bluuebunny/crossref_metadata_2025 huggingface dataset:
Physico-Chemical and Microstructural Changes during the Drying of Persimmon Fruit cv. Rojo Brillante Harvested in Two Maturity Stages
Authors: Vilhena Nariane Q. , Gil Rebeca , Llorca Empar , Moraga Gemma , Salvador Alejandra
Published: July 2020

The physico-chemical and microstructural changes of “Rojo Brillante” persimmons in two maturity stages (S1 and S2) were evaluated during air drying. The maturity stage influences moisture loss. A Moisture level of approximately 50%, a limit at which persimmons are considered semidried, was reached after 21 and 28 days for S1 and S2, respectively. Shrinkage resulting from water removal led to secondary epidermis formation concomitantly to internal flesh gelling, which was related to moisture loss and water activity changes of each fruit part. The thicker epidermis and the lower volume of gelled area inside the S1 fruits led to harder fruit compared to the S2 fruits. The microstructural study revealed parenchyma degradation during drying in both the outermost area (secondary epidermis) and internal flesh, and this process was faster in S1 than in S2. The second peel presented hollows, generated by water outflow, which were bigger in S1 and explained the faster internal dehydration in S1. During drying, slight browning occurred, as reflected in the declining color parameters (L*, h* and C*). Water removal led to soluble solids tannin reduction to non-astringency values on day 28.
Partial Purification and Characterization of Bioactive Peptides from Cooked New Zealand Green-Lipped Mussel (Perna canaliculus) Protein Hydrolyzates
Authors: Jayaprakash Ramya , Perera Conrad O.
Published: July 2020

Proteins from fresh New Zealand green-lipped mussels were hydrolyzed for 240 min using pepsin and alcalase. The extent of the hydrolysis, antioxidant, antimicrobial, and angiotensin-converting enzyme (ACE) inhibitory activities of each protein hydrolysate were investigated. Peptides obtained from pepsin hydrolysis after 30 min, named GPH, exhibited the highest antioxidant and ACE inhibitory activity, but no antimicrobial activity. Purification of the GPH using gel-filtration chromatography revealed that the protein fraction (GPH-IV*) containing peptides with a molecular weight (MW) below 5 kDa had the strongest antioxidant and ACE inhibitory activities. Further purification was done using reverse-phase HPLC (RP-HPLC) and the only major peak obtained (GPH-IV*-P2) had the highest antioxidant and ACE inhibitory activity. From this fraction, several bioactive peptides with an MW ≈ 5 kDa were identified using LC-MS and in silico analyses. This research highlights that green-lipped mussel protein hydrolysates could be used as a good source of bioactive peptides with potential therapeutic applications.
Development and Validation of a Real-Time PCR Based Assay to Detect Adulteration with Corn in Commercial Turmeric Powder Products
Authors: Oh Su Hong , Jang Cheol Seong
Published: July 2020

Turmeric, or Curcuma longa, is commonly consumed in the South East Asian countries as a medical product and as food due to its therapeutic properties. However, with increasing demand for turmeric powder, adulterated turmeric powders mixed with other cheap starch powders, such as from corn or cassava, are being distributed by food suppliers for economic benefit. Here, we developed molecular markers using quantitative real-time PCR to identify adulteration in commercial turmeric powder products. Chloroplast genes, such as matK, atpF, and ycf2, were used to design species-specific primers for C. longa and Zea mays. Of the six primer pairs designed and tested, the correlation coefficients (R2) were higher than 0.99 and slopes were −3.136 to −3.498. The efficiency of the primers was between 93.14 and 108.4%. The specificity of the primers was confirmed with ten other species, which could be intentionally added to C. longa powders or used as ingredients in complex turmeric foods. In total, 20 blind samples and 10 commercial C. longa food products were tested with the designed primer sets to demonstrate the effectiveness of this approach to detect the addition of Z. mays products in turmeric powders. Taken together, the real-time PCR assay developed here has the potential to contribute to food safety and the protection of consumer’s rights.
Growth Trajectories in Genetic Subtypes of Prader–Willi Syndrome
Authors: Shepherd Daisy A. , Vos Niels , Reid Susan M. , Godler David E. , Guzys Angela , Moreno-Betancur Margarita , Amor David J.
Published: July 2020

Prader–Willi syndrome (PWS) is a rare disorder caused by the loss of expression of genes on the paternal copy of chromosome 15q11-13. The main molecular subtypes of PWS are the deletion of 15q11-13 and non-deletion, and differences in neurobehavioral phenotype are recognized between the subtypes. This study aimed to investigate growth trajectories in PWS and associations between PWS subtype (deletion vs. non-deletion) and height, weight and body mass index (BMI). Growth data were available for 125 individuals with PWS (63 males, 62 females), of which 72 (57.6%) had the deletion subtype. There was a median of 28 observations per individual (range 2–85), producing 3565 data points distributed from birth to 18 years of age. Linear mixed models with cubic splines, subject-specific random effects and an autoregressive correlation structure were used to model the longitudinal growth data whilst accounting for the nature of repeated measures. Height was similar for males in both PWS subtypes, with non-deletion females being shorter than deletion females for older ages. Weight and BMI were estimated to be higher in the deletion subtype compared to the non-deletion subtype, with the size of difference increasing with advancing age for weight. These results suggest that individuals with deletion PWS are more prone to obesity.
Experimental Approaches in Delineating mTOR Signaling
Authors: Qian Jiayi , Su Siyuan , Liu Pengda
Published: July 2020

