bluuebunny/crossref_metadata_2025 huggingface dataset:
Long-Term and Seasonal Changes in Sources of Urban Atmospheric Particulates in the Western Pacific
Authors: Hayakawa Kazuichi , Tang Ning , Toriba Akira
Published: February 2022

To reduce atmospheric pollutants, the sources need to be identified. To this end, combustion-derived particulates (Pc) in atmospheric suspended particulate matter (TSP) in ten Western Pacific cities from 1997 to 2018 were analyzed using the NP method, which we have recently developed. The method separates Pc into particulates originating from high-temperature (Ph) and low-temperature (Pl) combustion sources. Using this method, ten cities in the Asia-Pacific region were separated into three classes. Class 1: commercial cities whose major contributor to Pc was from vehicles, and which showed lower [Pc] values with larger [Ph]/[Pc] ratios. Sapporo, Kanazawa, Tokyo, Sagamihara (Japan), Shanghai (China), and Busan (Korea) were contained in this class. Class 2: cities whose main source of Pc was from coal heating, and which showed much higher winter [Pc] with larger [Pl]/[Pc] ratios. Beijing, Shenyang (China), and Vladivostok (Russia) were contained in this class. Class 3: steel manufacturing city which showed lower [Pc] with larger [Pl]/[Pc] ratio. The low [Pc] appeared to be due to elimination of Pl from coke oven plants. Only Kitakyushu was contained in this class. This study has elucidated the contribution of sources to urban atmospheric TSP in the Western Pacific that was previously unknown. Overall [Pc] was decreasing in this region, mainly due to the decreasing contribution of vehicles to Pc, but not due to a decrease in coal combustion.
Ergonomic Design of a Workplace Using Virtual Reality and a Motion Capture Suit
Authors: Kačerová Ilona , Kubr Jan , Hořejší Petr , Kleinová Jana
Published: February 2022

Musculoskeletal disorders are some of the most frequent manual work disorders. Employers worldwide pay high costs for their treatment and prevention. We present an innovative method for designing an ergonomic workplace. This method uses new technologies and supports not only ergonomics, but also a general improvement in the designing of the manufacturing process. Although many researchers claim that there is a huge potential for using new disruptive technologies like virtual reality and motion capture in ergonomics, there is still a lack of a comprehensive methodological basis for implementing these technologies. Our approach was designed using the expert group method. We can validate the manufacturing process and the ergonomics using a motion capture (MoCap) suit and a head-mounted display (HMD). There are no legislative restrictions for the tools which are used for ergonomic analyses, so we can use our outputs for workplace scoring. Firstly, we measure the anthropometrics of the proband. Then the proband is immersed in virtual reality and they go through a manufacturing process during which ergonomics data are collected. The design of a particular workplace or multiple workplaces can be validated based on the reactions, measurements, and input in real-time. After processing the data, the workplace can be adjusted accordingly. The proposed method has a time and economic benefit for workplace design, optimisation of workplace ergonomics, and shortens the time required for designing the production line layout. It also includes optional steps for validation using conventional methods. These steps were used for method validation on a representative workplace using on-site experiments. We validated it on a group of 20 healthy operators working in automotive production (age 22 to 35). A comparison study describes the classic methods of workplace ergonomics evaluation, compares the classic evaluation using biomechanical analysis, modern evaluation using a MoCap suit, and connection with virtual reality. We have proved the validity of the method using the comparison study. The results also showed other potential issues which can be further examined: like the role of peripheral vision or haptic feedback.
Tunable Plasmonic Resonance Sensor Using a Metamaterial Film in a D-Shaped Photonic Crystal Fiber for Refractive Index Measurements
Authors: Cardoso Markos Paulo , Silva Anderson O. , Romeiro Amanda F. , Giraldi Maria Thereza R. , Costa João C. W. Albuquerque , Santos José L. , Baptista José M. , Guerreiro Ariel
Published: February 2022

Subwavelength cells of metallic nanorods arrayed in a dielectric background, termed “metamaterials”, present bulk properties that are useful to control and manipulate surface plasmon resonances. Such feature finds tremendous potential in providing a broad manifold of applications for plasmonic optical sensors. In this paper, we propose a surface-plasmon-resonance-based sensor with spectral response tunable by the volume fraction of silver present in a metamaterial layer deposited on a D-shaped photonic crystal fiber. Using computational simulations, we show that sensitivity and resolution can be hugely altered by changing the amount of constituents in the metamaterial, with no further modifications in the structure of the sensor. Moreover, the designed sensor can also be applied to label the average volume fraction of silver in the metamaterial layer and then to estimate its effective constitutive parameters.
Widespread Ability of Ligninolytic Fungi to Degrade Hazardous Organic Pollutants as the Basis for the Self-Purification Ability of Natural Ecosystems and for Mycoremediation Technologies
Authors: Pozdnyakova Natalia , Dubrovskaya Ekaterina , Schlosser Dietmar , Kuznetsova Svetlana , Sigida Elena , Grinev Vyacheslav , Golubev Sergei , Kryuchkova Elena , Varese Giovanna Cristina , Turkovskaya Olga
Published: February 2022

