bluuebunny/crossref_metadata_2025 huggingface dataset:
Bioenergetic Modeling of the Relationship Between Voltage and Electroactive Microbial Biomass Yield for Bioelectrochemical Carbon Dioxide Reduction to Methane
Authors: Ahmadi Vafa , Aryal Nabin
Published: January 2025

Optimal product synthesis in bioelectrochemical systems (BESs) requires a comprehensive understanding of the relationship between external voltage and microbial yield. While most studies assume constant growth yields or rely on empirical estimates, this study presents a novel thermodynamic model, linking anodic oxidation and cathodic carbon dioxide (CO2) reduction to methane (CH4) by growing microbial biofilm. Through integrating theoretical Gibbs free energy calculations, the model predicts electron and proton transfers for autotrophic methanogen and anode-respiring bacteria (ARB) growth, accounting for varying applied voltages and substrate concentrations. The findings identify an optimal applied cathodic potential of −0.3 V vs. the standard hydrogen electrode (SHE) for maximizing CH4 production under standard conditions (pH 7, 25 °C, 1 atm) regardless of ohmic losses. The model bridges the stoichiometry of anodic and cathodic biofilms, addressing research gaps in simulating anodic and cathodic biofilm growth simultaneously. Additionally, sensitivity analyses reveal that lower substrate concentrations require more negative voltages than standard condition to stimulate microbial growth. The model was validated using experimental data, demonstrating reasonable predictions of biomass growth and CH4 yield under different operating voltages in a multi substrate system. The results show that higher voltage inputs increase biomass yield while reducing CH4 output due to non-optimal voltage. This validated model provides a tool for optimizing BES performance to enhance CH4 recovery and biofilm stability. These insights contribute to finding optimum voltage for the highest CH4 production for energy efficient CO2 reduction for scaling up BES technology.
Functional Kimchi Beverage Enhanced with γ-Aminobutyric Acid (GABA) Through Serial Co-Fermentation Using Leuconostoc citreum S5 and Lactiplantibacillus plantarum KS2020
Authors: Kwon Min-Jeong , Kim Ji-Eun , Lee Sam-Pin
Published: January 2025

A plant-based beverage enhanced with GABA was developed through serial co-fermentation using Leuconostoc citreum S5 and Lactiplantibacillus plantarum KS2020. The first lactic acid fermentation was performed by Leu. citreum S5 with a vegetable mixture consisting of sliced radish, ginger, garlic, red pepper, bell pepper, and sucrose. The viable cell count of Leu. citreum S5 increased to 9.11–9.42 log CFU/mL with higher sucrose contents, indicating the highest value of 9.42 log CFU/mL at 5% sucrose on day 1. Mannitol and dextran production levels in the first fermented vegetable mixture were 6.66–14.54 mg/mL and 0.44–2.26%, respectively. A higher sucrose content produced more dextran, resulting in a concomitant increase in viscosity of 49.4 mPa·s. The second co-fermentation for the kimchi beverage base was performed by Lb. plantarum KS2020 for 5 days, resulting in 8.22–9.60 log CFU/mL. The pH of the co-fermented kimchi beverage base increased to 6.19–9.57 with an increasing monosodium glutamate (MSG) content (3–7%), while titratable acidity significantly decreased to 0.0–0.8%. The final co-fermented kimchi beverage base was enriched with 2.6% GABA. Consequently, a GABA kimchi beverage base with probiotics, a red pigment, and a pleasant flavor was developed using only vegetable ingredients by serial co-fermentation using lactic acid bacteria.
Composite Flours Based on Black Lentil Seeds and Sprouts with Nutritional, Phytochemical and Rheological Impact on Bakery/Pastry Products
Authors: (Neagu) Dragomir Christine , Dossa Sylvestre , Jianu Călin , Cocan Ileana , Radulov Isidora , Berbecea Adina , Radu Florina , Alexa Ersilia
Published: January 2025

This paper aimed to study the nutritional, phytochemical and rheological properties of some composite flours based on wheat flour (WF) mixed with non-germinated (LF) and sprouted lentil flour (SLF), in order to fortify the wheat flour and to obtain functional bakery/pastry products. The composite flours based on wheat flour and bean lentil flour (BLWF) and sprouted lentil flour (SLWF) were analyzed from the point of view of proximate composition (proteins, lipids, total carbohydrates, and minerals), content of individual and total polyphenols (TPC), as well as the contents of macro and microelements. For use in baking/pastries, the composite flours were tested from the point of view of rheological behavior using the MIXOLAB system, and the profiles obtained were compared with those of bread and biscuit. The results indicated that fortifying wheat flour with lentil flour, both in non-germinated and sprouted forms, increased the protein by 0.6–35.2% and mineral content of the samples and decreased the lipids by 8.3–43.2% and the carbohydrates by 2.8–9.4%. The total polyphenol content (TPC) increased by fortifying the wheat flour with non-germinated and sprouted lentil flour, the increase being between 39.2–131.4%. Regarding individual polyphenols, nine polyphenols were determined, of which epicatechin (46.979 mg/kg) and quercetin (45.95 mg/kg) were identified in the highest concentration in the composite flours. The increase in micronutrient intake by fortifying wheat flour with black lentil flour in both germinated and ungerminated form is more significant compared to the increases recorded in the case of the main macronutrients (Ca, Na, Mg, and K). The micronutrients increased in the composite flours in the order: Cu < Zn < Fe < Mn. The MIXOLAB profile highlighted that black lentil flour, although having a higher absorption index than that recommended for biscuit production, would improve the stability of the dough.
The Effect of the Glucosinolate Sinigrin on Alterations in Molecular Biomarkers of the Myocardium in Swiss Mice
Authors: Ferara Nikola , Balta Vedran , Đikić Domagoj , Odeh Dyana , Mojsović-Ćuić Ana , Feher Turković Lana , Dilber Dario , Beletić Anđelo , Landeka Jurčević Irena , Šola Ivana
Published: January 2025