The mTOR signaling controls essential biological functions including proliferation, growth, metabolism, autophagy, ageing, and others. Hyperactivation of mTOR signaling leads to a plethora of human disorders; thus, mTOR is an attractive drug target. The discovery of mTOR signaling started from isolation of rapamycin in 1975 and cloning of TOR genes in 1993. In the past 27 years, numerous research groups have contributed significantly to advancing our understanding of mTOR signaling and mTOR biology. Notably, a variety of experimental approaches have been employed in these studies to identify key mTOR pathway members that shape up the mTOR signaling we know today. Technique development drives mTOR research, while canonical biochemical and yeast genetics lay the foundation for mTOR studies. Here in this review, we summarize major experimental approaches used in the past in delineating mTOR signaling, including biochemical immunoprecipitation approaches, genetic approaches, immunofluorescence microscopic approaches, hypothesis-driven studies, protein sequence or motif search driven approaches, and bioinformatic approaches. We hope that revisiting these distinct types of experimental approaches will provide a blueprint for major techniques driving mTOR research. More importantly, we hope that thinking and reasonings behind these experimental designs will inspire future mTOR research as well as studies of other protein kinases beyond mTOR.
Interplay between Metabolism, Nutrition and Epigenetics in Shaping Brain DNA Methylation, Neural Function and Behavior
Authors: Pizzorusso Tommaso , Tognini Paola
Published: July 2020

Gene expression in the brain is dramatically regulated by a variety of stimuli. While the role of neural activity has been extensively studied, less is known about the effects of metabolism and nutrition on transcriptional control mechanisms in the brain. Extracellular signals are integrated at the chromatin level through dynamic modifications of epigenetic marks, which in turn fine-tune gene transcription. In the last twenty years, it has become clear that epigenetics plays a crucial role in modulating central nervous system functions and finally behavior. Here, we will focus on the effect of metabolic signals in shaping brain DNA methylation, both during development and adulthood. We will provide an overview of maternal nutrition effects on brain methylation and behavior in offspring. In addition, the impact of different diet challenges on cytosine methylation dynamics in the adult brain will be discussed. Finally, the possible role played by the metabolic status in modulating DNA hydroxymethylation, which is particularly abundant in neural tissue, will be considered.
A New Intra-Specific and High-Resolution Genetic Map of Eggplant Based on a RIL Population, and Location of QTLs Related to Plant Anthocyanin Pigmentation and Seed Vigour
Authors: Toppino Laura , Barchi Lorenzo , Mercati Francesco , Acciarri Nazzareno , Perrone Domenico , Martina Matteo , Gattolin Stefano , Sala Tea , Fadda Stefano , Mauceri Antonio , Ciriaci Tommaso , Carimi Francesco , Portis Ezio , Sunseri Francesco , Lanteri Sergio , Rotino Giuseppe Leonardo
Published: July 2020

Eggplant is the second most important solanaceous berry-producing crop after tomato. Despite mapping studies based on bi-parental progenies and GWAS approaches having been performed, an eggplant intraspecific high-resolution map is still lacking. We developed a RIL population from the intraspecific cross ‘305E40’, (androgenetic introgressed line carrying the locus Rfo-Sa1 conferring Fusarium resistance) x ‘67/3’ (breeding line whose genome sequence was recently released). One hundred and sixty-three RILs were genotyped by a genotype-by-sequencing (GBS) approach, which allowed us to identify 10,361 polymorphic sites. Overall, 267 Gb of sequencing data were generated and ~773 M Illumina paired end (PE) reads were mapped against the reference sequence. A new linkage map was developed, including 7249 SNPs assigned to the 12 chromosomes and spanning 2169.23 cM, with iaci@liberoan average distance of 0.4 cM between adjacent markers. This was used to elucidate the genetic bases of seven traits related to anthocyanin content in different organs recorded in three locations as well as seed vigor. Overall, from 7 to 17 QTLs (at least one major QTL) were identified for each trait. These results demonstrate that our newly developed map supplies valuable information for QTL fine mapping, candidate gene identification, and the development of molecular markers for marker assisted selection (MAS) of favorable alleles.
Profile of the Nicotinic Cholinergic Receptor Alpha 7 Subunit Gene Expression is Associated with Response to Varenicline Treatment
Authors: Santos Juliana Rocha , Tomaz Paulo Roberto Xavier , Scholz Jaqueline Ribeiro , Gaya Patrícia Viviane , Abe Tânia Ogawa , Krieger José Eduardo , Pereira Alexandre Costa , Santos Paulo Caleb Júnior de Lima
Published: July 2020

