bluuebunny/crossref_metadata_2025 huggingface dataset:
High-risk group and functional subtypes of non-suicidal self-injury in young adults with mental disorders
Authors: Yan Huiru , Zhang Yuyanan , Lu Zhe , Li Mingzhu , Ge Yuqi , Mei Dongli , Kang Zhewei , Sun Yaoyao , Li Qianqian , Yan Hao , Yang Lei , Song Peihua , Shi Chuan , Shang Shaomei , Yue Weihua
Published: February 2023

Background

Identifying high-risk groups of non-suicidal self-injury (NSSI) with multiple risk factors and different functional subtypes contribute to implementing person-centered interventions.

Methods

We investigated NSSI profiles among a sample of 258 psychiatric inpatients aged 18–25 years. All participants completed well-validated measures of internal personal and external environmental characteristics. One-hundred and ninety patients reported a lifetime history of NSSI and completed an additional NSSI assessment. A k-means cluster analysis was conducted to extract characteristics of risk factors and functional subtypes. Independent sample t-test, analysis of variance and χ2 test were used to test the difference of demographic statistical factors, risk factors and functional scores among groups with different frequency of NSSI.

Results

The clustering of risk factors analyses supported 4-clusters. The proportion of repeat NSSI patients was the highest (67.1%) in the group with unfavorable personal and unfavorable environmental characteristics. Functional subtype clustering analyses supported 5-clusters. Among patients with repeated NSSI, those with depression were mainly accompanied by the “Sensation Seeking” subtype (39.7%), bipolar disorder mainly supported the “Anti-suicide” subtype (37.9%), and eating disorders were mostly “Social Influence” subtype (33.3%). There was an interaction between functional subtypes and mental disorders.

Limitations

All participants were in treatment in a psychiatric service and the results may not be generalizable to a community sample. The data included retrospective self-report which may be inaccurate due to recall bias.

Conclusion

It is necessary to identify high-risk groups of NSSI who with unfavorable personal and environmental characteristics and clinical interventions need to consider the heterogeneity of patients’ functional subtypes of NSSI.
Religiosity, stress, and depressive symptoms among nursing and medical students during the middle stage of the COVID-19 pandemic: A cross-sectional study in Morocco
Authors: Rammouz Ismail , Lahlou Laila , Salehddine Zineb , Eloumary Omar , Laaraj Hicham , Ouhamou Mina , Mouhadi Khalid , Doufik Jalal , Aalouane Rachid , Boujraf Said
Published: February 2023

Background

Recent studies on nursing and medical students showed a higher prevalence of depression and stress than the general population. Religiosity and spirituality are common in Muslim countries and are usually used as a means of coping strategy for psychological and mental disorders.

Objective

Our objective was to evaluate the association between religious actions, depressive symptoms, and stress among students of nursing education lasting 3 years and students from the first 3 years of medical education lasting 7 years. The study was conducted at Ibn Zohr University of Agadir, Morocco.

Method

A sample of different stages of nursing and medical students was recruited. Religiosity was assessed by Muslim Belief into Action (M.BIAC) scale. The depressive symptoms and stress were, respectively, assessed by the Beck Depression Inventory (BDI-II) and Perceived Stress Scale (PSS).

Results

Four hundred and thirteen students participated in this study. Our results showed a high prevalence of depressive symptoms (62.2%) and stress (66.8%). The depression scores were higher in the following subsample categories: students in the first 2 years of studies, female medical students, and nursing students with significant differences. The recorded religiosity was greater among students without depression compared to students with depression (p < 0.001). In the multivariate regression, the BIAC score demonstrated religiosity as neither a risk factor nor a protective factor of depression.

Conclusion

Religiosity constitutes a protective factor of depression and stress among nursing and medical students. This should improve the student's ability to cope with stressful situations during their training. Prospective studies are needed to further investigate this association and how religiosity improves mental health. This would contribute to improved academic performance and wellbeing among medical and nursing students.
Dynamics of social media behavior before and after SARS-CoV-2 infection
Authors: Durazzi Francesco , Pichard François , Remondini Daniel , Salathé Marcel
Published: February 2023

Introduction

Online social media have been both a field of research and a source of data for research since the beginning of the COVID-19 pandemic. In this study, we aimed to determine how and whether the content of tweets by Twitter users reporting SARS-CoV-2 infections changed over time.

Methods

We built a regular expression to detect users reporting being infected, and we applied several Natural Language Processing methods to assess the emotions, topics, and self-reports of symptoms present in the timelines of the users.

Results

Twelve thousand one hundred and twenty-one twitter users matched the regular expression and were considered in the study. We found that the proportions of health-related, symptom-containing, and emotionally non-neutral tweets increased after users had reported their SARS-CoV-2 infection on Twitter. Our results also show that the number of weeks accounting for the increased proportion of symptoms was consistent with the duration of the symptoms in clinically confirmed COVID-19 cases. Furthermore, we observed a high temporal correlation between self-reports of SARS-CoV-2 infection and officially reported cases of the disease in the largest English-speaking countries.