Glucosinolates are chemically stable compounds that exhibit biological activity in the body following hydrolysis catalyzed by the enzyme myrosinase. While existing in vitro and in vivo studies suggest that the hydrolysis products of glucosinolates predominantly exert beneficial effects in both human and animal organisms, some studies have found that the excessive consumption of glucosinolates may lead to toxic and anti-nutritional effects. Given that glucosinolates are primarily ingested in the human diet through dietary supplements and commercially available cruciferous vegetables, we investigated the in vivo effects of the glucosinolate sinigrin on molecular markers in the myocardia of healthy Swiss mice. This study aims to elucidate whether sinigrin induces positive or negative physiological effects in mammals following consumption. The alterations in myocardial parameters were assessed by measuring metabolic, inflammatory, structural, and antioxidant markers. Our findings revealed that subchronic exposure to sinigrin in the myocardia of female mice resulted in a significant increase (p ≤ 0.05) in the levels of the myokine irisin, matrix metalloproteinases (MMP-2, MMP-9), catalase (CAT), and total glutathione (tGSH), alongside a marked decrease (p ≤ 0.05) in the levels of atrial natriuretic peptide (ANP), compared to the control group consisting of both female and male mice. These results suggest that the hydrolysis products of sinigrin may exert a potentially toxic effect on the myocardial tissue of female mice and possess the capability to modulate transcription factors in vivo in a sex-dependent manner. This observation calls for further investigation into the mechanisms regulating the actions of glucosinolate hydrolysis products, their interactions with sex hormones, and the determination of permissible intake levels associated with both beneficial and adverse outcomes.
Impact of Head Position on Facial Soft Tissue Thickness: An Ultrasound Study in the Slovak Population
Authors: Kozáková Zuzana , Sulis Simona , Falbová Darina , Vorobeľová Lenka , Matláková Mária , Beňuš Radoslav , Švábová Petra
Published: January 2025

Background/Objectives: Facial soft tissue thickness (FSTT) data are extensively utilized in forensic and medical sciences, serving as a foundational element for craniofacial reconstruction and identification methods. This study aims to analyze the differences in FSTT measurements between upright and supine positions in living subjects. Methods: The study sample consisted of 121 participants aged 20 to 86 years from Slovakia. Biological sex and age data were collected. FSTT measurements were taken at eight medial facial line landmarks and eight bilateral landmarks using a non-invasive General Electric LOGIQe R7 ultrasound device. Results: The results indicate that the head position significantly influenced more than half of the landmarks, with mean differences not exceeding 1.31 mm. Most FSTT values were higher in the horizontal position. Younger males and females exhibited significant differences in FSTT across various regions, including the eye, cheek, nose and mouth, with discrepancies in the forehead, chin, and cheek regions among younger males. In older males, only the gonion region showed a significant position-related difference, while older females demonstrated substantial FSTT changes at five landmarks, with the largest difference (1.31 mm) observed at the mandible, accounting for 15.74% of the mean FSTT. Compared to younger groups, older females exhibited higher FSTT values in the upright position. Conclusions: These findings suggest that measurements in an upright position may be more suitable for facial reconstruction, as positional changes in FSTT can occur in both positive and negative directions.
Bioerosion Structures on Dinosaur Bones Probably Made by Multituberculate Mammals and Dermestid Beetles (Guichón Formation, Late Cretaceous of Uruguay)
Authors: Perea Daniel , Verde Mariano , Mesa Valeria , Soto Matías , Montenegro Felipe
Published: January 2025

We describe the first fossil traces from the skeletal remains of dinosaurs from Uruguay, from the Upper Cretaceous Guichón Formation. We describe the first biting/gnawing fossil traces reported for Uruguay, Machichnus bohemicus Mikulás et al., 2006, probably made by small tetrapods, such as multituberculate mammals. Moreover, traces likely made by sarcosaprophagous beetles, namely Cubiculum Roberts et al., 2007, cf. Cubiculum, and cf. Osteocallis Roberts et al., 2007, are described. The presence of Cubiculum and Osteocallis supports previously proposed depositional conditions in a warm and arid to semi-arid continental environment for the referred stratigraphical unit. All traces would indicate a certain period of subaerial exposure before the definitive burial of the bones.
Topology of Locally and Non-Locally Generalized Derivatives
Authors: Prodanov Dimiter
Published: January 2025

This article investigates the continuity of derivatives of real-valued functions from a topological perspective. This is achieved by the characterization of their sets of discontinuity. The same principle is applied to Gateaux derivatives and gradients in Euclidean spaces. This article also introduces a generalization of the derivatives from the perspective of the modulus of continuity and characterizes their sets of discontinuities. There is a need for such generalizations when dealing with physical phenomena, such as fractures, shock waves, turbulence, Brownian motion, etc.
Exploration of Key Factors in the Preparation of Highly Hydrophobic Silica Aerogel from Rice Husk Ash Assisted by Machine Learning
Authors: Deng Yun , Sha Ziyan , Wang Xingxing , Duan Ke , Xue Weijie , Beadham Ian , Xiao Xiaolan , Zhang Changbo
Published: January 2025