Introduction: Smoking is considered the leading cause of preventable morbidity and mortality worldwide. Studies have sought to identify predictors of response to smoking cessation treatments. The aim of this study was to analyze a possible association of target gene expression for smoking cessation with varenicline. Methods: We included 74 smokers starting treatment with varenicline. Gene expression analysis was performed through the custom RT² Profiler qPCR array assay, including 17 genes. Times for sample collection were before the start of therapy (T0) and two weeks (T2) and four weeks (T4) after the start of treatment. Results: For gene expression analysis, we selected 14 patients who had success and 13 patients resistant to varenicline treatment. Success was considered to be when a patient achieved tobacco abstinence until the fourth week of treatment and resistant was when a patient had not stopped smoking as of the fourth week of treatment. We observed a significant difference for CHRNA7 gene expression: in the resistant group, samples from T2 and T4 had lower expression compared with T0 (fold change: 0.38, P = 0.007; fold change: 0.67, P = 0.004; respectively). Conclusion: This exploratory clinical study, searching for a possible predictor of effectiveness for varenicline, reaffirmed the association of the α7 nAChR subunit for nicotine dependence and smoking therapy effectiveness with varenicline.
Genetic and Genomic Landscape of Secondary and Therapy-Related Acute Myeloid Leukemia
Authors: Higgins Alexandra , Shah Mithun Vinod
Published: July 2020

A subset of acute myeloid leukemia (AML) arises either from an antecedent myeloid malignancy (secondary AML, sAML) or as a complication of DNA-damaging therapy for other cancers (therapy-related myeloid neoplasm, t-MN). These secondary leukemias have unique biological and clinical features that distinguish them from de novo AML. Over the last decade, molecular techniques have unraveled the complex subclonal architecture of sAML and t-MN. In this review, we compare and contrast biological and clinical features of de novo AML with sAML and t-MN. We discuss the role of genetic mutations, including those involved in RNA splicing, epigenetic modification, tumor suppression, transcription regulation, and cell signaling, in the pathogenesis of secondary leukemia. We also discuss clonal hematopoiesis in otherwise healthy individuals, as well as in the context of another malignancy, and how it challenges the conventional notion of sAML/t-MN. We conclude by summarizing the current and emerging treatment strategies, including allogenic transplant, in these complex scenarios.
Preservation of Historical Underground Sites in Soft Rock: A Case Example
Authors: Jovičić Vojkan , Lapčević Radojica , Bogdanović Snežana
Published: July 2020

Rehabilitation works are often needed to preserve historical underground galleries built in soft carbonate rock. The Kalemegdan fortress galleries, located in Belgrade (Serbia) were built during the 18th century using the room-and-pillars method. Instabilities have been caused by the detrition and weathering of the rock which formed the pillars. Regardless of the fact that they lost their bearing capacity, the main requirement of the authorities was that the pillars be preserved in their current state. This controversial and difficult task was achieved by the suspension of the roof in the gallery using a combination of pre-stressed geotechnical anchors and passive rock bolts. Control of the mobilization of the anchors and the geodetic survey of the target benchmarks proved that the rehabilitation was successful in the long-term. The results of the geological investigation of the site, research solutions and remedial works are described in this paper.
Geochemical Characterization and Thermal Maturation of Cerrejón Formation: Implications for the Petroleum System in the Ranchería Sub-Basin, Colombia
Authors: Cruz-Ceballos Luis Felipe , García-González Mario , Cruz-Guevara Luis Enrique , Avendaño-Sánchez Gladys Marcela
Published: July 2020

The Upper Paleocene Cerrejón Formation is a great source of coal in Colombia. The northeastern part of the Ranchería Sub-Basin sees the most intense mining activity. As a consequence, all geological studies have been concentrated on this region. Consequently, neither the distribution of the Cerrejón Formation, nor the quality and quantity of organic matter in the rest of the sub-basin is clear. In this study, we analyzed new geochemical data from Rock–Eval pyrolysis analyses and vitrinite reflectance using core samples from the ANH-CAÑABOBA-1 and ANH-CARRETALITO-1 wells. Based on this information, it was possible to classify the geochemical characteristics of the Cerrejón Formation as a source rock, particularly in the central area of the sub-basin, which had not been extensively studied before. Additionally, based on the interpretation of seismic reflection data, the numerical burial history models were reconstructed using PetroMod software, in order to understand the evolution of the petroleum system in the sub-basin. The models were calibrated with the data of maximum pyrolysis temperature (Tmax), vitrinite reflectance (%Ro), and bottom hole temperature (BHT). We infer the potential times of the generation and expulsion of hydrocarbon from the source rock.
The Role of Family Physicians in a Pandemic: A Blueprint
Authors: Lee Jie Qi , Loke Wayren , Ng Qin Xiang
Published: July 2020

Pandemics are a significant stress test for a country’s economic, political and health systems. An effective pandemic response demands a multi-pronged and multi-layered approach, comprising surveillance, containment, border control, as well as various social and community measures. In the wake of the novel coronavirus disease 2019 (COVID-19) pandemic, which has now infected more than 7 million people worldwide, strict quarantine measures are a commonplace, and a third of the world’s population have now gone into some form of lockdown. With the exception of border control, all these response measures involve the contributions of family physicians and general practitioners (GPs) in one way or another. Primary care physicians form and lead the primary care network, which in turn forms the backbone of any healthcare system. Being the first point of contact for a significant proportion of patients, primary care physicians play an essential strategic function in the fight against disease, both during peacetime and in the event of a public health crisis. In this commentary, we examine and propose some of the key roles that they play in a pandemic, drawing examples from the current COVID-19 pandemic and past experiences. COVID-19 has showed us that the world is grossly unprepared for a pandemic, both in terms of our global management and the structure of our current primary health care systems, and this should provide the impetus for us to improve.
The Potential of Introduction of Asian Vegetables in Europe
Authors: Hong Jungha , Gruda Nazim S.
Published: July 2020