Discussion

This study confirms that automated methods can be used to find digital users publicly sharing information about their health status on social media, and that the associated data analysis may supplement clinical assessments made in the early phases of the spread of emerging diseases. Such automated methods may prove particularly useful for newly emerging health conditions that are not rapidly captured in the traditional health systems, such as the long term sequalae of SARS-CoV-2 infections.
Unsung heroes in health education and promotion: How Community Health Workers contribute to hypertension management
Authors: Bush Kim , Patrick Carlea , Elliott Kimberly , Morris Michael , Tiruneh Yordanos , McGaha Paul
Published: February 2023

Rural communities are noted as having poor health outcomes. Rural areas experience barriers to care primarily due to a lack of resources, including education, health insurance, transportation, and social support. Additionally, poor health outcomes are a consequence of poor health literacy skills. Community Health Workers (CHWs) are utilized as a resource to combat these issues. This study focused on a CHW led Self-Management Blood Pressure (SMBP) program offered through the University of Texas at Tyler Health Science Center. The goal of the program was to improve management of hypertension through awareness, education, navigation, advocacy, and resource assistance. The SMBP program included structured workshops and regular follow-up with participants including connections to community resources and social support. CHWs worked closely with physicians providing bi-directional feedback on referrals and engagement of communities through outreach events. Furthermore, CHWs aided to bridge cultural or linguistic gaps between service providers and community members. Data is provided indicating this CHW-led intervention played a significant role in improving hypertension through education of how to make lifestyle changes that impact overall health and quality of life. Participants gained knowledge encouraging them to create lifelong healthy habits, coping skills, stress management, self-care, and accountability. Through this innovative approach, participants thrived in the supportive and encouraging environment led by CHWs as well as improved their blood pressure management.
Australians' experiences of COVID-19 during the early months of the crisis: A qualitative interview study
Authors: Lupton Deborah , Lewis Sophie
Published: February 2023

Introduction

The COVID-19 crisis has wrought major changes to people's lives across the globe since the beginning of the outbreak in early 2020. The "Australians' Experiences of COVID-19” qualitative descriptive study was established to explore how Australians from different geographical areas and social groups experienced the COVID-19 crisis.

Methods

Three sets of semi-structured interviews, each with a diverse group of 40 adults across Australia, were completed between 2020 and 2022. This article reports findings from the first set of interviews, conducted by telephone in mid-2020.

Results

The participants discussed their experiences of living through this period, which was characterized by strong public health measures to contain the spread of COVID, including a national lockdown and border closures. Interview fieldnotes and verbatim transcripts were used to conduct an interpretive thematic analysis. The analysis is structured around the following five themes covering the quotidian and affective aspects of participants' lives in the early months of the COVID crisis: “disruption to routines;” “habituating to preventive measures;” “social isolation and loneliness;” “changes to work and education;” and “little change to life.” A sixth theme concerns how participants responded to our question about what they imagined their lives would be like after the pandemic: “imagining post-COVID life.”

Discussion

The crisis affected participants' experience of daily life variously according to such factors as their social circumstances and obligations as well as their histories of illness, making visible some of the unequal social and economic effects of the pandemic across different genders, ages, localities and socioeconomic groups. Our participants fell into three roughly equal groups: (i) those who found the lockdown and associated restrictions very difficult; (ii) those who reported feeling barely affected by these conditions; and (iii) those who found benefits to the “slowing down” of life during this period.
Breathlessness and exercise with virtual reality system in long-post-coronavirus disease 2019 patients
Authors: Stavrou Vasileios T. , Vavougios George D. , Kalogiannis Periklis , Tachoulas Konstantinos , Touloudi Evlalia , Astara Kyriaki , Mysiris Dimitrios S. , Tsirimona Glykeria , Papayianni Eirini , Boutlas Stylianos , Hassandra Mary , Daniil Zoe , Theodorakis Yannis , Gourgoulianis Konstantinos I.
Published: February 2023

Long-post-coronavirus disease-2019 (COVID-19) patients tend to claim residual symptomatology from various systems, most importantly the respiratory and central nervous systems. Breathlessness and brain fog are the main complaints. The pulmonary function pattern is consistent with restrictive defects, which, in most cases, are self-resolved, while the cognitive profile may be impaired. Rehabilitation is an ongoing field for holistic management of long-post-COVID-19 patients. Virtual reality (VR) applications may represent an innovative implementation of rehabilitation. We aimed to investigate the effect of exercise with and without the VR system and to assess further breathlessness and functional fitness indicators in long-post-COVID-19 patients with mild cognitive impairment after self-selected exercise duration using the VR system. Twenty long-post-COVID-19 patients were enrolled in our study (age: 53.9 ± 9.1 years, male: 80%, body mass index: 28.1 ± 3.1 kg/m2). Participants' anthropometric data were recorded, and they underwent pulmonary functional test evaluation as well as sleep quality and cognitive assessment. The participants randomly exercised with and without a VR system (VR vs. no-VR) and, later, self-selected the exercise duration using the VR system. The results showed that exercise with VR resulted in a lower dyspnea score than exercise without VR. In conclusion, VR applications seem to be an attractive and safe tool for implementing rehabilitation. They can enhance performance during exercise and benefit patients with both respiratory and cognitive symptoms.
Depression and suicidal ideation among individuals with type-2 diabetes mellitus, a cross-sectional study from an urban slum area of Karachi, Pakistan
Authors: Sharif Hina , Jan Shah Sumaya , Sharif Sana , Seemi Tooba , Naeem Hira , Jawed Zahida
Published: February 2023

Background

Suicidal thoughts and depression are associated with patients with diabetes, especially patients with low socioeconomic backgrounds and prolonged illness.