To expand the applications of hydrophobic silica aerogels derived from rice husk ash (HSA) through simple traditional methods (without adding special materials or processes), this paper employs machine learning to establish mathematical models to identify optimal conditions for extracting water glass and investigates how preparation conditions and heat treatment temperatures affect properties such as the porosity and hydrophobicity of HSA. The results indicate that the decision tree regression model provides the most accurate predictions for the extraction rate and modulus of water glass. Notably, the water contact angle of HSA produced using nitric acid as a catalyst can reach as high as 159.5°, classifying it as a superhydrophobic material. Additionally, while moderately increasing the concentration of the hydrophobic modifier enhances HSA’s hydrophobicity, it concurrently reduces its porosity. The HSA maintained hydrophobicity until 500 °C. The pore structure of HSA collapsed gradually with the increase in heat temperature. After treatment at 700 °C, HSA lost its hydrophobicity and the porous structure was severely damaged. Compared with silica aerogel using traditional silicon sources, the damage to pore structure and the crystallization occurred at lower temperatures, but the hydrophobicity remained at higher temperatures.
Role of the Molecular Mass on the Elastic Properties of Hybrid Carrageenan Hydrogels
Authors: Gonçalves Gabriela , Faria Bruno , Moraes Izabel Cristina Freitas , Hilliou Loic
Published: January 2025

A set of carrageenans produced in the potassium form and with chemical structures varying from pure iota-carrageenans to nearly pure kappa-carrageenans is submitted to ultrasonication to reduce their molecular masses Mw while maintaining a constant chemical structure and a polydispersity index around 2. The kinetics of ultrasound-induced chain scission are found to be slower for polysaccharides richer in kappa-carrageenan disaccharide units. From the elasticity of samples directly gelled in a rheometer at 1 w/v% in 0.1 M potassium chloride, a critical molecular mass Mc is identified as the mass below which no gel can be formed. Mc is found to be smaller for kappa- and kappa-2-carrageenans of the order of 0.13–0.21 MDa. The presence of more sulphated disaccharide units significantly increases Mc up to 0.28 MDa for iota-carrageenan and 0.57 MDa for a highly sulphated hybrid carrageenan. For the set of Mw and carrageenans tested, no plateau in the Mw dependence of the gels’ elasticities is found.
Neighborhood Violence Exposure and Alcohol and Tobacco Use Initiation Among Ethnic Minority Adolescents
Authors: Santiago Anna Maria , Margetis Iris
Published: January 2025

Background/Objectives: Although the extant literature has recognized the importance of neighborhood contexts for adolescent alcohol and tobacco use, less is known about the effects of exposure to neighborhood violence on the prevalence and timing of initiation across gender and race/ethnic groups. Methods: This secondary analysis of administrative and survey data from a natural experiment in Denver examines the influence of neighborhood contexts on the health and well-being of 1100 Latino/a and African American adolescents. Cox Proportional Hazard models were used to (1) estimate the effects of exposure to neighborhood violence on the prevalence and timing of adolescent alcohol and tobacco use initiation; (2) examine gender and race/ethnic variations in alcohol and tobacco use initiation after controlling for adolescent, caregiver, household, and other neighborhood characteristics; and (3) test for threshold effects. Results: Prevalence rates among all adolescents were 12.9% for alcohol use initiation and 13.7% for tobacco use initiation but were 14.6% and 17.3%, respectively, among adolescents exposed to higher levels of neighborhood violence. The average age of initiation was 16.1 and 15.6 years for alcohol and tobacco use, respectively, but 2–8 months earlier for adolescents exposed to higher levels of neighborhood violence. Heightened exposure to neighborhood violence increased the hazards of alcohol use initiation by 32% for all adolescents and 38% for adolescent males. The hazards of tobacco use initiation were 1.3 to 1.5 times higher for male, Latino/a, and African American adolescents. Exposure to neighborhood violence suggests threshold effects of diminishing returns on adolescent tobacco use initiation. Conclusions: Findings underscore the need to examine gender and race/ethnic group differences in adolescent alcohol and tobacco initiation, the multiple pathways to such use, and interventions aimed at reducing neighborhood violence.
Gait Training with Virtual Reality-Based Real-Time Feedback for Chronic Post-Stroke Patients: A Pilot Study
Authors: Kim Sunmin , Lee Yangjin , Kim Kyunghun
Published: January 2025