Increasing longevity, along with an aging population in Europe, has caused serious concerns about diet-related chronic diseases such as obesity, diabetes, cardiovascular diseases, and certain cancers. As recently noted during the coronavirus pandemic, regular exercise and a robust immune system complemented by adequate consumption of fruit and vegetables are recommended due to their known health benefits. Although the volume of fresh vegetable consumption in the EU is barely growing, demand for diversified, nutritious, and exotic vegetables has been increasing. Therefore, the European market for fresh Asian vegetables is expected to expand across the EU member states, and the introduction of new vegetables has enormous potential. We conducted this review to address the high number and wide range of Asian vegetable species with a commercial potential for introduction into the current European vegetable market. Many of them have not received any attention yet. Four Asian vegetables: (1) Korean ginseng sprout, (2) Korean cabbage, (3) Coastal hog fennel and (4) Japanese (Chinese or Korean) angelica tree, are further discussed. All of these vegetables possess several health benefits, are increasingly in demand, are easy to cultivate, and align with current trends of the European vegetable market, e.g., vegetables having a unique taste, higher value, are decorative and small. Introducing Asian vegetables will enhance the diversity of nutritious horticultural products in Europe, associated with all their respective consumption benefits. Future research on the Asian vegetable market within Europe is needed. In addition, experimental studies of Asian vegetables under practical conditions for their production in different European environments are required. Economic, social, and ecological aspects also ought to be considered.
Implementation of a Classroom Program of Physiotherapy among Spanish Adolescents with Back Pain: A Collaborative Study
Authors: Blanco-Morales María , Abuín-Porras Vanesa , Romero-Morales Carlos , de la Cueva-Reguera Mónica , De-La-Cruz-Torres Blanca , Rodríguez-Costa Isabel
Published: July 2020

Background: The prevalence of back pain in adolescents is steadily increasing, with negative repercussions on students’ social and academic life. This study sought to improve the ergonomics and musculoskeletal health of adolescents in secondary school by implementing physiotherapy actions within the educational context. Methods: A qualitative collaborative action research approach was used, comprising 49 students, 9 teachers, 11 family members, and 9 physiotherapists. Workshops on ergonomics, stretching, and massage were held. Visual materials were developed to support the assimilation of the information given at the workshops. Data collection included field notes, reflexive diaries, in-depth interviews, and discussion groups. The data were analyzed using the Atlas.ti 6.0 program (Scientific Software Development GmbH, Berlin, Germany). Results: The presence of a physiotherapist in the school context facilitates the acquisition of healthy postural habits. All the adolescents perceived a decrease in back pain after undergoing the program. Conclusions: physiotherapy activities offer students new tools to decrease their back pain and improve their health.
Comparison of Job Quality Indices Affecting Work–Life Balance in South Korea According to Employee Gender
Authors: Choi Seung-Hye , Choi Eun Young , Lee Haeyoung
Published: July 2020

Maintaining a healthy work–life balance is important for both males and females. Nevertheless, gender segregation still exists in labor markets in South Korea. Therefore, this study aimed to investigate differences in occupational characteristics, job quality indices, and work-life balance between male and female employees. This study was a secondary analysis of the data collected through the fifth Korean Working Conditions Survey in South Korea. Generalized ordinal logistic regression analysis was carried out to investigate the associations between job quality indices and work–life balance of employees by gender. The job quality indices were different according to gender. Male employees were mainly affected by working time quality and work intensity, while female employees were affected by both these factors and by the physical environment. Therefore, strategies differentiated by gender are necessary to improve work–life balance. In particular, more careful attention should be paid to female workers’ physical environment.
Detecting the Pedestrian Shed and Walking Route Environment of Urban Parks with Open-Source Data: A Case Study in Nanjing, China
Authors: Zhou Zhenqi , Xu Zhen
Published: July 2020

The propensity for visiting urban parks is affected by the park’s attractiveness and travel convenience, where walking provides the most basic and fair access. Walking routes from residences to parks, in terms of duration and perception, have received insufficient attention in the literature, particularly in the urban form context in China. Using the case study of Xuanwu Lake Park in Nanjing, we acquire walking routes from residences to the park through open-source data scraping in order to depict the pedestrian shed and pedestrian environment reasonably along these routes. The results show that the walking routes vary significantly with regards to distance, turns, street views, and so on. Proximity to urban parks, in terms of Euclidean distance, does not necessarily correspond to actual route distance, which may have a more direct influence on travel convenience and, hence, visiting propensity. Palpable differences in green visual ratio, image elements, and points of interest along these routes may also contribute to pedestrian environmental disparity. Analyzing data obtained from an online map provides a rapid and objective approach to detect pedestrian sheds and diagnose pedestrian environments, which can facilitate urban planners and policy makers in siting new parks and assessing the service capacity of parks.
Upper and Lower Limb Strength and Body Posture in Children with Congenital Hypothyroidism: An Observational Case-Control Study
Authors: Brusa Jessica , Maggio Maria Cristina , Giustino Valerio , Thomas Ewan , Zangla Daniele , Iovane Angelo , Palma Antonio , Corsello Giovanni , Messina Giuseppe , Bellafiore Marianna
Published: July 2020