Objective

We aimed to estimate suicidal thoughts and depression among patients with type 2 diabetes (T2D) in the slums of Karachi.

Methods

This cross-sectional study was conducted across 38 locations in the slums of Karachi to understand depression, suicidal thoughts, and other supporting factors of depression associated with T2D. The three-item Oslo Social Support Scale, the Patient Health Questionnaire-9 (PHQ-9) scale, and the Ask Suicide Screening Questions were used to screen the patients.

Results

A total of 504 study participants were interviewed, with a response rate of 98%. The prevalence of depression among patients with diabetes was 30.83%, and suicidal ideation was 20.39%. In the final multivariate analysis, being socioeconomically poor, physically disabled, and having poor social support were independent predictors of depression.

Conclusion

Diabetes, low socioeconomic level, a lack of social support, and physical disability were all linked to depression. Therefore, trained health providers should conduct an early depression-focused routine screening for patients with diabetes.
NP-hard but no longer hard to solve? Using quantum computing to tackle optimization problems
Authors: Au-Yeung Rhonda , Chancellor Nicholas , Halffmann Pascal
Published: February 2023

In the last decade, public and industrial research funding has moved quantum computing from the early promises of Shor’s algorithm through experiments to the era of noisy intermediate scale quantum devices (NISQ) for solving real-world problems. It is likely that quantum methods can efficiently solve certain (NP-) hard optimization problems where classical approaches fail. In our perspective, we examine the field of quantum optimization, that is, solving optimization problems using quantum computers. We provide an entry point to quantum optimization for researchers from each topic, optimization or quantum computing, by demonstrating advances and obstacles with a suitable use case. We give an overview on problem formulation, available algorithms, and benchmarking. Although we show a proof-of-concept rather than a full benchmark between classical and quantum methods, this gives an idea of the current quality and capabilities of quantum computers for optimization problems. All observations are incorporated in a discussion on some recent quantum optimization breakthroughs, current status, and future directions.
Patricia Lacks' contributions to evidence-based practice for insomnia
Authors: Kay Daniel B. , Wolfson Amy R.
Published: February 2023

In the 1980s, Patricia Lacks (February 22, 1941–December 3, 2016) began a systematic program of research at Washington University in St. Louis to identify the causes of insomnia and effective treatments. Her treatment outcomes research culminated in the publication of the first detailed cognitive-behavioral treatment manual for insomnia, Behavioral Treatment for Persistent Insomnia. This paper focuses on the history of Dr. Lacks and her contributions to the field of insomnia research and practice.
Nitrogen removal performance and bacterial community analysis of a multistage step-feeding tidal flow constructed wetland
Authors: Khajah Mishari , Bydalek Franciszek , Babatunde Akintunde O. , Al-Matouq Abdullah , Wenk Jannis , Webster Gordon
Published: February 2023

A multistage mesocosm vertical flow constructed wetland system was designed to treat synthetic domestic wastewater with a high nitrogen (N) load. The study aim was to determine the impact of design and operational variables on N removal efficiency in such systems. A tidal flow operational strategy enhanced aeration and was coupled with a step-feeding approach to promote N removal. Over the 420-day running period N removal rates were between 70 and 77 gN/m3/d, for a step-feeding ratio range of 60:40 to 80:20. The system was able to remove 91–95% of chemical oxygen demand, 74–91% of ammonium and 66–81% of total-N. Tidal flow and step-feeding strategies significantly impacted nitrogen removal with the best performance at a step-feeding ratio of 80:20 providing a carbon to nitrogen (COD/N) ratio of 4–5. The bacterial diversity increased at each stage throughout the system with dominating phyla Proteobacteria, Firmicutes, Planctomycetes, Bacteroidetes, Chloroflexi, Verrucomicrobia and Acidobacteria. Dominant bacteria at the genus level were Thiothrix, Planctomyces, Azonexus, Pseudoxanthomonas, Hydrogenophaga, Gemmobacter and other genera suggesting that N removal was accomplished via diverse metabolic pathways, including autotrophic nitrification, heterotrophic denitrification, autotrophic denitrification, and possibly anammox. This study shows benefits of step-feeding strategies in tidal flow constructed wetlands as a cost-effective solution for minimizing external carbon input to achieve effective N removal.
The SunPy Project: An interoperable ecosystem for solar data analysis
Authors:   The SunPy Community, Barnes Will T. , Christe Steven , Freij Nabil , Hayes Laura A. , Stansby David , Ireland Jack , Mumford Stuart J. , Ryan Daniel F. , Shih Albert Y.
Published: February 2023