Background: Virtual reality-based training has been widely used for post-stroke patients due to its positive effects on functional aspects by promoting brain plasticity. Objective: This study aimed to investigate the effectiveness of gait training with virtual reality-based real-time feedback on motor function, balance, and spatiotemporal gait parameters in post-stroke patients. Methods: Fifteen patients (n = 15) with chronic stroke were randomly assigned to either the virtual reality-based real-time feedback with treadmill gait training (experimental group n = 8) or the treadmill gait training (control group n = 7). For the experimental group that participated, a treadmill, an Oculus Rift VR device, and smart insoles were used for gait training with VR-based real-time feedback. Regarding gait training with VR-based real-time feedback, the patient wore an Oculus Rift and performed gait training on a treadmill for 30 min a day, three times a week, for 5 weeks. The control group participated in treadmill gait training for 30 min a day, three times a week, for 5 weeks. Motor function was measured using the Fugl-Meyer assessment. Balance was measured using the timed up and go test and Berg balance scale. Gait performance was measured using an Optogait. The normality test was performed using the Shapiro–Wilk test, the Wilcoxon signed-rank test was used for the within-group comparison, and the Mann–Whitney U test was used for the between-group comparison. Results: In the group analyses, both groups showed significant improvements in motor function balance and gait ability. According to the pre- and post-treatment results, greater improvement in the Fugl-Meyer assessment (experimental group: 4.75 vs. control group: 1.57) was observed in the experimental group compared with the control group (p < 0.05). In balance ability, greater improvement in the timed up and go test (experimental group: −3.10 vs. control group: −1.12) and Berg balance scale (experimental group: 3.00 vs. control group: 1.71) (p < 0.05). In the spatiotemporal gait parameters, greater improvement in affected step length (5.35 vs. 2.01), stride length (3.86 vs. 1.75), affected single support (2.61 vs. 1.22), and cadence (0.07 vs. 0.02) was observed in the experimental group compared with the control group (p < 0.05). Conclusions: This study suggested the positive effects of the virtual reality-based real-time feedback with treadmill gait training on motor function, balance, and gait performance.
Research Tendency and Frontiers of Multifocal Lenses in Myopic Control in the Past Two Decades: A Bibliometric Analysis
Authors: Jiang Lingli , Yu Manrong , Cai Jiangxiong , Wang Yingying , Hu Hao , Chen Minjie
Published: January 2025

Background: This study aimed to analyze the research progress on the use of a multifocal lens for myopic control throughout the 21st century, utilizing bibliometric analysis. Methods: Publications related to multifocal lenses from 2001 to 2024 were searched on the Web of Science core collection (WoSCC) database. VOSviewer (Version 1.6.19) and Bibliometrix package (Version 4.3.0) were used to perform the bibliometric analysis. Primary information including the publication number per year, country or region, journal, keyword, and title of the literature was extracted and analyzed. Results: A total of 234 articles from 31 countries were retrieved. The number of publications related to multifocal lenses had a rapid growth phase from 2016 to 2024. The United States, Australia, China, and Spain emerged as leading contributors to the research landscape. Ophthalmic and Physiological Optics was the most popular journal in this field, and the most frequently cited article was published in Optometry and Vision Science. Myopic progression was the main topic in this research field as well as the principal keywords of emerging research hotspots. Conclusions: Our bibliometric study provides a pioneering overview of the research trends and evolution in the application of a multifocal lens for myopic control. These findings provide a deep insight into current research frontiers and hot directions, offering a valuable reference for further research.
AntiCD30-Conjugated Antibody Plus Standard BEAM as Conditioning Regimen for Autologous Hematopoietic Stem Cell Transplantation in Systemic Anaplastic Large Cell Lymphoma
Authors: Kaloyannidis Panayotis , Al-Charfli Basmah , George Biju , Khalil Charbel , Al-Moghrabi Nour , Mustafa Samar , Ibrahim Dima , Alfar Mohammed , Ibrahim Firuz , Odeh Bassam , Daryahya Mohammed , Shabo Philip
Published: January 2025

Background/objectives: The outcome of refractory/relapsed systemic Anaplastic Large Cell Lymphoma (R/R-sALCL), especially for anaplastic lymphoma kinase-1 (ALK-1)-negative disease, remains dismal even after autologous hematopoietic stem cell transplantation (AHSCT). The intensification of both salvage and conditioning regimens, without increasing the toxicity, could improve the outcome of AHSCT in R/R-sALCL. Methods: Based on the successful experience of the incorporation of antiD20 monoclonal antibodies in the treatment of B-Cell Lymphomas, we designed a salvage and conditioning regimen incorporating the antiCD30-conjugated antibody (Brentuximab Vedotin, BV) to standard chemotherapy regimens, and we describe herein the clinical course of a patient with AKL-ve, R/R-sALCL, who received salvage regimen BV + DHAP, followed by AHSCT with preparative regimen consisted of BV plus standard BEAM. Results: The novel regimen was well tolerated, and no severe adverse effects were noticed. The engraftment was prompt and successful. The patient remained in complete metabolic remission for almost 12 months post-transplant. Conclusions: The proposed treatment approach, which combines antiCD30-conjugated antibody with standard salvage and conditioning regimens, demonstrated a completely acceptable toxicity with promising efficacy.
County-Level Food Insecurity and Hepatocellular Carcinoma Risk: A Cross-Sectional Analysis
Authors: Kehm Rebecca D. , Vilfranc Chrystelle L. , McDonald Jasmine A. , Wu Hui-Chen
Published: January 2025

Food insecurity (FI) is associated with several known hepatocellular carcinoma (HCC) risk factors, but few studies have directly examined FI in association with HCC risk. We aimed to investigate whether county-level FI is associated with HCC risk. We used data from 21 registries in the Surveillance Epidemiology and End Results database to obtain county-level counts of HCC cases from 2018 to 2021. We obtained the county-level FI rates for 2018–2021 from Feeding America’s Map the Meal Gap. We used multi-level Poisson regression models with robust standard errors to calculate incidence rate ratios (IRRs) and 95% confidence intervals (CIs). Overall, a one-standard-deviation (SD) increase in county-level FI was associated with an 8% increase in HCC risk in the fully adjusted model (IRR = 1.08, 95% CI = 1.06, 1.10). When stratified by age at diagnosis, a one-SD increase in county-level FI was associated with a 2% higher risk of HCC in the ≥65 age group (IRR = 1.02, 95% CI = 1.00, 1.05) and a 15% higher risk in the <65 age group (IRR = 1.15, 95% CI = 1.11, 1.19; interaction p-value < 0.001). If confirmed in other studies, these findings support the need for interventions and policies addressing FI in populations at increased risk for HCC.
Total Fat and Fatty Acid Content in Meals Served by Independent Takeaway Outlets Participating in the Healthier Catering Commitment Initiative in London, UK
Authors: Jaworowska Agnieszka , Force Susan
Published: January 2025