The ability of sixteen wood- and soil-inhabiting basidiomycete strains and four ascomycete strains to degrade the most hazardous, widespread, and persistent pollutants (polycyclic aromatic hydrocarbons, oxyethylated nonylphenol, alkylphenol, anthraquinone-type synthetic dyes, and oil) was found. The disappearance of the pollutants, their main metabolites, and some adaptive properties (activities of ligninolytic enzymes, the production of emulsifying compounds and exopolysaccharides) were evaluated. The toxicity of polycyclic aromatic hydrocarbons decreased during degradation. New data were obtained regarding (1) the dependence of the completeness of polycyclic aromatic hydrocarbon degradation on the composition of the ligninolytic enzyme complex; (2) the degradation of neonol AF9-12 by higher fungi (different accessibilities of the oxyethyl chain and the aromatic ring of the molecules to different fungal genera); and (3) the production of an emulsifying agent in response to the presence in the cultivation medium of hydrophobic pollutants as the common property of wood- and soil-inhabiting basidiomycetes and ascomycetes. Promise for use in mycoremediation was shown in the wood-inhabiting basidiomycetes Pleurotus ostreatus f. Florida, Schizophyllum commune, Trametes versicolor MUT 3403, and Trametes versicolor DSM11372; the litter-decomposing basidiomycete Stropharia rugosoannulata; and the ascomycete Cladosporium herbarum. These fungi degrade a wide range of pollutants without accumulation of toxic metabolites and produce ligninolytic enzymes and emulsifying compounds.
Application of Chromatographic and Thermal Methods to Study Fatty Acids Composition and Positional Distribution, Oxidation Kinetic Parameters and Melting Profile as Important Factors Characterizing Amaranth and Quinoa Oils
Authors: Wirkowska-Wojdyła Magdalena , Ostrowska-Ligęza Ewa , Górska Agata , Bryś Joanna
Published: February 2022

Amaranth and quinoa are classed as pseudocereals that do not belong to the grass family, meaning they are not technically a grain. Both of them are seeds with tremendous nutritional value; compared to other cereals, they contain much more fat. The aim of the study was to present the parameters characterizing thermal properties of amaranth and quinoa oils, such as: oxidation induction time, oxidation kinetic parameters, and melting profile. In isolated oils, the peroxide value, oxidative stability by the Rancimat test (in 120 °C) and the pressure differential scanning calorimetry (PDSC) method (at 100, 110, 120, 130, 140 °C), fatty acids composition, and their distribution between the triacylglycerol positions were determined. The kinetic parameters of the oxidation process (activation energy, pre-exponential factor, and reaction rate constants) were calculated using the Ozawa–Flynn–Wall method and the Arrhenius equation. To measure the melting profile, the differential scanning calorimetry (DSC) method was used. Both types of seeds are a good source of unsaturated fatty acids. Induction time of oxidation suggests that amaranth oil may have better resistance to oxidation than quinoa oil. The melting characteristics of the oils show the presence of low-melting triacylglycerol fractions, mainly containing unsaturated fatty acids, which means that a small amount of energy is required to melt the fats.
Concrete Blocks Reinforced with Arundo donax Natural Fibers with Different Aspect Ratios for Application in Bioarchitecture
Authors: Manniello Canio , Cillis Giuseppe , Statuto Dina , Di Pasquale Andrea , Picuno Pietro
Published: February 2022

In recent decades, the construction industry has advanced in its use of natural green resources, such as vegetable fibers (e.g., flax, hemp, jute, etc.) added in concrete mixtures, to create building materials that are both economically and environmentally sustainable. The pricing, low energy cost, and environmental sustainability of these natural fibers are driving this interest. The quantity of fibers and the ratio of fiber length to its transverse diameter (aspect ratio) are critical characteristics that have a decisive impact on concrete’s mechanical qualities. The influence of the aspect ratio of Arundo donax fibers on the tensile characteristics of concrete blocks was specifically investigated in this study. These fibers were collected from the outer section of the stem of this plant, which grows commonly in Mediterranean locations, but that is also found all over the world. Experiments were carried out on cylindrical concrete blocks with a constant amount of fiber (1 percent by weight) and different aspect ratios: 30, 50, and 70 (mm/mm) respectively, to assess their tensile strength, even when compared with concrete blocks without any fiber addition. Tensile tests on Arundo donax fibers were also conducted, with the aim to contribute to the analysis of their interaction with cementitious matrices, and to assess differences between the various compositions. The results showed a direct impact of the aspect ratio on the final tensile strength of concrete blocks, with higher aspect ratios producing superior tensile properties.
Particle and Particle Agglomerate Size Monitoring by Scanning Probe Microscope
Authors: Gulyaev Pavel , Krenicky Tibor , Shelkovnikov Evgeny , Korshunov Aleksandr
Published: February 2022

In the present study, the use of a scanning probe microscope is described for monitoring the sizes of nanoparticles. Monitoring is the process of acquiring and analysing the set of overlapping images. The main analysis steps are image segmentation, determination of nanoparticles allocation and their sizes, determination of the overlap of images with one another, and exclusion of repeating measurements for the formation of the correct particle-size sampling. The thorough examination of commercial scanning probe microscopes, software, and image processing libraries showed that their capabilities are limited for image segmentation and determination of sizes in complex structured images. A method based on the surface curvature computation is proposed for the image segmentation (allocation of particles) and determination of particle sizes. The curvature is estimated using the surface area approximation with respect to the circumference. It is proposed to use sample displacement sensors as an aid for image stitching.
Analysis and Design of Inertia for Grid-Tied Electric Vehicle Chargers Operating as Virtual Synchronous Machines
Authors: Liu Chenming , Fang Jingyang
Published: February 2022