Background: Congenital hypothyroidism (CH) is an endocrine disease with a precocious significant impairment of growth and neuromotor development. Thyroid hormones are essential for central nervous system development, maturation, and myelination. Furthermore, thyroid hormone deficiency affects the function of several systems, including the musculoskeletal system. The disease has a significant incidence in the general population (1:3000–1:2000 newborns in Italy). The aim of the present study was to evaluate any differences in upper and lower limb strength, body sway, and plantar loading distribution in children with CH compared to healthy children. Methods: In this study, the case group was composed of children with CH (CHG), while the control group included healthy children (CG). Both groups comprised 19 children (CHG: female = 12; CG: female = 9). The maximum isometric handgrip strength and explosive-elastic lower limb strength were assessed with the handgrip test and the Sargent test, respectively. The stabilometric and baropodometric analyses were used to measure the Center of Pressure displacements and the plantar loading distribution between feet, respectively. The differences between groups were analyzed by a univariate analysis of covariance using as covariates weight and height with the significant level set at < 0.05. Results: We found that CHG children were shorter and thinner than CG ones (p < 0.05). No significant difference in the upper and lower limb strength was found between groups. CHG exhibited a significant greater Sway Path Length (p < 0.01) and Ellipse Surface (p < 0.05) than CG. Moreover, CHG displayed an asymmetric plantar loading distribution with a significant lower percentage in the right than in the left foot (p < 0.05). Moreover, a significant lower plantar loading percentage in the right foot of CHG than in the right foot of CG was observed (p < 0.05). Conclusions: These findings seem to suggest that CH does not affect muscle strength in early treated children. However, these patients show poor postural control ability and asymmetric plantar loading distribution. Increasing the physical activity in these children could improve their body posture.
Public Health Academic Alliance for COVID-19 Response: The Role of a National Medical Task Force in Puerto Rico
Authors: Cruz-Correa Marcia , Díaz-Toro Elba C. , Falcón Jorge L. , García-Rivera Enid J. , Guiot Humberto M. , Maldonado-Dávila Wanda T. , Martínez Karen G. , Méndez-Latalladi William , Pérez Cynthia M. , Quiñones-Feliciano Myrna L. , Reyes Juan Carlos , Rodríguez Pablo , Santana-Bagur Jorge , Torrellas Luis C. , Vázquez Dharma , Vázquez Guillermo J. , Rodríguez-Quilichini Segundo
Published: July 2020

Alliances between the government and academic communities can be a key component of the public health response to an emergency such as the coronavirus disease 2019 (COVID-19) pandemic. The Governor of Puerto Rico designated the Puerto Rico Medical Task Force (MTF) COVID-19 to provide direct guidance and evaluation of the government response to the epidemic in Puerto Rico. Several work groups were formed within the MTF to create protocols and provide evidence-based recommendations on different public health aspects. The collaboration between the academia and the government enhanced the Puerto Rican public health response and contributed to the reduction seen in the contagion curve. Healthcare services and hospitals have not reached their maximum patient care capacity and the death toll has been controlled. Incorporating a national MTF with members of the academia into the government structure was beneficial during the COVID-19 response in Puerto Rico. A similar strategy could serve as a model for other states or territories and countries in similar scenarios.
Mind over Matter: Testing the Efficacy of an Online Randomized Controlled Trial to Reduce Distraction from Smartphone Use
Authors: Throuvala Melina A. , Griffiths Mark D. , Rennoldson Mike , Kuss Daria J.
Published: July 2020

Evidence suggests a growing call for the prevention of excessive smartphone and social media use and the ensuing distraction that arises affecting academic achievement and productivity. A ten-day online randomized controlled trial with the use of smartphone apps, engaging participants in mindfulness exercises, self-monitoring and mood tracking, was implemented amongst UK university students (n = 143). Participants were asked to complete online pre- and post-intervention assessments. Results indicated high effect sizes in reduction of smartphone distraction and improvement scores on a number of self-reported secondary psychological outcomes. The intervention was not effective in reducing habitual behaviours, nomophobia, or time spent on social media. Mediation analyses demonstrated that: (i) emotional self-awareness but not mindful attention mediated the relationship between intervention effects and smartphone distraction, and (ii) online vigilance mediated the relationship between smartphone distraction and problematic social media use. The present study provides preliminary evidence of the efficacy of an intervention for decreased smartphone distraction and highlights psychological processes involved in this emergent phenomenon in the smartphone literature. Online interventions may serve as complementary strategies to reduce distraction levels and promote insight into online engagement. More research is required to elucidate the mechanisms of digital distraction and assess its implications in problematic use.
Removal of 1,4-Naphthoquinone by Birnessite-Catalyzed Oxidation: Effect of Phenolic Mediators and the Reaction Pathway
Authors: Lee Han-Saem , Hur Jin , Lee Doo-Hee , Schlautman Mark A. , Shin Hyun-Sang
Published: July 2020