Out-of-home meals are characterized by poor nutritional quality, and their intake has been linked to adverse health outcomes. Therefore, national and local government initiatives have been implemented in the UK to promote healthier out-of-home meals. However, there is limited evidence of their effectiveness. This study evaluated the fat content and fatty acid profile of takeaway meals from ‘standard’ and from Healthier Catering Commitment (HCC)-approved takeaway outlets. Meals from 14 ‘standard’ and 13 ‘HCC-awarded’ takeaways (74 meals and 26 side dishes) were analyzed for total fat and fatty acid composition. No statistically significant differences in total fat, saturated, and trans fatty acids per 100 g and per portion between HCC and standard meals were observed, except for donner kebabs. Over 70% of all meals contained more than the recommended 30% of daily fat intake from a single meal. Some meals could provide more than 50% of the recommended total fat and SFAs intake. Despite businesses participating in the healthier out-of-home meal initiative, there has not been a significant improvement in the nutritional quality of the meals they offer. Further research to develop effective approaches to support independent takeaway businesses in offering meals with improved nutritional quality is warranted.
Incidence of Tooth Loss in Remote Indigenous Populations of the Amazon Region: A 13-Year Cohort Study Before and After Belo Monte Dam
Authors: Bastos Renata Travassos da Rosa Moreira , da Costa Eduardo Oliveira , Sicilia Lucca , Normando David
Published: January 2025

Tooth loss among indigenous people in the Amazon emphasizes the need for culturally appropriate oral health interventions. The objective of this study was to analyze the incidence of tooth loss in two remote Amazon indigenous populations. This prospective cohort evaluated a total of 47 indigenous in the permanent dentition at T0 and thirteen years later (T1) from two villages, Arara-Laranjal (n = 28, mean age 16.1 and 29.9 years) and Assurini do Xingu (n = 19, mean age 15.9 and 29.5 years), of different ethnic groups. A multilevel Poisson regression model assessed the influence of village, sex, and age on tooth loss. At T0, the indigenous people had all their permanent teeth. Forty-two lost at least one tooth (89%), and a total of 172 teeth were lost at T1 at an incidence of 97% among females and 76% in males. There was no influence of ethnicity on tooth loss (p = 1.000). A lower risk of tooth loss was associated with male subjects (β = −0.50, p < 0.05) but not with age. In females (22/46.8%) and males (11/23.4%), the highest incidence of tooth loss was the lower second molars. The risk was higher among females, and there was no influence on age, village, or ethnicity. The second and first molars were the most affected teeth. These findings suggest an increase in tooth loss caused by close contact between indigenous and urban populations.
Concept, Framework, and Data Model for Geographical Soundscapes
Authors: Lu Xiu , Li Guannan , Song Xiaoqing , Zhou Liangchen , Lv Guonian
Published: January 2025

Existing concepts and frameworks of soundscapes focus on the analysis and description of the sound source but do not explore geographical environment parameters and receiver characteristics in the geographical scene. Existing soundscape data models ignore the geographical environment and receiver information, which limits the comprehensive understanding and expression of soundscapes. They cannot study the relationship between the elements related to the sound source or explore the interaction mechanism between the sound and geographical environments. From the geographical perspective, this study extends soundscape to geographical soundscape (geo-soundscape), defines geo-soundscape by the cognition of the geographical scene, analyzes and expresses the conceptual framework of soundscapes through a content hierarchy structure, and expands the characteristics of the receiver, geographical environment parameters, further-obtained geographical scene elements, and scene element description dimensions. Based on the MPEG-7 data model, this study develops a geographical-MPEG-7 data model which consists of low-, medium-, and high-level feature classes. Taking as an example soundscape data collected on a university road in Nanjing, Jiangsu Province, in a real geographical environment, the concept, framework, and data model architecture of the geo-soundscape proposed in this study are demonstrated and described to validate the completeness and feasibility of the proposed model. The results show that our basic framework for a geo-soundscape is well adapted to the Geo-MPEG-7 data model. The model can store, organize, and describe all the soundscape information containing all elements and inter-element relationships. The soundscape in the real environment is fully expressed and described. This study provides a new research direction for soundscapes from a geographical perspective and provides guidance for urban planning and landscape design.
Marine-Derived Polysaccharide Hydrogels as Delivery Platforms for Natural Bioactive Compounds
Authors: Sepe Fabrizia , Valentino Anna , Marcolongo Loredana , Petillo Orsolina , Conte Raffaele , Margarucci Sabrina , Peluso Gianfranco , Calarco Anna
Published: January 2025