Renewable energy resources (RES), e.g., solar photovoltaic (PV), wind energy, and electric vehicles (EVs) are extensively integrated into modern power electronics-based power systems due to their sustainable and environmentally friendly features. This has gradually reshaped the frequency regulation structure of power systems. Specifically, the duty of frequency regulation, previously supported by synchronous generators, will be taken over by grid-tied power converters (such as EV chargers), which can be controlled as virtual synchronous machines (VSMs) to mimic the inertial response of synchronous generators. However, being different from synchronous generators, VSMs enable fast frequency regulation. As a consequence, it is identified in this paper that the elimination of frequency oscillations is possible. Therefore, instead of simply increasing inertia for oscillations suppression, the desirable inertia coefficient can be different for various cases. In order to illustrate this point, relationships between several important performance indices, such as the rate-of-change-of-frequency, frequency nadir, overshoot, settling time, and inertia coefficient, are analyzed in detail. Furthermore, a straightforward method for designing the optimal inertia coefficient for VSMs is put forward. Finally, experiment results obtained from a 1-kW three-phase VSM prototype are demonstrated to validate the effectiveness of the theoretical analysis and proposed design method.
The Effects of Photobioreactor Type on Biomass and Lipid Production of the Green Microalga Monoraphidium pusillum in Laboratory Scale
Authors: Bácsi István , Tóthfalusi Fruzsina , Márton Kamilla , B-Béres Viktória , Gonda Sándor
Published: February 2022

Mass production of microorganisms, algae among them, for new bioactive compounds and renewable innovative products is a current issue in biotechnology. The greatest challenge of basic research on this topic is to find the best solution for both physiology and scalability. In this study, the main goal was to highlight the contradictions of physiological and technological optimization in the same, relatively small, laboratory scale. The green alga Monoraphidium pusillum (Printz) Komárková-Legnorová was cultured in a conventional Erlenmeyer flask (as air bubbled in a tank-type photobioreactor) and in a hybrid (fermenter type + helical tubular type) photobioreactor of the same volume (2.8 L). Higher cell numbers from 1.7–2.3-fold, 2–2.8-fold higher dry masses, and 1.9–2.6-fold higher total lipid contents (mg·L−1) were measured in the tank reactor than in the hybrid reactor. Cultures in the conventional tank reactor were characterized with better nutrient utilization (42.8–77.7% higher phosphate uptake) and more diverse lipid composition than in the hybrid reactor. The study highlights that well-scalable arrangements and settings could be not optimal (or unsuitable in some cases) from a physiological point of view. The results suggest certain developmental directions for complex, well-scalable devices and highlight the importance of testing the gained physiological optima on these systems.
Application of High-Order Compact Difference Schemes for Solving Partial Differential Equations with High-Order Derivatives
Authors: Caban Lena , Tyliszczak Artur
Published: February 2022

In this paper, high-order compact-difference schemes involving a large number of mesh points in the computational stencils are used to numerically solve partial differential equations containing high-order derivatives. The test cases include a linear dispersive wave equation, the non-linear Korteweg–de Vries (KdV)-like equations, and the non-linear Kuramoto–Sivashinsky equation with known analytical solutions. It is shown that very high-order compact schemes, e.g., of 20th or 24th orders, cause a very fast drop in the L2 norm error, which in some cases reaches a machine precision already on relatively coarse computational meshes.
Development and Characterization of Tailored Polyurethane Foams for Shock Absorption
Authors: Noureddine Boumdouha , Zitouni Safidine , Achraf Boudiaf , Houssém Chabane , Jannick Duchet-Rumeau , Jean-François Gerard
Published: February 2022

In this paper, different types of polyurethane foams (PUR) having various chemical compositions have been produced with a specific density to monitor the microstructure as much as possible. The foam may have a preferential orientation in the cell structure. The cellular polyurethane tends to have stubborn, typical cellular systems with strong overlap reversibility. Free expansion under atmospheric pressure enables formulas to grow until they are refined. Moreover, the physicochemical characterization of the developed foams was carried out. They later are described by apparent density, Shore hardness, Raman spectroscopy analysis, X-ray diffraction analysis, FTIR, TGA, DSC, and compression tests. The detailed structural characterization was used by scanning electron microscope (SEM) and an optical microscope (MO) to visualize the alveolar polymer’s semi-opened cells, highlighting the opened-cell morphology and chemical irregularities. Polyurethane foams with different structural variables have a spectrum characterization that influences the phase separation and topography of polyurethane foam areas because their bonding capability with hydrogen depends on chain extender nature. These studies may aid in shock absorption production; a methodology of elaboration and characterization of filled polyurethane foams is proposed.
Improved U-Net with Residual Attention Block for Mixed-Defect Wafer Maps
Authors: Cha Jaegyeong , Jeong Jongpil
Published: February 2022

Detecting defect patterns in semiconductors is very important for discovering the fundamental causes of production defects. In particular, because mixed defects have become more likely with the development of technology, finding them has become more complex than can be performed by conventional wafer defect detection. In this paper, we propose an improved U-Net model using a residual attention block that combines an attention mechanism with a residual block to segment a mixed defect. By using the proposed method, we can extract an improved feature map by suppressing irrelevant features and paying attention to the defect to be found. Experimental results show that the proposed model outperforms those in the existing studies.
Factors Influencing the Capillary Water Absorption Characteristics of Concrete and Their Relationship to Pore Structure
Authors: Wang Yue , Li Liangshun , An Mingzhe , Sun Yifan , Yu Ziruo , Huang Hanfeng
Published: February 2022