The SunPy Project is a community of scientists and software developers creating an ecosystem of Python packages for solar physics. The project includes the sunpy core package as well as a set of affiliated packages. The sunpy core package provides general purpose tools to access data from different providers, read image and time series data, and transform between commonly used coordinate systems. Affiliated packages perform more specialized tasks that do not fall within the more general scope of the sunpy core package. In this article, we give a high-level overview of the SunPy Project, how it is broader than the sunpy core package, and how the project curates and fosters the affiliated package system. We demonstrate how components of the SunPy ecosystem, including sunpy and several affiliated packages, work together to enable multi-instrument data analysis workflows. We also describe members of the SunPy Project and how the project interacts with the wider solar physics and scientific Python communities. Finally, we discuss the future direction and priorities of the SunPy Project.
Scalable coupled aquaponics design: Lettuce and tilapia production using a parallel unit process approach
Authors: Tetreault Joseph , Fogle Rachel L. , Guerdat Todd
Published: February 2023

Coupled aquaponics is the integration of recirculating aquaculture systems (RAS) with hydroponic cropping systems (HCS) into a single system with shared water treatment units. Potential benefits of integration include water conservation, reduced reliance on finite mineral fertilizers, and intensive year-round location-independent production of lean proteins and fresh vegetables. However, coupled aquaponic practitioners have found minimal commercial success to date. This has been mostly due to the use of system designs which are not based on contemporary water treatment principles, especially those for commercial aquaculture. Instead, conventional coupled aquaponic system design has been based on a linear framework assuming fish wastes are readily utilized as plant fertilizers, with minimal emphasis on waste treatment or individual component hydraulic retention times. The result has been economic failures due to misbalancing the cost of inputs, the value of the outputs, and the time required to reach a marketable harvest size for both crops: fish and plants. This manuscript provides theoretical calculations based on existing standards in commercial RAS and HCS for sizing plant, fish, and biofiltration units focused on nitrogenous waste production from fish. Successful integration of HCS and RAS is defined as achieving industry standard production timelines for lettuce (seed to harvest time of 35 days) and Nile tilapia (fry to a 624 g average harvest weight in 35 weeks). Equations and examples to calculate lettuce yield, daily lettuce nitrogen requirement, fish feed rates to achieve specific nitrogen production rates, and fish tank and biofilter volumes are provided.
Modeling the accuracy of a novel PCR and antibody ELISA for African swine fever virus detection using Bayesian latent class analysis
Authors: Schambow Rachel , Giménez-Lirola Luis G. , Hanh Vu Duc , Huong Lai Thi Lan , Lan Nguyen Thi , Trang Pham Hong , Luc Do Duc , Bo Ha Xuan , Chuong Vo Dinh , Rauh Rolf , Nelson William , Mora-Díaz Juan Carlos , Rovira Albert , Culhane Marie R. , Perez Andres M.
Published: February 2023

Introduction

Diagnostic test evaluation for African swine fever (ASF) in field settings like Vietnam is critical to understanding test application in intended populations for surveillance and control strategies. Bayesian latent class analysis (BLCA) uses the results of multiple imperfect tests applied to an individual of unknown disease status to estimate the diagnostic sensitivity and specificity of each test, forgoing the need for a reference test.

Methods

Here, we estimated and compared the diagnostic sensitivity and specificity of a novel indirect ELISA (iELISA) for ASF virus p30 antibody (Innoceleris LLC.) and the VetAlert™ ASF virus DNA Test Kit (qPCR, Tetracore Inc.) in field samples from Vietnam by assuming that disease status 1) is known and 2) is unknown using a BLCA model. In this cross-sectional study, 398 paired, individual swine serum/oral fluid (OF) samples were collected from 30 acutely ASF-affected farms, 37 chronically ASF-affected farms, and 20 ASF-unaffected farms in Vietnam. Samples were tested using both diagnostic assays. Diagnostic sensitivity was calculated assuming samples from ASF-affected farms were true positives and diagnostic sensitivity by assuming samples from unaffected farms were true negatives. ROC curves were plotted and AUC calculated for each test/sample combination. For comparison, a conditionally dependent, four test/sample combination, three population BLCA model was fit.

Results

When considering all assumed ASF-affected samples, qPCR sensitivity was higher for serum (65.2%, 95% Confidence Interval [CI] 58.1–71.8) and OF (52%, 95%CI 44.8–59.2) compared to the iELISA (serum: 42.9%, 95%CI 35.9–50.1; OF: 33.3%, 95%CI 26.8–40.4). qPCR-serum had the highest AUC (0.895, 95%CI 0.863–0.928). BLCA estimates were nearly identical to those obtained when assuming disease status and were robust to changes in priors. qPCR sensitivity was considerably higher than ELISA in the acutely-affected population, while ELISA sensitivity was higher in the chronically-affected population. Specificity was nearly perfect for all test/sample types.

Discussion

The effect of disease chronicity on sensitivity and specificity could not be well characterized here due to limited data, but future studies should aim to elucidate these trends to understand the best use of virus and antibody detection methods for ASF. Results presented here will help the design of surveillance and control strategies in Vietnam and other countries affected by ASF.
Cutaneous filarioid nematodes of dogs in the United States: Are they emerging, neglected, or underdiagnosed parasites?
Authors: Gruntmeir Jeff , Kelly Maureen , Ramos Rafael Antonio Nascimento , Verocai Guilherme Gomes
Published: February 2023