Marine polysaccharide hydrogels have emerged as an innovative platform for regulating the in vivo release of natural bioactive compounds for medical purposes. These hydrogels, which have exceptional biocompatibility, biodegradability, and high water absorption capacity, create effective matrices for encapsulating different bioactive molecules. In addition, by modifying the physical and chemical properties of marine hydrogels, including cross-linking density, swelling behavior, and response to external stimuli like pH, temperature, or ionic strength, the release profile of encapsulated bioactive compounds is strictly regulated, thus maximizing therapeutic efficacy and minimizing side effects. Finally, by using naturally sourced polysaccharides in hydrogel formulations, sustainability is promoted by reducing dependence on synthetic polymers, meeting the growing demand for eco-friendly materials. This review analyzes the interaction between marine polysaccharide hydrogels and encapsulating compounds and offers examples of how bioactive molecules can be encapsulated, released, and stabilized.
Expression of AMELX, AMBN, ENAM, TUFT1, FAM83H and MMP20 Genes in Buccal Epithelial Cells from Patients with Molar Incisor Hypomineralization (MIH)—A Pilot Study
Authors: Tynior Wojciech , Hudy Dorota , Gołąbek Karolina , Raczkowska-Siostrzonek Agnieszka , Strzelczyk Joanna Katarzyna
Published: January 2025

Molar incisor hypomineralization (MIH) is a developmental defect that affects the enamel tissue of permanent teeth. Clinicians may observe a range of opacities in the affected teeth, varying from white to creamy, yellow, and brown. Of particular interest is an etiology of MIH that has not been rigorously elucidated. Researchers believe that there are many potential etiological factors with strong genetic and/or epigenetic influence. The primary factors contributing to the risk of MIH development include specific medical conditions and circumstances. These encompass prematurity, cesarean delivery, perinatal hypoxia, and various health issues such as measles, urinary tract infections, otitis media, gastrointestinal disorders, bronchitis, kidney diseases, pneumonia, and asthma. Although genetic research in this area has received substantial attention, the investigation of epigenetic factors remains comparatively underexplored. Special attention is given to genes and their protein products involved in amelogenesis. Examples of such genes are AMELX, AMBN, ENAM, TUFT1, FAM83H, and MMP20. The median relative FAM83H gene expression in the control group was 0.038 (0.031–0.061) and 0.045 (0.032–0.087) in the study group in buccal swabs. The median relative TUFT1 gene expression in the control group was 0.328 (0.247–0.456) and 0.704 (0.334–1.183) in the study group in buccal swabs. Furthermore, children with MIH had significantly higher TUFT1 expression levels compared to the control group (p-value = 0.0043). Alterations in the expression of the TUFT1 and FAM83H genes could be contributing factors to MIH pathogenesis. Nonetheless, further investigation is essential to comprehensively elucidate the roles of all analyzed genes in the pathogenesis of MIH.
Neurobehavioral Outcomes Relate to Activation Ratio in Female Carriers of Fragile X Syndrome Full Mutation: Two Pediatric Case Studies
Authors: Di Giorgio Elisa , Benavides-Varela Silvia , Porru Annamaria , Caviola Sara , Lunghi Marco , Rigo Paola , Mioni Giovanna , Calignano Giulia , Annunziata Martina , Valenza Eloisa , Liani Valentina , Beghetti Federica , Spolaor Fabiola , Bettella Elisa , Polli Roberta , Sawacha Zimi , Murgia Alessandra
Published: January 2025

Fragile X syndrome (FXS) is a genetic neurodevelopmental disorder that causes a range of developmental problems including cognitive and behavioral impairment and learning disabilities. FXS is caused by full mutations (FM) of the FMR1 gene expansions to over 200 repeats, with hypermethylation of the cytosine–guanine–guanine (CGG) tandem repeated region in its promoter, resulting in transcriptional silencing and loss of gene function. Female carriers of FM are typically less impaired than males. The Activation Ratio (AR), the fraction of the normal allele carried on the active X chromosome, is thought to play a crucial modifying role in defining phenotype severity. Here, we compare the cognitive, neuropsychological, adaptive, and behavioral profile of two FXS girls (10 and 11 years old) with seemingly identical FMR1 genotypic profile of FM but distinctive AR levels (70% vs. 30%). A multi-method protocol, combining molecular pathophysiology and phenotypical measures, parent reports, lab-based tasks, gait analyses, and eye-tracking was employed. Results showed that lower AR corresponds to worse performances in most (cognitive, neuropsychological, adaptive, behavioral, social, mathematical skills), but not all the considered areas (i.e., time perception and gait analysis). These observations underscore the importance of AR as a phenotypic modifying parameter in females affected with FXS.
Effects of Astragaloside IV and Formononetin on Oxidative Stress and Mitochondrial Biogenesis in Hepatocytes
Authors: Tran Quoc-Anh , Tran Grant Van , Velic Sanel , Xiong Hou-Mai , Kaur Jaspreet , Moosavi Zuhurr , Nguyen Phuong , Duong Nhi , Luu Vy Tran , Singh Gurjot , Bui Tram , Rose Melanie , Ho Linh
Published: January 2025