This study investigated the birnessite (δ-MnO2) catalyzed oxidative removal of 1,4-naphthoquinone (1,4-NPQ) in the presence of phenolic mediators; specifically, the kinetics of 1,4-NPQ removal under various conditions was examined, and the reaction pathway of 1,4-NPQ was verified by liquid chromatography–tandem mass spectrometry (LC–MS/MS). The removal rate of 1,4-NPQ by birnessite-catalyzed oxidation (pH = 5) was faster in the presence of phenolic mediators with electron-donating substituents (pseudo-first-order initial stage rate constant (k1) = 0.380–0.733 h−1) than with electron-withdrawing substituents (k1 = 0.071–0.244 h−1), and the effect on the substituents showed a positive correlation with the Hammett constant (Σσ) (r2 = 0.85, p < 0.001). The rate constants obtained using variable birnessite loadings (0.1–1.0 g L−1), catechol concentrations (0.1–1.0 mM), and reaction sequences indicate that phenolic mediators are the major limiting factor for the cross-coupling reaction of 1,4-NPQ in the initial reaction stages, whereas the birnessite-catalyzed surface reaction acts as the major limiting factor in the later reaction stages. This was explained by the operation of two different reaction mechanisms and reaction products identified by LC-MS/MS.
The Effect of the COVID-19 Pandemic on Physicians’ Use and Perception of Telehealth: The Case of Lebanon
Authors: Helou Samar , El Helou Elie , Abou-Khalil Victoria , Wakim Jad , El Helou Jeanine , Daher Alain , El Hachem Charline
Published: July 2020

The COVID-19 pandemic forced physicians to quickly adapt and find ways to provide their usual offline services by using online tools. We aimed to understand how physicians adapted to the sudden need for telehealth and if their perception of telehealth changed due to their experience during the COVID-19 pandemic. We conducted an exploratory sequential mixed-methods study. We interviewed five Lebanese physicians and thematically analyzed the interviews. We developed a questionnaire based on the analysis results and administered it online to physicians in Lebanon. In total, 140 responses were collected. We found that, during the COVID-19 pandemic, physicians engaged in more telehealth activities in the realms of telemedicine, public awareness, continuing medical education, research, administration, and teaching. They also expanded their repertoire of information-technology tools. Our results also show that there was a significant shift in the physicians’ perceptions, indicating greater openness and willingness to adopt telehealth services. However, a significant amount of skepticism and uncertainty regarding telemedicine remains, especially concerning its efficiency, safety, and the adequacy of existing regulations. Based on our findings, we offer recommendations for health IT policy makers, developers, and researchers, to sustain the continuity of telehealth activities beyond the COVID-19 pandemic.
The Interplay of Compassion, Subjective Happiness and Proactive Strategies on Kindergarten Teachers’ Work Engagement and Perceived Working Environment Fit
Authors: De Stasio Simona , Benevene Paula , Pepe Alessandro , Buonomo Ilaria , Ragni Benedetta , Berenguer Carmen
Published: July 2020

Background: The current cross-sectional study examines a model that was designed to advance understanding of the interplay between compassion towards teachers expressed by teaching colleagues, subjective happiness, proactive strategies and kindergarten teachers’ levels of work engagement, and perceived working environment fit. Methods: The research was conducted with a sample of 319 full-time in-service kindergarten teachers at Italian public preschools—a context in which a few previous studies have been carried out. Self-report questionnaires were administered: The Subjective Happiness Scale, the Santa Clara Brief Compassion Scale, the Utrecht Work Engagement Scale, the Proactive Strategy Scale, and the Teacher-working environment fit scale. Data were analyzed by using the structural equation modelling (SEM) approach. Results: Results show that compassion and subjective happiness have a direct positive total effect on work engagement, whereas the effects of compassion and subjective happiness on experienced working environment fit suggest that the association among constructs is mediated by the role of proactive strategies. Conclusions: Based on these findings, we strongly advocate that educational policy makers and head teachers’ pay close attention to the areas of personal and collective resources and work-related well-being, with a view to effectively address the promotion of early childhood teachers’ work engagement and working environment fit.
Environmental Risks Perception Among Citizens Living Near Industrial Plants: A Cross-Sectional Study
Authors: Dettori Marco , Pittaluga Paola , Busonera Giulia , Gugliotta Carmelo , Azara Antonio , Piana Andrea , Arghittu Antonella , Castiglia Paolo
Published: July 2020

The present work is a cross-sectional study aimed at assessing the risk perception and evaluating the community outrage linked to environmental factors among a self-selected sample of citizens living in an area characterized by the presence of industrial structures of high emotional impact. An anonymous questionnaire was administered to the population by publishing a Google form URL code in local and regional newspapers and via social media. The resulting data were entered on Excel and analyzed. Qualitative variables were summarized with absolute and relative (percentage) frequencies. The results showed that the event that causes the greatest worry was air pollution, with 92.6% of the respondents stating that they perceived the problem as “very” or “quite” worrying. Furthermore, all the health problems investigated in relation to environmental quality aroused concern among the interviewees, with 93.1% believing there was a cause-effect relationship between environmental quality and health. Overall, as other studies had previously underlined, the survey shows that the perceived risks are not always in line with the real ones, Thus, it is imperative to articulate interventions aimed at offering the population objective tools to enable them to interpret the risks themselves. In this regard, a fundamental role is played by adequate communication between the competent bodies and political decision-makers and the population.
BMP-Induced MicroRNA-101 Expression Regulates Vascular Smooth Muscle Cell Migration
Authors: Park Nanju , Kang Hara
Published: July 2020