Filarioid nematodes, which are vector-borne parasites of cosmopolitan distribution, of dogs are medically important. They are represented by species in which microfilariae were found to be circulating in the bloodstream (e.g.,Dirofilariasp.,Acanthocheilonemasp., andBrugiasp.) or skin-dwelling (e.g.,Cercopithifilariasp. andOnchocercasp.). Those species whose microfilariae are detected in blood have been extensively studied, especiallyDirofilaria immitis, due to their clinical importance. In recent decades, there has been an increased interest by the scientific community in filarioid nematodes whose microfilariae are detected in the skin because of the zoonotic aspect ofOnchocerca lupi. In the United States (US), althoughD. immitishas been considered the main filarioid infecting dogs, the intense animal movement and global canine filarioid diversity may indicate that the likely presence of cutaneous filarioid nematodes is more common than previously expected. Hence, a question remains: Are these canine filarioid nematodes emerging, neglected, or simply underdiagnosed in the US? In this review, we provide an overview of pertinent information that briefly summarizes the biology of the different canine filarioid nematode species, clinical signs associated with infections, and currently available diagnostic tools using molecular and microscopy-based methods and highlight knowledge gaps where research and surveillance efforts remain necessary. The data herein presented serve as an alert to the scientific community about the importance of filarioid nematodes infecting dogs other thanD. immitis. Additionally, the zoonotic potential of several filarioid species reinforces the necessity of a proper diagnosis and the need for broader surveillance to understand their diversity and distribution, to highlight the potential introduction of certain species, and mitigate their establishment in the country and new animal and human cases.
Manufacturing Process for Multilayer Dielectric Elastomer Transducers Based on Sheet-to-Sheet Lamination and Contactless Electrode Application
Authors: Krüger Tim Simon , Çabuk Ozan , Maas Jürgen
Published: February 2023

Dielectric elastomer transducers (DETs) consist of thin elastomer films and compliant conductive electrodes on each side. Several DE-based systems, e.g., DE actuators, loudspeakers or sensors, have great potential in industrial applications. Different manufacturing processes for DE-based transducers have been realized so far. An alternative manufacturing process, based on sheet-to-sheet lamination of pre-fabricated elastomer films, appears promising for the manufacturing of large numbers of DETs, as, on the one hand, the commercially available elastomer films produced are characterized by homogeneous and reproducible properties and, on the other hand, the curing time during processing can be avoided. However, the handling of thin elastomer films and a controlled film transfer onto a surface is a challenge, as pre-stretch and wrinkles can easily occur. The presented sheet-to-sheet lamination mechanism facilitates controllable film handling well, whereby a high number of DE layers can be achieved. This paper focuses on the developed, automated film lamination of pre-cut film sheets and the electrode application via a jetting system, representing core components of the corresponding overall manufacturing process of DE laminates. The process realization on a laboratory scale is presented and manufactured DET specimens investigated for validation purposes.
Physiological Alterations and Nondestructive Test Methods of Crop Seed Vigor: A Comprehensive Review
Authors: Xing Muye , Long Yuan , Wang Qingyan , Tian Xi , Fan Shuxiang , Zhang Chi , Huang Wenqian
Published: February 2023

Seed vigor is one of the essential contents of agricultural research. The decline of seed vigor is described as an inevitable process. Recent studies have shown that the oxidative damage caused by reactive oxygen species (ROS) is the main reason for the destruction of various chemicals in seeds and eventually evolves into seed death. The traditional vigor tests, such as the seed germination test and TTC staining, are commonly used to assess seed vigor. However, these methods often need a large number of experimental samples, which will bring a waste of seed resources. At present, many new methods that are fast and nondestructive to seeds, such as vibrational spectroscopic techniques, have been used to test seed vigor and have achieved convincing results. This paper is aimed at analyzing the microchanges of seed-vigor decline, summarizing the performance of current seed-vigor test methods, and hoping to provide a new idea for the nondestructive testing of a single seed vigor by combining the physiological alterations of seeds with chemometrics algorithms.
Application of Traditional Cooking Methods in Chestnut Processing: Effects of Roasting and Boiling on Secondary Metabolites and Antioxidant Capacity in Castanea spp. Fruits
Authors: Donno Dario , Mellano Maria Gabriella , Carini Valeria , Bergamasco Elena , Gamba Giovanni , Beccaro Gabriele Loris
Published: February 2023

More information on the effects of traditional cooking methods (roasting or boiling) on the chestnut composition may be important if health-promoting aspects are considered. The main aims of this study were to investigate and describe the phenolic profile and antioxidant capacity of raw, boiled, and roasted chestnuts from several Castanea spp. genotypes, evaluating the influence of the application of different traditional cooking methods on the nut phytochemical composition by chromatographic and spectroscopic strategies. The amounts of phenolics were used as selected variables together with total polyphenol content and antioxidant capacity to perform a Principal Component Analysis (PCA). Catechins and tannins were the main molecules in the phenolic phytocomplex, reaching 30–40% of the total, followed by phenolic acids (5–20%) and flavonols (about 5%). Gallic and ellagic acids were the most important phenolic acids in raw and processed chestnuts (about 20–70 mg·100 g−1 dried weight-DW and 10–50 mg·100 g−1 DW, respectively). Both of the cooking processes significantly influenced the polyphenolic content and the relative antioxidant capacity. This research may support and confirm the potential use of chestnuts for human health, increasing the information on the phenolic pattern of differently processed Castanea spp. fruits from different genotypes to (i) assess the potential health-positive effects, (ii) help processing companies to select specific varieties to commercialise in the market, and (iii) increase the use of these fruits with the relative increase in income for the producers.
Role of Arbuscular Mycorrhizal Fungi and Phosphate Solubilizing Bacteria in Improving Yield, Yield Components, and Nutrients Uptake of Barley under Salinity Soil
Authors: Masrahi Abdurrahman S. , Alasmari Abdulrahman , Shahin Mostafa G. , Qumsani Alaa T. , Oraby Hesham F. , Awad-Allah Mamdouh M. A.
Published: February 2023

