption networks
Authors: Martins Alvaro F. , da Cunha Bruno R. , Hanley Quentin S. , Gonçalves Sebastián , Perc Matjaž , Ribeiro Haroldo V.
Published: April 2022

Corruption crimes demand highly coordinated actions among criminal agents to succeed. But research dedicated to corruption networks is still in its infancy and indeed little is known about the properties of these networks. Here we present a comprehensive investigation of corruption networks related to political scandals in Spain and Brazil over nearly three decades. We show that corruption networks of both countries share universal structural and dynamical properties, including similar degree distributions, clustering and assortativity coefficients, modular structure, and a growth process that is marked by the coalescence of network components due to a few recidivist criminals. We propose a simple model that not only reproduces these empirical properties but reveals also that corruption networks operate near a critical recidivism rate below which the network is entirely fragmented and above which it is overly connected. Our research thus indicates that actions focused on decreasing corruption recidivism may substantially mitigate this type of organized crime.
Extending UTAUT with national identity and fairness to understand user adoption of DCEP in China
Authors: Wu Bingyan , An Xiaoqing , Wang Cong , Shin Ho Young
Published: April 2022

The introduction of digital currency electronic payment (DCEP) by the Central Bank of China is conducive to the central bank's timely grasp of macroeconomic dynamics and the internationalization of RMB. As DCEP is one of the first digital currencies issued by the central bank to be used on a large scale internationally, it is necessary to conduct research on its user adoption. Therefore, this research extends the unified theory of acceptance and use of technology (UTAUT) to explore factors affecting the adoption of DCEP. The researchers cooperated with city banks that have started to use DCEP, and distributed questionnaires to users in the lobbies of these banks. A total of 295 valid questionnaires were empirically examined with Smart-PLS. The results indicate that perceived fairness, habits, social influence and national identity have significant effects on usage, with p values less than 0.05. National identity is shown to be a significant moderator of the relationships between perceived fairness, habit, perceived risk and usage, with p values less than 0.05. National identity is shown to have no moderating effect between social influence and usage, with a p value greater than 0.05. This research provides the central bank and the government with suggestions to increase user enthusiasm and reduce user perceived risks, thereby promoting the widespread use of DCEP.
Weak spin-flip scattering in Pd89Ni11 interlayer of NbN-based ferromagnetic Josephson junctions
Authors: Pham Duong , Sugimoto Riku , Oba Kenjiro , Takeshita Yuto , Li Feng , Tanaka Masamitsu , Yamashita Taro , Fujimaki Akira
Published: April 2022

We studied niobium nitride (NbN)-based π-junctions with a diluted ferromagnetic Pd89Ni11 interlayer (NbN/PdNi/NbN junctions). In the NbN/PdNi/NbN junctions with various PdNi thicknesses, we observed a non-monotonic dependence of the critical currents on PdNi thickness, indicating the effects of the exchange interaction on the superconducting order parameter. From theoretical fitting of the experimental data, we found that the NbN/PdNi/NbN junctions showed a significantly smaller degree of spin-flip scattering in the PdNi interlayer than in the CuNi interlayer of NbN/CuNi/NbN junctions reported previously. The weak spin-flip scattering leads to a longer decay length of the Josephson critical current, so the critical currents were observed over a wide range of PdNi thicknesses (10–40 nm). We also fabricated superconducting quantum interference devices (SQUIDs) including the NbN/PdNi/NbN junction, using a PdNi thickness in which the π-state was expected. A half-flux-quantum shift, as evidence of the π-state, was observed in the magnetic field-dependent critical currents of the SQUIDs. This result represents an important step towards the practical application of NbN-based π-Josephson junctions.
Apparent absence of avian malaria and malaria-like parasites in northern blue-footed boobies breeding on Isla Isabel
Authors: Roldán-Zurabián Federico , José Ruiz-López María , de la Puente Josué Martínez , Figuerola Jordi , Drummond Hugh , Ancona Sergio
Published: April 2022

Haemosporidian parasites are common in birds but are seldom reported in seabirds. The absence of vectors or genetic resistance to infection have been proposed to explain this pattern. However, screening of blood parasites in many seabirds has been done only by visual inspection of blood smears, which can miss low-intensity infections, and molecular detection of blood parasites must be supported by detection in blood smears to confirm the presence of haemosporidians and avoid false positive cases. Here, we tested for the presence of blood parasites of the generaPlasmodium,HaemoproteusandLeucocytozoon, combining inspection of blood smears and PCR-based detection methods in a highly philopatric colony of blue-footed boobies (Sula nebouxii) in the Tropical North Pacific. Our results indicate that adults in this colony are likely free of these blood parasites, probably due to unsuitable conditions for insect vectors in booby breeding sites, although potential genetic resistance of blue-footed boobies to infection deserves examination. Apparent absence of blood parasites in Isla Isabel boobies indirectly adds to the growing evidence of variation in parasite infections among avian host species that coexist locally.
Dermoscopy practice guidelines for use in telemedicine
Authors: Deda Linda Camaj , Goldberg Rebecca H. , Jamerson Taylor A. , Lee Ivy , Tejasvi Trilokraj
Published: April 2022

Teledermoscopy, or the utilization of dermatoscopic images in telemedicine, can help diagnose dermatologic disease remotely, triage lesions of concern (i.e., determine whether in-person consultation with a dermatologist is necessary, biopsy, or reassure the patient), and monitor dermatologic lesions over time. Handheld dermatoscopes, a magnifying apparatus, have become a commonly utilized tool for providers in many healthcare settings and professions and allows users to view microstructures of the epidermis and dermis. This Dermoscopy Practice Guideline reflects current knowledge in the field of telemedicine to demonstrate the correct capture, usage, and incorporation of dermoscopic images into everyday practice.
Van der Waals integration of high-κ perovskite oxides and two-dimensional semiconductors
Authors: Yang Allen Jian , Han Kun , Huang Ke , Ye Chen , Wen Wen , Zhu Ruixue , Zhu Rui , Xu Jun , Yu Ting , Gao Peng , Xiong Qihua , Renshaw Wang X.
Published: April 2022

Two-dimensional semiconductors can be used to build next-generation electronic devices with ultrascaled channel lengths. However, semiconductors need to be integrated with high-quality dielectrics—which are challenging to deposit. Here we show that single-crystal strontium titanate—a high-κperovskite oxide—can be integrated with two-dimensional semiconductors using van der Waals forces. Strontium titanate thin films are grown on a sacrificial layer, lifted off and then transferred onto molybdenum disulfide and tungsten diselenide to make n-type and p-type transistors, respectively. The molybdenum disulfide transistors exhibit an on/off current ratio of 108at a supply voltage of 1 V and a minimum subthreshold swing of 66 mV dec−1. We also show that the devices can be used to create low-power complementary metal–oxide–semiconductor inverter circuits.