Proliferation and migration of vascular smooth muscle cells (VSMCs) are implicated in blood vessel development, maintenance of vascular homeostasis, and pathogenesis of vascular disorders. MicroRNAs (miRNAs) mediate the regulation of VSMC functions in response to microenvironmental signals. Because a previous study reported that miR-101, a tumor-suppressive miRNA, is a critical regulator of cell proliferation in vascular disease, we hypothesized that miR-101 controls important cellular processes in VSMCs. The present study aimed to elucidate the effects of miR-101 on VSMC function and its molecular mechanisms. We revealed that miR-101 regulates VSMC proliferation and migration. We showed that miR-101 expression is induced by bone morphogenetic protein (BMP) signaling, and we identified dedicator of cytokinesis 4 (DOCK4) as a novel target of miR-101. Our results suggest that the BMP–miR-101–DOCK4 axis mediates the regulation of VSMC function. Our findings help further the understanding of vascular physiology and pathology.
Tumors Resistant to Checkpoint Inhibitors Can Become Sensitive after Treatment with Vascular Disrupting Agents
Authors: Horsman Michael R. , Wittenborn Thomas R. , Nielsen Patricia S. , Elming Pernille B.
Published: July 2020

Immune therapy improves cancer outcomes, yet many patients do not respond. This pre-clinical study investigated whether vascular disrupting agents (VDAs) could convert an immune unresponsive tumor into a responder. CDF1 mice, with 200 mm3 C3H mammary carcinomas in the right rear foot, were intraperitoneally injected with combretastatin A-4 phosphate (CA4P), its A-1 analogue OXi4503, and/or checkpoint inhibitors (anti-PD-1, PD-L1, or CTLA-4 antibodies), administered twice weekly for two weeks. Using the endpoint of tumor growth time (TGT5; time to reach five times the starting volume), we found that none of the checkpoint inhibitors (10 mg/kg) had any effect on TGT5 compared to untreated controls. However, CA4P (100 mg/kg) or OXi4503 (5–50 mg/kg) did significantly increase TGT5. This further significantly increased by combining the VDAs with checkpoint inhibitors, but was dependent on the VDA, drug dose, and inhibitor. For CA4P, a significant increase was found when CA4P (100 mg/kg) was combined with anti-PD-L1, but not with the other two checkpoint inhibitors. With OXi4503 (50 mg/kg), a significant enhancement occurred when combined with anti-PD-L1 or anti-CTLA-4, but not anti-PD-1. We observed no significant improvement with lower OXi4503 doses (5–25 mg/kg) and anti-CTLA-4, although 30% of tumors were controlled at the 25 mg/kg dose. Histological assessment of CD4/CD8 expression actually showed decreased levels up to 10 days after treatment with OXi4503 (50 mg/kg). Thus, the non-immunogenic C3H mammary carcinoma was unresponsive to checkpoint inhibitors, but became responsive in mice treated with VDAs, although the mechanism remains unclear.
Sclerostin and Vascular Pathophysiology
Authors: Catalano Antonino , Bellone Federica , Morabito Nunziata , Corica Francesco
Published: July 2020

There is cumulating evidence for a contribution of Wnt signaling pathways in multiple processes involved in atherosclerosis and vascular aging. Wnt signaling plays a role in endothelial dysfunction, in the proliferation and migration of vascular smooth muscle cells (VSMCs) and intimal thickening. Moreover, it interferes with inflammation processes, monocyte adhesion and migration, as well as with foam cell formation and vascular calcification progression. Sclerostin is a negative regulator of the canonical Wnt signaling pathway and, accordingly, the consequence of increased sclerostin availability can be disruption of the Wnt signalling cascade. Sclerostin is becoming a marker for clinical and subclinical vascular diseases and several lines of evidence illustrate its role in the pathophysiology of the vascular system. Sclerostin levels increase with aging and persist higher in some diseases (e.g., diabetes, chronic kidney disease) that are known to precipitate atherosclerosis and enhance cardiovascular risk. Current knowledge on the association between sclerostin and vascular diseases is summarized in this review.
Consumer Attitudes toward News Delivering: An Experimental Evaluation of the Use and Efficacy of Personalized Recommendations
Authors: Viana Paula , Soares Márcio , Gaio Rita , Correia Amilcar
Published: July 2020

This paper presents an experiment on newsreaders’ behavior and preferences on the interaction with online personalized news. Different recommendation approaches, based on consumption profiles and user location, and the impact of personalized news on several aspects of consumer decision-making are examined on a group of volunteers. Results show a significant preference for reading recommended news over other news presented on the screen, regardless of the chosen editorial layout. In addition, the study also provides support for the creation of profiles taking into consideration the evolution of user’s interests. The proposed solution is valid for users with different reading habits and can be successfully applied even to users with small consumption history. Our findings can be used by news providers to improve online services, thus increasing readers’ perceived satisfaction.
Analyzing Transportation Sustainability in the Canary Islands Archipelago
Authors: Castanho Rui Alexandre , Behradfar Arian , Vulevic Ana , Naranjo Gómez José Manuel
Published: July 2020