The capillary absorption capacity exerts an important effect on the durability of cement-based materials and is closely related to the pore structure. In this study, a variety of cement-based specimens were examined. The capillary water absorption and pore structure of the samples were determined using a gravimetric method and mercury intrusion porosimetry (MIP), respectively. The capillary water absorption coefficients for different water–binder ratios, diverse types and dosages of mineral admixtures, and various preloads were measured. The experimental results were analyzed and compared with data available in the current literature. The test results showed that the capillary water absorption performance of cement-based materials increased with an increasing water–binder ratio, first decreased and then increased with an increasing fly ash dosage, decreased with an increasing mineral power dosage, and decreased when the preload was less than a critical value and increased rapidly when the preload was greater than the critical value. The relationship between the capillary absorption coefficient and porosity was nearly linear. Water absorption by cement-based materials mainly correlated with pore diameters in the range of 10~1000 nm. The capillary water absorption coefficient increased continuously with the increase of pore fractal dimension.
Towards Automatic Image Enhancement with Genetic Programming and Machine Learning
Authors: Correia João , Rodriguez-Fernandez Nereida , Vieira Leonardo , Romero Juan , Machado Penousal
Published: February 2022

Image Enhancement (IE) is an image processing procedure in which the image’s original information is improved, highlighting specific features to ease post-processing analyses by a human or machine. State-of-the-art image enhancement pipelines apply solutions to fixed and static constraints to solve specific issues in isolation. In this work, an IE system for image marketing is proposed, more precisely, real estate marketing, where the objective is to enhance the commercial appeal of the images, while maintaining a level of realism and similarity with the original image. This work proposes a generic image enhancement pipeline that combines state-of-the-art image processing filters, Machine Learning methods, and Evolutionary approaches, such as Genetic Programming (GP), to create a dynamic framework for Image Enhancement. The GP-based system is trained to optimize 4 metrics: Neural Image Assessment (NIMA) technical and BRISQUE, which evaluate the technical quality of the images; and NIMA aesthetics and PhotoILike, that evaluate the commercial attractiveness. It is shown that the GP model was able to find the best image quality enhancement (0.97 NIMA Aesthetics), while maintaining a high level of similarity with the original images (Structural Similarity Index Measure (SSIM) of 0.88). The framework has better performance according to the image quality metrics than the off-the-shelf image enhancement tool and the framework’s isolated parts.
Quatre Peintres Belges au Travail: Paul Haesaerts’s Film on Edgar Tytgat, Albert Dasnoy, Jean Brusselmans and Paul Delvaux (1952)
Authors: Vandekerckhove Joséphine
Published: February 2022

Belgian art historian and filmmaker Paul Haesaerts (1901–1974) made a significant contribution to the promotion of modern Flemish art. In the late 1940s, he started experimenting with the medium of film to practice a new form of lens-based art criticism. The understudied documentary Quatre peintres belges au travail (1952) presents Belgian artists Edgar Tytgat, Albert Dasnoy, Jean Brusselmans and Paul Delvaux at work in their studio. On a large sheet of glass placed in front of the camera, they each paint one of the seasons that also represent a stage in a person’s life. A close reading of this Kodachrome color film sheds light on the context of mid-century art reproductions, mass media and post-war Flemish culture. It also examines in what way this film operates as Haesaerts’s concept of cinéma critique, while raising questions as to the way Haesaerts attempted to reconcile the spatial art of painting with the temporal medium of film.
Concept Review of a Cloud-Based Smart Battery Management System for Lithium-Ion Batteries: Feasibility, Logistics, and Functionality
Authors: Tran Manh-Kien , Panchal Satyam , Khang Tran Dinh , Panchal Kirti , Fraser Roydon , Fowler Michael
Published: February 2022

Energy storage plays an important role in the adoption of renewable energy to help solve climate change problems. Lithium-ion batteries (LIBs) are an excellent solution for energy storage due to their properties. In order to ensure the safety and efficient operation of LIB systems, battery management systems (BMSs) are required. The current design and functionality of BMSs suffer from a few critical drawbacks including low computational capability and limited data storage. Recently, there has been some effort in researching and developing smart BMSs utilizing the cloud platform. A cloud-based BMS would be able to solve the problems of computational capability and data storage in the current BMSs. It would also lead to more accurate and reliable battery algorithms and allow the development of other complex BMS functions. This study reviews the concept and design of cloud-based smart BMSs and provides some perspectives on their functionality and usability as well as their benefits for future battery applications. The potential division between the local and cloud functions of smart BMSs is also discussed. Cloud-based smart BMSs are expected to improve the reliability and overall performance of LIB systems, contributing to the mass adoption of renewable energy.
Deadbug Bridging Performance in 6- to 15-Year-Old Competitive Alpine Skiers—A Cross-Sectional Study
Authors: Strutzenberger Gerda , Ellenberger Lynn , Bruhin Björn , Frey Walter O. , Scherr Johannes , Spörri Jörg
Published: February 2022