Barley (Hordeum vulgare, L.) is the fourth most important cereal crop in the world. Salinity decreases the productivity of plants grown under salinity conditions. It leads to deficiency and limited absorption of water and nutrients, ionic stress, oxidative stress, and osmotic imbalance. In saline soil, a field experiment was conducted to verify the effects of nine combinations among three levels of bio-fertilizers, i.e., control (without), arbuscular mycorrhizal fungi (AMF), and phosphate solubilizing bacteria (PSB), as well as three levels of phosphorus fertilizer recommended dose (RDP) on barley yield, its components and nutrients uptake, to evaluate the useful influences of these combinations to improve P management under salinity stress related to yield and its components as well as N, P, and K uptake in barley. Findings revealed that the combination AMF + 100% RDP improved plant height, length of spike, spikes weight, number of spikes plant−1, weight of 1000-grain, straw yield, grain yield, uptake of nitrogen (N), phosphorus (P), potassium (K) in grain and uptake of nitrogen (N), phosphorus (P), potassium (K) in straw by 19.76, 33.21, 40.08, 33.76, 14.82, 24.95, 47.52, 104.54, 213.47, 168.24, 124.30, 183.59, and 160.84% in the first season, respectively. Meanwhile, the increase was 19.86, 29.73, 40.47, 39.94, 14.92, 24.95, 47.94, 104.73, 213.33, 168.64, 124.47, 183.86, and 161.09% in the second season, respectively. AMF showed greater efficiency and effectiveness compared to PSB in improving yield and its components for all studied traits. The results of principle component analysis indicated that all combinations except AMF + zero% RDP, PSB + zero% RDP, control + zero% RDP, and control + 66% RDP showed high scores on positive PC1, where all studied traits were high. Therefore, it is recommended to inoculate the soil with AMF or PSB with the addition of phosphate fertilizer at the recommended dose under salinity conditions, i.e., AMF + 100% RDP (T1) or AMF + 66% RDP (T2) or PSB + 100% RDP (T4). The use of bio-fertilizers has increased plant tolerance to salt stress, and this was evident from the increase in different traits with the use of treatments that include bio-fertilizers.
Survival and Demography of the Tomato Borer (Tuta absoluta) Exposed to Citrus Essential Oils and Major Compounds †
Authors: de Paiva Silva Gabriel Tadeu , Figueiredo Karolina Gomes , Alves Dejane Santos , de Oliveira Denilson Ferreira , Silva Geraldo Humberto , de Souza e Silva Gabriela Trindade , de Oliveira Murilo Silva , Biondi Antonio , Carvalho Geraldo Andrade
Published: February 2023

Tuta absoluta is a pest of importance: quick to disperse and difficult to control due to the cases of resistance to insecticide active ingredients. Thus, studies using essential oils (EOs) to search for new molecules should be intensified. The objective of the present study was to evaluate the toxicity of EOs from Citrus aurantifolia (lime), Citrus aurantium (petitgrain) and Citrus aurantium bergamia (bergamot) and its major compounds against T. absoluta in a topical application test. Additionally, the demographic parameters of T. absoluta were studied after the topical application of EOs. The median lethal time (LT50) of the population was 12h for the three EOs tested. The median lethal concentration (LC50) was 33.75, 38.78 and 35.05 µg µL−1 for C. aurantifolia, C. aurantium and C. aurantium bergamia, respectively. As found using gas chromatography coupled to mass spectrometry (GC-MS) quantification, 44.74% of the EO of C. aurantifolia is α-terpineol, while 55.45% and 58.12% of the EO of C. aurantium and C. aurantium bergamia, respectively, is linalyl acetate. The toxicity of the major compounds was tested at concentrations equivalent to the LC50 of the EOs, that is, 16.2 µg µL−1 for α-terpineol, and 25.8 µg µL−1 for linalyl acetate, using topical application. Both of the major compounds showed less toxicity than the EOs. In the sublethal effects tests, all the EOs negatively affected the demographic parameters of T. absoluta, with a decrease in the duration of larval instars, duration of the pupal period, fecundity, oviposition and viability of the eggs, implying a reduction in the population growth parameters of this pest. The EOs of lime, petitgrain and bergamot are toxic to T. absoluta, and low concentrations cause deleterious effects on the reproductive and population parameters of T. absoluta.
Effects of Water Stress on Resveratrol Accumulation and Synthesis in ‘Cabernet Sauvignon’ Grape Berries
Authors: Sun Yanli , Xi Ben , Dai Hongjun
Published: February 2023