The scarcity of resources, the limited land, and the overstressing of tourism, as well as the estrangements of movement, make the insular territories relevant case studies in terms of their regional management and governance and, consequently, sustainable development. Thereby, Transportation and Infrastructures’ Sustainability in these territories is not an exception. In this regard, the present study, through exploratory tools, expects to analyze, using accessibility and connectivity indicators, the impacts over the social-economic sphere that the local Transportation and Infrastructures may deliver to the populations of the Canary Islands Archipelago. The study enables us to identify the islands of La Palma, El Hierro, Fuerteventura, and La Gomera as those with better accessibility patterns.
Environmental Display Can Buffer the Effect of Pesticides on Solitary Bees
Authors: Boff Samuel , Raizer Josué , Lupi Daniela
Published: July 2020

Environmental quality (e.g., diversity of resource availability, nesting sites, environmental display) plays an important role in an animal’s life. While homogeneous environments can restrict organisms from developing activities such as food seeking (behavioral impairment), more complex environments allow animals to perform activities with learning and behavioral perfecting outcomes. Pesticides are known to affect the learning and foraging behaviors of bees; however, little is known about the counterbalance displayed by the environment. Herein, we conducted two experiments that simulated distinct environmental displays, in which the effects of a fungicide (IndarTM 5EW-febunconazole) on solitary bee foraging activities were tested. We found that the fungicide only impaired the activities of bees in one of the studied environments. The difference in visitation rates and flower exploitation of bees between the two different environmental displays led to changes in metrics of bee–flower networks across environments. Linkage density, a metric associated with pollination efficiency that is known to be impacted by different environments, differed across environments. Our results showed that ecological interaction network metrics can differ regarding the different environmental displays. This study indicates that environmental complexity helps balance the negative effects of pesticides on solitary bees and highlights the potential use of solitary bees as model organisms for experimental simulations of environmental change.
A Review of Methane Gas Detection Sensors: Recent Developments and Future Perspectives
Authors: Aldhafeeri Tahani , Tran Manh-Kien , Vrolyk Reid , Pope Michael , Fowler Michael
Published: July 2020

Methane, the primary component of natural gas, is a significant contributor to global warming and climate change. It is a harmful greenhouse gas with an impact 28 times greater than carbon dioxide over a 100-year period. Preventing methane leakage from transmission pipelines and other oil and gas production activities is a possible solution to reduce methane emissions. In order to detect and resolve methane leaks, reliable and cost-effective sensors need to be researched and developed. This paper provides a comprehensive review of different types of methane detection sensors, including optical sensors, calorimetric sensors, pyroelectric sensors, semiconducting oxide sensors, and electrochemical sensors. The discussed material includes the definitions, mechanisms and recent developments of these sensors. A comparison between different methods, highlighting the advantages and disadvantages of each, is also presented to help address future research needs.
Test and Evaluation of Heart Rate Derived Core Temperature Algorithms for Use in NCAA Division I Football Athletes
Authors: Hagen Joshua , Himmler Aaron , Clark Joseph , Ramadan Jad , Stone Jason , Divine Jon , Mangine Robert
Published: July 2020

The purpose of this study was to assess the validity of utilizing heart rate to derive an estimate of core body temperature in American Football athletes. This was evaluated by combining commercially available Zephyr Bioharness devices, which includes an embedded estimated core temperature (ECT) algorithm, and an ingestible radio frequency core temperature pill during the highest heat injury risk timepoint of the season, summer training camp. Results showed a concordance of 0.643 and 78% of all data points fell within +/−1.0 °F. When the athletes were split into Upper (>/=6.0%) and Lower (<6.0%) body composition groups, there was a statistical improvement in accuracy with the Upper Body Fat% reaching 0.834 concordance and 93% of all values falling within +/−1.0 °F of the Gold Standard. Results suggest that heart rate derived core temperature assessments are a viable tool for heat stress monitoring in American football, but more work is required to improve on accuracy based on body composition.
Springing Analysis of a Passenger Ship in Waves
Authors: Tilander Jeremias , Patey Matthew , Hirdaris Spyros
Published: July 2020

Traditionally, the evaluation of global loads experienced by passenger ships has been based on closed-form Classification Society Rule formulae or quasi direct analysis procedures. These approaches do not account for the combined influence of hull flexibility, slenderness, and environmental actions on global dynamic response. This paper presents a procedure for the prediction of the global wave-induced loads of a medium-size passenger ship using a potential flow Flexible Fluid Structure Interaction (FFSI) model. The study compares results from direct long-term hydro-structural computations against Classification Society Rules. It is demonstrated that for the specific vessel under consideration: (a) the elastic contributions of the responses on loads are negligible as springing effects occur outside of the wave energy spectrum, (b) deviations of the order of 28% arise by way of amidships when comparing direct hydrodynamic analysis predictions encompassing IACS UR S11A hog/sag nonlinear correction factors and the longitudinal strength standard, and (c) the interpretation of the wave scatter diagram influences predictions by approximately 20%. Based on these indications, it is recommended that further parametric studies over a range of passenger ship designs could help draw unified conclusions on the total influence of global and local hydrodynamic actions on passenger ship loads and dynamic response.