In competitive alpine skiing, a superior antirotation and rear-chain stabilization capacity is essential to constantly remain in dynamic equilibrium while skiing and to counteract the ski-specific adverse loading patterns of the back. As such, skiers’ trunk stabilization performance during deadbug bridging (DBB) exercises has been shown to be associated with both skiing performance and overuse complaints of the lower back in skiers under 16 years of age (U16). However, to date, little is known about the corresponding stabilization abilities in younger skiers, i.e., 6- to 15-year-old skiers. As part of a biomechanical field experiment during a national off-snow fitness competition, a total of 101 youth competitive alpine skiers were tested with respect to their trunk stabilization performance during DDB exercise. The maximum contralateral displacement of the pelvic drop during leg lift (DBBdisplacement) was quantified using reflective markers and a motion capture system (Vicon, Oxford, UK). Potential age group and sex differences in DBBdisplacement were assessed using analysis of variance (ANOVA) at p < 0.05. Within each subgroup, the associations of DBBdisplacement with age, anthropometrics and maturity offset were analysed using Pearson’s correlation (p < 0.05). Female skiers under 15 years of age (U15) showed better DBB performance than male U15 skiers, while there was no sex difference at the under 10-year (U10) level. In female U10 skiers, DBBdisplacement was moderately associated with body height, while in all other subgroups, no confounding associations with anthropometrics or biological maturation were found. Biomechanically quantifying DBB performance may be considered a feasible and nonconfounded screening test approach in young skiers older than 6 years. Body height may represent a confounding bias in exclusively the U10 female skier cohort and, therefore, should be considered when interpreting the test results. In summary, this study provided sport-specific normative reference data that may be of equal interest to both researchers and sport practitioners.
Transgenic Rice Plants Expressing Artificial miRNA Targeting the Rice Stripe Virus MP Gene Are Highly Resistant to the Virus
Authors: Zhou Liya , Yuan Quan , Ai Xuhong , Chen Jianping , Lu Yuwen , Yan Fei
Published: February 2022

Rice stripe virus (RSV) causes one of the most serious viral diseases of rice. RNA interference is one of the most efficient ways to control viral disease. In this study, we constructed an amiRNA targeting the RSV MP gene (amiR MP) based on the backbone sequence of the osa-MIR528 precursor, and obtained marker-free transgenic rice plants constitutively expressing amiR MP by Agrobacterium tumefaciens-mediated transformation. A transient expression assay demonstrated that dimeric amiR MP could be effectively recognized and cleaved at the target MP gene in plants. Northern blot of miRNA indicated that amiR MP-mediated viral resistance could be stably inherited. The transgenic rice plants were highly resistant to RSV (73–90%). Our research provides novel rice germplasm for RSV control.
Regulation of p53 Function by Formation of Non-Nuclear Heterologous Protein Complexes
Authors: Zavileyskiy Lev , Bunik Victoria
Published: February 2022

A transcription factor p53 is activated upon cellular exposure to endogenous and exogenous stresses, triggering either homeostatic correction or cell death. Depending on the stress level, often measurable as DNA damage, the dual outcome is supported by p53 binding to a number of regulatory and metabolic proteins. Apart from the nucleus, p53 localizes to mitochondria, endoplasmic reticulum and cytosol. We consider non-nuclear heterologous protein complexes of p53, their structural determinants, regulatory post-translational modifications and the role in intricate p53 functions. The p53 heterologous complexes regulate the folding, trafficking and/or action of interacting partners in cellular compartments. Some of them mainly sequester p53 (HSP proteins, G6PD, LONP1) or its partners (RRM2B, PRKN) in specific locations. Formation of other complexes (with ATP2A2, ATP5PO, BAX, BCL2L1, CHCHD4, PPIF, POLG, SOD2, SSBP1, TFAM) depends on p53 upregulation according to the stress level. The p53 complexes with SIRT2, MUL1, USP7, TXN, PIN1 and PPIF control regulation of p53 function through post-translational modifications, such as lysine acetylation or ubiquitination, cysteine/cystine redox transformation and peptidyl-prolyl cis-trans isomerization. Redox sensitivity of p53 functions is supported by (i) thioredoxin-dependent reduction of p53 disulfides, (ii) inhibition of the thioredoxin-dependent deoxyribonucleotide synthesis by p53 binding to RRM2B and (iii) changed intracellular distribution of p53 through its oxidation by CHCHD4 in the mitochondrial intermembrane space. Increasing knowledge on the structure, function and (patho)physiological significance of the p53 heterologous complexes will enable a fine tuning of the settings-dependent p53 programs, using small molecule regulators of specific protein–protein interactions of p53.
Nutrient Regulation of Pancreatic Islet β-Cell Secretory Capacity and Insulin Production
Authors: Rohli Kristen E. , Boyer Cierra K. , Blom Sandra E. , Stephens Samuel B.
Published: February 2022

Pancreatic islet β-cells exhibit tremendous plasticity for secretory adaptations that coordinate insulin production and release with nutritional demands. This essential feature of the β-cell can allow for compensatory changes that increase secretory output to overcome insulin resistance early in Type 2 diabetes (T2D). Nutrient-stimulated increases in proinsulin biosynthesis may initiate this β-cell adaptive compensation; however, the molecular regulators of secretory expansion that accommodate the increased biosynthetic burden of packaging and producing additional insulin granules, such as enhanced ER and Golgi functions, remain poorly defined. As these adaptive mechanisms fail and T2D progresses, the β-cell succumbs to metabolic defects resulting in alterations to glucose metabolism and a decline in nutrient-regulated secretory functions, including impaired proinsulin processing and a deficit in mature insulin-containing secretory granules. In this review, we will discuss how the adaptative plasticity of the pancreatic islet β-cell’s secretory program allows insulin production to be carefully matched with nutrient availability and peripheral cues for insulin signaling. Furthermore, we will highlight potential defects in the secretory pathway that limit or delay insulin granule biosynthesis, which may contribute to the decline in β-cell function during the pathogenesis of T2D.
SARS-CoV-2 and Smoker’s Paradox: Mediation by Ciliary Beat Frequency and Mucociliary Clearance?
Authors: Brown Ronald B.
Published: February 2022