Resveratrol (3, 4′, 5 trihydroxy stilbene) is a natural phytoalexin produced by plants in response to biotic and abiotic stresses. It is well known for its cardio-protective, anticarcinogenic, and antioxidant properties. This study characterized physiological and molecular changes in resveratrol synthesis exposed to two levels of water stress at distinct grape berry developmental stages. Physiological data were measured to assess the berry quality. We used high-performance liquid chromatography to study enzyme activity and qRT-PCR to assess the expression levels of genes involved in resveratrol synthesis. The berry development was suppressed under water stress, while the content of total polyphenol, especially resveratrol was enhanced. Related enzymes and genes regulate the changes in resveratrol in plants. Water stress improved the enzyme activities of PAL (phenylalanine ammonia-lyase) and STS (stilbene synthase) after veraison. Moreover, the transcription factors VvMYB14, VvMYB15 and resveratrol synthesis-related genes are also changed. Expression of Vv4CL and VvMYB15 were upregulated during the pre-reversion stage, whereas VvPAL and VvSTS increased throughout development. As the co-substrate of VvSTS, VvCHS decreased during the berry development. Our study demonstrates that water stress regulates resveratrol synthesis through enzymatic activities and the gene expression of PAL and STS.
A Preliminary Study of the Influence of High Intensity Laser Therapy (HILT) on Skin Surface Temperature and Longissimus Dorsi Muscle Tone Changes in Thoroughbred Racehorses with Back Pain
Authors: Zielińska Paulina , Soroko-Dubrovina Maria , Dudek Krzysztof , Ruzhanova-Gospodinova Iliana Stefanova
Published: February 2023

The reason for undertaking this study was to investigate soft tissue response to high-intensity laser therapy (HILT) by measuring changes in skin surface temperature and longissimus dorsi muscle tone in the thoracolumbar back area in Thoroughbreds with back pain and diagnosed with and without Kissing Spines Syndrome (KSS). Thoroughbreds aged 3–4 years with clinically presented back pain underwent a radiological examination (to assess a lack or presence of KSS) and longissimus dorsi muscle palpation (to assess muscle tone and pain degree). The subjects were divided into two groups, those with KSS (n = 10) and those without KSS (n = 10). A single HILT treatment on the longissimus dorsi muscle, on the left side, was performed. Thermographic examination and palpation were repeated before and after HILT to assess changes in skin surface temperature and muscle pain response. In both groups, HILT caused a significant increase in skin surface temperature of 2.5 °C on average and a palpation score reduction of 1.5 degrees on average (p = 0.005 for both measurements), without differences in any outcome measures between the groups. Furthermore, the correlation between changes in the average skin surface temperature and the average palpation scores in horses with and without KSS were negative (rho = 0.071 and r = −0.180, respectively; p > 0.05). The results of the present study are encouraging, but further studies with larger samples, a longer follow-up period and comparisons with placebo control groups are needed to draw a more valid conclusion.
Locomotion as a Measure of Well-Being in Captive Chimpanzees (Pan troglodytes)
Authors: Neal Webb Sarah , Schapiro Steven
Published: February 2023

Locomotion in non-human primates, including walking, climbing, and brachiating among other types of movement (but not pacing), is a species-typical behavior that varies with age, social housing conditions, and environmental factors (e.g., season, food availability, physical housing conditions). Given that captive primates are typically observed to engage in lower levels of locomotor behaviors than their wild counterparts, increases in locomotion are generally considered to be indicative of improved welfare in captivity. However, increases in locomotion do not always occur with improvements in welfare, and sometimes occur under conditions of negative arousal. The use of time spent in locomotion as a welfare indicator in studies of well-being is relatively limited. We conducted focal animal observations on 120 captive chimpanzees across a series of studies and found higher percentages of time spent in locomotion (1) upon transfer to a new enclosure type, (2) in larger groups with wider within-group age ranges, and fewer males, and (3) with participation in an experimental medication choice paradigm. We also found that, among geriatric chimpanzees, those housed in nongeriatric groups exhibited more locomotion than those living in geriatric groups. Lastly, locomotion was significantly negatively correlated with several indicators of poor welfare and significantly positively correlated with behavioral diversity, one indicator of positive welfare. Overall, the increases in time spent in locomotion observed in these studies were part of an overall behavioral pattern indicative of enhanced welfare, suggesting that an increase in time spent in locomotion itself may be an indicator of enhanced welfare. As such, we suggest that levels of locomotion, which are typically assessed in most behavioral experiments, may be used more explicitly as indicators of welfare in chimpanzees.
The Development of Smart Dairy Farm System and Its Application in Nutritional Grouping and Mastitis Prediction
Authors: Hu Tingting , Zhang Jinmen , Zhang Xinrui , Chen Yidan , Zhang Renlong , Guo Kaijun
Published: February 2023