Over-accumulation of reactive oxygen species (ROS) causes hepatocyte dysfunction and apoptosis that might lead to the progression of liver damage. Sirtuin-3 (SIRT3), the main NAD+-dependent deacetylase located in mitochondria, has a critical role in regulation of mitochondrial function and ROS production as well as in the mitochondrial antioxidant mechanism. This study explores the roles of astragaloside IV (AST-IV) and formononetin (FMR) in connection with SIRT3 for potential antioxidative effects. It was shown that the condition of combined pre- and post-treatment with AST-IV or FMR at all concentrations statistically increased and rescued cell proliferation. ROS levels were not affected by pre-or post-treatment individually with AST-IV or pre-treatment with FMR; however, post-treatment with FMR resulted in significant increases in ROS in all groups. Significant decreases in ROS levels were seen when pre- and post-treatment with AST-IV were combined at 5 and 10 μM, or FMR at 5 and 20 μM. In the condition of combined pre- and post-treatment with 10 μM AST-IV, there was a significant increase in SOD activity, and the transcriptional levels of Sod2, Cat, and GPX1 in all treatment groups, which is indicative of reactive oxygen species detoxification. Furthermore, AST-IV and FMR activated PGC-1α and AMPK as well as SIRT3 expression in AML12 hepatocytes exposed to t-BHP-induced oxidative stress, especially at high concentrations of FMR. This study presents a novel mechanism whereby AST-IV and FMR yield an antioxidant effect through induction of SIRT3 protein expression and activation of an antioxidant mechanism as well as mitochondrial biogenesis and mitochondrial content and potential. The findings suggest these agents can be used as SIRT3 modulators in treating oxidative-injury hepatocytes.
In Vitro Propagation and DNA Barcode Analysis of the Threatened Herb Solanum corymbosum from La Joya Desert, Peru
Authors: Hernández-Amasifuen Angel David , Pineda-Lázaro Alexandra Jherina , Delgado-Mera Elvin , Vega-Herrera Sergio Sebastian , Fernandes Pereira Carolina , Xavier Corrêa Ronan , Guerrero-Abad Juan Carlos , Corazon-Guivin Mike Anderson
Published: January 2025

This study focused on the in vitro propagation and DNA barcoding of the endangered herb Solanum corymbosum from the La Joya Desert, Peru. The primary objective of this study was to establish a protocol for the in vitro conservation and molecular identification of these species. This study used cytokinins, specifically BAP and TDZ, for shoot regeneration from leaf explants, achieving high rates of shoot regeneration and proliferation. The optimal concentration of TDZ (1 mg/L) resulted in 88.7% regeneration. In the rooting phase, auxins, specifically, IBA and NAA, were used, achieving high rooting rates and root numbers. The optimal concentration of IBA (2 mg/L) resulted in 100% rooting and 9.8 roots per shoot. DNA barcoding with four cpDNA markers (matK, rbcL, trnL-trnF, and psbA-trnH) allowed accurate molecular identification, confirming species identity within the genus Solanum. Key results included successful in vitro propagation with high rates of shoot formation and rooting and 100% survival during acclimatization. Molecular analysis confirmed the taxonomic identification of S. corymbosum, with high percentages of similarity (97% to 100%) with other Solanum species. Phylogenetic analysis confirmed its classification within the Radicans clade, sharing its characteristics with other Andean species of the genus Solanum. This study revealed that the propagation in vitro protocol and DNA barcoding are effective tools for the conservation of S. corymbosum, offering valuable methods for the conservation and genetic study of this and other threatened plant species.
Therapeutic Strategies for MASH: An Update on Drug Candidates Under Investigation in Late-Phase Clinical Trials
Authors: Dinerman Samuel , Shu Yan
Published: January 2025

Metabolic dysfunction-associated steatohepatitis (MASH) is rapidly becoming a leading cause of hepatocellular carcinoma and end-stage liver transplantation. Characterized by hepatic steatosis, lobular inflammation, and hepatocyte ballooning, there is a dire need to develop therapeutic strategies to mitigate MASH alongside the subsequent fibrosis and cirrhosis. For years, therapeutic development for the treatment of MASH had been considered a graveyard, with various pharmacotherapies failing to achieve clinical efficacy. However, the recent Food and Drug Administration (FDA) approval of Madrigal Pharmaceuticals’ Resmetirom in the United States provides a positive step in the collective effort to eradicate MASH. Granted, with much about Resmetirom’s long-term efficacy and safety still to be determined and with the multi-factorial nature of MASH pathogenesis, continuing to evaluate alternative therapeutic options remains in the best interest of the field. Currently, therapeutics previously approved for other ailments, alongside novel therapeutics developed specifically for the treatment of MASH, are being evaluated in late-phase clinical trials. However, considering the complex nature of the disease and varying clinical outcomes to assess treatment efficacy, achieving regulatory approval as a MASH therapeutic continues to be a rigorous endeavor. In this review, we summarize notable therapeutics of various mechanistic backgrounds having achieved, or actively undergoing, late-phase clinical trials for the treatment of MASH and offer our perspectives on anti-MASH therapeutic development.
Active Hard Sample Learning for Violation Action Recognition in Power Grid Operation
Authors: Meng Lingwen , He Di , Ban Guobang , Xi Guanghui , Li Anjun , Zhu Xinshan
Published: January 2025

Power grid operation occurs in complex, dynamic environments where the timely identification of operator violations is essential for safety. Traditional methods often rely on manual supervision and rule-based detection, leading to inefficiencies. Existing deep learning approaches, while powerful, require fully labeled data and long training times, thereby increasing costs. To address these challenges, we propose an active hard sample learning method specifically for the violation action recognition of operators in power grid operation. We design a hard instance sampling module with multi-strategy fusion based on active learning to improve training efficiency. This module identifies hard samples based on the consistency of models or samples, where we develop uncertainty evaluation and the instance discrimination strategy to assess the contributions of samples effectively. We utilize ResNet50 and ViT architectures with Faster-RCNN for detection and recognition, developed using PyTorch 2.0. The dataset comprises 2000 samples, and 30% and 60% labeled data are employed. Experimental results show significant improvements in model performance and training efficiency, demonstrating the method’s effectiveness in complex power grid environments. Our approach enhances safety monitoring and advances active learning and hard sample techniques in practical applications.
Cyclodextrins’ Internal Cavity Hydration: Insights from Theory and Experiment
Authors: Angelova Silvia , Pereva Stiliyana , Dudev Todor , Spassov Tony
Published: January 2025