A COVID-19 smoker’s paradox was identified during the early days of the COVID-19 pandemic—many studies reported that smokers appeared to be protected against SARS-CoV-2 infections. Conversely, other studies added to the smoker’s paradox controversy with findings of increased risk for COVID-19 in heavy smokers. Ciliary beat frequency (CBF) within the ciliated epithelium of the nasal tract can be stimulated to a higher frequency and provide increased protection against transient exposure to airway irritants. Smokers as well as non-smokers exposed to secondhand tobacco smoke were found to have higher CBFs. However, with extended exposure to irritants, persistent upregulated CBF can damage and remodel the epithelial layer with fewer protective cilia. Additionally, mucociliary clearance (MCC), the innate defense mechanism of the respiratory system, traps particles and pathogens within the mucous layer of the epithelium and propels them out of the airways through ciliary activity. However, this mechanism becomes defective as disease progresses, increasing susceptibility to viral respiratory infections. This paper proposes that a smoker’s paradox associated with SARS-CoV-2 infection in COVID-19 patients may be mediated by upregulated ciliary beating frequency and mucociliary clearance with transient exposure to tobacco smoke, and downregulated CBF and MCC with extended exposure to tobacco smoke.
Sociodemographic and Clinical Determinants of Multimorbidity of Underlying Conditions That Increase the Risk of Severe Illness from COVID-19 in Chronic Adult Individuals
Authors: Prazeres Filipe , Castro Luísa , Teixeira Andreia
Published: February 2022

Multimorbid patients represent a special population of vulnerable individuals who suffer from two or more long-term conditions. They are a very prevalent group with an increased risk of death from COVID-19. The present study aimed to identify the sociodemographic and clinical determinants of multimorbidity of underlying conditions that increase the risk of severe COVID-19 in chronic adult individuals by analyzing data from the Portuguese National Health Survey 2019. The inclusion sample consisted of 7859 adult residents in Portugal who had at least one chronic condition. The health conditions considered for multimorbidity were CKD, COPD, heart conditions, diabetes mellitus, obesity, and smoking. In Portugal, approximately 6 out of every 10 individuals with chronic diseases suffer from one or more conditions that are on the list of those at increased risk of severe COVID-19 disease, and approximately 2 out of every 10 individuals have multimorbidity. Obesity and diabetes are the most frequent risk factors. Timely interventions (e.g., regular medical follow-up for preventive health services and health information) targeting multimorbidity in males and individuals with low educational levels, a poor health status, and low functionality may help to reduce the risk of severe COVID-19 and post-COVID-19 sequelae, and to improve health in a large proportion of the population.
The Temporal Profile of Circulating miRNAs during Gestation in Overweight and Obese Women with or without Gestational Diabetes Mellitus
Authors: Sørensen Anja Elaine , van Poppel Mireille N. M. , Desoye Gernot , Simmons David , Damm Peter , Jensen Dorte Møller , Dalgaard Louise Torp ,   The DALI Core Investigator Group
Published: February 2022

Circulating non-coding microRNAs (miRNAs) are important for placentation, but their expression profiles across gestation in pregnancies, which are complicated by gestational diabetes mellitus (GDM), have not been fully established. Investigating a single time point is insufficient, as pregnancy is dynamic, involving several processes, including placenta development, trophoblast proliferation and differentiation and oxygen sensing. Thus, the aim of this study was to compare the temporal expression of serum miRNAs in pregnant women with and without GDM. This is a nested case-control study of longitudinal data obtained from a multicentric European study (the ‘DALI’ study). All women (n = 82) were overweight/obese (BMI ≥ 29 kg/m2) and were normal glucose tolerant (NGT) at baseline (before 20 weeks of gestation). We selected women (n = 41) who were diagnosed with GDM at 24–28 weeks, according to the IADPSG/WHO2013 criteria. They were matched with 41 women who remained NGT in their pregnancy. miRNA (miR-16-5p, -29a-3p, -103-3p, -134-5p, -122-5p, -223-3p, -330-3p and miR-433-3p) were selected based on their suggested importance for placentation, and measurements were performed at baseline and at 24–28 and 35–37 weeks of gestation. Women with GDM presented with overall miRNA levels above those observed for women remaining NGT. In both groups, levels of miR-29a-3p and miR-134-5p increased consistently with progressing gestation. The change over time only differed for miR-29a-3p when comparing women with GDM with those remaining NGT (p = 0.044). Our findings indicate that among overweight/obese women who later develop GDM, miRNA levels are already elevated early in pregnancy and remain above those of women who remain NGT during their pregnancy. Maternal circulating miRNAs may provide further insight into placentation and the cross talk between the maternal and fetal compartments.
Functionalized Liposome and Albumin-Based Systems as Carriers for Poorly Water-Soluble Anticancer Drugs: An Updated Review
Authors: Teixeira Sofia , Carvalho Maria Alice , Castanheira Elisabete M. S.
Published: February 2022