In order to study the smart management of dairy farms, this study combined Internet of Things (IoT) technology and dairy farm daily management to form an intelligent dairy farm sensor network and set up a smart dairy farm system (SDFS), which could provide timely guidance for dairy production. To illustrate the concept and benefits of the SDFS, two application scenarios were sampled: (1) Nutritional grouping (NG): grouping cows according to the nutritional requirements by considering parities, days in lactation, dry matter intake (DMI), metabolic protein (MP), net energy of lactation (NEL), etc. By supplying feed corresponding to nutritional needs, milk production, methane and carbon dioxide emissions were compared with those of the original farm grouping (OG), which was grouped according to lactation stage. (2) Mastitis risk prediction: using the dairy herd improvement (DHI) data of the previous 4 lactation months of the dairy cows, logistic regression analysis was applied to predict dairy cows at risk of mastitis in successive months in order to make suitable measurements in advance. The results showed that compared with OG, NG significantly increased milk production and reduced methane and carbon dioxide emissions of dairy cows (p < 0.05). The predictive value of the mastitis risk assessment model was 0.773, with an accuracy of 89.91%, a specificity of 70.2%, and a sensitivity of 76.3%. By applying the intelligent dairy farm sensor network and establishing an SDFS, through intelligent analysis, full use of dairy farm data would be made to achieve higher milk production of dairy cows, lower greenhouse gas emissions, and predict in advance the occurrence of mastitis of dairy cows.
Editorial: Role of Non-Coding RNAs in Animals
Authors: Do Duy Ngoc , Suravajhala Prashanth
Published: February 2023

The importance of non-coding RNAs (ncRNAs), such as microRNAs (miRNA), long non-coding RNAs (lncRNA), and circular RNAs (circRNA), in gene regulation is increasingly being appreciated in many species [...]
Effect of Quorum Sensing Molecule Farnesol on Mixed Biofilms of Candida albicans and Staphylococcus aureus
Authors: Gaálová-Radochová Barbora , Kendra Samuel , Jordao Luisa , Kursawe Laura , Kikhney Judith , Moter Annette , Bujdáková Helena
Published: February 2023

The natural bioactive molecule farnesol (FAR) is widely studied mainly for its antibiofilm and antimicrobial properties. In addition, it increases the effectiveness of some antimicrobial substances, which makes it interesting for the development of combined therapy. In the present work, the effect of FAR either alone or in combination with oxacillin (OXA) on mixed biofilms formed by clinically relevant pathogens, Candida albicans and Staphylococcus aureus, was studied. S. aureus isolates used for biofilm formation originated from blood cultures and central venous catheters (CVC) were characterized in terms of antimicrobial resistance. The minimal biofilm inhibitory concentration (MBIC50) for FAR of 48 h mixed biofilms formed by the C. albicans and methicillin-sensitive S. aureus (MSSA) was determined to be 125 μM, and for the mixed biofilms with methicillin-resistant S. aureus (MRSA) was determined to be 250 μM. Treatment of mixed biofilms with OXA (2 mg/mL) showed ≤4% inhibition; however, the combination of OXA (2 mg/mL) and FAR (300 μM) resulted in 80% inhibition of biofilms. In addition, planktonic cells of S. aureus exhibited an increased susceptibility to OXA, cefoxitin and kanamycin in the presence of FAR (150 and 300 μM). Scanning electron microscopy (SEM) micrographs confirmed patchy biofilm and lack of candidal hyphae in the samples treated with FAR and FAR/OXA in comparison to control and mixed biofilms treated only with OXA. Intriguingly, in a pilot experiment using fluorescence in situ hybridization (FISH), considerable differences in activity (as indicated by ribosome content) of staphylococcal cells were detected. While the activity rate of the staphylococci in mixed biofilms treated with FAR was high, no FISH-positive signal for staphylococcal cells was found in the biofilm treated with FAR/OXA.
Antibiotic Resistance of Streptococcus pneumoniae in the Nasopharynx of Healthy Children Less than Five Years Old after the Generalization of Pneumococcal Vaccination in Marrakesh, Morocco
Authors: Amari Sara , Warda Karima , Bouraddane Majda , Katfy Mostafa , Elkamouni Youssef , Arsalane Lamiae , Zerouali Khalid , Zouhair Said , Bouskraoui Mohamed
Published: February 2023

Streptococcus pneumoniae (S. pneumoniae) remains one of the most important pathogens causing childhood infections. The spread of antibiotic-resistant bacteria is a leading cause of treatment failure in children. The purpose of this investigation is to report the antibiotic and multidrug resistance (MDR) of S. pneumoniae strains isolated from healthy children throughout the years 2020–2022. Antimicrobial susceptibility testing of S. pneumoniae strains in selected antimicrobials was performed using disk diffusion and E-test methods on bloodMueller–Hinton agar. The antimicrobials tested included oxacillin, amoxicillin, ceftriaxone, norfloxacin, gentamicin, vancomycin, erythromycin, clindamycin, pristinamycin, tetracycline, chloramphenicol, and trimethoprim-sulfamethoxazole. A total of 201 S. pneumoniae strains were isolated from the nasopharynx of healthy children in Marrakesh, Morocco. The highest rate of resistance of S. pneumoniae was found in penicillin (57.2%), followed by tetracycline (20.9%), and erythromycin (17.9%). The rates of resistance to clindamycin, trimethoprim-sulfamethoxazole, and chloramphenicol were 14.9%, 4%, and 1.5%, respectively. All isolates were susceptible to norfloxacin, gentamicin, vancomycin, and pristinamycin. Approximately 17% of all S. pneumoniae strains were resistant to at least three different antibiotic families. This study showed a low rate of antibiotics resistance among nasopharyngeal S. pneumoniae strains, and it is thus essential to monitor S. pneumoniae susceptibility in healthy children.