In this short review, recent findings from both theory and experiment regarding the process of hydration of α-, β-, and γ-cyclodextrins are summarized and critically assessed. Key important questions are addressed, including: What factors govern the number of water molecules entrapped in the internal cavity of the host macrocycle? What is the driving force behind this process? What are the “hot spots” for water entrapment inside the host cavity? What is the underlying mechanism of water hydration and dehydration of cyclodextrins? What is the role of the confined water cluster in determining the outcome of the host–guest complexation between cyclodextrins and molecules of inorganic/organic nature? To what extent does water hydration affect the crystalline structure of the cavitand?
Relationship of Thyroid Volume and Function with Carotid and Femoral Intima-Media Thickness in Euthyroid People Aged 18–65 Taking into Account the Impact of Diabetes, Hypertension, and Excess Body Mass
Authors: Jakubiak Grzegorz K. , Pawlas Natalia , Lejawa Mateusz , Morawiecka-Pietrzak Małgorzata , Zalejska-Fiolka Jolanta , Stanek Agata , Cieślar Grzegorz
Published: January 2025

Background/Objectives: The interrelationship of thyroid volume and function with features of cardiovascular dysfunction has already been investigated but some aspects remain unclear, especially in terms of subclinical cardiovascular dysfunction in euthyroid patients. Intima-media thickness (IMT) measurement in ultrasound B-mode imaging in different vascular beds (most frequently within the common carotid artery) is one of the most important tools for the detection of subclinical atherosclerosis in both clinical practice and research. This article aimed to present the results of our research on the association between the thyroid evaluation parameters and the IMT measured in both the carotid and femoral arteries in euthyroid patients aged 18 to 65 years taking into account the influence of diabetes, hypertension, and excess body mass. Methods: We performed a retrospective cross-sectional analysis of data from patients with no thyroid disease who during planned hospitalization underwent thyroid ultrasound, determination of biochemical parameters of thyroid function, and Doppler ultrasound of carotid, vertebral, and lower extremities arteries with IMT measurement. Data from 45 people (females: 57.8%) were analyzed. Results: No significant correlations were found between biochemical parameters of thyroid function and IMT parameters. Thyroid volume was found to be significantly correlated with all parameters of the IMT within the superficial femoral artery (R = 0.407, p = 0.007 for the mean IMT calculated from the left side and the right side) and with minimal IMT within the common femoral artery taken from the left side and the right side (R = 0.342, p = 0.025). Selected IMT parameters were shown to be significantly higher in patients with arterial hypertension, diabetes, obesity, or metabolic syndrome in comparison to patients without the mentioned comorbidities. Using multiple linear regression, it was confirmed that parameters related to thyroid status do not significantly affect the IMT value. A significant effect of diabetes and, to a lesser extent, hypertension on the IMT value was confirmed, especially in the femoral arteries. Conclusions: In the population of euthyroid patients, thyroid volume correlates significantly with some parameters of femoral IMT. No significant correlations were found between thyroid biochemical parameters and IMT in both carotid and femoral arteries. A significant influence of diabetes and hypertension on the IMT value was confirmed, especially in the femoral arteries.
Prioritizing Patient Selection in Clinical Trials: A Machine Learning Algorithm for Dynamic Prediction of In-Hospital Mortality for ICU Admitted Patients Using Repeated Measurement Data
Authors: Pedarzani Emma , Fogangolo Alberto , Baldi Ileana , Berchialla Paola , Panzini Ilaria , Khan Mohd Rashid , Valpiani Giorgia , Spadaro Savino , Gregori Dario , Azzolina Danila
Published: January 2025

Background: A machine learning prognostic mortality scoring system was developed to address challenges in patient selection for clinical trials within the Intensive Care Unit (ICU) environment. The algorithm incorporates Red blood cell Distribution Width (RDW) data and other demographic characteristics to predict ICU mortality alongside existing ICU mortality scoring systems like Simplified Acute Physiology Score (SAPS). Methods: The developed algorithm, defined as a Mixed-effects logistic Random Forest for binary data (MixRFb), integrates a Random Forest (RF) classification with a mixed-effects model for binary outcomes, accounting for repeated measurement data. Performance comparisons were conducted with RF and the proposed MixRFb algorithms based solely on SAPS scoring, with additional evaluation using a descriptive receiver operating characteristic curve incorporating RDW’s predictive mortality ability. Results: MixRFb, incorporating RDW and other covariates, outperforms the SAPS-based variant, achieving an area under the curve of 0.882 compared to 0.814. Age and RDW were identified as the most significant predictors of ICU mortality, as reported by the variable importance plot analysis. Conclusions: The MixRFb algorithm demonstrates superior efficacy in predicting in-hospital mortality and identifies age and RDW as primary predictors. Implementation of this algorithm could facilitate patient selection for clinical trials, thereby improving trial outcomes and strengthening ethical standards. Future research should focus on enriching algorithm robustness, expanding its applicability across diverse clinical settings and patient demographics, and integrating additional predictive markers to improve patient selection capabilities.