Cancer is one of the leading causes of death worldwide. In the available treatments, chemotherapy is one of the most used, but has several associated problems, namely the high toxicity to normal cells and the resistance acquired by cancer cells to the therapeutic agents. The scientific community has been battling against this disease, developing new strategies and new potential chemotherapeutic agents. However, new drugs often exhibit poor solubility in water, which led researchers to develop functionalized nanosystems to carry and, specifically deliver, the drugs to cancer cells, targeting overexpressed receptors, proteins, and organelles. Thus, this review is focused on the recent developments of functionalized nanosystems used to carry poorly water-soluble drugs, with special emphasis on liposomes and albumin-based nanosystems, two major classes of organic nanocarriers with formulations already approved by the U.S. Food and Drug Administration (FDA) for cancer therapeutics.
The Mitochondrial Genome in Aging and Disease and the Future of Mitochondrial Therapeutics
Authors: Saravanan Sanjana , Lewis Caitlin J. , Dixit Bhavna , O’Connor Matthew S. , Stolzing Alexandra , Boominathan Amutha
Published: February 2022

Mitochondria are intracellular organelles that utilize nutrients to generate energy in the form of ATP by oxidative phosphorylation. Mitochondrial DNA (mtDNA) in humans is a 16,569 base pair double-stranded circular DNA that encodes for 13 vital proteins of the electron transport chain. Our understanding of the mitochondrial genome’s transcription, translation, and maintenance is still emerging, and human pathologies caused by mtDNA dysfunction are widely observed. Additionally, a correlation between declining mitochondrial DNA quality and copy number with organelle dysfunction in aging is well-documented in the literature. Despite tremendous advancements in nuclear gene-editing technologies and their value in translational avenues, our ability to edit mitochondrial DNA is still limited. In this review, we discuss the current therapeutic landscape in addressing the various pathologies that result from mtDNA mutations. We further evaluate existing gene therapy efforts, particularly allotopic expression and its potential to become an indispensable tool for restoring mitochondrial health in disease and aging.
PTML Modeling for Pancreatic Cancer Research: In Silico Design of Simultaneous Multi-Protein and Multi-Cell Inhibitors
Authors: Kleandrova Valeria V. , Speck-Planche Alejandro
Published: February 2022

Pancreatic cancer (PANC) is a dangerous type of cancer that is a major cause of mortality worldwide and exhibits a remarkably poor prognosis. To date, discovering anti-PANC agents remains a very complex and expensive process. Computational approaches can accelerate the search for anti-PANC agents. We report for the first time two models that combined perturbation theory with machine learning via a multilayer perceptron network (PTML-MLP) to perform the virtual design and prediction of molecules that can simultaneously inhibit multiple PANC cell lines and PANC-related proteins, such as caspase-1, tumor necrosis factor-alpha (TNF-alpha), and the insulin-like growth factor 1 receptor (IGF1R). Both PTML-MLP models exhibited accuracies higher than 78%. Using the interpretation from one of the PTML-MLP models as a guideline, we extracted different molecular fragments desirable for the inhibition of the PANC cell lines and the aforementioned PANC-related proteins and then assembled some of those fragments to form three new molecules. The two PTML-MLP models predicted the designed molecules as potentially versatile anti-PANC agents through inhibition of the three PANC-related proteins and multiple PANC cell lines. Conclusions: This work opens new horizons for the application of the PTML modeling methodology to anticancer research.
LL-37 and Double-Stranded RNA Synergistically Upregulate Bronchial Epithelial TLR3 Involving Enhanced Import of Double-Stranded RNA and Downstream TLR3 Signaling
Authors: Bodahl Sara , Cerps Samuel , Uller Lena , Nilsson Bengt-Olof
Published: February 2022

The human host defense peptide LL-37 influences double-stranded RNA signaling, but this process is not well understood. Here, we investigate synergistic actions of LL-37 and synthetic double-stranded RNA (poly I:C) on toll-like receptor 3 (TLR3) expression and signaling, and examine underlying mechanisms. In bronchial epithelial BEAS-2B cells, LL-37 potentiated poly I:C-induced TLR3 mRNA and protein expression demonstrated by qPCR and Western blot, respectively. Interestingly, these effects were associated with increased uptake of rhodamine-tagged poly I:C visualized by immunocytochemistry. The LL-37/poly I:C-induced upregulation of TLR3 mRNA expression was prevented by the endosomal acidification inhibitor chloroquine, indicating involvement of downstream TLR3 signaling. The glucocorticoid dexamethasone reduced LL-37/poly I:C-induced TLR3 expression on both mRNA and protein levels, and this effect was associated with increased IκBα protein expression, suggesting that dexamethasone acts via attenuation of NF-κB activity. We conclude that LL-37 potentiates poly I:C-induced upregulation of TLR3 through a mechanism that may involve enhanced import of poly I:C and that LL-37/poly I:C-induced TLR3 expression is associated with downstream TLR3 signaling and sensitive to inhibition of NF-κB activity.
The lncRNAs/miR-30e/CHI3L1 Axis Is Dysregulated in Systemic Sclerosis
Authors: Dichev Valentin , Mehterov Nikolay , Kazakova Maria , Karalilova Rositsa , Batalov Anastas , Sarafian Victoria
Published: February 2022

Systemic sclerosis (SSc) is an autoimmune disease with completely undefined etiology and treatment difficulties. The expression of both protein coding and non-coding RNAs is dysregulated during disease development. We aimed to examine a possible regulatory axis implemented in the control of chitinase-3 like protein 1 (CHI3L1) or YKL-40, an inflammation-associated glycoprotein, shown to be elevated in SSc. A panel of seven miRNAs and three lncRNAs potentially involved in the control of CHI3L1 were selected on the basis of in silico analysis. TagMan assay was used to evaluate the expression levels of miRNAs and RT-qPCR for lncRNAs in white blood cells (WBCs) and plasma from SSc patients and healthy controls. Among the eight screened miRNAs, miR-30e-5p (p = 0.04) and miR-30a-5p (p = 0.01) were significantly downregulated in WBCs and plasma of SSc patients, respectively. On the contrary, the expression of the metastasis associated lung adenocarcinoma transcript 1 (MALAT1) (p = 0.044) and the Nuclear enriched abundant transcript 1 (NEAT1) (p = 0.008) in WBCs was upregulated compared to the controls. Increased levels of MALAT1 and NEAT1 could be associated with the downregulation of miR-30e-5p and miR-30a-5p expression in WBCs and plasma. We present novel data on the involvement of a possible regulatory axis lncRNAs/miR-30e/CHI3L1 in SSc and hypothesize that MALAT1 and NEAT1 could act as miR-30e-5p and miR-30a-5p decoys. This may be a reason for the increased serum levels of CHI3L1 in SSc patients.
The Introduction of a New Diagnostic Tool in Forensic Pathology: LiDAR Sensor for 3D Autopsy Documentation
Authors: Maiese Aniello , Manetti Alice Chiara , Ciallella Costantino , Fineschi Vittorio
Published: February 2022

Autopsy is a complex and unrepeatable procedure. It is essential to have the possibility of reviewing the autoptic findings, especially when it is done for medico-legal purposes. Traditional photography is not always adequate to record forensic practice since two-dimensional images could lead to distortion and misinterpretation. Three-dimensional (3D) reconstructions of autoptic findings could be a new way to document the autopsy. Besides, nowadays, smartphones and tablets equipped with a LiDAR sensor make it extremely easy to elaborate a 3D model directly in the autopsy room. Herein, a quality and trustworthiness evaluation of 3D models obtained during ten autopsies is made comparing 3D models and conventional autopsy photographic records. Three-dimensional models were realistic and accurate and allowed precise measurements. The review of the autoptic report was facilitated by the 3D model. Conclusions: The LiDAR sensor and 3D models have been demonstrated to be a valid tool to introduce some kind of reproducibility into the autoptic practice.
Ductile Moment-Resisting Timber Connections: A Review
Authors: Rebouças Arthur S. , Mehdipour Zabih , Branco Jorge M. , Lourenço Paulo B.
Published: February 2022

In the last two decades, high-rise timber buildings have been built using the glulam truss system, even with limited openings. Moment-resisting timber frames (MRTF) with semi-rigid beam-to-column connections can be an architecture-friendly way to provide a load-carrying system to vertical and horizontal loads for timber buildings. In these structures, connections of adequate ductility are crucial to ensure robustness and energy dissipation. This paper presents a review of the main types of timber beam–column moment connections with improved ductility and proposes to carry out a ductility assessment of these connections based on the most relevant ductility factors. Joints have a significant influence on the global performance of MRTF, and the application of ductile connections have improved the mechanical parameters of the timber frame. The reinforced bolted slotted-in steel plate and glued-in rods connections have similar mechanical performance, with high rotation capacity and good ultimate moment, but exhibited different failure modes under cyclic loading. The connections were classified within ductility classes. In general, the glued-in steel rods presented better results because of the high influence of steel profiles in the connection yielding. Despite the excellent mechanical behavior, the reinforced bolted slotted-in steel plate connections presented medium ductility values.
Robotic Surgery for Biliary Tract Cancer
Authors: Kone Lyonell B. , Bystrom Philip V. , Maker Ajay V.
Published: February 2022

Biliary tract cancer consists of cholangiocarcinoma (CC) and gallbladder cancer (GBC). When resectable, surgery provides the best chance at long-term survival. Unfortunately, surgery for these tumors is associated with long operative times, high morbidities, and prolonged hospital stays. Minimally invasive surgery has been shown to impact selected outcomes, including length of stay, in other diseases, and robotic surgery may offer additional advantages compared to laparoscopic surgery in treating bile duct cancers. This is a systematic review of robotic surgery for biliary tract cancer. Predetermined selection criteria were used to appraise the literature. The PRISMA guidelines were followed. In total, 20 unique articles with a total of 259 patients with biliary tract cancer undergoing robotic surgery met the inclusion criteria. For CC and GBC, respectively, the weighted average operative time was 401 and 277 min, the estimated blood loss was 348 and 260 mL, the conversion rate to open was 7 and 3.5%, the all-cause morbidity was 52 and 9.7%, the major morbidity was 12 and 4.4%, the perioperative mortality was 1.4 and 0%, the length of stay was 15 and 4.8 days, the positive margin rate was 27 and 9%, and the number of lymph nodes retrieved was 4.2 and 8. Robotic surgery for biliary tract cancer appears non-inferior to open surgery when compared to the published contemporary data. However, the current literature on the topic is of low quality, and future prospective/randomized studies are needed.